LOCUS       DASTKU010000001         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_223101, whole genome shotgun sequence.
ACCESSION   DASTKU010000001 DASTKU010000000
VERSION     DASTKU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223101"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VFQ61_00005"
     CDS             <1..714
                     /locus_tag="VFQ61_00005"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9952847.1"
                     /translation="VVSWNIHKGIGGVDRRYDIERTVGVLSEQEPDVALLQEVAEGMP
                     RAGFHDQAEFLSEALEMRHIAYGPQHRFRVGGYGNAILSRFPLTDIHHVDLTIGWRKK
                     RGVTAARAHVRSEGHTRSVVIFNVHLGLAGSERARQLQRFLECHPFAGLHARTPVVLG
                     GDLNDLWGSLGPRFLVPAGFSRAGALHNTFPAMLPLRPLDGIFIRGDVRSTACAPRVT
                     AVSRAASDHLPLFAELFLH"
     gene            821..>2207
                     /gene="pgi"
                     /locus_tag="VFQ61_00010"
     CDS             821..>2207
                     /gene="pgi"
                     /locus_tag="VFQ61_00010"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET9952848.1"
                     /translation="MTARTDFVQLPAFRALEAHYETLRQLELRNLFESDPTRGQRLSV
                     EACGIYLDYSKNRLTDETLGLLVRLAEESGVPAHRDAMFRGERINNTENRSVLHVALR
                     AARGAEILVDGKNVVPDVHAVLDRMGAFSDRVRSGAHLGHTGKRIRNIVNIGIGGSDL
                     GPVMAYEALRHYSDRNLNLRFVSNIDGTDLSEALIGLDAAETLFIICSKTFTTLETLV
                     NAQSARSWLLKALGDEQAVGKHFVAVSTNAEEVKKFGIELENMFGFWDWVGGRYSYAS
                     AVGLALMLGIGKQHFADMLSGFREMDEHFRSAPLERNLPVLLGLIGILYNNFFGAESC
                     AVLPYDQYLGRFPAYLQQLDMESNGKRVDREGHAVSYQTGPIVWGQPGTNGQHAFYQL
                     IHQGTKLVPCDFIGFCRSLNPMGSHHDHLMSNLIAQTEALAFGKTESEVAAEGVRADL
                     LTHRTFPGNRPT"
BASE COUNT          389 a          687 c          700 g          431 t
ORIGIN      
        1 gtcgtgagct ggaacattca caaaggcatc ggtggcgtcg atcggcgcta cgatatcgag
       61 cgaaccgtcg gggttttgtc cgaacaggaa ccggacgtcg cgctgcttca ggaggtggcg
      121 gaaggcatgc cgagagccgg ctttcatgat caagccgagt tcctgagcga agcgttggag
      181 atgcgtcaca tcgcctacgg cccacagcac cgcttcagag tgggtggcta cggtaacgcc
      241 atcttgagcc ggttcccgtt gacggacatt caccacgtcg atctcacgat tggctggcgc
      301 aagaagcgcg gggtgaccgc ggcacgcgcc cacgtgagga gcgagggaca cacccgcagc
      361 gtggtgattt tcaatgtgca cctggggctc gcgggctcag agcgggctcg gcagctccag
      421 cgcttcttgg agtgccaccc gttcgcaggc ctgcacgctc gtacgccggt ggttttgggc
      481 ggagacctga atgatctttg gggctcgttg ggaccccgct ttttggtgcc cgctggcttc
      541 tctcgagccg gcgcgctcca caacaccttt cccgccatgc tcccgctcag gccactggac
      601 gggatcttca ttcggggtga cgtgcgttcg acagcatgcg ccccgcgcgt gaccgcggtc
      661 agccgcgctg catctgacca tttgccgttg tttgcagagt tgtttctgca ctgacccgca
      721 ttggcgcgct gcaccgcgcc gctcccatgt cccgggcggg tttgctccgc ggcgtcgcgg
      781 ctggccgtgg agcgcgtgtt gctctaggct gcgcgccgcc atgactgcac gtaccgattt
      841 cgtccaactc cccgcgttcc gagccctcga agcccactat gaaacgctgc gccagctcga
      901 gctgcggaat ctattcgaga gcgacccaac gcgtggacag cgcctcagcg tcgaagcttg
      961 cggcatctac ctggattact cgaagaaccg gcttaccgac gaaaccctcg gtctactcgt
     1021 gcggctggcc gaggagtctg gcgtcccggc ccaccgcgat gcgatgtttc gcggtgagcg
     1081 catcaacaac accgagaatc ggtccgtgtt gcacgttgcg ctgcgcgccg cgcgcggcgc
     1141 cgagatcctg gtcgatggca agaacgtggt cccggacgtg cacgcggtgc tcgatcgcat
     1201 gggcgcattc agcgatcggg tgcgctccgg ggcccacttg ggccacaccg gcaagcgcat
     1261 tcgcaacatc gtgaacatcg ggattggcgg ctccgatttg gggccggtga tggcctacga
     1321 agccttgcgt cactacagcg atcgcaatct caatctacgc ttcgtctcca acatcgatgg
     1381 caccgatctg agcgaggcct tgattggcct ggacgcagcg gagacgctgt tcatcatctg
     1441 ctccaagacc ttcaccaccc tcgagacgct ggtcaacgct cagagcgcgc gaagttggtt
     1501 gctgaaagcg ctcggggacg agcaggccgt cggcaagcac ttcgtggccg tgagtaccaa
     1561 cgccgaggaa gtgaagaaat tcggcatcga gctagagaac atgttcgggt tttgggactg
     1621 ggtcggcggg cgctactcgt acgcttcggc ggtcgggctc gcgctcatgc tcggcatcgg
     1681 caaacaacat ttcgcggaca tgctcagcgg cttccgcgaa atggacgagc acttccgaag
     1741 cgcgccgctg gagcgcaact tgccggtgct gctggggctg attgggattc tctacaacaa
     1801 cttcttcggc gcggagtcct gcgcggtgct gccgtacgat cagtacctgg ggcgttttcc
     1861 cgcatatctg cagcagctcg acatggagag caatggcaag cgtgtggacc gcgaggggca
     1921 cgccgtcagt tatcaaacgg gacccatcgt ttggggccag cccggcacca atgggcaaca
     1981 cgccttttat cagctgattc accaaggcac caagctcgtg ccttgtgact tcatcgggtt
     2041 ctgccgcagc ctgaacccga tgggttcaca ccacgatcat ttgatgtcga acctgattgc
     2101 gcagaccgaa gcgctggcgt tcgggaaaac ggagtccgag gtcgccgcgg aaggcgtgcg
     2161 cgccgatctg ctcacgcatc gtactttccc gggcaatcgt ccgacga
//
LOCUS       DASTKU010000002        13040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1115232, whole genome shotgun sequence.
ACCESSION   DASTKU010000002 DASTKU010000000
VERSION     DASTKU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13040
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115232"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFQ61_00015"
     CDS             <1..377
                     /locus_tag="VFQ61_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952849.1"
                     /translation="TQTALSVRKRFRQLFVQGEYSPLETFGHALAFARTFGSESIVVC
                     VPRLPYRLTQGKPSWPMGKVWRAESVVLPVGRYRDAFTGRTLVSNGSVKLAVCFAAFP
                     VALLISEPAAINEPADALEKSG"
     gene            777..1889
                     /locus_tag="VFQ61_00020"
     CDS             777..1889
                     /locus_tag="VFQ61_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET9952850.1"
                     /translation="MTRSPLLHGADDRRDVASVGDRILRGLALESTLALVAKNDLCKS
                     ASSDDIDIQSSDLRSAEHLCDGEAFGAQPTLARCTGVLVDDDLVLTVAHCATNIEHCS
                     EQTWAFGYAITNPEQWPRLAREDLYRCRSIPVLRYETLPGGERFDYALVQLDRPVSAQ
                     RKPAVLASTPVISGEPLCVIGFPSGLPVKIDCGALAIDVRDAQQDFLQLTSDTFRGSS
                     GSGVFRAGGELVAVLARGGPDYDEVPGEGCRVGHRISDVSDLTGAEQAGYAAPAIREL
                     CDRGWASSRLCGTSPRCGDNQCSSPEHEAESAQACLQDCPVLPLTISEPVAVQGCQVT
                     ANTHRRWPTEPLVGLTLVSMLMIRHRVFRRADSPVA"
     gene            2017..2388
                     /locus_tag="VFQ61_00025"
     CDS             2017..2388
                     /locus_tag="VFQ61_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952851.1"
                     /translation="MKNSAKILFVLVGLMATITACSAESARTSEASELTPVDPTQLAL
                     SDDPGAPGEDPNLQDKGNGRVYIQYCDRPNNSAGTICRTYDGTINWSTDPDECRRDTQ
                     NVCGGPTATWILCAGPNCWYM"
     gene            complement(3068..4417)
                     /locus_tag="VFQ61_00030"
     CDS             complement(3068..4417)
                     /locus_tag="VFQ61_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952852.1"
                     /translation="MVDFSKAERVSVATLAAGFLGLTSCGNSVPDPGASGGMSTAAGG
                     RTAQATGGTMSSAGQSQVASGGTIGQGGSAGLGGGSTGGASGASTGGSSASGGSPATG
                     GNAFGGSTSGGGASGGATSGGVASGGAASGGAASGGAGAGGTASGGAGGVDTGGRNSG
                     GSASGGAAGGSTGGTFSGTCTASKPVNANVSGSGPHKVTVETNSESGIREGTIYRPTD
                     LGGTEKYPIFVWGNGACSQNGLSNTAAMGEIASHGYFVISDGTPNGGGSRSQTSDWVN
                     MGKPLVAYINWAIAENEKPCSAYYHALDTTKVASNGFSCGGLMSEGAAIDPRITAWGF
                     TSSGLTSIDQNHYKKIHAPTLIILGGTSDIAYKNGQNDFTGITALGIPTLLFSKNIGH
                     GGDLGNARGGDFTKINLAWLNWQLKGDEGATGKGVLVGSTCSYCKDSAWEVKSKNIE"
     gene            5265..6710
                     /locus_tag="VFQ61_00035"
     CDS             5265..6710
                     /locus_tag="VFQ61_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952853.1"
                     /translation="MWKSSMNELRKCVVRLETAGSPPIIAVRRIAGAGLLCLTGACSS
                     DAAPSSVSPPHAGATQSAEAHGGLGGASGVVGSTQASENGGAGGLGGANALGGAPALG
                     GTGTTTATPRGGTGGGFGGTSADPARGGSSGTGGLGGTTTAGAASGGITSSAGGATSG
                     QSYSVPRGASKGCGIRSFSDKPGEYTSHDIDVMGVDSYWLTMRVPYAGQAPYTFTHRS
                     FSVRLPRGYDPQKAYPLVFQGGGCGNTDGTSGKNGADKLVPDDASSEAIAVGLSYVYP
                     DGAGACFADDGENTYELPYWDAVYAAVTASYCVDLEKVFIGGFSSGAWMAYTVAFARA
                     GLVRGIGTGAGGMREKHPARSIIPFAAFMVTGLDDDANPIHRTKDGTSCTGSEADGCW
                     KGKLICGTPGAESCFDTGSAHARDEILKRNGCVGTDTVQHGEWSDCRRYTGCPAAFPV
                     VYCTPPGGHTSGDDRHNPGTWNLWKALPAVP"
     gene            7015..8175
                     /locus_tag="VFQ61_00040"
     CDS             7015..8175
                     /locus_tag="VFQ61_00040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9952854.1"
                     /translation="MALSAIACSSSTTPDTGAGGQVPGTSGGTSGTPASGGAGATSAG
                     GRGGATSSQTGGSTATGGSVSTGGANASGGSSSPSGGATTGMGGGAGLSGGGAGGTSG
                     AGGSSGGTDSKGGTSGSGAGGGNAGAGAGGKTTGESGGAGGRGGASGDGGTGGSNGGA
                     SGGSTSFRPCPANDPCKILPLGDSITHGLQSSDNAGYRSQLFKLAVADNKKLTFTGSQ
                     SSGPNQVSGATFPKNHEGHDGWTVDPGFSKYGSGGISSLIPSPALSTMPNIVLLMIGT
                     NDITSTDTPGATADRLDGFLGKLVQAAPDALIVVAQITPVAYDSADLKNYNSKIPGLV
                     QARVAKGQHLIGVDMSKMPTSGLASDKVHPNDQGYAYMANVWYEAIKGYLPN"
     gene            complement(8219..10000)
                     /locus_tag="VFQ61_00045"
     CDS             complement(8219..10000)
                     /locus_tag="VFQ61_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952855.1"
                     /translation="MSSPRALAALHRITWALVLLAHLVLGGLAWWLLPHGFPLLHWRT
                     GLGIVVPFAMVATSLGIGSLLLFEPRTAARWLPAIPAFWCSLALGLILIFPETGWTAA
                     RTLVLSGLFFAILAGFACRGERALALLPALLAGAAFGLLTALCEKPAPASTRAGPGRQ
                     PRLATEPREPILADWELAPDLVVEGPAARVTWRGRRTAVSISPVLTFDRISPDGFWSI
                     FAPFTGPASPAAATVAKDELRLWTADGVPMLAVRRDFTLPNAPTQVVQLESSTWLDRA
                     VYSHLNSFTTVMVSGHRRLGLRFSPCANSLIAVTHADYPSGAPARFAYADAGHRFHIA
                     QATDAEKGPFTTLAEGSLPRDATLAVDLVDMEEPEGAFARLEFLDFESQLSTALSPTA
                     GYGVPENAIQFGLESPNLESPAHLIFTLAASGIGRGWDSVAHAGGTYRNRIILRSLER
                     PPALTGSEPLHDLTASCTAIARDIDALKPNYPQLAAFDPGNVIAGNGECTISYAYRTH
                     RSTRRGGWVAQVPNPDPDGVWFHIGIWDPQGPGRNSQINTQPGFSVYRLAEDNVTFLA
                     LEGAKVKPLRPELRRVLERRGMGEMVR"
     gene            10111..10740
                     /locus_tag="VFQ61_00050"
     CDS             10111..10740
                     /locus_tag="VFQ61_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952856.1"
                     /translation="MALVKRTAHVLTLGFTAFWLVATSRAPGPVHPCFVPLEAKKVRI
                     ALGAAMPASPGAAPSCQGLDDLREGAVIGVGLIPSSDRTARVRVDQDDACLGYDVTEL
                     TGPVGLSQPHSGDPRYPVPRALFESRAHFSSASSAPRCAGEYHISVAPVNPLPRNALV
                     DPLDAGHREPWQLRRVIRVDTTRVCALSPALPPGDCQDIFEVRSLELGP"
     gene            10724..11185
                     /locus_tag="VFQ61_00055"
     CDS             10724..11185
                     /locus_tag="VFQ61_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952857.1"
                     /translation="MSSGPEASAPRSGEFFAPLPLAALALLVVNDVYLKAKFHNFVTG
                     KLSDLAVCFLMPLFLSELLGLTLGIAPRLRLTMGAATTAVLFSVLEVVPSAAAWSVNL
                     LSRIGPHLGLGCHYAMTADITDLACVPVVLLAYGYGWRRVLGVVRAPELSA"
     gene            complement(11175..11906)
                     /locus_tag="VFQ61_00060"
     CDS             complement(11175..11906)
                     /locus_tag="VFQ61_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952858.1"
                     /translation="MAPVLWLWLAIGCGESDPGNGGTGGATTKGGAGGSGNKGGAGGS
                     GGSTAGTAGTTGHRVGDSCRSGSGVVVGSRNDCFRDDAFCEDLGDGTFCTGGLIPQPC
                     PPGTHRSLPGAACIPDMVNGGAGGVDGAGGDTSNTAGLSGGGTHGGTDSDCVGPSGLP
                     GKIVEDQTDCYLDDAFCVARSDGTWCTGGQPFICPPGQIRIDWGTCGSAGGAGAGGDG
                     GSENGGASFAGAGGEEAGGTAGTTH"
     gene            complement(12050..12370)
                     /locus_tag="VFQ61_00065"
     CDS             complement(12050..12370)
                     /locus_tag="VFQ61_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952859.1"
                     /translation="MLRDLTPPQRALAEYMSELSEEAYCAGWMDNLEYTLWQVVLGER
                     RAYGRSSFRAEHIDRLKQLSHECAGWIVFDEKRDQEWIALQDWKLRFAAWLRTPAAKK
                     TATW"
     gene            12546..>13040
                     /locus_tag="VFQ61_00070"
     CDS             12546..>13040
                     /locus_tag="VFQ61_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952860.1"
                     /translation="MGTMISTPDKATARDQLLTQLGEEASTKLRLATVRLTAGLFTGS
                     GVILTTVIKGDRGVFVVATAAHNLWFYVDSLTGGTSRDFWGGDKGKWSAEVLSSKKQA
                     IIDLCLARLNVEYASADMTQPNPTAAVTTATIGRVILAEFQYDLCLLLIPFPLRARPR
                     CEIVA"
BASE COUNT         2423 a         4187 c         4061 g         2369 t
ORIGIN      
        1 tgacccagac ggcgctttca gtccgcaagc gcttccgaca gctattcgtt caaggggagt
       61 actccccact cgagacattc ggtcacgcat tggcctttgc ccgaaccttc ggctccgaat
      121 ccatcgtcgt gtgtgtgccg cggctgccgt accggctcac tcagggcaag ccctcctggc
      181 cgatgggcaa ggtgtggcgc gccgaatcgg tggtacttcc ggtgggccgt tatcgcgacg
      241 cattcaccgg tcgcaccctc gtgtcgaacg gttcggtaaa actggccgtg tgcttcgccg
      301 cctttccggt ggccctgttg atcagcgaac cggcagcgat taacgaaccc gctgacgcgc
      361 tggagaaatc gggctgagta gcgcgtagac gaagatctga acccagcggt gtcctctcgg
      421 ttgggttcag atgcttcgcc aactggctgc ctcgccaact ggatagtggt gcgctcaacc
      481 gcactgcacg cgtgaactgc ctcggcctgc ccgcagcgct caaaggggcc agcaacgtcc
      541 ggcgaaagaa aagtggaagc gatgcgtggg gccggacatt agcaccgtat ccgctaggcc
      601 acgccacgca gccgatgctt gccgtggact ggctcgcaaa ctgaagcgcc taaccgccgc
      661 gaacgccagt gaagcaacga gtgcaaaaag accagatacc gtgggcagcg cggcttttcg
      721 cgctcttctc cacgctgctc tccgcggtgg cttgtcagct gcagggggaa acagccgtga
      781 cgcgctcacc cctgctccac ggcgctgatg atcgtcgaga tgtggctagc gtgggagatc
      841 gaatcctacg agggcttgcg ctcgaaagca ctctagcact cgtcgcgaag aacgacttgt
      901 gcaagtcggc ctcttcggac gatatagaca tccaatcctc ggatctccgc tccgccgagc
      961 atttgtgtga cggtgaggca ttcggtgccc agcccacttt ggcacgctgt accggcgtgc
     1021 tggtcgacga cgacttggtg ctgacggtcg cgcattgcgc gacgaacatc gagcattgtt
     1081 cggagcagac ttgggccttc ggctatgcaa tcaccaatcc tgagcagtgg cctcgactag
     1141 cgcgggaaga cctgtatcgc tgtcgtagca tcccggtgct ccgctatgaa accctgccgg
     1201 gtggtgaacg cttcgactat gccttggtgc agctggatcg accggtgtcg gctcagcgca
     1261 aaccagcggt gctcgcgagc actccggtga tttctggcga gcccctgtgc gtgatcggat
     1321 ttccaagcgg cttacccgtc aaaatcgatt gcggcgcact cgcgatcgac gtccgtgatg
     1381 ctcaacagga ctttttgcaa ctcacgagcg ataccttccg cggtagcagc ggctcgggcg
     1441 tatttcgcgc cggcggcgaa ttggttgcgg tgctcgctcg gggcggacct gactacgatg
     1501 aggtgcccgg ggaagggtgc cgagtcggac ataggatttc agacgtctcg gatctgactg
     1561 gtgccgaaca ggcaggttac gccgcgcccg ccattcgaga gctctgcgac cgcggctggg
     1621 ccagctctag gctgtgcggc acgtcaccac gctgtgggga taaccagtgc tcgagccccg
     1681 agcacgaggc agagagcgca caggcgtgcc tgcaggactg cccggtattg ccactgacga
     1741 tctcagaacc cgtagccgtt cagggctgcc aagtcacggc caacactcac cgccgttggc
     1801 caacggagcc actcgtcggt ttaaccctgg tttctatgct catgatcaga caccgcgtct
     1861 ttcgacgagc agactcgccg gttgcctgat ttcgaattgc ggtctgacga ttttgtcgga
     1921 cctctgtaga caagccattg ccccatgcac atcgagccaa gacggtcgca agcacgcctg
     1981 tgctgcgctc gcccgctcga ggaaagaatc gtaatcatga agaacagcgc aaaaatcctg
     2041 tttgtcctcg ttggattgat ggccaccatc accgcctgca gtgccgagag cgcgcgaacg
     2101 agcgaagcct ccgagctcac ccccgtagac cccacacaat tggcgctcag cgacgatcca
     2161 ggcgcccccg gcgaagatcc aaacctccag gacaagggca acggacgagt ttacatccaa
     2221 tactgcgacc gacccaacaa cagtgcagga acgatctgca gaacttacga cggtacgata
     2281 aactggtcca ccgatccaga cgagtgccga agagacaccc agaatgtttg cggtggtcca
     2341 actgccactt ggatcctctg cgccggcccg aactgttggt atatgtagcg aaccgcagtc
     2401 gtcgcatgcg ccgccccgga actccggact tccggggcgg tgtcgtatat tctttgcgac
     2461 ggggaggtag cgttcgggcc gcttagcgcg cgagctgccc gcgcagcggc ccagcttctc
     2521 accactctct ggacggagat tcacaagcgc ggttgcgaaa gaagtctgcg cgtcgaatga
     2581 aggagctgaa tccgtcgcag gcgcccgcgc aaaactcggc ggcatcggca atgccgatcg
     2641 gaatgcaggt gtccagtgcc ccgtacggtc cgggacacct gctcgtgtaa ctccgacacg
     2701 cgcgcaagca gaggcgcagc gctgggagac agcgctgacg ttctctagcg cgttcgcgat
     2761 cctgttcgag caggaaccgc tcggggtgtt cgatgtcttc cgcagatgac attgggcgtg
     2821 gaaacctcgg acaatcttcc aatccctcga gccgctctcg ggtttgcgga ttcgtgcgcg
     2881 tccgcgatgc acaagaagag ccgagaacga gcatgacaat tagcagcaac gagagatttg
     2941 cgaattgcgc cacggcgggc ttcagaccat tggcgctacc cattcattgg gtagtgcccc
     3001 aactttctgc ggggccgccg cttccgaggt gcgtggggtt gccctgggga cgtcgagcgc
     3061 cgatccgcta ctcgatgttc ttcgacttta cttcccaggc gctgtccttg cagtaggagc
     3121 aggtcgagcc aacgagcacg cccttgccgg tcgcgccttc atcgcctttg agttgccaat
     3181 tgagccaggc gagattgatc ttggtgaagt cgccgccacg ggcattgccc aaatcgccgc
     3241 cgtggccgat gttcttgcta aatagcaaag tgggaatgcc gagtgcggtg atcccggtaa
     3301 agtcgttctg gccgtttttg taggcaatgt cgctcgtgcc acccagaatg atcagggtcg
     3361 gcgcgtggat cttcttgtag tggttctgat cgatgctggt gagtccgctg ctcgtgaatc
     3421 cccatgccgt aatgcgcgga tcgatcgcgg ccccttcgga catgaggcca ccacaagaga
     3481 acccattcga cgcgaccttg gtcgtgtcga gcgcatgata gtacgcgctg cacggcttct
     3541 cattttccgc aatggcccag ttgatgtacg caaccagcgg cttgcccatg ttgacccagt
     3601 cgctggtttg ggaacggctg ccaccgccgt tcggcgtacc gtccgagatc acgaagtagc
     3661 cgtgagaagc gatctcgccc atagcggctg tattcgacag gccgttctga gagcacgcgc
     3721 cgttgcccca gacgaatatt ggatattttt cggtgccccc caagtccgtg ggtcgataaa
     3781 tcgtgccctc gcgaatgcct gattcggagt tcgtctcgac ggttaccttg tgcggaccgc
     3841 tgccggatac gttcgcattc acgggtttcg acgcggtgca cgttcccgag aacgttccgc
     3901 cggtgctccc gcccgcggct ccaccactcg cgctgccgcc gctgttacgt ccgcccgtgt
     3961 ccacgccgcc ggctccgccg gaggctgttc cacccgcacc ggcgccgcca gaagctgcac
     4021 cgccagaggc cgcaccgccg gacgccaccc cgccggatgt tgctccgccg gaagctcctc
     4081 caccggacgt tgacccgccg aacgcgtttc cacccgtcgc cggagagccc ccggacgccg
     4141 aggatccacc cgtggatgct ccgctagcgc cgcccgtaga tccaccgcct agccccgccg
     4201 agccgccctg gccgatggtg cccccagatg caacctgcga ttgtccagcg ctgctcatgg
     4261 tgccacccgt cgcctgcgcc gttcgacccc cggcagcggt actcattccg ccactcgcgc
     4321 ccggatccgg aaccgagttc ccgcagcttg tcaggcccaa gaaacccgca gccaacgtcg
     4381 ctaccgaaac ccgctccgcc tttgaaaagt cgaccattct catcaaccag ctcctaactt
     4441 cttctgaagt ggcgttttct ctgcagccgc ggccaactcg gaaatggggt caaaaaactt
     4501 cgctcgagcc gggctcctgg gcagtgacgc gcgcggagcg gttccagcat cccgatcgta
     4561 aaatcgacga tctttactct gttattgccg tgattcagga agaatacgat ggcggcgaat
     4621 cggtgcgatt cgcaattcaa caccggttga gacagcgcct cagatcggtt cgctcatcaa
     4681 ccccgaacca atagcgtcgg cggctgctcc acgaaacgcc gccctgtcca cgggctgccc
     4741 gcgaacaccg aagctgctcc cccgcgaggg taacccaata gctcgccggt ccgtgctcgc
     4801 tagccgctgc gcgcgccccg aggccctcaa ggccatgctc gcttcgccga gcctccgctg
     4861 cgcgtggccg agggtcctgc cgccttagtg agtgtgaatc gagcggcgag gcgatccgag
     4921 ttcgcgctga cacgcgcgcc gcaagcagta ttcaatgccg caccatcttc gaagagcccg
     4981 ataaacacta ggtatgttgg gcggctagat agttggagtt gcagtcattc ccaatctgtc
     5041 gtcgaagcct gcttccgatc gagaccactg acctttaggc agcggacccc ggaattcgcg
     5101 cttatcagcg agtcgccaaa accacactcg accaccaaac ttcagggcgc gatcacttaa
     5161 tccaacgcgg gccgcgatgc gtccccacca cacggaacga gacggcccgt cgcgcgcacc
     5221 accttccagg cgcgctcaga atccgggccg tcgaagcatc ccgcatgtgg aaatcgagta
     5281 tgaacgagct gcgcaagtgt gtggtccggc tcgagacagc agggagtccg cccatcatcg
     5341 cggttcgcag gatcgcgggc gcgggcttat tgtgcctgac cggggcgtgc agtagcgacg
     5401 ctgcgccgag cagcgtttca cccccgcatg ctggcgctac tcaatccgcc gaagcacacg
     5461 gcgggctcgg tggggcgagc ggcgttgttg gctcgactca agcttccgag aacggcgggg
     5521 cgggcggtct cgggggcgcg aacgctctcg gcggcgcccc agcgctcgga ggcactggca
     5581 ccacgacagc aactcctcgg ggtggtactg ggggcggctt cggcggtacg agcgccgatc
     5641 ctgcaagggg cggcagcagc ggcactgggg gattgggcgg aaccacgacg gcaggtgccg
     5701 cgtccggagg catcacctcg agcgcaggtg gcgcgacgag cgggcagtca tattcagttc
     5761 cccgcggcgc gagcaagggc tgcggtattc ggagtttctc cgataagcca ggtgagtata
     5821 cgagtcatga catcgacgtg atgggcgtgg actcttactg gctcacgatg cgcgtcccgt
     5881 atgctgggca agctccgtac acgttcacgc atcgcagctt ctctgtgcgc ctaccgcgcg
     5941 gctacgatcc ccaaaaggcc tatcccctag tattccaagg tggaggctgc ggcaacaccg
     6001 acggcacgag cggtaagaat ggagcggaca agctcgttcc ggacgacgcg agcagcgagg
     6061 ccatcgccgt cggtcttagt tacgtctacc cggatggtgc gggggcgtgc ttcgcggatg
     6121 acggcgagaa cacttacgag ctgccctatt gggacgcagt ctacgccgcg gtcactgcga
     6181 gctactgtgt cgatctcgag aaggtcttca ttggtgggtt cagcagcggc gcgtggatgg
     6241 cttacaccgt cgcgttcgcg cgcgcgggct tggtgcgtgg catcggtacg ggcgccggag
     6301 gcatgcgcga gaaacacccc gcgcgcagca tcattccctt cgcggctttc atggtcactg
     6361 ggctcgatga cgacgccaac cccatccacc gcaccaagga cgggacgagc tgcacgggta
     6421 gcgaggccga cggctgctgg aagggcaaac tcatctgtgg cacgcccggc gctgagagct
     6481 gcttcgacac cggctccgcc cacgcgcgcg acgaaatcct aaagcgcaac ggctgcgtcg
     6541 gtaccgacac ggtccagcac ggtgaatggt ccgattgccg ccgctacacc ggttgccccg
     6601 cggcgttccc cgtcgtctat tgcaccccgc ccggcggcca cacgagcggg gacgaccgtc
     6661 ataacccggg cacctggaat ctctggaagg ctctgccggc ggtgccttag ctgtcacgtc
     6721 agcgcttcgg accaatcgct accccgttac tagccgtcta gcgacgaatc gggcgcctca
     6781 cgtcactccc tcgcgggacc gttgtctttg caatattgca gtgaagaatc gtgattcgac
     6841 agcacactcg acggaacggt gctcggaccg ccactgcgct gtgtgaagta cagatccgcc
     6901 gtcgttagcg ttgtaaatgt cacttccgaa tccaggcccg accgcgcgtc ggcgccctcg
     6961 agcgctcgtg gcgctttgcg acgggcagcg tcgctggctg gcacgaccgc gttcgtggcg
     7021 ttgagcgcta tcgcctgttc gtccagcacg actcctgata ccggagcggg cggtcaagtg
     7081 cccggaacgt ccggtggcac gagcggcacg ccagcgagtg gcggcgcggg agcaacgagc
     7141 gccggaggtc ggggtggcgc taccagctct caaactgggg gcagcactgc tacgggagga
     7201 agcgtcagca cgggtggcgc gaacgcttcc ggcggtagtt cgtctccaag tggcggggcg
     7261 actactggaa tgggcggcgg cgcagggctg agtggaggcg gcgccggcgg aacatccggc
     7321 gcaggcggtt cgtccggcgg cacggactcc aaaggtggca cttcgggttc gggagctgga
     7381 ggcggcaacg cgggagcagg cgctggcggc aagaccaccg gcgagtctgg gggagccggc
     7441 ggacgcggcg gtgcctccgg cgatggcgga acaggtggat cgaatggtgg agcgagtgga
     7501 ggctcgactt cttttcgacc atgtccggcg aacgatccgt gcaagatctt gccgctcggt
     7561 gattcgatca cccacggctt gcagtcgagt gacaacgctg gttaccgctc gcagttgttc
     7621 aagctcgcgg tcgcagacaa caaaaagctg accttcacgg gatcgcagag cagcggcccc
     7681 aatcaagtct ctggcgccac gttcccgaaa aaccatgaag gccacgacgg ctggaccgtc
     7741 gaccccggct tcagcaaata tggttcgggt gggatttcga gtctgattcc gagtcccgct
     7801 ctgtctacga tgccgaacat cgtactgctg atgatcggca ccaacgacat taccagcacg
     7861 gacaccccag gcgcgaccgc cgatcgactc gatggcttcc tcggtaagct cgtccaggcc
     7921 gcgcctgacg ccctcatcgt cgtcgcacag atcacgcccg tcgcctacga cagcgccgat
     7981 ctcaagaact acaactccaa gatcccgggc ctcgttcagg cgcgcgtggc gaaggggcag
     8041 cacctgattg gcgtcgatat gagcaagatg ccaacttccg gcctggcctc ggacaaagtc
     8101 cacccgaacg accaaggcta cgcctacatg gccaacgtct ggtacgaggc catcaaaggc
     8161 tacctgccga attgaggggg gggcgggagc cccgcacggt ttagcgtggg gctcccaatt
     8221 atcgaaccat ctcccccatc ccgcgccgct cgagcactcg cctcaactcg gggcgaagtg
     8281 gtttcacttt ggcgccctcc aaagcgagaa atgtgacgtt gtcttccgca agccggtaaa
     8341 cactgaatcc aggttgagtg ttgatttgtg agttgcgccc ggggccttgc ggatcccaaa
     8401 tgccaatgtg gaaccaaact ccgtccggat cggggttcgg gacttgtgca acccaaccgc
     8461 cgcggcgtgt cgaacggtga gtgcgatagg cgtaggagat ggtgcattcg ccgttaccgg
     8521 cgatcacgtt ccccggatcg aacgcggcaa gctggggata gttgggcttg agtgcgtcga
     8581 tatcgcgggc aatcgccgtg cagctcgcgg ttaaatcgtg gaggggctcg ctgccagtga
     8641 gggcaggggg gcgctcgagc gagcgtagga taatccggtt ccggtaggtt cccccggcgt
     8701 gcgcgacgct atcccagccc cgcccaatgc cgctcgcggc gagcgtgaag attagatgcg
     8761 cggggctctc gagattgggg ctctcgaggc caaactgaat cgcgttctcg ggaacaccat
     8821 atccagccgt gggcgagaga gcggtcgaga gctgactctc gaaatcgaga aactcgagtc
     8881 gcgcgaacgc accctcgggc tcctccatgt cgaccaagtc gacggcgagg gtcgcgtctc
     8941 ggggcaggct accctctgca agtgtcgtga acggcccttt ctccgcgtca gtggcttggg
     9001 caatatgaaa tctatgaccg gcgtccgcgt atgcgaatcg ggcgggcgcg ccgctcggat
     9061 agtcggcgtg ggtgacggcg atcagcgagt tcgcgcaagg ggagaagcgc aacccaagcc
     9121 gccgatgacc gctcaccatc acggtggtga acgagttcag gtgcgagtaa acggcgcgat
     9181 cgagccatgt ggaggactcg agttgcacga cctgagtggg tgcgttgggc aaagtgaagt
     9241 ctcgacggac cgcgagcatc ggtactccgt ccgctgtcca aagtcgaagt tcatcctttg
     9301 ctaccgttgc tgcggcgggc gatgctggcc cagtgaaagg cgcgaatatg gaccaaaagc
     9361 catcgggcga aatccgatcg aaggtgagca cgggcgaaat ggacacggcc gttcgtcggc
     9421 cacgccaggt cacgcgcgcc gccgggccct cgaccacgag atcgggtgcg agctcccaat
     9481 ccgcgagtat cggttcgcgg ggctcggtgg cgagccgtgg ttgccggccg ggccccgccc
     9541 gcgtgctggc cggcgcgggc ttctcgcaaa gagcggtgag cagaccgaat gcggccccgg
     9601 caaggagcgc aggaagcagc gccagcgctc gttctccgcg acaagcgaac cccgcgagga
     9661 tcgcgaagaa cagcccgctt agtaccaggg tgcgcgctgc ggtccaaccg gtctccggaa
     9721 aaatcagaat cagcccgagc gcaagcgaac accagaatgc aggaatcgcg ggcagccaac
     9781 gcgcggccgt gcgcggctca aacagcaaca gactcccaat cccgagcgag gttgcgacca
     9841 tcgcgaaagg cacgacgatt cccaacccgg tacgccagtg cagcagcggg aagccgtgcg
     9901 gcagcaacca ccaagcaagc ccacccaaga ccagatgagc aaggagtacg agcgcccaag
     9961 tgatccgatg cagggccgcg agggctcgag gcgaagacac gcccacagca tacccgcctc
    10021 tagaggatcg gtttagtctc gaccacccag atgatcgccg aacctttcat tcgagtgtaa
    10081 gttcgacgag ctggcgccgt tggaggccac atggctctgg tcaaaagaac cgctcacgtt
    10141 ctgactctgg ggttcaccgc gttctggttg gtggctactt cgcgggctcc ggggccggtg
    10201 cacccgtgct tcgtgccgct cgaggcgaag aaggtacgaa tagctctggg ggcggcgatg
    10261 ccggcctcac ccggcgcggc gccgagctgc caggggctgg acgatttgcg cgagggggcg
    10321 gtgattgggg tgggtctcat ccccagttca gatcgaaccg cacgcgtgcg cgtggaccaa
    10381 gacgatgctt gtttgggcta tgacgtgacg gaactcacgg ggccagttgg tctttcccag
    10441 cctcattcgg gtgatccgag gtacccggtg ccccgtgcac tattcgaatc gcgtgcgcac
    10501 ttcagcagcg ccagctcggc cccgcgctgt gctggcgagt atcacatttc ggttgcaccc
    10561 gtgaacccgc tgcctcggaa cgcactcgtc gatccgctcg acgccgggca ccgcgaacct
    10621 tggcaactcc gtcgcgtgat ccgcgtggac acaactcgcg tctgcgcgct ctctcccgct
    10681 cttccccccg gtgattgtca ggatatcttc gaggtgagga gccttgagct cgggccctga
    10741 agctagcgct ccgcgttccg gcgagttctt cgcgccgctc cccctcgcgg cgctggcctt
    10801 gctcgttgtg aacgacgtct acctgaaagc aaagttccac aacttcgtga cgggcaagtt
    10861 gagcgatctc gcggtctgct tcctgatgcc gctcttctta tcggagttgt tggggctcac
    10921 gctcgggatc gctccccgtc tgcgcctcac gatgggagcc gcaacaacgg ccgttctgtt
    10981 cagcgttctc gaagtcgtgc catcggcggc agcgtggagt gtgaacctac tcagcaggat
    11041 cgggccgcat ctcgggctcg gctgtcatta cgccatgacc gcggacatca ccgatctcgc
    11101 atgcgtccct gtcgttctgc tcgcctatgg atatggttgg aggcgagttc ttggcgtcgt
    11161 gagggcgcca gagctcagtg cgtagttccc gcggttccgc ccgcctcctc gcctccggcg
    11221 cccgcgaagc tcgcaccgcc gttctcgctt ccaccgtctc ctccggcccc ggctcctccg
    11281 gcggatccgc acgttcccca atcgattcga atctgaccgg ggggacagat gaagggttgt
    11341 ccacccgtgc accacgtccc gtcgctgcgc gcgacgcaga aggcatcatc caagtagcaa
    11401 tcagtctggt cctccacgat cttaccgggc aagcccgaag ggccaacaca gtcggagtct
    11461 gttccgccgt gggttcctcc gccgctcagc ccggccgtgt tcgaggtgtc ccctcctgcg
    11521 ccgtccactc cgcccgctcc gccattgacc atatcgggaa tgcaagccgc accgggcaaa
    11581 ctgcgatgcg tcccaggcgg acagggctgg ggaatgagac cgcccgtgca aaaggttcca
    11641 tcgcccaagt cttcgcagaa tgcatcatcg cgaaagcaat cgttccgact gcccaccacg
    11701 acgcccgagc cagagcggca ggaatcgccc acgcgatgtc ccgtagttcc cgcggtccca
    11761 gcggtcgatc ctccggagcc acctgcaccg cctttatttc cggaaccgcc cgcaccgccc
    11821 ttggtcgtgg cgccacccgt acccccgttg ccagggtccg actcaccaca gccaatcgct
    11881 agccacaacc acaggacagg cgccaagctg agccggccta agcttccgaa ttggcacgca
    11941 ttgacacaaa atcttcgaga acgagtcgtg cccatggtca ggggcaactc gtgtgccatg
    12001 ccctacgtta gaccaggctg tgaggttcgg ccgacttcga gctcagccgt caccaggtgg
    12061 ccgtcttttt cgcggccgga gtcctcagcc aggccgcgaa acgcaatttc caatcttgga
    12121 gagcaatcca ttcctgatcg cgcttttcat cgaacacgat ccaccccgcg cactcgtgtg
    12181 aaagctgttt gagtcgatcg atgtgttcgg ctctgaatga cgagcgcccg taggcgcggc
    12241 gctcccccag cacgacctgc cacaacgtat actcgagatt gtccatccat cccgcgcaat
    12301 acgcttcttc cgagagctcg gacatgtact cggccaatgc gcgctgaggc ggggtaagat
    12361 ctctcaacat cgtggtgcgg gcagcgtaac gacactccgc gctgggcgct agggcatatc
    12421 ctcccgcagc tgacctcgaa agactttgaa gcaatgcctc gagaacgagc gcaactgcct
    12481 gttatgttcc gtggcggcta ccgccaggtg agcaaacttg gccgagcgcg gcgggctggg
    12541 tgatcgtggg aacgatgatc agcacgcccg acaaggcgac cgcgcgtgac cagctgctga
    12601 cgcagctcgg tgaagaagca tccacgaagc tacgcctcgc gaccgtgagg ctcacggctg
    12661 ggttattcac cgggagcggc gtgatcctca cgaccgtgat caaaggtgat agaggcgtgt
    12721 tcgtggttgc caccgcagcc cacaatcttt ggttctatgt ggactctctg acgggcggca
    12781 cgagccgcga cttttggggc ggagacaagg gcaagtggtc ggcagaggtg ctgagtagca
    12841 agaaacaggc gatcatcgac ctatgcctcg cgcggctgaa cgtggagtac gccagcgcgg
    12901 atatgaccca gccaaacccg accgccgccg tgaccaccgc cacgatcggg cgcgtcatac
    12961 tggctgagtt ccagtacgat ctgtgtctgt tgttgatccc gttccccctg cgggcgcgtc
    13021 cccgttgcga gatcgtcgcc
//
LOCUS       DASTKU010000003         4669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_892553, whole genome shotgun sequence.
ACCESSION   DASTKU010000003 DASTKU010000000
VERSION     DASTKU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892553"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..1942
                     /locus_tag="VFQ61_00075"
     CDS             365..1942
                     /locus_tag="VFQ61_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952861.1"
                     /translation="MASLEGIEAGTRLGPYRVEKRLAAEPRAFFQAINTDAPERPVCL
                     LELRDDEVEAYASAAQRSTQHAHLARVLEVLREEGHALLVFEDAVGTTLTERLAERGK
                     KPEVEAVGSALRIADALSTLHEAGCVHGFVHTDSVIVDPPERLGPVLKFAPIPAEERT
                     FHSPERGETGKPSVADDAWAVAGLLHMMLTGNPPPKDGYAEKQELKAAGVSHEALAEA
                     LLHGLHRDAKVRSSDVRPLKRELARWFVEHAGEEPVLVGPHSTAPPPLPELPLQVMAA
                     RAPLRVKRRPRFLMLATVGTLIGLVGAWGISALLAKREVRAQRPSSVQAAPEPVATGG
                     VTPVSASAAGPKEIDLSEVPVTGEEQKHVELDKASSCVAGYLPKGAFGKSPDLTWLCE
                     ESNPAKGAEKLRAAVVSSAPRGVVTDAMKLFARLGWYDMAAFAVVRAGCCEDVKPLRM
                     HDDVGKSCTDKMPQALDEIAKEVIGGGNRAEALKRFNSSIQCEINQSHSLQIGRAQRF
                     DPNEEAVFGEFVEAIAH"
     gene            complement(2076..4466)
                     /locus_tag="VFQ61_00080"
     CDS             complement(2076..4466)
                     /locus_tag="VFQ61_00080"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HET9952862.1"
                     /translation="MVPSWALAQPHQGGEASLVLPDLSSVEVLGTNGHTLLLLGLLVA
                     FAGLAFGIVALLQVKNMPSHRSMSEISAIIWETCKTYLITQGKFIMMLEVLIGAIIVL
                     YFGFLQHLGAARVLIILIFSIIGILGSYGVAWFGIRINTYANSRTAFASLSGKPHSVY
                     MIPLRAGMSIGMLLISVELILMLAILLFVPGDYAGPCFIGFAIGESLGASALRIAGGI
                     FTKIADIGSDLMKIVFKVKEDDARNPGVIADCTGDNAGDSVGPSADGFETYGVTGVAL
                     ISFILLAVRDPATQVLLLVWIFSMRVIMVVASAGSYLFNEVLQKGLIEKATKVNFEAP
                     LTWLVWLTSLVSVALTFVVSYLLIPNLGGDDTLWWKLSAIISCGTLAGAIIPELIKVF
                     TSTESGHVSEIVTASREGGASLNILSGLTAGNFSSFWMGLVVAVLMAVAYAISGMGIE
                     TLMSAPAVFAFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVFELSTITEIEGAAEEIE
                     KEHKFKVDFEAAKRLLEENDGAGNTFKATAKPVLIGTAVVGATTMIFSIVVLLTHGLT
                     ENLDRLSILHAPFLLGLIAGGAVIFWFTGASTQAVVAGAYRAVEFIKRNMKLEGATKA
                     SIEDSKKVVEICTRYAQKGMVNIFMTIFCGTLAFACIDPFFFIGYLISLALFGLFQAL
                     FLANAGGAWDNAKKVVEVELADKGSDLHAACVVGDTVGDPFKDTSSVAMNPIIKFTTL
                     FGLLAVELAIQLDRSTSSALAALFGGAMLFFVWRSFYGMRIGVADEGARAHAPAAE"
BASE COUNT          938 a         1461 c         1461 g          809 t
ORIGIN      
        1 cttcaacagc atgccgcgaa ggtacgtact acctggccct actgtcgcaa acttttctgt
       61 ctcggctcat ccaatccctg ctgcttcttt atccggactt gccggcgctg cagcgccgcc
      121 cgagaggctc tgcagcgccg cccgagagcc cgtgccggcc tcagaccgga cacgctcatc
      181 accgatgccg gcacggacgc cggccatccg gctcggatcc agtccttctg gtttccagtc
      241 tggtgtttct gaattccagt aagccgtgct tcgtcgtgcg tgcggaaacc tcccaccgca
      301 cgcataaaac ttccctgaat ctggacgctt gcatgacaaa gtgcaagtct ccgtaacgag
      361 gtcagtggct tcgctcgagg gaattgaagc aggtactcgg ctcggcccat accgagtcga
      421 aaaacggcta gcggctgagc cgcgagcttt ctttcaggcg atcaacacgg acgctcccga
      481 gcgaccagtg tgtctgctcg agctgcgtga cgacgaggtc gaggcgtacg cctctgcagc
      541 gcaacgcagc actcagcatg cgcacctggc gcgtgtcctc gaggtgctgc gcgaggaggg
      601 gcacgccctt ctagtattcg aggacgcggt cggcaccacg ctcacggagc gcctggccga
      661 acgtgggaag aagccggagg tcgaagcggt gggctccgcg ctccgcatcg cagacgcgct
      721 gtctacgttg cacgaggcgg gctgcgttca cggtttcgtc cacaccgata gcgtgatcgt
      781 cgatccaccc gagcggctcg ggccggtctt gaaattcgca cccattccgg ctgaagaacg
      841 caccttccac agcccggagc gtggagaaac cggcaagccc tcggtggctg acgatgcctg
      901 ggccgttgcc gggctgctgc acatgatgtt gacgggcaac ccgccgccca aagacggcta
      961 cgccgagaag caagagctga aagcggccgg agtttcccat gaggccctgg ccgaggcgct
     1021 gctgcacggg ctgcatcgcg atgccaaggt gcgcagctcg gatgtgcgcc cgctgaagcg
     1081 cgagctggca cgttggttcg tggagcatgc gggcgaagaa ccggtgctcg tggggcccca
     1141 ttcgaccgcg ccgccccctc tgccggaact acccctgcaa gtcatggccg cgcgcgcacc
     1201 gttgcgggtc aagcggcggc cgcgcttcct gatgctcgcg accgtgggca cgctgatcgg
     1261 cttggtgggc gcgtggggga tctctgcctt gctggccaag cgggaagtgc gcgcccagcg
     1321 tccctcgagc gtgcaggccg cccccgaacc cgtggcgacc ggcggcgtca cccccgtgag
     1381 tgctagcgcg gccgggccca aagagatcga cctttccgag gtgccggtca ccggcgaaga
     1441 gcagaagcac gtggagctgg ataaagcgag ctcctgcgtg gccggctact tgcccaaggg
     1501 ggcgttcggc aagtcgcccg atttgacgtg gttatgtgaa gaatcgaacc cggccaaagg
     1561 cgccgagaag ttgcgtgcgg cggtcgtgag cagcgcacca aggggcgtgg taacggacgc
     1621 gatgaagctg tttgcgcggc tcggttggta cgacatggcc gcgttcgccg tcgtgcgcgc
     1681 cggctgctgc gaggacgtca agccgctacg catgcacgac gatgtcggca agagctgcac
     1741 ggacaagatg ccgcaagcct tggacgagat tgccaaagag gtgatcggcg gtggtaaccg
     1801 agcggaagcc ctgaagcgct tcaactcgag catccaatgc gagatcaatc agagccatag
     1861 cctgcagatt ggccgcgcac agcgcttcga tcccaacgaa gaagccgtgt ttggcgagtt
     1921 cgtggaagcc atcgcgcact aggcggcgtc ggctggcagg ggtcgtttcg gcagggccca
     1981 tcgaaacgag acctcgagcg ttatcgcccc acacgtaaag ccaggcacgg ccgttgaggc
     2041 gcggtccgcg ccccaacggc ttccgttccg cggttctatt cagcagccgg agcgtgggcg
     2101 cgcgcgccct cgtccgcaac gccgatgcgc atgccgtaga acgagcgcca cacgaagaac
     2161 aacatcgccc caccgaacaa cgcggcgagc gccgagctcg tggagcgatc cagctgaatg
     2221 gccagctcta ccgccaacag cccgaacagg gtcgtgaact tgatgatggg attcatggcc
     2281 acgctggagg tgtccttgaa agggtccccg accgtatcgc ccaccacgca ggccgcgtgc
     2341 aaatccgagc ctttgtctgc gagttcgacc tcgaccacct tcttggcgtt gtcccaagcg
     2401 ccaccggcgt tggccaagaa cagcgcttgg aaaagcccga acagcgcgag cgagatcaag
     2461 taaccgatga agaagaacgg atcgatgcag gcgaacgcga gcgtgccgca gaagatggtc
     2521 atgaagatgt tgaccatgcc cttttgggcg tagcgcgtgc agatctccac caccttcttg
     2581 ctgtcctcga tcgaggcctt ggtggcgcct tcgagcttca tgttgcgctt gatgaactcg
     2641 accgcgcggt acgcacccgc caccacggct tgagtggagg cgcccgtgaa ccagaagatc
     2701 accgcgccac ctgcgatgag ccccagcagg aacggggcgt ggaggatgct caagcggtcg
     2761 aggttttcgg tgaggccgtg cgtgagcaac accacgatcg agaagatcat ggtggtggcg
     2821 cctacgactg cggtgccgat tagcacgggc ttggcggtgg ctttgaaggt gttgcccgcg
     2881 ccatcgttct cttcgagcag ccgcttggcg gcctcgaagt ctaccttgaa tttgtgctcc
     2941 ttctcgattt cttcggctgc tccctcgatc tcggtgatgg ttgagagctc gaacacactt
     3001 tgcgcgttgt ccgtcacggg gccgtacgag tcgacggcga tcgttaccgg tcccatgccc
     3061 aagaagccga acgccaccag gccgaacgcg aatacggcag gggcgctcat cagcgtttcg
     3121 atccccatgc cgctgatcgc gtacgccacg gccatcaaca ccgcgaccac caagcccatc
     3181 cagaagctgc tgaagttgcc cgcggtgaga ccggacaaga tgttgagcga ggcgccgccc
     3241 tctcgagagg cggttacgat ctcggacacg tgcccggact cggtcgaggt gaagaccttg
     3301 atcagctccg ggatgatggc acccgccaac gttccgcagc tgataatcgc cgagagtttc
     3361 caccacagcg tgtcgtctcc gccgaggttc gggatgagta gataggaaac tacgaatgtc
     3421 aacgcgaccg agaccaggga ggtgagccac accaaccagg tcagcggcgc ctcgaagttc
     3481 accttggtgg ccttctcgat caagcccttt tgaagcacct cgttgaacag gtaggagccg
     3541 gcgctcgcga cgaccatgat cacgcgcatc gagaagatcc acaccagcag aagcacttgg
     3601 gtggcggggt cccggaccgc gagcagaatg aacgaaatca aggcgacacc ggtgacgccg
     3661 tacgtctcga agccgtccgc gctgggaccg accgagtcgc ccgcgttgtc tcccgtgcag
     3721 tcggcaatca cgccgggatt gcgcgcgtcg tcttctttga ccttgaacac gatcttcatc
     3781 agatcggagc cgatgtcggc gatcttcgta aaaatgccgc cggcgatacg cagcgcggag
     3841 gcgcccagag actccccgat cgcgaaaccg atgaagcagg gtcccgcgta gtcaccgggg
     3901 acgaacagca aaatcgcgag catcaggatc agctccacgc tgatcagcag cattccgatg
     3961 ctcatcccgg cgcgcagcgg gatcatgtag acggagtgtg gcttgcccga gaggctcgcg
     4021 aacgccgtgc gcgagttggc gtacgtgttg atgcggatgc cgaaccaagc gacaccgtag
     4081 ctgcccagaa tgccgatgat gctgaaaatt agaatgatca gcactcgcgc cgcgcccaag
     4141 tgctgcagga agccgaaata cagcacgatg atcgcgccaa tcagcacctc caacatcatg
     4201 atgaacttgc cctgagtgat caggtaagtc ttgcaagtct cccagatgat tgcggagatc
     4261 tcgctcatcg agcggtgaga aggcatgttc ttcacctgca atagagcgac gatgccaaag
     4321 gccaagccag caaacgctac gagcaggccc aacagcaaca acgtgtggcc attcgtaccg
     4381 agtacttcga cactcgatag atccggcaat accaagctcg cttcaccgcc ttgatgcggt
     4441 tgcgcgagcg cccaagatgg aaccatgagc gtcagccacc cacacagggc agcgaccagt
     4501 ttgctgatgg atgagcctga tcggtacaag gtgtactcct taccgcgctg cggacgccgc
     4561 acgcttttca acgccagtga gcttttactg tccccggcag acttgcgcct gaagtgagca
     4621 aatgtgcttg cgagggagcc gagcgagtct cgccggcaat ctcgcttag
//
LOCUS       DASTKU010000004        13839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1561643, whole genome shotgun sequence.
ACCESSION   DASTKU010000004 DASTKU010000000
VERSION     DASTKU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13839
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561643"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFQ61_00085"
     CDS             <1..535
                     /locus_tag="VFQ61_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HET9952863.1"
                     /translation="AALPPGLPPVVIVQHMPAYITKPFADRLQQLSRLRVDEARDGEL
                     LEPGNVRLAEGGKHLLVERVGGQLRLRVNEGPRVNGHRPSVDVLFQSTAQAVKNRAIG
                     VLLTGMGRDGAQGLLTMRRAGAYTFAQDEASSAIFGMPRAAIELGAVSEVVAPRHLPT
                     RLIAALSTRSPAASAAR"
     gene            complement(833..2794)
                     /locus_tag="VFQ61_00090"
     CDS             complement(833..2794)
                     /locus_tag="VFQ61_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET9952864.1"
                     /translation="MPIIQAHGVSKTYGTRSVLRDVELTIRTRERVGMVGSNGAGKST
                     LARILAGIEAPDTGNVSRRRGASVVYLSQTPKFEQEISAEETLLEALTAWRDAVARHE
                     AATRAVTTTSGAELDTWLEAQHRAAEDVERLGGWDQRHRAVALLGHVGIQDSKALVTR
                     MSGGEQRRVALARMLLQNPDLAILDEPTNHLDAETIEWLEQYLIEEHTGAVLLITHDR
                     YLLDRVAERTFEINDGQVFSYDGGYELYLEQKAERDAQAARTEQNRQNFLRRELEWLQ
                     RQPKARTTKQKARIERAEAAKQAIAPKREANLELELETVRAGKTILELRELSLDMPGE
                     GPRRPLIRNLDLFLARGEIIGIVGPNGSGKTSLLRAILGELSPQSGRIVVGQNTKIAY
                     FDQNRTGLKEEASIYENVSEDQSTIELGGTALDPRSYLERFNFDRYAQRQPVGSLSGG
                     ERARVALAKLLRQPSNLLLMDEPTNDLDVRTLGALESMLLDAGATALVVTHDRWFLDR
                     IASRILAFENDARVVVYPGNYSSFARLRREAREAHEAVQKQQAAALAAETAKKEAERK
                     KVAGPTEAPPAKPKPLNRAERDELAQLPDRIERAEQLVTELTHQLSDPAAHSGGGSRA
                     AEILKQLDATKLEVERLMARWEELETRKS"
     gene            complement(2860..3705)
                     /locus_tag="VFQ61_00095"
     CDS             complement(2860..3705)
                     /locus_tag="VFQ61_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET9952865.1"
                     /translation="MRNLRYVLCDVFTSEPLKGNPLAVFTDARGLDAQTMQDLAREMN
                     LSESVFLLKPEAGGHARLRIFTPKREIPFAGHPTLGTAFVLGGPLSVESVQLETGVGT
                     ISVRLEREAAKIVFAWMTQRVPTAQPFERADELCRALGVARSELPIIEYDNGMRHVFV
                     VLDSEQAVARVQPDFAGIAELTSAGVNVCAGSGTQFKTRMFHPTAGVNEDPATGSAAG
                     PLLVHMTRHGRAPIGEPVRIAQGAELSRPSELFATVFGSTDQIERVEVGGAAVILGRG
                     ELRIP"
     gene            3956..5011
                     /gene="ruvB"
                     /locus_tag="VFQ61_00100"
     CDS             3956..5011
                     /gene="ruvB"
                     /locus_tag="VFQ61_00100"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET9952866.1"
                     /translation="MAKKKSTSVEAEDTRLLAPAAQADEDRFDRIFRPETLDDFIGQE
                     KHKDNLRVYVQAAKRRREPLDHILLCGPPGLGKTTLANILAHEMGVKLYVTSGPVIEH
                     KGMLTGILTRLERGDILFIDEIHRLSPAVEEFLYPAIEDFRIDVATGDGAFAESIALD
                     IRPFTLVGATTRTGLLTKPLQERFGVTLRLDFYPPEELEQIALRSAKLLGIPCDVQGA
                     RELASRSRGTPRVANRLVRRVRDFAEIEGNGRMDRAIVELTCQRLGVDAAGLDEMDRR
                     LLSAIVLHYDGGPVGVETLAAALAEARDTVEDVYEPYLLQQGFLGRTPRGRVATRKAY
                     EHLGVPFHGETRQGSLF"
     gene            5008..5823
                     /locus_tag="VFQ61_00105"
     CDS             5008..5823
                     /locus_tag="VFQ61_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9952867.1"
                     /translation="MSGNADRYPVRPGAEAYRAVARPGAPALRARTGVALIHGFTGSP
                     VSMRPLAELLHELGFSVDVPRLPGHGTHPRDMARTRYADWRREVLDTIDRLSRVTERT
                     VLVGLSMGGSLVLDVASSNERTLAGVVVINPQLLDRGGAAVKMAPLVERVLPFVPGAL
                     GGLRKDDIAKPGVSEQAYDWFPTAAGNSFMAELPRIREQLNHLSCPLLVAHSMRDHSV
                     PPANSEALLKALPHARELRLERSYHVATLDYDLELLAEKICEFCENPAPAKHA"
     gene            5820..7580
                     /locus_tag="VFQ61_00110"
     CDS             5820..7580
                     /locus_tag="VFQ61_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952868.1"
                     /translation="MRYRAVAVASAESNAVGAVELECTPHGLFIAYLGVGAFTEGYAP
                     GALASGTGLTVPWTQVSEARLEGERLLLAIDHKLTPLNRLLLTEFETGRRIPKEELAK
                     RRLLVRFSSLAAALVAALILGAFVLRSSPETSASAAVALALLAALVLMTMGFLVDRSM
                     ESSAEDRAAFENFAREISQYLPTLVQLPHAPRARKPLPDVSELQGLLPRTTLAIVITL
                     TSGLLSVLLVARWVTNNQAEVRSVTSRMLEPAAPREAAAAARPQAAPKAPKPSSPPQV
                     AAARDVAATPASSGPGQGFGDECRCVRPDSALWSRPLPRLSVLTISKRLRLGKNPDEN
                     KRKKYLELDLAVVNNSNKDIEEIALMVLFYDRDPPPSNRRNQVSNRPLFFEGPLQPGQ
                     AIKWSVEAEGTEFEVQNPIAGSLTPEGDDIAPANRLAELLSARNRPVRLHGAMLLTFL
                     SDPRAREGALGLREALREDEAPYLSRLIQALGDVRVCQLRVSSGPGPARTVNACVHNT
                     SKEPKKELGMKLRGLENAPDLGNPSADPPTVQFETTVHLPGELGAGAARTVRFPVDLG
                     SAAPTIYEAYVDRFDLLASP"
     gene            7593..8078
                     /locus_tag="VFQ61_00115"
     CDS             7593..8078
                     /locus_tag="VFQ61_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9952869.1"
                     /translation="MAALMTAETLAIRPARPGDQHAIFDLIRELARFERLEHVVSGSP
                     DALAHDLFGERPSAEALLAESDGVPIGFALFFSTYSTFLTRPGLYLEDLFVLEPYRGQ
                     GVGRALLRAVHERARERGAGRLEWSVLDWNERAIAFYRSFGATVLPDWRICRIELSPT
                     T"
     gene            complement(8088..11411)
                     /locus_tag="VFQ61_00120"
     CDS             complement(8088..11411)
                     /locus_tag="VFQ61_00120"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HET9952870.1"
                     /translation="MTEPHVLIAAILQSEPQARIGVLERFADHASLAELTRAARELHE
                     FASQPEHNLYQRVRAIFQAHALYRYWIPARPELRRAGRIPRQGHELLLARHYAAATDA
                     FLAEADQHGLNDPLSSALAAAYHGLGFQLLSEQVQRSVRSLRGNRWMFRAGHALDQPL
                     RVHERLKQPDPAGFYPVLVERTPVRMDLTHSGWSDIFFLGMDYPEGARVLNVSVDLAV
                     HGRDLRTAPPISCYLRVIDEPVLRLVSVDLDAAADLTETSEVFNYASDYLGLLKAAVI
                     ASGIVPPALEGSEQPLSLLLEQLVGPGRGLELASQVNDIPKGSRLAVSTNLLGGLISV
                     CMRATGQISSLTGGLTEAERRMVAARAILGEWLGGSGGGWQDSGGVWPGIKLIEGVPA
                     AEGDVEWNSSRGRLLPRHTLLGEDRVSPAAQRRLEESLVVVHGGMAANVGPILEMVTE
                     KFLLGTEREWHARQELLSAFEQVVAALAQGDMRELGRLTTRLFEGPLATIIPWVSNFY
                     TEQLIADARAAFGEKFWGFWMLGGMSGGGMGFIFDPSVKAEAEVRLLEIMRRRKRELA
                     ASLPFAMDPVVYRFSVNGQGSSAELLSGEAATFSAEYYLCMLPRWLRDGSRSFSPQRR
                     AEIDAFSRQHLRGERAERVGQALLGRLLPATQSREAEESLQALLDRYGFDAEQHERIR
                     ADLRAERIGLAKNRLPADTSVEDVHPKDVVHAAALGPRELDAGSAALARGEVCVITLA
                     AGAGTRWTHGAGTVKAIYPFAKLGNRFRTFIEVHLAKTRKRGREFGVIPPHVLTTSYL
                     THAPIAGLIERENAYGLPESVHLSEGRSIGLRLVPTAHDLRFAWAELREQKLEERKQK
                     VRDAGRAALIEWAIRTGEASDYRDNVPSQCLHPVGHWYEVANLLLNGTLRRLLRARPE
                     LKYAMLHNIDTLGADLDPRWLGRHVESGAAFSFEVVERRLEDRGGGLARVGGRTRLVE
                     GLAFPREEDEAKLTYYNSLTTFISLDRLLELFGLNRAALEDEKAVLRGVRSLAQRLPT
                     YVTLKEVKRRWGNAQEDVFPVTQFEKLWGDMTALPDVKTEFFCVPRPRGQQLKDVAQL
                     DGWLRDGSKDYVESLCLFE"
     gene            complement(11414..12268)
                     /locus_tag="VFQ61_00125"
     CDS             complement(11414..12268)
                     /locus_tag="VFQ61_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9952871.1"
                     /translation="MRIKKAIIPMGRPVHADLPLQHVTTADGGTKRVAALQIEELLEA
                     GISQVALVTRPGGDRVFGELRGQFGNALVSIEQLEPRGFGDAVLCAESWVAGEPFVVQ
                     VCDHVFVTHAKASCTRQLVEAAEREGCAVSGVQVNAENQLPYFGVVGGNRVKGASRLY
                     SVQTVIEKPTPTVAEERCMIAGLRQGTYLALFGTHALSPTVFQVLREQRDGLAPDGKL
                     GLTEALDLLATREKYLALEVDGQRVDLESPFGLLRAQLALSLYGARREEVLRLILEEV
                     ALPPSERG"
     gene            13098..>13839
                     /locus_tag="VFQ61_00130"
     CDS             13098..>13839
                     /locus_tag="VFQ61_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952872.1"
                     /translation="MAQSRDRRRIDWRIIMDGKLPAARLSNPTLPVKQLAALIAEAMS
                     DPTPETPDRIIRHTVDFSKETLQLERSAVYLLDEENFLMTGTWGSDAQGSTIDEHALI
                     YDYGPIDREVFARAQAGFPWTAYDDCPHIAQVGEETQVIGQGWVACTAIVGPTGPLGI
                     FFNDTAISHNPLDESRQWRAAILCSILANALWRLKTADVSTTAHRETPQPEIVRRVAR
                     LLTQDPTLSCDALARQLNLSPRGLARSFK"
BASE COUNT         2429 a         4594 c         4411 g         2405 t
ORIGIN      
        1 cgccgcgctc ccgccgggcc tacccccagt cgtgattgtc cagcacatgc cggcgtacat
       61 caccaagcct tttgcagatc gattgcagca actcagccgc ctgcgggtcg acgaggcgcg
      121 tgatggcgag ttgctcgaac ccggaaacgt gcggctcgcc gaaggcggca agcacctgtt
      181 ggtcgagcga gtaggcgggc agcttcgcct ccgtgtcaac gaaggccccc gcgtcaatgg
      241 ccaccgcccc tcggtggacg tgctcttcca gtccaccgca caggccgtga agaatcgcgc
      301 gattggcgtg ctgctcaccg gcatgggtcg agacggagcg caaggcctac tcaccatgcg
      361 ccgagcgggt gcctacacct ttgcccaaga cgaagcctct tccgccatct tcggcatgcc
      421 gcgcgccgca atcgagctcg gcgccgtcag cgaagtcgtg gccccgcgtc acctccccac
      481 ccgcctgatt gccgcgctct cgacccgttc gcccgccgcg agcgccgcgc gctgagacgc
      541 acgcgggctc ctcgagcttg gccggtccgt gcgcgccacg agacaacgtg cgcgccacgc
      601 ggcgcgggcc gcccgaagcg cagtctcgag cccgcgcggg ctccccctgc accgcgccgg
      661 cgctcgcgtc gaccccgacg tccaagtaac cccgacgtcg cgttgactcg tgcggcccgc
      721 gcggtggcac gcggcgccgg atggcactcg gctgcgccaa ggagcccgct cgacgagcag
      781 cgcgcacgtt cttgcacggc cgcacattca caaaatattt tctttcgcga gctcacgatt
      841 tacgtgtttc gagctcttcc cagcgagcca tcaagcgctc gacttcgagt ttggtggcgt
      901 ccagttgttt gaggatttcg gccgcacgcg agcccccccc gctgtgcgcg gccgggtcgg
      961 agagttggtg ggtgagctcg gtgacgagct gttcggcgcg ctcgatgcga tcgggcaact
     1021 gcgcgagttc gtcgcgttcg gctcgattga gcggcttggg cttggccggt ggagcctccg
     1081 tcgggcccgc gaccttcttg cgttcggctt ctttcttggc cgtctcggcc gcgagcgcgg
     1141 cggcttgctg cttctggacg gcctcgtgcg cctcgcgggc ttcgcgacgc aggcgcgcga
     1201 acgagctgta gttgccgggg tagaccacga cccgcgcgtc gttctcgaac gccaggatgc
     1261 ggctcgcgat gcgatcgaga aaccagcgat cgtgggtcac gacgagggca gtcgcacccg
     1321 catcgagcag catgctctcg agcgcgccga gcgtgcgcac gtcgagatcg ttggtgggtt
     1381 cgtccatcaa caacaggttc gagggctggc gcagcaactt ggcgagcgcc acacgcgcgc
     1441 gttcaccgcc ggagagggag cctacgggtt gacgctgggc ataacgatcg aagttgaagc
     1501 gctcgagata gctgcgcgga tcgagcgcgg tgccgccgag ttcgatcgtg gactgatctt
     1561 cggagacgtt ctcgtaaatc gaagcttcct ctttgagtcc ggtgcggttc tgatcgaaat
     1621 aggcaatctt ggtgttctga cccaccacta tgcggccgct ttgggggctg agctcgccga
     1681 gaatcgcgcg cagcaggctg gttttgccgg agccgttggg gcctacgatg ccgatgatct
     1741 caccgcgcgc gaggaacaga tccagattcc gaatcagcgg tcggcgcggt ccctccccgg
     1801 gcatgtcgag cgatagctcg cgcaattcca agatcgtttt gcccgcccgc accgtctcga
     1861 gctcgagctc gaggttcgct tcgcgcttgg gagcgatggc ttgcttggcg gcctcggcgc
     1921 gctcgatacg cgctttctgt ttggtggtgc gagctttggg ttggcgctgc agccattcta
     1981 gctcgcggcg caggaagttt tgtcggtttt gctcggtacg agcggcctgt gcatctcgct
     2041 ccgccttttg ctcgaggtac agctcgtagc cgccgtcgta cgagaagacc tgcccgtcgt
     2101 tgatctcgaa ggtgcgctcg gccacgcgat cgagcaagta ccggtcgtgc gtgatcagga
     2161 gcacggcgcc ggtgtgctcc tcgatcaggt attgctcgag ccactcgatg gtctccgcgt
     2221 ccaagtggtt ggtcggctcg tcgaggatgg ccagatccgg gttttgtagg agcatgcgcg
     2281 ccaaggcgac gcgacgttgc tccccgccgc tcatgcgggt gacgagggct ttcgaatctt
     2341 ggatgcccac atgcccgagc agcgctacgg cgcgatggcg ttggtcccag ccgccgaggc
     2401 gctccacgtc ttccgcagcg cgatgctgcg cttcgagcca agtgtcgagc tcggcgccgc
     2461 tggtggtggt gaccgctcgg gtggccgctt cgtggcgcgc caccgcgtcg cgccacgccg
     2521 tgagcgcctc gagcagcgtc tcttctgcgc tgatctcctg ctcgaacttc ggggtttggg
     2581 acaggtacac gacgctcgcg ccccgccgcc gggagacgtt gccggtgtcg ggagcttcga
     2641 tgccggccag gatgcgcgcc agcgtgctct tgccggcgcc gttcgagccc accatcccca
     2701 cacgctcgcg cgtgcggatg gtgagttcta catcgcggag cacgctgcgc gtaccgtagg
     2761 tcttggagac gccgtgggct tgtatgatgg gcacgtggct ttaccgctgg agggagggct
     2821 ctggggggcg gagatttcga gaaggagcgc gaagccgagt cacgggatgc gcagctcgcc
     2881 gcgaccgagg atcacggcgg ccccgccgac ctcgacccgc tcgatctggt ccgtgctccc
     2941 gaacaccgtg gcgaagagct cagaggggcg gctgagttcg gcgccctgcg cgatgcgaac
     3001 gggttcgccg atcggggcgc ggccatggcg cgtcatgtgc acgaggagtg gtcccgccgc
     3061 ggatcccgtc gccggatctt cgtttacgcc cgcagtgggg tggaacatgc gtgtcttgaa
     3121 ctgcgttccc gagcccgcgc aaacgttcac gcccgcgctc gtcagctcgg cgatgcccgc
     3181 aaagtccggc tgcacacgag ccacggcttg ctccgaatcc agcaccacga acacgtgccg
     3241 cattccgttg tcgtactcga tgatgggcag ctccgagcgg gcgaccccga gcgcacggca
     3301 cagttcgtcg gcgcgctcga acggctgtgc cgtaggcacc cgctgcgtca tccaagcaaa
     3361 tacgatcttt gctgcctccc gctcgagccg caccgagatt gttcccacac ccgtttccaa
     3421 ctgcacggac tcgaccgaga gaggcccgcc caagacgaac gcggtgccga gcgtcggatg
     3481 gccagcgaac gggatctcac gcttcggagt gaagatgcgc aaacgggcgt gtccacccgc
     3541 ctcgggtttg agcaagaaca cggactcgga gagattcatc tcacgcgcga gatcctgcat
     3601 ggtctgcgcg tccaacccgc gcgcatccgt gaagacggcc agcggattgc ccttcagggg
     3661 ttccgaagtg aacacatcgc acaatacgta tcgcaagttg cgcatggcag ccgggcagct
     3721 ctagagcaac aagaggctcg gggccagtgc cgaacacgac cgagccggct cagtctcggc
     3781 cactttggcc gttcgtctcc aaggcgtcgc tgcttcaggt cggaatcggg cgcgggaacg
     3841 acgctcccgc atgtcggaaa acccgcgatt tcgcgcgaat ttgcgggccg cggccaacac
     3901 ccggcccata agcacaagca ttgcgcccgg gtctgcgata aaacgccgca cgagcatggc
     3961 gaagaagaag agcaccagtg tcgaggccga agacacgcgc ctgttggccc ctgccgcgca
     4021 ggcagacgag gaccggttcg atcggatctt tcgacccgag acgctggacg acttcatcgg
     4081 tcaggagaag cacaaagaca acttgcgcgt ttacgtgcag gccgccaagc gccgccggga
     4141 acccctcgac cacatcttgc tgtgtggccc gcctgggctc ggcaagacca cgcttgccaa
     4201 catcttggcc cacgaaatgg gcgtgaagct gtacgtcacg agcgggccgg tgatcgagca
     4261 caagggcatg ttgaccggca tcctcacgcg gctcgagcgg ggagatatct tgttcatcga
     4321 cgagatccat cgcctgagcc ccgccgtcga ggagttcttg tatccggcga tcgaggattt
     4381 ccggatcgat gtcgccaccg gcgatggcgc gttcgcagag agcatcgcgc tcgatattcg
     4441 cccttttacc ctggtcggcg cgaccacgcg cacggggctc ttgaccaagc cgctccagga
     4501 acggttcggg gtaacgctgc gtctcgactt ctacccgccc gaagagctgg agcagatcgc
     4561 gctgcgcagc gcgaagttgc tggggatccc gtgtgacgtg caaggcgcgc gggagctcgc
     4621 cagtcgatcc cggggcacgc cgcgcgtggc caaccgcttg gtgcggcggg tgcgcgactt
     4681 cgcggagatc gagggcaacg ggcggatgga tcgagcaatc gtcgagctca cttgtcaacg
     4741 tttgggcgtg gacgcggcgg gcctggacga aatggaccgc cggctgctct ccgcgatcgt
     4801 gttgcactat gatggcggcc cggtcggcgt cgagacgctg gctgcagcgc tggccgaggc
     4861 gcgagacacg gtcgaggacg tttacgagcc gtacttgctg caacagggct ttctcgggcg
     4921 caccccgcgt gggcgcgtgg cgacgcgcaa agcctacgaa cacttgggtg tgccgttcca
     4981 cggggaaacg cggcaaggga gcctgttttg agcggcaacg cggatcgtta cccggtgcgc
     5041 cccggggcag aggcttatcg tgcggtcgcg cgccctggcg ctccagccct gcgcgcccgt
     5101 acgggcgtgg cgctgatcca cgggtttacc ggctctcccg tttcgatgcg accgctcgcg
     5161 gagctgttgc acgagctcgg tttttcggtc gacgtgcccc gtttgccagg acatggcacc
     5221 cacccgcggg acatggctcg aacgcgctac gcggactgga gacgcgaggt gctcgacacc
     5281 atcgatcgcc tgtcgcgcgt tacggagcgg acggtactag tcggcctgtc catgggcgga
     5341 agtttggtgc tcgacgtcgc atccagtaac gagcgcaccc tggccggtgt ggtggtgatc
     5401 aacccgcagc tcctcgaccg cggcggagcg gcggtgaaga tggcgccgtt ggtcgagcgc
     5461 gtgctgccct tcgtgccggg cgcgctcggc gggctgcgca aagacgacat cgcgaaaccg
     5521 ggagtgagcg agcaagctta cgattggttt cccaccgccg ccggcaactc tttcatggcc
     5581 gagctgcccc gaattcgcga gcagctgaat catctgagtt gcccgctgtt ggtcgcgcac
     5641 tccatgcggg accacagcgt accgcccgcg aactcggagg cgctcttgaa ggctctaccc
     5701 catgctcgag agctgcgcct cgagcgctcc tatcacgtgg cgactctgga ttacgacctg
     5761 gagctcctcg cggaaaagat ctgcgaattc tgcgagaatc ctgccccggc caagcacgca
     5821 tgagataccg cgccgtcgct gtcgccagcg ccgagagcaa cgcggtcggt gccgtggagc
     5881 tggagtgcac cccgcacggg ttgttcattg cgtatctggg cgtcggagcg ttcaccgaag
     5941 gttacgcgcc cggcgcgttg gcgtccggca ccgggctaac ggtgccatgg actcaagtca
     6001 gcgaggcgag gctggaaggc gagcgcctgc tacttgcgat tgatcacaag ctcacaccgc
     6061 tcaatcgctt actcttgacg gaattcgaga cgggccggcg cattcccaaa gaggaactgg
     6121 ccaaacgtcg gctcctggtg cgcttctcgt cactcgcggc ggcgctggtg gccgcattga
     6181 ttctgggcgc cttcgtgctg cgctcctcac cggagacgtc ggcgagcgcg gccgtcgccc
     6241 tggcgctgct ggcagcgttg gtgctcatga ccatgggctt cctcgtcgat cggagcatgg
     6301 agagcagcgc cgaagaccgc gctgcattcg agaactttgc ccgtgaaatc agccaatatc
     6361 tgcctacgct cgtgcagctg ccgcacgcgc cgcgcgctcg caagccgttg ccggacgtct
     6421 ccgagcttca gggtctatta ccgcgcacca cgctggcgat cgtaatcaca ctgacctcgg
     6481 gtttgctctc ggtgctgcta gtggcgcgct gggtcaccaa caatcaggcc gaggtgcgta
     6541 gcgtgacgtc acgcatgctc gaacctgcag cacctcgaga agcggcggca gccgcccgtc
     6601 cgcaagcagc gcccaaggca cccaagccca gctcaccacc gcaggtcgcg gccgcacgtg
     6661 atgttgccgc gacgcccgcg agcagcgggc ccgggcaagg cttcggagac gagtgccgct
     6721 gcgtgcggcc cgactctgcg ctgtggagcc gcccgctccc acgtttgagc gtactgacga
     6781 tttcgaagcg ccttcggctc ggcaagaacc ccgacgagaa caagcgcaag aagtacctcg
     6841 agctagatct cgcggtggtc aacaactcga acaaggatat cgaggagatc gccttgatgg
     6901 tcctgttcta cgatcgcgat ccgccccctt cgaaccggcg caatcaggtc tcgaaccggc
     6961 cgctattctt cgaaggcccg ctgcaacctg gccaagccat caagtggagc gtggaggccg
     7021 agggcacgga gttcgaagtc caaaacccca ttgcgggcag tctcacgccc gaaggggacg
     7081 atatcgcgcc cgccaaccgg ctcgcggagc tgctcagcgc acgcaatcgc cccgtccgct
     7141 tgcacggcgc gatgctgctg accttcctat ccgatccgcg cgcccgcgaa ggggcgctcg
     7201 ggttgcgcga agcgctgcgc gaggatgaag ccccctatct gtcccgtttg attcaagcgc
     7261 tcggcgacgt acgagtttgc cagctgcgcg taagctcggg ccccggcccc gcgcgcacgg
     7321 tcaacgcctg tgtgcacaac accagcaagg agccgaaaaa ggagctcggc atgaagctgc
     7381 gtgggctcga gaacgccccc gatctcggca atccgtcggc ggatccaccg acggtgcagt
     7441 tcgagacgac ggtgcacctg cccggcgagc tcggcgcggg cgccgcacgg acggtacgtt
     7501 ttccggtcga cctcgggagc gcagccccga ccatttacga agcgtacgtc gatcgcttcg
     7561 atctcctggc gtccccttga ttggatgaac tcatggcagc actcatgaca gcagagacgc
     7621 tcgcgattcg ccccgcccgc cccggcgacc aacacgcaat cttcgacctg attcgggagc
     7681 tcgcgcgctt cgagcgcctc gaacacgtag taagcggcag cccggacgca ttggctcacg
     7741 atttgttcgg ggagcgacca agcgccgagg cgctgctggc cgagagcgac ggcgtaccga
     7801 ttgggtttgc cctcttcttc agcacctact cgacattcct gacccgacct ggcctttacc
     7861 tggaagacct cttcgtgctg gagccatatc gcggccaggg tgtaggccgc gccttgttac
     7921 gcgctgtgca tgagcgggcg cgtgaacgcg gcgcgggccg gctggagtgg tcggtgctgg
     7981 attggaacga acgtgcgatc gcgttctatc gcagcttcgg cgcgaccgtt ctgcccgact
     8041 ggcggatctg ccgtatcgag ctctcgccaa cgacatagaa cgagcgttca ctcgaacaga
     8101 cagagtgact ccacgtagtc cttggacccg tcacgcagcc aaccatccag ttgcgcgacg
     8161 tccttgagct gctgtccacg cgggcgcggc acacagaaga actcggtctt cacgtcgggc
     8221 aaggccgtca tgtcgcccca caacttctcg aactgggtca ccggaaatac gtcttcctgc
     8281 gcatttcccc agcgccgctt cacctctttc agcgtcacgt acgtgggcaa gcgctgagcg
     8341 agtgagcgca ccccgcgcag caccgccttc tcgtcctcga gggcggcgcg gttcaaacca
     8401 aacaactcga gcaaacggtc gagtgagatg aaggtggtca gcgagttgta ataagtcagc
     8461 ttggcttcgt cttcctcgcg tgggaaagcg agcccttcca ccagccgcgt cctccccccg
     8521 acccgggcca gaccaccgcc acgatcctcg agacgccgct cgaccacttc gaaactgaag
     8581 gcggccccgc tctcgacgtg gcggcccagc cagcggggat ccagatctgc accgagcgta
     8641 tcgatgttgt gaagcatcgc gtacttgagc tcgggtcgcg cacgcagcag ccgacgcaga
     8701 gtgccattga gcaacaaatt cgcgacctcg taccaatggc ccaccgggtg caaacactgg
     8761 gaaggcacgt tgtctcgata gtcgctggct tcacccgtgc ggatggccca ttcgatcagc
     8821 gcggcgcgtc ccgcgtcgcg gaccttctgt ttgcgttcct cgagcttttg ctcgcgcagc
     8881 tcggcccacg caaaacgcag gtcatgcgcg gtcggcacca accgcaaacc aatggatcgc
     8941 ccctccgata ggtgcaccga ttcgggcaaa ccgtaagcgt tctcacgctc gatcaagccc
     9001 gcaatcgggg cgtgggtcag gtaactcgtg gtgagcacgt gcggcggtat cacgccgaac
     9061 tcccgaccac gtttgcgcgt cttggccaga tgcacttcga tgaaagtgcg gaatcggttg
     9121 cccagcttgg caaacgggta gatggctttg accgtgcccg cgccgtgcgt ccagcgcgtg
     9181 cccgctccgg cggccagcgt aatgacgcaa acttccccgc gtgcgagcgc cgcgctgccc
     9241 gcgtccagct cgcgcggccc gagcgcggcc gcgtgcacca cgtctttcgg gtgcacgtct
     9301 tccacgctcg tgtcggccgg cagtcggttc ttggcgagcc cgatgcgctc ggcacgcaag
     9361 tcggcgcgga tgcgctcgtg ctgctcggcg tcgaagccgt agcgatccaa cagagcctgc
     9421 agagattcct cggcctcacg cgactgggtt gcaggaagga gccgtccgag caacgcctgt
     9481 ccaacccgct ccgcgcgttc cccacgcagg tgctggcgcg agaaggcgtc gatttcggca
     9541 cgacgctgcg gagagaaaga gcgcgagccg tcccgcagcc agcgcggcaa catgcaaagg
     9601 tagtactcgg ccgagaaggt ggccgcctct ccgctcagca gctccgctga tgatccctga
     9661 ccattgaccg agaaccggta gacgactgga tccatcgcga acggcaggct agcggccagc
     9721 tcccgcttgc ggcggcgcat aatctccagc aatcgaactt cggcttccgc cttgacgctc
     9781 ggatcgaaga tgaagcccat gcctccgccg gacatgccgc ccagcatcca gaagccccag
     9841 aatttctcgc cgaaggccgc gcgcgcgtcc gcaatcagct gctcggtata gaagttcgag
     9901 acccagggaa tgatggtggc gagcggcccc tcgaacaggc gcgtggtgag ccgaccgagc
     9961 tccctcatgt cgccctgcgc gagcgcggcg acgacttgtt cgaaggcgct caagagctct
    10021 tgccgagcat gccactcgcg ctcggtaccg agcaggaact tttcggtcac catctcgagg
    10081 ataggcccca cattggcggc catacctccg tgcacgacta cgagtgactc ttccaaacgc
    10141 cgctgagcgg caggtgagac ccgatcttcc ccgagcaacg tatgtcgggg cagcaagcgg
    10201 ccccggctcg agttccactc cacatcccct tccgccgcgg gcacgccttc gatcaacttg
    10261 attccaggcc aaacaccgcc gctgtcctgc caacctccgc ccgatccgcc gagccactcg
    10321 ccgaggatcg cgcgcgccgc caccatcctt cgttcggcct cggtgaggcc gccagtgagc
    10381 gagctgattt gacccgtggc cctcatgcac acactaatca gaccgccgag taaattggtg
    10441 gagacggcga gccgggatcc ctttgggatg tcgttgacct gactggcgag ctccaagcca
    10501 cgccccggcc cgacgagttg ctcgagcagc aaagaaagag gctgctccga gccctcgagt
    10561 gcgggcggca cgatcccgga cgcgatgacc gctgccttga gtagtcccaa gtagtcactc
    10621 gcgtaattga acacctcgga cgtctcggtc aggtccgccg cggcgtccaa atcgacgctc
    10681 accaggcgca gcaccggctc gtcgatcacg cgcaagtaac agctaatggg cggcgccgtc
    10741 cgcaggtcac gcccgtgcac cgccaaatcg accgaaacat tgaggacgcg tgcgccctcc
    10801 gggtaatcca tccccaaaaa gaagatgtca ctccaaccgc tgtgggtcag atccatacgc
    10861 acgggcgtgc gctcgaccag caccggataa aagccagcgg ggtcgggttg cttgagccgt
    10921 tcgtgaacgc gcaaaggctg atccagcgcg tggccagccc gaaacatcca gcggttgccg
    10981 cgcagtgagc gcacggatct ctggacctgc tcggagagca gctggaaacc gagcccgtgg
    11041 tacgccgccg ccagcgcgct cgacagcggg tcattcaaac cgtgctgatc ggcctcggcc
    11101 agaaacgcat ccgtcgccgc cgcatagtgc cgcgcgagca gcagctcgtg gccttgacgc
    11161 gggatgcgcc ccgctcggcg cagctcggga cgagctggga tccaatatcg atatagcgcg
    11221 tgcgcttgaa agatggcgcg cacacgctga tacaggttgt gttcgggctg gctcgcgaac
    11281 tcgtggagct cgcgcgcggc gcgagtcaac tcggccaagc tcgcgtggtc ggcaaagcgc
    11341 tcgagcactc cgatgcgagc ttgcggttcg ctctgcaaga tcgcggcaat cagaacgtga
    11401 ggctcggtca cggtcagcct cgctcgctcg gggggagggc cacctcttcg aggatcaacc
    11461 gcagcacctc ttcgcggcgc gctccgtaga gcgacaaggc cagctgggcg cgcagcagtc
    11521 cgaatgggct ctccaagtcc actcgctgcc cgtccacctc cagcgcgaga tacttctcgc
    11581 gcgtggcaag cagatcgagc gcttcggtca ggccaagctt gccgtctggc gccaagccgt
    11641 cgcgttgctc gcggagcacc tggaaaaccg tggggctcag cgcatgcgtg ccgaacaacg
    11701 cgaggtacgt gccttgccga aggccggcga tcatgcagcg ctcttctgca accgtaggcg
    11761 tgggcttctc gatcaccgtc tgcacgctat acaggcggga cgcgcctttg acgcgatttc
    11821 cgccgaccac cccgaagtag ggcagctgat tttccgcgtt tacctgcacg ccggagacgg
    11881 cgcacccttc tcgttcggct gcctccacca gttgccgggt acaggaagct ttggcgtggg
    11941 tcacgaacac gtgatcgcaa acctgcacca cgaagggttc cccagcgacc caagactccg
    12001 cgcacagcac cgcgtccccg aagccgcgcg gctccagttg ttcgatggag accagtgcgt
    12061 tgccgaactg accgcgcagc tcgccaaata cccgatcacc gcccgggcgg gtcacgagcg
    12121 ccacctgcga aattcccgcc tccagcagct cctcgatttg gagcgcggcg acgcgcttgg
    12181 tccccccgtc tgccgtagtg acgtgctgca aaggcagatc cgcatgaacc ggacgaccca
    12241 tagggatgat agcttttttg attctcacgg cgaaaccctg gcaccttctg aaagcaagcg
    12301 acctcgaggc ataacattcg ggggcgccct gcgtaaactc cgcgcgatcg ttcgcgccaa
    12361 ctctgcgcgc caactcgcag gcctcgtgtc gatttgcgcg tgcggcaaag cccccaatct
    12421 ccgcttgatc gagcgaagcc gtcctgacgc cgctcggccg gtgcttgcgt ctccagacgg
    12481 tcaactcgag cccgccgacg gagccctcca gcagatccag cgcggccaca ctccccagcg
    12541 ttcatagacg cacccgccgg gcatctcttg gcttccgcca caccgcttcg caagccccgc
    12601 gccccgtcgt cccccaacga gctcactgtc aaagctcact ttcaaaatat attttgtcga
    12661 cactcgagat ttgcgaaacc gggacacccc atttgctttg caaatatcat atatcggtaa
    12721 gcgactaagg cgagcgcgcg cagagttcgc cggccgcccc acggcgcaac gaaccgagtg
    12781 ggaaattgaa tagggcgcat ctggatcgca gcggtggttc gcggctgtta gggacagaga
    12841 gggcttgccg tcagcggcgg caggaaagac gagaagcgaa cgaggcaggg gtcgagagcc
    12901 gcgcggagag acgccaagtt ccctggaaac gtcaaaaatt tcgctgatgc tccaaaaatg
    12961 agacccacat tcccgctcaa atgagaggac ggtgggtgta gggcagcaga ccatacgcgc
    13021 cgcggagtct gcccctggcg acgtccgcca gacgcgcatt gcgtcgccat ttttgcgagg
    13081 tacttttcaa gttcagcatg gcgcagtctc gagatcgtcg acgcattgac tggcgcatca
    13141 tcatggacgg gaagcttccc gcagctcggc tttcgaatcc gacgctgccg gtgaagcagc
    13201 tcgccgcgtt gatcgccgaa gcgatgagcg atccgacgcc cgagacgccc gatcgcatca
    13261 tccggcatac cgtcgacttc tccaaggaaa ccctgcagct cgagcgcagc gccgtatacc
    13321 tgttggacga agagaacttc ttgatgactg gcacctgggg cagcgacgct cagggatcga
    13381 cgatcgacga gcacgcgctg atctatgatt acggcccgat cgatcgcgag gtgttcgcgc
    13441 gggctcaagc tggttttcct tggacggcct acgacgattg cccgcacatc gcgcaggttg
    13501 gtgaagagac acaggtgatc ggtcaaggtt gggtggcgtg tacggcgatc gtcgggccga
    13561 cagggccgct cgggatcttc ttcaatgaca cggccatctc gcacaatccg ctcgacgaat
    13621 cgagacagtg gcgggccgca attctgtgct cgattctcgc gaatgcgctg tggcgattga
    13681 agacggccga cgtgagcacc acggcgcacc gggagacgcc gcagccggag atcgtgaggc
    13741 gggtcgcgcg gctactcact caagacccga cgctgagctg cgacgcgctg gcgcgccaat
    13801 tgaacttgag tccgcggggc ttggctcgtt ccttcaaga
//
LOCUS       DASTKU010000005         5288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1338809, whole genome shotgun sequence.
ACCESSION   DASTKU010000005 DASTKU010000000
VERSION     DASTKU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338809"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            463..1113
                     /locus_tag="VFQ61_00135"
     CDS             463..1113
                     /locus_tag="VFQ61_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952873.1"
                     /translation="MGVLPYPEQEDNSSRGETQSRGGSAGVTGDFDGGAELGGCSPSG
                     GAAVETGGAPGVNTTNSSAGAGGVGRDDCFDEPQRALCEVYRKRPNTCVYPDDVCFCD
                     YFSPLPYDSAWFCYPRGCPQVLPRGRCASGSEDEVNRECVYGPTKCICAAPVEGIGFW
                     HCDHHIECPEAAPTEGEVCESIDDDVENNTCVYGTTRCSCGYAPGYPGKWRCSQMY"
     gene            1826..3193
                     /locus_tag="VFQ61_00140"
     CDS             1826..3193
                     /locus_tag="VFQ61_00140"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9952874.1"
                     /translation="MSGVTTQTAATSELLETNGEQRMTVIRWVYPLPARAPTWLGTSS
                     LTLGRDEGCDVRLDGDRVSRRHASIRRSGSLWLLDDLDSKNGLFINARRSPGAALENG
                     DVIRIGDFVGVVMEVPEGTDLAFGDLGHEICGGYRHRRAVEKIRAVATASIPVMLQGP
                     TGTGKERFGRALHAWSGRAGAFLAVNCAAYSPAIAAAELFGHRKGAFTGAEQASPGHV
                     RAAEGGTLMLDELADLPLHVQAMLLRVIENREVLAIGETQPRAIDVRFVAACQVPLSE
                     ATARGSFRPDLRARLEGAVIELPSLHDCQEIVPELFCELFLRHSGRAPELSAVAAERL
                     CLHPWPLNIRELEMLARHLAATHASGAQISEALLEKLPRSAPSDAPPPRTAEPKPRKA
                     VAVPGRTDSTYPESELRSLMDALTQCDGNVTKAAALLGLSRPRAYRMLEVAQRAGMVA
                     PRGDR"
     gene            3233..4666
                     /locus_tag="VFQ61_00145"
     CDS             3233..4666
                     /locus_tag="VFQ61_00145"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9952875.1"
                     /translation="MQSRDPAEGSPSMPEAGIIQAGTILDGRFRVERLIGQGGMGYVL
                     AAMHLELQQRVALKLLSPNREQRERSASRLSREARASARIRSEHVVRVLDVVHSPGSP
                     PYIVLEYLEGEDLAHRLSRCGRLEIAEAVEIVLQACEAVGEGHRLGIVHRDLKPSNLF
                     LCSRGGQAVSVKVLDFGISKMDEAGASLTQSHALLGSPVYAAPEQLRSSREVDARADI
                     WSLGVVLYECITGARPFDGQTLAQVCTSILHDSPRSPRALNPQLPPALEAVLLRCLRR
                     KPDQRYSSIEELVRALHDFAPRAAERCASYLDALPPAEQARVSHIPIRESVRPEPSDT
                     LTGSAEYDSHDGTASLVRVGTDSRDRARWSLLVAAVVFGALVVAWQLLRSLPPTAAVD
                     LAAHSASEATPTGAPRATDIPKSSQSTPALAGDSLSPAPTPTVSTQPVEVVPTSTANR
                     DSSNRTAGPRPASPKGRPAIAPWVDTR"
     gene            4766..>5288
                     /locus_tag="VFQ61_00150"
     CDS             4766..>5288
                     /locus_tag="VFQ61_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952876.1"
                     /translation="MSSAQLAAEVLFLDGIKLMKEGRCADAVTKFHDSQELDPASGTL
                     LNLAYCQVTLDRIASAWLTYRQALALAQASQKTQHAQIAREQAAKLEPVLPRLVLRSA
                     DAGARELIVELDGAPLTPSVWSLPVPVDPGGHHVRVKLEDGEVWQTELNISRAQRAIV
                     DIPLPVAPTATPTA"
BASE COUNT          966 a         1564 c         1747 g         1011 t
ORIGIN      
        1 cacgcctggc tcacggatga agcaatcaac tccccgtgag cagcgtcatt tcgggcgcgt
       61 tcgaatggcg caggcaacga acaacgatcg cgaactgatt cgctgcggcc gacccatgag
      121 caatgtcaca ttgttccgaa ggggatatat tcaatctgtg gcgtggtcag ctcgcaccgc
      181 ttttactggg gagcggcgcc gtacgcattt gatgtctata tttgctgtgc ggcgcaggcg
      241 agatgagtcc gtggagtcca attttcaagg gaggagacca tgaaagcacg tggatatgtt
      301 tcgagtcttg caatactcgc actgccgtac agtgcgtgcg ctggggatct caaaactgaa
      361 tcagcggcga aaggcggcag cggcttcacg ttcgatgcga gcgtcgcagg ctggcaagcg
      421 cgggcgagaa cgagggctct tgttcacact gagagcggcg gtatgggagt cctgccgtac
      481 cccgagcagg aagacaattc gagtcgtggg gaaacccagt cgagaggtgg aagcgccggc
      541 gtgactggcg acttcgacgg cggagcagaa ctcggtggtt gcagcccttc tgggggcgcg
      601 gcagtggaga ccggaggcgc tcctggcgtg aatacgacga atagctccgc tggcgcgggc
      661 ggcgtcgggc gagacgactg ttttgatgag ccgcaaagag cgctttgcga agtgtaccgg
      721 aagagaccca acacgtgtgt ctaccccgat gacgtctgct tttgcgatta cttcagccct
      781 cttccttacg actctgcctg gttttgttac ccgcgcggct gcccacaagt gctaccgcga
      841 ggccgctgtg cctcggggag cgaggacgaa gtcaaccgtg agtgcgtcta cggcccaacc
      901 aagtgcatct gcgctgcacc agtggaagga attggtttct ggcattgtga ccaccatatc
      961 gagtgtcccg aggcagctcc cacagaaggc gaagtctgcg agagcatcga cgatgatgtc
     1021 gagaacaaca cttgtgtata cggtaccacc cgttgctcct gcggctacgc gcccggctat
     1081 ccgggcaaat ggcgctgcag tcagatgtat tgagccgcgt gacggacagc gcgcggggat
     1141 tgaagggaat gttcatggtg tcgcccggag accaacggcc cccgccgccc gagaacgcga
     1201 ttgacctgtt tgccgatgcg aaccgtgacg tgcacggggt gatagtgccg atgggcccgg
     1261 cgtttcgcgt gggggacgct ctcggctccg ctctttcgac cgctctttcg accagcccct
     1321 tttcgtttgc cgccccaatt gcgttgagaa tgccggagat cgagactgag ttacccgcgg
     1381 ttctttccaa gcgcacatgt ggtccccttc tttgtgtgtt ggtgagcata gcagcgggtg
     1441 acgcgagtac aaatattttt gttgaacacc tagaaggcac tgagtgctcc gccgcttcaa
     1501 gttcgtgtgg gtacgcggtg agattggagt gtgagcgggt gcccgtgggc acgaggccga
     1561 tcttgaatcg gcgatgttgc ggatgagggc acttccggag gctgcgaacc tcggccagga
     1621 aacacatggc cggtagctgg aatatttcct gcgcagccct gcccttgctg tggggcggct
     1681 cttcccgtcg cgcgctatgg gtacgagcga acgcgggaga gtgagcgaaa gcagcgtgct
     1741 tgggctgagc tggcattctg gacgtcgggc ggaaaccggc gcctgctgac ttagctcgct
     1801 cggtctgata gtctggtgac cgaccatgtc cggggtgacg actcaaaccg ctgcaacgag
     1861 tgagctgctc gagacgaatg gcgagcagcg aatgacggtc attcgatggg tgtatccgct
     1921 gccggcccgg gctccgacct ggctcgggac ttcgtctctg accttggggc gtgacgaggg
     1981 ttgtgacgtt cggctcgatg gggacagagt ttctcgtcgc cacgccagta ttcgacgctc
     2041 ggggtcgtta tggttgctcg acgatctgga tagcaagaac ggtctgttca tcaacgcgcg
     2101 acgctcgccg ggtgccgcgc tcgagaacgg cgatgtcatc cgcatcggcg atttcgtcgg
     2161 ggtggtgatg gaggtgcccg aagggacgga tctcgctttc ggcgacttgg gtcacgagat
     2221 ctgcggcggg taccggcatc gacgcgccgt ggagaaaatc cgcgcagtgg cgaccgcatc
     2281 gatccccgta atgctgcagg ggcccacggg aacgggcaag gagcgattcg gtcgggcgct
     2341 gcacgcttgg agcgggaggg ccggcgcgtt cctcgctgtc aactgcgccg catactctcc
     2401 cgcgatcgcg gccgcggagc tgttcggtca ccgcaagggg gcgttcacgg gagctgagca
     2461 ggcaagccct ggacacgttc gcgcggccga gggcggcacg ctcatgctcg atgagctcgc
     2521 agatctgccc ttgcacgtgc aagccatgtt gttgcgggtc atcgagaatc gagaggtgct
     2581 ggcaatcggc gaaacgcagc cacgagcgat tgatgttcgc ttcgtggcgg cctgccaagt
     2641 tccgttgagc gaggcgaccg cgcggggctc gttccgaccc gacttgcgcg cgcggctgga
     2701 aggcgccgtc atcgagttgc cttcgctcca tgattgtcag gagatcgtcc ccgagctgtt
     2761 ttgtgagctc tttttgcgac actcaggcag agcacccgag ctgagcgcgg tggcggccga
     2821 acgcctgtgc ctgcaccctt ggccgttgaa tatccgggag ctcgagatgc tggctcgcca
     2881 cctcgcggcg acgcatgcga gtggagcgca gatttcggaa gcgctgctcg aaaagctacc
     2941 gcgaagcgct ccgagtgacg cgccgccccc gcgaaccgcg gagccgaaac cccgcaaggc
     3001 ggtggccgtg ccgggacgaa ccgactctac ttaccccgag tctgaactgc gctcgctgat
     3061 ggatgcgctg actcagtgcg acggaaatgt gaccaaggcg gccgcgctcc tgggcctcag
     3121 tcgaccgaga gcctaccgca tgctagaagt tgcccagcgt gcgggaatgg tggctccacg
     3181 cggtgatcgc tgaagacaat cgaacgctga agacaatcga tctttaagga caatgcagtc
     3241 acgcgaccca gccgagggtt ccccttcgat gcccgaagct gggattatcc aggccggcac
     3301 catcctggat ggccgcttca gggtggagcg gctaattggc cagggcggga tgggatatgt
     3361 gctggccgcg atgcacttgg agcttcagca acgagtcgcc ctcaagctgc tgtcgcccaa
     3421 tcgggaacag cgggaacgaa gtgcgtcccg cctgtcccgc gaagctcgag cgtccgcgcg
     3481 gattcggagc gaacacgtgg tgcgcgtctt ggatgtcgtg cactccccgg ggtctccgcc
     3541 gtatatcgtg ctcgaatatc tggagggcga ggacttagct catcgcttga gtcgatgcgg
     3601 ccgcctggaa atcgcagagg cggtcgaaat cgtgctgcag gcgtgcgaag ccgtcggcga
     3661 ggggcatcga ttggggatcg tgcaccgcga cttgaagccc agtaatctgt tcctgtgctc
     3721 acgcggcggg caggctgtga gcgtgaaggt gctggatttc gggatctcga agatggatga
     3781 agcgggggct tcactcacgc aatctcacgc gttgctgggt tcacccgttt acgcggcgcc
     3841 ggagcaactc cgctcgtcgc gcgaggtcga tgcacgagca gacatctggt ctttgggggt
     3901 cgtattgtat gaatgcataa cgggggctcg tccgttcgac gggcaaacgc tcgcgcaagt
     3961 ctgtaccagc attctgcacg acagcccgcg ttcacctcgc gcgttgaatc cgcagctgcc
     4021 accggcgctc gaagcggtat tgttgcgttg ccttcggcga aaacccgatc agcgttactc
     4081 ttcgattgaa gagctcgtac gcgcgctgca tgactttgcg ccgcgcgcgg ccgagcgatg
     4141 tgcgagctac ttggacgcgc ttccgcccgc cgaacaggcc agagtttcgc acatcccgat
     4201 tcgtgaatcg gtacgccccg agccctcgga cacattgacg gggtccgccg agtacgacag
     4261 ccacgacggg accgcatcgt tggtgcgagt gggcactgat tctcgcgatc gagcgaggtg
     4321 gagtttgctg gtcgctgcgg tggtgtttgg ggcgctggtg gtcgcgtggc agctcctccg
     4381 ttccttgcca cccaccgcgg ccgtggattt ggccgcgcat tccgcgagtg aggcgacacc
     4441 caccggcgct ccccgtgcaa cggatatccc gaaatcaagc caaagcacgc cagcccttgc
     4501 gggtgactcc ctctcgccgg caccaacacc cacggtatcc actcaaccgg tcgaagtggt
     4561 ccccacaagt acggccaatc gagactcatc caatagaaca gccggcccac gccccgcatc
     4621 gccgaagggg cgccccgcga ttgcaccttg ggttgacacg cgttagtcgg atttcgtgaa
     4681 gtgggtggct ctccacagct cgattggcgt actggcgctc agtttgcgag cgaccccagc
     4741 gttcgctgag gaggcaggtg cgaaggtgtc ttcggcacag ctcgcggcag aggtgctctt
     4801 cctggacggc atcaagttga tgaaggaagg ccgctgcgct gacgcagtca ccaagtttca
     4861 cgacagccag gaactcgacc ccgcaagcgg gacgctgctc aatcttgcct actgccaagt
     4921 gactttggac cggatcgcta gcgcctggct gacataccgg caggcgctag ccctcgccca
     4981 agccagccag aagactcagc acgcacagat cgcacgagag caagccgcca agctggagcc
     5041 cgtgttgccc cgcctggtgc tgcgttcggc tgacgcgggg gcgcgcgagc tcatcgtcga
     5101 gctcgacgga gcacccctca ctccctcggt gtggtcactg cccgtgccag tggatccggg
     5161 aggccaccac gttcgcgtaa agctcgagga tggcgaagtt tggcaaaccg aactcaatat
     5221 ctcgcgagcc cagcgcgcga tcgttgatat tccgctgcct gtagcgccga ctgcgacgcc
     5281 aacagcgg
//
LOCUS       DASTKU010000006        10350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1561714, whole genome shotgun sequence.
ACCESSION   DASTKU010000006 DASTKU010000000
VERSION     DASTKU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10350
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561714"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(682..2094)
                     /locus_tag="VFQ61_00155"
     CDS             complement(682..2094)
                     /locus_tag="VFQ61_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9952877.1"
                     /translation="MSVRRFPRITIPGMLSALSLIVSAACSPAAVDYAELRSAAQARL
                     PARSLAPESNDVKALLRAPITPDSAARIAVLNNRGLRASLEDLSIANAELSRARRLPN
                     PTLEGALRYHRHGGTDLEVGALIDLSSFLLLSVRSRAAEAQVAATRLASIGAILDLTF
                     DAKRAYYEYQAALDALELRKTVSRALAASAEAAQRLHDAGNITDLELASESSLYEEAR
                     LTEQRASAHLAAAREHLNTVLGVFGNADRWSTSGRMAKPPAEELAVNALESSAVKRSL
                     ELRISEQRYAAAAQRENFARARGLLPELKAGVSAEREESWGVGPAVEIEVPLLYQGQA
                     DVAVARAQKRKQIETYADLAVRVRSNARATATRLGAARDAVLYYESTLLPLKAKIVEE
                     TQLKYNAMLIGVFQLLQAKRDQVETAARYVEQLKEYWLLRTDAEQLLSGRLTPGNSAF
                     ASPSNTSEMPSTAGAMRDAH"
     gene            complement(2091..2411)
                     /locus_tag="VFQ61_00160"
     CDS             complement(2091..2411)
                     /locus_tag="VFQ61_00160"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HET9952878.1"
                     /translation="MMRFTAAPYALLLSIACTSTQLPVPADHPARPDAAVAPLPVIPS
                     LSAASIHSVGGEHAAESGPGHADHAPAANVKYTCPMHPEVVRDAPGNCPHCGMKLVPK
                     QASQ"
     gene            complement(2475..3800)
                     /locus_tag="VFQ61_00165"
     CDS             complement(2475..3800)
                     /locus_tag="VFQ61_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HET9952879.1"
                     /translation="MVSRREALKVAPAAVAGAALLARSAEAAPGFAASYSGGPPDAPP
                     SSPGMPGRDYTPTLTPNGATLPFKIVEGAKVFHLVTSEMEHEFAPGLKARCWGYNGRV
                     HGPTLEAVEGDRVRVYVTNRLPAPTTVHWHGVFLPNGMDGVGGLTQRVIRPGETFKYE
                     WTFRQHGTYMYHSHHDEMTQMALGMLGMIVVHPRRPAPEYHVDRDFVLLLSEWRIDPG
                     TSRPNPNEMTDFNLLTINAKAFPGTAPLVCRKGDRVRIRIGNLSAMDHHPIHLHGHYF
                     KVTATDGGRLPASAQWPEASILVPTGSTRDIEFIADAPGDWPLHCHMTHHVMNQMGHD
                     IPNMIGVKPGTLDKKIRPLLPGYMSMGETGMGEMGDMGMAVPKNSIPMVGAPGPFDYI
                     TMGGMFTILKVREGLKDRDHDPGYYKHPPGTVASAASAEDLRRDGIELP"
     gene            complement(3793..4023)
                     /locus_tag="VFQ61_00170"
     CDS             complement(3793..4023)
                     /locus_tag="VFQ61_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HET9952880.1"
                     /translation="MSQTQNTTLDVEGMSCNSCVKHVNSALRGLDGVQDVQVDLKGGR
                     VSVAHDPKLPTQQLIAALEEAGYPACEKPGHG"
     gene            4392..6827
                     /locus_tag="VFQ61_00175"
     CDS             4392..6827
                     /locus_tag="VFQ61_00175"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET9952881.1"
                     /translation="MNPTTPEPEVCDLTVLGMTCAACVRRVERALGAVPGVVEAHVNL
                     VTNRATVKYSPEAFPSSQAREQQLAAAIERAGYEVPRDDAEVGRTESATTERGKETVE
                     SPQAAPSADTLSVNPISSDKEAQIASQSATSAGESANARRARRLAEAETKELRRLRFD
                     FGVALALSVPLLVLGMSHGAIAFAETTPARWLQLILGSLVVAIPGRRFFGLAWAALRH
                     RSADMNTLVALGVGSAYLYSAAGVLAPRIFAHAEHGALPHLYFEAAGAIVTFVLLGKM
                     LESRARKRLSDAVRGLLAMVPNTAHRLDANGAERECRVDELRVGDRVLVRPGERIPID
                     AKILSGLSAVDESMLTGESMPVDKRPSESVYGGTFNRTGALTVEVTRVGGDTALARIV
                     EAVEQAQGSRAPIARLADTVSAYFVPVVLGIALVTAVAWYWVDPSTTGFAIAVERFVA
                     VLVIACPCALGLATPAAVAVGTGRGAQLGVLVKGGAVLEAASRVNTVLFDKTGTLTEG
                     QPKLIELVNRSSFSDDELLGLLALAEHGSEHPVARALVEAARARGLTVEAETDLLAAP
                     GMGVQARVKGHTIRVGNAAWLARGQIDPTPLEPEAERASARGLTPSFIAVDGELAGIF
                     AVADTPTEFAARVVNELKAQNIEVAMLTGDRASTAEHIARQLGISRVIAEVRPEGKAD
                     AVKAERERGRVVAMVGDGINDAPALASAHVGIAVASATDVAVAAADIALLQGGIQNLP
                     TALGLARATLRTIRQNLFWAFIYNVIGIPIAAGALFPWLGWQLSPVVASAAMSLSSVS
                     VLTNSLRLRRFRA"
     gene            6824..7381
                     /locus_tag="VFQ61_00180"
     CDS             6824..7381
                     /locus_tag="VFQ61_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9952882.1"
                     /translation="MTPRHRELEQLLVDHRTAFLNFITRRLDDTALAEDMLQEAFMRG
                     LSHLDELKTDEALLGWFYRILRHQIIDYYRSRSVGERRLEALAAELAHDPVFTQQEPY
                     ATEPSPCRCVVGIVDHLKPEYASALKRVELDGLPVKDYAQEAGISSNNAGVRIHRARE
                     ALRKEVVRCCGSCADNGCVDCTCQS"
     gene            7702..8658
                     /locus_tag="VFQ61_00185"
     CDS             7702..8658
                     /locus_tag="VFQ61_00185"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9952883.1"
                     /translation="MHTAGRRGATRMVSLDLMRTPAPSPDDPTVYPVEEPVGEDILQC
                     WMLEVLRPLIERWVAERRTPCLVGSDQFIYFEKGQVSKRIAPDIYLLPGVPPNTRIPS
                     WKIWESGIRPSFALEICSLDWHKDYVTAPRLHDEIGTEELIVVDPDYLERREGYRFQR
                     YERTSRGLELQERTQADRVRSGILECFLRLVGTEDEPRQHLRLALDPDGDILFPTQEE
                     AERQRAEAERQRADVERQRADSERQRADSERQRAEAERQRAEAERQRAASESQRAEDE
                     RKRAAAAERRAHEDRQQAETQKQLAEAALARVAELEALLKSR"
     gene            9000..>10350
                     /locus_tag="VFQ61_00190"
     CDS             9000..>10350
                     /locus_tag="VFQ61_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952884.1"
                     /translation="MRRDLSLAGLVCFAAMPLAGCSSEAPASSTSFPQESASEIAEVT
                     GTPDRLAACNQDPRVRAGLVSPEVCAGADVFFRETFAGNGRTCGSCHPVANNTTLDEP
                     FIQALHQTNPADPLFVFERDANLSQLETADLLNAGAVLENLDGFEDPAHKFVSRTVNH
                     VLSLATSTRADPADGTDRSVKERTGWSGDGAPDDGTLRSFLVGAIRQHFTKTLTRREG
                     TDFRLPTQQELDLTRAFQLSLGRTNELDLTQVRLSDADAEQGRQAFLDPNRGRCNVCH
                     SNAGANFLDTGLNRNFDTGVRTVVGNRDLGLTTGNWNNNYFVDGGFGATTPFDADGSG
                     TPNSLGNGTFNPPPLIEAADTGPFFHNNARGPEIEDAVAFYASAPFKNSPAAAELDKR
                     FGASLELAAFDSANIARLLRVLNAAFNLDIALQRLNAANTLASSSGDQGADVQQRLIE
                     "
BASE COUNT         1871 a         3182 c         3364 g         1933 t
ORIGIN      
        1 ctgctagcta ttgcgcgcgc ggcagcgggg ccgttcgacg caccctgcga ccgaagccgt
       61 agacaccctg cgcgcgatcg gacaacatcg tccaatcagc agcaaagact gcgttgcgga
      121 taccccatga ggcaacaact catccctgcg aatctgacga catcgccaaa tcaagttgtg
      181 cgctccgccc tgcgctgact ggagcgcatc tcataacctc acagcgtgca agtgatcgac
      241 tttgatccat ttttcgcgag ggtctttgca ttggactgga aacctgtttg taagttcgtt
      301 gatggacctg acggacggtc agtgagaagt ggttcggaag ttcattcctg gcccggaaaa
      361 gatgcgtacg agcgaagttg gccggccgtg gcgagatcca cgagatgttc tgaatggggt
      421 gcttcgggtc ttgcgaacgg gcgcaccatg ggcggatccg ccgtctcgat accctccgta
      481 tcagacctgc catcgacgtt ttcagaagtg ggaccgagag gggatcttgg acgagatact
      541 gcaggcatcg gtgcagcacc ggctcgaagt ccgtgccgcg aagcgcggca cagaccgctt
      601 ggcgccagcg cgcaactcca gcgtgctgcc tgacttcgcc gacgtgatgt tccctgtggc
      661 gcttctagcc gtccgtgtag ttcaatgcgc gtcgcgcatg gcacccgccg tcgagggcat
      721 ctcgctggta ttgcttggac ttgcgaatgc ggagttgccg ggggtcaagc ggccagacag
      781 gagttgttcg gcgtcggtgc ggaggagcca atattctttg agttgctcga cgtagcgcgc
      841 agcagtttcg acttgatcgc gcttggcttg taggagctgg aatacgccga tcaacatcgc
      901 gttgtacttg agctgcgtct cctccacgat tttggctttc agtggtaaca acgtggattc
      961 gtagtagagc acggcgtcgc gggcggcgcc caggcgggtg gcggtggcgc gggcgttgct
     1021 tcggacgcgt acggcaagat cggcgtaggt ctcgatttgt ttacgcttct gggcgcgcgc
     1081 cacggccacg tcggcttgac cttgatagag gagtggaact tcgatttcta cggcggggcc
     1141 gacgccccag ctctcctcgc gctcggccga gacgccggct ttcaactcgg ggagtaggcc
     1201 ccgggcacgg gcgaagttct cgcgttgggc ggcggcggca tagcgttgct cgctgatgcg
     1261 tagttccaag ctccgcttca ccgcgctgct ctcgagagca tttacggcga gttcttcagc
     1321 agggggcttg gccattcgac cggacgtgga ccaacggtct gcgttgccga acacgccaag
     1381 tacggtattc agatgttcgc gtgcggcggc gaggtgggcg ctggcgcgtt gctcggtgag
     1441 gcgcgcttct tcgtacaagc tggactcgct ggcgagttcg agatcggtga tgttgcccgc
     1501 gtcgtggagg cgctgcgcgg cttccgcgga ggcagctaag gcacgagaca ccgtcttgcg
     1561 gagctcgaga gcgtcgagtg cggcctgata ttcgtagtag gcccgcttgg cgtcgaacgt
     1621 gagatcgagg atcgcgccga tggaggccaa tctcgtggcc gcgacctggg cttccgcggc
     1681 gcggctgcgc acgctgagta acaagaagct gctcaagtcg atcaaggcgc ccacctcgag
     1741 gtcggtgccg ccgtgccgat gataacgtag agctccttcg agcgtgggat tcgggagccg
     1801 gcgcgcccga gataactcag cgttggcgat cgagagatct tccaggctcg cccgcaggcc
     1861 acgattgttc aaaacagcaa tccgagccgc gctgtcgggc gtgattggag cccgcaagag
     1921 cgctttcacg tcgttgcttt caggcgcgag agatcgcgcg ggcagccgag cctgcgccgc
     1981 ggagcgcagc tcggcataat ccaccgctgc cggggagcag gccgcactga caatgagact
     2041 cagcgcggac agcatgccgg ggatcgtgat gcggggaaag cggcgaacgc tcattggctg
     2101 gcctgcttgg gaaccagctt catcccgcag tgcgggcagt tgccaggcgc gtcgcgcacc
     2161 acttccgggt gcatggggca cgtgtacttc acgtttgcgg ccggcgcatg gtcggcgtga
     2221 ccggggcccg attcggcggc gtgctcgcca ccgactgaat ggatgctcgc ggcgctgaga
     2281 ctcgggatca cggggagcgg agcgacggct gcgtccgggc gagccgggtg atcggcgggg
     2341 accgggagct gcgtcgaagt gcaggcgata ctcagaagca gggcgtaagg ggcggcggtg
     2401 aagcgcatca tgtggattcc aagttcggtt caagagtcga ggactcgagt tcgttgcggc
     2461 gtcactggca cgggtcaggg cagctcgatg ccgtcgcggc gcagatcttc ggcggaggcc
     2521 gcgcttgcga cggtgcccgg tggatgtttg taatagccgg ggtcgtgatc gcggtccttc
     2581 aggccctcgc ggaccttcaa gatcgtgaac atcccgccca tggtgatgta atcgaaaggc
     2641 ccgggcgcac caaccatggg aatgctgttc ttgggcaccg ccatgcccat gtcccccatc
     2701 tcgcccatgc cggtctcccc catcgacata tagccgggta acagcgggcg aatcttcttg
     2761 tcgagcgtgc cgggtttcac gccgatcatg ttggggatgt catggcccat ttgattcatc
     2821 acatggtgag tcatgtgaca atgcaggggc caatcaccgg gggcgtctgc gatgaactcg
     2881 atatctcgag tactgccggt gggtaccaaa atcgaggctt ccggccattg cgcggaagcg
     2941 ggcagacggc caccgtccgt cgcggtgacc ttgaagtaat ggccgtgcag atggatcgga
     3001 tggtgatcca tggcgctcag gttgccgatg cggatccgaa ctcgatctcc cttacggcac
     3061 accagcggcg cggtgcccgg gaaggccttg gcgttgatgg tgagcaagtt gaaatcggtc
     3121 atctcgttcg ggttcggtcg cgaggtcccc ggatcgatgc gccactcact cagcagcagt
     3181 acgaaatctc ggtccacgtg gtactccgga gctggccgac gcgggtgcac gacgatcata
     3241 cccagcatgc cgagcgccat ctgcgtcatc tcgtcatgat gggagtggta catatacgtg
     3301 ccgtgttggc ggaaggtcca ctcgtatttg aaggtctctc ccgggcgaat cacgcgttgg
     3361 gtgaggcctc ccactccatc catgccattc ggcaagaaca cgccgtgcca atgcacggtg
     3421 gtcggagcag gcaggcgatt cgtgacgtac acacgcacgc ggtcgccttc cacggcttcc
     3481 agggtggggc cgtgcacgcg cccgttgtaa ccccaacagc gcgctttgag ccccggcgcg
     3541 aactcatgct ccatctcgga ggtcaccaag tgaaagacct tggccccctc cacaatcttg
     3601 aagggtagag tcgcgccgtt cggggtgagg gttggcgtat agtcgcgccc cggcatacct
     3661 ggtgaactcg gcggtgcgtc gggcgggccg ccactgtaag aagctgcaaa accgggggcg
     3721 gcttcggccg agcgtgcgag caaggctgcc ccggcgacgg ccgccggagc taccttcagg
     3781 gcctcgcgtc gactaaccat gaccaggctt ctcacaagcg gggtagcctg cctcctcgag
     3841 cgcggcgatg agttgttggg tgggtagctt cgggtcgtgc gcgaccgaca cacggccgcc
     3901 cttcaaatcc acctggacgt cttgcacacc gtccaaaccg cgcagggcag aatttacgtg
     3961 ttttacgcaa gaattgcagc tcatgccttc gacatccaag gtggtgttct gagtttggga
     4021 catggtcctt ttcctatcgt ctaatcctag tggagttggg gttggaacga cgactcacag
     4081 cgcgtcgctc acctcgaagg acggggctcg ggcctgagca ttacaggact cggaccaaaa
     4141 tcagtcgccc tcgcgcgctg taatgatcct gaccggtctg cgtcttcagg ggtgaccttc
     4201 ccgcggattg ggcgccagtg agcccgccgg ccagcctcga tgccagttcg aatcgtcggg
     4261 ttggcggcga aacggccgat gtcttgcaat ctacgagccc cggcccggaa aagtcatgag
     4321 gagccaggca aatgacgcag caaactcaag taccctcgag caccgggtcc ggaaatatgt
     4381 ccgccgttga agtgaatccg acaactccag aacctgaagt ctgcgacttg accgtgctcg
     4441 ggatgacctg cgcggcttgc gtgcggcgtg tggagcgcgc gttgggtgcg gtgccgggtg
     4501 tggtcgaggc gcacgtcaac ttggtcacga accgagcgac cgtaaagtac tctcccgaag
     4561 cgttcccgtc gagccaggcc cgcgaacagc agttggcggc cgcgatcgaa cgtgcgggtt
     4621 acgaggtgcc tcgggatgac gccgaggtgg gaagaactga gtccgcgacg accgagcgtg
     4681 gcaaagaaac ggtcgagtcg ccgcaagccg ctccgtcggc ggacacactc tcggtcaatc
     4741 ccatctccag cgacaaagaa gcccagattg cgagtcaatc agcgacttcg gcgggtgaga
     4801 gcgcgaacgc gcgtcgggcc cgacgtttgg cagaggcgga gaccaaagag ctacggcgac
     4861 tacgcttcga cttcggggtg gcgctcgcgc tgagtgtgcc gctcctcgtg ctcggcatgt
     4921 ctcacggcgc cattgccttc gccgagacga cccccgcgcg ttggttgcaa ctcatcttgg
     4981 gcagcttggt cgtcgcgatt cccgggcggc gcttctttgg gctggcgtgg gcggcgctgc
     5041 gtcaccgctc cgcagacatg aatacgctgg tggctctggg cgtcggctcg gcctatctct
     5101 attctgctgc aggcgtgctg gcgccgcgga tcttcgcgca cgctgaacat ggtgccttgc
     5161 cgcacctgta cttcgaggca gcgggcgcca tcgtcacgtt cgtcctgctc gggaagatgc
     5221 tcgagtcacg tgcccgaaag cggctttcgg atgcggtgcg cggcctgctc gcgatggtgc
     5281 cgaataccgc ccatcgattg gacgcgaacg gcgccgagcg cgagtgccgc gtagatgaac
     5341 tcagagtcgg ggaccgcgtc ttggtgcggc cgggcgaacg catcccgatc gacgccaaga
     5401 tcctgtctgg cctatcggcg gtcgatgagt cgatgctaac gggcgaaagt atgcccgtcg
     5461 acaagcgacc gagcgaatcg gtctatggtg gtaccttcaa ccgcacgggc gcgctcaccg
     5521 tggaagtaac gcgcgtgggg ggcgacacgg ccctggcgcg catcgtggaa gcggtggagc
     5581 aggcgcaggg ctcgcgcgcg cccatcgcgc ggctcgccga cacggtcagc gcctacttcg
     5641 tacccgtggt gttgggcatt gcgctcgtca ccgccgtcgc atggtactgg gtggatccgt
     5701 ctaccactgg attcgcaatt gcggtagagc gcttcgtcgc ggtgttggtg atcgcgtgcc
     5761 cgtgcgcttt ggggctcgca acgccggccg cggttgcggt gggcaccggc cgcggtgcgc
     5821 agctcggcgt gctcgtcaag ggcggcgcgg tgctggaagc ggccagtaga gtgaacacgg
     5881 ttctattcga caagacgggt accttgaccg aagggcagcc caagctgatc gagctggtca
     5941 atcgctcctc gttttcggac gacgaactgc tcggactact cgccctcgcg gaacacggaa
     6001 gcgaacatcc ggttgctcgg gccctcgtcg aagcggcccg tgcacgtggg ctcacggtcg
     6061 aagccgaaac ggatcttctg gcggcgccgg gcatgggagt gcaggcgcgc gtcaagggtc
     6121 acactatccg cgtcggcaac gccgcatggt tggcacgcgg ccagatcgat cccaccccgc
     6181 tcgagccaga agcggagcgc gcttcggcgc gaggcctcac gccttcgttc atcgccgtcg
     6241 acggtgagct cgcgggcatc ttcgccgttg cggacacacc gactgagttc gcggcgcggg
     6301 tggtcaacga gctcaaagcc caaaacatcg aagttgcgat gttgacgggg gatcgtgcct
     6361 cgacggccga gcacatcgcg cggcagctgg gcatctcgcg cgtcatcgcc gaagtgcgtc
     6421 cggaaggtaa ggccgacgcg gtgaaggccg agcgcgagcg cggccgcgtg gtggccatgg
     6481 tgggtgacgg catcaacgac gcacccgcgt tggccagcgc gcacgtcggc atcgccgtcg
     6541 cgagcgccac cgacgtggcc gtggccgcgg cggacatcgc cctgctccag ggcggcatcc
     6601 aaaacctccc caccgcgctc ggcttggcgc gggccacgct ccgcaccatt cgccagaacc
     6661 tgttttgggc ctttatctac aacgtgattg gcatcccgat tgcggccggg gcactcttcc
     6721 catggctcgg ctggcagctc tccccggtcg tcgccagtgc ggcgatgtcc ctatccagtg
     6781 tttcggttct gaccaactca cttcggctcc gacggtttcg cgcatgactc ctcggcatcg
     6841 agaactcgaa cagctcttag tcgaccatcg aacggcgttc ttgaatttca ttacgcgccg
     6901 gctcgatgac accgccctgg cggaggacat gctgcaagag gcgttcatgc gcggcctctc
     6961 gcatctggac gagctgaaga ccgacgaagc cctgctcggc tggttctacc gcatcctcag
     7021 acaccagatc atcgactatt atcgaagccg ctcggtaggc gagcgccgtc tagaagcgct
     7081 cgccgccgaa ctcgcccacg accctgtatt cacccagcaa gagccgtacg ccaccgaacc
     7141 ttccccgtgc cgctgcgtgg tagggatcgt cgaccacctc aaacccgaat acgcttctgc
     7201 cctcaagcgc gtcgaactcg acggactccc cgtcaaagac tacgcgcaag aagccggcat
     7261 ctcgagcaac aacgccggcg tccgcatcca ccgtgcgcgc gaagccttgc gcaaagaagt
     7321 cgtccgctgc tgcggcagct gcgccgacaa cggctgcgtc gattgcacct gtcaaagctg
     7381 acgtcttcgc tgcgcacaac tattctggct cacgattcta gcaacatcga ggtgctatat
     7441 cgcacgccat acaagggagt gtcgtgcgga tgtgctgtgt tcgagtctcc cgttcgtcta
     7501 ctgcccaatt gcagacggcg cccttctcga aaactcgctt cgatgagctc ggtatactta
     7561 tgaggaggtg aactcctaag cggcggcact cgggatatcc ccagtgcctc gcaatcgaac
     7621 tgcgggatac acccggattc cgccggcttt ttccgttctt ttgttcaggc gttctcgttg
     7681 tcgcgtgcta gatggcgatg catgcacact gccggtcggc ggggcgccac gcgcatggta
     7741 tcgttggatc tcatgcgcac tccagcccct tctcctgatg acccgacggt gtatcccgtg
     7801 gaggaacccg tgggggaaga catcttgcaa tgctggatgc tcgaagtgct gcgcccactc
     7861 atcgagcgtt gggtcgcgga gcgccgcact ccgtgtctcg tcggctcgga tcagttcatc
     7921 tacttcgaga agggtcaagt ctccaagcgg atcgcgccgg acatttacct cctacccgga
     7981 gttcctccga acactcgaat cccgtcctgg aaaatttggg agagcggcat tcggccgagc
     8041 ttcgccttag agatctgctc attggattgg cataaggact acgtcaccgc accgcgcctc
     8101 cacgatgaaa tcggaaccga ggagctcatc gtggtcgatc ccgactacct cgagcgccga
     8161 gagggctatc gctttcagcg gtacgagcga acctccagag gtctcgaact gcaggaacgc
     8221 actcaggcgg accgcgtgcg ctctggaata ctggaatgct tcctgcgcct ggttggcacc
     8281 gaggatgagc cccgccaaca cctgcgattg gcgctcgatc ccgacggtga cattctgttc
     8341 cccacccaag aggaggctga acgtcagcgc gcagaggccg agcggcaacg cgccgacgtc
     8401 gaaaggcaac gcgccgactc cgagcgtcag cgcgccgact ccgagcgtca gcgtgcagaa
     8461 gccgagcgtc agcgcgcaga agccgagcgt cagcgcgcag cctccgaaag ccagcgggct
     8521 gaagatgagc gaaagcgggc ggcggcggcg gagcgacgcg cccatgagga ccgccagcag
     8581 gccgaaacgc aaaagcaact tgctgaagct gcgttagccc gcgttgcgga gctcgaggcg
     8641 ttgctcaaga gtcgttgaat tcgcggcgta agatgcggca ggtttgacgg cctcaagacg
     8701 agctggcgac tagctcctat tgggataggt tctaagccag tcaacggcgt cagatggcgt
     8761 cactcgtaca gcagaggatc acggtgaggt ggggcccccc acgcgggcgg acgcccgcgt
     8821 gaggtggggc cctggcgcgc gcagcggagc gagcacggca ggacgggctt gggttcaatc
     8881 ggcgggggca acctaacgcg caaactgaag tattcgccga aaaagagacg agggggttca
     8941 cccgatcggg cgcggcttaa cacgggtatc gatacagcaa cctacgtgaa gagggtcgta
     9001 tgcgtcgtga tttgagcttg gctggtctgg tttgtttcgc ggcaatgccg ctcgcagggt
     9061 gttcgagtga agccccggcg tcgtccacga gctttccgca ggagagtgcc tctgagattg
     9121 ccgaagtgac gggcacgccg gaccgtttgg cggcctgcaa tcaggacccg cgcgtgcggg
     9181 ctggcctggt ttctcctgaa gtctgtgccg gcgcggacgt cttctttcgc gagactttcg
     9241 cgggcaacgg acgcacgtgt gggagttgcc atccggtcgc caacaacacg accctagacg
     9301 agcctttcat ccaggcgctg caccagacga accccgccga tccgctgttc gtgttcgagc
     9361 gcgacgcgaa tctaagccaa ctcgagactg ccgatctgct gaatgcgggt gcggtgttgg
     9421 agaatttgga tgggttcgag gatccggcgc ataagttcgt gtcacgcacg gtgaatcatg
     9481 tgctttcact cgccacgagc actcgtgcag atccagccga cgggaccgat cgctcggtta
     9541 aagagcgcac cggctggagt ggtgacggcg cgcccgacga tggcaccctg cggtcctttc
     9601 tggtcggcgc gattcgccag catttcacca agacgttgac ccgccgagaa ggcaccgatt
     9661 tccgactacc gactcaacag gagctcgatc taacgcgggc ttttcagctg agcctcggtc
     9721 gcaccaatga gctggacttg acgcaagtgc ggctgagcga tgccgacgcc gaacagggcc
     9781 gccaagcatt tctcgatccg aaccgcggac gctgcaacgt gtgccactcc aacgcgggcg
     9841 cgaacttcct cgacactggg ctcaaccgaa acttcgacac gggtgtgcgc accgtggtgg
     9901 gcaatcgcga tctaggcctg actacgggca actggaacaa caactacttc gtcgacggtg
     9961 ggtttggcgc aacgactccc ttcgacgccg acggcagcgg cacccccaat tcgctcggta
    10021 acggcacctt caatccgccg ccgctgatcg aggccgcaga cacgggaccg ttcttccaca
    10081 acaacgcacg tggtccagaa atcgaggacg ccgtggcttt ctacgccagt gcgccattca
    10141 aaaactcccc cgccgcggcc gagctcgaca aacgcttcgg tgcatcgctc gaattggcag
    10201 cattcgactc tgccaacatt gcgcgcctac tgcgtgtgct caatgccgca ttcaacctcg
    10261 acatcgccct ccaacgcctg aatgccgcca acactttggc ctcgagttct ggcgatcaag
    10321 gggctgatgt acagcagcgg ctcatcgagc
//
LOCUS       DASTKU010000007         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_447076, whole genome shotgun sequence.
ACCESSION   DASTKU010000007 DASTKU010000000
VERSION     DASTKU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447076"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="VFQ61_00195"
     CDS             complement(<1..1449)
                     /locus_tag="VFQ61_00195"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HET9952885.1"
                     /translation="MTSSLSRRAVLKSLGPLAGSALAAGCAPALSTSPCTPESGPAAN
                     PPPPSNSPAAAANTNPAQASSPFPKDFQWGAATAAYQIEGAANEDGRGTSIWDVFSKK
                     PGNTFENQTGDIACDHYHRFPEDVALLKNLGARAYRLSVAWPRIFPQGRGAVNEKGLD
                     FYKRLLDELARAGIAPMVTLFHWDFPQALFEKGGWLSRDSAEWFGEYTHAVVSRLGDR
                     ISTYITLNEPSVYIGLGHMAGKHAPGLHLSRAEGLLAAHNTMRAHGRSVQAIRAASKA
                     PVQIGHAVAFWGAHPLTNSPGDLEAARLATFSFLDDPFISNSWWMDPTLTGRYPEQAT
                     GFLRADMPKGYEQDLALIHQPVDFMGLNLYGSNQMRQRAGGGVEYVPYPAGFPRAAND
                     WQPLVPQALYFGPRFAYERYRLPIHITENGLSVRDQLFLDGTIQDAQRIDYLQRALSE
                     MARALRENIPIRGYFHWSLLDNFEWADAYKQRF"
     gene            complement(1474..2442)
                     /locus_tag="VFQ61_00200"
     CDS             complement(1474..2442)
                     /locus_tag="VFQ61_00200"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9952886.1"
                     /translation="MSTEAPLSGAPQESSSVGVPLLLFLATVVSVFHAGAYFAGVEPS
                     GGFISSFVDLKFLSRGASFGVPLLAILVTHEFGHYIAARIHRVPASLPHFIPLPGLGT
                     GTLGAVISMRGRIRSRSALMDIGASGPLAGLCVALPVLVFGLRHSEVLPQLASGYTQE
                     GQSLGYLALKYLVLGPIPAGYDVQLHPTAFAGWVGLLVTMINLLPWGQLDGGHVAYAL
                     LGEKHHRIAYWLRRSLLLLFGYNLFRFVLPVLLGHSQLGLFLAISNSMFWLVWFLVLG
                     LIARASAGEAHPPFEPGPLSLARRIIGVVCLVTFVLLFMPTPVASF"
BASE COUNT          556 a          762 c          762 g          414 t
ORIGIN      
        1 gaaacgctgc ttgtaagcgt ctgcccactc gaaattgtcc agcaaggacc agtgaaagta
       61 gccgcggatg gggatgtttt cacgcagcgc tcgcgccatc tcactgagcg ctcgctgcaa
      121 gtaatcgatc cgttgcgcat cctgaatcgt gccgtccaag aagagttggt cgcgcacgga
      181 aagcccgttc tcggtaatgt gtatcggtaa gcgataccgt tcgtaagcga agcgcgggcc
      241 aaaatagagt gcctggggaa cgagcggctg ccagtcgttg gccgctcggg gaaaaccggc
      301 cggatagggg acgtactcca cacctccccc ggctcgctgc cgcatttgat tggagccgta
      361 gagattcaaa cccatgaagt ccaccggttg gtggatcagc gccagatctt gctcatagcc
      421 cttcggcata tccgccctaa gaaatccggt agcctgttcg gggtaacgcc ccgtcagcgt
      481 gggatccatc caccatgagt tcgagatgaa cggatcgtcc aagaaggaga acgtggccaa
      541 gcgtgccgcc tccagatctc ctggactatt cgtgagcggg tgcgctcccc aaaaggctac
      601 cgcatgcccg atttggacgg gtgctttcga ggcggcccga atcgcttgca cggagcgccc
      661 atgagcccgc atcgtgttgt gggcagcaag caatccctct gcgcgcgaga ggtggagccc
      721 tggcgcatgc ttgcccgcca tgtgcccgag cccaatgtag acactgggct cgttcagggt
      781 gatgtatgtc gagatccgat cccctagacg ggacacgacc gcgtgggtgt actcaccaaa
      841 ccactcggcg ctgtcgcgcg agagccagcc acccttctcg aataatgcct gcgggaagtc
      901 ccagtggaac aacgtcacca tcggcgcgat gccagcgcgc gcgagctcgt cgagtaggcg
      961 cttgtaaaag tccaagccct tctcgttcac ggcgccgcgg ccctgcggaa aaattcgtgg
     1021 ccaagccacg ctgagccggt acgcgcgtgc gccgaggttc ttcagcagcg cgacgtcttc
     1081 gggaaatcga tgatagtggt cgcaggcaat atcgccggtc tgattttcga aggtgttgcc
     1141 cggcttctta ctgaagacgt cccagatgga agtcccgcga ccgtcttcgt tggccgcgcc
     1201 ttcgatttga taggcggcgg tcgcagcccc ccactgaaaa tccttgggga aagggctcga
     1261 ggcctgcgcc ggatttgtat tcgctgctgc ggctggagaa tttgagggcg gagggggatt
     1321 cgcggccggg ccggactcag gtgtacaggg agatgtggac agagccggcg cgcagcccgc
     1381 cgcaagcgcg ctgccggcga gtggacccaa tgatttcaga accgcgcgtc ggctgaggga
     1441 cgaagtcatg cccccgcaga gtagcccatc tattcagaaa gaggcgaccg gtgtgggcat
     1501 gaacaacagg acgaacgtca ctaggcaaac cacgcctatg atccgacgag cgaggctgag
     1561 tgggccgggt tcgaatggag gatgggcctc accggccgac gcccgtgcga tgaggcccag
     1621 aactagaaac caaaccagcc aaaacatcga attggagatc gccaaaaaca gacccaattg
     1681 cgaatgcccc agcagcaccg gcagcacgaa ccgaaacaga ttgtacccaa acaacaagag
     1741 caacgagcgc cgcagccagt acgcgattcg gtgatgcttc tcgccgagaa gcgcatacgc
     1801 cacgtggccg ccatcgagtt gcccccacgg cagcaggttg atcatggtga cgagtaaacc
     1861 cacccaaccc gcgaatgcag ttggatgcag ctgaacatcg tagcctgcag gaatgggccc
     1921 gagcaccagg tacttgagcg ccagatagcc gaggctctgg ccctcctgtg tgtacccgct
     1981 cgcgagctgg ggcaaaacct cggagtgacg cagcccaaag accaatacag gcagcgcgac
     2041 gcacaggcct gcgaggggcc cggaggcacc gatatccatc agtgcgctgc gtgagcggat
     2101 gcgaccgcgc atcgagatga cggcacccag ggtgcccgta ccaagcccgg gcaatggaat
     2161 gaagtgcggt aatgacgcgg gcacgcggtg aatgcgcgcg gcgatatagt gcccaaactc
     2221 gtgcgtcacg aggatcgcca gcagaggaac gccaaacgac gccccacgac tcaagaactt
     2281 cagatcgacg aaagagctga taaacccgcc cgacggctcc actccggcaa aataggctcc
     2341 cgcgtgaaag acactcacca ccgtagcgag aaatagaaga aggggcacac cgaccgagct
     2401 gctctcttgc ggtgcgcccg acagcggcgc ttccgtactc atctcgtctt attcagacgc
     2461 gttctacgac aaccgccact gcttcccctc cgcc
//
LOCUS       DASTKU010000008         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_670054, whole genome shotgun sequence.
ACCESSION   DASTKU010000008 DASTKU010000000
VERSION     DASTKU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670054"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFQ61_00205"
     CDS             <1..720
                     /locus_tag="VFQ61_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952887.1"
                     /translation="WPSRCERPHGVARSGEQLRGRAVEASRAANDPIRMEVSDPSERP
                     PTFVMRGTPQGPHRLVFLHGMCGHGLGYAQAFQFAASKYGTLIAPQGDVSCGGVWSKW
                     SMNLDALDERITRTFRALGHEEPIGDVLVLGYSQGASRAEALARKWPQKYTRLVLMGG
                     PTAPSARGLSHLLAAVTMAGERDRKDLMRLGAQRLKAAGVPTEFRVIPEATHGAMGPT
                     PERTMGDVLSWIYSQPEADPG"
     gene            complement(722..952)
                     /locus_tag="VFQ61_00210"
     CDS             complement(722..952)
                     /locus_tag="VFQ61_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952888.1"
                     /translation="MQPNQPVPENPEGQRLDRKPPGKHGSPKLPFEPGGADEQSSKSD
                     FPGQADQRTADDKDGNSEQARHNPANAPNRGR"
     gene            complement(995..1405)
                     /locus_tag="VFQ61_00215"
     CDS             complement(995..1405)
                     /locus_tag="VFQ61_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952889.1"
                     /translation="MPTQQANNSKQSAKQPAGGRRPPATASAPAPKQNRTASSPSGAA
                     ERDETYGLISVLYHALQGAETYSQYIADAERAGKTELVEFFRECQEEENARAKRARSL
                     LADALDEADDEDVEDEDEDKDEDEDEDENEDEED"
     gene            complement(1453..1617)
                     /locus_tag="VFQ61_00220"
     CDS             complement(1453..1617)
                     /locus_tag="VFQ61_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HET9952890.1"
                     /translation="MLHWSIALFVIALIAAFFGFGGVAAGAAGIAKILFFVFLVLAVV
                     SLIFGRRAPV"
     gene            complement(1782..2225)
                     /locus_tag="VFQ61_00225"
     CDS             complement(1782..2225)
                     /locus_tag="VFQ61_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952891.1"
                     /translation="MSANPSPRPHPFPAWMLCLLCAACSGSLPEPPKTQHPNEAYDEV
                     PYPPPAALAETVPPRPDQRGVVWLEGDWVFRGATYAWVRGGWTIAPEKARYAVSNIVY
                     TRDGRILFAPAAWYDSNGQKLERVRPIAPAKTPSNEYTSETQTAR"
     gene            2355..>2632
                     /locus_tag="VFQ61_00230"
     CDS             2355..>2632
                     /locus_tag="VFQ61_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HET9952892.1"
                     /translation="MTALACAPIPKGRYTLETVHMSGDHAVDDDEIAAVIASKPSPRF
                     LGLFSGVIYDYEIFDRYVLERDLQRIERYYRARGYYEARVRAARVIYKG"
BASE COUNT          435 a          802 c          848 g          547 t
ORIGIN      
        1 tggccttcgc gctgcgagcg accacatggc gtagctcggt cgggggagca actccgaggc
       61 cgtgcggtcg aagcttcgcg cgctgcgaac gacccgattc gtatggaggt ttcggatccg
      121 tccgagagac caccaacgtt cgtcatgcga ggcacccccc aggggcctca ccgtctggtt
      181 tttctgcacg gtatgtgcgg tcacgggctc ggatacgcac aggcctttca gttcgcagcg
      241 tcgaagtatg ggacgctgat cgctcctcaa ggcgacgtct cgtgcggagg tgtctggtcc
      301 aaatggtcga tgaacctcga tgcgctcgac gaacgcatca cgcgtacgtt tcgagcgctc
      361 gggcacgagg agccgatcgg ggacgtgctg gtgctcggct attcacaggg tgcttcacgt
      421 gccgaagcgc tcgccagaaa gtggccgcag aagtacacgc gactggtcct gatgggcggg
      481 cccacggctc cctcggcgcg gggcctttct catttgctcg cagccgtcac gatggcgggc
      541 gagcgcgatc ggaaagactt gatgcgcctc ggcgcgcaac gcttgaaagc cgccggtgtt
      601 ccgacggagt ttcgcgtcat tccagaagcg acccatggcg cgatggggcc tacccccgaa
      661 cgcaccatgg gtgatgttct gtcctggatt tattcacaac cagaagccga tccggggtga
      721 atcagcgacc gcggttcgga gcgttcgcgg gattgtgccg cgcctgttcg ctgttgccgt
      781 ccttgtcgtc agccgtgcgc tggtcggctt gtcctggaaa atccgatttc gaagactgct
      841 cgtccgcgcc gcccggctcg aacgggagct tggggctgcc atgctttccc ggcggcttgc
      901 ggtcgagccg ctgcccttcg ggattttcgg gaacgggttg attgggttgc attgattcac
      961 ctcgttcgaa gaggagaggg cgagcgggac tcgctcagtc ctcctcgtct tcgttctcgt
     1021 cttcgtcttc gtcttcgtct ttgtcctcgt cttcgtcctc tacgtcctcg tcgtccgcct
     1081 cgtccaacgc gtcggcgaga agcgagcgag cgcgcttggc gcgtgcgttc tcttcctctt
     1141 ggcactcgcg gaagaactcc acgagctccg tctttcctgc ccgctcggcg tcagcgatgt
     1201 actgcgagta cgtctctgct ccctgaagcg cgtggtagag cacgctgatg agcccgtagg
     1261 tctcgtcgcg ttcggccgcg ccgctcgggc tagacgcggt acgattctgc ttcggggcag
     1321 gagctgacgc cgttgcgggt ggccgacgac cgcccgccgg ttgcttcgcg ctctgcttgg
     1381 aattgttggc ttgctgcgtc ggcatcgtta gaacctccaa atactcgtgc gtctacggat
     1441 gtggcgtttg aatcagactg gggcgcgacg gccgaagatg agagacacga ccgcgagcac
     1501 caagaacacg aagaacaaga tcttggcgat gccggcagcg cccgctgcga cgccaccgaa
     1561 accgaagaag gccgcgatca acgcgatgac gaacagtgca atagaccagt gcaacatgat
     1621 gtcctccaac attcgcagcg atcgttggct gcgtgctcgt gatgtagcga agctcgtgcc
     1681 agcgctgcgg cggtgtctcg aaccagggcg ttgaacgcac gcccagagcg aggctgtggc
     1741 gaattgcctc gacatggaca cctttgtcgg cacgcgtttg ctcagcgagc ggtctgcgtc
     1801 tccgacgtgt actcgttcga cggcgtttta gccggagcta tggggcgcac gcgttcgagc
     1861 ttttgcccgt tggaatcgta ccaggccgca ggagcaaaca ggatccgccc gtccctcgtg
     1921 tagacgatgt tcgaaacagc gtagcgcgct ttctccgggg ctatggtcca gccgccacgg
     1981 acccaagcgt aagttgcgcc tctgaacacc caatcgcctt ccagccacac cacgccgcgt
     2041 tggtcggggc ggggcggaac cgtttccgcg agcgctgcag gcggcgggta cggtacttcg
     2101 tcgtaagctt cgtttgggtg ttgcgttttc ggcggctcgg ggagggagcc gctacacgct
     2161 gcgcacaaca ggcacagcat ccacgctgga aaaggatgag gtcgagggga agggttcgcg
     2221 ctcatgacgc cattcgttcg tcgggttgga acgccgattg cagaacgcat gccgtgtcgc
     2281 gtccggcacc ctgccgcttt cttaaatcgt tggtctctct gcgttcgaca tggtcgctca
     2341 ttttgctgct cggattgacc gcgctcgctt gcgcgcccat tccgaagggc cgctacacgc
     2401 tcgaaacggt gcacatgtca ggagatcacg cggtcgatga cgacgagatc gcggcggtga
     2461 ttgcaagcaa accgagcccg cgcttcctcg gcttgttctc gggcgtgatc tacgactacg
     2521 agattttcga tcgctacgtg ctcgagcgcg acctgcagcg aatcgagcgt tactatcgag
     2581 cacgcggcta ctacgaagcg cgcgtgcgcg ctgcgcgggt gatctacaag gg
//
LOCUS       DASTKU010000009        12938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1562014, whole genome shotgun sequence.
ACCESSION   DASTKU010000009 DASTKU010000000
VERSION     DASTKU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12938
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562014"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFQ61_00235"
     CDS             complement(<1..330)
                     /locus_tag="VFQ61_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952893.1"
                     /translation="MKIGDCIAGRFRIEARAASGGMGTLFHAIDQSTLEAVALKTWLP
                     TQPPAPQATDADSSDRLDSLFEREARALCRVEHPAVVRYVAHGISAEREPYLVMGWVP
                     GEDLAHRL"
     gene            complement(317..1741)
                     /locus_tag="VFQ61_00240"
     CDS             complement(317..1741)
                     /locus_tag="VFQ61_00240"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9952894.1"
                     /translation="MSHAAETSEDSQRVPRLDHRARPGLVLIWNRARPTCSVLPLQNS
                     KLELGRAHQPELFGDDERVSRLHCLLEFSSSEFWLTDRESRNGTYLNGERIHARTQVT
                     GRALIRIGQSLAMAVHDVRPYATEPLVDGAGVVLGAALRQAWLEIELAAHAGSTLYLR
                     GESGSGKELAARAFHDRATRGVSGAPFIAVNCAAVPEGLAERLLFGAKRGAYSGATTD
                     VEGYVQAAHRGTLFLDEIAELEPKVQAKLLRVLESREVLALGATRAQAVDLRVCVAAH
                     QSLRELVSSGRFREDLFYRLARPEVRLAPLRERLDELPWLIWGEVARVLDEAGTTSAS
                     PSTMDSTQSPLTSTLLIEACALRHWPGNVRELKAEIRRAAHRAIRAGAPRVGEEHLDA
                     EAGQPLRAEAQAEEHEGMQKANIDDVRIREVLTECGGNVSQAARVLGWHRNQLRRWLA
                     KQASPNPDAESPTLAAAAQEHEDR"
     gene            2018..2722
                     /locus_tag="VFQ61_00245"
     CDS             2018..2722
                     /locus_tag="VFQ61_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9952895.1"
                     /translation="MQRTIVVGDVHGCLRELEDILKESAWSRGENLILAGDLVAKGPD
                     SAGVVALAREYGARAVLGNHDAHVLRLRAIDLGREPDDGKRAKPEHQQVLDTLSSKDF
                     EYLESLPLLVNLGPEQSDPANPVPADTIVVHAGLAAGVALEAQERDNLLNMRSIREDG
                     RITKKITGRPWAELWPGPQRVVFGHDAIRGLQQHRYATGLDTGCVYGRRLTALLLPER
                     RLISVPARREYVSIEG"
     gene            complement(2779..2863)
                     /locus_tag="VFQ61_00250"
     tRNA            complement(2779..2863)
                     /locus_tag="VFQ61_00250"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2827..2829),aa:Leu,seq:caa)
     gene            complement(3033..3962)
                     /locus_tag="VFQ61_00255"
     CDS             complement(3033..3962)
                     /locus_tag="VFQ61_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9952896.1"
                     /translation="MSRTVLLATEKPFSASARNEVVAIIEQAGYTARVLESYKGKAPL
                     IEAIADADAAIVRSDIIDAEVLAGASKLALVVRAGAGYDNIDCAAAKAKNVAVMNTPG
                     QNANAVAELVFGMLVYMARGKFNGKTGAELRGKTIGLHAFGAVGRAVAAIAKGFGMRT
                     LAFDPFVKAEQIQQGGAEAVSSVEELYKGSEYISLHIPATAQTKGSIGKQLLSLMPKG
                     ATLVNTARKEVINEAELLEVFEARKDFRYVSDIAPSDATAAALKEKFADRVYFTPAKM
                     GAQTEEANVNAGLAAARQIVGFFERGEKQFIVN"
     gene            4555..5142
                     /locus_tag="VFQ61_00260"
     CDS             4555..5142
                     /locus_tag="VFQ61_00260"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET9952897.1"
                     /translation="MNFEVAPLPYAKNALEPVMKQETLEFHYEKHHKGYMTKLKGLIE
                     GKPDAQKSLVELIRTSSGGIFNNAAQVWNHTFFWDGMKPGGGGAPPAGDVGDLVQSYG
                     GWDKLRTDFINGGAGRFGSGYVWLVLDGGKAKILDTPNAETPLTTNQVPLLTADVWEH
                     AYYLDHRNNRNAFLEAFLDKLVNWEFVAKNLRAAK"
     gene            complement(5373..5846)
                     /locus_tag="VFQ61_00265"
     CDS             complement(5373..5846)
                     /locus_tag="VFQ61_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952898.1"
                     /translation="MVDLPDPGLYRTTQPMPGHESEFPSEALVYVGQSQNGGGKFVVR
                     PGDNRRNRWFWRDPTTPLRSPSWARTLKPLPSEGFYTLPDTVEFEGGGRWLKNAIVQL
                     GYNEKGQGILFVGEWREEGEENALHFSDRGLLIQDNLLERLNWAPILPVKPKTNA"
     gene            6089..6877
                     /gene="bcp"
                     /locus_tag="VFQ61_00270"
     CDS             6089..6877
                     /gene="bcp"
                     /locus_tag="VFQ61_00270"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HET9952899.1"
                     /translation="MKKVSKTASPKGSLSAKGKAGSPRSSGKPASNAGKRTAPLKVVP
                     GKAAAEKSTSEKSTSPKGTAERTTGRKPATVTRLPAKLTSVGKKKASEPRAPSSKAAE
                     LTEVEALPGARVDVGDAAPSFVLKDDSQAVVSSEAFRGAPYVLYFYPKDDTPGCTLEA
                     CGFRDLMSRFSAKGVKIVGVSADSPESHQRFKKKYSLNFPLISDPEKTLAKAYGVWVK
                     KKNYGREYMGIERSTFVIDRTGHIKHVFRSVRVPGHVEAVLDLV"
     gene            complement(6971..7777)
                     /locus_tag="VFQ61_00275"
     CDS             complement(6971..7777)
                     /locus_tag="VFQ61_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9952900.1"
                     /translation="MDEVSMRATKDSFFAMNSLVSPRDLHVVFQPIVHLETGEPFAQE
                     ALLRCRSPKFNGPLELFEHAMQAGCVGRLGRLVRELAVPLVQGTPLFVNIHPQELQEG
                     WLVRPDDPIFAHDERIFLEITESVPLSHYDLCIDVLKDMRNRGIDLVVDDLGAGYSNL
                     KRIADLEPRVVKLDRDLICGLDRKERQRRLVTSVVRLCTDLQAVVVAEGIETEDELKA
                     LIDTGAHYGQGFLLARPSYPVPNVTWPPTTQRASVNAIEKSRTRRRASAG"
     gene            8434..9381
                     /locus_tag="VFQ61_00280"
     CDS             8434..9381
                     /locus_tag="VFQ61_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952901.1"
                     /translation="MKPLLTPRSQPVPWSIRTSSGSVVTGSVVAPGAECGRRRVLAGI
                     GSLGLVGLFANCKKRSAESAEKAKSDVAFLARAVASDVKEVRSGLPVGAGHMQEFFSE
                     GKLDDAQAARAALERARGKVQDLRVAKSTFFAAVDPNGKVLRTDQEQDSLAGKNLFTS
                     FPELRGAVSGYVETRGAMAEASEIRGRPDAQWVAGCPVRVGDATKGVYATGWSFSAYA
                     YRLENQLRSTLRSQLKDREKEPLIYVFVIVAGDVYAAPVSPDVNVRAVKERRLLESGT
                     AAQPATLELEIEGREFGLAFLRTEPLGKDVGVAVLRSET"
     gene            complement(9512..9727)
                     /locus_tag="VFQ61_00285"
     CDS             complement(9512..9727)
                     /locus_tag="VFQ61_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952902.1"
                     /translation="MSDSAPAARRPGEPKTRTLIQAGAVLTVFGIALSAAGDGSIGRW
                     LTLGGMFVLIVGLHRFGRLGPDEPKQR"
     gene            complement(9724..10242)
                     /locus_tag="VFQ61_00290"
     CDS             complement(9724..10242)
                     /locus_tag="VFQ61_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952903.1"
                     /translation="MPCEASLRPVYRAILLLLLSASAACSRPSPRAANPTRVLDERRA
                     VQVIAETFAEESATPGSGPDLELAPLRRLQVDVWANGRRFGVAYLTQKEREGLRDVLP
                     ARDPKMGDALQLVSGIGGDADARVCVLYDSDYLYDDLVGESHEKSTLTAELKLKRDVR
                     DFLAITRRENMK"
     gene            10658..11194
                     /locus_tag="VFQ61_00295"
     CDS             10658..11194
                     /locus_tag="VFQ61_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952904.1"
                     /translation="MKLYAEAARQGDARRIYALLSERSQHELGLSGTQRLVQDAKGEL
                     AAQGQGLARADVVLEARAVIRYDDGETASLTLDSGRFWIDSAAALPLQPRTPAEALAE
                     LRRALARRSYAALVRLLSRDTQAALENQVGSLVEGLESPETLDVKADGDSAEVELSGG
                     HWVKLKREAGIWHVEDVR"
     gene            11194..11964
                     /locus_tag="VFQ61_00300"
     CDS             11194..11964
                     /locus_tag="VFQ61_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HET9952905.1"
                     /translation="MIVTRRRALWCGASLATAALHGTRDAGAFGESGAFNPRLLVSGG
                     RKLAGSPRFAGAGRWSMELIRRTSAPARQVVSEVLADRPELWSEPFAVWAGDSDIGSL
                     SNAELRGLSRFFKLGGLLLVDDSAPGTGAFGRAARRELARVLPEAPPVHLDSSHVVFK
                     SYYLLDRPVGRVLGSSRLEAIARGKTLQVIFSDVDLLGALAHDGQSFTLEVQPGGAQQ
                     REYAIRLAVNLAMYVLCSDYKDDQVHASWLMRRRPGRR"
     gene            11964..>12938
                     /locus_tag="VFQ61_00305"
     CDS             11964..>12938
                     /locus_tag="VFQ61_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952906.1"
                     /translation="MNTGFSVAEDLGGTILFAIGAVLVLALGGLIFELLRRRVGRITL
                     LLSGLGALLALGLAMLRPVRVSSRESHVGPRVVVLLDRSRRLLLPADKGHTREDQLRQ
                     VVQTIGRTFSSARLGWMQFGRSEPSPSSPKTLPVPRDDDSDLTAALAWLERASQERPD
                     AVIVVSDGRFTRPPEAALDLDLARSVSQLGVPVHTVSLSDFTPRDAALRAVQNVGAAV
                     AHQPLTLRVGVACTGGLSCADVPVQVRELRQAEAPAVLAEGVAQLEGRETRVIELPIT
                     LERAGPRIVEVSLRAPEGDQIPENDRRLLALNVVRDRVRLLHVAGRPTY"
BASE COUNT         2355 a         3964 c         4147 g         2472 t
ORIGIN      
        1 caaacgatgc gcgagatcct cgccgggaac ccaacccatg accaggtagg gctctcgttc
       61 ggcgctgatg ccgtgcgcca catagcgaac gaccgccggg tgctcgactc ggcacaatgc
      121 ccgcgcttct cgctcgaaca gcgaatcgag tcgatcggag gagtcagcgt cggtggcctg
      181 gggcgcgggc ggttgagtcg gtaaccaggt cttcaacgca acggcttcga gcgtactctg
      241 atcgattgca tggaacaggg ttcccatgcc ccctgacgcg gctcgcgcct cgattctgaa
      301 gcgccctgcg atgcaatcac cgatcttcat gttcctgcgc tgcagcagcc aaggtcggcg
      361 attcggcatc cgggttgggc gacgcttgct tcgcgagcca ccgtcgtagc tgattgcggt
      421 gccagccgag cacacgggcg gcctggctca cgtttccccc acactccgtg agcacctcgc
      481 ggatgcgtac gtcatcgatg ttggccttct gcattccctc gtgctcttcc gcctgggctt
      541 cggcgcgcag aggttgcccc gcctctgcgt ccaaatgctc ttcaccaacc cgtggtgccc
      601 ccgcacgaat cgcgcgatgc gcggcgcgcc ggatttcggc cttcaattcg cggacgttcc
      661 cgggccagtg gcggagcgcg caggcctcga tcaggagcgt ggaggtcagg ggagactgcg
      721 tcgaatccat ggtgctcggc gatgcactcg tcgtccccgc ctcgtccaaa acgcgcgcca
      781 cctcacccca aattagccag ggcaactcgt ccagacgttc gcgcagggga gccaggcgca
      841 cttctggtcg cgcgagccga tagaacagat cttcacggaa ccggccggag ctcacgagct
      901 ctctcaaact ttggtgggcg gcgacgcaca cgcgcagatc taccgcttgg gcccgtgtgg
      961 ccccgagcgc cagcacctcc cgactctcca gaacgcgcag cagtttggcc tgcactttgg
     1021 gctcgagctc ggcaatctcg tccaaaaaca gcgtgcctcg atgcgccgcc tgcacgtagc
     1081 cttcgacgtc cgtcgtggca cccgaatagg cgccgcgctt ggcgccgaac aacagtcgtt
     1141 cagccaagcc ctcggggacc gccgcgcagt tgacagcgat gaacggcgct ccgctcacgc
     1201 cgcgcgtggc ccgatcgtgg aaagcgcgcg cagccagctc tttgccggag ccgctctccc
     1261 ctcgaaggta caacgtactc cccgcatggg ccgcgagctc gatctcgagc caagcctggc
     1321 gcagcgctgc accgagcacg actccagcgc catccaccaa cggctcggtg gcgtaggggc
     1381 gtacatcgtg aacggccata gccaagcttt gcccgatgcg gatcaacgcg cgcccggtga
     1441 cctgtgttcg cgcgtgaatt cgctcaccgt tcagatacgt cccgttgcgg ctttcccgat
     1501 cggtcagcca gaactcactc gagctgaact cgagcaaaca gtgcagacgc gagacccgct
     1561 cgtcatctcc gaagagctcg ggttggtggg cccttcccag ttcgagcttc gaattctgta
     1621 acggcagtac gctgcacgtg gggcgcgccc ggttccaaat caggaccagc cctggtcgtg
     1681 cccggtgatc caagcggggg acccgttggc tgtcctcact ggtctcggca gcgtggctca
     1741 ttgggcccta tcctagcctg gaatcgccac ccatcccggt cgggatgcgc agtgatcgag
     1801 gcgccatgcg tcggctacgg agttgagcag aatttcgatg gcgcgcggga gcgtctcgaa
     1861 gcggcgcctg ccaaggtcgc ctcggttcag tgcggcgagt ccgtgcccaa ttaccccgcg
     1921 gtccccagcg gcggaagcgc gcctcggctg gtccctcgat tggccgcgca ggtccgcggt
     1981 ggcgtaccca gcgggttcgg gcaatgctcg acacgagatg caacggacga tcgtcgtcgg
     2041 cgacgtacat ggctgtctgc gcgaactcga ggacattctg aaagagagcg cgtggagtcg
     2101 cggcgaaaac ctgatcttgg cgggagattt ggtggccaag ggcccagact cagcgggcgt
     2161 agtggcgctg gctcgcgagt atggtgcgcg ggcggtactc ggaaaccacg acgctcacgt
     2221 gctccgcctg cgcgccatag acttggggcg ggaacccgac gacgggaagc gggcgaaacc
     2281 cgagcaccag caagtgctcg atactctgtc gtccaaggac ttcgaatacc tcgagagcct
     2341 gcccctactc gtgaatctgg gtccagagca gtcggacccc gcgaatcccg tgcccgccga
     2401 taccatcgtc gtgcacgcgg ggctggccgc aggcgttgcg ctcgaagcac aggagcgaga
     2461 caacctgctg aacatgcgca gcatccggga ggacgggcgc atcaccaaga agatcaccgg
     2521 ccgtccctgg gctgaactgt ggcccggtcc tcagcgagtg gtcttcgggc atgacgcgat
     2581 tcgcggcctg caacagcacc gctatgcaac ggggctcgat acgggttgcg tctacgggcg
     2641 ccgcctgacg gcgttgctgc ttcccgaacg caggctcatc tcggtgcctg cacgtcgcga
     2701 gtacgtttcg attgagggct gagcgtccgc tcgagacaac gagtgcagca gggcaacctg
     2761 cgctcgtaga gtttcttgtg ccgaaggtgg gagttgaacc cacacgtcat ttggtgacaa
     2821 cggattttga atccgccgcg tctgccggtt ccgccacttc ggcttgggtg tgttcttgcg
     2881 cgagcctctt agcaggcctc gaggaaacgg ggtagcaaaa gttacttggg ggagaggttt
     2941 gtgtgtcgcg agccctgcaa ccctcggcac agacgaggtg gtgaagccgc gtgctctcca
     3001 ccacctccca ggctcccctc agcagcgagc tctcagttca cgatgaactg cttctctccg
     3061 cgctcgaaga atcccacgat ttgacgagcg gcggccaggc ctgcattcac gttcgcttcc
     3121 tcggtctgag cacccatctt cgcgggcgtg aaatagacgc gatcggcgaa cttctctttc
     3181 aaggccgccg cggtcgcgtc gctcggcgcg atatccgaga cgtaccggaa gtccttacga
     3241 gcttcgaaca cctcgagcag ctcggcctcg ttgatcacct ctttgcgggc cgtgttcacc
     3301 agcgttgcac ccttcggcat caacgacagg agctgcttgc caatcgaacc cttcgtttgg
     3361 gccgtggccg ggatgtgcag agagatgtac tccgagccct tgtacagctc ctcgacgctg
     3421 ctgaccgctt cggcgccgcc ttgctggatc tgctcggctt tgacgaacgg atcgaacgcg
     3481 agcgtgcgca ttccgaaacc cttggcaatc gcggctacgg cgcgacccac tgcaccgaag
     3541 gcgtgcagac cgatggtctt gccgcgcaac tcagcgccgg tttttccgtt gaacttgccg
     3601 cgcgccatgt aaacgagcat gccgaacacg agctcagcca ccgcgtttgc gttttggccg
     3661 ggggtgttca tgacggccac gtttttggct ttggcagccg cacagtcgat gttgtcgtaa
     3721 ccagcacctg cgcgcactac gagtgcgagc ttgctggcgc cggcgagcac ctccgcgtcg
     3781 atgatgtcac tgcgcacgat cgcggcgtcg gcatcggcaa tcgcctcgat caggggcgcc
     3841 ttgcccttgt acgactcgag cacccgcgcc gtgtagccgg cctgctcgat aatcgcgacg
     3901 acctcgttgc gggccgacgc ggaaaacggc ttttcggttg cgagcaaaac agttctggac
     3961 actttgacct cgagcgtttg ggaaatgaaa ccgggctgat tccgggaccc cttcactaaa
     4021 tcagtgatcc gcgttcggca acggccaaac gtctgttcgc acacgcatgc gactgttttg
     4081 tcgcgaaagc gacggtcggc ccgtcagttg gcgagccgcc agttcatgca agtacgggcg
     4141 acgggactcg tcttagttcg aacggctcga tcgatgcgcc gtcggcagcg agcacaggag
     4201 ttcgtaaggt tgtctccgct gttgttcggg ttgttcggat ccagacaaag gtacgcgtgc
     4261 gaaaagtaac atcgtcgcac gcgcgctcac cggcgcgacg agattggcgc gttcgttcgt
     4321 accaacgctg cggtcctcgt ctagttcagt cgttcgcatg gagggagccg gcagcgctcg
     4381 aaacacttat ccggcgtggg cggcagaaac atgcctcgtc gggagtgcgg ttgttatgac
     4441 cctctcattg ccagcgccgg aaaccgaata cccagacacc cagcgcattg gggcattgac
     4501 acaaagcata cttggtcgga gattcggagc gcgagatata cagaggagac cgtaatgaat
     4561 ttcgaagtag ctccgctccc ttacgccaag aacgcactcg agcccgtgat gaagcaggaa
     4621 acgctcgagt ttcactatga aaagcaccac aagggctaca tgaccaagct caaggggctg
     4681 atcgaaggaa agcccgacgc gcagaagagc ttggtcgagt tgattcggac gagctcgggc
     4741 ggcattttca acaacgcggc gcaggtctgg aaccacacct tcttttggga cggcatgaag
     4801 ccaggcggcg gtggcgctcc gcctgcgggc gacgtgggcg acttggtcca gagctacggc
     4861 ggttgggaca agcttcgcac cgacttcatc aacggcggcg cggggcgctt cggttcaggt
     4921 tacgtatggc tcgtcctgga tggcggcaaa gcgaagatcc tggatacgcc aaacgccgag
     4981 accccgctca ctacgaacca ggttccgctg ttgaccgcgg atgtttggga gcacgcttac
     5041 tacttggatc atcgcaacaa ccgcaatgcg ttcctggagg cgttcctgga caagctcgtc
     5101 aactgggagt tcgtcgccaa gaacctgcgc gcggcgaagt gagcccattc gcgcagccta
     5161 gccgcagaat attgggctct ccaaacccct aggctctcca aacccctagg ctctccagac
     5221 gctaggctct cgaaaccaag aaggcctcga cggcgcaacc gccggtcgag gccttttctc
     5281 tatcgagcat tcaacccgag ctcagctcgc ttcgattctc gcgagcatct ccgcagctga
     5341 gctcagccct cgttaactca tcgttcggcc gctcaggcgt tggttttggg cttcaccggc
     5401 agaatcggag cccaattcag ccgctcgagc agattgtctt gaatgagcaa gccacggtcg
     5461 ctgaagtgga gagcgttctc ttcgccctct tctcgccact caccaacgaa caagatgccc
     5521 tggcccttct cgttgtatcc gagctgcacg atggcgttct tgagccagcg acctccgccc
     5581 tcgaactcta ccgtgtcggg tagcgtgtag aagccttcgg agggtagggg cttgagagtg
     5641 cgagcccaag acggggagcg cagaggggtg gtggggtcac gccaaaacca acggttgcgt
     5701 cgattgtctc ccggacgaac tacgaacttg ccgcccccgt tttgcgactg acccacatag
     5761 acgagggcct cgctcggaaa ctcgctctcg tgtccgggca tgggttgcgt tgtgcgataa
     5821 agaccgggat cgggtagatc gaccatagac catcctagaa agaatcaatc gggcaagcct
     5881 tttgcgactt tacccgaacg gcgcgggcac acaagctggt agaacggtct gggttgctgg
     5941 taagaagcgg cccaggtgcg gtccggttca gcggcgctcg agggcgcgtg ctttcggcta
     6001 aaagaacccc ttgaacctag gaacccggaa ttgagctcgg tcgcgtggga cccatacaga
     6061 caggagtgca aacggagagt ccatggccat gaagaaagtg tcgaagaccg cgagccccaa
     6121 aggcagcctc tcagcaaaag gcaaggccgg cagcccgcgc agttccggta agccagcctc
     6181 caacgccggc aagcgaactg cgccgctgaa ggttgtgccc gggaaagcgg cagccgagaa
     6241 gagcacgtcg gagaagagca cgtcccccaa gggtaccgcc gaaagaacga cgggaagaaa
     6301 gccggccacg gttacccgat tgcccgcaaa gctgacgtcg gtaggcaaaa agaaggcctc
     6361 tgaacctcgt gcgccgtcca gcaaagccgc cgaactcacg gaagtcgaag ctttgccagg
     6421 tgcgcgcgtc gatgtggggg atgccgctcc cagcttcgtg ctcaaagacg attcgcaggc
     6481 tgtggtttcg tcggaggcct ttcgaggggc tccttacgtc ctctatttct atccaaagga
     6541 cgataccccg ggctgcacat tggaggcgtg cgggtttcga gatctgatgt cccggttctc
     6601 tgccaaagga gtcaagatcg tcggcgtgag cgcggactcg ccggagagcc atcagcgctt
     6661 caaaaagaag tactcgctca atttccctct catctccgat cccgagaaga ccctagccaa
     6721 ggcctacggc gtttgggtca agaagaagaa ctacgggcgc gagtacatgg gcatcgaacg
     6781 cagcactttc gtgatcgatc ggactggcca catcaagcac gtattccgca gtgttcgcgt
     6841 gcccggccac gtcgaagcgg tgctcgatct cgtctgatgg atagagccgc ggctcgatct
     6901 atgaagctac ggctactctc cttctgaggc gtgcgcgtgg ggtccgggcc cacgcgcagg
     6961 gccccggtcc tcagccggca ctcgcgcggc gacgcgtccg tgatttctcg atggcattga
     7021 cgctcgcccg ttgcgtcgtt gggggccacg tcacgttggg aactgggtag ctcggacggg
     7081 cgagcaagaa gccctgaccg tagtgtgcac ccgtatcgat cagcgccttc aactcgtcct
     7141 cggtctcgat gccctctgcg actacgaccg cctgtagatc cgtgcagagt cgcacgacgc
     7201 tcgttaccaa gcgacgctgg cgctccttgc ggtccagccc gcatatcaag tcccggtcca
     7261 gttttacgac gcgaggctcc aaatctgcga tgcgcttgag attcgaatag cctgctccga
     7321 ggtcgtcgac gaccagatcg atgccgcgat ttctcatgtc cttcaggaca tcgatacaca
     7381 agtcgtaatg gctcagcggt acggactcgg taatctcgag gaagatccgc tcgtcgtgcg
     7441 cgaagatggg atcgtcagga cgcacgagcc acccctcctg caactcttgc ggatgaatat
     7501 tgacgaacag aggggtcccc tgcacgagtg ggactgctag ttcacgtacg agacgcccga
     7561 gcctgccgac gcagcccgcc tgcatcgcat gttcgaacaa ttcgagcggt ccgttgaact
     7621 tgggactgcg gcagcgcagc agcgcttcct gtgcgaatgg ctcaccggtc tccaagtgca
     7681 cgatcggctg aaacaccacg tggagatccc gcggggacac gaggctattc atggcgaaga
     7741 aggagtcttt ggtggcacgc atcgatactt cgtccatgtt ttcccgagcg cctaaggcgc
     7801 acatcgacca ctggaacact caccgcaaac ccaccgccgc gctcatacca ggtgcgactc
     7861 agccagtacg acagctctgc atgcgtacga ttgtgggatt cgatatccgc ggggcgcatg
     7921 atgcatgccc caggcggtta cgttttgagc gctatgcccg caaagtgtga tcaagatatc
     7981 acctcggggg acactccaca actcgtctag tggcgagttt cgcagttgtc tggcgaaaaa
     8041 ctcgcgcagg atcgccgctc gaagttcgcc gcgccacgct tgactcaatc gaacgctcgc
     8101 tcagctcgcg ggctcactgg agtaggctgt ttggggtggc ctggttcgga gtggccgggt
     8161 tcggagtggc cgggttcggg cgggctcacg agagtgtagg taacttaacc cggagctcgt
     8221 gtatgatgcg cggagccaca tgcacttcgc catgacagtt gaactgctag cgcgcgacga
     8281 tgccgcgctt actgcgcctg gtttgcctcg ttcgttcatt gagccaaagc acggcgctca
     8341 atcgtatggt cttggctcgc accttttccg cgggcatccc aatttctcgc aggtccaccc
     8401 gcgattggcg accgagaact ccggggtgaa gccatgaagc ctctgctgac cccgaggagt
     8461 cagcccgttc cctggtcgat tcgaacgtcg agtggaagcg tcgtgacggg gagcgtggtg
     8521 gcgccagggg ccgagtgtgg gcgacgcaga gtgttagccg gaatcggcag tctgggtttg
     8581 gtggggcttt tcgcaaactg caaaaagcgc agtgcggaga gcgccgagaa ggcaaagtcc
     8641 gacgtcgcgt tcctggcgcg ggcggtggcc tcggacgtga aggaagtgcg ctcgggttta
     8701 ccggtcggcg ctgggcatat gcaggagttc ttttccgagg gaaagctaga cgacgcacag
     8761 gccgcccgag ccgcgttgga gcgtgctcgc ggcaaagttc aagacctgcg cgtggcgaag
     8821 agcacgttct ttgcggctgt cgatccaaac gggaaggtgc tccgcacgga tcaggaacag
     8881 gatagcctgg ccgggaagaa cttattcacg agcttccccg agctgcgcgg tgcagtttct
     8941 ggttatgtag aaacgcgagg cgccatggcg gaggcctccg aaattcgagg ccgtcctgac
     9001 gcgcaatggg tcgccggttg tcccgtgcgc gtgggcgatg caaccaaggg tgtctacgct
     9061 acgggctggt ccttctccgc gtatgcttat cgactcgaga atcagctgcg aagtacgctg
     9121 cgctcgcagt tgaaggatcg ggaaaaagaa ccgctgattt acgtcttcgt catagttgcg
     9181 ggtgacgtat acgcggctcc cgtttcgccc gacgtgaacg ttcgggcggt gaaagagcgc
     9241 cgcctgcttg aaagtggcac tgcagctcag cccgccactc tggagctcga aatcgaaggg
     9301 cgtgagttcg gactcgcatt tctacgcacc gaaccgctcg ggaaagacgt aggcgtcgcc
     9361 gtattgcgga gcgagactta gaaccatccc agcaggagcg agtcgccggt ggccgatagc
     9421 tcgcggaacg gaaaacagag ccgttcgctc tcggcccgtc tccggtgctc ctactgcgat
     9481 aggttcttag cggtggttga atcgagccca atcagcgttg tttgggctcg tcggggccga
     9541 gtcgtccaaa acgatgcagg cccacaatca gaacgaacat gccgcccaag gtgagccaac
     9601 gtccgatcga gccgtccccg gctgcggaga gtgcgattcc aaagacggtc aggacggcgc
     9661 ccgcctggat aagcgttcgc gttttgggtt cgccggggcg tcgtgcagcg ggggcggagt
     9721 cgctcatttc atgttctcgc gacgtgtgat ggcgagaaag tctcgcacat ctcgcttgag
     9781 cttgagctca gcggtcaggg tgctcttctc gtggctctcg ccaacgaggt cgtcatacag
     9841 gtagtccgag tcatacagca cgcatacccg ggcgtctgca tccccaccga tgccggaaac
     9901 gagctgcaag gcgtcgccca tcttgggatc gcgcgcggga agcacgtctc gcaggccctc
     9961 tcgctctttt tgcgtgagat acgcgacgcc aaaccggcgg ccgttggccc agacgtccac
    10021 ctgtagacgt cggagcggag cgagctccaa atcggggccg ctacccggag tagcgctttc
    10081 ctccgcgaag gtttctgcga tcacctgaac ggcccgtcgc tcgtcgagca ctcgcgtcgg
    10141 atttgctgcg cgtggcgagg gccggctgca ggccgcgctc gcactgagca ggagcagcag
    10201 aatggctcgg taaaccggcc ggagcgaggc ctcacacggc atttcctcgg tgaccttagc
    10261 gaagacgctt tgccttcgct agtcctcagc cctgttctcc agccgctctg ttttgtcgat
    10321 ttcgccagtc cgtgatgttc ttgacggggc gtgttttcac caaccgcgtt cttctgaacc
    10381 aaacacccgg tcgtggtgaa ctgggtcgtc tgggagtggg ccggccccag ggacgatcaa
    10441 cgcggtgccg acgacgtctg ctttgtgtcc ttcggctggg ccgcgttcac cgccagccct
    10501 ccaaattctc cgcgcacggg cattcgctag atgcatcgcg ctggactagt cttcgccagg
    10561 aatgttagcc gccccaccaa gagctcgcgc cgtgtttttg gcagcggttt gtttttcggg
    10621 ctgcggggca gctgctgtcg cggagccgac tgacacggtg aaactctatg cggaggcagc
    10681 acgccagggc gatgcccggc gtatttatgc cttgttgtcg gaacgctcgc agcacgaact
    10741 cgggctgagt ggaacgcagc gtctcgtcca agatgcgaag ggtgagttag ccgcccaggg
    10801 gcaggggctc gctcgtgccg acgtggtgct cgaggcgcgc gccgtgattc gctatgatga
    10861 cggcgagacg gctagtctga ccctggactc ggggcgcttt tggatcgatt cggcggcggc
    10921 gctgcccctg cagcctcgaa ctcccgccga ggcgctggcg gagctacgtc gggctctggc
    10981 gcgaagaagc tacgcggcgc tggtgcgcct gctctcccgg gacacccagg cggcactcga
    11041 gaatcaagtg ggcagcttgg tcgaaggcct cgaaagtccg gaaacactcg acgtgaaggc
    11101 cgacggggac tctgcggaag tggagctttc tggtggccat tgggtcaagc tcaaacgcga
    11161 agcgggtatt tggcacgtgg aggatgtgcg ctgatgatcg tgactcggcg acgagccctg
    11221 tggtgtggtg cgagcctggc cacggctgcg ctgcatggca cgcgagacgc gggagccttc
    11281 ggggagagtg gcgccttcaa cccgcgtctg ctcgtctcgg gcgggcgtaa gctggctgga
    11341 tcaccacggt tcgcgggtgc cgggcgctgg tcaatggagt tgattcgaag gaccagcgct
    11401 cccgcgcgcc aggtcgtctc ggaagtgctc gcggatcgac cggaactgtg gagcgagccc
    11461 ttcgcggttt gggcaggtga ttcggacatc ggcagcctgt cgaacgcgga gctcagaggg
    11521 ctgtcgcgat tcttcaagct cggagggctg ttattggtcg acgactccgc cccaggcacg
    11581 ggcgccttcg ggcgcgctgc gcgccgcgaa ctcgctcgag tattgccaga ggccccccca
    11641 gttcacctgg attcatcgca cgtcgtcttc aagtcgtatt atttgctgga tcgcccggtg
    11701 gggagagtct tgggctcatc ccgactggag gccatcgcgc ggggcaagac gttgcaggtc
    11761 atcttttcgg acgtcgacct actgggcgcg ctcgctcatg acggccagag cttcacgcta
    11821 gaggtgcagc cgggaggggc tcagcaacgc gaatacgcca ttcgcctggc ggtgaacttg
    11881 gcaatgtacg tgctttgttc ggactacaaa gacgatcaag tccacgcgag ctggctaatg
    11941 cgtcggcgtc cgggtcggcg ctgatgaata cggggttctc ggtcgcggag gatctcggcg
    12001 gcacgatctt gttcgcaatt ggtgccgtgt tggtgctggc gttgggcggc ttgatcttcg
    12061 agctcctcag gcgtcgggtg ggacgcatca cgctcttgct cagtggctta ggcgccctgc
    12121 tcgccttggg actcgcgatg ttgcgcccgg tgcgggtctc gtctcgagag agtcacgtcg
    12181 gcccgcgcgt ggtcgtgttg ctcgaccgct cgcgacggtt gctactacct gccgacaagg
    12241 ggcatacgcg cgaggaccag ctgcgccaag tcgttcagac gattgggaga acattttcat
    12301 cggctcgcct gggttggatg cagtttgggc gtagtgagcc gagcccctcg agccccaaga
    12361 ccctgcccgt gccacgcgac gacgatagcg atcttaccgc tgcactcgcc tggctcgagc
    12421 gtgcgagcca agaacgtccg gatgcggtga tcgtggtcag cgacggccgc ttcacgcggc
    12481 cgcccgaggc ggcactggat ctagatctgg cgcgcagcgt atcccagctc ggcgtgccgg
    12541 tgcataccgt gagcctcagc gatttcacgc cgcgcgatgc ggcgctgcgc gcggttcaga
    12601 acgtcggggc ggcggtggct caccaacccc ttacgttgcg ggtgggggtg gcctgcacgg
    12661 ggggactgtc ttgcgcggac gtgccggtgc aagtgcggga gctaaggcaa gcggaagctc
    12721 ccgccgtgtt ggccgagggt gttgcgcagc tcgaaggcag ggagacgcgg gtcatcgagc
    12781 tgccgattac cttggagcgt gccggaccgc ggatcgtgga ggtcagcttg cgtgcgccgg
    12841 agggtgatca aattcccgag aacgaccggc gtctgctcgc gttgaacgtg gtgcgcgatc
    12901 gagttcggtt gctgcacgtc gcgggccgcc ccacttac
//
LOCUS       DASTKU010000010        11924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_893493, whole genome shotgun sequence.
ACCESSION   DASTKU010000010 DASTKU010000000
VERSION     DASTKU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893493"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /gene="nagZ"
                     /locus_tag="VFQ61_00310"
     CDS             complement(<1..950)
                     /gene="nagZ"
                     /locus_tag="VFQ61_00310"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET9952907.1"
                     /translation="MNDELELERQVASLFCVGFSGLDVPSELVRLIERGVRNVILFSR
                     NVGPLPLLKSLIQNIKSRSVEPVAVSVDQEGGRVQRLREGFTRFAPLGELGQTADEHQ
                     AHRVGATLGRELRAVGIDWDFAPVLDVDSNPLNPVIGDRSFGSDPVRVAKLGIALAQG
                     LQSEGVASCGKHFPGHGDTHLDSHLCLPSLDHGLERLERVELVPFRAAVSGGVASIMA
                     AHVLFRALDAEYPASMSEPVIRGLLRERLGYSGLVVSDDLEMKAIVDHYGIDDAVVRG
                     LCAGVDTFLICHSSERCERAIDAVVRAARNGEVPRARVQEA"
     gene            complement(947..3460)
                     /locus_tag="VFQ61_00315"
     CDS             complement(947..3460)
                     /locus_tag="VFQ61_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9952908.1"
                     /translation="MSERELPRVLRELARLQAAAPWRFIICAVLSLVPAGWAASRLGF
                     KSDFAELLPDNKDSVIEMRRVSARLPGASTLSLVAQIDDGSKRQALRTFVDRLVPELL
                     ALGPEWVGSVDYGVKETRAFFEKNQILYAEVDELRRVRDQVVERYDYEVAKAQGTLLE
                     SEPGEVPPPITRESIEKTLSERKAQAAAQADAYPGGYYESQDGTYIAVLIRTPVSGRA
                     AVDELRRRVEGVVRKLEPKRIDPSMHVDYTGDVITSREEYDAIVRDLGEVGASGVAGV
                     LLSVLLFFWRVRTVLTMGATLLVGLGWTFGLTYWTIGYLNSSTGFLVSIIAGNGINYG
                     IMYMARYVEARRDEALSVEDAVMVAHRDSWIPTLASAATAALAYGSLMVTDFRGFKHF
                     GIIGAYGMLLCWLVTYLFMPPIFAASEHVWPAFKRASGDNRARARGYYGVLFSKLATA
                     APRGTALVGLVLGLASLGLGVRYFLSDPMEYNMKKVRTDDPPNESGARALSHRVDAVV
                     GRLGQEGLAIMTDRLDQVPLLTTELEKRRADAPPDRKPFEKVVSIYSLLPGQQAEKLQ
                     LAEEIRDRLQRARKRGFVQDADWAQIETYLPEKRLSPIGIADLPEQVARPFTERDGTR
                     GRIVYIAPKSGESVWDAKYLMRWADSFRHVQLPNGEIIKGSGRAVIYADMIRTIGEDA
                     PKAITLSALGTILVILFAFRGSPLSVGVFLPWLFGISGLVAFLYLKDIKLNFLNFVAL
                     PITIGIGAEYAHNMMQRYRQENGQRLAHIITETGGAVTLCSLTTTIGYMALLMSVNRG
                     IRGFGLAAAVGEITCLAAAVLWLPAVLAWAARRRERQAP"
     gene            3693..4346
                     /locus_tag="VFQ61_00320"
     CDS             3693..4346
                     /locus_tag="VFQ61_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952909.1"
                     /translation="MTQLFALVRLSNRRLATLKGVRVARLYALNMGFGYVLFTLFLHG
                     ASVRRLGQIVQESLSFASWLVCGIAAFAVLRAPRGFDTSWAELGRLRGYTSAHLQKAY
                     WLSLGLRLALDLVIAVFPALLVIALLADAPTLGQRVGWPLLGGLYAASLGLAIAGLVV
                     LAQRATRDHAVSLFLSLMIAPELLAIFTTLPGFGLPGLFSRALDWMFVIAPLRLGVG"
     gene            4343..5251
                     /locus_tag="VFQ61_00325"
     CDS             4343..5251
                     /locus_tag="VFQ61_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952910.1"
                     /translation="MSTLKLFALEEPDFGPLSLELGLGVSVIVDERAPRLGRLAEILA
                     GVRRSRGGRALLGERDLYTTREARASTWSLLPREEFTARDSEETVARVLGDIARWKGD
                     HEPIEPRLDQAGLGRYAPARAAELDPEQCRALMLALALTARAPEALILHDPLACVPWL
                     EEAFIVQRCRAQAQLCPVLVTTSALLDAARLGGSVSLLWGKNWITPRELVAPTPSWVA
                     VSVRARPIRALANALAERAWITSFRVDAELPDGSSDSAPDAAGVLTLQGHDLAALSAL
                     VVNIALEQGTALELIEPLGRVPYGGR"
     gene            5251..5937
                     /locus_tag="VFQ61_00330"
     CDS             5251..5937
                     /locus_tag="VFQ61_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952911.1"
                     /translation="MLSSSFPTPASLVHEGLLGARAIARRVPLNAALLGWCACFVCAW
                     LTRSRDGGVDDALNVALGLIMPLTAYVSFEGVLDRGRLESAVRGLSRHGLHGKALWLG
                     AAAALLGWLAMISASFAATVVWVSRGVSSDVLLSAWIGAWGGAAYALWFSAASDVGAR
                     GQGRKWLLLVDLTFGSTNGFLALPWPRAHVRNLLGGESWLGLQGPWAFALLFVSGLIA
                     LAIGFRRAPE"
     gene            6031..6744
                     /locus_tag="VFQ61_00335"
     CDS             6031..6744
                     /locus_tag="VFQ61_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9952912.1"
                     /translation="MQGLIYRFETLEQLAQALEASAADRDLPVPCRKGVTEGEFVLVT
                     LSAADEATSVVARVREGGRGLRLSFEQRDWELLERFAQGGGRPTLPPPCGTANPEPVR
                     APAGATALLVDTDASVLSIVRAMLEACDVATQQAHGAEDALNLLRRRHFDLVVVEPAL
                     DGMSGLEMCRRLRADRRLAGVPILVLGSHTTDHDLREALCSGADDFVAKPFRAHELRA
                     RALGLIQKGRALAASVRPA"
     gene            complement(6774..8204)
                     /gene="rdgC"
                     /locus_tag="VFQ61_00340"
     CDS             complement(6774..8204)
                     /gene="rdgC"
                     /locus_tag="VFQ61_00340"
                     /inference="COORDINATES: protein motif:HMM:NF016281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HET9952913.1"
                     /translation="MSARRGSLTLVRYFVDGQPPRDLRKRYLEAVRLRAFQPLKPEDE
                     ATEATGWCVMERPLDLEFELDKLFNDRLLQLGLRVDKFRIPSSAINAELADEERKLLT
                     KTGAEKISRQQRKELKKRVVVKLRRKLVPISRAFDLCWDLDAGVVFFFSHSRRLGEEL
                     CALFEKTFQLRLIEDSPYVVAKRAKLPATILKALDRVMPVSYVNGRKQLGLPDAAPEP
                     ATALVAGSPSPKPRPADPVDAEPTSETGADEGSRAGAEKKPPAAKKAASDKAGGAEKV
                     DPEEDALSTRVETTRFLGPEFLLWLWLRAELTQGPFSLGEHGEYEVWLDRQITLQSAL
                     DANERVTVRGSAPSDSSEAREAVKAQKFPVRARVVMQSTEPEFACVLDALRYALATAQ
                     VPAVLTQEPEQAFLERMELLLRLFAILDGLYAQFLQDRLSDTWSRGYEPALVAWFESQ
                     AVPARVLKHLAKDISTAPQKKRRRSA"
     gene            complement(8264..9856)
                     /locus_tag="VFQ61_00345"
     CDS             complement(8264..9856)
                     /locus_tag="VFQ61_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9952914.1"
                     /translation="MGFPLELALRYMGSKKRAFVSVGTLFAILGVMLGVAALSTVISV
                     TGGFRSQFRDKVLGVNAHVLVLKYSSDFREYRKIMDQMRKVPGVIGVAPFTIKPMMLT
                     HGNRTATGVLLKGVDPVGVSEVLRLPQDIVKGSLAGLRLPAAKPAERRRQSLLDDVPR
                     AASSADDENGPKKTLFQELERLSKTKTDPESASSATPKRPSGTPAEAIEAARAEVEPP
                     PEAPAANGLIGNVEPEGGFKSVLPDDDVLPEELDPDPCSNKNSTAALPGIVVGVTLAK
                     NLGVALGDCIQVTSPTIGFSYSKGSIRAPVAKQFRVIAVFDAGFDQYDSKLAYTDLYE
                     AQAFYDSGDSVDGVEMKVKDIDEARTIAEQIEKKLSNGIYRTMDWEELNHGLFTALRI
                     QQILMSIVLALIIIVAAFTVIATLIMVVLDKKKEIAVLKAMGATDFALLRSFVYQGTL
                     VGMLGTAAGLGLGLLLCKVLLVYGLPLDPKVYFISRLPVQVRAMDFVMVGIFAVLVCL
                     IATVWPALHAARLRPAEAFREN"
     gene            10841..>11924
                     /locus_tag="VFQ61_00350"
     CDS             10841..>11924
                     /locus_tag="VFQ61_00350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9952915.1"
                     /translation="MSEPQDGSPSDGGLVPAVGSAALSSARESVRPGVISSRPPPRIS
                     SRPPAPSGGTRLKIVTVSPRESFPEMEAPTGEREVQRRDPYIGATIDGRYKVEAVIGE
                     GGMGVVYLCSHSIIGKKVAMKVLRADLARDTEVTERFLNEAKAASAIGNPHIIDISDF
                     GQFPDGATYFIMEYLVGTQLSEMLGPGDVVPVPRTISIGRQLAEGLAAAHAAGIVHRD
                     LKPENIFLVTRGAEKEFVKILDFGIAKVSTTSEGKLTRAGAVFGTPHYMSPEQAEGAP
                     VDHRGDIYSLGVIMYEMAVGQVPFDAENFMSILQQHVHKLPVPLRSVEPKLQEVPVEL
                     EAIVLKCLSKRQEDRYQSMHELLSDLD"
BASE COUNT         2112 a         3874 c         3743 g         2195 t
ORIGIN      
        1 gcctcttgca cgcgcgctcg cggtacctcg ccatttcgcg cggctcgcac taccgcgtcg
       61 atggcgcgct cacaacgttc gctcgagtga caaatcaaga acgtgtccac tccggcgcag
      121 agcccgcgca cgaccgcatc gtcgatccca tagtgatcga cgatggcttt catctccaag
      181 tcatcgctga ccaccaagcc cgagtagcca agccgctcgc gcagcaaacc acggatcacc
      241 ggctcgctca tgctggcggg atactccgcg tcgagtgccc gaaacaacac atgggcggcc
      301 ataatcgaag ccacgccccc gctcaccgcc gcccggaacg gtacgagctc cacgcgctcg
      361 agccgttcga gcccgtgatc gaggctcggc aaacaaagat gtgaatccag gtgggtgtca
      421 ccgtgtccgg gaaagtgttt gccgcaactc gcgacaccct cgctctgcag accctgcgcg
      481 agtgcgatgc ccagcttcgc cacgcgcacc ggatcggagc cgaacgatcg atccccaatt
      541 acggggttca gagggttgct atccacgtcc agcacgggcg cgaaatccca atcgatgcct
      601 accgcgcgca gctctcgacc caaagtggcg cccacgcggt gcgcctggtg ctcatccgca
      661 gtttggccga gctcacccaa aggtgcgaag cgcgtgaagc cctcccgcag acgttgtacg
      721 cgcccgcctt cttggtccac gctcaccgcg accggctcca cggaacgtga cttgatgttc
      781 tggatcaagc ttttcagcag cggtaagggg ccgacgttgc gcgagaacag gatcacgttt
      841 cgtacacctc gctcgatcaa tcggaccagc tcggagggca cgtcgagccc ggaaaaaccc
      901 acgcaaaaca ggctggccac ctggcgctcg agctccagct cgtcgttcac ggggcttgcc
      961 gctccctgcg tcgcgcggcc caagccaaca cggccggtaa ccacagcacc gccgcggcca
     1021 agcaagtgat ttcgccgacg gccgcggcca aaccaaagcc gcggatgccg cggttcaccg
     1081 acatcaagag tgccatgtag ccgatggtcg tggtgagcga gcacaaggtc accgccccgc
     1141 cagtctccgt gatgatgtgc gctagcctct gcccgttctc ctgccggtac cgctgcatca
     1201 tgttgtgcgc gtattcggcc ccaatcccga tcgtgatggg cagcgccacg aagttcaaaa
     1261 aattcagctt gatgtccttc aagtagagga aggcgactag ccccgaaatt ccgaacaacc
     1321 agggcaagaa cacgcccacc gagagtgggc tcccgcggaa cgcgaacagg atcacgagga
     1381 tcgtgcccag cgcggagagc gtgatcgcct tcggcgcgtc ttctccgatc gtccggatca
     1441 tgtcggcgta gatcacggcg cggccggagc ctttgatgat ctcaccgttt ggtagctgca
     1501 cgtgacggaa gctatccgcc cagcgcatga ggtacttcgc gtcccacacg ctctcgccgg
     1561 atttgggggc gatgtacacg atgcgcccgc gtgtgccgtc gcgctccgta aaaggtcgcg
     1621 ccacctgctc gggcagatca gcgattccaa tcggggacag ccgcttctct ggcaagtagg
     1681 tctcgatctg ggcccaatcc gcgtcttgca cgaagccacg cttgcgcgcg cgctgcaggc
     1741 gatcacggat ttcctcggcg agctggagct tctccgcttg ttggccgggc agcagtgaat
     1801 aaatgctgac caccttctcg aagggcttgc gatccggggg cgcgtccgcg cgtcgctttt
     1861 cgagctcggt cgtgagcagc ggcacctgat cgagccgatc cgtcatgatc gcaaggccct
     1921 cttggccgag ccggcccacg acggcgtcca cacgatggga caaggcgcgc gcaccgctct
     1981 cgttcggcgg atcgtccgtg cgcaccttct tcatgttgta ctccatcgga tcgctcaaga
     2041 aataacggac cccgagtccg agggaagcga gcccgagtac caagcccacc agcgcggtgc
     2101 cacgcggcgc tgcggttgca agcttggaga acaacacccc gtagtagccg cgcgcgcggg
     2161 ctcgattatc acccgaggct ctcttgaacg cgggccacac gtgctcactc gccgcgaaga
     2221 tgggcggcat gaacaagtag gtgaccagcc aacacagcaa catgccgtag gcaccgatga
     2281 tgccgaaatg cttgaagccc cggaaatcgg tcaccatcag cgagccgtac gccagcgcgg
     2341 ccgtggccgc agacgcgagc gtcggaatcc aggagtcgcg gtgcgccacc atgaccgcat
     2401 cttcgacgct cagggcctcg tcgcgccgcg cctccacgta gcgcgccatg tacatgatgc
     2461 cgtaattgat gccgttgccg gcgatgatgc tgaccaagaa accggtcgag ctgttcagat
     2521 agccgatggt ccaatacgtg aggccgaagg tccaacccaa gccgacgagc agggtggctc
     2581 ccatcgtcag cacggtgcga actcgccaga agaacagcag tacactgagc aacacccctg
     2641 ctacgccgct cgcgcccact tcgcccaggt cgcgcacgat cgcgtcgtac tcctcgcggc
     2701 tcgtgatcac gtcgcccgtg taatcgacgt gcatgctggg gtcgatgcgc ttcggttcca
     2761 gctttcgcac cactccttcg acgcgacgtc gcagctcgtc gaccgccgcg cgcccgctca
     2821 cgggcgtgcg gatcaagacc gcgatgtacg tcccatcctg gctctcgtaa taacctccgg
     2881 ggtacgcgtc ggcctgggct gctgcttggg ccttgcgttc gctcagcgtc ttttcgatgc
     2941 tctcgcgagt gatcggaggc ggaacctctc cgggctcact ctccagcaac gtgccttggg
     3001 ccttggcgac ctcgtagtcg tagcgctcta cgacttgatc gcgcacccgg cggagctcgt
     3061 cgacctccgc atacagaatc tggttcttct cgaagaaggc ccgcgtctct ttgacgccgt
     3121 agtccacact gccgacccac tccggcccca gcgccagaag ttcgggcacc aacctgtcga
     3181 cgaaagttcg cagtgcttgt cgcttggacc cgtcgtcaat ttgcgccacc aggctcaacg
     3241 tcgaagcgcc cggcaggcgc gccgaaacgc gacgcatctc gatgacgctg tccttgttat
     3301 cgggcaggag ctccgcgaaa tccgacttga atccgaggcg actcgccgcc caacccgcgg
     3361 ggaccaagct caaaaccgcg cagatgataa aacgccaggg cgccgccgct tgcaggcggg
     3421 ccagctcgcg cagcacgcgc ggtagctcgc gttcactcat ggcttctcct tccagcaacg
     3481 agtgcggcgt gctggctgtg ggatctcaac cgaatggcat tctgagcggg tcacgaatcc
     3541 tcgggccggg cgcgggggga gcgagctgct cgaaggcgac gcgcctcgag tctcggcgtc
     3601 tggcttgact agcacgaggc gtgccggaaa cgtgttagtt tccgaagggt cgggcgtcac
     3661 tcgctcggac tcacttggtc gagattcatt cagtgactca gctgtttgct ctggtccgct
     3721 tgtccaatcg acgactcgcg actctcaaag gagtgcgggt ggcgcggctc tatgcgctca
     3781 acatggggtt cggctacgtg ctgttcacgc tcttcctgca cggcgcgagc gtgcgtcggc
     3841 ttggtcaaat cgtgcaggag tccctcagct ttgcgtcttg gttggtgtgc ggcatcgccg
     3901 cgttcgcggt gctccgtgca ccccgaggat tcgacacgag ctgggcggag ctggggcgct
     3961 tgcgaggtta cacgagcgca caccttcaaa aagcttattg gctgtcgctc ggcctgcgcc
     4021 tcgcgctcga cctggtcatc gcggttttcc cggctctgct cgtgatcgcg ctgctcgccg
     4081 atgctccgac tttgggacaa cgcgtcgggt ggccgttgct gggcggcctg tatgcggcga
     4141 gcttgggctt ggccattgcg ggcttggtcg tgctggcgca gcgagccacg agggaccacg
     4201 ccgtatcgct atttctaagc ttgatgatcg ctccagagtt gctcgccatc ttcaccacgc
     4261 tgccgggttt cgggcttccc ggcttgttca gtcgagcatt ggattggatg ttcgtcatcg
     4321 cgccgctgcg gctgggagtc ggatgagcac gctcaagctg tttgcgttgg aggagccaga
     4381 tttcggtccc ttgtcgctcg aactcggctt gggggtgtcc gtaatcgtcg atgagcgagc
     4441 gccgcgcctg gggcggctcg cggaaattct tgccggcgtg cgccgctcga gaggggggcg
     4501 agcgctactc ggcgagcgcg atctgtacac gacgcgtgag gctcgggcga gcacgtggag
     4561 cttactgccc cgggaagagt tcacggctcg agattcggaa gagaccgttg cgcgcgtgct
     4621 cggggatatc gcgcgctgga agggggacca cgagcccatc gagccgcgct tggaccaagc
     4681 ggggctcggc cgctacgcgc cagctcgtgc ggcagagctc gatcccgagc aatgccgggc
     4741 tttgatgctg gctctagctc tcactgctcg cgccccagag gccctgattc tgcacgatcc
     4801 tttggcatgt gtgccttggc tcgaggaagc cttcatcgtg caacgttgcc gggcccaagc
     4861 tcagctctgc ccggtgctcg ttaccacctc cgccctgctg gacgcagctc gcttgggtgg
     4921 cagcgtctcg ctgctttggg gcaagaactg gatcacgccc cgcgagctcg tggctccgac
     4981 cccgagttgg gtcgcagtgt ccgttcgggc gcggccgatc cgagcgctgg ccaacgcgct
     5041 cgctgaacgg gcttggatta cctccttcag agtggacgcg gagctacccg acgggagttc
     5101 ggattcggct ccagacgccg ctggcgtgct cacgcttcag gggcacgact tggcggctct
     5161 gtcggccttg gtcgtaaaca tcgcgctcga acaaggcacg gctctcgagc tgatcgaacc
     5221 gctcgggcgt gtgccgtacg gggggcgttg atgctctctt cttcattccc cacgcccgcg
     5281 tcgctcgtgc acgagggcct gctcggcgcg agagccatcg cgcggcgcgt gccgctgaac
     5341 gcggcgcttc tgggttggtg cgcctgcttc gtctgcgcct ggcttactcg ctctcgagac
     5401 ggcggcgtcg atgatgcgct gaacgtagcg ctcgggctga tcatgccgct caccgcctac
     5461 gttagcttcg aaggcgtgct ggaccgaggg cggctcgaga gcgcggtgcg ggggctctct
     5521 cgtcatggtc tccacggtaa agccttgtgg ctgggcgcgg ccgccgctct gctcggttgg
     5581 ctagcgatga tatcggccag ctttgcggca accgtcgtgt gggtttcgcg cggtgtgtcg
     5641 agcgacgtgc tgctgagcgc ctggataggg gcttggggtg gggccgcgta tgccttgtgg
     5701 ttcagcgcgg cgtccgacgt cggtgcgcga gggcaaggtc gcaaatggct gctgctcgtc
     5761 gacctgacat tcggttccac caatggtttt ctggctttgc cgtggccacg cgcgcacgta
     5821 cgaaacttgc ttgggggaga gtcttggctc ggcttgcagg gcccgtgggc tttcgcgctt
     5881 ttgttcgtga gtggcctgat cgctctcgct atcggattcc gacgggcgcc cgaatagaac
     5941 aaacctgtct cgatcagagc ctgattcggg gctcttcacc tgaggtgcgt tacttgtccg
     6001 atccctattg acaggctagg ctcgcagggc ttgcaggggc tcatttaccg attcgaaacc
     6061 ctagaacagc tggcgcaggc tctcgaagcc agtgcggcgg accgcgatct ccctgttccg
     6121 tgcaggaagg gcgtgaccga aggtgagttc gtgctggtca cgctgagcgc agccgacgaa
     6181 gcgacgagcg tggttgcgcg cgtgcgagaa ggcggtcgcg gcttgcgtct cagcttcgag
     6241 caacgcgact gggagctgct cgaacgtttc gcgcaggggg gcgggcggcc gacgctgccg
     6301 cctccgtgcg gcacggcaaa ccctgaaccg gtgcgggccc ctgcgggcgc caccgcactg
     6361 ctggtcgata cggatgcctc cgtactctcc atcgtgcgcg cgatgctgga ggcgtgtgac
     6421 gtggcgaccc aacaggctca cggcgccgaa gacgcgctca atttgctgcg tcgtcggcat
     6481 ttcgatctag tggtggtgga gcccgcattg gacggcatga gcggcctgga gatgtgtcgt
     6541 cgcttgcgcg ccgatcgcag attggcgggg gtaccgatct tggtgctcgg ttcgcacacc
     6601 actgatcacg atttgcgcga ggcgctctgc tcgggcgcgg acgatttcgt ggcgaagccg
     6661 tttcgcgcac acgagctgcg tgcacgcgcc ctcggcctga tccaaaaggg tcgcgcgttg
     6721 gcggccagcg tgcgcccggc ctgaaatcgg tcccaggccc tgcgctgccg aactcaggcg
     6781 ctgcgacgcc gcttcttctg tggcgcggtg cttatgtctt tggccaggtg tttcaagacg
     6841 cgcgcgggca cggcttggct ctcgaaccac gcgaccagcg cgggctcgta gccgcgggac
     6901 caggtgtcgc tcagccggtc ttgcaggaac tgcgcgtaga gcccgtccaa aattgcgaat
     6961 agccgcagca gtagttccat gcgctcgagg aaggcttgct ccggttcctg ggtgagcaca
     7021 gccgggactt gcgcggtcgc cagcgcgtaa cgaagcgcgt cgagcacgca cgcaaactcg
     7081 ggttctgtgc tctgcatcac cacccgcgca cgcaccggga atttttgcgc tttgaccgcc
     7141 tcgcgcgcct cactgctatc cgacggcgcc gagccacgca ccgtcacacg ctcgttggca
     7201 tcgagcgcgg attgcagggt gatctggcga tccaaccaga cctcgtactc gccgtgctcg
     7261 cccaagctga atggaccttg ggtgagctcc gcgcgcaacc acagccacaa caggaactcc
     7321 gggcccagaa agcgcgtggt ctcgacgcgc gtcgagagcg cgtcctcttc cggatccacc
     7381 ttttcggcgc cgcccgcctt gtctgacgcg gccttcttcg cggctggagg cttcttttcc
     7441 gcgccggctc gcgagccctc gtccgcgccg gtttcactgg ttggttccgc gtctaccggg
     7501 tcggccgggc gcggcttggg gctgggcgag cccgcgacga gcgcggtggc cggctcgggc
     7561 gcggcatccg gtaacccgag ctgcttgcgc ccgttgacgt agctcaccgg catcacccga
     7621 tcgagcgcct tcaggatggt tgccggcagc ttcgcgcgct tggcgaccac gtacgggctg
     7681 tcctcgatca atcgaagctg gaacgtcttc tcgaacagcg cacacagctc ttccccgagc
     7741 cggcgcgagt ggctgaagaa gaacaccacg cccgcgtcca aatcccagca cagatcgaac
     7801 gcgcgcgaga tgggcaccag cttgcgtcgc agcttcacca cgacgcgctt cttcagctct
     7861 ttacgttgct ggcgcgagat cttctccgcc cccgtcttgg tcagcagctt gcgttcctca
     7921 tccgccaact cggcgttgat agcggagctc gggatccgaa acttgtccac ccgcagcccg
     7981 agctgaagca agcggtcgtt gaacagcttg tcgagctcga actccaaatc cagcgggcgc
     8041 tccatgacgc accagccggt agcctcggtg gcttcgtctt cgggcttcaa gggttggaat
     8101 gccctcagcc gaaccgcttc caggtaacgc tttcgcaggt cccgcggggg ctgaccatcc
     8161 acgaagtatc gaaccaaagt aagacttccg cgacgcgcac tcattccaat aaacctcggc
     8221 gagcctcagc cgtttcgacc gcaattagca gcaagcccgc gcatcaattc tctcgaaacg
     8281 cctcggccgg acgcagtcgt gccgcgtgca gggcgggcca caccgtcgcg atcaagcaca
     8341 ccagcaccgc gaagattccg accatcacga agtccatcgc gcgcacctgc acgggcaagc
     8401 gagagatgaa atacaccttg ggatcgagcg gcaacccgta cacgagcagc accttgcaga
     8461 gcagcaagcc gaggcccagc cccgccgcgg tgccaagcat gcccaccagc gtgccttgat
     8521 acacgaacga gcgcagtaag gcgaagtccg tggcgcccat cgccttgagc acggcgatct
     8581 ctttcttctt gtcgagtacc accatgatca gcgtcgcgat cacggtgaag gcggccacga
     8641 tgatgatcag agccagcacg atgctcatca ggatttgttg gatgcggagc gccgtgaaca
     8701 agccgtggtt cagctcctcc cagtccatcg tgcggtagat tccgttgctc agcttcttct
     8761 cgatctgctc ggcgatcgtg cgcgcttcgt cgatatcttt gaccttcatc tcgacgccat
     8821 ccacgctgtc gcccgagtcg taaaaggcct gcgcttcgta caagtcggtg taggcgagct
     8881 tggagtcgta ctggtcgaag ccggcgtcga acaccgcgat cacgcgaaac tgcttggcaa
     8941 cgggcgcccg gatcgaaccc ttggagtaag aaaagccgat cgtgggggag gtgacttgaa
     9001 tgcagtcccc cagggcaacg cccaagttct tcgccaaggt cacaccgacc acgatgccgg
     9061 gcagcgccgc ggtcgagttc ttgttcgaac aaggatcggg atcgagctcc tccggcagca
     9121 cgtcgtcgtc gggcagcacg ctcttgaagc cgccttcggg ctccacgttt ccgatcagtc
     9181 cgttcgcggc gggcgcttct gggggcggct ctacctcggc tctcgcggcc tcaatcgcct
     9241 cggcgggagt cccgctgggc cgctttgggg tcgcgctcga cgcgctctct gggtcggtct
     9301 tggtcttgct gagccgttcg agctcctgaa atagagtctt cttggggccg ttctcgtcgt
     9361 ccgcgctgga cgccgcgcgc ggaacatcat ccaggagtga ttgccggcgc cgctccgcgg
     9421 gcttcgcggc gggtaagcga agccccgcga gcgagccttt cacgatgtct tgcggcaatc
     9481 gcagcacctc ggagactccc accgggtcga cgcccttgag caacacgccc gtcgcggttc
     9541 ggttgccgtg cgtcagcatc atcggcttga tcgtgaacgg cgcgaccccg atcaccccgg
     9601 ggaccttgcg catctggtcc atgatctttc ggtactcgcg aaagtcgctc gagtacttca
     9661 gcaccaacac gtgcgcattg acgcccagca ccttgtcacg gaattgactg cgaaacccgc
     9721 ccgtcacgct gatcaccgtg gatagcgctg ccaccccgag catcacaccc aggattgcga
     9781 agagcgtacc gacggagacg aaggcgcgct tcttcgaccc catgtagcgg agagccagct
     9841 cgagcgggaa acccatcgct gcccgtctag cactgacccg gggcgctaag cagcgctatc
     9901 tgccacggca tagtaacgtg gtcggccggc tgatgtgatc gcattgagca tggctgatca
     9961 atctcacagc tgcagaactc tagagatcgg tcaaagttcc ggagcactac ggttgggagg
    10021 cgctggcggg gctcaagtca gaccctgcac acgcaggccg aagaatcaga ccctgcacac
    10081 gcaggccgaa aagccagacc ccgacgcacg cggaagacag gcgaggcgca gcgccaaaga
    10141 tggaccggga gcgccatgga taagtcgagg tcgcctgcgg agaaacagaa cgccctgcac
    10201 aagcaaggct gaaccagaat gcgggcgatg agcggcgagt ccgggagaac tgaaatgcaa
    10261 ggcggggtga acagagcccg aacaaaggcg gggtgaaaca acagccggtt gaaacagagc
    10321 cccgaataga gctgccggag caagccaaag tcacctcgtc accgccacgc cgaagcgacc
    10381 gccttcagct agccgaatga ctcggccgga ccctcgacgc gagaaagcac aaccgccgct
    10441 cgacgttcca cgagatatcc acagccactc gtctgagcag caacgcgctg agccgctcgc
    10501 gaaacctggc tggcacgaga atgccgcaat gaagatccca cccaatgaaa tgaacacccg
    10561 atcgatcgaa tggaaagggc aaggaggaac aggcgtatag cttggctacc tggctaacta
    10621 gcccggttgt ttggcttagc ggccaacgcg atcaaccccc ttgagcccgc gacaaatcga
    10681 cggtttaagt aacggtccgg atttgccgtc acccggcgct ccgcatgctt aactctccag
    10741 actttctttc tgccccgcag gttactcaca gcttgtgcga gtcctgggga taacttccgt
    10801 gggtttcttt tctcacgttt cgaactagcc gagatttcag atgagcgagc cgcaagacgg
    10861 tagtccttcg gatggcgggc tagtcccggc ggtagggagt gcagcgctct cttccgcgcg
    10921 ggagagcgtg cggcctggag tgatttctag ccggcccccg ccgcgaatca gcagccgccc
    10981 cccggctcct tcgggaggca cacgcctgaa gatcgtgacc gtctcgcccc gtgagtcttt
    11041 cccggaaatg gaagcaccca cgggcgagcg ggaggtacag cgccgcgatc cttatatcgg
    11101 cgccacgatc gacggccgct acaaagtcga ggccgtgatc ggcgagggcg gcatgggcgt
    11161 cgtgtatctc tgctcgcact cgatcatcgg caagaaggtg gcgatgaaag tgctgcgtgc
    11221 cgatttggcg cgcgacacgg aggtgaccga gcgcttcttg aacgaggcca aagccgctag
    11281 cgcgatcggc aatccgcaca tcatcgacat cagcgatttc gggcaattcc ccgacggcgc
    11341 gacgtacttc atcatggagt acctcgtggg cacacagctc tccgaaatgc tcgggcccgg
    11401 ggacgtagta cccgtgccgc ggacgatcag catcggtcgg cagctggctg agggcctggc
    11461 cgccgcgcac gcggcaggca tcgtccaccg cgatctgaag cccgaaaaca tctttttggt
    11521 tacgcgcgga gccgagaagg agttcgtcaa gatcctggat ttcggcatcg cgaaggtgtc
    11581 caccaccagc gaaggcaagt tgacgcgcgc gggggcggtg ttcggcacgc cccactacat
    11641 gtctccggaa caggccgaag gcgcacccgt cgatcaccgc ggagatatct actcgctcgg
    11701 cgtgatcatg tacgaaatgg ccgtaggcca ggtgccgttc gacgcggaga acttcatgtc
    11761 gatcttgcag cagcacgtgc acaagttgcc cgtgccgctg cgctcggtag agccgaagct
    11821 gcaggaggtc cccgtcgagc tcgaagcgat cgtgctcaag tgcctctcga agcggcagga
    11881 agatcgctac caatcgatgc acgagctgct ctccgatctg gaca
//
LOCUS       DASTKU010000011         4644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1339534, whole genome shotgun sequence.
ACCESSION   DASTKU010000011 DASTKU010000000
VERSION     DASTKU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339534"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VFQ61_00355"
     CDS             complement(<1..642)
                     /locus_tag="VFQ61_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9952916.1"
                     /translation="MNPAESSMVGVVPLLVAIAVIVFLLFGMLAMLSRFYRQVDQGRA
                     LVVNTLRADPVVTFTGSMVYPVINRAEVMDLSVKTVEIDRRGKEGLICNDNIRADINV
                     TFFIRVNKTVDDVLKVAQSIGCQRASDPAALSELFTAKFAEALKTVGKHFNFEDLYMK
                     RDEFKDKIIEVIGKDLNGFILDDAAIDYLEQTPIEHLDKDNIMDADGIRKITDL"
     gene            complement(714..1505)
                     /locus_tag="VFQ61_00360"
     CDS             complement(714..1505)
                     /locus_tag="VFQ61_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HET9952917.1"
                     /translation="MAEVLRLSLSFPAVFYTALLGVVLVYWLFVIIGAVDLSQTADGL
                     GTDAGMDGFDADAVVGATKGAVEGAAKGVLEATVSLEGTGGEAGGDGDAGEMTDADTD
                     GPIAAVVSALGLRSVPATVVFSLLITLAWLLTVVSMQLTARHAPEAPHWTAWLALTVA
                     PVLALLPTALLVRPLAPLFAHRKAPSRSDLLGKTCIVRTGTVTPSFGEAMLEDGGAGL
                     IVRVRVDGPRPLKRGDHALLIDWDPAREAFVVEPLEELLGSEQPH"
     gene            complement(1515..1982)
                     /locus_tag="VFQ61_00365"
     CDS             complement(1515..1982)
                     /locus_tag="VFQ61_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HET9952918.1"
                     /translation="MNERALLTLGLMLSAASGTSPEQWLSSVHERLRRAQAASPMNAA
                     LGTVLVGSLLFYAAERGTNPKVQTWYDALVYVSTNLSVGYSDILAKTPLGKALGSALM
                     TYGPALAARVFDPPEASGTVRESASESSLREVADKLDAILSELRAQRAPSSAE"
     gene            2672..3829
                     /locus_tag="VFQ61_00370"
     CDS             2672..3829
                     /locus_tag="VFQ61_00370"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9952919.1"
                     /translation="MMSRLCRLLLLAALSACSSESGSAWPPSSENSPYPEAAAAHLQR
                     TLNEVVRTGVAPGVSVVVDESGYAPWAGSAGVRDLETGQALAPKHRFRAGSMLKVAIA
                     TAVLQQVESGVLSLEQRLPGLLPEEIVARIPHAAEITLRMLLNHTSGIAELTDEEFDR
                     RVAADPTHVWTLEELLDRAFKHAPSFEPGAGWSYSNTNYVLLGEILSRATGKPWRWLL
                     RENVFARAEMFDTQLPEEGNTLCDGCSRGYEFVEGTLIDLTEVDPSMCGAAGGDALVT
                     TPADLAKLLVALIRGALFESPATLERMREFTVAPDPEQAQTGYGLGLARLQVGSTELI
                     GHIGGTAGFQGFMLYRPSTGMTVSGFMNRRGDLAAFVLPGLEAIERGGTTE"
     gene            complement(3849..>4644)
                     /locus_tag="VFQ61_00375"
     CDS             complement(3849..>4644)
                     /locus_tag="VFQ61_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952920.1"
                     /translation="ASDLEINMATETDFTIGLRRELQSNPSLARDLYGSEALPMIFQY
                     DPFNYWIESDAERNLLFTLNRLENVSPRIRYNLHDRGLVRPRAEVDAILADHRLSWSA
                     LSRSALSSSASSSSALGPKSSADTSNANGAPLPLPLLFHWGRQNSAVAFYGCKITPED
                     VQHTILAIPELASRVENFALHPYEDADANKRLELWFELKANVDRLEASEPLTSAVLQG
                     LAHQNQDFRESLRMIPASRRPSLKIFPYGQSPISGQDVRIKRRYIV"
BASE COUNT          908 a         1423 c         1419 g          894 t
ORIGIN      
        1 gaggtccgtg atcttgcgga tgccatccgc atccatgatg ttgtctttgt ccaggtgttc
       61 gatcggggtt tgctcgaggt agtcgatggc ggcgtcatcg aggatgaacc cgttcaagtc
      121 tttgccgatc acctcgatga tcttgtcctt gaactcatcg cgcttcatgt agaggtcttc
      181 gaaattgaag tgtttgccga ccgtcttcag ggcctccgcg aacttggcgg tgaagagctc
      241 cgagagcgcg gctgggtcac tggcgcgttg gcagccgatc gactgggcca ccttcaatac
      301 gtcgtcgacg gtcttgttga cgcggatgaa gaaggtgacg ttgatgtcag cgcggatgtt
      361 gtcgttgcaa atcagacctt ccttgccgcg gcgatcgatc tcgaccgttt tgacggatag
      421 atccatgacc tcggctcgat tgatcaccgg gtaaaccatg gaacccgtga acgtcaccac
      481 cggatcggcg cgcagcgtat tgaccaccag cgcgcgccct tgatcgactt gccggtaaaa
      541 gcgcgaaagc atcgccagca tgccgaacag caagaatacg atgaccgcaa tggccaccaa
      601 cagcgggacg accccgacca tactcgattc cgcaggattc atgttgcctc tagatgactc
      661 tacaattcaa acctgacgcc gaaacgtcgg accgaaaatc tctctcgaac ttctcagtgc
      721 ggctgctcgc tgcccaatag ctcctcgagc ggctccacga cgaaagcctc gcgcgctggg
      781 tcccaatcga tcaacagcgc gtgatctccg cgtttcagcg gtcgaggccc atccacacgc
      841 acccgaacga tgagccccgc accgccatct tctagcatgg cttcgccgaa actaggagtg
      901 accgtcccgg tgcgaacgat acacgtttta ccgagcaagt cggagcgcga tggggccttg
      961 cggtgcgcga acaacggcgc gaggggacga accagaagcg cggtgggcaa gagcgcaagt
     1021 acgggtgcca ccgtcaacgc gagccaagcc gtccagtgcg gcgcctccgg ggcatgccgg
     1081 gccgtgagtt gcatgctcac gacggtgagc aaccacgcca gggtgatgag cagactgaat
     1141 acgacggttg cgggaaccga tctcagtcct aaagcactga ccacggctgc gattggacca
     1201 tcggtatctg catccgtcat ctcgccggca tcaccatcgc cgcccgcctc gccgcccgtt
     1261 ccttcgagcg ataccgttgc ttccagcaca cccttggccg cgccctcgac tgcgcccttg
     1321 gttgcgccca ccaccgcgtc agcatcgaaa ccatccattc cggcgtcggt acccaacccg
     1381 tccgccgtct gtgaaagatc gaccgcaccg atgatcacga acagccaata cacgagcacc
     1441 acacccagca acgctgtgta aaacacagcc gggaaagaca ggctcaatct cagtacttcg
     1501 gccacggtct cgccttactc agcgctggat ggggctcgtt gggcgcgcag ctcgctcaaa
     1561 atcgcatcga gcttgtcggc gacctcgcgt agtgagcttt cagaggcgga ttcgcggacc
     1621 gtgccgctcg cttccggagg atcgaaaact ctggcggcta acgccggacc gtaggtcatc
     1681 agcgccgaac cgagcgcctt tcccagaggg gttttagcta agatatccga atagccgacg
     1741 gacagattgg tcgagacgta gactagcgcg tcgtaccaag tttggacctt gggattggtt
     1801 ccccgctcag ccgcataaaa caacagcgag ccgaccaata ccgtgccgag cgccgcgttc
     1861 atcggcgacg cggcttgggc ccggcgtagc cgttcgtgca ccgaagagag ccactgctct
     1921 ggggaagttc ctgatgctgc gctcagcatc aaccccaaag tcagcaaagc acgttcgttc
     1981 atttcaccac cacaccgcgc agagcatacc tcttcgttcg ctcacgtcga caaaccttta
     2041 cgtacacgat gcgaaggtcc cacgtacatc aagcacctac tcggttgagt tcagggctct
     2101 tgaccaaccc agaattacgg ccggaatcca cgcacctata gtccggacgc ccgcctactc
     2161 agcggcagca gtcgatctga tcgagcgaac gcaattcgcg ctcactgcaa ctcagctttc
     2221 tccgagcggg acgcgcactg cgggttccgc agcggcagct gctcggcgcg ttgccttctc
     2281 cgcccgcggc caccctctca accggaggcc cgattcgttc cgtggacttg gaagaggcca
     2341 tcgcgtcgga gaattcgttc gaattgcggg cagcgcgcac ttgcgccttt cgtgcggagt
     2401 agctgctgtc gacctgcgtg gcatcaggtt gctgcgtcgc gcttccggca atgacggttg
     2461 cgcgggtgcg taggtggcgc tgctcgccgc gttgcccgag gccgcacggg tcacgacccg
     2521 gcatacacaa agagcggatc ccaaccatgg cgacggctcg gtcgggctgc cgccccaatt
     2581 ccttgcgagc gcaatggtag gtgaatcgtt aggtctttga acactaatca gcgctccggg
     2641 tacggattcg cgaacttccc ttcaggagct catgatgtcg cgtctgtgtc gactgctgct
     2701 actggccgcc ctctctgcct gctcgtccga atcgggatcg gcttggccac cgtctagcga
     2761 gaacagtcca tatcctgaag cggcagccgc ccacctgcag agaacgttga acgaggtcgt
     2821 tcgcacggga gtggcgcccg gcgtttcggt cgtggtcgac gagtctgggt acgctccgtg
     2881 ggcgggcagc gccggagtca gagatctcga gaccggtcag gcactcgctc ccaaacaccg
     2941 cttccgcgca gggagcatgc tgaaggtcgc gatcgcgacg gcggtgttgc agcaggtcga
     3001 gagcggcgtg ctgtcgcttg aacagaggct gccggggctc ctgccggaag agatcgtcgc
     3061 gcgcatcccc cacgctgcgg aaattacctt acgcatgctg ctcaaccaca cttccggcat
     3121 cgcggagctg accgacgagg agttcgatcg gagggtcgcg gctgatccaa cccacgtttg
     3181 gacgctcgag gagctgctcg accgcgcatt caagcatgct ccatcattcg agccgggtgc
     3241 gggctggagc tactccaaca ccaactacgt gctactcggg gaaattctga gccgcgcgac
     3301 gggtaaaccg tggcggtggc tgctgcggga gaatgttttt gcgcgcgcag agatgttcga
     3361 tacacagttg ccggaagagg ggaatacgct gtgcgacggg tgctcccgag gctatgaatt
     3421 cgtcgaaggc acactgatcg atcttacgga ggtcgatcct tcgatgtgcg gcgcggcggg
     3481 cggcgatgcg ctggtgacca cacctgccga tctggcaaag ctgctggttg cgctcatccg
     3541 gggagcgctg tttgaatcgc ccgcgaccct cgagcgaatg cgcgagttca cggtcgcccc
     3601 ggatcccgag caggcccaaa cgggttatgg gttgggcctg gctcgcttgc aggtcggaag
     3661 cacggagctc atcggccaca tcggaggaac cgccggcttt cagggcttca tgctgtatcg
     3721 accgagcacc ggcatgaccg tgagcggctt catgaaccgt cgcggtgacc ttgctgcctt
     3781 cgtgctcccg ggtctggaag ccatagagcg tggcggcacc acagaatgag cagaaagagt
     3841 cgtcgccctc aaacgatgta acgacgtttg atgcgaacgt cttgtccgga gatggggctt
     3901 tgcccatatg ggaagatctt cagactggga cgacggctag ctggaatcat ccggagcgac
     3961 tcgcggaagt cctggttttg gtgggccaag ccttgtaaca cagcggaggt gagcggctcg
     4021 ctcgcttcga gtcggtcgac gttggccttc aactcgaacc agagttcgag ccgcttgttc
     4081 gcgtccgcgt cttcataggg gtgcagggcg aagttctcta ccctggacgc gagttcggga
     4141 atggccaaaa tcgtgtgttg aacgtcctcg ggcgtgatct tgcatccgta aaaggcgacc
     4201 gcgctgttct ggcgacccca gtgaaaaagc agcggtaagg ggaggggcgc tccattcgcg
     4261 ttgctggtgt ccgcgctcga cttcgggccc aaggcgctag agctcgacgc actggagctc
     4321 aatgcactcc ggctcaatgc actccagctc aaccggtgat cggcgagaat ggcgtcgacc
     4381 tccgcgcgag ggcgaaccaa gccccgatcg tgcaagttgt agcggatgcg tggagagacg
     4441 ttctcgaggc ggttgagcgt aaacaggagg ttgcgctccg cgtcgctctc gatccagtag
     4501 ttgaaagggt cgtactgaaa gatcatgggc agcgcctcgg agccgtaaag atcgcgcgcc
     4561 aagctcgggt tcgactgcag ctctcgccgt aacccgatcg taaaatcggt ctcggtggcc
     4621 atgttgatct cgaggtcaga ggcg
//
LOCUS       DASTKU010000012         4608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_1875,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000012 DASTKU010000000
VERSION     DASTKU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFQ61_00380"
     CDS             complement(<1..206)
                     /locus_tag="VFQ61_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952921.1"
                     /translation="MRVRRSAIASTLSLLLATQAVLAQTNGNGVGGAAGASVTPTTPG
                     GSTPAATGGAAKSPANTSTAAGASS"
     gene            complement(206..2626)
                     /locus_tag="VFQ61_00385"
     CDS             complement(206..2626)
                     /locus_tag="VFQ61_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9952922.1"
                     /translation="MTRPWLLRGSWCVALALASVAKPGWALDAAEAQRRALTTISQVE
                     TEVLRGGSAPRELFAPPSPAERIAAGDMLLRTKDLDRAVNELSKVLELYRQGKVPEAA
                     YADASFLIGEAYFQSRQYLAARRHYREVLDQAQRPTFESYVGRAVSRLVDVALRTGDL
                     ESLDYVFARLNRLPATDRSGSLAYARAKALFAHKELAAAKQAISVVPAGSDYAMQSQY
                     LLGVIYVREAAPPPSVNPPSPAPGAAPVAPPTAAAAPSTPPQRYVAAVEQFRSVTRMP
                     ARNAAQKHVVDLAWMAIGRLFHEMDAYLDAAEAYSHVDRASPEFSSMLYELAWVYVRL
                     GDYMRAQRALEVLAIMDPENIEAADGSLLRADLMLRSGQFEKALALYKSVQGKFDPIR
                     DQVDRFLQSTNDPAVYYDRLTADPSIPSSDDRLPGVVVEWAREQAEDEHVFGMIEDVN
                     RSRDLIRSSRKLASKLNAVLGGPTRIRAMPELRAKMQRSLSLLNRASRSRVTLSEGLD
                     DVSPQSSGELTSVRQQRRALMRRMGYLPQTDVEFSKREESGERQWDKVSQQLQGLMLE
                     ADRLNAVVNGLRRVLREADQHGVTSDPASRERFQVEIEQNERDLVTYRRRIEEYKNAM
                     DLGRAQIGFGDQRYSEDEDVRRRFRELLAREVALCASGQGGEDATAYARSIQPLLLRA
                     DVLESRLEGEVRSYDEQAGAQAATLLKKVESEVGLLETSSQNLDTLDQQARLLVGEVA
                     MRNFALVRDRLKNVVLRADVGIVQEAWEVREEQRVRVRNLQRERSREESSLNDELREV
                     IDDAAEDQ"
     gene            complement(2623..3609)
                     /locus_tag="VFQ61_00390"
     CDS             complement(2623..3609)
                     /locus_tag="VFQ61_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952923.1"
                     /translation="MIRVDRACMVAALALSVSWVSRSHAQSSGQPVQSNPSTQSQTAA
                     GTPSTATGASTKPADGNKTTTTGASPGAAASPGVTGGTSSGATAPSTGAAPTTAAPTT
                     TSTTASGASGAASGGAGLDGSTYVVRLRDLEQRIDELKEQIRRSHTRLSLLSDTILSG
                     GVGGSRAEVVFRNDLSNAFRLTGAVVVLDGVVQYNKQDDSDNGLLGSQKEIPIFSGSI
                     PPGDHTVQVVVRLQGHGYGVFSYLRSYKFDLKASHQFTITEGKTIGLDIIAWEKGDVT
                     TPLDQRPAVRFGQTLRSGLADPAAAGGKPAAPPPAAPSTSISGGASLSTGTK"
     gene            3984..4604
                     /locus_tag="VFQ61_00395"
     CDS             3984..4604
                     /locus_tag="VFQ61_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9952924.1"
                     /translation="MPAVQGDATDEMLMVRYQRGDAEAFATLARRHTRPVYNFVLRKL
                     RDVAAAEDVAQEVFLRVVQNAGEFKHEARFSTWLYSIARNSCVDHQRRMSHRRHASLD
                     RPAPGKSEASEPLGAHLPDLRPDGNAERSYVSSKVQASILNALDALPDDQREVFLLRE
                     VANLPFKEIAVVTDTPENTVKSRMRYALDRLREALSDFEEYARALR"
BASE COUNT          817 a         1551 c         1401 g          839 t
ORIGIN      
        1 gagcttgccc ccgccgcggt cgaggtattc gcgggcgact tcgccgcgcc gccggttgcc
       61 gcgggggttg acccgcccgg cgtcgtcggc gtgacgctgg cgccggccgc tccaccgacc
      121 ccgttcccgt tggtttgggc cagaacggcc tgggtggcca gcaagagact aagcgtgctg
      181 gcgatggccg aacgtctgac gcgcatcact ggtcctccgc cgcgtcgtcg atcacttcac
      241 gcagctcatc gttcaaactg ctctcctcac gagagcgttc gcgttgcagg tttctcacac
      301 ggactcgctg ttcttcgcgc acttcccagg cctcttgcac gatgccgacg tcggcacgca
      361 gcaccacgtt cttcagccgg tcgcgaacca gcgcgaaatt gcgcatcgcc acctcgccca
      421 cgagcaggcg agcctgctga tcgagcgtgt ccaagttctg cgatgacgtc tcgagcagac
      481 ccacctcgct ctccaccttc ttcaacaagg ttgccgcctg cgcgcccgcc tgttcgtcgt
      541 acgagcgcac ttcaccttcg agccgggact cgagcacgtc ggcgcgcagc aacagcggtt
      601 gaatcgatcg tgcatacgcg gtcgcgtctt cgccgccctg accgctcgcg cacaaagcca
      661 cctcgcgtgc gagcagctca cggaagcggc gccgcacgtc ctcgtcctcg ctgtagcgct
      721 gatcgccgaa gccgatctgg gcccggccca agtccatcgc gttcttgtat tcttcgatgc
      781 ggcggcggta ggtcaccaag tcgcgttcgt tctgctcgat ttcgacctgg aagcgctcgc
      841 ggctagcagg atcggaggtg acaccgtgtt gatccgcctc acgcagcaca cgtcgcaacc
      901 cgttcaccac cgcgttcagt cgatcggcct ccagcatcaa cccctgcagt tgttgggaaa
      961 ccttgtccca ttgacgctcg ccgctctctt cgcgtttcga gaactccacg tccgtctgag
     1021 gcaggtaacc catgcgacgc atcaatgcgc ggcgctgttg ccgaacgctc gtcagctctc
     1081 ccgagctctg cggggacacg tcgtccagtc cttcggacaa cgtgacgcgc gagcgtgacg
     1141 cacgattcaa gagcgagagc gagcgctgca tcttggcgcg cagctccggc atggcccgga
     1201 ttctcgtcgg gccgccgagt actgcattga gcttggaggc cagcttgcga gacgagcgga
     1261 tcaagtctcg ggatcgattc acgtcttcga tcatcccgaa cacgtgttcg tcttcggcct
     1321 gctcgcgcgc ccactccacc accacgcccg gtagccggtc gtcgctgctg gggatcgacg
     1381 gatccgcagt cagtctatcg tagtaaaccg ccggatcgtt cgtcgattgg aggaagcgat
     1441 ctacctgatc gcggattgga tcgaacttgc cctgtacaga cttgtagagc gcgagcgctt
     1501 tctcgaattg gcccgatcgc agcatcaagt ccgcgcgcaa caaagagccg tccgcggcct
     1561 cgatgttttc gggatccatg atcgcgagca cttcgagcgc gcgctgcgcc cgcatgtagt
     1621 cgccgagccg cacgtaaacc caggccaact cgtaaagcat cgacgagaac tcgggcgacg
     1681 ctcgatcgac gtggctatag gcctccgcgg cgtcgaggta agcgtccatc tcgtggaaca
     1741 agcggccgat ggccatccag gccaggtcca cgacgtgctt ctgcgctgcg ttgcgcgccg
     1801 gcatgcgcgt cacgctgcgg aactgctcga ccgccgccac gtagcgctgt gggggcgtcg
     1861 acggagctgc ggcagccgtg ggcggcgcga ccggggccgc tcccggtgca gggctcggcg
     1921 ggttcaccga cggcggcggg gccgcctcgc gcacgtaaat cacgcccagc aagtactggc
     1981 tctgcatcgc gtaatccgag ccggcgggga ccacgctgat agcttgcttg gcggccgcta
     2041 actccttgtg cgcgaacaac gccttggccc gcgcataggc gagcgatccg gagcggtcag
     2101 tcgcgggcag acggttcaac ctcgcgaaca cgtagtccag cgactcgaga tcacccgttc
     2161 gcaacgcgac gtcgaccaat cgactcacgg cgcggcccac gtagctctcg aaggtcggcc
     2221 gttgcgcttg atccagcacc tctcggtagt gacggcgcgc ggccaagtac tgccgagact
     2281 ggaagtacgc ctcgccgatc aaaaacgagg catccgcata cgccgcctcg ggcaccttcc
     2341 cttgtcgata gagctccagc accttcgaca gctcgtttac ggcacgatct agatctttgg
     2401 tgcgaagcag catgtctccc gcggcgattc gctcagccgg gctcggcggg gcgaacaatt
     2461 cacgcggcgc gcttccacca cgcagcacct cggtctccac ttgggagatc gtggtcagcg
     2521 cgcgcctttg cgcttcggcg gcgtccagcg cccagccggg cttggcaacg ctcgccagcg
     2581 cgagcgcgac acaccatgag ccacgcagca accaaggtcg cgtcattttg tgcctgtact
     2641 gagggatgct ccgccagaga tagacgtaga cggcgcggcc ggcgggggcg ccgctggttt
     2701 accgcccgcg gcggccggat cggccaaacc cgaacgcagc gtttgcccaa aacgcaccgc
     2761 tgggcgctga tccaacggtg tggtcacgtc ccccttctcc caggcgatga tgtccagccc
     2821 aatggtcttg ccctcggtga tggtgaactg atggctagcc ttcaggtcga acttgtagct
     2881 cctcagatac gagaagactc cgtagccgtg cccctgaagc cgcaccacca cttggaccgt
     2941 atgatcgccc ggcgggatgg agccgctgaa aatcgggatc tctttctgtg agcccaacaa
     3001 gccattatcc gagtcgtctt gcttgttgta ctggaccacg ccgtccagca cgaccacggc
     3061 tccggtgagc cggaaagcgt tgctcagatc gttgcgaaac acgacctccg cgcgcgagcc
     3121 gccaacgcct ccggaaagga tcgtgtcgct cagtaggctg agccgggtat gactgcgacg
     3181 aatctgctcc ttcaactcgt cgatgcgctg ctccaagtcc cgcagcctca cgacgtaagt
     3241 cgagccgtcc agacccgcgc cgccactagc cgcaccggaa gctcccgaag cagtcgttga
     3301 ggtagtggtg ggcgcagccg tcgtgggcgc ggcacctgtg gacggagcag tggctccgga
     3361 gctcgtgccg cccgtcacgc cggggctcgc agccgccccg gggctcgcgc cggtcgtagt
     3421 ggttttgttt ccgtctgcag gcttggtcga agcgccggta gcggtcgacg gtgttcctgc
     3481 ggcggtttga gattgagtcg acggattcga ctgaacaggc tggccagagg attgggcgtg
     3541 cgatcgtgac acccaagaaa cgctcaaggc caatgcggca accatgcatg ctctatcgac
     3601 gcgaatcatg accctctccg tacgacagcc ttaagtcccg acttggggtc gggcgaatcc
     3661 caacaccgca gcgaacgcac gctacggctc cttgcgtgag gccgcatctt ctcctcaagg
     3721 tggtgctcac gccaagcagg aaagttgcca cgagaccagc cggtttcaca gaccagccaa
     3781 ccgacctccc tgcctactac ccgacctgca acgaaacgta ggtatcttgc gggcttcggt
     3841 ggcacggcgt agcgtgaaaa ccccgcgcgg accgaggaga tctctctccc tcgtcgaacg
     3901 taatccagtt tcgacagccc aagcacgaac ggtcgtgcca agcacccttt actgcctccc
     3961 cgccgcggaa ctctgatcgg aatatgccgg ccgtgcaggg cgatgcaacg gacgaaatgt
     4021 tgatggtccg ctaccaacgc ggcgacgcgg aagcgttcgc cacgcttgcc cgtcggcaca
     4081 ctcgacccgt ctacaatttt gtcttgagga agctgcgaga cgtcgcggcc gcagaagatg
     4141 tggcgcagga ggttttcctg cgcgtcgtcc agaacgcagg cgagttcaag cacgaggcga
     4201 ggttctcgac ctggctctac agcatcgcgc gcaattcctg tgtcgaccac cagcggcgga
     4261 tgtcccatcg gcgccacgcc tccctggatc ggccagctcc cggaaaatct gaagcctcag
     4321 aacctctggg cgctcatctt cccgacttgc gtcccgatgg gaacgccgag cggagctatg
     4381 tttcgtccaa ggttcaggcc tcgatcctga acgcactgga cgctctaccc gacgatcagc
     4441 gggaggtttt cctgctgcga gaggtggcaa acctgccctt caaggagatc gcggtcgtga
     4501 cggatacgcc tgaaaacacg gtcaagagcc gaatgcgtta cgcgctcgat cgtttgcgcg
     4561 aggctctgag cgacttcgag gagtacgccc gagcgctacg ttgacgca
//
LOCUS       DASTKU010000013        11886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1785492, whole genome shotgun sequence.
ACCESSION   DASTKU010000013 DASTKU010000000
VERSION     DASTKU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11886
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785492"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFQ61_00400"
     CDS             complement(<1..297)
                     /locus_tag="VFQ61_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952925.1"
                     /translation="MTPEGSAAGGGTADAIAATAFEAGGATCAAATWAAATWAAATWP
                     AATWAAATWAAAMARAGAGAETGTTGARVVAWLATGAASAPGVTGQGGVLGAVPT"
     gene            complement(334..471)
                     /locus_tag="VFQ61_00405"
     CDS             complement(334..471)
                     /locus_tag="VFQ61_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952926.1"
                     /translation="MAASPLAASPTGGPALIRIAGTGLPDAGLPDEPASVAELGGEAV
                     G"
     gene            611..898
                     /locus_tag="VFQ61_00410"
     CDS             611..898
                     /locus_tag="VFQ61_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952927.1"
                     /translation="MSHGPAAAAPNSVVPSGSNPHDESGIVPRSSPPLRKSSNSQSAS
                     KSPNTGRPASLYAAAPAKAAQSIFGEGVMSEQSLDEVILNYLADDLDTPGQ"
     gene            complement(964..2403)
                     /locus_tag="VFQ61_00415"
     CDS             complement(964..2403)
                     /locus_tag="VFQ61_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952928.1"
                     /translation="MFATGRTRARIEKRKTGANQVGIAANARANERAKRVGGAEQNPI
                     VLSNDGAGQTGWASAHARLGVRAGPIMVGSCCDAACSATTGSKAPLSAASRAFAKGNA
                     TRTRAGEARWRGALWDRAPWIFRAAWLIGIAASIWARPARADAPAEHVAAARALGVRG
                     IKLADQGKCEEAIESLERAEALYHAPTILGRLGECQVLVGKIVLGTENLYRVTRESLP
                     ADAPNAYRLAQTRARNVLQQSLPRIGVLVVRVDPTAAPPSVDVDGVSMPQALLGQDRP
                     TDPGTHWVSVSAAGYEAQRLRVELKEGARQELNVKLVPVPQASGISSEPLLPLPEPPT
                     GAADSPVRESAASERAEAPDRTPAYVCFGLGAAGLVTGAVAGVLALTQRGDLACTNKD
                     CPPDQHADLDRARRFATISTIGFGVAMAGAAAGGALLWFERGGHANKPASARPPQGAD
                     SRRASPSVTRLRIQPWVGWAAAGLNGEFQ"
     gene            2595..3662
                     /locus_tag="VFQ61_00420"
     CDS             2595..3662
                     /locus_tag="VFQ61_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952929.1"
                     /translation="MARKHAYAWFVVGTAWALTHCATVESPPPTEDAGRGGHNTAGAT
                     TTGGAGGATAGASSGTATGGFTGSMSSGGTTNAGGSANSGGALSGGAVGSGGVVANGG
                     SRASGGSPSNGGAAFGGAAFGGAAFGGAVSGGASAQGGKSSGGSTASSGGAATGGRSS
                     TGGAASGGRASSGGATASGGRSSGGATSSGGVAAGGSSGGTGGAPGGSTCKIDGDCAS
                     QQCIASKCAPEHCGDGSMSTSETDVDCGGSCEPCALGKRCSAGADCASNSCGDTGTCS
                     APTTGSCASGWETDPCASTCLNQTQGNLLKCKDVLDCYVAHDCGPGECDGNDQVCGAN
                     KLQIDTAPYPSAATVYACMCK"
     gene            complement(3676..4305)
                     /locus_tag="VFQ61_00425"
     CDS             complement(3676..4305)
                     /locus_tag="VFQ61_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952930.1"
                     /translation="MQFTKSQRVRLWAIGAVSVACSCGACTEGAPQHPSLRESGGFDG
                     PTPALEKQSPSPDRPMFEHPRAVSSGTHFPNRAEVESCGAIVQRVTRRAPAFASLVRN
                     ESAALVFKDEEATGADRMMAPALKARLEGLATLLAREWPGLRLRVTEAWDEDGEHGEN
                     SLHYEGRAADVTASDRDAQRLPRLGGLALEAGFDWVSNEGSHVHVSVRR"
     gene            4510..4581
                     /locus_tag="VFQ61_00430"
     tRNA            4510..4581
                     /locus_tag="VFQ61_00430"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4542..4544,aa:Cys,seq:gca)
     gene            5138..6886
                     /locus_tag="VFQ61_00435"
     CDS             5138..6886
                     /locus_tag="VFQ61_00435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9952931.1"
                     /translation="MGETDPPSRRSKPRLQPGLVLANKYRLERELGRGGMGTVWAAVH
                     QGLGQLVAIKLILAEHAQSQEARERFDTEAKAAARLRSRHVVQVFDNGETPEGMPFIV
                     MEYLEGETLEARMQREGTLSLADAVRVTGQVARALVRAHAQGIVHRDLKPANIFLSKN
                     DDDEPGWTAKVLDFGVAKLSEERDRLTTQPGTVLGTPLFMSAEQVRGASSVDHRADLY
                     SLGMCFYHMLTGQYAFFGETFNDVLVAICTEPLPSLRTAAPQVPAVYEAFYLRCCARD
                     PRGRYQSARELLEALERTANEALAHSAGPHALGAQALKASLDERLGASDGAGLSRTQD
                     EEAPPRSLNRDALFGADESGDYAQPAAGPFSPSPVTLRAATTAAEALTERAPSFDLRE
                     AVAGPFATPPPAPARETELRHSDGGASITVRRVRGTPVRVGVWGLGVVALVAASFVGI
                     KLWRNRDALPRAPVAASLHEVRANTADGVPSSTEPTPHIELVPEESAAHDLTNAQRTL
                     RAEIAQPKAALATPTASSTSTSSPPLPARGSHKPNSSEPPPSNDRAAIPAATPAMNNG
                     SAAAPKPPAPEVDIGF"
     gene            7268..9178
                     /locus_tag="VFQ61_00440"
     CDS             7268..9178
                     /locus_tag="VFQ61_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9952932.1"
                     /translation="MTEHKASPLRVGTLLLGRYEVRACLGRGGHAHVYEAYDAFVDRA
                     VAIKVIGPSESGSQELLRRAQSEARVLARLQHPNIVRMYEAGMTDEGFVYLVMEKLEG
                     RTLLDVSRDPGRLRPREALEIAVQIADALLVAHVQGVLHRDLKPANVFVTADNRIKVL
                     DFGVAKIKDYGAHSTRRDVIQGTMIYMAPEQLQGLTLTPRSDIFALGTMLYELLYRHP
                     VLLGECVPSFAEVGWLQIATLPPMLDTLDPSIPRYVARFVQRCLLKRPEDRFESMLQV
                     AEAARTALERMQHEGKPELLSLRELSLPTGLAIEQQATLHASQKLTLRPYLPTAEPEL
                     EKASNARLRPGLEIHGEHEPRAGARNGPGRTLRILNGPLAPPQASFVHPVATQSTRTA
                     SPRWQAPRAVEAAPVDPNALGSVTETGLVTKARRALGPQKGVRKSVYLFVLVGVAFFS
                     YWQAAGRDQGTRPPLDEVLEKSVVTKRVLAQNLEGQPSSRPASAVTVSAAMAAESAPP
                     AEPSAEQEAQPSASPRAKPSQVSERAEALPDPAAAAKVPRVVTKSARSTRDRAATLFD
                     LPFALPDDEPASSLEAWANSSPPKPASANKPSRAAARSAGLIADPVDEVYGPSTVSPA
                     QPSRGAVQRGAP"
     gene            9175..10347
                     /locus_tag="VFQ61_00445"
     CDS             9175..10347
                     /locus_tag="VFQ61_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952933.1"
                     /translation="MTDNRRYPPPLPAEPSPEFSPVEPQRVPLGMGARAVRPAPKKPH
                     GFVPPASNTAPASPAAKNQGNSSPSANSTAVPAGPAPTGAGVPNASATTRGPGLLSLQ
                     RSQEVALPAGIRQKWLQFQAPRVNPGEFQDTVQPTTAGGATGASTAQGAAGVTAPQTP
                     MAWPTPAASANAAAAHAAKSAAASQKSGSAKAPGKADGKNPARSSAQVRLSPWLVSGL
                     FLIAAGGGWFGIRSFVGRLGSSSARLETATKSSKPAAGVVSGVAIAAAKPGEGNPGEG
                     NPGEGNPSEAGEPATAPTPEATRPGADGVNAEVAVAPAQRNAAKPSAQATKPEVRGAR
                     AEGSATADSRVAESATEAPVTSPPVQSREPHRSPARNSKATKRSSVLDTPIIDPDG"
     gene            10460..11674
                     /locus_tag="VFQ61_00450"
     CDS             10460..11674
                     /locus_tag="VFQ61_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952934.1"
                     /translation="MFHVVALCGAFTGLVTATHSARAQPGAAAQPGAAAQPNPSASPS
                     GPKATSATSAAGTASPGGANSGTPGSGVTGSNEYAQFRKAHDAMKAENWQEARRILLG
                     LWDKSNSYDVGALLGQVEFKLGHYGLATRRMAFALAHIPPKEKPETRERLQAAFKEIR
                     SRAGALRVAVASPNLRVFVDNENVGSTPFDSEVYVDPGTHLVELKDATDRAVASNRNA
                     SVAAGQVAVVDLPITARDAGGTGVLSNPGEPTPISGAESSNGSGVESNSRLPPVGPER
                     HKSLVPVYVAGGVAAAGLAVAIGFGVASRSAKNKAEDHQALLGPSGCSGGGSPACDEL
                     RDERDAWRRDARIANIGWAAFGVGVAAAGVWLLLPGPRHETGAQASQGLALRADVQPG
                     PQGARFSLGGQF"
BASE COUNT         2140 a         3984 c         3858 g         1904 t
ORIGIN      
        1 cgtgggcacg gctccgagca cgccgccctg tccggttact ccgggggcgg aagcagctcc
       61 ggtcgccagc caggccacca cacgggcgcc cgttgttccg gtttcagctc ctgctcccgc
      121 tctggccatt gcggcggccc aagttgcagc ggcccaggtt gcggcgggcc aagttgcagc
      181 ggcccaggtt gcagcggccc aggttgcggc ggcgcacgtc gctccgccgg cctcgaacgc
      241 ggtcgcggcg atcgcgtccg cagtgccgcc tccggccgcg ctgccttccg gcgtcatggc
      301 gtcgaggctt ccgccggcga ctccgtcgca ctctcaaccc acggcttcgc ctccgagctc
      361 ggccacggaa gcaggctcgt ccggcaaccc cgcgtcgggc agtcccgtac cggcaattcg
      421 gatcagcgcc ggcccacctg ttgggctggc cgcgagcggg ctagctgcca ccagccatcc
      481 cgcgccggcg gctcacggtg ccaattccgt tcagggcgtt ggtcgcgcgc ccgtcagcca
      541 tgcccccgtg agccatgccc ctccaaccag ttacggcccg ccgagtcaca gctctgttgg
      601 tcacggccct gtgagtcacg gccctgcggc ggccgcgccc aattcagttg tgccttcggg
      661 ctcgaatccg cacgacgaat ccggcatcgt gccccgctct tcgcctccgt tgcgcaagtc
      721 ctcgaactcg cagagcgcct ccaagtcgcc gaacacaggc cggcccgcat cgctgtacgc
      781 cgccgcgcca gccaaagccg cgcagtcgat ctttggagag ggcgtgatga gtgagcagtc
      841 gctggacgag gtcatcctca actatctggc ggatgatcta gacacgcccg ggcagtaatc
      901 ccctcgccgg gcggtaatcc cctcgcgggg cggtggccgg aagcagcagc cgtcgaacga
      961 cggttattga aattcgccgt tcaggcccgc agctgcccac cctacccagg gttggattcg
     1021 caagcgagtg acgctcggcg aagcccgccg tgaatcagct ccctgcggcg ggcgagccga
     1081 agcgggcttg ttggcgtgac cgccgcgttc gaaccagagc agagctcccc ccgcggccgc
     1141 tcccgccatc gccaccccga agccgatggt cgagatcgtc gcaaacctac gcgcgcgatc
     1201 gagatcggcg tgctgatcgg gtgggcaatc cttgttcgta caggccagat ctccgcgttg
     1261 agtcagtgcg agaacacccg cgaccgcgcc ggtcaccaag cccgcagctc caagcccgaa
     1321 gcacacgtac gcgggcgtgc ggtctggcgc ttcggcgcgc tcgctcgctg cggattctcg
     1381 caccgggctg tcggcggccc ctgtgggtgg ctccgggagt ggcaacaatg gttccgagga
     1441 aatgccgctc gcttgcggca ctggaactag tttcacgttc agctcctggc gcgcgccctc
     1501 tttgagctcc acccggaggc gctgcgcctc gtaacccgct gccgaaacac tcacccaatg
     1561 cgtcccggga tcggtaggtc ggtcttgacc gagcagggcc tgcggcatcg agactccatc
     1621 cacgtccacg ctgggcggag ctgcggtggg gtcgacccgc acgaccaaca cgccaatccg
     1681 aggtagcgat tgctgcaaca cattacgtgc tctggtctgc gccaaacgat atgcgttggg
     1741 cgcatccgcc ggcaaagact cgcgcgtgac gcgatacaga ttctccgtgc cgagcacgat
     1801 cttacccacc aacacctgac attcgccgag ccgccccagg atcgtgggcg cgtgatacag
     1861 cgcctcggct cgttccaaac tctcgatcgc ttcctcacac ttgccttgat ccgcgagctt
     1921 gatccctcgc actccgaggg ctctcgcggc ggcaacgtgt tcggcggggg catccgcgcg
     1981 ggcgggccgg gcccaaatcg aagccgcgat cccgattaac caggccgctc gaaagatcca
     2041 tggagctcgg tcccatagag caccacgcca tcgcgcctcg cccgctcgcg ttcgagtggc
     2101 attccccttt gcaaaggcgc gggaggcagc ggagagcggt gccttggagc cagtcgtcgc
     2161 cgagcaagct gcgtcgcagc aggaaccaac catgatcggt cctgctctta ccccgagccg
     2221 agcgtgggcg gaagcccagc ccgtctgccc cgcaccgtca ttcgataaaa caatcggatt
     2281 ttgctccgca ccacccaccc gttttgcgcg ctcgttcgca cgcgcgttcg cggcaatccc
     2341 aacttggttg gcgccagtct tgcgtttttc gatacgcgct cgtgtgcgac ccgttgcgaa
     2401 cacgactgct gctcacgcgt tcgcggcgca cgcacagctt gtctcgcgag cggtcaggcc
     2461 tgctcgccct gcgcgcgtgt ccggtttagg cgcctgatta actacccgtc tgggtgtgtg
     2521 tagatatcgc aaaacctaac gaagaattca cggactcgag ttgcagatgc gcaacaaatc
     2581 aaaaagactc ttgggtggct cgaaagcacg catacgcttg gtttgtagtc ggtacggctt
     2641 gggcactcac acactgcgcg acggtcgaat ccccaccccc tacggaagac gcgggcagag
     2701 gcggtcataa caccgccggc gccacgacga ccggaggcgc gggcggcgcg accgcgggtg
     2761 catcctccgg aaccgccacg ggtggattca ccgggagcat gagcagcgga ggcaccacta
     2821 acgcgggcgg aagcgcaaac agcggcggag ccctcagcgg cggagccgtg ggcagcggcg
     2881 gagtggtcgc caacggggga agccgcgcga gcggtggctc gccgtccaac ggtggcgcag
     2941 ccttcggcgg cgcagccttc ggtggtgcag cgttcggtgg cgcagtttcg ggcggtgcgt
     3001 cagctcaagg tggcaagagc agcggaggca gcaccgcatc gagcggcggt gcggcgacgg
     3061 gcgggcgctc gtccaccggc ggcgcggcct cgggcggacg cgcgtcttcc ggcggtgcca
     3121 ctgcatcggg tgggcgatct tccggcggtg ctaccagtag cggcggcgtc gccgccggcg
     3181 gaagcagtgg tggcaccggc ggtgctcctg gtggaagcac atgtaaaatc gatggcgact
     3241 gcgccagtca gcagtgcatc gcgagcaagt gtgcgcccga acattgcggc gatggctcga
     3301 tgagcacgag cgagaccgac gtggattgcg gcgggagctg tgaaccgtgc gcgctcggca
     3361 agcgttgcag cgcgggtgcg gactgcgcga gcaacagctg tggagataca gggacctgca
     3421 gcgccccgac gaccggtagc tgcgcgtcgg gttgggagac cgatccgtgc gccagcacgt
     3481 gcctcaacca aactcagggc aacttactga agtgcaaaga cgtgctcgac tgctacgtgg
     3541 cccatgactg cggccccggc gagtgcgacg gcaacgacca agtctgcggt gccaacaaac
     3601 tgcaaataga cacggcgccg tacccctccg cagcgaccgt ctatgcgtgc atgtgcaagt
     3661 agcgacgttc gaacttcagc gtcgcaccga aacatgcacg tggctgccct cgttggacac
     3721 ccagtcgaag cccgcctcga gcgccaaccc gcccaagcgc ggcagtcgtt gcgcgtcccg
     3781 atcggaagct gtgacatctg ccgcgcggcc ttcgtaatgc agactgttct cgccgtgctc
     3841 cccatcctcg tcccaggcct cggtcacccg taaacggagc cctggccatt ccctcgccag
     3901 cagcgtcgcg agcccctcga gcctcgcctt cagcgcgggc gccatcatgc gatcggctcc
     3961 cgtcgcctct tcgtccttga agacgagtgc cgcagattcg ttgcgcacca aactcgcgaa
     4021 ggccggggcg cgtcgggtca cccgttggac aatcgccccg cacgactcca cctcggcgcg
     4081 gttcggaaag tgcgtcccgc tcgacactgc gcgcggatgc tcgaacatcg gccggtccgg
     4141 cgaaggactc tgcttttcca acgccggggt ggggccatcg aaaccgccgc tctcgcgtaa
     4201 cgacggatgt tgtggcgccc cctccgtaca cgccccgcag ctgcaagcca ccgaaacggc
     4261 gccgattgcc cagagtcgaa ctcgctgtga cttcgtgaac tgcactctct aaaccggttc
     4321 ttccgccttc tagcaaggct ctctccgcca ggcgagccgc cccctccccc accctgtctg
     4381 cgccctcgag ttgcaccaga gggagacctg tttcagcacc tggcccgcaa cccaaccaac
     4441 gcggagcaag tttggctagc aaggtgcgaa gcagcatgtt agaagccgcg agccatcagc
     4501 aacgagaacg gcgcggtagc caagtggtta ggcaacggtt tgcaaaaccg tcacacgtgg
     4561 gttcgaatcc catccgcgcc tccgaaagcc gattctcgtg tgtttctgct gcccgcgagc
     4621 tcgcttactg aagcgtgctc gcgaacgagc ccggggagca cagtgacggc agagcagcga
     4681 gggcaaggcg gcagggctca gcggcaaggg cacagcagcg ggggcacagc gaacttgggc
     4741 cgcgacgcag ccagcaggga tggcgtgatc gccaacaccc gaggcgtgac gtaacgcccg
     4801 cctccggcct tcgagcgcct cacccgaacg cctctcactc ctcaggcttt tgcgggccgc
     4861 ccgagggaga cgggcgcgag atccgtcagg tctcataggc ggcgcaggca gcgttgcgct
     4921 cttccgctcg gcgcaccacg ccaacggcac ccaataaccc ggcgcccctt cagcgcgcgg
     4981 catcttcacc ccacgtcccc gtaacgctct cgagtgcgtt tccttctctc ggagcatgac
     5041 tatggcgctc actgcctggg gaggcgtaag ctgtgacact gcaccgctac gaccacagaa
     5101 gaagcccacg gaagaagcga ggagaagacc gaaggagatg ggagagaccg accctccgag
     5161 ccgccgatcg aagccgcggc tacagcctgg cttggtcttg gcgaacaagt accgactcga
     5221 gcgggagctc gggcgcggcg gcatgggaac ggtgtgggcg gcggttcatc agggcctagg
     5281 gcagctggtt gcgatcaagc tcatcttggc tgagcacgcc cagtctcaag aagcgcgcga
     5341 acgcttcgac acggaggcca aagccgcagc acgactgcgc tcacggcacg tcgtccaagt
     5401 cttcgataac ggtgagacgc cggagggcat gcccttcatc gtcatggagt acttggaggg
     5461 ggagacgctg gaagcgcgga tgcaacgcga gggcacgctg tcgctggcgg acgccgttcg
     5521 cgtcacgggg caggtcgccc gcgccctggt ccgcgcgcac gcccagggca tcgtgcaccg
     5581 tgatctgaaa ccggccaata ttttcctctc caagaacgac gacgacgagc ccggatggac
     5641 ggcaaaagtg ctggatttcg gggtcgccaa gctgagtgaa gagcgcgatc ggctcaccac
     5701 gcagcccggc accgtgctcg gaacgccgct gttcatgagc gcggagcaag tgcgcggcgc
     5761 gagcagcgtc gaccatcgcg cggatctgta ctcgctcggc atgtgtttct atcacatgct
     5821 gaccgggcaa tacgcgttct tcggtgagac gttcaatgac gtgctcgtcg cgatctgcac
     5881 ggagcccctg ccgagtttgc gcacggcggc accccaggtg ccagcggtgt atgaagcctt
     5941 ctatctacgc tgctgcgcgc gtgatccgcg gggccgctac caatcagccc gggagttgct
     6001 cgaagcgctc gaacgtaccg ccaacgaagc gctggcccac agcgccggcc cccacgcgct
     6061 cggcgcccaa gcgctgaaag ccagcttgga tgagcgactg ggcgcgagcg acggcgcggg
     6121 cctatctaga acccaagacg aagaagcacc accgcgatcg ctgaaccgag atgcgttgtt
     6181 cggtgcagac gagtcgggtg actacgcaca acccgcggcg ggcccgttct caccctctcc
     6241 tgttacgctg cgggccgcga cgacggcggc cgaagcactc accgagaggg caccatcctt
     6301 cgatctgcga gaggcagtcg cgggcccatt cgctacgccg ccgcccgcgc cagctcgcga
     6361 gaccgagctg cggcatagcg atgggggggc ctcgattacg gtgcggcggg ttcggggcac
     6421 gccggtgcgg gtcggtgttt ggggcctagg cgtcgtggcc ctcgtcgcgg ccagcttcgt
     6481 aggcatcaag ctctggcgca atcgcgatgc gctaccgcgc gcgccggtcg cggccagctt
     6541 gcacgaagtg cgcgcgaaca cggcggacgg cgtaccctcg agtaccgaac caacgcctca
     6601 tatcgagttg gtgccggagg aatcggcggc gcatgacctt acgaacgcgc agcgcacctt
     6661 gcgcgctgaa atcgcgcagc cgaaagcggc gcttgctacc ccgacggcta gctccacctc
     6721 gacttcatca ccgccgctcc ccgctcgcgg ttcgcacaaa cccaactcgt ccgagccacc
     6781 gccttcgaac gatcgcgctg ccattcccgc tgcgactccc gccatgaaca acggatcggc
     6841 cgctgcccca aagcccccgg ccccggaagt cgatatcggg ttctgaacgc cggccccgaa
     6901 tggtcatcac ctgaacaggc tttcggccta ggttggacct acacgaccgc agagcccccg
     6961 cccgtgtgcg ctcgtccaac cagggctact ttccaggcgt atgcggcctg cgggatcgtg
     7021 atagggtccg cctggtagac acatctggag ggctttcttg atgggatcgc gctcgggcgg
     7081 ttcgcggagt cagcttgggt tgctccgtca ctctcgaggc gatcgagcag ctgagttcga
     7141 atagttcgca actcaaagcg attggtggcc gaccttcgaa tgcgggtgat tcgaaggtca
     7201 tgagccattc aggagcgagt ggcagggatc gtggcgatcg accgtcactc cgaccgaaga
     7261 cacagtcatg acagaacaca aagcttcgcc gctgcgggtc ggcacgctgc tcttggggcg
     7321 ctacgaagta cgggcttgcc tgggtcgtgg cgggcacgcg cacgtttacg aggcttatga
     7381 tgcgttcgtg gatcgcgccg tcgcgatcaa agtgatcggt ccgagcgaga gcggcagcca
     7441 agagttgctc aggcgagcgc aatcggaggc ccgcgtcctg gcgcggctcc agcacccgaa
     7501 catcgttcgg atgtacgaag ccggcatgac cgatgagggg ttcgtatatc tggtcatgga
     7561 gaagctcgag gggcgcacgt tgctggatgt ctcgcgagac ccggggcggc tgcgaccgag
     7621 ggaggccttg gagatcgcgg ttcagatcgc ggatgcgctg ctggtggccc acgtccaagg
     7681 cgtgctgcat cgcgatttga agcctgcgaa cgtgttcgtt accgccgaca accgcatcaa
     7741 ggtgctggat ttcggggtcg ccaagatcaa ggactacggc gcgcattcca cgcggcggga
     7801 cgtgatccag ggcacgatga tctacatggc cccggagcaa ctccaaggcc tgacgctcac
     7861 gcctcggagc gacatcttcg cgctcggcac catgttgtac gagcttttgt atcggcaccc
     7921 ggtgctgctg ggcgagtgcg tgccgagctt tgccgaggtg ggctggctgc aaattgcgac
     7981 cttgcctccg atgctagaca ctctggatcc cagcatcccg cgttatgtcg cgcgcttcgt
     8041 gcaacgttgc ttattgaagc gaccggaaga tcgcttcgag agcatgctgc aggtggccga
     8101 agctgcacgc accgcgctcg agcggatgca acacgagggc aagccagagc tcttgagcct
     8161 gagagagctc agcttgccga cgggccttgc catcgaacag caagcaacct tgcacgcttc
     8221 gcaaaagctc acgctgcgtc cttatctgcc gactgcagaa cccgagctcg agaaagcctc
     8281 gaatgcgcgg ctcaggcctg gattggagat ccacggcgag cacgaaccaa gggccggtgc
     8341 gcgaaacggc ccgggtcgaa cgttacgcat tttgaatggg cccttggctc cgccccaagc
     8401 ctcgttcgtc catccggttg caacccaatc cacccgcact gcgagccccc gctggcaggc
     8461 gccccgcgcg gtcgaggccg cgccggtgga tcccaacgcg ctcgggagcg ttacggaaac
     8521 cggcctcgtt accaaagcac ggcgcgcgct cgggccacaa aagggagtcc gcaaaagcgt
     8581 ctacttgttc gtcctggttg gagtggcttt cttcagctat tggcaagctg cgggtcgcga
     8641 tcagggaacg cggccccccc tcgacgaggt gttggagaaa tcggtcgtga ccaaacgggt
     8701 gcttgctcaa aacctcgaag gccagccgag ttcgcgtccg gcctcggcgg tgacggtatc
     8761 cgcggcgatg gctgctgagt cggctccacc cgcggagcct tcggcggaac aggaagctca
     8821 gccatcagcg agccctcgag cgaagcccag tcaggtttcg gaacgcgcgg aagcattgcc
     8881 ggatcccgcg gccgcggcca aggtccctcg agtggtcacc aaatcagccc gcagtacgcg
     8941 tgaccgagcc gccacgctct tcgacctccc attcgctctg cccgacgatg aacccgcgag
     9001 tagcttggaa gcttgggcca acagcagccc tccgaagcct gcctcagcga acaaaccctc
     9061 gcgtgctgcg gcacgaagtg ccggattgat cgccgatcca gtagacgagg tttacgggcc
     9121 cagcacggtg agtcctgctc agccgagtcg cggtgctgtg cagcgaggtg ccccatgaca
     9181 gacaaccgcc gttatccacc gccgctccca gccgagcctt cgccagagtt ctctccggtc
     9241 gaaccacagc gtgtgccgct cggcatgggc gcccgcgcgg tgcgccccgc accgaagaag
     9301 ccccatggct tcgtgccgcc ggcgagcaac accgcgccag cgagcccggc cgcgaagaac
     9361 caggggaaca gctcgccgtc ggccaacagc acggcggtcc cggcaggccc ggctcctact
     9421 ggagccggtg tcccgaatgc ctcggcgacc actcgcggcc ctgggcttct ctcgttgcaa
     9481 cgctcccaag aggtcgcgct gcccgctgga attcgccaaa aatggctaca gttccaagcc
     9541 cctcgcgtga acccggggga gttccaggac accgttcagc caacgactgc gggcggcgcg
     9601 accggagctt ccaccgctca aggagcggcc ggagtcaccg ccccgcaaac tcccatggcc
     9661 tggcccacgc ccgcggcgag tgccaacgcg gccgccgccc atgcagccaa gagcgcggca
     9721 gcgagtcaga agtcgggctc tgccaaggcg cccggcaaag cggacggcaa gaacccggcc
     9781 cgctcatcgg ctcaggtccg gctctctcct tggctcgtca gtggtctgtt tttgatcgcg
     9841 gcaggcggcg gttggttcgg gattcgcagc ttcgtgggcc gcctcgggag ttcgtcggcg
     9901 cgtttggaaa cggccaccaa gagctcgaag cccgcggcgg gcgtcgtgag cggtgtcgcc
     9961 atcgcggccg ccaagccagg tgagggaaac ccaggtgagg gaaacccagg tgagggaaac
    10021 ccaagtgagg ccggtgaacc ggcgaccgcc ccaaccccgg aggcaacccg cccgggcgca
    10081 gacggagtga acgccgaagt tgcagttgca cccgctcagc gaaacgcggc caaaccgtcg
    10141 gcgcaagcga ccaagcccga ggtgcggggt gctcgagcgg aaggctcggc aacggcggac
    10201 tcgagggtgg cggagagtgc caccgaagcg ccggtgacga gcccgcctgt acaatctcgt
    10261 gagcctcatc gttccccggc tcgcaacagc aaagccacca aacgaagcag cgttctcgac
    10321 acgccgatca tcgaccccga tggctgacat cgtttctgat atgaacgcac cacgatcgcg
    10381 gatggctggt gctccagcgc gtactcaccg gaagattttc gtgaaaagct ctcctcactc
    10441 gagacatcag agccgtaaga tgttccacgt cgtggcgctt tgcggggcct ttacgggtct
    10501 cgtgaccgca acccacagcg cgcgcgcaca acccggcgct gccgcacaac ccggcgctgc
    10561 cgcacagccc aatccgtcag cgagcccgag cggccccaaa gctacgtccg cgacttctgc
    10621 cgcgggtacg gcgtccccag gcggcgcgaa ttcaggaacg ccaggctcgg gcgtgaccgg
    10681 ttcgaacgaa tacgctcagt tccgcaaagc ccatgacgcc atgaaggcgg agaactggca
    10741 ggaagcgcgg cgcatcttgc tggggctgtg ggacaagtcg aatagctacg acgtgggcgc
    10801 gttgctcggt caggtagagt tcaagctcgg gcactacggg ctcgcgacgc ggcgcatggc
    10861 attcgcactg gcgcacatcc cacccaaaga aaaaccagag acgcgggagc gcttgcaggc
    10921 tgcgttcaaa gaaatccgct cgcgcgccgg agcgctgcgc gtggccgtgg ccagccccaa
    10981 cttacgcgtg ttcgtggata acgagaacgt ggggagtacc ccgttcgaca gcgaggttta
    11041 cgtcgatccc ggcacgcacc tggtcgaact gaaggacgca accgatcgcg ccgtcgcttc
    11101 caatcgaaat gcctcggtcg cggcgggtca ggtggccgtg gtcgatctgc cgatcacggc
    11161 ccgggacgct gggggcaccg gagtgctttc gaacccgggg gaacccactc ccataagcgg
    11221 ggcggagtcg tcgaacggga gtggcgtcga aagtaacagc cgcctgccgc cagtcgggcc
    11281 ggagcgacac aagagcctcg ttccggttta tgtggcgggt ggagtggcag cggctgggct
    11341 cgcagtagca attgggttcg gcgtcgcctc gcgcagtgcc aagaacaaag ccgaggacca
    11401 ccaggcacta cttggcccct cgggctgcag tggcggtggt tcgccggctt gcgatgaact
    11461 tcgagatgaa cgggatgcgt ggcgaagaga cgcgcgcatc gcgaacattg gctgggccgc
    11521 gtttggcgtc ggcgtagccg cggcgggggt gtggctcttg ctccctggcc cacgccacga
    11581 aaccggagcc caggccagcc aggggctagc gcttcgcgct gacgtgcagc ccgggcctca
    11641 gggtgcacgc ttctccctgg gagggcagtt ctagtaagct cgcgcgagct ctagggaccg
    11701 agctctttca agaacttcaa ccgcggaaag ctgcaaagcc gaaagccgaa gaggctgaag
    11761 cgctccgatc gttcggatcc atcgtgcagc ccagattcta agcaagctga accgagtgtg
    11821 agcgggatat gagatattca aagattcagt ggctgtgtgg aagcgctctg ttgtcgctcg
    11881 gcattt
//
LOCUS       DASTKU010000014         4149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_447980, whole genome shotgun sequence.
ACCESSION   DASTKU010000014 DASTKU010000000
VERSION     DASTKU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447980"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2531)
                     /locus_tag="VFQ61_00455"
     CDS             complement(<1..2531)
                     /locus_tag="VFQ61_00455"
                     /inference="COORDINATES: protein motif:HMM:NF015102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HET9952935.1"
                     /translation="MTEKMWFSGGGQRVREAVRSALDWCELCIVVTDGSRATPEDAFW
                     SQLGAEAAKVRQLLLTGGSLPEPWLRTRFDGPGQLRSLEGPIRGHFIWFERGGEVRFL
                     LAPDQLCLSTVTAGGLWMHWEGSGSALPDGLGELFAECCTASREVERATQPRPSPRTD
                     EAQQETQPSDDSRAAPEPYSRAIPSVREVLGIRSTLDFSDLDEEAQALALWSTLIGEG
                     AVDLEQAIRLSAERLRAQGFLEYQLLRQDGRIYGLLESRLLAARRSAYLFDRPQNGLV
                     RAIQPNLDELTAEQWRDCVLGALPIHTRVDREHAVRIGFTYAQLVYGIDAQRLRSGGR
                     ADQALRSAINSAIRQGHLERDGAAYLIRSSETSAPTQRGALAPAAESAPPPSAPASKV
                     EASQAAASPPSPSVSQSDAERASRVVATDPAQGGLGGGMPRGAEPGPSEWVSDHPPSS
                     RASTRNVTPSSALDTKLLDLKLPTRAQNWAERQGIDTLRGLVAWDPEAFVKERNVGRR
                     TVQETRELLEARLGCSWESAHSALRQGLEPEPGSSPENEDVDAATEALTSGGASGWAE
                     LARRLTSEQRANSLREIDLPARMRTFVEQEELTTLGELFRFRYEELRSRPNLGRKTLN
                     DTLDAVRESLNEQAAPPQYTSFLQSWQAQLLALEPIPRMIVTRRAGMYGTRETLEDVG
                     AMLGVTRERVRQIEAHVVDRLRERSPWTRVVAQKLAEAFGAARALPVALLAQDLWWAG
                     IGEQPLLLEFVIRRVFEDQYFLLEVPNGKLYLTRFPPSVFMEHVESAKARLGKLEYPV
                     ELAVVQQILQSETDALDPVLFSEFETLAAEWVLRDSSRPDFALGYG"
     gene            3180..>4149
                     /locus_tag="VFQ61_00460"
     CDS             3180..>4149
                     /locus_tag="VFQ61_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952936.1"
                     /translation="MDDAATPTESPYERRILPPGIYLAITTIGLAFGLRLTSVFPSGL
                     ERDVLAILTEAIPWFRNSYPALAGMLAVSIVLLLVVTTLVATAVADYIEALLVENALR
                     GTEASVSAADDAASSLGTHSTNPAGPGWLWDGMLANSFGRATRFAHYSASWQLLVWTI
                     VVALWVAFPRLPWWAGAALTVLSTWRTSTGLLGKGMSLFSALVGTLLFLFAMVLHDYA
                     VKPKTESIDGFLTFGKGSAEWIDCGSRQREAIELSAMVRGWNKVMRAAEEAKSMLGCP
                     AGDPVFCPAPVVHVVGRAYRRVGSRGADNQYSGLVSFISIEKAEPNA"
BASE COUNT          783 a         1289 c         1274 g          803 t
ORIGIN      
        1 ccgtaaccca gcgcgaagtc cggtcggcta ctatcgcgta gcacccactc tgctgccaaa
       61 gtctcgaatt cagagaacaa caccgggtcc agagcgtctg tctccgactg gaggatctgc
      121 tgcaccacgg ccagttccac gggatactcc agcttcccca acctcgcttt cgcactctcc
      181 acatgttcca tgaacacgct gggaggaaac cgggtcagat acagtttccc attcggtact
      241 tccaacaaga agtactgatc ttcgaagact cggcgaatca caaactccag gagcagcggc
      301 tgttccccaa tgcccgccca ccacaggtct tgtgctaata aggcgacggg caaggcacgc
      361 gccgctccga aagcctcggc gagtttctgc gcgaccactc tcgtccacgg cgagcgctca
      421 cgcaaacgat cgacgacgtg agcttcgatt tgacggacgc gctcccgagt cacgcccagc
      481 atcgcgccga catcctcgag cgtttcgcgc gttccgtaca tcccggcgcg tcgagtgaca
      541 atcatgcgcg gaatgggttc gagtgccagt agttgagcct gccacgactg cagaaatgag
      601 gtgtattgcg gcggcgccgc ttgctcgttc aagctctcgc gcaccgcgtc cagcgtgtca
      661 ttcagggtct ttcgtcctaa gttcggtcgg ctccgcagtt cctcgtatcg aaagcggaag
      721 agctcaccca gtgtcgtcag ctcctcctgt tccacaaagg ttctcatccg ggcgggtagg
      781 tcgatctcgc ggagcgagtt ggcgcgctgc tcactggtca ggcgacgtgc gagctccgcc
      841 caacctgagg ctccgcccga ggtgagggct tccgtggcag catcaacgtc ttcgttctct
      901 ggagaactgc ctggttcggg ttccaaacct tgacggagag ccgagtgcgc cgactcccaa
      961 ctacaaccca ggcgggcctc gagcaactcc cgagtctctt gcaccgtgcg gcggcccacg
     1021 ttgcgctcct tgacaaacgc ctcagggtcc cacgccacga gccctcgcag cgtatcgatg
     1081 ccttgtcgct ctgcccagtt ctgcgcgcgc gttggcaact tcaaatcgag tagcttggtg
     1141 tcgagcgcgg aggaaggagt cacgtttcgg gtgctcgcgc gactcgaagg cgggtggtcc
     1201 gaaacccact ccgaaggtcc cggttcagcg cccctgggca tgccccctcc gagcccgcct
     1261 tgggcaggat cggtcgcaac gacgcgggaa gcgcgctcag catccgattg tgaaacggat
     1321 ggactcggcg gggaggccgc cgcctgcgat gcctccacct tgctcgcggg cgcgctgggg
     1381 ggaggtgcgg actctgcggc gggcgcgagg gccccgcgtt gagtcggcgc agaagtctcc
     1441 gaggagcgta tcaagtacgc tgctccgtcg cgctccaaat gaccctgacg gatggcgctg
     1501 ttgattgcgc tccgcaaggc ttgatccgcg cgcccgccag agcgcaatcg ctgcgcatcg
     1561 atgccataga ccaattgcgc gtacgtgaac ccaatccgca ccgcatgctc tcggtcgacc
     1621 cgggtgtgga tcggtagcgc acccaaaacg cagtcgcgcc attgctccgc ggtcagctca
     1681 tcgaggttag gctgaatcgc gcggactagg ccgttctggg gccggtcgaa cagataggcg
     1741 ctccgccgcg ccgccaatag ccgactctcg agaagaccgt agatgcggcc gtcttgccgc
     1801 agcagctggt actccaggaa cccctgtgca cggagccgct ccgcactgag tcgaatggct
     1861 tgctccaaat cgacagctcc ctcaccaatc aacgtgctcc acagcgccag agcctgtgcc
     1921 tcttcgtcga gatcggaaaa gtcgagggtg ctgcgaatgc ccagcacctc ccgaacggag
     1981 ggaatcgcgc gcgagtaggg ttctggggct gcgcgggagt cgtccgaggg ttgggtttct
     2041 tgctgcgcct cgtcagttcg cggagacggc ctgggttgcg tggcgcgctc cacttcgcgg
     2101 gaggcagtgc aacactcagc gaagagctcg ccgagcccgt ccggcagagc gctccctgaa
     2161 ccttcccagt gcatccacag gcctccggcc gtcacggtgc tcaggcagag ctggtcggga
     2221 gcgagtagga agcggacctc acctccacgc tcgaaccaga tgaagtgacc tcgaatcggc
     2281 ccttccaagg accggagttg cccgggtccg tcgaatcgtg tgcgcaacca aggctcgggg
     2341 agcgagcctc cggtcaggag cagctgacgc accttcgcgg cttcggcccc cagctgagac
     2401 caaaatgcat cctccggcgt ggcgcgggaa ccatctgtga cgacgataca gagctcgcac
     2461 caatccaacg ccgagcgcac cgcttcccgg acacgctgcc caccaccaga aaaccacatc
     2521 ttttccgtca ttcgcgagcc cgacccgttg gactagctga gcagcatagc gcggctgcac
     2581 cagtgttcgg caagcgggaa caagttggaa tgcctgcagc gacttcggtc atgccgagcg
     2641 tctgacctgt cgcgagggcg gaggtgtaca ggtggcacac atgacggaag cggtcgggcg
     2701 gcgtcgtcag tgtcgccagc tggcgagtga ccggtcaagt ccttgcgctg agcaaacgga
     2761 gtctggttcg acacgttgag accccgaatt aattaccatt ggggcgctag ccaaacaccc
     2821 gctacaacat tcggagagaa gggttcagcg gggcagtggg gagacgggct tcgatgttcg
     2881 agcaactcgg cgtgcagaca gtgattgcag cctgagctcg atgcgattga gtaaaaaatc
     2941 ccgcagcgcc gtcaacatat cgaatgagaa acgcagagca agatgatgcg gaacgattga
     3001 gtcgtcccga tatttggggg tggaatagct atgttgaaca ggcaatccag ttgcccactc
     3061 gctttctttg gacgtcgcta ctgtgcttag tcgctcccgg gatgctgttc cgggtagaac
     3121 gggtcatccc gccgccgcgc gatgaccgag acgtcagcaa aggtgctcca cgcatacccg
     3181 tggacgatgc tgcaacgccg actgaaagtc cttatgagcg acgcatcctt ccacccggga
     3241 tatatctcgc gatcacaact atcggcttag ctttcgggct tcgcctgacg tctgtgttcc
     3301 cgtccggact ggaacgcgat gtgttggcaa tcctcacgga agctattccg tggtttagaa
     3361 acagctaccc tgctcttgcc ggaatgttgg ccgtgagcat cgttctcttg ctcgtggtga
     3421 caaccctcgt ggcgactgca gtagccgatt acattgaggc gctcttggtt gaaaacgccc
     3481 tacgcggcac agaggcgtca gtgagtgcgg ccgacgatgc tgcctcatca ctcggcacgc
     3541 attcgacgaa cccggctggc ccgggctggc tttgggacgg tatgctggcg aactcgttcg
     3601 ggcgggccac tcgatttgct cactactctg cgagctggca gctgctcgtc tggacgatag
     3661 tcgtggctct gtgggtcgcc tttccgcgtt taccttggtg ggctggcgca gctttgacgg
     3721 tcctctctac gtggcgcacg tcgacaggac ttttgggcaa aggaatgagc ctattctctg
     3781 cactcgtcgg aactttactc ttccttttcg cgatggtctt gcacgactac gcagtgaagc
     3841 ccaagaccga atcgatagat ggattcttga ccttcgggaa gggatccgcc gagtggattg
     3901 actgcggatc ccgccagcgc gaggccatcg aattgagtgc gatggtgagg ggttggaaca
     3961 aggtaatgag agcggcagag gaggctaaat caatgcttgg ctgtccggcg ggggatccgg
     4021 tgttctgtcc ggcgcccgta gttcacgtcg tcggaagggc gtaccgtcgc gtgggctccc
     4081 gcggtgcgga taaccagtac tccgggctgg tctcgttcat ttccatcgag aaagccgagc
     4141 ccaatgcgc
//
LOCUS       DASTKU010000015         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_224934, whole genome shotgun sequence.
ACCESSION   DASTKU010000015 DASTKU010000000
VERSION     DASTKU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224934"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFQ61_00465"
     CDS             <1..205
                     /locus_tag="VFQ61_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952937.1"
                     /translation="ESYHLPDEVLLSSPVRLPDLAGSRSFDALEPARHERERPNSRPE
                     PDRQWKLARVISSEAKERCLPLL"
     gene            complement(270..908)
                     /locus_tag="VFQ61_00470"
     CDS             complement(270..908)
                     /locus_tag="VFQ61_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952938.1"
                     /translation="MLISEHTLRPADIIVSTTDASISAVIRAGTGSSVSHSMIYLGGS
                     FVIEAIAEGVVRRPLAVALEQAALAIALRRRNLTDQQRRAVIDYATGFMFRPYDEIGA
                     AGAATSLGRGSLLAAFGCGISLGLCALGVVGMANNAKPANADRAFFCSELVARVFELA
                     GAPVVSGPPSYTTPRQIRTADTLLYLGKLVDESPRVGARLGPARGAEGARAF"
     gene            complement(922..1398)
                     /locus_tag="VFQ61_00475"
     CDS             complement(922..1398)
                     /locus_tag="VFQ61_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952939.1"
                     /translation="MKAGQSRAPSRWLGASAVILLGLLGSACSIEQPVSGPIQLTGEW
                     TSITPPEPLRVAGKLEQKVCLQIVGTVTDIDLGNGVMLDGQRHVLGGAAIDNEQVEYT
                     LGVGELGPRGVCLYRPPQDTPPGPDFPAQRTIIGLRLRSDPPLRVGAIRWRSYDPF"
     gene            1669..>2012
                     /locus_tag="VFQ61_00480"
     CDS             1669..>2012
                     /locus_tag="VFQ61_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952940.1"
                     /translation="MQRLPSTAPRPITSWRAAAPGTFLAVALVACGASHDPDHQPFAA
                     DWDGVGWTATTTRDAPPDAGPGVNEAQPCDEEGARIECGRVTERHADYVTCSMGHRTC
                     TDGVWSACVGERS"
BASE COUNT          358 a          653 c          655 g          346 t
ORIGIN      
        1 cgagagctat catttgccgg atgaggtgtt gcttagctcg cctgtgcgct tacccgatct
       61 ggctgggtct cgttcttttg acgcgttgga gccagcgcgc catgagcggg aacggcccaa
      121 ctcacgccct gagccggacc gtcaatggaa gctagcccgg gtgatcagca gcgaagcgaa
      181 ggagcggtgc ctgccgcttc tgtagggcga gcaggtagcg agcctcgtcg tagggggtgc
      241 gttcgaccca acagcggtgg aggatgcgtc taaaaagcgc gggccccctc agctcctcga
      301 gctggcccga gtcgcgcgcc aacgcgaggg ctctcgtcca ccagcttccc caagtacaat
      361 agcgtgtccg ccgtgcgaat ttgccgcggc gtcgtgtagg aagggggacc cgagacgacc
      421 ggagctcccg ccaactcgaa gactctcgct acgagctccg agcaaaaaaa cgctcggtcc
      481 gcatttgcgg gcttggcgtt gttcgccatt ccaacgaccc cgagcgcgca gagcccgagg
      541 ctgatcccgc acccgaacgc cgccagcaag ctgccccggc ccagactcgt cgccgcgccg
      601 gccgctccaa tctcgtcata aggccgaaac atgaaccccg tcgcgtagtc gatcacggcg
      661 cgcctctgtt gatcggtcag attgcgacgg cgcaaggcga tcgccagagc ggcttgctcg
      721 agcgcgaccg cgagcgggcg gcggacgact ccctccgcga tggcctcgat gacgaaggag
      781 ccgccgagat aaatcatgga atgactgacc gatgatccgg tccccgcccg aatcacggcg
      841 gaaatactgg catctgtggt gcttacgatg atgtcggcgg gacgcaatgt gtgctcggag
      901 atcagcatgg tctttctcgt ttcagaaggg atcgtaggaa cgccagcgga ttgccccgac
      961 tcggagtggt gggtcgctcc gcagccgaag gccgatgatg gtacgctgcg cggggaagtc
     1021 gggccccggc ggggtgtctt gggggggacg gtaaaggcaa accccacggg gccccagctc
     1081 cccaaccccg agcgtgtact ccacctgctc gttgtcgatg gccgcgcccc ccagcacgtg
     1141 ccgctgaccg tcgagcatca caccattgcc gaggtcgatg tccgtcaccg tgcccacgat
     1201 ctgtaggcag accttctgct cgagcttccc ggccacgcgc agcggctcgg ggggcgtgat
     1261 gctggtccat tcacccgtca actggatggg cccggacacc ggttgctcga tggagcatgc
     1321 agacccgagc agccccaaga ggatgacggc ggacgcaccg agccagcgcg atggagcgcg
     1381 tgattgcccg gccttcattg cggacctctt gcctgcgcgg ctcctcggga atcaccccca
     1441 gtggtttgca tacgaatgct cttcggcctg gtagagaagg aattgtcggt tttctccgga
     1501 aagtcatcgg ccggaaaatg gctgcgattt cgcgcgcggc gacgggcgcg agctcgctcg
     1561 ggcacgttct gcctccgagc gaagccccga aacgatacgc cattcgctcc gttaaagcat
     1621 cctgggtgta ctcttttgcc gcaagattca cggtaaattg aatacgcgat gcaacgcctt
     1681 ccgagcactg caccacgtcc gatcaccagc tggcgcgcgg cggcgccggg aacctttctg
     1741 gccgttgcgc tcgtagcatg cggcgcgagc cacgatcccg atcaccagcc cttcgccgcc
     1801 gattgggacg gcgtcggctg gaccgcgacc accacccgtg atgctccgcc ggatgccggt
     1861 cccggggtga acgaggcgca gccctgcgac gaagagggag cgcgtatcga atgcggacgt
     1921 gtcacggagc ggcacgccga ctacgtcacg tgctcgatgg gtcatcgcac ctgtacggat
     1981 ggtgtttgga gcgcctgcgt cggcgagcgc tc
//
LOCUS       DASTKU010000016         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_894376, whole genome shotgun sequence.
ACCESSION   DASTKU010000016 DASTKU010000000
VERSION     DASTKU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894376"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFQ61_00485"
     CDS             <1..451
                     /locus_tag="VFQ61_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952941.1"
                     /translation="FKALGVAIIGLAATFVAPLGWTTRHYGLTVTWASLLFTAMLGLT
                     VTARPGSWLERLFGSPVLRFAGFYSYGLYLFHEPIFRELGTHLPTRSGPAIVHWLALA
                     VTGTALSVLLAMLVYHGYEARFLSLKRFFPTGRPAAPSTLAYPAKES"
     gene            complement(556..>2413)
                     /locus_tag="VFQ61_00490"
     CDS             complement(556..>2413)
                     /locus_tag="VFQ61_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HET9952942.1"
                     /translation="PACLAAALAKHPSVFEIGVAYEPFRGDLHARLLAPHAARVDGAV
                     QSFELADRYDYTERDWYKLGLKGAAWGEPYFGAATKRQVVGYTEPFTLPGDPTKTVIG
                     VVRVNLALDGVASLVSSVSLGRTGYGYLISGNGTYIAHQNPEYARLGRNAYDVARELK
                     DEQRVHNLDRVLKQREATEQVVTNALTGQSAWLFNQPLALNSWVVSTVSFRDEVRFDE
                     RDTRRAVIQVMSWLLLAGFGASVLLFRAYRGAHRELWRATLSAASLLLVGIFVMWALT
                     LRFPDRNGEESVHIMEATALHKFLANQRELTEPAGEQIRIAAGLLVRTMRSTSANDYV
                     ITGTVWERIPSDRLPEIHSGLDFPDAESVELRESSTVRQGENELISWNFKVALREPSK
                     WSEKYPFDRALIRIRMTPKPSKVPVVLVPDLLGYQLLTPGELPGVEKALALPGWALDH
                     SYFSFLPRAKDYNTALLKLQNQPLPYVLSFNIVTQRRFLDPFIASVLPLIVIFSLLYG
                     LLIVGSKVSTKVAATGFKATDVLRASVGLLFPALIAQVNLRTKIGATEIIYIEYFYFI
                     LYAAILGVAANALTFTLSREGVSQVRDNLVPKLLYWPLVLGMCFALTFVFLY"
BASE COUNT          478 a          753 c          723 g          459 t
ORIGIN      
        1 gttcaaagct ttgggcgtcg ccataatcgg cctcgccgcc accttcgtgg ctcccctcgg
       61 ctggaccacg aggcactacg gcctgaccgt gacatgggcg agcctgctgt tcacggcgat
      121 gcttggcctg accgtcaccg cacgccccgg gtcctggctc gagcggcttt ttggtagccc
      181 ggtgctccgc ttcgcgggct tctacagcta cgggttgtac ctgttccacg aaccgatctt
      241 ccgcgaactc gggactcacc tgccgacccg aagcgggccc gcgatagtcc attggctcgc
      301 gctggccgtt accggcaccg cgctcagcgt gcttttggca atgttggtct accacggcta
      361 cgaagcgcgt tttctgtcac tcaagcgctt cttcccaacc ggaaggcccg ctgcaccgag
      421 cacgctcgcg tacccagcga aagaatcatg aagcaagcgc caatcgcgcg gcgcagctat
      481 cgaacgcgac tcacgagtcg tgtgtgcaga aaaagcaggg gaagcttgtt gcataccccg
      541 gccgtcgtgg ctccctcagt acaagaagac gaacgtcagg gcgaaacaca tccctaagac
      601 gagtggccaa tacaggagct tgggtaccag gttatctcgc acctgagaga cgccttctct
      661 gctcagggtg aacgtcaagg cgttcgcagc aacacccaag attgctgcgt acaggatgaa
      721 gtagaagtac tcgatgtaaa tgatctcggt tgcgccgatc ttcgttcgga gattgacctg
      781 cgcgatcagg gccgggaaga gcaggcccac gctggcgcgg agcacgtctg tcgctttgaa
      841 gccggtcgcc gccaccttcg tgctgacctt ggatccaacg atcaagagcc cgtagaggag
      901 ggagaagatc acgatgagcg gcagtaccga cgcgatgaaa gggtcgagga accgccgctg
      961 cgtcacgatg ttgaaactca agacgtacgg cagtggctga ttctggagct taagcaacgc
     1021 cgtgttgtag tccttggctc ggggcaggaa actgaaatag ctatggtcca gcgcccaacc
     1081 cggcagcgcg agcgcctttt ccacaccagg aagttcgcct ggcgtcagca gttggtagcc
     1141 aagcagatct ggcacgagaa cgaccggcac cttggatggc ttcggcgtca tgcgaatgcg
     1201 aatgagcgcg cggtcgaaag ggtacttttc cgaccatttc gagggctctc gcagcgccac
     1261 cttgaagttc cagctgatga gctcattctc accttgtcgg acggtactgc tctcccgcag
     1321 ctccacggat tctgcgtctg gaaagtcgag accggagtgg atttcgggta gtcggtccga
     1381 cggaatccgt tcccagacgg tgccggtgat cacgtagtcg ttggcgctgg tggagcgcat
     1441 ggtgcgcacc aatagtcccg ccgcgatccg gatctgttcg ccagcgggtt ccgtcagctc
     1501 ccgctgattg gccaggaatt tgtggagggc ggtggcctcc ataatgtgga cactctcctc
     1561 tccgttgcga tcggggaacc gcaaggtgag cgcccacatc acaaagattc cgacgaggag
     1621 cagggatgcg gcggacagcg tcgcccgcca cagttcgcgg tgcgcgccgc ggtaggcacg
     1681 aaataagagc acgctggccc cgaagcccgc caacaaaagc cagctcatca cttgtatcac
     1741 ggctcgtcgc gtgtcgcgct cgtcgaagcg cacctcatct cgaaacgaca ccgtgctgac
     1801 cacccagctg ttgagcgcca gcggttgatt gaagagccaa gccgattgac ccgttagggc
     1861 attggtcacc acctgttccg tggcctcgcg ttgcttcaga acgcggtcga ggttgtgtac
     1921 ccgctgctca tccttcaact cgcgcgccac gtcgtaagcg ttgcgcccaa ggcgcgcgta
     1981 ttcggggttt tgatgggcaa tgtaggtccc gttgcccgaa atcagatagc cgtagcccgt
     2041 ccgtcccaag ctcacgctgg agacgagcga tgcaacgcca tcgagtgcca gattgacccg
     2101 caccacccca atgacggtct tcgtcggatc cccgggcaga gtaaatggct ccgtatagcc
     2161 tactacctgc cgtttcgtgg ccgcgccgaa gtacggctct ccccacgccg cacccttcaa
     2221 gccgagcttg taccagtcgc gctctgtata atcataccga tccgcgagtt cgaaggattg
     2281 caccgctcca tccacgcgcg ccgcgtgcgg cgcaagcaaa cgcgcgtgaa gatcgccacg
     2341 gaacggttcg taagcgacgc caatctcgaa tacggacgga tgcttggcga gtgccgcggc
     2401 caggcacgcc ggc
//
LOCUS       DASTKU010000017         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_448730, whole genome shotgun sequence.
ACCESSION   DASTKU010000017 DASTKU010000000
VERSION     DASTKU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448730"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFQ61_00495"
     CDS             <1..767
                     /locus_tag="VFQ61_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952943.1"
                     /translation="RVVSRGLVMQSIGFRARSGTSWGRPVGMALSWTAALLVACHSEG
                     CRNRGSAAQIAQCERLCTQLVTPVGCGGLEGTDSLVERCSVACGEGAEQALTAGCEQA
                     HTGYLQCLSRQSESCSESDRSAASKLESAPARSSCAREFDRLARCRSVCDEAAVVYTK
                     TGRVTVNGVDRDLSGEAVGLGCGSREPQNARRAPAGARCEHHSVCSRERCTCPGRRLG
                     FLARACVDGQCADRKQACEWVPKLLPQYGCGGPTLP"
     gene            complement(775..1302)
                     /locus_tag="VFQ61_00500"
     CDS             complement(775..1302)
                     /locus_tag="VFQ61_00500"
                     /inference="COORDINATES: protein motif:HMM:NF002616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HET9952944.1"
                     /translation="MVLRPIMVQPRAGSHSAASSRPDPKLRAWVGVVSRAHVQRGVAG
                     GFAQVCHGKRAPLARMGSGDWFVYYSPTTEFKGGLPLRAFTAIGRIRTDVTYAFDMGE
                     GFVPYRRDIAYEGVLREVPVQELSRELRFIRENPNWGMLARRGLFEIPLEDMRAIALA
                     LLGPAHPALGTLRAK"
     gene            1718..2086
                     /locus_tag="VFQ61_00505"
     CDS             1718..2086
                     /locus_tag="VFQ61_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952945.1"
                     /translation="MSKDSSLTGNVREGIPESLPEELTQTLVTRPNTRIERILSRGQA
                     SPPDFWYDQAQHEFVLLVSGRAILEFETSRGDIESGSERRHVELGPGDWIQIDAHVKH
                     RVHWTDPAQTSVWVVVFYGP"
     gene            2130..2894
                     /locus_tag="VFQ61_00510"
     CDS             2130..2894
                     /locus_tag="VFQ61_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9952946.1"
                     /translation="MGRKRRQVTLEDPARWVFNRMAEHYPARPAYPAALIQQLAGLSP
                     ERRVLDLGAGTGHIALPLARLGFEVTAVDAAGAMVEALRAAATREALPVNALHARAEE
                     VPFERAAFDLVIVADALHFLDVELTAAQVRRVLAPGGSLALVTTEFADTPYMRAVREA
                     MQSAAPRRPRSVAQAVLHLASLSQVDLRDAHSYRDDHPMDVESVEQILRSVSFIGPAM
                     NAVRFARFQAALRAIPYPVVWSREFTLQVGRRRRGP"
     gene            complement(2919..>3492)
                     /locus_tag="VFQ61_00515"
     CDS             complement(2919..>3492)
                     /locus_tag="VFQ61_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952947.1"
                     /translation="STLAGVGFEYRIRARDVGGNVLAEIEGNQPAPPSHHSEPIVRTR
                     DRTAPAPHSSAWAWLPPTLGGAGVLALAASAYFQVQRERAAREWNGPQCERPGQTRIE
                     QCRSVDDRRRQMQTLAVSGAAGGGALLLSGALAFFIEQANSASEQGERARGPRSRTVG
                     VRGIMERPQAPSGAWSCSAGGLGLHCFGQF"
BASE COUNT          615 a         1119 c         1126 g          632 t
ORIGIN      
        1 aaagggtagt gtcgcgcggg ctggtgatgc agtcgatagg atttcgagct cgttccggga
       61 caagctgggg acgtccggtt ggaatggcgt tgagctggac cgcagcactt ttggtcgcgt
      121 gccactcgga aggttgtcgc aataggggca gcgcagccca aattgcacag tgtgagcgcc
      181 tctgcactca gctggtgacg cccgtgggtt gcggtggttt ggagggcacc gactcactcg
      241 tggaacgatg cagcgtcgct tgcggcgaag gcgccgaaca ggcgttgacc gcgggctgtg
      301 agcaagctca cacgggctat ttgcagtgcc tgtcgcgtca aagcgagagt tgcagtgaga
      361 gcgaccgtag cgccgcctcc aaactcgaga gcgcgcccgc gcgatcgagt tgcgcgcggg
      421 aattcgatcg cctcgcgcgg tgccgttccg tgtgtgatga ggcggccgtg gtttacacga
      481 agacgggccg agtgacggta aatggcgtcg accgcgattt gagtggcgaa gcggtgggcc
      541 tcggttgtgg ttcgcgggag ccgcagaatg cacgtcgcgc gcccgccgga gcccgttgcg
      601 agcaccattc ggtctgttcg cgcgagcgtt gcacttgccc ggggcggcga ctgggatttc
      661 tggcgcgagc gtgcgtcgat ggccagtgcg ccgatcgaaa acaagcgtgt gagtgggttc
      721 ccaagctgtt gccgcaatat ggctgcggag gccccaccct cccttgagta ctcgttattt
      781 tgctcgcaga gttcctagcg cggggtgagc tggaccaagc agcgccagcg cgatggccct
      841 catatcttcg agtggtattt cgaagagccc tcgccgagcg agcatgcccc aattcgggtt
      901 ttcacgaatg aaccggagct cgcgggaaag ttcttggacg gggacttcac gcaacacgcc
      961 ttcgtacgcg atgtcgcgac gatagggcac gaaaccttct cccatgtcga aagcataggt
     1021 cacgtccgtc cggattcgtc caattgccgt aaatgctcgc agtggtagcc cgcccttgaa
     1081 ttccgtcgtc ggtgagtaat aaacgaacca gtcgccgctc cccatgcgcg cgagcggcgc
     1141 ccgcttgccg tggcagacct gtgcaaatcc accggctaca ccgcgctgaa catgagcgcg
     1201 agaaactacc cccacccaag ctcggagctt tggatcgggc cgagacgacg cggcggagtg
     1261 agaacccgcc cgaggctgaa ccatgatcgg tcgaagaacc atgcggctct tcctaagccg
     1321 acccggtgac agtttgtgtc agcagttacg ctgacgcggg acggaagagc cgtggtgtcg
     1381 ctgccatcga caacttggca acgatgccca tagcggcgag gcagggcagg acggcctgat
     1441 gcagagccgc acgccgtacc gaacgacgcg ccgagtgggg agccctgccg tggctcgacc
     1501 acggcacacc cctgctacgc cgattgccgc caagatggcc gcgcgcaatg cactcatctc
     1561 tcgcatgact ctcctcctcg tccgaacact tgccggtgcc ggactcccag ccgtagcaca
     1621 cgtgcgacgc cgaacgaagc cgacgttccg gcggccctcg aacgtcagtt tcttctaggt
     1681 cgaggcgcga actcatgaga ggacgacagg agcgcaaatg tcgaaagata gcagcttgac
     1741 gggcaacgtt cgggaaggca ttccggaatc gctcccggag gagctcaccc aaacgctcgt
     1801 tactcgtccc aatacccgca tcgagcgcat tctgtcccgc ggccaagcct cgccgccgga
     1861 cttttggtac gaccaggctc aacacgagtt cgtgctactc gtgagtggtc gcgcaattct
     1921 ggagttcgaa acttcgcgcg gagatatcga gtctggatcc gaaaggcgac acgtagaact
     1981 cggacccggg gattggattc aaatcgatgc ccatgtgaag catcgagtgc actggaccga
     2041 ccctgctcaa actagcgtgt gggtggtcgt gttttatggg ccctgaccgc gtgagtcacc
     2101 ctcgtgagtg cggcacgaag tgattgggca tgggacgtaa acgtcgccag gtaacgctcg
     2161 aagatccggc gcgctgggtc ttcaatcgta tggcggagca ctatcccgct cgacccgcct
     2221 accccgccgc tttgattcag cagcttgcgg gcctcagccc cgagcgccgg gtcttggacc
     2281 tgggcgcggg cacggggcac attgcgttgc cgctcgcccg gcttggcttc gaggtgaccg
     2341 cggtggacgc ggcgggagcc atggtggagg ctctgcgcgc tgccgcgacc cgggaagcgt
     2401 tgcccgtgaa tgcactgcat gccagagccg aggaggtacc tttcgagcgc gcggccttcg
     2461 acttggtcat cgtcgccgat gcgttgcact tcctggatgt cgagctgacg gccgcacaag
     2521 tgcggcgcgt gcttgcgcct gggggcagct tggccctcgt cacgaccgaa tttgcggata
     2581 caccgtacat gcgcgcggtg cgcgaagcca tgcagagcgc cgcgccgcgc cgcccccgct
     2641 ccgtagccca agccgtattg cacttggcca gcctctcgca ggtagatcta cgagacgccc
     2701 actcctatcg agacgatcac ccgatggacg tcgagtcggt ggagcagatc ctacgatcgg
     2761 tttctttcat tgggcccgcc atgaacgccg ttcgctttgc tcggttccag gccgccctcc
     2821 gtgccattcc gtaccccgtc gtttggtcac gcgagttcac tttgcaggtg ggccggcggc
     2881 ggcgaggccc ataacccgtt ccgagcccgt gagtagcttc aaaattgccc gaagcagtgc
     2941 aacccgagcc caccggcgct gcaactccaa gctccgctcg gagcttgcgg acgctccatg
     3001 ataccccgaa ccccgacggt gcggcttcgc ggcccccgcg cacgctcgcc ctgctcactc
     3061 gcggagtttg cctgctcgat gaagaacgcg agcgccccgc tcagcagcaa cgctcccccg
     3121 ccggcggcac cgctcaccgc gagcgtttgc atctgacgcc gtcgatcatc gacactgcga
     3181 cattgctcga tgcgcgtttg cccgggtcgc tcgcattgag ggccgttcca ctcgcgggca
     3241 gcccgctcgc gttgcacctg aaagtaggcc gatgccgcga gcgcgagcac ccctgcgcct
     3301 cccagcgtcg gcgggagcca ggcccaggca ctcgagtgcg gcgccggcgc ggtgcggtcg
     3361 cgagttcgga cgatcggctc cgagtgatgc gacggcggcg ccggctgatt gccctcgatt
     3421 tcggcgagca cattgccgcc cacgtcgcgc gcccgaattc gatattcgaa tccaaccccc
     3481 gcgagcgtcg ag
//
LOCUS       DASTKU010000018         4426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1117749, whole genome shotgun sequence.
ACCESSION   DASTKU010000018 DASTKU010000000
VERSION     DASTKU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117749"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VFQ61_00520"
     CDS             <1..780
                     /locus_tag="VFQ61_00520"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9952948.1"
                     /translation="VRREAAKGFTAIVHLAAMAGVRASVEDPWLYYDVNLTGTLNLLD
                     ASREYGKPNFVFASTSSAYGATRTIPFIETDASDRPLSPYPASKRAAELLGYTYHHLH
                     GADFTALRFFTVYGPRGRPDMMAHKVFDSIRTGKEVPLYNGGQMYRDWTYVGDIVSGV
                     VAAADRRLGFEVVNLGRGEPVLLKDFVSAIEKLCGRTARLTPAPMIDADVSYTFADIS
                     KARRLLGYDPKVSVDEGVRHFHAWYTENVGAFDTGSAPATP"
     gene            777..1898
                     /locus_tag="VFQ61_00525"
     CDS             777..1898
                     /locus_tag="VFQ61_00525"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET9952949.1"
                     /translation="MKVRMLDLAAQHEALEPELSRRVREMMHSGKFILGPAVASFERA
                     AAGYLGVQHAIGASSGSDALVLALLALELGPGDEVLTTPFSFFATVESILRVGATPRF
                     VDVERETLALDLAALEAAITPRTRAILAVHLYGYPGDLARLRELANRHRVTLIEDTAQ
                     AFGARFASRAAGTFGDLGCFSFQPSKPLGAMGDAGLVVTNDEELAARCRQLVVHGATR
                     KHHHVHVSGNYRLDTLQAVILEQKLASFPRYLAERAERARVYDAGLAGLPGLECPHAI
                     ENSEPSYALYTVRVLEAKRDALVRHLSSVGIETAVHYPEPLHRQPALLTRGLGAEVGT
                     LPVAERAAKEVLSLPLYPELPFDQLRSVVEAVREFCRSA"
     gene            complement(2004..2930)
                     /gene="tsf"
                     /locus_tag="VFQ61_00530"
     CDS             complement(2004..2930)
                     /gene="tsf"
                     /locus_tag="VFQ61_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET9952950.1"
                     /translation="MTQVNMQSVKELRDRTQAGLNDCRAALVEASGDMEKAVEIILKK
                     GLAKSAKRAGAIATEGLVVTRVSADGRTGVVVEVNIQTDFAARNEEFVRFANSVAEAA
                     LTAKGELGAEPFPGGGGTIEEVRAALVGKLGENINVRRSERISIDGPGRVHSYVHMGG
                     KIGVLLSARVQSDAVAQNPAFVQFLDDTAMQVAAMAPLYVSSREVPEDAKKKQSEIYD
                     AQLAEEGKPEKARPKIIEGKIAKWMKEVCLLDQPSVLQTEKSVEELAQELSKQLGSEV
                     QLGRFARFQCGEGIEKPKGDDFAAEVAKMAGG"
     gene            complement(2992..3879)
                     /gene="rpsB"
                     /locus_tag="VFQ61_00535"
     CDS             complement(2992..3879)
                     /gene="rpsB"
                     /locus_tag="VFQ61_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET9952951.1"
                     /translation="MTDLNPGVELPSQDPALSSDNDAGMSRKLRDPSQPLSVKELFEA
                     GVHFGHQTKRWNPKMRPYIYGTRSGIHIVDLDQTARLFKRAFDFIVDTVARGGSVLFV
                     GTKRQAQEIVQEEARRAGMYFVTNRWLGGTLTNFRTIKGGLDRLRSLERMKEDGTYEQ
                     LPKKEVVQLEKERARLEKYIGGLKGMGQLPQILFVIDPDQEAIAVSEARKLNIPIVAI
                     TDTNCDPDQVEYVIPGNDDAIRSVRLVTGAVADACVYGAARRRDFQQTREREGGRNEP
                     TVMYSGRGRGGGGGGDSSN"
     gene            complement(4083..>4426)
                     /locus_tag="VFQ61_00540"
     CDS             complement(4083..>4426)
                     /locus_tag="VFQ61_00540"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HET9952952.1"
                     /translation="GHGAPPKPITGSPDTLALHALTIDPKTGLPRGEKPPASTAAVLS
                     LVCGILLCLGPITGLSALIAGYLGRRAARERPQEVGGAGLALAGMLLGALNLFLSVMV
                     TAYVVYSMVLG"
BASE COUNT          761 a         1438 c         1382 g          845 t
ORIGIN      
        1 gttcgacgcg aagcggccaa agggttcacc gcgatcgttc acctggcggc catggccggc
       61 gtgcgcgctt cggtcgagga cccttggctt tattacgacg tcaatctgac cggcaccctg
      121 aacttgctcg atgcctcgcg cgaatacggc aaacccaact tcgtcttcgc gtccacctct
      181 tcggcttacg gcgctactcg gaccattccg ttcatcgaga cggacgcctc ggatcggccg
      241 ctctccccct accccgcgag caaacgtgcc gccgagctgc tcggctacac gtatcaccac
      301 ttgcacggcg cggactttac cgccctccgc ttcttcacgg tctatgggcc ccgcggcagg
      361 cccgacatga tggcgcacaa ggtcttcgac agcatccgga cgggcaaaga agtgccgctc
      421 tacaacggcg ggcaaatgta tcgcgactgg acgtacgtcg gcgacatcgt ctccggagta
      481 gtggcggcgg ccgatcgccg gcttggcttc gaggtcgtga acctcggccg cggcgaaccg
      541 gtgctgctca aagatttcgt gagcgccatc gagaagctgt gtggacgcac cgctcggctc
      601 accccggccc cgatgatcga cgcggacgtc agctacacgt tcgcggatat ttcgaaggcg
      661 cggcggctcc tcggatacga cccgaaagtc agcgtggatg aaggcgttcg gcacttccac
      721 gcttggtaca ccgagaacgt cggcgcattc gatactggct ctgcccccgc cacgccatga
      781 aagttcgaat gctggatctg gccgcgcagc acgaggcgct ggagccagag ttgagccgta
      841 gagttcgcga aatgatgcac tccgggaagt tcatcctcgg accggccgtc gcgagcttcg
      901 aacgagcggc cgcgggttac ctgggtgtgc agcacgcgat tggtgcatcg tccggctccg
      961 acgccctggt gttggcgctg ctggcgctcg agctaggccc cggtgacgag gtgttgacca
     1021 ctccgttctc cttctttgcg acggtggaga gcattctgcg cgttggagca acgcctcgtt
     1081 tcgtggacgt cgagcgagaa acgctggcgc tcgatcttgc ggctttggaa gccgcgatca
     1141 cgccccgcac gcgcgcgatt cttgccgttc acctgtatgg ctacccgggt gatctggctc
     1201 ggctacgaga actggcgaac agacaccgcg taacgctaat cgaggatacg gcccaagcct
     1261 tcggcgcgcg cttcgcgtcg cgtgctgccg gcactttcgg ggacctggga tgctttagct
     1321 ttcagccaag caagccgctc ggagccatgg gtgacgcagg cctggtggtg acgaatgacg
     1381 aagagctcgc cgcccgctgc cgccagttgg tcgttcatgg cgcgacccgc aagcatcacc
     1441 acgttcacgt gtccggcaac taccgtctgg atacgttgca ggcggtgatt ctcgagcaga
     1501 agctggcgtc ctttccgcgc tatctcgcgg agcgcgccga acgcgcgcga gtgtacgacg
     1561 ccggcctcgc cgggctgccc gggctcgagt gcccacacgc gatcgagaac tccgagccga
     1621 gctacgcgct ctataccgtg cgggtgctcg aagccaagcg ggacgctttg gtgcgtcact
     1681 tgtcgagcgt aggcatcgag accgcggttc actatccgga acccctgcat cggcaacctg
     1741 cgctgctgac gcgcgggctc ggagcagagg tcggcacgct cccggtggcg gagcgggcag
     1801 ccaaagaagt cctttcgttg ccgctctacc ccgagctccc gttcgatcag ctccgctccg
     1861 tggtcgaggc ggtgcgtgag ttctgccgat ccgcttgagc ggtccggttt ccgatccgcg
     1921 tcggcgcaca aaacgagaac ggggcgcctt gagacgcccc gttctgcttc gagaagctct
     1981 gctagtcaga gatcgagacc agatcagccg ccggccatct tcgcaacttc cgccgcgaag
     2041 tcgtcgccct tcggcttttc gatcccttcg ccgcactgga agcgggcgaa acggccgagc
     2101 tgcacttcgg agccgagctg tttgctgagc tcttgggcga gctcttccac gctcttctcg
     2161 gtttgaagaa cgctgggctg atcgagcaag cagacttcct tcatccactt ggcgatcttg
     2221 ccttcgatga tcttcgggcg cgccttctcg ggtttcccct cctcggcgag ctgggcatcg
     2281 tagatctccg attgcttctt cttggcgtct tcggggacct cgcgcgagga gacgtagagc
     2341 ggtgccatgg ccgcaacctg catcgccgta tcgtccagga actgcacgaa ggcggggttc
     2401 tgcgcgaccg cgtcgctttg cacgcgggcg gagagcagca cgccgatctt gccgcccatg
     2461 tgcacgtacg aatgaacccg accgggaccg tcgatgctga tgcgctccga gcgtcgcacg
     2521 ttgatgttct cgccgagctt gccgaccaga gccgcacgca cctcttcgat cgtcccgccg
     2581 ccgccgggga agggctcggc accgagctct cccttggccg tgagagcggc ctcggcgacc
     2641 gagttggcga agcgaacgaa ctcttcgttg cgcgctgcaa agtcggtctg aatgttgacc
     2701 tcgacgacga cgccggtgcg gccgtcggcc gaaacgcgcg tcacgacgag accctcggtc
     2761 gcgatggcgc ccgcgcgctt cgcgctcttg gcgagaccct tcttcaggat gatctcgacc
     2821 gccttctcca tatcgccaga ggcttccacg agcgccgcac ggcagtcgtt caacccggcc
     2881 tgggtgcggt ctcgcagttc cttgacggac tgcatgttca cttgagtcat ggtgtcactc
     2941 tttcgatgaa tgagtgtgtg ttcaacgaat gggaggtcaa ctagcggacg ctcaattgct
     3001 cgaatcgccg ccgccaccgc cgccgcggcc ccggcccgag tacatcacgg tcggctcgtt
     3061 ccggccgcct tcgcgttcgc gcgtctgctg gaaatcacgg cgacgggctg cgccgtaaac
     3121 gcatgcgtca gcgaccgcgc ccgtaaccaa acgcacggag cggatggcgt cgtcgttacc
     3181 cggaatcacg tactcgacct gatcgggatc gcagttcgtg tccgtgatgg caacgattgg
     3241 aatgttcagc ttgcgggctt cgctgaccgc gatcgcttcc tggtccggat cgatcacgaa
     3301 cagaatctgc ggcagctggc ccatgccctt cagaccgccg atgtacttct cgaggcgcgc
     3361 gcgctccttc tcgagctgga cgacctcttt cttcggcagt tgctcgtagg tgccgtcttc
     3421 cttcatgcgc tcgagagaac gcagccggtc gagaccaccc ttgatggtgc ggaagttcgt
     3481 gagggtgcca cccaaccagc ggttggtgac gaagtacatg cccgcgcgac gtgcttcctc
     3541 ttgcacgatt tcctgcgctt ggcgcttggt tccgacgaac agcaccgagc caccacgcgc
     3601 caccgtatcg acgatgaagt cgaaggctcg cttgaagagc cgcgccgtct ggtcgagatc
     3661 gacgatgtga atgccgctgc gcgtgccgta gatgtacgga cgcatcttcg gattccagcg
     3721 cttggtctga tgaccaaagt gcacgccggc ctcgaacagc tccttcacac tgaggggctg
     3781 ggacgggtca cgcagcttgc gcgacatgcc ggcatcgtta tcggaagaga gagcgggatc
     3841 ttgggagggc agttcgacgc ccggattcaa atcagtcacg ttagcgcact cctttcggtt
     3901 gtttcctccg ccccccatcc aagccaaccc ttcgaaactg cgaattgaac gacaattcag
     3961 ccaatctacg aagagcaccg aacttagacg ttgcgcgtgg gggcgtgcgt tttggggcgc
     4021 gaaagctagc ccacacctta ccggttggca agcccaagaa cccgacaggc ccacgagaag
     4081 cgtcagccca ggaccatcga atagacgacg taggcggtga ccatcacgga aagaaagaga
     4141 ttcagggcgc cgagcagcat tcccgccagc gcgagccccg ccccacctac ttcctgcggc
     4201 cgctcgcgcg ccgcgcgccg accgagatag cccgcgatca gggcggacag accggtgatt
     4261 gggcccaggc acagtaggat gccgcaaacc aaagacagga cggccgcggt gcttgcaggt
     4321 ggtttctcac cgcgtggcag cccggttttg gggtcgatgg tgagggcgtg caaggccagc
     4381 gtgtctggcg atcccgtgat tggtttcggc ggggcaccgt ggccat
//
LOCUS       DASTKU010000019         5792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1563907, whole genome shotgun sequence.
ACCESSION   DASTKU010000019 DASTKU010000000
VERSION     DASTKU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563907"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VFQ61_00545"
     CDS             complement(<1..1115)
                     /locus_tag="VFQ61_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952953.1"
                     /translation="MTLNRRGITALSLALALPALALAPLALQGCGAAGASSDAELNET
                     QSKEGQSSTQSWLQSGLATTVTEYHLPAYRDTEVLPSADIELWAALHRPATLVAGQRY
                     PLVVLLHGNHGTCRNVSTGAESNEYAETGVCRDGAEPIPNHRGYDYIASDLASHGYLV
                     LEVNANRGVHRQTITPGDPSHIRARGRLVLRHLEKLTAWHLGLEATPDTLGVSLQDHI
                     DFTQVGILGHSQGGEAVRLAYNEYRANGSPWPARIGTASAPLGIRGIFELAPTDGQAD
                     DLVLNADGTRWAVLLPGCDMDLTQPAGAYPYNRMLSIVEPNPNLKATYRVYGANHNFY
                     NTEWRQSDSHPVGCINQERLFDNPPLQRESGRYAVVS"
     gene            complement(1332..2123)
                     /locus_tag="VFQ61_00550"
     CDS             complement(1332..2123)
                     /locus_tag="VFQ61_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HET9952954.1"
                     /translation="MLPASDPLAEFRTVAPLGEHSLFAELLASVRFETVGKGRQGAVL
                     SQSDARDGTPLVRTTTRYSTPPQHFGLLHERLARLIQERASLSIAFNNALIECYTNAY
                     AAMGAHSDQTQDLAEDSIFSCYERPEAANPPRKLLLESKVASGGQFELSLAQNSVVVF
                     SVATNRRFKHRIVLDRAARSQENRWLGITFRTAKTFLRYREGCTYFADGTRLTQANEE
                     QKRELYALRRRENDEVDFTYPQLTYTLSDSDLMPPETAQPPTQAG"
     gene            complement(2107..3357)
                     /locus_tag="VFQ61_00555"
     CDS             complement(2107..3357)
                     /locus_tag="VFQ61_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952955.1"
                     /translation="MKATVRAEARIASAHNVLVAPTTLELKRSIPDFSGTVIRTNDIR
                     SVGLADLAGKSVYVCGDISQFNADALGAAERVFVIRELSHGYDEKHDGKSWTLVHLGR
                     VPVFVHGVGIYYRRFFELHSDYFHRICAEHTFQSLTESNKPEKAHRTGLYLTPVEQSG
                     EELHFRLLRCSTNLSGPTENFRETDREIVNALNREAAAIFEDPAPLNHVLAQIYNNSR
                     AGRGQKQAKAKISSHADKTKDMPTNGIMAFCTFYDQLDKLRPLTKDALDPDYFDYGYK
                     GVSGLTKLHFRLKAAVLESPECNLPPQFTLTLYPNSVFFMPLSTNRLYTHEIRASTLD
                     AELIPTRLGYVVRCSNAEAVHKDGHTFLKLNGKLVQLAPPSDEGMNELRRLYAEENKS
                     PGFIDYGENILFSMNGGDYSAPRV"
     gene            complement(3475..3654)
                     /locus_tag="VFQ61_00560"
     CDS             complement(3475..3654)
                     /locus_tag="VFQ61_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952956.1"
                     /translation="MGATLARLATVAIVATALFLRRGLLFIVTDHTLVPSATVDGSPE
                     ATHSEGPLVPSLEAR"
     gene            3653..4657
                     /locus_tag="VFQ61_00565"
     CDS             3653..4657
                     /locus_tag="VFQ61_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9952957.1"
                     /translation="MTVSRVINGGYASPEVRARVERAIKECGYAPSSTARSLRYGRKG
                     CIGVTVESVHGAWFMGILSGIEEELTRHHVGLMLTSLKPQDRYDSSSAAAWIEGRRVD
                     GLIMARFTKQEQPLLDAANRAGIPVTFICPDHSVEVGFTARCRNFDAGKLLGAHLLEL
                     GHRHIAFVGGPKESIDTSDRLRGLQEAVAAVRGASLRAEDITFQSSYRPQAGAAAGLS
                     FLKLPARKRPSAVVLGNDSMAIAFTSELLRAGLRVPGDVSVAGFDGIEEGERCFPTLT
                     TVAQPMHRLGVAACSALLERVQDPARDPGVTAEFPMALLQRESTGPFDGARRAAKKHA
                     "
     gene            4972..5331
                     /locus_tag="VFQ61_00570"
     CDS             4972..5331
                     /locus_tag="VFQ61_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HET9952958.1"
                     /translation="MSYIDGFIVPVPKAKLDEYRKFARLGSQIWREYGALDYREWVAE
                     DVKPGKLTSFPQSVQLGPDETVVFSWIVYKSRAHRDEVNEKVMNDPRMKIDPSTWPFD
                     GKRMIYGGFAPLEEGQE"
     gene            complement(5451..>5792)
                     /locus_tag="VFQ61_00575"
     CDS             complement(5451..>5792)
                     /locus_tag="VFQ61_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952959.1"
                     /translation="SLDGRPFTVGQPVKIDAHLSGANERDLVYFFYSAQADSSNVHWE
                     PLSDQPIQLTASGDIVVSASYTLPVGGVQAVRAALIQPLWEQKPVTPCISSFGPLEDH
                     DDIVFAVNPGG"
BASE COUNT         1084 a         1694 c         1861 g         1153 t
ORIGIN      
        1 aacgaaacga cagcgtagcg cccgctctcg cgctgcaggg gtggattgtc gaacagccgc
       61 tcctgattga tgcagcccac aggatgagag tcactctgtc gccattcggt gttgtagaag
      121 ttgtggttcg cgccatacac gcgataggtc gccttgaggt tggggttggg ctcgacgatg
      181 ctcaacatgc gattgtatgg ataggctcct gcaggctgag ttagatccat atcgcagcca
      241 ggcaacagca ccgcccagcg cgttccgtcg gcgttcagca ccagatcatc ggcttgacca
      301 tcggtgggtg cgagctcgaa gatccctcgg ataccgagcg gcgcagacgc agtgccaatt
      361 cgggccggcc aggggctgcc gttcgcgcga tactcgttgt aggcgaggcg tacggcctcg
      421 ccgccttgcg aatgacccaa gatcccgact tgggtgaagt cgatatgatc ttgcagactg
      481 acccccagtg tatctggcgt ggcttcgagc ccgaggtgcc aagccgtgag cttctcgaga
      541 tgcctcaaaa ccagccgccc acgggcgcgg atatgacttg ggtcacccgg tgtgatggtc
      601 tgccggtgta caccgcgatt cgcgttcacc tccagcacga ggtacccgtg ggaggcgaga
      661 tcggaagcga tgtagtcgta acctctgtga ttggggatcg gttcggcgcc atcacggcac
      721 acgcccgtct ccgcatactc gttggactcg gcgccagtgc tcacgttgcg acacgttccg
      781 tgattgccat gcagcagcac cacgagcggg tagcgctgcc ccgccactag tgtggccgga
      841 cggtgcagtg ccgcccagag ctcgatatct gcgctcggta gcacctccgt gtcacgatag
      901 gccggcagat gatactccgt cacggtcgtg gccagccctg attgaagcca actttgcgta
      961 ctcgactggc cttccttgga ctgggtttcg ttcaactcgg cgtcgctcga cgcgccggcc
     1021 gctccgcaac cttgtagagc caaaggggct aaagccaacg cgggtagagc caaagcgagc
     1081 gacaacgccg tgatgcctcg acgattcaat gtcatgttca atccctcggg tgagtccgtt
     1141 cgagagggtt ggagtgctgc gcacgtgaaa ccttcacctc gggggggata aaatctggcc
     1201 gagcgcaggt tcattcggcc ggctcttccc tcccgcgtgc gcttcgggca agccggccac
     1261 gccgattcaa atgtgaggcg caatgaccag cggcaccgca ggaatccgct cggcgtagat
     1321 gagcgtggtg ctcacccggc ttgtgttggg ggctgtgccg tctcgggtgg catcaggtcg
     1381 ctatcgctca aggtgtaagt gagctgcggg taggtgaagt cgacttcatc gttttctcgg
     1441 cgcctcagcg catagagctc acgcttttgt tcctcgttgg cctgcgtcag acgtgtaccg
     1501 tcggcgaagt aggtgcatcc ctcgcgatat cgcaggaacg tcttggcggt gcggaaggtg
     1561 atgcccaacc atcgattttc ttgcgagcgc gccgcccgat cgagaactat cctgtgcttg
     1621 aagcgccgat tggtggccac cgaaaacacc acgacgctgt tctgagcgag agagagctcg
     1681 aactggccac cgctcgcaac cttcgattcc aagagcagtt ttcttggtgg atttgcggcc
     1741 tcggggcgct cgtaacacga gaagatggag tcctccgcga gatcctgagt ttggtccgag
     1801 tgagctccca tcgcggcata cgcgttggtg tagcactcga tcagagcgtt gttgaacgca
     1861 atcgagagcg aggctcgctc ttgaatcagc cgtgccagcc tctcatgtag cagtccgaag
     1921 tgctgcggag gggtgctata tcgggtggtc gtgcgcacga gaggcgtgcc gtcccgcgca
     1981 tcgctctggc tcagaaccgc tccctgcctt ccttttccca cggtctcgaa gcgcaccgac
     2041 gcgagcagct ccgcgaacaa gctatgttcc ccgaggggcg cgacggtgcg aaactccgcg
     2101 agcgggtcag acgcggggag cactgtaatc tccgccgttc atgctaaaca ggatattctc
     2161 tccatagtcg atgaagccgg ggctcttgtt ctcttcggcg tacaggcgac gcagctcgtt
     2221 catgccctcg tcgctgggcg gcgccaattg caccagcttg ccattcagct tgaggaaggt
     2281 gtgaccgtcc ttgtggacgg cctccgcgtt cgagcagcgc acgacgtagc ccaggcgcgt
     2341 cgggatcaac tcggcgtcga gcgtggacgc tctgatttcg tgcgtataca gtcgattggt
     2401 ggacagcggc atgaagaaca ccgagttcgg ataaagagtc aacgtgaact gcgggggaag
     2461 attgcactcg ggcgactcga gaacggccgc cttcaatcga aagtgcagct tggtgaggcc
     2521 gctcactccc ttatagccat agtcgaagta atcagggtcc agagcatcct tggtgagcgg
     2581 ccgtagcttg tcgagctgat catagaacgt gcagaaggcc atgatgccat tggttggcat
     2641 gtctttggtt ttgtcagcgt gcgaggagat cttggccttg gcttgctttt gccccctgcc
     2701 ggcgcggcta ttgttgtaga tctgagcaag aacgtggttc agtggcgccg ggtcctcgaa
     2761 gatggcggcc gcctctcgat tgagcgcgtt cacgatctcg cgatcggtct cacgaaaatt
     2821 ctcggtgggg cccgagaggt tcgtggagca acgcagcagg cggaagtgca gttcttcgcc
     2881 ggactgttcc acgggggtca agtataggcc cgtgcgatgt gccttctcgg gtttgttgga
     2941 ctccgtgagg gactgaaacg tgtgctcagc gcaaatgcgg tggaagtagt cggagtggag
     3001 ctcgaagaat cgccggtaat aaatgccgac tccgtgcacg aagaccggaa cccggcccaa
     3061 gtgtacgagc gtccagctct tcccgtcgtg cttctcgtca tacccgtgcg aaagctcgcg
     3121 gatgacgaat actcgctccg ccgcaccgag ggcatcggca ttgaattgcg agatgtcacc
     3181 gcaaacgtac acgctcttcc cggccaagtc ggccagcccc accgaacgaa tgtcattcgt
     3241 acggatgaca gtcccggaaa agtccggaat cgagcgtttc agctccaggg tcgtgggcgc
     3301 gaccaggacg ttgtgggcgc tggcgatgcg ggcttccgct ctgacggtcg ctttcatggc
     3361 tgtacgatag ccgaatcagc ctcggatcat aaatctctgg tagcgctatc actcacacca
     3421 acgcctacaa cgcgccaact cggcttctgg tggctttgcg gctcgattca gctgtcatct
     3481 ggcttcgaga ctcggcacca gagggccctc cgagtgagtg gcctcagggc tgccgtctac
     3541 cgttgccgac gggactaggg tatggtccgt cacgatgaag agcagcccgc gacgcaaaaa
     3601 gagtgcagtc gctacgatcg cgaccgtggc caagcgagcc aacgtcgcgc ccatgacggt
     3661 ctcacgcgta atcaacgggg gttatgccag tccggaggtg cgggcccggg tagagcgcgc
     3721 catcaaggag tgtggctatg caccttcctc gacggcgcgc agcttgcgat atggccgcaa
     3781 gggctgcatc ggcgttacgg tcgagtcggt gcacggcgct tggttcatgg ggatattgag
     3841 cggcatcgaa gaggaactaa cgcggcacca cgtcggcctg atgctgacta gcctcaaacc
     3901 gcaagaccgt tacgattcga gcagcgcggc ggcgtggatc gagggacggc gcgtggatgg
     3961 cctcatcatg gcgcggttca cgaagcagga gcagccgctg ctcgatgccg ccaatcgggc
     4021 cggcattccc gtcaccttca tttgtccgga tcattcggtg gaggtgggtt tcactgcgcg
     4081 ttgccggaac ttcgacgccg gaaagctgct cggtgcgcac ctgctcgagc tcggtcatcg
     4141 gcacattgct ttcgtgggcg gccccaagga gtccatcgac acttccgacc gtctgcgtgg
     4201 cttacaagaa gccgtggccg cggtgcgcgg cgcgagtctt cgtgccgaag acatcacgtt
     4261 tcagagttca tatcggccgc aggcgggcgc ggcggcgggg ctctcatttc tcaagctgcc
     4321 cgctcgcaaa cgccccagcg cggtggtgtt gggcaacgac agcatggcca ttgcgttcac
     4381 cagcgaactc ttgcgtgcag ggctccgcgt tccgggagac gtttcggtgg cgggcttcga
     4441 tggcatcgaa gagggcgagc gctgcttccc gactctgacc acggtagcgc aaccgatgca
     4501 ccgcttgggc gtcgccgcct gctccgcact cctcgagcgc gtccaagatc cagcgcgaga
     4561 ccccggcgtc accgccgaat ttcccatggc cctcctgcag cgcgagagca ccggcccctt
     4621 cgacggagcg cggcgcgcgg ccaaaaagca cgcctaactg cgcggaaata ttcggctcca
     4681 caaccaagcc ttgccgccga tcaactccgt atggtaacga taccatctgg gtgcgagaag
     4741 cgcgtggttg tgaggggggg gtctccagag cggggcgatt gatcaccgat cgtcaatcaa
     4801 gaacagtcga ccagcaagct gtgcgttttg gcccagcgaa gcggcagttc acgagtcgat
     4861 tggacgatgt gttggacggg ggcgcggtgt aacctctgct gaccgctcaa attgcgccgg
     4921 cccatgcgtt aacggaccgg ctcgcggccg ctgagcggga aggagagctc gatgtcatat
     4981 atcgatggtt tcatagtgcc cgtccccaaa gcaaagctcg acgagtatcg gaagttcgcg
     5041 cgtttgggct cgcagatatg gcgagagtat ggcgcgctcg actaccggga gtgggtcgcc
     5101 gaggacgtga agccaggtaa actgacgtcc ttcccgcaaa gcgtgcaact gggccctgac
     5161 gagacagtcg tattctcttg gatcgtatac aagtctcgcg ctcaccggga cgaggtgaac
     5221 gagaaggtca tgaacgatcc gcgaatgaag atcgatcctt cgacttggcc attcgatggc
     5281 aagcgcatga tttacggtgg cttcgcgccg ctggaagagg gccaagagta gccagcggag
     5341 ccttcacctc tgtcgtgccg cgcgcgaggc tcgttccgct cgcgcgcggt gcgggatttc
     5401 gccctccatt ccgacgcgcg atcggtgaaa cggagcccgc ggacgagtgg tcagccgccg
     5461 gggttcaccg cgaatacgat gtcgtcgtga tcttcgagcg ggccgaagct gctgatgcac
     5521 ggggtcacgg gtttctgctc ccatagcggt tggatgagcg ccgctcgtac tgcttggacg
     5581 ccgcccacgg gcaacgtata gctggcggaa acgacgatat caccggacgc ggtgagttgt
     5641 atgggctgat ccgagagggg ctcccagtgc acgttcgagc tgtcggcttg cgctgagtag
     5701 aagaagtaga cgagatcgcg ttcgttcgcg ccagagaggt gagcgtcgat cttgacgggc
     5761 tgacccacgg taaatgggcg accatcgaga ct
//
LOCUS       DASTKU010000020         7880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_226392, whole genome shotgun sequence.
ACCESSION   DASTKU010000020 DASTKU010000000
VERSION     DASTKU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7880
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226392"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VFQ61_00580"
     CDS             <1..835
                     /locus_tag="VFQ61_00580"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET9952960.1"
                     /translation="AARELGVSTPALSQAVRKLEQRVGVALLTRTTRRVALTDAGRRL
                     ADEAGPALDRALEAVTRVASGGNAVGGRLRISIPPLAIPALVAPLIPRFARSYPDVEL
                     EFHVDNRRVDLIGEGFDAGVRFEEFVEKDMVQLRLTRPCRFVVVGSPEYLEKRGVPKK
                     PSDLHRHDCLEYRSPNTGRLYRWELERGHKRYRVAVRGKLVSNDERVLLELARAGMGL
                     CYAFEPQIAPQLEQGNLRVVLEPYAAAMPGLFLFFPNRSQVSPALRAFIDLVRTQLKE
                     L"
     gene            complement(963..1235)
                     /locus_tag="VFQ61_00585"
     CDS             complement(963..1235)
                     /locus_tag="VFQ61_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952961.1"
                     /translation="MTFPRPPKRCRRSADLGAARRHLELAREWPARTGEVEVALSVWE
                     LEARIALSAADRRAAMSAIDEGRALSAACGFGAFTERFDRLASTVS"
     gene            1543..2604
                     /locus_tag="VFQ61_00590"
     CDS             1543..2604
                     /locus_tag="VFQ61_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952962.1"
                     /translation="MTTGGTGTTSPQGGSAGSPPTGGASASPIGGSRSSGGIANQSTG
                     GSSIKIDECPPCVAPPHPSCRGLGTCGCGPYECPTPAGPAGEALEAADCRGPVQTDGE
                     HVYYTLGGMLYRITVGATYPERLAQEPPNPNALFVDASGIYTAHYGGVTWSDKNGGSI
                     RTIVGVSEAVRVAVAGGFAFYTVVNQGDVYRVALDAKTASGERIAEHSPHALAMAVDE
                     THVYWAAAKELLRVPVGGGTTEMLADNARGDAVALDSKYVYWTSDDGVQAMPKSGGAR
                     VPLVQVPVQGITTDGAHLYWTQGAQVSRTPSAGGPAEVVAGEGFEASAALAVDAKYVY
                     WLSCQGPSSAQATLRRIAK"
     gene            2826..3515
                     /locus_tag="VFQ61_00595"
     CDS             2826..3515
                     /locus_tag="VFQ61_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952963.1"
                     /translation="MFDADVRPVSVALLLGLAAASCGRTSHDDERPLAPSGGTSANTT
                     GASANTAGVTAAAGGAGGAATSIGGMTQEVIQAGAPTTLDPSTPSRACSEGDVPGAQC
                     EDPHCFGRRCGVRVDLVCRNGSWKNLNSSLAWDLTCPRGNEGVTDVGEIVTGACCGEE
                     LPKNDVFTEPPSCNLCPDAAPNDGDSCSLPNDCAPAIIDCFYECCCYGETIWAQCDGK
                     RWRVATNCSDK"
     gene            3609..4742
                     /locus_tag="VFQ61_00600"
     CDS             3609..4742
                     /locus_tag="VFQ61_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952964.1"
                     /translation="MPFKSPYKQKIDDGDLIYGIHAERKKLIEYLFKTSEVLMGTVRP
                     PPYLTVDVFDREPVRTVYKQHESLEKGSGKRSEQVLAWREFHQENAKHPKYKSYTEAT
                     EGGKKSEQGVEAVLSESVYNSIIRRKCKFGLYWTIKRGKHIHFCLDGLSMGAIAGKSF
                     KDDKGSGSDKLRAVTGSELRWIYRHRNVNAVQLGVQFWHNKAPAGPPWNEPEMQELIK
                     GKWVKWRESWASYKPKHEPSTLELDVDRSNATESALLGMTDEKATIVSCSLCGGGDIE
                     LHETSKGVLVCTRCVSPKNLPVSTVSLPLPRVSDSVDARLSCSKCKCTRASQTSNFFT
                     RWHECPSCGVVYCNICGSELGNEGLWFERGGFCECGGITELIY"
     gene            complement(4754..6589)
                     /locus_tag="VFQ61_00605"
     CDS             complement(4754..6589)
                     /locus_tag="VFQ61_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HET9952965.1"
                     /translation="MTQHASLSRFRRRGGGIRSTRARSRACVLLGACAIGYVACSNTE
                     PLETAARRQALSIAQSFQRGVSPDATYLGTADATLRQSAPGQNLGAAITCAVDGADRS
                     GLDTSCLFYWDVSSIPPGSIIAEARISIRITRGSSNVYDIYELLRPWSENQTTWNIAR
                     IGSPWNAPGASAADDRGAWLGTITGATNTTQTVTLNEAGLARVRAWVEDPSSNAGLIV
                     AHTTNSRELGMATGEHPTLAYRPTLSITYEPSSGGSSGGAPGAGGTSESGGTTSGSGG
                     ATAGTGGTTSGTGGSSGAGSLPGATEPELLVAFIGDQGANTNADAVLDLIKSEGAAAA
                     IHNGDFDYANNPTAWDDRITRILGANYPYFALIGNHDAAAWGGANGYAAKIAARHARV
                     PEMSCAGELGIKATCNFRGLYLIESCVGVTEFSTARCGKDSAEQVEFLHRALDLDSSL
                     WSICNWHKNQRDMQVGTKSDEVGWEAYRECMLGGALVATGHEHSFSRTVTLTDVGNRG
                     NSHGATGAFDLIQLAPGRNFVFTSGLGGTPVRPFDSTTHSEDTWWASYYTSDRWFKNG
                     SLQAGTGTFGAFFIRFHVGGDPKRADAYFKDVNGRVADEFTIRVP"
     gene            7202..>7880
                     /locus_tag="VFQ61_00610"
     CDS             7202..>7880
                     /locus_tag="VFQ61_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1587 domain-containing protein"
                     /protein_id="HET9952966.1"
                     /translation="MRCSQTKFAGLVTIWIASSVACSGTITEAPGSSSFTPQGSSTGA
                     QNPPVSTSGASASGAGAGNGEHTGSGGASWVSSAGEAGVSSGGGASLPGAGAPPVTGS
                     DPPYRALKCTTRAVGASPLRRLTHAEYDNAVRDLIGDRTQPASVFPSDTQVGLFDNTA
                     SVQSVPELLAEKYLDAAATLAEGITNVAGLVGCDYAAAGAGECVASFVNRFGRRAFRR
                     PLNGDEIA"
BASE COUNT         1482 a         2354 c         2578 g         1466 t
ORIGIN      
        1 cgcggctcgt gagctcggcg tctctacacc ggccctcagt caagccgttc gaaagctgga
       61 gcaacgagtc ggggtcgcgc tcctgactcg cacgacccgg cgcgtggcac tgaccgacgc
      121 tggacgccgt ctcgcggacg aggcggggcc cgcgctcgat cgagctttgg aggcagtgac
      181 tcgggtcgcg tcgggcggga acgcggtggg cgggcgattg cgcattagta tcccgccgct
      241 cgcgatcccg gcattggtgg cacctttgat cccacgcttc gcgcggtcgt atccagatgt
      301 ggagctcgag tttcatgtgg acaaccggcg cgtggatctg attggggagg gcttcgatgc
      361 cggggtgcgc ttcgaggagt tcgtcgagaa ggacatggtc caattgcgac tcacgagacc
      421 ttgccgtttc gtggtggtcg ggtcacccga gtacttggag aaacgaggcg tcccgaagaa
      481 accttcggac ctgcaccggc acgattgctt ggagtaccgc tcccccaata ccggtcgtct
      541 ctatcgctgg gagctcgaac gcggccacaa acgttatcga gttgccgtcc gtggcaagct
      601 cgtcagcaac gacgaacggg tgctcttgga gctcgcccgg gccggcatgg gcttgtgtta
      661 tgcgttcgaa ccgcaaatcg cgccgcagct cgagcagggc aacctacgag tcgtcctgga
      721 accctacgcc gcagcaatgc cagggctgtt tctgtttttt cccaatcgca gccaagtctc
      781 gccggcgtta cgagcgttca tcgacttggt ccgcacgcag ctgaaagagc tctgatgcag
      841 gctttacggc ttggggcgtg cgcggtgggc gcgacgcgga gttttcgagt agtttctgcg
      901 ctgtctcgaa cttcagtcga atctcgcgcc ctttgtctcg ccgggcccgc taatcctaga
      961 tgtcatgaga ccgtgctcgc gagtcggtcg aagcgctcgg tgaacgcgcc aaatccgcat
     1021 gcggcggaga gcgcgcggcc ttcgtcaatg gctgacatcg ccgcccgacg gtcggcagcg
     1081 gagagcgcga tgcgggcctc gagctcccag acgctcaacg cgacttcgac ttcgccggtg
     1141 cgcgcgggcc actcgcgagc gagttcgagg tgccggcgtg cggcaccgag atcggcgctc
     1201 ctgcggcagc gtttcggggg tctgggaaag gtcaccggga cggcagaagc tgcgcaggat
     1261 gatgccgatc ttgcattcag gaccgatggc acagcgcctg cttctgaggg gttggagcaa
     1321 atggacatgg cgctattggc acacgagggc cgactggggt gggcgtggct tttcctgctt
     1381 gggaccaatg gacttggagc gggatgctcg tcgggaaatg aggggacgag ctctggcacg
     1441 ggtggcacaa tcgccgccag cggagcctcg gctggtggcg cagccggtat gaccgggagt
     1501 cgcgccgacg gaggtaacgg ggccagggag ggagggtccc tggtgacgac aggaggaacc
     1561 ggtacgacga gcccgcaagg tggcagcgcc ggttcgccgc cgaccggagg cgccagcgcc
     1621 tctccgatag ggggctcgag atcgagtgga ggcatcgcga accagagcac cggaggcagc
     1681 tccatcaaga tcgacgagtg tccgccgtgc gtggccccac cgcacccaag ctgccgcggc
     1741 ctcggcacgt gcgggtgcgg accctacgag tgcccaaccc cggcagggcc agcaggcgag
     1801 gcgctggagg cagccgattg cagaggcccg gtccaaaccg acggcgagca cgtgtattac
     1861 acgttagggg gcatgctcta tcgcatcacc gttggcgcga cgtatccgga gcgcctcgcg
     1921 caagaacccc cgaatccaaa cgcgctcttc gtggacgcat ccggcatcta tacggcgcac
     1981 tacggcggcg tcacttggag tgacaagaac gggggtagta tccgcacaat cgtgggcgtg
     2041 agtgaagcag tgagggtggc cgtcgccggc ggctttgcat tctataccgt cgtcaaccag
     2101 ggcgacgtgt atcgcgtcgc cttggatgcg aagaccgcga gtggcgagcg catcgccgaa
     2161 cactccccgc atgccctcgc gatggcggtt gacgaaaccc acgtctactg ggcagccgcc
     2221 aaggagcttt tacgtgtacc cgtgggtggc ggcacgaccg agatgttggc cgataatgct
     2281 cgcggcgatg ccgtggctct cgacagcaag tatgtatact ggaccagcga tgacggcgtc
     2341 caagccatgc cgaagagcgg tggcgcacgg gtgcccttgg tccaggtgcc agtgcagggc
     2401 atcacgaccg atggcgcgca cctctattgg acccagggcg cacaggtcag tcggaccccg
     2461 agcgcaggcg gccccgccga agtcgtagcc ggagagggtt tcgaggcgtc tgccgctctc
     2521 gccgtcgatg cgaaatacgt ctactggttg agttgccagg ggccctcatc tgcccaagca
     2581 acgctccggc gtatcgctaa gtgaaggcgc accaggcggg cagcatcagc ttggcgcgga
     2641 taggaatttc gaacgccgaa tggcgcacag gctgactcac tgcggcgctc gtatcttcgc
     2701 cggctcatcg gaccccgttc gacccgcccc ctcgcattct agacaagcac cccgagggag
     2761 cctaccatgt cgagcgactg acaaactagg gtatcgccgg aagctgggtt gaaaggacgg
     2821 tgtgtatgtt tgacgctgat gttcgaccgg tctcggttgc tctgctgctc ggactcgcgg
     2881 ctgcaagctg tgggcgtacc tcccacgatg acgagcggcc cctagccccg tccggcggca
     2941 cctctgcgaa cacgactggt gcaagcgcga acacggctgg tgtcaccgcg gccgcgggtg
     3001 gcgcgggcgg ggctgcaacg tccataggcg gaatgacgca ggaggtaatc caagcaggcg
     3061 ccccaaccac cctcgatccg tccactccgt cgcgagcctg cagcgaagga gatgtgcccg
     3121 gagctcagtg cgaagatcct cattgcttcg gcaggcgctg cggcgtccgc gtggatctcg
     3181 tttgccgcaa cgggtcgtgg aagaacctca attcatccct ggcctgggac ttgacctgtc
     3241 ctcgaggcaa cgaaggtgtc actgacgttg gcgagatcgt gaccggcgcg tgttgcggcg
     3301 aggagctccc gaagaatgac gtctttacgg agcccccgag ctgcaacctc tgtccggacg
     3361 cagcgcccaa tgacggggat tcgtgctcgc tacccaacga ttgtgcgccc gcgatcatcg
     3421 attgtttcta tgagtgttgt tgctacggag aaacgatctg ggcgcagtgt gatgggaaga
     3481 ggtggcgggt cgcgacgaac tgctcggaca aataggcggc gtgggcgcgc cccacactca
     3541 ccgccggatc cgtacacgtt ccgtccaaaa tattgacgtt ctgagaggcg gcacgggatg
     3601 atccaagtat gccattcaag tctccgtaca agcagaagat cgacgatggc gacctcatct
     3661 acggcatcca tgccgagcga aagaaactca tcgaatatct attcaagacg tccgaagtgc
     3721 taatgggcac ggtgcgcccg ccgccgtacc tcaccgtgga cgtcttcgat cgtgagccgg
     3781 taaggacggt gtataaacag cacgagtcgc tcgagaaagg tagtggcaag agatccgaac
     3841 aggtgctggc ctggcgagag tttcaccagg agaatgccaa gcaccccaaa tacaagagtt
     3901 acaccgaggc aaccgagggt ggaaaaaaga gcgaacaggg agtggaggcg gtgctctccg
     3961 agtccgtgta caactccatc attcgacgga aatgcaaatt cgggctctac tggacgatca
     4021 agcgcgggaa acatatccac ttctgcctcg atgggctcag catgggggcg atcgcgggca
     4081 agtcgttcaa ggacgataaa ggcagtggta gcgataagct gcgggcggtg actggctccg
     4141 aattgcgctg gatttaccgg catagaaacg tgaatgccgt gcaactgggg gtccaattct
     4201 ggcataacaa agcccctgca ggtccccctt ggaacgagcc cgagatgcag gaactcatca
     4261 aggggaaatg ggtgaaatgg cgtgaaagct gggcgtccta caaacccaag cacgagccat
     4321 cgaccctaga gttggatgtg gaccggtcga acgcgaccga aagcgccctc ctgggaatga
     4381 cggacgaaaa ggccaccatc gtgagttgca gtctctgcgg agggggcgat atcgagctcc
     4441 acgaaacgag caagggggtt ctggtttgca ctcgatgcgt ttcccctaaa aacctccctg
     4501 tgtccactgt ctctctaccg ctcccgcgtg tgagcgacag cgtcgacgct cgcctatctt
     4561 gctccaagtg caaatgtacg cgagcctctc agacgtcgaa cttcttcact cgttggcacg
     4621 agtgtccaag ctgtggagtc gtgtattgta atatctgcgg cagcgaactc ggcaatgaag
     4681 gcttgtggtt cgagcggggt ggtttttgtg agtgcggagg gatcaccgag ttgatctatt
     4741 gatcgagcga agcctaaggc actcgtatcg taaattcgtc ggcgactcga ccgttcacgt
     4801 ctttgaagta ggcgtccgca cgcttcgggt cgcctccaac gtggaagcgg atgaagaatg
     4861 cgccaaaggt gccggtgccc gcttgtagcg agccattctt gaaccagcga tcgctcgtgt
     4921 aataggacgc ccaccacgtg tcctcgctgt gggtcgtgct atcgaagggc cgaacggggg
     4981 tgccgccgag gccggaggtg aatacgaagt ttcgaccagg ggcgagttgg atgaggtcga
     5041 aggccccggt ggctccatgg gagtttccgc gattaccaac gtccgttagc gtcacggtgc
     5101 gagagaagga gtgctcgtgc ccggtcgcca cgagcgcgcc acccagcata cattcacgat
     5161 atgcctccca accgacttcg tcgctcttgg taccaacctg catatcacgc tggttcttgt
     5221 gccagttgca aatggaccag agggatgaat cgaggtccag agcgcggtga agaaactcga
     5281 cctgctcggc ggaatccttc ccgcagcgtg cagtgctaaa ttcggtgacg cccacgcagg
     5341 attcgattag gtagaggcca cggaagttgc aggtggcttt gatgccgagt tcgccggcgc
     5401 agctcatctc gggaactcgc gcgtggcgcg cggcgatttt cgcggcatag ccgttggcgc
     5461 ccccccaggc cgcagcgtca tgattgccaa tcagggcgaa gtacgggtag ttggctccca
     5521 ggatccgcgt gatacggtcg tcccaagcgg tcggattgtt ggcataatcg aaatcgccgt
     5581 tgtggatcgc ggcggcggcg ccttcgctct tgatcaaatc gagtactgcg tctgcgttgg
     5641 tgttcgctcc ctggtctcca atgaaggcga ccaggagttc cggctcggtc gcccccggaa
     5701 ggctcccggc gccagaactg ccgccggtgc cagacgttgt tccgccggta ccggccgtgg
     5761 ctccgccgct tcccgacgtt gttccgccac tttcactcgt gcctcccgct ccgggcgctc
     5821 cgccgctgct tcccccactg gacggttcat aggtgataga gagtgttggg cgataggcta
     5881 gcgtcgggtg ttcaccggtc gccataccga gttctctgga gttggtcgta tgggccacga
     5941 tgaggccggc attgctactc gggtcttcga cccaagcccg cacccgggcg agcccggcct
     6001 cattcagagt tacggtttgc gtggtgttgg ttgctcccgt gatggtgcct agccacgcac
     6061 ctcggtcgtc agccgcagaa gcgccgggcg cgttccaggg gctgccgatg cgagcgatgt
     6121 tccaggtggt ttggttttcg ctccacggcc gaagcaactc gtagatgtcg tatacattgc
     6181 tgctgcctct ggtgatccga attgagatgc gcgcctcggc aatgatgctg cctgggggga
     6241 tcgaactgac atcccaatag aagaggcaag aagtatcgag cccgctacga tctgcgccgt
     6301 cgacagcaca tgttattgcg gcgcccaggt tctgccccgg cgcggactgc cgcaaggtag
     6361 cgtcagccgt accgaggtac gtagcgtcgg gcgagacgcc ccgctgaaag ctttgcgcga
     6421 tcgagagcgc ttggcggcgc gctgccgttt ccagcggctc tgtgttgctg cacgcgacat
     6481 acccaatcgc gcatgccccg agcagcacgc aggcgcgcga gcgcgcccgt gtactgcgga
     6541 tgcccccgcc ccgccgtcga aacctggaaa gagaagcgtg ctgagtcatt ctatctcctg
     6601 tgtctaagtc cagtcgccgc gaatcccttc ggcggagcgc gaactcgcca ggcagctcac
     6661 tcgctatcgc ggctgccccg aatgccggga ttcgtgccct cgcctcactc actattattg
     6721 ccgcacgagc gagcgattca tgtagatgtt ttgtcgcagc ggtcccgaca acgaaaaatt
     6781 actagagccc gcgggagcac accgcgaact ctgccgtacg gcggactctg ccgtacggag
     6841 taaacgtgcc cccgcggggg gcacttgaaa ctcagagcac tgcggctcca gtatcgccag
     6901 cgcgcttgcg gatgaaagac gcgcacccta gtgccggcgt cacacccacc gagcacgagc
     6961 gcttgggcgt tggtgcccgc gcacacgtgg ctcgtcatcg cgagtgctcg aagcatcgcc
     7021 tcaagttgag gcaactaatc gagctctcga gccacccgac ttcgctcaat ccaggcacat
     7081 cccatcaatc gagagtgcaa agctggatgt cttttcggtt cggcctcacg tcatcgagct
     7141 cgcgaaatct cgtgagctga ttcgatgagt cgaggggacg tactacccca aggacggttg
     7201 aatgcgctgt tcgcaaacga agtttgctgg gctggttacg atttggatag cgagttccgt
     7261 tgcttgttcc ggaaccatta cggaggcgcc gggctcgagc agtttcactc cccagggtag
     7321 ttccacgggg gcacagaacc cacctgtttc tacttcaggc gcgtcggcat cgggtgcggg
     7381 ggcaggcaac ggcgagcaca cgggctcggg gggcgcctcc tgggtcagca gcgccggcga
     7441 ggccggggtc tcgagcggtg gcggagctag tctgccgggt gcaggggctc cgcccgtcac
     7501 gggcagcgac ccgccatatc gagccctgaa gtgcacgact cgcgcggtag gggcgtcccc
     7561 tctgcggcgg ctcacgcacg cggaatacga caacgccgtg cgcgatttga ttggcgatcg
     7621 cacccaaccg gcgtccgtct tcccgagtga tacgcaagta ggacttttcg acaatacggc
     7681 ttcggtgcag agcgtgccgg agttgctggc ggagaaatac ctggatgcgg ccgccacgct
     7741 cgccgaggga atcacgaatg tcgcgggatt ggtaggctgc gactatgctg cagccggcgc
     7801 aggcgagtgt gttgcgagct tcgtgaaccg cttcggaagg cgcgcattcc ggcgcccatt
     7861 gaacggtgac gagatcgcca
//
LOCUS       DASTKU010000021         5733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_449456, whole genome shotgun sequence.
ACCESSION   DASTKU010000021 DASTKU010000000
VERSION     DASTKU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449456"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VFQ61_00615"
     CDS             complement(<1..526)
                     /locus_tag="VFQ61_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952967.1"
                     /translation="MTETAQRTRSPLAWVPSLYLAMGIPFSLVIWVAGTMFKDLGHSD
                     TSIALATGSVGIAWSLKPLWASFLDMYRTKKFWVLSMEFVIAILLGLIAAALPLPSYF
                     QVIIGILWVLAFSSSTQDICADGVYITTLKEEEQAKWIGWQGAAWNTGRIFATALVVY
                     VAGKLQEGGQSPKAA"
     gene            complement(722..1987)
                     /gene="glgA"
                     /locus_tag="VFQ61_00620"
     CDS             complement(722..1987)
                     /gene="glgA"
                     /locus_tag="VFQ61_00620"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HET9952968.1"
                     /translation="MIRLESQQSSGQSLALSPAQLSVALLTREYPPENYGGAGVHVEY
                     LARELKRRIELGVYCFGAPRSAEEVKGTFAPWDLLAGSAPELQALRTLSVNLLMTKAI
                     QGCSLSHSHTWYANFAGHLAKLSYGMPHVMTSHSLEPLRPWKAEQLRGGYAISSWCES
                     TAIHAADAVVAVSQGMKADILRAYPGVDPSRVHVIYNGVDPNEFVPKEGRDVLARYGI
                     DPAQPYAVFVGRVTRQKGIVHLLRAGRYLKPGSQLVLCAGEADTPELGQEVRGLVDEL
                     KSVRGGVIWIEKMLSRPELSQILSNATVFCCPSVYEPFGIVNLEAMACGAAVVASKVG
                     GIPEIVVDGQTGYLVPFEADGTARAEPKHPEVFAQALAEKLDLLLADPTRAREFGAAG
                     RRRVVETFSWSSIAEQTIALYQDVLARTR"
     gene            complement(2045..3298)
                     /gene="glgC"
                     /locus_tag="VFQ61_00625"
     CDS             complement(2045..3298)
                     /gene="glgC"
                     /locus_tag="VFQ61_00625"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET9952969.1"
                     /translation="MAKKRPHVLGMVLAGGEGNRLWPLTRDRAKPAVPFGGCYRLVDF
                     ALSNLVNGRYLRTAVLTQYKSHSLDRHIAQTWRMSPLLNNYVMSVPAQMRLGRRWFEG
                     SADAIYQNLNLITDENPEYICVFGADHIYRMDPEQMVDQHIESGAGVTVAAIRVPRNE
                     ASRFGVLVPGANGRIQKFKEKPDPKEISGLPNDPEHVFASMGNYVFTTKTLVEAVRAD
                     AENKDSAHDMGGNIITGLVESGLASVYDFAKNVVPGATERDAGYWRDVGTLDSYYEAQ
                     MDLISVHPVFNLYNQEWPIHTMQISLPPAKFVFDNDGRRGMAVDSIVSAGAIVSGGLV
                     RGSILSPDVLVHSYSEVEDSVLMHGVEIGRRAIVRKAILDKGVKVPAGAKIGVDPESD
                     RRRGFTISPNGVVVIGKGDPVPLDP"
     gene            complement(4326..5243)
                     /gene="atpG"
                     /locus_tag="VFQ61_00630"
     CDS             complement(4326..5243)
                     /gene="atpG"
                     /locus_tag="VFQ61_00630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET9952970.1"
                     /translation="MANLKHIRKRIVSVKSTQKITRAMKMVAAAQLNRAQQRIIAVRP
                     YAVKTAQVLSEVVVTSAETQDGEHAELMHPLLARRPEKRALILVVTGDRGLCGAFNSN
                     VLRLADRVWREREAEGQKVMFGVVGRKGRDYFRRRGAPVFNNFHNIWERLDLEQARGI
                     ARSLLTPFARGEVDAIYLVYNEFKSAVSQRPVMELLFPLPVPQSTTERAEAKEEWARH
                     REFLFEPDQSALLERLVPMYVEISILRALLESRASELGAQMTAMDAATKNASEMIAKL
                     TLDYNRARQAAITTELMEIIGGAEALRNG"
     gene            complement(5317..>5733)
                     /locus_tag="VFQ61_00635"
     CDS             complement(5317..>5733)
                     /locus_tag="VFQ61_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET9952971.1"
                     /translation="QFREMAAFAQFASDLDAATRAQLERGRRLTELLKQGQFVPLPLE
                     RQVVTIFAGTNGYTDKLPVESLKAYEEELYRFLEEKHPDIFNDIREKAELTDDIRGRL
                     DKALKAFGKKFVAGTSADGKGAKRAAAETEAEADAE"
BASE COUNT         1103 a         1788 c         1737 g         1105 t
ORIGIN      
        1 aagccgcctt cggcgattgg ccaccttcct gcagcttgcc agcgacgtag acgaccaagg
       61 cggtcgcaaa gatgcgcccg gtgttccagg ccgctccttg ccaaccgatc catttcgctt
      121 gctcctcttc cttgagcgtc gtgatgtaca cgccgtcagc gcagatatcc tgtgtggagg
      181 aggagaacgc aagtacccag aggattccga tgatgacctg gaaatacgag ggcaacggga
      241 gcgcggccgc gatcaacccg agcaagatcg cgatgacgaa ctccatgctc agcacccaaa
      301 acttcttggt gcgatacatg tcgaggaacg aggcccacag cggcttcagg gaccaggcga
      361 taccgacact gcccgtcgcg agcgcgatcg aagtgtccga gtgccccaag tctttgaaca
      421 tcgtgccggc gacccagatc acgagggaga atggaatccc catcgcgaga tacaagcttg
      481 gcacccaggc gagcggactg cgcgtccgct gagcagtctc agtcatgctg ctggtaatat
      541 gcaaagtccg gccggaaacc aagggaggag tgacggattg cgcttcaacc gtcacccctg
      601 actggcgcca cgatgctcgc gttgttgggc gacgctagct ttgctgggca acgctagctt
      661 tgccgggcga accgcggggg ctcgcgttcc gtcgcgcagg aacgctcacg ggccgagacg
      721 attagcgcgt gcgagccaaa acgtcttggt agagcgcaat cgtctgctcg gcgatgctgg
      781 accagctgaa agtctcgacg acccgacgcc gccctgccgc cccgaactct cgagcacggg
      841 tcggatcggc cagcaataag tccagtttct cggcgagagc ctgggcaaac acctcagggt
      901 gtttgggctc ggcgcgcgcg gtaccatccg cttcgaaggg caccaggtag cccgtttgcc
      961 cgtccaccac gatttccgga atgcctccga ctttcgaggc caccaccgcg gcgccgcaag
     1021 ccatcgcctc gaggttcacg ataccgaaag gttcgtacac ggatggacag caaaatactg
     1081 tcgcgttcga gagaatttgt gagagttctg gacgggaaag catcttttca atccagatca
     1141 caccccctct gacgctcttg agttcgtcca ccaagccgcg aacttcttgt cccagttcgg
     1201 gcgtgtccgc ctcccctgcg cacaagacga gctgagagcc tggcttcaag tagcggccgg
     1261 cacgaagcaa atgaacgatg cctttctggc gcgtcactcg cccaacgaac acggcataag
     1321 gttgcgccgg gtcgatgcca taacgggcaa gaacgtcgcg tccctctttt ggaacgaact
     1381 cgttcggatc cactccgttg tagatcacgt gcacgcgcga gggatccacg cccggataag
     1441 cccgcaggat gtcggccttc atgccttgcg agaccgcgac cactgcgtct gccgcatgga
     1501 tggccgtgct ctcgcaccat gacgagatgg cataaccgcc ccgcagctgc tcagccttcc
     1561 agggccgcaa cggctcgagg ctgtgcgagg tcatgacgtg cggcatcccg taggacagct
     1621 tggcgaggtg acctgcaaag ttcgcgtacc aggtgtggct atgagaaagc gagcagccct
     1681 gaatcgcttt ggtcatgagt aggttgaccg agagcgtacg cagggcctgc aactcaggtg
     1741 cggaaccggc caggaggtcc cagggggcga acgtaccctt tacctcttct gcactgcgag
     1801 gggcaccaaa gcagtacacc cctagctcga tgcggcgttt cagctcgcgc gccaaatact
     1861 cgacgtgaac gcccgcgccc ccgtagttct ctggtggata ttcgcgggtg aggagcgcga
     1921 ctgagagctg agctggggaa agggctaggg actggcctga agactgctga ctttccaaac
     1981 gaatcacgcc ttgctgtggg gccgccctag cctcgctggc actagcctgg gaaccagaac
     2041 gacgtcatgg gtctagcgga accggatcgc ctttgccaat gaccacgacc ccgtttggcg
     2101 aaatggtgaa cccgcgtctc cgatcgcttt caggatctac gccaatcttg gcgccggcgg
     2161 gcaccttcac gcccttgtcc aagattgcct tgcgcacgat ggcacggcgg ccgatctcga
     2221 cgccatgcat caagacgctg tcttccacct ccgagtaaga atgcaccaat acatcgggtg
     2281 aaagaatgct gccgcgcacg aggccacccg agacgatggc gcccgcgctg acgatggagt
     2341 cgactgccat gccgcgccgc ccgtcgttgt cgaacacgaa cttggcggga ggcagcgaaa
     2401 tctgcatcgt gtgaatcggc cactcctgat tgtagagatt gaatacggga tggacgctaa
     2461 tcaggtccat ctgcgcttcg taataactgt ccaaggtgcc cacgtctcgc caatatcccg
     2521 cgtcgcgctc ggttgcgccg ggaactacgt tcttcgcgaa gtcgtagacg gaggccaagc
     2581 cggattcgac taggccagtg atgatgttgc cgcccatgtc atgagcgctg tccttgttct
     2641 cggcgtcggc gcgcacggct tcgaccaacg tcttcgtcgt aaatacgtag ttgcccatcg
     2701 aggcaaagac gtgttccgga tcattgggta agccgctgat ctccttggga tcgggcttct
     2761 ccttgaactt ctggatgcgg ccgttggcgc ccggaaccag cacgccgaag cgcgaagctt
     2821 cgttgcgcgg cacacgaatc gcggccaccg tcacgccggc gccggactcg atgtgctgat
     2881 cgaccatctg ttccgggtcc atccgataaa tgtgatctgc cccgaacacg cagatgtact
     2941 cggggttttc atcggtgatc aggttgaggt tttggtagat cgcgtccgcg gagccctcga
     3001 accaacgccg gcccaaacgc atctgggcgg gcacactcat aacgtagtta ttcaacaagg
     3061 gcgacatgcg ccaggtctgg gcgatgtgtc gatcgaggct gtggctcttg tattgagtga
     3121 gcacggccgt acggagatag cgaccgttga ctagattgct gagtgcgaaa tccacgagcc
     3181 gatagcagcc cccgaagggc accgcgggct tcgcgcgatc tcgcgtcagc ggccacaggc
     3241 gattgccctc tcctccagcg agcaccatgc cgagcacgtg cggacgtttc tttgccatgc
     3301 tccgaaatct tgcaccagga cggcccggag gtgaaccccc cagacctgct aaaccccact
     3361 ccatcgccgg cgaacggacg atcctcattt tcaggccaga tccccctcgc taacacgcac
     3421 acttcgcgac tgcacccgcc agagctcgcc actcgattag caaacaacgc cgtaaaagca
     3481 cgccgcgttg gaagccgccg gtagaaccca gccccgacgt cgacggtcta aaaggcgagt
     3541 tcgaaacgcg aggtagtccc gccggtcgcc ccggcgccga actacagcca tttcgtgccg
     3601 ccatcccgga tttttcggtt tattcaatca tggtttccgg cgaattgcga aaggggcgaa
     3661 atgtcgccga agcttcgaac gcagcgctct gcgatttctc ggacttcgcg ttagaacctc
     3721 gagcacgacg gcttcaagat ggccgtaaga cgcgagctat cggctgccaa cgactagttg
     3781 ccactgggat aggttcttag cctggatttc gcgacggcga ccgacagaag tcggcaaggc
     3841 ggcggcgagc acgacttcaa ttggcttcag cacgcgcggg gcacgctccc ggttcgccag
     3901 catcaaggct agctttggtg tcagcgcgac gccggaatcg acatcatgag tttcatcgaa
     3961 aacgaggtgc tcgccgacga tcccgtggca ccaagccgcg ctggactgcc aaatggaact
     4021 gcgcccgaga acgctggcgt ggctgaatgc caatcaatcg ccccaactcc atgtttcgca
     4081 catcgacccg atcgggagct gaatagtcca aacttgatcc aatcttcaaa acacgacggc
     4141 cgagctctcg tgtcgagaac tcggccgctg tggtttctag ggaagtttgg actggcggtt
     4201 atgctggtac atttggcgta acgggctagc cgttacgttg gttgcattgg cgtaaggctt
     4261 agccgttacg ctggttgcat tggcgtaacg ggctagccgt tacgctggtt tcattggcgt
     4321 aacggctagc cgttacgcag tgcctcggcg ccgccgatga tttccatgag ctccgtcgta
     4381 atcgcagctt ggcgggctcg gttgtagtcg agcgttagct tggcgatcat ttcggatgcg
     4441 ttcttggtgg ccgcgtccat ggccgtcatt tgcgcgccga gctccgacgc ccgcgactcg
     4501 agcagggccc gcaagatgga tatctcgacg tacatcggca cgagccgctc gagcaaagcc
     4561 gattgatccg gctcgaacaa gaactcgcga tgacgcgccc actcttcctt ggcctcggcg
     4621 cgctcggtgg tcgactgcgg cacgggcagc gggaacaaca actccatgac ggggcgctgc
     4681 gagacggcgc tcttgaactc gttgtacacg aggtagatgg cgtccacttc gccgcgcgcg
     4741 aagggcgtga gcaacgagcg agcgatgccg cgcgcctgct ccaaatccaa ccgctcccag
     4801 atgttgtgga agttgttgaa gaccggggcg ccacggcgac ggaagtaatc gcggcctttg
     4861 cgcccgacta ctccaaacat caccttctgc ccttcggctt cgcgctcacg ccagacgcga
     4921 tccgccaggc gcagcacgtt cgagttgaac gcgccgcaca ggccgcggtc gcccgtgacg
     4981 accaggatca aggcccgctt ctcgggacgg cgcgcgagaa gcgggtgcat gagttcggcg
     5041 tgctcgccgt cctgcgtctc cgcggaggtg accacgacct ccgagagcac ctgcgccgtc
     5101 ttcacggcgt aggggcgcac ggcgataatc cgctgctgag ctcggttgag ctgagctgcg
     5161 gccaccatct tcatcgcgcg cgtgatcttc tgcgtgctct tcacgctcac gatgcgcttg
     5221 cggatgtgct tgaggttggc caccgctagt tcctcttctt cgtttcctgt tcagacgctg
     5281 ccaagtattc gggccgattg cgtgcgcgcg agtggctcac tcggcgtcgg cttctgcctc
     5341 ggtctcggcc gcagcgcgct tggcgccctt gccgtccgcg ctggttccgg cgacgaactt
     5401 cttgccgaag gccttgagcg ccttgtccaa gcgaccacgg atgtcgtccg tgagctcggc
     5461 cttttcgcgg atgtcgttga agatgtccgg gtgcttctcc tcgaggaagc gatacagctc
     5521 ttcttcgtag gccttgagcg actcgaccgg cagcttgtcg gtgtaaccgt tggtgccggc
     5581 gaagatcgtg accacctggc gctcgagcgg cagcggaacg aactggccct gcttcagcag
     5641 ctcggtcagg cggcggccgc gctcgagctg ggcccgagta gcggcgtcga gatcgctcgc
     5701 gaactgagcg aaggctgcca tctcgcgaaa ctg
//
LOCUS       DASTKU010000022         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1341824, whole genome shotgun sequence.
ACCESSION   DASTKU010000022 DASTKU010000000
VERSION     DASTKU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341824"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFQ61_00640"
     CDS             complement(<1..635)
                     /locus_tag="VFQ61_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HET9952972.1"
                     /translation="MSGASSGSGASGGTSTLPNGANSSGGDTGLPVAGGAGVNSGSPG
                     NGGALGNGGALGNGGALGNGGAPGSSGGLGNVGGTNAGGRSSTGGAENTSDAGAGGAG
                     ESPSCPPAAALTGGRRYCSNSKGNASGNYSYELWAEGSGSGCMTVYGKGAAFGANWNK
                     VEDLLARVGLQFDRSKTHSQLGTISAEFAETKTGGDGLVYVGVYGWTVNPLR"
     gene            1630..>2733
                     /locus_tag="VFQ61_00645"
     CDS             1630..>2733
                     /locus_tag="VFQ61_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9952973.1"
                     /translation="MKPTAFLSSRVERLDKIHDGKRSVVYRARLRESKRPVIAKVLRG
                     PYPSPEQQSRFRREYEVGRALHGPHIIETIALETGEDTLAILMEDCGAESLAGRGPLE
                     LGEFLPLARDIVLGLAHIHACAVAHRDINPSNIVWNRGRKLVKIIDFGISRAVSRESL
                     ELDPNVDPHLERGFEGTLAYISPEQTGRINRSVDHRTDLYSLGVTFYELLTGQRPFAG
                     EDAMELIHAQIARVPVAPHERRASVPRPLSDLVMKLLEKNPADRYQSASGVNADLEEC
                     SARHARTGHIAGFELARRDTAARLQLPQGLYGRDHEFASLRNALARSAAGRSQIALVS
                     GYSGIGKTRLVRELRGPTAALGGYFVSGKFEQFR"
BASE COUNT          520 a          899 c          814 g          500 t
ORIGIN      
        1 cgaagaggat tgacggtcca gccgtagact ccgacgtaca cgaggccgtc accaccggtc
       61 ttggtttccg cgaactcggc cgagatggtt cctagttgac tatgagtctt cgagcgatcg
      121 aactgcagtc cgacgcgcgc caaaaggtcc tcgactttgt tccaattggc cccgaaggcc
      181 gcgcctttgc cgtacaccgt catgcatccg gatccactgc cctccgccca aagctcgtag
      241 gagtaattgc cgctggcgtt acctttgctg ttcgagcagt agcgtcgccc acccgtcaag
      301 gccgcagcag gcgggcagct ggggctctca cccgctccgc ccgcccccgc gtcactcgta
      361 ttctccgcgc cacccgtgga ggaccggcct cccgcgttgg tgcccccaac gttgccgagg
      421 ccgccactac tgccgggcgc tccgccgttt cccagcgctc cgccgtttcc cagcgctccg
      481 ccgtttccca gcgctccgcc gtttcccggt gatccgctgt tgactccggc tccgccggca
      541 actggcaatc cggtgtcccc gcctgaagag tttgcgccat tcggaagagt tgaagtgccg
      601 ccgctagctc cgcttccaga cgaagcacca ctcatggtgt ggcctgaact agcgtgatcc
      661 gttgtcgttt cgacaacacc tcccgaagcc acgccacccg ctggccctcc actcgcactt
      721 ccactggcga ccccagcgct gccgccttcg cttgcagtcg agccagagtc aacacagctc
      781 gtaaaagcca cgtgcatgtg aaacgcaacg acactcatga tcccgaaaga tcgagcagct
      841 tggcgcatgc tgcgatatta aatgttccat tcgatagaat cgagaggaga gagcgctccc
      901 gagcgcaccg catcggcagc ttccggatgg cgcgcaggcc gtcacgcgaa aagtagacta
      961 tgcctggacg ctctctcgcg cggcgcttct gatcgatgcg cggcaagccg aggctctgga
     1021 gttacgcccc gggctccgcg ctcgagcatc gagcggttcg aaacggtccc cttcaacacg
     1081 gcgagctgct cgagggcagc atgccataca gggttgtcat cgcgtcgggc tgattcagca
     1141 gggccgcttc cccgaaccgc ggaggtttgc gtggctcgta acacttgcaa tggggagcaa
     1201 tggcaatgcg gacggggcgg gactaaattt ggattttgcg cctcctagtc cgaagccagc
     1261 aattcctcga tctagcctga cattgggcgc gcccgtgctc gctttggccc gccacggctg
     1321 cgcgcaccaa cagccaaact gaagccgatc ccgcgtcggg ccccgccgac ttcctccgtc
     1381 gtcggcgcga aatccagttc cccggtggaa ccaaacaaac tagccggagt aaatgattac
     1441 cgggaacgct ggaggcccgt aatgccgctt ggttccaaaa atgatggggg tagcgattcg
     1501 aggccagggt aagatgtgac tcggcccggc gaaagcgccg aggtgcccaa ccatgactgt
     1561 aaggtgcgtc aatggacttt cgacgaacaa gagcctagcg cttgacgcga gaggtcgggg
     1621 agagccgacg tgaagccaac cgcttttctg tcctctcggg tcgagcgact ggataaaatt
     1681 cacgatggca agcgctccgt cgtgtatcgc gcacgccttc gtgagtcgaa gcggccggtc
     1741 atcgccaagg tgctacgcgg gccgtatccg agccccgagc agcagtcgcg cttccgtcgg
     1801 gaatacgaag tcggtcgcgc cctgcacggc ccccatatca tcgaaaccat cgcgctcgaa
     1861 acgggcgagg atacgcttgc gatcctgatg gaggactgcg gcgccgagtc actcgcgggc
     1921 cgcgggccgc tcgaattagg ggagtttctg ccgctagctc gcgatatcgt gctcggcctc
     1981 gcgcatattc atgcctgcgc ggtcgcccac cgcgatatca atcccagcaa catcgtctgg
     2041 aatcgagggc ggaaactggt caagatcatc gactttggca tctctcgagc agtttcaaga
     2101 gagagcctcg agctggatcc caatgtcgat ccccacctcg aacgaggctt cgagggtacc
     2161 ctcgcgtata tctcacccga acagaccgga cgcatcaatc gttcggtcga tcaccggacg
     2221 gatctttact cgctcggcgt aaccttctac gagcttctaa ccgggcagcg gccgttcgcg
     2281 ggcgaggacg ccatggagct cattcatgcc cagatcgcgc gtgtgccggt ggcgccgcat
     2341 gaacggagag catcggtccc acgaccgctc tcggatctcg tgatgaagct tctggagaaa
     2401 aatcccgccg atcgatacca gagcgcgtcg ggtgtaaacg cagatctcga agaatgcagt
     2461 gcgcgacacg ctaggaccgg acacatcgca gggttcgagc tcgcgcggcg cgacaccgca
     2521 gctcgcctgc agctccccca gggcttgtac ggtagagacc acgagttcgc gagtttgcgc
     2581 aacgcgctcg cgcgcagcgc cgccggcaga tcccagatcg cgctcgtgag cggctattcc
     2641 ggaatcggca agacgcgatt ggtgcgggag ctccgcgggc ccacggcggc gctcggagga
     2701 tacttcgtct ccggcaagtt cgaacagttc agg
//
LOCUS       DASTKU010000023        10076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_895716, whole genome shotgun sequence.
ACCESSION   DASTKU010000023 DASTKU010000000
VERSION     DASTKU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10076
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895716"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="VFQ61_00650"
     CDS             complement(<1..1505)
                     /locus_tag="VFQ61_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HET9952974.1"
                     /translation="MSLSAENYEKMGVFYLGRGLDRAGSATTEPVLYPSKDLTTHGLC
                     VGMTGSGKTGLCLGLLEEAAIDQIPVIAIDPKGDLGNLLLTFPNLSASEFAPWVDAAQ
                     AEREGISIEALAEREAARTRAGLAEFGQEPARIARLMQSVERVIYTPGSRAGRPLALL
                     RSFSAPTQPAEREPELLRDRVQGIVSSLLSLAGIDADPLRSREHILLSQIFMQAFEAS
                     RDLSLPDLIRNVQKPSFTSVGVFELETFYPAKERLELAMSLNNLIAAAGAQALAQGEP
                     LEIAELLRSKTGKPKLSILSIAHLNDGERMAFVTTVLSELIAWMRTQPGTSSLRALLY
                     MDEIFGYFPPTAAPPSKQPMLTLLKQARAFGVGVMLATQNPVDLDYKGLSNCGTWFIG
                     RLQTERDKLRVLDGLEGAAASAGRAFDRSEMDRLLSGLRKRVFLMNNVHETAPRLVET
                     RFTLSYLRGPVTRDEIKRLMPASGGAAPSPAAPASDDAQNALPRTQPMPQVA"
     gene            1653..3386
                     /locus_tag="VFQ61_00655"
     CDS             1653..3386
                     /locus_tag="VFQ61_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9952975.1"
                     /translation="MLTASDRFRSRLLTALGLLPLACGGATTPLEPGGTGGNTSGGAT
                     GYSGATSVTGGASATSGGSVSSGGNPSTGGSTSRGGSTNKGGSTGKGGAPATGGSTPT
                     AGVGAVGGSTGGTAGGSTSVGGDGGVGGLPAPGPFHCENPTPYDPQGTILSCDGGFRH
                     RVSNDVCKSKLPRKNELPPSLYPEQDQCNSDADCVASEYGYCAGPGSELGPPINRCLY
                     GCVADSDCGSGQICECGDPIGVCVSSTCKTDRDCGADLLCATYIANPGCGGTAYACQS
                     LDDECASDQDCGANQQCSVRNGHRVCMLPTCVIGRPFLIEGRERLASVELRADFVDSG
                     ATNADLSGLSDDERRQLCEAWTRIGLMEHASIAAFARFALQLTSFGAPADLLTRAARA
                     MADETRHAQQAFSLASRYGGASLGPGRLDIQGSLADLDLENTVLTAFVEGCIGETVAA
                     LEAEVALSGAIDPAVRCVLHAVTEEETQHAELAWLFVQWALPQLGTSFAGKLRQSLLD
                     AQRAALAALLAEPETEGDATLRAHGILPEYQRRALRVRLLNEVISPCAESLLGKAPLA
                     PGRDPMIGFAA"
     gene            complement(3435..6590)
                     /locus_tag="VFQ61_00660"
     CDS             complement(3435..6590)
                     /locus_tag="VFQ61_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9952976.1"
                     /translation="MNISAIAIKRPVFTVMVTVALVVLGVVGFRSLGTDLFPDVTFPA
                     IVVTVPYPGASPAEVENLVTKPMEDGVIGLNGIDRVRSFSREGMSQVFVIFTLGVDID
                     EAATEVRERISQVKYKFPDDVKEPSIMRFDVSAAPVLTYTLSGQGSLSELRKYADDEL
                     RPSLEQVDGVAAVDVKGGATREVRVALDRAKVDALGLSPSAIVQRLKLENLNVPAGHF
                     DEGDREVSVRTIGEFNRVEQVRDTIVASAADGSSVRLRDVAQVDDGFADMRTRVIVNG
                     NEAVTLEVRKQSGRNTVEIAQAAKRKMALLEKSFPKNVRASLIIDQAHFIEENVQHVK
                     RDIAFGGAMAVLVILVFMLDIRSTLISALALPTSVIGTFFVMYVLGYTLNMMSMLGLS
                     LAIGLLIDDAVVVRENIFKHLERGKPPRQAALDGTKEVALSVLATTLTILAVFMPVSF
                     VKGIVGQFFRQFGITISAAVVISLFVAFTLDPMLSSRFSKSLTAKRDYFGWLKAPFEW
                     TFRQMENTYAAALSFALHRKLVIFVLAVGSLFFMGFITKLLGSEFVNAEDRGQFVVEA
                     ELPAGTSLDETMRWAHVADARMLENKQFRTVFTTVGPDEQINKIKWRVVASPKTERHE
                     TLADLKEVGRKAAAALPGAKIAVTDPPFVEGASTEAPIMINVRGTEFGEIERVANDIE
                     AILKSTPGVGDIQVRYSPGRPELSVHLDRQRAADRGLSLAQAAMALRTAMEGEEAGNM
                     RQGKDEIPIRVRLGESYRQDAQALANLTLTTPKGPIKLRDVANFERTEGPQVIERENR
                     NRQITVWAAPIGRPLGDVAKEFQPRIAALKMGPGMSIFYDGQLRLMNENNENMATALL
                     LGVVFIYIVLASQFESFIHPLTIMLTLPLAMVGAVLGLFLTGNTMAMGALIGIVLLMG
                     LVTKNAILLVDRAIVRVRDFGEPPLQAILEAGPERLRPILMTSAAMVLGMLPTAISNS
                     EGSEFRAPMAIAVIGGVISSTLLSLIVVPVFYLAIENLKARLRPKGARPGASRNESGA
                     PSVGTPAEPLVSSAAFSRTEY"
     gene            complement(6587..7813)
                     /locus_tag="VFQ61_00665"
     CDS             complement(6587..7813)
                     /locus_tag="VFQ61_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9952977.1"
                     /translation="MKSSESQVGLQNSVHASSSVPPGGVAARPRMPLVGMILGLAFVG
                     GLAAWTVQRVKAAQSVQQAVEQKRSAEQQRAKVQASAPEVVRVILGKPETWQPTVQFE
                     GTLQASQQAELGFKTGGRLGSIKGKLGDVVKAGAVLATLDTNEAAAQLKAANAQLRAA
                     EAQLALAADSEQRTQKLLDTGSVAAATGVQTTQQRALAEAQLDAARAQIGVVQVSLYN
                     HVLVAPFAGTLTRAPDGVGAVVTPGQVLFALADLRSLKLRGTISEPDANLLKLGSSVE
                     VDGGGPGARGKITALIGIVDASTRRVPIEAVLENASGSSLRAGSFVRAQALGGAPIQV
                     LRLPHEALRHGSQDEVFVVSNGALQARKLVFSVSKDGALLVRSGLQPNEPVVLSPRAE
                     ARSGAKVNIEAGSERP"
     gene            complement(7810..8478)
                     /locus_tag="VFQ61_00670"
     CDS             complement(7810..8478)
                     /locus_tag="VFQ61_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9952978.1"
                     /translation="MARPSDPQARSRLLNAAQRVFAERGLDRARVEEIAGEAGVSKGA
                     FYLHFASKEEAFREVVTTVISRLESFLSEDCLNQVAAHCSNPMPTEAKAALTAEPLDD
                     WLEGDVAIFEYVWEQRQLVRLVMEGGGSADYRHLVDHFADRVQSHVLSVLRLGVSAGL
                     YRSDLDVHVAAAFLAGGYDRFARRLVREPQKPDLRAVVRELQALVGRGIGSEQLIRKL
                     GNGT"
     gene            complement(8601..>10076)
                     /locus_tag="VFQ61_00675"
     CDS             complement(8601..>10076)
                     /locus_tag="VFQ61_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030248 - cellulose binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HET9952979.1"
                     /translation="QNETDIDCGGVCVPCSDQAACRKNLDCSSRLCLDDVCQPPSCSD
                     ETWNGYESDRDCGGTCDACIAGQHCSAPEDCQSSRCVMGICQPASCSDSILNGGETDV
                     DCGGSCEKGCDTGQICVNGADCLSKVCGAERCVAASCSDKVSNGDESGVDCGGSGSCS
                     RCPNGQACTQNSDCAQKYCDSNGVCASPGCSDGAVNGDETDKDCGGSCSSKCGTGAGC
                     KSAADCQSLSCVGSKCAAPSCTDKIKNQGESDIDCGGANCPDCDPGKICGSNADCTSL
                     VCGGGVCQAPTCSDGAKNGEETDLNCGGSKCAPCPDGMKCSLPSDCDSTMCSTTCQPN
                     LFEALYRQIDDSATDNEIQPAIGITNLSGATVDGAELMARYFYDDEGPTQENAWAAFE
                     VVNPAPGVSRTDFSITFDSSKQVKTYFDVNFKDFTISSGASSGLFQMQIHKRNWTPNY
                     IEANDWSYDATRTTTGVNRKIAIYRKQGTSWVRVFGLEPAL"
BASE COUNT         1809 a         3304 c         3125 g         1838 t
ORIGIN      
        1 gccacctgcg gcatgggctg ggtgcgcggc agggcgtttt gagcgtcatc gctcgccggc
       61 gccgcgggtg acggcgccgc gccgcccgat gcgggcatga gacgcttgat ctcgtcgcgg
      121 gtcacgggac cacgcaagta gctgagcgtg aatcgagtct cgacgaggcg cggcgccgtt
      181 tcgtgcacgt tgttcatcaa gaacacccgt ttacgcaggc ccgaaagcaa acggtccatc
      241 tcggaacgat cgaaggctcg accggcgctc gcggcggccc cctcgagtcc atccagcacc
      301 cgtagtttgt ctcgctcggt ctgcaagcgg ccgatgaacc acgtgccgca gttggagagc
      361 cccttgtagt cgagatcgac gggattctgg gtggcgagca tcacccctac cccgaaggca
      421 cgcgcttgct tgagcaacgt gagcatcggt tgtttcgagg gcggggccgc ggtgggtgga
      481 aagtatccga agatctcgtc catgtagagc agcgcacgca agctcgatgt gcccggctgc
      541 gtgcgcatcc aggcgatcag ctcgctcagc accgtcgtaa cgaacgccat gcgctcaccg
      601 tcgttgaggt gtgcgatcga gaggatactg agcttgggct tgccggtctt actgcgcagt
      661 agctcggcga tttcgagcgg ctcgccttga gcgagcgctt gggcgccggc agccgcaatc
      721 aagttgttga gcgacatggc gagttcgagg cgttccttcg cagggtagaa ggtctccagc
      781 tcgaagacgc ccacactcgt aaacgaaggc ttctgcacgt tccgaatcaa atcgggcagg
      841 ctcagatccc gactcgcctc gaaggcctgc atgaagatct gcgagagcaa gatgtgctcg
      901 cgactacgca gcggatcggc gtcgatgccc gcgagcgaca gcaggctcga cacgatgccc
      961 tgtacgcgat cgcgcaggag ctcgggctca cgctccgcgg gctgggtcgg cgcggagaag
     1021 gaacgcaaca aggcgagcgg cctgccggca cggctaccgg gggtataaat cactcgctct
     1081 acgctctgca tgaggcgagc aatgcgggca ggctcttggc cgaactcggc gagtccggct
     1141 cgagtccgtg ccgcctcgcg ttcggccaac gcctcgatcg agatcccctc acgctcggct
     1201 tgcgcggcat cgacccaggg cgcgaactcg ctcgcgctta gattcgggaa ggtgagcagc
     1261 agattgccga gatctccttt ggggtcgatg gcaatcacgg gaatctgatc gatggccgct
     1321 tcttcgagta gcccgagaca gaggccggtt ttgccgctgc cggtcatgcc cacgcacagg
     1381 ccatgggtgg tcagatcctt ggacggatag agcacaggct cggtcgtcgc gctgccagcg
     1441 cgatccaggc cgcgccccag gtaaaagacg cccatcttct cgtaattttc tgcgctgaga
     1501 ctcatgccta tcctcgccgt gatcgccggc gagttacgct gattgagccg tgcgtgcaag
     1561 cgtaagctgt gccagggcgt ggcacactca ggtgctttgc tcgggaaaga agcgaggcac
     1621 caaagggcac gaagcttgcc tagtggcgcc ccatgctcac tgcgtccgat cgattccgat
     1681 ctcggttgct cacggctctt ggtttgctgc ccctggcctg cgggggagcc acgactccac
     1741 tagaacccgg cgggactgga gggaacacgt ccggtggagc gacgggatac agtggcgcga
     1801 ccagcgtcac cgggggcgcg agcgcaacga gcggtggaag cgtatcgagc gggggcaacc
     1861 cgtccacggg cggttcgacg agcaggggcg gttcgacgaa caaaggcggt tcgacgggca
     1921 aaggcggcgc gcccgcaacc ggcggcagca cgccgaccgc tggtgtgggc gccgtcggag
     1981 gatctacggg aggaacagca ggcggctcaa cgagcgtagg tggcgacggc ggtgtcggtg
     2041 gcctccccgc gcccggccct ttccattgcg agaatccgac tccctatgat ccgcagggaa
     2101 caatccttag ctgcgacggc ggcttcagac atcgtgtttc gaacgatgtc tgcaaatcga
     2161 agctgccgcg gaagaacgaa cttccgccct cgctctaccc cgagcaggac caatgcaaca
     2221 gcgacgccga ttgcgtcgcg agcgagtacg gctactgcgc cggacccggt tccgaacttg
     2281 gaccacctat caatcgttgc ctgtatggtt gcgtcgctga ctcggattgt ggctcgggcc
     2341 agatctgtga gtgtggcgat cccatcggcg tgtgcgtgag ctcgacctgc aaaaccgacc
     2401 gcgattgcgg cgctgacctg ctgtgtgcga cctacatcgc gaacccgggt tgcggcggca
     2461 ccgcctacgc ctgccaaagc ttggatgacg agtgcgcctc tgaccaggac tgcggggcca
     2521 accagcaatg ctccgtgcgc aacgggcacc gcgtctgtat gttgcccacc tgcgtgatcg
     2581 ggcgcccctt cctaatcgaa ggccgcgaac gactagcgag cgtcgagctg cgcgccgact
     2641 tcgtggattc gggagcgacc aacgcggacc tctcgggttt gagcgatgac gagcgacgcc
     2701 agctgtgcga ggcctggacc cgcattggct tgatggagca cgcctcaatc gccgcgttcg
     2761 cccgcttcgc gctacagctc acgagctttg gcgcgccggc ggatctgttg actcgcgccg
     2821 cacgcgcgat ggcggatgag acccgtcacg cgcagcaagc gttttctctc gcgtcccgtt
     2881 acggcggcgc ctcgttggga cccgggcgtt tggacatcca aggtagtctc gctgatctag
     2941 atctcgagaa caccgtgctc accgccttcg tggagggatg catcggcgaa accgtcgcgg
     3001 ccctggaagc ggaagtagcc ctgagcggtg ctatcgaccc ggcggtgcgt tgtgtgctcc
     3061 acgccgtcac cgaggaagaa acccagcacg cggagctggc ctggctcttc gtgcaatggg
     3121 cgctcccgca gctcggcacg agcttcgctg gcaagttgcg tcagagcttg ctcgatgccc
     3181 agcgagcagc actcgcagca ctgctcgccg agccagagac cgagggcgac gcgaccctgc
     3241 gcgctcacgg catcctaccg gagtaccaac ggcgcgcgct gcgagttcgc ctgctgaacg
     3301 aggtgatctc gccttgcgca gagagcttgc tcgggaaagc cccactcgcg ccaggccggg
     3361 atccgatgat cggattcgcg gcctgacgcg atcgagtact gggtgcgtga actcagtact
     3421 cggtgcgtga gaactcagta ctccgtgcgt gagaacgctg ccgaagaaac caggggctcc
     3481 gccggcgtgc cgacgcttgg agcccctgat tcgtttcggc tcgcgccggg gcgagcccct
     3541 ttcgggcgca gacgcgcctt gaggttctcg atggctagat agaacaccgg cacgacgatt
     3601 aaggacagca gcgtggagct gatcacgcct ccaatcaccg cgatcgccat tggcgcgcga
     3661 aactcgctgc cttcgctgtt gctgatcgcg gtaggcagca tgcccagcac catcgctgcg
     3721 ctggtcatca agatcggacg cagccgttcg ggtcccgcct ccaagatggc ctgcagcggt
     3781 ggctcaccga agtcgcgcac ccgcacgatg gcgcgatcga ccagtaagat ggcgttcttg
     3841 gtgacgagcc ccatcagaag cacgatgccg atgagggcgc ccatggccat ggtgttgccc
     3901 gtcaggaaca ggccgagcac ggcgcccacc atcgcgagcg gcaacgtgag catgatcgtg
     3961 agcgggtgga tgaagctctc gaactgcgac gcaagcacga tgtagatgaa gaccacgccg
     4021 aggagcaggg ccgtggccat gttctcgttg ttctcgttca tcaagcgcag ctggccgtcg
     4081 tagaaaatcg acattccggg tcccattttc agggccgcaa tacgcggctg gaactccttg
     4141 gccacgtccc ccaacggacg cccaatcgga gcggcccaaa ccgtaatctg ccggttgcgg
     4201 ttctcgcgct cgatgacctg cggcccctcg gtgcgctcga agttggccac gtcacgcagc
     4261 ttgatcgggc ctttgggcgt ggtcagcgtc aggttcgcca gcgcctgcgc atcctgtcga
     4321 tagctctctc ccaaccgtac gcggatcgga atctcgtcct tgccttgccg catattgccg
     4381 gcttcttcac cctccattgc ggtgcgcagg gccatcgccg cttgagccag gctcaatccc
     4441 cgatcggcag cgcgttgccg gtctaggtga accgagagct ccggccgtcc cggggagtag
     4501 cgcacttgga tgtcccccac tcccggcgtg ctcttgagga tcgcctcgat gtcattggcg
     4561 acgcgctcga tttcgccgaa ctcggtgcca cgcacgttga tcatgatcgg tgcctcggtc
     4621 gaggcgcctt caacgaacgg cggatccgtc accgcgatct ttgctcccgg caaggccgcc
     4681 gccgctttgc gcccaacctc tttcaggtcc gcgagcgtct cgtgccgctc ggtcttcgga
     4741 gaggccacca cgcgccattt gatcttgttg atctgctcgt ctgggccaac cgtggtgaac
     4801 accgtgcgaa actgcttgtt ctcgagcatg cgggcgtcgg ccacgtgtgc ccagcgcatg
     4861 gtctcatcga gggaggtgcc ggcgggtaac tccgcttcca ccacgaactg cccgcggtcc
     4921 tcggcgttga cgaactccga ccccagcagc ttggtgatga aacccatgaa gaacaggctg
     4981 cccaccgcca agacgaaaat gaccagcttg cggtgcagcg cgaacgagag agccgccgcg
     5041 taagtgttct ccatctggcg gaaggtccac tcgaagggtg ccttcagcca accgaagtag
     5101 tcacgcttgg cggtcagtga cttcgagaag cgagacgaga gcatcggatc gagcgtgaac
     5161 gcgacgaaca gcgagatcac gacggccgcc gagatcgtga tcccgaattg ccggaagaac
     5221 tgaccgacga tgcccttcac gaacgacacc ggcatgaaca ccgcaaggat ggtcaaggtc
     5281 gtcgcgagca cgctgagcgc cacctccttg gtcccatcca gcgcggcctg ccgcgggggc
     5341 ttgccccgct cgaggtgctt gaagatgttc tctcgcacca ccaccgcgtc gtcgatcaaa
     5401 aggccgatcg cgagcgacag cccgagcatg ctcatcatgt tcagcgtgta gccgagcacg
     5461 tacatcacga agaacgtacc aatcacgctg gtcgggagag ccagcgcgga gatcaacgta
     5521 gatcggatgt ccagcatgaa caccaagatc accagcacgg ccatggctcc gccgaacgcg
     5581 atgtcgcgtt tgacgtgctg cacgttttct tcgatgaaat gcgcttgatc gatgatcaga
     5641 gaggcgcgca cgttcttggg gaagctcttc tcgagcaggg ccattttgcg cttggcggcc
     5701 tgggcaattt cgaccgtgtt gcgaccggat tgtttgcgca cctctagtgt cacggcttcg
     5761 ttgccgttga cgatcacccg ggtgcgcatg tccgcaaagc catcgtcgac ctgggccacg
     5821 tcgcgcagcc gcaccgaaga gccgtccgcg gcgctagcga cgatcgtgtc gcgcacctgt
     5881 tcgacacggt tgaactcacc aatcgtgcgg acgctcacct cgcgatcacc ctcgtcgaag
     5941 tgaccggcag ggacgttcag gttctccagc ttgagacgct gcacgatggc gctcgggctg
     6001 agccctagtg cgtccacctt ggcgcgatcc agcgctacgc gtacctcgcg cgtcgcgccg
     6061 ccttttacgt ctacggccgc aacgccatct acttgctcga gtgacgggcg cagctcgtcg
     6121 tcggcatact tacgaagttc cgagagcgag ccttggccac tcaaagtgta ggtgagcacc
     6181 ggcgcggccg agacgtcgaa gcgcatgatg ctcggctcct tgacgtcatc ggggaatttg
     6241 tacttcacct gtgagatgcg ctcgcgcacc tccgtcgccg cttcgtcgat gtccaccccc
     6301 agcgtgaaga tcacgaagac ctgcgacatg ccctcgcggg agaacgagcg tacgcggtcg
     6361 atgccgttca gcccgatcac cccgtcctcc atgggtttgg tgaccaggtt ttcgacttcg
     6421 gccgggctcg cgcccggata agggacggtc acgacgatgg ccgggaacgt cacgtcggga
     6481 aacaggtcgg tcccgagcga tcgaaagcca accaccccga gcacgacgag agccaccgtc
     6541 accatgacgg tgaagaccgg gcgtttgatc gcaatcgccg agatgttcac ggccgctcac
     6601 tccccgcttc gatgttgacc ttcgcccccg accgcgcctc ggcccggggc gagagcacga
     6661 cgggttcatt gggttgcaag cccgatcgca ccagcaaggc gccatctttc gagacagaga
     6721 acaccagttt ccgggcttgg agtgcgccat tgctcacgac gaatacctcg tcctgggagc
     6781 cgtgccgcag ggcttcgtgc ggcaagcgga ggacttgaat cggagcgccc cccaaggcct
     6841 gagctctgac gaagctgccc gcgcgcagcg agctccctga cgcattctcg agcacggcct
     6901 cgatgggcac gcggcgcgtg gaggcgtcga cgatgccaat caaggccgtg atcttgccgc
     6961 gggcgccggg cccaccgcca tccacctcga cgctgctccc gagtttgagc aggttggcgt
     7021 cgggttccga gatcgtgccg cgcagcttca gcgagcggag atccgcaagc gcgaacagca
     7081 cttgaccggg agtgaccacc gcgcccaccc cgtctggcgc gcgcgtcagg gtgccggcga
     7141 acggcgcgac cagcacgtgg ttgtacaaac tgacctgcac gactccaatc tgcgcgcgcg
     7201 cggcatcgag ctgcgcttcc gcgagcgcgc gttgctgcgt cgtctgcacg ccggtcgcgg
     7261 cggcgaccga gcccgtgtcc aacagcttct gagtgcgctg ctccgagtct gcggccagcg
     7321 ccagttgagc ctcggccgca cgcagctgcg cgttggcggc cttgagctgt gcggctgcct
     7381 cattcgtgtc gagggttgcc aacacagcgc ccgctttgac cacgtcgccg agctttccct
     7441 tgatgctccc caggcgtccg ccggtcttga aacccaactc cgcctgctgc gaggcctgaa
     7501 gcgtgccttc gaattgcacg gtgggttgcc aagtctccgg tttacccagg atcacccgca
     7561 cgacctccgg agccgaggct tggaccttgg cccgttgctg ctccgcggag cgcttctgct
     7621 ccactgcctg ctgcacgctc tgcgcagctt tgacgcgctg aaccgtccaa gccgccaggc
     7681 cgcccacgaa agccaagccc aagatcatcc ccaccaacgg catccgcggt cgagccgcga
     7741 cgccgcccgg gggtacgctc gaagaagcgt gcaccgagtt ctgcaaccca acctgcgatt
     7801 cactggattt catgttccgt tcccgagttt tcgaatcagc tgctcgctgc caatcccgcg
     7861 acccacgagc gcttgaagtt ctctgaccac cgcgcgcaag tcgggcttct gtggctctcg
     7921 caccaagcgc cgtgcaaagc gatcgtagcc acccgccaag aatgcagccg cgacgtgcac
     7981 gtccaagtca ctgcgataca gtcctgccga cacgcccagc ctcaaaacgc taagcacgtg
     8041 gctctgcaca cgatccgcga agtgatcgac caagtgacga tagtccgcgg aacctccgcc
     8101 ctccatgacc aaacgaacga gctggcgctg ttcccaaacg tattcgaaaa tcgccacgtc
     8161 gccctccaac cagtcgtcca gaggctcggc tgtgagcgct gccttcgctt cggtcggcat
     8221 cggattggag cagtgggcgg cgacctggtt caagcaatct tccgacaaga aggattcgag
     8281 ccgcgagatg accgtcgtga ccacctcgcg aaacgcctct tctttgctcg cgaagtgcag
     8341 gtagaaggcg cccttcgaga cccccgcctc ccccgcaatt tcctccactc gagcccggtc
     8401 gagccctcgt tcggcaaaca cgcgctgagc tgcattgagc agccggctac gggcctgagg
     8461 atcgctagga cgagccatcg ggagggacag aatggcctgc gaaaaatcag gccagagtaa
     8521 atgaccgcca ggtcagtgac cggggagtca tttattcggc gcggtccccc ttcgtcaaga
     8581 cgttcgctgg gcgattcgtg tcagagcgca ggctctaggc cgaacacacg tacccagctc
     8641 gtaccttgtt tgcgatagat cgcgatcttc cgattcaccc cggtcgttgt acgcgttgcg
     8701 tcgtaggacc agtcgttcgc ctcgatgtag ttcggagtcc aattgcgttt gtgaatctgc
     8761 atttggaaca ggcccgaact ggcaccactg gagatcgtga agtccttgaa gttgacgtcg
     8821 aagtaggtct tcacctgctt tgacgaatcg aacgtgatcg aaaagtccgt tctgctcaca
     8881 ccgggcgctg ggttcaccac ctcgaacgct gcccaggcgt tctcctgagt gggcccttcg
     8941 tcatcgtaga aatagcgcgc catgagctca gccccgtcca cggtggcacc cgacaagttg
     9001 gtgatgccga tcgcaggttg gatctcgttg tccgtcgccg agtcgtcgat ctggcggtaa
     9061 agcgcctcga acaggtttgg ttgacaggtg gtgctgcaca tcgtgctgtc gcagtccgag
     9121 ggcaagctgc acttcattcc gtcaggacac ggcgcgcact tactgccgcc acagttcagg
     9181 tccgtctctt ctccgttctt cgcgccatca ctgcacgtcg gcgcctggca aaccccacca
     9241 ccgcagacca gactcgtgca gtcggcgttg gaaccgcaga ttttgccagg gtcgcagtcg
     9301 gggcagttgg ctccgccgca gtcgatgtcg gactcgccct gattcttgat cttgtcggtg
     9361 cagctggggg ccgcgcactt gctcccgacg cagctcaagc tttggcagtc ggccgccgat
     9421 ttgcagcccg ctcccgtccc gcacttgctc gagcaggacc cgccgcagtc cttgtcggtc
     9481 tcatctccgt tcaccgcgcc atcgctgcag cccggtgacg cgcacactcc gttcgagtcg
     9541 cagtacttct gagcgcagtc gctgttctgg gtgcaagctt ggccgttcgg gcaacgcgag
     9601 cagctgccgg aaccgccgca gtcgacgccc gactcgtccc cattcgaaac cttgtcgctg
     9661 cagctcgccg cgacgcagcg ctcggcgccg caaaccttgc tcaagcaatc ggcaccgttg
     9721 acgcagattt ggccggtgtc gcagcccttc tcgcagctgc caccacaatc cacgtcggtc
     9781 tcgccgccat tcaggatcga atcgctgcaa gatgcgggtt ggcaaatccc catcacgcaa
     9841 cgcgagctct gacaatcctc gggcgcactg cagtgttgcc cagcaatgca tgcgtcacac
     9901 gtcccgccgc aatcccggtc ggactcgtac ccgttccaag tctcatcgct gcagctgggc
     9961 ggctggcaaa cgtcgtccaa gcaaagccgg ctcgaacaat ccaggttctt gcgacacgcg
    10021 gcctgatcgg agcagggcac gcagacgccg ccgcagtcta tgtccgtttc attctg
//
LOCUS       DASTKU010000024        12468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_226730, whole genome shotgun sequence.
ACCESSION   DASTKU010000024 DASTKU010000000
VERSION     DASTKU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12468
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226730"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="VFQ61_00680"
     CDS             complement(<1..1285)
                     /locus_tag="VFQ61_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9952980.1"
                     /translation="MAHIDAGKTTCTERILFYTGINYKIGEVHEGAATMDWMEQEQER
                     GITITSAATNCFWTPNGGPNEGKKHRINIIDTPGHVDFTIEVERSLRVLDGAVAVFDG
                     GNGVEPQSETVWRQADRYSVPRIAFVNKMDKVGADFQMNLDSMRERLGCVPVPVQWPV
                     GQEEEHRGMVDLIRMKAAIFDDDTMGSKYSWEDVPADLVEKCRDLRERLIEACADIDD
                     QIMEKFLDGRAAEVTEQELYAALRKGTCSFKFVPVMCGSAFRNKGVQLLLDGVVNFLP
                     SPVDIPPVVGTSPDDESKKIERKADDDAPFAALAFKIMNDPFVGNLTFFRIYSGTLTS
                     GTAVLNSTRGKRERIGRILRMHANKREELKECNAGNIYAAVGLRDTRTGDTLCDDKHP
                     VVLERMVFPEPVISIAIEPRTQADLDKLGISLGKLM"
     gene            complement(1631..2101)
                     /gene="rpsG"
                     /locus_tag="VFQ61_00685"
     CDS             complement(1631..2101)
                     /gene="rpsG"
                     /locus_tag="VFQ61_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET9952981.1"
                     /translation="MPRRRDVPKRKILPDPKYKDKLVAKFTNSIMLGGKKAVAEGILY
                     GAFDVIQARFKEDPLEVFRKALDNIKPKLEVKSRRVGGATYQVPVEVRPERRVALAMR
                     WLVTYSRDRGEKTMRERLAAELVDAAQMRGNAVKKKDDTHKMAEANRAFAHYRW"
     gene            complement(2125..2523)
                     /gene="rpsL"
                     /locus_tag="VFQ61_00690"
     CDS             complement(2125..2523)
                     /gene="rpsL"
                     /locus_tag="VFQ61_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET9952982.1"
                     /translation="MPTIQQLIRSGRESLITRTASPALRSCPQKRGVCLRVYTTTPKK
                     PNSALRKVCRVRLTNQMEVISYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHVVRGTL
                     DASGAIGPSSTNKANRNRKRSKYGVKRPKK"
     gene            complement(2845..4020)
                     /locus_tag="VFQ61_00695"
     CDS             complement(2845..4020)
                     /locus_tag="VFQ61_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952983.1"
                     /translation="MMKTSRLAWVLGSFTWLLSSCASDSASSAGGEFEGAPELAAFEL
                     NLSDDAEAEGLATEVDAVDPAADSVTSALAALSSALEQESAPELLRARHAIRDLNQAI
                     RAFMQPIAALLRGTDPNAPLPRIREWGPVKRGQAEYRFVLRQGSERRYGWRLEARPAG
                     STDAFSIVAAGHVRVGAVARRGVGSLGLDLDALRIVDPTVHAAGKLYARFAHGPVATR
                     LSYGLHEFTPNPEQNSGVDAWVQGVHVSTGFNRVRLAFHGNLPETATDAEELVLMRVR
                     HHRAEGGRADAIVSGGDVPDGKLWVVSECWAPELASVYRIVRECERGEAVSELVAAGS
                     ACTVVKTTGDRSACPGALRDPQLPPSDPNAADVDMDDGEETLLPPDAMPSGEADVED"
     gene            complement(4201..5019)
                     /locus_tag="VFQ61_00700"
     CDS             complement(4201..5019)
                     /locus_tag="VFQ61_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952984.1"
                     /translation="MKPVVCWLRRVSSLAALVLGAGCSSPTESTGVGNPSLTLALVTD
                     ERAEPTLVGAGGGSGTDVEVDAGEDEGLQSSAVHQAILVFGGLRLLPCDPALPPTVVP
                     GRFVVDLKRQTTEPELPAIPGVSGGYCEIDVPLVRATAPAEIAGTSIYFAGERSDGTE
                     FILYADVRAVLRLRATPGGAWDPERTPHLLWAFRPRRWLLRQELESAAATLDEDGARV
                     IVIDIDRHPLLFRAIKARIAGRSSLHLDRDNDGVLDEDERDSSALLGQGLEDTE"
     gene            complement(5016..5891)
                     /locus_tag="VFQ61_00705"
     CDS             complement(5016..5891)
                     /locus_tag="VFQ61_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HET9952985.1"
                     /translation="MTTRRAPKRNTESTVSVYEYLDFRAYLRAYYEAEKKRRPSFSFR
                     LFSKLAGLRSPNFLKLVMEGERNLGPESVPRFAEALGLSAQDAEFFADLVAFGQAQSM
                     ADKNRSFERIAGSRRFRKARRIEGDLFAYLSHWYNPAIRELAARDDFQEEPRWIAAQL
                     RPTITPAEAAEAMRLLLSLGLLTRDAEGRVARGEPTLTTEHEVRSLGAAAFHRQMLER
                     AAQSIETVPRDKRDLAALTVCVSPETAALVKQKIQKFRETLTELCDSDPQGSVVYQLN
                     VQWFPLSAEKDDLPR"
     gene            6173..6982
                     /locus_tag="VFQ61_00710"
     CDS             6173..6982
                     /locus_tag="VFQ61_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952986.1"
                     /translation="MLRLARAVRLFRPAATALAALPWLWVSSAAAKPTHQFEAMSASA
                     KTDAHTKGITGLREAWSAFADGWLLLDMVVVLLLALLLGAVIAYHPSARRRVTSLEQF
                     EQPKTLLLYSVVAAVVALIVEVQPAMAFVIFGIGGLLRFRTEVGEAKDTGRVILVTVL
                     GLCCGLKIFIVALPATVIGWLLIYALEMQPAGIIRVSGVGEHAMHAATQAYRAKISAA
                     GFTIIGEQTKFIKREFVFVVKAPPLLDRESLRAEFDRMPPEVRGVVDWERI"
     gene            complement(6996..7532)
                     /locus_tag="VFQ61_00715"
     CDS             complement(6996..7532)
                     /locus_tag="VFQ61_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952987.1"
                     /translation="MSQAARVAGAFAELERHTVQTRAVLQRVLLQAITDPHVIAATAE
                     APLADYLRVLGRSLETLPIRVLHVCPVLPDFGSVAHHFTAATRVAAEAHVQSLPRPLR
                     ERVLWVASSAQPELNRLFGESADLALFSRLAGLALADLPFLVHVLEGEPVEPFLTATS
                     SAPPQSASRMRASRKPGN"
     gene            complement(7532..7906)
                     /locus_tag="VFQ61_00720"
     CDS             complement(7532..7906)
                     /locus_tag="VFQ61_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952988.1"
                     /translation="MVPGESSSGHLLNREALRRLELAHDAGWTADELAELYNELKIQI
                     ENTCPGWRADRVKCSDGSEAFVGALGPVLLVLPDRGLFIGHLGATAADGALFYTGMVM
                     SPDGCLMFPPPNTSCPGTRRVR"
     gene            complement(8039..8479)
                     /locus_tag="VFQ61_00725"
     CDS             complement(8039..8479)
                     /locus_tag="VFQ61_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952989.1"
                     /translation="MPNDIVEELAGCLVLGELLSRVRERWGAFRLLDHFKQGEFHHDV
                     LIELPEGSGLQDRILVVSTNCNGGVKEVLLLREPPTAGGLWRARCPENSEFEGPLPEI
                     TARARTIHWFDPCELLTADARSEYRPEYRERQCGGGWVARPRAA"
     gene            complement(8587..8991)
                     /gene="mscL"
                     /locus_tag="VFQ61_00730"
     CDS             complement(8587..8991)
                     /gene="mscL"
                     /locus_tag="VFQ61_00730"
                     /inference="COORDINATES: protein motif:HMM:NF001843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET9952990.1"
                     /translation="MGVLQEFREFAIKGNVIDLAVGVVIGAAFGKIVSSLVADVITPP
                     LGLIVGGVDFSHLKVVLKAAADGKPPVTLNYGVFLQATFDFVIVAAAIFLLVKGINQL
                     KKKPPEAPKAPAPPSPEVLLLTEIRDVLKERR"
     gene            complement(9214..10734)
                     /locus_tag="VFQ61_00735"
     CDS             complement(9214..10734)
                     /locus_tag="VFQ61_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9952991.1"
                     /translation="MFQIRMCRSSVLACSAGVLLSASIARAQWSLDSFEPSAPGSTTL
                     SGSNAEFEADSPVLRTLASYAHGPLVFRRSTGDAPEVERALSLHLQGTYAPSRYLGFD
                     LGLPMLLQGGHGQAALGTPDPSGLALGDVRLGARLPVLFGAASGLAAAFSAQAWLPTG
                     SAAQVAGTGQVRYGLSALFSGEAGELAWSAGVGRRRLANADSERKASELFGTHTSFFA
                     GARVRSLRATQGWSFGPELFGAAPDHIAPSRRVSLELLLAGAVKRGPWSFTAAIGPGL
                     TRGPGTPRFRVVLEVSFDLSEAERAATSPVAGFESRRLEPATELEFRLRPANTRRVST
                     HDMLAHSGAESELEPTSNPSNSVERADDCEVPDQADAAPESNNCALSVRLEEERIAVA
                     NPIRFTSGRDRFDPSSEQTLSAIAELLGAHPEIERVSVEGHTDDIGSKALNLALSRRR
                     ADAVVRWLIEHGVDSGRLESQGLGPESPLDPSPTAGARERNRRVEFRVLKTRPIAR"
     gene            complement(10893..11642)
                     /locus_tag="VFQ61_00740"
     CDS             complement(10893..11642)
                     /locus_tag="VFQ61_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952992.1"
                     /translation="MLSVRPGLFVLLLGFAACSRPKPQPELEGHLPPLSGATWLMNLE
                     PSGARVSVPLGATEARPIVIALHGSHDRPEWTCGSYRHASGNRAFVLCPAGKPLADGR
                     FGLLDLADTHGRVREGLQSLKARFGKHVAAGRVVLAALGPAVDQALELALQEPTFFSY
                     LLLIDGSPKRFTAPMIARFGSGGGKRVLWLCSQPDCAAEVEPRLRAPAPAGVELRWLF
                     PAKSHGLDEEMVRVLAREFPWLVAGDRRFSR"
     gene            complement(11751..>12468)
                     /locus_tag="VFQ61_00745"
     CDS             complement(11751..>12468)
                     /locus_tag="VFQ61_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952993.1"
                     /translation="GSLGHIPLSRIRGYRLARVNLESGELERDARGFAIECKPGELGE
                     LLLRLRVRPDSSTGDYTGYLGESERPDRIARDVFRPGDLYCRSGDLMRRDRLGYYYFV
                     DRLGDTFRCKGHNVSTREVEDALSRTPGVRMLSCTGLRLPSHDGKFGLLTLAVANDFR
                     MADLERQLPRLPPYAQPGFLRFVPEVALTSSLKAKRVPVLIEDLSPGSGAQAFWRQGD
                     HFVPLTRADYERIAAGQQRF"
BASE COUNT         2368 a         4058 c         3804 g         2238 t
ORIGIN      
        1 acatcagctt gcccaagctg atcccgagct tgtcgagatc ggcttgggtc cggggctcga
       61 tcgcgatcga gatcaccggc tcggggaaca ccatgcgctc cagcacgacc gggtgcttgt
      121 cgtcgcacag cgtgtcaccc gtacgggtat cgcgcaggcc tacggccgcg tagatattgc
      181 cggcgttgca ctctttcagc tcttcgcgct tgttggcgtg catgcgcagg atgcgcccaa
      241 tccgctcgcg cttaccgcgc gtcgagttga gcaccgccgt accgctcgtg agcgtaccgg
      301 aataaatccg gaagaaggtc aggttcccga cgaacgggtc gttcatgatc ttgaacgcca
      361 gggccgcgaa gggcgcgtcg tcgtcggctt tgcgttcgat cttcttgctc tcgtcgtccg
      421 ggctcgtgcc gaccacgggc ggaatgtcca ccggcgatgg caggaagttc accaccccgt
      481 cgagcagcag ctgaaccccc ttgttgcgga aggccgagcc gcacatcacg ggcacgaact
      541 tgaaagagca ggtgcccttg cggagcgcgg cgtagagctc ctgttcggta acctcagcgg
      601 cgcgaccatc caggaatttc tccatgatct ggtcgtcaat gtccgcgcac gcctcgatca
      661 gtcgctcgcg caggtcacgg cacttctcca ccaagtccgc cggtacgtct tcccagctgt
      721 acttggagcc catcgtgtca tcgtcgaaga tcgccgcttt catgcggatc aaatcgacca
      781 tgccgcgatg ttcctcttct tgccctacgg gccactgcac gggcacgggc acgcagccca
      841 aacgctcgcg catcgagtcg aggttcatct ggaagtcggc gcccaccttg tccatcttgt
      901 tgacgaacgc gatgcgcgga acgctgtagc ggtccgcttg gcgccagacc gtttcgctct
      961 ggggctctac gccattaccg ccatcgaaca ccgcgaccgc gccgtcgagc acgcgcaagc
     1021 tgcgctccac ctcgatcgtg aaatcgacgt ggccgggcgt gtcgatgatg ttgatacgat
     1081 gcttcttgcc ctcgttcggg ccgccgttgg gggtccagaa gcaattggtg gccgcactgg
     1141 tgatggtgat gccacgctct tgctcctgct ccatccagtc catggtggct gcgccttcat
     1201 ggacctcacc aatcttataa ttgatgcccg tgtaaaacag gatgcgctcg gtacacgtgg
     1261 tcttgcccgc atcaatgtgg gccatgatgc cgatgttccg agtgcgttcg aggctgattt
     1321 cacgggccat ctggatattt ccttttcgga tcgcgtgacg gacgaaaccc cctccggtct
     1381 ccgacctccc gcgttttggg aggttttgat gttaccggaa gggggctcgg ggtgcgttgt
     1441 tgccgccgca gcgggagtac gaggaaacgt gctcggactc ttctcagcaa cccggtcctt
     1501 atgtcgtctg acgcatgctc tccgacgaat ctcctaaagg ttcagtgaat ctcgctcgcg
     1561 actggagcca ttcggcaccg accggtgaaa aaagcttagc cggcgaatct cttcaccggc
     1621 tgctcgtcac tcaccagcgg tagtgagcga aggcgcgatt ggcctccgcc atcttgtgcg
     1681 tgtcgtcttt cttcttcacg gcgttgccac gcatttgagc ggcatccacc aactcggcag
     1741 caaggcgctc gcgcatcgtc ttctcgccac ggtcgcgcga gtaggtcacg agccaacgca
     1801 tggccagagc cacgcgacgc tcgggtcgca cttcgaccgg cacctggtaa gtggcaccac
     1861 ccacgcggcg gctcttcact tccagcttcg gcttgatgtt gtccagcgcc ttgcggaaaa
     1921 cctccagcgg gtcctccttg aagcgagcct gaatcacgtc gaacgcgccg tacaggatgc
     1981 cttcggcgac cgcctttttg ccacccagca tgatgctgtt ggtgaacttg gcgaccagct
     2041 tgtccttgta cttggggtcc gggagaatct tgcgttttgg aacgtcgcgg cggcggggca
     2101 tgacagactc ctatcgctcc gaaatcactt cttgggccgc ttgacgccgt acttcgagcg
     2161 cttgcggttg cggttggctt tgttggtgct gctcggcccg attgctcccg acgcatcgag
     2221 agtgccgcgc accacgtgat agcgaacacc cggcaggtcc ttgacgcggc cgccacggat
     2281 cagcaccacg ctgtgctcct ggaggttgtg accttctcca gggatgtagc tgatgacttc
     2341 catctggttc gtcagacgga cgcggcacac cttacgcagt gcggagttcg gcttcttcgg
     2401 ggtcgtcgtg taaacgcgca agcacacgcc gcgtttctgc gggcacgagc gcagtgccgg
     2461 ggaggcggtt cgcgtaatga gcgactccct accggagcga atgagctgct gaatcgtcgg
     2521 catcgaaagc gattttctgc gagttcggga agggcctgtg ggtttttggg gaacggcagg
     2581 gtagccgcag actacgccat gtcaagtctc cttgagcgtt gcgtggtctc cgagcgcaat
     2641 ccccgcggtt ttgcgaggct cgttcgtgag gcccggtagg tacccagact gaacatcgga
     2701 gtagccgccg gacctttcga gcgcgcccct cttagcgcca gcgcgaatgg aatggaagtc
     2761 cattgggcac gcgtttcatc ttcgtctcga atctggcctg cccccgcggc cgctccccaa
     2821 ggtcgaggac ccttcgaatt ggcctcaatc ttcgacgtcg gcctcgccgc tcggcatggc
     2881 gtccggcggc agcaacgttt cttcaccgtc gtccatgtcg acgtccgcgg cgttcggatc
     2941 gctcggaggg agctgcgggt ctcgcaatgc acctggacat gcgctccggt ctccggtggt
     3001 tttgaccacg gtgcacgccg agccagccgc gacgagttcg gagacggcct cgccccgctc
     3061 gcactcgcgg acgattcgat agacggatgc gagctctgga gcccagcact cactgacgac
     3121 ccacaacttg ccgtctggaa cgtcgcctcc actgacgatc gcgtccgcgc gtccgccttc
     3181 ggcccgatgg tgccgcacac gcatgagcac gagttcctcg gcgtcagtgg ccgtctccgg
     3241 caagttgcca tggaacgcga gcctcacgcg gttgaagccc gtgctgacgt gtacgccttg
     3301 tacccaagcg tctactccgc tgttctgctc ggggttcggg gtgaattcgt ggagcccgta
     3361 ggacagtcgc gtggcgaccg ggccgtgagc gaagcgcgcg tacaactttc ccgccgcgtg
     3421 caccgtcggg tcgacgatcc gaagcgcgtc gagatccaac cccaagctgc ccaccccgcg
     3481 ccgcgcaacg gcgccgactc gaacgtgccc tgcagcgacg atcgaaaatg catccgtaga
     3541 cccggcgggt cgcgcttcca accgccagcc atagcgccgc tcgctgcctt gacggagtac
     3601 gaaacggtac tctgcctgcc cacgcttgac ggggccccac tcgcggatgc ggggcagcgg
     3661 agcgttgggg tccgtaccgc gcagcaacgc ggcaatcggt tgcatgaagg cgcgaatcgc
     3721 ctggttgaga tcgcgaatgg catggcgcgc tcggagcagc tcgggtgcgg attcctgctc
     3781 gagagcgctc gagagcgccg ccaacgccga ggtcacgctg tctgcggcgg gatcgaccgc
     3841 gtcgacttcg gtcgcgagcc cctcggcttc cgcatcatcg ctcaggttga gctcgaacgc
     3901 tgcgagttcc ggtgcgcctt cgaactcacc tcccgcggaa ctcgccgagt ccgaagcgca
     3961 gctcgaaagc agccaagtga acgaacccaa cacccacgct aaacgactag ttttcatcat
     4021 ggcatcaact ccttttcggg gaacgagaaa caccgccaca ggcacacgcg cgcacgcaca
     4081 cccagcctgc gattcgcgga agaaaaagat cgagggctgc gcgcgcaacc ctccgcagcg
     4141 gattactggc gaggatccga aactaacgaa ctcttcgttc ccccgcgggg ggggccgaat
     4201 ctattcggtg tcttcgagcc cttgcccgag taacgcactc gaatcgcgct cgtcttcatc
     4261 gagaacccca tcgttatcgc gatccaggtg cagtgagctt cgaccggcga tgcgcgcttt
     4321 gatcgcgcga aacagcagcg ggtgccgatc gatgtcgatg acgatcacgc gcgcgccatc
     4381 ttcgtccagc gtagccgcag ccgattccag ctcctgacgt aagagccagc ggcgcgggcg
     4441 aaacgcccac agcaagtgcg gagtacgctc gggatcccag gcaccgccgg gggttgcgcg
     4501 caagcgcaat acggctcgaa cgtcggcgta gagaatgaac tcggtaccgt cggagcgctc
     4561 acccgcaaag tagatcgagg tgcctgcaat ttcggctggt gccgtcgcgc gcaccaacgg
     4621 cacgtcgatc tcacaatagc cgccgctcac gcccggaatc gccggcaact cgggctcggt
     4681 ggtctgccgc ttcaaatcta cgacgaaccg tccgggaacg acggtgggcg gaagagcagg
     4741 atcgcagggc agcaagcgta gccccccgaa gacgaggatt gcctggtgca cggcactgct
     4801 ctgcaagccc tcgtcctctc cggcgtccac ttcgacgtcc gttcccgaac cgcccccagc
     4861 gcccacaagc gtgggctccg cgcgctcgtc cgtcactaga gcaagcgtga gactcggatt
     4921 gccgacgcca gtcgactcgg tcggagagga gcagccggcg cccagcacca acgcggccag
     4981 ggagctcaca cgacgcagcc agcagaccac gggtttcatc ggggaagatc gtccttctcg
     5041 gcagatagag gaaaccattg cacgttgagt tgataaacga cgctcccttg cggatcggag
     5101 tcacacagct ccgtgagcgt ctccctgaac ttctggatct tctgcttcac gagcgccgcc
     5161 gtctccggac tgacacatac cgtcaacgcg gcgagatctc gcttgtcgcg cggcacggtt
     5221 tcaatcgatt gcgccgcgcg ctcgagcatt tgacggtgaa aggcggctgc gccgagcgag
     5281 cgcacctcat gctcggtggt caacgtcggc tctccgcgcg ctacccgtcc ttccgcgtcc
     5341 cgggtcagca agcccaagga cagcagcaac cgcatcgctt ccgcggcttc tgcaggagta
     5401 atggtgggac gcagctgcgc cgcaatccag cgcggctctt cctggaaatc atcgcgcgcg
     5461 gccagctcgc gaatggcggg gttgtaccaa tgcgagagat aagcgaacaa gtccccctcg
     5521 atgcggcgcg ctttacggaa ccggcgcgaa ccggcgatgc gctcgaagga ccggttcttg
     5581 tcagccatgc tttgggcttg gccaaatgcg acgagatcgg cgaagaactc cgcgtcctga
     5641 gcgctaagcc ctagtgcttc cgcaaatcgc ggcactgact cggggccgag gttgcgctct
     5701 ccttccatta ccaacttgag aaagttgggc gagcgcagcc cggccaactt ggagaacaac
     5761 ctgaacgaaa agctcggccg acgcttcttt tcggcctcgt agtaggcccg caggtaggct
     5821 cggaagtcga ggtattcgta gacgctcacc gtcgactcgg tgtttctctt gggtgcgcgt
     5881 cgggtcgtca tctcgaaacc attctggcgc gcgcttagcg accttcaagg caacgcggcc
     5941 agattcagta tacacgccga atacaaggtg gcggttttta gcctgattat tgccgccatt
     6001 cattcgcgcc atcgaatcat tggaatacac actcgggtcc tgcggtgacc acggtcccct
     6061 acccgctgca aaccttttga acggctcgcc tcgacagaac gcaggtcaaa gacgaatccg
     6121 ttgccttccc aacacgattt gccgttacac cggcttggtg ttgagtacgc ccatgttgcg
     6181 cttagctcga gccgtccgtc tgtttcgccc tgcggctact gccctggccg ccctgccctg
     6241 gctgtgggtc agcagcgcgg ccgcgaaacc gacgcatcaa ttcgaggcca tgtctgcttc
     6301 agccaaaacc gacgcgcata ccaaaggaat caccggactg cgtgaagctt ggtctgcttt
     6361 cgccgacggt tggctgctgc tggatatggt cgtggtgctg ctcctcgcgc tgctgctcgg
     6421 cgcggtgatc gcgtatcacc cttcggctcg gcgccgagtg accagcctcg agcagttcga
     6481 gcaaccaaag acgttgttgc tctactcggt ggtcgcggcc gtggtcgcgc tgatcgtgga
     6541 agttcagccg gcgatggcgt tcgtaatctt cgggatcggc gggctgctgc gcttccgcac
     6601 cgaagtcggc gaggccaagg atacgggtcg cgtgattttg gtgacggtgc tcggactgtg
     6661 ctgtgggctg aagatcttca tcgttgcgct gccggcgacc gtcatcggct ggctgctgat
     6721 ttacgccttg gaaatgcagc ccgcgggcat cattcgcgtc tccggtgtcg gcgaacacgc
     6781 gatgcacgcc gccacgcagg cttatagagc caagatctcc gccgcgggct tcacgatcat
     6841 tggcgagcaa accaagttca tcaaacgcga gttcgtgttc gtcgtcaaag cgccgcccct
     6901 actcgatcgt gaatcgctgc gggccgagtt cgatcgcatg ccgccggaag tccggggcgt
     6961 ggtggactgg gagcgcatct aacgaagcgg ccgcgttagt tacccggctt gcgggatgct
     7021 cgcatccgag acgcgctctg aggcggggcg gagctcgtgg cggtgagaaa cggctcgacc
     7081 ggttcacctt cgagcacgtg caccaagaac ggcaaatcag ccaaggccaa gcccgccaat
     7141 cgcgaaaaca aggccaggtc agcggactcg ccgaacaggc gattcagttc gggctgcgcc
     7201 gagctcgcca cccacagcac gcgctcgcgc aatgggcgag gcaggctctg cacatgggcc
     7261 tccgccgcca cgcgggtggc cgccgtgaag tgatgggcga cggaaccgaa atccggcaac
     7321 acggggcaga cgtgcaaaac gcggattggt aaagtttcca ggctgcgccc gagtactcga
     7381 aggtaatcgg cgagcggagc ctctgcggtg gcggcaatca cgtgaggatc ggtgatggcc
     7441 tgcagcaaca cgcgctgaag cacggctcga gtctgcactg tgtgtcgctc taattcggcg
     7501 aacgcgcccg caactcgagc cgcttgggac atcagcgaac cctccgggta cctgggcaac
     7561 tcgtgttggg cggagggaac atcaagcatc catccggaga catgaccatc ccagtataga
     7621 acaacgcccc gtcggctgcc gtagctccga ggtggccaat gaaaagtcct cgatccggca
     7681 gcacgagcaa cacgggcccc aatgcgccca cgaatgcctc gctgccatct gaacacttga
     7741 cccggtctgc tctccagccg gggcaggtat tttcaatctg gattttgagc tcgttgtaga
     7801 gctccgccag ctcgtccgcc gtccagcccg catcgtgggc gagttcgagg cggcgcaaag
     7861 cctctcgatt cagcaaatga cccgagctcg actcccccgg gaccacgaga acacggaacc
     7921 ctggagcccc tggcaaaacc gctggcgcaa agaccacgca acattcgcag tactacagcg
     7981 tgggtgccga cgcacgcccc gaatggcgag gctccctgcg gagctgctgg caagatcgtc
     8041 aggcggcccg cggccgagcc acccagcccc cgccgcattg gcgctcccga tactcgggac
     8101 gatattcgct gcgcgcgtcc gcagtcagga gctcacacgg atcgaaccaa tgaatcgtgc
     8161 gagcgcgggc cgttatctcc ggcaatggtc cctcgaactc ggagttctcg ggacaccgcg
     8221 cccgccaaag accaccggcc gtcggtggtt cacgcaacag cagaacctct ttcacgccgc
     8281 cgttgcagtt cgtagacacc acgagaattc tgtcctgcaa gccgctgcct tccggtaact
     8341 cgatcagaac gtcgtggtga aactcgccct gcttgaagtg atcgagcaaa cgaaaagctc
     8401 cccaacgctc gcgcactctc gacagcagct caccgaggac gagacaaccc gcgagctctt
     8461 ccacgatgtc gttgggcatc gggcaagttt gcgcgacgct cgtgacaaag caagacagag
     8521 ccgattcgcg ggcgctcgga cgtccggaac tggctcgccc gagtcgaagt cactcccaaa
     8581 gccggtctag cggcgctcct tcaacacatc ccggatttct gtgagcagca gcacttccgg
     8641 tgagggaggt gccggcgctt tgggcgcctc cggcggcttc ttcttcagct gattgatccc
     8701 cttcaccaac aagaaaatgg cggccgcgac gatcacgaaa tcgaaggtgg cctgcaggaa
     8761 aacgccgtaa ttcagggtga ccggtggttt gccgtccgcg gctgccttca gcaccacctt
     8821 caggtgacta aagtcgaccc cacccacgat caaaccaagc ggaggggtga tcacgtccgc
     8881 caccaaggac gagacgatct ttccaaatgc ggcaccgatc accacgccaa cggcgagatc
     8941 gatgacgttg cccttgatag cgaactctct gaattcttga agaactccca tgaacgctca
     9001 cctttcgcga ggacgtcgag cacgctgccg ctcgagagca agtgcccatc tcctatcatc
     9061 aagtgtcgcc agtccgccga aaaaagagca ccgagagctc gctcgccaat ctgtctatgg
     9121 tggtcgcgac gggtgggtct cgttcggcta tgaatctgcc tgacggacat caatcgagaa
     9181 attcgcgttc tagcgggcct gccgggcgcg gagctagcgg gctattgggc gcgttttcag
     9241 aacccggaat tcgacacgac gattccgctc gcgcgcaccc gcggtagggg aaggatccaa
     9301 gggcgattcg ggccccaaac cttgcgattc caagcgacct gagtcgaccc cgtgctcgat
     9361 caaccaacga acgacggcat ccgctcttcg acgcgacaga gcaaggttca gggctttgga
     9421 gccgatgtca tcggtgtgac cctctactga aacgcgctcg atttctggat gtgcgccgag
     9481 cagttccgcg atcgcagaca gagtttgctc gctactcggg tcgaatcgat cccgacccga
     9541 cgtgaaacga atcggattcg cgacggcaat tcgctcttcc tccagccgca cagagagcgc
     9601 gcagttattg ctttcggggg ccgcgtcggc ctgatccggg acctcgcagt catcggctcg
     9661 ctccacggaa ttggagggat tgctcgtggg ttcgagctct gattcagcgc ctgaatgagc
     9721 aagcatgtcg tgcgtagaga cgcgcctggt gttcgcaggt cgtaacctga attcgagctc
     9781 cgtagcaggc tcgagcctgc gagactcgaa gcccgccacc gggctggtcg ctgcgcgctc
     9841 cgcctcactc aagtcgaagg agacctcgag caccacgcgg aagcgggggg tacccggacc
     9901 gcgcgtgagg ccgggaccga tcgcggctgt gaatgaccag ggtccccgct tgaccgcgcc
     9961 cgcaagtagt agctccaagc tcacacgacg cgagggcgcg atgtgatccg gtgccgcgcc
    10021 aaacagctcg ggaccgaatg accagccttg ggtagctcgc agcgatcgca ctcgtgcgcc
    10081 agcaaagaat gacgtgtgcg tgccgaacag ttcactggct ttgcgctccg aatccgcatt
    10141 cgccaaacgc cgcctaccta cgccagcact ccacgccaat tcacccgcct cgccagaaaa
    10201 gagagcgcta agaccgtacc gcacctgccc cgtgcccgcg acctgcgcgg cactgccggt
    10261 aggcagccag gcttgggcag agaacgccgc cgccaaacct gacgcggcgc cgaacagcac
    10321 gggcaaccga gctccgagcc gtacgtcgcc cagtgcgagg cccgaaggat cgggcgtacc
    10381 gagcgcggct tgcccgtgcc ctccttgtag caacatcggc aagcctaaat cgaagccaag
    10441 gtaacgagac ggcgcgtagg tgccctggag gtggagcgag agagcgcgtt caacctccgg
    10501 cgcatcgccg gtgctccggc gaaatacgag cggcccgtgt gcataactcg cgagtgttct
    10561 caggacgggc gagtccgctt cgaactccgc gttggagccg ctcagcgtgg tactgcccgg
    10621 cgccgagggc tcgaagctgt cgagtgacca ttgcgcgcgt gcgatactcg cgctcagaag
    10681 cacgcctgcg gagcacgcca agacactgct tcggcacatt cgaatctgga acacttactc
    10741 aggaaacggc acgaaggcca ccgggggact gccgtagtgt tctcacggcg aaacgtcgct
    10801 ctgcgcgtaa cactgtgctc gggccgaatg ccgcggcggg agcgtgaacc acaagccttc
    10861 gtgtaactat ttccggaggc ctcgactgaa gttcaacgag aaaaacgccg gtctcccgcc
    10921 accagccaag ggaactcccg cgccagaacc cgcaccatct cctcatccag cccatgggac
    10981 ttggcaggga acaaccaacg cagctccacg ccggcggggg cgggtgcacg caagcgaggc
    11041 tccacctcgg cagcgcagtc gggttggctg catagccaca gcacgcgctt gccgccaccg
    11101 ctcccgaagc gcgcaatcat cggggccgtg aaacgctttg gagagccgtc gatcagcaac
    11161 agatacgaga agaatgtggg ctcctggagc gccagctcga gggcctgatc cacagccggc
    11221 ccgagcgcgg cgagcacgac ccggcctgcc gcgacgtgct tgccaaagcg cgctttgagg
    11281 ctttgcagtc cctcgcgaac acgtccgtgc gtgtcggcca aatcgagtag cccgaagcga
    11341 ccatccgcta acggtttgcc cgcagggcac aacacgaagg cgcggtttcc gctcgcgtga
    11401 cggtagctgc cgcaggtcca ctcgggacga tcgtgtgagc catgcagagc gatcacgatc
    11461 ggtcgcgcct cggtcgcccc caacggaacc gaaacgcgcg cccccgacgg ctccaggttc
    11521 atcaaccaag tcgcgccgga aagcggcgga aggtgccctt cgagctccgg ctggggcttg
    11581 gggcgcgagc aagcggcgaa cccgagcagg agcacgaaca ggccggggcg aacgctaagc
    11641 atgagcccat cttccgtcgc tcataggagt cgttcaatcg cgttcggcag gaatcgcgtg
    11701 gccgcccagg tcaggcgcga actcaccctg tgccacacac gccctcgcgg ctaaaatctc
    11761 tgctggcccg ccgcaatccg ttcgtagtct gcccgcgtga gggggacgaa atgatcgccc
    11821 tgtcgccaga aggcctgagc gcctgacccg ggcgaaaggt cctcgatgag taccggtacc
    11881 cgcttggcct tgagcgagga agtaagcgcg acctcgggca cgaaacggag aaacccaggt
    11941 tgggcgtacg gcgggagccg aggcagctgc cgttccaagt cagccattcg aaagtcattc
    12001 gcaacggcca acgtcagcag cccgaacttg ccgtcgtgac tcggcaaacg cagccccgtg
    12061 cagctcagca tccgaacgcc gggcgtgcga gaaagcgcgt cttccacctc acgcgtggag
    12121 acgttgtggc ccttacatcg aaacgtgtcc cccaagcgat ctacgaagta gtaatagccg
    12181 agccgatctc ggcgcatcag atccccagat cggcagtaga gatcccctgg gcgaaagaca
    12241 tcgcgcgcga tgcgatcggg gcgctccgac tcacccaagt aacccgtgta atcgccggtg
    12301 ctggagtcgg gtcgcacgcg caaacgcagc aacagctccc caagttcgcc gggcttgcac
    12361 tcaatggcga agccgcgagc gtcgcgttcg agctcaccac tttccagatt gacccgggcg
    12421 agacgatagc ctcggatgcg agacaacggg atgtgcccca aactgcca
//
LOCUS       DASTKU010000025        13480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1787633, whole genome shotgun sequence.
ACCESSION   DASTKU010000025 DASTKU010000000
VERSION     DASTKU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13480
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787633"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFQ61_00750"
     CDS             complement(<1..456)
                     /locus_tag="VFQ61_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952994.1"
                     /translation="MEASDESSGAALPLQGSARLSEVLEAGWFEPLLNALGLAWAVFP
                     AHAAVDSADSPEPEPLFQNDAYRTLRGPLREALRKLGESWPGTAGAVREVALGPAGHW
                     RWEVVRIPDQRGGSGWVLQRCTDISELVRERTQRADAAGRLRLLSAHTQG"
     gene            complement(458..2923)
                     /locus_tag="VFQ61_00755"
     CDS             complement(458..2923)
                     /locus_tag="VFQ61_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET9952995.1"
                     /translation="MTLPLPEVLAPAGDAEAMQAAVRAGADAVYFGLQGLNARARATN
                     FSLDGLPSTVAELHRHEVKAYVALNTLVFDSEAELLDQSLAACAAAGVDALIVQDLAV
                     MRRAREVAPEIALHASTQMTCTDAASVNFAAQLGARRVVLARELSLEEIRKIRASSTV
                     ELEVFVHGALCVAYSGQCLTSEAIGGRSANRGACAQACRLPYELYVDGVRKDLGERAY
                     LLSPEDLEASALVPELAALGVRSLKIEGRLKGPEYVAAVTTLYRRAVDALAGAGPAPD
                     QALRTRALQTFSRGSGPGFLKGVDHQRLVEARSCDHRGVLLGRVIGVNTTHARSWLHV
                     ELEAPVARGDGVLIEGGFASEGEAGGRVWAVRQGQTELENVLESGRRVELWLGPETAL
                     KSTCAGRRVFKTNDPRLPSQLERELEAASGSIPLNLLVRARVGAPLEIRGQSARGRWA
                     TVIGEQALTEASGHPATAPQLIEKLDRLGGTPYRLGQVELDLAPNVFVPASLINRLRR
                     QLTDTLGASDQARTECEIPLAPALTWSEVPPPDAGLFALCRSLPQARAALEAGASGVY
                     LDFLELTGLGAAFRALREEFPNHVLGVAPPRVRKPDEQKIDRYLLGLEPDCVLVRGLG
                     ALSEARNSGVTKIGDFSLNVVSRGSAQEVMAQGLRAFTPAFDLDGSQLLALVKAPVGA
                     YAEVVIHHPMPLFHMEHCVYAALLSDGSDFHSCGRPCERHELELRDRSGRQHPVVADV
                     GCRNTVFHGASQSAANLLPSLRAAGVRRFRIELLRETDAETRALVNTYRHLIAGELTP
                     IEAWKRVRAESGYGVVRGSLRVL"
     gene            complement(2920..4401)
                     /locus_tag="VFQ61_00760"
     CDS             complement(2920..4401)
                     /locus_tag="VFQ61_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9952996.1"
                     /translation="MSLLLGSLSHCFHGVIPSVIATASADGTPNVTYVSHVYYIDEKH
                     VALSCQFFNKTRQNVEENPLAAVELYDPLTFDAYRLKISYRRSETQGALFEQMRARLD
                     AIASHTGMHGVFRLLSADIYEVLSVEPVAGFLTPLPEDAPPSLPDPHGPMTELRGLQI
                     ISSRINQAANLDELLNNTLSALEDAFAFDHSMLLLVDETGERLFTVASRGYGESGVGA
                     EVTIGEGLIGIAAAQRQPARIVGMASDLRYGRAIRARARELGHTSTVAEVPLPGLPDA
                     QSQLVLPLVVRERLLGVLAVESTHPLRFEHWHEAFLGVITNQVALALERMTSSEEVEP
                     ERARAAVAALAAPAVAPPLASELAAHESSTALKVPRPAPIELRGGAANAEKHTFWFFP
                     NDDCVFVDGEYLVRNTPGKILWKLLQDFAHQGRREFSNRELRLDQSLGLPALKDNLES
                     RLILLRRRLQDKCPSVKLIPIKRGRFALEVDGPLELVERKGVA"
     gene            complement(4398..4868)
                     /locus_tag="VFQ61_00765"
     CDS             complement(4398..4868)
                     /locus_tag="VFQ61_00765"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9952997.1"
                     /translation="MLPSALIPFVESGLSTIVGTVSSSGQPECMRGIGLVTSDAGSIA
                     RVFLPCDTAGKTVENLRKTRRIAVTVSQPSTHRTVQIKGQLLSLGLASDADRSQIERL
                     FGCFAQELVVIGLPPPILDRVQRWPCWAADFAVGEVFVQTPGPRAGQVLSEADL"
     gene            complement(5039..6637)
                     /locus_tag="VFQ61_00770"
     CDS             complement(5039..6637)
                     /locus_tag="VFQ61_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9952998.1"
                     /translation="MTIRRESLLWTSSVVLALACMDLACSDAADGGKGTGGAPQGEAG
                     QNGEPQAGESSSGGKSSTGGKSSSGGKASGGSATTGGKASTGGSNTAGSGDEPVAGAG
                     SDPDPNPTEKVRFFLPTGEPDNTAQPAVEVDAKGGLHTVYPAYAGGRAYYAYCGANCS
                     GSDDVQVVQFETDATVPNAMLALDAQGRPRVLLSSFNKVFYATCDAACGDASNWTVSE
                     VLDHDGDREVSGEAFALDSNGNPRFLMHTYRAFLGIGQKAPETFLVSCDEDCHSSESW
                     QSSRIAEEIWESSALRFDGNDVPHVATVVRFATGDNAGEKMSAYLSCKGDCTDADNWN
                     GIGFMKPYENETAAVRMPPMVSMALTKAGKPRVVVLGDNAGAKNLIYFECDDEDCTAD
                     HWQGALVSEHEKIDVGFDLALDQQDRPRLVYTLNYNIGLAYCDGAHCGGPEASWDLTP
                     VELGSEMKPDEIFLWDNCTVAAWFLHSPSVALTRDGKPRVGYQARDISGGVSNPDPTK
                     PRCVAGTDMTWTRMAVMPSYQATE"
     gene            7154..9289
                     /gene="fusA"
                     /locus_tag="VFQ61_00775"
     CDS             7154..9289
                     /gene="fusA"
                     /locus_tag="VFQ61_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET9952999.1"
                     /translation="MATRIPLARIRNIGISAHIDSGKTTLTERVLFYTGRIHRIHEVR
                     GKDGVGAKMDSMDLEREKGITIQSAATYCVWKGSKEHLEEHNVNIIDTPGHVDFTIEV
                     ERALRVLDGAILVLDSGKGVQSQSITVDRQMKRYRVPRIAFVNKMDNPGANYERVADM
                     LKEKLGHHPVKLQVPIGAEDKFQGIIDPILGKAFYFEGNDGEVIKEVDVPAEYADAAK
                     RARDEIIEKVAEVDDELAEKFLTEEPISVEELRAAIRRATLALKMTPVMCGSAFRNKG
                     VQLLLDGVVYYLPSPPEVVNEGHDQNKGEEKVVLDSDPEKPFVGLAFKLQQDKYGQLT
                     YFRVYQGRVSTGDTIYNVSNEMRKVRVPRMFRMHSDDREEIQTAEAGDIVAFYGVEAS
                     SGETFTDGKVNITLTSMHVPAAVISLAVAPKDRTSEANFSKALNRFTKEDPTFRVHQD
                     EESQQTIISGMGELHLDIYMERMRREYNCDVIAGKPQVAYRETIQQRSEIAYTHKKQT
                     GGSGQFARVNGFMEPLPQDAVETYEFIDEVVGGAIPREFIPACDKGFKEAMKKGSLIG
                     FPIVGVRCTINDGLAHAVDSSEQAFRTAALMGFRESYSAAKPTILEPIMKVEIEAPVE
                     FQGAVVGQVNQRRGVILETISDENVTITAEVPLNNMFGYSTDLRSATQGKGNYTMEFA
                     KYSPVPKQEQDEMIKKYREKQAKEAAGRK"
     gene            9388..10077
                     /locus_tag="VFQ61_00780"
     CDS             9388..10077
                     /locus_tag="VFQ61_00780"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HET9953000.1"
                     /translation="MRTRKPKSGEFIGADSSEHCPRCALNLAICVCDAVPRVVTRTEI
                     FIIRHAAELRLASNTGRFAALCLPNARILEYAGRSVLDSAALLERADTYLLYHSGGPL
                     TELPLARPPQRLIVLDGTFRQARRMYKRIAELRRLPELALPPPAVAPARLRQPPTREG
                     MSTLEAIAHALSLLEGPELGKPLHDLQSELVRRFDALGGRRRVPPLPAAQAPEDPGPE
                     VGGSSASFSSR"
     gene            complement(10014..11306)
                     /locus_tag="VFQ61_00785"
     CDS             complement(10014..11306)
                     /locus_tag="VFQ61_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9953001.1"
                     /translation="MSLPPPTTEYPAERVRKILLVILALGISLLFLWMIRDFLVALLF
                     AALAAGMSYPYYRKLSIRLKGRNPLAASITLGVLLLLVIIPVTAFGALVAVQAAEIGE
                     AAGPWVKDQIHQASGEDFLTRFPELSRLSPYRDQILAKVGELSASLGSMAVTLVTEAA
                     RQTATFFLLLFVMLYATFFFLLTGRATLRKILYYLPLPPEDEDRIVGRFVSVARATLK
                     GTFVIGFAQGALGGLGFWVADIQGAALWATMMGVLAIVPGLGPVMVWLPAVIYLFVMQ
                     RFGAAFGLGAWCIGVVGTVDNFLRPWLVGKDTKMPDLLILLATLGGIVMFGALGFVIG
                     PIIAALFVTIWDIYGDVFRNVLPEPPPLSTIPPVPSQALAESIRSFQSRVTGAPAASG
                     AAPLGKRESAARVDQSEAQRDEKDADEPPTSGPGSSGA"
     gene            11354..12721
                     /locus_tag="VFQ61_00790"
     CDS             11354..12721
                     /locus_tag="VFQ61_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953002.1"
                     /translation="MPRRSWAARRPFPCQECRAEQSLSEKPTAAANPDARATSARRMG
                     TRARPILAIFSPISCALGWLGVLGAVGALACSEAAPQGFAPYAGDAYPNRRARFEANG
                     HTLGYVANRFSDSITVLDLDTMQTLGTAPVGRDPADTDGPRHILLDEPHGLAYVALTY
                     PLEVRSPHVVMEGAPERASYVQALALDDLRVMGEVRLDTSADEMAFSPAAGSLVVSHR
                     DPLKGLSLTPTPLVWIDEPWRMSDNTVTQRRLSPCVAPGSLAWDRSSALVYVACTGED
                     KLGVIDAQTTPRVLEFVASGMDVVNQPYAVVADESGPRLLVSNQVAQKVAVFEVGSGP
                     DSGSTQSAARQIGSITVSGVPGFSAFLGREALIIPQRDPAGVLRVDAQSLEIQATSQL
                     SSTDCENPSEARATEDGRLFLVCEGDHYSPGAVLKLDPNTLATEAKVNVGLYPDRLAV
                     RPFSR"
     gene            13275..>13480
                     /locus_tag="VFQ61_00795"
     CDS             13275..>13480
                     /locus_tag="VFQ61_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953003.1"
                     /translation="MRHDAGFIWRTFGLVSCASTLSLAACSGDSTSGGTAGTGTVAPG
                     SSGANAGGNAGSGAGGNASGGASAP"
BASE COUNT         2579 a         4398 c         4171 g         2332 t
ORIGIN      
        1 gccctgcgtg tgcgccgaca acaggcgcag cctcccggcg gcgtcggccc gctgcgtgcg
       61 ctcccgcacc agctccgaaa tgtcggtgca gcgctgcagc acccagccac ttccacctcg
      121 ttgatccggg atgcggacaa cttcccaccg ccaatgtcca gctgggccga gcgccacctc
      181 acgcacggcg ccggccgtgc cgggccaact ctctcccaac ttgcgtaaag cctcccggag
      241 cggaccgcgc aaagtcctat aggcatcatt ctggaacagc ggctcgggct ccggagaatc
      301 ggctgaatct acggcagcgt gcgccggaaa cacggcccag gccaagccga gggcgttcag
      361 caaaggctcg aaccatcctg cttccaagac ttccgacaat cgagccgagc cttgcagcgg
      421 gagggcggcg ccgctcgatt cgtcggacgc ttccaagtta aagcacccgt agtgaaccgc
      481 gcaccacgcc gtagcccgac tcggctcgca cgcgtttcca agcctcgatg ggagtgagct
      541 cacccgcaat caagtggcgg taggtattga cgagcgctcg ggtctcggcg tccgtctcgc
      601 ggagcagctc gattcgaaag cgccgaaccc ccgccgccct cagactcggc agcaggttcg
      661 ccgcgctctg ggacgcgccg tgaaataccg tatttcgaca gccgacgtcc gccaccacgg
      721 ggtgttgccg gcccgagcga tcgcgcagct cgagctcgtg ccgctcgcag gggcgcccgc
      781 agctgtggaa atccgagccg tccgagagca acgccgcgta cacgcagtgt tccatgtgga
      841 acaacggcat agggtgatga atcacgacct ccgcataggc gcccaccggc gctttgacca
      901 aggccaatag ctgtgagcca tctagatcga acgccggagt aaaggccctc agcccctgag
      961 ccatgacctc ctgggcactc ccgcgcgaca cgacattcag agaaaagtct ccaatcttgg
     1021 tcacccccga gttgcgcgcc tcggacaacg cgccgaggcc gcgcaccagt acacaatccg
     1081 gttcgagtcc gagcaggtag cggtcgatct tctgctcgtc gggctttcgc acacgcggtg
     1141 gcgccacccc caaaacgtgg ttcggaaact cctcgcgcaa ggctcggaac gccgcaccta
     1201 ggccggtcag ttccaggaag tccaagtaaa cgccgctcgc gcccgcctcg agtgccgccc
     1261 gcgcttgagg cagactccgg cacagcgcaa acaagcccgc atccggcggc ggaacctcgg
     1321 accaggtcaa ggcgggagcc agaggtattt cgcactcggt gcgggcctga tcgctggccc
     1381 caagcgtgtc agtcagttgc cgccgcagac ggttgatcag cgacgccggc acgaacacat
     1441 tcggagccaa gtccaactcg acttgcccca agcggtaagg cgtgccgccc aatcggtcga
     1501 gtttctcgat caactgcggc gcggtcgccg ggtggcccga agcctcggtc aatgcctgtt
     1561 caccaattac cgtcgcccat cgcccgcgcg cggattgacc ccgaatttcg agcggggcgc
     1621 cgacgcgagc gcgcaccagc aagttcaaag gtatcgaccc cgacgcggcc tcgagctcgc
     1681 gctcgagctg actcggcagc ctcggatcat tggtcttgaa cacccgtcgc cccgcgcacg
     1741 tgctcttcag cgcagtttcg gggcctaacc acagctcgac gcgccggcca ctctcgagga
     1801 cgttttcgag ctcggtctgc ccttggcgaa cggcccaaac gcggccgccc gcttcacctt
     1861 cggaggcaaa accaccctcg atcagcacgc cgtccccgcg cgcgacagga gcttcgagct
     1921 ctacgtggag ccagctccgc gcatgggtgg tgttcacgcc gatcactcgt cccaggagca
     1981 cgccgcgatg atcgcaggat cgagcctcca ccaagcgttg gtggtcgacg cccttcaaga
     2041 aacccggccc tgagccgcgg gagaaggttt gcagggctcg cgtgcgtaga gcttggtcag
     2101 gcgctgggcc cgcgcccgcg agcgcgtcca cggcgcggcg gtacaacgta gtgaccgcgg
     2161 ccacgtactc ggggcctttc agtcgccctt cgatctttag agaacgaact ccgagcgctg
     2221 caagttcggg cacgagcgcg ctggcctcta gatcctcggg cgagagcagg taagcgcgct
     2281 cgcccaggtc tttgcgcacg ccgtccacgt agagttcgta cggtaggcgg caggcctggg
     2341 cgcatgcacc gcgatttgca ctgcgacccc cgatcgcctc gctcgtcaag cactgccccg
     2401 aatacgcgac gcacagcgcg ccatgcacga agacctcgag ttcgaccgtg ctcgaggctc
     2461 gaatcttacg gatctcctcg aggctcaact cgcgcgcgag caccacccgg cgggccccga
     2521 gctgggccgc gaaattcacc gacgccgcgt ccgtgcacgt catctgcgtg gaggcgtgaa
     2581 gggcgatctc gggcgccact tcgcgcgcac gccgcatgac cgccagatct tgaacaatca
     2641 atgcgtcgac accggccgcc gcacaagccg cgaggctctg atcgagcaac tcggcctcgc
     2701 tgtcgaacac caaggtattc agcgcgacgt aagccttgac ctcgtgtcga tgcagctccg
     2761 caacggtgga cggtaagccg tccaacgaga agttcgtggc ccgcgcgcgg gcgttcaggc
     2821 cttgcaagcc aaagtagacc gcgtccgctc cggcgcgcac ggccgcctgc atggcctccg
     2881 cgtcccccgc gggcgccagc acctcaggca acggcagggt cacgcgacgc cttttcgctc
     2941 cacgagttcg agcgggccgt ccacttccag cgcgaaacgg cctcgtttga tggggatgag
     3001 cttcacgctc ggacatttgt cctgcaggcg ccggcgcagc aaaatcaggc ggctctcgag
     3061 gttgtccttc aatgctggaa gccccagact ttgatccaag cggagctcgc ggttcgagaa
     3121 ctcacggcgc ccctggtgcg caaaatcttg cagcagcttc cacagaattt tgcccggggt
     3181 gtttcgcacg aggtactccc cgtccacgaa aacacaatca tcgttcggaa aaaaccaaaa
     3241 cgtgtgtttc tcggcattgg cagccccgcc gcgcaactca atcggggcgg gtcgcggcac
     3301 cttcagcgcc gtagacgact cgtgagccgc gagctccgac gcgagtggcg gggctacagc
     3361 cggtgcggcc aacgctgcga cggcggcgcg cgctcgctcg ggctctactt cctcgctcga
     3421 ggtcatgcgt tcgagcgcca gagccacttg gttggtgatg acgccgagaa acgcttcgtg
     3481 ccagtgctcg aagcgcagcg ggtgcgtgct ctcgacggcc aagaccccga gcagccgttc
     3541 gcgcaccacc agcggcagca ccagctggct ctgcgcatcc ggaagcccgg gcaaaggcac
     3601 ctccgccacg gtggaggtat gcccgagttc gcgcgcccgt gcgcgaatcg cccgtccgta
     3661 acgtagatcc gaagccatgc cgacgatgcg cgcgggttgc ctttgcgccg ctgcgatgcc
     3721 gatcaagcct tcgccgatcg tcacttcggc gcccacgccg ctctccccgt aaccccggct
     3781 cgccacggtg aacaggcgct cgcccgtctc gtcgaccaag agcaacatcg agtgatcgaa
     3841 ggcaaaggca tcctctagcg cgctcagggt gttattgagt aactcatcca agttcgcagc
     3901 ttggttgatg cgtgaggaga tgatctgcaa accgcgcagt tccgtcattg gcccgtgtgg
     3961 atcaggcaac gagggcggcg cgtcctccgg caaaggagtc aagaatcccg cgaccggctc
     4021 cacactcaac acctcgtata tgtcggccga caataagcgg aatacgccgt gcatgccggt
     4081 atgcgaggcg atcgcgtcga gccgtgcgcg catctgttcg aataacgctc cttgcgtctc
     4141 cgaacgacga taagagatct tgagtctgta cgcgtcgaac gtgagcgggt cgtatagctc
     4201 cacggccgcc agcgggttct cctccacgtt ttgacgagtc ttgttgaaaa actggcagga
     4261 aagcgcgacg tgcttctcgt cgatgtaata gacgtgactc acgtacgtga cgttcggcgt
     4321 gccatccgcg ctggccgtcg cgatcacact tggaatgacg ccgtggaaac agtgcgagag
     4381 gctcccgagc agcaagctca taggtccgcc tccgacagca cctgaccggc gcgcggcccg
     4441 ggcgtttgca cgaacacctc cccgaccgcg aagtccgccg cccagcaggg ccaccgctgc
     4501 acgcgatcga gaatgggtgg tggcaaaccg atcaccacca actcctgggc aaaacagcca
     4561 aagagccgct cgatttgaga ccggtccgcg tccgaggcga ggccgaggct caggagctgg
     4621 cccttgattt gcaccgtacg gtgcgtactg ggttggctca cggtaacggc aatgcgccgt
     4681 gttttccgca gattttcgac ggtctttccg gccgtgtcac acggcaggaa cacgcgcgcg
     4741 atgctccccg cgtcgctcgt cacgagccca attccgcgca tacactccgg ctgaccggag
     4801 ctgctcacgg tacccacgat tgtagatagg ccgctctcga cgaaagggat cagcgcgctg
     4861 ggcagcatcg acgcggcgaa gatagctgaa gtcgaacgcc gcgtcaggtc gccaggcgcg
     4921 cacacctccc ctgtaccgct ctgccccgcc ctcggaactc gcgcgagccg ggccggccgc
     4981 gacgaacggg ccggccctcg atgcttcgga tctactcgaa ggtgttactt cagggacgtc
     5041 attctgtggc ctggtacgaa ggcatgaccg ccatgcgcgt ccaggtcata tcggtgcccg
     5101 ccacgcagcg gggcttggtg gggtcggggt tgctcacacc gccgctgatg tcgcgcgctt
     5161 gataaccgac gcgcggcttt ccatctcggg tcagtgcgac cgaaggagag tgcaggaacc
     5221 aagcggcaac ggtgcagttg tcccaaagga agatctcgtc gggtttcatt tccgagccga
     5281 gttctacggg cgtcagatcc cagctcgctt cgggaccacc gcaatgggct ccgtcgcagt
     5341 aggccaatcc gatgttgtag ttgagcgtgt ataccagacg ggggcgatct tgctgatcga
     5401 gcgccagatc gaagccgacg tcgatttttt cgtgctcgct aaccagggca ccctgccagt
     5461 gatccgcggt gcaatcctcg tcgtcgcatt cgaagtaaat caagttcttg gcgccagcgt
     5521 tgtcgcccag caccactacg cgcggtttgc cggccttcgt gagcgccatc gacaccatcg
     5581 gcggcatgcg caccgccgcc gtttcgtttt cgtacggttt catgaagccg atgccgttcc
     5641 agttgtcggc gtcggtgcag tctcccttac aggagagata cgccgacatc ttctcgcccg
     5701 cattgtcgcc cgtggcaaat ctcaccacgg ttgcaacgtg cggaacgtcg ttcccatcga
     5761 atcgcagggc cgagctctcc cagatttctt ccgcgatccg cgagctctgc cagctctcgg
     5821 acgagtggca gtcctcgtca cagctcacga ggaacgtctc cggtgccttc tgtccgatgc
     5881 caaggaaggc tcggtaggtg tgcatcaaga agcgtggatt gccgttgctg tcgagggcaa
     5941 aggcctcacc gctcacctcg cggtcaccgt cgtgatcgag gacctcgctc accgtccagt
     6001 tcgaagcatc cccgcaggcc gcgtcgcagg ttgcgtaaaa aaccttgttg aaagaggaga
     6061 gcagcacgcg cggtcggcct tgcgcatcga gcgcgagcat cgcgttcggc acggtggcgt
     6121 cggtctcgaa ttggacgacc tgcacgtcgt cactgccgct acagtttgcc ccgcaatatg
     6181 cgtagtaggc gcggccccca gcgtacgcgg gatacacggt gtggagtccg cctttggcgt
     6241 ctacctccac ggcgggctgc gcggtgttgt cgggttcacc ggtgggtaag aagaagcgca
     6301 ccttctccgt gggattggga tccgggtcag agcccgcacc ggcaacgggt tcatcgccgc
     6361 tgcccgccgt gttgcttcca cccgtgctgg ctttgccgcc ggtggtcgcg ctaccaccac
     6421 tggctttgcc gccgctgcta ctcttgccac cggtactgct cttgccgccg ctggagcttt
     6481 caccagcctg cggctcgccg ttctgccctg cctcaccctg aggcgctccg cctgtgcctt
     6541 tgccgccatc cgcggcgtcg ctgcaggcca aatccatgca agccaaagct aagactacgc
     6601 tactcgtcca caacaaggat tcacgtcgga tcgtcatcgt cgtgaacatc aatgtggggt
     6661 acttccgggc acgcgcttcc taaatggtcg ctgcgtgaat cctacgagtt tgctaaacgc
     6721 ccccgcagct cgcgctcgca tgggattcgt ttcgacgaat cacgcggctc actcacccgg
     6781 aggagtattc acgtgcgctc cgtcgctcgc tgatcgaggc tcagaaggtg aatgacaggg
     6841 tgtgcgcgtg cgggtcgccg tgccgcgctc gttccgctct acagcgtcag ctccgcctct
     6901 cgccagccca accacacccg ccgggcggcc tagcctcggc ctggcgaatt ccaaccgggc
     6961 tacaatattg gtgcgagatt gggcgtgaat cccattccaa aagcgagcca aacgcggctc
     7021 cttctgctag atggaggccc gccgggcggc ctaggtaggg cggctccgag cgcctcccgt
     7081 ccaaacgagc ttccattctg gcctcggttt ggatactggc cgcttcctct aactccacac
     7141 cgaagaaaaa cacgtggcaa ctcgcatccc cctcgctcgc attcgcaaca tcggtatctc
     7201 ggctcacatc gactcgggca aaacgacgct gaccgagcgt gtgctcttct acaccggtcg
     7261 tattcaccgg atccacgaag tgcgcggtaa ggacggcgtt ggcgcgaaga tggactcgat
     7321 ggacttggag cgtgagaaag gcatcacgat ccagtccgcc gccacgtact gcgtgtggaa
     7381 gggctcgaag gagcatctcg aggagcacaa cgtcaacatc atcgacacgc ccgggcacgt
     7441 agacttcacg atcgaggtgg agcgtgcctt gcgcgtgctc gacggcgcga tcctggtgct
     7501 cgactccggc aagggcgtgc agagccagtc gatcaccgtg gatcggcaga tgaagcgcta
     7561 ccgcgtcccg cgcatcgcgt tcgtcaacaa gatggacaac ccgggcgcga actacgagcg
     7621 cgtcgccgac atgctcaagg agaagctcgg ccaccatccc gtcaagctgc aggttccgat
     7681 cggcgccgag gacaagttcc aaggcattat cgacccgatt ctgggcaagg ccttctactt
     7741 cgagggcaat gacggtgagg tcatcaaaga ggtggacgtg cccgccgagt acgcggacgc
     7801 ggccaagcgc gcgcgggacg agatcatcga gaaggtggcc gaggtcgacg acgagctcgc
     7861 cgagaagttc ttgacggaag agccgatcag cgtcgaggag ctgcgcgctg caattcgccg
     7921 cgcgactctg gcgttgaaga tgacgccggt gatgtgcggc tccgcgttcc gcaacaaggg
     7981 tgtgcagctg ctgctggacg gcgtggtgta ctacctgccg agcccgcccg aagtcgtgaa
     8041 cgaaggtcac gatcagaaca agggcgagga gaaggtggtt ctggactccg atccggagaa
     8101 gccgttcgtc ggcttggcgt tcaagctaca gcaagacaag tacggtcagc tcacttactt
     8161 ccgcgtctat caaggccgcg tctccaccgg cgacacgatc tacaacgtga gcaacgagat
     8221 gcgtaaggtg cgcgtgccgc gcatgttccg catgcactcg gatgaccgtg aggaaatcca
     8281 gacggcagag gccggcgaca tcgtggcctt ctacggcgtc gaggcgagct ccggcgaaac
     8341 cttcaccgac ggcaaggtca acatcacgct gacctccatg cacgtgccgg ccgcggtgat
     8401 ctcgctcgcg gttgctccga aggaccgcac ctcggaggcc aatttctcga aggctctgaa
     8461 ccgcttcacc aaggaagatc cgaccttccg cgtgcaccag gacgaagagt cacaacagac
     8521 catcatcagc ggcatgggcg agctgcacct cgacatctac atggagcgca tgcgtcgcga
     8581 gtacaactgc gacgtgatcg cgggcaagcc tcaggtggcc taccgcgaga ccatccaaca
     8641 gcgctccgag atcgcttaca cgcacaagaa acagaccggc ggttctggtc agttcgcgcg
     8701 cgtcaacggc ttcatggagc cgctgcctca agacgcggtg gagacctacg agttcatcga
     8761 cgaggtggtg ggcggcgcga ttccgcgtga gttcatcccc gcttgtgaca agggcttcaa
     8821 ggaggccatg aagaagggct ccttgattgg gttcccaatc gtcggcgtgc gctgcacgat
     8881 caacgacggc ttggcccacg cggtggactc ttccgagcag gcgttccgca ccgccgcgct
     8941 gatgggcttc cgcgagtctt actcggcggc caagcccacc atcctcgagc ccatcatgaa
     9001 ggtggagatc gaggccccgg tggagttcca gggtgcagtc gtgggtcagg tgaaccagcg
     9061 ccgcggcgtc atcctcgaga cgatcagcga cgagaacgtg accatcacgg ccgaggtgcc
     9121 actgaacaac atgttcggtt actcgacgga tctgcgctcc gcgacgcagg gcaaaggcaa
     9181 ctacaccatg gagttcgcca agtactcgcc cgtgccgaag caggagcagg acgagatgat
     9241 caagaagtac cgcgagaagc aggccaagga agccgcgggc cgcaagtaat cgcgactcgt
     9301 cgagccacgc ccccgttccc gcacgagctc ccgagctcgg cgggtccggg ggttcgcttt
     9361 ttgtggcagc atcagcggtc ccgatcgatg cgaactcgta agcccaaaag cggtgagttc
     9421 attggcgcag acagctcgga gcattgcccg cgctgcgccc tgaacctcgc catctgtgtg
     9481 tgtgacgcgg tgccgcgcgt ggtgacgcgc accgagatct tcatcattcg tcatgccgcg
     9541 gagttgcgct tggccagcaa caccgggcgt ttcgcggcgc tttgcttacc gaatgcgcgg
     9601 atcctggagt acgcgggtcg cagcgtgctc gacagcgcgg cgttgctcga gcgcgcggat
     9661 acgtatttgc tctatcactc gggagggccg ctcaccgagc tgccgctggc gcgcccaccg
     9721 caacgcctga tcgtgctgga tgggaccttt cgccaagctc gacgcatgta caagcgaatc
     9781 gcggagctgc gtaggctgcc ggagttggcg ctaccgccgc ctgccgtcgc gccggcgcgg
     9841 ctgcgccagc ctcccactcg tgaggggatg tccacgctgg aggccattgc gcacgcgctg
     9901 agcctgctcg agggcccgga gctgggcaaa cccctgcacg acctgcaatc cgagttggtc
     9961 cgacgcttcg atgcgctcgg cggacgcagg cgggttcccc ctttgcccgc cgctcaagcc
    10021 cccgaagatc ccgggcccga ggtcggcggc tcatcggcat ccttttcatc gcgctgagcc
    10081 tccgattgat ccactcgcgc tgccgactct cgcttgccga gcggagcagc gccgctcgcg
    10141 gcgggagccc ccgtaactcg ggactggaac gatcgaatgg actctgccaa cgcttgcgaa
    10201 ggcaccgggg gaatggtcga aagcggaggc ggttcgggca ggacgttgcg aaacacgtct
    10261 ccgtaaatgt cccagatggt cacgaacaaa gccgcgatga tgggaccgat cacgaagccg
    10321 agcgccccga acatcacgat gccacccaac gtggcgagca gaatcaagag atcgggcatc
    10381 ttggtgtcct tcccgaccag ccatggccgc aggaagttgt ccaccgtacc caccacgccg
    10441 atgcaccaag cacccaaacc gaaagccgcg ccgaagcgct gcatgacgaa caggtagatc
    10501 accgccggta accaaaccat cacggggcca aggccgggca cgatcgcgag cacacccatc
    10561 atcgtcgccc acagtgccgc gccttgaatg tccgccaccc agaagccaag tccgcccagt
    10621 gcgccctgag cgaatccgat cacgaatgta cccttgaggg tagcgcgcgc gaccgacacg
    10681 aaacggccaa cgatcctgtc ttcgtcctcg ggcggcaggg gaaggtaata tagaatcttg
    10741 cgtagcgttg cgcgcccggt cagcaagaag aagaacgtgg cgtacagcat cacgaacagc
    10801 agtaaaaaga acgtggcggt ctggcgcgcg gcttcggtca ccaaagtgac ggccatgctg
    10861 ccgagcgagg cgctcaattc acctaccttg gccaaaatct gatcacggta cgggctcagg
    10921 cgcgaaagct cgggaaaccg agtcaggaag tcttcacccg acgcttgatg aatctgatct
    10981 ttcacccatg gtcccgccgc ctcaccgatt tctgccgcct gcaccgcgac gagcgcaccg
    11041 aaggcagtaa ccgggatgat caccagtaga agcaacaccc cgagcgtgat cgaggccgcg
    11101 agtggattgc gccccttcag gcggatagaa agcttccggt agtacgggta ggacatgccc
    11161 gccgcgagtg ccgcgaacaa cagcgcaacc agaaagtcac ggatcatcca caagaacagc
    11221 aggctgatcc cgagcgcgag aatcacgagc aggatcttgc gcacgcgttc cgcgggatac
    11281 tcggtcgttg ggggcggcag ggacattggc cgcagcctat cgcacgcgca ggcggatggg
    11341 ctaagctccg cacgtgccgc gtcgaagctg ggccgcccgc cggcccttcc cttgtcagga
    11401 gtgccgggcc gagcaatctc tgagcgaaaa gcctacggcc gcggcgaatc cagacgcgcg
    11461 ggcgacgagc gcgcggagaa tgggcacgcg ggcgcgtccg attctggcaa ttttctcacc
    11521 aatttcttgc gccctcggct ggctgggggt gctcggagcg gtgggagctt tggcctgtag
    11581 cgaagccgct cctcagggtt ttgcgccgta tgccggggat gcttatccca accgccgggc
    11641 ccgattcgag gccaatgggc ataccctcgg ttatgtggcg aatcgttttt ccgattcgat
    11701 caccgtgctc gacctcgaca cgatgcaaac gctcgggacc gccccggtcg ggcgcgatcc
    11761 cgccgataca gacggtccac ggcacatcct gctcgacgaa ccccacggcc tggcatatgt
    11821 ggctctcacc tacccgctcg aggtacggag tcctcatgtc gtcatggaag gagctcccga
    11881 gcgggctagt tacgtccagg cgctggcgct cgacgaccta cgcgtgatgg gcgaggtccg
    11941 tttggacacc agcgcggacg agatggcgtt ttcgccagcg gcgggcagcc tcgtggtttc
    12001 gcatcgtgac ccgttgaagg gcctcagcct cacgccgacc ccattggtgt ggatcgatga
    12061 gccgtggcgg atgagcgaca atacggtgac acaacgaaga ctttcgcctt gcgtcgcacc
    12121 gggcagcctg gcttgggacc gcagctcagc tctggtttac gtggcctgta ccggcgaaga
    12181 caagttggga gtgattgatg cccagacaac gccgcgtgta ttggagtttg tggcgtctgg
    12241 aatggatgtg gtgaatcaac cgtacgccgt tgtcgccgac gagagcgggc cacgcttgct
    12301 cgtttcgaac caggtcgctc aaaaagtggc cgtcttcgag gtcgggtctg gcccagactc
    12361 tggctcgacg cagtccgcag cccgccaaat cggttcaatt accgtgagcg gcgtgccagg
    12421 tttctctgcg ttcctgggcc gcgaagcact cattatcccc cagcgagacc cagctggggt
    12481 gctgcgggtc gacgctcaaa gccttgaaat tcaggctact tcgcagctga gttcgacgga
    12541 ctgcgagaac cctagcgagg cgcgtgccac cgaagatggg cggttgttcc tagtctgcga
    12601 aggcgaccac tacagccctg gagccgttct gaagctggac cccaatacgc tcgcaaccga
    12661 ggcaaaagtg aacgtcgggc tttaccccga tcggctagcg gtgcggccct tttcgcgatg
    12721 atggtcggtg caaattcgga atctcgcgag tctgcaggca tgagcacgcc caaacgggcc
    12781 aattaagttg gtgacgagaa cgtaaaaaga atgggccgat taggcctgct tcgctgtcga
    12841 acgattggct atccaatcat cgagtcgtga tagcgggagt ctcggtcgaa aaaaattcca
    12901 ccgccttctc gacgcgaatc ttgcgtgcta ctaagtgcga ctatcagtcg cgacttgggc
    12961 catcgtccaa gcgtcgacct aaatcagtgg gcggtttttc cgatcgcatt tcgaagagga
    13021 aacaggcagt ttgctgctca atcctcgcgg aagctaacca acacacaccg gaagcgtacg
    13081 agttctgcgg gcgtgatctc agaaattaga ggccgtattc tggacaccaa gccgccctgg
    13141 tcagccgaga gctgatagct agccgagagc tgatagctag ccgagccagc agaagcaggg
    13201 tgccgggaga gccaagcggc aaacatctct gaggcgcacg agccgccccc tgaagcgaag
    13261 caccacgagg tatcatgcga cacgacgcgg gtttcatttg gcggacattt ggcttggttt
    13321 cgtgcgcgag cacgctatcc ctggctgcct gcagtggaga tagtacgagc ggcggcactg
    13381 ccggcaccgg caccgtcgcg ccgggctcca gcggagccaa tgcgggaggt aacgcgggca
    13441 gcggggcggg tggcaacgcc agtggcggag cgtcggcacc
//
LOCUS       DASTKU010000026        12309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_896454, whole genome shotgun sequence.
ACCESSION   DASTKU010000026 DASTKU010000000
VERSION     DASTKU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12309
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896454"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..940)
                     /locus_tag="VFQ61_00800"
     CDS             complement(227..940)
                     /locus_tag="VFQ61_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953004.1"
                     /translation="MKKRVWSEALRRVRASALVWCTLVPLTWPQVGCASAGASAQPDQ
                     TLQAYARAVQGGRAREAYSLLSKQAQKNIPFESFERILKENPEELSEFAKSLQRPASP
                     PLVTATVTPSNGPPLLLVYENGAWRVDASSVDLYSQATPERAVAAFVRAYENQRYDVL
                     LRFVPDAQKHELTPASLKQAWTVDQKEDVARMMQALKAALPSARFELLGDRATLAYGA
                     GGSLELLREHGAWKVEDLK"
     gene            1202..3610
                     /gene="lon"
                     /locus_tag="VFQ61_00805"
     CDS             1202..3610
                     /gene="lon"
                     /locus_tag="VFQ61_00805"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET9953005.1"
                     /translation="MCPVAGTRPERIAILPLRNSVLFPMSVVPINVGRQRSVRLVEEL
                     LGKDSALVGVLTQRNADTVEPTFEDLYGIGTLARVVKVIRLGPSNYSVVLNGLGRFKL
                     LEPQGLEPYMRADIERVPELGQTNAELTRLGQKLRENTREVLGLLPNLPKETASILDN
                     VREPGALADLIASNFPEEHASIHVRQRILEAFDVKARVELVLGMVGRQLEVLKVRDEI
                     SSQVQNELSRSQRDYVLRQQMRSIREELGESAEDDEIEQLRERFSRAELPAEAERAAR
                     KQLARLSNMQPQSAEYQVTRNYVEWLADLPWNRRTPDRVDVKEVRRCLDEDHYGLETV
                     KKRIVEYCAIRQLRKDKRGPILLFIGPPGVGKTSLGRSIARAMGRRYGRISLGGVRDE
                     AEIRGHRRTYVGALPGRIIQALKKVGCRNPVLVLDEVDKMGVDLRGDPAAALLEVLDP
                     AQNDSFVDHYIDLPFDLSEVMFIATANYRGNIPEALKDRMEVIDVLGYTRTEKRAIAE
                     QFLVKKQLKENGLKDEQLSFTREGIETLVDFYTREAGVRSLEREIAAVCRDATVSFAE
                     GRRSGHLRVTPEVVREILGPEKHRPEQTERKLAPGVAIGLGVASGGGDLLIVEATRMP
                     GKGEVVITGSMRGVMRESATTAVSFVRSRADRLRLDPEWLRKIDLHLHVPRAAAVRDA
                     ASAGVAMFVAVVSLLLGVSARSDVAMTGELTLRGAILPVTGIKEQVLAAHRSGIRRVL
                     IPARNERDFADVPDEVRQDIEVHFVNRLDEVLALVLEQPSADEASARDESAGDGAQGD
                     EASP"
     gene            3746..6058
                     /locus_tag="VFQ61_00810"
     CDS             3746..6058
                     /locus_tag="VFQ61_00810"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9953006.1"
                     /translation="MRIKSWLPPFEQRALVLSTLLAAALGFAPQLAGPGYDSALIAGA
                     LGPGWCGVVASWSVLQSEEPSGRTAYGRGVRIGLCSWATAFLVCLLHGLRVGMCDPVE
                     GLWLWLLGPGFGSVLGGVSGALAGLWIRAHASSWSVRWSRSIGAGLGLAGPLLGISVS
                     LLRFYTSPMVFAYDPYFGYFSGPLYDTVIGSLWPLVTYRAGTLASLLVLALATSLVTW
                     PANGAPRWNKPSGLQVLGLVLFTLLSVGISAAGTRLGHASTARSIQQELGRESQFGRC
                     RIVHSRGVTERDAQRLGRECHAHLRQIERFFGAAGPATVTVYLFANDQEKGRLMGAAH
                     TYIAKPWRHEVYVQAAGFPHPVLGHELAHVVSGSFGQGPFEVAGKLGGLWPDPGRIEG
                     VATAASPDENDALTLEEWAAAMQRLELLPPLGSLFQFGFFGRNAAQAYTVSGAFVRFL
                     RASYGASAVRRWYGGEGLPSITGKSLADLEQRWRAELRELTLSDAALAAARARFQRPS
                     FFDRACPRVIDRELGEAQGQLVLADFGGARRGFEGVLRLDPHDSAARLGLAACSARSG
                     QIEAAARSYRALQRATDAAPWARLQALEAEADLALRRGNLDEARALYAELAQRIVDED
                     RLRSLDVKRLAEPGLAQRAIVALLIGDELGPAWDSAAPLLGEWSASNASDGLADYLLA
                     KNLFGRNRARDAQRYIERALTRALPEPRVHDEALRLQLLVGCASNDPERARPAYTSLL
                     ARPITRAKRVALERIAERCGIESAPNPVAP"
     gene            complement(6076..6897)
                     /locus_tag="VFQ61_00815"
     CDS             complement(6076..6897)
                     /locus_tag="VFQ61_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9953007.1"
                     /translation="MARSRSGTAWQLAWLLPVALIWLFPYAWMVLTSLKTLPEIVQAP
                     HAWLPRSWNGQAYERALASMPIARYLLNTAAMAIGIAVLQLLLAIPAGYALAKLRFRG
                     RSLCLVLILATLLLPAQLRFVPTFMLFAKLGLSNTLASLILPFGVSALGTFLVRQALL
                     SVPDAVIEAARIDGSSELRIVYGLLPPLIVPTLVAVFLFSFVYHWNDYFWPLVMTTDD
                     RARTLPVGVALLREQGTGVRWHIVMAGNVILSAPVLALFAVAQRHLLRAVLGSSS"
     gene            complement(6909..7775)
                     /locus_tag="VFQ61_00820"
     CDS             complement(6909..7775)
                     /locus_tag="VFQ61_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET9953008.1"
                     /translation="MRPAHRFDPAWLLGPTLAFLALFYFYPLLLAAYYSLLDWDLLTP
                     ARYVGFSNYRALLSSGSFATVLGTTLLISVLSVAGSMLAGLSLALALNRPGKLVAFIR
                     SSVFSAYVVSWVSVGLLWLWLLDADAGLVSALIQRLAPGSKGLLANVTTAPLALSAVL
                     VWKTAGYAMVVFQSGLGSIPQSVLEAAELDGAGRVARLAHVVLPLLGPTTAFVAITSF
                     ILSFQAFDVVRVMTQGGPVRSTTVFVYSIYEQMFMDLRVGRASAEVMLFFVILLVLTA
                     LQLRISRRREAL"
     gene            complement(7777..9051)
                     /locus_tag="VFQ61_00825"
     CDS             complement(7777..9051)
                     /locus_tag="VFQ61_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9953009.1"
                     /translation="MPQTIRHPFRVGRRAFVTGTLGLACESNSKLRAGRHRIALWFTY
                     GGKNREVLEALVAKFNASQGEIFVEGVFQGDYFEGLAKLRVALAVGDPPALSHVVGEV
                     IPYLAEAGALQRLDAFTSLATLRLHPSLGQAGSFIGGAERPLWALPFNRSTPIAYLNG
                     QVFAEAGLHAPRTWPELVETARALTLRSGDRTQRFGFGCPIDWWFWVALLSQASGSLE
                     GLESGRSLGGKAGLEAIQLWQHLVTGLRVMKPPPGRDYNAWEQTNQDFLAGRVAMIWT
                     STAFLKYLEQNARFPVIAAPLPAGERHAVPTGGTHWVITKASPVDDQVAAARFLEFMF
                     RAENVIEWATRTGYIPVTLDAIDRLTERGHYQAHPNEAVALEQLAHAVPWPWSTQLFR
                     VQREVIQPNLERAVLGSESAKQVFARAARGAG"
     gene            9080..10405
                     /locus_tag="VFQ61_00830"
     CDS             9080..10405
                     /locus_tag="VFQ61_00830"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9953010.1"
                     /translation="MRWNTAGRVLWLVACFPGCREATVTQTEPSARPSAATTALAAPP
                     GSAAGSARTDAGGTGPNAASCPAGMALIPGGKFWVGSSARERFADDERPKFLTELAPF
                     CMDVTEVLAGPYAECVQQGRCERPKPTRRSCTFGRAGREQHPINCVTFEQARAYCAAR
                     GARLPSEVEWEFAARGGPEYRRYPWGSAGPDGHTCWKHNGTCRSKEFEAGAFGLYDVS
                     GNVWEWTDTWYGPYPFPPERGFAKVYRGGSFSRRFEKWMHTRLRNRALPGDEGSHLGF
                     RCALNPVGVSCPDGKDAQGHCLHVVLDAECEAGQLWNGVRCARAGAPRCPAGLVEQAG
                     FGCVVPTLDEPAPGPSSEKRKTESADSSSSEARAEKRRQKEKEAATLAEVRRSRSPEF
                     DSDCRLNQPSRPHAYRYVAGTHAARTQAGKTAGCRNRDVGVGWNSACCP"
     gene            10605..11864
                     /gene="fabF"
                     /locus_tag="VFQ61_00835"
     CDS             10605..11864
                     /gene="fabF"
                     /locus_tag="VFQ61_00835"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET9953011.1"
                     /translation="MRRVVVTGLGVVSPLGVGAELSFQRLVEGRSGVRPVTHFDVGDL
                     PTKIAGQVPRGSNPGEYEPTRYVAAKELRRMDEFMVFALAAAEEATRDAGWQNPPDEE
                     RHRTGVLIGSGIGGLPSIAENAIKLHDGGPRRISPYFIPGSLVNEAAGVVSIQYGFSG
                     PNHAVVTACATGAHALGDAGRLIALGDADVMLAGGTEAATCRLTLAGFAIMRAVSTGF
                     NDRPEQASRPWDKQRDGFVIGEGAGMVVLEELEHAKKRGARIYAELLGYGLSGDAHHV
                     SAPEPSGDGARRAMKAALERARVNPSDVDYLNAHATSTPVGDPIEIAAVKSLFGEHAK
                     KLSLSATKSSTGHLLGAAGAVEAIFTILSMRGAVIPPTLNLHEPDDGNDLDLVPLVAK
                     RRQIRYALSNSFGFGGTNAALVFGPAP"
     gene            11941..>12309
                     /locus_tag="VFQ61_00840"
     CDS             11941..>12309
                     /locus_tag="VFQ61_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9953012.1"
                     /translation="MIDSVHFENFKSLKNTSVQFGQLTVLVGPNGCGKSSVLEAMSLA
                     AQVCAPPPEEYSEYQLFAITRLPNLFSGRRKPQRLLTQGSKNPIRLSLRSVAGDDLEL
                     SVPVRAPHDRSVSGEAKLTIL"
BASE COUNT         2237 a         3796 c         4148 g         2128 t
ORIGIN      
        1 ttcagatcga tgcagccgtg acgcgcaatc tgatcgacca tgtgagtcag gaacgggagc
       61 ggcgtcacga tgtcgctctg cccgctgccg tccaagttca cttcgacgga gatctgcgtc
      121 tctcgggtct ttctctcgac gactgcaact ctcttcatgc gcttgctcga atcagggtcc
      181 acggttcgat ccgaaaggct cggatctccg ccaggcaggc ccgtagttac ttcaggtctt
      241 caaccttcca ggcgccgtgt tcgcgaagca attccagaga gccgccggcg ccgtaggcga
      301 gtgtggcgcg atcgccgagg agctcgaagc gggcacttgg cagggcggct ttgagggcct
      361 gcatcatgcg ggcgacgtcc tccttctggt ccacggtcca agcctgcttg agtgaggcag
      421 gcgtgagctc gtgcttctgc gcgtccggca cgaagcgcag gagcacgtcg taacgctggt
      481 tttcgtaggc tcgcacgaaa gccgccaccg cgcgttccgg ggtcgcttgg ctatacagat
      541 cgacgctcga agcatcgacg cgccaagccc cgttctcgta gaccaggagc aagggcggcc
      601 cgttcgatgg cgtcaccgtg gccgtgacca acggcggcga agccgggcgt tgcaagctct
      661 tagcgaactc cgaaagctct tctgggtttt ccttgagaat gcgctcgaag ctctcgaaag
      721 gaatattctt ttgcgcctgc ttactgagca aggagtaggc ctcgcgggcc cggccgccct
      781 gcaccgctcg cgcgtacgcc tgaagcgttt ggtccggctg cgcggaggcg cccgcgctcg
      841 cacaccccac ctgcggccag gtgaggggaa cgagcgtgca ccacacgagg gcggacgcac
      901 ggacccgtcg aagggcctcg gaccacactc gtttcttcaa ccagctagcc gggcctggag
      961 gcgcgccatt cggcgcaacc agtacgtcgc accggcttcg gtcgcgcgga agcgcgcttg
     1021 gccaagaccc agggctgacg agggccatgg gggaagaacg agccaacatg gtgcgggtcg
     1081 cacttaccac agcgcttccg gtgttgcgac ttcggcgcgc gcgaccgttg cggctttcaa
     1141 tcgggtggct gctttttcgt ctggatctgg acgtctacgg gggtcgtact aatctgggcg
     1201 catgtgccca gttgccggga cgagacccga gcgcatcgcc atactgccgc tccgaaattc
     1261 ggttctattc ccgatgtccg tcgtgcccat caacgtgggg cgccaacgaa gtgttcggtt
     1321 ggtcgaggag ctgctcggca aagacagcgc gctcgtcggc gtgctgacgc aacggaacgc
     1381 cgatacggtc gagcccacgt tcgaagactt gtacggcatc ggtaccttgg cccgggtcgt
     1441 caaggtcatt cgcctcggcc cctcgaacta cagcgtggtg ttgaacggcc tcggtcgttt
     1501 caagctgctc gaaccgcaag ggctcgagcc ctacatgcgg gcggatatcg agcgcgtgcc
     1561 agagctcggg caaaccaacg ccgagttgac gcgcttgggg cagaagctgc gcgagaacac
     1621 gcgggaggtg ctgggcttac tgccgaacct gccgaaagag accgcgagca tcctcgataa
     1681 cgtccgcgag cccggggctc tggccgactt gattgcctcg aattttcccg aggaacacgc
     1741 cagcatccac gtgcggcagc gcatcctgga agcgttcgac gtgaaggccc gggtcgagct
     1801 cgtgctcggc atggtgggcc gtcagctcga ggtgctcaag gtgcgcgacg aaatctcgtc
     1861 gcaggttcaa aatgaactca gccgctcaca gcgtgactac gtgttgcgcc agcagatgcg
     1921 ctcgattcgc gaggagctcg gagagagcgc cgaagacgac gagatcgagc agctgcgcga
     1981 acgcttctcg cgcgccgagc tacccgcaga ggcggagcgg gccgcacgca agcagctggc
     2041 tcggctgagc aacatgcagc cgcaatcggc ggaataccag gtcacccgca actacgtgga
     2101 gtggctcgcc gatttgcctt ggaatcgccg tacgcccgat cgcgtggacg tcaaagaggt
     2161 gcgccgttgc ctggacgagg atcactacgg gctcgagacc gtgaagaagc ggatcgtcga
     2221 gtactgcgcc atccggcagc tgcgtaagga taagcgcggg ccgatcctgt tgttcatcgg
     2281 tcctcccggc gtgggtaaga cgtcactggg gcgctcgatt gcgcgggcca tgggccgccg
     2341 ctatgggcgg atatccttgg gcggcgtgcg ggatgaggcc gagattcgcg gtcaccgccg
     2401 cacctatgtc ggcgcgctgc cgggtcgcat cattcaggcc ttgaagaagg ttggctgtcg
     2461 taaccccgtg ctcgtgttgg acgaggtgga caagatgggc gtcgatttgc gcggggatcc
     2521 cgccgcggcg ttactcgagg tgctcgatcc cgctcagaac gacagcttcg tcgatcacta
     2581 catcgatctg cccttcgatc tttcggaggt gatgttcatc gccacggcca attaccgcgg
     2641 caacatcccc gaggctctga aagatcgcat ggaggtgatc gatgtgctcg gctacacgcg
     2701 caccgagaag cgcgcgatcg ccgagcagtt cttggtcaag aagcagctca aggagaatgg
     2761 gctgaaagac gagcaattgt ccttcacgcg cgaaggcatc gagacgctcg tcgacttcta
     2821 tactcgggaa gcgggcgtgc ggagcttgga gcgagagatc gcggccgtgt gtcgcgacgc
     2881 gacggtgagc ttcgccgagg ggcgccgttc agggcacctg cgcgtgacgc ccgaagtggt
     2941 gcgcgaaatt ctcgggccgg agaagcatcg ccccgaacag accgagcgta agctcgctcc
     3001 aggtgtggcg attggtctgg gcgtggccag cggcggaggg gatctcctga tcgtcgaagc
     3061 cacgcgcatg ccgggcaaag gcgaggtggt gatcaccggc agcatgcgcg gagtgatgcg
     3121 cgagtcggcg acgacggcgg tttcgttcgt gcgctctcgg gccgaccgct tgcgtttgga
     3181 tccggagtgg ctgaggaaga tcgatctgca cttgcacgtg ccgcgcgccg cggctgtgcg
     3241 tgacgcggcc agcgccggtg tggccatgtt cgtggcggtg gtttcgttgt tgctgggcgt
     3301 gtcggcgcga tccgatgtcg cgatgacggg cgagttgacg ttgcgtggcg ccattttgcc
     3361 ggtgacgggc atcaaggagc aggtgctcgc ggcacatcgt tcgggcattc gccgggtgct
     3421 gatccctgcg cggaacgagc gtgactttgc ggacgtgccg gatgaggtcc gccaagacat
     3481 cgaggttcac ttcgtcaacc gtctggatga ggtgctcgcg ctcgtgctcg agcagccgag
     3541 cgcggacgag gccagcgcgc gagacgaatc ggccggtgac ggcgcgcaag gtgacgaggc
     3601 ctctccctaa aaagctctaa tcgaggggcc cgcgtggatg cgccgggctc cgaataggca
     3661 ggctctgaaa gatagggctc cgatccgcgg ggcccaacat atggctgtcg aggtcgggcc
     3721 accgaggccg agccgccaag gatgtatgag gatcaagagt tggcttccgc ccttcgagca
     3781 gcgtgccctc gtgctgtcca cgctgctcgc ggccgcactt gggttcgcgc cgcagcttgc
     3841 cgggcccggc tatgactcgg cgctgatcgc gggtgcgctc gggccgggct ggtgcggcgt
     3901 cgtcgcgagc tggtcggtgc tgcaatccga ggagcccagc gggcggacgg cctatggacg
     3961 aggggtgcgg atcggcttgt gctcgtgggc aaccgcgttc ctggtttgct tactgcacgg
     4021 ccttcgggtg ggaatgtgcg atcccgtcga ggggctttgg ctctggctgc tcgggcccgg
     4081 cttcgggagc gtgctcggcg gagtgagcgg cgccctcgcg ggcttgtgga ttcgtgcgca
     4141 cgccagctcg tggagtgtgc gttggagccg gtcgatcggc gcaggcttgg gtctggcggg
     4201 cccgttgctc gggatcagcg tgagcttgct gcgcttttac acgagcccga tggtcttcgc
     4261 ctacgatccg tacttcggct atttctcggg tccgctctac gacaccgtga tcggctcgtt
     4321 gtggccgttg gtcacgtatc gcgccggaac cctggccagc ttgttggtgc tcgcattggc
     4381 tacgtcactg gtgacttggc cggcgaacgg agcgccgcgc tggaacaaac ccagcggact
     4441 acaagtgctc ggcctcgtgc tgttcacgct gctgagcgtt gggatcagcg cggcgggcac
     4501 gcgactggga catgccagca ccgcgcgcag cattcagcag gagctcggcc gggagagtca
     4561 gttcgggcgc tgtagaatcg tgcactcgcg cggcgtaact gaacgggatg cccagcgtct
     4621 cggccgagag tgccacgcgc acctgcgcca aatcgagcgt tttttcggcg cggcggggcc
     4681 agccaccgtg acggtctacc tgttcgcgaa tgaccaggaa aaaggccggt tgatgggtgc
     4741 ggcacacacc tatatcgcga agccgtggcg ccacgaagtc tacgtgcagg ccgcgggttt
     4801 cccgcatccg gtgctcggac acgaactcgc gcacgtcgtg tccggcagtt tcggtcaggg
     4861 gccgttcgag gttgccggga agctcggcgg attatggccc gatccggggc gcatcgaagg
     4921 agtcgcgacc gcggcttcgc cggacgagaa cgatgcgttg acgctcgagg aatgggcggc
     4981 tgccatgcaa cggctcgagc tgctcccacc tctcggctcg ctgttccagt tcgggttttt
     5041 cggtcgtaac gcggcgcaag cctacacggt gagcggggca ttcgtacgtt ttctgagggc
     5101 gagctacggc gcgtcggcgg tgcgtcgttg gtacggtgga gaggggctgc cgagtatcac
     5161 cggcaagagc ctcgcggatt tggagcagcg ctggcgcgcg gagttgcgcg agctcacgct
     5221 gagtgatgcc gcgctggccg cggcgcgggc tcgttttcaa aggccgtcct tcttcgatcg
     5281 cgcttgccct cgcgtgatcg atcgcgaatt gggtgaggcg caggggcagc tggtgttggc
     5341 tgacttcgga ggtgcgcgcc gtgggttcga gggcgtgctg cggctagatc cgcacgactc
     5401 cgcggcgcgc ttggggctcg ccgcgtgcag cgcgcgaagc gggcaaatcg aggctgccgc
     5461 acgcagttat cgagcgctgc agagagcgac cgatgctgca ccttgggcgc ggctacaggc
     5521 tctggaagcc gaggcggatc tggccttgcg cagaggaaat ctggacgagg cacgcgcgct
     5581 ctatgcggag ctcgcacagc gcatcgtgga cgaggatcga ctgcgcagct tggacgtcaa
     5641 gcgcctggcc gagccggggc tcgcgcagcg agcgatcgtg gccctgctga tcggggacga
     5701 gcttgggccc gcttgggatt ccgcggcccc gctactcggg gaatggtcgg catcgaatgc
     5761 cagcgatggc ctcgcggatt acctgcttgc caagaatcta ttcggcagga atcgcgctcg
     5821 ggacgcgcaa cggtacatcg aacgcgctct gacgcgcgcg ttgcccgagc cgcgcgtgca
     5881 cgacgaagcg ctgcgcttac agctactggt gggctgcgcc tcgaacgacc cggagcgtgc
     5941 gaggcctgca tacacgagct tgctggctcg accgatcacg cgcgccaagc gcgttgcgtt
     6001 ggagcgcatc gcggagcgct gcggaatcga aagcgctcca aatcccgttg cgccgtaggg
     6061 agcagtggga gcctctcaac tgctgctgcc cagtacggcc cgcagcaaat ggcgttgggc
     6121 gacggcaaac agggccaaca cgggcgcgct caggatcacg ttgccggcca ttacgatgtg
     6181 ccagcgcacg cctgtgccct gctcacgcaa caacgctacg ccaaccggta gcgtgcgggc
     6241 tcgatcatcc gtggtcatca ctagcggcca gaagtagtcg ttccaatgat aaacgaagct
     6301 aaatagaaac acggccacca gcgtcggcac gatcagcgga ggtagaagcc cgtacacgat
     6361 gcgaagctcg ctcgagccgt cgatgcgtgc agcctcgatg accgcgtccg gaaccgacaa
     6421 caaggcttgg cgcaccaaga acgttcccaa cgcgctgacc ccaaagggca ggatgagtga
     6481 tgcgagcgtg ttgctcaacc cgagcttggc gaaaagcatg aacgtcggaa cgaagcgcag
     6541 ctgagcgggc aacaggagcg tcgccaggat caacaccaag cacaatgagc gcccccgaaa
     6601 gcgcaactta gccagggcgt agcccgcggg aatcgccaaa agcagctgca aaaccgcgat
     6661 gccaatcgcc atcgccgccg tattgagtaa atagcgtgcg atcggcatgc tcgcgagcgc
     6721 acgctcgtac gcctggccat tccacgaccg cggtagccac gcgtgcggag cctgcacgat
     6781 ctccggcaat gtctttaggc tggtcaacac catccaggcg tagggaaaca gccaaatcag
     6841 cgccacgggc aatagccagg cgagctgcca cgccgtgccg cttcgcgaac gggccacacg
     6901 ggcgaggctc acagcgcctc tcgacggcga gagatccgca gctgcagcgc cgtcagtacc
     6961 agcaaaatta cgaagaacag catcacctcc gcgctcgcgc ggccaacgcg caggtccatg
     7021 aacatctgct cgtaaatcga atacacgaag accgtggtcg aacggaccgg gccgccttgg
     7081 gtcatgactc gcaccacgtc gaaggcttga aaggacaaga tgaagctcgt gatggccacg
     7141 aacgccgtcg ttggcccgag cagtggcagc accacgtggg ccagccgcgc cacgcgccct
     7201 gctccgtcta gctccgcggc ttcgagcacg ctttgcggaa tgctgccgag cccactttgg
     7261 aacaccacca tggcgtaacc agccgttttc caaacgagta cggccgacaa cgccaacggt
     7321 gcggtggtga cgttggcgag caggcctttg gaacccggcg cgaggcgctg aatcaacgcc
     7381 gagaccaaac ccgcatctgc atccagcagc cacaaccaga gcaggccaac gctcacccag
     7441 gacaccacgt acgcgctgaa cacgctcgag cgtatgaagg ccacgagttt ccctggccgg
     7501 ttcaaagcca aagccaagct caaccccgca agcatcgaac cggccacact gagtacactg
     7561 atcagcaacg tcgtgccaag cacggtcgcg aaagacccgc tgctgagcag cgctcgatag
     7621 ttcgagaatc ccacgtagcg tgcaggcgtc agtagatccc aatcgagcag gctgtagtag
     7681 gccgcgagca gcaatgggta gaagtagaac agggccagga acgccagggt gggaccgagc
     7741 agccaagccg gatcgaagcg atgtgcaggt cgcatctcag ccggcgcctc gggccgcgcg
     7801 cgcgaacacc tgtttggccg attcagagcc caaaactgct cgctccaagt tcggctggat
     7861 cacctcgcgc tgtacgcgga acaactgggt cgaccagggc catggcacgg cgtgcgcgag
     7921 ctgctcgagc gccaccgctt cattgggatg agcttggtag tggcctcgtt cggtcagtcg
     7981 atcgatcgcg tcgagagtca ccgggatgta tccggtgcgc gtggcccact cgatcacgtt
     8041 ctcggcgcgg aacatgaact ccaagaaacg tgcggccgca acttggtcgt ccaccggcga
     8101 agccttggtg atgacccagt gagtgccacc ggtgggcacg gcgtgccgct cacccgcggg
     8161 gagaggagcg gcaatcaccg ggaagcgggc attctgttcc aagtacttca ggaaagcggt
     8221 ggaggtccaa atcatcgcca cgcgaccagc caggaagtct tgattggtct gctcccaagc
     8281 gttgtagtct cgcccgggtg gcggcttcat gacgcgaagt ccggtgacca gatgctgcca
     8341 aagttggatc gcttcgagcc ccgcctttcc gcccaagctt cggcccgatt ccagcccttc
     8401 cagagatccg gaagcctggg agagcaaggc cacccagaac caccaatcga tcggacaccc
     8461 aaacccgaag cgctgcgttc gatcgccgct ccgtagcgtg agcgcgcgtg ccgtctcgac
     8521 gagctcgggc caggtgcgcg gcgcgtggag cccagcttcg gcgaacacct gaccattcaa
     8581 gtacgcgatt ggggtagatc gattgaaggg taaggcccag agcggcctct ccgcgccgcc
     8641 gatgaacgag ccagcctgtc cgagtgaagg gtgcagcctg agcgttgcca aagaagtaaa
     8701 ggcatccagt cgctgcagcg cgcctgcttc ggcaaggtag gggatcactt cacccacgac
     8761 gtggctcaac gcggggggat cccccacggc gagcgcgact cgcagcttgg cgagtccctc
     8821 gaagtagtcg ccttgaaaga cgccctcgac gaagatctcg ccctgcgaag cgttgaactt
     8881 cgccacgagc gcctccaata cctcgcggtt cttgcccccg taagtgaacc agagcgcgat
     8941 tcgatgtcga ccggcccgca acttcgagtt cgattcacac gcgagcccca atgtaccggt
     9001 tacgaacgcc cggcggccga cgcggaacgg gtgtcgaatg gtctggggca tgcgcagcac
     9061 ggcatgctag acgagttgag tgcgctggaa caccgccggg cgcgtgctgt ggctcgtggc
     9121 ctgtttcccc ggttgccgag aggcgactgt tacgcagacc gagccgtccg cccgccccag
     9181 cgcggcgacg accgctttag ccgcaccccc gggcagcgcg gcaggttcgg cccgtactga
     9241 cgcgggcggc accgggccta acgccgcgag ttgccctgcg ggcatggcgc tgattcccgg
     9301 cggtaagttt tgggtcggct cgagtgcgcg cgaacgtttc gcagacgacg agcgtccgaa
     9361 gtttctgacc gagcttgctc cattttgcat ggacgtgacc gaggtgttgg cgggacccta
     9421 cgccgagtgc gttcagcagg ggcgctgcga gcgaccgaag cctacgcggc gatcgtgtac
     9481 ctttggccgt gcgggacgcg aacagcaccc gatcaattgc gtgacgttcg agcaagcgcg
     9541 ggcgtactgc gcggctcggg gcgcccggct cccgagtgaa gtcgaatggg agttcgccgc
     9601 ccgcggtggc cccgagtacc gtcgctaccc ttggggttcg gcggggccgg acggacatac
     9661 ctgttggaag cacaacggga cttgccggag caaagagttc gaagccgggg ctttcggtct
     9721 ctacgatgtg agcggcaacg tctgggagtg gaccgacacc tggtacggac cttacccatt
     9781 tccgcccgag cgcggcttcg cgaaagtcta ccgcggaggt agcttcagtc ggcgcttcga
     9841 gaaatggatg cacacgcgct tgagaaatcg ggccctgccc ggggatgaag gctcacacct
     9901 agggtttcgc tgtgcattga acccagttgg ggtgagttgc ccggatggca aggacgctca
     9961 agggcactgc ttgcatgtgg tcctcgatgc cgaatgcgaa gcgggtcagc tctggaatgg
    10021 cgtacggtgt gcgcgtgctg gcgcgccccg ttgcccagcg gggttggtcg agcaggctgg
    10081 cttcggctgt gtcgtcccaa cactggatga accagcaccg ggtccgagct ccgagaagcg
    10141 gaagaccgag agtgccgatt cgagttcgag tgaggcgcgg gccgagaaac gccggcagaa
    10201 agagaaagaa gcggcgactc tggccgaagt gcggcggagt cgcagccccg agttcgactc
    10261 ggattgccgc ttgaaccagc ctagtcgtcc ccacgcctac cgttatgtcg cgggcacgca
    10321 cgcggcgcgc acacaggccg gcaagactgc cggctgccgc aaccgcgatg ttggagttgg
    10381 ctggaattct gcgtgttgcc cgtgaagcgc ggaacgcgcg acgtcatgca cctcgaaccg
    10441 tacttacgaa cgtggcgctg gagtacgcac gcattcgtcg gctcgggcaa cttgcaacga
    10501 gagtgtgacc gcgctacgcg cacagccagc atgcatttcg agagccgtaa ccagaagagt
    10561 aggctggatt cgcgcgatcc ttcccaggcg cgggcggtgc gaccgtgagg cgcgtcgtcg
    10621 ttacagggct gggtgtcgta tccccgttgg gcgtcggcgc agagttgagc tttcaacgct
    10681 tggtcgaagg gcgttcgggc gtgcgtcccg tcacccactt cgatgtcggc gatttaccaa
    10741 cgaagattgc gggtcaggtg ccgcgaggca gcaaccctgg tgaatacgag cccacgcgct
    10801 atgtcgccgc caaagagctc cgccgcatgg acgagttcat ggtgttcgcc ctggccgcgg
    10861 cggaggaggc cacgcgcgac gctggttggc agaacccgcc cgatgaagag cgccaccgaa
    10921 cgggcgtatt gatcggctcc ggcattggcg ggctcccgag catcgcggag aatgccatca
    10981 agttgcatga cgggggcccc cggcgtatct cgccgtactt catcccgggc agcctcgtga
    11041 acgaggctgc cggtgtggtc tccattcaat atgggttttc agggcccaat catgcggtgg
    11101 tgacggcgtg cgcgaccggg gctcacgcgc tcggggacgc gggccgtttg attgcgctcg
    11161 gggatgcgga cgtgatgttg gcgggcggca ctgaagccgc aacttgccgg ctcacgttag
    11221 cgggctttgc gatcatgcgc gccgtttcca cgggcttcaa tgatcgaccc gaacaagcct
    11281 cgcgcccctg ggacaagcaa cgcgatggct tcgtgatcgg cgagggcgcc ggcatggtgg
    11341 tcctcgaaga gctggagcac gccaagaagc gcggagcgcg aatctacgcc gagctcctcg
    11401 gctatggtct atcgggtgac gcgcatcacg tgagcgctcc cgaaccgagc ggtgacggcg
    11461 cacgccgcgc catgaaagcg gctctagagc gcgcccgcgt gaaccccagc gatgtggact
    11521 atctgaacgc ccacgccacg tccacgcccg tgggcgatcc gatcgaaatt gcggccgtca
    11581 aatcgctatt tggcgagcac gcgaagaagc tcagcctgtc cgcgaccaag tcatcgacgg
    11641 ggcacctact cggcgcggcc ggagcggtcg aggcaatctt cacaatcctc tcgatgcgcg
    11701 gcgccgtgat cccgccgaca ttgaacctcc acgagcctga cgacggcaac gatctcgacc
    11761 tcgtgcccct cgtcgccaaa cggcgccaga ttcgctacgc gctctcgaac tccttcggct
    11821 tcggcggcac caacgccgcg ctcgtcttcg gccccgcgcc gtgagggaaa agcgcgggtt
    11881 tgtagcttca agcaaactgc agtattgggt gggtgcccgc taggacgaga aacggcccac
    11941 atgatcgaca gcgtccattt cgagaacttc aagagcttga agaatacgag cgtgcagttc
    12001 ggccaactca cggttctagt tgggccgaac ggatgcggaa agagcagcgt tctcgaggcg
    12061 atgtccttgg ccgcgcaggt ctgtgcccca cctcccgaag agtactcgga gtaccagctt
    12121 tttgcgatca cgcggcttcc gaacctgttc tcgggccgtc ggaagccgca gaggctgctg
    12181 acccagggct cgaagaatcc cattcgactc tcgctgcgca gcgtggcggg ggatgatctc
    12241 gagttgagcg ttcctgttcg agctcctcac gaccgcagcg tgagcggtga agccaaattg
    12301 acgattctt
//
LOCUS       DASTKU010000027         5915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_673406, whole genome shotgun sequence.
ACCESSION   DASTKU010000027 DASTKU010000000
VERSION     DASTKU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673406"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VFQ61_00845"
     CDS             <1..772
                     /locus_tag="VFQ61_00845"
                     /inference="COORDINATES: protein motif:HMM:NF028498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HET9953013.1"
                     /translation="QTTSYSDAQLATWITTLGPAMKPLGIKVMGPETMNACGFSKYFT
                     AIKNTAAAWDAVDIFASHEYGCGTLPTQTSISAANKEYWETEVDTGKASGDASGDGIA
                     SALLMATTMHNDLTKPNLNAWHLWWVYNASGNGGCLYDTTTNVWTKRLWVMGNYSRFV
                     RPGSKRVGTSGTPPSGVFVSAYINPTNSSLSIVAINTNTSSKDVSLYISGGAPCTLTP
                     YETSANKDLGQGSVLTVSQSRVTVTLAAQSVTTLTGTP"
     gene            complement(1024..1212)
                     /locus_tag="VFQ61_00850"
     CDS             complement(1024..1212)
                     /locus_tag="VFQ61_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953014.1"
                     /translation="MPNEVYEKAPLSRGADIAVGVGLAALFAGSAVYGFKGTSACRRA
                     QPKSGSGPQPASTLPALH"
     gene            1372..2934
                     /locus_tag="VFQ61_00855"
     CDS             1372..2934
                     /locus_tag="VFQ61_00855"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9953015.1"
                     /translation="MGQMVLFIVKEIEGAEPLGALYVAGCLAHAGHECRFVGTRGNRV
                     LDDVRRFEPDVIAFGATTGLHRYYLGLNAHLKAHYPNCVSLMGGPHPTYYPEVIATPG
                     LDVICQGEGEDAAVELCNALTNGEDYRFIPDLWVKREGVVYKNPARELRRDLDSIPYP
                     PRELLYAYDDALRRRPLKSFTTNRGCPFPCSYCFNASLVDHYGSSWKKVRIRSPENVV
                     GELVQVQRSGPLQIVAFRESIFVYSARWLREFGELYRREVKLPYYCHVRADLMTDEMV
                     ELLAWSGCHTVNLGIETANPRLANEVLRRQIDMQKLAAGVRRLKRAGIVVFADNMLGI
                     PGGGLEDDLATLKLNIDLDVDYAAATLCTPYPGTGIARYAEEHGYFDGDFDLIDDSYY
                     TESVLRFGSAAEKLQIENLHKLFAITAAIPALLPLTRRLLRLPPNDYFYAAFRAWYLI
                     CHMTEVMPRRPDWEQVKESLLSIFGVYRGVDENRFAAPEPESLRFVDNPGFEVKASRV
                     KLRMQREQAAHA"
     gene            2927..3319
                     /locus_tag="VFQ61_00860"
     CDS             2927..3319
                     /locus_tag="VFQ61_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953016.1"
                     /translation="MPDEREDVERERARRVVIRRYKRRLKAAAALVLSVAAGTFLACQ
                     RESDKPGARPAPAPNPTTPEISNLPAAESGGGANETNSDRSSGGVASGTVESGGGPSS
                     GAGGAGRRNQVDRHEHRKGMPVRDNLVE"
     gene            3430..4323
                     /locus_tag="VFQ61_00865"
     CDS             3430..4323
                     /locus_tag="VFQ61_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953017.1"
                     /translation="MKAPCAFCFTAMMLVCCSSSKNSSSPGSGGGVNASGGANVGGAS
                     GGRDAAYGGAISGAPASGGFASGAGGADGGAGGEGGSNDCPALIDSDGNIEAACVGVH
                     GHWYSLADSLGPNGAAPGMCQSVGMHPDNECSQPEPLQFVTIGEGAAIEVCTQGTLAK
                     VGLLDGEPDFINQWGASIGFDFAATPEARMTFDAAGAGVRGVAFELNQRPLPGLRVEL
                     YDARTAAGHDAAYWGATSSYPSSPIKVGVNEITWDQVASPVASVPELDTTQLVSMVFH
                     VPASTVKSTYQFCISNVRLLR"
     gene            complement(4375..5523)
                     /locus_tag="VFQ61_00870"
     CDS             complement(4375..5523)
                     /locus_tag="VFQ61_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9953018.1"
                     /translation="MRETQPRAWLRVGFLLFAAGWGANHFTSLLLVYRARLNLDAAAP
                     GLLFAMYALGLAPGLLLAGPLSDRHGRRALVLPGACVALGASALLGASGTWFGALLLG
                     RFLYGFGAGSVMSAGAVWLMEISASSAPGTGARRATIALSSGFGLGPFVTGALAQFAP
                     APTKLPFALHVLVLAGALLGVRRVRETSRGTGKRSSPLLRVQLMSGNWPRFLRRIAPM
                     APFVFGFPALAFAALPNVLGTGALGPLPFVYTGLLCGLTLAAGVIAQPLTLRFTPQTS
                     ARIGLVTGAVGVALGALVVATHASWALLVVGPVLGVAYGVCMTAGLQASQRLASPEGR
                     GGVTGLYYVLTYVGFAAPYLLALAMRAVTPELALEVTAAVALVIALFV"
BASE COUNT         1179 a         1974 c         1827 g          935 t
ORIGIN      
        1 ccagactacc tcgtactccg acgctcagtt ggcgacctgg attactaccc tgggccccgc
       61 gatgaagccc ctcggcatca aggtcatggg accagaaacc atgaacgcct gcggattttc
      121 caagtacttc accgcgatca agaacaccgc cgcggcctgg gatgcggtcg acatcttcgc
      181 gagccacgaa tatgggtgtg gcacgctccc cacccaaaca tccatctcgg ccgcaaacaa
      241 agaatactgg gagaccgagg tagatacagg aaaagcgagc ggagacgcct cgggcgacgg
      301 cattgcgagc gcgctcctga tggccacgac catgcacaat gacctcacca agccgaacct
      361 caacgcttgg cacctttggt gggtctacaa cgcgagtggc aatggcggat gcctctacga
      421 tacgacgacc aacgtctgga ccaagcgcct ttgggtcatg ggcaactact cgcgcttcgt
      481 tcgtcccggc tctaagcgcg tcggcacttc cggaaccccg ccttcaggcg tcttcgtgag
      541 cgcatacatc aacccgacca atagctccct ctcgatcgtg gcgatcaaca ccaacacctc
      601 atcgaaggac gtctccctct acatctccgg cggcgccccc tgcacgctca ccccctacga
      661 gacatccgcc aacaaagacc tcggccaagg ctccgtcctc accgtttcgc agtcgcgcgt
      721 caccgttacc ctcgccgccc aaagcgtaac gacgctgacc ggaacaccgt aacgcgcggt
      781 cgcggcgtcc gcacgaagcg tacgctcgta ccgcaacctg aaccaccgca ggcgatcgta
      841 cccgcaacaa ccttagaccc actcctaaat cctggaccga gcctggccaa gagacgcgag
      901 cagtccaagg cgagacgacg aggggtactg aaggatcctg agactgaagt acgccgagga
      961 gaagcaacga acgaatgcga agcggatctg ccgcgtagcg acgggaggga tttcgatcgg
     1021 ttcttagtgc agcgccggca atgttgacgc tggttggggc cccgaaccgc tcttgggttg
     1081 tgcacgcctg cacgcgctcg tccctttgaa tccatacacg gccgagcccg caaacagcgc
     1141 ggccaggcct actccgaccg cgatgtccgc cccacggctc agcggagcct tctcgtaaac
     1201 ctcgttcggc actcccatac tcccaaagtg ctattgcgcg aacgggggtg gccgcgcacc
     1261 gcacgtccga gcagtgctcg ttcgcatgcg tcaaagattc gtaaaatcag cccaccgaac
     1321 gcgctcgctc ccgggagaga gaatatagtg cccgctgctc aggagagggc catggggcaa
     1381 atggttctgt tcatcgtcaa agagatcgag ggcgccgagc cgctcggcgc gctctacgta
     1441 gcgggctgct tggctcacgc gggtcacgag tgccgcttcg tcgggacgcg cggcaaccgc
     1501 gtgctcgacg acgtgcgccg cttcgaaccc gatgtcatcg cgttcggcgc aacgacggga
     1561 cttcaccgct attacttggg tctgaacgcg cacctcaagg cccattatcc gaactgtgtg
     1621 agcctcatgg gcgggccgca ccccacgtat tacccggagg tgatagcgac gcctggactc
     1681 gacgtcatct gccagggcga gggggaagac gccgccgtag aactctgcaa cgccctgacg
     1741 aacggggaag actaccggtt catcccggac ctgtgggtga aacgagaagg ggtcgtctac
     1801 aagaacccgg cgcgcgagct gcggcgcgat ctagactcga ttccgtaccc gccgcgcgaa
     1861 ctcctgtacg cttacgacga cgcgctgcgg cgccggccat tgaagtcttt cacgacgaac
     1921 cgggggtgcc cgtttccgtg cagctattgc ttcaacgcat cgctcgtgga ccactacggc
     1981 tcgagctgga agaaagtgcg catccgcagt cccgagaacg tggtcggcga gctcgtccaa
     2041 gtgcagcgtt cgggcccgct gcaaatcgtc gcgtttcgcg agagcatctt cgtgtacagc
     2101 gcccgttggc tacgcgagtt tggagagctc taccggcgag aagtgaagct cccttactac
     2161 tgccatgtac gagccgattt gatgaccgac gagatggtcg agctcctcgc ttggtcgggg
     2221 tgtcacaccg tcaacctggg catcgagacc gcgaatccgc gcctcgcaaa cgaggtgctg
     2281 cgccggcaaa tcgacatgca gaagctcgcc gcgggtgtac ggcgcttgaa gcgcgccggc
     2341 atcgtcgtgt tcgccgacaa catgctcggg attcccggcg gcgggctcga agacgacctc
     2401 gcgaccttga agctcaacat cgatctcgac gtcgattacg cggccgcgac gctgtgcact
     2461 ccgtatccgg ggacgggcat cgcgcgctac gcagaggagc acggctactt cgacggcgat
     2521 ttcgatctca tcgacgacag ctactacacc gagtcggtgc tgcgcttcgg ttccgccgcc
     2581 gaaaaattgc agatcgagaa cctgcacaaa ttgttcgcga tcacggcggc gatccccgca
     2641 ctcctgccgc tcaccaggcg gctgctgcgc ttgcctccga acgactattt ctacgcggcg
     2701 tttcgcgcgt ggtacctcat ctgtcacatg accgaagtga tgccgcgccg acccgactgg
     2761 gaacaagtga aagagagctt gctcagcatc ttcggcgtgt accgcggcgt ggacgaaaat
     2821 cgattcgcgg cgcccgaacc cgagtccttg cggttcgtcg acaaccctgg tttcgaggtg
     2881 aaagcgtcgc gggtgaagct gcgcatgcaa cgggagcaag ctgcgcatgc ctgatgaacg
     2941 cgaagacgtg gagcgtgagc gcgcccgtcg cgtcgtcatt cgtcgctaca agcgacggct
     3001 gaaggcggcc gcggcgctcg tgctttcggt ggcggccggc acctttctcg cctgccagcg
     3061 cgagtcggac aaacccgggg cgcgacccgc tccggcgccc aacccgacga ctccagaaat
     3121 ctcgaacttg cctgccgcag aaagtggtgg aggtgcgaac gaaacgaact ccgaccgcag
     3181 ctctggaggt gtcgcgagcg gcaccgttga aagcggcggc ggcccctcga gcggtgcggg
     3241 tggagcgggg aggcgcaacc aggtcgatcg tcacgaacac cgcaagggaa tgccggtgcg
     3301 cgacaacctc gtggaatgac ggggcaacgg ggccgaaaga aacctcgggt ggaccaggcc
     3361 ttcagtcccc acgcccacgg tggcatcgat gatgacccct cgttcccaat tttagtaagg
     3421 tgcgccgcca tgaaggctcc ctgcgcattt tgcttcaccg ccatgatgtt ggtctgttgt
     3481 tcgagcagca aaaacagctc ctcgccgggc agcgggggcg gcgtgaacgc aagcggcggc
     3541 gcgaacgttg gaggcgcgag cggcggtcga gacgctgcct atggtggtgc gatttcgggc
     3601 gcgccggcga gcggcggctt tgcgagcgga gcaggtggag ctgacggcgg tgcgggcggc
     3661 gaaggtggca gcaatgactg cccggctctc atcgattcgg atggaaatat cgaagccgcg
     3721 tgcgtgggag tacacggcca ttggtattcg ctggcggaca gcctcggacc gaacggagcc
     3781 gctcccggga tgtgccagag cgtcggcatg cacccggaca atgagtgctc gcaaccggag
     3841 ccattacagt tcgtgacgat aggcgaaggc gccgccatcg aggtgtgcac gcagggaacc
     3901 ctcgccaagg tcggtcttct cgacggcgag cccgacttca tcaaccagtg gggagcctcg
     3961 atcgggttcg acttcgctgc cacgcccgaa gctcgcatga ccttcgacgc ggcgggcgca
     4021 ggcgtgcgcg gcgtggcgtt cgagctcaat cagcgcccct tgccgggcct gcgcgtcgaa
     4081 ctttatgacg cacgcactgc tgctgggcac gatgccgcgt attggggcgc aacgtcctcg
     4141 tacccatcct ctccgatcaa agtgggcgtg aacgaaatca cttgggacca agtggcttcg
     4201 ccagtcgcgt cggttcctga gctggacacc acgcagctcg tgagcatggt gtttcacgtt
     4261 ccagcttcca cggtgaagag cacctatcag ttctgcatca gcaacgtcag gctgctccgc
     4321 tgaagctcga gccagcgagc tcccgtacga agcgttgctg cgtggcgacc tcactcaaac
     4381 gaagagcgcg atgaccagcg cgacggccgc cgtgacttcg agagcgagct caggagtaac
     4441 ggcgcgcatc gcgagcgcca gcaggtacgg cgccgcgaac ccgacgtagg tgagcacgta
     4501 ataaaggccc gtcacaccgc cgcgtccctc gggcgacgcc agccgttgag acgcttgcag
     4561 gcccgcggtc atgcacacgc cgtaggccac gccgagcacg ggccccacga ccagcaacgc
     4621 ccacgaggca tgcgtcgcga cgaccaatgc gcccaacgcg actcccactg cgcccgtgac
     4681 caacccgatt cgcgccgagg tctgcggtgt aaagcgcagc gtgaggggct gagcaatgac
     4741 cccggccgcg agtgtcagtc cgcacaagag ccccgtgtac acgaaaggca gcggccccaa
     4801 tgcgcccgtc ccgaggacgt tgggcaacgc cgcgaacgcg agagcgggaa agccgaagac
     4861 gaacggggcc atgggagcga tgcgacggag aaagcgcggc cagtttccag acatcaactg
     4921 aacacgcagc aatgggctgc tccgcttccc cgtgccgcgt gaagtttccc gaactcgccg
     4981 aacgccgagc agcgcgcccg ccagcacaag cacgtgcaat gcgaaaggca acttcgtcgg
     5041 cgcgggcgcg aactgagcga gagcaccggt cacgaacggg cccaagccga agccagagga
     5101 cagcgcgatt gtggctcgcc gcgcgccggt gcccggtgct gagctcgccg aaatctccat
     5161 cagccacacg gcgcccgcgc tcatgaccga ccccgcgcca aagccgtaga gaaaccgccc
     5221 caagagcagg gctccgaacc aagttccgct cgcgccgagc agcgcgctcg cgccgagcgc
     5281 gacgcaagct ccgggcagga cgagggcacg ccgcccgtgc cgatccgaga gcggacccgc
     5341 gagcaataac cccggtgcga ggccgagcgc gtacatcgcg aacagcaaac ccggcgcggc
     5401 ggcatccaga ttcaagcgcg cccgatacac gagtaggaga gaagtaaagt gattggcgcc
     5461 ccagcctgca gcgaacagca agaagccaac tcggagccac gcgcgcggtt gggtttccct
     5521 catttcggaa ttgcaggagg atgcgctgaa ccaggtgctg cgcgccacat agatataagg
     5581 ccgggccgcc cgtggcgaaa gtgcctcgag ccgcgccctc gccctcgccc tcgccctcgc
     5641 tgtcgccccc gtccgcgccc gcgcatgccc attgccgcaa ctcagactcg aacccgagcg
     5701 cgcgtgccca ttgccgcaac tcagcctcga acccgagcgc gcatgcccat tggcgcaact
     5761 cagactcgaa cccgagcgct ccttgcagaa ccgttcctct catcattggc ggccgccggt
     5821 tgaccaccag tcactgtcac accttgctat gccttggttt cgccttcgca cttccgcgca
     5881 acttgactgc atcgaccgct cccgccacag tctcc
//
LOCUS       DASTKU010000028         3643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_227436, whole genome shotgun sequence.
ACCESSION   DASTKU010000028 DASTKU010000000
VERSION     DASTKU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227436"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VFQ61_00875"
     CDS             complement(<1..1257)
                     /locus_tag="VFQ61_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HET9953019.1"
                     /translation="MRFHIERWGWIVVLAAGCADVGSQQEGTASKGSALTQQGPAVTA
                     NAPAAAAAPAAGTTRVYRSHANPADRQHPTGLLEMTPDQRKALQAATRKVTEVRPTPL
                     ALDRIKQHVATQKTNGQAARSASADVSALSGRVADPGTDLVTSAPADKSSVTLAAAAT
                     LPRSVDNSTLPAFPEIRDQGGIGSCVGFAVGYYLYTHELGLIAGWNNKNTSNTTKVSP
                     KWAYNLSNNGENNGTWASSVHGVLAESGALMWSDFPYNGDVLNPVNFREWPRTSTLWK
                     RALGYKSLGYSYLNVPTDAESLAQIKSVLLNGHVVNFATHIYSWQFDVIDNDPSTSAD
                     DAVVGQHIATWQNGGEGAHEATIVGYNDDIWVDLNANGRVDTGEKGAFKIANSWGSGW
                     ANNGYVWVAYDAAQFASQVPLGPASDS"
     gene            2130..>3643
                     /locus_tag="VFQ61_00880"
     CDS             2130..>3643
                     /locus_tag="VFQ61_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953020.1"
                     /translation="MSTDAVSSAKRESASAKSPIRVRGPRLGRRLWAWSTYGTKLLPH
                     WAWLVPLLAVIVGVYTAESSFGRPSRNWPIFTDDYDKLADAFRSGHLYLDRAVNPELL
                     QKDNPYDPKYENLWFGDSSLYKGRYYIYWGPLPALLQAIVKSALNIRYVLGDQYLVYA
                     FWAVGTCATVRALDRLTRRLFGPLPKPLFWVCALAFAFMNPVIHLLTNAGVYQAAISG
                     GQCFLLVGLGLALDVVHYARNRERQRVRMLAAGAAFAAAFGCRLSLAPISCLLLGITA
                     LYVGPLGQPKGSRRGTLVNAIRSAIWMGIPIVAGAALLALYNKLRFDDWFETGIKLQL
                     SFLPYRFSVTYFPANIYNYLLQHFSVSSTFPYVRDVANLRGSNLPWFIAVPPGYFTGE
                     CLTGMLRAVPLVWLAPVAVLMLLGVGTRRLWARDSGRARLYAHAVLCMLTMGTLSAIP
                     VFGLYVPSMRYLGDFTPGQALLGLMGGLGLYARVRTHPGKRRAVGALVVTLCVAT"
BASE COUNT          565 a         1169 c         1159 g          750 t
ORIGIN      
        1 cgaatcgctc gcggggccga gcggcacctg gctcgcgaat tgtgcggcgt cgtacgccac
       61 ccacacgtag ccgttgttcg cccagccgct accccaggag ttcgcgatct tgaaggcgcc
      121 tttttcgccg gtgtcgacgc gaccgttggc gttgaggtcg acccagatgt cgtcgttgta
      181 gccgacgatg gtggcctcgt gcgcgccttc tccgccgttc tgccaagtcg cgatgtgttg
      241 tccgacgacc gcgtcgtccg cgctcgtgct cgggtcgttg tcgatgacgt cgaactgcca
      301 agaatagatg tgcgtggcga agtttacgac gtggccgttc aagagcacgc tcttgatctg
      361 cgccagactt tcggcgtccg tgggtacgtt caagtagctg tagccgagcg acttgtagcc
      421 gagcgctcgc ttccacagtg tgctcgtacg cggccactcc ctgaagttga cggggttcaa
      481 gacgtcgccg ttgtagggga aatcagacca catcagcgcg ccgctctcgg cgagcacgcc
      541 gtgcacgctc gaggcccaag tgccgttgtt ctcgccgttg ttggagaggt tgtaagccca
      601 cttcggcgac accttcgtgg tgttcgaggt gttcttgttg ttccaaccag cgatcaaacc
      661 gagctcgtgc gtatacaggt agtagccaac ggcgaacccg acgcaggagc cgatgccacc
      721 ctggtcgcgg atctccggaa acgccggcag cgtgctgttg tcgacagagc gcggcagtgt
      781 cgccgcagcg gctagcgtca cgctgctctt gtcagcgggc gcgctcgtga ccaagtcggt
      841 gccggggtcg gcaacgcgac ccgagagcgc gctgacgtcc gcagacgccg agcgcgcggc
      901 ctggccgttc gtcttttggg ttgccacatg ctgcttgatg cggtcgagcg ccaatggcgt
      961 tggccggacc tccgttacct ttcgcgtggc cgcttggagc gccttacgct ggtccggcgt
     1021 catttcgagt aacccggtcg ggtgttgccg atctgccggg tttgcgtgcg atcgatatac
     1081 tcgcgtcgtc ccggccgcgg gtgcagctgc agctgccggc gcgttggccg ttaccgcggg
     1141 tccttgctgc gtcagcgccg agcccttcga tgccgtacct tcttgttggc tcccgacatc
     1201 ggcgcagccc gccgcgagca cgactatcca accccatcgc tctatgtgga atctcatgtg
     1261 cttcctttca cgaacccacc agacaatgcc gctcgagggg gattggttcg gtcccctgaa
     1321 cgtctcgagc gccccgcgag gcgctgacgg ttcgtcggct agttagagcg gcgagaagcc
     1381 gggaccttcg cccgtttctc cacgctttgc tcgtttcggc gaaacacgaa gtcggtcggc
     1441 tgctgggccc gcggtacgcg cagaccctgc gtgacgccta ccaccatcgc ccgtgatagc
     1501 ttccgatgat gggtgtcagc acgacatcgc tcggctggag cgtacgcggg cgctgctgct
     1561 cgcctcgatg caggctccgc caagcctgcc gcagctagcg cgactcgcga ggctgaacgg
     1621 ggtcaagttg aaggcggggt ttcgtgcgcg gttcgggaat tcggtatacg gctacctgcg
     1681 cacgcagcgg cctcgatgaa gcgcactgat tgctctcacg cggcggcctg aacgtgaccg
     1741 aagtggccgc gcgtgtgggt tacgacaacc caagcaagtt cgcggctgcc gttcgcaaaa
     1801 cagttcggca tcccgccgtc tgcgctccgc agctaaaagc ccgttgagcg cgtcgaacaa
     1861 tgcgagcacc gcgatgcggc tcattcgtcg gtgcgcaatg acgctagacg tagggtctgg
     1921 gacgccggcc gaatcctcga acacgacacg gggcgctact ccgaaacgta acggcaacgg
     1981 cgcggtggct caacggagga taaaatcctt gcaattcgct tcgggccggc caaactccgc
     2041 ttgaattgtt ggggccgttg tttgtctttg aagcatggtg atttgccgtc tgcgcgcccg
     2101 tgcgcgcatc aaatgggcgg agcggagctt tgagtaccga cgcagtgtcc tcggcgaagc
     2161 gtgagagcgc ctccgccaag agtccgattc gagtgcgggg gccacggctc ggacgccgac
     2221 tttgggcttg gtccacgtac ggcaccaagc tccttccgca ctgggcatgg ctcgttcccc
     2281 tgctcgcggt gatcgtcggt gtgtacacgg cggagtcatc gttcgggcgt ccctcccgaa
     2341 actggcccat tttcaccgac gactacgaca agctcgcaga cgcgttccga agcggacacc
     2401 tctacctcga tcgagcggtg aatccggaac tgctacagaa agacaacccg tacgatccca
     2461 agtacgaaaa cctgtggttc ggcgattcga gcctctacaa gggccgctac tacatctact
     2521 ggggaccgct gccagcgctc ttgcaagcga tcgtgaagag cgctctgaat attcggtatg
     2581 tcctcgggga ccagtatcta gtctatgcgt tttgggcggt gggcacgtgt gcgaccgtgc
     2641 gcgccttgga tcggctgacg cggcgcctgt tcggccctct ccccaagccg ctcttctggg
     2701 tgtgcgcgct cgcgtttgcg ttcatgaacc ccgtgatcca cctgctcacg aacgcaggcg
     2761 tctatcaggc ggcgatctcg ggcgggcagt gcttcttgct cgtcgggctc ggactcgcgc
     2821 tcgatgtggt gcactacgcg cgaaatagag agaggcagcg cgtccgcatg ctcgctgcgg
     2881 gcgccgcgtt cgcagccgcg ttcggctgcc gcctgagcct tgctcccatc tcgtgcctgc
     2941 tgctcggcat cacggccttg tacgttggtc cgctcggtca acccaaaggt agccgacgcg
     3001 gcactctcgt gaatgccatc cgaagcgcga tttggatggg gattccgatc gtcgccggcg
     3061 ccgcgctcct cgccctctac aacaagctgc gcttcgacga ttggttcgag accggcatca
     3121 aactgcagct gagctttttg ccgtatcggt tctcggtcac gtacttcccc gccaacattt
     3181 acaactacct gctgcaacac ttcagcgttt cgagcacctt cccttacgtg cgtgacgtcg
     3241 cgaacctgcg gggcagcaat ctcccctggt tcatcgcggt gccgcccggc tacttcacgg
     3301 gggaatgcct aacgggaatg ctacgcgctg ttccgctcgt gtggctcgcc ccggtcgccg
     3361 tgctcatgct gctcggagtt ggcacgcggc ggctttgggc gcgcgacagc ggccgcgccc
     3421 gactgtacgc gcacgccgtg ctctgcatgc ttacgatggg gacgctctca gcgattcctg
     3481 tgttcgggct ctacgtgccg agcatgcgct atctgggcga cttcacgccg ggccaggcgc
     3541 tgctcggatt gatgggtggg ctcggattgt atgcccgcgt gcgcacgcac cccggaaagc
     3601 gtcgcgcggt cggcgcgctc gtggtcacgc tctgcgtggc gac
//
LOCUS       DASTKU010000029        17288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_673935, whole genome shotgun sequence.
ACCESSION   DASTKU010000029 DASTKU010000000
VERSION     DASTKU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 17288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17288
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673935"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFQ61_00885"
     CDS             <1..267
                     /locus_tag="VFQ61_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953021.1"
                     /translation="TGLLSIRTLMRPAPRLEAVWPLSIALHKLVQTGESVGVVMDGDH
                     PLGLLEHAQVLSKLAQTPRVDWPKASASARNEREFSSSESPHAS"
     gene            327..1172
                     /locus_tag="VFQ61_00890"
     CDS             327..1172
                     /locus_tag="VFQ61_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953022.1"
                     /translation="MNSPSASSAPPESQLTADRPPRARFKPSYYAVLAAACALAIWQL
                     KLAPFLLVGAFIARVALSRDEARATYALSWLSMGLALVAFVRFLLLEAVPGIVQGGTR
                     ATQDAAVSKLREILAAEDALRRNAQWDPDHDGVGSAARLDELTGRKPIRGLRSLEAPL
                     LERYPAPVETAIGPAVELGGYLFAVCLPTSEGGFSARLDAAIDDELAERRFLAYAWPA
                     RAGQGLNQTFFIDEHENIRKTSSARYIGASGAPDCNAALGASSEWQDWKHKRPRRTLP
                     GDHPR"
     gene            1194..2384
                     /gene="dusB"
                     /locus_tag="VFQ61_00895"
     CDS             1194..2384
                     /gene="dusB"
                     /locus_tag="VFQ61_00895"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET9953023.1"
                     /translation="MNSNAATYVSDYNREVPPAREGEFTELKIGPLRVWPPVVLAPMA
                     GVTDYPFRAICRQYGAGLYVSEMITCRPLVEGNSKTLKLASFGPDESPRSLQLYGVDP
                     YYVGEAVKRLVGEGLVDHIDMNFGCPVRKVTSRGGGAALPAKPKLLAQIVRAAVQNAG
                     KIPVTIKFRKGIQPGWLTYQRTGRIAEEEGCAAVGLHARTAAEFYSGHADWSAITELK
                     GALRIPVLGNGDIWEAADALRMMRETGCDGVIVGRGCLGRPWLFRELATLFDGRALTP
                     PPHLGEVADVMVQHAGRLCAWFGEGPAMRGFRRHATWYTKGFKGSAELRDRLMRMETL
                     RDLEEILAKIDRTQAFPVSALRVPRGKTGSTQQVSLPHNYCDDPEDATIPEESEDDAF
                     SEGG"
     gene            2522..3244
                     /locus_tag="VFQ61_00900"
     CDS             2522..3244
                     /locus_tag="VFQ61_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953024.1"
                     /translation="MRVARLFTEIASWRWAPAVGLFGGAVVYVALAASVIPDRLELEV
                     KEGAAHRNVGASFAASFGQSTHADTRIVDTHVGSSTPFDESQARREAPTPVRAQHAPA
                     RRGFSPPLPRPDPPPPPPAPVAPPQPVAAPIPPPAPELPPPPSPAAAPEAAAAPAILP
                     APVPVNAAVGNPAVGDGSNAPGGANSPNGSNNSQNGSGNPSNSPGGPPETVPGTPPGS
                     VPGANVPPPRSEPVPGTPPPPG"
     gene            3383..4477
                     /locus_tag="VFQ61_00905"
     CDS             3383..4477
                     /locus_tag="VFQ61_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953025.1"
                     /translation="MDISGEAARFNCAKGARRRVAGAPLLFTLLAASACTVKDKQDLQ
                     FTNGGTEAAGGRAAMSGATGMSGATGMSGAGSGGGELPGSGAGANSGSASGGNPVSGA
                     NGGHTGMDTSAHQGGLSGTTTSGANQAGTTAQGGTSAPPSSTGGVIGTPGGAPNGGSP
                     TGAGGNSNTGGTQAVSAGAAVGNGCDQQLLKNAGFEDGNTQWKLETNFAGVTAIVRKD
                     DAGLVEEGVAPHAGQYLAWLGGIPDNKFDSHYIALTQVFRIPADANLLRLRGYVRIAT
                     QEPDPEMTYDRAFIQMEYPDDREDLLWLPVEWTIKNASRDWIAFDATLEDTSKVRGQD
                     VMLRLQSETDPDLVTSFWVDSLELTARCGR"
     gene            4607..6346
                     /locus_tag="VFQ61_00910"
     CDS             4607..6346
                     /locus_tag="VFQ61_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9953026.1"
                     /translation="MHPVRSLSRFVRPYRSTAVATLSLLTILVALDLAIPRMIQHLID
                     HGVSRGDMPTVITTALGMLALSGLSAVIAVFNNSLSVRVGESVARDLRAALFDRIQGF
                     SFGNLDREKTGALMVRLTSDVATLKGLTQISLRIGTRAPLLMTGSLCLLVVTSPSLAL
                     HMAPLLIVTGLLIAFFVARMEPIFRAVQARLDRLNTTLQENLAGVRLVRSLVRRDFEE
                     GRFQRDNRDMTEQSIRALEFAGAMTPALTLAINVGIVIVLWSGGLSAIRGALSVGQIV
                     AFTNYLLTTLSPLVMMTLFSNTWAMGVVSARRVEDLLATEPEVRDAPDAQSITREQRP
                     EIAFEAVSFSYPGEHNEPALCEVSFQARPGETVAILGATGAGKSTLIALIPRFYDPQA
                     GVVRFGGMDVRRVTQASLLARIAYVPQEAVLFSGTVRDNIRYGRPDATQAQVERAARA
                     AQAHEFIAALPGGYDTRVEARGVNFSGGQKQRLALARALVLDADVLILDDVTSAVDAD
                     TEGRIQQQLAASRGERTTFLVAQRIASVLWADRILLLDKGRLVASGTHAELLRANDLY
                     REIYDSQLGGGTP"
     gene            6343..8172
                     /locus_tag="VFQ61_00915"
     CDS             6343..8172
                     /locus_tag="VFQ61_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9953027.1"
                     /translation="MNERQESATGVRTTFQAGAPRSLGSKLEGAKNPRRALQRGLAYI
                     APEARRLAWVVVFVIGYTVLGLLGPYWMGRGIDRHLMQRDGAGLARIALLMLGAFGCA
                     ALLQLVSNAWMVRISQAAIARLRQDLFTKLQTLPISFFDRNPAGELMSRLTNDLDAIQ
                     QAIAQNLILLFASVLSLVGILVAMFALDGRLALGALLVVPLMVIVTRFVARTTRRGFR
                     ELQQSLGGLNALLEETLSGQRVIRALRRQDAMRSTFQARNTEVFTTGVSANTYALLLM
                     PLTTVLGNLFVIVIAALGGLLALRGLVSVGVIASFVNYGQNFVQPLRQLSNLYNTLQA
                     GLAGAERAFQILDLPSETDAPAERLGPAPKLRGDVRFENVTFGYSPDHPVLHEISLSV
                     RAGAKIAVVGPTGAGKTTLMNILTRFYEVDSGAVYIDGQDLRSIPKATLRLGLGLVLQ
                     DTFLFSASVLENIRYGRLNATDEEVIRAAQLADADYFIRQLPEGYRTQLSERAQNISH
                     GQRQLLSIARTLLADPAILILDEATSSVDSRTEARLQRSLRSLLHGRTSFVIAHRLST
                     IRDADQVLVVDSGRIVQRGSHEELLRQDGPYRGLYLSQFVGKR"
     gene            8207..9409
                     /locus_tag="VFQ61_00920"
     CDS             8207..9409
                     /locus_tag="VFQ61_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9953028.1"
                     /translation="MVATRSLVAVSTGAVLALLTTLLACKRSAGAPDPTPSAPPGAAA
                     AQRPSGSPLPLATLPPSNLVPPSNVQPLSAFAPIEDERNTISVFRETAASAVFVTQTQ
                     VVVDYWAGQRMEVPAGSGSGFVWDKEGHVVTNFHVIQNARSLTVTLQDQSTLEATVVG
                     AEPRKDIAVLKVNAPADKLVPIRLHDPKAFRLEVGQKAIAIGNPFGLDHTLTVGIISA
                     LNRAVDGVGGVQIRDMVQTDAAINPGNSGGPLLDSGGRLIGMNTAIFSKSGVSAGVGF
                     AVPASTISRIVPQLIRTGRAEQVGIGINIDPSGNLERRAGIRGVIVLSVPDGSAAAKA
                     GLRGVERSRRGLSLGDVIVGIDQAPINDYDDLYNTLDKYQAGQKVKLKVRRENQTVEL
                     TVELKPIQ"
     gene            complement(9438..10172)
                     /locus_tag="VFQ61_00925"
     CDS             complement(9438..10172)
                     /locus_tag="VFQ61_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953029.1"
                     /translation="MFSLYAKVAVLAARRAVQSWPVALSLLVYAVILLVASQLTRPLG
                     ILGGMVMGLVLAACWSSYLELISRAIEGGRFRVSWEDFQKTFGARFWDVVSVMFAFWL
                     LSLLTYPLTAGAKGPAFAAITGFAIAFFFNVVPELLYQGSARSFLLLMESARFMLDHA
                     PVWLLPNLLFAAAWLGAAGALDVRHPVELLILFGNTFSSPLQIAQVFGRLPLWALPIE
                     LIGLHYVMVFRGLLFRALASGRRQVY"
     gene            complement(10360..11928)
                     /locus_tag="VFQ61_00930"
     CDS             complement(10360..11928)
                     /locus_tag="VFQ61_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9953030.1"
                     /translation="MKANVLRGFCPEPSQRVMYLRRVVRSAAYVSLLALVLGCSSNDT
                     PSASGAGAAGSGGAGGSHTGGAAQTSGGQGNSGGPSAGGQTAPGGGFGTGGLTQGTSG
                     PSGGSSSVSNGGTPPQAGASPVSGGSASSSGGLSGSGGAMSSGGNGAAGGSNGGSAGG
                     MAAGGATAGGNSNANASVIAAGVRWVGRVDVSDPSTPKFAWSGVGFVATVAGTEIAVK
                     LKSEGGGSDPVFFQPVIDGTPKPRLSVTGSDGVKTVVLGTALAAGDHLVELYRETEGK
                     ANFASSAFLGFASGVPKEPPPYSGRLIEIIGDSISAGYGNLGSEQHPNAGADPSGGCH
                     FTTETESAYMTYGAVAARALRADASILAASGWGIYSDNGGNTNNVLPKIWGNALGERA
                     TPAWSFSQRPQAVVINLGTNDFSANMALGKDPFTTAYRALLKDVRAKYPEAWVYCAIG
                     PMLYGDGLKNATSYITALVQDLNAQGDAKVKLLNFGQQDASKGTGCDWHPNVAEDQRM
                     ADMLKRELSSALGW"
     gene            12477..13874
                     /locus_tag="VFQ61_00935"
     CDS             12477..13874
                     /locus_tag="VFQ61_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HET9953031.1"
                     /translation="MRTTALHFCIFGTILAAAACSAAEGGPIIGTGGSVAPGGGTGAG
                     GASGASTVATGGVTPTGGTRSTGGANTGGVVSTGGGGTTSTQPVAGGGSTAVSGGATS
                     VSGGATSVSGGASTTGGRPGGGAPGSGGASSGGRPNTGGASSAGASSGGTTSSTGGMT
                     ATGGGTSQPPPGKKFVGNITTGRTNMDSGGKTFSKYWDQVTPENAGKWGEVQSSATAS
                     PNWSTLDQIYDYTQKNNIIFKQHTFIWGAQQPSGNITEANVKKWMKDFCTRYPNTKLI
                     DVVNEPPPHTRPGYRNSIGGGDDSTWQWITNAFKWAREACPNAILILNDYNNIEWDND
                     NAHFISIVQKIKEGGAPIDAVGCQAHDLDVNDKAMDLSKVKTLVENLHNKTQLPLYIT
                     ELDLTNTDDQTQLQWYKQFFPYFYGLDYVHGITIWGWIYGQTWSLSPNSGLVRNGSFR
                     PAMTWLMGELGRPTN"
     gene            complement(14067..15152)
                     /locus_tag="VFQ61_00940"
     CDS             complement(14067..15152)
                     /locus_tag="VFQ61_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953032.1"
                     /translation="MAWFRQKATRASLGFLPLRGHVWGAFALTLVIGAACNRKTESER
                     ASVLDNAAASAQPVSSSELPPAPENPVAKVNPYETVESTKLLELPVSAFRATLGLDDE
                     AIFLLTADTAYRLVPGQPPRQFPLDLGYGATVMRSSILFWSKQAIWKANKQDGKARRI
                     AKLPQQPQYFVSSGDDFAWIERNEQEGRYRVSSLDGTKPRVLYESANELIAAAMLNDW
                     VFFVDRPDDASWRVGAVRTGGGAPVFSAPKTGRTPSMLAAWRDIYLYEGATRNVLKLS
                     PDLRHEETVIRDFVCSPLAVFQNVYCAHVEGLSAISPSDPTPQRVIDNVWGAISRIAA
                     NANRVVWLNDAGQDKLALRMIELPNRK"
     gene            15262..16230
                     /locus_tag="VFQ61_00945"
     CDS             15262..16230
                     /locus_tag="VFQ61_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HET9953033.1"
                     /translation="MLAIRPNEPGGPEVLQLRELPTPAPGGGQLLVRTEAIGVNYIDV
                     YHRTGLYKLDSPLRLGQEGAGIVESVGSGVKEVKVGDRVAWCSGPGSYATHVILDEAR
                     VVPVPAGIDPQLAGALLLQGLTAHYLAVDTFATSAEHTVLVHAAAGGVGLLLVQIARR
                     RGARVLATVSTPEKAELARAAGAEHVILYTDQDFSTEVRKLTAGAGVHAVYDSVGKST
                     FEKSLRSLRPRGMMVLYGQSSGPVEKIDPQILAANGSLFLTRPTLGHYTASREELLSR
                     AQDLLTWVQSGHLTVRIGATFPLSEARAAHEALEARKTTGKVVLLP"
     gene            complement(16367..17014)
                     /locus_tag="VFQ61_00950"
     CDS             complement(16367..17014)
                     /locus_tag="VFQ61_00950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9953034.1"
                     /translation="MMQWKRYAETPAFVREHFQKQAVSFDGLYEDDSLLQRTIRPGLF
                     QRRKAAMDLVRRCDHPLVLDVGCGSGRVAESILEVGVKGYVGVDFSEPMLELAGQRLQ
                     RFGQLVTLRQGNFLELPFDTTFDVILGLGLFDYVEEPDRFTRRMRELSRGWVYATFPR
                     WNWYKGVVRHIRYRVINRCPIYDYTERELRFLFKGSGFDDVQIDVRRNGFAVLAR"
BASE COUNT         3283 a         5519 c         5437 g         3049 t
ORIGIN      
        1 acggggctgc tctcgattcg gaccctgatg cgcccagccc cgcggctgga agccgtctgg
       61 cctctgagta tcgctcttca taagctggtg cagacaggcg agtccgtcgg cgtggtgatg
      121 gacggcgatc accctctcgg actactcgag catgctcaag tgctttcgaa gcttgcccaa
      181 acgccccgcg tggactggcc caaggcttcg gcaagcgctc ggaatgagcg agagttcagc
      241 tcttccgaga gccctcatgc gagctgagtt tgcgcccgcg cgccgggtga ggcattgcgg
      301 cgcccgagac tcgaggcact gccgccatga attccccgtc cgcttcatca gcgccccccg
      361 aatcccaact gaccgctgat cgaccgccgc gggcccgctt caagccaagc tactacgccg
      421 ttctggccgc cgcatgcgct ctggccatct ggcagctcaa actggccccc ttcctattgg
      481 taggcgcttt catcgcgcgg gtggctttat cgcgcgatga agcgcgagcg acctacgccc
      541 tgagttggct gagcatgggg ctcgcgttgg tcgccttcgt gaggttcttg ttgctggaag
      601 ccgtcccggg aatcgtacaa ggagggaccc gggcgaccca agacgcagcg gtctcgaagt
      661 tgcgcgagat cctggccgcc gaagacgcat tgcgccgcaa tgcacagtgg gaccccgatc
      721 acgacggggt gggctcggca gcgcgcctgg atgagctcac cgggcgcaag ccgatccgcg
      781 gcttacgttc gctcgaagcc cccttgctcg agcgctatcc cgcgcccgtc gagaccgcca
      841 tcggccccgc cgtcgaactc gggggttatc tgttcgcggt ttgtctgccg acttccgagg
      901 gtggtttcag cgcgagactc gacgctgcca tcgacgacga gcttgctgag cggcgcttcc
      961 tggcctacgc ctggcccgcc cgcgcaggcc aaggcctgaa ccagacgttc ttcatcgacg
     1021 agcatgaaaa catccggaaa acgtcgtccg cccggtacat cggcgcttcg ggagcacccg
     1081 actgtaatgc tgcgctcgga gcctcgagcg agtggcaaga ttggaagcac aagcgcccgc
     1141 gccgtacgtt gccgggagat cacccgcgct gaatcgcccc cgacaagccc gcaatgaact
     1201 cgaacgcagc gacctacgtc agtgactaca accgtgaagt tccgcctgcg cgagagggtg
     1261 aattcacgga gttgaaaatc ggccctctcc gcgtctggcc gccggtagtg ctggcgccca
     1321 tggcgggggt cacggactat ccatttcgtg caatttgtcg ccaatacggc gccggtctgt
     1381 acgtgagtga gatgatcact tgccgccccc tcgtggaggg taacagcaag actctcaagc
     1441 tcgcgagctt cggccccgac gagtcgcctc gcagtttaca gctatacggc gtcgatccgt
     1501 attacgtcgg cgaggccgtg aagcgcctgg tcggcgaggg cttggtcgat cacatcgata
     1561 tgaacttcgg ctgccccgtg cgcaaagtca cgtcgcgggg cggcggcgcg gcgctgcccg
     1621 caaaaccgaa actgctcgcc caaatcgtgc gcgcggccgt gcagaacgcg ggaaagatcc
     1681 cagtcacgat caagttccgc aaaggcattc agccaggctg gctcacttac cagcgcaccg
     1741 gacgaatcgc ggaggaagaa ggctgcgccg ccgtcggttt gcacgcccga accgccgcgg
     1801 agttctattc gggacacgcg gattggtccg cgatcacgga actcaagggc gcgctcagaa
     1861 tcccggtgct cgggaatggg gatatctggg aggccgcaga tgcgctgcgg atgatgagag
     1921 agacaggctg cgacggagtg attgtggggc ggggttgttt ggggcggcca tggttgttcc
     1981 gcgagctcgc cacactgttc gacggtcgag cgctgacccc tcctccccac ttgggcgaag
     2041 tcgcggatgt catggtgcaa cacgcaggga ggctttgcgc ttggtttgga gagggtcccg
     2101 ccatgcgcgg cttccgcagg cacgccacgt ggtacacgaa gggattcaaa ggcagcgcgg
     2161 agttgcgtga tcgattgatg cgcatggaga ctttgcggga cctcgaagag atcctcgcca
     2221 aaatcgatcg cactcaggct tttcccgtct cggctctgcg cgttcctcga ggtaagacgg
     2281 gcagtacaca acaggtgagc ctgcctcaca attattgcga cgatcccgaa gacgccacga
     2341 tccccgagga atccgaagat gacgcatttt cggaaggcgg ctgaaaccaa cacgagaacg
     2401 tgtctcctcc ttgcctaatg agcagggcgt cggccctgcg gggcgagaac gtcacggcgg
     2461 caggtttcga gaaaacaact gggcactcgc cgctggctcg ggctagactt ctccggggcc
     2521 gatgcgagta gcgaggcttt ttacggaaat tgcgagctgg cgttgggcgc ccgcggtagg
     2581 actattcggc ggggccgtgg tctacgttgc gctcgccgcc agcgtcattc cggatcggct
     2641 cgagctcgag gtcaaagagg gtgcggccca cagaaatgtt ggcgcatctt tcgccgcgag
     2701 cttcgggcag agcacgcacg cagatactcg gatcgtcgat acgcacgtgg gctcgtctac
     2761 gccgttcgat gaatctcaag cgcggcgcga ggcgccaact ccagtccgtg cgcagcatgc
     2821 gcctgctcgt cgtggcttct cgccgccgct gccacgaccg gatccaccgc cccctccccc
     2881 cgcaccggtt gctccgccgc agccagtggc cgctccgatc ccgccaccgg cgcctgaact
     2941 tcctcctccg ccgtcgcccg ctgccgcgcc cgaagcagcc gccgcgcctg cgatactccc
     3001 cgcgcccgtc ccggtgaatg ccgcggttgg taaccctgct gttggagacg ggagcaatgc
     3061 ccccggcggg gccaactcgc ccaatggctc caacaattct cagaatggat cgggcaatcc
     3121 atccaacagt cccggcggtc cgcccgagac ggtaccggga acaccgccgg ggagtgtgcc
     3181 cggggcgaat gttcccccgc ctcgatccga acccgtgccg ggcacgccgc caccaccggg
     3241 ctagtcgaac acagccgcgc gctcgaaatt cgatattcga attcacatcg cgaatcagct
     3301 tgggatttgc gtgcacacag acaatcgaca ccgttcgcga cgtgcaagac gaacccgggc
     3361 cgtgtgctag taatcgcctg gtatggatat ctcgggagag gcagctcggt tcaactgcgc
     3421 caagggggca agaaggaggg tggctggggc gccgctccta ttcactctac ttgccgcgtc
     3481 cgcctgcacc gtcaaagaca agcaagatct tcagttcacc aacggcggca ccgaagcagc
     3541 ggggggccgc gcggccatga gcggagcgac gggcatgagc ggagcgacgg gcatgagcgg
     3601 agcaggttcg ggcggcggag agctcccggg cagcggcgcg ggcgcaaaca gtggctccgc
     3661 gagcggtgga aacccggtga gcggcgcaaa cggcggtcat accggcatgg acacctcggc
     3721 tcaccaaggc ggactgagcg gcactacgac atcgggagcg aaccaggcgg ggaccactgc
     3781 ccaaggcggc acttccgcac ccccgagttc aaccggagga gtgattggta cgccgggcgg
     3841 cgccccgaac ggtggcagcc caactggcgc cggcggcaac tcgaatacgg gcggaaccca
     3901 ggccgtcagc gcgggcgcag ctgtcggcaa cggctgtgat caacagctgc tcaagaacgc
     3961 cggcttcgag gacggcaaca cacagtggaa gctcgagacc aacttcgccg gcgtgacggc
     4021 gatcgtaaga aaagacgacg cgggcctcgt cgaagaagga gtcgcgcctc acgccggaca
     4081 atacctcgcg tggctcgggg gcataccgga caacaagttc gattcgcatt acatcgcgtt
     4141 gactcaggtc ttcagaattc cggcggacgc gaacctgctg cgactgcgcg gctatgtgcg
     4201 tatcgctact caagagccgg atcccgagat gacttacgat cgggcgttca tacagatgga
     4261 gtatccagac gatcgtgaag atctcttgtg gctaccggtc gaatggacca tcaagaatgc
     4321 gagccgcgac tggatcgcct tcgatgccac gctggaggac acgagcaaag tccgcggaca
     4381 ggacgtcatg ctgcgcctcc aatccgagac cgatccggac ctggtcacga gtttctgggt
     4441 ggattcgttg gagctgaccg cgcgttgcgg tcgctgatgg gagcgggttc tttcccccgc
     4501 agcgcgtcag acgggccgga tacagagcgg acagcctgcc cgggtcgaga gcctgagctc
     4561 gggctgcctt gggctcacgc cgcaaaacca atacactgcg ccggacatgc acccggtgcg
     4621 ctcgctctcg cgcttcgttc gcccctaccg ttcgacggcc gtcgcgacgc tgagccttct
     4681 cactatattg gtcgcgctgg atctggcaat tccgcgcatg attcagcact tgatcgatca
     4741 tggggtgagc cgcggcgaca tgcccaccgt gatcacgacc gcgctcggta tgctcgcgct
     4801 ttcgggactc agcgccgtga tcgcggtgtt caacaactcc ctctcggtcc gggttggcga
     4861 gagtgtcgcg cgcgacctcc gggccgcctt gttcgacaga atccagggct tctcgttcgg
     4921 caatttggac cgagagaaga cgggcgcgct gatggtaagg ctgacgagcg acgtggcgac
     4981 gctcaagggg ctcacccaaa tctccctgcg catcgggacc cgcgcgccgc tcttgatgac
     5041 gggaagtttg tgcctattgg tcgtgacgag cccgagcttg gcgttgcaca tggcgccgct
     5101 gctgattgtc acgggcctgc tcatcgcctt cttcgtggcg cgcatggagc ccatctttcg
     5161 ggccgtgcag gcacgactgg accggctcaa tacgacgctg caggagaatc tagccggcgt
     5221 tcggctagtg cgttccttgg tgcgccgcga tttcgaggaa ggccgcttcc agcgcgacaa
     5281 tcgggacatg accgagcagt cgattcgcgc gctggagttc gcgggcgcga tgaccccagc
     5341 gctcacgctg gcgatcaacg taggcatcgt catcgtgctt tggtcagggg gactgagcgc
     5401 gatccgcgga gcgctcagcg tcggacaaat cgtggctttc acgaactacc tgctcacgac
     5461 gctgtcaccc ctggtcatga tgactctgtt ctcgaacaca tgggccatgg gcgtcgtctc
     5521 ggcccggcgt gtcgaggact tgctcgcgac cgaacccgaa gtgcgcgacg cgcccgacgc
     5581 gcaatccatc acccgcgagc agcgccccga gatagcgttc gaagcggtca gcttcagcta
     5641 tcctggtgaa cacaacgaac ccgcgctgtg cgaggtcagt tttcaggccc ggccgggcga
     5701 gaccgtcgcg attttgggcg ctactggcgc cggcaaaagc acgctaattg ctctcattcc
     5761 gcgcttctat gacccgcaag ctggggtggt gcggttcgga gggatggatg tgcggcgcgt
     5821 gacccaagcc agcctactcg cgcgcatcgc gtatgtaccc caggaagccg tgctgttctc
     5881 gggcacggtt cgcgacaata tccgctacgg gcgccccgat gcaactcagg cgcaagtcga
     5941 gcgcgccgcg cgagcagccc aggcccacga gttcattgcg gcactgccgg gtggttatga
     6001 cacgcgcgtg gaggcgcgcg gcgtgaactt ctctggcggt caaaagcaga ggctcgcatt
     6061 ggcgcgggcc ttggtgctcg acgcggacgt tctgatcctc gacgatgtca ccagtgccgt
     6121 ggacgcggac accgaaggcc gcatccagca acagcttgcc gcctcccgcg gcgagaggac
     6181 gacgttcttg gtcgcgcaga gaatcgcgag cgtgctctgg gcggaccgca tcttgttgct
     6241 cgacaagggg cgcctcgtcg cctcgggcac tcatgccgag ctgctacgag cgaatgatct
     6301 ctatcgcgag atctatgact cgcagctcgg cggaggtacc ccgtgaacga gcgccaggaa
     6361 tcggccacgg gggtgcgcac gacgtttcaa gcgggcgccc ctcgcagcct gggctcgaag
     6421 ctcgaaggcg caaagaatcc acggcgcgct ctgcagcgag gtctcgcgta catcgcccca
     6481 gaagcgcggc gcctcgcgtg ggtggtggtg ttcgtgatcg gctataccgt gctcggcctg
     6541 ctcggtccat attggatggg gcgcgggatc gatcgtcatt tgatgcagcg ggacggcgcg
     6601 ggcttggctc gcatcgcgct gctcatgttg ggtgcgttcg ggtgcgcggc gctgctgcag
     6661 ctcgtgagta acgcctggat ggtacgcatc tctcaggcgg ccatcgcgcg gttacgccag
     6721 gacttgttca cgaagctgca aacgttgcca atctccttct tcgatcggaa cccggcgggg
     6781 gagctgatga gtaggctcac caacgacctg gatgcgatcc agcaggccat cgcgcagaat
     6841 ctgattctgc tgttcgcgag tgtgctctcc ctggtcggta ttctcgtagc gatgttcgcg
     6901 ctcgatggtc ggctcgcgct cggggcgctg ctggtggtgc cgttgatggt gatcgtgacc
     6961 cgcttcgtcg cgcgcaccac ccgccgagga ttccgggagc tccagcaaag cctggggggt
     7021 ctcaacgcgc tgctggagga gaccctgagc gggcagcgcg tgatccgggc tttgcgccgc
     7081 caggacgcca tgcgatcgac ctttcaggcg cgcaacacgg aggtgttcac gacgggcgtg
     7141 tccgccaaca cctacgcgct attgctgatg cccttgacga cggtgctggg caacttgttc
     7201 gtgatcgtga ttgcggcgct gggcggcctg ctggcgctgc gcggactcgt aagcgtcgga
     7261 gtcatcgcga gcttcgtcaa ctatggccaa aatttcgtac aaccgctaag gcagctatcg
     7321 aatttgtaca acacgctaca agccggattg gccggcgcgg agagagcatt tcagattctg
     7381 gatttgccca gcgaaaccga tgctccagcc gagaggctcg gcccagctcc caagctccgg
     7441 ggtgacgtcc gattcgagaa tgtcacgttc ggatatagcc ccgaccaccc cgttttgcac
     7501 gaaatctccc tgtccgtccg agccggcgcc aagatcgcag tcgtgggccc gacgggggcg
     7561 ggcaagacca ccttgatgaa catcctgacc cgcttctacg aagtggactc gggggcggtc
     7621 tacatcgatg gccaggactt gcgatccatt ccaaaggcga cgctgcgcct ggggctcggc
     7681 ttggtactcc aagacacctt cttgttctcg gccagcgtac tcgagaacat tcggtacggg
     7741 cgcctcaacg ccaccgacga agaggtgatc cgcgcggcgc agctggccga tgcagactac
     7801 ttcattcgcc agctccccga gggctatcga acccagctct cggagcgtgc ccagaatata
     7861 agccacggac aacgccagct gctcagcatc gcgcgcacct tgctcgccga ccctgcaatc
     7921 ttgatcttgg acgaagccac gtccagcgtc gattcgcgca ctgaagcgcg cttgcagcgc
     7981 tcgctccgta gcctgttgca tggacgcacg agcttcgtga tcgcccatcg gctgagcacg
     8041 atccgggacg ccgaccaggt gttggtagtg gatagcggcc gcatcgttca gcgcggaagc
     8101 cacgaagaac tactccggca ggatggccct taccgcgggc tctacctcag tcaatttgtg
     8161 ggcaaaaggt agtgtacgcg gcggccgtcg cggtatgctg ccgcgcatgg ttgcgactcg
     8221 atctctggtt gcggtttcca ccggcgccgt gctggcgctg ctgacgacac tgctcgcatg
     8281 caagcgctcc gccggcgcgc ccgacccgac cccctctgct ccgcccggcg cggccgccgc
     8341 gcaacgtccg tcgggcagcc ctctgccgct ggcgacgctg ccgccatcca atctggttcc
     8401 gccttccaac gtgcagcctt tgtccgcgtt tgcgccgatt gaagacgagc gcaacaccat
     8461 ctcggtcttt cgagagaccg cggcttccgc cgtgttcgtg acgcaaaccc aagtcgtggt
     8521 ggactattgg gcgggccaac gcatggaggt gcccgccggc tcgggctcgg gcttcgtgtg
     8581 ggacaaagaa gggcacgtgg tcacgaattt ccacgtcatt cagaacgcgc gtagcttgac
     8641 ggtgacgctg caggatcagt ccacgctgga ggccacggtg gtgggtgcgg agccgcgcaa
     8701 agacatcgca gtgctcaagg tgaacgcgcc cgccgacaag ctcgtgccca ttcgtcttca
     8761 cgatcccaag gcgtttcgac tggaggtggg gcagaaggcg atcgcaatcg gtaacccgtt
     8821 cgggctcgat cacacgctta ccgtgggcat catcagcgcg ctcaaccgcg cggtggatgg
     8881 cgtaggcggc gtacaaatcc gcgacatggt gcaaaccgac gcggccatca accccggcaa
     8941 ctccggcggc ccactgttgg attctggtgg tcgcttgatt gggatgaata ccgccatctt
     9001 ctcgaagagc ggcgtctcgg cgggcgtggg cttcgcggtg ccagccagca ccatctcgcg
     9061 catcgttccg caactgattc gcacggggcg cgccgagcaa gtcggcatcg gaatcaacat
     9121 cgatccgagc gggaatctcg aacgccgtgc cgggatccgg ggtgtgatcg tgctgagcgt
     9181 gcccgatggt agcgcggcgg cgaaggccgg gctgcgcggc gtcgagcgct cgcgccgcgg
     9241 gctctccctg ggcgacgtga tcgtcggtat cgatcaagct ccgatcaacg actacgacga
     9301 tctgtacaac accctcgaca aataccaagc gggccagaag gtgaagctaa aggtccgcag
     9361 agagaatcag acggtcgagt tgacagtcga gttgaagcca atccaatagc tctccaacgc
     9421 tggccgagga gagcgcctca gtaaacctgt cgacggccac tggcgagggc tctgaacaag
     9481 agccctcgaa acaccatcac gtagtgcaag ccgatcagct cgattggcag cgcccatagc
     9541 ggcaatcgcc cgaagacctg cgcgatctgc aaaggcgagg agaaggtgtt tccgaacaag
     9601 atcaggagtt cgaccgggtg ccgcacgtcg agcgcgccgg ccgcgccgag ccaagccgcg
     9661 gcgaacagca ggttgggcag cagccagacc ggcgcgtgat ccaacatgaa gcgcgccgac
     9721 tccatcagca gcaagaaaga gcgtgcgctg ccctgataca gaagctcggg aacgacgttg
     9781 aagaagaatg cgatcgcgaa gcccgtgatg gccgcgaagg caggcccctt ggctcccgct
     9841 gtcagcgggt aggtcaggag agacagcagc caaaacgcga acatcacgct gaccacgtcc
     9901 caaaagcgcg cgccaaaggt cttctggaag tcctcccaag aaacacggaa gcgtccgccc
     9961 tcgatggctc tagaaatcaa ctccaggtaa ctcgaccaac acgccgccag aacgagcccc
    10021 atgaccatgc cgcccaagat gcctagaggc cgggtcagct gcgaagcgac cagcaggatt
    10081 actgcgtaca ccaacaacga gagagcgacg ggccagctct gcaccgcacg acgagcggcc
    10141 aacacggcga ccttggcata gagggaaaac atctgaccgt atccccataa gccgattcga
    10201 ccgttagtcc agagcgaacc gttccggacg ctcagcagaa cggcttttcc gccgatcggc
    10261 tggcagctcg ggaaatccga agctgggcgc gcgagtgagc tagaggggcg cgagatgtcg
    10321 cggcgtctct agctcacatc gctcgagctc ctggtagact caccagccca aggccgagga
    10381 cagctcgcgc ttcagcatgt ctgccatgcg ttggtcttcg gcaacgttgg ggtgccaatc
    10441 acaacccgtc cccttgctcg cgtcctgttg accgaaattg agcagcttga ccttggcgtc
    10501 cccctgcgcg ttcaagtcct gcaccaaagc cgtgatgtaa ctggtggcgt tcttcaagcc
    10561 atctccgtac agcatcggac cgatcgcgca atacacccaa gcctctgggt acttggcacg
    10621 cacgtctttg agcaaagccc ggtaggcggt cgtaaacgga tccttaccga gcgccatgtt
    10681 cgccgaaaag tcgttggtcc ccaaattgat gaccaccgcc tgggggcgtt gcgagaacga
    10741 ccaagcagga gtggcgcgct ctccgagtgc attgccccaa atcttcggta agacgttgtt
    10801 cgtgttcccg ccgttatcgc tgtagatgcc ccagcccgac gccgcgagaa tactcgcgtc
    10861 ggcgcgcaga gcgcgtgcgg caactgcccc gtaggtcatg tacgcgctct cggtctcggt
    10921 ggtgaagtga cagcctccgc tcggatccgc acccgcgtta gggtgttgtt cactcccgag
    10981 attgccgtag cccgcgctga ttgaatcacc aatgatctcg atgagacgac cggagtacgg
    11041 gggtggctcc ttcggcacac cgctcgcaaa tccgaggaac gcactggagg cgaagttcgc
    11101 tttgccctcc gtttcacggt agagctcgac caaatggtca ccggcggcga gcgcagtgcc
    11161 gagcacgacg gtcttcacgc cgtctgagcc tgtgacggag aggcgcggct ttggcgtgcc
    11221 atcgatcacg ggctggaaga acacgggatc gctgccgcca ccctctgact tgagcttcac
    11281 ggcgatctcg gtgccagcca ccgtggccac gaaaccaaca cccgaccatg caaacttggg
    11341 ggtagatgga tccgatacat ccacgcggcc cacccaacgc actcccgccg cgatcacaga
    11401 cgcattggcg ttcgagttgc cgcccgcggt tgctccacca gccgccatcc ctccggcgct
    11461 gccaccattg ctacctccag cagcgccgtt cccgcccgag ctcatggctc cccccgaacc
    11521 ggagagacca ccgctgctag aggcgctgcc cccgctgacc ggcgaggctc cggcctgggg
    11581 tggcgttccg ccgttcgaga ccgagctcga tccgccgctc gggccactgg taccttgggt
    11641 caaccctccg gtgccgaagc cgcccccggg cgccgtctga cccccagctg atggcccgcc
    11701 ggagtttccc tggcctccgc tcgtctgcgc cgcacctcca gtgtggcttc cgcccgctcc
    11761 tcccgacccc gctgcgcctg cccccgaggc agacggcgta tcgttcgatg agcagccgag
    11821 cacgagggcc agcaacgaga cgtaagccgc cgatcgcacg acccgccgca ggtacatcac
    11881 ccgttgggat ggctcaggac agaatcctcg caaaacattc gctttcaagt cgtttcctcg
    11941 agttcgggtc gtatgctggg ccggcgcagt acggcggttc gcagatttga accgcccttt
    12001 gcttcttagc aactcttagc gaccgcgctc tccgcgacat gcgtctccgc agccgcgaag
    12061 cctagacaac tctacgggct cgaggcgcat gctcacgagc gcaaattggc tcgggttctt
    12121 gcgcaaagcg acgcactgga gacgtgcacc catcgcttgg gctgccacca tcaacgccag
    12181 aatcgaggct gcgttcgcac cccacgctcg actccgaagg tgagctccgc ggggccacgg
    12241 gcctcacctg gaaccggtac cagatcggcg tgcctcacga gacccgtcgc taaacgtgac
    12301 tggaaaattg ctgcttcacg acgaatgttc atggccttgt cgaatcactg acggacctag
    12361 cgagttttgc tcactgctca gtgccagcgt gatcgatcta ctgaaacctg ggcattagtc
    12421 gcctagccaa actagtcgcc tagccaaacg caacgcctat aacctcggag ttcgccttga
    12481 gaactacagc actgcatttt tgtatttttg gcaccatcct ggcggctgcg gcctgtagcg
    12541 ccgcggaagg cggcccgatc attggcacgg gtgggagcgt ggctccgggc ggaggaacgg
    12601 gcgcgggcgg cgcttctggc gcgtctacgg ttgcgacggg cggcgtcacc cccacgggtg
    12661 gtactcgatc gacgggaggc gcgaataccg gtggggttgt gtccacgggt ggaggcggca
    12721 ccacgagcac gcagcccgtc gcgggtggcg gctccaccgc cgtgagcggt ggcgcgactt
    12781 ccgtgagcgg tggtgcgact tccgtgagcg gcggtgcctc cacgaccggc ggtcgcccgg
    12841 gtggcggtgc gccgggcagc ggcggtgcga gctctggtgg gcggcccaac acgggtggcg
    12901 cctcgtcagc tggcgcctcg tcgggcggaa cgacctcgtc aacgggagga atgactgcga
    12961 ccggaggagg tacctctcaa cctccccccg gcaagaagtt cgtgggcaac atcacgacgg
    13021 gccggaccaa catggactcc ggcggtaaga cgttctccaa gtattgggac caagtcacgc
    13081 ccgagaacgc gggcaagtgg ggcgaagtcc agagctcggc caccgcatct cccaactgga
    13141 gcacgctgga tcagatctac gactacacgc agaagaacaa catcatcttc aaacagcaca
    13201 cgttcatctg gggcgcgcag cagccgagcg gcaacatcac ggaggcgaac gtcaagaagt
    13261 ggatgaagga tttctgcacg cgatatccca acaccaagct gatcgacgtc gtcaacgagc
    13321 cgcctccgca caccaggccc ggatatcgca acagcatcgg cggtggtgac gatagtacgt
    13381 ggcaatggat cacgaatgcg ttcaagtggg cgcgcgaagc ctgccccaac gccatcctga
    13441 ttctgaatga ctacaacaat atcgagtggg acaacgacaa cgcccacttc atcagcatcg
    13501 tccaaaagat caaggaaggc ggcgccccca tcgacgcggt aggctgtcag gcgcacgatc
    13561 tggacgtcaa tgacaaggcg atggacctga gtaaggtcaa gacgctagtc gagaatctcc
    13621 acaacaagac ccaactgccg ctctacatca cggagttgga tctcacgaac accgacgatc
    13681 agacgcagct ccagtggtat aagcagttct tcccgtactt ctacgggctg gattatgtgc
    13741 acggcatcac gatctggggt tggatctatg gccaaacctg gagcctatcg ccaaacagcg
    13801 gtctcgtgcg caacggctct ttccggccag caatgacgtg gctcatgggt gagctcggcc
    13861 gtccgacgaa ctgagaactc agcgcgactc gatccaaagg ggtgcaagct cagcttgcac
    13921 ccctttgctt tttgtccgct cacatatccg tcaccctcgc tcaaccgccc cagctgcgag
    13981 atcgatgtgg aggactgcgc ccgcctgccc cgtgagaacg gcgcaagctg cgtgggccgc
    14041 atcggccggc acagcgcttc acccgttcat ttgcggtttg gcagttcgat catgcgcagc
    14101 gccagcttgt cttgccccgc atcgttgagc cacacgacgc ggttggcgtt ggcggcaatc
    14161 cgactgatgg cgccccagac gttgtcgatc acgcgctgtg gagttgggtc cgagggagag
    14221 atcgcgctga gcccctcgac gtgcgcgcaa tatacattct gaaacaccgc gaggggtgaa
    14281 cagacgaagt cgcgaatcac cgtctcctcg tgacgcagat caggagaaag tttcagcacg
    14341 ttgcgagttg cgccttcgta caggtaaatg tcgcgccaag ccgccagcat ggagggggtg
    14401 cgcccagttt tgggcgcgga gaataccggt gcgcccccgc cggttcgaac cgcgcccaca
    14461 cgccaactgg cgtcatccgg gcggtctacg aagaagaccc agtcattgag catggccgcc
    14521 gcgatgagct cgtttgccga ctcgtagagg acgcggggct tggtaccgtc caagctactc
    14581 acccggtagc gcccctcctg ttcatttcgc tcgatccaag caaagtcatc cccggaggaa
    14641 acgaagtact gcggctgctg cgggagcttc gcgatacgtc gcgctttgcc gtcttgtttg
    14701 ttcgccttcc aaatggcctg tttggaccaa aatagaatgg aggagcgcat gaccgtcgcg
    14761 ccatacccca agtccaacgg gaactgccgc ggcggctgcc cgggaaccaa ccgataggca
    14821 gtatcggctg tgagcaagaa tatggcctcg tcatccagcc ccagcgtggc tcgaaaggcg
    14881 gacacgggga gttccaagag cttggtcgac tccacggttt cgtaggggtt gaccttcgcc
    14941 accggattct ccggggcggg cggaagctcc gaggagctga cgggttgcgc agatgcggcg
    15001 gcattatcga gcacgctcgc gcgctcgctc tccgtcttgc gattgcatgc ggcgccaatg
    15061 acgagggtca gcgcgaaagc cccccaaacg tgtccccgga gcgggagaaa tccgagcgag
    15121 gcgcgcgtcg ctttttgacg aaaccaagcc atgtgttgac gatggcgtac aacatcggaa
    15181 ccccttcacg ccagcgctgg gccgagattc tcccgcaaca gggctatggc tccgcgaggt
    15241 gggctgcggt aaaagcgtgt catgctggca atccgaccga acgagcccgg tggacctgag
    15301 gtgttacagc tgcgggagct ccccactcct gcccccgggg gaggccagct cttggttcgt
    15361 accgaagcca tcggagtgaa ctatatcgat gtctatcacc gcactggctt gtacaaactc
    15421 gattcaccgc tgcgcttggg ccaagagggc gccggcatcg tggagagtgt cgggagcggc
    15481 gtgaaggagg tcaaagtggg tgaccgcgtc gcgtggtgca gcggtcccgg ctcgtacgca
    15541 acccacgtca tcctggacga agcgcgcgtg gttcccgtgc ccgcgggaat cgatccgcag
    15601 ctcgcggggg cgctgctact tcaaggattg accgcgcact atctggccgt agacacgttc
    15661 gcaacgagtg cagagcacac ggtgctcgtg cacgcagcgg cgggcggagt tgggctcctg
    15721 ctggtgcaga tcgctcggcg ccgcggcgcg cgggtgctcg cgaccgtctc cacgcccgag
    15781 aaggcggagc tcgcgcgggc tgccggcgcc gaacacgtga tcttgtatac cgatcaggac
    15841 ttctccaccg aggtgagaaa actcaccgcg ggcgcgggcg tgcacgccgt ctacgattcc
    15901 gtgggcaaga gcaccttcga gaagagcctt cgctcattgc ggccgcgcgg gatgatggtg
    15961 ctctatggtc aatcgagcgg gcccgtcgag aagatcgacc cccaaattct ggccgcgaat
    16021 ggctcgttgt tcctgactcg cccgaccctc ggccactaca ccgcatcacg agaagaattg
    16081 ctctcgcgcg cccaagatct actgacttgg gtacaatccg gccacttgac ggtgcggatt
    16141 ggagcgacat tcccgttgtc cgaggcgcgg gctgctcacg aagcgctcga ggcccgcaag
    16201 accaccggca aggtagtgct gctgccgtga gcccgggaat ggcaacctag cggacaggat
    16261 atcttgagtc gatgtccgct aggttgttgc gaccgcgaat gcccttgccg tgtcgatcgg
    16321 caactaggct cctccgcagt cagcgccctt cactaggctg atcggttcaa cgagcgagca
    16381 cggcaaaacc gttgcgtctg acgtcgattt ggacgtcatc gaagccactg cctttgaaga
    16441 ggaagcgcag ctcccgctcg gtgtaatcat agatcgggca gcgattgatc acgcgatacc
    16501 ggatgtgccg aaccacaccc ttgtaccaat tccagcgcgg gaacgtggca tatacccagc
    16561 cgcgcgaaag ctcccgcatt cgccgagtga accgatctgg ctcctcgacg tagtcgaata
    16621 gacccagacc caatatcaca tcgaacgtgg tatcgaatgg cagctccagg aagttgcctt
    16681 gtctcaaggt cacgagttga ccgaagcgct gcagccgttg cccggcgagc tccagcatcg
    16741 gttcggagaa atcgacgccg acgtagcctt tcacgcccac ttctagaatg gactccgcca
    16801 cccggcccga gccacacccc acgtcgagta ccaggggatg atcgcaacgc cgcaccaggt
    16861 ccatggcggc ttttcgacgt tggaaaagcc ccggacgaat ggtgcgctgc agaagcgaat
    16921 catcctcgta caggccatca aacgagacgg cctgcttctg aaagtgttcg cgtacgaagg
    16981 ctggggtctc ggcgtatctc ttccactgca tcatggtatt tcacgactcg aaatttgcgg
    17041 gcggctcgac tcaagcgcgc caggatttgg gttcttccga gctccgagcg cacatcatag
    17101 tagcgcgcgc cgagcctttg gaagaggccc gcgccgcgag gtagcggaga cctcaaccat
    17161 tgtgggtcac attcgtaagg atggaagtaa agagctgccc aatgactgtc ggttttcgcc
    17221 aagctacgct cgatgagccc gaggggcaag gcgcgccagt aacttccccc acccactggc
    17281 aagcgcat
//
LOCUS       DASTKU010000030         2858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1789080, whole genome shotgun sequence.
ACCESSION   DASTKU010000030 DASTKU010000000
VERSION     DASTKU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789080"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFQ61_00955"
     CDS             <1..735
                     /locus_tag="VFQ61_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953035.1"
                     /translation="GAAHPNPVVSIAYRDNRVLLAMSAAASSLPYDFLLKSTGKSDCY
                     GEQFASFPLPPPGLNTAYLIHRALRLNCLTIHYADLWQEVATAEIAQDGFAKSDPRLR
                     SWAHLKTKWRRDSALRTPYERRQALVELDALAALSLGLTVEQLLLIYRVQFPVLQQYE
                     RETFYDQRGKIVFTTNRGLSGVGLERKQWEQIRDASAGEKLPPWAKDAGGAFVPPFDS
                     CDREADMAQAYHHFAARLGIGGQSNA"
     gene            732..>2858
                     /locus_tag="VFQ61_00960"
     CDS             732..>2858
                     /locus_tag="VFQ61_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9953036.1"
                     /translation="MIPSVVGSEIRRALLDYLKTTFRLQDAELEAALFRYLESDETGM
                     FQGPYVDVRLPFRQADERWESSSPLDFGPPFVPYAHQLRAFERLSARDRVPQNTLITT
                     GTGSGKTECFLYPVLDHCRRARQRGELGIKAIVLYPMNALASDQAERLAKLLRDPLLH
                     GVSAGLYVGGKGQHAVRGASHLVDDREILRKSPPDILLTNYRMLDFLLMRPEDGALWA
                     LNGPHTLRYLVLDELHTYDGAQGSDVACLIRRLKARLDIPSGHLCCVGTSATIGSGGA
                     QDPRQLLADFATQIFAEPFSADSLIGEERLTPDEAFLKFESKPAFPYIEDRPDWTRLD
                     ATRYARHEAYLLAQVEAWFGDPRPRGPSLLAESLGAHPFMAKLLRALAGRERRSGPRH
                     VAEVMSAIASEEPSFAELSREQQWLVLSSFVSLIAHARSTDASGERRPFLQVQVQLWI
                     REVHGLLRRVGSGPPAFAWQDDLKTEPGEHWLPLVFCRECGFSGYAAALREGETAFRD
                     APGEIGEVFLHNGNRGRVIEVLGEQHGAERRAGVPDQACQKQLCPRCLSLGADGNCRC
                     DPRGIPTLPVRVHPELDEDNKPGPLRRCPDCQADDALSFLASRAATLSSVAVSELFSS
                     RFNADKKLLAFTDSVQDASHRAGFFASRTFRFALRTVVQALVEHHAGQPLPLASFAAG
                     LIRHWSEQLDEPRAAALLMPPDLVEDP"
BASE COUNT          459 a          953 c          912 g          534 t
ORIGIN      
        1 ggtgccgctc accccaaccc agtcgtctcg atcgcatatc gcgacaatcg cgttctcttg
       61 gcgatgtctg cggcggcatc ttccttgcca tacgacttcc tgttgaagtc tactggcaag
      121 agcgactgtt acggtgagca attcgcttca tttccgctgc cgcctcccgg gctcaatacg
      181 gcctacctca tccaccgcgc cctccgcctc aactgcctca ccatccacta cgccgatctc
      241 tggcaagaag tcgccactgc cgagattgcc caagacggct tcgccaagtc ggaccctcgt
      301 ctccggtcct gggcgcacct gaagaccaag tggcgtcgtg actcggcctt gcgcactcct
      361 tacgagcgcc ggcaagcgct ggtcgaattg gatgccttgg cggcactgag cttgggactg
      421 acggtcgaac agctgctctt gatttaccgc gtccagttcc cagtgctcca gcaatacgag
      481 cgcgagacct tttacgacca acgcggcaag atcgtgttca ccacgaaccg cgggctctcc
      541 ggcgtgggtc tggagcgtaa gcaatgggaa cagattcgcg acgccagcgc gggcgagaag
      601 ctgccgccgt gggcgaagga cgcagggggt gccttcgttc cgccttttga cagctgcgac
      661 cgggaggcgg acatggcgca ggcctaccac cacttcgcgg cccgactcgg gatcgggggt
      721 cagtcaaacg catgatcccg agcgtggtcg gctcagagat ccgtcgagcg ctgctcgact
      781 acctgaagac cacgtttcgg ctgcaggacg cggagctcga agcagccctg ttccggtacc
      841 tggagtccga tgagacgggc atgttccagg ggccctacgt ggacgtgcgg ctcccctttc
      901 gacaagccga cgaacgctgg gagtccagct ctcccctgga ttttggtccg cccttcgtgc
      961 cttacgcgca ccagctgcgc gcgttcgagc gcctgagcgc gcgcgacagg gtaccccaga
     1021 atacactcat caccaccggc accggctcgg gcaagaccga gtgtttcctg tatccggtcc
     1081 tcgatcactg ccgtcgcgcg cgtcagcgag gtgagctcgg catcaaggcc atcgtccttt
     1141 atccgatgaa cgctttggcg tccgaccagg ccgagcgcct cgccaagctg ctccgcgacc
     1201 cgctgctgca tggcgtctcc gcggggctgt acgtcggagg gaagggccaa cacgcggtac
     1261 gaggagcgtc ccacttggtg gatgatcgcg agatcttgcg caagtccccg ccggatatcc
     1321 tgctcacgaa ctaccggatg ttggatttct tgctgatgcg gccagaagac ggcgcgttat
     1381 gggcgctgaa cggtccgcac acgttgcggt acttggttct ggacgagctc cacacctacg
     1441 acggcgcgca gggcagcgac gtagcgtgcc tgatccggcg cttgaaggcc cgcctggaca
     1501 ttcccagcgg ccacctgtgt tgtgtaggca cctcggcgac catcggcagc ggcggcgctc
     1561 aagaccctcg ccagctgctc gccgatttcg cgacgcagat tttcgccgag cccttctctg
     1621 cggacagcct aatcggcgag gaacggctca caccggatga agccttcctg aagttcgagt
     1681 cgaagccggc gtttccgtat atcgaggatc gcccggactg gacgcgtctg gatgcgaccc
     1741 gctatgcccg ccacgaagcc tatctcctgg cgcaggtgga ggcgtggttc ggggaccctc
     1801 gaccgcgcgg gccgtcgctc ttggccgaga gcctcggcgc acatccgttc atggccaagc
     1861 tgctgcgggc cttggcgggt cgcgagcggc ggtcggggcc gcgccacgtg gctgaggtga
     1921 tgagcgcgat cgccagcgaa gagccgagct tcgctgagct cagccgggag cagcagtggc
     1981 tggtgctctc gtcttttgtg tccttgatcg cgcacgcgcg ttctacggac gcttcgggcg
     2041 aacgccgccc gtttctgcag gtgcaggtcc aactctggat tcgggaggtg catgggctct
     2101 tgcggcgcgt gggctccgga ccacccgcgt tcgcgtggca agacgatctc aagaccgagc
     2161 ccggcgagca ctggctcccg ttggtctttt gccgggagtg cggcttctcc gggtacgccg
     2221 ctgcgctgcg cgagggggaa accgcttttc gtgacgctcc cggagagatt ggcgaggtct
     2281 tccttcacaa cggcaaccgg ggccgcgtga ttgaggtctt gggcgagcaa cacggcgcgg
     2341 aacgtcgcgc gggagtcccc gatcaggcct gccaaaagca gctctgcccg cgttgcttga
     2401 gcctcggggc cgatgggaat tgccgctgcg atccgcgtgg catcccgact ttgccagtac
     2461 gcgtgcaccc ggagctcgat gaggacaaca agccaggccc gctgcggcgc tgcccggatt
     2521 gccaggcaga cgatgccctg agcttcctgg cctcgcgcgc cgcgacgctc tcgagcgtcg
     2581 ccgtatcgga gttgttctcg agccgcttca atgcggacaa gaagctgctt gccttcaccg
     2641 actcggtcca agatgcctcc caccgcgcgg gcttcttcgc ctcgcgcacg ttccgattcg
     2701 cgctgcgcac ggtcgttcaa gcgctcgtcg agcaccacgc cggtcagccg ctgccgctcg
     2761 cgagcttcgc cgctggattg atccgacact ggtcggagca actggacgaa ccccgagcgg
     2821 cggcgctgct gatgccgccc gatctggtcg aggatccg
//
LOCUS       DASTKU010000031        20243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_897414, whole genome shotgun sequence.
ACCESSION   DASTKU010000031 DASTKU010000000
VERSION     DASTKU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 20243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20243
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897414"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VFQ61_00965"
     CDS             <1..1410
                     /locus_tag="VFQ61_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953037.1"
                     /translation="QNPAFEIPDLDLPAPNPGRSQTGLTAVRPPEGPASGPMSGGANA
                     SGGANAHRSPANAPRDTPRARETGPRLAFDTQGDDDLASPQFDLELSAPISKGSAASV
                     FPYGGGLLSELPGFDPLHGIGPKLTVAPAAKVNYPSGVTPDRSRIVLDYNEIRALAKF
                     GEPPSMGPLTPLYAIRVTLARRALSSHLRQVERELMQAERKRDALLAELGRSKRAELE
                     SAPRFAAPLGDLEQLEGQGRQLAADASNEQAAHMAEVTRLRAELEQQKKQCERHQAAT
                     IELVNQGEALEQSKQRLDARYKRCFIEIRALPSHDPQRAALEQQALALHAELQAAAKN
                     QAANEEQKRLAVSAARRADDRVQELQRELNQRERQQEQKSSIVSQQLGNGSALQGAAL
                     ADLGRAVLSGRGEIPVDPATLQKLMRADQEVAEWVKQSELHLRAFDICDAQKVRAGWI
                     WLAVWLVLPVLYALKVALL"
     gene            1615..3315
                     /locus_tag="VFQ61_00970"
     CDS             1615..3315
                     /locus_tag="VFQ61_00970"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET9953038.1"
                     /translation="MSAPSSNDPKSILRDPSALNESRDSDLAVNPAGSPAQARISTEP
                     PRPAPVSSGQGKLEVLLARDAQALIGGGPTRLARQHAAGKLSARERLDALLDPGSFTE
                     IGRFVTNHAHEFGMDRQRYPGDGVVTGHGRVDGRLVFVYAQDFTVLGGSLGAAHAQKI
                     VRIMDQAMKVGAPVIGLCDSGGARIQEGVESLAGYADLFLRNTLASGVVPQISCILGP
                     CAGGAVYSPAITDFVCMVEGTSYMFITGPDVIRAVTHEEVTKEELGGASTHASKTGAC
                     HFVTEDDRACMALVRELLSFLPSNNAEDPPVARSDDPTDREIPELDRWVPDESNKAYD
                     MGALLRKVVDDEHFFEVHADYARNIVVGFARLGGRPIGVVANQPLYLAGVLDIAASIK
                     AARFVRFCDCFNLPIVTWVDVPGFLPGVDQEHRGIIVHGAKLLFAFAEATVPKLTVIT
                     RKAYGGAYDVMSSRHIRADVNLAFPTAEIAVMGPEAAVNIVYKNEIESAEDPASARAS
                     FIRDYRERFANPYEAAELGFVDEVIQPRQVRSRLIAHLSALTTKRDRNPPKKHGNIPL
                     "
     gene            complement(3368..4456)
                     /locus_tag="VFQ61_00975"
     CDS             complement(3368..4456)
                     /locus_tag="VFQ61_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HET9953039.1"
                     /translation="MNLLLEADDWKAQLRGSVRSVAELQRRLKLTPPELEGALRAEAG
                     GFPISITPYYLSLCDPEDPNCPVRRQVVPHAAEAREVPGDLRDPLGEEAHEAAPDLVQ
                     RYPDRALLLVSDRCAVYCRFCTRSRMVGAGGGPRSEERLSAAFDYLAAHPEVRDVIVS
                     GGDPLTLATERLCRVLERLRQIPSVETIRLATRVPVVLPQRITQELLQALRAYHPIWV
                     MTHFNHPKELTERSRAALVALADAGFPVMNQTVLLRGINDDSRVLSELFRGLVRVRAR
                     PYYLLQADPVRGTGHLRTSIATANRVFGELQGKLSGIALPKLIVDTPGGFGKVPIGPD
                     WVVSRSPGVTRFRTPRGIEVDYVDPPES"
     gene            complement(4453..5550)
                     /locus_tag="VFQ61_00980"
     CDS             complement(4453..5550)
                     /locus_tag="VFQ61_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9953040.1"
                     /translation="MSKSVVAILAQARISGLARRGSASLLGGLLLAGMVLGLPACRKG
                     GDKPGKAASSAAPSASARPVTLTPEQSAQVLARIGDRTITLGDYAAALDRMDVFERMR
                     YQSPERRRLLLNEMIELDLLAEEARRRGLDKTPETQERLRQMLRDELLRDVRRGVKSQ
                     NEFSEAEVRKYYDEHKADFQEPERRRVSHIQLASLAEAKRVLEKAKDVNAADWGKLVE
                     QYSTDRGDKSVGAMPRELAGDLGIVGPPGHPRGSNPRVPEALRAAVFKIDPKTPVLDE
                     VVQDGGKFHVVRMASRTEARERSYEEAQRSIRVTLIQEAVKQREADLEKELRAKYPVT
                     VDEAALAKLHAPKPSPSSKGVPGKLPTRPLP"
     gene            5662..6423
                     /gene="pyrH"
                     /locus_tag="VFQ61_00985"
     CDS             5662..6423
                     /gene="pyrH"
                     /locus_tag="VFQ61_00985"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET9953041.1"
                     /translation="MKTPRDGTTTGSVDSEANLKYRRVVIKLSGEALSGKEGGSGIDT
                     AMLKATASELAEVHATGVQIGIVVGGGNIFRGLRGASEGMDRAQSDNMGMLATVINSL
                     ALQDALERCGVHTRVMTALEIKQVAEPYIRRRAIRHLERGYIVVFAAGTGNPYFSTDT
                     AAALRSMEIRADGLLKATKVDGIYDRDPRRHAEAKMLERVSYDRFLADHLGVMDSTAV
                     ALCRENGLPIRVFKMTRGNIRRVCLGENVGTTVTQ"
     gene            6733..9084
                     /locus_tag="VFQ61_00990"
     CDS             6733..9084
                     /locus_tag="VFQ61_00990"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HET9953042.1"
                     /translation="MWSLPEFGTSVIYAVLVAAAYTFAVALAAGRGHPRLLTSARLGA
                     YATCALVLLGVLMLTYAFVSHDFRIRYVSHYSDRSMPTIYLITSLWGGQDGSLLWWSF
                     LLACYTAICLTWLKGRYRQLQPYVIATLMVIISFFAVLMLFAANPFEASIAGARPDGE
                     GLNPLLQNYWMIIHPPALYTGFVGCSIPFAFCVAALVTGRLDNEWIVAVRKWMLFAFL
                     FLSIGNGLGMAWAYEELGWGGFWAWDPVENAACLPWFTASAYVHSTMIQERRNILKVW
                     NVFLICFTFLLTIFGTFLTRAGIITSVHSFAQSDIGRYFLWFIGLAGAASLALGVWRA
                     KELRANAEIESVASREAMFVVNNWALLGGMTFILASTMYPKLSELWGENTTVGPSFFN
                     RWMGPIGLVIFLLMGLAPLFGWRKTSNEALKRALYAPVGAFIGMTLLHIVFGKSLGYP
                     AFVEKDPLFAGAIGSLFQKIASAAPVLTIGLAAFNVAVIVQEFYRGAAARMRTAGQRN
                     AKESVAVALYRLVDKNRRRYGGYIVHLGICGMFVGFVGTAWNIDQETSLLPNQTTRVG
                     RYEMTYLGSRMCPGNPRCSPEEQADLTKRMVFADLQLKRDGKDLGILSPAKFIFHKSP
                     EGPTTEVAMRRSLREDVYTIVGTVDPQSKRATFKFHINPFVTWIWLGISVLVVGAAIS
                     LWPDVSLGSSRAWAFARAGGSVAAGTMLSIWLAMTPGSAYARERPDKARPVLRVESPL
                     PRLVHSGAIWAPVLGLGLGLASAGSLSRRKRNSSLEAPTPPQS"
     gene            9081..9818
                     /locus_tag="VFQ61_00995"
     CDS             9081..9818
                     /locus_tag="VFQ61_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9953043.1"
                     /translation="MTRLNMSPRAFLAALCTVSLFAAIAAGMVYGIELSVLCLGAGAL
                     GLSLWSLWASVRSLSSDTPLTLDEAMALLAPSAAEEQKRSVLRALKDLEFERSVGKIT
                     EQDYREFSERYRAEARRLIENLDAAQAETRKLAEKLVADAVAKNAGTPSADSKERSTD
                     LEEEPGDPKKKSADPADAEHGAARDASADDTSVDTDADASELKDSAAVTCESCQTRND
                     VDARFCKHCGAPLGKPSVKLVSEKEAS"
     gene            9815..11320
                     /locus_tag="VFQ61_01000"
     CDS             9815..11320
                     /locus_tag="VFQ61_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953044.1"
                     /translation="MMRPFGLSRWIFAALTTWTAAALGQGNLPAHPPTATPGAVAAPA
                     ASVGASPPAAHGSTPTAKAAAGNANPHVAPPTSAAPGTGAAPGASATGTAAAPNGAAA
                     ARPAPANPHVGMNANGEVSRDESQPAGDLPKGTIEAWLLNEQDQPLANTEVRLAILFQ
                     RISEGESRTERRARTDESGRVTFSGLSTGSDFTYRVVAQSGPAQYGSPGFALADVGHR
                     VRLHVYPVTSDLNQVLVGMRGFVYVEPRDEVFQFDVLYRVFNVGNTTWVPNDVVMELP
                     EGFKAFKAQEGEGDAGFELVEGRGARLKGTFTPGQHDVNVRFQLPNQGDSSVRFRVGL
                     LPHVAELRVIAEASSQMNLEVGGFETPQVSSNRAGNRVLVTRRLLQRGEEGLKAAEIS
                     LTGIPVPSSGRWIAVLIASGLAVLGWLSASGRVRLEGIPEQKALEDHRRARDLLLEEC
                     VRVEKARARGQLGPKAYADARKTLLDALSRLGPEALADERRKRRRSSAQAA"
     gene            11795..12946
                     /gene="dnaN"
                     /locus_tag="VFQ61_01005"
     CDS             11795..12946
                     /gene="dnaN"
                     /locus_tag="VFQ61_01005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET9953045.1"
                     /translation="MQVVVSKKDLLRVLGRCQGVADRKSTMPALGNVLLEVKAPDRLR
                     IAATDLHLAVSGTINAEVQTPGSVAVGARDIFERVRMMPDGPLAISVGANAATTIRAV
                     GSARRFTLHGMPGSEFPSLPEPEPELPVLSLDARALSRLITATHFSISTDDTRLHLNS
                     ALFEWEGESVRMVTTDGHRLSKMDLLARGKQASASFLIPLKGVLELKRLCDESAQEAR
                     GEAAGEPYEVRIVQSGPNAFFELSGLRFSVKLVDAQFPPYQQVIPEQSEHSVRAPRLE
                     LADALKAVSVAANDRTGGIKLSLSESKMRFESESPESGQGFDEFPVDYVGSEITIGFN
                     ARYFLDVLNAVEDDEVMLGISGELDPVVIRPATASGDSTYLAVVMPMRI"
     gene            12947..14077
                     /gene="recF"
                     /locus_tag="VFQ61_01010"
     CDS             12947..14077
                     /gene="recF"
                     /locus_tag="VFQ61_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741333.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HET9953046.1"
                     /translation="MGDLPEPQRSRAGEIAFRRLSLTGFRNFERLVFEPCPRLNVIAG
                     NNGQGKTSLLEALYYVATTRSFRSERMAALVRHGASEAWVKAQISEGSHAREQRAGIV
                     RGARRITIDGKKPERLVAYAQRTPVVAFHPGDLELVSGPESLRRLLLDRVLLFIDAAS
                     YERKARYERAMKERKRALDERGERAPDLDAFETLAAQEGARLQTARGNAARELAVQLG
                     PLFADLSHRTLGLDVRYVPGGTEDVGAFQRELQQRRHIDRIRLSATFGPGRDQLELAI
                     DGRAARTHASQGQQRILTLALKLAELECIARARNLAPVLLLDDVSSELDPTRTGAVYS
                     VLSNSQSQIFVTTTRPELFPTPSAHASERRDFVIVSGEIHGA"
     gene            14270..16726
                     /gene="gyrB"
                     /locus_tag="VFQ61_01015"
     CDS             14270..16726
                     /gene="gyrB"
                     /locus_tag="VFQ61_01015"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET9953047.1"
                     /translation="MTTEAEEATPKASDYDSSNITVLEGLEAVRKRPGMYIGDVHDGS
                     ALHHLVWEVVDNAVDEHLAGHCNTVHISVHFDGSVTVDDDGRGIPVGMHERGVSAAEV
                     VMTVLHAGGKFDHSSYKVSAGLHGVGVSAVNAVSEWLRLEIHREGKVWFQEYRRGAPV
                     GPISAIGESDRTGTRITFKPDHEIFSNTEYSYDILANRLRELAFLNSGLVIHLKDERA
                     ERGETYEYKGGIREFVALLSKTREPVHEDVIAFTGEFPAGEGKMSIVVDLALQWTNSY
                     SEQILCYTNNVFNRDGGTHLTGLRSALTRTLNSYGQAQNLFKDAKNGLTGDDTREGVT
                     CVIHVKHPDASFDSQTKSKLVSSEVKGIVENAVSEHVTRYFEEHPQTARKILEKAVVA
                     AKAREAARKAREVVRKGVLDSTSLSGKLADCQSKDPSVSELYIVEGESAGGSAKQGRD
                     RHFQAILPLKGKILNVERARLDRMLSSQEVATLISALGCGIDENGQFDVSKLRYHKII
                     LMTDADVDGSHIRTLLLTFFYRQMREIIEKGYLYIAQPPLYGVKKGKRLMYLKDQPAL
                     DRFLTENGIEDMTVQAQKGPVLAGLPLYNLSTRLRSLRGILGKIDRRADARVVAALLR
                     TSPLSLADYRDRDKVEAAARTLETALIARYPDLCPLSVTVEWEKAHGAGRLVTRFRPG
                     ASTRPASLDWELAESAEYQELLSIWEDIRSIGAAPYEVRIGNAEPLSVPDPEALESLL
                     AERGRKGMSITRYKGLGEMNADQLWETTMSPDARTLLQVKINDPVRADELFSVLMGDQ
                     VEPRRQFIEENALNVRNLDI"
     gene            17123..17836
                     /locus_tag="VFQ61_01020"
     CDS             17123..17836
                     /locus_tag="VFQ61_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953048.1"
                     /translation="MKGISSKVLLALAFLASVPVACGDDDDSPMYVPAESGGRSSGGA
                     SSGGKGGRSSTDAGAGQGGQSVLQGGESGDTAERGGSAGGGQTSEPTGGTNEAGGTPA
                     TGGAGGATQVEGGAPSGGTTSEAGAGGGTGEVSLCDRACNVIMQHPTCQTGEFTMDDC
                     RRSVCVLQEQSTQCRYDLVLKCLGDQEGPGDWSICTSPTASYPVNYSKASGNPCYDEI
                     QAYSDRVLINASCDKYKDL"
     gene            complement(18008..19885)
                     /locus_tag="VFQ61_01025"
     CDS             complement(18008..19885)
                     /locus_tag="VFQ61_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9953049.1"
                     /translation="MRRHATAARLSTGTWLLLWGALVLGGVITNGCVDKFVACDAPGA
                     RPCPLLAGGQGGTTVTPIPTAGEGGSGGETSVPMPEGGMAGAPVDPRKPCKKEGLTTC
                     SGPAQSNYEICRGGFLEERECEAGSLCDSTDNKCKKLAEGCEERKPGERFCSGDVLHV
                     CGDDLVTVSSIQCDGACFEGDCRNPTCGDGKPAEGEACDDGNTTPGDGCSATCQWEPI
                     RVEAGHSTICALFKNGDLKCWGANQFGQLGIGNVDDMGDNPGEMGKDLPVAMTEVVDF
                     AVGRQHVCAAASSGGVYCWGDNSYGQLGESPDSGHLSSFETRPVPTRLPPAKVIALTA
                     GDWHTCALMKDQQVYCWGGNKQAQCGAGFGSDYYPQEADTPFAPAGLGGETPRAIGAG
                     FDLTCALLDTRVRCWGANSDGQSGVSSRGEAVPIANRDVSLGTFPVSSIAVGRAHVCA
                     RAETGDVKCWGLNGNGQLGHENVASVGEDQTQMGTLLSATRIGNALSIQAGGMSTCAM
                     LRKGEVVCWGSNDHGALGREYSVGNDNVGDDPGEMSSLVPVDVGANERIKSWSMGEKF
                     SCALLESGRIKCWGDNSDGQLGVGDTNDRGDSAGEMGDGLEYTWVGEDDESSLPKSAT
                     K"
BASE COUNT         3730 a         6464 c         6322 g         3727 t
ORIGIN      
        1 cagaatcctg ccttcgagat ccccgatctc gatctgcccg cgcccaatcc ggggcgttca
       61 cagacaggcc tgaccgcggt tcggccccct gaagggcccg catctggacc tatgtcaggc
      121 ggagcaaacg cgtcaggcgg agcaaatgcg caccgtagcc cggcgaacgc gccgcgagat
      181 accccaaggg cccgcgaaac cgggcccagg ctggctttcg atacccaagg ggacgacgat
      241 ctcgcgagcc cacagttcga tctggagctg tcggcgccga tctcgaaggg cagcgcagcc
      301 tcggtcttcc cgtacggggg cggcctgctg agtgaattac caggattcga tccgctgcat
      361 gggatcggcc ccaagctgac cgtcgcgccc gccgcgaagg tgaactatcc cagcggagtt
      421 acgccggacc gctcgcgaat cgtgctcgac tacaacgaga ttcgggcgct cgcaaagttc
      481 ggtgaaccgc ccagcatggg gccgctgacg ccgctttacg ccattcgggt aacgctcgcc
      541 cggcgcgcgc tgagttctca cctccgccaa gtggagcgtg aactcatgca agcggagagg
      601 aagcgcgacg ctttgctcgc cgagttgggt cgatcgaaac gagcggagct cgagtccgca
      661 ccccgcttcg ctgcgccgct cggggatctc gagcaattgg agggtcaagg ccggcaactg
      721 gccgccgacg cgtccaatga gcaggccgcg cacatggccg aggtcacccg cttgcgcgcg
      781 gagctcgaac agcagaagaa gcaatgcgag cgtcaccaag ccgcgacgat cgagctcgtc
      841 aaccagggtg aggcgctgga acagagcaag cagcgcttgg atgcacgcta caagcgctgc
      901 ttcatcgaaa ttcgagctct gccctcgcat gatcctcaac gcgccgcgct ggaacagcag
      961 gcgctcgcgt tacacgcgga gttgcaagcg gcggcgaaaa accaagccgc caacgaggag
     1021 cagaagcgct tggcggtgag cgccgcccgc cgcgccgacg atcgcgtgca agagctgcaa
     1081 cgcgagctga accagcgcga acgtcagcaa gagcagaaga gcagtatcgt gagccagcag
     1141 ctcggtaatg gctcggcgtt gcagggcgcc gcgctcgcgg atctagggcg cgcagtattg
     1201 tcaggccgcg gtgaaattcc tgtcgatcct gccacgttgc agaagctgat gcgagcggac
     1261 caagaggtcg cggagtgggt aaagcagtcg gagctgcacc tgagagcctt cgacatctgt
     1321 gatgctcaga aggtaagagc cggctggatt tggctcgccg tctggctggt gttgccggtg
     1381 ctctacgcgc tcaaggttgc actcctatag ggttttgccg cgctccgtta gcagctcgct
     1441 cgctcacgaa ccggttcccg tcgcgtcgtt gctcgcggcc ctagcgctta cggtccccct
     1501 gccgtgcctc gtccggcgtt gtgctgattg aaacgtattc gttgagccgt tttcaatcgg
     1561 atatgccgtt ttggagccgt ggttagttgg tgccaatgat agggtcgagc acgtatgtcg
     1621 gcacccagct cgaacgatcc gaaatcgatt ctccgggatc ccagcgctct caacgaatcg
     1681 cgcgactccg atctcgccgt taatcctgcg ggcagcccag cgcaagcgcg aatctcgacc
     1741 gaacccccgc gcccggcgcc cgtcagctcg ggtcagggaa agctcgaagt gttgctggct
     1801 cgcgatgcac aagcactgat cggaggcggc cccacgcggc tcgcacgtca gcacgccgcc
     1861 ggtaaactta gcgcgcgtga acgcctggat gcgctgctcg atccgggttc tttcacggaa
     1921 atcggtcgct tcgtaacgaa ccacgcccac gagttcggga tggatcgaca acgctatccc
     1981 ggagacggcg tcgtgaccgg acatggccgt gtagatggcc gcctcgtatt cgtctatgcg
     2041 caagatttca ccgtgctcgg cggttctctc ggcgccgccc atgcccagaa gatcgtgcgc
     2101 atcatggatc aagccatgaa agtaggcgcc cccgtgatcg ggttgtgtga ctccggtggt
     2161 gctcgaatcc aggagggtgt cgagagcttg gccgggtatg ccgatttgtt cttgcgcaat
     2221 acactcgcca gcggcgtcgt gccgcaaatc agctgcatcc tgggcccctg cgcgggcggg
     2281 gcagtctatt cgcccgccat caccgacttc gtatgcatgg tggagggtac gagttacatg
     2341 ttcatcacgg ggccggacgt gatccgcgcc gtgacgcacg aggaggtcac gaaggaggag
     2401 ctcggcggcg ccagcacgca cgcgagcaag accggtgcgt gtcacttcgt gaccgaggac
     2461 gatcgcgcgt gcatggcctt ggtgcgcgag ttgctctcgt tcttgccctc caataacgcg
     2521 gaggatccgc cggtcgcccg cagcgatgac ccaacggatc gtgaaatccc ggagctcgat
     2581 cgttgggtcc cggacgaaag caacaaggcc tacgacatgg gcgcgttgct cagaaaagtg
     2641 gtggatgacg aacatttctt cgaggtgcat gccgactacg cgcgcaacat cgtggtcggc
     2701 ttcgcgcggc tcggcgggcg gccgatcggc gtcgtcgcga atcagcccct ctacctcgcg
     2761 ggtgtgctcg atatcgcggc gagcataaag gccgcccgct tcgtgcgctt ctgcgactgc
     2821 ttcaaccttc ccatcgtgac ctgggtggac gttcccggat ttttacccgg agtcgatcaa
     2881 gagcaccgcg gcatcatcgt gcacggggcg aaactgctgt tcgctttcgc cgaggctacg
     2941 gtgccgaagc tcaccgtcat cacacgcaag gcttacggtg gagcttacga cgtcatgagc
     3001 agccgtcaca tccgcgcgga cgtcaacttg gccttcccaa ccgccgagat cgccgtgatg
     3061 gggccggaag ccgcggtgaa tatcgtttac aaaaacgaga tcgagtccgc agaggatccg
     3121 gcgtcggcgc gcgcgagctt catccgtgat taccgcgagc gctttgccaa tccgtacgaa
     3181 gcggcggagc taggattcgt cgacgaggtc atccagccac ggcaagtccg gtcccgactg
     3241 atcgctcact tatccgcgtt gaccacgaag agagatcgca acccgcccaa gaagcacggc
     3301 aacatcccct tgtgagcccc gcgtcgagga atgtgatgcc attcgccgac gaacacgcag
     3361 cttccgatca actctcgggc ggatcgacgt agtctacttc gatcccacgc ggagttctga
     3421 agcgagtcac gcccggcgaa cgcgagacga cccaatccgg tccgatcggg accttgccga
     3481 agccgccggg agtatcgacg attagcttcg gaagcgcgat cccagagagc ttgccttgca
     3541 gctctccgaa tacccgattg gccgtcgcaa tcgaggtccg caagtgaccc gtgccccgca
     3601 ccggatcggc ctgcagaaga tagtaaggac gtgcccgcac ccgcaccaga ccacggaaca
     3661 gctcgctcag cacgcggcta tcgtcgttga tcccgcgtaa cagcacggtt tgattcatca
     3721 ccgggaaccc cgcgtccgcc aacgccacca gcgccgcccg cgagcgttcg gtcaactcct
     3781 tgggatgatt gaagtgggtc atcacccaaa tcgggtgata cgctcgcaac gcttgcagca
     3841 actcctgcgt gatgcgctga ggcagaacca ccggcactcg agtggccaaa cgaatcgtct
     3901 cgacgctcgg gatttggcgt aaccgctcga gcacccggca cagccgctcc gtcgcgagcg
     3961 tcaacggatc gccgccgctc acgattacgt cgcgcacctc gggatgcgcc gccaagtaat
     4021 cgaaagccgc gctcaagcgc tcctccgatc gtggcccgcc ccccgcgccg accatgcggc
     4081 tacgcgtgca gaatcggcaa taaaccgcgc atcggtcgct gaccaagagc agggctcggt
     4141 caggatagcg ttgcacgaga tctggcgcgg cctcgtgggc ctcctccccg aggggatccc
     4201 gcaagtcccc cggaacttcg cgcgcctcgg ccgcgtgcgg caccacctgg cgccgcaccg
     4261 ggcaatttgg atcctccgga tcgcacaagg acaggtaata aggcgtaatc gagatcggga
     4321 agccgccggc ctccgcgcga agcgcgccct cgagctcggg cggggtcagc ttcagccgac
     4381 gctgcagctc ggcgacgctc cgcaccgaac ctcgcagctg cgctttccaa tcgtccgcct
     4441 cgagcaatag attcaaggca aaggcctcgt cggcaactta cccggcaccc ctttactgga
     4501 tggcgagggt ttgggcgcgt gcaacttggc cagcgccgct tcgtcgacgg ttacggggta
     4561 cttggcgcgt agctcttttt cgaggtcggc ctcgcgctgc ttcaccgcct cttggatcaa
     4621 cgtgacccgg atcgaacgct gcgcctcttc gtaagagcgc tctcgcgcct ccgttcgtga
     4681 agccatgcgc acgacgtgga acttgccacc gtcctgcacg acctcatcca gaactggagt
     4741 tttcggatcg attttgaaga ccgcggccct caaggcttcg ggaactcgag ggttgctgcc
     4801 acgcggatgg cctggcggcc ctacgatccc caaatccccg gcgagctcac ggggcatcgc
     4861 gccaacggat ttatctccgc gatcggtaga atactgctcg acgagcttac cccagtcggc
     4921 cgcgttgaca tccttagcct tctccagcac gcgcttggct tccgccaagc tcgcgagctg
     4981 aatgtgcgag acgcggcgcc gctccggctc ttggaagtcc gccttgtgct cgtcgtaata
     5041 cttgcgcacc tcggcctcgc tgaactcgtt ttgagacttc acaccacgcc gcacatcacg
     5101 cagcagctcg tctcgcagca tttggcggag ccgctcctgg gtctccggtg tcttgtccaa
     5161 gccccgccgg cgcgcctctt cagccagcag atcgagctcg atcatctcgt tcaaaagcag
     5221 tcggcgccgt tcgggggact ggtaacgcat gcgctcgaac acatccatgc ggtcgagcgc
     5281 cgcggcgtaa tcccccagcg tgatcgtgcg gtcgccgatg cgagccaaga cctgcgcgga
     5341 ctgctccggg gtcaacgtga ccgggcgcgc gctggcgctg ggcgccgcgg agctagccgc
     5401 tttccccggc ttatcgccgc ccttgcggca ggccggtagc ccgagcacca ttccggccag
     5461 gagcagaccg ccgagcagcg atgcggagcc ccgccgagct agcccggaaa tgcgtgcttg
     5521 cgccaagatc gcgacgaccg acttcgacat gcgtggctcg attactacgc ccgctgccgt
     5581 gcgcaaatcg cgattgcaaa aaggcgggcg ggcgccgcag agatagcccc gaacaaaaga
     5641 aaaaccagcg gaaactgccg gttgaaaacg ccccgcgacg ggactacgac cggtagcgtg
     5701 gactccgaag ccaacctcaa gtaccggcgc gtcgtcatca aactcagcgg tgaagcgtta
     5761 agcggaaagg aaggcgggtc gggcatcgat accgccatgc tcaaggctac ggcgagcgag
     5821 ctcgccgaag tgcacgcgac cggcgttcag atcggtatcg tggtcggcgg gggcaacatc
     5881 ttccgtggct tgcgcggcgc gagtgagggc atggatcgtg ctcagagcga caacatgggt
     5941 atgctcgcca cggtcatcaa ctcgctggct ctgcaagatg ccctcgagcg ctgcggagtt
     6001 catacgcgtg tgatgacggc gctcgagatc aagcaagtgg cggagccgta cattcggcgc
     6061 cgcgccattc gccacctgga gcgcggctac atcgtggtct tcgcggcggg taccggcaat
     6121 ccgtactttt cgaccgacac tgcggcggcc ctgcgctcga tggaaatccg cgccgacggt
     6181 ttgctcaaag ccaccaaggt ggacggcatc tacgatcgcg acccgcgccg ccatgcggag
     6241 gcgaagatgc tggagcgtgt gtcgtatgac cgcttcctgg ccgatcacct gggcgtgatg
     6301 gattccaccg ccgtagcttt gtgtcgtgag aacggcttgc cgatccgcgt tttcaagatg
     6361 acccgcggca acatccgccg ggtgtgcctt ggcgaaaacg tcggcaccac tgttacgcaa
     6421 taggtcgaag cgtgagcgcg ccgtggcccg cggggccacg gcaaaccacg aacgcttcga
     6481 agcctgagga gcgcgcgtct acgtcgtttt gggcgctctt tccgcgcaaa agcgcggccg
     6541 acgtagatgg gttgcaaacg cgagcactaa cgggtggccg ctggcaggac accagaggct
     6601 ggtccggcag cagactgggc agctgcgaac ccgaatcgga agtgcacctc gagctcgagc
     6661 cggcctagcc agaatggctc aactcgagtg ttttctgccc gagcgtgaaa tccgggctaa
     6721 gctccgccga ccatgtggtc gctccctgaa ttcggcacga gtgtgatcta tgcggtgctg
     6781 gtagccgcgg cttacacgtt tgcagtggcg ttagccgcgg gacgtgggca tccgaggtta
     6841 ctgacttcgg cacgactcgg agcgtatgcc acgtgcgcgt tggtgctgct gggcgtgctg
     6901 atgctcactt acgccttcgt ctcgcacgac ttcagaatcc ggtacgtctc gcattacagc
     6961 gaccgctcca tgccgacgat ttacctgatt acgtcgctat ggggagggca agatggctca
     7021 ctgctctggt ggtcattctt gctcgcttgc tacacggcga tctgcctgac ctggctcaag
     7081 gggcgctatc ggcagctaca gccttacgtc atcgcgacgc tcatggtgat catctcgttc
     7141 ttcgccgtgt tgatgctgtt cgccgcgaat cccttcgagg cgagcatcgc gggggcgagg
     7201 ccggatgggg aggggctgaa tccgctgctc cagaactact ggatgatcat ccacccgccc
     7261 gcgctgtaca cgggcttcgt cggttgctcg atcccgttcg cgttctgtgt tgcagcgctg
     7321 gttacggggc gcctcgacaa cgagtggatc gtggcggtcc gcaaatggat gctgttcgcg
     7381 tttttgttct tgtcgatcgg caacggcttg ggcatggcct gggcctacga ggaactcggc
     7441 tggggtggct tttgggcctg ggatccagtc gagaacgccg cctgtttgcc gtggttcacc
     7501 gcgagcgcct acgtacattc cacgatgatc caggagcgtc gcaacatcct caaggtctgg
     7561 aacgtgttcc tgatttgctt cacgttcttg ctcacgatct tcggcacgtt cctgacccgc
     7621 gccggcatca tcaccagcgt acatagcttc gcccagagcg atatcggtcg ctacttcctg
     7681 tggttcatcg ggctcgccgg cgccgccagc ctcgcgctcg gggtgtggcg agcgaaggag
     7741 ctccgagcca acgcggagat cgagagtgtg gcgagccgcg aagccatgtt cgtcgtgaac
     7801 aactgggcct tgctgggtgg catgaccttc attttggcga gcacgatgta tcccaagctg
     7861 agcgagctct ggggtgagaa cacgacggta ggcccctcct tcttcaatcg ctggatgggc
     7921 ccgatcggac tcgtgatctt cctgctcatg ggcctggccc cgctcttcgg ctggcgcaaa
     7981 acgagcaacg aggcactcaa gcgcgcgctc tacgcgccgg tgggcgcctt catcggaatg
     8041 acgttgctac acatcgtttt cggcaagtca ctgggttatc ccgcgttcgt cgagaaggat
     8101 ccgctgttcg cgggcgcgat cggcagtctg tttcaaaaga tcgcgtcggc cgcccccgtg
     8161 ctcacgatcg ggctcgccgc cttcaacgtc gccgtgatcg ttcaagagtt ctaccgcggc
     8221 gccgctgccc gcatgcgtac cgcggggcag cgcaacgcca aggaaagcgt agcggtggcg
     8281 ctctaccgct tggtggacaa gaaccgccgg cgctacggcg gttatatcgt tcatctcggg
     8341 atttgcggca tgttcgttgg gttcgtcggc acggcctgga acatcgacca ggagacgtcc
     8401 ctgttgccca atcaaactac gcgcgttggg cgctacgaga tgacctactt gggttcgcgc
     8461 atgtgccctg gaaacccgcg gtgtagcccc gaggagcaag ccgatttgac gaagcgcatg
     8521 gtgttcgccg atctgcagct caagcgcgac ggcaaagacc tcggcattct ttcaccagcc
     8581 aagttcatct ttcacaagag ccccgaaggc ccgaccaccg aggtcgcgat gcgccgctcg
     8641 ctgcgcgagg acgtgtatac gatcgtcggg accgtggacc cgcaatccaa acgggcgacc
     8701 ttcaagtttc atatcaaccc cttcgttacg tggatttggc tcggaatctc ggtgctggtc
     8761 gtgggcgccg cgatctcact gtggcccgac gtgtctctgg gtagctcccg agcatgggcc
     8821 tttgcgcggg ctggcgggag cgtagcggcg ggtaccatgc tctcgatttg gctcgcgatg
     8881 actccgggct cggcctacgc tcgggagcgt cctgacaagg ctcgacccgt tttgcgagta
     8941 gaatcaccgc taccacgctt ggtgcattcc ggcgctattt gggcgccggt gctggggctc
     9001 gggctcgggc tcgcgagcgc cggttcgctc tcgcgacgta agcgcaactc gtccctcgaa
     9061 gctccgacgc ctccgcaatc atgacccgct tgaatatgag cccccgcgcg tttctcgcgg
     9121 cgctttgcac ggttagcctg ttcgccgcga ttgcggccgg gatggtgtac ggcatcgaac
     9181 tctcggtgct gtgcctcgga gcgggcgccc tcggcctctc gctctggtcg ctatgggcga
     9241 gcgtgcggag cctctcatcc gacacgccac tcacactcga cgaagccatg gcgttgcttg
     9301 ccccgagcgc cgccgaagag cagaagcgct cggtgctgcg agctttgaag gatctggaat
     9361 tcgagcgcag cgtgggcaag atcaccgaac aagattacag agagttctcc gagcgttacc
     9421 gggcggaggc gcggcgactg atcgaaaacc tggacgccgc ccaggccgag acccgcaagc
     9481 tcgccgaaaa actggtcgcg gacgcggttg cgaagaacgc gggcactccc tccgccgatt
     9541 cgaaagaacg gtccaccgat ctggaagaag agcccggcga tcccaagaag aaatcggccg
     9601 atcccgcaga tgccgaacac ggtgcggcac gggacgcctc cgccgacgac acctcggtcg
     9661 acacagacgc cgacgccagc gagctgaagg actcagcggc ggtcacgtgc gagagctgcc
     9721 aaacccgcaa tgacgtcgat gcgcgcttct gcaaacactg cggagcgccg ctcggcaagc
     9781 cctccgtcaa gctcgtctcc gaaaaggagg catcgtgatg cgtcctttcg gattgagtcg
     9841 ctggatcttt gccgcgctca ctacctggac tgcggccgcg ctaggccagg gcaacctccc
     9901 tgcacaccca cccactgcga cacccggggc ggtagcggca cccgccgcat cggttggcgc
     9961 tagccccccg gccgcgcacg gttccacccc gaccgccaag gctgccgccg ggaacgcaaa
    10021 tcctcatgtg gctccgccca ctagcgcggc gcctggaact ggcgcagctc caggtgctag
    10081 cgcgaccggc actgccgccg ctccgaacgg cgccgctgcc gcacggcccg cacccgccaa
    10141 tcctcacgtc ggaatgaacg ccaacggaga ggtctctcgc gatgagagcc agccggcggg
    10201 agatctaccg aaaggaacga tcgaggcttg gttgctcaac gaacaagacc agccgctcgc
    10261 gaacaccgaa gtgcgcctcg ccatcttgtt tcaacgcatc tcggaaggtg agtcgcgcac
    10321 cgagcggcgc gcccgcacgg acgaaagcgg tcgagtcacc ttcagtggtc tctcgacggg
    10381 aagcgacttc acttatcgcg tcgtggccca gagtggtcct gctcaatacg gatccccggg
    10441 attcgcgctg gccgacgtgg ggcaccgggt tcgactccac gtctatcccg tcaccagtga
    10501 cctgaatcag gtgttggttg gaatgcgcgg cttcgtttac gtggagccgc gtgacgaggt
    10561 gttccagttc gacgtgctct atcgcgtctt caacgtcggc aacacgacgt gggtgccgaa
    10621 tgacgtcgtg atggaactgc ccgagggctt caaagcattc aaagcccaag agggtgaggg
    10681 cgacgccggt ttcgagctcg tagaaggccg tggagcgcgc ctgaagggca ctttcacacc
    10741 ggggcagcac gacgtgaacg tgcgcttcca gctgcccaat caaggcgatt catcggttcg
    10801 tttccgagtc gggttactgc cgcatgtcgc cgagctgcgg gtgatcgcgg aagcttcgtc
    10861 ccagatgaac ctcgaggtgg gcggcttcga aacaccacaa gtatcttcga atcgggccgg
    10921 caatcgcgtg ctcgtcaccc gacgcctctt gcagcggggt gaagagggcc tgaaagccgc
    10981 cgagatctcg ctgaccggca tcccggtacc gagttccggt cgttggatcg ccgtcctcat
    11041 cgcgagcggg ctcgccgtac tggggtggct ctcggcttct ggacgtgtcc gcctggaggg
    11101 cattccggag cagaaagccc ttgaggacca ccggcgcgct cgagatctcc tgctcgagga
    11161 atgcgtgcgg gtcgagaaag cccgcgcgcg cggccagctc ggtccgaagg cgtacgctga
    11221 cgcgcgtaag actctgctgg atgcgctttc gcggcttggt cccgaggcac tcgctgatga
    11281 gcgtcgcaaa cggcgccgca gctccgcaca agccgcctaa gcagggaact tcagcgggcg
    11341 cccgcgggct tcgcctcgct gggcggtctt ttgtcggcta tttggcactt ctggccctga
    11401 cccgagtgct ggccgaacag cccaaactcg ctatccacag actgtggata accttgggat
    11461 atgcctatgg ttatcgcgtg tgacgcattg gggacgcctg ggtatccaga gagttaccaa
    11521 ctggtgaccc aggtcgtttc tgcacccaca tccacagcgt ggggaggaaa tagcccagcc
    11581 aatccagtga cttgcaaagc ttcccccagt ctccacagcc cctataacga ccaccatttc
    11641 tttaaattta cttagatcag gagagaagaa gagaccccct ccgtcccccg agctcgtgtc
    11701 ggccatgagg tcgaagtgac ctctcgcttt ccgcgctgga ggtgaccgcg aatggctggc
    11761 gtcacagacc ggacccggtt aggacgtcgc tccaatgcaa gtcgtcgtca gcaagaagga
    11821 cctgcttcgc gtcctcggtc ggtgccaggg agtcgccgat cgcaagagca ccatgcctgc
    11881 cctcggtaac gttctgctcg aagtcaaagc tcccgatcgt ttgcgtatcg cagcgaccga
    11941 tttgcatctc gccgtgagcg gaaccatcaa cgccgaggtg caaacgccag gctccgtcgc
    12001 cgtcggcgcc cgcgacatct tcgagcgcgt gcgcatgatg ccggatggtc cgctcgcgat
    12061 ttcggtcggc gcgaacgccg caaccacgat ccgagccgtc ggatccgcgc gacgcttcac
    12121 gttgcacggc atgcccggtt ccgagtttcc gtcgttgccc gagcccgagc ccgagctgcc
    12181 cgtgctgtca ctcgatgcgc gcgccctgtc gcgcttgatc accgccacgc acttctcgat
    12241 ctcgaccgac gatacccgcc tgcacctgaa cagcgcgctg ttcgagtggg agggtgagag
    12301 cgtgcgcatg gtgacgaccg atgggcaccg cttgtcgaag atggatctac tcgcgcgagg
    12361 caagcaggct tcggcctcgt tcctgatccc gctcaagggt gtcttggaat tgaagcgact
    12421 gtgcgacgag agcgcgcagg aagcgcgcgg cgaagccgcg ggtgaaccgt acgaggttcg
    12481 aatcgtccag agcggcccca acgccttctt cgagctgtcg ggactgcgct tcagcgtgaa
    12541 gctcgtcgac gctcagttcc cgccgtacca gcaggtgatc ccagagcaga gcgagcacag
    12601 cgtccgcgct ccgcggctcg agctcgcaga tgctctgaag gccgtgtccg tggccgccaa
    12661 cgaccgcacg ggtggcatca agctcagctt gtcggagagc aagatgcgct tcgaaagcga
    12721 gagccccgag agcgggcagg gtttcgatga attcccggtc gactatgtcg gcagtgagat
    12781 cacgatcggc ttcaacgcgc gctactttct cgatgtgctg aacgccgtag aggacgacga
    12841 agtgatgctc ggcatcagcg gcgagctcga tccggtagtg attcggcccg ccacggccag
    12901 cggtgattcc acgtatctcg cggtcgtgat gccgatgcgg atttgagtgg gcgatctccc
    12961 ggagccgcag cgttcccgag ccggcgagat tgctttccgg cgcctctcgc tcacgggctt
    13021 tcgcaacttc gagcgtctgg tgttcgagcc ttgcccgcgt ctgaacgtga tcgcgggcaa
    13081 caacgggcag ggcaagacga gtctgctcga agcgctctac tacgtagcca ccacacgtag
    13141 tttccgcagt gagcgcatgg cggcgctggt tcgtcatggc gcttccgaag cctgggtcaa
    13201 ggcgcaaatc agcgaggggt cgcatgctcg agagcagcgg gcggggattg tgcgcggcgc
    13261 gcgccgtatc acgatcgacg gcaagaagcc ggagcgtctc gtggcctatg cccagcgcac
    13321 gcccgtggtc gcctttcatc ctggcgatct cgagctcgtc tcgggacccg agagcttgcg
    13381 ccgcttgctg ttggatcgcg tgttgttgtt catcgacgcg gccagctacg aacgcaaagc
    13441 gcgctacgaa cgagcgatga aagagcgcaa gcgcgcgctc gacgagcggg gcgaacgcgc
    13501 acccgatcta gacgccttcg agaccctggc cgcgcaggag ggagcgcgct tgcagaccgc
    13561 tcgcgggaac gcggcacgcg agcttgcggt ccaactgggg ccgttgttcg cggacctctc
    13621 ccaccgcacg ttgggcttgg acgtacgcta cgtacctggc ggtaccgagg acgtcggcgc
    13681 gttccagcgt gagctccagc agcgacgtca catcgatcgc atccggctat cggccacgtt
    13741 cggtccgggt agagaccaac tcgaactcgc catcgacgga cgcgccgcgc gcacccacgc
    13801 ctcccaaggg caacagcgca tccttacttt ggcactcaag ctggccgagc tcgagtgcat
    13861 cgccagagcc aggaatctgg ctccggtctt gctgctcgac gacgtctcga gtgagctcga
    13921 tcccacgcgg acgggcgccg tttactcggt gctcagtaac agtcagagtc agattttcgt
    13981 aacgacgacg cgcccggaac tgttcccaac accgagcgct cacgcctcag aacgtcgaga
    14041 tttcgtgatc gtctctggcg agatccacgg ggcttagacc agggccttcg gagggcgccg
    14101 atcgagagct ggatcgcttg gaaaatccag ccaaattgct tgccgaaacc gtcaaaagca
    14161 agtatacaaa acgggctttt cgtgcggtgt gccgagccgt cagcggtcga gcagccgcgg
    14221 gtgaaaatag ctcgtcgcag atgcgaaagc gctgaagagg actcgacgtt tgacgacaga
    14281 ggctgaagag gcgacgccca aagcgtcgga ctacgactcc agtaacatca ccgttctcga
    14341 aggccttgag gccgtccgaa agcgtcccgg gatgtacatc ggggacgttc acgatggttc
    14401 ggccttgcac cacttggtct gggaggtggt ggacaacgcc gtggacgagc acctggctgg
    14461 ccactgcaac accgtccaca tctcggtcca cttcgacggc tccgtgaccg tggatgacga
    14521 cgggcgcggc atccccgtgg gtatgcacga acgcggcgtg agcgccgcgg aagtcgtgat
    14581 gaccgtgctg cacgccgggg gaaagttcga tcattcgagc tacaaagtca gcgcgggcct
    14641 gcatggggtc ggcgtgagcg ccgtcaacgc cgtctcggag tggctgcggc tcgaaatcca
    14701 tcgcgagggc aaggtctggt tccaagagta tcggcgcgga gctcccgttg gccccatctc
    14761 tgccatcggc gagagtgatc ggaccggcac gcgaatcacc ttcaagcccg atcacgagat
    14821 cttcagtaac accgagtaca gttacgacat cctcgctaac cgtctgcgcg agctcgcgtt
    14881 cttgaactcc ggcttggtga tccatctcaa ggatgaacgc gccgagcggg gtgagaccta
    14941 cgagtacaag ggcggcattc gggagttcgt cgcgttgctg agcaagacgc gcgagcccgt
    15001 gcacgaagac gtgatcgcgt tcaccggcga atttccagcc ggcgaaggca agatgtcgat
    15061 cgtggtcgat ctggctctgc aatggaccaa tagctactcc gagcagatcc tctgctacac
    15121 gaacaacgtc ttcaatcggg acggcggtac gcacctcacg ggtttacgct cggcgctgac
    15181 gcgcacgctg aattcgtatg gtcaggccca gaacctcttc aaagacgcaa agaatgggct
    15241 caccggggac gatacgcgcg aaggcgtgac ctgcgtcatc cacgtcaaac acccggatgc
    15301 ttccttcgac tcgcagacca agtccaagct ggtctcgagc gaggtgaagg gcatcgtcga
    15361 aaacgcggtt agtgagcacg tcacgcgcta cttcgaagag catccgcaaa cggctcgcaa
    15421 aatcctcgag aaggccgtgg ttgcggccaa agcacgcgaa gcggcgcgca aggcccgtga
    15481 ggtcgtgcgc aaaggggtgc tggatagtac ctcgctctcg ggtaagctcg cggactgtca
    15541 gagcaaggat ccctcggtca gcgagctcta catcgtggag ggtgagagcg ccggcggttc
    15601 tgcgaagcag ggtcgcgacc ggcattttca ggcgattttg cccctgaaag gcaaaatctt
    15661 gaacgtggag cgcgcgcgcc tggatcgaat gctctcgtcg caagaggtag ccacgctgat
    15721 ctctgccctc ggttgcggga tcgacgagaa cggtcaattc gacgtctcga aactccggta
    15781 tcacaagatc attctgatga cggacgcgga cgtggatggc agtcacatcc gcacgctgct
    15841 gttgaccttc ttctaccgtc agatgcggga gatcatcgag aagggttatt tgtacatcgc
    15901 ccagccaccg ctctatggcg tgaagaaggg caagcggctg atgtacttga aggatcagcc
    15961 ggcgctcgac cgcttcctga ccgagaacgg catcgaagac atgaccgtgc aggcccaaaa
    16021 gggcccggtg ttggcagggc ttccgctgta caacctctcg acacggcttc gctcgttgcg
    16081 cggaatccta ggcaagatcg atcgccgtgc ggacgcgcgc gtcgtcgcgg ctctgctccg
    16141 aactagcccg ctttcgctgg cggactaccg ggatcgggac aaggtcgaag cggcagctcg
    16201 tacgctcgaa accgcgctga tcgcccgtta tccggatctt tgcccactca gcgtaacggt
    16261 ggagtgggag aaggcacacg gcgccgggcg cctggttacc cgctttcgtc cgggcgcctc
    16321 gacacggccc gcgtcactcg attgggagct tgcagaatcg gccgagtacc aagagctgct
    16381 gtcgatttgg gaggatatcc gatcgatcgg agccgcaccc tacgaggtcc gcattggcaa
    16441 cgccgagccg ctgagcgtac ccgatcccga ggcgctcgag agtttgctcg cggagcgcgg
    16501 gcgcaagggt atgtcgatca cccgctacaa aggcctcggc gaaatgaacg ccgatcagct
    16561 gtgggagacc acgatgagtc cggacgcccg cacgttgttg caggtcaaga tcaacgatcc
    16621 ggtgcgtgca gacgagctat tcagtgtttt gatgggcgac caagtcgaac cgcgccgtca
    16681 attcatcgaa gaaaacgcac tgaacgttcg aaacctagac atctagggtc actccgactt
    16741 cgggcctgcc cctctacttt cgcagagatg aatctgcttt tgcagaggtg aacatctctc
    16801 cgggaagagg gtgcaggcca agggataggc ccgattccgt ctagtggcag ctcgcttgtt
    16861 tccgaattgc ctcttgcacc tgacggtttc agacagcggc atatagctgt tttcttgccg
    16921 atacgctgga gtttgctcac gaagcgtttc gctcgttcgc gtagtcgcac ctgctacacg
    16981 ggcgcaccga aagtggagat ggacggcctc cgctgtcggg aaccgattgc agatctgaaa
    17041 ctcgcgtgag atggtttgcc acccgcgcgg gcacctcgag gtttgagggt ccgtacggtg
    17101 tagcgctgac cagagagccg aaatgaaggg gatttccagc aaggtcctgc ttgcgctggc
    17161 gttcttggcc agtgtgcccg tcgcctgcgg tgatgacgat gactcaccga tgtacgtccc
    17221 ggcggagtcc ggcgggcgat cgagcggtgg cgcgagctcc ggtggaaaag ggggaaggtc
    17281 ctcgacggac gccggcgccg gtcaaggcgg gcaatccgtc cttcagggtg gggagtcggg
    17341 cgataccgcc gaacgcgggg gatcggccgg cggagggcaa acctccgagc ccacgggagg
    17401 taccaacgaa gccggcggca cgcctgcgac ggggggcgct ggcggcgcca ctcaagtcga
    17461 aggcggcgcg ccctccggcg gcactacgtc ggaggcgggc gctggcggcg gcacgggcga
    17521 agtatcgctt tgcgaccggg cctgcaacgt catcatgcag catccaacgt gccagaccgg
    17581 cgagttcacg atggatgact gccgccgctc cgtctgcgtg ctgcaggagc agtcgactca
    17641 gtgtcgctac gacttggtgc tgaagtgctt gggcgaccag gaggggccgg gtgattggag
    17701 catttgcacc agtcccaccg cttcgtaccc ggtgaactac agcaaggctt cgggcaatcc
    17761 ctgttacgac gagattcaag cctacagtga tcgcgtcctg attaatgcga gctgcgacaa
    17821 gtacaaagac ttgtgaggcg gcggcgatcg taacctgaag cccgatttcg ccgaaaagtg
    17881 tcgggcctgc cgccgcgcgg gcgctgtcgc cacgcgaaca cgagccgcgc gaacccaggt
    17941 ctcgtgaagc tcacgcgcac gtcgcgtgag cttccgacga ccaggagtgt tcgtactccg
    18001 tgacccgcta tttggtggcg ctcttgggta gggacgactc gtcgtcctcg ccaacccacg
    18061 tgtactctag cccgtcgccc atctcaccag cggaatcgcc acgatcgttc gtgtcgccga
    18121 ctccgagttg cccatccgag ttgtcacccc agcacttgat ccggccgctc tcgaggaggg
    18181 cacacgagaa cttctcgccc atgctccagc tcttgatgcg ctcgttcgct cccacgtcca
    18241 ccgggaccaa gctgctcatc tcccccggat cgtcgccgac gttgtcgttg ccgaccgagt
    18301 actcgcgccc aagcgcgccg tggtcattcg acccccagca cacgacttca cctttgcgca
    18361 gcatggcgca agtgctcatt ccgccagcct gaatactcag cgcgttcccg attcgggtcg
    18421 cactgagcag tgtgcccatc tgcgtttgat cttcgcctac cgacgcgacg ttctcatggc
    18481 cgagctggcc gttgccgttg agtccccagc acttcacgtc tcccgtctct gcgcgcgcac
    18541 acacgtgcgc gcggccgacc gcaatgctcg acactgggaa cgtgcccaac gacacatccc
    18601 gattggcaat cggcaccgct tctccgcgcg aggagacacc ggattgaccg tcactgttcg
    18661 ctccccagca gcgcacgcga gtgtcgagta acgcgcacgt caaatcgaag cctgctccga
    18721 tcgcccgcgg cgtctcgccg ccgagccccg ccggggcgaa cggcgtgtct gcctcttgcg
    18781 ggtagtagtc cgagccgaag ccggcgccgc attgagcctg cttgttgccg ccccaacaat
    18841 aaacttgctg gtccttcatc aacgcgcagg tgtgccagtc acccgccgtc agagcgatga
    18901 ccttggccgg cggcagccgg gtcgggacgg gcctcgtctc gaagctcgac agatgacccg
    18961 agtcaggcga ttcgcctagt tgcccgtagg agttgtctcc ccagcagtag accccgcccg
    19021 agctggcagc ggcacaaacg tgctgcctac ccaccgcgaa atccacgacc tcggtcatcg
    19081 ccacgggcaa gtctttgccc atctcaccag gattgtcgcc catatcgtcg acgttcccga
    19141 tcccgagttg cccgaactgg ttcgcacccc agcacttcaa gtcaccgttc ttgaagagtg
    19201 cgcagatggt gctgtgtcct gcttccaccc gaatcggctc ccactgacaa gtcgcggagc
    19261 aaccgtcacc gggagtcgta tttccgtcgt cgcaggcctc gccttcggcc ggcttcccat
    19321 caccacacgt tggattgcgg cagtcgccct cgaaacacgc gccatcgcac tgaatggacg
    19381 agaccgtgac taggtcatca ccgcatacgt gcagcacgtc tccggagcag aagcgttcgc
    19441 cgggtttgcg ttcctcgcaa ccttcagcga gcttcttgca cttgttgtcg gtggaatcgc
    19501 acaatgatcc cgcctcgcac tctcgctctt ctaggaatcc gcctctgcag atctcgtagt
    19561 tgctttgcgc gggccccgag cacgtggtca agccttcctt cttgcagggc ttgcgcggat
    19621 cgacgggtgc ccccgccatt ccaccttcgg gcatcggtac gctggtttcg ccgccggaac
    19681 caccttcgcc agcggttggg atgggagtta ctgtcgtgcc accctgaccg cccgccagga
    19741 ggggacacgg acgcgctccg ggcgcgtcgc acgcgacgaa cttatcgaca cagccgttgg
    19801 tgatgacgcc gccgagcacg agagcgcccc agagcaagag ccatgtaccc gtcgaaagtc
    19861 tcgcggccgt cgcatgccgt ctcatccgca ttactcctaa gttaattccc tgcaaaaaag
    19921 ctcagccgct cggttctcga atcgttgatt tgggtttccg gttggcttgt ttggcttctg
    19981 ctgctcatga ctcgggtctg catctgcgcg ccgttgctgg gcgaatcgtc gactgccgag
    20041 ggcgtgcatg gtgtcgaccg agatcacgtg cgtttcctct ccgaaaccaa ggccccccag
    20101 tagcgcgcca gcgacacagc gtcgagtcgt gtgtaggtgc gcgctgctcc cgggctcgtc
    20161 gagcctaccc tcggggctgg aagctatgtc ggtaaactat tgaattttat acatattgaa
    20221 taacacttcg ataagccgcc gtt
//
LOCUS       DASTKU010000032        12989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_674535, whole genome shotgun sequence.
ACCESSION   DASTKU010000032 DASTKU010000000
VERSION     DASTKU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12989
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674535"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1739
                     /locus_tag="VFQ61_01030"
     CDS             <1..1739
                     /locus_tag="VFQ61_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET9953050.1"
                     /translation="PGCVGGIEVDGRLETSSLQRDALETVSLFITEVAQAAPGQSGTT
                     GSASNADRVEVFCSGAAGCSAYKVCDTSVGGTACSALQPALASKQRAVVSRGSNITTS
                     DQVWLADASGLELAGTRVGPFDCGSGSSQTRSDCSIAPFSACGAPNLGSSSGSCSNGS
                     FPEPFAYRVYFTTNQHGVPESSCNRAACQALLSAIQSAHSSISFAIYGVRAQPDIVAA
                     LVAARERGVAVRGVVDSEDSACSSFGYSDTGALISALGDDRVRCDTGPGYSYIIHNKF
                     FVFDEASVWTGSTNISDTELGGEYNSDVAALIGSYKLAEIYQAEFDELWSGLCHNRKS
                     DNTEHVVDGSHFSDGTRLESYFSPTDRAVENAVIPLIAGATQTLDVAMFYFTNQAIAD
                     ALLAAGARGVRVRMVLDASGAANAASKHRQLCANGIEVKTENWGGKSHSKWAVADSAN
                     PAAAAVVFGSMNWTGAGDTQNDENTLYVRNSGLAASFAEEFARQWADLASVPTCTAVS
                     VEGSDSSRCTPSNDCGHICSSGSCCDGVDNDYDGRTDLQEEACGCGDGIDNDRDGYLD
                     GDDYDCKNLVDP"
     gene            1948..2673
                     /locus_tag="VFQ61_01035"
     CDS             1948..2673
                     /locus_tag="VFQ61_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953051.1"
                     /translation="MKRIQALTFTGTFALALIAVSACGETDGPALLQGGSGGRVGGSA
                     GQSADATGGRAPGSGGRTMGSGGRAGGATHVASGGDASETAGAAGEDSGGAAGADTAG
                     AGATARGGSSNAGGPSSSGGMSHVGGANAGGAAGAAGAAEAGAAGSGGAGNGGLCPIG
                     VPANKAACEDATSCAYPNMICVCGEADGQLRWTCTMQKCPPKAPSSGTDCKMMMRPGP
                     VQCEYDDGAICSCDMHGQWQCTP"
     gene            2986..4455
                     /locus_tag="VFQ61_01040"
     CDS             2986..4455
                     /locus_tag="VFQ61_01040"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET9953052.1"
                     /translation="MRLDEYEVDKRTYDETFEPNGKVRGVYRDLAARLSELSPQEFEQ
                     RRKLVEMLLRNQGVTFTVYSDDAGIEKVFPFDPVPRLIDADEWAKVERGLIQRVQALN
                     LFLADVYGAQRILDDGLVPPELIYRAGSFRREMVGMTPPQGIYTHVVGTDLIRDEKGV
                     LHVLEDNVRSPSGVSYVLECRDVMKRVFGVLFEHYGVRAIDDYTEWLRDSLLHVAPRS
                     TPQPNVVLLSPGMYNSAYFEHSFLARCMGVTLVEGRDLVVRDGVVCMRTTEGLRRVDV
                     IYRRIDDDFLDPVAFRPDSVLGCPGLFSAYRSGAVALANAPGTGVADDKAIYPFVPDM
                     IRYYLSEEPILPNVPTFIASRPDDRKYILEHLDELVVKQTDASGGYGMMIGPQASKEQ
                     REEFARRIEQTPRSYIAQRTIQLGKHPTLIDGHLEGRCVDLRPYILYGDKIRVIPGGL
                     TRVALRRGSLVVNSSQGGGYKDTWVLAKDAEENSRASVA"
     gene            4553..5485
                     /locus_tag="VFQ61_01045"
     CDS             4553..5485
                     /locus_tag="VFQ61_01045"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HET9953053.1"
                     /translation="MLSRVAEALYWMGRYLERAEHAARVLEAMRDVRLDLAEVDRDVA
                     DAQWHGALRALAMPDLPLGRLILDPAEATSLLCSVSRARENARQVREVISPQMWEELN
                     QRYWALRDAVGSRPNEDMLSQALTSIVSSCYLWDGVTDASMIRGEGWLYLKLGKFVER
                     TDGIARLLAVRLEDHANREASRSQEAGDSSGDNVEWLALIKSFDALDAYRKAHPKRVD
                     QTAVVTFMLFEVDFPRALRFSTNTAADLSRRLLRLHPESGRAVERHFGRLAARVEYAE
                     PATLEGTGSVRFLREVSADVNEASGHLQKTFFLS"
     gene            5624..6577
                     /locus_tag="VFQ61_01050"
     CDS             5624..6577
                     /locus_tag="VFQ61_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET9953054.1"
                     /translation="MLLSIDHKTELSYAQPISESVMELRMAPRTDSVQTLRTFGLAVG
                     PEAPVFEHVDWQGNRAHHFSIVDVHDKVVIVASSIVETHARRLRLEELTDTLPLAGLG
                     HRFEDFLMFHGPVQRDARLAQFAEELGLLGERRMAMVLATVTARLRELVEYRKGVTAS
                     STTVSDVLDHRKGVCQDFAHVALSLLRMLGVPSRYVSGYLFRPTSAELETHAWVESYL
                     PSSGWLGIDPTHGELASESHVAVAHGRSYADVPPNRGVYRGGAEEKIVVRVSMRRMDA
                     AGDAMATAPERPRVALRSDRSVRERELSLLALEQQQQQQQQ"
     gene            6655..9990
                     /locus_tag="VFQ61_01055"
     CDS             6655..9990
                     /locus_tag="VFQ61_01055"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET9953055.1"
                     /translation="MTIRVALRHTTRYNYDRRVRLGAQVVRLRPAPHSRTHIVSYAFK
                     VSPGVHFLNWQQDPQGNFLARLVFPEPTDHLEFDVDLVADMRTINPFDFFLEPSAEKV
                     PFRYEEASLGELGPYLEQTASGAKFDALVASLELEGKRTIDFLVELNQKVHRHVKYVI
                     RLEPGVQTPEETLELASGSCRDSAWLLVQVLRRLGLAARFASGYLIQLKADQKPLEGP
                     EGPEADFTDLHAWAEVYLPGAGWIGLDATSGLLAAEGHIPLACTPDPQSAAPITGSLD
                     ECEVEFEHRMSVTRIRETPRVTLPYTDPVWERIQNLGHRIDGDLEAHDVRLTMGGEPT
                     FVSIDDLDGVEWNFAALGEKKRELAGVLFRKLARRFAHDPLLHYGQGKWYPGEQLPRW
                     ALGCYFRKDGESIWRAQDLIAEDGGRYGIDERGAARFVEALARLLDVEPGNAIAAYED
                     PFHYMLTERRLPSNVTPLDNRLDDELERLRVAKVFEQGLGKVVGYTLPVLPLGGGQWR
                     SGQWFLRRELLYLLPGDSPMGYRLPLDSLPWVLESDYPYVHELDPFAERGPLPPYEPA
                     LIRRQRPGEAPPEGRGERKPAERESADWIVRTALCVEPRGGTLHVFLPPMVYLEDYLD
                     LVRRVEATAQELGMPVRIEGYTPPNDSRLEKFTVTPDPGVIEVNIHPAGSWDALVKNT
                     TALYEEARESRLGTEKFLLDGRHSGTGGGNHVTLGSYTPTDSPLLRRPDLLGSLVGYW
                     HDHPALSYLFSGLFIGPTSQAPRADEARHEASYELETALAEARRVSKNRDGVPPWLVD
                     RIFRNLLADVTGNTHRTEFCIDKLYSPDSASGRHGLLEMRAFEMPPHARMSLAQMLLV
                     RGLVSRFWQKPYESRVTRWGTSLHDRFMLPHFVWQDFRDVIRELQQAGYDFEENWFAP
                     HFEFRFPSIGSAAYDSVNVELRRALEPWHVLAEEAQAAGTVRSVDSSLERIQVKVQGA
                     IAGRHQLICNGARVPLHPTGVQGEAVAGVRFRAWQLPSQLHPTVGVNAPLQFSLIDTW
                     SERALGGCTLQVSHPGGRSYDRFPVNALEAEARRAALFAPIGHTPGRFDLSTIVDARS
                     PDYPLTLDLRRLSVQRPRGGEVW"
     gene            10001..12610
                     /locus_tag="VFQ61_01060"
     CDS             10001..12610
                     /locus_tag="VFQ61_01060"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET9953056.1"
                     /translation="MSTGAAGSLITPPESWGYAPAAGRFDEAFIKEGEVRPHFSGLFD
                     ALRPLSRDELDRRWSQAQRVIREHGVTYNVYGDPRGADRPWQLDPLPMVLSAEEWSRL
                     EAGLTQRARLLDAVLADLYGPQRLLKDGLLPPELVLGNPAFLRPLHGVPVPRNIRLQN
                     YAADLSRGSDGRFWVFGDRTQAPSGAGYALENRIVISRLLPQAFRDCRVERLAPFFRA
                     YRDGLMKLAQRRTDNPRIVLLTPGPYNETYFEHAYLARYLGFTLVEGGDLTVRDRVVH
                     LKTLGGLERVDVIVRRLDDSYADPLSLRPDSSLGVAGLVHAVRAGNVSIATPLGSGLL
                     ETPALSAFLPKIAKAWLGEELLVPTVPTYFCGDPNSLPYVLDRLETLVLKSSQLGPGL
                     REPVFGGDLSPTELAELSSRIRARPSAYVAQECLPLSTAPALTTSGIRPRSLSLRTFL
                     TRRDDNYVLMQGALTRIASSSESRVVSMQKGGGSKDTWVLAQGEPSSFTLLPPPDVRV
                     EVVRSGADLPSRVADNLFWIGRYVERAEVTTRLFRSLLARRGDDALGRAPEVSPLLST
                     LEDELELIRGMLTRSGTQLDAELLRLLLADQPPRTPAAGILSGYRAASAARDRISMDT
                     WRVLSQLSATVEQARQRGVSELSDGLEIVGSLMLGFSAFSGLVMENMTHGPGWRFLDI
                     GRRIERGSFMVRLLRATLVPAAHDAAVLDAALEVADSAMTYRSRYLGTLSMEPLLDLL
                     LTDETNPRSLAYQLAAIEGHVAKLPRSGRPLLTAEAKTVVRALSLVRLSELEKLARVD
                     PQTHQRVALSDLLAQLELALPVLADQLTLGYLAHAQPSFSLGSLAERVGSQELGVSNE
                     RSELAQRAGAEQV"
     gene            12610..>12989
                     /locus_tag="VFQ61_01065"
     CDS             12610..>12989
                     /locus_tag="VFQ61_01065"
                     /inference="COORDINATES: protein motif:HMM:NF019977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HET9953057.1"
                     /translation="MEKRVRYRVVHGTTYEYSEAVSICHNEARLVPRPTPLQRPGTRK
                     LSIEPTAAVLTEDTDYFGNVVHFFTLQEAHTRLSVTADFEVDLTVYEPPELANSPAWD
                     VVADAVLQDRTAEGLAALEYTFESP"
BASE COUNT         2193 a         3910 c         4423 g         2463 t
ORIGIN      
        1 tcccgggctg cgtggggggg atcgaagtgg acgggcgcct cgagacatct tcactgcaac
       61 gcgacgcact cgagactgtc tccctcttca tcacggaagt cgcgcaggct gcgcctggtc
      121 agtcgggcac tacgggcagc gcttcgaacg cggatcgggt ggaggtcttc tgctccggtg
      181 ccgccggctg ctccgcctac aaggtatgcg acacctccgt cgggggcacg gcgtgctcgg
      241 ctttgcaacc ggcgcttgcc tcgaagcaac gcgccgtcgt gtctcgcggt tcgaatatca
      301 cgacttcgga tcaggtgtgg ctggccgacg cttcgggact cgaactcgcg ggcactcggg
      361 ttggcccgtt cgattgcggg tctggctcgt cacagacccg gagcgattgt tcgatcgcgc
      421 cgttcagcgc gtgtggcgct cccaacctgg gcagcagcag tggcagctgc tcgaacggca
      481 gctttcccga accattcgcc taccgcgtct acttcaccac caaccagcac ggcgtgccgg
      541 aatcgagctg caatcgtgcg gcgtgccagg cgttgttgtc agcgattcag tcggctcaca
      601 gcagcatctc ctttgcgatc tatggtgtgc gtgcccagcc cgatatcgtc gcagccctag
      661 tggcggctcg agagcgcggc gtggcagtgc gcggagtggt ggactcggag gactccgcct
      721 gctcgagctt cggttactcg gacaccggtg cgttgatcag cgctctgggg gatgaccggg
      781 tgcgctgcga tactgggcca ggctacagct acatcattca caacaagttc ttcgtgttcg
      841 acgaggcgag tgtctggacg ggcagcacca atatcagcga taccgagctg ggcggtgagt
      901 acaactcgga tgtggccgcg ctaatcggct catataagct ggcggagatc tatcaggcgg
      961 agttcgacga attgtggtcg ggcctttgtc acaaccgtaa gtcggacaac accgaacacg
     1021 tggtggacgg cagccatttc agcgacggca cgcggctcga gagctacttc tcccctaccg
     1081 accgagccgt cgagaacgca gtgatcccat tgattgcggg cgccacccag acgctcgatg
     1141 tggccatgtt ctatttcacg aaccaagcca tcgctgacgc gctgctcgcg gcgggcgcgc
     1201 gcggggtcag ggtgcgcatg gtgctggacg cgagcggtgc cgccaacgcg gccagcaaac
     1261 ataggcagct ttgcgccaac ggcatcgagg tcaaaaccga aaactggggc ggcaagtcgc
     1321 atagcaagtg ggccgtagcg gattcggcca atcctgcagc tgccgcggtc gtattcggat
     1381 cgatgaattg gaccggagcc ggtgacactc agaacgacga gaacactctt tacgtcagga
     1441 acagcggctt ggctgcctct tttgccgagg agttcgcgcg gcaatgggcg gacttggcga
     1501 gcgtgccgac gtgcacggcc gtctccgtgg aggggagcga ctcttctcgt tgcacaccga
     1561 gcaacgactg cggccacatt tgcagtagcg gctcctgctg tgacggcgtg gacaatgact
     1621 atgacggcag gaccgatctg caagaagaag cctgtggctg cggagacggc atcgacaacg
     1681 atcgagacgg ctacctcgat ggcgacgact acgactgtaa gaacctggtc gatccgtgat
     1741 cgctccggag tagctcgcgt ctctctagaa tcttgcgttc tggtcgtact cgcgggtcgt
     1801 actcaccgcg gcgtgtaagt tttgcggcgg aggtcagaaa tccgaggggc gaactccact
     1861 gactctcaca tcaggagtgc gcccgagccg ggacgctgaa gtctacggct ttgctgtcgc
     1921 gcgcgctcac tcacccgaga gtgatgtatg aagaggattc aagcactgac cttcactggg
     1981 acgttcgcgc tggccttgat tgcggtgtcg gcatgcggtg aaacggacgg gccggcgcta
     2041 ctgcaaggcg gatccggcgg aagagtcggc ggctctgcag ggcagtcggc ggacgcgacc
     2101 ggcggccgtg caccgggctc cggggggcga acgatgggtt ccggaggccg agccggcgga
     2161 gcgactcacg ttgcctctgg tggcgacgcc agcgagacgg cgggagcagc cggcgaggac
     2221 tccggtggcg ccgcgggcgc tgacaccgca ggcgcgggcg cgacggcgcg cgggggctct
     2281 tccaatgcgg gaggtccttc gagttccggc gggatgagcc acgttggcgg agcgaacgcg
     2341 ggtggtgctg caggtgccgc aggtgccgcg gaagccggcg cggccggcag cggcggcgcg
     2401 ggtaacggcg ggctatgccc gatcggcgtg cccgccaata aggccgcgtg tgaggacgcg
     2461 accagctgtg cgtatcccaa catgatctgt gtgtgcggtg aagcggacgg gcagctgcgt
     2521 tggacctgca ccatgcagaa gtgcccgccc aaggcgccaa gcagtgggac tgactgcaag
     2581 atgatgatgc gccccgggcc cgtgcagtgc gagtacgacg acggcgcgat ctgcagctgt
     2641 gacatgcacg gtcaatggca gtgcacgcct tgagcgcggc tctccgcgag acagcttcgg
     2701 agagagacgc agaggattga cgggccccac ggcaaccagc ccgggttgcg gtgggggcct
     2761 gcgcggtgtc taagggggga gagggggccg gggctgcgct gatgccggat tgcgtgggcc
     2821 ggcgcgcccg ttgtcgagtg gccgccttga aagccgagac aggcttagac aactgagctt
     2881 cgtcagggat gagccgggtg taacgggacg gatctgccgc gcaacgaacg gagtaactcg
     2941 ccggaaacct ggcggaagca tggcgcgggg tcatgttggg gcggcatgag gctcgacgaa
     3001 tacgaggtcg acaaacgcac gtacgacgag actttcgagc cgaatggcaa ggtgcgcgga
     3061 gtgtatcgcg acctggccgc tcggctgtcc gagttgagtc cccaggagtt cgaacagcgg
     3121 cgcaagctgg tcgagatgct gctgcgtaac cagggcgtga cgttcacggt ctattcagat
     3181 gacgcgggca tcgagaaggt gttcccgttc gatcccgtgc cgcggttaat cgacgcagac
     3241 gagtgggcga aggtcgagcg cgggctgatt cagcgcgtgc aggcgctgaa tctgttcttg
     3301 gccgacgtgt acggggcgca acgcattttg gatgatggcc tggtgcctcc cgagctgatc
     3361 tatcgcgcag gctcgtttcg ccgcgagatg gtggggatga cgccgcccca gggcatttat
     3421 acgcacgtgg tgggcacgga cttgatccga gacgagaagg gcgtgcttca cgtgctcgag
     3481 gacaacgtgc gtagcccgag cggcgtgagc tacgtgctcg agtgccgcga cgtgatgaag
     3541 cgcgtcttcg gagtgctgtt cgagcactat ggcgtgcgcg cgatcgatga ctatacggag
     3601 tggctgagag actccttgtt gcacgtggcg ccgcgctcca cgccgcagcc caacgtggtg
     3661 ctgctgagcc caggtatgta caactcagcc tatttcgagc acagctttct ggctcgttgt
     3721 atgggcgtca cgctggtgga agggcgggat ctggtggtgc gcgacggcgt ggtttgcatg
     3781 cgcactacgg aagggctccg acgcgtcgat gtcatctatc ggcgcatcga tgacgacttt
     3841 ctggacccgg tggccttccg gcccgactcg gttttgggtt gtcccggctt gttctcggct
     3901 tatcgcagcg gcgccgtggc gctcgccaac gcaccgggca cgggcgtagc ggacgataag
     3961 gcgatctacc cgttcgtgcc ggatatgatt cggtactatt tgtcggaaga acccatcctg
     4021 ccgaacgtgc ccacgttcat cgcgtcgcga cccgacgatc ggaagtacat cctcgagcat
     4081 ctggacgagc tggtggtgaa gcaaacggat gcttccggcg gctatggcat gatgattggg
     4141 ccccaagcca gcaaagagca gcgcgaagag tttgctcgtc gtatcgagca gacgccgcgc
     4201 tcttacatcg cccagcgcac gatccagctc ggaaagcacc ccaccttgat cgatggccat
     4261 ctagaagggc gttgcgtgga tcttcgcccg tacattctgt atggcgacaa aatccgcgtc
     4321 atccccggcg gtttgacgcg cgtggcgctg cgccgcggca gcctcgtcgt caactcgagt
     4381 cagggcggcg gttacaaaga cacttgggtg ctcgccaagg atgcggaaga gaactctcgt
     4441 gcgagcgtcg cgtgacggtc gattgcaggt tcttgatgcg aggtatgtca ttgtcagtga
     4501 ggcgtgaagg cggcgcgttc gaggaaatcc caagcgcgct cgaggaaatc ccatgctgag
     4561 tcgagtggcc gaggcgctgt attggatggg tcgttatttg gagcgggccg agcacgcggc
     4621 gcgcgtgctc gaagccatgc gtgacgtgcg gctcgacctc gccgaggtgg accgtgacgt
     4681 ggcggatgca cagtggcacg gcgcgctgcg cgccttggcg atgccggatt tgccgctcgg
     4741 ccgcctgatc ttggatccgg ccgaagccac ctcgctgttg tgctccgttt cgcgcgcgag
     4801 agagaatgcg cggcaagtgc gcgaggttat cagccctcaa atgtgggagg agctgaacca
     4861 gcgttattgg gccttgcgtg atgccgtcgg ttcacggcca aacgaagata tgctgtctca
     4921 agcactcacg agcatcgtgt cctcgtgtta cctttgggac ggggtgacgg acgcgtcgat
     4981 gatccggggc gagggttggc tgtacctcaa actcggcaag ttcgtggagc gtaccgacgg
     5041 catcgcacgc ctgctcgcgg tgcggctcga ggaccacgca aatcgtgagg cgagccgcag
     5101 ccaggaagca ggcgatagct cgggtgacaa tgtcgagtgg ctggcgctga tcaagagctt
     5161 cgacgcgctc gacgcgtacc gcaaggccca ccccaagcgg gtggatcaga cggccgtcgt
     5221 cacgttcatg ttgttcgagg tcgatttccc gcgcgcgcta cgcttctcga ccaacaccgc
     5281 cgcagacttg tcgcgccgtc tgctgaggct tcaccctgaa tcggggcgcg cagtggagcg
     5341 gcacttcggc cgcctggctg cgcgcgtcga gtatgccgag cccgcgactt tggagggcac
     5401 cggcagtgtg cggtttcttc gcgaagtgag cgcagacgtc aacgaagcct cgggccacct
     5461 acaaaaaacc ttcttcctct cctgaggagc tctagttcta ttctgggcat cttgcggccg
     5521 cactggcccg gagccccaac tcccggaatc cccctccggg cattccacgc tcggaatcca
     5581 gctcggtcgc gaaccgagga cgcgatttag gaggaccaac ggtatgctgc tctcgatcga
     5641 tcacaagacc gaactttcct atgcccagcc gatcagcgaa tcggtgatgg aactgcgcat
     5701 ggcgccgcgc accgactctg tacaaaccct gcgcaccttc gggctcgcgg tggggcccga
     5761 agcaccggtg ttcgagcacg tggattggca gggtaatcgc gcgcaccact tctcgatcgt
     5821 cgacgttcac gacaaggtgg tgatcgtcgc gtcgtcgatc gtggagaccc acgcgcgtcg
     5881 gcttcggctc gaggagctga cggacacgtt gccgctggcg gggctgggcc atcgcttcga
     5941 agacttcctg atgtttcacg ggcccgtgca gcgggacgcg cgcttggctc agtttgccga
     6001 agagctgggg ttgctcggcg aacggcgcat ggcgatggtg ctcgcgaccg tcaccgcgcg
     6061 attgcgcgaa ctggtggagt atcgcaaagg ggtgacggcg agttcgacga ccgtgtcgga
     6121 cgtgctcgat catcgaaagg gcgtttgcca ggacttcgcc cacgtggcgc tctcgctgct
     6181 gcgtatgctg ggggtgccgt cgcgctacgt gtccggctac ttgtttcggc cgacctccgc
     6241 cgagttggag acgcatgcgt gggtggagtc gtacttgcct tcttcgggct ggctcggcat
     6301 cgacccgacg cacggcgagc tcgcttccga gtcccacgtc gcggtggcgc atggccggag
     6361 ttacgcggac gtgccgccca atcgaggggt gtatcggggc ggcgcggagg agaaaatcgt
     6421 ggtgcgcgtg agcatgcgtc gcatggatgc ggcgggggac gcgatggcga cggctccgga
     6481 gcggccgcgg gtcgctctgc gctcggaccg ctcggtgcga gagcgtgaac tcagtttgct
     6541 ggcgctcgaa cagcaacagc aacagcaaca acaataacca gaacctttca gcccgcgaat
     6601 tgcttggcaa cgttcaatcc gtctgccggg catcgctcta cctaagagaa gcccgtgacc
     6661 atacgcgtcg ctctgcgtca cacgacccgc tacaactatg atcgccgcgt gcgcctgggt
     6721 gcacaagtgg tgcgcctgcg gcccgcgccg cattcccgta cgcacatcgt gagttacgcc
     6781 ttcaaggtct cgcccggtgt gcacttcctc aactggcaac aagaccccca gggtaacttc
     6841 ctggctcggc tggtgttccc cgagcccacc gatcatctgg agttcgacgt ggatctggtg
     6901 gcggacatgc gcaccatcaa tcccttcgac ttcttcctgg agccgagcgc ggaaaaggtc
     6961 ccgttccgtt atgaggaggc gagtttgggt gaactcggcc cctatctcga gcagaccgcg
     7021 agcggagcga agttcgatgc gttggtcgcc tcgctggaac tcgagggtaa acgcaccatc
     7081 gattttctcg tcgagctgaa tcagaaggtg caccgccacg tcaagtacgt gattcgcttg
     7141 gagccgggcg tacaaacgcc ggaggagact ctggagctcg cgagtggctc gtgccgagac
     7201 tcggcctggt tgctggtgca ggtgctgcgt cgactggggc tcgcggcgcg cttcgcctcg
     7261 ggttacctga ttcaattgaa agccgatcag aagccgctgg agggccccga aggtccggaa
     7321 gcagacttca cggatttgca tgcctgggcg gaggtgtatc tgcccggtgc gggctggatc
     7381 gggctcgacg cgacgagcgg gctcttggcc gcggaagggc atattccact ggcttgcacg
     7441 ccggatccgc agagcgccgc gccgatcacg ggttcgctgg acgagtgcga ggtcgagttc
     7501 gaacaccgca tgtcagtgac tcgaattcgc gagacgccgc gggtcacgct gccgtatacg
     7561 gatccggttt gggagcgcat tcagaacctc ggtcatcgga ttgacggtga cctcgaggct
     7621 cacgacgtgc ggctcaccat gggtggagag ccaaccttcg tctcgattga cgatctggat
     7681 ggggtggaat ggaatttcgc cgcgctcggg gagaaaaagc gcgagctcgc gggcgtgttg
     7741 ttccgcaagt tggcgcgtcg tttcgcgcac gaccctttgc ttcattacgg gcaaggcaaa
     7801 tggtacccgg gagagcaact gccgcgttgg gccctgggtt gttatttccg caaagacggc
     7861 gagtcgattt ggcgcgcgca ggatctgatt gcggaagacg gtgggcgcta cggcatcgat
     7921 gagcgcggcg cggcgcgctt cgtggaggcg ctggcgcgcc tgctcgacgt cgagccgggc
     7981 aacgccatcg cggcttacga agatcccttc cactacatgc tcacggagcg ccggctcccg
     8041 agcaacgtga ccccactcga caaccgcttg gatgacgagc tcgagcgatt gcgggtggcc
     8101 aaggtcttcg aacaggggct cggtaaagtg gtgggctata cgctacccgt gttgccgctc
     8161 gggggtgggc agtggcgctc cgggcagtgg ttcctacgtc gcgagctgtt gtatttgctg
     8221 cccggcgact cgcccatggg ttaccgcttg ccgttggact ctctgccttg ggtgctcgag
     8281 tccgactatc cctatgtgca cgagctggat ccgtttgccg agcgcgggcc actaccgccc
     8341 tacgagccgg cactgattcg ccgccagcgc cccggtgagg ctcccccgga ggggcgtggc
     8401 gagcgtaagc cggcggaacg agagtctgcc gactggatcg tacgcaccgc gctttgtgtc
     8461 gaaccccgcg gcggcactct gcacgtcttc ttgccgccca tggtttacct ggaggattac
     8521 ctcgacctgg tgcgccgcgt ggaagccacc gcgcaagaac tcggcatgcc cgtacgcatc
     8581 gagggctaca cgccgccgaa tgactcgcgc ctggagaaat tcacggtcac ccccgacccc
     8641 ggcgtaatcg aggtgaacat tcaccccgcg ggtagctggg acgcgctcgt gaagaacacc
     8701 actgctcttt acgaagaagc gcgcgaaagc cggctcggta cggagaagtt cttgctggat
     8761 gggcgccaca gcggcaccgg cggtggcaac cacgtgaccc tcggcagcta cacgccgacg
     8821 gacagcccgc tgctccgtcg cccggattta ctgggtagtc tggtcggtta ttggcacgat
     8881 catcctgcgc tctcttacct gttttccggc ttgttcattg gcccgaccag ccaagccccc
     8941 cgcgccgacg aagcccgcca cgaagcgagc tacgagctcg agacggctct ggcggaggcc
     9001 cgccgtgtct ccaagaaccg cgatggagta ccgccgtggt tggtcgatcg catcttccgt
     9061 aatctgctgg cggacgttac gggcaatacc catcgtaccg agttctgtat cgataagttg
     9121 tattcgccag attcggcttc tggccggcac ggcttgctgg agatgcgcgc cttcgagatg
     9181 cctccgcacg cccgcatgag tctcgcgcag atgttgctcg tgcgcgggct cgtgagtcgc
     9241 ttttggcaaa agccttatga atctcgggtc acgcgctggg gcacatcgct ccacgatcgc
     9301 ttcatgttgc ctcacttcgt gtggcaagat ttccgggacg tgattcgcga gctccaacaa
     9361 gctggctatg acttcgaaga gaattggttt gccccacact tcgagttccg ctttccgagt
     9421 atcgggagcg cggcttacga ttcggtcaac gtcgagctgc gccgcgcgct cgaaccgtgg
     9481 cacgtgctag cggaggaagc gcaggcggcg ggcacggtcc gcagcgtgga ttcttctctc
     9541 gagcgcatcc aagtgaaggt tcagggcgcc atcgcgggca gacatcaatt gatctgtaac
     9601 ggggcgcgcg tgccgctgca tccgacgggc gtgcagggcg aagcggtggc gggcgtgcgg
     9661 tttcgggcgt ggcagcttcc gtcgcagctc catccaaccg ttggggtgaa tgcgccgctg
     9721 caattttcgc tgatcgacac ttggtcggag cgcgcgctgg gcggctgcac gctgcaggtg
     9781 tcccatcccg gcggcaggtc gtatgaccgg tttccggtga acgcgttgga ggcagaggca
     9841 cgacgcgcag cgctgtttgc cccaattggg catacgccag gtcgcttcga tctctccacg
     9901 atcgtggatg cgcgcagccc cgactatcca ctcaccctcg acttgcgaag gctgagcgtt
     9961 cagcggccgc gtggcggcga agtgtggtag gacctgagta atgtccacgg gagcggccgg
    10021 atccttgatt acgccaccgg agtcttgggg gtacgctcca gcggctgggc gcttcgacga
    10081 ggctttcatc aaagagggtg aagttcggcc gcatttttct ggcctgttcg acgcgctcag
    10141 gccgttgtcc cgggatgagc tggatcggcg ctggtctcag gcccagcgcg tgattcgcga
    10201 acacggcgtc acctacaacg tatatggcga tccgcgcggt gcggatcgcc cgtggcagct
    10261 cgatccgctg ccgatggtgt tgagtgcgga agagtggagt cggctcgaag ccgggctcac
    10321 gcagcgggcg cgcttgctgg atgccgtgtt ggcggacctg tatggtccgc aacggctact
    10381 caaggatggt ctgctgccac cagagctcgt gctcggtaat ccggcttttt tgcggcctct
    10441 gcacggcgtt ccggtgccgc gcaacatacg gctccagaac tacgcggcgg acctatcgcg
    10501 cggcagcgac gggcgtttct gggtgttcgg ggatcgcacc caggccccct cgggcgcggg
    10561 ctacgcgctc gagaaccgaa tcgtgatttc ccggctgctg ccgcaggcgt ttcgggactg
    10621 cagagtggag cgcctggccc cgttctttcg ggcttatcgc gatggcttga tgaagctcgc
    10681 gcagcgtcgg acggacaatc cgcggatcgt gctgctcacg cccggaccgt acaacgagac
    10741 ctacttcgag cacgcctatc tggctcggta tcttggcttc acgctggtcg aagggggaga
    10801 cctgacggtt cgcgaccgcg tggtgcacct aaagacactg ggcggcctcg agcgggtaga
    10861 cgtcatcgtg cgccgcttgg acgatagcta cgcagacccg ttgagtctgc gtcccgattc
    10921 ctccctcgga gtcgcggggt tggtgcacgc cgtgcgcgcc ggcaacgtga gcatcgcaac
    10981 ccccttgggt agtgggttgc tcgaaacgcc cgccctctct gcgtttctgc cgaagattgc
    11041 caaagcatgg ctgggcgagg agctgctcgt gcccacggtg ccgacgtatt tctgcggcga
    11101 cccgaacagc cttccgtacg tgctcgaccg gttggagacc ctggttctca agagttcaca
    11161 acttgggccg ggtttgcgtg agccagtatt cgggggcgat ttgtccccga ccgagctcgc
    11221 ggagctttcg agtcgaattc gggctcggcc ttccgcgtac gtggctcagg agtgtctgcc
    11281 gctttcgacg gcgccggccc tgaccacctc gggaattcgc cctcggagct tgtccttacg
    11341 gacgttcctc acgcggcgcg acgacaacta cgtgttgatg cagggggcgc tgactcgcat
    11401 cgcgagctcg agcgagtccc gcgtggtctc gatgcagaag gggggtggca gcaaggacac
    11461 ttgggtgctc gcccagggcg agccgagcag cttcaccttg ttgccgccgc cagacgtgcg
    11521 cgtcgaagtg gtgcggagcg gcgcggactt gccgagccgc gtggcggaca acctgttttg
    11581 gatcggccgc tacgtggagc gcgccgaggt gaccacgcgc ttgttcagat ctctgctcgc
    11641 gcggcgaggt gacgatgcct tggggcgagc tcccgaggtc tcaccgctgc tgtcgacctt
    11701 ggaagacgag ctcgagctga ttcgaggcat gctcacgcgc tcgggtacgc agctcgatgc
    11761 cgaattgttg cgtttgctgc tcgccgatca gccgccccga acgcccgcgg cggggatcct
    11821 atccggttac cgagccgcga gcgcggcacg cgatcgtatc tcgatggaca cttggcgagt
    11881 gttgagccag ctctcggcga cggtggagca agcccgacaa cgtggggttt ccgagctttc
    11941 ggacggtttg gagatcgtgg gcagcttgat gctggggttt tcggcgttct ccgggctcgt
    12001 catggagaac atgacccacg gtccgggctg gcgcttcttg gacatcggga gacgcatcga
    12061 gcgcggcagc ttcatggttc gcctgctgcg cgcgacgttg gtgccggctg cgcacgatgc
    12121 ggccgtgctc gatgccgcgc tcgaggtagc cgacagcgcg atgacctatc ggtcccgcta
    12181 cttgggaacg ctctcgatgg agcccttgct ggatttgctg ctcaccgacg agacgaaccc
    12241 gcgctcgctg gcttatcaac tcgcggcaat cgaggggcac gtggccaagt tgccgcgctc
    12301 ggggcggccg ctcctcaccg cagaggcgaa gacagtggtg cgggcgttgt ccctggttcg
    12361 cctgtcggag ctcgagaagc tggcgcgagt ggatccgcaa acgcaccagc gcgtcgcgct
    12421 gagcgatctg ctcgcgcagc tcgagttggc gctgccggtg ctcgccgatc agctcacgct
    12481 aggctatctc gcgcacgcgc agccatcgtt ctcgctcggc agtttggcgg agcgcgtggg
    12541 ctcgcaggaa ctcggtgtca gcaacgaacg atcagaactc gcgcagcgcg ccggggcgga
    12601 gcaggtttga tggaaaagcg agttcgctat cgcgtggttc acggtacgac gtacgagtac
    12661 agcgaagccg tatcgatctg tcacaacgaa gcgcggctcg tgccgcgtcc cacgcccctg
    12721 cagcgaccgg gaacgcgcaa gctcagcatc gagccgacgg cggcggtgct tacggaagac
    12781 accgactact tcggtaatgt ggtgcacttc tttacgctac aagaggcgca cactcggctc
    12841 tcggtcaccg ccgatttcga ggtggatctg acggtctacg agccgcccga attggccaat
    12901 tctccggctt gggacgtggt cgcggacgcc gtgctgcagg accgcaccgc ggaggggctc
    12961 gccgcgctcg agtacacctt cgagtctcc
//
LOCUS       DASTKU010000033         3531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1121030, whole genome shotgun sequence.
ACCESSION   DASTKU010000033 DASTKU010000000
VERSION     DASTKU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121030"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..944
                     /locus_tag="VFQ61_01070"
     CDS             117..944
                     /locus_tag="VFQ61_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HET9953058.1"
                     /translation="MTPAGQPSSAFQHVEDWAREYIRSTSLSYKIAPPPPPKLHREPG
                     TPERWDAPGRPRELILMAPGERQPRGGTLLEPERRARLFHSFWHHELQAAELMCWAML
                     AFADAEPSFRKGLLGICLDEIRHMAMYQAHIEALGYSIGAFPVRDWFWRRVPSCKVKQ
                     SYVALMGMGLEAANLDHAPSFAKAFRDAGDEEGARIQERVGKEELAHVSFATRWFKTW
                     TGGCDFTTWSELLPPPLSPWLMRADPMATEAREQAGMTPAFLGELAAYIPSHNGRPG"
     gene            952..1806
                     /locus_tag="VFQ61_01075"
     CDS             952..1806
                     /locus_tag="VFQ61_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953059.1"
                     /translation="MPFAWILNLDAELELSQPRYQASAAIRRRLEEHAQRAQGLLGAG
                     DITIDVLEANASMDASAFKARAWCPTRSAQARAAARGIELEPAPELSIIERVNHRLFA
                     HQLGGGLPGQRYVTQPAELDEVLAGAQWPLLFKRPFSFAGRGQQRLYRAPTAPEAAWL
                     AASIRTGGMIVEPLVQPIVEFSQHGWIEAHGQTRYGAPCVQSVSDRGVFQAARRATSA
                     DLRVHEQHALTERLDAVAVALRRAGYFGPFGIDAYRYDSRGRVGFCALSEINARYTLS
                     YPTGFSRA"
     gene            complement(1821..2726)
                     /locus_tag="VFQ61_01080"
     CDS             complement(1821..2726)
                     /locus_tag="VFQ61_01080"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9953060.1"
                     /translation="MCLLVFGACNKREPEPKGKSEPVAAAPTATAAPTSAAPSAAEPA
                     AAPAAPEPSGIPAPEDVAAAPKDAVKTESGLATKVLKGGTGKDRPADADQVKVHYTGW
                     TTNGKMFDSSVQRGEPATFGVNQVIPGWTEALKLMSVGEKRRLWIPAKLAYGVSPPPG
                     APAGDLTFDVELLEIVAAPKAPEDVKAAPATAKKTASGLAYRVLKKGTGKRHPTAADT
                     VTVHYSGWTTDGKMFDSSVQRGAPATFSLGQVIKGWTEGVQLMVEGEKTRFWIPGAMA
                     YGDTPRRPGAPAGMLVFDIELLQIR"
     gene            2970..>3531
                     /locus_tag="VFQ61_01085"
     CDS             2970..>3531
                     /locus_tag="VFQ61_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9953061.1"
                     /translation="MRSRPRSLGPLGLTLYNLIYWPYLLGTTILLFFPALLLYAVTPW
                     QRPRRWLHSYTSYWAAHYLAWAPYASVRVEGRANALHAGPVVFVVNHQSMVDILAVYA
                     THLPFLWVSKVENFYAPFLGWNMWLNGYIPIRRGYLPSILRMLRRCESRLKSGHSLCV
                     FPEGTRSKDGKLKQFYRGAFWIAARNG"
BASE COUNT          576 a         1160 c         1103 g          692 t
ORIGIN      
        1 cggctcgggc tgcccgcccg tgagctcgcc cacggatatc tgctcgaccg cgtgtgaggg
       61 ctcgtgtttc gagcggcaat tggcgcgccg ttcgaaatct gagctaatac tgccgcgtga
      121 cgccagctgg gcaaccatcg agtgccttcc aacacgttga agattgggcg cgtgaataca
      181 ttcgctcgac cagcctaagc tacaagatcg cgccgccgcc gccgcccaag ttgcaccgtg
      241 agcctggcac gcccgaacgt tgggacgctc caggtcgccc gcgcgaactc attctcatgg
      301 cgcccggaga acgccaaccc cgcggcggta cgctgctcga accggagcgc cgcgcacgcc
      361 tctttcattc tttttggcac cacgagctgc aggccgcgga gctgatgtgt tgggccatgc
      421 tggcgtttgc cgacgcggag ccaagctttc gcaagggcct gctcggaatc tgcttggacg
      481 agatccgtca catggccatg taccaagctc acatcgaggc ccttggctat tcaatcggcg
      541 catttccggt gcgggattgg ttttggcgcc gcgtcccgag ctgcaaagtc aagcagtcct
      601 atgtcgcgct gatgggcatg gggctcgaag ccgccaacct ggatcacgcg ccgagcttcg
      661 cgaaggcctt ccgagatgcg ggtgacgaag agggggcacg tatccaagag cgggtcggca
      721 aggaggagct cgctcacgtg agtttcgcca cgcgctggtt caagacttgg accggtggct
      781 gtgacttcac gacctggagt gagctcttgc cccctccgct atcgccctgg ctgatgcgcg
      841 ccgatccgat ggccaccgag gcccgcgagc aagccggcat gacgccggca ttcttgggcg
      901 agctggccgc gtatatcccg agccataacg ggcgccccgg ctgagccgcc gatgcccttc
      961 gcctggatcc tgaatctgga tgcggagctc gagctttcgc aacctcgtta tcaggcctcg
     1021 gccgccatcc gccgtcgact cgaagaacac gcgcagcgcg cccaaggtct gctcggggca
     1081 ggcgacatca cgatcgacgt gctcgaagcc aacgcgagca tggacgcgag tgcattcaag
     1141 gcccgcgctt ggtgtcctac tcgctcggcc caagcgcgcg cggcggcgcg tggaatcgag
     1201 ctcgaaccgg cgccggagtt gtcgatcatc gagcgggtca atcaccgcct gtttgctcat
     1261 cagctcggcg ggggcctgcc cgggcaacgc tatgtcacgc aaccggccga gctcgacgag
     1321 gtgctagccg gcgcacagtg gcccttgctg ttcaagcgcc cattctcgtt cgctggacgc
     1381 ggccagcagc gattgtaccg cgctcccacg gcccccgagg cggcttggtt ggcggcctcc
     1441 atccgaactg gcggaatgat cgtcgagccg ctcgtgcagc ccatcgtcga gttcagtcaa
     1501 catggctgga tcgaagcgca cggacagacg cggtatgggg cgccttgtgt tcaaagcgtg
     1561 agcgaccgcg gcgtcttcca ggctgcccgc cgcgcgacga gcgcggatct acgagtgcac
     1621 gagcagcacg cactcacgga gcggctcgac gcagtggcgg tcgcactgcg tcgagcgggt
     1681 tacttcggac cattcgggat cgatgcctac cgctacgact cgcggggcag agtcgggttc
     1741 tgcgcgctaa gcgagatcaa cgcgcgctac acgctgagtt acccgacggg cttttcacga
     1801 gcctgaacac gccgagttgc tcagcgaatc tgtagcagtt cgatgtcgaa cacgagcatt
     1861 cccgcagggg cgccgggacg gcgtggcgta tcaccgtagg ccatggcgcc tgggatccag
     1921 aaacgcgtct tctcgccttc caccatcaat tgcacgccct cggtccaacc cttgatcact
     1981 tggcccagcg agaaagtggc aggcgcgccg cgctgcacgg acgagtcgaa catctttccg
     2041 tccgtggtcc aacctgaata gtgcacggtc accgtgtccg cggccgtggg gtgacgcttg
     2101 cccgtgccct tcttgagaac ccggtaggcc agacccgaag cggtcttctt tgccgttgcg
     2161 ggcgcagcct tcacgtcttc gggtgccttc ggtgcggcga cgatctcgag cagttcgacg
     2221 tcgaacgtga gatcacccgc gggcgctccg ggcggcggcg agacaccgta ggccagcttg
     2281 gccggaatcc aaagacgccg cttctcgccg actgacatca gcttcagggc ctccgtccag
     2341 ccgggaatca cctggttcac cccgaatgtg gctggctctc cacgctggac cgaggagtcg
     2401 aacatcttgc cgttcgtcgt ccaacccgtg tagtgcacct tgacctgatc tgcgtcggcc
     2461 gggcggtcct taccggttcc ccctttgagc accttggtcg ctagccccga ctccgtcttg
     2521 accgcgtctt tcggtgccgc cgccacgtct tcgggagccg gaattccgct cggttcgggc
     2581 gctgcaggag cggcagcagg ctccgctgcg ctgggagccg cggacgtggg cgccgctgtc
     2641 gcggttggcg cggccgctac gggctcgctt ttccctttgg gttcgggctc gcgtttgttg
     2701 caggcaccga acacgaggag acacatcaag ggggcgagag caaacgagcg tcgagacatg
     2761 aaagtgtcgg gagtctttcg cagaaccccg tcgcttttca acctcaattc acgccgcagc
     2821 gcttcaaaac ccgagagtgc caattccaac actgctaacg cggccttcgc gccgcctctt
     2881 cggaactctt ggtactcttc gaacccccct cgcttcgaac aagcttgggc tcgcgcgcgc
     2941 ttgcgcccca agctaacgtt gccatcgcta tgcggtcgcg cccccgttcg ctgggcccgt
     3001 tggggctcac gctctacaat ctcatttatt ggccctattt gctgggcacg accatcctgc
     3061 tgtttttccc ggctctgctc ttatacgcgg tgacgccttg gcagcgccct cggcgttggt
     3121 tgcactctta cacttcgtac tgggcggcgc attacctggc atgggcgccg tacgcatccg
     3181 tccgtgtgga ggggcgcgca aatgcgcttc atgccgggcc cgtggtgttc gtcgtcaatc
     3241 atcagtcgat ggtcgacatt ctcgcggttt acgccaccca cttgcctttt ctttgggtga
     3301 gcaaggttga gaatttctac gcgccattcc tgggctggaa catgtggctc aatggttata
     3361 tccctatccg gcgcggctac ttgcccagca tccttcgtat gctgcgccgg tgcgaatcgc
     3421 gcctgaagtc cggccacagc ctgtgcgtgt tcccggaggg cacgcgctcc aaagatggca
     3481 agctgaagca gttctatcga ggggcgtttt ggatcgctgc gcgcaacggg g
//
LOCUS       DASTKU010000034         6975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_228793, whole genome shotgun sequence.
ACCESSION   DASTKU010000034 DASTKU010000000
VERSION     DASTKU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228793"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1069
                     /locus_tag="VFQ61_01090"
     CDS             191..1069
                     /locus_tag="VFQ61_01090"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HET9953062.1"
                     /translation="MAFKYWLSIVVLAWGAAAAGCAAEFAESPDPECAKGQQIRTQEP
                     SSGSEARAVSVAIPFDDPNGIGSAGEQETRELIVRNKEYVKLFGHKPPPEVDFAAGDV
                     VIFYSAGVQRTGGYVASLPRVELRGQFLSITTRLESPGPGCAVTLALTKPFVLAKVRV
                     RGHEVRAQFKADDVVRDCRPKGCEGVECAKGERCELHEVVCVRAPCDPVPVCVADVNG
                     PFCGGIAGRPCPGAGSCVDDPDDTCDPNEGGADCGGMCVCAVQHNCGAGQVFDSSPGV
                     CACVPQKEADPAGSED"
     gene            complement(1133..4264)
                     /locus_tag="VFQ61_01095"
     CDS             complement(1133..4264)
                     /locus_tag="VFQ61_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9953063.1"
                     /translation="MKLTDIFIRRPVLAIVVNLVIIIAGLQAIRSLNVRQYPKLESAT
                     VTVRTPYVGANSDLVRGFITTPLERAIAAADGIDFIESQSTQGLSTINVRLKLNFPAA
                     SALADISARVNQVRADLPPEAEIPAINIEPSDAQVAAMYLSFGSNILEDNQVTDYLIR
                     IVQPRLSAIEGVQRAEILGGRAFAIRAWLKPERMAALNVSPAQVRQALSANNYLSAVG
                     QTKGQLIQVNLTASTDLKTVDEFKRLVIRERDGSLVRLEDIADVILGADNYEQDVRFS
                     GKREVFMGVWVLPNANSLDVIRRVRTEVDTLRAELPTGMRATVAFDSTNYINNAVQEV
                     TRTLTETVLIVVVVIFLFLGSLRTVLVPLVAIPVSLIGAVFLMQVMGFTLNLLTLLAV
                     VLAVGLVVDDAIVVVENVERNIRDGKTPMQAAVLGARELFGPIVAMTITLAAVYAPIG
                     FQGGLTGALFREFAFTLAGAVLISGVVALTLSPMMSSRLLVPDHNPGWLVRNINAGFE
                     GLKRRYTQILDWTLRTRGAVYAVWIVLTLAVVPMYMLSPQELAPNEDQGIVFGAIDVP
                     ANATLEQIVPNTAEVNRIFQSVPEFQQSFQITFPTGGFGGAMMKPWKERSRSIFPIHG
                     ELSQKTSSVTGIRAPVFLPSALPSAGFFPVEFVIASTASHEELLRFAQSLVEEAVKSG
                     EFAFPPITDVRIDQSKGEIVIDREKVASMGLSMQSVGADLSAMLSGNFVNRFNIDGRS
                     YKVIPQVERAGRLTPDQLASIHVTGPTGELIPLGSIASVVRGIEPRSLNRFQQLNAVK
                     ISGVAPRSLDRGLHVLEDAAAKILPPGYRIDYTGESRQLRQEGGKFLPALGLSIVLIF
                     LALAAQFNSFRDPFVVLAGSVPLAMFGALIFTFLKFAGPPGMSFALTEGWTSTLNIYS
                     QVGLVTLVGLVAKNGILIVEFANVQQSLGMSKLEAVRAAASTRLRPILMTTVATVAGH
                     FPLTLVTGPGAIARNSIGIVLVGGMAIGTFFTLFIVPSLYMLIAQTHRQPGTSTETPS
                     SRAPAEAPSWAAT"
     gene            complement(4275..5399)
                     /locus_tag="VFQ61_01100"
     CDS             complement(4275..5399)
                     /locus_tag="VFQ61_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9953064.1"
                     /translation="MRYALIILGLIVMLAALGGIKATQISSLIAAGKAMQEAGPPPES
                     VSVATAEQRVWETTLHAIGSVVSVKGVALSNDAPGVVSRIAFDSGAEVKKGQILVELD
                     SKVERAQLSSLRARRWLAERSRERTKSLVQTGSLAQAELDTAESTLNGLNADVAALEA
                     QIERKIVRAPFAGKLGIRAINLGQYLAPGTTLTVLESNQPTSVDFTLPQKEIDRVRPG
                     LPIRVFAESNPTPIAEGTLETVEPALEAVTRSLKLRARVPHDEGKLRPGMFVNIQVVL
                     PEQNTRVIVPQTAVIHAAYGDSIFSVEPDPKDGKRMLARQHFVRLGEERGDFVAILEG
                     LNAGQRVVSSGAFKLRNGARVVLSDQAQTKPELDPRPQNR"
     gene            6298..>6975
                     /gene="rpiA"
                     /locus_tag="VFQ61_01105"
     CDS             6298..>6975
                     /gene="rpiA"
                     /locus_tag="VFQ61_01105"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET9953065.1"
                     /translation="MNAEQQKRHAARTALEFLPDAGILGLGTGSTTRYFIEGVSELVK
                     NGRDYSGVPTSEQSRLLASSLGIPLLGDDGPWDVLLCVDGADEVSEELDLIKGGGGAH
                     TREKIVNFASRRNVIVVDESKLSRRLGERRGVPVEVLPFAHHETAHLIGAYGTAVLRY
                     QGSGPLRTDSGNLIYDLECGPISDPRSLDMALKTIPGVVETGLFCGRADAVIVARTSG
                     IEVLKRAA"
BASE COUNT         1339 a         2030 c         2323 g         1283 t
ORIGIN      
        1 cctttcgggt ggcgcgcggg caagcgggcc gcgcttcgtg acgcgtaaag cgatccgacc
       61 tcggacatat tcgatccgag cgagtagttc ggactttgtg tgatccaggc actcgaatca
      121 cgcgtgcaaa ctcacgcgga cggacgcaca atggtcgtcg cggttgctgg agaagaggga
      181 ggtattcgca atggcattca aatattggct atcgattgtc gtgttggcgt ggggcgcagc
      241 cgccgccggt tgcgccgctg agttcgccga aagcccagat ccggagtgcg cgaaggggca
      301 gcaaatccgc actcaggaac cctcttcggg gagcgaggct cgagccgttt cggtggcgat
      361 tccgttcgat gatccgaacg ggatcggcag cgcgggcgag caggagactc gagagctgat
      421 cgtgcgtaac aaagagtacg tgaagctgtt tgggcacaag ccgccgccag aggtggattt
      481 cgcggcgggg gacgtagtca tcttctacag cgcgggggtg caacgtacgg gagggtacgt
      541 tgcatcactc ccgcgggtag aactgcgcgg ccaattcctg agcatcacga cgcggctcga
      601 gtccccgggg ccgggctgcg cggtgacgct cgcgctcacg aaacccttcg tgctggcgaa
      661 ggtgcgcgtg cgggggcacg aggtgcgcgc gcaattcaaa gcggatgacg tggtgcgtga
      721 ttgtcgaccc aagggttgtg aaggggtgga gtgcgcaaag ggggagcgct gtgagctgca
      781 cgaggtggtg tgcgtgcgcg cgccgtgtga cccggttcct gtctgcgttg ccgatgtgaa
      841 tgggcccttc tgcgggggca tcgcggggag gccttgtccg ggggcgggca gctgcgtcga
      901 cgatccggac gacacgtgcg atccgaacga aggcggggcg gactgcggag ggatgtgcgt
      961 ctgcgccgtg caacacaact gcggagcggg acaggtgttc gacagctccc cgggcgtgtg
     1021 tgcttgcgta ccgcaaaaag aagccgatcc cgcgggtagc gaggactgag cccactgctc
     1081 gggtcctact atccggggat cggctgaggg gtggcggggg atggggtaac ggctaggtcg
     1141 cggcccagct gggcgcttcc gcgggagctc tgctgctcgg agtttccgtg gaggtgcccg
     1201 gctggcgatg ggtttgcgca atcagcatgt aaagcgacgg cacgatgaac aacgtgaaga
     1261 acgtgccgat cgccatgcct cccacgagca cgatgccgat cgagttacgc gcaatggcgc
     1321 ccggccccgt aacgagcgtg agcgggaagt gacccgccac cgtcgcgacc gtggtcatga
     1381 ggatcggacg caagcgcgtg gaagcagccg cccgcacggc ctcgagcttg ctcatgccca
     1441 agctttgctg cacgttcgcg aactcgacga tcaggatgcc gttcttggca accaaaccga
     1501 ccaacgtcac gagcccaacc tgggagtaga tgttgagcgt cgaagtccag ccttcggtga
     1561 gcgcgaagct catgccaggc gggcccgcga acttgagaaa cgtgaagatc agtgcgccga
     1621 acatggcgag cggcaccgag cccgcgagca ccacgaatgg gtcgcggaag gagttgaact
     1681 gcgccgccag cgccaagaaa atcagcacga tcgagagccc gagcgccggc aaaaacttgc
     1741 cgccctcttg ccgtagttga cgcgactcac cggtgtaatc gatgcggtaa ccggggggca
     1801 gaatcttggc cgccgcgtct tcgagcacgt ggagaccgcg atcgagcgag cgcggagcca
     1861 caccggagat cttgacggcg ttcagctgtt ggaagcggtt caaggaacgc ggttcgatgc
     1921 cgcgcaccac gctcgcgatc gacccgagcg ggatgagctc accggtggga ccggtgacgt
     1981 gaatgctggc tagctgatcg ggcgtgagcc gccccgcgcg ctccacttgc ggaatgactt
     2041 tgtagctgcg accgtcgatg ttgaagcggt tcacgaagtt gccgctgagc atcgccgaca
     2101 ggtctgcgcc gaccgactgc atgctcaggc ccatcgacgc gaccttctcg cgatcgatca
     2161 cgatctcgcc tttgctctgg tcgatgcgta cgtccgtgat cgggggaaac gcgaactcgc
     2221 cgctcttgac cgcttcttcg actagcgatt gcgcaaagcg cagcagctcc tcgtggctgg
     2281 cggtcgaggc aatgacgaac tcgaccggga agaagcctgc gctcggcaaa gccgacggca
     2341 ggaagacggg cgcccgaatc cccgtcaccg aagaagtctt ttgactgagc tcgccgtgga
     2401 ttgggaagat gctgcgtgag cgctctttcc agggcttcat catggcgccc ccgaaaccac
     2461 ccgtggggaa ggtgatctgg aagctctgtt ggaactccgg cacgctctgg aaaatgcgat
     2521 tcacctcggc cgtgttgggt acgatttgct cgagggtggc gttggcgggc acgtcgatcg
     2581 cgccgaagac gatgccttgg tcttcgttgg gagcgagctc ctgcggggac agcatgtaca
     2641 tcggcaccac ggcgagcgtg agcacgatcc acactgcgta gacggccccg cgggtgcgga
     2701 gcgtccagtc cagaatttgg gtataccgcc gcttcaggcc ctcgaagcca gcgttgatgt
     2761 tgcgcaccag ccagcccgga ttgtgatccg gcaccaacaa gcgcgacgac atcatgggtg
     2821 aaagagtgag cgcgactacg ccagagatga gcacggcgcc agccagcgtg aacgcgaact
     2881 cgcggaacaa ggcgccggtc aagccgccct ggaagccgat cggcgcgtac accgcggcca
     2941 gcgtgatggt catggcgacg atcggaccga acagctctcg cgcgccgagc acggcggcct
     3001 gcatcggtgt cttgccgtca cggatgttgc gctcgacgtt ttcgaccacg acgatcgcgt
     3061 catccacgac cagacccacc gccagcacga ccgccaacaa cgtcagcagg ttcaaggtga
     3121 accccatcac ctgcatcaag aacacggcgc cgatcagcga gaccggaatg gcaaccagcg
     3181 gcacgagcac ggtgcggagc gagcccaaga acaggaagat caccaccacc acgatcaaga
     3241 ccgtctcggt gagcgtgcgc gtcacctctt gcacggcgtt gttgatgtag ttcgtggaat
     3301 cgaaggccac ggtggcgcgc atgccagtcg gcaactctgc gcgcagggta tcgacctcgg
     3361 ttcgcacgcg acgaatcacg tccagggagt tggcgttcgg caacacccag acgcccatga
     3421 acacctcgcg cttaccggag aagcgcacgt cctgttcgta gttgtcggcg ccgaggatta
     3481 cgtcggcgat gtcttccagg cgtaccaagc tgccgtcgcg ctcgcgaatc acgaggcgtt
     3541 tgaactcgtc cacggtcttc agatcggtgc tcgccgtcag attgacctgg atgagctggc
     3601 ctttggtttg gcctactgca gagaggtagt tattggccga cagggcctga cggacctggg
     3661 ccgggctaac attcagagcc gccatccgct cgggtttgag ccaagcccga atggcgaaag
     3721 cacgaccacc cagaatctct gcgcgttgca cgccttcgat ggcggacaag cgcggctgta
     3781 cgatgcggat caggtagtca gtgacttggt tgtcttcgag gatgttcgag ccgaagctca
     3841 ggtacatcgc ggcgacctgc gcgtccgaag gctcgatgtt gatggccggg atctcggcct
     3901 cgggcggcag atcggcgcgc acttggttga cgcgagcgct gatgtccgcg agcgccgagg
     3961 cagccgggaa attgagcttc agccggacgt tgatggtcga gagcccttgc gtgctctgtg
     4021 actcgatgaa atcgataccg tcggccgcgg cgatcgctcg ctcgagcggg gtggtgatga
     4081 agccacgcac cagatccgag ttggccccta cgtacggggt gcgaaccgtg acggtcgcgc
     4141 tctcgagctt ggggtattgg cggacgttca acgaacggat cgcctgcaag cccgcgatga
     4201 tgatgaccag gttgacgacg atcgcgagga ccggtcgtcg gatgaagatg tccgtgagct
     4261 tcatggagtg agcctcagcg attctgcggg cgtgggtcga gctcgggctt ggtttgcgcc
     4321 tgatcgctca acactacgcg cgcgccgttg cgcagcttga aagcgcccga gctgaccacg
     4381 cgttgcccgg cattgagccc ttccaggatg gcgacgaagt cgccgcgttc ctcgccgaga
     4441 cggacgaagt gctgacgtgc cagcatgcgc ttcccgtcct tcggatcggg ttcgacggaa
     4501 aagatggagt ccccgtaggc cgcgtgaatc accgccgtct ggggcacgat cacgcgggtg
     4561 ttctgctccg gcagcacgac ttgaatgttc acgaacatgc cagggcgaag cttgccttcg
     4621 tcgtgcggca cgcgggcgcg aagcttgagg ctgcgggtca cggcctccaa agccggttcc
     4681 accgtctcca aagtgccctc ggcgatcggc gtcggattgc tctcggcgaa cacgcggatg
     4741 ggcaagccgg gacgaacgcg gtcgatctct ttctggggca acgtgaagtc taccgatgtg
     4801 ggctggttcg actcgagcac ggtcaacgtg gtgcccggcg ccagatactg acccaggttg
     4861 atggcgcgga taccaagctt gcccgcgaac ggagcgcgca cgatcttgcg ttcgatctgc
     4921 gcctcgagag ccgcgacgtc cgcgttcaga ccattgaggg tcgactctgc agtgtcgagc
     4981 tcggcttgag cgaggctgcc cgtttgcacg aggctcttgg ttcgctcgcg cgaacgttcc
     5041 gcgagccaac gccgagcgcg caacgagctg agctgagcgc gctccacttt gctgtcgagc
     5101 tcgaccaaga tttgaccctt cttgacctcg gcgccggaat cgaaggcaat gcgcgagacc
     5161 acgcccggcg catcattgga gagggccact cccttcacgg agacgacgct gccaatcgcg
     5221 tgcagcgtgg tttcccaaac tcgctgttcg gcggtcgcta cgctcacgct ctcgggaggc
     5281 gggccggcct cctgcatggc tttgccggcc gcaatcaagg acgagatctg ggtggctttg
     5341 atgccgccga gcgcggccaa cataacgatc aatccgagga tgatgagtgc ataacgcata
     5401 ggagtaactc cgaaagcccg aacgagaaag gttgccgctc ggatgaagtg cttgttcgac
     5461 gctgcttgtt cggctctgct tgttcgacgc tgcttggctc tgcttgttcg acgctgcttg
     5521 gcttattcgg cgctgcttgt tcaaacgggc tcattcaagc tggcttagcc aacctggttc
     5581 gttccaacct gatcattcgc gctgacattc ggtggtgctt gttcgcttcg gcggagctga
     5641 tttggcgatt tgattcggct gtgctcgtcc gatggcgttg actcgctcta ccgagagggt
     5701 gaagtcaggc agcaaagtcg atgaaccagc gagcggtagc tcgaatcgta atgagttgaa
     5761 tcggaaacgc ggccgataaa tacggccagg agtttgagcg agcgattttg cccagcggtc
     5821 cagccagcta ggaaacatct cgcgctagat gcacgatgcc agctgccgag ggtacctaga
     5881 gcaggtccgt gcgaagcgct accccacttc cgggcagtcg attgcgaaag ttcgatgaag
     5941 ccgctcgctc ctaccaaaac ctgccgcgag atcgaaacaa actctgcacg caatgtgaac
     6001 tggactgagc cgactagtgc attgcgaagc gtcgagttgc gatcccaagc atcttttttg
     6061 tccgtcccag cccgcccgtg ctctgcgcgt ctacaccgct acggagcggc gacagcatag
     6121 ccgtttcgcg ggaaaaagta ccgaagacta gctcggagct cccgtttcag actgagatgt
     6181 aaagcgtaca catgtccgct cgtggttgga ttgggctgcc gttttgacag atttcacgag
     6241 ttggttgcgg gccaggggcg cctgatttag aggaagcgcg caaacggacg gcaggcaatg
     6301 aacgcggaac agcaaaaacg tcacgcagca cgcacagctc tcgagttctt gccggatgcc
     6361 gggatcctcg gactcggaac cggatcgacg acccgttact tcatagaagg cgtgagcgag
     6421 ttggtgaaga acggacgcga ttactcgggc gtccccacta gcgagcagag ccgcttgctg
     6481 gccagtagct tggggatccc gttgctgggt gatgatggcc cctgggacgt actgctctgc
     6541 gtggacggcg ccgatgaagt gagcgaggaa ctcgacttaa tcaagggtgg aggcggcgcg
     6601 cacacccgcg agaagatcgt gaatttcgct tcgcgacgca atgtgatcgt cgtggatgaa
     6661 agcaagctga gccgccggct tggtgagcga cgtggagtgc cggtggaggt gctgcccttc
     6721 gcgcatcatg aaaccgctca cctgattgga gcctacggaa cagcggttct gcgataccaa
     6781 ggttctgggc cgcttcgtac ggactctggc aacctaattt atgacctcga gtgtggtccc
     6841 atctctgatc cacgcagctt ggatatggct ttgaagacga tcccgggtgt ggtcgagacc
     6901 ggcctatttt gcggtcgagc agacgccgtg atcgtggcac gaacgagtgg gatcgaagtc
     6961 ctgaagcgag ccgcg
//
LOCUS       DASTKU010000035        10532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1121203, whole genome shotgun sequence.
ACCESSION   DASTKU010000035 DASTKU010000000
VERSION     DASTKU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121203"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFQ61_01110"
     CDS             complement(<1..242)
                     /locus_tag="VFQ61_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953066.1"
                     /translation="MPRLSLRQLMLAFVVFTAFPAQAQYTRDAAANRKIDEAINTHYL
                     ATDFDKAEAVLTGTIKACDDKCSPQTLAKAWMYVGIV"
     gene            complement(352..1422)
                     /locus_tag="VFQ61_01115"
     CDS             complement(352..1422)
                     /locus_tag="VFQ61_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9953067.1"
                     /translation="MFSCAEAPRLRGRVAGLEKVVEQAERNGAVRCAPIELALARSQL
                     KFASLELDQGFPLRAQAHLVRAEPQARAALALSPPEYCATRAYVQIAGDRDGDGFPDD
                     RDRCPEQPENLNNFEDADGCPDDPDTDGDGVSDSRDACDLEAEDRDSYLDDDGCPDLD
                     NDVDGALDPDDKCPVAAEDPDGYEDADGCPDPDNDGDSVPDVKDQCPNEIGSTTSEPL
                     GCPQKPALVLVTDCEVKITQQIHFEFNQDRIRRESYPILDAVAEVLSKNSAIKVEVQG
                     HTDNVGSPAYNRDLSNRRAGSVRKYLVARGITPARLSSVGYGMERPLVDNTSEQNRSL
                     NRRVQFVRTEGVKEGCPKTRTP"
     gene            complement(1605..2150)
                     /locus_tag="VFQ61_01120"
     CDS             complement(1605..2150)
                     /locus_tag="VFQ61_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HET9953068.1"
                     /translation="MRVVQAAFYREWPVQREARPSTVSETTRRAAGQSASAVTHIADR
                     SDGKPRASRNIDGSTRDRTDASPIAALSGSTRSAHPALWFWRLAWLGLSVSPLLGCGH
                     GLYALSINQAAAKLEEARELDAEHLAPYEFYMAKEHLAKARSEAAEAEYGDALDLSDA
                     AAEYAEKASRLARDAHRGAGR"
     gene            complement(2225..2365)
                     /locus_tag="VFQ61_01125"
     CDS             complement(2225..2365)
                     /locus_tag="VFQ61_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953069.1"
                     /translation="MKALISALSGKAAFASRLASREAGAQACAKLLPKILLQRSPGFR
                     AE"
     gene            complement(2467..3105)
                     /gene="lexA"
                     /locus_tag="VFQ61_01130"
     CDS             complement(2467..3105)
                     /gene="lexA"
                     /locus_tag="VFQ61_01130"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET9953070.1"
                     /translation="MQGLTKRQEQTLDFIRKSIEERGYPPTLREIGEYMGIRSTNGVN
                     DHLRALERKGYLRREDMKSRALKLVEDSPPASARTDEEDLIEVRVLGRVAAGLPLFAE
                     ENVIDTLRIDRMLVRGGREVFGLKVTGDSMIDAGIFSGDYIFVKRQGQADRGDIVVAL
                     IGDEATVKYYFPERDYVRFQPANAQMAPILVRATDFKPTMLLGKVIGVYRSL"
     gene            complement(3400..3882)
                     /locus_tag="VFQ61_01135"
     CDS             complement(3400..3882)
                     /locus_tag="VFQ61_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9953071.1"
                     /translation="MLSQPERAERVEQLARVDLFAGLKPQALELIAQVANEEIHRVDT
                     KVFRYGEAGDKLYLILQGKVRISRDVAGMGEEALAVLGPGEAFGEMALLDEAPRSADA
                     HVHEECRLLVLSKDAFDDLLFLHKDLAYEVLWNVVKQLVGRLRNTTDKLTFLSISSRF
                     "
     gene            complement(3903..4826)
                     /locus_tag="VFQ61_01140"
     CDS             complement(3903..4826)
                     /locus_tag="VFQ61_01140"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9953072.1"
                     /translation="MTQPVRSRNPVALTSIKGSGPTELAARKQLGDPLAPIIALGRKQ
                     ARIGAVIGLLGALALHGAPGSQAFRSFNDLRTFTQDVISIVRHRLGSELEVEAEKPPP
                     PPPPPEPEPPKPEPEPPPVAKAAPPPKAAAEAPPPPPAPAEAGKVLTQDPDPNAPVDL
                     TGEGFVTGTADRYAGGITATRGTSKVAVRDTNATPGGVPGGTGVKLAPPPPAEDRSRP
                     AAPDRNANWRDCPFPPDADIEGINEARVRVSVVVGPDSRPKSVTVLDDPGHGFGRVTR
                     DCVLRKPFIAGLDRSGQPVTQTAVFWIRFTR"
     gene            complement(4833..5276)
                     /locus_tag="VFQ61_01145"
     CDS             complement(4833..5276)
                     /locus_tag="VFQ61_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9953073.1"
                     /translation="MAGGAADDDDGLISGINVTPLVDVTLVLLIIFMVTAKIIVSQGM
                     PMDLPKAASGESLQTVFSVELSADGKTRVDSAVIPNDEAIFPLARDSKNKNKELRAVI
                     RADRKVEHGRVIHVLDLLKRAGIAKIAFAVSPLAEGTPVDTAPTK"
     gene            complement(5280..6035)
                     /locus_tag="VFQ61_01150"
     CDS             complement(5280..6035)
                     /locus_tag="VFQ61_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9953074.1"
                     /translation="MDLVPWLQKLMTNFGAGWVMWLLILLSVVSVAIMLERGWFYWSL
                     RDDIGALAQKLRDQLRNGDLEGARASLERSPSAEAAVVVAGLAEADRGAKAADEAMQG
                     AMALQRIKLERRLAFLATLGNNATFIGLFGTVIGVVEAFEQLGKQGSGPAAKAAAAAA
                     ATAPQEVMAAIAEALVATAVGLAVAIPAVVMFNYYQRLSKAVLANTAALSHVLLSYLA
                     NPTEPLRSSASKLDKAERVRAAKINAADAEEEN"
     gene            complement(6487..7989)
                     /locus_tag="VFQ61_01155"
     CDS             complement(6487..7989)
                     /locus_tag="VFQ61_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IG family 5'-nucleotidase"
                     /protein_id="HET9953075.1"
                     /translation="MGDIPVTAIPSPVSDPRQLILPIAGVSLPPPNGHAICRKDRVFV
                     NRDLRLAGIQWFGFDMDYTLAIYRQDEMDRLSVELTVERLIRRGYPSYLKHLEYDTRF
                     PIRGLLVDKRHGHVLKMDRYKVVHYGYHGMRRLPREELAELYHHKRIRPHTPRYHWID
                     TLFALSEVTSYAAIVDALEKRGEKVDFDRLFSDVRSAIDEAHADGAVYAAITADFPRY
                     IERDPELARTLHKLRSAGKHLFLLTNSPFHYTHSMMNYLLGEAMPEYPTWRHFFDIVI
                     CAAAKPRWFQEGRPLLERDGENLRHVRGQLERGRVYEGGNLADFERLASVQGSSVLYV
                     GDHIYGDILRSKKESSWHTAMIIPELDAEVEAYEVCAEDLARQRVLEETRDRLEDELR
                     YYQTRYKELMRNPAEFGESERARVKRALDRIRTELRSSLSEHETLAERISTRFHPYFG
                     SLLKEQNEMSSFGLQVDLYADIYMRRVSCLKAYSPQQFFRSPYDLMPHEL"
     gene            complement(8115..9242)
                     /locus_tag="VFQ61_01160"
     CDS             complement(8115..9242)
                     /locus_tag="VFQ61_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HET9953076.1"
                     /translation="MPKIRDIRCRAFDVPLREAFGIAGGTQFTAQNVLVELELDNDVV
                     GLGEAAPFPAVNGETQAAVLAALPAATAVLRGTEILSYRRVSDAIREVASGTPSARAA
                     LEIALFDAWARSASVSLLSFFGGAEARLRTDITIPTGSPEAAQDAARRAAQAGFSTLK
                     VKVGGPTASHDIAHDVARLQGICDAAPHAELVLDANASLDESSTCALFDGLAQLARRV
                     VLLEQPTAAEDVDTLRRLRSRLTVPIAADESARSAADVARLALHRAVDVINIKTMKTG
                     IVEALDMICTARAHGLGLMIGGMVETEISMSTSACLAAGVGGFQFVDLDTPCFMAARP
                     LEGGFRQRGSDLDLSQIELGHGVRHLLGPGADWSEGTERHA"
     gene            9435..>10532
                     /gene="ychF"
                     /locus_tag="VFQ61_01165"
     CDS             9435..>10532
                     /gene="ychF"
                     /locus_tag="VFQ61_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET9953077.1"
                     /translation="MGFSCGIVGLPNVGKSTLFNSLSNARAEAANFPFCTIEPNVGIV
                     PVPDPRIEALSKVVHPDREVPTTIRFVDIAGLTRGASKGEGLGNQFLSHIREVDAVAH
                     VVRCFADDNIVHVDGRIDPVADVATIQTELCLKDLETVSKRLDRARKQSKGGDPVEKL
                     AVTVCERLLTHLDGGAPARSFQCSDKESEITRELHLLTDKPMFYVANVDEGSLGNLDK
                     NAHFQALCAFAKQEGSPVVPICAALEAQISELEPADRPEFLKSAGLSEPGLNAVIRSG
                     YSLLGLITYLTAGKQEVRAWTIRKGWTAPQAAGVIHTDFERGFIKAEVIWWEDLVALG
                     SEAKCREAGKLSIEGKDYVVRDGDVMHFRFNV"
BASE COUNT         1859 a         3291 c         3364 g         2018 t
ORIGIN      
        1 acgatgccca cgtacatcca agctttggcc agcgtttgag ggctacactt gtcgtcacaa
       61 gccttgatcg tgccagtcag cacggcctcg gctttgtcga agtcggtagc cagatagtga
      121 gtgttgatcg cttcgtcgat cttccggttg gcggccgcgt cgcgcgtgta ctgcgcttgg
      181 gcagggaacg ccgtaaagac cacgaaggcg agcatcaatt ggcgaaggga cagtcggggc
      241 acttctcgaa tctccggggc tggtattggg cgccgagagt tcggtgcagg ttccgcttgc
      301 gggcgtcaag agggcaggcg ggctgttccg aacgggtcga cgaaacgaaa ctcatggggt
      361 gcgggtcttc gggcatccct ccttgacccc ctcggtccgg acgaattgca cgcgacgatt
      421 gagcgatcga ttttgttcgc tggtgttatc gacgaggggg cgctccatcc cgtaacccac
      481 agagctcaaa cgagcgggtg taatgccgcg cgcgacgagg tacttgcgca cggagcccgc
      541 gcgccggttg ctgaggtcac ggttgtaagc tggcgatccc acgttgtcgg tgtggccctg
      601 aacctcgact ttgattgcag agttcttgct gagcacttcc gcgaccgcgt cgaggatcgg
      661 gtaactttct cttcggatcc ggtcctgatt gaactcgaaa tgaatctgct gcgtgatctt
      721 cacttcgcaa tcggtcacta gcaccaaagc gggcttttgg gggcaaccga gcggctcgct
      781 ggtggtgctt cctatttcgt tcggacactg atctttgacg tccggaacgc tgtcgccgtc
      841 attgtccgga tcgggacaac cgtctgcatc ctcgtacccg tcagggtctt ccgctgcgac
      901 tgggcatttg tcatcagggt ctagcgcgcc gtccacgtcg ttatcgagat ccgggcatcc
      961 gtcatcatcg agataggaat cgcggtcttc ggcttcgaga tcgcaagcat cgcggctatc
     1021 ggacacgccg tcgccgtcgg tgtccgggtc atctgggcag ccgtcggcat cttcgaagtt
     1081 gttcaaattt tctggctgct cggggcaacg atcacgatcg tctggaaagc cgtcgccgtc
     1141 gcggtcgccc gcaatttgta cgtatgcgcg cgtggcgcag tattcgggcg gcgaaagcgc
     1201 gagcgccgcg cgggcctggg gttccgctcg aaccaagtgc gcttgcgcgc gcaaggggaa
     1261 gccttggtcc agctccaggc tcgcgaactt gagttggctg cgggcgagcg cgagctcgat
     1321 gggggcgcaa cgaacggcgc cgtttcgttc cgcctgctcc actacttttt cgaggcctgc
     1381 gacgcgcccc cgcagccgcg gcgcctccgc gcaagagaac accagcagcg agagccctgc
     1441 gcaagcaacg ccaacgcatc tgctgaagga gacgcagcgt ttcgctcgcg cgatgcctgg
     1501 cgcgctgctc agtccgttag atgccagtgg gtgcttcggc ggagccccgg caattggagc
     1561 aaccggcgca tggcgaaccg gagcaccgag gaaccggcgt gcgctcatcg gccagcacct
     1621 cgatgggcgt cgcgagcgag tcggctcgct ttttctgcgt actcggcggc cgcgtccgaa
     1681 aggtcgagcg cgtccccgta ttcagcctcg gcggcttcac tacgcgcttt ggccaggtgc
     1741 tccttggcca tgtagaactc gtacggagcc aggtgctccg cgtctagttc ccgcgcttcc
     1801 tcgagctttg ccgcggcttg gttgatgctc agcgcgtaca gcccatggcc acagcccagt
     1861 agggggctca cgctgagccc gagccaggcg agtcgccaaa accaaagggc cggatgggca
     1921 gagcgggtcg acccagacag cgcggcgatt ggggacgcat ccgtgcgatc gcgagtgctg
     1981 ccgtcgatgt tcctggatgc tcgcggtttg ccgtcacttc gatccgcgat gtgtgtgact
     2041 gcgctggcgc tctggccagc ggcgcggcgg gtcgtttctg aaaccgtgct gggcctagct
     2101 tcgcgttgca caggccactc gcgataaaag gccgcctgca cgacacgcat gcggaaaacg
     2161 acagcgtgac agcatgattt ccgcgaaacc taccaatcac gcaggtttcg cgtcaacgcc
     2221 catttcactc tgcccggaag cccggactgc gctgtagcag gatctttgga aggagcttgg
     2281 cacaggcctg tgcccccgct tcccgactcg ctaagcgcga tgcaaatgcg gccttaccgg
     2341 agagcgctga aatcagcgct ttcatggctc ccgcttcgcg attcgctaac tagcgatgct
     2401 agcggccgac tggcgatgcc tagccgacgc gcaggtgcaa ctcgggggag cttcggcccg
     2461 gctaactcag agacttcggt aaaccccgat caccttgccg agcaacatag tcggcttgaa
     2521 gtcagttgct cgaaccagaa tgggtgccat ctgggcgtta gcaggctgga accgcacgta
     2581 gtcgcgctcc gggaaatagt atttcacggt cgcctcatcg ccgatcaaag ccaccacgat
     2641 gtccccgcgg tctgcctgcc cctggcgctt cacgaagatg tagtcgccgc tgaatattcc
     2701 ggcgtcgatc atggaatcgc cggttacctt gagaccgaag acctcgcgcc cgccgcgcac
     2761 cagcatccgg tcgatgcgca gcgtgtcgat cacgttctct tcggcgaata gcggcaagcc
     2821 tgcggcgacg cgcccgagaa cccggacctc gatcaagtcc tcttcgtcag tccgtgccga
     2881 cgctgggggg ctgtcctcga cgagcttcag ggcacggctc ttcatgtcct cgcgccgcag
     2941 gtagccctta cgttctagcg cccgcaaatg gtcgttcacc ccgttggtgg aacgaatgcc
     3001 catgtactca ccaatctcgc gaagggtcgg ggggtacccg cgctcttcaa tcgatttgcg
     3061 gatgaaatcg agcgtctgct cctggcgctt cgtcaaaccc tgcatggcgc cgcgaaggat
     3121 actgaacggg tgttaagacg tcaaattctg cacagccgtg tcgggtgatt agccaaaaac
     3181 atgcgcagtt tcgcaggctt agttgagcga acggaggcgt ggagaacgcc cactctccag
     3241 ggtgaacacc gcaattttct cgtcaaaagc gagataggtc gaatcgaaaa aattcgacga
     3301 gtcgtcgcca cccgatcgga tgccccggcc ccacgctgcg cttcagcccg gcacgagatc
     3361 gcttggcgcg gccccggaag ggtagcccaa gggataccgt cagaatcggc tgctgatgga
     3421 gagaaaggtc aatttgtcgg tagtgtttcg cagcctaccc accagctgtt tgactacgtt
     3481 ccacagcacc tcgtaagcga ggtccttatg caaaaacagc aagtcatcga aggcgtcctt
     3541 cgagagcacg agcaaccggc attcttcgtg gacgtgcgcg tccgccgacc gcggagcctc
     3601 gtccagtagc gccatctctc cgaaggcttc accgggtccc agcaccgcaa gcgcttcctc
     3661 gcccatcccg gcgacgtccc ggctgatccg cacctttccc tgcaggatca ggtacagctt
     3721 gtcgcccgcc tcgccgtaac gaaagacctt cgtgtcgaca cgatggatct cctcgttcgc
     3781 cacttgggcg attagctcca gagcctgagg tttcaggcct gcgaacaaat cgactcgggc
     3841 aagttgctcg actcgctccg cgcgttccgg ttggctcagc atggttgacg ttccggcgcc
     3901 cttcagcgcg taaaacgaat ccagaatacc gcagtttgcg tcacgggctg cccacttcga
     3961 tcgagccccg cgataaaggg cttgcgcagc acgcaatccc gcgtgacccg cccaaaaccg
     4021 tgacccggat cgtccaagac cgttactgat ttgggtctcg aatccgggcc gacgaccacc
     4081 gacacgcgaa cgcgagcctc attgatgcct tcgatgtccg cgtccggagg aaatggacaa
     4141 tcccgccaat tcgcgtttcg atctggcgcg gccggtcgtg agcgatcctc cgcaggtggg
     4201 ggaggcgcaa gcttcactcc cgtaccaccc ggcacgccgc caggcgtggc gttggtatcc
     4261 cgcacggcga ccttcgaggt gcctcgggtc gcggtgatgc cgcccgcata gcggtccgcc
     4321 gtcccggtca cgaatccttc acccgtcagg tccaccggtg cgttcggatc gggatcttgg
     4381 gtgaggacct tgccggcctc ggcgggcgcc ggtgggggag gcggcgcctc ggccgcagcc
     4441 ttgggaggcg gcgcggcctt ggcgacgggt ggtggttcgg gctcgggctt aggaggttct
     4501 ggctccggag gaggtggcgg tggtggcggc ttttccgctt ccacctccag ttcggagccg
     4561 agccgatgcc ggacgatact gatcacgtct tgagtgaacg tgcgcagatc gttgaaagaa
     4621 cggaaggctt gggagcccgg cgccccgtgc aacgcgagcg ccccgagcag cccgatcacg
     4681 gccccaatgc gcgcctgttt tcgcccgaga gcgatgatcg gcgccaacgg atccccgagc
     4741 tgcttgcgcg cagcgagctc ggtcgggcct gagcccttga tgctcgtgag cgcgacggga
     4801 ttccgagacc gcacgggttg cgtcatgagc cgttacttcg tgggcgccgt gtcgaccggc
     4861 gtgccttcag ccaacggaga taccgcgaag gcgatcttgg caatgccagc gcgcttgagc
     4921 aggtccagca catgaattac gcggccgtgc tctaccttgc gatccgcgcg gatcacggcg
     4981 cgcagctctt tgttcttgtt cttggagtcg cgagccaacg ggaagatggc ttcatcgttc
     5041 gggattaccg cggagtctac ccgcgtcttt ccgtctgcgc tcagctccac cgaaaacacc
     5101 gtttggagtg attcgccgct ggccgccttg ggcaagtcca ttggcatgcc ttgcgaaacg
     5161 atgatcttcg cggtcaccat gaagatgatg agcaacacca gcgtaacgtc caccagaggc
     5221 gtcacgttga tgccggagat cagcccgtcg tcgtcgtctg ccgcgccacc cgccacgagt
     5281 cagttctcct cttcggcgtc cgcggcgttg attttggccg cgcgtactcg ctcggctttg
     5341 tcgagcttgc tggcgctgga gcgcaacggc tccgtcgggt tcgcgaggta agacagcagc
     5401 acgtggctca gcgcggccgt gttggccagc acggctttgg agaggcgctg atagtagttg
     5461 aacatcacga ccgccgggat cgcgaccgcg agacctacag ccgtggccac gagcgcttct
     5521 gcgatcgctg ccatcacctc ttgcggtgcg gtagcggccg cggcggccgc cgccttcgct
     5581 gcgggcccgg agccctgctt tccgagctgt tcgaatgcct ccaccacgcc gattaccgtg
     5641 ccgaacaagc cgatgaacgt cgcgttgttg ccgagcgtcg ccaagaacgc caagcgccgc
     5701 tcgagcttga tcctctggag ggccatcgcc ccttgcatgg cctcgtcggc ggcctttgct
     5761 ccgcgatcgg cttcggccaa gcccgcgacc accaccgcgg cttcggcgct cggtgagcgc
     5821 tcgagtgaag cacgtgcccc ttccaggtcg ccgttacgca gctgatcccg gagcttctgc
     5881 gctagcgcgc cgatgtcgtc tcggagtgac cagtaaaacc aaccccgctc gagcatgatc
     5941 gcgaccgaca cgacgctgag cagaatcaac agccacatga cccaccccgc accgaagttg
     6001 gtcatcagct tttgtagcca cggcactaga tccatcggaa ctttcctcgc tcctcttaca
     6061 tgctgaaatg gtgagatgtg tcggtcgctg agactgcagg gcggcgggtc cttgcgagct
     6121 cggatggagc cagaccgggc acggcgagag tcgacataga gccaaagctc aacttcggaa
     6181 gtggctggtg cattccgtat gcccacgcac ctgagagacg agcttggctt cgcacgacac
     6241 cgaggcggcg tgattagcac aaaaaactgg cgcggcgccc tagccaggct gtctcgtctt
     6301 gtcggtcgcg tggccggtgc tcgtggcgac ggggtgatga gatgaagtgc cgttcatgag
     6361 cagctcggac ccgtgaacgg caattcacgg ctccaacagc ggcggctcgg cggcggggcg
     6421 gacccgttta gcccctgcct gttgcccctg cctggccagg tttggcgcgc cttcagccga
     6481 aatcgctcac aactcgtgcg gcatcaagtc gtagggggag cggaagaact gctgcggaga
     6541 gtacgccttt aggcaagaga cgcggcgcat gtagatgtcg gcgtacaggt cgacttggag
     6601 gccgaagctc gacatctcgt tttgctcttt gagcaacgag ccaaaataag ggtgaaagcg
     6661 cgtggagatg cgctcggcca gggtctcgtg ttccgagagg ctagaacgga gctcggttcg
     6721 aatacgatcc agcgctcgtt tcacccgcgc gcgctcgctc tcaccgaact ccgctgggtt
     6781 gcgcatcagc tccttgtagc gcgtctgata atagcggagc tcatcttcga gccggtcgcg
     6841 cgtctcctcg aggacccgtt gccgcgcgag gtcctcggca cagacttcgt aggcctccac
     6901 ctccgcgtcc agctcgggaa tgatcatcgc ggtgtgccag gacgattcct tcttggatcg
     6961 caggatgtcg ccgtagatgt gatccccgac atacagcacg ctcgagcctt ggacggacgc
     7021 gagacgctcg aaatcagcga ggtttccgcc ctcgtagacg cgtccacgct ccagctgacc
     7081 gcgcacgtgg cgcaggtttt ccccgtcgcg ctccagcaac gggcgccctt cttggaacca
     7141 acgcggcttc gccgcggcgc agatgacgat atcgaagaag tgacgccagg tcgggtactc
     7201 cggcatcgcc tctccgagca ggtagttcat catcgagtgc gtgtaatgaa agggcgagtt
     7261 ggtgagcagg aataaatgct tgcccgccga gcgaagctta tggagcgtac gagcgagctc
     7321 gggatcccgc tcgatgtagc gcgggaagtc tgcggtgatt gccgcgtaga cggcgccgtc
     7381 cgcgtgagcc tcgtcgatcg ccgagcggac gtcgctgaac agtcgatcga agtccacctt
     7441 ctcgccgcgc ttctccagcg cgtccacgat cgccgcgtag ctggtcacct ccgacagcgc
     7501 aaatagcgtg tcgatccagt gatagcgcgg agtgtgagga cgaatccgct tgtgatggta
     7561 gagctccgcg agctcctcgc gcggcaagcg gcgcataccg tggtagccgt agtgcaccac
     7621 cttgtagcgg tccatcttca ggacatgacc gtgccgcttg tccacgagca ggcctcgaat
     7681 ggggaagcgc gtgtcgtatt cgaggtgctt gaggtagctc gggtaaccgc gccggatcag
     7741 ccgttcgacc gtcagctcca cgctcaaccg atccatctcg tcctggcggt agatggcgag
     7801 cgtgtagtcc atgtcgaacc cgaaccactg aatccccgcc aggcgcaggt cacgattcac
     7861 gaacacgcga tcttttcgac agattgcgtg accgttggga ggcggtaggc tgactcccgc
     7921 gatggggagg atgagctgtc gaggatccga gaccgggctc ggaatagcgg tcactggtat
     7981 gtcacccacg agcaaaccta caaactagca gggtttctta gaagcgctct catggccggg
     8041 tttcccgctc ctattccctt tgtgtgcctg gctttggtgt tttggagcgt tctcgagatt
     8101 cgagcgttcg ggattcacgc atggcgttct gttccctcgc tccaatccgc tcctggccct
     8161 agtagatgac gcactccgtg ccctagctcg atctggctca ggtcgagatc tgacccgcgt
     8221 tgacgaaagc ctccttcgag cggccgagcg gccatgaagc atggtgtgtc caggtcgacg
     8281 aattggaatc cgcccactcc tgccgcaagg caagcggagg tgctcatcga gatctccgtt
     8341 tcgaccatgc ccccaatcat tagaccgaga ccgtgggctc gcgcagtaca aatcatgtcc
     8401 agtgcctcca cgatgcccgt cttcatggtc ttaatgttga tgacatcgac ggctcgatgc
     8461 agcgcgaggc gagcgacgtc tgctgcgctg cgcgcgctct cgtcggcggc aatgggcacg
     8521 gtgaggcgcg agcgaagccg ccgaagtgtg tccacgtcct cggcggcggt gggttgctcg
     8581 agcaagacga cgcgacgggc gagctgggcc aggccatcga acagcgcgca ggtgctcgat
     8641 tcgtctaggc tggcgttggc gtccaggacg agctccgcgt gcggtgctgc gtcacagatt
     8701 ccctggaggc gcgccacgtc gtgagcgatg tcgtgactcg cggtggggcc gcccactttg
     8761 acctttaggg tcgaaaaccc agcttgcgcg gcccgtcgcg cagcgtcctg cgccgcctcg
     8821 gggctgccag tggggatggt gatgtccgtg cgaagtcgag cctctgctcc tccgaaaaag
     8881 ctgagaagag acacgctggc gctcctcgcc caagcgtcga acagcgcaat ctcgagcgcg
     8941 gcccgcgcgc tcggcgttcc gctcgccacc tcacgaatgg cgtcgctgac gcggcggtaa
     9001 gagagaattt cggtgccgcg taggaccgcc gtggcggcgg gcagggctgc caacaccgcg
     9061 gcctgagtct ccccgttcac ggccgggaag ggcgcggcct cgccgagacc caccacgtca
     9121 ttgtcgagct ccagctcgac cagcacgttt tgcgccgtga actgggtgcc gccagcgatt
     9181 ccaaaggcct cccggagagg gacgtcgaac gcgcggcagc gaatgtctcg gattttcggc
     9241 atggttccat tccggaggcg gggttgacct caggtatcga gctaaatcag gccgtgacgt
     9301 gtcgcgcaag agccatcata cgcggccgat tcagggcggc ggttttgcgg agcgctgatg
     9361 gccgcggcgg atgcaatgcg aattggtgga agcgagttgc ggcgccgcac ggaggacgct
     9421 agaccccgcg tcccatgggc ttttcctgcg gcatcgttgg tctcccgaac gtcggtaaga
     9481 gtacgctgtt caactcgctg tcgaacgcgc gcgccgaagc ggccaatttc ccgttctgca
     9541 cgatcgaacc gaacgtcggg atcgtgccgg tacccgatcc gcgcatcgag gcgctgtcga
     9601 aggtggtcca ccccgaccgc gaggtcccca ccacgatccg tttcgtggac atcgcggggc
     9661 tgacgcgcgg agcctcgaaa ggtgagggac tcggcaatca gttcctttca cacattcgtg
     9721 aggtcgacgc ggtggcgcac gtggtgcgct gcttcgctga cgacaacatc gtgcacgtgg
     9781 atgggcgcat cgatccggtg gcggacgtcg cgaccattca gaccgagctg tgcctgaagg
     9841 acttggagac cgtgagcaaa cgcctggacc gcgcgcgcaa gcagagcaag ggcggcgatc
     9901 ccgtagagaa gctcgcggtt acggtgtgcg agcgcttact cactcatctg gatgggggcg
     9961 cccccgcgcg ttctttccag tgctcggaca aggagagcga gatcacgcgc gagctgcacc
    10021 tgctcaccga caagccgatg ttctacgtgg ccaacgtcga tgagggatcg ctcggcaacc
    10081 tggacaagaa cgctcacttt caggcgctct gcgcgttcgc gaagcaggag ggcagcccgg
    10141 tggtgccgat ctgcgcggcg ctggaggctc agatctcgga gctcgaaccg gcagatcgtc
    10201 ccgagttcct gaagagtgcc ggtttgagcg agcccggact gaatgccgtg atccgctcgg
    10261 gctattcact gctcgggctc atcacgtacc tgaccgcggg caagcaagag gtgcgcgcgt
    10321 ggacgatccg caagggttgg accgctccgc aagcggcggg cgtgattcac accgatttcg
    10381 agcgcggctt catcaaggcg gaagtgatct ggtgggaaga tttggtcgcg ctgggcagcg
    10441 aggccaagtg ccgggaagcc ggcaagctga gcatcgaggg taaggattac gtggtgcgcg
    10501 acggcgacgt catgcacttc cgcttcaacg tc
//
LOCUS       DASTKU010000036        16410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_452919, whole genome shotgun sequence.
ACCESSION   DASTKU010000036 DASTKU010000000
VERSION     DASTKU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16410
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452919"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VFQ61_01170"
     CDS             complement(<1..1339)
                     /locus_tag="VFQ61_01170"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HET9953078.1"
                     /translation="MMRRIGPSSPLTRATRRGLVRRRRARRRGMAIILVLGALTLLTI
                     LLTEIQDESSADLAGALQARDALVAEYAARSAVNLSRLLIASEPTLRKGLGFMALMFG
                     GAIPQIPVWQLGDTVLAPFNNPAAAESFQSFSGLDLTRGQNLGLPGATFDLKIVDEDS
                     KINVNAAANPFVQIRLSNMLMALMAGPQYDPLFERRDRDGNFSDRRAICAALCDWVDS
                     ADATTALCDPTSQTNQQMPAEDSYYERLDRPYQRRNAPFDSLEEMRRVRGMGDDFWST
                     FIDPDPDDPSKRRVTVWGSADKININTAPPASLLVLICYMASVSNAAVIPKVCSDLTE
                     AQKFLTMVGFAKSMMAGIPAFGSPGVFAAALQGNAQEPLKSLIMMLGLEPIPLDPGLL
                     KQLVTTESKVFSVYVTGVVKSGKRETRVKVHAVVDFRAAPPPGMPTVPPQLAAA"
     gene            complement(2045..2878)
                     /locus_tag="VFQ61_01175"
     CDS             complement(2045..2878)
                     /locus_tag="VFQ61_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9953079.1"
                     /translation="MRPPRSQRGFTLLEVLVAIAILGLGLTMILSSQVGLFSSATRAQ
                     RLTIATNLARCKMSEVEVDLLKLGYPLTDTKYEGRCCGDDSDPDYDCAWKVERVKLPE
                     GDNTDGGIDAALDPMAKLASITSKPASSGSSSSSPGLGGLAPLGSALPGLGGLGAPGA
                     LGGGSDMLSGLMGAATGPGGGLGGMLMSMAYPSLKPMLEASIRKVTVRVHWKEGKVDR
                     EFSVTQYVTDPQQGGLEADAGLLDAGITGLPGMPGATGLPGMTGQQGIPGLSGLTGLG
                     R"
     gene            complement(2875..3579)
                     /locus_tag="VFQ61_01180"
     CDS             complement(2875..3579)
                     /locus_tag="VFQ61_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9953080.1"
                     /translation="MRCRASARGLTLVEVLIVVALIALLGGTAMLGPGLLRSTRLRTT
                     ATAIVSGVRLGLTRANTTGRAVRLVFDLDERRLSMEEAQTSEFTRKKDVTGGADASTE
                     AEKKAKEEAERTVSGPRAPALRFKPISVLTDPEDSKKGRPFGAGVEIASVQTEHDEEP
                     VTQGRAYLYFWPGGMTERATIRLKRSDGTLDEGLSVVVSALNGRASIVKGEPKLAQPP
                     EDPEGSGFGNPKEEEP"
     gene            complement(3589..4056)
                     /locus_tag="VFQ61_01185"
     CDS             complement(3589..4056)
                     /locus_tag="VFQ61_01185"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET9953081.1"
                     /translation="MQSNWSSWMAESWMKVLRAQRRAARRRVARGVTLFEVLIVVAIL
                     AMVAGGVASFALPKFKQAQISQAEVGARTMRTAIQQWQASNNESSCPTVSQLVQEKQI
                     DPGQSTNDPWNQAYTFNCSDDEVTVISAGPDKKKGTKDDISIPKGATAPGEGN"
     gene            complement(4066..4476)
                     /locus_tag="VFQ61_01190"
     CDS             complement(4066..4476)
                     /locus_tag="VFQ61_01190"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HET9953082.1"
                     /translation="MPREGSSSAIFFPWERRRGVRRWLGTGRIRPVLSVVFVLGFVII
                     VGARERQKSGVRQTRATLLAMRHGVDAYIADNEGKCPESLQQVLSHTSYTQVPSDAWG
                     NSLRLTCPARREGERYELMSDGPDGKPGGLDRIE"
     gene            complement(4479..5708)
                     /gene="gspF"
                     /locus_tag="VFQ61_01195"
     CDS             complement(4479..5708)
                     /gene="gspF"
                     /locus_tag="VFQ61_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HET9953083.1"
                     /translation="MAVFEYRGIQIESGKAVKGYRDAENARALRAVLRRDGIMLTSAS
                     EESEQKRQSKQRNIDLLAFFKRPRAADVAVMTRQLATLVRAGVPLVESIAALTDQVEN
                     EQLVRVLTTVRESLNEGTSFAKSLEQHPSVFPPLYVNMIAAGEASGTLEAVLERLADF
                     MEGQAKLRGKVMSALAYPVLMAVVGSLMVGVLMVGVVPKVTSVFDNLGQTLPWYTRLL
                     IFVSDTIAGFWWLILGSLGLGIYAFQRWKRTPKGLLKWDAMRLRMPLFGRLTLLVAVA
                     RFSRTLATLLSSGVQLLPAMEIGRHVLENARLSTVVTEAIGSIRGGESIAEPLKRSGA
                     FPPMVTHMIAIGERSGQLEAMLENVSRAYETDVETRVSALTSLLEPLMIVLLGGVVGF
                     IALAILMPLIQMNQLVE"
     gene            complement(5720..7489)
                     /gene="gspE"
                     /locus_tag="VFQ61_01200"
     CDS             complement(5720..7489)
                     /gene="gspE"
                     /locus_tag="VFQ61_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HET9953084.1"
                     /translation="MLQDSLLGSILTRRGLATPESLRGLLIEPGDKKTLLETVVEAKL
                     VTELDLARALAEECGVPFQEQVRTDEIVTEAAIKLPISYAKAHRLIVSEEHPDYVQVI
                     CADPLDTQAIDDVRAAFEKPVVLHVATGESVMDAINRVYERQANLSELEAKDELEAEG
                     QVDILDSDDDAPIIRWVNGLFSQSVKERASDIHIEPEEREVIVRYRVDGQLYVARRAS
                     RQFMSSVIARIKIMAGLNIAEKRLPQDGRISLKIAGRSIDVRVSTIPTSRDHERIVMR
                     LLHKTNVLLDLRDIGFSERDYALMTQLIERPDGIILVTGPTGSGKTTTLYACLNRINR
                     PDINILTAEDPVEYEIGGIHQVAVQAKIGLTFASAMRAFLRQDPDVIMVGEIRDRETA
                     EIANHASMTGHLVLSTLHTNDAASAFTRLVEMEVEPFLVRSTVIGILAQRLVRVLCQN
                     CKQPYEATPYELQQLGIDPERTRRRDERVLSPRYQPYGVTYEPIGWRGPGMPTFYKPG
                     GCDRCDNKGFVGRLGIYELLVAEDAVGRLVLSNADAQSIKRAAQSQGMDTLRDDGARK
                     VLLGKTTVEEVVAATQDDVLVDE"
     gene            complement(7492..10083)
                     /gene="gspD"
                     /locus_tag="VFQ61_01205"
     CDS             complement(7492..10083)
                     /gene="gspD"
                     /locus_tag="VFQ61_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HET9953085.1"
                     /translation="MRRFRLLLSSCLILSVATPALAQDAAPAAPANDAVLRRRLRNLA
                     RKPKAAAAAEPAAVTPPPAAAAVAAAPAPEKEPPGAAGPLPSNMDKIAAAADVPYKPK
                     PGGHLVKFNLQDADLAELVNHISGLTGRRFIYGAKVRQVKATVVSPEPVTLEEAYQAF
                     LSILEANGMTVVPHGRFLKIVDSGGVVGVATPLYSRGEPLPDADRYITRLYRLENVTP
                     DDVVPILSKFKSKEADISAYNPGRLLIITDTASQVQRLIRLIEEIDVGGTGQHMWIEP
                     VRNGQAQEMSKRLNELFELGGAPQPGQPAGGPRAHGLSRVIADEASNSLIVVGTQDGY
                     LRLLELLKRLDAGKTETGRVHVLPLQHGIAEEMANTLTQMLGGGGKNAQGANANGMFE
                     GDVRVTADKSTNSLLITSSNRDYATLRLVIDKLDRARRQVFIEAVIMDLAVSDQMTLG
                     VAFHGGKVLGEGGESGSSSVLLGGFRSGQSIGFPTDPSLLQGLAAGIRGPNLPDTTRL
                     LGTGLSVPAFGVVVNALASSGKSNVLATPHVIATDNVAAEISIGDNIPLQTNLGGGNL
                     QNLAGAAGQTGAAGLGLLGGLGFGGFSAPRQDVGNKIKVTPHVNESNQVRLEIDQESS
                     SPGAAVGALGAIPIQKRTANTTVVVQDQQTVVIGGLMKDEYVTSREKIPVLGDLPLLG
                     ALFRRNETTKRKANLLLILTPHVIRDQTDLRRIFERKMQERQEFLDRYFVFSGQEWTP
                     PRDWSRTNGLVEQIRKSYQELEEQARLQLESEKITIDREPSDPIELPSDVRAGEEPAA
                     PAPARRGRNAAPAANPGQPAQAPAPRPGRPQIPRVAPPAPAPAPPQGANPSPFKTAPL
                     ARSVDTE"
     gene            complement(10095..11075)
                     /gene="gspC"
                     /locus_tag="VFQ61_01210"
     CDS             complement(10095..11075)
                     /gene="gspC"
                     /locus_tag="VFQ61_01210"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HET9953086.1"
                     /translation="MALDALIKKYFTVVILAMVAMSAYFEAAGATQLLGAALLAAPSG
                     SAPHAVPAPPIVIPSRERSTADAIIDRNPFDSVTGPLHPKPEPEALTPKPDTIANTDP
                     LTAPQCDGVSALIITESTDRLWSVAALQATGETAPKMRRVGDDVGGKKIEFIGFNPRE
                     SSPAVWCSSGGQLCQVLLFKSAAQPTPTAAPVAAAPPPPPPPPAGGAVPADIASRIQK
                     VSDSEFNVDRQVVDKILENQAELMKSARIVPEQKDGKVVGIRLFGVRPDTLLGTLGLQ
                     NGDRLETINGFNMASPEKALEAYARLRAASELNIKVNRRGSPVSIDYHIK"
     gene            complement(11320..13215)
                     /gene="htpG"
                     /locus_tag="VFQ61_01215"
     CDS             complement(11320..13215)
                     /gene="htpG"
                     /locus_tag="VFQ61_01215"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HET9953087.1"
                     /translation="METVRHPFQAEVSQVLNLVIHSLYSNKEIFLRELVSNASDALDK
                     RRFAALKDPSLLEEGEVLKIRLSADLDAGTLTIWDNGIGMSEAELAKNLGTIAWSGSR
                     EFINRMKGEGAPAQDLPTLIGQFGVGFYSAYLVADRVTVTSRAAGSAQAYSWESTGEE
                     GFSISEAQRDSVGTSIVLHLKAEQREYLEPYRLRELVLRYSDYVQHPIELVGSNDPKK
                     EAEVETINRGSALWQRSPKEVTHEQYVEFYKHQSHDFEPPLGYRHFQIEGTQMFTALL
                     FVPGRAPFDLFDPEASHGVRLHVRRVFVMDNCQELLPRWLRFVRGVVDSEDLPLNVSR
                     ETLQDSRAVKAIKKQIVAKTLELLGEIAEQRPDDYDKFWSTFGVVLKEGLYFEPEQKD
                     KLAKLLRFESHAQGKSISLDAYVANMKSDQPAIYYVLGPNQKVASSSPHLESARSRGY
                     DVLLLTDPVDSFAISALEKYGDKPLVSVTGADWKLPEGEQAGEKKPDEEANASVLKGF
                     LEVLKDRVLDVKASSRLTDSPACLVQAEGGLPPHLERLFKAQKRDLPASRRILELNLN
                     HPLVKSVTALNARESSSPRVKEWLELIYDQALLAEGSPIEDPAAFAKKLTRLFSEAAA
                     HELGQPS"
     gene            complement(13404..14174)
                     /locus_tag="VFQ61_01220"
     CDS             complement(13404..14174)
                     /locus_tag="VFQ61_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953088.1"
                     /translation="MRTVSCLALLALLAGCGGSELDLGTYVPPSRGGSDGFEGLAGRA
                     SGGQLAGGASSVAGSPSGGGASPVGGASPVGGVSPVGGASPVAGAGNIGGVTPVGGAV
                     GEAGAGGAEPVGGTSGNGSLRSLTLVTVSGFPPPPSPEDECDLTKFVTIHAVNFEESS
                     LSTSTCDGFPTRFESDARQLTELELTELRQAIEQIAPSSNTECTVLDKGPLLLDLERA
                     GSVTKYQDDANACNPPRDGRTYATSLDNLRSLLVRLSG"
     gene            complement(14293..15303)
                     /locus_tag="VFQ61_01225"
     CDS             complement(14293..15303)
                     /locus_tag="VFQ61_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953089.1"
                     /translation="MTDPKRLPDTDLDEFDRVLLNSAKGDVGSERALVRSLAAFSAAS
                     LMTPSATAALSKAAWFSALKFLGAGVLVGGLVVGAARPEWRTALGWGHGLDRAASGVR
                     PSRAAGASPLASSTRATPRVAVVEGQLFTERTGGESTPIGIGEPPKPVEARAAASRTV
                     APQSDQVSPQRTITDRSEPAEKVPSLLDRTLLLDRTSRGEPHSVSDRTTATPDGAPLQ
                     GSAAFPEPPGDASAGELRTRRAADSTSDELIREVAVLDRARAALAQQRGARALHILDL
                     YDREFPRGTLGPESIVIRVEALESVGAKEQARHLAEKGLRELPEGAHSARLRAWLRRS
                     NP"
     gene            complement(15300..15938)
                     /locus_tag="VFQ61_01230"
     CDS             complement(15300..15938)
                     /locus_tag="VFQ61_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9953090.1"
                     /translation="MLVSKVLLSRNPFSKLIRHVPPSSEPVRAGAHARLEAAARENFQ
                     FIWRCLRRFGVRPEHAVDDAAQRVFEVAARKCEQIEVGRERAFLFKTAVWVAAEVRRA
                     QRRDEYVDLDELHGEHLVAASPAPEQAAIDRELRHCLDEVLGAMPDELRTVFVLFELE
                     HLPAGDIAELVGIPVGTVASRLRRARQDFHARARRWRAKFEFFAGGARENQR"
     gene            complement(15964..>16410)
                     /locus_tag="VFQ61_01235"
     CDS             complement(15964..>16410)
                     /locus_tag="VFQ61_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953091.1"
                     /translation="EHGPVLGLALSGARVSSGTLAQPYGAAELTWMTLRAEFCSAASW
                     PRARFFLVGGCAFLDLGQLAGQGRATGSPDAVQRASLWVAPGIHALVAWEWRRVLRLS
                     AGIGVHWPLKRPEFRFSERDSPVTHDIYVVPNRPAVSLDWGGALLF"
BASE COUNT         2945 a         5483 c         5000 g         2982 t
ORIGIN      
        1 aagccgccgc gagctgcggc ggaacggtag gcatgccggg cggaggtgcg gcccggaaat
       61 cgacgacggc gtgcactttc acgcgcgtct cgcgtttgcc cgatttcacg acgcccgtta
      121 cgtataccga gaacaccttg ctctcggtcg tgacgagctg tttcaacaaa cccgggtcga
      181 gcggtattgg ctccaaaccc aacatcatga tcaggctctt cagcggttcc tgagcgttcc
      241 cctgcagggc ggccgcgaaa acgccgggcg agccgaacgc gggaatgccc gccatcatgc
      301 tcttcgcgaa gcccaccatg gtgaggaact tctgcgcttc ggttaagtcg ctgcaaacct
      361 tcggaatcac cgccgcgttg gatacgctcg ccatgtagca gatgagcacc aacagcgagg
      421 cgggtggcgc cgtgttgatg ttgatcttgt ccgcggagcc ccagaccgtg acccgacgtt
      481 tgctgggatc gtcgggatcc gggtcgatga acgtcgacca gaagtcatcg cccataccgc
      541 gcacgcggcg catctcttcc aagctgtcga acggcgcgtt gcgacgttga tagggccgat
      601 ccaatcgctc gtagtacgag tcctcggccg gcatctgttg gttggtttgc gaagtcggat
      661 cgcacagcgc ggtcgtggcg tccgctgaat ccacccagtc acatagcgcc gcacaaatcg
      721 ctcgccgatc ggaaaagttg ccgtcccgat cacgtcgctc gaacagcgga tcgtattgcg
      781 gccccgccat cagggccatc agcatgttac tcaagcgaat ctgcacgaac ggattggcgg
      841 ccgcgttgac gttgatcttc gaatcttcgt cgacgatctt gaggtcgaag gtggcgcctg
      901 gcagccccag gttctgcccg cgcgtcaaat ccagcccgga gaaggattgg aaggattcgg
      961 cggccgcagg gttgttgaac ggcgcgagca ccgtatcccc gagttgccaa accgggattt
     1021 gcgggatggc gcctccgaac atgagcgcca tgaaaccgag ccccttgcgc aaggtcggct
     1081 cggaggcgat cagcaaccgc gataggttca ccgcgctgcg cgcggcatac tcggccacca
     1141 gcgcatctcg cgcttgcagg gcgcccgcga gatcggcgga gctctcgtcc tggatctcgg
     1201 tgagcaggat ggtgagcagc gtcaacgcgc ccagcaccag gatgatcgcc ataccgcgcc
     1261 gccgcgcgcg acggcgccgc accaggccgc gtctcgtcgc gcgcgtgagc ggcgaactcg
     1321 gtccgatccg ccgcatcatg cgccgccaac gcgctcacgg gttgctggat tggtagcgag
     1381 atcgtggtga cgagcttgag ccggccgcgc ccgccttctc cgcgcgcgcg cgcgcccgcg
     1441 ttgagcacca aggtgacccg cacctgcaag ggtagacgat tgagctgcgc ggtcgtctgg
     1501 gtggtgtccc aagtctccag ccagttgccc gtctgcgggt cgaggtattc gagctcgaac
     1561 agatcgatgt cggaagcgag cacatccacc cggcccccgt gttgcggatc caaatccaag
     1621 aacgtgctct tgcgccgcag cagatcgagc gtgccttcgg cgccagtgcg ttccccgaag
     1681 taagagattt ccgcctgatc cgtctctttc gagtcacgat cgcgacgcac gttggagaac
     1741 gaattgaaat ccacgcgatc ggtggcggtg ccccggcgcc cgatgaagga cgtcttctgc
     1801 acgaggtagc gcgtatcgat cggtacgttc atcgagatat aggccgaggc caactcacgg
     1861 ctaatttgct gcatcgccag ccgcccctcg cgatagcgat tctcgactcg ctcgagccct
     1921 tcccgagtcc tcttcatgct cgagaaggcc ccgaacagca gcaccgagat catggccaag
     1981 atcgcgatcg cgatcagcag ctcgagcagc gtgaagccac gtcctaggcg gcggggtgcc
     2041 cttttcatcg acccagcccc gtcaaaccac tcagcccggg aatcccctgc tgcccggtca
     2101 tgcccggcag gccggttgcg cccggcatgc cgggtaggcc cgtaatgccg gcatccagga
     2161 gcccggcatc tgcctccagc ccgccttgtt gaggatcggt gacgtactgc gtgaccgaaa
     2221 actctcgatc gactttgcct tccttccagt gcacgcgcac ggtgacttta cggatgctgg
     2281 cttcgagcat gggcttgagt gatggataag ccatactcat cagcattccg cccaagcccc
     2341 cacccggccc cgtcgcagcg cccatcaagc cgctcagcat gtccgagccg ccgccgagtg
     2401 cgccgggcgc gcccaggcca cccaagcccg gcaatgcact gccgagcggc gcaaggccgc
     2461 ccaggcccgg cgaggaagaa ctgctacccg acgaagcggg cttgctcgta atcgaagcca
     2521 gcttagccat tggatcgagc gctgcgtcga tgccgccgtc ggtattgtcg ccctccggca
     2581 gcttcacccg ctccaccttc caagcgcagt cgtagtccgg atcgctgtca tcgccgcagc
     2641 agcgtccttc gtacttggtg tccgtgagtg gatagccgag cttcagcaga tccacctcga
     2701 cctcggacat cttgcaacgg gccaggttgg tcgcgatggt caggcgctgc gcgcgggttg
     2761 cgctcgagaa caaccccacc tgcgagctca agatcatggt caagccgagc cccaggatcg
     2821 caatcgcaac cagcacctcg agcagcgtga agccgcgttg cgagcggggt ggcctcacgg
     2881 ctcttcctcc ttgggattgc cgaagccgct gccttcggga tcttctggtg gctgggcaag
     2941 cttgggttcg cctttgacga tggaggcacg gccattcagc gcgctgacca ctacgctgag
     3001 cccttcgtcc aaggtgccgt cgctgcgttt cagtcgaatc gtggcgcgct cggtcatgcc
     3061 gccgggccag aagtagaggt aggcgcggcc ctgcgtgacg ggttcttcgt cgtgctcggt
     3121 ttgcaccgac gcgatctcga ctccggcccc gaatggacga cccttcttgg agtcttcggg
     3181 atccgtcagc acggagatcg gtttgaaccg gagcgcgggc gcgcgcggac ccgagaccgt
     3241 gcgttcggct tcttctttgg ccttcttttc ggcttcggtg ctggcgtctg cgccgcccgt
     3301 tacgtccttt tttcgggtga actcggaggt ctgtgcctcc tccatcgaga ggcggcgttc
     3361 atccaaatcg aataccaacc gcacggctct tcccgtggtg ttggcgcggg tgaggcccaa
     3421 acgaactccg ctcacgatcg cggtagccgt ggtgcgcagc cgggtggagc gcaaaagccc
     3481 gggtccgagc atggcggttc cgccgagcag cgcgatcagc gccaccacga tcaggacctc
     3541 gaccaaggtc aggccacggg cagaagcacg gcaccgcacc ggttctcctt agttgccttc
     3601 tccgggtgcg gttgcaccct tcgggatgga gatgtcgtcc ttggtgccct tcttcttgtc
     3661 cgggcccgcg gagatcaccg tcacctcgtc atccgaacag ttgaacgtgt aagcctggtt
     3721 ccaggggtcg ttcgtgctct gaccgggatc gatttgcttt tcctgtacga gctggctcac
     3781 cgtcgggcag ctcgactcgt tgttggacgc ttgccactgc tgaatcgcgg tccgcatcgt
     3841 gcgcgcgccg acctcggctt gcgagatctg agcctgcttg aacttgggaa gcgcgaagga
     3901 tgcgacgcca cccgcgacca tggccaagat cgccaccacg atcaaaacct cgaacagggt
     3961 cacaccgcgc gctacgcgcc ggcgtgcggc ccggcgctgc gctcgcagca ccttcatcca
     4021 agactcagcc atccaagaag accaattcga ttgcatgggt tcctctcatt cgatgcgatc
     4081 gagaccaccg ggcttgccgt ctgggccatc gctcatcaat tcgtaacgtt cgccctcgcg
     4141 tcgcgcgggg caggtcagcc gcaaggagtt accccaagcg tcgctcggga cctgcgtgta
     4201 actcgtgtga gacagcactt gttgtagaga ctcggggcat ttcccttcgt tgtccgcgat
     4261 gtacgcatcc acgccgtggc gcatggcgag cagcgtggcg cgcgtctggc gtaccccgga
     4321 tttctgccgc tcgcgcgcac ccacgatgat gacgaagccc agcacgaaca ccacgctcaa
     4381 aaccggccga atacgacccg tacccagcca acgccgcaca ccgcgccgac gttcccaggg
     4441 aaagaagatc gcactcgagg agccttcgcg cggcatgctc actccaccag ctgattcatt
     4501 tggatgagcg gcatcagaat cgcgagcgcg atgaagccaa cgacgccgcc cagcagcacg
     4561 atcatgagcg gctcgagcaa gctggtgagc gccgagacgc gtgtctcgac gtccgtctcg
     4621 taggcgcgac tcacgttctc cagcatggcc tccagctgac cgctgcgttc gccaatcgcg
     4681 atcatgtgcg tgaccatggg cgggaatgcg ccactacgct tgagcggctc cgcgatgctc
     4741 tcaccgccgc ggatcgagcc gatcgcttcg gtaaccaccg tcgagagccg cgcgttctcg
     4801 agcacgtgcc tgccaatctc catggccggc agcagctgca cgccgctcga gagcagcgtc
     4861 gccagggtgc gagagaagcg cgccaccgcc accagcagcg tcaagcgacc gaacagcggc
     4921 atgcgcaagc gcatggcgtc ccacttcaag agccccttcg gcgttcgctt ccatcgctgg
     4981 aaagcataga tgccgagccc gaggctaccc agaatcaacc accagaagcc tgcgatcgtg
     5041 tccgagacga agatcagcaa gcgcgtatac caaggcaagg tttgccccag attgtcgaac
     5101 acgctggtga ccttcggcac cacgccgacc atcaagacgc cgaccatcaa tgacccgacc
     5161 acggccatca gcaccgggta cgccagcgcg gacatcactt tgccgcgcag cttggcctgc
     5221 ccctccatga agtccgccaa acgctcgagc accgcttcca gcgtgcccga cgcctcgccg
     5281 gccgcgatca tgttgacgta cagcggaggg aacacgctcg ggtgttgctc caggctcttc
     5341 gcgaagctcg tgccttcgtt caagctctcg cgcacggtcg tgagcacccg cacgagctgc
     5401 tcgttttcca cctgatcggt caaggcggcg atcgactcca ccaacggcac cccggcccga
     5461 accagggtcg cgagctgccg ggtcatgacc gcgacgtccg ccgcgcgcgg gcgcttgaag
     5521 aacgccaaca agtcgatgtt gcgctgcttg ctttgacgct tttgttcgct ctcctcgctg
     5581 gccgaggtga gcatgatgcc gtcgcggcgc agcacggccc ggagcgcgcg tgcgttctcg
     5641 gcatcgcggt agcctttgac ggccttgccg gactcgattt gaatgccgcg atactcgaaa
     5701 acagccactc ggaattacct cactcgtcga cgagcacgtc gtcctgcgtc gccgctacga
     5761 cttcttccac cgtggttttg ccgagcaaca ccttgcgcgc gccgtcgtcg cgcaaagtgt
     5821 ccataccctg cgactgggcc gcgcgcttga tactctgcgc gtccgcgtta ctcagcacta
     5881 ggcgacccac cgcgtcttcg gccacgagca gctcgtagat gccgagccga cccacgaagc
     5941 ccttgttatc gcagcgatca cagccgccag gcttgtagaa cgtcggcatg ccgggccccc
     6001 gccaaccaat gggctcatat gtcacaccgt agggttgata acgaggcgag agcacccgct
     6061 cgtcccgacg gcgcgttcgc tccgggtcga tgccgagctg ctgcagctcg taaggggtgg
     6121 cctcgtaagg ctgcttgcag ttctgacaca gtacgcgcac gaggcgctgc gccaggatgc
     6181 cgatcacggt cgaacgcacc aagaatggct cgacttccat ttcgacgagg cgcgtgaacg
     6241 cggaggccgc gtcattggtg tgcagcgtgc tgagcaccaa gtgacccgtc atcgaagcat
     6301 ggttggcgat ctcggcggtt tcacggtcgc ggatctcacc gaccatgatt acgtcgggat
     6361 cctggcgcaa aaatgcgcgc atcgcgctcg cgaaggtcag gccgatcttg gcctgaaccg
     6421 cgacttggtg gatgccgccg atctcgtact cgaccggatc ctcggcggtc agaatattga
     6481 tgtcggggcg attgatccgg ttcaggcagg cgtagagcgt ggtcgtctta ccgctaccgg
     6541 taggcccggt caccaagatg atgccgtcgg ggcgctcgat cagctgcgtc atcagcgcgt
     6601 agtcgcgctc ggagaagccg atgtcccgca agtcgagcag cacgttggtc ttgtgcaaca
     6661 gacgcatcac gatgcgctcg tgatctcggc tcgtcgggat ggtcgagacg cggacgtcga
     6721 tgctgcgacc ggcgatcttc agcgaaatac gcccgtcctg cggcagccgc ttctcggcga
     6781 tgttcaggcc ggccatgatc ttgatacgag cgatcaccga gctcatgaac tggcggctcg
     6841 cgcggcgggc gacgtagagc tggccgtcca cgcggtagcg cacgatgacc tcgcgctcct
     6901 cgggttcgat gtgaatatcg ctggcccgct ccttcaccga ttgcgaaaac aagccgttca
     6961 cccagcggat gatcggcgcg tcgtcgtccg aatcgagaat gtcgacttga ccctcggcct
     7021 cgagctcgtc tttggcttcg agctcactca gattggcctg ccgctcgtag acgcggttga
     7081 tggcgtccat cacggactcg cccgtcgcta cgtgcagcac caccggcttt tcgaacgccg
     7141 cacgcacgtc gtcgatggcc tgggtatcca gcggatccgc gcaaatcacc tgcacgtaat
     7201 ccgggtgttc ctcggacacg atcaggcgat gtgccttggc gtaggaaatc gggagcttga
     7261 tcgccgcctc ggtgacgatc tcgtccgtac gcacctgctc ctggaaggga accccgcatt
     7321 cctcggccaa ggcccgggcc aggtccagct ccgtcacgag cttggcttcg accacggtct
     7381 cgagcagcgt cttcttgtcg ccaggttcga tcagaagccc gcgcagcgat tccggagtcg
     7441 cgagtccgcg tcgagtcaga atcgagccga gcagcgagtc ttgcagcatg ctcactccgt
     7501 atcgacgctc cgggccagcg gagcagtctt gaagggtgat gggtttgcgc cttgcggcgg
     7561 cgcgggggcc ggagccggcg gtgcgacgcg aggaatttgc ggccgcccgg ggcgcggcgc
     7621 cggtgcttga gccggctgcc ccgggttagc cgccggtgcg gcgttgcggc cgcggcgcgc
     7681 cggagcaggc gcggccggtt cctcgcccgc acgcacgtcg ctcggcagct cgatcggatc
     7741 actcggttcg cggtcgatcg tgatcttctc gctctccagc tgtagacgcg cttgctcctc
     7801 gagctcctga taagacttgc gaatctgttc gaccaagccg ttggtgcgcg accaatcccg
     7861 cggcggagtc cattcttggc cgctgaacac gaagtagcga tccaagaact cctgccgctc
     7921 ctgcatcttg cgctcgaaga tacggcgcag atcggtctgg tcgcggatca cgtggggggt
     7981 caggatcaaa agcaggttcg ccttgcgctt ggtggtctcg ttccgtcgga acaaggcgcc
     8041 gagcagcggc agatcgccca gcacggggat cttctcgcgc gaggtgacgt actcgtcctt
     8101 catcaggccg ccgatcacca ccgtctgctg gtcttgcacg accacggtgg tgttcgccgt
     8161 acgcttttgg atcgggatcg caccgagcgc gcccaccgcc gctccgggtg agctgctctc
     8221 ctgatcgatc tcgagacgca cctgattcga ttcgtttacg tgcggcgtga ctttgatctt
     8281 gttaccgacg tcctgacgtg gcgcgctgaa accgccaaag cccaagccgc cgagtaggcc
     8341 caagccggct gcgccggtct gacccgcagc gcccgccaag ttctgcaagt tgccgccgcc
     8401 gaggttggtc tggagcggaa tgttgtcacc gatgctgatt tcggcggcga cgttgtcggt
     8461 cgcgatcacg tgcggggtgg cgagcacgtt cgatttaccg ctgctcgcga gcgcgttcac
     8521 caccacgccg aaggcgggca cgctcaggcc ggtgccgagc aagcgcgtag tgtcgggcag
     8581 gttgggcccg cggatacccg ccgccaaccc ttggagcaag ctcggatcgg tggggaagcc
     8641 gatggattgc ccagagcgga agccacccag caacacggac gagctacccg attctccgcc
     8701 ttcgcccaac actttcccgc cgtggaacgc gacgcccagg gtcatctggt cgctcaccgc
     8761 gagatccatg atcacggctt cgatgaacac ctggcggcgc gcgcgatcca gcttgtcgat
     8821 caccaagcgc agcgtggcgt agtctcgatt cgacgacgtg atcaggagtg agttggtgct
     8881 cttgtccgcc gtcacgcgca catcgccctc gaacatgccg ttcgcgttgg caccttgcgc
     8941 gttcttgcca ccgccaccca acatctgagt gagcgtgttg gccatttcct cggcgatgcc
     9001 gtgctgaagt ggcagcacgt gtacccgccc cgtctcggtc ttacccgcgt ctagacgctt
     9061 caacaactcg agcagccgca ggtacccgtc ttgggtgccc accacgatta agctgttgct
     9121 cgcttcgtcc gcgatcacgc ggctcagacc atgtgcgcgc gggccacccg caggctgccc
     9181 gggctgcggc gctcccccga gttcgaacag ctcgttcagg cgcttggaca tctcctgcgc
     9241 ctggccgttg cgcaccggct cgatccacat gtgttggccc gtgccgccga cgtcgatctc
     9301 ctcgatcaac cggatcaagc gctgcacttg gctcgcggta tcggtaatga tcaggagccg
     9361 gcccgggttg tacgccgaga tatcggcttc cttgctcttg aatttggaga gaatcgggac
     9421 gacgtcgtcg ggtgtgacgt tctccagccg atagagccgt gtgatgtagc ggtcggcgtc
     9481 gggcagcggc tccccgcgtg aatataaggg tgtggccacg cccacgacgc caccgctatc
     9541 cacgatcttc agaaagcgac cgtgcggcac caccgtcatc ccgttcgcct cgaggatgga
     9601 caagaacgct tggtacgctt cctccaacgt cacgggctcg ggagagacga cggtcgcctt
     9661 cacctgacgc accttcgcgc cataaatgaa gcgacgtccc gtcaatccgc tgatgtggtt
     9721 caccagctcg gccagatccg cgtcttgcaa gttgaacttg acgagatgcc cacctggctt
     9781 cggcttgtac ggaacgtcgg ccgcagcggc gatcttatcc atattgctcg gcaagggccc
     9841 cgcggcgccg ggtggctcct tttccggagc cggtgcggcc gcaaccgccg ctgcggccgg
     9901 aggcggggtc actgccgcgg gctctgccgc tgccgccgcc ttgggtttgc gagccaagtt
     9961 gcgcaggcgc ctgcgcagaa ccgcgtcgtt ggccggagcc gctggagccg cgtcttgtgc
    10021 gagcgccgga gtcgccacgc tcaggatcag acaggacgag aggaggagcc gaaatcgtct
    10081 cacgctaaat gttctcactt gatgtggtaa tcgatgctga cggggctacc gcgccggttg
    10141 actttgatgt tcagctcgct ggccgcgcgc aggcgtgcgt acgcctccaa cgctttctcc
    10201 gggctcgcca tgttgaagcc gttgatagtc tcgagccgat cgccgttctg aaggccgagc
    10261 gtgccgagca gcgtatccgg ccgcacgccg aacagccgaa tgcccaccac cttgccgtct
    10321 ttctgttccg gcacgatccg agcgctcttc atgagctccg cctgattctc caggatcttg
    10381 tccaccacct gccggtcgac gttgaactcg ctatcgctca ccttctgaat gcgcgacgcg
    10441 atatcggccg gcacagcgcc acccgcaggc ggaggcggag gcggaggcgg agcggccgcg
    10501 accggggcgg ccgttggcgt gggttgggcc gcagacttga acagcagcac ttggcacagc
    10561 tgcccgcccg aagaacacca aactgcggga gaactctctc tgggattgaa gccgatgaac
    10621 tcgatcttct tgccgcccac gtcatccccg acccgccgca ttttgggcgc cgtctctccg
    10681 gtcgcttgca gcgcggccac cgaccacaac cgatcggtag attctgtgat gatcagagcg
    10741 ctcacgccgt cacactgggg cgccgtgaga gggtccgtgt ttgcaatcgt gtcgggtttc
    10801 ggagtcaacg cctccggttc cggcttcgga tgcagtggac ccgtcaccga gtcgaacggg
    10861 tttcgatcga tgatggcatc tgccgtgctg cgctcgcgtg acggaatcac gatcggaggc
    10921 gccggaacgg cgtgcggggc ggagccggaa ggcgccgcga gcaaagccgc tcctaacaac
    10981 tgggtggcgc cggcagcctc gaagtaggcg ctcatcgcca ccatggccag gatcaccacg
    11041 gtgaagtact tcttgatcag tgcgtcgaga gccacggggt cgccgccgtt aaagcgagag
    11101 ccgtgccaag ggtcaagccc gaaaaaaacc ttgggatttc aagcgagatc cgcgctcgct
    11161 cccgccatcc tggcaagcct cggcaggaat tttggggaag cctgacaagt tggcaaaacc
    11221 acccgtcagg cttccccctc ataccccgga actcagccgg atcaccgggc gcggcaatcc
    11281 agtcgaggag tccagccaac cctagcgagg gctaaggcct caactgggct gaccgagctc
    11341 gtgggcggcc gcctcggaga acaagcgggt gagcttcttg gcgaacgcag ccgggtcctc
    11401 gatgggactt ccttcagcga gcagggcctg atcgtaaatg agctcgagcc actctttgac
    11461 ccgaggcgag ctcgactccc tcgcattcaa cgcggtgacg ctcttgacca agggatgatt
    11521 gaggttcaac tcgaggatac ggcggctcgc aggtaggtcg cgtttttgag ccttgaacag
    11581 ccgctccaag tggggaggca gcccgccctc ggcctgaacc aaacatgccg gtgaatcggt
    11641 gagccgagac gatgccttca cgtccagcac ccggtccttc agcacttcga ggaagccctt
    11701 caataccgag gcgttcgcct cttcgtccgg cttcttctca cccgcttgtt ctccttcggg
    11761 cagcttccag tcggcgccgg taacagaaac gagcggctta tccccgtact tctcgagcgc
    11821 ggaaatggcg aaggaatcca ccggatccgt gagcaacagc acgtcatagc cccggcttcg
    11881 ggcactctcg aggtgagggc tcgagctcgc caccttctgg tttggcccga gcacgtagta
    11941 gatagcgggt tgatcactct tcatattcgc gacgtaggca tccaaactga tggacttgcc
    12001 ttgcgcgtgg ctctcgaagc ggagcagctt cgccaacttg tctttttgtt ccggctcgaa
    12061 gtagagccct tccttgagca ccaccccgaa ggtgctccag aacttgtcgt agtcgtcggg
    12121 acgttgctcg gcgatttccc cgagcagctc caaagtcttg gcgacgattt gcttcttgat
    12181 ggctttgacg gcccgcgaat cctggagggt ttcgcgggat acgttcaacg gcaggtcttc
    12241 tgagtccacc accccccgaa cgaagcggag ccaacgaggc aacagctctt ggcagttgtc
    12301 catcacgaac acgcgccgca cgtgcaggcg caccccgtgg ctggcctcgg gatcgaacaa
    12361 atcgaacggc gcgcgccccg gcacgaacag cagcgccgtg aacatttgcg tgccttcgat
    12421 ttggaagtgc ctatagccga gtggcggctc gaagtcgtgg ctctggtgct tgtagaattc
    12481 gacgtattgc tcgtgcgtga cctccttcgg cgagcgctgc cacagcgcgc taccccgatt
    12541 gatggtctcg acttccgcct ctttcttggg atcgttcgag cccacgagtt cgatcggatg
    12601 ctgcacgtaa tccgagtagc ggagcaccaa ctcacgcaga cggtacggct ccagatactc
    12661 ccgctgctcg gctttcaggt gcagcacgat gctggtcccc acgctgtcac gctgggcctc
    12721 ggaaatgctg aagccttctt ctcccgtcga ctcccacgaa taggcctggg ccgatcccgc
    12781 agcacggctc gtcaccgtga cccgatccgc caccaggtaa gcgctgtaaa aaccgacgcc
    12841 gaactgacca atcagcgtgg gcaggtcctg tgcgggcgcg ccctcgcctt tcatccggtt
    12901 gatgaactcg cgcgagccag accaagcgat ggtgcccaga ttcttggcca gctcggcctc
    12961 gctcatgccg atcccgttgt cccaaatcgt caaagtaccc gcgtcgaggt ccgcggagag
    13021 gcggatcttc agtacctccc cttcttcgag gaggctggga tccttcaggg ccgcgaaacg
    13081 cctcttatcg agggcgtccg acgcgttgga gaccagctcc cgcagaaaga tctccttgtt
    13141 gctgtagagg gaatggatca ccaggttcaa aacctggctg acctcggcct gaaaggggtg
    13201 gcgcaccgtt tccatggcga attttggcgc gaagttggcc acgtaatccg gtgtgtcaac
    13261 cccctcccga gctcactgga gctacccggt gctcgaccta ccgtccaggc aagacccacg
    13321 ccggacaacc tcgcccggca gctcgataag cagagcggtc tggctcgata agcagagcgg
    13381 tctagctcta taagcagagc ggtctagccc gacaggcgga cgagcaggct ccgcaggtta
    13441 tccaaactgg tcgcgtaggt gcgtccgtca cggggtgggt tgcaggcgtt ggcatcgtct
    13501 tgatacttcg tcacagaccc tgctcgctct agatcgagta acagtggtcc tttatccagc
    13561 accgtgcact cggtgttgga actcggagcg atctgctcga tggcctgccg cagttcggta
    13621 agttcgagct cggtgagctg acgcgcatcg ctctcgaaac gagttgggaa gccatcgcac
    13681 gtggaggtgg agagcgatga ttcctcgaag ttcacggcgt gaatcgtgac gaacttcgtc
    13741 aaatcgcact catcttccgg gctggggggc ggcgggaagc cgctcacggt gacgagcgta
    13801 agtgaacgca gcgagccgtt acccgacgtc ccgccaacgg gttccgctcc gcccgcgcct
    13861 gcttctccaa ccgctccacc aacgggcgtt acgccaccga tattccctgc gccagcgacc
    13921 ggggaagctc ctcccacggg cgacactcct cccacgggcg acgctcctcc cacgggcgac
    13981 gctcctccgc cgcttggcga gccggcgacg gatgaagcgc cccccgcaag ctgcccgccc
    14041 gaagcccgac cggccaagcc ctcgaagccg tcgcttcctc ctctgctcgg cggcacgtag
    14101 gtgccgaggt cgagttctga tcccccacag ccagcaagca gcgccaatag cgccaagcaa
    14161 gagacagttc gcatgaaacc tccgaagttc gcccgtactt cagggcgaga atagtgaacc
    14221 atgaccacgt gaacgcgacc acctgaacca cgaccacgtt cactcaataa cgatccggca
    14281 cgccctctgc gatcacggat tcgaccggcg aagccaggcc cgaagccgcg ccgagtgcgc
    14341 cccttccggc aattctcgca ggcctttttc agccaagtgg cgagcctgct ccttggcccc
    14401 cacactctcg agcgcttcca cgcgaatcac gatgctctcc ggccccaggg tgccgcgtgg
    14461 gaactcgcga tcgtaaagat cgaggatgtg tagggcccgc gcgccgcgtt gctgggccaa
    14521 cgcggcacgc gcccgatcca acaccgcaac ttcacggatc aactcatctg aagtcgaatc
    14581 tgctgccctg cgggtacgca gctcgcccgc actcgcgtct ccaggaggtt ccgggaaggc
    14641 tgcggagccc tgcaaaggag cgccatccgg ggtggccgtc gttcgatcgc tcacggagtg
    14701 cggctctccg cgcgaggtgc gatcgaggag taaggtgcga tcgaggagtg agggcacctt
    14761 ctcagcgggc tcacttcgat cagtgatcgt tcgttgcggc gagacctgat cgctctgcgg
    14821 agccacggtt cgcgaggcag cggcgcgcgc ctccactggc tttggcggct caccgatgcc
    14881 aatgggtgtt gattcgcccc cagtgcgctc ggtgaatagc tgaccttcga cgaccgcaac
    14941 cctgggcgtc gctcgcgtac tggatgcaag cggtgacgcc cccgcagccc gcgagggccg
    15001 aactcccgat gcggcacggt ccaaaccgtg accccaaccc agcgccgtcc gccactccgg
    15061 acgcgccgcg cccacgacta gccctcccac caagacccct gcgcccaaga acttcaaagc
    15121 gctgaaccag gctgctttcg agagcgctgc ggtcgcagac ggcgtcatca aactcgccgc
    15181 gctgaacgcc gccaagctgc gaaccagtgc gcgctcggag ccgacatcgc ccttcgcaga
    15241 attcagcaaa acccgatcga actcgtccag gtccgtgtcc ggcaaacgct tcgggtccgt
    15301 catcgctgat tctccctagc tccgcccgcg aaaaactcga actttgcccg ccagcggcgc
    15361 gctcgcgcgt ggaaatcttg gcgcgcccgc ctcaaacgcg aagccaccgt accaaccgga
    15421 atgcccacta gctcggcaat gtcgcccgcg ggcaaatgtt cgagctcgaa caagacgaac
    15481 acagtccgga gttcgtcggg catcgcaccc aacacctcgt ccaagcagtg acgcaattcc
    15541 cgatcgatcg ccgcttgttc aggagcgggt gaggccgcca ccaagtgctc accgtggagt
    15601 tcgtccaaat ccacgtactc gtctcgacgt tgggcgcgcc gcacctcggc cgcgacccac
    15661 actgccgtct tgaagaggaa tgcccgttca cgcccgacct cgatctgttc gcacttgcgg
    15721 gccgccacct cgaacacgcg ctgcgcggcg tcatccaccg cgtgttcggg acgtacccca
    15781 aaacgccgta agcaacgcca aatgaactga aagttttcgc gagcagccgc ctcgagccgc
    15841 gcgtgcgcgc ccgcgcgtac cggctcggac gagggcggga cgtgacggat gagcttcgag
    15901 aaggggtttc tagagagcaa taccttcgag accaacaacg cgtcgagccc gcccccgcga
    15961 tcactaaaaa agcagcgcgc ctccccaatc gaggctaacc gcgggccggt tgggtacgac
    16021 gtaaatatcg tgcgtgaccg gcgaatcgcg ctcgctgaac cgaaactccg ggcgtttcaa
    16081 aggccaatgc acaccgatgc ccgcgctgag acgcagtacc cgccgccact cccacgcaac
    16141 gagcgcgtga attccgggtg caacccacaa agacgccctc tgaaccgcgt ccggagaacc
    16201 ggtcgcacgc ccctgccccg ccagctgccc cagatccaag aacgcgcaac cacccacgag
    16261 gaagaatcgc gctcgaggcc aagaagccgc cgagcagaat tcagcccgca aagtcatcca
    16321 ggtcagctcg gctgcgccat agggctgcgc caaagtgccg ctggagacgc gcgcgccgga
    16381 aagcgctaag ccgagtacgg gcccgtgctc
//
LOCUS       DASTKU010000037         9114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_6983,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000037 DASTKU010000000
VERSION     DASTKU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9114
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6983"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2354)
                     /locus_tag="VFQ61_01240"
     CDS             complement(<1..2354)
                     /locus_tag="VFQ61_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9953092.1"
                     /translation="MKRSSTWFSQRPWPSAADVVWRPAMARVVTRVIAQVGLAAFVIC
                     MGATAGAQGRPRATSSVSGDQALERAVSELDRAQGAEVYAALRGVWETWNRADPARVE
                     EALQLAARGKELSPAGRAYAGTLAAYARLRRGDTAQARKSLEQLGYVSRFLVIGPFDN
                     EGKGGLTQNQGPELEFAQPLLTSKAYTGKERPVRWRAVPDAFPYAFVDLGALLRPETK
                     VCGLLATFVSSTTTSKAPRKISAWFGSGGAYRVFWNGVEVYTEESYSRHDFDRAAVPL
                     TLESGPNLLVVKVCGDEQAPTFSLRLAEPDGSPAQGLSNSNDLALAEAAAELAKQALA
                     AHDKTEKSGKKAAVKPAPRAQGPLPAFERLTDRANASAADLEAHARYLVATDGDDPSQ
                     HLARDLSRRAADKEPTIRRLLLASRLAEDRNQASEWLEKAEKLAAASGKPDLDLLLAR
                     AWHRRHGPNFREALPLFQKALAIDPTNVEALRGTLELYNLAGLSRTALAEIERASEQN
                     PNSVGLLALLASQLRTLGRTTDAMEVEARYHGLRADDTGYLNQMLELSLERNDRTSAL
                     HFADRLLQAEPHDAWVLGTAARAYRRLGEPDRALSTHRAALALAPEDVGSLRAMSDLQ
                     GELGNRGEQLSLLRELVRVRPQDKQVREYIENMEPEKPRADEAHAYGPDKFLYLRHAP
                     AAGQSRRTLRDLTVSTVYQNGLSSQFRQVVFQPLTDAAAAQDRQYAFAYEASQQVVQL
                     RGAKVYRKNGKVDEAIEWGEGPADDPSIAMYTSARTFYVQFPRL"
     gene            3061..3543
                     /locus_tag="VFQ61_01245"
     CDS             3061..3543
                     /locus_tag="VFQ61_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET9953093.1"
                     /translation="MVNPQMVMYEEEFNQIQAVVDRLVREANAKVVFIVDKNGQLIAA
                     SGEVENIDTTSLASLTAGNIAATGGIAKLLRENEFATQFHEGERANIHIQLVGNRVIL
                     VVIFDSKSSLGLVRLRVRKASDELNRIFEALLSKVQEPGADSPFGEITDEDIDNLFND
                     "
     gene            3553..4140
                     /locus_tag="VFQ61_01250"
     CDS             3553..4140
                     /locus_tag="VFQ61_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HET9953094.1"
                     /translation="MSFINYMAREINCKIVYYGPGLCGKTTNLQYIYERTNPDAKGKM
                     ISLATETERTLFFDFLPLSLGEIRGFKTRFHLYTVPGQVFYDASRKLILKGVDGVIFV
                     ADSQIERLEANQESVENLRTNLGEQGYNLDKLPYVVQYNKRDLPNVVPVEELRALINT
                     TNVPDFEANARAGTGVFDTLKAVSKLVLNELRRGG"
     gene            4170..4823
                     /locus_tag="VFQ61_01255"
     CDS             4170..4823
                     /locus_tag="VFQ61_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HET9953095.1"
                     /translation="MPRATRAPKTPPPLTPTRLADELLRYLNQRDASRSRAVTHLQRW
                     ITRHYGAERVAAERPHIDALLQRFMASGVLDDGRLARHLVESFRRRGASQRAIAQKLR
                     ARGLDSATIEGAFESEASERSERAPRPRELANTSVGAAVAPEAPAYSNRRAARSSGSA
                     ELEAAREFVRKRRIGPHRPEPERAPNRRKDLAKLARAGFDFDTACSALGGHFSEDDF"
     gene            4883..5938
                     /locus_tag="VFQ61_01260"
     CDS             4883..5938
                     /locus_tag="VFQ61_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET9953096.1"
                     /translation="MPTRVSPMLRTGKVTSELRRLAYALSVFASIVFAGGSMLFVLSG
                     AEYGFWECVYFALVTSASVGFAELPAMEVHPGLRFVTSLMIVTGVGTIAFFQSNLTAL
                     LVEGFLGETFRRRRMDQKIAKLSGHFVVAGCGRTGRYIVEELHATRHQFVVVDRDIET
                     LRRLNEELEGELLYIVGDATLDHTLIAARIGTAAGVMAALTDDRDNLFIVLSSRALNP
                     NARIVSKVVEIENDSKILRAGADRTVSPHRMGGMRMVNELVRPRVVSFLDQMLRDDKN
                     LRFEEVELSATSRYVGKTLREIPIRKETQLLVVALHVDSKYVYNPAPDHALTAGTRLI
                     VMGDLPNVEKLRRLLET"
     gene            complement(6299..6622)
                     /locus_tag="VFQ61_01265"
     CDS             complement(6299..6622)
                     /locus_tag="VFQ61_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9953097.1"
                     /translation="MQSGRKNERVTINKEFDSFDQFMSEYVTNLSRSGAFIRTSTPLP
                     IGTEIDLRFTVVMDGIETIEGIGEVVRLETSPPGMGVIFKSLNPGSQALIDKLLAQKA
                     EEPGP"
     gene            complement(6650..8914)
                     /locus_tag="VFQ61_01270"
     CDS             complement(6650..8914)
                     /locus_tag="VFQ61_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HET9953098.1"
                     /translation="MPRNTLVINCDIRETRVALIEGGVIGELQIERASSRGTVGNIVL
                     GRVTRVLPGMQAAFIDVGMERAAFLHVEDLIRPDDFEAYLAGARRHDEGERWNKGEGG
                     RRRATSSGPGKEDDDDDDDEDLTPESVASESRIQTSLLDRETEAVDLDDDAELGDEEE
                     EEEEEEEGEEQDVDPGEDDDPEAEAATLVTETEALVTEVLVPPDVDVEGDDEPLLDNG
                     DEDEVVPGAAKEAGSEPDGASSRRKRRRRRRRGREAEGQTSSPVAAAAPVTEARREEP
                     RREEARKDEPRKEESRKEESRKEESRREVREKRAARAGRDARGSREARESRESRESRE
                     TKGDADARRPKGILRVSRSVPITEVVKEGDEVIVQISKEPIGTKGARVTSHVSLPGRY
                     VVYLPTVDHVGVSKRIGSARERTRLRQVVDTLKPPTGGIIVRTLAEGLTKKDLKSDIG
                     YLVRLWEDVLKKRATGKAPCVLYTELDLVLKTARDLFTDEIESIVIDDKAEYARLVRF
                     VEMFLPARVKDIHYYAGDEPIFDAYGIEDEISRALSRKVPLPSGGYLIIDQAEALTAI
                     DVNTGRFVGKGSKDLEETALKTNLEAVEEIAYQLRFRNIGGLVILDLIDMDVSSNREK
                     VWRALEQRLAKDKAKTTINRISELGLIEMTRKRTRESLTRTMHETCFYCDGTGQIQSR
                     GTIAYEILRQIRRERHNLQGYSVVVNAHPAVVDLMTGEEREAVAEAERRYMRRIEFNP
                     RREYHIEQFDLQGK"
BASE COUNT         1594 a         2977 c         2834 g         1709 t
ORIGIN      
        1 tcgaggcgcg ggaactgaac gtagaaggtg cgcgccgacg tatacattgc aatcgacggg
       61 tcatccgccg ggccttcgcc ccactcgatg gcctcgtcca ctttgccgtt cttgcggtag
      121 accttggcgc cgcgcagctg caccacctgt tgcgatgctt cgtaagcgaa ggcgtattgt
      181 cgatcttgag ccgccgccgc gtccgtcagg ggctgaaaca ccacctgacg gaactggctc
      241 gaaagcccat tctggtagac ggtcgacacc gtcaaatcgc ggagcgtacg gcggctctga
      301 cccgcggcgg gcgcgtggcg caaatacagg aacttgtcgg ggccgtacgc gtgtgcttcg
      361 tcggcgcgcg gcttctcggg ctccatgttc tcgatgtact cgcggacttg tttgtcctgc
      421 ggccgcaccc gcacgagctc tcgtaataga gagagttgct cgccgcgatt gccgagctcg
      481 ccctgcaaat cggacatcgc ccgcaggctg cccacgtctt cgggggcgag cgccaaagcc
      541 gcgcgatgcg tgctcagcgc ccgatcgggc tcgccgagcc gccgataggc ccgtgcggcc
      601 gtgcccaata cccaggcgtc gtggggctct gcctgcaaca agcgatcggc gaagtgcaac
      661 gccgaggtgc gatcgttgcg ctcgagtgac agctcgagca tctggttcag gtagccggta
      721 tcgtcagcgc ggagcccgtg atagcgcgct tcgacctcca tcgcgtccgt ggttcggccc
      781 aatgttctga gctgcgaggc gagcaaagcc agcagcccca ccgagttcgg gttctgctcc
      841 gaggcgcgct cgatctccgc caacgccgtg cgcgacaacc ctgcgaggtt atacagctcg
      901 agcgtgcccc gcagcgcttc cacgttggtc ggatcgatcg cgagcgcctt ctggaacagc
      961 ggcagcgcct ctcgaaagtt cggaccgtgg cgcctatgcc acgcacgcgc gagcagcaaa
     1021 tctagatccg gtttgccgct cgcggccgcc agcttctcgg ctttctcgag ccactccgaa
     1081 gcctgattgc ggtcctccgc caagcgtgac gccagcagca agcgccgaat ggtgggttcc
     1141 ttgtcagcgg cgcgccgcga gagatcgcga gccaagtgct gagatggatc gtcaccatcc
     1201 gtcgcaacca ggtaacgcgc atgcgcctcg agatccgcgg cggacgcgtt ggcccgatcc
     1261 gtcaggcgtt cgaacgccgg cagcgggcct tgggcgcgcg gagccggttt tactgctgct
     1321 tttttcccgg atttctcggt tttgtcgtgg gccgcgagcg cctgcttcgc gagttcggcg
     1381 gccgcctcgg ccagcgctaa atcattcgag ttcgagagcc cctgtgcagg tgagccgtcc
     1441 ggctccgcca agcgcaggga gaaagtgggc gcttgctcgt cgccgcaaac cttcacgacc
     1501 agcaagttgg gaccgctctc gagcgtgagc gggaccgcgg cgcggtcgaa gtcgtgacgc
     1561 gaataggatt cctccgtgta aacctcgacg ccgttccaga acacgcggta agccccgccg
     1621 ctcccgaacc aggcgctgat cttgcgagga gccttcgagg tcgtcgtgga gctcacgaac
     1681 gtggcgagca agccgcacac tttggtttcc ggccgcagca acgctccgag atccacgaaa
     1741 gcgtacggaa acgcgtcggg caccgcgcgc caacgcaccg ggcgctcctt gcctgtgtat
     1801 gccttggacg tgagcagcgg ctgagcaaac tcgagctcgg gcccttggtt ctgcgtcagc
     1861 ccacctttgc cctcgttgtc gaatgggcca atcaccaaaa atcggctcac gtagccgagt
     1921 tgctcgaggc tcttacgtgc ctgcgcggtg tcgccacgcc gcaaacgtgc gtatgccgcc
     1981 agtgtgcccg cgtaggcgcg gcccgccggt gagagctctt tgccccgtgc cgcaagctgt
     2041 aacgcttcct cgacccgggc gggatcggcg cggttccatg tttcccaaac gccgcgcagc
     2101 gcggcgtaca cttcggcacc ttgcgcccga tcgagctcac tgaccgcgcg ttccagagcc
     2161 tgatccccgc tgaccgaaga cgtcgcgcgc ggccgccctt gagcccccgc ggtagccccc
     2221 atgcagatga cgaatgccgc gaggccaacc tgcgcgatca cgcgcgtgac gacccgagcc
     2281 atcgcggggc gccacactac gtctgcggca gacggccagg gccgttgtga aaaccaagtc
     2341 gaggaacgct tcatgcgggc cttgccatcc accgctctcg ggtaccttca ggctgcaccc
     2401 gacgcaagtt aaccaggttc tgctcgccca aagggcacgg ccatgtgcgc cccggcccca
     2461 caccctcgaa cgacccccgc gacagcagcg tggacgctcg cgcggccgag cgctcgggtg
     2521 gtgaaccaaa gccctccgcg gccgcgtctt tctcttaaga atcgaaggga attggccgca
     2581 gagttggctg tggcacctga atggtcagtg agctcggcat cgagccgact cgctcgacca
     2641 gtacttggct cggccaagcg cattcggcgc cggcaccccc aaacttatcg aactagcgcg
     2701 tcaacacgaa tcccgaacag cccgcgggcc gagctctttt gctcgtcgcg ctcggcggtt
     2761 cgagagacgt ggctcgaacg cgcaaggaaa agcatgctgg caaagtcgcg gtgcaaggcc
     2821 gcgctgccga aagcgcgctg cggaaagccc gctgcggaaa gcagagcgat ggggcttgct
     2881 gggtcgggga cggtcatata gaagtcgaac gttacggaaa ttctttgaat gaccaaccct
     2941 tcgccgggtg cgtcgccgaa acgctataac cctgaatcgt agtaacccga agcatagcta
     3001 ggcgactcgg tttgaagacg gcacgggaga gtccgttcga aggaccctcg gaggcttgag
     3061 atggttaacc cgcagatggt gatgtacgaa gaggagttca accagatcca agcggtcgta
     3121 gaccgtttgg tgagggaagc gaacgccaag gtcgtattca tcgtcgacaa gaatggtcag
     3181 ttgatcgcgg ccagtggtga agtcgagaac atcgacacga cgtccttggc gtccctcact
     3241 gcgggcaaca ttgctgcgac cggcggcatc gccaagttgc tgcgtgagaa cgaattcgcc
     3301 acgcagtttc acgaaggcga acgcgccaac attcacatcc agctcgtcgg gaaccgggtc
     3361 atcctggtcg tgatcttcga ctccaagtcg agcctcggac tggttcggct cagagtgcgc
     3421 aaagccagcg acgagctaaa tcgaatcttc gaagcgctcc tcagtaaagt tcaggagcca
     3481 ggtgccgatt caccttttgg ggaaatcacc gacgaggaca tcgacaactt gttcaacgac
     3541 tgaggccggg cgatgagctt catcaactac atggcccggg agatcaactg caagatcgtg
     3601 tattacggcc cgggcctctg cggcaagacc accaatctcc agtacattta cgagcgcacc
     3661 aaccctgacg ccaagggcaa gatgatcagc ttggcgacag agacggagcg cactctgttc
     3721 ttcgacttcc tgccgctctc gttgggcgag attcgcggat tcaagacgcg cttccacctg
     3781 tacacggtac ccggccaggt cttctacgac gcgagccgta agctgatcct gaagggtgtg
     3841 gacggcgtaa tcttcgtcgc ggactcgcag atcgagcgtc tggaagccaa tcaagagagt
     3901 gtcgagaacc tgcgcaccaa cttgggcgag cagggctaca acctcgataa gctcccttac
     3961 gtcgtgcagt acaacaagcg tgacctgccg aacgtggtgc cggtagagga gctgcgggca
     4021 ctcatcaaca ccacgaacgt gcccgatttc gaggccaacg cgcgcgccgg caccggtgtg
     4081 ttcgacaccc tgaaggcggt cagcaagctg gttttgaacg agctgcgccg cggcggctga
     4141 gcggcgcgca ccgcaaactc gaactgggca tgccgcgcgc gacgcgagct ccgaagacgc
     4201 cgccgccgct gacgccgact cggcttgccg acgaactgtt gcgttatctg aatcagcgtg
     4261 acgcgagtcg cagccgcgcc gttactcacc tgcagcgctg gattacgcgg cactacggcg
     4321 cggagcgcgt ggcggccgag cgtccccaca tcgacgcgct gctgcagcgc ttcatggcct
     4381 cgggcgtgct cgacgatggg cgcctagccc ggcacctggt cgagagcttc cgcagacgcg
     4441 gagcatccca gcgcgccatc gcgcaaaagc tccgggcgcg cggcctcgac agcgccacca
     4501 tcgagggggc gttcgaatcg gaagccagcg agcgcagcga gcgcgctcct cgtccgcgag
     4561 agctcgcgaa cacgagtgta ggagccgcag tcgcaccaga agcgccggcg tactccaacc
     4621 gtagggcggc acggagcagc gggtctgcgg aactcgaggc ggcgcgagag ttcgtgcgca
     4681 aacgccgaat cgggccacat cgtccggaac ccgaacgagc tccgaatcgg cggaaagatt
     4741 tggccaaact cgcgcgcgcc ggcttcgact tcgataccgc ttgcagcgcg ctcggtggtc
     4801 acttctccga agatgacttt tgacgttaga gccctccgcc ggtagcggag ctggcagcgc
     4861 ccgagaacgc atacggtagg ctatgcctac gcgcgtttcc ccgatgctgc gaaccggcaa
     4921 agtcacgtcc gagctccgac gcttggctta cgcgctgagc gtctttgcca gcatcgtgtt
     4981 cgccggcggg agcatgctgt tcgtgctgag cggggcggag tacggctttt gggagtgcgt
     5041 gtacttcgcc ctcgtcacct ccgccagcgt ggggttcgcc gaactgccgg ccatggaagt
     5101 gcacccgggg ttgcgcttcg tcaccagtct gatgatcgtg actggagtcg gcacgatcgc
     5161 gttctttcag tccaacctga ccgcgctgct cgtagaaggc ttcttgggtg agacgttccg
     5221 gaggcgtcgc atggatcaga agattgccaa gttgtcgggc cacttcgtgg tggctggctg
     5281 cggccgcacc gggcgctaca tcgtcgaaga gctccacgcc actcgccatc agttcgtggt
     5341 cgtcgatcgt gacatcgaga cgctgcgacg tctgaatgaa gagctcgagg gagagctgct
     5401 gtacatcgtg ggggacgcga cgctcgacca caccttgatc gccgcccgca tcggcaccgc
     5461 ggcgggcgtg atggccgcac tcaccgacga ccgcgataac ctcttcatcg tgctttcgtc
     5521 gcgcgcactg aacccaaacg cgcggatcgt ctcgaaggtg gtcgaaatcg agaatgactc
     5581 gaagatactg cgcgcgggcg cggatcgcac ggtcagcccg catcgcatgg gcgggatgcg
     5641 catggtgaac gagctcgtgc ggccccgggt cgtgagcttt ctcgatcaga tgctgcgcga
     5701 cgacaagaat ctacgcttcg aagaggtgga gctcagcgcg acctctcggt acgtgggcaa
     5761 aactctgcgc gagatcccga tccgtaaaga gacgcagctg ttggtggtcg ccctgcacgt
     5821 cgacagcaag tacgtctaca accccgcgcc cgatcacgcg ctcaccgccg gaactcgctt
     5881 aatcgtgatg ggcgacctgc cgaacgtcga aaagctgcgg cggctgctcg aaacctaagc
     5941 ctgaagaaac gccaggcccg ccgtggagcg ggcctacgag gcgttcgagc tttcaggacg
     6001 gacgtggatg ggtgctcacc cggcccaagc tccttcgact gagctgagtg gcagcgagct
     6061 gagtcgcagc gagctgagtc gcagcgagct ggaatcctcg cagcggcaat cacgaggccg
     6121 ctgcgagacc gctcacttgc cagccatacg gttcttgcga ttgcggttct tcgcgcgcag
     6181 cttggcgcgg tgacgcacgg agcgtttgcg gtcggtcttg cggggcgact tggtgcgggc
     6241 gagatacatg gcggcgcgtt cttaattcga accgagtggg cgcgcaagcc cctcgggcct
     6301 aggggccggg ttcttcggct ttttgtgcga gaagtttgtc gatcaaggcc tgcgagcccg
     6361 ggttcaggct cttgaaaatg acccccatgc ccggcgggct ggtctcgaga cgcaccacct
     6421 caccgatccc ttcgatggtc tcgatgccat ccatgacgac ggtgaagcgg agatcgattt
     6481 cggtcccgat cggtagcggc gtgctggtcc gaatgaacgc gcccgatcgt gaaaggttcg
     6541 tcacgtactc actcatgaac tgatcgaacg agtcgaactc cttgttgatg gtgacccgtt
     6601 cgttctttcg accgctctgc atgtgagcct ccctgagctg ccttggccgc tacttgccct
     6661 gaagatcgaa ctgctcgatg tggtactccc gacgcgggtt gaactcgatg cgtcgcatgt
     6721 agcggcgctc tgcctctgcc accgcctcgc gctcctcacc ggtcatcaag tccactaccg
     6781 cagggtgggc gttcaccacc accgagtaac cctgcaggtt gtgccgttcg cgccggattt
     6841 ggcgcaagat ttcgtaggcg atcgtgccgc gcgactgaat ctgcccggta ccgtcgcagt
     6901 agaagcacgt ctcgtgcatg gtccgggtca agctctcgcg cgtacgcttc cgcgtcatct
     6961 cgatcagccc aagctcgctg atgcgattga tcgtggtctt ggccttgtcc ttggccaaac
     7021 gctgctcgag tgcccgccac accttctcac gattgctcga cacatccatg tcgatcaaat
     7081 cgaggatcac gagcccgccg atgttgcgga agcgcagctg ataggcgatc tcctcgaccg
     7141 cctcgaggtt ggtcttgagc gccgtctcct cgagatcttt gctgcccttg cccacgaaac
     7201 ggccggtgtt cacgtcgatt gccgtgagcg cctccgcctg atcgatgatc aagtatccgc
     7261 cgctcggcag cggcactttg cggctcagag cgcgagaaat ctcgtcttcg atgccgtaag
     7321 cgtcgaagat gggctcgtcg cccgcgtagt agtggatgtc cttcacgcgc gccggcaaga
     7381 acatctcgac gaaccgcacc agccgcgcgt actcggcctt gtcgtcgatc acgatgctct
     7441 cgatctcgtc ggtgaacaga tcgcgcgcag tcttcaggac caagtccagc tcggtgtaca
     7501 gcacgcacgg agccttgccc gtggcgcgct tcttgagcac gtcttcccaa agccgaacca
     7561 ggtagccgat gtcggacttg agatctttct tggtcaggcc ctcggccagc gtgcgcacga
     7621 tgatgccacc cgtcgggggc ttcaacgtat ccaccacctg acgcaagcgc gtgcgctcgc
     7681 gcgccgagcc gatgcgcttg gagactccaa cgtgatcgac cgtcggcaag tacaccacgt
     7741 agcgccccgg caacgagacg tggctcgtca cacgcgcgcc cttcgtgccg attggttcct
     7801 tggaaatctg gacaatcacc tcgtcgcctt ctttgacgac ctcggtgatg gggaccgatc
     7861 gcgagacacg caggataccc ttcggccgcc gcgcgtctgc gtcccctttg gtctctctgg
     7921 attcgcgtga ctcccgcgat tcgcgggctt cacgggaccc acgcgcgtca cgcccggccc
     7981 gcgccgcgcg tttctcgcgc acttcccggc gcgactcctc cttacgcgac tcctccttac
     8041 gcgattcttc cttgcggggc tcgtctttgc gggcctcctc acgccggggt tcctcgcgtc
     8101 gtgcctccgt gaccggcgcg gccgcggcta ccggcgatga cgtctgacct tccgcttcac
     8161 gcccgcggcg gcgccgacgg cgccgtttcc ggcgcgacga agcaccgtcg ggttccgaac
     8221 cggcctcttt ggcggcgccg gggacgacct cgtcctcgtc accgttgtcg agcagcggtt
     8281 catcatcacc ctccacgtcg acgtccggcg gcaccagtac ctccgtcacc aaggcttcgg
     8341 tctcggtgac caaagttgct gcctcggctt cggggtcgtc gtcctcaccg ggatcgacgt
     8401 cctgctcctc gccctcttct tcctcctctt cctcttcctc ttcgtcgccg agttcggcgt
     8461 cgtcgtcgag gtctacggct tcggtttcgc ggtcgaggag cgaggtttgg attcggctct
     8521 cactcgccac gctctcgggc gtgagatcct cgtcgtcatc gtcgtcgtcg tcttctttac
     8581 cgggaccaga tgaggtggct cttcgccgcc ccccttctcc tttgttccat cgttcgcctt
     8641 catcatggcg gcgtgcgccg gccagatagg cttcgaagtc gtctgggcgg atcagatcct
     8701 cgacgtgcag gaacgccgcg cgctccatcc ctacatcgat gaatgcggct tgcatccccg
     8761 gcagcacgcg tgtcacgcgg ccgagcacga tgttgcccac ggtgccccgt gagctggcac
     8821 gctcaatctg aagttcgcca atgacgccac cctcgattag ggcgacgcgg gtttcgcgga
     8881 tgtcgcagtt aatgaccagt gtatttcgag gcatgactcg cccgaccggg ggcgggcccc
     8941 agcgcagttc atgggccggg ggaagagacg tcccgcttac cacgaggcgg ggaccttggt
     9001 gccgccggcc cgcgtggttc ggccacgtga ccacgtcaaa ggcgttctgt caatgctctc
     9061 gaaataactg gtgatttacc ggggcactag ggccgatttc cagactgcct caga
//
LOCUS       DASTKU010000038         6688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_453103, whole genome shotgun sequence.
ACCESSION   DASTKU010000038 DASTKU010000000
VERSION     DASTKU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453103"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFQ61_01275"
     CDS             complement(<1..218)
                     /locus_tag="VFQ61_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953099.1"
                     /translation="MTSPGTEYVSLSGPWGPPGPGGPGHPNYPGGGYPGGGHPGGGHP
                     GGGAGWGGAWGPGAPGGGQGSGSGHGGG"
     gene            340..633
                     /gene="gatC"
                     /locus_tag="VFQ61_01280"
     CDS             340..633
                     /gene="gatC"
                     /locus_tag="VFQ61_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET9953100.1"
                     /translation="MAITRETVLHVARLARLDLDESEVERMKRDLNSILEYVDELSSV
                     DTSAIAETAHISLESAVLRPDLSKPGVATELALSEAPRGSGSSFAVPAFVEET"
     gene            630..2123
                     /gene="gatA"
                     /locus_tag="VFQ61_01285"
     CDS             630..2123
                     /gene="gatA"
                     /locus_tag="VFQ61_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET9953101.1"
                     /translation="MSALDRSIPEIVDGVTRGELRASTLLEESLARIEKHSNLNAFLS
                     LSADSAREQVARIEADVAQGRPLGKLAGVPFAVKDALCTSDAPTTAGSKILTKAAKDG
                     SLAGPSRGWRPPYDATVVARLRAAGAVIVGKTNMDEFAMGSSGENSAFGAPRNPHDPT
                     RTPGGSSGGSAVAVAAGMTPASLGSDTGGSIRQPAALSGCVGVKPTYGRVSRFGLIAF
                     ASSLDQVGPFARDVVSAARVMSVISGHDVRDSTSSTRPVADYEAACQRDVKGLRIGVP
                     EEYFGEGLDPEIASSIRNAIAKLERDGVSVKPIALPHTRYGIATYYIVATAEASSNLA
                     RFDGVRFGLRREPAGADLAAMYGATRDQGFGAEVKRRILLGTYVLSAGYYDAYYRKAQ
                     RVRTLIRQDFDRAFEQVDVIAAPTSPTPAFKLGEKIEDPLAMYLADIYTLPASLAGIA
                     GLTLPCGMTAARSDRPALPIGLQLLSPSFEEERLFSVAAAVERSNRA"
     gene            2120..2737
                     /locus_tag="VFQ61_01290"
     CDS             2120..2737
                     /locus_tag="VFQ61_01290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9953102.1"
                     /translation="MTHELRAELARAEDIPEIEQLFRAADVPCYCQYWHFTGDKNAWF
                     ARCAFEADQNLRELEQRLRTPESSPQAVLARGPDGVVGWMKLCRAADVPKLFEQRVYR
                     SLRCFTGDRDRTWVVGCFLIHPDWRRCGVADKLLERGIEIARQMRAHGIEALPRRGEG
                     LSEPEMCAGPYDLFVKHGFRIVDESYLPYPVLRIDLAVSDATPEP"
     gene            complement(2683..4686)
                     /locus_tag="VFQ61_01295"
     CDS             complement(2683..4686)
                     /locus_tag="VFQ61_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9953103.1"
                     /translation="MKLGPYVLGERLGLGGMAEVYVGHRAGPHGFAKRFAIKRILPEL
                     AKDPRFLAMFVDEARICAALQHPNIVQVVDFGESEGELFMAMEFVDGVSVAKLLRNVA
                     GKRERFPISIVLLVVRDVLRGLAFAHEATNEHGQPLGLVHRDVSPGNVLISRAGEVKL
                     ADFGIVRSALVDRRTNPGELKGKLGYMSPEQVIGAEVDPRSDLFAVGILLCEMLITCP
                     LFSGKNELEVLTRIHDADLTTFERHGGHVPEPLAEIIRHALAREPRQRFQSAREFGAA
                     IEAYADQNRISLDSAELAGWLSGLGILPSRSGMHSLNAEPPKAEPNVPPEPSPASEIT
                     RDAAPRREHRRPTPQGLPVDDGRGAGRVDLLEYVCTGRVRRRTKLVGRDGIGRPASEV
                     AGTAELLSRPAFQFGEPEFTQAEWTREIRARELPARIYALLQSDATGLLVARAGAREK
                     RIFITQGTLRFVATTEREELLGHRLLRRGVLKETELRPALEARARGGGHLGEVLIATG
                     AIGPTTLLRELIEQLEERFLELFHWREGTLAFFPGLTSGEQELGVPSSISLVTRGVRA
                     HYPGEELALLLGHTAHTPILAGPRADTDLNRLGVSHAERLILERAVSAGTLEQLTSVA
                     AAERLGTLEETLRAVFVGLSTGIFEIHGSGVASDTAKSIRSTG"
     gene            4991..6040
                     /locus_tag="VFQ61_01300"
     CDS             4991..6040
                     /locus_tag="VFQ61_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9953104.1"
                     /translation="MSTETTPPPIERPSSPLSRPLQRQAVGVTGASTFLGANLVGMLE
                     EDDAVRRIVCLDSKAPSTAGAKSRAYDVDLTQSASEERLTEILSAEAVDTLVHLAFLP
                     SPTHTSAFAHELESVGTMRALNACRRANVRKFVMWSQTFLYGAHPTNPNYLSEKHPLR
                     APRGEAYFQDKLDAEREVQSFGRPGNGKVATILRTSPILGPNVDTFLTRYFSRKWVPT
                     VLGFDPLWQFLHEADAVTAFRQAVLRDAPGTFNVTGDGVLPLHTAIRLIGRSPLPMPR
                     TFTRNMASALWVAELCEAPPSFCDFLQYLCVADGALAKRALGFEPMYTSREAVIEFGN
                     AQRLRDVRLLSERPA"
     gene            6220..>6688
                     /locus_tag="VFQ61_01305"
     CDS             6220..>6688
                     /locus_tag="VFQ61_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953105.1"
                     /translation="MSRPPVRKPRPDRATPPLGTGHRSPFNPPVERPHDRGRERDEEP
                     RAYPPRAADHAIHDEAPSSEWQDQFSDYLDEEVPELGVGSRMVPPEVRYEEKPEPAPV
                     PKPLTPVDFEAQIRTLEARLDGLIRRATLAEESEAEAAPASFRSPGPQVEATPA"
BASE COUNT         1154 a         2232 c         2134 g         1168 t
ORIGIN      
        1 tagccgccgc cgtggccact accggaaccc tgaccacccc cgggagcacc aggcccccat
       61 gcgccccccc agccagctcc accgccggga tgaccaccgc cgggatgacc accgccggga
      121 taaccaccgc cgggataatt cggatgacct ggtccgccgg gcccgggtgg gccccacggg
      181 ccggagaggg agacgtactc ggtgccggga gaggtcacgg ttccgagcat agccggttca
      241 tggtgcgcga gctggtgttt cgcggccggg gtgatcccag cgtcggctgc gcgagcggtc
      301 ttctgaaatg cgcgtcacta gtgctaggct ccgcgcccca tggcgatcac ccgtgaaacc
      361 gtgctccacg tcgcgcgtct ggcgcggctg gatctcgacg aaagtgaagt cgagcgcatg
      421 aagcgcgatc tgaacagcat cctggaatac gtggacgagc tctcgtccgt ggataccagc
      481 gcgatcgcag agactgcgca tatttcattg gagtctgccg tgctccgccc ggatttgagc
      541 aagcccggcg tagctacgga actcgcattg agcgaggcgc cgcgcggctc tggctcgtcg
      601 ttcgccgtcc ctgccttcgt ggaagaaaca tgagtgcatt agaccgaagc atccccgaga
      661 tcgtggatgg tgtgacccgg ggcgagttgc gtgcctctac gctgctcgaa gagtccttgg
      721 ctcgcatcga gaagcactcg aacctgaacg ctttcctgag tctgtcggcg gattcggcgc
      781 gcgagcaggt ggcgcggatc gaagcagacg tggcgcaagg gcgtccgctc ggcaagctcg
      841 cgggcgtacc gttcgcggtc aaagacgcgc tgtgtacctc ggacgcaccg accacggccg
      901 gttcgaagat tctcaccaaa gccgccaagg atggctccct ggcaggccca tcccgaggtt
      961 ggcggcctcc ctacgacgcg accgtggtcg cgcgcctgcg cgctgcgggc gcggtcatcg
     1021 tcggcaagac gaacatggat gagttcgcga tggggtcgtc gggcgagaac agcgctttcg
     1081 gtgcgccacg caacccccac gatccgaccc gcacgcccgg cggttcgagc ggaggcagcg
     1141 cggtcgcggt ggcggccggc atgactcccg cttcgctcgg aagcgacacg ggcggctcca
     1201 tccgccagcc cgccgcgctg agcggctgcg tcggagtcaa gccaacttat gggcgcgtct
     1261 cacgcttcgg cttgatcgcc tttgcttcca gcttggatca ggttgggccg ttcgcgcggg
     1321 acgtggtgag cgccgcgcgc gtgatgagcg tcatctccgg gcacgacgtg cgagactcga
     1381 cgagctcgac acgaccggtg gcagactacg aagctgcttg ccagcgtgat gtgaagggcc
     1441 tgcggatcgg cgtgcccgag gagtacttcg gggaaggtct cgatcccgag atcgcgagtt
     1501 cgatccgcaa cgcgatcgcc aagctcgaac gcgacggcgt ttcggtgaag ccgatcgcgc
     1561 tgccgcacac gcgctacggc atcgcaactt actacatcgt cgcgaccgcg gaggcgtcca
     1621 gcaacttggc acgcttcgac ggtgtacgct ttggattgcg gagagagccc gcgggcgcgg
     1681 atctggcagc catgtacggc gctacccgcg accaaggctt cggtgccgag gtgaagcgcc
     1741 gaatcctgct cggaacgtac gtgctcagcg cgggctacta cgacgcctat taccggaagg
     1801 cgcagcgcgt gcgcacgctg atccggcaag acttcgatcg cgccttcgag caggtggacg
     1861 tgattgccgc gcccacgagc ccgacccctg ctttcaagct cggggagaag atcgaggatc
     1921 cattggccat gtacttggct gacatctata cgctgcccgc gagcctggcg ggcattgccg
     1981 gcctgacctt gccttgcggg atgaccgccg ctcgtagcga tcgtccggcg ttaccgatcg
     2041 ggctgcagct gctcagcccg tccttcgagg aagagcggct attcagcgtc gcggcggcag
     2101 tggagcgctc gaatcgcgcg tgactcacga gcttcgagcg gaactcgcgc gggccgagga
     2161 tatccccgaa atcgagcagc tatttcgggc cgcggacgtg ccttgttatt gccagtattg
     2221 gcacttcacg ggcgacaaga acgcctggtt cgcgcgctgc gcgttcgagg cggatcagaa
     2281 cctgcgcgag ttggagcagc ggcttcgcac gcccgagtcg agtccacaag ccgtgctcgc
     2341 acgagggccc gatggcgtag tcggttggat gaagctatgc cgcgccgccg atgttcccaa
     2401 gctgttcgag cagcgggtct atcgcagcct gcgctgcttc accggagacc gcgatcggac
     2461 ctgggtggtg ggctgtttcc tgatccaccc ggattggcga cgttgcggcg tggccgataa
     2521 gctgctcgag cgaggcatcg agatcgcgcg gcagatgcgt gcgcacggga tcgaagcgct
     2581 cccccgacgg ggtgagggat tatccgagcc cgagatgtgc gcggggccct acgatttgtt
     2641 cgtaaagcac ggatttcgca tcgtggacga gagctacctg ccttacccgg tgctgcggat
     2701 tgatttggcg gtgtccgacg ccactccgga gccgtgaatc tcgaatatac ccgtcgaaag
     2761 gcccacgaag accgcgcgca gcgtttcttc caaggtaccg agccgctccg cggccgcgac
     2821 gctcgtcaat tgttccagcg ttcctgccga gactgctcgc tccagaatga ggcgctcggc
     2881 atgcgagacg ccgagtcgat tcaggtcggt gtctgcgcgc ggaccggcga ggatcggcgt
     2941 gtgcgcggtg tgtccgagca gaagtgcgag ctcttcgccg gggtaatgag ctcgtacgcc
     3001 gcgcgtcacg agactgatcg aggacggcac cccgagctcc tgctctccgc tcgtcagtcc
     3061 tggaaagaac gccaaggtgc cttcgcgcca gtggaacagt tcgaggaaac gttcttccag
     3121 ctgttcgatc agctcgcgca gcagcgtggt cggaccaatc gcgccggtcg cgatcaagac
     3181 ttcgcccaag tggcccccgc cgcgggcgcg cgcctcgagc gcgggacgaa gctcggtctc
     3241 tttgagcaca ccgcgccgga gcaagcgatg cccgagcagc tcttctcgtt cggtcgtagc
     3301 gacgaagcgg agcgtgcctt gcgtgatgaa gatgcgcttc tcacgagcac cggcgcgggc
     3361 cacgagcaag ccggtggcat ccgactgcag cagagcgtaa attctggcgg gcaactcgcg
     3421 agcccgaatt tcccgggtcc actcggcctg cgtaaactcc ggctccccga actggaaagc
     3481 gggtcgcgac agcagctccg cggtaccagc tacttcggaa gcgggccgcc caataccgtc
     3541 gcggccaacg agcttcgtgc ggcggcggac gcggcccgtg cagacgtact cgagcaagtc
     3601 tacccggccc gcgccgcgtc catcgtcaac cggcagcccc tgcggcgtcg gtcgacgatg
     3661 ttcccgacgc ggcgccgcat cgcgcgtgat ttccgaggcg gggcttggct ccgggggcac
     3721 gttcggctcg gccttcggcg gttcggcatt gagggaatgc atgccgctgc gcgacggcaa
     3781 gatcccgagc ccgctcagcc acccagccag ttcggcgctg tcgagcgaga tccggttttg
     3841 gtctgcgtaa gcctcgatgg ccgcgccgaa ctcacgcgcc gattgaaacc tctggcgtgg
     3901 ttcccgcgcc aaggcgtgac gaatgatttc cgccagcggc tcgggcacat gaccgccgtg
     3961 gcgctcgaac gtcgtcaaat cggcatcgtg gatgcgcgtg agcacctcga gctcgttttt
     4021 gcccgaaaac aaggggcaag taatcaacat ctcgcacagc aggatgccga ccgcgaacag
     4081 gtcactgcgc gggtccacct ccgccccgat cacctgctcc ggcgacatgt acccgagctt
     4141 tcccttgagt tcgcctggat tggtgcgccg atccacgagc gcgctgcgta cgatgccgaa
     4201 gtccgcgagc ttcacctcgc ccgcacgact aatcagcacg ttgccggggg acacatcgcg
     4261 gtgcaccagg cccaggggct gaccgtgctc gttggtcgct tcatgcgcga aggccaagcc
     4321 ccgcagcaca tcgcgcacga ccagcagcac gatcgaaatc gggaagcgct cccgcttgcc
     4381 cgccacgttg cggagcaatt tggcaacgga gacgccatcc acgaactcca tcgccatgaa
     4441 gagctcaccc tcgctctccc cgaaatcgac cacttgaacg atgttcgggt gctgcagggc
     4501 cgcgcagatg cgggcctcgt ccacgaacat cgcgaggaag cgcgggtcct tggccagctc
     4561 gggcaagatg cgtttaatcg cgaagcgctt ggcgaagcca tgcggccctg cacgatgtcc
     4621 aacgtagacc tccgccatcc cacccagccc aaggcgctcc ccgagcacgt agggtccgag
     4681 tttcatggcc gcgctcgcag cctccatgtg atcctgaagc ttagacgacg tcctttgcgg
     4741 tggccatgct ttcgactact ttcccccggg cagcctgacc gccgcccaat tcgagtgccc
     4801 acagctcgtt gttcctccca ccgttgttcc tctgggcgtt gttcctctcg gcgttgttcc
     4861 tctcgccgtt tgtacacacc gcgttgtaca tcgtggcgca ttccagcgtg ttttcgcatg
     4921 ggcggctcga attacttcct aatcgtttct tctaccctcg tttcttctac cctctgtttt
     4981 tggaacgcgt gtgagcactg aaactactcc ccctcccatc gagcggccga gctcgcccct
     5041 gagtcgccca ttgcaacggc aagccgtggg cgtgaccggc gcgtccactt ttttgggcgc
     5101 gaacttggtt gggatgctcg aagaagacga cgccgtacga cgcatcgtct gcctcgattc
     5161 caaagccccg agcaccgccg gcgctaaatc acgggcctac gacgtggatt tgacccagtc
     5221 ggcctccgag gaacggctga ccgagatcct gtccgccgaa gcagtggaca cgttggtgca
     5281 cttggctttc ctgccctccc cgacccacac cagcgctttt gcccacgaac tcgagagcgt
     5341 gggcacgatg cgtgcgctga atgcatgccg ccgcgccaac gtgcggaagt tcgtgatgtg
     5401 gagccagaca tttctgtacg gcgcgcatcc gacgaatccc aactacttgt ccgaaaagca
     5461 tcccctgcgc gcgccgcgcg gcgaagcgta cttccaagac aagctggacg cggagcggga
     5521 agtgcagagc tttggtcgcc cgggaaacgg gaaagttgcg accattctgc gcacgagccc
     5581 gatcctgggt cccaacgtgg atacattcct gacccggtac ttcagccgca agtgggtgcc
     5641 gacggtgctc ggtttcgatc cgctatggca gttcctgcac gaggccgacg cggttaccgc
     5701 gtttcgtcag gccgtgctgc gagatgcccc gggcacgttc aacgttacgg gtgacggggt
     5761 gctgccgctg cacacggcga ttcgcttgat tgggcggagc ccgctaccca tgcctcgcac
     5821 gttcacgcgc aacatggctt ctgcgttgtg ggtggcggag ctgtgcgaag cgcccccctc
     5881 cttctgtgac tttctgcaat atttgtgcgt cgctgacggt gcgctcgcca agcgcgcatt
     5941 gggcttcgag cccatgtata cgagccgaga agccgtaatc gagttcggca atgcccaacg
     6001 cctgcgcgac gtccgcctgc tttcggagcg ccccgcttga accaccgacg caaacccccc
     6061 cgcccctacg atccccgttc gcccaaaaag aagccgcggc agcaggcacg cccgggtcat
     6121 tccccgcgtg gcgaagggga acgccacaag ccacgccccg agtacccagc tcgggacgct
     6181 cgtagggagc ctccgcgcga accacgtccg gggctcgaca tgtcgcgccc gccggtgcgg
     6241 aagccccgcc cggaccgcgc gacgcctccg ctcggaacgg ggcatcggtc acctttcaat
     6301 cctcccgtcg agcggcccca tgatcggggg cgcgagcgag acgaagagcc gcgagcatat
     6361 cctcctcgag cggccgacca cgcgattcac gacgaagcgc ccagcagcga gtggcaggat
     6421 caattttccg actatctgga cgaggaagtt ccggagctcg gcgtgggctc acgcatggtc
     6481 cctccggaag tacgctacga agagaagccc gagcccgcgc ccgttcccaa gcccctcacg
     6541 ccggtggact tcgaagcgca aattcgaacg ctggaagcac gcttggatgg cttgattcgt
     6601 cgcgccacgc tggccgagga gagcgaagcc gaagccgcgc cggcatcttt ccgcagcccc
     6661 ggccctcaag tcgaagccac gcccgccg
//
LOCUS       DASTKU010000039         6033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1345269, whole genome shotgun sequence.
ACCESSION   DASTKU010000039 DASTKU010000000
VERSION     DASTKU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345269"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VFQ61_01310"
     CDS             complement(<1..768)
                     /locus_tag="VFQ61_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9953106.1"
                     /translation="MKRTDRTELAQNRSNSRRNLLLAGAAMMTGACAPGALSSNQPVH
                     QFSTVDTSRKGKRHMGTITTQDGTQLYYKDWGSGRPIVFSHGWPLSSDSWESQMLFLA
                     EHGFRCIAHDRRGHGRSSQPWSGNDMNTYADDLARVIESLNLEQVTLVGFSTGGGEVT
                     RYIGRHGTKRVRQAVLVSAIPPLMLKTPHNPAGTPKEVFDGLRAASIADRSKLYRDLA
                     SGPFFGFNRPGAQVSQGMIDSFWLQGMQSGHKNAYDCI"
     gene            870..1493
                     /locus_tag="VFQ61_01315"
     CDS             870..1493
                     /locus_tag="VFQ61_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9953107.1"
                     /translation="MLLQNDPGVLRSDEDYVTGLKSPSDSESHEAVVELRAFLRGALA
                     RSFGRQLSDSDLDDITQESLLRVCARLDGFQGQARFTTWATTIAVHCALSELRRRRFK
                     LVSLEDAAQQARAELTQEAELISGDQEAELAKLQRGIHEALTERQREATLAKLGGLPL
                     MEIARRLGTTQGAVYKLLHDARKRLKSYLESTERPNEELVRTPVGAA"
     gene            1490..1714
                     /locus_tag="VFQ61_01320"
     CDS             1490..1714
                     /locus_tag="VFQ61_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953108.1"
                     /translation="MSKRPEKHLTLRSWLEVTQDEEVDCDRFGELLAQWIDGRIEDPR
                     LVALLEHHRRLCHECDEGAALVAAAVGAQS"
     gene            complement(1754..2635)
                     /locus_tag="VFQ61_01325"
     CDS             complement(1754..2635)
                     /locus_tag="VFQ61_01325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9953109.1"
                     /translation="MMKPQSTSGSSPRVASRRNVIKHTLSGGFALAVGPIASTALATP
                     SDGLQAGEVKIPTNNGEIPAYRAGPQNLKRKAPVLLVVHEIFGVHEHIKDVCRRFAKL
                     GYFAIAPELFARQGDPSTISDIKELQQKIVSKASDEQVMSDLDASVAWAKQQPHVDTH
                     KLAITGFCWGGRITWLYAAHQPNLRAGVAWYGRLTGDRDPLHPKFPIDLCADLKAPVL
                     GLYGGKDQGIPVETVEQMRTALQQTNVHAAKKSTIKVYPDSGHAFFADYRPSYNKVDA
                     EDGFKRCVQWLKQHGVT"
     gene            complement(2851..3294)
                     /locus_tag="VFQ61_01330"
     CDS             complement(2851..3294)
                     /locus_tag="VFQ61_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9953110.1"
                     /translation="MQIQKLTATDVLLARALFPMMAQVFSEPCDELSDAYLQGLLGRE
                     SFWAFAALAEEQVVGGLTGYLLPMTRRETHELFIYDVAVREDYQRRGVGRALMRAAIQ
                     AANGIGAQNAFVPADAEDDHALRFYRALGGTAAESTIFTFPNVTP"
     gene            complement(3332..4012)
                     /locus_tag="VFQ61_01335"
     CDS             complement(3332..4012)
                     /locus_tag="VFQ61_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HET9953111.1"
                     /translation="MTLAPTAEARVFRAPDGKLLTPPAGWACLPPGDAGLTRRVKAAG
                     PSWAVVEKKGRKTFSQGVWAPAENIAAARAALDEERATPAYAKKRAADVQRREVREQR
                     YAEDFELEVLRFLRFSERYEAMARKLARLVSAHATVVGSGTVARTQRISVERRAEAAV
                     IAWMRHQTTEYDNLSIPRIKGKRREVRRDLAQISRSLLSLHRGEVGHAEAACPLCSAL
                     AKSPAPSA"
     gene            complement(4626..>6033)
                     /locus_tag="VFQ61_01340"
     CDS             complement(4626..>6033)
                     /locus_tag="VFQ61_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9953112.1"
                     /translation="RWFPSVESGEITAATLAGKTLEKLSPYASKLLFPRGLAMFPRGT
                     INGYFDPHDQGMGSKLTCASIDPAGSHWSTGRSLDHVAAELVNPGTKSPLVLSVGSAF
                     ANVKGILSYSAPNTPYSPETKPANVYKSLTNVLAAGKGGTTTPTTPAPVTEADYRIKQ
                     GKSILDLVKADLDSFRRLKMSKADLKKVDDWASLLRQTEVAVTGTGSTMAAAVSAACN
                     GDTAAALGVSDATLKEAAGAGGGASLGVAFTKGGDMMFNLIALSMMCDTNRVIVMQWP
                     GFVTFKWDGIEHTYDHHGLSHRNGSAAVGGTCVTGVIEMINQIDQWYAGRFAKLVGLI
                     DSIKEGDRTMLDNSAVMWLPELADGNAHNNNNLPIVIAGSAGGYFKTGVSVNLDPKGK
                     ALGTGNSEASCASGTDVGFNTSSNGGNVPLNKLYVTLLNALGAKQDGMPITQFGAMDT
                     NDVTKGITDPGELTALKA"
BASE COUNT         1119 a         1837 c         1914 g         1163 t
ORIGIN      
        1 gatgcagtcg taggcgttct tgtgccccga ctgcatgcct tgcaaccaga atgaatcaat
       61 cattccttgc gatacctggg ctcccgggcg attgaagccg aagaatgggc cgctcgccaa
      121 atcacgatac agcttggatc gatcggcgat cgaggcagcc cgcaacccgt cgaacacctc
      181 cttgggtgtt ccggcggggt tgtggggtgt tttgagcatg agcggcggaa tcgccgagac
      241 caagaccgcc tgccgcactc gcttggtgcc gtggcgccca atgtaacgag tcacttcacc
      301 gccgcccgtc gagaagccga ccaaggtgac ctgttcgaga ttcaatgact cgatgacccg
      361 ggccaaatcg tcggcataag tgttcatgtc attgccagac cagggttgac tcgatcgccc
      421 gtggccccgc cgatcatggg caatacagcg gaagccgtgc tcggccaaga acagcatctg
      481 agattcccag ctgtccgagc tgagcggcca accgtgacta aatacgatcg ggcgtccgct
      541 gccccaatct ttgtaataga gctgagtgcc gtcttgagtg gtgatcgtgc ccatgtgtct
      601 tttacccttt cgagaggtgt ccaccgtcga gaactgatga actggttggt tgctcgagag
      661 cgccccgggc gcgcacgcgc cggtcatcat cgccgctccg gccagcagca ggttgcgacg
      721 agagttcgag cggttttgcg cgagttcggt gcggtccgtg cgtttcatga ccgtgagacg
      781 caccggcact ggggatctta cctggccggc tcgttctttt ttcgaagcgc ttggttttcc
      841 cgaggcccgc gtgctcgctt gaccccggca tgcttctaca gaatgatccc ggcgtgctgc
      901 gcagcgatga ggactacgtc acagggctga aatcgccctc ggactccgaa agccatgagg
      961 ccgtagtcga gcttcgcgct ttcttacggg gagctttggc gcgcagcttc ggacggcagc
     1021 tgagcgacag cgatctagac gacattactc aggagagcct gttgcgggtg tgcgcgcggc
     1081 tggacggttt tcaagggcag gcccggttca cgacgtgggc gaccacgatt gcggtccact
     1141 gcgctctctc cgaactgcga cgtcggcgtt tcaaactcgt aagcttggaa gacgcggcgc
     1201 agcaggcgcg agccgagctg acccaagaag cagagctcat ttcgggtgac caagaagccg
     1261 agctcgcgaa gctacagcgc ggcattcatg aagcgctcac ggagcgccag cgtgaggcca
     1321 ctctcgccaa actcggcggc ctcccactca tggaaattgc ccgccggctc ggcaccaccc
     1381 agggcgccgt ctacaagctc ttgcacgacg cgcgcaagcg cctgaagagt tacctcgagt
     1441 caacggaacg gccgaacgaa gaactggttc gaaccccagt aggtgcagca tgagcaaaag
     1501 accggagaag cacctgaccc tgcgaagctg gctcgaggtc acgcaggacg aggaggtaga
     1561 ctgcgatcga ttcggggaac tgctcgccca atggatcgat ggtcgaatcg aggatccgcg
     1621 cctcgtcgcg ctgctcgagc atcatcgccg cttgtgccac gaatgcgacg aaggagcggc
     1681 cctcgtcgca gccgccgtcg gcgcgcagag ctgaagcgta ctggcctcag agcttggggg
     1741 cgtcacccga ccttcaagtg accccgtgtt gcttcaacca ctgcacgcaa cgcttgaagc
     1801 catcctcggc gtcaaccttg ttgtagctcg gtcggtagtc cgcgaagaag gcgtgacctg
     1861 aatcgggata gactttgatt gtggacttct tggccgcgtg cacgttcgtt tgctgcaacg
     1921 cggtccgcat ctgttccacg gtctccaccg ggatcccttg atccttacct ccgtagagcc
     1981 ccagcaccgg agccttcaaa tccgcgcaga gatcgatcgg gaacttggga tgcagcgggt
     2041 cgcgatcgcc tgtcagccga ccataccacg cgacccccgc ccgtaagttt ggttggtgcg
     2101 cagcatacaa ccaagtaatt cggccgcccc aacagaaccc cgtaatcgcg agtttatgtg
     2161 tatcgacatg gggttgttgt ttggcccaag caacacttgc gtccaaatcg gacatcactt
     2221 gttcgtccga ggctttggag acgatctttt gttgcagctc tttgatatcc gagatcgtag
     2281 acgggtcgcc ctgccgcgcg aatagctcgg gcgcaatcgc gaaataaccg agcttcgcga
     2341 agcgacggca gacgtccttg atatgctcgt gcaccccgaa gatttcatgc acgaccagta
     2401 gcaccggcgc cttccgcttc agattctgcg gccccgctcg gtaagcgggg atctctccgt
     2461 tgttagtggg gatcttgacc tcgcccgcct gcaacccatc actcggcgtg gccaacgccg
     2521 tgctcgcgat aggccccacg gcaagcgcaa acccgccgct gagcgtgtgt ttgatcacat
     2581 tccggcgcga agcaactcga gggctcgacc cagacgtgga ttgcggcttc atcatggttg
     2641 ctatcctaag aaaacggcag cgagattaga gaaccaaata attcagaaag accgagacgc
     2701 agttcgggcc ctcaacccta ctgcatcgtc acgaactggg ggcgcgagaa acgttggtct
     2761 cgaacgcgcc cggagcgtac gtttatgccc caatggcggc cccatgcggg gctcttcacc
     2821 caggcttaga ccgtagtgct aacgaggtgc tcacggggtc acattcggga acgtaaagat
     2881 cgtcgactct gccgcggttc cgccgagcgc acgataaaac cgcagcgcgt gatcatcctc
     2941 cgcatcagcg ggcacgaatg cattctgagc gccaatccca ttcgcggcct ggatcgccgc
     3001 gcgcatcaaa gcccgcccca cgccgcggcg ttggtaatcc tcacgcaccg ccacatcgta
     3061 gatgaacagc tcgtgcgtct cgcgccgagt catcgggagc aggtagccag tcagcccacc
     3121 cacgacctgc tcctcagcca acgcggcaaa cgcccaaaaa ctctcgcgcc cgagcaagcc
     3181 ctgtagatag gcgtcactca gctcgtcaca gggctcggag aacacctgcg ccatcatcgg
     3241 gaacaatgca cgcgccaaca gcacgtctgt cgcggtgagc ttctggattt gcatgggatt
     3301 gggagcctac cccaatcgga agatcttggg gtcaagcgga cggcgctggg ctctttgcga
     3361 gcgcggaaca gagagggcag gccgcttccg cgtgcccgac ctccccgcga tgcagcgaca
     3421 acaggcttcg cgaaatctga gcgagatcgc ggcgcacttc gcggcgctta cctttgattc
     3481 gcgggattga aagattgtca tactcggtcg tttgatgccg catccaggcg atcacggcgg
     3541 cttccgccct gcgctccacc gaaatgcgct gtgtacgggc caccgtaccg ctgcccacca
     3601 ccgtcgcgtg cgcgctcacg agccgcgcga gcttgcgagc catggcttcg tagcgctcgc
     3661 tgaatcgcag aaagcgcaac acctccagct cgaaatcctc ggcgtagcgt tgctctcgaa
     3721 cctctcggcg ctgcacgtct gcggcgcgct tctttgcgta ggcgggggtg gcgcgctcct
     3781 catccagggc cgcccgcgct gccgcgatat tctccgctgg cgcccaaaca ccttgcgaaa
     3841 acgttttcct ccccttcttt tcgaccaccg cccaagaggg ccccgccgcc ttcactcgcc
     3901 gagtcagccc cgcgtccccg ggaggcaaac acgcccaacc cgccggaggc gtaagcagct
     3961 taccatctgg cgcccggaac acgcgcgcct ccgccgtggg agcgagagtc aaagaagcag
     4021 tattcgagct ggcaagtgcg gtgggcgtcg agggcgcggg catctgcgtc ctagctagtg
     4081 gctcacgtgc ggagaaacaa gagccagttg ccgagacaac ggtcgaaccg cggcaaacgg
     4141 cggacctcca ccccctccgc ggctccgagg cgccacgtcg gcagtctgga attgcagatt
     4201 aaggtctcgc ccaattgagg ccttgggaaa agatgagagc cgtctagagc tgcagacaca
     4261 gcgggctcaa cccagtcttc aagtgcttcg aatcaggggc cctcgactca cgcgggcgcc
     4321 caggacgttg gttattgcgg gggcatgcgc ggtgttgggg gaggatgcga gggtagcgac
     4381 tgcggagttg ggatcatggg actcgcgtcg cgcccgatac agcgcggcca atcctctctt
     4441 gtatcctcaa ggcttgctca cacggtggct gactccaacc cggtggccgc agggagacgt
     4501 tgggtgcgtc gcgacaactg gcggccggag agtgctttcg gactggacgg agagctctcc
     4561 cacgccggcg cgggagaccg aattcgcgga gaaagggaga gctctctgtt tcgcggcgcg
     4621 tttcgtcagg cttttagggc ggtgagttcg ccggggtcgg tgatgccttt ggtgacgtcg
     4681 ttggtatcca tggcgccgaa ttgggtgatg ggcattccgt cttgtttggc gccaagggcg
     4741 ttgaggaggg tgacgtagag tttgttgagg gggacgttac cgccgttgga ggaggtgttg
     4801 aagccaacgt cggtgccgct ggcgcagctg gcttcgctgt tgccggtgcc aagggctttg
     4861 cctttgggat cgagattgac ggagacgccg gtcttgaagt agccgcccgc gctgccggcg
     4921 atgacgatgg ggaggttgtt gttgttgtgg gcgttaccgt cggcaagttc gggcagccac
     4981 atgacggcgg agttgtcgag catggtgcgg tcgccttctt tgatgctgtc gatcaagccc
     5041 acgagcttgg cgaagcgacc cgcataccac tgatcgattt gattgatcat ctcgatcacg
     5101 ccagtcacgc aggtgccgcc cacggccgcg ctgccgttgc ggtgagaaag gccgtggtga
     5161 tcataggtgt gttcgatgcc gtcccacttg aaggtgacga agccaggcca ctgcatgacg
     5221 atgacccggt tggtgtcgca catcatgctg agggcgatta ggttgaacat catgtcgccg
     5281 cctttcgtga aggcgacgcc gaggctggcc cctcccccgg ccccggcggc ttctttgagg
     5341 gtggcgtcgg agacgcctag cgccgcggcc gtgtcgccgt tgcacgcggc ggagacggcg
     5401 gcggccatgg tggagccggt gccggtgacc gcgacttcgg tttgacgcag caatgaggcc
     5461 cagtcgtcga ccttcttcag gtccgctttg ctcatcttga gccgccggaa tgagtccaag
     5521 tcggctttga ccaaatcgag gatgctcttg ccctgtttga ttcggtagtc cgcctcggtg
     5581 acgggggcgg gggtggtggg agtggtggtt ccacctttgc ccgcggcaag cacgttggtc
     5641 agtgacttgt agacgttggc gggcttcgtc tcgggcgagt atggcgtgtt gggcgcgctg
     5701 taggacagga tgcctttcac gttcgcgaac gcactcccca cggagagcac cagtgggctc
     5761 ttggtgccgg ggttgacgag ctcggccgca acgtggtcga gcgagcgccc cgtggaccaa
     5821 tggctgcccg ccggatcgat cgaggcgcag gtgagcttcg agcccatgcc ctgatcgtgc
     5881 ggatcgaaat agccattgat ggtgccgcgc gggaacatcg cgagaccacg cgggaatagg
     5941 agcttggagg cgtagggact cagcttctcg agggttttgc cagccagagt agcggcggtg
     6001 atttcgccgc tctccacgga tggaaaccag cga
//
LOCUS       DASTKU010000040         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_898796, whole genome shotgun sequence.
ACCESSION   DASTKU010000040 DASTKU010000000
VERSION     DASTKU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898796"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFQ61_01345"
     CDS             <1..234
                     /locus_tag="VFQ61_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET9953113.1"
                     /translation="LLEQLLYGMPFTQLDQSQVDYACWCDELRVMSALATLPRNDIEH
                     LLSTDEVLEIACEYCKRDYQIAPARLRGLLEKS"
     gene            240..2075
                     /locus_tag="VFQ61_01350"
     CDS             240..2075
                     /locus_tag="VFQ61_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HET9953114.1"
                     /translation="MRAASPRIGVRWGGWLALAVGAGCQGNPQTTRTLAPAPTHVASS
                     TDGAAPRVTNQPGEFVLSVMSLNDLHGKLGALPWFAGYVRAVAARRVQDGAVIVVDAG
                     DMFQGTLGSNATQGSAVVEAYNALPLTAAALGNHEFDYGYLSSAGATAPLLGADPQGA
                     LRARIAEAQFKVLSANLVSQATGRLPEFKNLAADALIEVRGVQVGLVGILTEETPRIV
                     RRDYFAGLAVKQAAPSVIERARKLRERGAEVVLVLAHAGGDCSRFDQACDLSSCDAES
                     GEIFRLARALPPGLVDGIVAGHTHAGVAHVENGIPIVEAYSRGKAFARLDIRLTVRAG
                     RPNPDGTPAKPVRHVSEAHPFPPEPLCPDSAELAPCSGHLYEGQMIEPDPELERVIGP
                     ALERAKAARAQWLGVELTSAAAGEHGRESALGNLFVDALREAHPEFDVAIINGGSLRA
                     DLPAGPLSYGTLYEAMPFDNGLVQLTLTGAELRRLFTQHLSHDQHGIVSISGLRLGAS
                     CAAHGLVIELKRESGSRVRDTESLRILTSDYLATGGDQLLTPLALSEGRIQPLGNELM
                     RDALARGLARRPRRDPQSVRVIDPKHPRLQLPSPRPVRCSGSAGP"
     gene            complement(2090..>2372)
                     /locus_tag="VFQ61_01355"
     CDS             complement(2090..>2372)
                     /locus_tag="VFQ61_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953115.1"
                     /translation="GPAFDAANRAYELGFGRKLQTVGIGGSIPFVALFGRRYAELPLI
                     LNGVLDPESNAHGPDESLHLGVFEKAILTNIHLLDELSRAPLRPPHGSA"
BASE COUNT          418 a          793 c          772 g          389 t
ORIGIN      
        1 ctgctcgagc agctgctcta cggcatgccc ttcacgcagc tggaccagag ccaggtggac
       61 tacgcctgct ggtgcgacga gctgagggtc atgtcggctc tcgccacttt accccgcaac
      121 gacatcgagc atctgttgtc gacagacgag gtgctcgaga ttgcgtgcga gtactgcaag
      181 cgtgactacc agattgcgcc cgctcgcctg cgcggcctac tcgagaagag ctgacgcgaa
      241 tgcgcgcagc ttcgccgagg atcggggtgc gctggggcgg ttggctcgcg ctggccgtcg
      301 gtgcgggctg ccagggcaac ccgcaaacca cacgaacact ggcccccgcc cccacccacg
      361 tcgcctcgag tactgacggc gccgcgcctc gcgttacgaa tcagcccggc gagttcgtgc
      421 tgagcgtgat gagtctgaac gacctgcacg gcaagctcgg ggccttgcct tggttcgccg
      481 gctacgtgcg ggccgtagcg gcgcgccgtg tgcaagacgg ggccgtgatc gtggtggacg
      541 ccggggatat gtttcaaggc acattgggtt ccaacgcgac ccaaggcagc gcggtagtgg
      601 aagcgtacaa cgcgctcccg ctgacggcag cggcgctagg aaaccacgag ttcgactacg
      661 ggtacctatc atccgcggga gcaacggccc cgcttctggg agccgatcca caaggcgcgt
      721 tgcgcgcccg catcgccgaa gcgcagttca aggtgctgag cgccaacctg gtcagccaag
      781 ccactggacg cctgccagaa ttcaagaacc tcgcggctga cgcgctcatc gaagtacggg
      841 gcgtgcaggt cggtttggtc ggaattctaa ctgaagaaac cccacgcatc gtgcgccgcg
      901 actacttcgc tggcttggcc gtaaagcagg ccgcgccgag cgtgatcgag cgtgcgcgca
      961 agttgcgcga aaggggcgcc gaagtagtgc tagtgctggc gcacgcaggc ggagattgct
     1021 cgcgcttcga tcaggcctgc gatctgtcga gctgcgacgc cgagagcggg gagatttttc
     1081 gcttggcgcg agcgctccct ccagggctcg tggacggcat cgtggccggg catacccacg
     1141 ccggggtagc gcacgtcgag aacgggattc caatcgtgga ggcctattca cgtggcaaag
     1201 ccttcgcgcg gctcgacatc cgcctaacgg tacgcgccgg gcgtccgaac ccggacggca
     1261 ctcccgcgaa gcccgttcgc cacgtcagcg aggcccatcc cttcccgccc gagccgctat
     1321 gccccgattc tgcagagctc gcgccgtgca gcggtcacct gtacgaagga caaatgatcg
     1381 agcccgatcc ggagctggag cgcgtgattg ggcccgctct ggagcgcgca aaggctgccc
     1441 gggcgcagtg gctgggcgtc gagctcacgt ctgcagcagc cggagaacac ggacgtgaat
     1501 cggcgctcgg caacttgttc gtcgatgccc tgcgtgaagc gcacccggag ttcgacgtcg
     1561 cgatcatcaa cggaggctcg ctgcgcgcag acctgccagc ggggccactc tcctatggga
     1621 cactctacga agcgatgcca ttcgacaatg gcctcgtgca gctcacgctc accggcgccg
     1681 agttgcggcg gctcttcaca cagcacttgt cccacgatca gcacggcatc gtgtcaatct
     1741 cgggcctgcg gctcggcgcg tcttgtgcgg cccacggcct cgtgatcgag ctcaagcgcg
     1801 agagcggcag ccgcgtgcga gacaccgagt cactccgcat cttgactagc gactacttgg
     1861 ccaccggagg cgaccaactc ctcacgccgc tcgctctgag cgaaggccgc atccagccgc
     1921 tgggcaacga gctcatgcga gacgcgctgg cgcgcggtct cgcacggcgg ccgcgccggg
     1981 acccgcagtc cgtccgcgtc atcgatccca agcatccccg actgcaactc ccgagcccgc
     2041 ggcccgttcg ttgttcgggc agcgccggcc cctgaggcct ttcgagctcc taagcgcttc
     2101 cgtgtggcgg tctcagcggg gcgcgcgaca gctcgtcgag caagtggata ttggtgagga
     2161 tggccttttc gaagacgccc aaatgcaacg actcgtcggg gccgtgagcg ttgctctccg
     2221 ggtcgagcac cccattcaag atgagaggta gctccgcata gcgcctgccg aacaaagcca
     2281 cgaacggaat cgagccaccg attccgacgg tctgcagctt gcgaccaaag cccagttcgt
     2341 aagctcgatt tgccgcgtcg aaagctggcc cc
//
LOCUS       DASTKU010000041         5065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1568474, whole genome shotgun sequence.
ACCESSION   DASTKU010000041 DASTKU010000000
VERSION     DASTKU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568474"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFQ61_01360"
     CDS             <1..243
                     /locus_tag="VFQ61_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953116.1"
                     /translation="LMDVLTPKALGSLVALYEHKVFVQGSIWGINSFDQWGVELGKVL
                     AKRVAPELSAAAEPALAHDSSTNTLIRRYRARRNAG"
     gene            complement(246..665)
                     /locus_tag="VFQ61_01365"
     CDS             complement(246..665)
                     /locus_tag="VFQ61_01365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET9953117.1"
                     /translation="MAESIFSKILRKEIPCHRVYEDAHVLAFLDINPIAYGHTLVIPK
                     EPAETLDQLSEEASAAIGRVLPRICRAVQSVTGVRAYNVLQNNGSEAHQAVMHVHFHV
                     IPKPNEGQGLGIRWPAGKLDADAGKELASKVQAALEG"
     gene            706..1605
                     /locus_tag="VFQ61_01370"
     CDS             706..1605
                     /locus_tag="VFQ61_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9953118.1"
                     /translation="MLKLSSVLGNSQRLDGGAMFGNAPRALWSRWLAPDEAGRVPLAC
                     RSFLLETEQHKLLIETGIGSFFEPGLKDRYGVVESQHVLIQSLAHLGVRPNEVDWVVL
                     SHLHFDHAGGLLSEYEPGHALRLVFPKARILVSRGAFERARAPHPRDRASFIPGLVEL
                     LQESGRLVLWDEQSRLLPEELGRALAFELSHGHTPDMLHTWLRGTRGSAFFCADLVPG
                     AAWVHLPITMGYDRYPELLIDEKTRLYTELLERGTQLLFTHDPSVAAARLQLDARGRY
                     AAADPVAEFVGWDLDASPAPAPG"
     gene            complement(1639..3282)
                     /gene="pgm"
                     /locus_tag="VFQ61_01375"
     CDS             complement(1639..3282)
                     /gene="pgm"
                     /locus_tag="VFQ61_01375"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HET9953119.1"
                     /translation="MTAHPLAGKPAPSEVLIDPEALISRYYSERPDPAVAAERVQFGT
                     SGHRGSPLQRAFNEAHILATTQAICEYRKSVGTDGPLFLGIDSHAVSEPAGRSALEVL
                     AANGVEVRISEGARFTPTPALSRAILAYNEGRKAGLSDGIVVTPSHNPPEDGGFKYNP
                     PHGGPADTNVTQWVENRANQLLAQNNRGVLRVSYEKAIAASSTRPYDFLGEYVEALGT
                     VIDMDAIRASGLRLGADPLGGASVEYWPRIAERYGLNLRVVNDRVDPRFAFMTLDHDG
                     KIRMDCSSAYAMQSLVALKNEYDVAFGNDTDADRHGIVTPSLGLMNPNHYLAVAIHYL
                     YQNRPEWPKQARVGKTLVSSSLIDRVAREIGREVAEVPVGFKWFVDGLIHGSLAFGGE
                     ESAGASFLRRDGRVWTTDKDGLILCLLAGEIAAKTGKEPGLHYRELVARLGTPYYRRI
                     DVPATPAQKNVLKRLSASSVSASTLASEPIRACLTEAPAGGSIGGLKVVSDNGWFAAR
                     PSGTENVYKVYAESFRSEAALEAIISEAQAIVAQALSAS"
     gene            3618..3962
                     /locus_tag="VFQ61_01380"
     CDS             3618..3962
                     /locus_tag="VFQ61_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9953120.1"
                     /translation="MKSPSEIQRLAKLLGGVPILGIFPGSPAEQVGLMYGDIVLSVNG
                     HDTKSFEDFLRAHTGKETSVKLQIFRDGRNLTFEISFRQEDWVEAEDDLEEPELLSPM
                     AYASPTRASGLC"
     gene            complement(3985..4389)
                     /locus_tag="VFQ61_01385"
     CDS             complement(3985..4389)
                     /locus_tag="VFQ61_01385"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET9953121.1"
                     /translation="MFTVSVRGHMMIAHSFRGELFGPAQRLHGATYVVDVEFRRKNLS
                     ADGVVVDIGRATDVLNSLLGELNFQNLDEMPAFSGVNTTTEFLAFTLFERFADRCAKG
                     ELGPGSEQLESMKVTLHESHVAWACYEAPLTR"
     gene            complement(4515..>5065)
                     /locus_tag="VFQ61_01390"
     CDS             complement(4515..>5065)
                     /locus_tag="VFQ61_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953122.1"
                     /translation="PRLGDRVSVVGAGVLGSLLAFLLSRNLQTEVELIDVLPERRRVA
                     DAFGVRFSTPERAERDRDLVFHASGRPAGLAAALEIAGAETTIVELSWYGDAEVAAPL
                     GQRFHSARLTLRSSQVGRLSPNARARFDMRKRLELALALCADSALDVLIDGECTFDEL
                     PAVMAEITAPGARALCQRVRYF"
BASE COUNT          909 a         1669 c         1576 g          911 t
ORIGIN      
        1 ttgatggacg tgctcacgcc aaaagcactg ggttcactgg ttgcgcttta cgagcacaag
       61 gtgttcgtgc agggcagcat ctggggaatc aactcgttcg atcagtgggg tgtcgagctc
      121 ggcaaggtgc tcgccaagcg tgtggcgccc gagctcagcg ccgcggccga gcctgcgctc
      181 gcgcacgata gctcgaccaa caccttgatc cgccgctacc gcgcgcggcg caacgccggc
      241 tgagttcagc cctcgagcgc ggcctgcacc ttcgaggcaa gctctttgcc ggcatcggcg
      301 tcgagcttgc ccgcaggcca ccgaatgccg agcccctgcc cctcgttggg cttgggaatg
      361 acgtggaagt ggacgtgcat gaccgcctga tgcgcctcgc tgccgttgtt ctgcagcacg
      421 ttgtaagcgc gcaccccggt gacgctttgt acggcccggc aaatccgcgg caggacgcgc
      481 ccaatggcgg ccgaagcttc ttcggagagc tgatccagag tctcggcggg ctctttggga
      541 atcaccagcg tgtgcccgta ggcaatcggg ttgatatcca agaaagcgag cacgtgcgcg
      601 tcctcgtaaa cgcggtggca cgggatttct ttgcgcaaga tcttgctgaa gatcgattcg
      661 gccataggct gcttttaaca ccagctccgc ggcggagaaa acaacatgct caagctcagc
      721 tctgtgctcg ggaactcgca acgattggac ggcggagcca tgttcggcaa cgccccccgc
      781 gcgctgtgga gccgttggct agctccggac gaggcggggc gagtgccgct ggcttgccgc
      841 tccttcttgc tcgagaccga gcagcacaag ctgctgatcg agaccggaat aggctctttc
      901 ttcgaaccag gtctcaagga tcgttatggc gtggtcgaga gccagcatgt gttgatccaa
      961 tcactggcac acctcggcgt acggccgaac gaggtcgatt gggtggtgct cagccatttg
     1021 cacttcgatc atgcgggggg tttgttatcc gagtacgagc cgggccacgc ccttcgactc
     1081 gtcttcccga aggcgcgtat tctggtcagc cgcggcgcct tcgagcgggc tcgcgcgccg
     1141 catccgcgcg accgggcctc cttcatccct gggctcgtgg agctactgca ggagagcggt
     1201 cgactggtgc tgtgggacga acagagccgg ctgttacccg aggagctcgg gcgcgccctg
     1261 gccttcgagc tctcccatgg ccacaccccg gacatgctcc acacgtggct gcggggcacc
     1321 cgcggatccg cgttcttctg cgcggatttg gtgcccggcg cagcctgggt gcacttgccg
     1381 atcacgatgg gctacgaccg ctaccccgag ctgctcatcg acgagaagac gcggctctac
     1441 accgagctcc tcgagcgcgg tacccagctg ctcttcacgc atgacccgag cgtcgctgcc
     1501 gcgcggctcc agttggacgc gcgcggccgc tacgccgcag ccgatccggt ggcagaattc
     1561 gtgggctggg acctggacgc gagccctgca cccgcgcccg gctgagttgc tgcgatccgg
     1621 tcagagactc cgtgattctc agctcgcgga cagcgcctga gcgacgatgg cctgcgcctc
     1681 ggagatgatg gcttcgagcg ccgcttcgct acggaagctt tccgcatata ccttgtagac
     1741 attctcggtg ccggaggggc gcgccgcgaa ccagccgttg tcggagacga ccttcaggcc
     1801 gccaatcgag ccgcccgcgg gtgcctcggt caaacacgca cggattggtt ccgacgcgag
     1861 cgtcgatgcc gacacggagc tcgcggacaa gcgcttcaat acgttcttct gcgccggggt
     1921 cgcgggtacg tcgatacgcc ggtagtacgg ggtccccaag cgggccacga gctcacggta
     1981 atgcagcccc ggctccttgc ccgtcttggc ggctatctcg cccgcgagca gacacagaat
     2041 caggccgtcc ttgtcggtcg tccacacccg cccgtctcgc cggaggaagc tcgcgcctgc
     2101 gctctcctcc ccgccgaacg ccaagctgcc gtggatgagc ccatcgacga accacttgaa
     2161 accgacgggg acttccgcga cctcacgtcc aatctcgcgc gccacccgat cgatcaggct
     2221 gctgctcacc agagttttcc cgacccgggc ctgtttcggc cattcgggtc ggttttggta
     2281 gaggtagtgg atcgccaccg ccaggtagtg attcggattc atcaagccca gcgagggcgt
     2341 gacgatgccg tgacgatcgg cgtccgtgtc gttgccgaag gccacgtcgt actcattctt
     2401 cagcgcgacc aggctctgca tcgcatacgc gctcgagcag tccatccgga tcttgccgtc
     2461 gtgatccaag gtcatgaatg caaaacgcgg atcgacgcgg tcgttgacga cccgcaaatt
     2521 caaaccatag cgctccgcga tccgcggcca gtattcgacg ctcgcgccgc ccagcggatc
     2581 cgccccgagg cgcaagcccg acgcgcgaat cgcgtccata tcgatcaccg tgcctagcgc
     2641 ctccacgtac tcaccgagga agtcgtaagg tcgcgtcgag gaagccgcga ttgctttttc
     2701 gtagctgacg cgcagcacgc ctcgattgtt ttgcgcgagc agctggttgg ctcgattttc
     2761 gacccattgc gtgacgttgg tgtcagcggg gccaccgtgc ggcgggttgt atttgaagcc
     2821 gccgtcctcc ggcggattgt gcgacggagt caccacgatg ccgtcgctca agcccgcctt
     2881 gcggccttcg ttgtaggcga ggatggcgcg cgatagcgcc ggcgtcggcg tgaagcgcgc
     2941 cccctccgag atgcgtactt ccacgccgtt ggccgcaagc acctcgagcg cgctacgccc
     3001 cgcgggctcg gagacggcgt gcgagtcgat gccgagaaat agcggaccgt ccgtaccgac
     3061 cgacttacgg tactcgcaga tggcctgcgt cgtagccaag atgtgcgctt cgttgaacgc
     3121 gcgctgcaac ggtgaaccac ggtggccact cgttccgaac tgaacgcgct cggccgcgac
     3181 ggccgggtca ggtcgttccg agtagtagcg gctgatcaac gcttcggggt cgatcagaac
     3241 ttcagacggt gcgggttttc cggcgagagg gtgggcagtc atggcactaa ggcccgcacc
     3301 atacctgcga acgcgcgcgg cggttagtgt gctctcacgc gcacctcgga gcgcggctcg
     3361 ggccgggttt cggtgggttt tctcgcgctg tcccaagccg agtcgagccc gatcccgctc
     3421 agagacgatc cgagccgaac cagcgagctc gagctcggtt caactcgggc tttcccggga
     3481 ttttcgcgcc gaactgcggc tggcatgttt cgtggaaaag cagaacgcac aactgacggg
     3541 ccgcagctca aacctgacgg ccagctaatc ccgccgctac ccgactcggt agcggcgccc
     3601 gaaagagagc cccccacatg aagagcccct ccgaaatcca acgcctcgca aagctgctgg
     3661 gcggcgttcc catcctcggc attttccccg gcagtcctgc cgagcaagtc gggctcatgt
     3721 acggtgacat cgtgctttcg gtgaacggcc acgacaccaa gagcttcgag gacttcctga
     3781 gagctcatac tggcaaggaa acctccgtga agctccaaat ctttcgcgat ggccggaacc
     3841 tgaccttcga gatctcgttc cgccaggaag attgggtgga agccgaagac gatctcgagg
     3901 agcccgagtt gttgagcccc atggcttacg cttcgccgac tcgcgcgagc ggcctctgct
     3961 gatgagccct ggataccgca gaactcacct cgtgagcggg gcctcgtagc aggcccacgc
     4021 gacgtgcgac tcgtgcaagg tcaccttcat cgactcgagt tgctcactgc ccggtccgag
     4081 ttcgcctttc gcacagcggt cggcgaagcg ctcgaacagc gtaaacgcca ggaactcggt
     4141 ggtggtgttt acgccggaga aggcgggcat ttcatccaag ttctggaagt tcagctcgcc
     4201 gagcaaagag ttcaagacat cggtcgctct accaatgtcg acgaccacgc cgtccgccga
     4261 caagttcttg cgacggaact cgacgtcgac cacgtaggtc gccccgtgca agcgctgcgc
     4321 agggccgaat agctccccgc gaaagctgtg agcaatcatc atgtgtccgc gaactgaaac
     4381 ggtaaacatc gcttagcctt ctgttggcgc cttctactga cggctatcta acgcaaacgg
     4441 tgctgggcat gcacgctggt cggcaacata ctggccggca gacgagctag agaaagaggg
     4501 tcgcaccgac gaactcagaa gtagcgtaca cgctggcaaa gcgcccgcgc gcccggggcc
     4561 gtgatctcgg ccatcaccgc ggggagttcg tcgaatgtgc actcgccgtc gatcagcacg
     4621 tcgagcgcac tatcagcgca tagggcgagc gccagctcca gtcgcttccg catgtcgaac
     4681 cgagctctgg cgttcgggct caagcgaccc acttgcgagc tcctgagcgt caaacgcgcc
     4741 gaatgaaatc tctggccgag cggcgcggcc acttccgcgt cgccgtacca actcagctcg
     4801 acaatcgtcg tctccgcgcc tgcgatctcc aatgccgccg ccaaacccgc aggtcgcccg
     4861 ctggcatgaa acacgagatc ccgatcccgc tcggcccgtt cgggcgtcga gaaacgaacg
     4921 ccaaaagcgt cggcgacgcg cctgcgctcc ggcagaacgt cgatcaactc gacctcggtc
     4981 tggaggttac gggacaatag gaaggccagc agcgaaccca acaccccggc tccgaccacg
     5041 ctgactcggt caccgaggcg cggca
//
LOCUS       DASTKU010000042         6053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_676077, whole genome shotgun sequence.
ACCESSION   DASTKU010000042 DASTKU010000000
VERSION     DASTKU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676077"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="VFQ61_01395"
     CDS             <1..1554
                     /locus_tag="VFQ61_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET9953123.1"
                     /translation="PGREDAWWRSSALPSMLLFVASVTLDPKSIRAFENDEAFEAWLS
                     RYHDQKTELYLRIFKKDSGKPTVTYAEALDVALCWGWIDSVKHSYDDESFLQRFTPRK
                     FHSRWSEINCQNVERLTREGRMTRHGQRHVDSAKADGRWEAAYASPSNMQMPEDLMAA
                     IEANPKAFATFESLNKVNRYALAYRTHHVTDPDKRIEKIQKFVEMLERGESPHPNSRP
                     EDDAKLNGAKGKLLEEMLGDEDTGASAAASGGPASKRQGGPASKRPAAESSAADKELA
                     PAPSKQAARPRAATSSIKKNAVKKATAQSAAKSAKPSRGVSKLPVKPALTKASAKSAT
                     NKASSRASVAKPASAAKAASKAPAKSASKSAFGKPPLKKAPAPKASALASRAMGSRAV
                     AASSGAKKGAVGKTTAAKTTAAIPTAKKPAANGATGKPAGKSAVAARVSKAVAKQASP
                     QRGFALSGSGLSAAALSGSASSGSKKSPSAKRAVASKPIAVSKQIPVSKQPAANRQFP
                     AKKLRTGRG"
     gene            1547..1942
                     /locus_tag="VFQ61_01400"
     CDS             1547..1942
                     /locus_tag="VFQ61_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953124.1"
                     /translation="MVEIGEKLQRADPLRALVHQVIDELLTKGGPLIPLEVLIQLELV
                     GADEVAAWRNGELPYLERGMTSGLSRVARVLRLIEERALELGLTATRGKYVRRGPGRK
                     QSLRFSKRGDAESERLYSRHFVRPAGAER"
     gene            2399..2551
                     /locus_tag="VFQ61_01405"
     CDS             2399..2551
                     /locus_tag="VFQ61_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953125.1"
                     /translation="MKVPEFRGVSQIRRVQQSADQQSADQQSADQQSADQQSADQQSA
                     AVGGRV"
     gene            2707..4194
                     /locus_tag="VFQ61_01410"
     CDS             2707..4194
                     /locus_tag="VFQ61_01410"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9953126.1"
                     /translation="MSLRAVEKQPEQQVGRYAIFDRLASGGMASVHIARLLGREGFSR
                     VVAAKRMHRHFLEDSAFRRMFLEEARLAARVRHPNVVPIVDVLSQGDEIILVMEYVHG
                     ESLQALTRLASGAPFPIPIACAIMTGTLHGLHAAHEARNERGEPLGIVHRDVSPHNIL
                     VGADGVVRVADFGVAKAVHAREDSAPGVVKGKVSFLAPEVVRGAPATRRSDIFSAATV
                     FWELLAGRRLFAGATEQERLLAIVNGNYPALSAFNARVPEALERLVAKGLMLEATERY
                     ETALEFAIEVEREVQLASQRAVGDWVERLAADELERRANLIHEVETSIIGPISMVRNR
                     LASDALDEIEEIEELSEAVAVLGSESKDGGRGSKAAPSAPRTTTSARWLPVLLGLTGL
                     VLLTLALLIGLRTNGEAQPSAKTGPSGLSPPASLPSVDTGKAPNHPPTVASGSTPLTA
                     SAPAESAPSATVRAAPSSSNPRPAPPPSAAPHTKIYGKPFLPSEL"
     gene            4163..5206
                     /locus_tag="VFQ61_01415"
     CDS             4163..5206
                     /locus_tag="VFQ61_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953127.1"
                     /translation="MANPSYRASFSPAAACAWLALLGASSWPEVCRAQGPIAQSATGN
                     VATNVSSEAGVLYAEAIKSFKAGEAAKALPMFERVVGLTQSPNAELYVGYCLVELNRY
                     PEAYRAFSAAMQHATALADAQGVSKYDATREAAENQLLALNLRVAKLILSFVDMPEDL
                     VVRVDDVAIDVAELGSPIVLQPGLHRVEALSNGHQAILREVQIETGGSKTLTLALGTR
                     KAEPAPALLAPTTVVAKPERGVRLETFGFVAAGVGAAGLAVFAVTGLQAKSLHDRLER
                     ECPQGCSDAVHLDAAGRGKSLQTVANTSLGVGAVGLLSSAVLLYLGARQTHEVPRIAL
                     APGDARLLYEGRF"
     gene            5209..6018
                     /locus_tag="VFQ61_01420"
     CDS             5209..6018
                     /locus_tag="VFQ61_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953128.1"
                     /translation="MTAERAAVCGSAFLLLVACSYDFERFVSEDSRTNAGGMPTDAGG
                     MPSGGRPSTGGRSETVSGAGEGSENPDSVQGGNAVARGGSASTGGSVPNGSFAEGGTS
                     AMGSGGRSDGSTTQGAVNSGGAATGGTVTAGTDASGGTASSAGQTSTSGGNEPECAGK
                     RVLDVCWYLGKFDESCTQVCESHGGVADAALVVIGVSEQGGSAAECRQVLDAFEQGGA
                     LSQGTRTDGLGLGCFLRVERNDRTTRWWLSAPAYDVDAHHTTARRACGCAR"
BASE COUNT         1155 a         1820 c         2053 g         1025 t
ORIGIN      
        1 ccggggcgtg aagatgcgtg gtggcgttcg agcgcgctgc cgtctatgct cctcttcgtg
       61 gccagcgtca ctcttgatcc gaagtccatc cgcgcattcg aaaacgacga ggcgttcgag
      121 gcatggctgt ctcgctatca cgaccagaag accgagcttt acctgcgcat cttcaagaaa
      181 gacagcggta aacccacggt cacctatgcg gaggcgttag acgtcgccct gtgctggggc
      241 tggatcgata gtgtcaaaca cagctacgac gatgaatcgt tcctgcagcg cttcacgccg
      301 cgcaagttcc atagccgttg gtccgagatc aactgtcaga acgtggagcg tttgactcgc
      361 gaggggcgca tgacccgaca tgggcagcgc cacgtagatt ctgcgaaggc ggacgggcgc
      421 tgggaggccg cctatgcctc tcccagcaac atgcagatgc ccgaagatct catggctgcg
      481 atcgaggcca acccgaaggc gtttgccacc ttcgagtcgc tgaacaaggt gaaccgttac
      541 gccctggcgt atcgaacgca ccacgtgacc gatccagaca agcgcatcga gaagatccag
      601 aagttcgtcg agatgctcga gcgtggggag agcccgcacc cgaactcacg ccccgaggac
      661 gacgccaagc tcaacggcgc gaagggcaag ctgctggaag agatgttggg tgacgaggac
      721 accggtgcca gcgccgccgc gagcggaggc cccgcgagca agaggcaggg cggtccagcg
      781 agcaagcggc cggcagccga aagcagcgct gccgacaaag agctcgcacc tgcaccgtcc
      841 aagcaggcgg ctcgccctcg cgcggccacg tcatccatca agaagaacgc cgtaaagaag
      901 gcgacggcgc agtcagcggc caagtccgcc aaaccgagcc gaggcgtcag caagctgcca
      961 gtcaagccgg cgctcaccaa agcttcggcg aagtctgcga cgaacaaagc ctcctcacga
     1021 gcatcggtcg ccaagcccgc atccgcggcc aaagcggcct cgaaggcacc cgccaagagc
     1081 gcctccaaat ccgccttcgg caagccaccg ctcaagaagg ctcccgcacc caaggcgtct
     1141 gccctcgcga gcagagccat ggggagcaga gccgttgcgg ccagctccgg ggcgaagaag
     1201 ggtgcagttg gcaaaaccac ggccgccaaa accacggccg ccattcccac agcgaagaaa
     1261 cccgctgcaa acggagccac agggaaaccc gcgggcaaga gcgcggtcgc ggcgcgcgtc
     1321 tccaaagccg tcgcgaagca ggcttcgccc cagcgcgggt tcgctctttc tggctctggt
     1381 ttgtccgccg ccgcgttgtc cggctccgcc tcgtccggct ccaagaagtc gccctccgcc
     1441 aaacgagcgg tcgccagtaa gccaatcgcc gtgagtaagc agatccccgt gagcaagcag
     1501 ccggccgcca atcggcagtt tccggcaaag aagctcagaa ccggtcgtgg ttgaaatcgg
     1561 cgagaagctg caacgagcgg atcctctgcg ggcgctcgtc catcaggtca tcgacgagct
     1621 gctgacgaaa ggtggcccac tgatcccgct cgaggttttg atccaactcg agctcgtcgg
     1681 cgccgatgag gtggccgctt ggcgcaacgg ggaactgccc tacctggagc gaggcatgac
     1741 gagcgggcta tcgcgcgtcg cgcgcgtgct gcgcttgatc gaggagcgcg cgctggagct
     1801 gggtctgacc gctacgcgag gcaagtacgt gcggcggggc ccgggccgca agcagagctt
     1861 gcgcttctcg aaacgaggtg acgctgagtc ggagcgactc tacagccgtc acttcgtgcg
     1921 tccagctggg gctgagcgct agcgcacccc acggttcgaa acgcgctgca agcgccaatc
     1981 cgctcgcggc gacggcggag gtgcgggcga gttcgggagc acgcttgcga ttgcgtgtga
     2041 ttgtattgcc aggggcgcag gcaatgcgag cttgcgttac agggtggccc aagcgcggtg
     2101 catcggtgca ctcgaggaga acgcggctgc gctcgaggtt catgtctcgg ggttgcgcgc
     2161 ccgtttactc gactgcccac cacaaaggca ctcactacac tcgccagctc gaagcgttac
     2221 aaatcgagcc ggatcacaaa tcctgcgggt tgcgggcgaa acgctattac aagaagtgcg
     2281 ataaacggcc gttgcactcg ggagttgcgc cgctcgcgcc gcctgaacca aatttcgcga
     2341 tgttacgagg attgagcttg cggcgttcgg tcgcgtttca ccgagccaac ggagtttcat
     2401 gaaggtccct gagtttcgcg gagtttcgca aattcgccga gttcaacaga gcgcggatca
     2461 acagagcgcg gatcaacaga gcgcggatca acagagcgcg gatcaacaga gcgcggatca
     2521 acagagcgcg gcggtagggg gacgggtgtg agttgtgcgc gcgcggctcg cggtgtcaca
     2581 ccctaggtct gatatcagcc tgctgatatc tgcacgagta gcgaccgagt caggttgacg
     2641 ctgtcagaga gtaggctcta aactcaaaag agtttcgata ttcgcgctgg gagtacgcga
     2701 accgccatga gcttacgagc tgtcgagaag caacccgaac agcaagtggg tcgatacgcg
     2761 atcttcgatc gcctcgcgtc gggtggcatg gcgagcgtac acatcgcgcg gctgctggga
     2821 cgcgaaggct tttcgcgcgt ggtcgccgcc aaacgaatgc accgtcactt cttggaagac
     2881 tcggcgtttc gtcgcatgtt cctggaagag gcacggctcg cggcccgcgt gcggcacccc
     2941 aacgtggtgc cgatcgtgga tgtgctgtca cagggggacg agatcatcct ggtgatggag
     3001 tacgtccatg gggagtcgct acaggcgctg acacggctgg cgtcgggggc accgtttccc
     3061 atccccatcg cgtgcgcgat catgacgggc acgctgcacg ggctccacgc ggctcacgag
     3121 gcacgcaacg agcgaggtga gccgctcggc atcgtgcatc gcgatgtctc cccccacaat
     3181 attctggtcg gcgcagacgg agtcgtgcgc gtcgcggact ttggcgtggc caaggcggtg
     3241 cacgcgcgcg aggatagtgc gccgggcgtg gtgaaaggaa aggtgtcttt tctcgcgccc
     3301 gaggtggtgc gcggtgcacc ggcgacacgg cgttcggata tcttctctgc ggccacggtg
     3361 ttttgggagt tactggcggg gcgtcgtctg ttcgcggggg ctacggagca ggagcggtta
     3421 ctcgcgatcg tgaatgggaa ttacccggcg ctgagcgcgt tcaacgctcg agttcccgag
     3481 gcgctcgagc gtctggtcgc caaaggtttg atgctggagg cgaccgagcg ctacgaaacg
     3541 gctctggagt tcgcaatcga ggtcgagcga gaggtgcagc tcgcgtcgca acgcgcggtg
     3601 ggggattggg tggagcggct ggccgccgat gagctcgagc gtcgcgccaa tctgattcac
     3661 gaggtggaga cctcgattat cggtccgatc tcgatggtcc gtaatcgtct cgcgagtgac
     3721 gcgctcgacg agatagagga gattgaggag ctctctgaag ccgtcgccgt cttgggtagc
     3781 gagtcaaagg acgggggccg cggctccaag gccgccccga gtgcaccccg aacgacgacg
     3841 tcagcgcgct ggctgcccgt gttactcggc ttgaccgggc tcgtgttgct gacattggcg
     3901 ctgttgatag ggctgcgaac aaatggcgaa gctcagccga gcgccaagac aggcccctca
     3961 gggctttcgc cgcctgccag tttgccgtct gtggatacag gtaaggctcc gaatcatccg
     4021 cctacggtgg cgagcggtag cacgccgctc accgcgtctg ctccggccga gagcgcgccc
     4081 agtgcgaccg ttcgcgcggc accgagctcc tccaacccac gtccggcgcc gccgcctagt
     4141 gcggcgcctc acaccaagat ctatggcaaa cccttcctac cgagcgagct ttagccctgc
     4201 tgcggcgtgt gcctggctcg cgctgctggg cgcctcgagc tggccagaag tatgtcgagc
     4261 gcagggcccc atagcgcaga gcgccaccgg gaacgtggct acgaacgtga gctccgaggc
     4321 tggtgtgcta tacgcggaag cgatcaagtc cttcaaggcc ggcgaggccg cgaaggcgct
     4381 gccaatgttc gagcgggtgg tgggcttgac gcagagcccg aacgcggagc tgtacgtggg
     4441 gtactgcttg gtcgagctca atcgttaccc cgaagcctat cgggccttct ctgcggcgat
     4501 gcagcacgcg accgcactcg ccgatgcgca gggagtgagc aagtacgacg caacccggga
     4561 ggcggcggaa aatcagctct tggcgctgaa tcttcgggtc gccaagctaa tcttgtcctt
     4621 cgtcgacatg cccgaggact tggtggtcag ggtagacgac gtagccatcg atgtggcgga
     4681 gttgggatca cccatcgttt tgcagcccgg tctccatcga gtcgaagcgc tctcgaatgg
     4741 tcaccaagcc attctccgtg aggttcaaat cgaaaccgga ggtagcaaga ctttgacgct
     4801 ggctttgggg acgaggaagg cggagcccgc tcccgcgcta cttgctccca ctacggtcgt
     4861 ggccaaaccc gaacgcgggg ttcggttgga gacctttggg ttcgtcgccg caggcgtagg
     4921 tgcggctggg ctcgccgtat ttgcggtgac cggtctgcaa gcaaagagtt tgcacgatcg
     4981 tttggagaga gagtgccccc aaggttgctc ggacgcggta catctggatg ccgccgggcg
     5041 cggcaagtcg ctgcaaaccg tggcgaatac cagcttgggc gtaggcgcgg tgggattgtt
     5101 gagcagcgcc gtgttgctgt atctcggcgc ccgccaaact catgaggttc ctcgcatcgc
     5161 cttggctccg ggagacgcgc ggttgctcta tgaagggcgg ttttagcagt gactgcggag
     5221 agagcagcgg tctgcggctc tgcgttcttg ctgctcgtcg cgtgttcgta tgacttcgaa
     5281 cgcttcgttt ccgaagactc gagaacgaac gcaggcggaa tgcccacgga cgcaggcgga
     5341 atgcccagcg gcggacgtcc gagcacaggc gggcggtccg aaaccgtttc gggtgcgggc
     5401 gaggggagcg aaaacccgga ctccgtgcaa ggtggcaacg cggtagcgag gggcggttcc
     5461 gcgtcgacgg gcggctctgt acccaacggc agcttcgcag agggtggcac cagcgctatg
     5521 ggtagtgggg gacggagcga cggctccacc acacaggggg ccgtaaactc tggcggtgcc
     5581 gcgaccggag gtaccgtcac cgcgggtacg gatgcaagcg gtggcactgc gtcgtccgca
     5641 ggtcaaacga gtacttccgg gggaaacgag cccgagtgcg caggcaagcg cgtgctagac
     5701 gtttgctggt acctcggcaa attcgacgag agctgcactc aggtgtgtga gtcgcacggg
     5761 ggtgtcgccg atgctgcatt ggtggtgatc ggtgtatctg aacaaggggg gagcgctgct
     5821 gaatgccgac aagtcctcga tgctttcgag caggggggcg cgctcagtca gggaacgcgc
     5881 acggacgggc tgggccttgg ctgcttcctg cgcgtcgagc gcaacgatcg tacgacgcgc
     5941 tggtggttgt ccgcgccagc ctatgacgtc gatgcacacc acacgaccgc gcgaagagcg
     6001 tgcggctgcg cgcgctgaat caacggcata ccgacggcgc ccaaatccgt caa
//
LOCUS       DASTKU010000043         8298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_230217, whole genome shotgun sequence.
ACCESSION   DASTKU010000043 DASTKU010000000
VERSION     DASTKU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230217"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFQ61_01425"
     CDS             complement(<1..597)
                     /locus_tag="VFQ61_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953129.1"
                     /translation="MPDNRALRTLFDTYWSPSGWRPEGERLQNPGAIEHAKRCGVMFE
                     PTIIDHDEVITRLISARDRLNPRLVGDAFLASLTTRRLDLRSALGSYSVFRHMPVHRA
                     NSAVGACSVCGLFGSSGQSEDLNVLNFERFKWGGVRHDQPLYATLDLELLLKERPPHP
                     TNEDIRLLKLILEALRDAPPSTTSAQVQRFLPKALKSNK"
     gene            890..1117
                     /locus_tag="VFQ61_01430"
     CDS             890..1117
                     /locus_tag="VFQ61_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953130.1"
                     /translation="MPWQPPVWNNALPLRRQLPEGDWRVAIEEIVAKHGLAPCELEPY
                     ARGETIVWRAGHHVVKMTAPQFAYQIAAVGT"
     gene            complement(1274..1807)
                     /locus_tag="VFQ61_01435"
     CDS             complement(1274..1807)
                     /locus_tag="VFQ61_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953131.1"
                     /translation="MKPSFGAPWALTLRPGGRGAFSLRVTRLSRDVWAAMMKRMSELQ
                     GLTVNLSAHHQRRALADVSVQVATAERHIDPATANLLSMLWRALIARSQVVETEVVTV
                     DGTGYSIMVGGRTGSTQSPVYGSILERTTYAAERLAHFVESPEADDESDLVFVREELK
                     EALARTRAQEPCVRMVK"
     gene            2357..3631
                     /locus_tag="VFQ61_01440"
     CDS             2357..3631
                     /locus_tag="VFQ61_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HET9953132.1"
                     /translation="MPTAVWALRWTDVDPATRAFDEGAVRAIVKPLLSAPLTTSEARR
                     AAEDRIDRALLAELGHWVAGWRWAASEPGCGGPVRAYCCADHSLKRSPDAMREVVVGA
                     VRAWRGRIVEMSALFMKLREEAAGLDIDEAASRAATSLLPVVLEWTGAEDAWYSTYER
                     VLAWYLESLLGESDRTLTLVVQAIGGRFQSWLEPDKAQAEAVCRDIGASFAQEAVAEP
                     THVDSLREWLGEREKIRWGRERICHPEPVSRDGHQRFIDTLDSARSPERARRMSQALE
                     AARRAAHHDIPLTFDLIRQWQSLVLGTEVEFRDGVAFSHGGAERYGLDAHTRRRFDQC
                     LAEATESSLPSVVRAARVYLDVCYFHPFPDGNARAARLALDYVLTRDGLALHAAEPIF
                     LVARRVGDKHGVYGFIYVVDYLAGAANLLGRK"
     gene            complement(3819..4085)
                     /locus_tag="VFQ61_01445"
     CDS             complement(3819..4085)
                     /locus_tag="VFQ61_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953133.1"
                     /translation="MGYEYQLDFEHCEPALADQILRAIPGFEKCTAGLYSFRRNATGQ
                     MPDAYAAVEPTGVYVCDNGGAPQIVNDIRAAFVAAGLHAELREL"
     gene            complement(4276..5247)
                     /locus_tag="VFQ61_01450"
     CDS             complement(4276..5247)
                     /locus_tag="VFQ61_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953134.1"
                     /translation="MPSSDHPSGADPSADAVGQEQAAIEAVERLRPFYQALPRHELLR
                     INIDIPSAIVTVLGALPQIRKLRPSVCQQTPEHDMRLFDSIEDAALALNELHAQYLLA
                     TRPPDGLQQLITEATELRDRLLADVSALTTRGFIDRNDLVELKRTSGHRNLAFDLNLL
                     QLLLRRSWAQIQGKSAVTVSELDRASKLQQRILRVIGERALTTSTITSARDLRQRAFT
                     YFVHAYDQTRRAITYLLWDSPNPSAIPPSLYSGRGNRKRKTPTLTVAPHDSSPSHTIA
                     PGSRPPEVETRTEGAVQATGTASASMRERSGNSGGGLGGAGEDPFLN"
     gene            5881..6543
                     /locus_tag="VFQ61_01455"
     CDS             5881..6543
                     /locus_tag="VFQ61_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9953135.1"
                     /translation="MASLDEVKRALAGERAALEALIRELSPVVHARVATALWRRGHGS
                     RQELEDLTQEVLMLLFTGKERGLAGWEPERGLSLAAFAGLVAQRLVCSILRSRGRSPF
                     SQEPADPATLDVALSATHVQPQPEGPVAARELLGLLFERLQVELSPKGLEMFYRLYVW
                     QQEPELIAEETGAKLEAIYQWRTRLKKEVARLELSLMGPGRPLALVKDGPHNRSKSDV
                     TS"
     gene            6530..7282
                     /locus_tag="VFQ61_01460"
     CDS             6530..7282
                     /locus_tag="VFQ61_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953136.1"
                     /translation="MSRPDLTIDDLAQFAREDAAVQAAMERRAKGAPTPDDDLLLEEV
                     PDFLLPSSSPLETAQLVASIQERLGAERPSEFTPSTTPGRTASKPRRMTWLLSASAGV
                     VALAAAVLLWVGAPPPEKLAHYTVQVEGSQRGERAASHEQPVVLRLRSGSELRFALRP
                     ETDVDGEIRARLFARRSDGSGTVELLIRQEQSAAGALRVESTVPSTLPESGELVLVVG
                     RAETLRGLSASATLDVTERSGQAFRWRFEHSL"
     gene            7295..>8298
                     /locus_tag="VFQ61_01465"
     CDS             7295..>8298
                     /locus_tag="VFQ61_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953137.1"
                     /translation="MFGLRAPAFLRAPPRVRLWIALFAFTAFAAARDARPPRADHVLM
                     FEYAGCARVRPGPRCELGPERELTLWVPGKRSLAFAAPGAPPVSVHESRFIEGGQRTR
                     VRVPGGAKVLQIRAGESQAELLVAESSEPAPLSELRRLWQAGKWEEVKGRLTEVESRL
                     STVERSRLQAFRGRLALRAGEHERAAAELEAAAKSARSAGLWLEASNDLLAAAYCRAA
                     RLLQFERARALLDGLPRELAQVPETKARIPYYLGVIARTRGDVQGALLQFRRASVFAH
                     RLGLASEEGMARQELAVVLNLLGRRAEALAEQRELAVHAEREPNCLRPIRWENLAWML
                     L"
BASE COUNT         1493 a         2418 c         2775 g         1612 t
ORIGIN      
        1 tttgttcgat ttcaatgctt tgggcaggaa ccgctgcacc tgggcagacg tcgtggaagg
       61 aggcgcgtct cgcagggctt ccagtattag cttcaagagc ctaatgtctt cgttggtcgg
      121 atgcggaggt cgctctttca ggaggagttc gagatcgagc gtcgcgtaca atggctgatc
      181 gtgacggact ccaccccact tgaaacgttc gaagttcaaa acgttcagat cttctgattg
      241 accagatgat ccgaaaaggc cgcacacgga gcaagcccct accgcagagt tcgctcgatg
      301 taccggcatg tggcggaaaa cactgtagct ccccagcgca gaacgcaggt cgagccggcg
      361 agtcgtcaag ctcgctagaa atgcatcgcc caccaggcgg ggattgagcc tgtcacgggc
      421 gctgatcagg cgtgtgatga cctcatcgtg atcgatgatc gtcggttcga acatcacgcc
      481 gcacctcttc gcgtgctcaa tggctcctgg gttctgcagg cgctcgccct ccggtcgcca
      541 accagagggg gaccaatagg tgtcgaacag cgttcgaaga gcacggttgt ccggcatgcg
      601 cgtccagctg cgcagcgtac cgttccaaag ctagagccgc atcaggaaag cgtgacgcca
      661 cgcgcaagac atggccgcct tccgctcgcg gccatgcggg ccggctaccg caccggctcg
      721 ctgccgaagc ggccgccggt gccagtacgt tgcccattgc tttccacgta gaggtcatcg
      781 tcgtcgtctg gatccattcg gaccaagacg cgcttgatta tgctggtatg agctctgact
      841 atcgtgagat taagcagaat ctactgcgat gctgcgatat aggactgata tgccttggca
      901 gccacccgta tggaacaacg ctctcccgct tcgaaggcag ctgccggagg gtgattggcg
      961 agtggcgatc gaggagatcg tggccaagca tggcctcgcg ccgtgtgaac tcgaaccgta
     1021 tgcacgaggc gagacgattg tgtggcgcgc tgggcaccac gtggtgaaga tgaccgcacc
     1081 acaattcgcg tatcagatcg cggccgtagg aacctgaaga catcctttct gtttgccatc
     1141 agtgctcccg cggctcgtct gttcgctcgc gattacgatt gctgaactga aggagtcaaa
     1201 cggtcggaag ggcttccttc ttcccgtccg cgatctggtt tcgcgcgcaa gaacgggcgg
     1261 tgcaggcgga ggattatttg accattcgaa cgcacggctc ctgggcgcgt gttcgtgcga
     1321 gcgcctcctt cagctcctcg cgcacgaata cgaggtctga ctcgtcgtcc gcctctgggg
     1381 attcgacgaa gtgcgcgagc ctttccgccg cataggtcgt tcgctccaag atgctcccgt
     1441 agacggggga ctgcgtcgag ccggttcggc ctcccaccat aatggagtat cccgtaccat
     1501 ccactgtgac gacctcagtt tcgaccacct ggctacgcgc aatcaatgct cgccaaagca
     1561 tgctgagcag attcgcggtt gctggatcta tgtgacgttc ggccgttgcc acttgtacgg
     1621 agacatccgc tagagctcga cgttgatggt gcgccgaaag gttcacggtg agcccctgca
     1681 gttcactcat gcgcttcatc atggccgccc acacgtcgcg gctcagccgt gtgacgcgca
     1741 aggagaaggc tccgcgaccg cctgggcgga gcgtgagcgc ccagggtgca ccaaaggacg
     1801 gtttcacgac tatcgccaca gcggatctcg ggatttcgag agcgctaagt acgaatctgt
     1861 cgtatgggtc aaacccttct tccgcgcgtt caggttggag gcaggccccc gaatcggtac
     1921 ggacaagagt ctcgactgta gggcccgcgt gatgctcgtc gttccacgat gcactgaatg
     1981 gctctgccgc cactgggagt tcatctgtcc gcgcatctag ccgcgatcga tgcgtgggaa
     2041 cgcgatcggg tgcggagttc gcctccacgg gtggaactgg tggccgcgca tgcgtgcacg
     2101 ccagggatgc aaagccgacc aggagcttcc tacgagctga aaacacagca cgggagacaa
     2161 cggagattga cggaggttcc ccgtgtactc cgtttgccag aatctcgtat gagctgcgca
     2221 tccagaagta ctctctatgg ttggagcgag ctttacgaaa gaggcgacac ttttgcactc
     2281 agccaccgag ctcatccgcc aattccatcg aatggtgccc gtgccctgat ttaaggcttc
     2341 tgctaagagc tttcgtatgc cgactgccgt ttgggcactg cgctggacgg acgtggatcc
     2401 cgcaacgcgc gctttcgatg aaggcgctgt tcgagccatc gtgaagccgc tgctctcggc
     2461 gcctctgacg acgtccgagg cgcggcgcgc cgctgaagac cggatcgatc gggccctgct
     2521 ggccgagttg ggtcactggg tggcgggctg gcgttgggcc gcgtcggagc ccggatgcgg
     2581 cggccccgtg cgagcgtatt gctgcgcaga ccacagcctg aagcgtagcc ctgatgcgat
     2641 gcgtgaagtc gtcgtgggtg cggttcgagc atggcggggc cgcatcgttg aaatgtccgc
     2701 cttgtttatg aaactgcgcg aagaagctgc gggattggac atcgatgagg cggctagccg
     2761 agctgccacg agcctgctgc cggtggtact cgagtggacg ggggcggagg atgcttggta
     2821 ctcgacgtat gagcgtgttc tggcctggta cctcgagagc ctgctgggtg agtccgatcg
     2881 aacgctgacc ttggttgtgc aagcgatcgg cgggcgcttc cagagctggc tcgagccaga
     2941 caaggcgcag gccgaagcag tgtgtcgcga catcggagcg tctttcgcgc aagaagccgt
     3001 cgcggagccg acgcacgtcg atagcttgcg tgaatggttg ggagagcggg agaagattcg
     3061 ctggggcaga gagcgaatat gccaccccga gccagtgtcc agagacggac accaacgctt
     3121 cattgacacc ctggatagtg cgcgcagtcc cgaacgagcg cggcgcatga gccaagcgct
     3181 cgaggccgcg cgccgagccg cgcaccacga tattcccttg accttcgact tgatccggca
     3241 atggcagtcg ttggtgctgg gcacagaggt cgagtttagg gatggcgtgg cgttctccca
     3301 tggcggcgcg gaacgatatg gtctggatgc gcatactcgg cgtcggttcg atcaatgcct
     3361 ggccgaggcc acggaatcgt cccttccatc cgtcgtacgc gcagcccgag tctacctcga
     3421 cgtttgctac ttccatccgt ttccggatgg gaatgctcgg gcggcgaggc tcgcgctcga
     3481 ctacgtgctc acgcgcgatg gactcgccct gcacgcagcc gagcccatat ttctcgtcgc
     3541 acgccgggtc ggagacaaac acggcgtcta cggattcata tatgtggtgg actatctcgc
     3601 tggggctgcc aacttgctcg gacgcaagtg attcggtgat gctgcgtagc tccgaccagg
     3661 cgtgaagaac gcgcattcgg gtcgagccat gcgaggctac gcttaagttc ccctgatcga
     3721 gcccgctgca tgacggtggg ctacgtgctt gagccatggg agaaccctcg gtccgcttag
     3781 acatgacctc gagaacgctg cagtcggaac ttcattccct agagctcgcg gagctccgcg
     3841 tggagccccg ccgccacgaa agcggctcga atgtcattca cgatctgggg cgccccgccg
     3901 ttgtcgcaca cgtaaactcc agtcggttcg accgcggcgt aggcgtctgg catctgaccg
     3961 gtcgcgttac gccggaatga gtagaggccg gccgtgcatt tctcgaaccc cgggatcgcg
     4021 cgcaagattt gatcggccag cgctggctca cagtgttcga agtccagctg atattcgtag
     4081 cccatggcat ctcggtcgag caggttggct tagtgatgcg agtacccccc gcgcaatgca
     4141 ctctcctcta tcacgtttag gattcagcct cgatccggta cgcgaaaccc ggcgcagctt
     4201 gcgtgccaga agatggcccg aagccaacac gcccactgcg aaccaagtgg acacgtagtg
     4261 actgcccctc cagcctcaat tcaaaaacgg gtcctcccct gctccgccca agcctccgcc
     4321 cgaatttcct gaacgctctc gcatcgaagc cgaagccgta cccgtcgctt ggaccgcgcc
     4381 ttccgtacgt gtttcaacct ctggaggcct cgaaccgggc gcgatcgtgt gcgaaggact
     4441 cgaatcgtgg ggtgcgaccg tcagggtcgg ggttttgcgt ttgcgattgc cgcggccgga
     4501 gtagagggac ggggggatcg cggacgggtt ggggctgtcc cagagtaggt aggtgatggc
     4561 gcgccgggtt tgatcgtacg catgtacgaa gtaggtaaag gcgcgctggc gaaggtcgcg
     4621 agctgaggtg attgtggagg tcgtgagcgc ccgctctccg atgacacgga ggatgcgctg
     4681 ttggagtttg ctcgcgcggt cgagttcaga aacggtgacc gcagatttgc cctgaatttg
     4741 cgcccagctt cgacgcaaga ggagctggag gaggttcagg tcgaaggcga ggttgcggtg
     4801 gccagaggtt cgcttgagtt caacgagatc gttgcgatcg atgaagccgc gtgtagtgag
     4861 cgcactaaca tctgcgagga gtcggtcgcg gagttcggtg gcttcggtga tgagctgttg
     4921 gaggccgtcc ggcggacgag tggcgagtag atattgagcg tgtagttcat tgagggccag
     4981 cgcagcgtct tcgatgctat cgaatagtct catgtcgtgt tcgggggtct gctggcacac
     5041 gctggggcgc aatttgcgga tttgaggcaa agcgccgagc acggtgacaa tggcggaggg
     5101 aatatcgatg ttgatccgaa gaagctcgtg gcgaggtagg gcctgataga agggcctgag
     5161 acgctctacg gcttcgatgg cagcttgctc ttgcccgaca gcgtccgcgc tggggtcggc
     5221 ccccgatgga tgatctgaac tcggcatgtt taccccaata cgaccaggca tacgcccgaa
     5281 actggtttcc ccttccccag tccggagcag tcgccacaca cggccgcgat ctccgattcg
     5341 ctcggacctc tcaataggca tcaccccgca gtaacgaggg gtgacacccg gcgacgcaac
     5401 cgcgctggcg agcaagggca acttgcaggg gttaaggctc gctgttccgg cgaggacggt
     5461 ccgattacag ccgtgagctc caagtgctgg gcgcgcccat gattcgagcg gctggtgata
     5521 cctgatgccg tctacacggt cttgtacttc ggactagtga gagtctggcc ccgtcggacg
     5581 ggatattttc agccacttcg gagcgcaggc gacctgcggt cgggagtttc cgcttaccgg
     5641 ctgttcgtgg acgctcgcgt tgcacctgat cgaactaccc agtgcgccac gcaccgtgcc
     5701 caactgcaat ggtgctgctc tgttacgctc gttcgcgcga gctccgctcg gcgaggcaaa
     5761 ggcggttgct tgttctgtag gggcaggctg cctcgagggc cagcgcagca ttggagtgca
     5821 taaggcaggt atgaattgcc tataggcgcg gggccggatg gagagtagtg acgaagagga
     5881 atggcttcgc tcgacgaggt gaagcgggcg ctcgccggtg agcgcgctgc actggaagcc
     5941 ctgattcgag agctgagtcc cgtggtgcac gcgcgagtgg ccacggcgct ttggcgccga
     6001 gggcatggat cccggcagga gctcgaggat ctaacccagg aggtgctgat gctgctgttc
     6061 accggcaagg aacgaggcct cgccggttgg gagccggagc gcgggctgtc tttggccgcg
     6121 tttgctggtt tggtggcgca gcgtttggtg tgttcgattt tgcggagccg cggccgaagc
     6181 cctttcagtc aggagccggc agatccggcg acgctcgatg tggcgctgag cgcgacccac
     6241 gtgcaaccgc aacccgaggg tcccgtcgca gcgcgcgagc tcttgggctt gctcttcgaa
     6301 cgcttgcagg tggagctgtc gccaaagggc ctcgagatgt tttaccgact gtacgtgtgg
     6361 cagcaggagc cggagttgat cgcagaggag acgggcgcga agctcgaagc catttaccaa
     6421 tggcgcactc gcttgaagaa agaggtcgcg aggttggaac tgagcttgat gggccccgga
     6481 cgccccctcg cgttggtcaa ggatggtccc cacaaccgga gtaaatccga tgtcacgtcc
     6541 tgatctgacc atcgatgatt tggcgcaatt cgcgcgcgaa gacgcggccg tgcaggcagc
     6601 aatggagcga cgagccaagg gcgcacccac gcctgacgac gatctgttgc tcgaagaggt
     6661 cccggatttt cttctgccct cgtcttcacc cctcgaaacc gctcagctcg tcgcctcgat
     6721 tcaagaacgg ctcggcgcag agcggccaag tgaattcact ccatcgacca cgccggggcg
     6781 aaccgcttcg aagccacgtc gcatgacatg gctgctgagc gcgtccgcgg gcgtcgtcgc
     6841 gttggcggca gcggtgctgc tttgggttgg cgctccgccc cctgagaagc tcgcacacta
     6901 tacggtgcag gtggaggggt ctcaacgcgg cgagcgggct gccagccatg aacagcccgt
     6961 cgtgctgcgc ttgcgttcgg gctccgagct gcgcttcgcg ctgcggcccg agacagacgt
     7021 cgacggggag attcgagcga ggttgttcgc gcgacgctcc gatggatcgg gcacggtcga
     7081 actgctgatt cgccaagagc agtcggccgc gggcgcgcta cgggtcgaga gcacagtgcc
     7141 gtcgacgtta ccggaatcgg gtgaactggt tttggtggta ggccgagcgg aaacgctgcg
     7201 gggtttgagc gccagcgcga cgctggacgt gactgaaagg tcagggcagg ctttccgttg
     7261 gcgtttcgaa cactctcttt gacgacgcat gcggatgttt ggcttgagag cgccggcatt
     7321 cttacgagcc ccaccgcggg tgcggctgtg gattgcgcta ttcgccttca cggctttcgc
     7381 ggccgcacgt gatgcgcgcc ctccgcgcgc agatcatgtg ctgatgttcg agtacgctgg
     7441 gtgcgcgcgc gtgcgcccgg gcccgcgctg tgagctcggc ccggaacgtg agctgacgct
     7501 ctgggtgccg ggaaagcgtt cgctcgcgtt tgcggcgccc ggtgcgcccc cggtgagcgt
     7561 tcacgaatca cgtttcatcg agggtgggca gcgcacgcgc gttcgagtgc ccggtggcgc
     7621 aaaggtgctc cagattcgcg cgggggaatc gcaggccgag ctgctcgtgg cggagagctc
     7681 ggagcctgcg ccactcagtg agctcaggcg gctgtggcag gccggaaaat gggaagaggt
     7741 gaaagggcga ctcacggaag tggagtctcg gctgagcacc gtggagcgct ctcggctgca
     7801 ggcgtttaga ggacgactgg cgttacgcgc gggggagcac gaacgggccg ccgcagagct
     7861 cgaagcggcc gcaaagagcg cgcgttcggc cgggctttgg ctcgaagcca gcaacgatct
     7921 cttggccgcg gcgtactgcc gcgccgctcg gctgctgcag ttcgagcggg cacgggcgct
     7981 actagatgga ctgcctcggg agctcgccca agtgccggag acgaaggcgc gcatccccta
     8041 ttacctcggg gtgatcgccc ggactcgcgg agatgttcaa ggcgcactct tgcagttcag
     8101 aagggcaagt gtgtttgcgc accggctggg tctcgcgtcc gaagagggaa tggcccgaca
     8161 agaactggcg gtggtgctga acctgctcgg gcgtcgcgcg gaagccctcg ccgagcagcg
     8221 cgagctggcc gtgcatgccg agcgtgagcc caactgcttg cgcccgatcc gctgggaaaa
     8281 cctagcctgg atgttgct
//
LOCUS       DASTKU010000044         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_230430, whole genome shotgun sequence.
ACCESSION   DASTKU010000044 DASTKU010000000
VERSION     DASTKU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230430"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFQ61_01470"
     CDS             complement(<1..757)
                     /locus_tag="VFQ61_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9953138.1"
                     /translation="MKRILLVDSDPVARRPICDRLAADGHVVLEFESAAQASASPETS
                     VDAILLCHAQNSVAPENTIPGQMPAVQALRELSGLYAGVPVIVVCSTAEETLHALRQG
                     AYYVTRPPLGPDEASLLVSRALSEDERMKRVPESAPAQSMIRLNPVLATAQKASESGA
                     DEGAVMIGESAKMKAIRRVVERLGERPTTTVLITGESGSGKDTIARVLHAQTSQGGRF
                     IDISNLNAPEAALESLLFGVEAAGEEVRPGLLEQ"
     gene            complement(1044..>3343)
                     /gene="treY"
                     /locus_tag="VFQ61_01475"
     CDS             complement(1044..>3343)
                     /gene="treY"
                     /locus_tag="VFQ61_01475"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HET9953139.1"
                     /translation="FDVEFDGPTERSKDKVLLPILGSHYGRALEAGQIQVQLSPQGLS
                     VSANGRRLPCSPSSVAGLLQAAASRAHVVSELEILASSFKTVADETHPERRQRHLAVL
                     KRQLATLIAEQPSVESELASVLQEVNSDPDRLDQFLIQQYYRLAYHRIARYDLDYRRF
                     FDIDDLAALSTHRPEVFQATHELILKWVKDGELSALRVDHPDGLLDPIEYFKRLKAAS
                     HSLWVVAEKILQPGEQLPDSFQVSGTTGYDFLNALGGVFVDQSQEAAFSELYRDFLKL
                     PEAPQFANIALDAKRFALEDLLGSEVSRLTRLFQRVASSKRRHRDFAGEELRRALCEV
                     IACLPIYRTYVRPNSTPTEADLNYIRRAVQQAAKQRPELDSELMQLLEGMLSGQCAST
                     LEWEFVGRFQQISATAMAKGLEDTAFYRYFRLACLNEVGGDPARFGASPREFHAFCAE
                     LQRRWPGTLVTTTTHDTKRSEDARLRLSALTEFTSEWRTAVNEWSELARSAPGVEAPE
                     RGMEYLLWQTLVAAHPISEARLTAYLEKAMREAKRETSWANPNADYEGRILQFARGVL
                     RNADVMTSLDAFVRRISPVALRACLSQTLLKLTACGVPDIYQGSELLDTRLTDPDNRA
                     PVDFPLRRQLLEAASRASVEEALAHPEPGFAKLWMIQRVLGLRRMMPDWFGPSATYQP
                     LELHGAQAERVLAFARGQNVVVVAPRLWRSVLDHGFGDTHLPLPPGNYRNLFESELRF
                     EGRVELAGLLRRFPVALLIAERPRG"
BASE COUNT          622 a         1011 c         1084 g          626 t
ORIGIN      
        1 cttgttcgag cagccccggg cgcacctctt cgccggccgc ttccacgccg aagagcaggc
       61 tctctagcgc ggcctcgggg gcgttcaagt tcgaaatgtc gatgaaccgt ccgccttgac
      121 tcgtctgcgc gtgcagcacg cgggcgatgg tgtctttccc agaaccactc tcgccagtga
      181 ttaataccgt agtcgtgggg cgctcaccga gacgctcgac cactcggcga atcgccttca
      241 tcttggccga ttcaccgatc atgacggcgc cttcgtccgc cccggattcg gacgcttttt
      301 gcgcggtggc cagcaccgga ttcagccgaa tcatggactg ggcgggcgca ctctccggga
      361 cccgcttcat acgctcgtct tcgctcagcg cgcggctgac gagcagcgag gcctcgtcgg
      421 gtcccagtgg ggggcgcgtg acgtagtagg caccttggcg gagcgcgtgg agcgtctcct
      481 ccgcggtgct gcagacgacg atgacgggaa cacccgcata caatccagag agttcgcgca
      541 aggcttgcac ggccggcatt tgaccaggaa tcgtgttttc aggcgccacg ctgttttgag
      601 catgacagag cagaattgcg tccacgctcg tttccgggct ggcacttgcc tgcgctgcag
      661 attcgaactc taaaacaaca tgaccatctg ccgccaaccg atcacagata gggcgacgag
      721 ccaccggatc tgagtcgacg aggagaatcc gtttcatccc gacctccgac atacccgcga
      781 aaaatactct gcgaaaggag gttttaacgg cgcagactga gtgcgggtaa ctcagatgtg
      841 ccgcaatgcc gcgcgtcgag atgcagagaa cgtgccgagc ttccccacct tggggcaatt
      901 cgatcgtttt gcggcgaact tcgcctgttg aagcttcagg ttcagaggcc gacttggcag
      961 atgtgtgtga ggcgtgttcg cgaagtgggg catttgaact agctcggttg tgagcggaag
     1021 gtcactatgg gccggtagcc gggtcagcct cgcgggcgct ctgcgatgag gagcgccacc
     1081 ggaaagcggc gtaagagacc ggcgagttct actcgtccct cgaagcgcag ctcggattcg
     1141 aacagatttc gatagttgcc cggagggagc gggaggtgag tgtccccgaa accgtgatcg
     1201 aggacgctcc gccacaagcg gggcgccaca acgaccacgt tctggccccg cgcgaaggcg
     1261 agcacgcgct cagcctgcgc tccgtggagt tccaacggct ggtaagtcgc gctgggtccg
     1321 aaccaatcgg gcatcattcg acgcagcccg aggacgcgct gaatcatcca caatttcgcg
     1381 aaccccggct ccggatgggc gagcgcctcc tcgacgctcg cgcggctagc cgcttcgagc
     1441 agctgccgcc gaagaggaaa gtcgaccggg gcacgattgt ctggatcggt gagccgtgta
     1501 tccagtagtt ccgagccttg atagatgtcc ggcaccccgc aggccgtcag tttcaagagt
     1561 gtctgagaga gacaggcccg cagcgcgacc ggggagatgc gcctcacgaa cgcatcgagg
     1621 ctcgtcatga cgtccgcgtt tcgaagcacg ccgcgcgcaa actgcaggat acgtccctca
     1681 tagtcggcgt ttgggttagc ccaagacgtt tcgcgtttgg cctctctcat ggccttttcc
     1741 agatatgccg tcagccgagc ctcgctgatc ggatgggcgg caaccagcgt ctgccacagg
     1801 agatactcca ttccgcgttc tggggcctcc acgcccggtg cgctccgagc caactcggac
     1861 cactcgttga cggcggtgcg ccattcgctc gtgaactcgg tcaacgcact cagcctcagc
     1921 cgcgcatcct cggagcgctt cgtatcatgg gtggtggtgg tgacgagcgt gccgggccac
     1981 cggcgctgca gctcggcgca aaatgcgtgg aactcgcggg ggctcgcgcc gaagcgagca
     2041 gggtcaccgc caacttcgtt gaggcaggcg agccggaaat agcggtagaa agccgtgtct
     2101 tcgaggccct tggccatggc cgtggcgctg atctgctgga agcgccccac gaactcccat
     2161 tcgagggtcg atgcgcactg accactcagc atcccttcga gcagctgcat cagctccgaa
     2221 tcgagctcgg gccgctgttt cgcggcttgt tgtacggcgc gacgaatgta attcaggtcg
     2281 gcttcggtgg gcgtgctgtt ggggcgaacg taggtccgat agattggcag acaggcgatc
     2341 acctcacata gcgcccggcg tagctcctcg cctgcaaagt cacggtggcg tcgcttggag
     2401 ctcgcgacgc gctgaaagag ccgcgtgagc cgagagactt ccgagccgag caggtcttct
     2461 agcgcgaagc gcttcgcgtc caacgcaatg ttggcgaatt ggggcgcttc gggcagcttc
     2521 aagaagtctc ggtagagctc cgaaaatgct gcttcttggc tctggtccac gaagacgccg
     2581 ccgagcgcgt tcaagaagtc gtagcccgtg gtgccggaga cttgaaagct gtcgggcagc
     2641 tgctcgccgg gttgaaggat cttctcggcg accacccaga gcgagtgcga agcagccttc
     2701 aggcgcttga agtactcgat tgggtccagg aggccatcgg ggtgatcgac tcgcaaggcg
     2761 gagagctccc cgtccttgac ccacttcagg atcaactcgt gtgtggcctg aaacacctcc
     2821 gggcgatggg tagatagggc ggccaaatcg tcgatgtcga agaagcgacg atagtccaag
     2881 tcgtagcggg cgatacggtg gtaggcgaga cggtagtact gctggatcag gaactgatcg
     2941 aggcggtcag gatcggagtt cacctcctgc agtacactgg cgagctcgct ctccacgctc
     3001 ggctgctccg caatcaaggt agcgagttgc cgcttcaaga ccgctagatg tcgttgcctg
     3061 cgctcggggt gcgtctcgtc cgcgacggtt ttgaacgagc tcgcgaggat ctccagttcg
     3121 ctcacgacgt gcgcgcggct ggccgccgct tgcaggaggc ccgctacgct gctgggtgaa
     3181 cagggtaaac gccgaccgtt ggcggagaca gagaggcctt gaggcgagag ctgaacctga
     3241 atctggccgg cttcgagcgc gcggccgtag tgtgagccta gaatcggcag caacacctta
     3301 tccttcgagc gctccgtggg gccgtcgaac tcgacgtcga agg
//
LOCUS       DASTKU010000045         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_8146,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000045 DASTKU010000000
VERSION     DASTKU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8146"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFQ61_01480"
     CDS             complement(<1..185)
                     /locus_tag="VFQ61_01480"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET9953140.1"
                     /translation="MTLEQRATLLEAARKVSRHAHAPYSGYHVGAALLTRSGALFTGC
                     NVENASYGATLCAERSA"
     gene            complement(182..1045)
                     /locus_tag="VFQ61_01485"
     CDS             complement(182..1045)
                     /locus_tag="VFQ61_01485"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET9953141.1"
                     /translation="MTRSLSEPGNPQNGAEILAARDHLRPFLAEGPVVGIVLGSGLGA
                     FADHLDAPTSLLMSEIPGFPVPAVAGHSGKVVLGRLGSVGVACLAGRAHAYEGHPMER
                     VVFGVRVLAALGCGAVLLTNAAGGIRSDLSPGDLMLISDHLNLTGRNPLVGPAPSSGP
                     RFPDMSAAYDPRWAELAREAATQLNLDLKEGVYAGVLGPNYETPAEIRMLRGMGADAV
                     GMSTVLEVIALRQLGIRVSALSTITNLAAGISEHPLSHAEVEATAARVRPRFVEFLRR
                     WATLTGLELSR"
     gene            1244..1579
                     /locus_tag="VFQ61_01490"
     CDS             1244..1579
                     /locus_tag="VFQ61_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953142.1"
                     /translation="MASKFSEFLEQNKIDPRRLRAASRKLEGLRPEDRALRLAKRNAK
                     KAEGGGADKGGEAKKKPRSGRPVTFRQLDAAKAGKPISGPAKTRLVRAVNAVLTQKKK
                     DSVDLRALF"
BASE COUNT          409 a          687 c          711 g          348 t
ORIGIN      
        1 atcgcggaac gctcggcgca gagcgtggct ccgtagctcg cgttctcgac gttgcagccg
       61 gtgaacagtg cgccgctccg cgtgagcagc gcggccccga cgtgataccc agagtaagga
      121 gcgtgagcgt ggcgactgac ctttcgagct gcttccaaaa gtgttgcacg ctgctccaaa
      181 gtcaccggct gagctccagt cccgtgagag tagcccagcg cctcagaaat tccacgaatc
      241 tcgggcgcac gcgcgcggcc gtggcttcga cctccgcgtg gctcaggggg tgctcggaaa
      301 ttcccgcggc taagttggtg attgtgctga gcgctgaaac acggatcccc agctggcgca
      361 gagcgatgac ttctagcact gtactcatgc ccaccgcgtc ggcgcccatg ccgcgaagca
      421 tgcggatttc ggcgggagtt tcgtagttgg gtccgagcac accggcatag acgccttctt
      481 tgagatcgag gtttagctgg gtggcggcct cccgcgcgag ctccgcccag cggggatcgt
      541 aggcggcgct catgtctgga aaacgaggcc cgctgctggg tgcaggcccc actagcgggt
      601 ttctgcccgt aaggttcaga tggtcggaga tgagcatgag atcgccgggg ctgagatcgc
      661 tgcggatccc cccggctgcg ttggtgagga gcacggcacc gcaaccgaga gcggcgagca
      721 cccgaacccc gaagaccacg cgctccatgg gatgcccctc gtacgcatgc gcgcgacctg
      781 cgaggcacgc gacacccacg ctgccgagtc gcccaagtac gacttttcca gagtgacccg
      841 ccacggcggg cacggggaac cccggaatct ccgacatgag gaggctggtc ggggcgtcga
      901 gatgatcggc aaaggctccg agccccgagc ccagcacgat ccctaccacc ggcccttccg
      961 ccaagaaggg ccgcaggtga tcgcgggccg ccaggatttc tgcaccattt tgcgggtttc
     1021 cgggttcact caaggatcgc gtcatgagcc gtcccgtccg cggtaggccc cacgggtggg
     1081 ctcccactga ggcgattgag cgcggccgag gtggtcccgg tcacgacacc ctgaaccctg
     1141 gctcggaccg gtgcgcttta cgggcacccg acctgtgact atggtatgcg gccccgggct
     1201 ggggcagacg gacctgctcc gcccaatcaa cgaaactcga cccatggcca gcaagttttc
     1261 agaattcctc gagcagaaca agatcgaccc tcgccggctg cgcgccgcat ctcgcaagct
     1321 ggaaggcctg cgacccgagg accgcgcgct tcgcctggcc aaacgcaatg ccaagaaggc
     1381 ggaaggcggc ggcgcggaca aaggtggtga ggccaagaag aagccccgct cgggccgccc
     1441 cgtgactttc cgtcagctgg acgccgcgaa ggcgggcaag cccatttccg gaccggccaa
     1501 gacccgcttg gttcgcgctg tgaacgcggt cttgacgcag aagaagaagg acagcgtcga
     1561 cctgcgcgcg ttgttctagg attgagagcc gcctcataag gctcttgacc agcagcgctc
     1621 aggcagagtt ttcgaaatcg acgtgtgaca tcagaaatgt gcctgcggtt ttgaaaacac
     1681 cggggagtgt aggcatccaa cccgttcgtt gatgcttgcg ctcctcgtgc cgacgaagtc
     1741 tctgacgccc gtctcccgcg tgtcattgat cccacgatcg acgctgcacg aagtagggcc
     1801 attgccgtca aaggacgttc ccccggggcg cgcgtctacg gaatacgggc tgactcggcc
     1861 ggggcagacc gagcccggcg gcgggcaaac agagcgcggg caaacagagc gcgggcaaac
     1921 agagcacagc gggatacatg cactgccacc gtcccatggc ggcgcgcgca cgagctcgcc
     1981 caaagggctc tcgagaattg aaagcgagaa aaccgggcct cgtgtgacga ccggcgctat
     2041 tctcgagcgc gtgatcgagc ccgaagagct cgttcggcgt tgccaagcgg gcgatcagga
     2101 agcttttaga gagctgttcc ggcgccatgg cggtgacgtg gcgcggctcg tacag
//
LOCUS       DASTKU010000046         6796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_230721, whole genome shotgun sequence.
ACCESSION   DASTKU010000046 DASTKU010000000
VERSION     DASTKU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230721"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2088
                     /locus_tag="VFQ61_01495"
     CDS             <1..2088
                     /locus_tag="VFQ61_01495"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET9953143.1"
                     /translation="GADVVLVEGGLSSFPLRGRADKQGLIRLGPISRAPAVITAQAED
                     FVGAPLLAVPDVLPEPPLRLALTRGGRLRGRVVDSRDIGIPGASVEVVGTDRFGLPID
                     VTPLTVGFRSSHFEWALTGPQSLIPAGELGVMPGPLPTAHPPPPATPLLQGLGTPNLA
                     PGLPGAGLGAPFPAPDARAASASSDLVTRAASISSNPASWSSDVHGEFEARPIPPGSV
                     RALVRHPEYVEGQSELVNVGPGASAEVRIVLLHGGTLEGFVKDARGFPVGGVQVRVQA
                     QRGPFERSTFSASDGSFAFSAVPEDVILQALRPDAESRVVVRKSVTVRDGARERVELT
                     LPEARDPIIITVQGPRDAALELVELRVSSLDPNNPLRSTLFTDANGRAQLADALGLPL
                     RIAADAPGYARVEQTVDSAPAELSVRLSEGIALTGRITAVRGRQSVVSAVVTLIQGTQ
                     RKSTTSNGDGLFKFRDVAPGAIEIHVQHPDYAPGLLKAQATATGRSDRPLELDTIDLL
                     EPGELEGTVEDARGQPVEGARVALGASPSVLPAGVIPEGIALTDAQGQFKLRRVAPGK
                     AQLSAYAPNLGRGTLENVEVVAGRTAMGLRLRLTQAPTKDENGESSGVAILVSQRAAD
                     VVISEVARGSEAERAGLLIGDLVRAVDGVRPERAEQARERLAGRAGSDVLLELERQGQ
                     LLRLRVGREELRR"
     gene            complement(2094..2882)
                     /locus_tag="VFQ61_01500"
     CDS             complement(2094..2882)
                     /locus_tag="VFQ61_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953144.1"
                     /translation="MSCSRVVCAGWMVVCLWAAGCKEKQPENLAPVASALVSAPKPAE
                     AIKLTIDKASSKLEFLMEAPIEKISGEADAAVEGELFVDTADLSKSTGLVRADLNKLV
                     LYQEKREDEKGQFGARAKNDLQNQHARNWLEISDDVPEQTRADNANVEYRISRLENPS
                     INDLSRAPGTERKVTATAVGDFRLHGRKNEKRAKIELIFTYANDKLASVRVSSLEALP
                     IGLDEFDVRPREAFGKLAQKTLSALGSKVAQSAPIRVSFTARAE"
     gene            complement(3135..3275)
                     /locus_tag="VFQ61_01505"
     CDS             complement(3135..3275)
                     /locus_tag="VFQ61_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953145.1"
                     /translation="MEPGLVTLAKIIGTLLTAMGMIPVLFLLSGGDTTEPQDRLDSEF
                     KG"
     gene            complement(3321..4493)
                     /locus_tag="VFQ61_01510"
     CDS             complement(3321..4493)
                     /locus_tag="VFQ61_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET9953146.1"
                     /translation="MTAAALSVLSEDERLFLDTVSSFAEARIKPYVAEMDERAALEPE
                     IVRTLFDLGVMGIEIPPQYGGAGSNFFTAILAVQALSRVDPSVAVMVDVQNTLVNNAL
                     LRFGTEEQKGRYLPKLANSWVGSYALSESSSGSDAFALRTRAERRGDGWVLSGRKMWI
                     TNAAESSLFIVFANVSPEKGYKGITAFLVERGFPGFSIGKKEEKLGIRASSTCELVLD
                     ECAVPGENVLGAVGQGYKIAILTLNEGRIGIGAQMLGLAEGAFELSMAYLNERKQFGQ
                     AIAEFQGVQFQYARLAMELQAARLMVYDAARLKDGGTEFVKQAAMAKLFASEVAERAA
                     SQAIEFFGGVGFTREYPVEKLYRDAKIGKIYEGTSNMQLQTIAKLLQVERRGSSDT"
     gene            4644..5777
                     /gene="aroC"
                     /locus_tag="VFQ61_01515"
     CDS             4644..5777
                     /gene="aroC"
                     /locus_tag="VFQ61_01515"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET9953147.1"
                     /translation="MPGNSFGQAFRVTTAGESHGPGNVVIIDGCPPGLALSVEDLRGD
                     LQRRRPGQSHIVTQRAEADEPEILAGVFEGRTTGTSLAILIRNTDQKSRDYADIQDKY
                     RPGHADHSYDAKYGFRDYRGGGRASARETVARVAAGAVAKKLLAEAYGTRIVGYVRQI
                     GDLVAHLPDPSSITPEQVERLPDGTPNLVRCPDPVCAQAMVELIETVRKDQDSIGGVG
                     EVVASSVPAGLGEPVFDKLKADLAKALFSVPAVMGVEYGLGFACAGLRGSASNDAFET
                     DGGRIVTRTNRHGGMLGGISSGMPIVLRAAVKATSSLPREQETVTRDGSPTTIRTRGR
                     HDPCLLPRFVPIAEAMVALVLADHALRQRGQRGVTSTSHGEGL"
     gene            5774..6595
                     /locus_tag="VFQ61_01520"
     CDS             5774..6595
                     /locus_tag="VFQ61_01520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9953148.1"
                     /translation="MKKLHYPGAWALITGASSGLGAEFARQLAHRGSNLVISARNQQR
                     LQQFGDDLRRVNGVEVRAVAADLSTSDGITGLLNAVDDLKVPIRHLINNAGFGSVGAF
                     ADGDAEQERRMLHVNVEAVVALSRHFLPRLLAVAEGGIIQVASTVAHQPTPFMATYGA
                     SKAFVLNFALALSEEVRGSGVRVLTLCPGPVPTGFQAAAGFETTGLMRLTKLEASEVV
                     ERALAAYEDGKRIVTPGVLNAAQTRAVKVLPEPVVLRAARWAMQSLGRTTRRNSE"
BASE COUNT         1186 a         2212 c         2200 g         1198 t
ORIGIN      
        1 ggcgccgatg tggtgctggt ggagggcggg ctgagctctt ttccactgcg cggacgagcc
       61 gacaagcagg gactaatcag gctgggccca atttcccgcg cgcccgcggt gatcacggct
      121 caggccgagg acttcgtggg cgcgccgttg ttggcggtcc cggacgtgtt gccggaaccg
      181 ccgctccgct tggctctgac gcgtggcggt cgattgcgcg ggcgtgtggt cgactcgcgc
      241 gacatcggca tcccgggcgc gagcgtggaa gtagtcggga ccgaccgttt tgggctaccc
      301 atcgacgtca cgcctttgac cgtcggcttt cgctcatccc atttcgagtg ggcgctgacg
      361 ggcccgcaaa gcttaatccc cgcgggtgaa ctcggggtca tgccggggcc gctgcctacg
      421 gcgcatccac cgccgccggc aacgccactc ttgcagggct tgggcactcc aaacctcgct
      481 ccaggcctgc ccggtgcggg gctcggcgct ccatttccag cgcccgacgc gcgtgccgca
      541 tcggcgtcga gcgatctggt gacgcgtgcg gcctccattt catcgaaccc cgcgagttgg
      601 tcgagtgacg ttcacggaga attcgaagcg cggcccatcc ctcccggttc ggtgcgggcg
      661 ctggtgagac atcccgagta cgtcgaaggt cagagcgagc tcgtcaacgt tggtccgggc
      721 gcgagcgccg aggtgcggat cgtgttgctg catggtggga cgctggaggg cttcgtcaaa
      781 gacgcgcgcg gctttcccgt gggcggcgtg caagtccgcg tacaggcgca gcgtggacca
      841 ttcgagcgca gcaccttcag cgccagcgac ggctctttcg cgttttccgc cgtaccagaa
      901 gacgtaatcc tgcaggcgct gcggccagac gccgagagcc gcgtggtcgt gcgcaaaagc
      961 gtgaccgtgc gggatggggc acgcgaacgt gtggagctaa ccctgcccga agctcgtgac
     1021 ccgattatca tcaccgtgca gggtccgcgt gacgccgcgc tcgagctcgt ggagctgcgc
     1081 gtctcctcgc tcgatccgaa caacccgcta cggagcacgc tctttacgga cgcgaatggc
     1141 cgcgcgcagc tcgcggacgc cctcggcttg cctttgagga ttgccgccga cgccccaggc
     1201 tacgcgcgcg tagagcagac cgtagatagc gcacccgcgg agctgagcgt gcggctgagt
     1261 gaaggtatcg cgctcacggg gcgcatcact gcggtgcgag gccggcaatc cgtcgtttca
     1321 gcggtggtca cgctgatcca agggacgcag cgcaagagca cgacgagcaa cggggacggc
     1381 ctcttcaagt ttagggacgt ggcgcccgga gccatcgaga tccacgtaca gcatcccgat
     1441 tatgccccgg gactgctgaa ggcacaggcg accgccaccg gccgcagcga ccgccccctc
     1501 gaactcgaca cgatcgatct cctcgaaccg ggtgaactcg aggggacggt ggaggacgcg
     1561 cgcggacagc cagtcgaggg ggcacgcgtg gcgctgggag cgtcgccgag cgtgctgccg
     1621 gccggggtta ttcccgaggg gatcgcactg acggacgcgc agggtcagtt caagctccgc
     1681 cgtgtcgctc ccggcaaggc gcagctcagt gcttacgctc ccaacttggg gcgcggtacg
     1741 ttggagaacg tggaagtcgt ggcgggccga acagccatgg gtttgcgcct gcgtcttact
     1801 caagctccga ccaaggacga gaacggcgag agctcggggg tggccatcct cgtctcccag
     1861 cgagccgccg acgtcgtgat tagcgaggtg gcccgcggga gcgaagccga gcgcgcgggg
     1921 ctgttgatcg gcgatctcgt tcgagctgtc gacggggttc gccctgaacg cgccgaacag
     1981 gcgcgggagc gcttggcggg ccgcgccggt agcgacgtct tactcgaact cgagcgtcaa
     2041 gggcagctgc tgcgcttgcg cgtggggcgc gaagagctgc gccgctgatg ggtctattcg
     2101 gcgcgcgccg tgaaggacac acgaatgggc gcgctctgcg ccaccttgga cccgagtgca
     2161 ctcaaggtct tctgagcgag cttgccgaat gcttcgcgcg gccgcacgtc gaactcgtcg
     2221 agcccaatcg gaagggcttc gaggctcgac actcgcacgc tcgcgagctt gtcgttcgcg
     2281 tacgtgaaaa tcagctcgat cttggcgcgc ttctcgttct tacgtccgtg cagacggaaa
     2341 tcaccgacgg cggtggcggt taccttgcgc tcggtgccgg gcgcgcgcga gagatcgttg
     2401 atggaagggt tctcgagccg cgagattcga tactcgacgt tggcgttgtc cgctcgcgtc
     2461 tgctccggaa cgtcgtccga aatctcgagc cagttgcgcg cgtgttggtt ctgcaaatcg
     2521 ttcttggcgc gcgccccgaa ttggcctttc tcgtcctcgc gcttctcttg gtagagcacg
     2581 agcttgttca agtcggcgcg aaccaagccc gtagacttcg agagatccgc ggtgtccacg
     2641 aacagctcgc cttccacggc cgcatccgct tcacccgaga tcttctcgat cggcgcttcc
     2701 atcaagaact cgagtttgct cgaagccttg tcgatcgtga gcttgatggc ctctgctggc
     2761 ttgggcgccg acaccaaagc cgacgccact ggtgccagat tctctggctg cttctccttg
     2821 caacccgcag cccacagaca cacaaccatc caacccgcgc aaaccactct cgagcacgac
     2881 atagtattcc cttccggcca cgcgacggcg cgctccctga tgaagcgtac atcctacgcg
     2941 ggcatccaag ctacgccgct aacgccccct ggtttcattc cagaccggca cttttctcca
     3001 accagcgcgc tccgtttccc tacggccgag tttccctacg gccgagtttc cctgcggccg
     3061 aatttcccct cagccgagtt ccccttcggc cggcaccgag gctcgaggcg ccggcgccgc
     3121 ccacggctgg ctgatcagcc cttgaactcg gaatcgagcc ggtcttgcgg ctccgtggtg
     3181 tcaccaccag agagcaagaa caggactggg atcatgccca tggccgtcaa aagggttccg
     3241 atgatcttgg cgagcgtaac gaggcccggt tccatagtga gccggacgat agtccgagag
     3301 gcctaactgg caagaagatt ttacgtgtcc gagctgcccc gacgctctac ctgcaacaac
     3361 ttggcgatgg tctggagctg catgttgctc gttccctcgt aaattttgcc gatcttcgcg
     3421 tcccgataca gcttctccac aggatactca cgggtgaagc cgaccccgcc aaaaaactcg
     3481 atggcttggg aggccgcgcg ttcggcaacc tccgaggcga acagcttggc catggcggcc
     3541 tgcttgacga actccgtgcc cccgtccttc aggcgcgcgg catcgtagac catcaagcga
     3601 gcggcctgta gctccatggc gagccgcgcg tactggaact ggaccccctg gaactccgcg
     3661 atcgcctgcc caaactgctt acgctcgttc agataagcca tcgagagctc gaacgcgccc
     3721 tcggccaggc ccagcatctg ggcgccgatt ccgatacgcc cttcgtttag ggtgaggatc
     3781 gcgatcttgt agccctgccc gaccgccccg agcacgttct cgccgggcac tgcgcactca
     3841 tcgagcacta actcgcaggt gctcgaggcc cgaatcccga gcttctcttc ctttttgccg
     3901 atgctgaagc cggggaagcc ccgttcaact aggaacgccg tgatgccttt gtagcccttt
     3961 tccggggata cattggcaaa cacgatgaag agcgagctct ccgccgcgtt ggtgatccac
     4021 attttgcggc cgctcaacac ccagccgtcg ccacgacgtt cggcacgcgt gcggagcgca
     4081 aaggcatcag aaccgctgct ggactcactg agcgcatagg agcccaccca agagttggcg
     4141 agcttcggga gatagcggcc cttttgttcc tccgttccga agcgcaacag cgcgttgttc
     4201 acgagcgtgt tttggacatc gaccatcacc gcaacactcg gatcgacgcg cgagagcgct
     4261 tgaacggcca gaatggcggt gaaaaagttc gagcccgcgc cgccatattg cgggggaatc
     4321 tcgatcccca tcacccccaa gtcgaatagc gtccgcacga tctcgggttc gagcgccgct
     4381 cgctcgtcca tctccgcgac gtagggtttg atgcgcgcct ccgcgaacga agacacggtg
     4441 tcgaggaaca agcgctcgtc ttcggagaga acggacaatg cagcggctgt cacgcgcttc
     4501 agactagcat cggcggtggg gttttcggag cggatgcgcc atgcgcccca agcgccgcca
     4561 aatccgcgca aaaagcggct cggccagcgc cgcccgaccc ccggctaccg tgggcgccac
     4621 tccgtggtaa aggggcaggc tcaatgcccg gcaacagttt cggtcaagct tttcgtgtaa
     4681 ccacggccgg cgagagccac ggccccggga acgtcgtcat catcgacggt tgccctcccg
     4741 gtctcgctct ctcggtggag gacttgcggg gggatctcca acgccgccgc cccggtcaaa
     4801 gccacatcgt gacccagcgt gccgaggcgg acgagccgga gatcttggcg ggcgtgttcg
     4861 agggccgcac gacagggacg agcctcgcga ttctaatccg gaacaccgac cagaagagcc
     4921 gcgactacgc cgatatccaa gacaagtatc gccccggtca cgccgaccat agttacgacg
     4981 ccaagtacgg cttccgagac tatcgaggcg gaggacgagc aagcgcccgc gaaacggtgg
     5041 cccgagttgc ggccggcgcg gtcgcgaaga agctgctggc ggaggcttat ggcacgcgca
     5101 tcgtaggcta cgtgcgacaa atcggggacc tggttgctca cctgccagac ccgagcagca
     5161 tcacccctga acaggtcgaa cgcttgcctg atggtacgcc gaacctagtg cgctgcccgg
     5221 acccggtatg tgcccaagcc atggtcgaac tgatcgagac ggtgcgcaaa gaccaagact
     5281 cgatcggggg tgttggagag gtcgtggcca gctcggtgcc ggcggggctc ggcgagccgg
     5341 tgttcgacaa gttgaaggcg gacctggcca aggcgctgtt cagcgtcccc gccgtgatgg
     5401 gagtcgagta tgggctgggc ttcgcctgcg cggggctgcg cggcagtgcg agcaacgacg
     5461 ctttcgagac agatggcggc cggatcgtga cccgcaccaa tcggcatggc ggcatgttgg
     5521 gcggcatttc ctccggcatg ccgatcgtgc tgcgggcggc ggtgaaggcc acgagtagcc
     5581 tgcctcgcga acaggagacc gtaactcgtg acggcagccc aacgacgatc cgcacgcgcg
     5641 gtcgtcacga tccgtgtcta ttgccgcgct tcgtcccgat cgcggaggcg atggtggcgc
     5701 tggtcttggc cgatcacgct ttgcggcagc gcggtcaacg cggagtcacg agcacgagcc
     5761 acggcgaggg gctttgaaga agctgcacta tcccggtgcg tgggcgctga ttacgggggc
     5821 ttcttcgggg ctcggcgccg agtttgcccg ccagctcgca caccgcggga gcaacctggt
     5881 gatcagcgcg cgcaatcaac agcggttgca gcagtttgga gacgacttgc ggcgcgtcaa
     5941 cggcgtcgag gtacgcgccg tcgccgccga cttatccacg agcgacggca tcactgggct
     6001 cttgaacgcc gtggatgatt tgaaagtgcc aattcggcac ctcatcaaca atgccgggtt
     6061 cggttcggtg ggtgcattcg cggacggcga cgccgagcag gagcgacgca tgctgcacgt
     6121 caacgtggaa gctgtcgtgg ctttgagtcg gcacttcttg cctcgcctcc tcgcggtcgc
     6181 agaggggggc atcatccaag tagcgtcgac ggtcgctcac caaccgacgc ccttcatggc
     6241 cacttacggg gcgagcaaag cgttcgtcct gaacttcgcc ttggccctct ccgaagaggt
     6301 tcgtggtagc ggcgtgcgcg tactgacgct gtgccccggt ccggtaccga ccggtttcca
     6361 ggcggccgca ggtttcgaga ccacgggctt gatgcggctc accaagctcg aggcgtccga
     6421 ggtggtcgaa cgagcactcg ccgcttacga ggacggcaaa cgtatcgtga ctccgggcgt
     6481 gctcaatgca gcgcaaactc gcgccgtcaa agtcctgccc gagcccgtcg tgctacgagc
     6541 ggcgcgctgg gccatgcaga gcctcggacg caccacccgc cgcaactcgg aatgagcggt
     6601 acagaacacc gctcatccgc tccctcactc gaccgagctc agccaagcat cgaagaagcg
     6661 ccgagcacgc tttgccaact cgggcgcata tcgatgggct tcagagcgta gatcgccgat
     6721 cgtgagcccg ggtgtcgcgc agatctcgtt gatgtgcccg atgtaccaac gctcgagccc
     6781 gcgcgccgtg acctcg
//
LOCUS       DASTKU010000047         7512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_230918, whole genome shotgun sequence.
ACCESSION   DASTKU010000047 DASTKU010000000
VERSION     DASTKU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230918"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFQ61_01525"
     CDS             complement(<1..530)
                     /locus_tag="VFQ61_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953149.1"
                     /translation="MAAADAGTHRYLGFATFSEYVERLFGYEPRTVAEKLRVARALVH
                     LPELSAAFSSGVLNWSAVRELTRVATAKTENEWLSATVGLSARQVQERVRGRKLGDTP
                     LAQPDPSRIRHTLRFDVCAEVLATFREALAKLRRDSGEALLSDDDVLLLLARRVLDEP
                     RQEEGAPRQSDDQGVA"
     gene            complement(1243..2526)
                     /locus_tag="VFQ61_01530"
     CDS             complement(1243..2526)
                     /locus_tag="VFQ61_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9953150.1"
                     /translation="MHSTPTVYAIDFGTTNSLVCAANAERTWAPLSIDPGASDPSIFR
                     SLLFFPDDNPSIACGQAALSACADSGMRGRLIRSLKRFLPAQTFTETRIGTRRYALEE
                     LVAILLRTLRERADAALSTQVRAVVLGRPVRFSDDPECDALAAARLERAARLAGFDHV
                     ELCEEPVAAALDCAEQQNSSELCAVLDLGGGTSDFTIARLGAGRSDVLAIGGIAIAGD
                     ALDGSLMRERVSPYFGSTVRYRMPFGGNVLTFPKPLLEKMCSPAELSLLDRREVLEFL
                     RSIRVFSLSEEDRERMDRLLCLVEDRLGFQIFEVIDQTKRALSASLSAPLHFDYPGIE
                     LDEVVTRSEFEAAASRPIQSILERLEQTLERAGVGFEQLDRVYFTGGTARVPAIVQAV
                     SARVHPDRIQHVRTFHAVVQGLAERARQIATELAH"
     gene            2881..4341
                     /locus_tag="VFQ61_01535"
     CDS             2881..4341
                     /locus_tag="VFQ61_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953151.1"
                     /translation="MSSFHRGMYGIAMLVIGCSQSSTLGNGAPSQGGEAEDVNTPSIT
                     ELDAYSKLELALSGKPHSTPPHAGHDAHGAHEGHGAHGNGHNDGHDCEGTDAHGAEQP
                     EPIRDAAAETAQRLIALAADSIGDNAHNGLPDFDPGDGGWDFVLSSSATSHSERASPE
                     NTYGATALGLWSAPAPVRTQPRSVAALVDAYLGASRRAEVDSSPDLVWLVSMADEHQN
                     PGYAELARQRYDAARDRLGGVEAVARDLVQRRVRANADGLVTYDLAWLGLGAAALSRA
                     FPSADYANDVATVTRICAEALALTGTEPAAFDLEDRSEPYYVQGVAGALLALSLDPAT
                     RNFTPQVRRILFDEQLSDGGFGFNSAYPTANLQASAQVILALVLADPTPRAIRRAQRA
                     ARWVVSRHRAGGGFEAQAGLESPLLDAEILLALRVAYAGPRDDSRELGSVTDADDVTP
                     VDTVVASSQSALRVLGDQPSLGSPALLAAQSSVTRR"
     gene            complement(4281..5240)
                     /gene="rsmI"
                     /locus_tag="VFQ61_01540"
     CDS             complement(4281..5240)
                     /gene="rsmI"
                     /locus_tag="VFQ61_01540"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET9953152.1"
                     /translation="MPEDPAWGRPLKSSLKASAKSRPASGEETGAEGVGTLFLVATPI
                     GNLQDITLRAIETLKAVDTVAAEDTRRTRQLLSHLGITQKQLTSYNAHSGESVAARLL
                     ESLLAGANIALVTDAGTPSVSDPGTDLVGQAVRAGVPVVPIPGVSAVTAAVAVSGLVQ
                     NRFRFLGFAPQKGARRRELFEEVARSIDPCILFEAPHRAERTLGELARVCPDREAAVC
                     RELTKVHEEVRRGTLRQLAEHEAEWRGEFTIVVAGAPVRDVEHGAPPEALDEEILGRL
                     SAGETPRSIVEALEATADMPRRELYRRVTELCAASSAGEPSEG"
     gene            complement(5207..6544)
                     /locus_tag="VFQ61_01545"
     CDS             complement(5207..6544)
                     /locus_tag="VFQ61_01545"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HET9953153.1"
                     /translation="MTSPKPEKPAGRSRSPGGAQAGHAADGSSADRSAPAIEAGEAIV
                     EPRPARATVSRAGARSKTSKSERTQRTAGKVSAASKPRAQGKQREVEKASVAAGEPSE
                     SADEYADDESSESELGADSEIEVDAELDADSGNLDPDVLEATAEVLSDETVEHEPEPE
                     VDSTALAKVDALAAYMREVQRHPLLTPDREHALAVEYKKTGDVNAAASLVTANLRLVV
                     KIAYEYRRAYRNMMDLIQEGNIGLMQAVKRYDPYRGVKLSSYAAWWIRAYMLRFILNN
                     WRMVKIGTTQAQRKLFFNLSKEKARLSQMGIEPTHAEIAKSLNVEESEVMEMDRRLAR
                     QDASLDAPVTEGDDRATTRLELLPSVADSPDRIAEDAQLHGLLREKLESFRSTLTGKD
                     IAIFDKRLVADEPLTLQELGDEFGVSRERVRQLEARLTGKLREYLKTQLGDGL"
     gene            7058..>7512
                     /locus_tag="VFQ61_01550"
     CDS             7058..>7512
                     /locus_tag="VFQ61_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953154.1"
                     /translation="MFERDLVDRDAAEWIRAALDGAPFPVGICAEGRLVYENSRFSDW
                     ILEPELAAPNAPECAPELPPERSYTTRRQQVRELVADLSHVLPGPSAQRFAGPAGVGV
                     PTPLSPFPSGGPGSSRFAPVESPGLASAGVSVGVAKSATRQTRFISGGGG"
BASE COUNT         1371 a         2339 c         2356 g         1446 t
ORIGIN      
        1 ctcgccacac cttgatcgtc actctgccgc ggagcgccct cttcctgccg tggttcatct
       61 agcactcgac gagccagcag caacaagaca tcgtcgtcgc tcagcaacgc ctcgcccgag
      121 tctcgacgca gcttcgccag cgcttcacga aaggtcgcca gcacctccgc acaaacatcg
      181 aacctcaacg tgtgtcgaat gcgggatggg tccggctgcg cgagcggggt gtcaccgagt
      241 ttccgccccc tcactcgctc ttgtacctga cgcgcggaca gtcccaccgt agcactcaac
      301 cattcattct ccgtctttgc ggtcgcgact cgcgtcagct cgcgcaccgc actccagttg
      361 agaacacccg agctgaacgc tgcactgagt tcaggcaagt gcaccaaagc tcgcgccaca
      421 cgcaatttct cggcgactgt tctaggctcg tacccgaaga gccgttcgac gtactcggag
      481 aacgtcgcaa aaccgagata gcggtgggtg cccgcgtccg cagccgccat gaggacttgc
      541 ccctccaacc aatcatgctc aattttgttg ttggataacc gtctgagttc tcgatccgct
      601 ctcagccact ccggttccgg agcggcgtcg cttccctccc ggttgtatag gcgtttttta
      661 gaccgaggct ctggttcgcc aggttgcttt agaggcgtcg cccttccctc tgtaaatcga
      721 ccatcctgag ctccattggc gtgcagtgga atctcgaccg atggaatctc gaccgatgga
      781 atctcgacca atggaacccc tacctccccg aacacttcca tgactcgagc ataacacagt
      841 atattttgta ttcaagcctt acccatcaaa cacttcgaga gccatatcga ctatccaaat
      901 ccatctggcg catgccagtt acccatacga agattcacta cggaaactca gcacaccggg
      961 caaccccgtt ggttaacttc gtgggcccgc gcccgcatct tggcggccgc agtggattgg
     1021 cctcgcgaca acgtgcgcga cgctcgtcat ggtgtcaccc cggcaccggc ctgaccgcgc
     1081 acagctctcc ttataccgcg ctggcccccc gctgcagacg atccatattg aatgtcgtcg
     1141 cgcgttcaaa ttggaaagca ctcgcgccca cgtgggcatc ggggcatcgc gaacaagacg
     1201 aacaaacgac cgcagatgcg cgtccggcga gtcgcggcaa gctcagtggg caagctcagt
     1261 ggcaatctgt cgagctcgct cggcaaggcc ttgaaccacc gcgtggaaag tgcgcacgtg
     1321 ttgtatcctg tccgggtgaa cgcgggcgct gacggcctgg acgatggcag gcacccgggc
     1381 cgtacccccc gtaaagtaga cgcgatcgag ctgttcgaag cccacgccag ctcgttcaag
     1441 ggtttgctcg aggcgctcga ggatgctctg gatggggcgg gaagcagcag cttcgaattc
     1501 cgaacgagtg acgacttcgt ccagttcgat gccagggtaa tcgaagtgta gcggagcaga
     1561 gagagaagcc gagagagcgc gtttggtctg atcgatgacc tcgaagatct gaaagccgag
     1621 tcgatcctcc acgaggcaaa gcagtcgatc catgcgctcg cgatcttcct cgctcagcga
     1681 aaaaacgcgg atgctccgca ggaattccaa aacctcgcga cgatccaaaa ggctgagttc
     1741 cgcgggcgag cacatcttct cgagcaacgg tttcggaaag gtgagtacat tccctccgaa
     1801 cggcattcgg taacggacgg tcgagccgaa gtacggcgaa acccgctcgc gcatgagtga
     1861 cccatccagc gcgtcgccgg cgatcgcgat tccaccgatg gccaagacgt cgctgcgccc
     1921 ggcaccgagg cgtgcgatgg tgaagtcgga ggtgccgcca cccagatcga gcacggcgca
     1981 gagctcgctc gagttctgtt gctccgcgca atcgagcgca gccgccacgg gttcttcaca
     2041 cagctctacg tgatcgaaac cggcaagtcg cgcggcgcgc tcgagccgag cggccgccaa
     2101 cgcgtcacac tcgggatcat cggagaagcg cacggggcgc ccgagcacca cggctcgcac
     2161 ttgggtcgac agcgctgcat ctgcgcgctc gcggagcgtg cgcagtagga tggcgaccag
     2221 ctcttcgagt gcgtaacggc gagttccgat tcgggtttca gtgaaggtct gagcaggcag
     2281 gaaccgcttc agagatcgaa tgaggcggcc gcgcatcccc gaatccgcgc aggcgctcaa
     2341 tgctgcttgt ccacaagcaa tgcttgggtt gtcgtcgggg aagaacagaa ggctccgaaa
     2401 gatgctgggg tcgctcgcac cgggatcgat gctcagcggg gcccacgtgc gttctgcgtt
     2461 ggccgcacac accaaggagt tcgtcgtacc gaagtcgatg gcgtacacgg tgggagtgga
     2521 gtgcatggcg cttgtgtgct cgaaatctca ctcctttaca aggctaagaa tccgtgagag
     2581 cctgcggaag gctattggga ggtcccagcc gcttcccaac aaggatctcg atgtgccgtg
     2641 atcgggaact ccgcggttat acacgccact cggcacagcc gcatcataag gcacagccgc
     2701 cataaggcac agccgccata aggcacagcc ggcggtgcga ttcgtaacca tgggttcgtt
     2761 cattattcgg ttcgcggcca ctaaatcggc gaaggcacac tcatggattg tggtagcgcg
     2821 atgggctcgg taggtttgcg aagagggatt tgcagctcgg ctgcgagaaa ggacctgctc
     2881 atgtcgtctt ttcatcgtgg gatgtacggt attgcgatgt tggttatcgg atgctctcag
     2941 agttcgaccc taggcaacgg ggctccgagc cagggcggcg aagcagagga cgtgaatacg
     3001 ccgagtatca ccgagctaga tgcgtactcg aagctggagc tcgcgctgtc gggcaaacct
     3061 cattcgaccc ctccgcacgc tggtcacgac gcgcatggtg cccacgaggg tcacggtgct
     3121 cacggaaatg gccacaatga tgggcacgac tgcgaaggca cagacgctca cggggcggag
     3181 cagcccgagc caatccgcga cgccgcggca gagacggctc agcggctaat cgcgctggcg
     3241 gcagactcga tcggagataa cgcgcacaat ggcttgccgg acttcgatcc aggggacgga
     3301 ggttgggact tcgtgctgtc gagttcggcc accagccact cggagcgcgc gagtccggag
     3361 aatacttatg gcgctacagc attggggttg tggtccgccc ccgcaccagt tcgcactcag
     3421 ccccgctcgg tagcagcttt ggtagatgca tacttgggcg catcgcgccg agcggaggtg
     3481 gattcgtcgc cagacttggt gtggctggtt tcaatggcgg acgaacacca gaacccaggt
     3541 tacgcggagc tcgcgcgaca acgttacgat gccgcgagag atcgattggg cggagtggaa
     3601 gccgttgcgc gcgacctggt gcaacgtcgt gtgcgggcca acgcggacgg cctcgtgacc
     3661 tacgatttgg catggctggg actgggcgca gccgcgctga gccgtgcctt ccccagtgcg
     3721 gactacgcca atgacgtggc gacggtgacc cgcatttgtg ccgaggcgct ggccttgacg
     3781 ggcacggagc cggctgcgtt cgatctggag gatcgctcag agccttacta cgtgcaaggg
     3841 gtagccggag cgttgctagc gctatcgctc gatcccgcaa ctcgcaactt cacgccgcag
     3901 gtgcgcagga tcttgttcga tgagcagctg agtgacgggg gattcggatt caattcggct
     3961 tatcccacgg ccaatttgca ggcgtccgcg caagtaatct tggctctagt gctggccgat
     4021 cccactcccc gcgccatccg gcgagcgcaa cgagcggcgc gctgggtggt ctctaggcat
     4081 cgcgccggag ggggattcga agcacaggcg ggactggaga gccccttgct ggatgcggag
     4141 atcctgctcg ctttacgggt ggcatacgcg gggcctcgag acgattcacg cgagctaggg
     4201 agcgttaccg acgcggacga cgtcacccct gtcgatacgg tggtcgcgag ctcccagtcc
     4261 gctctgcgtg tgttgggtga tcagccctcg ctcggctcgc cagcgctgct cgcggcgcag
     4321 agctccgtca ctcgacgata gagctcgcgt ctcggcatat cagcggtcgc ctcgagcgct
     4381 tcgacgatgc tgcgcggcgt ctctcccgcg gagaggcgcc ccagtatttc ttcgtcgagc
     4441 gcttccggag gcgcgccgtg ctcgacatcg cgaacgggag cacccgcgac gacgatcgtg
     4501 aattcgccgc gccactcggc ttcatgctcg gcgagttgcc gtaacgtacc gcgacgcacc
     4561 tcttcgtgaa ccttggtgag ctcgcgacaa actgcggcct cccgatcggg gcatactcga
     4621 gcgagttcac ccagcgtgcg ctcggcgcga tgcggcgctt cgaataggat gcagggatcg
     4681 atcgaacgcg cgacttcctc gaatagctct cggcgccgag cgcccttctg cggcgcaaag
     4741 cccaagaacc taaatcgatt ttgcactaac ccgctcacgg ccaccgccgc cgtcaccgca
     4801 ctgacccccg gaatcggaac cacgggcacg cccgcccgca ccgcctggcc cacgagatcc
     4861 gtcccgggat cgctgaccga gggggtgccc gcgtccgtga cgagcgcgat gtttgccccc
     4921 gcgagcaggg actcgagcag acgtgcggcg accgattctc ccgaatgggc gttatacgaa
     4981 gtgagctgtt tctgagtaat cccgaggtgt gagagcagct gccgtgtacg tcgtgtatcc
     5041 tcggccgcca ccgtgtcgac cgctttcagc gtctcgatcg cgcgcagggt aatatcctgc
     5101 agattgccaa tcggagtggc gacgaggaat aaggtaccta caccttctgc gcctgtctcc
     5161 tcgccgctcg cgggtcgaga tttggccgat gctttcagag aggatttcag aggccgtccc
     5221 caagctgggt cttcaggtat tcgcggagct tgccggtgag gcgcgcctcg agctggcgca
     5281 cgcgttcacg cgagacgccg aattcgtctc ccagctcttg cagagtgagt ggctcgtcgg
     5341 ccaccaagcg tttatcgaag atcgcgatat ccttcccggt cagtgtgctt cgaaatgatt
     5401 ctagcttctc tcgtagcagg ccgtggagtt gcgcgtcttc ggcgatccgg tcagggctat
     5461 ccgcgaccga gggcaagagt tcgagtcggg tcgtggcgcg gtcatcgccc tcggtgacag
     5521 gcgcatcgag cgaagcatcc tgacgggcca gccgtcgatc catctccatg acttcggatt
     5581 cttcgacgtt caggctcttg gcgatctcgg cgtgcgtggg ctcgatcccc atttgcgata
     5641 ggcgcgcctt ctctttgctc aagttgaaga acaatttgcg ctgcgcttgg gtggttccaa
     5701 tcttcaccat gcgccagttg ttcaggatga agcgcagcat gtacgcgcga atccaccacg
     5761 cggcgtagct ggagagcttt acgccccgat agggatcgta gcgcttgacc gcttgcatga
     5821 gcccgatgtt cccctcttgg atcaggtcca tcatgttgcg gtaagcacgc cgatattcgt
     5881 aggcgatctt gacgacgagt cgcaggttgg ccgtgaccaa gctcgccgcc gcattgacgt
     5941 cacccgtctt cttgtactcg acggccagag cgtgctcgcg atcgggcgta agtagcgggt
     6001 gacgctgcac ttcgcgcatg tacgcggcga gcgcgtcgac cttcgcgaga gcggtggaat
     6061 cgacctcggg ctccggttcg tgctcgacgg tttcgtcgga cagcacctct gccgtggctt
     6121 cgagcacatc cggatcgagg ttgcccgaat ccgcatcgag ttcggcgtcc acttcgattt
     6181 cggaatctgc gccgagctcc gattcggagc tctcgtcgtc cgcgtactcg tcggctgatt
     6241 cagagggctc acccgccgcg acgctcgcct tttcgacttc gcgttgtttg ccctgagcgc
     6301 gtggcttgct ggcggcgctc acctttcccg cagtgcgctg cgtacgttcc gatttggaag
     6361 ttttgctccg cgcaccagcg cgagagaccg tggcccgagc gggcctcggc tcgacgatcg
     6421 cctccccggc ttcgatcgcc ggggcgcttc ggtctgcgct gctcccatct gcggcatggc
     6481 ccgcctgagc accaccaggg ctccgggagc gtcccgcagg cttttcgggt ttgggtgagg
     6541 tcatactggc aggcctttgc gcatgttgtc cggcgtggag tttggggctg cgttcgcgag
     6601 gcggagtgag cgggacagac ttagcaggac aggcttagca gttcgcccaa ctaggcaagt
     6661 tatgcgcctg gagccggagt ctctggccga tagattacgc agcgaagggt gcgaccatcg
     6721 ccgacacgcg tcaagaatgc gcggcatcac ccgaaagggc agtgcgcgcg cagcactcgt
     6781 tccacccgat ggccgacccg cgagctttaa gtggagcgga cgcgagcgtt tttggctcga
     6841 ctaacgccgc tgcgggaacc gcactcctcg aacgcgcctg gctcgatacg tccatacgcg
     6901 tgaccttgga gggcaattgt aggttaggct gacgcctcga cgagggtaat atcccgcaca
     6961 ggccgggccc aacggatggc gaagcaaact gcacgaagct ggcgaaaggt cgccgcgcat
     7021 tttcgaagct cgaaacctgt gctcttcgaa gggtcgattg tttgagcggg acctagtgga
     7081 tcgggatgcg gccgagtgga ttcgggccgc tctcgatggt gcgccgtttc cggtcgggat
     7141 ttgcgccgaa ggccggctgg tctacgaaaa ctcgcgcttc agcgactgga ttctcgaacc
     7201 ggagctggct gcgcccaatg cgccggaatg tgcgccagaa cttccgccgg agcgctctta
     7261 caccacgcgg cggcaacagg tgcgcgagct ggtggccgac ttgtctcacg ttctgcctgg
     7321 acccagcgcg caacgttttg ctggaccggc tggggttggc gtgcctacgc ccttgagccc
     7381 gttcccctcc ggcggacccg gctccagccg attcgctcca gttgagtctc cgggacttgc
     7441 gagcgcagga gtcagtgttg gggtcgcgaa gagcgctact cgccaaacgc ggttcattag
     7501 cgggggcggc gg
//
LOCUS       DASTKU010000048         5269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1792163, whole genome shotgun sequence.
ACCESSION   DASTKU010000048 DASTKU010000000
VERSION     DASTKU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792163"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..609
                     /locus_tag="VFQ61_01555"
     CDS             94..609
                     /locus_tag="VFQ61_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953155.1"
                     /translation="MAAEWVTRRKLGLLGLAAITVGAGYALWGKSDERRVLEMLRDLA
                     RDLATTAEADEQERRRIAMAALSPRVAPRVELSVPEFGESEGIESLADLLAQSVGTAL
                     RFSIEQANIHVTHDQAMATLLGTVTAERPGQEIRDSRTVRVSLTRHGPRFRIVRIRVE
                     ERSRAQPEARP"
     gene            complement(664..1890)
                     /locus_tag="VFQ61_01560"
     CDS             complement(664..1890)
                     /locus_tag="VFQ61_01560"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9953156.1"
                     /translation="MGEAGFDDAERTSWCDATERAGGPAGNTREQRAQWIQRQAAELL
                     GLAGRLLGPHVGAREVVRDTFVAAFSSRESEAGASFAVITRNLLVELVLERLEERRFL
                     GFGARVLRPKFAALGPRLLPESSEGVRAELARFYEALLLLSPRTRVAFLLHEVEGLAT
                     AQIASSLRAAPLRVERWLQRADRHIARSCPAGAPSLQRLYSPLSSLELRHLTAQVESE
                     LGKMREQHPDLARSRRSRYVAGGAALGLIVPALWALGVPQSLTRDGRGRATTGVGALH
                     TAPSLTQADVQKLELPGGAQATLSATSQLKIEAAESGRVQLTLLSGQADFVIQHPRQQ
                     NFVVHAPGAALTAAAERFSVALERDRLNVHVQRGHVHVQPAGDDRTLNLGSGETWSGP
                     RERPRREPLEPPRAHP"
     gene            complement(2100..3104)
                     /locus_tag="VFQ61_01565"
     CDS             complement(2100..3104)
                     /locus_tag="VFQ61_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET9953157.1"
                     /translation="MTSAPLPHLASASVSPGAARAGFSRAAGWVLVYTVFVILFGAVV
                     RITGSGAGCGQHWPTCQGEIVHLPTRLATWIELSHRVTSGLSALVVFALTGFAFRRFP
                     SAHAVRRAAVIATAMMIVEALIGAVLVRLALVAGNTSAARAIVMPLHLISTSILTAAL
                     ALCWWWSRAAVPLPSSPITGASAVSAKASPRAARWVALAALGVLVVSAAGAVTALGDT
                     VYPVRAAPLAERLSHDQHASAHFLERLRVLHPLLAVLLAAALFVGVGKLLEAPVTEAT
                     RRLTRAVAWLTGLQVAAGGLNVMLSAPGWLQVLHLLIGTGLWLGLILLVSELYAPSAA
                     "
     gene            complement(3212..4075)
                     /locus_tag="VFQ61_01570"
     CDS             complement(3212..4075)
                     /locus_tag="VFQ61_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET9953158.1"
                     /translation="MRYTPGGPSEDIEDRRGQGGGGLGGGGLKLGLGGMLLVGLLSVV
                     FRRNLFADLSQVQGNAPSRATPGAPYQETPQEKQMVEFVSFVLDDVQNTWTREFQARG
                     QTYPRAKLVLFTDVVRSACGTAESATGPFYCPGDQKAYIDLSFYQDLKARFGAPGDFA
                     QAYVLAHEIGHHVQNVLGTEKRLRREQRANPADANALSVRMELQADCYAGMWGKSTQQ
                     RNLLEEGDVDEALNCATAIGDDRLQKQAGGRVSPETFTHGSSEQRKRWFKRGFESKRL
                     EDCDTFGTTTL"
     gene            complement(4275..4934)
                     /locus_tag="VFQ61_01575"
     CDS             complement(4275..4934)
                     /locus_tag="VFQ61_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953159.1"
                     /translation="MAALPLACGDDDDDVATGGKGGTSGATSGGKGGTTSTGGAATGG
                     KGGTTSTGGATTGGKGGTTSTGGAATGGKGGTTSTGGTVNQGGESGSAGEAGASGGSA
                     GEGGSGGGGGAGGASGGEGGMNGGGGAGGGTSDLTPCEQACAAAAKRMCANQAACEEN
                     TCTQLEAFVPDCPDERNAYLECLGRSPASDWTCPDDTPLYSSEECNGYLEAAILCQSM
                     K"
BASE COUNT          940 a         1736 c         1671 g          922 t
ORIGIN      
        1 gatcagctgg ccgagctcta tgcgttcgcg atcagcgagc cctatcgagc tctgcgccaa
       61 cgcctcggag tatccgtagc cggaggtctc tgattggccg cggagtgggt cacgcggcgg
      121 aagttgggac tgctcggcct agccgccatc acagtgggag cgggttacgc gctgtgggga
      181 aaatcggatg agcggcgcgt gctcgagatg ctgcgagatc tcgcgcgtga cctcgcgacc
      241 accgccgaag cggacgagca agagcggcga cgcattgcaa tggcggccct gagtccacgg
      301 gtggcgcctc gagtcgaatt gagcgttccc gagttcggag agagcgaagg catcgagtcg
      361 ttggcggact tgctggcgca gtcggtcggg acggcgttgc gcttcagcat cgagcaagcc
      421 aacatccacg tgacccacga tcaggccatg gcaaccctgc tgggcactgt cacggccgaa
      481 cggcccggtc aggaaatccg cgactccagg accgtgcgtg tatctctcac gcggcatggt
      541 ccgcgctttc ggattgtacg catccgggtc gaagagcgtt cccgcgcgca acccgaagcg
      601 cggccttgag cagcggtggc ccggcgcacg agtgggcgca cgttcgcagc gtgagccccc
      661 ttcctaaggg tgagcacgtg gcggttcgag cggctcgcgt cggggccgtt cgcgaggacc
      721 agaccatgtt tctccgctgc ctaaattcag cgttcggtcg tcaccggcgg gctgcacgtg
      781 cacgtggcct cgctgcacgt gtacgttcaa tcgatctcgc tcgagtgcga ccgagaagcg
      841 ctcggcggcc gcggtcaatg ccgcgccggg tgcgtgcacc acgaaatttt gctggcgcgg
      901 gtgttgaatg acgaaatcgg cctgccccga caaaagcgtg agctgtactc ttcccgattc
      961 cgcggcttcg atcttcagct gactagtcgc ggaaagggtg gcctgagcgc cacccggtag
     1021 ctccaacttc tggacgtcag cttgggttag cgaaggagcg gtgtgcaacg caccgacgcc
     1081 agtggtggcg cggccgcggc cgtcgcgcgt caacgattgc ggtacaccta gcgcccagag
     1141 cgcggggacg atcagcccaa gcgctgcccc tcccgccacg tacctcgacc tacgacttcg
     1201 agccaagtcg gggtgctgtt cccgcatctt cccgagctcg gactctacct gcgccgtgag
     1261 atgcctgagc tcgagcgatg acaacggcga gtacaatcgc tggagactgg gcgcgcccgc
     1321 cgggcaactc cgcgcgatgt gccgatcggc gcgttgcagc cagcgctcca ctcgaagcgg
     1381 cgccgcgcgc aacgagctcg cgatttgagc cgttgccagg ccttccactt cgtgaaggag
     1441 aaatgcaact cgagtgcggg gcgacagcag gagtagcgct tcgtagaagc gcgcgagctc
     1501 ggcgcgaacg ccttcgctgc tctccggtag tagtcgcgga cccaatgccg cgaatttcgg
     1561 gcggagaacg cgggcgccaa agcctaggaa tcgacgttcc tccaggcgct ctagaacgag
     1621 ctctaccagc aagttgcgag tgatcaccgc gaacgacgcg cccgcttcgc tctctcgact
     1681 cgaaaatgca gccacgaacg tatcgcgaac gacctcgcgc gcgccgacgt gtggtcccag
     1741 taagcgtccc gccaaaccaa gcaactccgc tgcctggcgt tgaatccact gcgcgcgctg
     1801 ctctcgtgta tttccagccg ggcctcctgc acgttccgtg gcgtcacacc aggaggtacg
     1861 ctcagcgtcg tcaaaaccag cctctcccat gcgcggagct acaaccagtt cccgatcgcg
     1921 agctggctga acgaccaagc gcaaagttgg gtgagttttg ctcacttcac cgcggtactt
     1981 tttcgaggcg ttcgaggttc aaaaaagaac cgtcacccct caaaaaaatg cggcgtcgtt
     2041 ggagcttgcg cctggaagtc tcgccatggg cgcctgctgg cgagagcaaa caccgtgttt
     2101 caggcggcgc tcggggcgta aagctcgcta accagcagga tcaggcccaa ccaaaggccg
     2161 gttccgatca atagatgcaa cacctgcagc caacccggtg ccgagagcat cacattcagg
     2221 ccgccggccg cgacttgaag cccggtcaac caagcgaccg cgcgcgtcag acgccgggtg
     2281 gcctccgtga ccggcgcttc caaaagcttg cccacgccca cgaagagagc cgctgcaagt
     2341 agaaccgcga gcagcgggtg cagcactcgg agccgctcca agaagtgtgc ggaggcgtgc
     2401 tggtcatgag acaggcgttc ggcgagcggc gccgctcgaa cgggataaac ggtatcaccc
     2461 agcgcggtca cggcgcctgc ggccgaaact acgagtaccc cgagcgcagc cagcgctacc
     2521 cagcgcgcag cgcgcggtga cgctttggca gaaactgctg aagcgcccgt gatgggcgac
     2581 gacggcaagg gcaccgcggc gcggctccac caccagcaga gggcgagggc cgcggtcaag
     2641 atcgacgtgc tgatcaggtg caggggcatc acgatcgcgc gtgcggccga agtgttgccc
     2701 gcgaccagag ccaggcgcac gagcacggca ccgatcagcg cctcgacgat catcatcgcg
     2761 gtggcgatca cggccgcgcg gcgcacggcg tgggcgctcg gaaatcttcg gaacgcaaag
     2821 cccgtcagag cgaacacgac caacgcgctg aggcccgagg tgacccggtg gctgagctcg
     2881 atccaagtgg ccagacgagt cggcaagtgc acgatctcgc cctggcatgt tggccaatgc
     2941 tgcccgcaac ccgcgcccga tcccgtgata cgaaccacgg ctccgaacag aatcacgaac
     3001 accgtataaa cgagcaccca gcccgcagcg cgcgaaaagc cggcgcgggc agcgcccggt
     3061 gagacgctag cggaggccag gtggggtagg ggagcggagg tcactagacg gcatcgggca
     3121 tgccacggcc gcacgggatt gtcgatgcgc cgaccgttcg cactcgcaaa ccgcagctgc
     3181 gccgggccaa ttgtcgtgtc ttcgcgcgcg gctagagcgt ggtggtgccg aacgtatcgc
     3241 agtcttccag acgcttgctt tcgaagccgc gcttgaacca gcgcttgcgt tgctccgagc
     3301 tgccatgcgt gaaggtttct ggggagacgc ggccaccggc ctgcttctgc aagcgatcgt
     3361 cgccgatcgc ggtcgcgcag ttgagggcct cgtccacgtc tccttcttcg agcagattgc
     3421 gttgctgcgt cgactttccc cacatacccg cgtagcagtc cgcttggagc tccatccgta
     3481 cgctgagcgc gttggcgtcg gctgggtttg cacgttgctc gcgccgcaaa cgcttctcgg
     3541 tcccgagcac gttctgtacg tgatgcccga tttcgtgcgc gagcacgtag gcctgcgcga
     3601 agtcacccgg cgcgccgaag cgcgccttga gatcctggta gaaggaaagg tcgatatagg
     3661 ccttctgatc gccggggcaa tagaagggcc cggttgcgga ctcggccgtg ccgcaagcgg
     3721 agcgcaccac gtccgtgaac aatacgagct tcgcgcgggg gtacgtttgc ccgcgcgctt
     3781 gaaactcgcg cgtccaagta ttttggacgt cgtcgagcac gaagctcacg aactcgacca
     3841 tctgcttctc ttgcggcgtc tcttggtaag gagcaccggg agtggcgcgg ctcggcgcat
     3901 ttccctgcac ttgcgacaaa tcggcgaaca ggtttcgccg aaagaccacg ctgagcaggc
     3961 ccacgagcaa catgccaccg aggcctaact tcagaccccc tccgccgagg cctccgccac
     4021 cttgaccgcg ccgatcttcg atgtcctcgc tagggccgcc gggagtgtat cgcatgcgac
     4081 gacatcgtag cctggtcgcc gaccgtactc aacgcgggcg aggggagtcg gccagtttgc
     4141 gatggatagc gccaaagcgc gccgcgctga tctgccgcat gcgatttccc cagcgcggtt
     4201 tcggacgcaa acaatccaga gacgagggcc acattggccc tcgtcagcct gggatcacgc
     4261 aaactggaaa ctcactactt catggattgg cacaggattg cggcttccaa atagccgttg
     4321 cattcctcgc tgctgtatag cggcgtgtca tcggggcagg tccaatcgct agcggggctt
     4381 cggccgagac attccaagta agcgttcctc tcatcgggac agtcgggaac gaaagcctcg
     4441 agctgagtac acgtattttc ctcgcaggcc gcttggttcg cgcacattcg cttcgccgcg
     4501 gccgcgcagg cttgttcgca tggggttaaa tcgctcgttc ctccgcccgc gccgccgccg
     4561 ccattcattc cgccctctcc gccggaagca ccgcctgcgc cgccaccgcc tccggagcca
     4621 ccttcgcctg cgctgccgcc ggacgcgcca gcctcacccg cagagcccga ctcgcctccc
     4681 tgattaaccg tcccgccggt gctggtggtt ccgccctttc cgccggtggc tgcgccgccg
     4741 gtgctggtcg ttccaccctt tccgccggtg gtcgcaccgc cggtgctggt ggttccgccc
     4801 tttccgccgg tggctgcgcc gccggtgctg gtggttccac cttttccacc tgaggtcgcg
     4861 ccactggttc cgcctttgcc accagttgcg acgtcatcgt cgtcgtctcc acaggcgagg
     4921 ggaagtgcgg ccaatgcgat tacaagcgca agcgttgctg tgtttctcat gatgctcctc
     4981 gttgttcctc ggataagaat gcccacggta ggccccgtga tgcctagttt tcggacgcgg
     5041 ttagagctct cgggctatcg gcacgaccag tgtgcctcaa agcgccattc tcacccaggg
     5101 gacgccaaac gtcccctgta gcagccttgc tggagtcaga gcgccgcgca tagccgcgcc
     5161 ccaaagcagt gtcgcttggt gttcgtttcc agctctccat gtcctcttga tcgaaatcga
     5221 tcgcgcgaca ccttcaaggc ccggtgatgg cttacgtgta ggtcagggc
//
LOCUS       DASTKU010000049        14034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1124033, whole genome shotgun sequence.
ACCESSION   DASTKU010000049 DASTKU010000000
VERSION     DASTKU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14034
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124033"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(562..963)
                     /locus_tag="VFQ61_01580"
     CDS             complement(562..963)
                     /locus_tag="VFQ61_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953160.1"
                     /translation="MEPVVLFEEESLILWYHPDENIVHHQLRKFPDSGTFREMLTRGA
                     ECLEQYKSKKWLSDDTGNVVVRDSDIEWGNSVWVPRVLRAGFKFWAIVMPVSAVGQLS
                     MQRIAAAHRKLGVNAQTFSDLSSAFSWLKAM"
     gene            1621..2826
                     /locus_tag="VFQ61_01585"
     CDS             1621..2826
                     /locus_tag="VFQ61_01585"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET9953161.1"
                     /translation="MAERYQRNQRPNDMQTQGARRGSEEHGPRWQEEHRWQEDQRSHG
                     RENYRSSGRDNRGGRDPRSPSRDASSRDFGESLHHYAHDQEAAMHDRERWGDADLYGG
                     PVWDQEHYQDPNEHNEYHSRGYGSANYAQPRANPRSREGGYGQGGFGSAQYPGRGYSG
                     QQGYSGGQAGSRQSGFGQSNNDYGNRYDQGGYGRPGYAQGYGQTGFGHSGYSPDAQNY
                     RPGSGSPGFNSPRYGAPLTSDNRGSNWGNTSNQRSESFYGRGPKGYTRSDERIREDIC
                     DRLSDDDELDASDISVSVKNGEVTLEGEVADRASKHRAEDIADAVSGVTDVHNRIRAR
                     KGLMRELEDKLTGREETPHGHAGSGTKNTPSTHGSTGTETTASTSSTGTTTGSSAGTT
                     SPIRNGVSG"
     gene            3321..3983
                     /locus_tag="VFQ61_01590"
     CDS             3321..3983
                     /locus_tag="VFQ61_01590"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HET9953162.1"
                     /translation="MLTFRVGLACALSLLTVTLSSVTLAEDAIVRIAPEQFQIVKRDS
                     GPVDYYSVRKASTPPHIRAEYRPPLKTVVLGYQVPDSLRQAGKLSWSWRALELPKGGD
                     ECLRGKQDSAAVVYVSWRRGLRWYTLKYVYSAVGRKGAICDRKRNPFLAQDTVILRTG
                     GPLETWQQETLDLKAEFRQHFEPDDPDAPVPDLLGIGIMTDGDQTQSRSSADYAQFVL
                     NK"
     gene            complement(4044..4829)
                     /locus_tag="VFQ61_01595"
     CDS             complement(4044..4829)
                     /locus_tag="VFQ61_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953163.1"
                     /translation="MSEAGEGSANVNVSSVGLLQTEFTRTDSVLTPTDSVLTPTDSVF
                     TPTDSARSYPLRVGRRPLAIAALGLLFGLAAPAGIAPAHADERRPEKAERYRRPVPSQ
                     SIEAGCARLGVNASLETVRKVVNDFDRYASFVKRYKDGRLQLQVTAKLLGRSGEKKDV
                     YLEVPILRGTSKVWGMLRFDPVKVVGDEEILEGHLLKGNVERLDARWRLRRIEAGRTL
                     VALELLIVPKVPVPRDVVTGELEFVSDVSVSGVRNEAERIDAP"
     gene            5523..7031
                     /locus_tag="VFQ61_01600"
     CDS             5523..7031
                     /locus_tag="VFQ61_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953164.1"
                     /translation="MIRNASLGSSALVRAARVSLLCAVALGAIVACGGSSKGDKKSPA
                     AGGDSDMSAGSGGTQNSETGGGGTRPGTKTDAGSGARSGGSTGTSGGAVGSGAVGNST
                     ATGGRGSGLGGDSDGGVESSGGDSGTTPNGSCAEAAQLECEYQKKCDPVRFALEWDGN
                     LDDFCVPHTTAACEAGVATRFDGLNQACSDWVQASCANYLSFYYPILDSNGDRVNWNS
                     ACDAKPVQGDRKLNQACNYDAQCAAGLFCRFDADMPDAKADADRTCGQCAAKLAYPQS
                     QFPLRTGLFSGDLEDGAYCNRDTQCADGYACVLPYDLNDGEPKVLELVHESRHCMLIT
                     KQDSDDLCVRGSNLECDRSSNLVCPEGAPTDDPETSPHCTTAPTATVKKYLEAGAACK
                     GTDECNPQKHLTCVPDAEDVKRCTLPRVAAIGEPCGDNVSCSQFARCNKNNICERRRE
                     NTQACDDSTPAVAATYCDPAVFANEQALVCRTATGEITPRCQPPLPGDNVCQ"
     gene            complement(7285..7629)
                     /locus_tag="VFQ61_01605"
     CDS             complement(7285..7629)
                     /locus_tag="VFQ61_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953165.1"
                     /translation="MRNILVLSGCVLALACASRETPPATDASSSSTVDQATESPNNGP
                     DFTPTSPDGAFGAKADGTAADATSPNPSQKPTDTQGAGMPPSGTGTSGTGTSGAGTGG
                     PADTSPAPNPRR"
     gene            7822..8343
                     /locus_tag="VFQ61_01610"
     CDS             7822..8343
                     /locus_tag="VFQ61_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953166.1"
                     /translation="MLLRPQAAFPLARELQSVSGAALGDVFSFISGLYFRGKVAYAGK
                     YGDLPGGNGGGLVITPGEGLKFLQERVTVERLLAWAKVNVDESNPAFTEPLIGHCYEL
                     TRVLGESARYVLLGSIATRKYLAPLSQGLGSERLWFPESFLGRGDMSRGALMLRAARS
                     GDELTYRQVSALR"
     gene            complement(8373..9278)
                     /locus_tag="VFQ61_01615"
     CDS             complement(8373..9278)
                     /locus_tag="VFQ61_01615"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9953167.1"
                     /translation="MSLSALPLPPQSSAGLPPCASEVVLRRVTIQFGAGATAVHAVEN
                     LSLTVAPGEFVSIVGPSGCGKSTLVGAVAGFTPLTQGEITVDERPVRGPGADRGVVFQ
                     QHALFPWKSVRKNVEFGLKMRGIGRLERARIAREMLAQVGLGEFAEHYPHQLSGGMQQ
                     RVNLARVLVNQPRVILMDEPFGSLDAQTRLQMQQLLLGLWHERGMTVIFITHDIDEAV
                     FLSDRVIVLSRRPGAVILDARVELPRPRSYDVLTSPEFGALKRRTLELLLGQTHDSAE
                     HRLAQHGLAEGDSAEHLPCLEPSRK"
     gene            complement(9275..10135)
                     /locus_tag="VFQ61_01620"
     CDS             complement(9275..10135)
                     /locus_tag="VFQ61_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9953168.1"
                     /translation="MANSAIGIMWDRAARGRPKALRREWRRLVPWLVSVSSLVVWLAL
                     WQVASSHKWNFFFRFENVPAPTEVAAAMGDLFLAPKFVDHVLHSLRRILFGFAIASSL
                     AVGLGLLIGRFRLAAYSLCPALEVLRPIPAVAWIPLAILLFASAEHSMVFITFIGAFF
                     PVLLNTVHGVEALDRRLIHASMTLGARAFDIFWDVILPGALPSIVTGLSIGMGTSWFS
                     LVTAEMISGQFGIGYYTWEAYTLQNYPHIVLGMLTIGTLGMASSLVIKWSGERLTPWL
                     VRETKSLGTA"
     gene            complement(10198..11661)
                     /locus_tag="VFQ61_01625"
     CDS             complement(10198..11661)
                     /locus_tag="VFQ61_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9953169.1"
                     /translation="MNRAFHALIFLCGTLLLPACRAKDVAASESSTAKNKPGEGAEAR
                     IRIAVGTQDTTINCAAGGPLIRELHLLERYLPHDGRYAGVQYDIRWENQPTGAQLNSK
                     YLANQLDIVQMADFPSVIGASALKAAPGGVESFYIASLAGGIRGAGNAILVPADSTVQ
                     SLSELRHKKISVPFGSTAHAMLIRAVQDLGWDPKSDVEIVTQTPEVAGSALRAHQIDA
                     HANFVPFGELFPFRGFAKKIYDGSSTGVTTTHGVQVRSDFATKYPELVVAYLKVTLEA
                     DRLFREDPEGLSERLATWTGVDAEVYYAFHGPQGIQTRDYSLKPEYIAAIRRAQDSLK
                     VLKKVDKEIDVDRYVNDKFIRQAAKEFGYDYDARLKDYTALPFAGEAIDTHAKVEEPS
                     LAGQLWVKGEPKVRLYSTIEATFSALRRLRAEGKSLRVAFVHDRLSGNKLFADKVWYV
                     NQHGRLAAFLSKEAAVQWAAQHGGAVIGYEAVQRQFQ"
     gene            complement(11680..12699)
                     /locus_tag="VFQ61_01630"
     CDS             complement(11680..12699)
                     /locus_tag="VFQ61_01630"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9953170.1"
                     /translation="MTKLTEPTRTTSLTESTRTKELSEILSELASPDPERRQLALLRL
                     EGSLDASVVDALIARTRDPDSTVRALAVQLLEEVGEQRSARPLIELLADPNLDVRERA
                     GEALRSLRGPSVLEALLRAIEHPLASVRAGVILALRDLREPLAASALVLRLADSDPEV
                     RREAVLALGFLRSQAESPSLIGALSDDEPRVRRAAIGALDYASSPAVVSALGRALNDS
                     DWRVRADAARALGQRRACELAGPLIARTQDTQWQVQREALLALAACAAEDAAPEAVRL
                     LESALADVRRAAIELCSAIRLRAALPRLRSLAHDEDVEVRKAAKRAVSVLQALADTQP
                     LNGNN"
     gene            complement(12739..13038)
                     /locus_tag="VFQ61_01635"
     CDS             complement(12739..13038)
                     /locus_tag="VFQ61_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET9953171.1"
                     /translation="MSELGDRESVKAAHARALAQQPTTLSQTSLRVSVPVVVDEQKCL
                     KNCRVCIDSCPVDCLAISPVSGKAHMRYDECWYCLACEIDCPKDAITVQIPYLLR"
     gene            complement(13084..>14034)
                     /locus_tag="VFQ61_01640"
     CDS             complement(13084..>14034)
                     /locus_tag="VFQ61_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HET9953172.1"
                     /translation="TVNANGHRFIESDYWSGQMMLEFYRELQSSNGPVYLELTHLAPE
                     TISEIEGVLHRTERPSRGRFHHGRGHSYSSHRVEMHISEIGLCSGHSASGVFVNERGE
                     TTVDGLYSAGDMACVPHNYLLGALTYGQICAENAVRFLASAGIGSEPSEDAVAQERAR
                     VLAPLSRPHGLPHHQYEYKVRRLVNDYLQPPKTAKRMESGLAQFMRAREELDELGAAN
                     PHELMRVMECHFIRDCAEMAARASLFRTESRWGLYHYRLDFPDLNDRDWFVHVNLRKS
                     AAGEMEAFRRDVEPYVVSLSSDELRAYHKLRVNELSLASA"
BASE COUNT         2748 a         4462 c         4213 g         2611 t
ORIGIN      
        1 gctcttcgct cgactgacgg ctcctcgctc gactgacggc tcctcgctcg actgacggcg
       61 ctctcctcaa ataatggagc ttcgctctgc ttgatggcgc tcccctcaaa taatggagct
      121 ccgctctgct gatggcgctc ccctcaaata atggagcttc gctcaactaa tggagcttcg
      181 ctctgccgat ggagcttcgc tctgccgatg gagctccgct cgactgatgg agggccgccg
      241 gggggcgggc gctgaggctg gctccgactc cgacctacgg ctctttacag caactcccgt
      301 ggccaagcat tctgcctaat agagctcctt ggccagaacc gcgcacgact ctcgctactt
      361 atcggcggcc cgcatttttt ccactgaaat ccgcggcgct actgagccgg cctttcccgc
      421 gcacgacgcg ctaagtgggc aaagccggag agagatggct tcaggttggc ggtcggtgtg
      481 cgtcgcgata gcaggcggaa ggttaggact gagcaggctt ttgaactgca cgctttggcg
      541 cgacatcgag gctgaaaggg cttacatggc tttgagccag gaaaaggcgc tcgagagatc
      601 actgaaggtt tgcgcgttga cgcccagctt ccgatgcgcc gcggcgatac gctgcatact
      661 cagctgaccc acggccgaga cgggcatcac gattgcccag aacttgaacc cggccctcaa
      721 gacccgaggc acccagacgc tgtttcccca ctcgatgtcc gaatctcgaa ccacgacatt
      781 accggtgtcg tccgagagcc atttcttact cttgtattgc tcgaggcact cggcacctcg
      841 agtcagcatc tcgcgaaatg tgccggagtc tggaaatttc cgcaactggt ggtgaacgat
      901 gttctcgtcc gggtggtacc agagaatcag cgactcttct tcgaacagaa ccacaggctc
      961 catgctgacc tgaccaagtc gtatcattag cccccccgtt tggcgatgga ttgccggcgg
     1021 tcgggttcga gtgagtgtga gttcgattcg cctaagtacc cacgcccgac ctccacaact
     1081 aacaacaaca cgggtcattg accgcacaaa atccctgatc cttcacgcga aggccccacg
     1141 aaactcttgt cgcagctcag taccaacatc tctgacgagc aatcgtcttt gcccagggtc
     1201 ctttcactgg gcgtgccggg ccatctcggc ctatccggcc gaaggtgacc gaaggctgat
     1261 cggaactgct cttcaactct tcggtgtcgc tgagagcaca ctggatttgc actgccgggc
     1321 aggagatgta gcgggtttgc tctccgccga gtcgcgtcga gtcaagggtc cagagcgccc
     1381 ttcgcgctgc tcacggttcc gtgcgcatga accgggacac ggagccacgg tgcattactc
     1441 agcacgcgga tgagcgtccg ccttcctact gcctctagag ccgtgtccgg cacgcacacg
     1501 catacagctc ggcacatggt ttgcagccga ttgctctcgt gtccgcaacg tttcggacat
     1561 tcagaagcct cgtcgccaca tctgcgacga gtgcgaattc tccacaagga gaggattgtc
     1621 atggccgaaa gataccaacg caaccagagg ccgaacgata tgcagaccca gggcgctcga
     1681 cgcggctccg aagagcacgg ccctcgatgg caagaagagc accgttggca agaggaccaa
     1741 cgaagccacg gtcgcgaaaa ctaccgctcg agcgggcggg acaatcgggg cggccgagac
     1801 cctcgttccc ctagccgcga tgcctcgagc cgtgactttg gcgaatccct tcaccactac
     1861 gcccacgatc aagaggcagc aatgcacgac cgagagcgct ggggcgacgc agatctctat
     1921 gggggaccgg tttgggatca agaacattac caggatccca acgaacacaa tgagtatcac
     1981 tcgcgcggtt acggctccgc caactacgca caaccccgcg cgaacccgcg gagccgcgag
     2041 ggtggatatg gccaaggcgg atttggttct gcacagtacc cgggacgcgg gtactcgggg
     2101 cagcagggct attcaggagg gcaggctggc tcgcgtcaga gtgggttcgg tcagagcaac
     2161 aacgactatg gtaaccgcta tgaccaaggc ggttatggtc gccccggtta cgctcaaggc
     2221 tacgggcaga ctggctttgg acatagcggc tactcgcccg acgcccagaa ttacaggccc
     2281 ggctccggta gcccgggatt caatagccca cgttacggag ccccactcac gagcgacaat
     2341 cgtggttcca attggggcaa tacttcgaac caacgctcgg agagcttcta cggccgcggc
     2401 ccgaagggct acacgagatc ggacgagcgc attcgcgaag acatttgtga ccgattgagt
     2461 gacgatgacg agctcgacgc gagtgacatc tctgtgagcg tcaagaacgg tgaagtgaca
     2521 ctcgaaggcg aggtcgcaga tcgtgcgtcc aaacatcgcg ccgaagatat tgcagacgcg
     2581 gtcagcggcg ttactgacgt gcacaatcgg attcgcgcgc gcaaaggtct catgcgcgaa
     2641 ctcgaggaca agctaaccgg gcgtgaggag acccctcacg ggcacgccgg ctcggggacc
     2701 aaaaacaccc cgagcacgca cggcagcacg ggcaccgaaa cgaccgcttc gacgagcagt
     2761 accggcacca ccacgggcag ctctgctggt acgaccagcc ccatccgaaa tggcgtttcc
     2821 ggttaagttc gtgccggtaa tgctgcaatg cctctcgggg tgagccacat catcaaggct
     2881 gactgctcgc catcccgaca tacaggctcg gtcagcagtc ggccttagaa catgtgggag
     2941 cgccgcgacg gcggccgaga gcgaagcgga atccgcgccg catttggatg acggcgcgcg
     3001 gaggcccagg ctgggagagg ttcttaggac gaaagcttgt ggggggtatc tggccgcccg
     3061 agtaacgagc gcgccgcgcg tccttttccg gaagaagcgc agccggcgcg ctcggcgaca
     3121 atcgtcagct ccgcagcgaa gctggtcgcg aaccctcacc ttagagccgc ccctcgcaac
     3181 accgcgcaga ggcctccgcg taacatcttc tgcagaggaa tcggtgagca aagcgcgtgc
     3241 tccggtccac gcacgagacg gtttgccggc agtgatccgg cattgacgtt tggcgttttg
     3301 gcgccatcac acaaggcgcg atgcttacct ttcgtgtcgg tctggcctgc gcgctcagcc
     3361 tgcttaccgt caccctgagt tccgtgacgc ttgccgaaga cgcaatcgtc cgaattgctc
     3421 ccgagcagtt tcaaatcgtc aagcgggatt cggggcccgt cgactactac tccgttcgga
     3481 aggcttcgac gcccccgcat atccgtgccg aataccgccc acccttgaaa acagtagtgc
     3541 tcggctatca ggttccggac agcttgcgcc aagcgggcaa gctcagctgg tcgtggcgcg
     3601 ctctggagct accgaaaggg ggcgacgagt gcctgagagg caagcaagac tcggccgccg
     3661 tcgtttacgt gagctggcga cgcgggttgc gctggtacac gttgaagtac gtgtacagcg
     3721 cggtgggtcg caaaggggca atctgtgatc gcaagcgcaa cccgttcctc gcgcaagaca
     3781 ctgtaattct gcgaacgggc gggccgctcg agacctggca acaggagaca ctggacttga
     3841 aggctgagtt tcgccagcac ttcgagcccg atgatccgga cgctcccgtc ccggatctgc
     3901 tcggcatcgg catcatgacc gacggtgatc agacccagag ccgtagcagc gccgattacg
     3961 cccagttcgt gctcaacaag tgagcggcac gtcgagccgc gaggcggccg ggcccgcccc
     4021 cggagcgcgc tcgtgcatcg aactcacgga gcgtcgatgc gttcggcttc gttccgcacg
     4081 ccggataccg agacatccga gacgaactcg agctcacccg tcacgacgtc ccgcggcacg
     4141 ggcacctttg gaacaatcag caactccagg gcgaccagag ttcgccctgc ctcgatgcgc
     4201 cgcagacgcc agcgcgcgtc gagccgttcg acgttgccct tcaggagatg cccttccaag
     4261 atctcctcgt cccccacgac cttcacgggg tcgaaccgca gcatgcccca gaccttggat
     4321 gtaccgcgta agatcggcac ctcgaggtac acatcctttt tttcgccgct gcgaccgagc
     4381 agcttggccg tcacctgcag ctgcaaacgg ccatccttgt agcgcttcac gaacgaggcg
     4441 tagcggtcga aatcattgac taccttgcga accgtttcca gcgaggcgtt gaccccgagc
     4501 cgcgcgcagc ccgcctcgat gctttgactg ggcaccggcc ttctgtagcg ctccgccttt
     4561 tccggccgcc tctcatcggc gtgcgcggga gcgatccccg cgggagccgc gagcccgaag
     4621 agcaacccca gggccgcgat cgcaagggga cgccgaccca cccgaagcgg gtacgaccgc
     4681 gctgaatcgg ttggcgtgaa cactgagtcg gttggcgtca acactgaatc cgttggcgtc
     4741 aacactgagt ccgttcgcgt gaactcggtc tgcagcagcc ctacgctcga tacattcacg
     4801 tttgctgacc cttcaccggc ttcggacaat gcaatccctc gtctcgcaga tggagatagc
     4861 ttcatccacg ggccagcgca aattcgccca ttctgctcgg caaaggcggc tactcagccc
     4921 acctcgggcg cgcatgtgac ccccacccga catcggcgtc gaaagctctc cgcgcgccca
     4981 ttgatgccga gctcacgccc cgcgcttaca tggctgttcc atggtttcga gcaagtacgc
     5041 gcctacccgg gactcggccg ctcttcgtcg cagactgctg gcgagtgcca ccagtcgcgg
     5101 ctgcctcttc gtgaaacggg ttgccgtttg cccttccaag aggcttgcac tttagttcct
     5161 gaggcaccgc tgatttcgtg ctgacgagcg ctactcccga tgaaagacgc atgctgattc
     5221 ggaaatcgcc aactcaggac ccttcgacta gcgaaggtac tgactcccgc gatcctcacg
     5281 ctccgatccc cagtttcgaa ccgcagcttc gagacaacgt tcccaacatg agtgaccgcg
     5341 cgagatcgct tcgggagtcg atgagctggc ggtccgcacc gtccctctgc aaccggtccc
     5401 agtaaaccag ccgaccgcgc gcgagtaaaa aaatccacca aacgcgctac ttgggcttgt
     5461 gttcgagggc tccttcgcgg atactcgccg cgaaattggg ttcgttgtgg aggaggtcct
     5521 tagtgatccg taatgcgtct ttgggaagtt cggcattggt gcgcgccgcg cgtgtcagtt
     5581 tgctgtgtgc ggtcgcgctc ggagccatcg tagcttgcgg tggctcttcg aagggcgaca
     5641 agaagagccc ggccgcgggt ggggacagtg acatgagcgc cggcagtggc ggcactcaaa
     5701 actcagaaac gggcggcggt ggaactcgcc ctggaacgaa gacggatgct ggctccggcg
     5761 cacgcagtgg cgggagcacc ggtacgtccg gcggagccgt cggcagcggc gcggtcggca
     5821 acagcaccgc aaccggcggt cgcggtagcg ggctcggcgg tgactcggac ggcggagtcg
     5881 agtcttccgg cggtgactcg ggaacgacgc cgaatggcag ctgtgccgaa gccgctcagc
     5941 tcgagtgtga ataccaaaag aagtgcgatc ccgtgcgctt cgcgctggag tgggacggca
     6001 acttggatga cttctgcgtg cctcacacta ccgcggcatg cgaagccggc gtcgccaccc
     6061 ggttcgacgg cttgaatcag gcctgttcgg attgggtcca ggctagctgc gccaactacc
     6121 tcagcttcta ttatccaatt ttggactcga acggcgatcg ggtgaattgg aactcggcgt
     6181 gtgacgccaa accggttcaa ggcgaccgca agctcaacca agcgtgcaac tacgacgctc
     6241 agtgcgccgc cggtctgttc tgtcgcttcg atgccgacat gcccgacgcc aaagcagatg
     6301 cggatcgcac ctgcggtcaa tgcgcggcca agctcgctta cccgcagagc caattcccct
     6361 tacgcacggg gctattctcg ggcgatctcg aagacggagc gtattgcaac cgcgacacgc
     6421 agtgcgccga cggatatgcc tgtgttctgc cgtacgactt gaacgatgga gaaccgaagg
     6481 tgctggaact ggtgcatgag tcgcggcact gcatgctcat caccaagcag gattcggacg
     6541 atctctgcgt gcgcggctcc aacctggagt gcgaccgttc gagcaattta gtgtgccccg
     6601 aaggtgcgcc gacggatgat ccggagactt ctccgcattg cacgacggca cccacggcga
     6661 cggtcaagaa gtacctggag gccggcgccg cgtgtaaagg cacggacgag tgcaaccctc
     6721 aaaaacatct cacgtgcgtg ccggacgccg aagacgtgaa gcgctgtacg ttgccgaggg
     6781 tcgcagcgat cggcgagcct tgcggagata acgtcagttg cagccagttt gcccgctgca
     6841 acaagaacaa catttgcgaa cggcgccgcg agaacacgca agcctgcgac gacagcacgc
     6901 ccgcggtcgc cgctacctat tgcgatccgg ccgtgttcgc aaacgagcaa gcactggtgt
     6961 gtcgtactgc caccggcgaa atcacgccgc ggtgccaacc tccgctgccc ggtgacaatg
     7021 tttgccaatg atcgcattct cgcgcctcgt cccctgagtg cttctgtcat ccggctcgca
     7081 aacgtcatgc ttgcagccgc ggattgacac cccagccact cactaggtag cgcggcttcc
     7141 tagtaacggc cgagtccgca cagaagcagc gacgaacgcc tcggatggtt tcactgtccg
     7201 aggcgttctc gggttttgtg ccctagcggg cgcaggagga aaccctccat ccccctccag
     7261 caacacaatg gtgttagcgc gaaatcaacg acgcggattg ggggccgggc tcgtgtctgc
     7321 gggcccgccc gtgcccgccc cactcgtgcc cgtcccgctc gtgccggttc cactcggcgg
     7381 cattcccgca ccctgcgtgt ccgtcggttt ttgggaaggg tttgggctcg tcgcgtccgc
     7441 cgcggttccg tcggctttgg cgccgaacgc tccatcgggg gaagtggggg tgaaatcagg
     7501 accgttgttg ggagactctg tggcttgatc caccgtggaa gaggatgagg catccgtcgc
     7561 agggggggtc tcgcgcgacg cacaggccaa agccagcaca cagccagata gaaccaggat
     7621 atttctcatg gtcgttcctc gtgccgcgcg attccacgct cctcgcgatt ggcgcggcaa
     7681 aggtcatcca gcaagctttg ggccggcgcg ctgcctcgcg tgcatcgagg caagaccgcc
     7741 ctagactgaa gcattcttcc gactgcttca cgcaccatct tcctgctatc tcccgccaat
     7801 ctcgggggtc agcgcgcaaa gatgctcctg cggccacaag ctgctttccc cctcgcgcgc
     7861 gagctacaga gtgtgtcggg agccgccctg ggggacgtgt tcagcttcat aagcggcctc
     7921 tatttccgcg gtaaggtcgc gtatgccggg aaatatggtg acctgccggg tggcaatggt
     7981 ggcggactcg tcataacacc cggagagggc ctcaagttct tgcaggagcg ggtcacagtc
     8041 gagcgcctgc tggcgtgggc gaaagtgaac gtggacgaga gcaatcccgc cttcaccgag
     8101 cctctgattg gtcattgtta cgaattgacg cgggtcttgg gggaaagcgc caggtacgtg
     8161 ctgctcggta gcatcgcgac ccgtaaatac ctggctccac tcagtcaggg gctgggcagc
     8221 gaaagattgt ggttcccgga gagtttccta ggtcgcggcg acatgagccg aggcgcgctc
     8281 atgctcagag ctgcccgaag tggagacgag ctcacctatc gacaggtatc cgcgctgcgc
     8341 tgaactcgcc atctgctgaa gagtgcacca actcacttcc gactcggctc caggcatggg
     8401 aggtgttcgg cggaatcgcc ctccgctaag ccgtgctgcg ccaacctgtg ctcagccgag
     8461 tcgtgcgttt gtccaaggag gagctcgaga gtgcggcgtt tgagcgcacc aaactcgggg
     8521 ctggtgagca cgtcgtagct gcgaggccga ggcaactcca ctcgcgcgtc cagaatcacg
     8581 gcgcccgggc gtcgcgacaa cacaatcacg cgatcgctca agaacacagc ttcgtcgatg
     8641 tcgtgcgtga tgaagatcac cgtcataccc cgctcgtgcc aaagtccgag cagcagctgc
     8701 tgcatttgaa ggcgcgtttg ggcgtccagc gatccgaacg gctcgtccat caggatcaca
     8761 cggggctgat tgaccaagac gcgcgccaag ttcacgcgct gctgcatgcc accggacagc
     8821 tgatgcggat agtgctccgc gaactccccc aaacccacct gggcaagcat ctcgcgcgcg
     8881 atgcgtgccc gctcgagtcg ccctatcccg cgcatcttga gcccgaactc gacgttcttt
     8941 cgcacgctct tccaggggaa cagcgcgtgt tgctggaaca ccaccccgcg atcggcgccc
     9001 gggccccgga cgggccgctc gtcgaccgtg atctcgccct gggtcagagg ggtaaatcca
     9061 gcgactgcgc ccacgagcgt cgatttcccg caacccgacg gcccgacgat gctgacgaac
     9121 tcaccgggcg caaccgtgag actcagattc tccaccgcgt gaacagccgt tgcacccgcg
     9181 ccgaactgga tcgtcacccg gcgcagaacc acttcggagg cgcacggcgg caggcccgcg
     9241 ctgctttgcg gaggcaaagg aagcgccgac agactcatgc ggtccccaaa gatttggtct
     9301 cacgcacgag ccagggcgtg agccgttcgc cagaccattt gatgactagg ctgctcgcca
     9361 tcccgagagt gccaatcgtc aacatcccga gtacgatgtg cggatagttc tgcagcgtgt
     9421 acgcttccca cgtgtagtag ccgatcccga attgaccgga gatcatctcc gcggtcacca
     9481 aggagaacca tgatgtaccc attccgatgc tgagcccagt gacaatcgac ggcagagcgc
     9541 cgggcaggat cacgtcccag aatatgtcga aagcacgggc gccgagcgtc atcgacgcgt
     9601 gaatcaaccg gcgatcgagc gcctccacgc catgcaccgt attgagcagt accggaaaga
     9661 aggcaccgat gaaggtgatg aacaccatgc tgtgctcggc cgacgcgaac aacaaaatcg
     9721 cgagtggaat ccaagcgacc gcgggaatcg ggcgtaacac ctcgagcgct gggcacagag
     9781 aataggccgc cagacggaat cgcccaatca ggagcccgag ccccactgcc aggctcgaag
     9841 cgattgcaaa gccgaagagg atgcggcgca ggctgtggag cacgtggtcg acgaacttcg
     9901 gcgccagaaa caagtccccc atcgctgcgg cgacctcggt cggggccggg acattctcga
     9961 agcgaaagaa gaagttccac ttatggctgg aagccacttg ccagagcgcc agccagacca
    10021 ccaatgagct cacgctgacc agccagggta cgagccggcg ccactcccga cgcagagctt
    10081 tgggccggcc ccgagcggct cgatcccaca tgattccgat tgcggagttt gccatgactt
    10141 agatgattca gagtatcggt tcggggaacc cgaagctcgc gaaggggctc caaggtccta
    10201 ttggaattgc cgttggaccg cctcgtagcc aatcacggct ccgccatgct gcgcggccca
    10261 ttgaaccgcc gcttccttcg agaggaaggc cgcgagccgg ccatgctggt ttacatacca
    10321 gaccttgtcc gcaaacagct tattgccact cagacgatcg tgcacgaacg cgacgcgcag
    10381 cgactttccc tccgcgcgga ggcgccggag cgcagagaag gtcgcctcaa tcgtgctgta
    10441 cagtcgaact ttcggctcac ccttgaccca gagttggccg gccaagcttg gctcttcgac
    10501 cttggcgtgg gtatcgatcg cttctcctgc aaacggcagg gcggtgtagt ccttgaggcg
    10561 cgcgtcatag tcgtagccga actctttggc ggcctggcgt atgaacttgt cattgacgta
    10621 gcgatcgacg tcgatctctt tgtccacctt tttcagcact ttcagcgagt cttgtgcccg
    10681 gcgaatcgcc gcgatgtact ctggcttcaa gctgtagtcg cgggtttgaa tgccttgagg
    10741 cccgtgaaag gcgtagtaga cctcggcgtc tacaccggtc catgtcgcca gcctctccga
    10801 caggccttcc ggatcttctc ggaacaagcg gtcggcctcc agagtcacct tcagataggc
    10861 taccaccaat tccggatact tcgtcgcgaa gtccgatcgc acctgcacgc cgtgggtggt
    10921 ggtcactccg gtcgagctac cgtcgtaaat cttcttcgca aaaccacgga aagggaacag
    10981 ctcaccgaaa ggcacgaagt tcgcatgcgc atcgatctgg tgcgcccgca gcgcgctacc
    11041 tgcgacctcg ggggtttgag tgacgatctc gacatccgat ttgggatccc agcccaaatc
    11101 ctgaaccgcc cggatcaaca ttgcatgcgc cgtcgatccg aacggcaccg agatcttctt
    11161 gtgacgaagt tcggagagtg actgcaccgt cgaatccgca ggcacgagga tcgcgttacc
    11221 ggcacctcgg atgccacccg ccaaactcgc gatgtagaaa gactccacgc cacccggcgc
    11281 ggccttgagt gccgaggctc caatcaccga cggaaagtca gccatctgca cgatatcgag
    11341 ctgattcgcc agatacttgc tgttcagttg tgccccagtg ggctgatttt cccaacgaat
    11401 gtcgtattga acgccggcgt agcgcccgtc gtgcggcagg taacgttcga gcaggtgcag
    11461 ctcgcgaatc agcggaccgc cggctgcaca gttgatcgtc gtgtcttgcg tgccaacggc
    11521 aatccgaatc cgcgcctctg ccccttcgcc cggcttgttc tttgccgtgg acgactcact
    11581 ggcggcgacg tctttcgctc gacatgccgg cagaagcagc gtgccgcaca gaaatatcaa
    11641 cgcatggaag gcgcggttca tgtcgaatct ctgagcctct cagttgtttc cattcaacgg
    11701 ttgcgtgtcg gccaacgctt gcaacaccga aacggcgcgt ttggccgcct tacgtacttc
    11761 gacgtcctcg tcgtgagcga gcgaacggag tctcggcaac gccgctcgca gccggattgc
    11821 gctgcacaat tcgatggcgg ctcgacgtac gtccgcgagc gcgctctcca gcagccgtac
    11881 ggcctcgggc gcggcgtctt cggcagcaca ggccgccagc gccaacaacg cttcacgttg
    11941 aacttgccac tgcgtgtctt gcgtgcgcgc gatgagcggc cctgcgagct cgcaggctct
    12001 gcgttgtccc aaggctcgcg ccgcgtcagc tcgcacgcgc caatccgagt cgttcaaggc
    12061 cctacctagc gcggagacca ccgcaggcga agatgcgtag tccagcgcac cgatggcggc
    12121 tctgcgtact cgtggctcgt cgtcagacaa tgcgccaatc aaggacggtg actccgcctg
    12181 cgaacgaagg aagcccaacg cgagcaccgc ctcgcggcgc acctccggat cggaatccgc
    12241 cagacgaagc accaatgccg acgccgcgag tggctcgcgc aggtcgcgca gggcaaggat
    12301 tactcctgcg cgtacgctcg cgagcggatg ctcgatcgcg cgcaatagtg cttccagcac
    12361 gctgggcccg cgcaggctac gcaaggcctc ccctgcccgc tcacgcacgt cgagatttgg
    12421 atctgcgagc agttcgatca gtggccgcgc gctgcgttgc tccccgacct cctccagcag
    12481 ctggaccgcc agagcacgca cggtagagtc gggatctcgg gtgcgcgcaa tcaaagcatc
    12541 cactaccgag gcatccaggg agccctccag cctcaacagc gctagttgcc gccgttcggg
    12601 gtctgggctt gcgagctccg acagaatctc gctcagctct tttgttcgag ttgactccgt
    12661 caagctcgtt gttcgcgtcg gctccgtcaa ctttgtcatt ggcgttgatt ccgtgagcat
    12721 tgtcttcgtt tatcgcgcct agcgtagcaa atatggtatc tgaacggtga tcgcatcttt
    12781 gggacaatcg atctcgcagg ccagacaata ccagcactcg tcgtagcgca tgtgtgcctt
    12841 gcccgacaca gggctgattg ccaagcaatc gacggggcaa ctgtcgatgc atacgcggca
    12901 gtttttgagg cacttctgtt catcgaccac gaccggaacg ctcacccgca gtgaggtttg
    12961 cgaaagcgtg gtcggctgct gggcgagggc tcgcgcatgg gcagccttga cgctttctcg
    13021 gtctccgagt tcactcatcg aatctctccg ctccgctatg acgttgggta gctaagaacc
    13081 gttctaagcg cttgcgagcg agagctcgtt gacgcgcaac ttgtgatagg ctctgagttc
    13141 gtccgaactc agtgacacta cgtatggctc gacgtcacgc ctgaaggctt ccatctcgcc
    13201 cgcagcagac tttcgcaagt tgacgtgtac gaaccaatct cggtcgttca gatcggggaa
    13261 gtcgaggcga tagtggtaga gcccccagcg gctctccgtg cggaacagcg aggctcgcgc
    13321 tgccatctcc gcacaatccc ggatgaagtg gcactccatg actcgcatga gctcatgcgg
    13381 gttggcagca ccgagttcgt cgagttcttc ccgggcacgc atgaactgcg caaggccgct
    13441 ctccatacgc ttcgctgttt ttggcggctg caggtagtcg ttgactaacc gccgtacctt
    13501 gtattcgtac tggtggtggg gcaacccgtg agggcgcgac aagggcgcca atacgcgagc
    13561 ccgctcctgt gccacggcgt cctcggacgg ctcactcccg atgcccgccg aagccaagaa
    13621 tcgtaccgcg ttctcggcgc aaatttggcc ataggtcagg gcgccgagca agtagttgtg
    13681 cggcacgcaa gccatgtcgc cagcgctgta gagaccgtca actgtggttt cgccccgctc
    13741 gttcacgaat acgcccgaag cactgtgccc gctgcacaat ccgatttcag aaatgtgcat
    13801 ttcgacccga tggctcgagt agctgtgtcc gcggccgtgg tgaaaccgcc cgcggctcgg
    13861 ccgctcggtg cgatgcaata cgccttcgat ctcggagatg gtctcgggtg ccagatgcgt
    13921 gagctcaaga tagaccggtc cattcgacga ttggagttcg cggtagaact cgagcatcat
    13981 ttgcccgctc caatagtcac tctctatgaa acggtgaccg ttcgcgttca cggt
//
LOCUS       DASTKU010000050         4308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_231563, whole genome shotgun sequence.
ACCESSION   DASTKU010000050 DASTKU010000000
VERSION     DASTKU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231563"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFQ61_01645"
     CDS             complement(<1..692)
                     /locus_tag="VFQ61_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953173.1"
                     /translation="MSTTTKLGASLRDVVLLSLVTQAACSCTSSGGAITGTGGVAAQG
                     GVAAQGGLAGQLGSSGESGGNTSVGSRGSLSGGMAGALSGGGVGGGGVGGGGGMSGAA
                     QGDNELFVPADLPNTLAEGEQGVQLKLHAFTLTPGALGLELYAAVKNEGDRPACQAGM
                     LTFFYDHSDQVVASAGSVLRAKQFYRIDTGDLILCIAPGEVAMSATNLPKELVISDVS
                     RLEHRFPAFTVDG"
     gene            1255..1776
                     /locus_tag="VFQ61_01650"
     CDS             1255..1776
                     /locus_tag="VFQ61_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953174.1"
                     /translation="MMFNTSAVFRLLLVTGGAATWAMVGCSGSEPTASNALSQEASIA
                     AALEATEGSLATAEEDAKSCFETYRACKVAEAADVQSCRAALKACLPAEAPAPRTCGG
                     DHPRHGGDGRPPATMAPVVQAASPSHQAEPPRLASAGSAEKMAPARTRPAVLEPRVPR
                     SAAVRDPSVHRLQ"
     gene            1839..2006
                     /locus_tag="VFQ61_01655"
     CDS             1839..2006
                     /locus_tag="VFQ61_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953175.1"
                     /translation="MDDATNAHRGCLQDAFGDHIAHLCEEAAKICQQSDAHRDLCARL
                     ADACQTLTATP"
     gene            complement(2147..3490)
                     /locus_tag="VFQ61_01660"
     CDS             complement(2147..3490)
                     /locus_tag="VFQ61_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159397871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2403 domain-containing lipoprotein"
                     /protein_id="HET9953176.1"
                     /translation="MNSSTTSARGGTSDGTAPAQGGTRPESLGGRPTSGGAPGRGGTS
                     AAAGTNSDAGHGSAAGASGGSAPQPDPSALGAYPIKDSPLTRGGTMTFTNIGAEGWWP
                     RRIDRAAGDPACDYKDGTDTWGGHCCMKKHATASDELSPFDQEMTLIFKAIDIKQLAV
                     YQPASPGVDYTRVSAWDARRGNDADNLWFTQQGEGSALFPGNLTHNDCVGYVMQQPSF
                     ECGDGKDYFCPNDPGVLHRGFSGSKLVVFLGSMSFSDGTVQACNGEGTGHPGPWVAFV
                     ASELVRDGGRKWNGACNCYSKTGSVGDGCGEINVFEVVMDNNDFSNREFASTGVRSFQ
                     AGHVGGAVCGSECRREQFASDADVVDACAHAAYSRGPEIVAGGKTDGCPVWRRPEGDR
                     YFFILLDQVTRTIQVGMVHPKAIPAALGAWLPELPNRVNRSDIDALAKLRLPSGG"
     gene            complement(3628..>4308)
                     /locus_tag="VFQ61_01665"
     CDS             complement(3628..>4308)
                     /locus_tag="VFQ61_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thaumatin family protein"
                     /protein_id="HET9953177.1"
                     /translation="GGMGGAGMGGNTSAGSSSAGNANAGNTSAGAASIDCNQSGDGKT
                     TLTFVNRCSKELTYSGSDIASGQLAPGAFACVDIGTATEAISSKRYWGFVGQDPGAEH
                     HTLAEFTFNTDFNDFDWYNISHVDAHNLPMQIVPVGQPGCKTLTCAQDFLSGCPSVGQ
                     YKDGSGAVIACVSPERDNGESVVARYFEACDDAYAWSADDQKGSDPSPMRACAGEDWN
                     IVFCPESP"
BASE COUNT          785 a         1429 c         1294 g          800 t
ORIGIN      
        1 ccgtccaccg taaaggccgg aaaccgatgc tctaagcggc tgacgtcgga gatcacgagc
       61 tctttcggaa ggttggtcgc agacatcgcc acctcgccgg gcgcgatgca aagaatcaaa
      121 tctccagtgt cgattcgata gaactgcttg gcacgtaaca cgctgcccgc actggccacg
      181 acttgatcgc tatggtcgta aaagaacgtg agcattcccg cctggcaagc gggtctatcc
      241 ccctcgttct tcacggccgc ataaagctcc agcccgagcg cgccgggcgt gagcgtgaaa
      301 gcgtgcagtt tcagctgcac cccctgctcg ccttctgcca acgtgttcgg taggtcggcg
      361 ggaacgaaca actcattgtc tccctgggcc gctccgctca ttccgccccc gccaccaacg
      421 cccccgccac caacgccccc gccactcaat gcgcccgcca ttccgcccga gagtgaccct
      481 ctcgatccaa cgctcgtatt gcccccgctc tcgccgctgc ttcccagttg ccctgccaag
      541 ccgccttgag ctgcaacccc gccttgagct gcaaccccgc ccgtgcccgt aatggctccg
      601 ccagagctag tgcaggagca cgctgcttgg gtgacgagag atagcagaac gacgtctcgc
      661 aacgaagcac cgagtttggt ggttgtggac attacggtgg tcgtgccgtc gcgggccatc
      721 gcagcccgtg caacatagca gcggataggt ccccacgcga gcaaactcat cagtcgttgc
      781 gccggcaaca tcgagtgctg ccgcgcaatc cgcacccgtc atggctgcgc cacccgcgaa
      841 ccgcggtact cccgctgggc cggtagcacc cgtcactccc aagcattgac cccacgaaaa
      901 ttggcccgac tatatcttgc gggccaatcg atctcgacgc gccgatccgt ctgtcactga
      961 cgcatcgccg atgacgagga ttcgtatcgt cagcagtgtc tcgtcgatgc ggcaattggc
     1021 ttctcgatgc ggcactgctg agcgctctcg tccatagcgg gggctcgtgc ctggcgcgga
     1081 gcggtatcag gcgaagcccg ttctccacga tttacggcgt ctcgagcgta gcctcggaaa
     1141 aagcatcggc ggcggtgatc gctctcatga aatcagatac ccaatctcca cgaaacaggt
     1201 cgtcgcagcc cggcagtcga actgcggggc gcggaggctt tgacactgga gcacatgatg
     1261 ttcaacacaa gcgcagtttt ccgtctgttg ctggttacgg gtggcgcagc cacttgggct
     1321 atggtcgggt gcagtggaag tgagcccacc gcgagcaatg ccttgtccca ggaggcaagt
     1381 atcgcggccg cgctcgaggc caccgagggc agcctcgcca cggccgagga ggacgccaag
     1441 agctgtttcg agacgtatcg agcttgcaag gtggccgagg ccgccgacgt ccagagctgt
     1501 cgcgcagcgt tgaaggcatg cttgcctgcg gaagcgcctg cgccgcgcac ctgcggcggg
     1561 gaccaccctc gccacggcgg agacggacgt cctcccgcca cgatggcgcc ggtggtgcag
     1621 gcagcgagcc cgagtcatca ggcggagcct ccgagattgg cgagcgcagg atcggcggag
     1681 aagatggccc ccgcgcgaac gaggccggca gtgctggagc cgagagtccc ccgctcggcg
     1741 gcggtcagag acccgagtgt gcaccgcctc cagtagaggg tggacgggtt cgcgcgtgcg
     1801 cagaggcggc cagcaccgcg ctcgcaacga gtgccgatgt ggacgacgcc accaatgccc
     1861 accgaggctg tttgcaagac gcattcggag accacatcgc gcacctgtgc gaagaagcgg
     1921 ccaagatttg tcagcaaagc gacgcgcatc gcgatctgtg cgctcggctc gcggacgcct
     1981 gccagacact caccgctacg ccgtgatcga gcggcttcgg caggtagaaa tacgataggc
     2041 acgttgcgcg ggaagagcgt ctctcggggg cgagcttccc gctgctcatt gagctagttg
     2101 gcgtagcgcg atacgcggca cactacgagc ctagattggg tggctctcag ccaccagaag
     2161 gcaaccggag cttcgccaag gcatcgatat ccgatcggtt cacccggttc ggcagctcgg
     2221 gtaaccatgc cccgagcgcc gccgggatgg ctttggggtg caccattcca acctgaattg
     2281 tgcgcgtgac ttggtcgaga aggataaaga agtaacggtc tccttccggt cgtcgccaaa
     2341 ccggacatcc gtcggtcttg ccgccagcaa cgatctccgg tcctcgtgaa taggctgcat
     2401 gcgcacacgc atcgaccaca tcggcgtcac tcgcgaattg ctcacgcctg cactcgcttc
     2461 cacaaaccgc accgcctacg tgcccggctt gaaaagagcg cacccccgta ctcgcgaact
     2521 cgcggttgga aaagtcattg ttgtccatca cgacttcgaa gacattgatc tcaccgcagc
     2581 catcccccac actgcccgtc ttggagtagc agttacaggc gccgttccac tttcggcctc
     2641 catcgcgcac gagctctgac gcgacgaacg ctacccaagg ccctggatgg ccggtgccct
     2701 ccccgttgca agcttgcacc gtgccgtccg agaaactcat cgagccgaga aacacgacga
     2761 gcttcgatcc cgaaaagccc cggtgcagca cgcccgggtc attcggacag aagtagtcct
     2821 tgccgtctcc gcactcgaac gagggctgct gcatgacgta gccgacacag tcattgtgag
     2881 tcaggttccc cggaaacagc gcgcttcctt caccctgctg agtgaaccaa agattatcgg
     2941 cgtcgttgcc gcgccgcgcg tcccacgcgc tcactcgagt gtagtctact cccgggctgg
     3001 cgggctgata tactgccaat tgcttgatgt cgatcgcctt gaagatcaga gtcatttcct
     3061 ggtcgaatgg cgagagctcg tcgctcgcgg tcgcatgctt cttcatgcaa cagtgcccgc
     3121 cccacgtgtc cgttccgtct ttgtagtcgc acgccgggtc gcccgcggcg cgatcgatcc
     3181 gccgcggcca ccagccctcg gcgccgatgt tggtgaaagt catggtgcct ccccgagtca
     3241 gcggcgaatc tttgattggg taggccccga gcgcgcttgg gtcgggctga ggcgcgcttc
     3301 ctccgctagc gcctgcggcg gagccatgtc ctgcgtcaga gttggttccc gcagccgcgc
     3361 tcgttccccc gcgacctggt gcaccaccgg aagtcgggcg tcccccgagg gactcggggc
     3421 gggttccacc ttgcgctggc gcggtgccat cgctcgtgcc gcctcgagcg ctggtagtac
     3481 tggagttcaa tctgccgccc acggacgttg cgccacctcg tgtggaggcg ccggctcccc
     3541 cagtgtctgt cgagtggcct ggattgctac aggcgagtgc caagccagcc aaaactcgtg
     3601 cccgattctg aactcggcca attagactca tggtgactcc gggcaaaaga cgatattcca
     3661 gtcttcacca gcgcaagctc gcatggggct cggatcagag cccttttggt cgtctgccga
     3721 ccaagcgtag gcatcgtcgc acgcttcgaa gtagcgcgcg acgacgctct caccgttgtc
     3781 gcgttcgggg ctaacgcagg cgatcacggc gccgctgccg tccttgtact ggccaacgct
     3841 gggacagccg ctcaagaaat cctgggcaca ggtcagcgtt ttgcaacctg gttgcccgac
     3901 gggcacaatc tgcatgggca ggttatgcgc gtccacgtgg ctgatattgt accagtcgaa
     3961 gtcattgaag tcggtgttga aggtgaattc ggcgagagta tggtgctcgg cgcctgggtc
     4021 ttgacctaca aagccccagt agcgcttgga ggagatggcc tcggttgcgg taccgatatc
     4081 cacgcacgcg aaggccccgg gcgcgagctg tccacttgct atatcgctac ccgagtatgt
     4141 cagttccttc gagcagcggt tcacgaacgt gagggtcgtt ttgccgtcgc cactttggtt
     4201 gcaatcgatc gaggccgccc cggcactcgt gtttcctgca ttcgcgttgc cagcactgct
     4261 gctacccgca ctcgtattac cacccatccc ggcgccaccc attccgcc
//
LOCUS       DASTKU010000051         8477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_231846, whole genome shotgun sequence.
ACCESSION   DASTKU010000051 DASTKU010000000
VERSION     DASTKU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8477
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231846"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1106)
                     /locus_tag="VFQ61_01670"
     CDS             complement(9..1106)
                     /locus_tag="VFQ61_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9953178.1"
                     /translation="MSPRIALCLPGGGAMGALYQIGALAAVEDAVEGFDANSFDLYVG
                     SSSGASLSAVLSAGVSVQRLYRAFLDPADVYFGLERKHLLRIDLGEWRRTLLTAMSAL
                     RHAAGSALTRESKALPSKLWQELDRLYDSLPAGLFTLEGYERFLEDFFVRRGVPNVFA
                     AMPRPLYVLSHELESGAPVCFGSPLQSDVPVTRACIASMAIPPFFSPVRIGDKQYINP
                     GAGQISHLDVAVEAGAEVLFVVHSMVPVCVESVPTGHGPRTSLRDKGMMWVLNQGIRI
                     GMQRLVDEATQRVVRDRGVAVLRIEPEPADGILFMDNSASFEARRRLLEHAYRSTRLE
                     MARRFAAGDESLQRACLKPRQNPRTVSPPAA"
     gene            complement(1103..2020)
                     /locus_tag="VFQ61_01675"
     CDS             complement(1103..2020)
                     /locus_tag="VFQ61_01675"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9953179.1"
                     /translation="MSQLIAGALALALSVSLVLLTATGFTLLASVWPQAAQHLFAAAG
                     VELVTYGIGSACLLTWLRPRTKLWSLRRLSFGVLVCSALAGVALHGPLDYIEHWVEHL
                     LPLSDAVWSERAARLSPPSAGERALLMIAVAGVVPLVEEYFFRGALFELLGRSRGSAH
                     VVPWAAANPGLAPLESRSALPRANSSFGEWAFPIGLSSVCFALSHGEPRNWPALLLVA
                     GVMGLLRVASGSLYPSILLHAAFNATTLVVSYSTSVSSHTRATPGLGGALLGSCVSGL
                     LLAFALSSRKSRGERRASGEQFETKDGVR"
     gene            complement(2067..3674)
                     /gene="nadB"
                     /locus_tag="VFQ61_01680"
     CDS             complement(2067..3674)
                     /gene="nadB"
                     /locus_tag="VFQ61_01680"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HET9953180.1"
                     /translation="MSEKTDYLVLGSGVAGLSFALHAAEHGEVLLLTKREPGDSATSW
                     AQGGVAAVLSPEDSFERHAEDTAVAGAGLCHDVVVDLCVREAPAAIRWLADLGARFSE
                     DPAGEFDLGREGGHTQRRIVHAGDITGREIQRALWEAVRASPNIRVLDWHMGIDLITL
                     SNFGGPDRCVGAYVLDERRGVVKTILARATVLATGGAGKVYLYTSNPDVATGDGVAMA
                     YRAGAEIANMEFYQFHPTVLYEPKAKSFLVSEALRGEGGILRLPDGEAFMRRHHPMAD
                     LAPRDVVARAIDYEMKRTGSENVLLDMTACAPDFLRNRFPNIYAECLHYGIDITVQPL
                     PVVPAAHYMCGGVTSDLHGRTTLPGLWVVGETACTGLHGANRLASNSLLEGLVFARRA
                     ASRLASQISELRTQLSPRVPEWQTGSAVPSDEAVIVSHNWDELRRTMWNYVGIVRSDA
                     RLRRAARRVALFEQEIREYYWKHLVTRDLLELRNIATVAELIIASASSRHESRGLHYT
                     TDYPETSESYVADTVMKRGVLPHLRGH"
     gene            3851..4681
                     /locus_tag="VFQ61_01685"
     CDS             3851..4681
                     /locus_tag="VFQ61_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET9953181.1"
                     /translation="MKVLVFVKRVADPDNANKLKIAASGNAVDTSGLEWKLNPFDEYA
                     LEAALRLTENGKAPKSRLGEVVVATLGPKEVETNLRSAMATGADRALRVDATDDALDG
                     LLVSRVMQKLVELEKPDLVLMGKQAVDGDSNQVGQRLAELLDWPMATFAATLREQSDG
                     KLLVGREVDGGVSNVRLTLPAVVTVDLRIVASASVYSKHTDAGFKYNDGVRFAALPAI
                     MAAKKKPLEVKSLAELAPGLGLTTTYHRFERPAARKAGIKVKDVSELVQRLTAEAKVI
                     "
     gene            4692..5666
                     /locus_tag="VFQ61_01690"
     CDS             4692..5666
                     /locus_tag="VFQ61_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET9953182.1"
                     /translation="MGNVLVVAELGEDQKLKKSTLSAITFAKAALPALGGSFTILVLG
                     SGVGEAAAELKGYGAARILVVDDSSLSHYLAESFTPTVAEVAKDYALLVATASSFGKD
                     LMPRVAARLGAAYAADCAGVSAQAGKLLYKRPMFAGNAFGYCTLETPIQVATARQSEF
                     TPAEPSGGDSPIEAVAVRGRSQASSRIEFVGLDAVKSERPELSDAKIVVSGGRALKEK
                     FFEVLEPLADSLGAALGASRAAVDAGYAPGDFQVGQTGKIVAPALYIAVGISGAIQHI
                     AGMKGSRVIVAINKDAEAPIFQLADYGLVGDLFQVVPALTQALKQRAT"
     gene            5826..5899
                     /locus_tag="VFQ61_01695"
     tRNA            5826..5899
                     /locus_tag="VFQ61_01695"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5860..5862,aa:Pro,seq:cgg)
     gene            6973..7581
                     /locus_tag="VFQ61_01700"
     CDS             6973..7581
                     /locus_tag="VFQ61_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9953183.1"
                     /translation="MAIEVPSVRILIVDDDRAICDYMQTLLEKDGFVVKTLSDPTLVE
                     DEVRQGDYHVMILDLMMPKMDGIEVLKRIRSVDSDIAVIIFTAHPNLETAIASMKLDA
                     VDYIKKPFNVDEFREVLSRVMKKKGLARTPEEQLHKIIGDTIRTLRKEKELTLKQMSR
                     RTGLSISLLSQIERAESSPSISSLYKIAIALDSKIHDLFGNN"
BASE COUNT         1712 a         2700 c         2623 g         1442 t
ORIGIN      
        1 ggcgtcgatc atgcggcggg aggcgagacc gtgcgcgggt tctgccgggg cttgaggcaa
       61 gctcgctgca gcgactcgtc tccggctgcg aagcgcctgg ccatttcgag gcgagtcgat
      121 cggtaggcgt gctcgagcaa ccggcgccgc gcctcgaaac tcgcggagtt atccatgaac
      181 aagatcccat cggcgggctc gggctcgatg cgcagcaccg ctaccccgcg atcgcgcacc
      241 acgcgctggg tagcctcatc gacgagacgc tgcatgccaa tgcggatgcc ctgattgagc
      301 acccacatca tgcccttgtc tcgcaggctg gtgcgtggcc cgtgaccggt cggaacgctc
      361 tcgacacaga ctggcaccat cgagtgcacc acgaacagaa cctcggcgcc ggcctcgacg
      421 gccacgtcca aatgcgaaat ctggcccgcg cccgggttga tgtattgctt gtcaccgatg
      481 cgcacgggcg agaaaaacgg tgggatcgcc atcgaggcga tgcacgcgcg ggtcaccggg
      541 acatcactct gtaaggggga tccaaaacac accggcgcgc cggattcgag ctcgtgtgaa
      601 agcacgtaaa gcgggcgcgg catcgcggcg aacacgttcg gtacgccccg acgcacgaag
      661 aaatcctcca gaaagcgctc ataaccctcg agcgtgaaga gcccggctgg caacgaatca
      721 tagagccggt cgagctcttg ccaaagtttc gagggcaacg ccttcgattc acgggtcaag
      781 gcgctacccg cggcgtggcg taaggccgac atggcggtga gcaacgtgcg tcgccactcc
      841 cccaaatcga tgcgcagcag gtgcttccgc tcgagcccga agtatacgtc cgccggatcc
      901 aagaacgcgc ggtagagacg ctgcacggat acacctgccg acaatacggc cgagaggctc
      961 gcgccactgg agctgcccac gtacagatcg aaggagttcg cgtcgaagcc ttcgacggcg
     1021 tcttccactg ccgccaacgc accaatctgg tagagcgcac ccatcgcgcc gccacccggt
     1081 aagcacaagg caattcgcgg gctcatctaa caccatcctt ggtctcgaat tgctcgcccg
     1141 atgctcgacg ctcccctcgg ctcttccgtg agctcaacgc gaaggcgagc agcaacccgc
     1201 tgacacagga cccaagcaga gctcccccga gaccgggtgt ggctcgcgtg tgcgagctga
     1261 cggaagtcga gtaactcacg acgagggtcg tcgcattgaa cgcggcatga agcaagatcg
     1321 aaggatagag ggagcccgac gctacgcgca gcaagcccat cacccccgcc accagcagca
     1381 aggcaggcca gttgcgaggt tcgccgtggc tgagagcaaa gcaaacactg ctgagcccga
     1441 tggggaaagc ccactcaccg aacgaagaat tggctcgcgg cagcgcggac cgcgattcga
     1501 gcggagccag cccagggttg gccgccgccc agggcaccac gtgggctgag ccgcgcgatc
     1561 ggccgagcag ctcgaacaat gcgccgcgaa agaagtactc ctccacgagc ggcaccacac
     1621 ccgcgaccgc tatcatgagc agcgcccgct ctccggctga cggcggagaa agtcgcgcgg
     1681 ctcgctcgct ccagaccgcg tcggacagcg gcaagagatg ctcgacccaa tgctcgatgt
     1741 aatcgagcgg accgtgcaac gcgacccccg ccagagccga gcagaccaag accccgaaag
     1801 agagccgacg caaagaccag agcttggtgc gcggccgcag ccacgtgagc aagcaggccg
     1861 aaccaatccc gtaagtcacc aactcgacgc cggcggccgc gaacaggtgt tgcgcggctt
     1921 gcggccacac gctcgcgagc agcgtgaacc cggtcgcggt aagcaacacc aagctcacgg
     1981 atagcgcgag cgccaaggcc ccagcaatca gctgcgacac gagaggctcc gcccgtggga
     2041 agccgcgccg aactcaacgc gggacgtcag tgccctctca aatgcggaag cacaccgcgc
     2101 ttcatgaccg tgtcggccac gtagctctcc gacgtctcgg ggtagtcggt cgtatagtgc
     2161 agcccccggc tctcatggcg gctcgaggcg gacgcgatga tcaactccgc gacggtcgcg
     2221 atgttgcgaa gctccagtaa gtcgcgggtg acgaggtgct tccagtaata ctcacgtatt
     2281 tcttgctcga acagcgccac gcgccgtgcc gcgcgccgaa gacgtgcatc gctccgcacg
     2341 attcccacgt agttccacat cgtgcgccgc aactcgtccc agttgtggct cacgatcacc
     2401 gcttcgtcac tgggcacggc tgaacccgtc tgccactccg ggacgcgcgg actcagctgg
     2461 gttcgtagct cggagatctg ggaggccagc cgcgatgccg cacggcgcgc gaacaccagc
     2521 ccctcgagca atgaattgga ggccaggcga ttggcgccgt gaaggccggt gcaagccgtc
     2581 tctccgacca cccaaagccc cggcaaagtc gtgcggccgt gcaagtcgga ggttacgcca
     2641 ccacacatgt agtgggccgc gggtaccacc ggcaagggtt gcacggtgat gtcgatgccg
     2701 tagtgcaagc actcggcgta gatgttgggg aaacgattgc gaagaaagtc cggggcacac
     2761 gccgtcatat ccagcagcac gttctccgaa ccggttcgct tcatctcgta gtcgatggcg
     2821 cgtgccacca cgtcgcgcgg ggcgaggtcg gccatggggt gatggcgacg catgaacgct
     2881 tcgccgtccg ggagccgcag aatcccgcct tcgccacgga gcgcctcgct cacgagaaag
     2941 ctcttggctt tgggctcgta caacaccgta gggtggaatt ggtaaaactc catattggcg
     3001 atctcggccc ctgcccgata cgccatcgcc accccatctc cggtcgcaac atcggggttc
     3061 gaggtgtaga ggtaaacttt accggccccg cccgtggcca acaccgtggc tcgcgccaaa
     3121 atggtcttca cgaccccacg tcgctcgtcg agcacgtaag cgcccacaca gcgatccggt
     3181 cccccgaaat tcgagagcgt gatcaggtcg atccccatgt gccaatcgag cacgcgaatg
     3241 ttgggtgacg cgcggacggc ttcccacagc gcccgttgaa tctcacggcc cgtgatatca
     3301 ccggcatgga cgatgcgacg ctgggtgtgg ccgccctctc gcccgaggtc gaactcaccg
     3361 gctgggtctt ctgaaaaacg ggcgccgagg tcggccagcc accgaatcgc cgccggcgcc
     3421 tcacgtacgc acagatcgac gaccacatcg tggcacagcc cagcacctgc gacggcagtg
     3481 tcttcggcgt ggcgctcgaa cgaatcctcg ggagagagta cggccgccac tcccccttgc
     3541 gcccagctgg tcgcggagtc cccgggctca cgcttggtca gcagcagcac ctcgccatgc
     3601 tcggccgcgt ggagcgcgaa cgacaagcct gcgaccccgc tgcccagcac tagatagtcg
     3661 gtcttttcgg acatcagcag gggctacata ccaccgagat gctctagaac ctatccggac
     3721 ctctcggaac ggactgggca gttcgacccc ttcggcccgt ggacccagca tcgatcctgc
     3781 gcaggtcccg agtttgccgc tgaaccgcga gtggggcagt cttcctttga ctcccaagga
     3841 gcaagccacc gtgaaagtac tggttttcgt caagcgtgtc gcagacccag acaacgcaaa
     3901 caaattgaag atcgccgcga gcggaaacgc ggtggataca tccggactcg agtggaaact
     3961 caacccattc gacgagtacg cgctcgaggc cgccctacgc cttaccgaga acggtaaggc
     4021 ccccaagagc cgtttgggtg aagtcgtagt cgccacactc ggtcccaaag aggtggagac
     4081 gaacttgcga tccgcgatgg ccacgggggc cgatcgcgcg ctccgagtgg acgccacgga
     4141 tgacgcgctc gacggcttgt tggtctctcg ggtgatgcaa aagctcgtgg agctggagaa
     4201 acccgacttg gttttgatgg gtaagcaggc cgtagatggg gacagcaatc aagttggcca
     4261 acgcctcgcg gagctcctcg attggccgat ggccacgttt gccgcgacgc tgcgcgaaca
     4321 gagtgacggg aagttgctgg tcgggcgcga ggtggacggc ggagtgtcga acgtacgact
     4381 gacgttgccc gccgtggtca cggtggactt acgcatcgtg gcgagcgcga gcgtatattc
     4441 gaagcacacc gacgcgggct tcaagtacaa cgacggggtt cgtttcgcgg cgctgccggc
     4501 catcatggcc gccaagaaga agccgctcga agtaaagagc ttggcagagc tcgcccccgg
     4561 cctcggcctg accacgacct accaccgctt cgagcgacca gcggcgcgca aagcgggcat
     4621 caaagtcaaa gacgtgagtg aactcgtgca gcgcttgacg gccgaagcca aggtcatttg
     4681 aggagaagaa aatgggaaac gtactagtgg tcgccgagct cggagaagac cagaagctca
     4741 agaagagtac gctctctgca atcaccttcg cgaaagcggc gctcccggcg ctgggaggat
     4801 cgttcaccat cctcgtgctc gggtcgggtg tcggcgaggc agccgcagag ctcaaaggct
     4861 acggagcggc ccgcatcctc gtcgtggatg attcttcgct gtcgcactac ttggccgagt
     4921 cgttcacccc gactgtggcg gaagtagcca aagactacgc tctgctggtg gccaccgcgt
     4981 cgagctttgg gaaggatctg atgccccgag tggcagcgcg gctcggagcc gcttacgcgg
     5041 cggactgcgc tggggtctcg gctcaagctg gcaagctcct ctacaagcgc cccatgttcg
     5101 ccggcaacgc cttcggctac tgcacgctcg agacgccgat ccaagtcgcc accgcccgcc
     5161 aaagcgaatt cactccggct gagccgagtg gtggggacag ccccatcgaa gccgtcgctg
     5221 tgcggggccg ctctcaggcc tccagtcgca tcgagttcgt cggcctcgac gcggtcaaga
     5281 gcgagcgacc ggagctgtcg gacgcgaaga ttgtcgtgtc gggtgggcgc gcactgaaag
     5341 agaagttctt cgaagtcctc gagccgctcg ccgactcctt gggcgcggcg ctgggagcaa
     5401 gtcgcgccgc cgtggacgcg ggttacgcgc ccggggactt tcaggtcggc caaacgggaa
     5461 agatcgtcgc acccgccttg tatattgcgg tcggcatctc cggagcgatc cagcatattg
     5521 ccggcatgaa agggtcccgc gtgatcgttg ccatcaacaa ggacgccgag gcgccgattt
     5581 ttcagctcgc ggactacggc ttggttgggg atctcttcca ggtcgtgcca gcgttgaccc
     5641 aagctctaaa gcaacgcgcg acctgacttg gttcgtgggg ccgccggcat cgggcccagt
     5701 agaggcgggg aaccgccggg cccagcggtg gcgaacgaaa aaacgtcgaa tcgcacctgt
     5761 cagcgcttga cagaaaagcc tgaaaagaac atgttccggc gcccctcgac gaggggtcgc
     5821 ctgttcgggg cgtagcgcag tttggtagcg cgcacggttc gggtccgtga ggtcggcagt
     5881 tcgaatctgc ccgccccgac aactagaaaa cagatagctc gacgttcgct acagagctcc
     5941 gcctgcaagc tgtccgcgag gacgcagcag gcgtcccaac ggggattcgt ttctgtgcag
     6001 gctcgggtac gctgcttcgc acgagcgtga cggcccgatt catctgtcga tggtagtcgg
     6061 caacgtcgat gacagcagct ccgcaagcca agcgctagcg agggagcgag ggcaccgtaa
     6121 ggcggttggg aaggccggcg ctctacggca ccggtccagc acaggcacgg ggtcacggcg
     6181 tgagatgtta cggcccccgc gtaaccccaa cggctttcgg ttgcctctgg atcgcataac
     6241 tcgccgccat ccatggcaga gcctccacag ccagcttcgc cggtgacgag agacatcgaa
     6301 agcgcgtcgt ctgcacgcgg gcgtcgtcac gcgcagcgcg ttgcacgctg caccgcgaga
     6361 tgatgcaaaa gctgcacgcc tcgagcccgc ccgccgttaa tcaacctcga ggatgatgtg
     6421 gcggaagctt tcgagctgac ctcgagcctg atgactacga agtcacgcga gtacacctcg
     6481 tcgctccgcc attggtgaga tctcttcgac agcgcggcca tctcagagag gattcactca
     6541 ctcagatcgc gttttgaatc atgatgctca gcgcgacatg gggaacgtag ccacaaggtt
     6601 acgcgcacga ggataggcaa cagccttgat tttcagcgtc aaacccacct tcgaggccaa
     6661 cttcaaactg gcgcggaccg gaaccgtaga agccagtgga cagcgctgtg acacgatcgc
     6721 tcgcggaccc agacgccaat ggttttcgga atccgccagg aatgtcgtga ccggtcgatc
     6781 catggtcacg tgaactagaa cgcttgacga gacccgataa tccggccagc tgttctgctt
     6841 attagccagt aatttcagtg agttgcagct ccagaaacga aaggcaaaaa tctagtggag
     6901 tggtaacccg gtcggtatta tagtgtcact gaaagacggc aacttgctgg actacgcaaa
     6961 aggagcacgc ccttggcgat cgaagtaccg agcgtccgaa ttctgatcgt cgacgacgac
     7021 cgcgcgatct gtgactacat gcagaccctg ctcgaaaaag acggattcgt cgtcaagaca
     7081 ttgagcgacc ccacgctcgt ggaagatgaa gttcgtcagg gtgactatca cgtcatgatc
     7141 ttggatctca tgatgccgaa gatggatggc atcgaggtcc tcaagcgcat ccgctctgtc
     7201 gacagtgaca tcgcagtcat catcttcacg gctcacccga acttggagac ggccatcgcg
     7261 tccatgaagt tggacgcggt cgactacatc aagaagccgt tcaacgtgga tgagttccgt
     7321 gaggttctct cgcgcgtgat gaagaagaag gggcttgctc gcacgcccga ggagcagctc
     7381 cacaagatca tcggcgacac gattcgcacg ctgcgaaaag agaaggagct cacgctcaag
     7441 cagatgagcc gccgcactgg gctgagcatc tcgcttttgt cgcaaatcga acgcgccgaa
     7501 tccagcccat cgatctcgag tttgtacaag atcgccatcg cgctcgactc caagatccac
     7561 gatcttttcg gcaacaactg acgctcgtcg cgtgggtttg gttcgaggtt gccagtagcc
     7621 aaacgtccgt tgccgtgttt gcggccgcgt gtctcccttc gcgcggccgc ttgcgcaacc
     7681 gttgctgccg gatcgatccg cgtgaggttg cgttctggtc cagctccgtc cgcgccatgt
     7741 atcgctgcct ctatggtcga atcgctggac gagacccgtt gactcgggcg acggttgctg
     7801 agttctaagc ggaccggcga gcctggcttc tctcgaatca gcggttgatc ctggcgttcg
     7861 agacggccgc ggcatatccc gttggacgcc gcctgcaagg cgtgggactt cgaacagcct
     7921 ctacgaatcg tgcttgtttc cgatttggcg ctcgcgagtt gcggcacgcg agttgcgctt
     7981 caagcagcac tctcggtcga ctcagcagca gcgagttgca accacgttca ttcgcggcct
     8041 cacctacccc tcacgcttcg cctctgagtt cagctcgtgg ctcttggccc gatcctgccg
     8101 cgtcaccgct ccgatagcgg cgctgcttcg agcgacttca ctcgcgggtt aagctgccaa
     8161 cctcaaaccg aagttactgc cggcttcgaa cgcggagctc cccaccagga ctaccctgcg
     8221 tcgaagtacg acccgtcgat gcctctcacg tcctagcgcg cagcgcttgc tttgttctcg
     8281 cgcagcaaca gcgagcgcct tcagcctggg ctcactcgcg taggcgacga tcttctcttc
     8341 gggccagacg gctcaaactc agcgtcgggc ggttattaat gcagggcccg caccgcaggc
     8401 ggggggttgt ttgggggggt gtgtatgggg ggcaaaccgc ggtacggacc ctgcaccgtt
     8461 actcttacca cgcgctc
//
LOCUS       DASTKU010000052         7612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1570580, whole genome shotgun sequence.
ACCESSION   DASTKU010000052 DASTKU010000000
VERSION     DASTKU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570580"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFQ61_01705"
     CDS             complement(<1..295)
                     /locus_tag="VFQ61_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9953184.1"
                     /translation="MRLARLELAAFGPFTERTLDLSGGAPAGLHLIYGRNAAGKSSAL
                     RAVSDLLFGIPAKSGDDHTHPYASLRIRALLESASGDSLFVQRLKRTKDSLRDA"
     gene            complement(292..1563)
                     /locus_tag="VFQ61_01710"
     CDS             complement(292..1563)
                     /locus_tag="VFQ61_01710"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HET9953185.1"
                     /translation="MKFVHAADLHIDSPLRGLERYQGAPVELIRGATRRALEHLVDLC
                     LEEGAKLLVLAGDLYDGDWRDYSTGLFFCGQMARLREAEVEVVWIRGNHDAASRLTQH
                     LHPTDNVRELSHTRAVTLEREIAGVPVAIHGQGYSKPKVTEDLADTYPAPVPGALNLG
                     LLHTALDGREGHDAYAPCRVEALLNKGYDYWALGHVHQREEVRREPCWIVFPGNLQGR
                     HARETGRKGASLVTVQSGRIAAVEHRVLDEVRWVHLVFDAGAAASADDVVDGVRDRLL
                     SEIKDADGRLVAARVSVVGASRAHAALSVDTERFQNELRAAALDVGRDAAWIEKVVLS
                     TRPEGDPVLLRARNDPLGGLLRALDKLRRDEGELSALCEELGELKRKLPRELLDGPDG
                     ARIGDAAGVRALLDDVESLLLPLLSEEASEP"
     gene            complement(1663..2349)
                     /locus_tag="VFQ61_01715"
     CDS             complement(1663..2349)
                     /locus_tag="VFQ61_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9953186.1"
                     /translation="MLIVSRWLGYGLLVFSLGAMLGCSSEAAKQETEPVSRCGLPLDT
                     SPRLGPDDAPVTILEFVDFQCPFCAAAEPIRDEVDAARPGLVRWVFKNLPLAGHARAM
                     PAALAAACAHEQGKFWPMHDLLFADQHAAEDADFLAYARQIGLDVAVWQACLASDRPR
                     ARVTADLTLAEQAQIGMTPSYLINGIRYPGLQQRQNLLAAIDRANAQVEESGLASAQY
                     YESLVAQGCQ"
     gene            2977..3954
                     /locus_tag="VFQ61_01720"
     CDS             2977..3954
                     /locus_tag="VFQ61_01720"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET9953187.1"
                     /translation="MSVTRLSAFAVLSGLSFLLVNCSSSTPASPVGGTGGSTPAGGTS
                     TATGGVTATGGAATSGGMSSSGGSNGGGATQAGASSGGSSVTGGATATGGNSTSGGTS
                     AIAGSSSGGTSTAGGAGNGGTSTVGGASGGASAGTSAGGAVGGSGGATGGTGGGSSGG
                     PFKVTSPDHEDGAKFGTAFTCAAMNGMFGSGVNPELNWTGVPAGTKSFAITFIDTKLG
                     ENSAMGQHWAAWNIPATAMQLPKGTTSFSGPLMGAKQTNKYLSPCPSGNDTYEFTVYA
                     LPVESLSVTGAEGTGVANSAGVAKVLAALKMANPLGNAKLSGTSGPMGK"
     gene            4211..5692
                     /locus_tag="VFQ61_01725"
     CDS             4211..5692
                     /locus_tag="VFQ61_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953188.1"
                     /translation="MRTVIGILGWALAVASCGESTEASPHGSGGSSGSQPGPNRASTG
                     GAGNETGDFPSGATSLAGLSSIGGAHSTTAGSTAASSGGDGANGSASPTRGGAHNSGG
                     APSSGGRAPISGGENAAAEAGAGGESSAAGAPPFEGAQRRVKELDLGGAHSCVRLADG
                     KVVCWGANEAGQLGDGALGNRNTAAPVHGLNNAVSVGTGTAHTCATLTDGTVTCWGLN
                     AHGQLGDRAPVNSIIPQSVPGVSAVVMVSAGAWHTCALRANGTVYCWGDNSHGQIGLG
                     RTSPDIAPPTLIPSLESVVRLAASADQTCALLETGAIYCWGNNDFGQLGDGTRLLDRA
                     APVPVQLTGHAIDVAVGARHACALLHDGSVQCWGGNSDGGLGDGTVEPSLLPVTVRGV
                     RGATQLALGDYHSCALSSDGKLRCWGYNALGQLGDGTQTNRNTAVLVSSLSAVVEVAA
                     GSVHTCALNRDDQVACWGLNYDGELGDESNSNRSSPVFVVGME"
     gene            complement(5798..>7612)
                     /locus_tag="VFQ61_01730"
     CDS             complement(5798..>7612)
                     /locus_tag="VFQ61_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953189.1"
                     /translation="CDVAWDRGGAAFIAGTICAPGVSYYSGILRRDPDGKLTHIAGNT
                     ASNGSTSEGVPATLAYLSSYHSLAFDPAGNLYVSQPGEAKVRRIEGASGRITTVVGTG
                     ASAFGGEYASAASAALNNPQGLSSDPSGNLYVVDTNNLSVRQVYRGANTIPSTATLSL
                     QSGDTQTATMGAMLGAFSAKLVDGSAAPLKGFNVAWRAVDFGAGLYAPSSTTGVTGIA
                     TATARTGLSPGAYRYEASFRDIHGQHVSGSPVLFSATASTPTSGTIFTLVNINTAESY
                     AGVPGPGPFAGISRPRDVAAGSDGTLYFVAGCAVYRLSKVGHLEIIAGGDLCGFAGET
                     GPALSAKFYAPGALALDEKNGLIYVSDTYNHRVRVVNLADSTIETFAGGGTAIGPNFG
                     DGGPATAASLNMPGHIAVDADGNVYFDDVNNDRLRRVDKATRIISRWVSYITCNGTNT
                     GLASCGSSGNGCDIAFDSNGNAYISSGYMCAPGVNSQFGVVKRAGNGTFTHVAGGTLA
                     WTEGMLATKASLVNLTNIALDSANNLYISQGGSNLLRRIDLSSGKIFSAAGDGAGGYA
                     EYVPAVSARVNYPLGLAAHPNGGVVFADYNNFAFRRIY"
BASE COUNT         1465 a         2432 c         2404 g         1311 t
ORIGIN      
        1 ttgcgtctcg cagcgagtct ttggtgcgct tcaggcgctg aacgaacagg ctgtccccac
       61 tggcactctc gagcagcgcg cgaatgcgca gcgacgcata agggtgggta tggtcgtccc
      121 ccgactttgc ggggataccg aacaacagat cggagacggc tcgcaatgcc gagctcttgc
      181 cggccgcgtt tcgcccatag atcaggtgta gccccgcggg ggctcctcca gacaagtcca
      241 gagtacgctc cgtgaagggt ccgaaagccg cgagttcgag ccgggccaac ctcaaggctc
      301 gctcgcctcc tccgacagca acggcaggag caaagattcc acgtcgtcga gcagcgcccg
      361 cacgccggct gcgtcgccaa tgcgcgcccc atccgggccg tccaggagct cccggggcag
      421 cttgcgcttg agctcgccga gctcctcgca cagcgcgctg agctcaccct cgtcgcggcg
      481 tagcttgtcc agcgcacgca gcagccctcc caacgggtca tttcgagccc ggagtaatac
      541 cggatctccc tctgggcgcg tgctcaagac cactttttcg atccacgccg cgtcgcgccc
      601 gacgtccagc gcggccgcgc gcagttcatt ctgaaaccgc tcggtgtcaa cgctgagcgc
      661 ggcgtgagcg cggctcgcac caaccacgct gactcgcgcc gccaccagcc gcccatccgc
      721 gtctttgatt tcgctcagga gtcggtcgcg aacgccgtcc accacgtcat cggctgaggc
      781 agccgctccc gcatcgaaca ccaagtgcac ccagcgtact tcgtccaaca cgcggtgctc
      841 caccgcggcg attcggccac tctggacggt caccaaagat gcacctttgc ggcccgtctc
      901 gcgggcatga cgtccctgga gattgccggg aaaaacaatc cagcacggct cacgccgcac
      961 ttcttccctt tggtgaacat ggccgagcgc ccaataatcg tagcccttgt tgagtaatgc
     1021 ctcgacgcgg cagggagcat aggcgtcgtg accctcccgg ccgtccaacg cggtgtggag
     1081 tagcccgagg ttgagcgcgc cgggcacggg cgcgggatag gtgtccgcga gatcctcggt
     1141 gaccttgggc ttcgagtagc cctggccgtg aatcgccact ggcacgcccg cgatttcccg
     1201 ctccagggtg accgcacgcg tgtgcgagag ctcgcgcacg ttgtcagtcg gatgcaggtg
     1261 ctgagtcaga cggctcgccg catcgtggtt gcctcggatc cagaccacct cgacctcggc
     1321 ctcgcgtaaa cgggccattt gtccgcaaaa aaacaggccc gtcgagtaat ctcgccaatc
     1381 tccgtcgtac aggtctcccg ccaggaccaa cagcttggcg ccctcttcca ggcacagatc
     1441 caccaagtgc tcgagggcac gtcgggttgc tccccgaatc agctcgacgg gggctccctg
     1501 atagcgctcc agcccacgca aagggctgtc gatgtgcagg tcggcggcgt gaacgaactt
     1561 cattcgagcg ccgcatgagt agcacgtcga ggagaccgtg gggccgatgt tgaatgttcg
     1621 gagccaaacc ccggccgtcg gggttcggcc tcgagcgcga gcctactggc agccttgagc
     1681 aactaacgat tcatagtatt gcgcggacgc gagcccgctc tcttccactt gcgcgtttgc
     1741 acggtcgatc gcggccaaca ggttctggcg ctgctgcagc ccaggatacc tgatcccatt
     1801 gatcagatac gacggtgtca ttccgatctg cgcctgctcg gctagcgtta gatcggcggt
     1861 cactcgcgct cggggccggt ccgaggccaa gcaagcctgc caaactgcta catccaaccc
     1921 aatctgccgt gcgtaggcca ggaagtccgc gtcctcggcc gcgtgttgat cggcgaacag
     1981 gaggtcgtgc atcggccaga acttaccctg ttcatgcgcg caagcggcgg ccaaagcggc
     2041 gggcatcgcg cgggcgtgcc ctgccagggg caggttcttg aacacccatc gcacgaggcc
     2101 cgggcgtgcc gcgtccactt cgtctcgaat cggctcggct gccgcgcaga acggacattg
     2161 aaagtccacg aattcgagga tggtgacggg agcgtcgtca gggcccagcc gcggggacgt
     2221 gtcgagcggc aggccgcaac gactcaccgg ctccgtttct tgcttcgcag cctcggagct
     2281 gcacccgagc atggccccga gagagaacac cagcaatcca taccctagcc agcgcgacac
     2341 gatcagcatg ccgccattgt agacgattcc tccccgaggc catggtcacg aggcatgaca
     2401 ttcgcctcga gcgccgcgat tgtcatgcca accctcgtgg attgcccgaa tgcactgtcc
     2461 ggagcacccc ctaggccagc caccggcaga ccgcttctcc acccagtcga agccaggctc
     2521 ggcttgctgc tgcatccgcc gcaatcaact cctccaaaca tcgtatcgaa caggagggtt
     2581 cccaagatgc attcgcattg caactagagc gacgattcgc ggaaggccgg tcgcacctcg
     2641 cgaccccaaa ccacggcgct atcccaaacc acggcgcgag ccatctgctc gatgaccgga
     2701 agtcgccaag cttagaattg aagagcttcg aacgtattat cgaaacagat cggtgcgtcg
     2761 aaagacactg cacctgcgag ggggggggct tgtactccaa catcgagact gcaaagttcc
     2821 cagtcccggc taaggcgttt tgtatttggt ctttcgatcg aaatctcacg agaaaaatcg
     2881 cctttaggtc gcgttctttg ctcgcaaaca cagatactca cgctatccgt tagcggtcgc
     2941 gattccaagt ggtaggcgaa cgtgaggtca acatccatga gcgttactcg attgtctgca
     3001 ttcgccgtgc tgagcggctt gtcgtttctt ctcgtcaact gcagctcgag caccccggcg
     3061 tccccagtgg gtggaacggg cggaagcact ccggcaggcg gaacttccac ggcaacggga
     3121 ggtgtgacag ccaccggcgg cgcagccacg agcggcggaa tgagctcatc gggaggtagc
     3181 aatggcggcg gggcaacaca agcgggtgcc agcagcggtg gctcgagtgt caccggcgga
     3241 gccaccgcga ccggcggaaa cagcacgagc ggcggaacga gcgccattgc cggctcgagc
     3301 agcggtggca cctctacggc gggcggcgcc ggcaacggtg gcacctctac cgtcggcggt
     3361 gcaagcggag gagcctcagc cggaacgagc gcgggcgggg ccgtgggagg aagcggcggt
     3421 gccacagggg gtacgggcgg cggcagctcc ggtggcccct tcaaagtcac cagtcccgat
     3481 cacgaagatg gtgccaagtt cggaacagct ttcacctgtg ccgccatgaa tggaatgttc
     3541 ggctccggtg tcaatccgga gttgaactgg accggagtgc ccgctggtac caagtctttc
     3601 gcgattacct tcatcgatac gaagctcggt gaaaacagcg ccatgggtca gcattgggca
     3661 gcctggaaca tcccggcgac cgccatgcag ttgccgaaag gaaccacttc gttcagcggc
     3721 cctctgatgg gtgcgaagca gacgaataag tatctctcgc cttgcccgag cggtaacgat
     3781 acttatgagt tcacggtcta tgcactcccg gtagaaagcc tctccgtcac cggggccgag
     3841 ggcaccggag tcgcgaactc cgcaggtgtg gccaaggtgc ttgcagcgtt gaagatggcc
     3901 aatccgctcg ggaatgccaa gctgagcggc acgtctggcc ccatgggtaa gtaatcgcga
     3961 gccgggctat catctggccg tacgagctag ggagggagtc gcgtgcaagc gcagagccgc
     4021 cgaggcaact cgcgcgccgt cggctctctc cagttcgcaa cgcaagcctc gaagccaaag
     4081 gccaagaagc cctagaagcc agacctggaa gaaactggag atacaaaccc agttgccgta
     4141 aatccggcag gcgtcgcata ctgcgaagcg caggggcggg attgccggtt ctccctgcaa
     4201 ggtggcaagc atgcgaacgg tgatcgggat cttgggatgg gccttggcgg tcgcgagctg
     4261 cggtgagagc acggaggctt cgccgcatgg ctcgggaggc agctctgggt cgcagcccgg
     4321 gcccaataga gcctcgaccg gaggcgcggg caacgagacg ggagacttcc ccagcggagc
     4381 gacttcgttg gcggggctgt cgtccatcgg cggcgcccat tcgaccactg ccgggagcac
     4441 cgccgcatcc agcgggggag acggtgccaa cgggtcagca tcgcctacta ggggcggagc
     4501 tcacaactcc ggcggcgcac cgagctccgg cggacgagca ccgattagcg ggggagaaaa
     4561 cgccgccgcc gaagccggcg ctgggggaga gtcgagcgcg gccggtgcgc cgcctttcga
     4621 gggcgcgcaa cgaagagtga aagagctcga cttgggaggt gcccattcgt gtgtacgact
     4681 agcggacggc aaagtcgtgt gttggggcgc taacgaagca ggccaattgg gagacggcgc
     4741 cctcggcaac cgcaatacgg ccgccccggt gcacgggcta aacaacgctg tgtccgtagg
     4801 caccggaacc gcacacactt gtgcaacgct caccgacggg acagtaacgt gttggggcct
     4861 aaacgcccac ggacaattgg gtgatcgagc accggtcaac agcatcattc ctcagtcggt
     4921 gcccggcgtt tcagccgtcg taatggtctc ggccggtgcc tggcacacgt gcgcgctgcg
     4981 agccaatggc acggtttact gttggggaga caactctcat ggccagatcg gcttaggcag
     5041 gacgagcccc gatatcgcgc cccccacgct aattccgagc ctcgagagtg tcgtgcgcct
     5101 cgctgcgagt gcagatcaga cctgtgccct gctcgagacg ggcgccatct actgttgggg
     5161 aaacaacgac tttggtcaac tcggagatgg gactcgactg ctcgatcgcg ccgcacctgt
     5221 ccccgtccaa ctcacgggac acgcgataga tgtcgcggtc ggggctcgtc acgcctgcgc
     5281 ccttctgcac gacggcagcg ttcagtgctg gggtggcaac tctgacgggg gcttgggcga
     5341 cggaaccgtg gaaccaagtc tcttgcccgt caccgtgaga ggggtgcgcg gagccactca
     5401 gctcgcactc ggagattatc actcctgcgc cctgtcgagc gacgggaaac taagatgttg
     5461 gggttacaac gcgcttggac agctgggtga tggcacgcaa accaatcgca acacggccgt
     5521 gctggttagt agcctgtccg cggtcgtgga ggtggcggcg ggcagcgtcc acacttgcgc
     5581 tctgaaccgc gatgaccagg tcgcgtgctg gggactgaac tacgacggtg aactcggcga
     5641 tgagtcgaat tcgaaccgct catcgccggt gttcgtcgta ggcatggaat gatggtcgct
     5701 tcgcctgcgc ggaaccccgt ccgttcaacg gggctcacga acacaaaacg tattgccagt
     5761 gcccgcacta gatgaactgt cgcagattcg aggtcagtca atagatgcga cggaacgcga
     5821 agttgttgta gtcggcgaac accaccccgc cattgggatg agctgcgagc ccgagtgggt
     5881 aattgacccg cgccgagacg gccggaacat actccgcgta cccaccggcg ccgtcccccg
     5941 ccgcggagaa gatcttgcct gaagataggt ctatccggcg cagcagattg gagccgccct
     6001 gcgagatgta gaggttattg gccgaatcta gcgcgatgtt cgtgaggttc acgagtgacg
     6061 ccttggtcgc caacattcct tccgtccaag ccagagtgcc tcctgcaacg tgggtgaatg
     6121 tgccgttccc cgcccgcttg accacaccga attggctatt cacgcccggg gcacacatat
     6181 aaccgctgct gatataagca ttgccgttgg aatcgaacgc aatatcgcaa ccgttcccgc
     6241 tcgagccgca agaggctaat ccggtgttcg tgccgttgca cgtaatgtac gaaacccacc
     6301 tcgatatgat ccgagttgcc ttgtccactc ggcgcagccg gtcattgttg acgtcatcga
     6361 agtagacgtt gccatcggca tccacggcaa tgtgcccggg catgtttagg ctggcggccg
     6421 tggctgggcc gccgtccccg aagttcgggc cgatcgccgt gccgccgccg gcaaacgtct
     6481 caatcgtgct gtccgccaga ttcaccactc gaacacggtg attgtaggtg tccgacacat
     6541 aaatcaggcc gttcttttca tcgagggcga gggctcccgg cgcgtaaaac ttcgcgctca
     6601 gggccggccc cgtttcgccc gcgaagccac acagatcgcc accggcgatg atctcgagat
     6661 gaccgacctt ggacaggcgg tacaccgcgc agcccgccac gaagtacagc gtgccgtcag
     6721 agccagccgc tacatcacgg gggcggctga ttcccgcgaa cggccccggg ccgggcacgc
     6781 ctgcgtagct ctccgcagta ttgatgttca cgagcgtgaa gatggtaccg ctcgtggggg
     6841 ttgacgcggt cgcgctgaac agcacggggc tgccgctcac gtgttgaccg tgaatgtctc
     6901 ggaaactggc ttcgtagcgg taggcgcctg gcgagagccc cgtccgggcc gtggcggtcg
     6961 caatgcccgt aacaccggtg gtagaagaag gcgcgtacaa accagccccg aaatcgacgg
     7021 cgcgccaagc aacattgaac cccttgagtg gcgcggcgct cccgtcgacc aactttgcgc
     7081 tgaacgcgcc gagcatcgcc cccatggtcg cagtctgcgt gtctccgctc tgcaggctca
     7141 gggtagcggt agaggggatc gtattggcgc cgcggtacac ctggcgcacg gacaagttgt
     7201 tggtgtcgac gacgtagagg tttcccgacg gatcggagct caaaccttgc ggattattga
     7261 gagcggccga cgctgctgac gcgtactcac cgccaaaggc gctcgcaccc gtgccgacga
     7321 cggtcgtgat gcggccgctc gccccctcga tgcgacgcac cttggcctca cccggttgcg
     7381 agacgtacag gttacccgcc gggtcgaacg ccaatgaatg ataactcgag agataggcga
     7441 gcgtcgccgg aacgccttcg gaggtgctgc cattcgacgc cgtattgccg gcgatgtgcg
     7501 tgagtttccc gtccggatca cgccgcagaa tacccgaata gtagctcaca cccggggcgc
     7561 atatcgttcc ggcgatgaag gcggcgccgc cgcgatccca cgccacgtca ca
//
LOCUS       DASTKU010000053         6673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_10101,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000053 DASTKU010000000
VERSION     DASTKU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10101"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="VFQ61_01735"
     CDS             <1..1548
                     /locus_tag="VFQ61_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HET9953190.1"
                     /translation="LGLGRTHAGVLQGLEAHGIRVEVSCTRGPAFFQMVGLAEAAVRE
                     ARVRVASALATLGILIDEYAITVNLAPADLRKTGATLDLAIAIAILVATGRLAARGLD
                     EMLLLGELALDGAVRPVRGVLPQLWGARQRGLSHAVVPVDNAREAGLTRSLDVRVVAH
                     LAALVHHLEGHVALEPVPETKFVPGAEPARYGDLADVRGQASARRALEVAAAGGHNLL
                     MVGPPGSGKTWLARLVPTILPPLTYDEAIECSMIHSVAGLLPNESGAILTRPFRAPHH
                     SVSDAGLVGGGESPRPGEVSLAHNGVLFLDELLEFRRSTLEALRQPLEDGQVCIARAR
                     SRAWFPARPVLVAAVNPCPCGYSGHPRRACRCSEEARRRYQSRLSGPLLDRIDLHVSV
                     PPVEVAALTSSSKAESSHAVRERVLHARARQAQRATELGLLSSLNATLAPADLERAVP
                     LDRGSRRLLEAAMNQLGLSARAYTKILRVARTVADLDGEEAVRSAHVAEAIQGRLFDR
                     EPPRSSS"
     gene            1656..2450
                     /locus_tag="VFQ61_01740"
     CDS             1656..2450
                     /locus_tag="VFQ61_01740"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HET9953191.1"
                     /translation="MTFRILTTEPVPLLEPEEPPPYRVLEGDADSPFFLTCDHASNRL
                     PKKLGDLGLAAAELERHIAWDIGAAGLSEALARELGGFLILQNYSRLAIDCNRPLSSE
                     TSITKTSEATEIPGNRGLGSEQALARIASIFEPYHRRIEHELARREQRGQSTVLLAIH
                     SFTPVFHGVARPWHVGVLYQRDTRLAHALLQLLRSNSDLVVGDNQPYSVSDTSDYGII
                     QYGERRGLPHVELEVRQDLITEPAQQTSWARRLAPMFRSALGMAGI"
     gene            2510..3484
                     /locus_tag="VFQ61_01745"
     CDS             2510..3484
                     /locus_tag="VFQ61_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953192.1"
                     /translation="MSALSWFAGEAAAQAYPRQIELTWQAPSVCPDRAQVLAAIDELT
                     GGNTETEEGGKLTVEAEVLARGDKLVLHLLWRTTNGSAERTAESVRCEDLVRVTALVV
                     ALASSPNSTAGGSSSRPLREPEATKRTKREAARAPRRSAPQSSRVRQEDSSLREKPFA
                     RGHASLLLDHGSLPRTDVGMALGVSSALGALDVGGDVVFLAPQRKEVAEGAGQFWFAG
                     VGGRACPTLKAGRISVSGCAHVELHMLRGKGQEIDFRQGGFAWFPRFGAGAELRYAVT
                     AQLSLRLIGTGLFAPWRPTFVVEAPERILVHEPDPWAERVGFGLEFRL"
     gene            3618..4139
                     /locus_tag="VFQ61_01750"
     CDS             3618..4139
                     /locus_tag="VFQ61_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9953193.1"
                     /translation="MNDLDFDSVYSAHARFVWRSLARLGVPPEHVADATQDVFVVVHR
                     RLSSFEGRSSVKTWLFGIAMRVATDWSRKVKRQPSEPLPTTLLDAASEAPFESAARSE
                     AVSTLHRLLDELTPDQRAVFVLVELEQMSVSQAAAAVGANLYTVISRLKVARQRFEAA
                     LRRHRAQEERGQP"
     gene            4136..5119
                     /locus_tag="VFQ61_01755"
     CDS             4136..5119
                     /locus_tag="VFQ61_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953194.1"
                     /translation="MSDGLGPEARALIEQALRDEGSVDSMELARIRRRVLASTLGAGV
                     IGATGEAAALKAPAALLGAGSLVKAALVGTAAAVLAFGISYVWVPPPGASKVRPVAPP
                     TAVTGARKVHATPASGPEEASSQSPSFSTTPSVIPESSVISAGGGEVPRPAERAPAVD
                     LPHSNATSGHAQDSSGVRSPRAESPPLDPLANPAARSVGQTLPAGGSAPMSRGSVLQD
                     SALAGPGMPTPAIQPPSLVDELTLLERVQNELRAGQGEQALRLLDQDRARAGDQLRAE
                     RLAAEVFASCQAGQLERARYAATRFLRQYPTTPAAARVRASCGGEHVVGRP"
     gene            5327..5614
                     /locus_tag="VFQ61_01760"
     CDS             5327..5614
                     /locus_tag="VFQ61_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953195.1"
                     /translation="MVLMLLGPALAAPKGCYFGAQEVPLGKNAIPTGAAGDATDSGGR
                     PNDEQSSTSSGGTWSGEGSRSTFGGSAGQQGAEGGETTDVVIFREGRAPVP"
     gene            complement(5677..>6673)
                     /locus_tag="VFQ61_01765"
     CDS             complement(5677..>6673)
                     /locus_tag="VFQ61_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HET9953196.1"
                     /translation="DPKLLVLVERSENALFEIHRELAEAMPNLGIKPCIADISDAARL
                     NHIFEESAPEVVFHAAAHKHVPMMEWNPGEAIKNNVFGTRLLAETAHTHGVRSFVMIS
                     TDKAVNPTSVMGATKRAAEMYIQALSQHSQTRFVTVRFGNVLGSNGSVVPIFKQQIAR
                     GGPVTVTHPDMKRYFMTIPEACQLVLQAGSMGRGGEIFILDMGEPVKIVDLARDLIQL
                     SGFSPHEIEVVFSGMRPGEKLYEELSVASENADKTRHPKIFIGRTIAEPLKDLSAKLT
                     RLEDALVNAEPTELKRALTRLVPEYSFKREAESKGPEPESDAELVVPGHQLRTVV"
BASE COUNT         1100 a         2063 c         2247 g         1263 t
ORIGIN      
        1 ctcgggctgg gtcgcaccca cgccggggtg ctccagggct tggaagcgca cggtattcgg
       61 gtggaggtga gctgcacacg gggaccagcg ttcttccaaa tggtcgggct ggcggaggca
      121 gccgtgcgag aggcgcgggt gagggtcgcg agcgcactcg cgaccctggg gatcttgatc
      181 gacgagtacg cgatcacagt gaacctcgcg cccgccgacc ttcgcaagac gggagcgacc
      241 ttggatctcg caattgcgat cgcgattctg gtcgccaccg gtcgtctcgc cgcgcgcggt
      301 ttggacgaga tgctgctgct cggtgagttg gctctggatg gtgcggtcag gccggtacga
      361 ggcgtgctgc cgcagctgtg gggagcgcgc caaagagggc tctcgcacgc cgtcgttcct
      421 gtggacaacg cccgcgaggc aggtctcacg cgcagcctgg acgtgcgcgt cgtcgcccac
      481 ttggccgcgt tggtgcacca tctcgaaggg cacgtagctc tcgagcctgt gcccgagacg
      541 aagttcgtgc ctggcgcgga gcccgcgcgc tatggggact tggccgatgt tcgcggacaa
      601 gcgagcgcac gccgcgccct ggaggttgcg gcggccggag ggcacaacct gctgatggtg
      661 ggaccgccgg ggagcgggaa gacgtggctc gcgcgactcg tgcccacgat cttgccaccc
      721 ctgacctacg atgaagcaat cgaatgcagc atgatccaca gtgtcgctgg gttgttaccg
      781 aacgagtccg gcgcgatcct gacgcgacct ttccgagcgc ctcatcactc ggtcagcgat
      841 gcaggtctgg tcggtggtgg tgaatcccca agacccggcg aggtgagctt ggcgcacaac
      901 ggggtgttat tcctggacga gctgctcgag ttcagacggt cgaccctgga agcgctccgg
      961 caaccgctcg aagatggaca ggtttgcatt gcgcgcgcgc gttctcgggc ttggtttcca
     1021 gctcgcccgg tgctggtggc agccgtcaat ccgtgtccgt gcggctactc ggggcacccg
     1081 cggcgtgcgt gccgctgcag tgaggaggct cggcgtcgtt atcagtcgcg tctctcgggc
     1141 cctctgctcg atcggattga cctgcacgta tccgtgccgc ccgtagaagt tgcagctttg
     1201 acgagcagct ccaaagccga gagctcgcat gccgtgcgcg aacgggtgct gcacgcgcgc
     1261 gcccggcaag cgcagcgtgc gacggagctc ggcttgctca gttcgctgaa cgcaaccttg
     1321 gcacctgccg atctcgagcg ggccgtgccc ttggatcgcg ggagccgccg tttgctcgag
     1381 gcggccatga atcagctcgg gctttccgcc cgagcctata cgaagatcct gagggtggcg
     1441 cggaccgtgg cggatctcga cggcgaggaa gcggtgcggt cagcccatgt cgccgaggcc
     1501 attcaggggc gtctgttcga tcgggagccg ccccgcagct cgagctgacc tccgggcgtg
     1561 cggggcaggc gcttctcacg cgcggcgagc agctttcgcg cgcacccgag cggcggccca
     1621 gccacgtcgg cttgacttta cgtccgagga tcgggatgac ttttcgcatt ctgaccacag
     1681 aacctgtgcc cctcctcgaa cccgaagaac ccccacccta ccgagtgttg gagggcgacg
     1741 cggactcgcc gttctttctg acttgcgatc atgccagcaa tcgactgccg aagaagcttg
     1801 gcgatctcgg cttggccgcc gcggagcttg aacgacatat cgcctgggac atcggggccg
     1861 ccgggctgag cgaagccctt gcccgtgagc tcggcggctt tctgattctg caaaattact
     1921 cgcgcctggc gattgactgc aaccgaccgc tcagttcgga gacttcgatc acgaaaacct
     1981 ccgaggcgac cgaaatcccg gggaaccggg gtcttgggag cgagcaggcg ctcgctcgca
     2041 tcgccagcat tttcgagcca tatcaccggc ggatcgagca cgagctcgcg cgccgtgaac
     2101 agcgagggca aagcacggtg ctgctcgcaa tccacagctt cacgccggtg tttcatggtg
     2161 tcgctcggcc ctggcacgta ggcgtcctgt atcaacgcga cactcgtctg gctcatgcct
     2221 tgctccagct gctgcgctcc aactcggact tggtggtggg cgacaaccag ccgtactccg
     2281 tcagcgatac gagtgactac ggcatcatcc aatacgggga gcggcgaggg ctcccccacg
     2341 tcgagctcga ggtgcgacag gatctcatca cggagcccgc acagcagacg tcgtgggcgc
     2401 ggcgcctcgc accgatgttt cggtcagccc tagggatggc cggcatttga gagtcaactc
     2461 tcggatgagt cgccgccgaa caggctggtc tcacgcgtgc tgtgttggtg tgagcgcgct
     2521 ctcctggttc gccggcgagg cggctgcgca agcatatccg cgacagatcg agctgacctg
     2581 gcaggcgccc tcggtatgtc cggatcgcgc ccaagtgctg gcggccatcg acgagctcac
     2641 ggggggcaac accgagactg aggaaggcgg caaactcacg gtcgaggcgg aggtgcttgc
     2701 gcggggggac aagctcgttc tgcacctgct gtggcgcacg accaatggca gcgcagagcg
     2761 tacagccgag tcggtgcgct gcgaggatct cgtgcgcgtc accgcgctcg tcgtcgcgct
     2821 ggccagtagc ccgaattcta ccgccggggg ttcgtcctcg cgcccgcttc gggaacccga
     2881 ggcaactaag cgtaccaaac gcgaagccgc gcgcgcgcca cggcgaagcg ccccccagtc
     2941 aagccgcgtc cgccaggaag actcatcgtt acgggagaag cccttcgccc gcgggcacgc
     3001 ttctctattg ttggatcacg gatcgttacc ccgaaccgac gtcggaatgg cgctcggtgt
     3061 gtccagcgcg ctgggtgcgc tcgatgttgg aggagacgtt gtcttcctcg cgccccaacg
     3121 taaggaggtg gcggagggag cgggccagtt ttggtttgcg ggcgtgggcg ggcgcgcttg
     3181 cccgacatta aaagccggca gaatctccgt ctcaggttgc gcgcacgtcg agctccacat
     3241 gctgcggggt aaggggcagg agatcgactt ccgacagggg ggcttcgcct ggtttcctcg
     3301 ttttggagcg ggtgcggagc tgcggtacgc ggtcacggcg caactgagct tgcgccttat
     3361 cggcacgggc ttgtttgctc cctggcggcc gaccttcgtg gtggaagccc ctgagcgcat
     3421 tctggtgcac gagcccgatc cgtgggcgga gcgggtgggg ttcggtctcg aatttagatt
     3481 gtgatggctc tcgtgaggat ttcgcttacc ggctcatcga ttcgctggaa ccgggtcact
     3541 aagaagacgt gacctctcgt gtgctgtccc agccggcgcc ttcggaaggg gtattttctg
     3601 cgcaatgcgt cagcgctgtg aacgaccttg atttcgactc ggtttactcg gctcatgctc
     3661 ggttcgtctg gcgctccttg gcgcggctcg gggttcctcc ggaacacgtg gccgacgcta
     3721 cgcaagacgt gttcgtggtc gtgcaccgac gcctctcctc gttcgagggc cgttcctcgg
     3781 tcaagacgtg gttgtttggg atcgccatgc gcgtcgcgac ggattggtcg cgtaaggtca
     3841 aacgccagcc ttccgagccg ctgcccacca ctctgctcga cgcagcgagt gaagccccgt
     3901 tcgaaagtgc ggcgcgttcg gaggctgtgt ctaccttaca tcggctcctc gatgagttga
     3961 ctccggacca gagagccgtt ttcgtactcg tcgagctgga acaaatgagt gtctcgcagg
     4021 ctgccgccgc cgttggcgct aacctataca ccgtgatctc gcggctcaag gtagctcggc
     4081 aacgcttcga ggcagcactg cgtcggcacc gagctcaaga ggagcggggg caaccatgag
     4141 cgacggttta ggtcccgaag ctcgcgctct catcgagcag gccctgcgtg atgagggaag
     4201 tgtcgactct atggagttgg cgcgcatccg acggcgggta ttagcgtcta ctttgggtgc
     4261 aggcgtgatc ggcgcaacgg gcgaggcggc cgcgttgaag gcgccggcgg ctcttctcgg
     4321 tgcgggctcg ctagtcaagg cagctctcgt gggcactgcg gctgcggtgc tcgcgttcgg
     4381 gatttcctat gtttgggtgc cgccccccgg cgcatccaaa gttcgacccg tcgcgccacc
     4441 gacggctgtt accggtgcgc ggaaggtcca cgctaccccg gcgagcggcc ccgaagaggc
     4501 ctcttcgcaa tccccgtcct tctctacgac gccgtcagtg atacccgaat ccagcgtgat
     4561 ttctgcgggt gggggagagg taccgcggcc agcagaacgg gcgccagcgg tagacttgcc
     4621 gcactctaac gcaacgtccg ggcacgcgca ggattcctcg ggtgttcgct cgccgcgggc
     4681 tgagagcccg ccactcgatc cgctcgccaa tcctgcggcg cgcagcgtcg ggcaaactct
     4741 acccgcaggc gggagcgcgc cgatgtcacg gggatctgtt ttgcaggatt ccgccttagc
     4801 gggccccggc atgccaaccc cagctatcca accgccgagt ttggtggacg agctgacgtt
     4861 gttggagcgt gtgcagaacg agttgcgtgc ggggcaggga gagcaggctc tgcgcctcct
     4921 ggatcaggac cgggctcgtg caggcgatca gttgcgcgcc gaacgtctag cggccgaagt
     4981 gtttgcctct tgtcaggcag ggcagctcga acgcgcccgt tacgccgcga cgcgcttctt
     5041 gcgtcagtac ccaactacgc cagcggcagc gcgagttcga gcatcttgcg ggggagaaca
     5101 tgtggtgggc aggccgtagc actgctcgag gcggtgcccc atggtgtggg cgcgtcacgc
     5161 acgagcacgt cacgagcggt cgctctgctg gcgacgctcg ctctgctggc ggagcgagct
     5221 cctgcggtgt gagtccacgc cgggctcagc gcgagatcac ttattccacc aggacattcg
     5281 aggtgtcatg acgggcacgc gactgcgcca taacggagga atgtccgtgg ttctcatgct
     5341 actggggcct gcgcttgctg cgcccaaggg ctgctacttc ggtgcacaag aggtgccctt
     5401 gggaaagaac gccatcccta ccggggccgc tggggacgca accgattccg gcggacgtcc
     5461 caacgacgaa cagagctcga cgagcagcgg cggaacgtgg agcggtgagg gctcacgctc
     5521 cacgtttgga ggcagcgcgg ggcagcaagg ggccgagggc ggcgaaacga cggatgtcgt
     5581 gatctttcgc gaaggccgcg cacccgtacc ctagtccttg acgaccgtgt tcaaggaatg
     5641 cgtcccggcg ctgctttcga agaagcagcg ttggcttcag accacggttc ggagttgatg
     5701 gcccgggact actagttccg cgtccgattc gggttcggga cctttcgatt ctgcttcgcg
     5761 tttgaagctg tactcgggga ctagacgtgt gagggcacgc ttcagttcgg taggctccgc
     5821 gttgacgaga gcatcttcca agcgtgtcaa cttagcgctc aaatccttga gtggctccgc
     5881 gatggtccga ccaatgaaga ttttggggtg acgcgtttta tctgcgtttt ccgaggcgac
     5941 cgaaagctct tcgtagagct tctcaccggg acgcatgccg gagaatacga cctcgatctc
     6001 atgcggtgag aaacccgaga gttggatgag gtcgcgagcc agatccacga tctttactgg
     6061 ctcacccatg tccaagatga aaatttcgcc gccacgcccc atggacccag cttgcagcac
     6121 gagctgacaa gcctcgggga tggtcatgaa ataacgcttc atgtccgggt gggtcaccgt
     6181 gactggtcca ccgcgtgcga tctgctgctt gaaaattggc accacgctgc cgttggaccc
     6241 cagcacgttt ccgaaacgca cggtcacgaa gcgcgtctgg ctatgctgag acagcgcttg
     6301 gatgtacatc tcggccgcgc gcttggtggc gcccatcaca ctcgtagggt tgacggcctt
     6361 gtccgtcgaa atcatcacga acgagcgcac cccgtgcgtg tgcgcggttt ccgcgagcag
     6421 gcgcgtgccg aacacgttgt tcttgatggc ctcgcccggg ttccactcca tcatcgggac
     6481 gtgcttgtgg gcggccgcgt ggaacaccac ctccggcgcg gattcctcga aaatgtggtt
     6541 gagccgggct gcatccgaga tgtctgcgat gcacggcttg ataccgaggt tgggcatggc
     6601 ctcggcgagc tcgcgatgaa tctcgaacag cgcgttctcg gagcgctcca ccaagacgag
     6661 cagtttcgga tcg
//
LOCUS       DASTKU010000054        12783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1793606, whole genome shotgun sequence.
ACCESSION   DASTKU010000054 DASTKU010000000
VERSION     DASTKU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12783
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793606"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFQ61_01770"
     CDS             complement(<1..534)
                     /locus_tag="VFQ61_01770"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9953197.1"
                     /translation="MSRLSLVIFDCDGVLVDSEPIAARIFAEALGELGLQLSHEQMFD
                     YFVGYSLRQCIDVVESLLGHAVPPGFATKLQDRTFAAFEAELRAVDGIEAALDVLPLP
                     HCVASSGSHEKMRKTLGLTGLLARFEGKLFSATQVPRGKPAPDLYLLAASQLGTPPEE
                     CVVVEDSPTGVKAGVAAG"
     gene            complement(754..2022)
                     /locus_tag="VFQ61_01775"
     CDS             complement(754..2022)
                     /locus_tag="VFQ61_01775"
                     /inference="COORDINATES: protein motif:HMM:NF014469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953198.1"
                     /translation="MGGNSGASSTTATSKPSAGSAGDSSNPHGSGGHTNAGSTSTAAG
                     GSTNTQGGSAGSQQGGTTNGSAGSSGGSSALAWDAGNPDGSCSAGLPARGQAVDTSKP
                     TTVVGSGLAASCTFAELESAVTRGGIVTFDCGPAPVTIAVTSTLRPPTNKNTVIDGGR
                     KVTLDGRQAVQIMNFTSPDFRANDNGLTLQHIRLINGKQTPTEAIPTAPAPCSQGWND
                     GEGGAIYVRDGFLTIIDSIFENNQAAPLGPDTGGGAIYVLGSKGGLTIVGSTFRNNSA
                     SNAGAVGSLFAELNIYDSLFTGNVALGHDANNNDPKTCSAINNGQNEVGSGGNGGAIY
                     SDGNSVNVTLCGDVIRDNAAGTKAFGGGLFFTSNDMGGTLTIKDSTLSGNTGGHWTNV
                     STGSVTNAGTAVGTNAKSLVIENSMLQGVP"
     gene            complement(2336..3571)
                     /locus_tag="VFQ61_01780"
     CDS             complement(2336..3571)
                     /locus_tag="VFQ61_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953199.1"
                     /translation="MNRFGALIACLVVPPFLALACGGETVPPGNPPDQGGATGVSGGK
                     TGASGGKTSASGGRTGTGNAGTPGSAGSAGTPGSAGTPGSAGGSTGSTTGPAPKGGST
                     GQHTGGTSGVKDCGLIECFRAIECVSQCGEPALTSGCCPCPEGTFDSIQCGSSGGSGG
                     GPNGGAGGASAGGTAAGGTAGKNTGAAGGEAAAGAGGTAGGASSGNVNPQRAIANPKT
                     LGTGACARKPLADVIADIHAMAPELADIDRLYDPAQMLIGDGSFIYAFATPSGFKFVL
                     VRGSGDCLAGCIDHEYWYFETDAQCSPQQVGHYSRRYSAGNCFAVQGAPLWNLPAPRT
                     DGVCPGTDLSQLNSECVDDQCPAGLTPIHFFGIAGPNGPEFCTCSIPCARDPKSCPTG
                     TSCVTISDGPGQVCSLLDR"
     gene            complement(4031..4381)
                     /gene="cutA"
                     /locus_tag="VFQ61_01785"
     CDS             complement(4031..4381)
                     /gene="cutA"
                     /locus_tag="VFQ61_01785"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HET9953200.1"
                     /translation="MSALTESALVVLCTVPDEVVAERIAHALLDGHLAACVNILPQVR
                     SIYRFEGKIEDSRELLLIVKTTRERYYALESRIKALHPYKVCEILAFDLARGSAPYLQ
                     WLVAETSSSAHPDA"
     gene            complement(4435..5190)
                     /locus_tag="VFQ61_01790"
     CDS             complement(4435..5190)
                     /locus_tag="VFQ61_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953201.1"
                     /translation="MTPTSPLTPDIETESDYQKALEAMRQRQRPAGILRVMMNALEKY
                     RQARKIGWSRPQNKYGLTVFRAYKLDACEYRELAESVFAAVSAEFSGLPAEHENFIQS
                     LKHDRELMFYVFVHDHEDRSDRYEGVTFSLGRRAANLPRHRDRLDVIIESPVQQGRSQ
                     GPSRIRMYVDPFRRPIAENPALQCGHDPLRSRHSRQLFDAGVELYASIPAADRRVWSH
                     WTRDYIDYFGPRLAPVRNSYFPTEVDEARAAVG"
     gene            complement(5187..6740)
                     /locus_tag="VFQ61_01795"
     CDS             complement(5187..6740)
                     /locus_tag="VFQ61_01795"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET9953202.1"
                     /translation="MKSLTATAKRTLKVARAARAVRAVRRAETPEQKDRARLALAEVL
                     GEARGLLMKTGQVFATNDPEDPLRQLVTGVEPLPFEVIRRAVEGELHSPLERVFGEFA
                     RRGTAASLGQVHRARLSSGELVAVKVQYPSIAAAVEAELALAGMVPGVGKVTRWGFDL
                     EGYKRELTGNMRRELDYLDEAGRQIAYRNRVCVDGLIVPRVLSEYTTRRVLVSEWVEG
                     QSIAHARALSYSERTRLGRILLETLLHSVFVAQLVHGDPHSGNFYFTRQRGPLELAVL
                     DYGCTLAIEPDAARALLALIVARRGGQQTSSLETFVRMGFDRGRLELLGGKLEAFTDL
                     LLEPFMGESACDLTQYRLGERADALLGDCKWHFRAAGPPQLFLLLRAFGGVMSQLASL
                     DVKLPFYAVLTRAVGSNRLEQAFAGSPDTLGFPARPAQSATVLTVRVRRAGRSPILLG
                     FPGCAALELRELIPDEVRDTLARDGVDLEAIERDVHQHGLTPRTLLEHAADGVLYEVA
                     LEPSRAFNS"
     gene            complement(6745..7392)
                     /locus_tag="VFQ61_01800"
     CDS             complement(6745..7392)
                     /locus_tag="VFQ61_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953203.1"
                     /translation="MTLEAFPVGIVHGVSNLEAALAFFEDVLGFPVRERGPEHAICDN
                     GSVRVKLELDLEATQPLCFELVTRDLERCTQELLDNSEVRLVKPAYWATSDRRQVVLA
                     VGDYLRLAVCRTYDEDELGLDPALPATLEWNRETDTLIRTLLRSVPSPFRVTARKRAT
                     ERAEYLTTCRGESEVHTSAAVQALIDVTPGFQHEALAEALLASGIDPTPFFGSLH"
     gene            complement(7551..8300)
                     /locus_tag="VFQ61_01805"
     CDS             complement(7551..8300)
                     /locus_tag="VFQ61_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9953204.1"
                     /translation="MKLAVMGDIHSNYAALRATLTAIEADAVDVILCLGDLVGYNAEP
                     KECLALIRARVPWVVRGNHDEDVCGDDVLLGTGRAARVVQRWTRQLLDASELAQLENL
                     PNYILQRDKFIAVHGCFLNDKHTYGYVTESMLGENLERIAARTDWPKLAFCAHTHVPL
                     LGWLDGDHVVRADLESRVVYPPQARATLLNPGSVGQPRDGDTRASYALVDIAARSVQI
                     RRVSYDIEQTIRKNQQVGLAAEFSERLREGR"
     gene            complement(8302..10314)
                     /locus_tag="VFQ61_01810"
     CDS             complement(8302..10314)
                     /locus_tag="VFQ61_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HET9953205.1"
                     /translation="MGSRRHGSGQPSRKIIVSKKLGPRRLAGSVEVHMAFVNSDTHQI
                     ILRIVYDGLANTGKTTNIRQLRGSFTPLRCSEILTPEEQDGRTLYFDFLRLDGGLIAG
                     HPVRCHLFTVPGQGRLVQRRLKLLREADAVVLVCESTEEAVTRARERLQVLREFQHVN
                     ADRSIPIVVQANKQDLPCSLSLAQIGELLGTDGSTPVLAARAQHGFGVKETLVLAVRA
                     AADLVSELIRNKGIDCIPTRGESIDGILLDLQSSEDVGEPSSFAPGSTHEASSAAREA
                     SSGSPARSSAASRPIVSLTPHKPAAAHGPRSLGSDQRPATRSDEGLSGGRLELELEFE
                     IAPRMREAPDAHPNPTPHTTIAEEQENRAVPAATPPWTGSPDLPASMPAVPIHTTPTQ
                     SAPTVAPHHPPWPSNTLPSGFIWPGTRGRELLALDYGRAVRRDDLVGQSGANDGSGRS
                     DAYVYRVADFCLKTSARRRFTDGDDARALLARTARSKVSLGELLLKDTVLALVPSDGT
                     FWLWTLTPWCQTLRSRMNAASAGEDENALGQALIEYSNAALTSLELARTRELVLDVHP
                     SNFATTEQGLVYLDDDVESGSVLMPIAHALLQRVTEYMRHPHAIAAYVGKLVEEIPLR
                     FTRRSAAALGLRSLLESASVGSRPAEQARQSLLLAVDRCPNRVSAA"
     gene            complement(10335..10739)
                     /locus_tag="VFQ61_01815"
     CDS             complement(10335..10739)
                     /locus_tag="VFQ61_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953206.1"
                     /translation="MSLDDSIQKAQRSIPECVAIGFVDMKTGMLLSAKTVDSHPQEVL
                     DLVAAATGELFQGPNVAAIERAFKKIRGVPIDDHHYFQEIVVLSDNLIHVFQRCKRHT
                     DMVLTVVSRASANLGMVLAKSRSSLPEIEAAV"
     gene            complement(10879..11286)
                     /locus_tag="VFQ61_01820"
     CDS             complement(10879..11286)
                     /locus_tag="VFQ61_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953207.1"
                     /translation="MGKIDDACRDAVQNVDGAVACGVVDLDTGMLLGIHNTAQYTQTL
                     NEIVAAASMDLFRGPGVSRIEQSVRKHRGIPEDNQHYFEEVHVSSKHNFHFMKTIKSG
                     RAVIVLITKKTTNIGMGWAQLKSVIPGVEPLVP"
     gene            complement(11288..12463)
                     /locus_tag="VFQ61_01825"
     CDS             complement(11288..12463)
                     /locus_tag="VFQ61_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9953208.1"
                     /translation="MTSVMVVDDDDALRRALARDLERDGFTVSQAESAEQALHELERS
                     DYDLVLTDLRMSQHSGLDLLRAVRANATRTATILMSAYATARDYEVALSLGSSAVLNK
                     PFSRKELNAAVHQALEAREGFRGSLHGITLLDVLQMFHFGRRSISVQLSGARSGAVHL
                     FEGEIIHAETAGLQGEIALRALLARQRGIVKTGPLQASIERSITRPFDSLVLDSLREN
                     DENGAPNPATIPPPRFSWIDAPEVEERPSGPMSVRGLDIASVRSNLPQLPPAAERALH
                     WLEATRSDTEFPWYTAAVAITLSSARTDPLAGDLGGELGPSVLEVFHSASVLTQTDAG
                     YWEAIKPMLACALIWDRQADTALLLALDRSTPRELAWFRSSAGAVARYALNHQRKET"
BASE COUNT         2339 a         4030 c         3977 g         2437 t
ORIGIN      
        1 gccagccgcg acgccggcct tgaccccggt cggactatct tcgacgacca cgcattcttc
       61 cggcggtgtt ccaagttgcg atgcggcgag taagtacaaa tccggcgctg gttttccgcg
      121 gggtacctgt gtggcactga agagcttccc ttcgaagcgc gccaacagcc ccgtcagacc
      181 gagtgtcttt ctcatctttt catgcgaccc gctcgaggcc acacaatgag gcagcggcag
      241 cacatccaag gccgcttcga tcccgtccac ggcgcgcagt tcagcctcga aggcggcaaa
      301 ggtgcggtct tgcagcttcg tcgcgaagcc cggcggaacg gcatgtccga gcaggctctc
      361 gactacgtcg atgcactgcc gcaaggaata gccgacgaag taatcgaaca tttgctcgtg
      421 cgacaactgc agcccgagct ctccgagcgc ctcggcaaag attcgcgccg cgatgggctc
      481 gctatccacg agcacgccgt cgcagtcgaa gatcacgaga gaaagtcgag acatgtttgg
      541 ttcaaaagct ccggctaacc caggatagct ttaaaccaaa cccggcggcg cgacgaggct
      601 attcgacgcg aatgggcaat ccactcaagc cactcaatct cctacgcctg tcacccccgg
      661 aaacccgcgc ccgtcgagct tcacctcgtc gagcttcacc tcgtcgagct tcaccccgtc
      721 gagcttcacg ggcgccatgg gcaattcttc ggtttagggt acgccctgca acatggagtt
      781 ctcgatcacg aggctcttcg cgttggtgcc gacggcggtg cccgcattgg tgacactgcc
      841 ggttgacacg ttcgtccagt gacccccggt attgccgctg agcgtgctgt ctttgatcgt
      901 caaggtgccg cccatgtcgt tgctggtgaa gaataggccc ccgccgaagg ctttggttcc
      961 ggcggcgttg tctcgtatca catcaccgca gagcgttaca ttgacggagt tgccgtcact
     1021 gtagatggcc ccgccgttgc ccccggagcc gacttcgttc tgtccgttgt tgatcgccga
     1081 gcaggtcttg gggtcattgt tgttggcgtc gtgcccgagc gcgacgttgc ccgtaaagag
     1141 actgtcgtag atgttcagtt cggcaaatag gctgcccact gcaccggcgt tgctggcgga
     1201 gttgttccga aacgtactcc cgacgatcgt caaaccgccc ttgctgccga gcacgtatat
     1261 cgcgcctcct cccgtgtctg ggccgagtgg cgctgcttga ttgttttcga agatcgagtc
     1321 gatgatcgtg agaaagccat cacgcacgta gatggccccg ccctccccat cgttccagcc
     1381 ttgggagcaa ggtgcggggg cggtggggat ggcttcggtg ggggtttgct tgccgttgat
     1441 gagccgaatg tgttgaagcg tcagcccgtt gtcgttggcg cggaagtccg gactcgtgaa
     1501 gttcatgatc tggacggcct ggcgcccatc caaggtcact tttcggccgc catcgatcac
     1561 cgtgttcttg ttcgtgggtg gacgcagcgt cgaggtgacc gcaatcgtaa cgggggccgg
     1621 cccgcaatcg aaggtgacga taccaccccg agtgacggcg ctttccagtt cggcgaatgt
     1681 gcagctcgcg gccaggcctg aacctaccac cgtggttggc ttggacgtat ccaccgcttg
     1741 gccgcgtgca ggcaggccgg cgctgcacga cccgtctgga tttccggcat cccaggccaa
     1801 tgccgagctg cctccggagc tccccgcgct gccattcgtg gttccaccct gctggctgcc
     1861 ggcgctgccg ccttgagtgt tcgtgctgcc tcccgccgcg gtgctcgtgg agccggcgtt
     1921 cgtatgtccg cctgagccgt gcggattgga agaatctccg gcgctgcctg cgcttggctt
     1981 ggaagtagcc gtcgtggagg aggccccaga attcccgccc atcgagttag aggttggcgg
     2041 ggagcttcca ctccctgact gcgcgacaac gggaccattc gattcattcc ccgagcaggc
     2101 tagcgagagg ctcgcgagga gcgccgctga gcagaggaac gggccagacc aagagagagc
     2161 acgagcgatg gggagcacca cacagttatg aaccgcgacc accctcaagt ggttcaggat
     2221 tttcagcgac gggcgccgct cccgatctcg cggcgctccg tacctgcggc cacgtaagcc
     2281 taaccacgta agtccagaga cgccggctgc ttacatcagc ggctcgtccg tcccgtcaac
     2341 gatcgagaag gctgcagact tggccggggc cgtcagaaat ggtgacgcag ctcgtacccg
     2401 tagggcagga cttcggatca cgcgcacaag ggatactgca tgtacaaaac tccgggccat
     2461 tcgggccggc gattccgaag aagtggattg gcgtcagtcc tgccggacat tggtcatcga
     2521 cacactccga attgagttgg ctcagatcgg tgcccggaca aactccgtcg gtgcgcgggg
     2581 cgggcaagtt ccagagcggc gcgccctgga ccgcgaagca attcccggcg gagtagcgac
     2641 gagaataatg gccgacttgt tgcgggctgc actgcgcatc agtctcgaag taccaatact
     2701 cgtgatcgat acagccggcg aggcaatccc cactccctct cacgagcacg aacttgaacc
     2761 cactcggggt ggcgaatgcg tagatgaagc tgccgtctcc gatcagcatc tgagctggat
     2821 cgtagagcct gtcgatatcg gctaattcag gcgccatggc gtggatgtcc gcgatcacgt
     2881 ctgccaaggg ttttcttgcg caagctccgg ttccaagcgt cttcgggttt gcgatcgctc
     2941 tttgcggatt cacgttgccc gaactagcgc cgccggccgt gccgccggct cccgcagctg
     3001 cctcgccacc cgccgcgccg gtgttctttc ctgccgtgcc acccgccgcc gtgccacccg
     3061 cagaagcacc tcccgcgccg ccgttggggc ctccaccgct tccgcccgaa gacccgcatt
     3121 gaatgctatc gaacgtacct tcgggacagg ggcagcagcc actggtgaga gccggctcac
     3181 cacattgcga cacacactcg atcgcacgga aacattcaat tagaccgcag tctttgactc
     3241 cgctcgttcc gcccgtgtgt tgaccggtgc ttccgccctt cggcgccggg cctgtggtgc
     3301 tgccagtgct gccgcccgca ctgcctggag tacccgcact gcctggagta cccgcactgc
     3361 cggcgctgcc cggagtgcca gcgttaccgg tgcccgtacg gcctccactt gcgctcgtct
     3421 tgcccccact cgcaccagtt ttgcctccgc ttacgccagt ggctccgcct tgatcgggag
     3481 ggttgccagg tggaactgtc tcgcccccac acgccaacgc gagaaatggc gggaccacga
     3541 ggcaggctat cagagcacca aatcgattca ttttctcacc ttcccacgca acccgactga
     3601 gacctattcg cacctatccg gtactggcga tgtaaacctc gaaggtctta tcgacggcgc
     3661 ccacaccgga gagctgccgc tacctttgca tgcacccgaa cagaaagcta cgtgagaaca
     3721 gtcgcgttcc acgcgcgcag tgcccttcgc gcgcagtcct atctgcgcag tggttctagc
     3781 tcccagcagg tggggaccct ctcaagggtt ttctgccgtt ctatccgcac tggtttattg
     3841 gcgcagtctt ttaccgagca ggttcatgca gcctctcatc ttcgaaggca ggattatcga
     3901 gggatggtag ccgggaggcg acgagtggag tcgtccgccg gccgccttgg aggagtggct
     3961 gcgtttcgtg aggtccgcct acggggggcc tgagctcggg tgccgcaggc ctgagacgag
     4021 gccgaccctg tcaggcgtca gggtgagcgg aggatgatgt ctcggccact agccactgca
     4081 ggtagggcgc cgatccgcgc gccaggtcga acgcgaggat ttcgcagact ttatagggat
     4141 ggagcgcctt aatccgcgat tcgagggcgt agtaccgctc gcgcgtggtt ttaacgatga
     4201 gcaatagctc acggctgtct tcgattttgc cttcgaagcg ataaatcgac ctgacttgcg
     4261 gcaagatgtt cacgcaagcg gccaaatgcc catccaagag ggcgtgcgcg attcgctcgg
     4321 ccaccacctc gtccggcacc gtgcacaaga ccaccagagc tgactccgtc agagctgaca
     4381 tgcgcgcgag catagcgcgc cgaagcgcta agtggtcgac gcatcgagca agcgtcagcc
     4441 cacggcggcg cgcgcctcgt cgacctcggt gggaaagtaa ctgttgcgga ccggtgcgag
     4501 gcgaggccca aaataatcga tgtaatctcg agtccagtgc gaccagactc ggcggtcggc
     4561 cgcggggata ctcgcataca gctccacgcc agcatcgaac agctgccggg agtggcgcga
     4621 gcgaagcggg tcatgaccgc attgcagcgc cgggttctcg gcaatgggtc ggcgaaatgg
     4681 atcgacgtac atgcggatcc ggcttgggcc ctggctccgc ccttgttgca ccggcgattc
     4741 gatgatcacg tccaatcgat cgcgatgtcg cggcagattc gccgcgcgac gcccgaggct
     4801 gaaggtgacg ccctcgtagc gatcgctgcg gtcttcatgg tcatgaacga acacgtagaa
     4861 catcaattca cggtcatgct tcaggctttg gatgaagttc tcgtgttctg ccggtaggcc
     4921 cgagaactcc gcactcacgg ccgcgaacac cgactccgcg agttccctgt attcgcacgc
     4981 gtcgagcttg taggctcgaa ataccgtgag cccatacttg ttctgagggc gcgaccagcc
     5041 aatcttgcga gcttggcgat atttctcgag cgcgttcatc atgaccctca aaatgccggc
     5101 cgggcgctgg cgctgcctca tggcctcgag ggctttctgg tagtcgcttt ccgtttcgat
     5161 gtcgggggtc aggggagatg ttggagtcat gagttgaaag cgcgtgaagg ttccagagca
     5221 acttcgtaca gaacgccgtc tgcggcgtgc tcgagcaacg tacgtggcgt gagcccatgc
     5281 tggtgcacgt ctcgttcgat ggcttcgagg tccacgccgt cgcgtgcgag cgtgtctcgc
     5341 acctcgtcgg ggatgagttc acggagctcg agcgccgcgc aaccaggaaa tccgagcaag
     5401 attggcgaac gccccgcgcg ccgcacgcgc acggtgagta cagtcgcgga ctgggctggg
     5461 cgcgcgggga aaccgagcgt atcagggctg cccgcgaacg cctgctccaa ccgattcgag
     5521 ccgaccgcgc gtgtgagcac cgcatagaag gggagcttca cgtccagcga agccagttgc
     5581 gacatgactc cgccaaaggc ccgcagcagc aggaacagct gcggcggccc ggcggctctg
     5641 aagtgccact tgcaatcccc cagcagcgca tccgcgcgtt cgcccaaccg atactgagtg
     5701 aggtcgcacg cgctctcgcc catgaagggc tcgagcagca agtcggtgaa tgcctcgagt
     5761 ttgcctccga gtaactcgag ccgcccgcga tcgaaaccca tgcggacgaa ggtctcgagt
     5821 gagctcgttt gctgcccccc acgccgtgcg acgatcaaag cgaggagcgc acgcgcggcg
     5881 tccggctcga tcgcaagggt gcatccgtag tccaagacgg cgagctccag ggggccgcgc
     5941 tggcgcgtga agtagaaatt gccactgtgc ggatcgccgt gtacgagctg ggcgacgaac
     6001 acggagtgca aaagcgtttc gagtaggatg cgaccgagtc gcgtgcgctc ggaataggac
     6061 aaggctcgag catgcgcgat agattggcct tcgacccact cactgaccag cactcgacgc
     6121 gtggtgtatt ccgagagcac gcgcggcacg atcaaaccgt cgacacaaac ccggttccga
     6181 taagcgatct gtcggccggc ttcgtctaga tagtcgagct cacgcctcat gttgcccgtg
     6241 agctcgcgtt tgtacccctc gagatcgaat ccccaacgcg taactttgcc cacccctggc
     6301 accatgccgg cgagggcgag ctccgcctcg acggcggctg cgatcgacgg atactgaacc
     6361 ttgactgcga cgagctcccc ggacgagagc cgagctcggt gcacctggcc taaggaggcc
     6421 gcggtcccgc gtcgggcgaa ctcgccgaac acgcgctcga ggggcgagtg cagttcacct
     6481 tccacggcgc ggcgaatgac ttcgaaaggc agcggctcca cgccagtgac gagctgccgc
     6541 agcgggtctt cggggtcgtt cgtcgcgaag acctggcccg tcttcatgag caggccgcgt
     6601 gcctccccca gcacctcggc caacgcgagc cgagcccggt ctttttgctc cggcgtttcc
     6661 gcccgcctaa ccgcgcgcac cgcgcgcgcg gcccgcgcca ctttcagagt acgtttggca
     6721 gtggcggtca gggacttcat tgcttcagtg caggctcccg aaaaacggcg tcggatcgat
     6781 gccggaggcc aacaacgctt cggccagcgc ttcgtgctga aagcctggcg taacgtcgat
     6841 cagcgcttgc actgcggcgg atgtgtgcac ctcgctctcg ccgcgacacg tcgttagata
     6901 ctccgctctc tccgtagccc gcttgcgtgc cgtcactcgg aacgggctcg gcacgctgcg
     6961 gagcaaggtg cgaatcaacg tatcggtttc gcggttccat tcgagagtgg cgggcagagc
     7021 cgggtccaga ccgagctcgt cttcatcgta agtacggcac accgcgagtc tcagataatc
     7081 tccgaccgcg agtaccacct gacggcggtc actcgttgcc caataggctg gtttcaccag
     7141 ccgaacttcg gagttgtcga gcaactcctg cgtgcacctc tcgagatccc tagtgaccag
     7201 ctcgaagcac aggggctgag tcgcctccag atccagctcc aatttcactc ggacagagcc
     7261 gttgtcgcat attgcgtgtt cggggccgcg ctcgcgtacg ggaaacccca aaacgtcctc
     7321 gaagaaggcc agcgcagcct ccaaattgga gaccccgtgt acgatgccta ctggaaaagc
     7381 ttctagcgtc attgtctctc aatcgggtgg ggcgtgtgcc tttggcgtcc cgcgcagaaa
     7441 ttcggcaaag ttgcgaatgg gttgagccgt gctcggctta cgacgtgatc aggccgcaac
     7501 gcgctaatgc agggtagcgt ctgccgtgat tggttcggtg ttggatacgt ttatcgtccc
     7561 tcccgcagcc gctcactgaa ctccgcggcc aatccgactt gctggttctt ccgaatcgtc
     7621 tgctcgatat cgtagctgac tcggcgaatc tgcacgctcc gtgccgcgat atcgaccaag
     7681 gcgtagctcg cacgggtatc gccgtctctc ggttgcccca ctgagccggg gttcaatagc
     7741 gtggcccgag cctggggcgg atataccacc cggctttcca gatccgctcg aaccacgtga
     7801 tctccgtcga gccaacccag cagcggcaca tgggtgtgag cgcagaaggc gagcttgggc
     7861 caatcggtgc gtgccgcgat tcgctcgagg ttctcaccca acatggactc cgtgacgtag
     7921 ccgtaggtat gcttgtcgtt caagaagcaa ccgtgtacgg caatgaactt atcgcgttgc
     7981 aagatgtagt tcggcaggtt ctccagctgc gcgagttcgc tcgcgtcgag tagttggcgc
     8041 gtccagcgtt gcacgacgcg cgcggcgcga ccggtaccga gcaaaacgtc atctccacac
     8101 acgtcctcgt catggttgcc gcggaccacc cacggcaccc gcgcacgaat cagggctagg
     8161 cactccttcg gttcggcgtt gtacccaacc aagtctccaa ggcaaaggat gacgtccacc
     8221 gcgtccgcct caatcgcggt gagggtggct cgcagcgcgg cgtagttcga atggatatcc
     8281 cccatgacgg cgagcttcat gttaggcagc gctgacgcgg ttggggcagc gatccacggc
     8341 tagaaggagg ctctggcgcg cctgttcggc gggccgtgag ccaacactgg cggactcgag
     8401 cagtgagcgt aagcccagcg cggccgcgct gcgtctggtg aaacgcaaag ggatctcctc
     8461 gaccagcttg cctacgtagg ctgcgatggc atgcggatgt cgcatgtact ccgttactcg
     8521 ttgcaggagc gcgtgtgcga tcggcatcaa aaccgagcca ctctcgacat cgtcgtccag
     8581 gtacactagg ccctgttcgg tggtcgcgaa gttggaggga tgcacgtcga gcaccagctc
     8641 tcgagtccgc gcgagctcca aagaagtcag ggctgcattg gagtattcga tcagggcttg
     8701 cccgagggcg ttctcgtctt cgcccgcgga ggcggcgttc atgcgcgaac gcaaggtttg
     8761 gcaccaaggc gtcagcgtcc aaagccagaa cgtaccgtca ctgggtacga gcgccagcac
     8821 ggtgtctttc aagagcagtt caccgagtga aaccttgctg cgtgccgtgc gggcgagtag
     8881 cgcgcgggcg tcatcaccgt ccgtgaagcg ccggcgtgcg gaggttttga ggcaaaaatc
     8941 cgcgactcgg taaacatagg catcgctgcg tccgctaccg tcgttcgcgc ctgattgtcc
     9001 cacgagatca tcgcgtcgca ccgctcgtcc gtagtccagg gccaagagct cgcggccgcg
     9061 cgtgcctggc caaatgaagc cacttggcaa cgtgttcgag ggccaagggg gatggtgcgg
     9121 agcgacggtc ggcgcagact gggtcggagt cgtgtggatt ggtacagcgg gcatcgacgc
     9181 cgggagatcg ggagaccccg tccacggggg agttgccgca ggcacagccc ggttttcctg
     9241 ctcttcagcg atggttgtgt ggggagttgg attggggtgc gcatccggag cttcacgcat
     9301 ccgcggggcg atttcgaact ccagttccag ttccaacctg ccgccgctca gtccttcgtc
     9361 cgaacgagtg gctggccgct gatcggaccc gagcgaacga ggtccgtgtg ctgcagcggg
     9421 cttgtgaggc gtgagcgaca cgatcggccg cgaggctgcg ctgctgcgag caggggaccc
     9481 actcgacgct tcacgcgcag ccgacgacgc ttcgtgtgtt gagccgggcg caaacgacga
     9541 gggctcccca acatcttcgc tcgactgcaa atcgagcagg atgccgtcga tgctctcgcc
     9601 gcgcgtcgga atgcaatcga tgcccttgtt ccggatcaac tccgagacca aatcggccgc
     9661 cgcacgaacg gcgagcacga gtgtctcttt gaccccgaag ccatgttggg cgcgcgccgc
     9721 caacactgga gtgctgccgt ccgtgcccag caattctccg atctgcgcga gcgagagcga
     9781 acacggcagg tcctgcttat tcgcttgcac gacgattgga atggaccgat cggcgttcac
     9841 gtgctgaaac tctcgcaaga cttggagccg ttcgcgcgct ctggttaccg cctcctccgt
     9901 actctcacac accaacacga ccgcgtccgc ttcccgcaga agcttgaggc ggcgttgtac
     9961 gagacgaccc tgtcccggta cggtaaacag gtggcagcgc accggatgtc ccgcgatcaa
    10021 accgccgtcc aagcgcaaga agtcgaagta cagggttcgg ccgtcctgct cttcaggcgt
    10081 gaggatttcg ctgcagcgca gcggcgtgaa cgagccgcgg agctgacgga tgttggtggt
    10141 cttgccagta ttggcgaggc cgtcgtagac gatcctgagg atgatctggt gtgtgtcgct
    10201 gtttacgaaa gccatgtgaa cttcgacgct gcccgcgagg cgccgcggcc cgagcttctt
    10261 ggatacgatg atcttccgtg acggctggcc tgagccgtgg cgcctgctgc ccactcctcg
    10321 tgacggctcc gctctcagac cgcggcttcg atttctggca gagaagagcg tgacttcgcg
    10381 agcaccatgc ccaggttggc ggaggctcgg ctcacgacgg tgagcaccat atcggtgtga
    10441 cgcttgcagc gctggaacac gtgaatcaag ttgtcggaca gcacgacaat ctcctggaag
    10501 tagtgatgat cgtcgatcgg tacgccacgg atcttcttga aggcgcgctc gatggccgcg
    10561 acgtttgggc cttgaaacag ctcccccgtg gccgctgcca ccaaatcgag cacttcctgc
    10621 gggtgactgt cgacggtctt ggccgacaac agcatccctg tcttcatgtc tacgaaaccg
    10681 attgcgacgc actccgggat cgagcgttgc gccttttgga tcgaatcgtc gaggctcatg
    10741 aggtttcttt ctcgctgggt gttacgggtt ctcgtgacac ggcgaaacgc agcgccgctt
    10801 ccgccgggag ccgcgctcgc tcgggccgag gcgccatggc gcctgcagcc gctaagaagg
    10861 agccgaatgg gctaagcctc aggggaccaa gggctccaca ccggggatga ccgatttgag
    10921 ttgagcccag cccatcccga tattggtggt cttcttggta atcagtacga tcacggctcg
    10981 tccgctcttg atggtcttca tgaaatggaa attgtgtttc gagctgacgt gtacttcctc
    11041 gaagtagtgc tggttgtctt cgggaattcc ccggtgtttg cgcactgatt gctcgatgcg
    11101 cgagacaccc gggccgcgga acaagtccat ggacgccgcc gcgacgatct cgttcagagt
    11161 ctgagtatac tgtgccgtgt tgtgaatgcc gagcaacatg cccgtatcta gatcgacgac
    11221 cccacaggcc acggcaccat ccacgttttg aactgcatct ctgcacgcat cgtcaatttt
    11281 tcccatttca ggtctccttc cgctgatgat tcaaggcgta acgcgccacc gctccggcgc
    11341 tcgagcgaaa ccaagcgagc tcacgcggcg tcgagcgatc caacgccaac agcaaggccg
    11401 tatccgcctg ccgatcccag atcagcgcac acgccaacat cggcttgatc gcttcccagt
    11461 aaccggcgtc ggtttgagtc aaaacgctcg cggagtgaaa cacctccaag acggagggcc
    11521 cgagttctcc gcccaggtcg ccggcgagcg gatccgtacg cgcgctactc agagtgatgg
    11581 ccaccgcggc ggtgtaccaa ggaaactcgg tatcggagcg agttgcctcc agccagtgca
    11641 acgcacgttc cgcggcgggt ggcagctggg gcaggttgct gcgcacgctc gctatatcca
    11701 agccgcgtac ggacatcggc cccgaggggc gctcctccac ttcgggggcg tcgatccacg
    11761 agaagcgcgg gggcgggatg gtggcggggt tcggggctcc gttctcgtcg ttctcgcgta
    11821 gtgaatcgag caccaggcta tcgaagggcc gggtgatact gcgctcgatc gaggcctgga
    11881 gtgggccggt cttcacgatc ccgcgctgtc gagccaatag agcacgcagc gcgatttctc
    11941 cctgcaaccc cgccgtctcg gcgtgaataa tctcaccttc gaagaggtgc acggcgccac
    12001 tccgcgcgcc ggagagttgc acggagatcg agcgccgccc aaagtgaaac atctgcagca
    12061 cgtcgagcaa cgtgatgccg tggagcgagc cgcgaaaccc ttcgcgcgcc tcgagcgctt
    12121 ggtgcacggc ggcgttcaac tccttacgag agaagggttt gttgagaacg gcggagctgc
    12181 ccaacgagag cgcaacttcg taatcgcgcg ccgtcgcata agcgctcatc aggatcgtgg
    12241 cggtgcgggt ggcattggcg cgaacggccc ggagcagatc gaggccgcta tgctgcgaca
    12301 tccgcagatc cgtgaggacc agatcgtagt cggaccgctc tagttcgtgc agggcctgtt
    12361 cggccgattc agcctgactg acggtgaatc cgtcacgttc gagatcacgg gccaaggctc
    12421 gacgaagcgc gtcgtcatcg tccaccacca tgacgctggt catgttggct caccgcgagg
    12481 gcgtacgtgc gcgaccccca ggagtggagt gcttgtgccg agacagcgca gatctagctt
    12541 cggccgcgtt tccaatcggg ccttcgagtt cgaacacacc tccagcacgt aggtcctctc
    12601 ctgggcggcg atgccgcgaa ccggtaacaa acaaccgcac cgacgctccc gctgtgagcg
    12661 cgagcaacgc acttcggcgc gcacctcgag gctccattca gcaacgccca tgccacgcgt
    12721 gcgatcacta gattcgtccc aaaaacgagc tctgaaacga gcaggtgcgc tcgtgtgcgg
    12781 cgc
//
LOCUS       DASTKU010000055        19808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1793745, whole genome shotgun sequence.
ACCESSION   DASTKU010000055 DASTKU010000000
VERSION     DASTKU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 19808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19808
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793745"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="VFQ61_01830"
     CDS             complement(<1..1174)
                     /locus_tag="VFQ61_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HET9953209.1"
                     /translation="MTTTTTLDLACPVTHVTVFEDRARVTRSGLVALGAGTTRLRVVG
                     VSPVLVDRSLCARVQPNSELARAARVSHARVVRERVVLRDAEPEKLRELTQRLEQLEL
                     DRAEFLQRQKELVAHIEQLSLLESRTLREFAEDVSFGQTVPEAAAAQIADLAARKRAA
                     LCRAVEVEEQGADLEQALQDLQGRLHAAQDPSAELRADLVMDVLAEAETSAELVIDYT
                     VPNAAFRPQHTAKLDERKLTFTSDACVWQNTGEDWQDIQLRLSTERPSLGLEPPLLET
                     DLLLARRTSAVISVEARDEKIHMAGLGASQSSDTLPGIDDGGEPIEFVAPAKATIPSD
                     GRPYRVIVQRFEAAVGLGLMCRPELAQRVFRRAVSENASRRPLLAGPVDLIECDGLV"
     gene            complement(1200..3407)
                     /locus_tag="VFQ61_01835"
     CDS             complement(1200..3407)
                     /locus_tag="VFQ61_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HET9953210.1"
                     /translation="MSDVMGSAERVSRIDAVTVYRQGARVTRSLLVERTEQGYPEQVI
                     FDALPMCLEDGSLTVRVTPLGASPAELPVASALKVALAVPAANPELRPAESAALRAAR
                     LQLKKQTELYDQCQREVTALAALQMLDRGRTKQGPPPPSPVAQRLGLLAFRTARAEEL
                     ARSTREAAAQLQLAKKQLARLEDEEARRTSARNLRPDELAKRVIVELTQHHAATPRVR
                     VSIEYHVRGARWAPAYTLSLDAQGRAELGLRALLRQRTGEDWKGVALQLSTADATRQT
                     ALPELPQLLIGRAQRPARRGFRPLPDGLESLFEDYDRARAAAFPAAATEFPNSRQGLA
                     VAAAGGAHAAAAHAPPPPARPPAAPPAPVAAPAPAPAPEAFRMLAAQRELAEPQPVRR
                     RRTMSAEPPRLSSMAGESAPPASLGAAPFGAPGALPDAQAGADLLQPEPELSLSDAQL
                     DYGSLRMPGPLTPQRGRLGRASRLELYAEYTHVDVVVARVQFEQVALSVKRARELESS
                     APPSGYVYPNDDVFPALYAAEQPVDVTSDDEFHTVEVCRCAMQAALEYVAVPREAPHV
                     FRQVTGVNPLPGPLLEGPIDVYMDGQYAMSSRLRTTPPGGKIQLGLGVEQRIKVARNT
                     TYTEETGGLLRGHLDLLHGITIDLQSHLNVDVRVQIRERIPVTRPKEEELRVTLQTVE
                     PAWRAYEEQTPPIEGAYAWHITLPASGKTSLKVRYAVRVPSKSELVGGNRREG"
     gene            complement(3879..4589)
                     /locus_tag="VFQ61_01840"
     CDS             complement(3879..4589)
                     /locus_tag="VFQ61_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9953211.1"
                     /translation="MHTAGRRGATRVVSFELMRTPPPTPDDPTVYPVEEPVGEEILQC
                     WMLEVLRPLIERWLAERGTPCFVGSDQFIYFEKGQVSQRIAPDIYVLPDVPPDSDIPS
                     WKIWESGIHPSFALEICSQDWRKDYETAPRLHHQIGTEELIVVDPDYLKRREGYRLQR
                     FERTSRGLELQERTQADRVRSRVLGCFVRLVGTERPRQRVRLALDPNGDILFPTDAER
                     AEAALARVAELEALLKHS"
     gene            complement(4823..5668)
                     /locus_tag="VFQ61_01845"
     CDS             complement(4823..5668)
                     /locus_tag="VFQ61_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="expansin-like protein"
                     /protein_id="HET9953212.1"
                     /translation="MYWSRWCWLGGMLGVVACVPAGSNDVSEPVNTAAGARAGVPSRC
                     VGNCDAATPRPPEVVDNGGAAFGNVTMYSTSPSNGGACLYGATAIESFAAIHVNLEPG
                     DARGQWDAGRICGQCARVTVITSQGPKQVVVRIMDKCPDSFCGLDLGGTAPAAVMLDG
                     FGRYDGGWEWISCVGHSQVFDGPTALFVKEGSNAYWAAVQVRNPPMSVASVQWLNLTE
                     PTRRGGFEYAGPQIENYYLVPTELLRSPSRFQVTVTYRDGSTSHLELSGAQLAVAEAS
                     YPFPL"
     gene            complement(6532..8019)
                     /locus_tag="VFQ61_01850"
     CDS             complement(6532..8019)
                     /locus_tag="VFQ61_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953213.1"
                     /translation="MNRELAAAMSCVRVGRARALFLTCAIMTASWGCERSHTRDSSAS
                     SRKDVTPRAPRFWLTPGPALPGATRGVLSAPLQHSVLVELLPTGRIRTPDSKDLAAEA
                     ELPRDSQSKAEGRGLALYVQERADLHYGAPDGPVFGRLHPGALVRVAVAGEGSFLSNS
                     SRVRVGSIGPSFGAGQTAAFCDRKVLSTKEVPLSEYQPEPGSEALRSGFNVWGTIPNQ
                     SETLYNVSFSPCYDTFVLRKQHLLRQYIEGVETFGEDIDHNLDSDSSIVARSVSWCTG
                     RVVTQGPGTLLLHRPGFEPQGVAQIPAGYEVLTRPQTNVEPLSSRVRAGRGELYWIVA
                     TEGKLFCSGWRFKKFTGPRENRDDVPLPKDLVAPVLTGKLESQEAMDGKGLSYPIEYV
                     PPTPHQAAQLWFGSLRWGGNRYLNCSSCESNYTVLKTLKDELWVSNRVLPDDVLAFDP
                     NEKERWFFSRVACEAALAGARAELQRSPTSGTRLGIHARSNIVPH"
     gene            complement(8551..9303)
                     /locus_tag="VFQ61_01855"
     CDS             complement(8551..9303)
                     /locus_tag="VFQ61_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9953214.1"
                     /translation="MHTAGRRGATRVVSFELMRTPPPTPDDPTVYPVEEPVGEEILQC
                     WMLEVLRPLIERWLAERGTPCFVGSDQFIYFEKGQVSQRIAPDIYVLPDVPPDSDIPS
                     WKIWERGIHPSFALEICSQDWRKDYEVAPRLHHQIGTEELIVVDPDYLKRREGYRLQR
                     FERTSRGLELQERTQADRVRSRVLGCFVRLVGTERPRQRVRLALDPNGDILFPTDTER
                     AEEQRQRADAEKQRAQAALARIAELEALLEGR"
     gene            9784..11592
                     /locus_tag="VFQ61_01860"
     CDS             9784..11592
                     /locus_tag="VFQ61_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953215.1"
                     /translation="MGPTRAVPSNDSLGELIGFRVADGLRYAATGAKTRVVSAYDLEL
                     HAGATARAMRSIHAWLRLPVEQAFERILGERPPPLSAYVQPTVIRAENSLVIDLDLYC
                     VETKAEKSPAIISKRKSVRGSVDNQSALLEQAFTAASALFQEGEPRRPVARVAGGIQQ
                     RFPVGDPVVLDAGLSSDPDSDALVFEWTGPAEIGWRQEGARVTLSPSRPGKYAFMVKV
                     SKLLDRSSSESLVVAVQVVAPPVIRVQGGSMVAMNGRTKVVLDGTCEGCEDWRWTQAS
                     GPVTTLPSTCGPDRSAEMRGPRVPTEPGSDNMTSARCEVWADAPGQYVFTLEGVNAIG
                     SASKDVTFGVAPAPVALFDAPDVAVVGHSYSLAGTASHDVFGGNLRYHWEVADQEFPK
                     QTCAPINRTLPAGVSLSTPTQAVTEFSAARPGKYYVRLTVLANRTFGGHASANQECRQ
                     QAILVEKPIWWFTIEAGGDFLPEGDINRYWEGFRLKGAVTVRPAFARKVPWIGPSLGL
                     RVAQSFIQSNDQGKDFRFGGGTSLGLALLFSDSIQFHASAFARTWGKTSVGPEMGLEF
                     RVQRMLVLSADVRAERRDNEWHVPVVGGFFGAGYDL"
     gene            11589..12662
                     /locus_tag="VFQ61_01865"
     CDS             11589..12662
                     /locus_tag="VFQ61_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953216.1"
                     /translation="MKHPARALCFLAAAALFAPGAHADPLLVEGPSLPTAELEWIRGQ
                     LVNGSFGGASQSLPPGVSVTVKLTVEQGQWCARGSLSAWDEPTPEELEKRCSAMGTSL
                     LGTQRYAPSHAALSRFIIEPLASDITRRLSSRWQLRIAHTTPESLNISVGDRDAKPYL
                     AMSETGVATLSVKSGSKVTVSAGSCTRILDAATPIIDLEQLRTVRTFSPVWTREGQAI
                     QSLTLDTGDVTRLTLRLDEESNQGAIALPCSGAISARIMGETFSATSIETKPESWTWS
                     IEAKKSPGIIHVGLRAAEGQSDKETAISLSFEPPWWTRLWVKVSGALLGAGALLKGIL
                     EIKKSIQDLRGKKNSPTEKAPQE"
     gene            12980..13822
                     /locus_tag="VFQ61_01870"
     CDS             12980..13822
                     /locus_tag="VFQ61_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9953217.1"
                     /translation="MQPPPSVRDPHEPEVPPAEPALASASSAPAGSGSVLRGGLVALA
                     IGVVAKGKGLWIGLKALPLAKVFLTGGSMIASVLAYAIHGGFAFGVGLVLMILIHELG
                     HGFAMKRAGVDSGWPVFIPFFGAMIAMKGKPEHPRAEAAIAYGGPLAGTAAALSCAAL
                     GLVLRSPFFLALAYTGFFLNLFNLAPFGFLDGGRIARVISRNAWIAGAIILGGMCLMS
                     PSPQLILIAVLGAAQAFRRDNTDLERVTDDDRRAWAFRYFGLCGFLGLAVVFSHQLTR
                     AHGW"
     gene            13952..15520
                     /locus_tag="VFQ61_01875"
     CDS             13952..15520
                     /locus_tag="VFQ61_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953218.1"
                     /translation="MAACGKRRFALFGGLLLACGAPSGGAPSGGRELTREEIPVLEPG
                     WDIPIVDESALPEPAPEAGFIALEKTAYQARLEGDREFTSEPARLFYNFVPAETGAKE
                     KPVIVLFNGGPGAPATLLRCFGTGPNSLDAEDLSAPPAPNPYRFTRFGSLLYIDPRFS
                     GFSYGVTEHPESDAEREQAFREDSLNPTRDAADFAQVILRVLNKIPAIRNNPVVLVGE
                     SYGGTRAALMLDILTDLGDDFSIYVNQAWKAELDEHFASVFARPERANRASRAKQFGW
                     QILIQPLLFGAAQYDKMGDFSQRLYRDPAGPCRYHVGRTGAWCEELERAATRTMVDPK
                     SFETIVGVSPQRVPGLAASDRDGAFRGARSEATSETGFEELLGGLPTWDAYYTDQISV
                     ALGNLTRTTEIYSYVHTALRSLTQTQTLITNALMDGVIESSSILEVLASMDGIQIESV
                     AMPSTAQADTISPADRPVDRLYTVRYPAADQTREFTLFFPVYPDSGHAVTVSQPDKFF
                     ADVQHFLASTGLGR"
     gene            complement(15874..18576)
                     /locus_tag="VFQ61_01880"
     CDS             complement(15874..18576)
                     /locus_tag="VFQ61_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET9953219.1"
                     /translation="MNRSLHSPSRFLLLGCLIGCNAASSQPDVGAGASGAAGHQGGAQ
                     PHSGGATQGQNERGGSHSGSDDRGGTQSGGTHAEGGMTGGEGGAGTDGPSSGGITSTG
                     GSSGESEPVTGGSADGGAQGGGSHLGGSAHGGGDAQGGSHLGGSAGEDAGAGESGGGS
                     DAGSSGSAGAAGMPSGEAPKCEGTLGLPGLPSVPVARSPRYVVSGDWDGDRRADLAVV
                     GAVANSVGVLLSQGAGKFAPITEYSVPLQPTSAVAGDVDGDGALDLVIVTSDPSQSLV
                     FLINLGDGKFELDSTYTAELTVLPRAVTISDLNGDGRADLAVTGSGGLNVLLGQGGGR
                     FRLMASSADPWSAGAIGAADFNDDGRVDLALIPSYGTELKLFVGRGDGTFEAPSSHFT
                     GIPDQDPPALSSTSLSVADLNGDGASDIALASEPASEIAVLLGRKDGTFASATHYEAP
                     SGPHSLVAADLDGDRKPELAVVSPYVNVTAVLRNLGDGTFASRREYAGGAQTWALAVA
                     DLDGDGSADLAVVNESTDDLSVLINDQHGSFAAPHSLNPTRSFDPVRYPDSVAAADFN
                     HDGRSDLAVINANTQKMSVFTGDGGGAFAPAENHTTQTTAKRLIAADLNGDGWDDVVL
                     AGDGCEVLLNDGTGRLNHSDSYVAGQRTESVIAADLNGDGAPDLAAVNFGSNDVSVFV
                     NRGDGRFAAAKNFRSGVAPTALTATDLNGDGHTDIIVSNSGAFIPGSGFPANPPRETV
                     SVLFNVGEGGLSSPVTYALPAAPGAITHGDWNGDGKPDVAVASYTGTDIVGQIYILLN
                     TGDGILGAPVSYPVGRVPTGLARLDLNNDSRADLAVANSQGNITVLLNQGAGRFNTSH
                     YAAGRGPTSLAAADFDRDGRLDLAVTNPNDNNNDVRILLNRCLE"
     gene            18785..19582
                     /locus_tag="VFQ61_01885"
     CDS             18785..19582
                     /locus_tag="VFQ61_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9953220.1"
                     /translation="MMNSVPGARNFVATGETYDLFMGRYSRPLAVLFADAADLAPGMK
                     ALDVGCGPGALTGVLVDRLGAANVAAIDPSPTFVEACKARHREVDVRSGRAETIPFED
                     QSFDRVLAQLVLHFVSEPARAAQELRRVLRPGGMALACVWDFTAGMEMLRHFWDAALA
                     LNPEAPDEARILRFGREREILDLFAATGFENLTETTLQVTSSYIDFDELWSGFLAGIG
                     PAGAYCMSLSEEERATLRAELFSRLGQPRGAFSLGAMARCASGRAPA"
BASE COUNT         3498 a         6370 c         6284 g         3656 t
ORIGIN      
        1 ccaccaaacc atcacattcg atcaagtcta cagggcccgc caagagtggc cggcgcgaag
       61 cgttctcgct cacggcgcgc cgaaagaccc gctgagctag ctcggggcgg cacatcaaac
      121 ccagccccac ggcggcctcg aagcgctgca cgatcacgcg gtagggtcgg ccatcgctcg
      181 ggatagtggc cttggcggga gccacgaact cgatcggctc ccccccgtcg tcgataccgg
      241 gtaaggtatc gctgctctgg ctggcgccca gcccggccat atgaatcttc tcgtcccgcg
      301 cctcgacgga gatcacggcg gaggtccggc gagcgagcag caagtccgtt tcgagcagcg
      361 gcggctccag acccaagctc gggcgttcgg tggagaggcg cagctgaata tcctgccaat
      421 cctcgccggt gttttgccag acgcaggcgt ccgaggtgaa ggtcagcttg cgctcgtcga
      481 gctttgccgt gtgttgcggt cgaaatgcgg cgttcggcac tgtgtagtcg atcacgagct
      541 cagcgctcgt ctctgcctcc gccagcacgt ccatcacgag atcggcgcgc agctcagcgc
      601 tcgggtcctg agccgcgtga aggcggccct ggagatcctg cagcgcctgc tcgagatcgg
      661 cgccctgctc ctcgacctcc accgcgcggc acagcgcggc gcgcttgcgg gcagcgaggt
      721 cggcaatctg agcggccgcc gcctcgggca cggtctgacc gaaggacacg tcttcggcga
      781 actcccgcag ggtccgcgac tccaggaggc tcagctgctc gatgtgagcc acgagctcct
      841 tttggcgctg caggaactcg gcgcggtcga gctccagttg ctccaacctt tgcgtcagct
      901 cgcgcagctt ttccggctcc gcatcgcgga gcacgacgcg ctcccgcacc acgcgcgcgt
      961 ggctcacgcg cgccgcgcgc gctagctccg agttcggctg gacccgcgcg cacagggagc
     1021 gatccaccag cacgggcgat acaccgacca cgcgtaggcg cgtcgtcccc gcgcccagcg
     1081 cgacgaggcc gctgcgcgtc acccgagcgc ggtcctcgaa gaccgtgaca tgagtcaccg
     1141 gacaggccaa atccagagtg gtggtcgtgg tcatgggttc tcggctcatc cttcccggct
     1201 catccttccc ggcgattgcc ccccacgagc tcgctcttgc tcggaacacg caccgcatag
     1261 cggaccttca ggctggtctt gcccgaggcc ggcagcgtga tgtgccaagc gtaggctcct
     1321 tcgatcggcg gagtctgctc ttcgtaggcg cgccaggcgg gctcgaccgt ctgcaacgtc
     1381 acgcgcagct cctcttcctt ggggcgggtt accgggatgc gttcgcggat ctgcacccgg
     1441 acatcgacgt tcaggtggct ttgcagatcg atcgtgatgc cgtggagcaa atcgaggtgt
     1501 ccgcgcaaga gcccgcccgt ctcctcggtg tacgtggtgt tgcgggcgac cttgatgcgc
     1561 tgttcgacgc ccaaaccgag ctggatcttc ccgcctggag gcgtcgtgcg cagccgcgag
     1621 ctcatcgcgt attgaccgtc catgtagaca tcgataggcc cttccaacag cggcccgggt
     1681 agcgggttta cgcccgtgac ctgacggaaa acgtgtgggg cctcccgcgg cacggccaca
     1741 tactcgagcg cagcttgcat cgcgcagcga cacacctcga ctgtatgaaa ctcgtcgtcg
     1801 gacgtcacat ccacgggttg ctcggccgcg tacaacgcgg gaaagacgtc gtcgttcggg
     1861 taaacatatc cggagggtgg agcgctggac tcgagctctc gggcgcgctt cacggacagc
     1921 gccacctgtt cgaactggac gcgcgcgact acgacgtcca cgtgggtgta ctcggcatag
     1981 agctcgagcc tcgatgcccg gccgaggcgc cctcgctgcg gcgtgagcgg gccgggcatg
     2041 cgcaggctgc cgtagtcgag ctgcgcgtca ctgagcgaga gctcgggttc gggctgtaag
     2101 aggtccgctc ccgcttgcgc gtccgggagg gctccgggcg cgccgaacgg agcggctccg
     2161 agcgaggcgg ggggagctga ctcacccgcc atcgagctga gacgcggcgg ctcggctgac
     2221 atcgttctgc gccgccgcac tggctgcggt tccgcgagct ctctctgggc cgcaagcatc
     2281 ctgaaagctt cgggcgcagg cgccggtgca ggcgccgcca cgggtgcggg gggcgcggcg
     2341 gggggacgtg cgggaggcgg aggcgcatgg gccgccgccg cgtgagcgcc acccgccgct
     2401 gcgactgcca gcccttgccg tgagttgggg aactccgtgg cagcggccgg gaaggcggct
     2461 gcgcgcgccc gatcgtagtc ttcgaacagg ctctctaggc cgtccggcaa gggtcggaag
     2521 ccgcgcctcg cggggcgctg ggctcggcca atcaagagct gaggcagctc gggaagagcc
     2581 gtctgccgcg tcgcgtccgc cgtcgagagc tgtagggcga cgcctttcca gtcttcgccc
     2641 gtgcgctgac gcagcagcgc gcgcaacccg agctcggctc gaccttgcgc gtcgagcgac
     2701 agcgtgtaag cgggggccca acgcgcgccc cgcacgtggt attcgatcga cacgcgcacg
     2761 cgcggggtgg ccgcgtggtg ctgggtcagc tcgacgatca cgcgcttggc caactcgtct
     2821 ggacgtagat tgcgagcgct ggtccgacgc gcctcctcgt cttccaatcg ggccagctgc
     2881 ttcttcgcca gctgcagctg cgctgcggct tcgcgcgtac tgcgtgccaa ctcttcggcg
     2941 cgcgcggtgc ggaacgcgag cagtccgagc cgttgcgcca ctggactcgg cggcggaggc
     3001 ccttgcttcg tgcggccacg atcgagcatc tgcaacgccg ccagcgcggt cacctcgcgc
     3061 tggcactggt catagagctc cgtctgcttc ttgagctgga ggcgtgccgc tcgtagcgcc
     3121 gcggactcag cgggtcgcag ctcggggttc gccgcgggca cggcgagcgc gaccttcaac
     3181 gcggaagcaa ccggcagctc tgcgggcgac gcgcccagcg gcgtcacgcg cacggtgagc
     3241 gacccatcct ccaggcacat gggcaacgca tcgaagataa cctgctcggg gtagccctgt
     3301 tccgtgcgct ccacgagcaa gctgcgcgtg acccgcgccc cttgtctgta gaccgtgacc
     3361 gcatcgattc gagaaacccg ttccgcagaa cccatcacgt ctgacatcga tcccaactca
     3421 ggctagagcg gccaccgcgc tgagaccagt agcagatccg gcatccggag tcgctctgta
     3481 cagccgatga ctggagcacc gaacggtgct ctagctcggg ggcgccgttg tccacggatc
     3541 cggccctggg tttcggcccg ctacggccgc catcagggcc attccgtgat gggagcccga
     3601 tgaagggctg gctgctggtc ggacccgagg caaccggaac ggcgccgggc tcggcgcggc
     3661 aagactcccc gctgcttttc gggccgcccg agggaacggg cgcgacgcgc ggccgagtcg
     3721 cggcgtcagc gccccccttc gcggaatggc cccgccgctc taccgcgcaa gcggcgcacc
     3781 ttaacgacca cggcgtgtgc tcctccgatc agcgcggctg cgcgctcaac tggcgcaatg
     3841 atcacctgtc cacgacgttt cggtgcccgc ctacgagctc aactatgttt gagcaaagcc
     3901 tcgagttcgg cgacgcgggc caacgccgct tcggcgcgct ctgcgtcagt cgggaatagg
     3961 atatcgccat ttgggtcgag cgctaggcgc acgcgttgac gtggccgttc tgtgccgacc
     4021 aagcgcacga agcatccgag tactcgcgaa cgcacccggt ccgcctgggt gcgttcctgg
     4081 agctcgagcc ctcgcgaggt gcgttcgaag cgctgcagac gatagccctc tctgcgtttt
     4141 aggtaatcag gatctacgac gatgagctcc tcggttccga tttgatggtg cagccgtgga
     4201 gccgtctcgt agtccttgcg ccaatcctgg gagcagatct ccagagcgaa gctcggatga
     4261 atgccactct cccagatctt ccaggaaggg atatccgaat ccggaggaac gtcgggtagt
     4321 acgtaaatgt caggcgcaat gcgctgtgag acttggccct tctcgaagta gatgaactga
     4381 tcggagccga cgaaacacgg ggtgccgcgc tcggcgagcc agcgttcgat gagaggacgc
     4441 agcacttcga gcatccaaca ttgcaggatt tcttccccca cgggctcctc cacgggatac
     4501 accgtcgggt catcaggagt tgggggcgga gtgcgcatga gctcgaacga taccacgcgc
     4561 gtggcgcccc gccgaccggc agtgtgcatg cagggccatt tagcatgagg cgacgagaat
     4621 gcctgaacaa aagagcacaa aaacgcgagg gaatgcggga gtgtcccgca ggtcgaaagg
     4681 gcggcgcgag ggatatcccg agtgcagtta actcgaggta tcggcctccg ttgtgttcag
     4741 ccagggccaa ctcgattcga ttggattccg ttgctcacac tcggtcatgc ggcttcgtct
     4801 tcgagcgttg ctgtcgagag tgctagagcg gaaacggata gctggcttcg gcgacggcca
     4861 gctgcgcccc gctgagctcg aggtgtgacg tggagccgtc acgatacgtg acggtgactt
     4921 ggaagcgtga agggctgcgc aatagctccg tcgggacaag atagtaattc tcgatttgtg
     4981 ggcccgcgta ctcgaagcct ccgcggcgcg tcggctccgt caagttcagc cactgcacgg
     5041 aagcgacgct catgggcgga ttgcgaacct gcaccgcagc ccagtaagcg ttgctcccct
     5101 ccttcacgaa taacgcggtt ggaccgtcga atacctggct gtgtccaacg caggaaatcc
     5161 actcccaccc accgtcgtag cggccaaagc cgtccaacat caccgccgcg ggcgcggtgc
     5221 cgccgagatc gagaccgcaa aagctgtcgg gacacttgtc catgatgcgc acgacgacct
     5281 gcttcgggcc ctggctcgtg atcaccgtga ctcgcgcgca ctgcccacag atgcgccccg
     5341 cgtcccactg gcctcgagcg tccccaggtt cgaggttgac gtggatggca gcaaaggact
     5401 cgatcgcagt ggctccgtac agacacgcac ccccgttact cggcgaggtc gagtacatcg
     5461 tgacattgcc gaaggccgca cccccattgt ccacgacctc ggggggccgc ggcgtggctg
     5521 cgtcgcagtt cccaacgcaa cgggagggca cccctgcacg cgcgcccgct gccgtgttca
     5581 ccggctcaga aacgtcgtta ctgcccgccg gcacgcaggc caccaccccg agcatccccc
     5641 cgagccagca ccagcgagac cagtacatcg tctctcggtc ctagctcagc gcgcgttcct
     5701 agccagcccg cggccggttg cgatggctcc actcaccctg aagaggggag ggctgtgcac
     5761 ccgcggtggg caacgtgccg attcagaaat gacgagcgtc agcagccgtc ttagacgtag
     5821 ctgccgacgc cgggagcgct cacgttcaga tcgcgcagca cgacgctgaa ctcgggctga
     5881 tctctaccgc gttgaagccg ctcgaggcgc agcgccgttg agcagctggg gggctctcgg
     5941 gtctcttatc aatggagtgc ggtcaaacgg gatgtggtta aacagagtgc ggttaacaga
     6001 gtgcggcgag cgcggtattc gaacggtcgt ggggagaagt tgactgcgct ctcgcggcgg
     6061 tgcgacgcgg gggcgcgctc tttacaagga ggccgccatt ccagctcggg ggagatcggc
     6121 gagggaagtg aagacgcgcc cacatctagt ccgcgacctc gaaatccaaa gctccgtcgt
     6181 catgattccg tttgatcggt gctcgggttg gctagagttg ggagccatgg cgagtgaagg
     6241 actatagggg ggaacgctac gatcgcgcta agcgagaatc actgtctcgg agcccgtcat
     6301 gcacggcgtc cgagctcagg gtaaccaaaa aaatgtgtca cgacacaaca tcgccggatc
     6361 aagacgaggg gcaaacgcat ggccaccgtc gtgtctgccg ctattgtttg cggcgtccga
     6421 gtggacgggc gcgactccgc gttcgcgttc gtccagagcg cagccccatc tccccgagct
     6481 acccgcccac accgcgcaaa ccgcactcag caagcggcac ccgaacccgg ctcagtgcgg
     6541 cacgatgttc gagcgtgcgt gaatcccgag gcgcgtcccc gatgtcggac ttctctgcag
     6601 ctccgctcgc gcgcccgcga gcgcggcttc gcaggccacc ctcgagaaga accaacgttc
     6661 cttttcgttc gggtcgaacg ccagaacgtc atcggggaga actcggttgc tgacccaaag
     6721 ttcgtctttc aaggttttga gaacggtgta gttgctctca caggacgagc aattgagata
     6781 tcggttccca ccccaccgca ggctgccgaa ccagagctga gctgcctgat gaggggtcgg
     6841 gggaacgtac tcgatgggat agctgaggcc cttgccgtcc atggcctcct gcgattcgag
     6901 tttccccgtg agcacggggg caacgaggtc tttggggagt ggcacatcgt cgcggttctc
     6961 gcgcggccca gtgaacttct tgaagcgcca gcccgagcag aacaacttcc cctctgtcgc
     7021 gacgatccaa tagagttcac cgcgaccggc ccgcacccgc gatgataacg gctcaacgtt
     7081 ggtttgaggc cgagtgagca cctcgtagcc tgccggtatt tgcgcgacgc cctgcggttc
     7141 gaatccggga cggtgcaata gcagggtgcc cggtccctgt gtcaccactc ggcccgtaca
     7201 ccacgagacc gagcgagcaa cgatcgacga gtcgctgtcc aagttgtgat cgatgtcttc
     7261 gccgaaagtc tcgacgcctt cgatatactg ccgaaggagg tgctgcttac gcagcacgaa
     7321 cgtgtcatag cagggtgaga agctcacgtt gtaaagtgtt tcgctttggt tcgggatagt
     7381 tccccacaca ttgaatcccg agcgcaaggc ttcagagccg ggttcgggtt ggtattcgct
     7441 caggggcacc tccttggtcg acagcacctt gcgatcacag aaggctgccg tctgcccggc
     7501 tccgaatgaa ggcccgatcg atcccacacg aactcggctt gaattgctca gaaacgagcc
     7561 ctcccctgcg actgcaaccc gcaccaaagc acccggatga agtctcccaa agactggtcc
     7621 gtccggtgct ccgtagtgca ggtcagcccg ctcctgaacg taaagcgcca agccgcggcc
     7681 ctccgctttg gactgactgt ctcgtggtag ctcagcttca gctgccaaat ccttcgaatc
     7741 cggagtgcgg atacgtcccg ttggaagcaa ctccaccaat accgagtgtt gaagcggagc
     7801 actcagcact cccctcgttg cgccgggaag cgccggccct ggagtaagcc agaaacgcgg
     7861 agcccttggc gtcacgtctt tgcgggaact cgcactcgaa tctcgagtgt ggctccgctc
     7921 gcaaccccac gacgccgtca tgattgcgca cgtcaggaat aacgcccgcg cgcgccccac
     7981 ccgcacgcag ctcatcgcgg ccgctagctc acgattcatg ttcacttcac caaactcccg
     8041 aagccatcca cctctacgac cagcggcgac cttatcttcc cgaccttatc ttcccgacca
     8101 tgacctgacg agaggaagcc ttcctgctct gccccatgcg cagaacgaat gctgaacggg
     8161 acgagtcgga cggccgcacc gcgctccgtg acgacacctt tggagggaag ggggcaccgt
     8221 tcaccgcggc gaaaagcagt tggcgtttct ggaggcgtac gtcggggtgg tttttgcgcc
     8281 ccacgaccta tctacaatcc cacgcatgaa cgcgcgcgtc ctatggacca ttctgaccgt
     8341 ggtgggctgc gctcgaaccc agccaccacg accggtgccg gttcatccga gtcagccgct
     8401 caccgagtcg gccactgctg tgcggccgga tgtaatcgct aacgatggtg gcctcacggg
     8461 tccgatcgag tctctaggcg aggtgcagaa tgtcaatctg ctgcggtggg acgtgccgac
     8521 tccgaatgag cggcttgcct cggcggattc tcaacgacct tcgagcaaag cctccagctc
     8581 ggcgatgcgg gccaacgccg cttgggcgcg ttgcttctcg gcgtcggcgc gctggcgttg
     8641 ttcctccgcg cgttctgtgt cagtggggaa caggatatca ccatttgggt cgagcgctag
     8701 acgcacgcgt tggcgcggcc gttcggtgcc gaccaagcgc acgaagcatc cgagtactcg
     8761 cgaacgcacc cggtccgcct gcgtacgttc ttggagctcg agccctcgcg aggtgcgttc
     8821 gaagcgctgc agacgatagc cctctctgcg ttttaggtaa tctggatcta cgacgatgag
     8881 ctcctcggtt ccgatttgat ggtgcagccg gggagccacc tcgtagtcct tgcgccaatc
     8941 ctgggagcag atctccagag cgaagctcgg atgaatgccc ctttcccaga tcttccagga
     9001 cgggatatcc gaatccggag gaacgtcggg taagacgtaa atgtcaggcg caatgcgctg
     9061 tgagacttgg cccttctcga agtagatgaa ctgatcggag ccgacgaaac acggagtgcc
     9121 gcgctcggcg agccagcgct cgatgagagg acgcagcact tcgagcatcc agcattgcag
     9181 gatttcttcc cccacgggct cctccacggg atacaccgtc gggtcatccg gagttggggg
     9241 cggagtgcgc atgagctcga acgataccac gcgcgtggcg ccccgccgac cggcagtgtg
     9301 catgcagagc catctagcac gaggctagga gaatgcctga acaaaagagc acaaatttgc
     9361 gcaggaatca gggatatctc gcagctcgaa gagcaggcac tagggatatc ccgagtgccg
     9421 ctaattgtcg gctgggtaca ctgatgacca aacctggtgc ccaactccga taggtgagtg
     9481 tcagttcttg tgcctcctgt gccgtgatca tcggcgattt cgcgtggcgc tacccttcat
     9541 tgcgagtcgc ggcgagggag tcggcggcca taacgtccgc tcgagttgct cggccgtgca
     9601 tcgagaaaaa cggcaagaag gagaaatgac catggcccct cgacccgcat tcgtggctct
     9661 cctagtacta tggttctccg gcgctgcgtg gcgctgttca gtgcggttct cctcgggtac
     9721 ggcgggtgtg gctcgcgcat agcgagggcg gcagagcctc agtgcggcag tgggctcgtg
     9781 tatgtgggcc cgacgcgggc ggtgccgtcc aatgattcgc tcggggagtt gatcggcttt
     9841 cgcgtggcag atgggctgcg ctacgcggca acgggcgcga agacgcgcgt ggtgtcggct
     9901 tacgatttgg agttgcacgc aggcgctacc gcgcgtgcga tgcgaagtat ccatgcttgg
     9961 ttgcgtctgc ccgtggagca ggcgttcgag cggattttgg gggagcggcc gccgcccctt
    10021 tccgcctatg tgcagcccac cgtgattcgc gcggagaatt cactggtcat cgatctggac
    10081 ttgtattgcg tggaaacgaa ggcagagaag tcgcccgcca tcattagtaa gcgcaagtca
    10141 gtgcgtggct cagtagacaa ccagtccgcg ttgctggagc aggctttcac tgcggcctca
    10201 gcgttgtttc aagagggcga gccgcgtcga ccagtggcgc gggtggcggg cggcatccag
    10261 cagcggttcc cagtgggcga tccggtcgtg ctcgacgcag gtttgagtag tgacccggat
    10321 agtgacgctt tagtgttcga gtggacgggg cccgcagaga ttggatggcg gcaagaagga
    10381 gcccgcgtga cgttgtcccc atcgaggccg gggaagtacg cctttatggt gaaggtgtcc
    10441 aagctgctcg atcgttcgtc gagtgaatcg ttggttgtag ccgtgcaagt ggtcgcgccg
    10501 cccgtgattc gggtacaggg cggatcgatg gtagccatga atggaagaac gaaggtcgtt
    10561 ctcgatggca cctgcgaagg ctgcgaagat tggcgctgga ctcaggcatc cgggcccgtg
    10621 accacgctgc caagcacctg cggtccggac cgcagcgctg agatgcgtgg gccgcgtgtg
    10681 cctaccgaac ccggctcgga caacatgaca tcagctcgtt gcgaggtttg ggcggacgcg
    10741 ccggggcagt acgtcttcac cttagaggga gtgaacgcaa tcggttcggc gagcaaggac
    10801 gttactttcg gggtggcgcc cgcaccggtg gcgttgttcg atgcaccaga cgttgccgtg
    10861 gtgggccact cgtattcgct ggccgggact gccagtcacg acgtattcgg gggtaacctg
    10921 cggtatcact gggaggtcgc ggaccaagag tttccaaaac aaacctgtgc tcccatcaat
    10981 cggactctgc cggccggtgt ctctctctcc actccgaccc aggccgtaac cgagtttagt
    11041 gcggcacggc caggcaagta ttacgtgcgc ctcacggtat tggcgaatcg cacgttcggt
    11101 ggtcacgctt cggccaatca ggagtgccgg caacaagcaa tcttggtgga aaagccgatc
    11161 tggtggttca ccatcgaggc gggcggcgac ttcttaccgg agggggacat caaccgctat
    11221 tgggagggct ttcgcctgaa aggggcggtg acggtgcgcc cggcattcgc gcggaaggtg
    11281 ccgtggattg ggccttcact ggggcttcgc gtcgcgcaaa gcttcattca gtcgaatgat
    11341 caaggtaagg attttcggtt cggcggggga acttcgctcg gcttggctct gctgttctct
    11401 gactcaatcc aatttcatgc ctcagcgttc gcgcgaacct ggggaaagac ctccgtaggg
    11461 cccgagatgg ggctcgaatt cagggttcaa cgcatgctcg tgctcagcgc cgatgtacgg
    11521 gcggaacgtc gggacaatga atggcatgtg ccggtagtcg gcggcttctt cggtgcaggt
    11581 tacgacctat gaaacaccca gctcgcgccc tctgtttcct cgccgccgcg gccctctttg
    11641 cccccggcgc gcacgccgac cccttgctcg tggaagggcc gtcgttacca acggcagagc
    11701 ttgaatggat ccgggggcag ctggtgaatg gctcgttcgg cggcgcgtcc cagtcactgc
    11761 cgcccggcgt aagcgtgacg gttaaactca ccgtggagca gggccagtgg tgtgctcgcg
    11821 gctcgctgag cgcctgggac gagcccacac cggaggaact cgagaagcgc tgctcagcaa
    11881 tgggtacctc cctgctcggc actcagagat atgcaccttc ccatgcggcc ctctcgcgat
    11941 tcatcatcga gccactcgcg agcgatatca cccggcgctt gtcctcacgc tggcagctgc
    12001 gcatagcgca tacaacgcca gagtccctca acatcagcgt gggtgaccgt gacgcaaaac
    12061 cctatctcgc catgagcgag actggcgtcg ccaccctctc ggtcaagtct ggtagcaagg
    12121 tcaccgtcag tgctgggtcg tgcactcgca ttctggacgc cgcgacgccg atcatcgatc
    12181 tggagcaact ccgtaccgtg cgaaccttct caccggtgtg gacccgcgaa ggacaagcca
    12241 ttcagtcgct cacgctggac accggtgatg tcacccggct cacgttgcgg ctcgatgagg
    12301 agagcaacca gggagccatt gccctacctt gctccggagc aatctccgcg cgaatcatgg
    12361 gcgagacatt cagcgccacc tcaatcgaga ccaagcccga aagttggact tggtcaatcg
    12421 aagccaagaa atctcccggg atcatccacg ttggcctccg cgctgccgag ggccaaagcg
    12481 acaaagaaac cgcgattagc ctaagcttcg agccgccctg gtggacgcgc ctatgggtca
    12541 aggtctcggg ggcactactc ggagcgggcg cgctgctcaa aggcattctc gaaatcaaga
    12601 aatcgattca ggacctacga ggcaagaaga actccccaac tgagaaagcg ccgcaggagt
    12661 aggagagatg aacccttcga agtcgcggga cacgcttcaa tcctctagct ttgagtgcgc
    12721 tttccgtaca gtcctgtacg gccatgaccc gctgttctgg gctgagctcg tcccagaact
    12781 ctgcgtccag cgctgctgcc cgctcaactg gcgcaacgac cacggtccac gctcggtcga
    12841 gcctgtcccg gcgtttttga cgtttcgtgg tggctacgct gcctcaggga gctcgataga
    12901 acgaggcgcg ctgcaacgaa acaccggaag cccccgcgct ccttgcaaag acgagatgca
    12961 ggcttcatcg tgaaaccaca tgcagccgcc gccctctgtc cgcgaccctc acgaacctga
    13021 ggttcctcca gccgaacctg cgctcgcgag tgcttcgagc gcgcccgcgg gcagcggttc
    13081 ggtgttgcgc ggcggcctcg tcgccttggc gatcggggtc gtcgcgaagg gtaaggggct
    13141 gtggatcggg ctcaaggcac tgccgctcgc gaaggtgttc ctcacgggcg gtagcatgat
    13201 cgcgtcggtg ctcgcctatg ccatccatgg cgggtttgcg tttggcgtgg ggctggttct
    13261 catgatcttg atccatgagc tcggccatgg cttcgcgatg aagcgcgctg gcgtagactc
    13321 aggttggccc gtgttcatcc cgttcttcgg ggcgatgatc gcgatgaagg gcaagcccga
    13381 gcacccacgc gcagaagcgg cgatcgccta tggtggcccg ctcgccggta cggccgcagc
    13441 gctctcgtgc gctgcgctcg gcttggtctt gcggagcccc ttctttctgg cgctcgccta
    13501 caccggcttc tttctcaatc tattcaacct cgcgccgttt ggatttctcg acggcggccg
    13561 catcgcgcgt gtgatctcgc gcaacgcctg gattgccgga gcgatcatct tgggcggcat
    13621 gtgtttgatg agtccgtcgc cgcagctcat cctcatcgcc gtgctgggcg ccgcgcaagc
    13681 gttccgccgc gacaataccg atctcgaacg agtcaccgac gatgaccggc gcgcttgggc
    13741 gtttcgctac ttcggcctat gcggcttctt ggggttggcg gtcgtattct cgcatcagct
    13801 cacgcgtgct cacggctggt gatgaggcga gctcaggacg cgccccccac tgaacagagc
    13861 caagccgttg tgccgaggcg cgtaaggcgt cgcaagagct ccacggcgaa ggtgcggatg
    13921 ctcgagctgc cgttgtgcta atcacagggt gatggcggcg tgtggaaaac gaaggttcgc
    13981 cctcttcgga ggcctgttgc tcgcgtgcgg cgcaccctcg ggcggcgcac cctcgggagg
    14041 tcgggagctc actcgcgagg aaattccggt gctggagccg ggctgggaca tccccatcgt
    14101 ggatgagagt gcgctgcccg aacccgcgcc tgaggccggc ttcatcgccc tggagaagac
    14161 ggcttatcag gcacgcctcg aaggcgatcg tgagttcacg agtgaacccg cccgtctgtt
    14221 ctacaacttc gtgccggcgg agacgggagc gaaggagaag ccggtcatcg tgttgttcaa
    14281 cggggggccg ggtgcgcccg cgacgctgct tcgatgcttt ggtacggggc cgaacagcct
    14341 cgacgcggag gatctcagtg ctccacccgc tcccaatccg taccgcttca cccgctttgg
    14401 gagtttgctc tacatcgatc cgcgcttttc cgggttctcg tacggcgtca ccgagcaccc
    14461 cgaatcggac gccgaacgtg agcaggcctt tcgggaagac agcctgaacc cgacgcggga
    14521 cgcggccgat tttgcccaag tcatcctgcg cgttctcaac aagatccccg ccatccgcaa
    14581 caaccctgtg gtgttagttg gcgagagcta cggagggacg cgggcggcgc tgatgctgga
    14641 cattctcacc gaccttgggg acgatttctc aatctatgtg aaccaggcct ggaaggctga
    14701 gctcgatgag cacttcgcgt cggtgttcgc gcgcccggag cgcgcgaatc gggccagccg
    14761 ggccaagcaa ttcggttggc agatcctgat tcagccgctg ctgttcggcg ccgcccaata
    14821 cgacaagatg ggggatttct ctcagcgcct gtacagagat ccagccgggc cgtgccgtta
    14881 tcacgtaggc cgaaccggtg cctggtgtga agagctcgag cgggcggcga cacggactat
    14941 ggtcgacccc aagtctttcg agaccattgt gggcgtgagt ccgcaacgag tccccggctt
    15001 ggccgcgagc gaccgagacg gcgccttccg tggagctcgc tccgaagcga cgtcggagac
    15061 gggcttcgag gagctactcg gcgggttacc gacctgggac gcctactata cagaccaaat
    15121 ctccgtggcg ctcggaaacc tgacgcgaac gaccgagatt tactcctacg tccataccgc
    15181 cctccgttca ttgacgcaaa cgcagaccct gatcacgaat gcgctgatgg atggcgtcat
    15241 cgagtcttcc agcattctcg aggtgctcgc gtcgatggac ggcattcaga tcgagagtgt
    15301 cgcgatgccc tccaccgcac aggcggacac gatatcaccc gcggatcgcc ccgtcgatcg
    15361 tctttatacc gtcaggtatc ctgccgcgga tcagacgcgt gaattcaccc tcttcttccc
    15421 cgtttacccc gacagcggcc acgcggtgac cgtcagccag cccgacaagt tcttcgccga
    15481 cgtgcagcac tttttagcgt ccaccggcct cggacggtag gaaccaccag gttggtgttc
    15541 gatcgtgccg cctgcgcggt gctctgaggg catcgagcga aagcggctgc gctcgggtcg
    15601 aaggcggagg ggattcgcgc ccgtcaccgc ggggcggaat cacaaaaagc gagatcgcga
    15661 cttctcttgg cgagcttggg tttgcggcgt ccgagtggac gggcgcgacc ccgcgtccgt
    15721 gtcgtccaga gcgcagccac atctcgccga tcaacccgcc acaccgcgca aaccgcactc
    15781 aacaaccacg acgcaacatc gccgaatcca gatcgatgtt gcaagaggtc cgcgcctcgg
    15841 acggtcgcag cttccctagt gcctgcccgg ccgctactcg aggcaccggt tcaggaggat
    15901 tctgacgtcg ttattgttgt cgttgggatt ggtgacggcg agatcgagcc gcccgtcacg
    15961 gtcgaagtcc gcggccgcga gcgaggtggg gccgcggcca gccgcgtaat gcgaagtgtt
    16021 gaagcgccct gctccttggt tgagaaggac agtgatattg ccttggctgt tcgcaactgc
    16081 caagtctgct cgagagtcgt tgttcagatc gagtcgagcg agcccggtcg gtacacgacc
    16141 aacgggatag ctgaccggcg ctcccaatat gccgtcgccc gtgttcaaca ggatgtagat
    16201 ttgacctacg atgtctgtgc ccgtgtaact cgcgaccgcc acgtcgggct tcccgtcgcc
    16261 attccaatcc ccgtgggtga tcgctccggg agctgcgggg agtgcatagg tcacggggga
    16321 ggagagccca ccctctccca cgttgaatag tacgctgacg gtttcacgag gcgggttcgc
    16381 cggaaaacca cttccgggaa tgaaggcgcc ggagttgctc acgatgatgt ctgtgtggcc
    16441 gtctccgttc agatcggtgg cggtcaatgc cgtcggagcc acccccgagc ggaagttctt
    16501 ggctgcagcg aacctgccgt cccctcgatt cacgaacacg ctcacgtcat tgctgccgaa
    16561 gttgactgcc gcgaggtccg gcgcaccatc accattcaaa tccgctgcga tgacgctttc
    16621 ggtgcgttgg cccgcgacgt aggagtccga gtgattgagc cgaccagtac cgtcgttgag
    16681 caagacttcg cagccatccc ccgccaagac tacgtcgtcc cagccgtcgc cgttcagatc
    16741 ggcggcgatc aagcgcttgg cggtcgtttg cgtggtgtgg ttttcggccg gagcaaatgc
    16801 tcctccgccg tcacccgtga acacgctcat tttttgagtg ttcgcgttga tgacggccaa
    16861 atcgctgcgc ccatcgtggt tgaagtcagc ggcggcgacc gaatccgggt acctcacggg
    16921 gtcgaaactt cgcgtggggt tcaagctgtg tggcgccgcg aagctgccgt gttggtcgtt
    16981 gatgagcaca ctcaagtcgt ccgtgctttc gttgacgacc gccagatccg cgctcccatc
    17041 cccatctaga tctgcgactg ccagcgccca agtttgagct ccgccggcat actcacgccg
    17101 tgaggcgaag gtcccgtcac ccagattacg aagtaccgct gttacattca cataagggct
    17161 caccaccgcg agttcgggtt ttcgatcgcc gtccaaatcc gcggcgacga gggagtgcgg
    17221 tccggacggg gcctcgtagt gcgttgcgga agcgaaagtc ccatccttac gaccgagcag
    17281 caccgcgatc tcgcttgcgg gctcactcgc cagagcaatg tcggatgcgc cgtctccgtt
    17341 caagtcggcc acactcaacg aggtcgagct gagggccggt ggatcttggt cggggatccc
    17401 ggtgaagtgg ctactcggtg cctcgaaggt accgtccccc ctgccaacga agagcttcaa
    17461 ctcagtgcca tacgaaggaa tcaacgcgag gtccactcgg ccatcgtcat tgaagtcagc
    17521 ggccccgata gcccccgccg accacggatc tgcggaactc gccatgagcc tgaagcgacc
    17581 tccgccttgg cccaatagga cgttcaatcc gccggacccc gttaccgcca gatcggcgcg
    17641 gccatctccg tttaaatcgc tgatcgtgac ggcgcgcggc aacaccgtga gttcggccgt
    17701 atacgtcgag tccaattcga acttcccgtc cccgagattg atgaggaaga ccaggctttg
    17761 ggacggatcg ctcgtgacga tgaccagatc gagcgcgcca tccccgtcga catctcccgc
    17821 caccgcggaa gtgggctgca gcggaacgct gtactcggtg attggcgcga actttccggc
    17881 gccctgactc aagagcacgc ccacactgtt cgcgacggca ccgaccacag ccaaatcagc
    17941 gcgccgatcc ccgtcccagt caccggaaac aacgtagcgc ggactgcgtg cgaccggcac
    18001 cgagggcaaa cccggcaatc cgagcgtgcc ctcgcacttc ggcgcttcgc cgctcggcat
    18061 ccccgcagct cccgcggagc ccgagctccc agcgtccgac cctccaccgc tctcgcccgc
    18121 tccagcgtcc tcaccagcgc ttccgccgag gtgcgagcct ccttgggcgt ctccgcctcc
    18181 gtgggcgctt ccgccgaggt gcgagcctcc tccttgggcg cccccgtcag cgctgccgcc
    18241 cgttactggc tcggactcgc cgctcgaccc acctgtgctt gttatccccc cgctgcttgg
    18301 cccgtctgtg cctgcgccgc cttctcctcc cgtcataccg ccttccgcgt gagtgccacc
    18361 tgattgcgtg ccacccctat cgtcagagcc cgagtgagag cctccgcgct cgttttgacc
    18421 ttgagtcgcg ccgccggagt gaggttgagc tccaccttga tgcccagccg ctcccgaggc
    18481 gcccgcaccc acatcaggct gagaggaggc cgcgttgcag ccgatcaagc acccgagcag
    18541 cagaaaacgt gaaggtgagt gaagggatcg attcatggtt ttcccgagac aatcgtccag
    18601 aatgagcgcc tgccgcccga tggacattcc tactcgcatc tccaccgcag aatgaagctg
    18661 ccggaagcga ttgttacgat tctttggtga acgcgagttc tgcccgggat aaaccaaggc
    18721 ttccgcgcgg caaaccgtcg gtgtccgcgt cgttgacgcg cagacccgac ggccgcatgg
    18781 tgcgatgatg aattccgtgc cgggcgctcg caacttcgtg gctacgggtg agacctacga
    18841 cttgttcatg gggcggtact ctcgcccgtt ggcagtcctg ttcgctgacg ccgccgatct
    18901 cgcccctggg atgaaggctc tcgacgttgg atgtggcccc ggcgccctta cgggtgttct
    18961 ggttgaccgt ctcggtgccg ctaacgtcgc cgcaatcgac ccgtcaccca cgttcgtgga
    19021 agcatgcaaa gcacgccacc gggaagttga cgtgcgatcc gggcgtgccg aaaccatccc
    19081 gttcgaagac cagagcttcg accgcgtgct agcgcagctg gtgctgcact tcgtgagcga
    19141 gccagcgcgg gctgcgcagg aactccgccg ggtactgcgt cccggaggca tggctttggc
    19201 gtgcgtttgg gacttcacgg caggcatgga gatgctgcgg cacttctggg atgccgcgct
    19261 cgcgctcaat ccagaagctc cggacgaagc gcgcattctc cgcttcggtc gcgagcgtga
    19321 gattctcgat ctgtttgcgg ccaccgggtt cgagaacctg accgaaacca ccctgcaagt
    19381 cacgtcttcc tacatagact ttgacgagct ctggtctggc ttcctcgcgg gcatcggtcc
    19441 ggccggcgca tactgcatga gcctctccga ggaggagcgc gccacactac gcgctgagct
    19501 attctctcgc ctcggacagc cacgcggagc cttctcactc ggagcaatgg cccgatgtgc
    19561 ctccggtcgc gcaccagcgt gactgacaaa cttcgtgggt atggttcggc gtcagagtcg
    19621 acgggcgcgc ccccgcgtcc ctgtccggca aaagcgcagt cccatctccc cgagttgccc
    19681 atccctaccg cgcaaaccgc actcagcaac ggacggcccc gcgagcacgg agagtcgcac
    19741 cttgctagag gatcggtgtc aaacggtcga gttcggctca atatcgagtg atgggggcaa
    19801 cggcgcga
//
LOCUS       DASTKU010000056         4863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_232467, whole genome shotgun sequence.
ACCESSION   DASTKU010000056 DASTKU010000000
VERSION     DASTKU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232467"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFQ61_01890"
     CDS             complement(<1..320)
                     /locus_tag="VFQ61_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9953221.1"
                     /translation="MIQTGEVLAGKYRVEQVLGKGGMGYIVSAIHTQLNERVAVKFMM
                     PELCEHQEAVARFLREARAAVRIRSEHVARVRDVCTLHDGTPYTVMEFLSGHDLATEL
                     ERVH"
     gene            complement(568..1785)
                     /locus_tag="VFQ61_01895"
     CDS             complement(568..1785)
                     /locus_tag="VFQ61_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953222.1"
                     /translation="MRSTKALVSVLISSLALVLAAGCGDDDDDVVASGGKGGSSGGSG
                     GSRAGSGGTGGRSGGATGTGSSSGGMGTSGGASSGGTLATGGATPTTGGVTSAGGSTG
                     GQVNLGGTTAVLEGGSAGEAGVGGESGGSAGSGGSDPVGGGGAGGEAGAESGGTTSSG
                     GTAPTGGTGGTSSGGTSSGGTSGGPDYCRGKCATDADCKNSKGSWGYCEPSTHYCRGT
                     VLCDSATDCIPSASGWTVRCSAELACPNAGDVCVTAPTDKFQLGFCASPKPMDADCAA
                     VDRSALTLKRFGTNQDVTVCANTTTQGCNAGVCTAKTCAADGECNASKGGSSCDEAKG
                     FCECAADTDCGETGRTHCYTDTKRCGCSTDAECAQAGRTRICNLNTRACETVVACQDA
                     SACTSTYPNMVCE"
     gene            2233..3732
                     /locus_tag="VFQ61_01900"
     CDS             2233..3732
                     /locus_tag="VFQ61_01900"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9953223.1"
                     /translation="MTEERRAKDPLPSTLSLRLDSSEESNRRYMLVLVSDSGLTRIHL
                     DPNVAVRIGRRKDCDIVLDDPSASREHAVIHPGEPPSIEDLGSANGTYLQGERLTPRK
                     PVLLAPGMVIDVASLTLIVRDSQTAATHIRETRAASASAPPTHGVVLRDPKMQELMRV
                     ASKAAKSPMPLLILGETGVGKEVLTERIHELSSRGERPLLRVNCATLADGVLNSELFG
                     HERGAFTGAVGSKPGLFEAAHTGTLFLDEVGEMALETQARLLRVLETGEVMRVGGTQP
                     RKVDVRVISATNRDLRRLAGEYRFRADLYYRLNGLTLHVPPLRERPLDILPLANFFAV
                     RTATLMALPPPTFANDAEGALLRYPWPGNVRELKHVVERAVVLSPSSILDASALQFDG
                     GVPSHPPSSDPSQAPHSTRGPESSRFHEGERASRSTRPATHSSRLRSSRKADALRAEL
                     QRAERDRVLEALQQGGTQADAARLLGISRRALLYRLDALGIPRPRKGRV"
     gene            complement(3749..>4863)
                     /locus_tag="VFQ61_01905"
     CDS             complement(3749..>4863)
                     /locus_tag="VFQ61_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET9953224.1"
                     /translation="CSGVAAAWSGQASRIVAGAFSGAALLFAVLGPKWNLAELTSGAN
                     VYFEGPKTTQELVSIREDVQGGVTTVTLSEGVHTLFTNGKFQGNTGWEMQAQRLFAHY
                     PCLFVQNFDQALVIGLGTGTTLGTLAAYPFKKLYVAEISPGIVEAAGKYFRAPNRDAL
                     RDPRVELSLSDGRNYLLLSERKYDLIGMELSSIWFAGASNLYSREFYELVRSHLEPDG
                     IFQQWVQLHHVYQRDFATVVNTLRRVFPHVTLFYGGGQGILVASLRPQRASESRLAQL
                     ESTRDLRAVEPLGRSLPELLDDVLVRDQEIDAFLASISTEIGVPLEKLVSTDENLYLE
                     YATPRGNVLPWSTREALVERLRSHHSAASTSALLVP"
BASE COUNT          886 a         1614 c         1472 g          891 t
ORIGIN      
        1 tggtgaacgc gctcgagctc tgtcgctaga tcatgtccgg acaggaactc catcacggtg
       61 tatggcgtgc cgtcgtgcag agtacacacg tcgcgaacgc gcgcgacgtg ctcgcttcgg
      121 atccgaacag cggcacgagc ctcgcgcaag aagcgagcga cggcttcttg atgctcacag
      181 agctcgggca tcatgaactt gaccgccacg cgctcgttca gctgcgtgtg aatggccgag
      241 acgatgtagc ccatgccgcc tttgccgagc acttgctcga cgcggtattt gccagcgagg
      301 acttcgcccg tttgaatcac agaacccgaa ctatatcatg gtcatttggt taggcacggc
      361 agctttgacc gaggtttcgt ccgcatctag tggatgctac cggcctgaga gaagtttgcg
      421 ggctcgggat tggaaccaac aagccggata aaaggtgccg gctcgctcac ccgacgggtg
      481 gagcggcgcg ttggaggtcg gcgcgccgcc ggacctccaa ccttccgagc tcgaccttcc
      541 gagctagttc gtgctggagt agttagctta ctcgcagacc atgttcgggt aggtcgaggt
      601 gcaggcgctc gcgtcctgac acgcgacgac ggtctcgcag gcgcgggtgt tgagattgca
      661 gatgcgcgtg cggcccgcct gcgcgcattc cgcgtccgtt gagcagccgc agcgcttggt
      721 gtccgtatag cagtgcgtac gtcccgtctc cccgcaatcg gtatcggcgg cacactcgca
      781 gaatcccttg gcttcgtcgc agctacttcc gcccttcgat gcgttgcatt cgccgtcggc
      841 agcgcacgtt ttagcggtgc acacaccggc gttgcagcct tgcgtcgtgg tgttcgcaca
      901 gaccgtcacg tcttggttcg tgccgaagcg cttgagggtc aaggcgctgc gatctaccgc
      961 ggcgcagtcc gcgtccatcg gcttgggaga ggcacagaac ccgagctgaa acttatcggt
     1021 cggagccgta acgcagacat cgccggcgtt cgggcacgcc agctccgcgc tacatcgcac
     1081 cgtccaacca ctggcagacg ggatgcagtc ggtcgcgctg tcgcacagca ccgtgcctcg
     1141 acagtagtgg gtcgacggct cgcagtagcc ccagcttcct ttgctgttct tacagtcggc
     1201 atcggtcgcg catttgccac ggcagtagtc cggaccgccc gaagtgccgc cgctcgaggt
     1261 gccgccgctc gaggtgccgc cagtgccacc cgtgggcgca gtgccgccac tcgaggtagt
     1321 tccgccgctt tccgctcccg cctcgccgcc agcccctccg ccgcccacgg gatccgaacc
     1381 accgctgccg gcagatccgc cgctctcgcc tccaacaccc gcctctccgg cgctgccacc
     1441 ttcgagcacg gccgtggttc cgccgagatt cacttggcca cccgtggacc cacccgcgct
     1501 cgtgactccg ccggtggtgg gagttgcgcc gccggtcgcg agagtaccgc cgctgctggc
     1561 gccgccacta gtgcccatgc ctccactact ggaaccagtt ccagtagctc cgccgctccg
     1621 accgccggtg ccgccggatc cagcgcggct gccgccggaa ccgccagagc ttccgccttt
     1681 gccgccgctg gcaaccacat catcgtcgtc gtcaccgcag ccggcggcga gaacgagagc
     1741 caatgagcta atgaggactg atacgagtgc cttggttgaa cgcatggtga tttctccctc
     1801 gcctgtgcgc ggcccttgtc ccatgctcgt gagcgcgatg caataggcgg cgccgcaagt
     1861 tcatacgctg atcgggctcg ccacgccgct cccttcgcac tgttcaaagg actcaccaac
     1921 gtcgcgttcg acgtatttca cctacgcgga atagagtcgg cagctctcgc gggatacagt
     1981 cagcacaggt tcgcgcgccg cgcgcgaacc gtgcgttcgc gcgactgcag ggcgtaactg
     2041 cgcgcacgaa tggtcattta ttcgtgggaa ttgagccgct tcgcttgccc gaactcacgg
     2101 ctgtctgcac tctatgagcg ctgagaagac cgggcctagg ctccggcggc cgcactgcta
     2161 tgctgtgccc gctcgcagct atcgcgggct gttgccagca accccttccc tacaaattta
     2221 accccgcagc gcatgaccga agagcgccga gccaaagacc cactgccctc cacgcttagc
     2281 ctgcgtctgg attccagtga ggagagcaat cgccgctaca tgctcgtact ggtcagtgac
     2341 tcggggctga cacgcatcca cctcgatccg aacgtcgccg tgcgcattgg gcgccgaaaa
     2401 gattgcgaca tcgtgttgga cgatccgtcc gcgtcacgcg agcacgcggt gatccacccg
     2461 ggtgaaccgc cctcaatcga agacctcggc agcgcgaacg ggacttactt gcaaggggaa
     2521 cggctcacgc cacgcaagcc cgtgttgctc gcgccaggga tggtgattga tgtcgcgagt
     2581 ctgacattaa tcgtgcgaga tagtcagacc gcggccacac atattcgtga aactcgcgct
     2641 gccagcgcga gtgctccgcc cacgcacggc gtggtgctgc gcgatccgaa gatgcaggag
     2701 ctcatgcgcg tggcctcgaa ggcggccaaa agcccgatgc ccctgctcat cctcggcgaa
     2761 acgggcgtgg gtaaagaggt gcttaccgag cgtattcacg agctctcgag tcgtggggaa
     2821 cgtccccttc tacgagtcaa ctgcgccacg ctcgcggacg gagtcttgaa tagcgagttg
     2881 ttcgggcatg aacgcggtgc gttcacgggc gcggtggggt cgaagcccgg tctgttcgag
     2941 gctgcgcaca ccggcacctt gtttctggac gaagtcggag agatggcgct ggagacccaa
     3001 gcgcgcttgt tgagagtttt ggagacgggg gaagtgatgc gcgtgggcgg cacgcagccc
     3061 cgcaaggtgg acgtgagggt catttcggca actaaccgtg acttgcggcg attggcggga
     3121 gaataccgct tccgtgctga cctatactac cggctcaacg ggctcacctt gcacgtgcct
     3181 ccgctgcgag agcgccctct cgatatcttg ccgctggcca atttcttcgc cgtgcggacc
     3241 gcaaccttga tggccttgcc ccctccgact ttcgcgaacg acgcggaagg cgcgctgctg
     3301 cgttatccat ggcccggcaa cgtgcgcgag ctcaagcacg tcgtagaacg cgccgtcgtg
     3361 ctctccccgt ccagcatttt ggacgcaagt gcgctgcagt tcgatggcgg agtgccgagc
     3421 catcccccgt cttcggatcc ctctcaagcc ccgcattcga cacgcggtcc ggagtcctcc
     3481 cgctttcacg aaggcgagcg cgcgtcgcgc tccactcgac ctgccaccca cagctctcgc
     3541 ctacggagtt cgcggaaagc cgatgcgttg cgcgccgagc tgcaacgcgc ggagcgagat
     3601 cgggtactcg aagcactgca gcaaggaggc acccaggccg atgccgcacg cctgctcggg
     3661 atttccagac gcgctttgtt gtaccggctg gatgcgctcg gcatcccgag accgcgcaaa
     3721 ggccgggtct agtcgatcgc ggaagccatc acggcacgag caacgcagac gtgctcgctg
     3781 cggaatgatg ggaacggagc cgctcgacca gtgcctcccg ggttgaccat ggcaacacat
     3841 tcccgcgtgg cgttgcgtac tcgagatata gattctcatc ggtcgagacg agcttctcga
     3901 gcggaactcc gatctcggtc gaaatcgagg ccaagaatgc gtcaatctcc tgatcgcgca
     3961 ccagaacgtc atcgagcaac tctgggagcg atcgcccgag cggttccacc gcgcgcaaat
     4021 cgcgagtcga ctcgagctgc gctagccgcg actccgaagc ccgctgcggc cgtaaagacg
     4081 cgaccaagat gccctggccc cccccataga acagcgtcac gtgcgggaaa actcgacgta
     4141 gagtgttgac caccgtcgca aaatcacgct gatacacgtg atgaagctgg acccactgct
     4201 gaaaaatgcc gtccggctcg agatgcgagc ggaccagctc gtagaactcg cggctataca
     4261 ggttagaagc gcctgcgaac cagatgctgg atagctccat cccgatcagg tcgtacttgc
     4321 gctcactcaa cagcagataa ttacgaccgt ccgaaaggct caactcgacc cgcggatctc
     4381 gcaacgcgtc gcggttcggc gcccggaagt acttgcccgc agcctccacg atgccgggag
     4441 aaatctcggc cacatacagc ttcttgaagg gataagcggc gagggttccg agcgttgtcc
     4501 ccgtacccaa accaatcacc aaggcctggt cgaagttctg gacgaacagg cacgggtaat
     4561 gcgcgaagag ccgctgagcc tgcatctccc agcccgtgtt gccttggaac ttgccattgg
     4621 taaatagcgt gtgaacccct tccgagagcg tgacggtggt cactccgccc tgcacgtctt
     4681 ctcggatact cacgagctcc tgagtcgtct tcgggccttc gaagtatacg ttggcgccgc
     4741 tcgtgagctc ggccagattc cacttcggtc cgagcaccgc aaacagcagc gccgcacccg
     4801 agaacgcgcc cgccacgatc ctgctcgctt gtcctgacca agccgcggcc actccggaac
     4861 acg
//
LOCUS       DASTKU010000057         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1125414, whole genome shotgun sequence.
ACCESSION   DASTKU010000057 DASTKU010000000
VERSION     DASTKU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125414"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFQ61_01910"
     CDS             complement(<1..373)
                     /locus_tag="VFQ61_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953225.1"
                     /translation="MLNVSKYFFALAAVVSITAVGCDDGGGTQAATGGSGGGTQTAMG
                     GSGGGGTTSAPPTGEAIALTPNEMGWVDLTTNTVGIQGAWYPYGDNAGDKKCTATGGH
                     TDAECSVIAKPDPTVMGFPNTG"
     gene            complement(714..1070)
                     /locus_tag="VFQ61_01915"
     CDS             complement(714..1070)
                     /locus_tag="VFQ61_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET9953226.1"
                     /translation="MAVRVFQYAKCGTCRKALALLDALGVEYETVDIVTAPPSREQLA
                     KVLDESGLPIAKLFNTSGQSYRDGGYKERLPKLSHDQALRELAADGKLIKRPIVIGNG
                     FALVGFDEASYRRRFG"
     gene            1241..1693
                     /locus_tag="VFQ61_01920"
     CDS             1241..1693
                     /locus_tag="VFQ61_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953227.1"
                     /translation="MIRSVLAPRSIRRLALTTALVIVAACSRQGEGERCDSTVNGNAD
                     CEGDLQCKAHGSLATGDVDRCCPPGSLFNDVRCTPGSGVSTGTGGNTNGSGGDTSSEG
                     GATSSESGGSTSAASGGDTSSTASGGASTATTETSGGQMSQAGSSSES"
     gene            1697..2947
                     /gene="epmA"
                     /locus_tag="VFQ61_01925"
     CDS             1697..2947
                     /gene="epmA"
                     /locus_tag="VFQ61_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HET9953228.1"
                     /translation="MTEVVRAEPTVHPAQPLLFPSDVRDGRTRIADEEQRIAGRVLLV
                     QAETLVIADAFASLRVRCSTHLEVNEGDWVIVRGQLGSDELSAARLEFHQSAPAPHGS
                     GEVGRFVFSGVGTHLAARARALREVRDYFQDRGFTEVETPWRVPAPGVDLHVEAFRAD
                     AGYLITSPELEMKRLLVGGVPKQFQFARVSRADEAGRLHEAEFTLLEWYRAFTSYEAV
                     QEDTEAIVRRVATRIGANGALTTPTGRRVAVEQTFERITVSEAFRTYAGVTDVSELAQ
                     RDEARYFELLIEQVEPALSRCERPIFLVEYPISQAALAQPCAHDARFAERFELYAGGV
                     ELCNGYGELTCPKEQRRRFEAEQTRRRDGGLQVYPINERFLSALAEGMPPSAGNALGF
                     DRLLMLALGARRIQDVMAFPRERA"
     gene            complement(3011..3682)
                     /locus_tag="VFQ61_01930"
     CDS             complement(3011..3682)
                     /locus_tag="VFQ61_01930"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET9953229.1"
                     /translation="MATRKGTIVKRSKPVPARLLPTPRGVEPKKPAPAPERHKLDEIS
                     LPDEFDALSYVRALIREVPDFPEPSVLFRDITPLLADPKGFHIVLDAIAHRFVGSQVD
                     AVVGVESRGFIFGGALAARLNASFVPVRKPGKLPFRTDKVSYSMEYGEAELEMHRDSL
                     REGASVVIVDDLLATGSTAAACGELVHRQGAYVLAYAFVIELSSLGGRERLLPVPTVS
                     IVRFD"
BASE COUNT          684 a         1272 c         1233 g          699 t
ORIGIN      
        1 cgccggtgtt cgggaagccc atcacggtcg gatcgggctt ggcgatgacc gaacactccg
       61 cgtccgtgtg cccgcccgtg gcggtgcact tcttgtcccc ggcgttgtcg ccgtaggggt
      121 accacgcacc ctggatgccc acggtgttcg tggtcagatc gacccagccc atttcgttgg
      181 gcgtcaacgc gatcgcctcg cccgtgggcg gtgcgctcgt ggttccacct ccaccgctgc
      241 cacccatggc agtctgcgtg cccccgccag acccacccgt cgcagcttga gtaccgccgc
      301 cgtcgtcaca gcccaccgcg gtgatcgaga ccacggccgc aagcgcgaaa aaatacttgg
      361 agacattcaa catgtggagt tttcccttct aaccaaaaag gctgctcggt cctcgcaacc
      421 gcccccgaac tcgcaagagc tttccgccgg ctcgtacgtg gcgaagagcc gcgcgcgaga
      481 tggccgagtg gaggctgttg gcgaagcaca gagccaagaa gcccggggag tctggctcag
      541 gtcgcgcggc aaagcaactc ctcgacgacc caggctttgc gcggattttc gagcatttcc
      601 agccattgtc cgagccattt tcggagtgcg gcgcggcttt ctgcctagct ttccaggcga
      661 catgagcttc gtcgctggtg ttccccgttc agcacccaga ccgtcgacaa gggtcagccg
      721 aagcggcgac ggtagctggc ttcgtcaaaa cccacgaggg caaagccgtt gccgatcaca
      781 atggggcgtt tgatcagctt gccgtccgcc gccagttctc gcagcgcctg atcgtgagac
      841 agcttcggca agcgctcctt ataacccccg tcccggtagc tttgacccga tgtgttgaac
      901 agcttggcga tcgggagtcc actttcgtcg agcaccttcg cgagctgctc acggctgggt
      961 ggtgcagtga cgatgtctac ggtttcgtat tcgacgccta acgcatcgag caaagccaga
     1021 gctttgcggc acgttccgca cttcgcgtac tgaaaaaccc gaacagccat gggcgggaga
     1081 ctgccacata ccgcaccatt cggcaggaag ctggcgggca gtcgctgacg cgcagagcca
     1141 acctggcgcg ctggaccggg cgggtttgtc gagcgcgggg atttccggcc ggccctaccc
     1201 tttacagctc gcggaatccg ctgctagaag gccgagcaac atgatccgct cagtcctggc
     1261 accccgatct atccggcgtt tagcgctcac gacggcactc gtaatcgtcg ccgcttgttc
     1321 gcgccaaggc gaaggcgaac gctgcgattc gacggtcaat ggcaatgccg attgcgaagg
     1381 cgatttacaa tgcaaggcgc acggatcgtt ggccaccggc gacgtcgatc gctgctgccc
     1441 gccaggctcc ctgttcaacg acgtgcgttg cacccctggt tccggcgtct ccacgggcac
     1501 cggtggcaac acgaacggtt cgggcggtga caccagctcc gaaggcggcg ccacgtcgag
     1561 cgagagcgga ggaagcacct ccgcggcgtc tggcggggac acctcgtcta ccgcaagcgg
     1621 cggggcttcc accgcaacca ccgagacttc cggtgggcag atgtcccagg ccggcagctc
     1681 gagcgaaagc tgatacgtga cggaggtggt tcgagccgag cccacggttc atcctgcgca
     1741 gccgcttctg ttcccgagtg acgtgcggga cggtcgcacg cggattgcgg acgaagagca
     1801 gcgcatcgcg ggtcgtgtac tcctggtcca ggctgagacg ctggtcatcg cggatgcttt
     1861 tgcgtcgctt cgggtccgct gttcgacgca cctggaagtg aacgaaggcg actgggttat
     1921 cgtgcgcggg cagctcggct ccgatgagct gtccgcggcg cggctcgagt ttcatcagtc
     1981 ggcaccagct ccccacggct cgggcgaagt gggtcggttc gtattctcgg gcgtcggcac
     2041 tcacctggcg gcgcgagcgc gtgcgttgcg agaggtacgc gactacttcc aggatcgagg
     2101 tttcaccgag gtcgagacgc cgtggcgcgt tcccgcgccg ggtgtcgatc tgcacgtcga
     2161 agcttttcgc gctgacgcgg gttatctcat cacctccccc gaactcgaga tgaaacgact
     2221 gttggtgggg ggggtcccga agcaatttca gtttgcacgc gtcagccgtg ccgacgaagc
     2281 ggggcgtctc cacgaggccg agttcacgct gctcgagtgg tatcgagcgt tcaccagcta
     2341 cgaggcggtg caggaggata cagaagcgat cgtgcgccgc gtagctaccc ggattggcgc
     2401 gaatggtgcg cttacgacgc cgaccgggcg gcgcgttgcc gtggagcaga ccttcgagcg
     2461 catcacggta tccgaagcct tccgcactta tgccggcgtc acggatgtct ccgagctcgc
     2521 gcagcgagat gaagcccgtt acttcgagtt gctgatcgag caagtcgagc ccgcgctttc
     2581 gcgctgtgaa cgcccgattt ttttggtcga gtatcccatc tcccaagccg cgctcgcgca
     2641 gccttgcgct catgacgcgc gcttcgcgga gcggttcgag ctctacgccg gcggcgtcga
     2701 gctctgcaat ggctacggcg agctgacttg cccgaaggag caacgacgtc gcttcgaagc
     2761 ggaacagacg cggcgccgcg acggcggcct ccaggtgtac cccatcaatg agcgctttct
     2821 ttcggcgctc gccgagggca tgcccccgag cgcagggaac gcgctcggct tcgatcgctt
     2881 gctcatgctc gcgctgggcg ctcgacgcat tcaggacgtg atggcgttcc cgcgcgaacg
     2941 tgcctgagcc tctcggttga agcgcgctcc cggttcgatc gagagcgcgc ttcgccgata
     3001 gcgcgccccg tcagtcgaag cggacgatcg aaaccgtcgg cacgggcaaa agccgttctc
     3061 gcccgcccaa agacgagagc tcgatcacga acgcgtaggc caaaacgtac gcgccctggc
     3121 gatgcacgag ctcgccgcaa gcggcggccg tgctcccggt cgcgagcaaa tcgtcgacga
     3181 tgaccaccga cgcgccttcg cgcagcgagt cccgatgcat ttcgagctct gcctctccgt
     3241 actccatcga gtaggaaact ttgtccgttc gaaacggcag cttcccgggt ttccgcacgg
     3301 gcacgaagct cgcgttcaaa cgggcggcga gcgcgccgcc gaagatgaag ccgcgcgatt
     3361 ccacgccaac cactgcgtcc acttgcgacc ccacgaagcg atgcgcgatc gcgtcgagca
     3421 cgatgtgaaa gcccttcgga tcggcgagca gcggcgtgat atcgcgaaac aacaccgagg
     3481 gctccggaaa atccggaacc tcgcggatca acgcccgtac gtacgagagc gcatcgaact
     3541 cgtccggtaa cgagatctca tccagcttgt gacgctccgg cgccggtgct ggcttcttcg
     3601 gttccacgcc gcgtggcgtt ggcaacaagc gagcgggaac tggcttgctg cgtttgacga
     3661 tcgtgccttt tctcgttgcc atcagcggtc ctcgcgtcaa atcatgcgtt cagcactgcg
     3721 ccggaagcaa ccggctcggg tcagaagaag tgggtaccgc gacgtcacgc ggacggaacc
     3781 cgcttggcca gcgcctcggc gcgcgcgtcg aaggctttgc cccaaagatc caaaaccaga
     3841 ggtgtaatcg acaccgcccc cgcgtcgtac gcctcggtgt ccgagccc
//
LOCUS       DASTKU010000058        22280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_232789, whole genome shotgun sequence.
ACCESSION   DASTKU010000058 DASTKU010000000
VERSION     DASTKU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 22280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22280
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232789"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VFQ61_01935"
     CDS             <1..1007
                     /locus_tag="VFQ61_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9953230.1"
                     /translation="VFADRSLEPAEERRAAAARAYQASAGARGRLVRSSVDTYPPRCV
                     RARLRPDFSPISRVARGVLKRVEPLGGRSQTGTGASIQAQLGSLIMNTNIVERSRVLL
                     LEIGASPILYLMIALSIVSVGIMLERALFFFRSSEDLQRLAGELAARLGRGDVGSAKQ
                     LVGASRSAEAAVVSAGLNRIPQGSAAVEEAMSAARAIQKMRLERRLGFLGTLGANAPF
                     VGLLGTVIGIVQAFQHLEAGAASGGGTNGVMGAIAEALVATAVGLIVAIPAVVAFNSF
                     QRRIKGILGNAEALTHIVLSYVKATGSHGENSSSGPTTHVHSQSGDSGHLERLSTVPN
                     "
     gene            1047..1448
                     /locus_tag="VFQ61_01940"
     CDS             1047..1448
                     /locus_tag="VFQ61_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9953231.1"
                     /translation="MASAQTDDEEISGINVTPLVDVTLVLLIIFMVTAKLIAGQGIPL
                     DLPKAKTAGVVQTVLNVAVDSTGKINVNGRALESEQALRSEASAALRANPDLRTVISA
                     SSAASHGQVMKVLDTVRDAGVTRIAFAADKP"
     gene            1468..2295
                     /locus_tag="VFQ61_01945"
     CDS             1468..2295
                     /locus_tag="VFQ61_01945"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9953232.1"
                     /translation="MSTRASAPRLNPLVADLFPVSSSSGVRSSLGWLLAVSLHAGIFG
                     LALGRHAAQAKSERPPVEVEFVQPPPPAPPPEPEPLRPESPEPPTVAHAQPAKAPAAP
                     APAAARAGALHTAKADAAPPAADDPLDFTHDPNAMGFGGGVVAIGGTASFGAKGALPT
                     ANPAPPAPGQARRGTGEALTLAADLGRKPSLGESDPCRGYFPRNASDDSATASVLVVI
                     GSSGAVSKVTLLSENPSSQGFGAAARTCMQSKRFVPALDRAGRPTATAIRVNVRFTR"
     gene            2396..3937
                     /locus_tag="VFQ61_01950"
     CDS             2396..3937
                     /locus_tag="VFQ61_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9953233.1"
                     /translation="MSVSPSQARTSPSEIIGKMAGEYRLGRLLGEGGFGAVYEAEHPL
                     LKRRAAVKVLHRVADRDSDAVQRFVSEAQAVNQIRNRHIIDVFSFGTLADGRHFYVMD
                     YLEGEPLDRFLKRIGRLDVATALQLLQPIAEALDLAHGAGIIHRDLKPQNIFLAWDKN
                     DEVIPKLLDFGMAKLLSNSPVRTVSGTPIGTPLYMSPEQARGGQVDSRCDVYALGVLC
                     YEMLVGRVPFDGETTIAILMAHVIQAPERPSEVSDLPPEFDGPLLRMLEKDATRRPNS
                     AGEAIAALREVAQLTGTLVPAGLPKLPRPDPNAPVPPSGELEGEVASTLLANSPTPER
                     GTQVSLDHAANTTSRTRRRPWLAVAGVLMLGVGLTFWIRTTSTSKEASAVGSAQTPST
                     PPALIDTATATSAAAASSAATTTTAMSASPGPAQSASPAPDDPAAQAVPVERRVSTER
                     AVPPKRAVQLEATLVPPVIPSPAAISSGKRVKALTSPAKNPTSAEPPVGNPATPRDLE
                     SPFGP"
     gene            3996..6497
                     /locus_tag="VFQ61_01955"
     CDS             3996..6497
                     /locus_tag="VFQ61_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET9953234.1"
                     /translation="MSACTDLPEIESGECGNGVIEDGENCDTFARNPGGVCRPRGSVG
                     ECHLDCRRVNGKVSGSCPSGWGCDSDGLCREPSGNFDEDIKLPFAGVESLAAGDFDGD
                     GRADLVSREPTDSASQGKPTVHYFDPEGALVESLSFPKRVGTPYIGDISGDERSDFVF
                     TTFQIGVLLGRVDRTWVPETFSSYRLPNTHVRMASVIDRGVVSGDDGIAALATLGGET
                     GIFLPTQQGTLQLVVRLPEPVENLAEDFTTGDVVEGRWSPCREILLAFRGAETFSLYD
                     FCDTDPVLGIPSWRSTPHEQHVTFDPPARAERGPLLADLNGDGHLDVLIGASGRVYAA
                     YGDGQQLTTAVPYELQVQDAFWEGAEPPMPIAVGEFSGDRFPDFVFADHLLASLPGSG
                     VESNTPAYVVTQVNAGARWTEALVADFNGNGAADILATSRDGLNLSFYNGTGTPFMNA
                     ASLSTFGPVEHLALGDFDGDLLHDVAFIEHAASTEDTDSLSISFGAPFGPPSAPVTVA
                     HVKAAEQVMSFADSGLSSLIVASSRNHGGTLTGELALLLGSADRLPLALHTLVSLSED
                     GAVTSAPSVAISVGAFTGRNQHDLVSVAATETGSYEYWLIRSIDDNASAPIKLEGGVD
                     ERFGPFSAVTNGTPHVSTAGSAADLDGDGVDESIWILPAQSGSGCALFWFDVDVNRKR
                     TLPRGTLLLEGNCDQPSLMARDMDADGAPDLILLTSRSARDGASLSVLWNERGQFDLQ
                     RMTHFAGPPRVPQAFTVLPAFDTDETARLAFVTETSAEIAGVRGRDLGNSRTVRNLER
                     GTGIAGADFNGDGAVDLAIADAGKVVLLKGLFHAL"
     gene            6494..7699
                     /locus_tag="VFQ61_01960"
     CDS             6494..7699
                     /locus_tag="VFQ61_01960"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9953235.1"
                     /translation="MRQLSWAGVAATLLLAGVVQAQTPADLARSRDLFRAGASAYAAG
                     DYATAIQALEAAYELTPLPAIAFSLAQAERKQYLSSSRVQHLERAVSLFRRYLDEAPS
                     GTRRGDALEALKQLEPRLPEPQAHPEKPAPVRPTRLMVTVQAPGASVSVDGGPEAPSP
                     LIREVTPGQHRIRASAPGFTPAEREAIAVPGELILTELRLLERPVALTVVTPANAEVY
                     VDSEFAAQSSGKLELSLPPGEHDVVVNSKGHRSAKQRVRLVRGQPLRLRIKPELSTQR
                     VTSLALLCAAGASAGTGLVLSAFAIRSENNAETFLSARSQHNVTTASLAAYQASVNER
                     DRFRFASALSLASAAGLFITGLFLHELDRPRAPERRMAPRAEARLPSFGFVPVAPTGV
                     AGATLHVSF"
     gene            7744..8511
                     /locus_tag="VFQ61_01965"
     CDS             7744..8511
                     /locus_tag="VFQ61_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953236.1"
                     /translation="MSRLVCLWPLTLLFIATPALGQRDTLEQPYEADPNAPPPEAVQT
                     SEQRAPNNEAPAPMATLPHAAAVSGVVPEAPRADDRPLGPIRARRRLALTGELGWNGL
                     SGFGPVLSYHVMPELTVELGAGASFTGWKGGVRARYNFVKGAVTPFLGAGMMATGGLG
                     VVTGDFNDHASNPDAEGTDEVTIRVRPSQFVQVVGGLDWTASGGFTLIGTVGWASLLN
                     DNFEIVAGTPSREARQAFHAVFGSGPVIVLALGYSFR"
     gene            complement(8575..9570)
                     /locus_tag="VFQ61_01970"
     CDS             complement(8575..9570)
                     /locus_tag="VFQ61_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9953237.1"
                     /translation="MAVLVYGETGKISFSNPEACLLFAQGKPLDGENFLSLVPHAPEA
                     LRESLLHPTDTVFTVQAQAGLETFSLAKRTLDVTGEPHTLLMIKELTAELGRQEIEVW
                     KKVIRLINHELNNSLAPILSMVRTARFIATQPEQLPKLAGVFETIEERARHLAAFLEG
                     YARFARLPQPRPEPVVWAPFLDGMRALYPDAQIGEAPTREGYFDPGQLQQVVINLLKN
                     AFEASPKGAASVELRVDVDGAGATTLSVADRGAGMTAEVMQSAVLPFFTTKETGSGLG
                     LALAREIVEAHRGRLDISRRDGGGIEVKCWMPGRQTARDDLRSRARTTTLTLTHH"
     gene            complement(9791..11365)
                     /locus_tag="VFQ61_01975"
     CDS             complement(9791..11365)
                     /locus_tag="VFQ61_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9953238.1"
                     /translation="MSGLKVLLVDDQPAILAALSLLFDVRGIPTLVAKTPEQALELVR
                     SEEIGVVLQDMNFSRDATSGAEGESLLAAIRALDPDLPVVLMTAFQSLEAAVRLVRAG
                     ASDYIAKPWDDDKLLATVKNLMRLRELSRENLRFRSTGSRARAELAREHELCGLVYAS
                     SALHQVVSLAAKVAPSDAAVLITGPNGSGKELIAQIIQANSRRKKAPFVRVNAGGLPD
                     SLLEAELFGAEAGAYTGASKLRIGRFEEASGGTLFLDELGNLSMQGQMKLLRVLQTGE
                     FQRLGSNVTRKTDVRLISATNADLPAAIRAGSFREDLYFRLNVIELHIPPLRSRPDDI
                     LPIAELVLARHTKSTGQAFELSPDARRALVDHEWPGNVRELENRLQRATLVCEGPRIT
                     SADLGLEGTLGAALAASPVPATSSGTLPAPAAPRVSEARESLARESAPPESAPREAAV
                     PVVAGSPPPEPVPPEPPRVPEIEAQPTERAELERILVESGGVISTAAAMLGISRQALY
                     RRMDRLGITVERRVRG"
     gene            complement(11648..13921)
                     /locus_tag="VFQ61_01980"
     CDS             complement(11648..13921)
                     /locus_tag="VFQ61_01980"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9953239.1"
                     /translation="MSRISLRVYFVEDESGLLTGKLIARSSTRNEYLATGTTEEEVLA
                     RLGAQFDADSATEQLESFIWSEPLHVDRVVVEVRPQSSVGKRMVIGKQRIPIELHFVW
                     AALQGEQRDGTPAGFRVMLPRFGFAIVIEELRMAAEVLRQVVSAALTGESARSLFEFR
                     EVKREYVVDWSPRLRRARRTPAVQRESFDVLRSVAEDWCELAAAGKLGAHQGPANEAE
                     YLELIHARVKPSVLLVGPSGVGKTEWVKELARRAHRARTTELDVRVWATSADQILAGQ
                     VYLGMWEQRCLDMIAELSGEGHYLFLDRLGDFVRPRSGASAISDLFLTALTENTLSVI
                     AECTPEEFQRLQSRHASLLSRFRVIRLEPPEREEACQRAAEYAARALPGWRIPSESVR
                     RLVRHLQSFKKSQAFPGSAFGFLGWLVQHESSSAQTPGAKSLEPPEIDGLFSRYTGLG
                     AQLISEGERAPASRIALALQQRVAGQNRACDGAAQVLARFKAGINDPEKPIGSLLFVG
                     PTGVGKTELAKELARYLFGSEERLVRLDMSEFMLANSSARLLADERGAASLVQRVGEQ
                     PLSLILLDEIEKAHSAVFDLLLGLLGEGRLTSASGRLVDFRMTLVVMTSNLGTDVSTL
                     GFGDSSASAQPYVGAVRAHFRPEFLNRIDQIVPFSALTPEALRKIVEFLLAELVEREG
                     LKRRNLRLQVSDRAKDQLALLGTNRRYGARPLKRVIEQQVVTPLAIELARRPSLQDAR
                     VHVTCSDAGIEVQIQAP"
     gene            complement(13918..17493)
                     /locus_tag="VFQ61_01985"
     CDS             complement(13918..17493)
                     /locus_tag="VFQ61_01985"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9953240.1"
                     /translation="MQIAISVYQQKQGNSYALTPVVPGLFNWTVQSGDLGKARQRLIE
                     RVRREATKCERAAFRRCIVPAGRYLNRLHLQLVLRHQGEKRRLTGHFPVVIEERDAGT
                     AGRMRIAYHPLRSSDWFELSEGEELVTDVGRRYSRIFEADDELETLKCSGNEKLFTLR
                     FHVDAPSLRTLLKDTKREEGLAASGVSGQLEALMRVTVNQTSRAASGLLRLGLPRPRE
                     RRELQRLLCGPKKVSVLVVGPPGAGKSALLAQAVQDLLDADEYPTHGNLDRVHNVLSL
                     SGKQIIAGMSYVGQWEARCVELLARCKRRKLVLWIDDLPAFASLGRTTVSERVLADFF
                     RGPVARGDLVMLGECSPEQHRALEYEAPGFASAFETLLLSEATPEESLAMLLFESRRL
                     EQELDIAFEPRALRAILEQSGTLYGGTALPGRALAPLKAIARLHAQAGKTEQSMDPSQ
                     SVAVESIGSPEVLEFFSTQTGLPKLLLTADAPLTRDELARQLARHVLGQAQAIEAACD
                     LILSLRAQLVGPGRPYSVVLFTGPTGTGKTELAKCLAEYLFGAQERLLRFDMGEFNGP
                     DAVARLIGDAIEPDGLLTSSVRSQPFAVLLFDEIEKAHPTVLNLMLQIFEDARLGDAR
                     GNVTDFRHTLIVMTSNLGTGRSTVRGFAEDANAAAAHVAHAVREFFPPELFNRIDRIV
                     PFSTLDEQAAREIVRKELSSLVARPGLGERNVFVRFTEAVTERVTREGYDPEFGARAL
                     KRHIEREVGGVLTEALTQDHPAEMRLLWMHARESGQGVGVRVEALREAAPHARSSPVQ
                     HLLECDYTELCSYVPAALTRLAAFERDGRLPELGQRMSRELSQFRLGDRSRADQVFNL
                     DALLSHAERLRQRLEARISGDSELQAADRRQKVARGEALTEQDFSILGADAGRRFARD
                     PRRPRSGEFTPSGPTHLGQASSLGRSQALLQELVEVALLEKLLDTAHEGGRHVVLIEV
                     LQLSLHHERRRFSKAGSGLGSWLASSYAKARGVLDGVAVMTWDGDIQTVPVGGLDEAL
                     QNRVKLAVLRLVGPGVFDFFRMETGCHVRRTTAAGSEIVRVRVLPGITSHPPDASNAA
                     ALEPRVWIREHVDRVKAFERGLEARGPLIENPEQLLPVVRTLRFEPDPEGYSPAYVED
                     YRFAHVTSRRVRTLSEVLSDFWLLGAALDSSLLSDQAQPASEVAP"
     gene            complement(17544..20966)
                     /locus_tag="VFQ61_01990"
     CDS             complement(17544..20966)
                     /locus_tag="VFQ61_01990"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9953241.1"
                     /translation="MASLVLRPLVVSCAYAEGLCAAFPASAPHFIAYGASSEEAESEL
                     IDFLGDHFERLEPDQLQSFLVSETAALRHVSVIIPRADLPRAERIRTALQVPIVEVAA
                     PAGAKWLLIPSVGQVSYVAASSAQDVEDAVREEVLRLAAAADIDGASYLDLFPPAQIE
                     LSRPLVEIDPEQRLERKAGKSSATLRQRRAAERLLERVGRLVSKPAYSVASRPMEELQ
                     GLVSGKGRRSLALVGPEGVGKSDWFRAAVSQVGRVVYATSGAEIVAGQAGLGQLQERF
                     DAVMSAAELLEATLYFDSFEDLFAGREGGHEDMTALLRRHLQQGRVQIVAELSAEAYE
                     RLGQRYAGIFSYLHRFNVAPLTREQTLTILQERVRADQRRGELALSSAALSQILDLAD
                     RYEPYRVLPGKAVRLYEELRARARDAASSDPSGAVTQLSPAQVLAAFASKTGIPEFLL
                     RDDQNLHVADVRRFLQQFVIGQDHAVQRLAELLCTIKARLQPAGKPLATLLFLGPTGV
                     GKTELAKALARFLFGSPERMARFDMSEYMDAYAADRLIRGSERADGALTQRVREQPFG
                     VLLLDEIEKAHPNVFDLLLQVAGEGRLSDARGKVAYFHNTILILTSNLGARERRARVG
                     FGSGGQEESASYYQRQVERHFRPELVGRLDGIIGFTSLDSEQIIQVARLFVDRIGRRA
                     GLEARGAQLLISEAALLQLAQGGFSPEYGARGLRRYLEQVLVAPLSGVISALGAQAQG
                     AKLLVCSRAEHDANASEGRATRPDGWSASLEDHYPSDLAPAVSEVIVISAALARQRER
                     AQSESGINSVSRMRRSMRRWNALQPLSEVRERGAELTVLLAEVARTSGRAAERSTLQA
                     QRMSELSREHANVRGWLDPLDEALREIEEIEGLLLSAIDETPPDLEPEASLAYQRFRG
                     QLLWVLLQSSQQNTVTLAIHELDDRRLLHLLLLPWLHFAQRQNWEVVLHFDRVARDGD
                     WPTLEQRRWGPPIAMNTYLTKVPEPRPADAVLLRMRGAGAAAFAAFYLGRYRRPDREG
                     VGELWVRYLRPRFEVRAEDWEHSDFRASIDRSVGRGQPLLAEFAEASASRSAASPALA
                     ANSSAAGVTVSPSELFDSMTDFLFERVLERVQSGKPFLPPSRFDRASPDELA"
     gene            21663..>22280
                     /locus_tag="VFQ61_01995"
     CDS             21663..>22280
                     /locus_tag="VFQ61_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953242.1"
                     /translation="MRTFFSLIALPITFVVACSDSPASGVSSGTGGGLSPQGGSGNAG
                     TANTANGGTPGSASGGTGTTSMANGGALNSGGSPMASGGAAITSNGGASNGGASTSRG
                     GASAGGQTSSAGATNSSGGSSGGVSPSSGGGGAGGTSGAASGGKGGEASTGGSGGGNP
                     GGCTRELLKSAVDAYFKALAAHDSSTLPVAEGVKFTENGKSMKLGE"
BASE COUNT         4012 a         7495 c         6977 g         3796 t
ORIGIN      
        1 aggtattcgc cgatcgatcc ctcgaaccag cagaggagcg aagagcggcc gcggcgcgcg
       61 cgtaccaggc gtccgccggg gctcgcggac gcttggtgcg aagctccgtg gacacctatc
      121 cgccgcgctg tgtccgcgct cggcttcgtc cagattttag cccgatttcg cgcgtggcac
      181 gcggcgtgct gaagcgggtc gagccgctcg gcgggcgctc gcaaacgggc accggcgcga
      241 gcatccaagc gcagctcggg agtctcatca tgaacaccaa catcgtcgaa cgttctaggg
      301 tcttgttgct cgaaattgga gcctcaccca ttctgtatct catgatcgcg ctgagtatcg
      361 tgagcgtggg gatcatgctg gagcgcgcgt tgttcttctt ccgcagcagt gaagatctgc
      421 aacggctcgc cggcgagctc gcggcgcgac tcggccgagg cgacgtgggc agcgccaagc
      481 agctggtcgg agcctctcgc tcggccgaag cggcggtggt cagcgccggc ttgaaccgaa
      541 ttccgcaagg ctccgccgca gtggaagagg ccatgtcggc cgcacgcgcg attcagaaga
      601 tgcggctcga gcgcaggctc gggttcttgg gcacgctcgg ggccaatgct ccgttcgtcg
      661 gtctactcgg cacggtcatc ggcatcgtgc aggcgtttca gcacctcgag gcaggcgcgg
      721 cgtccggagg cggcaccaac ggcgtgatgg gcgcgatcgc agaagcgttg gtggccaccg
      781 cggtgggtct gatcgtcgcg atcccggccg tggttgcgtt caactccttt cagcgacgga
      841 tcaagggcat tctgggcaac gccgaagcgc tcactcatat cgtgctctct tacgtgaaag
      901 ccactggctc gcacggagag aactcgagtt ccgggccgac cacgcacgta cactcgcaaa
      961 gcggggacag cgggcatctc gagcggttga gcaccgtccc caactgacga atcccacgtc
     1021 gaccataact cacgaggacc cgaccgatgg ccagtgcaca aaccgacgac gaggaaatct
     1081 cgggcatcaa cgtcactcca ctcgtggacg tgaccctggt gctgctcatc atcttcatgg
     1141 tcaccgcgaa gctgatcgcg gggcaaggca tccccctcga tcttccgaag gcgaagaccg
     1201 cgggcgtggt gcaaacggtc ttgaatgttg cggtggattc aacgggtaag atcaacgtaa
     1261 acggacgcgc gctggagagc gaacaagcgc ttcgctccga ggcgagcgcc gcgctgcgcg
     1321 ccaaccccga ccttcgcacg gtaatttcgg cttcttccgc ggcgagccac ggccaagtca
     1381 tgaaggtgct cgacaccgtg cgcgacgccg gcgtgacgcg tatcgccttt gccgccgaca
     1441 agccttgaac ctcgcaggag ctttgccatg tctacccgcg cttcagcccc aaggctgaat
     1501 cccctcgttg ccgatctgtt ccccgtctcg agctcttctg gtgtccggag ttcgctcggg
     1561 tggctgctcg ccgtctcgct tcacgcaggg attttcggtc tggctctcgg acggcacgcc
     1621 gcgcaggcca agagcgaacg cccacccgtc gaggtggagt tcgtgcaacc accgcctcct
     1681 gcccctcccc cggagccaga accattgcgt ccggagtcac ccgaaccgcc aaccgtcgct
     1741 cacgcccagc ccgccaaagc gcccgccgca cccgcacccg ctgcagctcg tgcgggggcg
     1801 cttcataccg ccaaggcgga cgcagctccc cccgccgctg atgatccgct cgactttacg
     1861 cacgacccca acgcaatggg ctttggcggg ggcgtggtcg cgatcggcgg aacggcgagt
     1921 ttcggagcca agggtgccct cccgacggcc aaccctgcac cgccggcccc gggtcaggct
     1981 cgccgcggca cgggtgaggc gctcacgctc gccgcggacc tcggtcgcaa gccgagtctg
     2041 ggtgagtcgg acccctgccg gggctacttt ccgcgtaacg ccagcgacga ctccgcgacc
     2101 gcgtcggtcc tggtcgtgat cggcagttca ggagcagtct cgaaagtgac cttgctaagc
     2161 gagaacccga gttcacaagg tttcggagcg gcggcgcgca cgtgcatgca aagcaagcgc
     2221 ttcgtaccgg ccctcgaccg cgctgggcgc cccaccgcca ccgcgattcg cgtaaacgtc
     2281 cggttcacgc gctgagcaaa ggggcgccca agttggcaca gcccggtcca ctcgaaaccg
     2341 caggcggagc gcggacccgg ttgcgtgcca ggccgctccg tgtgatatcg acgacgtgtc
     2401 ggtttcgccg tctcaggctc ggacctcgcc ctccgaaatc atcgggaaaa tggcgggtga
     2461 ataccggctt ggccggctcc tgggggaagg cggattcggg gcggtgtacg aggccgagca
     2521 ccccttactc aagcgccgcg ccgccgtgaa ggtactgcac cgtgtcgcgg atcgggactc
     2581 ggacgccgtg cagcgcttcg tgagcgaagc acaggccgtc aatcagattc gtaaccgcca
     2641 catcatcgac gtgttctcgt tcggaacgtt ggcggacggg cgtcacttct acgtgatgga
     2701 ttacctcgag ggggagccgc tcgatcgctt tctgaaacgc atcgggcgtt tggacgtggc
     2761 gaccgcgctg cagctgctgc aaccgatcgc ggaggcgctc gacctggctc acggagcggg
     2821 catcatccat cgtgatttga agccacaaaa catcttcttg gcttgggaca agaacgatga
     2881 ggtgatcccg aagttgctcg acttcggcat ggccaagtta ctctccaact cgccggtgcg
     2941 caccgtgagc ggcacgccga ttggaacgcc gctctacatg tcgccggagc aggcgcgcgg
     3001 tggacaggtc gactcgcgtt gcgacgtgta cgcgctcggc gtgctttgtt acgaaatgct
     3061 cgtgggccgc gtgcccttcg acggagagac cacgatcgca atcttgatgg cgcacgtcat
     3121 ccaagctccg gagcggccct ccgaagtcag tgacttgccg cccgagttcg atggcccctt
     3181 gctgcgcatg ctcgagaagg atgcgacccg acgcccgaac tccgccggcg aagcaatcgc
     3241 cgcgctgcgc gaagtggcgc agctcacggg cacgctggtt ccagcgggcc tacccaagct
     3301 gcctcggccc gatcccaacg cgcccgtgcc tccatctggt gagttggagg gagaggtcgc
     3361 atcgacctta ctcgcgaact ctccaactcc cgaacgtggc acccaggtct ccctggatca
     3421 cgccgcgaac accacgtcga gaacacggcg caggccctgg cttgcggtgg ccggtgtttt
     3481 gatgttgggg gtcgggctga cgttctggat tcgcacgacg agcacttcga aagaggcgtc
     3541 cgcggtaggc tccgcgcaga ccccgagcac accgcccgcg ctgatcgaca ccgcgacggc
     3601 aacttcggca gccgcggcct cttcagcagc aacgaccacg accgcaatgt ctgcgtcgcc
     3661 cggtcccgcc cagtcggcct cgccagcgcc cgacgatccg gcagcgcaag cggtaccggt
     3721 cgagcgccgt gtttcgaccg agagagctgt tccgcccaaa cgcgccgtac agctcgaagc
     3781 cacgctcgtt cctccggtca ttccgagccc cgccgcgatc agctccggca aacgggtgaa
     3841 ggcgctcacg agccccgcca agaatcccac gagtgccgag ccgcccgtcg gcaatcccgc
     3901 gacccctcga gatctggaga gcccgtttgg tccgtgattc tggcccacgc ctgcgacggg
     3961 cgcgtgcgtt atgcgcgatt tggttaacgc tcggagtgag cgcctgtacg gacttgcccg
     4021 aaatcgagag cggcgagtgc gggaacggtg tgatcgagga cggcgagaat tgcgatacct
     4081 tcgccagaaa tccgggaggc gtgtgccgcc cccgaggcag cgtcggggaa tgtcacctcg
     4141 attgtcgacg ggtgaacggt aaagtctcgg gctcttgtcc tagcggttgg ggctgtgatt
     4201 cggatggctt gtgccgcgag ccaagcggca acttcgacga agacatcaag ttacccttcg
     4261 cgggtgtgga gtcgctcgcg gccggagact tcgatgggga tggccgcgcc gatctcgtga
     4321 gccgagaacc tactgactcg gcgtcacaag gcaaacccac ggtgcactac ttcgatccgg
     4381 agggcgcgct ggtcgagtcg ctttccttcc cgaagcgagt gggcacacct tacatcgggg
     4441 atatctcggg ggacgagcgc tcggatttcg tgttcacgac gttccagatc ggcgtgttgc
     4501 tgggccgcgt cgatcgcacc tgggtgccag agaccttcag ctcttatcgc ctgcccaaca
     4561 ctcacgtgcg aatggcgtcg gtcatcgatc gaggcgtggt ttcgggtgat gacggcatcg
     4621 ccgcgttagc gacgttgggc ggagaaaccg gcatttttct gccgacgcag cagggcaccc
     4681 tgcagctcgt cgtacggcta cccgaaccgg tcgagaacct cgccgaagac ttcacgaccg
     4741 gcgacgtcgt cgaaggtcgg tggtctcctt gccgggagat cttgctcgcg tttcgaggag
     4801 cagagacctt ttccctgtac gacttttgtg acaccgatcc ggtgctcggt attccgagct
     4861 ggaggagcac gccgcacgag caacacgtga ccttcgatcc gccggctcgt gccgagcgcg
     4921 gtccgctgtt ggcggacttg aacggcgacg gtcatttgga cgttttaatt ggcgcctcgg
     4981 gtagagtcta cgccgcgtac ggcgacggcc agcaactgac gacggccgtg ccgtacgagc
     5041 tccaggtgca ggatgcattc tgggaaggcg cggagccgcc catgcctatc gcggtcggag
     5101 agttcagcgg cgatcggttc ccggacttcg tattcgcaga tcaccttttg gcatccctgc
     5161 ctggtagtgg tgtggagtcg aacactcccg cgtatgtggt gacacaagtg aacgcgggcg
     5221 cgcgctggac ggaggccctg gtggccgact tcaacggcaa cggcgccgcc gatatcctcg
     5281 cgacttctcg ggacgggctg aacctgagct tctacaacgg caccggcacg ccattcatga
     5341 acgcggccag cctctcgacg ttcggtcccg tggaacactt ggcgctcgga gatttcgacg
     5401 gagatctcct gcacgacgtc gcgttcatcg agcacgccgc gagcacggaa gatacagatt
     5461 cgctctcgat ctccttcggc gcgccgttcg ggcccccgtc cgcacccgtc acggtggcgc
     5521 acgtgaaggc ggccgaacag gtgatgagct tcgccgactc cggcttatcg agcttgatcg
     5581 tcgcctccag tagaaatcac gggggcaccc tgaccggtga actcgccttg ttgctgggca
     5641 gcgcggatcg actgcccttg gccctgcaca ctctggtcag cttgagcgag gatggcgcgg
     5701 tgacctcggc cccctcggtc gcgatctcgg tgggagcatt caccgggcgc aatcaacacg
     5761 atctggtgag cgtcgctgca accgagactg gaagctacga gtattggctc atccgctcga
     5821 tcgatgacaa cgcttcggca ccaatcaagc tcgagggcgg cgtagatgaa cgcttcgggc
     5881 cgttcagcgc ggtcacgaac ggcacaccgc acgtctcgac cgccggcagc gcggctgatc
     5941 tcgatggcga tggggtcgac gaatcaatct ggatcttgcc ggcgcaaagc ggctctggct
     6001 gcgcgttatt ttggttcgat gtcgacgtga accgcaaacg tacgttgccg cgcggcacgc
     6061 tcttactcga gggcaactgc gaccagccgt cgctgatggc ccgagacatg gatgcagacg
     6121 gagcgccgga tctgatttta ctcacctccc gttcggcgcg agacggcgcg tcgctctccg
     6181 tgctttggaa cgaacgaggc cagttcgatt tgcagcgcat gacccacttc gcggggccac
     6241 ctcgggtgcc ccaagcgttc acggtgctgc cggccttcga cacagacgag acggcacgcc
     6301 tggctttcgt gaccgagacc tccgccgaga tcgcgggagt acgaggacga gatctaggca
     6361 attcgaggac cgtacggaac ttggagcgag gcaccgggat cgcaggggct gacttcaacg
     6421 gggatggcgc ggtcgatctc gcaatcgcag acgccggtaa ggtcgtgctc ctgaaaggac
     6481 tgtttcacgc actatgaggc aactctcatg ggctggtgtg gccgcgacgc tactgctggc
     6541 gggagtagtc caagcgcaga cgcccgccga tctagcgcgc tcacgggatt tgttccgcgc
     6601 gggcgcgagc gcctatgcgg cgggtgacta cgcgacggcg attcaggcgc tggaggccgc
     6661 ttacgagctc acgccgctgc cggcgatcgc gttttctctc gcgcaggccg agcgcaaaca
     6721 gtacctgagc tccagtcgcg ttcagcacct ggagcgtgcg gtgagcctat tccgccgtta
     6781 cctggacgaa gccccctctg gaacgcgccg aggcgatgcg ctcgaggcgc tgaagcaact
     6841 cgagccgcgt ttgccggagc cgcaagcgca cccagagaag cccgcgccgg tgcggcctac
     6901 tcggctgatg gtgaccgtcc aggcgccggg cgcgagtgtc tcggtggatg gtgggcccga
     6961 agcaccgtcc ccgctgattc gcgaggtgac tcccggtcaa caccgcattc gcgccagcgc
     7021 tccggggttt acgcccgccg aacgcgaagc gatcgccgtc cccggcgagc tgatcttgac
     7081 cgagctgcgc ctcctcgagc gaccggtggc gttgacggtg gtcacccccg ccaacgccga
     7141 agtgtacgtg gatagcgaat ttgcggccca gagctccggc aaactcgagc tctccttgcc
     7201 gcccggagaa cacgacgtcg tcgtcaactc caagggtcat cgcagcgcga agcagcgcgt
     7261 tcgcctggta cgcggccagc ccctccgact ccgcatcaag cccgagctct ccacccaacg
     7321 ggtgacttcg ttggccttgc tgtgcgcggc cggcgcgagc gccgggactg ggctcgtgtt
     7381 gagcgcgttc gcgatacgtt cggagaacaa cgcggagacc ttcttgagtg cgcgttctca
     7441 acacaacgtg accaccgctt cactcgcggc ttaccaagct tcggtgaacg agcgagatcg
     7501 atttcgattc gcgtccgcgc tcagcctcgc gagcgctgcc ggcttgttca tcacagggct
     7561 atttctgcac gagctggatc ggccacgcgc gccggaacgg cgcatggcgc cccgtgcaga
     7621 agctcggcta cccagcttcg ggttcgtgcc cgtcgctcca accggagtcg ccggcgcgac
     7681 tcttcacgtt tcgttctgag attggcgcga ctcatgctta tcccgttccc catgcctgtc
     7741 cgaatgagcc gcctcgtttg cctttggccg ctgacgctgc tcttcatcgc aacgccggcc
     7801 ctaggccaac gagatacgct cgaacagccc tacgaagcgg atcccaacgc gccgcccccg
     7861 gaagctgttc aaacgtccga acaacgcgct ccaaacaacg aggcgcccgc cccgatggcc
     7921 acgctccctc acgccgccgc agtgtccggc gtcgttccgg aagcgccgcg cgcggacgat
     7981 cgtccgctcg gacccattcg agcgcgtcgc cgcttggcgc ttacaggaga gctgggttgg
     8041 aacgggctct cgggcttcgg cccagtgctg agttaccacg tcatgcccga gctcaccgtg
     8101 gagctcggcg cgggtgcctc gttcactggc tggaaagggg gcgtccgcgc gcgctacaac
     8161 ttcgtaaagg gcgcagtcac cccgttccta ggcgccggca tgatggcgac gggcggtttg
     8221 ggtgtagtga ccggtgactt caatgatcac gcttcgaacc cggacgccga gggcaccgac
     8281 gaggtcacga tccgcgtgcg tccgtctcag ttcgtgcagg tggtgggagg cctggattgg
     8341 acggccagcg gcggttttac gctgatcggt acggtcggtt gggcctcgct gttgaacgac
     8401 aatttcgaaa tcgtggccgg cactccatcc cgtgaggccc ggcaagcttt ccatgccgtt
     8461 ttcggctctg gccctgtgat cgtgctcgcg ctgggttact cgttccgttg agcggtggcg
     8521 cttcgcgggc ggacaggtgt ccgttggccg atctcggacg gcgaggtgcg aagctcagtg
     8581 gtgggtgagc gtcagggtcg tggtgcgagc ccgcgagcgt aggtcatcac gggccgtctg
     8641 cctgcccggc atccaacact tcacttcgat accgcccccg tcgcgccgag aaatgtccaa
     8701 gcggccgcgg tgcgcctcca cgatttcgcg agcgagtgcg agaccgagac cactgccggt
     8761 ctccttggtc gtgaagaacg gcagcacggc gctctgcatg acctcggccg tcatccctgc
     8821 accgcgatcg gccacgctga gcgtcgtggc gccggccccg tcgacgtcga cccgcaactc
     8881 tactgacgct gcgcctttgg ggctcgcttc gaaggcgttt ttcagcaagt tgatcacgac
     8941 ttgctgcaac tggccggggt cgaagtagcc ttctcgtgtg ggtgcctcac cgatctgcgc
     9001 gtcgggatag agcgcccgca tgccgtcaag gaacggggcc caaaccactg gctccggtcg
     9061 tggctgcggc aagcgcgcga aacgcgcgta gccctcgaga aaggccgcta ggtggcgcgc
     9121 acgctcttcg atggtctcga acacgcccgc gagcttcggc agctgttcgg gttgagtcgc
     9181 gatgaagcgc gcggtgcgca ccatcgatag gatcggagcc agcgagttgt tgagctcatg
     9241 attgatgagg cgaattacct tcttccagac ttcgatttcc tgacgcccga gctcggcggt
     9301 cagctccttg atcatcaata gtgtatgtgg ctctcccgtc acgtcgagcg tgcgcttcgc
     9361 gaggctgaat gtctctagcc ctgcctgcgc ttgcaccgta aacacagtgt cggttgggtg
     9421 caggagactc tcacgcagcg cctccggcgc gtgcggcacc aggctcaaga agttctcgcc
     9481 gtcgagcggc ttaccttgcg cgaaaagcag gcacgcttcg ggattggaaa aggagatctt
     9541 gcccgtctcc ccgtacacca acacggccat cggcgcgtgc tcgataatcg cgcggagtaa
     9601 cgttaggtgt ccttcacttg gcgcacggag gccagactcc agggcttggc tcgaaccgaa
     9661 cgcgggcgga ggagcgtggg acggtcgttt ctccgattca acccttcgcc cgagctttct
     9721 gccggaaacg aacccgagcg caaggagcag accggccagg acggtggcga gttcagccga
     9781 gagttgcaac tcaacctcgc actcggcgct cgaccgtgat gcccaagcgg tccatcctcc
     9841 gatagagcgc ttgccgacta atccctagca tggctgcggc cgtcgaaatc acgccgccgc
     9901 tctcgaccag gatgcgctcg agctcggcac gctcggtggg ttgagcttcg atttccggaa
     9961 cccgcggcgg ttcagggggc accggctcag gcggcgggga ccccgccacg actggaacgg
    10021 cagcttcacg gggagcggac tcgggtggcg cagactcacg cgccaacgac tcccgggctt
    10081 cggacacgcg cggcgcggct ggggccggca gggtgccgga cgaggtagcc gggaccggcg
    10141 aggccgcgag cgcggcgcca agcgttccct cgagccccaa gtcggcgctc gtgatgcgcg
    10201 gtccttcaca aaccagggtg gcgcgctgca agcggttctc gagctcacgc acgttgccgg
    10261 gccattcgtg atccaccagc gcgcgtcgcg cgtccggcga aagctcgaaa gcctggcccg
    10321 tgctcttggt gtgacgggcg agcacgagct ctgcaatcgg caaaatatcg tccggtcgcg
    10381 aacggagcgg cgggatgtgc aactcgatca cgttcaaccg gaagtagaga tcttctcgga
    10441 agctcccggc gcggatcgcc gcgggcaaat cggcgttggt cgcgctgatc agtcgcacgt
    10501 cggttttgcg ggtcacgtta ctgcccaggc gctgaaattc gccggtttgc agcacgcgca
    10561 agagtttcat ctgaccctgc atcgagaggt tgccgagctc gtcgaggaac aacgtcccgc
    10621 cgctcgcctc ctcgaagcgg ccaattcgca gcttgctcgc gccggtgtac gctcccgcct
    10681 cggcgccaaa gagctcggcc tcgagcaagg agtcgggcaa accgcctgca ttgacgcgca
    10741 cgaagggcgc cttcttgcgc cgggagttgg cctgaatgat ttgggcaatc agctctttgc
    10801 ccgagccgtt ggggcccgtg atgagcaccg ccgcgtcgga gggcgcgacc ttggccgcta
    10861 gcgacaccac ttgatgcaac gccgagctcg cgtagacgag cccgcacaac tcgtgctccc
    10921 gcgccaactc cgcccgggcc ctcgagcccg tcgatcggaa acgcaggttt tcgcgcgaca
    10981 gctcgcgcaa gcgcatcagg ttcttcacgg tcgccagcaa cttgtcgtcg tcccaaggct
    11041 tcgcgatgta atcactcgcc cctgcccgca cgagccgcac cgctgcttcc agagactgga
    11101 aggcagtcat caacaccacc ggcaggtccg gatccagcgc ccgtatcgca gccaacaggg
    11161 actcaccttc ggcgccgctc gtcgcatcac ggctgaagtt catgtcttgc agcacgacgc
    11221 cgatttcttc cgagcgcacc agctcgagcg cctgctccgg ggtcttggcg acgagcgtcg
    11281 gaatgccacg cacgtcgaac agcaagctga gcgcagccag tatcgccggc tgatcatcga
    11341 ccaacaaaac tttcaagcca ctcatgatgc tcatcgcccg ccgcggggcc tcgctcgaac
    11401 ctttctcaca agattcgtga acctatccca agccttcgcc gggggccatt cgcccgacag
    11461 atccaaggcg tagggggtca ccaaaagctg acctttcccg ggcaagtcgc gccgcgggac
    11521 ccgagtaaag ctccgagctc gagtcaggat tttcgcctcc gcgtcgttga tcgccgtcag
    11581 ctatcgagtt cgcgtcgacc aaaacagagg tgcccgtcta ctactcgtgg agttcacgaa
    11641 tcgtggctca cggcgcctgg atctgcactt cgatgcctgc gtccgagcac gtcacgtgga
    11701 cgcgcgcatc ctgaagtgac gggcggcgcg cgagctcgat cgcgagcgga gtcactacct
    11761 gctgttcgat gacgcgtttc aagggccgcg cgccgtagcg gcgattggtg ccgagcagcg
    11821 ccagctggtc tttcgctcga tcgctgacct gtagccgcag gtttcggcgc ttcagccctt
    11881 ctctttcgac cagctcggcc agcaagaatt ccacgatttt gcggagcgcc tccggtgtga
    11941 gcgccgagaa cggcacgatc tgatcgatcc gattcagaaa ctccgggcgg aaatgagcgc
    12001 gcaccgcgcc gacgtacggc tgcgcgctgg cactcgagtc tccgaagcca agagtcgaaa
    12061 cgtccgtgcc caggttgctg gtcatcacca cgagcgtcat ccggaaatcg accaagcgcc
    12121 cgctcgcact cgtcagtcgc ccttcgccga gcaagcccaa aagcaagtcg aacaccgccg
    12181 agtgagcctt ctcgatctcg tccaacaaga tcaagctcag cggctgctca cccacgcgct
    12241 gaacgaggct cgcggcgcca cgctcgtccg cgagcagccg cgcgctcgag ttcgcgagca
    12301 tgaactcgct catgtctagg cgcacgagcc gttcctcact cccgaacaga tatcgtgcga
    12361 gttccttggc gagctcggtc ttgcctaccc ccgtggggcc cacgaagagc aggctcccga
    12421 tcggcttctc cgggtcgttg atgccggcct tgaagcgcgc gagcacctgc gcggctccat
    12481 cacacgctcg gttctggccc gccactcgtt gctgaagcgc cagcgcaatc cgcgacgccg
    12541 gagctcgctc gccttcgctg atcagctgcg ccccaaggcc cgtgtagcgg ctgaagaggc
    12601 catcgatttc gggcggctcg agtgatttgg cacccggcgt ttgcgcgctc gaactctcgt
    12661 gctgcacgag ccagcccaag aacccgaacg cgctccccgg aaaggcctgg gacttcttga
    12721 acgattgcag atgacgaacc agccgacgca ccgactccga tgggattcgc caaccgggca
    12781 acgctcgcgc ggcatactcg gccgcccgct ggcaagcctc ctcccgctcc ggcggctcca
    12841 gacgaatcac ccggaagcgt gacaagaggc ttgcgtgacg cgactgcagc cgctgaaact
    12901 cctcgggggt gcattccgcg atcacgctca acgtgttctc ggtcagcgca gtcagaaaca
    12961 ggtccgatat ggccgaagcc cccgagcgag ggcgaacgaa atcacccagg cgatcgagga
    13021 acaaatagtg cccctcgccg ctcagctcgg cgatcatgtc caggcagcgc tgctcccaca
    13081 tccccagata cacctgaccg gccaagatct ggtcggcgga cgtggcccag acccggacat
    13141 cgagctccgt cgtacgggcc cgatgggccc gacgcgcgag ctccttgacc cactcggttt
    13201 tgccgacgcc actcggccca accaagagca ccgagggttt gacgcgagcg tggatcaact
    13261 ccagatactc ggcttcgttt gccgggcctt ggtgagcacc gagcttgccc gcggccgcga
    13321 gctcacacca gtcttccgcc acactgcgca acacgtcgaa gctctcacgt tgcacggcag
    13381 gggtgcgacg tgctcgccgc aggcgcggag accaatcgac cacgtactcc cgcttgacct
    13441 cgcggaactc gaacagggac cgtgctgact cacccgtcag cgctgcgctc accacctgac
    13501 gcagtacctc ggcggccatg cgcagctctt cgatcacgat cgcaaacccg aagcgcggca
    13561 gcatgacccg aaaacccgcg ggcgtaccgt ctcgctgttc cccctgcagc gcggcccaga
    13621 cgaagtggag ctcgatcggg atccgctgct tgccgatcac catgcgcttc cccaccgagg
    13681 attggggccg cacctccacg acgacccgat ccacgtgcag gggttcggac cagatgaacg
    13741 actcgagctg ttcggtcgcc gaatccgcgt cgaactgcgc gcccaagcgc gccaaaacct
    13801 cttcttcggt cgtgcccgtg gccaggtact cgtttcgcgt ggagctgcgg gcgatcagct
    13861 tacccgtaag caggccgctc tcgtcctcca cgaagtacac acgcagcgag atgcggctca
    13921 tggcgccacc tccgaggccg gttgcgcctg gtcgctcagc agcgagctat cgagcgcggc
    13981 gcccagcaac cagaagtcgc tcaacacctc actcagcgtg cgtacgcgac ggctcgtgac
    14041 gtgcgcgaag cgataatcct cgacgtaagc gggcgagtat ccctccgggt cgggctcgaa
    14101 gcgcagcgtt cgcacgaccg gcagcagctg ttcgggattc tcgatcagtg gcccgcgcgc
    14161 ttccaagcca cgctcgaacg ccttcacgcg atcgacgtgt tctcgaatcc acacccgcgg
    14221 ctcgagcgcg gcggcgttgc tcgcgtcggg cggatggctc gtgatgcccg gcagcaccct
    14281 aacgcgcacg atctccgagc ccgccgcagt cgtccgacga acgtggcagc ccgtttccat
    14341 gcggaagaag tcgaaaacgc ccggccccac gagccgcagg accgcgagct tgactcggtt
    14401 ctggagcgcc tcatcgagcc cgcccacggg caccgtctgg atgtccccat cccaagtcat
    14461 caccgcgacg ccatccaaca ctccgcgcgc cttggcgtaa gagctcgcga gccagctgcc
    14521 gagccctgaa cccgccttcg agaaacgccg acgttcatgg tgtaacgaga gctgcaacac
    14581 ctcgatcaag accacgtgac ggccgccttc gtgcgccgtg tcgagtagtt tttcgagcag
    14641 cgcgacctcc acgagttctt gcaggagggc ttggctccgt cccaagctcg aagcttgccc
    14701 gagatgggtc ggcccactcg gagtgaactc cccgctgcgc ggacgtcgtg gatcacgcgc
    14761 aaagcgacgg ccggcatcgg ccccgaggat gctgaagtcc tgctcggtca gcgcctcacc
    14821 ccgggccacc ttctggcgtc ggtcagccgc ttgcaactcc gagtcgccac tgatgcgcgc
    14881 ttcgaggcgc tgacgcagcc gctcggcatg cgacagcaaa gcatccaaat tgaagacttg
    14941 gtccgcgcgg ctacgatcac ccaaacgaaa ttgactgagc tcgcggctca tacgctggcc
    15001 gagttcgggt aggcgcccgt ctcgctcgaa ggcggccaaa cgggtcaatg ccgcggggac
    15061 gtaggagcaa agctcagtgt agtcgcactc cagcaaatgc tgaacggggc tgctgcgtgc
    15121 gtggggggcg gcttcgcgca aagcctcgac gcgcacgcca acgccctgcc cgctctctcg
    15181 cgcgtgcatc cacaacaatc gcatttcggc ggggtggtct tgggtgagcg cctccgtgag
    15241 caccccaccc acctcacgct cgatgtgacg tttcagcgca cgtgctccga actccgggtc
    15301 gtaaccttct cgagtgaccc gctcggtcac cgcttccgtg aagcgcacga acacgttgcg
    15361 ttcgccaagg cccggacgcg ctaccaacga gctcaactcc ttgcggacaa tctcgcgcgc
    15421 ggcctgctcg tcgagcgtcg agaacggcac gattcggtcg atccggttga agagctcggg
    15481 cgggaagaac tcacgcaccg cgtgggctac gtgggccgca gccgcgttcg cgtcctcggc
    15541 gaagccacgc acggtggaac gcccggtgcc caagttgctg gtcatcacga tcagcgtgtg
    15601 ccgaaaatcg gtcacgttgc cgcgcgcgtc ccccaaccgc gcgtcctcga aaatctgcag
    15661 catcaggttc aaaacggtgg ggtgggcctt ctcgatctcg tcgaacagca agaccgcgaa
    15721 cggctgcgaa cgcaccgacg aggtgagcag gccatcgggc tcgatcgcat caccgattag
    15781 gcgcgccacc gcgtccggcc cgttgaactc gcccatgtcg aagcgcagca gccgctcctg
    15841 cgcgccgaat agatactccg cgaggcactt cgccagctcc gttttgcccg tgccggtggg
    15901 ccccgtaaac aacacgaccg agtaagggcg accggggccg acgagctggg ctcgcaagct
    15961 cagaatcaag tcgcatgcgg cctcgatggc ctgcgcttgt cccagcacgt gacgcgccaa
    16021 ctgccgcgcg agttcgtccc gggtgagcgg ggcgtcggcg gtgagcaaga gcttcggcaa
    16081 accggtctgg gtgctgaaga actcgagcac ctctgggctg ccgatggact ccaccgcgac
    16141 ggactgcgag ggatccattg attgttccgt cttgcccgct tgcgcgtgaa gccgggcaat
    16201 cgctttcaat ggggccagtg cgcgaccggg cagggccgta cctccgtaca aggtgccact
    16261 ctgttcgagg atggcgcgca gcgcacgcgg ctcgaaggca atgtccagct cctgctccaa
    16321 gcgccgactc tcgaacagca acatcgcgag cgactcttcc ggggtagcct cgctcaacag
    16381 cagggtctcg aacgcgctcg caaagccggg cgcctcgtac tccagggcgc ggtgctgttc
    16441 cgggctgcat tcgccgagca tcactagatc cccacgcgcc accgggccgc gaaagaaatc
    16501 ggccaatacg cgctcgctca ccgtcgttcg gcccaagctc gcgaaggccg gcaaatcgtc
    16561 gatccacaaa accagcttgc ggcgcttgca gcgcgcaagc agctccacgc agcgcgcctc
    16621 ccactgaccc acgtaagaca tgccggcgat gatctgtttg ccgctcaacg agagcacgtt
    16681 gtgcacgcga tccaaattgc catgggtcgg gtactcgtcg gcgtcgagca ggtcttgaac
    16741 ggcctgcgcc agcagcgccg acttccctgc gcccggcggc ccgaccacga gcacggagac
    16801 tttctttggc ccgcacaaca ggcgctgcag ttctcgccgt tcacgcggac gcggcaagcc
    16861 caagcgcaac aagccgctgg ccgcgcgcga cgtctggttg accgtgaccc gcatcagagc
    16921 ctcgagctgc ccagagacgc cgctagcggc aagcccttct tcgcgcttgg tgtctttcaa
    16981 cagtgtgcgc aggctcggag cgtccacgtg gaagcgcaac gtgaagagct tctcgttgcc
    17041 cgaacacttc agcgtttcaa gctcatcatc ggcttcgaag atgcgagaat aacggcgccc
    17101 gacgtccgtg acgagttctt caccttcgct tagttcgaac cagtcgctcg atcgcagcgg
    17161 atggtacgcg atgcgcatgc ggccagcggt tcccgcgtcg cgctcctcga tcaccaccgg
    17221 gaagtgaccg gtcaggcgtc gcttctcgcc ttggtggcgc agcacgagct gcaagtgcag
    17281 gcggttcaag tatctcccgg ccggcacgat gcagcggcgg aacgccgcgc gttcgcattt
    17341 agtggcctcc cggcgcacgc gctcgatcaa gcgctgtcgc gctttcccga ggtcacccga
    17401 ctgcaccgtc caattgaaca acccggggac gacgggcgtc agcgcgtaag agttcccctg
    17461 cttctgctgg taaacactaa tggcaatctg catgacgatt cagggctcga ggctcgtttt
    17521 caatcgcttc accggagagt tggtcaggcc agctcgtccg ggctggctcg atcgaaacgc
    17581 gacgggggca gaaagggctt accggactga acccgctcga gcacgcgctc gaacaggaag
    17641 tccgtcatcg aatcgaacag ctcagaaggc gagacggtca cccccgcggc gctagaattt
    17701 gccgcaagag cagggctcgc tgccgatcga gaagcgctcg cttccgcaaa ctcggcgagc
    17761 agcggctgcc cgcggcccac gctccgatcg atggacgcac gaaagtccga gtgctcccaa
    17821 tcctcggcgc gcacctcgaa gcgcggtctg agatagcgca cccagagttc tcccacgccc
    17881 tcgcgatcag ggcggcgata acgacccaga taaaacgcgg cgaacgcggc cgccccggcg
    17941 ccgcgcattc gcagcaacac ggcatcggcc gggcgcggct ccggcacctt cgtaagataa
    18001 gtattcatcg cgatgggcgg cccccagcgc cgctgctcga gcgtcggcca atcgccgtcc
    18061 cgagccacgc ggtcgaagtg cagcaccacc tcccagttct ggcgctgtgc gaagtgcagc
    18121 caaggcaata gcaacaagtg caacagacgt cggtcatcga gctcgtgaat cgcgagcgtc
    18181 acggtgttct gctgtgagga ctgcaacaga acccacaaca gctgcccgcg gaaacgctgg
    18241 tacgccaagc tcgcttccgg ttccaagtcg ggcggcgtct cgtcgatggc agacaacaac
    18301 aagccctcga tctcctcgat ctcacgcagc gcctcgtcca aaggatccaa ccagccccgg
    18361 acattcgcgt gctcgcgcga gagctccgac atgcgctgag cctgcagcgt cgaccgctca
    18421 gccgctcgcc ccgaggtccg cgcgacctcg gcgagcaaca cggtcaactc ggcgccgcgc
    18481 tcgcgcacct cgctcaaggg ctgcagcgcg ttccaacgcc gcatcgagcg ccgcatgcgg
    18541 ctcaccgagt tgatgcccga ctcgctctga gctcgctcac gctgccgggc caaagctgcg
    18601 gaaatcacga tcacctcgga caccgcaggc gccaagtccg aagggtagtg atcctcgagg
    18661 ctcgcgctcc acccgtcagg ccgggtcgcc ctgccctcgc tcgcgttcgc atcgtgctcc
    18721 gcgcgggagc acacgagcaa cttcgccccc tgggcctgcg ctccgagcgc cgagatcact
    18781 ccgctcaacg gcgcgaccaa cacttgctcc agataacgtc gcaggccacg cgctccgtac
    18841 tcgggagaga agcccccctg agcgagctgc aacagcgccg cctcggagat caagagctgg
    18901 gcgccgcgcg cttcgagccc cgcgcggcgc ccgatgcgat ccacgaacag ccgcgcaacc
    18961 tgaatgattt gttcggaatc caagctcgtg aaaccgatga tgccatccaa acgccccacc
    19021 agttcgggcc tgaaatgacg ttccacctgg cgctgatagt agctcgccga ctcttcctga
    19081 ccgccgctgc cgaagcccac ccgagcacgc cgctctctgg cacccaggtt gctcgtcagg
    19141 atcaagatcg tgttgtggaa ataggcaacc ttaccgcgcg catcgctgag tcggccttcg
    19201 cccgccactt gcaagagcag gtcgaaaacg ttggggtggg ccttctcgat ttcatcgagc
    19261 agcaataccc caaagggttg ctcgcgaacc cgctgtgtca gtgcgccgtc tgcgcgctcc
    19321 gaaccgcgaa tcagccgatc cgcggcgtac gcgtccatgt actcgctcat atcgaagcgg
    19381 gccatgcgct ctggggagcc gaataggaac cgagccagcg ccttggccag ctcggtcttg
    19441 ccaacgccgg ttgggcctag aaacaacaac gtcgcgagcg gcttcccggc tggttgtaga
    19501 cgagccttga tggtgcacaa gagctcggcc aagcgctgta cggcgtgatc ttgaccgatc
    19561 acgaactgct gcagaaaccg gcgcacgtcc gcgacgtgca ggttctgatc gtcgcgcagc
    19621 aagaactccg ggatgcccgt tttcgaggca aaggccgcca acacctgcgc cgggctcagc
    19681 tgagtcacgg ccccgctcgg gtcggaggat gccgcgtccc tggctcgggc gcgcagctcc
    19741 tcgtagagcc gcaccgcctt accgggcaag acccggtaag gttcgtagcg gtccgccaaa
    19801 tccaggatct gcgagagcgc cgccgaactc agcgcaagct cgcctcggcg ttgatcggcc
    19861 cgcacacgct cctgcagaat ggtcagagtt tgctcgcgcg tgagcggcgc cacattgaag
    19921 cgatgcaggt agctgaatat gccggcgtag cgctgcccca accgctcgta ggcttctgcg
    19981 ctcagctctg cgacgatttg aactctgccc tgctgcaaat gccgacgcag cagcgcggtc
    20041 atgtcttcgt gccccccttc gcggccagcg aagagatcct cgaagctatc gaagtagagc
    20101 gttgcttcca gcagctccgc cgcgctcatc acggcgtcga accgctcctg cagctggccc
    20161 aggcccgctt gccccgccac gatctccgct cccgaggtgg catagacgac acgcccgacc
    20221 tggctgaccg ccgcacggaa ccaatccgat tttccaaccc cctcgggccc aaccaacgcc
    20281 agactgcgcc gacccttccc agacactaaa ccttgtagct cctccatcgg gcgagaagcc
    20341 accgagtacg ccggcttgga aacgaggcgc cccacccgtt cgagcaaccg ctcggccgcg
    20401 cgacgctgcc gcaaagttgc cgaggatttc ccggccttgc gctccagccg ttgctccgga
    20461 tcgatctcta ccaaaggccg agatagctcg atctgcgcgg gaggaaacaa atccaggtac
    20521 gaggcgccgt cgatgtccgc ggcagccgct agacgcagga cctcctcgcg tacggcgtcc
    20581 tccacgtctt gggcgctact tgctgccacg tagctcactt gcccgacact cgggatcaac
    20641 aaccacttag caccggctgg tgccgccact tccacgatgg gcacctggag cgcggttcga
    20701 atgcgttcgg cgcgcggcag gtccgcgcgc ggaatgatga cactgacgtg gcgcaaggcc
    20761 gccgtctccg aaaccaagaa cgactgcagc tgatccggct ctagccgctc gaaatgatcg
    20821 cccaagaagt cgatcagctc ggattccgcc tcttcggagc tcgccccata ggcgatgaaa
    20881 tgcggagcac tggccgggaa agctgcacaa agcccctccg cgtaagcgca cgaaacaacc
    20941 aacggcctaa gcaccaacga agccacgccc acgcaactta tcacagcgtc gagcggcgcg
    21001 cgccgccgat ccgggcctgg atcgggaggg ggccgcggcg tagcccgcac gcttcggacg
    21061 cgctttgctg gaggcgcgct tcgcttcgaa cgctcacgag cctgaccctc acgcactggc
    21121 tggcagggta cccggagccg caccagaccg ccgggttggc aatcatactt agtattcacc
    21181 caccccagga agcgaacgag gagaacactg cggtacggca acgccgtatg catccaagtt
    21241 gcaaaaccga acgaatggaa ccgcctcgcc ccgtagatcc ccccgtagat ccggacgggc
    21301 aggcgcggtg cggtgaggga tggcaggcac ggcggctgcg ctggtgagga gtttggacat
    21361 gcagcgcgcg cgtagacctc ctgtggccaa atcccaattt tcagaagcga aatacgaaat
    21421 cgcttcgaga ggcatacgcg cgctctcgtt tcgcaaacgt tgaacccagt aattgctgca
    21481 gtcacaccgc tgcacgggag aattcgacct gggcgcaaac ctgcgtcgca aacttccacg
    21541 tcgtggaaac ttgtctaaca acacagtgtt ctagagcgtc taaagatggt tgtggaaaga
    21601 cagaccatcc cgcactgcga ctctggacat agggattgtt tcagtgaaaa ctgtgctgcg
    21661 ctatgcggac tttcttttcg ttgatcgcgc ttccgatcac cttcgtcgtc gcttgcagtg
    21721 atagccccgc ctcgggtgtc agctctggaa cgggcggagg gctctcgccg caaggcgggt
    21781 ctggcaacgc ggggactgcg aatacggcga atggtggcac tccgggttcg gcgagtggcg
    21841 gcacgggcac gaccagcatg gcgaacggag gagcgctgaa tagcggtggc tctccaatgg
    21901 cgagcggtgg ggccgcgatc acgagtaacg gtggcgcgag taacggtggc gcgagcacga
    21961 gccgaggcgg cgcgagcgca ggcggccaaa cgagttccgc aggggcgacg aacagctcgg
    22021 gcggcagctc gggaggagtc agcccatcga gcggaggcgg tggggccggc ggtactagtg
    22081 gggctgcgag cggaggcaaa ggcggagagg cttccaccgg cggctcgggc ggaggcaatc
    22141 ccggtggatg cacccgcgaa ctgctgaaga gcgcggtcga cgcttacttc aaagcgctcg
    22201 ccgcccacga ttcatcgacg ttaccagtcg cagagggcgt caagttcacc gaaaacggca
    22261 agtcgatgaa gcttggcgaa
//
LOCUS       DASTKU010000059        11652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_233136, whole genome shotgun sequence.
ACCESSION   DASTKU010000059 DASTKU010000000
VERSION     DASTKU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233136"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFQ61_02000"
     CDS             complement(<1..258)
                     /locus_tag="VFQ61_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953243.1"
                     /translation="MRRPHLAQKFMVLSALSGALLSASLARAQCGDDSECAKGFSCQV
                     LAVSCVAPACPPGQDCPTEPECDASEVRDCVPAKCSSDSDCA"
     gene            complement(555..1445)
                     /locus_tag="VFQ61_02005"
     CDS             complement(555..1445)
                     /locus_tag="VFQ61_02005"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9953244.1"
                     /translation="MNLAAIDTNLVIALHALLKERNVTRAAKRLGLGQSATSHALARL
                     RRLFGDALLVKAGRELVLTDRAEALLGPCTNAVAELERLFTEPSPFEPRSARRKFRIA
                     STDNLEAYVLPRLTAILAKEAPGVELHFFQLTTDWISGLKQDEFDLKLGRSYPIPSGL
                     RSEELFRDRLVCVARRGHPMGRRISLRQYAALSHIVVAPGRPERSFMDEALAAAGLER
                     RVALVVPHFLPALFAVAASDYVVTVPSRLMGVSPLRLRSVPLPVRAAEYALTQVWSDR
                     HDADPGHRWLRQTIRRSLAA"
     gene            complement(1553..2674)
                     /locus_tag="VFQ61_02010"
     CDS             complement(1553..2674)
                     /locus_tag="VFQ61_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953245.1"
                     /translation="MRAFANGLLLASLGLSVLVSACGSDSNAPGCVGDGCSAGGKSGG
                     ASGHSGGAHSGTNGGEPESDGGSAGRQSGSEPYGGAAGEGGMAPLEAGAPGKPSVNCG
                     DRSVDADEACDDGNQESGDGCRSDCRKIERCGDGARDLGEECDDGNDLDDDDCLNSCV
                     RAVCGDGVAQRRGSRRELCDDAGDSKTCDADCTTALCGDDYLNAIAGEECEDDGETER
                     CDADCTFPKCNDGYWNREVEQCDEGGNTTTCDADCTVPRCGDGFLNPEVEPCDTDGDS
                     AECNADCTLAKCGDGYRNAAAGEACDVQGESSRCDSDCTSNVCGDGHRNGSTEECDDG
                     NVDPEDGCTPDCKIQPNYICSPPGKPCCKACAADTCCPD"
     gene            complement(2710..5124)
                     /locus_tag="VFQ61_02015"
     CDS             complement(2710..5124)
                     /locus_tag="VFQ61_02015"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9953246.1"
                     /translation="MIQLGSLRFITSCVTLSLWASLLTSCYDTLTDDCGGWSCPDGWR
                     CTANRDSCTQTDCGDRVVQLDQGEQCDDGNTIDNDGCNSICKSELCGNGVVDPKEECD
                     GGDRVAPGPQDTLDCDADCTWRTCGDGHWNSALDPSGEAIEECDNGADANSDALYGVD
                     KGCTANCRLAPYCGDRVLDADREEECDPVGGAETRECNFGELARALGVACRERRCGDG
                     YVNLAEQCDPGENESSDCNRSPSGTARTDCQRSICGDGYTNEVAGEECDDGDAKHRTG
                     KYVNAEPQAVYGKTDRCTESCKRAPFCGDGRLDADVLNEDGEPEECDDGDDNAEASSI
                     YGQQGRCTTECRLAPFCGDQRLDSDVHDRQGKPFETCDDGNTVDGDRCSSTCDDASCG
                     NGVAESEEACDDGNGNNEDDCLTLTHELTNGLHSGTAAECRWNVCGDGYVNRTLKSDG
                     TPVEECDGGHLERIGDSWLPRPLDTEQCDSDCTVPRCGDGHLNPFTSDKEVCDLGSTT
                     DSAQCTAKCKIPGCGDSRLSPGEECDPTAEGFTPLNCDADCTLTVCGDGFVNATPDSS
                     GEPLEECDGGSPANPKRQATETCTDKCKISKCGDRVVNVEATEQCDPGLATPACTPWC
                     TESNHHDGYVDPALGEQCDTRGESKGCDADSTFVECGDGTRNRAAGEACDQGAPGVHV
                     PAEGCLDNCKRIGEGYECPADGGPCHKCEDGIVSGGEVCDEGKNSSGGCQDCKTIAPG
                     YVCPKDSEGNPLAGECSPDCGDGKVVGDEVCDEGVNPTGGCEMKCKKVATGYACPLKG
                     GPCTRK"
     gene            complement(5184..6320)
                     /locus_tag="VFQ61_02020"
     CDS             complement(5184..6320)
                     /locus_tag="VFQ61_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953247.1"
                     /translation="MTSLDPRRARRRAASATAALISILSTGLSAAQTPETQSPSLPTP
                     EQTDAAAAAGRAMRPIESAKAGSAWSTGVSEENRRAAREQFQEGNALLSNSAFAQAAV
                     KYRQALQLWDHPAAHYNLALALINLDQPIEVREHLLASLKHGPAPLDDEKFRHASSFL
                     KLLEQQLAPVNIECNAPGAIVTLNEKILFTAPGAYHGYVRAGEYVIRATKPGFEPTEM
                     RRPLLGGGETKIQLRLYTSEQLTETHRRWPTWVPFAVTGGGVLAAGVGTALLLSANSK
                     FRELDHLTAERCSEATTTGPGATPAGCSTTGEMNDLRESGQSHRTAGVITTAAGGAII
                     GAGAVLFVMNMAVSKRISPEEYEKLRVHPALSRDFAGLTASARF"
     gene            complement(6317..9160)
                     /locus_tag="VFQ61_02025"
     CDS             complement(6317..9160)
                     /locus_tag="VFQ61_02025"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9953248.1"
                     /translation="MAPAALATGTLTAIRPPPAPSLEVTSAPRAVPEQPEAAGAHAGA
                     SSEEASFLIGQYEVIRELGRGGMGVVYLARDVRLGRKVAIKLLQSRRPDFTARFITEA
                     RATARCSHENIVVIHEVGEFRGSPFMVLEFLEGQTLSTFLKRFGPLPASRAAELMASV
                     VRALVCAHSHGIVHRDLKPENIFITDSGTVKVLDFGIAKVLGVQPSAAALPIYQSMTS
                     TDRSGIAGTLAYMSPEQWGVSEIDHRTDIWAAGIVLFEMLTGVHPLVLVGKNPQHWVP
                     QLDQAMPSVLEHAPNTPSEFATLVGQCLRKLKVERVPDAVSLLKALEPFLPGRASLGP
                     IPIESGPYTGLRSFQEEDAGRFFGRDREIAAMAARVREFPLLAVVGPSGVGKSSFVRA
                     GIVPALKHSGENWSVAVTRPGREPLLALAALISPLIASSPTLVDDLGVQREIAQRLLH
                     EPGYFGSALRASARRNGQKLLLFVDQFEELYTLGAELRDRLAFTACLAGAADDVMSPV
                     RVVASIRSDFLDRVAEDPHFVNELSKGLFFLGPPSNQGLREALVRPAELAGYRFESPA
                     MVDEMVQHLEAVPGALPLLQFTAAQLWERRDVVRRQLTEESYQRIGGIAGALVSHADG
                     VVEKLGAELQVLCRTLFVNLVTIERTRAVRELRELREASGDPSELQRLLDHLVASRLL
                     IVQALDHGTTVEIVHESLITSWPKLRRWLDESHEDTIFLNQLLSAARQWQVRHDSGLL
                     WSGEMVEELARFQRRHPAPLPDFGAAFVKAVFDQYDRRRKRNRVLSVIAATLLAGLLA
                     AAVVVLVIVRNAQTAAERNAATARKAELLAKQSAAAAKTAELGARSSAETARIAEAAA
                     KQRLIDLERANRAREQSDQQRIEALTRENLTREELAKKNVMLQDALEHAERERLQARA
                     AEEKARLAEAREAKRANDLERILGSSLVPELKE"
     gene            9750..10058
                     /locus_tag="VFQ61_02030"
     CDS             9750..10058
                     /locus_tag="VFQ61_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HET9953249.1"
                     /translation="MIVEYIRYTVPTDKAAELVAAYQDGSASLRASPHCLEFELSQCR
                     DDAGSFVLRIVWDSLSGHLEGFRRSPEFQPFLQAIGRFVPNITEMRHYELTSVNWSRA
                     "
     gene            complement(10128..>11652)
                     /locus_tag="VFQ61_02035"
     CDS             complement(10128..>11652)
                     /locus_tag="VFQ61_02035"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9953250.1"
                     /translation="LLGGLASCTEASSPGTGGSSPTETGDGRGASAGHTLATGGSSGG
                     SPPMGTAATEPGGRSSNEGGGDAAGGRSALGGRSSSPGGGRTQAGGAGSAGALITGGT
                     WPGSRGTGGSSGVSSGGVAGSSSADASAEIYDPTQLPRFDIELSDASIKALNAVVSDA
                     DPKQNEYVHASLRYRNELMADVGLRIKGEGSFRRLDKKTAFKLKIDEYVDQQSFHGLR
                     RLTLNNMVEDPSFLAERLAYDLYRAAGLPAPRCNSALVYVNGDFYGVYANVEAEDKTF
                     LSRWFASNAGNLYEEGQADFVTGAELAFDLETNETANDRRGLEALIAAVQSAPQATFL
                     ADIDGSLNTQHFLRFTAAEAAVNQWDMYSYTMFYPNNFRLYENPSDRRFVFLPWGMDM
                     SMKPFRDSGRPHLSVFGIARQGDSKNGSISAGILFRKCLDSPACKAAYAEEVRQIAEV
                     YEQADLGSVAGRYYAQIKASVQADPRKEYTNAQFEQGYQVLLKTISERPAALRADID"
BASE COUNT         2063 a         3880 c         3472 g         2237 t
ORIGIN      
        1 cgcacaatcc gaatccgagc tgcactttgc cggcacgcag tcccgcacct cactcgcatc
       61 acactcaggt tcggtcggac aatcctgacc aggagggcac gctggggcca cgcaactgac
      121 ggccagcact tggcaagaaa accctttggc gcattcggaa tcgtccccgc actgggcgcg
      181 cgcaaggctc gcgctgagca acgccccgga gagggcagac aagaccatga acttctgtgc
      241 aagatgagga cggcgcattg cgtgctcctg aagcagccac catgccagat gaataatctc
      301 tgattttgct ggtcaatttt ctggatgcgc cgttcatcgc acgcacaccc cgtcgggttg
      361 tgccaccggt tgtgccagtt caggcgcaac gcgctcgttc gcgttttgcg gaaatttgca
      421 caccttctag ccgaggagcc gcgcgagacg acagggacgc gggctggcct cggagtgcgt
      481 ccttccttga ctgcaggttc ctacggaggg ttggaagtta tgaacggtct tgaatgggca
      541 cgtggttcgg gccgtcacgc cgccaaagag cggcggatgg tctgacgcaa ccagcggtgc
      601 ccaggatcgg cgtcatgtcg atcggaccag acctgagtca gcgcgtactc tgccgcgcgg
      661 accggcaatg gcacgctacg caaccgcaag ggagagactc ccatcagccg tgaaggcacg
      721 gtgaccacgt agtcagaggc cgcgacggca aacagggccg gaaggaaatg aggcaccacg
      781 agggcaacgc ggcgctcgag ccccgcggcc gcgagtgctt cgtccatgaa gctccgttcg
      841 ggacggccgg gcgccaccac gatatgcgac agcgcggcgt actgacgtag cgaaatccgg
      901 cgccccatcg gatgcccgcg tcgcgccaca caaacgagcc gatcgcgaaa cagctcctca
      961 ctgcgtaacc ccgaagggat tgggtagctt cgaccaagct tcaagtcgaa ttcgtcctgc
     1021 ttcaacccag agatccaatc ggtggtcagc tgaaagaaat gaagctcgac ccccggagct
     1081 tccttggcga ggatggcggt caagcgcggc agaacatagg cctccaagtt gtccgtcgat
     1141 gcgatccgaa acttgcgacg agcactccga ggctcgaacg gcgatggctc cgtgaacaaa
     1201 cgctcgagct cggcgaccgc attggtgcat ggccccagca gagcctccgc gcgatcggtg
     1261 agcaccagct cgcgccccgc tttgaccaga agcgcatcgc cgaaaagccg gcgcaagcgg
     1321 gccagagcgt ggctcgtggc cgattgaccc aagccgaggc gcttggccgc gcgcgtgaca
     1381 ttgcgctcct tcagcagcgc gtgcagagca atcaccaggt tggtgtcgat cgcggcgaga
     1441 ttcataggcc gcgcaggatg tgccgccggg cgccctcgag caaatccatc gagcggcgga
     1501 ggtatgaatc gtgtcgatat ctcggcctat cggccctgca ccaaggacgg cactagtctg
     1561 ggcagcacgt atccgcggcg caagctttgc agcagggttt gccgggcggg gagcagatgt
     1621 aattgggttg aatcttgcag tccggcgtgc acccatcctc cggatccacg ttgccatcgt
     1681 cgcactcctc agtggagccg ttgcgatggc cgtcgccgca gacgttcgag gtacagtcgc
     1741 tgtcgcaccg cgacgactcc ccttgcacgt cgcaggcttc accggccgcc gcgtttctat
     1801 agccatctcc acacttggcg agggtgcagt ccgcgttgca ctccgcgcta tcaccatcgg
     1861 tatcacacgg ttctacctca gggttcagga aaccatcacc gcagcgtggg accgtgcaat
     1921 ccgcgtcaca ggtggtggta ttgccgcctt cgtcgcattg ttcgacctcg cggttccaat
     1981 aaccgtcatt gcatttggga aaggtgcagt ccgcgtcgca acgctcggtt tcgccgtcat
     2041 cctcgcattc ttcaccggca atcgcgttga ggtagtcatc gccacacagc gcggtggtgc
     2101 agtctgcatc gcaggtcttc gagtcccccg catcatcaca taactcgcgc cgagacccgc
     2161 gacgctgcgc cacgccgtcg ccacaaacgg cgcgaacgca gctgttcaaa caatcatcgt
     2221 catccaggtc attgccgtcg tcgcactctt ctcccaaatc tcgagctccg tccccgcaac
     2281 gctctatttt gcggcaatcc gagcgacaac cgtcgccgct ctcctgattg ccatcgtcac
     2341 aagcctcgtc cgcatcgacc gagcgatcgc cgcaattcac gctgggcttc ccgggagctc
     2401 ccgcctcgag cggcgccatc cccccctcgc cggcagcccc tccgtagggt tcggagcctg
     2461 attgccttcc ggcactgccc ccatcggact cgggctcgcc accgttggtt ccagagtgcg
     2521 ctccgcccga gtgcccgcta gctccgccgc tctttccgcc cgcggagcac ccatctccta
     2581 cacatcccgg cgcattggaa tcggagccac aggccgacac caaaaccgat agacccagcg
     2641 acgctaggag aaggccgttc gcgaacgctc tcatgtttcc tcgcccagtc acgctcaccg
     2701 cactttacgt cacttccttg tacacggtcc gcctttcagc gggcacgcat accccgttgc
     2761 gaccttcttg cacttcatct cgcaacctcc agttggattc acgccctcgt cgcagacctc
     2821 gtcgcccacc accttgccgt ccccacaatc gggtgagcac tcccccgcta acgggttccc
     2881 ctcgctgtcc ttagggcaca catacccggg cgcgatcgtc ttgcagtcct ggcagcctcc
     2941 gcttgaattc ttgccctcgt cgcaaacttc gccgccgctc acgatcccgt cttcgcactt
     3001 gtggcagggg cctccatccg ccggacattc gtagccttca ccgatccttt tgcagttatc
     3061 gagacaaccc tcagctggca cgtgcacccc cggcgcacct tgatcgcaag cctctccagc
     3121 ggctctgttg cgcgtcccat ctccgcattc cacgaacgtg gagtctgcat cgcaaccctt
     3181 ggattcgccc ctcgtgtcac attgctcacc cagagcgggg tcgacgtaac cgtcgtggtg
     3241 attcgattca gtgcaccaag gcgtgcatgc gggggtcgct aaacccggat cacattgttc
     3301 tgtcgcctcc acattgacca ccctgtcccc gcacttcgag atcttgcact tgtccgtgca
     3361 agtctcggtg gcctggcgtt tcgggttcgc gggtgatccg ccgtcgcact cttcaagggg
     3421 ttcaccgctc gagtcgggag tagcgttgac gaaaccatca ccgcacaccg tgagagtgca
     3481 atcggcgtcg cagtttagcg gggtaaagcc ttcggcggtg ggatcacact cttcgccggg
     3541 ggaaagtcga ctgtcgccgc agcccgggat tttgcatttt gccgtacatt gcgccgagtc
     3601 cgtcgtactc cccaggtcac agacctcttt atccgaagtg aaggggttta gatgtccgtc
     3661 accgcaacga ggcacggtgc aatcgctatc acattgttcc gtatccaggg gtcggggcag
     3721 ccagctatcc ccaatccgct ctagatgccc cccgtcacac tcctccacag gtgtgccgtc
     3781 gctcttcagg gtgcggttca cgtagccgtc tccgcagacg ttccagcggc actccgcggc
     3841 ggtcccgctg tgcagaccgt tcgtgagctc gtgagtcagc gtcagacagt cgtcttcgtt
     3901 gttcccattc ccgtcatcgc aggcctcttc ggattcggcg acgccatttc cacagctcgc
     3961 gtcgtcgcag gtggagctgc accggtctcc atctacggtg ttgccgtcgt cacaagtttc
     4021 gaagggcttt ccttgtcggt cgtgcacgtc gctgtcgagc cgttggtccc cgcaaaaagg
     4081 agccaatctg cactcggtag tgcatcggcc ttgctgtccg tagatgctgg aggcttcggc
     4141 gttatcgtca ccatcgtcgc actcctcggg ttcgccatct tcatttagaa cgtcggcatc
     4201 gagtctccca tcgccgcaaa acggtgctct cttgcaagac tcggtacacc gatccgtctt
     4261 accgtagacg gcctggggct ccgcgttcac atacttgccg gtgcggtgct tcgcgtcacc
     4321 gtcgtcgcac tcctcgcccg cgacctcgtt cgtgtatccg tccccacaga tcgagcgctg
     4381 acaatcagtt cgcgcggtgc cactcgggct gcgattgcaa tctgaactct cgttctctcc
     4441 aggatcgcac tgctccgcga gattgacata accgtcaccg cacctcctct cgcggcaggc
     4501 cacgccgagc gctctcgcca attcgccgaa gttgcattct cgcgtctcgg cgccccctac
     4561 cggatcgcac tcctcctccc gatccgcgtc gagcactcga tcgccgcaat agggggccaa
     4621 acggcaattt gccgtacacc ccttgtccac accgtagagt gcgtcactgt tggcgtcggc
     4681 tccgttgtcg cattcttcga tcgcctcccc gctggggtcg agcgctgaat tccagtgccc
     4741 gtcgccacac gtgcgccagg tacagtccgc gtcgcagtcg agggtatcct gcggtcccgg
     4801 cgccacccgg tcgccgccat cacactcctc cttcggatcg acgacgccgt tgccgcagag
     4861 ctcgcttttg cagatagagt tgcagccgtc gttgtcgatg gtgttgccgt cgtcacactg
     4921 ctcgccttgg tccagctgca ccacccggtc accgcagtcg gtctgggtgc agctgtcccg
     4981 attggccgtg cagcgccagc catccggaca gctccaaccg ccgcagtcgt cagtcagcgt
     5041 gtcgtagcag ctcgtgagca agctggccca aagcgacaga gtcacacaag aggtgatgaa
     5101 gcgaagcgaa cccaactgaa tcatagatac tccccgcgtg ttgaagcgcg gcaccttctc
     5161 tgaatggaga atttgcgaac ggcttagaag cgagcggacg cggtcaatcc cgcgaagtct
     5221 cgactgagcg ccgggtgaac gcgtagcttc tcgtattctt cggggctgat gcgcttcgag
     5281 accgccatat tcatcacgaa tagaaccgca ccggccccga tgatggctcc gcccgccgcg
     5341 gtggtgatca cgcccgcagt ccgatggctc tgtccggact ccctcagatc gttcatctca
     5401 ccggtggtgc tgcagccggc cggcgtagcg ccgggtcccg tggtggtcgc ttccgagcag
     5461 cgctccgccg ttaggtggtc gagctcgcgg aacttggagt tggcgctgag taggagcgcg
     5521 gtgcccacgc ccgcagccag gactccaccc ccggtcaccg caaacgggac ccacgtgggc
     5581 caccgacgat gagtctcagt cagctgctcg gaggtgtaga ggcggagttg gatcttggtc
     5641 tcccctcccc cgagcagcgg gcgccgcatc tccgtaggct cgaaccccgg tttggtggcg
     5701 cgaatcacgt actctcccgc gcgcacgtag ccatgatatg cgcccggagc cgtgaacaaa
     5761 atcttctcgt tcaacgtgac gattgcaccc ggcgcattgc actcgatgtt cacgggggcc
     5821 agctgctgct cgagcagctt gaggaagctg ctcgcgtgcc tgaatttctc gtcatccaaa
     5881 ggtgcgggcc cgtgtttgag ggaggcgagc aggtgctcgc gcacttcaat cggctggtcg
     5941 agattgatca gcgcaagggc caggttgtag tgcgcagcgg ggtgatccca gagctgcaaa
     6001 gcctggcgat acttgaccgc agcctgtgcg aacgcagagt tgctcagcag cgcgtttccc
     6061 tcctgaaatt gctcccgcgc cgctcttcta ttttcctctg acacaccggt gctccaggcg
     6121 gagcccgctt tcgcggattc gatggggcgc atggcgcgac ctgccgcggc agcggcgtcg
     6181 gtctgctcgg gcgtgggcag tgagggtgac tgcgtctcgg gagtctgagc cgcggacaga
     6241 ccggtgctca ggatcgaaat caacgccgcg gtcgcgctgg cggcccgccg acgcgcacgc
     6301 ctcgggtcga gtgacgtcat tccttgagct ccggcacgag cgaactcccc aggatcctct
     6361 ccaagtcatt ggcgcgcttg gcctctcgag cctcggccag gcgcgccttt tcctccgcgg
     6421 cgcgcgcctg caagcgctcc cgctcggcgt gttcgagcgc gtcttgcagc atgacgttct
     6481 tcttcgcgag ctcttcccga gtcaggtttt cccgggtcag cgcctcaatc cgctgttggt
     6541 cggattgttc ccgagctcga ttcgcgcgct cgagatcgat caagcgctgc ttggcggcag
     6601 cctccgcgat gcgcgcggtt tccgcgctgc tgcgcgcgcc cagctcggcc gtcttggcgg
     6661 cggcggcgct ctgcttggcg agcaactctg ctttgcgcgc ggtggcggcg ttgcgctccg
     6721 ccgccgtctg agcattacgc actatcacca acaccaccac ggccgccgcg agcaagcccg
     6781 cgagcaacgt cgcggcgatc acggacagca cgcgattgcg cttccgtcgg cggtcatact
     6841 gatcgaatac ggccttcacg aacgccgccc cgaaatcggg caggggcgcc gggtgacgac
     6901 gctggaagcg agcgagctct tccaccatct cgccactcca gagcaagcct gaatcatgcc
     6961 ggacctgcca ttgtcgagcc gccgacagca gctgattcaa gaagatggtg tcttcgtgac
     7021 tctcgtcgag ccagcgccgc aacttgggcc agctggtgat gagggattcg tgcacgatct
     7081 cgaccgtcgt gccgtgatcc agagcctgaa cgatcagcag ccgtgaagcg accaggtgat
     7141 cgagcagccg ctggagctcg ctcggatccc cgctcgcttc acggagctcg cggagctcac
     7201 gcaccgcgcg agtccgctcg atggtcacca agttcacgaa caacgtccga cacagcacct
     7261 gaagttcagc gccgagcttc tcgaccacac cgtcggcgtg gctgaccaga gctccggcga
     7321 tgccaccgat gcgctggtag ctctcctcgg tgagctggcg ccgcacgaca tcacgccgtt
     7381 cccaaagctg tgcggccgta aactggagca gcgggagcgc ccccgggacg gcttccaagt
     7441 gctggaccat ctcatccacc atagctgggg actcgaagcg gtagccggcg agttcggcgg
     7501 gtcgcacgag cgcttctctc aagccttgat tgctaggggg ccccaggaag aataggcctt
     7561 tcgagagttc attcacgaag tgaggatctt cggcgacccg gtccaagaag tcagagcgaa
     7621 tggaggccac cacccgcacc ggcgacatga cgtcatcggc cgcgcccgcc aagcaggcgg
     7681 tgaaagccag ccgatcgcgg agctctgctc cgagtgtgta gagttcttcg aactgatcga
     7741 cgaacaggag cagcttttgc ccgttgcgcc gtgcgctcgc gcgcaacgcg ctgccgaaat
     7801 agccgggctc atggagcagc cgctgagcaa tctctcgctg cacccccagg tcgtccacca
     7861 gcgtcgggga actagctatc agcggagaga tcagcgccgc gagcgcgagc aggggctcgc
     7921 ggccggggcg cgtgacggcg acgctccagt tttcccccga gtgcttcaag gccgggacga
     7981 tccccgcgcg cacgaaggac gatttcccca ctccggaggg ccccaccaca gccagaagtg
     8041 ggaactcccg aacccgggcc gccatcgcgg cgatttcgcg gtctctgccg aagaaacgcc
     8101 cggcgtcttc ctcttggaaa gaacgcaggc ccgtgtaggg tccactctcg atcggaatcg
     8161 gccccagcga agcgcgtccg ggaaggaacg gctccaatgc tttgagcagg gacaccgcgt
     8221 ccgggactcg ctcgaccttg agcttgcgca aacactgccc gaccaaggtc gcaaactccg
     8281 agggcgtatt cggggcgtgc tcgagcacgc tcggcatggc ctggtcgagt tgcgggaccc
     8341 agtgttgtgg attcttacca accagcacca gggggtgaac cccagtgagc atttcgaaca
     8401 ggacgatgcc ggcagcccag atgtccgtac gatggtcgat ctcggagacg ccccactgct
     8461 cgggcgacat gtatgccagt gtgcccgcga ttccactgcg atccgtgctc gtcatcgatt
     8521 ggtatatcgg aagggcggcg gctgagggtt gcacccccaa gactttcgcg atcccgaagt
     8581 cgagaacctt caccgtgccc gaatcggtga tgaagatatt ctccggcttc aagtcacggt
     8641 gcacgatgcc gtgcgaatgg gcgcagacca gggcgcgcac gacggaggcc atgagctcgg
     8701 cggcacgcga cgcagggagc ggcccgaagc gtttcagaaa ggtggataac gtttgcccct
     8761 ccagaaactc cagcaccatg aagggactac cccgaaactc cccgacctcg tgaatgacga
     8821 cgatgttctc gtgactgcaa cgcgccgtgg cacgcgcctc ggtgatgaac cgagccgtga
     8881 agtcaggcct tcgcgactgc agtagcttga tggctacttt cctgcccagc cgaacgtctc
     8941 gggccaaata gacgacaccc ataccgccgc gacccagctc gcggatgact tcatattgac
     9001 caatcagaaa ggatgcttcc tcgcttgaag cgccggcgtg cgcgcccgcc gcttcgggct
     9061 gctccggcac ggcacgggga gcactggtca cttccaagct cggcgcgggc ggcggacgaa
     9121 tggcggtcaa ggtcccggtc gcaagcgcgg ccggtgccat cgaatcagtg aacccttcga
     9181 aggggagatc gccggatccg ctctgcctct tcggcacgac gtccgagcgc cgttcgcccg
     9241 aatcccgcga aaacggctcg tcttgagagt ctggtttaac cttcattgtc ttccccggcc
     9301 gcagtggtgt ccaactgctc atcaactcgc atcgattgcc gcgaagctcg agctgaagag
     9361 cttgactata cacggctcac aactggcgtt gaaggctcac ggatggtgat ttatacattt
     9421 cgagtccctg gcgatcttcg ccggagccgc agccacgata cccccagcgc gcgccggccc
     9481 aggaggaccc ttctggcgga ggggcggcga gagcgaggct actggttcgc gttacgcccg
     9541 gcagaccgag atgtagctcg cgcccagttt cgagctgcgc acggctgttg gcgcgggtgt
     9601 tggcgcggcg tgcctcccgc gtggagccac gcgtatgaac tccatcgata cctcgcctca
     9661 tcgatggctg gtatgcgggc aatccatggg caaggcgtcg cgactgcggt caagacttgg
     9721 ggaacccggc ctttgggcaa aggatgtcaa tgatcgtcga atacatacgt tacacggtgc
     9781 ctacggataa agcggcagag ctcgttgcgg cttaccaaga tggcagcgcc agcttgcgcg
     9841 cttctccaca ttgtcttgag ttcgagctca gccaatgccg agatgacgcg ggctccttcg
     9901 tgcttcgcat cgtttgggac tcgctctccg gacatctcga gggcttccgg cgatccccgg
     9961 aattccaacc ctttctccag gcgatcggtc gtttcgtgcc gaacatcacc gagatgcggc
    10021 actacgagtt gacgtccgtg aactggagcc gggcatagac gcttttctcg atgggcccag
    10081 cccgctgatt ggggtgggct ctcgggcgtg ctctccgggg ggggccgcta atcgatgtcg
    10141 gcgcgcaacg ccgcgggccg ctcgctgatg gtcttgagca gcacctgata accctgttcg
    10201 aactgcgcgt tcgtgtattc cttgcgcggg tcggcttgca ctgaggcttt gatttgcgcg
    10261 tagtagcggc ccgcgacgct acctagatcg gcttgctcgt aaacttcagc aatctgccta
    10321 acttcctcgg catatgcagc cttacaggcg gggctgtcca ggcacttgcg gaataggatc
    10381 ccggcgctaa tcgagccatt cttggagtca ccctggcgcg caatgccgaa caccgatagg
    10441 tgcggtcggc ccgagtctcg aaagggcttc atcgacatat ccatgcccca cggcaggaac
    10501 acgaatcttc gatcggaagg attttcgtat agcctgaagt tattgggata gaacatcgtg
    10561 tacgagtaca tgtcccactg attcacggcc gcctccgcgg cggtgaatcg gaggaagtgc
    10621 tgggtattga gggagccatc gatatcggcc aagaacgtcg cctgcggcgc cgactggacg
    10681 gcggcaatca gcgcctcgag tccgcggcga tcgttggcgg tctcgttggt ctcgagatcg
    10741 aaggcgagct cagcgccagt cacgaagtct gcttgccctt cctcgtacaa gttgcccgcg
    10801 ttgctggcga accaacgact gaggaacgtt ttgtcctcgg cttcgacgtt cgcgtacacc
    10861 ccgtagaagt cgccattcac gtacaccagc gcgctattgc agcgcggcgc cggtaaaccc
    10921 gccgcgcggt acaagtcgta ggccagccgc tccgcgagga acgacggatc ctcgaccatg
    10981 ttgttgagtg tgagccggcg cagcccgtga aaggactgct ggtcgacata ttcgtcgatc
    11041 ttgagcttga acgccgtctt cttgtcgagc ctgcggaagc tcccttcgcc tttgattcgc
    11101 aatccgacat ccgccatcag ctcgtttcgg taacggaggc tggcgtgcac gtactcgttc
    11161 tgttttgggt ccgcgtcgct cacgaccgca ttcagggcct tgatcgaagc atcggataat
    11221 tcgatatcga aacgcggcaa ttgcgtaggg tcgtagattt cggcgctggc gtccgccgaa
    11281 gaggagcctg caactccgcc gctgctcacg cccgaacttc cgccggtccc acgggaaccc
    11341 ggccaagtgc cccccgttat cagtgctcct gccgagcctg cgccgccagc ttgagttcta
    11401 ccgccacccg gagaactcga ccttccgcca agtgccgaac gcccccccgc tgcgtccccg
    11461 ccaccttcgt tcgaggagcg cccgcccggt tcagtagcgg ccgtacccat cgggggagac
    11521 ccacccgagg agccaccggt cgccagcgtg tgacccgcgg aagcccccct tccatctccc
    11581 gtctcagttg gagagctgcc gccagtgccg gggctgctgg cctcggtgca agaggcgagt
    11641 ccgcctaaca ac
//
LOCUS       DASTKU010000060         5367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_233519, whole genome shotgun sequence.
ACCESSION   DASTKU010000060 DASTKU010000000
VERSION     DASTKU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233519"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VFQ61_02040"
     CDS             complement(<1..598)
                     /locus_tag="VFQ61_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953251.1"
                     /translation="MIPSRWEFGSDFHSLPPGECCVRLRPAAPVKAALRSQPVAPETT
                     RASLPAEAHPHTFWASGRDALVALLQWAKPTLSLNRLWCPSYLCQPMLHAAKAADLSL
                     VAYPDSPLSSRAETSRLELRSGDMVLAVGYFGLRGPALRLPPRRSASIYVLEDHSHDP
                     WSNWAAASKADFCFASLRKTLPIPDGAVLWSPRGWVGPS"
     gene            complement(763..3096)
                     /locus_tag="VFQ61_02045"
     CDS             complement(763..3096)
                     /locus_tag="VFQ61_02045"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9953252.1"
                     /translation="MLLSTWSPRLVRSALGVMSLAAVPQLVACGSEQGPKRAPSSSGG
                     AANTGGAGGAVSAAGGTTTVPPLIERPGGTVEDPCKRPDAPPNCELTAPPGCGDGLIN
                     QPSEDCDDGNPLPGDGCSGLCKVEVNFACTTPGQPCRSTIVCGDGTLGPGEACDDGNL
                     DAGDGCAADCRAVEPGYVCRTQAMPCTRVFVCGDQRVDPNEGCDDGNGMDGDGCDARC
                     RIELGFKCDGSPSTCSPTKCGDKKVEGAESCDDGNALPFDGCSPDCRAEPSCANGPCS
                     SKCGDGIVLNEACDDGNLRDGDGCSSQCQTEPAFTCKNDDPCEMVDGKCTLRVPAIYR
                     DFKPANASGHPDFQPSANNETKIVGLVKNELDAESKPVFSGKTGGNIRSVESFAQWYR
                     DVPNVNKSLVGELVLWDNGSGGYVNRWGMNGERFVGYPGLLGVDQRPAGAPVNSRFVW
                     CANAMQGGCDAEACLPAANEACIFPCTPNNNNDACVGTVMYFDGNPLFFPVDSLGDRT
                     SAAKIPPAYAWNWSYEPPTANPPKHNFHFTSEVKYWFQYDAAGSAKLDFTGDDDVWVF
                     VNGILAVDLGGWHGPLDGSVTIDRASADKFRLADGKVFQISIFHAERMTEGSSFRLTL
                     SGFKNAPSDCRAQCGDGQIAAGEECDDGTAMNLGGYNQCTSSCRLGPRCGDGVVQPEF
                     QEICDEGTAKNTGAFGSCAPNCQPGPHCGDGAVDPDNEQCDDGMNLGSYGGCGPTCML
                     GPHCGDGKVQVEYEDCDDGNTVGNDGCSVACRIEIPR"
     gene            complement(3348..3740)
                     /gene="speD"
                     /locus_tag="VFQ61_02050"
     CDS             complement(3348..3740)
                     /gene="speD"
                     /locus_tag="VFQ61_02050"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HET9953253.1"
                     /translation="MRTTGQHYLIEFFGCASERLDDARQLGQLLRASAELAGARVVGE
                     LVHPFSPHGVTALVLVEESHLSIHTWPETGYAAVDFYTCGDVRAEKAIQLLAEQLRAR
                     EIVSLEVRRGGPPETTLGVGIRESRTRA"
     gene            complement(3762..4976)
                     /locus_tag="VFQ61_02055"
     CDS             complement(3762..4976)
                     /locus_tag="VFQ61_02055"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HET9953254.1"
                     /translation="MLAAEHVQVRRTGERLVLVELSAKKRLRALELAEQILETARAHV
                     QATRDDLDEALGLIGVAPNERKLFEGLKKLVEEYCEFGETPVVDPAALRSELFAKAAQ
                     RRKHSEVAFDRAALVQEVAAERGLDVQQLEDVLYADLRGAHRLLAVPQVAAAELVTQY
                     DRGQVQAILLRAVSLVVNVTCKSPDAYRALFHKLKFRRLLYRMERRDPSGYRLEIDGP
                     FSLFDAGTKYGLELACTLPALQACDLLELSARVLWGKQRTPLLFEYRSQAAQPDADAA
                     HVMRDEVRELCEDFAELSEFRCRPCEEIFEVAGQGVCVPDLVFEPTRGGAPIYLEMLG
                     YWSRDAVFRRVELVERGLAPNLLFAVSSKLRVSEEVLASEHSALYVFKGRPNARAILR
                     KLQEIHAKRGKR"
     gene            complement(4970..>5367)
                     /locus_tag="VFQ61_02060"
     CDS             complement(4970..>5367)
                     /locus_tag="VFQ61_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET9953255.1"
                     /translation="DLHRQERSLIFTQDNRMAYAVSKQFLIPVITHQTKVKERSEILE
                     RFARGEYTAIATSKVLNEGVDVPEASVAVVISGSGSVREHVQRLGRILRRKDGKRAVL
                     YELVSSGTGEAFTSERRREHAAYQAPPKC"
BASE COUNT         1004 a         1743 c         1631 g          989 t
ORIGIN      
        1 cgctcggacc cacccagcca cgcggtgacc agagtaccgc gccgtccggg atgggcagcg
       61 tcttgcgcaa agatgcaaag cagaaatcgg ctttgctcgc ggcggcccag ttggaccatg
      121 gatcgtgcga atgatcctcg agcacgtaaa tgctggcgga gcggcgaggc ggtaagcgca
      181 aagctgggcc gcgcaaaccg aagtacccaa ccgccagcac catgtctcca gagcgtagtt
      241 ccagtcgaga tgtctctgct cgggaagaga gcggcgagtc ggggtacgcg actagggaga
      301 gatcggccgc cttggccgcg tgcagcattg gctggcacaa gtagctcggg caccagagcc
      361 gattcagcga aagagtcggt ttagcccact ggaggagcgc cacgagcgcg tctcgcccgg
      421 aagcccagaa tgtatgtggg tgcgcttccg ccggcaggct ggctcgagtc gtttccgggg
      481 cgaccggctg tgaacgcaac gccgccttta ccggcgcggc cggacgcagg cgcacgcagc
      541 actctccggg gggaagtgag tgaaaatccg agccgaactc ccagcgagac gggatcatgc
      601 ctgagagcac gatctagtcg ctggtcatgc ccagctcaag ccgcttctga gccgcccgat
      661 cccgcgtccg gcccccttcg gaggatgaag cacggcgccg ggacgcctcg tagctttttg
      721 gcagcaggcc gtgctcgcga ccggtgatcg ggggagccac gatcagcggg gaatctcgat
      781 gcgacaggca acgctgcagc cgtcgtttcc aacggtattt ccgtcatcgc agtcctcgta
      841 ctccacctgg actttcccgt ctccgcaatg cggacccagc atacaggttg ggccgcagcc
      901 gccatagctt cccaggttca tgccgtcgtc gcactgctcg ttatccgggt cgaccgcgcc
      961 atcaccgcag tgcgggccag gttgacagtt cggagcgcag ctgccgaagg cgcccgtatt
     1021 cttggcggtg ccctcgtcgc agatttcttg aaactcaggt tggaccacac cgtcgccgca
     1081 gcgcggtccg agtcggcagc tcgacgtgca ttgattgtac ccccccaggt tcatggcggt
     1141 gccgtcgtca cactcttccc cagccgcgat ttgcccatcg ccgcattgcg cgcggcaatc
     1201 gcttggggcg ttcttgaaac cagagagcgt gagtcggaag gacgagcctt cggtcatgcg
     1261 ctcagcgtgg aagatggaga tttggaagac cttcccatct gcgagcctga acttgtccgc
     1321 ggatgcccgg tcgatcgtga cgctaccatc gagcggcccg tgccaaccgc cgaggtcgac
     1381 ggccaggatg ccattcacga acacccagac gtcgtcatca ccagtgaagt cgagcttggc
     1441 ggagccggcg gcgtcgtact ggaaccagta cttgacctca ctcgtgaagt gaaagttgtg
     1501 tttgggtgga ttggccgtgg gtggctcgta ggaccaattc caggcgtacg cgggcgggat
     1561 cttggcggcg ctggtgcgat cccccagtga gtccaccggg aagaacagcg gattcccgtc
     1621 gaaatacatg acggtaccca cacacgcgtc gttgttgttg ttgggtgtac agggaaagat
     1681 gcacgcttcg ttggcggccg gcagacaggc ttccgcgtca cacccgcctt gcatggcgtt
     1741 cgcgcaccaa acgaagcggc tgttcaccgg cgcgcccgcg ggacgttggt cgacgccgag
     1801 cagaccgggg taaccgacga atcgctcccc gttcatgccc cagcgattca cgtatccccc
     1861 gctaccgttg tcccacagaa cgagctcccc cacgagcgac ttgttcacgt tcggtacgtc
     1921 acggtaccac tgggcgaaac tctccacgct gcgaatgttg ccgccagttt taccgctaaa
     1981 gactggcttc gactccgcat cgagctcgtt cttcacgagc ccgacgatct tggtctcgtt
     2041 gttggcagaa ggctggaaat cagggtggcc actcgcgttc gcgggcttga aatcgcggta
     2101 gatggccggc acgcgcagag tgcattttcc gtctaccatc tcacacggat cgtcgttctt
     2161 gcaggtgaag gcgggttcgg tctgacactg actcgagcag ccgtctccgt cacgcaggtt
     2221 gccgtcgtcg caggcctcgt tcagaacgat gccgtcgcca cacttcgagg agcatggccc
     2281 gttcgcgcag ctcggctccg cccggcaatc cggagagcag ccgtcgaagg gtagtgcgtt
     2341 gccatcgtcg cagctttctg cgccctcgac cttcttatcg ccgcacttcg tgggtgagca
     2401 tgtactggga gaaccatcgc acttgaagcc gagctcgatc ctgcatcgtg cgtcgcaccc
     2461 atcgccgtcc atcccgttgc catcgtcgca accttcgttg ggatccaccc gctgatcgcc
     2521 gcacacgaac acgcgggtac agggcatcgc ttgggtgcgg caaacgtagc cgggttctac
     2581 ggctcggcag tccgctgcac agccgtcgcc tgcgtcgagg ttgccgtcgt cgcaggcttc
     2641 gcccgggccg agtgttccgt ctccacaaac gatcgtgctc cgacaaggct gcccaggagt
     2701 ggtgcaagcg aagttaacct ccactttgca gagccccgag cacccgtctc ccggcagcgg
     2761 gttcccatcg tcacagtctt cgctgggctg attgatgaga ccgtctccac aacccggtgg
     2821 tgcggtgagc tcgcagttcg ggggggcatc gggacgctta caagggtcct cgaccgtgcc
     2881 acctggcctc tcgatcagag gaggcaccgt cgttgtccct ccggccgcgc tcaccgcccc
     2941 ccccgcacct ccggtgttcg ccgcacctcc actcgatgat ggcgcccgct tcggcccctg
     3001 ttcagagccg caggcgacca gctgcggcac cgccgccaaa ctcatcaccc ccaaagcact
     3061 acggacgaga cggggactcc aagtcgaaag aagcatgacg ttggccctcg caagcaagtt
     3121 aagcagagga acggcttccg ctaagaatag ctagtgcgcg cctcataggc cacgacaacg
     3181 cacgtgaacg aatgatttcc caacgactcg cagagcgcgc acgagcaatc tgctcgggac
     3241 cgaatatccg cgcgtcccag caagcggccg ccggggacgg aatcttcgcc gtagcgcgcc
     3301 gcccagaaat cgcgttctgc agcgcgcgga cggtccgcga atcgttgtta ggcgcgcgtg
     3361 cgcgattctc ggattccgac tccgagcgtg gtctccggcg gcccaccgcg acgaacttcc
     3421 aggctgacga tttcgcgtgc tcgtagctgt tcggcgagca gttggatcgc cttctccgct
     3481 cggacgtcac cacaggtata gaaatccacc gcggcgtagc cggtctccgg ccacgtgtga
     3541 atagacaggt gcgattcttc gacgagtacg agcgcggtta ccccatgtgg ggaaaacggg
     3601 tgaacgagct ctcccacgac gcgcgcaccg gccagctccg cggaggctct caagagctga
     3661 ccgagctgcc gcgcgtcatc gagccgttcg ctcgcgcagc caaagaactc gatcaggtag
     3721 tgttgaccag ttgtacgcac cagagcctga ctagcacgcc ctcaacgttt tccgcgctta
     3781 gcgtgaatct cctgaagttt gcgcaagatc gcccgagcat tgggcctacc cttgaaaacg
     3841 tagagagcag aatgctcgga cgctagaact tcttcgctca ctcgcaactt ggagctgaca
     3901 gcgaacagga gatttggagc cagaccccgc tcgaccagtt cgacgcgccg gaacacggcg
     3961 tcccggctcc agtaccccaa catctcgagg tagatcggag cccctccccg agtgggctcg
     4021 aaaacgagat ccggaacaca gacgccctga ccggccacct cgaagatctc ttcacacggc
     4081 cggcagcgaa attcgctcag ttcggcgaag tcctcgcaca gctcacgcac ctcgtcgcgc
     4141 atgacgtgtg ccgcatcagc gtcaggctgc gctgcttggc tacgatattc gaacaacagc
     4201 ggagtacgct gtttgcccca taacacccgc gcggaaagct ccaacaagtc acacgcctgt
     4261 agggccggta gggtgcacgc tagttccaaa ccatacttgg tgcccgcatc gaacaagctg
     4321 aacggaccgt cgatctcgag gcgatacccc gacggatcgc gacgttccat tcggtagagt
     4381 aggcgtcgaa acttgagctt gtgaaacagg gctcggtacg cgtctggact cttgcaggtg
     4441 acgttcacga cgagactcac ggctcggagc agaatggcct gcacttggcc gcggtcgtac
     4501 tgagtcacga gctccgcagc cgccacctgg ggaaccgcga ggaggcggtg cgccccgcgc
     4561 aaatcggcgt agagcacgtc ttcgagttgc tggacgtcta ggccccgctc cgccgcgacc
     4621 tcctggacca aggcggcgcg gtcgaacgcc acctcactgt gcttgcggcg ctgggccgct
     4681 tttgcgaaca actcgctgcg caacgccgcc ggatcgacga cgggagtttc cccgaactca
     4741 cagtactctt cgacgagctt cttcagccct tcgaacagtt tgcgttcgtt cggggccacg
     4801 ccaatcaagc cgagcgcttc atccaggtcg tcgcgcgtgg cttgaacgtg ggcccgcgcg
     4861 gtctccaaga tctgctccgc gagttccaac gctcgcaggc gcttcttggc ggaaagctcg
     4921 acgaggacca agcgttcacc agtccgtctc acttggacgt gttcggcagc taacattttg
     4981 gaggagcctg gtaggcggcg tgttctcgac ggcgttcgct ggtgaatgcc tccccggtac
     5041 ccgaggacac gagctcgtag agcacagcgc gtttaccgtc tttccgtcgc aggatgcggc
     5101 cgagcctctg cacgtgttca cgcacggagc cgctcccgct aatcacgacg gcgacactgg
     5161 cttccggcac gtccacgcct tcgttcaaca ccttggaggt ggcgatggcg gtgtactcac
     5221 cacgagcaaa gcgttccaag atctcactgc gctctttgac cttggtctga tgagtgatga
     5281 ccgggatcaa gaactgcttg gagacagcat aggccatgcg gttgtcctga gtgaagatca
     5341 ggctgcgctc ctgccgatgc aagtcga
//
LOCUS       DASTKU010000061         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_11893,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000061 DASTKU010000000
VERSION     DASTKU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11893"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..636)
                     /locus_tag="VFQ61_02065"
     CDS             complement(253..636)
                     /locus_tag="VFQ61_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953256.1"
                     /translation="MKALNVRVAKAVNLLLQRRGRLFVDRFHRRVLTTARSVRNTIIY
                     VLANFRKHHASEARRGWLIDPCSSARYFADFFEGNPVDPRFTLFPNPAGSPVVSARTW
                     LLKTAWKRLGPISLHDHPRPREAQG"
     gene            640..840
                     /locus_tag="VFQ61_02070"
     CDS             640..840
                     /locus_tag="VFQ61_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953257.1"
                     /translation="MAECVSMFGFDEQVDVVSLDAEVDDPELALADVVHGAHRVTDCS
                     EYLLVTQAVHDCLQGNEHRLPT"
     gene            complement(1116..2108)
                     /locus_tag="VFQ61_02075"
     CDS             complement(1116..2108)
                     /locus_tag="VFQ61_02075"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9953258.1"
                     /translation="MALTVIVRSGDLIEAPSITFDAPRIVLGRGASSDILLPDPSVSL
                     RHASIRQRGADYVVIDEGSTNGTFVGPVRLAPQAPRVLLSGDLVRVGRVWLELRIESV
                     PPTISPQTRARELARTLVEAALLSDGRGGTRVRVRDGSEAPPELAVGPRERSYIVGRG
                     LGPDLSIDDPELARRHLQIRRHKNGIWVKDLSGKLPVQLGERPLAPNQEEVWTEGTVL
                     HVGKTQLICEDPVRAALAELESCEDEHMLDDDSVDPPPAVRKPAAPVEVPKRAPSRRS
                     AQPEPVLSPGEIPVRQAPPPKPRERMWLLDWLVAFVALAVLSLSLLGIWMLFRV"
     gene            2296..>2848
                     /locus_tag="VFQ61_02080"
     CDS             2296..>2848
                     /locus_tag="VFQ61_02080"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9953259.1"
                     /translation="MTQVQERRDPKNHRVTVSTLVEVCGNVPGTPVFEAESIDLSARG
                     MHLRTAYLPETGTPLVCRFESKGREIVVEGRVAWRRAATRGGEFGVEFTALDSRGVDA
                     LRELCRSGEERPEEDGAELGEPGSRVRLHIEGLGSPMKARVKTSRGSKLQVGSSLEFL
                     KVGRKLELEDLEHGARRGAEIDGI"
BASE COUNT          512 a          806 c         1017 g          513 t
ORIGIN      
        1 cggcgatggt cccggtcccc agcgctcgtc acgccgcact gccggtagac gcaccgatga
       61 cgacgaccag cccgtgacga cttgattcgg tgatggtccc ggtcgccagc gctcgtcgat
      121 tggtgtgcgt tgttgggggc ggcgagcgcg gttttgagag gagatgggtc ggggatggac
      181 tgcgctttgg acgggatatg tgtggggtcg cgctctttac gaggtggccg gcgcattcgt
      241 cagtcggtga ttttaacctt gagcctcgcg cggccgaggg tgatcgtgga gtgagatggg
      301 gccgaggcgt ttccaggcgg ttttgaggag ccacgtgcgc gcggaaacga cgggcgaacc
      361 cgccggattg gggaagaggg tgaagcgcgg gtcaaccggg ttaccttcga agaagtccgc
      421 gaagtatcgc gcggatgagc acgggtcgat gagccagccg cgacgcgctt ccgaagcgtg
      481 gtgtttgcgg aagtttgcca acacgtaaat tattgtattt cgaaccgagc gcgccgtagt
      541 gagtacacga cgatggaagc ggtcgacgaa gaggcgacct cgtcgctgca gcagtagatt
      601 gacggctttg gcgactcgaa cattgagggc cttcatgcca tggctgagtg cgtctcgatg
      661 ttcggcttcg acgagcaggt ggatgtggtc agtttggacg ctgaagtgga tgatccggaa
      721 ctcgcgctgg cggacgtggt tcacggcgcg caccgcgtga cggattgctc ggaatatttg
      781 ctggttacgc aagcagtgca cgactgtctt caaggtaacg agcacaggct gcccacgtag
      841 gcgcgccgga agccgtgagt gtgaaatgcc agaacgaccg ggtcgtttgg gttttcgccc
      901 cgcaccctgg cgtgaaccac cccagttcgt tacgttcctt cgccccgttt ccgaccgaat
      961 gcctagccaa atgcacatgc ggttgccctc cctgcgagaa ccacccgcta aagatgacac
     1021 gctcacggcg gaccacaagc tcagacaaca aaaataattg tctcatccag tcgagtcggg
     1081 aacaccgccg tttcaagggc ccgcgccttt gtggctcaaa cgcggaacaa catccaaatc
     1141 cccagcaggc tcagggacaa taccgcgagc gcgacgaagg ccacgagcca atcgagtaac
     1201 cacatgcgtt cgcgtggttt gggaggaggc gcctggcgaa cgggaatctc acccggtgac
     1261 aagacgggct cgggctgagc cgagcgacgc gagggtgcac gcttcggcac ctcgaccggc
     1321 gcggcgggtt tgcgcacggc cggcggcggg tcgacgctgt catcgtcgag catgtgctcg
     1381 tcttcgcagc tctcgagctc ggccagcgcc gcgcgtaccg ggtcttcaca gatgagttga
     1441 gttttcccaa cgtgaagtac ggtgccctcg gtccacactt cttcttggtt gggcgccagt
     1501 ggtcgctctc cgagctgaac cggtaatttg ccgctcaggt ctttgaccca gatcccgttt
     1561 ttgtgtcggc gaatctgcag gtggcgccgg gcgagttcgg ggtcgtcgat cgagaggtcg
     1621 gggccgaggc cacgaccaac gatgtagctg cgttcacggg ggccaaccgc gagctcgggg
     1681 ggagcttcgg agccgtcgcg tacgcgcacg cgggtgccgc cgcgaccgtc cgaaaggagc
     1741 gccgcttcga cgagggtgcg ggcgagttct cgcgctcggg tctgtgggga gatcgtgggt
     1801 ggtacgctct cgatgcggag ttcgagccag acgcgaccca cccgcaccag gtcgccgctc
     1861 aagagcacgc gcggcgcttg tggcgcgagc cggacgggcc ccacgaaggt gccgttggtg
     1921 ctgccctcgt caatcactac gtagtcggca ccccgctgac gaatggacgc gtggcgcaag
     1981 ctgacgctcg ggtccggcaa cagaatgtcc gagctcgcgc cgcgccccag cacgatgcgg
     2041 ggggcgtcga atgtaatgct cggggcttcg atgaggtcgc cggagcgcac gatcaccgtg
     2101 agcgccattg gtggcgctca tcatgatgaa tgagcggggt gcgggccagg ctgaaatcca
     2161 agccgaggac gcttggcacg cgctattcgc gaagggtgtg tgcggaaagt tggcccagct
     2221 cggcgtggtg ccttaggtcg ggatggctcg aacagcgaaa gccggccgcg ttcggcccag
     2281 gcttcggagg cagtcatgac ccaggtgcaa gaacgccgag acccgaagaa tcaccgtgta
     2341 accgtgtcca cgctggtgga ggtctgcggc aatgtgccgg gcacaccggt tttcgaagca
     2401 gagtcgatcg atctgagcgc tcgcgggatg cacctgcgta cggcgtactt gccggagacg
     2461 ggtacgcctc tggtttgccg cttcgagagc aaaggtcgtg aaatcgtggt cgaggggcgg
     2521 gtggcctggc gacgcgcggc cacacgtggc ggtgagttcg gagtggaatt taccgcactg
     2581 gatagccgcg gcgtggatgc actccgagaa ttgtgccgct cgggcgagga gcgaccggaa
     2641 gaggatgggg ccgagctcgg agagcctggt tcgcgtgttc gcctgcacat cgaggggctc
     2701 ggctcgccga tgaaggcgcg cgtcaagacg agccgcggaa gcaagctgca ggtgggttcg
     2761 agcctcgagt tcttgaaggt cggtcgcaag ctggagctcg aagatttgga gcacggcgcg
     2821 cggcgcggcg cggagatcga cgggatca
//
LOCUS       DASTKU010000062         4247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_11898,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000062 DASTKU010000000
VERSION     DASTKU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11898"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1384)
                     /locus_tag="VFQ61_02085"
     CDS             complement(119..1384)
                     /locus_tag="VFQ61_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9953260.1"
                     /translation="MPAPDIDRVAAAIWRIESAKLIAGLTRLLRRVDLAEEIAQDTFV
                     DALESWRRSGVPENPGGWLRTASRHRALDVLRRTRVMERKNEELEHELVTSQPASFEE
                     ASDDAVKDDLLRLMFVACHPVLAQDSRVALTLRLLGGLSTDEIARAFLVPESTIGQRI
                     YRAKRTLTEKDVPFELPAPAELRARLDSVLEVVYLIFNEGYSATAGDDWMRPGLCQDA
                     LRLGRVLADLMHSSSEVHALVSLMELQASRERARVGPEGEAVLLPDQDRSKWDTLLIR
                     RGLEGLARAERLAADRPGPYLIQAAIAACHARAPNIAETDWTRIASLYAALGHIAPSP
                     IVELNRAVAAGKAFGPAAGLAIADSLRADAALFDYPFLPSVRAALLAELGRFAEALAE
                     VERAATLTKNEREKELFLKRAAAYARQRC"
     gene            complement(1418..1894)
                     /locus_tag="VFQ61_02090"
     CDS             complement(1418..1894)
                     /locus_tag="VFQ61_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9953261.1"
                     /translation="MIRGPNLEGRRKKIAQVSDSAGLERHTSGPTGPEPEMMMRFLVL
                     IGHDEKGGKSMSTEQHQALYTGYQRYEAELKQAGVLLGGEPLQPSASAVRLSTEGGKR
                     KVLDGPFSESKEIIGGYFILEVKSREEAIEWAARCPAAQLGAWSYVEVREIQEIPR"
     gene            complement(1985..2320)
                     /locus_tag="VFQ61_02095"
     CDS             complement(1985..2320)
                     /locus_tag="VFQ61_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953262.1"
                     /translation="MKEASSYPAGHNELLEACRRRERESCHYLAKLELDGAFGSRDER
                     AAAEHFWQACAYNWGPSCSALAYMRAKGVGLSPDAGKAAEFGARACALGHEASCKVLR
                     HGSSDFPPP"
     gene            3194..4060
                     /locus_tag="VFQ61_02100"
     CDS             3194..4060
                     /locus_tag="VFQ61_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953263.1"
                     /translation="MITRRCVQRQFLLRPDDRTTQCFMFCLALAAQRAGVQVVAFLVM
                     SNHYHAIVHDPLGTLPVFLEHLHKLTARALNARWGRWENLWASEQTCATHLVNPDDVL
                     DKVLYTLCNPVVEHLVDRVTDWPGANSYRAMLTGEGIEVERPRDFFRADGTTPEVARL
                     ELIRPPGFEECDLVHWREHLSRLVTTIEERTRAERLQSGAKLLGRKAVLRASAFDTPQ
                     SSEPRRNLRPAVACKDESTRVHVLTQLLAFRRAHEAARQRVVAGELEAMFPYGTYRMR
                     LMGHRCASVPLS"
BASE COUNT          804 a         1422 c         1253 g          768 t
ORIGIN      
        1 tggatacagc cgtgctggga ttgccgtgaa gaatcggaag actgcgatat tcatacccac
       61 gttccgcgat cgtccttttc caattcctcg gcacacgagt tctagttcct agccaccgtc
      121 agcaccgctg acgagcatag gcggccgctc gtttcaaaaa cagctccttt tcacgttcat
      181 tcttggtcag cgttgcggcg cgttcgactt ccgccaaggc ctctgcaaag cggccgagct
      241 ccgccagcag tgccgcccgc acgctgggca gaaacggata gtcgaataaa gccgcgtcgg
      301 cgcgcagcga atcagcgatc gcgaggcccg ccgcgggtcc aaatgccttt cccgcggcta
      361 ctgcgcgatt tagttccacg atgggggagg gcgcaatatg ccccagcgcc gcgtacagac
      421 tggcaatccg ggtccaatcc gtctccgcaa tattgggagc gcgcgcatgg caagcggcga
      481 ttgctgcctg aatcagatag ggtccgggcc tgtccgcggc caggcgttcc gcgcgggcca
      541 aaccctcgag accgcggcga atcaacagcg tatcccattt cgagcgatct tgatccggta
      601 ggagcaccgc ttcgccctcc ggtccgacgc gagcacgctc gcgcgacgcc tgaagttcca
      661 tcaaagagac gagcgcgtgg acctccgagc tcgagtgcat cagatccgcc aacacccgcc
      721 caaggcgtag cgcgtcctga cacagaccgg gtctcatcca atcgtcccca gcggtcgccg
      781 aatagccttc gttgaagatc agataaacga cctcgagtac cgagtccagg cgcgcgcgga
      841 gctcggcggg tgcgggcagc tcgaacggca cgtccttttc ggtcaacgtg cgtttagcgc
      901 gatagatgcg ttgaccgatg gtcgattcag gcaccaagaa cgcacgcgcg atctcatcgg
      961 tgctcaagcc gcccaacagc cgcagcgtca gtgcgacgcg cgagtcttgc gcgagcaccg
     1021 ggtgacacgc tacgaacatc aaccgcaaga gatcgtcctt cacggcatca tcggaagcct
     1081 cctcgaaact ggcgggctgg gacgtcacca gctcatgttc cagctcctcg ttcttgcgtt
     1141 ccatcacacg cgttcgccgc agcacatcca gcgcacggtg tcgcgacgcc gtgcgcaacc
     1201 acccgcccgg gttctcggga actcctgatc ttcgccagct ctccagcgcg tccacgaaag
     1261 tgtcctgcgc gatctcctcc gccaaatcga cacgccgcag aagacgcgtc agcccggcga
     1321 tcagctttgc ggattcaatc cgccaaatgg cagccgcaac gcggtcaatg tcgggagccg
     1381 gcatggctcc cgaccaaaac accacccagc cgtgctgtca ccgaggaatt tcttggatct
     1441 ctcgaacctc gacgtaactc caggccccga gctgcgccgc ggggcagcgc gccgcccact
     1501 cgatcgcttc ctcccgcgac ttcacctcca gtatgaaata gccgccgata atctccttcg
     1561 actcactgaa gggcccatcc agcaccttgc gcttacctcc ttctgtgctc aggcggactg
     1621 cgctcgcgct cggctgaagt ggctccccgc cgagcagcac cccggcctgt ttcaactcgg
     1681 cctcatacct ttggtacccg gtatacagcg cctggtgctg ctccgtgctc atgctcttgc
     1741 cacccttttc gtcatgacca atcagaacta agaaacgcat catcatctcc ggttcggggc
     1801 ccgttggccc actcgtatgg cgttcgagcc ccgccgaatc cgacacctgg gcgatctttt
     1861 tcctgcggcc ctccaaattc ggtccgcgaa tcatccagaa cgccgggcca gcccgcgctt
     1921 ggcaagcgga gcggattgga ttggtgcaag ccctagtcgc gacccgttca gagagacgag
     1981 ccgctcaggg cggcggaaag tccgagctcc catgccgcag caccttgcac gaggcttcgt
     2041 gcccaagtgc acacgctcgg gctccgaatt cggcggcttt gcctgcatca ggggagagtc
     2101 ctacgccttt ggcacgcatg tacgccaacg ccgagcagga cggaccccag ttgtacgcac
     2161 aagcctgcca gaagtgctct gcggcagccc tttcatctcg ggacccaaag gcgccgtcga
     2221 gctccaattt cgcgagatag tgacaacttt cacgttctcg acgccggcat gcttcgagga
     2281 gttcattgtg ccccgcggga taactggaag cctccttcaa cgctactgtg agtgcagcgc
     2341 cccgcccgca agccggcatg tatcgcaaag cgcatgcttt cgctagatac atgcgtccgc
     2401 gaccgagatc tggagcaata cctagcttgc tcgcatcgac agcgagggcc agcccagccg
     2461 cgttgcagcc tgcgtaagac ccctggtcgc aggcactttc ccaactctca aaacggcttc
     2521 aacgtgggtg aggtcaccga gttccaaaga agccacgccc gccagaacgc agccatcggg
     2581 agctcccagc gagcatgcgc gcgtgtggta gcgccgcgct cgctcgaggt tgcgttgtac
     2641 tcccattccg tagtgatagg cctgtcccac gaacctgcac ggctccgcca acccccacga
     2701 acatgcgacc tcttgcttcg caacgaacgc atcgatcgca gtgcgcgtcg cagggagtgg
     2761 aggaggcgcc actccctgcg cgcacccgca gaacatgagc gcggccagaa gcattacgat
     2821 acggttcgat gtcatcaaca gcgtgacgtc cccatatccc agcgacccac gagccattcg
     2881 tcggcgatcc gcatgcaagg gcagtgcggg tggttcgacg cgcagctacg gtgtcgtcaa
     2941 cggacgagct gttcgttgag atcgaatccc cgccatgtgg tggtgggagc taagatgtat
     3001 cgaggtccgt gtcgcgggcg acgccggggc cgcttagagc gacgtcgtgc cacgccagca
     3061 cagcgccacc tctcgccacc ggcggccagg gtgagaaacg cggagcatcg cggcttttcc
     3121 tcttggcacg gacggcgctt tccgcgccgg tgcgatgtgt gccccccgcg agatcatacc
     3181 cggccgatat tggatgatca cccgtcgttg cgtgcagcga cagttcctgt tgcgacccga
     3241 cgatcgcacg actcagtgct tcatgttctg cttggcattg gcggcacaac gcgccggagt
     3301 acaggtggtg gccttcttgg tgatgagcaa ccattaccat gcgatcgtgc acgacccgct
     3361 gggcacacta ccggtgtttc tcgagcacct gcacaagctc acggcgcgcg cgttgaatgc
     3421 gcgctggggt cgctgggaga acctgtgggc atccgagcag acctgcgcga cccacctggt
     3481 caatccggac gatgtcctcg acaaggtgct gtacacgctg tgcaaccccg tcgtcgagca
     3541 cctggtagat cgagtgacgg actggcccgg cgccaattcc taccgcgcga tgctcactgg
     3601 cgagggcatt gaggtggagc gaccgcgcga cttcttccga gcggatggga ccacgccgga
     3661 agtggcgcgg ctcgaactga tccgaccacc cggcttcgaa gaatgcgact tagtgcattg
     3721 gcgggagcac ttgagtcgac ttgtcacaac gattgaggag cgcacccgtg cggagcgcct
     3781 tcagagcgga gccaagctcc tgggcaggaa ggccgtgctt cgcgcatctg cctttgacac
     3841 gccgcagagc tcggaaccac gccgcaacct tcggcccgcg gtggcatgca aagacgagtc
     3901 aactcgcgtg cacgtgctca cgcagctgtt ggcgttccgt cgcgcgcacg aagcggcgcg
     3961 gcagcgagtc gtggcgggcg aactcgaggc gatgtttccg tatggaacct atcggatgcg
     4021 cctgatgggc catcgttgcg cctccgtgcc gttaagttaa gccggatcac gcatggtttg
     4081 acgccgcgtc acacgcggcc caagggagac ctgcggctta cgaaggggag ccatgcggga
     4141 ggacacaggt gtatctatga gaggccccgc gataccctcc ctgcagccaa gttttcacct
     4201 cggagcggct cagcgccacg gttcagcccc cgtggcgctc gcagctt
//
LOCUS       DASTKU010000063         4862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1350532, whole genome shotgun sequence.
ACCESSION   DASTKU010000063 DASTKU010000000
VERSION     DASTKU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350532"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VFQ61_02105"
     CDS             complement(<1..889)
                     /locus_tag="VFQ61_02105"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET9953264.1"
                     /translation="MKLPVNPPVLPMLAKRVDALPKTEGWVFEPKWDGFRALIFRDGS
                     ELLIQSRDEKPLNRYFPELEAPILQQLPDQCVLDGEVVLARGAGLDFDALQQRVHPAA
                     SRVKLLSQQTPASIVFFDLLCEGERDLTQVAFSERRARLEAMLRGARPPLFLTPATRD
                     LVQAEDWFRRFEGAGLDGVMAKPLSGAYEPNKRVMLKVKHERDCDCVVAGFRWHKKGN
                     QEAVGSLLLGLFDDEGTLHHVGVCASFTDKRRRELVEELRPYRERALATHPWRAWAEQ
                     GAALGEDGDQRMPGGKSRWS"
     gene            1158..1541
                     /locus_tag="VFQ61_02110"
     CDS             1158..1541
                     /locus_tag="VFQ61_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953265.1"
                     /translation="MFSKISLRVPFHTPRFSVQLLALCSLLGAMAAGCSGSEGAEDAQ
                     TATPALAQTEQALNCHGSSCDDVLPNNSPCKADMRDTGLGPRSLNSFCGLDRSVLSPT
                     ASRLTRSGDSRIEAEIVRAEIVFRS"
     gene            complement(1491..2111)
                     /locus_tag="VFQ61_02115"
     CDS             complement(1491..2111)
                     /locus_tag="VFQ61_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953266.1"
                     /translation="MGLVDRLLGREEHTDKRSPRDVLAAAEQGRAATPDEMALERYRY
                     MIRTAPPETVEQAHAEAFSKLTPDQRRLVLRELAQASPPSERAAVESTSSEDPAALAR
                     VATRAEVRQPGTMERLLGGGAAGSGMSFGANLLSSFAMGFVGSMVAQSFFSAMNFGGS
                     YAALDDAEAGREDDHPEGNDAGADIDSGDDLASEDDFGSDDFGFDA"
     gene            complement(2286..3806)
                     /locus_tag="VFQ61_02120"
     CDS             complement(2286..3806)
                     /locus_tag="VFQ61_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HET9953267.1"
                     /translation="MPRPPGRAEAIRRLVSTTRLSYMNAELAQSADSGLECSDFGASI
                     RGDSGVGAGRWLDPRRRVRRPGRQQPFRDLLLLGVLALGVAGCNALLGNEPGKKIQSS
                     GGSSNDGGQMHQAGQASGGSPGGVPDPEGGSANSGGGGAEDGGQSGAGSSSGGSGALD
                     PAECEACGTLRLTESFETELFGSTTGGGLAADICPENQVAIGVEAWVNPNYVAGLAVV
                     CGKLRLPKRAGAAQVSQTNTLALRGPAAGSWLLGLCPEGQVLTGIHSSAAPAAETVLL
                     VSLELACASVTWSGQEGSIELHTEAATPVALPFPPRAAATLDDTRRCPADSVVRGLLN
                     RSGAWVDGVGVVCSRPLAARPDGAPCSEDVECAGGRCDGTCSSYECEEPSLCQCRWFD
                     EARYRFCKPLDFTAAAASCSAQGEQLVELTNRAENGWLNSTAKRLSLPVSLWLGADDK
                     AKENVFMWKNRAEVADLGFWPMSGPTGEASKDCLLLGEDGRWRGELCDTQHSFVCQ"
     gene            4035..>4862
                     /locus_tag="VFQ61_02125"
     CDS             4035..>4862
                     /locus_tag="VFQ61_02125"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953268.1"
                     /translation="MLPERVFAPSTAEVTLVLLAGKSHTGYLTRFNPAALDIALALTQ
                     TARADARFAVEQVAYVAFHRGAGDPPPPPSRRGALKIHVSGGKTFAVDPVEPSQPGTL
                     GMYARPAEAQSPYREIFFYNHGINLREVNEPLGAMLLQEGRLAASGLEAGLRAQKDEK
                     RTPIGQILVENKRINQNAVDQAALLQQRRGTRLGEVLVEAGLASAEDIDFALSEQRKR
                     GGKRIGQILIEMNLISEVDLARALARKFQLTFLDLDGCSINLEAVTELPREFIEKHRV
                     "
BASE COUNT          925 a         1642 c         1441 g          854 t
ORIGIN      
        1 ggctccagcg gctcttaccg ccaggcatac gctggtctcc atcttccccc agcgcggccc
       61 cctgctctgc ccaggctcgc caagggtggg tcgcgagggc tcgctcacga tagggccgca
      121 gctcttcgac gagctcacga cgacgcttgt ccgtgaagct cgcgcacaca cccacgtgat
      181 gaagcgtgcc ctcgtcatcg aacaacccca agagtagtga gcccaccgcc tcttgatttc
      241 ccttcttgtg ccaacgaaag ccggccacca cacaatcgca atcgcgctcg tgcttcacct
      301 tgagcatcac gcgcttgttg ggctcgtagg ccccgctcag cggcttggcc atcacgccat
      361 cgagaccggc accttcgaag cgccgaaacc agtcctccgc ctgaaccaaa tcgcgcgtcg
      421 ccggcgtaag gaacaatggc ggtcttgcac cgcgcagcat ggcctccaaa cgcgcgcgac
      481 gctcactgaa ggccacttgc gtcaagtcgc gttctccttc gcagagcaag tcgaagaaaa
      541 cgatggacgc cggagtctgc tgggagagca gcttcacgcg agatgccgcc gggtgaacgc
      601 gctgctgtaa agcgtcgaaa tccaaccctg ccccgcgagc cagcaccacc tcaccatcca
      661 acacgcactg gtccggcagc tgctgcagaa tcggcgcctc cagctccgga aagtagcgat
      721 tcaatggttt ctcgtctcgg ctctggatca agagctcgga accatcccga aaaatcagtg
      781 cacggaaccc gtcccacttc ggctcgaaca cccagccttc ggtcttgggc agggcgtcca
      841 cgcgcttggc gagcattggc aacacaggag gattgacggg gagcttcacg gttccttctt
      901 agtgcttttc gaatccgacg gcccaggttt gcgaagagct agttcgcgaa gaactttagg
      961 ccgagcgaag aggcgggcgg tagtgaattg tttacttcac ccactcagga cgatctgccc
     1021 ggactcctgc tgcgtattcc gatcgctcgc gcggaaggat tagcgcacgc caactgtccc
     1081 gtccgccacc gtgggacagc gggcgcacgc gtcaccgctc gatgcgccca caatctgaag
     1141 agcagatgga gaatcgcatg ttttctaaga tttcactccg cgttccgttc cacacgcctc
     1201 gattcagtgt tcagctgcta gctctttgca gcttgctcgg ggcgatggcc gccggctgtt
     1261 ccggcagtga gggagccgaa gacgcgcaga ccgcaacgcc agcgctcgcg caaacagagc
     1321 aggcattgaa ttgtcacggc tctagttgcg acgacgtatt acccaataac tcgccttgca
     1381 aagcggacat gcgggatacg ggcctgggtc cgcgctccct gaatagcttt tgcggcctcg
     1441 atcgaagcgt cttgtccccg accgcgtcga gactgacgcg gtcaggggat tcacgcatcg
     1501 aagccgaaat cgtccgagcc gaaatcgtct tcagaagcta aatcgtcacc ggagtcaatg
     1561 tcggctccag catcgttgcc ttccggatgg tcgtcttcgc ggcccgcctc ggcgtcatcc
     1621 agggctgcat agctgccacc aaagttcatc gccgagaaga agctctgggc caccatgctg
     1681 cccacgaagc ccatcgcaaa gctcgacagc aaattggcgc caaaactcat accgctgcca
     1741 gcggcgccac cgcccagcaa acgctccatc gttccgggct ggcgtacttc ggcgcgtgtg
     1801 gcaactcggg ccaaagctgc tggatcctca ctagaagtgg actccaccgc ggcccgctcc
     1861 gacggcgggg acgcttgcgc cagctcacgc agtaccaagc ggcgctggtc cggagtcagc
     1921 ttggaaaacg cttccgcgtg cgcttgctcg acggtctccg gaggggcggt tcggatcatg
     1981 tatcgatagc gttctagcgc catctcgtca ggagtcgccg cgcggccctg ctctgccgct
     2041 gcgagcacgt ctcgagggga gcgtttgtcc gtgtgttctt cgcggcccaa taggcgatca
     2101 acgagaccca tgacgagcta gtcctttcct ggcgttttgc cgtgccattc gtcggtgcaa
     2161 catctgcgcc gtgccgcgat ccagccaagt tccggctaga cagtgaggca gcgatgagac
     2221 tcgcgggtca tctctgccgg acaccgcacc acgagcagaa ggagctgacg agcgaaaagg
     2281 ccccgctact ggcagacgaa cgagtgttgc gtgtcacaaa gctcaccgcg ccaacgccca
     2341 tcctcaccca acaacaggca gtccttggac gcttcgcccg tgggcccgct catgggccag
     2401 aacccgagat cggcgacttc ggctcggttc ttccacatga atacgttttc tttggcctta
     2461 tcgtcggcac ccagccacag cgacacgggc aaactcaatc gcttcgccgt gctgttcagc
     2521 cagccattct cggcccgatt cgtgagctcg accagctgct caccttgggc cgagcagctg
     2581 gcggcggccg cggtgaagtc taggggtttg cagaatcgat atctagcttc atcgaaccac
     2641 cggcattggc agagagacgg ctcctcgcac tcgtagctgc tgcaggtgcc atcacaccgt
     2701 ccacctgcac actcgacgtc ctcggagcaa ggcgctccgt ctggtcgcgc ggcgagcggc
     2761 cggctacaca cgacaccgac gccatctacc cacgcgccac tgcgattcaa aagccctcgc
     2821 acgacgctgt ccgctggaca ccgccgcgta tcgtccaacg tcgcggccgc gcggggcgga
     2881 aacggaagtg cgaccggagt agccgcttcg gtgtggagct cgatcgaacc ttcttgccct
     2941 gaccacgtaa ctgaagcgca agccagctcc agcgaaacca gcagcacggt ctccgccgcg
     3001 ggtgcggcgc tcgagtgaat gccggtcagg acctgaccct cgggacacag gccgagcagc
     3061 cacgagcctg ccgcggggcc cctcagcgcg agcgtattcg tttgcgaaac ctgcgctgcg
     3121 cccgcacgct tcggcaaacg tagcttgccg cacaccaccg ccaaaccagc aacgtagttg
     3181 gggttcaccc aagcctcgac gccgatcgcg acttggtttt ccgggcaaat gtcggcggcc
     3241 aagccgccgc cggtggtact gccgaacaac tcggtttcga aggactcagt caaccgtaga
     3301 gtcccgcatg cctcgcattc agcggggtcc aaagcacctg aacctcccga ggatgaacct
     3361 gcgcccgatt gtccgccgtc ttctgcgccg cctcccccag agttggccga gccgccctcg
     3421 ggatcaggaa ctccgcccgg ggagccgccg ctcgcctgac cggcttggtg catctgcccg
     3481 ccgtcgttgc tggagccccc gctcgactgg attttctttc ccggctcgtt tccgagcagc
     3541 gcgttgcagc ccgccacccc gagcgcgagg acgccgagca gcagtagatc ccgaaaaggc
     3601 tgctgacgac cgggccgcct gactctccgg cgcgggtcga gccacctgcc ggcgccaacg
     3661 ccggaatcgc cgcggatgct cgcgccaaag tcactacatt cgaggcctga atctgctgat
     3721 tgcgccaact cggcgttcat ataggacaag cgcgtagtcg aaaccagtcg tcgaatggct
     3781 tccgcgcgcc ctggtgggcg aggcactctc gtgctaccgg cggttcgaat tcacacgaga
     3841 gtgcctcagg tcacgagcgt gttacgagca ccgtgccact ttcgggagca ctttcgggcg
     3901 tgcttttcga ctgtgcagcg agggagagcg cgaaacacat ttgcagaggt tcgcgtcagc
     3961 gcccggccca cttggctagc tacgcggcag tgggcttcag tgagaggcct cgcgtccgtt
     4021 gatccgccac cctcatgctc ccggagcgcg tgttcgcacc cagcacagcg gaagtaacgc
     4081 tcgtgttgct cgccggtaag tcccatacgg gctacctcac tcgattcaac cccgccgcgc
     4141 tcgacatcgc gctcgcgcta acccagacgg cccgcgccga cgcgcgcttc gccgtagaac
     4201 aggtggcgta cgtagcgttc catcggggag ccggagaccc gccacctccg ccgagccggc
     4261 gcggcgccct gaagattcac gtgagcggcg gtaagacctt cgcagtggat ccggtcgagc
     4321 cgagtcagcc aggcacgctc ggcatgtacg cacgcccggc ggaagcccag agcccttatc
     4381 gcgagatctt cttttacaac cacggcatca acctgcgcga ggtgaacgag ccactcggcg
     4441 ctatgctcct gcaggaagga cggctcgccg catcggggct cgaggcaggt ctgcgtgccc
     4501 agaaagacga gaagcgcacg ccgattgggc agattctcgt cgagaacaag cgcatcaacc
     4561 agaacgcggt cgatcaggcg gccctgctgc agcagcgacg cggcacccgg ctcggggagg
     4621 tgctggtgga ggccggattg gcgagcgccg aagatatcga ctttgcgttg agcgagcagc
     4681 gcaaacgcgg tggtaagcgg atcggccaaa tcctgatcga gatgaacctg atctcggagg
     4741 tagacctcgc gcgcgcactg gcccgcaagt tccaactcac gttcttggat ttggacggct
     4801 gcagtatcaa cctcgaagcc gtgaccgagc tcccgcgtga gttcatcgag aagcaccgcg
     4861 tg
//
LOCUS       DASTKU010000064        10570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1795916, whole genome shotgun sequence.
ACCESSION   DASTKU010000064 DASTKU010000000
VERSION     DASTKU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795916"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VFQ61_02130"
     CDS             complement(<1..952)
                     /locus_tag="VFQ61_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9953269.1"
                     /translation="MSAAAAALRFNHPNPPCRPDRARRRARSLARASAGLGVLLVLEA
                     PMSLKRGVPSILARPKALALPVPHGAATMLVMPSFELSEQQQALVRAARDFTRKEIIP
                     EASRLDEAGTFPREICHKAWEAGLLNCEIPEAYGGVGLSCFEHCLIIEEIAYGCMGVA
                     TTMDGNLLGSMPILIAGTEDQKRRYFGRLLESPIFAAYCCSEPDAGSDVAGLKSSYRK
                     DGDGYILTGQKRWITNGGVADFYTVFARQQGTERHAGISCFVVDRNLPGVSTGKKENK
                     MGQRASDTSDVLFEDVRLGPECLVGREGEGFKLAMRTFDRT"
     gene            complement(949..1347)
                     /locus_tag="VFQ61_02135"
     CDS             complement(949..1347)
                     /locus_tag="VFQ61_02135"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HET9953270.1"
                     /translation="MFLSKHFLGLPPNEEEDIRRNLDNVLSTKRGAGYFLSNFGLSDV
                     AFRTPEEAVTALSRELEENIRLFEPRVELLKINEVYDDDGRRVRLVAVLRRRGTGATL
                     RLLVNLADGSFDWSAAGGARDGEEPDPLGR"
     gene            complement(1392..2489)
                     /locus_tag="VFQ61_02140"
     CDS             complement(1392..2489)
                     /locus_tag="VFQ61_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953271.1"
                     /translation="MDDLEGLDSPTDLTELERRIVAEAQRFELAPLLAVLQDAEYSRD
                     DVLFESEPEGRSPGVVRAVRFVKRPIRCAIVTVQFGLLGDNTLLPSYFFRLLEQSPNP
                     ARFYDFLRFFDHKLIENFFAALHPELVDETTTQSESVFVDYRNLVATFLQMATPGSLS
                     TLHWLFQLYFPEFGVRVSRQAFADATDSHACEVGRSSLDGSGVLGRTYVTEMAGFLVD
                     LVVEDEVDSSGREQADIVLWRLRNRVLPRLAEFRLALTVRLVVLWHASAAHVDDPRAM
                     DKSFLGYNRLRGNRPKPEVRHTTLMYRGRTHEVGHPPARESTPNTSPTLRAKADLVES
                     LAPLSVGRTTSRLPTSSVPPSSSRLPTWPIR"
     gene            complement(2535..3491)
                     /locus_tag="VFQ61_02145"
     CDS             complement(2535..3491)
                     /locus_tag="VFQ61_02145"
                     /inference="COORDINATES: protein motif:HMM:NF041244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein IglI family
                     protein"
                     /protein_id="HET9953272.1"
                     /translation="MAIELRVLCEPLSSPTQLPEDLDESLDKIDRLAEKQEFTDAARA
                     VAELCEQGLFDIRPLAYWLYEEFRERGFAGLADVFRALENLLGPSLAVLGPERRKSEF
                     INRRLTWLFETIAANLEYHEKFSTPEWESLRAGYSEEMQDLLQQSGARIGQLLSSQPE
                     PAPLDAGRALAQLLSRIRLEPKNEPANVETPMGAASAGPSPGNTSVEAKLAGPTAGVA
                     DRMELPVAPPFVALCRKLQAFELLVQKQDWRKAAVLSDDIAASLEHFDPLVHFPALFA
                     PYVALLSKHAQRLYDLEQERQTPTWRALARHARVDLDSFVDT"
     gene            complement(3529..3912)
                     /locus_tag="VFQ61_02150"
     CDS             complement(3529..3912)
                     /locus_tag="VFQ61_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4280 domain-containing protein"
                     /protein_id="HET9953273.1"
                     /translation="MGQLAAAGAILKCSMGAAPSSLVVLPKNRVLAGPPVANIMDQVP
                     FLNVLPFGMCQSMANPVVAAATAAALGALTPMPCIPAIAGPWLPGLPNVLVGNLPAVD
                     SGCQLMCAFGGAISISAPGQFQVSA"
     gene            complement(3922..5685)
                     /locus_tag="VFQ61_02155"
     CDS             complement(3922..5685)
                     /locus_tag="VFQ61_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953274.1"
                     /translation="MREVQLTAPPAESKLDVRAILGALRSRCTSEAFFERWLPQAYAD
                     PAAFERALYEHLATRRAGLKTRAGEPVDFYYDCVVAHLGRGRPALIGLDRGVVRSLTF
                     EALHEACSALLVAWRRAGVTAGQTLYLGLELGRDYLVALMTALRAGLIVCPVPPYGPS
                     FVRYRLQQLRTDWAILPGRMAELLIALRSGDEALTTESLPLSADRGFTSPDTYSYAPD
                     ESALRCLAIHGEEAFLPCDISARRVHAALVRDSSIVLALDQDDVLAAPALDALVHQPM
                     LVLSAMLAGAAFVALPLEDVLHAPERLGEVGVTVLGVGTSLRRVIEARGAWPFSTVRA
                     WFRCLTSELDWTSWDNLSRWSAKLGIPSFNVAFANTAPGGLLFGPARAEPLNFSAWPV
                     PGPPFVLTEVLGGSLEALADTGVYSPMNGDEPVPGIPRLTLTRQANNYLSSGALELGP
                     EAQRYPIEEASRVLQHVPGVRYASVVVSAGRPINSAQITWLLFTDVQPDDPTRPVIAD
                     VRRFFEREMGTPFLPQRIEMFSLRPRTLPPKTGTETGSVIHGWCRSQYLSGSLTAKER
                     SEVFRLLSRLSYLFDSELSED"
     gene            complement(5682..6197)
                     /locus_tag="VFQ61_02160"
     CDS             complement(5682..6197)
                     /locus_tag="VFQ61_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953275.1"
                     /translation="MTLPAAPLAFTGTGCSRRLGSSRRATLLGVLVLLGIGVGCRSSK
                     PSQAPEPSKARCALRVQSEPVTNGGLVLHMLVRRLDPKALGVAESYQEAAARVAAEVR
                     DESVVLVRPIIPGQALFIPLEVVKEERLIACFFFTHPGRNWRVELPSPLPPALEIRLG
                     QNEVKQVVSLP"
     gene            complement(6194..10165)
                     /locus_tag="VFQ61_02165"
     CDS             complement(6194..10165)
                     /locus_tag="VFQ61_02165"
                     /inference="COORDINATES: protein motif:HMM:NF025689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HET9953276.1"
                     /translation="MAAPAIASALPVVPVASALVPVPIASVLAVPIGSGLPSRVAPAP
                     ATSAGPGVLVLDRLRGEASSSAGSYGPWLLIALGLAVPVAVVLGKRAWDYWKKRGAQK
                     RALGPNVLKGVGEPRIPEIESRALRRAWLRFARRLPGEYRRSILNFEQFVLMGLAGSG
                     KSRLLETHTDYHYQMRQVIGERPVDAELPVVLSSSAVIMELPERYLLDETLTSKRALE
                     RLWRPLYSKKAPSVVLTVDAQWLLSSPRDALMDLARSVRARVNQLSAIRKKEVELRIA
                     LTHLDRLTGATEAADFWSRIGMSSRIPLLEAEPVRDALRIWEDELHLQLPRALGVCSA
                     EQYRRVIAFCRQLPKLVEPLGTLAAALYSTDVMSLSPARGGIYFCLTNVKGANPLRGA
                     LDAGPGPSPVRRHWIGAAAVTSASVVYMSFAYAEQRELWIPAADAVASYEVSPELVGS
                     VRERKLREAMVDFAVRHRDLVHRLPNFHGKARREIAESLSSKLRESLLIPRLKEVAQH
                     GILGPDGMTLRWRRAIYYLGLIHSFERDRIGVLSRLDVWRSMTGLPRDVIEDYLVSTA
                     HPYIDPIVFKLGAGANDDRDRAPYWDQLADMVLKAMVDGVLRPEELADMQRLAAEMQQ
                     SLARFDYDDVTLGVFQNLDDAADEGGERYGRSAPHLRDAYAPHFNEIIRSVEAADLQR
                     QSGELRRLLEAVAAARIHVPECKLLRDLIDRLELLKHVAAKKDTQAIHLKLAEKEFTI
                     ELEKWHEILRDSRAALLVDSFVSAAKHRDSAFFTPALEAELLPVVWNPLGSEDTIFGG
                     TARLAGPYTKAAFDTHVRKEITRLAAALSNIHVPAGDRNALIELLRKELNGYATRYQA
                     EARRFLGSYELHVNSVQMLRVALGQIAAERSTFDDFVHELARNTDLEPDADAGAASQT
                     SSGQEKSEPTAPGRTTPSDAAAEPAPGSPEFPDKPDKQSAGADVLSLLEPVYAQLREF
                     KPWQAAVLPSELDKYKEIAKQLLLEIDAKAEGKADPAAKPAPAELSNELSAVGRVTLT
                     SLNDTRNSYAEMVRAWASSLGLSREQRAPFLAPFQELTSMGHADIEQGVARVWYREMA
                     PLVKSVASKFPFDRSAEEPATPDELAAVFHPKDGKFFALYRSYVEPVLPSDGKRNANQ
                     VRLPSDLWATLDAANAVQSRLWDEKGEPRRIVLKMTPVPFARGQSERWIPTLVYLSSG
                     GVSLVNFNQTPGPTVIPVDWTKDEPAQLGIQLTESRTGQQTSPAPLLVEGPYWRIFRL
                     LHAAKATRVRSPSAGTLYEWQLNTGDGSRDSVNVGVIVQGEPWQGLTLPRINLTGSRV
                     VGG"
     gene            complement(10168..>10570)
                     /locus_tag="VFQ61_02170"
     CDS             complement(10168..>10570)
                     /locus_tag="VFQ61_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953277.1"
                     /translation="IELLLERGRPEVPYVEFARKEGLLYCDIDKNVKRAKHVFLLIQK
                     PSVAAKVELSRTKLSSPSRLHAVHERSLTGIPISRIERPPFAQSLSSAVEIYGLSYGQ
                     EWDFAVSEGRVVMFDSPVLDGCRVYLYSRVE"
BASE COUNT         2105 a         3402 c         3206 g         1857 t
ORIGIN      
        1 gagtacgatc gaaggtgcgc atggcgagct tgaagccctc gccttctctt cccaccagac
       61 actccggccc aagccgcacg tcttcgaaca gcacatcgct ggtgtccgaa gcacgctgac
      121 ccatcttgtt ttctttcttg ccagtggaga cgccgggcag gttgcgatcc accacgaagc
      181 agctaatgcc cgcgtgccgc tccgtcccct gctgccgcgc aaacacggtg tagaagtcag
      241 ccactcctcc gttggtgatc cagcgcttct gcccggttag aatgtagcca tcaccatcct
      301 tgcgatagga gctcttgagc cccgcgacgt ccgagcccgc atccggctcg gaacagcagt
      361 aggccgcgaa aatcgggctc tccagcagcc gcccgaaata gcgccgcttc tgatcctcgg
      421 taccggcaat caaaattggc atgctgccca gcaggttgcc gtccatcgtc gtggccacgc
      481 ccatgcaacc ataggcgatc tcttcgatga tgaggcaatg ctcgaagcac gacaggccga
      541 cgccgccgta agcctcgggg atctcgcagt tcaatagacc tgcttcccag gccttgtggc
      601 agatctcgcg ggggaaggtc ccggcttcat cgagacgtga agcttcgggg atgatttcct
      661 tgcgcgtgaa gtcccgggcc gcacgcacca atgcttgttg ctgctcgctc agttcgaagc
      721 tcggcatcac gagcattgtc gcagccccgt gcggcacagg caatgccaaa gccttggggc
      781 gggcgaggat cgacggaact ccgcgcttca ggctcatcgg agcctcgagc acgagcaaca
      841 caccgagacc cgcggacgcc cgcgcgagac tccgtgcgcg acgccgcgca cgatcgggac
      901 ggcaaggtgg gttcgggtgg ttgaagcgaa gcgctgccgc cgcggcactc atcgcccgag
      961 cggatccggc tcctccccgt ctcgtgctcc acccgcagca gaccagtcga agctaccgtc
     1021 cgcgagattg accagcagcc gcagcgttgc tcccgtgcca cggcgccgca gcaccgccac
     1081 gagccgcacg cgacgaccgt cgtcatcgta gacctcgttg atcttcagca actcgacgcg
     1141 tggctcgaac aggcggatgt tctcttcgag ttcgcggctc agcgccgtga ccgcctcctc
     1201 gggagtacgg aaagctacgt cgctgagccc gaagttcgac aggaagtagc cggcgccgcg
     1261 cttggtggag agtacgttgt ccaggttgcg ccggatgtct tcctcttcat ttgggggcaa
     1321 acccagaaag tgtttactga ggaacatgga gtacgaagct cctcgcgcat tcgcggggaa
     1381 tgcagctcgt ttcaacgaat gggccaagtc ggaagtctcg agctagacgg cggcactgaa
     1441 ctggtgggga gccgcgacgt cgtacggccc acgctgagag gcgccaagct ctcgaccaga
     1501 tcggcttttg ctcgcagggt gggcgacgtg tttggagtgc tctctcgagc tgggggatgc
     1561 cccacctcgt gagtccggcc acggtacatc aacgtggtgt gacgcacctc cggtttgggc
     1621 cggtttccgc gcaggcgatt gtaccccaga aagctcttgt ccatggcccg gggatcgtcc
     1681 acgtgggccg cgctcgcgtg ccaaagtact accaggcgaa ccgtgagcgc cagccgaaac
     1741 tcggcgagcc gcggcaaaac gcggttcctc aagcgccaga gcacgatatc cgcttgctct
     1801 cgaccgctag agtcgacctc gtcctcgacc accaagtcca ccaggaatcc agccatttct
     1861 gtgacataag tgcggccgag cacgccgctg ccgtcgaggg aagagcggcc cacctcgcag
     1921 gcgtggctgt cggtggcatc ggcgaacgct tgccggctca ccctcacgcc aaactcaggg
     1981 aagtaaagct ggaacaacca gtgcagggtg gacagcgagc ccggtgtcgc catctgcaga
     2041 aaggtcgcaa ctaggtttcg atagtcgacg aagaccgact cgctctgagt cgttgtttcg
     2101 tccaccagct cggggtgcag cgcagcaaag aaattctcga tcagcttgtg gtcgaaaaac
     2161 cgcaggaaat cgtagaagcg cgccggattg gggctctgct cgagcaaacg gaagaagtag
     2221 cttgggagca gggtgttgtc gccgagtagc ccgaactgca ccgtcacgat cgcgcaacgg
     2281 atggggcgct tgacgaagcg cacggccctg accactccgg gtgagcgccc ctcgggttcg
     2341 ctctcgaaca agacgtcatc acgggaatac tctgcgtctt gcaataccgc cagcaacgga
     2401 gcgagctcga agcgctgagc ttccgcaacg atgcggcgct cgagttcggt cagatcggtt
     2461 ggtgaatcga gcccttccag atcatccatg gctgactctc gcagtgactc agctatcgct
     2521 ctcgatctcg ggactcaagt atccacgaag ctgtcgagat cgactcgggc gtgacgcgcg
     2581 agtgcgcgcc aagtaggggt ttgccgctcc tgctcgaggt cgtacaagcg ttgcgcgtgc
     2641 ttgctgagca acgcgacgta tggcgcgaac agcgccggga agtgaaccaa cgggtcgaag
     2701 tgctctaggc ttgccgcgat gtcatccgag aggacggccg ctttccgcca atcctgcttt
     2761 tgaaccaaca gctcgaaagc ctgcagcttg cgacacaagg ccacgaaggg cggcgccacc
     2821 ggaagctcca ttcgatctgc gacgcccgct gtcggaccgg ctagctttgc ttcgaccgag
     2881 gtgtttcccg gggagggccc cgcgctcgcg gcccccatgg gagtctctac gttagcgggc
     2941 tcgttcttgg gttcgagacg aatgcgcgag agcaactggg ccagagctcg cccggcatcc
     3001 agaggggcgg gctcgggctg gctagaaagc agttggccaa tccgtgctcc actctgttgg
     3061 agcaagtctt gcatttcctc ggagtagccg gcgcgcaggc tctcccattc tggtgtcgag
     3121 aacttctcgt gatattcgag gttagccgcg atggtttcga acaaccacgt caacctacga
     3181 ttgatgaact cgcttttgcg gcgctcgggc ccgagcaccg cgagacttgg ccccagtaag
     3241 ttctcgagag cgcggaacac gtccgcgaga ccggcgaagc cgcgttcacg aaactcctcg
     3301 tacaaccaat acgccaacgg gcggatatcg aataacccct gctcgcagag ctcggcgacg
     3361 gctcgcgccg catccgtaaa ctcctgcttt tccgccagtc gatcgatctt atcgaggctc
     3421 tcgtccagat cctccggcag ctgcgtcggg ctcgagaggg gctcgcacag gactcgtagt
     3481 tcaatggcca tgattcactc gtggagcttg cggagctata cgccggtttc aggcgctgac
     3541 ctgaaattgg ccaggcgccg agatcgagat tgcgccaccg aaggcgcaca tcaattgaca
     3601 gccagagtcg acggcaggca agttccccac caatacgttg ggtaatccgg gcagccaagg
     3661 accggcgatg gcgggaatgc agggcatggg agtgagcgcc ccgagcgccg cggccgtcgc
     3721 ggccgctacc accgggttgg ccatgctttg acacatcccg aacggaagca cattcaggaa
     3781 cggaacttga tccatgatgt tcgccacggg cgggccagcc agcacccggt tcttgggcaa
     3841 caccaccaac gaagaaggtg ctgcccccat tgaacacttc aagatcgcgc ccgcggccgc
     3901 taattgtccc atctgatctc cttagtcttc cgatagctcg gaatcgaaga ggtatgacaa
     3961 gcgagagagc agccggaaca cctcggatcg ctctttggcc gtcagtgaac ccgacaaata
     4021 ctgagagcgg caccagccat ggataaccga acccgtttcg gtgccggtct tcggcggcag
     4081 ggtgcgtggt cgcaacgaga acatctcgat acgctgcggc aagaaaggag ttcccatctc
     4141 acgctcgaaa aagcgtcgga cgtcggcgat gaccggtcga gttggatcat cgggctgcac
     4201 gtcagtgaag agtaaccagg ttatctgggc cgagttgatc ggacggcccg cggaaacgac
     4261 cacactcgcg taacgcacgc ccggaacgtg ctgcagcacg cgactcgcct cttcgatcgg
     4321 gtaacgttgg gcttcgggtc ctagctctag ggctccgcta ctcaggtagt tattggcttg
     4381 ccgagtcagt gtgagccgag gaataccagg caccggctca tccccattca ttggcgagta
     4441 caccccagtg tccgccaaag cttccaaact gccgccgaga acctcggtca agacaaacgg
     4501 cggtccgggg acgggccaag cggagaaatt cagcggttct gcgcgcgcgg gcccgaatag
     4561 caaacctccc ggcgcggtgt tggcaaacgc aacattgaat gaaggaatgc ccagcttggc
     4621 ggaccagcgc gataagttat cccaactggt ccagtccaat tcggaggtca ggcaacgaaa
     4681 ccaggcccga accgttgaaa acggccaagc tcccctagcc tcgataactc gacgcaagga
     4741 agtcccgacg ccgagcaccg tgacgccgac ctcgcctaag cgctccgggg cgtgcaaaac
     4801 gtcttcgagc ggcagagcga cgaaagctgc accggccagc atggccgaaa gcaccagcat
     4861 gggttggtgg accaaagcgt cgagggccgg agccgcgagc acgtcatcct gatccaaagc
     4921 gagcacgatg ctggagtcgc gcacgagcgc tgcgtgcacg cgacgcgcgc tgatgtcgca
     4981 aggaagaaac gcctcttcgc cgtgaatggc caagcatctg agcgcgctct cgtctggcgc
     5041 gtaggagtaa gtatccgggc tagtgaagcc gcggtcggca ctcaacggca acgattcagt
     5101 ggtcaaagcc tcgtccccgg agcgcagcgc aatcagcagc tccgccatac gacccggcag
     5161 aatggcccaa tccgtgcgaa gctgctggag acgatagcgg acgaagctcg gtccgtaagg
     5221 cggaaccggg cagacgatca gccccgcacg caacgccgtc atcaacgcca ccaggtaatc
     5281 tcgcccgagc tcgagcccca gatataaagt ttgacctgcg gttactcccg cgcgccgcca
     5341 cgcgaccaag agcgcggagc acgcctcgtg aagagcctcg aacgtgagcg acctgacgac
     5401 tccccggtcg agtccaatca gtgccgggcg cccacgtccc aaatgcgcaa ccacgcaatc
     5461 gtagtagaag tccaccggct cacccgctcg ggttttgagg cccgcgcgac gggttgccag
     5521 atgttcgtac aaggcgcgct cgaaggcggc tggatcggcg taagcctgcg gcaaccagcg
     5581 ctcgaagaat gcctccgagg tacaccggct tcgaagcgcc cccagaatgg cccgcacatc
     5641 cagcttggac tcggcaggcg gagccgtcaa ttgcacctcc ctcacggcaa actcaccact
     5701 tgcttcacct cgttttgacc gaggcggatc tcgagcgcgg gcgggagtgg actcggcagc
     5761 tctactctcc agtttcggcc cgggtgggtg aagaagaaac aagcaatcaa acgctcttcc
     5821 ttcaccacct ccaacgggat gaacagcgct tggccgggga tgatggggcg aaccaatacc
     5881 acgctctcgt cgcgcacttc ggccgctacg cgcgccgcag cctcttggta tgattcggca
     5941 acgcccagtg ccttcgggtc gagccgacgc accaacatgt gcagaactag cccgccattg
     6001 gtgaccggct cgctctgtac gcgcagcgcg cagcgcgcct tggagggttc gggagcctgg
     6061 ctgggtttgc tcgatcgaca gccgactccg attccgagca gcaccagaac cccgagcaac
     6121 gtcgcacggc gcgaagatcc gaggcggcga ctacacccgg ttccggtgaa agcaagcggc
     6181 gccgcgggca gcgtcatccg cccacgaccc tcgagcccgt tagattgatg cgcggcaggg
     6241 tcaagccttg ccaaggctca ccctgcacta tgacacccac gttgacgctg tctcggctcc
     6301 catccccagt gttcagctgc cattcataga gcgtacccgc ggagggagaa cgtacgcggg
     6361 tggctttggc ggcatggagc agccggaaga tacgccagta gggcccctcc accagcaggg
     6421 gggccggcga cgtttgttgg cccgttcgtg actcggtgag ctgaatcccg agctgcgcgg
     6481 gctcgtcctt ggtccagtcg acggggatga cggtgggccc cggggtctgg ttgaaattga
     6541 cgagcgatac ccctcctgag ctcaggtaaa ccagggtcgg gatccaacgc tcactctgcc
     6601 cacgagcgaa gggcactggc gtcattttga gcacaattcg tcgcggctcg cccttctcgt
     6661 cccagagccg cgactgcacg gcgttggccg cgtcgagcgt ggcccagagg tcagacggca
     6721 gtcgaacctg gttcgcgttt cgtttgccgt cgctcggcaa tacgggctcg acgtagcttc
     6781 gataaagggc aaagaacttg ccgtctttcg ggtgaaatac cgcggccagc tcgtcgggag
     6841 tggccggctc ttccgcgctg cgatcgaacg gaaacttcga cgctaccgat ttgacgagcg
     6901 gcgccatctc tcgataccaa acccgggcta cgccctgttc gatatccgca tgacccatac
     6961 tcgtcaattc ttgaaaaggc gcgagaaacg gggctcgctg ctcccgactc agccctagcg
     7021 agctcgccca cgcccgcacc atctcggcgt atgagttgcg agtgtcattc agcgaagtca
     7081 gcgtaacccg acccacggcc gagagctcgt tcgagagctc cgcgggtgcg ggctttgcag
     7141 cgggatccgc cttgccttcg gctttggcgt cgatctccaa aagcaactgt ttggcgatct
     7201 ctttgtactt gtcgagttcg gagggcaata ccgccgcttg ccaaggcttg aattcccgca
     7261 actgagcgta cacgggttcc agcaaactca gcacgtcggc gccggccgac tgcttgtctg
     7321 gtttatccgg aaactccggt gagcccggcg cgggctctgc ggctgcatca ctaggcgtcg
     7381 tgcgaccggg tgctgttggc tctgacttct cctgacctga actcgtctga gaggcggccc
     7441 ccgcatcagc gtccggctca aggtcggtat tgcgcgcgag ctcgtgcacg aaatcgtcga
     7501 acgtcgaccg ctcggcggca atttgaccga gggccacccg tagcatttgg acggagttca
     7561 cgtgcagctc gtaactcccc aggaagcgcc gcgcttccgc ttgatagcgt gtggcgtaac
     7621 cgttgagctc cttgcggagc aactcaatca aagcattccg atcgcccgcg ggcacatgaa
     7681 tattcgagag tgcagccgcg agccgagtaa tctccttgcg aacgtgggta tcgaacgcag
     7741 ctttcgtata gggccccgcg agtcgcgccg tgccaccgaa gatcgtgtcc tcggagccga
     7801 gcggattcca aactacgggc aacaactccg cttcgagagc gggcgtaaaa aacgcgctat
     7861 ctcgatgttt tgccgcgctc acgaagctat cgaccaacaa agccgcccga ctgtcgcgca
     7921 gaatttcgtg ccacttttcg agctcgatgg tgaactcttt ctcggccaat ttgaggtgga
     7981 tggcttgagt atccttcttg gccgcgacgt gtttcagtag ctctagtcgg tcgatcagat
     8041 cgcgaagcaa cttgcactcg ggcacgtgga tccgagccgc cgcgactgcc tccaacaagc
     8101 ggcgcaattc tccggattgg cgctgcagat cggcggcctc caccgagcgg atgatctcat
     8161 tgaaatgggg cgcgtaagcg tcgcgtaagt gcggagcgct cctgccgtag cgttctccgc
     8221 cttcgtcggc agcatcatcc aggttctgaa ataccccgag tgtcacgtcg tcgtagtcga
     8281 agcgagccag tgactgctgc atctccgcgg ccagccgctg catgtcggcg agttcctccg
     8341 ggcgaagcac gccgtcaacc atggccttca gcaccatgtc cgcgagctgg tcccaatagg
     8401 gagcccggtc gcggtcgtcg tttgcgcccg cacccagttt gaacacaatc ggatcgatgt
     8461 aaggatgtgc ggtgctgacc aagtagtctt cgatcacgtc tcgaggtaac cctgtcatcg
     8521 accgccagac atcgagtctc gaaaggacac ctatcctatc tcgctcgaag ctgtggatga
     8581 ggccgaggta gtaaatggct cggcgccagc gcagcgtcat accgtccgga cccaagatgc
     8641 catgctgggc cacctccttg agacgcggaa tgagcagcga ctcgcgaagc ttgctggaca
     8701 atgactcggc gatttcccgg cgcgccttac cgtggaagtt cgggagccga tgcaccaagt
     8761 cccgatggcg tactgcgaag tccaccatgg cctcgcgcag cttgcgttcc cgcacgctgc
     8821 ccaccagttc agggctgacc tcgtagctcg caaccgcatc cgccgctgga atccagagct
     8881 cgcgctgctc tgcataagcg aagctcatgt acacgacact cgccgacgtc accgccgccg
     8941 caccgatcca atggcgccgc acgggactcg ggccggggcc cgcatccaac gccccccgta
     9001 aaggattggc acctttgacg ttcgtcaaac agaagtagat cccgcctcgc gcggggctga
     9061 gtgacatcac atccgtcgag tatagggccg cggctagcgt tccgagcggc tcgaccagct
     9121 ttggaagctg acgacaaaag gcaatcaccc ggcgatactg ttccgcgctg cacactccga
     9181 gagcccgcgg cagctgcaag tgcagctcgt cttcccagat gcgtagagca tcccgaaccg
     9241 gctcggcctc gagcagaggg atacgggagg acatcccgat ccgtgaccag aagtccgccg
     9301 cctccgttgc cccggtgagc cggtccaagt gagtcagcgc aatcctgagc tccacttcct
     9361 tctttcgaat cgcggagagc tggttgacgc gagcgcgcac ggagcgcgcc aagtccatca
     9421 gcgcgtcccg cgggctcgat agcagccatt gcgcatcgac cgtcaaaacg acgctcggag
     9481 ccttcttaga ataaaggggt cgccagaggc gttccagcgc ccgcttactg gtcagcgtct
     9541 catcgagcag gtatcgctcg ggtagctcca tgatcacggc gctcgaagag agcaccacgg
     9601 gcagctcggc gtcgaccggt cgctccccga tgacttgccg catttgatag tgatagtcgg
     9661 tgtgagtctc gagcaagcgg ctcttcccgc tcccggccaa gcccatcaat acgaactgct
     9721 cgaagttcaa gatcgagcgg cgatactccc ccggcaagcg ccgcgcgaag cggagccagg
     9781 cgcgccgcag agcccgactc tcgatctccg ggatgcgcgg ttcacccacg cccttcagca
     9841 cattcgggcc gagcgctcgc ttctgagcgc cgcgcttctt ccagtaatcc caggctcgtt
     9901 tgcccagcac taccgcgacg ggaaccgcga ggcccagagc gatcagcaac caagggccat
     9961 atgaacctgc ggaagagctg gcctccccgc gaagcctatc caggaccagc acccccggcc
    10021 cagccgacgt ggctggcgcc ggagccacac gcgaaggcaa accgctgccg atcggcacgg
    10081 ccaaaaccga cgcaatcgga accgggacca acgccgaggc cacgggcacg accggcagag
    10141 ccgaggcgat cgcaggggcg gccatactca ctccaccctc gagtacaggt agacgcggca
    10201 accatcgagc accggggagt cgaacatcac gactcggccc tccgacaccg cgaagtccca
    10261 ttcctgaccg taggacagcc cgtagatctc gaccgccgaa gacaggctct gcgcaaacgg
    10321 ggggcgctcg atgcggctga tcggaatgcc ggtcagagaa cgctcgtgca cggcgtgcag
    10381 ccgtgacggg ctcgagagtt tggtccggga gagctccacc ttggccgcca ccgagggctt
    10441 ttgaatcaac aggaacacgt gcttggcgcg tttgacgttc ttgtcgatgt cgcagtacaa
    10501 gaggccctct ttgcgggcga actccacgta aggcacttcg ggccggccgc gttcgagcag
    10561 caattcgatc
//
LOCUS       DASTKU010000065         8680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_458932, whole genome shotgun sequence.
ACCESSION   DASTKU010000065 DASTKU010000000
VERSION     DASTKU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458932"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFQ61_02175"
     CDS             complement(<1..797)
                     /locus_tag="VFQ61_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953278.1"
                     /translation="MRWSYFPVASALLARLLCAEAAQATPRLLELTFEAPAECGSAER
                     VEAAVGELVGTKPPRSLRASLFVRHEGSEYRAELRTGPEAIRVMRGLTCDAVVEASAI
                     VLALAIEPHAASARRAVAPSANGSTEVPADVPRSRLHPLVSAGPIFDTSTLPHVALGA
                     ELRAGISGVSWSASARGHYWLPASGSLESDAALGGRFTWWTIGISGCLGPARTRFSVC
                     LTPELGRMTAEGQGADEPQRGSATWLALALEPNLMLPLTSVLRFRASA"
     gene            933..1664
                     /locus_tag="VFQ61_02180"
     CDS             933..1664
                     /locus_tag="VFQ61_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9953279.1"
                     /translation="MSNESLPRIKLTLDASTDPEAGSSAGPPSAREASSGEASGPSAH
                     ALTRLEQQLAFIQELDQLKQVQRKSFVSGTRRLENSAEHSWHVALMAVVLAEHAAEPG
                     LDVARVVRMLLVHDVVEIDAGDTLLYAEEQAQQAQRERERAAAERLFGLLPEAQGHAL
                     RTLWEEFEARESPEAKFARAIDRLEPLLLNFMSEGAAWRRYGVVVEDVLEKNRLMAEG
                     SPALWRAAEHLVQTAVLRGYLKRQV"
     gene            complement(1677..3836)
                     /locus_tag="VFQ61_02185"
     CDS             complement(1677..3836)
                     /locus_tag="VFQ61_02185"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET9953280.1"
                     /translation="MLSSDALVDIVQAYHPSADGDLIRRAYQFAEYQHREQVRKSGEP
                     YFVHPASVASIIADLRLDTASVCAGLLHDVLEDCAVTRDTIEHEFGGEIAGIVDGVTK
                     LGKFNFTSKEDRQAESFRKMVVAMAQDLRVLLVKLCDRLDNMRTLEHMKPEAQERIAR
                     ETLDIYAPLASRLGMQRLKSELEDLSFRHLEADAYRALAGKLAKTKKERDRYTESVCR
                     TITSRLAEQGYAAEVTGRAKHMYSIHRKMKEQQCEYEQVFDTMAFRICVESVADCYAA
                     LGAMHSRWTPIPGRFKDYIALPKPNMYQSLHTTVIGPSRQRIEIQIRTHEMHRVAEHG
                     IAAHWKYKERISGGVDPKDAEKFGWLRELAEFQRTLKDPIEFLESVKIDLFPDEVYVF
                     TPKGDVQVLPRGSTPIDFAYAIHSQVGEHCSGARCNGQIVPLRYKLKNGDVIEIMTSP
                     NQEPSKDWLEFCVTTRARSRIRARLRAEQRQKSVNLGKELLENEMRDAGMSFSKFNKS
                     DAEVRRIAQHFGHSSMDELLLDIGYGKLASDKVVEAIHTNDAPDSEPPPQFRPGRIEQ
                     LVRRVTGRDGGGIKVNGIDDVLVRYARCCNPLPGDGIIGFITRGRGVTIHRRECTKAF
                     DTDPERRIDVQWDTRAKINRPVQICVTTTNKPGILAVVSQTFSAQSINISEANCRAGD
                     DGRARNVFTFHCSDISQLKSVMRALAKVSGVVEVSRI"
     gene            complement(3907..5310)
                     /gene="trmFO"
                     /locus_tag="VFQ61_02190"
     CDS             complement(3907..5310)
                     /gene="trmFO"
                     /locus_tag="VFQ61_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET9953281.1"
                     /translation="MQRVTIIGAGLAGSEAALTLASHGIPVLLYEQKPKEKTPAQSSD
                     ALCELVCSNSFRGAALSNAVGLLKEEMRRLGSFVMQAADRTRVPAGGALAVDRERFSE
                     CVTRWLHDHPLIEIRAERVDRIPAERPLVIATGPLTGEALAADIAARVGSDQLAYYDA
                     IAPIVSAESLDWSQLFFASRWEKGEDEADKAAYANSPLNAEQYRAFVEAILGATKVAP
                     KAFEDVRYFEGCLPIEVMAERGLQTLAFGPMKPVGLTDPRTGRWPHAVVQLRKEDEAG
                     TAYNLVGFQTRMTWPEQVRVFRMLPGFAEAEFLRHGAVHRNTFIQAPVLLDETMQLKT
                     EPGLYFAGQITGTEGYVESAAGGLLAALFLRERLNGRTPLLPPKTTALGGLLTHLARN
                     PESYQPSNITFSHLPPWDGGRLPKRPRYEAMAQRALTDLKNWAVDLAVPLAGSELMTS
                     SMAPVPVSEMGLSENSP"
     gene            complement(5321..6229)
                     /locus_tag="VFQ61_02195"
     CDS             complement(5321..6229)
                     /locus_tag="VFQ61_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET9953282.1"
                     /translation="MIEKRPIVLTSGSLPPLLGDLPKRPNALFLRGELPRGPAVAIVG
                     TRAPTEEGVQFAHELAAELAQAGVAILSGGARGIDTAAHRGALSVDGVTAVIAPAGFC
                     RPYPPENAALFEEIVERAGAYAALVPDDQPATRPGFFARNACLAALCHALVLVQCGLR
                     SGARNAVAHARRIGRPVFVVPSCPWIPSGRGGLQELRRGAELCEGARDVLRWLRNAGH
                     QPVSCRDGSALAASPDSSDGTGLGDTEDGSAARVLALVEGGVTHVDELSRKTGLSVGV
                     LQARLLELCLRGGLCLNPDGTYCRKH"
     gene            6355..7314
                     /locus_tag="VFQ61_02200"
     CDS             6355..7314
                     /locus_tag="VFQ61_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953283.1"
                     /translation="MRFVVATHGHCFDGLASAALFTELLQELEIRPSEIEYRACGYGS
                     GQPQANAAMLDGDDNAILDYRFAQAPRLGWYFDHHRTAFQTEQDRKVFEDQRGTHRYF
                     HDAACSSCTKLIVDVAREQFSVELSRLSELSGWADRVDSARFESAEQAVSRTDPVMQL
                     VSVVEHYGDDAFFKQTVQELRTRPLSEIANSREVKDRYRPLGKKHERFVQRVRDKGQR
                     RGRVVYVDLTETVLDTVGKFVTYALFPDSVYSVVVGLLRSGPKISVGYNPWSGRPRDT
                     DISAICARYGGGGHPVVGGISFELRDLERARTIAQQIADELSG"
     gene            7347..8099
                     /locus_tag="VFQ61_02205"
     CDS             7347..8099
                     /locus_tag="VFQ61_02205"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET9953284.1"
                     /translation="MRAARFKRDPGQPAKVLGHRGARHAAPENTLAAFELARAEGAAG
                     VELDVRIDAEGTVIVLHDPTLTRVTAGACSAHVEDLKRADWAALDVGKGEKVPLLVDV
                     LDWAKRHDLCVNVEVKADVRNRRRLLSAVVQCLAPRAAESERLLLSCFHPGIVWHLAK
                     HLPDFGVNWLVHDRQPLLGRAPAFRRLGALGVNPQHTLLSVSSVERWKAAGALLCTWT
                     VNSPELALAYDHFGVDVIISDEPGRVLSALRG"
     gene            complement(8185..>8680)
                     /locus_tag="VFQ61_02210"
     CDS             complement(8185..>8680)
                     /locus_tag="VFQ61_02210"
                     /inference="COORDINATES: protein motif:HMM:NF024761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HET9953285.1"
                     /translation="ERRRAGTAQPLKELGNDPVSGKPITVRQGRFGLYVTDGETNASL
                     RKGDSLENLNDERAHELLAERRERGDTPRQAAKKAKAAKAKAKKEAAGAAQDGAESAG
                     KPKGKAKASKAASSKAAPKAEPSKAKAKAAPKAPKTAKASAKKAAKKGSNGQTKANAQ
                     VEPE"
BASE COUNT         1566 a         2797 c         2732 g         1585 t
ORIGIN      
        1 gcgctcgcgc ggaaacgcag cacggaagtg agcggcaaca tcaggttcgg ctcgagtgcc
       61 agcgccaacc acgtcgcgga gccacgctgg ggctcgtctg caccctgccc ctccgccgtc
      121 atacgcccaa gctctggagt caaacaaaca gagaagcgcg ttcgcgcggg acccaggcac
      181 cccgaaatcc cgatcgtcca ccacgtgaag cgcccaccca gcgccgcatc cgactcgagc
      241 gagccactag cagggagcca atagtgtccc cgcgcggaag cggaccaact gaccccgctg
      301 atgcccgcgc ggagttcggc gcccaatgca acgtgcggca gcgtcgaagt atcgaagatc
      361 gggcccgcgc tcacgagcgg atggaggcga gagcggggca cgtcggccgg aacttcggtt
      421 gacccattcg cgctcggcgc aaccgcgcga cgagccgacg cagcgtgcgg ctcgatcgca
      481 agcgcgagca cgatcgccga ggcctcgacc accgcgtcac acgtgaggcc gcgcatcact
      541 cgaatggcct ccgggcccgt ccgtagctcc gcgcgatact cgctcccttc gtgacgcacg
      601 aacagagagg cgcggagaga tcgaggcggc ttggtgccca ccagttcgcc aaccgcagcc
      661 tcgacgcgct cggcggaacc acattctgcg ggcgcttcga aggtcagctc gaggagccgc
      721 ggtgttgcct gagccgcctc tgcgcatagc aagcgcgcga gcagggcgct ggcgaccggg
      781 aaataggacc atctcacgtt tgccacccgt tcgctgcgcc gcacatcacg tcttcttgcc
      841 tatgtaccat gcccgaaccc gattcatgca caaggctcgg gaggccagaa cgagagctgt
      901 cttctatctc gaacagaggt aaattggtgc cggtgtcgaa cgaatcgctg ccccgcatca
      961 agctcaccct cgacgcgtcc accgaccctg aagcaggctc gtccgcaggc ccaccgagcg
     1021 cgagagaagc aagctccgga gaagcctccg ggccatcggc gcacgctctt actagattgg
     1081 aacagcagct cgcgttcatc caggagctcg atcagctcaa gcaagtccag cgtaagagct
     1141 tcgtctcggg aacgcgacga ctcgagaata gcgcggaaca ctcctggcac gtcgccttga
     1201 tggcggtggt gctcgcggag cacgcggccg agccgggcct cgacgtcgca cgagtggtgc
     1261 gcatgctgct ggtccacgac gtggtggaga tcgacgccgg agacaccctg ctctacgccg
     1321 aagagcaagc tcagcaagcc caaagagaac gtgaacgagc cgccgcagag cgactgttcg
     1381 gactgctacc cgaggctcaa ggccacgcgc tacgcacgct ctgggaggag ttcgaggctc
     1441 gcgagagccc cgaggccaag ttcgcccgcg ccatcgatcg attggagccg ttgctcctga
     1501 acttcatgtc cgaaggggct gcttggcggc gctacggggt cgtggtcgag gatgtgctcg
     1561 agaagaaccg gctgatggcc gaaggttcac cggctttgtg gcgcgccgct gaacatctcg
     1621 tccagacggc ggtgctgcgc ggctatttga aacgtcaggt gtgagacggg caccgctcag
     1681 atgcgcgaga cctcgaccac gccgctgacc ttggccaacg cgcgcatcac gctcttgagc
     1741 tgcgaaatgt cactgcagtg gaaggtgaac acgtttcgcg cgcgcccatc gtctcccgct
     1801 cgacagtttg cttcgctgat gttgatgctc tgagcactga aggtctgact aaccacggcc
     1861 aggatgcctg gcttgttggt ggtggtgacg cagatctgaa cggggcggtt gatcttcgcg
     1921 cgtgtgtccc attggacatc gatgcggcgc tcgggatcgg tgtcgaaggc cttcgtgcat
     1981 tcgcgtcggt gaatcgtgac gccgcggccg cgggtgatga agccaatgat gccgtccccc
     2041 ggtagcgggt tgcagcaacg agcgtaacgc accagcacgt cgtcgatgcc gttgaccttg
     2101 atgccaccgc cgtcacgccc ggtgacgcga cggaccagct gctcgatgcg gcccggccga
     2161 aactgaggcg gcggctcact gtcgggcgcg tcattggtgt gaatggcctc caccacctta
     2221 tcgctggcca gcttgccgta gccaatgtcg agcagcagct catccatgct cgagtggccg
     2281 aagtgttggg cgatgcggcg gacctcggcg tcgctcttgt tgaacttcga aaagctcatg
     2341 cccgcgtcgc gcatctcgtt ctcgagcagc tccttgccca agttgacgct cttttggcgc
     2401 tgctcggccc gtagccgagc ccggatccgc gagcgggccc gcgtcgtcac gcaaaactcc
     2461 agccaatcct tgctgggttc ttggttcggc gaggtcatga tctcgatgac atcaccgttc
     2521 ttgagcttgt aacgcagggg cacgatctgg ccgttgcaac gcgcacccga gcagtgctcg
     2581 ccgacctgcg agtgaatcgc gtaggcgaag tcgatcggag tcgagccgcg cggcaggact
     2641 tgaacgtcac ccttcggggt aaacacgtac acctcgtcgg ggaacagatc gatcttcacg
     2701 ctctccagga actcgatcgg atccttgagc gtgcgctgga actccgcgag ctcccgcagc
     2761 caaccgaact tctctgcgtc tttggggtca acgccgcccg agatccgctc tttgtacttc
     2821 caatgcgccg cgataccgtg ctcggccacg cggtgcatct catgagtccg aatctgaatc
     2881 tcgatgcgct gccgagaagg gccaatcacc gtcgtgtgca gcgattggta catattgggc
     2941 ttgggcagcg cgatgtagtc tttgaaacgc cctggaatgg gcgtccagcg tgaatgcatg
     3001 gcgcccagcg ccgcgtaaca gtccgccacc gactcgacgc agatgcggaa agccatcgtg
     3061 tcgaacactt gctcgtactc gcactgttgc tccttcatct tgcgatgaat ggagtacatg
     3121 tgtttggcgc gaccggtgac ctcggccgcg tacccttgtt ccgccaaacg cgaagtgatc
     3181 gtccggcaaa cgctctccgt atagcggtct cgctccttct tcgtcttcgc gagtttgccc
     3241 gccagcgcgc gataagcgtc cgcctcgaga tgcctgaagc tcagatcctc gagctcgctc
     3301 ttcaagcgct gcatgccgag acgcgaggcg agcggcgcgt agatatccag agtttcacgg
     3361 gcgatgcgct cctgcgcctc gggcttcatg tgctccagcg tccgcatgtt gtccaagcga
     3421 tcgcagagct tgaccaggag gacccgaagg tcctgcgcca tggccacgac catcttgcgg
     3481 aagctctcgg cctgccggtc ctccttggag gtgaaattga atttaccgag cttggtgaca
     3541 ccgtccacga tcccggcaat ttcgccgccg aattcgtgtt cgatggtgtc acgggtgacg
     3601 gcacagtcct cgagcacgtc atgcagcaag cctgcacaca cgctggcggt gtcgagccgc
     3661 aagtccgcga tgatgctggc cacactcgcg ggatggacga agtagggctc cccgctcttc
     3721 ctcacctgtt cccggtgttg atactcggcg aactggtagg cgcgacgtat gagatccccg
     3781 tcggccgacg gatgataggc ctgaacaatg tctacgagcg cgtcggagga cagcacttga
     3841 taccgtgtaa accgtaacgt aacaccgcag tgtagacttc gctatggggg gtctaaatat
     3901 ccaaaatcaa gggctatttt cagaaagccc catttctgac acagggaccg gtgccataga
     3961 cgaggtcatg agttccgagc ccgcgagggg caccgccaaa tcaacggccc aattcttcaa
     4021 atcagtaagc gcacgttgag ccatagcctc gtagcgtgga cgcttgggca gacgcccgcc
     4081 atcccacggc ggcaagtgcg aaaaggtgat gttggaaggc tgataagact cgggatttcg
     4141 agccaaatgc gtgagcaatc cgcccagcgc cgtcgttttg ggcggaagta ggggcgtgcg
     4201 cccgttcagg cgctcccgaa gaaacagcgc tgcgagcaac cctccggctg cgctctccac
     4261 atacccttcg gtgcccgtga tctgccccgc gaagtaaaga cctggttcgg tcttgagctg
     4321 catcgtttcg tccagcaaaa cgggggcttg gatgaaggtg ttccggtgca cggcgccatg
     4381 ccggaggaac tcggcctcgg cgaagccggg cagcatccga aacacgcgaa cctgttccgg
     4441 ccaagtcata cgagtctgaa agccgaccag gttgtacgcg gtgcctgctt cgtcttcctt
     4501 tcggagctgc accaccgcgt gcggccagcg gcccgttcgc ggatcggtca aacctactgg
     4561 tttcatgggg ccgaaagcca acgtttgcag accacgttcg gccatcacct cgatcggcag
     4621 gcaaccctcg aagtagcgaa catcctcgaa ggcttttgga gcaaccttcg tggcgcccaa
     4681 gatagcttcc acgaaggcgc ggtattgctc cgcgttgagg gggctattcg cataagccgc
     4741 tttatcggct tcgtcctcgc ctttctccca acgcgaagca aaaaagagct gggaccaatc
     4801 cagagactcc gcgctcacga tcggagcgat cgcgtcatag tacgcgagct gatccgagcc
     4861 gacacgcgca gcgatgtccg cggccagggc ctcgcccgtc agtggcccag tagcgatcac
     4921 gagaggccgc tccgctggaa tccgatcgac gcgctccgct cttatctcga tcaggggatg
     4981 atcgtggagc cagcgagtca cgcactcgga aaagcgttca cggtcgacag ccagggcgcc
     5041 cccggccgga acccgagttc gatcggcggc ttgcatcacg aaagagccca aacggcgcat
     5101 ctcttctttg agcagcccga cggcgttgct cagcgctgcc ccacgaaagg agttcgagca
     5161 gacgagttca cacagcgcgt cggagctctg ggccggcgtc ttctccttgg gcttctgctc
     5221 gtagagcaaa actgggatgc cgtggctcgc caaggtgagc gctgcttcgg aaccggccaa
     5281 gccggctccg atgatcgtaa ctcgctgcat gaggttctct ttaatgcttg cggcaatagg
     5341 tgccgtccgg attgagacac aggccgccgc gcaaacataa ctccaagagc cgagcttgga
     5401 gcactccgac gctcagaccg gtttttcgag acagctcgtc gacgtgcgta acaccgccct
     5461 ccacgagcgc aagcactcgc gccgcgctac cgtcctcggt atcaccgagg cccgtcccat
     5521 ccgagctatc cggcgaagcc gccaacgctg agccgtcgcg gcatgaaacc ggttggtgac
     5581 cggcgtttcg aagccagcgg agtacgtccc gagcgccttc gcacagctcc gcgccacggc
     5641 gcagctcctg cagcccacca cgccctgacg ggatccacgg gcaggacggg accacgaaaa
     5701 cgggcctgcc aatccgccgt gcgtgtgcca cggcgttgcg cgcaccgctg cgcaaaccgc
     5761 attgcacgag cacgagcgcg tgacacagcg ccgccaagca cgcattgcgc gcgaagaagc
     5821 cgggccgggt cgcgggttgg tcgtccggca cgagcgccgc gtaggcaccc gcccgttcca
     5881 ctatttcttc gaacaaagcc gcgttttccg gcgggtaggg ccggcaaaaa cccgcgggag
     5941 cgatcacggc ggtaacaccg tcgaccgata gcgcgcctcg atgcgcagcc gtgtcgatgc
     6001 cgcgggcacc gccagagagg atcgccaccc cggcttgcgc gagctccgcc gcgagctcgt
     6061 gcgcaaactg cacgccttct tccgtgggag cgcgcgtgcc gacgatggcg acggcggggc
     6121 cgcgcggcaa ttctccccgt aagaacagcg cgttggggcg cttgggtaaa tctccgagca
     6181 gagggggaag gcttcccgat gtcagaacga tgggacgctt ctcgatcact ccctcttctc
     6241 ggctcgttcg cgggaggagt tgcgcaacac gctcacgatc ccaagaatgg gccactcagc
     6301 cgaaaaccgc ggtccttcac gcgggagcgc gtgccctgtg gtaacagact ctgcatgcgt
     6361 ttcgtggtcg caacccacgg ccactgcttc gacgggctgg caagcgccgc gctcttcacg
     6421 gagctgttgc aagagctcga gattcgtccc tcggaaatcg agtatcgcgc ttgcggatac
     6481 ggttccggtc agccgcaggc caatgccgcc atgttggacg gcgatgacaa cgccatcctg
     6541 gattatcgct tcgcgcaagc gccacgactg ggctggtact tcgaccatca ccgaacggct
     6601 tttcaaacgg aacaggaccg caaggtcttc gaggaccagc gcgggaccca ccgctacttc
     6661 catgacgcgg cgtgttcgtc ctgcaccaag ctgatcgtcg acgtcgcgcg cgagcaattc
     6721 tcggtcgagt tgagccggtt gtcggagttg agcggctggg cggatcgcgt cgattcggcg
     6781 cgcttcgaga gcgcggagca ggccgtcagc cgcaccgatc ccgtgatgca gctggtcagc
     6841 gtagtggagc attatggcga cgacgccttc ttcaagcaga ccgtgcagga gctgcgcacc
     6901 cggcccttgt ccgagatcgc aaattcccga gaggtcaagg atcgctatcg tccgctcggc
     6961 aagaagcacg agcgcttcgt gcagcgagtg cgggacaagg ggcagcgccg cgggcgcgtg
     7021 gtttacgtcg atctcaccga gaccgtgttg gatacggtcg gcaagttcgt gacctacgcg
     7081 ctgttcccgg actcggtcta ttcagtggtg gtgggcctgc tgcgcagcgg accgaaaatt
     7141 tcggtaggct acaacccgtg gtcagggcgg ccccgagaca cggatatcag tgccatctgc
     7201 gcgcgctacg gcggcggcgg gcaccccgta gtcggcggga tctcgttcga gctacgcgat
     7261 ttggagcgag cgcgcaccat cgctcaacaa atcgccgacg agttgtccgg ctgatcgccg
     7321 gccgttcggg aaagcccaaa gcattcatgc gcgccgctcg tttcaagcga gatccgggtc
     7381 aacccgctaa ggtcctgggg caccgcggcg ctcgccacgc cgcaccggag aatacgctgg
     7441 cggcgttcga gctcgcacga gcggagggcg ccgcgggcgt ggagctagac gtgcgcatcg
     7501 acgccgaagg aacggtgatc gtgctgcacg atccgacgct gacccgtgtg acggctggag
     7561 cttgctcggc gcacgtcgaa gatctgaagc gcgccgactg ggcggcgctc gacgtgggca
     7621 aaggcgagaa ggtgccgctg ctggtcgacg tgctcgattg ggctaagcgc cacgacttgt
     7681 gcgtcaacgt cgaggtcaaa gcggacgtcc gcaatcggcg ccgactgctc tcggcggtcg
     7741 tgcaatgttt ggcgccgcgc gccgcggaga gtgagcggct tttactctcc tgttttcacc
     7801 cgggcatcgt ctggcacctc gcaaagcacc tgcctgactt cggcgtcaat tggctcgtcc
     7861 acgatcgcca acccttactc gggcgagcac cagcctttcg ccgcttgggc gcgctgggag
     7921 tgaatccgca acacacgctg ctatcggtgt cgagcgtcga gcgctggaaa gcagccggtg
     7981 cgttgctctg cacgtggacg gtgaactcgc cggaactcgc gctggcttac gatcatttcg
     8041 gcgtcgacgt gatcatcagc gatgaacccg ggcgcgttct gagcgccctt cgcggttgat
     8101 cgagcatgcg tcgggccgct ccagccttgg gctcgagttg cccgacgcag gtcggcgccg
     8161 cgaaatccgg acgccagctc gtagttattc cggctcgacc tgagcgttgg ctttggtctg
     8221 accgttactg cccttcttgg cggccttctt cgcggaagct ttggcggtct tgggagcctt
     8281 cggcgctgcc ttggccttgg ctttgctcgg ctctgctttt ggagcggcct tgctcgaagc
     8341 ggctttgctg gccttggctt tgccctttgg cttccccgcg gattcagcgc cgtcctgagc
     8401 ggcgcccgcg gcttctttct ttgccttggc tttggcggct ttggccttct tcgcagcctg
     8461 acgcggcgtg tccccacgtt cgcgccgctc ggcgagcagc tcgtgggcgc gttcgtcgtt
     8521 caggttctcg agcgagtccc ccttgcgcaa agaagcgttg gtctctccgt ccgtgacgta
     8581 cagtccaaag cggccctgtc gaacggtgat gggcttgccg ctcaccggat cgttgccgag
     8641 ctccttcaag ggctgcgcgg tgcccgcgcg tcgccgctcc
//
LOCUS       DASTKU010000066         6913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_458990, whole genome shotgun sequence.
ACCESSION   DASTKU010000066 DASTKU010000000
VERSION     DASTKU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458990"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="VFQ61_02215"
     CDS             complement(<1..1444)
                     /locus_tag="VFQ61_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9953286.1"
                     /translation="MKFFRKSSPATVSEPTPPRNSLLTMAPAAVAVAAPDLEQRRRWI
                     RMRMGMLCGLLAIGLGLVVSAGYTIMVDDGAAWRELAESQRQRRLHVAPKRGTIYDRG
                     GGALAVSVEVPSVSLDAVELLRGVPPQRIPVVARDAANRVAGVLGLDPAQVERRILAK
                     RRFAWLKRQISAEEADKIRELGQQGVRGLNVEGEGRRYYPRRELAGPLLGFVAPDGEG
                     KDGLEFAQNNELRGQVEQLRGLRDRSGRLVFSEGIADEQALAGHNLVLSIDQGVQFVA
                     ERELALAARTFEAKGGSVVVVEPHTGEILAMASFPQFNPNDYRESEPEARRDRALTEA
                     FEPGSTAKIFTVSAGLAANVLRPTDKLYCEKGTMAVDNVVIRDTHPSEWLTLAQVLAV
                     SSNICAAKIGLSLGADRLYEALRRFGFGEPTGLPFPGEASGTLRPRGRAWVQVETAAA
                     SFGQGVSVTNLQLAMAAAAIANGGELMEPIL"
     gene            complement(1441..1785)
                     /locus_tag="VFQ61_02220"
     CDS             complement(1441..1785)
                     /locus_tag="VFQ61_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953287.1"
                     /translation="MREERTFLALWVLAVAAATAAFVLHLGLRVRSVELGYELGRVHA
                     RVQRLREVKHVLEVELFSHKTPERVDFVARTLLGMSEPTPDRIRNAGPKPRADKLENA
                     VTRATDEEETGP"
     gene            complement(1782..2789)
                     /gene="rsmH"
                     /locus_tag="VFQ61_02225"
     CDS             complement(1782..2789)
                     /gene="rsmH"
                     /locus_tag="VFQ61_02225"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET9953288.1"
                     /translation="MPVTPLDDTPGFLHVSVMLEEVISAFSPMLTGGVLMGGGLMGGV
                     LPGSSSAGEGSAPPMSIIIDATAGGGGHSEALLERYPNVRLLAFDRDTSAVQAASARL
                     ARFGVRARVTHSAFSEIGSWLDAFGIEKVDGLMADLGVSSPQLTAPERGMSFRAEGPL
                     DMRMDPSRGETALELMERVSQDELANIIYELGEERGSRRIARCIKQALLQGELHTTLD
                     LRRAVVRAMGPRRIGGVDPATRTFQALRIAVNRELDELASLLSLAHERLAPGGTAAFI
                     SFHSLEDRLVKRAFAERTEYQRVTPKPLIPTEAEQNANARSRSAKLRVARRLPASLES
                     K"
     gene            complement(2792..3238)
                     /gene="mraZ"
                     /locus_tag="VFQ61_02230"
     CDS             complement(2792..3238)
                     /gene="mraZ"
                     /locus_tag="VFQ61_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET9953289.1"
                     /translation="MFRGQFQHSVDAKGRISVPARFRELLTADGTTRFIVTPAAFDPC
                     LHVYPMRAWEEFERKISDLPSLDANVTRFRRIYVSAAVECELDAAGRLLVPPALRERA
                     RLEKDAIWAGMGRVIELWSKPEFDRSLTMTADEAAAFRAAMEQMKL"
     gene            complement(3700..4422)
                     /locus_tag="VFQ61_02235"
     CDS             complement(3700..4422)
                     /locus_tag="VFQ61_02235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HET9953290.1"
                     /translation="MSRFVRVPHVFAGPGSSELPRETAHYLTHVHRARVGEEFVVFDV
                     EAGLEASARVVGLERGRVHCELSEPRPVSPSPLRVGILQATAKGDRVEQVVRAATALG
                     ARWVKTVICERSVARPAELRLGRLQAIALDAARQSGRGDVPLLSGPTPLAQALEEPWS
                     GLSFCAALGSEATLAHHVQAWDLESDVRILIGPEGGLSEAELAGAERAGFVRVSLGPF
                     TLRTELAATAALANFAARLGCV"
     gene            4678..5397
                     /locus_tag="VFQ61_02240"
     CDS             4678..5397
                     /locus_tag="VFQ61_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953291.1"
                     /translation="MSLNHCRSLSLSVLGLCLAVVGCGGEEVKYTPRPAYSGSKANLP
                     PVPNLPKKPIKNGESYTIWGASYSLRSRVHKKEVANKKITLTGYINKTNLAEAPECAV
                     HKGGKADPENCKPPVPAFWICDSKDAPITECIKVMGWASNFAQVWEAIHEFDTGRPKK
                     DQTEVEYTDTFWGVKVPNPLPAVGAKVSVSGNYGVSFTQSSTGAEADPVMGLMSFQEM
                     KELEPAPELATLPGVKRKPKK"
     gene            5483..>6913
                     /locus_tag="VFQ61_02245"
     CDS             5483..>6913
                     /locus_tag="VFQ61_02245"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HET9953292.1"
                     /translation="MSARHPGNYVSTASAAAIRNASLAIAFLIAFAAGCRPLPRDPIV
                     ESLQIRGVRSVDVDAIEQGLSTGESPKLFGFIPRVLEYSTYDAGILERDLLRIERYLR
                     ARGYYSAKVIAARVIHTSTHSVRVQISVQEGEPVRVSRIEPGISALPFDVMLIANRAR
                     EMTEGDLLDEDRFEADRARIERALFDAGYAFAQVKVQAVVDLPSHTATLRYDLQLGPK
                     AVLGKVMIVGLQEIPEGPVRDNLNLSEGDTFSESDLEDAQQALLNLGVFSNVDVLADR
                     THPETGRVPITVQLRETALRSVRAGVGARFDVQRFASVFEVGWEHKNFFGGMRRFSIQ
                     ARPGLTFYPTRIDRLELWSRYLPENRLGVTLRQPSFLEGRTTLAIEGEYNVYPLLYPL
                     PESADARTERIIGYQELRSAVRLERAFLNHHLNVAPSLNFQANFPFAYQAGGPGLNAV
                     RVSFPELVTSLTFVDNVLEPHSGVSLS"
BASE COUNT         1267 a         2341 c         2156 g         1149 t
ORIGIN      
        1 ccaggatcgg ctccatcagc tccccaccgt tcgcgattgc ggccgcggcc atggccagct
       61 gcaagttggt tacgctcacc ccttgcccga acgaagctgc cgcggtctcg acctggaccc
      121 acgcccgccc gcggggccgc agagtgcccg aagcttcacc cgggaacggc aagcccgtgg
      181 gttcgccgaa gccgaagcgg cgcagggctt cgtacaagcg atcggcgccc agcgacaggc
      241 cgatcttcgc cgcacagata ttcgatgaaa ccgcaagaac ttgggccagc gtcaaccact
      301 ccgaagggtg agtatcgcgg atcaccacat tgtccaccgc catcgtgccc ttctcgcagt
      361 agagcttgtc ggtaggacgc agcacgttgg ccgccagccc cgcactcacg gtgaagatct
      421 tggcggtgga gcccggctcg aacgcctcgg tcagcgcccg atcacgtcgc gcctcgggct
      481 cgctctcgcg atagtcgttc ggattgaact ggggaaagct cgccatcgcg aggatttccc
      541 cggtgtgcgg ctcgaccacc accaccgagc cgcctttggc ttcgaaagtt cgcgccgcca
      601 aggccagctc acgctccgcc acgaactgca cgccctgatc gatcgacagc acgagattgt
      661 gcccggccag cgcttgttcg tccgcaattc cctcggagaa caccaggcgc ccagagcgat
      721 cgcgcaggcc gcgcagttgc tcgacctggc cgcgcagctc gttgttctgc gcgaactcta
      781 gcccgtcctt gccctctcca tccggggcca cgaaaccgag cagcggtccc gcgagttcgc
      841 ggcgcggata gtagcggcgc ccctcgcctt ccacgttgag gcctcgcacc ccttgttgtc
      901 cgagttcgcg gatcttgtcg gcttcttccg cgctgatctg ccgcttcaac caagcgaacc
      961 gacgcttcgc caggatccgt cgctcgactt gggccggatc gagacccaag acccccgcca
     1021 cccgattggc cgcatcgcga gcaacgaccg ggatcctctg cggcggtacg ccacgcaaca
     1081 gctcgaccgc gtccaggctg acgctgggca cttccacgct gaccgcgagc gccccaccgc
     1141 cgcggtcgta aatggtgcct cgcttgggag cgacgtggag gcggcgctgg cgctggctct
     1201 ctgccaactc gcgccaagca gcgccgtcgt ccaccatgat cgtatagccc gcagacacga
     1261 ccagaccgag accgatcgcg agcaagccgc agagcatgcc catacgcatc cgaatccaac
     1321 ggcgccgctg ctcgagatcg ggagccgcaa ccgcgacggc cgcgggcgcc atcgtcaaca
     1381 gtgaattgcg cggcggcgtc ggctcggaga ccgtcgcggg cgaggactta cggaagaact
     1441 tcatggtccc gtctcctcct cgtcggtcgc tcgggtcacg gcgttctcga gtttgtccgc
     1501 gcgcggtttg ggccccgcat tgcggattcg gtcgggtgtc ggctcactca tgccgagcaa
     1561 tgtccgggct acgaagtcta cacgctccgg ggtcttgtga ctgaacagct cgacctccaa
     1621 cacgtgcttg acctcacgca ggcgttgcac ccgcgcgtgc acacggccga gctcgtagcc
     1681 caactccacg ctgcgcacgc gcagccccaa gtgcagcacg aacgcggcgg ttgccgcggc
     1741 cacggccagc acccaaagag ccaggaacgt gcgctcctct ctcatttgga ctccaacgag
     1801 gcgggcaagc gtcgagccac gcgcagcttg gcgctgcgtg aacgtgcgtt ggcgttctgc
     1861 tccgcctcgg tgggtatcaa cggcttgggc gtgacgcgct gatactcggt gcgttccgcg
     1921 aaggcgcgct tgaccaaccg gtcttccaac gagtggaagc tgatgaaggc ggcggtgccc
     1981 ccgggcgcca gccgctcgtg ggccaggctg agcaggctcg cgagctcatc gagctcacgg
     2041 ttgaccgcga tgcgcagcgc ctggaatgtc cgggttgccg gatccacgcc gccgatgcgc
     2101 cgcggtccca tcgctctgac gacggcgcgc cgcagatcga gtgtggtgtg cagctcacct
     2161 tgcagcaagg cctgcttgat acaacgtgcg atgcggcgcg agccgcgctc ttcaccgagc
     2221 tcgtagatga tgttcgcgag ttcgtcttgt gagacgcgct ccatcaactc gagcgcagtc
     2281 tcgccgcggc tcgggtccat gcgcatgtcg agcggccctt cggcgcgaaa ggacatgccg
     2341 cgctcgggcg cggtcagctg cggggagctc acgccgagat ccgccatcaa gccgtccacc
     2401 ttctcgattc cgaacgcgtc cagccaagag ccaatctcgg aaaacgcgga gtgagtgacg
     2461 cgagctcgaa ccccgaagcg agccaagcgc gcagaagccg cttgcacggc cgaggtgtcc
     2521 cgatcgaaag ccagcaagcg cacgttcgga taacgttcga gcagcgcctc cgagtggccg
     2581 cctccgccgg ccgtagcgtc gatgatgatc gacatcggag gtgcgctccc ttcgcccgcg
     2641 ctggagctgc cggggagcac gccgcccatc agccccccgc ccatgagcac gccgccggtg
     2701 agcatcggcg aaaaagcgga gatgacctcc tccagcatca cggagacgtg gagaaagccc
     2761 ggtgtgtcat cgagaggagt taccggcatc gtcatagctt catttgctcc atggcggctc
     2821 ggaaggccgc cgcctcgtcg gccgtcatgg tgagcgagcg atcgaactcg ggcttcgacc
     2881 acaattcgat aacgcgaccc atgcccgccc agatcgcgtc cttctccaag cgcgcgcgct
     2941 cgcgcaacgc gggcggcacc aacaagcggc ccgcggcatc gagctcgcac tcgacggctg
     3001 cagacacgta gatccggcga aaacgtgtga cgttcgcgtc gaggctcggc aggtccgaga
     3061 tcttgcgctc gaactcctcc caagcccgca tcgggtaaac gtgcagacac ggatcgaacg
     3121 cggccggagt cacgatgaag cgcgtggtcc cgtccgccgt cagcaactcg cggaagcgag
     3181 cgggcacact gatgcgccct ttcgcgtcga cggagtgctg gaactggcca cggaacatcg
     3241 gttcgctcgt cccttgggaa gccgcgacac tttctcccac ggcttcccac tggactcggc
     3301 gaacctacgg gtcgcagggc cgttctgtca acaatgcggc ctgccgaaat cttgagaatt
     3361 ctattcaact cccgtaatgg gagtatctca cctgctctga cgttcagcgt gggaataagt
     3421 ggggcgcttc ccaggcgtga cgcgcaatac agcccagagc agagccaccg acacgaacgc
     3481 gggcaagcca acgagcagga gcatgcgcct cgtactgcaa gcgtcgggcg gaaggtcgtg
     3541 ctctccgaaa cagcgtaatt cgtgctggat tggcgttgtc gactcagaac cgcgtggctc
     3601 tgaagacacg agccctgtgg ataaaccccc actcactccg cgagagccgc gtcctcgcgc
     3661 agtctcagcc tgacacgcgc gagaagagca gccgcgccgc tacacgcacc cgagccgcgc
     3721 cgcgaagttc gccagcgcgg ccgtcgccgc gagctccgtg cggagcgtaa acgggcctag
     3781 cgacacgcgc acgaagccgg cgcgctctgc gccggcgagc tctgcttcgc tcaagccacc
     3841 ttcggggccg atcagaatcc gcacgtcaga ctccaaatcc cacgcttgga cgtgatgcgc
     3901 caacgtagct tcggacccca gagcggcgca gaaagacaag ccggaccaag gctcttcgag
     3961 cgcctgggcg agcggcgtag gtccggagag caagggcacg tcgccgcgcc cgctctggcg
     4021 cgcggcatcg agtgcgatgg cttgcaggcg gccgagacgc agctccgcgg ggcgcgccac
     4081 gctccgctcg caaatcacgg tcttcaccca acgcgcgccc agggcggtcg ccgcgcgcac
     4141 cacctgctcg acgcgatccc ctttcgccgt cgcctgcaga atccccaccc gcagcgggct
     4201 cggagagacc ggtctcggct ccgagagctc gcaatgtacg cgacctcgct ccaggcccac
     4261 gactcgcgcc gacgcttcca gccccgcctc gacgtcgaag accacgaact cttctcccac
     4321 ccgagcccgg tgcacatgag tcaggtaatg ggccgtctcc cgcgggagct cgctcgaacc
     4381 agggccggca aaaacgtgtg ggacgcgcac gaaacgagac acgcgcgagg gttgccacga
     4441 aatccagcgt gcagccgcta gcaagcgcta cctgctgcag atcgcggatt cccacccctc
     4501 aaaaaaattc cccagtcgaa cgcttgcggg caccagcctc aactccgcgg cgaagcgact
     4561 gccgggccgt ttcgggcgcc ggcccgggcg gtgttcgtcg tgtaacgcga aatccgtgtt
     4621 gaagtgaggg aagaacgcag actactgtcc cccgtgtttc cgaggagacg taaagccatg
     4681 tcgctgaatc attgccggtc gctctctctc tccgttctcg gcctttgtct tgctgtcgtg
     4741 ggctgcggcg gggaagaggt gaagtacacg cctcgaccgg cttactccgg gagcaaggcc
     4801 aatttgccgc ccgtgccgaa cctgcccaag aagccgatca agaacggcga atcctatacg
     4861 atctggggcg cgagctattc gctccgcagc cgcgttcaca agaaggaagt cgccaacaag
     4921 aagatcacac tcacgggcta catcaacaag accaacctgg ctgaggctcc cgagtgtgcg
     4981 gtgcacaagg gtggcaaagc cgatcccgag aactgcaagc cgcccgtgcc cgcgttctgg
     5041 atatgcgaca gcaaagatgc gccgatcacc gagtgcatca aggtgatggg ctgggcctcg
     5101 aacttcgcgc aagtttggga agccattcac gagttcgata ccggccgacc caagaaggac
     5161 caaaccgagg tcgagtacac ggacaccttc tggggcgtca aagtgccgaa tccgctgccg
     5221 gccgtcggcg ccaaggtgtc cgtatcgggc aactacggcg tttcgttcac gcagtcctcg
     5281 accggcgccg aagcagaccc ggtaatgggt ttgatgagct tccaagagat gaaagagctg
     5341 gagcccgcgc ccgaactcgc gactttgccg ggcgtcaagc gcaagccgaa gaagtgaggg
     5401 cgtaaagacg ccaccgagcc acgcgctgcg gcgcgtggtc tctgcttggc attctttcac
     5461 cgacggccta cgaaggtgcc gcttgagcgc gcggcacccc ggtaactacg tttccacggc
     5521 ttcggccgca gcaatcagga acgcatctct cgcgatcgcg ttcctgattg catttgcagc
     5581 gggctgccgt ccacttccgc gcgatccgat cgtggagtcg ctgcagatcc gcggtgtgcg
     5641 ctcggtggac gtagatgcca tcgaacaagg gctctcgacc ggcgagagtc ccaagttgtt
     5701 cggcttcatc ccgcgcgtcc tcgagtactc cacgtacgac gccggcattc tcgagcgcga
     5761 cctgctgcgc atcgagcgct acctgcgggc gcgcggttac tattcggcca aggtgatcgc
     5821 cgcgcgggtg atccacacgt cgacgcacag cgttcgcgtg caaatcagcg tccaagaagg
     5881 cgaaccggtg cgggtcagtc gtatcgagcc tggcatctcg gcgttgccat tcgatgtgat
     5941 gctgatcgca aatcgtgcgc gagagatgac ggagggggat ctgctcgacg aagatcgatt
     6001 cgaggccgat cgagctcgca tcgagcgggc gctgttcgat gccggctacg ccttcgccca
     6061 ggtcaaggtt caggccgtcg tggacctgcc gtcgcacacc gcaacgctgc gctacgacct
     6121 tcaactcggg cccaaggcgg tgctcgggaa ggtgatgatc gtcgggctcc aggagatccc
     6181 cgaagggccc gtgcgcgaca atctgaattt gagcgaagga gacaccttca gcgaatccga
     6241 tttggaagac gcgcagcaag cgctgctcaa cctgggcgtc ttttctaacg tcgacgtgct
     6301 cgcggatcgt actcaccccg aaaccggtcg ggtgccgatc acggtgcagc tccgggaaac
     6361 ggcgttgcgc agcgtgcgcg cgggcgtggg tgcgcggttc gacgtgcaac gcttcgcgtc
     6421 ggtcttcgag gtgggttggg aacacaagaa cttcttcggc gggatgcgcc gcttcagcat
     6481 ccaagctcgg ccgggcctga ccttctaccc gacgcggatc gatcggctcg agctttggag
     6541 ccgataccta ccggagaatc ggctgggcgt gacgctgcgt cagccctcgt tcctggaagg
     6601 tcgtacgacc ctggccatcg aaggcgaata caacgtttat cccttgctct atccgctgcc
     6661 agagagcgcg gacgcgcgca ccgagcgcat catcggttac caggagcttc gcagcgcggt
     6721 tcggctcgag cgcgcgttct tgaatcacca cctgaacgtg gctccatcgc tgaattttca
     6781 ggccaacttc ccgttcgcgt atcaagcggg aggcccaggg ctcaacgcgg tgcgtgtgtc
     6841 tttcccggag ctcgtgacgt cactgacgtt cgtggacaac gtgctcgagc cacactccgg
     6901 ggtttcgctt tcg
//
LOCUS       DASTKU010000067        10515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1573787, whole genome shotgun sequence.
ACCESSION   DASTKU010000067 DASTKU010000000
VERSION     DASTKU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10515
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573787"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3532
                     /locus_tag="VFQ61_02250"
     CDS             <1..3532
                     /locus_tag="VFQ61_02250"
                     /inference="COORDINATES: protein motif:HMM:NF027309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HET9953293.1"
                     /translation="GRSATLQLCALPVLLFALSACGDDDDDVPSGTGGSGGSTAGATS
                     GGRGGSGGSGGATTGTTTGGRSTAVTGGSGGKTTVPPEGGAAGETPVTGGAAGQTGET
                     GGTTSIPPGGAGGVGGEGGEGGASGGTTGMWGEPPKNGVDPWDSKPTRGVVKDPGQYV
                     SRDAGAGRFPLATAGKAAPIVVSKADFWGVQRTAKDFQADVKRVTDIEPAYSEDTIPA
                     GAKNVILVGTLGKSPLIDKLVQDKKLDVSDIAGLWETFLIQVVENPMAGVDRALVITG
                     SDQRGTSFGIYDVSKQIGVSPWYFWDDVPARKSNSLYVLPGHHTRGEPAVKYRGFFIN
                     DENPQLSTWAHNTFGDSPNKDFPMAFHHPLYEKVFELMLRIKANYLWPAVWGRAFAED
                     DPMNHEMAKAYGVVMGTSHEAPMMRGIEEWNRHAVGQTTDANGNITNPGSDPYGGNGL
                     WRFSKNPDALKLYWADGIKRMVDQEFEGVVTLGMRGPGDVSLPVEDGIELMNSVIAAQ
                     RGILEDETGKPAWAASPQVWTLYKEVQDYWDKGMRVPEDVTVVWCDDNWGNMRELPVQ
                     SEAKRSGGYGLYYHFDYVGGGRNYKWVDTNLLPNIWEQLNLSYSYGVNRLWVVNVGDM
                     KNEEHPLEFFMDYAWSPEQWPLERLADWQEQWAAQQFGADYAVAIKDILHDYSRLQSL
                     RKPELTNRSITLNPAKDISDPAQTGDAVVYDDAASPFSLLNYRELETLAEDWKNLAQD
                     AAVVRDSLPAELKDAYYQLVFYQVQATAYLYEHRLAVFKNKLYQAQGRASTNDWGTKA
                     QAAFDEGRKLAAYYNDTLAGGKWKGWQTQPYIGYGDVERYGSNAGWQQPELNNAAIED
                     APYPHIVTNFTPVTGRSIGVSVEGSDKVWPIETATTAQLPKISRYQTQPNPYIEIFNR
                     GDLFTEYTLNIPQNAQSWLSVTNAAGRVGNTNKEVRAEVQVSNWANVPAGTTQVTLTL
                     TGTGVGTPIPVNVVVENTSVPADFRGFVEGGGYVAMLAEHYSRKVDSGAVGFRLLPDI
                     GRLSSGLTPFPVNAPPQTPGDAAAPHLEYDVYLTSAGETTLTSYLSPRNNVLHSDGLK
                     YAVSIDNGTPEIVNITKALNGIPMNRSWERNTSDNVNRTTTKHTVTAGKHTIKFWMVD
                     PTIIVQKLVLDTGGARPSYLGPPESFRKTN"
     gene            3988..7350
                     /locus_tag="VFQ61_02255"
     CDS             3988..7350
                     /locus_tag="VFQ61_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HET9953294.1"
                     /translation="MTRNLTALHWGALPVVLFVLSACGEEGSSGWAAGAPSLGGARSS
                     GGAGGSNGGATSSGGTSSTPGGTTSVAGSTAVQPGVDPWDSKPDRGVVKDPGRYVATE
                     DGAGRFPLVSNRKAVPIVMSGKDFWGVQRTAGDLKADIERVTDVEPELVSDTPPSGAK
                     DIVLVGTLGKSPLIDQLVKDKKLDVSDIEGRWETFLIQVVQKPMANVDRALVITGSDQ
                     RGTSFGIYDLSKQIGVSPWYYWDDVPARKSSALYVLPGHHTMGEPAVKYRGLFINDEN
                     PQLGTWAYNTFGASPNASFPQAFHKKMYEKVFEVMLRIKANYLWPAVWGRAFAEDDPE
                     NHATAKAYGIVMGTSHEAPMMRGIEEWNRHAVPEVSDASGVVTTQGMDPYGGNGLWRF
                     SKNPNALKGYWTDGIKRMVKEDFEGVITLGMRGPGDVSLPVEDGIGLMNNVIKEQRAI
                     IAAETGKDVTALPQVWTLYKEVQDYWDKGMRVPDDVTVVWCDDNWGNMRELPLQTEPK
                     RWGGYGLYYHFDYVGGGRNYKWVDTNLLPNVWEQLNLSYSYGVNRLWMVNVGDMKNEE
                     HPLEFFMDYAWSPERWPLESLPQWERQWAAQQFGEEYSVAIADVLHAYSKLQSRRKPE
                     LTNRSITLDPAKNISDPGQTADAVKYDDKASPFSLTNYRELERMVEDWNQLASEAVTI
                     GESLPKEARDAYFQLVLYQVQASAELYNHRLAVFKGLLYKAQGRWSTNDQGAKAQEFF
                     DKGTALAKMYNETIAGGKWKGWQTQPYIGYGDTERYGSNASWQQPEKDNAALPDAPYP
                     QIVTDFMVTDGKDVGVTVDGSDQVWTAKTPAETPPASLPTFSRYQVQPTQYIEVFRTG
                     SGTDEVPFSITAPAEATWLTITPSTGTLNATQKDARIEVKVDWTKAPAEAQTVKLTVA
                     TGASSSRTTVTTVNAVIDPKLVPETFSGFVEANGYVSLEADHFSRIIDGPSVGFRKLP
                     EIGRTGSGMTPFPVTAAAQKPGGTSPHLEYDVYLGTGGDITVWTFCSPRNNVLHGDGL
                     KYAISIDDAEPQIVNITTALNGIPMNKSWERNTSDNVNRTSVALKGVTPGAHVLKLWM
                     VDPTVVVQKLVVDTGGLKPSYLGPLESHRITR"
     gene            complement(7572..9032)
                     /locus_tag="VFQ61_02260"
     CDS             complement(7572..9032)
                     /locus_tag="VFQ61_02260"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9953295.1"
                     /translation="MQSDHRDDDTGSVDQKPSDRFAPDRFATGTLLGGKLRIIRRLGF
                     GGMGAVYEVEHELTHHRRALKLLHAHAARIPTVVERFLREASAAGRIGNAHIVETFDA
                     GRLDSGEPYIVMELLQGKTLSEVLIEQGPLPIDATCDILIQACDAVSAAHAVGIVHRD
                     LKPENLFLTGPALSFVKILDFGVSKFDVATTGEHGLTLEGSPIGTPYYMSPEQVRGER
                     SLDGRADIYALGVLLYECLTGRKPFVADTLPHLAVLIHQGKYPTPSEVRQGLPAAVDL
                     VVSRAMASERADRYESAAEFSRAIARLRDSISPIALSPTQLGNPGSLASASTPLHDAS
                     SRAPAGGRVTRPWFIVAAVGALLVVSGLTIALRERSASDRTQGLTSDAKNPIPDASAL
                     ASASSSTSPPSKLSPSSAPEAPARTEEIDATSAIGNSSVEPAASVIASARPGPARPHS
                     GPASSSNRVPAAGSPGTSIPRSRASSYGLSQDNPFK"
     gene            9109..>10515
                     /locus_tag="VFQ61_02265"
     CDS             9109..>10515
                     /locus_tag="VFQ61_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9953296.1"
                     /translation="MRARLSGLRIPVVLCSIASLCAPALTLAQPAPSAEGAQQSPEAA
                     QQRRAEAAERFDRALKLFEEGNNASALAEFRRVQEISPNPIVLYNIGLVSAAMGRAVD
                     AVDALTEVLKNPEALGPARAARARVALTEQSTRIALLDVSVNTEVARIEVDGVEAGRA
                     PLSAPLRVTSGSHLVGAVAEGYVPERREVLVAGGAITPLRFELVEMRGRRLANLSLRT
                     NVLAAEVVIDGAVVARTPLAATLALAPGQHRIELRRAGYQPRAQTLELGDGATGELTW
                     PLEIDPAALTSQGADVTLEISEPGAQLSVDGVSSGLYAGPLRLPVGPHHVRVERAGFL
                     SWERVIQVDPHKPNGVNVELEPTPQTRAEVARNAHQHRLWGIVGVAGGAVIAGLGTGW
                     LLHNGARKSDARQAVDAINAEASSDSGRCDTSSGAVTQEQCNASVDAAYSEWNSVKKR
                     DVYGWIGVGVGAAALTTGI"
BASE COUNT         2088 a         3181 c         3432 g         1814 t
ORIGIN      
        1 gggccgctcg gcaacgctgc agctctgtgc gttgcccgtc ttgttgttcg ccctctcggc
       61 ctgtggagat gacgacgacg acgttccgtc aggaacgggc gggtccggcg gtagcaccgc
      121 cggcgcgacc agcggaggcc gcggtggatc gggtggaagt ggtggagcga ccaccgggac
      181 cacgacaggt ggccgaagca ccgccgtcac cggtggctcc ggcggcaaga caactgtgcc
      241 acccgaaggt ggcgctgcgg gtgaaacccc agtcacgggg ggggccgcag gccaaacggg
      301 tgaaacgggt gggaccacta gcattcctcc gggaggtgcc ggcggagtgg gtggcgaagg
      361 cggagaaggt ggagcctctg gaggcaccac gggcatgtgg ggtgagccac caaagaatgg
      421 cgttgatccc tgggattcca agccgacccg cggagtggtg aaagatccgg gtcagtacgt
      481 ttcgcgtgac gcgggtgcgg gtcgattccc ccttgcaacg gccgggaagg ccgcgcccat
      541 cgtggtcagc aaagcggact tctggggcgt gcagcgcaca gccaaggatt tccaagctga
      601 cgtcaagcgc gtcacggaca tcgaaccggc gtattccgag gacacgatcc ctgcaggcgc
      661 caagaacgtc attctcgtag gcactttggg taagagcccg ctcattgaca agctcgtcca
      721 ggacaagaag ctggacgtca gcgacatcgc cgggctgtgg gagaccttcc tgatccaggt
      781 cgtcgaaaac ccgatggcgg gcgtggaccg ggcgctcgtc atcaccggca gcgatcagcg
      841 cggcacgagc ttcggtatct atgacgtgtc gaaacaaatc ggcgtgtcgc cttggtattt
      901 ttgggatgac gtgccagcgc gcaagagcaa ctcgctctac gtgctcccgg gccatcacac
      961 gcgcggtgaa cctgcggtca agtaccgcgg cttcttcatc aacgacgaga accctcagct
     1021 ctcgacgtgg gcccacaaca cgttcgggga ttcgccaaat aaggattttc cgatggcgtt
     1081 ccaccacccg ctatacgaga aggtgttcga gctgatgctc cgcatcaagg cgaactacct
     1141 gtggccggcg gtgtggggtc gtgccttcgc cgaggacgat ccgatgaatc acgaaatggc
     1201 caaggcctat ggtgtcgtga tgggcacttc ccacgaagcc cccatgatgc gcggcatcga
     1261 agaatggaat cgtcatgccg ttgggcagac cacggatgcc aacggtaata ttaccaaccc
     1321 gggctccgac ccgtacggag gaaacgggtt atggcgcttc agcaagaacc ccgacgcgct
     1381 caagctgtat tgggccgacg gcatcaagcg catggtggat caagaattcg aaggcgtcgt
     1441 gacgcttggc atgcgcggcc ccggagacgt gagcttgccc gtcgaggacg gcatcgagct
     1501 gatgaacagc gtaatcgcgg cacagcgcgg catcctcgaa gatgaaaccg gcaaacccgc
     1561 gtgggcggcg agtccccaag tctggacctt gtacaaagag gtccaggact actgggacaa
     1621 aggcatgcgt gtgccagagg acgtgaccgt tgtttggtgc gacgacaact ggggcaacat
     1681 gcgtgaattg cccgttcaga gtgaagccaa gcgttcgggg gggtacggct tgtactacca
     1741 cttcgactac gtgggcggcg ggcgcaacta caagtgggtc gatacgaacc tgctgccgaa
     1801 catctgggag caactcaacc tgagctactc gtacggcgtg aaccgcttgt gggtggtgaa
     1861 cgtgggtgac atgaagaacg aggagcaccc cttggagttc ttcatggact atgcgtggag
     1921 ccccgagcag tggccgctcg agcgcctggc cgactggcaa gagcaatggg ccgcgcagca
     1981 gttcggagcg gattacgcgg tcgcgatcaa ggacatcctc catgactaca gccggcttca
     2041 gtcgctgcgc aaacccgagc tgaccaaccg atcgatcacg ttgaacccag ccaaggatat
     2101 ttcggatccg gcccagacgg gcgacgcggt ggtgtacgac gacgccgctt ccccgttcag
     2161 cctgctcaac tatcgagagc tcgagacgct ggcggaggac tggaagaacc tcgcgcagga
     2221 cgcggctgtc gtccgagact cgctgcccgc agagctgaag gatgcgtact atcagctcgt
     2281 gttttaccag gtgcaggcga ccgcttacct gtacgagcac cgcctggccg tgttcaagaa
     2341 caagctgtat caggcgcaag gccgtgcttc cacgaacgac tggggaacca aagcccaggc
     2401 cgccttcgac gagggtcgca agttggccgc ctactacaac gacaccttgg ccggcggaaa
     2461 gtggaagggc tggcagacgc agccgtacat cggttacggc gatgtagagc gctatggctc
     2521 gaacgccggc tggcagcagc cggagctcaa caatgcggcg atcgaggacg ctccgtatcc
     2581 gcacatcgtg acgaacttca cacccgtgac tggccgcagc atcggcgtca gcgtcgaagg
     2641 ttcggacaag gtgtggccga ttgaaactgc gaccaccgcg caactcccga agatcagccg
     2701 ctaccaaacc cagccgaatc catatatcga gatcttcaat cgtggggatc tgttcacgga
     2761 gtacaccctc aacatcccgc agaacgcaca gagctggctg tccgtgacga atgctgcggg
     2821 tcgcgtgggc aacacgaaca aggaagttcg cgcggaagtt caggtcagca actgggccaa
     2881 cgttcccgcg ggaacgaccc aggtcacgct gacgttgacg ggcaccggcg tcggcacgcc
     2941 gatcccggtc aacgtggtgg tggagaacac ctctgtgccg gctgacttcc gcggtttcgt
     3001 ggagggcggt ggctacgtag cgatgctggc cgagcactac tcccgcaagg tcgacagcgg
     3061 cgcggtgggc ttccgcttgc tgccggacat cggacgtctg agctccggct tgacgccctt
     3121 cccggtgaat gcgcccccgc agacacccgg cgatgcggcc gctccccact tggaatacga
     3181 cgtgtatctg accagcgccg gcgaaacgac gctgacctcg tacctcagtc cgcgcaacaa
     3241 cgtgctgcat tcggacggcc tcaagtacgc cgtttcaatc gacaacggca cgcccgaaat
     3301 cgtgaacatc accaaagccc tgaacggtat tccaatgaac cgttcttggg agcgcaacac
     3361 ctccgacaac gtcaaccgca ccacgaccaa gcacacggtc acggccggca aacacaccat
     3421 caagttctgg atggtcgatc ccacgatcat cgtccagaag ctggtgttgg ataccggggg
     3481 cgcacgcccg agctacttgg gtccgccgga gagcttccgg aaaaccaact gagtgatctt
     3541 ggccccacct cggcttttca ctgctgcgat gacccgcacg gctcacgcag ggcagtgcgg
     3601 gtttcggtgt gaagccgagg tttcggatac gagcggcggt tcccattctg ggacgccgct
     3661 cgcggcctat ttggagtttg tcgggcttcg tacctcgtga actggaatcg cgggctcgtg
     3721 acatccatga ggcacgaagc ccttttccgt ccgagccgtc acttttgccc gaactgagtg
     3781 cgccttggca gggtcactca gcgggctctt attgcagtcc gaatccctac ccgttcgatg
     3841 ccacagaatc gatatcttct tgaaggagcc gatatgaaac ccctaatccg ccctgggaat
     3901 accgttgcag agcttgccgg cgattccaga tcccatctcc cgatcacacg aggacacgac
     3961 gcgggctccg cccgcaggcg tggtgtcttg acccgcaatt tgacggctct tcattggggc
     4021 gcgctgccgg tcgtgctgtt cgttctgtct gcctgcggag aagagggtag ctccgggtgg
     4081 gccgcaggtg cgccaagcct gggcggggct cgcagcagcg gtggggctgg cggtagtaac
     4141 gggggcgcca cgagctcggg gggcacgtcc agtacaccgg gtggcacgac gagtgtcgcg
     4201 gggagcaccg cggttcaacc cggcgtcgat ccgtgggact ccaagcctga tcgcggcgtc
     4261 gtcaaagatc cgggacgcta cgtagcgact gaagacggag ccggccggtt cccactggtg
     4321 tcgaaccgca aggccgtgcc gatcgtgatg agtggcaagg acttttgggg ggtgcagcgc
     4381 accgccggtg acctcaaggc cgatatcgag cgcgtgacgg acgtcgaacc cgagctcgtc
     4441 agcgatacac ccccgagcgg tgccaaggac atcgtgctgg tgggcacgct gggcaagagc
     4501 ccactcatcg atcagctggt caaagacaag aagctcgacg ttagcgacat cgaggggcgc
     4561 tgggagacgt tcctgattca ggtcgtgcag aagcccatgg cgaacgtgga tcgcgccctg
     4621 gtcattaccg gcagcgatca acgcggtacg agctttggca tctacgacct gtcgaagcag
     4681 atcggggtgt caccctggta ttactgggac gatgtgcccg cgcgcaagag cagcgcgctg
     4741 tacgtgctac cggggcatca cacgatgggt gagcctgcgg tgaagtaccg cggcctgttc
     4801 atcaacgacg agaatccgca gcttggaact tgggcataca acaccttcgg ggcttccccg
     4861 aacgcgagct tcccgcaggc attccacaag aagatgtacg agaaggtctt cgaggtgatg
     4921 ctgcgcatca aggccaatta cttgtggccg gcggtttggg ggcgcgcctt cgccgaggat
     4981 gatcccgaga accacgccac cgccaaggct tatggcatcg tgatgggcac ctcccatgaa
     5041 gcgcctatga tgcgcggcat cgaggagtgg aaccgccacg ccgtccccga ggtgagcgac
     5101 gccagcgggg tggtcacgac gcagggcatg gatccatacg gcggaaacgg gctctggcgc
     5161 ttcagcaaga acccgaacgc gctcaagggc tattggaccg acggcatcaa gcgcatggtc
     5221 aaggaagact tcgagggcgt gatcacgctc ggcatgcgcg gcccgggtga cgtgagcctg
     5281 cccgtagagg acgggattgg cttgatgaac aacgtcatca aggagcaacg cgcgatcatc
     5341 gcggccgaga ccggcaagga cgtcacggcc ctgccccaag tttggacgct gtacaaggaa
     5401 gtccaagact attgggacaa gggcatgcgc gtgccggacg acgtcacggt cgtttggtgc
     5461 gacgataact ggggcaacat gcgggagctg ccgctgcaga ccgaacccaa gcgctggggt
     5521 ggatatggtc tctactatca cttcgattac gtgggtggcg gtcgcaatta caagtgggtc
     5581 gacaccaacc tgctgccgaa cgtctgggag cagctgaatc tctcgtactc ctacggcgtc
     5641 aaccgcttgt ggatggtgaa cgtcggagac atgaagaacg aggagcatcc gctcgagttc
     5701 ttcatggatt acgcgtggag ccccgagcgt tggccgctcg agagcttgcc tcagtgggag
     5761 cgccaatggg cagctcaaca gttcggtgag gagtactcag tcgcgatcgc ggacgtgctg
     5821 cacgcttatt cgaagctgca gtcacgccgc aagccggagc tcaccaaccg ctccatcacc
     5881 ctcgatccgg ccaagaatat ctctgatcct ggtcagacgg cggatgcggt caagtacgac
     5941 gataaggcct ctccgttcag cctcaccaac tatcgcgagc tggagcgcat ggtcgaggat
     6001 tggaatcagc tcgcgagcga agcggtcacg attggagagt cgctgcccaa ggaagcgcga
     6061 gacgcatact tccagttggt gctgtaccaa gtccaggctt cggcggagct ttacaatcac
     6121 cgcctggccg tgttcaaggg cctcttgtac aaggctcaag gtcgctggtc caccaacgac
     6181 caaggcgcca aagctcagga gttcttcgac aagggcaccg cgctcgcgaa gatgtacaac
     6241 gagacgatcg cgggcggtaa atggaagggt tggcaaacgc agccgtacat cggctacgga
     6301 gataccgagc gctacgggtc gaatgcctcg tggcagcaac cggagaagga taacgcggct
     6361 ttgccggatg caccgtatcc gcagatcgtg accgatttca tggttacgga cggcaaagac
     6421 gtcggagtaa ccgtcgatgg ctcggatcag gtgtggacgg ccaagacgcc cgcagaaacg
     6481 ccgcccgcct ccctgccgac cttcagtcgc taccaagtcc agccaactca gtatatcgag
     6541 gtcttccgga ccggttcggg tacggacgaa gtcccattca gcatcacggc tcccgccgaa
     6601 gccacttggc tgaccatcac tccgtctacc ggcactctga acgcaaccca aaaagacgcg
     6661 cgaatcgagg tcaaggtcga ttggaccaag gcgcctgcgg aggcgcagac agtcaagctg
     6721 acggtcgcca ccggggcgag ctcgagccgt accacggtga cgaccgtcaa cgccgtaatc
     6781 gaccccaagc tcgtgcccga gactttcagc ggtttcgtgg aagcgaacgg gtacgtgtca
     6841 ctcgaagcgg atcactttag ccgcatcatc gacgggccga gtgtcggctt ccgcaagctt
     6901 cctgagattg gccgaaccgg ctctgggatg actcccttcc cggtgaccgc ggccgcacaa
     6961 aagccaggcg gtacgtcgcc acatctggag tatgacgtct acctcggtac gggtggcgac
     7021 atcacggttt ggacgttctg ctcgccgcgt aacaacgtgc tgcacggcga cggtttgaaa
     7081 tatgcgatct ccatcgacga cgcggagccc cagatcgtga acatcaccac ggcgttgaac
     7141 gggattccga tgaacaagtc gtgggaacgc aatacctcgg acaacgtgaa ccgcacctcg
     7201 gtcgcgctca agggggtaac gccgggcgcg cacgtcctga agctctggat ggtcgacccc
     7261 accgtggtcg tacagaagct cgtggtggac accggcggtt tgaagccgag ctacctcggt
     7321 ccactcgaga gccaccgcat tacgcgctga ttgtcgtagt tcgaagtaga ggaaagtcga
     7381 agcgagagag cggtcactcc ggttttggag ggccgctctt tcttttcgtg gtcagctcac
     7441 ggtccccgtc tgcaggcggc acgccaatcc ccccattcga cgcaatcgca tcactcgagg
     7501 gctcacccac attcggcgtg tgcttggcgg acggcgaaag aacgaatctt cggtgcgact
     7561 acgcagttcg ctcacttgaa tggattgtct tgggagagcc cgtaggagct ggctcgggag
     7621 cgggggatcg aggtgcccgg agacccggcc gccggaacac ggttggacga ggaagcgggg
     7681 ccagagtgcg gtcgagccgg gcctgggcga gcgctcgcga tgacgctggc cgcgggttct
     7741 acgctgctgt ttccgatggc gctcgtggcg tctatctctt ccgtccgtgc gggggcttca
     7801 ggagccgagc tgggcgaaag cttcgacgga ggcgaggttg agctcgacgc cgaagcaagc
     7861 gcggacgcgt cggggatggg gttcttggcg tcgctcgtca agccctgggt gcggtccgac
     7921 gcagagcgtt cgcgcagggc gatagtcaga cccgagacga ccaagagcgc gccaaccgca
     7981 gcgacgatga accaagggcg ggtaactcgc ccgcccgcgg gggctcgcga actcgcgtca
     8041 tggagcggtg tcgaggcgct ggccaacgag cccgggttac cgagctgggt agggctcaac
     8101 gcgataggag atatggagtc acgcagtcgc gcgatggccc tcgagaactc agccgcgctc
     8161 tcgtagcgat cggcgcgttc actcgccatg gcacgcgata cgacgaggtc gacggcagcc
     8221 ggcaagccct gtcgcacctc gctcggggtt gggtacttgc cttgatgaat cagtaccgcc
     8281 aaatgcggca gcgtgtccgc gacgaagggc ttgcgaccgg tcaggcattc gtagagcagc
     8341 acccccaagg catagatatc ggctcgccca tcgaggctgc gttcaccgcg gacttgttcc
     8401 ggggacatat aatacggagt gccaatcggg gaaccttcca aggtaagccc gtgctctccg
     8461 gtcgtggcga cgtcgaactt ggacaccccg aaatccagga tcttgacgaa ggacaaggcg
     8521 gggccggtca aaaacagatt ttctggtttg agatcgcgat gcacgatgcc caccgcgtgg
     8581 gcggcgctga cggcgtcaca cgcttggatc aggatgtcgc aagtggcatc gatgggcagc
     8641 ggcccctgct cgatgagcac ctcggacaag gtcttacctt gcagcagctc catcactatg
     8701 tacggttcgc cgctgtcgag acgacccgca tcgaacgtct ctacgatgtg agcatttccg
     8761 attcgacccg cggccgaggc ctcgcgcagg aaacgctcca ccacggtcgg gatgcgggcg
     8821 gcgtgcgcat gcagcaactt gagggcgcgg cgatggtgcg tgagttcgtg ctcgacctcg
     8881 tagaccgccc ccatacctcc gaaaccgagc cgccggatga tgcggagttt tcctccgagc
     8941 agggtccctg ttgcgaaacg atccggcgcg aaacgatcac ttggcttttg gtcgacggag
     9001 ccggtgtcgt cgtcccgatg gtcgctctgc atgatgcacc gaaggccctg gcgagggtat
     9061 cacggttcgt gagactattc ccgtaaacca gtaccctgcc tggagcccat gcgcgcacgc
     9121 ctctctggac tacgcatccc tgtcgttctc tgttctatcg ccagtttgtg cgctcccgcg
     9181 ctcactctgg ctcagcccgc cccgtcggct gagggggcgc agcaaagccc ggaagcagct
     9241 caacaacgcc gggcggaagc agccgagcgc ttcgatcgtg ctttgaagct gttcgaagag
     9301 gggaacaacg cgtctgcgct agctgagttt cgccgcgttc aggagatctc cccgaacccg
     9361 atagtacttt ataacatcgg gttggtttcg gcggccatgg gccgcgccgt cgacgcagtg
     9421 gacgcgctca ccgaggtact gaagaatccc gaagcgctcg gtcccgcgcg ggccgcgaga
     9481 gctcgcgtcg cgctcacgga gcagtcgacg cgcatcgcgt tactcgacgt gtcggtgaac
     9541 accgaggtcg ctcgcatcga ggtcgatggc gtggaggccg ggcgcgctcc gctatcggcg
     9601 ccactgcgcg tcaccagcgg ctctcacttg gtgggcgcgg tcgcagaggg ctacgtgccc
     9661 gaacggcggg aggtcttggt ggctggcgga gcgatcacac ctctgcggtt cgagctggtg
     9721 gagatgcgcg ggcgaaggct cgcgaacttg agcttgcgca ccaacgtatt ggccgccgag
     9781 gtcgtgatcg acggggcagt cgtggctcgc acgccgctcg ccgcgaccct cgcgctcgct
     9841 ccggggcagc atcgcatcga gctgcggcgc gcgggttacc agcctcgcgc gcagacactc
     9901 gagcttggtg atggcgcgac cggcgaattg acgtggccgc tcgagatcga tccggccgca
     9961 ctcacgagcc aaggcgcaga cgttacgctc gagatctcgg agccgggagc ccagctcagc
    10021 gtggacggag tctcgagcgg gctttacgcc ggaccgttgc gcttgcccgt ggggccgcac
    10081 catgtacggg tcgagcgcgc cgggttcctg agttgggagc gggtgatcca ggtcgacccc
    10141 cacaaaccca acggggtgaa cgtcgagctc gagccaaccc ctcagactcg cgccgaggtc
    10201 gctcgcaacg cgcaccaaca tcggctctgg ggaatcgtcg gcgtggcagg cggcgccgtg
    10261 attgccggcc tcggcacggg ctggttgctc cacaatggcg ctcgcaagag cgacgctagg
    10321 caggccgtcg acgctatcaa cgccgaggcg agcagcgatt ccggccgttg cgataccagc
    10381 agcggagccg ttacccaaga gcaatgcaac gcgagtgtcg atgccgctta ctccgagtgg
    10441 aactcggtca agaaacggga tgtctacggc tggattggag taggcgtggg cgccgcggct
    10501 ctgacgaccg ggatc
//
LOCUS       DASTKU010000068         3144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_681879, whole genome shotgun sequence.
ACCESSION   DASTKU010000068 DASTKU010000000
VERSION     DASTKU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681879"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(421..561)
                     /locus_tag="VFQ61_02270"
     CDS             complement(421..561)
                     /locus_tag="VFQ61_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953297.1"
                     /translation="MGTPQDPRARRRQRIRRAKKNMEWELRRAQENAEAAKPKTPAKT
                     TT"
     gene            complement(602..1624)
                     /locus_tag="VFQ61_02275"
     CDS             complement(602..1624)
                     /locus_tag="VFQ61_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HET9953298.1"
                     /translation="MSNIVEPSRLTKKDFETDQDVRWCPGCGDYSILANVQRVMPELG
                     IPPERIVWVSGIGCSSRFPYYVNTYGFHTIHGRAPAFATGIKIANPELSVWLATGDGD
                     GLSIGGNHLMHCLRRNVDVKILLFNNRIYGLTKGQYSPTSEFGKITKSTPFGSADQPV
                     DPSAFALGANGTFVARSIDTEAAHLSDVLRRAARHTGTAFIEIYQNCNVFNDGAFDAL
                     KEKPQSLETQLRVEHGQPLLFGANRDRGLKLNTKTLNLEVVRIGEDGVTLDDIVVHDE
                     TNATLAQMLARLSLPEFPVALGVLYAVHRPTYDGSVRAQREAAQARGNAPDLARLLRG
                     GETWEI"
     gene            complement(1643..>3144)
                     /locus_tag="VFQ61_02280"
     CDS             complement(1643..>3144)
                     /locus_tag="VFQ61_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HET9953299.1"
                     /translation="GANLKKAGYEVNPLESGSLEGYQLLSIDITKQTQNAVKEAGLSN
                     KDANRCKNFWTLGLRFWIYGRPMEPTLRWIAQKFGKRPEVATANELALKAGHAYGETA
                     EIFQHRYEVPAAPVEPGVYRNISGNAATALGLVAAAQLAGRELVLGAYPITPASDVLH
                     ELSKHKSFGVTTLQAEDEIAAICAAIGASYAGCIGVTSTSGPGMALKTEAMGLAITTE
                     LPLVIIDIQRGGPSTGLPTKTEQADLLQAVYGRNGEAPLCVMAAASPGDCFYMALQAV
                     RFAVKYMTPVVLLTDGYIANGAEPWKIPKVSDLAPIPVSFRTDPVGFHPFVRDEKTLA
                     RAWAVPGTPGLIHRIGGLEKDYDSGHISYSPENHERMSRTRAAKVAGMVADVPDQEIE
                     VGTDSGKLLVLGWGSTYGSLREAVQRCRERGLDVSHAHVRYLNPFPKNLGELLKRFDR
                     VLVPELNLGQLVKLIRSQYLVPAESFPKIQGQPFKISEIEDKIRRMLES"
BASE COUNT          549 a         1007 c          960 g          628 t
ORIGIN      
        1 aaagcaaagc taggggctcc cgcagctggc tagactgcag ccctgtttca caacgccttc
       61 acaacatccc catttcggtt tcgccccaga caggcggggc cgatcggacg tgaagaatcc
      121 gaaacctgcc gtgcacaacc cctatacggc agcggctgaa cccagccccc gtctggactg
      181 ggcaggcgct gcatcgacct agtcaacggg cggaagcgac gaacgagaca tttaacccaa
      241 gcgaagttcg ctccaggcat cgcgtctcgt ccgcgcgcac cgtgttttgc cgaggtactg
      301 agttttgccg agtcccgggt atcgctgaaa tctgagtccg tacacgtcta cacgcttctc
      361 gcatgtcatc cgcgcactgg aactctcgac ctcccgactt cggcgtctgt tcgaagcggc
      421 tcaggtcgtg gtctttgccg gggtcttggg cttggcagcc tccgcgttct cttgagcgcg
      481 gcgcagctcc cattccatgt ttttcttggc acggcgaata cgctgtcgcc gacgagcgcg
      541 aggatcttgc ggggttccca tggcggctgc cctttagcag gtttaatcga gcttgtctcg
      601 ctcagatttc ccaggtttct ccgccgcgca gcaaacgcgc cagatctggc gcatttcctc
      661 gggcctgagc ggcctcgcgt tgcgcccgaa cactcccgtc gtacgtagga cgatgcacgg
      721 catacagaac gccgagcgcg acggggaact ctggcaatga caggcgagcc agcatctgcg
      781 ccagcgtggc gttcgtctca tcgtgcacga cgatgtcgtc caaggtcacg ccgtcttcac
      841 caatccgcac cacctccagg ttgagcgtct tggtgttcag cttgagcccg cgatcacggt
      901 tggccccgaa caacaacggt tgcccgtgct ccactcgtag ctgcgtttcc aaactttgcg
      961 gcttttcctt caacgcgtcg aacgcgccat cgttgaagac gttgcagttc tggtaaatct
     1021 cgatgaaagc cgtgccggtg tggcgcgccg cccggcgcag cacgtccgac aaatgtgcag
     1081 cctcggtatc gatcgagcgg gcaacgaagg taccgttcgc ccccaaggcg aaggcggaag
     1141 gatccacggg ctgatccgcc gagccgaacg gggtgctctt ggtaatcttc ccgaactcgc
     1201 tcgtcgggga gtactgcccc ttggtgagtc cataaattcg attgttgaag agcaggatct
     1261 tcacgtccac gttgcgacgc aggcagtgca tcaagtgatt gccaccgatg ctgaggccat
     1321 cgccgtcgcc cgtcgcgagc caaacgctga gctcggggtt cgcgatcttg atgcccgtgg
     1381 cgaaggccgg cgcgcgacca tgaatcgtgt ggaagccgta ggtattcacg taatacggga
     1441 aacggctact gcagccgatc ccactgaccc agacgatgcg ctcgggagga attccgagct
     1501 cgggcatcac gcgctgcacg ttggcgagga tcgagtaatc cccgcaaccc gggcaccagc
     1561 gcacgtcctg gtcggtctcg aaatctttct tggtgaggcg gctcggttcg acgatgttcg
     1621 acatggcagg gttccagggc gctcagctct cgagcatgcg gcggattttg tcctcgattt
     1681 ccgagatctt gaagggctgc ccctggatct tcgggaaact ctcggcgggc actaggtatt
     1741 ggctgcggat cagcttcacg agttggccca agttcagttc gggtaccagc acgcgatcga
     1801 agcgcttcag cagctcaccc aggttctttg ggaaggggtt gaggtaacga acgtgggcgt
     1861 gagaaacgtc gaggccgcgc tcacggcagc gctgcaccgc ttcgcgcagc gacccgtagg
     1921 tggagcccca accgagcacc agcagcttgc cgctgtcggt tccgacttcg atctcctgat
     1981 ccggcacgtc ggcaaccatc ccggcgacct tggcggcgcg ggttcggctc attcgctcgt
     2041 ggttctccgg tgagtacgag atgtgacccg agtcgtagtc cttttcgagc cctccgatgc
     2101 ggtggatcag gcccggggtt cccggaactg cccacgctcg cgcgagtgtc ttttcgtccc
     2161 gcacgaacgg atggaacccg accggatcgg tgcggaaact caccggaatc ggggccaaat
     2221 cagaaacctt ggggatcttc cagggttcgg cgccgttcgc gatgtagccg tcggtcagca
     2281 acacgaccgg agtcatgtac ttcaccgcga agcgcaccgc ctgcagcgcc atgtagaagc
     2341 aatcgccagg ggatgcggct gccatcacgc acaacggcgc ctcgccgttt cggccgtaaa
     2401 ctgcttgcag caggtccgct tgttcggtct tggtcggcaa accggtgctc ggcccgccgc
     2461 gctggatgtc gatgatcacg agcggaagct cggtcgtgat cgccaagccc atcgcctccg
     2521 tcttcaaggc catgcccggg ccgctggtgc tggtcacgcc gatgcagccg gcgtaggacg
     2581 ccccgatggc agcgcaaatc gcggcgatct cgtcctcggc ctgcagcgtc gtgacgccga
     2641 aactcttgtg cttgctgagc tcgtgcagca cgtcactcgc cggagtgatc ggataagcac
     2701 cgagcacgag ctcgcggccc gccagctgcg ccgccgcgac gagccccagc gccgtcgctg
     2761 cattgccgct gatgttgcgg tacacgccgg gctcgacggg cgcggcgggg acttcatagc
     2821 ggtgttgaaa gatctccgcc gtctctccgt aggcgtggcc ggccttgaga gcgagctcat
     2881 tggccgtggc cacctctgga cgcttgccga acttctgcgc gatccaacgc agggttggct
     2941 ccatcggccg cccataaatc cagaacctca ggcccaaggt ccagaagttc ttgcagcggt
     3001 tcgcgtcctt gttcgaaagc cccgcttcct tgaccgcgtt ctgcgtttgc ttggtgatgt
     3061 cgatcgagag cagctggtag ccctcgaggc tgccgctctc gagcggattg acctcgtaac
     3121 cagccttctt caggttcgcc ccgt
//
LOCUS       DASTKU010000069         5621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_13686,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000069 DASTKU010000000
VERSION     DASTKU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13686"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFQ61_02285"
     CDS             <1..632
                     /locus_tag="VFQ61_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9953300.1"
                     /translation="EAYAERLPTVEINNTFYRMPDAKLLEGFRDRVPEGFTFSLKAPR
                     SITHSAKLRNVESKVQQFVGLAQLLGTKLGPLLFQLPPFLKKDLALLTDFASILPKGV
                     RAALEFRHPSWFDDEVYETLRRHALALVATEVDEGDGIDSPYVATAPFGYVRLRRQNY
                     DAQSLKAAARNIENLRVEPVFAYFKHEVLGPGYADQLARIEYELPDSPE"
     gene            721..1545
                     /locus_tag="VFQ61_02290"
     CDS             721..1545
                     /locus_tag="VFQ61_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953301.1"
                     /translation="MATCGLVACTLDACAPALSSFTPAHVAAPGHVQAEVGTDVSVPR
                     NTLADLYDITRALDRAAQNRTLTQTEQRQLLRGATSQLLNPLSANSHLGLGVGVYRNL
                     EAQGRLFTGGWRLGARLQFLSQPEHGLDASVGLGVGRYSYSFGIPNIADVLEIEDYGR
                     WMVDVPVLVGKRGDWYRWWGGPKALLGTYRAGASLNLPAENKSYGFDLRGTSGYVGAQ
                     LGGALGYKHLFFAAELSVMYFTTRARFTTNGVALEPYEARIDGLIWYPGVGLLGEF"
     gene            1612..1998
                     /locus_tag="VFQ61_02295"
     CDS             1612..1998
                     /locus_tag="VFQ61_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9953302.1"
                     /translation="MIDHTGVNVSDPVKSRRFYEGALAPLGYKVLVELPLEVTKGAVV
                     LGFGVPPKADFWIQQGTPQTPRNHMAFTAADRAQVDAFYEAAMAAGGRDNGPPGLRPH
                     YHANYYGAFVLDPDGHNIEACCHDPV"
     gene            2044..3318
                     /locus_tag="VFQ61_02300"
     CDS             2044..3318
                     /locus_tag="VFQ61_02300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9953303.1"
                     /translation="MHHSSPHWESPRRALRAWTLSWLAYAIYYVGRKGFSVSKKTMAA
                     EFGLSEATLGGIDTAYLIAYTVGQFTSGWLGDRIGARRLLGYGLLGSGLACAAFGFGS
                     SASVFAVSFLINGLFQATGWPGAVRAMSEWTTPKNRGTVMAFWATCYQVGGVVANALC
                     AYFLVHWGFRSAFWGPALILGSMGLALLRWLEAGRSEQTPLGSAGSEQPASAAKDQVP
                     SAGPHVSAAQLHLLRNPFLWSYGASYFFIKFIRYTLLFWLPYYLSTQHAYAADVAANV
                     SSAFELGGVVGVIALGTLSDRLPALSRAVWSALSLLGLAAALFLYIELASRGWFWNAA
                     GLALIGALLFGPDALLSGAAAQDAGGARAAAMAGGMVNGLGSLGAIAEGLVVPRLSAR
                     LGWPALFPTLVVLALAAAFCLLPARRLRASTD"
     gene            3598..5121
                     /locus_tag="VFQ61_02305"
     CDS             3598..5121
                     /locus_tag="VFQ61_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9953304.1"
                     /translation="MIYAQPNQAGSKVSFRSRYGNYIGGEWKEPAKGRYFENVTPVTG
                     RAFCEIPRSTSEDIDSALDAAHRAKESWGRTSPTDRAILLNRIADRMAENLERLAVAD
                     TWDNGKPIRETLAADLPLAIDHFRYFAGCLRAQEGALSQLDDNTVAYHFHEPLGVVAQ
                     IIPWNFPLLMAAWKLAPALAAGNAVVLKPAEQTPASILVWLELVQDLLPPGVLNVVNG
                     FGTEAGKPLASSPRVAKVAFTGETTTGRLIMQYASENLIPVTLELGGKSPNLFFEDVL
                     AASDSYLEKALEGFTMFALNQGEVCTCPSRALIAHGIQGRFLELALDRTRKHAVPGNP
                     LDPQTTVGAQASRDQLEKTLSYIDIGKKEGAKVLIGGERARLSGDLAEGYYVMPTVFA
                     GHNRMRIFQEEIFGPVVATTSFKDFDDCIQLANDSLYGLGAGVWTRDLNVAYRAGRAI
                     KAGRVWTNCYHLYPAHAAFGGYKQSGIGRENHKMMLSHYQHTKNMLVSYDAKPMGLF"
     gene            5172..5591
                     /locus_tag="VFQ61_02310"
     CDS             5172..5591
                     /locus_tag="VFQ61_02310"
                     /inference="COORDINATES: protein motif:HMM:NF017426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HET9953305.1"
                     /translation="MDIPRVEVTDQARALIRELRNEHGPLMFHQSGGCCDGSAPMCFL
                     RGEFRVGAQDVRLGDIEGCPFYMSVAQFDYWRHTQLIIDLVPGRGAAFSLEAPRGVRF
                     LTRSRAFSEAELAELARQGEPPRGDSEAPVETWAPFT"
BASE COUNT          969 a         1730 c         1820 g         1102 t
ORIGIN      
        1 tcgaggccta cgccgagcgc ctgccgacgg tggagatcaa caacaccttt tatcggatgc
       61 ctgacgcaaa actgctcgag ggcttccgcg accgcgtccc cgaaggtttt acgttttccc
      121 tgaaggcccc gcgtagcatt acccacagcg cgaagcttcg aaacgtcgag tcgaaggttc
      181 agcagttcgt ggggctcgcg cagttgctcg gcaccaagct cggaccgctg ctctttcagc
      241 tcccgccgtt tctcaagaag gacctcgcgt tgctcacgga cttcgcgagt attctgccga
      301 agggagtgcg ggcagcgctc gagtttcgcc acccgagctg gttcgatgac gaggtctacg
      361 aaactctacg tcgtcacgcg ctggccttgg tcgccaccga agtggacgaa ggtgatggca
      421 tcgactcgcc ctatgtggcg acggcgccgt tcggttatgt gcgcctgcgc cggcagaact
      481 acgacgcgca gagcctgaag gcagccgcgc gtaacatcga gaatctgcgc gtggagccgg
      541 tttttgccta tttcaagcac gaggtactcg gccccggcta cgccgatcaa ctggcacgca
      601 tcgagtatga gttgcctgac tcgccggagt gaccgcgccg cccgcgacct cggcgccact
      661 cgctcgaagt gcgagacgcc ttctttgcga tcagcagcgt tctggcgacg cgcagctcgg
      721 atggcgactt gcggcttggt cgcttgcaca ctcgatgcgt gcgcacccgc gctctcttct
      781 ttcacgccgg cacacgtcgc cgcgcccggg cacgtacaag cggaagtggg cacggatgtc
      841 tccgtgccca gaaacacgct cgccgatctg tacgacatta cgcgagccct cgaccgcgca
      901 gcccagaacc gcacgctcac tcagaccgag caacgccagc tgctacgggg cgcgacctcg
      961 cagttattga acccgctgtc cgcgaattca catctcggcc tcggcgtcgg cgtgtatcgc
     1021 aacttggaag cgcaaggccg tcttttcact ggcgggtggc gcttgggtgc gagactccaa
     1081 ttcctgtccc agccggaaca cggactcgac gccagcgtgg gactcggcgt cgggcgatac
     1141 agctatagct tcggtattcc caacatcgcg gacgtgctcg aaatcgagga ctacgggcgc
     1201 tggatggtgg acgtgcccgt gctcgtcggc aaacgcggcg attggtatcg ctggtggggt
     1261 ggaccgaaag cgctgttggg tacctatcgc gccggcgcaa gcctgaatct gcccgcagag
     1321 aacaagagtt acggcttcga cttgcgcggg accagtggtt acgtgggtgc gcagctcggc
     1381 ggtgcgctcg gctacaagca ccttttcttc gcggccgagc tgagcgtgat gtacttcacc
     1441 acccgggctc gcttcacgac caacggcgtc gcgctcgagc cgtacgaagc gcggatcgat
     1501 ggattgatct ggtaccccgg cgtcggcctg ctcggtgaat tctaaggtac ggctcgatct
     1561 gcgcggcgat cgtctcgccg aggcggacgt ggttcgtgat aaaagcggct catgattgac
     1621 catactggag tgaatgtcag cgatccggtc aagagtcgtc gcttttacga aggtgcactc
     1681 gcgccgctcg gctataaagt gttggttgag ttgccgctag aggtgaccaa gggagcggtc
     1741 gtgttgggct tcggagtccc ccccaaggct gacttctgga ttcagcaagg cacgccacag
     1801 acaccgcgca atcacatggc gttcacggct gcagaccgcg cgcaagtaga cgcattctac
     1861 gaggccgcca tggcggccgg cggccgagac aacggccctc ccggcctgcg cccgcactac
     1921 cacgcgaact actatggtgc gttcgtactc gacccagacg gtcataatat cgaagcctgc
     1981 tgtcacgatc cggtctagcc cgccctcacc acgctatcta gagtgccgaa ctctcgttct
     2041 cgagtgcacc actcctctcc gcactgggag tcacctcgac gcgcgttgcg cgcttggacg
     2101 ctgagttggc tcgcttacgc gatctactac gttggacgca aaggcttcag cgtctccaag
     2161 aagacgatgg ccgccgagtt cgggctgagc gaagctactc tcggcggcat cgatactgcc
     2221 tatctcatcg catacacggt agggcagttt accagcggtt ggctcgggga tcgcatcggg
     2281 gcgcggcgcc tgttgggcta cgggcttttg ggctcgggct tggcttgcgc cgcgttcggc
     2341 ttcggaagct cagcgagcgt cttcgcggtg tcgttcctga tcaatggcct gttccaagcc
     2401 acgggttggc caggggccgt acgggcaatg tcggagtgga caacgccgaa gaaccgcgga
     2461 acggtaatgg cgttttgggc aacgtgttac caagtcggtg gcgtggtcgc caacgcgctc
     2521 tgcgcctatt ttctcgtgca ctggggcttt cggagcgcgt tttgggggcc agctctgatt
     2581 ctgggttcga tgggtctggc tttgctgcgt tggctcgagg cgggtcgctc cgagcagacg
     2641 ccgctcggct cggccggaag cgagcaaccc gcgagtgccg caaaggatca ggtcccgtcc
     2701 gctgggcccc acgtatccgc cgcccagctg catctgctgc gcaacccctt cctttggtcg
     2761 tacggggcct cgtacttctt catcaagttc atccgctata cgctgctctt ctggttgccg
     2821 tactacttgt cgactcagca tgcgtacgcg gcggacgtcg cggccaacgt ctccagcgct
     2881 ttcgagctcg gcggggtggt cggcgtgatt gcgctcggca cgctttcgga ccgtttaccg
     2941 gccctatcgc gcgcggtttg gtctgcgctc agcctgctgg ggctcgcggc ggcgttgttt
     3001 ctatacatcg agttggcgtc tcgagggtgg ttttggaacg cggccgggct cgcgctgatc
     3061 ggcgcgctgt tgttcgggcc agatgcgttg ctatcgggcg ccgccgcgca agacgcggga
     3121 ggcgcgcgtg cggccgcaat ggccgggggc atggtcaacg gcctcggctc gctcggcgcg
     3181 atcgccgaag gtttggtggt accccgcttg agtgcgcgct tgggttggcc cgccttgttc
     3241 ccgaccctgg tcgttttggc attggctgcg gcattttgcc tgctgccggc gagaagactt
     3301 cgggcgtcca ctgactagcc ttcactagtc accagcacga gcacgacctc acgagggcag
     3361 cctcacgagg gcagtctggt accaggcccg aggtgcacca ggtacctaca gagtgtgcag
     3421 aaactctgtg tacgccgctc gcgcgtgaat ggagttatgt tcgttgagaa gcgccacccg
     3481 gggcgacaac attcgcgatg ttgtttcgtc ccttcgtgct gagggatcgg gatcgagcgg
     3541 tgggcttcgc gtcgagctcg cacgcgccac gcgcatcaca tcaggaggga gtccgtcatg
     3601 atttacgcgc aacccaatca agccggttcc aaggtgtcgt ttcggtcacg ctacggtaac
     3661 tacatcggtg gggagtggaa agaaccggca aagggccgct acttcgagaa cgtgacgccc
     3721 gtgacgggcc gtgcgttctg cgagatcccg cgctcgacga gcgaagatat cgacagcgca
     3781 ctcgacgcgg cgcaccgggc caaagagagc tgggggcgaa catcaccgac ggatcgcgcc
     3841 attctcctga accggatcgc cgatcgcatg gcagagaacc tagagcggct cgcggtggcc
     3901 gacacctggg acaacggcaa gcccattcgg gagacgttgg ctgcggactt accgctcgcg
     3961 atcgatcact tccgatactt cgcgggctgc ctgcgtgcgc aagagggtgc gctgagtcag
     4021 ctcgatgaca acacggtcgc ttaccacttc cacgagccgc ttggcgtcgt agcgcagatc
     4081 atcccctgga acttcccgtt gctgatggcg gcttggaagc tcgcgcccgc tctcgcggcg
     4141 ggcaacgcgg tggtactcaa gcctgccgag cagacccccg cctctatcct agtttggctg
     4201 gagttggttc aggacctgtt gccgccaggc gtgctcaacg tcgtgaatgg cttcggtacc
     4261 gaggcgggta agccgctcgc ctcgagccct cgcgtggcca aggtcgcgtt cacgggagaa
     4321 accacgactg gtaggctgat catgcagtat gcgtcggaga acttgatccc cgtaacgctc
     4381 gagctcgggg gcaagtcgcc gaatctgttc ttcgaggacg ttctcgcggc cagtgacagc
     4441 tatctggaaa aagcgctcga gggcttcacg atgttcgcgc tgaaccaagg cgaagtctgc
     4501 acgtgcccat cgcgcgctct gatcgcacac ggcatccaag gtcgattcct ggagctggcg
     4561 ctcgatcgca cgcgcaagca cgcggtgcct ggcaacccgc tggatccgca aacgacggta
     4621 ggagcgcaag cttcgcgaga ccaactcgag aagacgctca gctatatcga tatcggcaag
     4681 aaggaggggg ccaaggtgct gattggcggg gaacgcgcgc ggctctcggg ggacttggcc
     4741 gaaggttatt acgtgatgcc caccgtgttc gccggccaca atcgcatgcg cattttccag
     4801 gaggagatct tcggtcccgt cgtggcgacc acgtctttca aggatttcga cgactgcatt
     4861 cagctcgcca acgactccct gtatggcctc ggggcggggg tttggacacg cgatctgaac
     4921 gtggcgtatc gcgcggggcg ggcgattaag gcgggtcgcg tgtggaccaa ttgctatcac
     4981 ctgtatccgg cccatgcggc tttcggcggt tacaagcaat ccgggattgg ccgcgaaaac
     5041 cacaagatga tgctatccca ctaccagcac accaagaaca tgctggtcag ctacgacgcc
     5101 aaacccatgg ggctgttctg atgcgcggct taacgggttc tcggttgttg tcgaggcatg
     5161 aggaggcgca agtggatatt ccgcgagtgg aggtgaccga ccaagcgcgc gcgctcattc
     5221 gagagctgcg caatgagcac gggccgctga tgttccacca gtccgggggt tgctgcgacg
     5281 gcagcgcccc catgtgcttc ttacgcggcg aatttcgggt cggggcgcag gacgtgcggc
     5341 tcggggatat cgagggctgc ccgttctata tgagcgtcgc gcagttcgat tattggcggc
     5401 acacgcagct gatcatcgat ctggtcccgg gccgcggagc ggcgttctcg ctcgaggcgc
     5461 cgcgcggggt gcgctttctc acccgctccc gcgccttcag cgaggcggag ctcgccgagc
     5521 tcgctcgaca gggtgaacca ccgcgcggag actccgaagc gccggtcgaa acttgggcgc
     5581 cgttcacgtg agccatggag cgcaggagaa acgtggcggc t
//
LOCUS       DASTKU010000070        12244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_235488, whole genome shotgun sequence.
ACCESSION   DASTKU010000070 DASTKU010000000
VERSION     DASTKU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12244
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235488"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /gene="pgk"
                     /locus_tag="VFQ61_02315"
     CDS             complement(<1..499)
                     /gene="pgk"
                     /locus_tag="VFQ61_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET9953306.1"
                     /translation="MTTQKKLFIEDINPSGKRVIVRVDFNVPIKDGVVESDKRLRESL
                     PTIKYLAGKGAKVILMSHLGRPDGKRVPEMSLAPVAKALSDLLGSPVAFANDCVGDVA
                     KQAVAALAPGSVLLLENLRFYAEEENNDEAFAKQLSELADVYVNDAFGTAHRAHASTA
                     GITRFT"
     gene            complement(586..1569)
                     /locus_tag="VFQ61_02320"
     CDS             complement(586..1569)
                     /locus_tag="VFQ61_02320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9953307.1"
                     /translation="MKILLADDEPIARTMLEHWLTGWGYKVTTVKDGEAALAALKADP
                     EIHLAVVDWVMPKVDGPAVCRTVRAGPSEPYVYLVLLTARDNKADIVAGLDAGADDYL
                     IKPCNPLELKVRLRAGSRVVELQQQLVAARESLRFEAMHDGLTGILNRRAVLEHLERE
                     LARSQRKGGPLAVLMADLDYFKKVNDTYGHEVGDVVLKEAARRINTCVRAYDSLGRMG
                     GEEFLAVLPDCGGGMALSVAERVGAAVAQVPVETPVGPVPISVSIGVASTEQVEAVAV
                     SDLTRAADAALYRAKHAGRARACLAVPAEFAVKRSGEQAFPSGISVARARA"
     gene            1732..3096
                     /locus_tag="VFQ61_02325"
     CDS             1732..3096
                     /locus_tag="VFQ61_02325"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET9953308.1"
                     /translation="MLRQLRALLLFWTVFASYGFEWLLSRVLSEAVVRRRMEHAHRKN
                     ARRLAEGFSRLRGVFIKMGQVLSVMGTFLPSAYGEELEKLQDKVPPRPFREVELRLTE
                     AFGEAALSRFGSFEETPIAAASLAQVHRATTRAGEVLAVKILYPGIEQLIRRDLSVLR
                     SLLPVVRRLLPISRVERNLDQLEAMLARETDYAHERANILQMKGTFANRQDIIVPTVI
                     DELTAGGVLTMSFETGLKVTDTAAQREQGIDPEAVARLLVDAYFVMLFDHGIFHADPH
                     PGNFLVRPGPKLVVLDYGAVENVTPALSDGMKMVVLGALTRNDDQILMGLERMGFVAQ
                     GGDRELLSRVGREYLKVLSGIQIQDFSKIDRQMVEKLSGYQQIRGKLREIMRSVEYPE
                     GYFYVERTLALLFGLVAQLAPKAGLPGLVLPHASKVFARGFAKPSPAANPEVPPAAGA
                     PPTR"
     gene            3093..4556
                     /locus_tag="VFQ61_02330"
     CDS             3093..4556
                     /locus_tag="VFQ61_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953309.1"
                     /translation="MKLGRLPWFGALGLICCQTSVERPASTQHAVAPPHVGASGAVSE
                     CDYTVRVPAESPGAHFVLDVQVHCRGRAISGLRAKDELTAENVVVLDSRGQPIARSSS
                     SFLLPAPRQVAGFSYRVDLDRLAHSARNTALDYAMRRDSAWLAPASSFLLHPLPLATD
                     TRVRIHVETKGHAKFASGLLQRGDDYSAFAHELPVASYAALGEFDRYSAHVGASELEI
                     AVLGSRYALSPERLRDYVLDGARLVADFYGRFPVPHVFVSVVSVPEVDSIVFGRLLPE
                     SGPGIVLLVGEKAGEAALERDWVLVHELLHLGVPSFDNEGKWFDEGLATYFEPLIQAR
                     AGRRSSQAAFSEFAREMPRGLGALTQHGLETAKTSGGIYWGGALFCLIADVEIRRASG
                     GRVGLEAGLRAILEHGGNASEVWDLQDALKVADSAFPHPVLLPLKQRFADGTSAFDLA
                     ELWNALGVELRGPDVTFDDSAPLARVRQTLTEGASSR"
     gene            4569..6128
                     /locus_tag="VFQ61_02335"
     CDS             4569..6128
                     /locus_tag="VFQ61_02335"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HET9953310.1"
                     /translation="MTRTTSHDNTAEPESSGFAFGRPFWRTPADALLRTVVLRGVAFV
                     YLVAFAILLQQGPGLIGSRGILPAEQFLSQVAARLGSRGAGFARLPSVFWWGSSDIAL
                     RAGAYVGAGAALLVLCGIEHVSLFALLWILYLSFVHIGQIFWGYGWEILLCEAGFLAI
                     FLAPARAISCLSPRSAASPTVIWLFRWLALRLMLGAGLIKIRGDECWRDLSCLLYHYE
                     TQPNPGPLSPLFHGLPAVVQKASVLFNHFVELVVPFGLLGPARVRRIAGALVIVFQTT
                     LILSGNLSFLNWLTIVVTLACFDDAALSRIFPKALRTRAHAAQRADVSKPRWVASVGL
                     LVIVALLSINPILNMLSPRQSMNTSFDPFELVNTYGAFGSVSRERYEVIVEGSTGNDL
                     NTPIYREYEFNCKPGDPERSPCLITPYHYRLDWQMWFLSFDPERQPPWFLSFVHQLLR
                     ANPAVLGLLRSDPFAGRRPKFVRALLYRYRFTRSGESGTWRRELVGEYMRPVSLEDPE
                     FLGVLQLYGLD"
     gene            complement(6143..6526)
                     /locus_tag="VFQ61_02340"
     CDS             complement(6143..6526)
                     /locus_tag="VFQ61_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953311.1"
                     /translation="MNTSGTMLRLCVLTLSVLWGSPNSGLAALSSARAVVTQGAPSLE
                     VTSASERGHAAQKSLSDDAIRDILIRDSIASYSGNCPCPYSTARNGSRCGKRSAWNRA
                     GGAQPLCFRTDVSAEMVQRYRESNR"
     gene            6671..6991
                     /locus_tag="VFQ61_02345"
     CDS             6671..6991
                     /locus_tag="VFQ61_02345"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9953312.1"
                     /translation="MPPAKATTDVFHVIADPTRRAILDRLRAGPAPVNELAADFSQSR
                     PAISKHLRVLKQSRLVTEEKVGRERLYTLRPAPLQKAMAWLEGYRAFWGRNLESLKSY
                     LEDT"
     gene            6988..7761
                     /locus_tag="VFQ61_02350"
     CDS             6988..7761
                     /locus_tag="VFQ61_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9953313.1"
                     /translation="MTTKKNKAHSIADVAQGTILARIDIAAPPERVFRALTSDELTKW
                     WGSAEMYRVTEFRIDLRSGGSWRSDGVGADGTTFHVGGTVLEFEPPRKLVQTWEPSWE
                     TDAPPTTLSYLLEPIETGTRVTVRHTGFSDRVASCESHATGWVRVLGWLESYIAPQPE
                     RRFFLCRLLPSRPTFMQDMTADERTVMLAHGAYWRGKLAQGHVIAFGPVADPAGGWGV
                     GIVAVRDEAELLEFQTEDPAIQSNIGLRYETLPMVTAVY"
     gene            complement(7891..9309)
                     /locus_tag="VFQ61_02355"
     CDS             complement(7891..9309)
                     /locus_tag="VFQ61_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953314.1"
                     /translation="MAAPLVVICMQCADGDYVAPVACGGTRVASLGGQSFQGGAAGAG
                     AAMDGGINGKGGTGDEAVASSGGSPTGGASTTKEDDELGGAGGSSGGASGAAGESGSA
                     GRGGPRCPAKLPIWCLRSPDHLGSAGAPQDTLSKLRYAKALVPVENTTLNAVNARMQM
                     AGGMRCDDGVHALMFDRGRAYDLLEAWQDDESIATDINDHGVVVGWVTLVSGDPGYPF
                     VWADGVTMIIRGENLGTPIAINNRDQILIRGTTEQGDFIWYSGRLTPIGPSLVAYALD
                     NLGRVVGETRDELGGIGNGFIWEEGVVRAVSGFSSVRAINDRGQLAGLDEVGLPVLWD
                     GAQWVELGADFKMPLGVSARGLVLGQGWDSLSMVYWEGARETLYGQLSLGFVEGHGRD
                     ISTNGDIVGPAIAIRTDLNRSSNNCRDESCESFHAYDAIWRAGCFGSCCGGTQQAREY
                     HAVGGDAGVNSDVAGFGGAEPP"
     gene            10187..10540
                     /locus_tag="VFQ61_02360"
     CDS             10187..10540
                     /locus_tag="VFQ61_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953315.1"
                     /translation="MLTVGDDVAGFSAELEEGLNSIRVIGRGFWSADVARAIAPKVRE
                     LSSEHPRVTEFVFDMRDLKPMRDEGQQSFLEIISWVGHLRNPKMRILTSSPLTKLQFL
                     RIVRESGAGDWIEVT"
     gene            10568..10996
                     /locus_tag="VFQ61_02365"
     CDS             10568..10996
                     /locus_tag="VFQ61_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9953316.1"
                     /translation="MADEDEVLAAIRLFYVGIEAMISGRGIEPMLQAWHHVDWVTSKH
                     PLTDWAQGWDQVRTIWEASAKFGRHDRGGSKLLSARVHLRGDLALVAVVFESAPAWGG
                     ERLACTDVVERIGGVWKMIHHHADTGPAMAAALEKILSEA"
     gene            complement(11048..11458)
                     /gene="arr"
                     /locus_tag="VFQ61_02370"
     CDS             complement(11048..11458)
                     /gene="arr"
                     /locus_tag="VFQ61_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HET9953317.1"
                     /translation="MREVTTWTLYHGTRADLKPGDLIGLGYTPNFGNPVRATTYVYFT
                     RTLDAATWGAELASGEAPGRIYIVEPTGPIEEDPNLTDKKFRGNPTKSFRSREPLRVL
                     GEVANWRGHPAESIKAMKEGLARLAQMGVEPSDD"
     gene            complement(11450..11740)
                     /locus_tag="VFQ61_02375"
                     /pseudo
     CDS             complement(11450..11740)
                     /locus_tag="VFQ61_02375"
                     /inference="COORDINATES: protein motif:HMM:NF023542.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
     gene            11834..>12244
                     /locus_tag="VFQ61_02380"
     CDS             11834..>12244
                     /locus_tag="VFQ61_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953318.1"
                     /translation="MTINRLGFQAVGFALAIAAGPLAGCAGNDGAPEPAPGGLSSQGK
                     PELIGGEPVANDYFRTTVGIGNVCTAAKVGARLFLTAAHCVSLGRPGRHETPPEDYPP
                     NEGVSNAYLAGQPLHINWGLEARDENQGDVTIVKT"
BASE COUNT         2260 a         3952 c         3616 g         2416 t
ORIGIN      
        1 aggtgaaacg cgtaatcccg gccgtagaag catgcgcgcg gtgcgccgtg ccgaatgcgt
       61 cgttgacgta cacgtcagcg agctcggaca actgcttggc gaaagcttcg tcgttgttct
      121 cttcctcggc gtagaagcgg aggttctcca acaacaggac ggaaccgggg gcgagcgcgg
      181 cgacggcttg tttggccacg tcacccacgc agtcattcgc gaaagccacg gggctgccga
      241 gcagatcgga gagcgccttg gctacggggg ccaggctcat ctcgggaacg cgcttgccat
      301 ccgggcggcc caggtgagac atcagaatca ccttggctcc cttgcctgcc aagtacttga
      361 tggtcggcaa cgattcacgc aggcgcttgt cgctctcgac gacgccgtct ttgatcggca
      421 cgttgaagtc gacgcgaacg atgacgcgct tgccggacgg attgatgtct tcgatgaaca
      481 gcttcttctg agtagtcacg gcaaccccga tatcaggttc ccgcgcgcgc cgctatactc
      541 cccaacacgg cgccaactgc cgactccggc gacctgacgg gcgtctcacg ctcgcgcgcg
      601 ggcgaccgag attccagagg gaaacgcttg ttcaccactg cgtttgacgg cgaactccgc
      661 gggtaccgcc aggcatgccc gcgcccgacc ggcgtgtttc gctcggtaca acgccgcgtc
      721 agcagcccga gtcagatcac tgaccgcgac cgcttcgacc tgctccgtgg aagccacgcc
      781 aatgctgacg ctgatcggca ctggcccaac cggcgtctcc accggaacct gggccaccgc
      841 ggccccaacc cgctccgcca cgctcagggc cattccaccg ccacagtcag gcaacaccgc
      901 caagaactcc tcaccaccca tgcggcccaa cgaatcataa gcacgcacgc aggtgttgat
      961 gcggcgagcc gcttctttca ggacgacatc cccgacttcg tgcccgtagg tatcgttcac
     1021 tttcttgaag taatccagat cggccatcaa caccgcgagc ggaccaccct tgcgttgcga
     1081 ccgagccaac tctcgctcga gatgctcgag caccgcgcgt cggttcagga tccccgtgag
     1141 cccgtcgtgc atggcttcga aacgcagcga ctcgcgagcg gccaccagct gctgctgcaa
     1201 ttcgaccacg cgacttcccg cgcgcagccg caccttgagc tccagcgggt tgcagggctt
     1261 gattagatag tcgtctgcac ccgcatccaa acccgccacg atgtcggcct tgttgtcacg
     1321 ggccgtgagc aagaccaggt agacgtaggg ctcactcggg cctgctcgca ccgttcgaca
     1381 gactgccggc ccgtcgacct tgggcatcac ccaatccacc acggccaggt ggatctccgg
     1441 gtcggctttc aatgccgcta gggccgcctc accgtccttg accgtcgtga ccttatagcc
     1501 ccagcccgtg agccagtgct cgagcatggt tcgggcgatg ggttcatcgt cggcgagcag
     1561 gattttcatg gcgctcgagc aattcaccgg ccccgatcga ggtcccgcgt gaacagagta
     1621 gctcaccagc cgaaaacggc tggctcgatg ttcggcttaa gtcaccgcct tggtgctctt
     1681 gtcgcgggtc gttgctggtt tcgaaccctt tggtgctcta ggctcacccc cgtgcttcgc
     1741 cagcttcgag cactgctgct tttctggacg gttttcgcct catacggttt cgagtggctc
     1801 ctgtctcggg tgctctcgga ggcggtggtc cggcgacgca tggagcacgc gcatcgcaag
     1861 aacgcgcggc gcttggccga aggcttctcg cgtttgcgcg gagtgttcat caaaatgggt
     1921 caagtgctca gcgtgatggg caccttcctg cccagcgcgt acggcgaaga gctggagaag
     1981 ctgcaggaca aagtaccacc gcgtccgttc cgggaagtgg aactgaggct cacggaagcg
     2041 ttcggtgagg cggctttgag tcgcttcgga agcttcgaag agaccccgat cgcggcggct
     2101 tccctcgcac aagtccaccg ggcgaccaca cgggccggag aagtgctcgc ggtcaagatc
     2161 ctttatcccg gcatcgagca gctgattcgc cgcgatctct ccgtcctgcg ctcactcctg
     2221 ccagtggtac gaaggttgct ccccatatcg cgggtcgagc gcaacctcga tcagctcgag
     2281 gccatgctcg cacgtgagac cgactacgcc cacgagcgcg ccaacatact gcagatgaag
     2341 ggcacgttcg cgaaccgaca agacatcatc gtgcccaccg tgattgacga gctcaccgcg
     2401 ggcggcgtgc tcacgatgag cttcgagacc ggcttgaagg tcaccgatac cgctgctcaa
     2461 cgcgaacaag gcatcgatcc cgaggcagtg gcgcgcttat tggtggatgc ctatttcgtg
     2521 atgctgttcg accacggaat ctttcacgcg gatccgcatc cgggtaattt cctggtccgc
     2581 cccggcccca agctggtcgt gctggactac ggagccgtcg agaacgtcac acccgccctg
     2641 tccgacggca tgaagatggt cgtgctcgga gcgctgaccc gcaacgacga tcaaatcctc
     2701 atggggctcg agcgcatggg cttcgttgcc caaggcggcg atcgggagct cctatcgcgt
     2761 gtcggacgcg aatacctgaa ggtgctctcc ggtatccaaa tccaggactt ctccaagatc
     2821 gatcggcaga tggtcgagaa gctctctggc taccagcaaa tccgcggcaa gctgcgggag
     2881 atcatgcgca gcgtcgaata cccagagggt tacttttacg tcgagcggac gctcgcgctg
     2941 ctctttggtc tggtcgcgca gctcgcgccc aaagcgggct tgccggggct cgttctgccc
     3001 cacgcctcga aggtgttcgc gcgcggcttc gccaagcctt cgccggccgc caatcccgag
     3061 gttccgccgg ccgcgggggc gcctccaact cgatgaagct cgggcggctg ccgtggtttg
     3121 gcgcgttggg gttgatctgc tgccaaacgt ccgtcgagcg ccccgctagt acccagcacg
     3181 cagtagcgcc gccgcacgtt ggtgcgagcg gcgccgtttc cgagtgcgac tacacggtcc
     3241 gcgtgcccgc cgaatcgccg ggcgctcact tcgtgctcga tgtccaggtc cattgtcgcg
     3301 gacgcgccat ctcgggcctc cgcgcgaaag acgagctgac ggccgaaaac gtggtcgtgc
     3361 tcgactctcg aggacagcca atcgcgcgca gctcgagctc gttcttgctc cccgcgccca
     3421 ggcaagtggc gggcttttcg tatcgcgtcg atctcgaccg cctggcgcac agcgcgcgca
     3481 acactgcgct cgattacgcc atgcgacggg actcggcctg gctggctccg gcctccagct
     3541 tcttgttgca cccgctacca ctcgcaaccg acacccgcgt ccgcatccac gtcgagacga
     3601 agggtcacgc aaaattcgcg agcgggctac tgcaacgagg cgacgactac agtgccttcg
     3661 cccacgaatt gccggtcgcg agttacgccg ccctaggtga gttcgatcgc tattcggccc
     3721 acgtgggggc gagcgagctg gagattgccg tgttgggctc gcgctacgcc ttgagccccg
     3781 aacggctacg cgactacgtc ctcgacggcg cgcgtttggt tgctgatttt tacgggagat
     3841 ttccggtccc gcacgtgttc gtctcggtag tttcggtgcc cgaagtagat agcatcgtat
     3901 tcggccggct cttgcccgag agcggccctg gcatcgtcct tctcgttggc gagaaagcgg
     3961 gcgaggcggc actcgaacga gactgggtct tggtacacga gctactgcac ctaggagtcc
     4021 cttctttcga caacgaaggg aagtggttcg acgaaggcct agccacctac ttcgagcctt
     4081 tgatccaggc ccgtgccggg cgtcgaagta gccaagcggc cttttccgag ttcgcccgcg
     4141 agatgccgcg cgggttgggc gccctcacgc agcatggttt ggagaccgcc aaaacctcgg
     4201 gtgggatcta ttggggggga gcgctgttct gcctcattgc agacgtcgag atccgtcgcg
     4261 cgagcggcgg ccgagtggga ttggaagcgg gactgcgcgc cattctcgag cacggcggca
     4321 acgcctcgga agtctgggat ttgcaggatg ccttgaaggt ggccgatagt gcattccctc
     4381 accccgtgct gctgcccctg aagcaacgct ttgccgatgg cacgtctgcg ttcgatttgg
     4441 cggagctatg gaacgcgctc ggcgtcgagc tccgaggccc ggacgtcacc ttcgatgatt
     4501 cggcaccgct agctcgggtg aggcagaccc tcacagaggg cgcaagttct cgttgactct
     4561 tatcgtagat gacccgcaca accagtcacg acaacacggc agagcccgag tcgtcgggtt
     4621 ttgctttcgg aaggccgttt tggcgaacgc ctgccgacgc ccttctgcgc acggtcgtac
     4681 tgcgcggagt agccttcgtc tacctcgtcg cgttcgcaat cctactacaa caaggccccg
     4741 gtttgattgg ctcgcgtggc attctgcccg cggagcagtt cctgagccaa gtagcagccc
     4801 ggctcggatc gcgaggagcg gggttcgcca gactgccaag cgtgttctgg tggggttcga
     4861 gcgacatcgc tctgcgcgcg ggagcctatg tgggcgcggg agccgcgctg ctcgtgctgt
     4921 gcggcatcga acacgtgtcc ctgtttgcgt tgctctggat tctatatctc tctttcgttc
     4981 acatcggaca gatcttctgg ggctacggct gggagatctt attgtgtgaa gcgggattcc
     5041 tcgcgatctt tctcgctccc gctcgcgcca tatcttgttt gagcccacgc tctgccgcct
     5101 cgccgaccgt gatttggcta ttccgctggc tcgcgttgcg gctgatgctg ggcgccggac
     5161 tcatcaagat ccgtggcgac gagtgttggc gcgatctctc ctgcttactc taccattacg
     5221 agacacagcc caaccctgga cccctgagcc ccttatttca tggtctgccc gcggtggttc
     5281 aaaaagcgtc ggtgctattc aaccacttcg tcgaactcgt cgtgccgttc gggttactgg
     5341 gccccgcgcg tgtgcgtcgc atagctggcg cgctcgtcat cgtgtttcaa accaccctga
     5401 ttctaagcgg caatctctcg ttcttgaact ggctcaccat cgtcgtcacc ttggcttgtt
     5461 tcgatgacgc ggcgctgtcg cgcattttcc cgaaagcact ccgaacccgc gcccacgccg
     5521 ctcaacgcgc cgacgtatcc aaaccacggt gggtcgcgag cgtggggttg ttggtcatcg
     5581 tcgcgctgct cagcatcaac ccaatcctaa atatgctctc gccgcgacag agcatgaaca
     5641 cgtctttcga ccccttcgaa ctcgtcaaca cctacggtgc gttcggcagc gtcagccgag
     5701 agcgctacga agtcatcgtc gagggcagca ccggcaacga cctcaacact ccaatctatc
     5761 gagagtacga attcaattgc aaaccgggag acccagagcg ctcgccctgc ctaattacgc
     5821 cttatcatta tcggctagac tggcagatgt ggttcttgag cttcgatccc gagcggcaac
     5881 ccccttggtt tctaagcttc gtgcatcaac tgctgcgcgc gaacccggcc gtcttgggcc
     5941 tgctccggag cgatcccttc gccgggcgaa gaccgaaatt cgtgcgcgca ctattgtatc
     6001 gataccgatt cacgcgctcc ggcgagagtg ggacttggcg tcgcgaactc gtaggtgagt
     6061 acatgaggcc cgtgagcttg gaggatcccg agttcctcgg agtactgcag ttgtacgggc
     6121 tcgactgaag cggcaccggt cgctagcggt tcgactctcg atatcgctgc accatctctg
     6181 cggagacatc cgtccggaaa cagagcggct gtgctcctcc cgctcgattc caggcactac
     6241 gctttccaca ccgagacccg ttccgagccg tactgtaagg acaagggcaa ttgccagagt
     6301 agctcgcgat tgagtcccgg atcagaatat cccgaatcgc atcgtcgctc aggctcttct
     6361 gcgcggcgtg gcccctctcg ctagccgagg tcacctcgag gcttggcgca ccctgcgtga
     6421 caacggcgcg agccgagctc aaggctgcca gtcccgagtt cgggcttccc cacagcacgc
     6481 tcaacgtgag cacgcacagg cgcagcatcg ttccagaagt attcacggcc agcagccaaa
     6541 catcatgaga agattttcag gccacacccg actcgccgcg cctatcctca actacatcga
     6601 ccaccctcct ccctactaac gcttgaccgg ccgcgagccg cgcgatacag gtaacccatc
     6661 ggttacctgt atgccgcctg ccaaagccac cacggacgtg ttccatgtca tcgccgatcc
     6721 gactcgtcgc gcgattctcg atcggctgcg tgccgggccg gcgccggtga atgagctggc
     6781 ggctgacttc tctcagtcgc gaccggctat ttccaagcat ctacgggtgc tcaaacagtc
     6841 ccggcttgtc acggaagaga aggtcgggcg cgagcgtctg tacacgctcc ggccggctcc
     6901 gttgcaaaag gccatggcct ggctcgaggg ctatcgagct ttctggggtc gcaatctcga
     6961 gtcgctgaag agttacctag aggatacatg acaacgaaga agaacaaggc tcactcgatc
     7021 gccgatgtcg ctcagggcac gatcttggcc cgcatcgaca tcgccgcacc gccggagcgt
     7081 gtatttcgcg cgctcacgag cgacgagctc acgaagtggt gggggtcggc cgagatgtac
     7141 cgggtcacgg agttcaggat cgatcttcgc tcgggagggt cgtggcgaag cgacggcgtg
     7201 ggagccgacg gcacgacgtt ccatgtcgga ggcaccgttc tagagttcga gccgcctcga
     7261 aagctcgttc agacctggga gccgtcttgg gagaccgatg cgccgccaac gacgctatcc
     7321 tatcttctcg aacccatcga gaccggaaca cgcgttacag tccgccacac cggctttagc
     7381 gatcgggtcg cgtcgtgcga aagccacgca acgggctggg tacgcgtcct gggatggctc
     7441 gagagctaca tcgctcctca gcccgagcgg cgcttcttcc tctgtcgcct gctgccctct
     7501 cgcccaacat tcatgcaaga catgaccgcc gacgagcgca cggtgatgct ggcgcacggc
     7561 gcgtactggc gcggcaagct cgcccagggc cacgtgattg cattcggccc cgtcgctgac
     7621 cctgcaggcg gatggggcgt cggcatcgtt gcggtgcgtg acgaggccga gctcctcgag
     7681 ttccaaaccg aagatcccgc gatccaatcc aacatcggac ttcggtatga gaccttgccg
     7741 atggtaacgg cggtgtactg agttacccaa gctgaaccga cagaacgcga atagcggcgc
     7801 ttcaaccatc gcgggcacga ccccattcga ttcatcctgg atttcgcgag cacttgccgc
     7861 cgcaggacta ggatgctgcg aaatccaggt tcaaggcggc tcggcaccac caaagccggc
     7921 aacatccgag ttcacccccg catcgccccc tacggcgtga tactcgcggg cttgttgcgt
     7981 gccgccacag caggagccga aacagccggc gcgccagatc gcatcatatg catgaaagct
     8041 ctcgcaactc tcgtcacgac aattgttcga gctgcgattc aagtcagtgc gaatcgcaat
     8101 ggctggccca acgatgtcac cgttggtgct gatgtcgcga ccgtggccct ccacgaagcc
     8161 caaagagagt tggccataga gtgtctcccg cgccccctcc caataaacca tcgacaggct
     8221 gtcccaacct tggccaagca ccagcccgcg tgcactcact ccaagtggca tcttgaagtc
     8281 tgcccctagc tccacccact gggctccgtc ccagagcacc ggcaaaccaa cttcgtcgag
     8341 cccagccagc tgtccgcggt cgttgatcgc tcgaacgctg ctgaagcctg agacggcccg
     8401 gacaacgcct tcctcccaga tgaatccgtt gccgataccg cccaactcgt ccctcgtctc
     8461 gccaaccaca cgcccgagat tatcgagcgc gtaggcaact agactgggac cgatcggcgt
     8521 aagcctgccc gagtaccaga tgaagtcacc ttgctccgta gtgccgcgaa tcaggatctg
     8581 gtcgcgattg ttgatcgcga ttggcgtgcc aagattctct cccctgataa tcatggtcac
     8641 gccatcggcc cagacgaacg gatagcccgg gtcgccactg acgagagtca cccatccgac
     8701 tactactccg tggtcattga tgtccgttgc aatgctctcg tcatcttgcc atgcctcgag
     8761 caaatcatag gctctgcctc tatcgaacat cagcgcgtga actccgtcat cacagcgcat
     8821 gccccccgcc atctgcatgc gcgcattcac cgcgtttagc gtcgtattct caaccggcac
     8881 caaggccttc gcatagcgta gcttactcag tgtgtcctgc ggtgcgccgg cagagcctaa
     8941 atgatcaggg cttctcagac accaaatagg caattttgcc gggcaacgcg ggccgcctct
     9001 ccccgcgctc cctgactcac cggcggcacc tgatgcgcca cctgatgatc cgcccgctcc
     9061 gcccaattca tcgtcttctt tcgtggtgga ggcgcctccc gtcggcgagc caccgctgct
     9121 cgcgaccgct tcatcgccag tacccccttt cccgttaatt cccccgtcca tggccgcgcc
     9181 ggcaccggcc gcgccgcctt gaaacgattg ccctcccaaa gaggccacac gagtcccacc
     9241 gcacgctacg ggcgccacgt agtccccgtc cgcgcactgc atgcaaatga ccaccagcgg
     9301 cgctgccaat ttagatccgc gagctcgatg tgcttccctc gtcgccatct ggcctccctc
     9361 gccgaactct agagcaacca caaatccgtt tgctccaagc ctcccgcgta agcggcttgg
     9421 agctatctcc gaccgccagt aaaggagtca cgccttggtg cagcgctact cgaaccggat
     9481 cctgctttag cacgagaaga ttcgtgcgga tatctcggtg aggtcagcac ggcatacggg
     9541 tgacgcgcct caggcacttg aatctatcga gatacggttt ggtgacccgt attcgtgtcc
     9601 tactcagttc tgccctttcg gcggtctata cggcagcatt ggcatccacg gaggccaaac
     9661 aggttcattg tggcgcattc agacgaactc caccgattca agtgagatga aggcgcgcgg
     9721 ggggagtggc gcttgccgga tatccagctc gagtcggcaa tgttgaagga gctgttttgg
     9781 gcggcggcaa agatgacgtg cgcgacgcgc gtcatcttcc gtcgcgagcg cagacgggtt
     9841 gcaacaagct ccccgcgtcc accgcgccca ccgcccgcaa taacgtctga tgttgcggag
     9901 cttgggcccc aatcgtcccc gagctctctt ctctcagcac ttcgctgagg aacactatga
     9961 ttctccttgc tagtcttcct ggaagctcga gaacggtgtc ccggaagggc ccgtgcgtag
    10021 cagactgacc tacacccagt ttggtggggc gcaggaccat gagcgtttcc aaggtagcga
    10081 ctcgaagttg gcggtgagaa agttggcgac gagctagacc gagccaaccc atgaaaatct
    10141 tgacttttta cgcctgcggg agtcagggtc gtggggattg gcaatcatgt taacggtcgg
    10201 tgacgatgtg gcgggtttct ccgcggagct tgaggaagga ctcaactcga ttcgagtcat
    10261 cggccggggc ttttggagcg ccgacgtagc acgggcgatc gcacccaagg ttcgagagct
    10321 aagtagtgag catcctcggg tcactgagtt cgtgttcgat atgcgcgacc tgaagcccat
    10381 gcgtgacgaa gggcagcaaa gctttctgga aatcatttcc tgggtcgggc atcttcggaa
    10441 tccgaagatg cgaatcttga catcgagccc gctcaccaag ctccaatttc tgcgcatcgt
    10501 tcgggagagt ggagccggcg attggatcga ggtaacttga ccccccacgc tcggaggatc
    10561 agccacgatg gcagatgagg atgaagtgct cgctgcgatt cgcctgttct atgtcggcat
    10621 cgaagcgatg attagcgggc gcggaataga acctatgttg caggcgtggc accacgtgga
    10681 ttgggtcacg agcaagcatc cattgactga ctgggctcag ggttgggacc aggtgcgaac
    10741 gatctgggag gcttcggcca agttcggccg tcacgaccgg ggtggctcca agctcctgag
    10801 cgcgcgcgtg catctgagag gggacttggc gctcgtcgcc gtggtcttcg agtctgcgcc
    10861 ggcgtggggc ggagaacgct tggcatgcac cgacgtcgtg gaacgcatcg gcggagtttg
    10921 gaagatgatt caccaccacg ccgatactgg gcctgcgatg gcagccgcgc tcgagaagat
    10981 cttgagcgag gcataagcac gggcaccgct cgcagcctct ctcgtctcgg agaggctgtc
    11041 catctcgtta gtcgtccgac ggctccacgc ccatctgggc gagtcgcgcc aatccttctt
    11101 tcatggcttt tatcgactca gccgggtgtc cgcgccagtt cgctacctca ccaagaactc
    11161 gcaggggctc gcgcgagcgg aatgacttgg tcggatttcc acgaaatttc ttgtcggtca
    11221 aatttgggtc ctcctcgatc gggccggtgg gttcgacgat atagattctg cccggagcct
    11281 caccgctggc taactccgcg ccccacgttg cggcatctag ggtgcgggtg aaatagacgt
    11341 aagtggtggc cctgaccgga tttccaaagt tcggtgtgta acccagccca attagatcgc
    11401 cgggcttcag atccgcgcgg gtcccgtggt acagcgtcca cgtcgtaact tcgcgcacga
    11461 cccgcagcgg ctcggtcgag tagcaggaca tcgccggatg ccccaaggcc ttccgtctgg
    11521 aatcgcctgc atcggcaact tcgccggcag gctccacgac ataaactttg ggtgacccca
    11581 ccccgttggc gatttcggcc tcccaaatcg cctcatctat attccgagtc agcagaacat
    11641 actcgaactt ctcgtcgagt ccactgccta gatggcggct cggctcaatc gagtccccct
    11701 cgttcaagtc agcccgggta ccgtagtaat atagactcat gggcaggccc tttcggaaaa
    11761 gcagcgagct cgtcacccga tccttcagcg atcggtactg atgggaggta agagctgttt
    11821 tcggagaggt atcatgacga tcaatcgact tggttttcaa gccgtgggct tcgccctggc
    11881 catcgcagca ggtccactcg ccggttgtgc cggaaatgac ggagcgcccg agcccgcacc
    11941 gggcggcctg agctcccaag gtaaacccga gctgattggc ggcgagccgg tcgcgaacga
    12001 ttactttcga acaacggtgg gcatcgggaa cgtttgcacc gccgccaagg taggggcacg
    12061 gctctttttg acggcggcgc actgcgtgtc tctcgggcgc ccgggtcgcc acgaaacgcc
    12121 gcccgaagac taccctccca acgagggcgt cagcaacgct tacctagcag ggcaaccact
    12181 gcacatcaat tggggactcg aggcgcggga cgagaaccag ggtgacgtca cgatcgtgaa
    12241 gacc
//
LOCUS       DASTKU010000071         8708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_13789,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000071 DASTKU010000000
VERSION     DASTKU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13789"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFQ61_02385"
     CDS             complement(<1..329)
                     /locus_tag="VFQ61_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9953319.1"
                     /translation="MIGIGVIGYGYWGPNLVRNFSQADGSRVVAVCDPRADRRKHAVS
                     TYPAITAVETIPELLANPAVDAVAIATPVSTHFDLAMQALQAGKHVMVEKPMTSNSDQ
                     ARRLIDEA"
     gene            complement(711..1316)
                     /locus_tag="VFQ61_02390"
     CDS             complement(711..1316)
                     /locus_tag="VFQ61_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HET9953320.1"
                     /translation="MIKIHPEAEISPEAEIGDGSMVWRNVQIRERAKLGTKCIVGQSA
                     YIDCDVHIGNNVKIQNGAALYDGLTVEDGVFVGPHVVFTNDKVPRGINPDGTLKSAAD
                     WVVGRTRVCYGAAIGASSVIVTGVTIGRWAMVGSGAVVTKDVPDHALVLGNPARVVGY
                     VSAKGVRCRTQAEAEALTRTEQAGGQGVLASEQSSSHASKT"
     gene            complement(1382..3226)
                     /locus_tag="VFQ61_02395"
     CDS             complement(1382..3226)
                     /locus_tag="VFQ61_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953321.1"
                     /translation="MEKEVVETAATAGTPPVAQPRTTRGIWLLVGVIGCWLLLAWLKL
                     RAERPVYDWMSWRQADTQAIARHFLEPGSNWLWPRIDWGGAGPGYVETEFQLYTGLVS
                     LLLRAFGENEWAGQALSLAISATTAGLIARWLLRRLGLLPALFGVVAWLSVRSVLLLS
                     TAVMPDGLALLLYVSAWFGFCHYLEADHSDGRLENYSSAGPLASLAKPYWGLVAFGVC
                     GALAMLVKPTTAHLGISSAVLVILLKPRELAKPTLWLVWLGMLGTLGAYLFHAKQLYA
                     EYGNTFGILSGGDSKLPKPEHLLMPRLHALAFGRFVNFGLGPAGALAVLALGVTRLRQ
                     RAFWSPGSWASDEARWLALLVGNLAIVPLSFRYLANGAGAHYVAPASLLGASAVAQWV
                     ARLRRGTNTPPRSDRTEPSSPKPRTPLALLMEKLRRLAVAMLGALLLLQLALGIRVRL
                     MSNRDAQSVALAELGEAVASQASSTSKQLLVVRTGEPEFDAFYRTPNNFEDPRVFYAA
                     RMRGFAIGADADTPARLEAARKQGARFFVDSALEPRGPRTEAWLRQHARPLAHSSSHP
                     SLDPNLTTSRSETERAPNDQSPNSGHRQPELPVTSLLDVKARIYRLMP"
     gene            complement(3536..4882)
                     /locus_tag="VFQ61_02400"
     CDS             complement(3536..4882)
                     /locus_tag="VFQ61_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953322.1"
                     /translation="MKSSNHRNGAASSTATDEDLEISVLEGPALPDDVDSLFDASEDD
                     VTVARPRSELLSQSDELAKHRELLAAKRAQQAETAMNNGSAANPPSSANPNNAVDSNS
                     AAPDSVSPDSVPPDSAVPISVMPIESQDFIRDRPSDEPVTMRRPDESRTATTRRVPRM
                     DRTPHPPRRANPRRSQSEPEIRELPSDAPTAADAPHPVAAEAPRNHSLKWAGVGLLLV
                     IGLIVGKRLVDAQHVAAPTAAKPPAVEPAEPKPATNELVSAPTLPIAEPSASLQSAAS
                     ASNASEISGDASPPTAGASDPKSALNTPPANPGGISGPAGARANSQTQPRNGTRSVSS
                     LAPNKASTPGNSPTKLAPAPDEGASSAFDAAAANAALTGTAAKAGACRQADDPSGVAV
                     VTITFAPSGRVTSATISGPPFAGTKTGSCIASTLRSTRVPAYEGEFITVRKTVVID"
     gene            6031..7728
                     /locus_tag="VFQ61_02405"
     CDS             6031..7728
                     /locus_tag="VFQ61_02405"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9953323.1"
                     /translation="MPSVSPHQPQAPEAVLRSGVLRELVWSRPHSIPSEISSETSSEQ
                     APPEPEAFLERNADSASDGALDAELPQQNLLVTVAAQKSPESSGHALVRYEAGVIIGG
                     KYELSELLGEGGMGWVFGAQNLTLGVPVAVKLMRSDLARETHGLTERMLQEAQAAASL
                     GHPSIIRVLDFGFTECADPYIVMERLEGESLSRRLHRRGPIRAARAVEVLLPIIDALA
                     EAHDAEIVHRDIKPENIFLARRATGASASGRGVQPKLLDFGIAKWTSRASARLTREGT
                     ILGSPAYMAPEQVRGEPDIDGRVDQWSVCVVIYETITGRRPFEAENYHAEMWSILTSE
                     PKPLSAHDVHEPALWRILSRGLSKERANRYASLHDLHDALENWLRMRRTGQGATTSTD
                     SAPESTAAAAPSSAANATEPRKISGGVEGLVLGLFASRSGRLNESVAGQALAEQEVPL
                     ARVRNGRNSIRWPWWLAGVFVLIVSGASVWLWSSFFGEPTASDDAEEPESIPAAPRRA
                     RHARATRPLAPLPTTGPQAPLLYLEPRDGRARGANPPRESPKPGSPSMHDVKPTEDL"
     gene            complement(7693..>8708)
                     /locus_tag="VFQ61_02410"
     CDS             complement(7693..>8708)
                     /locus_tag="VFQ61_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET9953324.1"
                     /translation="MDVHTVVVFTIAVALLFDLINGFHDAANSIATIVSTRLLTPPVA
                     VAWAAFFNFVAFFIFHQGVAKTVSGLVKVNAADPGFVWVVLAGLLGAIVWNMFTWWLA
                     LPSSSSHAIMGGFSGAGFAYGGAKVLNIPKLVLTSEFIVISPTMGFVLGGLLLVALSW
                     VLRRVRPQKVNRSLRFVRLLSAAGYSIGHGGNDAQKTMGIILAVLVAGGYIDPNLEQT
                     PTWVALSCYAAMALGTALGGWRIIKTLGSRLTKLKSHSAFCAEISGALTLFFATHMKI
                     PVSTTHTIVGAIVGVGSTNRMRGIRWGLASNIVWGWVFTIPAAASTSALCLQIFRWLD
                     VVH"
BASE COUNT         1580 a         2704 c         2854 g         1570 t
ORIGIN      
        1 gcctcgtcaa tcaggcggcg cgcctgatcc gagttcgagg tcatgggctt ctcgaccatg
       61 acgtgcttgc cggcctggag cgcctgcatc gcgaggtcga agtgcgtcga taccggggtc
      121 gcgatcgcca ccgcgtccac cgcggggttg gcgagcagct cgggtatggt ctccacggcg
      181 gtgatggccg ggtaagtcga tacagcgtgc tttctacggt ccgcgcgggg atcacagacc
      241 gcaaccacgc gcgagccgtc ggcttgcgag aagttgcgga cgagattggg cccccagtag
      301 ccgtagccga tgacaccaat tccaatcacg atgcgtgcct ttcttgaatt gtctcgaccg
      361 agtcgttcga agtgaatcgt tcggagtgct gagccgcccg ggactaacgc tgccgtgagc
      421 tccgagtccg ctcgagtgcc ggttatcgcg cggcccctcg aatcaggcgg cgctcaatcg
      481 gtggcctcct caatggaggg gcctgcggcg ctattcccga gaccgcgaac ttcgctccga
      541 agcgcgcggg ccttgcgcag acgttacacg gggcttgcac tgtttttcga gaaagtgcct
      601 cgggagtgga cctttgccgc ggtgggtacg ttaacacctg tgtgccgcta aaagcgaccc
      661 cgaacctacc gcgccacaac actactattc aggccttcag acccccgcag tcaggtcttg
      721 ctggcgtggg aggacgattg ttcagaggcg agcacaccct gaccgcccgc ttgctccgtg
      781 cgcgtgaggg cttcggcttc cgcctgggtg cgacatcgca cccctttggc gctgacgtag
      841 cccaccactc gagccgggtt gccgagcacc aaggcatggt ccggcacgtc tttcgtgacc
      901 accgctccag agccgaccat cgcccaacgc ccgatggtta ccccggtcac gatcacgctc
      961 gacgcgccga tcgccgcgcc gtagcagacg cgcgtgcggc caacgaccca atcggcggct
     1021 gacttcaacg tgccatccgg gttgatgcca cgaggcacct tatcgttggt gaagaccacg
     1081 tgtggaccga cgaacacccc gtcttcgacg gtcaacccat cgtagagagc ggcgccgttc
     1141 tggatcttca cgttgttgcc gatgtgcacg tcgcaatcga tatacgcaga ctgccctacg
     1201 atgcacttgg tgccgagctt cgcccgctcg cggatctgga cgttgcgcca gaccatcgaa
     1261 ccatccccaa tctctgcttc tggagagatc tccgcttcgg ggtgtatttt tatcattggt
     1321 tctccgacaa atcgcaaaga cgctcaatat accgaaggct tttgacgacg gaatagcaaa
     1381 ttcacggcat tagccggtaa atgcgggctt tgacgtcgag caggctcgtg accgggagct
     1441 cgggctggcg gtggcccgaa ttgggagact gatcgttcgg ggcgcgctcg gtttcggacc
     1501 ggctcgtcgt gaggttcggg tccaggctcg ggtgcgagct cgagtgcgcg agtggccgag
     1561 catgctgccg cagccaagcc tccgtgcgcg gcccccgcgg ctcgagcgcg gaatcgacga
     1621 agaaacgtgc cccctgtttt ctcgctgctt cgagccgcgc gggagtatcg gcgtcggcac
     1681 cgatcgcgaa gccgcgcatg cgggcggcat agaacacccg cgggtcttcg aagttgtttg
     1741 gcgttcgata gaatgcgtcg aactctggct cgcccgtgcg cacgacaaga agctgcttgg
     1801 aagtggagga tgcctggctg gccacggcct caccgagctc agctagcgcg acgctctgag
     1861 catcccgatt ggacatcaaa cgcacgcgaa tgcctagtgc gagttggagg agcaacagcg
     1921 cgcccagcat tgcgactgcc aggcgtctga gcttttccat tagtaatgcc agcggggtcc
     1981 ttggtttcgg actcgacggc tcggttctgt cgcttcgcgg cggcgtgttc gtgccgcgac
     2041 gaaggcgcgc cacccactgg gcaaccgcgc tcgcgccgag caagctggcg ggcgcgacgt
     2101 agtgtgcgcc ggcgccgttc gcgagatagc gaaaggagag gggtacgatc gcgaggttgc
     2161 caaccagcag cgcgagccag cgagcctcgt ccgaggccca gcttccgggg gaccagaaag
     2221 cgcgctgacg aagtcgggtc acgccgagcg caagaacggc cagtgccccc gcgggcccga
     2281 gcccgaaatt gacgaagcgc ccgaaagcca gagcatgaag cctcggcatc aacaggtgtt
     2341 ctggcttggg taacttggag tctccgccgg atagaatgcc gaaggtgttt ccgtactcgg
     2401 cgtagagctg cttggcatga aacaagtagg cccccagcgt gcccagcatt cccaaccaaa
     2461 ccagccagag agtgggcttc gcgagttcgc gcggcttcaa cagaatgacc aagaccgcgc
     2521 tcgagattcc gaggtgggcg gtggttggct tcaccaacat cgcgagtgcg ccgcacacac
     2581 cgaacgcaac caaaccccag tacggcttcg ccagtgaggc gaggggaccg gccgaagaat
     2641 aattctcgag ccgcccatca ctgtggtcgg cctctagata gtggcagaag ccaaaccagg
     2701 ccgagacgta aagtaacaag gccaagccgt ctggcatcac cgcggtcgag agcagaagta
     2761 cgctacggac cgagagccag gccacgaccc cgaacagcgc cggcagtaag cctaagcgtc
     2821 gcagtaacca gcgcgcaatg agcccggcgg tcgtggcgct gatcgcgaga gacaaggctt
     2881 gcccagccca ctcgttttcg ccgaaagcgc ggagcagtaa cgagactagc ccggtgtaaa
     2941 gctgaaactc ggtttcgacg taccccggcc ccgctccgcc ccaatcgata cgcggccaca
     3001 gccagttgga tcccggttcg aggaagtggc gtgcgatggc ttgcgtatcg gcctgacgcc
     3061 aagacatcca atcgtaaacg gggcgctcag ctcggagctt caaccaagcg agcaacagcc
     3121 aacacccgat cacgccaacc aacagccaga tcccgcgagt ggtccggggc tgagcaaccg
     3181 gaggcgtgcc ggccgtagct gcagtctcga cgacctcttt ttccaaacca ggttcccttc
     3241 gcagatccaa ttcgaacgcc gccacgtcgg ggcgcgcggc tggtcccaat aggatcgcaa
     3301 cctggtctta acctatatcg acgggagcca aggcaagaaa tcgccgcctg cggggacgag
     3361 tcttggaacg ctcgttcagt cgcacgaggc taggtgtcgc cgccgtgtcg atcgagcacg
     3421 gtctgactcc aaccccgggg agggcgaaag ctccggggga gggcaaagct aacgagcgaa
     3481 ttccagccgg gaggaggcgg tccgtggctg aggggagtgc gggaggaatg ggtgatcagt
     3541 cgatcacgac cgtctttcga accgtaatga actccccttc gtatgcgggg acgcgcgtgc
     3601 ttcgcagcgt ggacgcaatg cagcttccag ttttggtgcc tgcgaaggga ggaccggaga
     3661 tcgttgccga cgtgacacga cccgaaggcg cgaacgtaat cgtgacgacg gccaccccgc
     3721 ttgggtcatc ggcttgtcgg caggctcccg cttttgcggc ggtgcccgtt agcgctgcgt
     3781 tggctgccgc ggcgtcgaag gccgaagagg ccccctcgtc gggcgccggc gccaacttgg
     3841 tgggcgaatt gccgggtgtc gaggccttgt tcggcgccaa agaggagacc gagcgcgttc
     3901 cgtttcgcgg ctgagtctgg ctattggctc gtgcgccggc cgggccgctg atgccgccgg
     3961 gattggccgg tggcgtgttc agcgcggatt tcggatcact tgcgccagcc gtgggcggac
     4021 tagcgtcgcc cgaaatctcc gacgcgttgg aagcgctagc ggcactttgc agggaagcgg
     4081 atggttccgc gatcgggagc gtcggggcgg acaccagctc gttggtggcg ggcttcggtt
     4141 cggccggttc cacggctggc ggcttggccg cggtcggcgc cgcaacgtgc tgcgcgtcga
     4201 ccaagcgctt gcccacgatc aagccaatca cgagcaagag tccaacgccc gcccacttga
     4261 gggagtgatt gcgcggcgct tccgccgcaa ccgggtgtgg cgcatcggcg gccgtcggcg
     4321 catcgctcgg gagctcacgg atttcgggct cggactggga ccgacgcggg tttgcgcgcc
     4381 gcggggggtg aggcgtccga tccatccggg gcacgcgccg cgtggttgcg gtacggctct
     4441 cgtctggtct gcgcatcgtg accggttcgt cgctcgggcg atcccgaatg aaatcctgcg
     4501 actcgatcgg catcaccgaa atgggaaccg cagaatccgg aggaacggag tcgggggaaa
     4561 cagaatccgg agcagcagag ttcgaatcaa cagcgttgtt cgggttcgcc gaggacggcg
     4621 ggttcgccgc gctgccgttg ttcatcgcgg tctccgcctg ttgagcccgc ttcgcggcga
     4681 gcaactcgcg atgcttcgcg agttcgtcac tttggctcag cagctccgag cgcggccggg
     4741 caaccgtcac gtcgtcttcg ctggcatcga atagcgagtc gacgtcatct ggcagtgccg
     4801 gcccctcgag gacggagatt tccaggtcct cgtcagttgc ggtggagctt gcggcgccat
     4861 ttcggtgatt gctcgacttc atgcggcctc ccagggtcga agtgctcgcg tctttgacgg
     4921 caacaggggc tccacatttt cggcacgggc tcagcgctgc cttcgctgaa tcctcggcgg
     4981 tctcatattt cgcgccgcat tgggtgcatt cgactgaaca catgctgggt tgtcctggtg
     5041 ctgcgcgcct cgagctgacg cgggtgaata gccggttgct tccgagcctc gctaggcccc
     5101 gggcccttcg cggcttcccg caaaaatggc cctgctaaac cacgggtgca agcccagaag
     5161 tgaacttggt gcaagtgggg agctcttggg acatcgaacc gtattgagtg cctccgagta
     5221 acagaccaag agctccccgc gaatcgacgc ggggcttcgg gcggctcgag ccgaggctcc
     5281 cttcttacgg cgagcgtgtt gcgcttcgca gggtgtcgcg ttcacggcgt cgcggcgcac
     5341 tggggcttgc gtggcccgtg cccttggttt tcacgggcga ttggctggcg ccagcaccgg
     5401 tgccccagca ctcggcgggg tgcgttctac ttatgctgct cgggctctca tgcagtccag
     5461 cgactcgagc tgcgcctctc gagacgggat tgcagccggc gacgtagatg cccttgctgt
     5521 ggttgccgca atgcttctcg accttgcggc gttgaaaccc cgcgacggag tgaatgccgc
     5581 ggcggctgac ccaaacgttg tgagttgcac ttatgcagtt gactcgcgat ctggcggaat
     5641 ccggcgttgc tgcgaacggc ccgcgatttt ggggagttct ccgcgatttt ggggagttct
     5701 ccaggggctg cgtcccgcgt cactctcgag accgacagcg atccgagcgt ggtcggtccg
     5761 cgatctcagc acctcctagg ctgctctcga ggctctcgtt agactctcga gatgccgcgt
     5821 tctgccattg accccaggtc gactcgagcc ccggctctta cttaagtacg caagggcgat
     5881 acgctcggcc tagtgcgccc aatttgataa gacctcggtt ggagccgata cgctgagagc
     5941 gtagtcgaga gcaggttggc tcagattgct gcgcccggtt gcccggtgcg agtttccccg
     6001 ggtgtgggtg ccacaccgcg aaggatcagc ttgccctcgg tctcgccaca tcaacctcag
     6061 gcaccagagg ccgtattgcg gtccggagtt ctgagagaat tggtctggtc gcgcccgcac
     6121 tctatccctt ctgagatctc ttcggagacc tcttcggagc aggctccccc tgagccagag
     6181 gctttcctcg agcgaaacgc cgattcggcg tccgacggcg cgctcgacgc agagcttcct
     6241 cagcagaact tgctcgtgac cgtagcggcc cagaaatctc cggaatcgtc cggacacgcg
     6301 ctcgtgcgct acgaagccgg ggtcatcatc gggggcaagt acgagctctc ggagctgctc
     6361 ggcgagggcg gcatgggttg ggttttcggc gctcagaatc tcactttggg cgtgccggtt
     6421 gcagtgaagc tgatgcgctc ggacttggcg cgcgaaactc atggcctcac ggagcgaatg
     6481 ctgcaagagg ctcaggctgc cgcgagcctg ggacaccctt ctatcattcg agtgctcgac
     6541 tttggtttca cggagtgtgc ggacccgtac atcgtcatgg agcggctcga aggagagagc
     6601 ctgtcgcggc ggttgcatcg gcgcggtccg attcgagccg cgcgtgcggt cgaggtcttg
     6661 cttccgatta tcgacgcatt ggcggaggcg catgacgccg agatcgtgca tcgcgacatc
     6721 aaacccgaga atattttcct ggctcggcgc gcaaccggtg cgagcgcgag cggtcgaggc
     6781 gttcagccca agctgctcga ctttgggatt gccaagtgga cgagtcgggc gtcggctcgt
     6841 ttgacccgcg aaggcacgat cttggggagc cctgcgtata tggcgcccga gcaggtgcgt
     6901 ggagagccag atatcgatgg gcgggtggac caatggtccg tgtgcgtcgt gatctacgaa
     6961 actattacgg ggcggcgccc gttcgaggcc gagaactatc acgccgagat gtggagcatc
     7021 ctgacttcgg agccgaagcc gctatccgcg catgacgttc acgagcccgc tctgtggcgg
     7081 atcctgtcgc gcggcttgtc caaggagcgc gcgaatcggt acgcttctct gcacgacctg
     7141 catgacgcgc tcgaaaattg gctgcgcatg cggcggacag gtcaaggtgc cacgacgtcg
     7201 actgactctg ctccggagtc gaccgccgct gctgccccga gctccgccgc gaatgctacc
     7261 gagccgcgca agatcagcgg aggagtggaa ggtttggtgc tcgggttgtt tgcgagtcgt
     7321 tccgggcgcc tgaatgagag cgttgcgggc caagctctag ccgagcaaga agtgccactg
     7381 gctcgggttc ggaacggacg caactcgata cggtggccct ggtggcttgc gggcgtcttt
     7441 gttctaatcg tgtctggcgc aagcgtttgg ctttggagct cgttctttgg tgagccaacg
     7501 gcaagtgacg acgccgagga gccggagagc atacccgccg cgcctcggcg cgcccgacac
     7561 gcccgcgcta ccaggcctct cgctccctta cccacgaccg gccctcaggc gcccttgctc
     7621 tatctagagc ctcgggatgg tcgtgcgcgt ggcgcgaacc ccccgcgaga gagcccgaaa
     7681 ccgggctctc cttcaatgca cgacgtcaag ccaacggaag atctgtagac acaacgcgga
     7741 agtgctggcc gccgccggga tcgtgaacac ccagccccaa acgatgttgc tggcgagacc
     7801 ccagcgaatg ccgcgcatgc ggttggtcga acccacgccg acgatcgcgc ccacgatggt
     7861 atgcgtcgtg gagaccggga tcttcatgtg agtggcgaag aacagcgtga gcgcgcccga
     7921 gatctcggcg cagaacgcgc tgtgggactt gagcttggtc aggcgtgagc caagggtctt
     7981 gatgatgcgc caaccgccga gcgcggtacc gagcgccatg gccgcgtagc aagacaaggc
     8041 gacccaagtc ggcgtctgct cgagattggg atcgatgtag ccgccggcga ccaacacggc
     8101 caagatgatg cccatcgtct tctgcgcgtc attgccgccg tgcccgatcg agtagcccgc
     8161 ggccgacagc agtcgtacga agcgcagcga gcggttcacc ttttgcgggc gcactctacg
     8221 caagacccag gacaacgcga ccagcagcag accgccgagc acgaagccca tcgtcggcga
     8281 gatcacgatg aactcggagg tgagcaccag cttggggatg ttcagcacct tcgcgccacc
     8341 gtatgcgaag cccgctccgg agaagccgcc catgatcgcg tgggacgagc tggatggcaa
     8401 cgcaagccac caagtgaaca tgttccaaac gatcgcgccg agcagccccg ccaagaccac
     8461 ccagacgaac ccgggatcgg ccgcgttcac cttgaccaaa ccggacacgg tcttggccac
     8521 accctggtgg aagatgaaga acgcgacgaa attgaagaac gcggcccacg ccaccgcgac
     8581 aggcggcgtc aacaggcgcg tagacacgat ggtggcgatc gaattggccg cgtcgtgaaa
     8641 accgttgatc aaatcgaaca gtaacgcgac cgcgatcgtg aagacgacga cggtatgaac
     8701 atccatga
//
LOCUS       DASTKU010000072         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_235528, whole genome shotgun sequence.
ACCESSION   DASTKU010000072 DASTKU010000000
VERSION     DASTKU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235528"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFQ61_02415"
     CDS             <1..290
                     /locus_tag="VFQ61_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9953325.1"
                     /translation="PQALALLREFQPEVAVLDIGLPVMDGYELALEIQRRMGAATPRL
                     IALTGYGQEHDVQRSRESGFQVHMVKPVDVSQLLDHITREGTSLSLRPPAH"
     gene            complement(343..1296)
                     /locus_tag="VFQ61_02420"
     CDS             complement(343..1296)
                     /locus_tag="VFQ61_02420"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET9953326.1"
                     /translation="MSAEVSASREFFWSEAPEPHAERRRRMLEAHPEIKQLYGPCPRT
                     KYVCTLLVVTQVATAYALRDAPWWLIVLVAYAFGGVINQSLFLAIHELSHNLAFKAPW
                     KNRVFALFVNLPAGIPVAATFRYYHLLHHVHQGEAPLDTDLPTRFEARWVKGPLRKAL
                     WVFGQGLAYGLRPLFVHPKRPDAWEIANFVVQMSFNVLVFWSWGPKAFWYLPIGSLLA
                     MGLHPIAGHYISEHYVFRPGQETYSYYGPLNRLAFNVGYHNEHHDFPYIPGSRLPKLR
                     ALAPEFYDNLMSHRSWTATLWQYITRPDLSGASRVRREVRG"
     gene            complement(1749..>2264)
                     /locus_tag="VFQ61_02425"
     CDS             complement(1749..>2264)
                     /locus_tag="VFQ61_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953327.1"
                     /translation="ERVGVVVRSPLRAFVDKQVSLARREQVVVTQELVGPDPNPSEIK
                     RAIRRLKKTSDILWILNDDRLLTPKLISEGWLPGLDERPWVPTIVGAGSLVSPSRSFG
                     TFAVLPDHVALGGQAASLLLDIADEGWTLEPNPFVQLPLSTTTTMDLNQVKERFAMQK
                     DALHQVDHILE"
BASE COUNT          442 a          723 c          700 g          399 t
ORIGIN      
        1 ccccgcaggc cctcgcgttg ttgcgggagt ttcagcccga agtcgcggtt ctagacatcg
       61 gactgcccgt gatggacggc tacgagctcg cgctcgaaat ccaacgccgc atgggcgccg
      121 ccaccccgcg gctgatcgcg ctcacggggt acgggcaaga acacgacgta caacgcagcc
      181 gagagagcgg ctttcaagtc cacatggtca aaccggtaga cgtctcccag ctcctcgacc
      241 acatcactcg tgagggaacg tcgctctcac tgcgcccgcc cgcgcactag cgctagtggg
      301 gtgttccata agtcgtgaaa tcatccacga gcaggctgcg actcagccgc gcacttctcg
      361 ccgcactcgg ctcgccccgc tcaagtcggg tcgcgtgatg tactgccaca gcgtcgcggt
      421 ccatgagcgg tggctcatga gattgtcata aaactccgga gccaaggcgc gcagcttcgg
      481 cagccgtgac ccagggatat acgggaaatc gtggtgctcg ttgtgatatc ccacgttgaa
      541 cgccaaacga ttcagcggcc cgtaatacga atacgtctcc tgccccggcc gaaacacgta
      601 gtgctccgag atatagtgtc cggcgatggg atgaagcccc atcgccagca atgaaccaat
      661 cggcaaatac caaaaagcct tgggacccca gctccaaaac acgagcacat tgaagctcat
      721 ttgcaccacg aaattggcaa tctcccaggc gtcggggcgc ttgggatgca cgaacagtgg
      781 gcgcaaaccg taggccaagc cctgaccgaa aacccagagc gccttgcgca gcggcccctt
      841 cacccaacgc gcctcgaagc gtgtcggcag atccgtatcg agcggcgctt cgccctgatg
      901 cacgtggtgc agcaagtgat agtaccggaa cgtcgccgcc accggaatac cggcgggcaa
      961 gttcacgaac agcgcgaaga cgcgattctt ccagggcgct ttgaaagcca agttgtggct
     1021 cagctcgtga atcgccaaga acaaagactg gttgatcacg ccgccgaacg cgtacgcgac
     1081 caagacaatc agccaccagg gcgcgtctcg caatgcgtac gccgtagcca cctgcgtcac
     1141 gaccaacaac gtgcacacgt acttggtgcg cgggcacggc ccgtacagct gcttgatctc
     1201 cgggtgcgcc tccagcattc ggcgtcgccg ctccgcatgc ggctcgggtg cttccgacca
     1261 aaagaactcg cggctcgctg acacctcggc ggacatggcg gggattgtaa cctcaacgcc
     1321 tgttcgtgcc cctccccgtg gaacgttcgg cgtgcctacc ccggaaaaac tcgggcgcat
     1381 ggccggagca tcagctccaa ctcaatcgag cctaggcgct tttgtggtag gcggcctcga
     1441 agcaacgggc gcgactgctc ggcgcgtcat ccacgtcagc gcagcccaat cgcccccgca
     1501 tcgcgtcacg ctaccgcgct cgccgcgctc tgtttattcg cgctctgttc attcgcgctg
     1561 tgtttgctac cgcggggttc gatgctgcca ttttgcgaga cgtcggggta atggggtgcc
     1621 gcatgcgcgg tgggcgcggg cactcacgag tgcatagcga ctgcgcggtt gggcaactcg
     1681 ccgagctgcg cgccaggtta ctccggggcg gccattccaa agccgatctt ggtcgcccga
     1741 agcccgcgtt attcgaggat gtggtcgacc tggtggagcg cgtctttttg catcgcaaag
     1801 cgctctttga cctggttcag gtccatcgta gtggtcgtcg agagtggcag ctggacgaag
     1861 gggttgggct ccaaggtcca gccttcgtcg gcgatgtcga gcaggaggct cgcggcttgg
     1921 ccgccgagcg cgacgtgatc cggcagcacc gcgaaggtgc caaacgatcg agagggggag
     1981 acgagtgagc ccgcgccgac gatggtcggc acccaggggc gctcatcgag gccaggcaac
     2041 cacccctcgg agatgagctt gggcgtcagc agtcggtcgt cgttcaagat ccacaagata
     2101 tcggaggtct tcttcaaacg tcgaatggcg cgcttgattt ccgaggggtt gggatcgggg
     2161 ccgacgagct cttgggtgac cacgacttgc tcgcgtcgcg ccaggctgac ttgtttatcg
     2221 acgaacgcgc gcagcggaga acgtacgacg acgccgacgc gctc
//
LOCUS       DASTKU010000073        15122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_682359, whole genome shotgun sequence.
ACCESSION   DASTKU010000073 DASTKU010000000
VERSION     DASTKU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 15122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15122
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682359"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFQ61_02430"
     CDS             <1..482
                     /locus_tag="VFQ61_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953328.1"
                     /translation="AKGKGEDAGGQGKGKGQGQPGGTRELEFVEVPSISQHMGAGSPS
                     GSSGEKAGKDASPGQENGSGREPGKGHDDNLQGDPTAPLAGQTQDVTAAGVDTGQGTA
                     SAEVIQGAAERGFVGKAYRDVYVQYETVAEQSLEHDNIPPGYRFYVRRYFQLIRPRE"
     gene            512..1513
                     /locus_tag="VFQ61_02435"
     CDS             512..1513
                     /locus_tag="VFQ61_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9953329.1"
                     /translation="MNETENVREQVAVFEQRLGRLREEIGRVIVGHRGVVDGVLTCLL
                     AGSHALLEGVPGLGKTMLVRTLASVLGLDFSRVQFTPDLMPADIIGTVVLDETADGKH
                     VFEFRRGPVFAHVVLADEINRATPKTQSALLEAMQEHRVTVGRTTYTIEEPYFVLATQ
                     NPLEMEGTYPLPEAQLDRFFFKLEVPFPSREDLHTILERTTTDHEATVRSVVTRGEVL
                     EMQALVRRVPAAPHVRDYAIRTLQATHPNGEGATKSVQRFVRTGASPRGVQAMLLAAK
                     VRALFHGRFAASIDDVREVAKPALRHRILLSFEGEAEGVRTDTLIEEILGSLPAAKD"
     gene            1538..2467
                     /locus_tag="VFQ61_02440"
     CDS             1538..2467
                     /locus_tag="VFQ61_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9953330.1"
                     /translation="MSFLGRIRSAPKAPEVKRAELFDDEFQRKLEMLSLLSRRIFSGQ
                     SRAERRSKKQGSGVEFADYRDYVPGDDFRYVDWQVYQRFARLLVRLYEEEEDLGIYFL
                     LDTSSSMGFGGGHKFDQARRLCAALAYVGLKNLDRITIVTLSDRLEERMPTTRGKGRI
                     FKVFQFLSGLETGGQTNLGDALRTFTLQHKRRGLAVLISDLYDLEGFEHGINVLRYNK
                     FEPYVLNVFDRKDALAGLKGDLSLYDCETGEARDVTVTDALLSRMERVLQEYRKEIET
                     FCTARKVPYFAADVDAKIEDLVLDMFRRGGFLH"
     gene            2467..4353
                     /locus_tag="VFQ61_02445"
     CDS             2467..4353
                     /locus_tag="VFQ61_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9953331.1"
                     /translation="MFGLHFAGASWATLLPWFAAAAVATTLLYFLKLRRRAVPVPFAK
                     LWERVLGDRESNQLFSQLKRWLSLLLQLIMLGLLFLALGDPRPVAQTARGRHIVVLMD
                     ASLSMQAVDEKPSRLERAKAEAKRFVRGLGNDDRAMIVQMDMHPLPLGPLTQEPSELS
                     AAIDQVRAQDTPADLGRALAFAEDSLRGLPNPEVLLVSDGALDVPESVPAKLKFTRIA
                     VGKSASNVAVIEFSARRYPLDKSRVEVLLEIENTNDRPAPIELTLYGDGAVIDVTHLN
                     LAANEKLSRVYSGIAGVNRKLEAQVRLANGKSDDLPRDDRAYALMPERRRARVLLVSS
                     GNTYLEAALLLDEYLDTTYVDAKAYPPKEAFDVTIFDGVAPTRAPHTGAALYLNPPKD
                     GSPVKLEAPLTDFGFDRWERKHPALKFLALGDVQVARGQRLRPEAGDQILGASDLGPI
                     LVAGSRSGAPFMVLGFDPRDSDFVLRPAFPLFVLGVIDSFLADDISYLSSYQTGENWR
                     VPTQSSATTAELFTPKNERLVVPVQAGRAAYFGNWAGFYRLSAQSPDQSEKSAHEFAA
                     NLGNSLESHIRPQPELKLGARTLQSASFGAPSIRRRSWAYFLIAVVALSIFEWFTYHR
                     RVTV"
     gene            4320..7163
                     /locus_tag="VFQ61_02450"
     CDS             4320..7163
                     /locus_tag="VFQ61_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9953332.1"
                     /translation="MVHVPPASHRMKLRRVLPLLLLWAALALVYYRYVWIADESAFQF
                     QRGGETFALLSPRALGVALVFPALLVGLWRSLADLPLAQRLLSLTLRVAFLSALAIAL
                     ANPVRTERTQRVCTVALLDVSDSVSDQALEEARKALAQMVREKPARDELRLITFAARP
                     RRVELDPADPTVIPGVAALRHRDAPSGTAAANTAPATPGTNPAAASTNIQAALELAYG
                     LYLPGYSRRAILLSDGVETEGDLLAEANRAKSFGVRLSAIPFRQPPPPEVSVTGLTLP
                     DKVDVGQTFSVRAQVYASRPSGAELRLFQGEALNGLDGVRQVQLQQGQNEIEFKSVVR
                     VGGDVTYALDLKPQGPDTFRENNNYKTTIEVPGRPLVLYVDGHPEHANYLANALGAQQ
                     FDVDLRAPSAFPGTLGEMERFDFVILSDAPREAFRPGTEELIERYVRDLGGGFLFAGG
                     PAGYGLGGWSHSTIERLLPVRMDAERRKEMPGVAMALVIDRSGSMSGLPMEMAKAACI
                     ASVSTLKGDDLLEVVAFDAEPVRYVKLQPVRYRSRIQADVQRIQPGGGTELFPALDMA
                     YQDLSVVQARKKHVILLTDGQAPTQGLRELAQAMLAEAITVTTVGLGGDVNQELLRTL
                     ADAGGGRFHAVPDPNSLPRIFTRETELVSQQAAVEEWFAVEQVGNADFLKGISIASAP
                     LLHGYVATELKPPPAQILLASDRGEPILARWRSGLGYALAWTSDVKNGWAVEWLRWSN
                     FGRFFGQLVREHMRVKRRKEVDMHVELEGSRVRAVVDAFTPDERFDNQWSSKLTLRTV
                     GSKSDPREAQFRKIAPGRYAADLELPGFGTYTLRADHTRRDEDGALKPLGVSFAHVSN
                     PYPREYARLEPDVERLSRAALATGGELDPTPARLFDPRGEHLLHNREHYPAFVAGAVL
                     LFLLDLLIRRVRLFDRKHLRAQPGRSLHPRAGA"
     gene            7596..8105
                     /locus_tag="VFQ61_02455"
     CDS             7596..8105
                     /locus_tag="VFQ61_02455"
                     /inference="COORDINATES: protein motif:HMM:NF020022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9953333.1"
                     /translation="MELVILVGLQAAGKSTFRRSRFDGTHVVVSKDELRNNRRPARRQ
                     EQLIRTALAAGRSVIVDNTNSRQEDRAQLVALAREYSAKAVAYFLPLSVPESIRRNAR
                     REGRERVPDIGVYATARVFTAPTWEEGFDELYEVLVEDDHSIVIRSMSREPGASTGTA
                     VPPLDAKIS"
     gene            8130..8897
                     /locus_tag="VFQ61_02460"
     CDS             8130..8897
                     /locus_tag="VFQ61_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008193 - tRNA guanylyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HET9953334.1"
                     /translation="MAENDDLEARMRALEWFHALRVVPATWPVIRVDGRGFTRLVEQY
                     FDRPFDVRFHQIMVQTARTLLQELHGVYAFTESDEISVVLHPDTDLFHRELEKLVSIS
                     AGVASATFSLELGKPAHFDGRVWMGPRLEHVVDYLRWRQSDAARCALNTWCYWSLRKE
                     GQTYEQVTATLTGKNFSDKNELLFARGINFNDVPLWQKHGVGVYPKRIEKQGLNPMTG
                     EVTLAQRRQVVVDTELPRGDAYAEFITQLLQSATRSA"
     gene            complement(8946..9752)
                     /locus_tag="VFQ61_02465"
     CDS             complement(8946..9752)
                     /locus_tag="VFQ61_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953335.1"
                     /translation="MDARVHIVKAGETPQILEKLYSATWQEIRECPGNVFLKQRKGYP
                     LHPGDRVHIPNRPAAKPTEAPGFTQPFEEFLALVNANMKLLLALTNQHPARSSVRVYS
                     TALPNKHPGVSATKAADVVGYKKSLPKVRSSVVLTEPIESAFTVLSDTLPVGAVMTSG
                     YRSDADQARVINDYFAKFNGPGAISEVEARRQWLLALKDENGKSKGLKIAAVGCSPHR
                     TGLAFDLSGASLDAIERAVTQCAQTYPDKFPLLNTILERNQNCLHVNLKH"
     gene            10034..10954
                     /gene="ligD"
                     /locus_tag="VFQ61_02470"
     CDS             10034..10954
                     /gene="ligD"
                     /locus_tag="VFQ61_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET9953336.1"
                     /translation="MKYRPQLAELAETPPRGPGWLTEIKHDGYRIGILLQKGEVRLES
                     RRNLDWTRKLQPIADAARSLAVSSLVMDGEAVILRENGLSDFQALQNTWRAAGPLTGL
                     CFFGFDLLQLDGEDLTRTPLRERKALLERVLRAPANPVLRYSEHLENEPSAVLEAARK
                     LGAEGVVCKRADGVYHPGRHHSWLKIKCTKSGEFLICGFTEPEGSRQGVGGLLLGRYR
                     NGELVFRGSVGTGHGWTAEFLSELRRGLETLRQSDCPFSNKPPVPRAKWVRPALVCEV
                     SYVEITTDGSLRHPSFRGFRPDKSAREVKE"
     gene            11095..12006
                     /locus_tag="VFQ61_02475"
     CDS             11095..12006
                     /locus_tag="VFQ61_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9953337.1"
                     /translation="MRSPSERDDNLNTAADAYRARFRRVLDHIDAQLDAPLSVEDLGA
                     VAAFSKYHFHRQFSAWIGMSVFRYVQLLRLKRASYQLAFREQSRILDIALECGYESNE
                     AFSRAFKRAFALTPSEFRERPDWEAWRATYEALKEIRSLHMRPELRAGDVRLVDFQET
                     RVAALEHRGDPATIEDSIRRFIEWRRQNRLHPKLSATFNVFWDSPSDTAPERFRMDLC
                     AAITGSIPENSHGVVPKTIPGGRCAVLRHVGSEDTLPESISFLYSQWLPSSGEELRDF
                     PLYLQRVTFFPDVPEHEAITDIFLPVR"
     gene            12367..14142
                     /locus_tag="VFQ61_02480"
     CDS             12367..14142
                     /locus_tag="VFQ61_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953338.1"
                     /translation="MSFSSLRLAMSVSLLGAFACSSGGIGEDPPQPPAGGGASGGSTQ
                     SGGGSQSGGASASGGNAQGGSSGNTQAGANTGGSARGGAMNGGSNAGGQTGARGGGMN
                     GGSAQGGSTSAGGAAQGGSTAAGGNAAGGANGGSTSGGAANGGSATGGGANGGSANGG
                     SAGSPGTCGPIADQLSSVEVTTPEGVRAGVRNWRVWASTSLNIAPVFTTPLANCGTLV
                     CYTTGSGSTRRARVTRLDAQDKLVETYDLGAYECRGLAAEPDGHFGALLWEPGTKTDC
                     ADAEASGSIYVSRFDATGKSLWSKELTNLSTSGLGPNCPTEFGIGESRLEFGGGKYGA
                     YYHVHSKSGHEGDTLKYVDLSGTESTTWSWGCSHSMSNVLRFNPTDSKFLPACVTDCY
                     PGTGDGDFAAVSIGGIYLNNKTKVLDVDGGCNGSVAGELGGGAIAPSGWKLVFNAHQN
                     PATLGQKSYNTSTMNQDIGFASVSSSFAPGAVKWLTNTPSIQEADSSIERLKLACDPT
                     EQYLVGWAEPGSTYKYKLGRINANGEFLEAATDVTAKAKWGRRDDPFRAHANGDIVWA
                     WFDAPNSTTLHFARVRSGGTAQCGN"
     gene            complement(14204..>15122)
                     /locus_tag="VFQ61_02485"
     CDS             complement(14204..>15122)
                     /locus_tag="VFQ61_02485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9953339.1"
                     /translation="AQGPLSSGLLERVERAAREFDVLVFFTYLYHPTVAGLPLAGRKS
                     LLIPTAHDEPAIHFRIFRRVFESPTAIAFNSVEERDFVLSHFDVRAPYSDIVGCGVEL
                     GGSPERLQEERSAPDRPFVLYLGRIAKNKGVPHLVACFRRFKAENEQRYFEGAHGKYL
                     GRELELVIAGSGETGLIQANADVRLAGFVSDAEKSNLLRDTEALMMPSFYESLSLVTL
                     EAFAWGRPVLAQSRCAVTRGHIERSGAGDTYADEAEFSRKLSEMLGNAALRAERGERG
                     RAYVRANYAWEKVESRLLRLLQLTAAAAR"
BASE COUNT         2819 a         4798 c         4818 g         2687 t
ORIGIN      
        1 gagcaaaggg caagggcgag gatgcggggg gccagggcaa aggaaaaggt cagggccaac
       61 ccggtggaac tcgcgagctc gagttcgtgg aggttccgag catcagccaa cacatgggtg
      121 ctggcagccc ctccggatcc agcggcgaaa aagccggaaa ggacgcgagc cccggccaag
      181 agaatggctc tgggcgcgag ccgggcaaag gccacgacga caatttgcaa ggagatccca
      241 ccgcgccgtt ggccggccaa acccaagacg tcacagccgc gggggtcgac accggccaag
      301 gcaccgcctc ggccgaagtg atccaaggag cagccgaacg cggtttcgtc ggcaaagcgt
      361 accgcgacgt gtacgttcaa tacgagacgg tcgccgagca gtcgctcgag cacgacaaca
      421 tcccccctgg ctaccgcttc tacgtgcggc gctacttcca actcatccgc ccccgagagt
      481 gatcaggcgg tggggcttca aggagtgacg agtgaacgaa actgaaaacg tccgcgaaca
      541 agtcgctgtc ttcgaacaac gcctgggccg gctgcgggag gagatcgggc gggtcatcgt
      601 ggggcatcgg ggggtggtcg acggggtgct gacgtgcctg ctcgcggggt cgcacgccct
      661 gctcgaaggg gtacctggcc tcggaaagac gatgctggtc cgcacactgg cctcggtgct
      721 ggggctggac ttcagccgcg tacagttcac gcccgacctg atgccggcgg acatcatcgg
      781 cacagtggtg ctcgacgaga ccgccgacgg taaacacgtc ttcgagtttc gacgtggacc
      841 ggtgttcgcg cacgtggttc tggcagacga gatcaatcgc gccacaccaa agacccagag
      901 cgcgttgctc gaagccatgc aggagcatcg ggtgaccgtg ggtcgcacca cgtacaccat
      961 cgaagagccc tacttcgtgc tcgcgaccca gaaccctcta gaaatggagg gaacctaccc
     1021 gttgcccgag gcgcagctcg accgcttctt cttcaagctc gaggttcctt tcccgtcccg
     1081 ggaggacctg cacaccatct tggagcgcac cacgaccgac cacgaggcca ccgtccggtc
     1141 ggtggtgaca cggggcgagg tgttggagat gcaggccctg gttcggcgtg tgcccgcggc
     1201 cccgcatgtt cgcgactatg cgattcgaac gctccaggcc acgcatccca acggcgaagg
     1261 tgcgaccaaa tcggtgcagc gcttcgtccg aaccggagcg tccccgcgcg gtgtgcaagc
     1321 catgttgctc gcggccaagg tgcgggcact gttccatggt cgcttcgccg catcgatcga
     1381 cgacgttaga gaagtggcca aaccagcgct gcgccaccgc atcctcctca gcttcgaggg
     1441 cgaagccgag ggcgtccgca ccgacacgtt gatcgaagaa attcttggca gcctgcccgc
     1501 cgccaaagac tgaccccgcg gagggctcgc gaccatcatg agttttcttg gacgtattcg
     1561 atccgcgccg aaggctccgg aggtcaaacg cgcggagctg ttcgacgacg agttccagcg
     1621 caagctcgag atgctttcgt tactcagccg acgcatcttc tcgggtcaga gccgagcgga
     1681 gcgacgcagt aagaaacaag gtagcggagt cgagttcgcc gactatcgcg actacgtgcc
     1741 cggggatgac ttccgctacg tggactggca agtgtaccaa cgcttcgcgc ggctgctcgt
     1801 gcggctctat gaagaggaag aggacctcgg catctatttc ctgctcgata cgtcgagttc
     1861 gatggggttc ggcggtggcc acaagttcga ccaagcccga aggctgtgcg ccgcgctggc
     1921 gtacgtaggt ttgaagaacc tcgatcgcat caccatcgtc acgctctccg atcgcttgga
     1981 ggagcgcatg ccgacgacgc gcggcaaggg gcgaattttc aaggtcttcc aattcctctc
     2041 cgggctcgag acgggaggcc aaaccaatct gggggacgcg ctgcgcacct tcaccctgca
     2101 gcacaagcgt cgcgggctcg ccgtcttgat cagcgacctc tacgacttgg agggtttcga
     2161 gcacggcatc aacgtgctgc gatacaacaa gttcgaaccc tatgtgctga acgtcttcga
     2221 tcggaaggat gcgctcgccg gactcaaggg tgacttgagc ttgtacgact gcgaaaccgg
     2281 tgaagcgcgc gacgttacgg tgaccgacgc tttgttgtcc cggatggagc gggttctgca
     2341 ggaatacaga aaggagatcg agaccttctg cacggcgcgt aaggtgccct acttcgccgc
     2401 ggacgtggac gccaagatcg aagatctggt gctcgacatg tttcgccgcg ggggatttct
     2461 gcactgatgt tcggtttgca tttcgcaggc gcgagctggg cgacgctatt gccttggttc
     2521 gcggcggccg ccgtggcgac cacgctgctc tacttcttga agttgcgtcg ccgcgccgtt
     2581 ccggttccgt tcgccaagct gtgggaacgg gtgctcgggg atcgcgagag caatcagctg
     2641 ttctcgcagc tcaagcgctg gctgtcgctg ctgttgcagt tgatcatgct ggggctgctg
     2701 tttctcgcgc tcggtgatcc gcgcccggta gcgcagacgg ctcgtggtcg gcacatcgtc
     2761 gtgctgatgg acgcgagctt gtcgatgcag gccgtcgacg agaaaccatc gcgcctcgag
     2821 cgggccaagg ccgaggccaa acgcttcgtg cgcggactcg gcaacgacga ccgcgccatg
     2881 atcgtgcaga tggacatgca tccgctgccg ctcggcccgc taacccaaga gccaagcgag
     2941 ctcagcgccg cgatcgatca ggtgcgggcg caggatacgc cggcggacct aggtcgggca
     3001 ctcgcgtttg cggaagattc cttgcgcggg ctgccgaatc ctgaggtgtt actggtatcc
     3061 gacggcgcgc tcgacgtgcc cgagagcgtg cccgcgaagt tgaagttcac gcgcatcgcg
     3121 gtcggcaaga gcgcctcgaa cgtcgcggtg atcgagtttt ccgcccggcg ctacccgctg
     3181 gacaagtcgc gtgtcgaggt gttgctcgag atcgagaaca ccaacgaccg cccggcaccg
     3241 atcgaactca cgctgtacgg ggacggcgcg gtgatcgacg tcactcacct caacttggcc
     3301 gcaaacgaga agttgtcgcg cgtctactcc ggcatcgccg gtgtaaaccg gaagctcgaa
     3361 gcgcaggttc gcctggccaa cggcaagagc gacgacctgc cccgagatga ccgtgcgtac
     3421 gccttgatgc cagagcgacg ccgcgcgcgc gtgttgctcg tcagctcggg caacacttac
     3481 ttggaagcgg cgctcctgct cgatgaatac ctggacacga cgtacgtcga cgccaaggcc
     3541 tatcctccca aggaagcttt cgatgtcacg atcttcgacg gcgtggcgcc cacgcgcgcg
     3601 ccacacaccg gggccgcgct ctatctgaat ccacccaaag acggcagccc cgtcaaactc
     3661 gaggctccgc tcacggattt cggtttcgat cgttgggaac gcaagcaccc tgcgctcaag
     3721 ttcctggccc tcggagacgt gcaggtggcg cgtggccaac gtttgcgtcc cgaggctgga
     3781 gatcaaatcc tgggcgccag cgacttgggc ccgatcttgg tcgccggtag ccgcagcggc
     3841 gcgccgttca tggtgctcgg cttcgaccct cgcgatagcg atttcgtgct gaggcccgcg
     3901 tttccgctgt tcgtactcgg cgtaatcgat agcttcttgg ccgacgacat cagctatctc
     3961 tcgtcctatc agacgggtga gaattggcgc gttccaaccc agagctcggc cacgacggcc
     4021 gagctcttca ccccaaagaa cgagcgcttg gtggtccccg tgcaggcggg acgagccgca
     4081 tacttcggca attgggccgg cttttaccgg ctctcggcgc aatcgccaga tcaatccgaa
     4141 aaatccgctc acgagttcgc cgccaacctg ggaaacagcc tggaaagcca tatccggccg
     4201 cagcccgagc tcaagctcgg cgcgcgaacg ctgcagagcg cgagctttgg ggcgcctagc
     4261 atccggcgcc gctcgtgggc ttactttcta atcgcggtgg tagcgctttc gatcttcgag
     4321 tggttcacgt accaccggcg agtcaccgta tgaagctgcg ccgggtctta cctctgctac
     4381 tgctttgggc agcgctcgcg ctggtctact accgctacgt ctggatcgcc gacgagagtg
     4441 cgttccagtt tcagcgcggc ggtgagacat ttgcgctgct ctcgccgcgt gcactcggcg
     4501 tggctttggt gtttcctgcg ttgctcgtgg gtttatggcg ctcgctcgcc gacctgccgc
     4561 tcgcgcagcg tctgctctcg ctcaccctgc gcgtcgcgtt cttgagtgcg ttggcgatcg
     4621 cgctcgccaa cccggtccgc acggagcgca cccagcgcgt ctgcacggtc gccctgctcg
     4681 atgtgagcga ctccgtttcg gatcaagcgc tcgaagaggc ccgcaaggcc ttggcgcaga
     4741 tggtccgcga gaaacccgcg cgcgacgagc tgcgcttgat caccttcgcc gcacgccccc
     4801 gtcgcgtgga gctcgacccc gcagatccga cggtaattcc aggcgtggcc gcgctccgcc
     4861 atcgcgacgc gccgtccggc accgccgcgg ccaacacggc gcccgccact ccgggcacga
     4921 accccgcagc cgcaagcacc aacatccaag cggcgctcga gcttgcttac ggcctttacc
     4981 tacccggcta ctcacggcgc gcaattctgc tcagcgacgg cgtggaaacc gagggtgatc
     5041 tgctcgccga agcaaaccgt gccaagagct tcggcgtccg gctgtcggcc attccgtttc
     5101 gccagccgcc accgcccgag gtgagcgtca ccgggctcac tttgccggac aaggtggatg
     5161 tcggccagac cttctccgtc cgtgcacaag tttacgccag ccgaccgagc ggcgcggagc
     5221 tgcggttatt tcaaggcgag gctttgaatg gcttggacgg tgtgcgccaa gttcagctgc
     5281 agcaaggcca gaacgagatc gagttcaaga gcgtcgtgcg cgtcggaggt gacgtcacct
     5341 acgcgctcga tctcaaaccg caagggccag acaccttccg cgagaacaac aactacaaga
     5401 cgacgatcga ggtgccaggc cgccccctcg tgctgtacgt ggatgggcac cccgagcacg
     5461 ccaattacct ggctaacgcg ctcggggccc aacagttcga cgtggatttg cgggcaccga
     5521 gcgcgttccc cggcacgctc ggtgagatgg agcggttcga cttcgtcatc ctctccgacg
     5581 ccccacgcga agccttccgc cctggaacgg aggagctgat cgaacgctac gtgcgtgatc
     5641 tcgggggcgg cttcttgttc gcaggtggcc ctgccggcta cggcctgggc ggttggtctc
     5701 acagcacgat cgaacggctc ttgccagtgc gcatggatgc cgagcgacgc aaagaaatgc
     5761 ccggcgtcgc catggcgctc gtcatcgatc gctcgggttc gatgtcgggc ttgccaatgg
     5821 agatggccaa ggccgcatgc attgccagcg tctccacctt gaagggtgac gacttgttgg
     5881 aggtggtcgc attcgacgcg gagccagtgc gttacgtcaa gctccagccc gtgcgctatc
     5941 gcagccgcat ccaagccgac gtgcaacgca tccagcccgg cggcggcacc gagctcttcc
     6001 ccgccctgga catggcgtat caagatctgt ccgtcgttca ggcccgcaaa aagcacgtaa
     6061 ttttgctcac cgatgggcaa gctccgaccc agggcctgcg cgaactcgcc caagcgatgc
     6121 tcgccgaagc gatcacggtt acgacggtgg gcttgggcgg ggacgtgaac caagagcttc
     6181 tacgcacgct ggcggatgcg ggcgggggcc gcttccacgc cgtgcccgat cccaacagct
     6241 tgccacgcat cttcacgcgc gagaccgagc tcgtatcgca acaagctgcg gtcgaagaat
     6301 ggttcgcggt ggagcaggtt ggcaacgccg acttcttgaa aggcatttcg atcgcgagcg
     6361 cacctcttct gcacggctac gtggccaccg agctcaagcc accacccgcc caaattttgc
     6421 tcgccagtga ccgcggtgag cccatcctgg cgcgctggcg gagtggcctc ggttatgcgt
     6481 tggcctggac ctccgacgtc aagaacggct gggccgtcga gtggctgcgc tggtcgaatt
     6541 ttggccgatt cttcgggcag ttggtgcgtg aacacatgcg cgtcaagcgt cgcaaagagg
     6601 tcgatatgca cgtggagctc gaaggctcgc gcgtgcgcgc ggtcgtggac gccttcacgc
     6661 cggacgagcg cttcgacaat cagtggtcat cgaagctgac gctgcgcacc gtgggcagca
     6721 aatccgaccc gcgcgaggcg cagttccgga agatcgcgcc aggccgctat gccgcagatt
     6781 tggagctgcc cgggtttgga acatacaccc tgcgcgcaga tcacacgcgc cgcgacgagg
     6841 atggagcact caagcccttg ggcgtcagct ttgctcacgt ttcgaacccg tacccgcgcg
     6901 agtacgcgcg tctcgaaccc gacgtggagc ggctcagccg agccgcgttg gccacgggcg
     6961 gagaactgga tcccactccc gcgcggctgt tcgatccgcg cggcgaacat cttctgcata
     7021 atcgagagca ctaccccgcg ttcgtcgcgg gggccgtgct cctgttcttg ctcgatctct
     7081 tgattcgtcg cgtgcggctg ttcgaccgca agcatctccg cgcccaacca ggccgatcat
     7141 tgcacccgcg cgcgggcgcg tgaaagcgtc acgcttggag cgggcgcgcc cgcccatcaa
     7201 cgcggcttca gtgccgcgcg cctgccgatg gagggccggg caagataggg tgagagctac
     7261 gccgactttc cggggggcgc gcccgacaac cctcgagcgt ctgcgcgctg cgcttgtgct
     7321 tgggcctgca gacaccagag ccgcgaactg agtcaaagtt cgacactccg tagttcgaaa
     7381 aactcggtcg ctcgaaactg atgtggcgaa ggacgccagc cgtctcccca ctttggctcc
     7441 gggcgcgggt cgtttcgagc atggagcgga gccgatcccg ctcgatgcgc gcagcgtcgc
     7501 gtggcgcgcc ttgccccgcg cgcggcatga gcgtgaagta agtacccaca ctcgttttgt
     7561 ctcccaccct ggcggatgcc acgtatctta gagcaatgga attggtgatc ctcgtgggct
     7621 tacaggccgc gggcaagtcc acctttcggc gcagccgctt cgacggaacg cacgtcgtcg
     7681 tgagcaagga cgagctgcgc aacaataggc gccccgcgcg ccgccaagaa caactgattc
     7741 gaacggcgtt ggcggccggc agatccgtga tcgtggacaa caccaactcg cgccaagaag
     7801 atcgcgctca actcgtcgcg ctggctcgcg agtactcagc caaagccgtc gcctatttct
     7861 taccgctcag cgtaccagag tcgatccgcc gcaacgcgcg acgcgagggt cgcgagcgag
     7921 taccggatat cggcgtgtac gccaccgcgc gcgtgttcac ggcgccaacc tgggaagaag
     7981 gcttcgacga actctacgaa gtgctcgtcg aggacgacca cagcatcgtg atccgcagta
     8041 tgagccgaga gccaggcgcc tcgaccggca cggccgttcc accgttggat gccaaaatca
     8101 gctgaactcg gctaggcttc ggcagaccca tggccgagaa cgacgaccta gaagctcgca
     8161 tgcgagcgct cgagtggttc catgcgcttc gcgtagtccc cgcaacctgg cccgtgatcc
     8221 gcgtagacgg ccgtggcttc acgcgcctag tggagcagta cttcgatcgc ccgttcgacg
     8281 tgcgctttca ccaaatcatg gtgcagacgg cacgcaccct tttgcaggag ctgcatggcg
     8341 tctacgcctt caccgagagt gacgagatct cggtcgtgct ccaccccgac accgacctgt
     8401 ttcaccgcga actggagaaa ctagtgtcca tttcagcggg ggtggccagc gctaccttca
     8461 gcctcgaatt aggaaagccc gcgcacttcg atggtcgagt ttggatgggg ccgagattgg
     8521 aacacgtggt ggattatctt cgctggaggc agtccgatgc cgcgcgctgt gccctgaata
     8581 cgtggtgtta ctggagcttg cgcaaagaag ggcaaactta cgaacaagtc acggccaccc
     8641 tcaccggcaa gaacttttcg gacaagaacg agctcctgtt cgcaaggggg atcaacttca
     8701 atgacgtacc cctttggcaa aaacacggcg tgggcgtgta tccgaagcgg atcgagaagc
     8761 aggggctcaa ccccatgacg ggcgaagtca ccctggccca acgtcgccaa gtcgtggtcg
     8821 acacggagct tccgcgcgga gacgcttacg cggagttcat cacgcaactc ttgcagagcg
     8881 cgactcgctc cgcgtgactc gaggcctcac ggaccgcgtc acgcgcggcc cggcgtgcgt
     8941 gcgcctcagt gcttgaggtt gacgtgcaaa caattctgat tgcgctcgag gatcgtgttc
     9001 aacagcggaa acttgtctgg atacgtctgt gcacactgcg tgacggctcg ctcgatggca
     9061 tccaggctcg cgccagaaag atcgaaggcc aagccggtgc ggtgcgggct acagccgacg
     9121 gcggcaatct tcaggccttt gctcttgcca ttctcgtcct tcaacgcgag caaccattgg
     9181 cggcgcgcct cgacttcgct gattgcacct ggtccgttga acttggcgaa gtagtcgttg
     9241 atcacacgag cctggtcggc gtccgagcgg taccccgacg tcatcaccgc acccacgggt
     9301 agggtatccg aaagcaccgt gaacgcggac tcgatcggct ccgtcaacac cacgctgctc
     9361 cgcactttgg gcagcgattt tttgtatccg acgacgtcgg cagccttggt cgcactcact
     9421 ccggggtgct tgttcggtaa cgccgtagag taaactctga ccgagctccg cgcgggatgt
     9481 tggttggtga gcgccaacag cagcttcatg ttcgcgttga ctagcgcgag gaactcttcg
     9541 aaaggctgag tgaagccagg tgcctcggtc ggtttcgccg ccggccgatt cgggatgtgg
     9601 acgcgatctc ccgggtgaag agggtaaccc ttccgctgct tgaggaagac gttaccgggg
     9661 cactcgcgga tttcctgcca cgtcgcggag tagagcttct ccaggatttg aggagtctcg
     9721 cccgctttca cgatgtgaac ccgcgcatcc atctttccaa tgctcctttc gcgacttcct
     9781 gcccaccagc tcgctcgtca ggatagcgag cgacgaagtc gaccgaaagg acggatctca
     9841 tcaccacgcc gaacgcggag cggctccgag agtgcgcgaa agtggcagcc ctccgcgtcg
     9901 gcggatctcg ccggcccgtt cgcgcggaat gggccacgcc tcggaatgga acatcgcttc
     9961 cgctccgccg cgctgctcac acacgcgacg ataccgtgcc cgacgcggta aactgcgccg
    10021 aggtcatacg ctcgtgaagt accgcccgca gctcgcggag ctcgccgaga cgccaccccg
    10081 gggcccgggc tggctgacgg agatcaaaca cgatgggtac cgaatcggta tcctgctcca
    10141 gaagggcgaa gtacgcctgg agtcccgtcg caacttggac tggactcgga agctgcagcc
    10201 catcgccgat gccgcccgct cactcgcggt gagctcgttg gtcatggatg gcgaggccgt
    10261 aatcttgcgc gaaaatggcc taagtgactt ccaagcgttg cagaatacct ggcgcgccgc
    10321 ggggccactg accggcctgt gcttctttgg gttcgatttg ttgcaactgg atggggaaga
    10381 cctgacgcga accccactgc gcgagcgcaa ggccctgctg gagcgagtgt tacgcgcgcc
    10441 ggccaacccg gtgcttcgct actccgagca cttggaaaac gaaccttctg cggtcttgga
    10501 ggccgcacgg aagctgggcg ctgaaggcgt ggtctgcaag cgggcagatg gcgtttacca
    10561 ccctggccgc catcactctt ggctaaagat caaatgcacg aagagtggag agttcttgat
    10621 ctgtggattc acggaaccgg aaggctcacg ccaaggtgtg ggaggactac tgctcgggcg
    10681 gtatcgcaat ggagagctcg tgtttcgagg tagcgtcggc acgggccacg gctggacggc
    10741 agagttcttg tccgagctgc gacgcggtct cgaaaccctg cgtcagtccg attgtccctt
    10801 ctcgaacaag ccgccggtcc cccgggccaa gtgggtccgc ccagctctgg tttgcgaagt
    10861 cagctacgtc gagatcacga cggacggctc cttgcggcac ccttcgttcc gaggcttcag
    10921 acctgacaag tctgcccgcg aggtaaagga gtagccgcgc cgcgcgtggc gagcaccaaa
    10981 gccggcgagc tacaggttcg gtcggagagc ccgcgcacgc gagcactttc cgcaacttcg
    11041 gtcaggcgaa gacgcgtgta aacgaatacc aatcgaagcc gcaccaagtc actgatgcgc
    11101 tccccttctg aacgagacga caacttgaat accgctgctg acgcataccg cgcccgtttc
    11161 cgtcgagtgc tggaccatat cgacgcgcag ctcgacgcgc cgctttcagt cgaagatctg
    11221 ggcgcggtgg cggcgttctc gaaataccac tttcatcggc aattctcggc ttggattggg
    11281 atgagcgtgt tccgctacgt gcagttgcta cgtttgaagc gcgcctcgta tcaactggca
    11341 tttcgcgagc agagtcggat cctggatatc gctctcgagt gtggttacga gagcaacgag
    11401 gcgttctcgc gcgcgttcaa gcgggcgttc gcgcttacgc cttccgagtt ccgcgaacgg
    11461 cccgattggg aggcttggcg tgcaacgtac gaggcgctga aagagataag gagtttgcac
    11521 atgagaccgg aattgcgagc aggcgacgtg aggctcgtgg attttcagga gacgcgcgta
    11581 gcggcgctcg agcaccgggg tgacccggcg acgatcgaag actcgatccg tcgcttcatc
    11641 gagtggcgcc ggcaaaacag acttcacccg aagctcagcg ccaccttcaa cgtgttttgg
    11701 gacagcccat ccgatacggc gccggagcga ttccgaatgg atctctgtgc ggccatcacc
    11761 ggatccatcc ccgaaaactc ccacggggtg gtgcccaaga cgatccccgg cggccgttgc
    11821 gcggtgctgc gccacgtcgg ttcggaggac accttgcccg agtcgatctc gttcttgtac
    11881 tcgcagtggc tgccgagcag cggcgaggag ctgagagatt tccccttgta tctgcagcgg
    11941 gtgacgttct ttccggatgt acccgaacat gaggcgatca ccgatatctt cctccccgta
    12001 cgctgatcac tcgggcctgc atcggaaaac tccgctcgca gcgtgcgaga ttggcaatga
    12061 ccaaccggcg cggcgatgaa ccatgtgtcg aaccgcgcag tcggcccagg tgatgccctg
    12121 agtctgctct agttggtggc ccccgatgat cgccgtttat ccctgaaaca acgcactctg
    12181 cgcgctcgac ggagcaacga gactggctca gaatgtcgac ggctgagaat tctgagctgg
    12241 tcgagttgct tccagcaact cttccgtacg cgcttcgagc gtcgtacgca ctccccgggc
    12301 ggcgaacccg cgctcggggt cgtgagagtg gtcgcgccgc tggcgcggtc ggaaaggtcg
    12361 cctctgatga gtttttcgag tttgcgcctc gctatgagcg tctctctact tggtgccttc
    12421 gcgtgcagct cgggtgggat tggcgaagat cccccgcagc cgcccgctgg aggaggtgcg
    12481 agcggcggca gcacccagtc tggcggaggg agccagtcgg gaggagcaag cgcgtccgga
    12541 ggcaacgccc aaggcggctc tagcgggaac acccaagccg gcgcgaacac gggcggctcg
    12601 gcccggggcg gagcaatgaa cggcggcagc aacgccggcg ggcaaacggg agcccgcggc
    12661 ggtggaatga acggcggctc ggcgcagggc ggatcgacct cggccggcgg cgcggcgcag
    12721 ggtggatcga cggcagccgg cggtaatgcg gccggtggcg caaacggcgg ctcgacgagc
    12781 ggcggcgcgg ctaacggcgg tagtgctact ggcggcggag ccaacggcgg cagtgccaac
    12841 ggcgggagtg cgggttcacc tggaacgtgc gggccaatag ccgaccagct gagcagcgtt
    12901 gaagtcacca ctccggaggg tgtacgggct ggcgtacgta actggcgcgt ttgggcctcc
    12961 acttcgctca acatcgcgcc cgtgttcacg actcccctcg cgaactgcgg cacgctcgtg
    13021 tgttacacca cgggcagcgg ttctacccgt cgagcgcgag tgacgcggct cgatgcgcaa
    13081 gacaagctcg tcgaaaccta cgatctcggt gcttacgagt gccggggcct cgcggcagag
    13141 cccgacggac atttcggggc gctgctgtgg gagccgggca ccaagacgga ctgcgcggac
    13201 gccgaggcca gcggcagcat ctacgtctcc cgattcgatg cgaccgggaa gtccttgtgg
    13261 tccaaggagc tcaccaatct gagcaccagc ggtctgggcc cgaattgccc gaccgagttc
    13321 gggatcggag agagccggct cgaattcggc gggggcaagt acggcgccta ctatcatgtc
    13381 cactccaaat ccgggcacga aggcgacacg ctgaagtacg tcgatttgag cggtaccgag
    13441 agcaccacct ggagttgggg atgttctcac agcatgagca atgtgctccg cttcaacccg
    13501 acggactcca agttcttacc cgcgtgcgta accgactgtt accccggtac cggagatggc
    13561 gatttcgcgg cggtgagcat cgggggcatc tacttgaaca acaagactaa ggtgctggac
    13621 gtagacggag gttgcaacgg aagcgtcgcg ggcgagttgg gtggaggagc gatcgcgccg
    13681 agcggctgga agctcgtctt caacgcgcac cagaaccccg ccacgctcgg tcagaagtct
    13741 tacaacactt cgaccatgaa ccaggacatt ggcttcgcca gcgtgtcgtc gagctttgca
    13801 ccgggcgcgg tgaagtggct cacgaataca ccctcgatcc aggaggccga ttcgagcatc
    13861 gagcggctca agctcgcttg cgacccgacc gagcagtacc tggttggctg ggcggagcct
    13921 ggctccacct acaaatacaa gctcggacga atcaacgcga acggcgagtt cttggaagcg
    13981 gccaccgacg tgaccgccaa agccaagtgg ggccggcgtg atgatccgtt ccgtgcccac
    14041 gcaaacgggg acatcgtgtg ggcctggttc gacgcaccga acagcaccac ccttcacttc
    14101 gcccgcgtgc gctccggagg caccgctcaa tgcggcaact aaccgcgcag tgcggaaact
    14161 aaccgctcaa tgcggcaacc aataggcaca gggcccgcca ggatcagcgg gccgctgccg
    14221 ccgtgagctg cagcaatcgc agcaagcgac tttccacttt ttcccaagcg tagttggctc
    14281 gaacgtacgc tcgcccgcgt tcgccgcgct ccgcacgcag cgcggcgttc ccgagcatct
    14341 cgctcagctt gcggctgaat tctgcctcgt cagcataggt gtcaccggcg ccggagcgtt
    14401 cgatgtggcc gcgcgtgacc gcgcaccggc tctgcgcgag cactgggcgc ccccacgcga
    14461 aggcctcgag ggtgaccaat gacaagcttt cgtaaaagct cggcatcatc agcgcctcgg
    14521 tatcccgaag caggttggac ttctcggcat cggagacgaa ccccgcgagc cgcacatccg
    14581 cgttggcttg gattaggccg gtctcaccgc tacccgcgat cacgagctcg agctcgcgcc
    14641 ccaggtactt gccgtgtgca ccttcgaaat agcgctgctc gttctcggct ttgaagcgac
    14701 ggaaacatgc gacgaggtgg ggcacgccct tgttcttcgc gatgcgcccg agatagagca
    14761 cgaacgggcg atcgggcgcc gagcgctctt cctgcaggcg ttctgggctg ccgccgagtt
    14821 cgactccgca accgactata tccgaatagg gtgcgcgcac gtcgaagtga ctcaacacga
    14881 aatctcgctc ctccaccgag ttgaaggcaa tcgccgtagg agactcgaac acccggcgaa
    14941 agattcgaaa gtgaatggcc ggttcgtcgt gcgccgtggg aatcagcagc gacttgcgcc
    15001 cagccaaagg caagcccgcg accgtgggat ggtagagata ggtgaagaac accagcacgt
    15061 cgaactcgcg agccgcgcgc tcgacgcgct cgagtaagcc ggagctcagc ggaccttgag
    15121 cg
//
LOCUS       DASTKU010000074        13486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_13884,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000074 DASTKU010000000
VERSION     DASTKU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13486
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13884"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="VFQ61_02490"
     CDS             complement(<1..1093)
                     /locus_tag="VFQ61_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953340.1"
                     /translation="MRTNILILGGLLLALQACGGSASGSIKTLSPDKVGGKDAKGNAV
                     SKEAAANFDQALQAFLEHDKKQDWAEGTCTSVAKMFEDAGSTQKSDTGRAFPEALYNA
                     GLAYQRCGKDEEARAQFQAANSADAGFHRAAAQMALYDYQRSGDIDGTISKLDQIIRD
                     AKFQNAEALVSLAALQMERGSDQSDQDGKDDLERAQKNLQRALAVDDTYMPAFNQLAA
                     YYLENAKRKSGAAKERTRRGKRGMEVSGAKRTEVNEQQLDLAALVASQAQRKNPNYAA
                     IHNTTGLIQVELKNFNSAVKSFGRARQLDPKFFEAHMNYAAVNLSFRGYQEAEKAYRE
                     ALKLRPKEYEAYLGLALAIRGQVNDGNFEK"
     gene            complement(1162..4926)
                     /locus_tag="VFQ61_02495"
     CDS             complement(1162..4926)
                     /locus_tag="VFQ61_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9953341.1"
                     /translation="MSSNRALSALAVAASMIVSQGSAWAASESKSSDLSAECIGTEAP
                     KALTTCPGGPSKFEANQKHAAAFRSAPPPQEAKKRQDDAKPVNPDELKKYAERDTRKT
                     RLQARARQLLTAEISGLERLYAQTKKNSPDRPQLIRRLAEGYVELESAAQRDKIAADI
                     KAQDAKKNKQDAGKFRSEASQAGKIVEAARGKAISYYTKMKNEYPTYSKLDEVLYYLA
                     YEHEQAGDYQNARSVYFELIQKAPKSTYIPNAYLAFGELFFQEAQGDPSKWDLAAAAY
                     QEVIKYPPPNNKVYGYARYKLGYVHWNKGEYAPTLSEFKKVIEYGEQYKDLPNAAQLA
                     KAARRDLIPVYAVSGAPDKAFNFFRPLSGDPGSETTKTIAMLNDLGLNYLDTGHYKEA
                     IVLYKDLLSRDSGNRSCFYQGQITTATQAVFSGDKEAVKKELEQQLRVRADFVKASHP
                     DKDKLECSNKTAELVAETAMSWHLEAVGSGGVRGTGDKKTMDLAAYLYKKVTENFTAQ
                     DFAKFQFPRIVKEDWPTMYKIRYAMADLLYFQQRWEECGPAFDSVVDEDPKAPEASEA
                     AYASVLCYQKMYDQLYKGESDRKGKGLGPTGDDKEREGKKGEWEKFKPKPFTDMQKGM
                     VQAFNRYVCYVKPPKGEKEAEEQYVEVKYARARTYFEAQHWEEAAMAFRDVALNHADK
                     DSGIFAAQLYLESVNVLGSRAEPPRPACFDDMARDVPQFLELYCKGQKLEDNKEQCEL
                     LTRIQFDIQRLKAQKIVELADSQAEKGNVKESNDNYRKGAESYLELWRGYCEEPLSKG
                     EKPKQCEKANEIVYNMARAYQAARLLAKSIQARQILLNPKYGMNESDLAMKATYEIGG
                     NYQAIAVYDRAAEWFERYAKATNYRGEFADQALSDASVLRLGLGQEDEAIADAANFNK
                     YFGAKKPTQTAQIAFAVAAHYGEKKDWANVEKRLGSSMRLIDSKATLDVRLQAHTLLA
                     RAYTKTNKGATAEKEYGAVTRMWADPKAGVGQIEAIEGEDANAKQRRLGRALEAVGEA
                     VFYFAEQKKAKVDKVKFPEYKGAGTKEAVVKHIQTKVKDWIGKKRPLIEDATQDYKKI
                     VDLQPVPPPRWVIAAGSRVGEMWGGFVKEFRAAPIPDMIKKDYELRTAYYGALDDASE
                     PQKQVAKSAYSTCLDYSVKYQYFDEFSRACEEWLAQNYKSEYHLVDEFRGSPNRMNSV
                     LREQGNPLRIGGEPIAAVVQDAGPAPKKKTEEKANADEKASKDKGGKKAK"
     gene            complement(4931..6685)
                     /locus_tag="VFQ61_02500"
     CDS             complement(4931..6685)
                     /locus_tag="VFQ61_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953342.1"
                     /translation="MFRKRLAVCMLAALAIPAVHTVTRSARAEDVIDLDEDPAAKKPA
                     KKATGNAGGKKKGVDIDLDEGGAAAPAAVTAGQMTEAAAAAKQLFDKERWNEAAQALQ
                     RVVSGETGDDPGNKQLAEYYLAISLYRLKFYQASYALFSVVADNPNHLKFKETLLWLA
                     KLATQLPEPADIIERVGKYTDDQVSRFNNEQQRDLYWQLNYMLGRYRYRNRQYNDAIR
                     LFQLVDRRSEYYVKAQFFTGISYVQVRKSVPAVKAFQRIIEALDEGVEGVEDEARMRD
                     LANLSIARTYYSASIKLDAETNAPNVDERHLSAAVKYWNSVDQASEYWLDALFEESWA
                     FYMAGRYPEALGNIHTIQSPYFPVAFYPEADILKAVIYFFNCNYDAAVTTVARFNKKN
                     TPIKEELEKVLKKYSGENQEEAFFKFLLQVREGNANLDPKIKPIVEAALSDRQLLRNI
                     EYVKLLDEEDKRFRKSPPSFQSSGIGQQVADALKLARDLAVRQAGELAMQRYRRNVEA
                     LAEHLRNGEKILIDVTAAQRNILDQKIAENKVTAAESKIYGVVKPDEEHVMWPFRGEY
                     WRDELGFYRQVVQSACGR"
     gene            7842..8534
                     /locus_tag="VFQ61_02505"
     CDS             7842..8534
                     /locus_tag="VFQ61_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953343.1"
                     /translation="MAAPSLLVMGLMIRISSLFAGSSSQGSGGLSRIRRIAAVSTALV
                     ALFALGSTMTGCETGGVGDPCTPEDEYLSDFAGFSIEEVNVESRSFQCETRVCLVNHF
                     QGRVKCPYGNAEGTPGCVIPGTSKSDPQNVVSASVDPQLKARPANNAVYCSCRCAGPE
                     KNAKYCECPSGFTCANLVDDYKIPNSAGQLAGSYCVREGTEYQKGAYAGVGNCKFNER
                     NCGEVSETGEPK"
     gene            8722..9192
                     /locus_tag="VFQ61_02510"
     CDS             8722..9192
                     /locus_tag="VFQ61_02510"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET9953344.1"
                     /translation="MGKRVATKDLGKLADSAAELGQLRHAKANPELNLQRQRERSAAG
                     KAAELAVVDWLVERGYTIVARNVRMGRYEIDIVAQLGPVIAVVEVRFRGTRAWTSGFG
                     SIDPAKRLRIRRAGERLWSRHYRNNPAVERLRFDAASVHFSGDQAEVTYVIAAF"
     gene            complement(9230..10021)
                     /locus_tag="VFQ61_02515"
     CDS             complement(9230..10021)
                     /locus_tag="VFQ61_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET9953345.1"
                     /translation="MTLETRSSFPAPGRGQRRLRNYLLDRRFQLKYAGYLVGIALLLS
                     AALGSVLWKTSQAALAQSQNAVRQGEEVVTRGRQVLAESQKVSAVVQMNIIRDPDYSQ
                     NPALLAAFRSDAEQQDARLAAQQRALEQQSQALKAQARDLGNQQRTVFLTLSGALGLL
                     VVLLGFAGIVVTHRVAGPIYKIKRQIREVGRGKLQIPDKLRRGDELVDFFETFEGMVK
                     NLRRRQEQEIVQLDAALAKLEGRVQESELAPLRSLRMDKQAALDA"
     gene            complement(10118..10957)
                     /locus_tag="VFQ61_02520"
     CDS             complement(10118..10957)
                     /locus_tag="VFQ61_02520"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9953346.1"
                     /translation="MTARHSNFICRSSARRVASRIGRILGLCVLLGSGISTAEPEGLP
                     RLLENLKHTDFRVRTQAALALGASKDDKARKPLCAALEDPNVSVRAASAAALGRLGSG
                     SEECLERRLATESDASAKAAIETSLEAIGGGEPTFTAQTRFYVAIGKVADKSGRSGGN
                     VDRQVRVTMQKTGKGVDGFAVAPLYETPAKAKSRMAKHAGVRGFYLSPRVPPFEYTGG
                     ALTVRLEIAMFSYPDKAMIGNYSVRLTQPDVPAPDVTSENELVAMAAERAVEKFARIA
                     PTL"
     gene            11109..12800
                     /locus_tag="VFQ61_02525"
     CDS             11109..12800
                     /locus_tag="VFQ61_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953347.1"
                     /translation="MKFGTLCRTLGSRVTLTATLLTAPVSRAADVNIRVHGQARVDAT
                     ARSFGNTTEVSGSLMDERGRAVVGRVSVRKLTATGRARTLSQARPCSPSEDLPAPEPS
                     ESADEARVRTDGKGRFCLQLTDVVGEGQLQVSFDEPKGWLERTAVSVEVARTAPLKLA
                     FRALPSRVAAESADFSVIVEARAETEGLTARPATLGLARSSNAFEVLERATLIPGQDL
                     HFTVNPKRLGEPGAIELVAESEVDGERVRARAPMLVTGRATVKVLEVPTADSQGESSV
                     SVGVTSSSGPITTGSVEARVGGRTVGIAAVAQGRARVLLALGATPGPVRVGLRYLPAS
                     PYWVGGEEQEIVLTVPEPSVWRKVPGLLSGCLIALWILAAWRRPPRREVPTPAREPVP
                     KLEWLPGPPGAQGFAGSILDAHDGTPIVGAQVSVFGPSISAQHATSDAEGHFELEPPS
                     DPRGVRIRVESNFHRALEQPLPRPGRLSIALVTRRRALLAQFVEWCERVGLSFPGQGE
                     PTPAELARRAQENQQPELARWADAVETAAFGPSAVDADYERRVELYKPSHRTPGE"
     gene            13149..>13486
                     /locus_tag="VFQ61_02530"
     CDS             13149..>13486
                     /locus_tag="VFQ61_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953348.1"
                     /translation="MDLVYILIALILLVGVFFLFTKKSDREELPPPRPERPSKRPKAK
                     VRSTRPAADRTEPEDQKQGDELQKRKPDAAKPSGTEAAQRGDRSTDESAEEIEAEVAA
                     PSGVEPSAPPP"
BASE COUNT         2377 a         4271 c         4122 g         2716 t
ORIGIN      
        1 ctttttcgaa gttgccgtcg ttcacctgcc cacgaatcgc gagagccaga ccgaggtaag
       61 cctcgtactc cttgggccga agtttgagcg cttcgcgata ggccttctcc gcttcttgat
      121 aaccgcggaa agagaggttc accgccgcat agttcatatg cgcctcgaag aacttgggat
      181 cgagctgacg agcccggccg aagctcttca ccgcgctatt gaagttcttc agctcgacct
      241 ggatcaaacc cgtggtgttg tggatcgcgg catagttcgg gttcttgcgc tgcgcttgtg
      301 aggccacgag tgcggcgaga tcgagctgct gctcgttcac ctcggttcgc ttggcacccg
      361 aaacttccat tccgcgtttg ccgcgacgcg tgcgttcctt ggcggcaccg gacttacgtt
      421 tggcgttctc cagataatag gccgccagct ggttgaaggc cggcatgtac gtatcgtcca
      481 cggccagcgc gcgctgcagg tttttctgcg cacgctccag gtcatccttg ccgtcctgat
      541 cggactggtc gctgccgcgc tccatttgga gcgccgcaag gctgacgagc gcctcggcgt
      601 tctggaactt cgcgtctcga atgatttgat cgagcttcga gatcgtgccg tcgatgtcgc
      661 ccgagcgctg atagtcgtac agagccattt gcgcggcggc ccgatggaag ccggcgtccg
      721 cgctgttggc ggcctgaaac tgcgcacgcg cttcttcgtc tttaccgcag cgctggtagg
      781 caagcccggc gttgtaaagc gcctcgggga atgcccggcc ggtgtccgac ttctgcgtcg
      841 aacccgcatc ctcgaacatc ttcgcaacgc tcgtgcaggt tccctcggcc caatcctgct
      901 tcttgtcgtg ctcgaggaag gcctggagtg cttgatcgaa gttggcagcg gcttccttgc
      961 tcaccgcgtt gcctttggcg tctttaccgc cgaccttgtc cggagacagg gtcttgatac
     1021 taccggaggc agagccgccg caggcttgca gagccagcaa gaggccgccg agaatcagaa
     1081 tgttggtccg catatcatcc gctccttaag cagaggctgg gcgtgccgcc cttccaaggg
     1141 tcggcacgcc cggggcgctc actacttggc cttcttgccg cccttgtcct tggacgcttt
     1201 ctcgtccgcg ttggccttct cctcggtctt cttcttcgga gctggcccgg catcctgcac
     1261 caccgccgcg atcggctcgc caccgatacg aagcggatta ccttgctcac gcagcacgct
     1321 gttcatccgg ttgggtgaac cgcggaactc gtcgaccaag tggtactcgg acttgtagtt
     1381 ctgggcgagc cactcctcgc atgcacggct gaactcgtcg aagtactggt acttgaccga
     1441 gtagtccaag caagtggagt aggccgattt ggcaacctgc ttctggggct ccgaggcatc
     1501 gtcgagagcg ccgtagtacg ccgtacgcag ctcgtagtcc ttcttgatca tgtcggggat
     1561 cggcgcggcg cggaattcct tgacgaaccc gccccacatc tctcccactc gcgagcccgc
     1621 tgcgataacc caacgcggcg ggggcaccgg ctgcagatcg acgatcttct tgtagtcctg
     1681 cgttgcgtcc tcgatcagcg ggcgcttctt gccaatccaa tccttgacct tggtctggat
     1741 gtgcttgacg accgcttctt tggtgccggc tcccttgtac tcggggaact tcaccttatc
     1801 gaccttcgcc ttcttctgtt cggcgaagta gaagaccgct tcgccgacgg cttcgagcgc
     1861 gcgacccaag cggcgttgct tggcgttggc gtcttcacct tcgatcgcct cgatctggcc
     1921 gacacccgcc ttcggatccg cccacatcct ggtcaccgcg ccgtattcct tctcggcggt
     1981 cgcgcccttg ttggtcttgg tgtaggcgcg ggcaagcaac gtgtgcgcct gcaagcgcac
     2041 gtccaacgtc gccttggagt cgatcaggcg catggagcta cccaggcgtt tctcgacgtt
     2101 cgcccaatcc ttcttctcac cgtagtgagc ggcgacagcg aatgcgatct gcgcggtctg
     2161 cgtcggcttc ttggcaccga agtacttgtt gaagttggcg gcgtcggcga tggcttcgtc
     2221 ctcttgcccc agaccgagac gcaacaccga ggcgtcgctc agggcctggt ccgcgaactc
     2281 accacggtaa ttcgtggcct tcgcgtagcg ctcgaaccac tcggcagcgc ggtcgtaaac
     2341 cgcgattgct tggtagttgc cgccaatttc gtacgtcgcc ttcatcgcca aatccgactc
     2401 gttcatgccg tacttgggat tcagcaggat ctgacgcgct tgaatcgatt tcgcgagcag
     2461 gcgcgccgcc tggtaggcgc gagccatgtt gtagacgatt tcgttcgcct tctcgcactg
     2521 cttgggcttc tcacccttgc tgagcggctc ctcgcagtag ccacgccaaa gctcgaggta
     2581 cgactcggct cccttgcggt agttgtcgtt gctctctttg acgttgccct tctctgcttg
     2641 agagtccgcc agctccacga tcttctgcgc tttcaagcgc tgaatatcga actggattcg
     2701 ggtgagcagc tcgcactgct ccttgttgtc ttcgagcttc tgacccttgc agtacagctc
     2761 gaggaactgc ggcacgtcgc gggccatgtc gtcgaagcag gccgggcgcg gcggctcggc
     2821 gcgcgagccg agcacgttga ccgactccag gtacagctga gcggcgaaaa tacccgagtc
     2881 tttgtcagcg tgattgagcg cgacgtcgcg gaatgccatc gccgcctcct cccagtgctg
     2941 agcctcgaag taggtacgag cgcgcgcgta cttcacttcc acgtactgct cctccgcctc
     3001 cttctcaccc ttgggcggct tcacgtagca gacgtagcga ttgaaggcct ggaccatgcc
     3061 cttctgcatg tccgtgaagg gcttgggctt gaacttctcc cactccccct tcttgccttc
     3121 gcgctccttg tcgtcgccag tggggccgag acctttacct ttgcggtcgg actcgccctt
     3181 gtacagctgg tcgtacatct tctggtagca gagcaccgaa gcgtacgcgg cttccgaggc
     3241 ctcgggcgcc ttgggatcct catcgacgac cgagtcgaat gccggaccgc actcctccca
     3301 gcgctgctgg aagtacaaca agtccgccat cgcgtagcgg atcttgtaca tcgtcggcca
     3361 gtcttccttg acgatgcgcg ggaactggaa cttcgcgaag tcctgggccg tgaagttctc
     3421 cgtcaccttc ttgtaaaggt aagcggccag gtccatcgtc ttcttgtcgc cggtgccgcg
     3481 cacgccgccc gagcccaccg cctccaagtg ccagctcatc gccgtttccg cgactagctc
     3541 ggcggtcttg ttggagcact cgagcttgtc cttgtccgga tggctcgctt tgacgaaatc
     3601 cgcacgcacg cgcagctgtt gctcgagctc tttcttgacg gcttccttgt cgccagagaa
     3661 cacggcctgg gtcgccgtcg tgatctgacc ttggtagaag cacgagcggt tgccggagtc
     3721 gcgagagagc aggtccttgt agaggacgat ggcttccttg tagtgaccgg tatccaggta
     3781 gttgagaccc agatcgttca acatcgcgat cgtcttggtc gtctcactgc cgggatcacc
     3841 cgagagcggg cggaagaagt tgaacgcctt gtccggcgca ccactcaccg cgtagacggg
     3901 gatcaagtcg cgacgcgctg ccttcgccag ctgagccgca ttgggcagat ccttgtactg
     3961 ctcgccgtac tcgatgacct tcttgaactc actcaaggtc ggcgcgtact cgcccttgtt
     4021 ccagtgcacg tagccgagtt tgtaacgcgc gtagccgtac accttgttgt tcggcggcgg
     4081 gtacttgatc acctcttggt acgcggccgc ggccaagtcc cacttggaag gatcgccctg
     4141 ggcttcctgg aaaaacagct cgccgaaggc caggtacgcg ttcgggatgt acgtcgactt
     4201 cggagccttc tgaatcagct cgaagtaaac cgagcgcgcg ttctggtagt cacccgcctg
     4261 ctcgtgctcg tacgcgaggt aatacagcac ctcgtcgagc ttcgagtacg tcgggtactc
     4321 gttcttcatc ttcgtgtagt acgagatggc tttgccgcgc gcggcctcga cgatcttgcc
     4381 ggcttgtgac gcctcgctgc ggaactttcc ggcgtcctgc ttgttcttct tggcgtcttg
     4441 agccttgatg tcggccgcga ttttatcgcg ctgtgctgcc gactcgagct ccacgtaacc
     4501 ttcagccaaa cgccgaatca gctgcggacg atcgggcgag ttcttcttgg tctgagcgta
     4561 caaacgctcg aggcccgaga tctccgccgt cagcagctga cgcgcgcgag cctgtaggcg
     4621 cgtcttgcgc gtatcgcgct ccgcatactt cttcagctcg tctgggttga ccggcttggc
     4681 gtcgtcctga cgcttctttg cctcctgggg tggaggcgca gagcggaaag ccgccgcgtg
     4741 cttctggttg gcctcgaact tcgaagggcc accagggcag gtcgtaaggg ctttgggagc
     4801 ttcggtgccg atacactcgg cgctcaaatc agacgacttc gattcggacg cggcccatgc
     4861 ggagccttgg gacacaatca tgctcgcggc gaccgcgagc gcggacaggg ctctattcga
     4921 gctcatcaca ttacctccca caggcagatt gaacgacctg ccgatagaaa ccgagctcgt
     4981 cacgccagta ctcgccccgg aagggccaca tcacgtgttc ttcgtctggc ttgacgacgc
     5041 cgtagatctt ggattcagcc gcggtgacct tgttctccgc gatcttctgg tcgaggatgt
     5101 tgcgttgcgc ggccgtaacg tcgatcagga tcttctcacc gttgcgcaaa tgctcggcaa
     5161 gagcctctac gttccggcga tagcgctgca tagcgagctc gcccgcctga cgcaccgcta
     5221 gatcacgcgc caacttcaga gcgtcggcca cttgctgacc gatgcccgag ctctggaagc
     5281 tcggcggtga cttgcggaac cgcttgtcct cttcgtcgag caacttcacg tactcgatgt
     5341 tgcggagcag ctgccggtcg ctgagcgcgg cctccacgat cggcttgatc ttggggtcga
     5401 ggttggcgtt gccctcgcgc acctgcagca ggaacttgaa gaacgcctct tcctggttct
     5461 cgcccgagta cttcttcagc accttctcga gctcctcctt gatgggcgtg ttcttcttgt
     5521 tgaagcgggc gacggtggtc acggctgcat cgtagttgca gttgaagaag tagatgaccg
     5581 ccttgagaat gtccgcttcc ggatagaacg cgaccgggaa gtacggcgac tggatggtgt
     5641 ggatgttgcc gagcgcttcg ggatagcggc cggccatgta gaacgcccac gactcctcga
     5701 acagcgcgtc gagccagtac tcactcgcct ggtccaccga gttccagtac ttcaccgcgg
     5761 ccgacaagtg acgctcgtcc acgttcggcg cgttcgtctc cgcgtcgagc ttgatcgagg
     5821 cggagtaata ggtcctcgcg atcgacaggt tcgcgaggtc gcgcatgcgc gcctcgtcct
     5881 cgacgccctc cacgccctcg tccaacgcct cgatgatgcg ctggaaggct ttgaccgcgg
     5941 gcaccgactt gcgcacctgc acgtagctga tgcccgtgaa gaactgcgcc ttgacgtagt
     6001 actcgctgcg ccgatccacg agctggaaca gacgaatggc atcgttgtac tgccggttcc
     6061 ggtagcgata ccgaccgagc atgtagttca gctgccaata cagatcgcgc tgctgttcgt
     6121 tgttgaagcg gctgacctga tcgtcggtgt acttaccgac gcgctcgatg atgtccgccg
     6181 gctccgggag ctgcgtcgcc agcttggcta gccacagcag cgtctccttg aacttcaggt
     6241 gattcgggtt atccgcgact acgctgaaga gggcgtaact cgcctgatag aacttcaaac
     6301 ggtaaagaga gatcgcgagg tagtactcgg cgagctgctt gttacccggg tcgtcacccg
     6361 tctccccact gacgacgcgc tgcagcgcct gagcggcttc gttccagcgc tccttgtcga
     6421 agagctgctt ggcggctgca gccgcttcgg tcatctgccc ggcagtgacc gcggcgggcg
     6481 ccgctgcgcc tccctcgtcg aggtcgatat cgaccccctt cttcttgccc cctgcattgc
     6541 cagtcgcctt cttcgcaggc tttttcgcag cagggtcttc gtccagatcg atgacgtctt
     6601 ccgcgcgcgc ggagcgggtc acggtgtgaa ccgcaggaat ggccagagct gccaacatgc
     6661 aaacggcgag gcgctttcga aacatgaatc ctctcttcac gacgttgcct cggtcaggga
     6721 gcaacgccgc gttgtggccg gaaacttacg agcccagggg aacggaaaac caaaattcgt
     6781 aataactacc gctggatacc ctaccgcggc cgccgggcat gttagatacc gtgccagagg
     6841 cttgtcaagc gccgttcgga ccgcgttaca gggatttttg gaacctgctg tgtgccgcga
     6901 aatttcgcgt ttcacatgat tgagccggat cgcgtcaaac ctcccagtga cctcaatcag
     6961 gcaccggccg attgaaggat gagctgcaca tggcgaagca tcgaagaggc tagagcaact
     7021 tggaaccgac taaccgagct cgaaccgcag tgggccgacc caacggaaac ggcgcaaagc
     7081 caagactaaa gaaactggcg aaacgaacca gcgtgacaga acgagccggg gcgcacctta
     7141 cggccggcgc taatccagag caacatggac cagcgacttc gatcaaagcc tggcgcgtcc
     7201 gtcagcacct tggccttaga agcgcaaccg tgtccggcag aggggacgaa tcggttagcc
     7261 gaaagcgaag ttaggtcagc ccgacggaag cgaagaacag gagccgaggc gtgaccagag
     7321 atcctgtcgg cgaacgagag acagcgaggg gagatagagt gaaggagaga gagcaaagga
     7381 gatggaccga ggagaagtgg gccggcgagc agagcccggg agaggctaga taggatctga
     7441 gtcgggttgg cgcggattcg ggtgacctcg atcaacgggc ggctgtatct ataacgattt
     7501 tgccggcacg ttagccggtc ctgctcgcgc gcctggcgca agcatgcgaa gattccggac
     7561 gcacggcgtg gtccgcgctg accgcgccgc acgagaaccc gagaagcccg agaagccgtg
     7621 ccgcccgaga agcatgtagc catgcatgcg caatgtctcc gacggttggc cctcgagcgc
     7681 cgcgaagcat gggacacgct agttcgaaat ttccttggga ccctccgggc caagtgcgaa
     7741 caacaaaaac cgccggtcat gagccggaag ttcgcgtgct ttccacattg acgtagttcg
     7801 ctgccgtcga ctagcgtacc gccggtcggt ttggccgcga attggctgct ccttccctgt
     7861 tggtgatggg cctaatgatc cgaatctcct ctctgtttgc tggtagttct tcccaaggta
     7921 gcggcggtct ttccagaatc cgtcgtatcg cggccgtatc caccgcactg gtggcgctgt
     7981 tcgccctcgg cagcaccatg accggttgcg agacgggcgg agtgggtgac ccttgcactc
     8041 ccgaggatga gtacctcagc gacttcgccg gattcagcat cgaggaagtg aacgtcgaga
     8101 gccgctcctt ccagtgcgaa acccgcgtct gcctggtgaa ccacttccag gggcgcgtga
     8161 agtgccccta cggcaacgcg gaaggtaccc ccggctgcgt gattcccggc accagcaaga
     8221 gcgatccgca aaacgtagtc agcgcgtccg tcgatccgca gctcaaagcg cgaccggcga
     8281 acaacgccgt ttattgctcc tgtcgctgcg cgggcccgga gaagaacgcc aagtactgcg
     8341 agtgtccatc gggctttacc tgcgcgaacc tcgtggatga ttacaagatt ccgaacagcg
     8401 cgggtcagtt ggcgggctcg tactgtgtcc gtgagggaac cgagtaccag aagggcgcgt
     8461 acgctggcgt gggtaactgc aagttcaacg aacggaactg cggcgaagtc agcgaaacgg
     8521 gcgaacctaa gtaaggtcca tgctcgacgc accctctgcg gtgcccggcc ggcggtgagt
     8581 cgcgatgtgc ggcgagaccg ccggttcgct tttgtacggt cgcaaactga cgtagaaccg
     8641 agttaccacg gagtcgttcc tgcgccttca cttgcagcca accccggcac ttcttagagt
     8701 cgtacggaaa ggggtgagcg catggggaag cgggtcgcaa ccaaggatct gggcaaactt
     8761 gccgattcgg ctgccgagct cggccagctt aggcacgcga aggccaatcc tgaactcaat
     8821 ctacagcgtc agagagaacg gagcgcggcc ggaaaagccg cggagttggc cgtcgtcgat
     8881 tggctcgtcg aacgcggcta tacgattgtc gcgagaaacg tgcgaatggg ccgctacgag
     8941 atcgatatcg tcgcgcagct cggcccagtc atcgccgtcg tcgaggttcg ctttcggggc
     9001 acccgcgcgt ggacgagcgg gtttggcagc atcgatcccg ccaagcgtct gcgcatccgc
     9061 cgcgcggggg agcggctctg gtcacgacac tatcgcaaca atcccgcggt cgagcgactt
     9121 cgcttcgatg ccgccagcgt ccacttctcc ggagaccagg ccgaagtgac gtacgtcatc
     9181 gccgcttttt gagcgtcgca gcctgcaagt tgtcagccgt cgacaaactt caggcgtcca
     9241 gcgcggcctg cttatccatt cggaggctgc ggagcggagc caactcggat tcctgcacgc
     9301 gcccctcgag tttcgcgagt gccgcgtcga gctgcacaat ctcctgttcc tgccggcgcc
     9361 ggagattttt caccatgccc tcgaacgtct cgaagaagtc cacgagctca tcccctcgcc
     9421 tcagcttgtc cgggatctga agctttcctc gcccgacctc ccgaatctgt cgtttgatct
     9481 tgtaaatcgg ccccgccacg cgatgcgtta cgacgatgcc cgcgaagccc aggagcacca
     9541 cgagcaagcc gagagctccc gagagcgtca agaacacggt gcgctgctga ttgccgagat
     9601 cgcgagcttg cgccttcaac gcctgagact gttgctcgag cgcccgctgt tgagctgcaa
     9661 ggcgcgcatc ttgctgttca gcgtcggaac gaaaagccgc gagcagcgcc gggttctggc
     9721 tgtagtcggg atcgcggatg atgttcatct gcacgaccgc gctcactttc tggctctcag
     9781 ccagaacctg gcgaccccga gtaaccacct cctcgccttg ccgcaccgcg ttttggctct
     9841 gcgccagcgc cgcctggctg gtcttccaaa gcaccgagcc cagcgcggcg ctcagcagca
     9901 aagctatccc cacgaggtag ccggcgtatt tgagctggaa gcgacgatcc agcaggtaat
     9961 tcctgaggcg acgttgaccg cgaccaggcg ctgggaacga agaacgagtt tcgagggtca
    10021 tgggaccgac gctccgaatg cccgttgcga gcgggctggc tcatttaaga ctgacagcaa
    10081 aactagcctg tactcgaggc ctgcgcgaat catccgacta caaggtaggc gcgatgcgcg
    10141 cgaacttctc gaccgcgcgc tccgcggcca tagcaacgag ctcgttttcg ctcgtcacgt
    10201 ctggcgcggg cacgtcgggc tgcgtgaggc gcacactgta gttaccgatc atggccttgt
    10261 cgggatagct gaacatcgca atctcgagcc gaaccgtgag ggctccgccg gtgtactcga
    10321 aggggggtac tcgaggggac aagtagaagc ctcgcactcc ggcgtgcttg gccatacgag
    10381 acttggcttt ggccggtgtc tcatacaacg gcgccacggc gaatccgtct acccccttgc
    10441 ctgtcttctg catcgtgacc cgcacttgcc ggtcgacgtt gccgccgctg cgtccgctct
    10501 tgtccgcgac tttgccgatt gcgacgtaga agcgggtctg agcggtaaag gtcggctcgc
    10561 cacctccgat tgcctcgagc gaggtttcaa tggcggcttt ggccgaggcg tccgactctg
    10621 tcgccagtcg gcgctccagg cattcttcgc tgccgctgcc caggcgtccc aaagccgccg
    10681 cgctggcggc tcgcacgctc acgttgggat cctccaaggc tgcgcagagc ggcttgcgag
    10741 ccttgtcgtc cttggacgct cccaacgcca acgcagcctg ggtacgtact cggaagtccg
    10801 tatgcttcag gttttcgagc agacggggta gcccctcggg ctcggcggtg gagatgccgc
    10861 taccgagcag cacacaaaga cccaaaatcc ggccaatacg gctggcgact cgtcgtgcac
    10921 tcgaacggca aatgaaattg gaatgacgtg cggtcaaaga ccttctcgcg ggctcgtcgt
    10981 tattgacggt cgttctacgg cgcggcttag ctggctattt cccccggcaa atggctttcg
    11041 aagctgaatc ttcgcactta ctcgcgtgtc ctttggccga gagggagcca cgaggtaggc
    11101 ttggccgcgt gaagttcggc actttatgcc gcaccttggg cagccgggtg acgctgacag
    11161 ccaccctttt aaccgccccg gtcagtcggg ccgcggacgt aaacattcgg gtacatggac
    11221 aggcccgggt cgatgcaacg gcacgcagct tcgggaatac caccgaggtc tcgggctcgc
    11281 tcatggatga gcgcgggcgc gcggtcgtcg gtcgagtgag tgtgcgtaag ctgaccgcga
    11341 cgggccgggc tcgaacgctt tcgcaggcgc ggccctgttc tccctcggag gaccttcccg
    11401 cacccgagcc aagtgaatcc gcagacgaag cgcgggtgcg caccgatggc aaggggcgct
    11461 tctgtctgca gctcacggac gtggttggtg aggggcaact gcaagtttcg ttcgatgaac
    11521 cgaaaggttg gctggagcgc actgccgtca gcgtggaagt ggcgcgaacc gcgcccctga
    11581 agctcgcgtt tcgtgcactg ccgagccggg tcgcggcgga atccgcggat ttctccgtca
    11641 tcgtggaggc tcgcgccgaa actgagggct tgaccgcgcg ccccgctacc ctgggtttgg
    11701 cgcgatcctc caacgccttc gaagttttgg aacgggcgac gctcattccc ggacaagacc
    11761 ttcacttcac cgtgaacccc aaacgcttgg gcgaacctgg cgcaatcgaa ctggtcgcgg
    11821 agagcgaagt ggacggcgag cgagtacgcg ccagagcccc catgctcgtg accggtcgcg
    11881 ccaccgtcaa ggttttggag gtgccaaccg ccgatagtca gggagagtcg agcgttagcg
    11941 ttggcgtgac ctcttcttct ggcccgatca ccacgggatc agtggaggcg cgcgtaggcg
    12001 gacgcaccgt tggcatcgcg gccgttgctc aagggcgcgc ccgcgtttta ctggccctcg
    12061 gcgctacacc cgggcccgtt cgcgtcggct tgcgctactt gcccgcaagc ccctactggg
    12121 tcggcggcga ggagcaggaa atcgtgctca cggtgcccga gccgagcgtc tggcgaaagg
    12181 tgcccggcct actctcgggg tgcttgatcg cactttggat cctggccgct tggcggaggc
    12241 caccgcgtcg cgaggtgccc acgcccgccc gcgagcccgt cccgaagctc gaatggctgc
    12301 ccggaccgcc cggagcccaa ggcttcgcgg gtagcatcct agatgcgcac gacggaacac
    12361 cgattgtcgg cgcccaggtc tccgtgttcg ggccaagtat ctccgcgcaa cacgcgacga
    12421 gcgacgcgga aggtcacttc gagttggagc ccccgagtga ccctcgcgga gtccgcatcc
    12481 gcgtggagtc gaactttcac cgcgcactcg agcagccact accacgtccc ggccggctct
    12541 cgattgcgct cgtgacacgc cgacgggccc tcctcgcgca gttcgtcgag tggtgcgaac
    12601 gcgtgggtct gtcctttccc gggcagggcg aacccacccc cgccgaactg gcgcggcgag
    12661 cacaggagaa tcagcaaccg gagctggcgc gttgggcgga tgccgtggaa acagccgcat
    12721 tcggcccctc tgcggtagac gccgactacg agcgccgggt cgagctctac aagccgagtc
    12781 accgtacccc gggtgaatga acccgtgtcg tctgcgtgct cctggtctgc tccgccaggc
    12841 aggccgcccg cccgcccgtt gccaagctcg aaagcgaggc aatcgaccct acctttgctc
    12901 ccaaagctgc ttttgacggt catcgagcgg cgctcacccg gagtcgtagt ccccggttac
    12961 ccagttaccc gaaccttgca gcccccgcac gttggggctc gccactcatg gttgcgaatg
    13021 ccgcactgcc caacgacaga tgaatactgc ccaacggcac acgaatcgag cattgtcgga
    13081 acgcggaggt tgacggggct cgccgcaggt ttctatagtc gcgcggcctc gaactttgcg
    13141 ctgactcgat ggatctagtt tatatcctca tcgcgctcat cttgttggtc ggcgtgttct
    13201 tccttttcac gaagaagagc gaccgagagg agctaccgcc accccgaccc gaaaggccct
    13261 cgaagagacc caaggcgaag gttcgttcga cgcgacctgc ggcggatcgc accgagccgg
    13321 aagatcagaa gcagggcgat gagctccaga agcggaaacc cgacgcggca aagcccagcg
    13381 gtaccgaagc tgcgcaacga ggtgaccgga gcacggatga gtccgcggag gaaatcgaag
    13441 ccgaggtggc agcaccatcc ggtgtcgagc ccagcgcgcc cccacc
//
LOCUS       DASTKU010000075         4068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_235755, whole genome shotgun sequence.
ACCESSION   DASTKU010000075 DASTKU010000000
VERSION     DASTKU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235755"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFQ61_02535"
     CDS             complement(<1..546)
                     /locus_tag="VFQ61_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9953349.1"
                     /translation="MQKIRGSHVLITGAARGIGRAVALELATRGARLELVDLDESGLK
                     SVREEAVERGALAASVARVDVSDAAAVEDLARRVLARTGPLDALINAAGVAVFGPVLD
                     TDEELWRHVLEINLLGTVRCTRAFAPSMVERGRGQIVNVASASAFCAVPGLGAYATTK
                     YAVLGLSEALRSELAASGVGVS"
     gene            complement(580..2517)
                     /locus_tag="VFQ61_02540"
     CDS             complement(580..2517)
                     /locus_tag="VFQ61_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HET9953350.1"
                     /translation="MAVVPLRVARNSRPALPHSAFLPTTREEMQARGYDELDILIVTG
                     DAYVDHPAFGPVLIARFLEGRGYRVGVIAQPDWRNPEDISRMGRPRLFVGVSAGNLDS
                     MLNKLTAQKKVRSEDQYSPDGRTNCRPNRATIVYSNLCRQAFPGLPIVLGGIEASLRR
                     IAHYDYWSDSVRRSVLLDAKADLLVFGMGERAAWEIARRLDAGEPVSALQDVRGTAHV
                     KNNPEEWRTIVENPSKFVRDGKVVVLPSYEEVVADKRQFANMSRLFQLETNPHNGRPL
                     LQVHGEQAVYFNSPALPLEEGEMDGLYALPFARLPHPSYGGERIPAFETVKHSIVTMR
                     GCFGGCTFCSITEHEGRIIQSRSKESVLKEVRALSRMEGFSGVLSDVGGPTANMYKMH
                     CRSPEIESACRRLSCVHPGICENLVTDHTPLIDLLASVRREKGIKRTMIASGVRYDLA
                     ERSPEFIRDLAAHHTGGQLSVAPEHNNPEVLKRMKKPNIVSYERFAQAFCHESEKAGK
                     EQYLVPYFITGHPGSTIKDTIELALYLKRNDMRPRQIQDFIPTPMAVATTMYYTGIDP
                     LHNEPVPVVRELREKRQMKALILWWDEQQWPLAREALRKAGRFDLIGNAKHCLVPDEA
                     RGNRRSPPRPLGRPARARRTP"
     gene            2877..3869
                     /locus_tag="VFQ61_02545"
     CDS             2877..3869
                     /locus_tag="VFQ61_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HET9953351.1"
                     /translation="MTGAGCAASADSEGLAQVAGVGGSADSAQTAPASPVPGTESSGG
                     GSPGAGTGSGAAAGGFRGLAVQGGTGGASNNDASGASAGASIKAETSVYAAACGDGRL
                     LTGVVRDFDPLVHPDFEPTSKAIKSPDVRLSGMKRWTSELGIVRPMLGPDFKPVYSGS
                     ESGTQTTFGATWFQSWFRDTPGLNASAEIQIELVDPDGDGVFVFDNSGQPFFPIDHQL
                     LGNSAAEYGSELAAHNFGFTYELHAKFSYDDENMIIGVSGNDDLWVFVSGRLVIDLGG
                     LGSKSGKIRLRDLGLTLKREYQLDLFGAERAAPDSQFALESNVHFTSCALSVPR"
BASE COUNT          716 a         1346 c         1248 g          758 t
ORIGIN      
        1 cgacacacct accccgctcg cggccagttc gctgcgcaac gcctcggata gtccgagcac
       61 ggcgtacttg gtcgtcgcgt atgcgcccag cccgggcacg gcgcagaatg cagacgccga
      121 cgccacattc acgatctgac ctcggccgcg ctcgaccatg ctgggcgcga aagctcgcgt
      181 gcagcgcacg gtgccgagca ggttgatttc gagcacgtgc cgccacagct cctcgtccgt
      241 gtccaaaacc ggcccgaaca ccgccacgcc cgccgcgttg attaacgcat ccagcggacc
      301 cgtacgcgcg agcacccggc gcgcgagatc ctcgacggcg gccgcgtcac tcacgtccac
      361 gcgcgcgacg gatgccgcga gcgccccacg ctcgaccgct tcctctcgaa cactcttcag
      421 tccggattca tccagatcga cgagctcgag ccttgcgcct cgggtcgcga gctcgagcgc
      481 caccgcccga ccgatgccgc gcgcagcccc ggtaatgagc acgtgcgatc cacggatctt
      541 ttgcaccgac acccggctct ccgctctcga ttccgggaat cagggggtgc ggcgcgcccg
      601 cgcgggccgt cccagcgggc ggggcgggct cctgcgatta ccgcgcgctt cgtcgggaac
      661 gaggcaatgc ttggcattgc cgatcaaatc gaagcggccc gctttgcgaa gcgcttcccg
      721 cgcgagcggc cactgctgct cgtcccacca caggatcaag gctttcattt gccgcttttc
      781 acggagctcg cgcaccacgg ggaccggctc gttgtgcagc ggatcgatgc ccgtgtagta
      841 catggtcgtg gccaccgcca ttggcgtggg gatgaagtct tgaatttgac gcggccgcat
      901 gtcgttgcgc ttcaaataaa gcgcgagctc gatcgtgtcc ttgatcgtcg agccagggtg
      961 cccggtgatg aagtagggca cgagatactg ttctttccct gccttctcgc tctcgtggca
     1021 aaaggcctgg gcgaagcgct cgtagctcac gatgttgggc ttcttcatgc gcttgagcac
     1081 ttcggggttg ttgtgctccg gcgcgaccga aagctgacca cccgtgtggt gagcggcgag
     1141 atcgcgaatg aactcggggc ttcgctcggc cagatcgtag cgcacgcccg aggcgatcat
     1201 cgtgcgcttg atccccttct cgcggcgcac ggacgccagc aaatcgatga gcggcgtgtg
     1261 atccgtgacc agattctcac agatccctgg gtgcacgcag gagagccgac ggcacgcact
     1321 ctcgatctcg ggcgatcggc aatgcatctt gtacatgttg gcggtcgggc cgccgacgtc
     1381 actcaaaact ccgctgaagc cctccatgcg agacagcgcc cgaacttcct tcaagacgct
     1441 ctctttcgat cgactctgaa tgattcggcc ttcatgctcg gtgatgctgc agaaggtgca
     1501 acctccgaaa cagccgcgca tggtcacgat cgaatgcttg accgtctcga acgccgggat
     1561 ccgctcgccg ccgtagcttg ggtgcggcag ccgagcgaaa ggcaaagcat agagcccgtc
     1621 catctcgccc tcttcgagcg gcaacgccgg ggagttgaag tagacggcct gttccccgtg
     1681 cacctgtagc agcggacgcc cgttgtgggg gttggtctcc aactggaaga ggcgcgacat
     1741 gttcgcgaac tgccgcttgt cagccacgac ctcttcgtag ctcggcagca cgacgacctt
     1801 gccatcacgt acgaacttgc tcggattctc gacgatcgtg cgccactctt cggggttatt
     1861 ttttacgtgc gccgtgccgc gcacgtcctg cagcgccgat acgggctcgc ctgcgtccaa
     1921 gcgccgcgcg atctcccaag ccgcccgttc acccattccg aacacgagca ggtcggcctt
     1981 tgcgtccagc agcaccgagc gccgcaccga gtccgaccaa tagtcgtagt gcgcgatgcg
     2041 ccggagcgac gcttcgatgc cgcccagcac gatcggcaag cccggaaagg cctggcgaca
     2101 caggttcgag tacacaatcg ttgctcggtt cgggcggcag ttcgtgcgcc cgtccggtga
     2161 gtattgatcc tcggatcgca ccttcttttg ggccgtcagc ttgttcagca tcgagtccag
     2221 attcccggcg ctgacaccca cgaacaggcg cgggcgtccc atgcgcgaaa tgtcttccgg
     2281 attgcgccaa tccggttgag caatcacacc tacgcgataa cctcgcccct ccagaaaacg
     2341 cgcgatcagc accgggccga acgcgggatg atccacgtag gcgtcacccg tgacgatcag
     2401 gatgtcgagt tcgtcgtagc cacgcgcctg catttcctca cgagtggtcg ggaggaaggc
     2461 ggagtgcggc agcgcgggac gagagttgcg ggcgacgcgt agcggaacga cggccatgct
     2521 agggaccacc cactagcact agtttgtcag ctcagcgggg cggtgagccg ggtgccagag
     2581 ctcagcccca tctttcggga ggacaggtcg actcctgtgc gccggccgct gagggccatg
     2641 gcgatgcggg gccgcaaacg atggcccccc gctcctcttc gagctaggct gacccattcg
     2701 cgtcggcctc cgacgccttt cttacttggt tgcgcatgtc ttttcgtttg gacctattca
     2761 cgggtcgtgc tgaacgcgtt tcggcgtcaa agctctcaaa atcaaccaac tcaacgaacc
     2821 cggcgcaggg gcaccttgca ccgctcggcc ttctcgcgac cgccagcctt ctcggtttga
     2881 ctggggcagg atgcgccgca tccgctgact ccgaaggcct cgctcaggtc gccggggttg
     2941 gcggatccgc ggattccgcg cagaccgctc ccgctagtcc cgttccgggc accgagagct
     3001 caggcggagg ctctccgggc gctggaaccg gatcaggggc cgcggcgggt ggctttaggg
     3061 gtttggctgt tcaaggcggc acgggcggcg cttcgaacaa cgacgcgagc ggtgcgtccg
     3121 ccggagcttc gatcaaggcc gaaacgagcg tgtacgccgc cgcttgcggt gacggccgct
     3181 tgctgacggg cgtcgtcagg gacttcgatc cactagtcca ccccgatttc gagcccacga
     3241 gcaaagcgat caagagcccc gacgtgcgcc tctctgggat gaagcgatgg acgagcgagc
     3301 tcggcatcgt ccgtccgatg ttgggtcctg atttcaagcc cgtttactca ggcagcgagt
     3361 cgggcaccca gaccacgttc ggcgctacct ggtttcagag ctggtttcgt gacacgcccg
     3421 gtttgaacgc cagcgccgaa atccagatcg agctcgtcga tccggacggg gacggagtgt
     3481 tcgtgttcga caactcgggg caacccttct ttccgatcga ccaccagttg cttggtaatt
     3541 cggcggctga gtacggctcc gagctcgcgg ctcacaactt cggttttacc tacgagctac
     3601 acgccaagtt ctcgtacgac gacgagaaca tgataatcgg agtctccggc aacgacgacc
     3661 tctgggtctt cgtcagcggg cgcttggtca tcgacttggg cgggctcggc agcaaatcag
     3721 gcaaaattcg ccttcgcgat ctagggctca cgctgaaacg agagtatcag ctggatctat
     3781 tcggtgccga acgcgccgcg cctgattctc agttcgcact cgagagcaac gtgcacttca
     3841 cgagttgcgc gctttcggtg ccgcgctgac cttctcgcct ttgcagtcgg cctgacggtg
     3901 atcgaccgcg cagcgagcat ctaggcggcg gcgcacggga cgcaaccgaa cgcctattcc
     3961 agcgcgtcga agacctcgcg caagcgcact aaatcagtct cggcaacgta gccaccgaaa
     4021 ccaattctca tcgagatcgt ggtttcgtct ccccagggcc accacaag
//
LOCUS       DASTKU010000076         4011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_235843, whole genome shotgun sequence.
ACCESSION   DASTKU010000076 DASTKU010000000
VERSION     DASTKU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235843"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1084..2577)
                     /gene="csb2"
                     /locus_tag="VFQ61_02550"
     CDS             complement(1084..2577)
                     /gene="csb2"
                     /locus_tag="VFQ61_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HET9953352.1"
                     /translation="MIGLELRFLAGRYHGTGWHSAHNEAVPEWPPSPWRILRSLVSAA
                     YSEGAPQPAVTSLLGKLRDLPKYRLPRAVEAHTRHYMPDTADAEHKRSKVFDAFVAIQ
                     GGAGDPEPLIIAWPGELTPDERALLQQLCRRITYLGRAESWVEVRPSEKPNDHFNRAA
                     DWDCWPDESDLERPATNLLVLEPEPTFQAWTQQQPKPKKGTDVPRSLWDVLTFDGDRY
                     RKEGWSTIPGTRLARYVFAAPPFERAVIPSARSMASAERPTVARFAIRSAVLPNIEEA
                     LALGERLRTAAMSHSKRICGDATPVFSGHGQHLSGHRHAMYLAASDDPHNAERGIIDH
                     LIISARMGFEERDIQALQSLRRVWGKTGHDLELILVGLGQPSDYGGHALPKTPLLTES
                     RMWHSLTPFVPTRHPKTVRGIVRDSIEDQLRTACEQLLGLAPISIEPYGERADWAQFR
                     RRRYQGVGHRGPDWALGARLEFAEAVRGPIAIGYGAHFGLGVFVGEE"
     gene            complement(2574..3560)
                     /gene="cas7u"
                     /locus_tag="VFQ61_02555"
     CDS             complement(2574..3560)
                     /gene="cas7u"
                     /locus_tag="VFQ61_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated RAMP protein
                     Csb1/Cas7u"
                     /protein_id="HET9953353.1"
                     /translation="MIMNPFDLPRAPRLLLEAELSPIQGTRFQPTGFPDVGAGVYTLP
                     DGKEQLLVESAQSVANRLELACWDEAKQDLMPELAGLPYVRVLGPEGDLLTTSLQEAH
                     RLNSPYIERTDFFRKDLTAAIDFNDKRPNDRSKLITALARYDIGCLLHGVFLESIAGV
                     LRVPRALSGFIEASDVQRVNTGGVKNDRVQASKDEDTGRTASEGFGNVPFHRTEFVAA
                     KITAYFNLDLEQIRGYRLGADLERLLFALSIFKIRRFLESGLRLRTACDLSARELLVK
                     APSGVELPSLANTLEQLPSLITAARSHFAAPPITSTKFDPGEKKPAKKGSGK"
     gene            complement(3594..>4011)
                     /locus_tag="VFQ61_02560"
     CDS             complement(3594..>4011)
                     /locus_tag="VFQ61_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953354.1"
                     /translation="SDIAAFIDGRVNDALIESWLRAAVLIEGGHRPTVPEDCLLPPAT
                     YAVLAIVHRQRIGQEDLPRTGSLLTLASAGNARAATAAAIRRLNAAARPLPVSELFEP
                     TARTRRIAAALAFPLSERQRRTLERLVLPEPAESQD"
BASE COUNT          713 a         1080 c         1385 g          833 t
ORIGIN      
        1 cttgtggtgc ttggtcttgc cgagtagaat gcgaatcttg tcagattctg gccagttcga
       61 tattgacggc ctttggcggt cttctttggg tccgggctcc cgggccctcc ccaactgcgg
      121 attgtcgaag tccgtggtgc cttgccgaaa gcccctgagc caatcctcaa gcgctacgac
      181 ccaggccgtt gttgcgttgg acggtcgccc cacgtgaaga gccgctcccg caagctgagg
      241 gacggaacgt gcctctggcg acgaatgcga gaatatatcg cgtttgaata ggtgggtgaa
      301 gtgttcccga ctcgcactct gcggcagcca atcttttgca tcttcgagta cgctcagtga
      361 gccgacccca cgacgcgtcc ggctaccgta gccgccaaag acgagccaag cggtgaccgc
      421 gagtctcact tggtcttcga gttgatgtgg gaaaagtgtt ttaagtgtga actggagtcc
      481 cgcgttgaag cgatgagccg tggggatatt cccccgtttc tcttcacgag caggccagag
      541 ggcatagctg ccgggcgcgg tcatacgaat ggggctgtca tctcgcgtac cgggtttcag
      601 aatctgaaca gaaacctcta cctgcgaacg tccaccttcg ttgtctgccg cacggcccca
      661 gatgcgactt tccgccttat agagctcctc cgagcttgcg tattcatggc aatgtagagc
      721 tcgccaccag aaccgaaggt gtccgcggat agtaggagcc cggatggaat caacctcgtc
      781 cagctttcga gtagaagtgc ctcctcccag tatcggcgta atgacctcca ccttgacgtt
      841 caacttggaa cgctcactcg cgccccgtgc tgaacgggaa cgagcttgag gtagagatgt
      901 atacgtcggt gcacagagtg aggactgctt tggtgctggt gaagcaagct ctccactgtt
      961 cggcgatgga atagcagcag tgttccggtt tgagtcgttc atgtctgctt cttgtcggat
     1021 gctctcggca ggattagcga gctgagactg atcttgtttt gatgatgtgt gcgtccgggc
     1081 ccttcactcc tcccccacaa acacccccaa cccaaaatgc gccccatatc caatcgcaat
     1141 gggccctctc accgcctctg caaattcaag ccgtgcccca agtgcccagt ccgggccgcg
     1201 gtgcccaacg ccttgatatc gacgtcgacg aaactgtgcc cagtccgcgc gttcaccata
     1261 gggctcaatg gagatgggcg ccagcccaag cagttgttcg catgcggtac ggagttggtc
     1321 ctcaatgctg tctctgacga tgccacggac ggttttgggg tggcgcgtgg ggacgaaggg
     1381 ggtgaggctg tgccacatgc ggctttcggt gaggaggggg gtcttgggga gggcgtggcc
     1441 gccgtagtcg ctgggttggc ccaggccaac gaggatgagt tcgaggtcgt gaccggtttt
     1501 gccccatacg cggcgtaagc tttgaagggc ttggatgtcg cgctcttcga agcccatgcg
     1561 ggcgctgatg atgaggtggt cgatgatgcc gcgctcggcg ttgtgcgggt cgtcgctcgc
     1621 ggcgaggtac atggcgtggc gatggccgga taagtgctgt ccgtggccag agaagacggg
     1681 ggtcgcatca ccgcagatgc gtttggagtg ggacatggcg gcggtgcgga gccgctcgcc
     1741 aagggccagg gcttcttcga tgttggggag tacggcagat cggatcgcaa agcgtgcaac
     1801 ggtggggcgc tctgcggagg ccatgctgcg tgcgctcggg atcacggctc gctcaaaggg
     1861 aggcgccgcg aagacgtagc gcgcgagtcg ggtgccaggg atggtggacc agccttcttt
     1921 tcgatagcgg tcgccgtcga aggtgaggac gtcccagagg gatcggggga cgtcggtgcc
     1981 cttcttgggt ttgggctgtt gttgcgtcca cgcctggaag gtgggttcgg gttctaggac
     2041 gagtaggttg gtggcggggc gctcgaggtc gctttcgtca ggccaacaat cccaatctgc
     2101 tgcacgattg aaatggtcgt tgggtttctc agaagggcgc acttcaaccc aggattcagc
     2161 gcgaccgagg taggtgatgc gacggcagag ttgctgtaag agggctcgtt cgtcgggtgt
     2221 gagttcaccg ggccaggcga tgatgagggg ttcgggatcg cccgctccgc cttggatggc
     2281 gacgaaggcg tcgaacactt tgctgcgctt gtgttcggca tccgcggtgt caggcatgta
     2341 gtggcgcgtg tgggcttcga cggcgcgggg taggcgatac ttggggaggt cgcggagctt
     2401 gccgagcaag ctagtgaccg cgggttgcgg cgcaccctcg gagtaggctg cgctcacgag
     2461 gctgcgcagg atgcgccaag gggagggcgg ccattcggga acggcttcgt tgtgagcgct
     2521 gtgccagcca gtgccgtgat agcggccggc cagaaaccgc agctcgagtc caatcatttg
     2581 cccgagcctt tcttggcggg cttcttctcc cccggatcga acttggtaga ggtaatgggc
     2641 ggcgcggcaa agtgggagcg cgccgcggtg atgagtgagg gaagttgttc gagggtattg
     2701 gcgagtgagg ggagctcgac tccgctcggg gctttgacca ggagctcccg cgcgctcaga
     2761 tcacaggccg tgcgaaggcg taggcccgat tcgaggaagc ggcgaatttt gaagatggag
     2821 agtgcaaaga gcaggcgttc gagatcggcg ccgaggcggt agccacggat ttgctcgagg
     2881 tcgaggttga agtacgcggt gatcttggcg gctacgaatt cggtgcggtg gaaggggacg
     2941 ttgccaaagc cttcggaagc ggttcgcccg gtgtcttcgt ccttagaggc ttgaactcgg
     3001 tcgttcttga ctccgccggt gttcacccgc tgtacgtcac tggcctcgat gaagccggac
     3061 agcgcgcggg gtacacgcag cacaccggcg atgctctcga ggaatacgcc gtgcaaaaga
     3121 cagccgatgt cgtagcgcgc gagtgcggta atgagtttgc tgcgatcgtt gggccgcttg
     3181 tcgttgaagt cgatggctgc ggtgaggtcc ttgcggaaga agtccgtgcg ctcgatgtag
     3241 ggtgaattga ggcgatgcgc ctcctggaga gaggtcgtta ggaggtctcc ttcggggccg
     3301 agtacacgca cgtacgggag cccggcgagc tctggcatga ggtcttgttt ggcttcatcc
     3361 caacacgcaa gctcgagccg attggcaacg ctctgggcag actcgacgag cagttgctct
     3421 ttgccgtccg gcagggtgta cacgcccgct ccgacatccg ggaagccggt cggttgaaag
     3481 cgagtgcctt gaatggggct gagttccgct tccaggagta gacgaggagc gcgtggcaaa
     3541 tcgaatggat tcataatcat ccctgttcgt gtgaaagctc gatggtaaaa tatttagtct
     3601 tgagattccg cgggttcagg caacaccaac cgttcgagag tgcggcgttg tctctcactg
     3661 agcgggaacg cgagcgcggc ggcgatgcgc cgagtgcgcg cggtgggttc gaatagctcg
     3721 gataccggga gcggtcgcgc ggcggcgttc aatcggcgaa tggcggcggc agtggccgca
     3781 cgagcgtttc ccgcactggc cagcgtgagg agcgagccag tgcgcggcaa gtcctcttgc
     3841 ccaatgcgct gtctgtggac gatcgcgagc acggcatagg tggccggtgg cagcaggcag
     3901 tcttccggta cggtcggtcg atgcccgccc tcgatcaaca ccgccgcgcg tagccaggac
     3961 tcaatcagag catcattgac cctgccgtca atgaacgcag cgatgtcgct c
//
LOCUS       DASTKU010000077         2578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_460033, whole genome shotgun sequence.
ACCESSION   DASTKU010000077 DASTKU010000000
VERSION     DASTKU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460033"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VFQ61_02565"
     CDS             complement(<1..1060)
                     /locus_tag="VFQ61_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9953355.1"
                     /translation="MHPTGNPRVLDKLVEKGLLSSEQREAVLNHHRVVEGRIEEAILE
                     LGFVEETALLKFLAAEHNTRFVSAERLAKADIPMETMAMVPRKLAEHETLFPVLFDAA
                     TGTLSVVTPEPDNVEVLRQLQLGGGPVKVVRALVARPLAIRAAINKFYGGDIYAFAQL
                     DRNAHEQFSNMLNVFERNLVSEESMTAALVREVPAERVLSERDLATGANTRSLFKDGF
                     ASDAYLETLNVLVSLLENNRADLRGHSSQTARLMKKVGERIGLSPSQVAGLQIAGYIH
                     DLGKATAYHLTALNVARYEGHKTAAKRLHRSPVRMLDGVGLPSDAIRAVQYMYERFDG
                     KGLPGELTGKDIPLGARIL"
     gene            1368..1814
                     /gene="rplM"
                     /locus_tag="VFQ61_02570"
     CDS             1368..1814
                     /gene="rplM"
                     /locus_tag="VFQ61_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET9953356.1"
                     /translation="MRTYVAKPEAALADRKWWVLDAKGQPLGRLASLAAHLLRGKHKP
                     SFTPHVDTGDFVIVVNAAEVVVTGDKPNAKKYYRHSGYPGGMTIETFNQMIARRPEVP
                     VEKAVKGMLPKNKLARQMIKKLKVYATAEHPHAAQQPELRKLEGQA"
     gene            1814..2212
                     /gene="rpsI"
                     /locus_tag="VFQ61_02575"
     CDS             1814..2212
                     /gene="rpsI"
                     /locus_tag="VFQ61_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET9953357.1"
                     /translation="MSDTTSQYATGKRKTAVARVWISPNGSGQVTVNGIPADEYFERA
                     TSRMIARQSLAQLELNDKYDINATVAGGGHSAQAEALRHGIARALIAEDPERRLALKQ
                     AGFLTRDARAKERKKYGQPGARKRFQYSKR"
     gene            2319..>2578
                     /locus_tag="VFQ61_02580"
     CDS             2319..>2578
                     /locus_tag="VFQ61_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET9953358.1"
                     /translation="MASPFQLVSEFAPQGDQPRAIQRLMQGLREGEATQTLLGITGSG
                     KTFTVANVIAQWGRPALIIAPNKTLAAQLYGEMKSLFPHNAVR"
BASE COUNT          518 a          836 c          763 g          461 t
ORIGIN      
        1 ccaggatccg ggctccgagc ggtatgtcct tgcccgtgag ctcgccagga aggcctttgc
       61 cgtcgaaacg ctcgtacatg tattgcacgg cgcggatcgc gtcggagggt aaacctacgc
      121 cgtcgagcat gcgcaccgga cttcggtgca gccgcttggc cgcggtcttg tgaccttcgt
      181 accgagcgac gttgagcgcc gtgaggtggt acgcggtcgc tttccccaag tcgtggatgt
      241 atcccgcgat ctgaagacct gcgacctgcg agggcgataa tccgatgcgt tctccgacct
      301 tcttcatgag ccgcgccgtc tggctcgagt gaccgcgcag atcggcgcgg ttgttctcca
      361 gtaggctcac gagcacgttc aacgtctcga gataggcgtc gctcgcgaac ccgtctttga
      421 acagactgcg cgtgttggcg ccggtcgcga gatcccgctc ggacaagacc cgttcggctg
      481 gcacctctcg caccagcgcc gccgtcatcg actcttcgct cacgaggttg cgctcgaaca
      541 cgttgagcat gttcgagaac tgctcgtgcg cgtttctgtc gagctgggcg aatgcgtaga
      601 tgtcaccgcc gtagaatttg ttgatggctg cgcgaatcgc caacgggcgg gccaccaggg
      661 cacggacgac ttttaccggg ccccctccca attgcagctg tcgtagtact tcgacgttgt
      721 ctggctccgg cgtgaccacg ctgagcgtcc cggtcgcggc atcgaacagc accgggaata
      781 aggtctcgtg ctcggccaac ttccgaggca ccatcgccat ggtctccatc ggaatgtcgg
      841 ccttcgccaa acgttccgcg gagacgaagc gcgtgttgtg ttcggccgcg aggaacttga
      901 gcagggccgt ctcctcgacg aaaccgagtt cgaggatcgc ctcctcgatc ctcccctcga
      961 ccactcgatg gtggttgagc acagcctccc gctgctcgct cgagaggaga cccttttcta
     1021 ccaacttgtc gagtacgcga ggattgcccg tcggatgcat tcgagactca aaatttagcc
     1081 caacccgcca cagtagccca tgaccgaacg tcgtttcagt acggcccaac cccacatcgg
     1141 cgcgagcgcc gaatgcacgc agagctggct cgcgcccgga tttggcgcag ctacatcggc
     1201 gcctcagact cctcggccgg ctctgagtgg ggccgcggcc cacaaaaacg caatatcgct
     1261 cagcggcagt gcgccaggtg ccttgacttg cggaacggac accgctaaac gacccgccct
     1321 ccgctcgcag gctgcgagcc atgttcaatt ttcagacagg gtagtcaatg cgcacgtacg
     1381 tcgcaaaacc agaagcagcg ctcgccgacc gtaagtggtg ggttctagac gccaaaggtc
     1441 aacctctcgg ccgcctcgcg agcctggcag cacacctgct ccgcggcaag cacaagccat
     1501 cctttacgcc ccacgtggac accggcgact tcgtgattgt cgtcaatgcc gccgaggtgg
     1561 tggtcacggg tgacaagccg aacgcgaaga aatactatcg ccacagtggc tacccaggtg
     1621 gcatgaccat cgaaaccttc aatcagatga tcgcgcgccg cccggaagta ccggtcgaga
     1681 aggccgtcaa aggaatgttg cccaagaaca agttggcgcg acagatgatc aagaagctta
     1741 aggtgtacgc cacggcggag cacccccatg cagcacagca gcccgagctt cgtaagctcg
     1801 aaggacaagc gtaatgagcg acaccacgag tcagtacgcc accggcaagc gcaagaccgc
     1861 agtggcacga gtttggatca gccccaacgg ttcggggcaa gtcaccgtca acggcatccc
     1921 cgcggatgaa tacttcgagc gtgcgacgtc acggatgatc gctcgtcagt ccttggctca
     1981 gctcgagctc aacgacaagt acgacatcaa cgcaacggtg gctggcggcg ggcacagcgc
     2041 ccaagccgaa gcactccgtc acggcattgc tcgcgctctg atcgcagaag atcccgagcg
     2101 tcgtctggct ctcaagcaag ctggcttctt gactcgcgat gctcgcgcca aggaacgcaa
     2161 gaagtacggt cagcccggcg cacgcaagcg cttccagtac tccaagcgct aagctgccct
     2221 tgggtcggtc cgcgacagcg gatccactgc gaacgaacgg cgccttttgg cgccgttctc
     2281 cattttgtcc gaagccaaag cgcactaccc ttttagccat ggcaagccct ttccaactgg
     2341 tcagtgagtt cgcgccgcag ggggatcagc cccgagccat ccagcggctc atgcaagggt
     2401 tgcgggaagg cgaggccacc cagaccctgc tcggaatcac cggcagcgga aagacgttca
     2461 cggtcgccaa cgtgatcgcg cagtggggtc ggcccgccct gatcattgcg ccaaacaaga
     2521 cactcgcggc gcagctttac ggcgagatga agagcctgtt tccgcacaac gcggtgcg
//
LOCUS       DASTKU010000078        16228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_235968, whole genome shotgun sequence.
ACCESSION   DASTKU010000078 DASTKU010000000
VERSION     DASTKU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16228
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235968"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFQ61_02585"
     CDS             <1..349
                     /locus_tag="VFQ61_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953359.1"
                     /translation="KQGQNGYNTQVENRNWFFAIGGYTTWGKGVAKVTDSGGVLNYEL
                     DFEYKFYDRYNWDAGKSVTFAHITVTDKFMGEFHRQGLAREFDCIGSFRRKFTWKKGE
                     RIPQSQIHPPGGR"
     gene            346..798
                     /locus_tag="VFQ61_02590"
     CDS             346..798
                     /locus_tag="VFQ61_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953360.1"
                     /translation="MSGFFGPIALSLMLAVGALHCSKGTKSMSSTTKPAESLPALSTE
                     LGLSFPASTRLIGVRREAGIDDAVQAKLEMSSADVPAFLASTPLDVSAMRSGTRGFLG
                     ADVDFWDPHKASALKTGQGMLPAGKALNIGVDESRGTIAVVYVMRHGT"
     gene            complement(880..1533)
                     /locus_tag="VFQ61_02595"
     CDS             complement(880..1533)
                     /locus_tag="VFQ61_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953361.1"
                     /translation="MHRTLSTLLVLTSCVAAIACGDDDDMPSRSSGGGGGMGGASSGG
                     TRNGGATSTGTGGDTGGAGENNGAQAGTGQTEFDPQLPPLNAAGAERWLQQKFYEAWA
                     CEEQPNAKTDGAAAIHVHGDKTRVCSNDLLAASSPEAEFPEGVASVKEVYDGDGKLAV
                     TVLSVKGHADSDGGQGWFWYEPPSLAGFGVAACTGCHQAAGSDADHPGAGDFVYFKN"
     gene            1712..4024
                     /locus_tag="VFQ61_02600"
     CDS             1712..4024
                     /locus_tag="VFQ61_02600"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET9953362.1"
                     /translation="MMRESRRSYCATAALFGAIGTAALAACSSTDGRTVMDSEFSQGL
                     TVTDNEPQSAAAYGRNGPGRASSAAELRALIARQVGGLEKLKVPADDVSIPLPPEDPM
                     RPGRYRTTEAKRYLGKLLFHDPVRTVRINVNKNVNPPIRAGEPRDLPEGTAFGGTVSS
                     ANPNVQSVVDATKSTGSCGSCHLGEAAGKAGSVLNFNVGGEGRGYTDEYGNYIIRRRP
                     QKGLIARESAYLPNMKLFEGDTGVDSLPTLTDIYRVGGMLEVATPARQKADPLPEALL
                     ATGRLDQLDSVARQSPSMVGFAFNNRLLLGGFAGESNSLPAGLNPLNDPAQENLTLLL
                     LDAHRMLDLESATLEKIPAFVELFRQAFPEEARDYEACKHANPSAACQPELDELVNDD
                     TVLRATATFLRTAVTRNTPFDRFLAGEDSLTARQRTGAELFFTRAQEGGAGCFTCHSG
                     PMLNKQPNDPDVAGIGKFVEQNFFNVGICDHPVQALNALRRGHLQFDSQGKPLPHGED
                     TGRQEITHNPDDAYKFRSLTLRQLKDARTFFHNGAFASIRDVVSYFNAGVPQDAVFAG
                     HARTLEPRFTNPRGMSAPRGLGLSEEQIDAVTDFLENGLYDSGFVQEFQPNAADLTYS
                     KNHPELAALGAKDGQLLSGLAIDNNDPLSRRDAGLEFLDVSSRARVDRIRNDCSGARV
                     EDVYRITNTSDSVIDTHLLLVVRGLTSKVRLTNASGVTRSGDPYIRVFLPNGVLEPVE
                     RLTQRFVFDNPGNKRMPNYTITLLSGQGTP"
     gene            4176..5141
                     /locus_tag="VFQ61_02605"
     CDS             4176..5141
                     /locus_tag="VFQ61_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953363.1"
                     /translation="MPLEFALRPRFDLERPPPRRRFSGFRLPSFTLPVVAWWLAIGGG
                     ALAWLRAGTADAFEQADAREQPGDAQLLEAQPPAEPPSDGPATLSSAPVADLRAPREP
                     EPLPAAPAQDPEPPVAQVREPEPPPGEADELRSDNQGTSPTRRRTLERELARRPPDRE
                     EPARRPSQSEEPPRALREPAIRSTPIEPPAPNSREAPKEVAREEAPRVEPPAAGLPSC
                     ESAAASANQKIDLRSTRGAPDLTRGAFASVLENGAYFSHCGLGARTALEICAAVQDGK
                     VVGVSVSTEPRSATVNACVRRAVAGLRFPRNAQLDVTRTRFEAER"
     gene            5292..6395
                     /locus_tag="VFQ61_02610"
     CDS             5292..6395
                     /locus_tag="VFQ61_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9953364.1"
                     /translation="MSGTDEGLPRTETDASLRAERQNTDYELARRVTVGDADDDEVLR
                     RARERADRLLAAARAATDARLPLNEQTQAAVELLLEQRGVEDRTLAAERQRADDLLER
                     ERKERREQLSALLVLERQATDLHLGIERQWADKALLARDDFLAQASHDLHGLMAAHRI
                     YLALLVKATGDGEYAQRVTPYASKLLMIDAQIDRIISDLVDTVAIDAGKLAVRLAPTS
                     AAELVAKSTSVFEPLALERGQFISVTPAPADATVMADSARAVQVLGNLLSNAIKFTPS
                     QGEIQVGFDANADVVTFFVSDGGPGVPAEEAEHIFERFVGSNSSSHGLGLGLYISAQL
                     VRAHGGRLWLDRGAQRGATFRFTLNRFNPASGA"
     gene            6546..9074
                     /locus_tag="VFQ61_02615"
     CDS             6546..9074
                     /locus_tag="VFQ61_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9953365.1"
                     /translation="MLSVQRRSGGVWRLPGSRRGGVTTLVVCLGAIPLGAAGCGSDET
                     EAHPAAGGTAAGGGATAATGGTRVTTNQGGAGNAGQVTSPGSGGASEGTAQGGTAGAS
                     GAASGGTAFVGDGGTSGLGGAGSTGTDQGGTGGVIRKCGDAPLGAVESRIRYVVEQAR
                     TAQECAPESQYRSCRETGWSEWSGSNRFPECTVEGCPGTLETRVRFAQPAADYPASCV
                     PEVQFRPCESGVFGAWSGTFEYETCVNRVRDCGAVPHGGSETRTRYQEAEVPFDGKCA
                     SEEQQRVCVNGAFLPWSGSFGSESCRVLQAADCDGKAHGTVQQRVRYAEPKVNNVSEC
                     KSEPQTRTCTDGTWSSWSGSGDASHEACLVRLQGRCTTTRDCQYGVCSSGYCVCQHRS
                     AASCSGEACCGACAPPWTGDNCDQCPASWDKTCSVCSNHWRGRNCDDCPSQFTVESNC
                     TACSPGWSGPNCETQAVCVRFVDPSNTASFQTGYSWREAYANLSTALNDASRYPSCEL
                     WLRAGTYSGTYSPSGEVSLYGGFDGHETSRSERDPEKNPAIFSGGIVPSSTVGAGLII
                     DGITVSGRYIELYNTVLTMRNSKFETGTNVRIEKSKFTLQNSSLFQARNAIFAYAGSQ
                     ISIQNSRFVQSWGPEGAALSLRTGSRARVEDSLFLDGIVGVANSEPGGVMYVSESGLE
                     VSNCVIANNTNRSGSGGAIVVENGEAQIVNATIVNNSVLRPVDSGGAFVVSSDSNVEV
                     VNSILWGNVAPKENVADGPIVIRSSIVEGGFPGDHNMQLAPWFRAPDARDYRLSASSP
                     GIDAADGDSAPERDLNGQVRVDASVADQGTGTPTYADVGAYEWH"
     gene            complement(9102..10634)
                     /locus_tag="VFQ61_02620"
     CDS             complement(9102..10634)
                     /locus_tag="VFQ61_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953366.1"
                     /translation="MSRTRLPLAWSSFGIYLLVAAASVACGKDSDSPLPPANAGRGGT
                     DGGSAAGGTAHVGTNTQSTHAQGGASLGGSGHSGSDSGGASSGGVSGSSAGISGAGSD
                     GSGGAPPDEAGGTAGQTNEPQHPDWPALDCPGPVKVNAAPVESVRLDLQPGTPWLQNV
                     VEPTLSAPTEMGLSASIALLFGDDYTSARLDFPAQVLDPRCCCTLLGSGLTLTNVSDV
                     EGAVDATLEGNASVRVTARSLGTGSFTVRGTMTFPAGQLASCAAYEQASALPIFWKVS
                     AVVREPKWRLQNMPGCAANAAMLSGQALPIWVAAYDGETLISPANAAHPAIEITGEGV
                     SLRVPESARPQEVIAEGDGKVRLSIPGTDLVHELRVVPPSKLDKLEIAWALAGRGGAM
                     RQLSTGETYTCGARTGNRLVAVVSKAYTDDLELCGPILTQDINLRSLTPETCQVDAQL
                     CEIVEIPDGDLIQRTAEIKTDGVCAVRLEAPEYARGQGLEETLSVTLSNTDNLGDLTP
                     GD"
     gene            complement(10792..11382)
                     /locus_tag="VFQ61_02625"
     CDS             complement(10792..11382)
                     /locus_tag="VFQ61_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9953367.1"
                     /translation="MPYAADHKLKTRARIVDSARVLFNRHGFDAVSIDDVMKNAGLTR
                     GGFYHHFTSKASLYAAAVSSFTSCNPFAERLEQNPKRRSARELAALLVDTYLSDETLA
                     DVDKHCPLIALPSDVARAGLQPRAAYTQLVESMLEVFRAAFPKGDRQGNRKAQLIVNL
                     CVGGMLIARTTDEPTLRKQLRSAARSEALRLLATSE"
     gene            11464..12153
                     /locus_tag="VFQ61_02630"
     CDS             11464..12153
                     /locus_tag="VFQ61_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9953368.1"
                     /translation="MTTSTTISPQAVRVSVLAWAPPMVHGLVRDLRVRWALEEAGWPY
                     HESVVNRAEQASAKYRELQPFGQIPAMECGDLKLFESGAIVHYIAERSPALMPEDAAA
                     RSQVTAWMFGALNTVEPPVMMLSILDMIYQGPKGDDYRGIRAWVAGWVESRLDVLAGV
                     LEGKEYVLGRFSAADVLLTTVLRVLRDTDFVKKRAVLEVYQRRCEARPAFQKALKDHL
                     ENFARHAPTNV"
     gene            complement(12150..12905)
                     /locus_tag="VFQ61_02635"
     CDS             complement(12150..12905)
                     /locus_tag="VFQ61_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953369.1"
                     /translation="MTSYERCWAKAVSNCGEGSDESLSKPDRGETRVDISLPQGLCGK
                     HETELSPLDNALNDLMNCAREVERLRVLRAAIARKDWPQVHLTADGARIERCVLKMLM
                     THVTVQPESVGDWHPPEWLPLVIFGQRALEAGCGLGVVARVGDVVTNSEEVNFVLGQP
                     ESTGQRHSALLEIRGGWRLICSWDRPLAALGNLRFTGTTPAAGEDVLHHPRQIKFRHG
                     QRDLGVSLDFDWSGKWTPSKHRSVVALRAKRLP"
     gene            13091..13876
                     /locus_tag="VFQ61_02640"
     CDS             13091..13876
                     /locus_tag="VFQ61_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HET9953370.1"
                     /translation="MFDWWRRLNRAEVIAEPFPQPFHELILRRVPCAKLLQPEELAKL
                     EALVRIFNSEKTFEAAGGLTLTEEMRIVIAARACLLLLHRIELDSPLYPELQTVIVYP
                     STYRVRAERREGYVTIESDEVRLGESWTRGIVVLSWDAIESGSANPTDGHDVVLHEFA
                     HQLDSEDGAMDGTPELDNLERYSVWSRVAGAEYAALLDDLEHHRKTSIDAYGATNAAE
                     FFAVVVEQFFEKPQSLERRHPSLYAELSRFFRFDPAQRLRERL"
     gene            complement(13902..14462)
                     /locus_tag="VFQ61_02645"
     CDS             complement(13902..14462)
                     /locus_tag="VFQ61_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953371.1"
                     /translation="MAHSDVRKVLGLGLGLLFGSILATSCTVALDAASLESGCPANTK
                     QCDGSCVPADDPELGCGRTTCQPCALPKASSVCGPDGECLVASCSGSYENCNDDSRDG
                     CEVNTDSDPQHCGSCNAAPCTLANALPDCSGGQCAILRCITGFKDCDGRADNGCEVVL
                     STDKDNCGDCNRRCATGQTCSSGTCG"
     gene            complement(14449..15354)
                     /locus_tag="VFQ61_02650"
     CDS             complement(14449..15354)
                     /locus_tag="VFQ61_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953372.1"
                     /translation="MKHRHSPPRLNHSLLAAALCSLVFGYSAQAAAAPPSRDACVQSH
                     GKSQELRLDGKLLETRTELRICAESSCPEMVRSDCTAWLVETERQIPSFVVVIETDQG
                     DLTTARAFVDGERLPSTNDDTAYERNPGIHRLRFELDGYPAIEDTVILRQDEKRSVVR
                     ARFQREAPQAAEASGAASAVKPGAPAITGPRPIPLTTYVFGGAALAAGGLAGYFGFKA
                     TSDYSDRKDSCAPNCPDSDVSTIKRNALIADVAGGAALISAGLATYFYVTRPARPDHS
                     ALGQARNFDILINSNRIHLGLHGAF"
     gene            complement(15405..>16228)
                     /locus_tag="VFQ61_02655"
     CDS             complement(15405..>16228)
                     /locus_tag="VFQ61_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9953373.1"
                     /translation="CGALSDSLLESELFGHQRGAFTGAGEAKPGMLELGDKGTVFLDE
                     IGELPLHTQVKLLHALETGEVTRVGATRSRAIDVRYVAATNRDLPVEVSEGRFRRDLF
                     FRINAISLDLPPLRQRKSEIEPLARHFLKRFCYSGGIAEPTLSREAVEHLVNHSWPGN
                     VRELRNAVERAVILCRGSEILPRDLPDGKRIAASLRCRPSGIDPWESDTETTVPVWDI
                     GAGLAHLSDSSRVMEAIRLCQGNQTRAAQLLGISRRTLVNRLNEYQLPRPRKRPV"
BASE COUNT         3032 a         4954 c         5246 g         2996 t
ORIGIN      
        1 gaagcagggc caaaacgggt acaacacgca ggtggagaat cggaattggt tcttcgcgat
       61 cggcggttac accacgtggg gtaagggcgt ggcgaaggtc acggattctg gcggcgtact
      121 gaattacgag ctcgacttcg aatacaagtt ctatgaccgc tacaattggg acgccggcaa
      181 gtcagtgacg ttcgcgcaca ttacggtgac cgacaagttc atgggggagt tccatcgcca
      241 gggtctggcg cgcgagttcg actgcatcgg gagcttccga cgcaagttca cctggaagaa
      301 gggcgaacgc attccgcagt ctcagatcca cccccccgga ggccgatgag cggttttttc
      361 gggccgatcg ccttgagtct gatgttggcc gtaggtgcac tccactgcag taagggaacc
      421 aagagtatga gctcgacgac gaaaccagcc gagtcgctgc ccgccctgag caccgagctc
      481 gggctttcat ttccggccag tacgcggctg atcggggtca ggagagaggc cggaatcgac
      541 gacgcggtgc aggccaagct ggagatgagc agcgcggatg tgcccgcatt tctcgcgtcg
      601 actccgctag acgtcagcgc catgcgctcg gggacccgcg ggtttctcgg tgccgacgtg
      661 gacttttggg atccccacaa ggcctcggct ctgaagacgg gccagggcat gctgcccgcg
      721 ggcaaggcgc tgaacattgg agtcgacgag agccgcggca ccatcgccgt ggtttacgtg
      781 atgcgacacg ggacttagcg gtgctctctc ctctcggccc gcttgctgcg actgacctct
      841 tcattcgcgg acccttagtc ccactcgagc gggcgctcac tagttcttga agtagacgaa
      901 gtcgccggcg cccgggtggt cggcgtcgga acctgcggct tggtggcaac ccgtgcaggc
      961 agcgacgccg aagcctgcca agctgggcgg ctcgtaccaa aaccatcctt ggccgccatc
     1021 gctgtcggcg tgccctttca cgctgagcac ggtcacggcg agtttgccgt cgccgtcgta
     1081 cacttctttg acggaggcga ctccctccgg gaattcagct tctggggatg acgctgcgag
     1141 taggtcgttg ctgcagacgc gcgtcttgtc tccgtgcacg tggattgcgg cagcgccgtc
     1201 cgtcttcgcg ttcggctgct cctcgcaggc ccaagcttca taaaacttct gttgcaacca
     1261 gcgctcggcc ccggccgcgt tcaggggcgg gagctgcgga tcgaattcgg tctgaccagt
     1321 gccggcctgg gctccgttgt tttctcccgc gccgccagtg tcgccgccgg ttccagtgct
     1381 tgttgcgccg ccgtttcgag tgcctccgga gcttgctcca cccattcccc cgcctccgcc
     1441 gctgctgcgg gagggcatgt cgtcatcgtc accgcatgcg atggctgcaa cacatgaagt
     1501 gagaacgagc agggtgctca atgttcgatg catgggcatt gggaaagctc ctgttcttgg
     1561 agaaagaaga cgacttccat ccgtacgtcg cacccgggtg acggttacca acaaactctt
     1621 cggcgcggta accaccgggt cgccctcccg tagtggggca atgcgcgcgc tgggcgcgac
     1681 gccaagaaag cgaatcggga ggaacagact catgatgcga gaatcacgcc gcagctactg
     1741 cgcgacggcc gcgctgttcg gagccattgg cacggccgcg ctagccgcct gctcgagcac
     1801 ggatggacgg acggttatgg acagtgagtt cagccaaggg ctgacggtca cggataacga
     1861 gccgcagtcc gccgccgcgt atggtcgaaa cgggccaggt cgcgcttcga gcgcggccga
     1921 gcttcgagcg ctgattgctc ggcaggtggg tggactcgag aagctgaagg ttccagcgga
     1981 tgacgtttcc atcccgctgc cgccggaaga cccaatgcgt cccggacgct atcgcacgac
     2041 ggaagccaag cgttaccttg gaaagctact gtttcatgac ccggtgcgaa cggtgcggat
     2101 caacgtcaat aagaacgtaa atccacctat ccgagctggc gagccgcgag acttgcctga
     2161 aggcaccgca ttcgggggaa ctgtctcgag cgccaacccg aacgtgcaga gtgtggtgga
     2221 cgcgaccaag agcaccggat cgtgtgggag ctgccacttg ggcgaggcgg cgggcaaggc
     2281 tggttcggtg ttgaacttca atgtcggtgg cgaaggccgc gggtacacgg acgaatacgg
     2341 caactacatc attcgccgcc gccctcaaaa ggggctgatt gcgcgtgaga gtgcctatct
     2401 gcccaatatg aagctgttcg agggtgacac gggggtcgac tcattgccca ccctgaccga
     2461 tatttatcgc gtgggaggga tgctggaagt cgcaactcca gcacggcaga aggccgaccc
     2521 gctgccggaa gcgttgctcg cgaccgggcg cctcgatcag ctagatagtg tggcacgtca
     2581 gtctccgtcg atggttgggt ttgcgttcaa caaccgtctt ttgcttggcg gtttcgcggg
     2641 agagtccaac tcattgcccg ctggactgaa tcctttgaat gatccggcgc aggagaacct
     2701 gactctgttg ttgctcgatg cccaccggat gctggatctc gagtccgcga cgttggagaa
     2761 gatccctgcg ttcgtggagc tgttcaggca agccttcccc gaagaggctc gcgactacga
     2821 agcttgcaag catgccaatc cgagcgcggc gtgccagcca gaactggacg agttggtcaa
     2881 tgacgacacg gtgttgcgcg ctacggcaac attcctgcgc accgccgtca ctcgtaatac
     2941 ccccttcgac cggttccttg ccggagaaga ctcgctgacc gcgcgccagc gcaccggcgc
     3001 cgagctgttt ttcaccaggg cgcaggaagg cggcgccggg tgcttcactt gtcacagcgg
     3061 tcccatgctc aacaagcaac cgaacgaccc agatgtggcc gggattggaa agtttgtaga
     3121 acagaacttc ttcaatgtcg gaatttgcga ccaccccgtt caggcgctga atgccctacg
     3181 ccgagggcat ctccaattcg attcgcaagg taagcccctc ccccacggag aagacacggg
     3241 tcgtcaagaa attactcaca atcccgacga cgcgtacaag ttccgcagcc tcaccctgcg
     3301 acaattgaaa gacgcgcgta cgttcttcca caatggtgca ttcgccagca tccgcgacgt
     3361 cgtgagctac ttcaacgccg gggtccctca agatgccgtg tttgccggtc acgcgagaac
     3421 gctggagcct cgctttacca atccgcgggg catgagcgcg ccgcgcggcc tgggcttgag
     3481 cgaagaacag atcgacgcgg tcaccgactt cttggagaac ggcttgtacg attcgggatt
     3541 cgtgcaggag ttccagccga acgcggccga tctgacctat tccaagaacc accccgaact
     3601 ggccgctctc ggcgcgaagg atgggcagct cctcagcggt ctcgcgattg acaacaatga
     3661 ccctctctct cggcgcgatg cggggctcga gtttttggac gtcagctcgc gagcgcgcgt
     3721 cgatcgaatc cgaaacgact gttcgggggc cagggtggag gacgtttacc ggatcaccaa
     3781 cactagcgac tcggtcatcg atactcactt gttgttggtg gtgcgcggac tgaccagtaa
     3841 agtccggctc accaatgcga gtggcgttac gcgaagcgga gacccttata tccgcgtctt
     3901 cttgccgaac ggcgtgctcg agccggtcga acgactcact cagcgcttcg tcttcgacaa
     3961 ccccggcaac aaacgcatgc cgaattacac gatcactctg ctatccgggc agggcacgcc
     4021 ctgagcgccc caaaacgagc cgatagcccc gaggattggc tctaagaaaa cttcagagca
     4081 gtaggatctg ctgcgcaagc gttcaagttc acccggcccg cggtgcacag cgggcttggt
     4141 gaagtgggca ggacggtgca aactggcgcg ccccgatgcc cctcgagttt gccctccggc
     4201 cgcgcttcga cttggagcga cccccgcccc gcaggcgatt ctcgggattc cggctgccat
     4261 ccttcacatt gcctgtggtc gcgtggtggc tggcgattgg gggaggcgcg ctggcttggc
     4321 tcagggccgg tactgccgat gcgttcgagc aggcagacgc tcgagaacaa cctggagacg
     4381 cgcaattgct cgaagctcag ccgccagccg agccaccgag cgacggccct gcaacactct
     4441 ccagcgctcc cgtcgcggat cttcgggcac cgcgcgagcc ggagcctttg cccgcagctc
     4501 cagcacagga ccccgagccg cccgttgccc aagtgaggga gcctgagcca cctcccggtg
     4561 aggccgacga acttcgctcg gataaccagg gcacgagccc gacgcgccga cgtaccctgg
     4621 agcgcgagct cgcgcgccgc cctccggatc gtgaagaacc ggcccggcgc ccaagccaga
     4681 gtgaggagcc gcccagagct ctacgcgagc ccgcaattcg gtccacgcca atagaaccgc
     4741 cggctccgaa ctcgcgcgag gctcccaagg aagtggcccg tgaggaggcg ccacgagtcg
     4801 agcctcccgc tgcgggactg ccgagttgcg agagcgcggc cgcgagcgca aaccagaaga
     4861 tcgatcttcg ttctacccgc ggcgcgccgg atctcacccg tggcgcgttc gcctccgtgc
     4921 tcgagaatgg cgcctacttc agccactgcg ggctcggtgc gcgcacggca ctcgagatct
     4981 gcgcggccgt acaagacggc aaagtcgttg gcgtcagcgt gagcaccgaa ccccggagcg
     5041 ccaccgtcaa cgcttgtgtt cgacgcgcgg ttgccggact tagatttccg cgcaatgcgc
     5101 agttggacgt tacccgaacg cgcttcgaag cggagcgctg actggggggg gtgaaggagc
     5161 ggggtgaagg agcgaacgtg ggagaagcta gctacgctaa gccgtccggc gctgagagct
     5221 cctgaccgac gcgcgtccga gagagcaaag tttcctgcaa acgtcggggg aagactataa
     5281 ttgctcctac gatgagtggc actgatgagg gcctaccccg aaccgagacc gacgcgagtc
     5341 tgcgggcgga gcgtcagaac acggactacg agctcgcgcg acgcgtgacg gtcggagacg
     5401 cagacgatga cgaggtcctt cggcgagctc gtgaacgcgc ggaccggttg ctcgcggcag
     5461 cacgggcggc cacggacgcc cgcttacctt tgaacgagca gacgcaggcg gcggtggagc
     5521 tgcttctcga gcagcgcggc gtggaagatc ggactcttgc ggccgagcga cagcgagccg
     5581 atgatctgct cgagcgcgag cgaaaggagc gtcgagagca actttcggcg ttactcgtgc
     5641 tcgagcggca agccaccgat cttcatctcg gcatcgagcg acaatgggca gacaaagccc
     5701 tgctcgcgcg cgacgatttt ctggcccagg ccagccacga tcttcatggg ctcatggcgg
     5761 cacaccgcat ctacctggcg ctgctcgtca aggctacggg cgacggagaa tatgctcagc
     5821 gggtcacgcc ctatgcctcg aagttgctga tgattgacgc gcagatcgat cgcattatca
     5881 gcgatctggt cgacaccgtc gcgatcgatg ccggcaaact cgccgtgagg ctcgctccca
     5941 cctccgcagc cgagttagtt gccaagtcaa cctccgtgtt cgagccgctc gccctcgaac
     6001 gcggtcaatt catatcggta acacccgccc ccgcggacgc gaccgtcatg gccgatagcg
     6061 cgcgtgcggt tcaggttctg ggaaacctac tttcgaacgc catcaagttc actccgtcgc
     6121 aaggcgaaat tcaggtcggt ttcgatgcga acgccgatgt agtcaccttc ttcgtctccg
     6181 acggcggccc tggagtaccc gccgaggaag ctgagcacat cttcgagcgc ttcgttggct
     6241 cgaacagctc ctcgcatggc ctcggcctcg gcctgtacat ctcggctcag ctcgttcggg
     6301 cccatggcgg gcgtctttgg ctcgaccgcg gcgcgcaacg cggcgccacc ttccggttca
     6361 ccttgaatcg cttcaatccg gcttccggag cctgagcgct gacgctcggg atcactaaac
     6421 gcaggccgat agatcccagc cagcaaaccc caaagaattg tgtggcgatc cgtcaggact
     6481 tccggggata ccgctgacgc gagccaggat tgggagtatg agggagcgtt atttggagtg
     6541 tgaaaatgct atcggtgcaa cgtcggtcgg gtggagtttg gcggcttccg gggagccggc
     6601 gcggcggcgt taccacgctg gtagtctgtc ttggcgcgat tcccctcggc gctgccggtt
     6661 gcggcagtga cgagacggaa gctcaccccg cagcgggagg gaccgccgcc gggggcgggg
     6721 ctaccgccgc gacaggcggt acccgggtta cgaccaacca gggcggggcg ggtaatgcag
     6781 ggcaggtgac ttcacctgga agtggagggg cttccgaggg cacggcccag ggcggaactg
     6841 caggcgcgag cggtgccgcg tcgggtggga ccgcattcgt tggtgacgga ggtacttcgg
     6901 gcctcggcgg cgcaggatcg acgggtactg accagggcgg cacgggggga gtcattcgta
     6961 agtgcggaga cgcgccactt ggcgcggtgg agtcgcggat ccgctacgtg gtggagcaag
     7021 ctcgaacggc acaggagtgc gcgcccgaat cgcagtatcg aagctgtagg gagacgggtt
     7081 ggtccgaatg gtcgggctcc aatcggttcc ctgaatgcac ggtcgagggc tgccccggta
     7141 cactcgaaac ccgcgtgagg tttgcccagc cggccgcaga ttatcccgct tcctgcgtgc
     7201 ccgaggtgca gtttcgtccg tgtgaaagcg gggtcttcgg cgcttggtcg gggaccttcg
     7261 agtatgaaac gtgtgtcaat cgagttcgcg attgcggcgc cgtcccccat ggcggaagcg
     7321 agactagaac tcgctaccaa gaggctgagg tgccgttcga cgggaagtgt gcctccgagg
     7381 agcagcagcg ggtctgcgtg aacggcgcgt ttttgccgtg gtcgggcagc tttggttcgg
     7441 agagttgccg cgtgctgcaa gccgccgact gcgatggcaa ggctcacgga accgttcaac
     7501 aacgtgtgcg ttacgccgag ccgaaggtga acaacgtgtc cgagtgcaag agtgagccgc
     7561 aaacgcggac ctgcacggat ggcacttggt cgagctggag cggctcgggg gacgcgagcc
     7621 acgaagcctg cctcgtgcgt ctgcagggcc gctgcacgac gacgcgagat tgccaatatg
     7681 gagtttgcag ttcgggttat tgcgtgtgcc agcaccggag tgccgcgtcg tgctcgggcg
     7741 aggcctgctg tggcgcctgc gctccgccgt ggacggggga caattgtgac caatgccccg
     7801 ccagctggga caagacgtgt tcggtgtgca gcaatcactg gcgcgggcgg aattgcgacg
     7861 actgtccttc tcagttcacg gtcgaaagca actgcacggc ttgctcaccg ggctggtcgg
     7921 ggccgaactg tgagactcaa gcggtctgcg ttcgcttcgt ggatccgtcc aacaccgcga
     7981 gtttccaaac tgggtattcc tggcgcgagg cgtacgccaa tctcagtacc gctttgaatg
     8041 acgcgagccg ctacccgagt tgtgaactct ggctccgcgc gggtacctac tccggaacct
     8101 atagtccgag cggtgaggtg tcactttacg gcgggttcga tggtcacgag acgagccgtt
     8161 cggaacgtga cccggagaag aatcccgcga tcttcagcgg cgggatcgtc cctagtagca
     8221 ccgtgggcgc aggactcatc atcgacggga tcacggtcag tgggcgctat atcgaattgt
     8281 acaatacggt gctgacgatg cgtaacagca aattcgagac gggcacgaac gttcgaatcg
     8341 agaagagcaa attcacgctc cagaactcct cgctcttcca ggcccgcaac gccatcttcg
     8401 cctatgccgg gagtcagatt tcgatccaga acagccgctt cgtgcaaagt tggggccccg
     8461 aaggtgccgc gctgagctta cggaccggat cgcgtgcacg agtggaagac tcgctgtttt
     8521 tggacggcat cgtcggcgtc gcgaattcgg agccgggcgg cgtcatgtac gtctcggaaa
     8581 gtgggctcga agtgagcaac tgtgtgattg ccaacaatac caatcggagc ggctcggggg
     8641 gcgcgatcgt ggtcgaaaac ggggaagccc agatcgtcaa cgccacgatc gtgaacaaca
     8701 gtgttttgcg tcccgtggac tcaggtggtg cgttcgtggt atcgagcgac tcgaacgtgg
     8761 aagtcgtcaa cagtattctt tgggggaacg tagcacccaa ggaaaacgtg gcggacggcc
     8821 cgatcgtgat ccgcagcagc atcgtcgagg gcggctttcc tggcgatcac aacatgcaac
     8881 tggcgccgtg gttcagagct cctgatgccc gtgactaccg cctcagcgcc tcttcacccg
     8941 gtattgacgc cgcggatggc gatagcgccc ccgagcgaga cctgaacggg caagttcgcg
     9001 tcgatgcatc ggttgccgat caaggcaccg gaaccccgac gtacgcagac gtcggagctt
     9061 acgagtggca ttgagcgccg cgaccgcgac cgcagttcga ctcaatcccc cggcgttagg
     9121 tccccaagat tgtccgtgtt cgagagggtg acactcaacg tctcttcgag gccttggccg
     9181 cgggcgtatt caggggcttc taagcgcact gcgcagacgc cgtcggtctt gatctcggcg
     9241 gtgcgctgga tcaaatcgcc gtccgggatt tcgactatct cgcagagttg cgcgtctacc
     9301 tggcaagttt cgggggtgag ggagcgcaag ttgatgtctt gggtgaggat gggtccgcac
     9361 aattcgagat cgtccgtgta agccttcgat acgacggcaa ccaggcgatt gccggttcgg
     9421 gcgccgcagg tgtaggtctc accagtgctc agctgtcgca ttgcgccccc gcgccccgcc
     9481 aaggcccagg caatttccag tttgtcgagc ttgctcgggg ggacgacgcg gagctcgtgc
     9541 acgaggtcgg tgccgggtat ggagagccgc actttgccat caccttctgc aatcacttcc
     9601 tgcggtcgcg cgctttctgg aacccgaagg ctcacgcctt ctcccgtgat ctcgatcgct
     9661 ggatgtgcgg cattagccgg agaaatcaga gtttcaccat cgtatgcggc cacccagatg
     9721 ggtagtgctt ggccggaaag catggccgcg ttcgcggcac accctggcat gttctggagc
     9781 cgccacttcg gctctcgaac gaccgcactc actttccaga atatgggcag ggctgacgcc
     9841 tgctcgtagg ctgcgcaact tgcgagttgc cctgccggga aagtcatggt cccgcgcacg
     9901 gtgaatgaac ccgtgccgag tgatcgagcg gtgactcgta cgctggcgtt cccttccaaa
     9961 gtagcgtcta ccgcaccctc gacgtcgctc acgttcgtga gcgtcagccc gcttccgagc
    10021 agtgtgcagc agcagcgtgg gtcgagcacc tgggctggga aatcgagccg ggcggaggtg
    10081 tagtcatcgc caaagagtaa ggcaatgctg gcgctcagcc ccatctccgt cggggccgag
    10141 agggtcggtt cgaccacgtt ttggagccag ggagtgccgg gttggaggtc caaccgaacg
    10201 ctctcgaccg gggccgcgtt caccttcacc ggaccgggac aatccagggc gggccaatcg
    10261 gggtgttgcg gttcattcgt ctgccccgcg gttcctcccg cttcgtcggg tggggcccct
    10321 ccagagccat cgctacccgc gccgctgatt cccgcgctgc tgccgctcac accaccggag
    10381 ctcgcgcctc cggagtcact accgctgtga ccagagcccc ctaggctggc gccgccctgt
    10441 gcgtgcgtag actgggtgtt cgtgccgacg tgcgcagtac caccagccgc actcccgcca
    10501 tccgtaccac cacgcccagc gttcgccggt ggaagcggag agtccgagtc tttaccgcag
    10561 gcgaccgatg cggccgcgac aagcaggtaa ataccaaatg acgaccaagc aaggggcagc
    10621 cgagtacgcg acacgcccgc attctacccg aaaccaagag cccaacccag tggaagctat
    10681 tcgccggcag cagatagccg cgtccaaatt gcaggcgggc cgttcgagcg gcgaagcgtg
    10741 gaattcgtcg agtgatacgc ggtctagcac cgcagtgact tcctagtctg ctcattcgct
    10801 ggtcgccaag agtctcaggg cttcactgcg ggccgcggac cgcagttgct ttcgcaaggt
    10861 cggttcgtcg gtggtgcggg cgatgagcat gccgcctaca cacaggttga cgatgagctg
    10921 cgctttgcga ttgccctgac gatcgccctt cgggaacgca gcgcgaaaca cctcgagcat
    10981 gctctctacc aattgagtgt aagctgctct cggctgcaag ccggcgcgcg caacgtccga
    11041 cggcagagca atcaacgggc aatgcttgtc gacatcggcc agcgtttcgt cgctcaagta
    11101 cgtgtccacc aagagcgcgg cgagctctct cgctgagcgt cgcttggggt tctgctccag
    11161 ccgttcagcg aacggattgc agctggtgaa gctgctaacg gcggctgcgt acagcgatgc
    11221 cttgctggtg aagtgatggt agaagccgcc gcgagtgagg cccgcattct tcatcacgtc
    11281 atcgatgctg acggcgtcga agccgtgacg attgaagaga acgcgcgcgc tatcgacgat
    11341 gcgcgcgcgg gttttgagct tgtgatctgc ggcgtaaggc atccgggctc cgctgggttc
    11401 gaacagaata tgttattgaa catattctgt ccgagggcaa cctgcctgac acaggagaca
    11461 gccatgacga ccagtacgac aatctcgccc caagccgtcc gagtcagcgt tttggcttgg
    11521 gcgccgccga tggtacacgg gttggttcgc gatctacgcg tgcgctgggc gctcgaggaa
    11581 gcaggctggc cctaccacga aagcgtggtc aatcgggcgg aacaagcgag cgctaagtac
    11641 cgtgaactgc agcctttcgg gcaaattcct gcgatggagt gcggggattt gaagctgttc
    11701 gagtcaggcg cgatcgtgca ttacatcgcg gagcgcagcc ccgcgctcat gcccgaagat
    11761 gccgcggcta ggtctcaggt cacggcttgg atgttcggag cactcaacac ggtggaaccc
    11821 ccggtcatga tgctcagtat cctcgatatg atctatcagg ggccgaaggg cgatgattac
    11881 cgggggatac gcgcttgggt agcgggttgg gtcgagtcgc gcttggacgt gctcgcggga
    11941 gtgctggaag gcaaggagta cgtgctcggc cgcttcagcg ccgcagacgt gttgctgacg
    12001 accgtgctac gggtgctacg cgacacggat ttcgtgaaaa agcgagccgt gctcgaggtc
    12061 tatcaacgcc gctgcgaggc ccgaccggcg tttcagaaag cattgaagga ccacttggaa
    12121 aactttgcca gacacgcgcc gacgaatgtt tagggtaaac gcttggcgcg caacgcgacg
    12181 acgctgcgat gcttgcttgg agtccatttg cctgaccagt cgaagtcgag cgatacgccg
    12241 aggtctcgtt ggccatgcct gaacttgatt tgccgcgggt ggtggagcac gtcctcgccc
    12301 gcggcagggg tggtcccggt gaagcggagg tttccgagcg cagcgagcgg tcgatcccag
    12361 gagcagatca ggcgccatcc gccgcggatt tccagcaaag cgctgtgacg ttgacccgtg
    12421 ctctccggct ggcccaacac gaagttgacc tcttccgagt tggtcaccac gtctcctacc
    12481 cgggccacga caccgagccc gcagcccgct tcgagcgctc gctgaccgaa gatcacgagc
    12541 ggtagccact ccggtggatg ccaatcaccg actgactccg gttgaacggt cacatgggtc
    12601 atcagcatct tcaaaacgca gcgctcgatg cgggctccgt cagccgtgag atgcacctgg
    12661 ggccaatctt tgcgggcgat cgcggcccgc agcacccgca gccgctcgac ctcccgagca
    12721 cagttcatca agtcgttcag cgcgttgtcg aggggcgaca gttcggtttc gtgtttccca
    12781 cacaagccct ggggaaggga aatatcgacg cgagtctccc cgcgatcggg tttcgagagg
    12841 ctttcgtccg atccttcacc gcaattcgag accgctttgg cccagcaccg ttcgtaagac
    12901 gtcacagcgg ggccaaggct ggcacagatc gggcacaaag cgacgataat cggctaaccg
    12961 cccgcgcgct gctcagggtt gcggctcagg gttgctctac cgatagcggt gctctgctaa
    13021 cgggcaacac ggccgcgagg gcgaggcttt ctcggtggtt tcgtcgggct tcgtgctcta
    13081 gggtgggggc atgttcgatt ggtggaggcg tctgaatcgt gccgaagtca tcgccgagcc
    13141 tttcccccag ccgtttcacg agctgatcct gcggcgggtg ccatgcgcca agcttctgca
    13201 gcccgaagag ttggccaagc tcgaggccct ggtgcggatt ttcaactcgg agaagacgtt
    13261 cgaggccgcg ggcggcctca cgctgaccga ggagatgcgc atcgtaatcg cggctcgcgc
    13321 ttgcctgctg ttacttcacc gcatcgagct cgacagcccg ctttatccag aactacagac
    13381 cgtcatcgtc tatccctcca cctatcgggt tcgggcggag cgacgcgaag gttatgtgac
    13441 gattgaaagc gacgaagtgc gtttaggcga gtcctggacg cggggcatcg tagtactctc
    13501 gtgggacgcg atcgagtcgg gctccgcaaa tccaacggat ggccacgacg tcgtcctgca
    13561 cgagtttgct catcagctag acagcgaaga tggggcaatg gacggcacac ccgagctcga
    13621 caatctcgag cgctattcgg tttggtccag agtcgcgggc gcggaatatg cggccctgct
    13681 ggatgatctc gaacaccatc gcaaaaccag catcgatgcc tacggtgcta ccaatgccgc
    13741 cgagttcttc gcggtcgtgg tggagcagtt cttcgaaaag ccgcagagcc tcgagcggcg
    13801 acacccgagc ctctacgccg agctcagccg attcttcagg ttcgacccgg cccaacggct
    13861 gcgagaaagg ttgtagtcgt gtcgtttgga gtcggctcgc gctagccgca ggttccactc
    13921 gagcaggtct ggccggtggc gcagcgacgg ttgcaatcgc cgcaattgtc cttatcggtg
    13981 ctcaacacca cttcacagcc attgtccgca cgaccatcgc aatccttgaa gccggtgata
    14041 cagcgcagaa tggcgcactg gccaccggaa caatcgggca gggcgttggc cagcgtgcag
    14101 ggggccgcgt tgcaagagcc gcagtgttgc ggatccgaat ccgtgtttac ctcgcagcca
    14161 tcgcgagagt cgtcattgca gttctcgtag gaaccactgc aggaagcaac caaacactcc
    14221 ccgtctgggc cacagaccga ggacgctttg ggcaacgcgc agggctggca ggtggtgcgt
    14281 ccacagccga gctcgggatc gtcggcagga acacagctgc cgtcgcattg cttggtgttt
    14341 gccgggcagc ccgactccaa ggaggcggcg tcgagcgcca cggtgcagga ggttgccagg
    14401 atcgagccga acagcagccc caagcccaag cccagtactt tacgaacgtc agaatgcgcc
    14461 atgcaggccc aagtgaattc ggttgctgtt gatcagaata tcgaagtttc gtgcttggcc
    14521 cagcgcggag tgatcgggac gggcgggccg agtcacgtaa aagtaggttg ccaatccggc
    14581 gctgatcagc gcggccccgc ctgcaacatc ggcgatcaag gcgttacgct tgatggtact
    14641 cacgtcggag tcaggacaat tcggtgcgca gctgtcctta cgatccgaat agtcgctagt
    14701 ggccttgaat ccgaagtagc cggcgaggcc acccgcggcg agtgcagcgc ctccgaagac
    14761 gtaagtggtc aaaggaatcg gacgcggtcc cgtgatggcg ggggcacccg gtttcaccgc
    14821 cgatgcggcc cccgaagcct cagccgcctg cggcgcctct ctctgaaacc gagctctcac
    14881 cacacttcgc ttttcatcct gtcgcagaat gacggtgtcc tcgatggcag gataaccgtc
    14941 gagctcgaag cgtaagcgat ggatccccgg gtttcgctcg taggcggtat cgtcgttcgt
    15001 ggacgggaga cgctctccgt ccacgaaggc tcgggccgtc gtgaggtcgc cctggtccgt
    15061 ctcgatcacg acgacgaacg acgggatctg gcgctcggtc tccactagcc acgccgtgca
    15121 atccgagcga accatctctg ggcaactcga ctccgcgcaa atccggagct cggtgcgcgt
    15181 ctccaggagc tttccgtcca agcgtagctc ttgggatttg ccgtggcttt gcacacacgc
    15241 gtcacgagac gggggcgccg cggccgcttg ggcgctgtac ccgaaaacaa gcgagcacag
    15301 cgccgccgcc agcagcgagt gattcagtcg aggaggtgag tgtcgatgct tcatgctcgc
    15361 ccggtcaagg ttggacggag tgctccacga gagtagcggc caagctagac gggtcgttta
    15421 cggggtcgcg gcagttgata ctcattcagc cggttcacca gggttctgcg ggagatcccg
    15481 agcagctgcg ccgcgcgcgt ttggttgccc tggcacagtc taatcgcctc catcacgcgc
    15541 gaactgtcgc tcaggtgcgc tagaccggcg ccgatgtccc atacgggaac tgtcgtctcg
    15601 gtgtcgctct cccacggatc gatgcccgac gggcggcagc gcagagaagc ggcaatccgc
    15661 ttaccgtcgg gcagatcccg ggggaggatt tcgctgccgc ggcagaggat cactgcgcgc
    15721 tcgaccgcgt tgcgcagttc gcgcacgttc ccgggccagc tgtggttgac caggtgctcg
    15781 accgcttcgc gactgagggt cggttccgcg atgcccccgg agtagcaaaa gcgcttgagg
    15841 aagtggcggg cgagcggctc gatttcgctc ttgcgttgcc ggagcggcgg caggtcgagg
    15901 gaaatggcgt tgatccgaaa gaataggtcg cgccgaaagc gcccttcgct gacctcgacg
    15961 ggcagatcac ggttggtcgc cgccacataa cgcacatcga tggcgcggct gcgcgtagcg
    16021 ccgacccgcg tgacttcgcc cgtttccaag gcgtggagca gctttacttg ggtatgcagc
    16081 ggcagttcgc cgatctcgtc gaggaatacc gtgcccttgt cccctagctc cagcatgccg
    16141 ggcttggctt caccggcgcc agtaaaggcg ccccgttggt gaccaaacaa ttcgctctcg
    16201 agcaacgagt cggagagcgc accacaat
//
LOCUS       DASTKU010000079         7528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1797916, whole genome shotgun sequence.
ACCESSION   DASTKU010000079 DASTKU010000000
VERSION     DASTKU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797916"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFQ61_02660"
     CDS             complement(<1..612)
                     /locus_tag="VFQ61_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953374.1"
                     /translation="MNEVPRSVRVLESYSRWSRVQALLRGGFLAVLLGGVLFSRLAVA
                     QILSPGPLAEGHANLEGDEKCESCHSAGRGISNAKCNGCHTEIAKSEARGTGLHGRKY
                     KGQPCAKCHSDHHGRSFVMVRWEPKRFQHEDAGWPLRGDHASTACNGCHKTKSYIGLS
                     PSCTSCHKDPHESRFGAQCLSCHDEADWNNLRLSQFDHDSSRYP"
     gene            complement(629..1768)
                     /locus_tag="VFQ61_02665"
     CDS             complement(629..1768)
                     /locus_tag="VFQ61_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953375.1"
                     /translation="MRLAGFEDGFVLDLWFAWASRGRGRLFNGRQHPNPSRGAFVFLG
                     AQAPIETENSPHGHQCFVIPDSGAARRLDALGPSFSVGQTEGSITDSTSSLRKIGWLV
                     LGLLTAVAAWALWTRGASFYTTDIHNRVDHPDYRLLSPGHSTGHAYGILATLLVLANL
                     SYLLRRRFPRWRLGSMRLWLDIHVTTGALAGLFALSHSALQMRSPLAVVTMAALGMTL
                     LTGVIGRCIHLFVPHPNHERIAENCSVFESIRPGLGRELYARLHAAPMPEVIGRATLP
                     KVLWLLPSWRRAALYRRRLVLEVLAPYEPYYRAEFHLLSARITETAALAANVPRSVAF
                     DYLLRSWRGLHRFSALLMLVTMVVHVGVAWYYGYRWIFSAASAGH"
     gene            1789..2481
                     /locus_tag="VFQ61_02670"
     CDS             1789..2481
                     /locus_tag="VFQ61_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953376.1"
                     /translation="MHRVFGMLVVALTWVGHFGCDGPNDLDVAHVQVTRHDCAACHLP
                     DYAAARTPLHQDADKALYPTTCGGCHAQSHWSPASFFHPFPLEGRHARAACAQCHTGS
                     PPRFQGTPGECFACHESDYRGSPFPGHSASPTSCGDCHTPSGWSPATGLHPEDLFPIR
                     TGVHQFACRECHDSSRGSNGVDNTNCVGCHVGVHARSRLDPIHLSLELTDYPTGDAPP
                     NFCLSCHPSGSR"
     gene            2492..3835
                     /locus_tag="VFQ61_02675"
     CDS             2492..3835
                     /locus_tag="VFQ61_02675"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9953377.1"
                     /translation="MSPYLFVVWIVLLALLIHLLASHYEARRERALKHQAEELAAVGD
                     VVPPTLHPRIDPLRCIGSGACVNACPEKNVLGVIQGRAHLINAMSCVGHGACATACPD
                     KAIELVFGTRSRGVELPRLDANFQTSQPGIYIVGELGGMGLIRNAILQGAGAAEHIIQ
                     VSNRRAPRLRPETSFDALVVGAGPAGISATLKLMEAGLSVLLVDQEDFGGTILHYPRA
                     KIVMTGDLPLPIYGVVRRSTMPKEKLLELFSELRRRLRIPFQSHQRVVGLEPESEGMW
                     LVRSESHVIRAANVVLALGTRGSPRKLGVPGEELAKVHYRLLEPREFAGRDVLVVGGG
                     NSAVESALSLADAGCCASVALSYRRQHFVRCRGDNRQRIDVAIREGRVRALMPTQVVR
                     IAERHVTITTAQLASGREAQYDLKNDAVIAQLGGTSPADVLKSFGIELMTKYGER"
     gene            complement(3836..6670)
                     /locus_tag="VFQ61_02680"
     CDS             complement(3836..6670)
                     /locus_tag="VFQ61_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET9953378.1"
                     /translation="MLGWLVSCVVALAGCGRWSPPRSVRPEDPGFNDFTAGSIRSLPE
                     FRQLAAVPATIDDRPAIAGAKFVITEFSEPQKRRIRFLDGRQYRFHDEWAWFRLLNGQ
                     PVSGFEPLAPHPFSAPEQARRWALDHEEDLPEGLEITEGRLYARHFYDLSLAKHDRMV
                     GAGSLIHVEGRADRAEIWGFEVDYTDSAEPEQLLVFFSELEKVIPEPARAHLSWFARS
                     PEQLEMGKRLMRLRPELRGRVMTYNQVSVPGETAVYTGGIVAGRLRVVEDLARLGDAE
                     PGDILLLGALPEYLPQARGLVTAVPQTPLAHLNLLAKSRQIVNAYRGGVLEDLELRDW
                     ARSGAPVLLVAEQGRLRFQRLTEREYESYQALLRPEPLGVPYVNTTQLPYIVDLSTVD
                     SKQVPALSTWIGGKAAGMAQLLNSVHGYVAASPLRALRVDVPDRPLVITIRAYREHVA
                     PMLPELRALFADPNFLRYKQLRYLALEGAERFVKRYPGKKNRELARSYQNARALGPIA
                     AVVRKGGVRHMVRHAPLSAELSAELRRVLPRHFGAYSADQGLRFRSSSTVEDIEGFNG
                     AGLYTSNTGFLERDRSSWPVSGKGKRPSVADAVKKTWASYFGAEAFEERHLNHIDHLS
                     ADMAVLVHARFEDTLEQSNGVFTFTWTGSSGELAVDAQPGAVSVTNPPTDRVVIPESS
                     RVRRGRGELVIDRQSLSSLTNAGEQVLKDAELRELFSLAEALTVKHLLADNQGVSPAQ
                     QRRTLVLDFEFRRMAAGWPALQDGSNPARFIVKQMRPLEPSPRVSPELRSAPIPRDVL
                     SRARRIESKLCQGSALEIAIRMVFTEPSGTPDVGYSKVPLVSDVTVKADHGAARVVTH
                     LEQRTANVSSDEIHVEYVSGAAISDVRVDTHQAHVTFADGKRAHSDVRCESQLEYAEP
                     RELLRSFLHASERVRPTRHGASAGEASHER"
     gene            complement(7261..>7528)
                     /locus_tag="VFQ61_02685"
     CDS             complement(7261..>7528)
                     /locus_tag="VFQ61_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953379.1"
                     /translation="AITRVGDDNADGLISPYQVERDAIVDNLNTVLAWLDRERTGRNA
                     ELWMTEGFDDSFRELRGSRQELFERAKEVLLQSVDVPSLRLRFQ"
BASE COUNT         1416 a         2495 c         2318 g         1299 t
ORIGIN      
        1 cggatagcga ctcgagtcgt ggtcgaactg ggacaggcga agattgttcc agtccgcttc
       61 gtcatgacag gagaggcact gggccccgaa ccgagactcg tgaggatctt tgtggcagct
      121 cgtgcaggac ggcgaaaggc cgatgtagct cttggttttg tggcagccat tgcacgccgt
      181 cgaggcgtgg tctccgcgca gcggccagcc cgcgtcttca tgctgaaagc gcttgggctc
      241 ccagcgcacc atcacgaagc tccgcccgtg gtgatcggag tggcacttgg cgcagggttg
      301 acccttgtat ttgcgcccgt ggagcccggt gccccgcgct tccgacttgg caatctcggt
      361 atggcagccg ttgcacttgg cgttggagat gccgcgccct gcgctgtgac agctctcgca
      421 cttttcgtcc ccttcgaggt tcgcgtggcc ctccgcgagc ggaccgggag acaagatttg
      481 tgcgaccgcc agccgggaga agagcacgcc gccgagcagc acggccaaga acccgccacg
      541 aagcaacgcc tggacgcgac tccacctaga ataactctcc agaacgcgca cggaccgtgg
      601 gacttcgttc atctcggtac ggggcgagtc aatgtccagc ggacgcggcc gagaagatcc
      661 accgataacc gtagtaccaa gcaactccaa cgtgcacgac catcgtcacc aacatgagca
      721 gcgccgagaa ccgatgtagc ccccgccacg atcgcagcag atagtcgaac gcgaccgaac
      781 gcggcacgtt tgccgcgagc gccgcggtct ccgtgattcg cgcggacagc aggtggaact
      841 ccgcgcgata ataaggctcg tacggcgcga gcacttccaa caccaagcgt cgccgataga
      901 gcgcagcgcg gcgccagctc gggagaagcc acaggacctt gggcagggtg gcgcggccga
      961 tcacctccgg cattggcgcc gcatggagtc gcgcgtatag ctctcgcccc aagcccggcc
     1021 gaatcgattc gaaaaccgaa caattctccg cgatgcgctc gtggtttggg tggggtacga
     1081 agaggtggat gcagcggcca atgacgcctg tcagtagcgt catgccgagc gccgccatcg
     1141 tcaccaccgc gagtgggctc cgcatttgca gcgcggagtg cgacagtgcg aacaagcccg
     1201 ctagcgcccc ggtcgtcaca tgaatgtcca gccagaggcg catcgaaccg agtcgccagc
     1261 gggggaatcg gcggcgcaag aggtagctca ggtttgcgag tacgagcagc gtcgccagaa
     1321 tgccgtaggc gtgccctgtc gagtggccgg gcgacagcaa gcgatagtcg ggatggtcga
     1381 cccgattgtg gatgtccgtc gtgtagaagc tcgcgccgcg cgtccacagg gcccacgcgg
     1441 cgaccgcggt cagcagtccc agcacgagcc agccgatctt tcgcagactg ctggtcgagt
     1501 ccgtaatcga accttccgtc tggcccacgg aaaacgacgg gcccaacgcg tcgagcctac
     1561 gcgcggcgcc agaatcgggg atcacgaagc attgatgacc gtggggactg ttttccgtct
     1621 cgattggcgc ctgcgccccc aaaaaaacga acgcacctcg agacggattg gggtgctgcc
     1681 ggccattgaa cagtcgtccg cgcccccggg atgcccaagc gaaccacaga tccaacacga
     1741 aaccgtcctc gaacccggcg agccgcatgg ctgccgtggg cgattgattt gcacagagtc
     1801 tttgggatgc tggtcgtcgc gctgacgtgg gtaggccatt tcggctgtga cggtcccaac
     1861 gacctcgacg tggcccacgt ccaagtcacg cggcacgact gcgcggcgtg tcacttgccc
     1921 gactacgcgg ccgcgcgaac gcctctgcat caggacgcgg acaaggcgct ctatccgacc
     1981 acttgcggcg gttgccacgc gcagtcccat tggtccccgg cgagcttctt ccatcccttc
     2041 ccgctcgaag gtcgccacgc tcgcgccgca tgcgcccagt gccatacggg tagcccgccg
     2101 cgtttccagg gcacgcccgg agagtgcttt gcgtgccacg aaagcgacta ccggggcagc
     2161 cccttcccag ggcactccgc gtctccgacg agctgtggag attgccatac gccctcgggt
     2221 tggagccccg cgacgggcct tcaccccgag gatttatttc ctatccgcac cggcgtacac
     2281 cagttcgcgt gccgggaatg tcacgactca tcgcgaggca gcaacggtgt cgacaacacc
     2341 aactgcgtgg ggtgccacgt aggcgtgcac gcccgttcac gcctggaccc gatccacctc
     2401 agcctcgagc tcacggacta tcccacggga gatgctccgc caaacttctg cctgagttgt
     2461 cacccgagtg ggtcacgctg aaggcgcgcg catgtccccc tatctgttcg tagtctggat
     2521 cgtgctcctc gcgctgctca tacacctgct cgcctcgcac tacgaggctc gccgtgaacg
     2581 agccctgaag caccaggcgg aggagctcgc ggcggtcgga gacgtggtcc cgcccacgtt
     2641 gcacccccgc atcgatccgc tgcgctgcat tggttcaggg gcttgcgtca acgcctgccc
     2701 ggagaagaac gtgcttggcg tgattcaggg tcgcgcccac ctgatcaacg cgatgagctg
     2761 cgtcgggcac ggtgcgtgtg cgaccgcttg ccccgacaaa gcgatagagc tggtctttgg
     2821 cacccggtcg cgtggcgtcg agctgccgcg cctcgatgcc aacttccaga ccagccagcc
     2881 cggcatctac atcgtcggtg agctgggcgg catggggttg attcgaaacg cgatcctcca
     2941 gggcgcgggc gcggcggagc acatcatcca ggtgtccaat cgccgagctc ctcgcttacg
     3001 accggagacc tcattcgacg cgctggtcgt cggcgccggc ccggcgggca tcagcgccac
     3061 gctgaagttg atggaggccg ggctcagcgt gttgctggtc gatcaggagg acttcggcgg
     3121 caccatcctg cactacccac gagccaagat cgtgatgacg ggggatctgc cgctgcccat
     3181 ttatggggta gtgaggcgca gcacgatgcc gaaggaaaag ctgttggagc tgttctccga
     3241 gctccggcgc cgcctccgaa tcccattcca gagtcaccag cgcgtggtgg ggctggagcc
     3301 ggaatcggag ggcatgtggc tcgtgcgctc ggaaagccac gttattcgag ctgccaacgt
     3361 cgtattggcg ctcgggacgc gaggctcccc ccgcaagctc ggggtgccgg gagaagagct
     3421 cgccaaggtc cattatcgct tgctcgagcc ccgcgaattc gcaggacggg acgtgctcgt
     3481 ggtcggcgga ggcaacagcg cggtcgagag cgcgctatct ctcgccgatg cggggtgctg
     3541 cgcttccgtg gcgctcagct accgccgcca gcacttcgtg cgctgccggg gcgacaatcg
     3601 gcagcgcatc gatgtcgcca tacgcgaagg tcgggtgcgc gcgctcatgc cgacgcaggt
     3661 ggttcggatc gcggagcgcc acgtcacgat aaccactgca cagctcgctt ccggccgcga
     3721 ggcgcagtac gacctgaaga acgacgcggt gatcgcgcag ctcggcggca cgagccctgc
     3781 tgatgtcctc aagagcttcg gcatcgagct gatgaccaag tacggagagc gctagtcagc
     3841 gttcatgtga cgcttccccg gcgctagcgc catgccgagt gggacgcacg cgctctgagg
     3901 cgtggagaaa gctccgaagc aactcccggg gctccgcgta ctccagttga ctctcacacc
     3961 gaacatcaga gtgagcccgc ttgccgtccg cgaatgtcac gtgggcttgg tgagtgtcga
     4021 cccggacatc cgagatcgct gcgccactca cgtattctac gtgaatctcg tcgctcgaca
     4081 cgtttgccgt ccgctgctcc aagtgggtga cgacgcgagc tgccccgtga tcggccttta
     4141 cggtaacgtc gctcacgagc ggcaccttgg agtagccgac gtcgggggtc ccgcttggct
     4201 ccgtgaacac catgcgaatg gcgatttcga gggcggatcc ttggcaaagc ttcgactcga
     4261 tccggcgcgc gcgagacagc acatcgcgcg gaatgggcgc cgagcggagc tccgggctca
     4321 cgcgcgggct cggctcgagc gggcgcatct gcttcacgat gaagcgcgcc ggattgctcc
     4381 cgtcctggag cgctggccaa cccgcggcca tgcggcgaaa ctcgaagtcc agaaccagcg
     4441 tgcgccgctg ctgcgccggg gaaactcctt gattgtcagc cagcaggtgc ttgaccgtga
     4501 gcgcttcggc caaactgaat aactcccgca actcggcatc tttgagcacc tgttcccccg
     4561 cgttcgtgag agacgataga gattgtctgt cgatgacgag ctctcccctc cccctccgca
     4621 cccgcgaact ctccgggatc accacgcggt cggttggtgg attcgtgaca ctgacagctc
     4681 cgggttgcgc gtcgaccgca agttcacccg agctccccgt ccaagtgaaa gtgaagaccc
     4741 cgttcgattg ctccagcgtg tcttcgaagc gcgcgtgaac cagaacggcc atatccgcgg
     4801 agaggtggtc gatgtgattc agatgtcgct cttcgaacgc ctccgcgcca aagtacgatg
     4861 cccatgtttt cttcacggcg tccgccacac tgggccgttt gcctttacct gagaccggcc
     4921 aggaagaccg atcgcgctcc aagaagccag tgttggacgt atagagcccg gccccgttga
     4981 agccttcgat gtcctcgacg gtgctcgaag aacggaagcg gagcccctga tcggcggaat
     5041 aggctccaaa gtgccgaggc agcacacggc gaagttccgc gctcaactcc gcggagagcg
     5101 gcgcatgccg caccatgtgg cggacgcccc ctttgcgcac cacggctgca atgggcccga
     5161 gagcacgcgc gttctgataa ctacgcgcca actcacggtt cttcttgcct ggatagcgtt
     5221 tcacgaagcg ctccgcacct tcgagcgcga ggtagcgcaa ttgcttgtac cggaggaagt
     5281 tcggatcagc aaacagagcg cggagctcgg gcaacatcgg tgccacgtgt tcccgataag
     5341 cgcgaatcgt gatcacgagc ggacgatcgg gaacgtccac gcggagcgct cgaagtggag
     5401 aggccgcaac gtagccatgc acgctattca agagctgagc catgccggcg gccttacccc
     5461 cgatccaagt cgagagcgcc ggcacttgct tagaatccac ggtcgagaga tcgacgatgt
     5521 acggaagttg agtggtgttt acgtacggta ccccgagtgg ctccgggcgc agcaaagctt
     5581 ggtaagactc gtactcgcgc tccgtcaggc gctgaaaccg gagtcggccc tgctcggcta
     5641 cgagcagcac cggagcgccg ctccgcgccc agtctcgcag ctccaggtcc tccagcaccc
     5701 ccccgcgata ggcgttcacg atttgacgac ttttcgcgag caaattcaag tgcgcgagtg
     5761 gcgtctgcgg aaccgcggtg accagtccac gagcttgtgg cagatactcg ggcaacgcgc
     5821 cgagcaatag gatatctcca ggctccgcgt cgcctagccg ggcaaggtct tccaccacgc
     5881 gcagccgccc ggccacgatt ccgccggtat agacagccgt ctcgcccggc acgctcacct
     5941 ggttgtaagt catgacccgg ccgcgcagct cgggacgtaa gcgcatcaac cgtttgccca
     6001 tttcgagttg ctcgggcgag cgggcaaacc agctcaagtg ggcgcgggcc ggctctggaa
     6061 tcactttctc gagctcagaa aagaagacca gcagctgctc tggctccgcc gagtccgtgt
     6121 aatcgacctc gaagccccaa atttccgctc gatcagctcg gccctccacg tgaatcaggc
     6181 tgccggcgcc gaccattcga tcgtgtttgg ccaagctcaa gtcataaaag tgtcgcgcgt
     6241 acaagcgacc ttcggtgatc tccagaccct caggcaaatc ctcttcatgg tcgagcgccc
     6301 agcgccgcgc ctgctccgga gcagagaagg gatgcggcgc gagcggctcg aatccggata
     6361 ccggctgccc attgagcaga cgaaaccaag cccactcatc gtgaaaccga tactgacgcc
     6421 catccaagaa gcgaatcctg cgcttttgcg gctcggagaa ctccgtgatc acgaacttgg
     6481 ccccggcaat cgcggggcgg tcgtcgatcg tggccggaac agcggcgagc tgtctgaact
     6541 caggcagcga ccgaatggaa ccggccgtaa aatcgttgaa accaggatcc tcgggccgaa
     6601 cgctcctcgg cggtgaccag cgcccgcagc ctgccagcgc caccacgcaa gagaccaacc
     6661 aacccagcat ccgacaccaa gagagtactg aagaacgggc caccacgctg atggacgctc
     6721 gaacgtacga aaagcttgaa cctttcagta cggcttgtgg cttttcgagc gccttgccgt
     6781 gcccggccta cctacaggac cctcggctta caggaccctc ggccacgcgc agcgtaggct
     6841 cggcggagcg agcatggcct tgagggcctc gggccgcgcg cagcgaacgc gagcacggac
     6901 cggcctttcc ccgtcgctct attgtcgggg gagcaacact cggtggtact cagcagccgt
     6961 ggccgtagtt cttgatcacg gtcgcatagt cgttcaccac cccatcgccg tttacgtcgg
     7021 cgagcacgct ctggttgcac gggttcggcg acgctgccgc cctccgcacc ggctggcatt
     7081 ccaccctcgg agctgccgcc cgtgttctcg gaaactcccc cggtgccgcc gccatcctgc
     7141 gcgccagagc gcccgttgcc accgccacaa gccagcacca acacgaacac aacgtacgct
     7201 ctcgatctca tcttctttct cgtagacaag gcaagctgac gtgctgcgct gggttaccca
     7261 tcactgaaac ctaagccgca gcgacggcac atcgacgctc tgcagcagca cctctttcgc
     7321 ccgctcgaag agttcctgcc gcgagccccg tagctctcga aaggaatcgt cgaatccctc
     7381 ggtcatccac aactccgcat ttctaccagt ccgttcacga tcgagccacg caagcacggt
     7441 attcagattg tccacgattg cgtcacgctc gacttgatag ggcgatatca gcccatccgc
     7501 gttgtcatca cccacacgag ttatagca
//
LOCUS       DASTKU010000080        14011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_236107, whole genome shotgun sequence.
ACCESSION   DASTKU010000080 DASTKU010000000
VERSION     DASTKU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14011
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236107"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VFQ61_02690"
     CDS             <1..233
                     /locus_tag="VFQ61_02690"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET9953380.1"
                     /translation="EQKLQSSALDEALQSLPARQAAALTLVHLDGLSGLEAAHVLGVS
                     AEALESLLSRGRRSLRKRLTKEPVINSEEDFK"
     gene            230..715
                     /locus_tag="VFQ61_02695"
     CDS             230..715
                     /locus_tag="VFQ61_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953381.1"
                     /translation="MSRRRASALSEERFQSLLEAYGARLAAWPEAEREAVEALLESSA
                     SARDALAREADLDRALFSASVDPALSTSLERRLAELPVRHPRPAGKDAQSALSHWLKR
                     WFWAPALGFSALTLLAVVLGSRVDEAATAVPEEDVVTASDSLALELALGVAPEFEEGE
                     P"
     gene            712..1128
                     /locus_tag="VFQ61_02700"
     CDS             712..1128
                     /locus_tag="VFQ61_02700"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HET9953382.1"
                     /translation="MTPLAKKLLIALIVSVGVNLALLGFLAGRAFRPFPGPRPVSGPT
                     GPGPRPGFFAGPPAELRARLHETREARARVAEALEHEPFDTATLESALRALRTETTQG
                     QETLHAELVRRAGSATPEARRALAGQFRRGRFWKGK"
     gene            1252..2694
                     /locus_tag="VFQ61_02705"
     CDS             1252..2694
                     /locus_tag="VFQ61_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953383.1"
                     /translation="MVRLRSQAATRAAAAALLGWNSILTCACSGGAERALPSEYPVFL
                     EGGVAGKHGALLSGAAGGSGGTANGTPPGENTTGGTSWLLPRAGSNGTGSTPEPGCSS
                     VSARAPIELRPVDIVFVIDNSSSMNDEIAAVQTRINTDFAAQIANSGLDYRVVMISRY
                     GKVGVPVGESDNPICIAPPLGKSGCEDPAHTQLENGERFKHFSADIESHDALCALLGG
                     FSESDEYADVARRGWRPLAPTGYSQYLRPEAFKHIVVISDDDVSCQFGSLELDDHSSA
                     MGGEAVATAFDRALLKLSPAQFGTPEHRNYAFYSIVGLAENKDPVRAYAASEPITTST
                     CGSGSAGPGTGYQALSRLTSGLRYPSCRSQNFDAIFSALASGVVSQSRMACRFELPRR
                     PDGLAFDPSELNVQFRASELAPPEEILHVSSSKACATTAGSSSEGWYYDDALAPTALQ
                     LCPATCARVEPQVAGQLDLLFGCHTHEAPR"
     gene            2819..5338
                     /locus_tag="VFQ61_02710"
     CDS             2819..5338
                     /locus_tag="VFQ61_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9953384.1"
                     /translation="MATAPESDVTPAAVSTARGRPNIGVFIDDLSNGYTDPILHGMSE
                     RARARDVNLVTFVGSVHPEDIGGKRHLTSDLASEHSVDGIVVITLGQTLSAAELGAYC
                     ERFRPLPMCCVPDVEADFCSRLWVDGRSGMRDAVRHMIEVHGYRDFAFMKGPARSSEA
                     EARYEAFRETLRSFDVELDEACVVLGDYSVAGGVAAAEELFDRRGRRPRALVAANDGM
                     AVGALKVLARLGFSVPDQVAVMGFDDIEYARYTDPPLSTVRQPLRQLGQDALEMVLSR
                     IEGAAWVEERVLASGLVVRESCGCFGQRELAPLLDARATSGPQLSSLGPAIAREIRKL
                     GIPGTQQTGFTERLVSAFLAEFAAPRRESFLRELRTILEAVTQLRGDPGGFQNAISIL
                     WQATQASVDVRSDAFRLADWLLHQSRIMVSVHAERAQSLRQARLGDFAAKLLGSSASL
                     GLSVDVESLANAMAGAFPTYGIESCQVYAYGTSIPGSRDARFLSGFMGRRSMARSGEP
                     ENDDAARLLARELAEITARAEGPRRYLIGPLRRVGDSPGFALFTEGPYDGFVYENLLD
                     QIANAVSRIQLLDHLVEEAERRETAERQRLERELALAQHIQLGILPRHVHVEGLEIAS
                     TMLTATEVGGDYYDIVPTGTGCWFCIGDVAGHGLPAGLVMLMLQSVVAGLLYDSPAAQ
                     PREVLPIVNQVLFENVRQRLRQDEYATLNLLRYDADGQLTFAGAHEDILIYRVRTGRV
                     ERLATLGTWVAATRDIREASIDTRAWLDVGDVMLLYTDGVIEARNSAGQDFGLDRLAD
                     LFESLGSVPASEIVARLREAVTTFQREQEDDLTLLAVRRLS"
     gene            complement(5419..5793)
                     /locus_tag="VFQ61_02715"
     CDS             complement(5419..5793)
                     /locus_tag="VFQ61_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953385.1"
                     /translation="MDEPTLPKLAQSGFSLVPELSESALLLQFAGSAELAAAEPIRNY
                     LSAAHEVAIRRGLAEVRVDLRALKFMNSSCFKSFVTWLDTVANHETGYRVRFLANAEL
                     HWQRRSLESLRRLAAHVVSVEF"
     gene            6082..10266
                     /locus_tag="VFQ61_02720"
     CDS             6082..10266
                     /locus_tag="VFQ61_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9953386.1"
                     /translation="MRRPLRLGILIDSLLSTYQIRLFHGARRAARRHGAHLVGFQGSF
                     LRDRAPTPTGFDGSFLYELARPEAVDGLVVVSSILSSEIGLEGVRDLCAASLLPVVSV
                     EELPGFPYIGVESRQGLRTLLEHLITVHQRRHFVFIRGNNPDSRQREATFRETLLEHG
                     LPVIEDLILDGTFLEVSGGLAIRTLFDERHVPSWGVDAVVAGNDQMAAGAIAELEKRG
                     LRVPRDVSVVGFDDDEHSINYSPPISTVYQPVEGVGEAAVDALVERIHGREVPPARRL
                     PTRAVIRRSCGCERTRRPNKSWHFVDESVEDRLRARGAELLAQLEEHSVSPDLASSVE
                     RLLDALCAETDAQFEQGLVGLEQTLSVASNTGLDALRFQEAVEPLMAALETARGPAKQ
                     AEERVAQRLAQARMVAMEVAVRTRTRSRLRALQQATALRVLGSTLACARNLSGVVRGL
                     KLGLPSFGVRYCCVCLFVPESQRRLARLLAQYEVNGGPPASSTPDLLTEPSQVWRSLP
                     SEPPLEGAETHDIPIFSSADLVPLDRSPSEAHLLLYPLVFAEAALGYVVFDAPLEDDR
                     IWLLESIAGHLSSALYALLRSDELKLAREQAEQASAAKSEFVAVMSHEVRTPLTAILG
                     HVDLCLRTTLNREQREHLQRAKTSSQALIGIVDDILDFSKIEASRLELERVQFELDDV
                     LEQLIGTCSMAAARKGLELSVDVDPELPRCLVGDPLRVTQILLNLVGNAIKFSDTGYV
                     ALRVKCTALRDRQVLLEFAVQDTGIGMTEAELSRLFRPFTQADSSTTRRYGGTGLGLT
                     ICKRLVDMMGGTLQVSSEPGRGSTFAFAVEFGIASMPETLMPQGAGLHVLLVEDSEPQ
                     REALQRLLRAHAYTVHAASNATQGLALLRALEERGQALHLAVLDYGLPDGDGLSLAQR
                     IAAQAGGAATLGIVLLIPPTADRMIAESSRRLEVAAAIGKPFLRKNVLRALSKARRNS
                     TSSMPPSTNSLPEGRLSGLNLLLVQDNEVSLEVSRAVLELQGANVLTAADGAEALQRA
                     ADRDFDAVLMDLQMPVLDGCAATRALRQTSRHAKTPILALTASTNSRDRTRASEAGMD
                     GFLTMPLEPRELAAKIREALRGPNEAATPNNAEPGARTLEFATHSTLPRLELDMTSAL
                     ARLGGDTQAFRKLLTRFERTYSSLSSQFDRAIHDGDSRAVGMLAHTLSSAAANIGANR
                     LHHSARALETALERNGLLSVGAMMAELLAALDSTLHAARTVLDTTPWHRRTPSGQFHL
                     DPLPVLSHMRKLLEDHDTAAVECLESLRSGVADRLNAAEPLRRLETSIESYDFEQARR
                     ELETLERLLSDFDSHFPPSPDTAGSTGPAGSTDTTPLVTGETTAAGGASTSAQTNAVP
                     SQLSAGNNNQHERDSE"
     gene            10247..11275
                     /locus_tag="VFQ61_02725"
     CDS             10247..11275
                     /locus_tag="VFQ61_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HET9953387.1"
                     /translation="MNATLNKPVVLVVDDAPESIDVLRGVLGAEYQVKAAILGPLALE
                     IAENAPPDLILLDVMMPGMDGYEVCRRLKSRPSTAKIPVIFVTTLSDVGSETRGLDLG
                     AVDYVTKPYVPSLVRSRVRTHVTLHQNNQRLEALVQDRTRELLETRVEIIRRLGRAAE
                     YRDNETGMHVLRMSHIARLIALSVGLGEAESELILHASPMHDIGKIGIPDRILLKPGP
                     LDPEEWEMMKRHTVIGAEILGDHPSELIRMARLVALRHHERWDGKGYPDGLRGEQIPI
                     AARVVAIADVFDALLSVRPYKRAWSVEDTLAKLSAESGHHFDPTVVNAFLRILPECLE
                     VRARYRDD"
     gene            11344..11781
                     /locus_tag="VFQ61_02730"
     CDS             11344..11781
                     /locus_tag="VFQ61_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9953388.1"
                     /translation="MLDVGIRKAFASFLVSGALALGATGCGRAAGATPAELGKGRDAS
                     GTGALLYERNCQSCHGEQGQGTGKAPAILGRRALASGRFRNAEKLFDYVARKMPADEP
                     GRLDIGQYWNIVTFMVATGGHRIPDGRLSEANAAKIKLIDDDD"
     gene            complement(11849..13426)
                     /locus_tag="VFQ61_02735"
     CDS             complement(11849..13426)
                     /locus_tag="VFQ61_02735"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9953389.1"
                     /translation="MSRLGFASVAPAPGSRDSLIDSEEEGRAPIVEPQSESGWNAAPD
                     AGTRLDVAEGAAAERNGQARISHAAGPISVRAPIAKIGRYALFDKFATGGMATVHLGR
                     LDGAGGFSRVVAIKRLLPHLILNQEFAEMLLKEARLAARVRHPNVVPTLDVVALKHEV
                     LLVLEYVHGESLSALCRAQSKLREWIPVPIVASVMIGALHGLHSVHEATDEKGQLLGL
                     VHRDVSPPNVLIGTDGIARVLDFGIVKALEQMEETVPNRLKGKTGYMSPEQIRGERLT
                     RSSDIFSAGIVLWELLTLRRFAPGPSDKQRLDRIARGNYQKPSEYRPSLPKAVDEVVM
                     RALAFRPQDRYPTARDFAEALGAAVEVASPGPVADWIARLAQESLAERARLLAQVENW
                     DDQAHPAETSAAESVALPLANGAGRGNGASSRASLASSKEDSNESEKSEAAGKGEKPP
                     SSKRERGKGNGKAKADPDDASTSGRGGGSRGGGARAAQPSFAHLIDESKSRSSSFAPF
                     AIVLVVLLLVIVYFSTR"
BASE COUNT         2526 a         4442 c         4591 g         2452 t
ORIGIN      
        1 tggagcagaa gctgcagagc agcgcattgg acgaagcgct gcaaagcctg cccgcgcggc
       61 aagccgccgc gctcacgttg gttcacttgg atggcctatc tgggctcgag gccgcacacg
      121 tcttgggcgt gagcgccgag gccctggagt cgctcctttc gcgtggacgc cgaagcctgc
      181 gtaaacggct caccaaagag cctgtcatca attccgaaga ggatttcaaa tgagccgccg
      241 tcgcgcctcc gcgctttctg aagaacgctt ccagagcctt ctggaagctt acggagctcg
      301 cctcgcggcg tggccagaag ccgaacgaga agcggtcgag gcacttttag agagctcggc
      361 gagcgcgcga gatgccttgg ctcgtgaagc cgacctggat cgcgcgttgt tctcggccag
      421 cgtcgacccc gcgttgtcga ccagcctcga gcggcgtctc gccgagttgc ccgtgcgtca
      481 cccgaggcct gctggaaaag acgcgcagag cgccttgagc cattggttga agcgctggtt
      541 ttgggcgccg gcgctaggtt tttctgcgct cacgttgttg gccgtagtct tgggtagtcg
      601 cgtcgacgag gcagcgactg ccgtgcccga agaagacgtc gttacggcgt cagacagctt
      661 ggcgctcgag ctcgcgctcg gtgttgcgcc cgagttcgaa gaaggtgaac cgtgactccg
      721 ctcgcgaaga agttgttgat cgcgctgatc gtatcggtcg gcgtcaactt ggctttgtta
      781 ggatttctgg ccggccgcgc attccgacct ttccctgggc ctaggccggt gagcggccct
      841 actggtcctg ggcctcgtcc aggattcttt gcagggccgc cagccgagtt gagagcacgt
      901 cttcacgaga cccgtgaagc tcgcgccaga gtcgccgagg cactagagca cgagccgttc
      961 gacacggcga cattggagag tgcgctgcgc gctttgcgca cggagacgac tcagggtcag
     1021 gagacactac acgccgaact cgtgcgccgc gccggctcgg caacgccgga ggcacgtcgt
     1081 gccctcgcgg ggcagtttag gcgcggtcgg ttctggaaag ggaaatgagg cgaagcgtcg
     1141 acttggtgtg gagacccaag tgacggcgag tgcgagaaac acagttcgaa cgctaccaat
     1201 ctggagtgag agtctttcga cgcgtccgcg attgaccgct gttgcgggtg aatggtgcgt
     1261 ttgcggagcc aagccgccac gcgcgcagct gccgcggccc tgcttggctg gaattcgatt
     1321 ctgacctgcg cgtgctcggg aggtgccgag cgtgcgttgc cgagtgaata cccagtcttt
     1381 ctcgagggcg gcgttgcggg gaagcacggc gctcttctca gtggcgccgc tggggggagc
     1441 ggaggcacag cgaacggcac tccacccgga gagaacacga ccggaggtac cagttggctg
     1501 ctgccccgcg ccgggagcaa cggtacgggg agcaccccgg agccggggtg ctcatccgtc
     1561 agtgctcgag ctccgatcga gctgcgcccc gtcgacatcg tgttcgtgat cgacaactcg
     1621 tcgagcatga acgacgaaat cgcggcggtt cagacgcgca tcaacaccga ctttgccgcc
     1681 cagattgcga acagcggact ggattaccgc gtcgtgatga tttccaggta tggcaaggtc
     1741 ggagtgccgg tcggggagag cgacaatccc atctgcatcg cgccgccact cggcaagagc
     1801 gggtgtgaag atcccgcgca cacgcagctc gagaacgggg aacgcttcaa gcacttcagt
     1861 gcggacatcg agagccacga cgctctgtgc gctctgctcg gaggcttctc tgagagcgac
     1921 gaatacgccg acgtcgcgcg ccggggctgg agaccgctgg cccccactgg atattcacag
     1981 tacctgcgtc cagaggcatt caaacacatc gtggtcatta gcgacgacga cgtcagctgc
     2041 cagtttggca gcctcgagct cgacgaccac tcgagtgcga tgggcggcga agcggtcgcg
     2101 acggcgttcg atcgcgcgct attgaagctg agcccggctc aattcggaac ccccgaacat
     2161 aggaactatg ccttctacag catcgtcggg ttggcggaga acaaggaccc ggtgcgcgcc
     2221 tatgccgcga gcgagccgat cacgaccagc acatgcggaa gtggctctgc cgggccaggc
     2281 acgggttatc aggccctcag ccgtctgact tcggggctcc gatatccgtc atgccgatcg
     2341 cagaacttcg acgcgatctt ttcggcgttg gcgagcggag tggtctcgca atccaggatg
     2401 gcgtgccgct tcgagttacc tcgacgcccg gatggtttgg cgttcgatcc gagcgagctc
     2461 aacgtacagt tcagggccag cgaactcgct ccccccgagg agatcctaca cgtgagctct
     2521 tcgaaagcat gtgcaacgac cgcgggctca tccagcgagg gctggtacta cgacgatgcg
     2581 ctcgcaccga cggccctaca gctctgcccc gcgacgtgcg ctcgagtgga gccccaagtc
     2641 gctgggcagc tcgacctact gttcggctgc cacacgcacg aagccccccg ttagaaccgc
     2701 ctgcgcaaga gtttgccaaa gccgcgcggt cgcgcgccgg gtcacctagt gtgggctcac
     2761 tctgcacacg cgattcgaga cttgtcctgc gacgagcatc gggctttagt gggaccgatt
     2821 ggctacggcc cctgaaagcg acgtgacgcc cgcggcagtg tccactgcac gcgggcgtcc
     2881 gaacatcggc gtcttcatcg acgacctctc gaacggttac accgatccga tcctgcatgg
     2941 gatgtccgaa cgagcccgcg cccgcgacgt gaacctcgtg acgttcgtgg gctcggtgca
     3001 cccggaggat atcgggggca agcggcactt gacgtcggac ttggcgagcg agcattcggt
     3061 cgatggcatc gtagtcatca cacttgggca aaccctctcg gcggccgagc tcggggccta
     3121 ttgtgagcgg ttcagaccgc tgcccatgtg ctgcgtgccg gacgtcgagg ccgatttttg
     3181 ctcccgactc tgggtcgatg gtcgctcggg catgcgagac gccgttcggc acatgatcga
     3241 ggttcacggc taccgcgact ttgcgttcat gaaggggccc gcgcgaagca gcgaggccga
     3301 ggctcgctac gaggcgttcc gcgaaacact acgcagcttc gacgtggagc tcgatgaagc
     3361 gtgcgtcgtg ctcggtgact acagcgtggc cggcggcgtt gcggcagcgg aggagttgtt
     3421 cgaccggcgt gggaggcggc cgcgcgcgtt ggtggccgcc aatgacggga tggcggtcgg
     3481 cgcgctcaag gtgctcgctc ggctaggctt ctcggtgccc gatcaggtcg cggtcatggg
     3541 cttcgacgac atcgagtacg cgcgatacac cgatccgccg ctctccaccg tacggcaacc
     3601 gctgcgacag ctcggccaag acgccctcga aatggtgctg tcacgcatcg aaggcgccgc
     3661 gtgggtagag gagcgcgttc tggcgagcgg actcgtggtg cgtgaatctt gtggctgttt
     3721 cggacagcgc gagctcgctc ccttgctcga cgcgcgcgcg acctcggggc cccaactctc
     3781 gagtctcggc ccggccatcg cccgcgaaat ccgcaagcta ggcatccccg gcacgcaaca
     3841 gaccgggttc acggaacggc tggtgagcgc gttcctcgct gagtttgcgg cgccgcgccg
     3901 tgaatctttc ctgcgcgagc tgcgcacgat tctggaggcg gtcacgcaat tgcgcggtga
     3961 tccgggtggc ttccaaaacg cgatctcgat cctctggcaa gctactcagg cgagcgtaga
     4021 cgtacgaagc gatgcgtttc gactcgcgga ttggttgctg caccagagcc gaatcatggt
     4081 gagcgtgcac gcggagcgag cgcagtctct acgccaagcg aggttgggtg acttcgcggc
     4141 aaagctgctc ggcagcagcg caagcttggg tttgtcggtg gatgtcgaat cgctggcgaa
     4201 cgcgatggcg ggagcttttc ccacgtacgg catcgagagc tgtcaggtgt acgcttatgg
     4261 cacctcgatc ccgggctcac gggacgcccg tttcttgtcg gggttcatgg ggcggcgcag
     4321 catggcccgg agcggcgaac ccgaaaacga cgacgccgct cggctcctgg cgcgagagct
     4381 ggccgagatc acggctcgtg cggaaggccc cagacgctat ttgatcggtc ccttgcgaag
     4441 ggtcggcgac tctcctgggt tcgcgttgtt cacggagggc ccctacgacg gcttcgttta
     4501 cgaaaacctg ctcgaccaga tcgcgaacgc cgtcagtcgc atccagctgc tagaccatct
     4561 ggtagaagaa gccgagcggc gagagacggc cgagcggcag cgtttagaac gcgagctcgc
     4621 gctggcacag cacattcagc tcgggattct gccgcgccac gtccacgtcg aaggcctcga
     4681 aatcgcgtcg acgatgctca ccgcgacgga ggtgggtggt gattattacg atatcgtgcc
     4741 gaccggtacg ggctgttggt tctgcattgg agacgtggcc ggccatgggc tgcccgcagg
     4801 tctcgtgatg ctgatgctgc agagcgtggt cgcaggcctg ttgtatgaca gtcccgccgc
     4861 gcagccgcgc gaggtgctcc ccatcgtcaa tcaggtgttg ttcgagaacg tgcgccaacg
     4921 tctgaggcaa gacgagtatg caacgctgaa cctgctgcgg tacgacgccg atgggcaact
     4981 cacgtttgcc ggcgcgcacg aggacatttt gatttaccgc gtccgcacgg gtcgggtcga
     5041 acgcctggcg acgctgggca cctgggtagc ggcgacgcga gacatccgcg aagcgagcat
     5101 cgacacgcgc gcatggctcg acgttggtga cgtgatgctg ttgtacaccg acggggttat
     5161 cgaggcgcgc aactcggccg ggcaggattt cgggctcgat cgattggcag acttgttcga
     5221 gagcctaggg tcggtccccg cgagcgagat cgtggcccgc ctgcgggaag cagtgactac
     5281 ctttcagcgc gagcaagagg atgatctcac gctactcgcc gtgcgacgcc tgagctaggg
     5341 ctacggcgcg acatcgcgca catccggcag cggtacgccg tggcggcagg tgacggcgtg
     5401 aacgcgccga aagcaggctc agaattcgac gctgaccacg tgcgcagcta ggcgcctgag
     5461 cgactccaga ctgcggcgtt gccagtgcaa ctctgcgttg gccaagaatc ggactcgata
     5521 gcccgtctcg tggttggcga ccgtatctag ccacgtcacg aaggacttga agcagctcga
     5581 gttcatgaac ttgagcgctc gtaaatcgac gcgcacctcg gccagcccgc gtcggatggc
     5641 gacctcgtgg gcagcgctca agtagttgcg aatcggttct gccgccgcga gttccgcgct
     5701 cccagcgaac tgcagcaaca gcgcgctctc cgagagttcg ggaacgagcg agaagcccga
     5761 ttgggccagc ttcggcagtg ttggttcgtc catgagcccg cgacgtcacg cagccatgta
     5821 catgccgcgt acggaattca agggtgatac gtacgagtgc ctgatttgat ctgcgatcga
     5881 aacggcaaag ttcaatggaa gcaaaagtgc gcaggtccag attgggctga catgggccgc
     5941 ctccggtcgg cgcgtgtagt ctcgtgtggt ctgactctag ccgacgagaa ggcgacaggc
     6001 gcgagcaggt gagtcgcagc cccaaaggcg cagattaatg gtccgccccg cttggtcgtt
     6061 gccctgttgc acaaacgacc catgcgacga ccgctccggc tcggaatctt gatcgacagc
     6121 cttctgtcaa cctatcaaat ccggcttttt cacggagcgc gccgcgcggc gcgccggcat
     6181 ggtgcgcacc tcgtagggtt ccagggtagc ttcctgcgtg accgagcacc gacgccgacc
     6241 ggcttcgacg gctcgttctt gtacgaattg gcgcgccccg aagcagtcga tgggctcgtg
     6301 gtggtgtcca gcatcttgtc tagcgagatc ggactagagg gggttcgcga tttgtgtgcc
     6361 gcgagcctcc tgccggtggt gagcgtcgag gagctgcctg gttttcctta catcggggtc
     6421 gagagccggc aagggctccg cacgctgctg gagcatctca ttacggtgca tcagcgtcgc
     6481 cacttcgtct tcatacgtgg caacaatccc gacagtcgcc aacgtgaggc gacgtttcgg
     6541 gagacgctgc tcgagcatgg gctgcccgtg atcgaggacc tcatcctcga cggaaccttc
     6601 ctcgaagttt cgggcgggct cgccatcaga acgttgttcg acgagcgcca cgtgcccagt
     6661 tggggcgtcg acgcagtcgt agcgggcaac gatcaaatgg cggcgggcgc gattgccgag
     6721 ctcgagaagc gcggactgcg agtcccccgt gacgtctcgg tcgtcggctt cgacgatgac
     6781 gaacactcca tcaactacag cccgccgatt tcgacggtat accagcccgt cgaaggggta
     6841 ggagaagccg ccgtcgatgc gctggtcgag cgtattcacg gacgagaagt cccccccgcc
     6901 cgcaggcttc ctacgcgggc cgtaattcgg cgctcctgcg gctgcgaacg cacgcgacgg
     6961 ccgaacaaaa gctggcactt cgtagacgag agtgtggaag atcggctccg cgcgcgcggt
     7021 gccgagttgt tggcgcaact ggaagagcac agtgtcagcc ccgacttggc gagcagcgta
     7081 gagcgactgc tcgatgcctt gtgcgcggag actgacgccc agttcgagca aggtttggtg
     7141 gggctcgaac aaaccctgtc cgttgcgtcg aacacgggcc tggacgcgct gcgttttcag
     7201 gaggccgtcg aaccactgat ggccgcgctc gaaacggcgc gagggcccgc caagcaggcc
     7261 gaagagcgcg tcgcccagcg actcgctcaa gcgcgcatgg tcgcaatgga ggtcgcggta
     7321 cgaacacgca cgcgcagccg cttgcgagct ctgcagcagg ccaccgcgct gcgggttttg
     7381 gggagcacct tggcctgcgc gaggaacctg tcgggggtag tgcgcggctt gaagctgggg
     7441 ctcccgagct tcggggttcg ctactgctgc gtgtgcctgt tcgtaccgga gagccaacga
     7501 cgcctcgccc gcctgctcgc gcagtacgaa gtgaacggcg ggccacccgc gtcgtccacc
     7561 cccgacttgt tgaccgagcc gtcacaggtg tggcgtagct tgccgagcga acctccgctg
     7621 gaaggcgccg agacccacga catcccgatc ttctcgagtg ccgatctggt gccgctcgac
     7681 cgatccccga gcgaagctca cttgctgctc tacccgctcg tgtttgccga ggccgcgctc
     7741 gggtacgtcg tgttcgacgc gcccctggag gacgatcgta tttggctgct cgagagcatc
     7801 gccggtcacc tgagcagcgc gctttacgca ctattgcgct cggatgagct caagttggcc
     7861 cgcgaacagg ccgaacaggc cagcgccgcc aagagtgagt tcgtagccgt gatgtcgcac
     7921 gaggtccgca caccgctgac cgcgatcttg ggtcatgtgg atttgtgcct gcgcaccacg
     7981 ctgaaccgcg agcagcgaga gcacctgcag cgcgccaaga cttcatcgca agcgctgatc
     8041 ggcatcgtgg acgacatcct cgacttctcc aagatcgagg cctcgcggct cgagctcgaa
     8101 cgcgtgcagt tcgagctcga tgatgtgctc gagcagctga ttggaacgtg ctcgatggcc
     8161 gccgcccgca agggcctcga gctcagcgta gatgtcgacc cagagctgcc gcgctgcttg
     8221 gtcggtgatc cgctgcgtgt cacccagatc ttgttgaacc tggtgggcaa tgccatcaag
     8281 ttctcggaca cgggttacgt cgcgctgcgc gtcaagtgta cggcgctgcg cgatcggcag
     8341 gttttgctcg agttcgccgt tcaagatacc ggcatcggca tgaccgaggc cgagttatcg
     8401 cggttgttcc ggccattcac gcaggccgac agctccacca ctcgtcgcta cggcggaacc
     8461 ggactcgggc tcacgatctg caagcgtctg gtggatatga tgggcggcac gctgcaggtt
     8521 tccagcgagc cgggccgcgg cagcacgttt gctttcgccg tggagttcgg aattgcttcg
     8581 atgcccgaga cgttgatgcc gcagggcgcc gggctccacg tgctgctcgt ggaggatagc
     8641 gagccgcagc gcgaggcact ccaacgcctg ctccgcgccc acgcctacac cgttcatgcc
     8701 gcgagcaacg ccacgcaagg cttggcgctg ctgcgcgcgc tggaagagcg aggacaagcg
     8761 ctgcacctgg ccgtgctgga ctacggttta ccggatggcg atgggctgag cctcgcgcaa
     8821 cgcatcgcgg cgcaggcggg aggcgccgcg acgctcggaa tcgtcttgct gatcccgccg
     8881 acggcggacc gtatgattgc agagagctcg cgacgcctcg aagtggcggc cgcgatcggc
     8941 aagcccttct tgcgcaagaa cgtgctccgc gcgctgtcga aagcgcggcg caactccacg
     9001 tcgtccatgc ctccgagcac caacagcctg ccggagggca ggctgagcgg gctcaatctg
     9061 ctgctcgttc aggacaacga agtgagcctg gaagtttcgc gcgcggtgct cgagctgcag
     9121 ggggcgaacg tgttgacggc cgccgacggc gccgaggccc tgcaaagggc cgcagatcgt
     9181 gatttcgacg cggtgctgat ggatctgcag atgccggtac tcgacggctg cgccgcgacg
     9241 cgcgcattgc gccaaacctc gcgccacgcg aagaccccaa tcctcgcgtt gaccgcgagc
     9301 acgaactcgc gagaccgaac gcgcgcgtcc gaagccggga tggacggctt cttgaccatg
     9361 ccgctcgagc cacgcgagct cgcggcaaag attcgagagg cattgcgagg ccccaacgaa
     9421 gcggcgacac ccaacaacgc ggagcccggc gcacgcacgc tcgaattcgc cacccattcg
     9481 acgctgccgc ggctcgagtt ggacatgacc tccgcgttgg ctcgcctcgg cggcgacacc
     9541 caagcctttc gtaagctgtt gacgcgcttc gagcgcactt actcgagcct gagcagtcaa
     9601 ttcgatcgag ccatccacga cggcgacagc cgcgcggttg gcatgctcgc gcacaccttg
     9661 tcctcggcgg ccgcgaacat cggagcgaac cggcttcatc actccgcaag agccctggag
     9721 accgccctcg aacgtaacgg gttgctcagc gtgggcgcca tgatggcaga gcttttggcg
     9781 gcgctagaca gcaccctgca tgccgcgcgc accgtgctcg atacgacgcc ttggcaccgg
     9841 cgtactccga gcggtcagtt ccatctcgat ccgttgccgg tgctgtcaca catgcgcaag
     9901 ctgctggaag accacgatac tgccgccgtg gagtgcctcg agtctctacg cagcggcgtg
     9961 gccgaccgct tgaatgctgc ggagccgctg cgccggctcg agacgagcat cgaatcctac
    10021 gacttcgagc aggcccgccg cgagctagag acgctcgagc ggctgctctc cgatttcgac
    10081 agtcacttcc ctccttcgcc ggacaccgca ggctcaacgg gccccgcagg ttcgaccgac
    10141 accacgccgc tggtcacggg agagaccacg gccgcgggcg gagcctcgac ctccgcgcaa
    10201 acgaacgcgg taccaagcca gctttcagcg ggaaacaaca accagcatga acgcgactct
    10261 gaataagcca gtagttttgg tggtcgatga cgcgcccgag agcatcgacg tactgcgagg
    10321 ggtgctgggg gccgaatacc aagtcaaggc cgcaatcctg gggccactcg cactcgagat
    10381 cgcggagaac gcgcccccgg atctgatcct gctcgatgtg atgatgccgg gcatggatgg
    10441 ctacgaggtc tgtcggcgcc tgaagagcag gccgagcacc gcaaaaatcc ccgtgatctt
    10501 cgtgaccact ctttcggacg tggggagcga gacccggggc ctcgacctgg gcgcggtgga
    10561 ttacgtcacc aaaccgtacg taccgtcttt ggttcgctca cgagtgcgca cgcacgtgac
    10621 gctgcaccag aacaaccagc gcttggaggc cctggttcaa gatcgcacgc gcgagctcct
    10681 ggaaacccgc gtcgagatca ttcgccgctt gggccgcgcc gccgagtacc gcgacaacga
    10741 gacgggcatg cacgtattgc gcatgagcca catcgcgcgc ctgattgcgt tgtcagtcgg
    10801 gctgggtgag gccgaatccg agctaatcct gcatgcttcg cccatgcacg atatcggaaa
    10861 gatcggcatt cccgatcgca tcttgctcaa gccagggccg ctcgacccgg aagagtggga
    10921 aatgatgaag cgccataccg tgatcggcgc cgagatcttg ggggatcacc cgtcggagct
    10981 gatccgcatg gcccgcctgg tcgcgctgcg acatcacgag cgctgggacg gcaaaggcta
    11041 tccggatggc ttgcgaggcg agcagatccc gatcgccgcg cgcgtcgtag ccatcgccga
    11101 cgttttcgac gcgctgctct cggtgcgccc atacaagcgc gcctggtccg tcgaagacac
    11161 cctggccaag ctgagcgcag agagcggtca ccatttcgat cccaccgtcg tcaacgcctt
    11221 cttgcgcatc ctgcccgagt gcttggaggt tcgggctcgt taccgcgacg attgagcgga
    11281 gcgcaccgcg cgccgcgact ttacttccgc ggcagcgccg ggttaacgtg gctcgcatca
    11341 accatgctgg atgtcgggat ccgcaaggcg ttcgcgtcgt ttctcgtgag tggagcgttg
    11401 gccctcggcg cgacgggctg cggacgagcg gcaggggcaa ctcccgcgga gctgggcaaa
    11461 gggcgcgatg cctcgggcac gggcgcgctg ctctacgaac gcaactgcca aagctgccat
    11521 ggcgaacaag gtcagggcac cggcaaagcc cccgctatat tgggccgacg cgcgctcgcg
    11581 tcggggcgct ttcgcaacgc agagaagcta ttcgattacg tcgcgcgcaa gatgccagcg
    11641 gacgaacccg gacgcctcga cattggccag tattggaaca tcgtcacgtt catggtcgcg
    11701 accggcggac accgcattcc cgacgggcgc ttgtccgagg ccaacgccgc caaaatcaag
    11761 ctgatcgacg acgacgattg aacctgtgaa acacaggtcg aagcgagagc ggcaataccg
    11821 cgatgactga agagtgatcg cgcgaagctc agcgcgtcga gaagtagacg atgacgagca
    11881 acagcaccac gagcacgatc gcgaacggcg cgaacgagct cgatcgcgat ttggattcgt
    11941 cgatcaagtg agcaaagctc ggctgcgccg ctctcgcccc accgccccgg gatccgccac
    12001 cacgcccgga cgtcgaggcg tcgtcgggat cggccttggc tttgccgttt cctttcccgc
    12061 gctcccgctt gctgctcggc ggcttttctc ctttgcccgc cgcctccgat ttttcggact
    12121 cgttgctgtc ctccttggag ctcgcgagcg acgcgcgtga tgatgcacca ttgcctcggc
    12181 ccgctccgtt cgcgagcggc aaagccaccg attcagccgc ggaggtttcg gccgggtgag
    12241 cttgatcgtc ccagttttcg acctgcgcca gcagccgcgc tcgttcggcg agcgactctt
    12301 gcgcgagccg cgcaatccag tccgcgactg gtccgggtga ggccacctcg accgcagcgc
    12361 ccagggcctc ggcgaaatca cgagccgttg ggtagcggtc ttgcgggcgg aacgccagcg
    12421 ctcgcatcac cacctcatcc accgccttcg gcagagaagg ccgatattcg ctcggtttct
    12481 ggtagttgcc gcgcgcgatc cgatccaaac gttgcttgtc cgagggcccg ggcgcgaagc
    12541 ggcgcagggt gagcaactcc cagagcacga tgcccgccga gaagatgtcc gacgagcgcg
    12601 tcaagcgctc gccccggatc tgctcggggg acatgtagcc ggtcttgcct ttgaggcggt
    12661 tcggaaccgt ctcttccatc tgctcgagcg ctttgacgat cccgaaatcg agcacgcgcg
    12721 cgatgccgtc cgtaccgatc aaaacattgg gcggcgagac gtcacgatgc acgagcccga
    12781 gcaactgccc cttctcgtcc gtagcctcgt ggactgaatg cagcccgtgt agcgcgccga
    12841 tcatcacgct cgcgacgatc ggcaccggga tccactcgcg cagcttggac tgggctctac
    12901 acagcgcgct caatgattcg ccgtgcacgt actcgagcac cagcaagact tcgtgcttga
    12961 gcgcgaccac gtccaacgtc ggcacgacgt tgggatgccg cacccgggcc gcgaggcgcg
    13021 cttctttgag cagcatctcg gcgaactcct gattcagaat caggtgcggc agtaggcgct
    13081 tgatggctac gacgcgcgaa aagccgcccg cgccgtccaa acggcccaag tgcacggtgg
    13141 ccatgccccc ggtcgcgaac ttatcgaaca gcgcgtaacg cccaatcttt gcgattggcg
    13201 cacgcacgga gatagggccc gccgcatgtg aaatgcgcgc ttggccgttg cgctcggccg
    13261 cggcgccttc cgcaacgtcg agtcgtgttc ctgcatccgg ggcggcattc cagccgctct
    13321 ccgattgcgg ctctacgatt ggagcacgcc cctcctcctc cgaatcaatc agtgaatccc
    13381 gggaacccgg cgcgggcgct acactcgcga agcccagccg agacatgggg ttgggcgggc
    13441 ctggggggcg gctcgagcgc gtgccatcag gacgaggagg cgagattgaa ccgctggcgc
    13501 gcggcggacg cgacgacgtc gaatgctcaa tggacgcacg tcgctcgatg ggcgccgccg
    13561 gaggcgggat attggagatc gacggtagag caggtgcagg tggaacgtcg ctctccaagc
    13621 cgctttggga aagcaaagat tcgggaggtg gcgcctggct aatctggcct tctggccgct
    13681 cctctaccgg tggcggcgga atagtgccgt ctactcggat tggcgcaccg cagctccggc
    13741 aagtgatcgt gacgacgcgg ccgcggacac gctgctcata gagcttcggc ggcacggaga
    13801 ggcgcttgcc gcacgattcg cagaggaaaa cgaaacccat taaatcagag gcccctcaaa
    13861 caagaatcaa agcgggcgac agcatagacc cacgtgaccc gggccatgca gccgtttcgc
    13921 agtcaggcca ctcccccgga agaaggaacc caaggagcac ccgacggatc gcatcgcgga
    13981 gattgtaagt gacagactca cctttacctc a
//
LOCUS       DASTKU010000081        23580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1129304, whole genome shotgun sequence.
ACCESSION   DASTKU010000081 DASTKU010000000
VERSION     DASTKU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 23580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23580
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129304"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..268)
                     /locus_tag="VFQ61_02740"
     CDS             complement(14..268)
                     /locus_tag="VFQ61_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HET9953390.1"
                     /translation="MIEPEVVKARLLEAFPGADIDLVDLTGTRDHYQARIVSERFQGK
                     TAIEQHQLVYQALGKEMDGPIHALALRTYTPESWAKTQSR"
     gene            complement(417..1442)
                     /locus_tag="VFQ61_02745"
     CDS             complement(417..1442)
                     /locus_tag="VFQ61_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953391.1"
                     /translation="MAFALAAAALLGATASSCGFWKGPVNASPSLRWWLFSNFGAQRM
                     CPEMLKRSTALRLTAGGNAVGRFFPEQCRTQVNDASQTVLVNFSGTGYAWTPVAGRVG
                     FWASAAVEYRMDFFLGDDAMYIWARTARIASGPEFRMGAIEYKLANFAAQGPAGYLLN
                     TFGSQLMESQLAKGFTVVHSDEGDAFWLGHLTPPQRPPSPFVQGKNRFVFANETTEVR
                     TDQVDLLGPFEVVDRDQALFLRMNVTGPAVDVLLVTRSAGDVFRAELQLGGVLKAPNT
                     VPVSTWVVRPGGEQQQMLRLPPGQYYAIVDNSARLGSVNPPWSPLGVIGGSTAVVSYI
                     AELGDAD"
     gene            complement(1535..2413)
                     /gene="hemH"
                     /locus_tag="VFQ61_02750"
     CDS             complement(1535..2413)
                     /gene="hemH"
                     /locus_tag="VFQ61_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET9953392.1"
                     /translation="MSSKDGVLLTAHGTVESLDDLPQFLSRIRRGRPAPAGLVEELRH
                     RYERIGGSPLLRITGEQAELLSASLGLPVYLGMRLWRPELSEALTRARQEGIERLCVL
                     PLAPFSVHVYVQAAEQALAQLGGPPPKLVGCPPFGRSPGFVQAWADAISAHERPEPGT
                     ELILTAHSLPTAIVRAGDPYQTEVEACARALGGALGRTYRLAYQSQGADGGDWLGPDL
                     PSTLRACRENGARRVLLAPIGFLSDHVETLYDLDVEARAQARELGLDFSRVPALNTSP
                     SLISALSDAARAAFLS"
     gene            complement(2416..4047)
                     /gene="pruA"
                     /locus_tag="VFQ61_02755"
     CDS             complement(2416..4047)
                     /gene="pruA"
                     /locus_tag="VFQ61_02755"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HET9953393.1"
                     /translation="MLPANEPVWSYAPGSPERAAIQRELQRQAESPVFVPMRIGGDAF
                     AGESSLALRAPHRHALLLGEAAQGGRAEMQRAIQAALTARAEWARRSQSARSAVFLRA
                     AELLAGRYRQTLNAATMLGQSKTVHQAEIDAACELCDFFRFNAHHAEELCEGRLGGDL
                     RSPAGQRNTWDYRPLEGFVYAVTPFNFTAIAGNLVSLPALLGNVVVWKPSPYAVLSAH
                     YLLQLLREAGLPEGVVQFVMGDAEALSEEALQHKQLSGLHFTGSTRVLKKLSQRVAEN
                     LPNYREFPRVVGETGGKGFIVAHKSAELDALCVATLRGGFEYQGQKCSAVTRVYVPRS
                     LAVGFERRLLELLASVKVGDPTDFSNFMAAVISRDAFDRLSLAIERARQDRTCRVAAG
                     GGCSDEAGFFVEPTLIQVLSPRHTLLSEELFGPVVAFYVYDDDRFEEVLGACDSGSDY
                     ALTGAIFARDPAAIETACSTLRYSAGNFYVNDKPTGAVVGQQPFGGSRQSGTNDKAGS
                     AQHLLRWVSPRVIKENFAPPTDYRYPYLLKDSGNE"
     gene            complement(4227..5279)
                     /locus_tag="VFQ61_02760"
     CDS             complement(4227..5279)
                     /locus_tag="VFQ61_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953394.1"
                     /translation="MLKNTLPHLALCLLLPLSACSLDKLDPSLDSSAAEYREALPDAK
                     AIALRGPESAGSSQRLQNSGQTPPESTGDQAAEDEAEPAGSGVAQWYAFTREIRDNVN
                     HVTREVLTLVSQIAQLEPTRFTANSAEWGPHTEALSPVTYRFMVVRVQEGEYKYRLEG
                     RPKTSTADADYLVLLEGKGYSEADERHGDGEFRFHPDNARTLDPSDPASHDGDAERWD
                     ALTVSYDLPRDPSTGAVPYTIYAHLSGSAPDIEVTSKATNVQGGSLVVVTQGDVDEAK
                     NTQLEDIVVESEWIATGAGRSDILISGGDLPKDPSSVQATECWGTDFARVYYTDSVSF
                     EPTEGDASACIERTSH"
     gene            5579..8929
                     /locus_tag="VFQ61_02765"
     CDS             5579..8929
                     /locus_tag="VFQ61_02765"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9953395.1"
                     /translation="MRLQKSPVDALLTLPHLAELDPCRANFRQAMAALGHRALRGVQG
                     APPLEGLNPEELQHAAERAIEIGLADDLDWLAPGPAALALYELSAALPPGKPRRELGR
                     RVYARIYQGTASTFAAVASRMALTSARPFEAAPLSARVRLLFSLSIGSAVNPGPLALT
                     LVTRDELRERWLETPRTGPLPARRLAAKLLEHAAREAVFRAQQGDPQPSEVLLDTPLR
                     AAHHALLSDREHLVWRHAAVARGLLSSIDTRCREEIELSLNPGLSPLEWRRGAVSLVA
                     LASVDPEHALRGLRRLLEGPQAELDKGLPATLTLGLPRVIEAEPDAAEQILEWISKRR
                     RPDVAEAIGALLTELPNASFAGKAMSKLRQALGPEPDSTNSFLRGLFERKWDRSSALD
                     EDAPSLLNRALLAYEHEGSRTAFEFATRTLQALGRSLDWLLLSASHEESQRLTQSLSV
                     LSDIDRTAFERACLHDLLLLGRRPGDTDVSVPELDRFHDRIGRFLLHAEAKTQRGASP
                     AQAFSEQRRLRTLLHLTDVDSTQKEDDKRVRQRISRSLEVLLDRLDEGDSFLAHRIVC
                     ATLARACDAAVRENLGEASDVLLLLASRVRSRESYRVIAEASTQPAVRAVLAAFTEFL
                     EFRETRDPISHTVFEHEGSFSDAMLRLSRGLLAGGSYRGEALRRVMLRLSRGLEQIQQ
                     ARGQGDLVAGTHVDNAEERVDVLGELEQTIDELLRLQRGALQRLLAQEPSAPNMPGEA
                     PSLSHVIERAVSTGTPADGSALHGAISALCAGLCEPIAFVISTTLSRIGLLPLEGGRE
                     VFSIPLERKREPLPDWLLPHRTIGAFYVVRPLGAGGTSSVFLARRLEQRSDPKAPGFA
                     LKTPDYDPTTARSLSEQEFLQLFRDEASALLALPEHPNLARFVTFDVGAQPKPILVME
                     LICGTRLDRIVRSGALTTERAFSYLDGILAGLEAMHRASVGHLDVKPSNVVLRDTDVP
                     VLVDFGLSGRHLRPGCGTLDYCSPEVLGVAPPGTRLSPLPTDLYAFACTAFEVLTSEA
                     LFHGEDEATVLSQHLSHDGWPTRLAPYAHTPETAELAKLLAACLRRDPEKRPTATQAR
                     AVLRRLAPNYTQAPWPLSLPEMVRRAAS"
     gene            8991..9500
                     /locus_tag="VFQ61_02770"
     CDS             8991..9500
                     /locus_tag="VFQ61_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953396.1"
                     /translation="MSIRLTKYVPFLCLAFASFTGCGGDEAPPPEVIPSLAFSSVLTS
                     VDTYDAMTEGPVAAEIGCDAVLAFAFTANDYSLTQPGNCESNPRCGHVRVSVSKEGLE
                     PLTVVGTGIAPLAVPLTDDQSVEAFTGSLHVEAVLLADDGSEFKTQAGDSIQTELDLE
                     IPVPSCPEP"
     gene            9946..10785
                     /locus_tag="VFQ61_02775"
     CDS             9946..10785
                     /locus_tag="VFQ61_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953397.1"
                     /translation="MGPLCALLFLVWLGEHPPPAGRFVASAGLSAVVLLFIMTTTGRT
                     FRIRVTPGSRLVWEEGLDAEDDYRVFVHTAEQRRLLLEHPDPARVLADLQRFQNEFGL
                     PLDGPSWLPKSPNPAERQPRALPGEEVGQYWPYQTRAAYAVLGSALFILAVFGFSVRA
                     EQELSPLSLVLPLATSATTALVGWFLLGLQMSVRVSAERLAFFTHAWGISWQSAEFAM
                     QDLRAVRAVGHAGFAPQHLLIETQHGSRALRLSEELLDRVLKRLEPLRNQGSEPMPQI
                     GAE"
     gene            10782..11507
                     /locus_tag="VFQ61_02780"
     CDS             10782..11507
                     /locus_tag="VFQ61_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953398.1"
                     /translation="MTTRSTASGDLRTSHRWLVPLAMLSFVWTASADPDRAPPHPAAS
                     GARPVPSAVRERLQQMMEKRGDRQPGDLRDRMEQMRERIREARERREHVRTATHDLDE
                     GLRKGDMKREDLDKRIDEYKKLRAERRLEHRAEIFRMWGLGLSQPEVKAELEMHARRM
                     AKLRRLEILVATDRKGEKRKKLADRIDQLREREKERHHTAMVKLLPARAAAASAVAAA
                     SVPNPALPAPSASPAPAASGGQP"
     gene            11504..11785
                     /locus_tag="VFQ61_02785"
     CDS             11504..11785
                     /locus_tag="VFQ61_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953399.1"
                     /translation="MTRAPRSYVALMALTILWSALGSTACRSKAPTPESKAAPSASAS
                     VARAPTPPPPPREPPNAPTEEDFEEEAESITAKNLEDELDKLEKELSAP"
     gene            11819..13267
                     /locus_tag="VFQ61_02790"
     CDS             11819..13267
                     /locus_tag="VFQ61_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET9953400.1"
                     /translation="MRSLSVAHHSLGRILGSLLLLLGVQCSKTDSNPSAGKPVSSAPL
                     GSTSGAVTRGAASSESLGQPGSPEGSARPPTDSAPSNPPPGTGEPLNVILLTIDSLRA
                     DMPWTGYSRPIAPFLTQLAERSAVYTRAYSASSYTAKSVATFLSGRYPSTLYRDGFFF
                     AKYSNANKFMAEILSERGVRTIGFHGHLYFGRGKGLEQGFAEWHLVPGITFDAQTDKH
                     VTSEKMTELGIQLLKNPANTAGQFFAWAHYMDPHDEYNKHAESPDFGRKGRDRYDSEV
                     WYTDHWVSKLIAYAEQQPWWKRTVLIVSADHGEAFGEHDMYKHAFELWEVLTHVPLLV
                     TGPGVRARRIDERRSHIDLAPTILDLMGVPVPPDMQGESLVPELRGATPTSREPILTE
                     LTEDSHNPPRRALIDGDYKLIDFGRSKFQLFDLKADPGELSDLSKQKPEELARMRKLL
                     EERYKKLPTVEPYGGAKLKEGGTARGPIGPSP"
     gene            complement(13414..13731)
                     /locus_tag="VFQ61_02795"
     CDS             complement(13414..13731)
                     /locus_tag="VFQ61_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953401.1"
                     /translation="MSIWGLGVALSVGFVEASRVAQISAPDSGSSTPDRLFQLARLEP
                     DPALPTGAQRCNTRCQSEQTDCAVRCDGEVSCIQGCQKVADSCVEKCSTPTPPPAAGA
                     SAP"
     gene            13998..17444
                     /locus_tag="VFQ61_02800"
     CDS             13998..17444
                     /locus_tag="VFQ61_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953402.1"
                     /translation="MFVSSRRRAEVVLIAALSGVLLAPGCELPEYAFPEAQLGQGGEG
                     ATAHASCTADEQCATLSTTRTCDLRTSECVQCVPTPDRDDCGPGQFCSVNRCAVGCDR
                     SADCKSGTTCDLASHVCVGCQGDQDCSAGTVCADGRCESSCATDETCPQDFTCCGGTC
                     AALGSDPLHCGACMQACADDEDCVNGECGAGSGCQKGSADCDGWRGNGCEVDLNRDDA
                     HCGVCGNDCGAAHCSGGRCSALQCGDGTADCDGNPNNACEAKLDATATCGSCSHQCSA
                     VHGTPSCLEGTCRIVCDIGYEDCDGNTETGCETKVADDARHCGACATRCENDHGSTLC
                     RNSACEPECAQGFQSCDGNPANGCETDIRSSALHCGSCDGACDPQHASGNCENGVCKV
                     VRCEDGYEDCDGDPRNGCEADLNSITSCGRCGNLCSENGGHASCQEGQCQIECDRDRR
                     DCSGGLLDGCETNTSLSVRHCGACGNACNPPSGNSAACNNGVCTFSNCSDPTADCVPD
                     DRAGGEGCETNVASDPKHCGGCGLECFFPNAEARCVAKSCVQGDCKPGFADCKHQQSD
                     GCETQLGTTNNCGACGHVCSPVHGSNSCVDGSCVPLCEPGWEDCDGIGENGCETPLDT
                     TTDCGKCRAACTFPNSAAATCEQGVCRITQCTPGFGDCDGRRETGCEARLNTENNCGT
                     CNVRCAPPHALADCSTGTCKQTGCLEGYADCRPEDAGCETLLGTSSNCEACGNACVTD
                     GGHVSSNACQGASGRWDCAPTCLPGWDSCEGNPDNGCEANLSSPEHCGGCGHVCDLPH
                     TALHACQAGSCKVAQCDAGYGDCNHDPEDGCELDVSADLANCGECQHACSTEHGQAMC
                     RDGACRIQCEVGFGDCNGRNEDGCELDTTSDPEHCGTCDVACSGATPFCTNRQCKDRL
                     EIAVVNRDTTALRTSGTTLLFKHNLQTPKLSGRPNYRAILVGVGARGNNNGARPQPPI
                     YGGRKMWLLKEVLSSNQAWAGIYALDDLGSASRDKLPDDPGQYDISISPNDAQNMFAL
                     MANVTELRNVELVTGLLDASAGSAGGACNGTTRGDDIALRPGSLIYSAFSVYQPSNIP
                     PTLTPTSSQTLNATPSWSNQTLFATALLNSSVTSASVRWSISACATTAQALVAIKPTS
                     SP"
     gene            17799..19649
                     /locus_tag="VFQ61_02805"
     CDS             17799..19649
                     /locus_tag="VFQ61_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953403.1"
                     /translation="MQIAQVLCLALAVTSCVVESAGDDDPKGGASSRGGASTGANSNT
                     GGNAGEGAGDGGTTSGGRTTDSSEGGTTLGRGGSLNPGGATGDESGGTTTRPPPLSQA
                     GTSSQADPCGDVPQEGRCIDQRSYEICAVPTGNAEPTVLERSCRAVERCVDGNTGARC
                     ELLPSSCVPGGSECTGGSNARQCSQEGTWMEATCPGGGSCFQTALGAFCEPTFKTTTY
                     EGNLTYEAFAPENDLSEWSAQPQSIPAENVLALSWHGEELIDATLIDEEGNYSLRVPS
                     ALDETDRITFFLMHPDSTGGSIEYAVFNPKLPNGEVEVPPSSLSGQFWQFAINPAEIP
                     SGTSVEIKEDDGSGAVHIYNYIGLARAFTQSMYGSAPGSVVAHFRPNTEFSCGACFLP
                     LAVDFGSMSFDSQLFFSATAQDRAYWADPVTVHEIGHYTMWAFGKLPGEGGPHCLGVP
                     ALPGIAWSEGWATGFSSIALDSPLYYDKQQGSMFWIDLERRRYSARRPWTRPDPQYGL
                     IQLMDENDVAAMLWQLAADERVGAELVLSSLTHPRITQSPFKRGYTRHVWEVDEQCLP
                     ADLSDTGESAPMFADFLDALRCAGAPAAAIDDVTEPETAYPYPSSSPICK"
     gene            19695..20291
                     /locus_tag="VFQ61_02810"
     CDS             19695..20291
                     /locus_tag="VFQ61_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953404.1"
                     /translation="MTRVLLGLHALLLLAACEKGAPKAATPTNGETNLSGVNSPANDA
                     AAAPADSVVDGSFKGRRAPLKVEVRGPDNPQPNSEIEVVVSIERRTPHLPVDLSVHLP
                     AGATLVRGLPSEHIETEGAKLERRYIVRLGSVPTTDIEITAQAYDQDLGVRGRGMYRF
                     GRPEPRLPEPNRSDRPTVLGDQDFGRPVELKPERNVRH"
     gene            complement(20430..21428)
                     /locus_tag="VFQ61_02815"
     CDS             complement(20430..21428)
                     /locus_tag="VFQ61_02815"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HET9953405.1"
                     /translation="MKSVVTHLGLLAVAGALAFSVWSREEKAERTKSETIEVWSGRAD
                     AVESVEFESKKRKVRVQPKSDAQGRYYVVRLDKEDEPPAPKPADSSTPPLPPPVKRES
                     QLFIGTKEAEQFIEKVAALKAVRAIGKVEAARNAEFGFDKPEGTIKVKVAGKEHVLTI
                     GGQTPGGTERYAKAAPSNEVFAIESDLVQSLNFADSRLLSHNLQGFEDEEVKRVRISK
                     AVKSRELVRMPEKKEAWADAATPTKADETALNWMTKLGRLRAINFVEKPTGNLEPIAM
                     VEYLAGTRPLGKLELYKTTGEKGPEYLARTAYTRWYVTVPTSAAEQVDQDLGALLK"
     gene            complement(21425..23521)
                     /locus_tag="VFQ61_02820"
     CDS             complement(21425..23521)
                     /locus_tag="VFQ61_02820"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HET9953406.1"
                     /translation="MAMTSNAPSPNAPGAPASANRGEPAPAWIFVLFVAGMLLVFLGQ
                     RVLLGFERLAPALSGLGLLAVLASTLLRFAPRFSTTGERRRIERQLAVLSGLGLLGLA
                     VYFATSDAGAAKLGLDKLTTDQRTRFEELARVVWISLIAISVVPMVFAETALLPMRRA
                     EHPESRRVRSATAAGLVLALAAVYGSLFVYFANGIDLKVDYSYFKTSQPSESTRKVAR
                     SLTGDPIRVVAFFPEVNEVRREVETYLKQAAAGASNLKVEVRDRLLVPKLAQELRAMQ
                     DGVIVLSRGTTTQTMQIGTDLEQARNKLKTLDRDFQEQLLKIAKSRRTAYVTVGHGEL
                     ADSSRSKPDSAERSGQIVRTLLQRQNYLVRDLGLGQGLGRDVPDDAEVVLILGPTTPF
                     LAEELASLRRYAERGGRLLLALEPEAIGEQELVRGEGSGLAAPPSPAPSAAVGGKAAA
                     SNGAVADSASAADAGFNGELAALAGLKFAPDLIANERQHLAVRHNDSDRTRLASNTFS
                     SHASVSTLSRNAPRSAIVVFGAGSLEKGGGSERVDFAVKSPTGSFQDGNHNFKLDAPA
                     EKSVSFNFAAAVTRALANPPAASDAKKDDKKNKPEPKDFKAMVFADADAFSDFVMGEV
                     IGNQILLVDAMRWLVGEESISQGLPNTEEDVRIDHTKQADSSWFYGTILGIPGLVLAS
                     GLLLSNRRSRGRGGKK"
BASE COUNT         4369 a         7605 c         7436 g         4170 t
ORIGIN      
        1 caatagagat gggttagcga ctctgcgtct tggcccatga ttcgggcgta tacgttcgca
       61 aggcgagcgc gtgaatgggg ccatccatct ccttgcccag cgcctggtac acgagctggt
      121 gttgctcgat ggcggtctta ccttgaaacc gttctgacac gatgcgggct tggtagtggt
      181 cgcgcgtccc cgtcaggtcg acgaggtcga tatctgcacc aggaaaagcc tccaaaagac
      241 gcgccttgac cacttccggc tcgatcatgg tcggagaaag ttacgcaccg aacccgagct
      301 gtcaactgcc cgttcttccg agtcgcgggg tgtctgcggc ccgccgctgc tctaaaccag
      361 ccttcattct ctcggcagcg cttccgcgtc gcagcgcgcc gtagaaacaa tcccgctcaa
      421 tccgcgtccc cgagctcggc gatgtaggaa accacggccg tgctgccgcc aatcacgccg
      481 agcgggctcc agggcggatt gacgctcccg agtcgagcgc tgttgtccac gattgcgtag
      541 tactgccccg gcggcaaacg cagcatctgc tgctgttcac cccccggcct caccacccag
      601 gtgctgacgg gaacggtgtt cggcgctttc aacacgccac cgagttgcag ctcagcgcgg
      661 aacacgtccc ccgcgctgcg ggtgacgagc agaacatcta cggccggccc agtcacgttc
      721 atgcgaagga acagagcctg atctcgatcc acgacctcga agggcccgag cagatcgacc
      781 tgatcggtgc gcacctccgt ggtctcgttc gcgaacacga agcggttctt gccttgtacg
      841 aaaggtgacg gcgggcgctg aggaggcgtg agatgcccga gccaaaacgc gtcgccctcg
      901 tcgctgtgca ctacggtgaa gcccttggcg agctggctct ccatgagctg ggacccaaac
      961 gtgttcagca ggtatcccgc cgggccctgt gccgcgaaat tcgcgagctt gtactcgatg
     1021 gcgcccatcc gaaactcggg gccgctcgcg atgcgcgcgg tgcgagccca gatgtacatc
     1081 gcatcatccc cgaggaaaaa atccatgcgg tactcgaccg cggccgaggc ccaaaaaccg
     1141 acccgccccg cgacgggcgt ccaagcgtaa ccggtgcccg agaagttcac gagcacggtt
     1201 tggctcgcat cgttgacttg cgtgcggcac tgttcgggga agaatctacc gacggcgtta
     1261 cctcccgcgg tcaaacgcag cgccgtgctg cgcttgagca tttcgggaca catgcgctgc
     1321 gcgccgaagt tcgagaacag ccaccaccgc aggctcgggc tcgcattgac ggggcctttc
     1381 caaaacccgc aactgctcgc ggtcgccccg agcaacgcgg cggccgcgag cgcgaacgcc
     1441 atgctacccc ggcggggtcg gagcttcgca gcacgtgcaa ggatcgaagg gcttcccatt
     1501 tcgactgaga tagtgggact ggctgagccg cagctcaact caaaaaggca gcccgagccg
     1561 cgtcggataa cgcagaaatc agactcgggc tcgtattgag cgccggcacc cgggaaaaat
     1621 cgagccccag ctcgcgcgcc tgcgcccgcg cctctacatc caagtcgtac agcgtctcga
     1681 cgtgatcgct caagaaccca atgggcgcca gcaacacccg gcgcgcgcca ttttctcgac
     1741 aggcgcgcag cgtgctcggc aggtcaggcc ccagccaatc tccgccatcg gcgccctgac
     1801 tttgataagc aagtcgataa gtgcgcccga gcgcgccacc cagggcacgt gcgcacgcct
     1861 cgacttcagt ttgataagga tcacccgcgc ggacaatggc cgtgggcaaa ctgtgcgccg
     1921 tcaaaatcag ctcggtgccc ggttcgggcc gttcgtgtgc gctgatcgcg tcggcccacg
     1981 cctgcacgaa cccagggctt cgaccgaacg gcgggcagcc aaccagcttc ggcggtgggc
     2041 cgccgagctg cgcgagcgcc tgctcggcgg cttgcacata gacgtgaaca ctgaagggtg
     2101 cgagcggcag gacacacagc cgctcgatac cctcttgtcg cgctcgtgtc agggcttcgc
     2161 tcagctccgg acgccatagg cgcatgccca agtaaacagg taggccaaga ctcgccgaca
     2221 gcagctccgc ttgttcgccc gtgatgcgca acaatgggga gccgccgatc cgctcgtagc
     2281 ggtggcgaag ctcttccacg aggcccgcag gggcgggccg gccacgccgg atgcgcgaca
     2341 ggaattgggg taagtcgtcc agactttcta cggtgccgtg agcggtcaac aacacgccgt
     2401 ctttggaact catgttcact cgtttcctga atccttcagc aagtacggat agcgatagtc
     2461 cgtcggcgga gcgaagtttt ccttgatcac gcgcggggac acccaacgca acaagtgctg
     2521 agccgaaccc gccttgtcat tggtcccgga ttggcgtgac ccgccaaacg gctgttggcc
     2581 cacgacggca cccgttggct tgtcgttgac gtaaaagttg ccagccgagt agcgcaacgt
     2641 cgagcacgcg gtctcgatcg cggccgggtc acgagcaaag attgctccgg tgagtgcata
     2701 gtcgctgccc gaatcacacg cgcccagcac ctcttcgaag cgatcgtcgt catacacgta
     2761 gaaagcaacc accggcccga acagctcctc ggagagcagc gtgtgcctcg gtgagagcac
     2821 ttgaatcaac gtgggctcga cgaagaagcc cgcttcatcc gaacacccgc cgcccgcggc
     2881 cactcgacag gtgcgatcct gccgggccct ctcgatggca aggctcaaac gatcgaacgc
     2941 atcgcgcgaa atcacggccg ccatgaagtt gctgaaatcg gttgggtcac cgactttgac
     3001 gctcgccaga agctccagca aacgtcgctc gaagcccacc gcgagcgagc ggggcacata
     3061 cacccgagta acggcggagc acttttgacc ctggtattcg aagccaccgc gcagggtcgc
     3121 gacgcacagc gcgtccagct ccgcactctt gtgcgccacg atgaagcctt tgccgccggt
     3181 ctcccccacc actcgcggga actctcgata gttcggcaag ttctccgcga cgcgctgcga
     3241 gagcttcttc aggacgcgcg tggaaccagt aaagtgcaag cccgaaagct gtttgtgctg
     3301 gagcgcctcc tcactgagcg cttccgcgtc ccccatcacg aactgcacta cgccttcggg
     3361 tagccccgcc tcacgcaata gctgcaacag gtagtgtgcc gaaagcacgg catagggtga
     3421 gggcttccaa accactacat tgccgagcag tgcgggcaag ctgaccaggt tgcccgcgat
     3481 ggcggtgaaa ttgaacggag tcaccgcgta cacgaagccc tcgagtggac gatagtccca
     3541 cgtattgcgt tgcccagccg ggcttcggag atcgcccccg agacgcccct cgcacaattc
     3601 ctcggcgtga tgggcgttga agcggaagaa gtcgcaaagc tcacaggccg catcgatctc
     3661 ggcttgatga acggtcttgc tctgacccag catggtcgcc gcgttcagcg tctgacggta
     3721 gcgaccggcg agcagctcgg ccgcgcgtag aaacacagcg ctccttgcgg actgggatcg
     3781 gcgcgcccac tccgcgcgtg cggtcagggc ggcttggatc gcgcgctgca tctcggctcg
     3841 cccgccttgc gccgcttcac cgagaaggag cgcgtgacgg tgcggagcgc gcaacgccaa
     3901 gctcgactct cccgcaaacg cgtccccccc aatgcgcatc ggaacgaaga cgggggattc
     3961 cgcctgacgc tgcagctctc gctgaatggc ggcgcgctcg gggctaccgg gcgcgtagct
     4021 ccacacgggt tcgttggcgg gcagcatgcc aacgcaaagc gtagtacgcg cccgcccaga
     4081 attacagccc ctcagaaggg ctttccataa gagcccgtcc aataaagcta ggccctcagg
     4141 aacggctcac tcgtgactga gctgctagag acgatgctcg ttggagccga agacgcttac
     4201 ccggggaggc tcgttggaaa ggtccgtcaa tgcgaggttc gttcgatgca ggcgctcgcg
     4261 tcgccttcgg tcggctcgaa gctgacggaa tccgtgtagt acacgcgagc aaagtctgtt
     4321 ccccaacact cggtggcttg aaccgagctc ggatccttgg gcaagtcgcc acccgaaatt
     4381 agaatgtcgg agcgcccggc tccggttgcg atccattcac tctcgaccac gatgtcttcg
     4441 agctgcgtgt tcttcgcttc atcgacatct ccttgtgtca ctactaccag gcttccgccc
     4501 tgcacattgg tagccttgga ggtgacctcg atatctggag cggaaccgct caggtgggcg
     4561 tagatagtgt acgggacggc tccggtgctg gggtcccgag gcaaatcata gctaactgtg
     4621 agcgcgtccc accgctcagc atccccgtca tgactggctg gatcgctggg atcgagcgta
     4681 cgcgcgttat ccgggtggaa gcgaaattct ccatcgccgt gccgctcgtc tgcctcggag
     4741 tagcccttgc cctcgagcaa caccaagtag tctgcgtcgg cggtcgaagt cttgggacgc
     4801 ccctccaggc ggtacttgta ctcgccctct tgcacgcgca cgaccatgaa ccgataggtc
     4861 acgggagaga gcgcctctgt atgcgggccc cactccgctg agttggcggt aaagcgggtg
     4921 ggttcgagct gggcaatctg agaaaccaac gttaggactt cccgggtcac gtggtttacg
     4981 ttgtcgcgaa tctcgcgggt gaaagcgtac cattgcgcga ccccacttcc ggcgggctcc
     5041 gcttcgtctt ccgcagcctg gtcccctgtg gattccgggg gagtttgccc tgaattctgc
     5101 agtcgttggc tagaacctgc ggactctggc ccgcgcaggg cgatcgcttt ggcgtctggc
     5161 aacgcttccc ggtactccgc cgcggaggaa tcgagcgacg ggtccaactt gtccagggaa
     5221 caagccgaga gcggtaaaag caggcagagc gcgaggtggg gcaaggtgtt cttcagcatt
     5281 tggggagccg atccttcttg gctaaaacgc acacgcatgt gcatgcggcg cgggcgacct
     5341 caggggtcca tcaaggagca cgccagcctg gaaaacctcg aaaagaacgg gatcgctggg
     5401 gagactgtga acggcgctgc tcacgacagt gaacttcgcg ggcggcagcg caggcctgag
     5461 ccggctcccc tcacccggct acaccgcgag tttctgggca aactgaggct tctgagcaaa
     5521 gtttctgagc aaaatggttg ggaatgtacc tgaacacccc tagactgaag ggtattcaat
     5581 gcgtctccaa aagagccctg tcgacgcgct gctgacgctg ccgcacctcg cagagttgga
     5641 tccttgtcga gcgaactttc ggcaggccat ggcggcgctc ggacatcgcg cgttgcgagg
     5701 tgtgcaaggc gcaccgccgc tcgagggctt gaacccggaa gaactacagc acgccgccga
     5761 gcgggcgatc gagattgggc tcgcggatga tctcgactgg ctggcgcccg ggcccgccgc
     5821 gctcgcgctc tacgaactca gcgcggccct gccgcctggc aagccgcggc gtgagctcgg
     5881 ccgccgcgtg tacgcgcgaa tctatcaagg caccgcgagc accttcgcgg ccgtcgcgtc
     5941 gcggatggcc ctgacttcgg cgcgtccctt cgaggccgcg ccgctcagtg cccgagtgcg
     6001 cttgctgttc agtctctcca tcggatcggc ggtgaatccc ggccctctcg ccttgacact
     6061 cgtcacccga gacgagctac gcgagcgttg gctggagacg ccgcgcaccg gtcccctccc
     6121 ggcacgccgg ttggccgcca agctgttgga gcatgccgcc cgtgaagccg tgttccgagc
     6181 ccagcagggg gaccctcaac cgtccgaggt gctgctcgat acaccacttc gcgcggcaca
     6241 ccatgctctg ctttccgacc gtgaacacct ggtctggcgg cacgcggcgg tggcgcgcgg
     6301 gttgctctcg tcgatcgata ctcgatgtcg cgaagagatt gagctttcgc tgaatccagg
     6361 gctgtcaccg ctcgaatggc ggcgcggcgc ggtttccttg gtggcgctgg cctccgtgga
     6421 ccccgagcac gccctacgcg ggctgcggcg gctactcgaa gggccacaag ccgagctgga
     6481 caagggctta cccgccacgt tgacgctcgg gttgccacga gtaatcgagg ccgaacccga
     6541 cgccgctgaa cagatcctcg agtggatctc gaaacgccgc cggccggacg ttgcagaagc
     6601 catcggcgct ctgttgaccg agctgccgaa cgcgagcttc gccggcaagg cgatgtcgaa
     6661 gctacgtcag gctttgggtc ccgaaccgga tagtacgaac tcctttttgc ggggtctgtt
     6721 cgagcgcaag tgggaccgca gcagcgcgct cgacgaagac gcgccgagcc tactgaaccg
     6781 ggcgctcctg gcctacgaac acgaaggctc gcgcacggcg ttcgagtttg cgacgcgcac
     6841 gctgcaggct ctcgggcgtt cgctggattg gctattgttg agcgcatcgc atgaagagag
     6901 ccagcgcttg acgcagtcgc tctccgtact gagtgacatc gatcgcacgg ccttcgaacg
     6961 ggcgtgcttg cacgatctgc tgctgctcgg gcgccgcccc ggggacacgg acgtgagcgt
     7021 gcccgagctc gatcgcttcc atgaccgcat cggccgcttc ctcttgcacg ccgaagcgaa
     7081 gacccagcgc ggtgcttctc ccgcgcaagc cttctcggag cagcgccggc tgcgcactct
     7141 gctgcacctg acggacgtcg actccactca aaaagaggat gacaagcgcg tacgtcaacg
     7201 catcagccgc agcctagagg tgctgctgga tcgactggac gaaggcgatt cgttcttggc
     7261 tcaccgcatc gtatgcgcga cgctggcgcg cgcctgcgac gcggcggttc gcgagaacct
     7321 gggtgaggcc tctgacgtac tgctcctgct cgcgagccgc gtgcgtagtc gcgagagcta
     7381 tcgtgtgatc gccgaagctt ccactcagcc cgccgtgaga gcggtgctcg ccgcgttcac
     7441 ggagtttctg gagttccgcg aaacccgcga ccctatttca cataccgtgt tcgagcacga
     7501 gggcagtttc tcggacgcta tgctgcgttt gagccgcggc ctgctcgccg gtgggtcata
     7561 ccgcggagag gcgctccggc gggtgatgtt gcggctcagc cgaggcttgg agcagatcca
     7621 gcaggcgcgt ggccagggcg atctcgtggc gggaacgcac gtcgacaacg cggaggagcg
     7681 agtggacgtg ctgggcgagc tcgagcagac catcgacgag ctgctgcggc tgcagcgtgg
     7741 cgccctgcag cgcttgctcg cgcaagagcc gagcgcaccg aacatgcccg gcgaagctcc
     7801 gagtctctcc cacgtgatcg aacgcgcggt gagtacgggc acgcccgcgg acggcagcgc
     7861 gctccatggg gccatctccg ccctgtgcgc cgggctttgc gagccgatcg cgttcgtgat
     7921 ctcgaccacc ctctcgcgaa tcggcctgtt gccgctcgag ggcggtcggg aagtgttctc
     7981 catcccgctc gaacgaaaac gcgagccgct gccggattgg ctactgcccc accgcaccat
     8041 cggcgccttc tacgtcgtcc ggcctttggg ggcaggtggc acttcttccg ttttcctcgc
     8101 gcggcggctc gagcaacgca gcgaccccaa ggccccgggg ttcgcgctga agacacccga
     8161 ttacgatccg acgaccgcgc ggagcttgtc cgaacaagag ttcttgcagc tgttccgcga
     8221 cgaagcgtcc gccttgttgg ctctcccgga gcacccgaat ctcgcccgtt tcgtgacctt
     8281 cgatgtcggc gcgcagccga agccgatcct cgtgatggag ctgatttgcg gcactcgact
     8341 cgatcgcatc gtgaggtctg gcgcattgac caccgagcgc gccttctcct acttggatgg
     8401 aatcctggcg ggcctcgagg caatgcatcg ggcaagcgtg gggcacctgg acgtcaagcc
     8461 ctccaatgtc gtgctgcgcg acacggatgt gcccgtgctc gtggactttg gcttgagcgg
     8521 acgacacttg cgcccgggct gcggcacgct ggattactgt tcgccggagg tgctcggggt
     8581 ggcgccgccc ggcacgcgct tgtcgccact tccgaccgat ctctacgctt tcgcgtgtac
     8641 cgccttcgag gtgctgacct cggaggctct gttccatggt gaagacgaag ccacggtgct
     8701 gagccagcac ttgtcgcacg atggctggcc cacacgtctg gcaccctatg ctcacacgcc
     8761 cgagaccgcg gagctggcca agctgttggc agcgtgtttg cgtcgcgatc cggagaagcg
     8821 cccgaccgca acccaggcgc gagccgtact gcgccgcctc gcgcccaatt atacccaagc
     8881 gccgtggccg ctatctttgc cggaaatggt acgccgcgcc gcgagctgac gcgcacgggg
     8941 caccaccacg agtactgtgt ctcgacgcaa ttggtgctac gacccggccc gtgtcgatcc
     9001 gattgactaa gtacgttccc tttctgtgtc tggcgttcgc ttccttcacg ggctgtggcg
     9061 gcgacgaggc gccaccaccc gaggtcatcc cgagcctggc tttctcgagc gtgctcacga
     9121 gtgtcgacac gtacgatgca atgaccgagg gacccgtggc ggccgagatt ggctgcgatg
     9181 ccgtgcttgc gttcgccttc accgcgaacg actactcttt gacccaaccg gggaactgcg
     9241 agtccaaccc gcgctgcggc cacgttcgag tcagcgtcag caaagagggc ctggaacctc
     9301 tgacggtggt gggcaccgga atcgcgccgc tcgcggtgcc attgacagat gatcaatctg
     9361 tcgaggcgtt tacgggaagc ctgcacgtcg aggcggttct attggcggac gacggcagcg
     9421 aattcaaaac gcaagccgga gactccatcc aaacggaact ggatctagag attccggtcc
     9481 ccagctgtcc cgagccgtaa ccgcagcatg gaaaaaaaag agccccgcga agcgagtgag
     9541 ggggagtcgc ttcgcggggc cggaaaaagc gggcagcagc ctgggcggag gggggggaca
     9601 cctcaagctg ccgccgtcga gacttaactg cacaaatcgg accagcactc gacttacgtc
     9661 atatcgataa cttgcaatgc gataatcgac ctatgcattt cagatctgca atgcagattt
     9721 gctaggcgca aaccgacatg aagccgctgg aggcccacgc cagcctcccc gactcacttc
     9781 ttcgctcgga aggcacacct tcccaagcat caaaaatgag acgggagctt ggcggcctat
     9841 ccggagctga tgtacggacc cggaagcttg cgaacgctac gggacgtccg ttcagggacc
     9901 gcgtgggtcc atcgtcgctc cgcggggcgg gctctggtgc ttgccatggg cccactctgc
     9961 gcgctcttgt tcttggtttg gctaggagaa catccgccgc cggccggacg cttcgttgcc
    10021 agtgcggggc tcagtgcggt cgtgctcctg ttcatcatga ccaccaccgg tcgcaccttt
    10081 cggattcggg tgacgccagg gtcgcgcctg gtctgggagg aagggctcga cgccgaggat
    10141 gactatcgcg tgttcgtgca cactgcagag caacgccgcc tcctcctgga gcacccggat
    10201 ccggcgcgcg tgctcgccga tctccaacga tttcagaacg agttcggctt gcccctggat
    10261 ggcccgagct ggctccccaa atcgccaaac cccgcggaac gacagcctag ggctttgcct
    10321 ggggaagaag tgggccagta ctggccgtat cagacgcggg ccgcttacgc ggtcttgggc
    10381 tccgcgctgt ttatattggc ggtgttcggt ttttcggtgc gcgccgaaca ggagctctca
    10441 ccgctgagtc tggtcctacc tctcgcgacg agcgcgacga cggctttggt cggatggttc
    10501 ttgttgggct tgcaaatgag cgttcgcgtc agcgctgagc gcctggcctt ctttacgcat
    10561 gcttggggaa tcagctggca aagtgcggag tttgcgatgc aagatctgcg agccgtgcga
    10621 gccgtggggc atgcgggctt cgcgcctcag cacttgttaa tcgagacgca gcacggatcc
    10681 cgagcgctgc gtctatccga agagctcttg gatcgtgtcc tgaagcgcct cgaaccttta
    10741 cgcaaccagg gctctgagcc catgccgcag attggagccg aatgactacg agatcgactg
    10801 cctctggaga tctacgcacg agccatcgtt ggctcgtgcc gctggcaatg ctgagcttcg
    10861 tatggactgc atcggcagat cccgatcggg ctcctcctca cccggcggcg agcggcgctc
    10921 gacccgtacc gagcgccgtg cgtgaacggc tgcaacagat gatggagaag cgcggcgatc
    10981 gtcagcccgg ggacctgcgt gatcgcatgg agcagatgcg tgagcggatc cgggaagctc
    11041 gcgagcgccg ggagcatgtc cgcacggcta ctcatgattt ggacgagggc ttgcgcaaag
    11101 gtgacatgaa gcgtgaggac ctcgacaagc gcatcgacga gtacaagaag ctgcgcgcgg
    11161 aacgccgact cgagcatcga gcagagatct tccggatgtg ggggcttggg ctaagccaac
    11221 cggaagtaaa ggccgagctc gaaatgcacg cgcgccgcat ggccaagcta cggcgactcg
    11281 agatcttggt cgcgaccgat cgcaaaggtg agaagaggaa gaagctcgcc gaccgcatcg
    11341 accaactgag agagcgtgaa aaggagcgcc atcacacggc tatggtcaag ctcttgcccg
    11401 cccgtgccgc cgccgcttcg gccgtagccg ctgcgtcggt tccaaacccg gcgcttcctg
    11461 cgcccagcgc gagccctgcc ccggccgcaa gcggaggtca gccatgactc gtgcgccgcg
    11521 ttcatacgtc gcgttgatgg ctttgactat cttgtggtcg gcactcggga gtacagcgtg
    11581 ccgcagcaag gcccccactc ctgaaagtaa ggccgctccg tccgcgagcg cctcggtggc
    11641 acgcgctccc acgcccccac ctcccccgcg cgaacccccc aatgcgccca cagaagagga
    11701 ttttgaggag gaggccgaaa gcatcacggc caagaacttg gaagatgagc tcgacaaatt
    11761 ggagaaagag ctctccgcgc cttgatggcg tcgttctaag cctgaaatcc tctgaatcat
    11821 gagatcgctc agcgtcgcgc atcactctct tggtagaatt ctgggcagcc tgttgttgct
    11881 gctcggggtt caatgcagca agaccgactc gaatccttct gcaggtaagc cggtcagctc
    11941 tgcgccgctt ggctccacga gcggcgcggt gacgcgcggc gccgcctctt ccgagagcct
    12001 gggtcagccg gggtctcccg agggaagcgc tcgaccgccg acagatagcg ccccatccaa
    12061 tccgccgccg ggcaccggtg agcccttgaa cgtgatccta ctcacgatcg acagcctgcg
    12121 cgcggacatg ccttggaccg gctactctcg gccaattgcg ccatttctca cgcagctcgc
    12181 cgagcgatcg gcggtgtaca cgcgcgcgta tagcgcctcg agctacaccg ccaagagcgt
    12241 ggccacgttc ctgagtggcc gctatccgtc gactctgtac cgggacggat tcttctttgc
    12301 gaagtactcg aacgccaaca agttcatggc ggagatcctg agcgaacgcg gggttcgcac
    12361 gattggtttc cacgggcacc tctacttcgg gcgcggaaag ggtctggagc agggcttcgc
    12421 agagtggcac ctcgtgccgg gcattacgtt cgacgcccag acggacaagc acgttacctc
    12481 cgagaagatg accgaactcg ggatccagct cctgaagaac cccgccaaca cggcaggcca
    12541 gttcttcgcg tgggcccact acatggaccc ccacgacgag tacaacaagc acgctgagtc
    12601 cccggacttc ggtcgcaagg gcagagatcg ctatgactcg gaagtctggt acaccgatca
    12661 ctgggtctcg aagttgatcg catacgccga acagcagccg tggtggaagc gaacggtgct
    12721 gatcgtgagt gcagatcacg gcgaggcgtt cggtgaacac gacatgtaca agcatgcctt
    12781 cgagctctgg gaggtgctga cgcacgtgcc cctgctggtg acgggcccgg gcgtccgcgc
    12841 gcgtcgcatc gacgagcgcc gttctcatat cgacttggcg cccaccatcc tggacctgat
    12901 gggcgtgccg gtgccgcccg acatgcaagg ggaatctctc gtacctgagc tgcgcggtgc
    12961 gacaccgaca tcccgcgaac ccatcctgac cgagctcacg gaagattctc acaacccgcc
    13021 gcgaagagcc ctgatcgacg gcgactacaa gctcatcgac ttcgggcgct cgaagttcca
    13081 gctgttcgat ctcaaagctg atcccggcga gctcagtgat ctctccaagc agaagccaga
    13141 agagctggcg cgcatgcgaa agcttctcga agagcggtac aagaaattac ccacggtgga
    13201 gccctacggg ggcgccaagc taaaagaagg cggcaccgct cgggggccga tcggtccgag
    13261 cccctgagaa ggtccgcgat ctcgagtcca ggcgatttct ccgaggtgtc gagccggcct
    13321 gaggtggcgt tcagctcgtc agtcgctcgc cactatgcag cgactgccta gtctagcgac
    13381 tgccaatgta tagcgactgc caatgtatgg cgactaaggt gcgctcgcac cggctgccgg
    13441 cggaggagtc ggcgtggagc acttctcgac gcaagagtcc gcaacctttt ggcagccttg
    13501 gatgcagctc acctctccgt cgcagcgcac ggcgcaatcc gtctgctcgc tctggcaacg
    13561 cgtgttgcaa cgctgggcgc ccgttggcaa agccggatcg ggctccaaac gagccaattg
    13621 gaacaaacgg tcgggcgtcg aagagccgga gtcgggcgcg gagatctgcg cgacgcggct
    13681 cgcttccacg aagccgaccg acagggcgac gccgagcccc cagatgctca actggagcgc
    13741 gtagcgcgaa gcgcgctcga gccaccccga cgccgaactc gtcgcggcga aaacttgaac
    13801 ccgcgctacc cgagcccgcg atgggctccc gactcgagaa gcatttgaac taacctgttg
    13861 agaggacctg agtcgcacgc gccgccgccg cataccactg atgaacggcg gattgcgcgc
    13921 aactttcagc ccaaaactgg agtttggact cgcgattggc caagtaggac ccctggagtt
    13981 gttgtacgct gcgcgccatg ttcgtgagct cccggcggcg cgccgaggtc gtgctgatcg
    14041 ctgcactctc gggtgtgttg ttggcgcccg gctgcgagtt gcccgagtat gcatttcccg
    14101 aagcgcaact cgggcaaggt ggcgaaggcg cgacggcaca cgcgagctgc accgcggatg
    14161 aacagtgcgc gacgttgtct accacccgga cctgtgacct tcgcacgtcg gagtgcgtcc
    14221 aatgtgtgcc cactcccgat cgagacgact gcggccccgg ccagttttgc agtgtgaatc
    14281 gctgtgcggt cggctgtgac cgcagcgcag actgtaaatc aggtacgacc tgcgacctgg
    14341 cgagccacgt gtgcgtcggc tgccaagggg accaggattg ctcggccggc accgtgtgcg
    14401 ccgatgggcg ctgtgaatcg agctgcgcta ccgatgagac ttgcccacag gactttacgt
    14461 gttgcggggg tacctgcgcg gcgctgggca gtgatccatt gcactgcgga gcttgtatgc
    14521 aggcttgcgc ggatgacgaa gactgcgtga acggtgagtg cggcgctggc tctggttgtc
    14581 agaagggctc cgcggactgc gacggctggc gtggcaatgg ctgtgaagtc gacctgaatc
    14641 gagatgacgc tcactgcggg gtgtgcggca acgactgcgg agctgcacat tgtagcggcg
    14701 gccgctgctc ggctctgcaa tgtggcgatg gcacggcgga ttgcgacggc aacccgaaca
    14761 acgcctgcga agccaagctc gacgcgaccg cgacgtgcgg cagctgcagt catcagtgca
    14821 gcgcggtgca cggcaccccg agctgtctcg agggcacgtg ccgaatcgtg tgtgacattg
    14881 ggtacgagga ctgcgacggc aataccgaga ctggctgcga gaccaaagtg gcagacgacg
    14941 cgcgccattg cggggcgtgc gccacccgtt gtgagaacga ccacggctcg acgctatgca
    15001 gaaacagcgc gtgcgagccg gagtgtgcgc aaggcttcca aagctgcgac ggtaaccccg
    15061 ccaacggctg cgagacggac attcgtagct ccgcccttca ctgcgggagc tgtgacggcg
    15121 cgtgcgatcc gcagcacgcc agcggtaact gcgaaaacgg cgtgtgtaag gtcgtgcgct
    15181 gcgaagatgg ctacgaagac tgcgacggtg acccacgcaa tggctgtgaa gcggacctga
    15241 acagcatcac gagctgcggt cgctgcggca acttgtgcag cgagaatggt ggccacgcca
    15301 gctgccaaga ggggcaatgc cagatcgaat gcgatcgcga ccgccgcgac tgctccggtg
    15361 gcctgctgga cggctgcgag acgaatacga gcttgagcgt tcgccactgc ggcgcatgtg
    15421 gcaacgcttg caaccctccg agcggtaatt ccgccgcttg caacaacggc gtctgcacct
    15481 tctccaactg ctccgatccc accgcagatt gcgtgcccga cgatcgagcg ggtggtgaag
    15541 gttgcgagac gaacgtcgct tcggatccga agcactgtgg aggatgcggc ctcgagtgtt
    15601 tcttcccgaa cgccgaggcg cgctgcgtcg ccaaatcgtg cgtgcaaggc gactgcaaac
    15661 ccggcttcgc agactgcaag caccagcaat cggatggctg cgagacgcag ctcggaacta
    15721 cgaacaactg tggtgcttgc ggtcacgtgt gcagccccgt gcatggtagc aacagctgcg
    15781 tcgacggcag ctgcgtaccg ctctgtgagc cgggctggga ggattgcgac ggaatcggcg
    15841 agaacggctg cgaaacgccc ctcgatacga ccaccgactg cggaaagtgc cgcgcggcgt
    15901 gcacgttccc gaactccgcc gcggcgacct gcgagcaagg cgtgtgtcgc atcacccagt
    15961 gcacgcccgg ttttggggat tgcgatgggc ggcgggaaac aggctgcgaa gctcgcctga
    16021 acacggaaaa caactgcggc acttgcaacg ttcgctgcgc tccccctcat gcgctcgcgg
    16081 actgcagcac cggcacctgc aagcaaaccg gttgcctcga aggctacgcc gattgtcgcc
    16141 cggaagacgc gggctgtgag accctgctcg gcacgagcag caactgtgaa gcatgcggca
    16201 acgcttgcgt gacggatgga gggcacgttt cttcgaatgc ctgccaaggg gcctcggggc
    16261 gttgggactg cgcgccgacg tgcttgcccg gctgggactc ctgtgaaggc aacccggaca
    16321 atggctgtga ggccaatctt tcttcacccg agcactgcgg tggctgcggc cacgtttgcg
    16381 atctgccgca caccgcgctg cacgcatgtc aggctggatc atgcaaggtc gcgcaatgcg
    16441 acgccggata cggcgactgc aaccacgacc cggaagacgg ctgtgagcta gacgtctccg
    16501 ccgacctcgc caactgtgga gaatgccaac acgcctgcag caccgagcac ggccaggcga
    16561 tgtgtcggga cggagcctgc cgcatccaat gcgaggtggg cttcggggac tgcaacggcc
    16621 gcaacgaaga tggctgcgaa ctcgacacca cgagtgatcc ggagcactgc gggacatgcg
    16681 acgtagcctg ttcgggggca actccctttt gcaccaatcg gcagtgcaaa gaccgactcg
    16741 aaattgcggt cgtcaatcgg gacacgaccg cgctgcgcac gtcgggcacc accctcctct
    16801 tcaaacacaa cctgcagacg ccgaagctct cgggccgacc caactaccgg gccatattgg
    16861 tcggcgtggg cgcgcgcggc aacaacaacg gggcgcggcc gcaaccgcca atctacggtg
    16921 ggcgcaagat gtggttgctg aaggaggtcc tctcctccaa ccaagcctgg gcgggcattt
    16981 atgcgctcga tgacctcgga agcgccagcc gcgacaagct gcccgatgac cccggacaat
    17041 acgatatttc gatctccccg aatgacgcgc aaaacatgtt cgcgttgatg gccaacgtga
    17101 ccgagctaag gaacgtggaa ctagtcacag ggctgctcga cgccagcgcc ggtagcgcag
    17161 gcggagcgtg caacgggacc acgcgcggag acgacatcgc gctgcgcccc ggtagcttga
    17221 tttactcggc cttcagcgtg taccaaccga gcaatatccc cccgacccta acgcccactt
    17281 cgagtcagac cctgaacgca acgccgagct ggtcgaatca aaccctattc gcaacggcgc
    17341 tgctgaactc cagcgtaacc agcgcgagcg tgcgttggtc gatcagcgcg tgcgccacca
    17401 cggcccaggc gctggtcgcg atcaagccga cctcgagccc atgaaggacg ggtcaagctc
    17461 gcaaccctcg taagttgcag gctcgcaacg tcgtgccgtt ctgcaggggc gcgtgtgcgc
    17521 cgctcgccct gcgggtggag atgctgcgca cttcccaccg cgagcggggc ttcgctcagc
    17581 tgattttctc ggagttcgac cgcagcgcgg ctgaccgcgg gctcgccgtt ggcagcacac
    17641 tgtacgctga tctaccaggg tcagaatcgt tgcctcgact tgcagagcca ccacggatac
    17701 cgtagcctct cggcacgatg gtttgggtgg aacgccccgc gttcaagtct tcggtcggaa
    17761 caactcacac tcaatcacgg caggcgcact cgccgcggat gcaaatagcg caagtgctct
    17821 gccttgcgct cgcggtaacg agctgcgtcg tggagtctgc cggtgatgat gatcccaagg
    17881 ggggcgcttc cagtcgaggc ggcgcgtcca cgggtgccaa ctcgaacacc ggcggcaacg
    17941 cgggcgaagg agctggagac ggcggcacta cgagcggtgg ccgaacgacg gatagttcgg
    18001 aaggcggcac cacgctgggt cgtggcggtt ctctgaatcc cggaggtgct accggtgatg
    18061 agagcggcgg tacgacgacc cggccgccgc cgctctcgca ggcggggact tcgagtcaag
    18121 ctgacccttg tggtgacgtg ccgcaggaag gccgctgcat cgatcagcga agctacgaga
    18181 tctgcgccgt gcccaccggc aacgccgagc cgacggtact cgagcgcagc tgccgtgcgg
    18241 tggagcgctg tgtggatggc aacaccggcg cacgctgcga gctgctcccg agttcgtgcg
    18301 ttcctggcgg aagcgagtgt accggcggga gtaacgcccg acagtgcagt caggaaggca
    18361 cctggatgga ggccacctgc cctggtggag gaagttgctt ccagacggcg ctcggtgcgt
    18421 tctgcgagcc gacgttcaag actacgacct acgaaggcaa cctcacgtac gaagcattcg
    18481 cgcccgaaaa cgacctgtcc gagtggagcg cgcagcctca gtcgattccg gccgagaatg
    18541 tgcttgcgct gagctggcac ggcgaggaac tgatcgacgc caccctgatc gacgaggaag
    18601 gaaactactc gctgcgggtg ccgagcgcgc tggacgaaac cgatcgcatc acgttcttcc
    18661 tcatgcaccc ggacagcacg ggcggctcga tcgagtacgc cgtgttcaac ccgaagctgc
    18721 cgaacggcga agtggaagtt ccgccctcct cgttgtcagg gcaattttgg caattcgcca
    18781 tcaatcccgc cgagattccc tccggtacgt cagtcgaaat caaggaggac gacggtagcg
    18841 gtgccgttca catctacaac tatatcgggc tcgcccgcgc gtttacgcaa tccatgtatg
    18901 gctccgcgcc gggctcggtc gtggcgcact tccgcccgaa caccgagttc agctgtggag
    18961 cgtgcttctt gccactcgcc gtggatttcg ggtccatgtc gttcgactcg cagctgttct
    19021 tctcggccac cgcgcaagac cgggcgtact gggcggatcc ggtgacggtg cacgagatcg
    19081 ggcactacac gatgtgggcc ttcggcaagc tgccaggtga aggcggcccg cattgcctcg
    19141 gagtgcccgc gctacccggg attgcctgga gcgaagggtg ggccaccggt ttctcgagta
    19201 tcgcgctcga ttcgccgctc tattacgata agcagcaggg ctcgatgttc tggatcgatc
    19261 tggagagacg tcgctattcc gcgcgtcgcc cctggacacg cccagaccct caatacgggc
    19321 tcatccaact aatggacgaa aacgacgtgg ccgcaatgtt gtggcagctc gccgcagatg
    19381 agcgagttgg cgcggagctc gtgctgagtt cattgaccca ccctcgaatc acgcaatcgc
    19441 ccttcaaacg cggctacacg cgccatgtct gggaggtcga cgagcagtgt ttgccggcag
    19501 atctctccga tacgggcgag tcagctccga tgttcgcaga ttttctggac gccctgcgtt
    19561 gtgcgggagc tccggccgcc gcgatcgatg acgtcaccga gccggagacg gcgtatcctt
    19621 atccaagcag ttcgcccatt tgtaagtaag gccaaccagg atccgcggtg cgcacaaagg
    19681 ccaatgaggt tttcatgaca cgagttttgc ttggtttgca cgcgctcctt ctgctggcag
    19741 cctgtgagaa aggcgctccc aaggccgcaa cccccacgaa tggcgagacc aacctctcgg
    19801 gcgtgaactc ccctgcaaac gatgcagcgg ccgctcccgc ggactccgtg gtcgacggca
    19861 gcttcaaggg gcgccgcgct cccctgaaag tcgaggtgcg cggacccgat aatccgcagc
    19921 ccaactccga gatcgaagtg gtcgtgtcta tcgagcgccg cacaccgcac ttgccagtcg
    19981 atctcagcgt gcatttgccg gcaggggcaa cgctcgtgcg tggtttgccg tccgaacaca
    20041 tcgagaccga aggggctaag ctcgagcgcc ggtacatcgt gcggctcgga agtgttccca
    20101 ccacggacat cgagatcacg gcacaggcgt acgaccaaga tctgggcgtg cgcgggcgcg
    20161 ggatgtaccg tttcggacgc ccagagcccc ggttacccga gcctaaccgc tcggatcggc
    20221 ctaccgtact cggggatcag gactttggac gtcccgtcga gctcaagccc gagcgcaacg
    20281 tccggcactg agcaacgtcc ggcactgagc aacgtccaca tcggagcggt gcggcggctc
    20341 gcatgaggcg caaggtggag gcacctgagt tccaccttgc accccgcttg cgcgctgcag
    20401 ccgtgctcag gtcacttgac gtgctcaggt cacttgagaa gagcgcccaa gtcttggtcg
    20461 acttgttccg ctgcgctcgt tgggaccgtg acgtaccagc gcgtataggc cgttcgagcg
    20521 aggtactcgg gccccttctc gccggtcgtc ttgtatagct caagtttgcc gagcggccgc
    20581 gttccggcta ggtactccac catcgcgatc ggctcgagat tgcccgtcgg cttctcgacg
    20641 aagttgatgg ctcgcaggcg gccgagcttg gtcatccaat tcagagcggt ctcgtccgcc
    20701 ttggtcgggg tcgccgcgtc cgcccacgcc tctttctttt cgggcatccg caccagctcg
    20761 cgcgatttga cggccttgct gatgcggact cgcttgactt cttcgtcctc gaagccctgt
    20821 agattgtgcg aaagcagccg cgagtcagcg aagttgaggc tctgcaccag atccgattcg
    20881 atcgcgaaca cctcattcga aggggcggcc ttggcgtagc gttcggtgcc acccggcgtt
    20941 tgcccgccga tcgtgagcac atgttccttg ccggccacct tcactttgat cgtgccttct
    21001 ggcttatcga aaccgaactc cgcgttgcgg gccgcttcta ctttaccaat cgcgcgcacg
    21061 gccttgagcg ccgccacctt ctcgatgaac tgctctgcct ctttggttcc aatgaagagc
    21121 tggctctcgc gcttgacggg cggcgggagc ggcggcgtgg agctatctgc aggctttgga
    21181 gcaggtggct cgtcttcctt gtccaggcgc acgacgtagt aacgcccctg cgcgtcgctc
    21241 ttcggttgaa cgcgcacctt gcgcttcttc gattcgaatt cgacggattc gacggcgtcg
    21301 gcccgacctg accaaacctc gatcgtttcg ctcttcgtgc gctcggcctt ctcttctcgc
    21361 gaccaaacac tgaaagccag cgcgcccgcg accgcgagca accccaagtg cgttacgacg
    21421 cttttcattt cttgcctccg cgcccgcgag aacgccgatt gctcagcagc agccccgaag
    21481 ctagcaccag gccgggaata ccgagaatcg ttccgtaaaa ccagctcgag tccgcttgtt
    21541 tggtgtgatc gatgcggacg tcttcctccg tgttcggcaa accttgggaa atgctctcct
    21601 cgcccactag ccaacgcatc gcatcgacca gcaaaatctg attgccgatc acttcgccca
    21661 tcacgaagtc cgagaaggcg tcagcatcgg caaacaccat ggctttgaag tccttgggct
    21721 cgggcttgtt cttcttatcg tccttcttcg cgtcgctggc tgccggcggg ttcgcgagag
    21781 cgcgcgtcac cgccgcggcg aagttgaacg acacgctctt ctcggccgga gcgtcgagct
    21841 tgaagttgtg attcccatct tggaagctac ccgtcggaga cttcacggcg aaatcgaccc
    21901 gctccgagcc gccacccttc tcgagactgc cagccccgaa caccacgatc gccgagcgcg
    21961 gtgcgtttcg gctgagggtc gagaccgagg cgtgtgaaga gaacgtgttg ctcgcgagcc
    22021 gcgtgcgatc cgaatcgttg tgtcgaaccg cgaggtgctg ccgctcgttc gcgatcagat
    22081 ccggcgcgaa tttcagcccc gccagggctg caagttcgcc gttgaagccg gcgtctgcgg
    22141 cactggccga gtcagccacc gccccattcg aagcggccgc tttgccgccg acggccgccg
    22201 acggcgcggg ggacggcggc gcagccagcc cggatccttc gccgcgaacg agttcttgct
    22261 cgccaatcgc ctcgggctcg agcgcgagaa gcagacgccc accgcgctcg gcgtagcgcc
    22321 gtaaggacgc gagctcttcc gcgagaaacg gcgtggtagg ccccaggatc agcaccacct
    22381 cggcgtcatc cggcacgtct cgaccgagcc cctgacccag acccagatct cgcacgagat
    22441 agttctgccg ctgcagcagc gtgcgtacga tctgccccga gcgctccgcg ctgtccggtt
    22501 tgcttcgaga gctatcggcg agctcgccat gacccacggt gacgtacgca gtgcgacgcg
    22561 atttggcgat cttgagcagt tgctcctgga aatcgcggtc gagggtcttc agcttgtttc
    22621 gagcctgctc gagatccgta ccaatttgca tggtttgcgt ggtggtgccg cgcgaaagaa
    22681 cgatcacgcc gtcttgcatg gcacgcagct cttgcgccaa cttgggcacg agcagccggt
    22741 cgcgcacttc gaccttcaag ttcgaagcac ccgccgccgc ctgcttcagg taggtctcga
    22801 cctcccgccg cacctcgttg acctcgggga aaaaggccac cacgcggatt ggatcaccgg
    22861 tcaaagaccg cgctaccttg cgcgtagatt cgctgggctg cgaggtcttg aaatacgaat
    22921 agtcgacctt tagatcgatc ccgttcgcaa agtagacgaa taacgacccg taaacggccg
    22981 ccagcgccag caccaacccc gcggccgtcg ccgaccgaac gcggcggctc tcggggtgct
    23041 cggcgcgacg catgggcagt aacgctgtct ctgcgaacac catcggcacg acgctgatcg
    23101 cgatgagcga aatccacacc acgcgcgcga gctcttcgaa gcgcgtgcgc tggtccgtgg
    23161 tcagcttgtc cagcccgagc ttcgccgcgc cggcgtcact ggtggcgaag tagaccgcga
    23221 ggccgagcaa acctagcccc gacaacacgg cgagctgacg ctcgatgcgg cggcgttcgc
    23281 cggtggtcga gaagcgcggc gcgaagcgca gcaacgtgga tgcgagcacg gccaagagcc
    23341 cgagtccgct cagcgcggga gccaaccgct cgaaaccgag cagcacacgc tgccccaaga
    23401 acaccagcaa catcccggct acgaagagta cgaagatcca agccggtgcg ggctcacctc
    23461 ggttcgccga tgcgggcgcg ccgggggcat tcgggctggg tgcgttggat gtcatcgcca
    23521 tcggcgcgcc tccaatacct tggtcgaggc cagcaagaag aagtaagaca tgaacacgta
//
LOCUS       DASTKU010000082         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_15685,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000082 DASTKU010000000
VERSION     DASTKU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15685"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1757
                     /locus_tag="VFQ61_02825"
     CDS             102..1757
                     /locus_tag="VFQ61_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9953407.1"
                     /translation="MKTPNDVEKELRPPQGTPLAGTSSLDFAQARLGTILRGKYRLES
                     VLGMGGMAVVYQATHRNQAEYAIKMLLPQHSLVEEIRSRFLREGYAANSVKHAGTVRV
                     LDDDVTEDGTAFLVLELLHGAVCDKLCTENGGRLPVAAVCSIGLQVLDVLEAAHAKGI
                     IHRDIKPANLFVLRDGTVKVFDFGIARVRETMLSASHATGRDTLLGTPAFMAPEQAAG
                     KSSDVDARSDIWAVGATLFSLLSGATVHQGDTARELLVKLVTQPPRPLRSAAPEVPDA
                     VAQVVDRALTVNREERWHSARAMQTALEEACRTLWGDVPAPAVLANLVAATRTISTAA
                     SKDASPRPEPLKRDAEAVDVPTAAIGPTMPAQPQRPDTPAARAVVETSNPVFRERVGK
                     KPRRSLAATLGIFGLLGAASGIASFVVLRSRSTAPEPSVQSSLASGSALASAPRSSSS
                     GPTSPEPGKPEIAVVPLTESSAMLPAASANAWPGPSATLREKPSAKRTPGAKNSLVRA
                     EDAGQPKANSPSAPPVPGAARANCNPPFYYDSALNRVFKKECL"
     gene            1806..>2105
                     /locus_tag="VFQ61_02830"
     CDS             1806..>2105
                     /locus_tag="VFQ61_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953408.1"
                     /translation="MSGVVCSIGSGPALAQTPPGPGECVDSNTAAQSLVREGKFGAAR
                     EQLKRCLEPACPGLVRDDCAQRLDALDRVQPTVVFEVKDEQGRDLAEVTIQVDGQL"
BASE COUNT          389 a          631 c          712 g          373 t
ORIGIN      
        1 agttttgggg ctctaccgcc ggatcaggca ggccatgaga gcatcgagag atttggctcg
       61 cggcctgggc aaagctgcat aacaatgcgg tcgtcggacg catgaagacc ccgaacgacg
      121 tcgagaaaga gctgcgtccc ccgcaaggaa cgccgctcgc gggcacatcc agcctcgact
      181 tcgcccaggc tcggctcggc actattttgc gcggcaagta ccgcctggag tcggtgctcg
      241 gcatgggtgg catggccgtc gtgtaccagg cgacccaccg caatcaggcg gagtatgcga
      301 tcaagatgct gctgcctcag cactcgttgg tggaggagat tcgcagtcga ttcctacgcg
      361 aaggctacgc agccaattcg gtcaagcacg ccgggacggt gcgcgtgctc gatgacgacg
      421 tgaccgagga tgggacggcg tttctggtgc tcgagctgct ccacggggcc gtgtgcgaca
      481 aactctgcac ggagaacggt gggcgcctgc ccgttgcagc ggtgtgtagc atcgggcttc
      541 aggtgctcga tgttctggag gcggcgcacg caaaaggcat cattcaccgc gacatcaagc
      601 cggcgaacct gttcgtgctg cgcgacggaa cggtcaaggt gttcgacttc ggcatcgcgc
      661 gcgtgcgaga gacgatgctc agcgcgagcc atgccacggg gcgtgacacg ctgctcggaa
      721 caccggcgtt catggcgccc gaacaagccg ccgggaaatc gagtgatgtg gatgcgcgct
      781 ccgatatttg ggccgtgggt gccacgcttt tttccctgct gagcggggcg accgtacatc
      841 aaggggacac cgcgcgagag ctgttggtga agctcgtgac tcagcctccg cggccgctgc
      901 gctcggccgc gcccgaggtg cccgatgcgg tagctcaggt cgtggatcgc gcgttgaccg
      961 tgaaccggga ggaacgctgg cactcggctc gagccatgca aaccgcgctg gaggaagcct
     1021 gccgcacttt atggggggat gttccggcgc ccgcggtgct cgctaatctc gtggcggcga
     1081 cgcgcacgat ttccacggcg gcgtccaagg acgcgtcgcc ccgcccggag cctttgaagc
     1141 gtgatgcgga agcagtcgac gtacccacgg ccgctatcgg tccgacgatg cccgctcaac
     1201 cgcaacgccc cgatacgccg gccgcgaggg ccgtagtaga aacatcgaat cccgtgtttc
     1261 gggaacgagt ggggaagaag ccccgacgat cgttggctgc cactctcggc atttttggat
     1321 tgctcggggc ggccagcggc atagcttcgt tcgtggtttt gcgaagtagg tcaacggcac
     1381 cagagccgag tgtgcagtcg tcgttggcgt ccgggtcagc tttggcgagc gcacctcggt
     1441 ccagcagttc cgggccaacg agtccagagc ccgggaagcc agaaatcgcc gtagtgcctt
     1501 taaccgagag cagcgcaatg cttcccgcag cttcggcgaa tgcgtggccg ggtccaagcg
     1561 cgacgcttcg agaaaagcct agtgccaagc gaacgcctgg cgcgaaaaac tcgttggttc
     1621 gtgcagagga cgccgggcaa cccaaggcaa attcgccttc tgctccgccg gtgccgggag
     1681 ctgctcgtgc caactgcaac cctcccttct attacgattc ggcgctcaat cgcgtcttca
     1741 agaaagaatg cctctaggcg ggtgagaata ccgtgttttc ccagcgaagg ataaaggcaa
     1801 caatcttgag cggcgtggtc tgctcgatag gcagcggtcc ggctttggcg caaactccac
     1861 ctggccccgg tgagtgtgtg gactccaaca cggccgcgca gtcactggtg cgagaaggca
     1921 agttcggggc tgcccgtgag caactcaagc gctgtttgga gccggcttgt ccgggtttgg
     1981 tgcgggacga ctgcgcgcaa cgcttggacg cgctcgaccg cgttcagccg accgtagtat
     2041 tcgaggtcaa agacgagcag ggccgggact tggcggaggt gaccatccag gtggacgggc
     2101 agctg
//
LOCUS       DASTKU010000083         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_684552, whole genome shotgun sequence.
ACCESSION   DASTKU010000083 DASTKU010000000
VERSION     DASTKU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684552"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            630..1673
                     /locus_tag="VFQ61_02835"
     CDS             630..1673
                     /locus_tag="VFQ61_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953409.1"
                     /translation="MLACSSTGSSSEGSGGSSSTGSGGQGGSIGSGGSIASGGSSTGS
                     GGSGKGGATGKGGDTANGGGPSNGGSGAGGANTGGSGNSGGNSAGSGNTAGAAGSSGT
                     CPTDAEEKFSFFILSNAALIRESGKADGFGGGLGGIAGADKICQKVAEYVSPCQSKKV
                     WRAFLSTTTENAIDRVGTGPWYDRKGRLLASTKEMLVMDRPGDADVAIKNDFPNENGT
                     LNRNPDGTGQVDNHETLTGTGIDGKLYNQDPSGTGSMACGPDVGASGQGTWSAERATC
                     WDWTRATEEGCPRVGHSWPRQLSGTNWMSVWNENGCAPGGTLTDTGAPMGRKVGAYGG
                     YGGYYCFAVIPHP"
     gene            complement(1769..2188)
                     /locus_tag="VFQ61_02840"
     CDS             complement(1769..2188)
                     /locus_tag="VFQ61_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9953410.1"
                     /translation="MKAQLKIANKNVAYWVVTGLFCLQMGFTAYAQLRLPQVADAFQH
                     LGFPDYFRVELSWAKLLGVVLLLVPVPARLKEWVYAGFAFDLASAILAHVAVGDGVKA
                     WGWAAGTGMLWGVSYVLWRTLRPAESNVRAELSARAI"
     gene            complement(2273..>2908)
                     /locus_tag="VFQ61_02845"
     CDS             complement(2273..>2908)
                     /locus_tag="VFQ61_02845"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HET9953411.1"
                     /translation="IRRELCDEALRLSLMLREDPAGASPDADALIALMCFHAARFEAR
                     VDVMGGLLLLEEQDRALWDHELLERGLSHLTRSARGQSLSRYHVEAGLAAQHCLAPSY
                     AETDWEEIVRLYDVLERIAPSPLNVLNRAIALAEWQGADVALAALQGFEAPSWLLGYY
                     LWDATLGELYRRRGDSEQALMHTERALSAAPTNPEKALLERRIQKILASKK"
BASE COUNT          586 a          902 c          949 g          471 t
ORIGIN      
        1 caggatcgct gatcacggtg agcaccaaga cacgcgtcca gaggtgcttg cgataccaaa
       61 gcccgcgcgg gttcaaatgc ttggcaaccg cctttgctct gtgcccacga ggtatgcttg
      121 aacaccttcc gcaggtaaat cgcgagtccc tgaatgctgc gcttgcgagg cgcttagcag
      181 gcgttgaaaa aacgccctgc gtcatgacag cccaccccgc ggcacacacg acgcgcagaa
      241 gcaagcatca cggaacctca gagtcatggg ctttcgtgat cggcgcgcgc agtatgattc
      301 atccattcgc gggcccttac ttgtgcgcgc gcttccgtcg cgttctgcat gaagcgtgag
      361 ccatgcgtga cgacaatggc cgtgaccccg agctagtacg aagagtcgag ggagctgccg
      421 gcgggcggaa aatcagcctc gatttggtga caacatttcg acgaattgat tactagaagg
      481 agacaggagg aagacgcacc cgcgccttct ttgattgcag cgctgaaccc caccaccacc
      541 cacaagcggc caaagtgaat aagtccctcc ttcacgtcct tcgcgaagtc gatttttcct
      601 cagtcactgt tcttgcggcc ttgcctctcg tgctcgcctg ctcgagtact ggaagctcga
      661 gtgaaggtag cggaggcagc agcagcacgg gctcgggcgg acagggaggg agcatcggct
      721 ccggtggctc gatcgcgtcc ggaggaagct cgaccggcag cggtgggagc ggcaagggcg
      781 gcgcaactgg caaaggcggg gacaccgcca acggtggagg accgtccaac ggaggctcgg
      841 gcgctggcgg cgcaaacacg ggaggctcgg gcaacagcgg cggcaacagc gccggttccg
      901 gcaacacggc gggtgcggcg gggagcagcg gaacctgccc taccgatgcc gaggagaagt
      961 tcagcttctt catactaagc aacgccgccc taattcgtga gtccgggaag gccgatggct
     1021 tcggcggcgg actgggtggc atcgcgggag cggacaagat ttgccagaag gtcgctgagt
     1081 acgtatctcc ctgtcagtcg aagaaggttt ggcgcgcgtt cttgagcacg acgacggaga
     1141 acgcgattga tcgggtcgga accggtcctt ggtacgatag aaaggggcgg ttgctcgcga
     1201 gcaccaagga aatgctcgtg atggatcgac cgggcgacgc cgacgtcgcc atcaagaacg
     1261 actttcccaa cgaaaacgga acgctcaatc gcaatcccga cggtacgggc caggtcgaca
     1321 accacgagac gctgaccggt acgggcatcg acgggaaact ctacaaccag gatccttccg
     1381 gcactggctc gatggcgtgc gggcccgacg tcggtgcgag cggacaggga acttggtcgg
     1441 ccgagcgcgc cacgtgctgg gactggacgc gcgcaacgga agagggttgc ccgcgggtgg
     1501 gccattcgtg gccgcggcag ctcagcggga cgaactggat gtcggtatgg aacgagaacg
     1561 gttgtgcgcc gggcggcacg ctcaccgata ccggcgcacc catggggcgc aaggttggcg
     1621 cctacggtgg ctacggcggc tactattgct tcgccgtcat cccgcatccg tgacacgccg
     1681 ccgcgcggct cgtcgccgga ttggattgca agctcgcgca tgtgacaacg ccgcgacgtc
     1741 gccaagtccc tgcagcgagc ggattcggct aaatggcgcg agcgctgagc tcggcgcgca
     1801 cgttcgactc cgcagggcgc agcgtcctcc aaagcacgta cgatacgccc caaagcattc
     1861 ccgtacctgc cgcccagccc caggccttga cgccgtcgcc cacagcgacg tgagcgagga
     1921 tcgcggaggc gaggtcgaaa gcgaagccgg cgtagaccca ttctttcaat cgcgccggca
     1981 ccggaaccag caacaacacc acacccaaga gcttcgccca cgagagctcg acgcgaaagt
     2041 agtcagggaa gccaaggtgt tggaacgcat cggccacttg cggcagtctg agctgcgcat
     2101 acgcggtgaa gcccatctgt aagcagaaca ggccggtcac gacccagtaa gcaacattct
     2161 tgttggcgat cttgagctga gctttcatcg ttcgaacctc cgggaacgag cgcgcttcgt
     2221 cgcggctctg ttcgtaggac gcgggcgcgg cccggaatgg acactcgcga gcttattttt
     2281 tcgaagcgag gatcttttgg atgcgccgct cgagcagcgc cttttctggg ttggtcggtg
     2341 ccgcagagag ggcacgctcg gtgtgcatca gcgcctgctc gctatcgccg cgccgccgat
     2401 acagctctcc gagggtggcg tcccacagat agtacccgag caaccaactc ggcgcctcga
     2461 agccttgcaa agcggcgagc gcgacgtccg cgccttgcca ctctgcgagc gcgatcgccc
     2521 gattcaatac gttcagcgga gagggggcaa tgcgctcgag cacgtcgtag agccgaacga
     2581 tctcttccca atccgtctcc gcgtacgatg gggccaggca gtgttgcgcc gcgaggcccg
     2641 cctcgacgtg atagcgcgat agcgattggc cccgggccga gcgggtcagg tgcgaaagcc
     2701 cgcgctcgag gagctcgtga tcccagagcg cgcggtcctg ctcttcgagc aacaagagac
     2761 cgcccatgac gtcgacgcgc gcttcgaagc gcgcggcgtg aaagcacatc agcgcaatca
     2821 gcgcgtcggc gtccggcgac gcgccggccg ggtcctctct cagcatcaac gaaagtcgca
     2881 gcgcttcgtc acaaagctcg cggcgaat
//
LOCUS       DASTKU010000084         8852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1354397, whole genome shotgun sequence.
ACCESSION   DASTKU010000084 DASTKU010000000
VERSION     DASTKU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8852
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354397"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFQ61_02850"
     CDS             complement(<1..196)
                     /locus_tag="VFQ61_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953412.1"
                     /translation="MATPAFEEREVAVLVGALLLVTCTLYFLRDFKRVPRWPLLSLAM
                     CSLVLGNIATVVEHFIAFSFF"
     gene            complement(298..927)
                     /locus_tag="VFQ61_02855"
     CDS             complement(298..927)
                     /locus_tag="VFQ61_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953413.1"
                     /translation="MSVWKVEELRMPPVHVTLAPPPPEPPPFLRRADAPKPAGGTAAK
                     TPKLLQPTPHRAQTPAEPEPTAQNTANTPDASDTDASSGVPGGDPSSTQTWLDPPPAP
                     TSTPQRVPVVQVTPQIARHLLLVDPNVEPYRVPVPRALQRSGVDLSQTIVKLCADARG
                     AVTQVTIVKGGIAAIDAQLPTVLRRWRYRPMRVDGTAVDFCYVFRYDLR"
     gene            complement(1141..2433)
                     /locus_tag="VFQ61_02860"
     CDS             complement(1141..2433)
                     /locus_tag="VFQ61_02860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9953414.1"
                     /translation="MRVLTVVGNLDVRGTQRAALSYTLAYQRAGHEVGVLAHSGGGPR
                     EQLLQRHSIPAFIGGVEPLLDSALREAEAFRAEVVHIHRAGPFNQKELRILRALRRNN
                     IVIETNVFGRVDYSPDAHLVDAHLHISAFCLWRWHRWLGRRPQVGVLVPYPVESDNFK
                     RVSDEERSRQRAAWGIPDDAYVCGHVGFKLSKCVIDGFVALTRKTSKAWLVCVPFTER
                     LAEYVRLLPESVRARIVNVGQISDDAELQRAYSAFDCLLHAAEIGESFGYVLAEAQLC
                     GVPVVTLSRPHRDNAQVELVRHRVGGLVAGQSACFGDAVVELYEDTALRTQIRSTAPA
                     RMHQLYDVERVTALALRVIDTLKAHPDARARATALELDPHLRTRVADEVILGSLRDTV
                     GGPSLSDLAMMRMLTHERSQQMVRMIRHREFPRLRISS"
     gene            2535..3773
                     /locus_tag="VFQ61_02865"
     CDS             2535..3773
                     /locus_tag="VFQ61_02865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9953415.1"
                     /translation="MTTRKGMDYSFRVSLPASIGLVIPNFNGERFLPATLESVVSQNY
                     PRLRCILMDGGSRDSSLTIAREYQRKYRGIEIYSERDEGQADAIQKGFDRLDTELVGW
                     LNSDDALLPGTLHRIAQARELHPDAALFYGNLELCDEFGRLFSVLRARDLDYDTLRTG
                     RGVIAQPGSFYSRELLKQAGGVRREFWGLMDLDLWLRLLQRGEAVRLRDRVAQFRVHA
                     SAKSSELPVQYYREALRITWRYNAGQRLRALTPRVLGIPLHFARWLNGTYLRSRPRLV
                     EPTTPLRIELPAVLLPSSVAEVLQNEPLFASANSPGNPGAEPAVRFVDVETVLHSQRG
                     FRTGREFRSPVVAVTTTLSERQASELVALRAPLAFVLAGSAAAQCLIAQGFPAFRVGD
                     RAHAPDWQRGLEVALAATPR"
     gene            complement(3791..4615)
                     /locus_tag="VFQ61_02870"
     CDS             complement(3791..4615)
                     /locus_tag="VFQ61_02870"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET9953416.1"
                     /translation="MKVMLEPFEGTRSMSVRERVLYKLKHSLWHAKRRVPPAARLKAE
                     DLLLNWHIRPGAPSMFRALSVLKRGGFEPTQIIDVGAHHGDWAVDALELWPNSRVLML
                     EAREAERPVLERRRRESGGRAEFEICLLGPEERENAHFFELELGGTGSSVLEELSPVP
                     RQKKVLPMRRLDNVARQRDFQAAQLLKLDVQGFELQVLEGAKEVLSRAEVVVSEVALL
                     PYNQGAPLMAEVIDYFDKRDFQLFDLGSIMRDRAQRAVQVDALFVRRDSPLRNRTS"
     gene            5031..6113
                     /locus_tag="VFQ61_02875"
     CDS             5031..6113
                     /locus_tag="VFQ61_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953417.1"
                     /translation="MRIQTSSMGTSWPVATLGVQLAGFALALTISAQAQPSPAPPEPN
                     PAQLSPMQASPVQAQPSPVQAQPSPVQTPPNPAPMSASVEPAFPGSNSESSALVSTNS
                     VAADPPGANPDLEVRTIVDFGGLLPLYHHIQLGREGTEFNYVKRGGQDVIFPVGRLSV
                     EVAARRHRITFLYQPLELNTEVVLARPLTVDGLTFSEQTPINLRYGFPFYRVSYTYDV
                     APSAAWRVALGGSFQLRDATITFTSADGSARRTRRDVGPVPALKASLRYDARSRWFGA
                     FEVDGFYAPVRYFNGGDSDVEGAIIDANLRGGFRLSPRVDAFLNLRWLGGGAEGSSEP
                     EDFSDGYNENWLHFLIVSLGVDFGLI"
     gene            complement(6117..6704)
                     /locus_tag="VFQ61_02880"
     CDS             complement(6117..6704)
                     /locus_tag="VFQ61_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953418.1"
                     /translation="MFTQREPLWSRLREVREVLMFTFDFELLEQPLDSGAYLSPAPWV
                     PAFVVPNADALVIGRDATGGVYVACDFPESHTGYCLHLDTRGHAVNLGADLEHALALI
                     LALPYWDQLLLESPSGNVDELRALALELEREVCDDLPALPRARADLQSFLELPTLDDP
                     VGRLHRLALTPTVQVLNPHGWIYASPLASGAAHPA"
     gene            7293..>8852
                     /locus_tag="VFQ61_02885"
     CDS             7293..>8852
                     /locus_tag="VFQ61_02885"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9953419.1"
                     /translation="MKRIKRLPSLILSSVSLALALGCQAEIVEPAGSSGATGSVSPSA
                     GSGSTQTGMPGAGASSNGSAADTGSIGGGSRGGATSSGGSSGALATGGSVATDVNRVP
                     IHRLNNAEYNNTLADLLGITSKPAAGFIDDEKLAGFDTIAAAFGMTDARYEQYFDAAD
                     TAVEQAFADATLRDRILVCKPADATDPTCARQILSAFVKRAYRRPIQSAEIDGLLAVV
                     SGASALGEDFAGSIRQALKAVLSSAEFLYRVEFDAEPSSPAAHPLSAHELGSRLSYLV
                     WSTLPDTALIERADRGDLSSDAAVAAELDRLLADPRSARFVESFAGQWLGLRQLGSHQ
                     VDASLFPDWNETLRAAMVQEGLQFFTRLLRDQPISEFFTSEQHSVNAPLAKLYGLPAV
                     SGDQWLDVSTPIPGRRGFMGLAGFLTLTSFSKRTAPTLRGKWVLENLLCNEVPAPPAN
                     VPELDKAESSGAQSENVRARLEEHRKNPSCAGCHQVLDPIGLGLENFDAIGRYRTAYT
                     GGEPVDASGQLP"
BASE COUNT         1618 a         2745 c         2864 g         1625 t
ORIGIN      
        1 tgaagaagct gaacgcaatg aaatgctcga ctacggtggc gatgtttccg agcaccaacg
       61 agcacatggc gagactcaag aggggccagc gcgggacccg cttgaagtcg cgcaggaagt
      121 agagtgtaca cgtcactagc agcagcgctc cgacgaggac ggcaacctcc cgctcttcaa
      181 aggccggtgt agccattagc ctatggaata ggcgtgaatc ggcgcggaca caagcgccac
      241 actcgagtgc tcctcgagac cacaccctaa tgaaagcggc gcgcggagct ccgaattcta
      301 gcggaggtcg tagcgaaaca cgtaacagaa gtcgaccgca gttccgtcga cacgcatggg
      361 acgatagcgc caacgtcgaa gtacggtcgg aagctgggca tcgatcgccg cgattccccc
      421 tttgacgatg gtcacttgag tgactgcgcc gcgcgcatcg gcgcacaatt tgacgatcgt
      481 ttggctcaag tccactccgg atcgctgcag cgcgcgaggc accggaaccc ggtacggttc
      541 gacgttcgga tccaccaaca acagatgacg ggcgatctgc ggggtgacct gaactaccgg
      601 cacccgctgc ggagtggaag taggtgcggg cggcgggtcg agccaagtct gggtcgaact
      661 cgggtcaccg ccgggcacgc ccgagcttgc atcggtgtcc gaggcatcgg gagtattcgc
      721 ggtgttttgc gcggtgggtt ccggttccgc gggagtttgc gctcgatgcg gagtgggttg
      781 cagcagcttt ggagttttgg ctgcagttcc ccccgcgggc ttcggggcgt cagctcttcg
      841 aagaaaaggc ggcggctctg gcggcggagg cgcgagtgtg acgtgaacag gtggcatgcg
      901 aagttcttcc accttccaaa cggacattcc caaacccacg cacagcgcgg ccgcatggac
      961 cgcaatggat aggggcacca ttacgcgtcg gccctggact ggactcgaat gctgaagact
     1021 caatactcca tcgaacgcca tacccatcca atgttcgttg ccgggcaaat gcgtccatca
     1081 gattggccgc gctcacggga tcacgatgga taggggtctt tccgttcggg aacggggcgc
     1141 tcaagaggag atcctcaatc gagggaactc gcggtgccga atcatgcgca ccatctgctg
     1201 gctgcgctcg tgcgtgagca tccgcatcat ggccaagtcg ctcagcgaag gtccaccgac
     1261 ggtatcgcgt agagagccga ggatgacctc gtcggctaca cgcgtgcgca ggtgtggatc
     1321 cagctcgagc gccgtggctc gggcgcgagc gtctgggtga gccttcagcg tgtcgattac
     1381 cctcagcgcc agggccgtca ctcgctccac gtcatacaac tgatgcatcc gcgcgggtgc
     1441 cgtgctgcgg atctgcgtcc gcagcgccgt gtcttcgtag agctccacca cggcgtcgcc
     1501 gaagcatgcc gactgccccg cgacgagccc tcccacacga tgccgcacca gctcgacctg
     1561 agcgttgtcg cggtgtggtc tcgacagcgt taccaccggg accccgcaca gctgcgcctc
     1621 cgccagcacg tagccaaagc tctcgccgat ttccgcggcg tgcagcaggc agtcgaaagc
     1681 cgaataggcc cgttgcagct cggcatcgtc gctaatctga cccacgttga cgatgcgggc
     1741 ccgcacactc tcgggcagca agcgtacgta ctcggcaagc cgctccgtga acgggacaca
     1801 taccagccag gctttggatg tcttacgagt cagggcaacg aacccgtcga tcacacactt
     1861 actgagcttg aaaccgacgt gaccacagac gtaagcgtca tccggtattc cccaagcggc
     1921 gcgctgtcgg ctgcgctcct catcggacac gcgcttgaag ttgtcggact ccacgggata
     1981 gggaaccagc accccaacct gaggccgtcg ccccaaccag cggtgccagc gccacaggca
     2041 aaacgcgctg atgtgtagat gggcatctac caagtgtgcg tcgggcgaat agtcgactcg
     2101 accgaacaca ttcgtttcga tcacgatatt gttgcggcgc agggccctga gaatgcgtag
     2161 ctccttttgg ttgaatggcc ccgcccgatg aatatgtacg acttccgcgc ggaacgcctc
     2221 ggcttctcgc aacgcagagt cgagcagcgg ttcgaccccg ccaatgaagg ctggtatcga
     2281 gtgtctctgc agtagctgct cgcgggggcc accgccgctg tgggccaaca ctccgacctc
     2341 gtgcccggct cgctgatacg cgagtgtgta cgagagcgcc gctcgctggg tgccgcgaac
     2401 atccaggttg cccacgaccg tcaaaacacg cattcacata gctccgttcg gtggctacct
     2461 tacgttagct ggtcatccgc tgctagctcg atgaacctag aaaaagaccc tgtgtccgag
     2521 aacgggcgct gctcttgacc acccgaaagg gcatggacta ctcgtttcgg gtgagcctcc
     2581 cggcatcgat cggtctcgtc attcccaact tcaacggcga gcgcttcctg ccggccacgt
     2641 tggagagcgt ggtgagccag aactaccccc ggctgcgctg catcttgatg gatggcggtt
     2701 cgcgcgactc ctcgctgacg atcgcacggg aatatcagcg taagtatcgt gggatcgaga
     2761 tctacagtga gcgggacgag ggccaagcag acgccattca gaagggcttc gatcggttgg
     2821 acacggagct ggtgggctgg ctgaactcgg acgacgcgct ccttcccgga accctccacc
     2881 gcatcgcgca ggcccgcgag ctacacccgg acgcggccct gttttatggc aatctcgagc
     2941 tgtgtgacga gttcggtcgg ctcttctccg tgctgcgagc ccgcgatctc gattacgaca
     3001 cgctccgcac cggacgcggc gtgatcgcgc agccgggttc attctacagt cgcgagttgc
     3061 tgaagcaggc tgggggagtg cgccgggagt tctggggact gatggacctg gatctttggt
     3121 tgcggctcct gcagcgaggc gaggcggtac gcttgagaga tcgcgtagcg caattcagag
     3181 tgcatgcgtc cgccaagagc agtgagctac cggtgcaata ctaccgagag gctctgcgta
     3241 tcacttggcg ttacaacgcg ggccaacgcc tgcgcgcgct caccccgcgg gtgctgggca
     3301 tccctctgca tttcgcgcgc tggctgaacg ggacctatct acgcagccgt ccgcgactcg
     3361 tagaacccac gaccccactt cgcatcgagc tcccggcggt cctgctgccg agcagcgtgg
     3421 cggaggtgct ccagaacgaa ccactcttcg cgtcggctaa ttctccgggt aacccgggcg
     3481 ctgagccagc agttcggttc gtggacgtgg agaccgtctt gcactcccag cgtgggttca
     3541 gaactgggcg cgagtttcgg tcccccgtgg ttgccgttac gacgacgctt tccgagcgtc
     3601 aggccagcga gctcgtcgcg ctcagggcgc cgttggcttt cgtactcgca ggcagcgctg
     3661 cggcgcagtg cttgatcgcg cagggctttc cggcatttcg agtcggggac cgcgcgcacg
     3721 cgccggactg gcagcgcggt ttggaagtcg cgctggcagc cacgccgcgt tagcttcgtg
     3781 gtcttcgagg ctacgacgtt cgattgcgca gcggtgagtc gcgacgcacg aacagggcgt
     3841 cgacctgaac ggcgcgctgg gcacgatctc gcatgatgga tcccagatcg aagagctgga
     3901 agtcgcgctt atcgaagtag tcgatcacct cggccatcag cggcgcgccc tggttgtagg
     3961 gcaggagcgc cacctcactg accaccactt cggctcgcga caagacctcc ttggctccct
     4021 cgagcacctg gagctcgaag ccttgaacgt cgagcttgag cagctgcgcc gcttggaaat
     4081 cccgctgtcg ggctacgtta tccagccgcc tcatcggcaa caccttcttc tggcggggca
     4141 cgggtgaaag ctcctccaaa acggaggacc ccgtgccgcc caactcgagc tcgaagaagt
     4201 gtgcattctc ccgctcctcg ggaccgagca gacaaatctc gaactccgct cgacctccgc
     4261 tctcgcgtcg tcggcgctcg aggaccggcc gctccgcctc gcgcgcttcg agcatgagca
     4321 cgcgcgagtt aggccatagc tccagcgcgt ctaccgccca gtctccgtgg tgagcgccca
     4381 cgtcgatgat ttgggttggt tcgaagcccc ctctctttag taccgataag gcgcggaaca
     4441 tcgagggggc ccccggccga atatgccagt tgagcagcag gtcttcggcc ttcaatcggg
     4501 ccgcgggcgg aactcgccgc ttggcgtgcc aaaggctgtg tttcagcttg tacaacactc
     4561 gctctcgaac ggacatgctt ctggtacctt cgaacggttc gagcatgacc ttcatgctcg
     4621 aaccatgcgc tagcacagcg gcgcgctcgc atgggagcat atgcatgaag acgcgccggc
     4681 tctctggctc gcgggccgtg cgaccatcgg accgcgtgag gctcagcgct ttggtagggc
     4741 cgcacgccgc gcctcccacg aggcgctagt gagctggccg cagcgagtgt gctcgtgtcg
     4801 atgttcatag cgtgggtgaa tgcgaattgc acgactcacg cacacgtgcg cgaaaaacac
     4861 cgagtggtgt cccggtagac gaagagcccg tcggcaagac gggcttggtg tcgtgtcggt
     4921 gatctctgca tcgcggcgta acttcgctgc acccagggcc caaaacgcgc gcggcattcg
     4981 acgccactca gccatgcgac tgcgtggcat cgccacttga cgttcaggtc atgcgaattc
     5041 agacatcgag tatgggaacg tcatggcccg tcgccacgct cggagtgcaa ctcgctggct
     5101 tcgctttggc gctcacgata tctgcgcaag cgcagccgag ccccgcgccg ccggaaccga
     5161 accctgcgca gctgagccct atgcaagcga gccctgtgca agcgcagccg agccctgtgc
     5221 aagcgcagcc gagccctgtc caaacgccgc cgaaccctgc gccaatgtcg gcgagcgtcg
     5281 agcccgcgtt ccccggctcg aattccgaat caagcgcgtt ggtatccacg aactccgtgg
     5341 ctgcggatcc ccccggagcg aaccctgacc tggaggtccg cacgattgtg gacttcggtg
     5401 ggttgttgcc gctctatcat catatccagc tgggccgcga aggtacggag ttcaattacg
     5461 tgaagagggg cgggcaagac gtgatctttc ccgttggccg tttgagcgtc gaggtggcgg
     5521 ctcggcgaca ccggatcacg ttcttgtatc agcccttgga attgaacacc gaggtggtgc
     5581 tcgcgcgccc cctgacggta gacgggctca ccttttcgga acagacccca atcaatctgc
     5641 gctacggctt tccgttctat cgcgtgagct acacctacga tgtggccccg agcgcggctt
     5701 ggcgcgtggc gctcggtgga tcctttcagc tgcgcgacgc gacgatcacg ttcacgtccg
     5761 ccgacggcag tgcgcggcgt acccgccgtg acgtcgggcc agtgcccgcg ctcaaggcct
     5821 cactacgcta cgatgcacgc tcgcgctggt tcggggcctt cgaagtggac gggttctacg
     5881 cgccggtgcg ctacttcaac ggaggcgact cggacgtgga gggcgcgatc atcgacgcga
     5941 acttgcgggg gggcttccgt ttgagcccca gagtggatgc attcctgaac ttgcgttggc
     6001 tgggcggtgg cgccgagggg agctcggagc cggaggactt cagcgatggc tacaacgaga
     6061 attggctgca cttcttgatc gtgagtctcg gcgtcgactt tggactgatc tgagactcac
     6121 gccgggtgcg cggctccgct cgcgagcgga gaagcataaa tccagccgtg cgggttcaga
     6181 acctggaccg tgggggtgag cgcgaggcgg tgcagacgac ctacggggtc atccagcgtc
     6241 ggcaactcga ggaagctctg cagatcggcg cgagctcgag gaagcgcggg taaatcgtcg
     6301 caaacctcac gctcgagttc gagcgcgagc gctcgcaact cgtcgacgtt acccgaggga
     6361 ctttcgagca ggagttggtc ccagtacggc aaggcgagga tcagagcgag cgcgtgctcg
     6421 aggtctgcac ctagattcac ggcgtgaccc cgtgtatcca ggtgcaggca atagccggtg
     6481 tgcgactccg gaaaatcaca cgcaacgtag acgcctccgg tcgcgtctcg cccaatcacg
     6541 agcgcatcgg cattcggcac gacgaacgcg ggcacccaag gggcggggct gagataagca
     6601 cccgaatcga gcggttgctc gaggagctcg aagtcgaacg tgaacatgag cacttcacgc
     6661 acttcgcgga ggcgactcca gagcggttcc cgctgggtaa acatgccggt gattcagtag
     6721 ctacccaaag aacactcatg gagcaaacgc ggcgggactt tttctcgacg cgcatttctg
     6781 gtcgtgcatt cctgccccta gtatcggctc caagcggcgt gggatcttct gccggatttg
     6841 ggtagctcca acttcggggg ccggcgcggt acggtgggag aggtttaggg cttgggcggc
     6901 gctgccgacg acagtcgacg ggatgtcgcg cccgttgcgg tatggcccga tccgaagcgc
     6961 gtggggacgt cttacccggg agtgacgcaa aaccttgctc gctctgtaac aggggcccgc
     7021 tgcgctcttc gggaccgcac gtcctcggga gcggctcgat gcgtcgaacc gacgttgcgg
     7081 cgcgcccgtg gacgataccc ccgagggttt caccgcgatc atcgtttggc ggtctgacct
     7141 tcgaattcga gaggtgtgcg gatgtcgcga ggcgtcctcc cttcgtgagc gtgactcgat
     7201 tttcgaaaac gaaagctcac ggggggcacc actaccgaga ccaccagaac gcgagtttcg
     7261 aaccgcgtcg ggtgagcctc gagagagtac gcatgaagcg aatcaagcgt ttgccttctc
     7321 taatactgag cagcgtgagc ctggctctgg cgctggggtg ccaagccgag attgtggaac
     7381 ccgcaggctc gagtggcgcg acgggatcgg ttagcccaag cgccggctcg ggaagtaccc
     7441 agacgggtat gcccggcgca ggggcttcct ccaacgggtc ggcggccgac acgggctcga
     7501 ttggcggagg gtctcgaggc ggtgcaacca gtagcggggg ttcgagcggc gcgctggcaa
     7561 ccggtgggag tgtcgcgacc gatgtgaatc gggtgccgat ccaccgcttg aacaacgccg
     7621 aatacaacaa caccctggcg gacctgctcg gcatcacctc gaagcccgcg gccggcttca
     7681 tcgacgacga aaagctcgcg gggttcgaca cgatcgcggc ggcgttcggg atgacggacg
     7741 cgcgctacga acagtacttc gatgccgcgg atacggccgt ggaacaggcc ttcgccgacg
     7801 cgacgttgcg cgatcgcatc ttggtttgta agcccgcgga cgcgaccgac ccgacgtgtg
     7861 cgcgccagat tctctccgca ttcgtcaaac gcgcctaccg gcgcccgatc cagagcgcgg
     7921 aaatcgatgg cctcttggcc gtggtttcgg gggcgagcgc tttgggcgag gacttcgcgg
     7981 ggtcgattcg tcaggcgctc aaggcggtgc tctcttcggc cgagttcctg taccgcgtgg
     8041 agttcgacgc cgagccgagc tcgcctgcgg cacatccctt gagtgcgcac gagctcgggt
     8101 cgcgactgtc ttatttggtt tggagtacgc tgccggacac agccctgatc gagcgagcag
     8161 accgcgggga tctcagctcg gatgcggccg tggccgccga gctcgaccgt ttgctggccg
     8221 atccacgatc ggcacgtttc gtggagagct tcgcgggtca atggctcggg cttcggcagc
     8281 tcgggagcca tcaagtggat gcgagccttt tccccgactg gaacgagact ctgcgggcgg
     8341 caatggtgca ggaagggttg caattcttta cgcggctctt gcgtgatcag ccaatctccg
     8401 agttcttcac cagtgagcag cacagcgtga atgcgccgct ggccaagctg tatgggctgc
     8461 cggcggtgag tggagatcaa tggctcgacg tgagcacccc gatcccgggc cgtcgagggt
     8521 tcatgggtct ggccgggttc ctgacgctca cctctttttc gaagcgtacc gcgcccaccc
     8581 taaggggtaa gtgggtactc gagaacttgt tgtgcaacga agtgccagcc ccacccgcaa
     8641 atgtgccgga gctggacaaa gccgagtcgt ccggcgcgca aagcgagaat gttcgggcga
     8701 ggctcgagga gcatcgcaag aatccgagtt gcgccggctg ccatcaagtg ttggatccaa
     8761 ttggcttggg gctcgagaac ttcgacgcca tagggcgcta tcgtacggcg tacaccggcg
     8821 gtgagcctgt agatgcctcc gggcagttgc ca
//
LOCUS       DASTKU010000085        10178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1800134, whole genome shotgun sequence.
ACCESSION   DASTKU010000085 DASTKU010000000
VERSION     DASTKU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10178
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800134"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFQ61_02890"
     CDS             <1..203
                     /locus_tag="VFQ61_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET9953420.1"
                     /translation="YHVYEGAAMDLARKLPQACYGPKQALSTGRKRAAGLNSPSAQAW
                     ALRDAFDGLLGVVARRLKHGKD"
     gene            complement(200..658)
                     /locus_tag="VFQ61_02895"
     CDS             complement(200..658)
                     /locus_tag="VFQ61_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HET9953421.1"
                     /translation="MRWLVIGYGNPLRRDDGLGHYLARCVQRARLPHVEVQLEIELLP
                     ELSLELRRAPRVLFVDASLDPSVLGVQLTRLPATPAELSDQTRREQAPVSHVGGPASL
                     LSTCMALYGAMPEGHALAISGYDLGFGVGLSRAARRNLRRARQIAFELLR"
     gene            complement(689..2158)
                     /locus_tag="VFQ61_02900"
     CDS             complement(689..2158)
                     /locus_tag="VFQ61_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HET9953422.1"
                     /translation="MTTRIVIDPVTRIEGHAKISIMLDDSGKVSDARFHVAEFRGFEK
                     LCEGRPLAEMPGITSRVCGICPVSHLLASAKATDAILAVNVPAAADKLRRLMNLAQIV
                     QSHALSYFHLSSPDLLLGMESPPERRNLFGLAAQDPEYARGGIRLRKFGQRVIELLGG
                     RKIHPSWAVPGGVRAPLSEAARDELRELLPEALQTTQGALERFKRLLDGFGDEIANFG
                     RFPSLFMSLVAPDGSWEHYGGKLKFVDSSDRVVAEGLSAANYQEFIAEAVDFGSYMKP
                     AFYRPLGYPHGAYRVGPLARLNVCERMGTTLADRELIEYRQRFAPPQTSSFLYHYARL
                     IEMLCAVELIQRLVDDPELLSGRIRAHAEINCLEGVGVSEAPRGTLFHHYKVDENGVV
                     QRVTLLIATGQNHTAMNRTVLQIAEHYIRGPEIPEGILNRLEAGIRSFDPCLSCSTHA
                     IGQMPLEVTLLDPDGRVLAHRSRSGSDETIERSRSGSDQ"
     gene            complement(2175..2717)
                     /locus_tag="VFQ61_02905"
     CDS             complement(2175..2717)
                     /locus_tag="VFQ61_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953423.1"
                     /translation="MERVKLATLWLGGCSGCHMSFLDLDEFLLELTQFADIVYSPVID
                     RKDYPEGVDIALVEGAVANVEHLEIIAKVRERTRLLVAFGDCAVTGNVTALRNPLGHA
                     LPVLDRAYLQDVAGQPRIPGEPGIIPALLDHVLPVHEVVKVDGFLTGCPPEASRIRKL
                     IEQLLAGGAPGLSGRDLSFG"
     gene            complement(2705..3421)
                     /gene="hoxU"
                     /locus_tag="VFQ61_02910"
     CDS             complement(2705..3421)
                     /gene="hoxU"
                     /locus_tag="VFQ61_02910"
                     /inference="COORDINATES: protein motif:HMM:NF005745.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxU"
                     /protein_id="HET9953424.1"
                     /translation="MAVKTLSIDGKPIGAREDVTLLEAAREAGIYIPTLCHLPGTSNA
                     GACRLCLVEIEGSSKLAPACVTHVAEGMRVRTQSERLTSYRKMIVELLFAERNHVCSV
                     CVANGGCELQNLASVLGIDHVRYEYLHPNCEVDVSHARFGIDHNRCVMCTRCVRVCDE
                     VEGAHTWDISGRGVNARVITDLKQPWAESSTCTSCGKCVEACPTGAIFPRGTSVGELR
                     KDRGVISFLVNAREKKQWNV"
     gene            complement(3411..5066)
                     /gene="nuoF"
                     /locus_tag="VFQ61_02915"
     CDS             complement(3411..5066)
                     /gene="nuoF"
                     /locus_tag="VFQ61_02915"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HET9953425.1"
                     /translation="MDQADLDAISEKERSGSGRLHLRCCGAAGCLSSGSRAVREALEG
                     AIKQAGREQEVDVLEVGCQRLCCEGPLVEVSSGGPLYQKVKPEHAEAIVSALSGPSNE
                     LPKADTQHAFFTRQQPIVLENSGIVEPDRIESYIARGGYRALWHALREMEPRQVVATM
                     IESGLRGRGGAGYPTGLKWDTVAKQVASKRFVVCNADEGDPGAFMDRSVLESDPHRVL
                     EGMVIAGYAVGASQGYVYVRGEYPLAIRRLETAIKQAKRLGLLGSQILESPFDFKIDL
                     RIGAGAFVCGEETALMASIEGRRGTPVPRPPYPAEHGLWGYPTVINNVETFANVPPIV
                     LKGSAWFSAIGTANSKGTKVFALAGKVKNTGLLEVPMGTTLREIVEVMGGGTPNGGRI
                     KAVQTGGPSGGCIPEAHLDTPVDYDSLAALGSIMGSGGMIVMDETTDMVDIARYFMEF
                     SMDESCGKCIPCRAGTVQMHHLLQRIGRGSARSSDLVKLEELADMVKAASLCGLGQSA
                     PNPVKSTLRYFREEYTRRLNDSAEGHTTQQAKASSPEGAAHGR"
     gene            complement(5053..5610)
                     /gene="hoxE"
                     /locus_tag="VFQ61_02920"
     CDS             complement(5053..5610)
                     /gene="hoxE"
                     /locus_tag="VFQ61_02920"
                     /inference="COORDINATES: protein motif:HMM:NF005747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxE"
                     /protein_id="HET9953426.1"
                     /translation="MMRTRSAATSPSGGPARLPESPSEAVNKKDTRFEMLDSFMKRQR
                     FQPDSLIEVLHAAQELFGRLQKDVLLHVAKALKLPPSRVYGVATFYHLFSFAAPARHT
                     CVVCTGTACYVKGGDAILSVSEQELGVVAGKTRADGEVSLSTARCLGACGLAPIAVYD
                     GEVHAHQTAELAIAALKKWRQGGPG"
     gene            complement(5607..6257)
                     /gene="hypB"
                     /locus_tag="VFQ61_02925"
     CDS             complement(5607..6257)
                     /gene="hypB"
                     /locus_tag="VFQ61_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HET9953427.1"
                     /translation="MKVDIHESLLLRNQTAAEHNRSHFARRGVFCVNLMGSPGSGKTA
                     LLEATGRALAGRRTLAAMSGDLATDHDAQRLTAAGIPSCAITTGSACHLDADLVHTAL
                     HELNREFSMLFIENVGNLVCPAIYDLGQARNVVALSVTEGEDKPLKYPVMFRNADLVV
                     ITKIDLLPHLPRVDVGVIQSALERVMARPRFMKVSAETGEGMSAWFDWLSSAELNP"
     gene            complement(6307..6675)
                     /gene="hypA"
                     /locus_tag="VFQ61_02930"
     CDS             complement(6307..6675)
                     /gene="hypA"
                     /locus_tag="VFQ61_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HET9953428.1"
                     /translation="MHEYSVVESLLLRIADEARRHRAKSVSRVAVRIGRVSGVEPALF
                     ETAFRCLRENTVCAGAELQIEIVTATFQCVRCDVEFESEDPCGSLCPRCDLPARLRSG
                     DELSLERIELEVPVSEAPRE"
     regulatory      7024..7273
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            7431..9419
                     /locus_tag="VFQ61_02935"
     CDS             7431..9419
                     /locus_tag="VFQ61_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9953429.1"
                     /translation="MPLPFVYAQPATAAPVEIVVQGERAQPLAAARERQVSGSVVERE
                     RLTQPGVTVAQVLRDEPGAQTTELGAYGTWSSISLRGAPGAQTPVYLGALRLNDDLGG
                     AANLSDVPLFIVQRAELYRSHAPGFVEQLGIGGALVFEPKRPEQNQLAANITIGSYGY
                     ATAQGFAAARSGERRMLAAVELTHADNDYEFPNGNGTLFQRGDDRVTRLTNADLRQSN
                     VWLSASDQVGRAALRLTYHRANREQGALKLAVTQAKQARVATNRDAVVLESAVPLDSW
                     RGKLGVSTQAMIADTELFDPLSELSLAATRTLTRAQRVEQAGFLQHDILSGWQSTERI
                     TLGFEGLQRFEERDSRDSERSAATRSRARFSTSHQIRFGEHLSATLLGSLASYTTSGA
                     QHQGAGASDSARTSPTLTNAPQLEPEGRVGLAYRGGTRELFVNAGHYRRSATLAELFG
                     ASLLVRGNPELHSERGTTIELGARQQWLDALCERRAWLDLGSFARFSHDLIAYDTTAQ
                     GYLLPKNRKRTRALGAELSLGARPLDFLTAELDVSLVDARDVSPGGLKNELLPFVSRA
                     TVSSFLAFEQRFDADWLQRARLGLRANYKSSRFANQAGAGVIPEQLVFDLEAAATGLF
                     DALTARLRVADVFDATRFDVVGFPLPRRSFYFALESTL"
     gene            9416..>10178
                     /locus_tag="VFQ61_02940"
     CDS             9416..>10178
                     /locus_tag="VFQ61_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953430.1"
                     /translation="MKNLAIIRSVLALCAALGGAFACNVENTTHDTGGVGTKSDPESC
                     PAGVSIVLSDYMATQIALSDLEGQTQSESFISSGSTETDGLAFALSGDVALPSTRPQS
                     GNVVLLDRFGTNVITWLDPSDARVIAQLAVGTGFESNPSDYLEVSNREAFITRWGDNA
                     QPGQQENDRGGDVLFVDLESHEISGSLELPRQYGSDAEEYPARPAGMTRIGDTVWISL
                     SRLSSDFANMGEALLVGVSISKHEVQQVLSVHGLKD"
BASE COUNT         1900 a         3318 c         3111 g         1849 t
ORIGIN      
        1 gctatcacgt gtacgagggc gcggcaatgg acctcgcgcg taagttgccc caagcctgtt
       61 acgggccgaa gcaggcccta tcgacgggtc gcaagcgcgc cgcgggcctg aactcgccga
      121 gcgcacaggc gtgggcgctg cgggacgcgt tcgacgggct gctcggcgtg gtggcccgcc
      181 gcttgaagca cggcaaagac tagcgcagca actcgaacgc gatttggcgt gctcgccgaa
      241 ggtttcgacg agcggcacga gaaaggccta ctccgaaacc caagtcgtag ccggagatgg
      301 ccagcgcgtg cccctccggc atcgcgccgt agagcgccat gcaagtgctc agcagggagg
      361 ctggcccacc cacgtgcgaa accggagcct gctcgcgccg tgtctgatcg gacaattcgg
      421 ccggtgtggc cggcaagcgc gtgagctgca cccccaacac gcttgggtcc agactcgcgt
      481 ccacgaacag tactcgcggc gcgcgccgca gctcgaggct gagctcgggc aaaagttcga
      541 tctcgagttg gacttcgacg tgtggaagcc gagcccgctg cacgcaccgc gcgagatagt
      601 gtcctagccc gtcgtcacgc cgcagcgggt taccgtagcc gatgactaac caacgcaagt
      661 tctctgagct caggagcggc tccctgtctt attgatcgga gccgctccgg ctccgctcta
      721 ttgtctcatc ggagccgctc cggctccgat gggccaagac gcggccatcg gggtcgagga
      781 gcgtgacttc gagcggcatt tggccaatgg cgtgggtcga gcagctcaaa caagggtcga
      841 aggagcggat gccggcctcc aagcggttga gtatgccttc tgggatctcc gggccacgga
      901 tgtagtgttc cgcaatttgg agcaccgttc ggttcatggc ggtgtggttt tgcccagtcg
      961 cgatcagcag agtcacgcgc tgtacgaccc cattctcgtc caccttgtaa tggtgaaaga
     1021 gtgtgccgcg aggggcttcg ctcacgccca cgccttccaa acaattgatt tcagcatgcg
     1081 cgcggatgcg ccccgatagc aattcgggat cgtcgacgag ccgttgaatg agctcgactg
     1141 cgcacagcat ctcgatcagc cgcgcgtagt gataaaggaa cgacgaagtc tggggagggg
     1201 cgaagcgttg gcgatattcg attagttcgc gatcggccag cgtggtcccc attcgctcgc
     1261 agacgttgag gcgcgcgagc ggccccaccc ggtacgcgcc gtgggggtag ccgagcggcc
     1321 gatagaatgc cggcttcatg tacgatccga agtccaccgc ctctgcgatg aactcttggt
     1381 agttcgccgc gctcagtccc tcggccacga cgcgatcgct cgagtccacg aacttgagct
     1441 ttcccccgta gtgctcccag ctgccgtcgg gagccacgag cgacatgaac aagctcggga
     1501 atcggccgaa attggcgatc tcatcaccaa agccgtctag taaacgcttg aaccgttcga
     1561 gggcaccttg cgtcgtctgc aacgcctccg gcagaagctc gcgcagctcg tcgcgtgccg
     1621 cctccgagag cggagcgcgc acaccgcctg gcaccgccca tgacggatga atcttgcgcc
     1681 cacccagcag ctcgatcacg cgctgtccga actttctcaa ccggatgccg ccgcgtgcgt
     1741 actcgggatc ctgcgcagcc aatccgaata gatttcgccg ttcgggcggc gactccatgc
     1801 cgaggagcag atccggcgag ctcaaatgga agtagctcag cgcgtgggac tgcacgattt
     1861 gggcaaggtt catcagccgg cggagcttgt cggccgcggc ggggacgttg acggccaaga
     1921 tggcgtccgt cgccttggct gaagcgagca aatggctcac cggacagatg ccgcaaactc
     1981 gcgaagtgat gccaggcatt tccgcgagcg ggcgaccttc gcagagtttc tcgaacccgc
     2041 gaaactccgc gacgtggaat cgggcgtcac tgaccttgcc cgagtcgtcg agcatgatgc
     2101 tgattttggc gtgtccttca atccgagtga ccggatcgat cacgatgcgc gtcgtcacga
     2161 gaacctcgct tccttcaacc gaacgaaagg tcacgcccgg acaagccggg agccccgcct
     2221 gccagcaact gctcgatgag cttacgaatc cgagatgcct cgggtgggca tccggtgagg
     2281 aagccatcga ccttcaccac ctcgtgcacg ggcaacacgt ggtcgagtaa cgccgggatg
     2341 atgccgggct cacccgggat gcgcggctgg cctgcgacat cctgcaaata agcccgatcc
     2401 agaaccggaa gtgcatgccc gagcgggttg cgcaaagcgg tgacgttgcc cgtcaccgca
     2461 caatcgccaa atgccacgag cagccgcgtg cgttctcgaa ccttggcgat tatttccaga
     2521 tgctccacgt tggccacggc cccttccacc aacgcgatat cgacgccctc ggggtaatcc
     2581 ttgcgatcga tcacggggct gtagacgatg tctgcgaatt gggtgagctc gagcaggaac
     2641 tcgtccaagt ccaagaagga catatgacat cccgagcaac cgcccagcca gagcgtggcc
     2701 aacttcacac gttccattgc ttcttctccc gcgcgttgac caagaaggag atcacgcccc
     2761 gatccttgcg cagctcaccc acgctggtgc cgcgtgggaa gatcgcaccc gtcgggcacg
     2821 cctcgacaca cttgccgcac gacgtgcaag tgctgctctc ggcccagggc tgtttcaaat
     2881 cggtgatgac gcgggcgttg accccgcgac ccgaaatatc ccaggtgtgt gcgccttcga
     2941 cctcgtcaca aactcgcacg caacgtgtac acattacgca gcggttgtgg tcgattccga
     3001 aacgagcgtg gctcacgtcc acctcgcagt tcgggtgcag gtactcgtag cgaacatggt
     3061 cgatgccgag cacggacgcc aagttctgca gctcgcagcc gccgttggcc acgcacaccg
     3121 agcaaacgtg attccgctcg gcgaacagca actcgacgat catctttcga tacgatgtca
     3181 gtcgctcact ttgagtccga acccgcatgc cctcggcgac gtgcgtgacg cacgcgggcg
     3241 cgagcttgga tgagccttcg atttccacca gacacagtcg acaggcaccg gcgttactgg
     3301 tgcccggcaa gtgacacagg gtggggatgt agatgcccgc ttcgcgcgcg gcttccagca
     3361 atgtaacgtc ttcgcgcgcg ccgatgggtt taccatcgat gctcaaggtt ttaacggcca
     3421 tgcgcggctc cttctggtga ggaagctttg gcttgttgcg tggtgtggcc ctcggcggag
     3481 tcgttcaggc gcctggtgta ctcttcgcgg aaatagcgca gcgtgctctt caccgggttc
     3541 ggcgccgact ggcccaaccc gcacaggctc gcggctttga ccatgtccgc cagctcttcg
     3601 agcttcacga ggtcggagct ccgcgcggag ccacggccga tgcgctgtaa caggtgatgc
     3661 atctggacgg taccggcccg gcaaggaatg cacttgccgc acgattcatc catggagaac
     3721 tccatgaaat atcgcgcgat gtcgaccata tcggtcgtct cgtccatcac gatcatgccg
     3781 cccgacccca tgatcgagcc gagtgctgcc aacgagtcgt agtctaccgg cgtgtcgagg
     3841 tgagcctcgg ggatacagcc tcccgaaggt ccgccggttt gtacggcctt gatccgcccg
     3901 ccattcggag tacccccgcc cattacctcg acgatttcgc gcagggtggt gcccatgggc
     3961 acttcgagca agcccgtgtt cttcactttg ccggccagag cgaacacctt ggtgcctttg
     4021 ctgttggcgg ttccgatcgc cgaaaaccag gccgagccct tgagtacgat cggcggcacg
     4081 ttcgcgaacg tctcgacgtt gttgatcacg gtcggatagc cccacaaacc gtgctcggcc
     4141 ggataaggag gccgcggcac gggcgtacca cgccgccctt cgatcgacgc catcagcgcc
     4201 gtttcctcgc cgcacacgaa ggcgcccgcg ccgatgcgca gatcgatctt gaagtcgaag
     4261 ggtgattcca gaatctggct gccgagcaag cccaagcgct tggcctgttt gatcgccgtc
     4321 tccaggcgcc gaatcgcgag cgggtactcg ccgcgcacgt atacgtaacc ttgcgatgcc
     4381 cccaccgcgt agcccgcgat aaccatgccc tcgagcacgc ggtgcggatc actctccagc
     4441 acgctgcgat ccatgaatgc ccccggatcg ccttcgtccg cgttgcacac tacgaatcgc
     4501 ttgctggcaa cctgcttggc caccgtgtcc catttcaggc cggtcgggta gcccgcaccg
     4561 cctcgcccgc gcaagccgct ctcgatcatc gttgcgacga cctgacgggg ctccatttcc
     4621 cggagcgcgt gccagagcgc ccgatagccc ccgcgcgcga tgtacgactc gatccgatcg
     4681 ggttcgacga tgccgctgtt ttccagcacg atcggttgct ggcgcgtgaa gaaggcgtgc
     4741 tgcgtatcgg ccttgggcag ctcgttgctc ggcccgctca gcgcggagac gatggcctcc
     4801 gcgtgttcag gcttgacctt ctgatacagc ggcccgcccg aagagacctc gaccaaaggc
     4861 ccttcacaac acagtcgctg acagccgact tcgagcacat ccacctcttg ctcgcgtcca
     4921 gcttgcttga tggcgccttc tagcgcctct cgcaccgcgc gggagcccga cgacaagcag
     4981 cccgcggcgc cgcagcaccg caaatgcaaa cgccccgaac cgctgcgctc cttttcggag
     5041 atggcgtcta gatcagcctg gtccaccttg cctccacttc ttcagggccg cgattgcaag
     5101 ctcggcagtc tggtgagcgt gcacttcacc atcgtacacg gcgattggcg cgagcccgca
     5161 ggccccaagg caacgcgccg tagacaggga aacctcgccg tccgcgcgcg tcttgcccgc
     5221 cactacgccg agttcctgtt cagagacgct caagatggcg tctccgcctt tcacgtagca
     5281 agcagtgccg gtgcacacca cgcaggtgtg ccgcgccgga gccgcgaacg agaacagatg
     5341 ataaaacgtg gcgacgccgt aaacgcggct cgggggcagc ttcagcgcct tggcgacgtg
     5401 cagcagcacg tccttctgga gccgcccgaa cagctcctgc gccgcgtgca agacctcgat
     5461 cagagagtct ggctgaaagc gctggcgctt catgaaagag tccagcatct cgaagcgggt
     5521 gtccttctta tttaccgctt cggacgggga ctccgggagc cgagcggggc cacccgaagg
     5581 gctggtggca gcggaacgag tgcgcatcat ggattcaact ccgcgctgga gagccaatcg
     5641 aaccaagccg acatgccttc gccggtttcg gcagacacct tcatgaaccg aggccgggcc
     5701 atgactcgct cgagcgccga ctggattacc ccgacatcca cccggggcag gtgcgggagc
     5761 agatcgatct tggtaatcac cacgagatcc gcgttccgga acatgactgg gtatttcagc
     5821 ggcttatctt caccctcggt gaccgacaac gcgacgacgt tgcgagcctg gccgagatcg
     5881 taaatcgctg ggcaaactag atttccgacg ttctcgataa acaacatcga gaattctcga
     5941 ttcaactcat gaagagccgt gtgcaccaga tcggcatcca agtgacaagc cgaccccgtc
     6001 gtgatggcac atgacggaat tcccgccgcc gtcaggcgct gcgcgtcgtg gtccgtcgcc
     6061 agatctccgc tcatggcggc gagtgtgcgg cggccggcga gcgcgcgccc ggtggcctcg
     6121 agcagggcgg tcttgcccga tccgggtgaa cccatcaagt tgacgcaaaa cacgccgcgt
     6181 cgcgcgaagt ggctccgatt gtgctcggct gcggtctgat tccgcagcag caaagactcg
     6241 tgaatgtcga ctttcacgag ttttgcatca ccacaaccac aaacttcaca cattctctac
     6301 ctcgctctac tcacgtggcg cttcactcac tggcacttcg agctcgatgc gctccaggct
     6361 caactcatca cccgatctca atcgggctgg taagtcgcag cgcggacaca acgaaccaca
     6421 cggatcttct gattcgaact cgacatcgca acgcacgcac tggaaagtcg ccgttacaat
     6481 ctcgatttgg agctcggctc cggcacatac ggtattttcc cgcagacacc gaaacgccgt
     6541 ttcgaacaac gcgggttcta cgccagacac tcgcccgatg cgcaccgcga cgcggctcac
     6601 ggacttggca cggtgacgac gcgcctcatc cgcgatacgg agcagcaggc tctcgaccac
     6661 cgaatattca tgcaagcggc ggcacctcct gcgacggaac atgtgcaacg aaagacacga
     6721 accgggagac ctcctgctgg gtcgcttcga ccagcgtgct aggccttgct cgttccagac
     6781 ctgctcgatg gtctagaccc tcttaccgac tcctaccgtt cgcgaaggaa cgcagcaaga
     6841 gtccgtcgca aggccagcaa gccgaactgc gctcgatcaa tgagagatca gggcgtccgc
     6901 gagcaccgtg caagcgtcac gtccgagggc gcccgcctga tcctctgctc agctatcgcg
     6961 gagcgccggt gcgcctgggt gtgctttggc ggaaaccgct tgccgcgcgt tgacgcatcc
     7021 gctatcagcc ctctcgttgt cgccgccagc ccctgtgggc tggctcaagg gaatccggtg
     7081 tcaatccgga gctgccccgc agcggtattg ggaacgaact ccgcctcgaa gcactggctc
     7141 ggacgagagc tgggaagcgg cggaaagtag atggctaaca gcccgtcggt ttactgaacg
     7201 agtggttcat caaacggcgg ttctagcagc cgagccctga gcccgaagac ctggcgtcaa
     7261 cggcgtggcc accgggctgc gcctgagtcg cttctctcgc gggagggagc gaattcaacc
     7321 tccgcttcga gccatctcca cgcctgttgg cgctcgctcg gcctcttttc ggccgggcac
     7381 gggagctcga accactcgtg ggtagctcga tggcggagct cgggtctcct atgccgcttc
     7441 cgttcgtcta cgcacagcct gcaaccgcag caccggtgga aatagtcgtg cagggcgaac
     7501 gtgcgcaacc gctggcggca gctcgcgagc ggcaggtctc gggctcggtg gtcgagaggg
     7561 agcgactaac ccagcccggg gtgaccgtgg cacaagtgct gcgagatgaa ccgggcgctc
     7621 aaaccacgga gttgggcgcg tacggaacct ggtcgagcat ttcactgcgc ggtgcgcccg
     7681 gcgctcagac gccggtctac ctgggtgcgt tgcggctcaa cgacgatctg ggtggggccg
     7741 cgaatctgag tgacgttccg ttattcatcg tgcaacgggc agagctctac cgcagccacg
     7801 cgccgggctt cgtcgaacaa cttggcatcg gcggggcctt ggtgttcgag ccgaagcgtc
     7861 ccgaacagaa ccaactcgcc gccaatatca cgatcgggag ttacggttac gcgactgccc
     7921 aaggcttcgc cgccgcccga tccggcgaac ggcgcatgct tgccgcagtc gagttgaccc
     7981 acgccgacaa cgattacgag tttcccaacg ggaacgggac gctcttccag cgcggggacg
     8041 atcgcgtcac caggctcacc aacgccgact tgcggcaaag taacgtgtgg ctaagcgcca
     8101 gtgaccaagt cggacgagcg gccctgcgtt tgacctatca ccgcgcgaat cgggaacaag
     8161 gcgcgctgaa gctggcggtc acgcaggcca agcaggcgcg cgtagccacc aaccgcgacg
     8221 ccgtggtgct cgagagcgca gtgccgctcg attcgtggcg cggcaagctg ggcgtctcca
     8281 cacaggcgat gatcgcggat accgaattgt tcgacccgct gtcggagctg agcctggccg
     8341 cgacgcgaac tctgacccgt gctcaacgcg tagaacaggc gggttttctt cagcatgaca
     8401 tcctctcggg ttggcaatcc accgagcgca ttacgctcgg cttcgaagga ctgcagcgct
     8461 tcgaggagcg agattcgcgc gactccgaac gttcagccgc cacgcgctcg cgtgcacgct
     8521 tctcgacctc tcaccaaatt cgattcggcg aacacctgag cgcgacccta ctcggctcac
     8581 tcgccagcta tacgacgtcc ggggcccagc atcagggcgc cggggcatcg gattcggcgc
     8641 gaacgagccc cacgctaacc aacgcgccgc agctcgaacc cgagggtcgg gtcgggctcg
     8701 cctatcgcgg gggcacgcgc gaactgttcg tcaacgcggg acattatcgg cgctccgcca
     8761 ccttggccga gttgtttggc gcgtcgttgt tggtacgcgg caacccagag ttgcactcgg
     8821 agcgagggac tacgatcgag ctcggagcgc gccaacaatg gctcgatgca ctttgtgagc
     8881 gccgggcctg gctcgatctg ggttcgttcg cgcgcttcag tcacgacttg attgcctatg
     8941 acactacggc tcaaggttat ctgttgccca agaaccgcaa gcgaactcgg gccctgggtg
     9001 cggagctgtc gctgggcgct cgaccgctcg actttctcac ggcggagctc gatgtgagcc
     9061 tggttgacgc gcgcgacgtg agcccaggcg gcttgaagaa tgagctcctg ccgttcgtga
     9121 gccgcgccac ggtgtcgtcg ttcctcgcct tcgagcagag gttcgacgcc gattggttgc
     9181 agcgcgcccg gctggggctg cgtgccaact acaaatcgag tcgttttgcg aaccaagccg
     9241 gcgctggcgt catccccgag cagctggtgt tcgacttgga ggcggctgcc actggcctgt
     9301 tcgacgccct aaccgcgcgc ttacgcgtcg cagacgtctt cgatgccacg cgcttcgacg
     9361 tggtcggttt tccgctgcct cgccgcagct tctatttcgc cctggagtct accttatgaa
     9421 aaacctcgca atcattcgat ccgtgctggc gctctgcgct gctttgggcg gcgcattcgc
     9481 atgcaacgtc gagaatacga ctcatgacac cggaggcgtc ggcaccaagt ccgatcccga
     9541 gagttgcccc gccggcgtca gcatcgtgct cagcgactac atggcgacgc agatcgcgct
     9601 ttcggatctg gaggggcaaa cccagtccga gtccttcatc tcttcgggca gcacggagac
     9661 cgacggattg gcgtttgcgt tgtcgggcga cgtcgcttta ccgagtacgc gaccgcaatc
     9721 gggcaacgtc gtgctgctcg atcgctttgg caccaacgtc attacctggt tggatcccag
     9781 cgacgcgcgc gtgatcgctc agctcgcagt gggcaccggt ttcgagtcga acccgtccga
     9841 ctacttggag gtatcgaacc gcgaagcatt cataacccgc tggggcgaca acgcacagcc
     9901 aggacaacag gagaatgacc gcggcggaga tgtgctattc gtggatctcg agagccacga
     9961 gatcagcggc tcgctggagc tgccccgcca gtacggcagc gacgccgagg agtacccggc
    10021 gcgccccgcg ggcatgaccc gcatcggaga tacggtttgg atctcgctca gccgactgtc
    10081 ttccgacttc gccaacatgg gcgaggcgct gctggttgga gtctccatct ccaaacacga
    10141 ggttcagcaa gtgctcagcg ttcacggctt gaaggatt
//
LOCUS       DASTKU010000086         3893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1577531, whole genome shotgun sequence.
ACCESSION   DASTKU010000086 DASTKU010000000
VERSION     DASTKU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577531"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFQ61_02945"
     CDS             <1..375
                     /locus_tag="VFQ61_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953431.1"
                     /translation="TKEYPPEGADAYGAIPDDLVDFISFSVVPAPGPPGVGGTSDPIE
                     PRSQADDLRPEDAKQFDALDEHNRRAAPSPHPAQLEPALPTEPALPTEPALPTDPTQL
                     TEPSSQDDVAESADKRDVQPIA"
     gene            415..1161
                     /gene="tatC"
                     /locus_tag="VFQ61_02950"
     CDS             415..1161
                     /gene="tatC"
                     /locus_tag="VFQ61_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET9953432.1"
                     /translation="MTLWEHLEELRSRIIRMMLSFIGGAALAWIFRERLLAWLTEPFV
                     QAWSSLPQGGKASLNFASPAALFISYVRLSAVAGLVFALPLILYQVWAFIAPGLYSRE
                     KRFALPFVVSSCVLFAAGGWFGWRFAFPAAFHYLLGFAGEVGQLEVKPTVMVNEYIEF
                     VTHMLLAFGFAAELPILVFFLSVAGLVTHRQLIKFFRYFVVLAFVISAVLTPPDPLSQ
                     LMLAVPLCALYGISILIAMLFGRKRESADT"
     gene            complement(1172..1600)
                     /locus_tag="VFQ61_02955"
     CDS             complement(1172..1600)
                     /locus_tag="VFQ61_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET9953433.1"
                     /translation="MRIVVIAQGKLKERGLREVADDYLTRIRKHVRCEEIEVRKASEL
                     AARVPKDATVVALEVNGDRVTSSELSQRLSLWLSRDKGSVAFLIGGSEGLPEELSRSA
                     RVRLSLSTLTLPHRLARLLLLEQIYRGLSIARGEPYARED"
     gene            complement(1613..2068)
                     /gene="rsfS"
                     /locus_tag="VFQ61_02960"
     CDS             complement(1613..2068)
                     /gene="rsfS"
                     /locus_tag="VFQ61_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET9953434.1"
                     /translation="MKPTSTLRPLVSATNKDGKDRAQLRAEGPSPDAKRIALLAAEAG
                     LDKKASEVEIIDVRGKVDYADYLVLMTGHSDRHVSAIAEGVDHSLGTKNFHSISVEGL
                     QRGEWVLIDFVDVVVHVFQERTRSLYDLDGLWMDARRVELPSAPKPETA"
     gene            complement(2149..3396)
                     /locus_tag="VFQ61_02965"
     CDS             complement(2149..3396)
                     /locus_tag="VFQ61_02965"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET9953435.1"
                     /translation="MSSVREQAEKARAAARVLRSQGRAAKDAALRAIANKLRAHAGPI
                     RERNLVDIENARKNGMSSAMLDRLRMDEPVVEATARAVEYVASLRDPVGSRSSMQRLE
                     NGLLAGRERVPLGVIAMVYESRPNVTIDAGVLCLKSGNAVLLRGGKEAAATNQLLGEL
                     VRDAVRESGLPEAAIQVVPPTDRDGIRELLSLTGLVDLAIPRGGEGLIRFVTENARVP
                     VIQHYKGVCHLFLDAGCDVEKAVRVAVNAKAQRPATCNALECLLVHAGDATRVLPPVA
                     RALLDKGVELRGCPRTLELVPEAKRAQDSDFGFEFSELILAVRVVDDLDGALDHIAQY
                     GSNHSEAICTENLGHAERFLREVDASCVLVNASTRFNDGGQLGLGAEIGISTSKLHAY
                     GPMGLESLTTEKWVVLGDGQVRS"
BASE COUNT          670 a         1280 c         1162 g          781 t
ORIGIN      
        1 accaaagagt acccccccga gggtgccgat gcttacggcg cgattcccga cgacttggtc
       61 gatttcatct cattcagcgt agtccccgcg ccgggtccgc ccggcgtcgg tggaacctcg
      121 gatccgatcg agcctaggtc gcaagcggac gatctgagac cagaagatgc gaagcaattc
      181 gacgctctgg acgaacataa ccgtcgagct gcgccgagtc cgcatccggc gcagctcgag
      241 cccgctctgc ccaccgagcc cgctctgccg accgagcccg ctctgccgac agacccgact
      301 caattgaccg agccgagtag tcaggacgat gtagctgaat ccgctgataa gcgagatgtt
      361 cagccgattg cgtgatccca tatgagcgcc agcgcgaatc ccgaagaaca tacgatgacg
      421 ttgtgggagc acctcgagga gctccgctcg cgcatcatcc gcatgatgtt gtctttcatc
      481 ggaggagcgg cgctggcctg gatcttccgg gaaaggctgc tggcctggct gacggagcca
      541 ttcgtgcaag cttggtcgag tcttccgcag ggcggcaaag cgtcgctgaa cttcgcctct
      601 ccagcagccc tcttcatctc gtacgtgagg ttgtcggcgg tggcgggact ggtcttcgcg
      661 ctgccgctca ttctgtatca ggtctgggcc ttcattgcgc cgggcttgta ctcgcgtgag
      721 aagcgcttcg ccttgccgtt cgtggtgtcc tcgtgcgtgt tgttcgcggc cggtggctgg
      781 ttcgggtggc gtttcgcatt cccggcggcg ttccattact tgctcggctt cgccggcgaa
      841 gtgggccagc tcgaagtgaa gccaacggtg atggtgaacg agtacatcga gttcgtcact
      901 cacatgctgc tcgccttcgg cttcgccgcc gagctgccga ttttggtgtt cttcctttcg
      961 gtcgcgggcc tggtgacgca ccgacagctg atcaagttct tccgctactt cgtagttctg
     1021 gcgttcgtga tctccgccgt gctcacgccg cctgatcccc tgagccagct gatgctcgcg
     1081 gtgccgcttt gcgcgttata tggaatctcg attttgatcg cgatgctgtt tggtcgaaag
     1141 cgcgagagcg cggatacgtg aacccgcgct ctcaatcctc acgagcgtaa ggctcccccc
     1201 gcgcgatgct caaaccacgg tagatctgct cgagcaggag caacctcgcg aggcgatgcg
     1261 gcagcgtgag cgtagagagg ctcagccgca cgcgcgccga gcgtgacaac tcttccggca
     1321 aaccctccga gcccccgatc aggaacgcta cggagccttt atcccgagac aaccagagcg
     1381 aaagccgctg ggagagctcg ctgctggtga cccggtctcc gttcacttcc aaggccacta
     1441 ccgtcgcgtc cttcggcacg cgcgctgcga gctccgaggc cttccttacc tcgatctctt
     1501 cgcagcgaac atgcttgcgg atgcgcgtca gatagtcgtc cgctacttca cgcaagcccc
     1561 gctctttgag cttgccctgc gcaatgacta cgatgcgcaa taactacgat cctcaagccg
     1621 tctcgggttt gggcgcgctc gggagctcga cgcgacgggc atccatccac aagccgtcga
     1681 ggtcgtacag ggaccgggtg cgctcctgga aaacgtggac aaccacgtcc acgaagtcga
     1741 tgagcaccca ctcgccacgc tgcaatccct cgacggagat gctgtgaaag ttcttcgttc
     1801 cgagcgaatg atcgacgccc tccgcgatcg cggagacgtg ccgatcgctg tgtcccgtca
     1861 tcaggaccag gtagtccgcg tagtcgactt tgcctcgcac atcgatgatc tcgacttccg
     1921 aggccttctt gtcgagcccc gcttcggccg ctaagagggc gatgcgcttc gcatcgggcg
     1981 aagggccctc ggcacggagc tgtgcgcgat ctttgccgtc tttattcgtt gcggaaacca
     2041 ggggacgcaa tgtgcttgtt ggcttcaact tttcgattct ccgtattttc ttcctgtcgt
     2101 ggtggtgtgc tgcatagctc gtaccagtac ttcgccggca aacttgcctt agctgcggac
     2161 ctggccgtcg cctagcacca cccatttttc cgtagtcaga ctttccaggc ccatggggcc
     2221 ataggcatga agcttggacg tcgagattcc aatttccgcg ccgagtccga gttggccccc
     2281 atcattgaat cgggtcgatg cgttcacgag aacacagctt gcgtccactt cgcgcaggaa
     2341 gcgctccgca tgcccgagat tctccgtgca aatcgcctca ctgtggttcg aaccatactg
     2401 ggcgatgtga tcgagcgcgc cgtccagatc atccacgacg cggaccgcta ggatgagctc
     2461 ggagaactcg aatccgaagt ccgagtcctg cgctcgcttc gcctcgggca ccagctccag
     2521 cgtcctgggg cagccacgca gctccactcc tttgtcgagc agcgctcgcg ccacgggagg
     2581 taacacccgt gttgcgtcgc ccgcatgcac gagcaggcat tctagagcgt tacaagtcgc
     2641 gggccgttgc gccttggcat tcacggccac tcgcaccgcc ttttcaacgt cgcatccggc
     2701 gtccaggaac aggtgacaga cgcccttgta gtgctggatt acgggtaccc gcgcgttttc
     2761 agtaacgaag cgaatcaacc cctcaccacc acgcgggatg gccagatcca ccaacccggt
     2821 gaggctcagg agctcgcgaa tgccatcgcg atcggtgggc ggcacgactt gaatggccgc
     2881 ctcaggtaag cccgactcac gtacggcgtc tcgaacgagc tcgccaagca gttgattggt
     2941 ggccgcagct tccttgccac cccgcaacaa aactgcgtta cccgacttga ggcacaagac
     3001 accggcgtcg atcgtgacgt tagggcgcga ctcgtagacc attgcgatga caccgagcgg
     3061 cacgcgctct cgtcccgcga gcagcccgtt ttcgaggcgc tgcatcgagc tgcggctacc
     3121 gaccggatca cgtagggagg cgacatactc gacggctcgt gccgtagcct cgaccaccgg
     3181 ctcgtccatc cggaggcggt cgagcatcgc cgagctcatg ccgttcttac gcgcgttctc
     3241 gatgtctacg aggttacgct cacggatcgg ccccgcgtgc gctcgcaact tgttcgcgat
     3301 cgctcggagc gctgcatctt tggcggctcg accctgactg cgcagcacac gcgcggccgc
     3361 tcgcgctttc tctgcctgtt ctcgcacgct actcatgtcg gtagcgcttg gtctagcaga
     3421 tctcagtgcg gccccccacc ccccggaccg gtctgcttgt gcgcggggcc cgcggggtgc
     3481 tcggggatga ccgccggcgg gctacccgca cgaggagcgc ttgccccggc tgcggcggct
     3541 gcgccctctt gcttagcgct cgaatctgtt tgggttggtt ccccgacagt ttcgcggctc
     3601 gggggcatcc cgtcaacggg tgcgcctggc tcaggctcct gcgccgcatt cggcggagcc
     3661 gaagaagggt tccaagggac gaccttcgct ggctcgtatt cgggttctgg taaacctgca
     3721 ggaggccgac gaggcgcgcc gccgcaagac accaatgccc acccgacgag gacccaagaa
     3781 accgcctcga gcgcacgagc cttcacgcta agcgcgccta cgggaaggcg aaaagtcacg
     3841 ttagtcagag cacgcatcgt cacagcgaca ctccatcggg ctccgccggc ggc
//
LOCUS       DASTKU010000087        21970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_685271, whole genome shotgun sequence.
ACCESSION   DASTKU010000087 DASTKU010000000
VERSION     DASTKU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 21970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21970
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685271"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1083
                     /gene="bioB"
                     /locus_tag="VFQ61_02970"
     CDS             55..1083
                     /gene="bioB"
                     /locus_tag="VFQ61_02970"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044968914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HET9953436.1"
                     /translation="MSADGSVDSPLNDSSLRHDFSLEEVRALHDLPLFELLDRARRTH
                     LQFHPQGEVQLCTLLSVKTGGCPEDCGYCSQSSHHSTGVKGEPLMKLPDVVAAAQRAK
                     EQGATRFCMGAAWREVKDGPQFERVIDMIKEVKAMGLEACVTLGMLNEEQAARLKAAG
                     LDAYNHNLDTSREHYKSIISTRRFDDRLATLKNVRKAGITVCSGGIIGMGESIDDRCR
                     LLIELSSLKPHPESVPVNALSQIEGTPLANLPPVEPTELVRMIAVARLMMPKSKVRLS
                     AGRSQLSREAQLLCLYAGANSIFYGDRLLTTSNPSESEDAELIRLAGLRPEAPKSCGA
                     PEGSETCC"
     gene            complement(1188..2096)
                     /locus_tag="VFQ61_02975"
     CDS             complement(1188..2096)
                     /locus_tag="VFQ61_02975"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET9953437.1"
                     /translation="MSKALRYSFVLAGAYLFCTPSALAFCRTNTCDPSKGDTCTLDSA
                     GCRTGGHDLAWVSSCVTYAVQEDGSKANQVSASVFENILADAFATWKAADCGDGKHPS
                     INVESLGLVSCNKPEYNSDQGNANVFMFDDEDWMTTGSGDALGLTTVWFDRKTGQIYD
                     ADVEVNGTGRLTHGSPQDGADLQSIVTHEIGHFLGLDHANTQSAVMYATYNPGRDNLR
                     LLRDDDVKGICAIYPPSRQAASSECEPRHGFASTCAVPKEDEGCSFSVPRKSSNTPLS
                     RGAFPVLFALAGLVGWARGRRRRVTR"
     gene            complement(2093..2671)
                     /locus_tag="VFQ61_02980"
     CDS             complement(2093..2671)
                     /locus_tag="VFQ61_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953438.1"
                     /translation="MKNLTRRGALGMLAAGVGGLFQARLAHATLLRGMTVPTLARSSR
                     HIVAVTPLAAEAHWEVLGGRRRIVTDTRVRIDDVLAQEDPADSEMMVRTLGGTIGDLG
                     AIVFGEAELTPQQPCVLFLMQKGDGPPRVTGLAQGHYPLRLDPKRSDAKVRLFGSPRL
                     PELLGDEELAVKRLVGLQLGQARQLVREALKQ"
     gene            3009..5684
                     /gene="glnD"
                     /locus_tag="VFQ61_02985"
     CDS             3009..5684
                     /gene="glnD"
                     /locus_tag="VFQ61_02985"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HET9953439.1"
                     /translation="MAESTGTHVQVLSSPSLPAELKSYLSNHRGSVEAKVRSRDEGAG
                     LAAAERLSKAYDGLFCSLFKAVECTLTKQGNWTSVAMAAVGSYGRSALALYSDLDVRL
                     VTAGRPEKVRHIAEAMLYPLWDVGLSIGHQVVSPSDLLELAKNDLPTATTLLDWRVVA
                     GERATTERMLSRAFEGLFGVSNIGKFLERLTDRAEERARRFGDSLYLLEPDVKNGIGG
                     LRDLDLMHWAARARWRVENLEELVRRGALLPREWQRIDAAARFAWRIRNLLHLHAGRR
                     SDRLSFDLQEVLAEDLGYGSGGPAVEQFMSEYYRHARVLWQGREMLLQRAEPPPKRRP
                     RESPIGRGLKLTNDQVSIENPLDLETDPALSLRLFDEAVRRDLPVYPFARDSVARATA
                     SPSFGEKLRGSEEAARLFARLLTTVQRTRLRSGSVLKELHDVGLLVAMVPEFAPVVGR
                     VHHDVYHVYTVDVHSVAAVDRLRELCRGELASEHPLASRLAAEISHPSVLFFAALLHD
                     IGKDIGGRAHDERGARMAGEILARLGLSERDTREVQHLVLKHLRMYHVATRRDLDDPK
                     TIESFCEEVHGSEGLRELYLLTLCDVSTTSPTALTNWKARVLEELYRAAERHLAGESE
                     RGSERLEQVKSAVLGLCPERGEREFLQHFLDAMPERYLYANEPDDIVRHSRFARQAQM
                     RQVNVTVMTTLDPYVELAFIADDRPGLLAMITATLTAARFKVISAQVYSWVDAFGRTR
                     ALDLFWVRGSTVDAVTGSPARLERDFSRLLSHELSPPELVTGGMRTSRWSDRPTPKVQ
                     TDVNVDNRCATRDTVIEVTTRDQLGLLFWLSNTLQSLGLTISLAKINTEGTQVADVFY
                     VTDENGQKITDPTAIERIKGRILATVAHLENGANA"
     gene            5681..6721
                     /locus_tag="VFQ61_02990"
     CDS             5681..6721
                     /locus_tag="VFQ61_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9953440.1"
                     /translation="MTIECVGGQRRNGRRLGSIWALAALLCWGCGGSQTPAANASDPP
                     LEDAPSAKPAASVPASSAKVQQGIDAIQKQDFKAAQAVLTEARAEAPKDPQAAFYLGV
                     ALEGAGSPDLAEKEYRAALELDPKLVDATANLSALLVDAGKTREALPIIEQGLKAAPK
                     HPELLVNRAICLEAEGRDDVALQAYAAALEVRADAPDLHLAYAKLLAKAGKNPEAIEQ
                     LRAASAAEAPEVLSAIADLFGKLKAPADCVALMDRVLKAQPSPYFFVRRGACRHDASD
                     ESGARADFEAALKLEPRFAPAHYYLGMHFVTKQDKKAATQHLTQAIQIDGEQGSVGKR
                     AKKALQDLKGAR"
     gene            complement(6845..7309)
                     /locus_tag="VFQ61_02995"
     CDS             complement(6845..7309)
                     /locus_tag="VFQ61_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953441.1"
                     /translation="MRLSSKLRWWLLLTVVFADGCFAEDDGGGSCLNPQPEPPCNGPV
                     ATGGTTGIPEAGRPPAMGGSLSGAGAGNSSAGGGNSASGGLSAGFGGSSTGGHPDSGA
                     AGSNAPAGGDGGAPEGGAAGADPIGGAPNAGAGGDAGAAGEPATGGALDFRG"
     gene            complement(7590..8537)
                     /locus_tag="VFQ61_03000"
     CDS             complement(7590..8537)
                     /locus_tag="VFQ61_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET9953442.1"
                     /translation="MSDVTRKPPPPRGLGRGLDALLPVRPAPGDANAGMHVNYGEGNV
                     FSCPLDKIVPNRGQPRQHFDADKLEELAQSIREHGLIEPLVVRRRPNSDQFELIAGER
                     RWRAAQRANLREALVVVRDVSARDAYELALIENVQREDLDPIEFAEALDRLIREHGHT
                     QETLATRLGKDRSTIANALRLLRLPGQIREKVVQGELSEGHARALLGAPDDQTLTELA
                     GKVIRGRLSVRKTEELVRKAKSHENEDSSPAASGKPPQAPGKGSPPPGKSPSVRDLET
                     RLERRLGTKIDVRDHEGKGEIVIKYADWDELDRILDIIL"
     gene            complement(8534..9301)
                     /locus_tag="VFQ61_03005"
     CDS             complement(8534..9301)
                     /locus_tag="VFQ61_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9953443.1"
                     /translation="MRKKPDIIAIANQKGGVGKTTTAVNLAASLAAAERRTLLLDLDP
                     QANASSGVGILPRSAERTIYDALIGTCPLADVIQKTAIELLDVVPATQDLTAVEYELF
                     DEQRGTHLREVLKQDVVKNYEFVILDSPPSLGVLTLNVLSAADRVIVPLQPEYYALEG
                     ITYLMATIDRVKSSLNPRLTVEGIVLTMWDPRNKLAHEVAAQVRKHFRVFEAIIPRNV
                     RLSEAPSHGVPALLYDVSSKGAQGYLSLARELIEVPA"
     gene            9452..9667
                     /locus_tag="VFQ61_03010"
     CDS             9452..9667
                     /locus_tag="VFQ61_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953444.1"
                     /translation="MSRPQTPPEVLRQALRLALASAEVRDAGAEQHKARILERGFWRA
                     PNTGTAACISLLERRALRVSGLLDRGE"
     gene            9674..9859
                     /locus_tag="VFQ61_03015"
     CDS             9674..9859
                     /locus_tag="VFQ61_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET9953445.1"
                     /translation="MARLPKGYTNFADFEREHIRPEMRIGWSADDELENSGDGLDFDM
                     DPFEAALWAAESEDSAD"
     gene            10015..11316
                     /gene="tig"
                     /locus_tag="VFQ61_03020"
     CDS             10015..11316
                     /gene="tig"
                     /locus_tag="VFQ61_03020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET9953446.1"
                     /translation="MTGKVRPPIMQVTLQRLSPVLVEFDVQVAADRVKNELDRAYSEV
                     ARSARVPGFRPGKAPRRVLAHYYGARIQADVAQKLVDETFPKAVEDHQLQPVNRPAIE
                     TQKLSESTDFTYKARVEVLPKIESVEYEGLEAKRPKVAVADAEIDQEIDRLRRAHATL
                     QAPSEARPTKAGDLLTLDLEVEVDGKRVSELAAQDLNLEIGSQQVLPELSTGLEGHSQ
                     GETVPVQIQMPAQHPSPQLRDKLATFHATIKEVKERVLPEVDDEFAKDVGDFESLAAL
                     REDIRQRLEKQSKETSDNQLAERLVVALVGKNPIPVPPSLVEQQMRITSQEILARARA
                     YGQEANEVGAELRQQIQTDSEVKVRAGLLMAEIAKKEQIQIDNEAIEKGIQELAEQTG
                     KNPAKLRVEYREPKKREMLIGMLLENKVLDIIEAKAKIEDE"
     gene            11446..12108
                     /locus_tag="VFQ61_03025"
     CDS             11446..12108
                     /locus_tag="VFQ61_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET9953447.1"
                     /translation="MAKRPETRAEAVRLLESISEPTRANIIYPHVVETTHRGERTWDI
                     FSRLLKDRIVFLGSAVDDDVANIIIAQFLFLESEDPDKDVQLYINSPGGVVSAGLAIY
                     DTMQYVRCPVSTICIGQAASMGAVLLGAGQKGRRFALPNSRIMIHQPLGGARGQATDI
                     EIQAREIRYLKDVITDILVRSTGKPRDEIAKDIERDFYMGPGQAKEYGLIDEVFEPKK
                     KG"
     gene            12357..13610
                     /gene="clpX"
                     /locus_tag="VFQ61_03030"
     CDS             12357..13610
                     /gene="clpX"
                     /locus_tag="VFQ61_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET9953448.1"
                     /translation="MERRRDESNGNLCCSFCGKSQKEVKKLIAGPTVYICDECIALCN
                     DIIAEEVEKDEPAAGSDPMPKPSEIRAILDDYVVGQERAKKVLSVAVYNHYKRIDTKV
                     STDDVELGKSNILLLGPTGSGKTLLAQTLAKILKVPFAIADATTLTEAGYVGEDVENI
                     IVQLLQNADHDVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIIEGTI
                     ANVPPKGGRKHPQQDFLQVDTSNILFICGGAFVGLDQIIQRRLGQSRLGFGADVKSKN
                     EMKLGELLSNVQPEDLLKFGLIPEFIGRLPVIATLHELNEDALIDILTKPKNSLVKQF
                     QKLLEMDGVKLKFTKGALQAIARKALARESGARGLRAILEEAMLEIMYDVPSKQGIKE
                     VVVNEEAIASGENPLIVYHKEAGVG"
     gene            13632..16118
                     /gene="lon"
                     /locus_tag="VFQ61_03035"
     CDS             13632..16118
                     /gene="lon"
                     /locus_tag="VFQ61_03035"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET9953449.1"
                     /translation="MFFRNDPSDKSGQPRRSLPALPLRDIIVFPHMVSQLFVGREKSI
                     AALDDAMAQTKEIFLAAQKSAKTNDPAPDDIYAVGTVGVIVQLLRLADGTVKVLVEGQ
                     RRARVRRFLDTEGFFRVEVEEVHEQAQNDVEVEALIRSVQSTFEVYVKLNKKVQPEVL
                     MSVQSIDDASRLSDTIAANLPTIKLADRQGLLEMEDAKKRLERLYELMQAEIEILQVE
                     KKIRSRVKKQMEKTQKEYYLNEQMQAIQKELGGGERDEFKSEIQEIEDKLKNKKLSEE
                     AQQKVKKELKKLKMMHPTSAEATVVRNYIDWILSLPWTDKTEERFDIAEAERILNEDH
                     YGLKKCKERIVEYLAVQALTKHLKGPILCFVGPPGVGKTSIARSIARATGRKFVRLSL
                     GGVRDEAEIRGHRRTYIGALPGKIIQNLKKVGSNNPLFLLDEIDKMSTDFRGDPAAAL
                     LEVLDPEQNSTFNDHYLDLDYDLSDVMFITTANTLGGIPLPLQDRMEIIQLTSYTEFE
                     KLNIAEKYLIPRQKKECGLEQVQLEINENAIRTVIHHYTREAGVRSLERELASICRKA
                     ALRVVKEGKDQKIEIRGADVPKYLGVPRYRLGRKDESDMVGLTHGLSVSDHGGDILDC
                     EVSVVPGKGKLVITGLLEKGMEESAQAAMSYIRSRAGALRIDPEFYQKVDVHVHFPDF
                     VRKDGPSAGVTMATSIASALTRVPIRSDIAMTGEITLRGRVMPIGGLKEKLLAAHRNL
                     IATVLIPKENRKDLKEVPRRVLRGLRIVLVEHMDDVLREALRFEDPDALFGPRREVLE
                     YRNGELVQDEGALAARSLGTGSAEEQPGLS"
     gene            complement(16199..17035)
                     /gene="larE"
                     /locus_tag="VFQ61_03040"
     CDS             complement(16199..17035)
                     /gene="larE"
                     /locus_tag="VFQ61_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HET9953450.1"
                     /translation="MNPRTNVDKLEHLSRLIRELGSLLVCYSGGIDSALVLAVATREL
                     GSQAIGMTAVSPSLAEAEHEDAARVARQLGARHYFVHSHEIERPGYVQNGPDRCFHCK
                     TELYTIAQSKASEWQLQYIANGTNTDDLGDYRPGLEAARQAGVKSPLVDAGLNKADVR
                     ELSRHLELPIWDKPAAACLSSRIPYGSAVTPERLGQIGGFEAELRQLGLRQVRVRWHD
                     AIARIEVPVSELGQLLNEPTREAILQAGKRHGFTYVTLDLAGYRQGSHNELLQGRSLR
                     LI"
     gene            17293..18300
                     /locus_tag="VFQ61_03045"
     CDS             17293..18300
                     /locus_tag="VFQ61_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953451.1"
                     /translation="MTSPTSATPSSVHRSASLLEVEAGLIKAGLGAKAGLISKAVRSP
                     NASGNPSATLISSNTAVAGRLIEAPGAVLSAPLARTENPNLAKPTRIEPTGVLPFGIT
                     ELDGVLPEGGLMKGGVVELAAGEGVPATSLALAVCRSMQQFRAHQGQAMSWAAFVDPS
                     ESLHAPGVLACGVHLEQLLVVRPPLEALARSVLRLAKSSAFELVVVDLLGTAGAILKL
                     NLDPWVRWVRRLAIELEGSARSVLLVTDLSAHRTLPLPVAQRLELKRPKVDRLWLRVA
                     KDRNGHVTAPRSITWRGVASGSPTTASDRASPLQSNLAYGPTAPVQARPTPEELHVAS
                     G"
     gene            18611..20200
                     /locus_tag="VFQ61_03050"
     CDS             18611..20200
                     /locus_tag="VFQ61_03050"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HET9953452.1"
                     /translation="MEAVNGEAQRLGVRRGQTVAEARAITARLDIQQVSRSAIEHGLA
                     RVAEIALAFGAVVSFEMPDTVWVDITGAAHLLGGETALLAELVSRVREAGHLVRGAVS
                     VGPLLARALARFGTLSEEGFIVVPSAHTARSLAPLPLKALNLTGAGLNEEVEGWLIRL
                     GVLNVGELLALPRAALATRLGKSADSVLTLAQGVDATPLTPYAPPRTISEQAQFEEAL
                     TITEPLLFVLRGLCARVSARLAGRGEAAFRLALELQHDPGIARFRNTQREVRLPMTLP
                     KPIWNARELYRLLATKLERYELAAPCVGIRLEASELVGAMPRQLELGFGGSHDAVSAL
                     AELPLVIAELAADVGEDRVGVLRAVDAHRPELTSELRPAFADNADSTQVSKSRSRRAD
                     TTRGPIRSNVASKPRHSTGLHSGSAPHAEALPSPQVASLPVKHGETAEPVHRVTRLLK
                     EPVPLHTALRTGATLVLGHRMYTIESLRFAQRLHAVEWWSQSISRDYVRVVLKGSDGV
                     LEGLVYVDRDNGKRYFQGVAD"
     gene            20551..21783
                     /locus_tag="VFQ61_03055"
     CDS             20551..21783
                     /locus_tag="VFQ61_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9953453.1"
                     /translation="MAIAGGCSQTPEVPTSGLAGDFGSPLTSGGNTHAASGGHAGTAG
                     TFAKGGQPMIGASGESSAAGSSAYDFDTCARATLEAEPSKTNLLFVIDKSGSMNCNPP
                     DGDIERAARCARYPVQEDKTRPSKWQVTQTALMDAISGLRGRPNLKVGLSLFPKSTAC
                     DSAREPDVTLADLDDAQLRRLESTLSNVAASGETPLAAATIRGYGHLADQLKAHTLEG
                     SSLVILLTDGTETCAPNLLDDLLTIHAPNARLFQIRTFVIGVPGSEGARSFLSNLAFI
                     GGTSVSGCSHPLLPNDAGDCHLDSTRSLNLANDLKQALERVTSDRAIACTLDVPRNPT
                     GGAVDFTKVNVTFSPLGGEATNIPYEPSATCDASGRGWQYSPDFRRITLCGAACSTVQ
                     AQPGTLDVVLGCPTEVIR"
BASE COUNT         4265 a         6610 c         7000 g         4095 t
ORIGIN      
        1 cacctgacag gcgctctgga cgcctcgacg ggttcccgct aaagctgccc aaccatgtct
       61 gctgacggct ccgtcgattc tccgctgaat gattcttcgc tgcgccacga tttcagcttg
      121 gaggaggttc gtgccttaca cgacctgccg ctgttcgagc tgttggaccg cgctcgccga
      181 acccatttgc agttccatcc gcagggcgag gtgcagctct gcacactcct gagcgtaaag
      241 accggtgggt gtcccgagga ttgcgggtac tgctctcaat catctcatca ttcgacgggt
      301 gtcaaagggg agcccctaat gaagctcccc gatgtggtgg ccgccgccca acgcgcgaaa
      361 gagcagggcg caacccggtt ctgcatgggt gcggcgtggc gtgaagtgaa ggatggcccc
      421 cagttcgagc gcgtgatcga catgatcaag gaagtgaaag ccatggggct agaggcctgc
      481 gtgactctcg gcatgctgaa cgaagagcag gcagcgcgct tgaaggccgc ggggctcgat
      541 gcgtacaacc acaacctgga cacttcgcgc gagcactaca agtcgatcat ctcgacgcgt
      601 cgcttcgacg ataggttggc gaccctcaag aacgtgcgca aggcaggtat cacggtttgc
      661 tccgggggca tcattgggat gggggaaagc atcgatgatc gctgtcggtt actgatcgag
      721 ctctcgagtc tcaaaccgca tccggagagc gttcccgtca acgccctttc tcagatcgag
      781 ggcactccgc tcgcgaactt gccgccggtc gaaccgaccg agctcgtacg catgatcgcc
      841 gtggcgcgct tgatgatgcc gaaatccaag gtgcgtctca gtgccgggcg ttcgcagctc
      901 agtcgtgagg cacaactgct gtgcctgtat gccggcgcca acagcatctt ctacggagat
      961 cggctgctca cgacctcgaa cccgagcgaa tcggaagacg cggagctgat tcggctcgca
     1021 gggctgcgcc ccgaggcgcc aaagtcgtgt ggggcgcccg aaggttccga gacgtgctgc
     1081 tgagccggcg ccgagtcgtg ggaacgtaag gttggagagg cgtcgagctg ttctcgtcgc
     1141 ctctgtgccc gtgccgcggc gtgacggtta gccgatcgcg gtcaggctca acgtgttaca
     1201 cgcctgcgtc taccgcgggc ccatccgacc agtcccgcca gcgcgaacaa tacggggaaa
     1261 gctccgcgtg aaagcggggt attggagctc ttgcggggaa cgctgaagct gcagccctca
     1321 tcttctttgg ggacggcgca agtgctcgcg aagccgtgac gcggttcgca ctcggagctc
     1381 gcggcttgac gactcggcgg atatatcgcg caaatcccct tcacgtcgtc gtcgcgcagg
     1441 aggcgaaggt tgtctcggcc ggggttgtac gtcgcgtaca tcaccgcgct ctgagtgttc
     1501 gcgtgatcca ggccgaggaa gtggccaatc tcgtgcgtca cgatggactg caaatccgcg
     1561 ccgtcttgcg gtgacccatg cgtgaggcgg ccagtgccat tgacttcgac atccgcgtcg
     1621 taaatttggc cggttttgcg gtcgaaccag acggtcgtga gccccaacgc atctccgctg
     1681 cccgtcgtca tccagtcttc gtcgtcgaac atgaagacgt tggcgttgcc ctgatcgctg
     1741 ttgtactcgg gcttgttgca gctcacgagt cccaagcttt ccacgttaat cgaggggtgc
     1801 ttgccgtcac cgcaatctgc ggccttccag gtcgcaaaag cgtcagcgag gatgttctcg
     1861 aacacactcg ccgaaacttg gttggccttt gaaccgtcct cttggactgc gtaggtgacg
     1921 cagctactga cccacgccag atcgtgaccg cccgttcggc agcccgcaga atccaacgtg
     1981 caagtgtctc ctttgctcgg atcacaggtg ttcgtgcggc aaaacgcgag cgcgctggga
     2041 gtgcagaaca agtaagcgcc cgcgagaacg aacgagtagc gcaaagcctt gctcattgct
     2101 tcaacgcctc tcgcacgagc tgacgtgctt ggccgagctg cagcccaacg aggcgcttca
     2161 cggccaactc ttcgtccccg agcagctccg gcaagcgagg gctcccgaat aggcgcacct
     2221 tggcgtccga acgcttggga tccagacgca acggataatg gccttgagcg aggccggtca
     2281 cgcgaggggg cccatcaccc ttctgcatga ggaacaagac gcacggctgc tgcggcgtaa
     2341 gctccgcttc cccaaacacg atggctccga gatcaccaat ggtgcccccc aaggtacgta
     2401 ccatcatctc ggaatcagct ggatcctctt gcgcgaggac gtcatcgatt cggacgcgcg
     2461 tgtccgtaac gatccggcgg cgaccgccca acacttccca gtgggcctct gccgcgagcg
     2521 gcgtgacggc aacgatgtgc cgactcgatc gcgccaaggt aggtaccgtc atgccccgga
     2581 ggagggtggc gtgcgccagc ctcgcttgga acaggccacc aaccccggcc gccaacattc
     2641 ccaacgcacc tcttcgagtc aggttcttca tcccgcgtct cataactata ctttcctgag
     2701 tcaccgtcac aaggtcgaac cagaaaaatg gagtgtcagc cggttcggta gggacacgcc
     2761 agggcagggg ccaacgtacg gctcgcatgt ctacgaaggc gtccatggtg gagggttctc
     2821 ggcgtaggag cggcgttcgc agaaccggtt cccggacgaa atgcaacggc ccgtccgccc
     2881 agctgctata tagagctccc gacccatagt ataccgcgcg cttccgctgt cccacccctc
     2941 gagacctttg agccccatga cgttgtcgtc cacaaaacgc gcgctgctgg cgccgtccgt
     3001 tccacaacat ggctgaaagc actggcacgc acgttcaagt tctttcgtcg ccgtcgttac
     3061 cggcggagct gaagagctac ctcagcaacc atcgggggtc cgtcgaggcc aaagtgcgca
     3121 gccgcgacga aggtgccggg ctagctgccg ccgagcgcct ctccaaggct tatgacggtc
     3181 tgttttgttc gttgttcaaa gccgtcgagt gcacgctaac gaagcagggg aactggacca
     3241 gcgtcgccat ggctgccgtt ggcagctacg ggcgcagcgc gttggcgttg tacagcgatc
     3301 tcgacgtgcg gctggtgaca gcagggcggc ccgagaaggt gcggcacatc gcggaagcca
     3361 tgctctatcc gctgtgggac gtggggctca gtattgggca tcaagtggtc agcccgtcgg
     3421 acttgctgga gcttgccaag aatgacttac ccacggcgac gacgttgctc gattggcgcg
     3481 tggttgcggg tgagcgtgcg accaccgagc gcatgctcag ccgcgcattc gagggtctgt
     3541 tcggcgtctc caacattggc aagttcttgg agcggctaac ggatcgcgcg gaggagcgcg
     3601 ccaggcgctt cggcgattct ttgtatttgc tcgaacccga tgtgaagaac ggcatcggcg
     3661 gcttgcgtga cctggacctc atgcactggg ccgcgcgcgc gcgctggcgg gtcgagaact
     3721 tggaggaatt agtgcgccgg ggcgcgctgc tgccgcgtga gtggcagcgc atcgacgcgg
     3781 cggcgcgctt cgcttggcgc atccgcaacc tgttgcacct gcacgcagga cgacgctccg
     3841 atcgtttgag cttcgatttg caggaggtgc tcgccgagga tctcggctac ggcagcggtg
     3901 gtcccgcggt ggagcagttc atgagcgaat actatcgcca tgcacgcgtg ctctggcagg
     3961 ggcgggagat gctcttgcag cgcgccgagc cgccacccaa gcgtcggccg cgagagagcc
     4021 ccatcggccg aggtttgaag ctgaccaacg atcaggtgtc gatcgaaaac ccactcgatc
     4081 tcgagacgga tccggccctg tccctgcgct tgttcgatga ggcggtacgc cgcgatctgc
     4141 ccgtttatcc gttcgcgcgt gactccgtgg cccgtgctac cgcatcacct tcgttcggcg
     4201 agaaactgcg ggggagtgag gaggccgcgc gtctgttcgc gcgcttgctg actaccgtgc
     4261 aacgaacccg tttgcggagc gggtcggtgt tgaaagagct gcacgatgtg ggcctgctgg
     4321 tcgcgatggt gccggagttc gcgccggtcg tgggccgggt gcatcacgac gtgtatcacg
     4381 tgtatacggt ggacgtgcac tcggtggccg ccgtagatcg gctgcgcgag ttgtgccgcg
     4441 gtgagctggc aagcgagcat ccgctagcct cgcggctcgc ggccgaaatt tcccatccga
     4501 gcgtgttgtt cttcgcggcg ctgctgcacg atatcggaaa ggatatcgga gggcgcgccc
     4561 acgacgaacg tggcgcccgc atggcgggcg aaatcttggc ccggctaggg ctgagcgagc
     4621 gtgacacacg cgaggttcag cacctggtgc tcaagcacct ccgcatgtac cacgtggcga
     4681 cccggcgcga cctggacgat cccaagacca tcgagtcttt ctgcgaagag gttcatggca
     4741 gcgaaggcct acgtgagctg tacctgctga ccctgtgtga cgtctccacg actagcccta
     4801 ccgcgctaac caactggaag gcgcgcgtgc tggaggagct gtaccgcgcg gcggagcggc
     4861 acttggcagg tgaatccgag cgcggcagcg agcgtttgga gcaggtgaag agcgcggtcc
     4921 tagggctctg ccccgagcgc ggcgagcgcg aatttttgca gcacttcctc gacgcgatgc
     4981 cggagcgcta cctatacgcc aacgagccgg acgacatcgt tcgccactcg cgcttcgcgc
     5041 ggcaagcaca gatgcggcag gtcaacgtca cggtcatgac cacgctcgac ccgtacgtag
     5101 aactcgcgtt cattgctgac gatcgaccgg gcctcttggc catgattacg gcgaccctca
     5161 ccgccgcccg cttcaaggtc attagcgcgc aggtctattc gtgggtggat gcgtttggta
     5221 gaacgcgcgc actggatctt ttctgggtcc gcggcagcac cgtcgacgcg gtcaccggct
     5281 cgccggcacg actggagcgg gatttcagcc gcttgttgag ccacgagttg agcccacccg
     5341 aactcgtgac gggtggaatg cggacctcgc gctggtccga tcgcccaacc cccaaggtcc
     5401 agaccgacgt caacgtagac aaccggtgcg cgacgcgcga tacggtgatc gaggtgacga
     5461 cgcgcgatca attgggtctg ctgttttggc tctcgaatac gctgcagtcg ctcgggctga
     5521 cgatttcgct cgcgaagatc aacaccgagg gaactcaagt cgcggacgtc ttttacgtga
     5581 ccgacgagaa cgggcagaag atcactgacc caacggccat cgaacgcatc aagggccgca
     5641 ttttggccac cgtcgcccat ctcgagaacg gagcgaacgc atgacgatcg agtgtgtggg
     5701 cggtcagcgc cggaacggcc gccggttggg ttcgatttgg gcattggcag cgttgctatg
     5761 ctggggatgc ggcggaagtc agactccggc cgcgaacgcc tcggacccac cgctcgaaga
     5821 cgcgccgagt gccaagcccg ccgcttctgt ccctgcatcg agtgccaagg tgcagcaggg
     5881 catcgacgcc attcagaagc aggatttcaa agccgcccaa gccgttctga ccgaagcgcg
     5941 cgcggaagcc cccaaagacc cccaggcggc cttctatctg ggagtggcgc tcgaaggtgc
     6001 gggcagcccg gatttggctg aaaaagagta tcgagccgcg ctcgagttgg atcccaaatt
     6061 ggtggatgcg acggcgaatc tgtcggcgct gctggtggac gcgggcaaaa ctcgggaagc
     6121 gttgccaatc atcgaacaag gtttgaaggc tgccccgaag cacccggagc tgctcgtcaa
     6181 tcgcgcgatc tgcctcgaag cagagggccg cgatgatgtc gccctccagg cctacgcggc
     6241 cgcactcgaa gtacgtgcgg atgcacccga tctgcacctc gcctacgcga agctactggc
     6301 caaagcaggt aagaatccag aggcgattga gcagttgcgg gcggcgtccg cagcggaagc
     6361 accggaggtg ctctcggcga ttgccgacct gttcggcaaa ttgaaggcac cagccgactg
     6421 cgtggcgctg atggatcgtg tgctgaaggc ccaacccagc ccatacttct tcgttcgacg
     6481 gggggcttgt cggcacgatg cttcggatga atcaggcgct cgcgccgact tcgaagctgc
     6541 cctcaagttg gagcctcgct tcgctccagc ccactattac ttgggcatgc acttcgtgac
     6601 taaacaggac aagaaagcgg ccactcagca tctcacgcaa gccatccaaa tcgacggtga
     6661 gcagggcagc gtgggcaagc gagccaagaa ggcgctccag gatctgaagg gcgcgcgata
     6721 agtccttcgt cgctgagcag agctgggctg ggctgagggg gtccgcgttt gaacgaggtc
     6781 ttgcgggcaa ggcctcgtcg aaagccgtcg ggcttgtcga ctccgctccg ccaattctgc
     6841 gctgtcaacc gcggaaatct agcgcaccgc cggttgccgg ctcacccgcg gcgccagcat
     6901 ccccgcctgc acccgcgttc ggtgcgccgc cgatcgggtc cgcgcccgcg gcgccaccct
     6961 ctggcgcacc accatcgccg ccagctggcg cattcgagcc cgcggctccc gaatctggat
     7021 gaccgccggt cgaggaaccg ccgaaccctg cgctgagccc gccggatgcg ctattgccac
     7081 cgcccgcgga gctgttgcct gcgccggcgc cggacagact gccgcccatc gcaggcggcc
     7141 tgcctgcttc gggaataccc gtggttccgc cggtcgctac gggcccattg cacggcggtt
     7201 ccggctgcgg gttgagacag ctccctccac catcgtcctc ggcaaagcag ccatccgcga
     7261 agaccacggt cagcagcaac caccaccgga gttttgaaga gagcctcatc ccttagtggc
     7321 tcaggatacc gcacatgctc cgcccgtcca gcgcgcaacc aatactgatc gatctcatcg
     7381 gcggatcgga acggattcgc ggacacccgt tcttgccgac cccgccgcgc ttgcggtcct
     7441 ccagagcagg ttctggccca gcgtctcgag agcgtctcgg cacgtcatgt caacgacatg
     7501 aaggatcgcg ctgatgctcc agcaccaaaa cttttcgcct tgggtcggag cgaacgggcc
     7561 cgccacgcga aggcgcgtcc gagctcacgc taaaggatga tatcgagaat gcggtcgagc
     7621 tcgtcccagt ctgcgtactt gatcacgatt tcgcctttgc cctcgtgatc gcggacgtcg
     7681 atcttggtcc caaggcgccg ctccaatcgg gtctctaggt cccgcacact cggagacttc
     7741 ccaggtgggg gcgagccctt gcctggtgct tgcggcggct ttccgcttgc cgccgggctg
     7801 ctgtcttcgt tctcgtggct tttcgccttg cggaccagct cttcggtctt gcgcacgctc
     7861 agtcggcccc ggatcacttt ccccgcgagc tcggtcaacg tctggtcgtc gggcgccccg
     7921 agcaacgcgc gcgcgtgccc ctccgagagc tcaccctgca cgactttctc gcgaatttgg
     7981 cctggcaagc gcagcaaacg caaagcgttg gcgatcgtcg aacgatcctt accgaggcgc
     8041 gtcgcgagcg tctcttgggt gtgcccgtgc tcgcggatca gtcgatctag cgcctcggca
     8101 aactcaatcg gatcgaggtc ttcgcgctgg acgttctcga tcaacgcgag ctcgtacgca
     8161 tcgcgtgcag agacgtccct caccaccacg agcgcttcgc gcaagttggc gcgctgcgcc
     8221 gcacgccagc gtcgttcacc cgcgatcagc tcgaactgat cggagttggg ccggcgccga
     8281 acgacgagcg gttcgatcaa accatgctca cgaatggatt gcgcgagctc ctcgagctta
     8341 tcggcatcga agtgttgccg cggctgcccg cgattcggca cgatcttgtc gagcgggcag
     8401 gagaagacgt tgccctcccc gtagttgacg tgcatgccgg cgttggcatc tcccggcgcg
     8461 ggtcgcactg ggaggagggc gtccaatccg cgtccgagcc ctcgcggcgg ggggggtttg
     8521 cgcgttacgt cgctcacgct ggaacctcga tcagctcccg cgccaagctc aagtagcctt
     8581 gggctccttt gctgctcacg tcgtaaagca gcgccgggac gccgtgcgag ggtgcttcgg
     8641 acagacgcac gttacgagga atgatggctt cgaaaactcg gaagtgtttg cgcacctggg
     8701 ccgcgacctc gtgcgcgagc ttgtttcgag ggtcccacat cgtcagaacg atgccctcga
     8761 ccgtcagccg cgggttcagc gaagacttca cgcgatcgat cgtcgccatc aagtaagtaa
     8821 tgccctcgag ggcataatac tcgggctgaa gcggcacgat cacgcgatcc gccgccgaca
     8881 gcacattcag cgtgagcacg ccgagcgagg gcggcgaatc caagatcacg aactcgtagt
     8941 tcttgaccac gtcttgcttc agcacctcac gcaagtgcgt tccgcgctgc tcgtcgaaca
     9001 gctcgtactc cactgcggtt agatcctggg tggcgggcac cacgtccaac agctcgattg
     9061 cggtcttctg aatcacatcg gcgagagggc aggtcccaat cagagcgtcg taaatcgtgc
     9121 gctcggcgct gcgcggcaag atgcccacac cgctcgaagc attggcttgt ggatccaagt
     9181 ccagcagcag ggtccgtcgc tcagccgcag cgaggctcgc cgctaagttg accgccgtag
     9241 tcgtctttcc cacaccgccc ttttgattgg cgatggcgat gatgtctggc ttcttgcgca
     9301 tttcaggccg gcccgagctg gttcgtccca ttatcacgtg gcgggccccg gcgcgcggtc
     9361 cgagcgtcct agcgacaggg atcgccagaa ttttggaaac ggcgcgaagc tcgcgtactg
     9421 tgtcgttaga tgtaggtgaa ggtaatcgaa agtgagtcga cctcaaaccc ctccagaagt
     9481 tttgcgtcag gccctgcggc tcgcattggc gtcagccgag gtgcgcgacg ccggcgctga
     9541 acaacacaaa gcgcgcattc tcgagcgggg tttttggcgt gctccgaaca ccggcaccgc
     9601 tgcctgcatc agcctgctcg agcgccgcgc gttgcgcgtc tcgggcctct tagaccgagg
     9661 agagtgacgc gacatggcac ggcttcccaa aggctacacg aacttcgcgg acttcgagcg
     9721 tgagcacatc cggcccgaaa tgcgcatcgg ctggagcgcg gacgatgaac tcgaaaactc
     9781 cggagatggc ttggacttcg acatggaccc attcgaggcc gctttgtggg ccgccgagag
     9841 cgaagattcg gctgactagg aacgcctagc cgctcacgcc tcccaacaga cttgcagaaa
     9901 cccataagac cccgccgggc tgcctcccgg cggggtcgac ctttttccgg ggaacgcggc
     9961 tggttacggg gtccgagttg aggatgcgag cggcggcctc ttgacctagg caccgtgacg
    10021 ggtaaggtcc gacccccgat catgcaggtc acgcttcaga ggctcagtcc cgtcctcgtc
    10081 gaattcgacg ttcaggttgc cgccgaccgc gtcaagaacg aactcgacag ggcatattca
    10141 gaggtcgctc gctcggcgag ggtgcccggg ttccgccccg gcaaagcgcc gcgtcgtgtg
    10201 ctcgctcact attacggcgc gcggattcaa gccgacgtag cgcaaaagct cgtcgacgaa
    10261 actttcccga aggcggtcga agatcatcag ctgcagccgg tgaatcgtcc tgccatcgaa
    10321 acgcagaagc tctccgagtc cacggatttc acgtacaagg cgcgcgtcga ggtgctgccg
    10381 aagatcgagt ccgtcgagta cgaagggctg gaagccaagc gcccgaaagt tgcggtagcc
    10441 gatgccgaga tcgatcagga gatcgatcgc ttgcggcgcg ctcacgcgac gttgcaggcg
    10501 ccgtccgaag ctcgcccgac caaggctggg gacctgctca cgctggatct cgaggtcgaa
    10561 gtggatggca agcgcgtgtc agagctggcg gctcaagact tgaacctgga gatcggttcg
    10621 cagcaagtgt tgcccgagct cagcaccggc ctcgaagggc actcgcaagg ggagaccgtg
    10681 ccggttcaaa ttcagatgcc ggcgcagcac ccgagcccgc aattgcggga caaactcgcg
    10741 acgttccacg ccacgatcaa agaggtgaag gagcgcgtgc tgcccgaggt ggacgatgag
    10801 ttcgcgaaag acgtcggcga cttcgaatcg ctagcggctt tgcgcgaaga catccgtcaa
    10861 cgcctcgaaa agcagtcgaa agaaaccagc gacaaccagc tggcggagcg tctggtcgtg
    10921 gccctggtcg gcaagaaccc gatcccggtg ccgccttcgc tcgtcgaaca gcagatgcgg
    10981 atcacctccc aagaaatctt ggcccgcgcc cgggcttacg ggcaggaagc caacgaagtc
    11041 ggagcggagc ttcgtcagca gattcagacg gacagcgagg tcaaggtgcg tgcgggtctg
    11101 ctgatggccg agatcgccaa gaaagagcag atccagatcg acaacgaggc catcgaaaag
    11161 ggcattcagg aactcgccga acagacgggt aagaacccgg ccaaactgcg cgtagagtac
    11221 cgagagccca agaagcgcga gatgctcatc ggcatgttgc tcgagaacaa ggtgctggac
    11281 atcatcgagg ccaaggccaa gatagaagac gagtagctcg cccagagggg ctttgcagcg
    11341 tttccagttc ttgaaccctg ttcgcgaagc cggaaaaccc gtccggaaag cgttgtctgg
    11401 ccccaacccc gacccagtat tctgcagcga ggaggactat ccaaaatggc aaagagaccc
    11461 gaaacccgcg ctgaagccgt gcggttgctc gagagcatca gcgagcccac ccgtgccaac
    11521 atcatctacc cgcacgtggt ggaaaccacg catcgaggcg agcggacctg ggacatcttc
    11581 agccgtcttc tgaaagatcg gatcgtcttc ctcggcagcg ctgtcgatga cgacgttgcg
    11641 aacatcatca tcgcccagtt cttgtttttg gagagcgagg atccagacaa ggacgtccag
    11701 ctctatatca actccccggg cggcgtcgtc tccgcgggtc tcgcgatcta cgacaccatg
    11761 caatacgtcc gctgcccggt cagcaccatc tgcatcgggc aggccgccag catgggcgcc
    11821 gtgctgctcg gagccggtca gaaagggcgg cgtttcgcgc tgcccaattc tcggatcatg
    11881 atccaccagc cgctcggtgg tgcgcgtggc caagcgacgg atatcgagat ccaggcgcgc
    11941 gaaattcgtt acttgaagga tgtcattacg gacattctgg ttcgttcgac gggcaaacca
    12001 cgcgacgaaa tcgccaagga catcgagcgc gacttctaca tgggaccggg tcaggccaag
    12061 gagtacgggc tgatcgacga ggtcttcgag ccgaagaaga agggctgact cactgccttt
    12121 cgttctgttt ccaaggcagc ttctcgggaa gcatgatgtg ctggtcgggc gcccagtgtg
    12181 tgagcgcccg accctggcca ccagcatcca caatgccccg ggttacctcg caccccggat
    12241 ggccgtacca aaaaacatcc cctgactgaa gcggcaggta ccgaagcctt gccctgccgg
    12301 acagagcggc ttacattttt cctacatttt aggcttccga ccccagaggg caacacgtgg
    12361 agagacggcg cgacgaatcg aacggcaacc tttgctgctc cttctgcgga aagtcgcaga
    12421 aggaggtcaa gaagctcatt gccgggccga cggtttacat ctgcgacgag tgcattgcgc
    12481 tctgcaacga catcattgcg gaggaagtcg agaaggacga gcccgccgcg ggttccgatc
    12541 ccatgccgaa gccatccgag atccgcgcga tcttggacga ctacgtcgtg ggtcaggagc
    12601 gcgcgaagaa ggtgctctcc gtcgcagtct acaaccatta caaacgcatc gacaccaagg
    12661 tttctaccga cgacgtggag ctcggcaaga gtaacatctt gctgctcggc cccacgggta
    12721 gcggcaagac gctgctcgcc cagaccctgg cgaagatcct gaaggtgccg ttcgcgatcg
    12781 ccgatgcgac gacgctcacc gaagcgggct acgtcggtga agacgtcgag aacatcatcg
    12841 ttcaactgct ccagaatgct gatcacgacg tggagcgcgc gcagcgcggc atcgtctaca
    12901 tcgacgaaat cgacaagatt agccgcaaga gtgacaaccc ttcgatcacg cgggacgtgt
    12961 ctggtgaagg cgttcagcaa gcgctgctga agatcatcga aggcacgatc gcgaacgttc
    13021 cgcccaaggg tggacgcaag catccgcagc aggatttcct gcaggtagac acgagcaaca
    13081 tcctgttcat ctgcggcggc gcgttcgtgg ggctggatca gatcattcag cgccgtttag
    13141 gtcagtctcg tttgggcttt ggcgcagacg tgaagagcaa gaacgagatg aagctcggcg
    13201 agctcttgtc caacgtgcag ccggaagacc tgctgaagtt cgggctaatc ccggagttca
    13261 tcggccgctt gccggtgatt gccacgttgc acgagctcaa tgaagacgcg ctgatcgata
    13321 tcttgaccaa gccgaagaac agcttggtga agcagtttca gaagctgctc gagatggacg
    13381 gcgtgaagct gaagttcacc aaaggcgcgc tacaagccat cgctcgcaag gctctggcta
    13441 gggagagcgg cgcgcgcggt ctgcgggcca tcctcgaaga ggccatgctc gaaatcatgt
    13501 acgacgtgcc ctcgaagcaa ggcatcaaag aggttgtcgt gaacgaggag gccatcgcca
    13561 gcggcgagaa tccactcatc gtttaccaca aggaagccgg cgtcggctga gccgtgccgt
    13621 ccgaccaagg tatgtttttc aggaacgacc cgagcgacaa gagcgggcag cccaggcgat
    13681 cgctgcccgc gttgccgctg cgtgacatca tcgttttccc gcacatggtc tcgcagctct
    13741 tcgttgggcg cgagaagagc atcgcggcgc tcgatgatgc catggcgcag accaaggaga
    13801 tcttcctcgc tgcgcagaag agcgcgaaga cgaacgaccc cgcaccggac gacatctacg
    13861 cggtgggtac ggtcggggtc atcgttcagc tgctgcgcct agcggacggc actgtcaagg
    13921 tgctggtcga aggtcagcgg cgggcgcgcg tgcgtcgttt tctggacacc gagggctttt
    13981 tccgcgtcga ggtcgaagag gtccacgaac aggcgcaaaa cgacgtcgag gtcgaggcgc
    14041 tgattcgcag cgtgcagagc acgttcgaag tctatgtgaa gctgaacaag aaggttcagc
    14101 ccgaagttct gatgagtgtg cagagcatcg acgatgcgtc gaggctcagc gacacgatcg
    14161 ccgccaatct tcccaccatc aagctcgctg atcgtcaggg cttgctggag atggaggacg
    14221 caaagaagcg tctcgagcgc ctgtacgagc tgatgcaggc cgagatcgag atccttcagg
    14281 tagagaagaa gatccgctcc cgcgtcaaga agcagatgga gaagacgcag aaggagtact
    14341 acctgaatga gcaaatgcag gccattcaga aggagctggg tggcggcgag cgtgacgagt
    14401 tcaagagcga gatccaggaa atcgaggaca agctcaagaa caagaagctc agcgaagagg
    14461 ctcagcagaa ggtcaagaaa gagctgaaga agctcaagat gatgcacccc acgagcgcag
    14521 aagcgaccgt ggtgcgcaac tacatcgatt ggatcttgag cctgccgtgg acggacaaga
    14581 cggaggagcg cttcgacatc gcggaggccg agcgcatcct gaacgaagac cactacggcc
    14641 tgaagaagtg caaagaacgc atcgtcgagt acctggcggt gcaggctctc accaagcact
    14701 tgaaggggcc gatcctttgc ttcgtgggcc cgcccggtgt cggtaagact agcatcgccc
    14761 ggagcatcgc ccgcgccacg ggtcggaagt tcgtgcggct gtcgctcggc ggcgtgcgcg
    14821 acgaggcgga gattcgcggc catcggcgca cgtacatcgg cgcgcttcca ggtaagatca
    14881 tccagaacct gaagaaggtc ggctccaaca acccgttgtt cttgctcgac gagatcgaca
    14941 agatgagcac ggatttccgt ggagatccgg cggccgcgtt gctcgaggtg ctcgatcccg
    15001 agcagaactc gaccttcaac gatcactact tggatctcga ctacgacctg tcggacgtga
    15061 tgttcatcac cacggccaac acgctgggag gcatcccgct cccgctgcag gaccgtatgg
    15121 agatcattca gctcacgagc tacaccgagt tcgagaagct gaacatcgcg gagaagtacc
    15181 tgattccccg ccagaagaaa gagtgcgggc tcgagcaggt ccagctcgag atcaacgaga
    15241 acgcgattcg cacggtgatc caccactaca cgcgcgaagc tggcgtgcga tcgctcgagc
    15301 gcgagctcgc gagcatttgc cgcaaggccg cgctgcgggt cgtcaaagaa ggaaaagatc
    15361 agaagatcga aatccgcggc gcagacgttc cgaagtatct cggcgtgccg cgctaccgct
    15421 tgggccgcaa agacgagtcg gacatggtcg ggctcacgca cggcctcagc gtctcggatc
    15481 acggtggtga catcctcgac tgcgaggtga gcgtggttcc cggcaagggc aagctcgtga
    15541 tcacgggttt gctcgagaag ggcatggagg agagcgctca ggcggcgatg agttacattc
    15601 gctcgcgcgc gggtgcgctc cgcatcgacc cggagtttta ccaaaaggtc gacgtacacg
    15661 tgcacttccc ggatttcgtc cgcaaggacg ggccgagcgc aggcgtaacc atggcgacga
    15721 gcatcgcgag tgctctcaca cgggtcccca ttcgttcgga tattgcgatg accggtgaga
    15781 tcacactgcg gggtcgggta atgccgatcg gtgggctgaa agagaagctg ctggcggctc
    15841 atcgcaactt gatcgcgacg gtgctgattc cgaaagagaa ccggaaggat ctcaaagagg
    15901 tgccgcgccg agtgttgcgc ggtctgcgca tcgtgctcgt cgagcacatg gatgacgtgc
    15961 tgcgagaggc gcttcgtttc gaagatccgg atgctctgtt cgggccgcgt cgtgaggtgc
    16021 tggagtaccg caatggcgag ctggtccagg atgagggggc gctcgcggcc cgctctctgg
    16081 gaaccggctc ggccgaagag cagcctgggc tgtcctgagc tggagcgcgg tccgcgtttc
    16141 agctcagggc tcggctgaac tctgcgttgt aagcgtggag tggagtccgt tgcgacgctt
    16201 agatgagacg cagcgagcgt ccctggagga gttcgttgtg tgagccttgg cggtagcccg
    16261 cgaggtccag cgtcacgtag gtaaagccgt ggcgtttgcc cgcctgcaga atcgcttcgc
    16321 gcgtgggctc gttcaaaagc tgtccgagtt cactgacggg aacctcgatg cgcgcgatgg
    16381 cgtcgtgcca gcgtacgcgc acctgtcgta atcccagttg gcggagttcc gcctcgaatc
    16441 caccgatttg cccgagtcgt tcgggagtga cggcgctccc gtaggggatg cgactcgaca
    16501 ggcatgcggc tgcaggtttg tcccagattg ggagctcgag gtgtcgcgat agctcacgaa
    16561 cgtcggcttt gttcaaccct gcatccacga gtggactttt gacgccagct tgtcgggccg
    16621 cctcgagacc cggtcgatag tctcctagat cgtcggtgtt ggtcccgttg gcgatgtact
    16681 ggagctgcca ctccgaagcc ttgctctggg cgatggtgta gagctcggtc ttgcagtgga
    16741 agcaacgatc cgggccgttc tgaacgtaac cagggcgctc gatttcgtgt gaatgaacga
    16801 agtagtgacg tgctcccagt tgcctggcca cgcgcgctgc gtcttcgtgt tcggcctctg
    16861 ccaaactggg actcacggcc gtcattccga tcgcttggga gccgagctcg cgcgtggcga
    16921 cggcaagcac gagcgcgctg tctatccctc ccgagtagca gaccagcagt gaacccagct
    16981 cacggatgag tcggctgagg tgctcgagtt tgtcgacgtt ggtgcgtggg ttcatgacga
    17041 gcgagctttt acttcaattc gagagcgacg cggtcgccgc gaggtcggga gcgtggtcgg
    17101 gagcgaggtc gtcgtgtgcg gaagtgttgc aggaattttg gtgaatccca gtgtggcgga
    17161 tatagcgcgc agcgttgatc gggcgcgtcg gtttggctgg gacatttttt gtccgtgcga
    17221 gagctaaagc caaggccaag ttgctgagcg atggttgcgc ccaagggtgc ctgatattat
    17281 gttcagtagg tcatgacgtc tccaacttca gctactccgt cgtcggtgca ccgctcggcc
    17341 agtttgcttg aagtggaggc cgggctcatc aaagccgggc tgggcgcgaa agcaggcctc
    17401 atcagcaaag cagtgaggtc gccgaatgct tcgggcaacc cctcggccac cctaatttcg
    17461 tcgaacaccg cggtggctgg aaggctcatc gaggctccgg gagcggtcct gagtgccccg
    17521 ctcgctcgaa ctgaaaaccc aaacctagcc aagccgaccc gaatcgaacc cacgggtgtc
    17581 ctgccgttcg gtattacaga gctggatggg gtcttaccgg aagggggact gatgaagggt
    17641 ggggtcgtgg agctcgccgc tggggaaggc gtgccggcaa catcgttggc tttggcggtg
    17701 tgccgcagca tgcagcagtt ccgtgctcac cagggccaag ccatgtcttg ggcggccttc
    17761 gtggatccga gtgagtcgct gcacgctccg ggggtgttgg cttgcggtgt tcacctcgaa
    17821 caattgctcg tggtcaggcc acctctcgag gcgttggcgc gtagcgtgct gcgactggcc
    17881 aagtcatcgg ctttcgagct ggtcgtcgtg gatctcttgg gcacggcagg ggctattttg
    17941 aagctcaact tggatccttg ggtgcgttgg gtgcgacgct tggcgataga gctcgaaggc
    18001 tcggctcgca gcgtcctgct cgtgacggat ttgtctgcac atcgcacttt gcccctaccg
    18061 gttgctcaac gtttggaact gaagaggccc aaggtcgacc ggctttggct gagagtggcg
    18121 aaagatcgaa acggtcacgt cacggctcct cggtccatta catggcgagg ggttgcttca
    18181 ggctcgccta caacagcaag tgatcgagcc agccctctcc aatcgaatct ggcttacggc
    18241 cccacggcgc cggttcaagc gaggcctaca ccggaggagc tgcatgttgc cagtggctga
    18301 ggagaacccg tctgctgaga gacgtatcgt agctttggtc ctgccgtctc tattgtgcga
    18361 attggcgctt ggtgaagttg ccgttcctac ggccccattc ccccaaaccc aatcaccagc
    18421 tcggtcttcc tcgggagggg ccaagcctcc ggtcagccaa gagcgttcga agagggcggc
    18481 ttcgaggagc tccaagatat tcgccgctcg cgccaaaatg ccgctcgggg tcgtggtagt
    18541 ggagacagcg agcagtgatc gaagaaacgc gagtagcgat aggggcactg aaatcgaggc
    18601 aacgtctcga ttggaagcgg tgaacggcga agctcaacgt ctcggggttc gtcgtgggca
    18661 aacggttgct gaggcacgcg ccatcaccgc gcgactcgat atccagcagg tgagtcgctc
    18721 cgcaatcgaa catgggctag cgcgcgttgc agaaatcgcg ctcgcttttg gcgccgtagt
    18781 gtccttcgaa atgccggaca cggtatgggt cgacatcacg ggcgcagccc atctattggg
    18841 aggagagaca gctcttctgg cggagctcgt cagtcgtgtc agggaagctg gacacttggt
    18901 gcgtggggcc gtttcggtcg gtccgctctt ggctcgagct ctcgcgcgct tcgggacctt
    18961 gtcggaggaa ggcttcatcg tcgttccgag cgcccacacc gcgcgttcac ttgctccttt
    19021 gccactaaag gcactgaact tgaccggagc agggctgaat gaagaagtcg agggttggct
    19081 gatccggttg ggcgtcttga acgtcggtga gcttttggct ttgccgcggg ctgcgttggc
    19141 gacccggctc ggaaagtcag ccgattcagt cttgaccctg gcccaaggtg tggatgccac
    19201 tccgctcacg ccctacgcgc ctccccgtac gatctcggag caagctcagt tcgaagaggc
    19261 attgacgata accgagccgc tgctcttcgt tctgcgtgga ttgtgcgcgc gcgtcagcgc
    19321 gcggctcgcg gggcgagggg aagcggcgtt ccgcttggcg ttggagctgc agcacgatcc
    19381 ggggattgct cggttccgca acacgcagcg tgaggtgcgg ctaccgatga cactaccgaa
    19441 gccgatctgg aacgctcggg agttgtatcg gttgctcgcg acgaagctcg agcgatatga
    19501 attagcggca ccctgtgtcg gcattcgcct ggaagccagc gagctggtgg gggccatgcc
    19561 gcgtcagctc gaacttggat tcggcggctc acacgatgcc gtgagcgcgc tcgcggagct
    19621 gccactcgtg attgctgaat tggcggctga tgtaggggaa gatcgggtcg gggttttacg
    19681 cgccgtagat gcccatcgtc cggagctcac ctcggagttg cgtcccgcat ttgcggacaa
    19741 tgccgattcg actcaggtgt cgaagtcgag atcgcgccga gcagatacga cacgtggacc
    19801 gattcggtcg aacgtcgcga gtaagccgag acattcgaca ggattacatt ctggatctgc
    19861 gccgcacgcc gaggcgttgc cgtcgccaca ggtggcgtca ctaccggtaa agcacggaga
    19921 aacggccgaa ccggtgcatc gggtgactcg gcttctaaag gaacccgtcc ctcttcacac
    19981 cgcacttcga actggcgcta ccctggtgct ggggcaccgc atgtatacga tcgagagttt
    20041 gcgttttgcg caacgcttgc acgccgtaga gtggtggtcc cagtcaatca gtagagacta
    20101 cgtgcgggtc gtgctgaagg gttcggatgg agtactcgaa ggtttggtgt acgtggatcg
    20161 cgacaatgga aaacgatatt ttcagggtgt cgccgattga gcttacctaa acattccggc
    20221 tacgctctcg atgagctgca tcgatggccc tgccatatga gcacgacgac ggcgcaacca
    20281 ccgcgtcagg gatgctcaga cgccgacgca ttccgtaagg cagagcgaga tagagtcagc
    20341 gaagcagagt gagtcacgaa attagtcaga gcgcggtcaa gccataacga aatagaccgt
    20401 tcaactgctc cagagcgaat gcccttcttt gcctggcctg gccccacaac gcctccgagc
    20461 tcgattctca tatcggatgc gcgtagggcc ctgcttcagg tcgggtcgcg acgtggctgg
    20521 cggggctggg ttagcctggt aggtgccggt atggcaatcg ccggcggctg cagccaaacc
    20581 cccgaagtgc ctaccagcgg tctcgcagga gacttcggct cacccctcac atccggaggc
    20641 aacacccacg ccgcaagtgg gggacatgca ggtactgccg gcaccttcgc gaaaggtggg
    20701 caaccgatga tcggtgcttc gggtgagagc tcggcagcag gttcgagcgc ttacgatttc
    20761 gatacctgtg cgcgcgcgac gctcgaggcc gagcccagca aaacgaacct actgttcgtg
    20821 atcgacaaga gcgggagcat gaactgcaac cctccggacg gagatatcga gcgcgctgcg
    20881 cgctgcgcgc ggtatccggt gcaggaagac aaaactcgcc cttccaagtg gcaagtcacg
    20941 cagacggctc tgatggacgc gatctcaggg ctgagaggac gccccaacct caaggtcggc
    21001 ctgagcttgt ttcccaaatc gaccgcgtgc gattcggcaa gagagcctga tgttacgttg
    21061 gccgatctgg acgatgctca gcttcggcga ctcgaaagca ccctgtcgaa tgtcgccgcg
    21121 agcggagaaa caccgctcgc ggccgccacc attcgtggct atggacatct cgcggatcaa
    21181 ctgaaagcac atacactcga aggtagctcg ctcgtcattc tgctcacgga tggtacggag
    21241 acttgtgcgc ccaacctgct cgacgaccta ttgacgatcc acgccccgaa cgcgcgcttg
    21301 tttcagattc gcacgttcgt aatcggcgtg ccgggtagcg aaggtgcacg cagctttcta
    21361 tccaacctgg cgttcattgg gggtacgtcg gtctcgggat gttcgcaccc cttgttgccg
    21421 aacgatgccg gagattgtca tctcgactcg acgcgcagct tgaacctagc caacgatctg
    21481 aagcaagcac tggagcgcgt gacctcggat cgtgccattg cgtgcacgtt ggatgtgcct
    21541 cgaaacccga cgggtggcgc ggtcgacttc accaaagtca acgtgacttt ttcacctctc
    21601 gggggcgaag ctacgaacat cccctacgag ccgagcgcca cgtgtgacgc tagcgggcgt
    21661 ggttggcaat actcccctga ctttcgacga atcaccttgt gcggcgcggc ttgctcgacc
    21721 gtacaggccc agcccgggac gctcgatgta gtgctgggct gtccaaccga agtcattcgc
    21781 tagtcggcgc tggtctccaa acgcatcctg ctctaaagca gcgcgtatgc agccgacggt
    21841 tttgttgttc gatatcgatg gcacgctcgt gacgacaggc ggggccgggc ggcgcgccat
    21901 gcaacgcagc ttcgatgctt tgtatggtcg acccgacgcc atggacttcg tgatggatgg
    21961 catgacggac
//
LOCUS       DASTKU010000088         5151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1800304, whole genome shotgun sequence.
ACCESSION   DASTKU010000088 DASTKU010000000
VERSION     DASTKU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800304"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFQ61_03060"
     CDS             <1..502
                     /locus_tag="VFQ61_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9953454.1"
                     /translation="RRGDIEDLVQEVFLQVHRSLKDFRFGSRFSTWLYRVTVNVVLMQ
                     RRAARSRPVFVEPPVGFAALDPAASPEDQAARKRRVEAFERLLDRLSDKKRTVYVLHE
                     LEGQSPQEIAKIVGAPVLTVRTRLFYARREIAAGLREEPALSALAEHLDAATLAGGSE
                     PRKEPA"
     gene            499..1824
                     /locus_tag="VFQ61_03065"
     CDS             499..1824
                     /locus_tag="VFQ61_03065"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HET9953455.1"
                     /translation="MKAPWHRKAANGSSSPRASNGNPRHEGLGPSSGSKPELNALTRV
                     LDEMRHAPEPALDWEALESRLMAEIARRSEHGTGVAGPERTGAERNDAERTAAERSRL
                     AGARSSARSASAPRFPGWLWAPTFAAAIGAGLLWLRAHPLPHPPPRETAELRAEGDRV
                     PFADSAAGAPKLTNSSRLDGDALQPGQELRSEAQALVVTHAGRATWRLLPHSRARLVE
                     RAGRIVLELTEGEVSARVVPSPEPGRFIVDAAGARVAVHGTVFNVRLNRDGSVVDVQE
                     GVVSVTPRRAGASVVLLKAPAHGAFTPDGLARDEAPPAPSSPVLAVQPPRAPRKPSDT
                     ATSASSAAALDVPEELTISQVEAGVQPFVAAVEQCFRQNMEPSPALRVSARTTCTLAV
                     NPEGQIEQVTFAPPLAPAVQSCVEEASRDVAFTASRHGIEVTRVLELSR"
     gene            complement(1876..3420)
                     /locus_tag="VFQ61_03070"
     CDS             complement(1876..3420)
                     /locus_tag="VFQ61_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953456.1"
                     /translation="MNEHVTRRGRPSMLGAVGLLALSVTLAIGCQAASEPAPASQGGS
                     GPGPSGNGGNATPLGGGISGGGLGAGGMTAMLPTGMGGTALPNAGTTGDDSCAATTTM
                     AKQIPLELYFITDSSKSMLEQTPTGATKWQALQQAMQGFFNDPNSAGMSVGLKFFPDE
                     QPNVPAACASDAECGAFGPCDQRKACVSKGTRTKDVTALCSADTDCGANEVCVPVQRC
                     MDGANCQKQYCLSGGTGAPACEADCKPFDGYCRGRDVCTATTYGTPAVAPMELPAGAA
                     ALNQALMARTPDGFTPTGPALKGAVQYAQERQRTNPDRKVAIVLVTDGMPGGFIPNMP
                     PEECKPVVIADVAKIVEAGATGMPPVPMFVIGVFGPCDLIDKNIMPQANLDALALAGG
                     TQKSVVISTSAENIAQQLQDALKTIRTTAISCQYALPTQDQAFDVNKVNVNFTSQATG
                     KKVIKYVGTKAACDATEGGWYYDVDPMMALPKQILACDQSCSQFQGAADGRVDILFGC
                     PTVKIF"
     gene            complement(3429..4607)
                     /locus_tag="VFQ61_03075"
     CDS             complement(3429..4607)
                     /locus_tag="VFQ61_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9953457.1"
                     /translation="MSNGGAGNAGGTANVGGTTTATMGGSGDGGGTNPVCQMASYKFE
                     PKIPTVFMLVDQSGSMWGCRASNGTKDPTGRECANKADTSWYPLRDGVLKVVEELEKD
                     VRFGFSAFTGETADAQCPVMQPVAPDFNNYMAIAANYNGLKAPAKGETPTTKALAQVG
                     ALLEADKSPGDKFILFVTDGQPDYCDDSNNLCPPDSVVGALQTLAAKNIKTLVFGISS
                     PLTTISDSVLLAFANAGAGQPVVTPGTTRATDFYDQCNGIAGWRADFMATAKPAERGQ
                     TIGTYSEMNGTAKVYKPDTTNQAALVTELKAAISSAKSCSFDLSNVNGQSIKVDLNQL
                     DAASIAIQGKKIALDEANGWSMASATELVLKGTACETWRSPDNNDIDFNFPCKAIIFE
                     "
     gene            4648..5007
                     /locus_tag="VFQ61_03080"
     CDS             4648..5007
                     /locus_tag="VFQ61_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953458.1"
                     /translation="MFAPPVGAGVVDVPLVEEFPPAIVAPVDDAPALVEPPGELTPAE
                     LPLPPDDVPGLPPLFRPLFGGDEEQAHRAKSDTAAPPNLEKERAERAFRPTESMRWNP
                     PEATDCTGVTQRGVCDN"
BASE COUNT          930 a         1643 c         1599 g          979 t
ORIGIN      
        1 ccgccgaggg gacatcgaag acctagttca agaggtcttc ctgcaagtac accgcagtct
       61 caaagatttt cgattcggat cgcgcttctc cacctggttg tatcgcgtga ccgtcaatgt
      121 cgtgctcatg caacggcgtg cggcccgcag tagaccagta ttcgtagagc caccggtggg
      181 gtttgccgcg ctagatcctg cggcttctcc ggaagatcag gccgcgcgta aacggcgggt
      241 agaggccttc gaacgcctct tggatcgctt gagtgacaag aagcgaaccg tctacgtgct
      301 gcatgaactc gagggacaat caccccagga gattgcgaag atcgtgggcg ctcccgtgct
      361 caccgtccgc acacgtttgt tctacgcgcg gcgcgagatc gcggccgggc tacgagaaga
      421 acctgcactc tcggccctgg ccgagcactt ggacgcggca acactcgcgg gtgggtccga
      481 accgcgcaag gaaccagcat gaaagccccc tggcaccgaa aggctgcgaa cggctcgtcc
      541 agcccgcgcg catcgaatgg caaccctcgg catgagggtc tcgggccgag ctcgggctcc
      601 aagccagaac tgaacgcact gactcgcgtg ttggatgaga tgcggcatgc ccccgagcca
      661 gcgttggatt gggaagcgct cgagtctcgc ttgatggccg agattgcacg ccggagcgag
      721 cacggcaccg gcgtggccgg tcctgaacgc accggtgctg aaagaaacga cgccgagaga
      781 accgctgccg aaagaagcag gctcgccggc gcccgctcgt cggctcgatc cgcaagcgcg
      841 ccgagatttc cgggctggct atgggcacca acctttgccg cagcaatcgg cgcagggctg
      901 ttgtggctgc gcgcgcaccc gctgccccac ccgcccccaa gagagacggc cgagctgcgc
      961 gccgaaggag accgggtccc gtttgcggac agcgccgcag gagcgcccaa gctcacgaac
     1021 tccagccgtc tggatggtga cgctttgcaa cccggccagg agctgcggag cgaagcgcaa
     1081 gcgctcgtgg tcacgcacgc aggacgcgcg acatggcgcc ttctaccgca tagccgcgcg
     1141 cgcctcgtgg agcgcgcggg gcgcatcgtg ctcgagctga ccgagggcga agtgagcgcg
     1201 agagtggtgc cctccccgga gcccggtcgc ttcatcgtcg atgccgccgg ggctcgcgtt
     1261 gcggtgcacg gcaccgtgtt caatgtgcgt ctaaaccgag acggaagcgt cgtcgacgtc
     1321 caagaaggcg tggtctccgt cacgccaagg cgcgccggcg catccgtagt tttgctcaag
     1381 gcgccggcgc acggcgcctt taccccagac gggcttgccc gcgacgaggc gccgcctgcg
     1441 ccgagctcac ccgtgctcgc ggtccaacca ccgcgtgcgc cacgaaagcc gagcgacacg
     1501 gcgacttcgg caagctctgc ggctgcgcta gacgttcccg aggagctgac gatttcgcaa
     1561 gtcgaggcgg gcgtccagcc cttcgtcgct gcggtcgagc agtgcttccg tcaaaacatg
     1621 gagccttcgc cggcgctgcg agtcagcgca cgaaccacgt gcaccttggc cgtgaacccc
     1681 gaagggcaaa tcgagcaagt tacgttcgct ccgccgctcg ccccggccgt gcaaagttgc
     1741 gtggaagagg cgagtcgaga cgtggccttc acggcctctc gtcacgggat cgaggtgacg
     1801 cgcgtactcg agctgtcgcg ttagtgcacg acctccagaa accagaagtc gtacactccc
     1861 gctcacggtt gcaactcaga agatcttgac cgtcggacac ccgaagagaa tatccacgcg
     1921 tccgtcggcg gcgccctgga attgagagca gctctggtcg caggccagga tctgcttcgg
     1981 caaggccatc atcggatcca cgtcgtagta ccagccgccc tcagtggcgt cgcacgcggc
     2041 cttggtgccg acgtacttga tcaccttctt gcccgtggct tgagaggtga agttgacgtt
     2101 cactttgttg acgtcgaagg cttggtcctg agtgggcaac gcatactggc aggagattgc
     2161 cgtagtgcgg atggtcttga gcgcgtcttg tagttgctgg gcaatgttct cggcgctcgt
     2221 gctgatcacg accgacttct gggtaccacc agccagggcc agcgcgtcca gattggcctg
     2281 cggcatgatg ttcttgtcga tcaggtcaca cggtccgaaa acaccgatca cgaacattgg
     2341 gacgggcggc atgcccgtcg caccggcttc gacgattttg gctacgtccg caatgacgac
     2401 cggcttgcac tcttcgggtg gcatattcgg gatgaatcca cccggcatac cgtcggtcac
     2461 gagcacgatc gctactttgc gatctggatt cgtgcgttgg cgctcttgcg catactggac
     2521 ggcgcccttc agcgcgggac cggtcggcgt gaacccgtct ggagttcgcg ccatcaaggc
     2581 ttgattcaaa gccgctgcgc ctgccggcaa ctccatcggt gccacggcgg gggtgccata
     2641 ggtggtcgcc gtgcagacgt cgcggccgcg gcagtagccg tcgaacggct tgcaatccgc
     2701 ttcacacgcc ggagcgccag tgccgccaga caagcagtac tgcttttggc agttggcccc
     2761 atccatacag cgctgaaccg ggacgcacac ctcgttcgct ccgcaatcgg tgtccgcaga
     2821 gcagagggcc gttacgtctt tggtacgagt gccttttgaa acgcaggcct tacgctggtc
     2881 acaaggcccg aacgccccgc actctgcgtc gctggcgcag gccgcgggga cattgggctg
     2941 ctcatcagga aagaacttca agccgacgct catgccagcc gagttcggat cgttgaagaa
     3001 cccctgcatg gcttgctgca aggcctgcca cttggtggcc ccagtcggcg tttgctccaa
     3061 catggacttg gagctgtccg tgatgaagta gagctcgaga gggatttgtt tggccatggt
     3121 ggtggtggct gcgcatgagt cgtcaccggt ggtgccggca ttcggcagcg ctgttcctcc
     3181 cattccggtg ggcaacatgg ccgtcatgcc ccccgcaccc aaaccgccgc cgctgattcc
     3241 accacccaag ggagtggcat tgccgccgtt tcctgacggg cctggcccgc tacccccttg
     3301 cgaagcaggc gcaggctcac tggcagcctg gcagccgatg gccagagtaa ccgataatgc
     3361 caacaaccct accgcgccca acatcgaagg ccggcctctt ctcgtgacgt gctcgttcat
     3421 gaattggcct attcgaagat gatggctttg caggggaagt tgaagtcgat gtcgttgtta
     3481 tcgggagagc gccacgtttc acacgcggtg cctttgagta cgagttcggt cgccgacgcc
     3541 atggaccaac cgttggcctc gtcgagtgcg atcttcttgc cttgaatcgc gatgctggcc
     3601 gcgtcgagct ggttcaaatc cactttgatg gattgaccgt tcacgttcga gagatcgaag
     3661 gagcaactct tggccgaaga gatcgccgct ttgagctcgg tgacgagggc cgcttggttg
     3721 gtcgtgtccg gcttatatac cttggccgtg ccgttcatct ccgagtaggt cccaatcgtt
     3781 tggcctcgtt cggccggctt ggccgtagcc atgaagtcgg cgcgccagcc cgcgatgccg
     3841 ttgcattgat cgtagaagtc cgtggctcgc gtcgttcccg gcgttaccac gggttgacct
     3901 gcaccggcgt tcgcgaacgc caacaatacc gaatccgaga tcgtcgtcag aggcgagctg
     3961 atcccgaaaa ccaaggtttt aatgttcttc gcggcaagcg tttggagtgc tccaaccacc
     4021 gaatcgggcg ggcaaagatt attcgagtcg tcgcaatagt ctggctgccc gtcggtgacg
     4081 aacaagatga acttgtcacc cggtgacttg tcggcctcga gcaaggcacc tacctgcgcc
     4141 agcgctttgg tggtcggcgt ctcgcccttg gccggtgcct tgaggccatt gtagttcgcc
     4201 gcgatggcca tgtagttgtt gaagtccggc gctacgggtt gcatcacggg acactgcgcg
     4261 tccgcggttt cacccgtgaa agcactgaag ccgaagcgca cgtccttctc gagctcttcc
     4321 acgaccttga gcactccgtc acgcaagggg taccaagaag tatccgcctt gttcgcgcac
     4381 tcgcgtccgg ttggatcttt ggtcccatta ctggcccggc agccccacat gctgcctgac
     4441 tgatccacga gcatgaacac agtcgggatc ttgggctcga atttatagct cgccatctgg
     4501 catacggggt tcgtacctcc gccgtctcct gaaccgccca tggtggccgt cgtcgtgccg
     4561 cccacgttcg cggtgccacc cgcgttgccc gcgccgccgt tggacacggc acctgcaccc
     4621 gcagcgccac tgccagcttg gcccgtcgtg ttcgcaccgc cagtgggtgc cggggtggtc
     4681 gacgtaccgt tggtcgagga gttcccaccg gccattgtcg cgcctgtcga cgatgcgccc
     4741 gcgctcgtgg aaccacctgg agaactcaca cccgcggagt tgccacttcc gccagacgac
     4801 gtaccgggat tgcccccgtt gtttcgtccg ctgttcggcg gcgacgaaga acaagcccac
     4861 agagccaaga gcgacacggc ggctccaccc aatctcgaaa aagaacgagc cgagcgggcc
     4921 ttcagaccaa cggaaagcat gcggtggaac cctcctgaag ccacggattg cacgggcgtg
     4981 acgcaacgcg gagtttgcga caattgaagc aagcagcgaa cgcaccttct agcatggttt
     5041 tacacgctcg cgaaggcgcg cggctgaatc ccaattcgta aactacgttg agcaacgccc
     5101 gctgtttatt ggtccgtgct catatcgctc gtattgtccg cgcttactcc c
//
LOCUS       DASTKU010000089        11088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1355427, whole genome shotgun sequence.
ACCESSION   DASTKU010000089 DASTKU010000000
VERSION     DASTKU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11088
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355427"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..2385)
                     /locus_tag="VFQ61_03085"
     CDS             complement(91..2385)
                     /locus_tag="VFQ61_03085"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HET9953459.1"
                     /translation="MAENTRNPSLNTVPESSSGLSPSAILRALRKNWLLGVGVALTVL
                     LAVTFFTLGQTKIYRASAMIEIDPSPPRPLGDQVQGVVDMGAGTYLNNREYYETQYAI
                     IKSMRLAVAVVRDLALNRDIAFIDNLPRGTKPVPREVSEETAAQVLRARVEVEPQKQS
                     RLAKISYEDADPERAQRITAALIRRFQEQNLDSVLSSTTAAVEWLDDQLQKLSAELET
                     SELALHKYKMNRNILSVSIDDQSNMLREEMRQLNTALTDARTRQQELMARYKALSEVT
                     STNPEDVPATELMNNQLISKLRVDYIDFTRRLGELKGSGKGEQHPSVVEVREKAEITK
                     QALLAEVENVKRAVERDLSIVKRQAEGLRSLYENANKRALELNLLEIEYNTLRRTRDN
                     TQKLYSVVQERAKESDLTRMLRVNNISVVDPPLLPRSPAKPSVPVSLIIGLFAGSVLG
                     LGAALGRELLDRSIKVQEDLEHELGLTFLGMLPQAEGLGFDAGPGKKKSRRRAPKSET
                     DISGKPELVVHEHPTSGLAEAARALRTNIMLMAPDRPYRALLVTSAAPEEGKTMFACS
                     IATAMAQAGQKVVLVDCDMRRPRLHRIFGRAGGLGITTAMLDLGRLSDCLIPSPVPNM
                     FVLPCGPLPPNPAELLHSEAFLRVLEELKSRFDRVVIDSPPIAPVTDATILSTRVDGT
                     LLVVRAFKTTKDLARQCARSIRDVGGHIVGAVLNAVDVERRHYAQYYQYYYYRHEGYG
                     TLEEQASSESRGTNPPASPPANVG"
     gene            2539..3675
                     /locus_tag="VFQ61_03090"
     CDS             2539..3675
                     /locus_tag="VFQ61_03090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9953460.1"
                     /translation="MFSAAGWPGSGFCALRIALNFATARGAGSSRVAKGILGGLQSLR
                     APHVYLVFVPEQWPASPMLDGRFCVHKSRSGVFAKWAHESIRLPLEMRQGGFDKLFSG
                     QDTGPPWPLVPELLLVQQAYLAYSPAEWGFTVGGRFALRMQLMARYFQMGLPRVRLFT
                     VQTEDMRTHLVNRWQIDPARIHVVPSSADFVVRRAPVPHATPPYLIYVSAAAPHKNYL
                     VLAEMLAILRGEFPELKLKLTTREEEVPEFVARAKALGVHSQVEYLGIMKREALEREL
                     AEAAAAVIPSKLESFGLTYYEALALGVPVVAADLGFAREACGEAGLYAHPDAGEDFAA
                     GVRRLISSAETRADYVQRSLKRFDEVNVSWSEIASRYLALIERL"
     gene            3678..6203
                     /locus_tag="VFQ61_03095"
     CDS             3678..6203
                     /locus_tag="VFQ61_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953461.1"
                     /translation="MAHEGGTSRRSAQAWTKPELVAAALCGAAVLCSALGLAGLSLIV
                     LVPVSMAALAAFVLVMPRRDLAAPSLVFFAAAGFTLIQAMPLPAGVVHAIAPRNHWIW
                     TGAYEALGQATPRWISISLDPSASWIEVLKWLSYGAVFGAAAVLGGRRGGLWGAYAVW
                     IAALTVAFATLLHGLAGATRVYGLYEPTFQPDRWNMGPMLNGNNLSGYLNLGALSGLG
                     LCASRRARYRAPIAVGTAVLMAMSVLSASRAGTATLVLGVVVFAVLKSRLQEHSSPDR
                     LGRLLLWGTLGFALLLGVVGASRATWSALSDASTDKLDIMRWGANMVGDHFAVGVGRG
                     AYGSASFAFTHSERGLLYEYPENILVQWGAEWGAPFALVLAASFWRTLKPWGLAFKTS
                     TVASGVCAGVFALLAQNLADFSLEVPAVGYAVALALGGCWGEYRSRRGIKPIPQRAAL
                     GLAWGVGAMGAALVILVVGSQARSASRARPALHDVYRDANLSDGPARQALQTRIRADI
                     RMYPADPYFPRLMALVAYRSASENPLPWINRALELAMSSGRTHLLLARMLLTYGRLEQ
                     ALLELRLAAEAEADLTGRAAGIAMQYTRDPELLTRAVPMGAAGAQALTQFAVRLNRPE
                     EAQVRLRMLHEAQRRAPNEHLAWVIELQDSLTELGPGARTERCKEPEPGSANENPREA
                     CIRAAERLLLKIEKLMPGSSIPLELRAQWLSAAGRIAEAERLLAKGCRELVLPISCLR
                     AWMDSAIRARAWSAADEAAKLILSQSCAETSRCAVTAEQLGDRLVQLGAPAAALPIYA
                     RAAREDATAGRWMKVSKVATQLGMEAQAREARIRAKQINAATP"
     gene            complement(6276..7943)
                     /locus_tag="VFQ61_03100"
     CDS             complement(6276..7943)
                     /locus_tag="VFQ61_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953462.1"
                     /translation="MGPNPHPEPNGGSATQHAGGQAGQTDSNRGGTGANPSPGSSGEP
                     GASGAGAGGTESSESSQTGRWLVYTHTFSDNPYEVPENVLSTTAAIDLSRPNTLPIEL
                     GTSPYAVPAPSFSPDGHWLVHGIGTQTVLRQVRGEGFGAPTSLPGCEGQSSEVTWSAR
                     GTWLACLSSTPTAQLYSISRVDTGGERRLVDARSCSPRFAPDDDHIVITCFVRAPEPQ
                     PGKSVLRVESLTGSGAAGIVENVFWVDWLSPTSALLRNTDGRFGVLRFGGQEAIGVDW
                     FAVDESVSFNGNNMLRHSRDGTRALLETRSSTCAVLDTVPGRLAGVYDCHLVSTDLDR
                     VAVVNPERGEVEVRLLTQTPNPVLFTVPGTEVLGWSDDDRLLFTKRLSSAELLLTQLE
                     SVRRTATISPASWDDEGLPDLVEAVNRIKVAPNNAWAVAYGEQDSAYVDLQEEPWFRA
                     TGVYGYGSTQTVDISPDSGAFAIVADYGTGLGRGWGRLAEAREYAWVPRFGAVISSPY
                     PQPQTVREPGSVRIDYYSDEGPMGLVPFRGYQNAKHQDLLQGFAMQP"
     gene            complement(8199..9827)
                     /locus_tag="VFQ61_03105"
     CDS             complement(8199..9827)
                     /locus_tag="VFQ61_03105"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9953463.1"
                     /translation="MVEGTPTRASSDEETTHELLGRPDVPDSGSDDLVGHLLNETYRV
                     QRVLGEGGMGRVYLAQHARIEQKRVAIKVLHPEHALNAEAAARFRREAEAAAAISHPN
                     VVAVFDVDRTPEGRLYIVSEYLEGIELAAHLDNVQRLPAAHALHIARQLCRGLAAAHA
                     RGVIHRDLKPQNIFLVGDFKDGVPAQPHVKVLDFGLSRFVEGAAQVQLTKTGVIMGTP
                     SYMPPEQALGQRVDTRADIYGVGAVLYRALTGRPPFAEDTPQATVLAVIHGEPARPRS
                     VEPSIPPELEIVLERALARDPAQRYPDMQALDLALKSIESEPPERRESFPARALLLPT
                     PPRPGSVGEFEVEDVRSARLRLAMAVGFTVILVCAALGLAVTGLEQAVGYRFKPLELR
                     LVFLAILGSALTPAVLWLARIRRRVWENSSRVLGLLGDLRSAVLWATASYGLGVIVLH
                     LVNDLGPRLVRRVPWPELDPAWLGWNLVLPCVAWLVAVSAVVRRKVARSVRPGWRRLF
                     AVGLVSAVALAALAGTGYAGVRWRLNTSSNTPSR"
     gene            10048..10869
                     /locus_tag="VFQ61_03110"
     CDS             10048..10869
                     /locus_tag="VFQ61_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9953464.1"
                     /translation="MDPLINRRQLLRGSGALLLAAGVDGLAIEPRWLDVTSHELPIAS
                     LPRELEGFTLAQITDAHLTSLSRVESAVLQRLQSTDAQLVVLTGDMIDCERHLVDLKH
                     FCDALRKPGRRLVATLGNWEYWGKVPLDLLRRAYEDVGVNLLVNESLVLPEGLSVSAT
                     DDFTAGSPDLHHVEPNRAAHASILLTHSPQLLDSIPELEHSFQLTLAGHTHGGQVRLS
                     QHFAPVRPPGSGRFVSGWYDTRAGRAYVSRGTGTSIVAARFSCRPELPLFTFRQA"
     gene            complement(10876..>11088)
                     /locus_tag="VFQ61_03115"
     CDS             complement(10876..>11088)
                     /locus_tag="VFQ61_03115"
                     /inference="COORDINATES: protein motif:HMM:NF020691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leukotriene A4 hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HET9953465.1"
                     /translation="SLALQSGYHAVLGRVEQVVGAVGRMKYLRPLYTNLIADARTRPM
                     ATRLYEKNRATYHPIARQMVEGLLSR"
BASE COUNT         1949 a         3497 c         3514 g         2128 t
ORIGIN      
        1 cgctgggcgg tccgtatacc tacttcgacg gtgatttggc ggcattccgg ctgtatcgag
       61 gggccctgag tgaccccaag attcgagagc tcagccgacg ttagcgggag gggaagcggg
      121 aggattcgtt ccgcgtgact ccgacgacgc ttgttcctcg agcgtgccgt agccctcgtg
      181 gcggtagtag taatactgat aatactgcgc gtaatgacgg cgctctacgt ccacggcatt
      241 gagcaccgcg ccgacgatgt gcccacccac atcgcggatc gagcgggcac attggcgcgc
      301 caagtctttg gtcgtcttga acgcgcgaac gaccagcagc gtaccgtcca cgcgggtcga
      361 taggatagtg gcgtccgtaa cgggcgcgat gggcgggctg tcgatcacga cgcggtcgaa
      421 gcgagacttg agttcttcga gcacccgcaa gaaggcttcg ctgtgcagga gctcagccgg
      481 attgggcggc aggggcccac acggcagcac gaacatgttc ggtaccgggg aggggatcaa
      541 gcaatccgac agtcgcccga gatccagcat tgccgtcgtg atgccgagcc cacccgcgcg
      601 cccgaagatg cgatgcagac gcgggcgacg catatcgcag tccaccagca ccaccttctg
      661 tccggcttgt gccatcgcgg tggcgatgct gcaggcgaac atcgtcttgc cttcttcggg
      721 cgctgcgctc gtgaccaaca acgcgcgata cggacgatca ggcgccatca gcatgatgtt
      781 cgtgcgcagc gcgcgcgccg cttcggcgag gccgctggtc gggtgctcgt gtacgaccag
      841 ctctggtttg ccggagatat ccgtttcgct cttcggcgct cgacggcggc tcttcttctt
      901 gccgggaccc gcatcgaagc ccaggccctc ggcttgcggc agcatgccga ggaaggtgag
      961 cccgagctcg tgctccaagt cttcctgaac cttgatgcta cggtccagca gttcccgacc
     1021 gagcgcggca ccgagcccga gcacgctgcc cgcaaacagc ccgatgatca agctcacggg
     1081 tacgctcggc ttggccggcg agcgaggcag tagcggcgga tcgactacgc tgatgttgtt
     1141 gacacgcagc atccgcgtca ggtcactctc tttggcgcgc tcctgcacca ccgaatagag
     1201 cttctgggtg ttgtcccgcg tgcgccgtaa cgtgttgtac tcgatctcga gtagattcag
     1261 ctccagcgcg cgcttgttgg cgttctcgta caagctgcgc aacccttcag cctgccgctt
     1321 gacgatgctc aggtctcgct cgaccgcgcg tttgacgttc tctacttcgg cgagcagggc
     1381 ttgcttggta atctcggcct tctcgcggac ctccaccacg ctcgggtgct gctcgccttt
     1441 gccggagccc ttgagctccc cgagccggcg cgtgaaatcg atgtagtcca cgcgcaactt
     1501 cgagatgagc tggttattca tcagctcggt ggcgggcacg tcctcggggt tcgtggacgt
     1561 cacctccgag agcgctttgt agcgcgccat cagctcttgt tgtcgcgtgc gcgcgtcggt
     1621 caaggccgtg ttcagctggc gcatctcctc gcgcaacatg ttcgactgat catcgatcga
     1681 caccgacaag atgttgcggt tcatcttgta cttgtgaagc gcgagctcac tcgtctccag
     1741 ctccgcgctc agcttctgca actgatcgtc gagccactcc acggcggcgg tggtagagga
     1801 aagcaccgaa tccaagttct gttcttggaa tctccgaatc agagcggccg tgatgcgctg
     1861 ggctcgctcc ggatcggcgt cctcgtagct aatcttcgcc aaacggcttt gcttctgagg
     1921 ctccacctcg acgcgtgcgc gcagcacctg cgccgcggtc tcttcactca cctctcgcgg
     1981 cacgggcttg gtgccgcggg gcaagttgtc gatgaacgcg atgtctcggt tcaacgcgag
     2041 gtcccgcacg actgcgaccg ccaagcgcat cgacttgatg atcgcgtact gggtctcgta
     2101 atactcgcga ttgttcaggt aagtgccagc gcccatgtcg accacgcctt gcacctgatc
     2161 gccgagggga cgcggtggag acggatcgat ttcgatcatc gccgacgccc ggtagatctt
     2221 cgtctgaccg agggtgaaaa aggtgaccgc tagcagaacc gtgagcgcaa cacctacacc
     2281 cagcagccag ttcttcctaa gcgctcgaag gatcgcgcta ggagagagtc ccgaggacga
     2341 ctcgggaacg gtattcaacg aaggattacg ggtattctcg gccactttgt tttcactcgc
     2401 cggtcggtat tctgtggtaa cgccgttgcc ctggaccgaa cggatgcctg gatgtagcga
     2461 aatattcagc tcacggcgag aaagatggcg aattctcgaa cttagttttg agacgggggc
     2521 gcaaccgtta cacatatcgt gttttcagcg gccgggtggc cgggaagcgg attctgcgct
     2581 ttgcgtatcg cgctgaactt tgccacggcg cgcggcgccg gcagctcacg tgtcgcgaag
     2641 ggcattctcg gaggcttgca gtcgctgcga gctccgcacg tttacttggt tttcgtgccc
     2701 gagcagtggc ccgcgtcgcc gatgctggac ggtcgattct gcgtacacaa atcgcggtcg
     2761 ggggttttcg ccaagtgggc tcatgagtcc atccggcttc cgctcgagat gaggcagggc
     2821 gggttcgata agctcttttc cggtcaggac acagggccgc cgtggccgct cgtgccggag
     2881 ttattgctgg ttcagcaagc gtatctcgct tactcgccgg ctgagtgggg atttacggtg
     2941 ggcgggcggt tcgcgctccg catgcagctg atggctcgct atttccagat gggattgcca
     3001 cgagtccggc tcttcacggt tcaaaccgag gacatgcgca cacatctcgt caatcgatgg
     3061 cagatcgatc ctgcgcgaat tcacgtagtg ccgagcagcg cggacttcgt agttcggcgt
     3121 gctcccgtgc ctcacgctac acccccctac ttgatttacg tctcggccgc cgccccccac
     3181 aaaaactatc tagttctggc ggagatgttg gccatactgc gcggcgagtt tcccgaactg
     3241 aagctcaagc tcaccacgcg agaggaagaa gtgccggaat tcgtggcgcg cgccaaggcc
     3301 ttgggcgtgc attcacaggt cgagtatctg gggattatga agcgggaggc gctcgagcgc
     3361 gagttggccg aagcggctgc ggcggtcatc ccatccaagc tcgagtcctt cggtctgacg
     3421 tactacgaag cgctcgcgct cggggtcccc gtggttgccg ccgatctcgg gtttgctcga
     3481 gaagcctgcg gcgaggccgg gctctacgcc cacccggacg caggcgaaga cttcgcggca
     3541 ggcgtgcgac ggctcattag ttctgcggaa acacgcgcgg actacgtgca gcgctcgctg
     3601 aaacgcttcg acgaggtgaa cgtatcgtgg tcggagattg cgagccgata tctcgcgttg
     3661 attgaacgtc tgtagctatg gcgcacgagg gaggaacttc gaggcggtct gcccaggcat
     3721 ggacgaagcc ggagctcgta gcggcggcgt tgtgtggggc ggcggtactg tgctctgcgc
     3781 tcgggctcgc gggcttgagc ttgatcgtgc tggtgccggt gtccatggcg gcgctcgcgg
     3841 cgttcgtgtt ggtcatgccg cgtcgggatt tggcggcccc gagcttggtg ttcttcgccg
     3901 ccgcgggctt cacgttgatt caggcgatgc cgcttccggc tggcgtcgtt cacgcaatcg
     3961 ccccacgtaa ccattggatt tggaccggcg cctacgaagc gctggggcaa gcaacccccc
     4021 gatggatcag catctccctc gatccgagcg ccagctggat cgaggttttg aagtggttat
     4081 cctacggtgc ggtgttcggc gcggctgccg tgttgggtgg gcgccgcggc ggtttgtggg
     4141 gcgcctacgc cgtgtggatc gcagcgctga cggtggcgtt tgcgacgctg ctacatgggc
     4201 ttgcgggcgc gacacgcgtg tacgggcttt acgagcctac gttccaaccg gatcgctgga
     4261 acatgggccc gatgctgaat ggcaacaatt tatctggtta cctgaacctg ggtgctttgt
     4321 ccggcctcgg cctatgcgcg tcgcgacgcg cgcgctaccg ggctccgatc gccgtcggta
     4381 cggcggtgtt gatggcaatg tccgtgctgt cggcgtcgcg tgccggaacc gcaacgctgg
     4441 tcttgggtgt ggtcgtgttt gccgtgctca agagccgact gcaagagcac tccagtccgg
     4501 acaggcttgg gcgcctgttg ctgtggggga cgcttggttt cgcgctcttg ctcggagtag
     4561 tgggcgcgag ccgtgccact tggagtgcgc tgtccgacgc gagcaccgac aagctagaca
     4621 tcatgcgctg gggcgcgaac atggtgggag accacttcgc cgtcggcgtg ggtcgtggcg
     4681 cttacggcag cgcttcgttc gcgttcaccc actccgagcg tggcttgctt tatgagtatc
     4741 ccgaaaacat tttggtgcag tggggagcgg agtggggggc gccgtttgcc ttggtgctcg
     4801 ccgcttcttt ctggcgaacg ctgaaacctt ggggattggc gttcaagacc tccaccgtgg
     4861 cttccggcgt atgcgcggga gtgttcgcgc tcctggcgca aaatttggcg gacttcagct
     4921 tggaagtccc cgcggtgggt tatgcggtgg cgctcgctct cggcggctgt tggggagagt
     4981 atcgctcacg ccggggtatc aagcccattc ctcaacgcgc ggctctcggc ttggcttggg
     5041 gagtgggtgc aatgggcgcg gcgctcgtga tcctcgtggt cggctcccag gcgaggtccg
     5101 catcgcgcgc ccggcctgcg ctgcacgacg tgtatcgcga cgcgaacttg agtgacggtc
     5161 cggcgcgtca agccctacag acgcgaattc gtgctgatat ccgcatgtac ccggcggatc
     5221 cttacttccc tcggctcatg gctttggtcg cttatcgcag cgcgagcgag aatccgctgc
     5281 cttggatcaa ccgcgcgctg gagctcgcaa tgtcgagcgg tcggactcac ctcttgctcg
     5341 cccggatgct gctaacgtac gggcggctcg aacaagcctt gctggagctg aggctggcgg
     5401 cggaagccga ggctgacttg acggggcgcg cggcaggaat cgcgatgcaa tacactcgtg
     5461 accccgagtt gctgacccgt gcggtcccga tgggagcagc gggcgcgcaa gcgctcaccc
     5521 agtttgccgt ccgcttgaat cgtcccgaag aagctcaagt gcgtctgcgc atgctgcatg
     5581 aagcacaacg tcgcgccccg aacgagcacc tggcctgggt tatagaactg caggactctt
     5641 tgacggagct cgggccaggt gcgcggacgg agcgctgcaa agaaccggaa ccgggtagcg
     5701 ccaacgagaa cccgcgcgaa gcctgcattc gcgccgccga acggctcctc ctgaagatcg
     5761 aaaagctgat gccgggcagc tccatcccgc tcgagttacg agcgcaatgg ctttcggcag
     5821 ctggcagaat cgctgaggcg gaacgcctat tggcgaaagg gtgccgcgag ctggtgcttc
     5881 ctatctcgtg tctacgggca tggatggata gtgccatccg agctcgcgct tggagcgccg
     5941 cggacgaggc tgccaagctc atcctgtccc agagctgcgc ggaaacatcg cgctgcgccg
     6001 taaccgccga gcagctcggc gatcgtctcg tacaactcgg ggcccccgcc gcagcccttc
     6061 ccatctacgc gcgtgcggcg cgggaggacg ctaccgcggg tcgctggatg aaagtctcca
     6121 aagtcgccac ccagctcggc atggaggcgc aggctcgaga ggcccgcatt cgagccaaac
     6181 aaatcaacgc ggccacgccg tgatcgccga cttcccccgt cggcgcgcaa tctaaagctc
     6241 aagggcactc gcccctgcat tgatcgccgg ttcgcctaag gttgcatcgc aaaaccctgg
     6301 agcagatcct gatgcttcgc gttctggtaa ccgcggaacg gcacgagccc catcggtcct
     6361 tcatcggaat agtagtcgat tctcacgctg cccggctctc gaacggtttg aggttggggg
     6421 tagggcgagc tgatcacggc tccgaaacga ggcacccaag cgtactcgcg tgcctcggca
     6481 agccttcccc agccccttcc caggccagtt ccgtagtcag ccactatcgc gaaagcgcca
     6541 gaatctgggg aaatgtcgac ggtctgagtg ctgccgtagc cgtagacccc ggtcgccctg
     6601 aaccacggct cttcctgcag gtccacgtac gcggagtcct gttcgccgta ggccaccgcc
     6661 caagcattgt tcggcgcaac tttgattcga ttcaccgcct caacgaggtc gggcagacct
     6721 tcgtcgtccc acgacgcggg cgaaatggtt gcggtacgcc gcacgctctc caactgggtg
     6781 agtagcaact cggcgctact cagccgtttg gtgaacaaaa gtcgatcatc gtcggaccag
     6841 cccagaacct ccgtacctgg aacggtgaag aggaccggat tgggcgtttg cgtcagcagg
     6901 cgcacttcca cctcgccgcg ttccgggttc acgacggcaa ctcggtccaa gtcggtcgag
     6961 acgaggtgac aatcgtatac tcctgccaaa cggcccggca cggtgtctag aactgcgcag
     7021 gtcgaggatc tggtctccaa caacgctcgg gttccgtcgc ggctatggcg gagcatgttg
     7081 ttcccattga aagagacact ctcgtccacg gcaaaccaat cgactccaat tgcttcttgc
     7141 cctccgaagc gcagcacacc aaacctgcca tccgtgttcc tgaggagcgc actcgtggga
     7201 gagagccagt cgacccagaa gacattctcc acgatcccgg ccgctccgga tccggtgagc
     7261 gactctacac gcagtacgct tttccctggt tgcggctcgg gcgccctcac gaaacacgta
     7321 atgacgatat ggtcatcatc tggggcgaaa cgcggagaac aagaacgcgc gtcgacgagc
     7381 cggcgctcgc ctcccgtgtc cacgcgagag attgagtaga gctgggccgt cggcgtcgag
     7441 gacaggcaag caagccaagt tccccttgcc gaccacgtca cttcgctgct ttgcccctcg
     7501 cacccgggca aagaagtcgg tgcgccgaat ccttcgccgc gcacctgccg taaaacggtt
     7561 tgtgtcccga ttccgtgaac gagccagtga ccgtcgggcg agaaggacgg cgcgggcacg
     7621 gcgtaggggg aggtgccgag ctcgatcggc aatgtattgg ggcgagatag atcgatcgcc
     7681 gccgtggtgc tcaggacgtt ctccggcacc tcgtaagggt tatcgctgaa ggtgtgcgtg
     7741 taaaccagcc agcgccctgt ctgcgagctc tctgaactct ccgtgccgcc tgcgcccgca
     7801 ccactcgccc ctggctctcc gctcgagccg ggggatgggt tcgcgcccgt acccccgcga
     7861 ttcgaatccg tctggcccgc ctgacccccg gcatgttgcg ttgcgctacc gccattgggc
     7921 tccggatgcg ggttgggacc catgcttccc ccgcatgcgg cgcaatgcgt ggcgaggacc
     7981 caaatcgtag cgcgcatatg ccatacggtt tcgaattgat cgcctcgact tcgctcgatc
     8041 ggcacgtgac tccctccaca gctcttgcgg ctcacgaagt atccgcaatt cagtgaattg
     8101 gtcagctggc gatatccccc atggagtgag cgaatgcggg ccctttccct gggagtgatc
     8161 gagtgccaat ccgttaatcg gactgaaacg tgctcgcctc aacgtgaggg agtgttagat
     8221 gaggtgttca gtcgccaacg cacccctgcg tagcccgttc ccgccagcgc cgcgagcgcg
     8281 accgccgaaa ctaaaccgac ggcgaatagg cgacgccagc cgggacgaac cgaacgcgct
     8341 accttgcgac gaactaccgc cgagaccgcc acgagccaag ccacgcacgg taaaaccaga
     8401 ttccatccga gccacgccgg atcgagttct ggccagggca ctcttcgaac gaggcgcgga
     8461 cccagatcgt tgaccaagtg caatacgatc actccgagcc cgtagctcgc agttgcccac
     8521 agcaccgccg agcgtaagtc ccccaataac ccgagcactc gcgagctatt ctcccaaacc
     8581 cggcgacgga tgcgcgcgag ccacaacacc gcaggcgtca aagcgctgcc caggatcgcc
     8641 aagaacacga gccgcagctc gagcggcttg aagcgatagc ctacggcctg ttcgagcccg
     8701 gtcacggcca agcccaacgc tgcgcacacc aggatgaccg tgaaccccac ggccatcgcc
     8761 aagcgcaggc gcgcagagcg aacgtcttcc acctcgaact cgcccaccga gccagggcgc
     8821 ggtggcgttg gcagcaagag cgcgcgagcg ggaaaggatt cacggcgttc gggtggctcg
     8881 ctctcgatgg acttgagcgc caaatcgagc gcttgcatat cggggtagcg ctgagcggga
     8941 tctctggcga gcgcgcgttc gagcacaatc tcgagctcgg gcgggatact cggttcgact
     9001 gagcgaggcc gggcaggttc accatgaatc acggccaata ccgtggcttg cggtgtgtcc
     9061 tcggcaaacg gcgggcgccc ggtaagagcc cgatacaaca ccgcacccac cccgtaaatg
     9121 tctgcgcgcg tgtccacgcg ctgccccaga gcctgctcgg gcggcatgta cgaaggagtc
     9181 cccatgatga cccccgtctt cgtcaactgc acttgagcgg cgccttcgac gaaacgagat
     9241 agcccgaaat ccagaacttt gacgtgaggc tgggcgggca ccccgtcctt gaaatcgccg
     9301 accaagaaga tgttctgcgg cttcaagtcg cgatggatca caccgcgcgc gtgggcggcg
     9361 gccagaccac ggcagagctg gcgcgcaatg tgtagagcat gcgcggccgg tagccgctgc
     9421 acgttgtcca gatgggccgc gagctcgatg ccttccaggt actcgctcac gatatacaat
     9481 cgcccctcgg gggtgcgatc cacatcgaag acggccacca cgttcggatg agagatggca
     9541 gcggcggcct cggcttcacg tcggaagcgc gccgccgcct cggcattcag cgcgtgttcc
     9601 ggatgcaaga ccttaatcgc cacccgcttc tgctcgatgc gtgcgtgctg ggccaggtag
     9661 actcgaccca tgccgccttc gcctagcacc cgctgcaccc gataggtctc gttcagcagg
     9721 tgccccacca gatcgtcgct gccgctgtcc gggacgtctg ggcgccccaa cagctcgtga
     9781 gtcgtctctt catcgctgga cgcgcgagtc ggggtacctt cgaccattct ggccccagcg
     9841 ttctgacaca ggcggcccag gaatgggaat cccaacgtaa cgtcgttcca gcaaacggct
     9901 ccgattccga cgtgccgtga aaagttgcgc ggctcgcgcc ggcgctactg tgctccggcc
     9961 gcacggcacc tacgagcgcc gctacagtgg ctgtttagga gggcaatggt caccaggcag
    10021 ccgaaaccat gccatcttgt gcgcgtgatg gatccgctca tcaaccggcg acagctactg
    10081 cgcggctcgg gtgcattgtt gttggcggcc ggagtggatg gtctcgcgat cgagccgcgc
    10141 tggttagacg tgacgagcca cgagctaccg atcgcgtcgc taccgcgtga actcgagggc
    10201 ttcactctcg cgcagatcac ggacgcgcac ctgaccagct tgagtcgcgt cgaaagcgcg
    10261 gtcctgcagc ggttgcagtc cacggacgcg cagctcgtgg tgctgaccgg tgacatgatc
    10321 gattgcgagc ggcacctggt cgatctcaag catttttgtg acgcgcttcg aaagcctggg
    10381 agaaggctgg tcgcgacgct cggaaactgg gaatactggg gtaaggtccc gctcgatttg
    10441 ctgcggcgcg cctacgaaga cgtgggcgta aacctactgg tgaatgagtc actcgtgctc
    10501 cccgaggggc tctcggtatc ggccaccgac gacttcaccg ctggctcccc ggacctgcat
    10561 cacgtggagc cgaaccgtgc agcccacgca tcgatcctgt tgacgcacag cccgcaacta
    10621 ctcgactcga ttcccgagct cgaacactcg ttccagttga ccctcgccgg gcacactcac
    10681 ggcggccaag tccggctgag ccagcatttc gcccccgtac ggccgccggg tagcggccgc
    10741 ttcgtttcag gctggtatga cacccgagcg ggccgcgctt acgtgagccg cgggacaggc
    10801 acgagcatcg tcgccgctcg cttctcttgt cgaccagagc tgccgttgtt cacgtttcgc
    10861 caggcctgag aacagtcaac gacttagaag tccctcgacc atctgccgcg cgatcgggtg
    10921 gtaggttgcg cggttcttct cgtagagccg agtggccatc ggtcgcgtgc gggcgtctgc
    10981 aatcagattc gtatagagcg ggcgcaggta cttcatgcgt ccgaccgcgc caaccacctg
    11041 ttcgacgcgg ccgagtacag catgatagcc ggattgcagg gcgaggct
//
LOCUS       DASTKU010000090         8117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1577999, whole genome shotgun sequence.
ACCESSION   DASTKU010000090 DASTKU010000000
VERSION     DASTKU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577999"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1221
                     /locus_tag="VFQ61_03120"
     CDS             28..1221
                     /locus_tag="VFQ61_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953466.1"
                     /translation="MKRVVWFLSLLWGVGALGAVGCGNIDTPLGELPPVDGAKCLLQG
                     VTAAAPDGCNTCSCGSDGLWDCTEKDCSGVCMPGATKLSEDGCNACSCSADGSGWECT
                     TKDCAQTCAPGARKPAGDGCNSCVCSPDGTSWDCTLKSCTMQCKPGDTKLAEDGCNDC
                     FCDESGSWACTLVDCAPECRPGAMKIVEDGCNECLCSSDGRWSCTDKACMTECRPGAM
                     KIAEDGCNECLCSSDGRWSCTDKACMTECRPGEMKSSGDGCNSCFCDQSGHWACTARD
                     CAPECTEGESKPAGDGCNTCSCVSGRWSCTMRACMPQCKPGEKTFDGCSSCACDDAGR
                     WLCTANYCGECPPPAMVPSAQCAGGAVFAKNPEFGTCCAYDAACQAPSDWKTFSTQSA
                     CAYAE"
     gene            complement(1280..1747)
                     /locus_tag="VFQ61_03125"
     CDS             complement(1280..1747)
                     /locus_tag="VFQ61_03125"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET9953467.1"
                     /translation="MSAGSGASTQASGDGRSGESGEELLFEGRPALIPSLGALLLTIL
                     TLGLYLLPRWWQRRGVHYRVTTRRIVVETGVLSKRLEQIDLYRIADYTAERPFAQRLL
                     GTGNLLLKTFDKTTPELCLQAIPTDVVELYERVRAATELEKSRRGVRVVDQEA"
     gene            1858..3486
                     /locus_tag="VFQ61_03130"
     CDS             1858..3486
                     /locus_tag="VFQ61_03130"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="catalyzes the conversion of guanosine
                     5'-triphosphate,3'-diphosphate (pppGpp) to guanosine
                     5'-diphosphate,3'-diphosphate (ppGpp); pppGpp and ppGpp
                     control the stringent response during amino acid
                     starvation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     pyrophosphatase"
                     /protein_id="HET9953468.1"
                     /translation="MTQTRRSSGRAPEGSECHQRHSAGRCALHLLAIPSSPPWGYGVA
                     VHQEFERTVSGVDLGSNSFHMIVARLEGDGRVHVLDRLREPVRLAEGLNERRELTGKA
                     AERALGALERFAQRLRGLPEQAVRAVGTNTLRQAKNGQAFLERAESALERPIEIISGH
                     EEARLIYLGVAHSTSDDSGRHLVVDIGGGSTELVIGERFEALALHSLYMGCVSFTSRF
                     FPHGVLQRDGFRRAELAAALEVSSLLEEFRGVGWQRVTGSSGTALAIHDVIRAQRWGE
                     GITLDGLRKVKQALIEQGSVGKLSLRGLEPDRAAVFAAGVAILKGVFESLGIAQMQVS
                     QGALREGLVYELLGRVDHEDVQERTIRTLAERYKVDTHQAARVGATACDLFEQARPSL
                     EIDQAFGEQYLGWAARLHEIGLSIAYSGYQKHSAYLIEHGEMPGFSREQQLVLAAIVR
                     THRRKIAREPFAALSKQHKKPAFLLAVIFRLAVRLHRSRGANPVPTLRLQATKSDFTL
                     TFPSGFLEEHALTRADLDEECEQLKSVGVSLNVR"
     gene            3575..3907
                     /locus_tag="VFQ61_03135"
     CDS             3575..3907
                     /locus_tag="VFQ61_03135"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HET9953469.1"
                     /translation="MFALYSARDLMEAELLAHRLAEAGIEYVIRNRYLQGAIGELPAS
                     LRPEVCVLRAGDVARAEAIRDEFERNRAAPLTTEELTCPACGETSPANFELCWRCQSP
                     LFGERASP"
     gene            complement(4033..6252)
                     /locus_tag="VFQ61_03140"
     CDS             complement(4033..6252)
                     /locus_tag="VFQ61_03140"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9953470.1"
                     /translation="MDGRKRLLGLRGSLGLLMTVSACGTGHPEPGGPLGGNGTGADAG
                     SDPWGHWTGTGGASGAGRGGATIVAGTTNAGGASSAGGSSSGGASTTGTPPANLPGDR
                     VFAEHQILEFHLSLSEADWQELEEHGDREEYLPASATVTGDGFSSREFASVGLRHKGA
                     YSLHHCWDNFGGVRSHTGDCQKLSYKIKFDEYESGSRLDGLKRINLHASSGDPTKLRE
                     LLAYDTFRSFGVDASRARPAKVYINGIYQGVFIAVEDLDGRYTKAHYPEGPNGNLYKE
                     VWPRVEAADSDFVAALETNEDEADVSDMRRFAEAIAQASATTFREDMARWIDLDAVLR
                     YIAVDRAIKNWDGIMAFYSPISPHNFFWYHEEDAQDRFHLIPWDLDNTFWPFDPYMHP
                     QDWATAAPIPDWNSKPAHCEPRPVWTPDSGVYVTPPRCDPFLDLLAQTSYERFTELGQ
                     ELLQGPLKFTTANLKIANWMTELEPLVIDDPTLDYAVWLQEISAFPFYLQRSTIDFQA
                     FLAQGLIEETPPPDEPSEEELNAPTEDVGFQFPGITNFEFASAAPSAPAGLWTAGSPL
                     ASYVASWNTNAPLSGNADLRFDFTLRREPGAYDEWVNLGMYSLLSAEFDVRGQQALLL
                     RLSSDRRRNIRVRVTSPAYDDTFGGAWSEFGIDLSVDASNQLYAIPLSALSYAEWAKA
                     AWAAGQGWTTSDEEARDLVLSRCNGVALAPSATFDANGELASEEETGYVQVDNIYFR"
     gene            6742..7749
                     /locus_tag="VFQ61_03145"
     CDS             6742..7749
                     /locus_tag="VFQ61_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9953471.1"
                     /translation="MSQPGGAAQHAAGQHGIENGGPGTADIRRFDAVLSGYAYPFPVS
                     SFPVMHRGQPLSMRYMDVSPQQPNGKVVLLLHGKNFVAASWGQTMRALLDRGYRVIAP
                     DQIGFGKSEKPADYPFSFQGLAENTRALLASLGVQQSAVVGHSMGGMLATRYALMFPE
                     HTSKLVLVNPIGLEDWKTVVPHRSVELLYADELAQTRDTLRAYQEKSYYAGVWKPEYD
                     PYLEPLAGFTQDPDYPKVAWCSALTQEMIFTQPVVYELTALRVPTLLIIGQRDRTAIG
                     KARAPAKVAETLGNYPRLGRATARAIPGSKLVELDGIGHLPQVEAFPRFIAELESFLK
                     K"
     gene            7932..>8117
                     /locus_tag="VFQ61_03150"
     CDS             7932..>8117
                     /locus_tag="VFQ61_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET9953472.1"
                     /translation="MLLARLVSTSASVSATRSRLEKVALLAECIRQLSPEEKVLGIAY
                     LCGELPQGKLGVGFASLP"
BASE COUNT         1509 a         2438 c         2602 g         1568 t
ORIGIN      
        1 ttaacctgga gaggagagca aaccacgatg aaaagggtag tttggttttt gagtctgctg
       61 tggggggtcg gagcgctcgg ggccgttggc tgtgggaaca tcgacacgcc actcggggag
      121 ctgccgccag tggatggggc caagtgcctg ctccaagggg taacggcggc ggcgccggat
      181 ggctgcaata cctgctcgtg cggctcggac gggctctggg actgcaccga gaaggactgt
      241 tccggggtgt gcatgcccgg ggcaaccaag ctgagcgagg acggctgcaa cgcttgttcc
      301 tgctcggcag acggcagcgg ttgggagtgc acgacgaaag actgtgcgca gacctgcgct
      361 ccaggcgcgc gaaagccggc gggggatggc tgcaatagtt gcgtgtgtag ccctgatggc
      421 acgagctggg actgtacgtt gaagtcctgc acgatgcagt gcaagcccgg tgacaccaag
      481 ctagcggagg atgggtgcaa tgattgcttc tgcgatgaaa gcgggagctg ggcgtgtacg
      541 ttagtggatt gcgcgccgga gtgccgtccg ggagcgatga agatcgtgga ggatggctgc
      601 aacgagtgcc tgtgtagcag cgacggtcgt tggtcctgta cggacaaggc gtgtatgacg
      661 gagtgccgtc cgggagcgat gaagatcgcg gaggacggct gcaacgagtg cctctgtagc
      721 agcgacggtc gttggtcctg tacggacaag gcgtgtatga cggaatgccg tccgggcgag
      781 atgaagagca gcggcgacgg ctgcaacagc tgcttctgtg accagagcgg tcattgggcg
      841 tgcaccgcgc gggattgtgc tcccgagtgt accgaagggg agtcaaagcc ggcaggtgac
      901 ggctgcaaca cgtgctcgtg cgtctctggg cgctggagct gcacgatgag agcctgtatg
      961 ccgcaatgca agcccggcga gaaaaccttc gacggttgct cgtcttgcgc ctgtgacgac
     1021 gcaggtcgct ggttgtgcac ggccaactac tgtggcgagt gtccgcctcc cgccatggtc
     1081 ccgtctgcac aatgtgcagg gggcgcggtg tttgcgaaga atccagagtt cggcacgtgt
     1141 tgcgcgtacg atgcggcttg tcaggctccc agtgactgga agacgttctc cactcaatcc
     1201 gcatgtgctt acgctgagta aacgagcggg atagaccggg aacgcggttc cgtgcggcga
     1261 ggcggttcgt ggctgccgat caggcttctt gatcgacgac gcgcacgcct cgccggctct
     1321 tctcgagttc agtggcagcc cgcaccctct catagagctc gaccacatcg gtggggatgg
     1381 cttgcagaca cagctccggg gtcgttttat cgaatgtttt gagcaacagg ttgcccgtcc
     1441 cgagcaagcg ttgcgcgaac ggacgttcgg cggtgtagtc ggcgattcga tacaagtcga
     1501 tttgttcgag ccgtttggac aatacccccg tctcgacaac gatcctcctc gttgtgacgc
     1561 ggtagtgcac tccccgtcgt tgccaccacc gaggtaggag atacagccct aaagtcagga
     1621 tcgtgagcag cagcgcaccg agactcggaa tcagggccgg gcgaccttcg aacaatagct
     1681 cctcgcccga ttcacccgac cgcccatcgc ccgaggcctg cgttgacgct ccagaaccag
     1741 cgctcatgcg cagacgagta gcccagcctc gccgtcggcg ccagcggtgg gcgaaaacgg
     1801 ccgttcgaac gcagctcgaa agtgcaacga gcttggccgc gaagtgtcgc cgtaagcgtg
     1861 acgcagactc ggcgctcctc ggggcgagcc cctgaaggct cggagtgcca ccagcggcac
     1921 agcgcggggc gctgcgcgct gcacctgctc gctattccgt cgagcccgcc gtggggttat
     1981 ggagtagcgg tgcaccagga attcgagaga accgtctccg gtgtggatct cggctccaac
     2041 agctttcaca tgatcgtggc ccggctagaa ggagatggcc gagttcacgt gctcgatcgc
     2101 ttgcgcgaac ccgtgcgtct cgccgagggg ctgaacgaac gccgagagct gaccggcaaa
     2161 gcagcggaac gggcgctggg agccctcgag cgctttgcgc agcgtctgcg cgggttgcct
     2221 gagcaagccg tgcgggccgt cggcacgaac acgttgcgac aagccaagaa cggtcaagcg
     2281 ttcctcgaac gcgcggagtc cgcgctcgag cgacccatag aaatcatctc cggtcacgaa
     2341 gaggccaggc tgatttactt gggggtagcc cattccacct cggacgactc ggggcggcat
     2401 ctggtggtgg acatcggcgg cggcagcacc gaactcgtga tcggcgagcg cttcgaggca
     2461 ttggcgctgc acagcctgta catgggttgc gtgagcttca cctcacgttt ctttcctcac
     2521 ggagtcttgc agcgagatgg ctttcgtcgc gccgagttgg cggccgctct cgaggtgagc
     2581 tcgctgctcg aggagtttcg aggtgtcggc tggcagcgtg tgaccggctc ttccggaacc
     2641 gcgctggcga tccacgatgt cattcgcgcg cagcgttggg gagaaggcat tacgctcgac
     2701 ggcttacgga aggtcaagca ggcgctgatt gaacagggga gcgtcggcaa gctttctttg
     2761 cgtggtctgg agcccgatcg cgcggccgta ttcgcggctg gcgtcgcgat cctgaaggga
     2821 gtgttcgaaa gccttgggat tgcccagatg caggtctcac agggggcctt gcgcgaaggc
     2881 ctggtttacg agctgctcgg gcgcgtcgat cacgaggacg tacaagaacg aaccattcgt
     2941 accttggccg agcgctacaa ggtggacacg caccaggccg cgcgggtggg ggcgactgcc
     3001 tgtgatctgt tcgagcaagc ccgcccgagt ctcgagattg atcaagcctt cggcgagcaa
     3061 tatctagggt gggccgcgcg tctgcatgaa atcggcttga gcatcgccta ctcgggttat
     3121 cagaagcaca gcgcctacct catcgaacat ggggagatgc cgggattctc gcgcgagcag
     3181 cagctggtgt tggcggccat cgtgcgcacg caccgccgaa agattgctcg cgagccattc
     3241 gcggccttga gcaaacaaca caagaagccc gccttcctct tggccgtgat tttccggctc
     3301 gcggtgcgcc tgcatcgcag tcgtggcgcc aacccagtgc cgaccttgcg cttgcaagcc
     3361 accaagagtg acttcactct aaccttcccg agcgggtttc tcgaggagca cgctctgaca
     3421 cgcgccgact tggacgaaga gtgcgaacag ctgaaatcag tcggcgtgag cctgaacgtg
     3481 cgctagcgat gaacccagcc agccccctcg acatgctcgg ccgtaacacg ctcggccgta
     3541 acacgctcgg ccgtaagcgg agtagagtgc agtcatgttt gccctctact ccgcgcgtga
     3601 cctgatggag gcggagctcc tcgcacatcg cttggcggaa gccggtatcg agtacgtgat
     3661 tcgaaaccgc tacctacaag gtgcgatcgg ggagctaccc gcctcactcc gcccagaagt
     3721 atgtgtcttg cgagccgggg acgtggcgcg cgccgaagcc attcgagacg aattcgagcg
     3781 caatcgtgca gcgcccctga cgaccgaaga gctgacttgc ccggcctgcg gcgagacgag
     3841 cccggcgaac ttcgagctgt gttggcgttg tcagagcccg ctctttggcg agcgggcatc
     3901 cccgtgacct actccaacgc gaagacgcag acctcgtccg tgccgccgac gccctgccgg
     3961 atggcctttc cggcgaaata gatccggtca tcgagcgccg ccgcttgcag gagggattgg
     4021 ctcacttcgc ggtcatctga agtaaatatt gtcgacctgc acgtagcccg tctcttcttc
     4081 gctggcgagt tcaccgttgg cgtcgaaggt tgcgctcggc gcgagcgcga cgccgttgca
     4141 acgcgataag accaagtccc gcgcttcctc gtcggatgtg gtccagcctt gccccgcggc
     4201 ccacgcggct ttggcccatt ctgcgtaaga gagcgcgctg agcggaatgg catacagctg
     4261 attgctcgca tcgacactca ggtcaatgcc gaattcactc caggctccgc cgaatgtgtc
     4321 gtcgtatgcc gggctcgtca cccgcacgcg gatgtttcgg cgccggtcac tcgagagccg
     4381 tagcaacaaa gcttgttgac cccgtacatc gaattccgcg ctcagcaggc tgtacattcc
     4441 caagttgacc cattcatcgt acgctccggg ttctcgccgt aacgtgaagt cgaagcgcag
     4501 gtccgcgttg ccagacaacg gcgcattggt attccaggac gccacgtagc tggccaaggg
     4561 gctgcccgcc gtccacagac ccgcgggagc gctgggcgcc gcgctcgcga actcgaagtt
     4621 ggtaatccct gggaattgaa atccaacgtc ttcggtcggg gcattgagct cttcttcact
     4681 cggctcgtca ggtggcggtg tttcctcgat caaaccctgc gccaagaagg cctgaaaatc
     4741 tatggtgctg cgctgaagat agaatgggaa tgcgctgatt tcctgcagcc agactgcgta
     4801 gtcgagcgtc ggatcgtcta tcaccagcgg ctcgagttct gtcatccagt tcgcgatctt
     4861 gagattggcc gtcgtgaact tgagtggccc ctgcagtagc tcttggccga gctcggtgaa
     4921 gcgctcgtag ctcgtctgcg cgagcaagtc gaggaaagga tcgcagcgcg gcggagtcac
     4981 gtagacgccg ctgtccgggg tccaaaccgg gcgcggttca cagtgagcag gcttggagtt
     5041 ccaatccgga atgggcgcgg cggtcgccca atcctgaggg tgcatgtacg gatcgaatgg
     5101 ccagaatgtg ttgtcgaggt cccaagggat caggtggaag cgatcctgtg cgtcttcctc
     5161 gtgataccag aagaaattgt gcggtgagat cggcgaatag aacgccatga tgccgtccca
     5221 attcttgatc gctcgatcga ccgcgatgta ccggagcacg gcatccaaat cgatccagcg
     5281 cgccatgtcc tcgcggaagg tggtcgcgct cgcttgggcg attgcttccg cgaagcgtcg
     5341 catgtcgctg acatcggctt cgtcttcatt cgtctcgagc gcggccacga agtcgctgtc
     5401 ggccgcttcc acgcgaggcc aaacttcttt gtacaggttg ccgttcgggc cctcgggata
     5461 gtgggctttg gtatagcggc cgtccagatc ctcgaccgcg atgaacacgc cttgatagat
     5521 gccgttgatg taaactttgg caggacgggc gcgagaggcg tccaccccga acgagcggaa
     5581 cgtgtcgtag gcgaggagtt cgcggagctt ggtcggatct ccagaggagg cgtggagatt
     5641 gattcgcttc agtccgtcga ggcgtgaacc agactcgtac tcgtcgaatt tgatcttgta
     5701 ggacagtttc tggcagtcac cggtgtggct ccgtacccct ccaaaattat cccagcaatg
     5761 gtgtagcgag taagcgcctt tgtggcgtaa gccaacgctc gcgaactcgc gactcgaaaa
     5821 tccgtcgccg gtcaccgtgg cactcgccgg taaatactcc tctcgatcgc cgtgctcctc
     5881 gagctcttgc cagtctgcct cgctcaagct gaggtgaaac tccaagatct ggtgttcggc
     5941 gaatactcga tcaccgggga ggttcgcggg aggagtgcct gtcgtggagg caccaccaga
     6001 actactccca ccagcgctcg acgcaccgcc ggcgttcgtg gtgcccgcga cgatggttgc
     6061 accacctcga ccggcgccgg aagccccgcc cgtccctgtc caatggcccc acgggtccga
     6121 gccggcgtca gcgccagtgc cattacctcc aagcgggcca cccggctccg gatggcccgt
     6181 gccgcacgcg ctgacggtca tcaacaagcc caacgaacca cgcaatccca gcaatcgttt
     6241 tcgcccatcc atgctcctac gatatccgat ctgccgcgcg aacggtgcgc cggatgaaca
     6301 tcgcggatgg aaagcgactg cactgcccgt cgggccacag tgctggcggc tctcgccggt
     6361 ttccaccgag gcgtcacccc ggcctcgcac acgcactcgc gcaccaagct tggattccgc
     6421 gatattcagg ccgggatcag tcgcttcgct ctcctacggt ctccggaatt ctactgggag
     6481 ggctcgaacg caacacgctt ggtgatgggg gaagagcctc atctcgcgtg ctgcagctgc
     6541 gtgctattga cccgtgtgct cttccgaaag ccctgcgccg cgccctggca cacgtccttc
     6601 attgcatcca ccatcatcgg ctggtgtgcc ggtctcgttg cctgcagcga agccgcgaca
     6661 ccatccccgg cggcgccggt tcaagcttcg accgaacctg cagttccgtc cgttacgtca
     6721 gcgaacccac cggcactccc agtgagtcag ccgggcggtg ccgcgcagca cgcagctggt
     6781 caacacggca tagagaacgg aggaccaggt accgcagaca tccgtcgatt tgacgcggtg
     6841 ctcagcggct acgcttaccc atttccagtc agtagcttcc cggtcatgca tcgggggcag
     6901 cccttgtcta tgcgttacat ggacgtgagc ccgcaacagc caaatggcaa ggtcgttttg
     6961 ctgctacacg gcaagaactt cgtcgcggcg agctggggcc aaaccatgcg cgcgttgctc
     7021 gatcgaggct accgtgtgat cgctccggat cagattggct tcggcaagtc tgaaaagccg
     7081 gctgactacc cctttagctt ccagggtctc gccgagaata cgcgcgcgct tttggcaagt
     7141 ttgggggttc aacagagcgc ggtcgtgggt cactcgatgg gcgggatgct ggcaacgcgc
     7201 tatgcattga tgtttccaga acacacgtcc aagttggttc tagtcaaccc gattgggctg
     7261 gaagactgga aaaccgtggt tccccatcgc agcgtagagc tgctgtatgc ggacgagttg
     7321 gcgcaaaccc gcgatacgtt gcgcgcttat caagagaaga gttactacgc tggagtttgg
     7381 aagcccgaat acgaccctta cctcgagcct ctcgcgggtt ttacccaaga tcccgactat
     7441 cccaaggtgg cttggtgctc cgcgctcact caggaaatga tcttcaccca accggtggta
     7501 tacgagctca cggccctccg cgttccgacg ttgctgatta ttggacaacg tgaccgaacc
     7561 gcgatcggca aagcgcgcgc tccagcgaaa gtggcggaga cgctcgggaa ctatccgcgc
     7621 ctggggcgtg ccaccgcgcg agctatccca ggctcgaagc tcgtggagct ggacggcatt
     7681 ggccatttgc cccaagtgga agcgttcccg cgcttcatcg cggaactcga aagcttcctc
     7741 aaaaagtagg tcgaggccga actcgagtta acagagagct gccggtgccc aacgtcgcgc
     7801 gaggagcggc ttgggaacgg gcgcggcctc gtggcgaggc ggcgctaacg agcactccag
     7861 ttgcgctcgc gtttacgctt acgtggcgag cccttagcgc accaagcgac gagtgataac
     7921 ctggcgggac gatgttgctg gcccgcttgg tctctacttc cgcgagcgtc tccgccaccc
     7981 gttctcgcct ggagaaggtt gccctattgg ccgagtgcat tcggcagttg agtcccgagg
     8041 aaaaggtgtt ggggatcgct tatctctgcg gggagcttcc gcaaggtaag cttggggtag
     8101 gtttcgcatc attgccc
//
LOCUS       DASTKU010000091         6813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1131889, whole genome shotgun sequence.
ACCESSION   DASTKU010000091 DASTKU010000000
VERSION     DASTKU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131889"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VFQ61_03155"
     CDS             complement(<1..1364)
                     /locus_tag="VFQ61_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9953473.1"
                     /translation="MNSLRRLLIRLIDLQYRRPWLIVALALLTMIPSALAIRKLEIRT
                     AFSELLPDDKPSVVELRRVNQRLPSASTLTVVAEAQQMELLKRFVDELTPKLRALPKT
                     EVAAVDPGPREAQEFFEAHKLLYVPLADLESLHERVVEKYDREVQKRAGTDLGLDGDD
                     AESTGSAPLLDANDLKQRIDAGFAKLLKAAPGQDGYYIGEGGKLAAIFVRTTLSSGDV
                     KAFQLTEKIGRMIDSMGYAQIDPSFKYAFTGNLITSAEQYRAITNDLVSIGVAGVALV
                     LMVVFVFFLRKRALFALGLSIVIGCAWSLSFAWLSIGHLNTATGFLISIIAGNGINAM
                     VIWMARYLEARRSQQQGVREALETATLDTFEATLAVVAVSMVSYGALMTTGFRGFRHF
                     GVIGGAGMLLCWLSAYGVLPAVLVLSERLSPLDVRASLRDRLAGLYGRPFAWLAKRYS
                     APVA"
     gene            complement(1501..1644)
                     /locus_tag="VFQ61_03160"
     CDS             complement(1501..1644)
                     /locus_tag="VFQ61_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953474.1"
                     /translation="MNSKDDDIEARDAESAAAPRRTWLDTVLWVVLPMALVVALAVRF
                     WPR"
     gene            complement(1909..2313)
                     /locus_tag="VFQ61_03165"
     CDS             complement(1909..2313)
                     /locus_tag="VFQ61_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953475.1"
                     /translation="MRNTRRVSTPSPLQLLLRSALLFVLILGWVSCSRLPTPYGGTQQ
                     VPEITLLLAHSTKAALVHERNDRAAAVASDGKRSPPSRPSTTFTAAFGTLWPKSPPLR
                     ESAPLVLQGSEIPRVIAIHRRIPRLGDEPPWC"
     gene            complement(2366..4111)
                     /gene="ybaL"
                     /locus_tag="VFQ61_03170"
     CDS             complement(2366..4111)
                     /gene="ybaL"
                     /locus_tag="VFQ61_03170"
                     /inference="COORDINATES: protein motif:HMM:NF007950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HET9953476.1"
                     /translation="MPHETALLATLAAGLGLAFLFGLGAARLKLPPILGYLVAGMAIG
                     PFTPGFVADVGLAGQLSEIGVILLMFGVGLHFSIDDLLAVRRIAVPGAIGQIAVATAI
                     GCVLAHAWGWTWGAGLVFGLCLSVASTVVLLRALEDRGALESADGRIAVGWLIVEDLV
                     TVLALVLLPAVAPMLRGEGDLELSALGGTLALTLVKVGAFVGLMLVVGRRVVPWVLGR
                     VVRTGSRELFTLAVLAIALGIAVGVAGLFGVSFALGAFFAGVVVSESDFSHEAATNAL
                     PLQDAFAVLFFVSVGMLFDPAVLIRQPLQVIAAVAVVLLGKSLAALAIVLAYRYSVHT
                     ALTIAASLAQIGEFSFILVALAVSLGLIPQEAQSLVVAVAIVSITVHPLMFRLVDPVD
                     AWIRARRGVSEVLERPAGELTELPPAFAGTELNGHVVVVGSGRVGAPVAEELARHRVP
                     YVIVEQSRERAEQLREQGLPVLYGDASRPEVLTNARLERAKLVLVTAPDAYQARATLS
                     LGKRLNPEVEVVVRTHSDDERDWLEHHGASYAMMSERELAVSMTRHALRRYGVEHDMV
                     QVAQRVLRPVSKPDT"
     gene            4524..5363
                     /locus_tag="VFQ61_03175"
     CDS             4524..5363
                     /locus_tag="VFQ61_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953477.1"
                     /translation="MNRAWMMVIPLLSFLGGCVPTKEAKTAGQIGCTPEEITISNADS
                     HFGTVQSGATWVAECQGHTFVCSQINQSGKDKSFLDAMLASEQVTCHEAPESLEAERN
                     RRGREVASVERANRPPSKAPTGAAGFAFGETQEEVAHHCEAAGQTWRSDTRACSGPAA
                     PLGIPANVAVEFCEGRVCSIALEHFPHANWSRASVSLKANLESKYGAAQRSDGSVPAQ
                     CRSERAFTRCLQSRRVSLRYVWQWAGGESIEMNVGKRNETDLAAIRLFYRRPADSANL
                     SAL"
     gene            complement(5758..6729)
                     /locus_tag="VFQ61_03180"
                     /pseudo
     CDS             complement(5758..6729)
                     /locus_tag="VFQ61_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT         1387 a         2244 c         2070 g         1112 t
ORIGIN      
        1 atggccaccg gcgccgagta acgcttggcg agccaagcga acggtcgccc atagagccct
       61 gccaaccgat cgcgcaggga cgctcgaacg tcgagtggac tcaagcgctc gctcaacacc
      121 agcaccgcgg gcagcacgcc atatgccgac agccaacaca ggagcatgcc cgcgccgcca
      181 atcacgccga agtgccggaa cccacggaac cccgtcgtca tcagggcgcc gtaggacacc
      241 atcgacacgg cgactacggc gagcgtggct tcgaaagtgt ccagtgtggc cgtctcgagc
      301 gcttcacgta cgccttgctg ctggctgcga cgggcctcta agtagcgcgc catccagatc
      361 accatcgcgt tgatgccatt gcccgcgatg atgctgatga gaaaaccggt cgcggtgttc
      421 aggtggccga tactcaacca ggcgaacgac aaggaccaag cacagccgat cacgatcgag
      481 agtccgagcg caaagagcgc ccgtttacgc aagaagaaga cgaacacgac catcagcacc
      541 agggccactc ccgcgacacc aatcgacacc aagtcattgg tgatggcccg atactgctcc
      601 gcgctagtga tcaagttgcc ggtgaaggcg tacttgaagc tcggatcgat ttgcgcataa
      661 cccatcgagt cgatcatgcg cccaatcttc tccgtcagct gaaacgcctt cacgtctccc
      721 gagctgaggg tggtgcgcac gaatatggca gcaagcttgc ctccttcgcc gatgtaatag
      781 ccgtcttgtc ccggcgcggc tttcaggagc ttcgcaaagc ctgcgtcgat tcgctgcttc
      841 aagtcgttgg cgtcgagcaa cggtgcggac cctgtgctct ctgcgtcgtc gccgtcgagt
      901 cctagatccg tccctgctct cttctgcact tcgcgatcgt acttctcgac gacgcgttca
      961 tgcagactct ccagatccgc gagcgggaca tagagcagct tgtgcgcttc gaagaactcc
     1021 tgcgcctcgc gggggcccgg atcgaccgcg gccacctcgg tcttcggcaa agcacgcagc
     1081 tttggcgtca gttcatcgac gaagcgcttc agcaattcca tctgttgagc ttcggcgact
     1141 accgtcaggg tggatgccga cggcaagcgc tggttgacgc gccggagctc tacgacactg
     1201 ggcttgtcat ccggtaagag ttcgctgaac gcggtgcgaa tttccagctt ccggatcgcg
     1261 agcgcgctcg ggatcatcgt gagcagcgcg agcgccacga tgagccacgg cctccgatat
     1321 tggagatcga tgaggcggat caataagcga cgaagggagt tcatatgggc tgcaggtcga
     1381 aacagggcgc gtcagggagg accggcgggg ccggatccgg gaggttgggt ctcggcgagg
     1441 cgagaggtag cgcatactcg cactacctct cgccagtcga gctggttcta tcgcttgcct
     1501 tcagcgcggc cagaaccgaa ctgcgagtgc gacgaccagc gccatcggga gcaccaccca
     1561 gagcaccgta tcgagccatg ttcggcgtgg cgccgccgcc gactcggcgt cccgagcctc
     1621 aatatcatca tctttagaat tcatcacaaa gcccgtcctg agtgccccaa cgaggggtcc
     1681 gaggcacccc aatttgcggg tgcgcgcacg ccgggttcat gccgcgtgtg caccacgttt
     1741 catcgtgcgt gtgcaccgca agttccagtg cgcgtacacc ctcggccaaa ggttcttcag
     1801 atccgaagct ggccggtcac tgccttctcg atcctcgcag gcatgcgccc gtccaccgct
     1861 ttttcacggt atgcgccagc agattgaatt tctttgtgtg ggataccttc agcaccaggg
     1921 cggttcatcg ccgagccgcg gaatgcgacg atgaattgcg attactcgcg gaatttcgga
     1981 gccctgcaga accagcggcg cgctctctcg caagggcggc gacttgggcc agagggtccc
     2041 aaaagcagcc gtgaaagtgg tggagggtcg agatgggggt gagcgcttcc cgtccgaggc
     2101 gaccgcggcg gctctgtcgt tccgctcgtg gaccagcgct gccttcgtcg aatgcgcgag
     2161 cagcagcgtg atctcgggca cctgctgcgt cccgccgtac ggggtgggca agcgcgagca
     2221 actcacccat ccgagtatca acacgaacag caacgcgctg cgcagcagga gctgcagcgg
     2281 gctgggcgtg gaaactctcc gggtgtttct catcgagcgt gcgctggcat gcaagcacgc
     2341 ccgactacgc tgtgcaaccc gaggctcaag tgtcaggctt ggacacggga cggagaaccc
     2401 gctgcgcgac ttgcaccata tcgtgctcga ccccgtagcg acgcaatgca tggcgggtca
     2461 tgctgaccgc gagttctcgt tcactcatca tggcgtagct cgccccgtga tgctcgagcc
     2521 aatcacgttc atcatcactg tgcgttcgca cgaccacctc gacttcggga ttgagccttt
     2581 tgccgagcga tagcgtggct cgggcctgat aggcatcggg cgcggtcacg agcaccagct
     2641 tggcacgctc gagtcgcgcg ttggtcagca cttcgggtcg gctcgcgtca ccgtacagca
     2701 ccggtaagcc ctgctcgcgc agctgctcgg cccgttcacg gctttgctcc acgatgacat
     2761 aaggcacgcg gtgcctcgcc agctcctctg cgacgggcgc gcccactcgg cctgagccga
     2821 ccaccaccac gtgaccattc agctctgtgc ctgcgaaggc cgggggcagc tcggtcagct
     2881 cgcccgcggg gcgctccaga acctcggata caccgcgtcg cgcgcgaatc catgcatcca
     2941 ccggatctac gagccggaac atcaacggat gcaccgtgat cgagacaatg gccaccgcca
     3001 cgaccaaact ctgcgcttcc tgcggaatca gaccaaggct caccgcgagc gccaccagaa
     3061 taaaggaaaa ctcgccaatt tgcgcgaggc tcgccgcgat cgtcagcgcc gtgtgcaccg
     3121 aatagcgata cgcgagcacg atcgcgagcg cggccagtga cttgcccaaa aggaccaccg
     3181 ccaccgcggc gatcacctgc aagggttgtc gaatcagcac cgccggatcg aagagcatgc
     3241 ccactgatac gaaaaacagc acggcgaacg cgtcttggag cggcagcgcg ttggtcgcgg
     3301 cctcatgact gaagtcggac tcggacacga ccacaccggc gaagaacgca cccaaagcaa
     3361 atgagacccc aaatagtccc gcgactccga ccgcgattcc gagcgcgatc gccaacaccg
     3421 ccagcgtgaa caactcgcgc gagcccgttc gaaccacgcg accgagcacc cacggcacga
     3481 cgcggcgccc caccaccagc atcagcccta cgaatgctcc gactttgacc aacgtgagcg
     3541 cgagcgtgcc gcccagcgcg gagagctcga ggtctccctc accgcgcagc atgggagcca
     3601 ctgctggcaa cagcaccagt gcgagaaccg tcaccagatc ttcgacgatg agccagccca
     3661 ccgcgatgcg cccatccgcc gactcgaggg cccctcgatc ctctagagcg cgcagcagca
     3721 ccaccgtact cgccaccgac aagcacagcc caaacaccag ccccgcaccc cacgtccagc
     3781 cccaggcgtg ggccagcacg catccaatcg cggtcgccac tgcgatctgc ccaatcgcgc
     3841 ctgggactgc gatgcgtcgc accgccaaca agtcatcgat cgagaagtgc aggcccactc
     3901 cgaacatcag caggatcacg ccaatctccg agagttgccc ggcgagcccc acatccgcca
     3961 cgaaacccgg cgtgaacgga ccgatcgcca tccccgctac gagatagccc agaatggggg
     4021 gcagcttgag gcgcgccgcg ccgagcccga acaggaacgc gagcccgagg cctgccgcca
     4081 atgttgcgag gagcgccgtc tcgtgaggca tctcgcaaac gtaataccca gatcccaaca
     4141 gcgtccaatg cgaatgcagg tctaacctgc acgcccgcga tgggagggga ggagccaaac
     4201 cgagtaaagc tcgggcatcc gggaataagc tcgagcctga gcgatatccc gccgaagccc
     4261 ggtcgagggc gacccccgaa ctgggctcgc gagctcgcgg ttaccgcaca acgctgggcc
     4321 aacccctatg cgatcaggct cgaagtctgc ccaccgcttc cgagggcctc acgagtgacg
     4381 caccggattc acatggggcg cgagcacgcc cgtccacact cgaaacgagc gcgcgaaaca
     4441 agacacttct ttcgcgcatg gcacatcgaa tgcacaagcg ggtgagcgca gctgatgcga
     4501 aagccaatgc tggtgaagag caaatgaacc gtgcgtggat gatggtgatc ccgttactca
     4561 gtttcctggg cggatgcgta ccgaccaaag aggcaaagac ggccggccaa atcggatgta
     4621 caccggagga gataacgatc tccaacgccg acagccactt cggtaccgtt cagtccggcg
     4681 ccacctgggt tgccgagtgt cagggccata ctttcgtgtg ctcgcagatc aatcaatctg
     4741 gtaaagacaa gagctttctg gacgctatgc tcgcgtccga gcaggtgacc tgccacgaag
     4801 cccctgaatc tctcgaagcc gagcgcaacc gccgtgggcg cgaggtagcg agcgtcgagc
     4861 gcgccaatcg accgccctcg aaagcgccca ccggcgcggc cggctttgct ttcggagaaa
     4921 ctcaggaaga agtcgcacat cattgcgaag ctgcgggcca aacctggcgg agcgacacgc
     4981 gagcctgctc gggccccgcg gcccctctcg gcatccccgc caatgtagcc gtcgagttct
     5041 gcgaagggcg ggtgtgctcg attgccctcg aacacttccc gcacgctaac tggagtcgcg
     5101 cctccgtatc gctcaaagcc aacctcgaat ccaagtacgg cgctgcacaa cgaagcgacg
     5161 gaagcgtccc cgcgcagtgc cgcagcgaac gcgcattcac gcgctgcctc cagtcccgac
     5221 gagtttccct gcggtatgtc tggcagtggg cgggcggaga atccatcgag atgaacgtcg
     5281 gaaagcgcaa cgagaccgac ctcgccgcca tccgtctgtt ctatcgccgc cccgcagact
     5341 ccgcgaacct atccgccttg taacgccgcc cgccacccgg aactgctcat caagagctcc
     5401 gcggcactcc tatccacctc tcgtccgctc atcctgggcg cggaccaccg tgccgacaac
     5461 accgccgact tcaagtttcc ttgctagttg gccactctgc atacgagcgc gaccccgcgt
     5521 cccagcgccc gaaagctccg ccccttctcg gcacctcccg tccccacacc gcgccggccc
     5581 tccaattgaa ggtccgcccg agccagtagc tgcacgaggt gtgctacgcg ggccgcggag
     5641 catcgagatg agcccgtgcc ttcttcaacc aacacgcaat agacaggtac gtgcttgagc
     5701 cacgggagag cccgactcag cacaatccga gtgagcgcgg ccgaggtcga atcgcgacta
     5761 ctcggcgggg gcgggcatgt ctccgctaat ggtgccggag ggagtggggg agcgagcgcg
     5821 gagtttgcgt agaagtcggt ctcggccgtt ggccaggaga tcatagaagc tcgggatgac
     5881 aatcaaggtg aggagagtgg acgtgatgac accaccgatg acggcgcggc caaggggagc
     5941 gcggaagtcg gcgccttctc cgaatcctat cgcgacgggg agcattccgg cgatgatggc
     6001 aaggctggtc atgaggatgg gtcggaggcg aacgcctccg gcttcgacga tggcttcttc
     6061 gcgcgttttg ccctccttct cgctccactt ggcgaagtcg atgagcaaaa tggcattctt
     6121 ggcgacgatg cccatcaaca gaatcactcc gatgaggctc atgaggttga gcgtggagcc
     6181 ggtgaccagg agcgcgagcg taacaccgac gagagacagg ggaagcgagg ccatgatcgc
     6241 cagcggctcg aggaaggaac tgaattgaat gacgagcacg aagtacatca gcatcacggc
     6301 gacgccgagt gcgactagga ttcggccgaa tacctcgcgc tggtcttcgg tttctccgcc
     6361 ctgggtaacc gagtaacctg ccggcaagtt cagctgggcg gtgcgacgtt cgatgtcttg
     6421 aatgacctgg gtcagcggac gccccgcggt gttggcttcg acgatcacga cgcggtcgcg
     6481 gtcgaggtgg gtgatgatgg cgggcccgag gccgctcttc acgtgcgcta cttgggagag
     6541 cgggatgacg acgggggatt gccccggacg ctgaacgaga atgggaagag tctcgaggct
     6601 ctcacggttc tttcgcgcct cggactggag tcgaaccatg acgtcgcgcg tttcgccgct
     6661 cggatccacc caatctccgc tatccacgcc cgcaaatgcg ggcctgagag tctgcgcgac
     6721 gtcgccggcg gtaaccgcta gtgcaccgca tgtgagatgc gatcaagcat tcggcggtgg
     6781 ctgcggatga tagcggcagc gggcttggtc cag
//
LOCUS       DASTKU010000092         4144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1578168, whole genome shotgun sequence.
ACCESSION   DASTKU010000092 DASTKU010000000
VERSION     DASTKU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578168"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFQ61_03185"
     CDS             <1..649
                     /locus_tag="VFQ61_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET9953478.1"
                     /translation="DRILRSEAPKVSVRFIPNTLDDPAALRDQGSDLAVGIYGELPQE
                     MRHRRLLTDRFVCVVRKGHPAASERFTIERFIGLLHVQVAPRGRPGGYLDDVLRERGL
                     TRNVARAVPYFLSALQLVAETDYVLTISERIAKLYSGPLGLRLLEVPIKLRPYALSLV
                     WHPRVDGDAGQRFLREVFLRASKLAAGERHDSPRTRLDPSDPTSGQTRKRPRRSV"
     gene            834..1604
                     /locus_tag="VFQ61_03190"
     CDS             834..1604
                     /locus_tag="VFQ61_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET9953479.1"
                     /translation="MSLRDPNPANETNPKRVAIVIANPTVSSTTGWPVGFWWSELTHP
                     YYAFREAGYDVQIFSPAGGKCEADGMSDPRDASGYSAADLISMGFIHTPSLRALVEST
                     EPVSALDVSQFDALVVAGGQAPMFTFEGAAELHAKFAAFYEAKKVVAALCHGVALLRY
                     ARLSSGELLVQGKTVTGFANLEEDFADRAVWSMGLLPKDKHLMPWRIEDELKKLGANF
                     VHSGMWRGFAIRDGNLITGQQNFSGAETASLVIETLGR"
     gene            1799..3367
                     /locus_tag="VFQ61_03195"
     CDS             1799..3367
                     /locus_tag="VFQ61_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9953480.1"
                     /translation="MVVLGVVFGGVLAALVAVVVTAVTASVLTGSAAWWVGGVTAAGL
                     CLALPMLLDRRLTRAFRRLQPGAGSTFFQTLALVNALWLAVLVLLAPQRTRLGLEQRG
                     ARLVPLLSQRHVERIAALIPRSAAPIAIARARGASGRAAAPSPSVGTIIPPEPSAAPV
                     SVPIPVSSGIRPASDVETDSETPAGKVFRERASSVVVIHAHIAVPEDGVMSKLYERLG
                     VSYAESLGSGFVVDSAGLIVTNHHVVEGATSLRVVTQDGSHYEEVSLLRDEPKHDLAL
                     LSVSAQGLPAAPVSRAKDVSVGARAIAIGSPLGFEYTLTDGIISQRRNVDGTRFLQMQ
                     TAIAPGSSGGPLFDEHGSVIGVNTATGGAPGLSLAVHFSEVLKLLAAPRSPKVLERFV
                     PGPKLASLESEGAELAPTDRMNLREGTSLLGHVAMKCAKPLVEDARVVVKLKGLTGSP
                     SIETNLSDEARNCMDPMLTLIGMQIAFAFANLAQPPRALLLTFVDIPREGGTSGSLNY
                     RFERGEGPLLPGVP"
     gene            3597..4052
                     /locus_tag="VFQ61_03200"
     CDS             3597..4052
                     /locus_tag="VFQ61_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953481.1"
                     /translation="MKNLVFGLGVLLICLMAPARAEAKGIPVVYNTGQEVFETGPLPA
                     PFDKASELEGYKAGYLCDITGVLWSYFSVRNCKPVAYKDTSYSDDAEIVKAISATYTE
                     ADMKRGIWGHFGWMLMAGLVALGVLVWIYEAVTGKSSDDNEEASSSRQA"
BASE COUNT          734 a         1244 c         1360 g          806 t
ORIGIN      
        1 ggatcgcatc ctgcgctccg aggctccaaa agtaagcgtt cgtttcatcc cgaacaccct
       61 ggatgatccc gccgcgcttc gggaccaggg ctcggacctc gcggtcggca tttacggaga
      121 gttgccgcag gagatgcgcc atcgccggtt gcttacggac cgcttcgttt gcgtggttcg
      181 caagggtcac cccgcagcct ccgagcgctt caccatcgag agattcatcg gccttctgca
      241 cgtccaagtg gccccccggg gccgacccgg cgggtacctg gatgacgtgc tccgtgaaag
      301 agggctgaca cgcaacgtgg cccgggccgt gccctacttc ctcagcgctc tacagctggt
      361 ggccgaaacc gactatgtgt tgacgatatc ggagcgtatc gcgaagctct actcagggcc
      421 gctgggcctg cgtttgctgg aagtgccaat caagctcaga ccttacgcac tcagcctggt
      481 ctggcatcca cgggttgacg gggacgcggg gcagcgcttc ctgcgggagg tgttcttgag
      541 ggcgtcgaaa ctagcggcag gcgaacggca cgattcaccc cgaacccgtc tcgacccgag
      601 cgatccgacc tctggacaaa cccggaagcg cccgcggcgt tcggtttaga acctattgca
      661 gtagaaccgc cgcagactgg ctcctattgg ataggttttt ggcgcaggcg agacatctag
      721 ctgagttgca tcgaatgcaa ctgcagattc gatgcgattg cattgcctgc atccgaggcg
      781 cgcccgatat tcgtttcgca gggcagcgag tgccgcccaa ggagtgaagc gatatgagcc
      841 tcagagatcc caacccagcc aacgaaacca acccgaagcg ggtcgcgatc gtgatcgcga
      901 atcccacggt ctcgagtact acgggttggc cggttggctt ttggtggtca gagctaaccc
      961 atccctacta cgccttccga gaagcgggct acgacgtcca gatcttcagt cccgctggag
     1021 gcaagtgcga agccgacggt atgagtgacc cacgggacgc gagcggatac tccgcggcgg
     1081 acctaatcag tatggggttc atccatacgc cgagccttcg cgcgttagtc gagagcacgg
     1141 agcccgtctc ggcgctcgac gtcagccaat tcgatgcgct cgtggttgcg ggaggacagg
     1201 cgccgatgtt cacgttcgaa ggcgcggctg agctgcacgc gaagttcgcg gcattctacg
     1261 aggccaagaa ggtcgtggcg gcgctctgcc acggagtcgc gctgctccgt tacgcgaggc
     1321 tttcgagtgg tgagctcctc gtccaaggca agacggtaac gggattcgcg aatctcgaag
     1381 aagacttcgc cgatcgagca gtctggagca tgggcctgtt gccaaaggac aagcacctca
     1441 tgccctggcg tatcgaggac gaactcaaga agctgggtgc taatttcgtg cactccggga
     1501 tgtggagagg attcgcgatc cgcgacggca atctcatcac tggtcagcag aacttctcgg
     1561 gtgccgagac cgccagtctc gtcatcgaga cgctcggtcg ttgagccctg accctgaccg
     1621 gcgcgctggc gccggggtcg agcgaagggt gaggcgttca gacgcgtcgc cctgcggagg
     1681 ttcggtccga gtcggaggtg gctttcgctg gcgactcgcg cggaagccac ggcgcttgcg
     1741 ccgagcgcag accgcgccgt tcccgcggag caaaagacga gctacactcg cgtgcgtgat
     1801 ggttgttttg ggtgttgtgt ttgggggggt attggcggct ctagtggccg ttgtggtgac
     1861 cgccgtgacc gcctcggtgc tgaccgggag cgccgcctgg tgggtcggcg gggtgacggc
     1921 ggccgggttg tgcttggcgt tgccgatgct gctggatcgt cgactgacgc gcgccttccg
     1981 caggctgcag ccgggcgcgg gcagcacgtt cttccaaact ctggccttgg tgaacgcgct
     2041 ttggctcgct gtgctcgtgt tactggctcc tcagcgtacg cgccttgggc tcgagcagcg
     2101 cggcgcgcga ctggtgccgc tgttgagtca gcggcacgtg gaacgcatcg ccgctctcat
     2161 cccgcgcagc gccgcgccga ttgctatcgc ccgcgccaga ggcgcgagcg gacgggctgc
     2221 ggctccgagc ccgtccgtcg gtacgatcat tccgcccgag ccgtcggcgg cccccgtttc
     2281 ggtgccaatc cccgtcagtt ccggtatccg ccccgcgtcg gacgtagaga ccgattccga
     2341 gacaccggcg ggcaaggtgt ttcgagaacg cgcgagcagc gtggtcgtga ttcacgcgca
     2401 catcgccgtg cccgaggatg gagtgatgag taagctctac gagcggctcg gggtgagcta
     2461 cgcggagagc ttgggtagcg gtttcgtggt cgattcggcg gggctcatcg tgaccaatca
     2521 ccacgtggtg gagggggcga cgtcacttcg agtcgtgact caggatggct cgcactatga
     2581 agaggtcagc ctcctgcgcg acgagccaaa gcacgatctg gcgctgctct cggtatccgc
     2641 gcaaggcctg cccgccgcgc ccgtgtctcg agccaaggac gtgtcggtcg gcgcgcgcgc
     2701 gatcgcaatc ggcagcccgc tgggcttcga gtacacgctc acggacggca tcatcagtca
     2761 gcgacggaac gtggacggca cacgctttct ccagatgcag accgcgattg caccgggttc
     2821 gagtggcggg ccgctgttcg acgagcatgg atcggtcatc ggggtgaaca ccgcgaccgg
     2881 aggcgccccg ggcttgagcc tagccgtgca cttcagtgag gtcctgaagc ttctggccgc
     2941 gcctcgttca cccaaagtgc tggagcggtt cgtgcccggc ccaaagcttg cgtctctaga
     3001 aagcgaaggg gcggagctcg cgcctaccga tcggatgaat ctgcgcgagg gcacgagctt
     3061 gctcggacac gtggcgatga agtgtgcgaa gccactggtc gaggacgcgc gcgtggtcgt
     3121 caagctcaag ggcctcacgg ggagcccgag catcgaaacc aatctctcgg acgaagcgcg
     3181 caactgcatg gatcccatgc tcacactcat cggtatgcag atcgcgttcg cgttcgcgaa
     3241 cctggcccag ccgccgcgtg cgctgctact caccttcgta gacatccccc gtgagggtgg
     3301 tacgtctggt agcttgaact accgattcga gaggggtgag ggtccgctcc tacccggagt
     3361 gccctaatcc aaccggcgac tcagactggc gcttcaggtc ggccgcatca tcccgctggt
     3421 tgatgatccg gcctgatctt cggctgtgac tcggaacgag gcatccgaca gggtctgttc
     3481 cgaacgggag ccgggcacta attattaatg ctcgcgacct ttgcaggtcc gctcacgacc
     3541 atcatttctt cgaaattggg gtaggaatgc gcgctcgcca cagtactatc tccaccatga
     3601 agaatttggt gtttgggctg ggggtcctgt tgatttgtct aatggcccct gctcgcgcgg
     3661 aggctaaagg aattccggtc gtctacaaca cgggacaaga ggtgttcgag actggccctt
     3721 tgcccgcgcc tttcgataag gcgagtgaac tcgagggtta caaggccggc tacctgtgcg
     3781 acatcaccgg tgtcctttgg tcatacttct ccgtgcgcaa ctgcaagccg gtggcctaca
     3841 aagacacgtc ctattcggat gatgcagaga tcgtgaaagc gatttctgcg acgtataccg
     3901 aggccgatat gaagcgcggg atttgggggc acttcggttg gatgttgatg gctggcctag
     3961 tagccttggg cgttctcgtt tggatctacg aggcggtgac gggcaaatcg tcggacgaca
     4021 acgaagaagc ctcgtcttcg cgacaggcct agacttcgtg ctcagtcacg tctagctgcg
     4081 gttcctcgag ccgcagctag accgcactgc cgcttcacga aatgagatcg ctggcggaca
     4141 ggtt
//
LOCUS       DASTKU010000093         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1132394, whole genome shotgun sequence.
ACCESSION   DASTKU010000093 DASTKU010000000
VERSION     DASTKU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132394"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFQ61_03205"
     CDS             complement(<1..447)
                     /locus_tag="VFQ61_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953482.1"
                     /translation="MSAAESIQNGTSPAGRDSWFERVRGELRAGKLAPYVGPGLLSLG
                     SDVQVPTSYSGLAEFLGGKVSLPRRARGNAWAAAQFIESRQHRATVTALMKQAFAADV
                     AALPFHRYLVDIAPPLIVDTWYDGALRNAFAGKPDWVEIQGITRAGI"
     gene            complement(444..656)
                     /gene="nifT"
                     /locus_tag="VFQ61_03210"
     CDS             complement(444..656)
                     /gene="nifT"
                     /locus_tag="VFQ61_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HET9953483.1"
                     /translation="MKVMIRRSSTGLSAYVPKKDLEEPIVAHEHESLWGGWVRLKNGW
                     VLELPAMAPETTLPITVNARKRGEEE"
     gene            complement(653..946)
                     /locus_tag="VFQ61_03215"
     CDS             complement(653..946)
                     /locus_tag="VFQ61_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HET9953484.1"
                     /translation="MGLGKEEEIEVYGPPKFMPGAKVRSTKYVKNDGTYAGKEIGEVL
                     VKKGAVGYVRDIGMYLQIYYIYAVEFIELGTVVGMRGRELERIEDAPVARSSQ"
     gene            1297..1677
                     /locus_tag="VFQ61_03220"
     CDS             1297..1677
                     /locus_tag="VFQ61_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET9953485.1"
                     /translation="MAITAESAVTPSIQVTPGALKFIQRMVRFGGAGADAGFRLEVTP
                     GGCSGMSSQFSVEAAPKSGDRVVSLAGGLRLFLPPFSYALLEGVTIDFLESATEARLT
                     FIDPNAKSCGCSSSGPETKLTKLS"
     gene            1766..>2336
                     /locus_tag="VFQ61_03225"
     CDS             1766..>2336
                     /locus_tag="VFQ61_03225"
                     /inference="COORDINATES: protein motif:HMM:NF017312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe4S-binding leucine-rich repeat protein"
                     /protein_id="HET9953486.1"
                     /translation="MSLALPMHPEQGPPAVDWQGNPIRCEDCPCHRAEARAQCEPRHA
                     CVQDRYALRISRFFTWNPRLADNWLTHPYFEVRAIAARHANLFRISVLLNDPDETVRA
                     SVAMRVPQTLLRRLVHDPHREVRVRVAQRLVPRSLVAMLKDPDYYVRVWVARRIPHTF
                     LARMSEDTESEVRMEVAERLSPGALVMLAR"
BASE COUNT          393 a          781 c          707 g          455 t
ORIGIN      
        1 gatgcccgcg cgggtgatgc cttggatttc tacccaatcg ggcttgcccg cgaacgcatt
       61 gcgcaacgcg ccgtcgtacc aggtgtcgac gatgagcggc ggcgcgatgt cgacgaggta
      121 gcgatggaat ggcagggccg cgacatcggc cgcaaaggcc tgcttcatca gcgccgtcac
      181 ggtcgcgcgg tgctgccggc tctcgatgaa ctgcgcggct gcccaggcgt ttccgcgggc
      241 acgccgcggc aagctcacct tgcctcccaa gaactccgcg aggccggagt agctcgtcgg
      301 gacctgtacg tcggacccga gcgagagcaa accaggcccc acgtagggag ccaacttccc
      361 ggcgcgaagc tcgccgcgca cgcgctcgaa ccaagagtcg cgaccggcgg gcgaagtgcc
      421 attctgaatg gactctgcgg cgctcattct tcttctcccc gctttcgcgc gttcacggtg
      481 atcggcaacg tggtctcggg cgccatcgcg ggcagctcca atacccagcc attcttcagg
      541 cgaacccacc caccccaaag ggactcgtgc tcgtgcgcca cgatgggctc ctcgagatcc
      601 ttctttggca cgtacgcgga gagacccgtg ctcgacctac gaatcatgac cttcattgtg
      661 aactcctcgc aacgggcgcg tcttcgatac gctcgagttc cctcccgcgc attcccacta
      721 ccgtgccgag ctcgatgaac tcgactgcgt agatgtaata gatctgcaag tacatcccta
      781 tgtcgcgcac gtagccaact gcacccttct tgaccagtac ctcgccgatc tccttgccgg
      841 cgtaggttcc gtcgtttttg acgtacttgg tcgaacgcac cttcgcgccc ggcatgaact
      901 tcggaggccc gtagacttcg atttcctctt cctttccgag tcccattgtc gtttcctttc
      961 gtcgttagct cggattgcgg ggtgccgtgc tcggccagcc gcaactttga ggatcgaagt
     1021 ggcattccga tccggtgcac ttcctggccg aaggcgaaag caaccgatgt gccacgcggg
     1081 gtcactcgaa aatcggaggc tcctagggct ctgccgcgcg ccccagacgc gtcggctttg
     1141 tcgcgaagtc gacaggttgt cgtagcgatt cgagttgtcg gtcggcttgt ctgcgattcc
     1201 cgtgtgcttc ccgaagccaa gggtcgtggc acgaatttgg cttacgcccc atcgcgcgct
     1261 ggccggcgcc cgcgccttcg ccccgagttt aaaagcatgg caatcacagc tgaatccgcc
     1321 gttacgccct cgatccaggt taccccaggc gcgctcaagt tcatccagcg catggtccgc
     1381 ttcggcggcg cgggagccga cgcaggcttc cgactcgaag tcactcccgg gggctgctcg
     1441 ggcatgtcgt cgcagttcag cgtcgaggcg gccccgaaga gcggtgaccg cgtcgtttcg
     1501 ctggctgggg gactgcgctt gttcttaccg ccgttctcct acgcgttgct cgagggggtg
     1561 acgatcgact ttctcgagag cgcaaccgaa gcacgcctga ccttcatcga ccccaatgcc
     1621 aagagctgcg ggtgtagctc gagcgggccg gaaacaaagc tgacgaagct cagctgaatc
     1681 gactacggaa gccacggtca ctgtcgcgtc gtggtaccgc ttgcgttacc actgagcgtg
     1741 gcgcccatgg cgaaggagcc caagcatgag cctcgctctc ccgatgcacc ctgagcaggg
     1801 tccccccgcg gtcgattggc agggaaatcc gatccgctgc gaagattgcc catgccaccg
     1861 agctgaagca cgagcgcaat gcgagccgcg acacgcctgc gtccaggacc gctatgcact
     1921 tcggatctcc cggtttttca cctggaaccc tcggctcgca gacaactggc tcacccaccc
     1981 gtacttcgag gttcgcgcga ttgcggcgcg acacgcgaac ctgtttcgca tctcggtgtt
     2041 gctgaacgac cccgatgaga ccgtgcgagc ttcggtcgcg atgcgcgtac cgcagactct
     2101 gctccgacgc ttggttcacg accctcaccg agaagtgcgc gttcgcgtgg cgcagcgcct
     2161 ggtgccgcgc tcgctggtcg ccatgctgaa ggacccggac tactacgttc gggtctgggt
     2221 ggcgcggcgc attcctcaca ccttcctcgc gcgcatgagt gaagacaccg agtcggaggt
     2281 gaggatggaa gtggccgagc gcctgagccc tggcgcgctc gtcatgctcg ctcgcg
//
LOCUS       DASTKU010000094        27880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_909394, whole genome shotgun sequence.
ACCESSION   DASTKU010000094 DASTKU010000000
VERSION     DASTKU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 27880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27880
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_909394"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFQ61_03230"
     CDS             complement(<1..678)
                     /locus_tag="VFQ61_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953487.1"
                     /translation="MNEALLTLILTTGFASAGCLAYAVASAPTTQPDLLGIRGLKRIR
                     AVSGNTTFARFEPLLRWLGTQLKRVLSDKTCAQLDRQLALAGDFLGLRAEEFVAMCGL
                     FGLAGLLFGLGMAHAFAQPPEMVLGTAVIALFLPYTRLTSMQEERRRRATHGLPPVVD
                     LLVLCLSAGLDFPGAVRHVVAKASNPSDAFIEELGFLLQELNVGKTRKLALTQLADRL
                     PTDSMREF"
     gene            complement(675..1541)
                     /locus_tag="VFQ61_03235"
     CDS             complement(675..1541)
                     /locus_tag="VFQ61_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9953488.1"
                     /translation="MSLPQDLGALKWATCAGLLLLVLVIVYAVAINPESPPHRAYLRY
                     IGHLDLKLGRMLLPTRARWIVVGQLFVLELVALACAALGENWLLVGIPIALLAPVLYV
                     EQLRRARVARIEKQVDSFMLTLANALRATPSIGNALSYTHHLVAEPIRSELGICLHEV
                     RVGNSVEQALLNMGARVQCPTLDSALQALLIGRQVGGDLTKILETTAVTLREMARLQG
                     VLRTKTAESKVQILVIALMPVGLVYGLSRMTPGFFDPLFADLVGKTLLVVAVVLWLGA
                     MLLARKILAVEL"
     gene            complement(1538..3196)
                     /locus_tag="VFQ61_03240"
     CDS             complement(1538..3196)
                     /locus_tag="VFQ61_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9953489.1"
                     /translation="MELEIVVESMNGERRVERRHVSGALSVGRHATCTVHVEDNHISR
                     EHALLHPLAGGIRVQDVSSNGTLVGERLLRRESVDVPYGTPILLGRHTLFVSPFNPLP
                     LPAAPPPENDVLPVADPELIEEAPAATSLPDTGALRREIHRRLLQHLDLSCWDPKRVD
                     DVALRPRVLAALHRIVECLGAELPDGIQREALVGEVADEALGLGPLERLIADNSVTEI
                     MVIDANTIFVERAGKLVRSPTKFTDDDRVRAVIERIVTPLGRRIDESSPLVDARLLDG
                     SRVNAVIKPLAIKGSCITIRKFSKVPFTLENLCSFGALTESMGRFLTRSVVAKRNIVI
                     SGGTGSGKTTLLNILSAAIPDDERIVTIEDAAELQLRQTHVVSLETRPANMEGTGSYT
                     IRDLVKNALRMRPDRIVVGECRGGEALDMLQAMNTGHDGSLTTTHANSPREALARLET
                     LSLMAGVDLPSRAIREQIAKSVHLVVQQSRLSDGSRKVTAISELCGIDPDGEFELHPI
                     YRFVRTGTGSNGKVLGEFLATGRLPSFLGEFIVRGLIEPGEPYL"
     gene            complement(3235..4614)
                     /locus_tag="VFQ61_03245"
     CDS             complement(3235..4614)
                     /locus_tag="VFQ61_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HET9953490.1"
                     /translation="MKAWTSSVALASLVSLLSTVTSAQPNEKPPERAARGKEHVIGED
                     FPPVVRQQQEEINISVGENRTLPATNVRSYSEATPGIAEVRLTPDSSQFIIVGQQPGS
                     TTLLLIKKDNTQVTYTINVFARSLERVESELVQLLEGTPGMRVRRVGPRFFIEGGVST
                     EPELKRIERIAAQFPGQVESLVVLGGAAAERRINVRIDFYFVQFDRGKSYQVGVGYPG
                     RFGGGALHADFDFLTSAFTSATASVVNQPLPGLDLAATQGWAKILKHSTVIASNGCEA
                     KFSSGGEQNFIVSSGLSTSLTQLPFGTNVAVLPRFDPASRELEVRVNAEVSDLTPSVA
                     SDVPGRSVSKVDTLVSLKLGQSIVLSGIRTRTERHSVSGLPLLSEIPVLGILFGSHGN
                     AKEEVEGAVIVVPSVVESVPKGAREVLDEALTRYQGFSGDMTGIDAWDKVPPAPAPAP
                     SAIVAPVSR"
     gene            complement(4611..5420)
                     /gene="cpaB"
                     /locus_tag="VFQ61_03250"
     CDS             complement(4611..5420)
                     /gene="cpaB"
                     /locus_tag="VFQ61_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HET9953491.1"
                     /translation="MNKTALAIAAFLAATCTALLLVYLNRIERETSGGERVRVLALVK
                     SVERGQMITDDMLATRAVPMAYVEDRSVKAPERPKVLGLQAAHALSPQQLLLWTDLAI
                     TTEERELSSLVQPGKRGVTVQAAGANEGPGNALIRPGDYVDVIVTSVDAKDLKDQSAS
                     VLLQRVLVLAVGQETGQIAGSADLRGRESAIAERLLTLSLNLSEAQLLALALEKGRLS
                     VAVRNVGDPAIQGDVPDVNATALFTGRVRVEPKPVPRAASGGPVAIGGFKR"
     gene            complement(5417..6421)
                     /locus_tag="VFQ61_03255"
     CDS             complement(5417..6421)
                     /locus_tag="VFQ61_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953492.1"
                     /translation="MFTAPGSMPLVAAEPQRISFWRGALRFLAYAILINLAFLVVAGS
                     VAWASVERTTAEVSASLLTDIPPGLLDTPRAFQINGQRVWVSSKLTRAEVPEVLEAFS
                     AHCRDRSASLSHQLEQLPRGGASRAIRFNERLSQQLLENSGAAGHVACLAPRPRADST
                     SLVQSVRRFAANGDLSVFGDVRYAVARRVPETGDTHVVALWTEGPFSVAALLSHSSDA
                     PGSDSLAAPRPANSRRLLSASGETTSYAVRIYDSPHAQRVALDDYTQQLARLGFERLE
                     LPVGSVSDSMSSSTRSFHKEGTAVLVIATPLATGHSNIALVELESPGRVEANGARELP
                     "
     gene            complement(6433..6903)
                     /locus_tag="VFQ61_03260"
     CDS             complement(6433..6903)
                     /locus_tag="VFQ61_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953493.1"
                     /translation="MTRTYSPRASFHGAIVSLLSRKRRQRGSTILPALLLLTLFGGLA
                     AFGVRTRQSYLAHASEVAEARTAAWLAATRGCRRQPPVDRKLLSRGFERGVPRAPRAF
                     YPTRTAELGLDRVHLQASSEFGTSIACNEPSGAADSELHEAEQHFRRHLLPPLR"
     gene            complement(6954..8759)
                     /locus_tag="VFQ61_03265"
     CDS             complement(6954..8759)
                     /locus_tag="VFQ61_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953494.1"
                     /translation="MTPPSLRKPVVAARRVLRSETQGDILILGIILGGILASAIYAFV
                     GLSSTLAAREASQNAADAAAFENAIWHARSMNLMASLNVLMVASVATLALWRLAVSAV
                     AFAGLTNLVVGMSEPSTAQQDEAVAIAEPLAEMLAADEGVAARVASIVTSLNAAQVAV
                     AAYAPVWALQGADSGTKRNLAHVAASASLSLLPNQSESVEHDLKACLATREVALKTAG
                     GSAERAVRSALRDAKQSTLTVQQAASAVAGFRASTTRLGAPFSLPAQRDAYSVFCSTP
                     DADWHDSSRRLLGALTSVARAAVIDLPLGAKQQALQRLGVEGHERDALARISEVSGFE
                     AGRVSGALCSPSGPAVEVWTEAAWRILARTVGHPRTPAAVREKARKLYAESHFQPDLP
                     PSDLLSLNPIDNLDRARAVWARWSETLESSSGEFECGRPAKVWNPAHNGSPYFLSTTL
                     TPASESLESASPSGPSVLTEGFWAHAEMYFDCVDQEWSRCAPNALWQPLWRARLRRVQ
                     SLSDMKVRDETGHTVSPAFPPEAVMIAGLRRVASTGGLHPSGPPAPLKAFLPFPERQR
                     VERDDALVADRNGAEGSTPLRRYLAEHAGENRMIH"
     gene            complement(8784..9308)
                     /locus_tag="VFQ61_03270"
     CDS             complement(8784..9308)
                     /locus_tag="VFQ61_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953495.1"
                     /translation="MVQLIPELGPALVPAPRGRGSCRPSGTEGAVLIEYLVVFWPVLL
                     LGMTVWQQTEIWMGELVVQRAATAAARAAAVVLPDDPALYGGTPVNAYVGRRRADIEL
                     AAALAVSGGDWFEQVPEVRVERSSSGDTVTATVKARFECKLGLFNLACQRYRSLERSA
                     QFPYQGAHYRYSRP"
     gene            complement(9296..9844)
                     /locus_tag="VFQ61_03275"
     CDS             complement(9296..9844)
                     /locus_tag="VFQ61_03275"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET9953496.1"
                     /translation="MSAMDPLFVIPSLVTAIAAIIDWRTGHIPNRLVFWGLVAGLAAR
                     FAVAAPQSERSLGLLNSAAEIGLGLLVCAAVPLVLFRMGAMGGGDVKLLAVLGACAGP
                     SVGMRIQVCAFMVGALLAAVMLARTGQLSRLLRNVGILLLNPFLPRARRREIEPAMLT
                     SLRFGPAICAATLWVGAETWFS"
     gene            complement(9930..10169)
                     /locus_tag="VFQ61_03280"
     CDS             complement(9930..10169)
                     /locus_tag="VFQ61_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953497.1"
                     /translation="MNSHIDTQVKSSNIVSVSKAVTLKKSGWLRRLLGDTRGAATMEY
                     AAIVGIVVIAGIAVLNVYKTKLGEAKTTAGSIQLK"
     gene            complement(10533..11219)
                     /locus_tag="VFQ61_03285"
     CDS             complement(10533..11219)
                     /locus_tag="VFQ61_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953498.1"
                     /translation="MTHAVSRFRSGFLGVSAVGWALSAALGCAREPVMNEYPVAIQVD
                     ANPDEPLAGVGIWSGSQKMGVTGPEGRAVTVLEGLEGQVFSLRVACPEGHREATQPLA
                     IALHTVADGDPAPLYQARCEPLQRSVVVAVRAERGPNLPLLYLGREVARTDSAGSAHV
                     LLKGASEDSIELTLDTSGSPELRPRSPSTRFEVPGNDDLLVFNQAFTLPPAPKAAPKA
                     RPTGIVNIKR"
     gene            11543..12919
                     /locus_tag="VFQ61_03290"
     CDS             11543..12919
                     /locus_tag="VFQ61_03290"
                     /inference="COORDINATES: protein motif:HMM:NF018018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9953499.1"
                     /translation="MGLLAAVGIPLLGVYREKLGGAAAEGANVQLSGDSKGLQVESAP
                     GPASPPKTAEGKKNYGDVLGKIVDFANDLIFGDRAPSADTGGIPKPGTDATAVRTWWN
                     GLPESERQRLIQQDPAAVGQLDGVPAAARDEANRILLARAPSELKAQQEALQAELRRE
                     LASPSANMSRVNHLHAEIERVQRNIQGVEALNDRLSHTGGEAKGQALLLGFDTRGEGH
                     AIVASGDPDHAGNVVTYVPGTTAKLAGIGGDQLRSDRMANRANEIDPASHTVGVTWLG
                     YDAPQSIPSAASDRYAKEGSEALSDFQAGLRATHEGPPSHNTVIGHSYGSTVVGVAAH
                     DHGLSADEMVFVGSPGVGVDHAKDLGIDAQHVWSSHAPNDPIQYTEVGSATGPLIGTP
                     PPSRMPFGINPSSSEFGARTFTSQSGGHSGYWDTTAAGSGEYTASTSLDNIAHIVVGQ
                     DEAVTARR"
     gene            12980..13579
                     /locus_tag="VFQ61_03295"
     CDS             12980..13579
                     /locus_tag="VFQ61_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953500.1"
                     /translation="MPIKDLIRAALLVLRRNWRVLLGQSACLLAAELALEIALFLSLG
                     PVAGFVRVLMAPFVAGVLLSAAHSAAKGERPKLRTAVAHAARAWPALLSVAAATYAGL
                     LVFGLGVVATSTLFLFSPLCVMLGKGPREALRISLELVAQRPLQMCLLNLVLGAAYYG
                     SYLVPFVGPLLAPVVSALLLAQTFERLSAAHSQLAPSHV"
     gene            13572..14993
                     /locus_tag="VFQ61_03300"
     CDS             13572..14993
                     /locus_tag="VFQ61_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953501.1"
                     /translation="MSERQPSRQMRNRSSRHPRNQRGTTILEYCALIGLVVLAGIVVL
                     RVLADHLRGAAEVEGLTLSAPSGAAPSGCNTPGNCAADVGATQTSLAGPARRNKEEPS
                     LWSKVAGGARSAWDFGWAMGEARIELGRAAAGKVQDFTDWVYGGINKLEDATGGFPPV
                     QQLGGLLKAGIGTAGGIVQVALNPVDAVQGLVKLNNFLNPTNNLSLALWDTLTSDVSY
                     QDALAKRFADNLKLGEQLIEPYKKDWDAGRPIDAVTHFFGDAILSVAGPKGVRVPHVP
                     AGAGGAAARDAGAAAGAASREAGAPGKAIPPGEGKVFREDTRPYDSDGKTPGTRKSYI
                     DEDGNIQPANPDGTTTPTQHVGGSEPSKSSSPYTSFKEEPGKGKAFGDHTTEVDVARL
                     KADIDSGKIKDVEIIDHDALMKLHDERVAAAQQRYDASPTPKNAERLARSQRDRANSA
                     RDGEMLIKGTIPSEYVTPAGGKP"
     gene            15052..15807
                     /locus_tag="VFQ61_03305"
     CDS             15052..15807
                     /locus_tag="VFQ61_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953502.1"
                     /translation="MTDSAMPSKIEQDHLLYEDETGEVVGLHEVSLLDPVPSERIPRV
                     QLLLLHPDIFIVYQAVLVLTAWGDDKGLNKLEELVDRRIHNEFDFAPHRSYGYDNVYD
                     EFAGALDIRRLVRTDDGAQVCRVYEKLLGLYGPCGFEDRLKYALLNCDCTMLVTPIDL
                     AITRALEWHRIYLASQLLPPLARFSPQRALERVGAFSNLGPMTPNPQVNVAEALRWIS
                     PEESRPRLEKLERHPDQVVADEARASLAKLDLR"
     gene            15853..17079
                     /locus_tag="VFQ61_03310"
     CDS             15853..17079
                     /locus_tag="VFQ61_03310"
                     /inference="COORDINATES: protein motif:HMM:NF026945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 44 domain-containing
                     protein"
                     /protein_id="HET9953503.1"
                     /translation="MCAVSVTADDAHAERARSLPSYRVLPMSPYQKEGVCVKNHSPLR
                     NKHSRRSRCRGAAILEYVGVVGIVVTAAIPVLAIYREKLLGSAGATFSLTTGSDGTET
                     GQGSSDLPLPPAYGPQDQAGIAKAAHDQMVADGKRYDDILKVKPGFQTTLLKTLPGGS
                     MPTREELSAADAIVRKYEAELRQHQGDPNFNMDDFMKRLGQELEPMAREHRNDFTWWY
                     YMVKTDSVFDLKNRKTDPNAEQLWDQPWDFSSAGVPKKASTGDTAGNWLYGYLGAEVF
                     GAGALGQSTLIGGAGAAQAAEDKGEAGKRLLQTGWIDNPGDAEKIHAGIEGYMDVHGP
                     AYVFLPPGRPPGLAVPGPSNRDAQLEWELAKAGVADAGKGIVRTGKEVGELVDKGVQH
                     YVVDPLRDWAEYTTRF"
     gene            17354..18895
                     /locus_tag="VFQ61_03315"
     CDS             17354..18895
                     /locus_tag="VFQ61_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953504.1"
                     /translation="MPRGLLPALLVPCRRSSTPLAWALTTWTISIACTVETGGHVFSG
                     AAAGAGAATGEGAAAGERVGAGAAAGAGGEGSNSTSGLAGSDDRGVDAGMPREPLTGG
                     AGGAHEYGGTSSLAEAGHDSHPDASGGRDQAGATGAEGGAIDAAGGQFNPTGGVTNDT
                     GGTGACDDLATDSENCGRCGHSCGGGDCRAGVCQPMVVVGNLGTVNGLAIDRDEVFFT
                     QGPTGTLTAGSDTASVLACPSNGCVLAPRQIAGGLSRASSAFVVGDSLAVLGSEAAPY
                     PLTALMVVSKSGTNKNYLTPNAGKGMAGLGTADVAINGSDLFFLRVGPLATGGSPAKL
                     VNVCRGVASGKCTEQVTLGARAVNPPFAADSQRIYFFEAGGLVTCPILSGCDSTTSLV
                     ASAGAPDKMKIWGDNIYLLNARKISSCPLSGCSEPASVRAQFGPDMIDFAIDDNAVFW
                     LEDSNVQTCPLPDCPGGPRRLAKDQHAPSSIAVHNGFVYWVDAGMPKDGSFEEGTAAI
                     LRVAL"
     gene            19021..19269
                     /locus_tag="VFQ61_03320"
     CDS             19021..19269
                     /locus_tag="VFQ61_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET9953505.1"
                     /translation="MQNSPALSVLDAIFLMSAETLPITRSVLARRLGLTGSALQQHLL
                     ELERRGFIDGRRLRLTLPGLALAVSMRRRRAQPTRAAA"
     gene            19956..20408
                     /locus_tag="VFQ61_03325"
     CDS             19956..20408
                     /locus_tag="VFQ61_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953506.1"
                     /translation="MPINCPPDATSYDFLGTAWIADLGSIPDAHWIWAPGVHGGDISE
                     LVKFTFVREFQLGAAPSGYITLGVDNYARVLVNGILVGELGSIQNPIFESFNQLTTFD
                     LTPALKAGMNTVSIFAANGVTDIAPNCSYTCNPAGLVFGGTLTSVTTR"
     gene            complement(20429..21457)
                     /locus_tag="VFQ61_03330"
     CDS             complement(20429..21457)
                     /locus_tag="VFQ61_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953507.1"
                     /translation="MKNRIWMSAGLGHSAMAVLGACSLIVDASVPGDVGGTAGKGGAI
                     NSAGQGGSGARGGGPNHQSGAGGDGGTLETGGSAGADLGGGGGAAGEAGTPASGGIST
                     AGNTAAGGAAVGGTPQGGTSSAGGKPPSTGGTTNKGGGGAGGSSGANHECSKPCEALQ
                     ACSAGQCVNVLSKASLRLESTCAGPGSTEYLCQAACPLPGSQSLQVAGASSVSYDVVV
                     EISGLVELTAIPDGTLDADHFWLERTAAFDSGSFDSWGLKVAGAYYYLNAVAKEDDAH
                     TPLIIEPYRKTLRVAGGTRLELFLSSSDCEQLTTCDPSCRSVQIDGRDIDTQFVEIRF
                     VSVTPVVS"
     gene            complement(21537..22559)
                     /locus_tag="VFQ61_03335"
     CDS             complement(21537..22559)
                     /locus_tag="VFQ61_03335"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9953508.1"
                     /translation="MTRIGYSCAAALMIASISAGTRTSFAADPASVTPVSPDQEAEAG
                     KHYNRAVELYTKGAYSEALAELIQTRALNHSYKVLFNIAQVRGVMQDAAGALQAYREY
                     LAEGGAEINAQRREDVQKEIRDLEARVATLIVETDLAGAQVLIDSTPAGVTPLPAPLP
                     INPGTHEVEVKHPEYPPQTRRVAFEKSRAERVAFTLAPPAPPKAVARLETSPTAQGKA
                     STDVAPSDPRTTAKWIGWVGTGVLVTGAAVTGILALSANGSLAKLRDDPNSDHAAMQS
                     KSDTTKTYAAVCDGFLIATAVAGGVSLWLTLSSPSSGEHAATTKKTPTLSVGAGPSAV
                     QLRGTF"
     gene            complement(22596..24662)
                     /locus_tag="VFQ61_03340"
     CDS             complement(22596..24662)
                     /locus_tag="VFQ61_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9953509.1"
                     /translation="MLNTACLEVDWGSPQVPESARSSLGDARRADAEGKSSPRGSVPS
                     SQPLGVLSDTSWLAQGALYVLRSANQPGFVNASIDDQDFSERAAVPRDDRYGDAHEQE
                     ELAADPGVPSITRMSGQYHTLSKYRLVARLGSGGMAEVYLALAAGPSGFKKLHVLKVL
                     RSDIAAHEQPEFLQMFQEEGRLAARLNHPHIIQSYEVGVEGDQHFIAMEFLEGQALSR
                     LQERSWPSRDPSGLGLELFVISEVLEGLEYAHNLTDYGGTPLHIVHRDVSPQNVFVTY
                     TGHAKLLDFGIAKTWESSKTQAGIVKGKVPYMAPEQVRGGPLDHRVDLFAVGVMLWES
                     AAARRMHGDSNVYEILRRMGDGAIPSLREAAPSISEELERIVVRALATEPARRYPDAH
                     SFRRDLLEVPELRGVSAREVGARLEALFAEERSELNRVIREAMRDDTEAAIFVAEKAP
                     VRLPELGHASDTSSIGSLSTTAPTTTPVIQTTPPPPMPAHGSAIPVSRGVRRQSVWLA
                     GGALGLSVAAVVLWMANRDPRPEAPDIHAVNPAGSAVGTVPPVAQREPTVPAMPADST
                     SVVSTPPASATSLPQREAAASPEIRVRISAKPPTASLLLDGKPLGGNPYQGARTRDDK
                     THVLTVLAPGYQSRSIPLSFERDLDVPVVLERSTPSPSPTPRKTGFDVDFRTLNPTRS
                     IGSSSR"
     gene            complement(24919..26196)
                     /locus_tag="VFQ61_03345"
     CDS             complement(24919..26196)
                     /locus_tag="VFQ61_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET9953510.1"
                     /translation="MSNTKGARAHQRLSTFAGIAIWAVGCSFIVDTTVPNRAASTGGG
                     ESGGDRGNAGTTSEFGGAAGSENAGAAGRGEPQNGSGGTQSGGAHAGGASSSRAGATS
                     GGGSTASGGTAELGGMNSGAAGDADGGEVKLCFCCEGVVDGRLANWLQSGEVQCAGTW
                     NDASAAVQEQQYTIQPESEYGSWQKLLENAIGGIFENETWAQAANGEFDARWERTVGN
                     IEKAWKTRDQSLLNVRFAHEFNLFDSQWRVTGSDAENFKQAWRRFHGIFKSHLPNASL
                     VWCPNDGTSGTLDLDVRNAYPGDAYVDIIAIDTYNQWPWVNDAQAFDKKLSGEYADGA
                     PLGAESWRRFAEAHGKPFAIGEWASNGDPDSEGGGGDSPDYIERFHSWLVTHGGTGAG
                     QIKYAVYFNNYKQFRVFPETIQPLAAEKVRELF"
     gene            complement(26450..27766)
                     /locus_tag="VFQ61_03350"
     CDS             complement(26450..27766)
                     /locus_tag="VFQ61_03350"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9953511.1"
                     /translation="MTDETLAKALPILVSYSKIKVSVTDGPDAGTTREIVSSTLRIGS
                     ASDNDLVLADNTVSRHHCALEPVTGGVRIRDEGSTNGILVGNMRVVDATLSGSVQLQL
                     GNTRILVEPLTETVSREQTVAERFGDLLGRSPRMRELFADLARIAPTDVTLLIEGETG
                     CGKELVAESVHGASKRFEGPLVVFDCSAVAPTLIESELFGQERGAYTGAVARPGLFEQ
                     ADGGTVFLDELGELPKELQPKLLRVLERREVRRLGGQRTVTVDVRVISATNRNLAAEV
                     QRGNFREDLYFRLATAHVRVPPLRDRMEDLPLLVDHFLARAQSNLRVSDIPPPVWNMF
                     KTHRWPGNVRELWNAVQRFLITPERVFAADVAHAGGALPAASPLPATGPLPPLREARR
                     DASDAFERAYIEALLARTSGNMKAASAMSDVSRQMIQKLMRKHGLG"
BASE COUNT         5333 a         8852 c         8628 g         5067 t
ORIGIN      
        1 gaactcccgc atcgaatccg tgggcaaacg atcggcgagc tgagtcagag ccagctttcg
       61 cgtcttgccg acgttcagct cttggagaag aaagccgagc tcctcgatga atgcgtcact
      121 cgggttgctg gctttcgcca ccacatgccg aacggcaccg gggaagtcca atccggctga
      181 taagcaaaga accaagagat ccacgaccgg aggtaggcca tgcgtggccc tgcgacggcg
      241 ttcttcctgc atggaggtca gtcgcgtata gggcaaaaac agggcgatca cggcggtccc
      301 cagcaccatc tcgggtggtt gagcgaaggc atgcgccatt ccaaggccga acagcagacc
      361 cgctagtcca aacagcccgc acatggcgac gaactcttcc gcgcgcaacc ccaagaagtc
      421 accggcaagc gctagttgcc gatcgagctg cgcgcaagtc ttgtcgctga gcacgcgctt
      481 gagttgagtt ccgagccaac gcagtagtgg ctcgaagcgc gcgaaggtcg tgtttccgga
      541 taccgcacga atccgcttga ggccccgaat cccgagtaga tcgggctgcg tggtcggggc
      601 gctcgccacc gcgtaagcga gacatccggc cgacgcgaaa cccgtcgtca ggattaaagt
      661 gagcaaagcc tcgttcatag ttccaccgcc agaatcttgc gtgcgagcag catggctcct
      721 agccaaagca ccaccgccac cacgagcaac gtcttgccca ctagatccgc gaagagcgga
      781 tcgaagaagc ccggtgtcat tcggctcaaa ccgtagacca atccaaccgg catcaacgca
      841 atcaccagga tctgcacctt gctctcggcg gtcttggtcc gcagcacccc ctgcaggcgg
      901 gccatttctc ttagcgtgac cgcggtggtt tctaggattt tcgtgaggtc accacccacc
      961 tgccttccga tcagcagggc ttgcagtgcc gagtcgagcg tcgggcactg cacacgtgcc
     1021 cccatgttga gcaacgcctg ctccaccgag ttcccaacgc gcacctcatg caggcagatg
     1081 ccgagttcac tccgtattgg ctccgccact agatggtgcg tgtaactcag cgcgttgccg
     1141 atactcgggg tggcccggag cgcgttggcc aacgtgagca tgaagctatc cacttgcttc
     1201 tcgatgcggg caacccgagc ccgtcgcagc tgctcgacgt acaagacggg ggccaacagg
     1261 gcgatcggta tgcccacgag caaccaattt tcaccgagcg ccgcgcaagc cagcgccacg
     1321 agctcgagga cgaacagttg acccaccaca atccagcgcg cgcgggtagg cagaagcatc
     1381 cgaccgagct tcagatcgag atgcccgata taacgcaggt aggcgcggtg tgggggcgac
     1441 tcgggattga tggccacggc atagacaatc accaggacca gcagaagcag acccgcgcag
     1501 gtggcccatt tcagggcccc gaggtcttga gggaggctca taggtacggc tccccgggct
     1561 cgatcaaccc tcgcacgatg aactcaccga ggaagctcgg caatcgcccg gtggccagaa
     1621 actcgcccaa gaccttgccg ttcgagcccg tcccggttcg gacgaagcgg tagatcggat
     1681 gcagctcgaa ctctccgtca gggtcgatgc cgcacaattc ggagatggcc gtcacctttc
     1741 ggcttccgtc cgagagacga gattgctgca cgacgaggtg cacgctcttg gcaatctgct
     1801 ctcggatcgc tcgggacggc agatcgacgc cggccatcag cgacagggtc tcgaggcgcg
     1861 cgagggcttc acgcggcgag ttagcgtggg tcgtggtgag cgaaccgtcg tggcccgtat
     1921 tcatcgcctg cagcatgtcc agcgcctcgc caccgcggca ctcccccacg acaatgcggt
     1981 ccggccgcat gcgcagcgca ttcttgacca agtcgcggat ggtgtaactt cccgtgccct
     2041 ccatgttcgc cgggcgtgtc tcgagggaga cgacgtgggt ctggcgaagc tgcagctccg
     2101 ccgcatcctc gatggtgacg atacgttcgt cgtcgggaat ggctgcagac aggatgttga
     2161 gcaaggtggt cttgccgctg ccggtcccgc cggaaatcac gatgtttcgc ttggccacga
     2221 cgctgcgggt gaggaatcga cccatgctct cggtcagcgc tccgaaggag cacaggttct
     2281 cgagcgtgaa cggcaccttg ctgaacttgc ggattgtgat gcaagagccc ttgatagcca
     2341 ggggtttgat gaccgcgttg acccgcgagc catccaacaa tcgcgcgtcg accaacggag
     2401 aggattcatc gatgcggcgc ccgagaggcg tcacgatgcg ctcaatcacg gcgcgcacgc
     2461 ggtcatcgtc cgtgaacttc gtgggactgc gcacgagctt gccagcgcgc tcgacgaaaa
     2521 tggtgttggc gtcgatgacc atgatctcgg tcaccgagtt atcggcgatc aagcgctcga
     2581 gcggcccgag cccgagagct tcgtccgcca cctcccccac gagcgcttct cgctggatgc
     2641 cgtccggcaa ctccgctccc aggcattcca cgatccgatg caaggccgcc agcacgcgcg
     2701 ggcgtagggc cacgtcgtcg acgcgcttcg gatcccagca ggagagatcg agatgctgca
     2761 acagccgacg gtgaatctcg cgacgcaagg cgcccgtgtc gggcaagctc gtggcggccg
     2821 gggcttcctc gatgagctcg gggtcggcga ccggcaggac gtcgttttcg ggtggaggtg
     2881 ccgccggtaa cggaaggggg ttgaaaggag acacgaacag cgtgtgtctt ccgagcagga
     2941 tgggagttcc gtacggcaca tcgacgctct cacgtcgcaa caggcgctcg ccgacgagag
     3001 tgccattgct ggagacgtcc tggacgcgga ttccacccgc tagtgggtgc agcagcgcgt
     3061 gttcgcgcga gatgtggttg tcctccacgt gaactgtgca agtcgcgtgg cgacccacgg
     3121 agagtgcccc gctcacgtgt cgacgttcca cccgtcgctc gccgttcatg ctctcgacga
     3181 caatctcgag ttccatcgca atctccaaag tcgcgttttc acctggaacc gggctcagcg
     3241 ggacacgggc gcaacgatcg cgctgggggc cggggcggga gcggggggca ccttgtccca
     3301 agcgtctatt ccggtcatgt ccccggagaa gccttgatag cgggtcaacg cctcatcgag
     3361 cacctcgcgc gcgcccttcg gaacggactc gacgacgctc ggcacgacga tcaccgcgcc
     3421 ctcgacttct tccttggcgt tgccatggct gccgaacaag atcccgagca cggggatctc
     3481 gctgagcaga gggagaccac tcaccgagtg gcgttccgta cgcgtgcgaa tgccggagag
     3541 cacgatcgac tgcccgagct tcaaagaaac gagcgtatcc actttcgaca cactcctacc
     3601 cggcacgtcg ctcgccacgc tcggcgtcaa atcggagacc tccgcgttga cacggacctc
     3661 gagctcacgg ctcgcgggat cgaagcgcgg cagcaccgcg acgttggttc cgaaaggaag
     3721 ctgtgtcaga gaggtgctga gcccgctgct cacgatgaag ttctgttccc caccgctcga
     3781 gaacttggct tcacagccgt tcgaggcgat gacggtggag tgcttgagga tcttcgccca
     3841 accttgcgtg gcggcgagat cgagcccagg cagcggctga ttcacgacgg aggcggtagc
     3901 gctcgtgaag gcgctcgtca gaaagtcgaa gtccgcgtgc agagcgcccc cgccgaagcg
     3961 gcccgggtaa cccacgccaa cttggtagct ctttccacga tcgaattgca cgaagtagaa
     4021 gtcgatacgc acattgatgc gtcgctccgc agcggcaccc ccgagcacga ccagcgactc
     4081 gacttgaccc gggaactggg ctgcgatgcg ctcgatcctc ttcagctcgg gttcagtgct
     4141 gacgccaccc tcgatgaaga agcgcggccc cacgcggcgc acccgcatgc cgggtgtgcc
     4201 ctcgagcagc tgaacgagct cgctctccac gcgctcgaga gagcgagcga agacgttgat
     4261 ggtgtaggtg acctgggtgt tgtccttctt aatcaatagg agggtcgtcg aaccgggctg
     4321 ttgcccgacg atgatgaatt ggctgctatc cggagtcagg cgcacttctg cgataccggg
     4381 ggtggcctcc gagtaactgc gcacgttggt cgcgggcaga gttcgattct cgccgaccga
     4441 gatgttgatc tcctcttgct gttgacgcac gaccgggggg aaatcttcgc cgatgacgtg
     4501 ttcctttccc cgcgccgccc gctccggagg cttttcgttg ggctgggccg acgtgaccgt
     4561 ggacaagaga ctcaccaggc tggccagagc cacactcgac gtccaggctt tcatcgcttg
     4621 aaccctccaa ttgcgaccgg gccaccgctc gccgcccggg gcacgggttt gggctccact
     4681 cgcacgcgac cggtaaacag tgccgtcgcg ttgacgtcgg ggacatcacc ttgaatagcg
     4741 gggtctccga catttcgaac tgccaccgag agccgccctt tctccagcgc cagcgctagc
     4801 agctgcgctt ccgaaaggtt caggctgagg gtcaagagcc gctcggcgat ggcagactcg
     4861 cgccctcgta aatccgcgct gccggcgatt tgtccagtct cctgtccgac cgcgagcacc
     4921 aacacgcgct gcagcaagac cgatgccgat tgatccttca ggtccttcgc gtcgacgctc
     4981 gtaacgatga cgtccacgta gtcgcccgga cggatgaggg cgttgccggg gccttcgttg
     5041 gctcccgcgg cttgtaccgt gacaccccgc ttgcccggct gaactagaga gctcagctcg
     5101 cgctcttccg tcgtaatcgc cagatccgtc cagagcagga gttgctgcgg agacagcgcg
     5161 tgggcggctt gcaaccccaa caccttgggg cgctctggtg ccttcacgga gcggtcttcc
     5221 acgtacgcca tcggaacggc acgggtggcc aacatgtcgt ccgtaatcat ctgaccacgc
     5281 tccacggatt tcacgagtgc aagcactcga acgcgctctc ctcccgacgt ctcccgctcg
     5341 attcgattca agtacacgag cagcagggcg gtgcaggtcg ccgcgagaaa tgcggcaatc
     5401 gcgagtgcgg ttttgttcat ggcagctccc gcgcgccgtt tgcttcgacg cgaccagggc
     5461 tctcgagttc cacgagggcg atattcgaat ggcccgttgc gaggggcgta gcaatcacga
     5521 gcactgcggt tccttctttg tgaaacgatc gtgtggacga agacattgag tccgaaactg
     5581 acccgacggg aagctcgagg cgctcgaaac cgagccgggc gagctgctgc gtgtagtcgt
     5641 cgagcgccac ccgctgcgcg tgcgggctat cgtagatgcg caccgcgtac gaggtggtct
     5701 cgccgctcgc cgagagcagc cggcggctgt tggcagggcg cggcgccgcg agtgaatccg
     5761 agccgggcgc gtcgctcgaa tgggagagta gcgcggcgac cgaaaaggga ccttccgtcc
     5821 agagcgcgac cacgtgcgta tccccggttt cgggcacccg gcgcgcgacg gcgtagcgga
     5881 catctccgaa gacagataaa tctccgttgg ctgcgaagcg acgcacactc tggacgaggc
     5941 tcgtggaatc agcgcgagga cgcggcgcca aacaggcgac atgcccggct gcccctgagt
     6001 tctccaggag ctgttgagag agccgctcgt tgaatcggat ggcgcgactc gcgccgcccc
     6061 ggggaagttg ttccagttga tgcgacagac tcgccgaacg atctcgacag tgcgcactga
     6121 acgcttcgag aacctccgga acctccgcgc gagtgagctt ggacgacacc caaacgcgct
     6181 gcccgttgat ctggaaagct cgaggagtgt cgagcaggcc aggaggaatg tccgtgagta
     6241 aagaggcact gacttccgcg gtcgtgcgct ccacgctggc ccacgccacc gaaccggcga
     6301 cgaccaagaa cgccaggttg atgaggattg catacgcgag aaatcgcagg gctccacgcc
     6361 agaaggaaat ccgttggggt tctgcggcaa ccagaggcat cgaaccaggc gcggtgaaca
     6421 tatcggctct cctcagcgaa ggggcggcaa caggtgacgc cggaagtgct gctcggcttc
     6481 atgcagctcg gagtcagccg ctcccgacgg ttcgttacac gcaatgctcg tgccgaactc
     6541 tgaagaagcc tgcaaatgga cccgatcgag ccccaactcg gcggtgcgcg tcggatagaa
     6601 ggctcggggc gcccgcggaa ccccgcgctc gaatccccga ctcaagagct tgcggtcaac
     6661 gggcggttgc cgccggcaac cgcgggttgc cgctaaccac gccgccgtac gcgcctcagc
     6721 cacctccgag gcgtgagcca ggtaactctg gcgcgtccgg acgccgaaag cggctaaccc
     6781 gccaaacagg gttaggagca acagcgccgg caagatcgtg ctgccacgct gtcgccgctt
     6841 gcgggacaaa agcgagacta tcgctccgtg aaaagaagcg cgcggggaat acgtccgggt
     6901 catgtcatct gcctgagtgg cgagccccga gtctcgggct cgttcgtaga gattcagtgg
     6961 atcatgcggt tttcgcccgc gtgctccgcg aggtagcgcc gcagcggagt cgagccttcg
     7021 gctccgtttc gatcggcgac cagcgcgtcg tctcgctcca cccgctgccg ctcggggaac
     7081 gggagaaaag ccttcaaagg ggcgggtggg ccgcttggat gcagtccacc cgtggacgca
     7141 actcgccgta gcccagcaat catcacggct tccggaggga aagcgggaga gacggtatga
     7201 ccggtctcat cacgcacctt catgtcggac agggattgca cccgccgcaa tcgagccctc
     7261 cagagcggtt gccacagagc attcggcgcg caacgcgacc actcttggtc gacacaatcg
     7321 aagtacatct ccgcatgggc ccagaaccct tcggtaagaa ccgatgggcc gctgggggag
     7381 gcgctctcga ggctttcgga cgccggggtg agcgtggtac tgaggaagta cgggcttccg
     7441 ttgtgggccg gattccaaac tttcgccggt cgcccacact cgaactcacc cgagctcgac
     7501 tccaaggttt cgctccaacg cgcccaaacc gcgcgcgcgc gatccagatt gtcaatcggg
     7561 ttcagcgaca gcaagtcgct cggaggcaag tcgggttgga agtgcgactc cgcatatagc
     7621 ttccgcgcct tttcgcggac ggccgccggg gttcgcgggt gacccaccgt gcgggccaag
     7681 atgcgccagg ccgcttctgt ccacacctcc accgcgggcc ccgagggtga gcacagagcg
     7741 cccgaaacac gtcccgcttc gaagccactc acttcggaga tgcgagccag cgcgtcgcgc
     7801 tcatggccct ccacgcccaa gcgttggaga gcctgttgct tcgcgccaag aggaagatcg
     7861 attaccgcgg cgcgagccac cgaggtgaga gcgccgagta gccggcgact cgagtcgtgc
     7921 cagtccgcat ccggcgtaga acagaacacg gagtaggcat cgcgttgggc agggagactg
     7981 aacggagccc cgagccgggt tgtcgacgcc cgaaaccccg caacggcgct ggcagcttgc
     8041 tgcaccgtca gtgtgctctg cttggcatcc cggagtgcgc ttctgacggc acgttcggcg
     8101 ctgccaccag ccgttttcag ggctacctcg cgggtggcaa gacaggcttt cagatcgtgc
     8161 tccacagact cgctctggtt cgggagcaga gataggctcg ctgacgcggc gacgtgcgcc
     8221 agattccgtt ttgttcccga gtccgcacct tgcagggccc aaacgggggc ataagccgcg
     8281 accgccactt gcgcggcgtt gaggctggtc acgatcgagg cgacacgtgc ggccactcct
     8341 tcatccgcgg ccagcatttc cgcgagtggc tcggcaatcg cgaccgcctc gtcctgctgt
     8401 gcggtagagg gctcgctcat gccgaccacc agattcgtca acccggcgaa cgcgaccgca
     8461 ctgacagcga gtcgccagag cgctagtgtc gccacactcg ccaccatcag gacgttcaaa
     8521 gacgccatga ggttcatgct gcgagcgtgc caaatggcgt tctcgaaagc ggccgcgtcc
     8581 gccgcattct gcgaggcttc tcgagccgca agagtgctgc tcaaaccgac aaatgcgtag
     8641 atcgcagagg caaggattcc gccaagaatg atgcccaaga tcaagatgtc gccctgcgtc
     8701 tccgaccgga ggacgcgacg tgcggcgaca acgggcttgc gcaacgaagg cggtgtcact
     8761 tacattctcc tcatgaagcc gattcacggc ctcgaatatc gatagtgcgc gccctgatag
     8821 ggaaactgtg cactgcgttc gagcgagcgg tagcgctggc aagccaagtt gaacaagccg
     8881 agtttgcact cgaagcgcgc cttgaccgtc gcggtcaccg tatctcccga agaagagcgt
     8941 tccacacgaa cctcggggac ttgctcgaac cagtccccac cggagaccgc cagggctgcg
     9001 gcaagctcga tatcggcgcg gcgtcgaccc acataagcgt tcaccggggt gccgccgtac
     9061 aacgcggggt catcgggcaa aaccactgcg gcagcacgcg ccgcggccgt ggccgcccgc
     9121 tgcacgacca actcgcccat ccaaatctcc gtctgctgcc agacggtcat cccgagcaac
     9181 aatacgggcc agaacacgac cagatactcg atcaacacgg caccttcggt accggacggt
     9241 cggcaagagc cgcgccccct gggcgcagga actagcgcag gacctagctc aggaatcagc
     9301 tgaaccacgt ttcggctcca acccagagcg tcgcggcgca aatcgcgggt ccgaaacgca
     9361 acgaggtgag catcgcgggt tcgatctccc gccggcgggc gcgcggcagg aacgggttca
     9421 gaagcagaat gcccacattc cgaagcaacc gcgacagctg acccgtgcgc gcgagcatca
     9481 cggccgccaa gagcgctccc accatgaacg cacagacctg aattcgcatc ccgaccgagg
     9541 gtccagcaca ggcgccgagc acggccagca acttcacgtc accgccgccc atagcaccca
     9601 tgcgaaagag gacgaggggc accgccgcac agacgaggag ccccagcccg atctcggcgg
     9661 ctgagtttag aagacccagc gatcgctcag actggggcgc cgcgacggcg aaccgtgcgg
     9721 caagcccagc gacgagcccc caaaaaacca agcgattggg gatgtgcccc gtccgccaat
     9781 cgatgattgc agcaatggcc gtgaccagcg aggggattac gaagagggga tccattgcag
     9841 acatggggca tcacaaacca ggtgagattg agattcagag tcctattggg gctagctcag
     9901 cgtgcaggaa ctagccccgc ctgctcgggc tacttcaatt ggatcgagcc cgccgtggtc
     9961 ttcgcttcac cgagcttggt cttgtagaca ttgagaaccg cgatgcctgc gatcacgacg
    10021 atgcccacga tggctgcata ctccatcgtt gccgcgccgc gcgtgtcgcc gagcaagcga
    10081 cgcagccaac cggacttctt gagggtaacc gccttagaga ccgaaacgat gttgctgctc
    10141 ttgacctggg tatcgatgtg gctgttcatt gttctttgct tccttcaacg gttagggata
    10201 cgaacgagct agggcttgac gctggccaac gtagctgcgc gccctggatg cgccgagagg
    10261 aacgatcaga gtgctcacga gcagaaccgt gaacgagaga atcactgcat attcgacgag
    10321 cactgcgccc tgcgtcgcgc tcggcagcga acccaagctg ggctgtccga gctctgatgc
    10381 cttgcggccc atattcgagc ttaatgcagt cggcgtgcca gctcctcagc cgcacaaacc
    10441 cagcgtattt tgaggcatgg aactggtgtg caactctcag ttgctcgaga cgattgctga
    10501 cggacaccgc gagttgcatc ctgcgcctcc agctaccgtt tgatgttgac gatcccggtg
    10561 ggtcgagcct tcggagccgc tttgggcgca ggagggaggg tgaatgcctg gttgaacacc
    10621 aagagatcat cgttaccggg cacctcgaag cgcgtactcg gactgcgtgg tctgagctct
    10681 ggactccctg aggtatcgag agtgagctcg atgctatcct cggaggcacc cttcaaaagc
    10741 acatgagccg agccggcact gtcggtacgg gccacctcgc gaccgagata taggaggggt
    10801 aaattcgggc cgcgctcggc ccgaacggcc accaccacgg agcgctgcaa gggctcgcag
    10861 cgggcctggt agagtggcgc cggatctccg tcggccaccg tatgcagtgc aatcgcaagg
    10921 ggttgcgttg cctcacggtg gccctccggg caggccaccc tcaacgagaa gacctgcccc
    10981 tcaaggcctt cgagcacggt caccgcacga ccctccgggc cagtgactcc catcttctga
    11041 gatccagacc agatcccaac gcccgcgaga ggctcgtctg gatttgcgtc cacttgaatc
    11101 gcgaccgggt actcgttcat cacgggttct cgagcgcatc caagcgccgc gctcagggcc
    11161 cagcccaccg cggaaactcc gagaaagccg gagcgaaatc gcgaaactgc gtgtgtcatg
    11221 cgcccggccc attcacgcct cgtgccggcg cgtatggaaa tgaacttgcc acgtactgcc
    11281 tctctctcga gcgcgcgggg aactctcggt tgcgacccgc agttgccatg gcaacccgtg
    11341 gttgcccccg cgaactcagg tagcgctgca ccgagggtcc ggtgcctggg cacggtgatt
    11401 gctcacgtcc cggtcatcta tgctgtctga accgacaaga atcgatctct gccgaactgg
    11461 agacggccaa ccgcggcgcc tgcgaagaag agcacgccgc gttgggacct tcggtgcgtc
    11521 catcatcgag tacgtgctcg tcgtgggcct cctcgccgcc gtgggcattc cgctgctcgg
    11581 tgtctatcgc gagaagcttg gcggcgcggc cgcggaggga gccaacgtgc agctctctgg
    11641 ggatagcaaa ggccttcagg tggagtctgc accagggccg gcgtccccac cgaaaacggc
    11701 agaagggaag aagaactacg gcgacgtact aggaaagatc gtcgatttcg cgaacgatct
    11761 gatattcggc gatcgtgcac ccagcgccga tacgggtgga attccaaagc ccggcacgga
    11821 cgcgacagcc gtgcggacct ggtggaacgg actacccgaa tcagaacgcc agcgcctgat
    11881 tcagcaggat cctgcagcag tcggacaact ggatggggtt cccgcggcgg cgcgggacga
    11941 agctaaccgc atcctgctcg cgcgcgcgcc gagcgagctc aaggcacagc aagaggcgct
    12001 ccaggccgag ctgcgccgcg agctcgccag cccctccgcc aacatgtcgc gagtcaacca
    12061 ccttcacgct gagatcgaac gggtccagcg caacattcaa ggcgtcgaag ccttgaatga
    12121 tcgcctttca cacacgggtg gtgaggcgaa gggccaggct ttgctgctcg gcttcgacac
    12181 gcgcggcgag gggcacgcca tcgtcgcgag tggcgatccc gaccacgccg gaaacgtcgt
    12241 cacgtatgtg cccggaacca cggccaagct cgccgggatc ggtggcgatc agctgcggag
    12301 cgaccgcatg gcgaaccgag ccaacgagat cgaccccgcc agtcataccg taggagttac
    12361 ttggctcggc tacgacgcgc cccagagcat cccgagcgca gcgagcgata ggtatgcaaa
    12421 agaaggcagc gaagctctga gcgatttcca ggccgggctc cgcgcaaccc acgagggccc
    12481 gccctcgcac aacaccgtca tcggccacag ctatggctcg acggtggttg gcgtcgcggc
    12541 tcacgaccac ggcctcagcg ccgatgagat ggtgttcgtc ggatcaccag gagttggagt
    12601 ggaccacgcg aaagacttag gcattgatgc tcagcacgtg tggtcgagtc acgccccgaa
    12661 cgaccccatc caatacaccg aagttggctc tgccactggc cctctaattg gcacgccacc
    12721 gccctcgcgc atgcctttcg ggatcaaccc ttctagctcc gagttcggcg cgcggacgtt
    12781 caccagtcaa tccgggggcc acagcgggta ttgggacacc accgcggcag gctccggtga
    12841 atatacagca tcgacttcgt tggataacat cgcgcatatc gtggtggggc aggacgaagc
    12901 cgtaaccgcg cggcgctaac actgcccatt tgcgtgggcc actcccacga accacccaac
    12961 gtgaaagcag gttgatacga tgccaatcaa ggacttgatt cgagcggcgt tgctcgtgtt
    13021 gcggcgcaat tggcgtgtat tgctcggcca gtccgcgtgc ctgctggcag ccgaactagc
    13081 cttggagatc gcgcttttct tgagcctcgg acccgttgcg ggcttcgtcc gcgtcctcat
    13141 ggcgccgttc gtggccggcg ttctcttgag cgcggcgcat agcgcggcaa aaggcgagcg
    13201 cccaaagcta cgcaccgcgg tggctcacgc tgctcgagcg tggcctgctc ttctgtccgt
    13261 cgcggccgcg acctatgcgg gcctactcgt ctttgggctg ggggtggtgg ccacatcgac
    13321 tttgttcctg ttctcgcccc tgtgcgtcat gttgggcaag ggaccaaggg aggcactccg
    13381 gatcagcctc gagttggttg ctcagaggcc acttcagatg tgcctactaa atctagtctt
    13441 gggagcggct tactacgggt cctatctcgt gccgttcgtc ggcccactgc tggctcctgt
    13501 cgtatcggcc ttgctgttgg cgcagacctt tgaacgcctc agcgcggccc attctcaact
    13561 ggcgccgagc catgtctgaa cgccaaccct cccggcagat gcgcaatcga agctcgcgcc
    13621 acccgcgaaa tcagcgcgga accaccatcc tcgaatactg cgccctaatc ggcttggtgg
    13681 tgcttgcagg aatcgtcgtt ttgcgcgtct tggccgacca cctcaggggt gcggcagagg
    13741 tcgaggggct cacgctctcc gcgcccagcg gtgccgcccc cagcggctgt aacactccag
    13801 ggaattgcgc cgctgatgtt ggcgccactc aaacgtcgct cgccggcccc gcccgccgaa
    13861 acaaggaaga gcccagcctc tggtcgaagg tagccggcgg agctcgctcc gcttgggact
    13921 ttggctgggc aatgggtgaa gctcgaatag agctgggccg cgccgccgcc ggaaaggttc
    13981 aagacttcac cgactgggtc tacggcggca tcaacaaact agaggacgcg acaggaggct
    14041 tcccgcccgt tcaacagttg ggcggcttgc tcaaggcagg aattggcacc gcgggcggca
    14101 tcgtccaggt agccctcaat cccgtcgacg ccgtgcaagg gctcgtcaaa ttaaacaact
    14161 tcctcaaccc gaccaacaac ttgtcccttg cgctctggga caccctcacc tccgatgtca
    14221 gctaccagga tgcgctcgcc aaacgctttg ccgacaacct gaagttgggc gagcaactca
    14281 tcgagcctta taagaaggat tgggatgcgg ggcgccccat cgatgcggtg acccacttct
    14341 ttggcgatgc catcctctcc gtcgccggcc ccaaaggcgt gcgggttccg catgttccgg
    14401 ctggtgcggg gggcgcggcg gctcgagatg ccggcgcggc cgcgggagcc gcttctcgtg
    14461 aggccggagc gcctggcaaa gccatcccac ccggtgaagg caaggtcttt cgggaagata
    14521 ctagacctta tgattccgac ggcaaaacac caggcacgcg aaagtcgtac atcgacgaag
    14581 atggcaacat tcagcctgcc aatcctgatg gcacgacgac tccgacccag cacgttgggg
    14641 gtagcgaacc gagcaagagc tccagcccgt atacgtcgtt caaggaagaa ccagggaagg
    14701 gaaaggcatt tggtgaccac accaccgaag ttgatgtcgc gcgtttgaag gcagacatcg
    14761 acagtggcaa aatcaaggac gtggaaatca tcgaccatga cgctctgatg aagctccacg
    14821 acgagcgcgt cgctgccgcg cagcaacgct atgatgctag tcccacacct aagaacgcgg
    14881 aaaggttagc gcgttcccag cgcgatcggg ctaactcggc acgtgacggc gagatgctga
    14941 tcaaaggtac catcccatcg gaatacgtga ccccggcggg tggcaagccc tgaaggtttc
    15001 gcgtgcctcg ctataattgc ggtcactcga tgaaccttgg gagagacgac gatgactgat
    15061 tccgcaatgc cctcgaagat tgagcaagat caccttctat acgaggatga gaccggtgaa
    15121 gttgtgggcc tgcacgaagt ctcgttgctc gatccagtgc catccgagcg cataccgcgc
    15181 gtccagctcc tattgctgca cccggacatc ttcatcgtgt accaggccgt tctggtactg
    15241 acggcttggg gggacgacaa agggctgaac aagctcgagg aactcgtgga tcgcagaatc
    15301 cacaacgagt tcgatttcgc tcctcatcgg agctacgggt acgacaacgt ctacgacgag
    15361 ttcgcgggcg ctttggatat tcggcggctg gtccgtaccg acgacggagc tcaggtctgt
    15421 cgcgtttacg agaagttgct cggtctatat gggccatgtg gattcgaaga tcggctcaaa
    15481 tatgccctgc tgaactgcga ttgtacgatg ctcgtcacgc ccatagacct ggccatcacc
    15541 cgcgcgctcg aatggcaccg gatctatttg gcaagccagc tgcttccacc gctcgcccgt
    15601 tttagtccgc aacgcgcttt ggaacgggtc ggcgcgtttt cgaatcttgg cccaatgacg
    15661 ccaaacccac aggttaacgt ggctgaagca cttcgttgga tctcgcccga ggaaagtcgg
    15721 ccgcgcctcg agaagctcga gcgtcacccg gaccaggtgg ttgcagacga agcaagagct
    15781 tcgttggcaa agctcgactt gcgctaagcc gtagcttacg ggcggttccc tttatacaca
    15841 ttctgccgcg acatgtgtgc cgtatccgtc accgcggacg atgcgcacgc tgagcgggca
    15901 cggagcttgc cgtcttatcg agtcttaccg atgtcgccgt atcaaaaaga aggggtatgc
    15961 gtgaagaacc actcgccact tcgcaacaag cactctcgac gatcccgatg ccggggagcc
    16021 gccattctgg agtacgtcgg ggtggtcggg atcgtcgtca cggccgccat tccggtgctg
    16081 gccatttacc gagagaagct gctcggctct gccggggcca cgtttagcct gacgactggt
    16141 agtgacggca ctgaaactgg tcaaggctct tcggatctac cactgccgcc cgcttatggc
    16201 ccccaggatc aagccgggat cgccaaagca gcgcacgatc agatggtcgc agacggcaag
    16261 aggtatgatg acatcttgaa ggtcaagccc gggttccaga ctacgctctt gaagacgctc
    16321 ccgggtggca gcatgcccac tcgagaagaa ttgtcggccg cggacgctat cgtgcgaaag
    16381 tacgaggctg agctgcggca gcaccaaggc gatcccaact tcaatatgga tgatttcatg
    16441 aaacgtctcg gtcaggagct cgagccgatg gcgcgtgaac accgcaacga cttcacttgg
    16501 tggtactaca tggtgaagac ggattcggtg ttcgacctga agaaccgaaa gacagacccg
    16561 aacgccgagc agctctggga ccagccctgg gatttcagct cagccggtgt ccccaagaaa
    16621 gcgagtaccg gggataccgc tggaaactgg ttatatggat atttgggcgc cgaggtgttt
    16681 ggtgcggggg cgctggggca gtcaacgctc atcggtggtg ctggagccgc ccaggccgca
    16741 gaggataagg gagaggccgg taagcggctg ttgcaaacag gatggatcga taatcctggt
    16801 gatgctgaga agattcacgc tggaatcgag ggctatatgg acgttcacgg gccggcctac
    16861 gtctttctac cgccggggcg gccgccggga ttggccgtgc cggggcccag caatcgggac
    16921 gcacagctcg agtgggagct ggctaaagcg ggtgttgcgg acgcgggcaa agggatcgtc
    16981 cgcaccggaa aggaagtcgg tgagctggtc gacaaggggg tacaacacta cgtggtagat
    17041 ccgctccgcg attgggccga gtacaccacc cgattttagt attgcctcag ttccgatccg
    17101 actgccgcac gagcagacgc agctctccga tttctgttta ggactcgcaa gcggactacg
    17161 gcgaacggtg ggctctaaat gacaccgtac aattccgcgt gatccgctcg ggacgattgc
    17221 acttgcgggg ctgacagcgt atcggcccag gagagctcgc gtgatatctc cgttgtagta
    17281 ctttttcctg ccgacatgaa gttccacagc ggcgggttgg tttggcgatt ggcactcgat
    17341 agattggtgg tcgatgccgc gcggactgtt gccagccctg ctcgttccat gtcgacgttc
    17401 gagtacccca ctcgcttggg ccttgaccac ctggacgatc tcgatcgctt gtacggtgga
    17461 aacgggaggc cacgtcttca gcggggccgc ggcgggagcg ggagccgcaa ctggcgaggg
    17521 agccgcagct ggcgagcgag tgggagcggg agccgcagcc ggcgcgggag gagagggttc
    17581 gaactcgacc agtggcctgg ccggcagcga cgaccgcggt gttgacgcag gtatgccccg
    17641 cgagccgctg actggcggtg ctggcggggc ccacgaatac gggggcacga gttcgctggc
    17701 ggaggccggg cacgatagcc atccggacgc cagtggcgga cgggatcagg cgggcgcgac
    17761 tggtgccgaa ggaggtgcga tcgatgctgc aggtggccag ttcaatccaa ccggaggagt
    17821 cacgaatgat accggcggga ccggagcttg tgatgacctg gcgacggaca gcgagaactg
    17881 tggccgctgc ggacatagct gcggaggtgg agattgtcgg gccggagtct gccagccaat
    17941 ggtggtagtc ggcaatctgg ggacggtgaa cggtctggcc atcgatcgag acgaagtgtt
    18001 ctttacgcag gggccaaccg gtacgctcac tgcgggaagc gacaccgcca gtgtgttggc
    18061 atgcccttcc aatggctgcg tccttgctcc caggcagatt gccggcggtc tctcacgggc
    18121 ctcgagtgct ttcgtcgtcg gcgacagtct ggccgtgctc ggctcggaag cggcgcccta
    18181 tcccctcacc gccctcatgg tggtgtcgaa gtcggggacc aacaagaact atctgacgcc
    18241 gaatgcgggc aaaggaatgg ctgggctcgg tactgctgac gtcgcaatca acgggagcga
    18301 tctgtttttc ctgcgtgtcg gtccactcgc gaccggcggt agcccggcaa aactcgtgaa
    18361 tgtatgtcga ggtgttgcgt ctggaaagtg tacggagcag gtgactttgg gtgcgcgagc
    18421 agtgaatcct ccctttgccg ccgattcgca gcgaatctac ttcttcgagg cgggtggatt
    18481 ggttacttgc ccgatactct cggggtgcga cagcacgacc tcgcttgtcg cttccgctgg
    18541 ggctcccgac aagatgaaga tctggggaga caacatctac ctgctcaacg ctcgaaaaat
    18601 cagcagttgt ccgctctccg ggtgttcgga gcctgcgagc gtccgcgcgc aatttgggcc
    18661 ggatatgatc gactttgcaa tcgacgacaa cgcggtcttc tggcttgaag acagcaacgt
    18721 gcaaacgtgc ccgctgccgg attgcccggg tggcccacgt cgacttgcca aagaccaaca
    18781 cgccccgagc tcaatcgccg tccacaatgg gttcgtctat tgggtagacg ccggtatgcc
    18841 caaagacggc tcgttcgaag agggtacggc cgccattctt cgcgtggcgc tctgagtctc
    18901 ttccccaccg tcgcggccct cgcgtcgtga cgcgcctact tgccggccgg tgacggtctc
    18961 cagcaagatt tcttctccaa aaactggatc cctgttcagg tcatttgata ctgaacatgc
    19021 atgcagaatt caccggctct ctccgtgttg gacgcgattt ttctcatgtc agccgagacg
    19081 ttaccgatca cgcgctcagt gctggcgcga cgtctgggcc tcacgggctc tgcattgcag
    19141 cagcacctcc tcgagctcga acgaaggggc ttcatcgacg gccgacggct ccgcctaacg
    19201 ctgcccggcc tggcgttggc cgtctcgatg cgcaggcgtc gtgcacagcc aacgcgcgca
    19261 gcggcgtaga actgcgcggt cgcgccgtgc aggccgcgct cacattggct tcctacgagg
    19321 gatcccgcga cagagccgga ttcgacaaag tggaaaacga cagtccccat tcggttgact
    19381 gaatagccgc cggaggtggc gagactcggc atttgcctgt cttcgtcatt tggatctcct
    19441 agctttctta tttagtctca tcgctccgct ggactgcggt gcactcaaac tccgtagcct
    19501 ccaccgaact cgctgagtcc tgccaaagaa gcgggcgaaa gcggtcccgc gagggtccag
    19561 ctctgcttgg ctttgataat cgccgttcgg caggagggga gaacgttgcg tgacgtcgga
    19621 acggagagag ggagatgaaa atggttgaat tccatatttt gcggcttacc cccctagggg
    19681 tggctctatg cctattttgc ctgagttgtg agggagagtt ccggccgggg cggcctgggt
    19741 gggagactgg ctcgggcggg cggtcaggtt tcgattctgg aaccggtgca ttagcgggtg
    19801 gttcaggggc gagcccaatg ggggggaatt cgtgcgcgcc ctcgttggtt cactttgcgt
    19861 ctggcccaga ctggatcgtg tacgaaggag atcttcttgc caactctgaa gctccgttac
    19921 tcggccccgc ccagcatgtg tgcctcaagc ctggcatgcc aatcaattgc cctcccgatg
    19981 ccacgtcgta cgacttcctg gggacggcct ggatcgcgga tctcggctca atcccggacg
    20041 cgcactggat ttgggcccca ggcgttcacg gcggggacat cagcgaattg gtaaagttca
    20101 cgttcgtgcg cgagttccag ctcggcgcgg ctccgagtgg ctacatcact ctcggcgtcg
    20161 ataactacgc ccgcgtgttg gtcaacggca tcttggtggg cgagctcgga agtattcaga
    20221 atccaatatt cgagtcattc aatcaactga ccacgttcga tctcaccccc gcactcaagg
    20281 ccggcatgaa cacggtcagc atcttcgccg cgaacggagt gaccgatatc gcaccgaact
    20341 gctcgtatac ttgcaaccca gctgggctgg tattcggcgg gaccctaacg tcggtaacca
    20401 cccgctgagg tccgctgcga cttagaacct agctaactac gggtgttacg gacacgaagc
    20461 gaatctcgac gaactgagta tcgatgtctc ggccgtcgat ttggacggac cggcagctcg
    20521 gatcgcaggt cgtcagctgc tcgcaatcgg agctgctcag gaacaactcc aagcgagttc
    20581 cacccgcaac tcggagcgtt ttccgatacg gttctatgat caaaggagtg tgagcgtcgt
    20641 cttctttggc gaccgcgttc aggtagtagt aggcgccggc taccttcagt ccccagctat
    20701 cgaaactgcc ggagtcgaac gcagcggttc gctcgagcca gaaatgatcg gcgtcgagtg
    20761 taccgtcagg gatggccgtc agctccacca gaccgctaat ctcgaccacg acgtcgtaac
    20821 tgacgctgct cgctcccgcg acctggagcg attgggaacc tggtaacggg cacgccgctt
    20881 ggcacaagta ttcagtcgat cctggtccgg cacaggtcga ttccagtcgc aagcttgcct
    20941 tgctcaagac gttcacgcat tgccccgcac tgcatgcttg aagggcttcg cacggtttcg
    21001 agcactcatg gttcgcaccc gaggagcctc ctgctccgcc gccaccctta ttggtcgtac
    21061 ctccagtgct tggcggtttg ccgcccgcgc tcgaggttcc gccctgcggg gttccaccga
    21121 ccgccgctcc gccggccgcg gtgtttccag cagtgctgat tccaccgctc gcgggcgtgc
    21181 ccgcttcccc tgcggcaccg ccgcccccgc ccagatccgc tcccgcgctg ccccccgttt
    21241 ccagcgtgcc gccgtcgccg ccggcaccac tctgatgatt ggggccgcca ccgcgagcac
    21301 cactgcctcc ctgacctgca gagttgatcg cgccaccttt gcccgcagtt cccccaacgt
    21361 cgccgggcac gctcgcgtcc acgattaagc tgcacgcgcc cagcactgcc atcgctgagt
    21421 gccccaatcc tgcagacatc cagattcggt tcttcactta ggtctccggt cgcttttcaa
    21481 cttggtccca cggtgatgcc ggtcgttcga cttggtcgcc ctcgaagatt gcgggttcag
    21541 aaggttcccc ggagctgaac cgcggaagga cctgccccca ccgagagcgt cggggtcttc
    21601 ttggtagttg cagcgtgttc gccgctcgaa ggggagctca gcgtcagcca aaggctgacc
    21661 ccgccggcga cggcggttgc gatcagaaaa ccgtcacaaa cggcggcgta agtcttggtc
    21721 gtgtcggact tggactgcat cgcagcgtga tcggagttgg gatcatcccg cagcttggcg
    21781 agtgagccat tggcagagag tgccagtatg ccggtcacgg ccgccccggt caccagtacg
    21841 ccagtgccaa cccaaccgat ccacttggcc gtggtgcgcg ggtcgctcgg cgcgacgtcg
    21901 gtgctcgctt tcccctgcgc ggttgggctt gtttccaggc gagcaaccgc ctttggcggt
    21961 gcagggggcg cgagcgtaaa cgcgacacgc tccgccctgc ttttttcgaa cgcaacccgc
    22021 cgagtctggg gtggatattc gggatgcttg acctcgactt cgtgagttcc cgggttgatc
    22081 ggcaaagggg ccggcagagg ggtcacaccg gccggtgtgc tgtcgattag aacttgggcc
    22141 cccgcaaggt ccgtttccac aatcaaggtc gccactcgcg cttccaagtc gcggatttct
    22201 ttctgaacgt cttcgcgccg ctgagcgttg atctcggcgc ccccttcggc caggtactcg
    22261 cgataggcct ggagcgcccc ggccgcatcc tgcatcaccc cgcgcacctg agcgatgttg
    22321 aagagaacct tgtaggagtg attgagggcc cgcgtctgga tcagctccgc caacgcctcc
    22381 gaataggcgc ccttcgtgta gagctcgacg gcccggttgt agtgcttgcc cgcttccgct
    22441 tcttgatcgg gggaaacagg ggtcacggac gctgggtccg ccgcgaacga ggttcgcgtt
    22501 cccgcgctga tcgacgcaat catcaaagct gcggcacacg agtatccgat tcttgtcatg
    22561 ggcaacctac gcgaacagat atcgaaacag accactcagc gcgaactcga accgatggac
    22621 cgagtaggat tgagggtgcg aaagtcaaca tcgaatccgg tcttgcgcgg cgttgggctg
    22681 gggctcggcg tcgagcgctc cagcaccacc ggcacatcca gatctctctc gaaactcagg
    22741 gggatgctgc gcgactgata acctggcgcg agcacggtca gaacatgagt cttgtcatcg
    22801 cgcgtccgcg ccccctgata cggattgcca ccgagtggtt tgccatccag aagcaagctc
    22861 gccgtcgggg gtttcgctga gattcgaacc cggatttcgg gtgacgcggc agcttcgcgc
    22921 tgcggaaggc tcgtcgcgga tgcgggcgga gtggacacga cgctcgtcga atccgcgggc
    22981 atcgcgggca cggtgggctc gcgctgggcc actggcggga cggttcctac ggcgctcccg
    23041 gcaggattca ccgcatggat gtcgggcgct tcggggcgag gatcgcggtt agccatccac
    23101 agcacgactg ccgccacgct caagcccaaa gcgcccccgg ccaaccaaac cgactgtctc
    23161 ctcacgccgc gcgagacagg gatcgcagac ccatgggcgg gcatcggtgg cggcggcgta
    23221 gtttgaatga ctggcgtcgt cgtgggcgcg gtggtcgaca gcgaaccgat gctgctcgta
    23281 tcggaagcat gaccgagctc gggcaacctc acgggcgcct tctcagccac gaagatcgcg
    23341 gcctcagtgt cgtctcgcat ggcctcgcgg atcactcgat tgagctcgct acgctcttcc
    23401 gcgaacagcg cctcgagtcg cgcgcctact tcccgcgcgg acaccccgcg cagttcgggc
    23461 acttccagca aatctcgacg gaagctgtgc gcgtctggat agcgcctggc cggctcggtc
    23521 gcgagcgctc gcaccacgat acgctccagc tcttcggata tgctcggcgc cgcctcacgc
    23581 aagctcggaa tcgcgccatc gcccatgcgt cgcagaattt catacacgtt cgagtcaccg
    23641 tgcatgcgac gcgcggccgc ggattcccag agcatcactc cgaccgcgaa aagatccacg
    23701 cgatgatcca atgggccgcc gcgcacctgt tcgggcgcca tatacgggac cttgcccttg
    23761 acgatccccg cctgggtttt gctcgattcc caggtcttcg caataccgaa gtcgagcaac
    23821 ttcgcatgtc ccgtataggt cacgaaaaca ttctggggcg acacatcccg atgcacgatg
    23881 tgcaacggag tgccgccata gtccgtgaga ttgtgagcgt actccagccc ctcgagaacc
    23941 tcgctgatca cgaacagctc tagcccaagc cccgacgggt cacgagacgg ccagctgcgt
    24001 tcctggagcc ggctgagagc ctggccctcg agaaactcca tggcgatgaa gtgttggtca
    24061 ccctctacac ccacctcgta agattgaatg atgtgagggt ggttcagacg tgccgcgagc
    24121 cttccctctt cttggaacat ctgcaggaac tcgggctgct cgtgcgcggc gatgtccgag
    24181 cgcaagacct tgagcacgtg aagtttcttg aaacccgagg ggcctgcagc gagggccaga
    24241 tacacttccg ccatgccgcc cgagcccaaa cgagccacga gccggtactt ggatagcgtg
    24301 tggtactgcc cagacatgcg agtgatggaa gggacacccg ggtccgcagc taactcttcc
    24361 tgttcgtgtg catcaccgta tcggtcgtcc cgcggcaccg ctgctcgctc actgaagtcc
    24421 tgatcatcga tcgaggcatt cacaaaacca ggctgattgg ccgaccttag cacatacaaa
    24481 gctccttggg ccagccagga cgtgtcggag agtaccccga gcggctgcga cgaaggcacg
    24541 ctgccccggg gcgagctctt gccttcggca tccgcccgcc gcgcatcgcc aagcgagctg
    24601 cgcgcggact ccggaacctg gggcgagccc cagtccacct cgagacaagc ggtgttcagc
    24661 atgaaacgac tcgaacctat ggagtcagcg ggagctttgc agtttccatg cgagccacct
    24721 ccggcgcctc gcccgggaga gaaacgcgat ttggcgaaca tttcgtcgac cccaggcaac
    24781 ttgaggttgc cgcgcaattc aaaccggcaa cccgcggttg cgcggtaagc aagcgcgaca
    24841 aacacgggcc gcccggtgat cctgctcaat cgcgatgtgc gcactcgcgc gaatcgctgg
    24901 agcacgcgag tggcaggact agaacagctc ccgcactttt tctgcagcga ggggctgaat
    24961 ggtctctggg aaaactcgga actgtttgta gttgttgaag tagactgcgt acttgatttg
    25021 gcccgccccg gtgccgccgt gtgtgaccag ccaagagtga aagcgttcga tatagtccgg
    25081 gctgtctcca ccgccaccct cactgtcggg gtctccgttg ctggcccatt ctccgatcgc
    25141 aaagggcttg ccgtgcgcct cggcgaaacg acgccaagac tctgctccga gcggagcgcc
    25201 atcagcgtac tcgccgctga gcttcttatc gaaggcttgc gcgtcgttta cccacggcca
    25261 ctggttgtac gtatcaatgg ctatgatgtc gacgtatgcg tccccggggt aggcatttcg
    25321 cacgtccaaa tcgagagtgc cgctagtccc atcgttcggg caccagacga gtgaggcatt
    25381 cggcaagtgg ctcttgaaga tgccgtggaa tcggcgccac gcttgcttga agttctccgc
    25441 atcgctgccc gtcacccgcc actgactatc gaacaggttg aattcgtgag cgaagcggac
    25501 gttcaggagc gactggtccc gcgtcttcca agccttttcg atattcccga ccgtgcgctc
    25561 ccaacgtgcg tcgaactcgc cgttggcggc ttgagcccaa gtctcgttct cgaaaatgcc
    25621 gccaatcgca ttttccaaaa gcttctgcca ggagccgtat tcgctctcgg gttgaatcgt
    25681 atactgttgc tcttgtaccg cggcactcgc gtcattccag gtccccgcac attgaacctc
    25741 cccgctctgg agccaattgg cgaggcgacc atccacgacg ccctcgcagc agaagcacag
    25801 cttcacctca ccaccgtccg cgtcacctgc cgcgcccgag ttcatgccgc cgagttcggc
    25861 ggtaccacca gaagctgttg agccgccccc cgaagtcgcc cccgcgcggg atgaggaagc
    25921 tccgccagcg tgtgcgccgc cagattgcgt gcctccagag ccattctgag gctcgcctcg
    25981 accagcagct cccgcgttct cgcttcctgc agcgccaccg aattccgatg tagtaccggc
    26041 attccctcgg tcgccgcctg attcgccgcc gccggtactt gcggcccggt tcggaaccgt
    26101 agtgtcgacg atgaagctgc acccgactgc ccagattgcg attccagcaa acgtgctcag
    26161 ccgctggtgc gcacgagcac ccttcgtgtt ggacatttca tattcctgta tgcgggcacc
    26221 tagagcgctg gttggcgcta aacgatatct cggcagacct gacactggcg ctacgccggg
    26281 tagatgcagg ttccgtgcaa ggaaagagcc gtgactctgc gatcgcaaag acgacggtat
    26341 ttccgcccaa gagcgcagct gatgcaactg aaggttgcgg tgcaatcaat cgccgcaacc
    26401 tctggttgcc gataatagtg aggagtgaag tcgattcagc caaacagagt caccccaaac
    26461 catgcttccg catgagcttc tggatcatct gccttgacac atcggacatc gcagacgccg
    26521 ccttcatgtt cccgctcgtt cgcgccagca gcgcttcgat atatgcacgc tcgaacgcat
    26581 cgctcgcgtc ccgacgggcc tcccggagcg gcggcaaggg accggtagcg gggagcggcg
    26641 aggcggccgg caaagcgccg cccgcgtggg ccacgtcggc tgcaaacacg cgctcgggtg
    26701 tgatgaggaa gcgctgcacg gcgttccaca gttctcgcac attgcccggc caacggtggg
    26761 tcttgaacat gttccagacc ggcggtggaa tgtcggagac gcgcaggttc gattgagctc
    26821 gcgccaggaa gtggtcgacc aagagcggca ggtcttccat gcggtcccgc aaaggcggca
    26881 cgcgcacgtg cgccgtcgcg agccggaaat acagatcttc ccgaaaattg ccgcgctgga
    26941 cctcggccgc gaggttccga ttcgtcgcgg agatcacccg aacatcgacc gtcaccgtgc
    27001 gctgcccgcc cagcctccgc acctcgcgcc gttccaacac gcgcagcagc ttgggttgca
    27061 gctccttcgg caattccccg agctcatcga ggaataccgt acctccatct gcctgttcga
    27121 aaagccccgg gcgcgccacc gcgcccgtat aggcgccgcg ctcttgtcca aagagctcgc
    27181 tctcaatcaa ggtcggagcg acggcgctgc agtcgaacac gacgagtggc ccctcgaagc
    27241 gtttactggc cccgtgcacc gattccgcca cgagctcttt gccacagccc gtctccccct
    27301 cgatgagcag cgtgacatcg gtcggcgcga tgcgcgccaa gtccgcgaac agctcacgca
    27361 tgcgtggaga gcgccccagc aagtcgccaa agcgttcggc caccgtctgc tctcgcgaca
    27421 ccgtctcggt caacggctcc acaaggatgc gcgtgtttcc gagctgcagc tgaaccgacc
    27481 cactgagcgt cgcgtcgacc acgcgcatgt tgccgaccaa gatgccgttc gtggaaccct
    27541 cgtcgcgaat gcgcaccccg cccgtcaccg gttccaacgc gcagtggtga cgcgaaaccg
    27601 tgttgtcggc cagcaccaga tcgttgtcgg acgcactgcc gatgcgcagc gtgctgctca
    27661 cgatctcacg cgtggttccc gcgtccggcc cgtccgtaac cgaaaccttg atcttcgagt
    27721 agctgacgag gatggggagg gctttggcca gtgtttcgtc tgtcatttcc gacccgcgct
    27781 gcggctcaga ggggcttatc tcggatcggc gcgggcagct acctccaagc tcacgagccg
    27841 ccgccaaaac ccgcacgtat tgacagtttc gagatgcccc
//
LOCUS       DASTKU010000095         3384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1132501, whole genome shotgun sequence.
ACCESSION   DASTKU010000095 DASTKU010000000
VERSION     DASTKU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132501"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFQ61_03355"
     CDS             <1..270
                     /locus_tag="VFQ61_03355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HET9953512.1"
                     /translation="AVVSREDLIESAFSTVICAPVYTARRGLSTEVHIGTAEGLRHDS
                     AILCDALVSLEKKRLTQYVGCLGHDKLLQLATALRIALDVEADEE"
     gene            566..802
                     /locus_tag="VFQ61_03360"
                     /pseudo
     CDS             566..802
                     /locus_tag="VFQ61_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            842..1201
                     /locus_tag="VFQ61_03365"
     CDS             842..1201
                     /locus_tag="VFQ61_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET9953513.1"
                     /translation="MSTGSSWRRKASFEVRQMCHVLGVSASAYYEWQREQESLHARQD
                     AELSDLVRGLFADFRGRYGAPRIQRELAKKGHRVSRKRVARLMREIGIRAKSARKYKA
                     TTDSNHRLPVAPNLLER"
     gene            1208..1435
                     /locus_tag="VFQ61_03370"
     CDS             1208..1435
                     /locus_tag="VFQ61_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HET9953514.1"
                     /translation="MDGPNRAWASDITCVWTRQGWMYLTVIIDLFSRKVVGWSLGERM
                     KASLVCEALDAAVRLRRPAPGLVFHSPVRAA"
     gene            complement(1632..2129)
                     /locus_tag="VFQ61_03375"
     CDS             complement(1632..2129)
                     /locus_tag="VFQ61_03375"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HET9953515.1"
                     /translation="MDVGFGDVTTPEPLLTEVPVLLDHPAPRLRAYARESAIAEKLHA
                     MVEFGLGSSRMKDYYDVWFLSKFYSFDAAVLCLAVRSTFERRRTAVPSSEPIGLSAEF
                     AADPIKLTQWRAFLRRSRLAEDAPPFPELLAEVHRFLMPILQAANAAEVPSASWLGSG
                     TWVSQ"
     gene            complement(2323..3024)
                     /locus_tag="VFQ61_03380"
     CDS             complement(2323..3024)
                     /locus_tag="VFQ61_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HET9953516.1"
                     /translation="MTGQRSRYYRRLGATQDQDSPWIDKSAIITKCRQKGSAQPTTPI
                     DLVGREGVVRLRDFVHAGFPKARILRLCEAGELERVGRGLYALPEREVSDSEALIQVA
                     RRAPQAVFCLLTALRIHDLTDQNPSKVWLAVDRKARRPKIEWPPLEIVWWSKEILAFG
                     VIHPTIGGVALPITSPAKTVADAIKYRNKLGADVAVQALRNYRRRRFAMDELFVAARF
                     CRVEKTLRGYLEVMS"
BASE COUNT          664 a         1079 c         1054 g          587 t
ORIGIN      
        1 gccgtcgtga gccgcgagga tctcatcgag tcggcgttct cgaccgtgat ctgtgcgccc
       61 gtctacacag cgcgacgtgg actgtccacc gaggtgcaca tcggcactgc ggagggcttg
      121 cgccacgatt ccgccattct gtgcgacgct ttggtcagcc tcgaaaagaa gcggctcact
      181 cagtacgtgg gctgcctcgg tcacgacaag ctcctccaac tcgccacggc gttacgaatc
      241 gccctggatg ttgaggccga cgaggagtag cggtcctgga ggatttcgag gagctcgagt
      301 gccccgtcgt cctggtctct caccgcgcgt ggctgatcga ggtgttatgc aggctcacac
      361 tcccccgtgc ttctcgagcg cgccgctgat cgccatcctg atcttccatg gaccagttgc
      421 gatcagcgcg cttacgaagc cctttgaact cgaatattcc cccctggcaa gttgccgcgg
      481 cagcctatag tggagctgat ccagtttccc ggacaccaga tgagccagta gctcctggag
      541 tccggaacat ggcaagcagc gaaagaagta caccgaggag ctcgagcgaa acgcggtgcg
      601 catgatgcgc aatcgcggcg agcgcaccgt aggccagatt gctgacgccc tgggcgtggg
      661 agccaaccag ctgcaccgct gggcggctca atacgagcag gaagcggtcg ccaagcggaa
      721 cgagaagggc gagacgctcg aagaggagaa ccggcggctg cgcaaggaga acgagcggct
      781 ccggatcgcg aaagccatct aaaaaagagc cgcgtccttc ttcgccaagg aggacgagta
      841 gatgtctacc ggttcatcct ggcggaggaa ggcaagcttc gaagtgcgcc agatgtgcca
      901 cgtgctgggc gtgtcggcaa gtgcctacta cgagtggcag cgagagcagg agtcgctcca
      961 cgcgcgccaa gacgccgagc tcagcgacct cgtgcgcggc ttgttcgctg acttccgcgg
     1021 ccgctatggg gcgcctcgca ttcagcgaga gctcgccaag aagggccacc gcgtgagccg
     1081 caaacgtgtg gcccggctga tgcgcgagat cgggattcgg gcgaagagcg cccgaaaata
     1141 caaggcgacg acagactcca atcaccgact accggttgcg cccaacctgc tcgagcggta
     1201 gttcatcgtg gacggcccaa acagggcgtg ggcgagcgac atcacgtgcg tgtggacccg
     1261 gcaaggctgg atgtacctga cggtcatcat cgacctgttc tcacgcaagg tggtcggctg
     1321 gagcctgggc gagcgcatga aggcctcgct cgtctgcgaa gccctggacg ccgccgtgcg
     1381 cctgcgacgc cccgccccgg ggctcgtctt ccacagccct gttcgggccg cgtagcggct
     1441 gccaggatcg acaagaacga gtacgcccgc acgctcccgc tcacggacgc atacctgttc
     1501 agccaggtgc gctggtccca cacgcctatt gagaccattg ttgaactaac cgcgacctac
     1561 gtcgtcggct ggagaagtat tcgtcaacga cgctgccagc caggcgtgta cgcgagatgc
     1621 tcgtccgcga gctactgcga cacccaagtg ccagagccca gccagctcgc cgagggcact
     1681 tccgcggcat ttgcggcctg cagaattggc atcaggaacc tgtgaacttc ggcgaggagt
     1741 tcaggaaagg gcggcgcgtc ttccgccagt cgcgatcggc ggagaaacgc cctccattgc
     1801 gtcaacttga tcgggtccgc agcaaactcc gcagagagac caatcggttc gctacttggc
     1861 actgcggtgc gtcgccgttc gaaggtagag cgcaccgcca ggcaaagaac cgccgcgtcg
     1921 aaggagtaaa acttcgaaag aaaccacacg tcgtagtagt ccttcatccg gctgctcccg
     1981 agtccgaact cgaccatcgc gtgcagcttc tcagcgatcg cagattcacg tgcatacgcc
     2041 cgaagacgag gcgctggatg gtcgagcaac actgggactt cggtcagcag cggctcgggc
     2101 gtcgtcacat ccccgaagcc gacatccact tggacgggcc ttcagcgcac taccgagctg
     2161 cgccgtgaac cgcaatcgaa tgccgtcgta cacggcctcc tcgcgaatgg cttccgcggt
     2221 cacggtgtca gcaaggaaaa ccaagccatc ctccggcttt ttggagcagg cgcgccttga
     2281 tcgaggctgc tacgttcctc ggaccctccc ccttcgggcg cgtcacgaca tgacctcgag
     2341 atagcctcgg agcgtttttt ccacgcgaca gaaccgtgcc gcaacgaaca gctcatccat
     2401 cgcaaagcgc cgccgtcgat agttacgcag cgcctgcacc gccacgtccg cccctaactt
     2461 gttccggtac ttgatggcat ccgcgacggt cttcgcaggt gaagtgatcg gaagcgcgac
     2521 gcctccgatc gtgggatgga tgacaccgaa tgccagaatt tcttttgacc accagacaat
     2581 ctccagtggc ggccattcga tcttcgggcg acgcgccttg cggtccaccg caagccagac
     2641 cttcgagggg ttctggtcgg tgagatcgtg aatgcgcagg gcggtgagca ggcagaagac
     2701 tgcctgcggt gcccgcctgg ccacctgaat cagcgcctcc gagtcactga cctcacgctc
     2761 cggcagggcg taaagaccgc ggccaacccg ttccagttcg cctgcctcgc agaggcgaag
     2821 gatccgcgcc ttggggaaac ctgcgtgcac gaaatcacgc agccgaacta ccccctctcg
     2881 accgacgagg tcgatcggcg tggttggctg ggcggatcct ttttgtcggc actttgtaat
     2941 aatagccgac ttatcgatcc aaggcgagtc ttggtcctgg gtggccccga gcctgcggta
     3001 gtacctacta cgctgccccg tcataccctc gaggagcacc ttgcgatcga ggggtacgct
     3061 cactcggaac gaggtgacca cctaccctga cgtctcggcc gtatgcggtc cgtatgaaac
     3121 ggacccgcag gacaagaaca caatcgtaaa cccgcgcgtg ctggggcgag aagctcgaga
     3181 gttatcagcg catcccctct gtccaggccg tcgtcctggt cagtcaccgc gagcggctga
     3241 tcgagatgtt cgatcggagc agcagttcgc ctgtggcagc gcattcgggg caacgctcgg
     3301 ggtcgacgag atcttcgcgg tggtagccgg tgtgaaccgg gcgcccctcg ggtgcggggc
     3361 gaaccggagc tgcgcgacag cgtc
//
LOCUS       DASTKU010000096        21590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_687321, whole genome shotgun sequence.
ACCESSION   DASTKU010000096 DASTKU010000000
VERSION     DASTKU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 21590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21590
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687321"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2318)
                     /gene="ligD"
                     /locus_tag="VFQ61_03385"
     CDS             complement(<1..2318)
                     /gene="ligD"
                     /locus_tag="VFQ61_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET9953517.1"
                     /translation="MPRTRRPSGVSADTSTAAPATSALAPSTPGSATPLQEYRRKRDP
                     LATNEPFGAERKASALETRSGRFVVHLHSARQRHYDLRLQIGRSLASFAVPRGPSLDP
                     AQRRLAVLTEDHPLEYVDFEAVIPAGNYGAGPMICWDVGRVSYLEAAAETGVQNGKLD
                     FWLQGFKLRGRFALVRTKRGEGNEWLLIKKQDAHSRETGDILEEQPESVLSGLTVDQL
                     EQRRAIASEIERQAVAYGAKRADIDAAHLQPMLCRDSEASHAEGDAATELAHPALIDA
                     ERVFELKVDGVRIVATLADGEVSLRYRNGRIATSSYPEIVRAVQTLPVDRAVLDGEIV
                     TFDERGRPSFQRLGPRIHAQRPEDVSRVAAEIPVVYWVFDLISVNGLDLRPLALRERK
                     TLLTALVRGRGLVRRLDHLEGQGAHLFRFCSEQGLEGVVAKRRDSRYRPGPIRSDDWL
                     KIKCLIEDDFVVIGLVRGKGAREQLGALALGSYVDGKLIYRGRVGSGFDDRSVKEMLA
                     LLTPLLGAPCLLEPPLPVETKPVLWVEPRCVVRVRFSGFSEDGVLRHSVYVGLRPDVS
                     PEDCHAAPAAHEEDDPTGAVGVSAVGVSGATAPGTSTLSANAPANSRSAATKVQLTNL
                     EKVYFPEQGYKKGDLIDYYAAVAPVMLPFLKDRPVMLVRYPDGIHGKNFFQWHVPRGT
                     PSWIRTLTIRDDEDASREKTVFLLDDLDALLHVANLGCIPLHVLACRAGSLEFADYLT
                     FDFDLGEQPFLRAVELSLALGELLEEIGLRGF"
     gene            2488..3354
                     /locus_tag="VFQ61_03390"
     CDS             2488..3354
                     /locus_tag="VFQ61_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET9953518.1"
                     /translation="MAPRATASCTISFGLVSIPVKLYTAAASEQVGFNMLHKKCGSRV
                     KMQFICPTDNEVVERNESIKGFEYARGQYVLFTEEELKALEGARDNSIEITEFVPLAT
                     VDLVQVEKSYYLGADKGGDKAYRLLSQSMAGKDRVAVGRWANRGKEQLVLVRPYGDSG
                     LLLHQLYYANEVRSFEEIDTGAKLSFSDKERELADRLIDQLTVEEFEPEKYHDSYSER
                     VMQAVEQKAQGQEITVSQEAPKAQIIDLFEALKKSLETAAAPAAANAGELKPPKKAEA
                     ERKPGKKAGKAG"
     gene            3660..4373
                     /locus_tag="VFQ61_03395"
     CDS             3660..4373
                     /locus_tag="VFQ61_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953519.1"
                     /translation="MSQSMRSTRTARLPFARSCLALALVGALIVPPRSAAACGGEWYP
                     ALMDQEPDLRPKVIPLAEKALEQGRVAAAAGIVIRVIPHIKTLKPQSSSLVERAARVL
                     AVAVTRSGGKLPVESEVPSEIQDTWLGKTAKDRAANLQFAARTLSAISDLKKDDPAVQ
                     TELAEALAQLPESRARALALLEDLARRDLVASAEGYAALAQLRARAGDASGGQLALAR
                     CQSMSKAKAVCSSTLTPAS"
     gene            4422..5825
                     /locus_tag="VFQ61_03400"
     CDS             4422..5825
                     /locus_tag="VFQ61_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase Cbb3"
                     /protein_id="HET9953520.1"
                     /translation="MRLSTTNAVSFGQPLAARANPLLRAAILSALLSASGCGKHQTES
                     SAAPSTPRATDTRIAEGGVASRNPSSAPTSDAEHGGAEHGRALVERYQCNRCHEGTAQ
                     AAVPREQHCVNCHRDILADRFPAAADKLSRWKGHVNTVRSVPSLIGVGTRLKASFIAE
                     FLLSPYDLRPELEPTMPRLALSPSEARDIAAYFVSLGPKPTPPSPAPLVEQAGSGTPS
                     DPSPARRDSDGDAQNGRALLSTRGCGNCHHLSRAPLPVTPAPGAAQRSDAVLLAPDLG
                     HVRERWESVELVAWLRDPSSLKPDTLMPTTGFSQAEARDVATYLLRADLPEAPAGAKF
                     LRLPTLDRPVTYAEVDEKVLRITCRHCHANPDIALGDGGPGNTGGFGFRARGIDLSSY
                     GGVLSGYLDEAGQRRSLFEKSASGEPRLLAALLARHDEEAGHAQSDVRGMPLGLPAVS
                     AAQIQLVESWIAQGRPR"
     gene            complement(5901..6644)
                     /locus_tag="VFQ61_03405"
     CDS             complement(5901..6644)
                     /locus_tag="VFQ61_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953521.1"
                     /translation="MPQTVSPQEGAASPAQSPEPAPPPEPLPPPLVPPRVGRIVLEPP
                     PPPVPAPDPSVRRHDGFYLRLGLGAGTIAARIDSPAPYGKLNASGGGVGLEIALGGTV
                     AEGLVIGGGLYVQAASQVRWKGDGLRSFTEGRTSLTGQEGGVSVLGVLVDYYPNPRDG
                     FHVQGALGIGSLNFGRDSNRVPSEDWTGGGGGLMLGAGYEFWVAPQWSLGGLGRLLMV
                     GGTLRGEDSSAEFDARGVALSLIVAATHH"
     gene            7156..9612
                     /locus_tag="VFQ61_03410"
     CDS             7156..9612
                     /locus_tag="VFQ61_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET9953522.1"
                     /translation="MNSLPSTDERSELEQHARVALDWPELLAAIAEQAHGERAREALM
                     TLRPAATRAEALLRTARLRSLLELQRAGVDLPVAAVPELTEALARIRLGASAAGAELV
                     GVLRVLRLARELRERLVEHADRAPLLSEWLSTDPKLDRTMARLELCLEPSGNVADAAS
                     EALVEARARTRAAREELKRRSSDLVQRYADVLSGSYSTERDGRHVLPVRADAHYRVEG
                     IVLGVSASGGTLFVEPRELTELGNRLRVCEAGVAREEARVLAQLSDLVREQQPEIECA
                     IEACVNADILSAIARFAHQTQALPLDVAERPVIDLRAARHPLLSLSKRPVVANDVRLE
                     AGQGLVISGPNAGGKTVTLKLLGLFAWMIRAGLPLPTGEHSEFGWFDCVLSEIGDEQS
                     LLHSLSTFSAHVQHLSQILQHTGPGTLVLLDEVAAGTDPEEGAVLACAVLEALAERGG
                     AVVTTTHYERLKELAAKPGRLRNACVGFDFAQMSPTFRLTLGMPGPSSALLVAARYGI
                     PAELLQRARALLPEAAIDREATLQELAKQRERVDQDRTRLESDRREHEALARELDQER
                     REQRAAAERSIEAEARKLLRDVQTARSELYQAREKLKQERLDAAQLKALENSVNRVAS
                     QVAIGGPFAVPSRAASSQSASGQSAGGQAATLALVTPERLRPGTQVRLRENGALATVL
                     EAPQAGEVRLRVGALRMTRPLGALSPVAHHGVQANSAPKRVRRAELPKAQIAPQRTRE
                     NTLDLRGIRVDDVASQIDAFLDRMLGEGEPVGFVLHGHGTGAVRQATREHLASSSYVE
                     HSRAAEPDEGGNAFTVFWVR"
     gene            9800..11941
                     /locus_tag="VFQ61_03415"
     CDS             9800..11941
                     /locus_tag="VFQ61_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HET9953523.1"
                     /translation="MPDTASRPTASSERPRPGADPSPSSGSEGPGPSADSVASTNAAS
                     ASSAGAPSSPNPSNGSPPEPRSSPRASSPGALRSLLPTAVPQPQAHAAPNSHAHGAHG
                     NMTKVTLAALGIVFGDIGTSPLYSMRECFHLISPTRANVMGVLSLVFWSLVVVVSLKY
                     VVYVLRADNKGEGGILALMALSSSTLKSSKWHLVLVSLGVFGAALLYGDGVITPAITV
                     LGAVEGLSVAAPKLEFLVIPIAIAVLIGLFTIQRRGTAGIGALFGPVMLVWFGTLAVL
                     GVINLHKDPAVISALLPHYAWDFLWRKGHIGFSVVGAVFLVVTGGEALYADLGHFGRA
                     PIRRAWYGMVFPSLLLNYLGQGALLIHHPEAKADPFFELAPRWALYPLVALSTAAAVI
                     ASQALISGVYSLSHQGTMLGLLPRTTIRHTSAEERGQIYVPTVNWLLMLATIALVLVF
                     RSSSNLAAAYGIAVTLTMVITTVLAFILVRKQWKWNLWLASGVTIGFLIPELTFTAAN
                     FTKFWHGGFFPLLVGALLFVMMTTWKRGREVLAQRFREQMLPLEDFFDLIRVEIPARV
                     PGTAVFMTSSRDGTPPALLHNFLHNRVVHQHVVLLTITTEDAARVSERERYTTEELPQ
                     GFLRIVAHYGFMEQPDVPRLLGRAGVVGASLEGTTFFLGRETMIATARPGMARWRIHV
                     FSFLSRNSQPATRFFCIPPDRVMEIGAQIEL"
     gene            12019..12639
                     /locus_tag="VFQ61_03420"
     CDS             12019..12639
                     /locus_tag="VFQ61_03420"
                     /inference="COORDINATES: protein motif:HMM:NF021512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HET9953524.1"
                     /translation="MVNESRSNREQSASDEQRSNPDQGYVAFARSRRVAHGSLREVLP
                     VLKRRADADASDLVLVFELETGRQVDFDLRGTLEEVLDREAPERPRGPGRPKLGVISR
                     EVSLFPRHWEWLEQQPNGISAALRRLIEEAIKAAPGKDRARRIRAALNPFMTALAGDR
                     EHYEEATRALYAGETERLETLIQRWPKDIREHVLERARAADLAEAD"
     gene            complement(12731..14494)
                     /locus_tag="VFQ61_03425"
     CDS             complement(12731..14494)
                     /locus_tag="VFQ61_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953525.1"
                     /translation="MHSNKLGLGEGISLATPKRSPWVAGFAVGLLCGCSGNVAKSIDA
                     GGTGGSSAGSTHGMMGGDTAWEAGRGGAGQADGGFQSKGGSESKGGSKSEGGAQGGEI
                     GGVSQAGAAGEVQNGGSGWGGSSAGPQPLPYVKSGKRLKATGFVADGAYSFTGFEDTE
                     WGPCQFASRGDRVVCLPARSARLLFLDAQCTQPATWFPGDAEVGDPVTAEGPSDSNVC
                     TGDLPEANSAYVLGERVYEEALPPSQATHDIFKMSAKGCVPAEHMGKIDPPVHRLEPI
                     PDSELVFGQLRSIDLHEHLGLTRVQMEDGAELTWSLTTTGHRSCVLAESADCVPAPLA
                     APNTPFANADCSEYTWNSHPSACGTADYMVLDGRVHELVPAAAQFDQEWTGKSSVPEC
                     KPLSTPSEYAMQPGKDVTGTFASMQGLEVVPLGNGELRSRMFVWREPSSGALWHLAPR
                     QPRAAIWLPDGRANFWLPDGRACRVEPAVDGTLRCAAIGNLYETGFADPECTRRVYED
                     FSQSSNPAAELPEPFGVGEYASDGRLVAAYTVRAHSGPIYLSQGESICVEASVSELIR
                     FWERDQRTDAAEMPIVTRAEL"
     gene            complement(14622..15929)
                     /locus_tag="VFQ61_03430"
     CDS             complement(14622..15929)
                     /locus_tag="VFQ61_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953526.1"
                     /translation="MLCGLLVPAACAEDDAPGTAAAIAGNGGASQTTPTGGATSASSA
                     GANSGGAGAGASSADASSADAGAAGTSQPGLEVVQVTTLTTVQASAGLALAPEPELQL
                     PEFVERERAAQEQSSTGGLEDRAEIDTALVFRAAAGDVRGGALSVSPIFSAQRSWPVE
                     WGFPALRADRGGLVWGLALSEPDDSTVSSDWLRTTEWTGWKAWGGLAVRAVDDDSNQA
                     PGNPAPSAPAAILSFAARGVFSPLRLSSDARDLIISNPTNQTIAQALLIYSHSEGVGV
                     REVLDLGPGERRETTLGPKEQPIAALLERARDTLKDFFVERLGEELGATIAESKSIPF
                     LETPGLRLIYMLDAAASPIAMNFSSPVDRAAPVIVAHSEVLTPAEESRVLSLLARDAA
                     PTLAETTSELGRFTQAKLEFAAQQDEPLVRERAEQLLHELLQP"
     gene            16105..17010
                     /locus_tag="VFQ61_03435"
     CDS             16105..17010
                     /locus_tag="VFQ61_03435"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9953527.1"
                     /translation="MNLERLELFLRIVDTGSVSAASREAHLTQPAVSRSLKLLEEDIG
                     SDLFDRQGRGLLLTAAGRALVPCARQLVREAERARLQVQRAAQSACFDLRLGAVDSIG
                     THVLPNCLMAVQERHPEIKIKLWMGRTQSLLERVERGTLDLALVAYSGPPSERAQRIG
                     SYGLKYYGHRQRYPELATLSSELELRRLPIVEIEALPGQPTLIQDDSDAFAVTQNLSA
                     VKALVLAGLGVGQLLPFMLTPEERDRLVCAGSIRHDPDCGIYGVRSGRALTTVETAIQ
                     DDLLATLKAQLAPLPTDEGSLGGAY"
     gene            complement(17004..17939)
                     /locus_tag="VFQ61_03440"
     CDS             complement(17004..17939)
                     /locus_tag="VFQ61_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953528.1"
                     /translation="MKELSIPLGCLVIVACRQPSSSESPSGRDAVALEQPAPATPAEA
                     TAGLHGQGTQAPSVRDAKTMPSAMTSEDQATLAPLRDVSWLEPLPLEGGELAYVTPAL
                     GAREPRPLMVGVHGAGDRPEWACGGWRLAASEYAFVVCPRGLPMGSQRFAWDAPRTIA
                     ERVRLAIQAVKQRYGTHVAEGPTLYVGFSQGATLAEAALLAEPGRFPNVALAEGGYNL
                     LRDAGFLDRLHGQGTSRLLGICGSNACFATLRSAASHVSRAGIEFQVAGDPLSGHNLN
                     QRMQDALQQAWPNFVRGLPNWAGFPAYLERRRRSQ"
     gene            complement(17936..18943)
                     /locus_tag="VFQ61_03445"
     CDS             complement(17936..18943)
                     /locus_tag="VFQ61_03445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET9953529.1"
                     /translation="MVLPLSALDLVPLGSGYSSHAAVHASVALARALDNWGYQRVWYA
                     EHHNMPGIATTTPALLIAHVAPRTQRIRLGAGGVMLPNHAPLQVAEAYRLLEALHPGR
                     IDLGIGRAPGTDPVTASALRRGRYGGSDEFPGLLAELRGFGTQSLPAGHPFSQIVAMP
                     DDVTLPPIWLLGSSDYSARLAAQLGLGFAFAGHFSPEPPEWPLQLYRESFQPGPFREP
                     HAIVALSVFCGETEVAAKRLASSMLVAFAQLRAGRPSRLMSPEQAEQYRFSPEEEAAI
                     GFARRLQIVGTPAYVRERIEAVAQSTAADEVMVATHAYVPEQRLRSYELLAREFELGR
                     G"
     gene            19615..20658
                     /locus_tag="VFQ61_03450"
     CDS             19615..20658
                     /locus_tag="VFQ61_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9953530.1"
                     /translation="MRHRIYGQAIDSVGRAATRALLVCASAATLACIPPLEEPKTAQG
                     EAAQAPPSDELTRKGLDAIKAKDYAKAKQFLAEARKANPKDAQNPYYLGVAEQESGNR
                     AGAIAAYRECLALDPKFTEASINLGAILLEGHNPAEVAESVKVSDEGLKHNPKSADLA
                     KNKAVALADSGDFAAAAEAYKKLMEAAPQDLRLRYDYARILSDSGKIPEAIAALKPVG
                     ESTDVKLLNAAGSLNAQLGAYEECVTVFDKVIQAGPNVSALVNRGNCKRGLKDLKAER
                     ADYEQALQIDPNYAPAHLSLGLNLFYVGKDRAQAIAELQKAKQLGQGTPVAEEADKML
                     AELDRASGKKKKK"
     gene            20914..21381
                     /locus_tag="VFQ61_03455"
     CDS             20914..21381
                     /locus_tag="VFQ61_03455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET9953531.1"
                     /translation="MPNPELELRSFSHQDPADREFVSQLSREAFSEFSLNAPSTTLHM
                     ATHEASWIALESGTRVGLAVCVIQPSELAELCAIAVIARERGRGIGRALLSHAERATR
                     ARGAPGMLLHTATANVAALDLFLRNGYRIRRRLPRYYRNVYEACELVKHWRRI"
     gene            complement(21378..>21590)
                     /locus_tag="VFQ61_03460"
     CDS             complement(21378..>21590)
                     /locus_tag="VFQ61_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953532.1"
                     /translation="VTPHAVGFQVSAATLGSSLVPSLTGVLVGHLGLSAIGLLIVVAS
                     AALLVVHESMLRMRPPRPTPAIHLSV"
BASE COUNT         4011 a         7164 c         6713 g         3702 t
ORIGIN      
        1 caaaacccgc gtaggccgat ttcttccagc agttcgccga gagcgagcga aagctcgacc
       61 gctcgcagga acggctgctc acccaaatcg aaatcgaacg tcaggtagtc cgcgaactcg
      121 aggctccccg cgcgacacgc gagcacgtgc aacggaatgc agcccaagtt tgcgacatgt
      181 aacagggcat ccaaatcatc caacagaaag acggtcttct cgcggctcgc gtcctcgtca
      241 tctcgaatgg tgagggtgcg aatccagctg ggagtgccgc gcgggacgtg ccattgaaag
      301 aaattctttc cgtggattcc gtcgggatag cgcaccaaca tcacggggcg atctttgaga
      361 aacggcagca tcaccggagc caccgcggcg taatagtcga tgaggtcgcc cttcttgtaa
      421 ccctgctcgg ggaaatagac cttctccaaa ttggtgagct gcaccttggt tgccgcgctg
      481 cgcgagttcg ccggtgcgtt cgctgacagc gtcgaagtgc cgggcgcagt ggcccctgat
      541 acaccgaccg ctgatacacc gaccgccccc gtgggatcgt cttcctcgtg agcggccggc
      601 gcggcgtggc aatcttcggg agacacgtcg gggcgcaatc caacgtacac ggagtgtcgc
      661 aagacaccgt cttcgctgaa gccgctgaac cgcacccgaa ccacgcagcg tggctcgacc
      721 cacaacacgg gtttggtctc taccggcaag ggtggttcga gcagacacgg cgcgcccagc
      781 aggggcgtca agagcgcgag catctccttc acgctccgat cgtcgaatcc actcccgact
      841 cgtccgcgat agataagctt gccatcgacg tagctgccga gcgcgagcgc gccgagctgt
      901 tcgcgcgccc ctttgccgcg caccaagcca atcacgacga aatcgtcctc gatcagacac
      961 ttgatcttca accaatcgtc gctgcggatc ggcccgggcc ggtaacggga atcacgccgc
     1021 ttggcgacga ccccttccaa accttgctcg gagcagaacc gaaacaggtg cgcgccctgg
     1081 ccttccaaat ggtcgagacg gcgcaccaga ccacgcccac gcaccagcgc ggtgagcagc
     1141 gtcttacgct cacgtaaggc caacggccgc aaatccagac cattcacgga aatcagatcg
     1201 aagacccagt aaaccaccgg gatttcagcc gcgacgcgag acacgtcctc cgggcgctgg
     1261 gcgtggatgc gaggcccgag ccgctggaag ctagggcggc cgcgctcgtc gaaggtcacg
     1321 atctcaccgt cgagcacggc gcggtcgacg ggcagcgtct gcacggcccg tacgatctcc
     1381 ggatagctcg aggtggcaat gcgaccgttg cggtaacgca agctcacctc tccgtcggcc
     1441 agcgtggcca cgatccgaac cccgtccacc ttgagctcga acacgcgctc cgcatcgatc
     1501 aacgcgggat gagcgagctc cgtcgcggcg tcaccctcgg cgtgggacgc ttccgagtcc
     1561 cgacacaaca tgggctgcag gtgggcggcg tcgatgtcgg cgcgcttcgc cccgtaggcg
     1621 acggcctggc gctcgatctc ggaagcaatg gcgcgccgct gctcgagttg gtcgacggtc
     1681 aatcccgaca gcacgctctc gggctgttct tccaggatgt cacccgtttc tctcgaatgg
     1741 gcgtcctgct tcttgatcag gagccattcg ttgccttccc cgcgcttggt tcggaccagc
     1801 gcgaagcgac cacgcaactt gaaaccctgc agccaaaaat ccaacttgcc gttctgaacg
     1861 ccggtctcgg ccgcggcctc gaggtagctc acccgcccga cgtcccagca aatcatcggg
     1921 cctgcgccgt agttaccggc cgggatgacg gcttcgaagt ctacgtactc aagagggtga
     1981 tcttcggtca gcaccgccaa gcggcgctgt gccggatcga gactcgggcc tcgcggaacg
     2041 gcgaagctcg cgagcgagcg tccaatctgt aaccgcaaat cgtagtgacg ttgccgcgcg
     2101 ctgtgcaggt gaacgacgaa gcgtccgctg cgcgtctcca gcgcactggc tttgcgttca
     2161 gctccgaaag gctcgttggt ggccagcgga tcgcgttttc ggcgatattc ctgcagcggc
     2221 gtcgccgagc cgggcgtcga tggagcaagc gcagaggtcg ccggcgctgc cgtggaagtg
     2281 tcagctgaaa ctccggacgg gcgtctcgtg cggggcatcg cgcaaacggc aggatagccg
     2341 ctttcgcgaa aagacgggaa cgccccgacc tgatcgcgcg cgaaaatcgt gggctgcctc
     2401 ggcccggagc cactactggg caccgggtaa ccagaaactc gccgataagt tttacgtcac
     2461 gccgaccgat cccgtaccat tcgtttcgtg gcaccgcgcg caaccgcctc ttgcaccatt
     2521 tcgttcggct tggtttccat ccccgtaaag ttgtacaccg ctgcggcttc ggagcaggtg
     2581 ggcttcaata tgctgcacaa gaagtgtggc tcgcgggtga agatgcagtt catctgcccg
     2641 acagataatg aagtcgtcga gcgcaatgag tcgatcaaag gcttcgagta cgcgcgcggg
     2701 cagtacgtac tgttcacgga agaagagctg aaagcgctcg agggcgctcg agacaacagc
     2761 atcgagatta ccgagttcgt gccgctggcc acggtcgatc tcgtccaagt cgagaagtcc
     2821 tattacctcg gcgccgacaa aggtggagac aaagcctacc gcctcctcag ccaatccatg
     2881 gccggcaaag atcgggtcgc ggtggggcgc tgggccaatc gtggcaagga gcagctcgtg
     2941 ctcgtccgcc cgtacggaga cagcgggcta ttgcttcatc agctgtatta cgcgaacgag
     3001 gtccgctcgt tcgaagagat cgataccggc gcgaagctct cgttcagcga caaggaacgc
     3061 gagctggccg atcgattgat cgaccagctc acggtcgagg agttcgagcc ggagaagtat
     3121 cacgactcgt actccgaacg ggtgatgcaa gcggtcgagc aaaaggccca aggtcaagag
     3181 atcaccgtct cccaagaggc cccgaaggcg caaatcatcg atttgttcga ggcgctcaag
     3241 aagagcctcg agacggcggc ggcacccgct gcagccaacg cgggcgagct caagccgccc
     3301 aagaaggcgg aagccgagcg caagcccggc aagaaggccg gcaaagccgg gtaaagatac
     3361 gttgcgggag cgccgcctct tccctccgga agaggcggtt cctcgataag gctagtcgca
     3421 aggacgcgcg cttgcgcgtg cggtcgtggc ccgcgtggtc ggctgcggct cgacgcacac
     3481 gcgaacgtgt atgagcaaac tcgcgagcat cgcgaaacgg gcgtggatgc gcggttttca
     3541 gccgtcgttc gcctggcacg ccggttgctg aagagggtta tcgagctgac gcaaagcgcg
     3601 gcgaagttcc gccgcgtcga gggcgtcgct ccttcaactc caagccccgg aggtttgcca
     3661 tgtcgcaatc gatgcgttcg actcgtactg cccgcttgcc tttcgcccgt tcctgcctag
     3721 ctctcgcgtt ggtcggagcg ctgatcgttc cgccccgctc ggccgctgcg tgtggcggcg
     3781 agtggtaccc ggcgctcatg gatcaagagc ccgacctgcg tccgaaggtg attcccttgg
     3841 cggaaaaggc attggaacaa gggcgagtgg ccgcggcggc gggaatcgtg atccgagtca
     3901 tccctcacat caagacgctc aagccgcaaa gctcgagcct ggtggagcgt gctgctcgcg
     3961 tccttgccgt cgcggtgact cgaagcggcg gcaagctgcc ggtcgagagc gaggtaccga
     4021 gtgaaattca ggacacctgg ctcggcaaaa ctgccaaaga ccgcgctgct aacttgcagt
     4081 ttgccgcgcg taccctgagc gcgataagcg atctcaagaa ggacgatcct gccgtgcaga
     4141 cggagctcgc agaagccttg gcgcaactgc cggagtcacg tgcacgcgca ctcgcgctgc
     4201 tggaggacct ggcgcggcgc gatctggtcg cttctgcaga gggctacgcc gcgctcgccc
     4261 agctgcgcgc gcgcgccggt gatgcgagcg gtgggcagct agcgctcgcg cgttgccagt
     4321 cgatgtccaa ggccaaggcc gtttgcagct cgacgctgac cccagcctct tgattcgtgc
     4381 acttgtccgc ggcttggagc cttcgaagac cggtgttccc tatgcgtctg tcgaccacga
     4441 atgcagtctc tttcggccaa cccctcgccg ctcgcgcgaa tccgctgctg cgggcggcaa
     4501 ttctgagcgc cctgctgtcc gcgagtggct gcggcaagca tcagaccgag tcttctgccg
     4561 ccccgtcgac tccgcgcgcg acggacacca ggattgccga aggcggcgtc gcttcacgaa
     4621 acccgtcgag cgcgcccacg agcgacgctg aacacggcgg cgctgaacac ggccgcgctc
     4681 tagtcgagcg gtatcaatgc aatcgttgcc atgagggcac cgcgcaggcc gcggtgccac
     4741 gcgagcaaca ctgcgtgaat tgtcaccggg atatcttggc tgatcggttt ccggccgccg
     4801 cggacaagct cagtcgttgg aagggtcacg tgaacacggt acgcagtgta ccttctttga
     4861 ttggcgtcgg aactaggctg aaagccagct tcatcgctga attcctgctc tcgccctacg
     4921 atctccgccc cgaactcgag ccaaccatgc cgcgcctcgc gctgagtccg agcgaggcgc
     4981 gtgacatcgc cgcatacttc gtgagccttg gcccgaagcc cacaccgccg tcgcctgcgc
     5041 ccctcgttga gcaagcgggg agcggaacgc cgagcgaccc atcgccggca cgccgcgatt
     5101 ccgacggcga cgcccagaat ggtcgcgcgc tgctctccac tcgaggctgc ggcaattgcc
     5161 atcatttgtc ccgagcgccg ctgccggtga cgcccgcccc aggcgcggcg cagcggagcg
     5221 acgcggtgtt gcttgcgccg gacttgggcc acgtacgcga gcgctgggag agcgtcgagc
     5281 tagtggcttg gttgcgcgat ccgagcagcc tgaagccgga caccttgatg cccacgacgg
     5341 gcttctcgca agcagaggcc cgagatgtcg cgacgtacct actgcgcgct gacctcccgg
     5401 aagcgccagc aggcgccaag tttttgcgcc tgccgacgct ggatcgaccg gtaacctacg
     5461 ccgaagtaga cgaaaaggtg ctgcgcatca catgccggca ttgccacgcc aaccccgaca
     5521 tcgcgctggg tgatggtggc ccaggcaaca cgggaggctt cggttttcga gcgcgcggca
     5581 tcgatctttc gagttatggc ggcgtgctgt cgggttatct ggacgaagcc ggtcaacggc
     5641 gcagcctgtt cgaaaagagc gcgagcggcg aaccgcggtt acttgcggcc ttgcttgctc
     5701 ggcacgacga agaagcgggc cacgctcagt ccgatgtgcg cggcatgcca ctgggcctac
     5761 ccgcggtcag cgccgcccag attcagctgg tcgagagctg gatcgcgcag ggtcgcccgc
     5821 gctgagggtc acgcaccacg ttggtgtcga gcgccgcgcc gtatccgcgg cgctcgctga
     5881 tgcacgacgt tgatgcccac tcaatggtgc gtggccgcga cgatcaaact caaggcgaca
     5941 ccacgcgcgt cgaactcggc ggaagaatct tctccacgta aggtcccccc caccatgagc
     6001 aaccgcccca aacctccgag gctccactgc ggtgccaccc aaaattcgta gcccgcgccg
     6061 agcatcagcc caccgccacc tcccgtccag tcttcggaag ggacgcggtt gctatctcgt
     6121 ccaaagttga ggctgccgat accgagcgcg ccctgcacgt gaaatccgtc ccgcggattc
     6181 gggtaatagt cgacgagcac gccgagcacg ctcactccac cctcttgccc ggtgagcgat
     6241 gtccgcccct cggtgaacga gcgaaggccg tcgcctttcc aacgcacctg ggaggccgcc
     6301 tgcacataga gaccgcctcc gattacgagc ccttcggcta cggtgcctcc cagcgcgatc
     6361 tccagaccta cgcccccgcc gctagcgttg agtttgccgt acggtgctgg agagtcgatc
     6421 cgcgccgcga tggtgccagc acccagccca agccgcagat agaaaccatc gtgtcgccgc
     6481 acgctcggat ctggcgctgg aaccggcgga ggcggaggtt cgagcacgat gcgtcccacg
     6541 cgcggtggaa ccaagggcgg gggcagaggt tctggcggcg gcgcaggctc gggtgactgc
     6601 gccggagacg cggccccctc ctgcggagat accgtctgag gcactgcgga cgaagcgggc
     6661 ggcgcaggtg gaactgcagc aggtggaact gcagcaggcg caactgcagc aggcggaacc
     6721 gctggcactt gcagctgcgg ttgggcgtgc gcgtgcgcgg cgtaggtcaa gctgagcgcg
     6781 agcccgatcg cgaacactct cgtgcgcgaa tcacgtcgcc ttcgcggacc accgggcggg
     6841 gtagccgctt ccgcaacaac cgcgtgcgtt cgcgactgca acgagcattg ccggagcaag
     6901 cattgctgcc aacgggcatg gctgccaact ggcctgttgc ccgctgaatg catgagccaa
     6961 accgtaacgc gagaagctgc ccacgtccac attgccacag ccgccccgaa cggccctagg
     7021 tgcggcccta cgtgtaccgg cctagatctc gcttcttgct aaacccgcat tgacctacct
     7081 ccgctcagcg gtcatttcgg gctctcctgg cccgctcgat cctccttcag cggccacccc
     7141 gccgtgattg aagtcatgaa ttctttaccg tcaactgacg agcgtagcga gctcgagcaa
     7201 cacgcgcgcg tcgcgctgga ctggcctgag ctcttggccg cgatcgcgga gcaggcccat
     7261 ggcgagcggg cgcgagaggc gctcatgacc cttcgacccg cggcaacccg agcggaagcc
     7321 ctgctacgca cggctcggct tcgaagcttg ctggagctgc aacgagccgg agtggatttg
     7381 cccgtggcag cggtgcccga gctcacggaa gccctggctc gcatacgcct cggagcgagt
     7441 gcggcgggcg ccgagctcgt aggtgtcctc cgggtactca gactggcccg agagctgcga
     7501 gagcgcctgg tcgagcacgc tgatcgagct ccgctcctat ccgagtggtt gagcaccgat
     7561 ccgaagctcg atcgtacgat ggctcgcttg gagctttgcc tggagccgag cggcaatgtc
     7621 gcggacgcgg catcagaggc cctggtcgag gctcgtgcgc gcacccgcgc ggcgcgggaa
     7681 gagctcaaac ggcgatcgag tgatctggtt cagcgctacg cggacgtgtt gtcggggagc
     7741 tactccaccg aacgagatgg ccgccacgtt ttgcccgttc gcgcggatgc gcactatcgc
     7801 gtcgaaggta tcgtgctcgg cgtcagcgcc tctggcggca cgctgttcgt cgaacctcga
     7861 gagctaaccg agctcggcaa tcgcttgcgg gtgtgcgagg cgggcgtggc ccgcgaggag
     7921 gcgcgagtgc tcgcgcagct gagcgatcta gtccgcgagc agcagccgga aatcgagtgc
     7981 gccatcgaag cttgcgtgaa cgccgacatc ctgtccgcga tcgcgcgttt cgcccaccaa
     8041 actcaagcgc tgccgctcga cgtcgcggag cgaccagtca tcgatttgcg cgccgcgcgc
     8101 caccccttgc tctcgctctc gaagcgcccc gtggtggcca atgacgtgag gctcgaggcc
     8161 ggtcaaggct tggtgatttc cgggcccaac gcgggcggca agacggtgac gctgaagttg
     8221 ctcgggctat tcgcgtggat gatccgagcg ggcttgccct tacccaccgg cgaacattcg
     8281 gagttcggtt ggttcgactg cgtgctctcg gagatcggcg atgagcagtc gctgctgcat
     8341 tctctctcta cgtttagcgc ccacgtgcaa cacctctccc aaatcctgca gcacacgggc
     8401 ccgggcacgt tggtattgct ggatgaagtc gcggccggca ccgatcccga agagggcgcc
     8461 gtgctcgcgt gcgcggtgct cgaagcgctc gccgaacggg gcggcgcagt cgtaaccacg
     8521 actcactacg agcgactcaa ggagctggcg gccaagcccg gccgcctgcg aaacgcctgc
     8581 gtaggcttcg atttcgcgca aatgtccccc accttccggc tcaccctggg catgccgggg
     8641 ccgtcgagcg ccctgttggt cgcagcgcgc tacggcatcc ccgcagagct gttgcagcgc
     8701 gcgcgcgctt tgctgcctga agcggccatc gatcgcgaag ccacgctgca ggaactcgcg
     8761 aagcaacgcg agagggtgga ccaggatcgg acccgcttgg aaagcgatcg aagagagcac
     8821 gaagcgctcg cgcgtgaact cgatcaagag cggcgggagc agcgcgcggc ggccgagcga
     8881 agcatcgaag cggaagcgcg caagttgctg cgtgacgttc agaccgcgcg cagcgagcta
     8941 tatcaggctc gcgagaagct caaacaagag cgcctggacg cggctcagct caaagcgctc
     9001 gagaactcgg tcaatcgagt ggcaagccag gtcgccatcg gagggccgtt tgcggtaccc
     9061 agccgcgctg cttcgtccca atctgcgagc ggccaatccg cgggcggcca agccgcaact
     9121 ctcgctctgg tcacacccga acgcctgcgc ccgggtacgc aagtgcgctt acgggaaaat
     9181 ggcgccctgg cgacggtact cgaagcacca caagcgggcg aggtgcgcct gcgagttggg
     9241 gcattacgca tgacccgccc actaggagcg ttgagcccgg tggctcacca cggcgtgcaa
     9301 gcaaactccg cgcccaaacg agtgcgccga gcggaactgc ccaaagcgca aatcgcgccc
     9361 cagaggaccc gcgaaaacac cttggatcta cgcgggattc gcgtggacga cgtagcttcg
     9421 caaatcgacg cttttctgga tcgcatgctg ggagaaggcg agccggtcgg gttcgtgctg
     9481 cacggacatg gcaccggtgc cgtacgccag gcaactcgtg aacaccttgc gagttctagt
     9541 tacgtagaac actcgcgggc cgcggagcct gacgaaggtg gtaacgcctt taccgtgttc
     9601 tgggtccgct agttattatc tttggtttct gcgctgccgt cgcactgttt atgcggaggc
     9661 gtgctccaac cgtccatcca accgctgcct atcgaacggc cttgatacgt ttcccgaacg
     9721 cggggcaagc cacgccgcag gcgcttggcg agcctgcatt tttactggcg ctccgcctag
     9781 cgatgctagc gtagcgccca tgccagatac ggcgtcgcgt ccaacagctt cctccgagcg
     9841 tccacgcccc ggcgccgatc ccagcccttc gagcggctcc gaaggcccag gtccttccgc
     9901 cgacagcgtc gccagcacga atgcggcgag cgcatcgtcg gcgggagcgc cttcgagccc
     9961 caacccgagc aacggctccc cccccgaacc tcgctcttcc ccgcgcgcga gctccccggg
    10021 tgcactccgc agcttgctac ccacggccgt gccgcagccg caagcgcatg cggcgccgaa
    10081 ctcgcacgcc cacggcgcgc acggtaacat gaccaaggta acgctcgccg cgctcggcat
    10141 cgtgttcggg gacatcggca cgagcccgct ctactcgatg cgcgagtgtt tccacctgat
    10201 tagcccgacg cgcgccaacg tcatgggggt gctctcgctc gtattctggt cgctcgtggt
    10261 ggtcgtctca ctcaagtacg tggtgtacgt gctgcgtgca gacaacaaag gtgaaggcgg
    10321 catcctggcg ttgatggcgc tctcgagctc cacactgaag agctcgaagt ggcacttggt
    10381 cttggtatcg ttgggcgtgt tcggggccgc gctgctgtat ggcgacgggg taatcactcc
    10441 tgccatcacc gtgctcggcg cagtcgaggg cttgagtgtg gccgccccta agctcgaatt
    10501 tctggtcatc ccgatcgcga ttgccgtact gattggcctg ttcacgatcc aacggcgcgg
    10561 cacggccggt attggggctc tattcgggcc cgtcatgttg gtttggttcg ggaccctcgc
    10621 cgtgttgggg gtgattaatc tgcacaagga cccggcggtg atctcggcgc tgctgcccca
    10681 ctacgcctgg gattttttgt ggcgaaaggg ccacatcggc ttcagcgtag tcggcgccgt
    10741 gttcctggtg gtcacgggag gggaagcgct ctacgcggat ctcggccact tcgggcgcgc
    10801 accgatccgc cgtgcttggt acgggatggt gttcccttcc ctgctcctga actacttggg
    10861 acagggcgcg ctgttgatcc accaccccga ggccaaagcc gaccccttct tcgagctcgc
    10921 gccgcgctgg gcgctttacc cgctcgttgc gctgtccacc gccgccgccg taattgcgtc
    10981 gcaagccttg atctccgggg tctactcctt gtcgcaccag ggcaccatgc ttggcttgtt
    11041 gccgcgcacg acgatccgcc acacctccgc cgaggagcgc ggacagatct acgtgccgac
    11101 cgtcaattgg ttgctgatgc tggccaccat cgcgctcgtg ctggtgttcc gctcctcgag
    11161 caacttggcc gcggcttacg gcatcgcggt cacgctcacg atggtgatca cgaccgtgct
    11221 cgccttcatt ttggtgcgca agcaatggaa gtggaacctg tggttggcga gcggagtcac
    11281 gatcggcttc ttgatcccgg agctgacctt cacggccgca aacttcacga agttttggca
    11341 tggtggtttc ttcccgctgc tcgtcggtgc gctgctgttc gtgatgatga cgacctggaa
    11401 gcgcgggcgc gaagtgctgg cccagaggtt ccgcgagcag atgttgccgc tcgaggattt
    11461 cttcgatttg atccgcgtcg agatccccgc gcgggtgccg gggacggccg tgttcatgac
    11521 cagcagtcgt gatggtaccc cgcccgcgct actccacaat ttcttacaca accgtgtggt
    11581 gcaccagcac gtcgtgctgc tcacgattac gaccgaagac gcggcccgcg tctcggagcg
    11641 cgagcgctac acgaccgaag aacttccgca aggcttcctg cgcatcgtgg cgcactacgg
    11701 gttcatggag cagcccgatg tgcccaggct gttggggcgc gcgggggtgg tgggagcgag
    11761 cctggaaggc accaccttct tcctcggtcg cgaaacgatg atcgccaccg cacgcccggg
    11821 catggcgcgt tggcgaatcc acgtgttctc tttcctgtcg cgtaactccc agccggcaac
    11881 tcggttcttc tgtatcccac cggaccgcgt gatggagatt ggcgcccaaa tcgagctgta
    11941 ggagctcgca cctgccgaat cgcacatatt tacccgggta atattgacaa gaatattacc
    12001 cgggttcaat ctgccgcgat ggtgaatgaa tctcggtcga atcgcgagca gagcgcgagt
    12061 gatgaacaac gatcgaaccc ggaccaaggc tacgtggcgt ttgctcgctc tcgccgcgtc
    12121 gcacacggct cattgcgtga ggtattgcct gtactcaaac gccgtgcgga cgccgacgcc
    12181 tcggacctcg tattagtgtt cgagctggag acggggcgcc aagtcgactt cgatttgcga
    12241 ggcactctag aggaagtctt ggatcgggag gcgcccgagc gcccccgcgg ccccgggcgt
    12301 cccaagctcg gagtcatcag tcgagaggtc tcactgttcc ctcggcactg ggagtggctc
    12361 gagcagcagc ccaacggcat ctccgcggcc ctgcgtcgct tgatcgagga agcgatcaaa
    12421 gcggctcccg gcaaagatcg cgcgcgccgg atccgagccg cattgaatcc gttcatgacc
    12481 gcgctggccg gcgatcgaga gcactacgag gaagccacgc gcgccctcta tgccggcgag
    12541 acggagcgac tagagactct gatccagcgc tggcccaagg atatccgcga gcacgtcctg
    12601 gagcgggcgc gggccgccga tctcgccgaa gcggattgac gcccgcgcca agcccattgg
    12661 tggctcgtgc aacgccgaaa gtagcgttgt catctcatac gcgcgtgcac gagagccagc
    12721 acgctccgac tcacagctcg gcgcgcgtca cgatcggcat ctctgcggcg tcagtgcgct
    12781 ggtccctctc ccaaaaccgg atcaactcgg agacggacgc ctcgacgcag atcgactcac
    12841 cttgggatag gtaaatcggg ccgctatgtg cacgcacggt atacgcggcc accaacctgc
    12901 cgtcgctcgc gtattcgccc acaccgaatg gctccggaag ctccgcagcc gggttgctgg
    12961 attggctgaa gtcttcgtat actcggcgag tgcactcggg atcggcaaag ccagtctcgt
    13021 ataggttgcc tatcgccgcg cagcgcagag tgccgtcgac ggcaggctcc acccggcacg
    13081 cgcggccgtc cggcagccag aagttcgcgc ggccgtccgg cagccagatg gcggcgcggg
    13141 gttgccgcgg agccaagtgc caaagagcac ccgacgacgg ctcacgccat acgaacatcc
    13201 tcgaccgcag ctcgccgttt cccaagggca cgacctcgag cccttgcatc gacgcgaacg
    13261 tcccggtcac gtccttgccg ggttgcattg catactcgga gggagtgctc aggggcttac
    13321 actcgggaac cgaggacttg cccgtccatt cctgatcgaa ctgcgccgcg gccggaacca
    13381 gctcatgaac ccgaccgtcg agcaccatgt aatccgcggt tccacacgca ctggggtgag
    13441 agttccaggt gtactcgctg cagtccgcat tggcgaacgg ggtattcgga gcggccagcg
    13501 gcgcaggcac gcaatcggcg ctctcagcca gcacacacga tcgatggccc gtggtcgtga
    13561 gcgaccacgt aagctctgcc ccatcttcca tttgaactcg cgtgaggccc aggtgttcgt
    13621 gcaagtctat ggatctgagt tgcccaaaca ccaactcgga gtcaggaatg ggctccaagc
    13681 ggtgcactgg aggatcgatc ttgcccatgt gctcggcggg cacacagcct ttcgcgctca
    13741 ttttgaagat gtcgtgggtg gcctgcgacg gcggcaaggc ttcctcgtaa acccgctcgc
    13801 ccaagacgta ggcgctattc gcttccggaa gatcccccgt acagacgttg gagtccgaag
    13861 gcccctcggc ggttacggga tcacccactt ccgcgtcgcc cggaaaccag gtcgcgggtt
    13921 gagtgcattg cgcgtcgagg aacaacaacc gggcggacct cgcgggcaaa caaaccacgc
    13981 gatcgcctcg actcgcgaac tgacaaggtc cccattcggt gtcttcgaaa ccagtgaatg
    14041 agtaagcgcc gtccgcgacg aagcccgtgg ctttgagccg cttgcccgac ttgacgtacg
    14101 gaagcggctg gggccccgcg ctagacccac cccagccgct tccaccgttt tggacttcgc
    14161 cggcggcgcc agcttggctc accccgccga tttcgccgcc ctgggctccg ccctcgctct
    14221 tggaaccgcc cttgctctcg gaaccgccct tgctctggaa gccgccgtcg gcctggcccg
    14281 cgccgccacg gcctgcttcc caggcggtgt ctccacccat catgccgtgg gtgcttcccg
    14341 cgctcgaccc gccggtacca cccgcatcga tgctcttcgc gacgtttccc gagcagccac
    14401 agagcaggcc aaccgcaaat cctgccaccc acggactacg cttcggcgta gccaagctaa
    14461 ttccttctcc caaacccaat ttgttcgagt gcatctcagt aaggaatagc gattggaagc
    14521 cgctcaatcc aatcgagaga ttctatggtt ctccatgcca tttcctcatc gatgaaaaac
    14581 ttgcgagcgt tgcggttgca gctgctcagg gttgcagccg ctcagggttg caaaagctca
    14641 tgtagcaact gctcggctcg ctcacgcacc agcggttcgt cttgctgcgc cgcaaactcg
    14701 agcttggctt gagtaaagcg ccccagctcg ctggtggtct ccgccagggt cggcgcggcg
    14761 tcgcgcgcca acagactcag aactctcgac tcttcggcgg gagtgaggac ttccgagtga
    14821 gccacgatca ccggggccgc ccgatctacc gggctcgaga aattcatggc gatcggtgag
    14881 gcggcagcgt ccagcatata gatcagccgc aggccgggtg tttcgaggaa cggaatcgac
    14941 ttcgactcgg cgatcgtggc gcccagctct tcacccaagc gctcgacgaa gaagtccttc
    15001 aacgtgtccc gggcccgctc gagcagtgca gcaatgggct gttccttggg gcccaaggtc
    15061 gtctcgcgcc gctcgccggg cccgagatcg agcacctcgc gcaccccgac cccttccgag
    15121 tgtgaataga tgagcagggc ctgcgcgatc gtctgattcg tgggattcga gatgatcaga
    15181 tctcgcgcgt ccgagctcaa gcgcagcggt gaaaacacgc cgcgtgcggc aaagctcaag
    15241 atggctgcgg gcgcgctcgg cgccgggttt cccggcgcct gattgctgtc gtcgtcgacc
    15301 gctcggaccg cgaggccacc ccaagctttc catccggtcc attcggtggt gcggagccaa
    15361 tcggagctca ccgtcgagtc gtcgggctct gagagcgcca acccccaaac cagccctccg
    15421 cgatcggcac gcaacgcggg gaacccccat tccacgggcc aggaccgttg agcgctgaaa
    15481 atcggtgaca ccgagagggc tccgccgcga acatcgccag cggccgcgcg gaacacgaga
    15541 gcggtatcga tttctgcccg atcctcgagc cctcctgtgg acgactgctc ttgcgcggct
    15601 cgttctcgct ccacgaactc cggcagctgc agctccggct caggcgctaa cgcaagcccg
    15661 gcggaggctt gcaccgtcgt caacgtggtg acctgcacca cctccaggcc tggctgactc
    15721 gtgcccgcgg caccagcatc ggcggaactg gcatcggcgg aactggcacc agcaccggca
    15781 cccccggaat tggcaccggc gctcgatgcg ctcgtcgctc cacccgtcgg tgtagtctgc
    15841 gatgccccgc cgtttcccgc gatggcggcc gccgtgccag gcgcgtcatc ttcggcgcaa
    15901 gcagccggaa ccagcagtcc acacaacacc gacaacagcg cccgcgatgc ccgcgaccac
    15961 cccgtcgggc caaaagaaag ggtccgattc ggggatccgg ccccgaaaag cgccaggcgt
    16021 gtgcttcgca tcgcgctgca tagtaacact gcttgccggg tccgccctat gcaaccttgg
    16081 catggttacc catagaagtt ttgcatgaac ctcgagcggc tagagctttt cttgagaatc
    16141 gtagatacgg gctccgtaag cgcggcgagc cgcgaagccc acctgacgca acccgccgtc
    16201 agccgcagcc tgaagctcct cgaggaagat atcggcagtg acctgttcga tcgtcaaggg
    16261 cgcgggctat tactcaccgc ggctgggcgc gctttggtcc cctgtgcgag acagctggtt
    16321 cgtgaggcgg agcgggcccg cttgcaggtg cagcgggcgg cccagagcgc atgcttcgat
    16381 ttgcggctgg gcgcggtcga cagcattggc acccacgtac tgccgaattg cctgatggcg
    16441 gtccaggagc gccaccccga aatcaagatc aagctctgga tgggcagaac acaaagcctc
    16501 ctcgaacgcg tcgaacgggg aacgctcgat ttggctctcg tagcctactc cggtccgccc
    16561 tccgagcgag cccaacgcat tggcagttac gggctcaagt actatggcca ccggcagcgc
    16621 tatccggagc ttgccacctt gtcgtccgag ctagagctgc gccgcctgcc aatcgtggaa
    16681 atcgaggcct tgcccggaca gcctacgctg attcaagatg actcggatgc gttcgcggtg
    16741 actcagaatc tgagcgccgt gaaggcgctc gtgctcgccg gtttgggagt ggggcagctg
    16801 ctgcccttca tgctcacgcc ggaggaacgc gaccgcctgg tgtgtgcggg ttcgattcga
    16861 catgatccgg attgtgggat ctatggagtg cgatcgggcc gcgcgctgac gaccgtcgag
    16921 accgccatcc aggacgattt actcgccacc ttgaaggcgc agctagcccc cctgcccacg
    16981 gacgagggca gcctgggcgg cgcctattga gaacgccgtc ggcgctcgag ataagcagga
    17041 aatcccgccc agttcggcaa accacgcacg aagtttggcc aggcctgctg gagcgcgtct
    17101 tgcatccgtt gattcagatt gtggccggat aacggatctc ccgcgacttg aaattcaatc
    17161 cccgcccgag agacgtggct ggccgccgac cgaagagtag caaaacaagc gttggagccg
    17221 cagatcccca acagccgaga ggtgccttgc ccgtgcaggc gatccaagaa gcccgcgtct
    17281 ctcaagagat tgtatccgcc ctccgccaac gccacgttcg ggaagcgccc cggctccgcc
    17341 agcaacgctg cctcggcgag cgtcgctcct tgactgaatc ccacgtatag agttggcccc
    17401 tccgccacgt gggttccata acgctgcttg actgcctgaa tcgccaaccg cacgcgctcg
    17461 gcgatcgtgc ggggggcatc ccaagcgaaa cgttgagaac ccatcggcag tcctcgcgga
    17521 cacaccacga acgcgtactc actggcggcc agtctccagc ctccacacgc ccactcgggc
    17581 cgatctcccg ccccgtggac acccaccatc agcggacgcg gctcgcgcgc tcccaatgcg
    17641 ggcgtcacgt aagcgagctc tcccccttcg agtggcaacg gctcgagcca gctgacgtcc
    17701 cgcaacgggg caagggtggc ctggtcttcc gacgtcatag cggaaggcat cgtcttcgcg
    17761 tctcgaacgc tcggcgcttg cgtgccctgt ccatgcaatc cggcggtggc ctcggcgggg
    17821 gtagcaggag cgggctgctc cagcgcaact gcgtcgcggc ccgacggtga ctccgacgat
    17881 gatggctggc gacaagcaac aatcaccaag cagccgagcg gaatcgagag ttccttcatc
    17941 ctcgacccaa ctcgaactca cgcgccaaca gctcgtacga tcttagtcgc tgctccggga
    18001 cataagcgtg cgttgccacc atcacctcat cggccgcagt gctctgggcc accgcctcga
    18061 tccgttcccg tacgtaagcc ggtgtaccga cgatttgcaa acggcgtgcg aatccgatcg
    18121 cggcttcctc ttccggtgag aatcgatatt gctccgcttg ctccggactc atcagtcgcg
    18181 agggccgccc tgcgcgtaat tgcgcgaacg cgaccagcat cgaagaggcc aatcgtttgg
    18241 cggccacctc ggtttccccg cagaaaaccg acagcgcgac gattgcatgc ggctcccgaa
    18301 acgggcctgg ctgaaaagac tctcgataca actgaagcgg ccactccggc ggctccgggc
    18361 tgaaatgacc cgcgaacgca aaacccagtc ccagctgcgc cgccaagcgt gcgctgtagt
    18421 ccgaggaacc caacagccag attgggggca gcgtaacgtc gtccggcatc gccacgattt
    18481 gggaaaacgg gtgcccagcg ggcaaactct gggtcccgaa cccccgcagc tccgccagca
    18541 aaccggggaa ctcatcgctc cctccatatc gaccgcggcg cagtgcgctc gcggtcaccg
    18601 gatcggtacc aggggcacga ccaattccca agtctatgcg cccgggatgc agcgcctcca
    18661 gcaagcgata cgcctcggcc acttggagcg gggcgtgatt cggcagcatc actccacccg
    18721 cgccaaggcg aatgcgttgc gtgcgcggcg ccacatgggc gatcaagagc gcgggcgtgg
    18781 tggtcgcgat cccgggcatg ttgtgatgct cggcgtacca aactcgctgg taaccccaat
    18841 tgtccagagc gcgcgccaac gctaccgaag catgaacggc ggcgtggctc gaatagccgc
    18901 tgcccagagg gaccagatcg agcgcggaga gcggcaaaac catccgcaga gcttgaggcc
    18961 aacccgcggc cacgcaagcc ggtcactagc caatttgcca gctcgcgttc gaggctccaa
    19021 gcatgatgtt agcgtgtacc gatgcgcgta acgcactcgc tggtggccac cacgattgct
    19081 tgcctggcga gcaccgcaac atagggctcg attggggcga tgcagcagca cgacccgctg
    19141 ttgccgcagc tcgcgtggga gagctggtgg cttttcccca tctccctact ccatcgggct
    19201 gctgttcggc aaaggaacac tctggttctt ccggaccgcg ccgttgcaga cgatcgagca
    19261 atggaaaggg ccgcatcagg agagtatcga tatccgagcg cgcggcagcg atccggctgg
    19321 ccttcagggc tagccgcgga gccaagcccc tagagtgccg cgttcgcttt gaccccatcc
    19381 caatacgagc tagcccgccg tcgctctact gggatgtgct tcaaggccta ttcgccacac
    19441 gaccgcgaaa tttttctggc cccgcggttc caaatcgctc tcgaggccct gcgcaacatc
    19501 agaaacgcgc ccactcgggc cataaaaggc ttggaagaac ggccttgcag aacgattatg
    19561 ccccggccag agcccgtgga gcgctggcac ggaagcgcga ataggtaaac agaaatgaga
    19621 catcgaattt acggacaggc gatcgattcc gtcggtcgtg cagcgaccag agcattgctc
    19681 gtgtgcgcct cggccgccac actcgcatgc atccctccac tcgaggaacc caagacggct
    19741 cagggagagg ctgcgcaggc gccaccgtcg gatgagctga cgcgcaaggg cctcgatgcc
    19801 atcaaggcca aggactacgc gaaggccaag cagttcttgg cggaagcacg caaggcgaac
    19861 cccaaggacg cacagaatcc gtactacctc ggagttgcgg agcaagagtc ggggaaccgt
    19921 gccggggcga ttgccgcgta tcgggagtgt ttggcgctcg atcccaagtt caccgaagcc
    19981 agcatcaact tgggtgcgat tctgctcgag ggccacaatc ccgccgaggt cgcggagtcc
    20041 gtcaaagtct ccgacgaagg cctgaagcac aacccgaagt ccgcggatct ggccaagaac
    20101 aaagccgtcg ccctcgcgga tagcggcgac ttcgccgccg ccgccgaggc ttacaagaag
    20161 ctcatggaag cggcgcctca ggacctgcgc ctccgctacg actacgctcg catcctgagc
    20221 gacagcggca agatcccgga agccattgcc gcgctcaaac cagtcggaga aagcaccgac
    20281 gtgaagctgc tcaacgcggc aggcagcctc aacgcccaac ttggcgctta tgaggaatgt
    20341 gtcaccgtgt tcgataaggt catccaagcc gggccgaacg tgtccgcgtt ggtcaatcgt
    20401 ggcaattgca agcgtggact caaagatctg aaggccgagc gcgcggatta cgagcaagcg
    20461 ctgcaaatcg acccgaacta cgcccccgct cacttgagcc tcggcttgaa cctgttctac
    20521 gtcggcaaag atcgcgctca agccattgcc gagctgcaga aggccaaaca actgggccaa
    20581 ggcacgcccg tggccgaaga agcggacaag atgctcgccg agctggaccg cgcgagcggc
    20641 aagaaaaaga agaagtaaac tcgcccgagg caccggcctc ccgtgcaccc aacggtttgg
    20701 aatcagccgc gcgagtgttg ctctagcgag tctccaaacc gttcggtgct cccgcgtgca
    20761 gcgtttcccg gctcgcgttt agccccttac ccgaagcctt gcggatgcag agtcccgtgg
    20821 agcacgctga gcgtccaaga acgcttcgcc accgctcgaa cgagcaacct gctagacgac
    20881 tgccctcggg gctacgctat atagagcgcc gcagtgccga atccggagct cgaacttcgc
    20941 tcgttcagcc accaagatcc cgccgatcgg gagttcgtat cccagctctc ccgcgaggcg
    21001 ttcagcgaat tttcgttgaa cgcgcccagc acgaccctgc acatggccac ccacgaagcg
    21061 agctggattg cgctcgaatc gggcacgcgc gtcggtctcg ccgtgtgtgt gatccagcca
    21121 tccgaactcg cggagctctg cgccatcgcc gtgatcgctc gagagcgggg gcggggcatc
    21181 gggcgtgctt tgctctcgca cgccgagcgc gccacccgcg cgcgaggagc gcccggtatg
    21241 ttgctccaca ccgcgaccgc caacgtggcc gctctcgatc tcttcttgcg caacggatat
    21301 cggatccggc gccgactccc ccgctactat cgaaacgtct acgaagcctg tgagctcgtc
    21361 aaacactggc gaagaatcta gacactcaga tggatcgcgg gggtcgggcg gggcggcctc
    21421 atccgcaaca tcgattcgtg caccaccaac aatgcggcgc tcgcgacgac gatcagcaga
    21481 ccaatcgcgc tcaggccgag gtgcccaacc aatacccccg tgagactcgg caccagcgaa
    21541 gaccccagcg tcgccgcgct cacctgaaag cccaccgcgt gcggagtgac
//
LOCUS       DASTKU010000097         4765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_465642, whole genome shotgun sequence.
ACCESSION   DASTKU010000097 DASTKU010000000
VERSION     DASTKU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465642"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4510)
                     /locus_tag="VFQ61_03465"
     CDS             complement(<1..4510)
                     /locus_tag="VFQ61_03465"
                     /inference="COORDINATES: protein motif:HMM:NF023677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
                     /protein_id="HET9953533.1"
                     /translation="MDGDGFTDWISPGGTVSRNDRRGALTPLGRSINGLPGFLQDSVR
                     NLLLEGAQHADPLVRWLAPYSGTVAISGPIARKESGGGDVLAEIWRDGQDAPRWSRVF
                     AASDMNPCQPGAASGSCDGDLTQYVNAGDALYFRVQAMGDMEHTALLWDPRISYRTFC
                     QIGTCTELGEADHAQREPWGAPVYDFSQAEDFRLAGPPHRMWTTSSFGTANVSLDDAV
                     FTKRAPTADDVTVRVIHHDAHSGVDKDLFVQTFRAAEVASVDVGSALRAAQTAISVTA
                     SATTDATGAPSEILADGLRFEVFSDSPIDPNALSWSPKVRYDSYCRTPPQENATPVCG
                     AVECSQVGDGSPCTMQNDPSPESPLVRALIVQAAPVTYSNYLVKGATDNLAVSENVPT
                     RAWPAPETGTFQVTVTYALASSAATAGTDDTVFLVQGNQKLLAKFIIRAGTSGTQVLT
                     GLAATAGEPLYFTALRKGSQEFTWSVEVRSETSGTNYGFPVFRQHRVSAWDDAGEFGM
                     MSGGYHQWSTGQWTHTSGASPVISAAAIENRKQPTEGDAANRPFYATFPSPRGVAPNL
                     SQLVPGSSLPAWTSSGLDFYISAGELKPSYVGSNPRALIDAAQAPAVRGSHGSSDSGS
                     VDLTVGGFTKTGSESEGDVDLLDLNGDGYPDSFNTSSVSYGDGQGSFTPNQSRDFGGG
                     FRKFKSNHIRYGFAAGTAAGAIAMNLGASGVVDTIAHLLPSFGRTYGRTRATLDLIDV
                     NGDGLPDRVERSDLRADAQVRLNLGYGFGPAFPLAVPRFSSTIGGEGIPDFTPESGEE
                     ASLQLQTNIANSYQMGHAGIGGGLAYSSTRTLIDFADVTGDGLPDRVLKIPGRPQLYV
                     QINTGEGFAPEQVWAMPDWGQELPSSGPLLGMGSRDVLAFSEGREWHLGAGGAGEIPV
                     WLGICLAAEISAQFVHNSLRSELAFQDVDGDGFADHVLKTEGQGQVYVKLNEQGAQRY
                     NLLASVTNPLGGRVFLDYQRVGNTVELPQSQWVLSRVTHDDGLDGAFHDTPRTTRFEY
                     SGGLYHRDERQNYGFSQLITRRPDESVVVQSFRQDSACSRGVVWKTLEQDAEGKLSRK
                     HQTVFEVRTSDTLDSCFAAATDEVTSLYDGVTKDENQPSLTLEEQRDYDQFGNVTFYS
                     NAGDSGAEDDLSHTVDYDPVLVRSGIFRPTNVTAFDSRGVMLRERVATYHATGDLHTL
                     HNIVTGGHQLDTGEPYQAQATPDWVFDYDAYGNTATVTDPTGFYVQNTAFDAETHTYP
                     TTTQDAFGYSSSAVPFPQLGTNTSVTDIRGNTVKFYYGRDNLVVPRVLSVQAPTELAA
                     GVDTVSVEYFLGGEGGPSRAIVRHFDSEHPTDPLETVTFVDGFGRTIQVKKETDVDDG
                     SGGVTHGFTVSGLTLYDERGRVLEQGQPVFNAGPANDLVPVALSNPTRYEYDVLSRPT
                     KVTQPNSAVVSTEYGFAPFDGKTRARTKLTDPNKYVRVTYEDVRGQTVGIEQVNTIDG
                     SDKTLTTRY"
BASE COUNT          894 a         1507 c         1431 g          933 t
ORIGIN      
        1 aatagcgggt ggtgagagtc ttgtccgacc cgtcgatcgt gttgacttgc tcgatgccga
       61 cggtctgccc ccggacatct tcgtaggtga ctcggacgta cttgttggga tccgtgagct
      121 tagtacgcgc gcgcgttttg ccatcgaacg gcgcgaaacc gtattcagtg ctcaccaccg
      181 cgctgttcgg ctgggtcact ttggtcggcc gagataaaac gtcgtactcg taccgagtcg
      241 ggttactgag cgccaccggg acgagatcgt ttgctggccc agcattgaat accggttgcc
      301 cctgttccag cactcgcccg cgctcgtcat agagcgtgag gccgctcacg gtgaacccgt
      361 gagtcacgcc gcccgagccg tcgtcgacat cggtttcctt cttgacctga atggtgcgcc
      421 cgaagccgtc cacgaacgtc accgtctcga gcggatcggt cggatgctcg ctgtcaaagt
      481 gacgcactat ggcgcgcgac ggcccgcctt ctccgcccag gaagtactcc acggataccg
      541 tgtccacgcc cgcggcaagc tcggtgggcg cctgcacgct caggactcgc ggaaccacca
      601 agttgtccct gccgtaatag aacttgaccg tgttgccgcg gatgtccgtg acactcgtgt
      661 tcgttccgag ctgcgggaac ggcaccgcac tcgaggagta cccaaacgca tcttgggtcg
      721 tggtcggata ggtatgagtc tccgcgtcga aggcggtgtt ctgcacatag aacccggtcg
      781 ggtcggtgac cgtggcggtg ttgccgtagg cgtcataatc gaacacccaa tcgggcgtgg
      841 cctgtgcctg atagggctcg cccgtatcga gctggtggcc gccggtaacg atgttgtgca
      901 gcgtatgcag gtcacccgtc gcgtggtagg tggccacgcg ctcgcggagc atcacgcctc
      961 gactgtcgaa ggcggtaacg ttggtggggc ggaagatccc cgagcgcacc agcaccggat
     1021 cgtagtcgac ggtgtggctg agatcgtcct ccgccccgga gtcacctgca ttgctgtaga
     1081 acgtgacgtt accgaactgg tcgtagtcgc gctgttcctc cagtgtgagg ctgggctgat
     1141 tctcgtcctt ggtgacccca tcgtacaacg aggtcacctc gtcagttgcc gctgcaaagc
     1201 aagagtcgag cgtgtcgctg gttcgcacct cgaacacagt ttggtgcttg cgggagagct
     1261 tcccttcggc atcctgctcc aaagtcttcc agaccacgcc gcgcgagcac gcggagtctt
     1321 gtcggaagct ctgcaccacc acgctctcgt cgggccgtcg tgtgatgagt tgcgagaagc
     1381 cgtagttctg cctctcgtca cggtgataga gtcctccgct gtactcgaag cgagtcgtac
     1441 gcggtgtatc gtggaatgcg ccatccagcc catcatcgtg cgtcacgcgc gagagaaccc
     1501 attggctctg cggaagctcg acggtgtttc cgaccctctg gtagtcgagg aataccctcc
     1561 cccccagtgg attagtcaca ctcgccagca ggttatagcg ttgcgcgcct tgctcgttca
     1621 acttgacgta aacttgtccc tgcccctccg tcttgagcac atgatcggcg aagccgtcgc
     1681 catccacgtc ttgaaacgcc agctcgcttc gcagcgagtt gtgaacgaac tgtgccgaga
     1741 tctccgccgc aaggcagatc cccaaccaga ccgggatttc gccagcgcca cctgccccga
     1801 gatgccactc tctgccttca gaaaacgcga gcacgtcgcg cgagcccatg cccagcaagg
     1861 ggccactgct aggaagctcc tgcccccagt ccggcatggc ccacacctgc tcgggagcaa
     1921 agccctcgcc ggtgttgatc tggacgtaca gttgcggccg acccgggatc ttgaggacgc
     1981 gatccggcag gccgtctccg gtcacgtcgg caaagtcgat cagcgttcga gtgctcgaat
     2041 acgcgagccc tccgccaatt ccggcgtgcc ccatctgata gctattggct atgttggtct
     2101 gcagctgaag cgatgcttcc tcgccagatt cgggagtgaa gtcaggaatg ccctctcctc
     2161 cgatggtcga ggaaaagcga ggaacggcta gcgggaacgc tggaccgaaa ccgtagccta
     2221 ggttcaaacg cacctgagcg tccgcgcgca gatcggagcg ctccacgcgg tcgggaaggc
     2281 cgtcaccgtt gacgtcgatg aggtcgagag tggccctcgt gcgcccgtac gtgcgcccga
     2341 aggacggcag cagatgagca atcgtgtcca cgactccgct cgcgccgaga ttcatagcga
     2401 tggcgcctgc cgcggtaccg gctgcgaaac catagcgaat gtgattggac ttgaactttc
     2461 ggaatccccc tccaaagtct ctgctttgat tcggagtaaa gctgccttgt ccgtcgccgt
     2521 aactcaccga gcttgtgttg aacgagtccg ggtaaccatc tccgttcaga tcgaggagat
     2581 ctacgtcccc ctcagactct gaacccgtct tggtaaatcc cccaacagta agatcgacgc
     2641 tcccactatc actcgaacca tggctaccgc gtaccgcggg ggcctgcgca gcgtcgatca
     2701 gcgcgcgagg attcgatccc acatacgacg gtttcagctc tccggcggag atgtagaagt
     2761 cgagtccgct agacgtccac gctgggagcg aggagccggg cacgagttgg gacaggttcg
     2821 gagcaacacc ccgtggagag gggaacgtgg cgtagaaggg gcgattggct gcgtcccctt
     2881 cggtcggttg cttgcggttt tcaatggcgg ccgcggagat aaccgggctc gcgccggacg
     2941 tgtgcgtcca ttgccccgtg ctccattgat ggtacccgcc cgacatcatg ccgaactcgc
     3001 cggcgtcatc ccatgcggac acccggtgct gccggaagac aggaaatccg tagttggtgc
     3061 cagaagtttc cgaccgaacc tccaccgacc aggtgaactc ttgactgccc ttacgcagcg
     3121 ccgtgaagta gagcggctct cccgctgtcg cggcgagccc agtgagcacc tgagtgccgc
     3181 tcgtcccggc tcgaattatg aacttggcga gcagcttttg gttaccctgg accagaaaca
     3241 cggtatcgtc tgtacccgcc gttgccgcgc tcgaggcaag agcgtacgtg acggtcactt
     3301 gaaatgtgcc cgtctcggga gcaggccagg cccgcgtagg cacgttctca gaaaccgcca
     3361 gattgtcggt cgcgcctttg accagatagt tagagtaggt cacgggcgcc gcttgcacta
     3421 tcagggcccg cacgagaggg ctctcgggcg aagggtcatt ctgcatcgtg cacggcgagc
     3481 cgtccccgac ctgggagcac tccaccgctc cgcaaaccgg cgtagcgttc tcctgcgggg
     3541 gagtacggca gtagctatcg taccgaacct ttggactcca actcaatgcg ttcggatcga
     3601 tggggctatc cgagaacacc tcgaatctca gtccatccgc caagatctcg ctcggagctc
     3661 ccgtggcatc ggttgtcgca gaagcggtca ccgagattgc ggtctgagcc gcgcgcaagg
     3721 cgctccctac atcaaccgat gcgacttcgg cggctcggaa tgtctggaca aacaagtcct
     3781 tgtcgacgcc ggaatgtgcg tcgtggtgga tgactcgcac cgtgacgtca tccgccgtcg
     3841 gcgcgcgctt cgtgaacacc gcatcgtcca acgagacgtt cgctgttccg aacgagctcg
     3901 tcgtccacat gcgatgcggc ggcccggcaa gtcggaagtc ctcagcttgc gagaagtcgt
     3961 aaactggtgc gccccaaggt tcacgctgcg cgtgatcagc ctccccgagc tcggtgcaag
     4021 tgccaatctg gcagaacgtt cgataggaaa tgcgcggatc ccagagcagg gctgtgtgct
     4081 ccatgtcgcc catcgcttgt acccggaagt acagcgcgtc tccggcattg acatactgtg
     4141 tgaggtcgcc atcacaagag cctgaggcgg ctccgggctg gcaaggattc atatccgatg
     4201 cagcaaagac gcgcgaccat cggggtgcgt cttgcccatc gcgccagatt tccgcgagca
     4261 cgtcgccacc gcccgattcc ttccgcgcga tcgggcccga gatggccacc gtaccggaat
     4321 agggcgcgag ccaacggacg agcggatccg catgctgagc gccttccagc agcagattcc
     4381 ggacggaatc ctgaagaaag cccggaagac cattgattga acgcccgagc ggcgtcagcg
     4441 cgccacgtct gtcgttccgt gacacggttc cacccggtga gatccaatcg gtaaagccgt
     4501 cgccatccac atcagcgaag cctccctttt gtttcgtttc agtccacgac caggtgtgac
     4561 ctcctttccc aatctccccg aggacgtgaa gcccagcgct tcgactatgc gtatagtgct
     4621 cagtcgagaa gaattcgttg cccaagcccg ggatctgcgc ggatcgataa ccgcccgtta
     4681 ctgaaccgaa aagtccgcca ctcagatagt ctggcaatcc atcgccgtcg gcatcgactg
     4741 acggaacgag tgtcgcacgt ccgcc
//
LOCUS       DASTKU010000098        27867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1580404, whole genome shotgun sequence.
ACCESSION   DASTKU010000098 DASTKU010000000
VERSION     DASTKU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 27867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27867
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580404"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFQ61_03470"
     CDS             complement(<1..703)
                     /locus_tag="VFQ61_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953534.1"
                     /translation="MRSVIPANVVRSDTTLAYYEGTNGRTGSTIASTLLASMKLSDEF
                     AAFARFAAVSNSPPSGEPGSSFVNPILGGLYGVKLGSALRFAATLGVALPLGNGGGNT
                     PNAQRAAANAAGIRARSAMDNALFAVNDLVLFPGVDLAFIQGGFTAQIEATLLQLTRV
                     RGEDKQPDSSRTNFTSGVHVGYFLIPELSLGVELRHQRWLSTPKAVSAAPATRDTTTF
                     AVGPRVHLKLAEKVWL"
     gene            complement(1406..2320)
                     /gene="gcvA"
                     /locus_tag="VFQ61_03475"
     CDS             complement(1406..2320)
                     /gene="gcvA"
                     /locus_tag="VFQ61_03475"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HET9953535.1"
                     /translation="MGDSRAPLPSLNALLAFESAARHGSFTRAAKELKVTQTAVSHQI
                     KSLEAELGVSLFRRLPGRLELTREGQAWAAELSVVFQRLYDANDKLRRVAAYERQMVA
                     ISSIPSFCARWLVPRLGKFMALYPDIDVRISATEALVDFSREPFDLGIRYGSGGYTGL
                     VSEKLYADHFVLVCNPALHRKRRRWLANDLLRETLITDDHPTALEHWLTRAGLRDLSR
                     ARCHRISDSSMLVEAAIRGQGMALARFSLAADELAAGHLVLPFPEAEPLPSGFAYYLV
                     GPRENLRRPCVARFRDWIREQVQPLLEA"
     gene            2390..3043
                     /locus_tag="VFQ61_03480"
     CDS             2390..3043
                     /locus_tag="VFQ61_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HET9953536.1"
                     /translation="MKLFRLRYSPYARKVQMLLDLIPRRYESIEVSYGDRNELAELTS
                     GYIYVPVLEDGGKVIVDSRRIAQHLLSDPNAAHLVPAPHQGPIWAYADFADGPLEDVL
                     FRIASPFIRDQWQTPFERALYTLIKERKFGAGCLDAWAAQRDELFARGRELLEPTLLT
                     LGQQPFLFGQSPTLADAALYGLCEMLEEAKPELLAQLSPRLPEYARRLERFAKERVR"
     gene            3128..4204
                     /locus_tag="VFQ61_03485"
     CDS             3128..4204
                     /locus_tag="VFQ61_03485"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HET9953537.1"
                     /translation="MLVSRRTLLASTIVSGGAWIGAGALLFTGSGCRKKPAPPGPERL
                     YVSTEEGGEVVVLEATTLAIVARIPVGKRPRGLKLASTGSELYVALSGSPRSGPGADE
                     SKLPPADRRADGIGVVDLTVRKLIRTLESGQDPETFDLSKDGQRAFISNEETAELSVL
                     DLKSGHVVSRVAVGDEPEGVSLRPDGAVVYVTSEADGGVSVVDTKTLKVVAQFTTGPR
                     PRVIAFTKNGKQAFISNELGGSVSVVDAVAHRVTGEIPVTGPGPAPHRPMGLVLSSDE
                     GQLFVSTGRGGAVAVIDVLARKVSRWISGVGARPWGLTVSRDGKRLYTANGPSNDVSM
                     LEVATGQVLKRAEIGGLPWGIIAG"
     gene            complement(4313..5620)
                     /locus_tag="VFQ61_03490"
     CDS             complement(4313..5620)
                     /locus_tag="VFQ61_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9953538.1"
                     /translation="MKPPKLNLGKELHVSVGAVAGGTGTEAQPFASLKAVLDAIAQDD
                     DWTGSIVMHPGKHSVTEEAVLPKTATWKLMPGTTIAAGPGVSIHAQNDVDARGTESSP
                     ILFTWLEKDKHWGSLTNFTKGSDNNVFEYVTFEHGYESDFEGVGMRGALSLKNAKARI
                     SHCQFINNEGDDGLNLKATDTLVEYSLFKGNASDGIDSDGVGKPEIRFCTFDNQINDN
                     LDLGEGTSAFVHHNLIMGGQDKGISNGDGATPTLENNLIVNNFLGIGIKDDADPMVRN
                     NTIYGNQYGIRLYHHVDDYGNAKGTVVNNIIWGSALQDVMYEAGDTVFKYNCIGSLKT
                     EEGEDVIDNTGALLADMEGILTVGTGCDDPGFVDPANLDFHLKSEAGRWDASSESWVK
                     DKESSPAIDRGDPDQDVGDEPAPNGGRINLGAEGGTEEASKSP"
     gene            complement(5920..7392)
                     /locus_tag="VFQ61_03495"
     CDS             complement(5920..7392)
                     /locus_tag="VFQ61_03495"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9953539.1"
                     /translation="MPRVTAKIDDISKRRTLKLHRVSTISTLFMTTLSGLMLACGGGE
                     PASGQPQTESPNPPSEVIGGCMGDCGPSTRDFFSNEQVADLKLTIDDATLKSYGYEPG
                     QWLDLLWDKWNNHCGPYDWVPVTMTYESPDGNGNTTLEKVAMRLRGTKSRGVNRLQGF
                     KLDFTKSLPKDNNRRFAGLSRVNALSNEGDDSNMLQCMGYKLMRDFGLEAPLCNHLRV
                     FINGKLYGVMESVERAKDARFLKNHFETSNGFLYAASASCGYGDSLADLGYKGDTFDV
                     PDYTKIYEVLRGTPQDAEVNLIPMLKCGDPAQTPDDEDFKTCISEWIDVEQWLREIAA
                     ESIMPSLEDFVGARRNYFLYFKPDAAAPHGGRMLVWGWDYDTVLQRATCYPQSCDPFT
                     SVAGWFGPRGKRQPLLTRLTSVFKARYCELLNEYLDEVYDPTKVDEMASVIEGSMAED
                     TVTEFSAWQSEVTRMREYMVKHAGDARLQIAKNCEMTASE"
     gene            complement(7987..9075)
                     /locus_tag="VFQ61_03500"
     CDS             complement(7987..9075)
                     /locus_tag="VFQ61_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9953540.1"
                     /translation="MRRAEFSMDRAQAREFLARAELIRLATSTPEGEPVLRTLNAVVL
                     DDWLLFHGAKAGEKARCLGRTAVVSADELLATVPSYFTDPERACPATSLYESAMAQGT
                     LESIQDGELKARMLTALMQKYQPEGGYAPIDHTSEMYRGAVRGVLVFGVRLDRIAGKL
                     KVLQNKPPEVIRQVIEGLWQRGGVRDVHAIQRIFGGNPGLERPARFMIASHGLRLEPE
                     LTEQDWAATFALIRDQYWNRDYSQQELRRAHLESSAWVGVRSASGELVGTARAVSDNS
                     KHTYLADVVIEERQRGRGIGRRLIEFLLEHPKVRASRVVRLGTADAQPFYRKLGFVPP
                     SEVDFGFSSTPMLLVRPSSATIGRSPAL"
     gene            9141..10646
                     /locus_tag="VFQ61_03505"
     CDS             9141..10646
                     /locus_tag="VFQ61_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9953541.1"
                     /translation="MEYVPNLLDPHEPEATASAAPLFARIAQAIAEDIRRGRLRPGRR
                     LPGSRQLALSLGVHRNTALAAYTELERQGYIVTRRARGTFVAEALPEVRPRKLRPSAA
                     RHHDSVLSLPPWPKALIPRLPEQPRFSAKSLPLLAGMPDPRLMPRGALSRAYRRVLAR
                     EPDVLNYASPQGHPRLVRALCEQLRTTRGVLASENEIVITRGTQMAAFMAALALCSPG
                     DRIAVEALGYPPVWQAFRLAGAELVAVPVDGAGMRVDKLEALLQTSKIRAVYVTPHHQ
                     YPTTVSMTAARRLQLLALAERHGFAILEDDYDHEFHWSGRPLLPIASVANAKCVVYLG
                     TLAKVLAPGLRIGYMVAPEHVVERILALRRFIDRQGDHAVEAAVAELIEDGEFDRHAK
                     RTRRAYLERRETLIEGLKREFGERLHYDLPHGGLGLWLRADLPMGAQRFTELAAEQQV
                     HLQHGGQFSFDGRASEFLRVGFPRLNQTEIERALRILGQIAKSSSPRQKLA"
     gene            complement(10692..12047)
                     /locus_tag="VFQ61_03510"
     CDS             complement(10692..12047)
                     /locus_tag="VFQ61_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9953542.1"
                     /translation="MPHSRSSVPSKSASASTEIMRTLAVRFGFCSLVSWFAGIACSAP
                     NESNGAPPSSAGSGGSQAGAASSAMGGQSSSGGTAPGFGGVQGQAGSSGGSTLMGSSG
                     AASGQGGSGGMGPPSSGGTISSGGVNSNGGANSQSGAGGSIPSAGRSGVGGTASGGRG
                     SGGAANGGNAGASSGGANSGGKSSTAGGTAGSGGSTNTGPLTIWIAGDSTVQNCSSSC
                     PCGWGSQIQALFSKDVTITNSAIGGRSVRSWLYSKTLSTADANGECDVQKDSSGNPQW
                     SDAWTRMTSTNGIKRGDYLFIQFGINDGARTCDTQGAEGRHVGIEAFKSSYAMMAEAA
                     HSRGASAIFVTPVSAISCSGSTAQGTRGAYVTATIDAGRENGVPVIDLHAASIALYNR
                     LKFCPIPGGDNEASFGTGEIGAFFCKDHTHFEAQGAAQIAGLVTQGLRDLNHPLAAYL
                     K"
     gene            complement(12149..13510)
                     /locus_tag="VFQ61_03515"
     CDS             complement(12149..13510)
                     /locus_tag="VFQ61_03515"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953543.1"
                     /translation="MRTWCSKKVTWGMCLLAAVLHAAPAAADPVPFPRAPIANRATFS
                     GRNVRALLVHDGTLYVGGSFRVTQNGVTRNNLAAFDLNGNLRPEFAPNPNGIVRALAT
                     DGNYLFVGGEFTRLALKGRLAAVDLHTGAVKRDFTAHIAGVLDVEARTGVRALAVVND
                     TTVDPPLQRLIVGGNFSRVNDVVDNRSGLCALDIHTGYLEPQLFNQGVEGGPVDALHA
                     TTDTVFVGGAFTKIQNRSASLAALNFKGVLQSGSFSTSSQPVLALDSDPAGGRLFAAV
                     GGSGNRAFAFRMTGSRRGSQLWQGPRYDGASHNVGGDVQTVHYFGGNLYFGFHDGMFV
                     EPDIYKMGALDAETGQLEVDRANQTAVCSGVEGQRAPCWLPELDSTTGAQGFFGLWTI
                     THYVDPVTNLATLIIGGEFTQVSGVSSARRLALFAEPAPEPPPPPIDPTPPPATGEQS
                     GAP"
     gene            complement(13552..15939)
                     /locus_tag="VFQ61_03520"
     CDS             complement(13552..15939)
                     /locus_tag="VFQ61_03520"
                     /inference="COORDINATES: protein motif:HMM:NF019271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EGF domain-containing protein"
                     /protein_id="HET9953544.1"
                     /translation="MSMFRLQNTAAVLLCVALWGGCGSPRDDNEILWRNLRDDTDLEI
                     TSAGSSFGGTHGEESSSAGAGGLGSQEPNHSGGAPGGGGDSAMGEGGASAAAGSGGDT
                     GGMTGESLCEPNPCLNQGTCAVEEGSVRCSCAVGFTGASCESDIDECAADHGGCDALV
                     DCVNLPGSRACGDCPPGYSGDGETGCVDIDECTSDNGGCDPLVACQNEPGSRACGVCP
                     PGYWGSGDLTNPGADGEPLGCVDVDECRGFNGGCDFRTECVNEPGSRSCTPCPPGYTG
                     TGETECVDVDECLIQNGGCDLLTSCTNEPGGRACGLCPPGFFGDGATGCFDIDECVLN
                     HGGCDPVASCVNVPGSRICGSCPAGYLGDGETGCIDVDECVTTNGGCDSLVTCENVPG
                     GRVCGACPAGYSGTGDVGCTDIDECATNNGGCDSSASCENTVGSFICHCPTGYQANGT
                     QCVDVDECATGTHDCGFGAICTNRIGSFDCACGPGFEGDGHTCSDIDECALGMVDCGA
                     NASCVNTVGSAVCPCNPGLFGSGANCQPLPALAAGNYHTCARMPDGMVDCWGANWAGQ
                     SSPPGETFIQVTSGVDFSCGLRPDRSVMCWGENWARQGIPPATPFVMIAAAGDNVCGI
                     RLDLTIACWGSNEVGQSTPPAGTFNQIAGGFAHLCALSTNGSVVCWGDNSSRQASAPS
                     GTNFIRVAAGTDHSCAVRSTGAITCWGLNSDGQTTAPRSNTGFRTVTGGGMHSCGLRS
                     TGVAACWGSNAQGQTAVPSGALFAEISAGYQHTCGLTQAGSFVCFGDNENGQSAP"
     gene            16454..19684
                     /locus_tag="VFQ61_03525"
     CDS             16454..19684
                     /locus_tag="VFQ61_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HET9953545.1"
                     /translation="MSFNDRSPRESSFSANRDSAVPTLEAREAGEVFRYWGTLLKYQD
                     ALARRPRARKPTPADSTRTLDLLQPEPGQEYTKLPFAGAETFLVDQGPLGELAMDAER
                     SLFFEDWLHARYRRSEEEESPVSELVMFPVVHLARGELSGLLRFPARIEWLTERGVFR
                     PLTYEERSPKRVANLTVSPPVRLRVHSGDRDPEQTLPFFLDVSLLSRTLRVESEAIEA
                     FFAELRRQGALTPRAMLKQVCDFLERTETGSGARAREPDVGKPPSGDSAVDCLERLYR
                     AVARRCAGAGRNRCYPVAILVNNQRSRATFHVQRDLAEASERLTNGELPAESPLTIYM
                     TGRSPGLRAQPCYGRWNRAPLTDSQRTALELALGSPFCAVQGPPGTGKTTLILNAVVE
                     QWVQSLHELARSGRPAERFLLVTSTNNRAVDNVVEPLSSGALADCPLALRLGSREVTA
                     TQTRATLERVLAFVERQANEAALDVEQARERFRSCYTEIERKFAPQRLVQQQEGEVGA
                     IDRELADLSKAARASANALASALSKLTAPSVSPTSENQASDNPRVLDAASLHVEVQNT
                     LRALGRLSRDTESDLPEIRARFETGYRRLHNKRLKALSQCFERRIDLGLPSPEAVRAL
                     TSEACETLLENAVSKLLDLESALRSEQQDQERTERRAEIEQRSKRARAELAELKVRAA
                     DPPEPSELAPLSAALFDAALALRLSWVQRNRKELAESLRAAITACERQRSLRVLLDSR
                     SGAGSHLRALFPAFGCTLLSIGNAFGQETSTFDRVIIDEAGQCHAAYAVSALLRARSA
                     IVLGDTHQLEPVVGLNREDERRISRSLKLRIAEAKLQPFRMYDESGNSAQSLAERCTT
                     QEGISERPTLRDHFRCQPEIAALCEAWCQYGMISRTPRSSKSSFAPELRAPVLFSAVA
                     GQQERSMGSYVNRAELEVVTRWVEYLFRAGVPAAELGVLTPYRGQLIALLDAFRARGI
                     PVERPDEEPDENLNLFGTQPDGGVAVGTVHRFQGGERSIIIFSTAITEPASLGFLDAR
                     VHLLNVAASRAREHLLVFGHAATLAAGRHTRELVRAGQPLPRLPYD"
     gene            complement(19681..20445)
                     /locus_tag="VFQ61_03530"
     CDS             complement(19681..20445)
                     /locus_tag="VFQ61_03530"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HET9953546.1"
                     /translation="MTQGSRFIKGARTLGSGFRILLSRPALWPWAAVPSLVFLALEAA
                     CIWGAIQRAAPWIEARVLSGEVFGHPIANRVAATGLSAIAVLLSAVLGWFLAALLTPM
                     LSAPALERLVHGTTVSLGRAAPPPLGFWREFACGLRAWLFATLLVLPLMLLLTLLGMV
                     VPGAAVVTVPLKILVSAFGLAWGLLDYPLTLRGMGARERSRRMRQHVPEVLGFATAFS
                     LLFWLPCCGIVLLPVAVMASTVLVSDWESDAKIAVR"
     gene            complement(20513..22375)
                     /locus_tag="VFQ61_03535"
     CDS             complement(20513..22375)
                     /locus_tag="VFQ61_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953547.1"
                     /translation="MDSQRWIAVLRGTSQLAVIDESGRELSRRAGPRGAVGCAVTREG
                     QLLVIGEQADSLWVFRTHVVERASSTHERNSLTIELGSPTTVELPGALRPRALAVEGR
                     TAFVADADAHLLFELDLASTTPRIVQRRETCREPIALETTSRHLVINCLADHRLVFLG
                     LSAHGRVRSPLTSIEHDGPFWALSLLPLNDELWVAASGVEDHPLQRFEGAFGYVDSFV
                     YVYRVVGRAAQRIHALNVSESHVLVPKALRWIARGSVHELWVTGYGSVNWLLYSFSNL
                     LDPPNSKGFEGVPGLVALEPLSAGAWLGASSLLDAWVRADTEPVFRVRVQPVAGASEQ
                     TPSARLGEALFFTELMAPRADSTGHRSRFTCETCHFEAGTDGRVHHTGRGEVRVSTRP
                     LLGLLENAPHFSRALDRDLTQVAHNEFRVASLGTPQNPWFSTSVERVPWLTWLGALPA
                     DLSPELLRQGLLEFLAGLEPAPNSHVANALAHGRSSFTSIEAAGAEVFARRCVGCHAA
                     RLFSDEPDSEVPFGTWSQWIFRDDGPVVWARGDYEKTAVLPYVHELGTRIPSLRRVDR
                     KYPYFTNGSAHSLQSLLESFAWTRERSFHANAPAEAARLDPGERTALLAFLRLL"
     gene            22951..23358
                     /locus_tag="VFQ61_03540"
     CDS             22951..23358
                     /locus_tag="VFQ61_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9953548.1"
                     /translation="MRNVPRVQQYMTPSPITIGRNQPMSVAHRLMREHEIRHLPVLEA
                     GRVLGIVSAGDLHLVETLRDVDPNRVTVEEAMTENPYTVPPSEPLDRVVTEMAEHKYG
                     CALVVDHHKVVGIFTTVDACKAFADHLDGRNPT"
     gene            complement(23382..24146)
                     /locus_tag="VFQ61_03545"
     CDS             complement(23382..24146)
                     /locus_tag="VFQ61_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953549.1"
                     /translation="MPLDEFARDFAASLPESYRRNFDSAAIRCHAQLALARKASPAIV
                     CTFESNRVPGSALCVVADDRPGLLATISAALVMCGLDVIEAEAYTRRIERQHDEAVDV
                     FWVRHESLEQRHAPVTQEEVERLRQTLVGLLEGRLERRRFVDGQRVPPTPASRETVVR
                     FIENADGDFSTLEVETGDRSGLLLALSQALYNQRVQIIGSQVKTVNNRVYDRFHIVEL
                     DGSAIGPSRRLEIQVAVLTAVDPVLERPRASASVRS"
     gene            complement(24272..25981)
                     /locus_tag="VFQ61_03550"
     CDS             complement(24272..25981)
                     /locus_tag="VFQ61_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9953550.1"
                     /translation="MSLTVGDLVDGKYRIVRLIGDGGMGSVYEARHELLGIPVALKFL
                     HEGLAKRPGLSARFLQEARVSATIQSPHVARVTDVDTTPDGAPYLVMELLSGESLQAL
                     LTRVGRIGRDQAVDYGLQILVGLEAAHELSVVHRDLKPDNVFVTPSPGGPVLKLIDFG
                     IAKLRVAAEYRKELTRAGVVMGTPEYMAPEQFYAAHEVDARADLYSLGVMLFEMLTGA
                     RPADGDTPEVIVGNVIAGHVKHIAELAPDLPESLVSLVNRALEPDRDRRFASAFAMRS
                     ELSRCAGELSAAGRAAATAARVMREERSVVLGTGTSLAKPQVPDTLDNDRPNKLPDTL
                     PPDGEGEMPRRTGTQAAPPPHVWGGGASPAAPVAAQAPAPIPPAGVVPPPMRAATQRR
                     RRSNNRALIWLGLSAVALIAGIGFVLSQSTLGSRRTVPDLPAFDVPATGQPATTPSVA
                     VVPTTPATNPDNVVPERPPAGSPNPTSVRPRPPSTPAVGGPVSTSTSSTPSTPPFGTN
                     PLPFPSIALPSSLPPLPSGFPTALPTVFPTALPTFLPQIPGLTVPGLTPAPGTTTPPP
                     PAP"
     gene            complement(25978..26550)
                     /gene="def"
                     /locus_tag="VFQ61_03555"
     CDS             complement(25978..26550)
                     /gene="def"
                     /locus_tag="VFQ61_03555"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET9953551.1"
                     /translation="MAIRKIAQLGEPVLRQRAREVSREELASPRMQTLIDDMVETMRD
                     ADGAGLAAPQVYESVAIVVIEVRNNPRYPQAETIPLMALVNPQVTPLVDADQPENAVF
                     LYEGCLSVNGIRGRVRRPRRVRVRGWDRAGAELDFEWEGFRAVVVQHETDHLNGTLFV
                     DRADPRTLTFSREYDRYVPLDARFVDGVRP"
     gene            complement(26596..27168)
                     /locus_tag="VFQ61_03560"
     CDS             complement(26596..27168)
                     /locus_tag="VFQ61_03560"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET9953552.1"
                     /translation="MRIYTKTGDDGSTGLFGGARVDKDSARVEAYGTVDELNAAIGVI
                     RAERSGQPSALPDEIDAMLATFQNTLFALGAELACVPGKEDKLRMRLLDDREISALEA
                     AIDGAEAELPPLTSFILPTGSRVAALLHQARTICRRAERRVLSAGRVAPVRGEVLVYL
                     NRLGDLLFVLARRANQLAGRPDVPWLAAQS"
     gene            complement(27239..>27867)
                     /locus_tag="VFQ61_03565"
     CDS             complement(27239..>27867)
                     /locus_tag="VFQ61_03565"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9953553.1"
                     /translation="IRFAVAADDAEHFASDPSLKLVVANSELMSDARAVVTTARGRGS
                     SASFVVCAAPREISVLRSALTGLTSVTVTDGFASPENVLFVANELTSGRSSGRTSPRI
                     PYGLSVAFRSAGREVDEHGYSYNLSERGLYVRTLALPDEDEVWLELTPPRLDRLVRLV
                     GKVAWRRPFNNLGSATVPPGFGIEIIDGSARDRALWVESYNELSRALG"
BASE COUNT         5238 a         8949 c         8609 g         5071 t
ORIGIN      
        1 gtaaccatac cttttcagcc agtttcaggt gcacgcgggg cccgaccgca aaggtggtcg
       61 tgtctcgagt ggcgggcgct gccgaaactg ctttcggtgt ggacagccaa cgctggtgac
      121 gcagctcgac acccagcgaa agctcaggga tcaagaagta gcctacgtga acgccagagg
      181 taaagttcgt acgcgagcta tcgggctgtt tgtcttcccc gcgcacgcgc gtgagctgca
      241 gtaaggtggc ctcgatttga gccgtgaatc cgccctggat gaaggccaag tcgacgccag
      301 ggaacagcac tagatcgttc accgcaaaca gcgcgttatc catcgccgag cgcgctctga
      361 tgccagcggc attcgccgcg gcccgctgtg cgttgggagt gtttccgccg ccgttaccga
      421 gcggcaacgc gacgccgagc gtcgccgcga agcgcagcgc ggaccccaat ttcacgccgt
      481 acaagccacc caaaattgga tttacgaaac tggaacccgg ctccccgctg ggcggtgagt
      541 tcgaaacggc ggcgaaacgg gcgaacgccg cgaactcgtc gctgagtttc atactcgcca
      601 gtagcgtgct cgcgatcgtc gagcccgtcc ggccattggt tccctcgtaa tacgccagcg
      661 tcgtgtccga acgaaccaca ttggcgggaa tcacggaacg caattgaaat ggcagtgagt
      721 acggtggcgg tttgggtttg ctggcaggtt gcggcaatac cggcggcgac tcgaaactga
      781 gagttcccgc aaccgtcggc gtcgccgtgg actcaacagg agtaggtggc tgcggactgg
      841 gtggctgcag actggatggc tgcggactgg gtggctgcgg actgggtggc tgcggactgg
      901 gctccgcggc agcagggtcc acgggaatgg ttggcgtttg agcaaaggcc aaggagagac
      961 ggaacgtcat cgacgccgcc gcaaatgcaa gcacacgtat cttcatcttc ggaatctcgg
     1021 gaaggcgaca cacaccggaa aagcagccct gcacgagcct gaaacggccc atgaagctgg
     1081 cacggacgag caccgctgcg aatccacacg accgttccgt cgtggtcttc gaatcagcgg
     1141 gtagctcggt agctagctcg gcaaacagct agagcaacac gcatgccgtt cgtcataccc
     1201 tgtgggtgac agatggaatc agcgctcgat cctgagcagc gaaacggctg cgaaatgccg
     1261 cggcattcgg gagttgcaac agttctgtcg ttcacgcgat ctcggaacga cagccttgtc
     1321 gttccgagag ttccggcact ctcgcgaaac aaagtctgcc gtcacgcgac gcactcgtga
     1381 tgcacggcgc cgtggccgaa acggcttaag cctctaggag gggctgcact tgttctcgga
     1441 tccagtcgcg aaaacgggcg acacacgggc gccgtaaatt ctcacgtggc cccaccaagt
     1501 aataggcaaa gccgctcggg agcggctcgg cctctggaaa cggcaacacg agatgccccg
     1561 cggcaagctc atccgcggcc aaagagaagc gcgcgagggc catgccctgg ccgcgaatcg
     1621 cggcttccac caacatgctg gagtccgaga tgcggtggca gcgggcgcgc gatagatcgc
     1681 ggagcccagc ccgcgttagc cagtgttcga gtgcggtcgg atgatcatcg gtgatcagcg
     1741 tttctcgcaa caagtcgttg gctagccaac gccggcgctt tcgatgcagc gcggggttgc
     1801 acaccaacac gaagtgatcg gcatatagct tctccgaaac caagcccgtg taccccccgg
     1861 agccgtagcg gatgccgaga tcgaacggct cgcgggagaa gtcgaccagg gcttcggtcg
     1921 cggaaatgcg cacgtcgatg tcgggatata gggccatgaa tttgccgagt cgcggcacga
     1981 gccagcgagc acagaaagac ggaatgctgc tgatcgcgac catctgtcgc tcataggcgg
     2041 cgacccggcg cagcttgtca ttggcatcgt agagacgctg aaacacgacg ctcaactccg
     2101 ccgcccacgc ctggccctcg cgcgtgagct cgagccggcc tggcaagcga cggaacaaag
     2161 atacgccgag ctcggcttcg agactcttga tttgatggct caccgcggtt tgcgtgacct
     2221 tcaactcctt ggcggcccgc gtgaagctgc cgtggcgtgc cgcggactcg aaggccaaca
     2281 acgcattcaa agaaggtagc ggagcacgcg agtcgcccat gagaaaaact catgtagcat
     2341 gaaaaagcct cgctcgcgcg acgcggtccg aaccgctaca gatgggctca tgaagctctt
     2401 tcgtttgcgc tacagcccgt atgcccgcaa ggtccagatg ctgctcgatt tgatcccacg
     2461 tcgctacgag tccatcgaag tcagttacgg agaccgcaac gaactcgccg agctcaccag
     2521 cggatatatc tacgtacccg tactggagga tggtgggaaa gtgatcgtgg actcgcgacg
     2581 catcgctcag cacctgctgt ccgacccgaa cgcggcgcac ctcgtccccg cgcctcatca
     2641 agggccgatc tgggcctatg cagatttcgc ggatggaccg ctcgaagacg tgctgttccg
     2701 gatcgcctcg ccgttcattc gcgatcagtg gcaaacgccc ttcgagcgtg cactgtatac
     2761 gctgatcaaa gagcgcaagt tcggcgccgg ctgcctggat gcgtgggccg cgcagcgtga
     2821 cgagctgttc gcgcgcgggc gggagcttct ggagcccacg ttgctcacct tgggtcagca
     2881 gccgttcttg ttcggccaga gcccaacctt ggcggacgcc gcactatacg ggctctgtga
     2941 gatgctagag gaagccaagc cggagctgct ggcgcagttg tccccacggc tcccggagta
     3001 cgcccggcgg ctcgaacgct tcgcgaaaga gcgcgttcgc tgaagtgatt ccgtgctcga
     3061 cacgcgcgag cacattggcc ctcttcgcag ctggttctag gcgcgcgcct gttttagagt
     3121 gcgcgccatg ctcgtctcgc gtcgcactct tctcgcctcg acaatcgtct cgggaggcgc
     3181 ctggattggc gccggagcgc tgctgttcac cggttccggt tgccgcaaga agcccgcccc
     3241 gcctgggccg gagcgcctgt acgtgagcac ggaagaaggc ggcgaggtag tcgtgctcga
     3301 ggcgacgact ttggcgatcg tcgcacggat cccggtcggc aagcgcccgc gaggtttgaa
     3361 attggccagc accgggagcg agctttatgt cgcgctctcg ggctcgccgc gcagtgggcc
     3421 gggcgcggac gagagcaagc tgccaccagc cgatcggcgg gcggatggga tcggggtggt
     3481 ggacttgacc gtccgtaaac tcatccgcac gctcgagagc gggcaggacc cggagacgtt
     3541 cgacctgtcc aaagacgggc aacgcgcctt catttccaat gaagagacgg ccgagttgag
     3601 cgtcttggac ttgaagtcgg gccacgtcgt ttcgcgagtt gcggtgggcg atgagccgga
     3661 aggcgtgtcg ctcagacccg acggggccgt ggtgtacgtc acgtcagaag ccgacggcgg
     3721 agtatccgtg gtcgacacca aaaccttgaa agtcgtagcg cagttcacga ccggcccgcg
     3781 tccccgggtg atcgcattca ccaagaacgg taagcaggcc ttcatcagca acgagctcgg
     3841 tggcagcgta agcgtcgtcg atgccgtggc tcaccgagtg acgggagaaa ttcccgtcac
     3901 gggcccgggc cccgcacctc accgcccgat gggattggtg ctctcgagcg acgaagggca
     3961 attgttcgta tccactggac gcggcggagc cgtagccgtg atcgacgtcc tcgcacgcaa
     4021 agtgagccgc tggatcagcg gcgtcggagc gcgcccatgg ggcctaaccg tgagccgcga
     4081 tggcaagcga ctctacacgg cgaacggtcc ctccaacgat gtatccatgc tcgaggtcgc
     4141 caccggtcag gtgctgaagc gcgcggagat cggcggtttg ccgtggggca tcatcgccgg
     4201 ctgagcttcg cgccaatttc gatacccgtg gcagcgtgta aggtcgctgc cacggggaat
     4261 ggtccgcgtc gtcgctccgc acatgcgcga tgaacgaccg ctggcgcgcg gcttacgggc
     4321 tcttgctcgc ctcctcggtt ccaccttcgg caccgaggtt gatgcgacca ccgttcggcg
     4381 cgggctcgtc tccgacgtcc tgatcgggat cgccccggtc gatcgcaggg ctgctctcct
     4441 tgtctttcac ccaggactcg ctcgatgcat cccagcgccc ggcttcggat ttgaggtgga
     4501 aatcgagatt tgcggggtcg acgaagcccg ggtcatcgca gccggtgccg acggtcaaga
     4561 tgccttccat gtccgccaac agggcaccgg tgttatcgat cacgtcctcg ccctcttccg
     4621 tcttcaagct gccgatgcag ttgtatttga agaccgtgtc cccggcttcg tacatgacgt
     4681 cctgaagcgc tgagccccag atgatgttgt ttacgaccgt tcccttggca ttcccgtagt
     4741 cgtccacgtg gtgatagagc cgaatgccgt attggttgcc gtagatcgtg ttgttgcgca
     4801 ccatcggatc ggcgtcgtcc ttgatgccga tcccgaggaa gttgttcacg attaggttgt
     4861 tctcgagcgt cggagtggcg ccatcaccat tcgagatgcc tttgtcctgc ccgcccataa
     4921 tcagattgtg gtgcacgaac gccgacgttc cctcgcccaa atcgaggttg tcgttgatct
     4981 ggttgtcgaa ggtgcagaag cggatctccg gtttgcccac gccgtcggag tcgatgccgt
     5041 cgctggcgtt gcctttgaat agagaatatt cgaccaaggt atcggtggct ttgaggttga
     5101 gcccatcgtc accttcgttg ttgatgaatt ggcagtgcga aatgcgcgcc tttgcgttct
     5161 tcaacgacaa cgcgccgcgc atacctacgc cctcgaagtc ggactcatag ccgtgctcga
     5221 aggtgacgta ctcgaatacg ttgttgtcgg agcccttggt aaagttggtg agagatcccc
     5281 aatgcttatc cttctccaac caggtgaaga ggatcgggct cgactcggta ccgcgcgcgt
     5341 ccacgtcgtt ctgcgcgtga atgctgacac ccggcccggc cgcgatcgtg gtcccaggca
     5401 tgagcttcca ggtggcggtc tttggcagca ccgcctcttc cgtcacgctg tgcttgcccg
     5461 ggtgcatgac gatcgagccg gtccagtcgt catcctgcgc gatcgcgtcg agcaccgcct
     5521 tcaatgaggc gaaaggttga gcttcggtgc cggtcccacc cgccaccgcg cccaccgaga
     5581 catgtagttc cttgccgagg ttcaacttgg ggggcttcat ctcaggcggg ttcggtgggg
     5641 ccgaaacctc cgggtcatcg ctaccgcagg ccactgagag ataggcggcg cttgcaagca
     5701 agcacgcacg accgagattt cgcaggatgt tttcagttcg cattggactc aactcgatgc
     5761 tcctgaactc gacacggttc tcgaaaggaa agcgctcaat gggccgagct cgaacccacg
     5821 gttcgcagct cggctggtac caggcagata ctcgtcgacg ttactcgaag cagacgcatc
     5881 tgcgacaaag ggcttcgaac ggttggtgtc ggatgcgcct cattccgagg ctgtcatttc
     5941 acaattcttg gcgatttgga ggcgtgcatc gcccgcgtgt ttgaccatgt actcgcgcat
     6001 acgggtcacc tcggactgcc acgccgaaaa ctcggtgacc gtgtcctcgg ccattgatcc
     6061 ttcgatgacc gaggccatct catcgacctt ggtgggatca tagacctcgt ctaggtactc
     6121 gttcaaaagc tcgcagtagc gcgccttgaa cacgctggtc aggcgcgtga gcagcggctg
     6181 ccgcttgcct cgcggcccga accagcccgc cactgacgtg aaaggatcac agctctgagg
     6241 atagcaggta gcgcgctgca acacggtatc gtagtcccag ccccaaacca gcatgcgccc
     6301 gccgtgcgga gcggcggcgt ccggtttgaa atacaggaag tagttgcggc gcgcaccgac
     6361 gaagtcttcg agcgagggca ttattgactc ggcagcaatc tcgcgcagcc actgttcgac
     6421 gtcgatccat tcactgatgc aggttttgaa gtcctcatcg tcgggcgtct gcgccggatc
     6481 gccgcacttg agcatcggga tcagattcac ctccgcgtcc tgcggcgtac cgcgcaggac
     6541 ttcgtagatc ttcgtgtagt cagggacatc gaaggtgtca cccttgtagc cgaggtcggc
     6601 cagagaatct ccgtagccgc aagacgcaga agccgcgtac aggaagccgt tgctcgtctc
     6661 gaaatggttc ttcaaaaagc gggcatcctt ggcgcgctcc acgctctcca tcacgccgta
     6721 aagcttgcca ttgatgaaca cgcgcaaatg gttgcagagc ggcgcctcga gcccgaagtc
     6781 ccgcatgagt ttgtaaccca tgcactggag catgttcgag tcatcgcctt cgttcgagag
     6841 cgcgttgacg cggctcagcc cggcaaagcg gcggttgttg tccttgggca gcgacttggt
     6901 gaagtcgagt ttgaagccct gcaaccgatt cacgccgcgg ctcttggtac cgcgcagacg
     6961 catggccacc ttttccaacg tcgtgttgcc gttcccgtcg gggctttcgt aggtcatcgt
     7021 gacgggcacc caatcgtaag gtccgcagtg gttgttccat ttgtcccaca gcagatccaa
     7081 ccactggccg ggctcgtagc cgtacgactt gagggtggcg tcgtcgatcg tgagcttcaa
     7141 atccgcgacc tgctcgttcg agaagaagtc acgcgtggat gggccacagt cacccatgca
     7201 tccgccaatc acctcgctcg ggggattcgg gctttcggtt tgtggctggc ccgacgcggg
     7261 ttccccgcct ccacaagcga gcatcaaccc gctcaatgtg gtcatgaaca acgtcgatat
     7321 cgtggacaca cggtgcagct tcaaggttcg cctcttggag atgtcgtcga tcttcgcggt
     7381 gacgcggggc acggcagcgc gaggctaact atgaaagtct cgcggccgtc ccgcaatatc
     7441 acagcgaaca ctgcgtgacg aacccgtgtc ccttgtaacc gagcacgaat gttccgactt
     7501 tgacggaggc tgcgaagatc ccaaaaaaga tctgaggctc gcagcaagcg tggtgaggct
     7561 cgttcgcctc agccaatctt cgacgtgggc cacgccggag ccaaacgagg cagatttggg
     7621 tcactttttt tttcgaaaac tcgcgagcat gagctcagct ctacgcagca tgcacgcgag
     7681 gtgcttcgaa tcctcgagaa ataagggcga gtgcaatcgg gttgcgtcga cgcgatcgac
     7741 agcgtttctt ccccgccttt gttcgacgtg gcaacgctct caccggcgcc tctcccttac
     7801 ttcagcgcgt gtgctgcgcg cgtacgctcg tagctcgagt tcgcttgctc gccgggagcg
     7861 ccgatggctc ttctggattg agcagattgc ttttgctcgg acggctcgga ctagcccgcc
     7921 tttaccgcag tgtctccctg gtagtagccg ttcccactat ctagcaggca acatttttgg
     7981 ggagcgtcag agggccgggc ttctcccaat tgtcgcgctg gatggccgaa ctagcagcat
     8041 cggtgtggaa ctgaaaccga agtccacctc cgaaggaggc acgaatccca gcttgcgata
     8101 aaagggttgt gcatcggcgg tcccgagccg cacgacccgc gaagcgcgca ctttcggatg
     8161 ttcgaggaga aactcgatca agcgtcggcc gatgccacgc ccgcgctgac gttcctcgat
     8221 caccacgtcg gccaagtagg tgtgcttcga gttgtcgctg accgcgcgcg cggttcccac
     8281 caactcaccg gacgcgctgc gtactccgac ccacgcgctc gactcgaggt gcgcgcgtcg
     8341 cagctcctgc tgtgagtagt ctcgattcca atattggtct cgaatcagcg cgaacgtcgc
     8401 cgcccagtct tgctcggtca actcgggttc gagccgcagc ccatgtgacg cgatcatgaa
     8461 ccgtgcggga cgttccaacc cgggatttcc gccaaagatg cgctgaatcg cgtgcacgtc
     8521 gcgaactcca cctctttgcc acaagccttc gatcacttgg cggatcactt cgggcggctt
     8581 gttttgcaaa accttcagtt tgcccgcgat ccgatcgagg cgaacaccga acacgagcac
     8641 cccgcggacg gcccctcgat acatctcgga ggtgtgatcg atcggcgcgt accccccctc
     8701 cggctgatac ttctgcatca atgcggtcag catgcgagct ttcagttcgc cgtcttggat
     8761 gctctcgagg gtcccttgcg ccatggcgct ctcgtagaga ctcgttgcgg ggcaggctcg
     8821 ctctggatcc gtaaagtagc tcggcacggt cgcgagcagc tcgtcggcgc tcaccaccgc
     8881 agttcggccc aggcagcgcg ccttctcacc ggccttggcg ccgtgaaaca acagccaatc
     8941 gtcgagtacc acggcgttca acgtgcgcag caccggctcg ccttcgggag tactggtggc
     9001 caaacgaatc aactcggccc gcgccaggaa ctcgcgtgcc tgtgcgcggt ccatgctgaa
     9061 ctcagctcgt ctcatgcttc aatcgtgccc gggcactggt ctgttttgca gatccgattt
     9121 ggaaatatta cacagaccag atggagtacg tcccaaacct cctggatccc cacgagcccg
     9181 aagcaaccgc ctcggcagcg ccgctgttcg cgaggatcgc ccaagctatc gccgaagaca
     9241 tacgccgggg tcggcttcgg cctggccggc ggctgcccgg gtcgcgtcag ctcgcgctga
     9301 gcttgggagt tcaccgcaac accgcgctcg ccgcttacac cgagctcgag cgccagggct
     9361 acatcgtgac tcgccgcgcg cgcggtactt tcgtggcgga agcgctgccc gaggtacgtc
     9421 cgcgcaagct gcgtccgagc gcggcgcgtc atcacgactc ggtgctctcg ctaccgccct
     9481 ggcccaaagc gctgataccg cgtttgcctg agcagccccg gttctcggcc aagagcttac
     9541 ccctcttggc cggcatgccc gatccgcgct tgatgcccag aggtgcgctg tcgcgcgctt
     9601 atcgccgggt gctcgcgcgt gagcccgacg tcctgaacta cgcgagccca caagggcatc
     9661 cacgactcgt tcgcgcgctc tgcgagcaac tgcgtactac gcgcggggtg ttggcctcgg
     9721 agaacgaaat cgtgatcacg aggggcacgc agatggcggc cttcatggcc gcgctcgcgt
     9781 tgtgctcgcc cggcgatcgg atcgcggtgg aggctctcgg ctacccgccc gtctggcaag
     9841 cgtttcgtct agccggtgcg gagttggtcg cggtgccggt ggatggcgcg ggcatgcgtg
     9901 tcgacaaact cgaggcgctg cttcaaacga gcaagattcg cgcggtttat gtcacgccgc
     9961 atcaccaata cccgaccacc gtgagcatga cggcggcgcg tcgtttacag ttgctggcgc
    10021 tcgcggagcg ccacggcttc gcgattctgg aggacgacta tgatcacgag ttccattggt
    10081 cggggcgccc actgctgccg atcgcgagcg tagcgaacgc caagtgcgtg gtgtatctgg
    10141 gcacgctcgc caaggttttg gccccgggtc tacgcatcgg gtacatggtc gcgcccgagc
    10201 acgtcgtcga gcgcatcctg gctctgcggc gcttcatcga ccgacaaggt gaccacgccg
    10261 tggaagcggc ggtggccgaa ctgattgaag atggcgagtt cgaccggcat gccaagcgaa
    10321 ctcggcgtgc gtacctggag cgacgcgaga cgctgattga aggcctgaag cgtgagttcg
    10381 gtgaacgctt gcattacgac ttgccgcacg gcggcttggg cctatggctc cgcgctgatt
    10441 taccgatggg agcgcagcgc ttcacggagc tggccgccga gcagcaggtt cacctgcagc
    10501 acggaggtca attctcgttc gatgggcgcg cgtcggaatt tctgcgggtc ggcttcccgc
    10561 gcttgaacca gaccgagatc gagcgggcgc tgcgcatact cggccagatc gccaagtcat
    10621 cgagccctcg ccagaagctg gcgtgacagg ctcactttcc tggaaccggc ggagcgcgcg
    10681 gttccgggtc gttacttgag gtacgcggcc aacggatggt ttaggtcacg taagccctgc
    10741 gtgacgagtc cggcaatctg cgccgcccct tgagcctcga aatgagtgtg atccttgcaa
    10801 aagaacgcgc caatctcgcc agttccgaat gatgcttcgt tgtccccacc tgggatcggg
    10861 cagaacttga gacgattgta gagagcgatg ctggcggcat gcaggtcgat caccggcact
    10921 ccattctccc gaccagcatc gatcgtggcc gtgacgtaag cgcctcgtgt gccttgggcg
    10981 gtgctcccgg agcaggaaat cgcactcacg ggtgtcacga agatggccga tgctccccgg
    11041 ctatgtgccg cttccgccat catggcgtaa gaggatttga aagcttcgat tccgacgtga
    11101 cgtccctccg cgccctgcgt gtcgcacgtt cgcgcgccgt cgttgatacc gaattgaatg
    11161 aacaggtagt caccgcgctt gatgccgttc gtggaagtca tgcgcgtcca ggcgtcactc
    11221 cactgagggt taccgctgga gtctttttgt acgtcgcact cgccattggc gtccgccgtg
    11281 gagagtgtct tcgagtacag ccagctgcgc acactgcgcc ctccgatggc gctgttggtg
    11341 atggtgacgt ccttcgagaa gagcgcttga atctggctgc cccagccaca aggacaggag
    11401 ctgctgcagt tttgcacggt cgagtcaccg gcaatccaaa tcgtgagtgg gcccgtattc
    11461 gtgcttccgc cagacccagc cgtaccgcct gcggtgctcg actttccgcc cgaatttgcg
    11521 ccgcccgaac ttgcgccggc attaccaccg ttcgccgcac cacccgagcc gcgaccacca
    11581 ctggccgtgc cacccacacc gctgcgaccc gccgatggta tgcttccccc ggcaccagac
    11641 tgcgagttgg ctccgccgtt ggaattcacg cctccgctcg agatggttcc acccgagctg
    11701 ggagggccca ttccgcctga gccgccctga ccggaagcgg cgccactcga gcccatcagt
    11761 gtgctcccac ccgaagatcc ggcctgacct tgaacaccac cgaatccagg ggcggtacct
    11821 ccagagctag actgcccacc catcgccgag ctcgcggcgc cggcctgact tccgcccgag
    11881 ccagcgctgc tcggcggcgc cccgtttgac tcgttgggag cgctgcaggc aatcccggca
    11941 aaccagctca ccaatgagca aaacccaaac ctcaccgcca acgtacgcat gatctcggtc
    12001 gatgccgagg ccgacttcga gggaacagag cttcgcgaat ggggcatggt gtggatgaga
    12061 cctcgccatg gctctgttgc gcggcgcaac tcaaactggt caaatttcga ccttcgagcg
    12121 atgagactgc atcattccga tcgcgctctc acggcgctcc gctctgctcc ccagtcgctg
    12181 ggggcggcgt cggatcgatg ggagggggtg gaggctcagg cgccggctcc gcgaacaggg
    12241 ccagacggcg tgcactactc acaccactaa cttgggtgaa ctccccaccg attatcagag
    12301 tggccaggtt ggtaaccgga tcgacgtagt gcgtgatggt ccacagcccg aagaagcctt
    12361 gcgctcccgt cgtgctatcg agctcaggca gccaacaggg agcgcgttgc ccttcgaccc
    12421 cggaacacac cgcggtctga ttggcacgat cgacttcgag ctgacccgtc tcggcgtcga
    12481 gcgctcccat cttgtaaatg tcgggttcta cgaacatgcc gtcgtggaac ccgaagtata
    12541 gattccctcc gaagtagtgc accgtttgca cgtccccacc cacgttgtga gaagcgccgt
    12601 cgtagcgtgg gccttgccag agttgggagc cacgtcgact acccgtcatt cgaaatgcga
    12661 aagcacggtt tccgctgcca cccacggccg caaacaagcg acctcccgcg ggatccgagt
    12721 ccagcgccaa cacgggctga ctgctcgtgg agaagctccc gctctgcaaa acacccttga
    12781 agttcagggc cgcaaggctc gcactgcgat tctgaatctt ggtgaacgct ccccccacga
    12841 acaccgtatc ggtggtggca tgcagcgcgt cgaccgggcc gccctccacc ccttgattga
    12901 agagctgggg ttccaaatag ccggtatgga tgtcgagagc acatagccca ctccggttgt
    12961 cgaccacgtc attcacccga ctgaagttgc cgccgacgat caagcgttgg aggggcgggt
    13021 cgacagtcgt gtcgttgacg acggccaacg cgcgaactcc cgtgcgcgcc tcgacgtcca
    13081 acactcctgc gatatgcgcg gtgaaatctc gcttgaccgc ccccgtgtgt aaatcgacag
    13141 ccgcaagccg gcccttgagg gccaaacgag tgaactcccc gcctacgaat aggtaattcc
    13201 catccgtagc cagagcacgc acgatgccat ttgggtttgg cgcaaactcc ggcctcaagt
    13261 tgccgttcag atcgaatgcg gcgaggttgt tgcgcgtcac tccgttctgc gtcacccgga
    13321 acgatccgcc tacgtaaagc gtgccatcat ggaccagcaa ggctcgcacg ttgcgcccac
    13381 tgaaggtcgc tcgattcgca atcggcgctc tcgggaacgg caccgggtcc gctgctgcag
    13441 gcgccgcgtg gagtacggcg gcaagcaagc acatccccca agtcaccttc ttcgaacacc
    13501 aggtccgcat ccgatccttc ccttggccgg tctggccgcg tgtgccgaaa gtcatggcgc
    13561 gctctgcccg ttttcgttgt caccgaagca cacgaagctg cccgcttgcg tgaggccgca
    13621 cgtgtgttga tatcctgcag agatttccgc gaacagggcg ccgctcggca cggcggtttg
    13681 cccctgcgcg ttgcttcccc aacacgccgc aacgcccgtc gagcgcaagc cgcagctgtg
    13741 catcccgcct ccggtcacgg tacggaaacc ggtattgctt cgcggcgccg tagtctgtcc
    13801 gtccgagttc aagccccagc aggtgatggc tccggtactg cgcaccgcgc agctgtggtc
    13861 ggtacccgcg gctacgcgga tgaagttggt tcccgagggg gcggaggcct gccgagaaga
    13921 gttgtcgccc caacacacca cgcttccgtt ggtgctgagg gcacaaagat gggcgaatcc
    13981 gcctgcgatt tggttgaagg tgcccgccgg tggcgtgctc tgcccgactt cgttcgagcc
    14041 ccagcaggcg atcgtcaagt ctagacgaat gccgcacacg ttgtctcctg ccgccgcaat
    14101 catgacgaac ggcgtcgcgg gcggaatgcc ctgacgggcc cagttctctc cccaacacat
    14161 gacgctacgg tccggcctca aaccgcagct gaagtccact ccggaggtga cttgaatgaa
    14221 ggtctcaccc ggtggggacg attggccagc ccagttggcc ccccaacaat ccaccatgcc
    14281 gtccggcatg cgagcacacg tatggtagtt acctgccgca agcgctggca gaggctgaca
    14341 attcgcgccg ctgccgaaca gacccggatt acaggggcaa accgcgctgc ctaccgtgtt
    14401 gacgcagctg gcgttcgcgc cgcaatccac catacccagc gcgcactcgt cgatgtccga
    14461 gcaggtgtgt ccgtcacctt cgaagccggg gccgcaggcg caatcgaacg agccaatacg
    14521 attggtgcaa atcgcaccaa agccgcagtc atgagtgcct gttgcgcatt catcgacatc
    14581 gacgcactgg gtgccgttcg cctggtaccc cgtggggcag tgacagatga agctgcccac
    14641 ggtgttttcg cagctggcac tcgaatcaca accgccattg ttcgtggcgc attcatcgat
    14701 atcggtacaa ccgacgtctc cagtgcccga gtaccccgca gggcaagcac cgcacactcg
    14761 gccccccggg acgttctcgc aagttacgag gctatcacac ccgccattgg tggtaacgca
    14821 ctcgtccaca tcgatacagc ccgtttcacc atcgccgagg taaccggccg ggcaactacc
    14881 gcagatacgg ctacccggaa cattcacgca gctcgcgact ggatcgcagc ctccgtgatt
    14941 cagcacgcat tcgtcgatat cgaaacagcc ggtcgcgccg tcgccaaaga acccgggcgg
    15001 acataggcca caagctctcc cgcctggttc gttggtgcaa ctcgttagga ggtcacaacc
    15061 gccgttttga atgaggcact cgtcgacgtc cacgcactcg gtttcgcccg tgccggtgta
    15121 cccgggggga cacggcgtac agcttctact cccgggctcg ttcacgcact ctgtcctgaa
    15181 gtcacagccg ccattgaacc cgcggcactc gtcgacatct acacagccca acggctcgcc
    15241 atcggctccg ggattcgtca aatcccccga cccccaatac ccgggtggac ataccccaca
    15301 agcacggctt ccaggctcgt tctggcaagc cacgagtgga tcgcagccgc cattgtcact
    15361 ggtgcactcg tcgatatcta cacagccggt ctcgccgtca cccgagtatc caggggggca
    15421 atccccgcaa gcacggcttc cgggcaagtt cacacaatcg accagagcgt cgcagccacc
    15481 atggtccgca gcgcactcat cgatgtcgct ctcgcagctg gcgcccgtga acccgaccgc
    15541 gcacgagcag cgtaccgagc cttcttcgac cgcgcacgtg ccttggttca ggcaaggatt
    15601 cggctcgcaa aggctctcgc cagtcatgcc tccggtatca cccccgcttc cggccgccgc
    15661 gctggctccg ccctcgccca tggcgctgtc cccgccgcca ccgggagcgc cgccgctgtg
    15721 gttaggttct tgcgaaccaa gcccgcccgc gcctgcactc gagctctcct ctccgtgagt
    15781 tcccccgaag ctcgatccag ctgaagtgat ttccagatcg gtatcgtctc ggaggttacg
    15841 ccacagaatc tcgttgtcat cccgcgggct accgcagcca ccccagagcg ccacgcagag
    15901 cagcacagcc gccgtattct gcagcctgaa catcgacatt ctttccctcc gccaatctct
    15961 cttcacggag caatcgggcc cggagcacac cgcacgactg aaaagtaacg ggctcagttt
    16021 ctagccaagc catgctcgtt tgatcggctc gttctcggtc gctcgttttc gagcgctcga
    16081 ggcctacgcc ggcatggttg cttcgagcga gccttggttc ggtgcagcca cccgcacgag
    16141 cggcacaacc gacttcttcc actatatgaa attcacttgg aaggtcgcaa tcgaaaacag
    16201 cgaacacttc gcggctcacg ctcggtcaag cgatgacgcg agcggctcgc gatcttactc
    16261 ggatggcaag ggcacgtaag gcgcccgagc agctcgtatc gctttcgctc gtctgaggct
    16321 ctgcgcggcg ctcagactct gctcgattct atggaggccc cgaccggaat cggggtgttt
    16381 gccagcattc ttgagccagt ttcccgcgaa cgtggcaaag accggcagtt ttaatcatgt
    16441 tggtccgccc tggatgagct tcaacgaccg ttccccgcgt gagtcttcct tttcggccaa
    16501 ccgagattcc gcggtgccta ccttggaggc gcgcgaggcg ggagaggtgt tccgctattg
    16561 gggcactcta ctcaagtacc aggatgcgtt ggctcgccgt cctcgagctc gaaaacccac
    16621 cccagcggat tcaacgcgaa cactcgatct cctccagccc gagccaggcc aggagtacac
    16681 gaagttgccg ttcgctggcg cggagacctt cctggtggat caagggccac tcggtgagct
    16741 cgcaatggat gccgagcgaa gcctcttctt cgaagattgg ttgcatgccc ggtaccgtcg
    16801 ttccgaggaa gaagagtcac cggtgtctga actcgtgatg ttcccggtcg tgcacctggc
    16861 ccgcggggaa ctctcgggtt tgctgcgctt tcccgcacga atcgagtggc tcaccgagcg
    16921 cggcgtgttc cggcctctga cctacgaaga acggtcgccg aagcgtgtgg cgaatctgac
    16981 cgtctctcca cccgttcgcc tgcgtgtgca ctcgggtgat cgcgatcccg agcaaacctt
    17041 gccgtttttt ctggatgtga gcctgctgag tcggacgttg cgcgtcgagt cagaggcaat
    17101 cgaggctttc tttgcggagc tccgtcgaca aggagctcta acgccgcgcg ccatgttgaa
    17161 gcaggtttgc gacttcctgg agcggactga aaccggttcc ggggcgcgcg cgcgggagcc
    17221 ggatgtgggc aagccaccga gcggtgactc agcagtagat tgcctggagc gcctgtatag
    17281 agcagtggct cggcgctgtg cgggggctgg gcgcaaccgg tgttatcccg tggcaatcct
    17341 cgtcaacaac cagaggagtc gcgcgacttt ccacgtgcag cgcgatctgg ccgaagcctc
    17401 ggagcggcta accaacggcg agctcccagc tgagtctccg ctcactatat atatgactgg
    17461 gcgctcgccg gggttgcgcg cgcagccgtg ttacggacgc tggaaccgag cgcctttgac
    17521 ggatagccag cggacggcat tggagctcgc gctaggctct ccgttctgtg cggttcaagg
    17581 cccgccgggt accggaaaaa ctacactgat cttgaatgca gtggtcgaac agtgggtgca
    17641 aagcctccat gagctggccc gctctgggcg cccagcggag cgcttcctac tggtgacgag
    17701 tacgaataac cgagcagtgg acaacgtggt tgaaccgctc tccagtggag cgttggccga
    17761 ttgcccgtta gcccttcggt tgggcagccg tgaggtaacg gcaacgcaaa cgcgagcaac
    17821 cttggagcgg gtgctggcgt tcgtggaaag gcaagccaat gaagcggcgc tcgacgtgga
    17881 gcaggctcga gaacgcttcc gcagctgcta caccgaaatc gagcgcaagt ttgctccgca
    17941 gcgtctggtg caacaacagg agggcgaggt cggcgcaatc gatcgagagc tggcagacct
    18001 ctccaaagcc gcacgagcga gcgcgaacgc gctcgcgtcg gcgctatcga agttgaccgc
    18061 accgtccgtg agccctacct ccgaaaacca agcctccgac aacccgcgcg tgctcgacgc
    18121 cgccagctta catgtcgaag tgcagaacac gttacgcgcg ctcgggcgct tgagtcgaga
    18181 tactgaatcc gatctgcccg agatccgcgc acgcttcgag accggctatc gaaggctaca
    18241 caacaagcgg ttgaaggcgc tgtcacagtg cttcgagagg cgtatcgacc tgggcttgcc
    18301 gagcccagag gcggtgcggg cgcttacgtc agaggcctgt gagaccttgc tcgaaaacgc
    18361 ggtatccaaa ctcttggatt tggagtccgc gctgcgaagt gagcagcaag atcaagagcg
    18421 cacggagcgt cgagcagaga tcgagcagcg ttcgaaacga gcgcgcgcag agctcgctga
    18481 gctcaaggtg cgcgccgccg acccacccga gccgagcgag ctcgcgccgc tctccgccgc
    18541 gttgttcgat gcggcgctcg cgttgcgcct gagttgggtg cagagaaatc gcaaagaact
    18601 ggccgagagc ctgcgtgcgg caatcacggc ttgtgagcgg cagcgctcgt tacgtgtgct
    18661 gctcgattca cggtccggtg ccgggagcca cttgcgggcg ctatttccgg ccttcgggtg
    18721 cacgttgctg tcgatcggaa acgccttcgg acaagagacg agcaccttcg accgcgtcat
    18781 catcgacgaa gccggacaat gccacgcagc ctacgcggtt tcggcgttgt tacgggctcg
    18841 ctcggcgatt gtgctcggag atacgcacca gctcgagccc gtggttgggc tgaatcgcga
    18901 agacgagcga cgcatctcga ggagcttgaa gctgcgcatc gcggaagcaa agctgcagcc
    18961 gtttcggatg tatgatgagt ccggcaattc cgcgcagagc ctggccgagc gttgcactac
    19021 gcaggagggg atctccgagc gcccgacgct gcgggatcac ttccgttgcc aacccgagat
    19081 cgcagcgctt tgcgaggcgt ggtgtcagta cggcatgatc tcgcgcacgc cgcgaagctc
    19141 gaagagctcc ttcgcgccag agctgcgcgc ccccgtgctc ttcagcgcgg tcgcaggcca
    19201 gcaagagcgc agcatgggca gttacgtaaa tcgcgccgag ctagaggtcg tgacccgttg
    19261 ggtggagtac ctgttccgag ctggggttcc cgccgctgaa ctgggcgtgc taaccccgta
    19321 tcgcgggcaa ttgatcgcgt tgctggatgc ctttcgcgcg cggggcattc ccgtcgaacg
    19381 ccccgacgaa gagcccgacg agaacctcaa tctgttcggc acccagcccg atgggggcgt
    19441 cgcggtcggt accgtgcacc gcttccaggg cggggaacgc tcgatcatca tcttctcgac
    19501 cgctattacc gagccggcca gccttggatt tctggacgct cgcgtgcatt tgctcaacgt
    19561 agccgcgtcg cgcgcccgtg agcacttgtt ggtgttcggg cacgcagcca ctttggccgc
    19621 aggccggcat acccgcgagc tggtccgagc cggccagccc ttgccccggc ttccctacga
    19681 ctagcggaca gcgatctttg cgtcggactc ccaatcgctg accagaaccg tcgaggccat
    19741 caccgctacg ggaagtagca cgatcccgca gcaaggcagc cagaacagca agctgaacgc
    19801 cgtcgcgaaa ccgagcacct ccggaacgtg ttgccgcatg cgccgagagc gctcccgtgc
    19861 acccatgcct ctcaacgtga gcgggtaatc caagaggccc caagcgagac cgaaggcgct
    19921 aaccaagatc ttcaacggca ccgtgaccac ggccgcaccg gggaccacca tccccaacag
    19981 agtcagcaag agcatcagcg gcagtacgag caaggtcgca aacaaccagg cccggagccc
    20041 gcacgcaaac tcgcgccaga agccaagtgg cggaggcgca gcacgcccca gagaaacggt
    20101 ggtgccgtgc accaaccgct cgagcgccgg cgcggacaac atgggtgtga gcagcgccgc
    20161 caagaaccag cccaacaccg ccgaaagcaa caccgcgatc gcgctcaggc ccgtcgctgc
    20221 aactcgattt gcgattgggt gaccgaacac ctcaccactc aacacgcgag cttcgatcca
    20281 gggtgccgca cgttggatgg cgccccagat acaagccgcc tcgagcgcga ggaagacgag
    20341 gctcggtacc gccgcccagg gccagagcgc cgggcggctc agtagaatgc gaaaaccaga
    20401 gccgagcgtc cgcgcacctt tgatgaaacg agagccttgg gtcaccacgg atcctcagga
    20461 tatcagcaga acctgcgcgg gccgactctc gcggaacggg gctcgcattt actcatagca
    20521 gcctcaggaa ggcgagtaga gccgttcgct caccgggatc caagcgggct gcttcggcgg
    20581 gtgcgttggc atggaaggag cgctcgcggg tccaggcgaa gctctcgagc aagctttgga
    20641 gcgagtgcgc gctgccattg gtgaagtagg ggtacttccg gtccacgcgg cggaggctcg
    20701 ggatgcgtgt gccgagctcg tgaacgtacg gcaggacagc cgtcttctcg taatcacccc
    20761 gcgcccacac gacgggacca tcgtcccgga agatccattg cgaccaggtt ccaaatggaa
    20821 cctcgctgtc cggttcgtcg ctgaagagcc gcgccgcgtg gcagcccaca caacgacgcg
    20881 caaacacttc cgcaccggcc gcctcgatcg aggtgaacga agagcgcccg tgcgccaagg
    20941 cgttcgcgac atgagagttc ggagcgggct cgagcccagc taggaattcg agcagcccct
    21001 ggcgcagcaa ctctggagag aggtctgctg gcaacgcgcc gagccacgtt agccacggca
    21061 cccgctcgac actcgtagag aaccatggat tttgcggcgt gcccagagac gcgacgcgga
    21121 actcgttgtg cgccacttgc gtcaggtcgc gatccagggc gcgagagaag tgcggtgcgt
    21181 tctccagtaa ccccagcaat ggacgagtcg aaacgcgcac ctcaccgcgc ccggtatgat
    21241 gcacgcgacc gtcagtgcca gcctcgaagt ggcacgtctc gcaggtgaag cggctccgat
    21301 ggccggtgct gtccgcacgc ggcgccatca gctcggtgaa gaacagagcc tcgcccaaac
    21361 gtgcacttgg tgtttgctcc gaagcgccag ccacaggctg cactcgaacg cgaaagacgg
    21421 gttccgtgtc ggcgcgcacc cacgcatcca acaatgagct agcgccgagc cacgcgcccg
    21481 cgctcagcgg ttcgagggcg accagccctg gcacgccctc gaagcctttc gagttcggcg
    21541 gatcgagcaa gttcgagaac gaatagagta gccagttcac cgagccgtag ccggtaaccc
    21601 agagttcgtg cacggaacct cgggcaatcc agcgcagcgc cttcggcacc agaacgtggc
    21661 tctcggacac attcaacgcg tggattcgct gtgctgcgcg gcccaccact cggtagacgt
    21721 acacgaatga gtctacatat ccgaacgcac cctcgaagcg ctggaggggg tgatcttcga
    21781 cgccgctcgc cgcgacccaa agctcgtcgt tcaggggaag caagctgagc gcccagaatg
    21841 gaccgtcgtg ctcgatgctc gtgagagggg accgaacgcg cccgtgcgcg cttagcccca
    21901 ggaacaccaa ccgatgatcg gccaggcaat tgatcactag gtgcctggac gttgtctcca
    21961 gggcgatggg ttcgcgacac gtttcacgtc gctgcacgat ccgcggagta gtggatgcca
    22021 ggtccaattc gaagagcaga tgcgcgtctg catcggccac gaacgctgtg cgcccttcta
    22081 cggcgagggc gcggggcctg agtgcgcccg gcaactcgac cgtcgtaggc gagcccagct
    22141 cgatcgtcag cgagttccgc tcgtgagtcg acgaggcacg ctcgacaacg tgcgtgcgga
    22201 acacccaaag cgagtcagcc tgctcgccta ttacgagcag ctggccttct cgagttacag
    22261 cgcaacctac ggctccccgc gggcctgcgc gacgagacaa ctcgcgtcca gactcgtcaa
    22321 tcaccgccaa ttgcgaggtt ccgcgcagca cggcaatcca acgctgagaa tccaatcgac
    22381 agagccgatg aggatcaggg ccgctgtgcg tctttctcgg gacttccgga gaccgagcgc
    22441 gccgcaactc agactcgtgc gcgcgcagcc gttcgagagc cgcgtcaatc cttcgagccc
    22501 gcgtatcggc tttcccgctt cgttctaggc ccgagcttcg ttcggttgct gcgagttgct
    22561 ctgttcccga gcttcgctgg gtcttggagc ttcgctcaat tcctgagctt cgccgcgctc
    22621 cgtagctttg ctcagcaacc cccgtgtgac tggaatccga gcgggcgttg gcgcaaccaa
    22681 ggagcgtcga gttggtcaac gaaaccagag cccaaagaac gatgccggtt gcacttgcga
    22741 cgtgcatgag aaaattcgaa tgcagccgtt cgaggccacg ccgcgagctt gcacggattg
    22801 tctcgagcaa cgcaaaagtt tcgcgagcca gcctgagctt gccccacacg cactcgaggt
    22861 tacccgcatt ctgcaggttc aaggcagaac accagatggc accgtttggt ggcacgagca
    22921 gtgcacattg ggcctgcgga ggattgtgtc atgaggaatg tccctcgcgt acagcaatac
    22981 atgactccga gtccaatcac gattggtcgc aatcagccga tgagcgtggc ccatcgtttg
    23041 atgcgcgagc acgagatccg ccacttgccg gtactggagg cagggcgcgt gctcggcatc
    23101 gtgtccgcgg gtgatctaca cctcgtcgag acgctgcggg acgtagaccc aaaccgggtt
    23161 accgtcgagg aggcgatgac ggagaaccct tatacggtgc ccccgagcga accgctggac
    23221 cgggtggtga ccgagatggc cgagcacaag tacggctgcg cgctagtcgt ggatcaccac
    23281 aaagtagtgg gcattttcac gacggtcgat gcctgcaaag ccttcgccga tcacttggat
    23341 ggccgcaacc cgacctaacg caggctcctc ttgggagggc ctcaagagcg aacggatgcg
    23401 ctcgcgcgag gacgttccag aactggatcc accgcggtca gcactgccac ctgaatctcg
    23461 aggcgtcgcg acgggccaat cgccgagccg tcgagttcca cgatgtggaa gcgatcgtac
    23521 acgcgattgt tcacggtctt cacttgcgag ccgatgatct gaacccgttg attgtagagc
    23581 gcctgagaga gcgccagcag caaccccgac cgatctccgg tttccacttc cagagtggag
    23641 aaatcgccat ccgcattttc gatgaagcgc accacggtct cgcgcgaagc aggcgtgggc
    23701 gggacgcgtt gtccgtccac gaaacggcgg cgctccagcc gaccttcgag cagccccacc
    23761 agcgtctgcc gcaggcgctc gacctcttcc tgggtaacgg gtgcatggcg ctgctcgagt
    23821 gactcgtgcc gcacccaaaa tacgtctacg gcttcgtcgt gctgccgctc gatacggcgc
    23881 gtgtaggcct ccgcctcaat cacgtccagg ccgcacatca ccagcgccgc gctgattgtg
    23941 gccaacaagc caggccgatc gtccgccacg acacacaacg cgctccccgg cacgcgattg
    24001 gactcgaacg tgcaaactat tgccggcgat gccttgcggg ctagcgccag ctgcgcgtga
    24061 cagcgaatcg cggcagagtc gaagtttctg cgatagctct cgggcagcga ggccgcgaag
    24121 tctcgggcga actcatcgag cggcatgaca gttctcacgg caaggctggt catccaacgg
    24181 tcatccagcc tggagtttgg agctttgggg atttccgctc gatgtcgagc gagtctcgga
    24241 caggggataa gctgcttttg tgggactctt actacggcgc gggcggcggt ggcgtcgtgg
    24301 tacccggagc tggcgtgagc cccggaacgg tgaggcccgg gatctgcgga agaaaagtcg
    24361 gaagcgcggt cggaaatacc gtaggcaacg cggtcggaaa ccccgaaggc aacggtggca
    24421 aactgcttgg tagcgcgatt gaggggaacg gcaaggggtt cgtgccgaaa ggtggagtgg
    24481 acggtgtcga cgacgtgctc gtgctcacgg ggccaccgac cgcgggagtg gagggaggtc
    24541 gtgggcgcac gctcgtgggg ttcgggctcc cggctggtgg acgctccggc accacattat
    24601 cggggttggt tgcgggtgta gtcggaacca ccgcaacaga tggcgtcgtc gccggttggc
    24661 ccgtggcggg aacatcgaaa gccggcaaat ccggcacggt ccgccgtgaa ccgagcgtgg
    24721 attgggagag cacgaagcca atccccgcga tcagagcgac cgcgctgagc cccagccaga
    24781 tcagcgctcg gttgttgctc ctgcggcgac gttgcgttgc cgcacgcatc ggtggcggca
    24841 ccactcccgc gggaggaatc ggggccggcg cttgcgccgc aacgggcgct gcgggggaag
    24901 ccccaccgcc ccaaacgtgc gggggaggtg ccgcttgtgt gcccgttcgc cgcggcatct
    24961 ccccttcccc gtcgggcggc agcgtgtcgg gcagtttgtt cggacggtca ttgtccagag
    25021 tgtcgggcac ctgcggctta gccaaagagg ttcccgtgcc gagcacgacc gagcgctcct
    25081 cgcgcatcac ccgcgccgcg gtcgcggccg cacgacccgc ggcactcagc tcgcccgcgc
    25141 agcgtgatag ctcgctgcgc atcgcaaagg cggacgcaaa acgccggtct cgatcgggct
    25201 ccaacgcgcg gttcacgagc gagacgagcg actccggcaa gtccggcgcg agctcggcga
    25261 tgtgcttcac gtgacccgcg atgacgttgc cgacgatgac ctcgggggta tccccgtctg
    25321 ccggccgtgc tccggtcagc atctcgaaca acatcacacc caaggagtac aaatccgccc
    25381 gcgcatctac ctcgtgtgca gcatagaatt gctccggggc catgtattcg ggcgtgccca
    25441 tgactacgcc cgcgcgggta agttccttgc ggtattccgc cgctacccgc aacttggcaa
    25501 tcccgaaatc gatcagcttg agaacggggc cgcccggaga tggagtgacg aacacgttgt
    25561 ccggcttgag atcgcgatgc acgacggaca gctcatgcgc cgcttcgagg ccgacgagaa
    25621 tttgcaagcc gtagtccacg gcttgatccc tgccgatgcg accgacccgc gtgagcagcg
    25681 cctgcaaaga ctcgccgctc agcagctcca tcacgagata gggcgcaccg tccggcgtgg
    25741 tgtctacgtc cgtgacgcga gccacgtgcg ggctctggat ggtggccgaa acgcgcgcct
    25801 cttgcaggaa gcgcgcggac aggcccggac gtttggcgag cccctcgtgc aaaaacttga
    25861 gcgcgacggg gatgccgagc agttcatgcc gcgcctcgta aacgctgccc ataccgccgt
    25921 cgccaatcaa acggacgatg cggtatttgc cgtcgactag atcaccgacc gtcaggctca
    25981 tgggcgaacc ccatccacga agcgtgcgtc gagtgggaca taccggtcat attccctcga
    26041 aaacgtcagt gttctcggat ccgcgcgatc gacgaaaagc gtgccattca gatggtccgt
    26101 ctcatgctga acgaccacgg cgcgaaatcc ttcccattcg aagtcgagct ccgcgccggc
    26161 acggtcccag ccgcgcacgc gcacccgccg cgggcggcgc acgcgccccc ggatgccatt
    26221 gacggacaag cagccttcat agagaaagac cgcattttcg ggctggtcag catcgaccag
    26281 cggcgtgacc tggggattga ccagcgccat gagcgggatc gtctcggcct gggggtagcg
    26341 gggattgttc cggacctcga tcacgacgat tgccacggac tcatacactt gcggcgctgc
    26401 gagccccgcg ccatcggcgt cacgcatggt ctcgaccatg tcgtcgatca gggtctgcat
    26461 gcgtggcgaa gccagctctt cacgcgacac ttctcgagcc ctctgccgca gcacgggctc
    26521 tccgagctgc gcaatcttgc ggatcgccat ggcgcggatc ttacaccggc agctggcttg
    26581 cgcccgagct cttgctcagg actgcgctgc cagccacgga acatccggac ggccagcgag
    26641 ctgattggct cggcgagcca gtacgaacag cagatctccc aagcgattca ggtaaaccag
    26701 cacttctccg cgaactggcg cgacccgccc cgcagacaaa acacgtcgct cggcgcgacg
    26761 gcagatggtg cgtgcttgat ggagcaaggc cgcgactcgt gatcccgtgg ggaggatgaa
    26821 gctcgtcaat ggcggcaatt cggcctcggc gccgtcaatc gccgcctcga gcgcggaaat
    26881 ctcgcggtcg tcgagcaatc gcatgcgcag cttgtcttct ttcccgggca cacaagcgag
    26941 ttcggcgccc aaggcgaaca gcgtgttctg aaaagtcgcc aacatcgcgt cgatctcgtc
    27001 tggcagcgcg ctcggctggc ccgatctctc cgcgcgaatc acgccgatcg cggcattcaa
    27061 ctcatcgacg gtaccgtacg cttcgacgcg cgccgagtct ttgtccaccc gtgcaccgcc
    27121 gaacaagccc gtgctgccgt catcgccagt cttggtgtag atgcgcatgc ttttgcacgc
    27181 cagccatatc accctcgtag aaccggcgca tgcgctccca ggctcggagc gcccatgtct
    27241 agcccaaggc gcgcgacaac tcgttgtagc tctcgaccca gagcgcgcga tctcgcgcag
    27301 agccgtcgat gatctcgatg ccgaatcccg ggggaacggt cgcgctcccg aggttgttga
    27361 acggccgtcg ccaggcgacc ttccccacta ggcgcaccag ccggtcgagt cgcggaggcg
    27421 taagctccaa ccagacttcg tcctcgtccg gcaaggccag ggtgcgcacg tataaaccgc
    27481 gctcactgag gttgtacgaa tagccgtgct catccacttc ccgccccgcg gagcgaaaag
    27541 ccacgctgag gccgtaaggg atgcgtgggc tggtgcggcc gctgctgcga ccactcgtga
    27601 gctcgttggc gacgaagaga acattctcgg gcgacgcaaa gccgtcggtc acggttaccg
    27661 aggtgagccc ggtcaaagcg ctgcgcaaca cagaaatttc ccgcggcgcc gcgcacacca
    27721 cgaagctcgc ggagctgccc cgtccacgcg cggtcgtgac cacggcgcgc gcgtcgctca
    27781 tcaattcgct gttggccacg acgagcttca agctcggatc actggcgaag tgctctgcgt
    27841 cgtccgcggc cacggcgaaa cgaatgg
//
LOCUS       DASTKU010000099         4389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1134064, whole genome shotgun sequence.
ACCESSION   DASTKU010000099 DASTKU010000000
VERSION     DASTKU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134064"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFQ61_03570"
     CDS             <1..438
                     /locus_tag="VFQ61_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953554.1"
                     /translation="DVGIIEVRHGEIWTAFDGLGVGEEAFARLIRPEMRARISASNGS
                     TKERTIFRGLHELVLESLRRIDEGQVLPPPKLSATQLEAALASPDELAARVRELNVAA
                     RRLLMERNYDEAARALVRLSELDPSSHLVRANLEQLRRLGYPK"
     gene            435..1238
                     /locus_tag="VFQ61_03575"
     CDS             435..1238
                     /locus_tag="VFQ61_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET9953555.1"
                     /translation="MTQTIAFSSLKGGVGKTTTALNSAFAFARRGARTLLVDTDPQGA
                     IGLSLDGVGERPGLATALASLRAPLPGVVKTRLPELHLLPVGLVDTLDMDRFSQDARE
                     ADLLRRIVDRSQQDYDIVLFDTPAGLGGMTRLVLETVESVIAVVQCEPLALRSLPRLL
                     ELLARLREAGAECSLLGVVGNMSSFRDPVMLASLEELWALYRDRVFDTAIPRDNVFLQ
                     ASRAGVPLGLLSRRPPAVASVFDSLVAEIEARLGVTQGGDDGGPIRLVD"
     gene            1207..1938
                     /locus_tag="VFQ61_03580"
     CDS             1207..1938
                     /locus_tag="VFQ61_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953556.1"
                     /translation="MMADRSVSWTDAERVALLLRQVSSPRPPVPRNPQKNVAIPTGAS
                     SGAVRPVTGIPAPPLPTANVTRSSSPAPLPSAAPPLPSAAPPAIAAGAVPVISAGAPA
                     RAKAQNIASAAHEAVAVPRLQQTSDNPSDRLDALVAWTLDHYRCTGAFVADDNGLTLA
                     AHGISEAHVSLVGPLFSALIGIRSIPGIDGTAGALWLGANMMSWVETRTERGGFCLGA
                     VGPEALPTRALEQLKEALEITVRGL"
     gene            2011..2385
                     /locus_tag="VFQ61_03585"
     CDS             2011..2385
                     /locus_tag="VFQ61_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953557.1"
                     /translation="MIDNKILDLAVQDLRNILRDGLIATDIWDRAAGLSLAGFNQQPV
                     AVALFTRITQELESSLLDSNFPPLGRYYLLDMEGNHTVVMVNHGVLLQGILVDNKRAN
                     LGLLISVAIPRMLDAIARAKPR"
     gene            complement(2538..3302)
                     /locus_tag="VFQ61_03590"
     CDS             complement(2538..3302)
                     /locus_tag="VFQ61_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953558.1"
                     /translation="MREVMKYIFGRKKEYTQLPLFEWMRDDRLAPIDRLAFYPCMAPF
                     ILSFGDLNKFVLRSEPTNDPFQEMVNVHSYEDDHHWPWYLEDFTKLGFDAELRGTEWM
                     NFLWGEETQQNRVLMARLTGLIKGAGGLERLAIVEAIEETGNVLFGTMLPLAEALERQ
                     LGTQLRYCGTFHFERESGHSVGADHKAMVSVSLSESERTRFKASVDEVFSAFEAWTHE
                     LLRYGLAHPSPTTKFLEEASSSIWPRAGVSVNLKSG"
BASE COUNT          809 a         1316 c         1311 g          953 t
ORIGIN      
        1 gacgtgggca tcatcgaggt gcgacacggc gagatctgga ctgccttcga cggtctaggg
       61 gtcggcgaag aagcctttgc gcgcctgatc cgtccggaaa tgcgcgcaag aatcagcgcg
      121 agcaatggct ctaccaaaga acgcaccatt ttccgagggc ttcacgaact cgtgctcgaa
      181 tctctgcgtc gaatcgacga aggtcaggta ttacctccac ccaagctctc ggctacacaa
      241 ctcgaagccg cgttggcctc tccggacgag ctggcggcga gggttcgaga gctcaacgtt
      301 gcggcgcgcc gcttgctcat ggagcgcaac tacgacgagg ctgcgcgcgc gctcgtgcgc
      361 ctttcagagc tcgatccgtc ttctcacctg gtgcgcgcca acttggaaca gctacgcagg
      421 ctcgggtatc ctaaatgaca caaactatcg cattctctag cctcaagggg ggcgtgggaa
      481 agactaccac cgcgctgaac agcgcgtttg ctttcgcgcg tcggggggcg cgcacgttgc
      541 tcgtcgatac ggatccgcaa ggtgcgatcg gtctctcgct ggatggggtg ggggagcggc
      601 ccgggctcgc gactgcactc gcgagcttgc gggcaccact gcctggcgtc gtgaagactc
      661 gcttacccga gctacatctc ttgcccgtgg ggctcgtcga tacgctggat atggaccgtt
      721 tctctcaaga cgcgcgtgaa gcggatctgc tgcgccggat cgtcgatcgc agtcaacaag
      781 actacgatat cgttttgttc gatactccgg ccgggctagg cgggatgacg cgactggtac
      841 tcgagaccgt cgagagcgtg attgcggtgg tgcaatgcga gccgctggcg ttgcgatcgt
      901 tgccgcgtct gctcgagctg cttgcgcgct tgcgcgaagc cggtgcggaa tgctctttgt
      961 tgggggtcgt aggaaatatg agctctttta gagatccagt gatgctcgct tcgttggagg
     1021 agctgtgggc gctgtaccgc gatcgtgtat tcgacacggc cattcctcgt gacaacgtat
     1081 ttcttcaagc tagtcgagcc ggtgtgccgc tcggacttct gagtcggcga ccaccggcgg
     1141 ttgcatcggt gttcgatagc ctggttgcag agatcgaggc gcgtctgggt gtcactcaag
     1201 gaggcgatga tggcggaccg atccgtctcg tggactgacg ccgagcgcgt agcgttgctg
     1261 ttacggcagg tgtcgtcgcc gcgacctccc gttccgcgta acccgcagaa gaacgtggcc
     1321 atcccgactg gggcgagcag cggtgccgtg cgtcccgtta ccgggatccc cgcgccacca
     1381 ttgccgacgg ccaacgtcac ccgtagctca tcccctgcgc ccctgccttc cgcggctccg
     1441 cccctgcctt ccgcggctcc gcccgcgatt gcggcgggtg ccgtgccggt gatcagcgcg
     1501 ggcgcgcctg caagagcaaa ggcacaaaat attgctagtg ccgcgcacga ggccgttgca
     1561 gtgcctcgcc tgcagcagac gtccgacaac ccttcggatc gactagacgc actcgtggcg
     1621 tggactttgg atcattaccg atgtaccggc gccttcgtcg cagatgacaa cgggctgacg
     1681 ctagcagctc atggcatctc ggaggcacac gtttcactcg tcgggccgtt gttctcggcc
     1741 ctgatcggaa ttcgatcgat cccgggcatc gacggcacgg caggggcgct gtggctcggc
     1801 gcgaacatga tgtcgtgggt cgagacccgg accgagcgcg gcggattttg cttgggcgcg
     1861 gttggtcccg aggcgcttcc gactcgtgcc ttggagcaac tcaaggaagc actcgagatc
     1921 acggtgcgtg ggctctgagc ccgccgttcg cttctctcgt cgaatgctct catcaacgca
     1981 ggacagctag ctcacaagca cagacacacc atgatagaca acaaaatcct ggacctggca
     2041 gttcaagacc tccgcaacat tctgcgcgac ggcttaatcg ccacggacat atgggatcgc
     2101 gccgccggcc tctcgctcgc gggcttcaat cagcaaccag tagccgtcgc gctcttcacg
     2161 cgcatcaccc aggagctcga gagctctcta ctcgactcca acttcccccc gctcggacgg
     2221 tactatctgc tcgatatgga agggaatcac accgtggtga tggtcaatca cggtgtattg
     2281 ttgcagggca ttctggtcga caacaagcgc gcgaacctcg gtctactcat ctcggtcgcg
     2341 atcccgcgca tgcttgacgc gattgcacgc gccaagccgc gctgaggcta accaccgctg
     2401 tgctcaacca ccgctgtgtt caaccaccgc tgtgttcaat caccgtgccg ccgcttgacc
     2461 agtgcagggt ctaaccacca ccgtagtgcc caatcccggc ggcctctaat ccaaccggat
     2521 tccagccaac gaaaggctta ccctgatttg aggttcacac taacgcctgc ccgcggccaa
     2581 atggaggagg aggcttcctc gaggaatttc gtggtcgggc tgggatgcgc gaggccgtag
     2641 cgcagcaact cgtgcgtcca agcttcgaag gcggagaaca cctcgtccac cgaagctttg
     2701 aatcgggtcc gttcactttc agaaagcgat acgctcacca tcgccttgtg gtctgcaccc
     2761 acagagtgcc cggactcccg ctcgaagtgg aatgtaccgc agtagcggag ctgtgttccg
     2821 agctggcgct ccagcgcctc tgccaacggc aacatcgtac caaacagcac attccctgtt
     2881 tcctcgatgg cttctacgat cgccaagcgc tccagcccac ccgcaccttt gatcaggccg
     2941 gtcaggcggg ccatgagcac gcgattctgc tgagtttcct cgccccacaa gaaattcatc
     3001 cactcggtgc cgcgcaattc cgcgtcgaaa cccagcttcg tgaagtcctc taggtaccag
     3061 ggccagtgat ggtcatcctc ataggagtgg acgttgacca tttcctggaa gggatcgttg
     3121 gtgggttcgg agcggagcac gaacttgttc agatccccga agctcaagat gaatggagcc
     3181 atgcacgggt agaaggccaa tcgatcgatg ggcgccaatc gatcgtcacg catccactcg
     3241 aaaaggggta gttgcgtgta ctctttcttt ctcccgaaga tgtacttcat cacctcgcgc
     3301 attttagtat cctctcgtgg ttgggcgtct gagctcttgc tctgagctct tgctcgactg
     3361 gtgccaattg aagattggga aagcttcgct tcctacaacc gtgtgcgatt cgcaatattc
     3421 gtgcttcgcg ccgttcggtc tcctgagcgc cgcctccaat tcatttgggg gttcagccgt
     3481 cgttgggctc agttggctgg tcgttgggct cggttggtcg ttggactcag ttggtcgttg
     3541 gactcagttg gtcgttgggc tcagttggtc gttgggctca gttggtcgtt ggactcggtt
     3601 ggtcgttgga ctcggttggt gggcacgggc gtcgccggtg agtgccacct gtgcccacaa
     3661 aagtactctc acggatatcc gttggttttc ctggacaatt cttaacgaaa tcttttgttc
     3721 aatggccacg cggcttcgaa gatgacgtgc gcgcggcgct actcaggttt ctgtgactag
     3781 agccagccca ctctgcacgc gcaacactct ggaaaaagtc gactccattc aagagtgaca
     3841 tgcgtcaaaa atgtcgatcc gtattcactc gaatggtggc ctagccgaac tactacgaga
     3901 agagctgcca cgatggtgat tgcccggcaa cttgcctgac caaggactcc ggtgacaagc
     3961 acgctgcctg ataagcagct gcctgataac cacagtacct aacgggtgcc tttgtacgac
     4021 atctgtggct gatcctaggt aacttctacg tggcgctctc tcaatcggta tcagccgaag
     4081 agttgtaggg gatcgggagc tgaaggcgaa tgctgctcac atccagccaa tgggcttgcg
     4141 ggcccgttca agccgccttc gtgggtttgc actgctgagt tctccgccaa gtcttcagcg
     4201 cgagcggttg ggatagctcg ccattccgag ggttcgtctc cattggcttc gatgctgcaa
     4261 gaagcgttga cccgggccca tccgagtgat taagggccta tctctatagg accagttgtg
     4321 ggaactagcg ggctccatac tgcccgaggc tgtctctcga gcggtgatgt ggcgactttt
     4381 cgtgggcgt
//
LOCUS       DASTKU010000100        12402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_688261, whole genome shotgun sequence.
ACCESSION   DASTKU010000100 DASTKU010000000
VERSION     DASTKU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12402
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688261"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFQ61_03595"
     CDS             complement(<1..536)
                     /locus_tag="VFQ61_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953559.1"
                     /translation="MRARFVEVALSLFLGASESLGALESLPSGMFPNVLVSGAMLRAA
                     SVPATALALLLPRRVLAAPQEKQSKGARQRPAPRVLAPVAVRPENGSSELGGTSDRAA
                     TPVAPATRGPSVAGPPARVLLRGCEGMQESEVERVLSAELGTQSNSPPASSSSSAEPT
                     WIVVRCADGQITVEVNDPL"
     gene            823..2268
                     /locus_tag="VFQ61_03600"
     CDS             823..2268
                     /locus_tag="VFQ61_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9953560.1"
                     /translation="MGLMDFVKGGVGRMMIARPDEQKEKVIYKHPDQTFPFWSQLTVD
                     SDEVCLFFKDGTFVGALPPGRHTLQTQNIPFLNSLVDKFTGGNVFISELFFVTTRPLY
                     NQGFGDTIGSMRDPQLDIRVTPRAFGTYSFRVVDPVRFVIGFRGQTGGDPEQSMRWVR
                     DQLFMGLKSTLARLIKSGELTLLDLGTCGPDVARAVVQSCPDLANIGISVLEIAKLNI
                     NLSDEDQHRIDEAQDQIVQAKLDARKARIAVSQAEAEAQARQFKLDQDFMNRARYVNQ
                     MDVGRYQQYAAAEATLGLGEGLAQGGAGTAGGVVAAGIAQGIGLGAGLGMAGPAGQYA
                     QMPPGYPGYPPPGYPPPGYPPAGVGPGPGYPPPGYPAAPGQPVHPQGGHPAQGYPPSV
                     HQPPAYSQPVYPPGGPTMPGAFGPPPGYPPAAGTPGVPAVNPSNPGDPSTHRTELSAT
                     GTVSLGKCPGCGAANPSAAKFCSECGAKLGV"
     gene            2541..3989
                     /locus_tag="VFQ61_03605"
     CDS             2541..3989
                     /locus_tag="VFQ61_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9953561.1"
                     /translation="MRATDWDPLSDPLLDRVVDGRYQIEAVIGEGGMGTVYQVRHVLL
                     DKRFALKALRADLASDSEIAPRFIHEARAAASVSHPGLVQISDYGQLPTGQAYFVMEL
                     LEGMSLRVLLRRNGALPPARAVGIAQKIADALRAAHETGIVHRDLKPDNIHVRATRTG
                     DEVKVVDFGLAKIMGKSPLTQSGVVFGTPHYMSPEQAQGNPVDPRSDIYALGVVLFEM
                     LTGRVPFEADTYMGVLTKHIYMKPPAPSQRVTLGAAGEVLDDIVLTCLHKQPERRFQS
                     MAEVVARIEEIPAHVLPGLEPAQPNSSAAFASEILEPETLALRPRSSHLKWAAGALLT
                     VTCAGLGAWLVPKWFAEPAEPISDTAPTSIEGVQPATEFRRAAAGEQNEAGERQSSAI
                     ERQSAAQKQSAAPVASPATASQPFDGISAPSARDAEAQAQDSSAEPAAARGTAAPTAR
                     PVTRKVSAKAGQRPVRSKVAISEIVDPWAKTR"
     gene            4051..4359
                     /locus_tag="VFQ61_03610"
     CDS             4051..4359
                     /locus_tag="VFQ61_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953562.1"
                     /translation="MSEVGFGLTDREITGTLARVGVFPRLFESLLTDSLSFDHLGPVI
                     NFRVAMEHCGLFPAAGSAGSRYPGSRGVRASKTSGARLRIADAWGGRGGTARSKALVM
                     "
     gene            4361..7327
                     /locus_tag="VFQ61_03615"
     CDS             4361..7327
                     /locus_tag="VFQ61_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9953563.1"
                     /translation="MAKKQLLLVDADPRSVRVLEVSLRKAGYSVTTASDGQDALSKIE
                     FSAPDLILTDTRLPRIDGYELVRQIKSRPDYATIPVVFLTSQRSIEDKIRGLELGVED
                     YLTKPIFVRELIARVTLLLARTTHDRIAAMSVPVSRRTRLSGSLEDMGVVDLLQTFEL
                     SRKSGMGRIASGRREATIFFRDGKVVDAELGRLRGEEAVYRALVWNSGTFEVEFCPVK
                     NEDVIPTTTQGLLMEGMRRVDEWGRLLEQLPPLDTIFEIDRDALIERLTEIPDELNGI
                     LRLFDGKRTLLDVVDESPFEDLSTLSTITKLYFEGLLIIPPSVEEDVVPSVEQEPPRE
                     LVRTSEGEELVLGSEPRLPLVDSAAPPSGGRRASWRPSAPVIEPLALPGEIMPRTLAG
                     LGATLPETVVLEPASQAIPPPSSQPGGTVTGMLGSAGGARSTGSHATGSHPSSAAPPA
                     AKTSAGAVDELPEGAPRRTRTLTPPNMGPSPSAGGTHERAVETAAARAALQRLEAEAS
                     KPVVPAAGQGVPPAAVEMRPIHAVEQPAAALRALEGKSAAEGKVIPFPNRRDDEAPAT
                     DQPPPPVVTLPAADSLREREPADDPRQEPETSGRDDATYADRPAWEARRSDSQRPDPS
                     ETQRLDRGELGRHERVPPTGLHGDEIDRRYGSATLIGGTAENFLGRGEKPGRDEDSAH
                     AKGSGSFKDSAHAKDSAHAKDSAHAKESGHIKESPATPPVKEAAHHADAAKAVHAPLE
                     PEPVVWPRVDQKKRANAGEHAEQVASNAVLDGRDHDELHDRFFAADEEELYASAKGDA
                     VLESEFESGMEARRVRPPLTHAQQQRRVRSLRIVAAVIGFGIGVLAFAFFFWGRSTGE
                     DELGTGASDSASAPNTATAASGSPGTPQESPPPATPPEQAPPPPPAEPAANAAAEPDL
                     PSMALPDVAPTSEPVPTTTPQARPEPAKPAALAPALRNESRPAGRDTSSEPEAPKPPP
                     ARLPPGVKPPTAAFPL"
     gene            complement(7375..8253)
                     /locus_tag="VFQ61_03620"
     CDS             complement(7375..8253)
                     /locus_tag="VFQ61_03620"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9953564.1"
                     /translation="MTDRVVLITGASSGLGLAIARALLSHNYQLILTAREASLARFAA
                     HGIVESDRVMLRALDVASDSERKAVIAEANERFGGVDVLLNNAGISLRSVVEHVTEAD
                     RRLQMAINFEAPLELTRLVLPRMRKRRWGRILNISSVGGMMAMPTMSIYSASKFALEG
                     ASEALWYEVRPFGIYVTLIQPGFINSDGFENVRYTTMSRNAHDTVNSAYHWHYHYMAG
                     FIGRVARRVPATPDSVARTVLRTIEAKAPPLRVPATFDARVFSLLRRLLPRTLYHWVL
                     YNSLPQVREWGPRKPR"
     gene            complement(8671..9552)
                     /locus_tag="VFQ61_03625"
     CDS             complement(8671..9552)
                     /locus_tag="VFQ61_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9953565.1"
                     /translation="MAASGALTTEPGSVSAPVERDVTASGVRLRVVEHGTGQTVVFVH
                     SLFMDHRTWDGVRAAMQGEFRIITPDLPGFGQSEKPAPSRFPYGINAFSEAIADLYAG
                     LEIGRAALVGHGLGGAIALTLAARHPELLSHLVLVDPWVYPGRMGLELRLAHLPLISN
                     LIFKQLVGRGWFRTYFRNRLISPSASVDVQRIDSFYEVFNSPAARGSALATLSGCADT
                     RSVEAQTTRIRTPTLVIWGSQDELTSCRHGQRLAREIRGAGLELIDTGHSPQEERPTE
                     LAGLIRRFLRSPIPTFG"
     gene            9963..10472
                     /locus_tag="VFQ61_03630"
     CDS             9963..10472
                     /locus_tag="VFQ61_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953566.1"
                     /translation="MIDDAALNQIEKSHSRGLTSSEILDLFARHNIALSEATLRKYVQ
                     LGLLPRSVRIGKKGKHTGSQGVYPVGVVRQILLIKEMMAKSYTIEQIQKEFLFIRNDI
                     EHLEQLLEGIFSKLDGVVRQRRSEATTQVVVRELGDARSLSRDLMTRLSSIETRLTRR
                     ARMETIAAS"
     gene            10702..11352
                     /locus_tag="VFQ61_03635"
     CDS             10702..11352
                     /locus_tag="VFQ61_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET9953567.1"
                     /translation="MDSNNSHTPDETTKPARLPTDGALALEAETDEAQITREQRRSRR
                     KRDVRARTISVKRMTKRELEIGRLLYPEADYWKPRTRAECLEGPRPCPFVSCKHHLYI
                     DVSPRTGAIKLNFPDLEVWDLGESCALDVADRGGTTLEDVGAIMNLTRERIRQVEVKA
                     LAKLEALRDMMALRDYVDEGPVGKRRLPRIADAELTSEADDDDEEVAVKKAPRIDA"
     gene            complement(11582..>12402)
                     /locus_tag="VFQ61_03640"
     CDS             complement(11582..>12402)
                     /locus_tag="VFQ61_03640"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET9953568.1"
                     /translation="STNPLRAASERFEVLLENLTAFKTLLARESAELVHVRTFAEYRA
                     ARETGRHAAFIGIQGGNACDGRADSVESLPRLDVLRVTLVHLSNSSIGSSSAPPGSWA
                     PRGLTDYGRRFIERLNAARIFVDLAHISPAGFWEALDAHDHRLPVIVTHTGVSGVHRH
                     WRNLDDSQLRAIAATGGLSGIMVHAGYLGPGLFGARVEHVLQHIEHAVRVAGEDSVGI
                     GSDFDGFIVPPRDLPSVIHYPRLIQAMLDRKMPESRIRKILGGNFLRVLRELRP"
BASE COUNT         2327 a         3926 c         4000 g         2149 t
ORIGIN      
        1 agtggatcgt tgacctccac cgtgatctgt ccgtcggcgc agcggacgac aatccaagtg
       61 ggttcggcgg aactcgagct ggaggcggga gggctgttgc tttgggtacc gagttctgcg
      121 ctcaagacgc gctcgacttc cgattcctgc attccttcgc agccgcgtag caacactcgt
      181 gcaggcgggc ccgctaccga cgggccgcgt gtcgcgggcg ccactggcgt tgcggcacgg
      241 tctgatgtgc cgccaagctc ggacgagccg ttctctgggc gaacggcgac cggcgccaga
      301 actcgcggtg cgggacgctg acgcgcccct ttggattgct tttcctgggg agctgctagc
      361 acccgccgag gcagcagcag ggccaaggcc gtggctggaa cgctggcagc acgaagcatc
      421 gcgccggaca ccaaaacatt gggaaacatc ccgctgggaa gcgactcgag agctccgagc
      481 gactcggaag caccaagaaa tagcgagagt gcgacctcca cgaaccgcgc gcgcatgccc
      541 aacccaaaga ctgttaccac gcaggcggct cgcgagggaa ctccctcgaa gctaattcag
      601 acaaagccaa ttcaaccaag tctggccccg agccattccg ggcattcgac gagtcgagcg
      661 ccggccccga gcgggtgcca gtgaagaggt gcttgccgct gctgagcgct gcacggaaaa
      721 acatttgtcg acgggtgttc ggcggtcagt tggagtacgc ttcgcgaacg tcacgcagtg
      781 gtccgctcgt ctcgcggcgg gcgcacccaa caggagagaa tcatgggatt gatggatttc
      841 gtgaagggcg gcgtgggccg gatgatgatt gctcggcccg acgagcaaaa agagaaggtc
      901 atctacaagc atcccgacca gacgttccct ttttggagcc agctcaccgt cgattccgac
      961 gaagtctgtt tgtttttcaa ggacggcacc ttcgtgggtg cgttgccgcc ggggcgccac
     1021 acgctgcaga cgcagaacat tcccttcttg aactcgctgg tggacaaatt cacgggcggc
     1081 aacgtcttca tcagtgagtt gttcttcgtg accacgcggc cgctctacaa ccaaggcttc
     1141 ggggatacga ttggttcgat gcgcgatccg cagctggata tccgggttac cccgcgcgcg
     1201 ttcggcacat actcgttccg agtggtggac ccagtccggt tcgtaattgg gttccgcgga
     1261 cagaccggtg gcgatcccga gcagagcatg cgttgggtga gagaccagct gttcatgggc
     1321 ttgaaatcca ccttggcgag gctgatcaag tccggagagc tgacgctctt ggatctgggc
     1381 acttgcggtc cggacgtggc gcgcgccgtc gtgcaaagtt gcccggattt ggcgaacatc
     1441 ggaatttcgg tgctcgagat cgccaaactc aacatcaatc tgagtgacga ggaccagcat
     1501 cgaatcgacg aggcccaaga ccagatcgtg caagcgaagc tcgatgctcg taaggcgcgg
     1561 atcgcggttt cgcaggccga ggcggaagcc caagctcgac agttcaagct cgatcaagat
     1621 ttcatgaacc gcgcccgcta cgtaaatcag atggacgtgg ggcgatatca gcaatacgcg
     1681 gccgccgagg ccacgctggg ccttggcgaa gggctcgccc aaggtggagc aggaacggcc
     1741 gggggcgtgg tcgcggcggg cattgcgcaa ggcattggtc tcggtgcagg gctcggcatg
     1801 gcgggtcctg ccggtcagta cgcgcagatg cctcccgggt accccggcta tccgcctccc
     1861 ggctatccgc cacccggtta tccacctgct ggggttggtc caggtcccgg ctatccgcca
     1921 cccggttatc cggctgctcc ggggcagcca gtgcacccac aaggcggcca tcctgcgcag
     1981 ggttacccac cgtccgttca tcaaccgccc gcctattcgc agcccgttta cccgccggga
     2041 ggccccacca tgcccggcgc tttcgggcca ccgccagggt atcctcccgc agccggaacg
     2101 ccgggcgttc ctgcagtgaa cccgagcaac cccggagatc cctccaccca tcgcactgag
     2161 ctttctgcga cgggaaccgt cagcctgggg aagtgcccag ggtgtggcgc ggcaaaccct
     2221 agcgctgcca agttttgctc cgagtgcggt gcaaaactgg gggtttgaaa gagtcgctgg
     2281 ctctgagaca atagcggtcg caagcttgtc aggtcgaccc gagcttcggt cgatcgactg
     2341 aagtgcttcg gcttcttgaa gcgccccgtc ctcgagttgc gatcctaagt tgctgagccg
     2401 gtccgttgga aacccaagcc cacgtctccc cgtcgaagcc gccgagtacg tcagaagctg
     2461 actgccccac gcgctgccca cggtgcggtc agcatttttc acctgacggc tgcttctgcc
     2521 cgttcgacgg ggagcccttg gtgcgagcta cggattggga tccgctcagc gacccgctgc
     2581 tcgaccgagt ggtggatggt cgttaccaga tcgaagcggt gattggggaa gggggcatgg
     2641 gcaccgttta ccaggttcgc catgtgctcc tggacaagcg cttcgccttg aaagccctac
     2701 gcgcggactt ggcgagcgac agcgagatcg cgccgcgttt cattcacgag gctcgcgcgg
     2761 cggcgagcgt gtcgcatccg ggcctggttc agatctccga ttatgggcag ctcccgaccg
     2821 ggcaagcgta cttcgtaatg gagttgttgg agggcatgtc gttgcgcgtt ttgttgcgac
     2881 gcaacggcgc tctgccgccc gcccgcgcgg ttggaatcgc gcaaaagatc gccgatgcgc
     2941 tgcgggccgc gcacgagacc gggatcgtgc atcgcgacct caagcccgac aacatccacg
     3001 tgcgcgccac gcggacgggg gatgaggtca aggtcgtaga cttcggatta gcgaagatca
     3061 tgggcaagag cccactcacc cagagtggag tcgtattcgg aacgccccat tacatgagcc
     3121 ccgaacaggc tcagggaaac ccggtggatc cccgctccga catctacgcc ctcggagtgg
     3181 tgttgttcga gatgctgacg ggccgcgtgc cgttcgaggc ggatacgtac atgggggtgc
     3241 ttaccaagca catctacatg aagcctcccg cgccgagtca gcgagtcacg ctcggggcgg
     3301 ccggtgaagt gctggacgac atcgtcctta cttgtttgca caaacagccg gagcgccgat
     3361 tccagagcat ggcggaagtg gtggcgcgca tcgaagagat cccggctcat gtattgccag
     3421 ggctagagcc cgctcaaccc aactcgagcg ccgcattcgc gagtgaaatt ctggaacccg
     3481 aaacgctcgc gctcagaccg cgcagcagcc acctcaaatg ggctgcgggc gcgctgttga
     3541 cggtgacctg cgccggtctg ggggcgtggc tcgtgccaaa gtggttcgcc gaaccggcgg
     3601 aaccgatttc cgataccgcc cccacctcga ttgaaggcgt gcagcccgcg accgagttcc
     3661 ggcgcgcggc cgcgggcgag caaaacgagg caggggagag gcagagcagc gcgatcgaaa
     3721 gacagagcgc ggcccaaaaa cagagcgcgg ctcccgttgc ttcacccgcg actgcgtcgc
     3781 agccattcga cggcatatcc gctccatcag cgagagacgc ggaagctcag gcgcaggact
     3841 catcggcaga accagcggcc gcccggggca cggctgcgcc gactgcacga cctgtcacgc
     3901 gcaaggtgtc agcgaaggcc ggtcagcgac ctgtacgcag caaggtggca atatcggaaa
     3961 tcgtcgatcc ttgggcaaaa acgcgttgag gccgagccgt atcttacgcc cgtggactct
     4021 ctgaggagcg actgtggctg gggcgtctcc atgagcgagg taggcttcgg cttgacagat
     4081 cgagaaatca ccggaaccct tgctcgagtc ggggtgttcc ccaggttgtt cgagtctctg
     4141 ttgaccgatt ctctgtcgtt cgatcatttg ggtccggtaa tcaattttcg agtcgccatg
     4201 gagcattgcg gtttgtttcc tgcggcgggc tcagctggat cccggtatcc gggttcgcgc
     4261 ggtgtgcggg cgtccaagac ttcgggcgcg cggcttcgca ttgcggatgc ctggggcgga
     4321 cgcgggggta ctgcgcgatc gaaagcgctg gtgatgtgac gtggcgaaga aacagctcct
     4381 tctcgtcgat gccgacccgc gaagcgtgcg cgtgttggag gttagcctgc gaaaggctgg
     4441 ttacagcgta actacggcct cggatggtca ggacgcgctc tcgaagatcg aattttccgc
     4501 gccagacctg attttgaccg acacgagatt gccccgcatc gacggctacg aactcgtgcg
     4561 gcaaatcaag tcgcgcccgg actacgcaac gattccagtc gtcttcctga ccagccagcg
     4621 ctcgatcgag gacaagattc gagggctcga gctgggggtg gaggattacc ttacgaagcc
     4681 catcttcgta cgtgagctga tagctcgcgt gaccttgctg ctggctcgca cgacgcacga
     4741 tcgcatcgcc gccatgagcg tgccggtgag tcgtcgcacc cgcttgagcg gttcgctcga
     4801 ggacatgggt gtcgtcgacc tgctgcagac cttcgagttg agtcgcaaga gcggcatggg
     4861 gcgaatcgcc tctggacgcc gtgaggccac gatcttcttc cgcgacggca aagtcgtcga
     4921 tgccgagctc gggcgtttac gtggcgagga agcggtttat cgcgcgctgg tctggaactc
     4981 tggaactttc gaggtggagt tctgcccggt caaaaacgag gacgtcattc ccaccacgac
     5041 gcagggcctg ctgatggagg ggatgcgtcg agtggacgaa tgggggcgtc tgctcgagca
     5101 gctgcctccg ctcgacacga tttttgaaat cgatcgtgac gcgttgatcg agcgcttgac
     5161 cgagatcccc gacgagctga acggaattct gcggctgttc gatggcaagc gaacgttgtt
     5221 ggacgtggtg gatgagtctc ccttcgaaga cttgtccacg ctctccacga tcacgaagct
     5281 ttacttcgag ggcttgctca tcatccctcc aagcgtggag gaagacgtgg ttcccagcgt
     5341 ggagcaggag cctccgcgcg aactcgtccg tacgagcgag ggcgaggagc tggtgcttgg
     5401 cagcgagccg cgtctgccac tcgtcgactc ggcggctccg ccgtccggtg ggcgacgggc
     5461 gtcctggcgt ccttctgctc cggtcatcga acctctcgct ttacccgggg aaattatgcc
     5521 ccggacgctg gccgggctgg gggccacgct gccagaaacc gtcgtgttgg agcccgcgag
     5581 tcaggcgatt cctccgccct cgagccaacc gggaggtacg gtcacgggca tgttgggttc
     5641 ggcgggcggc gcgcgctcga ctggctccca tgcgaccggc tcgcacccga gcagcgcggc
     5701 tccaccggcc gcgaagacga gtgcgggcgc tgttgacgag cttcccgaag gggcaccacg
     5761 gcggacacgc acgttgacgc cgccgaatat ggggcccagc ccttcagcgg gaggcacgca
     5821 cgagcgtgcg gtcgagaccg ccgcggctcg agccgccctg caacgtctcg aagctgaggc
     5881 gagcaaaccg gttgttcccg ctgccggtca aggtgttccc cctgccgccg tggaaatgcg
     5941 cccgatacac gcagtggagc agccagccgc ggcgctacgt gcgttggaag gcaagagcgc
     6001 cgcggaaggc aaggtgatcc catttccaaa ccgccgggac gacgaagccc ctgcgacgga
     6061 tcaacctccg ccgcccgtgg tgaccttgcc cgccgcggat tcgttgcgag agcgggaacc
     6121 cgccgacgat cctcggcaag agccagaaac ttcgggtcga gacgacgcga cctacgcgga
     6181 tcggcccgct tgggaagcgc gtcgctcgga ctcgcagcgt cccgatccct ccgagacgca
     6241 acgcctggat cgaggggagc tcgggcgcca cgagcgtgtg ccccctaccg ggctacatgg
     6301 ggacgagatc gaccggcgct acgggtctgc caccctgatc ggaggcaccg ccgaaaactt
     6361 cttggggcgc ggcgagaagc caggtcgcga cgaagactct gcccacgcga aaggctctgg
     6421 ctctttcaaa gactctgctc acgccaagga ctctgctcac gccaaggact ctgctcacgc
     6481 caaagagtca ggccacatca aagagagtcc cgcgactccg cccgtgaaag aggctgctca
     6541 ccacgcggac gccgccaagg ccgttcacgc accgctcgag ccagagcccg tggtctggcc
     6601 gcgcgtggac cagaagaagc gcgcaaacgc gggagagcac gcagaacaag tggcgagcaa
     6661 cgctgtcctc gatggccgcg atcacgatga gcttcacgac cgctttttcg cggccgacga
     6721 agaagaactc tatgcatccg cgaagggtga cgcggtgctc gaatcggagt tcgagtccgg
     6781 aatggaggcg cgtcgtgttc gcccaccgct gacgcacgcg cagcagcaac ggcgcgtccg
     6841 atcgctacgc atcgtggcgg ccgtgattgg tttcggcatc ggcgtgctgg cttttgcgtt
     6901 cttcttctgg ggaagatcga ccggggaaga tgaactaggc acgggtgcgt cggactcggc
     6961 ctctgcgcca aacaccgcga ccgcagcatc gggcagtccc ggtacgccgc aagagtcgcc
     7021 gccgcccgcg acgcccccag agcaagcgcc gcctccgccg ccggccgagc ccgccgcaaa
     7081 cgctgcggcc gagcccgatc tgccgtcgat ggccctgccg gacgtggcgc ccaccagcga
     7141 accggtcccg acgaccacgc cgcaagcgcg tcccgagccg gccaagcctg ccgcgctggc
     7201 gcccgcgctg cgcaacgaat ctcgccctgc gggtcgtgac acgagcagcg aacctgaagc
     7261 tcccaagcct ccaccggcga ggttgccgcc gggggtaaag ccgcctactg cggcctttcc
     7321 cctgtgaccc gacggccctc tgcgggcttt ccggctcttt ccgaaccggc cacgttatct
     7381 aggctttcgg ggtccccact ctcggacttg cggcaagctg ttgtacaaaa cccagtgata
     7441 aagcgtgcgg ggcagtaagc ggcgcagcaa cgaaaacacc cgcgcgtcga aggtggcggg
     7501 gactctcagc ggcggcgctt tggcttcaat cgtacgcagc acggtgcggg cgacactatc
     7561 cggtgtcgcc ggcacacgcc gcgcgacccg gccaatgaag cccgccatgt agtggtagtg
     7621 ccaatggtac gccgagttga cggtgtcgtg ggcgttgcgg ctcatcgtcg tatagcggac
     7681 gttttcgaag ccgtcggagt tgatgaaacc gggttgaatc agcgtcacgt agatgccgaa
     7741 cggacgcacc tcgtaccata acgcttcgct agccccttcg agcgcgaact tggacgcgct
     7801 gtagatcgac atcgtgggca tcgccatcat gccgccgacc gatgagatgt tcagaatgcg
     7861 cccccagcgt cgtttgcgca tgcgtggcag caccagacgc gtgagctcga gcggcgcctc
     7921 gaagttgatg gccatctgca gccgtcgatc tgcctccgtc acgtgttcga ccaccgagcg
     7981 cagagaaatg ccggcgttgt tcagcagcac gtccactccg ccgaagcgct cgttggcttc
     8041 cgcgatcacc gccttgcgct cactgtcact cgccacgtcg agcgcgcgca gcatgacccg
     8101 atccgactcg acgatgccgt gcgccgcaaa gcgcgcgagc gaggcttcgc gagccgtgag
     8161 aatcagctgg taattgtggg agagcagcgc acgcgcgatt gcgagtccga gcccagagct
     8221 cgcgccagtg atgagcacga ccctgtccgt catccggcaa agataacttg ataatgcgtg
     8281 tgcagccggc gcgcggcgcc aagaacgccg cttacatgct cttacgagca ggtgcctagt
     8341 gtttctcgct ccgttcggcc aatgacgcca ttggctatct tcgtcgcgcg actcatcgag
     8401 tctgctcggt cgaccccgac tattcgatcg tgcggtccga atcgatgcgt cgcacgcccg
     8461 agcctacccc gacgaagggc cccgcgtgag cccgggcttc tcgccggttg cccgcaccga
     8521 gcccggcagg gtgagcctcc agccgtggtg ctgcagcacc agaaacagcg caggccggca
     8581 cgagacaccc cagaaacacg agagcaagtc gaatcagcac gcgcgaaagg ctagttggaa
     8641 gcccctgatg cgggccaagt tcgcggcgct tcaaccgaag gtcgggatgg gagagcgcag
     8701 gaaccttcgg atcagccccg ccagctccgt cggccgctct tcttggggag aatgcccggt
     8761 atcgatcagc tccaaacccg cgccgcggat ttctcgcgct aagcgctggc cgtgtcggca
     8821 cgaagtgagc tcgtcttggc tgccccaaat cacgagtgtc ggcgtgcgga tgcgagtggt
     8881 ctgtgcttct accgagcggg tatctgcgca gccggagagg gtcgcgagcg cgctcccgcg
     8941 cgcggccggg gagttgaaca cctcgtagaa ggagtcgatg cgctgcacgt cgacgctcgc
     9001 ggaaggcgaa atcagacggt tacggaagta ggtacggaac cagccgcgcc ccaccagctg
     9061 tttgaaaatc aagttgctga tcagcggcag gtgggccagc cgcagctcga gccccatgcg
     9121 acccgggtac acccagggat ccaccaggac caagtgcgag agcagctccg ggtgccgcgc
     9181 ggccaaggta agagcgatcg ctcccccgag cccatggccg acgagggccg cgcgtccgat
     9241 ctcgagccct gcgtataaat ccgcaatcgc ctccgagaac gcgttgatgc cgtaaggaaa
     9301 gcggctcggc gctggcttct cgctctgacc gaagcccggc agatcgggtg taatgatccg
     9361 gaactcaccc tgcatagctg cccgcacgcc gtcccaggtg cggtggtcca tgaacaggct
     9421 gtgcacgaac accacggttt gacccgtgcc gtgttcgacg acgcgcagac ggacaccact
     9481 cgcggtgaca tcccgctcta cgggggccga gacggaaccc ggctcggtcg tgagggcacc
     9541 cgatgcggcc atggggcgcc aagcctactc gggttctggt ccatgggaac gcgaaatgca
     9601 ggccggcccc ccacgcaaac cgaccaaatc gcacacccgc gggctggtga ttcgtgtcaa
     9661 gcggacggcc ggagaggcga gtgccgccag tctgcggcct ccgagcgcca atgcgcgcag
     9721 gagcagtttt cgcgctccgg cgcggctcat ggacgcttta tgagctccat ggacaatcta
     9781 ccagaacgga cggaatatgt cctatctcgg acatcgcccc gcagccacag cgctttccgc
     9841 gcgatttcgc aaattttcga aggttaagtg ctttggacga tccactgagt ggaatatatt
     9901 cgcagccgct cgacgccccg ccgagtcggt tcggtggcgg cctcgggtcc cgggaatgac
     9961 tcgtgatcga cgacgccgcc ttgaatcaga tcgaaaagag ccactctcgg ggccttactt
    10021 cctcggagat tctggatctt ttcgcccgtc acaacatcgc gctgagtgag gccacgctgc
    10081 gcaagtacgt gcagctcggc ctactgccgc gcagcgtacg cattgggaag aagggcaagc
    10141 acacgggctc tcaaggggtg tatccggtcg gcgtcgtacg tcagattctc ttgatcaaag
    10201 agatgatggc caagagttac accatcgagc agatccaaaa agaattcttg ttcatccgta
    10261 acgacatcga acatctcgaa cagctgctcg aggggatctt ctcgaagctc gacggcgtgg
    10321 ttcggcaacg ccgcagtgaa gcgaccactc aggtcgtcgt gcgggaactc ggtgatgcgc
    10381 gctcgctcag ccgcgatttg atgactcggc tttcgagcat cgaaactcgg ctgacccgca
    10441 gggcgcggat ggaaaccata gcggcgtcat agcgctgact gtggtgttct gtgaatttct
    10501 tagcgcaggt ttcttaagtc tcaacgggaa gtgaagcggc agagcccgaa cggcaggttc
    10561 gtcgggcacg tcgatcgcca gggcttcgca tcccaaccag gtatcgaatc acgtgtgccg
    10621 ggccggcttc cggacgcgcg tctcccttcg ttcgtcaaga gcacagcgtt cgtccaaaga
    10681 gcacaggatt ctggaggcag gatggattcc aacaactctc acacccccga cgagacgacc
    10741 aagccggcgc ggctcccgac cgacggagct ttggcgttgg aggcagaaac tgacgaggcg
    10801 caaatcacgc gcgagcagcg tcgctcgcgg cgcaagcgcg acgtgcgcgc gcgcaccatc
    10861 agcgtgaagc ggatgaccaa acgcgagctg gagatcggcc gtctgctgta tccagaggcg
    10921 gactactgga aaccgcgcac acgggccgag tgcttggaag ggccacggcc gtgcccgttc
    10981 gtctcctgca aacaccacct ttacatcgac gtttcgcctc gcaccggggc gatcaagttg
    11041 aacttccccg atctcgaggt gtgggacctc ggcgagtcgt gcgcgctcga cgtggcggat
    11101 cgcgggggaa cgacgctcga agacgtcggg gccatcatga accttactcg cgaacgaatc
    11161 cgtcaagtcg aagtcaaagc tctggccaaa ctggaggccc tgcgcgacat gatggcgctc
    11221 agggattacg tggacgaggg gcccgtaggg aagcgccgcc taccgcgcat cgccgacgcc
    11281 gagctcacga gtgaagccga cgacgacgat gaagaagtcg cggtgaagaa ggctccgcgc
    11341 atcgacgctt gagttcctgc tccgcagttc ctgctccaca gctcctgctc cacagctcct
    11401 gctccacagc tcctgctcga cagttcctgc tccacagttc ctgctccaca gttcctgctc
    11461 cacagttccg gctccacctg ttcgagatct agcttgagcc ctgtgctgta ccgccgccgc
    11521 agagatgcgg cggtgttgtt tttgggggaa gcgcgcggcg ctacgcgcgc ggcgagtcag
    11581 gtcaagggcg cagctctcgc aggacgcgca agaagttgcc gcccaggatc ttgcggatgc
    11641 ggctctctgg catctttcga tcgagcatgg cctggatgag gcgagggtag tgaataacgc
    11701 tcgggaggtc gcgcggagga acgatgaagc catcgaagtc gctgccgatt ccgacgctgt
    11761 cttctcccgc cacacgcacg gcgtgttcga tgtgttgcag cacgtgctcg acccgcgctc
    11821 cgaatagccc aggaccgagg tagccggcgt gcaccatgat tcccgagagg ccgcccgttg
    11881 ccgcgatcgc ccgcagctgc gagtcgtcca aattgcgcca gtgccggtgc acgccggaaa
    11941 cacccgtgtg ggtgacgatc accggcaatc gatgatcatg ggcgtccaac gcctcccaaa
    12001 atccagcggg ggagatgtgg gccaaatcca cgaagatgcg ggccgcgttc aggcgttcga
    12061 tgaagcggcg cccgtagtca gtgagcccgc gtggtgccca actgcctggc ggcgcactgc
    12121 tgctcccaat cgatgaattc gacaggtgca caagcgtgac acgaagcaca tccaatcgcg
    12181 gtagcgactc cacgctgtcg gcccgcccgt cgcaggcgtt gccgccttga ataccgatga
    12241 atgccgcgtg ccgaccggtc tcgcgcgcgg cgcgatactc cgcgaacgtg cgaacgtgca
    12301 cgagttcggc tgattcccgc gcaagcagcg tcttgaacgc ggtcaggttc tcgagcagca
    12361 cttcgaagcg ctcggatgcg gcgcgcaggg ggttcgtgct ga
//
LOCUS       DASTKU010000101         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_466227, whole genome shotgun sequence.
ACCESSION   DASTKU010000101 DASTKU010000000
VERSION     DASTKU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466227"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..586)
                     /locus_tag="VFQ61_03645"
     CDS             complement(104..586)
                     /locus_tag="VFQ61_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET9953569.1"
                     /translation="MDNVELFLAHAVQLERDAARRFEDLMHSMHSAGNYEVEALFRRL
                     GEFSRLHLKAATARSGFQSLPALQPHEFQWPEGTSPEAARWRGVDGALDVLSALELAL
                     EGEECGMRFYEATATSSGNPEVVRMAREFASEEADHVSELQRWIQRATTGQTAPQKHY
                     "
     gene            complement(733..1047)
                     /locus_tag="VFQ61_03650"
     CDS             complement(733..1047)
                     /locus_tag="VFQ61_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET9953570.1"
                     /translation="MLTKKHVFVCVQNRPAGHPRGSCQSKGSPSLYQAFSDEFTRRAL
                     WNDYRLTLTNCLGPCENGPSVLVYPEGVLYGHVGAEDVAPIVDQHLIAGSPVERLAVA
                     DW"
     gene            complement(1159..>2527)
                     /locus_tag="VFQ61_03655"
     CDS             complement(1159..>2527)
                     /locus_tag="VFQ61_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9953571.1"
                     /translation="QEALETDTDRALDASLVAVPIKDAGRFIGVLAIERSHGPGDQAC
                     FCFDADVRLLTVIAHSIAQASRASELLGTRAIQSKAFESKETETKGVEEPAPILTPSD
                     AAASQLPRTSPAAKHGASTMSGGHPRWRATVDRARIAAKSSSTVLLRGESGTGKEVLA
                     RYIHGMSQRVQRPFVSVNCAALAESVLESELFGHERGAFTGASAQRKGRFELADGGTL
                     FLDEIGEISGAFQARLLRVLQLGEFERVGGMHTLKVDVRVLAATNRNLEEEVRTGRFR
                     ADLYYRISVVPLFLPALRERASDIPELATEFLASFNESNGTRLVFDPSALAALQDYSF
                     PGNVRELENAVRRAASLASGSRLTARDFEWLEQDTPLTLALRETQSPPPPSNREPRLQ
                     GGHSTSSGELPLRRLGEDASRERLIAALEQAGWVQAKAARLLGLTPRQIAYALQKYQI
                     PIRRF"
BASE COUNT          443 a          792 c          808 g          484 t
ORIGIN      
        1 ggacgagccc gcaaactcca ccaacttgta aacgtgattg gccatgcgca gctactcgct
       61 tccctttctc tcgcgcaggg gtcgcccggg cggcccctgc ttttcaatag tgcttttgag
      121 gtgccgtctg ccccgtcgtg gcgcgctgaa tccagcgctg caattcactg acgtggtcgg
      181 cctcttcgct cgcgaactcg cgggccatgc gtaccacctc ggggtttccg gagctcgttg
      241 cggtggcctc gtagaagcgc attccgcact cctctccttc gagggcgagc tccagcgcgc
      301 tcaggacgtc gagcgcgcca tcgacaccac gccagcgcgc tgcttccggg ctcgtgccct
      361 ccggccactg gaattcgtgc ggctgtagcg cgggcaggga ctggaagccc gagcgcgcgg
      421 tggcggcctt cagatgcaga cgcgagaatt ccccgagacg gcggaacaga gcttcgactt
      481 cgtagttgcc ggcgctgtgc atggagtgca tcaagtcctc gaagcgtcga gctgcgtcgc
      541 gctcgagctg cactgcgtgg gccaagaaga gctcgacgtt atccatagtg ttccattgga
      601 tcctgccgcg cactcgacgc ggcgacgcgg gccgcgaagc aatgcgcgtg ccacagccgt
      661 ttttgcgcta ttcttcgctt tgcactattc cgtggcgctg actttgtcgg gtttgttgag
      721 tctgtaacag aactaccagt cggcgacggc gagtcgctcc acggggctac cggcgatcag
      781 atgttgatcg acgatgggcg ctacgtcctc agcgccgacg tggccgtaga gaacaccctc
      841 cgggtacacg agcacactgg ggccgttttc gcaggggcct aggcaattcg taagcgttaa
      901 tcgatagtcg ttccacagcg cgcgccgcgt gaattcgtcc gaaaaggcct gatacaagct
      961 ggggctgccc ttagattggc aggagccgcg gggatgccct gcagggcggt tctgaacaca
     1021 aacaaagacg tgctttttgg tcagcattgc gagttcttcg tgggccagag aacacggcct
     1081 tacacgccct cccccgagaa acgcggccgc tctcgaactc gcagtaggca acaagcgtac
     1141 cgccggggca gcgtgctctc agaatcgacg aatcgggatt tggtacttct gcagtgcgta
     1201 cgcgatctgt cgaggggtga ggccaagcag acgagcggct ttggcttgaa cccagcctgc
     1261 ttgctccagt gccgcaatca aacgctcgcg cgaagcgtcc tcgccgagtc ttcgcagggg
     1321 cagctccccc gatgaagtcg agtggccccc ttggagccgc ggctcgcggt tagacggcgg
     1381 gggcggagac tgcgtttctc tcaaggcgag tgtcaggggc gtgtcctgct ccagccattc
     1441 gaaatcccgg gcggtcaggc gcgagccgga agccagcgat gctgcacggc gaacggcatt
     1501 ctcgagctct cgcacgtttc cgggaaagga atagtcctga agcgccgcca acgcgctcgg
     1561 gtcaaagacc aaacgcgtgc cgttgctctc gttgaaactg gccagaaact cggtcgctag
     1621 ctcggggatg tccgaggcgc gttcgcgcag agcaggtaag aacagaggaa caacgctgat
     1681 tcggtagtaa aggtcggctc ggaagcggcc ggtcctgacc tcctcttcta ggttgcggtt
     1741 ggtggcggcg agcacgcgca cgtcgacttt gagcgtgtgc atgcccccga cgcgctcgaa
     1801 ctcgccgagc tgcagcaccc gcagcagtcg agcctggaag gcgcccgaga tctcgccgat
     1861 ctcgtccaaa aatagtgtgc cgccgtcagc cagctcgaag cgccctttgc gctgggcgct
     1921 ggccccggtg aacgcacccc tttcgtgacc gaagagctcc gactccaata cactctcggc
     1981 cagggccgcg cagttcacgg agacgaaggg ccgttgaacc cgctgcgaca tgccgtgaat
     2041 atagcgcgcg agtacctctt tcccggtgcc ggactcacct cgcaataaaa cggtcgagga
     2101 gctcttggcc gcgatccggg cgcggtcgac ggtagctcgc caacgtggat ggccgcctga
     2161 catggtgctc gctccatgct tggcagcggg gctggtgcgc gggagctgag aagcggccgc
     2221 gtctgacgga gtgaggatcg gcgcgggctc ttcgactcct ttcgtttctg tttctttcga
     2281 ctcgaacgct ttggactgta ttgctctcgt ccctaaaagc tccgacgcgc ggctggcctg
     2341 cgcgatgcta tgcgcgatga ccgtgagcag tcgcacatcc gcgtcgaagc agaagcaagc
     2401 ctggtcaccc ggaccatgtg agcgctcgat ggcgagcacc ccaatgaacc gccccgcgtc
     2461 tttgatgggg acggcgacga gggacgcgtc gagtgcccga tctgtgtctg tttccagtgc
     2521 ctcctgc
//
LOCUS       DASTKU010000102         4312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_20312,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000102 DASTKU010000000
VERSION     DASTKU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20312"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            744..2066
                     /locus_tag="VFQ61_03660"
     CDS             744..2066
                     /locus_tag="VFQ61_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953572.1"
                     /translation="MASLRLAVCIVLGATVIAACSSDGANSGAQPTTVADITPITRVE
                     DAKPGVFIPPFVDCRPPLPGEKTEAPSGKVCTPVSIAGATEPGKEFANYAACDIVRTQ
                     RPYYPSAPAAVPDPQDPRLDDPTFKTEVEWARVQVAATGCVCCHDSRYAPRGPSQWDI
                     SAAGVWLDTVSDSCLALFAGLADSSVLGAFPVSENNGFDRMQVGVPSTDPARMRALVE
                     QELMRRGVSEEDSRKVPPFGGPIYESSVRPPTSCGAGEGVAPDGHVFFKSSARYVYVL
                     ENGSNNPGVPPNLDRPAGTLFRLDVLASQDPIESGTLTYGSTPQGSFQVVPAEGTAPA
                     LQTGKVYQLTALRDVGVPVTNCTFVFGEPVPTADPEPMPSDSFGAKCGKPEDCSAPAV
                     YCAVQPGQTEGYCTASGCKEDASRCPGGWGCFDTSMFQPGGPSFCSKP"
     gene            2118..2912
                     /locus_tag="VFQ61_03665"
     CDS             2118..2912
                     /locus_tag="VFQ61_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953573.1"
                     /translation="MKHWASDMGPSGWGLMTLLVAGALGSGCRERGAPRPLRASMPSA
                     APTVSAVSAADPCRKTALCQQFGRCSYRAVGASGLGSDWVVIEPQAKPADREYFSCVA
                     LLGSDCEASRIACQEHGECAAQSGRCVAVSDARCVASEACKRTGLCTAVAGWCQAGKS
                     GSCERSQACRSNGACREVTVSVERIPDIYFASRKSLLSAFDATTGDLWAEPIPLVEPR
                     SFAVENETAKILSCAALDSESCRASDECRTQGRCMALDGECQVKRP"
     gene            complement(3233..3475)
                     /locus_tag="VFQ61_03670"
     CDS             complement(3233..3475)
                     /locus_tag="VFQ61_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953574.1"
                     /translation="MTLVYVFVALTLVAVAAVMITSRLFPDPPNLTDEDVVAELRKGH
                     PITAIRWYRAIHGVGLKKAKSAVAELAKDHGIATKR"
     gene            complement(3826..3984)
                     /locus_tag="VFQ61_03675"
     CDS             complement(3826..3984)
                     /locus_tag="VFQ61_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953575.1"
                     /translation="MGSPGVALAAARIAAERVRLERLGIIDATGELISKELPPDMVAE
                     SDTTLETG"
BASE COUNT          735 a         1374 c         1380 g          823 t
ORIGIN      
        1 cgctgccctc tgttggccgg catctggcgc gcacacttag cctgcgacaa ctgccacgac
       61 cgcgccgacg tcgctggctt gcaagtcggg ggggcgagcg ctcttgtaca gtgttcggca
      121 ataccctgac ccattcacga gtgacgatgc cgtgcatctg cacagaccgt tgaaaacggc
      181 acgtgctcgt ttgcggaaac gcggactgtg tcgagcgcgg cagtctgggg ttggctaccg
      241 gcgatggcgc catcgtccat cctctgcgcg aggcttgctc ggacaattcg acgcgtgcga
      301 tcgagtcgtt caccggatcc tttcatcgcg gcacgcgggc gtaagggcgg cagcaccagc
      361 accgtcgcca tcttgcacgc gcagctctag ttcatcggag gattcgacac cggtgaggcg
      421 atgagcccgc gcaaggcggt ggtcgtgatc ggctcgggca caaccacggg cgtgagcacg
      481 cgccgtgcgg ctcggtgacg aaacggcttt cgcgactcga tccccgatcc acgctcgatg
      541 gaccgcgcgg caggatcgag gtcgagtcgg gaaaattgcg gcgcgagtgc gcatacagcg
      601 cagcgttggg cgggtcctat ctgctcaccg aggaatgctg accaggttag acacagtgta
      661 cagctggccg atacgcttgt tgccaaacat gacagcgggg tctgccgatt gacactgcgg
      721 agtcgctgcc caagttcctc gggatggcgt ctttacgtct cgccgtatgc atcgttcttg
      781 gtgcgacggt catcgcagct tgttcgtccg atggggcgaa ctcgggggct cagccgacga
      841 cagtcgctga cataacgccc atcactcgcg tcgaagacgc gaagcccggc gtcttcatcc
      901 cgccgttcgt cgactgccgt ccgcctctgc ccggcgagaa gaccgaggcc cccagcggta
      961 aggtctgcac cccggtttcc atcgctgggg cgacggaacc aggtaaagag ttcgcgaact
     1021 atgctgcgtg cgacatcgta cgcacgcagc gcccgtatta tccgagcgcg cccgcggctg
     1081 tcccggatcc acaggatccg aggctcgacg atcccacgtt caagaccgaa gtcgagtggg
     1141 ctcgagtgca ggtcgcagcc acgggttgcg tgtgctgtca cgactcacgg tacgcgccgc
     1201 gaggcccttc gcagtgggat atcagcgccg ccggcgtctg gctcgacacg gtttcagaca
     1261 gttgccttgc gctgttcgcc ggccttgcag actcctctgt tctcggcgcc ttcccggtct
     1321 cagagaacaa tggctttgac cgcatgcagg tcggagtgcc gagcactgac cctgcgcgaa
     1381 tgcgcgcgct cgtcgagcag gagcttatgc gtcgaggcgt cagcgaggag gattcgcgca
     1441 aggtgcctcc gttcgggggt cccatttacg agagcagcgt gcgtccgcca accagctgcg
     1501 gagcaggcga aggggtcgcg ccggacggcc acgtgttctt caagagcagc gcgcggtacg
     1561 tctacgttct ggaaaacggc tcgaataacc ctggggttcc gccgaacttg gaccgacctg
     1621 ccgggacgtt gtttcggctc gacgtgctcg ccagccaaga cccaatcgag agcggcaccc
     1681 tgacgtacgg gtcgactcca cagggcagct tccaagtcgt cccagcggag ggcaccgcgc
     1741 ctgctctgca gaccgggaaa gtgtaccaac tcacggcgct ccgcgatgtc ggggtcccgg
     1801 tcacgaattg cacgttcgtt tttggggagc cagtgccgac ggcagacccc gagccgatgc
     1861 ccagcgattc attcggggcc aagtgtggaa aaccggaaga ctgctctgcc cccgctgttt
     1921 actgcgcggt ccagccagga caaaccgagg gttattgtac ggcaagtgga tgcaaggaag
     1981 acgcgagccg gtgccccgga ggctggggtt gtttcgacac gtcgatgttt cagcctggcg
     2041 ggccatcgtt ctgctcgaag ccgtgacgcg ccacattacc cgcagcgcct ctctacccag
     2101 cggcggtgaa gcaggtcgtg aagcattggg cgagtgacat ggggccttct ggttggggct
     2161 tgatgacgct gttggttgcg ggtgctctcg gcagcggttg tcgagagcgc ggggcgcctc
     2221 gtcccctccg cgccagtatg ccgagtgccg cgccgacggt atcggcggta tccgcggccg
     2281 acccatgcag aaagaccgcc ttgtgccagc aatttggccg ctgcagctat cgcgcggtgg
     2341 gggcgtcagg gcttggtagc gattgggttg tcatcgagcc gcaggcgaaa ccggcagatc
     2401 gcgaatactt cagttgcgtc gcgcttttgg gctctgactg cgaagcttcg cgcattgcct
     2461 gccaagaaca cggcgagtgc gccgcacagt cggggcgttg cgtcgccgtg agtgatgcgc
     2521 gctgcgttgc gagcgaggca tgcaagcgca ccgggctctg cacggccgtg gccggatggt
     2581 gccaagccgg caaatcgggc tcctgtgagc ggtcccaggc ttgccgaagc aacggggcat
     2641 gccgcgaagt caccgtttcc gtggagcgaa taccggacat ctacttcgcg tctcgcaagt
     2701 cgctgctttc ggccttcgac gccaccacgg gcgacctttg ggccgagccg atcccgctcg
     2761 tcgagccgcg ctcctttgcc gtcgaaaacg aaacggccaa gatcctaagc tgcgccgcgc
     2821 ttgattcgga gagctgtcga gcgtccgacg agtgccgcac gcaagggcgg tgtatggcgc
     2881 tagacggaga gtgccaagtc aagcgaccgt agccacccag cggcttggct cggcgcgcat
     2941 agccagtcga ggcgctagtt cgtgtcgcct tgtggcccag gtgtcacgac gagttcgagt
     3001 cgagccggga cgacttcggt gagagctagc tcgactcctc tcgccgccaa ttccaaccga
     3061 gcgggatcga atcccccgca gccccttttg ctggcgagcg gcggtagcgt accgcgaacg
     3121 tggggtgcaa ccgtcgcgcg ttcctgaagg cctgacgcct gcgcccaaac gtgaaacgtt
     3181 agccacccga cacgggtcct cgctcgagcg gacggttcgc cattccgaac tcctatcgtt
     3241 tggttgcaat gccgtgatct ttcgcgagct ctgctactgc cgattttgcc ttcttgagcc
     3301 ccaccccatg aatcgcccga taccaacgga ttgcagtgat tgggtggcct tttctgagct
     3361 ccgcgacaac atcttcatcg gtgagattgg gcgggtccgg aaagagccgg ctggtgatca
     3421 tcacagccgc cactgcgacc agtgtaaggg ccacgaaaac gtaaacgagc gtcattcacg
     3481 gcctccgatc gcgcaccttt agttgccacg gtcatacgct ggcgccacgt tgtctcttcc
     3541 gccgaattcc ccccgcggac cagctgttct ttcgttattc gcaacggctt gctcagcaag
     3601 cgggtttgcg gtgcgcacct ctgccttgag gcttttcgct gccgtcgtga acaccgtccg
     3661 tgcattgaac gtcgcgcgtc cattggcacg gtagcttccg agaatctgcg cgcagcggtc
     3721 atcgatgttg aacccatcta cgctgaatgc gctaaccgga aagcttctcg tcttaccgct
     3781 gcctgggccc ggcgacgacg atcatgcgcg gacgcgacat gtatatcagc ccgtctcgag
     3841 cgtcgtgtcc gactcggcca ccatatcggg tgggagctcc ttcgagatga gctccccggt
     3901 cgcatcgatg atccccaacc gttcgaggcg cacgcgttca gccgcgatgc gcgcagccgc
     3961 gagcgccaca cccggcgatc ccactccgcc tcgtcctcag gcttgtttca ggcggcatat
     4021 cgatgcggtc gccctgattg cctctgcaga acgggagcgc gcgattttgc ggacggcgcc
     4081 caacgggcct taacgtgcac cgccaggaag cacgcgcggc gtctcgtcga cggcaaggtg
     4141 ttcctagctt gcgcacatcg cgcgccttac actccgcacg cgcaaagccg gcactctgac
     4201 ctcgccatcg ccgcgttcgt tcacgcgaca ggatgttacg cgagttcccc tcggcttcgt
     4261 cggaatagcc gtgaggcttc aggtacttgt gttgtggagc cgagggggat cg
//
LOCUS       DASTKU010000103         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_466644, whole genome shotgun sequence.
ACCESSION   DASTKU010000103 DASTKU010000000
VERSION     DASTKU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466644"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..717)
                     /locus_tag="VFQ61_03680"
     CDS             complement(49..717)
                     /locus_tag="VFQ61_03680"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9953576.1"
                     /translation="MTEDREDLALITAKTLKHYNERAAEFWEGTRDHDVKQNVEALLR
                     HIRGSPPLRILDFGCGPGRDLVTFRALGHDPIGLEGSPPLAAMAREHSGCEVWEQDFL
                     ALRLPAGRFDGIFANASLFHVPCKELPRVLSELHAALKPDGVLFTSNPRGNNEEGWNR
                     GRYGAYHDLESWRAFLERAAFTELEYYYRPPGLPLERQTWLASVWRRNGAASAACPAA
                     QPGR"
     gene            complement(1055..1258)
                     /locus_tag="VFQ61_03685"
     CDS             complement(1055..1258)
                     /locus_tag="VFQ61_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET9953577.1"
                     /translation="MNFVIECEREDDGRWLAEVPQLPGVLAYGDSANDAMAKAQVLAL
                     RVLAERLENHETGPQAITFSIAA"
     gene            complement(1404..2246)
                     /locus_tag="VFQ61_03690"
                     /pseudo
     CDS             complement(1404..2246)
                     /locus_tag="VFQ61_03690"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2210..2587)
                     /locus_tag="VFQ61_03695"
     CDS             complement(2210..2587)
                     /locus_tag="VFQ61_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9953578.1"
                     /translation="MPARKGPKKVNEYSLELKRTAVRLTQAPGARVKDVAESLDIHPF
                     MLSRWRKLAREGLLSEKRKATPSGKKKRAPSMVEMSKYAKLKRELQALRKEHEFLKKF
                     DRFRAQLKAKGSRSSGGSGSGSR"
BASE COUNT          460 a          855 c          815 g          520 t
ORIGIN      
        1 gtgcgccagc cgccgcgcgg aagcgccggg cacgggttcc gacgagctct aacgaccggg
       61 ttgagcggcc gggcacgctg ccgatgcggc gccgttcctc cgccaaacgc ttgcaagcca
      121 ggtctgccgc tcaagcggta accccggtgg acgatagtag tactccaact ccgtgaacgc
      181 cgcgcgttcg agaaacgcgc gccatgattc gaggtcgtga tacgccccat accgtccgcg
      241 attccagccc tcctcgttat tcccccgggg attcgaagtg aagagcacgc catcgggctt
      301 gagcgcggca tgcaattcgc tcagtacgcg cggcagctcc ttgcatggca cgtggaacag
      361 cgaggcgttg gcgaagatgc cgtcgaagcg gcccgcggga agcctcaggg cgaggaaatc
      421 ctgctcccag acctcgcagc cgctgtgctc gcgtgccatg gcggcaagcg gcggagagcc
      481 ttcgaggccg atcgggtcgt ggcctagcgc acgaaacgtc accaagtcac gccccgggcc
      541 gcagccgaaa tcgagtatgc gcagcggtgg cgaaccgcga atgtgccgca gcagcgcctc
      601 gacgttctgc ttcacatcgt ggtcgcgcgt gccctcccag aattcggcgg cgcgttcgtt
      661 gtagtgtttg agcgtcttcg ccgtgatgag agccaagtcc tcgcgatcct ctgtcatcgg
      721 gatccttcga tttcctgtga gatctagcga ccgaattgag cggccgggca caacgtcgat
      781 gccgcggcca gacgcggccc accgtgcctc acggtccgct ccaacgcggt gttaggcatc
      841 tcgtgcctac aggtcttccg gcgtgatgcc cgtgtgcttc gcgatccgag ccagcatctt
      901 ggtccaattt cctctccgtc atgaaatgcg aagacgaaat ccgggtagcc gttgcggcta
      961 agcgtgcgat gtgaccccga ttgacgcttg agttgccacc cgacgctcag caacgccgcg
     1021 aaaacgcgac gcgccttggt cgaaggccaa tgtgtcacgc agcaatgctg aaggtgatgg
     1081 cctggggtcc ggtctcgtga ttttcgagac gttcagccag cacgcgaagg gcgaggactt
     1141 gtgctttcgc catggcgtcg tttgcagagt caccgtacgc aagaacaccg ggcagctgcg
     1201 gtacttccgc cagccaccgg ccgtcgtcct cgcgctcgca ctcgatcaca aaattcatgg
     1261 cggtgcctat tttacgccgc tgcttgcgag atgcctaacg accgagttga gcggccggcc
     1321 acaatgccga tgccgcggcc agacgcggcc caccatgcct cacggggatc ttgacccgat
     1381 tcagtggaca catgggcgca gtttcaagcg ttagttcgct cgtagtcaat gggcgagcgg
     1441 tagccgaggg ctgagtgtgg gcgacgtcga ttgtagtgcg cgatgtagcg gcgaacggcc
     1501 cgatcgaggt ctccacacgt ggtgaatcga tagccatgga tcgcctccgt cttgagcgtg
     1561 tggaagaagg actcgacgtg ggcattgtcg gtcatggttt ttggacggtt catgctttgc
     1621 acgatgccgt ggaccgcgag cccatcgcga tagaggtagc ctccatattc acttccgcgg
     1681 tcggagtgga agacgagacc ctcgccaggg tgccggcgcg ccgtcgcctt gcgcaacgcc
     1741 gcgagcgtga gatccgcggt gcgctgattg ccgagctcgt agcccaatag ccgacgggag
     1801 tactgatcga tcaccacggc gaggtagcgc cacgctccgc cggccttcaa gtacgtgatg
     1861 tctccacgcc aaacttggtc cggcgcactc acgatcctct cgcgtgcctc gttcggactg
     1921 cccaggcgac acgtgaccga accgggcacg ttcttgtaga tgcggttggc gcgcgccttg
     1981 aggttcagct cccgcataag ccgcgcgacg cgttttcgtc ccgtgcgctc gccttgccgc
     2041 cgcaggaggg cgtgaatccg cggactgccg taaacgccgt aactttgctc gaagtgctcc
     2101 tcgatctgcc tgcgcagccg cacgtcacgc cgcgcgtgat cactcgcggg ccgcttgagc
     2161 caggcgtaaa agccggatcg tgagacctcg agcctttcgc acatccatct caccgggaac
     2221 cgctcccgct cccgcccgat gaacgcgaac ctttcgcctt cagttgcgca cggaagcgat
     2281 cgaacttttt taggaactcg tgctcctttc ggagcgcttg gagctcccgc ttgagcttcg
     2341 cgtacttcga catctccacc atcgacggag cgcgcttctt cttgcccgag ggcgtggctt
     2401 tccgcttctc gctcaagagc ccctctcgcg caagcttgcg ccaccgcgac agcatgaatg
     2461 gatgaatgtc gagggattcc gcgacatcct ttacgcgcgc ccctggggcc tgcgtcaaac
     2521 gcacagcagt gcgcttgagc tccagggaat actcattgac cttcttgggt ccctttcggg
     2581 ctggcattga acacctccgg ttgttagccc aaggtgtcta ctttatcggg tcaggattcc
     2641 ggtccgctcc
//
LOCUS       DASTKU010000104        14781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_911972, whole genome shotgun sequence.
ACCESSION   DASTKU010000104 DASTKU010000000
VERSION     DASTKU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14781
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911972"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2918)
                     /locus_tag="VFQ61_03700"
     CDS             complement(<1..2918)
                     /locus_tag="VFQ61_03700"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9953579.1"
                     /translation="MDQVLSEFHPALSTWFTRRFVAGPTRPQAQGWPVIRRGHDTLIA
                     APTGSGKTLSAFLVCIDRLYREAQTRVAPGAGVRIVYVSPLKALTVDIEHNLGRPLNE
                     IASVARELGFEVPVLRTRVRSGDSAAKDRAAIVREPPEFLITTPESLYLMLTAERSRG
                     LFASVESVIVDEIHALARDKRGSHLALSLERLDRLCRKRPQRIGLSATQRPLHKVAEL
                     LVGYEAGRPRACEIVDTGHQRELELTLMLPDRELEAVASGAHMQEVVSKLCEAIEQRR
                     TTLVFVNTRRLAERLAHNLAERLGTDSVAAHHGSLSKERRQRVEQRLREGSLRALVAT
                     ASLELGIDVGDVELVCQVGSPRSIATFLQRVGRSGHTRFGKPVGQLYPLTRDELLECT
                     ALLRAVSHGELDCLTIPEAPLDILAQQVVAECAAAEHTERELFDWIRRASPFARVSET
                     DFERVLQLMSEGIDTGRGRRAAFLHRDRVQGRVSARRGARLAALTSGGAIPENADYRV
                     IADPEETFVGTINEDFALESAAGDVFLLGSTSWRVRRVERGVVRVTDAQGSAPTLPFW
                     LGEAPGRTHELSQQVSSLRRMAQQALVRGGEEALQAELAAWPGLAGAACGELTRYLGA
                     GYRALGVLPNDRDLVFERFFDDTGGMQVVVHAPLGARINRALGLLLRKRFCRSFDFEL
                     QAAASDDAIVLSLGPQHSFPLEDMQDYLQPRGVREALTKAVLQIPMFQVRWRWNLNRA
                     LVVLRFRGGRRVAPMIQRMESDDITAAVFPALSACQDNGPIALEPPDHVLVQQTLHDC
                     LTEALDIDGLEQVLAQISAGEVRVHFRDTTEPSPLSHEILSARPYTFLDDAPLEERRT
                     RAVSVPRQLNGDARGPGELDPAAIARVEAEVRPDPRTADELHDLLCGLGVTRPLEAFQ
                     SLFEELVRAGRALHVGFDAQMLWGATESREGLEALFPHASFEPDYRLTPASRQDPQPA
                     V"
     gene            complement(3003..3782)
                     /locus_tag="VFQ61_03705"
     CDS             complement(3003..3782)
                     /locus_tag="VFQ61_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9953580.1"
                     /translation="MTDGIRWEKRGSAAIVVLDRPDRMNALTRASVAALGQVGRELAQ
                     DPELRAVILTGAGSRAFCAGADLRERASMSESEILAMLESYRTELRWLGESSRPTVAA
                     INGVALGGGLELALACDFRIANEDAVLGLPETSLGIIPGAGGTQRLPRLIGPARAREM
                     ILLGRRLTAPEALAWGLVHRVNLAPLDLIEAALEFIKPILDGAPLAQAAALRALRQTD
                     SELETGLEVELDAYRTCLASEDRKEALRAFQEKRKPIFRGC"
     gene            complement(3779..6388)
                     /locus_tag="VFQ61_03710"
     CDS             complement(3779..6388)
                     /locus_tag="VFQ61_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HET9953581.1"
                     /translation="MSRFERLHERCACSALGPSPFSLAGTERKYERSRPFTVRHTFLD
                     IELDFARRSVSGRAFLEIERMAPDAVNLDLDAIGFEIASVALAEAADQRGLPFEYDGE
                     RLRVSGFEGRDRARIEIVYRAVPQRGLYFLGPDAQVKDRPVQVWTQCQDEDARHWFPC
                     HDKPHVKMTSELRVKVPAEFVALSNGDLLSEKRAKNGVGTFHFKLDSPHPSYLVTLVV
                     GRFAIEKDRDAELEDGRRIPVRYYVPVSRAKDAKRSLGETPRMIELFSRLTQVPFPFS
                     RYSQVVVHDFVFGGMENTTATTLYEHVLLDARATLDVTSNDLVAHELAHQWFGDFVTC
                     RDWSHAWLNEGFATYFEHVEREHRLGRDEYDYGVRGDVNSYLSEAGGRYRRPIVCRDY
                     SQPIDLFDRHLYEKGGLVLHMLRRELGDQLFFAGVTSYLREHANGIVETNDLMRAFER
                     VSGRSLEQFFDHWVYRAGHPDLKVKVSYEDGLVSVALKQSQKPQENAIFEFDFEIEIL
                     DSAGERTRHKKRCSGAEDSLTVPLPRRPQYVAFDPEYRVIGSIGFEAPSDMLRNQLAH
                     GSSALLKWVAAEALARKPDQTALGALGQCLSTESQPWMVRVEAARALGSTRSPLALEE
                     LAKHVKTKHPKVRRAVAGALGQFRNERAFDLLRPLAAKDPSYLVESEAARSLGRTRQK
                     GALKLLTSLLSRDSWADIVRMGALDGMGALRDEAALDLVIEHTRYGHPTRGRRAALSA
                     LPDLSESKRVREHLESLLDDPDPHLRSDVASALVALRDARSRPALRRALEHELDGRVV
                     RRLREALRDLSDTSGDRKRLADELETLRNELTELKARLARLESKKPEAETPPKPRRAT
                     LPALNKNRKGRRA"
     gene            6526..8706
                     /locus_tag="VFQ61_03715"
     CDS             6526..8706
                     /locus_tag="VFQ61_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET9953582.1"
                     /translation="MPVTLSGEVERVTFENETTSFRVLRVGSLEGDAGRRGSVVVVGT
                     FQAVGPGTRVRITGEFVQDARHGEQLRADVLVPLEPNTLVGLERYLGSGLIPGIGPSL
                     AKRIVDQFGLDTLKVLDEAPERLAGVPGLGKRRVNDIKENWALQRALSSVLVLLRTHG
                     ASHNLALRIWKHYGDRAASIVQRSPYRLALDVRGVGFKTADRLAQSIGIVSDHPERCQ
                     AGVLHVMEEISEAGHIHTPRADVVAHTAELLRVDAAHIEAAVDVLYAAGRLRVEQGSL
                     FLEQFYSAEVETAQHILSRTGHRQATQMPAGNVERAIAAFEAHSKISLASTQRQAVEL
                     AARERIAIVTGGPGVGKTTIVRAMLRVIESQRLKVALAAPTGRAAKRLSEATGHPAST
                     LHRLLEFDPRSGCFQRNLASPLEAHALIVDEASMIDLEMMQALLGALSPSAKLVIVGD
                     ADQLPSVGPGAVLRDLIESRVVGTVRLNEIFRQADTSGIVENAHRILAGEEPKTAAPE
                     DPRGDFFVIKRRTPEDAAQVIRELVTERIPKRFGLDARRDIQILSPMHRGPAGTLALN
                     EMLQSALNPDGSAIEVRGQKLRQGDKVIQTRNDYDRDVFNGDVGSVLQVDTKERKLVV
                     RFDQGDIEYDDSNLASLNLAYALSVHKSQGSEYPAVVIPLLTTHFMMLNRSLLYTAVT
                     RARKLCVLVADPRALSLALADTRREERRTRLAARLQGDAGAGKL"
     gene            complement(8818..9144)
                     /locus_tag="VFQ61_03720"
     CDS             complement(8818..9144)
                     /locus_tag="VFQ61_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET9953583.1"
                     /translation="MGNRLFVGNLSFSTSQASIESAFQAAGEVREVALPLDRSTGQPR
                     GFAFVTMANSHAAAAAIEQLNGAILDGRQIRVDEAQERTGGGGGGGGFRGGGGGGGSR
                     GGRNRY"
     gene            complement(9690..9935)
                     /locus_tag="VFQ61_03725"
     CDS             complement(9690..9935)
                     /locus_tag="VFQ61_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953584.1"
                     /translation="MDRRVKAVKKDRTGNIVALCNAGENWSPRRKKDVIRDIDRNRSS
                     YYVEEIGRRAYLRVVNGCLQTSTAEDNQNRLDRLPAI"
     gene            10852..11190
                     /locus_tag="VFQ61_03730"
     CDS             10852..11190
                     /locus_tag="VFQ61_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953585.1"
                     /translation="MNSSKSPARGKESKPECLAIAVQYRNKRGMVYELNSVDGSVDIH
                     VLEKSHSEPQVHIEAHHGRLADAVVISKNGNNCRDVLREIGAEWETRRSLPHVNWDTV
                     AALLSSVRAI"
     gene            complement(11377..12093)
                     /locus_tag="VFQ61_03735"
     CDS             complement(11377..12093)
                     /locus_tag="VFQ61_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953586.1"
                     /translation="MQRMLFSILGVMLLNACASAAPDGDIEAQSGISQGLVTSSLDLD
                     SSPSLAVHAHSICTDWFGPQVHGKCPRAPGWKTYPVLDFCEYVWRRSSPPDSRPEVVN
                     ARQLEPALASCTSTSSYALVVEASSVPVRDQFPEAVSLDPAPGQAAPPAPQTELADPG
                     SDAEDGPETQYAISNVSNGKDGGRPIYYAAVRKLWAKQQFLFKLNGIQYQVPSQGLFD
                     LVGAPTTVTSVEPAWTVVAQ"
     gene            12222..12938
                     /locus_tag="VFQ61_03740"
     CDS             12222..12938
                     /locus_tag="VFQ61_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953587.1"
                     /translation="MNLRSCSPARDHDTLLFVMVDEDVALVRRVLGGEAEAHAEFVRR
                     LSHVVRHRVARVLMGAGAKPGSTRSRQALLDLMQEVYVVLLDRKGRVLSTWDPARGLS
                     LENFVGLVAEREAIAFQRSGRRSAWAEQPTDDLVDHTLLRTSPEDEAAAKEQLELLLE
                     HLSERLSPQGLLLFEALFVSETPVPEICTLFATTPTAVYNFRARLKQVVSDWKRRSER
                     THGASTRNVVDGGRVLRSEP"
     gene            12935..13705
                     /locus_tag="VFQ61_03745"
     CDS             12935..13705
                     /locus_tag="VFQ61_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953588.1"
                     /translation="MSKKSGADAEGVLRQLAELHTVRTARLRDCDPDADGADAAFARL
                     TDEEESRLVEAVVRRTRETPARRPLAGAVANPDQGLRRFGFERGRRYAGAWAAAATLI
                     LAGGAVYGTRAAPSWPEYVLASPPSDAQFRAAVDASVTAESDAVDHVYGIGRELRFVV
                     RPRHPFAEAPSVRVYAGSNGTEALRAVRLEPLEGGSIRVSLKTGPGGYVPRPGPDQLL
                     IVLGERSAWRSLVRRLSHPAGDVVQSFRVPVTWHRVNE"
     gene            13749..>14781
                     /locus_tag="VFQ61_03750"
     CDS             13749..>14781
                     /locus_tag="VFQ61_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953589.1"
                     /translation="MATAFLALFSAQASTGQSTSAATVQDDSVAAVSGCAAVVGTRIC
                     ELSAGSERELRVWLPQLSDEYARLLVQLDGRKVSAFRVTPSEHGYRLTIPIELTRDRA
                     QLSVFIDHRSILQLDLRRAGEWPWQREPAGALRPATAALRDRLPLARGAERSKLLRAL
                     AQTEVAAGRLEPALRLFEEGARGAEEQQRHSDARGHWLQRAYHQAHAHDFGAARASVA
                     HADQVSSAAFVDADGRAAASYVRGVLAAQMRDLRGAQAAYEEAQRWVNRGATLMRPQL
                     AVAQAFVAEQQGRLGDAVAILERELEADLAKSSPCFHAIVLTNLGWYELLRGLTEGRS
                     SVSAPRAQQR"
BASE COUNT         2682 a         4749 c         4693 g         2657 t
ORIGIN      
        1 acggcgggtt gcgggtcttg ccgagaagcc ggggtcaggc ggtagtcggg ttcgaaagat
       61 gcgtgcggga acaaggcctc cagaccttca cgcgactcgg tcgcacccca cagcatctgc
      121 gcgtcgaacc cgacgtgcaa cgcgcgcccc gcccttacta gctcttcgaa cagactctga
      181 aatgcctcga gcggacgggt gaccccgagc ccgcacaaca aatcgtgcag ctcgtcggcg
      241 gtgcgaggat cgggccgcac ctctgcttcg acgcgcgcta tcgcagcggg gtcgagttct
      301 cccggaccgc gagcgtcccc gttcaactgg cgaggcacgg acacggcgcg tgtgcggcgc
      361 tcctcgagcg gcgcgtcatc caggaacgta taaggccgcg cgctcaagat ctcgtgcgag
      421 agcggggagg gttcggtcgt gtcacgaaag tgcacacgca cctctcccgc ggagatctgc
      481 gcgagtacct gctccagtcc gtctatatcc agcgcctccg tcaagcagtc gtgcaacgtc
      541 tgctgtacga gcacatgatc gggtggttcg agggcgattg ggccgttgtc ttggcaggcg
      601 gacaaggcgg gaaacaccgc cgccgtgatg tcgtcggatt ccattcgctg gatcataggg
      661 gccacgcgtc gcccaccgcg gaagcgcagc accacgagcg ctcgattcag gttccagcgc
      721 cagcgcacct gaaacatggg tatttgcagc acggccttgg tgagtgcctc gcgaactcct
      781 cgcggttgca gatagtcctg catgtcttcg agtggaaagc tgtgctgtgg tccgagagac
      841 agcacgatgg cgtcatcact ggccgcggcc tgcaactcga aatcgaaaga gcgacagaag
      901 cgtttgcgca gcaataagcc cagcgcgcgg ttgatccgtg ctcccagtgg cgcgtgcacc
      961 accacttgca tgccgcccgt atcatcgaag aagcgctcga acacgagatc tcgatcgttc
     1021 ggtaacacgc caagcgcgcg ataccccgcc ccgagatagc gggtgagctc gccgcacgcc
     1081 gccccggcca aacctggcca cgccgcgagc tcggcttgca gcgcttcttc tccgccccga
     1141 accaaagctt gctgggccat gcgccgcaaa ctcgagactt gttgggatag ctcgtgggtg
     1201 cgcccgggcg cctctccgag ccagaacgga agcgtcggcg cgctaccttg cgcgtcggtg
     1261 acgcgtacta cgccgcgttc cacgcggcgc actcgccaag aggtactccc gagcaagaac
     1321 acgtctccgg cggcgctctc gagtgcgaag tcctcgttga tggtgcccac gaaggtttct
     1381 tcggggtcgg cgatcacgcg atagtcggcg ttctcgggga tggcgccgcc cgaggtgagt
     1441 gcggccagcc gcgctccgcg ccgcgcgctg acacgcccct gcacccgatc ccgatgtaga
     1501 aaggcggcgc gccggcctcg ccccgtgtcg atgccttcgg acatcaattg cagcactcgt
     1561 tcgaagtccg tttcgctcac gcgtgcgaac gggctcgcgc gtcgaatcca gtcgaagagc
     1621 tctcgctcgg tatgctcggc ggcggcgcac tccgccacca cctgttgcgc cagaatgtcc
     1681 agcggcgcct ccggaatcgt cagacaatcg agctcaccgt ggctgaccgc gcgcagcagc
     1741 gcggtgcatt ccaagagctc gtcacgcgtg agggggtaaa gctgccccac gggtttgcca
     1801 aagcgcgtat gccccgagcg gcctactcgc tgcaggaagg tcgcgatgct gcgcggcgag
     1861 ccgacttggc aaacgagctc gacgtccccc acgtcaatgc ccaattcgag cgatgcagtg
     1921 gcgaccagcg cacgcaagct gccctcgcga aggcgttgct ccacgcgctg ccgacgctcc
     1981 ttggacaagc tgccgtggtg cgcggcgaca ctgtcggtcc ctagacgctc agccaagttg
     2041 tgggcgagcc gctcggcgag ccggcgcgta ttgacgaaca ccagcgtggt ccggcgctgc
     2101 tcgatcgctt cacacaattt gctgaccacc tcctgcatgt gggcgccaga agccacggct
     2161 tcgagctcgc gatccggtaa catcaacgtt agctcgagct cgcgttgatg acccgtatcg
     2221 acgatctcgc acgcgcgcgg ccgcccggct tcatagccca ccaagagctc ggccaccttg
     2281 tgcagtggcc gttgcgtggc ggacagcccg atgcgctgcg gtcgcttgcg acacaaccga
     2341 tcgagtcgct ccagggagag cgccaagtgc gagcctcgct tgtcgcgggc cagcgcgtgg
     2401 atctcgtcca cgatcacgct ctccacgctg gcgaacaggc cccggctgcg ctccgcggtc
     2461 aacatcaagt aaagcgactc gggggtcgta atcaggaact ccggcggctc gcgcacgatt
     2521 gccgcgcggt ctttggccgc gctatcgccg ctgcgaaccc gcgtacgaag caccggtacc
     2581 tcgaagccca gctcgcgtgc gacgcttgcg atttcattca gaggccggcc caggttgtgc
     2641 tcgatgtcga ccgtcaaggc cttcaacggc gacacgtaca cgatgcgcac gcccgcgccc
     2701 ggagcaacgc gcgtctgggc ctcgcgatac aagcgatcga tacacaccaa gaacgcagac
     2761 agcgttttgc cggacccggt gggcgcggcg atcaacgtgt cgtgtccgcg tcgaatcaca
     2821 ggccagcctt gcgcctgggg gcgggtcggc cccgccacga atcggcgcgt aaaccaggta
     2881 ctgagggcgg gatggaactc ggaaaggacc tggtccatgg cgcgcccaga gcgtagctac
     2941 gtgtctcgga ttagccagct cgaggctcga acggtgcccc gagcgcccgt caatccgcgc
     3001 ggttagcaac cacgaaagat cggtttgcgt ttctcttgga acgcgcgcag cgcttctttg
     3061 cgatcctcgg acgcaaggca agtccggtaa gcatcgagct ccacctcaag ccctgtctcc
     3121 agttccgagt ccgtttgacg caaggcgcgt agggccgcgg cttgggccag cggcgcacca
     3181 tctaagatgg gcttgatgaa ctccagcgcg gcctcgatca aatcgagtgg cgcgaggttc
     3241 acccggtgca cgaggcccca agcgagcgcc tcgggagcgg tgagtcgccg cccgagcaag
     3301 atcatctctc tcgcgcgggc cggcccaatc agccgtggca agcgttgcgt tccgccggct
     3361 ccggggatga tgcccagcga agtctcgggc aagccgagca ctgcgtcttc gttggcaatc
     3421 cgaaaatcgc aggccaacgc gagctcgaga ccgccgccca aggcgacgcc attgatcgcg
     3481 gccaccgtcg gtcgggagga ctcgccgagc cagcgcagct cggtgcgata ggactccagc
     3541 atggccagga tttcgctctc gctcatgctg gcgcgttctc gcagatccgc tcctgcgcaa
     3601 aacgcccgtg atcccgcgcc ggtcaagatt accgcgcgca gctccggatc ctgcgcgagc
     3661 tctcgcccga cttgccccaa ggccgcgacg ctcgcgcgcg tgagcgcgtt catgcgatcg
     3721 ggccgatcca gcacgacgat cgcggcgctc ccgcgcttct cccagcggat cccgtcagtc
     3781 atgcacgcct tcctttgcgg ttcttgttca gcgccggcaa agtggcccgg cggggcttgg
     3841 gtggggtttc ggcctcgggc ttcttgctct ccaggcgcgc caaacgcgcc ttcagctccg
     3901 tgagctcgtt gcgcagcgtt tcgagttcgt cggccagacg cttccggtcg ccactcgtgt
     3961 cactcagatc gcgcagcgcc tcccgcaggc gccgcaccac acggccatcc agctcgtgct
     4021 ccagggctcg acgcagcgcc ggccggctac gcgcatcccg cagcgccacc agcgcgctgg
     4081 ctacgtccga gcgcaagtgc ggatcgggat catccaacaa gctctccaag tgttcgcgca
     4141 ctcgtttgct ctcggacaaa tccggcaacg cgctcaaggc ggcgcgccga ccgcgcgtgg
     4201 ggtggccgta gcgcgtgtgt tcgatcacca aatcgagcgc ggcttcgtcc cgcagcgcgc
     4261 ccatcccatc gagcgcgccc atgcgaacga tgtcggccca agaatcacgg ctcaagagcg
     4321 aggtaagcag cttcagtgcg cccttctggc gcgtgcgccc gagcgagcga gccgcctccg
     4381 attctacgag gtaactcgga tctttggctg cgagcggacg caacagatcg aaggcccgct
     4441 cgttgcggaa ctgtcccagt gcgccggcga ctgcgcgtcg caccttgggg tgcttggttt
     4501 tgacatgctt ggctagctct tcgagcgcca gcgggctccg ggtcgagccc aatgcgcgcg
     4561 cggcttcgac gcgaaccatc cagggctgac tctccgtcga caggcactgc cccagcgcgc
     4621 cgagcgccgt ctgatccggt ttgcgcgcga gcgcctccgc cgccacccat ttcaagagcg
     4681 ccgagctgcc gtgcgcgagc tggttacgca gcatgtccga aggagcctcg aaaccaatac
     4741 tcccgatcac ccgatattcg gggtcgaacg ccacgtactg gggccggcgc ggcagcggca
     4801 cggtcaagga atcctccgct cccgagcagc gcttcttgtg gcgggtgcgc tctcctgcgg
     4861 agtccaggat ctcgatctcg aaatcgaact cgaagatcgc gttctcctga ggcttttggc
     4921 tttgcttcaa ggccacgctg accaggccgt cttcgtagct gaccttgacc ttcagatcgg
     4981 ggtgacccgc gcgatagacc caatggtcga aaaactgttc caacgagcgc cccgaaactc
     5041 gctcgaacgc gcgcatcaga tcgttggtct ccacgatgcc attggcgtgc tctcgcaggt
     5101 agctcgtcac gcccgcgaag aacagctggt ccccgagctc gcgccgcagc atgtgcagca
     5161 cgagcccgcc cttctcgtac agatgacgat cgaacaaatc gatcggctgt gaatagtctc
     5221 tacaaacgat gggtcggcgg tatcgccccc ccgcctccga gaggtacgag ttcacgtcgc
     5281 cgcgaacccc gtagtcgtat tcatcccgcc ccaggcggtg ctctcgttcc acgtgttcga
     5341 agtaggtcgc gaagccctcg ttcaaccaag catgggacca gtcgcggcag gtcacgaagt
     5401 ccccgaacca ctggtgcgcg agctcgtgcg cgaccaagtc gttggaagtg acatccaggg
     5461 ttgcccgcgc gtccagcagc acgtgctcgt agagcgtggt cgcggtcgtg ttctccatgc
     5521 ccccgaacac gaaatcgtgc accaccacct gcgaatagcg gctgaacggg aagggcacct
     5581 gcgtgagccg cgagaatagc tcgatcatgc gcggcgtctc ccccaggctg cgtttggcgt
     5641 ccttggcgcg cgagaccggc acatagtaac gcaccggaat tcggcggccg tcctcgagct
     5701 ccgcgtcacg gtctttctcg atcgcgaagc ggcccaccac cagcgtcacg aggtagctcg
     5761 ggtgtgggct atccagcttg aagtgaaatg tgccgacccc attcttcgct ctcttctcgg
     5821 acaacaagtc gccgttcgaa agcgccacga actctgccgg taccttgacc ctgagttcgc
     5881 tcgtcatttt gacgtgcggt ttgtcgtgac atgggaacca gtgccgcgcg tcttcgtcct
     5941 gacactgagt ccaaacctgc accggtcggt ccttgacttg cgcgtccggc cctagaaagt
     6001 acagcccgcg ttgcggcacg gcacggtaaa ctatctcgat gcgggctcga tctcgcccct
     6061 cgaaccccga gacgcgcagc cgctcaccgt cgtattcgaa gggcagcccg cgctgatcgg
     6121 cagcttcggc cagggccacg ctcgcgattt cgaacccgat cgcgtccaag tccagattga
     6181 ccgcgtcagg cgccatgcgc tcgatctcca agaacgcccg cccggatacc gagcgtctcg
     6241 cgaaatcgag ctcgatatcc aagaaagtgt gccgcactgt gaacgggcgt gagcgttcgt
     6301 attttcgttc ggtaccggcg agcgagaatg ggctaggacc cagagcggag caggcgcagc
     6361 gctcatggag gcgttcgaag cggctcatgg aagcctattc gcataccggc tattcggctg
     6421 agtcgcaagg tcacgaggac ggcgcccggg cccgccgcgg aagaaaggcc gaacgagtga
     6481 ttccgaagga gcggcaagcc agcaacgaga ggctatagtc ccgccgtgcc cgtcacgctc
     6541 agcggagaag tcgagcgcgt caccttcgag aatgagacca cgagcttccg agtgctccgg
     6601 gtgggctcgc tggaagggga cgcggggcgc cgcggcagcg tcgtcgtggt cgggacattc
     6661 caagccgttg ggcccggcac gcgggttcgt atcacgggtg agttcgttca agacgcgagg
     6721 cacggtgaac agctgcgggc ggacgtgctc gtgccgctcg aaccgaacac actggtgggg
     6781 ctcgagcgct acctgggctc tggtctgatt ccgggtattg gtccttcgct cgccaagcgc
     6841 atcgtcgacc agttcggcct ggacactttg aaggtcttgg acgaggcccc cgagcgtctg
     6901 gcgggcgtgc ccggactcgg taagcgacgg gtcaacgaca tcaaggagaa ctgggcgttg
     6961 cagcgcgcgc tgagcagcgt gctcgtgctg ctcagaaccc acggtgcgtc acacaacttg
     7021 gcgttgcgga tttggaagca ctacggcgat cgcgccgcca gcatcgtgca gcgttcgcct
     7081 tatcgtttgg cgctggacgt gcgcggcgtg ggcttcaaaa ccgcggatcg cttggcgcaa
     7141 tcgatcggca tcgtctccga tcacccggag cgctgtcagg cgggcgtgct ccatgtgatg
     7201 gaggaaattt cggaagcggg ccacatccac acgccacgtg cggacgtggt ggcccacacg
     7261 gcggagcttc taagagtcga tgccgctcac atcgaagctg ccgtcgacgt gttgtacgca
     7321 gccggccgcc ttcgggtgga acaaggcagt ttattcctcg agcagtttta cagcgcggag
     7381 gtggagactg cgcagcacat cttgagtcgc accggtcacc ggcaggctac gcaaatgccg
     7441 gcgggcaacg tcgagcgagc catcgccgct ttcgaggcac acagcaagat cagcttggcc
     7501 tccacccagc gtcaggccgt cgaactcgcg gcccgcgaac gcatcgcgat cgtgaccgga
     7561 ggcccgggcg tgggcaagac gaccatcgtg cgcgccatgc tgcgtgtcat cgagagccaa
     7621 cgcctgaaag tggccctagc cgcgcccacg ggtcgtgccg ccaagcgcct gtccgaagcc
     7681 acgggtcacc cggcgagcac gctgcatcgt ctcctggagt tcgatccacg cagcggttgc
     7741 tttcagcgca acctcgcgtc cccgctcgag gcgcacgcct tgatcgttga cgaggcttcg
     7801 atgatcgacc tagaaatgat gcaggcgctg ctgggagcgc tctcgcccag cgccaagctc
     7861 gtgattgtcg gggacgccga ccaattgcct tcggttggtc ccggcgccgt gctgcgcgat
     7921 ttgatcgaaa gccgcgtcgt cggcaccgtg cgcctcaatg aaatcttccg gcaggcggac
     7981 acgagtggca tcgtcgaaaa cgcgcaccgc atcctcgctg gcgaagaacc caagaccgcc
     8041 gcgcccgaag atccacgcgg cgactttttc gtcatcaagc gtcggacgcc cgaggacgcc
     8101 gctcaggtca tccgcgaact cgtcaccgag cgcatcccca agcgttttgg gctcgacgcg
     8161 cgtcgtgaca tccaaatctt gagcccaatg caccgggggc ccgccggcac cttggccctc
     8221 aacgagatgt tgcagtctgc tctgaacccc gacggatccg cgatcgaggt gagggggcag
     8281 aagcttcgcc agggtgacaa ggtcatccag acccgcaacg actacgaccg cgacgtcttc
     8341 aacggtgacg tcggcagcgt gctccaggtc gatacgaaag aacgtaaact cgtggtgcgc
     8401 ttcgatcagg gagacatcga atacgacgac tcgaacctag cgtccctgaa cttggcgtac
     8461 gcgcttagcg tgcacaagag ccagggcagc gagtaccccg ccgtcgtgat ccccttactc
     8521 acgacgcact tcatgatgct caaccgaagc ctgctctata cggcggtgac tcgtgcacgg
     8581 aagctatgcg tactcgtggc ggatccgcga gcgctcagct tggcacttgc cgacacacgg
     8641 cgcgaggagc gacgaacccg tctggcggca cgactacaag gcgatgcagg ggccggcaag
     8701 ctgtagaggg ctcggtgtgc gcggtgatcg cacgagcacg gacacacaaa acgaacgaag
     8761 ccacccgagg gtggcttcgt tgaacagcga acgctgtatt atgtacggat tttcagctca
     8821 gtaacggttg cggccgccac gcgatccgcc gccgccgcca ccgcctcgga agccgccgcc
     8881 gccaccgccg ccaccagtac gctcctgcgc ctcatcgacg cggatttggc ggccgtcaag
     8941 aatggcgccg ttgagctgct cgatggcagc ggccgcggcg tgcgagttcg ccatcgtgac
     9001 gaacgcgaag ccacgcggct gaccggtgga acgatcgagc ggcagcgcca cttcacggac
     9061 ctcgcccgcg gcctggaagg cgctctcgat agaagcttgg ctggtggaga aagacagatt
     9121 gccgacgaaa agacgattgc ccatgtgatt gatgtcccga ctggcttcag gcgctccgcg
     9181 ccttcgatca atcgcgaggt gaagccgacc tctgcgatcc gctgcgctga tggcccaaac
     9241 tcgggatatc aaccacagct acttgtgcgt catctagtct tccaggcgag ccgaacgcgc
     9301 tgacagagtg cgattacact catgggaaga gcgaggcata ccaagcagcc gcagacgtat
     9361 cagtggcctg gaacataggc tcgaattggc cgatgcacaa gggctaactg tgaaaaacac
     9421 ggatcgcggg cgcagacagc tagaaaaatc tacgaccatg catggcggct tacccgcggc
     9481 gaattggccg gacgccggaa ctggtgccga agaagtgagg taaagcctca cctctttcac
     9541 caacttgcct ttcaccaacc tgcctttcac caaccgggcg atcttacctt tcggcagcgt
     9601 gctatgcccg ggcacgggac gcttcggcgg gcttcgcgcc ttgagtgacg tcccgctcga
     9661 ggtccggaac cggctaggtg agtgccggac tagatcgccg gcaggcgatc gaggcgattc
     9721 tgattgtcct cggcagtgct ggtctgcaaa cagccattta cgacacgcag ataagcgcgt
     9781 cggccaattt cctcgacata atagctgctg cgattgcggt cgatgtcgcg aatcacgtct
     9841 ttcttacgac gcggggacca gttctcgccc gcgttgcaca aagctacgat atttcccgtt
     9901 cgatccttct taaccgcttt gactcgacga tccacttgcg attcgctttc cgactggagg
     9961 actcgcggtc gaccagttca aactgactag ggcccaagtg tcggctttga actcgtgctc
    10021 agctccgtag cggcgattcc gcaggtggca gtgcgtcgcg gagctgtaaa cgatgcatgt
    10081 ctgtaggcga cccgcatgaa tcgatgtcag cgcacgggcg ctgccagacc ggaaggtctt
    10141 actgagcaat tcgttctcga atgcagccga ttctcgaatg catggcctcc gctgctccgg
    10201 aagggagcgt caaacgccgc cgccagccca gcaggggaat cagaatttcc cgagtctgca
    10261 ctgatcggca agctcggctt tcgtgagcaa gctcgggctt cgagcgtgag tcgcgagtat
    10321 ggcacgttct atcgtttcac gcccctgcca aatagctttt cgcgactctc cggtgaatcc
    10381 cgaagcctat tctctctaac ccgaattctt cgcgcgcatt cccccgagtg ggccaagcag
    10441 gggcttggtg tgctgaaggc tcaggcttga ttccgattcg gcgtgccttt cgacgctacg
    10501 ctaaggtctt cgacgctacg cgaaggtttt ggacgctacg ctaaggtctt cgacgctagc
    10561 caaggtcttc gacgctacgc gaaggttttc gacactacgc aaggtgccca gctcgccggc
    10621 atggtagata atgccggtcc aatcagtgct cttggcggtt tcggctttgg gtctgctgcc
    10681 caggttgctg ttgggcggtg tgtcacgcgc gcaggccttg ccgttgagca ggcgtacaaa
    10741 gcgtgccgcg ttgcgatgca accgtgcgga atgcaaccgt gcggaatgca accgtgcgga
    10801 cttcgcgcgt gtttggctgg atatgcgaaa caatcttcga ggtgaccgta catgaacagt
    10861 tcgaaatcgc cagcacgtgg aaaagagtcc aaaccggagt gcctcgccat tgcggtgcag
    10921 tatcgaaata aacgagggat ggtgtacgag ctgaattcgg tggatggttc cgtggatatt
    10981 cacgttctcg aaaagtccca ttcggaaccg caagttcaca tcgaagccca tcatgggcgt
    11041 ctcgccgacg cggtagttat ttccaagaac ggcaataact gcagggatgt gctgcgcgaa
    11101 atcggcgccg aatgggaaac gcggcgatct ttgccgcacg tcaattggga cacggtcgca
    11161 gcgttgctgt cgagcgtacg agcaatttga gccgcccgct cgttcgcgac tcggcgttac
    11221 atgcccccta ggtggcccgt ttttggggtt gaggcgcgcg cgtggaaaag ggcgcgccat
    11281 gtcgaccgtc acggcactgg ctcagccctt tcccgatgaa gaactcacgt caccgcgcaa
    11341 gcggccccga ataacgcctc cggagccgca gaatcctcac tgtgcaacga ccgtccacgc
    11401 tggctcgacg ctcgtcaccg tcgtaggtgc tccgactagg tcgaacaacc cttggctggg
    11461 tacctgatat tgaatcccat tcagcttgaa caggaactgc tgcttggccc agagcttcct
    11521 cacggctgcg tagtagatcg gtcgcccacc atccttgccg ttcgacacgt tgctgatcgc
    11581 gtactgagtc tcgggaccgt cttcggcatc gcttcccgga tccgcgagct cagtctgggg
    11641 cgcgggcggc gcagcttgcc cgggtgccgg atcgagcgaa acggcctcgg gaaactgatc
    11701 ccgaaccgga acgctcgaag cctctacgac cagcgcgtac gaggaggtac tggtgcaact
    11761 cgcaagtgca ggctccagct ggcgagcgtt gacgacctcc gggcgcgagt ctggaggact
    11821 cgatcttcgc cagacgtact cacagaagtc caacaccgga taggtcttcc atcctggtgc
    11881 tctcgggcat ttcccatgga cttggggtcc aaaccagtcc gtgcagatgg agtgagcgtg
    11941 aaccgcgagt gacggcgatg agtcgaggtc aaggctcgaa gtgacgagcc cctgagagat
    12001 accggactgc gcctcgatat ccccgtccgg ggcggcactg gcgcaagcgt tgagtaacat
    12061 cacaccgagg atcgagaaca gcatacgttg catgtcctcg tgtattgaac gcgtggcgcc
    12121 gcgttctctc gcccctcgtc gcagtcattt gtttgcggca gtcctgccta gtcagtctgc
    12181 ctagtcagtc ctgcctcgca ctcgagcatc ggctgagtcg attgaactta cgctcatgct
    12241 caccggcaag agaccacgat acgctgctct tcgtgatggt cgatgaggac gttgcgctcg
    12301 tgcgcagagt gctgggcgga gaagccgagg cgcacgccga gttcgtgcga cgccttagcc
    12361 atgtcgtgcg tcaccgcgtc gcgcgggtgc tgatgggagc cggcgcgaag cctggctcga
    12421 cacgctcgcg acaggcgctg ctcgatttga tgcaggaagt ctacgtggtg ctgctggatc
    12481 gaaagggtcg cgtgctctcg acctgggatc ccgcgcgtgg gctctcgctc gagaacttcg
    12541 ttgggctagt ggcggagcgt gaggcgattg ccttccaacg tagcgggcga cgtagcgcgt
    12601 gggcagagca accgactgac gatctcgtcg atcacacgtt gctacgcacc tcgcccgagg
    12661 acgaggccgc cgcgaaagaa caactcgagc tgttgctcga gcacctctcc gagcgcctga
    12721 gcccacaggg cttgctcctg ttcgaggcgc tgttcgtttc ggagacgccg gtgcccgaga
    12781 tatgcacgct cttcgccact acaccgacgg ccgtttacaa cttcagagct cgtttgaagc
    12841 aagtggtttc agattggaag cggagatcgg agcgaactca tggtgcgagc actcggaatg
    12901 tggtcgatgg ggggcgcgtg ttgaggagtg agccatgagc aagaagtctg gtgccgacgc
    12961 cgagggcgtg ttgcgtcagc tcgctgagct tcataccgta cgcactgctc ggcttcgcga
    13021 ttgcgacccc gacgcagacg gcgcggacgc ggcattcgct cggctgacag acgaggaaga
    13081 gtcgcgactc gtggaagccg tcgtgcgacg cacgcgggaa acgcccgcgc gacgacctct
    13141 cgcaggtgcg gtggcgaacc cggaccaagg gcttcggcgc ttcgggttcg agcgagggcg
    13201 gcggtatgcg ggggcatggg cggccgccgc aactctgatc ttggctggcg gtgccgtgta
    13261 cggtacgcga gcggccccgt catggcccga gtatgtgctc gcgagccccc catcggacgc
    13321 gcagttcagg gccgccgtcg acgcaagcgt gacggcggag tcggacgccg tcgatcacgt
    13381 atacgggatt ggtcgcgagc tgcgtttcgt ggtccgccca cgacatcctt ttgcggaggc
    13441 gccgtccgtt cgggtttatg caggctcgaa tggcaccgaa gcactcaggg cggtgcggct
    13501 cgagccactc gagggcggct cgattcgcgt cagcttgaag acgggcccgg gcggctatgt
    13561 gccgcgcccg gggcccgacc agttgttgat cgtcttgggt gaaaggtccg cttggagaag
    13621 tttggtgcga agactctccc atcctgccgg agacgtcgta cagagctttc gggtgccggt
    13681 gacgtggcac cgggtcaacg agtaagatgc gcctgcgaac gcctcggctt cgcggcgtat
    13741 ccagggcaat ggcgacggct ttcttggccc tgttctcggc gcaagcttcg actggccaga
    13801 gcacgagcgc agcgacggtc caggacgact cggtcgccgc ggtctcaggg tgcgcggccg
    13861 tggttggtac tcggatttgt gagctgagcg cgggctcgga gcgtgagctg cgtgtttggt
    13921 tgccgcagct cagcgacgaa tacgcacggt tgctcgtgca gctagatgga cgaaaggtct
    13981 cggcctttcg ggtgacaccc tccgagcacg gatatcgcct gacgattccg atcgagctga
    14041 cgcgtgatcg ggcccagctc tcggttttca tcgaccaccg ctcgattctg cagctagacc
    14101 tgcggcgcgc gggcgagtgg ccttggcaac gcgagcccgc gggcgcactg cggcccgcaa
    14161 ccgcagcctt acgagaccga ctccccttgg cccgtggcgc ggagcgctcc aagttgttgc
    14221 gcgcgctggc tcaaacagaa gtcgcggcgg gtcgactcga gcctgccctt cgattgttcg
    14281 aagagggcgc acgcggggct gaagaacagc aacggcactc ggacgcgcgc ggccactggc
    14341 ttcagcgcgc gtaccaccaa gcgcacgccc acgacttcgg cgccgcgcgg gcttcggtcg
    14401 cccacgcgga ccaagtgtcg tccgcagcgt tcgtggacgc cgacggcagg gcggcagcta
    14461 gctacgtgcg cggcgtgttg gccgcacaaa tgcgcgatct gcgcggagct caagccgcct
    14521 acgaagaggc gcaacgctgg gtgaatcgtg gagcgacctt gatgcggcct cagcttgcgg
    14581 tggcgcaggc cttcgttgcc gaacaacaag gacgtctggg cgatgcagtg gcaatactcg
    14641 aacgcgaact ggaggctgac ctcgccaaga gttcgccttg cttccacgcc atcgtgctca
    14701 cgaatctagg gtggtacgag ctcctgcgag ggctcaccga aggtcgttcc tcggtctccg
    14761 caccacgcgc gcagcaacgg c
//
LOCUS       DASTKU010000105         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1804577, whole genome shotgun sequence.
ACCESSION   DASTKU010000105 DASTKU010000000
VERSION     DASTKU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804577"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFQ61_03755"
     CDS             complement(<1..464)
                     /locus_tag="VFQ61_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953590.1"
                     /translation="MRFSVWSKQSQLALGVSLLASVGAARADVVVSPSASLHSAPLGP
                     APPQPPAQAGAPPSDSPQKPVSPAVSPPGLPTAPGVSATPGLPAGPGFPAGPVAEPTL
                     PGDTVLDRPVLVPVPGDKPVLVLHAPATNTQAIVYLHGLCGNVRAVESWKEAA"
     gene            759..1595
                     /locus_tag="VFQ61_03760"
     CDS             759..1595
                     /locus_tag="VFQ61_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953591.1"
                     /translation="MSSWRAMFPVAAGLVGVMLSAGCAAGQHECQANTWSGSCELMQA
                     TLVRHLEVPVASVVYQATYRTLPDPQNPNAGAPGLNVQKEFIARDRLETALKTHIETH
                     HTVPCYVTPPPAHACTPGTLNVEVPEFDALHAQVDEEEGPKGCAQIEARSSQDRLTKT
                     SSGATSSINEFFEFPVGSADLTPEFGAVLDGVAQRLKGNSAIQCVGVVGAWTRGENMG
                     LAFSRARTIREELLKRGVPPERMIALTVDPRPPSATAGAEPPSSDERRVNLKVLLEVP
                     AP"
     gene            complement(1633..>2827)
                     /locus_tag="VFQ61_03765"
     CDS             complement(1633..>2827)
                     /locus_tag="VFQ61_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953592.1"
                     /translation="VSVEELRAKGRFRRLAMSRRPTPGEEHAPKVDRNSLREAVLGAG
                     EYLARNVRLDGSYAYEIDGQSGEESQDYNLPRHSGATWFLAEAAGYSRDPKLERSVRR
                     AARYLARTALVDCGERRCIAQREQADLGSSALALLAFAELVQNGVAPELLEDAVSLAE
                     FLVSQQRPDGEFQHLYDRVERHPIDVQLLYYTGEASYALSKIYRITHDPRYLAAARGA
                     LGHLVKQPIWAPALRYYWGAEHWTCHALEDLWDAAPNHEALEFCLRWLEFVGRTAVSG
                     REVAPEYDGASATGPFLPPQLVATATRLEAAVATLAVARKAKLEPARIDRLESEIRAA
                     FRFLMRYQFLPGPMHLMRDPERMYGGVPASETDFRVRIDYPQHAGTGLLAYLKQLEQG
                     RRATQ"
BASE COUNT          507 a          896 c          920 g          504 t
ORIGIN      
        1 gctgcctctt tccacgactc gacggcgcgc acgttcccac acaagccgtg caaatagacg
       61 atggcctgcg tattggtcgc gggcgcatgc aggacgagca ccggcttgtc tccaggaacg
      121 ggcactagca ccggcctatc cagcaccgtg tcccccggca gcgtgggctc tgcaacggga
      181 ccagcgggaa atccgggccc agcgggcaac ccgggtgttg cggatacacc gggtgcagtc
      241 ggcaatcccg gaggggagac cgcgggactc actggtttct ggggcgaatc ggacggcgga
      301 gcgccggctt gcgctggcgg ctgcggcggt gcaggaccga gcggtgcact atgaagtgag
      361 gcgctgggtg aaaccacaac gtccgcgcgc gccgccccga cgctcgcgag caacgagacg
      421 cccaacgcta gctggctttg cttgctccac acggaaaacc gcatttcgac cgctaggttc
      481 ttatgatgga aatgtcgaaa ccaggcaagt attgataggc tcgagttgcc atgtttcccg
      541 aactgcttcg acgtgcctcg ctttgtagtt cctcgctatg ccgtgctttg agctgccgcg
      601 atgttttcct ccgtcgcgct ctcgctcctg agctgtgggc gcctagagtc tcggccccgg
      661 ctgtccgcag ccctgctctc cattctgtcg ttggcgcgtg tcctttaccc aaacttctgg
      721 gtgtctggga attgcgaagg cgcacggagt tcgagagtgt gtcctcatgg cgcgcgatgt
      781 tcccagtcgc ggcagggctg gtcggcgtga tgctgagcgc gggttgtgcg gcagggcaac
      841 acgagtgcca ggccaatact tggtcgggaa gttgtgagtt gatgcaagcc acgttggtcc
      901 gtcatctaga ggtgcccgta gcgagcgtcg tctaccaagc cacgtatcgg accctacccg
      961 atccgcagaa cccaaacgcc ggcgcgccgg gattgaacgt tcagaaggag ttcattgcgc
     1021 gtgatcgcct ggaaaccgcg ctgaagaccc acatcgagac gcatcacacg gttccttgtt
     1081 atgtgacccc accccccgcg catgcctgca cgccgggcac gctgaacgtg gaagtgcccg
     1141 agttcgacgc gctgcacgcg caagtggacg aagaggaagg ccccaagggc tgcgcgcaaa
     1201 tcgaggcgag aagtagccaa gatcggttga ccaagaccag cagcggggcc acttccagca
     1261 tcaacgagtt tttcgagttt ccggtcgggt ctgccgatct gacccccgag ttcggcgcgg
     1321 tgttggatgg cgtggcacaa cgtttgaagg gcaactccgc tattcaatgc gttggagtgg
     1381 tgggcgcttg gactcgcggc gagaacatgg gactcgcgtt ctcgcgagct cgcacgatcc
     1441 gggaggaact attgaaacgc ggagtacccc ccgagcgaat gatcgcgctc acggtggacc
     1501 cccgcccacc gtctgcaact gcaggagccg aacccccttc gagtgatgaa cggcgcgtga
     1561 acttgaaggt gctgctcgaa gtgcccgctc cctgaggctg gggctcaagc ctgaagccgg
     1621 gcagattgag ttctactgtg tcgctcgtct gccctgctct agttgcttca gataggccaa
     1681 caagcccgta cccgcgtgtt gcgggtagtc gatgcgcacg cggaaatccg tttcgctcgc
     1741 gggcacaccg ccatacatgc gctcgggatc gcgcatcaag tgcatggggc caggcaggaa
     1801 ttggtaccgc atcagaaagc ggaacgcggc ccgaatctcg gactccaggc gatcgatacg
     1861 agccggctcc agcttggcct tacgcgccac ggccagggtc gccaccgcgg cttccaagcg
     1921 tgtcgccgtc gcgaccagtt gggggggtag gaaggggccc gttgcgctcg caccgtcgta
     1981 ctcgggcgcc acttcacgac cggaaacagc ggtccgcccc acgaactcga gccaacgcag
     2041 gcaaaactcg agcgcctcgt ggttcggggc tgcgtcccag agatcctcga gtgcgtggca
     2101 cgtccaatgc tccgcgcccc aatagtagcg gagcgccggt gcccagatcg gctgcttgac
     2161 gagatggccc agcgcaccgc gtgccgcggc gagataacgc gggtcgtgcg tgatgcggta
     2221 gatcttcgag agcgcatagg acgcctcacc cgtgtaatag agcagctgca cgtcgatcgg
     2281 gtggcgctcg acgcggtcat acagatgctg gaactcaccg tccggccgct gctgactgac
     2341 gaggaactcc gcgaggctca cggcgtcttc gagtagctcg ggggcgacgc cattttgaac
     2401 cagctcggcg aacgcgagca gcgcgagcgc ggacgagccg agatcggctt gctcacgttg
     2461 cgcgatgcag cggcgttcac cacagtcgac cagcgcggtc cgggcgaggt agcgcgccgc
     2521 gcgcctcacg gaacgctcga gcttcgggtc tcggctgtag cccgccgcct ccgccaaaaa
     2581 ccaggtcgcg ccggagtgcc tcggtaggtt gtagtcctgg gattcctccc cgctctgacc
     2641 atcgatctcg tacgcgtacg agccgtcgag acgcacgttg cgagccagat actcgcccgc
     2701 accgagcacg gcttcgcgca agctattgcg atcgaccttg ggggcgtgct cttccccagg
     2761 cgtcggacgt cggctcatcg cgagccgacg gaagcggcct ttggcgcgca attcttcgac
     2821 gctcacg
//
LOCUS       DASTKU010000106         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_689470, whole genome shotgun sequence.
ACCESSION   DASTKU010000106 DASTKU010000000
VERSION     DASTKU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689470"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..>2811
                     /locus_tag="VFQ61_03770"
     CDS             232..>2811
                     /locus_tag="VFQ61_03770"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET9953593.1"
                     /translation="MSELPKAYEPKEVEPRWYQFWVDHGVFRANASPEDTRPVYTIAM
                     PPPNVTGSLHMGHALFGTIQDVLSRHKRMQGFNVLWQPGIDHAGIATQIVVERKLQRE
                     GKSRHDIGREAFVSEVWRWKEQSGGRIAEQMRVLGCSADWGRTKFTMDPDMSRAVREA
                     FVRLYEEGLIYRATRLINWCSSCRTALSDLEVENEEAQGELYEFAYPVADVDPGHGAT
                     ELVVATTRPETMLGDTAVAVHPDDPRYTHLHGRKLRHPFVDREIPIILDPVLVDMSFG
                     TGAVKVTPGHDFNDFATGKRHGLQEINILELDGKLNAEGGQFAGMTVKEARHAVKREL
                     EQRGLARGGKPHALSLPRCGRCATVVEPMISTQWFVKTTPLAEPALEAVRSGRTQILP
                     EEWVKTYEHWLVNIQDWCISRQLWWGHQIPAFFCGACNHVTVSREDPTSCPKCGSQQL
                     TRDSDVLDTWFSSALWPFSTLGWPEQTDFLKRFYPSSDMETGYDILFFWVTRMMMMGL
                     HFMGDVPFRRILLHGMVVDETGEKMGKLRGNTIDPLDLIHGAEFAHVVQKLMPDAPLE
                     DALAKFRRAYPSTAQMGQGFAAQGADALRFTLCSYSPQAKRIALSPARIDGYRRFCNK
                     IYNATRFALPYLAEVKLGDGEPPKPQLLINAWIRSRLGVAVTESTRGVEEFRLDDGSS
                     ALYHFFWDEFCDWYIEMAKSIFQNGSAAEQEETRVTLASVLETALRGLHPYMPFITEE
                     LWQRVPRPPASPISIALAPYPTLQPGARDQAAEARMNVLMAAISAARTARSEHEVHPA
                     AKVPLELRTGNPELRELLTSEARFIEHLVGTQGKVVIGAAHGERPRGTVVSVTGDVEV
                     LVNL"
BASE COUNT          543 a          893 c          887 g          488 t
ORIGIN      
        1 gaaggtcgtt tgggaggtcg ttccgccctg ggccgagacc atgatccggg aagaacttca
       61 gcgtctgaca cgcgactagc caaggctgaa cgcggaacgg agcgaatcac tcgggcgact
      121 cgggcgcccg atcctcgctc ctctcgcggc ggaccgagct gctcgatgct cgtgacgccg
      181 caaacctgcc tttgaaaaag accgggttcg ggtctaggtt tgcgggcccc catgagtgag
      241 ctgcccaaag cctacgaacc caaagaagtc gagccccgct ggtaccagtt ttgggtcgat
      301 cacggcgttt tccgcgccaa cgcatcaccc gaagacacgc ggcccgttta cacgatcgcg
      361 atgccgcccc cgaacgtcac gggctccttg cacatggggc acgccctgtt cggcacgatc
      421 caagacgttc tctcgcggca caagcgcatg cagggcttca atgtgctctg gcagcccggc
      481 atcgatcacg ccggcatcgc gacgcaaatc gtggtcgagc gcaagctgca acgagaaggc
      541 aagtcccggc acgacattgg tcgcgaagcc ttcgtttccg aggtttggcg ctggaaagag
      601 caaagcggcg ggcgcattgc cgaacagatg cgtgtgctgg gctgttccgc ggactggggc
      661 cgcaccaagt tcacgatgga tccggacatg tcccgcgcgg tgcgcgaggc gttcgttcgt
      721 ctgtacgagg aagggctcat ctatcgagcc acccgcttaa tcaattggtg ctcgagctgc
      781 cgaaccgcgc tcagtgattt ggaggtcgaa aacgaggaag cacagggcga actttacgag
      841 tttgcgtatc ccgtggcgga cgtcgatccg gggcacggag cgaccgagct cgtcgtagcc
      901 acgacgcggc ccgaaaccat gctgggagac accgcggtgg cggtgcaccc ggatgatccc
      961 cgctacacgc acctgcatgg ccggaagctg cggcacccct tcgtcgaccg agaaatcccg
     1021 attattctcg atccggtgtt ggtcgacatg agctttggaa ccggcgcggt gaaggtgacg
     1081 cctggtcacg acttcaacga cttcgcgacc ggcaagcggc acggcctgca agagatcaac
     1141 atcctggagc tcgacggcaa actgaacgcc gagggcggtc agttcgcggg catgacggtc
     1201 aaagaggcgc gccacgccgt gaaacgcgag ctggagcagc ggggcctggc tcgcggtggc
     1261 aaaccgcacg cgctcagcct gccccgctgc ggacgttgcg ccaccgtggt ggagcccatg
     1321 atttcgacgc agtggttcgt gaagacgaca ccgctcgccg aacccgcgct cgaagccgtg
     1381 cgcagcggac gcactcagat tttgcccgag gagtgggtca agacttacga acactggttg
     1441 gtcaacatcc aagattggtg catctcgcgc caactctggt ggggccacca gatcccggct
     1501 ttcttctgcg gcgcctgcaa ccacgtcacc gtgagccgcg aagacccgac gagctgcccg
     1561 aagtgcggca gccagcaact gacgcgcgac tccgacgtgc tcgacacctg gttttcgagc
     1621 gcactctggc ccttctcgac cttggggtgg cccgaacaga ccgacttctt gaagcggttc
     1681 tatccgtcga gcgacatgga gacgggttac gacatcctct tcttctgggt gacccgcatg
     1741 atgatgatgg gtctccattt catgggggac gtgccgttcc gacgcattct gctgcacggg
     1801 atggtggtcg atgaaaccgg cgaaaaaatg ggcaagttgc gaggcaacac catcgatccg
     1861 ctggatttga tccacggcgc cgagttcgcc cacgtcgtgc aaaagctgat gccggatgcg
     1921 ccgctcgagg acgcgctcgc caagttccgt cgcgcttacc cgtcgacggc tcaaatgggg
     1981 caaggcttcg cagcccaggg cgcggatgcc ctgcgtttca ccctgtgcag ttattcgccg
     2041 caggccaagc gcatcgcgct ctcgcccgcg cgaatcgacg ggtatcgcag gttctgcaac
     2101 aagatctaca acgcgacgcg cttcgccctc ccctacttgg ccgaggtgaa gctgggggac
     2161 ggtgagccac cgaaaccaca acttctgatc aacgcctgga ttcgatcgcg gctcggggtt
     2221 gcggtgacgg agagcacgcg cggcgtggag gagttccgct tggatgatgg ctcctccgcc
     2281 ctgtatcact tcttctggga cgagttctgc gattggtaca tcgagatggc caaatccatc
     2341 ttccagaatg gcagcgccgc ggaacaggaa gagacccgcg taactctcgc gagcgtgctc
     2401 gaaaccgcgc tgcgaggact gcatccatac atgccgttca tcacggaaga gctttggcag
     2461 cgggtcccac gtccccccgc gagcccgatt tcgatcgcgc tcgcgccata ccccaccctg
     2521 caacccggcg cgcgagatca ggcggccgag gcgcgcatga acgtactgat ggccgccatc
     2581 tccgccgcgc gtaccgcgcg cagcgagcac gaggtacatc ccgccgcgaa ggtaccactc
     2641 gagctgcgca ccgggaaccc agagctccgt gaactcctga ccagtgaagc tcgcttcatc
     2701 gaacacttgg tggggaccca aggtaaggtc gtgatcggcg cggcccatgg tgagcgcccg
     2761 cgcggcacgg tcgtgagcgt gaccggagac gtggaggtgc tcgtcaatct g
//
LOCUS       DASTKU010000107         8256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1359073, whole genome shotgun sequence.
ACCESSION   DASTKU010000107 DASTKU010000000
VERSION     DASTKU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8256
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359073"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..980
                     /locus_tag="VFQ61_03775"
     CDS             138..980
                     /locus_tag="VFQ61_03775"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET9953594.1"
                     /translation="MPDPDWTSCLDNLGVPAHALRVLSNGALPLYAQIARRLRAQIQA
                     GQHARGARIPSEHDLAAQFAVGRPTVRQATELLVQEGVLERRRGSGTFVSELPKEVDV
                     FSAFGTLASLAQSELPVSVRLLGKVRKIAPPEAARARLKEGRAFFVQRLCSLAGKPLL
                     LEEMYFEPEVFPGLDVMPLKGRSLSELSREHFQLRPVSAEQRFSLFALDEKRAQWLGL
                     PVGREVLKVERCLDFAGAPRAHYAELFCRTDQVCFTQHLSFGATSKASTLPDRGRTQA
                     GASR"
     gene            994..2226
                     /gene="trpB"
                     /locus_tag="VFQ61_03780"
     CDS             994..2226
                     /gene="trpB"
                     /locus_tag="VFQ61_03780"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET9953595.1"
                     /translation="MTLDPVYFSEFGGTFMPEILVSTFEDLTRIYQEARRDPKFFAEY
                     EQLMRSYSCRPTPITFAENLTRHFGGARIYVKREDLNHTGAHKLNNVMGQGLLVRRMG
                     KKRVIAETGAGQHGVATATMAARLGLECTIYMGEDDVRRQRPNVFWMERLGATVVSVK
                     DGSRTLKDAINEAFRDWVANMDNTHYVLGTACGPHPFPEMVSWFQSIIGKEAREQMLA
                     IEGRLPHHVYACVGGGSNAMGLFSGFMEDKDVELVGVEAGGHGLETGKHAARLSGTDA
                     SIGVAQGYRSYFLQDEDGQMLDTHSVAAGLDYVGVSPILAHLKHIGRARFEVATDQQA
                     IDALALAARKEGLIPALESSHAFACAFREAPLHKDQIILINQSGRGDKDIFTVADAFG
                     DPAWKEFIRRKASEYAAE"
     gene            2213..2977
                     /gene="trpA"
                     /locus_tag="VFQ61_03785"
     CDS             2213..2977
                     /gene="trpA"
                     /locus_tag="VFQ61_03785"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET9953596.1"
                     /translation="MQLSEYIRAQREKQRILLMTHIVVGYPNLERSRELVDAMVAAGV
                     DLIELQIPFSEPVADGPVILRANQEALKAGTRVADCLEFARDVSTRHRTPFLFMTYYN
                     ILHRLGLRSFAEKTRAAGVQGVIVPDCPLEESAHCREALNERGIAPIPIFSPTSSDAR
                     LRQLGEVAQGLVYVVTRKGVTGASTDFSSELDAYLARCRAATKLPLAAGFGISSKAEV
                     DFFVGKVEIAVVGSQTLRVLDRSGVSGVSEFLKSLR"
     gene            complement(3016..3207)
                     /locus_tag="VFQ61_03790"
     CDS             complement(3016..3207)
                     /locus_tag="VFQ61_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953597.1"
                     /translation="MKTPLDDQLRAEIARFRLVFTCETCAHFEPDSQRCASEYPNAAH
                     RLRVLEDRELEFCKEYELS"
     gene            complement(3227..3913)
                     /locus_tag="VFQ61_03795"
     CDS             complement(3227..3913)
                     /locus_tag="VFQ61_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953598.1"
                     /translation="MSISSRSCGRTTSPGSVLGRAHRAAALLLVGALAAACTVGEGQG
                     FVSSKKLHVDNCWDGPFDLYPDFFAANPDSRNALAIRVQRGDNIAEQSDGLHVLVTEL
                     QDLRARLEEPLAVGLPRGVSPPGVPIQYQSDPPKVSLALYLHNSCHAEAATVYSLSGQ
                     ITFHALFSGNPNEGNAENRLTSAEFTDVVFGDPRRVTGNAEQDAASQSLVSGKFSFYF
                     QRGQPAQPFP"
     gene            complement(3935..5095)
                     /gene="mnmA"
                     /locus_tag="VFQ61_03800"
     CDS             complement(3935..5095)
                     /gene="mnmA"
                     /locus_tag="VFQ61_03800"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET9953599.1"
                     /translation="MHPDESCSCKAGSGHPLSGCATTRAFPMAPRVLLAMSGGVDSSV
                     AAARLIENGFDVVGATLHLWDYPEEGEAKSRCCAPEDVHDARRVADVLGFPHYAFDRR
                     ELFAREVVQPFVDSYLEGETPSPCVRCNRGVKLKELFGLADRLEAQFVATGHYARVRN
                     VDGRYELLRSLDRNKDQSYFLHMLDQARLSRLMFPVGELDKQSVRERALKLALPGASK
                     GESQELCFVSSGRYDRFVTERAEQRVRPGPILDESGKVVGNHAGIHAFTIGQRRNLGV
                     ALGERAYVVAIDAAQGRVQLGPKEALEMRGARLEELSLAAGVELPLRCEVQVRYRGQP
                     YPASVFMAAEGARVAFDGPVRAVAKGQYAVFYDGERVLGGGLIREALASAGD"
     gene            complement(5096..5785)
                     /locus_tag="VFQ61_03805"
     CDS             complement(5096..5785)
                     /locus_tag="VFQ61_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9953600.1"
                     /translation="MRDEAQARRLFEHSLELVEATAKQLGKLIGPSVELEELRSLGRL
                     GLLEAAQRYDESRDVPFRVFAKYRVRGAIIDGLRRMATLPRRVHRRLLALQFANQVSG
                     SSIQLMHGPDGKSAAEADQLVSRHLAAMAAAMADGLLAKTGQEDGEPVAVCSAANPEE
                     AYERHELWDSVRRAISELPAVEATLLARHYLHGEHFEHVAADLGMSKSWASRLHARAV
                     SRLSRRLRSAE"
     gene            6048..6650
                     /locus_tag="VFQ61_03810"
     CDS             6048..6650
                     /locus_tag="VFQ61_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9953601.1"
                     /translation="MPRWSLLALAYASVAALALSLAEFVFERSAFTYATPWLKLPGVM
                     THVYSLALGVALGALVVFATRKLVDRAAWARELARALRPFARGLSGTGILLLALFSSL
                     GEELLFRGLLQPSLGLWFQALLFGVLHQVSGPSRWAWVTWAGLVGLLFGMIFAATGSL
                     LGPIAAHALINGLNLNFLQNHDPELSRRGLGGLLGQRSRA"
     gene            7046..>8256
                     /locus_tag="VFQ61_03815"
     CDS             7046..>8256
                     /locus_tag="VFQ61_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9953602.1"
                     /translation="MSALEPGNSDEKPRVLVVDDEKFIRDIIADFLALEGYVVRTAED
                     GSSALGELSRAPYDMVISDLKMPNMGGLDLLKEVHRAYPNTLTVIMTGFGTVETAIDA
                     MKRGAYDYILKPFKVEDVVRVIQRGLEKSKLAAENLRLREALSLYQVTEAIAESLSLD
                     EVVNTLGESAQREVRADVVTVHLSDGEGGLLPRYRAVSTELQRDDEDVGELAVEKLRE
                     RLASGLPVLEQAGRAREFFERHPSRHVSSICSVPLKMRDRLLGFLSVVSLTQSQRFDE
                     GQRKLLSIIASRAAAAIENARLYADLQATFQQTIQGLAQTIDKMDRYTAGHSERVANY
                     AVLLARWLRLPEPMIEIVRHAALMHDIGKVGCVMNLNKPGKLTQKEYEVFKRHPVHGR
                     DILDPIKFLAPV"
BASE COUNT         1515 a         2557 c         2716 g         1468 t
ORIGIN      
        1 gtagttacgc ggggggccgg cgcggtgcag agacgcgaag agcatgcatt tgactgcgct
       61 cgggtgaaag aaggacgggc ggcgcgcccg tagcagagtg gcccaatcga ccgatttcat
      121 gggtcgacgg agcggcgatg cctgacccag attggacaag ttgtcttgac aacttggggg
      181 tccccgcgca tgctctgcgc gtgctctcca atggagcttt gcccctgtac gctcagatcg
      241 cgcgacgact gcgtgcgcag atccaagcgg ggcagcacgc gcgaggcgct cggatcccgt
      301 cggagcacga tctcgcggct cagttcgcgg tcgggcgccc cacggtgcgc caggcaaccg
      361 agctgttggt gcaagagggc gtgctcgagc ggcggcgtgg ctcgggcact ttcgtgagcg
      421 agctgcccaa ggaagtggac gtgttttcgg cgttcggcac gctggcgtcg ctggcgcaga
      481 gcgagctgcc cgtgagcgtc aggttactcg gtaaggtgcg aaagatcgct ccacccgaag
      541 cggcgagagc gcggctcaaa gagggccggg cgttcttcgt gcagcgcctg tgcagcttgg
      601 cgggcaagcc gctcttgctc gaagagatgt acttcgagcc cgaggttttc ccgggtttgg
      661 acgtgatgcc gctgaaagga cgctcgctgt cggagctctc ccgcgagcac tttcagctgc
      721 gaccggtcag cgccgagcaa cgcttcagct tgtttgcact ggatgaaaag cgcgcgcagt
      781 ggttggggct accggtcggg cgtgaggtgc tcaaggtcga gcgttgtctc gattttgcag
      841 gcgcgccccg cgcccactac gccgagctat tctgccgcac cgatcaagtt tgtttcactc
      901 agcacctgag tttcggtgca acgtcgaagg cgagtacgct gccggaccga ggcagaaccc
      961 aggccggagc cagcaggtaa ggatctctca cccatgacac tcgaccccgt gtacttctcc
     1021 gaatttggcg gcactttcat gccggagatc ttggtctcca ccttcgaaga tttgacgcgc
     1081 atctatcaag aggcgcgccg cgatccgaag ttcttcgcgg agtacgagca gctgatgcgt
     1141 tcgtattctt gccggccgac gccgatcacg ttcgctgaaa atctcacccg ccatttcggc
     1201 ggcgcgcgca tctacgtcaa gcgcgaggac ctgaaccaca ccggcgcgca caagctcaac
     1261 aacgtgatgg gccaaggctt gctcgtgcgg cgcatgggta aaaagcgcgt gatcgcggag
     1321 accggggccg gccagcacgg cgtcgcgacg gccacgatgg cggcgcgcct ggggctcgag
     1381 tgcaccatct atatgggtga agacgacgtg cggcgccaac gcccgaacgt gttctggatg
     1441 gagcggctcg gcgccacggt ggtgagcgtc aaggacggct cccgcacgct gaaagacgcc
     1501 atcaacgagg cctttcgcga ttgggtcgcg aacatggaca acacgcacta tgtgctcggc
     1561 acggcctgcg gtccccatcc gttccccgag atggtgagct ggttccagtc gatcatcggc
     1621 aaggaagctc gtgagcagat gctggcgatc gagggccgtc taccgcacca cgtctatgca
     1681 tgcgtgggcg gtgggtctaa tgccatgggg ttattcagcg gcttcatgga ggacaaggac
     1741 gtggagctcg tgggtgtcga agcgggtggg cacggcctgg agacgggcaa acatgcggcc
     1801 cgcctatccg gaacggatgc ttcaatcggg gtcgcccaag gctaccgttc gtacttcctg
     1861 caagacgaag acgggcagat gctggacacg cacagcgtgg cggcggggct cgactacgtg
     1921 ggcgtgtcgc cgattctggc gcacctcaag cacatcgggc gggcgcgttt cgaggtcgcg
     1981 acggatcaac aagccatcga tgcgctcgcg ctcgccgctc gcaaagaagg cctgattccg
     2041 gcgctggagt cctcgcatgc tttcgcgtgc gcgttccgcg aggcgcccct ccacaaagac
     2101 caaatcattc tgatcaatca atccgggcgc ggcgacaaag acatctttac cgtcgctgac
     2161 gccttcggcg atccggcttg gaaggaattc atccggagaa aggcctcgga atatgcagct
     2221 gagtgagtac atccgcgcgc agcgcgagaa gcaacgtatc ctgctcatga cccacatcgt
     2281 ggtgggttac ccgaacctcg agcgcagccg cgagctggtg gacgcgatgg tggccgcggg
     2341 cgtggacctg atcgagctgc agattccgtt ctcggagccg gtggcggatg ggcccgtgat
     2401 tctgagggcg aaccaagaag ccttgaaggc gggcacgcga gtcgcggact gcctcgagtt
     2461 cgcgcgtgac gtctctacac gtcaccgcac gccgttcctg ttcatgacct actacaacat
     2521 cctgcaccgg ctcggcctgc gcagcttcgc ggagaagaca cgcgcggccg gagtgcaggg
     2581 cgtgattgtg cccgattgcc cgctcgagga gagcgcgcac tgccgcgaag ccttgaacga
     2641 gcgcggaatt gcgccgatcc caatcttctc tccgacgagt tcggatgcgc gtctgcgcca
     2701 gctgggagag gtggcccaag gcttggttta cgtggtgaca cgcaaagggg tgacgggcgc
     2761 gtcgacggac ttcagcagcg agctcgacgc ctacctcgca cgttgccgcg cggcgacgaa
     2821 gctgccgctc gcggccggct tcggaatcag cagcaaagcc gaggtggact tcttcgtcgg
     2881 caaagtcgag atcgcggtgg tgggcagcca aaccttgaga gtgctcgatc gcagcggtgt
     2941 ttccggcgtt tcggagttct tgaaatcgct ccgctgaagg ctggcacgtc tcgctgacgg
     3001 acttgggagg ttgagctagc tcagctcgta ctctttacaa aactcgagct cccgatcttc
     3061 cagcacgcgt aggcggtgcg ccgcgttggg gtactcgctg gcgcagcgct gggagtcggg
     3121 ttcgaagtgc gcacaggtct cgcacgtgaa aaccagccga aatcgtgcga tttcggcgcg
     3181 gagctggtcg tcgagtggcg tcttcatgga tcgcgcggca gccgcattac gggaagggct
     3241 gcgcgggctg accgcgctgg aagtagaagc tgaatttccc gctgaccaag ctttgcgagg
     3301 ccgcatcctg ctcggcgttt ccagtcacgc gccgcgggtc cccgaacacg acgtccgtga
     3361 actcggccga cgtcagccgg ttttcggcgt tgccctcgtt gggattgccg ctgaacaggg
     3421 cgtgaaaggt gatctgcccg gacaacgagt aaaccgtcgc ggcttcggcg tggcagctgt
     3481 tatgcaggta cagagccaag ctgaccttgg gtggatccga ctggtattgg atgggaacgc
     3541 cgggggggct gactccgcgc ggcagcccga ccgcgagcgg ctcttcgagg cgagccctca
     3601 agtcttgcag ctccgtcacg agcacgtgca agccgtccga ttgctcggcg atgttgtccc
     3661 cgcgttgaac gcgaatggcg agggcattgc ggctatccgg attggcggcg aagaagtcgg
     3721 gatagagatc gaacgggccg tcccaacagt tatcgacgtg gagcttcttg ctggagacga
     3781 agccctgccc ttcgcccacc gtgcacgccg ccgctagtgc tccgaccagg agcagagcag
     3841 cggctcgatg cgcccgaccg agtacgctac ccgggctagt ggttcgccca cagctgcggc
     3901 tcgaaatgct catttcgcca gcccttcatg cagttcaatc tcccgcagag gctagcgcct
     3961 cccgaatcaa gcctccaccg agaacgcgct ctccgtcata gaagacggca tattgcccct
     4021 tcgccactgc gcgcaccggg ccgtcgaagg cgacgcgtgc tccctccgca gccatgaaca
     4081 ccgaggccgg atagggttgg ccgcggtaac ggacctgaac ttcgcagcgc agcggcagct
     4141 cgacccctgc ggccaaagac agttcttcca agcgcgcccc ccgcatttcc agagcctctt
     4201 tcgggcctag ctgcacgcgg ccctgggcgg catcgatggc gaccacataa gcgcgttcgc
     4261 cgagagcgac gccaaggtta cggcgctgcc caatggtgaa cgcatgaatg cctgcgtgat
     4321 ttcccacgac cttgcccgac tcgtcgagga ttgggcccgg acgcacgcgc tgctccgcgc
     4381 gctcggttac gaagcggtcg tagcggccgc tcgaaacgaa acacagctct tggctctcgc
     4441 ctttgctggc cccgggtagc gcgagcttga gcgcacgctc tcgcacgctc tgcttgtcga
     4501 gctcgcccac tggaaacatc aggcgagaca gacgagcctg atccagcatg tgcaagaaat
     4561 agctttgatc cttgttacga tcgagcgaac gcagcagctc gtaacggccg tccacgttgc
     4621 gtacgcgggc gtaatgccca gtcgcaacga actgcgcttc caggcgatcg gcgagcccaa
     4681 acagctcttt tagcttcacg ccgcggttgc aacgcacaca gggactgggg gtctcccctt
     4741 cgaggtacga gtccacgaag ggctgcacga cctcacgcgc gaacagctcc cggcgatcga
     4801 aggcatagtg cgggaagccc agcacgtccg cgacgcgacg cgcatcgtgc acgtcttccg
     4861 gcgcgcaaca gcggctcttc gcttcgccct cttcggggta atcccagaga tgcaacgtgg
     4921 cgccgaccac gtcgaagcca ttctcgatca gacgcgccgc ggcgacggaa gaatcgacgc
     4981 cgccgctcat ggcgaggagt acgcgaggag ccattgggaa ggcgcgagtg gtagcgcatc
     5041 cactgagcgg atgcccgctt cccgctttgc acgagcagct ttcgtccgga tgcatttact
     5101 cggcgctgcg caatcggcgg gataacctgc ttacggcgcg cgcgtgcagc cggctcgccc
     5161 aggacttgct catgccgaga tcggcggcaa cgtgctcgaa gtgctccccg tgcaggtagt
     5221 gccgggcgag tagcgtggct tccacggcgg gcagctcgct tattgcgcga cgcaccgagt
     5281 cccaaagctc gtggcgctcg taagcttcct cgggattcgc cgcagagcag accgccaccg
     5341 gctcaccgtc ttcctgccct gtcttggcca gaaggccgtc ggccatcgcg gccgccatcg
     5401 ccgccaaatg ccgagagacg agctggtcgg cttcggcggc agacttgccg tcaggcccat
     5461 gcatgagctg aatcgagctg ccggaaacct ggttggcgaa ctgcaacgcc agcaatcgcc
     5521 gatgcactct gcgaggcaga gttgccatgc gccggagtcc gtcgatgatc gcaccgcgca
     5581 cgcgatattt ggcaaagact cgaaatggga catcgcgcga ctcgtcgtag cgctgcgccg
     5641 cctcgagcag cccgagcctg cccagactac gcagctcctc gagctcgacg ctgggtccaa
     5701 tcaacttgcc caactgtttc gccgtcgctt ccacgagctc caaagaatgc tcgaagagcc
     5761 gacgcgcctg cgcctcgtcc cgcacacaca acctccgcgg gacaatctat ccgaaacggg
     5821 gctatgtcgc gaaatagcaa tgtgacgtac aagcgccacg ctctcgcgcg ccaacggcgc
     5881 ggaaacctgc acacccggcg actgtccgat cacctcaggg gcagtgcccg catggctgcg
     5941 cgtcggtgtc gtcggaccgt gccgacactc gcgggccggc cactgaacgt cgagggatcg
     6001 cgcggcgagc tcccgaacca gccctggctg tggcatagtc cacggcgatg ccccgctgga
     6061 gcttgctcgc gctcgcgtac gccagcgtcg ccgctctggc gctgagcctc gcggagttcg
     6121 tgttcgagcg ctcggcgttc acgtacgcga cgccgtggct gaagctgccc ggcgtgatga
     6181 cgcacgtgta tagcttggcg ctgggggtgg cgctgggagc gctcgtcgtg ttcgcgactc
     6241 gcaagctagt cgatcgcgcg gcttgggcga gagagctagc gcgcgcgctg cgcccctttg
     6301 cgcgtgggct cagcggcacc ggcatcttgc tgttggctct gttcagcagc ttgggcgagg
     6361 agttgctatt ccgaggcttg ctccagccga gcttgggtct gtggtttcag gccttgttgt
     6421 tcggggtatt gcatcaggtg agcggcccga gtcgctgggc atgggtcact tgggcagggc
     6481 tggttgggct tttgttcggg atgatcttcg cggcaaccgg gtcgctgctc ggtcccatag
     6541 ccgcgcatgc gttgatcaac gggttgaacc tcaatttcct gcaaaatcac gatccagagc
     6601 tgtctcggcg cgggctcggt ggcctcctgg ggcagcgcag tcgggcctga gcggcagatg
     6661 ccgggagcgg cacggctgaa cacaacgccc cggccggctc caggcgagct aagccggctc
     6721 cagacgagct aagccgggtc acgagcggca gtgctggagg cgcgcgccaa aaataagagc
     6781 tcgaacgtgg gcccggacgc cgcggctcgc gacccatccg ccgtttcgac cgagaattcg
     6841 tgcttacttc gcccagcgga gtgaccgaag cccttctgtc gagcaaaaac tctggagttt
     6901 caggctcaaa ttggggttcg gaggcgtatt accgggagca caggaatgcg aagtgccagc
     6961 atggccgtgg atttgctgcg gcgcaccgca cgaaaagctg cgcagggacc cgaaacagcg
     7021 ctaagaattg gccagcctct cgcccatgtc cgcgctcgaa ccgggaaata gcgacgagaa
     7081 acctcgtgtg ctcgtcgtcg atgacgagaa attcatccgc gacatcatcg ctgatttcct
     7141 ggcgctcgag ggctacgtag tgcgcacggc cgaggatggc tcgagcgcac tcggcgagtt
     7201 gtcccgcgcg ccgtacgata tggtgatcag cgatctgaag atgcccaaca tgggtgggct
     7261 cgatttgctg aaagaggtcc atcgcgcgta tccgaacacg ctcaccgtga tcatgaccgg
     7321 gttcggcacg gtggagacgg cgatcgacgc gatgaaacgc ggcgcttacg actacatctt
     7381 gaagccgttc aaggtggaag acgtcgtgcg agtgatccag cgcggtctgg agaagagcaa
     7441 attggcggcc gagaacttga ggctgcgaga ggcgctctca ctttaccaag tcaccgaagc
     7501 cattgccgag agcttgtcgc tggacgaagt cgtgaatacg ctcggggaga gcgcgcagcg
     7561 ggaagtgcgc gcagacgtgg tcacggtcca cctctcggac ggagaaggcg ggctcttacc
     7621 cagataccgg gcggtctcga ccgagttaca gcgagacgac gaagacgttg gggagctggc
     7681 ggtcgagaag ctgcgggaac gcttggcgag cgggctcccc gtactcgagc aggccggccg
     7741 cgcgcgagag ttcttcgagc gccacccgag ccggcacgtg agcagcatct gctcggtacc
     7801 gctcaagatg cgggatcggc tgcttggatt tttgtccgtg gtgtcactca cgcagagcca
     7861 gcgtttcgac gaaggccaac gcaagctgct gagcatcatc gcctcgcggg ctgcggccgc
     7921 gatcgagaac gcgcgcttgt acgcggacct gcaggcaacc ttccagcaaa cgatccaagg
     7981 cctggcccaa accatcgaca agatggatcg ctacaccgcc gggcactcgg aacgcgtggc
     8041 aaactacgcg gtactgctcg cgcgctggtt gcgtttaccc gagccgatga tcgagatcgt
     8101 gcgccacgcg gcgttgatgc acgacatcgg taaggtgggc tgcgtgatga atttgaacaa
     8161 gcccgggaag ctgacgcaaa aggaatacga ggtcttcaag cgtcatccgg tgcatggccg
     8221 cgacatcttg gacccgatca aattcttggc tccggt
//
LOCUS       DASTKU010000108        11523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1135766, whole genome shotgun sequence.
ACCESSION   DASTKU010000108 DASTKU010000000
VERSION     DASTKU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11523
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135766"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..3369
                     /locus_tag="VFQ61_03820"
     CDS             148..3369
                     /locus_tag="VFQ61_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953603.1"
                     /translation="MSRRSFGAVFKLVGALSTALIAAGCSSVAPQESPDETASLMEEL
                     GESLTSCVAADGTAWANGVLTIDATTNTSGSVIVSPGNSIVKVNGRTCKTAAGAVIAL
                     TAVKQIIVNGKAGSTDTFVLDFSAGLPKAPLLQASGIIVNGNETIASTTTPDTVVFKG
                     STSADPVSVGLDATNTAEGQVDIALTATTKLANIVVKNVGRSVVGPYQPQLIFSLGTG
                     DDTFTGLGGFPTGSASTAMNYGMAIYGGAGNDTLIGGLGSDKIIGGGQATDTLDYSGR
                     TTTAVYADMDSTKVTVWGGDLRGVTMANETIKIDKGGGSLTTVTFATEATPKDIVATI
                     NSALSATVATLAGNRLRLVSTSGNIRIDGNNSMAVNKLGLAVGLYSALGNDGTPGGQM
                     DLPWKAPTARADSTVYKVGDVAVPAAANGFWYRVSVAGTSGVGAPTWDTTIGGTTAAD
                     GGVTWVTAGKVWKAATAYKPGDVVYDVANNAIYKIATLTGKTGGSGPTLATSLNGTVT
                     DGGITWTTVGISWAATTAFTTGKLAIPATPNGYYYKATTGGTTGGSAPTWPTTVGATV
                     VDGSVTWTNMGTAPAIPQSTLLAVGTYGYDAAGYLAQATASALTSGGSIAWPSPAADK
                     TDNDITWTYIGGRAEADDVQGISVLSGGGVADVLIGNNLANTLNGNAGNDVLMGGPLM
                     TPSANCPVDKLNGGDGNDWFDMGPDDAVNPLTQNDCTQVVAGGLGTDVVDYSRRTSNG
                     NVLAKLDGTTPSGEQTLSPKEADKLGNDIEILLGGGGDDTLIGTDNTDYLFGGAGADK
                     IYGGKGDDHIYGGAGDDKLYGEVGNDFFYELTAYPAATAWNEPLAGGTFAGVGLSIFG
                     AEMPGAGDDTVLGGADMTETNKIDFTDAANAVSVALCADAAATTTDLKSCVSSKSLYV
                     ANSSDGTSGGGAGTANTYVNVQYLAGALTKANTFIGTSYAEVFEGGKAVDIMLGQGGQ
                     DTLFGYADAAAGGAVDNSSADVLCGGDDDDTVYAGTAATIEGEGQMDQAFANSGTTSV
                     LLGGASAQAPSYCTNWSASTNPYMSVEGVNVCFGSTTKYHCAN"
     gene            3519..4808
                     /locus_tag="VFQ61_03825"
     CDS             3519..4808
                     /locus_tag="VFQ61_03825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9953604.1"
                     /translation="MKVVHLTTSAGRAGGGAYASAYRIHASLRELGVASEMLCSIRRV
                     DDPSVTELPRKGAAGWRRRLAIRLDGTPLRLFRPKPHMPFNNGFVGALAAARHPLVQS
                     ADVLQLYWVAAGFLSVRSLGSLLGLGKPVVWRLSDMWAFTGGCHYPLECQRYREHCGH
                     CPELERPFAYDISRLNFELKRAAYGAGAFTIVTPSRFLASACRQSALLGHARIEQIPT
                     GIDVNAFCPRDRVRARARLGLPASGRVVLFGARGGLSDPRKGGRFAVEAAQYIAKTER
                     GGAAPPVCFAVFGRKELVLEGVTSSVNLGLLDDEQSLADAYSAADVFLAPSLQDNLPN
                     TVLEAIACGCPVVGFRDAGLDDAIRDGQSGFLVPRGDAVALGEACRRVLEDADLAAHL
                     RESARLLAEHEFDQDLQAQRFLSLYRELLDQRSLKPA"
     gene            complement(4858..5649)
                     /locus_tag="VFQ61_03830"
     CDS             complement(4858..5649)
                     /locus_tag="VFQ61_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953605.1"
                     /translation="MLTHAQDALVWTERLMAACILQQTLELWLIRRQLRDDGVFAFPV
                     LRREYRLFPAPLRLLCDWGLNYPAFTALLGLRVAGAWGLALGVSEFAVPLWLSQVAVC
                     VRFRGTFNGGSDYMTVQVLLALGTATVLRSSVALTGGLFYIAVQSTFSYFIAGVAKAK
                     EADFWSGRAVLGFVKLPQYAAPSWAKRVLSIPPVRRISALAVLLFECGFPLAWMGPKL
                     ALAWLATGATFHLVNFFVFGLNRFFFVWLATYPALLFSVQKVAAG"
     gene            complement(5643..6188)
                     /locus_tag="VFQ61_03835"
     CDS             complement(5643..6188)
                     /locus_tag="VFQ61_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953606.1"
                     /translation="MLIAAALLVGLGVLVLFVLRERTTLASPAWMLLRCLLPAYRFFE
                     RVDPLPALNVRFGTTELGPWFLALEPPARRGFHLFLNPAGNLYLAHCSLVERFLDDLT
                     DADPEQVPQLVSYELLKRLAARAACARLGDDPTQAGVHSSSLAPRFQFRLVERDAAPV
                     EPSAAYEASQLFVSLTHELTC"
     gene            6445..7272
                     /locus_tag="VFQ61_03840"
     CDS             6445..7272
                     /locus_tag="VFQ61_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953607.1"
                     /translation="MATDDAQTRRSRDEEGRGAAPRKRKKGAKSAKLSRPLTEKEINV
                     PDLQTLIMMGALGITALVMWAFARSGCNYHPPRETRVPRKVTTAELTREPRDAAIEFQ
                     HRLLTYDFAGAAEIAAGSAVQDVEREKIACGPDCAKKKAALVDPITSAVIVERTPFNA
                     KVKVTTYRIPGGDKTFLNLAERSPGGWKITARVPDAPGATLPPPSMSPPDGHFGMTPN
                     GPRFPITPPGASSAAPAGSAAAAPSSSAPAPTGNAGPAASAVKPKTVAPAAPAPSAR"
     gene            complement(7284..7793)
                     /locus_tag="VFQ61_03845"
     CDS             complement(7284..7793)
                     /locus_tag="VFQ61_03845"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9953608.1"
                     /translation="MNARLSRLLDYALYGALGVTLVTFVSRKMSGPTEGKTAAPIDLP
                     LLGEEKRFKLAEHANRPVLLEVFASWCGVCERTAPSVGAAFAKHSKSGVTFLGVSVDS
                     NPADAARAKQSWPIPYDVALDDGSLSRAYDIKVLPTFVLIGADGRVKHVSTGAASEST
                     LESWLSDAR"
     gene            complement(7790..8497)
                     /locus_tag="VFQ61_03850"
     CDS             complement(7790..8497)
                     /locus_tag="VFQ61_03850"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HET9953609.1"
                     /translation="MKPGADIDPLADLPWPTPKAPSGEVSAAICGQCTHKLKPQRGLK
                     SWQRLVLSVILSLSVFGTLAWLTRGRARFEGTFHNALVGAAGWGIVLAAVMWAGLARP
                     PGRRVSTRIRLVMAVLLPVGFLMYVVHAAPEWVSFAEFSHGARAAHAIGCSLVGLVFS
                     AIVSGGVLLLWRGTDPLSPSLSGALVGLVGGVGGSLAIGVGCASQEGWHSSFAHGLGV
                     IAFTLLGWGVGRRLLSP"
     gene            complement(8494..9282)
                     /locus_tag="VFQ61_03855"
     CDS             complement(8494..9282)
                     /locus_tag="VFQ61_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9953610.1"
                     /translation="MTAGSISPPIGIQLAEVDFLRRSRASKPGASQAGMGGASQAGAS
                     QPSLGPAGVGDAAVLPTLAREQAPRGEAQERERTEGNPLRARLDGLMERYVDGDPSAF
                     EELYRGVSPTLFGYLLRLTRDRHRAEDLLQITFSKVHRARGSYLRGAPVLPWLLAIAR
                     RSFLDERRHAQTRTEDLSPDGNLPEPHSEERAVSTDLSEALERALDGLPEAYREAIQL
                     TKISGLSLTDAASVLNTTETAVKLRVHRGYEQLRKQLERFNRNA"
     gene            complement(9353..10201)
                     /locus_tag="VFQ61_03860"
     CDS             complement(9353..10201)
                     /locus_tag="VFQ61_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9953611.1"
                     /translation="MRKLSRLSGLAGLLLLACGHSEDEWQAQLHKYQELVNQNQHEQQ
                     ARQQTQAELETAARQIVALKEQLQKMGVNLETLNQQLEQSGTQNKELSKNLEEMARAL
                     DEYKARAAQLERIRQRFEQLRDKLKKLTDLGLKVEIRRNRMVIRLPGDVLFASGEDKL
                     REQGEKVLDAVAEVIRNDRQLAGRYFQVAGHTDNKPLLGGRFGDNWGLSVMRARRVLL
                     YLIAPVDGKAKGGGLDPMRLHAAGYGETDPVQKNDSDESRQQNRRVELVLMPDVEEML
                     DLRSLI"
     gene            10403..11122
                     /locus_tag="VFQ61_03865"
     CDS             10403..11122
                     /locus_tag="VFQ61_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9953612.1"
                     /translation="MGLILVIEDEPDIRQVLDYNLRAAGHEVIEAESGTQGLDRVRTQ
                     RPDLVLLDLMLPDLPGTEVCRAIKEDPTTRGISVVMLTARGDEIDRVVGFEVGADDYV
                     TKPFSVRELLLRVRAILRRNEVKVVETNTVEFGTLRVDRDAHRVWVGDEEVELTALEF
                     KLLVTLYDRKNRVQSREVLLDTVWGIENKISTRTVDAHVKRLREKMGAARDYIETVRG
                     VGYRFAEAPVLDADCWDTRSA"
     gene            11247..>11523
                     /locus_tag="VFQ61_03870"
     CDS             11247..>11523
                     /locus_tag="VFQ61_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953613.1"
                     /translation="MQMLTHTVLLALLALTAISTLLTAVSHFSVSRVLRRQAAPPTRL
                     PAISVLKPLKGADEELYENLVSLARQDYPDFELLFGCEDPNDPALPVV"
BASE COUNT         2158 a         3665 c         3701 g         1999 t
ORIGIN      
        1 cgtcagcaac ccgacgcatt cgaggcatgg acgctaaagg ttcgaggctc ctgccgttat
       61 taacttgcgc agccaccgga ggcgaagcga gcgtcagtct gacgtgcttc tgccgggctc
      121 cgggaattca tgcgggagaa gattcagatg tcgagaagat cgttcggtgc cgtgttcaaa
      181 ttagtgggag ctttatcgac cgcactcatt gcggcagggt gtagttctgt cgcgccacaa
      241 gagtcaccgg acgagacggc ctctctgatg gaagaactcg gcgagtcgct cacgagctgc
      301 gtcgccgcag acgggacggc ttgggcgaac ggcgttctga ccatcgacgc cacgaccaac
      361 acctcgggtt cggtcatcgt ctctcccggc aattcgatcg tgaaagtgaa cggtcgtacg
      421 tgcaagacgg cggcgggcgc ggttatcgcg ctgaccgcgg tcaagcaaat catcgtcaac
      481 ggtaaggcgg gctcgaccga cacgttcgtg ttggacttct cggcgggctt gccgaaggcg
      541 cccttgctcc aggcgagcgg catcatcgtc aacggtaacg agaccatcgc ttccacgacc
      601 actccggaca cggtggtatt caagggcagc acttcggcgg acccggtcag cgtcggtctc
      661 gatgcgacga atacggctga aggccaagtc gacatcgcgc tcaccgcgac caccaagctc
      721 gcgaacatcg tggtcaagaa cgtggggcgc agcgtggtgg gtccctacca accgcagctg
      781 atcttcagct tgggcacggg tgacgatacc ttcaccggcc tcggcggctt ccccacgggt
      841 tcggcctcca cggccatgaa ctacggcatg gccatctacg gcggtgccgg caacgacacg
      901 ctgatcggcg gtctcggttc ggacaagatc atcggtggcg gccaagccac ggacacgctg
      961 gattacagcg gccgtactac cacggccgtc tacgccgaca tggacagcac caaggtgact
     1021 gtttggggcg gcgatttgcg tggcgtgacg atggccaacg agaccatcaa gatcgacaag
     1081 ggcggtggtt cgctgacgac ggtgaccttc gccaccgagg cgaccccgaa ggacatcgtc
     1141 gcgaccatca actccgcgct gtccgcgacg gtggccacgt tggccggcaa tcgcctgcgc
     1201 ctggtctcga cctcgggcaa cattcgcatc gacggcaaca acagtatggc cgtcaacaag
     1261 ctcggtctcg cggttggtct gtacagcgcc ctcggcaacg acggaacgcc cggcggccag
     1321 atggacttgc cgtggaaggc gcccaccgcg cgcgccgact ccacggtgta caaagtcggc
     1381 gacgtggccg tgcccgcggc ggccaacggc ttctggtacc gcgtgtccgt tgcgggaacc
     1441 tcgggcgtcg gcgcgccgac ctgggacacc acgattggtg gaacgaccgc cgcggatggt
     1501 ggcgtgacct gggttaccgc gggcaaggtt tggaaggcgg ccactgcgta caagcccggt
     1561 gacgtggtgt acgacgtggc gaacaacgcg atctacaaga tcgcgacgct caccggtaag
     1621 accggtggta gcggtccgac cctcgccacc tcgctgaacg gcaccgtcac ggacggcggc
     1681 atcacctgga ccacggtggg catttcgtgg gccgccacga ccgctttcac cacgggcaag
     1741 ctcgcaatcc ccgcgacgcc gaacggttac tactacaagg cgacgacggg cggcacgacc
     1801 ggcggctcgg cgccgacctg gcccacgacc gttggcgcga ccgtagtgga cggctccgtg
     1861 acctggacca acatgggcac ggctccggcc atcccccaga gcacgttgct cgcggtgggc
     1921 acttacggtt acgacgcggc cgggtacctc gcgcaagcca cggcctcggc gctgaccagc
     1981 ggcggtagca ttgcttggcc gagtcccgcg gcagacaaga ccgacaacga catcacctgg
     2041 acctatatcg gcggtcgcgc cgaagctgac gacgtgcagg gcatctcggt cctgagcggc
     2101 ggcggcgtcg cggacgtcct gatcggcaac aacctcgcca acacgttgaa cggcaacgcc
     2161 ggcaatgacg tgctgatggg tggaccgctc atgacgccga gcgcgaactg ccccgtcgac
     2221 aagctgaatg gtggcgacgg caacgactgg ttcgacatgg gtcccgacga cgcggtcaac
     2281 ccgctcaccc aaaacgactg cacgcaggtc gtggccggtg gtttgggtac ggacgtggtc
     2341 gactacagcc gccgtacttc gaacggtaac gtcctcgcga agctcgacgg caccacgccg
     2401 agcggcgagc agacgctgtc gccgaaggaa gcggacaagc tcggcaacga catcgagatt
     2461 ctgctcggcg gtggcggcga cgacaccctg attggcaccg acaacaccga ttacctgttc
     2521 ggtggcgcgg gtgccgacaa gatctacggc ggcaagggtg acgaccacat ctacggtggc
     2581 gccggcgacg acaagttgta cggcgaggtc ggaaacgact tcttctacga gctcacggcc
     2641 tatcctgcgg ctacggcttg gaacgagccc ctggccggtg gcaccttcgc cggtgtcggt
     2701 ctctcgatct tcggcgcgga aatgccgggt gcgggcgacg acacggtgct cggtggcgca
     2761 gacatgaccg aaaccaacaa gatcgacttc accgacgcgg ccaacgcggt gtccgtcgct
     2821 ctgtgcgccg acgccgcggc gacgaccacc gacctgaaga gctgcgtctc ctcgaagtcg
     2881 ctctacgttg ccaacagctc cgacggtacg tcgggcggcg gtgctggcac cgccaacacc
     2941 tacgtcaacg tgcagtacct cgccggtgcg ctgaccaagg ccaatacctt catcggaacc
     3001 tcgtacgccg aggtcttcga gggtggtaag gccgtggaca tcatgctcgg tcagggcggt
     3061 caagacaccc tgttcggcta cgccgatgcg gctgccggtg gcgcggtcga caactcctct
     3121 gcggacgtgc tctgcggcgg cgacgacgac gacacggttt acgcaggcac cgcggccacg
     3181 atcgagggtg aaggtcagat ggaccaagcc ttcgcgaact ccggtaccac ctcggtgttg
     3241 ctgggcggcg cgagcgcaca agccccgagc tattgcacca actggtcggc ttccaccaac
     3301 ccatacatga gcgtggaagg cgtgaacgtc tgcttcggtt cgacgaccaa gtatcactgc
     3361 gcgaactgag caccccgtgt gttcgcgggt cgaaagatcc gcgaaccgaa cgcgggccaa
     3421 ctagaaccta gccccgcatc cagaagagcg catcgatgga gaccccgtcg atgcgctctt
     3481 tgcattttgc ccagtgctca agtacacact cgtgcgtgat gaaagtggtg cacctcacga
     3541 cttcggcagg tcgtgcaggc ggaggcgctt acgcctctgc gtatcggatt catgcctcgc
     3601 tgcgcgagct cggtgtggcg agcgagatgc tgtgctccat tcggcgcgtc gacgacccga
     3661 gcgtcactga actgcctcgc aaaggcgccg cgggttggcg tcgccggttg gcgattcgac
     3721 tggacggcac cccgctgcgg ctgtttcgac cgaagcccca catgccgttc aacaatggct
     3781 ttgtcggagc gcttgccgcg gctcggcacc cgttggtgca gtcggcagat gtgctgcaac
     3841 tctattgggt ggcggccggc ttcttgtcgg tgcgtagtct cgggagtcta cttggtcttg
     3901 gtaagccagt ggtttggagg ctttccgata tgtgggcctt cacgggaggc tgtcactatc
     3961 cgctcgagtg ccagcgctat cgcgaacact gtggccattg tccggagctc gaaagacctt
     4021 ttgcttacga tatatctcgg ctgaacttcg aactgaaaag agctgcatac ggtgccggcg
     4081 cgttcaccat tgtaactccg agtcggtttc tggcctcggc gtgccgacag agcgcgttac
     4141 tggggcatgc tcgcatcgag cagatcccaa ctggcatcga tgtgaacgcg ttctgcccga
     4201 gagatcgcgt ccgggcgcgc gcgcgcctcg ggctacccgc aagcggccga gtcgtgctgt
     4261 tcggtgcccg tggtggcctg tccgacccgc gcaaaggtgg gcgtttcgcg gtggaagctg
     4321 cgcaatacat cgcgaagacc gagcgagggg gggcggctcc gcccgtgtgt tttgccgtgt
     4381 tcggccgcaa agaactggtt ttggagggag tgacgagcag cgtgaacctg ggcctcctgg
     4441 acgatgaaca gagcttggcc gacgcctact cggccgcaga cgtgttcctt gcgccttctc
     4501 tgcaagacaa tctgcccaac acggtgctcg aggccatcgc ttgtggctgc ccggtggtgg
     4561 gcttccgaga tgcgggtctg gatgacgcta tccgcgacgg gcagagcggg ttcctcgtac
     4621 cacgagggga cgcggtggct ctcggcgagg catgccgacg agtgctcgaa gacgcagact
     4681 tggcggcgca cttgcgcgaa agcgcgcggc tgctcgcgga acacgagttc gaccaggact
     4741 tacaagcgca gcgcttcctc agcttgtatc gtgaactgtt ggaccaaagg tcgttgaagc
     4801 cggcttgagt tagagagctg gcgatacgcg acgggaacgg gcggaacgtg ctcaaggcta
     4861 ccctgcggcg accttctgaa cggagaataa caaagccgga taggtcgcga gccacacgaa
     4921 gaagaagcgg ttgagaccaa agacgaagaa gttgacgagg tggaaggttg ctcccgtggc
     4981 gagccaggcg agcgcgagct tgggacccat ccaggcgagt gggaagccgc actcgaagag
     5041 cagcaccgca agcgcactga tgcgccggac gggaggaatc gagagcactc gcttggccca
     5101 gctgggcgcc gcgtactgtg gcagtttcac gaagccgagc acggctcgcc cactccagaa
     5161 gtcggcttct ttggccttcg ccacgcccgc gatgaagtag gaaaaggtgc tttggacggc
     5221 gatgtagaag aggccgcccg ttagcgcgac ggatgaacgc agtaccgtgg ccgtgccgag
     5281 cgccagcagc acttgcacgg tcatgtagtc gctgccgccg ttgaaggtgc cgcggaaccg
     5341 cacgcaaacc gcaacttggc tcagccagag cggcactgcg aattccgaga cgcccaaagc
     5401 caaaccccag gcgccggcca ctcgcagtcc gagcagagcc gtgaacgccg gatagttcag
     5461 gccccagtcg cacaacaggc gaagcggtgc gggaaagagt cgatattcac ggcgcaacac
     5521 cgggaaggcg aacaccccgt catcgcggag ctgtctccgg atcagccaca actctagcgt
     5581 ctgttgaagg atgcatgcgg ccatcaggcg ctcggtccaa acaagcgcgt cttgcgcgtg
     5641 cgtcagcatg tgagttcgtg cgtcaacgag acgaacagtt ggctagcttc gtatgcggcg
     5701 ctcggttcga ccggcgctgc gtctcgttcg accaaacgga actgaaagcg cggcgcgagg
     5761 gaactcgaat gaacgccagc ttgggtcggg tcatctccga ggcgggcgca tgccgcgcgc
     5821 gcggccaggc gcttcaagag ctcgtacgag acgagctgcg gaacttgctc gggatccgca
     5881 tccgtgaggt cgtcgaggaa ccgctctacc agcgagcaat gtgcgaggta taggttaccc
     5941 gccgggttga ggaacaagtg gaacccgcgt cgggccggtg gttccagcgc gaggaaccag
     6001 gggccgagtt cggtagtacc gaagcggacg ttcagtgctg gaagcgggtc cactcgttcg
     6061 aagaaccgat aggcgggcag caggcagcgc agcagcatcc aggccggtga ggccagcgtc
     6121 gtacgctccc gcagcacgaa gagcacgagc acgcccaagc caaccagtag ggccgccgcg
     6181 atcaacatgg gtgttccctc tctcgcctcg actcggccat ctgccgccct cttccaggtg
     6241 cgaaccgacg ctctattctg cgcgcaatac tgtgctcgag gcgttcaatt gcacggctaa
     6301 tctgtttggg aaaacatgcg gtgtaaatgc tcggccgaaa ggcctgagcc tctgctcgcc
     6361 gtctcggctc ggtcgcgtaa ccggctcctc cacaatggat tctggggttg cgtcgaggtc
     6421 gactcgaagg aatgcttcgc cgccatggcg acggacgatg cgcagaccag aagaagtcgg
     6481 gacgaggagg gcagaggggc ggccccacgc aagcggaaga aaggggccaa gtcggccaag
     6541 ctgtcgcgtc cgttgaccga gaaggagatc aacgttcctg accttcaaac gttgattatg
     6601 atgggtgcgc tcggcatcac ggcgctcgtg atgtgggcct ttgctcgcag cggctgcaat
     6661 tatcacccgc cgcgcgagac gcgtgtgccc cgcaaggtga cgacggcgga gctgactcga
     6721 gagccccgcg acgccgccat cgagttccag caccgattgc tcacctatga cttcgcaggc
     6781 gcggcggaga tcgcggccgg cagcgccgtc caggacgtcg agcgcgaaaa gatcgcctgt
     6841 ggtcccgatt gcgcgaaaaa gaaggcggcg ctggtcgacc cgatcacgtc tgcagtaatc
     6901 gtggagcgaa cccccttcaa cgcgaaggtc aaagtgacca cgtatcgcat ccccggtggc
     6961 gataagacat tcctgaatct ggcagagcgc agcccgggtg ggtggaagat cacggcgcgc
     7021 gtgccggacg cgccgggggc caccctgccg cctcccagca tgtcaccgcc ggacgggcac
     7081 ttcggcatga ctcccaacgg acctcgcttc ccgatcaccc cccccggtgc gagcagtgcc
     7141 gctccggcag gtagcgccgc ggcagcgccg agcagtagcg cgcctgcccc gacgggcaat
     7201 gccggtcccg cagcgtccgc ggtaaagccc aagacggtgg cgccggcagc tcctgccccg
     7261 agcgcacgct agtccgaagc cagtcagcga gcgtcggaga gccagctctc gagtgtgctc
     7321 tccgacgcag cgccggtaga cacatgcttg acgcggccat ccgcgccaat cagcacgaac
     7381 gtgggcagca ctttgatatc gtaggctcgc gacagcgagc catcatctag cgccacgtcg
     7441 taaggaattg gccagctctg tttggcgcgc gcagcgtccg ccggattcga gtccacactc
     7501 actccgagga atgtaacgcc ggacttggag tgcttggcga acgcggcacc gacgctgggg
     7561 gccgtgcgtt cgcacactcc gcaccagctc gcgaacacct cgagcaatac cggccgattc
     7621 gcgtgctccg cgagcttgaa gcgcttctct tcgccgagca ggggtaagtc aatcggggct
     7681 gcggttttgc cctcggtggg tccggacatc ttgcgcgaga cgaaggtgac gagcgtgacg
     7741 ccgagcgcgc cgtaaagcgc gtaatccagc aggcgtgaga gccgcgcgtt cacggtgaca
     7801 acaagcgtcg tccgacgccc caacccagca gcgtgaaagc aatgacacct aggccgtggg
     7861 cgaagctcga gtgccaacct tcctgactcg cgcagccaac tccaatggcc aagctccccc
     7921 ccacaccgcc tacgagccca acgagcgcac cgctcaggct aggcgagagg ggatccgtgc
     7981 cgcgccacag caacaacaca ccacccgaca cgatcgcgct gaaaaccaag cccacgaggc
     8041 tgcagccgat ggcatgggca gctcgggcgc catgcgagaa ttcggcgaac gacacccatt
     8101 cgggtgccgc gtgcaccacg tacatcaaaa atccgaccgg cagcagcacc gccatgacca
     8161 gccgaattcg agtcgagact cgacgtcccg gagggcgtgc gaggcccgcc cacatcacgg
     8221 ccgcgagcac gatgccccag ccggccgcac ccaccagcgc gttgtgaaaa gtgccttcga
     8281 agcgcgctcg accgcgcgtc aaccacgcta gcgtgccgaa cacggaaagc gacaaaatca
     8341 ccgataaaac caagcgttgc cacgacttga gcccgcgctg cggcttcagc ttgtgcgtac
     8401 attgcccgca aatcgcggcc gaaacctcgc cactcggggc cttgggtgtg ggccaaggca
     8461 ggtccgccag aggatcgatg tcagcgcccg gtttcatgcg tttcggttga agcgttcgag
     8521 ttgtttgcgc agttgctcgt aaccgcgatg cacacgcagt ttgacggctg tctccgtggt
     8581 gttgagcacg gaggcggcgt ccgtcaggct caaccccgag atcttagtga gttgtatggc
     8641 ttcgcggtat gcctccggca agccgtcgag cgcgcgctcg agcgcttccg acaagtcggt
     8701 cgagaccgcg cgctcttccg agtgcggctc cggtaagttg ccgtccggac tcaggtcttc
     8761 agtgcgtgtt tgcgcgtgcc gtcgttcgtc caggaagctg cgtcgcgcta tggccaacaa
     8821 ccacggcaat acgggtgcac cccgcaaata gcttccccgc gcacgatgca ctttggagaa
     8881 agtgatctgc agcaagtctt cggcgcggtg ccgatcgcgc gtcaagcgca agaggtaccc
     8941 gaacaaggtg ggcgatacgc cgcgataaag ttcttcgaac gcgctcgggt ctccatcgac
     9001 gtagcgctcc attagaccgt cgaggcgcgc tcgtaagggg ttgccctcag ttctttcgcg
     9061 ttcttgagct tccccacggg gcgcctgctc ccgcgcgagc gtcggcaaca ccgctgcgtc
     9121 accgacgccg gccggtccca agctgggctg gctcgcgccg gcttggctcg cgccgcccat
     9181 accggcttgg ctcgcgcccg gtttggacgc ccgggatcgt cgcaggaagt ccacttccgc
     9241 gagctgtatc ccgattggag ggctaatgct accggccgtc acgaaccaca gaggcgagta
     9301 cgaccctccc ggcccggagg tttcaacccg gccggcatta atcctcacga actcaaatca
     9361 gggagcgcag gtcgagcatt tcctcgacgt ccggcatcag caccagctcg acgcgccggt
     9421 tttgctgccg gctctcatca ctgtcgttct tttggacggg gtccgtctcc ccataacctg
     9481 cggcgtgtaa ccgcattggg tccaagccgc cccccttcgc cttcccgtcg accggggcga
     9541 tcaggtacag gagcacgcgg cgcgcgcgca tcacactcaa gccccagttg tcgccaaacc
     9601 gcccaccgag cagaggcttg ttgtccgtgt ggcccgcgac ctggaagtag cgtcccgcga
     9661 gctggcggtc attgcgaatg acctcggcca ccgcgtccaa caccttctca ccctgttcgc
     9721 gcagcttgtc ttcgccagac gcgaacaaca cgtctccggg gaggcggatc accatgcggt
     9781 tgcgtcggat ctccaccttc agccccaagt ccgtgagctt cttcagcttg tctcgaagtt
     9841 gctcgaaccg ctgacgaatg cgctcgagct gcgcagcgcg ggctttgtat tcgtctagcg
     9901 cgcgcgccat ctcctctagg ttcttggaga gttccttgtt ctgagtacct gactgctcga
     9961 gctgctgatt gagggtctcc aggttcaccc ccatcttctg gagctgctcc ttcagcgcta
    10021 cgatttgacg cgctgccgtc tcgagctcgg cctgggtctg ctggcgcgct tgttgctcgt
    10081 gctggttctg gttcacgagc tcctggtact tgtgcagctg ggcttgccac tcgtcttcgg
    10141 aatgaccgca agcgaggagc agaaggccag ccaagccact caaccgcgac agctttcgca
    10201 tgtagttctc cggagttccg aacagcccat tgagcattcg gccccgccgt atgtcgaggg
    10261 cctttcggcg tcaatcgttt cgaaaccagg gatgtcgcac actggttagt ctcgggattc
    10321 gccgggggtc actcggtttc ggcttgcatt ctgctacttc gtcgtcactt gccggtcaca
    10381 cacgcgcgat accagcgcca gcatgggact catcttggta atcgaggacg agccggatat
    10441 tcggcaagtg ctcgactaca acttgcgggc agcgggtcac gaggtgattg aggcggagag
    10501 cgggacgcaa gggctcgatc gcgtgcgcac ccagcggcca gacctggtgc tcctcgacct
    10561 gatgttaccg gacctgccgg gcaccgaggt gtgtagagcc atcaaggaag atccaaccac
    10621 gcggggcatc tcggtggtca tgttgaccgc gcgcggagac gaaatcgatc gcgtggtggg
    10681 cttcgaagtg ggcgcggacg actacgtgac caagcctttc agcgtgcgcg agttgctcct
    10741 gcgcgtgcgc gcgattctgc gccgcaacga ggtcaaagtc gtcgagacca acacggtaga
    10801 gttcgggact ctgcgtgtcg accgcgacgc gcaccgcgtg tgggtgggcg acgaggaggt
    10861 cgagttgacc gcgctcgaat tcaagctgct ggtcacgctc tatgatcgca agaatcgcgt
    10921 tcagtcccgg gaagtcttgc tggataccgt gtggggcatc gagaacaaga tttcgacccg
    10981 caccgtagat gctcacgtga aacgcctgcg cgaaaagatg ggggcggccc gcgactacat
    11041 cgagaccgtg cgtggcgtcg ggtatcgttt cgccgaggcg cccgtgctgg atgcggactg
    11101 ctgggacacg cgctccgcct gacggctttc gtccagcgcg ccgcccacga gtttaccaac
    11161 gtgttccgcg cgtgcctgcg tgcagtcact cgcgagccgc aggctagcgc gatatcaccg
    11221 atgttggtgg ctggagacag gtgcacatgc agatgctcac gcacacggtc ctgctcgcgt
    11281 tgctagcgct gaccgccatc tcgacgctgc tcaccgccgt gtcgcacttc agcgtttcga
    11341 gagtattgcg ccgtcaagcc gcccctccta cgcggctccc agcgatctcc gtgctgaagc
    11401 cgctgaaggg ggccgatgaa gaactgtacg agaacttggt ctccttggct cggcaggatt
    11461 acccggattt cgagctgctg ttcggttgcg aggatcccaa cgaccccgct ttgccagtgg
    11521 tcg
//
LOCUS       DASTKU010000109         3197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_242652, whole genome shotgun sequence.
ACCESSION   DASTKU010000109 DASTKU010000000
VERSION     DASTKU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242652"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFQ61_03875"
     CDS             complement(<1..411)
                     /locus_tag="VFQ61_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953614.1"
                     /translation="MSWSKLSGHLHNAQGVLNARLHLKTAELRGPGVRLWGRPEIRNE
                     GTLIVGDRTRFISTVATLELAVGPGGMLDIGERCFINYGTSIAATRLVRIGAHCNIGT
                     HVLMMDNDFHRLEPERRNEMPESAPIILEDNVWLG"
     gene            544..1650
                     /locus_tag="VFQ61_03880"
     CDS             544..1650
                     /locus_tag="VFQ61_03880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9953615.1"
                     /translation="MSRYCFFTERQVGIGSAAAAVEPYLRENPEVTWVDVTYTREGGW
                     LERLPRNLTQRLGRATGTLRGVLQTREGLQQGPYDALFFLTHNPAVFRQLTLRRVPSV
                     LWTDVTPALLDQQAEQYAHPVDKSALARVVKDTLVQSTFRSAALCVGWSEWARRSFVT
                     DYGVPEQCTEVVPPGVDLLRFTARATTINSDALPRLLFVGGDFARKGGDLLLEVFRES
                     LQGKAELDIVTRDPVPNTPGVRVHHGLNAKSPELLALYRNAAAFVLPTRGDCYSIASL
                     EAMAMGLPVIVTDVGGISDIVERERSGFLIQPNDARDLRTALEAVLVSAERRRALGQR
                     GREIVEQRFDARKTADRLLELVRSAPERRARRGH"
     gene            1651..2709
                     /locus_tag="VFQ61_03885"
     CDS             1651..2709
                     /locus_tag="VFQ61_03885"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9953616.1"
                     /translation="MSRRARGKFHRFCAATIKGLAFAYPASLALVALALRFVGERHWV
                     TSLGLYLPRFPFGLPLLPLVGLLLLFGLRRLLWTQAVAAWLFFVPLMGWVAPLPTFGS
                     TDKSLSILSYNVNSGWGDYQKVADEIIARSVDLVFTQETFNDPTPFLDRLRARYPVVE
                     HHGDFVLASRYPIVSVTEPERIQMPGRLRSPRFIRYVVQTPVGPVSFYNIHPISPRGG
                     FYALRAGGFKNRLLSGQFLRGEGSESLQEMADLREKQIAAAAEMASHDPNPKVLLGDM
                     NLPGLSRVFGRYLSDYQDGFRKASWGFGYTFPNRDDFPAWMRLDRILASDEFKFLEFD
                     VGCQASDHRCVWTRLKKQ"
     gene            complement(2716..>3197)
                     /locus_tag="VFQ61_03890"
     CDS             complement(2716..>3197)
                     /locus_tag="VFQ61_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET9953617.1"
                     /translation="VATDTRIDSILAGCVLAVWRNPVLDAKSWEQRELSSVWLLVGFA
                     SVAASLVVRNAGFEQTFRYTLQSFGLLPFFIAAIRFHDRHVFRILNLRAVKYLGLLSY
                     SLYLMHTSVLWALEQKLHLGRFGQTLVALAILVALGTLIYRFIEKPCAGLRRRLSRV"
BASE COUNT          579 a         1027 c          990 g          601 t
ORIGIN      
        1 gccaagccat acgttgtctt ccagaatgat cggggcgctc tccggcatct cgttgcggcg
       61 ctcgggctcg aggcgatgga agtcattgtc catcatcaac acgtgcgtgc cgatgttgca
      121 gtgagcgcca atcctcacga gtcgagttgc cgcgatcgaa gtgccgtagt tgatgaagca
      181 acgctcacca atatcgagca ttccgcccgg gcccacggcg agctcgaggg tcgcgacggt
      241 ggagatgaag cgcgtgcgat cccccacaat caacgtgccc tcgtttctga tctcgggtcg
      301 gccccatagc cgtacccccg gaccacgcag ctccgctgtt ttcaggtgca aacgcgcgtt
      361 cagcacgcct tgcgcattgt gtagatgccc agagagtttc gaccagctca agcgctgatc
      421 gttttcacac ccgccttccc tgctgtcaac gttggcgcta cgctgcgcgc ttcagagcat
      481 gcgaaccacg atgatccgga ggttttgtgg acctctgtgg ctgcctctca agggggcgcg
      541 agcatgagca gatattgctt cttcaccgag cgccaagtcg ggatcggcag cgccgcggcg
      601 gcggtcgagc cctacttgcg cgaaaatccg gaagtaacct gggtagacgt gacgtacacg
      661 cgcgaaggtg ggtggctcga acggctgccg cgcaatctga cccagcgttt ggggcgggcc
      721 acgggaacac tgagaggtgt gctgcagacc cgagaggggc tgcagcaggg cccttacgac
      781 gcgctgttct tcctgaccca caaccccgcg gtctttcgcc agctgacgtt gcgacgagta
      841 ccgagcgtgc tctggaccga tgtcactccc gcgttgctgg atcaacaagc ggagcagtat
      901 gcgcaccccg tagacaagtc cgcgctcgcg cgcgtggtca aagatacact cgtgcagagc
      961 acgttccgca gcgcggccct gtgcgtgggc tggtccgagt gggcgcgccg ctccttcgtc
     1021 accgactacg gagtaccgga gcaatgcacg gaggtggtgc cgcccggggt cgacttgctg
     1081 cgcttcaccg cgcgcgcgac gaccatcaat tccgacgcct tacctcgcct gctgttcgtc
     1141 ggcggagact tcgcgcgcaa gggcggagac ctgctgctcg aggtcttccg agaatcgctg
     1201 caaggtaagg cggagctcga catcgtgacg cgcgacccgg tgccgaatac gcccggagtg
     1261 cgcgtgcacc acggcctgaa cgccaagagc cccgagctct tggcgctgta tcgcaacgcg
     1321 gccgcgttcg tgttacccac ccgcggggat tgttactcga ttgcgtcact cgaggccatg
     1381 gccatgggcc tgcccgtaat cgtgacggac gtcggcggca tttccgacat cgtagagcgc
     1441 gagcgcagcg ggttcttgat ccagcccaac gacgcacggg atctacgcac ggctttggag
     1501 gcggtgttgg tcagcgcgga acggcgccgt gcactcggcc agcgcggtcg tgagatcgtc
     1561 gaacagcgct tcgacgcccg caagaccgcc gatcgcttgc tcgagctggt gcgctcggcg
     1621 ccagaacggc gcgcgcgcag gggccactga atgtctcgcc gcgcgcgggg taagtttcat
     1681 cgattctgcg ccgctacgat caagggcttg gctttcgcct acccggctag cctcgcgctg
     1741 gtcgcgcttg cgttgcgctt cgtcggagag cggcactggg ttaccagtct aggcctatat
     1801 ttgcctcgct tcccattcgg cctccccctg cttccactgg tcgggttgct cctactattc
     1861 ggtttgcggc gtctgctctg gacccaagcc gtggccgcgt ggctgttctt cgtgccgctc
     1921 atgggttggg ttgccccgct gcccaccttt ggcagcacgg acaagtccct gtccatcctg
     1981 agctacaacg tcaactcggg ctggggcgac tatcagaagg tcgcggacga aatcatcgcg
     2041 cgatccgtgg atctcgtgtt cacgcaggag accttcaacg atccgactcc gttcctggat
     2101 cgactgcggg cgcgctatcc ggtcgtcgag caccacgggg atttcgtgct cgcctcgcgc
     2161 taccccatcg tatccgtgac agagccggag cggattcaaa tgccggggag gctgcgctcg
     2221 ccgcgcttca ttcgttacgt ggttcagacg ccggtcggcc ccgtctcctt ctacaatatt
     2281 caccccatct cgccccgcgg cggcttttac gccctacgcg cgggcggttt caagaatcgc
     2341 cttctctcgg gtcaattcct gcgcggcgaa gggtccgagt ctctccagga aatggccgat
     2401 cttcgggaaa aacagatcgc ggccgcagcc gaaatggcga gtcacgatcc aaacccgaag
     2461 gtgctgctcg gagacatgaa tttgcccgga ctgagccgcg tgttcggacg ctacttgtcc
     2521 gactaccagg atggctttcg taaggcgagc tggggcttcg gatacacctt cccgaaccgc
     2581 gacgactttc cggcctggat gcggctcgat cgaatcctcg cgagcgacga gttcaagttc
     2641 ttggagttcg acgtgggttg tcaggcctcg gaccaccgct gcgtttggac gcgtctgaaa
     2701 aaacagtgag ccggctcaaa cgcgcgacaa acggcgtcgc aaacccgcgc acggcttctc
     2761 gatgaaccgg tagatcaagg tcccgagcgc aaccaggatc gcgagcgcca ccagcgtttg
     2821 cccgaagcga cccagatgca gcttctgttc gagagcccac agcaccgacg tgtgcatcaa
     2881 gtacagcgag taagagagta accccaagta cttgacggcg cgcagattca agatccgaaa
     2941 cacgtgccga tcgtggaaac gaatcgcggc gatgaagaaa ggaagcagcc cgaagctctg
     3001 cagcgtatag cgaaacgttt gctcgaagcc ggcgttccgc acgaccaagc tcgccgcaac
     3061 cgaagcgaag cccacgagca gccacacgct cgacaactca cgctgctccc agctcttcgc
     3121 gtcgagcacg gggttgcgcc agacggcgag cacgcagccg gccaagatcg agtcgatgcg
     3181 cgtgtcggtc gcgacgt
//
LOCUS       DASTKU010000110         6384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_21919,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000110 DASTKU010000000
VERSION     DASTKU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21919"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="VFQ61_03895"
     CDS             <1..1519
                     /locus_tag="VFQ61_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953618.1"
                     /translation="GSSLEFLKVGRKLELEDLEHGARRGAEIDGISVIVDPSTQVPQL
                     IVALKFEGQESTPEPSVADVKGGSSRAEGLRIPAEAVTTSSRAKAPAGQESASKLRIK
                     DEPASAESAKSATSKSLEADRDAADDSDDDQPEMRGKVAVLASQAEQKAKAAGEVLAR
                     LSHGAAEGAAGWFKGAQERVLKLRSAKAPERRTTAPPKSMRASEKPKLRQQSGSKDDA
                     DVDAAPVANKSRRTLAVAACVGVVGITALVFATRGSTPNAPRASDPVAAANGLNADGA
                     PANGSAVALAPGAPRPLRDPGPPAVVTANVPLFGPTPLATTEPAPLGPVPGSDAAFIE
                     DAEKAAAKASLRGGSNLDESFESADETSERAIERNPSKDGKLARDAATKKPEDVSPWG
                     HGKMHEPIVHRLRLDGPGAAINGAVEPTGFTVTIPDRKVMEPTTSITKREPRLARLRA
                     ANTGSGAQISFRFKDGVPSYRVRLRRDYVEFLISSPEEKSTKGEGEGSRKKSDKR"
     gene            1541..2989
                     /locus_tag="VFQ61_03900"
     CDS             1541..2989
                     /locus_tag="VFQ61_03900"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9953619.1"
                     /translation="MVDRPYIFFELTNSLCSQCLRKLEAKILIEQGSVWMLKRCPAHG
                     PERVLLSTDVEYYQLQRRFLKPGQLPRHFGTAWERGCPYDCGLCPDHEQHSCLSLVEI
                     TDHCNLECPVCYAGSSPTRREYRTLGEVEAMLDAVVRNEGQPDVVQISGGEPTLHPQL
                     FEILRAAKQRPIRHLMLNTNGLRLANDPELVSQLAAFAPGFEIYLQFDSLRPEPYLAL
                     RGADLLEKKLRALTELERVGLSTTLVVTLKKGLNDRELGELVRFALGFRCVRGITFQP
                     VQDAGRNLAYDADTQRLTLSEVRSGLLAQQALFGERDLIPVPCHPDALCMGYALKANG
                     GATALSGLLDPETLLTSAGSTIVYERSAELKEHLLKLFSTAASPASSENNLKQLLCCL
                     PSLEAGHGAADLTYENVFRVLIVQFMDAFSFDVRSVKRSCIHMAQPDGRMIPFETYNL
                     LYRDPRRLAQIIEGGAVETGLTPLRVRRAELS"
     gene            3010..3879
                     /locus_tag="VFQ61_03905"
     CDS             3010..3879
                     /locus_tag="VFQ61_03905"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HET9953620.1"
                     /translation="MHGWLYPLLSCVGLLISAALGQKLLRAAEGAPEATRPSMPASVH
                     PHLVFAGALFGALIGAKLGYFFAEWLPQRLSQSPLASFEALLFGRTVLGALLGGYAGV
                     ELVKRLIHYRRATGDGFAVMAPLSIGLGRIGCWGEGCCLGVEHARSWFTLDDAQGIAR
                     WPAVPVELAFNVLFGAAVAYYVLRMKPLPFLKGQLFHVYLVSYGAFRLWHETLRETPH
                     AHGMSGYQGLAVLVVLLGGVRFVQRQRGKTKWEDEGAAGDGEEPVEQDPPEHDAQQGQ
                     REVRAEAPREEDP"
     gene            complement(4079..5158)
                     /gene="tsaD"
                     /locus_tag="VFQ61_03910"
     CDS             complement(4079..5158)
                     /gene="tsaD"
                     /locus_tag="VFQ61_03910"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET9953621.1"
                     /translation="MRVLGIETSCDETCAAVVDETGQVLSDVVHSQVELHRPYGGVVP
                     ELASRDHLRNVRPVVEAALARAGMQPTEIDGIAVTCRPGLTGALLVGVQVAQGLAFAL
                     ARPLVGVDHLFGHLLAAFLRFPGGTAEPPPEFPFVALLVSGGHTALYRVDGPTLDAVQ
                     ELGATRDDAAGEAFDKVAKLIGLGYPGGPIIDRLAKLGNPDGIELSLPMPSANSPEFS
                     FSGLKTNVARYVEKHGLPQEGPALYDLCATFQRRVVETLVKKSVAAARREGVQTLVLG
                     GGVAANRELRESAARACSKHGMRLFVPAFSACTDNGAMIAYAGALRLARGEDDTGTIS
                     TTPVTALAQVTRKGRGRRGVRLPGA"
     gene            complement(5209..5817)
                     /locus_tag="VFQ61_03915"
     CDS             complement(5209..5817)
                     /locus_tag="VFQ61_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953622.1"
                     /translation="MPLLPRLRLRGKAVAIWALVLLALTGAPGCSKLSRFDTGDGKEP
                     FCGKLVQTPPFSAGLMPDNVQPRTFELKLNLDMKALTSRGELASIVGSISSSDGAFGL
                     CHTETSRALFEDAPLRTIPELDHDQLSLLEFGEGREYSFFAWADSTCQGTILTLVSLM
                     RNDEVEIRLFKPAALPTPNARPQEQPGFGLFRLTRREFACSF"
     gene            complement(5872..>6384)
                     /locus_tag="VFQ61_03920"
     CDS             complement(5872..>6384)
                     /locus_tag="VFQ61_03920"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9953623.1"
                     /translation="ATALAARIAEQHVAAFVGWTEHPAPEVRAFAVAFLAQQAADIAV
                     KATVAAVKDADVNVRNVALASLTPRHAAAAPELIRLSESEDWSVRLAAVRAQGAILEA
                     TGPAAVIASLSQRATADRTAIVREAALAALAKGAKPAARPVLERARDSDPEPHVRAAA
                     GAWLSSFSAP"
BASE COUNT         1183 a         2013 c         2149 g         1039 t
ORIGIN      
        1 gggttccagc ctggagttct tgaaggtcgg gcgcaagctg gagctcgaag atctcgagca
       61 cggcgcgcga cggggcgcgg agatcgacgg gatcagcgtg atcgtggatc cgagcacgca
      121 agtgccgcag ctgatcgtgg ccctgaagtt cgaagggcaa gagagcacgc ccgagccgag
      181 cgtcgcggac gtgaagggtg gctcgagccg cgcggagggg cttcgtatcc cggccgaggc
      241 ggtcaccacg agcagtcgtg cgaaagcccc cgcgggccag gagtctgcga gcaagctgcg
      301 gatcaaggac gagcccgcct ccgcagagtc cgccaagagc gcgacctcca agtcgctaga
      361 ggccgatcga gacgcggcag acgacagcga cgacgaccag cccgagatgc gcggcaaggt
      421 cgcggtgctg gcctcccaag cagagcagaa ggccaaagcc gcaggtgaag tgctcgctcg
      481 attgagccac ggtgcagcgg aaggcgccgc ggggtggttc aaaggggcgc aggaacgggt
      541 gctgaagctg cgcagtgcca aggcaccgga acggcgtacg acggcgccgc cgaagtcgat
      601 gcgcgcgtcg gaaaagccaa agctccgtca acaaagcggc agcaaggacg atgccgacgt
      661 cgatgccgca ccggtcgcca ataaatcgcg gcgaacgtta gcggtggccg cgtgtgtcgg
      721 agtggtcggg atcacggcgc tggtgttcgc gacccgtggt tccacgccga acgcgccgcg
      781 cgccagcgat ccagtggcgg ccgccaatgg cttgaacgcc gacggcgcac ccgcaaacgg
      841 ttccgcggtg gcgctcgcgc cgggtgctcc ccggccgctg cgcgatccgg ggccccccgc
      901 ggtcgtgact gccaacgtgc cgttgttcgg acccacgccg ctcgcgacga cggagccggc
      961 cccgcttggg ccggtgcccg gcagcgacgc cgcgttcatc gaagacgccg aaaaagcggc
     1021 cgcaaaagcc agcttacgcg gcggttcgaa cctggacgag tcattcgaat cggcggacga
     1081 gacgtcggag cgagccatcg aacgcaaccc ctccaaggac ggcaagctcg ctcgggacgc
     1141 ggcgacgaag aagcccgaag acgtgagccc ctggggtcac ggcaagatgc acgagccgat
     1201 cgtgcaccgc ctgcgtctgg atggccctgg cgcggccatc aacggcgccg tcgaaccgac
     1261 cggcttcacg gtgacgatcc ccgaccgcaa ggtgatggag ccgaccacga gcatcaccaa
     1321 gcgcgagcct cgcctcgccc gtctgcgcgc agccaacacg ggcagcggag cgcagatttc
     1381 gttccgcttc aaggacggcg tgccgagcta tcgcgtgcgg ctgcgtcgag actacgtcga
     1441 gttcttgatt agctcgcccg aggagaaatc caccaagggt gagggcgaag gctcgcgtaa
     1501 gaagtcggac aagcgctgaa cccaccacgc ccgagccgcc gtggtcgatc gcccctacat
     1561 cttcttcgag ctcacgaata gcctgtgctc gcaatgccta cgtaagctcg aagccaagat
     1621 cctgatcgag cagggcagcg tctggatgct caaacgctgc cccgcccacg gccccgaacg
     1681 ggtgctgctg tcgacggatg tcgagtatta ccagcttcag cgtcggtttc tgaagccggg
     1741 gcagttgcca cggcacttcg gaacggcctg ggagcgcggt tgcccgtacg attgtgggct
     1801 gtgtccggat cacgaacagc acagctgcct gtcgctcgtg gagatcacgg atcactgcaa
     1861 cttggagtgc ccggtttgtt acgcgggctc ttcgcccact cgacgtgagt accgaacgct
     1921 gggcgaagtg gaggcgatgc tcgacgcggt ggtgcggaac gagggtcagc ccgacgtggt
     1981 gcaaatctcg ggcggcgagc cgacgttgca tccgcagctg ttcgagattc tgcgcgccgc
     2041 gaagcagcgc ccgattcgcc acttgatgct gaataccaac gggctgcgtc tcgcgaacga
     2101 tccggagctg gtcagccaac ttgcggcttt cgcgccgggc ttcgagatct acttgcagtt
     2161 cgactcgttg aggcccgaac cttacctggc cctgcgcggc gctgatttat tggaaaagaa
     2221 gctgcgcgcg ctgaccgagc tcgagcgcgt cggcctctcc accacattgg tagtcacgct
     2281 caagaagggc ttgaacgatc gagagctcgg cgagctggtg cgcttcgcgc tgggctttcg
     2341 ttgtgtgaga gggatcacgt tccagccggt tcaagatgca gggcggaacc tggcttacga
     2401 tgccgacacg cagcgcctca cgttatccga ggttcgcagc ggcttactcg ctcagcaagc
     2461 tctgttcggg gaacgagacc tcatcccggt gccgtgtcac ccggatgcgc tctgcatggg
     2521 ctacgcgttg aaggcgaacg gcggcgcaac ggcactttcg gggctgctcg acccggagac
     2581 gctgctgacg agtgccggta gcacaatagt gtacgagaga tcggcagagt tgaaagaaca
     2641 tctgctgaag ctcttctcga cggcggcgag cccagcctcg agcgagaaca atctcaagca
     2701 actgctgtgt tgtttgccaa gcttggaggc gggccatggt gcggcggatc tgacctacga
     2761 gaacgtgttc cgggttttga tcgtgcagtt catggatgcg ttctcgttcg acgtgcgcag
     2821 cgtgaagcgc tcctgcatcc atatggcgca gcccgacggg cgcatgattc cgttcgagac
     2881 ctacaatctg ctgtatcgag acccgcgccg ccttgcccaa atcatcgagg gcggggcggt
     2941 cgaaacggga ctcacgccgc ttcgagtccg acgtgccgag ctgagctgag ctgagacacg
     3001 agcgggacga tgcacggctg gctctatccg ttgctgtcct gcgtggggtt gctcatctcg
     3061 gcggcgctcg ggcagaagct gctgcgcgct gcggaggggg cgccggaagc aacgaggcct
     3121 tcgatgccag cctcggtgca cccgcatttg gtgtttgcgg gggcgttgtt cggggcgctg
     3181 ataggcgcca aattgggcta ctttttcgcg gagtggttac cgcagcggct ctcgcagagt
     3241 ccgcttgcgt ccttcgaggc gttgctattc gggcgcacgg tgctgggcgc gctgctcggt
     3301 ggctacgcgg gagtggagct cgtgaagcga ctgattcact accgtcgcgc gacgggagat
     3361 ggcttcgcgg tgatggcgcc actctcgatt ggtttaggtc gcataggctg ctggggagag
     3421 gggtgttgtt tgggggtgga gcacgcgcgg agttggttca cgctcgacga tgcgcagggc
     3481 atcgcgcgct ggcctgcggt acccgtggag ctcgcgttca acgtgctgtt tggggcggcg
     3541 gtggcgtact atgtgctgcg gatgaagccg ttgcccttcc tgaaggggca gctgtttcat
     3601 gtctatctag tgtcttacgg cgcgttcagg ctttggcacg aaaccttacg cgagacgccg
     3661 cacgcgcacg gcatgagcgg gtaccaaggg ctggccgtgt tggtcgtgct cttgggaggg
     3721 gttcgtttcg tgcagcggca gcgaggcaag acgaaatggg aagacgaggg cgcagccggc
     3781 gacggcgagg aacccgttga gcaggatccc cccgagcacg acgcccaaca gggtcaacgc
     3841 gaggttcgcg cggaagcgcc gcgcgaagag gatccctaac cacaccaagt aaaacggaat
     3901 caggagcagc aacgcgctcg cgaacacggc gacccaatcc gaagaagtca gccgtgcgag
     3961 caatggaatg ctcaagagca gaacactggg tagcaccagc aacccggccg ctgccaataa
     4021 cgagagcggt ggtttgggcg gaggttgatc cgagggttct tccgtcatgc ccactccttc
     4081 aagcgcccgg caaccgcacc ccacggcgac cgcgcccttt gcgggtcacc tgagccagcg
     4141 cggttacggg cgtcgtgctg atcgtacccg tatcgtcttc acctcgggcg agccgcaacg
     4201 ctcccgcata agcgatcatc gcaccattgt ccgtgcaggc cgagaacgcg ggcacgaaca
     4261 gccgcatccc atgtttggag caggcccggg ccgcgctctc gcgcagctca cgattggcgg
     4321 ccactcctcc gcccagaacc aaagtctgaa caccctcgcg gcgcgcggcc gcgacggact
     4381 ttttgacgag tgtctcgacc acgcgccgct gaaatgtggc gcacaaatcg tagagcgcgg
     4441 gcccttcttg aggcagcccg tgtttctcca cgtagcgagc cacattggtt tttagccccg
     4501 agaagctgaa ttcaggggaa ttggccgagg gcatcggcaa agagagctcg atgccgtcgg
     4561 ggtttccgag cttcgccaaa cgatcgatga tcggtccacc ggggtagccg aggccaatca
     4621 gcttggccac cttgtcgaag gcttcacccg cggcgtcgtc gcgcgtggcg ccgagctctt
     4681 gcaccgcgtc gagggtcgga ccatccaccc gatagagagc ggtatgtccg cctgaaacca
     4741 gcaacgcgac gaacgggaac tccggcgggg gctccgccgt accgcccggg aagcgcaaga
     4801 atgcggcgag caaatgtcca aagaggtggt cgacgcccac cagagggcgc gccagcgcga
     4861 acgccaagcc ctgcgccact tgcaccccga ccaacaaggc gcccgtgagg cccgggcgac
     4921 aagtgaccgc aatgccatcg atctcggtcg gctgcatccc ggcacgcgcg agggcggctt
     4981 cgaccacggg ccgcacattg cggaggtggt cgcgtgaagc aagctcgggc actaccccgc
     5041 cgtaggggcg gtgcagctcc acttggctgt gcacgacgtc gctcagcacc tgaccggtct
     5101 cgtcgacgac ggccgcgcag gtttcgtcac acgacgtttc aatcccgaga acacgcatca
     5161 cttggcatcc tgatcctgcg gtcgactcag ctcaccccat agcagtgcct agaacgaaca
     5221 cgcgaactca cgccgggtca gccgaaaaag gccgaaacct ggctgctctt gaggccgagc
     5281 attgggcgtg ggtaacgccg ccggtttgaa caaacggatc tcgacttcat cgtttcgcat
     5341 cagcgacacc agtgtcagga tcgtgccttg gcacgtggag tccgcccacg cgaagaagct
     5401 gtattcgcgg ccttcgccga actcgagcag cgaaagctgg tcatgatcga gctcggggat
     5461 agtgcgtagg ggagcatctt cgaacaacgc gcgcgacgtc tccgtatgac acaagccgaa
     5521 cgcgccgtcg ctgctcgaga tgctgccgac gatgctcgcg agttcacccc gggaggtgag
     5581 cgccttcatg tcgaggttca gcttcagctc gaacgtgcgc ggctgcacgt tgtccggcat
     5641 gagccccgcc gagaacggtg gtgtctgcac gagcttcccg cagaacggct ccttaccgtc
     5701 accggtatcg aaacgcgaaa gcttgctgca gcccggagcg cccgtcagcg cgagcaggac
     5761 tagcgcccag atcgcgaccg ccttgcccct gaggcgcaat cgcggcagca atggcattcg
     5821 ggccgagcga ggttcagtca ccaggctcgg gagcattgca cagcggatcg gctatggcgc
     5881 agaaaaagag ctcaaccaag cgccggccgc ggctcgcacg tgaggctctg ggtcgctgtc
     5941 gcgggcacgc tcgagcacgg ggcgagccgc gggtttggct cctttcgcca aagcggcgag
     6001 cgccgcttcg cggacgatgg ccgtccggtc cgcggtcgcg cgctggctca gagaagcaat
     6061 gacggctgcc ggcccggttg cttccaggat cgcgccctgc gcacgcacgg ccgccaagcg
     6121 cacgctccaa tcctcgctct cgctcaagcg aatcagctcg ggcgcggcgg cggcgtgacg
     6181 cggggtgagt gaggctaagg cgacgttgcg aacattcaca tccgcgtcct tcacggccgc
     6241 gaccgtagcc ttcaccgcga tgtccgcggc ttgctgcgcc aagaacgcca ccgcgaaggc
     6301 ccgcacctcg ggcgccggat gctccgtcca gccgacgaaa gcagctacat gctgctccgc
     6361 gatccgggcg gccaacgcag tggc
//
LOCUS       DASTKU010000111        14192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1582446, whole genome shotgun sequence.
ACCESSION   DASTKU010000111 DASTKU010000000
VERSION     DASTKU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1582446"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1535
                     /locus_tag="VFQ61_03925"
     CDS             90..1535
                     /locus_tag="VFQ61_03925"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9953624.1"
                     /translation="MMSPIRNDAAQAAPSTVPATSEIQRRAQRCMGSVLVVEDDGIAA
                     IDIEQTLESNGYDVAGVATSAEEALALARTLRPDLVLMDIHLRGAMDGIETAQQLQRE
                     LALPVVYLTANADEVCLRRATETEPYGYILKPFESIDLRICVDVALRRSRTDRLRRDS
                     DMRYRRIVQQTSEGVFSTNADQRITVVNAQFAAMLGYTSTELVGRSPFDFMDERDHQL
                     GARSFARCRQERACRFEARLLRVDGAEFWASISLSSAADPAGEGMIGVVQDVSDKKAS
                     EEALRAALAHLKRENGALADLAIRDDLTGLYNRREFERRLDSCLEEQPGRDAALSVVL
                     ADIDHFKAINDTYGHHAGDEVLRELGGILGVSLRQTDLACRYGGEEFALLLPNTPGAS
                     AVALAERIRKNVAAHAFWVLDLTGERRNISVTLSLGCAVRQSSRCSGEDLLREADQAL
                     YSAKTAGRNRVVGIDPLPELAATSSGFPNPR"
     gene            complement(1512..2303)
                     /locus_tag="VFQ61_03930"
     CDS             complement(1512..2303)
                     /locus_tag="VFQ61_03930"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953625.1"
                     /translation="MTSPLRCLSLRTEAPHGVSSQSKDPVYLLLVSLRDAADTTRQAL
                     SFDRESQLEWLGEAALSGASLAILSTPNAVELYSTQRSRIEAFRPALRAFRARTKHQP
                     EVGQLRTVELCGKAAALHLLRRAAGLKGRLGDLQIAAEIHAAAALSANSAALGPALSS
                     LFLAAGNVSRRVLQETELGDVTVANSVRELDRFAAERIAAEELCAWEAQRNEAQRSMQ
                     RRSLVPRESISRSVPASAPPSEYSEEPGSLIRVRAAARLPGVREA"
     gene            complement(2522..2860)
                     /locus_tag="VFQ61_03935"
     CDS             complement(2522..2860)
                     /locus_tag="VFQ61_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953626.1"
                     /translation="MSENTEAQSTDERPATPVFNVVGGGVARIVRADADNVMLETGAP
                     SPPGSTLRLLVLNREVGVKVRGCRRIEGSMQGRLEGAMDSAGSALFRIEGRWVSLSRE
                     QREVVTTKSV"
     gene            complement(2885..3367)
                     /gene="smpB"
                     /locus_tag="VFQ61_03940"
     CDS             complement(2885..3367)
                     /gene="smpB"
                     /locus_tag="VFQ61_03940"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET9953627.1"
                     /translation="MARGKTTTLGDRLVSKNRRATFDYEITDTFEAGLVLIGSEARAL
                     REGAADLSDAWIDIDNRGEAWVKGMRIPPLKHAAFGHNDTRPRKLLLHRAQIERLRGL
                     TERDGMTLISTKCYIKNNHAKLEVAVARGKKQYDKRQSIKTKEADREAVAAIRRGRRD
                     "
     gene            3494..8797
                     /gene="uvrA"
                     /locus_tag="VFQ61_03945"
     CDS             3494..8797
                     /gene="uvrA"
                     /locus_tag="VFQ61_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9953628.1"
                     /translation="MLHTRLESARTHNLKDVSLELEPGTLVVLSGVSGAGKSSLAIDT
                     LYAEGQRRFVESFSPYARQFLERLERPPMQSLEPVPAAVAVDRRAPVKSSRSTLATLA
                     DLEPYFSALFLREAVPVCPEHQVEGTRLDPERAAARVGAELGVGRALVTYPLGVDRVE
                     SYLGVRDNLAQQGYRRLWVSGELLDIERAKPSQVLAAGSEVGVVVDRVTTTERTRVAQ
                     AVEQAWQRGAGVCVVRSERESFVLRQGLGCGVCGRELPEPSAGLFSYESPVGACATCR
                     GFGRTLGIDLDKVIPDKTLSLKAGAIRAFRGKSTSYERGELAKLCRRHGIPFDRPYAE
                     LGAAQQRLVLEGDGSFEDGLFPGVLGWFRWLETRTYKMHVRVFLSRYRAYDLCHGCGG
                     RRLNATALAFRVDGLDLAQWHALEVGAALEKLAQLETRSAQGELARKELVSRLDYLKR
                     VGLGYLTLDRQARTLSGGEAQRVTLTAALGTSLNNALFVLDEPTIGLHPSDIEPLITL
                     LHELAARNNCVLVVEHDPAVMAAAGRVIELGPGAGSEGGRIVGDGVPSALGRGTGVTA
                     QTLIGLPPRQRPPRAWAHELRVVGATEHNLKLPEVRIPLGVICAVTGPSGSGKSSLVV
                     DTVYRALALRAGELDVEAPGHHERLEGAERVTRVTLVDQTPLGRTSRGNAATYTKAWD
                     CIRTLYAREPSAARLGLQAKDFSFNVEGGRCEACAGEGYETVEMQFLSDVRLICPTCQ
                     GRRFKPHVLSVERHGVSIADVLNMTVTDALHAFRAEASVQRALGPLARLGLGYLRLGQ
                     PLSTLSGGEAQRLKLCRALAELRPGELLVLDEPSAGLHVSEVEKLLGVLDTWVDSGGS
                     VLMVEHDLDVIANADYVIDLGPGGGRRGGELVACGSVDELIERGSRTGEALARRLKRT
                     GRTHGGARASSRKPTPSVRSEAVLRVEQAREHNLKNVSIGLPHGAFSVVTGPSGSGKS
                     TLAFDVVFAEGQRRFLETLTPYARQFLPSMPRPDVDRVSGVPPAIALPQRTARAGGLS
                     TVATVTEVAHYLRLLYAKLGTPHCPEHQTPIARRSFDELRADVHAQRKKSYLLAPVVQ
                     QRKGTYLDVFSSAARAGILQAFVDGALVSTQEPPKLARSREHTIDLLIASPASPKTFE
                     PATLRRALDFGRGSLKLRTELGDELLFSSEAACPICGFSPPELDPRWFSFATKQGRCE
                     ACEGNGVLETERTVGRGKTRRVEVVRQVCAACRGQRLNPIVRSVRLDTESYPEFTARS
                     VRAALERARRLRFDARLQPVAAPLIGELTRRLQFLCDVGLDYLALDRHAATLSGGEMQ
                     RLRLAAQLGAGLTGALYVLDEPTIGLHPRDTDRLIRNLKQLVELGSTVLVVEHDLEIM
                     RAADYLIDMGPSGGAGGGRVVAAGSPSQVLALPDSATARVLANPPYIRKALPVKSTDA
                     RLLLSGATQHNLKGVDLNLVLGRFNVVCGVSGSGKSTLVSHVLLPALRRALGLVAEEP
                     GAHAGLTGFDALQRALSVDQSPIGRTPRSVPATFLGIWDEIRRLFAATPEAQVAGFNA
                     TRFSFNSKQGGACTTCDGQGSITHEMSFLPDVVAPCPTCEGLRFEPRTLAVRYLGLNV
                     GEVLKLTAEQACERFEHHPRLKAPLRTLTELGAGYIQLGQGSHTLSGGEAQRLKLALE
                     LTAGARHERTLYVLDEPTTGLHPSDVERLLSVLGRLVERGDTLVVIEHHPQVIASADW
                     IVELGPEGGENGGKIVAEGTAREVARRKSATASVIQRILSL"
     gene            9025..9780
                     /locus_tag="VFQ61_03950"
     CDS             9025..9780
                     /locus_tag="VFQ61_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953629.1"
                     /translation="MKSTRTLFIGLTAVICSLAVACGSDDDDAGPATGGNSAGSSAKG
                     GNSTSGGTSSTAAPSGGRSSGGTTSSGNGGTSVGRGGATQNAGGASEAGSDTGGAGPE
                     AGAPSEAGAGPTGGRVGMGGRSGSGGRTGTATGGRTSSGGASSGGRTSSGGMTPGGAA
                     GEAGAGGASADACPETRPRRGDLDCSDPGLVCTYGQVSCTCERARGGAQFSCSTTETT
                     TGGRSGTGGASSGGTSNTGGRRGRPTPAAGAEG"
     gene            10062..12965
                     /locus_tag="VFQ61_03955"
     CDS             10062..12965
                     /locus_tag="VFQ61_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HET9953630.1"
                     /translation="MRKSVWLRSAVLSLFVASACSSEKDPPLAENAGKGGAASNAGGT
                     RASGGAPNAGRTNTGGIGDARGGDTYGGTYYGGAASGGTNEAGNGNAGEAGENAGVAG
                     SGGEAGASAALRDPSHGSPIALAPDERLAVVVNRDVGSVSIVELSAPEAGLVPEVRLK
                     RELSLGAGSEPWQVVISPDSDTAFVVLKRDQRLVRIRHLHGEPKLDGTVSVGSEPTSV
                     ALSPSGAFVFVANFVDGTLSVLNAETLRLVRTVDLNRALVDGGYLGQVEARPALAHPR
                     SILVTNNRDDQDGDEAVYITEYFAQQLEPEAADGSNADTRKAGLVYRVSLSDYSVQTI
                     ELAALADMGFRAGDGKAAGCYPNQLQSIAAAGKFAYVLSVCASPRGPLGVRVTTTACK
                     TVADCSGLGLVEPTCALPFGGAPSALCTDVASVKTTTAPVVSVIDLEQGREVSGSARN
                     LNAEFESLFDARGTPATDRRLPLFASDMSFVPDTSVAYVSANGTDAVFRLVFDAAKGT
                     LTSVGASTQSFIDLFPSGIAKERAGKNPIGVVSGGNTRKYALALNDVGRNLSVIDLNA
                     QALAGGVANARVIGVADQPERGSDAERILRGKRFFDTGVGRWSLNGEGFGACQVCHSD
                     GLTDNVTWYFARGPRQATSLDGSFASNDPTDQRIFNWTAIFEDVPDFEANTRDISGGV
                     GAIVSKVSAPPQNSDRIDFVGLKHAGLNGSSAQAADPKNPLGFAEPPLLEDWADIERY
                     IQTIRSPRAPSTLIPSQVAEGEKLFVSAGCQGCHGGAKWTVSRRFYTSSTETNARLGT
                     TPFEIPAGFPQELLPARQKANQLLRFGGSNPAALDQILCAIRPVGTFDVAEAGVGIAE
                     LRADMKTVAQGDGDPAGEGRGYNPPSLLGMTLGAPYLHAGSARTLEALFAPPFEQHYR
                     ALSPNFLAESDPAVRQRQIDALVAYLSAVDEGTPIVDLPKPGPLGGSLCPESF"
     gene            complement(12988..>14192)
                     /gene="rlmN"
                     /locus_tag="VFQ61_03960"
     CDS             complement(12988..>14192)
                     /gene="rlmN"
                     /locus_tag="VFQ61_03960"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET9953631.1"
                     /translation="ARARGGGPQCRLSPALGPRLRVNAACRVMTPRASVPPVTRPLPG
                     LLLSELASELGSRTRAIQTLKWLYGLSSLPERIPSSIPNVARDVFRPFAERHGLLVPK
                     LLSEHRSQDGTRKFVFDLAGSSVESVLIQESQRSTVCLSSQAGCTRRCAFCATQSLGF
                     ERMLRAEEMLAQYWACRMQAPPDRPAKNVVFMGMGEPMDNLEEVLRAVELLNQGPAPG
                     LRLSSVTVSTSGVVPGMRRFLRECKASLALSLNATTDEQRERLMPHNRTWPIAALLEV
                     LREDAAATCKTRDTLVEYVLLANVNDTDADADRLISLLRDLPVRINLIPYNAHAQSAF
                     VAPSPERVRAFQRRLLDAGYRCFARRSHGPDIAAACGQLALVALDPKRSLKVPAVEPV
                     SLRASALG"
BASE COUNT         2386 a         4457 c         4823 g         2526 t
ORIGIN      
        1 aggcacgttt gcttcggcag gcgcgccacg ggcgctcggg tgcgatcccc gaaacaagcg
       61 ctgcaacaga aggaagggtg aacgcgctca tgatgagtcc catcaggaac gacgcggcac
      121 aggctgcacc atcgacggtc ccagcgactt cggagatcca acggcgcgcg caacgctgca
      181 tggggagtgt gctggtggtc gaggacgatg ggatcgcggc catagacatc gagcagacgc
      241 tcgagtccaa cggctatgac gtcgcgggtg tcgccacgag cgccgaagaa gcgctcgcgc
      301 tcgcgcgtac gctgcgaccc gacctcgtct tgatggacat tcacctgcgg ggggccatgg
      361 acggcatcga gaccgcgcaa cagctgcagc gtgagctcgc gttaccggtg gtctacttga
      421 ccgcgaacgc ggacgaggtc tgcttgcgta gggccacgga aaccgagccc tacgggtaca
      481 tcctgaagcc attcgagtcg atcgatttga ggatttgcgt cgacgtcgcg ctgcgccgct
      541 cgcgtaccga ccggctccgg cgtgatagcg acatgcgtta ccggcgcatc gttcaacaga
      601 ctagtgaagg cgtgttctcc accaacgccg atcagcgcat taccgtggtg aacgcgcagt
      661 ttgcggccat gctcggctat acgagcacgg agctcgtcgg gaggtcaccg ttcgatttca
      721 tggacgagcg agaccatcag ctcggcgcgc ggagcttcgc gcgttgccgg caagagcgag
      781 cttgccgatt cgaagcgcgt ttgttgcgcg tggacggagc cgaattctgg gcttcgatct
      841 cgttgagctc cgccgccgat cccgccggtg agggcatgat cggcgtggtg caagacgtat
      901 ccgacaagaa agcttcggag gaggcgctgc gggccgccct cgcccacctg aaacgcgaga
      961 acggagcgct cgctgacttg gcgattcgtg acgacctgac cggcttgtac aaccggcgcg
     1021 agttcgaacg gcgtctggac agctgcctgg aagagcagcc cgggcgcgat gcggcgctga
     1081 gcgtcgtgct cgccgacatc gatcacttca aggccatcaa cgacacttac gggcaccacg
     1141 ccggggacga ggtgctgcgt gagctcggcg gaatcttggg tgtgtcgcta cgccaaaccg
     1201 atctggcctg tcgttacggt ggggaggagt ttgcgctcct gctaccgaat acacccgggg
     1261 cgagcgccgt ggctttggcc gagcgcatcc gcaagaacgt ggccgcgcat gcgttctggg
     1321 tgttggactt gacgggcgag cggcgcaaca tctcggtcac gttgagcctc ggctgcgcgg
     1381 tgcgccagag ctcgcgctgc agcggcgaag atttgctgcg agaggccgat caagccctgt
     1441 attccgcaaa aactgcgggt cgcaaccgcg tagtcgggat cgatcctttg cccgagttgg
     1501 cggcgacgag ctcaggcttc ccgaaccccc ggtaagcgag ccgccgcacg cacgcggatt
     1561 aggctgcccg gctcctcact gtactcggaa ggcggtgcgc tcgcgggtac gctcctggag
     1621 atgctttcgc gcggcaccaa gctgcggcgt tgcatggaac gttgggcctc gttgcgctgg
     1681 gcttcccagg cgcagagctc ttccgctgca atgcgctcag cggcgaagcg gtcgagttcg
     1741 cgcaccgagt tggcgaccgt cacgtccccg agctcggtct cttgaagcac gcgccggctg
     1801 acgttgccgg cggccagaaa caaggaactg agcgcgggcc ctagcgcggc cgagtttgcg
     1861 gagagcgccg ccgctgcgtg gatttcggcg gcaatttgca ggtctcccaa gcgtcccttg
     1921 agaccggccg cgcgtcgcaa caaatgcagg gctgccgcct tgccgcacaa ctcgaccgtg
     1981 cgcagctgac cgacctcggg ttgatgcttc gtccgggcgc ggaaggcgcg caaggcaggg
     2041 cggaacgctt cgattcgcga ccgctgcgtg gagtagagct caacggcgtt gggcgtcgac
     2101 aggatcgcca gcgaagcacc ggacaatgcg gcttcaccga gccactccag ctggctttct
     2161 cgatcgaagc tcaaagcctg ccgagtggtg tccgcggcat cgcgtaagct gaccagcagc
     2221 aggtagacag ggtccttgct ctgggacgaa acaccgtgag gggcttcggt cctaagactc
     2281 agacagcgca gaggggaggt catctcgacg cgagccagta gagcaagtca caggccaaag
     2341 gcgccacagc gcggcctacg cgcaaatgac ccgaatccac ggcgaatttc ccctggcaaa
     2401 atacgtcagg gtcaatgacg acccgttccg tccgggtcgt cattgactgc ctgggtcgcc
     2461 ggtgactact tggcgagtcc aagccccgcg agaacttcga ttcaagcgcg agtcgagcgc
     2521 cctacaccga cttggtggtc acgacctcgc gctgttcccg cgacaagctc acccaacgac
     2581 cttcgatgcg gaagagtgcg ctcccagcgc tgtccatcgc gccttcgagc cgtccctgca
     2641 tgctgccttc tatcctgcgg cagcctcgga ctttgacgcc gacttcgcga ttcaatacga
     2701 gcaaacgcag cgtcgagccg gggggagaag gggcgcctgt ctcgagcatg acgttatcgg
     2761 cgtcggcacg aacgatgcgt gcgacacctc cacccactac gttgaagacg ggagttgcgg
     2821 gccgctcgtc agtcgattga gcttcggtgt tttcgctcat gcttgccggt ctttcgtgcg
     2881 cacctcagtc gcggcggcct ctgcggatcg ccgcgaccgc ctcgcgatcg gcctctttgg
     2941 tcttgatgct ctggcgcttg tcgtactgct tcttgccgcg ggccacggcg acttcgagct
     3001 tcgcgtgatt gttcttgatg tagcacttgg tcgaaatcag cgtcatcccg tcgcgctcgg
     3061 tgagcccgcg caagcgctcg atttgagcgc ggtgcagcaa cagcttgcgg ggacgggtgt
     3121 cgttgtgacc gaacgccgcg tgcttgagcg ggggaatgcg catgcctttg acccaggcct
     3181 ctccccgatt gtcgatgtcg atccaagcgt ccgaaagatc cgcggcgccc tcgcgcaagg
     3241 cgcgcgcttc actcccaatc aagaccagcc cggcctcgaa cgtgtccgta atctcgtaat
     3301 cgaacgtggc gcgccggttc ttcgaaacca agcgatcgcc caacgtcgtg gtcttgccgc
     3361 gggccatttt cagaggccgg cgttctacac cccggtcgcg tcacgtgcca gtctctcccg
     3421 gagtcggtga gataattgcc ggcggaacgg ggctggcgct tcaggccagg cgccactatg
     3481 cgaagggccg cgcatgctgc acacccggct cgaatccgct cgcacgcaca atctgaagga
     3541 tgtttcgctc gagctcgagc ccggcacact cgtggtgttg agcggggtct cgggcgcagg
     3601 caagagcagt ctggcgatcg acacgttgta tgcagaaggg caacgtcgct tcgtcgagag
     3661 cttcagcccg tacgcgcgcc agttcttgga gcggctggag cgaccgccga tgcaaagttt
     3721 ggagccggtg cccgccgcag tggcggtaga ccggcgtgca cccgtgaaga gctcgcgctc
     3781 caccttggcg acgctcgcgg atctcgagcc ctatttctcg gcgctgttcc tgcgcgaagc
     3841 ggtgcccgtg tgtccggagc accaggtgga gggcacgcga ttggatccag agcgcgcggc
     3901 cgcccgcgtc ggcgcggagc tgggcgtggg gcgcgcgctc gtgacctacc cgctgggtgt
     3961 ggatcgtgtg gagtcctatc tcggtgtgcg cgacaacttg gcgcagcaag gctatcggcg
     4021 gctctgggtg agcggtgaac tgctcgacat cgagcgggcc aagccctcac aggtgttggc
     4081 cgcgggtagc gaagtgggcg tggtggtcga tcgcgtcacg acgaccgagc gcacacgcgt
     4141 tgcgcaagcc gtggaacaag cctggcaacg gggtgcaggg gtctgcgtcg tgcgctccga
     4201 gcgtgagtcg ttcgtgttac gccaaggtct gggctgcggc gtctgtgggc gagagctgcc
     4261 cgagccgagt gcgggcctat tttcttacga gtctccagtg ggcgcctgcg cgacctgtcg
     4321 gggcttcggg cgcacgctgg gcatcgacct cgacaaggtc atcccggaca agacgctgag
     4381 cctgaaggcg ggtgcaatcc gcgccttccg cggtaagtcc acgagctacg agcgcggtga
     4441 actcgcgaag ctgtgccgtc ggcacggcat cccgttcgac cgaccttacg cggagctcgg
     4501 cgccgcccaa caaaggctcg tgctggaggg cgacggctcg ttcgaggatg gcctattccc
     4561 gggcgtgctc ggctggtttc gctggctcga gacacgcacg tacaagatgc acgtgcgcgt
     4621 cttcctttcg cgctaccggg cgtacgatct gtgccacggc tgcggcggac gtcggctcaa
     4681 cgcgaccgct ttggcgtttc gggttgatgg gttggacctg gcccagtggc acgcgctcga
     4741 ggtcggcgca gcattggaaa aattggcgca gctcgagacc cgctcggcgc agggggagct
     4801 cgcgcgcaag gagctggtct cgcggctcga ttacctgaag cgcgtcgggc tcgggtacct
     4861 caccttggat cgccaagctc gcacgctctc gggaggggag gcccagcgcg tgactttgac
     4921 ggccgcgctc ggtacctcgc tcaacaacgc gctgttcgtg ttggacgaac cgacgattgg
     4981 gctgcatccg agcgatatcg aaccgctgat cacgttgctc cacgagctcg cggcgcggaa
     5041 caactgcgtg ctggtcgtag agcacgatcc ggccgtgatg gcggccgcag gccgcgtgat
     5101 cgagctcggg cccggtgccg gtagcgaagg cggacggatc gtcggcgacg gtgtcccgag
     5161 cgcactcggg cgaggcaccg gggtcactgc gcaaactctg attggactac cgccgcggca
     5221 acggccgcct cgagcgtggg cacacgagct gcgtgtagtc ggagcgaccg agcacaacct
     5281 gaagttgccc gaggtgcgta tccctttggg cgtgatctgc gcggtgacgg gcccgagcgg
     5341 ctcgggcaag agcagcctgg tggtggacac ggtgtaccgc gcactcgccc tccgcgctgg
     5401 cgagctcgac gtggaagcgc ccggacacca cgagcgcttg gagggggcgg agcgcgtcac
     5461 gcgcgtcacg ctggtcgatc agacgccact cggacgcacc tcgcgcggta acgccgcgac
     5521 ctacaccaaa gcctgggatt gcattcgcac tctctatgcg cgggaaccga gcgctgcgcg
     5581 cttggggctg caggccaaag acttttcttt caacgtcgag ggcgggcgct gcgaggcttg
     5641 tgcgggtgag ggctacgaga cggtcgagat gcagttcctc tcggacgtgc gcttgatttg
     5701 tcctacttgc caaggccggc gcttcaagcc tcacgtgctc tcggtcgagc gtcacggcgt
     5761 cagcatcgcg gatgtgctga acatgaccgt gacggacgcg ctgcatgcgt ttcgcgccga
     5821 ggcctccgtt cagcgggcgt tgggaccgct cgctcggctc gggcttgggt acctgcgctt
     5881 ggggcagccg ctcagcacgc tgtccggtgg cgaagctcag cgcctgaagc tgtgtcgtgc
     5941 gctcgccgag ctgcggccgg gagagttgct cgtgctggac gagccgagtg ccggcttaca
     6001 cgtgagtgaa gtcgagaagt tgctcggggt gctcgatacg tgggtggact cgggcggcag
     6061 cgtgctgatg gtcgagcacg atctggatgt catcgccaac gccgactacg tcatcgattt
     6121 gggtcccggc ggaggccgcc gcgggggtga gctggtggcc tgcggcagcg tagacgagtt
     6181 gattgagagg ggttctcgca ccggcgaagc cctggcgcgg cgcttgaagc gtactgggcg
     6241 cacgcacggc ggagcgcgtg cgagctctcg caagccgacg ccgagcgtgc gctccgaggc
     6301 cgtattgcga gtggaacaag cgcgcgagca caacctgaag aatgtctcga ttggcttgcc
     6361 ccacggcgcc ttcagcgtcg taacggggcc gagcggttcg ggtaagagca cgctcgcttt
     6421 cgacgtggta ttcgcggagg ggcagcgacg gttcttggag actctgaccc cgtacgcgcg
     6481 ccagttcctg ccgagcatgc cccggcccga cgtggatcgg gtgagcggtg tgccgcccgc
     6541 gatcgcgctg ccccagcgca cggctcgcgc gggtgggctc tccaccgtgg cgacggtgac
     6601 cgaagtggcg cactacctgc ggctgctgta cgcgaaactg ggcacccccc actgccccga
     6661 gcaccaaacg cccatcgctc ggcggagctt cgatgagcta cgtgcggacg tgcacgcgca
     6721 acgcaagaaa agctacttgc tcgcgcccgt ggtccagcag cgtaagggga cctacttgga
     6781 cgtattctcg tccgccgctc gcgccggcat cctgcaagcg ttcgttgacg gcgcgctggt
     6841 gagcacacaa gaaccaccga agctcgcgcg cagccgtgag cacaccatcg acctcttgat
     6901 cgcttcgccc gcgagcccca agacgttcga accggccact ctgcggcgcg ctctggactt
     6961 cgggcgtggc agcttgaaat tgcgcaccga gctcggcgac gagctgttgt tttcgagtga
     7021 agcggcctgc ccgatctgcg gtttttctcc gcccgagctc gacccgcggt ggttctcctt
     7081 cgcgaccaag caaggccgat gtgaggcttg tgagggcaac ggcgtgctcg agacggagcg
     7141 cacggtcggg cgcggcaaga ctcgccgggt cgaggtggtg cgtcaggtct gcgcggcgtg
     7201 ccgagggcag cgtttgaacc ccatcgtacg ttcggttcgg ctcgacaccg agagttatcc
     7261 cgagttcacg gcgcgctcgg tgcgcgccgc gctcgaacgg gcgcgacgtc tgcgcttcga
     7321 tgcgagattg cagccggtgg ccgcacccct gatcggcgaa ctcacgcgac gcttgcagtt
     7381 cctgtgtgac gtgggtctcg actatctggc gctcgatcgt cacgcggcca ccttgtccgg
     7441 tggagagatg cagaggttgc ggctcgcggc gcaactcggc gcggggctca cgggcgcgct
     7501 ttacgtgctg gacgaaccca cgattggctt gcacccgcgc gacaccgatc ggctgattcg
     7561 gaatttgaag cagctcgtcg agctcggctc tacggtgctg gtcgtggagc atgatctcga
     7621 gatcatgcgg gctgccgatt acctgatcga catggggccg agcggcggcg cgggtggggg
     7681 aagagtggtg gccgcgggtt cgccgagcca ggtgctggcg ctgcccgact cagccaccgc
     7741 gcgcgtgctc gcgaacccgc cgtacattcg caaggctctg cccgtcaaat ctacggacgc
     7801 acgcttgctc ctttcggggg caacgcagca caatttgaaa ggcgtcgatt tgaacctcgt
     7861 gctcgggcgt ttcaacgtcg tctgtggggt cagcggctcg ggtaagagca ccttggtgag
     7921 tcacgtgctg ttgccggcgc tgcgccgcgc gctcggattg gtcgcggaag aaccgggcgc
     7981 tcacgcgggg ctgacgggct tcgatgcgct ccagcgcgct ctgtccgtgg accagtctcc
     8041 gatcggacgc acgccacgct cggtgccggc gacgttcctc ggcatctggg acgagattcg
     8101 gcggctgttc gcggctacgc cggaagccca agttgcgggt ttcaacgcca cgcgcttctc
     8161 cttcaacagt aaacaagggg gcgcctgcac cacctgcgat ggccagggct ccatcacgca
     8221 cgagatgagc ttcttgcccg acgtcgtcgc gccctgccca acttgcgagg gcttgcgctt
     8281 cgagccgcgc acgctggccg tgcgttacct cggcctcaac gtgggtgaag tgctgaagct
     8341 caccgcagag caggcgtgtg agcgattcga gcatcaccct cggctcaagg cgccgctgcg
     8401 cacgctcacc gagctcggcg ccggttacat tcaactcggc cagggctccc ataccctttc
     8461 gggcggcgag gctcagcgct tgaagctggc cctcgagttg accgcgggcg cccgccacga
     8521 acgcactctt tatgttctgg acgagccgac caccggtttg catccgagtg acgtcgagcg
     8581 gctgctctcc gtactcggtc gcttggtgga gcgcggcgac acattggtcg tgatagagca
     8641 ccatccacag gtgatcgcga gtgccgattg gatcgtagag ctcgggccag aaggcggaga
     8701 gaatggcggt aaaattgtgg cggagggaac agcgcgcgag gtggctcgcc gcaagtccgc
     8761 caccgccagc gtgatccaac gcattctgtc tttatgattg atcactcgat cggttgcgcg
     8821 gacacgcacg ctcggttgcg tctttttcag ctagttcccg cgaactgaaa tagattttcc
     8881 actcttcgtg attggcaatt gcgagtcgaa taatcgattc ggtgattgct tggtttccgc
     8941 ctgatcggtt actgctctca gcactctaac caggtctcga accattcgtg actcacgagc
     9001 ggaagtcacg atgagcggag aatcatgaaa agtacacgaa cgctgttcat tgggttgacc
     9061 gcggtaatct gcagtttggc tgtcgcgtgc ggcagcgatg acgacgacgc gggtcccgcg
     9121 acgggaggta actccgcagg gtcgtctgcg aagggtggca acagcacgag tggtggcacc
     9181 tcatcgactg ccgctccgag cggtggacga tcgagcggcg gcaccacgag ctccggcaac
     9241 ggcggcacga gcgtgggtcg tggcggcgcg acacaaaatg caggcggcgc ttcggaagcc
     9301 ggatcagaca ccggtggtgc gggacccgag gcgggtgcac cgagtgaagc cggagcgggg
     9361 cccaccggcg gtcgcgtagg aatgggcggg cgcagcggaa gcggcggtcg cacgggcacc
     9421 gcgaccggtg gtcgcacctc gagtggcggc gcttcgagcg gcggacgcac gagttctggc
     9481 ggcatgaccc cgggcggtgc ggcgggtgaa gcgggtgcgg gcggtgccag cgcggacgca
     9541 tgtccagaaa ctcgcccacg gcgcggcgat ctcgattgct ccgatcccgg cttggtctgc
     9601 acctacggtc aggtcagctg cacctgtgaa cgtgcgcgcg gcggtgcaca gttctcctgc
     9661 tccactactg agacgacgac cggaggtcgt tcgggcacgg gcggcgcgag ctcgggcggt
     9721 acgagcaaca ccggcgggcg acgtggtcgt ccgacgcccg cggccggcgc cgagggttga
     9781 gtgattcgcg cgggcctggt cgctacgatc gggcgccgca ctgggtggca ccgcgtgccg
     9841 ggcacgtgcc cggcacgctt gctcccgaaa ccatgaatag cccgcggaag ttcgggaatt
     9901 tcgcggatta cgttcacggt acgtagcttg ctgaggtgac gcacgacggc ttcgtcggag
     9961 agttcgcgat tgcgcgatct cgatggcgaa ggcgcgagtc aacggcgagc gcgtggtcat
    10021 cggattgacg atgaatcggc ctcgccatcg cgaggaagat catgcgcaaa tcagtgtggc
    10081 tacggagtgc tgtgttgagt ctcttcgtgg cgagtgcttg cagttccgag aaggacccgc
    10141 cactcgccga gaatgcgggg aagggtggag ccgcttcgaa cgcgggcggc acgcgtgcgt
    10201 cagggggggc cccaaacgcc gggaggacga ataccggcgg cattggtgac gcccgcggcg
    10261 gcgacactta tggcggcact tactacggcg gagccgcctc gggcggcacg aatgaagccg
    10321 gaaacgggaa tgcgggcgaa gccggcgaga atgccggagt ggcgggctcg ggcggtgagg
    10381 cgggcgccag cgcggcgctg cgtgatccga gccatggcag cccgattgcg ctggcccccg
    10441 atgagcgttt ggccgtagtc gtcaatcgcg atgtcggctc tgtatccatc gtggagctct
    10501 cggcgccgga agccgggctc gttcccgaag tgcgcctgaa gcgagaactc tcgctcggag
    10561 cgggctcgga gccctggcag gtcgtcatct cgccggactc ggacactgcg ttcgtcgtgc
    10621 tgaagcgcga ccagcgcctg gtccgcattc gccacctgca cggggagccc aagctggacg
    10681 gtaccgtcag cgtgggttcc gagccgacca gcgtcgcgtt gagcccgagc ggagccttcg
    10741 tgttcgtcgc gaacttcgtc gatggcacgc tcagcgtgct gaacgccgag acgctgcgct
    10801 tggtgcgcac ggtggacttg aatcgagcct tggtcgacgg tggttacctc ggccaagtcg
    10861 aggcccgccc tgcgcttgca cacccgcgat ccatcttggt cacgaacaat cgcgacgatc
    10921 aggacggcga tgaagccgtc tacatcaccg agtactttgc ccaacagcta gagcccgaag
    10981 ccgcagatgg ctcgaacgcg gatactcgca aggcaggtct cgtgtatcgc gtgtcgctct
    11041 cggactacag cgtccagacc atcgaacttg ccgcgctcgc cgacatgggt ttcagagccg
    11101 gcgacgggaa ggcggcgggt tgctatccca atcagctgca aagcatcgcg gccgcgggca
    11161 agttcgctta cgtgctttcg gtttgcgctt ctccacgcgg gccgctcggg gtgcgcgtca
    11221 ctacgacggc ctgcaagacc gtcgccgact gctccggcct gggtctggtc gagccgacgt
    11281 gtgcgctgcc ctttggcggt gctccgagcg cgctttgcac ggacgtagcc tcggtgaaga
    11341 cgacgacggc tccggtggtg tccgtgattg acttggagca aggtcgtgaa gtttctggct
    11401 ctgcgcgcaa cttgaacgct gaattcgagt cgctattcga tgcgcgcggc acgcccgcga
    11461 ccgaccggcg cttgccactc tttgcgagtg acatgagctt cgtgccggac acgtcggtgg
    11521 cctacgtgtc cgcgaacggt acggatgccg tatttcgcct cgttttcgac gccgcgaagg
    11581 gcacgctcac ttccgtcggc gcctcgacgc agagcttcat cgatctgttc ccgagcggca
    11641 tcgcaaagga gcgcgcgggc aagaacccga tcggagtcgt gagcggcggc aacacgcgca
    11701 agtatgcgct cgcgctgaat gacgttggcc gcaacctgag cgtgatcgat ctcaacgcgc
    11761 aggcgcttgc cggtggcgtc gccaacgctc gcgtgattgg cgtggccgat cagcccgagc
    11821 gcggctccga cgccgaacgc atcctgcgcg gcaagcgctt cttcgatacc ggagtcggtc
    11881 gatggtcgct caacggagaa ggttttggcg cctgccaagt ctgtcactcc gatggcttga
    11941 cggacaacgt gacctggtat ttcgcgcgcg gcccgcgtca ggccaccagc ctggacggga
    12001 gcttcgcttc caatgatcct accgaccaac gcatcttcaa ctggaccgct attttcgagg
    12061 acgttcccga tttcgaggcc aacactcgag atatttcggg cggggtgggt gcgattgtct
    12121 cgaaggtgag tgccccgccg cagaactcgg atcggatcga cttcgtcggc ctcaaacatg
    12181 cgggtttgaa cggctcgtcg gcgcaagccg cggatccgaa gaatccgctc gggttcgcgg
    12241 agccaccgct gctcgaagat tgggccgaca tcgagcgcta catccagacc atccgttcgc
    12301 cccgtgcgcc ctcgacgctc ataccttccc aggtcgcgga aggggaaaaa ttgtttgtga
    12361 gcgccggatg tcagggctgc cacggcggcg ccaaatggac ggtctcgcgc cgcttctaca
    12421 cgtcgagcac cgagacgaac gctcgcctcg gcaccacacc gttcgagatc ccagccggct
    12481 tcccgcaaga gctcctgccg gctcgtcaga aagccaatca gctactgcga ttcggtggca
    12541 gtaaccccgc ggcgttggat cagatcttgt gtgccatacg cccggtgggc accttcgatg
    12601 tggccgaggc tggcgtgggc atcgccgagc tacgcgcaga catgaagacg gttgcacaag
    12661 gcgatggcga tcccgctggc gaaggacgag gttacaatcc accctccctg ctggggatga
    12721 cgttgggcgc gccctacctg cacgccggct ccgcgcgaac cctcgaggcc ttgttcgcgc
    12781 cgcctttcga gcagcattat cgtgcgttgt cgccgaactt cctcgccgag agcgatccag
    12841 ccgtgcgtca gcggcagatc gacgcgctgg ttgcgtacct gagcgcggtc gatgaaggca
    12901 cgccgatcgt ggatctgccc aagccagggc cgctgggcgg ctcattgtgc cccgaatcgt
    12961 tctaggaggc tcccgatgct gtcgcgctca gcctagtgcg ctcgcacgca agctcaccgg
    13021 ctcaacggcg ggcaccttga gggagcgctt cggatcgagt gcgactagcg caagctgacc
    13081 acaagccgcc gcgatgtcgg gaccatgtga gcggcgagcg aagcagcggt agccggcgtc
    13141 gagcaaccga cgttgaaagg cccgtacccg ctcggggcta ggcgctacga acgcgctctg
    13201 cgcgtgtgca ttgtagggaa tcaagttgat gcggaccggt aaatctcgca ggagcgagat
    13261 caaacggtcc gcatccgcgt ccgtatcatt cacgttcgcg agcagcacgt actcgaccag
    13321 cgtgtcccgc gttttgcagg tcgccgccgc gtcctcgcgc agtacctcga gtaacgcggc
    13381 gatcggccaa gtacggttgt gcggcatcaa ccgttcccgt tgttcgtcgg tggtcgcgtt
    13441 caaggatagg gccaaagatg ccttgcattc tcgaagaaaa cgccgcattc caggcactac
    13501 acccgacgta gagacagtaa cggagctgag ccgaagcccc ggtgcggggc cttgattgag
    13561 cagctccacg gctctcaaaa cctcctccaa gttgtccatc ggctcaccca tgcccatgaa
    13621 cacgacgttc ttcgcaggtc ggtctggcgg cgcttgcatc cgacaggccc agtactgcgc
    13681 gagcatttct tcggcccgta gcatgcgctc gaagcccagg ctctgcgtgg cacaaaacgc
    13741 gcagcgccgc gtgcagcccg cttggctcga gaggcaaacg gtgctgcgct gactctcttg
    13801 gatcagtaca ctttcgacgc tgctcccggc gagatcgaac acgaacttgc gagtcccgtc
    13861 ttggcttcgg tgttcgctca ggagcttggg cacgagcagg ccgtgccgct cggcgaacgg
    13921 ccggaacacg tcgcgcgcta cattgggaat cgagctcgga atgcgctcgg gcaggctgct
    13981 cagaccatac aaccacttca gggtttggat cgcacgtgtg cggctgccga gctcgctcgc
    14041 gagctcactc aacaacaagc caggtaacgg gcgcgtcacg ggcggcacgc tagcacgcgg
    14101 cgtcatcaca cggcatgccg cattcacacg gaggcgcgga cccagcgctg gcgaaaggcg
    14161 gcattgcggg ccgccgcctc ttgcacgcgc gc
//
LOCUS       DASTKU010000112         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_468319, whole genome shotgun sequence.
ACCESSION   DASTKU010000112 DASTKU010000000
VERSION     DASTKU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468319"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..978)
                     /locus_tag="VFQ61_03965"
     CDS             complement(223..978)
                     /locus_tag="VFQ61_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953632.1"
                     /translation="MSHERTRLIGVVALVYLLFSSLAFNARASDFEPIQLPQTATEPG
                     VVIYRARSARSAPIAVLLHGMCGEPQRLCAWFAEAITRSEHLICPRADAPCAGGGVSW
                     SSRGLETRVEAAIERARSALGPAMDLRRPRTLIGYSLGALRAVELAETSPETYERLML
                     IGARVNLDPARFRAARNPRVLLAAGRWDMMYGAMQNEARRLTRAGFTARFWDLGQMGH
                     PLSPTLPAILPLGLDWLFEDGVASPSRPQPALQ"
     gene            complement(1178..2155)
                     /locus_tag="VFQ61_03970"
     CDS             complement(1178..2155)
                     /locus_tag="VFQ61_03970"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HET9953633.1"
                     /translation="MPTPVPVDTTTRLADPKLRQELRAAVARRVRSEEVDDILHATFA
                     DVLASRRAPEDAEQFRRFVFVVARNRVMDYFRRRGRELPDESVSDFPAGPFSGEATSH
                     HELLSAQDMLRWVEGNLPDEEAKHTLEWMLREQDGERLEQIAKDEQMPAPRLRQRVSR
                     LRRFLRERWAAEWALGGLVAVLGLGALWYWQRRTSAEVVQPERVSEQVEYARRLRHGV
                     MRDCHSGEARRCLEELNRAKRLDPAGDTRPEIVEARRIAGERLAPVPDNTAPDPIAPA
                     PSSLNPKVSPSFKQTAPRATAPNGSAPAPKAPTLPNWGSDSIERKPERK"
BASE COUNT          420 a          764 c          752 g          453 t
ORIGIN      
        1 aagccgatca cgatgacggc aaatagaact ccaatccagg tcgacatgca gagaactcgg
       61 agcgcgatac tacacgaatg aaagcctcgg ctcgacgtgt atccaaggcc ttgtgccgtt
      121 ctgcctctca ataccaatca aggcgagcgt gacccaacgt tactcgttct ttgccgccgg
      181 tccgtgcagc ttatacaaag tgggagaaac gccagtccgg gactactgaa gtgccggttg
      241 aggccttgat ggactcgcga cgccgtcctc gaacaaccaa tccaaaccga gcggcagaat
      301 ggcgggaagg gtgggggaga ggggatgtcc catctgaccg aggtcccaga agcgcgcggt
      361 gaatccggca cgagtgaggc ggcgagcttc gttttgcatg gccccgtaca tcatgtccca
      421 tcttcccgcg gcgaggagta cgcgcggatt gcgggcggcg cggaaccggg cggggtcgag
      481 gttcacccgg gcaccgatca gcatcaaacg ttcgtacgtc tcggggctgg tctctgcgag
      541 ctcgaccgcg cgcagagcac ctagcgagta cccgatgagt gtgcgggggc gacgaagatc
      601 catggctggg ccgagtgcgc tgcgcgcgcg ttcgatggcg gcttctacac gcgtttctag
      661 gccgcgcgat gaccaactca ctccgccgcc cgcacacggc gcgtccgcgc gcgggcagat
      721 caggtgttcg gatcgcgtaa tcgcttccgc gaaccaagcg cacagcctct ggggctctcc
      781 acacatgccg tgcagcagaa ccgcgatcgg tgcggagcgg gcactgcggg cccggtagat
      841 gacgacaccg ggttcggtgg cagtctgcgg gagctgtatc ggctcgaagt cagaagctct
      901 cgcgttgaat gccaaactcg aaaacaatag atacactaag gccaccacgc caatcaggcg
      961 tgttcgttcg tgtgacatga ctcgcacctg cgagcgagtc gggaatttcc tctatcgatt
     1021 gctcagttcg tcgagactcg agctcaccgc gaatgggcgc gatgcggctg cttcgcgcgg
     1081 tagagtgacg tcctgtggct gatgctgagt tgttcgaggg cttgtcggct cgctccagca
     1141 gttcagcgtg atgcgagcgg gaatgtgacg cgcccgccta tttccgttcc ggtttgcgct
     1201 cgatagagtc gcttccccag ttcggtagcg taggagcctt cggcgcgggg gcggatccat
     1261 tcggtgcggt cgcgcggggc gcggtctgct tgaaagaagg acttaccttg gggttgagag
     1321 agctcggcgc gggcgcaata ggatcgggtg cagtgttatc cgggacgggc gcgagccgct
     1381 ctccggcgat gcgccgcgct tccacgatct cgggccttgt atcacccgcg ggatccagtc
     1441 gcttggctcg attgagctct tccaggcagc gtctcgcctc gccagagtgg caatctctca
     1501 tcactccgtg gcggaggcgc cgcgcatact ccacctgttc ggaaacgcgc tccggctgga
     1561 ccacctctgc cgaagtgcgt cgctgccagt accaaagcgc tcccaacccg agcacggcca
     1621 cgagcccacc caatgcccat tccgcggccc aacgttcgcg cagaaagcga cgcagtcgcg
     1681 agacccgctg gcgaaggcgc ggggccggca tctgctcgtc cttcgcgatt tgctcgagcc
     1741 gctcaccatc ttgctctcgc agcatccact ccagcgtgtg cttggcctct tcgtcgggca
     1801 ggttgccctc cacccagcgc agcatgtctt gcgcggacaa gagctcatgg tgggaagtgg
     1861 cctcacccga gaatggcccc gcggggaagt cgctcaccga ctcatccgga agttctcggc
     1921 cgcggcgtcg gaagtaatcc attactcggt ttctcgcgac cacgaacacg aaccggcgaa
     1981 actgctctgc gtcctccgga gccctccgac tggcgagcac atccgcgaag gtagcgtgca
     2041 gaatgtcgtc cacctcttca ctccgcaccc gacgcgccac cgcggctcgc aattcctgcc
     2101 gcagcttggg atcggctaga cgggtcgtcg tgtcgaccgg aaccggggtg ggcatcgcca
     2161 tctctgcttc tcccatcgct cgaccctcgt ccagtgtgac cgacccggcg aagattcttc
     2221 ctgccggccc tggcagcgtt tacagccacc ttgtttaagc aggccttcca tcgcttgtcc
     2281 ccgaccgaga acggtgaaca aggagccgct ctgcttgcgg tcgagctctg cttgcggtcg
     2341 agctctgctt gcggtcgagc tctgcttgcg gtcgagctct gcttgcggt
//
LOCUS       DASTKU010000113        13532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1136355, whole genome shotgun sequence.
ACCESSION   DASTKU010000113 DASTKU010000000
VERSION     DASTKU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13532
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136355"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(938..2112)
                     /locus_tag="VFQ61_03975"
                     /pseudo
     CDS             complement(938..2112)
                     /locus_tag="VFQ61_03975"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2699..3721)
                     /locus_tag="VFQ61_03980"
     CDS             complement(2699..3721)
                     /locus_tag="VFQ61_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953634.1"
                     /translation="MKPIAFFSALALLPALNAHAQPPAEPQPQTTTSESHLEATTPSS
                     ATVAPSQSQTNAGPAPTAAPQPVTGGCELGPHAGIPEEDAQTAARLVCEELLRQKAPT
                     DQNYRVELLKLGDAIFLSVAAIRPDAPGEQRRVRLANIEEAPDATPRLVTALLQHKDV
                     GNLQEYGNLLQSETRAPLRKSGALLVGVGVMGQYSNHIVAPGFELSLRYDTPDWVIGS
                     ALRYSGGSTKERHSEIFSWGVGGRYMFTHSDAAPFAGAGLAFETITASQERTSGNSTE
                     TVFRGDRSGLAVYGEAGVEFLRTHKSRLAAAIRVDAPIFNVSTAYGTNTRYYLPTSFT
                     LSYSFN"
     gene            4260..4466
                     /locus_tag="VFQ61_03985"
     CDS             4260..4466
                     /locus_tag="VFQ61_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953635.1"
                     /translation="MKKTLGLLLICSAAAGVLQGCAYAGVAASGERAVVARNDGFLFG
                     ILRKVYVCKISDAGLTACQDSDSP"
     gene            4463..5029
                     /locus_tag="VFQ61_03990"
     CDS             4463..5029
                     /locus_tag="VFQ61_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953636.1"
                     /translation="MSDSGGGVPGLGPIVARDLGAPARGWIRAGVLMGCILGLLGCKR
                     ARNEASQSTPVVSATTSAAQDASPQVSAGYGEPVGARVEASAMTPAFEVAVASSGEAV
                     GTFVSPLTALVAQSLRDCGGLRAVAESKPALSLAFSVVAGRAQADSIQAPALPEAELG
                     CVSRGLLGQKFPVLPAPQKFLLQFRLTS"
     gene            5303..5833
                     /locus_tag="VFQ61_03995"
     CDS             5303..5833
                     /locus_tag="VFQ61_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953637.1"
                     /translation="MSTPSDAATGSAGSSAASVAASGPASTASEPLPSLDSGAVAPGH
                     FKPAYTTWDANVEGGYGRYFPDKSGLGFVRGRAGVLFAHDPAYYALGVTYEWSNQAPW
                     AFGVQAEMLRIETGLWAQAGVLIDSNIKAGATLALGWSIFGVEGQVRSFDPEPYGLAV
                     LGKVRIPISFIAMAFK"
     gene            5974..6939
                     /locus_tag="VFQ61_04000"
     CDS             5974..6939
                     /locus_tag="VFQ61_04000"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET9953638.1"
                     /translation="MIKNLIVISAAWFSACTLGKASPPPDTYQGVVELEETQVAFETA
                     GRVRRLTVEKGDLVQPDQLLAELDDELARVSRSGRVMEAEAARSQSALVQARAKAEDI
                     AALAARVRAAQAAEALLTKNLARQKALFESSAVSAASLDELTGQLDRARADREAAQAQ
                     LAALERGARREERDVALARAEAAEAALSLEDERIARMTLKAPRKGRVLDRFVEIGEVV
                     AGGAPVYTLGDTERPYVDVFVPQGQLSGIQAGSRAELHVDSETRSFSGVVEHVSRRTE
                     FTPRYLFSEQERPHLVVRVRLRFQDPGERLHAGVPAFVHIARDQP"
     gene            6936..7913
                     /locus_tag="VFQ61_04005"
     CDS             6936..7913
                     /locus_tag="VFQ61_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9953639.1"
                     /translation="MTEGAIPDESSLVIETERLGRRFGSLVAVRDVSLAVRKGEIYGV
                     LGPNGAGKSTTIRMLCGILDPSSGRGRVVGYDIVKEPERIKQNIGYMTQRFSLYEDLS
                     VEENLNFYAGIYGVPWLTRKQRVSSVIERAGLSDRRKQIAGTLSGGWKQRVALASATI
                     HEPPLLFLDEPTAGVDPVSRREFWEQIHRIAAEGTTVLLTTHYMDEAERCHRLAFIFG
                     GRLLGVGTPEEMTSRAGIAMLELVVSDPIAAAAALRRRPAVEEVSHYGHVMRVAVNAR
                     VEPEPHRLVRESLASAGITLQSIQESRVTVEDAFVATVRREGQAAGGAS"
     gene            7910..9034
                     /locus_tag="VFQ61_04010"
     CDS             7910..9034
                     /locus_tag="VFQ61_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9953640.1"
                     /translation="MIARPLVIAYKELLQLRRDRLTLAMMAMLPLLQLVLFGYAINTD
                     VRHIPTVVYDSDYSAASRDLSRSLEATGFYDIVGHVHGPTEVESALRSGRAKVALVVP
                     ANYARDLVRGRRAGIELIVDGSDPQTVASASNTAASLVAARSSELLLSRVKRAGLPGF
                     EPIGLEPNIWYNPDLRTAVFVVPGLVGVILTMTMVMLTAMAIARERERGTLEQLIVSP
                     VKRVELVLGKIVPYILIGYLQMSLILLLGRLLFQVPLAGSLALLYALSFLFISANLAL
                     GLFFSTLAKTQQQAMQMSFFFLLPNILLSGFMFPFEAMPAAARGISQILPLTHFLRIV
                     RGITLKGSTFEDLRAELVWLACILVALITLASLRFTKKLV"
     gene            9051..9665
                     /locus_tag="VFQ61_04015"
     CDS             9051..9665
                     /locus_tag="VFQ61_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9953641.1"
                     /translation="MPRPKSDIDTRIVHAARERFLQEGVDGASLRAIAKDAGTSIGMV
                     YYYFPTKDDLFLAVVEEVYERVLADMAEALSPSLPVATRLSRLYARVGQLTEEEEITV
                     RLVVREALVSSARLQRLIERFQRGHIPLILRTVADGIADGTLAPNRHPLLLMAATLAF
                     GAAQQILTQVLGPRLGPALALPPAQELPGQLVDMLLHGVGSKRG"
     gene            complement(9682..11907)
                     /locus_tag="VFQ61_04020"
     CDS             complement(9682..11907)
                     /locus_tag="VFQ61_04020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9953642.1"
                     /translation="MSTAVLRRETESDVRVTARTLRERSQMGAIIVAFLALAVAATTR
                     FRLHHPWATTSALVLLLGCAVLRLTMQGSEGESEHHARQWLRRFRIGALFSAGIWGSS
                     AAAAAGQQLDADTLTLLVPTAGIVAGATSSLSPDLRLVRAYLLLMLVPVISSVALRWG
                     LHGAGVSGSICLFLLYSWLQAAHQHRSFWAAVHQNVELRAHANALELARSAAEAADRR
                     KSEFMAYVSHEMRTPLTSIIGYAELLEKELPPSAGEDLRVISRGGRQLLEIVNQLLIF
                     ESLEKSGLELESREFQLGEVLSDVRDLVQVQATSRGIDLHVEPTASTIIVGDPGRLRQ
                     ILVNLVANALKFTDHGGVRVQSRLEGDAPQALLVCEIIDSGIGMSQEAVAQLFVPFKR
                     VENASNGTREGTGLGLAISQRLARLMGGDITVQSQLNVGSTFTLRVPLQVSSNQVARQ
                     SAAGEGREGATSLAGVKVLVVDDSPEIRSLLSAVLGRAGAEVHCADDGADAVLAALRT
                     HYDLVLMDVRMPGLDGCDAVSALRTGGFTRPIIALTGDVSESGREQYLDAGFNWVVCK
                     PIDATAVVRCLADFHLHGYPSQATLAYKELQQSGPMPCARGEIRPKRSLLSQRPELRE
                     FVLEFIEDLRHRITDLQQASENSDREQLLALSHRLAGSGGTFGFQDITDTARQLERML
                     RAEAADPRRIPTAVQELIRACSAACEAAIRDAAASEALARPSESTSPVAFAEPARSSR
                     G"
     gene            12155..12724
                     /locus_tag="VFQ61_04025"
     CDS             12155..12724
                     /locus_tag="VFQ61_04025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9953643.1"
                     /translation="MFKLQVIAVSTREGRAGLPLVQWFSSRAMAHGGFDVELVDLKDV
                     ALPMFDEPAHPRLKKYRGEHTKAWSARVDAADAFVFVMPEYNHGAPPSLINALDYLSS
                     EWAYKACGFVSYGGLSGGTRGVVHAKQVLSAMKMVPLVESVAFPFFNKLFREDGSFDG
                     SQQEAAAKTMLDELVRWTQALTTLRAPRG"
     gene            complement(12755..13246)
                     /locus_tag="VFQ61_04030"
     CDS             complement(12755..13246)
                     /locus_tag="VFQ61_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953644.1"
                     /translation="MPANRSEENFPFSTRPARAPSHTIQACVIVWLALLGACGSEPES
                     VGAASGGQSGHGGDRQPSSGGRTPSLVLCAAQAPSSCPEPAPSYRADIEPIFQRSCVV
                     CHSGTKGGPWALTDYGHVATWRDTVRTALLECSMPPPDSEVSIEAQESQLVLTWIRCG
                     MPP"
BASE COUNT         2371 a         3933 c         4592 g         2636 t
ORIGIN      
        1 agttgaatgg gcggagagtt gaatgggcgg agagttgaat gggcggagag ttgaatgggc
       61 ggagagttga atgggcggag agttgaatgg gcggagagtt gaatgggcgg agagttgaat
      121 gggcggagag ttgacgacaa attgatttgt ttatgggcag ggtcgtgcta gattggtggg
      181 gtcagtaagg aggaatctat gtgtgtttgg cgggtgggac gagagcagtt gagtctcggg
      241 ttcaagggtg cttggggcgg gaggagacca ggcgcggggc gtaagcggaa cgggagcgag
      301 atccgagtgc gacacgtgag tcgaccggct catcgccgtt accacccagt gcacatcacg
      361 gtgcggtttg cggtggggtg tatgcgttcg caggtggcgt ttggggaggt gaggcgtgcg
      421 attggcacgg tgaatgcgag gaatgaagct gcttttcgag tggtagaatt ctccgtgcag
      481 gccaatcacc tccacctgat tgtggaggca gaagggaggg cagagctcag tcggggtatg
      541 cggtcgctca actcgaggat tgcgcgacga gtaaatcgcg tactagggcg tagagggcgt
      601 cttgtttcgg accgtcatca cgaacgcgct ctctcctgcc cgcgagcggt tcgccatgcg
      661 ctctgttacg tgttcgcgaa tttcagaaag catctcaccg tggaagggcg tcgtcgtgag
      721 ccactcgacc cgtgttcgtc tgctccttat ttcaacggct tctctgagtt gccgaacgcg
      781 gcgttggacg gaggctcggc gctggtaaga gcgcgcactt ggctattgag ttgtggttgg
      841 aagaaacatg ggctcatttc gatttgggag gcgccgcgcg gcgcagattc gtaatcagcc
      901 aactcgccgt gcaggttttc tctggtgata gcgactatca atgtatccgt ccggcctgcg
      961 ccgtacttgc gttagcccgg aggcggtacc cagttccgag gcagaagccg gtcgatgttc
     1021 gcggccggcg tggagccaca cggatgagca cgtcagacag ataagcgagt gggtcgacac
     1081 cgctcgtcag gcacgtggct acgacggtgt agagcgcggc gaggttctcg ccggcttctt
     1141 tgctgccgac gaagaggaat gttctgcggc cctgagccac gacgcgcagt gcgcgttcac
     1201 tgacgttgtt gtcgggggcg atgcgaccgt cggtgatgaa gcgcgtcatg gtctgccagt
     1261 tttttttcta tggcataccg gatggccatg cccatggggg actcttgggc aggtgcagtg
     1321 gtttctgctg ggctagccag acatagagct tgtccataat gggcaggctg tgctcgagcc
     1381 ggatgcgcgc gtgctcctcg ttgccgacaa ttcgagcctg cttcacgtcg tgttcgatga
     1441 cgtagagttc acgtacgagt tcgagcgcgg tcttggcttc aggtgctgag gattgcgcat
     1501 tgaagaagcg tcttcgggca tgtgccaggc accccgcgcg ttgccgtccc tgcacgccgg
     1561 tcaccgagtt gtaaccagtg aacatatcga cgacgagcgt gcccgtggtc ccccccagga
     1621 cttctttcgg tgtgtcgcca ctccgagaca tgctgaattg gtatgcaatc aggtctccgg
     1681 acacgaaggt ccagatatac ggcttcttgc gggtgtcctg caggcggtgg gcgtttcatc
     1741 cgcatggacg atctcggcct gcgcgaccag cgccaggatg cgacgagcga acggagccaa
     1801 cacctcccct gctcgatgaa agagctcgtt catcgtgctg cgcgccatcg gaataccgat
     1861 gcgccgaaac tgctgctcga tgcggtaaat cgggagagag tcgcagcact tgctcacgac
     1921 cagatgcgca atgaagccag ggccatactg cgtcctgtcg aataccttgt ctggcacggg
     1981 tgcggtcacc acatattggc cgcagcgaca ggcgagcgtc tcgcgcagac gctcgacctc
     2041 gcctctgcca cgatcttcga ccagaactcc gcgtcttttc tcgtctgtgc catgccccga
     2101 cacttcgctc acttggacgt aagacgggga gtgcgtccat ggtcggacgg atacctatcg
     2161 attacgcccg gggccaccgt agaactacgg aactcgcggc ctcgctcggt gtagtcctgg
     2221 gttaggtaca acagagccgc ctccactcgt ggcgtctccg cgcgccgtag tgccgccggt
     2281 ggacgtggtg ccagcgctgc ctccgttcgc cgtgatgccg ccagcggtgc gtgcgccttc
     2341 gcttgggccg tcgtcgccgg taatggccgg aacgcagctc cgcccggcac gccgttgggc
     2401 ctgcaaagaa ttcgcttcgt gagagaacgc gcgactcgcg atggagcgag gcagttcgcc
     2461 ttcatcgggc agatcgaggc atttccggtg gaggcgatcg gccgagtgct catctgaagc
     2521 ctgcccgagc gaaggcgggc gctcagcttg ctacgactgt cgcagggatg caccggcgca
     2581 gccgcgagag atacggtagc ccgcgtgtgc ccacggcgct cgcacgtgac gagcccggag
     2641 caccgcgcct cgagttgcac ttgtgatcgg gatgattgat gtttgaggcc ggcaagtgct
     2701 aattgaacga gtacgagagt gtgaaagagg tggggagata atatcgagtg ttggtgccgt
     2761 acgcggtgga tacgttgaat atcggagcgt cgacgcggat ggcggcggcg agccggctct
     2821 tgtgggttct gaggaactcg acgccggctt cgccgtacac ggcgagcccg gaacgatcgc
     2881 cgcgaaacac ggtttcagtc gagttgcccg aggttctttc ttgagacgcc gtgatggtct
     2941 cgaaggcgag ccccgcgccc gcgaagggtg ccgcgtcgga gtgcgtgaac atgtagcggc
     3001 caccaacacc ccaactgaag atctccgagt ggcgctcttt ggtcgagccg ccgctgtagc
     3061 gtagagcgga gccaatgacc caatcgggag tgtcgtaacg taagctcagt tcgaagccag
     3121 gagccacgat gtgattcgag tattggccca tgacgccaac gccgacgagt agcgcgcccg
     3181 acttgcgcag gggagcacgg gtctctgact gcagcaagtt gccgtattct tggaggttgc
     3241 ccacgtcttt gtgttggagc agcgcagtga ccaggcgcgg agtggcgtcg ggcgcttctt
     3301 cgatgttggc gagtcgaacg cggcgctgct cgccgggagc gtcagggcgg atcgcggcca
     3361 cggacaagaa gattgcgtcc cccaacttca acagttctac tcggtagttt tgatcagtgg
     3421 gtgctttttg gcggagcagt tcctcgcaca ccaagcgcgc cgccgtttgc gcgtcttctt
     3481 caggaatgcc ggcgtgtggt ccgagttcgc agccgccagt caccggttgg ggtgcagccg
     3541 ttggtgcggg ccccgcgttg gtttgcgatt gcgacggggc gaccgtcgcg ctgctcggag
     3601 tagtcgcttc gaggtgcgac tcggatgtcg tggtctgtgg ctgcggttca gcgggcggtt
     3661 gcgcatgcgc gttcaaagcg ggaagtaagg caagagcgct gaagaatgcg atgggtttca
     3721 ctgtgttttc ctcccaaacc gagggcaatg gacctagccc cgaactgcca tctggcgtgc
     3781 ttggtcgaag gactacgcaa cacgcgtgcc atgacgctcg tgcccgcaaa accgagcgga
     3841 tcacgcttaa ccatggctaa ccaaatggag cgtcgacgtc cgtggttcac acagagtcga
     3901 tgttttgcaa cacgcggagc taatcgcctc tctcccaatt cgagctagct ctgatctact
     3961 gagcgaggct cggacgaccg gccatcgaag aaatgcctgt gactcagccg atcgggggac
     4021 gcactactgt tcaaaaatgt cgggaagaag tgtcatggaa gacccgtacc tcgcccaccg
     4081 ggacttgact cacggtctgc tagcgtggtc tacccgggtc acggtgagca tgagcgtttg
     4141 gggctcggtt gttactcatc aactggccaa gcggcacggt taggccgcgc cgtgagtgag
     4201 tcaggtgcga gggctagagc tcgattcgtt gaggctgacc agaggagaga ggactgaaaa
     4261 tgaaaaagac gcttggattg ttgttgatct gcagtgcggc ggccggggtt cttcaggggt
     4321 gtgcttacgc cggcgtggcc gcgagcggtg agcgcgccgt ggtggcccgt aatgacggct
     4381 tcctgttcgg cattctgcgc aaagtctatg tttgcaagat ctcggatgcc ggcttgaccg
     4441 cgtgccagga ttctgattct ccatgagcga ttcgggcggc ggggttcccg gattggggcc
     4501 gattgtcgcc cgcgatctcg gcgcgccggc gcggggatgg atccgcgccg gcgttctgat
     4561 ggggtgtatt ctcgggctgc ttggttgcaa gagagcgcgt aacgaagcat cgcaatcgac
     4621 tccggtcgtc tcggcgacca cctcggcggc tcaggacgcc tctccgcaag tgagcgcggg
     4681 ctacggcgag ccggttggcg cgcgcgtgga agctagtgcc atgacgcccg ccttcgaggt
     4741 cgcggtggct tcgagtggag aggcggtcgg tactttcgtt tcgccgctca cggctttggt
     4801 tgcgcaaagt ctgcgagatt gtggtggctt gcgcgcggtc gcggaatcca agcccgcgtt
     4861 gagcctggcg ttcagtgtgg tggctgggcg tgcccaagcc gattcgattc aggcccccgc
     4921 tctgccggaa gccgagctcg gctgcgtgag tcgaggcctg ctcggtcaga agttcccggt
     4981 cttgcccgcg ccgcagaagt tccttcttca attccgtctt acgagttaaa cgcgaaggcc
     5041 gatgtcgttc ttatctgtcg ttcacgccgc tcactcgaac ccgcgcgcgt ggcgcaacgc
     5101 tactttgatg ctgctgagtt cgtgctgggc gacggtggca ggggctcagc ctgccgtcgc
     5161 tcccgcggcg gggaatcagc cggcgatgga aggacaacca gcgagctcca cggccccgcg
     5221 tgctgagtcg cctgcacccg tggcagcgcc ggcagcgccc gcgggtggtt cgtcggcagc
     5281 gcctgtgtcg tcggcagcgc ctgtgtcgac accctcagat gccgcgaccg gatcggcggg
     5341 atcgagtgcg gcttcggtag ccgcgtccgg gcctgcgtcg acggcgtcgg agcctttgcc
     5401 gagcttggac tccggggcag ttgcgccggg gcatttcaag cccgcatata cgacctggga
     5461 tgccaacgtc gaaggtggct acgggcgcta tttccctgac aagagcggac tcggtttcgt
     5521 gcgtgggcgt gcaggcgttc tgttcgccca cgaccctgcg tattacgcac tgggtgtgac
     5581 gtacgaatgg tcgaatcaag ctccctgggc tttcggcgtg caagcggaga tgctgcgtat
     5641 cgagactggc ctctgggcac aagccggcgt tctgatcgat tccaatatca aagcgggcgc
     5701 gacgttggca ttggggtgga gcatcttcgg cgtggagggc caagtccgat cgttcgatcc
     5761 ggagccctat gggctggccg tgctcggcaa agtgcgaatc ccgatctcgt tcatcgccat
     5821 ggctttcaag tgactcgagc cttgagcgac ccgtgactct gggctggtac tgagcacacg
     5881 ctcagctgtt cagcagaccc ggcctgtcgt ggatcatacg tgagaggcgg tcgcctcttg
     5941 cgttttggcg cgtccgaatt cattctcaga cgcatgataa aaaacctcat cgtgatcagc
     6001 gccgcgtggt tttcggcctg tacgctgggg aaggcgagcc cgcccccgga cacgtatcaa
     6061 ggagtggttg agctggagga gacgcaggtc gccttcgaaa cggctgggcg cgtgcggagg
     6121 ctcaccgtgg agaagggcga cctcgtccag ccagaccaac tgctggcgga gctggacgat
     6181 gagctggccc gcgtctcgcg ctccgggcga gtaatggagg cggaggcagc gcgctcgcaa
     6241 tcggccctgg ttcaagcgcg agccaaagcc gaagacattg cggcgctcgc cgcgagagta
     6301 cgcgcagctc aggccgccga ggcgctgtta acgaagaatt tggcgcgtca aaaggcattg
     6361 ttcgagtcct ccgccgtgag cgcggcgagc ttggatgagc tgaccgggca actcgatcgg
     6421 gcccgcgccg accgagaagc tgctcaggcc caactggcgg cgctcgagcg cggggctcgg
     6481 cgagaggagc gggacgttgc gttggcgcga gcggaagcgg ccgaggccgc gctgagtctg
     6541 gaggacgaac gcatcgcgcg catgaccctg aaggcaccac gaaagggacg cgtgctcgac
     6601 cgtttcgtag aaatcgggga agtggtggcg gggggcgccc ccgtctacac gttgggtgat
     6661 accgagcggc catacgtgga tgtgttcgtg ccgcaaggtc agctgagcgg tattcaggcg
     6721 ggctcgcggg cggagctcca cgtggactcg gagacgcgct cgttttccgg cgtggtggag
     6781 cacgtctcgc gccgcaccga gtttacgccc cgctatttgt tcagcgagca ggaacgcccg
     6841 cacttggtcg tacgcgtgcg actcagattc caagatcccg gagagcgtct gcacgcaggc
     6901 gtacccgcgt tcgtgcacat cgcgagggac cagccatgac cgaaggagcc attccggacg
     6961 agtcgagtct cgtgattgaa acggagcgac tcggccgtcg cttcggttcc ctggtggcgg
     7021 tgcgcgacgt gtcgctcgcg gtacgtaagg gggaaattta cggtgttctg ggacccaacg
     7081 gcgccggcaa gtcgaccacg attcgcatgc tgtgcggcat cctcgacccc agttccggcc
     7141 gtggacgggt ggtgggttat gacatcgtta aggagcccga gcgcatcaaa cagaacattg
     7201 ggtatatgac ccaacgcttc agtctgtacg aggacctgag tgtagaagaa aacctgaact
     7261 tctacgccgg gatttacggg gtaccctggt tgacccgtaa acagcgcgtc tcgagtgtga
     7321 tcgagcgggc aggcttgagc gatcggcgca agcaaattgc cggcactctg tcgggcggct
     7381 ggaagcagcg cgtggcattg gcgagcgcga ctattcatga gccgccattg ctgttcttgg
     7441 acgagccaac ggcgggggtc gatccggtta gtcgacgaga gttttgggag cagattcacc
     7501 gcatcgcggc cgaggggacg acggtgctgc tcacgaccca ctacatggat gaggcggagc
     7561 gttgccaccg tttggccttc atcttcggcg ggcgcttgtt gggggtgggc acgccggaag
     7621 agatgacgtc gcgagcaggg atcgccatgt tggagttggt ggtctccgat ccaatcgccg
     7681 ccgccgcggc gttgaggcgg cggcccgcgg tggaggaagt ctcgcactac gggcacgtca
     7741 tgcgcgtggc ggtcaacgcg agggtagaac cggaaccgca tcgactcgtg cgtgagagtt
     7801 tggcgagcgc cgggatcacg ctgcaatcga tccaagagag ccgcgtgacg gtggaggacg
     7861 cgttcgtggc cacggtgcgg cgcgaggggc aagccgcggg aggcgcatcg tgatcgcacg
     7921 gccactggtc attgcgtaca aggagctgct gcagctgcgc cgcgaccgac tcacgctcgc
     7981 gatgatggcg atgctaccct tattgcagct ggttttattt gggtacgcga tcaacacgga
     8041 tgttcggcac atccctacgg tggtctacga ctccgactac agcgcggcct cccgtgacct
     8101 ctcgcgcagc ttggaagcca ccggcttcta cgacatcgtc gggcacgtgc acggaccgac
     8161 cgaggtcgag tcggctctgc gcagtggtcg ggccaaggtc gcgctggtag tgcccgcgaa
     8221 ctatgcgcga gacttggtgc gcgggcgccg ggcaggcatc gagctgatcg tggatggctc
     8281 ggatccgcag accgtggcga gcgccagcaa tacggccgct tcgctggttg cggcgcgctc
     8341 ctccgagttg ctgctatctc gggtcaaacg cgccgggtta ccgggcttcg agcccatcgg
     8401 attggaaccg aacatctggt acaacccgga tttgcgcacc gcggtgttcg tggtgcccgg
     8461 attggtggga gtgattctga cgatgaccat ggtgatgctc acggccatgg ccatcgcgcg
     8521 cgagcgcgag cgcgggactt tggagcaatt gatcgtctcg cccgtaaagc gcgtagagct
     8581 cgtgttgggc aaaatcgtgc cgtacattct gatcggttat ctgcaaatgt cgttgatttt
     8641 gttgctcggt cgcctgctgt ttcaggtgcc gctcgcaggg tctctcgcgc tcttgtatgc
     8701 gctctctttc ctgttcattt cggccaatct ggcgttggga ctgttcttct cgactctggc
     8761 caaaactcaa cagcaggcca tgcaaatgtc cttcttcttt ctgcttccga atatcctgtt
     8821 gagtggcttc atgtttccat tcgaggcgat gcccgcagcg gcgcggggca tttcgcaaat
     8881 actaccgctc acgcactttc tgaggatcgt caggggcatc accttgaaag gcagcacctt
     8941 cgaagatctg cgggccgagc tggtatggct cgcctgcatc ttggtcgcgc tcattacctt
     9001 ggcctccctg cgcttcacga agaagctggt ctaggaaacc gcacgcgatc atgcctagac
     9061 ccaagagcga tatcgatact cgcatcgtac acgcagcgcg cgagcggttc ctgcaagagg
     9121 gggtagacgg cgcttcgctg cgcgccatcg cgaaagacgc gggcaccagc attggcatgg
     9181 tgtactacta cttcccgacc aaagacgatt tgttcctcgc cgtagtcgag gaggtttacg
     9241 agcgcgtgct cgccgatatg gccgaggcgt tgtcaccgag tttgccggtg gcgacgcggc
     9301 tgagccggct ttatgcccgc gtggggcagc tcaccgagga ggaggagatc acggtgcggc
     9361 tggtcgtgcg agaggcgctc gtctcctccg cgcgcttgca acgcttgatc gagcgcttcc
     9421 aacgcgggca catccccttg attctacgca ccgtcgcgga cggcattgcg gatgggacgc
     9481 tggccccgaa tcgacatccg ttgttgctga tggccgccac gctcgccttc ggcgccgcgc
     9541 agcagatcct cactcaggtg ctcgggccgc gtctcggccc cgcgctcgcg ctgccgccgg
     9601 ctcaggaact gccaggacag ctcgtcgaca tgctgctgca cggtgttggc tccaagcggg
     9661 ggtgaccgcg tgagagggcc atcaaccacg tgagctgcgc gccggctcgg cgaacgccac
     9721 cggtgaggtc gactcggaag ggcgagccag cgcttccgaa gcagcagcat cgcgaatggc
     9781 cgcttcacac gcagcagagc aggccctgat cagctcttgg acggcggtgg ggattcgacg
     9841 tggatccgca gcctcggcgc gcagcatccg ctcgagctgg cgtgccgtat cggtgatgtc
     9901 ttggaatccg aaggtgccgc ccgagccggc caagcgatgg gagagggcca acaactgttc
     9961 ccgatcggag ttctcggaag cttgctgcaa atccgtgatg cgatgacgca agtcctcgat
    10021 gaactcgagt acgaactcgc gcagctcggg gcgctgagac agcaggctgc gcttgggtcg
    10081 gatttctccg cgggcgcagg gcatcgggcc agactgctgc agttctttgt aggcgagcgt
    10141 cgcttgcgag gggtagccgt gcaggtgaaa atcggccaag caccggacga ccgcggtcgc
    10201 gtcaatcggc ttgcagacga cccagttgaa accagcatcg agatattgct cgcggccgct
    10261 ctcgctcacg tcgccggtga gcgcgatgat tgggcgcgtg aagccgccgg ttcgtagggc
    10321 cgagaccgca tcacagccgt ccaggcccgg cattcgaacg tccatcagca ccagatcgta
    10381 gtgagtgcgt aacgcggcga gtaccgcatc ggcgccgtcg tccgcgcagt gcacttctgc
    10441 gcccgcccgc ccgagcacgg cactgagtag ggagcggatc tctggcgagt cgtcgaccac
    10501 gaggactttg acgccggcca aggacgtcgc cccctcgcgg ccttcgcccg ccgcactttg
    10561 ccgagcaacc tggttcgaag agacttgcag ggggacgcgc agcgtgaaag tgctgcccac
    10621 gttcagctga ctctggaccg tgatgtcacc gcccatcagt cgcgccaagc gttgcgagat
    10681 ggcgaggccc agcccggtcc cttctcgtgt accgttcgac gcgttttcca cgcgcttgaa
    10741 gggtacgaac agctgagcca ccgcttcttg actcatgcca attccagagt cgatgatttc
    10801 gcacacgagc agggcttggg gcgcgtcccc ttcgaggcgg ctctggacgc gtacgccgcc
    10861 gtggtccgtg aacttcagag cgttcgctac cagattgacc aggatctgcc gcaagcgacc
    10921 ggggtctccc acgatgatcg tggacgcggt cggctccacg tggagatcaa tccctcgcga
    10981 ggtggcctgg acttgcacga ggtcacgcac gtcgctgagc acttccccga gctggaactc
    11041 ccgactctcg agctcgaggc cgctcttttc gagagactcg aagatgagca gctggttgac
    11101 gatctcgagc agctgccgcc ccccgcgcga gatcacccgc agatcctccc ccgcgctcgg
    11161 cgggagctct ttctcgagca actccgcgta gccgatgatc gacgtgagcg gcgtgcgcat
    11221 ttcatggctg acgtaggcca tgaattcact cttgcggcga tcggcggcct ccgcggccga
    11281 gcgcgccagc tcgagcgcgt tggcgtgagc tcgcagctcg acattttggt gcacggccgc
    11341 ccagaagcta cggtgttggt gtgccgcctg cagccatgaa tacaagagaa acaagcaaat
    11401 ggagccagac acgcccgcac catgcaggcc ccagcgtagc gccacgctcg aaatcaccgg
    11461 caccagcatg agcagcagat aagcgcggac gagccgtaaa tcgggagaca agctcgaggt
    11521 cgcgcccgcg acgatcccgg cggtgggaac gagcagggtg agcgtgtctg cgtcgagttg
    11581 ctggcctgcc gcggctgcgg cgctcgatcc ccaaatgccc gcgctgaaga gcgcgccgat
    11641 ccggaagcgt cgaagccact gacgggcgtg gtgttcactt tcgccctcgc tgccctgcat
    11701 agtcaggcgc agcaccgcgc agcccaggag cagcaccagc gcggacgtgg tcgcccaagg
    11761 gtgatgcagg cgaaagcggg tggtggccgc taccgctagt gccagaaacg cgacgatgat
    11821 tgcgcccatc tgcgagcgct cgcgtaaggt tcgtgccgtg actcggacgt ccgattccgt
    11881 ttcgcgacgc agcactgcgg tgctcacgga ggcttcggag ccctcatgtg atccggagtg
    11941 cgcgcgtacg aggctcgtca gttcgcgaat gatcgtccct cagcagtgga aacagtagtt
    12001 ctccgatacc gcgtacggcc gcccatgcgg ccccttcagg cgggttcgcc agatttctcg
    12061 ggatgcactg cctgaggcat tgctgcccgc ggcgggtgtg agcggggaac cccaatcgtt
    12121 tacgcgctca tccacaggag gtatgaagcg cgtcatgttc aagcttcagg tcattgccgt
    12181 cagcacgcgc gaagggcgag cgggactgcc tttggtgcaa tggttttcga gccgggctat
    12241 ggcgcacggg gggttcgacg tggagctcgt ggatctcaaa gacgtcgcgc tgccgatgtt
    12301 cgacgagccc gcgcatccac gcctcaagaa gtatcgcggt gagcacacga aggcctggag
    12361 tgctcgcgtg gacgcggcgg acgccttcgt gttcgtgatg cccgagtaca atcatggggc
    12421 gccgccgtcg ctgatcaacg cgctcgatta cctatcctcc gagtgggctt acaaggcgtg
    12481 cgggttcgtg agctacggcg gcctctcggg cggcacacgc ggcgtagtgc acgccaagca
    12541 ggtgctgagt gcgatgaaga tggtgccgct cgtagagtcg gtggcatttc cgttcttcaa
    12601 caagctgttt agagaggatg gctcgttcga cggtagccag caggaggctg cggcgaaaac
    12661 catgctcgac gagctggtgc gttggacgca agcgctgacc accttgcgtg ccccccgcgg
    12721 ctaactgcgc cgtcgagcgc agtaaggctg ccgatcaggg gggcatgccg cagcggatcc
    12781 aagtcaagac gagctggctc tcctgcgcct cgatcgatac ctcggagtct ggcggaggca
    12841 tcgagcattc gaggagcgcc gtgcgaacgg tgtcacgcca ggtggctacg tgtccgtagt
    12901 ccgtgagtgc ccaggggccg cccttggttc cggagtgaca tacgacgcag ctgcgctgga
    12961 agatgggctc gatgtcggct cgataggagg gggcgggctc tggacagctc gagggcgctt
    13021 gcgcggcgca gagaacgagc gaaggcgttc taccgccgct gcttggttgg cggtctcctc
    13081 cgtgtccgct ctgtccgccg ctcgcggctc caaccgactc cggttctgag ccacaggcac
    13141 cgagaagagc gagccacacg atcacgcaag cttggatcgt gtggcttggg gcgcgcgccg
    13201 ggcgggtgga gaaaggaaaa ttctcctcgg accgattggc tggcacccgt cgaggttagc
    13261 tgcgcgtact cgtaatgcca atacgaagcg tgatctggac cacaactcga gcacgcaagt
    13321 accaatccgg cagtaccaat ccgggctggg taccgtgcgc gcctgcgaca aattaccgca
    13381 ttgcctctga acttctttgc gggcaattcc accaacgtga tgagattgac tccgatgctt
    13441 tgcaagtcga ccgctttgag gattggattg gtcttgattg ggccaatggt gagcggggcg
    13501 gcgcatgcgc acgtgtcgat cgctagtggt cc
//
LOCUS       DASTKU010000114         4140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_468580, whole genome shotgun sequence.
ACCESSION   DASTKU010000114 DASTKU010000000
VERSION     DASTKU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468580"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VFQ61_04035"
     CDS             complement(<1..782)
                     /locus_tag="VFQ61_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953645.1"
                     /translation="MSKNTGSLRYLGYGGWALTAALMSCSKAEHVNGSDTPGPAVSSC
                     GALDLAQGVQLLSARLEHFATAGLASLAGLENSRAAARVLSLGSEHVLEPFVAETQAD
                     LRESISELRDEQLVSGNVESSTGSSITFLIHPETACRSEIAPTAPPVSGSGGAFAGTG
                     GTTNGEKPIGDTPELDPDCVKRFTEHPVRLRLSRIACDQGENLALEVFVNADRERLLL
                     AELYSERAELELNAGLFFREVMSVEYEDLPDGTGHNVEKPVFT"
     gene            1191..3053
                     /locus_tag="VFQ61_04040"
     CDS             1191..3053
                     /locus_tag="VFQ61_04040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET9953646.1"
                     /translation="MAHRKDIDGLRAIAVLSVLIFHLGWGQPFSGGFVGVDIFFVISG
                     YLITGIVQADIEASRFSIAEFYERRVRRIFPALFVVYAATLLAASFVLFPGEAKHVGH
                     DVLWSLGFLSNVAFSRAAGYFDQSAHNAPLLHTWSLSVEEQFYIGFPLLLWLLGKTTG
                     WVRWAVLGLLTVSSFALSEWLLRFSARDAFYLVHCRAWELLLGALLALAARRGSIVHR
                     HRWSAELIGVFGLGLIAFAIVAIQEDTAFPGVWALPPCLGAVALIYSGGVRTTLASSL
                     LGSAPLAFIGRISYSLYLWHWPMIALYTARHQELTNPARWIILGASLGLATLSFYFVE
                     QPFRRKPYRLSTRLTLASAAAGMLGVAGLALGVPRAAAQLRPASQQQEQALSYLDYSF
                     DVKGGTCFLSGGFDDVKLFREDLCLALRDDRKNYLILGDSHAAHFAPAFRALRPDINF
                     LQATASGCQAVRDGGGATRCTRLFRKIFDEFLPNHHVDAVVLAGHWPKHNLRALLRTI
                     GYLRDKADRIIVLGPVVEYRQVFPLLLARSIAENNPDLIEQNRAPYERQLDRFYASRV
                     KRPAEYYSVYSATCPGDECTLWAKPGVPMQYDRHHLTFEGAKLVLERLGPKLFR"
     gene            complement(3184..3615)
                     /locus_tag="VFQ61_04045"
     CDS             complement(3184..3615)
                     /locus_tag="VFQ61_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953647.1"
                     /translation="MLKRIPVLVGGLMVSAAMFSGSAQAADQEGLVNVNIEDVTVQIP
                     IAAAANICDLNVLVLAQQLLTGGGVCKAGAITLAQDNDGSGGGATQDGLINVNLSNVD
                     AQVPITIAANICGVAVDVLSTALGSGPVDCNAYGKNAAVSR"
BASE COUNT          737 a         1320 c         1205 g          878 t
ORIGIN      
        1 gtaaacaccg gcttctcgac gttgtggccc gtgccatccg gcaagtcctc atactcgaca
       61 ctcatcactt ctcggaaaaa cagacccgcg ttcagctcca gctccgcgcg ctcggagtaa
      121 agctcggcga gcagcagccg ctcgcggtcc gcattcacga acacctccag cgccagattc
      181 tctccctggt cgcaggcaat ccgtgaaagg cgcagccgaa ccgggtgctc cgtaaatctc
      241 ttgacgcagt cagggtcgag ctccggcgtg tctccgattg gcttctcacc gttcgtcgtg
      301 cccccggtcc ccgcgaaggc ccccccagag ccactcacgg gtggagcggt gggggctatt
      361 tcgctccgac aagccgtttc tgggtggatc aagaacgtaa tgctgctgcc ggtgctcgac
      421 tccacgttgc cgctgaccag ctgctcgtca cgcaactcgg agatgctctc gcgcaaatcc
      481 gcctgtgttt cagccacgaa cggctcgagg acgtgctcac ttcccaaaga caatacgcgg
      541 gcagccgcgc gtgaattctc gagcccagcc agcgaagcca agcccgcggt cgcgaagtgc
      601 tccaaccgcg cgctgaggag ctgaacgcct tgcgccaagt ccagcgcccc gcagctgctc
      661 accgccggcc cgggggtgtc actcccattc acatgctccg ccttcgagca tgacatcaac
      721 gccgcggtca acgcccagcc gccgtacccc aggtaacgca aacttcccgt attcttgctc
      781 attccaagtg ctcccaaccg ccaaacccta gttaactagc gaaatccaag cagagatcgt
      841 gccagcagct gcaggccacg ctgccgacgg gcgcgactcg ccaaaccctg cacgcgcaag
      901 ctctgtctct tctcgtcttc gccccccaaa caccgcgccg gccccccttg taaagttccg
      961 gattcacgtg tgcccagcgc gccacgtctt ggagcgtcgg tctcagagca ctcgtatgga
     1021 gcatctaacg tggcgtcacc caggagagcg attgctctcc gcgtcgcgaa gacgccacgc
     1081 tcgaagactt caggtccgcg gactgtaggc cgaatggctc aagatccagc cgacgcgggt
     1141 actcctggac tagctcgtct ccggatatgc tctaagctcc cgccgctttc atggcccaca
     1201 gaaaggatat cgacggacta cgtgccattg ccgtgctctc tgtactcata tttcatctgg
     1261 gttgggggca gccgtttagc ggcgggttcg tcggggtaga catcttcttc gtcatttccg
     1321 ggtacctaat caccgggatt gtgcaggccg atatcgaggc ctcacgcttc agcatcgccg
     1381 agttctacga acgccgcgtc cggcgcattt ttcccgccct gttcgtggtc tatgccgcaa
     1441 ctttgctggc cgcgtctttc gtcttgtttc cgggcgaggc caagcacgtg gggcatgatg
     1501 tgctttggtc gttggggttt ctctccaacg tcgcgttctc gcgtgccgcc ggctacttcg
     1561 atcagtccgc gcacaacgca ccgttgctgc acacttggtc tctctccgtc gaggaacagt
     1621 tctacatcgg ctttccgctg ctgctctggc tgctcggtaa aaccacgggt tgggtgcgtt
     1681 gggcggtgct cggcttgctc acggtctcga gcttcgcctt gagcgaatgg ttactacgtt
     1741 tctcggcccg agatgcgttc tacctggtgc attgccgtgc ttgggagttg ctgctcggag
     1801 cgctcctcgc gctggccgcg cggcgaggtt caatcgtgca ccgtcaccgc tggagcgccg
     1861 agctgattgg agtgtttggg ctggggctca tcgcgttcgc aatcgtcgcg attcaggagg
     1921 atacggcttt tcccggagtt tgggcgctac ccccgtgtct gggcgcggtg gcgctgatct
     1981 attccggggg cgtgcggaca acgctcgctt cgagtctatt gggcagcgcg cccctcgcgt
     2041 tcatcggccg tatttcgtac tcactctacc tctggcactg gccgatgatc gcgctctata
     2101 ccgcgcggca ccaagagctc accaatccag cgcgctggat cattctaggt gcgagcttgg
     2161 ggctcgcgac gttgtcattt tacttcgtcg agcaaccgtt tcgtcgtaag ccgtatcggc
     2221 tcagcactcg actcacgctg gcctcggccg ctgcgggcat gcttggggtc gctggattgg
     2281 ccctcggcgt tccgcgcgcc gctgcacagc tgcgacccgc ttctcagcag caggaacagg
     2341 ctctgtcata cctggactac agcttcgacg tgaagggcgg cacctgcttt ctgagcggtg
     2401 gcttcgacga cgtgaagctg tttcgtgagg atctttgctt ggcgttgcgc gatgaccgca
     2461 agaactatct aatcctgggg gatagtcacg cagcgcactt cgcgcccgcg tttcgtgctc
     2521 tgcgtccgga catcaatttc ctgcaggcca cggcttcggg atgccaggcc gtacgcgacg
     2581 ggggaggtgc gacgcgttgc actcggctat ttcgaaagat cttcgacgag tttctaccca
     2641 atcatcacgt cgacgcggtc gttctggccg ggcattggcc aaaacacaat cttcgggccc
     2701 tgctccggac cataggatac ctgcgcgaca aagcggatcg catcatcgtt ttgggccctg
     2761 tggtggagta ccggcaagta ttccctttgt tgctggctcg atccatcgcc gagaacaatc
     2821 ccgatttgat cgagcagaac cgcgcgccgt acgagcggca actcgaccgc ttctacgcct
     2881 cccgggtgaa gcggccggcg gagtactatt ccgtgtactc ggcgacgtgc ccgggcgatg
     2941 agtgtacctt gtgggccaag cccggcgtac ccatgcagta cgatcgtcat catctgacgt
     3001 tcgagggggc aaagctggta ttggaacgtt tgggccccaa gctgtttcga tagaggccgc
     3061 tctcgacggc cggccttcgc aatcgactcg gcagaaccct atttctagag gcccgtgttt
     3121 tcacaggcaa ggttctgccg agccgagatg cgtcgtcagg cgccaaggat tcgccggtcg
     3181 ggctcaacga gagacggcgg cgttcttgcc gtaagcgttg cagtccactg gaccgctgcc
     3241 gagtgcggtg gagagcacat cgacggccac gccgcagatg ttcgcggcaa tcgtgattgg
     3301 cacttgcgcg tcgacgttcg ataggttcac gttgatgaga ccgtcctggg tggcgcctcc
     3361 gcccgacccg tcattgtctt gcgctagcgt gatcgcgccc gccttgcaca cgccaccgcc
     3421 ggttaggagt tgctgcgcga gcacgagcac attcagatcg cagatgttgg ccgcggccgc
     3481 gatcgggatt tggaccgtga catcctcgat attcacgttc accaagccct cttgatctgc
     3541 cgcctgcgca ctgccggaga acatcgctgc agacaccatc aagccaccca ccaggacagg
     3601 aattcgcttc aacatgactt cacctattca gcaagttgcg tttcgtttaa aaccgctgct
     3661 tcgttgcagc gcgacagact gcgagtgcac tctgcatgcc atcgctcggc gaacggaacg
     3721 ccagacatcg attttgccga gtacacgagc gcttgcgtag gtctcgagtt cgaaagcgca
     3781 tcgacccaac cgaattgcgc gtctcagtga cgccacgacc gcacactccc taggtacagg
     3841 agtggttcga atgcgaacga aaatcagtgt cagcgtcgcg ggaacccaag cgtgcgacca
     3901 agttgcatcg cagatcgttt gtaggaacta cgattccgag aactgcgtca tacgggcact
     3961 gcgggccatt gcggagcgac gccgatggat gtcgccgctc aactagtgca ccgcatgtga
     4021 cttgagttca atttttggag gggttgcctc gtcgaacgat gcgtggaacg aagcaacgac
     4081 ttgaagctaa gcgccgcgga cagaaagacc ctgcagaagt taatcgcgcg tccgtccgcg
//
LOCUS       DASTKU010000115         6503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1136667, whole genome shotgun sequence.
ACCESSION   DASTKU010000115 DASTKU010000000
VERSION     DASTKU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136667"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VFQ61_04050"
     CDS             <1..1207
                     /locus_tag="VFQ61_04050"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9953648.1"
                     /translation="SAQNILISREGHIKISDFGIAKALGSARDSTKTGHVRGKRSYIS
                     PEHALGLPTDRRSDVFSLGVVLYLATAGRFPFREDGCATEAPMARLLSARCLPPSRVC
                     SGYPLELEWIVMRALARDPAARFPSADAMRSALEQYMLRSGAFVTDRDVARVLIERRG
                     AEIAKREQRVRLLSAASEAATSCAGFSQTLDPVLPCSRTLSPPLSWRAAGRQALLVLA
                     VSALGTAVGIGTKARTGSGAHSAALLGRAGLHMRVGSAASMAATGSQTAARAASDGDA
                     AALGASASPAQASTEPSAIQIWLPGKSRSKTTEESSVGAALPARLGASPSTHDSRALR
                     AHRTERVFSVQPSSGASPDNMNLGDVRPGVAAAPGMPPPAAAAAPRKVAPSQADPPRV
                     PIGPLEQEL"
     gene            1324..2280
                     /locus_tag="VFQ61_04055"
     CDS             1324..2280
                     /locus_tag="VFQ61_04055"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9953649.1"
                     /translation="MLARTLFSEGNQLVEQGAYAEALQKYESAYAAWGNPKIHLNLAT
                     TLRALGRHAEAANTYVRYLLEAQPSPERRNEVIGIVEALATQLGRLRLPRDPSVQRVA
                     VDGRQLVLDRLDFYFVEPGSHDLILETAPPGQAQTVEHQCLELEPGEERAVRRQVLPH
                     SELRPRLQRSPLTDAPREASRNLNPMAWDHTGSEWFALARVDIDAAGRGGVGAVGMSR
                     ALGAHARIQGGALLGARGGAWVGMEVVATTQPVRPSAGFSLPTFFVSGVRAGLAGDVT
                     LRLSVDSRWQPFVRASAAYFPSVPPNFVAFVLLPSLGLEVKL"
     gene            2277..3743
                     /locus_tag="VFQ61_04060"
     CDS             2277..3743
                     /locus_tag="VFQ61_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953650.1"
                     /translation="MSRRTGLVLALLSSCTHPGGSEPGEVERRALPLEVVHFVPVANS
                     PEANARVHHGFVYDPSRRVSIAFGGHPPGDREGSLPDTWTWDGQAWREVDGYPYRAFV
                     SGAFQLQESKLLTFGGFDHTQASGYSRETWEFDGELWSKRQTAVQPSQRSAYGLAYDS
                     ERKVVVLFGGYYGEFKSDIWEWDGSTWTERCTRRPCSTAPRPAARGNAMLVYDAARKV
                     SVIYGGLDWERGYSDTWVWDGTSFTEIKTTENPDVRESVAATYDPVSSRVLLFGGIGM
                     SGLELNDLWSWDGRTWTLIAPTTPATPRQGAALVWDEARRRATLLGGERDGQAVEAWE
                     FAFSGGSCAVDAECHSEQCQDQICAPPTGALGAGGDSSVAAEGGAAGTSEGGAQGAGA
                     PASTHTGGSPNGAGADDGPEMGTSGGAEVGWAGSPPTPATRLSARSTSFYACSVTRAQ
                     LGPTRVGLWLLLATCAVLLRRRVRPSSATRNRAPDHAN"
     gene            complement(3740..5020)
                     /locus_tag="VFQ61_04065"
     CDS             complement(3740..5020)
                     /locus_tag="VFQ61_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9953651.1"
                     /translation="MHTCVRRWKAAPMVLISAWSFGCGADASTSRRPSHSNGGAVAMQ
                     QPPPAGTQAASGGRAGPLLPPAVSRPPSAGSGGTAGSEVPNASGGAPTCGSVQIHSDV
                     KTTEMVIETPGNVLFVFDQSSSMNEPWNGTPKWSVANSALVAAFTPLQGRLSAGAVLY
                     PTPDTSMPTPTCNWAVDWVACLAGLASGNGNLCPDVKPIGSSPQIRILPGAEFLKQWS
                     SLWTTTQAATGYGTPTEKGLLQARAALEHPPVGNTAVVLVTDGEPTCGSNEANIAAEL
                     LQKGIKTYVVGLPGASGAALLDSVAIAGGTAMQGCTRNCYISPSNPAAFQSALAQIAS
                     TTITTMTTRTIENCSFQLEPAPGADPNEVHLVVTDAQNMQQYEVPRPDGWTLGADERS
                     AMLSPDLCERAKSGAFLDLTFQYGCVTAPVLPPR"
     gene            complement(5128..6318)
                     /locus_tag="VFQ61_04070"
     CDS             complement(5128..6318)
                     /locus_tag="VFQ61_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953652.1"
                     /translation="MLQSSSAAGAPVGWAALLISVGCSREPHLDVETPTGGATQECPL
                     VSAGPCAGRAASGGSTASSGFSGGGALGGGASDTTRGGAQPSGGATESGTSVTHGGAG
                     SAGAPVGGRSTQLEGSPSAGSAGASASSSSGGASPPDALIRGDAPTPQSTLAPGPYAV
                     ATLTSGLRDGPAYGSQTLHYPTDTGAPFAGVVVVPGFVSPESSMRAWGPFLASHGIVT
                     LTIGTNSGGDPPEMRAKALLDGIETLKAENERSTSPIQGKLATNRFAVMGWSMGGGGT
                     LIAANGNPTLKAAIGLAAWSPGQQFTEDQVPTLLLAGSADVLAGGQSQGFYSSIPNST
                     PKMLFEVAGGDHSIANDPSSARGEIGRYGLSWLKVFLEGDLRYREFLRVKPTQSSDFR
                     ANIE"
BASE COUNT         1081 a         2109 c         2141 g         1172 t
ORIGIN      
        1 ctcagcacaa aatatcctta tatctcgcga agggcacatc aaaatatcgg acttcgggat
       61 cgccaaagcc ttgggcagtg cgcgggacag caccaagacg gggcacgtgc gaggcaagcg
      121 ttcttacata tcgccggagc atgcacttgg gctgcccact gatcgacgga gcgacgtctt
      181 ctcgctgggc gttgtcctct acctcgcgac agcaggtcga ttcccatttc gcgaagatgg
      241 atgcgcgacc gaagctccga tggcgcgctt actctccgca agatgcttgc cgccgagccg
      301 tgtatgctcc ggatatccgc tcgagctcga gtggatcgtc atgcgagccc tcgcgcgcga
      361 tccggctgcc cggttcccat cggcggacgc catgcgttcg gcgctggagc aatacatgct
      421 ccggagtggc gcgttcgtta ccgatcggga cgtggcgcgc gtgttgatcg agcgacgcgg
      481 ggcggagatt gcaaagcgcg agcagcgcgt gcgccttctc tccgcggcgt ccgaagctgc
      541 cacctcctgc gctgggttct cgcagacgct cgatccagtc ctgccgtgca gccgcacgct
      601 gagccctccc ctttcgtggc gggccgcagg gcgtcaagcg ctcctcgtgc tcgctgtctc
      661 cgctctcggt acggccgtag gcattggaac caaggcacgg accggcagcg gggcgcactc
      721 ggcagccctt cttggccgag cagggctgca catgcgcgtc gggtcggctg cgagcatggc
      781 ggcgacgggg tcacagacag ctgcgcgcgc agcgtccgat ggggacgcag cagcgctcgg
      841 cgcctccgct tccccggcgc aagcgagcac ggaaccgtca gcaattcaaa tttggctgcc
      901 tggcaagagc cgctccaaaa ccacggaaga atcgagtgtt ggggcggcgc ttcccgcgcg
      961 tctgggcgcg tctccgtcaa cacacgattc acgcgcactg agagcacacc ggacggagcg
     1021 ggtgtttagc gtgcagccga gttccggtgc ctcgcccgac aacatgaacc ttggtgacgt
     1081 tcgcccgggt gttgctgcgg cgcccggcat gcctcccccg gctgcggcgg ctgcgccgcg
     1141 gaaagttgca ccctcccaag ccgacccacc acgggtcccg attgggccgc tcgagcagga
     1201 gctgtgatgt ttcggcttcc gtcgcgcaga gcggggtggt tgaggacgtg tctagcacaa
     1261 gctgccgtcg tcctcccctg ctccctatcc ttcgctgcgg agccccagcc gagcggagcg
     1321 gaggtgctcg cgcgcacgtt gttctccgag ggcaatcagc tcgtcgaaca gggtgcctat
     1381 gccgaggcct tgcagaaata cgagagcgcc tacgctgctt ggggcaaccc aaagatccac
     1441 ctgaacctcg cgacgacgct gcgtgcgttg gggcggcacg ccgaggctgc caacacctac
     1501 gtgcgatacc tgctcgaagc ccaaccttct cccgagcggc gcaacgaggt gattggcatc
     1561 gtggaggcgc tcgcgacgca actcgggcgc ctgcgcttgc cgcgcgatcc atcggtgcag
     1621 cgggttgcag tcgatggccg gcagttggtg ctcgacagat tggacttcta cttcgtggag
     1681 ccgggctcgc atgacctgat cttggagaca gcgccacccg gacaggcgca gaccgtcgag
     1741 catcagtgtt tggagctcga accgggtgaa gagcgggccg tgcgacgcca agtgctgcct
     1801 cacagcgagt tgcgtccgcg cctgcagcgc tcgcctctca cggacgcccc gcgggaagcc
     1861 tcccgaaatc tgaacccgat ggcttgggac cacaccggct ccgagtggtt cgccctggct
     1921 cgcgtcgata tcgacgctgc aggccgaggc ggcgtgggcg ccgtgggcat gagtcgcgcg
     1981 ctgggcgcgc atgcgcgcat ccaggggggc gcgctgctag gcgcgcgcgg cggtgcgtgg
     2041 gtcggcatgg aggtggtcgc cacgactcag cccgttcgtc cttcggcggg gttttcgctg
     2101 ccgacgttct tcgtgagtgg cgtgcgcgcc gggctcgccg gggacgtcac actgcgcttg
     2161 tccgtcgact cgcgctggca acccttcgta cgcgcctcgg cggcgtactt tccgagcgtg
     2221 cctccgaact tcgttgcatt cgtgttgctc ccgtcgctcg ggctggaggt gaagttgtga
     2281 gtcgacgtac agggctcgtg ctcgcgttgc tctcttcatg cacgcaccct ggcggttcgg
     2341 agcccggcga ggtggagcgc cgtgcgcttc cactcgaggt ggtgcacttc gtgcccgtcg
     2401 caaactcgcc ggaagcgaac gcacgcgttc accatggctt cgtctacgac ccgagtcgaa
     2461 gagtcagcat cgcgttcggt ggccacccgc cgggggatcg tgaaggctcg cttccggata
     2521 cttggacttg ggacgggcag gcttggcgtg aggtcgatgg ctatccctat cgcgcgttcg
     2581 tgagcggcgc attccagctg caggaatcca agctgttaac gttcggaggc ttcgatcaca
     2641 cccaagcctc cggttacagc cgtgaaacgt gggagtttga cggcgagctc tggagcaaac
     2701 ggcaaactgc agtgcaaccg tctcagcgca gcgcctatgg cctcgcctac gattccgagc
     2761 ggaaggtcgt agtcctgttc ggtggctact atggtgaatt caagagtgat atctgggaat
     2821 gggacggttc cacgtggacc gagcgttgca cccggcggcc ctgctccacg gcgcccaggc
     2881 ctgccgcacg cggcaatgcc atgctcgtct acgacgctgc gcgaaaggtc agcgtaattt
     2941 acggaggcct ggattgggag cgtggctatt cggatacttg ggtttgggac ggaacgagct
     3001 tcaccgagat caagaccaca gagaacccgg atgttcggga atccgtggcc gcaacctacg
     3061 acccggtgag ctcgcgcgtg ctcctgttcg gcggcatcgg catgagcgga ctcgagctca
     3121 atgacttgtg gtcgtgggac ggacgcacct ggacgctgat tgcgccgacc acacccgcga
     3181 ctccgcgcca aggggccgcc ctggtgtggg atgaagcgag gcggcgcgca acgctgcttg
     3241 gaggcgagcg cgacgggcag gcggtggaag cttgggagtt tgcgttctcg ggcggatcgt
     3301 gcgcggtcga cgcagaatgc cattccgagc agtgtcagga ccaaatttgc gcgccgccca
     3361 ctggcgctct cggagcgggc ggggactcga gcgtagccgc ggaaggcggc gcagcgggaa
     3421 ccagcgaggg tggagcacag ggtgctggag ctccggcctc gacccatacc gggggcagcc
     3481 caaacggcgc tggcgcagac gatgggcccg agatggggac gagcggagga gcggaggtcg
     3541 gatgggcagg ctcaccgcct acgccggcca ctcgccttag cgctcgctca actagctttt
     3601 atgcgtgctc agtgactcgg gcccagctcg ggccgacgcg cgtcgggctc tggctgctcc
     3661 tcgcgacgtg cgccgtgctc ttgcgccgtc gagtgcggcc cagttcagcc actcggaacc
     3721 gggcccctga tcacgccaat tagcgcggcg gcagcacagg cgcggtcacg catccgtatt
     3781 ggaaggtgag atccaaaaac gcgccagatt tggcgcgctc acacaaatct ggactgagca
     3841 tcgcggatcg ctcgtcagcg ccgagcgtcc agccgtctgg gcgaggcact tcatactgct
     3901 gcatattctg cgcgtccgtc accaccaaat gcacctcatt gggatcggcg ccaggcgcgg
     3961 gctcgagctg aaaagagcaa ttctcgatcg tacgagtagt catcgtggtg atggtggtgc
     4021 tcgcgatttg cgcgagcgcg ctctggaacg ccgccgggtt actgggcgaa atgtaacagt
     4081 tgcgggtaca cccctgcatc gcggtgccgc ctgcgatggc cacgctgtcg agcagcgccg
     4141 caccgctcgc accgggcaac cccaccacgt acgtcttgat tcctttttgc aggagctctg
     4201 ctgcgatgtt tgcttcgttg ctcccgcacg tgggctcccc atccgtcacc agcacgacgg
     4261 ccgtgttgcc caccggggga tgttcgagcg ccgcgcgcgc ttggagcaaa cccttctcgg
     4321 tgggtgtccc gtagccggtt gcagcttggg tcgtcgtcca cagactcgac cactgtttca
     4381 ggaactcagc gccaggcaaa attcgaatct gaggagagct gccgataggt ttgacgtcag
     4441 ggcacaagtt tccgttgccc gaggccaatc ctgcgaggca cgcgacccag tcgacagccc
     4501 aattgcaggt gggcgtgggc atgctcgtgt ctggagtggg atacagtacg gcccccgcgc
     4561 tcaagcgccc ttgcaagggc gtaaacgccg cgaccaacgc tgaattcgct actgaccatt
     4621 tcggagttcc attccaaggc tcgttcatgc tcgatgactg atcgaaaacg aacagcacgt
     4681 tgccaggcgt ttcaatcacc atctccgtcg ttttgacatc ggagtggatt tggacgcttc
     4741 cgcacgtggg tgctccgccc gatgcgtttg gaacctcgct gccagccgtt ccgccgctgc
     4801 cggcgctggg aggtcgactc actgcagggg gcaagagcgg cccagccctg ccgccggagg
     4861 cggcttgcgt ccccgcgggc ggcggctgct gcatggccac cgcgcctccg ttagagtgag
     4921 acggacgccg gctcgtgctg gcgtccgcgc cacacccgaa gctccacgcc gagatcagga
     4981 ccatgggtgc cgccttccag cggcgaacgc acgtatgcat gattggatcg cctcctgcaa
     5041 ccgcgctgcg gcggtaaaca aatgctctcg gtcgtactct ctagttcgag tgaatggatc
     5101 gcgtcgagcc ggatgctcgc gcgtcgctca ttcgatgttg gcgcggaagt cagaggattg
     5161 cgtcggcttc acgcgcaaga attctcggta acgcaggtca ccctcgagga acaccttgag
     5221 ccaagacaag ccatagcgcc cgatctctcc gcgcgcagag ctgggatcat tggcaatcga
     5281 gtggtcaccg cccgccactt cgaacaacat tttcggtgtg gagttgggaa tgctgctata
     5341 aaaaccttgc gattgcccgc ctgcgagcac gtcagcgctc cccgcgagca gcagagtcgg
     5401 gacttggtcc tcggtaaact gctgccccgg gctccacgcc gcgagaccaa tggcagcttt
     5461 gagcgtggga tttccgttgg cggcgatgag cgtgccgccg ccgcccatcg accaccccat
     5521 caccgcgaat cggttggtcg cgagcttgcc ttggatcggg ctcgtggagc gctcgttttc
     5581 agctttcaac gtctcgattc catcgagcaa ggccttggcg cgcatctcgg gcggatcccc
     5641 tccgctgttg gtgccaatcg tgagggtcac gatgccatga gaggccagaa agggtcccca
     5701 ggcgcgcatc gaactctcgg gagagacgaa cccgggcacc accacgacgc cagcaaatgg
     5761 cgctcctgtg tcagtcggat aatggagcgt ctggctgccg taggcagggc cgtcgcgcag
     5821 accgctcgtc aacgttgcga cagcgtaggg accgggcgcg agagtggatt gtggggtggg
     5881 cgcgtcgccg cgaatcaaag catccggcgg gctcgctccg ccgctgctcg aactcgcgct
     5941 ggctccggcg cttcccgctg aagggctgcc ttcgagctga gtgctccggc caccgacagg
     6001 cgctcctgcc gagcccgcgc cgccgtgagt gacggacgtc cccgattcgg tcgctccacc
     6061 tgacggctgt gctccaccgc gcgtcgtgtc actcgctccc ccacccaagg cacctccacc
     6121 agagaaccca ctgctcgccg tggagccacc cgacgcagct cgcccggcac acggacctgc
     6181 ggagacgagt ggacactctt gcgtcgcccc tcccgtcggc gtctcgacgt cgagatgggg
     6241 ctctcgacta cagcctacgc tgatgagtaa cgctgcccaa ccaacgggag ctccggctgc
     6301 cgacgaagat tgcaacatga ttcctcacgc gatgaatgaa acccctgcgc tgcacgcacc
     6361 gcgcgtcaag catgtgcttg cacgcgggct cgatactccc gatcggggcc gcggcacacg
     6421 gccacgtctc gattgctcta tgggtcgagt gcgaggaagg ccgtcacgcg ggtgggcgtg
     6481 cggtcgcgat cttccccctg cct
//
LOCUS       DASTKU010000116         6579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_691012, whole genome shotgun sequence.
ACCESSION   DASTKU010000116 DASTKU010000000
VERSION     DASTKU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691012"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="VFQ61_04075"
     CDS             <1..1305
                     /locus_tag="VFQ61_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953653.1"
                     /translation="DELLGPAGAPKSRQEICNSLGVRDGDGTDTNLNLHAVYPVYVKT
                     AASTYETRSMYEHVEGAGAIEAGAKFVKNAVLGSIKLLLCAPPDPSAPTSTFASGFVW
                     SQSPYGSYVADSSYSYNSSSSNLGGTGVDNTITQLSTGTYSVEFPGLGTETGGNVQVT
                     AYGGSSERCKVGSWNSNGSALTAIVYCHDTSGALVDTQFTASYLRHPAGAPKGGATGG
                     YVWANEATSDSYTPDPTYQWNASGALNTIERSGVGNYWVTFPGVALNGGNAQVTAYGW
                     GSEYCNVGSWGSNVVNVTCFDTTGAPADAQFTLNFSDNNPNVTPSYHYAWANEPWSES
                     YTPSTYYQRGYLSSSCSFAGTMTMRRWSTGNYTASIPALSPTGSNVKVTAYGGSSNTC
                     KVTGWYGSGDGTDVGIACFNAAGEPVDTYFTIVYSSNQYIIC"
     gene            complement(1490..2449)
                     /locus_tag="VFQ61_04080"
     CDS             complement(1490..2449)
                     /locus_tag="VFQ61_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953654.1"
                     /translation="MRSAVLPTLLLLTLGSLSGCDIEIATWHAEYLDGSPIQEEPEKV
                     GVHTSASLLLSAGWLVPKNLVILPDDPNTVRVTVGRSVFRMSEGSVEYDPPNGFENAL
                     EVFIEPLQPGSNRIRFLADNVGEVLKIPFESVEVTSTVVRSRDSVPKEGRLTAFTNAL
                     VPVAVDVYSGAEKLRGDVTLALEQPDGFASELREGILFLDDRAHHLRLSAPLPDAHLD
                     VDVVTTENVSDILVRDIWGDLPTIAVNHTLRLQIEPLDARGEIIAGAERAPFQTELVP
                     PSVGNLLPGITSLQFMSSEVGESTIRFFWGSTERRISVSVVGE"
     gene            complement(2451..3494)
                     /locus_tag="VFQ61_04085"
     CDS             complement(2451..3494)
                     /locus_tag="VFQ61_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953655.1"
                     /translation="MLTNLRSRAGVAAISFAAVQSSACQADTRDWAISISSEYPDGTP
                     VGVGLTTSVSVRIDRFPKLNWDSPEQLEVIADDDSVTVEDVGFVRTADGSYENYEVHL
                     TPKTEGSKRLRFIAENIDDVGELRFSARSVGGVRYDWDRLLDLKGRGGVGFVDSEVNL
                     NIGFTDRGGAPVNGFAGVDLEQPDGAGSSVSQNLYNKRYSVTLGPQPHALTVRSRVRP
                     EPFQIDVVDASVIAGLRWDGVIDDYSLDGSEGVTARPTLLAPDRPAVVPVGYGFYIEV
                     RPFDAFGRIIAGDPPLDPFQLTIEGHAVTTFPEVRMPVRLAAREVGQVVLHFSWGRDT
                     LDVPIQVVPRGRR"
     gene            complement(3941..5071)
                     /locus_tag="VFQ61_04090"
     CDS             complement(3941..5071)
                     /locus_tag="VFQ61_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953656.1"
                     /translation="MGGAEKNRYWGWTAALGCVASGLIIALVARERNLHKPLGDAAQL
                     VSARAGHSERALDDSNGPSLDDVASLGYARAKARIVARQWTEGLAQKLTELRDAGPAA
                     PLSCTRFAQVSISSSSAARSKRGCVANPAIDCLESTAGFFVPLDTPTGNFCDWSLWYV
                     PKRGPLRGPRELNVFAGFVESKQLGFTIRMFLVDLTGDGVDEAVVVRGRAGSSGQSLD
                     MIMATGVSEALPFDDLRDVDSDGLLDGVVDFRAVLGSKHRSSRPYRAPVLVAHRTEAV
                     RFDWEDAVSKGERAQSCRPPLTPVAIRGVGGRVMEEETIRRSICRCVEGAREATVLRE
                     VRAACENAPCAALIPTVDELLTQLSKALRWRPADADQRDHPR"
     gene            complement(5182..5254)
                     /locus_tag="VFQ61_04095"
     tRNA            complement(5182..5254)
                     /locus_tag="VFQ61_04095"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5219..5221),aa:Arg,seq:cct)
     gene            complement(5449..6060)
                     /locus_tag="VFQ61_04100"
     CDS             complement(5449..6060)
                     /locus_tag="VFQ61_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953657.1"
                     /translation="MPYFRLPVNWALMGRVGLAWAVLAWAVLACVGCGTWWSETHRTP
                     NYKLPQRLTIYLAVNERVARTDDGGIIAAMVEALEDDLREEGYAVQVLGARLGEKPPV
                     PRIELQVTDTTPENGKLRGAGELVGIVGGSVVTAGGLAGGGMVVGASGRVVVECYVVT
                     GPNHHATFAGRLAAHSWGTTTGRDSVSAGERAGHEIASELMAD"
     gene            complement(6173..>6579)
                     /locus_tag="VFQ61_04105"
     CDS             complement(6173..>6579)
                     /locus_tag="VFQ61_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9953658.1"
                     /translation="PVAAQSQRTATRSITFAGYRVDAETPVLIATALPHMLPELFPSP
                     ERFDVARYRAPRNEHRVKGAYAPFGLGAHTCLGAGMAEVQIAVVIARLLHNLEFELTS
                     ERRPPLRQDPTLTFGPKFRVRVSERRRPLGVE"
BASE COUNT         1325 a         2159 c         1928 g         1167 t
ORIGIN      
        1 gacgagctgc tcggccccgc tggcgccccc aagagccgtc aggaaatctg caactcgctc
       61 ggcgtgcgag acggcgacgg cacggacacg aacctgaact tgcacgcggt ctatccggtg
      121 tacgtgaaga ccgccgcatc cacgtacgag acgcgctcga tgtacgaaca cgtcgagggc
      181 gccggcgcga tcgaggcagg cgcgaagttc gtcaagaacg cggttctcgg ttcgatcaag
      241 ctgctgctct gcgccccacc cgacccgagc gcgcccactt ccacgtttgc gtccgggttc
      301 gtctggtccc agtcgccgta cggctcttac gtcgcggact cgagctattc gtacaactcg
      361 tcgagcagca acctcggcgg tacgggcgtg gacaacacca tcacgcagct ctcgactggc
      421 acgtacagcg tggagttccc cggcctcggc acggagaccg gcggcaacgt gcaagtcacc
      481 gcgtacggcg gctcgtccga gcgatgcaaa gtcggcagct ggaactcgaa cggctcggcg
      541 ctcaccgcca tcgtgtattg ccacgacacg tccggtgcgc tcgtcgacac gcagttcacc
      601 gcttcgtacc tacgccatcc ggcaggggct cccaaagggg gtgcgacggg tgggtatgtc
      661 tgggcgaacg aagcgacgag cgattcgtac acgccggatc ccacttacca atggaacgcc
      721 tctggggcgc tgaacaccat cgagcgctct ggcgtcggga actactgggt tacgttcccg
      781 ggagtcgcgc tgaacggcgg caacgcgcag gtgactgcct acggctgggg cagtgaatac
      841 tgcaacgtcg gctcctgggg ctcgaacgtc gtcaacgtga cctgcttcga taccaccggc
      901 gcgccggcgg acgcgcagtt caccttgaac ttctcggaca acaacccgaa cgtgacgccc
      961 tcgtaccact acgcctgggc caacgaaccc tggtcggaga gctatacgcc gtcgacctac
     1021 taccagcgcg gctatctctc gtcgtcatgc tccttcgccg gcaccatgac catgcggcgc
     1081 tggtcgacgg gaaactatac cgccagcatc cccgcgctga gcccgacggg ctcgaacgtc
     1141 aaggtcacgg cgtacggcgg aagctcgaac acctgcaaag tgacgggttg gtacggctcc
     1201 ggcgacggaa ccgacgtcgg catcgcttgc ttcaacgccg cgggtgaacc ggtcgacacc
     1261 tacttcacga ttgtgtactc gagcaaccaa tacatcatct gctgagcgcc ctcgactcct
     1321 gcattgcgcg cgccgcccgg gccatctcga gcggcgcgcg cacgcgtctc gagcaactct
     1381 cgtgcgaata tccctatctg ccaaacttcg atttgagtga atgctactgt cgagggagcg
     1441 gaccggctgc gggagctcga ggtcgagcca gcaagtccaa tggctggcct cactccccca
     1501 cgacgctcac ggaaatacgc cgctccgtac ttccccaaaa gaagcgaatg gtgctctccc
     1561 cgacctcaga gctcatgaat tggagagagg tgatcccagg caaaaggttg ccgacgctcg
     1621 gtggcacgag ctcggtttgg aatggagctc gctcggcacc cgcgatgatc tcgccccgcg
     1681 cgtcgagcgg ctcaatctgc aaccgcagag tgtggttgac ggcaatcgtc ggcaggtcgc
     1741 cccaaatgtc tcgcaccaat atgtcgctga cgttctcggt agtcacgacg tccacgtcga
     1801 ggtgcgcgtc cggaaggggc gcggaaaggc gtaaatggtg cgctcgatca tcgaggaaga
     1861 gaatcccttc cctgagctcc gaggcgaagc cgtcgggctg ctcgagggcg agcgtcacgt
     1921 ctccgcgcag cttctcggct ccgctgtaca cgtccaccgc gacgggcacg agcgcgttcg
     1981 taaaagcggt cagccgtccc tctttcggca cgctgtctcg agaacgcacg acggtggacg
     2041 taacctctac gctttcgaag gggatcttca acacctcacc gacgttgtcg gccagaaaac
     2101 gaatccgatt gctccctggt tgcaggggct cgatgaacac ctcgagcgca ttctcgaatc
     2161 cgttgggcgg atcgtactcg acagacccct cgctcattcg gaagacacta cgaccgacgg
     2221 tcactctaac ggtgttggga tcatccggta agatgacgag attcttgggt accaaccaac
     2281 ccgcgctcag caagagcgag gccgacgtgt ggaccccaac cttttcgggc tcttcttgaa
     2341 tgggggagcc gtcaaggtac tcagcgtgcc aggtcgcgat ttctatgtcg cagcctgaca
     2401 acgagccgag cgtcaggagc agtaacgtag gaagaactgc gctgcgcacg tcaacgcctt
     2461 ccgcgaggaa cgacttggat ggggacgtcc agtgtatctc ggccccagga gaaatgcaga
     2521 accacctggc caacctctct ggcggctaac cgcacgggca tcctcacctc ggggaacgtc
     2581 gtgacggcgt ggccttcgat cgtcaactgg aacggatcga gtggcgggtc gcccgcgatg
     2641 atgcgaccga acgcatcaaa gggacgcact tcgatgtaaa agccgtagcc gaccggcacc
     2701 actgccggac gatcaggtgc cagcagcgtc gggcgggcgg tgacgccttc gctgccatcg
     2761 agcgagtagt catcgatcac accgtcccaa cgcagaccgg caatcacgga tgcgtccacc
     2821 acatcgatct ggaagggttc ggggcggacg cgggagcgaa ccgtcagagc gtgcggttgc
     2881 ggtccgagcg tgacgctata cctcttgttg tagaggttct ggctcacgga ggacccggcc
     2941 ccgtcgggct gttcgaggtc cactccagcg aagccgttca ccggcgcccc gccgcgatcc
     3001 gtgaagccaa tgttgaggtt tacttcgctg tcaacgaagc cgaccccgcc gcgccctttg
     3061 agatccagca agcggtccca atcgtagcgc acgccgccga cgcttcgcgc agagaagcgg
     3121 agttcgccga cgtcatcgat gttctcggct atgaaccgga gccgcttcga accctccgtc
     3181 tttggcgtga ggtgtacctc gtagttttcg taggaaccgt ccgcggtgcg gacgaagcca
     3241 acatcctcca ccgtgaccga gtcatcgtcg gcgataacct cgagctgttc gggtgaatcc
     3301 caattcaact tcggaaaacg atcgattcgt acggataccg aagtggtgag gcccacaccc
     3361 actggtgtac cgtcagggta ctcggaagag attgaaatgg cccagtcacg cgtgtcggcc
     3421 tgacatgctg agctttgaac cgcggcgaac gagatcgccg ccactccagc gcgagagcgc
     3481 agattagtaa gcaagtgaat accgaatttt gtccgcctcg acgagccgac gcaactgcgc
     3541 ggcgtcaaaa ggacgaagcg atgcacacgt cgacggccca tcgacctacg ctgtctcacg
     3601 ctcgaacgat cgcgggagca ctcgatttca ctcagaaagg cccgcctcgg aacgcgcccg
     3661 ccgggcatgc tttcagtcag ccatcaccaa cctcgagtag cgctccaact cacgaagagc
     3721 gcagttcaac cggtgctgct cggcgatttc aaccaccctc cgaatcgaac gagagcctca
     3781 cggcaacact cggcgcaaga aagctttgcg gattttcttt gggcgattcg tgagtcaggg
     3841 tgactgaact ggtccccacc aatcagacca aaagcaggtt gtcctggcca tcgaagccag
     3901 cgcagtcggc gaacgcgaga aagcttctaa aaggatgcgg tcacctcggg tgatcccgtt
     3961 ggtctgcatc tgccgggcgc caccgcagtg cttttgatag ctgcgtcaga agctcatcaa
     4021 cggtaggaat cagcgcagcg cagggggcgt tttcacaagc ggctcgaacc tcgcgaagaa
     4081 cagtcgcttc ccgtgcgcct tcgacacaac gacaaatact gcgtcgaatg gtttcctctt
     4141 ccatgactcg ccccccgacg ccacggatcg ctacgggtgt gaggggcggc cggcaagact
     4201 gtgcgcgctc acccttcgag acggcatcct cccaatcgaa tcgaaccgct tccgttcgat
     4261 gcgcaaccag caccggggct cgatacggac gcgacgatcg atgcttcgag cccaacacag
     4321 ctcgaaaatc caccacccca tcgagcaaac cgtccgaatc cacatcgcgt aagtcgtcga
     4381 acggcaaggc ctccgaaact cccgtagcca tgatcatatc tagagactgg cccgagctcc
     4441 ccgcccgccc gcgcactacc acggcttcat ccaccccatc ccccgtcaag tcgaccaaga
     4501 acatacggat tgtaaagccg agctgcttcg attccacgaa ccctgcgaac acgttgagtt
     4561 cgcggggacc tctcagaggc ccccgcttgg gcacgtacca aaggctccaa tcacagaagt
     4621 tgcccgtcgg agtgtccagc ggcacgaaga aacccgcggt gctttccagg cagtcgatgg
     4681 ccggattggc aacgcagccg cgcttcgagc gcgcggcgct ggaacttgag atgcttacct
     4741 gtgcgaaccg agtacagctg aggggagctg cagggcccgc atcacgcagc tcagtcagtt
     4801 tctgtgcgag cccttccgtc cattgtcgcg ccacgattcg cgccttggct ctcgcgtagc
     4861 cgagtgatgc aacgtcatcc aacgagggtc cgttggagtc atcgagtgcc ctttcgctat
     4921 ggccagctct cgcggaaacg agttgtgcgg catctccgag tggtttgtgc aggttccgct
     4981 cacgggcgac gagcgcgata atcaaaccgg acgcaacaca gccaagagca gcggtccaac
     5041 cccaatatcg attcttctcg gctccgccca ttgctctgcg gcacctacga ccacacgccg
     5101 aacccgacca tgaactaccc cgaatagatc gcgcccgcaa ggcagcccga cagctctcgg
     5161 cgcatgaacc gaaagttgct gcgcccccgg gcagagtcga actgccgacc aacggtttag
     5221 gaaaccgctg ctctatcctc tgagctacgg gggctttggc tggttttcga tcgcgtccgt
     5281 gtgggcgaac cacttggacg ctcgacggga tagcacgggc gcggttggtc cggctacatc
     5341 ttcaccctgg ccacttggcc cattggggga tgggtttgtg attactgcgc gccgcatcaa
     5401 agatcgacgc aagcgccgcg tcctgccctg tcatgcgggt aatcgggcct aatcggccat
     5461 caattcgctg gcgatctcgt gcccggcgcg ctctccggca ctgacgctgt ctcgccccgt
     5521 cgtcgttccc cagctgtggg ccgcgagccg gcctgcgaag gtggcgtggt gattcggacc
     5581 ggtgaccacg tagcattcaa cgacgacccg cccggacgcg cccacgacca ttcctccgcc
     5641 agcgagccca cccgcggtca caacactgcc cccaactatg ccaaccagtt cgcccgcgcc
     5701 gcgtagcttg ccattctcag gcgtggtgtc ggtcacctgc aattcgatgc gggggacggg
     5761 tggcttctct cccagccggg cgcccagcac ttgtaccgca tagccttcct cacgcagatc
     5821 gtcttcgagc gcctctacca tggccgcgat gatgcctccg tcgtccgttc gagccacccg
     5881 ctcattcacc gccaaataga tagtgaggcg ctgcggaagt ttatagttgg gagtccgatg
     5941 ggtttcactc caccaagtgc cgcaacccac acacgcaagc accgcccacg caagcaccgc
     6001 ccacgcaagg ccgacccgtc ccattagggc ccaattaaca ggtaaacgaa agtacggcat
     6061 tgaacgagca cttcagggtg aaaccctccc ctgccccaaa acacggagta ggtcaggatc
     6121 gtcccctagg cagcgtcgga atctatctga ttgccgagcc aaactcaaca tctcactcca
     6181 ctccgagcgg gcgccgtcgc tcgcttacac gaactcgaaa cttcgggcca aacgtcaggg
     6241 tgggatcctg acgtaagggc ggacgtcgct ccgacgtgag ctcgaactcg aggttgtgca
     6301 gcaagcgcgc gatcacgacc gcgatttgga cttccgccat acccgccccc aggcaggtgt
     6361 gagccccgag tccaaaggga gcgtaggcgc cttttactct atgctcgttg cgtggtgccc
     6421 ggtaacgagc aacatcgaag cgctccggac tcggaaatag ctccggcaac atgtgcggga
     6481 gtgccgtcgc gatgaggacc ggtgtctcgg cgtctacgcg atagccggcg aacgtgattg
     6541 aacgcgtggc cgtgcgttgg ctctgcgcgg ccaccggat
//
LOCUS       DASTKU010000117         4380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1583270, whole genome shotgun sequence.
ACCESSION   DASTKU010000117 DASTKU010000000
VERSION     DASTKU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583270"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VFQ61_04110"
     CDS             complement(<1..1015)
                     /locus_tag="VFQ61_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HET9953659.1"
                     /translation="MAGLQDRVVRLLGNSVEGRSLLVANLLRRDASEVTSYWLQLCVA
                     IGIATLGLVVGSAAVVIGAMLIAPLMTPIVGLALGFSAGSPYLVLRCAGRIGFSVIVS
                     IGGAALITVLLPFHELNAEIAARTSPTLLDLVTAAFCALAGVYASLRAGSDTATTAAG
                     TSIGISLVPPLCASGYGLGTGSASTAGGAAMLFLTNFVAIVAVGTASFLFAGFNQVDV
                     VALERDELSRKGTGAPVARLLARRLAKLFESRLGPLLRLAMPLILLAAVYLPLRRALD
                     EVAWEVRVRAAVRTSIAREPLRVVQTRVRVERHEIELVMVILGTTRDAESARERIDAL
                     LRRA"
     gene            complement(1284..2081)
                     /locus_tag="VFQ61_04115"
     CDS             complement(1284..2081)
                     /locus_tag="VFQ61_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953660.1"
                     /translation="MPQNSPRSVHGCLGENANPRPVYPIAPRRTTIGRSPARLDRPRS
                     SRPIVARGALWLGTLASLLLVACSASTQKPALDTALDNPQLRHESLEATLRVTDEHPE
                     YVDELFALTLKHPKTLDRFLQNTAAGLEQDELSRQTARRLVERPAGLKQVLIATLDAA
                     SDKPASLGAAAEAMQERPQLAAMVVTQREETLRAVMRELMKEVSKNRRARLAFLASVQ
                     ENSAAMAAILAPNPEVLASLLKAFGKLGLSHGKEELEALIKAVQPQE"
     gene            complement(2193..3479)
                     /locus_tag="VFQ61_04120"
     CDS             complement(2193..3479)
                     /locus_tag="VFQ61_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953661.1"
                     /translation="MRSKHLFTLGASVLILANCGNSDPGGSGPGVSPNGGSSNPGVVP
                     ASGGMASSFGGQFTGGAFAGGQGTNVGGSGGLSSTTNAAGGVISAGAPSSGGSNNGTS
                     GSAGAASGGRGEPGGNAGRANSAGNAGRGSEGGSAGSGGTPAGASGAAGSANSGTCVA
                     SKSVNTNASGSGPHKVVVETNSDAGINEGTIFRPADLGGAEKYPIFVWGEGGCSRNGL
                     SNSAAMAEIASHGYFVIADGTPNASNSRSMDGANPAAMGKPLLGYVTWAIAENDKACS
                     AYYQSLDTTKVAANGFSCGGLMAEGTAGDPRITTWGINSSGLFGANQSFYKTIHTPVL
                     IVLGGPDDIAYENGLRDFDNISALGIPTLLFSKALGHGGDLFSRNGGDFTKIDLAWLN
                     WHLKGDEGATGKGLLVGSGCPYCSDSAWEMKSKNIQ"
     gene            complement(3663..>4380)
                     /locus_tag="VFQ61_04125"
     CDS             complement(3663..>4380)
                     /locus_tag="VFQ61_04125"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET9953662.1"
                     /translation="RSFVLHVPESYDGTTPAPLIVDFHGMGESGMSELRSSPYPAVTD
                     REGVVMAFPDGLKGPLGTAWNLGPCCVADVDDLAFARALVARVQQSACIDPRRVYAVG
                     VLTGGGMVYQLACHAADLFAAVAPAAFDFLEENVDECIPRRPITEISFRGTADPRVPY
                     GGGPSSLVPGMPITFLAFERWADIHHCSDSAPTVDDGGCSTYSRCDGDAEVALCTKSG
                     GRDDPGDAEIAWPVLKRHAL"
BASE COUNT          843 a         1435 c         1310 g          792 t
ORIGIN      
        1 acgcgcgtcg tagcaaggca tcgattctct cgcgcgcact ctcggcgtcg cgggtcgtgc
       61 ccaggattac cattaccaac tctatctcgt gccgttccac tcgaacccga gtctgaacga
      121 cccgcagcgg ctcccgcgca atcgacgttc ggaccgccgc gcggacgcgc acctcccaag
      181 ccacttcgtc gagtgctcgt cgtagtggta agtatacggc agccagcaag atcagcggca
      241 tggcgagacg cagcagcggg cccaagcgcg actcgaatag cttcgcgagt cggcgcgcga
      301 gcagtcgggc aaccggggct ccggtacctt tacgactcag ctcgtcgcgc tccagcgcaa
      361 ccacatccac ctggttgaat ccggcgaata ggaaggaagc ggtgcccacc gcgacgatgg
      421 cgacgaagtt ggtcagaaac aacatcgccg ctccgcccgc cgtcgacgct gaacccgtac
      481 ccaagccgta gccactcgca caaagcggcg gaaccagcga gatgccgatc gaagttccgg
      541 cggccgtcgt cgccgtgtcg gagcccgctc tgagtgacgc atatacgccg gccaacgcgc
      601 agaaggccgc cgtaaccagg tccaacagcg tgggagacgt gcgcgcggcg atttccgcgt
      661 tgagttcgtg aaagggcagc agaaccgtga tcagcgcggc gcctccgatc gagacgatga
      721 cactgaatcc gatccggccc gcacagcgca gcacaaggta gggcgaccct gcggaaaagc
      781 ccaaggccaa gccgacgatg ggcgtcatca atggcgcaat gagcatggct ccgatcacga
      841 ccgcggcgct gcccaccacc aagccaagcg ttgcgatgcc gatggccacg cagagttgca
      901 gccaatacga ggtgacttca ctcgcgtccc gacggagcaa attcgcgacg agcagcgagc
      961 gcccttcgac actgttgcca agcagccgga ccactcggtc ttgaagccca gccatgggtc
     1021 gtggatgcgt ggtgttcata acctcgaatt ggtacacgcc ggaaggctcc aggatccacg
     1081 ccttttggat tggccgacct cctcgagcgg cgctcgcaga agtcaggtag acggcgcgac
     1141 ttcgtcgagc cccccgtgcg atgctcgccg cgtcatataa cccagctcgt atcacgcgac
     1201 tacttccgta atgctcgatg ggatgcaggg ttaggcgccg acgagccctg cgttcgaaga
     1261 ttggttggat ggtcctcctg gacctactct tgaggttgga ccgccttgat cagcgcctcg
     1321 agctcttcct tgccgtgcga caggcccagc tttccgaatg ccttcaatag gctcgccagg
     1381 acctcggggt tgggggccag gatggcggcc attgccgcgc tgttctcctg aaccgaagcc
     1441 aagaatgcga gacgcgcgcg acggttcttc gagacctctt tcatgagctc gcgcattacg
     1501 gcccgcagtg tttcttcgcg ttgagtgacg accatggcgg caagctgagg gcgctcttgc
     1561 attgcctccg ctgccgcccc cagtgatgcg ggcttgtcgc tcgctgcgtc cagggtcgca
     1621 atcaagacct gcttcaggcc cgcggggcgc tcgacgagtc gccgtgccgt ctgccgcgag
     1681 agctcgtctt gctccagccc cgcggcggta ttctgcagaa agcggtcgag agtcttggga
     1741 tgtttgagtg tcagcgcgaa caactcgtcc acatactcgg gatgttcatc cgtaacgcgc
     1801 agagtggcct cgagcgattc gtggcgaagc tgtgggttat cgagcgccgt gtcgagcgcg
     1861 ggcttttgcg tgctcgcact acaagcaacg agcagtagac tcgctaacgt cccgagccat
     1921 aacgccccgc gcgccacgat cgggcgcgaa ctgcgaggtc gatccaagcg agcagggcta
     1981 cgcccaatcg tggtgcgcct cggcgcgatc gggtacaccg ggcgcgggtt agcgttttcg
     2041 ccaagacaac cgtggaccga gcgcggcgag ttctgaggca tatctggctt tcctcgatgg
     2101 cgccccgtcg ggcgcaggag gttcacgagc ctgcactccg tgtgccagct ctgaatgcca
     2161 actcgttctg aacagccgga gctaggccaa gcttactgga tgttcttgga cttcatctcc
     2221 catgcggagt cgctgcagta agggcagccg cttccaacga gcaagccctt gccggtggcg
     2281 ccttcgtccc ccttcaggtg ccagtttagc caagccaaat cgatcttggt gaagtctcca
     2341 ccgttcctcg agaacaaatc tccgccgtgc ccaagcgcct tgctgaacaa gagcgtggga
     2401 atccccagcg cgctgatgtt gtcgaaatct ctaagtccat tctcgtaggc gatgtcgtcc
     2461 ggaccaccga gcacgattag aacgggagtg tgaatggttt tgtaaaagga ctgattcgca
     2521 ccgaagagac cgctgctgtt gatgccccag gtcgtaatcc ggggatcgcc cgccgtaccc
     2581 tcggccatca acccgccaca cgagaacccg ttggcggcta ccttcgtcgt gtcgaggctc
     2641 tggtaatacg cgctgcaagc tttgtcgttc tcggcaatgg cccaggtgac gtatccgagt
     2701 aggggcttgc ccatcgcggc tgggtttgcg ccgtccatgc tgcgcgaatt gctggcattc
     2761 ggtgtgccgt ccgcaatcac gaagtatccg tgggaagcga tctctgccat tgccgcggaa
     2821 ttcgagaggc cgttacgtga acagccacct tctccccaaa cgaagattgg gtatttctcg
     2881 gctcccccta agtcggcggg acggaaaatc gtgccttcgt tgatgccggc gtcagaattg
     2941 gtttcgacaa ccaccttgtg tggtccgctg cccgatgcgt tcgtgttgac ggacttcgag
     3001 gcaacgcagg tgccgctatt cgcgctgcca gcggcgcccg aagctcccgc gggagttccc
     3061 ccgctcccag cactgcctcc ctcgcttccg cgtccggcgt tgccggccga gtttgctcgg
     3121 cccgcgttac ccccgggctc cccgcgaccc ccagaagcgg cgccagccga gccggacgtc
     3181 ccgttgttcg aaccaccact gctgggcgcc cctgcggaaa tgaccccacc ggccgcgttc
     3241 gtcgtcgatg atagcccgcc actaccccca acgttcgtgc cctgtcctcc ggcaaaggct
     3301 cctccggtaa actgtcctcc aaaactcgaa gccattcccc cgctggcggg cacgacgcct
     3361 ggattcgacg agccgccgtt cggcgagacg ccaggcccac tcccacctgg atccgaattg
     3421 ccgcaattgg caaggatcag aacgctggcg cctagcgtga acagatgctt agacctcatg
     3481 aagaaaatca ctcctcgtcc gggctacgtg gggctcgctc tgctgcggca tgctgcgctc
     3541 gaaactggtc cataaaagag cgcgcccgag catgatcgcc atcccgcatc agcaggatca
     3601 gaaggatccg ccgtgactcg agttgccggc tttggactag gagacgcgac tacagagcct
     3661 ggctacagcg cgtggcgctt gagcacaggc catgcaatct cggcatcgcc tggatcatcg
     3721 cgaccgccgg acttcgtgca caacgctact tcggcgtcgc cgtcgcagcg cgagtatgtc
     3781 gaacaaccgc cgtcatccac ggtcggagcg gagtccgagc aatgatggat atcggcccat
     3841 ctttcgaaag ccaaaaaagt gatcggcatt cccggcacca gggatgaagg gccaccgccg
     3901 tacggaactc ggggatcggc cgtgccccga aacgagatct cggtgatagg ccgtcgcggg
     3961 atgcactcat cgacgttctc ttccaggaaa tcgaaggcag cgggcgccac ggccgcaaac
     4021 aagtccgcgg cgtgacaagc aagctggtag accatgcccc cgcccgtcag gacacccacc
     4081 gcataaactc tgcgggggtc tatacacgcg ctctgttgca ctcgggccac gagcgcccgc
     4141 gcgaaagcga gatcgtcgac gtcggccaca caacaaggtc ctaagttcca ggccgtgccg
     4201 agtggcccct tgagtccatc cggaaaggcc atcaccacac cttcgcggtc ggttaccgca
     4261 ggataagggg agctccgcaa ctcactcata ccggactccc ccatcccgtg gaagtcgacg
     4321 atgagtggag cgggcgtcgt cccatcgtaa ctttcaggaa catgcaatac gaagctgcga
//
LOCUS       DASTKU010000118         7929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_913744, whole genome shotgun sequence.
ACCESSION   DASTKU010000118 DASTKU010000000
VERSION     DASTKU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913744"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="VFQ61_04130"
     CDS             complement(<1..1439)
                     /locus_tag="VFQ61_04130"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9953663.1"
                     /translation="MSAPKPRSSKGPLHVGLSALLAIYGCATPEEQQRAFGPSTPVPT
                     ASSTSPVPSPSSSSVPSPSNTAPPELPSGASPPQPWTAPGLSLFDDPTSCEAQNLSAG
                     RSRLRRLTRLEYNNSVRDLLGDDSRPANAFVADQKVLGFNNNAYAAAGTTIAREYVLT
                     AETLARAAVSSNLSSLMDCGPEPSDACISEFIRRFALRAFRAPIEPAHFARLQTLYLD
                     TSTRFGLAAGIEAVLTSILSSPRFLYVLEYGADSIAPLEAPNRAVSQYELAARLSFYL
                     WRSVPDLELLELAGLGELADASRLQTQAERMLRDPRAISAVQDFAEQWLELDGLEGIT
                     KDSNLGFAWTPEVARALRTESLLTYSNAVLSEGSTLAQLLLSPTSYINGELGEYYAVP
                     VSNLVFGRRDVNPDPARPIRLGLLGQGGVLARHAHATLASPVLRGKLVRDKFLCRPVP
                     PPDSAIVGIIPPAPNTHVVGKTTRDLVTA"
     gene            complement(1473..3305)
                     /locus_tag="VFQ61_04135"
     CDS             complement(1473..3305)
                     /locus_tag="VFQ61_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953664.1"
                     /translation="MKYRALVGLLLPILACSSKETNPVGGGGQSPGIGGATASGGTSN
                     SAGSGGSTSPAGASQGGSTTSMSGGQTASGGGNNTSSGGKATTGGASVGGSSGGTTSM
                     PTGGATSDDGGSGGVAGGAAGGTPSASDSVLERNNSPSRDGHFVRPDITRAKAAEMMP
                     DAAFKATFKGSMFASPLYAADGPGGKGAFFAATTGNDVFALDATTGATLWTKNIGSAP
                     SATGASGALCGNIRPVGILSTPVIDPTTRTIYVAGAIGTTRIERHEIHALSLDDGKER
                     DGWPIDATKIAAGTVKMDPVQPYNQRSALSLVNGVLYVAYGGHIGDCPVYHGWVTAID
                     TKDPTKTGAWATAGEGEAIWAAGGLASDGSSVYAATGNSTNGVSDRTKSDSEAVVKLT
                     GLAKFERNNANTFYPANWRTLDQQDADLGGSNPVFFSLGASKYLATIGKNGHMFLLDP
                     ANLGGSDGHKVDFQIADGGAMAVKTVPTVYKSAQGVNIAFSVDSSAVGCPAGGPMGAR
                     MTKMVMSVSIPQGSPPSPKVAWCAALSGNSTAPITTTTDGSADSIVWYVNNGKLSGVN
                     GDTGQVIYTSSDTCSGVQRWTSPIAVGKRVVAGGDGKLCAWTVK"
     gene            3910..4884
                     /locus_tag="VFQ61_04140"
     CDS             3910..4884
                     /locus_tag="VFQ61_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953665.1"
                     /translation="MEAPPEKLSQTGCVKAGDPKQFADIVLPYEVNSPLWSDASDKSR
                     GLVIPAGKQIHVVDCSKDTCAQGVYDDGKWQLPVGSVAIKNFGFDGKLVETRLFVRFD
                     EKNYVGYSYRWDEAQTDATIVPDERVTVMFNTGKRTVEWNYPNRLDCSTCHNNQAFGF
                     IGFETQQMNRVVGGTNQIDALKSKNVFEAPPKSPYAAALVAPYPNQLGSPPATATLDE
                     RARSYLHGNCAYCHRPDGDFQNHDLRFGVPMKDMGICNSEPVKGDLGVTGAVNLSPGD
                     PKNSIMYLRMQVRRQAKIGMPQLGTYVVDPDGSELIGQWITSIKSCPQ"
     gene            complement(5031..6068)
                     /locus_tag="VFQ61_04145"
     CDS             complement(5031..6068)
                     /locus_tag="VFQ61_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953666.1"
                     /translation="MTLFTGRWIGVPLVLLLGFACNSEPSSGSSTTGQTGGVGQGGSS
                     QPGQGGASQTGGTLENLGATSTYGGTAVSSGGAPTSGGATAQGGTPDAGGGTTSGGAA
                     NAGGAPSGGSAGGTAAGGTVYAGTNSGGASSSGESGGTAGAGAGGSSIAGGSGGGGSA
                     AHAMMNFFVTSDSKTDANLGGLAGADQRCQRLAAAVGHGSKTWHAYLSVGSPMTHAKD
                     RIGPGPYYNSKGELLASDKTALHARSGDAALFLDEKGNRVNGQWAGSPTPNQHDILTG
                     SQRDGTLATGMTCGDWTSTTGSSQVGHSDGLGPGMQMGMYVYWNSVHTGQCGDTAPGG
                     GAGKLYCFTAE"
     gene            complement(6579..6651)
                     /locus_tag="VFQ61_04150"
     tRNA            complement(6579..6651)
                     /locus_tag="VFQ61_04150"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6616..6618),aa:Phe,seq:gaa)
     gene            6791..>7929
                     /locus_tag="VFQ61_04155"
     CDS             6791..>7929
                     /locus_tag="VFQ61_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET9953667.1"
                     /translation="MVLSSPKPILVELGSRDSERRMNRRPRLGYAGRIVFSSQPGGQA
                     NPYESEGYLQQLLQKAIAAGARDVHLKVGQPPGARVRGSLVFFRVDRIRPEDTLAVAR
                     HLIRDPQVLANLDNLREYDTAYAIPGVGRFRVNVYRQRASLAVVMRVIPARVPSLEQL
                     GTPLVCRMLAEKDRGLVLCVGAAGNGKSTTLAAMIDHMNHTLSRHIVTIEDPIEYLYE
                     DDRSSISQREIGHDTQSFASALRAALRQDPDVIQVGEIRDAETMEIALKAAETGHLVL
                     STLHTPDVSRTMNRIIALSQGESEDLRDRLADALQGIIAQRLLPRADAQGMVLAAEVL
                     VGTGSVRETIRRPLGNPSLKELMESGAEMYGMQTFEMSVRQLVREG"
BASE COUNT         1492 a         2396 c         2477 g         1564 t
ORIGIN      
        1 tgggcagtga ccagatctcg agtggtcttg ccgacgacgt gggtattagg cgcaggcgga
       61 atgattccaa cgattgccga gtcgggcgga ggaaccgggc ggcacaggaa cttgtcacgc
      121 acgagtttgc cgcgcagcac cggcgaagcg agggtggcgt gggcatggcg agccaacacc
      181 cctccttgcc cgagcagccc cagacggatt gggcgggccg gatcggggtt cacgtcgcgc
      241 ctgccgaaca ccaggttgct caccggaact gcgtaatact ctccaagctc gccgttgatg
      301 tagctcgtgg gagacagcag cagttgagca agagtgctac cctcgctcaa cacggcgttg
      361 ctataagtga gcaggctttc ggtgcggagg gctcgcgcga cttcgggcgt ccaagcaaaa
      421 ccgaggttcg agtccttcgt aatcccctcg aggccgtcga gctctagcca ttgttcggca
      481 aaatcctgca ccgcgctaat cgcgcgcgga tctcggagca tgcgttcggc ttgggtttgg
      541 agtcgggaag catcggccaa ttcacccagc cctgctagct ccaggagctc cagatcgggc
      601 accgatcgcc aaagatagaa agagaggcga gcggcaagct cgtattgcga gaccgctcga
      661 ttgggtgctt caagtggtgc gatcgagtcg gcgccgtact cgagcacgta gaggaatcgc
      721 ggtgaactga ggatcgatgt tagaaccgcc tcgatacccg cggcgaggcc aaatcgagtg
      781 ctggtatcta gatatagagt ttgaagtcgc gcgaagtggg cgggttcgat gggagctcgg
      841 aacgcgcgca gtgcgaaacg ccgaatgaac tctgatatac aggcgtcgct cggctccgga
      901 ccacaatcca tcaagctcga tagattggag ctaaccgccg cccgggccaa ggtttcggcg
      961 gtcaacacgt actctctggc gatcgtagtt ccagcagctg cgtaggcgtt gttgttgaat
     1021 cccagtacct tttgatcggc gacgaaggca ttggcagggc gcgagtcatc gccgagtaag
     1081 tcacgcacgc tattgttgta ttcgagccgc gtgaggcgcc ggagtcgcga gcgtccggcg
     1141 ctcagattct gagcttcgca gctggtgggg tcgtcaaata gcgagaggcc cggagcggtc
     1201 cagggttgtg gcggactcgc gcccgagggt agctcaggag gcgcggtgtt cgagggcgac
     1261 ggcacactcg acgaactcgg gctcggaacg ggtgaggtcg agcttgcggt tgggaccggt
     1321 gtggaggggc cgaatgccct ctgctgctct tcgggagtcg cacagccata gatggccagc
     1381 aacgccgaaa ggcccacgtg gagtgggcct ttcgaggagc gaggtttggg cgcgctcacg
     1441 cctgagctcc gtgtggagtg gacctcaaag tgtcacttga cggtccacgc acagagcttg
     1501 ccgtcaccgc cagcgaccac gcgcttgccg accgcgatcg gggacgtcca acgttgcact
     1561 ccagagcagg tatcactgct cgtgtagatc acttgtccgg tgtctccgtt gacgccgctg
     1621 agcttgccgt tgttcacgta ccaaacgatg gagtctgcgc tgccatccgt ggttgtcgtg
     1681 atgggggcgg tgctgttgcc cgagagcgcc gcgcaccaag ccaccttggg cgacggcggc
     1741 gagccctgcg ggatcgatac cgacatcacc atcttcgtca tccgggcgcc catgggacca
     1801 cctgctgggc aacccactgc gctcgagtcg acggagaagg cgatgttcac gccctgggca
     1861 ctcttgtaaa ccgtgggtac ggtcttcacc gccatcgctc cgccgtccgc aatttggaag
     1921 tccactttat gcccgtcact cccaccgaga ttcgcgggat cgagtaggaa catgtgccca
     1981 ttcttaccga tggtggccag atacttagac gccccgagcg agaagaacac aggattgcta
     2041 ccgccgagat cggcgtcctg ctggtccagt gtacgccagt tcgcgggata aaacgtattc
     2101 gcattgtttc gctcgaactt ggctagacca gtgagcttga ccaccgcttc ggagtcgctc
     2161 ttcgtacgat cggaaacgcc gttcgtgctg ttacccgtgg ccgcgtatac gctgctcccg
     2221 tcagatgcca agccgccggc ggcccagatc gcttcacctt ctccggcggt cgcccacgcc
     2281 ccggtcttgg tcgggtcctt ggtgtcaatg gccgtaaccc agccgtgata gacaggacag
     2341 tctccgatgt ggccgccata agcgacgtag agaaccccgt ttacgagcga cagcgcactc
     2401 cgttgattgt acggttgaac cggatccatc ttgacggtgc cagcggcgat cttggtggcg
     2461 tcgatgggcc atccgtcacg ctctttgccg tcatcgaggg acagcgcgtg gatttcgtgc
     2521 cgctcgatac gcgtggtgcc aatcgcgccc gcgacatata tggtgcgtgt cgtcgggtca
     2581 atcaccggcg tgcttagaat tccgaccggg cgaatgttgc cgcaaagcgc acccgaagcg
     2641 cctgtggcgc tcggagccga gccgatattc ttggtccaca aagtggctcc ggtcgtcgcg
     2701 tccaacgcga agacatcgtt gcctgtcgta gcggcgaaga aagcgccctt tccgccagga
     2761 ccatccgcgg cgtaaagagg ggacgcgaac atgctgccct tgaacgtcgc cttgaatgcc
     2821 gcatccggca tcatttccgc tgctttcgcg cgggtaatgt cgggccgtac gaagtgacca
     2881 tctcgtgagg ggctgttgtt gcgctcgagt accgagtcac tcgcggacgg cgtcccgccc
     2941 gccgcacccc cggccacgcc gccactgcca ccatcgtcag aagtagcgcc gcccgttggc
     3001 atgctcgttg tcccgccgct cgagcctcca acgctcgcgc ctccggtcgt agctttgcca
     3061 ccgcttgaag tattgtttcc gccgccgctc gcggtttgac ctccgctcat ggatgtcgtg
     3121 gagccaccct gcgacgcacc ggcgggactc gttgagccac ccgatcctgc actattgctg
     3181 gtgccaccag acgccgtcgc tccgccaatt cctgggctct gcccaccacc acccacggga
     3241 ttcgtttctt tcgatgaaca ggcaaggatc ggcagaagta gaccgaccag cgcacggtat
     3301 ttcatgacat aaccttcttt ggggaagtga ttaggagagt aatcacaagc gtcgtggatc
     3361 actctccaca tttgcgctac tagctttacg ccactacgct ttgcaagcgt gctggcgctg
     3421 ggacttgaag tcaacccgca tagtgtctcg tctagcttcc gtcccttgtt accagtctaa
     3481 gccaaaattc caatgagaat acgcatgcgt tcccagctgc aatacttgcc agttaacttt
     3541 gtttgcttgt tggttgcgat cgcctgcggg gcagagaagc cagatggcgt gagtgctgga
     3601 ggcgcaccac ctctgacgtc tggtggcgcg aacaactccg gcggagtgtc gccacaaggt
     3661 ggcagttctg tgtcgggatc caatagtgta actggcggaa ttaccgcagg cggagccgtc
     3721 tcaggcggcg cggcaaacaa aggtggttct tcgaacaacc agggtaacgc gggtgctggc
     3781 ggagcaggca acgccggggc gagcggcggt tccagcggaa actctagcca agccggagct
     3841 ggccaagcaa cgggaggcag tagccggggc ggggcaggcg ccctgggtgc ttgcacgcct
     3901 ccggaaaaaa tggaggcacc cccagaaaaa ttgagccaaa ccggctgcgt caaggcagga
     3961 gatccgaagc agtttgcaga tatcgtgttg ccctacgagg tcaatagtcc tttgtggtcc
     4021 gacgcttcgg acaaatcacg gggtctggtc attcctgccg gcaagcagat ccacgtcgtt
     4081 gactgcagta aagacacttg tgctcagggt gtctatgatg acggtaagtg gcagctgccc
     4141 gtcggctcgg tcgcgatcaa gaacttcgga ttcgacggca agctggtcga aacccggctg
     4201 ttcgtgcggt tcgacgagaa gaactatgtg ggttactcct atcggtggga cgaagcgcaa
     4261 acagacgcca ccatcgttcc ggacgagcgt gtcacggtga tgttcaacac cggcaagcga
     4321 accgtggaat ggaactaccc gaatcggctc gactgctcaa cgtgtcacaa caatcaagct
     4381 ttcggcttca tcgggtttga aactcagcag atgaatcgag ttgtgggtgg cacaaatcag
     4441 attgatgccc tcaaatcgaa aaacgtattt gaggctcccc caaaaagccc gtacgccgca
     4501 gcattagtag ctccctatcc caatcagctc ggctcaccgc cggccaccgc cacgctggac
     4561 gagcgagcgc gctcgtacct tcacggaaac tgcgcctact gtcatcgtcc ggacggagat
     4621 ttccagaatc acgacctccg gtttggcgtg cccatgaagg acatggggat ctgtaactcg
     4681 gagccggtca aaggtgacct cggagtcacc ggtgcggtga acctctcgcc gggcgatccg
     4741 aagaactcga tcatgtattt gagaatgcag gtgagaaggc aagcaaagat cggcatgcct
     4801 cagctcggaa catacgtggt tgatccagac ggatccgagc tgattggtca gtggatcaca
     4861 tcgataaaat cgtgtcctca gtgaacagtg caacccgttt gtctggcgtc cggaccttcg
     4921 gagagcggga gtaggatggc ccgggttgca gccgatgaat gctcccgggc ctagcgctcg
     4981 ggcgcttcgc tctcagcggc cctctccgca ctcgttcgaa tctagcgggc tcactccgcc
     5041 gtgaagcagt agagcttccc ggcgccgccg ccaggtgcgg tgtcgccgca ctggccggta
     5101 tgtacggagt tccaatatac gtacatgccc atctgcatgc cggggcccaa accgtcggag
     5161 tgtccaactt gagagctgcc ggtcgtcgag gtccagtcac cgcaggtcat tcccgtggcg
     5221 agcgttccgt ctcgttgcga accagtcagg atgtcgtgct ggttgggagt gggtgagccc
     5281 gcccactgac cgttgacgcg gttgcccttt tcatccaaaa acaacgcagc atcaccgctc
     5341 ctcgcgtgaa gtgccgtctt gtccgaggcc agcagctctc ctttggagtt gtagtagggg
     5401 cctgggccga tgcggtcttt ggcatgagtc attggggagc ccacactcag gtaggcgtgc
     5461 catgtcttgc tgccgtgacc gaccgccgcg gcgagccttt ggcagcgttg atcggcgcct
     5521 gctaagccgc cgaggttcgc gtcggtcttc gagtcgctcg tgacgaagaa gttcatcatc
     5581 gcgtgcgcag cggagccgcc gcctccgctg ccccctgcga tgctcgagcc accggccccg
     5641 gcgcccgcgg taccgcccga ttctccactc gaggaggcgc cacctgagtt cgtccccgca
     5701 tatacggtac cgcctgcagc cgtcccgcct gcacttcctc cggaaggcgc gcccccagcg
     5761 ttcgctgcgc cgccggatgt ggtaccgcca ccggcgtcgg gcgttccgcc ttgggcagtg
     5821 gctccgccgg aagttggggc tccgccgctg ctcaccgctg ttcccccgta cgtcgaggtc
     5881 gcgcccaagt tttccagcgt tccgccggtt tgggaggcgc ctccctgacc cggttgcgat
     5941 gaccctcctt ggccaacgcc tcccgtttga ccagtcgtac tcgacccact cgacggctcg
     6001 ctgttgcagg cgaatccgag cagtaggacg agcggcactc caatccaacg accagtaaac
     6061 aaagtcatag gttctccctc ggtcagtgcg atgcgaatcg caactgggcg cagttcgttc
     6121 acagcaatct tagctgccat gaacgtgggg cctactgtga caatcgtgat ttacgggtct
     6181 gctttgaaac tggcgggtaa cgttcgtctg ggtcccgcgt aaagtcgagc tcgcgtgtct
     6241 cgtatgcgtc agaagcccca ccgccgattc cgctcacgcg ctcgatgaga gtcagctgcg
     6301 gctcgaaaga atgaaactcg acggaatgag cagaacgagc agagtgagca ggtgattgaa
     6361 gccctggacc caatcgaccc ccacttcgtg tgacgggctc gtcgcgacag gggactgagc
     6421 cggtgctcgg gcctcgaaga gcagcgtatg cagagctgcc agcctgcgcc ggcggcgtgc
     6481 tctgtcgtta agagcacctg tcaacagcgc cgcaccgcga gaaaaatcga tctcctagaa
     6541 gatctcggat ctctcgcagt ggttgcgttg gataccggtg tccggggacg gaattgaacc
     6601 gccgacacga ggattttcaa tcctctgctc taccgactga gctacccgga cggagattcg
     6661 tacggtcgga aacccgttgt tcccgagcga cgaggggggc tttctagctc agggtggggg
     6721 aggtctgcaa cctcctcgcg cacacgggcg acacgttttt tgtgtttctg tgtggtcggc
     6781 cggtttgccc ttggtcctct cgagtccaaa gcccatcctc gtcgagctcg gcagccggga
     6841 ttccgagcgt cggatgaatc ggcggccgcg actggggtat gctggtcgca tcgtgttcag
     6901 ctcgcagccc ggaggccaag cgaacccgta cgaatccgag ggttacctgc agcagctgct
     6961 gcagaaggcc atcgccgcag gcgcacgcga tgtgcatttg aaagttggtc agcctcccgg
     7021 tgcgcgggtg cgcggcagcc tggtgttttt tcgcgtcgat cgaattcgtc cagaggacac
     7081 gctcgccgtc gcccgacatt tgatccgtga cccgcaagtg ctcgccaacc tcgataactt
     7141 gcgggagtac gatacggcct atgcaatccc gggggtgggg cgcttccgcg tcaacgtgta
     7201 tcgccagcgc gcgtctctgg cggtcgttat gcgcgtgatc ccggcacgcg tgccgagttt
     7261 ggagcagctg ggaactcctt tggtgtgtcg gatgctagcg gagaaagatc gcggcctcgt
     7321 gctgtgcgtc ggcgctgcag gtaacgggaa gagcacgacc ttggccgcga tgatcgacca
     7381 catgaaccac acgctgtcgc ggcacatcgt gacgatcgaa gatccgatcg agtacctcta
     7441 cgaagacgat cgatcgtcga tcagccagcg cgagattggt cacgataccc agagctttgc
     7501 cagcgcgctg cgcgccgctc tgcgtcagga tccagacgtc atccaagtgg gcgagattcg
     7561 tgacgccgag accatggaga tcgccctgaa ggccgccgaa actgggcacc tggtactctc
     7621 caccctgcat actcccgacg tgagccgcac gatgaaccgc atcatcgctc tgtcccaggg
     7681 cgagtccgag gatctgcggg accgcctggc tgacgcgctg caaggtatca tcgctcagcg
     7741 tctgttacct cgcgcagacg ctcagggcat ggtgctggca gcagaggtgc tggtgggcac
     7801 cggctcggtt cgggagacca ttcggcgacc gctcggcaac ccgtccttga aggagctgat
     7861 ggaatccggg gcggagatgt acggcatgca aaccttcgag atgtcggtgc gtcagctcgt
     7921 tcgcgaggg
//
LOCUS       DASTKU010000119        30222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_913924, whole genome shotgun sequence.
ACCESSION   DASTKU010000119 DASTKU010000000
VERSION     DASTKU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 30222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30222
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913924"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            458..2932
                     /locus_tag="VFQ61_04160"
     CDS             458..2932
                     /locus_tag="VFQ61_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9953668.1"
                     /translation="MFATDLALSIKATEYWWAIMSYGVRLSLLALSFATLASSSACSS
                     NEGSSNRGGGGRSGGGAAGTGLSPGTGNSPGTGNSPGTIPIPRPPGGNCGNGKQDSDE
                     LCDDANKEAGDGCNPACRVEADFECPTFGQPCVSKAICGDGKLASTETCDDMNTVAGD
                     GCSANCQAVEPGWQCRVPGQHCIPLCGDSVITAPENCDDANATSGDGCSSTCLTEPGY
                     DCKAPGQPCVKSVCGNGVTEAGETCDAGMNNGLFFGDGKGCSKTCTLEPTCRDDKGVT
                     TACKTPCGDGNKDPGEECDDGNGSNGDGCSKECKVEVDAGFACEDKVIKDTQPCSDGA
                     GECLVLPIVYRDFDGQNQPTGHPDFFYLSSGTTKCVPNASGNKDTTTTAGDCWKSDST
                     DLCSGLVEATLGADGKPVYSATKAGTCQCRFTDWNQTTVVPAGTIPAGTACTVRDNNG
                     GAQGTRNRIEANVQAIQSAASFKDWYSDSAKSTKVTDKLELKLLAGNQYQFSSSNGRT
                     VYEDIHDIFLASKGEPALPGAVTSLTSGFFPLEAQTRPKVCNIWPYWVADGQSSCASG
                     ATLDVRNQWDPKGWYEGTKPANPMGGEVASVTGVKRNFYFTSEVRYLFRYAGGEVLSF
                     YGDDDVWVFINGKLALDLGAPHERLQGSVTLTPTGANWTINTAAKNVTGTVQLGTFEQ
                     GKTYEIVVFHADRHPRESNYQLTLSGFSTTQSVCKANCGDGKVAAGEECDNGAMNQDG
                     LYGGCTTQCKFGPFCGDKEVNGTEECDLGKDNGAQYGQEGCTKACTLPHRCGDGIIDS
                     KFNEACDDGPNNGKGPCNPDCTFVPL"
     gene            3104..5662
                     /locus_tag="VFQ61_04165"
     CDS             3104..5662
                     /locus_tag="VFQ61_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HET9953669.1"
                     /translation="MKNSGRGRGPDHQKPVEPTPAELGTEAASPERGRDAALTHRTVD
                     ATLAAEWLWGWDATPGIVSVWAEMDGRVWVWRRSPETLELVQEHVRFRPWLLLGSLRD
                     LEHLQNRLVPSSSKHARERGHFSYHELNGPGELRYWVSAADGRDLTRAVLRGASERLG
                     YEVRDVRELAPDVVLLSPEEQYLTRSGRTYFRGLSFDQVRRMHLDLETTGLDAEVDRI
                     FLVALRMPDGRAEVLRGESHDDVGEAALLRKTLARIAEYDPDVLENHNLHGFDVPFLV
                     RRCQHLGIKPDLGRLPGLKKRAAARGMVNALSTAAGPQARPRDSEEMLSRSPTSLSRR
                     ARFSVPGRELLDTLDAVLRYDFAARDLPGHGLKEVARHLGLAQSDREYLPAAQAFSLF
                     QHDPERVMRYARDDVQEAAAVGRLLGGAAFGLAQMAPRRYERLADAGPATGVLDPMLL
                     RAYLHSDTALPRPSGGDGTPHTGAALYLFATGVAQNIVKADVASLYPSLMRQYRIGPQ
                     TDVLGALLTLVEHWVEQRLRAKSLAKTCPPHSPERFGHEALAAALKLLVNSAYGYLGA
                     VGLTRFSDVHAANEVTRHGRELLDLLCQELAERGVTLLEADTDGVYFSVPPGTTEAQE
                     RGIVEQVAALLPEQVHLEFDGRYKTMLSHEPKNYALLGYDDQLILRGVAFRSRRAEPF
                     GELFLERALRALLSGDIEGVRQSFIDTLRALRRRELPTGAVAALVRLTKTPEQYLAAR
                     EKRRELPYEALIGRGRFQWSVGERIRVYRTQGGGAGLFDEEASEDARDYHVVYYARLL
                     RDTFAARLARAFSREDYELLFADPTQLSLFERPIRSVRPILTTGVSSLPATRAR"
     gene            complement(5668..7089)
                     /gene="nhaA"
                     /locus_tag="VFQ61_04170"
     CDS             complement(5668..7089)
                     /gene="nhaA"
                     /locus_tag="VFQ61_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET9953670.1"
                     /translation="MQSTDRLNRSPSPSPPEAWSPIRRLGLLATRPLERFLHIEAASG
                     ILLLLAAAVALLWANSSWQHGYHELFRTPLGVRIGGFSFERSLEWVVNDGLMVIFFFV
                     VGLEIRREIHQGELSEWRRAALPAAAALGGMLAPAGIYLLIAGAPKTHSGWGVPMATD
                     IAFALGVLTLLGQRVPPALRVLLLALAVIDDLGAILVIALFYSSGVQWVGLAVAALGL
                     LSILLLQWLGVRNKLAYIAPGLVAWGGTYAAGIHPTIAGVAIGLMTPVRAWYGAEGFV
                     RGLQEKIEPLAAERDVSAVSPGQLSEVLRDVNVAHREARSPAESLIETLHPWVAFIIM
                     PLFALANAGVTLSGVRFGEHETAVLLGAAAGLIVGKPVGILIACALVLKLGLATLPRG
                     LGKAHLVVLGMVAGIGFTMALFVAQLAFDDAGLLGAAKLGILCASGLAGILGLAVGAV
                     ALARKHAPGAAPTADDAECSTEA"
     gene            7479..8909
                     /locus_tag="VFQ61_04175"
     CDS             7479..8909
                     /locus_tag="VFQ61_04175"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9953671.1"
                     /translation="MTSPQSRDVVTPEGCQAQGQAPGIAPEGTAAPASTPGSRAAAGK
                     TTEHSDVLAPEARRALLDAPRPRSLPIAPNAPRALRHLPLAESARDVDREARPIYAVW
                     EITLRCDLACRHCGSRAGHARSDELSTSECLDLVAQMADLGVLEVTLIGGEAYLRDDW
                     TEIVSAIKARGLSVTMTTGGRGITAERAEQASRAGLESASISLDGLAETHDRLRGVRG
                     SFESALQAMDHLRRAGVKVSCNSQINRLSLPELAPLLELIAERGAHSWQWAMTVPMGR
                     AADEPDVLLQPYELLEVFPELARVHRRAEELSVKIWPGNNVGYFGPYEHELRRYSPRG
                     HASSCAAGRLVLGIEADGSIKGCPSLPTAAWTGGNIRDQSLRDIWERSKPLRYTRDRT
                     VEDLWGFCRSCYYADECRAGCTWTSFVFFGRAGNNPYCHHRALEMQRQGLRERVRRIQ
                     AAPGEPFDHGNFEIVVEPNPSLEPSA"
     gene            8906..9559
                     /locus_tag="VFQ61_04180"
     CDS             8906..9559
                     /locus_tag="VFQ61_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953672.1"
                     /translation="MTRLFACPSCHRHVKVDETQCPFCSTAFSSTEHPERPVPTQRMS
                     RAASFAFSATVAAGTFAAACSGQTEGTPVPVYGAPAGGVGAMTSTGGSSGAGGQAGGS
                     ATSGGNPSSGGATNTGGRTSSGGQVSTGGASMATGGRTGSGGKASGGAGPDPFGGAIA
                     VYGAPPPTGGRSNASGGANGEAGSVGEAGAAGDVGTAGATDPGTLPVPLYGAPANLK"
     gene            complement(9735..11630)
                     /gene="dps"
                     /locus_tag="VFQ61_04185"
                     /gene_synonym="pexB"
     CDS             complement(9735..11630)
                     /gene="dps"
                     /locus_tag="VFQ61_04185"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HET9953673.1"
                     /translation="MSDPDRAAEREQHPLGLLVQWTTLGVAVGLFAGVASALFLWLLE
                     RATQYRTGHHGLVWALPVAGLTLGWFYERYGQPIRAGNNLVIDRLHDAGPELPLRMGP
                     MVLAGTVLTHLFGGSAGREGTAVQMGASLADWLSHRLKSTKVLRRQLLAAGVAGGFGS
                     VFGTPIAGTVFGLEFVLLGRIEYHALWPALVAALIGDFTTRALGIEHTAFPVLAETQL
                     SPALFLKWILFAAAVALTTTLFIELTHWLKQQSERRLPRLGWRMAVGGVAVIALWQAS
                     GTDDFLGLGVPVIQRAFVDGSLPLSTWFLKLVFTAVTLGTGFLGGEVTPLFFIGATLG
                     NALARGLGLPLDLAAGVGLASVFAAASNTPIALAIMALELLGARIFPHVMIVNVLAYL
                     MTGHRSIYPAQRVLFSKVGAQVSGPLPLRELQHSRRKSTQKPDDTSDSRTWTRDGASE
                     NATGRGLDICIRGRDIPRQENPKMYKSPSHLPEQARVSLCETLNARLADGLDLHSQIK
                     VAHWNIKGPQFAALHPLFETFAVSLATHNDTIAERSVTLGGKAYGTARHVAKQSQLPE
                     YPQDTTRDMDHVRLLAERIEKYLDVVRESRSNAEKLGDTDTVDLFTQIVTEFEKHAWF
                     LRASLES"
     gene            complement(11717..12967)
                     /locus_tag="VFQ61_04190"
     CDS             complement(11717..12967)
                     /locus_tag="VFQ61_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9953674.1"
                     /translation="MTSELIESVQHLLRELAPQVLSRVLRRCGDFSAAEDAVQEALLA
                     AATQWPREGTPENPRAWLVRVAMRRLTDQVRADSARKHREALVVSLIPPDEQIAIAPD
                     ELFGTSEDDTLDLLFMCCHPCLTEASAIALTLRAIGGLTTPEIARAFLVPEGTMAQRI
                     SRAKQSIQSSGVPFERPTEQGRAARMQAVAHVLYLIFNEGYTASSGDELHRTDLSQEA
                     IRVTRLLLSLAPDEPEVAGLLALMLLTDARRPARSGPRGELIPLDEQDRSLWDQNRIA
                     EGTELLTRTLPRGATGPYQLLAAIAALHDEAARAEDTDWPQILALYSVLLEISDNPMV
                     ALNHAIALAMVHGAAAGLERLEKVGTDPRLREHYRLDAVRAHLLERTGDPRGAAALYR
                     RAAARTTSRVEQEYLLERAGRIES"
     gene            complement(13016..13408)
                     /locus_tag="VFQ61_04195"
     CDS             complement(13016..13408)
                     /locus_tag="VFQ61_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9953675.1"
                     /translation="MKYMLMMNTGCGAYQITSWPREDIQAHIGFMIQFAKKLAQSGEL
                     VAAEGLAGPDQAKSVRAGKDGKPVTDGVFPESKEFLAGYWIVDVESPERAYQIAAEAS
                     AAPGPKGVPLNMAIEVREVMSGPPKDFL"
     gene            13663..14991
                     /locus_tag="VFQ61_04200"
     CDS             13663..14991
                     /locus_tag="VFQ61_04200"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HET9953676.1"
                     /translation="MSRFKVKRLSRLHLRPFHHYALVWHRYVGLGLAALLVVVGLTGS
                     LLAFQHELDVALNPELWRAIPEPGARLLEPFELSERVQRQLPSGLSHGSVQFELVPGE
                     ARPFWVESAPGRWEQHFANPYTGELLGSRRWGDIAEGKRNLVPFLYRLHYALALDGFG
                     QWLLGIAALLWSLDCFVGAYLTFPVRSPGAGSRSPVGWLRRWFPAWLVRTSKPFAMVF
                     TGHRAAGLWLWGMLFVFAWSAVGLNLPHVYRPVTRAVFGLEPNVHDQLPQLAAPFPKP
                     GLSLQVAHARARELMAQAAEQRAFRIQREVSLQYLADHGAFMYRVQSTLDISATRPRT
                     EVYFDGRSGKPLGFNAALGPTAGNTLTSWLYALHFSEVGGLPYRLFVTCVGAVVAALS
                     ASGAWIWWVKRRKRTRARSLVREGTAAAPVLVNARARPHIDSGRLDQEEA"
     gene            complement(14988..16082)
                     /locus_tag="VFQ61_04205"
     CDS             complement(14988..16082)
                     /locus_tag="VFQ61_04205"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9953677.1"
                     /translation="MERSSEQPCSVCGLVPLIPRLKGATEIVARSSIMQGLLKRVARF
                     ASSEAPVAVLGETGTGKEVIARTLHANSKRSKQAFVAVNVAALPADLLESELFGHGRG
                     AFTGAANARRGLFEAAHQGTLFLDEIGEMPIALQAKLLRALQDGEVRRVGESEPFVVD
                     VRVLCATHRDLAARVREGTFREDLFYRLKVLTLQVPPLRERGEDVLPLAYQFLAKENT
                     RARGFARAAQQAMLAYGWPGNVRELENAVKHGAALATGNEVQLEDLPEELHSAQAAPS
                     TTRSAPDRSTPSASGSPAPPSSPGPNLPAPGSSRSGVVAQDQRSLAEVEREHILRVLD
                     ACGGSQSDAARILGVGRNTLWRKLRSYSEL"
     gene            16206..16958
                     /locus_tag="VFQ61_04210"
     CDS             16206..16958
                     /locus_tag="VFQ61_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HET9953678.1"
                     /translation="MEGNPMLSSLLDPERSVASLVLERSECADVFLRHQIDFCCRGNV
                     SLSVAAESRGLEVTQLLTELDHVISDRTRTFELNPATLDTARLIEHIVSKHHHYLRQV
                     LPSVQFLAQKVGRVHGERNPKLRTLDALVQELATALLPHLDEEEQSLFPLLCSSSSDG
                     GAIESELTAMLDDHLAVAKLLQATRAAADDFVPPDWACNSYHTLISELAHLERDVFTH
                     IHLENHVLRPRFLAEAAPIAAATLLAKSAARS"
     regulatory      16964..17024
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01763"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-III (ykkC-III) riboswitch; guanidine
                     riboswitches control genes whose products are involved in
                     modification or pumping out guanidine as a toxic compound
                     from bacteria; this is a third class of guanidine
                     riboswitches based on ykkC-III RNA motif; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF01763"
     gene            17126..17461
                     /locus_tag="VFQ61_04215"
     CDS             17126..17461
                     /locus_tag="VFQ61_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HET9953679.1"
                     /translation="MITRAWLYLITAGLLEIAWALGLKYSDGFTKPLASGLTLISLIG
                     SMWLLSLSVRDIPLGTAYAVWVGIGATGTALLGAALFSEAISPWRVLFLVLLVTSIIG
                     LKITAVEPH"
     gene            17498..18643
                     /locus_tag="VFQ61_04220"
     CDS             17498..18643
                     /locus_tag="VFQ61_04220"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HET9953680.1"
                     /translation="MRSVLIVLISLHGLIHVLGFLKAFELARLPQLRLPISTALGVLW
                     LSAAILILASMAAWLTWPRGWWLIGAVAVVSSQSAIVSSWSDAKFGTWPNLLLLGLVV
                     YGCATQGPGSFRARFDAESTASQAEDRTAQSEAPAPPLRLEERDLLALPPPVQRYLRA
                     TGFVGQPRVASYRLRFRGRIRGTPNDAWLSFDAEQRSVVQPATRLFLMSAQMKGLPVQ
                     AFHRFVAASASMQVKLAGLFSMVDQKGPELSRAETVTVFNDMCLLAPGALVSPAVQWV
                     DWDEHRAHARFTSGAHTIAATLEFDDDGLLRNFVSDDRSRTSADGRTLTRLRFSTPVR
                     DYKPYGPYRLASLGEARWHAPPPEGTFTYGEFELVEAQFDEAASSAR"
     gene            complement(18663..20297)
                     /gene="rtcR"
                     /locus_tag="VFQ61_04225"
     CDS             complement(18663..20297)
                     /gene="rtcR"
                     /locus_tag="VFQ61_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair transcriptional activator RtcR"
                     /protein_id="HET9953681.1"
                     /translation="MRPNPPKKLVVIGLLGTTLDQGKGPNRWQRWRPTVALCQHEDLL
                     VDRLELLVPQRCEALAQIVSSDIANVSPETLVSAHTFDLEDPWALEEVYERLYDFAAH
                     YPFDTEAEDYLVHITTGTHIAQICLFLLTESRHIPARLVQTSPPTRAFPSPAGGCVII
                     DLDLSKYDRIASRFRKEHHDALASLKSGIRTQNKAFNQLVERIERVATRSKDPVLLLG
                     PTGAGKSQLAKRIYELKRARRQIEGEFVEVNCATIRGDAAMSTLFGHLRGAFTGAANE
                     RSGMLKKAHGGLLFLDEVGELGLDEQAMLLRAIEEKTYYPMGSDREVRSEFQLVAGTN
                     RDLALAVAQGRFREDLLARVNLWTFRLPSLRERPEDFAPNLDYELEQCARVLGSRVRF
                     SREAKERFLAFAVSEEAKWSGNFRDFNAAVRRMATLCEGGRIKQTDVNEEIGRLREAW
                     RSVSEAELPQGAASAADLVSTVLGPERAELLDAFDRVQLAEVLRVCQESKTLSDAGRR
                     LFEVSRSHKRAPNDADRLRKYLARFGIEWSRLRPRG"
     gene            20466..21716
                     /locus_tag="VFQ61_04230"
     CDS             20466..21716
                     /locus_tag="VFQ61_04230"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HET9953682.1"
                     /translation="MDSCNQSRSYDVFSSTHGSPVKAWTRGVPFEESAKAQLQRIAEL
                     PFIHKWIAVMPDVHQGKGATVGSVIAARGAVIPAAVGVDIGCGMIAVRTSLSADDLPD
                     DLRALRSAIEAAVPHGRTDNGGSNDRGAWHDVPESCAHAWSTLRASYEQIVSKHPRIG
                     RGPSFTHLGTLGTGNHFIEMCLDDSSPSARVWFMLHSGSRGLGNRIGSYFIELAKQEM
                     RRWFINLPDADLAYLPEGTRYFQDYIESVAFAQEYARWNRELMMQAVVNAVRRTGLLP
                     PFEASLEAINCHHNYVAVEHHYGENVFLTRKGAVRARHGDLGIIPGSMGARSYIVRGL
                     GNPESFHSCSHGAGRVMSRTEAKRRFTIEQHAEATAGIECRKDADVIDETPAAYKSID
                     QVMQAQRDLVEIVYTLRQVLCVKG"
     gene            21808..22827
                     /gene="rtcA"
                     /locus_tag="VFQ61_04235"
     CDS             21808..22827
                     /gene="rtcA"
                     /locus_tag="VFQ61_04235"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HET9953683.1"
                     /translation="MLILDGSRGEGGGQILRTALALSLVTQQPFRITNIRAGRSKPGL
                     LRQHLTAVQAAAAVSGGTAEGAALGSRELTFHPGSVHAGDYRFAVGTAGSATLVFQTI
                     APALLRASQASTLLIEGGTHNPQAPPFESLNEAFLPCLRTMGFEVSAELERPGFYPAG
                     GGRFRVKIAPASALRPLRLLERGALKSRLASALVTGIGSQIAHRELRVIGQRLGWSRS
                     ELRCEPLDESYGPGNAVLIRIQSENITEVFAGFGQRRLRAEQVGEQVACEALEYLESG
                     AAVGPHLADQLLLPMALAGGGTFSSTSPSSHTLTQAETLRLFLGIETRIEAETDRRWR
                     LEVGA"
     gene            23001..23531
                     /locus_tag="VFQ61_04240"
     CDS             23001..23531
                     /locus_tag="VFQ61_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9953684.1"
                     /translation="MTKLVSIGRRTEVTTPPDLVALLLECHERIRTFAGIAVDVARRS
                     DLSSSDVAEACRRCIRYFEQALPLHVLDEERSLLPRLSQTNEEVRAALTAMHNQHHAH
                     EPQLVEFLAGLRDLEQDPLSGDARARLEPVAVELAAQFEDHLVLEERVIFPAIREHLS
                     VADQTEVLAELRARRQ"
     gene            23642..25054
                     /locus_tag="VFQ61_04245"
     CDS             23642..25054
                     /locus_tag="VFQ61_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET9953685.1"
                     /translation="MRNISGSVPSWIGASWFLLMTLVGCSSRADEPGSLPIAGQGGFT
                     NKPGTGGSTESNAGGAGSGGIASGGTTTIGGASMGGVPTGGVASAGSTAGGKAQGGSS
                     QSGGAAGSAGSGGKSAGFGGVGGVSNSGGNAGSGGRAGSGGNAGSATGGIGAGGIGAG
                     GIGAGGAAGSGGTVANTGGSTSSAGSTGQTRDILIPPSGAWLGMYYGDATLAATQQKL
                     GRSVPLHLVYYAWSDDFTRDATAEDLRAGRVPLINWELFDITLDEILSGSQDTLIQKR
                     AQALKQLAKPLFLDFGAEMNGDWSPWGGAQNGKSADKYIQVYRHVHDIFERVGASNVI
                     WAWCPNVTDVPREPWNEALDYYPGDKYVDWVCVDGYNWGDTNEGVWESFATVFKDIYP
                     KLASKNKPILIGEMASAEIGGNKAAWIDEMIPTLKDRFPAIRALVWFDIKKETDWRIG
                     SSTASLAAFTRLAKDPYFNP"
     gene            complement(25113..25997)
                     /locus_tag="VFQ61_04250"
     CDS             complement(25113..25997)
                     /locus_tag="VFQ61_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953686.1"
                     /translation="MSGASGCGPGEGSVGVAALPARPPTFEVQFANDDPSPLSSRASV
                     PGSVVQLSGEAAAASDGLAAQLRWPGHPEFGPADYSGVDSRTEITIVRNLGYGTYRSR
                     LAFGRCEPSEQVVHAMLGFYNDGADRNANGLRDDVEIDFQVLCGDPSLAYLTVFTDYE
                     VSPEGDELFRKLARVVDLATGRYYETPADDRDDFVLAGTDPALRLDGWFAPNQFYELG
                     FEWHAGILRFFVVLDSDPTERTLWTLADSSHVPEPPVQVIYNLWHPDSLWFPRDGDAD
                     FPQRDLTMQIDWFRYWAE"
     gene            complement(26086..27072)
                     /locus_tag="VFQ61_04255"
     CDS             complement(26086..27072)
                     /locus_tag="VFQ61_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953687.1"
                     /translation="MTETQKRLVELEPESLTAELIEAARHQQLSATERQELWSNIVAN
                     LPGPPAPPGGRASPHTEPSARPEPGPELPSPPASNLPAGAGLSIGKGLTWIVGATALG
                     IAGYATVRSAPRVIVSSPVAITAPSVNRGDSGAASSASNGAGSNVSTNGTAAQQDARG
                     HAPAAENESALDHPVPSASAGREQLVHAKKGTGVAELQADSVNSVPTSARSSRAGEMP
                     ASPKSARPAVSVEEPKAAATEASSLREESQLLLEARSSLRTGNASQALAILGRGRARF
                     PRGPLMQEREALYVEALARSGQKQAAAQSAELFLRNYPKSPHAADVRRYLSP"
     gene            complement(27069..27605)
                     /locus_tag="VFQ61_04260"
     CDS             complement(27069..27605)
                     /locus_tag="VFQ61_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9953688.1"
                     /translation="MNLQQIYEQHADFVWRTVLRLGVAEAEARDAVQDVFLRVHAHLG
                     EFEGRSSIKTWLFAIARSVARDRRRRARLAPGLAADGELEETVDLRADVGRVAEHHER
                     LLILERILDGMSADQRTVFVLFEVEHFSGEEISEALGAPIGTIYSRLHSARKLFRAAV
                     SRMQAQEHFLECRTGEAS"
     gene            complement(27672..28676)
                     /locus_tag="VFQ61_04265"
     CDS             complement(27672..28676)
                     /locus_tag="VFQ61_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953689.1"
                     /translation="MQGRAWTKIGGCTAVAGLGSILVLSPARAVQPLRLEVARSLQAA
                     DCPDSVDLERRISQVLGRPLAETRDAAETLRVSVDFDRRRGAFFAVLRFDGSKRGERN
                     LRDPSASCAALADAVTATLALVLDRVIERGEREPPLEPSSVSNQPNAGRKAVKSDNTA
                     GEQPRVPWGLALAAGAGVGVGFVPELGWMIGGGLQARGPLGLELGLSIQRMFAPELSL
                     AQGRVETSLTFGRVQICRFVGSTVRWAPCVQYGFGALRGAGHDYSENRSTSLFWSALG
                     PGLLGRLSLTRAIFVQVRAEAWIPLRRLSLTVENGGRPLQTPGIAGDLGLEFGVDWAL
                     "
     gene            complement(28724..>30222)
                     /locus_tag="VFQ61_04270"
     CDS             complement(28724..>30222)
                     /locus_tag="VFQ61_04270"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9953690.1"
                     /translation="PTDALGNWPDLLLNALDEELDGKEGRFNGLFEELLEEAQSEGAS
                     LDEFQRVVSVLRAEFQRTSRSEEDSSRLERIWHDARVLVGAASIRLLGRQKIDQQIAN
                     SRLGWVGERLATTLSLPLLKKEIFDGLAQLQISRGAVSLHSHELSGQLKTLVIKTDDG
                     EPGIEDGAFPETELAPRALFDTASCQHFVALPITFETEMLGLAVFAGDAQSSVYEPLR
                     QQIGSAIKGAMLHREMVAHVASRAQLEADRVAKEARIAAVIQTSINPVFTEIPGLEVA
                     ALMVPAAEAGGDYYDVIATARGAWIGIGDVTGHGLGAGLIMLMIQSMIAGLTRMQPAP
                     SPSQVVSTVGDALWDSVRLRLKRDDHATLTVLHYQGSGRFTFAGAHEDLIVWRSRTGR
                     CELVPTPGFWVGALPSVRHMTQDSELVLSPGDLLILHTDGITEARNTRHEQFSLERLV
                     RYIEELARQPAAVIRDRVQEAVSSWSASLDDDVTLLVVRYLGEGSAAG"
BASE COUNT         5582 a         9712 c         9479 g         5449 t
ORIGIN      
        1 gctcagaagc aggaaaattg caccgcggcg atgaagctgc ccgtctgcca atccctggct
       61 tcatttctga gccgttcagc gtccagggcg taaacgcccg aatctccgag gacccctcaa
      121 gcaagcggag cggcaactca agttgccctc gacagagtcg ccgattcgaa acgctcggcg
      181 cactcgccca gcgtcgcgtg cgggaaacca gactctcgcg actgctgaat ctagtctgtc
      241 gaaggcccta gttcgacgag gtcgctctaa gtggcgagcc acgcggtgag tggctcaacc
      301 tgcgcgcaga tttaatcggc ttgagcgata tcgacggcgc gcgttctacc tgcatcactc
      361 ttttggaacc tggcaggagc gacgcgcatc gcgtcgcaga atgagcgtcg agacacgcca
      421 ctcattccac gcgagaccat ctcaaaaatc gatcatcgtg tttgcgactg accttgcttt
      481 gagcatcaag gcgacggaat attggtgggc catcatgtcc tatggggttc gcctatctct
      541 ccttgcccta agcttcgcca cattggcttc ttcgagcgcg tgttcatcca acgaaggttc
      601 ttcgaaccgc ggtggtggtg gacggagcgg cggaggagca gctggaaccg gtctcagccc
      661 cggtactggc aactcaccgg gtactggcaa ctcgccagga accataccta tccctagacc
      721 ccctggcggg aactgcggca atggcaagca ggatagcgac gagctgtgtg acgacgccaa
      781 caaggaagcc ggtgacggtt gcaatccggc ttgtcgcgtc gaggcggact tcgagtgccc
      841 cacctttggg cagccgtgtg tgagcaaagc catctgcgga gatggcaagc tcgcgagcac
      901 cgaaacctgt gatgacatga acaccgtggc cggggacggc tgctcggcaa attgccaagc
      961 ggtggagccg ggttggcagt gccgcgtgcc aggtcaacat tgcattccgc tctgtggcga
     1021 tagcgtcatc acggcccccg agaactgcga tgatgccaat gcgaccagcg gagatggctg
     1081 ttcgagcacg tgtcttacgg agccgggcta tgactgcaag gcgcctggcc aaccctgcgt
     1141 aaagtccgtt tgcggcaacg gcgtgaccga agccggcgaa acctgcgacg cgggcatgaa
     1201 taacggcttg ttcttcggcg acggcaaggg ttgctccaag acgtgcacgc tggagcccac
     1261 ctgtcgcgac gacaaaggcg tgaccacggc ctgcaagacg ccctgtggtg atggcaataa
     1321 ggacccgggc gaggaatgcg atgacggcaa cgggtcgaat ggcgacggtt gctcgaagga
     1381 gtgcaaggtc gaagtcgacg cgggctttgc gtgcgaagac aaggtgatca aggacactca
     1441 gccgtgttcg gatggcgcgg gcgagtgctt ggtgctgccg atcgtgtatc gcgacttcga
     1501 cgggcagaat caaccgacgg ggcacccgga ctttttctat ctgtcgtcgg gcaccaccaa
     1561 gtgcgtcccg aacgccagcg gaaataagga cacgaccact actgccgggg attgttggaa
     1621 gagtgactcg acggatctgt gctcagggct ggtcgaagcc acccttggtg cggatggcaa
     1681 gcccgtttac agcgcaacca aggctggtac ttgccagtgc cgctttacgg actggaatca
     1741 gacgaccgtt gttcccgctg gcaccattcc cgctggcacc gcttgcaccg tgcgggacaa
     1801 caacggtggt gcgcaaggta ctaggaaccg gatcgaggca aacgttcagg ctatccagag
     1861 tgcagcaagc ttcaaggact ggtattcgga ttcggcgaag agcaccaagg tcacggacaa
     1921 gctggagctc aagttgctcg ccggaaatca gtatcaattc agcagcagca acggacgcac
     1981 cgtctacgag gatattcacg atattttcct cgcaagtaag ggagaaccgg cattgccggg
     2041 tgcggtcact agcttgacga gcggcttctt tcctcttgaa gcgcagacgc gcccgaaagt
     2101 ctgcaacatt tggccctatt gggtcgcaga tggccaaagc agctgcgcca gcggagccac
     2161 cctggacgtc cgcaatcagt gggatcccaa gggctggtac gagggcacca aacccgccaa
     2221 tccgatgggc ggggaggtcg cgagtgtcac gggcgtgaag cgcaacttct acttcacctc
     2281 ggaagtgcgt tacctcttcc gttatgcagg cggcgaggtg ctctcgttct acggcgatga
     2341 cgacgtttgg gtgttcatca acggcaagtt ggcgctggac ttgggggcgc cgcacgagcg
     2401 cctccaaggc tccgtcactt tgaccccgac cggcgccaac tggacgatca acaccgccgc
     2461 taagaacgtt accggcaccg tccaactcgg caccttcgag caaggcaaga cttacgaaat
     2521 cgtggtgttc cacgccgacc gccacccgcg tgagtcgaat taccaactca cgctgtcggg
     2581 cttcagcacc acccagtcgg tctgcaaggc gaactgcggt gacggcaagg tcgcggccgg
     2641 cgaagagtgc gacaacggcg cgatgaacca ggacggcctg tatggcggct gcaccacgca
     2701 gtgcaagttc ggtccgttct gtggcgataa ggaagtcaac ggcaccgagg agtgcgactt
     2761 gggcaaggac aacggcgccc agtacggaca agagggttgc accaaggcct gcacgctgcc
     2821 ccaccgctgc ggtgacggca tcatcgacag caagttcaat gaggcttgcg acgatggtcc
     2881 gaacaacggc aagggaccgt gcaaccccga ttgcaccttc gtcccactgt agacctcgtc
     2941 atcccggaga cccgggctcg ctagcgcgag tctaggttaa gcgcggccct tccaggcttc
     3001 ctgggagggc cgtcgcgttt tgtgctgcat ttatcgagcg tcgccgagta aatcatggca
     3061 agccgcttgc cgcgcaccat caaggcccca cgagctaacc gccatgaaga actcgggtcg
     3121 cgggcgcggc cctgaccatc aaaaacccgt cgaaccgact ccggccgagc ttggaaccga
     3181 ggcggcctcg ccagagcgcg ggcgcgatgc agcgctcacc caccgcacgg tggacgcgac
     3241 tttggcagcc gagtggctct ggggttggga cgctaccccg ggcatcgtgt cggtctgggc
     3301 ggagatggat gggcgtgtct gggtttggcg gcggagcccg gaaaccttgg agctcgtgca
     3361 ggaacacgtg cgtttccgtc cgtggttact gctcggttca ctccgcgatc tcgaacatct
     3421 gcaaaaccgg ctcgtcccct cctcttccaa gcacgctcgc gagcgcggcc acttcagcta
     3481 tcacgagctg aacggcccgg gtgagctgcg gtattgggta tccgccgcag acgggcgtga
     3541 cctcacgcgc gcggtgctgc ggggtgcctc cgagcggctc ggctatgaag tccgggacgt
     3601 gcgcgagctc gcaccggacg tggtcctgct gtcgcccgaa gagcagtatc tcacgcgcag
     3661 cggccgcact tacttccgcg gtctgtcctt cgatcaagtg cgccgcatgc acctcgatct
     3721 cgagaccacc ggtctcgacg ccgaagtcga tcgcattttc ctggtggcgc tgcgaatgcc
     3781 ggacggccgc gccgaggtgc tccgcggcga aagccacgac gacgtcggcg aagccgcatt
     3841 gttgcgcaaa acgcttgctc gcatcgccga atacgacccg gacgtgctcg agaaccacaa
     3901 cctgcacggc ttcgacgtcc cgttcttggt gcggcggtgc cagcacctcg ggattaagcc
     3961 ggacttaggt cgtttaccgg gactgaagaa gcgcgccgcc gcacgcggca tggtcaacgc
     4021 tttgtcgacg gcggcgggcc cacaagcccg accgcgtgat tcggaagaaa tgctcagccg
     4081 ctctccaacc agcctatctc gacgcgcgcg cttcagcgtg ccggggcgag agctgctcga
     4141 tacattggat gcggttctac gctacgattt cgcggctcgt gatttgccgg ggcacggctt
     4201 gaaagaggtg gcgcgtcacc tcgggctggc tcaaagcgac cgcgagtatt tgccagcggc
     4261 ccaggccttc tctctgttcc agcacgatcc agagcgggtg atgcgctacg cacgcgatga
     4321 tgtgcaagaa gccgcggccg tcggtcgtct gttgggcggg gctgcgtttg ggctcgcgca
     4381 aatggcgccc cgccgttatg aacgcttggc cgacgcaggc cccgcgacgg gggtgctgga
     4441 tccgatgttg ttgcgcgcgt acctgcactc ggacacggcg ttgccgcgcc cgtcaggcgg
     4501 agatgggacc ccgcacacgg gggccgcgct ttatctcttc gccacgggag ttgcccaaaa
     4561 catcgtcaag gctgacgttg cgagtctgta tccgtcgctg atgcggcagt accgaattgg
     4621 gccacaaact gacgtattgg gcgcgctact caccttggtg gagcattggg tcgagcaacg
     4681 gctacgtgcc aaatcactgg ctaaaacgtg cccgcctcat tccccggagc gctttggtca
     4741 cgaggctttg gcagccgcgc taaagctgct cgtgaactcg gcgtacggct acctcggcgc
     4801 ggtcgggctc acgcgttttt ccgacgtgca cgcggccaac gaggtgacgc gccacgggcg
     4861 cgagctcttg gatctcttgt gccaggagct cgcggagcgc ggggtcaccc tgctcgaggc
     4921 tgatacggac ggcgtttact tttcggtgcc gcccggcacg accgaagcgc aagaacgagg
     4981 catcgtggag caggtggctg cgctgctacc cgagcaagtt cacttggagt tcgacgggcg
     5041 ctacaagacg atgctctcgc acgagcccaa gaactacgcg ctgctgggct acgacgacca
     5101 gctgatcttg cgcggcgtgg cttttcgctc gcgacgcgcg gagccgttcg gggagctctt
     5161 cctggagcgt gctttgcgcg ccttgttgtc gggagatatc gagggcgtgc ggcagtcctt
     5221 catcgatacg ttacgcgcgc tgcggcgtcg tgagcttccg accggcgcgg tcgccgcgct
     5281 ggttcgcctg accaagacgc ccgagcaata cctcgcggcg cgcgagaagc gccgtgagct
     5341 gccatacgag gcgctgatcg gtcgggggcg ttttcagtgg agcgttggcg agcggatccg
     5401 cgtgtaccgg acccagggcg gtggggcggg actgttcgat gaggaggcga gcgaggacgc
     5461 gcgtgactac cacgtcgtct actacgctcg tctcttgaga gatacttttg cggcgcggct
     5521 ggcgcgtgcg ttcagtcgcg aagactacga gctgttgttt gctgatccga cccagctctc
     5581 cttattcgag aggccgatcc gcagcgttcg tccgatcctt acgaccggcg tatcctcgct
     5641 gccggcaact cgcgctcgat aagaatctta ggcctcggtc gagcactccg cgtcgtccgc
     5701 ggtgggtgct gcaccggggg cgtgcttcct ggccagcgcg acggctccta cagccaggcc
     5761 gagaatcccc gccaagccgc tggcgcatag aattccgagc ttcgccgcac cgagcaggcc
     5821 tgcgtcatcg aacgcaagct gcgccacgaa cagcgccatc gtgaagccaa tcccggccac
     5881 catcccgagc accactaggt gagccttgcc aagcccgcgc ggtaacgtag ccagcccgag
     5941 cttcaaaacc agcgcgcaag cgatcagaat tcccaccggt tttcctacga tcagacccgc
     6001 ggcagcgccc agtaacaccg cggtctcgtg ttccccgaat cgtacgccgg agagtgtgac
     6061 tcccgcgttg gccagggcga acaacggcat tatgatgaac gcgacccagg gatggagcgt
     6121 ctcgatcaag ctctcggcag gtgaacgcgc ttcgcgatgt gcaacgttga cgtcacgcag
     6181 gacctctgaa agctgcccgg ggctaaccgc agagacgtcg cgctcggccg ccaaaggttc
     6241 gatcttttcc tgcaaaccgc gcacgaaacc ttccgccccg taccaagctc gcaccggcgt
     6301 catcaggcca atcgcgaccc ccgcgatcgt cgggtggatt ccggcggcgt aggtgccccc
     6361 ccacgcgacc aaaccgggtg cgatgtacgc gagcttgttg cgaaccccga gccactgaag
     6421 aagcaggatc gaaagcaatc cgagcgcagc caccgccaag cccacccact gcactcccga
     6481 ggaatagaac aaggcaatca ctaggattgc ccccaggtca tcgatcacgg ccaacgccaa
     6541 tagcaacaca cgcaacgcgg gtggcacccg ttggcccaac aaggtcaaca cgcccagggc
     6601 gaaggcaatg tccgtcgcca tgggcactcc ccaaccagag tgcgtcttgg gcgcaccagc
     6661 tatgaggaga tagatgccgg ccggcgccaa catgccgccc agagccgctg cggcaggcaa
     6721 cgcggctcgc cgccactcag acagctcgcc ttggtgaatt tcgcggcgga tttccagacc
     6781 gaccacgaag aagaagatca ccatcagccc atcgtttacg acccactcca ggctccgttc
     6841 gaagctgaag ccgccgatgc gcacgccgag cggtgttcga aacaattcgt gatagccgtg
     6901 ctgccagctt gaattcgccc acagcagcgc caccgcggcg gccaagagca gcaagattcc
     6961 gctcgccgct tcgatgtgca ggaagcgctc gagcggtcgt gttgccaata gccctagacg
     7021 gcgaattggg ctccacgctt cgggaggact ggggctggga gaacgattga gtcgatctgt
     7081 cgattgcatg acctaatccc tcggacaatt tccgaagagg cggcggcccg accgactcac
     7141 tcctgcggct ctcgccgcac cctttgaatt tgcggctctc gccgcatcct ttgagatttg
     7201 cggctctcgc cgccgtcctg actattcccg cggttcgcgc cgtacctttt gagatttgcg
     7261 gcgctcgccg ccgtcgtgac tattcccgcg gctcgcgccg tgaagcttgg gtatttggat
     7321 tgtaaggatg ctgtgtatcg tgagcccgcg ggcagcggcc cgcgcagcga aagttctatg
     7381 tttcacgaag ggtttgatgg tgcaagagtc aaccggcgtg ccgcctgcaa aaaggtcccc
     7441 gcaccgaagc tcgagacgtg ctccaatagt gcgtcttcat gacttcgccg caaagccgcg
     7501 atgtggtcac gcccgagggg tgccaagctc aagggcaagc tccaggtatt gcccctgaag
     7561 gtacagcagc tccggctagc acccctggca gcagagccgc tgccggcaaa acaacggagc
     7621 actctgacgt gctcgcgccg gaagcacgcc gcgcgctgct cgacgcccca cgtccccgct
     7681 cattgcccat cgcgcccaat gccccgcgcg ccctgcgcca cctccctctg gcggagtctg
     7741 cgcgtgacgt tgaccgcgag gcacgcccca tttacgcggt gtgggagatc accttgcgtt
     7801 gcgatttggc gtgtcggcac tgcggctcac gcgccggtca cgcgcgcagc gacgagctga
     7861 gtaccagcga atgcctcgat ctcgtcgctc agatggcgga cctcggcgtg ctcgaggtga
     7921 ccctgatcgg cggagaagcg tacctgaggg acgattggac ggagatcgtt tcggccatca
     7981 aagcacgcgg tctatccgta acgatgacca ccggggggcg cggcatcacc gcggagcgtg
     8041 ctgaacaggc gagccgagct gggctcgaga gcgctagcat ctcgctcgat ggcttggccg
     8101 agactcacga tcggctccga ggagtgcgcg gttctttcga gagcgccctg caagcgatgg
     8161 atcatctgcg tcgggccggc gtgaaggtgt cctgcaatag ccagatcaat cgcctcagtt
     8221 tgccggagct cgcgccgctg ctcgagctca tcgccgaacg cggcgcgcac agctggcaat
     8281 gggcgatgac cgtacccatg ggccgcgctg cagacgagcc cgatgtgctc ttgcagcctt
     8341 acgagctgct cgaagtcttc ccggagcttg cgcgcgtgca tcgccgcgcg gaggagctct
     8401 cggtcaagat atggcccggc aacaacgtgg gttacttcgg cccctacgag cacgaactac
     8461 gccgctactc gccgcgcggt cacgcgagtt cctgcgccgc gggtcggctc gtgctcggaa
     8521 tcgaggcgga cggtagcatc aagggctgcc cgtctttgcc caccgcagct tggacgggcg
     8581 gcaacatccg tgaccaatcc ctgcgggaca tctgggagcg ttcgaagccg ctccgttata
     8641 cccgtgatcg aacagtcgag gatctctggg gcttctgccg gtcctgttat tacgcggacg
     8701 agtgtcgagc cggctgcacc tggacttctt tcgtgttctt cggacgcgcc ggcaacaacc
     8761 cttattgtca ccaccgcgcg ctcgagatgc agcgccaagg gttgcgcgag cgcgtgcgac
     8821 gtatacaggc cgcccccggc gagcccttcg atcacggaaa cttcgaaata gtagtagagc
     8881 caaacccctc tttggagccc tcagcatgac tcgactattc gcctgcccta gctgccaccg
     8941 acacgtgaag gtggacgaaa cccagtgccc gttttgctcg acggcattct cgtcgacaga
     9001 gcaccccgag cgccctgtgc cgacgcaacg catgtcgcgc gccgccagtt tcgcgttcag
     9061 cgcgacggtg gcggccggca cttttgcggc tgcgtgcagc ggtcaaaccg agggaacccc
     9121 cgtgcccgtc tatggggctc ccgcgggagg agtgggtgcc atgacctcca ccggaggcag
     9181 cagtggagcg ggcggacagg ccggaggttc tgcgacgagt ggtgggaacc cctccagcgg
     9241 cggtgcgacc aacacgggtg ggcggacgag cagcggtggt caggtctcta ccggcggagc
     9301 atcgatggcc accggtggcc gaacgggaag cggaggcaag gcctctggag gtgccggacc
     9361 cgatccgttc gggggcgcca ttgcggtgta cggcgcgccg cctcctacgg gcggacgctc
     9421 caacgcgagc ggcggcgcga acggtgaagc gggctcggtc ggcgaggccg gtgccgcggg
     9481 tgacgtcggc acggcgggcg caactgatcc aggtactctg ccagtcccgc tctatggcgc
     9541 gccggcaaac ctgaagtgag ccgcggggca cgaaacgtag agagccccgc gaactcaaac
     9601 ggaatcgcgg tccgaatcaa gaccagtaac caaggaggcg aagtgagagc actcgtgcgc
     9661 gcagcccgct tcgcctctac tgacgtgcat gatccctggg ggtgattgga cctcacccgc
     9721 caagaagggc ccagtcagct ctcgagcgac gcgcggagga accaggcgtg cttctcgaac
     9781 tcggtcacga tttgcgtgaa caagtcgacc gtgtcggtgt cgccgagctt ctccgcatta
     9841 cttcggcttt cacgcaccac gtccaagtat ttctcgatgc gctctgcgag caaacgcacg
     9901 tggtccatgt cccgcgtggt gtcctgcggg tattccggaa gctgagactg cttggcgacg
     9961 tgacgggccg tgccatacgc ttttccgccc aaagtgacgg accgctcagc gatcgtgtcg
    10021 ttgtgagtgg ccaagctgac cgcgaaggtt tcgaacaaag ggtgcagcgc cgcaaactgc
    10081 gggcccttga tgttccaatg cgcgactttg atttggctgt gcagatcgag gccgtcagct
    10141 agccgagcat tcagggtttc acacaaactc acgcgcgcct gttccggaag gtggctaggg
    10201 ctcttgtaca tctttgggtt ctcctggcgt ggaatgtcgc gccctcggat gcagatgtcc
    10261 agccccctcc ccgtggcgtt ttccgacgcg ccgtcgcgcg tccaggtcct gctgtcgctc
    10321 gtatcgtcag gcttttgggt cgacttacgt ctcgagtgtt gcagctcccg caaaggcaga
    10381 ggccccgaca cctgagctcc gaccttggaa aacaaaacac gttgagcggg atagatgctg
    10441 cggtgaccgg tcattaggta agcgagcacg ttgacgatca tcacgtgggg gaaaatacgc
    10501 gctccgagta gctccagagc cattatcgcg agcgcaatgg gtgtattgga ggcggcggcg
    10561 aacaccgagg ccaaccccac acccgcggcc aagtctagcg gtagccccag cccacgcgcg
    10621 agcgcgttgc ccaaggtggc accgatgaag aacaacggcg tcacctcgcc tcccaagaag
    10681 cccgtgccca acgtgaccgc agtgaacacc agcttcagaa accaagtgga tagaggcaag
    10741 gatccgtcca cgaacgctcg ctgaatcacc ggaaccccga gccccaagaa gtcatcggta
    10801 cccgaagcct gccacaacgc gatgaccgcg accccgccga cggccatgcg ccagcccaaa
    10861 cgcggcaagc gccgctcgct ctgctgcttg agccaatgag tcagctcgat gaacaacgtc
    10921 gtagtcagcg cgacggcggc ggcaaacagg atccacttca gaaacaacgc cggactcagc
    10981 tgcgtttcgg ccagcaccgg aaacgccgta tgttcgatgc cgagcgcacg tgtcgtgaag
    11041 tcgccgatca atgccgccac cagcgctggc caaagcgcgt ggtattcgat gcgtccgagc
    11101 agcacgaatt cgagcccgaa cacggtgccc gcgatgggcg ttccgaacac cgatccgaag
    11161 ccacccgcca ccccggccgc cagcagttga cgacgcaaga ccttcgtcga tttgagacga
    11221 tggcttagcc aatccgccag gctggcgccc atctgtacgg cagttccctc ccgaccggcg
    11281 ctgcctccga acaaatgggt gagcacggtt cctgcgagaa ccattgggcc catccgcaac
    11341 ggcaactcgg gaccagcgtc gtgcagacga tcaatcacca ggttgttgcc agcccgaatc
    11401 ggttgaccgt accgctcgta aaaccagccc agcgtcagcc ctgccaccgg taatgcccag
    11461 accagcccat ggtgccccgt gcgatactga gtggcccgct cgagcagcca cagaaatagc
    11521 gcagacgcga cgcccgcgaa caagccaacc gccacgccga gcgtagtcca ctgcacgagt
    11581 agaccgagcg gatgctgttc gcgctcggcg gctcggtccg ggtcgctcat tcacgctgag
    11641 ctatcaccaa acttcgggaa cgtcgacgtg aagcggagcg cgagaactcg accccggagc
    11701 accggaagct ggtacgtcag ctctcgatgc gccccgcccg ctcaagcaga tattcttgct
    11761 ccacgcgact cgtggtgcgc gccgcggccc tccggtaaag cgcggccgcg cccctcggat
    11821 ctcccgtgcg ctcgagcaag tgggcgcgca ccgcatcgag ccggtagtgc tcacgcaagc
    11881 gtggatcggt tcccaccttt tcgagacgtt cgagacccgc ggccgctccg tgaaccatgg
    11941 cgagtgcgat ggcgtgattg agcgccacca tcgggttgtc cgaaatctcc aagagcaccg
    12001 agtaaagcgc cagaatttgg ggccagtcgg tgtcttcggc ccgggccgcc tcgtcgtgca
    12061 gagccgcgat cgcggccaag agctggtacg gaccggtcgc gcctcgcggc aacgtgcgag
    12121 tcagcagctc cgtgccctcg gcaatgcggt tctggtccca aaggctacga tcctgctcat
    12181 ccaacgggat tagttctccg cgggggcccg accgcgccgg ccggcgggcg tccgtcaaca
    12241 gcatgagcgc taggaggccc gctacctcgg gttcgtcggg agccaaactg agcaacaggc
    12301 gtgtcacgcg gatggcctcc tgggaaagat cggttcgatg cagctcgtcc cccgagctcg
    12361 cggtgtagcc ctcgttgaag atgagataga gcacgtgagc cacggcctgc atgcgcgccg
    12421 cgcgcccttg ctccgtcggg cgttcgaacg gcacgcccga gctctggatg ctctgcttgg
    12481 cccgactgat tcgttgagcc atcgtcccct ccggcaccaa gaacgcgcgt gcgatctccg
    12541 gcgtggtcag ccccccgata gccctcaatg tgagcgcgat cgcggaggct tccgtgaggc
    12601 acgggtgaca gcacatgaac agcaggtcca aagtatcgtc ctcactggtg ccgaacagct
    12661 cgtccggcgc gatcgcgatc tgttcgtcgg gcgggatcag gctgacgact agtgcctccc
    12721 gatgcttgcg tgcgctgtcc gcgcgcacct gatcggtgag tcgccgcatg gcgactcgaa
    12781 cgagccaggc gcgcggattt tcgggcgtgc cctcacgggg ccactgggtg gccgccgcga
    12841 ggagcgcctc ctgcaccgcg tcctcggcgg cgctgaaatc tccgcaccgc cgaagcacac
    12901 gactcagcac ctgcggcgcg agctcacgca gcaagtgctg aaccgactcg atcaactcag
    12961 acgtcacggc ctgcacgtga ggtggggctc atgcgtttgg gaagaaccca gggcgtcaga
    13021 gaaaatcctt cggcggaccg ctcatcactt cgcgcacctc gatggccatg ttcagcggca
    13081 cgcccttggg gcccggagcg gccgatgctt cggcggcaat ttgataggct cgttctgggc
    13141 tctcgacatc cacgatccaa tagcccgcca agaactcctt ggactcgggg aacacgccgt
    13201 cggtaacggg cttaccgtct ttgccggcgc gcactgactt ggcttggtct ggcccggcca
    13261 gcccttcggc tgcaacgagc tcccctgatt gcgccaactt cttcgcaaac tgaatcatga
    13321 acccgatgtg cgcctggatg tcctcacgtg gccagctggt gatttggtac gcgccgcatc
    13381 cggtgttcat catgagcatg tatttcatcg ttcgactccg tgtccgtgcg gctcgaccca
    13441 tcgatggcca gcgccgcgtg tttacccctg agtcggagcc gatgcccggc gctctacatg
    13501 ccccgtgtct ttttttcgaa gaaccaagga gcgcctctcc gaattccgca ctggacgaat
    13561 tcgcgaatca attgcaatat cgcccccaca ccagaggcgc ctcgcgcggc gctcccctgc
    13621 aactgtgttc cacccacaat ccgtatccct ccggtcaccg aaatgagccg cttcaaagtc
    13681 aaacggcttt ccaggctcca tttgagaccc tttcaccact atgccttggt gtggcaccgc
    13741 tatgtggggc tcgggttagc ggcgcttctg gtggtggtcg ggctcacggg gagcctgttg
    13801 gctttccaac acgaactcga cgtggctctg aacccggagc tgtggcgggc tatccctgag
    13861 cccggcgcgc ggttgctcga gcctttcgaa ctgagcgaac gggtccaacg gcaactgccc
    13921 tccggcctgt ctcacggcag cgtgcagttc gaactcgtgc ccggagaggc gcgccccttc
    13981 tgggtagaaa gcgcgcccgg acgctgggag cagcactttg ccaatccgta cacgggcgag
    14041 ctgctcggtt cgcggcgctg gggcgacatt gcagaaggca agcgcaacct agttccgttc
    14101 ttgtatcgat tgcattacgc gctcgcgctc gatgggttcg gacagtggct gcttggaatc
    14161 gcggcgttgc tatggagctt ggactgcttc gttggagctt acctcacctt cccggtacgt
    14221 agcccagggg cgggctcacg ctcgcccgtc ggttggttgc gccgctggtt tccagcgtgg
    14281 ctcgtccgta cgtccaagcc attcgcaatg gtgttcacgg gacaccgagc ggctggtctt
    14341 tggctctggg gcatgttgtt cgtgttcgcg tggtccgcgg tcgggctcaa cctgccgcac
    14401 gtctatcgac ccgtcactcg agcggtgttc gggctggaac ccaacgtgca cgatcagctg
    14461 ccgcagctgg ctgcgccttt cccgaaacca gggctaagct tgcaagtcgc ccacgcgcgc
    14521 gctcgcgagc tgatggccca ggcggccgaa cagcgcgcgt ttcgaattca gcgcgaggtc
    14581 tcgttgcagt acttggccga tcacggcgcg ttcatgtacc gcgtccaaag cacgctcgat
    14641 atctcggcga cgcgaccccg gacggaggtc tacttcgatg ggcgcagtgg caagccgcta
    14701 ggcttcaacg ccgcgctcgg accaaccgcc gggaacacgt tgacgagctg gttgtatgcc
    14761 ttgcacttct ccgaagtggg cgggttgcct taccggctat tcgtgacgtg cgtgggcgcg
    14821 gtggttgctg cgttgagcgc ttccggagct tggatttggt gggtcaagcg cagaaagcga
    14881 acccgagctc gctcactggt tcgggagggc acagcggccg caccggtatt agtgaacgcg
    14941 cgcgcgcggc ctcatatcga cagcggtcga ctcgaccagg aagaggccta aagttcgcta
    15001 taggagcgca gcttgcgcca cagcgtgttg cgccccactc caaggatgcg agccgcgtcc
    15061 gactgcgatc ctccacaagc atcgagcacg cgtaaaatgt gctctctctc cacctcggcc
    15121 agggatcgtt gatcttgcgc gaccacacct gatcgactcg aacccggcgc gggcagattg
    15181 ggcccgggag agctgggcgg cgctggggaa cccgaagccg aaggggttga acggtcaggc
    15241 gccgagcgcg tcgtcgaagg cgcggcttgc gccgagtgca actcctcggg aagatcctcg
    15301 agctgcacct cgttgccggt ggcgagggcg gctccgtgct tcaccgcgtt ctccagctcc
    15361 cgcacgttgc ctggccaacc atacgccagc attgcctgct gcgcggcccg agcaaagccg
    15421 cgagcacgcg tgttctcctt cgcaagaaac tgatacgcga gcggcaacac gtcttcaccc
    15481 cgctcccgca acggcggcac ctgcaatgtg agcaccttca gacgatagaa caggtcttct
    15541 cgaaaggtgc cctcacgaac ccgcgccgcc aaatctcggt gggtggcgca caacacgcgc
    15601 acgtccacca cgaagggctc actctccccg acccgacgca cttcaccgtc ttgtagcgca
    15661 cgcagcagct tggcctgcaa ggcaatcggc atctcgccaa tttcgtcgag gaacaacgtg
    15721 ccttggtggg ccgcctcgaa caacccgcgg cgcgcgttcg ctgcccccgt gaacgcccca
    15781 cgcccgtgac cgaacaactc ggactccaga agatctgcgg gtagtgcagc cacgttgaca
    15841 gccacgaagg cctgtttgga gcgcttcgag ttggcatgca gcgtccgcgc gatcacctcc
    15901 ttgcccgtcc cggtttcacc gagcaccgcg accggcgctt cgctcgaggc aaagcgagcc
    15961 acgcgcttga gcaacccttg catgatggaa ctgcgtgcga cgatctcggt cgcgcccttc
    16021 aggcgcggaa tcaacgggac caaaccgcag acgctgcacg gctgctcgga tgaacgctcc
    16081 acccgttcca ctctggaacg aggccatgcg cctgtccagc cggaactacc accaaagtga
    16141 cgcctatttg cgaggatttt gggtcttttc agggcgtggc gcgcgccctg caaaggccat
    16201 ccgccatgga aggtaacccc atgctgagta gcttgttgga ccccgaacgt tccgttgcga
    16261 gcttggtcct ggaacggtcc gagtgtgcgg acgtgttcct tcgccaccag atcgacttct
    16321 gctgccgagg gaacgtgagc ttgagtgtgg ccgccgaaag ccgaggcctg gaggtgaccc
    16381 aactgctgac cgagctcgat cacgtgatct cggacagaac ccggacgttc gagctcaatc
    16441 cggcgaccct ggacacggcc cgtttgatcg agcacatcgt ctccaagcac catcactacc
    16501 tccgacaggt gctgccttcg gtacaattcc tcgcgcagaa ggtcggacgt gttcacggag
    16561 agcgcaaccc caagctgcgc accctggacg cgctcgttca agaactagcc acggcgttgc
    16621 tgccacacct ggatgaagag gagcagtcgc tgttcccctt gttgtgctcg agctcgagtg
    16681 atgggggcgc gatcgagtcg gagctcacgg ccatgctgga cgatcacttg gccgtggcca
    16741 aactgcttca agccacccgc gccgcagcgg acgacttcgt ccctccggac tgggcttgta
    16801 acagctatca cacgcttatc tccgagcttg cacacctcga acgagacgta ttcacccaca
    16861 ttcatctaga aaatcacgta ctgcggcccc gcttcttggc tgaggccgcc ccgattgcgg
    16921 cggcgacgct acttgcgaag agcgcagccc gcagctaaga atcgcgcggc ctagctggct
    16981 cccgacgaga cgcgccgtac cgggtcacgc aaagacgacg ccgccactcg gcaagaacgc
    17041 acctgccgaa ggccatcttc gccattgcgc gtccgccgtg ggtaccgctc gggtgctcac
    17101 ggtcgacacg ggagatcgat cgagaatgat cacgcgtgcc tggctgtacc tcatcaccgc
    17161 aggtttgctg gaaatagctt gggctctggg gctcaaatac tctgacggct tcaccaaacc
    17221 cttggcgtcc gggctgacgc tgatcagctt gattggcagt atgtggctcc tgagcttatc
    17281 cgtacgggac ataccgctcg ggactgctta cgctgtgtgg gtcggcatcg gagccactgg
    17341 caccgcactg ctcggggcag cgctgttcag cgaagccatt tccccctggc gcgtgttgtt
    17401 tctggtgctg ttggtgactt cgatcattgg cctgaagatc acggcggtcg agccgcactg
    17461 atgttccccg acggcctcat tcagacgcag gtcgcagatg cgctcggttc tgattgtgct
    17521 catctccctt catggtctga tccatgttct gggatttttg aaggcgttcg aactggcacg
    17581 cctgcctcag ctgcgattgc cgatctcgac tgcgctaggg gtgctctggc tgtccgccgc
    17641 gatactcatt ctggcgtcga tggccgcctg gctgacatgg ccgcgcggct ggtggctcat
    17701 cggagcagtc gcagtggtat cgtcgcagtc ggccattgtc agttcgtggt cggatgcgaa
    17761 gttcggcacg tggccgaatc tgctcctgct ggggctggtg gtatacggct gtgcaacaca
    17821 gggtccaggc agctttcgag ctcgcttcga cgcggaaagc acggcatcgc aggctgaaga
    17881 tcgaacggct caatccgagg cgccggcgcc ccctctacgt ctggaggaac gcgacctcct
    17941 cgcgttaccg cccccggttc agcgttacct gcgcgcgacg gggttcgttg ggcagccccg
    18001 agttgcgagc taccgcttgc ggttccgggg gcgcattcgc gggacgccta acgacgcttg
    18061 gttgagcttc gacgctgaac aacgaagcgt cgtgcaaccc gcgacccgac tcttcctcat
    18121 gagcgcgcag atgaagggat tgccggtgca ggcatttcac cgcttcgttg cggcatccgc
    18181 gagtatgcag gtgaaactgg cagggctgtt ctcgatggtc gatcagaagg ggcccgagct
    18241 ctcgcgggcg gagacggtca cggtgttcaa cgatatgtgc ctgctggcgc cgggggcctt
    18301 ggtctcccct gctgtccaat gggtcgactg ggacgaacat cgagcccacg cccgatttac
    18361 gagcggggcg cataccatcg ccgcaacgct cgagttcgat gacgacggac tgctgcggaa
    18421 cttcgtgtcg gacgaccgct cgcgaacctc ggctgatggc cgtacgctca cacgcctccg
    18481 attctcgact cccgtgcgtg actataagcc gtacggccca tatcgcctgg cttcattggg
    18541 cgaggcacgc tggcatgcac caccgcccga aggcaccttc acttatggtg agttcgagct
    18601 ggtcgaggct cagttcgacg aggctgcttc gtcagcgcgg tgattcgatt ggatgggcgt
    18661 actcagcccc gcggcctgag ccgagaccac tcgatcccaa aacgcgccaa gtacttacgc
    18721 agccgatccg cgtcgttcgg cgctcgtttg tggctgcgag agacctcgaa caagcgccta
    18781 cccgcgtccg agagcgtctt ggactcctga cagacccgca ggacctccgc gagctgtacg
    18841 cgatcgaatg cgtccaacag ctcggcccgc tctggaccca gcaccgttga caccagatcg
    18901 gcagcggagg ctgccccctg aggaagctcc gcttcggaca cggatcgcca agcttcgcgg
    18961 agccgcccga tctcttcatt cacgtccgtc tgcttgatgc gaccgccctc gcagagagtc
    19021 gccatgcgcc gaaccgccgc gttgaagtct cggaagttcc ccgaccactt ggcttcttcg
    19081 gagaccgcaa acgcaagaaa acgctccttc gcttcgcggc tgaacctcac ccgcgacccc
    19141 aggacgcgcg cgcactgttc gagctcgtag tccagattgg gagcaaaatc ctcgggtcgc
    19201 tctcgtaacg acggcaagcg gaacgtccac aggttcacac gggccaagag gtcctcccga
    19261 aagcgtccct gagcgaccgc taacgccaaa tcacgattgg tccccgccac cagctgaaac
    19321 tcactgcgca cctcgcgatc ggaccccatc ggataatacg tcttctcctc gatagcgcgc
    19381 agcaacatcg cttgttcgtc cagcccgagc tctccgactt cgtcgaggaa caacagcccg
    19441 ccatgcgcct tcttgagcat gccggagcgt tcgttcgctg cgcccgtgaa cgctcctcgc
    19501 aaatgaccga acagcgtcga catcgcggcg tcaccccgga tcgtcgcgca gttcacctcc
    19561 acgaactccc cctcgatctg gcgccgggcc cgcttcaact cgtaaatgcg cttggcgagt
    19621 tgcgacttgc cggcgccggt agggccgagc aaaagcacgg ggtctttgga tcgcgtcgcg
    19681 acgcgttcga tccgttcgac gagctggttg aaggctttgt tctgggtccg aatgcccgac
    19741 ttcagagagg caagcgcgtc gtggtgctcc tttcggaagc gggacgcgat tcgatcgtac
    19801 ttggacaaat ccaagtcgat gatgacgcac ccgcccgcag ggctcggaaa cgcgcgcgtg
    19861 ggcggcgagg tctgcaccag gcgggccgga atgtgacgcg actcggtcag caagaacagg
    19921 caaatctgcg cgatatgagt gcccgtggtg atgtgaacga ggtagtcctc ggcttccgta
    19981 tcgaacggat agtgagccgc aaagtcgtac aagcgctcgt acacctcttc gagcgcccac
    20041 ggatcttcca agtcgaaggt gtgcgcgctc acgagggtct cgggagagac gttggcgata
    20101 tccgagctga cgatttgagc caaagcctca cagcgctggg gcaccaaaag ctctaagcga
    20161 tccaccaaca gatcttcgtg ttggcagagc gccaccgtcg gcctccagcg ctgccaacga
    20221 ttgggaccct taccttggtc caaggtcgtt ccgagcaagc cgatcactac gagctttttg
    20281 ggggggttgg ggcgcatggg ccaggctatc ctatagtcta ttttccatat cttacgggat
    20341 agaacctatc cgcgccgggg tgcccctgag cccgctttag gtcgtttgag cccgatttcc
    20401 ctcgtagctc ccttcggtcg ctcgcgctgg cacgacgggt gcacagacgg gtgctcggaa
    20461 tcgacatgga ttcatgcaat caatcccgtt cttacgatgt cttctcgagc acccatggct
    20521 cgcccgtgaa ggcctggacc cgcggtgttc ccttcgaaga aagcgcgaaa gcacagctcc
    20581 agcgcatcgc cgagctgccg ttcatccata agtggatcgc ggtcatgccg gacgtccacc
    20641 aaggcaaggg tgcgaccgtc ggcagcgtga tcgcagcgcg cggcgcggtc atcccggccg
    20701 cggtgggagt ggatatcggc tgcggcatga tcgcggtccg aacgagcctc tcggcggacg
    20761 atctcccgga cgacctgcgt gcgttgcgct ctgcaatcga agccgcagtt ccgcatggtc
    20821 gcaccgacaa tggcggctcg aacgaccgcg gagcctggca cgacgtgccc gagtcttgcg
    20881 cccacgcgtg gtccacgctg cgcgcgagtt acgagcagat cgtgagcaag catccgagaa
    20941 tcgggcgcgg cccgagcttt actcatttgg gaacgctcgg aaccggcaat cacttcatcg
    21001 aaatgtgctt ggacgacagc tctccctccg cccgagtctg gttcatgctg catagcgggt
    21061 cgcgcggact gggtaaccgt atcggcagct acttcatcga gctcgcgaag caagaaatgc
    21121 gtcgttggtt catcaatttg cccgacgccg acttggccta tttgcccgaa ggaacacgct
    21181 attttcaaga ctacatcgag agcgtcgcgt tcgcgcaaga gtatgcgcgt tggaaccgtg
    21241 agctcatgat gcaagccgtc gtgaatgcag tgcgacgcac ggggttgctt ccgccgttcg
    21301 aagcctcgct cgaagccatc aattgccacc acaactatgt ggcagtcgaa caccactacg
    21361 gagagaacgt gtttttgacg cgcaagggtg ccgtgcgagc ccgccacggc gatctcggca
    21421 tcatccccgg cagcatggga gctcgctcct atatcgtgcg cgggctcggt aaccccgaga
    21481 gctttcatag ctgcagccat ggcgcgggcc gcgtcatgtc gcgaacggag gccaagcgcc
    21541 gcttcaccat cgaacagcac gcggaggcca cggctggcat cgagtgccgt aaggacgctg
    21601 acgtgatcga tgagaccccc gctgcgtaca agtcgatcga ccaagtcatg caggctcaac
    21661 gtgacctggt cgaaatcgtg tatacgctca gacaagtact gtgtgtgaaa ggctgagtta
    21721 atcacggctc acccgcctga agaacgagtg acaggccacg actagacctt gcaaaaagct
    21781 gattgcctcg gaccatagag ataagtcatg ttgattctgg acggctcccg cggtgaaggg
    21841 ggtgggcaaa ttctgcggac ggcgctggcg ttgtcgctcg tcacgcaaca gccctttcgc
    21901 attacgaaca ttcgcgccgg gcgcagcaag ccggggctct tgcgtcagca tctcacggcc
    21961 gtgcaagcgg cggcggccgt ctcgggcggg acggccgagg gggctgcttt ggggtcacgc
    22021 gagctgacat ttcacccggg ctcggtgcac gcgggggact accgcttcgc ggtgggtacg
    22081 gcgggcagcg ccactctggt gtttcagacc atcgcccccg ctctgcttcg ggccagtcag
    22141 gcgagcacgc tgctgatcga aggcgggact cacaacccgc aggcgcctcc gttcgagtct
    22201 ttgaacgagg cgttcctacc ttgcctgcgg acgatgggct tcgaggtctc ggccgagctg
    22261 gagcgacctg gcttctaccc cgcaggcggg ggtcgattcc gagtgaagat tgctcccgct
    22321 tccgctctgc gtcctctccg gctgctcgag aggggcgcgc tgaagtcacg cctcgcaagc
    22381 gcgctggtga ccgggattgg gagccagatc gcgcaccgcg agctgcgtgt aattgggcag
    22441 cgattgggtt ggtcgcgcag cgagctgcgc tgtgaaccac tcgacgagtc gtacgggccg
    22501 ggcaatgcgg tgctgatccg catccagagc gaaaacatca ccgaggtgtt cgccggcttc
    22561 ggacagcgcc gtctacgcgc ggagcaggtc ggcgaacagg tggcgtgcga ggcgctcgag
    22621 tacctcgaga gcggcgcggc ggttgggccg cacctggcgg atcagctgct cctgccgatg
    22681 gcgctcgcgg gtgggggtac tttttcgtct acctccccct cttcccacac gctcactcag
    22741 gccgagacct tgcgattgtt cttgggcatc gagacgcgca tcgaggcgga gacggaccgc
    22801 cggtggcgtt tggaggttgg ggcttaactt cggggtcgcg tgtgcgttat tgggcgccgt
    22861 ttgcgcggcg gcggtgggtg actcggtgag gtgcggctgc cgcgcgacga cgatcgacgc
    22921 gcggcgcgcc cgtcatttcg agcggacaaa cagctcggtt tgggtcgcat acgggcgagc
    22981 ccagagccat gctgtgtcgg gtgacaaaac tcgtttcgat cgggcgccgc acggaggtga
    23041 ccacgccacc agacttggta gcgttgctgc tcgagtgcca tgagcgaatc cgaactttcg
    23101 cagggatcgc cgtggacgtc gcgcggcgtt cggatctttc ctcctccgac gtggcggagg
    23161 cctgccggcg ttgtattcgg tacttcgagc aggcgctgcc gctgcatgtt ctggacgaag
    23221 agcggagctt gctaccgcgc ctgtcgcaaa cgaacgagga ggttcgagcg gcgctcaccg
    23281 ccatgcacaa tcaacatcac gctcacgaac ctcagctggt cgagtttttg gccgggctcc
    23341 gcgacctcga gcaagacccg ctgagcggag acgcgagggc tcgcttggaa cccgtcgccg
    23401 tcgagctcgc cgcgcagttc gaggatcact tggtgctcga ggaacgcgtg atcttcccgg
    23461 cgatacggga gcacctgagc gtcgccgacc aaaccgaagt cttggccgag cttcgcgccc
    23521 gtcgtcagta agcccggccc tgacggcgcg agcctagagt tcgagaccct acccggcgat
    23581 tcgtccgcca ggcgaagtcg attttcaaat ggcgcgcgcg gggcgggagc tctaaccctg
    23641 catgcggaac atttcgggct cggtgccgag ctggattggt gcgtcgtggt ttttactgat
    23701 gacactcgtc ggctgctcgt cacgcgccga tgaacccggg agcttgccga tcgcggggca
    23761 aggcggattc acgaacaaac cgggcacagg tggctcgact gaatccaacg cgggcggtgc
    23821 aggatcaggc gggatcgctt ctggcggcac gacgacgatc ggcggcgcca gcatgggcgg
    23881 cgttcctacc ggcggtgtgg cctccgcggg cagcaccgcg ggaggtaaag ctcagggagg
    23941 ctctagtcag tcgggtggcg ccgcaggcag cgccggttcg ggcggcaaga gcgccggatt
    24001 cggcggtgtg ggaggcgtct cgaacagcgg ggggaatgcc gggtccggtg gtcgcgccgg
    24061 atcgggcggt aacgcaggtt cggcaaccgg aggtattggc gccggaggta ttggcgccgg
    24121 tggtattggc gccggtggcg cagcgggctc cggtgggacg gtcgccaaca ccggaggttc
    24181 gacgagcagt gctggctcga ccggtcagac acgggacatt ctgatccccc cgagcggtgc
    24241 ttggctcggc atgtactacg gcgacgctac gctcgccgcc acccagcaga aactcggacg
    24301 ttcggtaccg ctgcacctgg tctactacgc gtggagcgac gacttcaccc gcgatgcgac
    24361 cgcggaagat cttcgtgctg gccgtgtccc cctcatcaat tgggagctat tcgacatcac
    24421 gctggacgag attttgagtg gaagtcagga cacgctgatt cagaagcgcg cgcaggcttt
    24481 gaagcagctc gccaagccgc tattcttgga cttcggggcc gagatgaacg gcgattggag
    24541 cccgtggggc ggggctcaga acgggaagtc cgcggacaag tacattcagg tgtatcgaca
    24601 cgttcacgat attttcgagc gtgtcggcgc gagcaacgtg atctgggcct ggtgccccaa
    24661 cgtgaccgat gtgccgcgcg aaccctggaa cgaggcactc gattattacc ccggcgacaa
    24721 gtacgtggat tgggtctgcg tcgatggcta caactggggc gataccaacg aaggcgtgtg
    24781 ggagagcttc gcgacggtgt tcaaggacat ctatcccaaa ctcgcgagca agaacaaacc
    24841 gatcttgatc ggagaaatgg cctccgccga gatcggcggt aacaaagccg cgtggatcga
    24901 cgaaatgatt ccgactctga aggatcgttt tcccgccatc cgcgccttgg tttggttcga
    24961 catcaagaaa gagacggatt ggcgaatcgg ttcttcgacg gcctctctcg cggctttcac
    25021 ccgtctggcc aaagatcctt acttcaaccc gtgagttctc cggggtatgg ccgcgcgagt
    25081 ttgcacggcc gcgccggttc gaacgacgcc gactactccg cccaataacg gaaccagtcg
    25141 atttgcatcg tcagatcccg ctggggaaag tcggcgtcgc cgtcacgggg aaaccagagc
    25201 gagtccgggt gccagaggtt gtaaatgact tgcacgggtg gctcgggcac atggctcgaa
    25261 tcggcgagag tccataacgt tcgctcggtt ggatcggagt ccaaaaccac gaagaagcga
    25321 aggatgccag catgccactc gaagcccaac tcgtaaaact gattgggggc aaaccaaccg
    25381 tcgagacgca gggctgggtc cgtacccgca aggacgaagt catcccgatc gtccgccgga
    25441 gtctcgtaat agcggccggt tgccaagtct acgacgcgcg ccagcttgcg gaatagttca
    25501 tccccttcag ggctaacttc gtaatcggta aacacggtga gataggccaa agacgggtcg
    25561 ccgcacagca cctgaaaatc aatctctacg tcatcgcgca ggccgtttgc gttgcggtct
    25621 gccccatcgt tgtagaaacc gagcatggcg tgaacgacct gctccgaagg ctcgcagcgg
    25681 ccgaaggcca gtcgcgaccg ataggttccg tagccaagat tgcggacaat cgtgatctcg
    25741 gtgcgcgagt cgacgccgga gtaatcagcc ggaccaaact cggggtgccc cggccaacga
    25801 agttgcgcag ccaagccatc actcgcggcg gcggcttccc cgctcagttg tacgacagag
    25861 ccaggcacgc tcgcgcgcga cgagagcggt gaagggtcgt cgttcgcgaa ctggacctcg
    25921 aacgtgggcg gccgtgccgg aagcgcagcg actccgacgc tcccctcccc gggcccacag
    25981 cccgaagcgc cactcatgca aacgagcgag agtagggcca ctctcgcgat ttgcagcgaa
    26041 ggtttgatcg aatgcagccg cgagctcgcc tccctgtaag ggagctcagg gcgagaggta
    26101 gcgacgcaca tcggcggcat gggggctctt gggatagttg cgcaggaaga gctcggcgga
    26161 ttgcgccgcc gcttgctttt ggccactccg ggcgagtgcc tccacataga gagcctcacg
    26221 ctcctgcatc aaagggccgc gcggaaaacg cgctcgcccg cgacccaaga ttgccaacgc
    26281 ctgactggca ttgccagtgc ggagcgacga tcgagcctcg agcagaagtt ggctctcttc
    26341 gcgcagcgag ctcgcctcgg ttgcggcggc ttttggttct tcgacggaga ccgccgggcg
    26401 ggcggacttg ggagacgccg gcatttcccc agcgcgcgag gagcgcgccg atgtcggcac
    26461 ggagttcacc gagtcggcct gaagttccgc gacacccgtg cccttcttcg cgtgaaccaa
    26521 ctgctctctg cccgcgctcg cggagggcac gggatgatcg agagcagact cgttctccgc
    26581 agcgggtgca tgtccgcgag catcctgctg cgctgccgtt ccgttcgtgg agacgttcga
    26641 gcccgccccg ttcgaagcag agctcgccgc gcccgagtct cccctgttca cgctcggcgc
    26701 ggtgatcgcg accggagaag agacgatgac gcgcggcgcg gaccgcaccg tggcatatcc
    26761 cgcgatcccg agcgcggtgg cgccgacgat ccaagtgagc cccttaccaa tcgatagccc
    26821 cgccccggca ggcagattgg acgccggggg gctcgggagc tcgggccctg gctcgggccg
    26881 agcggagggc tccgtgtgcg gcgatgctcg ccccccgggc ggggccggag gtcccggtaa
    26941 attggctacg atgttgctcc agagctcttg tcgttccgtc gccgagagct gctggtgccg
    27001 cgccgcttcg atgagctccg ccgtcagaga ctctggctcc agttcgacga ggcgcttctg
    27061 ggtctcagtc atgacgcctc cccggtgcgg cactccaaga agtgctcctg agcctgcatg
    27121 cggctcaccg ccgcacggaa caacttccgc gcgctgtgca accgcgaata gatggtgcca
    27181 atcggggctc cgagcgcctc actgatttct tcacccgaga agtgctcgac ttcgaacagc
    27241 acgaagacgg ttcgctgatc ggcagacata ccgtcgagaa tcctttccaa aatgagcaag
    27301 cgctcgtggt gctcagcgac ccggccaacg tccgcgcgca agtcgacggt ctcctcgagc
    27361 tcgccatctg ccgcgagccc aggcgccaaa cgggcgcgac ggcgccggtc ccgagccacg
    27421 ctgcgagcaa tcgcaaagag ccaggtcttg atcgagctcc ggccctcgaa ttcgcccaag
    27481 tgcgcatgca cgcgcaaaaa tacgtcctgc accgcatccc gagcttctgc ttctgcaacc
    27541 ccgagccgca acaccgtgcg ccagacgaag tcggcgtgct gttcgtagat ttgctgcagg
    27601 ttcatggttt gctgcggctg aatcgcttac ccattagagc gccgaggcaa acggattcat
    27661 ttgggaaagt tctacagtgc ccaatctacc ccgaactcca agccgaggtc ccccgcgatt
    27721 cctggagttt gcagcgggcg gcccccgttt tcgaccgtga ggctgagccg acgcaggggg
    27781 atccaggctt ctgctcgaac ctggacgaag atcgctcgcg tcaaagagag cctcccgagt
    27841 aagccgggcc ccaatgcgct ccaaaacagg ctcgtcgaac ggttctccga gtaatcgtgg
    27901 cccgctccgc gcaatgctcc aaaaccatac tgcacgcagg gagcccaacg caccgtactc
    27961 ccaacgaacc ggcaaatctg cactctcccg aaggtcaggc tggtctccac gcgcccctgc
    28021 gcgagtgaga gttcgggcgc aaacatgcgt tggatgctca agcccagctc taggcccagc
    28081 gggccgcgcg cttggagtcc tcccccaatc atccaaccca attcgggcac gaacccgact
    28141 cccacgcccg cccccgccgc caacgcgagc ccccatggaa cgcgcggctg ctctcctgcg
    28201 gtgttgtcgg acttcacggc tttgcgcccc gcattaggct gattcgacac cgaactcggc
    28261 tcgagtgggg gttctcgctc gccccgctca atcacgcgat cgaggaccaa agccaacgtc
    28321 gcggtcaccg cgtccgccaa tgcggcgcag ctcgcgctag gatctcgcag gtttcgttca
    28381 ccgcgtttgg agccgtcgaa tcgcagcact gcgaagaaag ctcctcgccg tcgatcgaaa
    28441 tctacggata cgcggagggt ctcggccgcg tcacgtgttt ccgcgagtgg tcgtcccagc
    28501 acctgactga tgcgccgctc caagtccaca ctatccggac aatccgcggc ctgtagacta
    28561 cgggcaacct ccagccgcaa cggctgcact gcgcgtgccg gggacagcac gaggatcgag
    28621 ccgagcccgg ccaccgccgt gcaaccacca attttcgtcc aggcacgtcc ttgcaacgcg
    28681 ctgcggaagc tacgtggaag ccgcgttcag cgcaagcgcg tgttcagccc gcggccgaac
    28741 cttcacccag gtaacgtacg acgagcaaag tcacatcgtc atccaggctc gcgctccagc
    28801 tcgagaccgc ctcttgcact cggtctcgga ttacagctgc aggctggcgc gccagttcct
    28861 cgatgtaacg caccagtctt tcgagcgaga actgttcgtg acgggtgttg cgcgcttcgg
    28921 tgattccatc cgtgtgtagg atcagcagat ctccgggcga gagcacgagc tcggagtcct
    28981 gagtcatgtg gcgcacgctc gggagagcgc cgacccaaaa ccccggcgta ggcaccaact
    29041 cacaacgacc cgttcggctg cgccaaacaa tcagatcttc gtgcgcaccg gcgaaggtga
    29101 accgcccgct gccttgataa tggagcaccg tgagcgtggc atggtcgtct cgcttcaaac
    29161 gcaggcgcac gctgtcccag agcgcgtcgc cgacggtgga gaccacctgg ctgggcgacg
    29221 gcgcgggctg catgcgcgtg agccccgcga tcatgctttg gatcatcagc atgattagac
    29281 ccgctcccaa gccgtgcccc gtcacgtcgc caattccaat ccacgcgcca cgcgcggtgg
    29341 cgatcacgtc gtagtaatcc ccgcccgcct cggccgccgg caccatcaag gccgcaactt
    29401 cgagacccgg gatctcggtg aacactgggt tgatgctggt ctgaatgacg gcggcgatcc
    29461 gcgcctcctt tgcgactcgg tccgcttcga gctgagcacg cgaggccacg tgcgcgacca
    29521 tttcgcggtg caacatggcg cctttgatgg cggagccgat ttgctgacgc agcggctcgt
    29581 acacactgga ctgagcatcc cccgcaaaca cggcgagtcc gagcatctcg gtctcgaacg
    29641 tgatcggcaa cgcgacgaag tgctggcagc tcgccgtatc aaacagggcg cgcggcgcga
    29701 gctccgtctc cgggaacgcg ccgtcctcga taccaggctc gccgtcgtcc gttttgatca
    29761 ccagcgtctt cagctgaccc gagagttcgt ggctgtgcag cgataccgct ccgcgactga
    29821 tctgcagctg agccaggccg tcgaagatct ccttcttcaa aagcggcaag ctcagcgtgg
    29881 tggccagccg ttcaccgacc caacccaggc gagaattggc gatctgttga tcgatcttct
    29941 ggcgaccgag caagcgaatc gaagccgcgc ccaccaacac gcgcgcgtcg tgccaaatgc
    30001 gctccaaccg cgagctgtct tcctcactcc gcgaggtgcg ttggaactcg gctctcagca
    30061 ccgagacgac ccgctgaaac tcgtctaggc tcgccccttc ggattgagcc tcttctagca
    30121 gctcctcgaa caggccgttg aagcgccctt ccttgccgtc gagctcctcg tcgagcgcgt
    30181 tcaggagcag atcgggccag ttgcctaggg cgtcggtggg aa
//
LOCUS       DASTKU010000120         5549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_23244,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000120 DASTKU010000000
VERSION     DASTKU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23244"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            421..1479
                     /locus_tag="VFQ61_04275"
     CDS             421..1479
                     /locus_tag="VFQ61_04275"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET9953691.1"
                     /translation="MFPTVDDVRIESLRPLLPPAILMEELPLGESAAERVAAARAAVS
                     EIVSGKDDRLLVVVGPCSIHDPDAALEYAAKLEPLARAHCKELCVVMRTYFEKPRTTV
                     GWKGLINDPDLNGRFSINRGLRIARKFLIDVVEVGLPTATEFLDPITPQFMADVVSWG
                     AIGARTTESQVHRELASGLSMPVGFKNGTSGAVQTAVDAVRSAASAHHFLSVTKQGLA
                     AIVATRGNPHCHIILRGGENGPNYAEEHVQQATRALKKAELRPSLMIDTSHANSGKDP
                     SRQPLVAAEIARQIELGSHAIFGVMLESFLLEGRQDVKPGKSLLRGQSITDGCLSFDA
                     TQPLLERFAKAVITRRRG"
     gene            complement(2172..3608)
                     /locus_tag="VFQ61_04280"
     CDS             complement(2172..3608)
                     /locus_tag="VFQ61_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953692.1"
                     /translation="MAKTGMRAVRSVAIWFTSLGLFTWACGDSAKDVKGGLGGLCAKD
                     GDCQASLACAFGTCHKTCKDASVCGVGLLCVNGPTSGVCQLPSEAVCSQTQACPIGQK
                     CSADGQCRDSCDSGNSCSNGLICSAFKLCERPDDVGTGGTPSTQPGEGGESGRADGGS
                     AGSVPVGSGGASGAGGGEPTGGVTAEGGAPVGSGGDTSATGGDAGAGGVGPLVSGESC
                     DSDDRGNDADSAHVALPGVRVQSCLQVKSDVDFFEFTAPAAPQQGGYFVLSATDVAAS
                     AALRMTLESAAGGSALGTWYGEPGESVFAWVAAAPGASFRVAVSGESWAGAANFVFRA
                     RYAGVRDDFAPNGTVEEAKPIDVDNSIQGYAFGGYNGPTAPEATDFDDFFAVHLNAGK
                     LKARLSPPADGRAEIRLLDKNGEEVAGGRVFHGLEGQPIELIMSKIVEGDYILRVMLF
                     GDMPESVGGGARPAPHVTDPYTFTVMQP"
     gene            complement(4740..4811)
                     /locus_tag="VFQ61_04285"
     tRNA            complement(4740..4811)
                     /locus_tag="VFQ61_04285"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4777..4779),aa:Val,seq:tac)
     gene            5095..5168
                     /locus_tag="VFQ61_04290"
     tRNA            5095..5168
                     /locus_tag="VFQ61_04290"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5129..5131,aa:Asp,seq:gtc)
     gene            5469..5545
                     /locus_tag="VFQ61_04295"
     tRNA            5469..5545
                     /locus_tag="VFQ61_04295"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5503..5505,aa:Asp,seq:gtc)
BASE COUNT          991 a         1802 c         1643 g         1113 t
ORIGIN      
        1 cggttcatgc tcgtgcccgg cggaacctgc cggccgctcg aggttccgcc tgttcccgga
       61 cctcacccct cagcctccag tgggtccgct tctcgtgatc tcctctccta agttctcccc
      121 cagagatcct caactcggcg cctttaggca gcgcgcctct cgatctcggc ttttcgaggg
      181 gaaccgcgcg ctttcgtggg gcgtgcggtg ggacctttcc gtgtgttttc agggtcgagg
      241 cgagcctgcg gtgcgcgacc gctgccccgc tcacgacgcg gtgggccttt ttgcgaatcg
      301 gccgcggttg ctggctcttc gttttgcgtc gagcgttgga gcgacacgtc cttacccctc
      361 ggggcgactg cgcgctgcgg cgagatcgcg gaacgtcaga gctcgagtat acattgcggc
      421 atgttcccta ccgtcgacga cgtccgtatc gaaagccttc gcccgctact cccgccggcg
      481 attctgatgg aggagttgcc tctgggtgaa agcgccgccg agcgcgttgc ggcggctcgg
      541 gctgcggtct ccgagatcgt ctctggtaag gatgatcggt tgttggtcgt ggttggccct
      601 tgctcgatcc acgatccaga cgccgcactc gagtatgccg ccaagctcga gccgctcgca
      661 cgagcccatt gcaaggagct ctgcgtcgtc atgcgaacct atttcgagaa gccgcgcact
      721 acggtggggt ggaaggggtt gatcaatgat cccgacttga acggaaggtt ttccatcaac
      781 cgagggctgc ggattgcccg aaaatttctg atcgacgtcg tggaagtagg gctgcccacc
      841 gccaccgaat tcctggatcc aatcactccg cagttcatgg cggatgtagt gagctggggc
      901 gccatcggcg cacgcaccac ggagagccaa gttcatcgtg aactggcgag cggtttgtcc
      961 atgcccgtcg gcttcaagaa cggaacgagc ggcgccgttc aaaccgcagt cgacgcggtc
     1021 cgatccgccg cttccgcaca tcatttcttg tccgtgacca agcaaggcct ggcggcgatc
     1081 gtggccacgc gcggcaaccc gcattgtcac atcatcctgc gagggggcga gaacggcccg
     1141 aactacgccg aggaacatgt gcagcaggcc actcgagcgc tcaagaaagc ggagctccgt
     1201 ccgagcctga tgattgacac cagtcacgcg aatagcggca aagacccgtc gcggcaaccg
     1261 ttagtcgctg ccgagatagc gcgccaaatc gagcttggca gtcacgcgat tttcggcgtc
     1321 atgttggaga gtttcttgtt agagggacgt caagacgtca aaccaggcaa atccctgctc
     1381 cgcgggcaga gcatcaccga cggttgcctg agctttgatg ccacacagcc gctgctcgag
     1441 cggtttgcga aagccgtcat cacgcgcaga cgcgggtgat ggcagatata tttacctctc
     1501 gataacgcgg gtgacacatc ggacggatag cgtccgatgt catggacctt cgtaaaagca
     1561 ctcctcgcaa tgctcgcgtt gcagctggtt cgggaagtga atcaatggca atgatggttt
     1621 gctcccagcg cgagggagca agcaccgtgc ggagagatcc ggggcggtac ttcagcgtcc
     1681 cggatcctgt gattcggctt ccctcgccat ccaacgtgcg caacatggcg cgagcgcatg
     1741 ctcgcagctg cacgttctcg ccggtggttc cgctaccgcg ccgctgggct cgcgccaaga
     1801 attgggcaca gcactcgcgc gcagggcgct tggacgcccc cgcactttct gtcatgcgcg
     1861 agctcgagtg ggcgaatcgc ggctgactcg gcggctgtcc gttatgtctc tgcagtttcg
     1921 gcagggcggc gctgccttat tgggtcacgc gcggctgcct tgagccttac ggcggagagc
     1981 acgctcgttc cgcggcgtgc cacgctcaag gctctccagt ttcgaggggt ccttttggcg
     2041 caatcgcgcg cccaatcagc cccgaggagg cagcgagtga tgccgttcgg agcgcgcggc
     2101 agcgcacgct ggctcgcgcc acgctgagcg tgcaagggaa cgcgcggtcg cgccccttca
     2161 cgggctcgtc ttcacggctg catcacggtg aatgtgtacg ggtcagtcac atgcggcgcg
     2221 ggccgggccc cgcccccgac cgactcgggc atgtcgccga agagcatcac acgcaggatg
     2281 taatcgccct cgacaatctt ggacatgatg agctcgatcg gctggccctc caggccgtgg
     2341 aatactcgtc caccggcaac ttcctcgccg ttcttatcca gcagccggat ctcggcgcgg
     2401 ccgtctgccg gaggactcaa acgtgctttc agtttgccgg cgttgaggtg gaccgcgaag
     2461 aagtcgtcga agtccgtagc ttcgggcgcg gtcgggccgt tgtagccacc gaacgcgtaa
     2521 ccctggatcg agttgtctac atcgatgggc tttgcctctt cgaccgtccc gttcggagcg
     2581 aagtcgtcgc gaacacctgc gtagcgcgct cggaatacga aattcgccgc tcccgcccag
     2641 ctttcgcccg aaaccgcaac tcggaacgac gcgcctggcg cggcagctac ccaggcaaac
     2701 acgctctcgc ccggctcacc gtaccaggtg cccagagcgc tcccgcccgc ggcgctctcc
     2761 aatgtcatgc gcaacgcagc gctagccgcg acgtccgtcg cgctcagcac gaagtacccg
     2821 ccttgttgcg gtgcggccgg cgcagtgaac tcgaagaaat cgacgtccga cttgacctgc
     2881 aggcaggact gcacacgcac gccaggtaag gccacatgcg cgctgtctgc gtcattgccg
     2941 cggtcatccg aatcgcagga ctcgcccgac actagcggac ctactccgcc cgccccggca
     3001 tctccaccgg tagcgctggt gtcgccaccc gatccgactg gcgcaccgcc ttctgcggtg
     3061 acgccgcctg tcggctctcc gccacccgcg cccgacgcac cgccgcttcc gactggaacc
     3121 gatcccgcac tcccgccgtc ggcccgccca ctctcgccac cttctccggg ttgagtgcta
     3181 ggggtgccgc ccgttcccac gtcatcgggg cgttcgcaca gcttgaacgc gctgcaaatc
     3241 agcccgttgc tgcacgaatt tcccgaatcg cagctgtctc ggcactgacc atctgccgag
     3301 cacttttggc ctatcgggca ggcttgtgtt tgcgaacaga cggcctcgct cgggagttgg
     3361 cagactccgg aagtcggccc gttgacgcac aacaacccta ctccacaaac cgacgcgtcc
     3421 ttgcacgtct tgtggcaggt cccgaaggcg caagcgagcg aagcctggca atcgccatct
     3481 ttcgcgcaca aaccgccgag tcccccctta acgtccttcg ccgaatcacc gcaagcccag
     3541 gtgaatagac ccagactcgt gaaccaaatc gcgacgctcc gtaccgcgcg catgccggtc
     3601 ttcgccatgc attccctctg gcagaaaccg tactcgaggt gcgcgacacg cgcaacactt
     3661 cgcactgctc tttcaacgaa cggacgtcac tacactcgac cgtactcaac accaaaaggt
     3721 gtgcttcgcc cccaactcac atcaattcat gtgggtgtca gtaagccgag aagcgcagtg
     3781 aagcgactcc ttcacacacc aaatgggtcc acattacttg tacgttctcg cggcttcgtg
     3841 ctgtcccgtt cggacaggtg atagtcccaa attccgcgca ttgtttgcgt gtttttgaat
     3901 ctcgagtggc cgccaactcg ggtgccgatc ggctacagcc tgtggcggtg tctggggctc
     3961 tgaagtctga tcgtgatcaa cgcctcagtt caggtccagt ctccctcgta ctcggtctca
     4021 cggtcatcag gcgttgatcg tgtttctggc gtgcacgcct ccatccggcg tttggaagtt
     4081 cacggtatgc ggtgctcgcg ccgtcgctcc agcggaggcg ccatccgata actcttaggg
     4141 gttattatcg gaagtgttcc tgcttcttcg aattgcgttg cccatcgtta ctggtgctcg
     4201 gccgagtgac gagaagccac gtcaagagcc agcaaaccag ctcagtcatt gggctcgtat
     4261 gcttcgagca ccgctgggca cgattgcgct gaggaggcgg gatgagtcgg atgagtcgga
     4321 tgcccgatcc cgtgtcagtt cgaccccagc agccgaatat aggctgctcc ctgagaagcg
     4381 aattgcgagg ctcgccgagc tgcgaattct cgccagcagt ttcggcgtcc gcaacagcgc
     4441 gatgcgcggc ctctgtgtcg tgtgacgctc agagtcggag aggtacgccg ttttcatggc
     4501 gtccggctcc tcgcacttca gcgaagatca cgcagctagc cctccccaac ctgtgcggct
     4561 ttgctcgagc cttgcggctt gccttcacag agagcagctc gaaatcaatc ggtgggagct
     4621 cctctacgga gcttggcggg accgcaaatg cctttggagc tttcgccttc taccttgggc
     4681 tcgcattcgc taagtcttcc ggctcagcag ctcggaacac acgaaggcgg atatgattgt
     4741 gggcgggaca gggcttgaac ctgcgacatc cggcttgtaa ggccagcgct ctaccgctga
     4801 gctacccgcc ccttccaggg gccactcttt ccagcccgag ccacctcgtt tccacgaagc
     4861 cgtttccacg aagctcgccg tgccttccag agcccgcgcg cgacgacccg cgagcggggc
     4921 cgtcttatta cacaagccgt gcggcttgag aacgatccct ttctgttttt ggtcgaagga
     4981 cgctgagcca ataaccagct tgaaccttgc ggccagtcag aaagaaaaag ttcgccgaat
     5041 gttttctgtt gtggatctca ggaggagctg tggtattaac cgccccgccg cttcggggcg
     5101 gtagttaagt tggttataac gccggcctgt cacgccggag gccgcgggtt cgagtcccgt
     5161 ccgccccgct agttcgatcc ctgtgtgttg ctcgttgcct cggctcaacg ccaaggccgg
     5221 tgcaataaat tctgtaacgg cagtatttcc gtaacggaag ttcagggtac gaacgaccga
     5281 gtgctgcagt tcggacacgg gtagttgttc atcgtaacgc cggaacctgg tttcggcgct
     5341 gagcctctcc cattcgaatc cacagcctca tctagccaga aaatagaagc tacgagtgag
     5401 ctatccacag gatctgcaca gcagatccgc ccacaattag gatctgcaca gcagatccgc
     5461 ccacaaacgg ggcggtagtt aagttggtta taacgccggc ctgtcacgcc ggaggccgcg
     5521 ggttcgagtc ccgtccgccc cgccagttc
//
LOCUS       DASTKU010000121        18529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1137465, whole genome shotgun sequence.
ACCESSION   DASTKU010000121 DASTKU010000000
VERSION     DASTKU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 18529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18529
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137465"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /locus_tag="VFQ61_04300"
     CDS             complement(<1..1900)
                     /locus_tag="VFQ61_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9953693.1"
                     /translation="MDRRAFIRSSVLASATAAATRMAQGDIRAKELGSKDNRPALDDS
                     VRERNGDGVQWDKAPCRFCGTGCHVQVGVKGGKVVAIAGDARAEVNRGLLCMKGYHVG
                     LALYGKDRLTQPLLRKNGKLSPISWDQALDIIASRVLANPKGFALYGSGQWTIPEGYA
                     GNKLMKAGLSNNHIDPNARLCMASAVTGFLATYGVDEPAGCYDDLDRCDVLILWGNNP
                     AEMHPVLWSRVIDRRARGEKVVIIDLATRKTRSTAQADRHINFIPQGDLAVANGIMNL
                     LLTSDRYDKEFVAKHVAFRADSDPPTLAGKPIDFQEFKRGLAEYTPKRVEELAGVSEA
                     DLRYLADLFARRDLRITSTWCMGMNQHTRGTAINCLVHAVHLLSGHFGRPGDAPMSLT
                     GQPSACGTVREVGTLAHALPGGLVVENAEHRKHAEEYWNVPEGRISGKPGYHAVEMWK
                     RFSTPQDQGGDIDTLWVQVTNPGQTLPNSHALFEASRKIPGKFLIVSDVYPTVTAQAA
                     DLVLPSAMWIEKNGMFGNSERRTQQWFRMVKPPGDARDDCWQLIALARRLYERGHAGM
                     RDKDGKFLFAIEKDGKEVPIWDFAHYYDVNVDEHLFNEYRKFSRHKHKDLAPYSEYVK
                     ARGLRWPVV"
     gene            complement(1904..2206)
                     /locus_tag="VFQ61_04305"
     CDS             complement(1904..2206)
                     /locus_tag="VFQ61_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953694.1"
                     /translation="MPCSGLVLTMRDVSSIGELRRALADDSEITFGEACGVRLPLVVE
                     TPTLDASRVRHEQLEAWPGVLQADLIHLDQSDLDLIRESEAAPTSTEVQARAGRDS"
     gene            complement(2374..2562)
                     /locus_tag="VFQ61_04310"
     CDS             complement(2374..2562)
                     /locus_tag="VFQ61_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953695.1"
                     /translation="MRTVTARRAVSTTYAKADLEPKIDPSRAASRGISRSAERWWTQS
                     PRALTHELIRGTCRQSEV"
     gene            2586..3173
                     /locus_tag="VFQ61_04315"
     CDS             2586..3173
                     /locus_tag="VFQ61_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953696.1"
                     /translation="MLALWGCNRPIKEVPRARAPSAARYPVEIRRPAKLSSIETGRAD
                     ALGREQRVPCVTCHSLGVRRPVPESSAELEEFHRGLSIEHGSLRCASCHSAEGAELLK
                     LADGRSLPITDSIEKCGECHGPQYRDYRHGAHGGMLGYWDLSRGGRLRNTCVDCHDPH
                     IPKPPPVMPVLPPRDRSSTAHSVQPVAEARGAAHD"
     gene            3166..4068
                     /locus_tag="VFQ61_04320"
     CDS             3166..4068
                     /locus_tag="VFQ61_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9953697.1"
                     /translation="MTEPKRRVALKVLGATLGAAAFARALAPLQSFAKALSLDEFLQQ
                     HYRELSSEDLQIIFRRLEAQTARDYGANVHISDPRPIEGVEFAYALNLSVCIGCRKCV
                     EACHIENNHDRASNNSYIRVLEMEQGSFDMEKGNAAYDHPVPAPGKYYMPVQCQQCDN
                     APCVKVCPVQATWKESDGIVVVDYNWCIGCRYCEAACPYHARRFNWTKPEIPKDEINP
                     NQGYLSNRIRPQGVVEKCTFCLHRTRNGRLPACLEACPTGARVFGNLKDPNSEIRWVL
                     NNKRVFVLKEELGTLPRFYYFFDK"
     gene            4102..5403
                     /gene="nrfD"
                     /locus_tag="VFQ61_04325"
     CDS             4102..5403
                     /gene="nrfD"
                     /locus_tag="VFQ61_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET9953698.1"
                     /translation="MAQLTSGTPTPSPAPLQSLPHGEPHLTSYPRFLWHLLQGSFQGS
                     RFFYVWMTALTAVALVGANAWAKQVAMGMGVTHMSDHVSWGLYIANFTFGVGLAAGAV
                     MMVIPAYLYEDHEMHEIVLIGELLAIASIVVCLLFITVDLGRPDRFWHIVPGLGRFNW
                     PISMLTWDVIVLNGYLLLNLHITGYLMYMRFLRREPNPRWYIPFVFLSMAWAISIHSV
                     TAFLYSGLGGRPFWNSALLAPRFIATAFVTGPAFVIVLLQLLQRLGQLKVGAGPIRTL
                     SSVMRITVLVNLFMLSSELFTSLYTGGAHAASVRYLFFGLHGHHALVPWIWSALALNL
                     GSVVLLFVQRVTGRSLFLTLACIAAFSGVWIEKGMGLIVPAFIPSTLHEIVEYTPSLT
                     EWKVTIGIWAAGLLVLTVLLKLALPVLRRSLDSASQAPQKH"
     gene            5611..6231
                     /locus_tag="VFQ61_04330"
     CDS             5611..6231
                     /locus_tag="VFQ61_04330"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET9953699.1"
                     /translation="MQKLVKGIHSFQKGYFATHRQLFEQLAQAGQRPETLFITCSDSR
                     VVPNLIMDAAPGELFIVRNVGNVVPRADLPGGTAAAIQYAVEVLNVENIIVCGHTQCG
                     AMNAVLRPETMESLPFVKRWLAQTSNVKQVIEERYGHLDAEARVTAAAQENVLAQLES
                     LREYPFIRDRLDEGKLLVSGWMFDIGRGKIFDFDPDQSEFVPLLAD"
     gene            6231..6407
                     /locus_tag="VFQ61_04335"
     CDS             6231..6407
                     /locus_tag="VFQ61_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953700.1"
                     /translation="MSRSVDLWNGRPRKNEREARAPARESALVVLIARRNTAQATRKN
                     PDVGITPTSEAPPS"
     gene            complement(6521..8008)
                     /locus_tag="VFQ61_04340"
     CDS             complement(6521..8008)
                     /locus_tag="VFQ61_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET9953701.1"
                     /translation="MGSITPTEADPRRVYALLLELRAEVESLGSELFARWRPRIERLA
                     FQPSALNLAHYLILRRRDLRALQALLPPLGLSSLGRGEARVLANLDSVTATLAATLNV
                     PQGHAPKRPSVARFYLGERLLERNARALFGLGERGRRGRILVTLPSEAAEEPSFIAGL
                     VARGVDCFRINCAHDSPREWGQMIEHVHAARKSSQRSLRVMMDLAGPKLRIARCTPDR
                     VRAHTGDRLWLARGVDCRAPSDPAPRAVLEPSEPAALRDLKVGDSLLIDDGKLHAAVE
                     AVQREGYSLRVIRARYKGQRLAAGKSLNAPDIELALGALTAKDLGDLEFVADHADIIG
                     YSFVQSPSDVEALRETLTSRAKNPRIPLILKIETARAVRNLPEIVVASASQAVTGVMI
                     ARGDLAVEIGYQRLAEIQEEILWICEAAHVPVVWATQVLEHLVKKGLPTRAEMTDAAM
                     AERAECVMLNKGPYVAEAVEVLKDVLTRMSEHQNKKTPTLRRLHF"
     gene            complement(8294..10720)
                     /locus_tag="VFQ61_04345"
     CDS             complement(8294..10720)
                     /locus_tag="VFQ61_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET9953702.1"
                     /translation="MPVSEATQSTLLLGPTNTGKTHRAVERMLSHDSGMIGLPLRLLA
                     REVYDRIAGRLGEDRVALVTGEEKRLPRRPSYWVCTTEAMPLTRDVDFVAVDEVQLAT
                     HDERGHVFSDRLLHARGRKETWFLGSSTMRGVLSQIVPAARLQEHPRLSRLSFAGTTK
                     LSRLKPRTALVGFSMRELYELAGRLCALRGGTALVLGALSPRARNAQVAMFQSGEVDY
                     LVATDAIGMGLNLAVEHVAFASLRKFDGHRPRALSPAELAQIAGRAGRYLQDGTFGTI
                     APLELPLDVARSLEAQSFEPVRSLRYRNHDLDFSSAAELLASLKAPPKLSCFTSAPHA
                     EDLRVLEALVQDPELTPLLAAKGRVELLWHVCQVPDFRNLLFEVHIDLLKGLFVRLCE
                     GPLPRDFMYAQIEELERFDGDPDALMARIARLRTWAFVAHQSEFVRDASMFQAALANL
                     EDRLSDALHAALVDRFVARRGSNKIRRTLKGTPRTGVPGSTLNGNAPPTVDESNGELA
                     FRPFAALSGLRAQLGPVEVEPQLSPTELLSSTLLPLVDAPHEAFRLDERGRIWAGQTE
                     LARLTRGSSLALPDVRLIELPQIGGGLRAQLQRRLIAYVRDVSSRLVGEAHALADHQD
                     RLLRALYYQLHTGLGVVLLKNSGLPIPQLRAGLKTTGLDRHGLRFGQLAVWISGGFTP
                     ARYVERRALLSAQTPEIAAPGSWLPDTLGRPSYVASARHAPQWLALGYVVLGDRALRL
                     DLAEKVAHNLHAHAQNTSDEQTQARSPRTPPQAHAAQQLGSFGVPARERGRALHALRG
                     LVPPAVSLTT"
     gene            10789..11997
                     /gene="galK"
                     /locus_tag="VFQ61_04350"
     CDS             10789..11997
                     /gene="galK"
                     /locus_tag="VFQ61_04350"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET9953703.1"
                     /translation="MNLSELTQAFERRFASSPRLFAAPGRVNLIGEHTDYNDGFVLPM
                     ALELATVVAASPRSDGVLRVYAQNFDETREVSLRVESAAGARSGHWLDYVEGTARVLL
                     RQGVEASGADLWIRSELPLGAGLSSSAALELAVGLALTSLTDQPRDLQSLALAGQRAE
                     HEYVGTQCGIMDQLCVALAQPGYALFIDCRSLAVQPVPLAWRTAEGLREVSVVICDSG
                     VKHSLASSAYNQRRAECESAVRVLRQHAPEIRALRDVSRELLERHATELSPTELARAR
                     HVVTENARTEAAREALLRGELALVGQLMYESHASLRDDYQVSVPELDGLVEIASRVSG
                     VYGSRMTGGGFGGCTVSLCEPSVVSELEAAFTREYSKRFSIAPRTFVSRAGAGVAEVF
                     PPGVPAGGRP"
     gene            12095..13474
                     /locus_tag="VFQ61_04355"
     CDS             12095..13474
                     /locus_tag="VFQ61_04355"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HET9953704.1"
                     /translation="MSKGLFASLVLLACSSSNENAGPGQNPGLGGVAGLGEGTVSGAG
                     GPSTNGGSSGSAGASSPNAGGLGGMGEFPAKGAAPSGGAGSGGTQAQVSSGGRSSGGR
                     SSGGRNSGGGESGAGPVAGASNGGAAGGAPASGGAGSTSAGAGTCVESNCGTHKWACW
                     KMPNPASSPASVPNHQSHTDLGNGAVRDNITCLVWEKANPDKKGNWQSASDRCAALAS
                     SGYAGFDDWRLPTRVEMASISDVTLGAKGYPAPFGVTSGYYVTGSFWYKTILTAGGAK
                     PEDRVWGYGTNGFTSNAIVRSNTDNLARCVRGNGPGEAAGDYAVEPPNHYTLAGSGVD
                     ATVTDNYTGLTWQATYSKARMPWSEASAYCAAQRTAGLSDWRVSTLNELASTVNEALV
                     APAINRTVFPNTSGACDETGWFWAAESSKVGGTAWGLSYCDGYTGWNAGASASAWNYF
                     TDAYVRCVR"
     gene            complement(13501..15642)
                     /locus_tag="VFQ61_04360"
     CDS             complement(13501..15642)
                     /locus_tag="VFQ61_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9953705.1"
                     /translation="MSHPGLLDAPLPRLLLQIGLIVGSSRVLGRLTERLHQPRVLAEI
                     TAGILLGPSLFGWLWPNAYRFVFEPASLPALGAISQLGLILFMFLVGLELDPAMLRGN
                     SGRSIAISHASIVAPFVLGVLLAFGLQAQLRPEGVPFMSFALFMGTAMSITAFPVLAR
                     ILKEQRLLNTRVGNVTITCAAVDDVTAWCILAFVVASSRASGLGSALVTTLLAAGYIA
                     LMFLVVRRTLARLAIRISGREVLTQNVVAATLLVLLVSSFVTESIGIHALFGAFLFGA
                     IVPKQGNYVRALADKLEDLVLVLLLPLFFAFSGLRTSLGLLQSPAAWLMCLAIVAVAC
                     IGKFGASTLAARISGLSWRESSALGALMNTRGLMELIVLNIGLDLGVIGPELFTMMVV
                     MALVTTSLTTPLLHRLYPRSRLQETIAEPESAVPIVNDGFTALICVADSRVGPSLASL
                     ADALCPARDARVYALHLITPSERGSFADEAGPLADATLKPLLERARSSNLPIRSLGFT
                     SIDPAADIANVAEVKEADLVLLGSHKPVLGQSLLGGSVYRVMRLARTDVGVLIDRGLK
                     DVRRVLVPFMGTPHDLTALAIARRILSKPGSEVVILHVIERAPDQAEAAREPLGAREA
                     VDSVFPEGRGSRVRFRVEAAPDPASAILKETRNDYDLVIIGLSRSYGLEERAFGVQRE
                     RLLDQCQTSLLVVRSKSAREPVSTPQAAPLL"
     gene            complement(15847..16413)
                     /locus_tag="VFQ61_04365"
     CDS             complement(15847..16413)
                     /locus_tag="VFQ61_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953706.1"
                     /translation="MTLSIQFWVATIVTGLACSSTPPQTSTQLSEMGGVTSVSSTAGS
                     PSSGASGATSGAGGATENGVQTQGGVQTQGGAQAQAGVQSQAAPECSTKSDCRLVSDC
                     CRCLAAPKTGQLDACFGGCIQTRCVENQISGSAVDCVSGRCVLGFSCNASRVTCTLPT
                     PECSSGLVPSVNDQGCWGACIASTECRD"
     gene            16811..17572
                     /locus_tag="VFQ61_04370"
     CDS             16811..17572
                     /locus_tag="VFQ61_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953707.1"
                     /translation="MAPAIAGASAAASGGGSEALGSGAAQTSVSGAGQLGSSTGGTLG
                     AGAPAAGGSAGGSGGSPATGAGGTGPSAVPGLSAVPGLGAVGSLSCVPTGHVTYTLVT
                     GSTPTASEQVAFKLIAVAMELAVAYYNCHTPIQRTISVYYDPTFEVIGGGDGIIRFGS
                     IDSMNPVTAMHEIARVLGVGSEAYAARVKNGVFTGENATRELRAITQDPNAVVRATAK
                     TFSPYGLNYLAEVTSLEDLVRHCRMVAAILQDLAG"
     gene            17647..18189
                     /locus_tag="VFQ61_04375"
     CDS             17647..18189
                     /locus_tag="VFQ61_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET9953708.1"
                     /translation="MSKSRQKSSVPAPAAPTDPLDRALDVFRDALHQRGLRYSSVREA
                     IARAAVAYPGQFEVNDLMRELQQAEVKDAHLATIYRTLPLLVETGLIRPALLSSGERH
                     FYEPAFERPHHDHLICSACGKIVEFEFEAFEVLQQDIAKHYGFELMTHVHELIGRCGD
                     CQRLPATSAAVAEARESEPQ"
     gene            18228..>18529
                     /locus_tag="VFQ61_04380"
     CDS             18228..>18529
                     /locus_tag="VFQ61_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953709.1"
                     /translation="MGSSQLPLFGHEAPSFDRSFTGLQHIDLGDGAWLEYRPEWLKGA
                     QTVFESLLTTTRFRETEQWLYERNVKTPRLIASLPEDGPGHPLLFELRDVLSLRYA"
BASE COUNT         3316 a         5824 c         6006 g         3383 t
ORIGIN      
        1 cgaccacggg ccaacgtagg ccgcgcgcct tcacgtattc cgagtagggc gccaggtcct
       61 tgtgcttgtg ccggctgaac ttgcggtact cgttgaacag gtgttcgtcg acgttcacgt
      121 cgtagtagtg ggcgaagtcc cagatgggaa cctctttgcc gtctttctcg atcgcgaata
      181 ggaacttacc gtccttgtcg cgcatacccg cgtggccgcg ttcgtagagc cgccgcgcga
      241 gcgcaatcaa ttgccaacaa tcatcgcgcg cgtcgccggg cggcttcacc attcggaacc
      301 actgttgagt gcgtcgctcc gagttgccga acatgccgtt cttctcgatc cacatggccg
      361 agggcagcac gagatcggcg gcttgcgcgg taacggtcgg ataaacatcc gagacgatca
      421 ggaacttgcc ggggatcttg cggctagcct cgaacagcgc gtggctgttg ggtaaggtct
      481 gtcccggatt cgtcacttga acccacaagg tgtcgatatc accaccttga tcttggggcg
      541 tcgagaagcg cttccacatc tcgaccgcgt ggtagccagg cttgccggag atgcgccctt
      601 ccgggacgtt ccagtactct tccgcgtgct tgcgatgctc tgcgttttcg accacgagcc
      661 cacccggtaa cgcatgcgcc agggtgccga cttcgcgcac cgtcccgcac gcggaaggct
      721 gcccggtgag gctcatcggt gcgtcgccag ggcgaccgaa gtggccgctc aacaaatgaa
      781 ccgcgtgcac caggcagttg atggcggtgc ctcgcgtgtg ttggttcatg cccatgcacc
      841 aagtactggt gatgcgcaga tcccgacgcg cgaatagatc cgcgagatat ctgagatcgg
      901 cctcggaaac ccccgcgagc tcctccactc gtttgggggt gtactcggcc aggccgcgct
      961 tgaactcctg aaagtcgatg ggcttgcccg ccagcgtggg cggatcgctg tcggcccgga
     1021 aagcgacgtg cttggcgacg aactctttgt cgtagcgatc gctggtcagg agcaagttca
     1081 tgatgccgtt ggcgacggcg agatcgccct gcggaatgaa attgatgtgg cgatcggcct
     1141 gggcggtgct gcgcgtcttg cgcgtcgcca gatcgataat caccaccttc tcaccgcgtg
     1201 cgcgccgatc gatcacgcgc gaccacagca ccgggtgcat ttccgctggg ttattgcccc
     1261 acaagatcag cacatcacag cgatccagat cgtcgtaaca gccggcgggt tcgtcgacgc
     1321 cgtaagtcgc caagaagccg gtgaccgcag aagccataca taggcgcgcg ttcggatcga
     1381 tatggttgtt cgacagcccc gccttcatca gcttgttgcc ggcgtaacct tcggggatgg
     1441 tccattgacc gctgccatac agcgcaaacc ccttgggatt cgcgagcacg cgggaagcga
     1501 tgatgtcgag cgcctggtcc caagaaatcg gcgacaactt gccgttctta cggagcaacg
     1561 gctgcgtcag ccggtccttg ccgtacaagg ccaggccaac gtggtagccc ttcatgcaca
     1621 gtagcccacg gttgacttcg gcgcgggcgt ctcccgcgat ggctacgacc tttccgccct
     1681 tcacgcccac ttgcacgtgg cacccagttc cgcaaaaacg gcagggcgcc ttatcccact
     1741 ggacgccgtc gccgttgcgc tcgcgcacgg agtcatccag cgcggggcgg ttgtccttgg
     1801 agccaagctc cttggctcgt atatctccct gcgccatgcg cgtggcggcc gccgtggcgc
     1861 tggctagaac gctgctgcgg atgaacgctc tccgatccat gactcagctg tccctccctg
     1921 cccgcgcctg aacttcggtc gacgttggag ccgcctcgga ctcgcgaatg aggtcgagat
     1981 cgctctggtc gagatgaatc aagtctgcct gcagaactcc aggccaagcc tcgagctgtt
     2041 cgtgacggac gcggctcgcg tccagagtag gcgtttcgac gacgagtggg aggcgtacgc
     2101 cgcacgcctc tccaaaggtg atctcggaat cgtccgcgag cgcgcgacgt agttctccga
     2161 tcgatgacac gtctcgcatg gtgagcacca gtccactgca cggcatcggc tgcggctcag
     2221 caaatagcga gccaaacgcg gcttagccga ctgaagactc ccgagcctct ctttgcgtac
     2281 gggttgtcac cgtgaaaacg agcccgctcg accctccgcg tcggatgact actttaatat
     2341 gcatttcaaa tgctggtttg cgcacgaatt gcgtcagacc tcggattgcc tgcaagtccc
     2401 gcgaatcagc tcgtgcgtca gggcacgtgg gctttgcgtc caccagcgct cggcggagcg
     2461 ggagatcccg cggcttgcgg ctctgctcgg atcgatcttg ggctcgagat cggccttggc
     2521 atacgtcgtg ctgacggctc gccgtgccgt caccgttcgc atgggagttt gcctgctgga
     2581 tgtgcgtgct ggctttgtgg ggctgcaacc gtcccatcaa agaagtgccg agagcacgcg
     2641 caccgagcgc ggctcgttac ccggtagaga tccgtcgacc ggcgaagctc tcgagcatcg
     2701 agaccgggcg agccgacgcg ctcgggcgcg aacagcgcgt gccttgcgtg acgtgccact
     2761 ccttgggggt gcggcgaccc gtgcccgaga gcagcgctga gctcgaagaa tttcaccgtg
     2821 gcctctcgat cgagcacggc tcgctacgtt gcgcctcttg ccattccgcg gagggagctg
     2881 aacttctgaa gctcgcggat gggcgcagct tgcccatcac cgacagcatc gagaagtgtg
     2941 gggagtgcca cggcccgcaa taccgagact accgacatgg agcacacggc ggcatgttgg
     3001 gttattggga tctctcgagg ggtgggcggc tgcgcaatac gtgcgtcgac tgccacgatc
     3061 cgcatatccc caaaccgccc ccggtgatgc cggtgctgcc gccgcgagac cgttcgagta
     3121 cggcccactc cgtacagcct gtcgccgaag cgcgaggtgc cgcccatgac tgaacccaaa
     3181 cgccgtgtgg ccctcaaagt actcggagct accctcggcg ccgccgcgtt cgcgcgcgcc
     3241 ctggcgccgt tgcagagctt cgccaaggcg ctgtcgctcg atgagttcct gcagcagcac
     3301 tatcgagaac tctcgagcga agatctccaa atcatctttc gccggttgga ggcccagacc
     3361 gcgcgcgact acggggccaa cgtccacatc tccgacccgc gccccatcga aggtgtggag
     3421 ttcgcttacg ccttgaacct gagcgtatgc attggttgcc gaaagtgcgt cgaggcttgc
     3481 cacatcgaga acaaccacga tcgagcgagc aacaactcct acatccgagt gctcgagatg
     3541 gagcagggct cgttcgacat ggagaagggc aacgccgcct acgaccaccc cgtgcccgcg
     3601 cccggcaagt actacatgcc ggtccaatgc cagcagtgtg acaacgcccc ctgcgtaaaa
     3661 gtctgccccg tccaggccac gtggaaggag tccgacggta tcgtcgtcgt ggattacaac
     3721 tggtgcatcg gctgccgcta ttgcgaggcg gcctgccctt atcacgcgcg gcggttcaac
     3781 tggacgaagc ccgagattcc gaaagacgag attaacccca accagggtta tctctcgaat
     3841 cggatccgcc cccaaggcgt ggtcgaaaag tgcacattct gtctgcatcg cacgcgcaat
     3901 gggcgcctgc ccgcgtgtct cgaagcttgc cccaccggcg cgcgcgtgtt cggcaacttg
     3961 aaggacccga acagcgagat ccgttgggtg ctgaacaaca agcgcgtgtt cgtcttgaaa
     4021 gaggagctcg ggactttgcc gcgcttctac tacttcttcg acaagtaacc ggctcactct
     4081 cctgcagcgg ctcacgacac tatggcccag ctaacctctg gtactcccac gccgagcccg
     4141 gcgcctctcc aatcactccc gcacggtgag ccccacctca cgagctaccc tcgtttcctc
     4201 tggcacctac tccagggcag ctttcaaggc tcacgcttct tctatgtctg gatgacggcg
     4261 ttgaccgccg tggcgctggt cggcgcgaac gcgtgggcca aacaagtagc gatgggcatg
     4321 ggcgtcaccc acatgtccga tcacgtgtct tgggggctct atatcgcgaa cttcacgttt
     4381 ggcgtagggc tcgcggctgg cgccgtgatg atggtgatcc ccgcgtacct gtacgaagac
     4441 cacgaaatgc acgagatcgt gctgatcggc gagctcctcg cgatcgcctc gatcgtcgtt
     4501 tgcttactgt tcatcacggt cgacctcggg cgccccgatc gcttttggca catcgtgcct
     4561 gggctaggac gcttcaattg gcccatatcc atgctcacct gggacgtgat tgtgctcaac
     4621 ggctacctgc tgttgaacct gcacatcacc gggtacctga tgtacatgcg cttcttgcgt
     4681 cgcgagccga acccgcgttg gtacatcccg ttcgtgttcc tatcgatggc gtgggccatc
     4741 tccattcata gtgtcaccgc gttcttgtat agcgggctcg gcgggcgtcc gttctggaac
     4801 tcggcgttgc tggccccgcg cttcatcgcc acggccttcg ttacgggtcc cgcgttcgtc
     4861 atcgtgctgc tccagctgct ccagcgcctg ggtcaactca aggtgggggc ggggccaatc
     4921 cgcacgctct cgtcagtgat gcgcattacg gttctcgtga acctgttcat gctctcctcc
     4981 gagctcttca ccagcctgta caccggcggc gcgcacgccg cgtcggttcg ttacttgttc
     5041 ttcggtttgc atggacatca cgccttggtt ccatggattt ggagcgcgct cgccctgaac
     5101 ctcggctccg tggtgctgct cttcgtgcag cgcgtcaccg ggcgaagcct cttcctcacc
     5161 ctggcgtgta tcgcagcctt ctcgggcgtc tggatcgaga agggcatggg gctcatcgtg
     5221 ccggccttca tcccgagcac gctgcacgag atcgtcgaat acacgcccag tctgaccgaa
     5281 tggaaggtca cgattgggat ctgggcggca ggtctgctgg tcctaacggt gcttctcaaa
     5341 ttggcattgc cagtgctgcg ccgaagcctg gactccgcct cccaagctcc gcagaagcat
     5401 tgacgcagtc tgaatcgcgt accgcgtagc gcgtctcacc cgacaaattc gtcggaacac
     5461 ccggctttgg ggcgcccccc cgtctcggca acccagacgt gagggcaagc ggagcagcag
     5521 ggcgaggcag gtttgcttcc gggacgtccg ccgacaaatc gcaaataccg tattacttcg
     5581 cgctgcaagc tggtgtaggc gtgccgctcg atgcaaaagc tcgtcaaggg tatccactcc
     5641 ttccagaagg gttacttcgc tactcaccga cagcttttcg agcaactcgc tcaggcgggg
     5701 cagcgccccg agaccctgtt catcacctgc tcggattccc gcgtcgttcc caacctgatt
     5761 atggacgcgg cccccggtga actcttcatc gttcgcaacg tgggcaacgt cgtcccgcgc
     5821 gcggatttgc ccgggggtac cgcggctgcg attcagtacg cggtcgaggt cctcaacgtc
     5881 gaaaacatca tcgtttgcgg tcacacccag tgcggcgcca tgaacgccgt gctgcgtccg
     5941 gaaacgatgg aaagtttgcc gttcgtcaaa cgctggttgg cccagacttc caacgtcaaa
     6001 caggtgatcg aggaacgcta cggacacctc gacgcggagg cgcgggtcac ggcggcggcc
     6061 caagagaacg tgctcgccca gctcgagagc ttgcgcgagt atcccttcat tcgggatcgt
     6121 ctggatgaag gcaagctcct ggtcagcggc tggatgttcg acatcggccg cggcaagatc
     6181 ttcgacttcg atccggatca gagcgagttc gtcccgctgc tcgcggactg atgagccgct
     6241 cggtcgacct ttggaatggg cggccgcgga agaacgagcg cgaagcgcgc gccccggcgc
     6301 gcgaaagcgc gctcgtggtg ctcattgcgc gtcgaaacac cgcgcaggcc acccgcaaga
     6361 accccgacgt cggcataacc ccgacgtcgg aggccccgcc gtcttaacac cgagggccgg
     6421 cgcggttcct cgatggcccg cgtcggctct acggctgcgc caggtcgggc ccgcgccgcg
     6481 cgtcgcgcac gtagtcccgt ggccacgccc cacgctactc ctagaagtgc aaacgtcgca
     6541 gcgtgggcgt cttcttgttc tgatgttcgc tcatccgggt gaggacgtcc ttcaagactt
     6601 cgacggcctc ggcgacgtac ggccctttgt tgagcatcac gcattcggcg cgctccgcca
     6661 tcgccgcgtc ggtcatttct gcgcgcgtgg gtaggccctt cttcacgagg tgttcgagca
     6721 cctgagtcgc ccaaaccacg ggaacgtgcg ccgcctcgca aatccagaga atttcctcct
     6781 gaatttcggc taaccgttga tagccgatct caaccgcgag gtcgccacgc gcgatcatca
     6841 ctcccgtcac ggcttggctc gcggaagcga ccacgatttc gggcagattc ctgaccgcgc
     6901 gcgcggtctc gatcttgagg atcagaggaa tgcgcgggtt cttggcgcgg ctcgtcaagg
     6961 tttcgcgcag ggcctccacg tcggatggag actgtacgaa ggaatagccg atgatgtcgg
     7021 cgtggtcggc gacgaactcg agatcgccca agtccttcgc ggtgagggcc cccagcgcga
     7081 gctcgatgtc gggcgcgttg aggctctttc cagccgcgag cctctgcccc ttgtagcgag
     7141 cgcggatcac ccgcaacgag taaccctccc gctgaacggc ttcgacggcg gcgtgcagct
     7201 taccgtcgtc aatcaggagc gaatctccaa ctttcaagtc gcgtagtgcg gcgggctcgg
     7261 acggttccag tacggcgcgc ggcgcgggat cgctcggcgc acggcaatcg accccgcggg
     7321 cgagccacag ccgatcaccc gtatgcgcgc gcacacgatc cggcgtgcag cgcgcgatgc
     7381 ggagtttggg ccctgccagg tccatcatga cgcgcaggga gcgctgactg ctcttgcgag
     7441 cggcgtggac gtgctcgatc atctggcccc actcgcgcgg tgaatcgtga gcacagttga
     7501 tacggaagca atctacaccg cgcgccacca gtcccgcgat gaacgatggc tcctcggcgg
     7561 cctcgctcgg taacgtgacc aagattcgcc cgcgtcggcc gcgctcaccg agcccaaaca
     7621 atgctcgcgc gttgcgttcg agcaaacgct cgcccagata gaagcgcgcc acgctcggac
     7681 gtttcggggc gtggccttgg ggcacattca atgttgccgc gagcgtagcg gtgacggaat
     7741 cgagattggc caagacccgc gcttcgccac gcccgagcga agagaggccg aggggcggaa
     7801 gtagcgcctg cagcgcgcgc aagtcacgac gacgaaggat gagatagtgg gccaagttga
     7861 gcgcactcgg ttgaaacgcg aggcgctcga ttcgagggcg ccagcgcgcg aatagctcgc
     7921 ttccgagcga ctctacttca gcccgcaatt cgaggagtaa agcgtagact cgtcgcggat
     7981 cggcttcggt aggagtaatc gaacccatgc tgttcaccct gtactggtcg agagctgtag
     8041 ggatcctcgt cagggctccc cgatggcgga gctatttgcg aggctcagct gtcgcgagct
     8101 gtgcaaggcc tctgccacgg caagccgtcg cagatccgtc gtgggatcgt ctcggattcg
     8161 tgccggttcg tttcccgttc gtcgcgtcca gcgtgagaga acgtgggaag ggctcgagct
     8221 ggagcctgcg cagaagcacc aggcgtgggc gcggcgcgca tggcttgcgc caacgccgga
     8281 gcgtccgcga aaattaagtc gtcagactga ccgcgggggg gaccaagccg cgcaaggcgt
     8341 ggagtgcgcg accacgttca cgcgcgggca ctccgaagct accgagttgt tgcgctgcgt
     8401 gcgcctgcgg cggcgttcgg ggtgaacggg cttgggtctg ctcgtcgctg gtgttttggg
     8461 cgtgcgcgtg caggttgtgc gcgacttttt cggccaaatc gaggcgcagc gcgcgatctc
     8521 cgagcaccac gtagccgagc gctagccatt ggggcgcgtg acgggctgac gccacgtaac
     8581 tggggcgtcc cagagtgtcc ggcagccagc tgccaggggc ggcgatctcc ggggtctggg
     8641 cggagagtag agcacggcgc tcgacatagc gcgccggcgt aaagcctccg ctgatccaaa
     8701 ccgcgagttg gccgaatcgc agaccatgac ggtcgagccc ggtggtcttc agacccgccc
     8761 tgagctgcgg gatggggaga ccgctgttct tcaataacac cactccaagc ccggtgtgga
     8821 gttgatagta gagtgcgcgc aacaagcggt cttgatgatc ggcgagcgcg tgggcttccc
     8881 ccaccaaacg cgacgaaacg tcgcgcacat aggcgatcag ccggcgttgc agctgcgcgc
     8941 gcaggccgcc gccgatttgg ggcaactcga tcaagcgcac gtcgggcaag gcgagggagc
     9001 tgccacgggt gaggcgagcc aactcggtct gtccggccca aatgcgaccg cgctcgtcga
     9061 gccgaaaggc ttcgtggggc gcgtccacca acggaagtag ggtcgaagac agcagctccg
     9121 tgggcgagag ttggggttcg acttcgacgg ggccgagctg agctctcaat cccgagaggg
     9181 ccgcgaaagg gcggaacgcc aactccccat tggattcgtc gaccgtgggt ggcgcgttgc
     9241 cattcagggt cgagcctgga actccggttc gaggggtacc ctttagtgtg cggcgaatct
     9301 tgttcgagcc ccggcgcgcg acgaagcgat cgaccagggc tgcatggagc gcgtcgctca
     9361 gacggtcttc caggttggcg agcgcagctt ggaacatgct cgcatcacgc acgaactcgg
     9421 actgatgggc gacgaaggcc caagtccgga ggcgcgcaat gcgagccatg agcgcgtcgg
     9481 gatcgccatc gaagcgctcc agctcttcga tttgcgcgta catgaagtct cgtggcaaag
     9541 ggccttcgca gagccgcacg aatagtcctt tgagcaagtc gatatgcacc tcgaacagca
     9601 ggttgcgaaa gtccggcacc tggcagacgt gccagaggag ctcgactcga cctttggcgg
     9661 ccagcagcgg ggtcaactcg ggatcttgca ccagcgcctc gagcacgcgt agatcttcgg
     9721 cgtggggggc gctcgtaaag cacgagagct tgggcggcgc cttcagcgac gcgagcaatt
     9781 cggcggcgga cgagaagtcc aagtcgtgat tgcggtagcg cagggaacgc accggctcga
     9841 agctttgagc ctccaagctg cgggccacgt cgagcggcaa ctcgagcggg gcgatggtgc
     9901 cgaaggtccc gtcttgcaag tagcgcccgg cgcgccctgc gatttgcgcc agctccgcgg
     9961 ggctcagcgc gcggggtcga tgcccatcga acttgcgcag tgaggcaaat gccacatgtt
    10021 ccaccgccag gttcaagccc atcccgatgg cgtcggtcgc cactagatag tcgacctcac
    10081 cggattggaa catcgcgact tgagcgttgc gggcacgcgg cgaaagtgca ccgagcacca
    10141 gggccgtgcc gccgcgcaac gcgcagagcc gccccgcgag ttcgtagagc tcgcgcatcg
    10201 agaatccaac gagcgccgtg cgtggtttca gtcgcgagag cttcgtcgtg cccgcgaagc
    10261 tcaagcgtga gaggcgaggg tgttcctgca gccgggctgc gggaacgatt tgcgagagga
    10321 cgccccgcat cgtggatgag ccgagaaacc aggtttcttt gcgcccgcgc gcgtgcaaca
    10381 gccgatcgct gaagacgtgt ccgcgttcgt cgtgtgtggc gagctgcacc tcgtccaccg
    10441 ccacgaagtc cacgtctcgc gtgagcggca tggcctcggt cgtgcatacc caatacgacg
    10501 ggcgacgtgg cagtcgcttt tcttcccccg tcacgagtgc cacacgatct tctcccaaac
    10561 gcccggcgat acggtcatac acctcgcggg ccaggagccg caaaggtaag ccgatcatgc
    10621 ccgagtcgtg gctgagcatt cgctccacgg ctcgatgcgt ctttccggta ttggtcggac
    10681 ccagcagcaa ggtactctgt gtcgcctcgg agaccggcac ccggccagca tgacgcgcct
    10741 cgggcggggg tgctagatca gtttcggaaa gggcgccgcg tgctagccat gaacctcagc
    10801 gagctcacac aggctttcga acgtcgtttc gctagtagcc cgcggctttt cgcagcccct
    10861 gggcgcgtca acctgatcgg tgagcatacg gactacaacg acgggttcgt gctaccgatg
    10921 gcgctcgagc tggcgacggt ggtcgcggcg agcccgcgta gcgatggggt tttgcgcgtt
    10981 tacgcgcaga acttcgatga gactcgggaa gtgtcgttgc gggtcgagtc ggcagcgggg
    11041 gcgcgcagtg gccactggct cgactacgtc gagggtacgg cgcgcgtatt gctgcggcag
    11101 ggggtcgagg cgtcgggcgc agatctctgg attcgaagcg agctgccgct cggtgcgggc
    11161 ttgtcttcgt cggctgcgct cgagctcgcc gtcgggctcg cgctcacgag tttgaccgat
    11221 cagcctcgtg atttgcagag cctggcgttg gcggggcagc gcgcggagca cgagtacgtg
    11281 ggcactcaat gcggcatcat ggaccagctg tgtgtcgcgc tcgcgcagcc cggatatgcg
    11341 ctgttcatcg actgtcggtc gctcgcggtg cagccggttc cgttggcttg gcgcacggcc
    11401 gaagggctcc gagaagtctc ggtcgtgatt tgcgactcgg gggtcaagca cagcctggcg
    11461 agctcggctt acaatcaacg tcgcgccgag tgcgagagcg cggttcgggt gttgcgccag
    11521 cacgcgcccg agattcgtgc cctgagggat gtgagccgcg agttgctcga gcgtcacgcg
    11581 acggagcttt cgccgacgga gctcgcgcga gcccggcacg tcgttacgga gaacgcgcgc
    11641 acggaggcgg cgcgggaagc cttattgcgt ggcgagctcg cgctggttgg acagctgatg
    11701 tacgagtccc atgcctcgtt gcgcgatgac taccaggtta gtgttcctga gctcgacggc
    11761 ttggtcgaga ttgctagtcg agtttccggc gtgtatggct cgaggatgac ggggggtggc
    11821 ttcggcggat gcaccgtcag cttgtgcgag ccgagcgttg tctccgagct cgaagcggcg
    11881 ttcacgcgcg agtactcgaa gcgcttcagc attgctcctc gcaccttcgt gagccgcgcg
    11941 ggtgcaggcg tagctgaagt ctttcctccg ggtgtgcctg cgggcggacg cccctgagtt
    12001 cctcccggtt ttgggaagac agctgcgaaa ggccgaagtc gcgcagacgt tggctgagcc
    12061 tcggggggcc gctcgagtca ctgtatcgtt cgacatgtcc aagggtctgt tcgcttcatt
    12121 ggtgctgctc gcgtgctcga gctcgaacga aaatgcgggc ccggggcaga accccgggct
    12181 tgggggagta gcagggctcg gtgaagggac ggtcagcggg gccggtgggc caagcaccaa
    12241 cggcggcagc tcgggttcgg ctggtgcgtc gtcgcccaac gcaggagggc tcggcggtat
    12301 gggcgagttc ccggcaaagg gcgcggctcc aagcggcggt gccggttccg gtggcactca
    12361 agcacaggtc agctctggcg gtcgcagctc gggtggacgg agctccgggg gccgcaattc
    12421 gggcggaggc gagtccggag ctgggccggt cgccggcgca tcgaatggag gcgcggcggg
    12481 tggtgcaccg gcgagcggcg gagcgggatc gaccagcgct ggtgctggca cttgtgtcga
    12541 gagcaattgc ggaacgcaca agtgggcgtg ctggaagatg ccgaatcccg cgagcagccc
    12601 cgcctccgtt cccaatcacc aaagtcatac cgacctcggc aacggagccg ttcgcgacaa
    12661 catcacgtgc ctcgtgtggg agaaggccaa tccagacaag aagggtaatt ggcaatctgc
    12721 atccgatcgg tgcgcggcgc tcgcgagcag tggttacgcc ggattcgatg attggcgctt
    12781 gcctacgcgc gtggagatgg cgtccatttc ggacgtgacg ctcggagcga agggatatcc
    12841 ggcgccattc ggggtgacca gcggctacta cgtgaccggc tccttctggt acaagactat
    12901 cctcactgcc gggggtgcca aacccgaaga tcgcgtttgg ggctacggga ccaacgggtt
    12961 cacgagcaac gccatcgtcc gctccaacac cgataacctc gcgcgctgcg tgcgcggcaa
    13021 tggtcccggc gaggccgcgg gcgactacgc cgtcgagccc cccaatcatt acacgctcgc
    13081 gggcagcggc gtcgacgcga cggttaccga caactacacg gggctcacct ggcaagccac
    13141 ctactccaaa gcgcgcatgc cctggtccga agcatcggct tactgcgccg cccaacgaac
    13201 cgccggtctc tcggattggc gcgtttcaac cctcaatgag ctggcgagca cggtgaacga
    13261 ggccctcgtc gcgcccgcca tcaatcgcac cgtgttcccg aacacgagcg gggcttgcga
    13321 tgagaccggc tggttttggg cagccgagtc ttccaaagta ggcggcaccg cctggggcct
    13381 gagctactgc gacggctaca ccggctggaa cgctggcgcg agtgcatccg cttggaacta
    13441 cttcacggac gcttacgtcc gctgtgttcg atgaccacgc gcggtgggct ctagctactt
    13501 ctagagcaag ggtgctgctt ggggcgtact cacgggttcg cgggcgctct tcgagcgcac
    13561 gaccagtaag gacgtttggc actgatcgag cagtcgctcc cgctgaactc cgaatgcgcg
    13621 ttcctcgagt ccgtagctgc ggctcaggcc gatgatgacg aggtcgtagt cgttccgcgt
    13681 ctccttcagg atggcggacg cgggatcggg cgcggcttcg acccgaaagc gtacgcggct
    13741 accccgtcct tcggggaaga cgctgtctac ggcctcgcga gctcccaagg gttcacgcgc
    13801 ggcttcggcc tgatccggcg cccgttcgat cacgtgtagg atcacgactt cactcccggg
    13861 tttgctcaag atgcgccggg cgatggcgag cgcggtcaag tcgtgcgggg tgcccatgaa
    13921 gggtaccagc acgcggcgca cgtctttcag accacgatcg atgaggaccc cgacgtccgt
    13981 gcgcgccagc cgcatcacgc ggtagacact gccgcccagc aagctctgcc ccagaacggg
    14041 tttgtgactg cccagcagca ctaggtcggc ttctttgact tctgcgacgt tcgcgatatc
    14101 ggccgccgga tcgattgaag tgaagccgag cgaacggatc gggaggttcg agctccgcgc
    14161 gcgttccaaa agcggtttga gcgtcgcgtc cgcgagcgga cccgcctcgt ccgcgaagga
    14221 gccccgttcg ctcggtgtga tcaggtgtaa ggcgtaaact cgggcgtcgc gcgctggaca
    14281 caaggcatcc gcaagcgacg ccaagctggg ccccacccgc gagtcggcca cgcaaatcag
    14341 cgcggtgaag ccgtcgttca cgataggaac cgcggactcg ggctcggcga tcgtctcttg
    14401 caagcgcgac cgcggataca atcggtgcaa cagcggagtg gtcaaagagg tagtcaccaa
    14461 cgccattacg accatcatcg tgaagagctc tggcccaatc acgccgagat ctaggccgat
    14521 gttgaggacg attagctcca tcagtccgcg cgtgttcatc agggccccca gtgcgctcga
    14581 ttcccgccac gagagccccg agatgcgcgc ggccagcgtg ctcgctccga acttcccgat
    14641 gcacgcgacc gcgacgatgg ccaagcacat caaccaggcc gccggtgatt gcaagagacc
    14701 caggctcgtt cgcagaccgc tgaatgcaaa gaatagcggc agcagcagca ccaacacgag
    14761 gtcttcgagc ttgtccgcca gggcgcgcac gtagttaccc tgtttgggta cgatcgcgcc
    14821 aaataggaag gcgccgaaca gcgcgtggat gccgatgctc tcggttacga agctggagac
    14881 caggagcacg agcaacgtcg cggcgacgac gttctgggtc aagacctcgc gtccggaaat
    14941 ccgtatcgcc agccgtgcga gggtgcgccg cacgaccagg aacatcaggg cgatgtaacc
    15001 ggctgcgagc agggtggtga cgagcgccga gcccagcccg gaagctcgac tgcttgccac
    15061 cacgaaagcc aagatgcacc acgccgttac gtcgtccacc gccgcgcagg tgatggtcac
    15121 gttgcctacg cgcgtgttca acaggcgctg ctccttgagg atgcgcgcga gcaccggaaa
    15181 cgcggtgatg ctcatcgcgg tgcccatgaa caacgcgaaa gacatgaacg gcacgccctc
    15241 gggccgcagc tgtgcctgca ggccaaacgc gagcaacaca cccaacacga atggcgcgac
    15301 gatgctggcg tgcgaaatag cgatcgatct acccgagtta cccctcaaca tggccggatc
    15361 gagctcgagc ccgaccagga acatgaatag aatgagtccg agctggctga tcgcgccgag
    15421 cgcaggcaag gatgccggct cgaacacgaa ccgataggcg tttggccaca gccaaccgaa
    15481 cagtgaaggc cccagcagaa tcccggcggt gatttctgcg agcacgcggg gctggtgcaa
    15541 gcgctccgtg agcctgccga gcacgcggga actgccgacg atcagaccaa tctgaagcaa
    15601 cagccgcggc agcggagcat cgagcaggcc aggatggctc atgggtggcc gagacgtgag
    15661 aggcggatcg gtagactcgc tgagtgcgcc gcccagcagc gcgtacggtc ctcgtagcgg
    15721 aggtcacgcg gggggtcgac gcaaaagtat ggcgggcgcc tcaagccgca agttcagcga
    15781 ctggtttcag tgactggagg tcggccgttc ggggaggttg ctgcggcgtc gagatcgatt
    15841 gcggcgttaa tctctacatt cggtcgaggc gatgcacgcg ccccagcaac cctggtcatt
    15901 gacgctcgga accaaaccac tgctgcactc gggcgtcggg agtgtgcacg tcacacggct
    15961 cgcgttgcag ctgaacccca ggacgcaacg ccccgagaca cagtccaccg cggagccgct
    16021 gatctggttc tccacacatc gagtttggat acagccgccg aaacaggcat cgagttggcc
    16081 tgtcttgggt gcagccaagc accggcaaca gtcgctgacg agccggcaat ccgatttggt
    16141 gctgcactcg ggtgctgctt ggctctgtac ccccgcttgc gcttgtgccc cgccttgagt
    16201 ctgtaccccg ccttgagtct gtaccccgtt ttcagttgcg ccgcctgccc cggaggttgc
    16261 tccggaggcg ccggaggagg ggctcccggc ggtggaggac accgaggtca ctccgcccat
    16321 ctcggatagt tgagtggacg tttgtggagg cgtactgctg cacgccaaac cggtgacgat
    16381 agtcgccacc caaaattgga tagagagagt cattcggcac acgcgagcag caaacaaccg
    16441 gagtttgctc ctcaaacgga aagacttcca ggttcattgt tgcgacgagg tttgcgcgcc
    16501 ccggaaacat cagcctgcaa aaaggctcat caaacagcga tggggaaaag ttcgggttcg
    16561 actcgaatgg ttgtgattcg agtccaggaa cgacggtttt tgacgagagc agccgacaag
    16621 ttggtgaaaa aagcgattgg tcatatgcat gctggcgagc acgcgagaac tacgcaatac
    16681 ctgagtcgcc atgtttgcgt ttcggcacga cagcagaagg ggagtgttgc gcgggttcga
    16741 ggtgagtgcc gcgctgctaa tgggacttgc cgcgtgctcg ggctcccccc aatcatcgac
    16801 tgacgcaaca atggctcccg cgattgccgg cgcttcggcg gcggcgtcag gaggcgggtc
    16861 ggaagcactc ggaagcggag ccgcacaaac gagtgtgagc ggagcaggtc agctgggaag
    16921 cagcacgggc ggcacgcttg gcgctggcgc gccagccgcg ggaggctcgg cgggtggtag
    16981 cgggggctca cccgcgacgg gcgcgggcgg taccggtccg agtgccgttc cggggctgag
    17041 tgccgttccg ggtctgggcg ccgtgggctc gttgagctgc gtacccactg ggcacgttac
    17101 ttatacgctc gtgaccggat cgactccgac cgcgtccgag caggtagcgt tcaagctgat
    17161 tgctgtcgcc atggagctgg cagtggccta ctacaattgc cacacgccga ttcaaaggac
    17221 catttcggtc tactacgacc ccaccttcga ggtgatcggc gggggcgacg gcatcattcg
    17281 cttcggctcg atcgactcca tgaaccctgt cactgccatg catgagatcg ctcgtgtgct
    17341 gggggttggc agcgaagcct atgcggcgcg cgtgaagaat ggcgtgttca ccggagaaaa
    17401 cgcgacgcgc gagctgcgag ccatcacgca ggatcccaac gcggtggtga gggcgaccgc
    17461 taagacattc tctccgtacg gcctgaatta cctcgcggaa gtgacctctt tggaggatct
    17521 cgtgcgccat tgccgaatgg ttgcggccat cctccaagac ctcgcgggtt gagccgtcat
    17581 tggcgtcccg gggggcggat cccgcccttg acggatggcg cgctcttgcc aagagtcatc
    17641 cagccgatgt ccaagtcccg tcagaagtcg tctgtaccgg cgcctgccgc gccgacggat
    17701 cccctcgatc gcgctctcga tgtgtttagg gacgccctgc accaacgcgg cctgcgctac
    17761 tcctcggttc gagaggcgat cgcccgtgcc gccgtcgcct acccggggca attcgaggtg
    17821 aacgatctga tgcgcgagct gcagcaagcg gaagtcaagg acgcgcacct cgcgaccatc
    17881 tatcgaaccc tgcccctgct cgtggagacc ggcctgattc gccccgcgct gctgtcatcc
    17941 ggcgagcggc atttctacga acccgcgttc gaacggcctc atcacgacca cctgatttgc
    18001 agcgcgtgcg gcaagatcgt cgagttcgag ttcgaggcgt tcgaggtgct gcagcaggat
    18061 atcgcaaagc actacggctt cgagctgatg acgcatgttc acgagttgat cggtcgctgt
    18121 ggcgattgtc aacgcttgcc ggcaacgagc gccgctgtag ccgaagctcg cgagtcagag
    18181 ccgcagtagc gcatgcatcc ggcgcgccca gcgctatctt tcacggcgtg ggctcgtcgc
    18241 agctgccgct gtttggccat gaggctccca gcttcgaccg ctctttcact gggctgcagc
    18301 acatagattt aggcgacggc gcttggctcg agtaccgccc ggagtggctc aaaggggcgc
    18361 agaccgtatt cgaatcgctg ctcaccacca ctcgctttcg tgaaacggag cagtggctct
    18421 atgaacgcaa cgtgaagacg ccccgcctga tcgcgagcct gccggaggac ggcccgggtc
    18481 acccgctcct cttcgagctg cgcgacgtcc tctctttacg ttatgcgga
//
LOCUS       DASTKU010000122        16281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_23524,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000122 DASTKU010000000
VERSION     DASTKU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16281
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23524"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(493..2124)
                     /locus_tag="VFQ61_04385"
     CDS             complement(493..2124)
                     /locus_tag="VFQ61_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953710.1"
                     /translation="MNTETESSASRLARWSSIGALAGDRVWRAIERAVRSRRYVLMCL
                     VSMVLVRIAWVAWIEVQPTSDFTWYYSQGLRIASGQGYTVEHTGFPLWESGTPLPTPR
                     KTAFWPVGYSGFLGVLFYVSQGWLDRLGSPLVIAKLANVALSVITVWGTARLSRLWFK
                     SELTERLTLLVAGFAPNQIAYVALTACETYYTALLVLGVLALAHAAQPDTRMPTGALP
                     GNIRSLGWALASGVLFGLATLTKPQTSLLPAALLLIWAPWRQLYASLSRESGAGSVRE
                     DCALEATSPPPLSKHTPAEISSLGRSSRASEQWTWSARPIRLWLSAGPWLKKAACVYV
                     PLCLVVLPWSVRNYLAFGEWVAVSTNGWMNLAIGNMPGSWGNQGLMWNRELHEVVQRY
                     SDELSWNAGAKSVFLHYVQQHPLQVIAGIPGKLIALYTWDVDGFGWARLGDLRKTGES
                     YYLPLRVLSQMNYLAAFALAAYAVLHLRRALTPGIRAALVVIAYFTVLYTIFFGGPRF
                     HFPVMPFIYMFAAAGLARLISRPGTLERAPTAAAP"
     gene            complement(2459..3277)
                     /locus_tag="VFQ61_04390"
     CDS             complement(2459..3277)
                     /locus_tag="VFQ61_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9953711.1"
                     /translation="MTSLPNLAERPVIVEANLETAPWLAWALGASIAFHALLYGALGD
                     SNAHAARRELKSTVSFELASPPAPAIPEPPQPASTPVPESNPNPARSRPSVTTRVPSA
                     PPPAENPPPPNAARTVDLTGITLAGDSGSFDAPLGNGESRTGPLAAIHPGRSPNPAPQ
                     AAELPAHTSEPKLVAMADLSAKPAAPELGAALRSNYPAEARRRGIAGTASVRARIDSD
                     GVVRQVNVLGESFTGFAEACRRTLKGSRWSAPRDHQGQRVATEIKYTCRFLVTE"
     gene            complement(3252..3674)
                     /locus_tag="VFQ61_04395"
     CDS             complement(3252..3674)
                     /locus_tag="VFQ61_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9953712.1"
                     /translation="MAQANSSQDEAITSINVTPLVDVVLVLLVVLMVTASYIATRSIP
                     LNLPKGSTGESAQAPLTVSLDPSGQLYVDGQHATEDELRGRIQRLRAANATPKAMIAA
                     DGAVPHRNVVALLDLLRQEKVTQFAINVSPDDLAAKPR"
     gene            complement(3684..4316)
                     /locus_tag="VFQ61_04400"
     CDS             complement(3684..4316)
                     /locus_tag="VFQ61_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9953713.1"
                     /translation="MNLVNRLSSLTAHGATWVLWVLVALSVAALAVVIERAVLFATSK
                     DDVHRLRGDLRRLLGRGELDEARRKLQESPSFEARIAVRGLDSETVESASEHMRGETE
                     LARLAMERNLTLLGTLGNNAPFIGLLGTVIGIVRAFRELEGAGGQVSSGLMSEIGEAL
                     VATAVGLMVALPAVAFFNLYQRVIRARLGRAEALISEVMAFRKSPAKAAE"
     gene            complement(4467..5390)
                     /locus_tag="VFQ61_04405"
     CDS             complement(4467..5390)
                     /locus_tag="VFQ61_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953714.1"
                     /translation="MKRSSIGLLGFGYVLGISCLSFGCDDPIERAQDITRSRVLAARS
                     EASQEPSRATPRPGEAVSVRWLVASPDPQPRIGAGFSACMGADWNSGTGDCLEAPFAL
                     ANRSEVEGSELEFTFELPETWDAVAHPRIHLEGTFCAGSEATESGACTGGQTGLRAVF
                     DLNLETPEFTNQNPAFEEPAILLDGAPWLAAPEPFDCGSALVPQLAADATIHELEIRI
                     DAGSREPVSPVLAEDPTRESILVSHFASAGSLDRPFSRLSWSEENTGVRVPWSAPATV
                     EAPGTRAHFWIVLRDERGGTAYIERSVCLIP"
     gene            complement(5387..7843)
                     /locus_tag="VFQ61_04410"
     CDS             complement(5387..7843)
                     /locus_tag="VFQ61_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9953715.1"
                     /translation="MLRKSRWFAALLGLAACSASLHSAAQAPDSTAAIEPPRLSEGVR
                     PVAPHSSSNSSPPRVLLELIILEDGSVGGVEAVQSSDATLEPLALEAARQLKFTPARD
                     AGTPVRVKIRYLLEFAPAAVAPEAAAAEAGTRPVAGERSSHGPGQSTAVSSPNSPSAA
                     APSNAAPMTNANATAKDELPEFEATASVEAPPSEVVRRTVDETTIQRAPGTRGDALRA
                     IELMPGVARTSLDSGAPLIRGANFNESATLLNGTRVPFLYHFGGLTSFLSSRMVSRID
                     FYPGNFSVRYGRIAGGVVEVRPRDPDASKLRAALDLNLIDSSAFVEAPLGERTHVAAA
                     VRRSNIDFVFENFVPKDAYSVVAAPVYFDYQLFGVHRFDSHTKLRVLGYGSRDTLELL
                     FSNPTDFDPALAGSIRGKIEFHRLGLELEAQPSQAVQSHFSLTVGQLGIEQYIGDLRQ
                     VLRGPELYARAETRLELDPRLRLTLGGDLASTIVTGEYRGPYPGQFEGDPSGQDALNG
                     QRTITAKLPATTLIKPAGYVELAYRPVPELFLIPGVRVDYLGESKSLQVDPRFALRWE
                     VTPKTAIKAGVGRFSQELEFWQRLREVGNPKLEPYRTIQASAGFEQRLGARAQLGAEA
                     FYKRIQNGVSATPGHALPHFQNTGTGNIYGAELSAQARTPSNAFLYLAYTLSRSERRD
                     RPGAPMRLFDRDQTHILSLVASQPLGKGWELGARFRYVTGNPTTPVKGSVFDARSGSY
                     LPIYGDTNSDRTPAFHQLDVRLEKLWHAGPVKLAAYLDVLNVYNAQHPEGTRYNFDYS
                     KSESVTGMPIFPNLGLRGEL"
     gene            complement(8019..9404)
                     /locus_tag="VFQ61_04415"
     CDS             complement(8019..9404)
                     /locus_tag="VFQ61_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HET9953716.1"
                     /translation="MQNVPTNPRETGNTDVLSLIGNTPLVRLTSLDTGPCELYVKLEN
                     QNPGGSIKDRIGLSMIEAAEREGKLGEGAMLIEATAGNTGLSLALVAARKGYRLLLVI
                     PDKMSQEKIFHLRAMGAEVRMTRSDVGKGHPEYYQDLAERLAREHSAYYVNQFNNPAN
                     PLAHERTTGPEIQRQMQGRVDAVICGVGSGGTITGLSRFFAKASPATEIVLADPVGSV
                     LSDYVKTGKIGNAGSWLVEGVGEDFVPSICDLSRVREAYSVSDEESFLTARALLRREG
                     ILGGSSTGLLLAAALRYCQAQTEPKRVVTFVCDSGNKYLSKMYNDFWMRDQGFLRGPD
                     QGDLRDLIARKHEQVISLTPDDTLLVAYSRMKLYDVSQLPVLEAERIVGIIDESDLLL
                     AVHDDESAFRRSVRSTMSTKLVTVQVDRPLAEVLPLFDRGLVPIICEGSTFLGLITRI
                     DVLNYLRRKLR"
     gene            complement(9446..10597)
                     /locus_tag="VFQ61_04420"
     CDS             complement(9446..10597)
                     /locus_tag="VFQ61_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HET9953717.1"
                     /translation="MRSPALETLLIHAGQSPDPVHGAVMPTLVLSTTFAQPEPGQPLR
                     FDYSRSGNPTRATLEACLAALEKGKHGFAFASGCAAATTLLHSLSPGDHVVLSDDVYG
                     GTYRLFQRVMSGFGIQASFVDATDPTRVAAAFTDRTKLVWLESPTNPLLKLSDIAAIA
                     RLCRERGVRLLVDNTFASPALQTPLELGADIVLHSTTKYLNGHSDVVGGALVTNDDAM
                     AERLRFLQNALGGVPSPFDCYLLLRGIKTLAVRMQYAVASAQKLAERLAEHASVERVH
                     YPGLASHPQHELACRQMRGPGAIVSLVLKAGEPAARSFLKSLRWFALAESLGGVESLA
                     EHPALMTHASIPRETREKIGIADGLVRLSVGIEHVEDLWADLEQALGAS"
     gene            complement(10765..12099)
                     /locus_tag="VFQ61_04425"
     CDS             complement(10765..12099)
                     /locus_tag="VFQ61_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET9953718.1"
                     /translation="MGFCASQLASVALPGGNRAAATTQAENPYALIDQLARVLTWIEN
                     EYVDPVDRAKLLEGAIEGMVDKLDPHSAYLPPEDYAIFQSDTEGRFGGVGVEVDFGED
                     WVTVIAPIEGSPAERAGVRPGDRIVAIDHASVRGKSPAELVRRMRGEAGSKVLLTISR
                     KGSEKYLHFVLMREVINVSSVASKSLVGNVGYLRIKAFQVGTHAELLEHIAKLRKATR
                     TLPGIVLDLRNNPGGLVNEAAAVADEFLEAGVVFTTRRRGQVVDEVRAVPGGALRKEP
                     IVLLVNEYTASSAELLGAALQENRRARVVGTPTFGKGSVQTIVDLPGGAGMRLTTLRY
                     YSPLGHAIQAQGIQPDVRPPGATDDFGVIREKNIEGYLPAEQGASSTTEMPPIDPSAA
                     HPETPAVELPREIPVDPRKGNDVALRIAYEMLTASPTPAPKAPSVAAPKRTP"
     gene            12616..13011
                     /locus_tag="VFQ61_04430"
     CDS             12616..13011
                     /locus_tag="VFQ61_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953719.1"
                     /translation="MSSRPAGIARPGGTIGPAKIDAGPFDAGLLEPGPLEAVSCTQVS
                     SERVLSMLRIPRITMSKRLRARLDPSLNASLDASLQCDRILSASGCVLARRPIEVRPA
                     LVRRFLGPDSHAFGTNKARKIGGSCAQGT"
     gene            13245..13463
                     /locus_tag="VFQ61_04435"
     CDS             13245..13463
                     /locus_tag="VFQ61_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04563 family protein"
                     /protein_id="HET9953720.1"
                     /translation="MADNDSNKSAGSDKRKQSLYFPESMLQEIKDEAARLDRSLSWVV
                     QRAWKMARLEIKKIPSVNDVEDETEEAS"
     gene            14491..15030
                     /locus_tag="VFQ61_04440"
     CDS             14491..15030
                     /locus_tag="VFQ61_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9953721.1"
                     /translation="MQARGADGARFGATDKPVFVLCDGIVCDGYIYKYLWDDLAAYGG
                     VAHWNYRGHGRSASPVDVNRIHVIDHARDLDAVRTHLGDPEVILVGHSFGTQVVLEAY
                     RLRPERVRALILISGSYGRITYTFKNSDVLATVLPNLLDFAARHPRFARALWSRMPVS
                     LGMSLARMTGDIDLRSVRP"
     gene            15034..15333
                     /locus_tag="VFQ61_04445"
     CDS             15034..15333
                     /locus_tag="VFQ61_04445"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9953722.1"
                     /translation="MRPYFEHAAHVDFEMFLHMLREAGEHSAEDLLPRITVPTLVLTG
                     DKDSFTPAAVSEGMARAIPNSELHVVPSGTHILPLEQREEVARQIAAFLDRVPVG"
     gene            15345..16073
                     /locus_tag="VFQ61_04450"
     CDS             15345..16073
                     /locus_tag="VFQ61_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9953723.1"
                     /translation="MAQVQDPAVVAALLSLVAHDLRNPLSALQSNVGYLESTVGSQGR
                     ELREALVDVGSSCGSLKHLIDNLELLGLALLERTPEFERVPLSLWDMAGDVARRLEAI
                     AESYGVRVTLEGERQKGPWVLANRDMFSRCLSNLVLNAIQNAGSSSGVTLLLSQDAAW
                     GAVHVLDGGAVLADALELTAFTAEGQLLCKGDPRGRYGKGLGLFASRVAAELAGAEVS
                     SGHSADGRNLFILKARLVQNNLRS"
BASE COUNT         2954 a         4838 c         5419 g         3070 t
ORIGIN      
        1 gttttgggcg ggctgatctg gagtggggct ttgagttggt ttgggcggtt gccaggggat
       61 attcgatatg agggtgagca ggcgcgggta tacgtgcctt gggtttctat gttggtggtt
      121 tctcttgtgt tgagtttgat aggctacttg tttcggcgga tttgatagag ggcggcagta
      181 tcgtggggtg ttggcggtta ctgccagcac gacgattggt gttggtgccg gcaggggcct
      241 tcccgctgga tggaggttgg ctgccgaacc tctaagggtg aggcttggat ggggtttggg
      301 agttttgggt gcggggccct tcccaccggc gatcagagcc ccccgcagcg cctgttgtct
      361 cggtctgctc ggggccggac ctctcgagct agcggcggcg acgagtgttc tcgagagtgg
      421 ctgggttaac gcagtcgcgg gagttcgccg catgtggcgg cctgccgcat tcgagggccg
      481 cgagagcgcg aactagggcg cagccgcggt gggcgctcgt tcgagggttc cggggcggct
      541 gatcaggcgc gcgaggcctg cggcggcgaa catgtagatg aacggcatga ccggaaagtg
      601 gaagcgcggg ccgccgaaga agatggtgta caacacggtg aaatacgcga tcaccacaag
      661 agccgcgcgg atgcccgggg tgagcgcacg gcgtaggtgc aggacggcgt acgccgcgag
      721 cgcgaacgcc gcgagatagt tcatttgaga gagtaccctg agcggtagat agtagctctc
      781 accagttttc cgcagatctc ccagcctagc ccagccgaag ccgtccacgt cccaggtata
      841 aagagcgatg agctttccgg gtatccccgc gatcacttga agcggatgtt gctgcacgta
      901 gtgcaggaat acggatttgg ctcctgcgtt ccagctcaat tcgtcgctgt aacgctggac
      961 gacttcgtgg agttcacgat tccacatcaa gccctggttc ccccaagagc cgggcatgtt
     1021 gccgatcgcc agattcatcc aaccgttcgt cgagacagcc acccactcgc cgaaagcgag
     1081 gtagttgcga accgaccagg gcagcaccac cagacacaat ggcacgtaaa cgcaggccgc
     1141 tttctttagc cacggcccag cgctcagcca gagtcggatt gggcgcgccg accacgtcca
     1201 ttgctcgctc gcgcgcgagc tccgcccgag cgatgagatt tctgcaggtg tgtgcttgct
     1261 gaggggagga ggggaggtcg cttcgagcgc gcaatcctct cgtactgaac cagctcccga
     1321 ctcgcgggag agtgaagcgt agagctgacg ccacggagcc caaatcaaaa gcagggccgc
     1381 gggaaggaga gaggtttgag gcttggttag ggtggcgagc ccgaaaagca caccacttgc
     1441 caaggcccag cccaaagatc tgatgttacc gggtagcgca ccggtaggca ttcgcgtgtc
     1501 cggctgtgcc gcgtgggcca aggccaatac gcccaaaacc agcagcgccg tgtaatacgt
     1561 ctcgcaagcg gtgagcgcga catatgcaat ctgatttggc gcgaagccag ccacgagtaa
     1621 cgtcagccgc tcggtgagct ccgacttgaa ccaaagcctc gaaaggcgtg cagtacccca
     1681 aacggtgatg accgagagcg cgacgttcgc gagtttcgcg atcaccagtg gtgagccgag
     1741 cctatcgagc cagccctggc tcacataaaa cagcaccccc aagaaacctg aatagccgac
     1801 cggccagaaa gcggttttgc gcggcgttgg cagaggcgtg ccgctctccc acaacggaaa
     1861 acccgtgtgc tctacggtgt aaccctgacc cgatgcgatt cgcagtccct gcgagtagta
     1921 ccaagtgaag tcactagtcg gctgcacctc gatccaagcc acccaggcga tccggacgag
     1981 taccatgctg acaagacaca tcaacacgta gcgccgactg cggacggcgc gctcgattgc
     2041 gcgccaaact cgatcgcccg caagcgcgcc gatcgagctc caacgagcta ggcgcgatgc
     2101 ggaagattcg gtttcggtgt tcacgaggcg gtaatctccg cttgggacgg tctcggacct
     2161 aaagatggat aacgactagt acttcggttg gtcgctgaag aaactgctga tcttcaccca
     2221 ctcgcgatct gaccgtcgga acgctgcatg ccaatcacgc tttggcgttc cgaaacgagc
     2281 ctgtcgcacg cctgatcgta accgcgccgc gacccagcag tcacgatgcg gggcgagtat
     2341 ggcacaccga gtcctacccg cgccagagta ctattttgat gcggctcgga tcaggacgac
     2401 gaggcagatt agaaacgccg ttggtaaccg cgtcgataca cgcccccctg caagctcgct
     2461 actccgtcac gaggaatcga cacgtgtact tgatctcggt cgcgacgcgc tgcccttgat
     2521 gatctcgtgg agcgctccag cgcgagcctt tcaatgtgcg tcggcacgct tccgcgaagc
     2581 ccgtgaagga ctcacccaac acgtttacct ggcggactac gccatccgag tcgatgcgtg
     2641 ctctgacgct ggccgtgcct gcgatgccgc gacgtcgagc ttccgcagga tagttcgaac
     2701 gaagcgcggc tcctagctct ggagcggccg gcttggccga caaatcagcc atggccacga
     2761 gtttgggttc gctggtgtgc gcgggaagct ccgcggcttg gggggcgggg ttagggctgc
     2821 gccccgggtg gattgctgca agcggacccg tgcgcgattc tccattaccc agtggcgcgt
     2881 cgaagctgcc tgagtctccc gcgagcgtaa tccctgtcag atccacggtg cgcgcggcgt
     2941 tgggaggggg cggattctct gcaggcggcg gcgcactggg aactcgagtg gtgacgcttg
     3001 gccgagaacg ggccgggttg gggttggact ctggaactgg agtagaagct ggttggggag
     3061 gctcggggat tgccggcgcc gggggggagg cgagctcgaa gctaacggtg ctcttcaatt
     3121 cgcggcgcgc cgcgtgcgca ttcgagtccc cgagcgcgcc gtacagcagg gcgtgaaagg
     3181 cgatactcgc gccgagagcc catgcgagcc atggggcggt ctccaagttg gcttccacaa
     3241 tcaccggcct ttcagcgagg tttggcagcg aggtcatcgg ggctcacgtt gatggcgaac
     3301 tgggtgactt tctcttggcg aagaaggtcg agcagcgcga ccacgttgcg gtgcggaacg
     3361 gcgccgtcag ccgcgatcat cgctttgggc gtcgcgttgg ctgcgcgcag gcgttgaatg
     3421 cggccgcgta gttcgtcctc ggtggcgtgt tgcccatcga cgtacaattg cccgctcgga
     3481 tccaaactca ccgtcagcgg ggcctgcgcg ctctcgccgg tgctgccctt gggcagattc
     3541 agcgggatgc ttcgggtcgc gatatacgaa gccgtgacca tcagcaccac cagtaagacc
     3601 agcacgacat ccactagggg cgtgacgttg atactggtga tggcttcgtc ttgggatgaa
     3661 ttggcttggg ccatcggttc tcctcactcg gcggcttttg cgggtgattt gcggaacgcc
     3721 atgacctcgc tgatcagcgc ttcggctcga cctaggcggg cccggatgac gcgctggtag
     3781 aggttgaaga acgcgaccgc gggcagcgcc accatcaagc ccaccgccgt tgcgaccaag
     3841 gcttcaccga tctccgacat caaacccgac gagacctggc ctccggcgcc ctcgagctcc
     3901 cggaatgcgc gcacgatccc gatcacggtg ccgagtagcc cgatgaaggg cgcgttgttg
     3961 ccgagcgtcc cgaggagggt gaggttccgt tccatggcga gacgggccag ctccgtctcg
     4021 ccgcgcatgt gttcgctggc gctctcgact gtctccgaat cgaggccacg cacggcaatg
     4081 cgcgcctcga agctaggaga ttcttggagc ttgcggcggg cttcgtcgag ctcgccgcgg
     4141 ccgagtagcc tccgcaagtc accacgcagc cggtggacat cgtctttcga cgtagcgaat
     4201 aggaccgcgc gctcgatgac gacggcgagg gcggcgacgg atagtgcgac gagcacccag
     4261 agcacccaag tggctccgtg tgcggtgaga gagctcaaac gattgacgag gttcatggta
     4321 atactcagcg ttcttggaac tttggttgag gagacgagtc gacctgacga gtcgacctga
     4381 cgagtcgacc tgatataggt gcaatcagtc gacgcgatcc cttcgagcaa tggatcggtc
     4441 gtaagaggaa aacgtccgag ggcgagtcac ggtatcaagc acacgctgcg ctcgatgtac
     4501 gcggtgcccc cgcgctcgtc gcgcaacaca atccagaagt gcgcgcgcgt acccggcgct
     4561 tccaccgtcg caggagccga ccatggcacg cgcaccccgg tattctcctc gctccagctc
     4621 aaccgcgaga atggccgatc caacgagccc gcgctagcaa agtgagagac gaggatactc
     4681 tcgcgcgtag gatcttcggc gagcactggc gagacgggct cgcgcgatcc cgcgtcgatg
     4741 cgtatttcga gctcgtgaat cgttgcgtcg gcggcgagct gagggaccag ggcgctgccg
     4801 cagtcgaaag gctccggggc tgcgagccag ggtgctccgt cgagcagaat ggctggctct
     4861 tcgaacgcag ggttctggtt cgtgaactcc ggagtctcca agttcaaatc gaatacagcg
     4921 cgcagccctg tctgcccgcc ggtgcaggcg ccgctctcgg tcgcctcgct acccgcacag
     4981 aaggtgccct cgagatggat acgaggatga gccacggcgt cccacgtctc tggcagctcg
     5041 aacgtgaact cgagctcgga gccttccacc tcgcttcgat tggccagcgc gaaaggcgcc
     5101 tccaaacagt cgccggtgcc cgagttccag tcggcaccca tgcaggccga gaagcccgcg
     5161 ccgatgcgtg gctgcggatc ggggctggct acgagccagc gcacgctgac ggcctcgcca
     5221 ggacgcggag tggcccgcga tggctcttgc gacgcctcgc tgcgcgcagc cagaacacgg
     5281 ctgcgtgtaa tgtcctgggc gcgctcgatc gggtcgtcgc agccgaacga caggcaagag
     5341 attccgagca cgtagccgaa accaagtaga ccgattgaac ttcgtttcac agttcacccc
     5401 gcagacccag attcggaaaa atgggcattc ccgtcacgga ctcgctcttc gaatagtcga
     5461 agttgtagcg agtcccttca ggatgttgag cgttgtacac gttgagcacg tcgagatagg
     5521 cggcgagctt gacggggccc gcatgccaca gcttttcgag tcgcacgtcg agttggtgaa
     5581 acgcgggcgt gcggtcacta ttggtgtcgc cgtagatggg tagatagctg ccacttctgg
     5641 cgtcgaatac gctgcccttc accggcgtag tggggttgcc ggtgacatat cgaaagcggg
     5701 ccccgagctc ccatccttta ccgagtggct ggctcgcaac gagcgacagg atatgggtct
     5761 ggtcccgatc gaacaggcgc atgggcgcgc ccgggcgatc gcggcgctcg ctgcgggaga
     5821 gcgtataggc cagatacaag aaggcgttgc tcggagtgcg ggcctgcgcg gagagctccg
     5881 cgccgtagat gtttccggta cccgtgtttt gaaagtgggg cagggcatgt cccggtgtgg
     5941 cgctgacccc gttttggatc cgcttgtaaa acgcctccgc cccgagctgt gcgcgcgcgc
     6001 cgaggcgctg ttcgaagccg gcgctggctt ggatggtgcg atacggttcg agcttcggat
     6061 tgccgacctc ccgtagccgt tgccagaact cgagctcctg cgagaagcgg ccgacgccgg
     6121 ccttgatcgc ggttttcggg gttacctccc agcgcagcgc gaagcgcgga tcgacttgta
     6181 aagacttgga ctcaccgaga tagtcgacgc gcactcccgg aatcaggaac aactccggca
     6241 ccggtcgata agcgagctcg acataacccg ccggcttgat caacgtcgtt gcggggagct
     6301 tggccgtgat ggtgcgctga ccattcagcg cgtcttgacc gctcgggtcg ccttcgaatt
     6361 gaccaggata ggggccgcga tactcgccgg tgacgatcgt ggaagcgagg tctcctccga
     6421 gcgtgaggcg caaacgtggg tcgagctcca agcgagtctc tgcgcgcgca tatagctctg
     6481 ggccgcgcag cacttgtcgc aggtcaccga tgtattgctc aatcccgagt tgacccacgg
     6541 tcaaactgaa gtggctctga actgcctgac tgggctgcgc ctcgagctcg agccccaggc
     6601 ggtgaaactc gatctttcca cgaatcgagc cggcgagagc gggatcgaaa tcggtgggat
     6661 tgctgaagag aagctcgagg gtatctcggc ttccgtaccc gagtacacgc agcttcgtgt
     6721 gcgagtcgaa gcggtgcacg ccgaagagct ggtagtcgaa gtacaccgga gccgcgacta
     6781 ccgagtatgc atctttcggc acgaagtttt cgaaaacgaa atcgatgttg ctgcggcgga
     6841 cggcggccgc gacgtgcgtt cgctccccaa gtggtgcttc cacgaatgcg gaagaatcga
     6901 tcaggttcaa gtccaatgcc gcccgcagct tcgaagcatc gggatcgcgc gggcgtactt
     6961 cgaccacacc tccggcgatg cgtccgtacc gcaccgagaa gtttccagga tagaagtcga
     7021 tgcgggagac catgcgcgag ctgaggaagc tcgtgagccc gccgaagtgg tacaggaacg
     7081 ggacgcgcgt gccgttcagg agagtggcac tttcgttgaa gttcgcgccg cgaatcaagg
     7141 gcgcgcccga gtcgagggag gtgcgcgcca cgcctggcat cagttcgatc gcacgcagcg
     7201 cgtcgccgcg cgtgccgggc gcgcgctgaa tcgtggtttc gtcgacggtg cgacgcacga
     7261 cttcgctggg gggcgcttcc acgctggcgg tggcttcgaa ttcgggcaac tcgtctttcg
     7321 cggttgcgtt cgcgttcgtc atcggagccg cgttgctggg cgcggcggca gagggcgagt
     7381 ttgggctgga aaccgcggta gattgacctg ggccgtggct cgatcgctct ccggccacgg
     7441 ggcgagtccc cgcctcggcc gcggcggctt cgggcgctac ggctgccgga gcgaactcga
     7501 gcaagtaccg gattttgacg cgcacgggtg tgcctgcgtc gcgcgccggc gtgaatttga
     7561 gctgacgcgc ggcctctagc gcaaggggct cgagggtggc gtcgctcgat tgcacggctt
     7621 ctactccgcc gacggagcca tcctcgagga tgattagctc gagcagtacg cgcggcgggg
     7681 aggagttgga gctcgagtga ggagcaaccg gtcgcacgcc ttcggagaga cgcggcggtt
     7741 cgattgcagc cgtcgaatcg ggcgcttggg ctgcagaatg cagcgatgcg gaacacgctg
     7801 cgagcccgag cagcgcggca aaccatctgg attttcgaag cacgcggctt ggtgcccgct
     7861 gctttcgaaa gtgttcaaag cccggcgcac gattcgccga ccgtcaacgg agggggccgg
     7921 cgtggtgctt cgggaacggg agggcggagg gggctgcgct ggcgagggtc ctggcctggc
     7981 cgcgcgctct ttacacagcg gccgaaatac caatgaggct agcgcagctt ccgacgtaga
     8041 taattgagca cgtcgatacg tgtaatcaaa cccaagaagg tcgacccctc acaaatgatg
     8101 gggaccaatc cgcgatcgaa caacggcaga acttcggcga gcgggcgatc gacttggacg
     8161 gtgacgagct tggtgctcat cgtgctccga acggagcgac ggaacgcgct ctcgtcgtcg
     8221 tgcacggcca gcaacagatc ggattcatcg atgatgccga cgatgcgctc ggcttccaag
     8281 acgggtagct gcgagacgtc gtacagcttc attctggagt aggcgacgag cagcgtgtcg
     8341 tcgggggtca aggagatcac ctgctcgtgc ttgcgagcga tgagatctcg gaggtcgccc
     8401 tgatcggggc cgcgcaagaa gccctgatct cgcatccaga agtcattgta catcttggac
     8461 aggtatttgt tgccgctgtc gcagacgaag gtgaccactc gtttcggttc ggtttgcgct
     8521 tggcagtagc gcagtgcggc cgccaacagc aaacccgtcg aggagccgcc caggatgcct
     8581 tcgcggcgca gcaacgcgcg cgcggtgagg aagctctctt cgtccgagac actgtaggcc
     8641 tcgcgcacgc gcgatagatc acagatcgat ggcacgaagt cctcgcctac accctctact
     8701 agccatgagc ccgcgttccc gatcttgccg gtcttgacgt agtcgctcaa tacggagccc
     8761 acgggatccg cgagcacgat ctcggtggcg ggcgaggctt tggcgaagaa gcgactgagc
     8821 ccagtgatgg tgccgcccga gccgaccccg cagatcacgg cgtcgacccg accctgcatt
     8881 tggcgctgga tctcggggcc ggtggtgcgc tcgtgcgcga gggggttcgc ggggttgttg
     8941 aactgattca cgtagtaggc ggagtgctcg cgggccaggc gttccgcgag gtcttggtaa
     9001 tactcggggt ggcctttgcc gacgtccgag cgggtcatcc gcacctcggc gcccatggcg
     9061 cgcaggtgaa agatcttctc ttggctcatc ttgtccggaa tgacgagcaa caagcggtag
     9121 cccttgcgag cggcgacgag cgcgagcgag aggccggtgt tgcccgcggt ggcctcgatc
     9181 agcatcgcgc cttcgccgag cttgccttca cgctcggcgg cctcgatcat ggacagcccg
     9241 atccgatctt tgatcgagcc gcccgggttc tggttctcga gtttgacgta gagttcgcag
     9301 ggcccggtgt ctagggaggt gagccggacc agcggggtgt tgccgattag cgagaggacg
     9361 tcggtgttgc cggtttcacg gggattcgtg ggaacatttt gcatcgcaac tccttcgcaa
     9421 gaggccaggt gatcaggttt ggatatcagc tcgcgccgag cgcctgctcg aggtctgccc
     9481 acagatcctc gacgtgctcg attccgacgg agaggcggac gagcccatcc gcgatgccaa
     9541 tcttttcccg cgtctcgcgg gggatggatg cgtgcgtcat caatgcgggg tgttcggcga
     9601 gcgactcgac accaccgagg ctctcggcga gggcgaacca gcgcagcgat ttgaggaagc
     9661 ttcgcgcggc gggctcgccc gctttcaaga ccaagctcac gatcgccccc ggcccgcgca
     9721 tttgccggca tgcgagctcg tgttgcgggt ggctagccag cccggggtag tgaacgcgct
     9781 cgacgctcgc gtgctctgcc agtcgctccg ctagtttctg cgcgctggcg accgcgtatt
     9841 gcatgcgaac tgccaaagtc ttgattccgc gcaataggag atagcagtcg aaggggctgg
     9901 gcacgccgcc cagcgcgttc tggagaaaac gcaggcgctc cgccatagcg tcatcgttcg
     9961 tgacgagcgc gccgccgacc acatcggagt gcccgttcaa gtatttggtg gtggagtgca
    10021 ggacgatgtc cgcccccaac tcgagtggcg tctgcaacgc gggggacgcg aaggtgttgt
    10081 cgaccagcag acgcacccca cgctcacggc agagtcgagc gatagcggcg atgtcactca
    10141 gcttgagcag tggattcgtg ggtgattcga gccaaaccag cttggtgcga tcggtgaacg
    10201 cggccgcgac ccgagtgggg tcggttgcat ccacgaagct ggcttgaata ccgaagccgg
    10261 acatcacccg ctgaaacaaa cgataggtgc caccgtacac gtcgtcgctc agcactacgt
    10321 gatcgcccgg tgaaagcgaa tgcaagagcg tggttgccgc ggcgcaacca ctggcgaagg
    10381 cgaagccgtg ctttcccttc tcgagcgcag ccaagcaggc ctcgagcgtg gcgcgcgttg
    10441 ggttgccgct gcgcgaatag tcgaagcgga gcggctgtcc cggctccggt tgggcaaacg
    10501 tcgtagacag caccagcgtg ggcattaccg cgccatgcac cggatccggg ctctgcccgg
    10561 cgtgaatcag tagggtctcg agggcagggg aacgcatagg aggtacctct gactatgcca
    10621 gggcccagcc cggacggtaa ccagcgaagc tggtaaccca tctggagtgg agagcccctt
    10681 gtggccggcg atccgccttc gtgcggtttg ggcgcaatca ggttcgagcg attcctgcga
    10741 accggcgcgt gttctcgtca ctggtcaggg cgtgcgcttt ggcgcagcca cggacggggc
    10801 cttcggtgcc ggtgtagggg aggcggtcaa catctcgtac gctatgcgca aagcaacgtc
    10861 gtttcccttg cgcggatcga ccggaatttc gcgtggcagt tcgaccgcgg gcgtttccgg
    10921 gtgcgcggcg ctcggatcga tgggcggcat ttcggttgtc gagctcgcgc cctgctccgc
    10981 gggcaagtag ccctcgatgt tcttttcgcg gatgactccg aagtcatcgg tcgcgccggg
    11041 ggggcgcacg tccggctgaa tgccttgggc ctggatcgca tgaccgagcg ggctgtagta
    11101 gcgcagagtg gtcaaccgca tcccagcgcc accgggcaaa tctacgatcg tctgaacaga
    11161 gccttttccg aaggtcggcg tgccgaccac gcgtgcacgc cggttttctt gcagcgcggc
    11221 gccgagcagc tcggcggagc tggccgtgta ctcgttgacg agtaagacga tgggctcttt
    11281 gcgaagcgca ccgcctggca cggctcgcac ttcgtctacg acttgcccgc gacgccgcgt
    11341 ggtgaacacg acccccgcct cgaggaactc gtccgcgacc gccgcggcct cgttgacgag
    11401 acctccggga ttgttgcgca ggtctagtac gatgcccggc agggttcggg tggcctttcg
    11461 gagtttggcg atgtgctcca acaattcggc atgagtgccg acttggaacg ctttgatgcg
    11521 tagatatccg acattgccaa cgagcgactt actcgccacg gaggagacat tgatgacctc
    11581 gcgcatcaac acgaagtgaa gatacttttc gctacctttg cggcttatgg tcagcaacac
    11641 cttgctgccc gcctctcctc tcattcggcg caccagttcg gctgggcttt tgccacgaac
    11701 acttgcgtga tcgatggcga ctatacggtc gcccggacgt accccggcgc gctcggcagg
    11761 tgagccttca attggcgcaa ttacggtcac ccagtcctcg ccgaagtcga cttcgacacc
    11821 gactccgccg aagcgtcctt cggtgtcact ctggaaaatt gcgtaatctt ccggaggcaa
    11881 gtacgccgag tgcgggtcca acttgtcgac catgccttcg atggcaccct cgaggagctt
    11941 ggcgcgatcg acagggtcga catattcgtt ttcgatccag gtgagcactc gcgcgagctg
    12001 gtcgatcaac gcgtaggggt tttcggcctg cgttgtcgcg gccgcgcgat ttccgccagg
    12061 tagcgcgacc gaggcgagct gcgacgcaca aaatcccaat accgcagcaa tgacgagtgc
    12121 tcgggagcgc cacctccgct cgaagcgcgg tgcacgcagg acgtcaccgc gcttcgcgag
    12181 cgctgtactt cgcctcgcac tttcggattt cggcggcagc tcgcgtgccc gatgcgctaa
    12241 cgcgagtgcc aggctgcggc gctccctcca agaggagctc gaacacgtgg ccgcacgagg
    12301 cccgagcgat gcgcatgcgg gcttgggtac gacagagcca tgctgcacgg ctgctacggc
    12361 tgcacttcga gcatttttaa gcgagaacac gccgcaaatt gagcctgaac ctgcgacgca
    12421 gttttgccgc gcgttcgaat ggctcgagca cccgccagaa cgcccgctgc aagtcagcac
    12481 actgaaagcg agcttcccgt cgctcctggc caggtcgagc ccggaaaggc ctgagcgctg
    12541 catagagctg tgcgctgacc tcatcgacga gcgattcagc gcgttcttgc cggggggcca
    12601 gcgggaattc tgctcgtgag tagtcggcct gccgggatcg cacgtcctgg cgggaccatc
    12661 gggcccgcca agatcgatgc cggcccattc gacgcgggtc tgctcgagcc cggcccgctc
    12721 gaggccgttt cctgcaccca ggtgagctcg gagcgtgttc tctcgatgct ccgaattccg
    12781 cgcatcacca tgtcgaaaag gctccgcgcg cgcctcgatc caagcctgaa tgccagcctc
    12841 gatgccagcc tccagtgcga tagaatcctg tctgcatcgg ggtgcgttct cgctcgtcga
    12901 cccatcgaag ttcgacctgc tctcgtgcgg cgcttccttg gaccagattc gcacgcattc
    12961 ggaacgaaca aagctcgcaa gattggaggc tcgtgtgcgc agggcactta agaaactcgt
    13021 ggagcgaccg cgaatgagag cagaacgccg agcaggagca ctgcgagcag gagaacggtg
    13081 aagcgagttg cgtggcgcag gatcgatcga catgaggtgc cccacaatac cccattgctt
    13141 ccccgggtct ccgggtgcta ctaaggtact aaccgaaatg gagggtcggt cctgatgatc
    13201 ctgctgacca tggaccgact gccttctttt gatcagaggt aagcgtggcc gataacgatt
    13261 ccaacaagtc cgctggaagc gataagcgca agcagagtct gtatttcccg gagtcaatgc
    13321 tccaagagat caaggacgag gcggcgcgcc tggaccgatc gctctcgtgg gtcgtgcagc
    13381 gagcgtggaa gatggctcgc ctcgagatca aaaagatccc gagcgtgaac gacgtcgaag
    13441 acgagaccga agaagcgagc tgacgggcac acccccggct cgatctaaca tcaacgtgta
    13501 gctcggctca gctcaacggc tgggccctgc gcgtggcggc gctcatgacc tgcccaaaga
    13561 acttggcgaa gagtcaagcc ttgtggcgag gtgaaccgga gggaattcga agagggtgaa
    13621 ccctaggaga attcgctgcg ggttccgtgg gccttcggca ggaacactcg gctacgtgtc
    13681 gccggacgcg cagcgggtgt ttctcgattg acggcttccg gcgcgtgacc cgctccgcaa
    13741 tgacgagttg cacagcaaat ctgactcggc acggcgttga gccgatgcca gccaggtttg
    13801 gtgtgtcgcg cgtgctttga atggcgtgca cgttcgatgt agcccgcgca catgttcgta
    13861 cacgtgatcg aactctttcc gacgaagcat cgatgcgggc cgaaaagagg gcgttcgaag
    13921 gattgagaga gttcgcccat ctggctcgga gagcccgtta gacgaactgc tcgatgacca
    13981 atccgaaggg ctggttcgaa aacacgttcg gacgacctga gcagtctacg gattgtttcg
    14041 gtctgttgac gccggctttg gatcgttacg gtgcgtgcca gacgcgacgc tgagggctac
    14101 cctcggtccg tggattcttt tgcttacggt cctgacggga cccgcctgta tgtccgttcc
    14161 aggcctcggc tgctgcccgg ggccggttcg gtacctccga actcgacggt ttcgggggcc
    14221 ttatttccgg cgagctccag actaaccagc gttcctccga gcggtgcggg ggttggcgat
    14281 tcgccaattg gaggctcggc ggcacacaac tcggcagtgg gcggcttgcc gaggagcgtc
    14341 gcgccctcgg agccagctcc gccgtcaggg aggccgagct ttcggtcggt tcgaccgggc
    14401 gaaagtgtca gtgcgccggg ctctgcgcgg tctccgtcga gcgggatctc ctctcctacc
    14461 aggccttctg ccggaccgcc tgatgcggga ttgcaggctc gtggtgcgga cggcgcgaga
    14521 tttggcgcca cggataagcc ggtcttcgtg ctgtgcgacg gcatagtgtg tgacggatat
    14581 atctataagt acttatggga cgacttggcc gcgtacggcg gtgtcgccca ctggaactat
    14641 cggggacacg ggcgcagtgc ttcgcctgtc gatgtgaatc ggattcacgt catcgatcac
    14701 gcgcgagact tggacgcggt gcgcacgcat ttgggagatc cagaggtgat tttggtgggg
    14761 cactcgttcg gtacgcaggt cgtgctggag gcctatcgtt tgcgcccgga gcgcgtgcgg
    14821 gcgttgatct tgatctcggg ttcgtacggt cgaatcacct acaccttcaa gaacagtgat
    14881 gtgctggcga ccgtgctgcc caacttgctc gactttgcgg cgcgacatcc acggttcgca
    14941 cgcgcgctct ggtccaggat gccggtgagc cttggcatgt ccttggcgcg catgaccggc
    15001 gacatcgatt tgcgctcggt acggccctag gacatgcgac cgtacttcga gcacgccgcg
    15061 cacgtcgact tcgagatgtt tttgcacatg ctgcgtgagg cgggtgaaca cagcgccgag
    15121 gatctgctgc cgcgcattac ggtgccaact ctggtgctga cgggcgacaa agattccttc
    15181 actccagcag ccgtttcgga gggcatggct cgcgcgattc cgaattcaga gctacacgta
    15241 gtgccctccg gcacacacat cctgccactg gagcagcgcg aagaagtcgc gcgccaaatc
    15301 gcggcttttc ttgatcgggt gcccgtggga tagtcgactg cgacgtggca caggttcaag
    15361 atcccgcagt cgtggcggcg ctgctctcac tcgtcgctca cgatctcaga aacccgttgt
    15421 cggcgctgca gagcaacgtc ggttatctgg agtccacggt cggctctcag gggcgcgagt
    15481 tgagagaggc gctggtggac gtcggatcct cgtgcggctc gctgaagcac ctgatcgata
    15541 acttggaatt actcgggctc gccctgttgg agcggacacc ggagttcgag cgagttcctt
    15601 tgtcactctg ggatatggcc ggagacgtgg cgaggcgcct ggaggcgatc gcagagagtt
    15661 acggggttcg cgttaccctc gagggtgagc gtcaaaaagg cccttgggtg ctcgcgaatc
    15721 gggacatgtt ttcgcggtgc ctgtccaact tggtgctgaa cgccattcag aacgcgggaa
    15781 gctcgagcgg cgtgacgctc cttttgtcgc aggatgcggc ctggggcgcg gtgcacgtgc
    15841 tggacggggg cgctgtgctc gccgacgcgc tcgagctgac ggctttcacc gccgagggcc
    15901 agctgctctg caagggagat ccgcgcgggc gctatggtaa gggcctgggt ttgttcgcca
    15961 gtcgggtggc ggcggagcta gccggggccg aggtgtcgag cggacactcc gccgatggac
    16021 gcaatctctt catcttgaaa gcgcgactcg tgcagaacaa cctgcgctcc tgagcccgtg
    16081 tgacgtaagt acgggatgct cccaacgcgc tgacgttcgg gggctggtca gggctgcagg
    16141 atcactactc gttctggttt gcggaacaag attttaccgg tctcacccgg acagccccag
    16201 caggtgctga agtgcaggcg aggtcgcagc ccgttgtcgt atgcaaatgc gacggggcca
    16261 ggctcgttct tcaggatgaa g
//
LOCUS       DASTKU010000123         2604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1807235, whole genome shotgun sequence.
ACCESSION   DASTKU010000123 DASTKU010000000
VERSION     DASTKU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807235"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VFQ61_04455"
     CDS             complement(<1..768)
                     /locus_tag="VFQ61_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953724.1"
                     /translation="MMLLSAVSASLASWLSGPSDFFWGNPIFEKDAWEAFTKGFRVGI
                     ALIGALAIVYEIRARRMGERIPERTRRRLGWLFTILAFGVYFDFGNPNTRYSEYYHRH
                     ELYHYYLGSKYFKEVGYTRLYECSLIAEVENGRLDQVKNREVRDLRVNLIKPVSDTYI
                     LKDPDQCKKYFTTERWDTFKREIDWFYHSAAGSYWEGMQKDHGYNPPPVWSMTGKFFG
                     SFHEASDGFFKILASLDVLLHLGIVLLFRWAFGWRVMA"
     gene            complement(1465..2007)
                     /locus_tag="VFQ61_04460"
     CDS             complement(1465..2007)
                     /locus_tag="VFQ61_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953725.1"
                     /translation="MVARALAFCLSVVAASCAPYDSKHDAKIPGELLGTYDVSGQLKS
                     DSCGAELLGAPNPWRFDLKLSRFERDLYWLNGREAIVGDIDADGTTFHFRTRIDVPLT
                     PAAKGAAGCIVSRYDQADGKLVAGDGDSVTAVTANLSFRYESKSGSECLEIIGIPGGV
                     QQLPCTLSYGLGGSRLDQGP"
     gene            complement(2061..2225)
                     /locus_tag="VFQ61_04465"
     CDS             complement(2061..2225)
                     /locus_tag="VFQ61_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953726.1"
                     /translation="MANRAIWNVRMRHEWLATPLKGKRLSKRKRARIGIEQARERKAP
                     APAGSTPAAS"
BASE COUNT          494 a          836 c          829 g          445 t
ORIGIN      
        1 ggccatcacc cgccagccaa aggcccagcg gaacaagagc acgatgccga gatgcaggag
       61 cacatcgagg ctcgcgagga tcttgaagaa gccgtcactc gcctcgtgga agctaccgaa
      121 gaacttgccg gtcatgctcc agaccggcgg cgggttgtag ccgtggtcct tctgcatacc
      181 ctcccagtag ctaccggcgg cggagtgata aaaccagtcg atctctcgct tgaacgtgtc
      241 ccagcgttcg gtcgtgaagt acttcttgca ctggtccgga tccttcagaa tgtaggtgtc
      301 cgaaactggc ttgatgaggt tcacgcgtag atcgcgcacc tcgcggttct tcacctgatc
      361 gagccggccg ttctcgacct cggcgatcag cgagcactcg taaaggcgcg tatagccgac
      421 ctctttgaag tacttcgagc cgaggtagta atggtagagc tcgtggcggt ggtagtactc
      481 cgagtaccgc gtattcgggt ttccgaaatc gaagtagacg ccgaacgcga gaatcgtaaa
      541 tagccaaccg aggcggcgcc gagtgcgctc ggggatgcgt tcgcccatgc gcctcgcgcg
      601 gatctcgtag acgatcgcga gcgcgccgat cagcgcgatg cccacccgga agcccttggt
      661 gaaggcttcc caagcgtcct tctcgaagat cgggttgccc cagaagaagt cgctcggtcc
      721 ggagagccag ctggcaagcg aggcggagac agccgaaagc agcatcataa ggggccaaaa
      781 cctcccgccg cggacgcggc gcacggcccg cgttcgtaga ccaatgccgc tcttacggca
      841 accccgccgc aataatgccc ggtcacatcg gacgcgcgat tgacgatcac gccccgattc
      901 aagcccccga aattaaaaac gggccgggtg gctctttgag cacccggccc gccgtagcga
      961 gcccagaaac cccgaaggtc ccaggtttgg tcggtgtcct tagagcgcga tcaacgcgcg
     1021 actcaggact caccgccccg ccgatcgtga acgaagcacc cggcctcgcc cagaccagca
     1081 cgcctgatcg ctgttcaggc ggctcgttgg ttcgctcccg cgctaggcgg gcaaaccggt
     1141 cgagctaggt tgggcacacc tccggtccag ggatacaact actcttcatt gttgctctac
     1201 ctcctccggc aatagccgga cgcccgccca ggcgctccgg agagcaaatc tgctcgatcg
     1261 gcgagctccg ggttctgggg cgcttccgaa agggaggccg ctcacaaggc gacctccagc
     1321 cctgtgtcgc tacgggggtg ctcccccgcc ggaaaattcc gggagacgtg ctccgcgctg
     1381 acaccttgag catgaacata acacgctcgc ggcgatccac cacccgcccg ggtggagctc
     1441 gttcatttgg ctagggcaga gcggctaggg gccttggtcc agtcggctcc cgccaaggcc
     1501 gtagctcagg gtgcacggaa gctgttgtac gcctccagga atgccaataa tttcgaggca
     1561 ttcagagcca gacttggact cgtacctgaa cgataggttg gcggtcacgg cggtcacgct
     1621 atcgccatcg ccggccacca gcttgccgtc ggcttgatcg tatcgggaca cgatgcagcc
     1681 cgcggcgcct ttcgccgctg gggtcagcgg cacgtcgatg cgtgtgcgga agtggaaggt
     1741 cgtcccatcg gcgtcgatgt ctcccacgat cgcctctcgg ccgttgagcc aatagagatc
     1801 tcgctcgaac cgcgacagct tgaggtcgaa tcgccagggg ttcggcgctc cgagcagctc
     1861 agccccgcag ctgtccgact tgagctgccc gctgacgtcg tacgttccga ggagctcccc
     1921 cgggatcttg gcgtcgtgct tggagtcgta gggagcgcac gaggcggcta cgacggagag
     1981 acaaaacgca agtgcccgcg cgaccatgct cgccccatag cgagccgcgg ccgggcgggc
     2041 caaattcgag cagatcgagc tcagcttgcg gcgggcgtgg aaccagcggg agccggcgcc
     2101 ttgcgctcgc gagcttgttc gattccgatg cgggcgcgct tgcgcttgct gaggcgcttg
     2161 cccttgagag gagtcgcgag ccactcgtgc cgcatgcgca cgttccaaat tgcacggttt
     2221 gccattcgag gggccagcct ttagcgaagt tttccaggca acgcaagccc gagggctctt
     2281 tcctcacttc aatcgacgaa agcgaccccg ccaagtccca ccagccgaaa tcgcgcgagg
     2341 tggcggcgac cgcctcaatg ggcggcttgc gcggcggggg cggcgctcca tcattcgcga
     2401 gcgccgctca cacccagggc agccgaggac gaccgaaatg cgcgtgacga tggtgaaaaa
     2461 gcagttccga gatggctcca cctgcaaaaa gtgcgagcag gcggaggatc tcttgcggcg
     2521 tcgcggcctt tgggcgcgca tcgacgaagt cgtgtgggcg cacgaggcgg acccgagctc
     2581 gccggggatg cgactcgcgg agca
//
LOCUS       DASTKU010000124         8167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_23892,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000124 DASTKU010000000
VERSION     DASTKU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23892"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFQ61_04470"
     CDS             <1..235
                     /locus_tag="VFQ61_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953727.1"
                     /translation="AYTQTSAGWVVQTTAMHGVWQPLSGGCHFIYDSGMGALDNIPSG
                     NKHRLVAAAYQLYVTGRVYRKVRTRLYYDVGPC"
     gene            complement(554..1222)
                     /locus_tag="VFQ61_04475"
     CDS             complement(554..1222)
                     /locus_tag="VFQ61_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET9953728.1"
                     /translation="MKSVVAGLVLICAAGCSSDSKSDSSPKELEVFPPQLFSGFDGMH
                     TYKAPAIAVNKSGAVTWTIADASIASLAPADDGEHVTITSLKAGETTITATSGAQKST
                     VPLKVYSYTTQQWDAGHTRYTTAADANNPECIACHSKNAGPDHTPTEVDADPDTEIQN
                     TFLTGVDPEGRPIKDNSEFAYLLQGKNHTWKVTDAEKSGLVAYMRSLEPSGWPEYDAP
                     TAGK"
     gene            complement(1548..2591)
                     /locus_tag="VFQ61_04480"
     CDS             complement(1548..2591)
                     /locus_tag="VFQ61_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953729.1"
                     /translation="MTRTNRTALMLAWTTRLSLVLAMCSALSACADGDPPYDDVTGKL
                     DADLLSNRGFYADLASGTPPENALEYEPDYALWSDGAVKRRWLILPPGAQIDTSDMDH
                     WQFPVGTVAVKEFSLEGKRLETRVIERVGDSGVQKEDYRLGTFVWRDDQSDAVLTSDG
                     VPNVNGTTHDVPAQKFCMHCHKGEPGTVLGFSAMQTSKSGWLSRLVELGAVTNAPDHS
                     FAIPGDATQSKALGILHANCGHCHTDKGMAPKMRLRLRTEEAELPFEETELYQTTVGQ
                     RISRDWLAPPPEYTIRIAPGHPESSAVLYRMEQRGDDDELVPEQMPPIATNKVDEEAV
                     AVIRDWIAALPEL"
     gene            complement(3511..4065)
                     /locus_tag="VFQ61_04485"
     CDS             complement(3511..4065)
                     /locus_tag="VFQ61_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953730.1"
                     /translation="MSISLGRTFVSGLVSAGLIAALSSQVQADSRHGRCKEITGPFSS
                     VTVPVPPCTSPVGLCTHGILGGPMDNASYDFTVYSTEPSPDDPNTIIASGKSVITTSR
                     GVMMTNDVSVLHFTGPAPTDPVDFVTTATIETGTQRYKATSGQFVAMGTLSFATGQAS
                     GSYAATLCEGRRPGHGHGHDHDCD"
     gene            complement(4178..4678)
                     /locus_tag="VFQ61_04490"
     CDS             complement(4178..4678)
                     /locus_tag="VFQ61_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953731.1"
                     /translation="MLPIIESWRTVATSPDYALATWQQVFAVIWRRETTLEGAGHLRS
                     ACTAFAQHHPKGIGLLTIVESGAPLPVSAVRKEIAAFLGDASSFIKCSAVIFEGSGFR
                     AASVRSVVTGLTLLARQAYPHKVCDLDEAARMFSEILPRATGRALSSEAMRSSLAELR
                     STLDKS"
     gene            complement(5166..7118)
                     /locus_tag="VFQ61_04495"
     CDS             complement(5166..7118)
                     /locus_tag="VFQ61_04495"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9953732.1"
                     /translation="MTSAEPESGSRLALDGFLRSHLLVGDSSVFDPCLPGLSRVTGEQ
                     ARAPTGSQLAAVRIQFEALGLNTARHSLAWVNDRLVEGTIFRIDEREEEVELIDDAAR
                     IRGIVSALIASEARGHAMLHGSARGERRRTVELRLTREGNFLWSVPPDASLQGSAIEV
                     QVQGFSSVYLFDVPVRNALLPPTTMVRVRRRRFRRVAAPRRLRLTAPHPLWREIVIDA
                     RATDVSEQGVCIETDAVRDMLYPGLRLKSVELRWKGGQPIKAEAVVAHVTQQSSSALH
                     RVGLRLLLRGAEASRWHLELEELLHPRTTRGEPDASTFWKVYVDSGYFHLSGKSEREF
                     VDERAAFEQSQEHFRGAPDVAAFFASSSKRRTESVAHQTSLWHGSWIYYQFCRLPEMR
                     PLSAADDGLLLDLYAHAYEHVQSRPDARWLVTYVQEVARFSRLIFYDVAERYRAQGRA
                     SIWRFRALEVDCAQGDSSELDYEVSQARALEISALKNRIHQLRSQIYIEATGLSASDL
                     GIGASIEAWEAAGLMRARVVLIARTKAGSPVAGAVLDAVNAGVHLFGLLDMARLFPLV
                     DGGEDAYDSLLAAARRWFGGLGKRKFTYFADESEDPEASKRSVTDLGRAFMTILPVEL
                     LPELLEHVFCVTCVNQPSSLSQSSTP"
     gene            complement(7486..>8167)
                     /locus_tag="VFQ61_04500"
     CDS             complement(7486..>8167)
                     /locus_tag="VFQ61_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953733.1"
                     /translation="DALATEARAAGANLDLLVDADELDRIGEIIARGERLRLLNGRMH
                     REMMDEVRWNARDVASTRDGLDVATLELTPTDLAGMRLVSTWTLMDLVGKLGLGRGLE
                     RPSRKAVASASAVGLLTAPGRGKSDWFHGGRALERVWLRSTGMSLAFQPMTPLLYLFA
                     RVDAGAAGELSGAESRELAELQAQFAELFPRRGGANLMLFRLSRASPPTARSLRLPVD
                     DVLRIERD"
BASE COUNT         1575 a         2668 c         2465 g         1459 t
ORIGIN      
        1 agcgtacacg cagaccagcg ccggctgggt ggtacagacg acggccatgc acggcgtgtg
       61 gcaaccgctc agcggcggct gtcacttcat ttacgattcc gggatgggcg cgctggacaa
      121 catcccgagc ggcaacaaac atcggctcgt cgccgctgcc tatcagctct acgtcacggg
      181 gcgcgtctat cgcaaggtga gaacgaggct gtactacgac gtcggaccct gctgacgaga
      241 ccaaatcaag ggcgcaatcc gtagcgtagc gtgaccaaga acgcgagccc gccgccgcac
      301 caaatcctcg cagcgcgcca ggcagccatt cgcgcgccga gcatcgcaac gagagcgccc
      361 gtcattccgc gttcgctggc gggcgctctc gacgcaaggt gtcccgagtt ccccgccctg
      421 ctgcgagccg agatcctgcg gctgcgaggc acggcctcgt ccttctccgc tcctcgaaag
      481 gcttcaacct tcctccggtg ctcactcctc gggcgccctt ctcgctcgcg tttcttggct
      541 ctcgcgacga gactcacttt ccagcggtcg gagcatcgta ctcgggccag cccgacggct
      601 cgagcgaacg catgtatgca acgaggcccg acttttccgc gtcggtgacc ttccacgtgt
      661 gattcttgcc ctgaaggagg tacgcgaact cgctgttgtc cttgatgggg cgaccctccg
      721 gatccacgcc ggtgagaaac gtattctgga tctcggtgtc tgggtcggcg tccacctccg
      781 tcggtgtatg atcgggtccc gcattcttcg agtgacaggc aatgcactcg gggttgttcg
      841 cgtccgcggc agtcgtgtaa cgcgtgtgac cggcgtccca ctgctgagtc gtgtacgaat
      901 agaccttcaa cggaacggtt gatttctgcg cgcccgaggt cgcggtgatc gtggtttcac
      961 ccgccttcag cgacgtgatc gtaacgtgct cgccgtcatc tgcgggagca agcgaagcga
     1021 ttgaagcatc cgcgatggtc cacgttaccg caccggactt gttcaccgcg atcgctggcg
     1081 ccttgtaggt gtgcatcccg tcgaatccgc tgaacaattg cggaggaaac acctctagct
     1141 ccttcggcga cgaatccgac ttgctgtcag acgagcaacc cgccgcgcag atcagcacca
     1201 aaccagcaac cacacttttc atggctaatt cctccttctc ggtggcgcgt cgatcaccct
     1261 gagaacgacc accaaagcgc tcgaggcgtt cgaactccga gctgtcttta ccagctagat
     1321 aaagaaacgt gaagagtcac cctgcgcaaa acacgtaatt tagcttcatg aaattgattc
     1381 attgcaacag cattgcgcac tcgcgattca gatatggaaa ctgcgagcga atctcctcgc
     1441 cgcgcacgca ccgtaacgac gcatcagcaa ttcactcctg cgcaattcgg cgcgtgcggc
     1501 tgctcacgcc gaacgcgctt cgatccggaa aagccgcgag ccgcgcctca cagctcgggc
     1561 agcgctgcga tccaatcgcg gatcacggcg accgcttcct cgtcgacctt gttggtcgcg
     1621 atcggcggca tctgctccgg aacgagctcg tcgtcgtcgc cgcgctgctc catacgatag
     1681 agcacggcgc tgctctctgg gtgccccggc gcgatccgaa tcgtgtattc gggcggaggc
     1741 gctagccagt cgcgcgaaat gcgctgacca accgtcgttt ggtagagctc cgtctcttcg
     1801 aatggcagct ctgcctcctc ggtccggagc cgaagcctca tcttcggggc cattcccttg
     1861 tccgtatgac agtgaccgca attcgcgtgg agaatcccaa gcgctttcga ttgggttgcg
     1921 tccccgggga tcgcgaagga atggtccggg gcgttcgtca ccgcaccgag ctcgaccaac
     1981 ctggatagcc aaccagactt cgacgtctgc atcgccgaga agccgagcac cgttccgggc
     2041 tcgcccttgt ggcagtgcat gcaaaacttt tgcgctggca cgtcgtgcgt cgtgccgttg
     2101 acgttcggca ccccgtcgct cgtgagaaca gcatcggact gatcatcgcg ccacacgaag
     2161 gtgcccagac ggtagtcttc cttctggacg ccagaatccc cgacccgttc gatcacgcgt
     2221 gtctcgagtc gcttcccttc caagctgaat tccttcaccg ccaccgtgcc cacggggaac
     2281 tgccagtggt ccatatcgct cgtgtcgatc tgcgcgcccg gaggaagaat cagccatctc
     2341 cgcttgacgg ctccgtcaga ccacagagcg tagtccggct cgtattcgag cgcgttttct
     2401 ggcggcgtcc cgctcgccaa gtcagcgtaa aaaccgcgat tcgatagcag atcggcgtcg
     2461 agcttaccgg tgacgtcgtc gtaaggagga tctccgtcgg cgcacgcgga caacgccgaa
     2521 cacatggcga gcacgagcga cagacgtgtc gtccacgcga gcatcaacgc agttcggttc
     2581 gtcctcgtca ccgcagcggg cgcagactaa ctcaaacacc gatcgtcgga gacggttatt
     2641 tcgtgagcga tatacagaga ccacgagagt gaataattgc gaatcagttc gaaggttgct
     2701 cgttcgccaa caccgcaaca atcgacgcaa gcgggacggc gataagactc gattcaagaa
     2761 aacttaatcg cgcgcaaata taattcatgt ccgtcgcgag cccccgcgga gttccgtcgc
     2821 cagccgagct ttcgcgctgc gtgtcgccgg ccacctccgg cgagaccgcc ccatagtatc
     2881 ctgactcgat acactcgccg ggagactagc ggcgtcccca gcttcggccg gtaagggccc
     2941 gagaatggat gagtcagtcg cgagcgagcc gagaccgccg cgaggcgggc gcgtgaggag
     3001 agagcaccgg acgcctacaa aagtctcagg attatcggat ctccgaacca cgcatcggcc
     3061 cgccgttttc ggggtttccg gggccacgaa agccacacga accaaggcat cggaacgaac
     3121 tctttcgaag cacgggtcgg tacgaactct ttcgtgccga gccctctgcg agcgaacgag
     3181 acgaaggcct gcctgctcgc gacatgtgac gaatcctgag accagagtag gcggaggagc
     3241 gcggcgacga gcccgtccgc ggcagcagga ggtcggctcg cgcaggatga gttacccgtt
     3301 tgcgggccat tagccgcaac taccgcgaag tcgtaacgcc gaacttcgcg tgctcggcgt
     3361 tctcgaatgg cggcgggcgg atcttcaggc tgagattttc ggaattggct tctgacagtt
     3421 gcaaccgagg gaacccacat cgggcgcgct cctcgtgcat gcccgcgctg gtccacgtcg
     3481 acggcgtgga gcgccgttca tccgtttgcg tcagtcgcaa tcgtgatcat ggccgtgacc
     3541 gtgacccggc cggcggcctt cgcaaagcgt agcggcgtag cttccgctcg cctggcccgt
     3601 agcaaagctg agtgtgccca tcgccacgaa ctgtccgctc gtagccttgt agcgctgagt
     3661 ccccgtttcg atcgtcgccg tggtcacgaa gtcgaccgga tcggtcgggg cggggccggt
     3721 aaagtgcaga acgctgacgt cgttcgtcat catcactccg cgagacgtcg tgatgacgct
     3781 cttgcccgaa gcgatgatcg tgttggggtc atctgggctc ggctcggtgg agtagacggt
     3841 gaagtcgtag ctcgcgttgt ccatcggccc gcccaagatg ccgtgggtac agagcccgac
     3901 tggggatgtg cacggcggaa cgggcaccgt cacggacgaa aacggccccg tgatttcctt
     3961 gcaccgcccg tggcggctgt ccgcttgaac ttgcgacgag agcgccgcga tcaggcccgc
     4021 actgaccaac ccggatacaa acgttcgacc gagcgaaatc gacatgctcc gaagcctcct
     4081 cttagaccta agagtcttca cgagtttttt cgaatgtcaa atcactcttc gaagttactt
     4141 cacgcttcga tgtgacgaga acctcgttgc accacgctca gctcttgtcg agagtgctcc
     4201 gaagctccgc gagcgacgaa cgcatcgctt cagaactcag agcacggccc gtcgcacgtg
     4261 ggaggatctc cgagaacatg cgggccgcct cgtcgaggtc gcacactttg tgaggatagg
     4321 cctgtcgagc caacaatgtg agcccagtca cgacgctgcg aacggacgcc gcacggaacc
     4381 cagagccctc gaaaatcacg gcggagcact tgatgaacga gctcgcgtca ccgagaaagg
     4441 cggcgatctc cttccgcaca gcgctgactg gaaggggcgc gccactctcg acgatcgtga
     4501 ggagcccgat ccctttcggg tggtgttgcg caaatgccgt gcatgccgac cgcagatgcc
     4561 ccgctccttc caacgtcgtt tcacggcgcc agatcaccgc gaagacctgc tgccacgttg
     4621 caagcgcgta gtcggggcta gttgcaacgg ttcgccatga ttcgatgatg ggcagcatgc
     4681 tcacgtttgc tttctcgagg tagactagaa cccgggcaaa ttaccgcgcg ctttcggcaa
     4741 cgtaaagccc ctgggcattg caccctcgag cagcccctgt ttcaggatgg tagcgggaag
     4801 acatcgcact acacccgcaa acagagtggt tgcagaccag ccttcaccca agaagccgag
     4861 ctcgccaatt gagagaacgg gcgccgctgc gctcgaaggc ttttgcaccg ctccttggcc
     4921 gtcagaacgc tccgggtaga acgacctgag aaagcgcctg accaaggccc aaggcgggcc
     4981 cgagccatgt gcgaggcgcg aggcgggctc atggccgtgc taccagcatg ggaagcgcgc
     5041 tcagcccggc agttggcgta ctcgcgtggg aggggctcgt cgcgttccaa ttcgacgccg
     5101 gaagacactg aactcggtgc gaagaccttt gcacagggac aaggaactcg cgctttggcg
     5161 cacgcctacg gggttgaact ttgagacaag ctcgagggct ggttcacaca cgttacgcaa
     5221 aacacgtgct cgagcagctc tggcaacagc tccaccggca agatcgtcat gaacgctctc
     5281 cccaagtccg tgacggaccg cttgctggct tcggggtctt cgctctcgtc cgcgaagtag
     5341 gtgaattttc gcttgccgag tccgccgaac caacgccgtg ctgcggcgag caatgagtcg
     5401 tacgcgtctt caccgccatc gacgagaggg aatagtcgcg ccatatcgag caagccgaac
     5461 aggtgaaccc cggcgttcac ggcgtcgagc actgcgcccg caaccggaga cccagccttg
     5521 gtccgcgcga tgaggacgac gcgtgcgcgc atgaggcccg ctgcctccca cgcttcgatg
     5581 ctcgcgccta tgccgagatc ggaagcagaa aggcctgtcg cctcgatgta gatttgagac
     5641 cgaagctggt ggatgcggtt cttgagcgca gagatttcca gcgctcgggc ttgactgact
     5701 tcgtagtcga gctcggagga gtcaccttgc gcgcagtcga cctccagcgc gcgaaagcgc
     5761 cagatgctcg ctctaccctg agcgcgatag cgctcggcaa cgtcgtagaa aatgaggcga
     5821 ctgaagcgtg ccacctcctg cacgtaagta acgagccagc gcgcatcggg acggctctga
     5881 acgtgctcgt acgcgtgcgc gtacaaatcg agcaggagac cgtcgtccgc ggccgagagc
     5941 ggcctcatct ccggcaatcg acaaaactgg tagtaaatcc agcttccgtg ccagaggctc
     6001 gtctggtgag ccacagactc ggttcgtcgc ttgctcgacg aagcgaagaa cgcggcgacg
     6061 tctggcgcac cgcgaaagtg ctcctggctc tgctcgaacg cggctcgctc gtcgacgaat
     6121 tcgcgctcgc tcttacccga caagtggaag tagcccgagt ccacgtacac cttccagaag
     6181 gtcgaagcgt cgggttcgcc gcgcgtcgtc cgtgggtgca gcagctcctc cagctcgagg
     6241 tgccagcgcg acgcctcggc tccccgaagc agcaagcgaa gccccacccg atgcaacgcc
     6301 gaactcgact gttgcgtcac gtgcgcgacg actgcctcgg ctttgatcgg ctgcccgcct
     6361 ttccatcgca gctccaccga tttcagccgg agccccgggt agagcatgtc tcgcacggcg
     6421 tcggtttcga tgcagactcc ttgctcggac acgtccgtgg cgcgtgcgtc aatcacgatt
     6481 tcgcgccaga gcgggtgcgg cgccgtgagc cgcaaccgac gaggcgcagc cacgcgtcga
     6541 aagcggcgcc gccgaacacg caccatcgtc gtgggcggca gcagcgcatt gcgcacgggg
     6601 acgtcgaaca agtacacgga cgagaaacct tggacctgca cctcgatcgc cgagccttga
     6661 aggctcgcgt ctggcggcac gctccacaag aaatttcctt cgcgcgtcag gcgcaactcg
     6721 acggtccggc gccgctcgcc tctcgcgctg ccatggagca tcgcgtgacc gcgggcttca
     6781 ctcgctatca gcgccgaaac gatcccgcgg atgcgggcgg catcgtcgat cagctcgacc
     6841 tcctcctcgc gctcatcgat tcggaagatc gtaccttcca ccagccggtc gttgacccac
     6901 gccaaggagt gcctggccgt gttcaagccg agcgcctcga actgaatccg gacggcggcc
     6961 aactggctcc cggtcggagc acgcgcctgt tcgccggtca cccgggacag gccggggagg
     7021 caaggatcga agaccgaact gtcaccaacc agcagatgcg aacgcaagaa tccgtccaac
     7081 gcgagccggg agcccgattc cggctccgcg cttgtcacgc tcctcgtgat ggaagtcact
     7141 gggcgccgtg tcagtcgaac gctccttggt gaaccgcgtc ggcgctccag tcgctcgcgc
     7201 gatgcgccgg aagatggccg cccagcccgg cggtgaggct acttactttg gcggacatag
     7261 ctcgagatgt aaggaagtcc atccgtcggg taggtcgtcc gaacgcacat catcctcttt
     7321 ccaggtccgc aagcgccaag caacggctcc gacgatcgta tgggacgcga gcgtcagctc
     7381 tacaagcggc cagctcacga ctcaattaac accgcggagt gagcaatttg cgacgcttgc
     7441 aaggcgaaac cacccatatt tcgggatttc cgttcgggcg cggggtcaat cacgctcgat
     7501 ccgcagcacg tcgtcgaccg gtagccgcag ggagcgggcg gtcggaggcg acgcacgcga
     7561 gaggcggaac agcatcaaat tcgcccctcc gcgccgcgga aacagctctg cgaactgcgc
     7621 ttgaagctcc gcgagttcgc gactttcggc cccactcaac tcccccgccg cgcccgcgtc
     7681 gactcgcgcg aacaagtaga gcaagggcgt catcggctga aacgcgagcg acatccccgt
     7741 gctgcgcaac cacacccgct ccagtgcgcg accgccgtga aaccaatcgc tctttcctct
     7801 cccgggagcg gtcaggagcc ccaccgccga cgcggaggcg acggccttac ggctgggtcg
     7861 ctcgagcccg cgcccaagcc caagcttgcc gacgaggtcc attagcgtcc aggtggagac
     7921 gaggcgcatc cccgccaagt cggtaggcgt cagctccagc gtcgccacat cgaggccatc
     7981 ccgagtggag gcaacgtcac gcgcgttcca tcgaacctcg tccatcattt cgcgatgcat
     8041 acgcccgttc aaaaggcgca ggcgctcgcc gcgcgcgatg atctcaccga ttctgtcgag
     8101 ctcgtcggcg tcgacgagca ggtccaggtt ggccccggcg gcgcgggctt cggtagcgag
     8161 tgcgtcg
//
LOCUS       DASTKU010000125         5489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_915025, whole genome shotgun sequence.
ACCESSION   DASTKU010000125 DASTKU010000000
VERSION     DASTKU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915025"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VFQ61_04505"
     CDS             <1..605
                     /locus_tag="VFQ61_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET9953734.1"
                     /translation="YLTAEVRADALIATRQTLNEKLAARGEKLSLNDLLIRACASALR
                     RVPAVNASFMSDSIWLHQVIDISVAVALPEGLVTPVLRDADKKGLLEISREVRELANS
                     ARQKKLKPEQMSGGTFSISNLGMYGIDEFQAVINPPEGAILAVGAIREVPTFSDGAVR
                     PENRIRLTLSCDHRVIDGAVGAQWLAALRELVESPLELLL"
     gene            complement(743..1249)
                     /locus_tag="VFQ61_04510"
     CDS             complement(743..1249)
                     /locus_tag="VFQ61_04510"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9953735.1"
                     /translation="MRRAQGTIHVFTFKEGVFSAVAHDLRIRLDRFDISLEGQAVRAE
                     LDLTSLAVEGPMENGVLQANAYDAGKRADVEKAMHGDVLRTKQHPKAVYAGTAAPSGS
                     GFRVQGQLELAGQQAPLAFDVTNDNGTYRASFELKPSQFGIAQYKALLGAIRLKDVVR
                     VELALQEA"
     gene            complement(2426..2995)
                     /locus_tag="VFQ61_04515"
     CDS             complement(2426..2995)
                     /locus_tag="VFQ61_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9953736.1"
                     /translation="MRIGEVAERLGVRTSTIRFYEKRGLLPEPARIGGKRSYGAEVLR
                     RLHLIDICQKGGFSLAEIETLLRHFDAEETELAQALLLKKIAELDAIVERAKCVREVL
                     LESFACGAGAGGLEGPASELRPRPVSEALAGDVRGIELRTFDARGALEARGLEPRGVH
                     DTRGTIDLRRGVDLVNTAIEFREEGRAEG"
     gene            5161..>5489
                     /locus_tag="VFQ61_04520"
     CDS             5161..>5489
                     /locus_tag="VFQ61_04520"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HET9953737.1"
                     /translation="MNEEASEDAGPGTGKRHESGLHAVHFYEDEKLLVEMVAEFIGGG
                     LRAGTGALIVATQLHLAAFTEELKRQVDMDALGGRLILLDAEQTLSRISAEGIPDAQL
                     FNSFFDEA"
BASE COUNT         1022 a         1754 c         1587 g         1126 t
ORIGIN      
        1 tctatctcac cgcggaagtg cgagccgacg ctttgatcgc gacgaggcag acattgaacg
       61 agaaactcgc agcgcgtggc gagaagctct cgctcaacga cttgctgatt cgcgcctgtg
      121 cgagtgctct gcgtcgcgtg ccggcagtga atgcctcttt catgagcgac tcgatctggc
      181 ttcatcaagt aatcgacatc tccgtagccg tagcgctgcc ggaaggactg gtcacccctg
      241 tgttgcgcga cgccgacaag aaggggcttc tcgagatctc tcgcgaggtc cgtgaattgg
      301 cgaactccgc acggcagaag aagctcaaac ccgagcaaat gtcgggtggt accttttcga
      361 tctccaacct gggcatgtac ggaatcgacg agttccaggc cgtcatcaac ccaccggaag
      421 gtgcgatctt ggcggttgga gcgatccgcg aagttccaac cttcagtgac ggcgcggtac
      481 gcccggagaa tcgcatccgt cttacgctat cttgcgatca tcgtgtaatc gacggagccg
      541 taggcgcgca atggctggcc gcgctgcggg aattggtcga gtcccccctg gagctgctgc
      601 tctagtgcag ggccggagac tgttctcgct caaccttgcg ctccaccaac gatcgcgcgc
      661 tctcttctcg ggccatggac accccgcacg taaggggccc gccgcatcag tgccggtgta
      721 ggccccttgc tcgagtggcc gctcaggcct cctgaagggc cagctcgacg cgcacgacat
      781 ccttgagacg gatcgcgccc agcagcgcct tgtactgggc gatcccaaac tggcttggtt
      841 tcagctcgaa gcttgcccga taggttccat tatcgttcgt gacatcgaaa gccagcggag
      901 cctgctgacc ggccagctcc agttgcccct gtacccggaa accagagcca ctgggagcag
      961 ccgtgcccgc gtagaccgct ttcgggtgtt gcttggtgcg caggacgtca ccatgcatcg
     1021 ctttttctac gtctgcgcgc ttgccggcgt cgtacgcgtt ggcctgcagc actccgtttt
     1081 ccatcggccc ttccacggca aggctcgtca ggtccagctc ggcgcgaacg gcctgcccct
     1141 ccaggctgat gtcgaaccga tccaatcgaa ttctcagatc gtgggcaacc gcggaaaaaa
     1201 ccccttcctt gaacgtgaat acgtggatgg taccttgagc tcgtcgcatc ggataaagct
     1261 aatcggtccg gggccgattg ggaaaggcga attgaccacc acgcaatgag tttcatgagc
     1321 cccgattgtt gcagtgagag agcggcgcga acgctggctc ggtgactaat cagagaccgc
     1381 gccggcggtc accacgttcg ccaacggact gcagcgccgg tgatgtcgtc gctatctcac
     1441 atacgctgca ctggtcacga catggagctc gtatcgtagg atatcctcat tgcgatgatc
     1501 cgccttctga cgtcgaaaca ctcgagttct gcacactggc tccttcgcag ccaattcggg
     1561 gttatcgact attcaatcga acccttgcgg gtaggcacac gcatttaccc gctcgcccca
     1621 cggcgacacc tgcagctccc atccaaacgg ctatgctcac gcccaaacct gcattccggt
     1681 tcgtgcgtag actcgacacg tggtgaatgc cttcgctcac gggcttgaac cacggggacc
     1741 tcacggccta gacgcctcgg ttgctccccg cgcatggggc tctggagccc attgccgtac
     1801 gagcacagga gatgacggcc aagaggttga gtggctccgg tatgaattcc gcgatgcgca
     1861 accctagccc cgattgttgc gggcgcgcgc agatcagcat gccttccacg acgccgcatc
     1921 acgcgcgcga tcccgaccaa ctttcctagt ggaggggcgc tagggcgcgc cggttcgcgg
     1981 aggcacggcc tatcaccctc cgctcgcgcc atgcgacgaa tccggaagag cggagctggc
     2041 taaaggacta gcgagttgac tagctacctg attggaccta actccgctgc tgaagcccct
     2101 gaacacgctc cgctgctgaa tgcgctccgc atgcgctccg catgcgctcc gctgctgaac
     2161 acgctccgct gctgaacacg ctccgctgtg gaatgcgctc cgctgctgaa cgcgctccgc
     2221 tgtggaatgc gctccgctgt ggaatgcgct ccgctgtgga atgcgctccg ctgctgaacg
     2281 cgctccgctg tggaacgcgc tccgctgtgg aatgcggagt tgtgaggggg cgcgccgccc
     2341 aacgtggaag ctggtccagc actaggctag cgccaggatc cagcttgcac gatgagtagc
     2401 gcgaccttcg ccactcgggc cgctgttagc cctctgccct gccttcttcg cgaaactcta
     2461 tggcagtatt gactagatcg acgccccggc gcaggtcgat ggtgccgcgc gtatcgtgaa
     2521 caccgcgcgg ctctaaaccc cgagcctcca aggcgccacg tgcatcgaat gtgcgcagct
     2581 caatgccacg gacatccccc gcaagtgctt cactcacggg gcgcggccgt agttccgacg
     2641 cgggcccttc gagacctccg gcacccgcgc cgcatgcaaa actctcgaga agtacctcgc
     2701 gaacacactt ggcccgctcg acgatagcgt ccaactccgc gatcttcttg agaagcaacg
     2761 cttgcgccag ctccgtttcc tctgcatcga agtggcgcaa cagagtttca atctctgcta
     2821 acgaaaagcc acccttttga cagatatcga tcaaatgcaa ccgacgcaac acctccgcgc
     2881 cataacttcg ctttcccccg attcgcgccg gttcaggcaa taagccgcgc ttttcgtaaa
     2941 agcgtatcgt ggaagtgcga acgcccagtc gctccgcaac ctcgccaatc ctcattcggc
     3001 atcctctcat gttccgtcag gacgaagttc tgacggctgc cggagtatgc catgtcgtcc
     3061 agcaatcgtc atctagagtt cgtctcatcc aaggcagtat tctcgctctg cctacttttc
     3121 tgaatgggcc cgtgtgtgcc cgctgtgtgc gcaagcccgt tcgagtcagc ccgggcttcc
     3181 aaatcctcga gtcggatcgc accctcgtgg ggggtgcgca cgagggtcga cagcgggtgt
     3241 ctgtcgttcg cgctctgccg aattgctggc gatgagcccg ctgctccggc gccgaagcgg
     3301 tagaaggtgc gggcaatgag cactccatta gagctgcagc caccgagctc ttctcggttt
     3361 gcaagcaacg ctcggtttgc aagcaacgct cggtttgcga gcccagcttg cgtctgaggc
     3421 actatcgaga ccaaaagttt cttgttcggt tttctcggca gccgtggctc tatcagtctc
     3481 accgcagggt tgattgcgct gttggcattc accttgtctt gcgttcacct tgttcaccgc
     3541 gcgctgctgc cgtcctcgcg tcattcacga tcgttctggc tggacctttt ccggctcggt
     3601 tccgtgggct ttcgtcgtct ggcactcgac ccgccactcc agtcttgacg gggtgcgagg
     3661 cctaccctga cgctttgggt gctattggat cgacacaatc acgggtgcga gtgctctcat
     3721 cggtgcatac gacactcgac cccgcgctcg gttgctcgcg ccatcgattc ggccgcatcg
     3781 cgtcagtatg tcggcaccgc tctttccaac cgcatcgtct cctcaacgtc attcaattcg
     3841 accatcgggt ccgtccacct gcgcagttag cttcgcgctc cgtcaacttc ccttccggta
     3901 cgttctctcg ccgggcggca gaatcggttt ggtgagtcgg ttcagaaagc atgcgctcgg
     3961 tgatcccaag tcaggttcgg ctgctgggta ggtgcgatgc tcgtcacgcg gcgctcaccg
     4021 cccgagtcga gcagcatggc gaagcagcag attctccgag agattggttt ctagggttgt
     4081 gtcgtggaat cgccagctcc tatgtgaata tctctcgaga tatcctcatc tcagcacctc
     4141 gaaaccaagg gcggaccgtc gcgagcgtga gcccttagga cgcgaggcag agcggggctc
     4201 tctattcaga aaccttggaa acaaccgctc ttcccgcgac tacgccgcta gcctgcgaaa
     4261 cggaaaccca ttacctgaat ggaaccccga gcaaggcagc tgggcagccg gctacagacc
     4321 tcctgcatac ctccttagag ttctaggtta accaaagctt cgaccgattt gatcacggag
     4381 cacacttttt ttccgatacg ggcgttttct tgaaggcctc tcgggactct accgtgctcg
     4441 cgcgctcgcc gcgtgggcct ggaagccatc gcgcttattg tcgacgctcc gatgggccga
     4501 tggcacgcgg cgttgctcac gctgaagtgg ccactgcgtg aggccaatac tcctcacatc
     4561 cggcaactca accggcagag tgatgattgt cgatcgggcc actgtggcag caggctcggc
     4621 tgcagggcga atcggcccga gataccagct gagagcccca ttagcccgga gggcccccgt
     4681 ttgcggcgca gatggcccag atccggctca gggggccagc aaccagacgc tcgctcgaac
     4741 tcacttgctc ccaacagcag ccacatggct aggtatcgag caaactaggt atctggtcgc
     4801 cggtatctgg tcgcgtggaa cgcggagatc cgtggctatc gcgggcgaag catcagcggc
     4861 cgcgaacccg ctgctccggt ttggcggtat tccgggaggg gctcacgcat tgaggcaact
     4921 tgtctcgcga cgggcgctgc gaagcttgga agtgccaagc aggggaacac caatttcctt
     4981 aggcggtaca aggattgcag aggatcacgc gaagccgcca agcatcaaaa tcgtacgttt
     5041 atcgcatatt ccgggtgggc ttcacaagga gaacctgccc catgtgacgt ttctgctgca
     5101 ccaatgacgc tcccgcccag tctgctgcgc tcgccccggt caccccattc attgcactca
     5161 ttgaacgagg aagcgtcgga ggatgccgga ccgggcacgg gcaagcgcca tgagtccggc
     5221 ttgcacgccg tccatttcta cgaggacgag aagctgctcg ttgaaatggt cgcagagttc
     5281 ataggcggcg gccttcgcgc tgggaccggg gctctgatcg tcgcaacgca attgcatctc
     5341 gccgcgttca ccgaggagct gaagcgccag gtggatatgg atgcgcttgg aggacgtctc
     5401 atcctgctcg acgccgagca aacgttgtcc cgcatcagcg cggaaggcat cccggacgcg
     5461 cagctattca acagcttctt cgacgaggc
//
LOCUS       DASTKU010000126         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_245372, whole genome shotgun sequence.
ACCESSION   DASTKU010000126 DASTKU010000000
VERSION     DASTKU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245372"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VFQ61_04525"
     CDS             complement(<1..738)
                     /locus_tag="VFQ61_04525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET9953738.1"
                     /translation="MAPRADAAHSILETNAPRLSRGEPLCPARTLPDHGACIPVPSGL
                     EEEEGAPQLEIEQNAHHDRRGHWVSYEHVPKRPDRPSEYRSYRFPIAVQKNQSFVMSG
                     YDLDRPDEAQRRGSMKAVGHGGVDLAAPRGTPIHAIQFEHQLADSEVLFVGELFGTSV
                     VLLHNVREAGIDREYLSLHGHLERAASGLKRGDVVAPGTLIGFVGDSASRGIVHLHYE
                     VRRARDGVKLRELGPGELAHNSRTLACD"
     gene            1033..1851
                     /locus_tag="VFQ61_04530"
     CDS             1033..1851
                     /locus_tag="VFQ61_04530"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9953739.1"
                     /translation="MPFPYKLGPADAAIFETFVVPRYLSLFGELAMEMLLSGPSARLL
                     HIGCRTGYPDLKLCELIDQPEILSLDVSLPALELARHKAAVLGQTSIEYRFAEWLPSQ
                     ADAGMFTHVLSLHPLLPDPARVDLEAAMRWLLCSGGQALLAVPLRGSFQEVLDLLREY
                     ALKHDEGSFANLLEGVLSERPTIETLSQEFEDAGFEDIDVEVRNVVLPFESGRAFMED
                     PVTRLMIMPELATWLGNQDLRTPLEYVREAMDLYWSEGTLELNVNVGCVSARCP"
     gene            complement(1887..>3055)
                     /locus_tag="VFQ61_04535"
     CDS             complement(1887..>3055)
                     /locus_tag="VFQ61_04535"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET9953740.1"
                     /translation="EFELTSLGLKLSDVDLAARAESEGSETRITFDRLTGHTGEQKRV
                     VRVQRGRLWLKGLEFNRADGAIDASELPLVIQGIPQAIATTRQSIAFTVERTPQEIRA
                     QFQIPYLIVALPQSLGRNVIPLDENRSVTVLQPLAAPKLAQGDTLPFRLSFDLGQNVK
                     LTRSDLDLPLSGQAEVRLADEVDVHGDLDLSPGGRIDVSGKTFVIDSGEVHFDTGEPN
                     NPRIRVTASYRAPDGSIVTADMAGTLKKANLRVSSPGRTEQEAYAILLGSSTSVDDSS
                     AAGARAAGAGVGADRLLGPLLQNTPLHRVELRTGSERVANQRTYSTYTAAVPVAEDIW
                     FEGSYKSLNRTNVNNDTVSAWSGTVDWRFRRNWSLRTEVGTIGTGMDVLWNYRY"
BASE COUNT          531 a          947 c          975 g          602 t
ORIGIN      
        1 atcgcaggcg agcgtccgcg agttgtgggc gagctcaccc ggacctagct cgcgcaactt
       61 cacgccgtcc cgcgcccgtc gcacctcgta gtggaggtgc acgatgccgc ggcttgcgct
      121 gtcacccacg aagccgatca gcgtgccggg ggcgacgacg tcaccgcgtt tcagcccgga
      181 tgctgcacgc tccaggtgcc cgtgcaacga cagatactcg cgatcgatac ctgcctcacg
      241 cacgttgtgc aggagcacca cgctcgtccc gaacagttcg cccacgaaca acacctccga
      301 gtctgccagc tgatgctcga attgaatggc gtgaatcggc gttccgcgcg gcgccgccag
      361 gtccacccca ccatgcccaa ccgccttcat gctgccacga cgctgggctt cgtcgggacg
      421 atccaaatcg taaccgctca tgacgaacga ttggttcttt tgcaccgcga tggggaagcg
      481 ataggagcga tactccgacg ggcgatcggg acgcttcgga acgtgttcgt aactcaccca
      541 atggccacgt cgatcgtggt gcgcgttctg ttcgatttcg agctgcggcg cgccctcttc
      601 ctcctccaaa ccgctcggga cgggaatgca cgcgccgtga tcggggagcg ttcgcgcggg
      661 acagagcggc tcgccgcgcg agagccgtgg cgcgttcgtc tccaaaatgg aatgcgccgc
      721 gtccgctcga ggcgccatga ctggaggaat tcggccttgc gcaactcgac tcaggaccag
      781 acaggtccct gccagagcgg cgagccggac gagcggatgc atgagcagcg ggaagcctaa
      841 cgcggcacgt agcacacgga ttcccgaccc caacaaaaca gaggaagtac gctgaaatcg
      901 accacagagc gggtacctcg cgcggagtgc gatgataggc tctcgccgcc tcgagctctc
      961 tccggggacc cgcaacgtct tctctcgacg gcctctcccg agtctgtttt ccgaacctgc
     1021 tttctccgag ccgtgccttt tccgtacaaa cttggccccg ccgacgcggc gatcttcgag
     1081 accttcgtgg tgcctcgcta cctctcgttg ttcggcgagc tcgcgatgga gatgctgctc
     1141 tccggaccat ctgcgcgctt gttgcacatc ggttgcagga ctggttaccc cgatctgaag
     1201 ctgtgcgaac tgatcgatca gcccgaaata ttgagcttgg acgtgtcgct gccggcgctg
     1261 gagctcgccc ggcacaaggc ggccgtgctc ggacagacgt cgattgagta ccggttcgcg
     1321 gaatggctgc cgagtcaagc cgatgcgggc atgttcacgc acgtcctgag cttgcatccg
     1381 ctgttgcccg acccggcgcg ggtggatcta gaagcggcaa tgcgctggct gctttgctcg
     1441 ggcgggcagg cgttgctcgc ggttccgctt cgcgggtcgt tccaagaggt gctggatttg
     1501 ttgcgtgagt acgcgctcaa gcacgacgag ggcagcttcg cgaacctgct cgaaggcgtg
     1561 ttgagcgagc gaccgacgat cgagacgctg agtcaggaat tcgaagacgc gggcttcgaa
     1621 gacatcgatg tggaagtgcg taacgtggtg ctgccgttcg agagtggtcg ggcgttcatg
     1681 gaagacccgg tgacgcgctt gatgatcatg ccggagctgg cgacctggct cggtaatcag
     1741 gacctccgaa ctccgctcga gtacgtgcgt gaagccatgg atctctattg gtccgagggc
     1801 actctagagc tcaacgtcaa cgtcggttgt gtcagcgcgc gttgcccgtg agccgctcgc
     1861 tacggccgcg tagctcctga tgggcctcaa tatcggtaat tccagagcac gtccatgccg
     1921 gtgccgatcg tgccgacttc ggtgcgcaga gaccagttcc ggcggaagcg ccagtctacg
     1981 gtaccgctcc aggcggatac ggtgtcgttg ttgacgttcg tgcgattcag agacttgtag
     2041 ctgccttcga accagatgtc ttctgcgacg ggaacggccg cggtgtaggt cgagtacgtt
     2101 ctttgattgg cgacgcgctc gcttccggtg cggagctcga cacgatggag cggggtgttt
     2161 tgaagtaggg gaccgagtag acgatctgcg cctacgccgg cccccgcggc gcgcgcgccc
     2221 gcggcactgc tatcgtccac gctcgtagag ctcccgagta gaattgcgta ggcttcctgt
     2281 tcggtccgac ctgggctcga gaccctcaag ttggccttct tgagcgtgcc cgccatatcc
     2341 gcggtgacga tgctgccgtc gggagcgcga tagctcgcgg tcactcgaat gcgcgggtta
     2401 ttcggctctc cggtgtcgaa gtgcacttct cccgagtcga tcacgaaggt cttgccggag
     2461 acgtcgatgc gtccccccgg gctcagatcc aggtcgccgt ggacgtctac ttcgtcggcg
     2521 aggcgcactt cggcttgacc gctcagcggc agatccaagt ccgagcgcgt caatttgacg
     2581 ttctgaccga ggtcgaacga cagcctgaac gggagcgtat cgccttgagc gagcttcggg
     2641 gccgcgagcg gctgcaatac ggtcacggac cgattctcgt ccaacggaat gacgttgcgt
     2701 ccaagcgact gaggtagagc cacgatcaag taggggattt gaaactgggc tcggatttcc
     2761 tgtggcgtgc gctcgaccgt gaacgcgatg ctctgtcgtg tggtagcgat ggcttgggga
     2821 atgccttgaa tgacgagggg gagctcactg gcgtcgatcg cgccgtccgc tcggttgaac
     2881 tcgagaccct tcaaccaaag cctgccgcgt tgcacgcgca cgacccgctt ctgctcgccg
     2941 gtgtgtcctg tcaaacgatc gaacgtgatg cgagtttccg agccctcgct ttcggcgcgc
     3001 gcggccaagt caacgtcact gagcttcagg cccaagctag tcaactcgaa ttcgc
//
LOCUS       DASTKU010000127         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_24807,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000127 DASTKU010000000
VERSION     DASTKU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24807"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFQ61_04540"
     CDS             <1..205
                     /locus_tag="VFQ61_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953741.1"
                     /translation="PRSTVQVRPYRPVLQYDSHHSMEARVELVLEIRHSTAYNEVVDQ
                     SESEILKSIVDKLHALGISQRGG"
     gene            334..909
                     /locus_tag="VFQ61_04545"
     CDS             334..909
                     /locus_tag="VFQ61_04545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9953742.1"
                     /translation="MLKLAIILGSTRPGRNGEAVAKWAFEHAAKRTDAAFELVDIAQF
                     ELPLLDEPTPPAMGKYTKPHTQAWAAKIDSFDGFVFVTPEYNHGTSGALKNALDFIYR
                     EWNNKAAGFVSYGSAGGTRAVEQLRQIMAELQIADVRAQVSFSLFTDFENFSTFKPDS
                     RHEKSLQGMLDQLIAWSRAMKALRAGQLPKD"
     gene            967..1623
                     /locus_tag="VFQ61_04550"
     CDS             967..1623
                     /locus_tag="VFQ61_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9953743.1"
                     /translation="MEPPFRLYDYALSGNGWKIRTLLRHLGYPFAIHWVDILAGEQHT
                     PWFVAKNPVAQIPVLEDSSGQVWTESNAILETMAEHTPLLPSAHARHEARAWMCFEQT
                     WVDGVISRARFRRRFPDLIPTSPEFFEVWRGEGARALRTLERHLTARHYLVGDSFSVA
                     DISLYAYVHVAHEAGFDLDEFPNLSMWLRRISREPGVLPVHVNPEALESRAPASEGAD
                     "
     gene            complement(1632..>2356)
                     /locus_tag="VFQ61_04555"
     CDS             complement(1632..>2356)
                     /locus_tag="VFQ61_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HET9953744.1"
                     /translation="IQSTAVHEVGHALGLPHEHQREDRVLCEKEQTIYDGCLACQDGS
                     CSAADYERCFLFPPTYDSNVLTEKDKGASASRVRDRTKKDSFKPLTEYDFFSVMNYCS
                     GRYVDFPDPIKSDWRLTSYDALGIEMLYPRSGQGAALELGCGKACFRTTPFGALIRSN
                     GSVRDEWTARGGKEWWLNTPVWQLPGGKTITANSLSASQLGASQGVGYDATVKWSGKV
                     VGGEGFVTVSDAKWTALVTTFI"
BASE COUNT          483 a          749 c          674 g          450 t
ORIGIN      
        1 cccgcgaagc accgtacaag ttcggccata tcggcccgtg ttgcaatacg actcgcatca
       61 ctccatggag gcacgggtcg agctggtgct cgaaattcgc cattccacgg cctacaacga
      121 ggtcgtcgac cagtcggaga gcgaaatcct gaagagcatc gtcgataagc tgcatgcgct
      181 cgggatttcc cagcgcggcg gctgacgctc ccgaacctca ggtggggctc gcgccacgaa
      241 cgcggtgagt attggtgaaa agccccaagt ggagctggta tgggacagct cgcaatgcat
      301 gccagcgcag aggaagtgag gtactgagag aagatgctca aactcgcgat catcctcggc
      361 agcacacgcc ccggacgcaa cggcgaggcc gttgccaagt gggctttcga acacgccgcg
      421 aaacgcacgg acgcggcctt cgaattggtg gacattgcac agttcgagtt gcccttactc
      481 gacgaaccca caccgcccgc gatgggcaag tacaccaagc cgcacacgca ggcctgggcc
      541 gccaagatcg attcgttcga cggcttcgtg ttcgtcaccc ccgagtacaa ccacggcacc
      601 tccggtgcgc tgaagaacgc gctggatttc atctatcgcg agtggaacaa caaagccgcc
      661 ggcttcgtca gctacggcag cgcgggcggc acacgcgcgg tcgagcagct ccgccagatc
      721 atggctgagc tccagatcgc ggatgttcgt gcccaggtat cgttctcgct cttcacggat
      781 ttcgaaaact tcagcacctt caagcccgat tcgcgccacg aaaaatcctt gcaaggcatg
      841 ctggaccagc tgatcgcctg gtcacgcgcg atgaaagcgc tgcgcgcggg gcaactgccg
      901 aaagactgag gcacaagcca gaaagcgctc tggaacgaga ggctcgttca tgcgagcctc
      961 tgcggagtgg agcctccctt tcgactctat gactacgcct tgtccgggaa tggttggaaa
     1021 atccgaaccc tcctgcgaca cctggggtat ccattcgcca tccactgggt ggacattctg
     1081 gccggcgagc aacatacgcc ttggttcgtc gccaagaacc ccgttgctca aatcccagta
     1141 ctcgaggatt cgagcggcca agtttggacc gagtcgaacg ccattctcga gacgatggcg
     1201 gaacacacgc cgctccttcc gtctgcgcat gctcgacacg aggcacgggc ttggatgtgc
     1261 ttcgaacaga cctgggtgga cggggtcatc tcgagggctc gcttccgacg gcggtttccc
     1321 gatctcattc caacgtctcc cgagtttttc gaggtgtggc gcggcgaggg agctcgggcg
     1381 cttcgcacgc tcgagcggca cctcaccgcg cggcactatc tcgtcggcga cagcttctcc
     1441 gtcgccgaca tctcacttta cgcctacgtc cacgtcgccc acgaagccgg cttcgacttg
     1501 gacgagttcc ccaatctctc gatgtggctg cgtcgcatct ctcgagaacc cggcgtgctg
     1561 ccggtgcatg tgaatccgga agcactcgag tcacgggcgc ccgcaagcga gggcgccgac
     1621 taacaccatc gttagatgaa ggtggtcacg agcgccgtcc acttggcatc gctcaccgtg
     1681 acgaatcctt cgccgccgac gactttcccg ctccatttga ccgtggcatc atatcccacg
     1741 ccttggctag cgccgagttg gctcgcactc aacgagttcg cggtaatggt cttacccccg
     1801 gggagctgcc aaacgggggt gttcaaccac cactctttac ctccgcgagc cgtccattca
     1861 tcacggacgg agccgttcga gcgaatgagc gctccaaacg gcgtggttct gaagcaggct
     1921 tttccgcagc ccagctccaa ggcagcgcct tggccacttc tcggatagag catctcaatg
     1981 cctagcgcgt cgtaactcgt gagacgccag tccgacttaa tcggatccgg gaaatccacg
     2041 taacgtcccg aacaatagtt catcacggaa aagaagtcgt actcggtcaa gggcttgaac
     2101 gagtccttct ttgtacgatc gcgaacacgt gacgcggacg cgcctttgtc cttctcggtg
     2161 aggacgtttg aatcataggt cggcgggaat aggaagcaac gctcgtagtc tgcggcggaa
     2221 cagctgccat cctgacacgc caagcaccca tcgtatatag tctgctcttt ttcgcacaac
     2281 acacgatcct ctcgctggtg ctcgtgcggc aagcccaggg cgtgccccac ttcatggaca
     2341 gcagtcgact ggatcg
//
LOCUS       DASTKU010000128        12045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_24972,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000128 DASTKU010000000
VERSION     DASTKU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12045
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24972"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VFQ61_04560"
     CDS             <1..494
                     /locus_tag="VFQ61_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET9953745.1"
                     /translation="RAYLSRMYRAHKRIWLTNSYFIPDRNVVRALVRAARRGVDVRVL
                     LPDKSDVRIADYASRAVWGKLLRNGVRIFTWTRSVLHSKTAVIDGTWSTIGTFNLDHL
                     SLRVNLEVNVSVLDEGFASEVEGVFIADLQRAREVDPKQFRFRPLAERCIELLAYRLR
                     KWM"
     gene            complement(636..1340)
                     /locus_tag="VFQ61_04565"
     CDS             complement(636..1340)
                     /locus_tag="VFQ61_04565"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9953746.1"
                     /translation="MSSIRTPEPSAPRAQAPLEILYEDEDLVIVNKPSGLLVHRGLAQ
                     DADTALFRVRDHFRQRVYPAHRLDRGTSGALLFARHPTAMSSLGKLFECGAVEKSYWA
                     LVRGRPPESGIIDHPVPRAEGAERVPAVTRFRLLATSPRERCSLVEALPVTGRFHQIR
                     RHLSHIHHPLVGDVNYGRGDINRKYRAEYGFHRLGLHAHRLAFTHPLRALNIVVEAPL
                     DPEFARVLDELQLSFR"
     gene            1394..1975
                     /locus_tag="VFQ61_04570"
     CDS             1394..1975
                     /locus_tag="VFQ61_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953747.1"
                     /translation="MNTSAENHHETIAFPAITLSELARKYEALWSLRQAGSGFDKASV
                     RAISAEFPGSLRELDVLPDEQLMARRAHARAACQGEAPEAWLIWLLAYHSEMRVVLEV
                     QRRIGELRSSRKALGMSVQPKPMIEQLMLDPLFEQGFAYTPEQLHSLLCPPGGRLNNV
                     VFGWLAERFGVDAERLEQTLFPRSEAGANHERG"
     gene            2022..3188
                     /locus_tag="VFQ61_04575"
     CDS             2022..3188
                     /locus_tag="VFQ61_04575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9953748.1"
                     /translation="MGAETLSGLQPTAGTPFLSFAWLDALERTGCVTEARGWLPMHMT
                     LHRAGSAPASKRTNSVGERVGGDHARGNNGELLAFAPAYVKTNSEGEFVFDHGIARFA
                     QTSLSLPYYPKLILAVPFTPVTGSRLLVAPGQDPSEITAVFAGGVRSFCEQQGLSGAH
                     VLFPPAAEASAWVTSGYARRIGVQYQWHNQGYGSFDEFLGTFNAKRRHQIRRERRQVA
                     EQDVEIEVVTGRDLEPSWADFIFDFYRSTVDKYFWGRRYLNRSFFEEVLTTLRDQVHL
                     VVARDRGSRRRLAGAFNLLGSDALYGRYWGAIEDRNCLHFEVCFYSGVEDAIARGLKR
                     FEPGAGGEHKLARGFVPTRTFSTHHYVDPRLELAVRDFLAREESAIEAELESVE"
     gene            complement(3201..3962)
                     /locus_tag="VFQ61_04580"
     CDS             complement(3201..3962)
                     /locus_tag="VFQ61_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953749.1"
                     /translation="MKLTPVARLMRVSARSTLTLLALTSSAPGAPESLGGADQAPPQS
                     NASDQPEREQHSRPRLASSVRGEIEADNERFDGDGVYGRFDGDVNLALGVGAEVSEVA
                     RATAVGRVLYYSSVGVFAAYSDAMDATAHVRRTAAIGLELRPLFLLRWSNDLEWGCAM
                     CDLTLDSLSLGLAGVWTQPGDRRPSGGEEPLALAGPARWALEWSLSAGIPLFARAQGL
                     WLEGKVFARPGLGDDAVGGALLLSYYAALLSPWVR"
     gene            complement(3959..5248)
                     /locus_tag="VFQ61_04585"
     CDS             complement(3959..5248)
                     /locus_tag="VFQ61_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9953750.1"
                     /translation="MMLRDPVHGLVSFESQEEAIIPALLEAREVQRLRRVRQMGLASL
                     AYPGADHTRFSHAIGAAHVMTRLLRRLRSIHEQLPFWQRVTTERARDALAAALLHDLG
                     HGPFSHLFEQILPDSHPHEDWTARIVLDPRTDVNRILCRYDSGLPTRVVELVRGRHEL
                     QYLARAVSGTFDVDRCDYLLRDAHFTGVGYGNYDLDWLLRSLCFDTTHQDPRGPSLAI
                     DGAKGIQAIESFLLARLFMFQQVYFHKTERACEWMASQIFRRVAQLSAEGTPVPGTPR
                     GIMSLTREGDADLDAYLSLDDAELWVALACYRQSSDPVLADLSQRLNARHLFKTHELF
                     GEQATPDARGRALEVAREIAAQHGLDPNVYVGLDAASTVAFDVKGDATRVVFPNGSAR
                     PLHEVSFLLGRLCGQRMERVRLIFAPELREEIQKALS"
     gene            6515..8695
                     /locus_tag="VFQ61_04590"
     CDS             6515..8695
                     /locus_tag="VFQ61_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953751.1"
                     /translation="MTNKPRHPPLSAIRLVDVLRKLPEKELDSIIARLKIRVDEAKRI
                     DVPSQVARALLLLPELRDPSLLPGPTRELLYRIAEARGVLVTPSLPAAVEPLILRGVV
                     FAKQGPRGIELLLPIAHMLVLKSWEGEDPRGVRALLHQVNVDVAGAIAANYLGRPATP
                     PVALSLEAAWEVLSDPSLLEREVAQLAPLERKLLSAIERVGGEVDTEELLDLEREPMR
                     LRGASGATPSRRGVGFALERRGFLIPVHPNRHVIPSEVAEVVGAQRRLEREAHRHEIR
                     NFVLAEDHAPHRARFAEDPAPLALALAIAVRDPSVEVKAGIGTPRSLVAKLATRFGRE
                     PDAVGFIAALSRAIGLWDPSAIGTAAPPGAFDVQTLGQALFEAWQRGGAWDEARADGE
                     VARAPAEAREPSAVGVLRELVLDGLRELGDGRWVPWEAIAGYVRSDARTPGVGRLIER
                     WAQRGGFEVMAPSEIARRIAFESLHWLGVVDIGDPEEDAADSIGPTLRLTPRGRAYVA
                     EQADARRAPEPSRFLDHQAVRVGGTARVGAVLALSPFTEIGGVESALDLWVTQATIAH
                     ALSAGFEADVIRARLEQLAPLPDPIARLLTQASAVVGRGEFVATMGFLWVEDPEIREL
                     LRTRRQTADLFLDPSPPAGLLVAPGADLDRVARRCRSLGVEVVVEGEVYRTRSIPPGR
                     GSGARRMDSSGTLSAMKLPRSSSSSSRPPAVRGTTPPPIKRGAE"
     gene            complement(8775..9281)
                     /locus_tag="VFQ61_04595"
     CDS             complement(8775..9281)
                     /locus_tag="VFQ61_04595"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9953752.1"
                     /translation="MLRVDEYLEQASSLSKEEFVSRRPGFFLLKRPKKNVSLQPGPPS
                     FGFATVTVKTDFDPFAGEWRIAPVVKRPDNPFPERISVGRATNCDIVLRLPFISKVHA
                     HILLEPGNKFSLRDNQPSNPTFLNHRKLEPGSTRSLSVGDMVGFGSLDFEFVDSARLY
                     DVLRRGLL"
     gene            complement(9527..11842)
                     /locus_tag="VFQ61_04600"
     CDS             complement(9527..11842)
                     /locus_tag="VFQ61_04600"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HET9953753.1"
                     /translation="MAGLALLACKGIACLGIACTGIAGNQAAGAASKSPPVSSSSSKL
                     PAATTGTRPATPAPSALDGGLWLPSQLAEQPDELRRYGFELDPQLLADPNSPLLASVV
                     HFSGCSASFVSDLGLFVTNHHCAVRALEYNSKPEDNLLKRGFLARRANEERSAGPSAR
                     LLITRSMRDVTQEVRAELAQAPDDVTRALALEAAQKRLVASCEAGQAARRCQLASFYS
                     GRQYFVIEREELRDLRIVYAPADGLGNFGGEVDNWRWPRHSADFAFFRAYTDAAGKPA
                     EFSPENVPYRPAHWLKLAATGVQTGDPIWVLGFPGRTARSKLRREVQETLEFTYPHRL
                     ALFEEYIQRIEQLGASDPELLIKATSLLRAFGNYRTKNRGEIEGMQRGKLLDRKQREE
                     QELLASLAAQPARATQLKKLLAEVDSAFDALRSTRRSDATLEFELPRVRLLAAATRIV
                     RLAEERQRPDAERDPEYQERNVSTLRDEIRSHLRSYYAPLDQALLGLAMQRLSRDAGP
                     AQPLLDVIVGKDHTPRAIEQAVQKLYASTRLADEKRALQLFDHATRAQLAQERDPLIA
                     LATRLRPLENAADLRRKQFTGRMLRLAPTYLELAMAAHPGRVAPDANATLRISVGHVG
                     GGQIAGGPDASGARLPHPTNPVFTWLSEIPRKHTGKPPFEAPRGLLDAIALAKVGEPR
                     LEPLTDRERKEVPVNFLSDASITNGSSGSPTLNSRGELVGLAFDGTYESVAADWLVDP
                     DSRSIHVDLRYLLFVLEQVEQAQELLHELGR"
BASE COUNT         2251 a         3684 c         3901 g         2209 t
ORIGIN      
        1 ctcgcgcgta tctttcgcgt atgtaccgcg ctcataagcg catctggctc accaattcct
       61 acttcattcc ggatcgaaac gtggtccgcg ccttggttcg ggcggcgcgg cgcggggtgg
      121 acgttcgggt cttgctgccg gacaagagtg atgtgcgaat cgcggattac gcgagccgcg
      181 cggtgtgggg caagctgctg cgcaatggtg tccgtatctt cacctggacg cgcagcgtac
      241 tgcactccaa aacggcggtc atcgatggca cgtggagcac gatcggcacg ttcaacctcg
      301 atcacctctc gctgcgcgtg aacttggagg tgaacgtgag cgtgctggat gagggcttcg
      361 cgagtgaggt agagggggtg ttcatcgccg acctgcagcg cgcccgcgaa gtggatccca
      421 agcagttccg ctttcgtccc ttggccgagc gttgcatcga gctgctcgca tatcggctac
      481 gcaagtggat gtgaacgagt agcctgcaaa gtcccgggaa cacgcaggtg ttcgcttcgc
      541 tatgctcccg ctagtggctg ctcacgagct cgaacgagct cgcgtcgggc gcgtatttcg
      601 gcagccggcg ctcgggtcgt tgacgcgttg gcggcttacc tgaacgacag ctgcagctca
      661 tcgagcacgc gcgcgaactc cggatccagc ggggcctcga ctacgatgtt cagggcgcgc
      721 aaggggtggg tgaaagccaa gcggtgggca tgcaacccaa gccgatggaa gccatactcg
      781 gcgcggtact tgcggttgat gtcaccgcgt ccgtaattta cgtcgccgac cagcgggtgg
      841 tgaatgtggc tgagatggcg gcgaatttga tggaaccggc cggtgaccgg aagtgcctcc
      901 accaatgagc aacgttctcg gggcgatgtc gccaaaagcc gaaaacgcgt caccgccggg
      961 acacgctctg ccccttcggc gcgcggcacc gggtgatcga tgatgcctga ctcgggcgga
     1021 cgcccccgca ccagagccca gtagcttttc tccaccgcac cgcactcgaa cagcttgccg
     1081 agggagctca ttgcggttgg gtgtcgggcg aagagtagcg cgccgctggt gccgcgatcc
     1141 agtcgatgcg cggggtacac ccgttgtcta aaatgatctc gaacgcggaa gagggccgta
     1201 tccgcgtcct gcgcaagccc gcggtggacg agcaggccag acggcttgtt cacgatgacg
     1261 aggtcctcat cctcgtacag tatttcgagc ggggcttgcg cgcgaggggc ggatggctca
     1321 ggtgtccgaa tggagctcat cgttcaaacg gcagtttttg gtgcttcggc accgagcgcg
     1381 aagcgaaacg aagttgaaca ccagcgcaga gaaccatcac gagaccattg ctttccctgc
     1441 catcaccttg agcgagttgg cgcgcaaata tgaggcgctt tggtccttgc gacaagctgg
     1501 gtccggcttc gacaaggctt cggtacgcgc gatttcggcg gagtttccag gctccttacg
     1561 cgagctagat gttctccccg acgagcaact catggcgcgg cgcgcccacg cgcgcgccgc
     1621 gtgccagggg gaagcgcccg aagcttggct gatttggctt ctcgcctatc attcggagat
     1681 gcgggtcgtg ctcgaggtac agcggcgaat tggcgagctc cggtcgtctc gcaaagcact
     1741 cggtatgagc gtgcaaccca aaccgatgat cgagcagctc atgttggacc cactcttcga
     1801 gcagggcttc gcctataccc cggagcagct gcatagcctg ttgtgtccac ccggaggccg
     1861 cctcaacaac gtggtattcg gttggctcgc ggagcgcttc ggcgttgacg ccgagcgtct
     1921 ggagcagacg ttgttcccgc gttcagaggc aggcgcaaac cacgaacgag gctagcacgt
     1981 tgacccaata cgaacttcgc acgtggtcgt cgataagaga tttgggcgcc gagactctct
     2041 ccgggctgca gcccaccgca ggaacgccgt tcctcagctt tgcgtggctg gatgctctcg
     2101 agcgcacggg ctgcgttaca gaagctcgcg gctggctgcc gatgcacatg acgctgcatc
     2161 gggcgggctc cgcacccgct tcgaagcgga caaactccgt cggggagcgt gtaggcggag
     2221 accacgcacg cggaaataac ggcgagctac tggcctttgc tcccgcatat gtgaaaacca
     2281 acagcgaggg cgagttcgtt ttcgatcacg gaatagcgcg cttcgctcaa acctcgttgt
     2341 ctctgccgta ctaccccaag ctgatcttgg cggtgccgtt cacgcccgtg accggctctc
     2401 ggctgctcgt tgcgccaggt caagacccgt ccgagatcac agccgtcttt gcgggcggag
     2461 tgcggtcctt ctgcgagcaa caagggttat ccggagccca tgttctcttt cctccagccg
     2521 ccgaggcctc cgcctgggtg acgagcggtt acgcgcggcg catcggtgtg cagtaccaat
     2581 ggcacaatca gggctacggg agcttcgatg agtttttggg caccttcaac gccaagcgtc
     2641 gtcaccagat tcggcgcgag cgccggcagg tcgcggagca ggatgtggaa atcgaagtgg
     2701 tgaccgggcg cgatttggag cccagttggg cagacttcat cttcgatttc tatcgctcca
     2761 cggtggacaa gtacttttgg ggaagacgct acttgaatcg cagcttcttc gaagaggtgc
     2821 ttacgacttt gcgcgaccaa gtacatctgg tggtcgcgcg agaccggggc tctcgtagac
     2881 gtctagcggg ggcgttcaac ttactgggca gcgacgcact gtacggccgc tattgggggg
     2941 ccatcgagga ccggaactgc ctgcacttcg aggtctgctt ctattctggg gtcgaggacg
     3001 ccattgcccg cggcctcaag cgcttcgagc cgggcgcggg cggggaacac aaactcgcgc
     3061 gaggcttcgt gccgacgcgc actttcagta cgcatcatta cgtagacccg cggctggaac
     3121 tcgcggttcg cgacttcctg gcgcgcgaag agtcggccat cgaagcagag ctcgagtcgg
     3181 tggagtaaat cgcgggtgct tcaccgaacc caaggcgaga gcagggcagc gtagtaactg
     3241 agcagcaagg cgccacctac ggcgtcgtcg cccaggccag ggcgggcgaa caccttgcct
     3301 tccagccata ggccttgtgc gcgcgcgaat aacggaatcc cggcactgag ggaccattcg
     3361 agcgcccaac gcgccgggcc cgcgagggcc aagggttctt cccctccgga tgggcgccta
     3421 tctcccggtt gggtccatac tcctgctaac ccaagtgaca gcgagtccaa agtgaggtcg
     3481 cacatggcgc aaccccactc taagtcattg gaccaacgga gaagaaacag cggccgcagc
     3541 tccagtccga tcgcggcggt gcggcgcacg tgagccgtgg catccattgc gtccgaatac
     3601 gccgcgaaca caccgacgct actgtagtag agcactcgac caacggcggt ggcgcgcgcg
     3661 acttccgaaa cttcagcgcc gacgccgaga gccaagttga cgtccccatc gaagcggccg
     3721 tagacgccgt cgccgtcgaa ccgctcgttg tccgcttcta tttcgccgcg aaccgaagat
     3781 gcgagccgcg gccgcgagtg ctgctcgcgc tccggttggt cggacgcgtt cgattggggt
     3841 ggcgcctgat cggccccacc gagcgactcc ggcgcgcctg gcgcggaact cgtaagtgcc
     3901 aacagagtta gtgtcgatct ggccgacacg cgcatcagac gagccaccgg ggtcaacttc
     3961 atgacagcgc tttctggatt tcctcgcgga gctcgggtgc gaaaatcagc cgcacgcgct
     4021 ccatgcgctg cccgcagagg cggccgagca agaacgagac ctcgtgaagg gggcgggcgc
     4081 tgccattcgg gaacacgacc cgtgtggcat ctcctttgac gtcgaacgcc accgtcgagg
     4141 cagcgtccaa cccgacatag acgttaggat cgaggccgtg ctgcgcggca atctcgcgcg
     4201 ctacctcgag cgcgcgtcct cgggcgtcgg gtgtggcctg ctctccaaaa agttcgtgcg
     4261 tcttgaagag gtgtcgagca ttgagccgtt gcgacaagtc cgcgagaacg ggatcgctgg
     4321 attggcgata gcaagccagc gctacccaca actccgcgtc gtccagagac aggtacgcgt
     4381 cgaggtcggc atcgccttcg cgggtcaagc tcataatccc ccgcggggtg ccggggacgg
     4441 gtgtgccttc tgcgctgagc tgggccacgc ggcgaaagat ctgcgacgcc atccactcac
     4501 acgctcgctc ggtcttgtga aagtagacct gctggaacat gaacaggcgc gccaacaaga
     4561 aggactcgat agcctgtatg cctttggcgc cgtcgatagc gaggctcggg ccacgcgggt
     4621 cttggtgagt cgtgtcgaag cacaagctgc gaagcagcca atcgagatca tagttgccat
     4681 aaccgacgcc ggtaaaatgc gcgtcgcgca gcagataatc gcagcgatcc acgtcgaacg
     4741 tcccgctgac cgcccgagcc aaatattgca gttcgtggcg ccctcgaacc agttccacaa
     4801 cgcgagttgg cagtccgcta tcataccgac aaagaatacg gttcacgtcg gtcctcgggt
     4861 ccagcacgat tcgagcagtc cagtcttcgt gcgggtggct gtccggcaaa atctgctcga
     4921 aaagatggga aaaaggccca tgccccagat cgtgcagcag cgctgcggcc agtgcgtcgc
     4981 gggctcgctc cgtggtcact cgttgccaaa aaggcagctg ctcatgaata ctgcgcagcc
     5041 gtcgcaacaa gcgggtcatt acgtgggcgg ccccgatcgc gtgtgaaaat cgcgtgtgat
     5101 ccgcgccggg gtaggctaag gaggccaggc ccatttggcg cacgcgccgc agtcgctgca
     5161 cctcccgggc ttcgagcaag gccgggatga ttgcctcttc ttgagactcg aatgagacca
     5221 ggccatgcac aggatcgcga agcatcatga gaaaactcta gaagtctaaa actcaaaatc
     5281 cctgtttgcg gaatctgaat acgcggatat cagaggcgcc tgaataccag gcgaatacca
     5341 gagccggcga ataccagagc cggcgaatac cagagccggc cgacaacaga gccggccaac
     5401 aacagagccg gcgaatgcca gagccggcca acaacagagc cggcgaatgc cagagccggc
     5461 caaacaacag agccggcgaa taccccggcc aacaacagag ccggccaaca acagagccgg
     5521 ctgaatcgaa gtgcggttcc aagtcgatcg gattggcaag gcctggcgag aactggccat
     5581 cccgcgatga gcacgccggg atcggcaaag cgtcgcggtc gcgacttgga tggttcggat
     5641 acgttcagcg gttggtgggg cacggatctc gttggcagcc gccagagcag gtgggggtgt
     5701 tgcgcccctg gcgggttacg agacatacgc gacgacagcc cctgaggcga gcggaatgcc
     5761 tagattctgg catagagcgt tactcgcccc agttcgaggc aacgtctcgt tccgtttggt
     5821 ctgggggcgc ggagctgccg cctcgtatcc ggggcaagtc aacttcacgc ctgactgatc
     5881 tacgcgccac ctggcgactc tcttctgggc tactgcacgc gagaagtcag ggtaccaatg
     5941 cggcgcatgc caccgctcgt ccggcgcagc caaccaagca aacctttgga tgagaccagc
     6001 ggcacgcagc ctcaaggaag ggcacatgca gccgcaagga agggcacgcg cagccgcaag
     6061 gaagggcgcg cgcagccgca aggaagggca cgcgcagccg caagaaagag ccgagcctcc
     6121 gcggaaccgt tcagaacttc gcagaaatgt ccgatagaac cgtgcgcgcg gcttcgcaaa
     6181 ctgtccggtg agtgtccggg gaactgatgg cggaactccc gtctaccccg atgcgggtga
     6241 cttcacctcg gatcgcgtcg actgagtgcc gaagaatcga cttcgttcct tgttcggggt
     6301 cacgcgtcgg caaggatatg ctggcaggaa tacggttcgc gacgatcatc taaccggaca
     6361 gcttgcgccg gatgagtttt cggggttgag tatcaaggtt actcctttgg cttaaagcgc
     6421 gctcaggtgt ccggacggtc cgcagggtgc atagagcgca gtttcttcag ggagcattca
     6481 agctttccgg gccaccggtt tcgtttcgat tcccatgacc aacaagcctc gccatccgcc
     6541 gctatccgcc atcagactcg tcgatgtctt gcggaaattg ccagaaaaag agctggattc
     6601 catcatcgcg cggctgaaga tccgggtcga cgaggccaag cggatcgacg tgccttcgca
     6661 ggtggcgcgc gcgctgctgc tcttaccgga gttgcgcgat cccagtttgc ttcccggccc
     6721 aacgcgagaa cttttgtatc gcattgccga ggcacgcggc gtgcttgtga ctccgagctt
     6781 acctgcggcg gttgaacctc tcatcttgcg gggcgttgtg ttcgccaaac aaggtccgcg
     6841 cggcatcgaa ctgctgcttc ccatcgcgca catgctcgtg ctgaagtctt gggaagggga
     6901 ggacccgcgg ggcgtccgtg cgctcttaca ccaagtgaac gtggacgttg cgggtgccat
     6961 tgcagcaaac tatctcggtc gtcctgcaac accgcccgtt gcgctatctc tcgaggcagc
     7021 ttgggaagtg ctttctgacc cgagtctact ggagcgtgaa gtcgcgcagc tcgcgccact
     7081 agagcgaaaa ctgctttcag caattgagcg cgtcggtggc gaggtagata cagaggagct
     7141 cctcgatttg gagcgtgagc ccatgcgctt gcgcggagcc tcgggggcca ccccttcgcg
     7201 tcgtggcgtc ggctttgcgc tcgagagacg tggattcttg attccggtgc accccaatcg
     7261 gcacgtcatc ccctccgagg tggcggaagt cgtcggtgcg cagcgccggt tggagcgaga
     7321 agctcatcgc cacgagattc gcaatttcgt gttggcggag gaccatgcgc cgcatcgcgc
     7381 tcggtttgcg gaagatccgg ctccgctcgc gctcgcgctg gcaatcgcgg tccgggatcc
     7441 cagcgtcgaa gtgaaagccg gcatcggaac gccgcgatcc ttggtcgcca aactggcaac
     7501 ccgcttcgga cgcgagccgg acgcggtcgg cttcatcgcg gcattgagtc gagccatcgg
     7561 tttgtgggac ccctctgcaa tcggcacggc cgcccctccc ggcgccttcg atgtgcagac
     7621 attgggacag gctctgttcg aagcttggca acgcggtggc gcatgggatg aagcgcgcgc
     7681 ggatggggaa gtggcgcgtg cgccagccga ggcgcgcgaa cccagtgcgg taggcgtgct
     7741 gcgcgagctc gtgttggacg ggctgcgtga actcggggat gggcgttggg tgccgtggga
     7801 agcgatcgcc gggtatgtgc gttcggacgc acgaacgccg ggagtagggc gcttgatcga
     7861 gcgctgggct cagcgcggcg gcttcgaagt catggcgcct tccgagatcg cccgtcgaat
     7921 cgcgttcgaa agcctgcatt ggttgggagt ggtggacatc ggcgaccccg aggaagacgc
     7981 ggcggactcg ataggcccca cgctgagact cacgccacgc ggtcgtgcct acgtagccga
     8041 acaagccgat gcgcgccgcg cgcccgagcc gagccgcttc ttggatcatc aggcggtgcg
     8101 agttggtggt accgcgcgag tgggtgcggt ccttgcgctc tcacctttca cggagattgg
     8161 tggagtcgag tctgctttgg acctctgggt cactcaggcg actatcgcgc atgctctgtc
     8221 cgcggggttc gaggcggacg tgattcgcgc acgcttggag caactcgcgc cgctgccaga
     8281 tcccatcgct cgtttgctga cgcaagcgag cgcggtcgtg gggcgcgggg agttcgtcgc
     8341 gaccatgggg tttctgtggg tcgaagaccc ggagatccga gagctgctac gcacgcggcg
     8401 ccaaacggcg gatctcttcc tggatccatc tccgccggcg gggctgctcg ttgccccggg
     8461 tgcagatttg gatcgtgtcg cacgacgatg tcgctcgctc ggggtcgagg tcgtcgtaga
     8521 gggagaggta taccgcaccc gttcgattcc tccgggacga ggaagcgggg cccgacgcat
     8581 ggactcgagt ggcactctgt ctgccatgaa gctgcctcgc tcgagcagta gcagctcgcg
     8641 gcctcccgcc gtgcgcggca cgacgcctcc gcccatcaaa cggggcgccg aatagccgaa
     8701 ctcccccggc gctctaccaa tgaagcggcg ccgaccggga gagccggata tcgttcggcg
     8761 acagttctgc gatcctacag caggcctctg cgcaggacgt catagaggcg cgccgagtcg
     8821 acgaactcga agtccaggga cccgaaaccg accatgtcgc ccacactcag gcttcgggtg
     8881 ctcccgggct cgagcttgcg atgattcaag aaggtcggat tcgacggctg attgtcgcgt
     8941 aaagaaaact tgttgccggg ctctagcaga atgtgcgcgt gaaccttcga aatgaaaggc
     9001 aaccgcagca cgatgtcgca gttggtggct ctgccgacgc tgatgcgctc cggaaacggg
     9061 ttgtccggac gtttgacgac aggtgcgatt ctccactcac cggcgaacgg atcgaagtcg
     9121 gttttgacgg tgaccgtcgc gaatccaaag cttggcggcc caggctgcaa agaaacgttc
     9181 tttttgggcc tcttcagtaa gaaaaaaccc ggacggcggc tcacgaactc ctctttggag
     9241 agggacgagg cctgttccaa gtattcgtcg acgcgcaaca tctacagccg ggggcgcctc
     9301 catcgttaac caacgtccgg gaggcacccc agataatagc cggaggcaag cccaccacgc
     9361 atgctcaagc gctgctcaac gtcgatattt gtgaaactca cattgggtta cccggcggcg
     9421 ctagggccca atgtgcgagt attcacgact gaggctctgc tcagtcgtgg cgagaaccgc
     9481 cctctaggct gcagtcctcc atcgcttcga atttccatgc tcgaactcac cgtccgagct
     9541 cgtgcagcag ctcttgggct tgctctacct gctcgagtac gaagagcagg taacgcaaat
     9601 ccacatgaat actgcggctg tccggatcta cgagccaatc tgcggcaacc gactcgtagg
     9661 taccatcgaa cgccaaccca accagctcac cacgcgagtt gagggtaggt gaacccgagc
     9721 tgccgttggt aatgctggca tccgacagga aattcaccgg gacctccttt cgttcacggt
     9781 ccgtaagcgg ttccagccgt ggctctccga ctttggctag agcaatggca tccagcagcc
     9841 cgcgcggggc ttcgaacggc ggtttgccgg tgtgcttgcg cgggatctcc gacaaccagg
     9901 tgaacacggg gttggtcgga tgaggtagac gcgcgccgga ggcgtcaggc ccgcccgcaa
     9961 tctgcccgcc gcccacgtgc ccgacgctaa ttcgaagcgt cgcgttggca tcgggcgcca
    10021 cgcgccccgg atgagccgcc atcgcgagtt cgagatacgt gggcgcgagc cgcagcatgc
    10081 ggcccgtaaa ctgtttgcgc cgcaggtcgg cggcattctc cagtggacgc agccgagtgg
    10141 cgagggcgat cagtgggtcg cgttcctggg ccaactgagc gcgagtggcg tgatcgaata
    10201 gctgcaatgc ccgcttctcg tcagccaagc gggtgctcgc atagagcttc tgcaccgcct
    10261 gttcgatggc tcgcggcgtg tgatccttcc cgacgatcac gtcgagcaag ggctgcgctg
    10321 ggcccgcgtc gcgcgacaaa cgttgcatgg ccaggcccag gagggcttgg tctagcggcg
    10381 cataatagct gcgcaagtgg gaccggattt catcccgcaa cgtgctcacg ttgcgctcct
    10441 gatactcggg gtcacgttcg gcgtcgggcc gttggcgttc ttcggcgagc ctcacgatgc
    10501 gggtcgccgc ggccagcaag cgcactcgtg gcagctcgaa ctcgagcgtt gcgtcgctcc
    10561 gacgtgtcga gcgtagcgcg tcgaatgcgc tgtcgacctc cgccaagagc tttttcagct
    10621 gggtcgctcg agccggttga gccgcgaggc tcgccagcaa ctcctgctct tctcgttgct
    10681 tgcgatcgag cagcttgccc cgttgcatcc cctcgatctc gccgcggttc ttggtgcggt
    10741 agttcccgaa agcacgcaac aagctggtgg ccttgatcag aagttccgga tcactcgccc
    10801 ccagctgttc gatgcgttga atgtattcct cgaacaacgc gagacggtgc gggtacgtaa
    10861 actccagcgt ctcctgtact tctcgccgca atttcgagcg ggccgttcgc cccgggaaac
    10921 ccaagaccca aatcggatca cccgtctgga ccccggtggc ggccaacttg agccaatgcg
    10981 cgggccgata cgggacgttt tcgggcgaga actcggcggg cttgcctgcg gcgtccgtat
    11041 acgcccggaa gaatgcaaaa tcggcggagt ggcggggcca ccgccagttg tccacctcgc
    11101 caccgaagtt gccgaggcca tcggcaggtg cgtagacgat gcgcaaatcg cgcagttcct
    11161 cgcgttcgat cacgaagtat tgacgtccgg aatagaagct cgcgagctgg caacggcgcg
    11221 cggcttggcc ggcctcgcag ctcgcgacca gacgtttctg ggccgcttcc aaggccaggg
    11281 ctcgcgtgac atcgtcgggc gcttgggcga gctcggctcg aacttcctgc gtgacgtcgc
    11341 gcatggagcg cgtgatgagg agccgcgcgc tcggtccggc tgaacgctcc tcgttcgctc
    11401 gacgagccag gaagcctcgt ttgagtaagt tgtcttccgg cttggaattg tattccaagg
    11461 ctcgcaccgc gcagtggtgg ttggtgacga acaagcccaa atcggagacg aaagacgccg
    11521 aacaaccaga gaagtggacg accgaagcca atagcggtga gttgggatcg gccagcagct
    11581 gcgggtccaa ctcgaatcca tatcgtcgca gctcgtcggg ctgttcggcg agctggctcg
    11641 gcaaccaaag gcctccatcg agcgcgcttg gcgcgggcgt tgcgggtctg gttccagtcg
    11701 tcgcagcagg cagcttgctg ctagaactcg agactggcgg agacttcgaa gcagcaccag
    11761 ccgcctgatt gccggcaatg ccggtgcagg cgatgccgag acaagcaatg cccttgcagg
    11821 caaggagcgc caggccagcc atcggaaaag cggagcgtag cgggagcatg tcggccaggg
    11881 ttaagggtta accgcgcctc gcgccagagc gattcgcgcc gtccgcctcg gccggcttca
    11941 acgcgcgctt tcgcggcagc tctcgatgcc tcggcgtccg gactgtctgc gggctcgggg
    12001 tgctctgacc ggctcactcg aggtccacgc ggtgctccgc tcgtt
//
LOCUS       DASTKU010000129         6352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1585882, whole genome shotgun sequence.
ACCESSION   DASTKU010000129 DASTKU010000000
VERSION     DASTKU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585882"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..905)
                     /locus_tag="VFQ61_04605"
     CDS             complement(198..905)
                     /locus_tag="VFQ61_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET9953754.1"
                     /translation="MGTVDLLSHTTALSGVPRDRLEALARAAVQQTYPRGAILWRAGD
                     VPQNFVVVQRGLVKLVRHANRARAICGLFGPTESLGDLSAIRGLPYPADAIAASESVS
                     VVCLPRQLVMQVFADDPKLFMSLACAIEEKVEALLDKIDVLSAGSVEARLATLLLKLN
                     ARFGDDFDDGTSEIPVALSRRDLAELVATSFETAIRVMSRWEREGVLSTTRRGFTLRD
                     VSKLATVSGTVTALAAE"
     gene            complement(1074..1718)
                     /locus_tag="VFQ61_04610"
     CDS             complement(1074..1718)
                     /locus_tag="VFQ61_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9953755.1"
                     /translation="MPGPVRRIAVLGDSLSDPSVGGGAYLAPVREACGSHVEVRNFAK
                     GGFMVNQMRRRFERELLPHLREYSDLIVFGGVNDLYSDETAGRSLTKIQADLAYIYKA
                     AKVHNVRVIALTVAPWGGFSRYFNERRALTTERLNAWIRAQAEQGLVDRAIDTYALLA
                     CNGTPVLCPEDAKPFKDGLHFGRAGHRKVGAALLSAAFSECLPPAPSDGSNGSR"
     gene            complement(1887..3743)
                     /locus_tag="VFQ61_04615"
     CDS             complement(1887..3743)
                     /locus_tag="VFQ61_04615"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9953756.1"
                     /translation="MRTDSLRLPALPDAIGRYEVVSLLGEGGMARVYLAMQRGPFAAT
                     KLVAIKHLRAEVSHDDQFLNMFVDEARIALRLNHPNVVHTYEVVAEENEYYLLMEFLE
                     GQTLMNLLRYAGRESIPLSDQLWVLTQVLAGLQYAHELRDFDGTRLNIVHRDVSPSNV
                     FVTYAGEIKLLDFGIAKAAGAISATQQGIMKGKLGYAAPEQCLGRQVDNRTDVFAVGV
                     MLWEAMAKKRRWSGETQQAMMQARITHTEPAIEEVWPEVPMPLAAISRRALAQDPEER
                     YASAAEFQADLDQYLRSMPRPPGPENIAKLMAEVFQEEREELRRIIEERIGSTRYTLP
                     TGSQAAPASLPPRSIGARALHGNPAESRRASSRPGPATDGSGERDEKSGLSEANQNSL
                     SGLKTGWLASIAAADATPKRRALFIGLSCGGVLALLAAIWVGMRGESPQVPAGTVTPL
                     ATLPLASASSSLTGGSPMPNATVAGAGVSAPLVAPVQLIKARISVTPPWATVHLDGKR
                     LEGLPTETVLPRDGKDHILTAGANGYESLERTLSFDRDFSLEVALRPAQAFRGGFVKK
                     KKEEPVTSFAVPAAAAPAPSAGRGDRSEPGQDLRPTVGNRSLRGIDESDPYR"
     gene            4139..4846
                     /locus_tag="VFQ61_04620"
     CDS             4139..4846
                     /locus_tag="VFQ61_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9953757.1"
                     /translation="MKAAEVYRVTRKASDLRFNLYICRPIAAVLVSYVQSTRVTPNQI
                     TFLSFAIAGVSCLGLLLLPGYWGLIASVAALELAYVFDCADGMLARLRGKASSAGHLL
                     DFLMDELKAFLLLGAAATRLFIESGRVVFLFWGVFGLVALASGIALTTFLRRPEVVRD
                     GPSGESSRSSPSLLQRAVAAIESVARFLIHYPSYIWIVAGFGRLEMFLYPYVAVNAAY
                     AARALLGVAVRFGRFED"
     gene            complement(4805..>6352)
                     /locus_tag="VFQ61_04625"
     CDS             complement(4805..>6352)
                     /locus_tag="VFQ61_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953758.1"
                     /translation="SPLRNAASVLFVGASVVATTLGFLETCWLTPRAYAPTLAFGWPL
                     AGLFMGWVLVRRARAPHAAAAGAWCCLAICSAASGYVWIWAFESASALAGCRWLFPLA
                     LGALFGAALTLNVRAFLSPLREHGVLASALAPGPLASSFCGWVAALCLFPGLGILRRG
                     VFIAALFSALALFAERLSNSIQEGPRRRNFCVRWLLVAAPVISAAALLFAEWRVPLQL
                     LKGHSPEVLYTLRTPYREHVFVRLQGGLLMFSDGVLGFSSSDAARFAEALVHPALALS
                     SRHDSVLAIVDGTGLVETEILRWPSVRHLARLSFDPTLSNFSERAAAFAAWRGAARER
                     ARVEAIDREPSQFILSSQRRFDVIIADFGDPTNYQAGKYYTLGFLNILRERLNPGGTL
                     ALQITNRHRTPQTQASILATLRAAGFESRIYSAPLPVLGEWGFVLCSAPGPRDAKIPH
                     DFPRGTSFVTASTLGSLFATPRELVVTDSASNAPINMLFSQPAVELYLEEEASILESP
                     EAHRHPE"
BASE COUNT         1208 a         2005 c         2015 g         1124 t
ORIGIN      
        1 ttgcgggagg acggatgcga gtcagcctat gttttcgctg aatcaggccg gtcgaaccgc
       61 tgttatgagc tcaaacagcg gttcggccgg tccgcccgcc tacaccttgg caccggcaat
      121 tcataaccgg atttacttca aacaccccat tccatacgaa ccattctgcg agctcacgcc
      181 tatcggcggg agcgccttca ttcagctgcg agggccgtga ccgtaccgga tacggtcgcg
      241 agcttactca catcccgcaa cgtgaagccg cggcgggtcg tggagagtac gccttcacgc
      301 tcccaacgag acatcacgcg aatcgcggtc tcgaacgagg tggctaccag ctctgccaag
      361 tcgcgccgcg agagcgcaac cgggatctca ctcgtgccgt catcgaagtc atcgccgaag
      421 cgagcattga gcttcaacaa cagagtggcg agacgcgctt cgacgctgcc cgcgctcaat
      481 acatcgatct tgtcgagcag ggcctcgacc ttttcttcta tcgcgcaggc gagcgacatg
      541 aagagtttcg gatcgtcagc aaagacttgc atgacgagtt gtcggggcag acagaccacc
      601 gataccgatt cactggcggc gatagcgtct gctgggtagg gaagtccgcg aatggccgag
      661 agatcaccca agctctcggt tggcccaaac aagccgcaaa tggcgcgtgc ccggttggca
      721 tgccgaacca acttcacgag tccgcgttgg actacgacga agttttgcgg cacgtcgccg
      781 gctcgccaaa gaatggcgcc tcgtggatag gtttgctgaa cggcagcgcg cgcgagggcc
      841 tccaagcgat cacgcggaac tcccgacagt gcagtcgtgt gtgagagaag gtcgacagtg
      901 cccatagcga tcgcccgtaa gagcaagttg cgcgccgacc tccaattaag cggcgaatga
      961 cttagatcaa gcccgctatg cccgatggac tcgggaactt gcgcgcgatt ttcgcgcagc
     1021 cccgcatgac acgcgcaagt ggtgcggatt gccgcaatcc tgcaagggag cgctcacctc
     1081 gagccattgg agccatcgct cggtgcaggt ggcaaacact cgctgaaggc ggcgctcagc
     1141 agtgcggcgc cgacctttcg gtggcccgcg cgcccgaaat gcaggccatc tttgaagggt
     1201 ttcgcatctt cggggcacag cactggcgtg ccattgcagg cgagcagagc gtacgtatcg
     1261 atcgctcgat ctacgagccc ttgctccgcc tgagcgcgga tccaggcatt cagccgctcg
     1321 gtggttaggg ctctccgctc gttgaaatag cggctgaaac caccccaggg agctacagtc
     1381 aaggcgatga cgcgcacatt gtgcaccttt gccgccttgt agatataggc caggtccgcc
     1441 tggatcttgg taaggctacg ccccgctgtc tcgtcgctat acaggtcgtt gactccgccg
     1501 aagacgatca ggtccgaata ctcccggagg tgtgggagca gttcccgttc gaagcggcga
     1561 cgcatctggt tgaccatgaa gccgcccttc gcgaagttgc gcacctcgac gtgcgaaccg
     1621 cacgcctcgc gtaccggcgc gaggtaggcg ccgcccccga cgcttggatc actcagcgaa
     1681 tctccgagga ccgcgatgcg ccggaccggg cccggcacag tccgaggggg cgccccgccg
     1741 gctgcacacg gggccgagtt cggctcggcc cgagctgagc cacccaacca aagcaacaga
     1801 cccaagagcc cggcccggac gacgctcctc ctgtttctca ccctcgcgac gcagcgcatg
     1861 atcggataac cccccgggat gaccggctaa cggtaagggt cggactcgtc gatgccgcgt
     1921 aggctgcgat tgccgaccgt cggcctcaga tcctgtcccg gctcgctacg gtctcctcgc
     1981 cccgcactgg gcgctggcgc tgctgcggcc gggacggcaa agctcgtcac cggctcttcc
     2041 tttttcttct tcacgaaccc gccacggaac gcctgcgcgg ggcggagcgc aacctcgagg
     2101 ctgaagtcgc ggtcgaatga cagcgtgcgc tcgagagact cgtagccgtt cgcgcctgcc
     2161 gtcaggatgt gatccttacc gtctcgaggc aggacagttt ccgtaggcag tccctcgaga
     2221 cgcttgccgt ccaagtgcac ggtggcccag gggggtgtga ccgagatccg cgctttgatg
     2281 agctgtaccg gagcaacgag tggagccgaa acccccgcgc cagccacggt tgcgttcggc
     2341 atgggtgagc cgccggtgag cgagctcgag gccgatgcga gcggtaacgt agcgagaggt
     2401 gtcacggtgc cggcgggtac ttgcggactc tcaccgcgca tccccaccca aatcgctgcg
     2461 agcagcgcca gcacgccccc gcagctcagc ccaatgaaca aggcccgtct tttgggcgtc
     2521 gcgtcggctg cggcaatgga cgccaaccat ccggtcttca gtcccgaaag gctgttctgg
     2581 ttggcttcgg acaatccaga tttctcgtcg cgctcgccgg aaccatcggt tgccggcccg
     2641 gggcgactgc tggctcggcg cgactcggcc gggtttccgt ggagggcacg agcgccaatg
     2701 ctgcgggggg gtagagacgc tggagccgcc tgggacccag tgggcagcgt gtagcgtgtg
     2761 cttccaattc gctcttcgat gatgcgacga agctcctcgc gctcttcctg gaacacctcc
     2821 gccatcaact tcgcgatgtt ctccggtccg gggggacgcg gcatgctgcg taagtactga
     2881 tcgaggtccg cctgaaattc agcggccgac gcatagcgct cttccggatc ctgcgcgagc
     2941 gcgcgacggg agatcgcggc gagcggcatg ggaacctcgg gccaaacctc ttcgatggcg
     3001 ggctcggtgt gcgtaatgcg tgcctgcatc atcgcttgtt gggtctcgcc gctccaacgt
     3061 cgcttcttcg ccatggcttc ccacagcatc actccgaccg cgaacacgtc cgtgcgattg
     3121 tcgacttgcc gcccgaggca ctgctcgggg gccgcgtagc cgagcttgcc tttcatgatg
     3181 ccctgctgag tcgccgaaat cgcgccggca gccttggcga tgccgaaatc gagcaacttg
     3241 atctcacccg cgtatgtcac gaagacgttc gaagggctca cgtcccgatg gacgatgttc
     3301 aagcgcgtac cgtcgaaatc gcggagctcg tgcgcgtact gaaggccggc gagcacctga
     3361 gtcagcaccc acagctgatc cgacagcggg atactctcgc gtccggcgta acgcaggaga
     3421 ttcatcaagg tctgcccctc gagaaactcc atcagcaaat aatactcgtt ctcttcggcg
     3481 acgacctcgt aggtgtgcac cacgtttggg tgattgaggc gcagcgcgat gcgcgcctcg
     3541 tccacgaaca tgttgagaaa ctgatcgtcg tgcgagactt cggcccgcag gtgcttgatc
     3601 gcgacgagct tggtggcggc gaacgggccg cgttgcatgg ccaggtagac ccttgccatg
     3661 cccccttcac cgagcagtga aaccacttca tagcgcccaa tcgcgtcggg aagggcaggc
     3721 agtcttaagc tatctgtgcg cacgggttac cccccataga ttaatcatgg ttgctctccg
     3781 tctcaaagag cacgcccggg atgagccgcg aaacgcggcc gagaagacgc cgagccaggc
     3841 gcggggctgg gacgctgtgg gatgcagcca ataccccgga tatagcagcc acgtccgcca
     3901 gagcactgaa ttgaccggca tcccgtagtt gcccgctcca agtcccgggg cgagaccgga
     3961 gtcggcgccc gtccccccgc acgggggaca ggcacgccgg agggccgcga gcacttccac
     4021 gcgggccacg gtacttcgaa cggcaccaag atcaagggag gcacgaagca accgaatggc
     4081 agcaccactg gggggcgtcg taaccccggt cccgggattg gtgtaacctg cgcgggccgt
     4141 gaaagctgcc gaagtgtatc gcgtcacccg taaggcttcc gacctgcggt tcaatctcta
     4201 tatttgtcga ccaatcgcgg cggtgctcgt gagctacgtg cagagcaccc gcgtcacccc
     4261 aaaccagatt acttttttgt cgttcgcgat cgcgggcgtt tcgtgtttgg ggcttttact
     4321 gttacccgga tactggggtt tgatcgcgag cgtggcggcg ctcgaattgg cttatgtttt
     4381 cgattgcgcg gatgggatgc tagcgcggct ccgagggaag gcatccagcg ccgggcatct
     4441 cttggacttc ttgatggatg agctgaaggc ctttctgctg ctcggcgcag cggcaactcg
     4501 gctcttcatt gaatcggggc gagtagtctt tctattctgg ggggtcttcg ggctggtggc
     4561 tttggcctcc ggtatagcgc tcacgacgtt tttgcggcga ccggaggtgg ttcgggatgg
     4621 gccgtctggc gagagttccc gctcctcacc gagcctgctg caacgagcgg tcgcggcgat
     4681 cgagtcggtc gcgcgctttc tcatccatta cccgtcgtac atctggatcg tggccgggtt
     4741 tggtcgactg gaaatgttct tgtacccgta cgtcgcggtg aacgcggctt acgcggcgcg
     4801 cgcgctactc ggggtggcgg tgcgcttcgg gcgattcgag gattgaagcc tcctcttcca
     4861 ggtagagctc gacggctggc tgcgagaaca acatgttgat tggggcattc gaggcggagt
     4921 ccgtgacaac gagctctcgc ggtgtggcaa acaaactgcc caatgtggaa gcagtaacga
     4981 agctcgtgcc gcgcggaaaa tcgtgcggga ttttggcgtc gcgcggaccg ggcgctgaac
     5041 acagcacgaa gccccactcg cccaataccg gcaggggtgc actatagatc cgagactcga
     5101 agccggccgc gcgcaaggta gccaagatcg atgcttgagt ctgcggtgta cggtgccggt
     5161 tcgtgatctg gagtgccagc gtgccgccgg ggttgagcct ctctcgcaga atgttcaaaa
     5221 agcccagcgt atagtatttg ccagcttggt agttcgtcgg atctccaaag tctgcgatga
     5281 tgacgtcgaa gcggcgctgg ctcgaaagta tgaattggct gggctcccgg tcgatggctt
     5341 cgacgcgcgc ccgctcgcga gcggcgccgc gccacgccgc aaacgccgcg gcacgttcgg
     5401 agaagttgct gagggtcgga tcgaaactga gccgcgcgag gtggcgcacg cttggccaac
     5461 gcaggatctc ggtttccacc agccccgtgc cgtctacaat ggccaaaacc gagtcgtggc
     5521 gcgagctcag cgccagggcg gggtggacta gtgcctcggc gaaccgcgcc gcatccgagc
     5581 tcgagaaccc caaaactcca tccgaaaaca tgagaagccc gccttgcaaa cgcacgaaca
     5641 cgtgttcgcg ataaggcgtc cggagcgtgt ataggacctc tggtgaatga cccttgagca
     5701 gctgcagggg cacgcgccac tcggcgaaca gcagtgcagc ggcggagatc accggcgcgg
     5761 ccaccaacag ccagcggaca cagaagtttc tgcggcgtgg cccttcctgg attgagttcg
     5821 aaaggcgctc ggcaaaaagg gccagagccg agaacagcgc ggcaatgaac actccgcgtc
     5881 gcagaattcc tagccccgga aacaagcaca gggcggcgac ccagccgcag aacgagctgg
     5941 ccaatggtcc aggcgccagc gcgctcgcca ataccccatg ttcacgcagc ggtgacagaa
     6001 acgctcgcac attgagcgtt aacgcggctc cgaacagcgc cccgagcgcg agcggaaaca
     6061 gccagcggca cccagcgagc gcgctcgccg actcgaacgc ccaaatccaa acgtaccctg
     6121 aagcggcgct gcagatggcc aggcaacacc aggcgcctgc cgcggcggcg tgcggcgctc
     6181 gtgccctgcg caccagcacc caccccatga aaagcccagc cagaggccag ccaaacgcaa
     6241 gcgttggggc atacgctcga ggggtaagcc agcaggtttc gaggaacccg agcgtcgtgg
     6301 caactacgga cgcgccaacg aacagcacgc tcgcggcatt cctcaggggc ga
//
LOCUS       DASTKU010000130         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_25074,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000130 DASTKU010000000
VERSION     DASTKU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25074"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFQ61_04630"
     CDS             <1..583
                     /locus_tag="VFQ61_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9953759.1"
                     /translation="MELVFLPGFSTKDSIDELSGRGVGMDVIKTTIEGLRGRVQLRSS
                     AGRGSRVLMTVPLTLAFIEAMVVRERDRLLALPIEKVFEVFKIGAEQRAFHSADGQTM
                     LRVRDRFVPVCWLHRYFEEPGGDETLDDRVVVVVQTSQGNLALPVDRLLGNQQIMLKP
                     LRGLLSQVRGAAGCGMLRSGDVALAIDCERLHA"
     gene            627..1412
                     /locus_tag="VFQ61_04635"
     CDS             627..1412
                     /locus_tag="VFQ61_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET9953760.1"
                     /translation="MPPSSLDVLRSWVRTHFGITFTQDRIALFRSQVETLCRDIGISV
                     DTMLLKVSCGDREVTQRLAEAVSTNYTYFFREDSAFTLLRNTVLPKIQGPVRIWSAAA
                     SSGDEAYSIAITCAESLGTAAFERVRVLGTDISARQLRIAEDGVYSLQHLDSLNPERR
                     KLWFTPAGLGQFKLQPELRKMCLFRRMNLTAMPWPFTQRFHAIFLRNVLYYFEPEQGR
                     AILEACYDACEPGGWLFTSVTEPLIAQNTRWSSVGVGVYARRG"
     gene            1412..2470
                     /locus_tag="VFQ61_04640"
     CDS             1412..2470
                     /locus_tag="VFQ61_04640"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HET9953761.1"
                     /translation="MVEPKRKVRVLVVDDSALVRSILTRGLSADPEIEVVGSARDPYE
                     ARDQLVKLKPDVMTLDVEMPRMDGVSFLEKFMSVMPTPTVVLSSLTTRGSVLALQAME
                     AGAVEVMTKPSAAVAEGLEVMMANVVRVVKAAARSHVSQRRATGIEKPQETSNALDNT
                     TDRVIALGASTGGVAALGRILPVFPAWTPGIVIVQHMPPGFTADFAQRLNDKCQMRVS
                     EATDGQRVLRGHILVAPGGTRHCEVRRMGGEYRIRLVEGQPVSGHSPSVDVLFESLAR
                     SAGRNASACLLTGMGHDGAKGLLSLRHAGGRTFAQDRETSAVWGMPAAACDIGAAERA
                     LPLESIPNVLVSMATANQ"
BASE COUNT          420 a          733 c          844 g          483 t
ORIGIN      
        1 catggaattg gtgtttctac ccgggttctc gaccaaagac tcgattgacg agctctctgg
       61 cagaggggtc ggaatggacg tcatcaagac caccatcgag gggctgcgcg ggcgcgtgca
      121 gctgcgcagt agcgccgggc gtggttcgcg cgtgctgatg accgtgccgc tcacgcttgc
      181 cttcatcgaa gccatggtgg tgcgcgagcg cgaccgtctg ctggctttac cgatcgaaaa
      241 ggttttcgag gtcttcaaga tcggcgccga gcaacgcgcg ttccactctg cggacggcca
      301 gaccatgctt cgggtgcgcg atcgcttcgt gcctgtgtgc tggctgcatc gctacttcga
      361 agagccgggc ggcgacgaga ccctcgacga ccgagtggtc gtggtggtgc aaacctcaca
      421 gggcaacttg gccctgccgg tcgatagatt gttggggaac caacaaatca tgctgaagcc
      481 gctgcgcggc ctgttgtccc aggtgcgggg ggccgccggt tgcggcatgt tgcgctccgg
      541 ggacgtggcc ctcgcgatcg attgcgagcg cctgcatgca tgagtggggt tcgcgcgcgt
      601 cactgccgcc gggacctcgc ggcagcatgc ccccctctag cttggacgtg ctccgcagtt
      661 gggttcgcac ccacttcgga atcaccttca cgcaagatcg catagcgctc ttccgctcgc
      721 aggtggagac gctctgccgc gatatcggca ttagcgtgga caccatgctg ctgaaggtga
      781 gctgcggtga tcgcgaagtc acgcagcgcc tggccgaggc cgtctcgacc aactacacgt
      841 atttctttcg agaagacagc gcgtttaccc tgctacgcaa cacggtattg ccgaagatcc
      901 aggggccggt gcggatttgg tcggctgcgg cctcgagcgg agacgaggcg tacagcatcg
      961 cgattacttg tgcggagagt ctcggcaccg cggccttcga gcgggtgcgc gtgctcggca
     1021 cggacatcag cgcgcgtcag ttgcgcatcg ccgaggatgg cgtttactcg ttgcagcatc
     1081 tggactctct gaatccggag cggcgcaaac tgtggtttac cccggccggg ctcgggcagt
     1141 tcaagctcca gccggagctg cgcaagatgt gcctgtttcg gcgcatgaat ttgaccgcta
     1201 tgccttggcc tttcactcag cgtttccacg ccatcttctt gcgcaacgtt ctctattact
     1261 tcgagcccga gcaggggcgc gccatcttgg aggcttgcta tgacgcatgc gagccgggtg
     1321 gctggctatt caccagcgtc acagagccgt tgatcgcgca gaacacacgc tggagcagcg
     1381 tgggagttgg agtttacgcg aggagaggct gatggtggaa ccgaaacgca aagtgcgagt
     1441 cttggtggtg gacgattcgg cgctcgttcg ttccattctg acgcggggct tatcggctga
     1501 tcccgaaatc gaggtcgtgg gcagcgcccg cgacccttac gaggcgcgcg accagctcgt
     1561 gaagttgaag cccgacgtca tgacgctcga cgtggagatg ccacgcatgg acggcgtgag
     1621 cttcctcgag aagttcatga gcgtgatgcc gacgcccacc gtagtcttga gctcgctgac
     1681 cacgcgcggc tcggtgttgg cactgcaggc catggaggcg ggggccgtcg aggtgatgac
     1741 gaagccctcc gcggcggtgg ccgaagggct cgaagtcatg atggcaaatg tggtgagagt
     1801 ggtgaaggcc gccgctcgta gccacgtgag ccaacggcga gcgacaggca ttgaaaagcc
     1861 gcaggagacg tccaacgcgc tcgataacac gacggaccga gtcatcgcgc tcggtgcgtc
     1921 gacgggcggc gtggccgcgc taggccgcat cttacccgtt tttccggcgt ggacgcccgg
     1981 catcgtaatc gtgcagcata tgccaccagg cttcacggct gactttgctc agcggctgaa
     2041 cgataagtgc cagatgcgcg tgtccgaagc gacggatggt cagcgcgtgt tgcgagggca
     2101 catcctggtt gctccgggcg gtacgcgcca ttgcgaggtg cgccgcatgg gcggcgaata
     2161 tcgaattcgg ttggtcgaag ggcaacctgt ctccgggcat tcgccttcgg tggatgtgct
     2221 gttcgagtcg ctcgcccgtt cggcagggcg caacgcgagc gcctgcttgc tcaccggtat
     2281 ggggcacgac ggggccaaag gtttgctctc gctgcgtcac gccggtggtc gcacgtttgc
     2341 gcaagatcgc gagacgtcag ccgtgtgggg gatgcccgcg gcggcgtgcg acatcggcgc
     2401 ggccgaacgc gctttgccgc tcgagtccat tcccaacgtg ctcgtctcga tggcgacggc
     2461 aaaccagtag tacgcgcgag
//
LOCUS       DASTKU010000131        10506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_693658, whole genome shotgun sequence.
ACCESSION   DASTKU010000131 DASTKU010000000
VERSION     DASTKU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10506
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693658"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFQ61_04645"
     CDS             complement(<1..279)
                     /locus_tag="VFQ61_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953762.1"
                     /translation="MSRQRSGSNFRRRMFGVLQQCFGFGICGWLLACAPQSSSEPSSN
                     EPPTSGPPTPTASSGGWKPASTCPTLEEQNFSFFLMSHAAVVRQSGSPD"
     gene            549..3983
                     /locus_tag="VFQ61_04650"
     CDS             549..3983
                     /locus_tag="VFQ61_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953763.1"
                     /translation="MVKPNQPEAQEIHRELARYINLKLAALGEPTSSATADAYFMELA
                     GPLLRNHFQKDALLGWPLCPADSRIQRFLDEYLSEVCPEGAPRIPEKTFALDRPGLAR
                     VLSLPVSADRFASPFLTSYRVQQGVLHNPKSDRRTTQGVFHVTEGGLPIPADKLAVPK
                     ITFARLLAAALRPPEAVLTLPFTADQPQPAQLFVSLLLRPLVIPETENEPAKSMEIRF
                     FAPGSLVSNLDFVEGIFGNGGDAFLPENDAALDVKSFTGHSGCVIVAPHIVGLPKKAL
                     GLPHYDQATERQRRDGMCYRDENEPYNGGNAFKITCRDERGVMVTVIADNYYGYCKKE
                     VKTQISYAANLYGACEEEHAGGALAFPTYVIGQDFYADRSFVLKNTSFERAMEILGSR
                     VEVKPNRYAVDRKYPDVFYVPEDSEFHVADGQVRWKYQGAVQTLTLRTGDIYVLPSGY
                     RLRLEKQLGASLIRLVACRPDGVLCHKPCTVSGGGKSEISKSLKPMIQPAPIFVKEYQ
                     RDMDVVAEILKRDFTKIYKDRAPDERARRPLLGLDRSLGSVIQLLTPSPEYTDEYNAW
                     VRALPQTIRQLVFVLKRYYRPEWGDDWRSHFTVDRINGYPGHELKFNNQRLVGSYLRV
                     GFEPGSNSWRMYKLRPDFNPADKVQVEDDITASVVVPRSRVSSFPAAEKNQSVKIVQN
                     CEALLFQRPDDAIHRGFDHQAEADLATPGTFITNFEPLPRAQVQALADKAADFDRFTA
                     PMKELLEGFLKAPQAEYAVSSAHPRIVDGKPSKNPRYLQRRPDLVQHREAYVAEIGQR
                     LHEEIPEQKPVPAAVQAVLAGRRASPPQPEIGLPPLAVYSPIHYQELPELFMDFLSSL
                     TGKSPSTTGFGSEGALTKGPFNALWPVVDMNNALLSFILTGYSGFTTAAGFIGPNYKV
                     DHDISMLVPEIWCRMRTEERDARYLIDHGYLEKVNDFDFESRRVLASRLGYRITPRFV
                     DHFLGRLFETPNTVFTEEMLRPEKQSLEMFAGGVDAIVETQTRVARSYFEDGSVDAAC
                     PPLKALLHIMVHGSFDGMTLEHPEVRKLFTREATLESAWYKERLRTKQQRDLALYRRH
                     AASLEEVSPVVRGSLAGFDARLSYVQERLQQVASPRYVDELVGTLGADPFHLQLAR"
     gene            complement(4133..5065)
                     /locus_tag="VFQ61_04655"
     CDS             complement(4133..5065)
                     /locus_tag="VFQ61_04655"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9953764.1"
                     /translation="MGRIRRVEGTLECFLEPEHLVGRGPQCALWLQAKYVSGQHCVIR
                     WTGYLWQILDRGSRNGTRVDGQRIERGYAHDLHEGSVICFGQAEQWILIDASPPVTML
                     VCEQTRVAIPENGGRIELSTSSGVRHEVRQSADGRWWLYGSSSEPVRTEPQRRQLVEP
                     NAVLELEGQRYRFVCPQLVNATCAHEEAYRDPVIEFDVSHEREFVQISLHNAERSYSL
                     GSRSFNLLLLQLAKERLADQAAGRPAGECGWIYKSDLADALAYSPQQVDGDVHRLKRH
                     MEKHALTEAGYLIERRPGTTQLRLALSEIKIHES"
     gene            5156..6001
                     /locus_tag="VFQ61_04660"
     CDS             5156..6001
                     /locus_tag="VFQ61_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9953765.1"
                     /translation="MQTNQGWSEASRRNIEGIAMNSDLDAEQTSSGNAWQPWGQVLEN
                     QLQIYGRGEPSEIEEDRTLLATRVLELELIWPLLKAGLIKVSRTLFTSTHTSFLLAPG
                     AAARAKKSLPERYAEILERTLLDSGPKVVAAELGYSISGIGTILRQSLEFFGLACKPS
                     QVPALLVMAAAAARVSKRKDGCEWASSSAFASVSFVTAERPERVLRGVLSPAQLSVTA
                     LLVEGKSYSEIASLRHTSERTIANQVAGTFRRLGVSGRAELLRYLVTSPRVFPETPPA
                     LCSLG"
     gene            complement(6061..7512)
                     /locus_tag="VFQ61_04665"
     CDS             complement(6061..7512)
                     /locus_tag="VFQ61_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate kinase"
                     /protein_id="HET9953766.1"
                     /translation="MTVNPAKLPRLAEQGASFLENLGHANRAFMRHYPGDRSSRQPVH
                     TVYGGAQLYKAETPKRLGELALSSLNSYAAGPVEFARGVGFLPEASLSQQDESAVRAQ
                     YAQDPERLRAANPAGWLACTVYERVVAKLEREPVEDFRIDFEDGFGARPDEEEDTVAQ
                     RAASELARAMSQGLTPPFIGIRIKSFSEEWKVRATRTLDLFLDTLLTHTGGKLPADFI
                     ITLPKVTLPEQPATLVRLLELLEQRHGLAPGSLRFELMIETTQALLGADGRSPLPSLL
                     IASEGRCIGAHLGTYDFTASCNITAAYQTMDHPMCDLAKGLMLLAYGYTGIFLSDGAT
                     NVMPVPVHRGVELSAEQRAQNKSAVYSAWRLAHRHIRHSLQGGFYQGWDLHPAQLPVR
                     YATCFAFFLEGLHSASERLRNFIEKAAQATLVGDVFDDAATGQGLLNYFLRALNCGAI
                     DLRDMAQTGLLPEEIELRSFAKILAARRTRLSP"
     gene            complement(7587..7964)
                     /gene="uraH"
                     /locus_tag="VFQ61_04670"
     CDS             complement(7587..7964)
                     /gene="uraH"
                     /locus_tag="VFQ61_04670"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HET9953767.1"
                     /translation="MTRITTHVLDVATGEPAVGLLVRLARLAGDTWLPLGEARTDQDG
                     RVSAFTSPESVRNAGGAVPEPGRGSEPPQYRLSFDARGYFASSQRASFYGWIEIAFYD
                     QGSDHYHVPLLLSPYGYSTYRGR"
     gene            complement(7961..8488)
                     /gene="uraD"
                     /locus_tag="VFQ61_04675"
     CDS             complement(7961..8488)
                     /gene="uraD"
                     /locus_tag="VFQ61_04675"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF010372.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HET9953768.1"
                     /translation="MSEVTLTAPLIELNRCAVADAERELLRCCGSTRWAQNVAAQRPF
                     SSLAALHEAAQRCWCEASETDCREAFSHHPQIGANLEELRKKFAPTASLSAAEQAGAA
                     HASETTLFELRELNLRYLERYGYIFIICASGRSAEQMLEALRARLDNEPDRELVLAAA
                     EQGKIMHLRLEKLFG"
     gene            complement(8481..9554)
                     /gene="alc"
                     /locus_tag="VFQ61_04680"
     CDS             complement(8481..9554)
                     /gene="alc"
                     /locus_tag="VFQ61_04680"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HET9953769.1"
                     /translation="MSEPQATGATFTGLVDLASSLLGSQPVGCSDDYFAEAHRMLQPG
                     RAQFIPGKYTDRGKWMDGWESRRKRGPGHDYCIIELGVPGRVQAFDIDTQHFVGNHPA
                     FASVEGAQLPPGIPLAQLQNAPFYELLGQSPLLPGSQNLFVAATPNPQLPGAVVTHLR
                     LNIFPDGGVARFRAYGQVATAFQSTSSPQQPALDEVESAQVPLGLVDLAALRNGGTAL
                     ACSDAHFGGMNHLLLPGRAENMGSGWETRRRRGPGYDWILIRLAARGVPRVIEVDTHH
                     FKGNFPDRCSIETIDAAGARPTDLIASRDFHLLLPETRLYADQRHFFRSELRAPAPAT
                     HLRLNIFPDGGVSRLRVWSEIIV"
     gene            complement(9551..>10506)
                     /locus_tag="VFQ61_04685"
     CDS             complement(9551..>10506)
                     /locus_tag="VFQ61_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9953770.1"
                     /translation="GELARSGVLGCKAFLVHSGIDEFPQTSEQDLREAMPILREAGLP
                     LLAHAELDLGPDSDSASADARNYRTYLASRPPSWEDEAIRLLVKLCRELRCPVHIVHL
                     SSATAIPILRAARAEGLPITVETCPHYLCLEAEQVPDGATHFKCAPPIRDHANREALW
                     SALLEGVIDFVITDHSPCLPALKLPERGDFLEAWGGIASLQLGLPAIWSEARRRGADL
                     ATLFRWMSSAPARFAGLSASKGRIDAEFDADLVIWDPDDTVPVDSEALFFRHKISPYL
                     GMRLEGRVHHTILRGASVYDGRQHPEPPRGKALLYRQASVA"
BASE COUNT         1970 a         3234 c         3368 g         1934 t
ORIGIN      
        1 gtcagggctc cccgactggc gcaccaccgc ggcgtgactc atcaggaaga agctgaagtt
       61 ctgttcctcc agagtcgggc aggtcgacgc tggcttccag ccgccgctgc tggccgtcgg
      121 tgtgggcggt ccgctggtgg ggggctcgtt cgaggacggt tcgctagaag actgcggcgc
      181 gcacgcgagc agccatccgc agattccaaa tccgaaacac tgctgaagaa caccgaacat
      241 cctgcgccgg aagttcgagc cgcttcgctg cctcgacatg cctcgaatgt agctggttcc
      301 tccgccaact cgcacagctg gggcggccat gtcgctggcc tggcaagacg gcggccacgg
      361 gttcgattta acgttcgcgc agcaaatgca cggcaaagcc agcgtttcgc accattttcg
      421 gcattcggtc cgccagtttg gggcgagatc cgtcatgcac gcttggaata ttatcgcaaa
      481 cgcacatgac tcgccccggc gcgcgtacaa tgctgagcgt ccctggagat acagacggag
      541 gtcgtccagt ggtgaaaccg aaccagcccg aagcgcaaga aatccaccgc gaactcgccc
      601 gctacatcaa tctcaaacta gcggcgctcg gcgaacccac tagctccgcc acggcagatg
      661 cttacttcat ggagttggca ggaccgctgt tgcggaacca cttccagaag gacgcactgc
      721 tcggctggcc actatgcccg gctgatagcc gcatccagag gttcctcgac gagtatctaa
      781 gcgaagtatg ccccgagggt gctccgcgca tcccggagaa gacgttcgct ctcgatcggc
      841 ccggcctggc tcgggtgctc tcgttgcccg tttctgccga tcgctttgcc tcgccattcc
      901 tgacctctta tcgggtgcaa cagggcgtgc tgcacaatcc gaagagtgat cggcgcacga
      961 cccaaggcgt ctttcacgtg acggagggcg ggctcccgat tccggcggat aagctggcgg
     1021 tcccgaagat caccttcgcg cgcttgctcg cggcggcgct gcggccgcca gaagcggtgc
     1081 tcaccttgcc gttcaccgcg gatcagccgc agccagcgca gctgttcgtg tccctactac
     1141 tcaggccttt ggtcattccg gagaccgaga acgagcccgc gaagagcatg gaaatccgct
     1201 tcttcgcgcc gggtagcttg gtgagcaatc tcgatttcgt agagggcatc ttcggcaacg
     1261 gcggagacgc gttcctgccc gagaacgacg ctgcgctcga tgtgaagagc ttcaccggcc
     1321 acagcggttg cgtgatcgtg gcgccgcaca tcgtgggtct gcccaagaag gcgctcggct
     1381 tgccccatta cgaccaagcc acggagcgtc aacgccgcga tggcatgtgc taccgcgacg
     1441 agaacgagcc ctacaacggg ggcaatgcgt tcaagatcac ttgtcgcgac gagcgcggcg
     1501 tgatggtcac cgtcattgcg gataactact acgggtattg caagaaagag gtcaaaactc
     1561 agatcagcta tgccgcgaac ctttacggcg cctgtgaaga ggagcacgcg ggtggcgcgt
     1621 tggcattccc gacctacgta attggtcagg acttctatgc ggatcgcagc ttcgtgttga
     1681 agaacacgtc gttcgaacgg gccatggaaa tcctgggctc gcgcgtagag gtaaagccca
     1741 accgttacgc cgtcgatcgc aagtatccgg acgtgttcta cgtgccggaa gactcggagt
     1801 tccacgtcgc ggacggccaa gtgcgctgga agtaccaggg cgcggtgcag accctcacgc
     1861 tgcgtacggg ggatatctac gtgctgccct ccggctatcg cttgcggctc gagaagcagc
     1921 tcggtgcgtc tctgattcgg ctcgtggcct gccgccccga cggcgtgttg tgccacaagc
     1981 cgtgcaccgt atcgggcggc ggcaagtccg agatttcgaa gtctctgaag ccgatgatcc
     2041 agcccgcgcc gatcttcgtg aaagagtatc agcgcgacat ggacgtggtg gcggagatac
     2101 tcaagcgaga ctttacgaag atctacaaag atcgcgcccc cgacgagcgc gcccgtcgcc
     2161 cgctgctcgg gctcgatcgc tctctgggct ccgtgatcca gctgctcacg ccctctcccg
     2221 agtacaccga cgagtacaac gcttgggtgc gcgcgctgcc gcagacgatt cgccagctgg
     2281 tgttcgtgct caagcgctat taccgccccg agtggggtga tgattggcgc tcacacttca
     2341 cggtcgatcg catcaacggc tatcccggtc acgagctgaa gttcaacaat caacgcttgg
     2401 tcggtagcta cttgcgcgtc gggttcgagc cgggttcgaa ctcttggcgt atgtacaagc
     2461 tgcgccctga cttcaacccg gccgacaaag tgcaggtcga ggacgacatc accgcatcgg
     2521 tagtggtgcc gcgtagccgc gtctctagct tcccggcggc ggagaagaac cagagcgtca
     2581 agatcgtgca gaactgcgag gcgttgctgt tccagcgccc ggacgacgcc attcatcgcg
     2641 gcttcgatca ccaagccgag gcggacttgg cgacgcccgg tacgttcatc accaacttcg
     2701 agccgctgcc gcgcgctcag gtgcaagcgc tcgccgacaa ggcggctgac ttcgatcgct
     2761 ttacggcccc catgaaggaa ctgctggagg gcttcctgaa ggcgccccag gccgaatacg
     2821 cggtctcgtc cgcccatccc cggatcgtgg atggcaagcc gtccaagaat ccgcgttacc
     2881 tgcagcgtcg ccccgatctc gtgcagcatc gcgaggctta cgtggccgag atcggccaac
     2941 gtctgcacga agagattccc gagcagaagc cggtgcccgc cgcggtgcag gccgtgctcg
     3001 cgggacggcg cgcgagcccc ccgcagcctg aaattgggct gcctccgttg gcggtctaca
     3061 gcccgattca ctatcaagag ctgcccgagc tgttcatgga cttcctgagc agcttgaccg
     3121 gcaagtcgcc ttccacgact ggcttcggca gcgaaggcgc gctcaccaaa gggccgttca
     3181 acgcgctgtg gccggtagtg gacatgaaca acgcgctgct ctcgttcatc ctgacgggct
     3241 actcgggatt cacgaccgcg gccggcttca ttgggccgaa ctacaaggtc gaccacgaca
     3301 tcagcatgct cgtgccagag atctggtgcc gcatgcgcac cgaggagcgc gacgcccggt
     3361 atctgatcga ccacggctat ctcgagaagg tgaacgactt cgacttcgag tcgcgccggg
     3421 tgctcgcgag ccgcctcggc tatcgcatca cgccgcgctt cgtggatcac tttctgggcc
     3481 gtctgttcga gaccccgaac acggtgttta cggaggaaat gctgcgtccg gagaagcaga
     3541 gcctggagat gttcgcgggc ggtgtcgacg cgatcgtcga gacccaaact cgcgtggcac
     3601 gcagctactt cgaagatggc agcgtggatg cagcttgccc gccgctcaaa gcactcctcc
     3661 acatcatggt gcacggcagc ttcgacggca tgacgctcga gcaccccgaa gtgagaaagc
     3721 tgttcacgcg cgaagccacc ttggagagcg cctggtacaa ggagcggctc cgcaccaagc
     3781 aacagcgcga cttggctctg tatcgccgtc acgcggcctc gctcgaagag gtttcgccag
     3841 tggttcgcgg ttcgctcgcg ggcttcgacg ctcgcttgtc ctacgtgcag gagcggctcc
     3901 agcaagtcgc ctctccacgc tacgtggacg agctggtcgg taccctcggc gcggatccgt
     3961 ttcacctaca gttggcgcgc taggcccgcc aactcgctcc cgctagggag tcagtgaagt
     4021 tcgggctcga agcagggtcg cgcgatgttc gcgcggccct gcgccgcttt gtgccggggg
     4081 cgcagctcga gcccctcgat tcggcttcgc ccgccagggg agaactttcg cgtcagctct
     4141 cgtgaatttt gatttccgag agcgccaatc ggagttgtgt cgtgccgggg cgacgctcga
     4201 tcaaatagcc ggcttcggtc agcgcgtgtt tctccatgtg tcgtttgagg cgatgaacat
     4261 cgccgtcgac ctgttgtggg ctatacgcca gcgcgtcagc caaatcggac ttataaatcc
     4321 agccgcattc gccggcgggg cggcccgcgg cctgatcggc gagacgttcc ttcgccaact
     4381 gtaacagcag aagattgaag ctgcggctgc cgagggaata gctgcgctcg gcattgtgga
     4441 gggagatttg cacgaactcg cgctcgtgag acacgtcgaa ttcgatcacc gggtcacggt
     4501 aggcttcctc gtgcgcacag gtggcgttca cgagctgagg gcagacgaag cgatagcgct
     4561 ggccttccag ctccagtacc gcgttgggct cgaccagctg ccgccgttgc ggctctgttc
     4621 tcacgggctc actggaggag ccgtacaacc accatctgcc gtctgctgac tggcgcactt
     4681 cgtgtcggac acccgagctg gtgctcagct cgatgcggcc gccgttttcc gggattgcga
     4741 cccgtgtctg ctcacaaacc agcatcgtca ccgggggact cgcgtctatt aggatccatt
     4801 gctcggcttg tccgaagcag atcacggatc cctcgtgcaa gtcgtgtgcg tagccgcgtt
     4861 cgatgcgctg cccgtcgacc cgcgtcccgt tgcggctgcc gcgatccaga atttgccaga
     4921 gatatccggt ccaacgaatc acgcagtgct ggcctgaaac gtacttggcc tggagccaca
     4981 gcgcgcattg cgggccgcga ccgaccaggt gctcgggctc gagaaaacat tccaacgtac
     5041 cttcgacgcg gcgaatccta cccatgggac tttcgtagat tagagcacga ctttgggcgg
     5101 agcgggacag gcttgagatg aactgcaatc gacgtctgca tcgaggtctg ggacgatgca
     5161 gacaaatcaa gggtggagcg aagcgagccg acgaaacatc gagggcattg cgatgaattc
     5221 agatctggac gcagaacaaa caagcagcgg aaacgcctgg cagccttggg gccaagtgct
     5281 ggagaatcaa ctccagatct acggtcgcgg ggagccgagc gaaatcgaag aggaccggac
     5341 tttgctagcc actcgggtac tcgaactcga gttgatatgg cctttgctca aagctgggct
     5401 tatcaaagtt tcaagaacac tctttaccag tacccatacc tcgtttctgc tggcgcccgg
     5461 agcggcggca cgagccaaaa agtcactccc cgagcgttac gccgaaatcc tcgagcgtac
     5521 gctgctggac tcgggcccga aggtggttgc tgcggagttg ggctattcga tatccggaat
     5581 tggcaccatc ttgcggcaga gcctcgaatt cttcgggctc gcgtgtaagc cgagccaggt
     5641 tcccgcactg ctggtgatgg ccgcggcggc ggctcgggtt tctaagcgca aggatggttg
     5701 cgagtgggcg tcgagttcgg cttttgccag cgtcagcttc gtgacggcgg aacgccccga
     5761 aagggtgttg cgcggcgtgt tatccccagc acaactcagc gttacggcgc tcttggtaga
     5821 aggcaagagc tactccgaga tcgcgagttt gcgacacacc tcggagcgaa ccatcgctaa
     5881 ccaagttgcc ggcacattta gacggcttgg tgtctccggt cgagccgagc tcctaagata
     5941 cctcgtaacg agcccacgag tgttcccgga aactccgccg gcgctctgct cgttgggctg
     6001 agtctagagg ccctcggggg acagaatcga ccccggcgtt tgtcgacgct cacttcgagt
     6061 tcagggagag agtcgcgtgc ggcgtgcggc gaggattttg gcgaagctgc ggagttcgat
     6121 ctcttcgggc aaaaggcccg tctgggccat gtcgcgaagg tcgatggctc cacagttcag
     6181 ggctctgagg aagtagttga gtaggccttg gccggtggcg gcatcgtcga acacatcccc
     6241 gaccagcgtg gcttgtgcgg ccttctcgat gaagttacgc aggcgttcac tagcgctatg
     6301 caacccttcg agaaagaacg cgaagcaggt ggcataccga accggtagct gagcggggtg
     6361 tagatcccaa ccctgataaa agccgccttg cagggagtgt cgaatgtgac ggtgagccag
     6421 gcgccacgcg gagtaaactg ccgacttgtt ctgggcacgc tgttcggcgg agagctcgac
     6481 gccgcggtgc accggcacgg gcatcacgtt ggtggcgcca tccgacagga aaatgccggt
     6541 gtagccgtat gccagtaaca tgaggccttt ggcgaggtca cacatggggt gatccatcgt
     6601 ctggtaggcg gccgtgatat tgcatgacgc ggtaaagtcg taagtcccca ggtgagcccc
     6661 aatgcagcgc ccctcgcttg cgatcagcag gctgggtaaa ggactgcgac cgtcggcgcc
     6721 cagcagagcc tgggtggtct cgatcatcag ctcgaagcgc aaggatcccg gggccaagcc
     6781 gtgccgttgc tccagtagct cgagcaggcg aaccagcgtg gcgggctgtt ccggtagggt
     6841 aactttgggc agcgtgatga tgaaatcagc ggggagcttg ccgcccgtgt gcgtcagcaa
     6901 tgtatccaaa aacagatcga gcgtgcgtgt ggctcgaacc ttccactcct cactaaaaga
     6961 cttgatccga atcccgatga agggaggcgt gaggccctga ctcatggccc gtgcgagctc
     7021 gctcgcggcg cgctgggcca ccgtgtcttc ttcctcgtcg gggcgagcgc caaagccgtc
     7081 ctcgaagtca atccggaagt cttcgacggg ctcacgctcg agcttggcta ccacccgctc
     7141 gtatacagtg caggcgagcc atcccgctgg attcgctgcg cgcaggcgtt ccgggtcctg
     7201 agcgtactgg gcgcgcacag ctgactcgtc ctgctgactc aggctggctt cgggcaaaaa
     7261 gccgacgccg cgtgcgaact ccactgggcc agccgcgtag ctattcagac tcgagagcgc
     7321 gagctcaccg agacgcttgg gggtctccgc tttgtagagc tgggcgcctc cgtacaccgt
     7381 gtgcacgggt tggcgtgacg agcgatcacc agggtagtgc cgcatgaaag cgcgattggc
     7441 gtgccccagg ttttccagaa aagaagcgcc ttgctcggcg aggcggggga gtttggcggg
     7501 gtttacggtc atgcttggcg ggcctgctga ggggtgaatg gcggattggg atcagggcga
     7561 ttggcgaagg gaacgaggca gcgcgcctaa cgcccgcgat acgtggagta tccgtagggg
     7621 ctgagtagca acgggacgtg atagtggtcc gagccttgat cgtaaaaggc aatctcgatc
     7681 caaccgtaga aggacgcgcg ttgggagctc gcgaagtagc cccgggcgtc gaagctcaaa
     7741 cgatactgag gcggctccga accccggccg ggctccggaa ccgcgccgcc cgcgttgcgg
     7801 accgactccg ggctggtaaa cgcgcttacg cgtccatcct ggtccgtccg cgcttccccc
     7861 aacggcagcc acgtatcgcc cgcaagacga gctaggcgta cgagcagccc caccgcgggt
     7921 tccccagtgg caacgtccaa tacgtgagtc gtgatgcgag tcatccgaat agcttctcca
     7981 aacgcaggtg catgattttg ccctgctcgg cggcggcaag gacgagctct cggtcgggct
     8041 cgttgtcgag cctcgcccgt aacgcctcca gcatctgctc cgcgctgcgt ccactggcgc
     8101 agatgatgaa gatgtatccg tagcgctcga gatagcgcag gttcagctcg cgcagctcga
     8161 atagagtggt ctcggacgca tgggcggcgc cggcttgctc cgccgctgac aggcttgcgg
     8221 tcggcgcgaa tttctttcgg agctcttcca ggtttgcgcc gatctgcggg tgatgcgaga
     8281 aggcttcccg gcaatcggtc tcgctcgcct cgcaccaaca gcgctgggcc gcttcgtgca
     8341 gggctgcgag cgaagagaac gggcgctgag cggcgacgtt ttgggcccat cgcgtagacc
     8401 cgcagcagcg caggagctca cgctccgcat ccgccaccgc gcagcgattg agctcgatca
     8461 acggagctgt gagcgtgacc tcagacaatg atttcgctcc acactctcaa tcggctgact
     8521 ccgccgtccg gaaagatgtt caggcgcaag tgcgtggcgg gtgcaggggc acgcagctcg
     8581 gagcggaaga agtggcgttg gtccgcgtag aggcgagttt cgggcaagag cagatggaag
     8641 tcacggctcg cgatcaagtc agtggggcgc gcgcctgcgg cgtcgatggt ctctatcgag
     8701 cagcgatctg ggaagttgcc cttgaaatgg tgggtgtcca cctcgatgac tcgcggaacg
     8761 ccgcgggcgg caagccggat caggatccag tcgtacccgg gcccgcggcg gcgtcgcgtt
     8821 tcccaccctg agcccatatt ttcggcgcga ccgggcagca gcaggtgatt catgcctccg
     8881 aaatgcgcgt ccgaacacgc cagtgccgtg ccgccgttac gcagggcggc caggtcgacc
     8941 agaccgagtg gaacctgggc gctctccacc tcgtccaacg caggctgctg gggcgatgac
     9001 gtagactgga acgcggtcgc gacctgaccg taggctcgga agcgggccac tccgccgtct
     9061 gggaagatgt tgaggcgcag atgcgttacc acagccccag gtaactgggg attgggcgtc
     9121 gcggcgacga acaagttttg cgagccgggg agcagcgggg attgccccag tagctcgtaa
     9181 aaaggagcgt tttgcagctg agccagcggg attccgggcg gtagctgagc gccttcgacc
     9241 gatgcaaagg cgggatgatt gccgacgaag tgctgggtgt cgatatcgaa ggcctgcacc
     9301 cttccgggaa cgcccaattc gatgatgcag tagtcgtgac cggggccgcg cttgcgccgg
     9361 ctctcccaac catccatcca cttgccacga tccgtgtact tacccggaat gaactgcgct
     9421 cggccgggct gcagcatgcg gtgtgcttcg gcaaaatagt cgtcgctgca gcccacgggc
     9481 tggctgccaa gcagtgagga agccaaatcg accaagcccg tgaaggtagc gcccgtcgct
     9541 tggggctcac tcatgcgaca ctcgcttgcc gatagagcag cgctttgccc cttggcggtt
     9601 cagggtgctg ccggccatcg tatacggacg ctccccgcag gattgtgtgg tgcacgcggc
     9661 cttcgaggcg catgccgaga tacggcgaga tcttgtgtcg aaagaacagc gcttccgaat
     9721 cgacgggcac ggtgtcatcg ggatcccaga tcaccaaatc ggcgtcgaac tccgcgtcga
     9781 tgcggccctt gctcgccgac agcccggcga accgcgcggg cgccgagctc atccagcgaa
     9841 ataacgtggc caggtccgcg ccacgccggc gcgcctcact ccagatcgcg ggcaacccca
     9901 actgaaggga cgcgatgccc ccccaagctt ctaggaagtc gccgcgctcg ggcagcttca
     9961 gcgccggcag acacgggctg tggtcggtga tcacgaaatc aatcacgcct tcgagtagcg
    10021 cgctccacag cgcttcgcgg ttggcgtgat cccggatggg aggcgcgcac ttgaagtgag
    10081 ttgcgccatc cggcacctgt tcggcctcca agcacagata gtgcgggcat gtctccaccg
    10141 tgatcggtaa accttccgcg cgcgcggcgc gcaagatggg tattgccgtc gcggacgata
    10201 gatgaacgat gtgcacgggg cagcgcagct ctcggcacag tttcacgagc aaacgaatgg
    10261 cttcgtcctc ccagctcggg gggcgcgagg cgagataagt tcgatagttg cgtgcatctg
    10321 cgcttgccga gtcagagtcg ggccccaagt cgagctcggc gtgggccaac aaggggagac
    10381 cggcctcgcg caggatcggc attgcctctc gcaaatcctg ctcactcgtc tggggaaact
    10441 cgtcgatgcc ggagtgcacc aggaacgcct tgcagccgag cacccccgag cgcgcgagct
    10501 ccccga
//
LOCUS       DASTKU010000132         4960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_472036, whole genome shotgun sequence.
ACCESSION   DASTKU010000132 DASTKU010000000
VERSION     DASTKU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472036"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1703
                     /locus_tag="VFQ61_04690"
     CDS             270..1703
                     /locus_tag="VFQ61_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HET9953771.1"
                     /translation="MHRLLPPRAYTLVSSIGIGLSGLILAASCREEAPACAAGSETCE
                     CLPNNTCQIGLTCLSQICVDLRTGKATGGRSGMGGQTSSAIAGTSTSGGQASSGGSSS
                     SALPPTGNTGGVTSPSGMTGGSSNVAGSGGTQTGGTPNTGGGGSGGASGGSGGSGAMA
                     NTPVGRHGALSVSGTKLLDASGAQVKLEGVSSMWLNWDPTGYAENKDGMRYMRDNWSM
                     RLFRAAMGVDAEGAYFADQNHAKSQVNTIVQNAIDLGIYVIIDWHDHNALDHKSEAIT
                     FFTEMAGKWGSYPNVIYEVFNEPLALDWNSQLKPYHEELVKAIRDKDPDNIIVLGTPN
                     WDQDVDAAAQSPVSGKNLMYALHFYACSHQGPIMDKAKAAFDKGLPMFVTEWGATHAD
                     GGTDGVVCEQPTRDWHSWLDSKMISWAAWKFDGCGDSSCFFKNRDVPTNGNWKADQLN
                     GHANLVIDLMRTGPGGEPSSSTPALGN"
     gene            1910..>4960
                     /locus_tag="VFQ61_04695"
     CDS             1910..>4960
                     /locus_tag="VFQ61_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HET9953772.1"
                     /translation="MNDEFVSRTLELSAEAWLVAGVAACELKVTESGFARLKLDAAFR
                     ECTGRRLLQEGGRVSTFIPGRAPGREASFEMPSEFRALTLRELARRGRLDEVIAVAKV
                     CVGERSVAPFTTALYSGNLSQLEKRAKTLKLCFRDGTEWEFARERLSEAVLTDFTPQQ
                     FEATFGEGTQEVATQVLGDALQDLSEVSELYSWGKRSRLEPSRKTQPLAQVLWDQALL
                     RGDADALKEQHPHFDTGTQYLLNAVRCYVQQDWALAQRAFADGLAWRRGANTRPLPAG
                     ALGVLGLLAIESGDPQTLTILKSWLRPTRARASAALGGFPAPDPAAAVARGLRTLLRY
                     EGLPEIERPRLDAHQVPARTGAWEVFLLGLTAHVHQGADLVRVAWARRLAALRERAEA
                     AGYGWFSAQAKALATALEQSHPDLAFREQNPTKSEHPKSESAKEEGVEREVLERSPLA
                     LVHLPKPRPVWRQTLESIEAWASDIEKRQLPNLRRVAWFIDLRSGTLSKPGLEEFLSD
                     QGWTMLRRLDSDELSSIERELPEEDRVVVRALAGKDAERGALEALCGHPRVFNGVRGR
                     AKVDVVHGTCKLITERDADRVRVRFTPHDPEPGVHVVVEGESRVVVYRVSEDVAKLAR
                     LLGSGLELPAQHQAELVRLLGRMVEHVELESPFLSPRSVTADASPCVRISPSAGAFWV
                     EVGVRPFGPEGRFFPPGRGATSVSAYTARGHLTSERSLAEELSRHEHFIRSCPSLVTA
                     RQGLQEEPSMVPGEQPFQFAFGEEELLELLQEFRGLTPAPAIEWQNTRAVSSRGKLSL
                     AQLKGGLRRNKGWYLVSGTVRLDDVTEMSLAELSRLPFTRSGKFLRLPSGDFVEVERR
                     MQRVLAALARTLPAGAPQSSTQELRLADADFQTLQLLAEADSGFELAAEARAALSKME
                     QSFRAEAPLPDGLAATLRPYQLEGYQWLCRFSELGIGVCLADDMGLGKTLQVIALLLR
                     NKQVATSSPRCLVVAPTSVCGNWVLELARFAPQLRVREYTGKDRA"
BASE COUNT          884 a         1486 c         1728 g          862 t
ORIGIN      
        1 cgagcgctaa ttgcggaggg tggccgcggg gctgcggccc tccgcgcgag catgagcata
       61 ggctgtaatc gtgccgtgtg tcggttcgag cagcgagcgt ccgcgttgga tcgccagctt
      121 gagaataacc ggcaagacgg ccgcgaacta gctgcggaaa cggtcccaca tcgttccaaa
      181 agcaacaaca atggcctcgc gcacttgccg aggtgcatgc tggaggatag ggtcggcagg
      241 tgtcgtcgtc tcgggcttcg cggatgagca tgcatcgact tctcccacca cgcgcctaca
      301 cgctcgtctc ttcgataggg atcgggcttt cgggcttgat tctcgcggcg agctgtcgcg
      361 aggaggctcc tgcttgtgcg gctgggagcg agacctgtga atgtctcccc aacaacacct
      421 gtcagattgg tttgacgtgt ctgtcgcaga tctgcgtgga tttgcgcacg ggcaaagcca
      481 ccggtggacg cagcggaatg ggtgggcaga catcgagcgc gatcgcaggg acgagcactt
      541 ccggtggcca agcgagctcc gggggttcgt cgagctcggc gctgccaccg accgggaaca
      601 ccggcggcgt gacttcaccc tctgggatga cgggcggaag cagcaatgtc gccggcagcg
      661 gcggcacgca aacgggcggc acgcccaaca cgggcggcgg tggttccggt ggagcgagcg
      721 gcggaagcgg tggctccggg gctatggcca acacgccggt gggtaggcat ggagcgctct
      781 cggtttccgg gacgaagctg ttggacgcgt ctggcgccca agtgaagctc gagggcgtga
      841 gctcgatgtg gctgaactgg gaccccaccg gctacgccga gaacaaggac ggcatgcgtt
      901 acatgcgcga caactggagc atgcgcctgt tccgcgcggc gatgggtgtc gacgccgaag
      961 gggcctattt cgcagatcag aatcacgcca agagccaggt caatacgatc gtgcaaaacg
     1021 cgatcgatct cgggatttac gtgatcatcg attggcacga tcacaacgcg ctcgaccaca
     1081 agtcggaggc catcacgttc tttacggaga tggcgggcaa gtggggcagc tatcccaacg
     1141 tgatctacga ggtgttcaat gagcctttgg cgctcgattg gaactcgcag ctcaagccgt
     1201 atcacgagga gctggtgaag gcgattcgcg ataaagaccc cgacaatatc atcgtgctcg
     1261 gaacgccgaa ttgggatcaa gacgtggacg cggccgcgca gagccccgtc agcgggaaga
     1321 acctgatgta cgcgctgcat ttctacgcct gctcgcatca ggggccaatc atggacaagg
     1381 cgaaagccgc cttcgacaaa ggcctgccga tgttcgtgac cgagtggggc gccacgcacg
     1441 ctgacggtgg tacggacgga gtggtgtgcg agcaaccaac acgcgattgg cacagttggt
     1501 tggactcgaa gatgatcagc tgggcggcct ggaagttcga cggttgcggc gatagctcct
     1561 gtttcttcaa gaaccgggac gtgccgacca acggaaactg gaaggcggat cagctgaacg
     1621 gtcacgccaa cttggtgatt gatctgatgc gtaccggccc tggcggcgag ccgagcagct
     1681 cgacgcccgc gctcgggaac tgatgagtca cggaccgccg gggtccgcgc aggcaacagc
     1741 cgcgcaggca acagcctcgc aggcgacagc ctcgcaggga accggcgcgg tcgcggcaga
     1801 gacccacgcc gaacgcccac ggcgcgacgc agaatccgcc gcgcggagcc ttctcgctcg
     1861 gaacggcttg cgagcggccg tctccgcgcg aacactgcgt ctttcgcccg tgaatgacga
     1921 gtttgtttcc cgtacgctcg agttgtcggc agaggcctgg ctggtggccg gcgtggccgc
     1981 gtgcgaattg aaggtgaccg agagcggctt cgcacgtctg aagctagacg cggctttccg
     2041 tgagtgcacg ggccgcaggc tgctccaaga aggcgggcgc gtgtctacct tcatcccggg
     2101 gcgcgcgccc gggcgagagg cctcgttcga gatgccgagc gagtttcgcg cgctcacgct
     2161 gcgtgaactc gcccgccgtg gacgtttgga cgaggtgatc gcggtcgcca aggtgtgtgt
     2221 cggcgagcgc tcggtggccc cgttcacgac cgccttgtat tccggcaatc tgagtcagct
     2281 cgagaagcgc gccaaaactc tgaagctgtg tttccgagac ggcacggagt gggaattcgc
     2341 gagagagcgc ttgagcgaag cggtgctgac ggatttcacg cctcagcagt tcgaagctac
     2401 gttcggcgaa ggtacgcaag aggtcgccac tcaggtgctg ggggacgcgc tccaggatct
     2461 atcggaggtc tctgagctct attcctgggg aaagcgctct cgactcgaac cgagtcgaaa
     2521 gactcaacca ctcgcgcagg tgctgtggga ccaagctttg ttgcgcggcg acgccgatgc
     2581 gctcaaagag caacaccccc acttcgatac tggaactcag tatctgttga atgcggtgcg
     2641 ttgttacgtc cagcaagatt gggcgctggc gcagcgcgcg ttcgcggacg gcttggcctg
     2701 gcgccgcggc gcgaacactc gaccgcttcc ggccggtgcg ctgggagtcc tggggttgct
     2761 ggcgatcgag agcggcgacc cgcagaccct cacgatcctc aaatcgtggc tgcggccaac
     2821 gcgggcgcgc gccagcgccg cgctcggagg ttttccagcg ccggatccgg ccgccgcggt
     2881 ggcacgtggg ctccgcacgt tactgcgtta cgaaggactc cccgagatcg agagaccgcg
     2941 gctcgacgcg catcaagtac cagcgcgcac gggcgcttgg gaggtcttct tactggggct
     3001 gacggcgcac gtgcaccaag gcgccgatct cgtgcgggtc gcttgggcgc ggcgcctcgc
     3061 ggcgctccgc gagcgcgccg aggccgcggg ttatggctgg ttttcagcgc aagccaaggc
     3121 gctggcgacg gcgctcgagc aatcccaccc agacctggcg tttcgcgagc agaatcccac
     3181 gaagagcgag catccgaaga gcgagagcgc aaaggaggag ggtgtcgagc gcgaggttct
     3241 cgagcgctct ccgctggcgc tggtgcatct gcccaaacca agaccggttt ggcgacaaac
     3301 cttggagtct atcgaggcct gggcgagcga catcgagaag cgtcagctgc ccaatttgcg
     3361 gcgcgtggcg tggttcatcg acttgcgctc gggcacgctg tccaagccgg gcttggagga
     3421 gttcttgagt gatcaaggct ggaccatgct gcgacgcttg gactcggacg agctctccag
     3481 tatcgaacgc gagttgccgg aagaggatcg cgtcgtagtc cgtgcgctgg ccggaaaaga
     3541 cgcggagcgc ggtgcgctcg aggccctgtg cgggcatccg cgtgtgttca acggcgtgcg
     3601 cggcagagcc aaagtagatg tggtgcacgg cacctgcaag ctgattaccg aacgagacgc
     3661 agaccgcgtg cgcgtgcggt tcacgcccca cgaccccgaa ccgggggtgc acgtggtcgt
     3721 ggaaggtgaa tcgcgggtgg tcgtgtaccg agtgagtgag gacgtcgcga agctcgcgcg
     3781 cctgctcggc tcaggcctcg agttacccgc gcaacatcag gccgagttgg ttcgcctgct
     3841 cggacgtatg gtggagcacg tggagctcga gagcccgttt ctctcgcccc gctccgtcac
     3901 ggcggacgcc tcgccttgtg tgcggatttc accctcggcg ggtgcgtttt gggttgaagt
     3961 gggagtacgg ccgttcgggc ccgaagggcg cttctttccg ccggggcgcg gggcaaccag
     4021 cgtctcggcc tacaccgccc gcggacactt gacgagcgaa cgatccttgg cggaggagct
     4081 gtcgcgacac gagcacttca tccgcagctg ccccagtctg gtgaccgcac gacaaggcct
     4141 gcaggaagag ccctcgatgg tgccgggaga gcagcccttt caatttgcgt ttggcgagga
     4201 ggagctgctc gagctgttgc aagagtttcg agggctcacc ccggcgcctg cgatcgagtg
     4261 gcagaatacg cgagccgtga gttcgcgcgg caagctgagt ctggcccagt tgaaaggtgg
     4321 actgaggcgc aacaagggct ggtatctggt ctccggcacg gtgcggctgg acgatgtgac
     4381 cgagatgtct ttggcggagc tctcgcgttt gccatttacg cgctccggca agtttctgcg
     4441 cttgccgagc ggagatttcg tcgaggtcga gcgccgcatg caacgcgtgt tggcggcgct
     4501 ggcgcgcacg ctgccggccg gcgccccgca gagcagtacc caagagctgc gcctcgcgga
     4561 cgcggacttt caaactctgc agctccttgc cgaagcggat agtggcttcg agctggccgc
     4621 cgaggcacgt gcggcgttgt cgaagatgga gcagtcgttt cgcgcggaag ccccgctgcc
     4681 cgacgggctc gcggccacgc tgcgtccgta tcaactcgag ggataccaat ggctctgccg
     4741 gttcagcgag ctggggattg gggtctgcct cgcggacgac atgggcctcg gcaagacgct
     4801 ccaagtaatc gcgctgctcc tgcgcaacaa gcaggtcgct acgtcttcgc cgcgctgtct
     4861 agtggtcgcg cctacttcgg tgtgcggcaa ctgggtgctc gagctcgcgc gattcgcgcc
     4921 ccagctgcga gtacgcgaat acaccggcaa agatcgcgcg
//
LOCUS       DASTKU010000133        22871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1140306, whole genome shotgun sequence.
ACCESSION   DASTKU010000133 DASTKU010000000
VERSION     DASTKU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 22871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22871
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140306"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFQ61_04700"
     CDS             <1..364
                     /locus_tag="VFQ61_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953773.1"
                     /translation="RHASLMAFQKSGAQGCGEQSGRRALPWLEHVPGYCVLGGEGTGS
                     SPYTKLRGARSTPRKRLEIRYITAGFDGLCDRPRSGRPPEIHPRFSCRHEGALREEEP
                     GVRALRRTRAASQYDVDE"
     gene            1183..1827
                     /locus_tag="VFQ61_04705"
     CDS             1183..1827
                     /locus_tag="VFQ61_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9953774.1"
                     /translation="MDNERLLFLPGASGNTEFWRPVAQRLSHPGERGFISYPGFGGVP
                     ADPNVRSFDDLISRVAGQITGPVSLLAQSMGGTLAVPAALVKPELVRSMVLTVTSGGV
                     DVTALGAQNWRPIFRRNNPHLPTWFEEHREDLSERIRQIRAPVLLLWGDADPISPVAV
                     GERLAKLLPCAELVVLRGGTHDLVLERSEEVVGQIDRFYARVRATKSANSQHPE"
     gene            2145..4763
                     /locus_tag="VFQ61_04710"
     CDS             2145..4763
                     /locus_tag="VFQ61_04710"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HET9953775.1"
                     /translation="MNRSSSRTPDDRDQSQSSAGARSLRFRLIGIFALAVAYFVTGRL
                     GLLLAIPPGYATAIWPPSGIALCALLVYGRGLWPGVLLGSFAVNAVVSSDLSTALTLV
                     RSVGISASIAGGAALQAWLGTTLIRARVGFPHALSEGRDVLRFLALGGPVACSVNAVV
                     GPGVLLLSGAFAPGALITNCLTWWSGDVLGVVVLVPVLLTFFGRPLEIWKPRRRSLAL
                     PMAAVVLVAILVFIRTSSLEQSQIQAQFQERVLTLERAVSDRLETHLQLLQSTRDFFG
                     ASREVVRDEFRRYVMPTLRSRPGLLAMEWVPRISRAERDSLEHAAHLDGLAGWRVRVF
                     PGEELERPLYYPIFYGEPMSRVQPALGLDLGSEPGRRAALEKARDLGVPTASSRVRLA
                     LTTETTDGILLLMPLYAGEGVPRTVEQRREGLRGFVVGVLQLDELVQSAIGPPRGREI
                     SVSIEDITTGSGASTLYAEPNRSAPKPLVAAALQQIGGRRWRLSFSSTAAYLAEYRSW
                     QAWAVLAAALLFGGLLGAFLLVLTGRNAQLERTSHEATQLLATIVQSSHDPIISYDPQ
                     GRIATWNSGAEKVFQWAASEVIGESFAKCLPEPALAEHRTRLAQVMAGEVLPTYETLR
                     ETKSGQALEVAVKLSPIYDMFGGVRGASEICRDISAEKNASRLLRASLREKELLLKEI
                     HHRVKNNLQVISSLLSLQAEHVETPGFTNALAESQARIQAIALVHDQLYRSRDLAQID
                     FAGYVRELLQSVLRVQNQGVRVTSKVTIDDVRLGVDAAIPCGLIVNELVTNALKHAFP
                     NRAEGQIEIALKRSGADAVKLCVADDGVGLPENFDPRQCKTLGLELVYTFAEQLEAQV
                     QIQRGPGTAFHITFSV"
     gene            complement(4755..6185)
                     /locus_tag="VFQ61_04715"
     CDS             complement(4755..6185)
                     /locus_tag="VFQ61_04715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9953776.1"
                     /translation="MVVRGKVADAEPARILVVEDEPIVAKAIRTSLGRFGYEVVSSVD
                     TGQKALAAAAQVRADLVLMDVHLKGDLDGIKTASELGRQYGLPVVFLTAYTDDETLQR
                     ASSVNALGYIVKPFNEATLKGSLKVALEKSRKDAEDHRRLSFLEKQVEDWNHPASQAK
                     MRLASIPSILPDSNGRRSQELERLGSALYVAAEGVARLNAEGRYIYANQAYAREHGYD
                     KNALIGQYGHVSIDAGDRVEYEVARGSMRNFGKSELECRGLRADGSSFFKHLTLVAIH
                     RHGSFEGHYEFCKNITQRKTVESELRELTIRDGLTGLLNRRELDRVLVEQCAMASIVA
                     EPLCVVLMDLDHFKSVNDRFGHVAGDAILVGVSQRLRSALRDVDLLARYGGEEIAIVL
                     PGTSAGEAATRAEALRVILSETPFAIRAHDGAEHLVRITASLGVAERKPQELNPELLL
                     RDADAALYRAKREGRNRVVVATERSD"
     gene            6279..6857
                     /locus_tag="VFQ61_04720"
     CDS             6279..6857
                     /locus_tag="VFQ61_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953777.1"
                     /translation="MSHRHPGHNEVYVSTDVEADGPIPGPHSMLSFGSAAYTAEKGLL
                     GTFSRNLETLPGAAPHPKTEAWWKTQPEAWAACRQNLVSPDSAMQNFVGWLRGLPGKP
                     VFVGYPAAYDFMFVYWYLMRFVGESPFSHSALDIKTFAMCQLKIGYREATKKGMPQRW
                     FDPVPHTHVALDDAIGQGALFCNMLAESRNRS"
     gene            6999..8447
                     /locus_tag="VFQ61_04725"
     CDS             6999..8447
                     /locus_tag="VFQ61_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HET9953778.1"
                     /translation="MTCTGRFRSVLGFGLLIGAFHSACGSTKTESAPNRAGSTSGVGS
                     IAGAAGNPGGGSKGEQTSGGASLMPRSAGGAPDTSAATETGGAAPSGGAPNGGAMDAG
                     TSREGEKGGSAPVARGGGTTSSSADHGGRTMVAGSGGTSSTGGSSSANQSGGTPQLAT
                     PQIPARPRPTVETESFSLGALADIADDSAIYANPSAPELSVVLADNKDDSAGGIAVFD
                     MQGKLLQLRKEGKIGNVDLREGFSLGGQTIVLVGANNRTHDSIQFWRLDPSTRQLSNP
                     IGEAKTSNAPNYGFCLYHSAATGKFYAFVTQETGASVIEQYELTESGGRLMASKVRAF
                     EVGSITEGCVADDELGRLYVAQEDVAIWRYDAEPSGGSQRTRVASVGDGHLVADLEGL
                     GLAKGAGTSGYLVVSVQSESRFAIYDRESNAYLRGFSISANADIDAPDQTDGLDISTV
                     NLGPGFPHGALIVHDGENANGATSNLKYIPLE"
     gene            complement(8546..9148)
                     /locus_tag="VFQ61_04730"
     CDS             complement(8546..9148)
                     /locus_tag="VFQ61_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953779.1"
                     /translation="MEAMLVKQIEELVLQSLEHEIGGTKVYQSAIACAVNPQLRQEWE
                     KYLAETEQHVVMLEKLCGALGIDAARETPGRAVVRHLGAALVQAMEMARGVGDPAAAQ
                     LVACECVVNAETKDHLDWHLIQKCAERVEGFAEEAVAKLLDEIEDQEDEHLYHSKGWC
                     RELWLESLGFEAVLPPPEERRHVKTALGAAKAEQAAEKVR"
     gene            complement(9154..11502)
                     /locus_tag="VFQ61_04735"
     CDS             complement(9154..11502)
                     /locus_tag="VFQ61_04735"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HET9953780.1"
                     /translation="MSGPATSESTALSDALADKFEGAQALAESFPFNAAKAAEYGRLA
                     IEPPPGATAEPPDLSATASTLTESMSSVKVGTRAQPGASPASLPLDRVRADGSGQRLT
                     TNQGVPIADNQNSLKFGLRGPALLEDFILREKITHFDHERIPERVVHARGSGAHGYFE
                     CYKPLTKVTRASIFGEAGKRTPVFVRFSTVIGERGSTDTARDVRGFAVKFYTDEGIWD
                     LVGNNIPVFFIQDAMKFPDLVHAAKPEPHFQVPQASTAHDTFWDFVSLMPESTHMLMW
                     VMSDRAIPRSYRMMQGFGVHTFRFVNAQGASTFVKFHWRPLLGTHSLDWDEAVKISGA
                     DPDFHRRDLWEAIEAGAFPEYELCVQLFTEEQAEKFSFDVLDATKLVPEELVPLTPIG
                     KMVLNRNPDNFFAETEQVAFCTAHVVPGIDFTNDPLLAGRIHSYVDTQITRLGGPNFH
                     EIPINSPVAPVQNNQRDGFHRQAIHRGRVAYEPNSLAGGCPFQAGRMAGFTSFREPAE
                     DEASKVRGKPERFAEHYNQARLFYNSQTAVEKAHILRAFRFELTRVQVPAIRERVVSM
                     LANVDTEFARALAADLGMDELPEPMPRVLKRAPKAEVEQSPALSLYALPGDGRIQTRR
                     VAIILADSSDVEAAEAIHAQLTELGAVPRYVGARLGSISSGKKGRRIEIEATFETMPS
                     VLFDAVAIPDGKMAIKTLANVGHALEFLKDQYRHAKPILALGAAADLLEGAAISPVLP
                     SGKKDPGVLVYKQEPLEKVIPQFVKAIARHRHHEREMDPPEV"
     gene            complement(12249..14816)
                     /locus_tag="VFQ61_04740"
     CDS             complement(12249..14816)
                     /locus_tag="VFQ61_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9953781.1"
                     /translation="MMQYGKTALVGFLTVVAVSNGCSDSPGSAGNSAGNAGAGAGATG
                     NGGRSSEDPGGRPSGGGTSSGASAGTQSLGGAATGGLDSGGRSSGGVGGLDGGGSAGT
                     AGDGGAKPNRGGATSGGTADNGGASDGGATEPNIGGASEGGTTNTGGSAGMAGWDAGR
                     CMLPIGVPPTMPVRSAVTWAADVGDFNADGHLDMAVTGENFDRRHVVSVRLGLGNGVF
                     ASPGEEFGVEKNPKALAVGDIDADGKLDIVTANYDANSVSILLGRGDGRFEPATSLPV
                     GSQPVSLAIADVNGDRRADLLVACQGSSDVRILVGTVSGSFTALSSLSVGEQPLSLSV
                     ADVSGDGTLDLLVGAWTASTSNVHVLLGTGGATFASAVKYPVPFGPYGVATGDFDENG
                     KLDLVAAASEGSTIAILSGTGKGAFSTQPTTYSVGVGPMALSVRDFDKDGHLDIASAN
                     YTSHDITILNGAGNGQFPTNATYFVGGNPRTLVLGDVSDDGAVDIVLSHGQTLLADGN
                     GAFAAQARITAGSKPSAVAVGDFNADRKLDLAVANNASKTVGISLASASYTFPAPTSY
                     TVGNDPVALAVGDLTGDGALDIIAANKSDGTLSVLAGSSTGVFTVRSPVSAGGGVNAL
                     ALGDIDHSGTLDIVAANDVGGNVSILLGAGGGALAQPKTIAVSSTTNGVALGHFDADD
                     HLDVIATVNYSLVRNTQLLFGNGDGSFELGPGVLEDALLAVTTGDFNGDGRLDYVTLG
                     EVGAFVHLSVGDGTFLPPTRVYASSVPSALAVGDLNRDGRLDLVLTQSTNLAISVYLG
                     LGDGTFETGLDYPAGPKPAGLALGDLNGDGWLDVVTANQDGNTVGVLWNRGIVCGVR"
     gene            15390..16097
                     /locus_tag="VFQ61_04745"
     CDS             15390..16097
                     /locus_tag="VFQ61_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HET9953782.1"
                     /translation="MKMRVYAAPIALALVSFACSAAGSDETSLEPSVDAVESSLIISS
                     NQTGTNNGYHYQFWKDGGNVSMDLLNGGQYQVSWQAGNYNFVGGKGWKTGSARTVNYN
                     AGVWSPGNSNAYLTVYGWFQNPLIEFYIVDSWGTWRPTQGTKMGTVTCDGGTYDVFRS
                     QRVNAPSIEGNKTFNQWWSVRTSKRATGSNASINTSCHINFWKSKGLSIGTWNYMVMA
                     TEVFNPGSSGMSNLTVW"
     gene            16321..16749
                     /locus_tag="VFQ61_04750"
     CDS             16321..16749
                     /locus_tag="VFQ61_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET9953783.1"
                     /translation="MLGHCLCGKVEFEVAADHLKLYQCHCSLCRRQGGSSSNTAGIVP
                     NARFRWLRGSAGIATWQKESGFRSDFCSRCGSPVPNPLRGLPYFWVPAGLLENDAQLE
                     IVAHLCVGSKPVWDSTSLRGPCFEHLPNLPEFIALLGVDG"
     gene            16876..17838
                     /locus_tag="VFQ61_04755"
     CDS             16876..17838
                     /locus_tag="VFQ61_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953784.1"
                     /translation="MKRLELAPGIELTSFQRGDDVASGPVAFDAGADGRLYVVYASNP
                     QAYRAVHGGGASSAVARPRKPQSYRVLALNELGVELDVSIASEPFNIHHVQPLGELLL
                     LACARCRYSSSGNHDRNGRVYTREGKLVDELVLGDGIQDLQTTRSGLIWTSYFDEGVF
                     GNFGWSTPLGSAGLVAWDGTGRKVHEFRATSGLEPICDCYALNVVSNEEVWLSYYTDF
                     PLVQLKSGRVFKHWPAPTRGSSAFAIGNGHCLFRGGYDERGSYILGRLGSSENLTVIQ
                     RFALFDEAGAPLEPTRTSARGDKIYLLCGTQVYALDLAKAFGVV"
     gene            complement(17878..19074)
                     /locus_tag="VFQ61_04760"
     CDS             complement(17878..19074)
                     /locus_tag="VFQ61_04760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9953785.1"
                     /translation="MRAQTRTPHMLGYSAALSLALGAIALTSACSSDPASTVPGSALG
                     GATAATGGASAAGGTSAQGGAAAGGSSATGGISGQGGASTTGGAATSGGTTSASNGGS
                     SASSGGGTGAAGGASGGAAGASTGGKSSIGGSGAGGTSAGSGGSSSSGGSSGGGKSST
                     GGAGGTSGGSTATGGSGGGTGGAGGAGGASGFNPCPPAGTPCKVLPLGDSITDGIGFS
                     GGYRVELFHLANTDKKSITYLGGSMNGPSQVDGVTFPKNHEGHSGWTIQQIDNIVPSP
                     ALQTTPHIILLHIGTNDMYQTPSGAPDRLSTLIDQIVMAAPEALLVVAKIIPFPQQSG
                     QVTTFNTGVGTVVDAKIKAGKHLILVDQFTGFPTSELGDGVHPNQAGYSRMAKVWYEA
                     IKTYLH"
     gene            complement(19206..20117)
                     /locus_tag="VFQ61_04765"
     CDS             complement(19206..20117)
                     /locus_tag="VFQ61_04765"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9953786.1"
                     /translation="MLHDRALSSIDLNLLVVLRALLSERHVTRAAARVGLSQSATSHA
                     LSRLRELYGDPLLVRSGRALALTPRAARLLPALERGLDELRGTVIGDSEFVPSSARRT
                     FAIGMSDYLQVLVMGPLLRQLAAQAPGVDLSVVVFSNVRERIESESIDLALSIPGAET
                     QGLREERLFEEDFTCIVRRDHPEIRRRLSLEKYLDQRHVVVAPGGTPGSVVDAVLAQR
                     GLERRIALRVTNFLIAPVVVSETDLINTMPSRLARQVAKTYPLRLLPPPLELPRFDYR
                     MYWHPRLDHDPAQRWLRDFIARVSRAC"
     gene            20202..21059
                     /locus_tag="VFQ61_04770"
     CDS             20202..21059
                     /locus_tag="VFQ61_04770"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HET9953787.1"
                     /translation="MFVITGATGHTGSVAARALLEAGRKVRVVVRDAAKASRLKSLGA
                     EVFVADLADQAALAEAVRGAEGVFLISPPDLTAKDFVAERQRLTQQQIETLKAAKVPH
                     VVLLSSIGAQQPSGTGPILTTHNAEQQLRASGLSATFVRAGYFVENWAAVIPAVKNDG
                     VLPSFIAADQRVPSVSTVDIGKAIAQALLDGPRGVRVIELSGPTDVSPNEVAATFARI
                     LGKPVHVAQAPLDAVVPTFKSFGLSENIAGLFRELYEGLANGRIVPEPGEHVRGTTPL
                     ESTLRALLA"
     gene            complement(21099..>22871)
                     /locus_tag="VFQ61_04775"
     CDS             complement(21099..>22871)
                     /locus_tag="VFQ61_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9953788.1"
                     /translation="IRGSGYADVDFVIPEVVRELHVREGVYDPRQGDAAIVGSADFSL
                     GVARRGTRVKLSYGSFNQLRALGIVAPRGANQDTFVAVALRKTDGFGARRASRSASLN
                     AQYGFELPNRFQMRMFVAGNTAESELAGVVREDDVASGRIGAFDSYPYFAENQGVAAH
                     RILAGVEFTRRERGGTKLRLLPFISASHLRARQNYAGTLETSQIDPSLAARGDLFETR
                     NRELMGGALFSVRTERRLGPKTRIVIEPGLQARLGSTRLGKNLLEPSTLGVWDRRIDA
                     AVTSLDLGAYFDVELTLWNRLRIAGGPRLDYLARTIDDQLTYALFRSGQTPGGTRRSA
                     AGAFVGPRLSAEYWLAPELSLMAAYGEGFRSLDPSRLPEGNTRPYSRVRSIEAGLRAR
                     DPSGLLSSSIAVFRTLVGNELVFAPEAGGLDTQNASVRSGLVTSLLLRSHVGLLVSTA
                     LSVTEAQYQTRVAGISHVVPNVPPLLWRADVSWEHGLGAVAAKPLIGHLRAGYTWAAP
                     AHISDQLRGPGTHALAVGADLRREGVRVGFDIYNVLDRRSADSADRFVSNWGFSPGQQ
                     PASMAVHTVAAPPRTLVASLELEL"
BASE COUNT         4129 a         7241 c         7207 g         4294 t
ORIGIN      
        1 tcggcacgct tctttgatgg cttttcagaa gtccggagcg cagggctgcg gggagcagag
       61 cggacggagg gctctcccgt ggctcgagca cgtacctggc tattgcgtgt tgggtggaga
      121 aggcacgggc tcatctccat acacgaagct ccgcggcgcg cgtagcacac cgcgcaagcg
      181 gctggaaata cgatacatca ccgcaggctt cgacgggctc tgcgaccgcc cacgctccgg
      241 tcgtcctccc gaaatccatc ccaggttctc ctgcaggcac gaaggtgctt tgcgcgagga
      301 agaacctgga gttcgagcac tccgtcggac gcgagcggcg agccaatacg acgtggacga
      361 gtgacctgca tcgaatgtga atacgcgtgc ggaggcacac gcgaccgcgt gctgctttgg
      421 gtgacagccg atagcaccac gccaacggca ctcagcttcc tcgcccctat caaccactac
      481 aggcgaggat ccatcgtcat caacactgac aacatcagca cacgaactgg agatgctgcg
      541 gccgcacggc tcgcgaagca cccgcgggta cgcttcgttt tcacgccaac ccacggcagc
      601 tggctcgatc aggcggagag ctagttcggc attctcacgc gctccgccct tcggcgccgc
      661 tcttttgcgg ccgtgagtga gctacctgct gcaatctata agttcggcaa acactggaac
      721 aatgtcctcc ggcacccctt ccaatggact tacaatggtt tgggcacaaa gcgagcaacg
      781 cttgcgagga tttgatcggc cgtgttggtc caaatgaggg gtttcgcgct tccttggtgg
      841 gcgtcgatgt agtgacggat ggcttcctcc aactcggcac cgcaggcacg acggtgcttt
      901 gacgcactcc gccggacgcg agcggcgagc gcaaggcgac gtggacgagt gactcgcatc
      961 gaacttgaat ccgagcgcaa aggcacccgc aacatcttcg ccgcttccaa tatccgcgac
     1021 ggcggcgtgc tacgggtcgt cgaggtgtgc agcgtggcca gccgatgcgg ggaagctgaa
     1081 tcatccagga tgggcgccgc gggccatctc acgagccgtc gagcccgaga ctagctctgc
     1141 taggcctcca cgcgccattg tagtagtgat agcgcggtcg agatggacaa cgagcgttta
     1201 ctctttttac cgggcgcttc cggaaatacc gaattttgga gacctgtcgc ccagcgtcta
     1261 tctcatcccg gggagcgcgg cttcatctcc tatcctggtt tcggaggtgt gccggccgac
     1321 ccgaacgtac gcagtttcga tgatctgatc tcacgggtcg cgggtcagat caccggtcca
     1381 gtgagcttgc tcgcgcaatc catggggggc acgctcgcgg tgccggcggc gctcgtgaaa
     1441 cccgagctcg tccgcagtat ggtgctgacg gtcacctcgg gtggcgtgga cgtgaccgca
     1501 ctcggcgcgc agaactggcg cccgatcttc cggcgcaaca atccgcatct tccaacgtgg
     1561 ttcgaagaac atcgggaaga tctcagtgag cgcattcgcc agattcgagc gccagtgctc
     1621 ctgctctggg gggacgccga tccgatcagc ccggtagcgg tcggagaacg cctcgccaaa
     1681 cttctgccct gtgcagagct cgtggttttg cgaggcggca ctcacgactt ggtgttggag
     1741 cgcagcgaag aagtcgtggg tcagatcgat cgcttctatg cgcgagttcg tgccaccaag
     1801 tcggccaatt cgcagcaccc cgagtgagtg gcagggtaca ctgcgtaagt tagtgtgggc
     1861 gagttggcgc gaacatctcg gcatgctgaa tagcctgtgc agccaatagc cggttcggct
     1921 gagacggtgc agtttgcacc gtcggtactc gacacgcgga attcgactcg gtgcatgcag
     1981 ccgggtccgg tgccgcgcgt tgaaccgacc ttgtttctct tctaacctgc tctattcagt
     2041 aaagcaggtt ggcggttcac gtggctggct ccgagagctc tcgaagtact cggttgcatg
     2101 cgctcgggcg cagcgcacca atcacgcctg ctcagaagga cagagtgaat cgctcgagct
     2161 cccgaacacc ggacgatcgc gatcagagcc agtcttcggc gggtgcgcga tccctccgct
     2221 tccgcttgat agggatcttc gccctggcgg tggcttactt cgttacgggg cggctcggct
     2281 tgctgcttgc gattcccccc ggctacgcca ccgccatctg gcccccttcg gggatcgcac
     2341 tatgtgccct gctggtctac ggcagagggc tctggcccgg ggtgctgttg ggctccttcg
     2401 cagtcaatgc ggtggtgagt agcgatctca gtacggcgct cactcttgtc cgttcagtcg
     2461 gcatctcggc gtctattgcg ggtggtgctg cgcttcaggc ctggctcggc acgacgttga
     2521 ttcgcgcccg cgttggattt ccgcacgcgc tgagcgaggg acgagacgtt ctgcgcttcc
     2581 tggccctcgg aggtcccgtt gcctgctcgg tgaacgccgt ggtgggccca ggcgtactgt
     2641 tgttgagtgg cgccttcgca ccaggcgcgc tgatcaccaa ttgcctgacg tggtggtcag
     2701 gggacgtgct cggggtcgtc gtgttggtac ccgtgctgct caccttcttc ggtcgtcccc
     2761 tcgagatctg gaagccgcgc cgacgctcct tggcgctgcc catggcggcc gtagtgctcg
     2821 tcgcgatcct cgtcttcatt cgcaccagct cgctggagca gtcacagatt caagcgcaat
     2881 ttcaggagcg cgtactgacc ttggagcggg cggtttcgga tcgcttggag acacatctgc
     2941 agctcctgca aagcacgcgc gacttcttcg gggcatctcg agaagtagtg cgggacgagt
     3001 tccggcgtta cgtgatgccg acgttgagaa gccgcccggg gttgctcgcc atggagtggg
     3061 tgccgcgtat ctcgcgcgca gagcgggata gcctcgagca cgcggcacat ttggacgggc
     3121 tcgcaggctg gcgggtgcgc gtcttcccgg gtgaagagct cgaacggccc ctgtactacc
     3181 caatatttta cggtgagccg atgtcccgcg tgcagcccgc gctgggtctg gatctcggct
     3241 cagagccggg gcggcgcgcg gccttggaga aggcgcgtga tctgggcgtg cctacggcga
     3301 gctcgcgggt gcggctggcc ctcaccacgg agacgacaga cggcatccta ctgttgatgc
     3361 cgctctatgc aggggagggg gtgccccgga cggtagagca gcgccgcgaa gggctgcggg
     3421 gtttcgtggt cggcgtgctg cagctggatg agctcgtaca gtccgcgatc ggcccgcccc
     3481 gagggcgtga aatcagcgta tcaatcgagg atattacgac tggtagcggg gcaagtacct
     3541 tgtacgcgga gccaaatcgc agcgcgccga agccgctcgt ggcggcggcg cttcagcaga
     3601 ttggcgggcg gcgctggcgc ctatccttca gctccaccgc cgcttacctg gctgaatatc
     3661 gaagctggca ggcgtgggcc gtgctcgcgg cggcgttact attcggaggg ctactcggcg
     3721 cgttcctgct cgtgctcacg ggccgcaatg cccagctcga gcgaacctct cacgaagcca
     3781 cccagctgct cgcgaccatc gtccaatcgt cccacgaccc catcatctcc tatgaccccc
     3841 aggggcgaat cgcgacctgg aactcagggg cagaaaaggt atttcaatgg gccgcctcgg
     3901 aggtgattgg cgagagcttc gcaaagtgct tacccgagcc ggcgctggca gagcaccgaa
     3961 cgcgtctcgc gcaagtgatg gccggcgaag tgctgcccac ttacgagacg ctgcgcgaga
     4021 ccaaatccgg tcaagctctg gaagtggcgg tgaagctgtc tccgatttac gacatgttcg
     4081 ggggcgtgcg gggcgcttcg gaaatttgcc gcgacatctc ggcggagaag aacgcgagcc
     4141 ggctgctgcg cgcttcttta cgcgagaagg agctgcttct gaaggagatt catcatcggg
     4201 tgaagaacaa cctgcaagtc atttcgagtc tgttgagctt gcaggccgag catgtcgaga
     4261 cgccaggctt cacgaacgcg ctagccgaga gccaagcgcg tattcaagcc atcgcgctag
     4321 ttcacgacca gctttatcgc tcccgggact tggctcaaat cgacttcgca ggctacgttc
     4381 gggagttgct gcagtcggtg ttgcgtgtcc aaaatcaggg cgtgcgcgtg acctccaagg
     4441 tcacgatcga tgacgtgcgg ctgggcgtag atgcggccat cccctgcggc ctaatcgtca
     4501 acgaactcgt gaccaacgcg ctcaagcacg ccttccccaa tcgagccgaa ggccagatcg
     4561 agattgcgtt gaagcgctcg ggcgcggatg ccgtgaagct gtgtgtcgcg gatgatggcg
     4621 tgggcctccc cgagaacttc gatccgcgcc aatgcaaaac gttgggtctc gagctagttt
     4681 atacgtttgc cgagcagctc gaggcgcagg tccagatcca gcgcggcccg ggcaccgcgt
     4741 ttcacattac cttctcagtc tgagcgctcc gtcgcgacaa cgacgcgatt gcgcccttcc
     4801 ctcttggcgc gatagagtgc ggcatccgca tcgcgcaaca acagctcggg gtttagctct
     4861 tggggcttgc gctcggccac ccccagactc gccgtgattc gtacgaggtg ctccgcacca
     4921 tcgtgggcac gaatcgcgaa tggcgtctca ctcaagatca cgcggagcgc ttccgcccgc
     4981 gtcgcggcct cacctgcaga cgtgcccggt aagacaatcg cgatctcctc gcccccgtag
     5041 cgtgcgagca aatcgacgtc tcgcagcgcg ctgcgtagac gctgtgacac tcccaccaat
     5101 attgcatccc ccgcgacgtg cccaaaccga tcgttgacgg acttgaagtg gtcgagatcc
     5161 atcaaaacca cgcacagcgg ctcggccacg atcgacgcca tcgcgcactg ttccacgagc
     5221 acgcgatcga gctcacgccg attgagcagt ccggtcaagc cgtcgcggat cgtgagctca
     5281 cgcagctcgc tctcgaccgt cttgcgttgc gtgatgttct tgcaaaactc gtaatggccc
     5341 tcgaatgagc catggcgatg gatcgcgacc agggtcaggt gcttaaagaa tgacgaaccg
     5401 tccgcgcgta agcctcggca ttcgagctca cttttgccga agtttcgcat cgagccgcgc
     5461 gccacctcgt attccacccg atcccccgcg tcgatgctga cgtgcccgta ttggccgatc
     5521 agcgcgttct tgtcgtagcc gtgctcgcgc gcgtaggctt gattggcgta gatgtaccta
     5581 ccttccgcgt tcaagcgggc cacgccttcg gccgcgacgt acaaggcgga gccgagccgt
     5641 tcgagctcct gactgcgtcg tccgttggag tcgggcagga tgctggggat ggacgcgagc
     5701 cgcattttgg cttgagaggc ggggtgattc cagtcctcga cttgcttctc caggaaactc
     5761 aaacgccgat gatcctcggc atcttttcgg ctcttctcca atgcgacctt gaggctgccc
     5821 ttcaaggtgg cttcgttgaa tggcttcacg atgtagccga gggcattcac cgaactcgct
     5881 cgctggagcg tctcgtcgtc ggtgtacgcg gtcaagaata cgacgggtaa gccgtactgg
     5941 cggccgagct ccgacgcggt cttgattccg tcgagatctc ctttgaggtg cacgtccatg
     6001 agcaccagat cggcgcgcac ctgcgccgcg gccgcgagcg ccttctgccc ggtatcgacg
     6061 gagctgacga cctcgtaacc gaagcgcccc aagctcgtgc ggatcgcctt ggccacgatg
     6121 ggctcgtctt cgacgacgag aatgcgcgct ggctctgcat cggccacctt tccgcgaacg
     6181 accatcggag cttcgagttt agccgtaccg agcacatcga gtgctctcgc gccgagtcaa
     6241 tttacctcgg gcattttacc ccgctaatat ggggcgtcat gtcacaccgc cacccgggac
     6301 acaacgaagt ttatgtgagc accgacgtcg aagccgatgg ccctattcca ggcccgcatt
     6361 cgatgctgag cttcggcagc gccgcataca cggccgagaa ggggctgctt ggcacgttct
     6421 ctcgtaatct ggagaccctg ccgggtgctg cgccccaccc gaaaacagaa gcttggtgga
     6481 aaacgcagcc cgaagcttgg gctgcttgtc gccagaacct ggtctccccc gactctgcga
     6541 tgcaaaactt cgttggctgg ctccgcggat tacccggtaa gccggtgttc gtaggatatc
     6601 cggccgctta cgacttcatg ttcgtttatt ggtacctgat gcgcttcgtt ggagagagcc
     6661 cgttctctca ctccgcgctc gatatcaaga cgttcgcgat gtgccagctg aagatcgggt
     6721 atcgcgaagc gacgaaaaag ggcatgcctc agcgctggtt cgaccccgtc ccccacacgc
     6781 acgtcgcgct cgatgacgcg atcgggcaag gtgccctgtt ttgcaatatg ctcgccgaat
     6841 cacgaaaccg gagttgaggt gctgctcgag gcttctcgcc ctcgcacccg atttcaaaga
     6901 agaacaggcc acgatcttcg aaacttactt tgcggtggaa agcggcgggg agctagggcc
     6961 attgcctgtt ttgggtcgca ccgcgctact gatccgccat gacctgtact ggccgcttcc
     7021 gttccgtact cggtttcggg ctactcatcg gggcctttca cagcgcctgc ggttcgacga
     7081 agaccgaatc cgctccgaac cgcgccggca gcacgagtgg agttggctcg atcgcgggcg
     7141 cagcgggcaa cccgggtggc ggaagcaaag gcgaacaaac cagcggaggt gcgtcgctca
     7201 tgcctcgttc agccggtggt gctccggaca catccgcagc gactgagacc ggcggagccg
     7261 ctccaagcgg aggtgctccg aacggcggcg ctatggacgc gggaacttcg cgcgaaggag
     7321 aaaagggcgg gagcgcgccc gtcgctcgag gtgggggcac tacatcgagc tccgcggatc
     7381 acggcggccg cacgatggtt gcgggtagcg gcgggaccag cagtacgggc ggctcttcga
     7441 gcgcgaacca gtcgggaggc actcctcagc tcgccacgcc gcaaatcccg gcgcggccca
     7501 gacccacggt ggagaccgag tcgttctcgc tcggcgcgct cgccgacatt gcggacgaca
     7561 gcgcaatcta cgcgaatccc agcgcgcccg agctcagcgt cgtgttggct gacaacaaag
     7621 acgattcagc cggtggcatc gccgtattcg acatgcaagg caagctcttg cagctacgca
     7681 aagaaggcaa gatcggtaat gtcgacctgc gggagggctt tagtctgggc gggcaaacca
     7741 tcgtgctcgt tggggccaac aaccgcaccc acgattccat ccaattctgg cgcctcgacc
     7801 cgagcacgcg gcagctctcc aatccaatcg gcgaagccaa aaccagcaac gcacccaatt
     7861 acggcttttg cctctatcac agcgcggcga cgggcaagtt ctacgccttc gtcacgcaag
     7921 agacgggcgc ctccgtgatc gagcaatacg aactcacgga gagtggcggc aggctcatgg
     7981 ccagcaaggt tcgcgctttc gaggtgggca gcatcaccga aggttgcgtc gcggacgacg
     8041 agcttggtcg cttgtatgtc gcgcaggaag acgttgcaat ttggcgctac gacgccgagc
     8101 ccagcggtgg atcgcagcgt actcgtgtcg cgtcggtcgg cgacggacac ttggtggccg
     8161 atctcgaggg cctcggcttg gccaaaggcg caggtacttc cgggtatctc gtcgtctcgg
     8221 tccaaagcga gagccgtttc gcgatctacg accgcgagag caacgcctac ctgaggggat
     8281 tcagtatctc ggcgaacgct gacatcgacg cgcccgatca aaccgatgga ctcgacatct
     8341 ccacggtcaa tctcggcccc gggttccctc acggcgctct gatcgttcac gatggcgaga
     8401 acgccaacgg cgccacttcg aatctcaagt acatcccgct cgagtgaatc gagcgcttcg
     8461 cgtcgacacg gagcgcaccg agcggagctg cttctgcaag cagccccgct ccgaagagag
     8521 agtgcgaggg cgggccggtt tgcgctcagc gcaccttttc tgccgcttgt tccgccttgg
     8581 cggcgccgag cgccgtcttc acgtggcgac gctcctcggg gggcggcagc accgcttcga
     8641 atccgaggga ctcgagccag agctcacgac accacccctt ggagtgataa aggtgctcgt
     8701 cttcctgatc ttcgatctcg tcgaggagct tggcgacggc ctcctcggcg aagccctcca
     8761 cgcgctccgc gcacttctgg atcaagtgcc agtccaagtg gtccttggtc tcggcgttga
     8821 ccacgcattc acaagccacc agctgggccg cggccggatc tcccactccg cgcgccatct
     8881 ccatggcctg tacgagcgcc gccccgaggt gccgaaccac cgcgcggcct ggcgtctctc
     8941 gcgccgcatc gatgccgagc gctccgcaca gcttctcgag catcacgacg tgttgctcgg
     9001 tttcggccaa gtacttttcc cactcttgcc tgagctgggg attgaccgcg catgcgattg
     9061 ccgactgata gaccttggtg ccgccgatct cgtgctcgag cgactgcagc accagttcct
     9121 cgatttgttt gactagcatc gcttccatct ccttcagacc tcggggggat ccatctcgcg
     9181 ttcatggtgg cgatggcgcg cgatggcttt gacgaactgg ggaatcactt tttcgagcgg
     9241 ttcttgcttg tagacgagca cgcccggatc ctttttgccc gaaggtagaa ccggcgaaat
     9301 cgctgctcct tctagcaagt ccgcggccgc gccgagcgcg agaatcggct tggcgtgccg
     9361 gtactggtcc ttgaggaact ccagcgcgtg accgacgttc gcgagcgtct tgatggccat
     9421 ctttccgtcc ggaatcgcga ccgcgtcgaa caacaccgac ggcatcgtct cgaacgttgc
     9481 ctcgatctcg atacggcggc ctttcttgcc gctcgaaatc gagccgagac gcgcgccaac
     9541 gtaacggggc accgcgccga gctcggtcag ctgcgcatga atcgcctccg cggcctcgac
     9601 gtcactgctg tcggccaaga tgatcgcgac ccgacgagtt tgaattcgac catcccccgg
     9661 cagcgcgtac agagaaagcg ccggggattg ctccacctcc gctttggggg ctcgcttgag
     9721 cacgcgcggc atcggctcgg gcagctcgtc catcccgaga tcggcggcca acgcgcgtgc
     9781 aaattcagta tctacgttgg cgagcatcga gaccacgcgc tcgcgaatcg cgggcacctg
     9841 aactcgggtc agctcgaagc gaaacgcgcg taagatgtgg gccttttcca ccgccgtctg
     9901 actgttgtag aaaagccgcg cttgattgta gtgctccgcg aagcgctcgg gcttgccgcg
     9961 taccttactc gcctcgtcct cggccggctc gcgaaacgag gtgaaccctg ccatgcgccc
    10021 ggcttgaaac ggacagccac ccgccagaga attgggctcg tacgccacgc ggccgcggtg
    10081 aatggcttgg cggtggaacc catcgcgttg attgttctga accggggcca ccggcgagtt
    10141 gatcgggatc tcgtggaaat tgggcccacc cagacgcgta atctgcgtat ccacgtaaga
    10201 gtggatacgc ccggcgagca gcgggtcatt cgtgaaatcg atgccaggca cgacgtgggc
    10261 cgtgcaaaat gcaacctgct ccgtctccgc gaagaagttg tccgggttcc gattgagcac
    10321 catcttgcca atcggggtga gaggaaccaa ctcttctggg acgagcttgg tcgcgtcgag
    10381 cacgtcgaag ctgaacttct cggcttgttc ctcggtgaac aactgcacgc agagctcgta
    10441 ttcagggaag gcgccggcct cgatcgcctc ccacaaatcc cgccggtgaa agtccggatc
    10501 ggcccccgag atcttgacgg cctcgtccca atccagcgag tgagttccga gcagcgggcg
    10561 ccagtgaaac ttgacgaaag tcgaggcgcc ttgcgcgttc acgaagcgga acgtgtggac
    10621 gccaaagcct tgcatcatgc ggtaactgcg tggaatggcc cgatccgaca tgacccacat
    10681 cagcatgtga gtcgactcgg gcatcaaaga tacgaagtcc caaaacgtat cgtgtgccgt
    10741 cgatgcttgc ggcacctgaa aatgcggctc gggcttggcg gcgtgaacca ggtccgggaa
    10801 cttcatcgcg tcctggatga agaacaccgg gatattgttg ccgaccagat cccaaatccc
    10861 ctcgtccgtg tagaacttca cggcaaaccc gcgcacatca cgggctgtgt ccgtcgagcc
    10921 acgttcccca atcaccgtag agaaccgcac gaatacgggg gtgcgcttgc ccgcctcgcc
    10981 gaagatcgag gcgcgggtca ccttcgtgag gggcttgtag cactcgaagt agccgtgggc
    11041 acccgagccg cgcgcgtgaa cgacccgctc ggggatgcgc tcgtgatcga agtgagtgat
    11101 cttctcgcgt aggatgaagt cttccagcag cgcgggccca cgtagcccga acttcagaga
    11161 gttctgattg tcggcgatgg gcacgccttg attggtcgtg agccgttgac cgctcccatc
    11221 cgctcgcacg cggtcgagtg gcaacgatgc ggggctcgct ccgggctggg cgcgcgtccc
    11281 cacctttacc gaggacatcg actcagtcag agtgctcgcg gtcgcgctga gatcaggcgg
    11341 ctccgccgta gctccgggcg gtggctcgat agcgagtcgg ccgtattcgg cggctttggc
    11401 cgcgttgaaa ggaaaggatt cagcgagcgc ctgcgcgccc tcgaacttgt cggcgagcgc
    11461 gtccgagagc gctgtcgatt cgctagtcgc gggcccgctc acggtagagc gcgcactcga
    11521 tgtagttggc gaagccgcgg cacgcttcct ttcagagccc ttgtctaatc ctctacgtgc
    11581 ggggttcgtc ggaggcgttt tcttcatacc catggtgact cccgtttaga agcaggcgcg
    11641 acgtgtgcac actgcgtgcc gcgctccgtt ctccgcgcct gcgctgttac tcgagtctac
    11701 tgcatggcct tggatttgct cgatgcaggg cgctctcatt cgatcgtgaa ctcacagtca
    11761 acgaagcaca tcggtttttc ggtgtgcgcg cgggttctgt ccgctctcca gaactgccgc
    11821 atcgtggcgt ttctgggtaa cgtgacagac actcgaattc acgcggcggc aatagagcgg
    11881 aatcgcgcta tcgcgtgctc gcgttcacgc acgtgatttc gcgcatgcac gtcgttgatc
    11941 tcccggcccg ccgccgatct gcgaaatggc tgcgggcgta ctctcgttgt ctcgattctg
    12001 tgcagggccc cccacgcggc gcggaggccg cgtgaggcgg ggccctgccg cgcgcagcgg
    12061 agcgagcacg gcagaacgtg tagtaagcac cacttggggg gcatccgggg ttttggtttg
    12121 aaaaggccgc gatgtaacgg gcgcgccgcg agccccagcc ctcgacagcg cagccgatga
    12181 accctcttga actcgcaata ccgcgccggc ccccctaaca acgccgcgcc acgttgagcg
    12241 aagtgtaatt acctaacgcc gcacacgata cctcgattcc agagcacccc gacggtattg
    12301 ccatcctgat tggcagtgac cacgtcgagc caaccgtcac cgttcaagtc gccgagcgca
    12361 aggcccgccg gcttcgggcc cgcgggatag tcgagtcccg tctcgaatgt tccgtcaccg
    12421 aggcccagat agacgctaat cgcgagattg gtgctctgag tcaatacgag atcgagccgc
    12481 ccatcgcggt tcaaatcgcc caccgccagc gcggagggaa cgctgctcgc gtagacccgc
    12541 gtgggtggga ggaacgtgcc gtcacccacg ctgagatgaa cgaacgctcc gacttcgccc
    12601 aaggtcacgt agtcgagccg gccgtctcca ttgaagtctc ccgtggtgac cgcgagcaag
    12661 gcgtcttcca agacgcccgg gccgagttcg aaggagccat ccccgttgcc gaacagcaat
    12721 tgagtattgc gcacgagcga gtagttgacg gtcgcgatca cgtcgaggtg gtcgtcggcg
    12781 tcgaagtggc cgagcgcgac gccgttcgtg gtcgacgaca cggcaatcgt cttgggttgc
    12841 gctagcgctc ccccaccggc tccgagcagg atgctgacgt tgccaccgac gtcgttggcg
    12901 gctacgatgt cgagcgttcc gctatggtcg atatcgccga gcgcgagcgc gttgacaccg
    12961 cctcctgctg acaccggaga gcgaacggtg aagacaccgg tgctgctacc agccagtacg
    13021 ctgagcgtgc catcactctt gttcgcggcg atgatatcga gtgcaccatc gccggtcaga
    13081 tctcccaccg cgagcgcgac cgggtcgttg cccacggtat aggaggtcgg agctggaaag
    13141 gtgtagcttg cggaagccag cgagatcccc acggtcttcg aggcattgtt ggcgacggcc
    13201 agatcgagct tgcgatccgc gttgaagtct ccgaccgcga cggcgctcgg cttggagccc
    13261 gcggtgatgc gcgcttgggc cgcaaacgcg ccgttgccgt cggcgagcag ggtttgcccg
    13321 tggctaagga cgatgtcgac cgcgccgtcg tcgctcacat ccccgagcac cagggtgcgc
    13381 ggattgcctc cgacgaagta agtcgcattg gtcgggaatt gcccgtttcc ggccccattg
    13441 aggatagtga tgtcgtgact cgtgtaattc gcgctggcga tatcgaggtg cccgtctttg
    13501 tcgaagtcgc gcacggagag ggccatcgga cccacgccca cgctataagt cgtcggttgg
    13561 gtcgagaagg ctcccttgcc agtgcccgac aagatcgcga tcgtgcttcc ttcggaggcc
    13621 gccgcgacga ggtcgagctt gccattctca tcgaagtcgc ccgtcgctac gccgtagggc
    13681 ccaaagggca ccgggtattt cacggccgag gcgaaagtgg ctccgcccgt tccgagcagc
    13741 acgtgaacgt tggaggtcga agccgtccag gcgcccacca gcaaatcgag ggttccatcg
    13801 ccgctcacgt cggcgacgct cagcgataag ggttgctcgc ccacactcaa cgatgagagc
    13861 gcggtgaacg agccggacac cgtgcctacc agaatgcgaa catcggacga accctgacaa
    13921 gccaccagga gatccgcacg gcggtcgccg ttgacgtccg cgatggcgag cgagacgggc
    13981 tgactgccga cgggcagcga agtggcgggt tcgaagcggc catcaccccg cccaagcagg
    14041 atacttacgc tgtttgcgtc atagtttgcg gtcacgatat cgagtttgcc gtccgcgtcg
    14101 atgtcaccca ccgcgagcgc cttggggttc ttttccaccc cgaactcctc acctgggctg
    14161 gcgaacacac cgttacccag gcccagacgc acgctgacga catgacgacg gtcgaagttc
    14221 tcgcccgtga cggccatatc gaggtgacca tctgcgttga agtcaccgac gtcggctgcc
    14281 caagtcactg cggagcgcac cggcatcgtc ggaggcaccc caatcgggag catgcagcga
    14341 cccgcgtccc agcccgccat tccggcacta cccccggtat tggtcgtacc tccctcggag
    14401 gccccgccga tgtttggctc ggttgcgccg ccatcgctag cgccgccatt gtcggccgtt
    14461 ccgcccgagg ttgcaccgcc gcgattgggt ttcgcgccac catcgcccgc ggtaccggcc
    14521 gaaccgcctc cgtcgaggcc acccactccg cccgaactcc ggcctcccga gtcgagacct
    14581 cccgtagcgg caccgccaag gctttgcgtt ccggcgctgg ctcccgaact cgtgccgccg
    14641 cctgagggcc ttccaccagg atcttcgctc gatcggccgc cattaccggt ggcccccgcg
    14701 ccggctcccg cgtttcccgc cgaatttcct gctgaaccgg gagaatcgct gcagccattg
    14761 ctgaccgcaa ccacggtcag gaaccccacc agcgccgtct tgccatattg catcatcgtc
    14821 cactccacac ggcaggctcc aaccctgccc caaacgggtc gaatatacgg caacgtcgca
    14881 gcgtggtgcg agcgcattca aagcggggcc gcaaatttgt gggtccacgc tcgtggccaa
    14941 ctccgagtgg ctacgggcgc gagtcgggcc cacccattgg tgcctctggc cgctactgaa
    15001 actctagcaa gcgtgagcgc ggccctcgcc attcaaggag ccatggttcg acttgctcga
    15061 gctctggacg gagccgaagg cgaaccgccg taggctagac ccgactcgga gcggagcctc
    15121 gtgtgattag gtcccacgcc ttagcgaggt gaagttccgt gtgcccgtga accccatagt
    15181 tctcacgcaa gagagtgtcg ttcgatgatt gaagactaag tgtcagtcgg tcccaccgat
    15241 tgattgacag cgtggcgatg gttgtaatac tcacacccaa ctcgccgcca cgagcttgct
    15301 tggtcctggt cgcagcacga ccgggaagac gtgactcgat atcgcgtgct cgtggtttct
    15361 gaccacctga tgggagaacg aggataccaa tgaaaatgcg tgtgtacgcc gcgccgatcg
    15421 cgctggcgct agtttccttt gcctgttctg cggccggaag tgatgagacg agcctcgagc
    15481 cgagcgtcga tgccgtcgag agctcactca tcattagctc gaatcagacc ggcacgaaca
    15541 acggctacca ttaccagttt tggaaagacg gcggcaatgt gagcatggat ttgctcaatg
    15601 gcgggcaata ccaagtgagt tggcaggccg gcaactacaa cttcgtgggc ggcaagggtt
    15661 ggaagaccgg cagtgcgcga accgtcaact acaacgccgg cgtttggtcg cccggcaaca
    15721 gcaacgccta cttgacggtg tatggctggt tccaaaatcc actcatcgag ttttacatcg
    15781 tcgacagctg gggcacctgg aggccgaccc agggtacgaa gatgggcacc gtcacttgcg
    15841 acggcggcac ctacgacgta ttccgaagcc agcgggtcaa cgcgccatcc atcgagggca
    15901 acaagacctt caatcaatgg tggagcgtta gaacctccaa gcgcgctacg ggcagcaatg
    15961 cttccatcaa caccagctgc cacatcaatt tctggaagag caaaggcctg tcgatcggga
    16021 cctggaacta catggtcatg gccacggagg tcttcaatcc cggcagcagc ggcatgtcca
    16081 atctaactgt ctggtgagtg agccgtgccg ctgagccgcg ccggcacggg cgcgaagccc
    16141 ggccgagcac atcaaaagcg cggtcgcggt gctcgcgggt acctcaatca ccgcgctggc
    16201 tcccctcgta aacgcgcctt catgcgcagt tgaacggggg tattcggcgg ggagacctcg
    16261 agttgccggg cgcgcacagt ggacgtggat gcgcggcggc ggtagcaaca gccaacgtca
    16321 atgctgggtc attgcctttg tggcaaagtc gagttcgagg tcgccgcgga ccacttgaaa
    16381 ctgtatcaat gtcactgctc gctgtgtcgt cgacagggcg gctcctcgtc gaacacagcg
    16441 ggcatcgtgc ccaacgcgcg cttccgctgg ttacgcggaa gcgcgggcat cgcgacctgg
    16501 cagaaggaat ccggcttccg ctctgacttt tgctctcggt gcggctctcc cgtgcccaac
    16561 cctctgcgag gcttgccgta cttctgggtt ccggccggat tactcgaaaa cgacgcccag
    16621 ctcgagattg tggctcacct ctgtgtgggc tcgaagccag tctgggattc cacatcgctc
    16681 cggggcccct gtttcgagca tttgcccaat ctccccgagt tcatcgcgct gctgggggtt
    16741 gacggttgag aaccgatccc atggggagcg ccgctcttgg cccgagttca cgagaggggc
    16801 gtccgcctat tcccatgggc tatgctcgtg agcggtggtg accgggcgcg aatgcggcgt
    16861 aatctcagtc agcacatgaa aagattggaa ctagctccgg ggatcgagct gacatcgttc
    16921 cagcgcggcg atgacgttgc cagcggtccg gtggcgttcg atgctggggc cgacggccgg
    16981 ctgtacgtgg tgtatgcgag caacccgcaa gcctaccgcg cggtgcacgg tgggggagcg
    17041 agctcggcgg tcgcacggcc ccgcaagcca cagagctatc gtgtcctcgc gctgaacgaa
    17101 ctcggcgtcg aactcgacgt cagtattgcc agcgagccat tcaatattca ccacgttcag
    17161 cctctcgggg aactgctgct gctcgcgtgc gcgagatgcc gttactcctc ctctggcaat
    17221 cacgaccgga atggtcgtgt ctacacgcga gaaggaaagc tggtcgacga gctcgtattg
    17281 ggagacggaa tccaagatct ccaaactact cggagcggac tgatttggac atcctacttc
    17341 gacgaggggg tgtttggaaa cttcggctgg tcgacaccgc tcggttcggc gggactagta
    17401 gcgtgggatg ggaccggcag gaaggtgcac gagtttcgag caacgagcgg cttggagcca
    17461 atctgcgact gctatgcact caacgtcgtc tcgaacgaag aagtttggct ttcctattat
    17521 acggacttcc cgctcgtgca gctgaagagt ggccgggttt tcaagcactg gccggctccc
    17581 acgcggggga gctccgcgtt cgcgatcggg aatggtcatt gcctttttcg aggtggctac
    17641 gacgagcggg gcagctacat cttggggcgg ctcggaagct cggaaaacct cacggtcatc
    17701 cagcgcttcg cgcttttcga tgaagcgggg gcgccgctcg agcccacgcg tacctctgcg
    17761 cgcggggaca aaatctacct actgtgcggc acccaggtgt acgcactcga tctcgcgaag
    17821 gccttcggcg tggtttgata tcgcgcggtt cagcggagaa gtcgcgcgaa atcccgatca
    17881 gtgcaggtaa gtcttgatgg cttcgtacca gaccttggcc atgcgagagt agccagcctg
    17941 gttgggatgg acgccgtcgc ccagttccga ggtggggaag cccgtgaatt gatcgaccag
    18001 gatcaggtgt ttccccgcct tgatcttggc gtcaacgacg gtgccgactc cggtgttgaa
    18061 ggtggtcact tggccagact gctgcgggaa ggggatgatc ttggcgacca cgagcagcgc
    18121 ctccggtgcg gccatgacga tttgatcgat gagggtgctc agccgatctg gagcgccgct
    18181 cggggtttga tacatgtcat tggtcccgat gtggagcagg atgatgtgcg gagtcgtctg
    18241 cagggcaggg ctcgggacga tgttgtcgat ttgctggatg gtccagccgc tgtgcccctc
    18301 gtggttcttg gggaaggtga cgccatcgac ttggcttggt ccgttcatcg agccgcccaa
    18361 atacgtgatg ctcttcttat cggtgttcgc gaggtggaat agctcgaccc gatatccccc
    18421 cgagaagccg atgccatccg tgatggagtc cccgagcgga aggactttgc acggagttcc
    18481 ggcgggcggg cacgggttga agcccgatgc ccccccggct ccacccgcgc cgcccgtacc
    18541 gccgccgctg ccgccggtgg cggtcgagcc gccgctcgtg ccgcccgcac cgccggtgct
    18601 cgacttgccg ccgccgctcg accctccaga gctcgacgag ccgccgcttc cggcactcgt
    18661 tccgcccgcc ccgctgcctc cgatcgagct ctttcctcct gtgctggctc ctgcggcacc
    18721 gccactcgct cctccggccg cgcccgtgcc gccgccacta gacgcggagc tgcctccgtt
    18781 cgaggcactc gtggttccgc cgctcgttgc cgctccgccc gtcgtcgagg cgccgccctg
    18841 gcccgatatc cctccggtcg cggagctgcc tccggcggct gcgcctccct gagccgacgt
    18901 gcctccggcc gccgatgccc cacccgtcgc tgcagtggct ccgcccagcg ctgaaccggg
    18961 gacggtgctg gctggatcgc tcgagcaagc gctcgtgagg gcgattgcac ccagggccag
    19021 gctgagagca gcagagtaac cgagcatgtg gggagttcgg gtttgagctc gcatgatcac
    19081 tctcggttcc tagacttcgc gaccctcccc gggaagcgtc attctagaca ccggtccgat
    19141 attgaatctt tccagtgtcg cggatcattc gaataccttg agtaaggtgg tgtctcgagt
    19201 atgggtcaac aggctcggct cacgcgcgcg atgaaatcac gcaaccagcg ttgagcgggg
    19261 tcgtgatcca accgagggtg ccaatacatg cggtaatcga acctgggtag ttcgagcggc
    19321 ggtggcagga gtcgcaatgg gtacgtcttc gcaacctggc gcgcgaggcg ggatggcatg
    19381 gtgttgatca aatcggtctc actcaccacg accggtgcga tcagaaagtt ggtaaccctg
    19441 agtgcgatcc ggcgctcgag ccctcgttgc gcgagaacgg cgtccaccac gctgccgggg
    19501 gtgccgcccg gcgcgaccac gacgtgtcgc tgatcgagat acttctcgag gctcagtcgt
    19561 cgacggatct ccggatggtc ccgtcgcacg atgcaggtga agtcctcttc gaacaggcgc
    19621 tcctcgcgca ggccctgtgt ctcagcgcca ggtatgctga gggccagatc gatcgattcg
    19681 gactcgatcc gttcgcgcac attgctgaac accaccaccg agagatctac gcccggcgcc
    19741 tgcgccgcga gttggcgcag cagtggcccc atcacgagta cctgcaggta atcgctcatc
    19801 ccgatcgcga aggtgcggcg cgccgagctc ggaacgaact ccgaatcgcc gatcaccgtg
    19861 ccccgcagtt cgtcgagacc acgttcgaga gcggggagca gtcgagcggc tcgcggggtc
    19921 agcgccagcg ctcgaccgct gcgcaccagc agcgggtccc catacagctc gcgcagccga
    19981 gaaagcgcat ggctggtggc agactgactc agtcctacgc gcgccgcggc gcgggtgaca
    20041 tgccgttcgc tgagcaaagc ccgcaagacc accagcagat tcagatcgat cgatgacagc
    20101 gctcggtcat gcagcatgtg catgactata gtgctgaact tgcattggac gcatcaaccg
    20161 attgcaccca tgctcacctc ctcagcaggg agaaaaacac catgttcgtt attactggag
    20221 ctacggggca tacgggttcg gtcgcggcac gagcgttgct tgaagcggga agaaaggtgc
    20281 gggtcgtagt ccgggacgcg gccaaggcct ctcgcctgaa gtcgctcggc gcggaggtat
    20341 tcgtggcgga tctggcggac caggccgcgc tcgcggaagc ggtacgcggg gcggagggcg
    20401 tgttcttgat ctctccgccc gatctcaccg ccaaggattt cgtagctgag cgtcagcgcc
    20461 tgacccagca acaaatcgag accttgaagg ccgccaaagt cccccacgtg gtgctgttgt
    20521 cctcaatcgg ggctcagcag ccgtctggca ccggccctat cctgacaacg cacaacgccg
    20581 agcagcaact gcgcgcttcc ggtttgtccg cgaccttcgt gcgcgctgga tacttcgtcg
    20641 agaattgggc ggcggtgatc ccggccgtga agaacgacgg agtgctaccg tcctttatcg
    20701 ctgccgatca gcgggtgccc tcggtcagta ccgtggatat cggcaaggcc atcgcgcaag
    20761 cgttgctcga cgggccacgc ggcgtgcggg tgatcgagct gagcggcccc accgacgtga
    20821 gcccgaacga ggttgcggcc acctttgctc ggattttggg taaacccgtg cacgtcgcgc
    20881 aagcaccgct cgacgcggtc gtgcctacgt tcaagtcgtt tggtctttcg gaaaacatcg
    20941 ccgggctgtt ccgcgagttg tacgaaggtc ttgcgaacgg acgcatcgtg ccagagcctg
    21001 gtgagcacgt tcgtggcacg actccgctcg agtcgacgct gcgcgcgctg ctcgcctgag
    21061 cgacgcctcg cggaaaatga ccgtaggtgg tcaaagctct agagctcgag ctcgagcgaa
    21121 gcaacgaggg tgcgcggcgg cgcggcgacg gtgtgaacag ccatggacgc cggttgctgg
    21181 cccgggctga agccccagtt agagacgaag cggtcggcgc tgtccgccga tcgccgatcc
    21241 aacacgttgt agatatcgaa tccgacccgg acaccctcgc gacgcaaatc cgctccaacg
    21301 gcgagcgcgt gggtgcctgg gccgcgaagc tgatcagaaa tatgagccgg cgcggcccag
    21361 gtgtaaccgg cccgcaaatg cccaatcaag ggtttggccg cgacggcacc caggccatgc
    21421 tcccagctca cgtcggctct ccagagcagg ggaggtacgt tgggcaccac gtgcgaaata
    21481 cccgccacgc gcgtttgata ctgcgcctcg gtgacggaga gtgcggtcga gacgagcagc
    21541 ccgacgtgcg aacgcaacaa caaggacgtg acgagtccgc tgcgcacact cgcgttctga
    21601 gtgtcgagcc cgccggcctc gggggcaaac acgagctcat tacccaccag ggtccgaaac
    21661 accgcgatgc tagaactcaa gagtccgctc gggtcgcgcg cgcgaagccc cgcctcgatc
    21721 gaacgtacgc gcgagtaggg tcgggtattg ccttccggca agcgacttgg atcgagtgaa
    21781 cggaagccct cgccataggc ggccatcaag gaaagctcgg gggcgagcca gtattcggcg
    21841 ctgaggcgcg gcccaacgaa tgctcccgcg gcggagcggc gcgttccgcc aggggtttgc
    21901 ccggagcgaa acaacgcata ggtgagctgg tcgtcgatgg tcctcgccaa gtagtctagc
    21961 cgtgggcccc ccgcgatgcg caggcgattc cataacgtga gctcgacatc gaagtaggcg
    22021 cccagatcca ggctcgtgac cgcggcgtct attcgccggt cccaaactcc gagtgtggac
    22081 ggctccagca gattcttgcc caagcgtgtg ctccccaagc gagcctggag gcccggctcg
    22141 atcacgatgc gcgtcttcgg tccgaggcga cgctccgtgc gaaccgagaa cagggccccg
    22201 cccatgagct cacgattgcg agtttcgaag aggtctccgc gcgcggcgag ggaaggatct
    22261 atctgcgagg tctcgagtgt gccggcgtag ttctggcgcg cgcgcaggtg actcgcgctg
    22321 atgaacggca acagtcgcag cttcgttccg ccgcgctctc ggcgcgtgaa ctcgacccca
    22381 gccaggatcc ggtgcgccgc cacgccctga ttctcggcga agtaggggta agagtcgaag
    22441 gcaccaatac gccccgaagc gacgtcgtct tcgcgcacga cgcccgccag ctcggactcg
    22501 gccgtgttgc ccgccacgaa catgcgcatt tgaaagcgat taggcagctc gaacccatat
    22561 tgggcgttca ggctcgcgga gcggctggcg cggcgtgctc cgaagccatc cgtcttgcgc
    22621 agtgcgaccg cgacgaaggt gtcttgattg gccccgcggg gggccacgat accgagcgca
    22681 cgaagttgat tgaacgagcc gtagctcagc ttcacgcgag ttccccgccg agccaccccg
    22741 agggagaagt ccgcgcttcc gacaatggcc gcgtcgcctt gtcgcgggtc ataaacccct
    22801 tcccggacgt ggagctcacg cacgacctcg ggaatcacga agtccacgtc ggcatagccg
    22861 gaacctctga t
//
LOCUS       DASTKU010000134         6184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_916782, whole genome shotgun sequence.
ACCESSION   DASTKU010000134 DASTKU010000000
VERSION     DASTKU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916782"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFQ61_04780"
     CDS             complement(<1..534)
                     /locus_tag="VFQ61_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953789.1"
                     /translation="MSLGPALASYLVAGRLAAQPAPPTEPLDDAEIEVAPGQAAPNTS
                     AAGGDTAAPPSNASAPSVREPPSAAPTPAPVTPSAPSTPSAPVGIATSPGAAPSGSIA
                     QQPDSSETHTAAQKTYTATEKAAPAPAPLVDVLGYAQLEYQSHQDSEEQLRQGGALLN
                     QDRFLVRRARLVFKRRWE"
     gene            complement(725..1252)
                     /locus_tag="VFQ61_04785"
     CDS             complement(725..1252)
                     /locus_tag="VFQ61_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953790.1"
                     /translation="MSSNRFGLGMVLFVSSALCAFVGCGDDDDNPTPSKGGSQSSGGK
                     TSKGGASSGGDTSDAGESATGGTGGTTSKGGASNTGGKSSTGGKASGGEPPVEGGAGA
                     QGGETSTGEGGAGNSTSLSDCDEPNSKGCYEDCKPVEDAHFLDACTGSGVECTPFDNS
                     ILTKLKSDGSLPDLP"
     gene            complement(1252..2445)
                     /locus_tag="VFQ61_04790"
     CDS             complement(1252..2445)
                     /locus_tag="VFQ61_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953791.1"
                     /translation="MTIRSRRFAKRSAWAVAAGLLPLFQVASAMAAPCADLQNPVFVS
                     GSSAVQGFLKEVARELVAQDITVVYQSGGSCVGVNNVVGGTPLTGTATTWDAAGNVVN
                     CDLADDVADIGISDVYASTCGVNLPSGVQDFFGPVQAMTFVVPPKSTHASISAEAAYL
                     TFGLGDDGETPWSDYTKYIIRSASSGTQQMLSAAITVPAANWKATDANRKSGSGDVVT
                     AITAANATQAEADLTIGILSTDAVAKNSDKVKVLAYQHFGQSCGYLPDSSSSRFDKQN
                     VRDGHYYVWGPLHMLSPVKSGKPANANAKVVIDYLSGAADPAGFDLIQLEAKGGVVPA
                     CAMRVSRTTEVGPLSSFMPERSCECKFVREATGTAPAGCETCENNSDCPSSAPACNYG
                     YCEVQ"
     gene            complement(3380..5038)
                     /locus_tag="VFQ61_04795"
     CDS             complement(3380..5038)
                     /locus_tag="VFQ61_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953792.1"
                     /translation="MSAPSIAGNWGSGSPFVLPVELSDSELLSATRRLVGRSNQLLAL
                     LLGHLAELESRGLHRTRACSSLYTYCIYELRFSEDEAYRRVAAARLVRRFPALLDAVA
                     SGELHLTGLLMLGPHLTAENLVEVLARAKHRTKKEITSLVRQLDPLPDVPARIEPLGP
                     APARALSPTHGQFVESLCPVRELAPGERPREWAGNAAPTDVIEDVAFTAGAQFAEGAQ
                     FAEGAQLAERAAFTAGAQFTASAEGDSGELSNEFRLRECIAAGPERNSSAQFDSSAQF
                     DGSSHVIDARPAPRLDTSPVPARREAQRYRVEFTTSEEYVQLVEAAQTLLSHSTERIP
                     LAELQLRAMRALVTELEWKKRAATKRAAQPIVPASGAATRATRRPRAEQPAHPEQPAH
                     PEQPARATQSAPSVSVRNVVQPVPAETHEARDRATSAYEHPRQRESHQPEIPRQRESH
                     EPEIPRQRGRHVPAAVRRAVFERDQGRCAFIDGSGQRCRETHRLELHHLKAYALGGEH
                     AVDNLALRCQAHNTLVAEQDFGPDFIQRCRDSSRHESCKQQLGG"
     gene            complement(5642..>6184)
                     /locus_tag="VFQ61_04800"
     CDS             complement(5642..>6184)
                     /locus_tag="VFQ61_04800"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9953793.1"
                     /translation="ARAPSRRRIRIAFFSNEEPPFFKQAGMGSYVHAMNARRRGDPIK
                     GMLALETMGFYSHAPHSQRYPWPVGLLYPTRANFIAFVGDVGSRSLVRDAIGAFRELA
                     QFPSEGAALPTTFPGIDWSDHWAFRRAGYPAIMVTDTAPYRDPNYHRPTDSAERLDYR
                     SLARVTRGLEGVVRRLADAP"
BASE COUNT         1134 a         2054 c         1896 g         1100 t
ORIGIN      
        1 ctcccaacgt cgtttgaata ccagccgcgc ccgcctcacc aggaatcggt cctgattcag
       61 gagcgcaccg ccctgacgca gctgctcttc cgagtcttgg tgagattgat actccagttg
      121 tgcatacccg agtacgtcca ccagcggcgc gggtgctggg gcagccttct cggtcgctgt
      181 atacgtcttc tgcgcagccg tatgcgtctc actagaatcc ggttgttgcg cgatggagcc
      241 ggagggtgcc gcgcccgggg acgtcgcaat cccgactggg gccgacggag tcgatggggc
      301 cgacggagtc actggcgctg gcgtcggagc ggcgctcggt ggttcgcgaa ccgacggcgc
      361 agacgcgttc gagggcggcg cggccgtgtc ccctcccgcc gcgctcgtgt tgggagcagc
      421 ctgacccggc gcgacctcga tctccgcgtc atcaagcggt tcggtcggcg gagcaggctg
      481 cgccgccaag cgccccgcaa cgagatagct cgccagggcg gggccgagcg acaagcagca
      541 ataaagatgt ttccgaaacc gcaccacgac gttgcgtatc tccaaattca agaatgcccg
      601 agcggcgcac agcgccaccc tcgggcctca cagatccgat gaatcaggcc cgctctcctc
      661 gttgaggagt aacgggccac tctcgattca attcacatcc cctaccccgc tcgtcggatc
      721 accctcaagg cagatccggc agcgatccgt ccgacttcaa cttcgtgaga attgaattat
      781 cgaacggtgt gcactccacg cccgaaccgg tacaagcgtc caggaagtga gcatcctcga
      841 ccggtttgca gtcctcgtag caacccttgg agttcggctc gtcacagtca ctcagactgg
      901 tagagttgcc ggctccaccc tcccccgtgc tcgtttcacc cccttgcgcg cccgcgccac
      961 cctccaccgg cggctctccg ccgcttgcct taccgcccgt cgaactcttt cctccggtgt
     1021 tcgacgcgcc tcccttgctc gtcgtgccac cggtcccgcc ggtcgcactc tcgcctgcat
     1081 cagacgtgtc tccgccagag ctcgccccac ccttgctcgt cttgccgcca gagctctgcg
     1141 aaccgccttt gctcggcgtc ggattgtcgt cgtcatcccc gcaacccacg aaggcgcaca
     1201 gcgccgatga cacgaacagg accatgccca agccaaaccg attggaagac atcactgaac
     1261 ctcgcagtag ccgtaattgc aggccggcgc tgagctcgga caatcgctgt tgttctcaca
     1321 ggtctcacag cccgccggcg cggtaccggt agcttccctg acgaacttgc actcacagga
     1381 acgctccggc atgaagctcg acagcggccc cacctcggtc gtgcgcgaca cgcgcatggc
     1441 acaggccggc accaccccgc ccttggcctc cagctgaatc aagtcgaacc ccgccggatc
     1501 tgcggcgccc gacagataat caatcacgac cttggcgttg gcgttggccg gcttacccga
     1561 cttcaccggg ctcagcatgt gcagcgggcc ccacacgtaa tagtgcccgt cgcgtacgtt
     1621 ctgcttgtcg aatcgcgaac tcgatgagtc cggcaagtag ccgcagctct gcccgaagtg
     1681 ctggtacgcg agcaccttca ccttgtcgct gttcttggcg accgcgtcgg tcgacaagat
     1741 cccaatcgtc aagtccgcct ctgcttgcgt cgcgttcgcg gccgtgatcg cagtgaccac
     1801 atccccgctg cccgacttgc gattggcgtc cgtggccttc cagttggcgg ccggaaccgt
     1861 gatcgcagcc gacaacatct gttgcgtgcc actgctcgcg ctgcggatga tgtacttcgt
     1921 gtagtcgctc cacggcgtct ccccgtcgtc gccgagccca aacgtcaagt atgccgcctc
     1981 cgcgctgatc gaggcatggg tcgacttggg tgggaccacg aaggtcatcg cctgaaccgg
     2041 cccgaagaag tcctgcaccc cactcggcag attcacgccg cacgtcgagg catacacgtc
     2101 ggagatcccg atatccgcca catcatcggc caagtcacag ttgaccacgt tacccgccgc
     2161 gtcccacgtg gtggccgtgc cggtcagagg ggttcctccc accacgttat tgacgccgac
     2221 gcacgagcca ccggattgat agacgacggt gatatcctgc gccaccagct cgcgcgcgac
     2281 ctccttgagg aagccctgca ccgcggaaga accgctcacg aacaccggat tctgtaggtc
     2341 cgcgcagggc gccgccatcg cactcgccac ctgaaacaga ggcagtaggc ctgccgccac
     2401 cgcccaagcg gagcgcttgg cgaaacggcg cgatcgaata gtcatcgctg caatcctttc
     2461 gaaactcggc gcttccaact tcaaagcgcg aggcactgtg taagagaact gcagggcgct
     2521 tgcgcgaccg tcccgttata tctaggtgac gcgcgctgcg agaccgtcac ggagcacgac
     2581 gtcaccccta tggattaaca gttcgccggg ctcccaattc gatatttatc ggccccctgc
     2641 tttgtgctcc tttatccagg cgccgtcaca ccaaggcctt ggattcgtca tcttcgcgtg
     2701 gtttgggatc cgtcgtgaca acgtgcgcgc agcactcgaa acgcctcgtt ggcaccggcc
     2761 ctgttcccat cctccgtcaa aacaccgcgc tcggcccacc tcgtaaagcc tgcgcgcacg
     2821 ctgtgtttac ttggggcacg ccgatgagta aatcatcgcg agccggccga gaccgcagcg
     2881 acgcaagcga gaggagagag caccggaagc ccatagacta aggagcgcag cggcgagcct
     2941 tgccaggcga aactctgtca gacgagagag tgcagcaaga ggatcgacca gagttccatc
     3001 cctcggcgaa gccgctgaag aattgtgccc acaagcacgg atcgagcggc tcgccgcgtt
     3061 ggcttgcacg ggtggaatgc ttttggagtt gccgaggacg tagcacaggc gtgacggaca
     3121 gccgcggcga ctgaagcgag tgctcagaaa agcgcactcc gtccctctgc cggcaagcgg
     3181 tgttttggca cgaacaactc ccactatgct ggacgagcga ctcgtagtac cccgacgtct
     3241 gcttgcaacg agtgtattca cgagcaagcc acctccagaa gctcatcagc gtgctctgaa
     3301 tggaggatcc agctgcccgc cgcgtgcggg ctggctccgt caggcaagct gaagcctgtg
     3361 ccttcgccgt accgcggtcc tagcctccta actgctgctt gcaagactcg tgtcgactcg
     3421 agtcgcgaca ccgctggatg aagtcggggc caaagtcctg ctcagcaacc agggtattgt
     3481 gcgcctggca gcgcagcgcg agattgtcca ccgcgtgctc gccacccaga gcatacgctt
     3541 tcaggtggtg gagctcgagc cgatgcgttt ctcggcaacg ctgccctgaa ccatcgatga
     3601 acgcgcagcg cccctggtcg cgctcgaaga cagcacgccg aaccgccgca ggcacatgtc
     3661 gcccgcgctg gcgcgggatc tcgggttcat ggctttcgcg ctggcgcggg atctcgggtt
     3721 gatggctttc gcgctggcgc gggtgctcat aggcggaggt ggcgcggtct cgagcctcgt
     3781 gagtttcggc aggaacgggt tggaccacgt tccgcaccga aaccgagggc gcactctgcg
     3841 ttgctcgggc tggttgttct ggatgcgctg gttgttctgg atgcgctggt tgttctgctc
     3901 gaggccgccg cgtcgctctt gtggcagctc cgcttgccgg aacgatgggc tgtgccgccc
     3961 gcttcgttgc ggcgcgcttc ttccattcga gctcagtcac gagcgcgcgc atcgcgcgga
     4021 gctggagctc agcgaggggg atgcgctcgg tagagtgaga gagtagggtc tgggccgcct
     4081 cgacgagctg gacgtattcc tcgctagtgg tgaactcgac gcgatagcgt tgagcttccc
     4141 gccgcgccgg cacgggcgac gtatccaggc gcggtgccgg ccgcgcatca atgacgtgcg
     4201 aggaaccgtc gaactgagcg gagctgtcga actgagcgga gctgttgcgt tcgggtcccg
     4261 cggcgatgca ctctctcagc cggaactcat tggagagttc gcctgagtct ccctccgcgc
     4321 tcgcggtgaa ctgtgcaccc gcggtgaacg ctgcgcgctc agcaagctgc gcgccctcag
     4381 caaactgtgc gccctcagca aactgtgcgc ccgcggtgaa cgctacatcc tcgatcacgt
     4441 cggttggcgc ggcgttaccc gcccactcgc gaggccgttc gcctggcgcc agctcgcgaa
     4501 ctggacacag cgactcgacg aactgtccat gggtcggcga aagcgcacgc gctggcgcgg
     4561 gcccgagcgg ctcgatgcga gccggaacgt ctggcagcgg gtcgagttgc cggactaggc
     4621 tcgtgatctc tttcttcgtc cgatgcttcg cccgggccaa tacttcgacg agattctcgg
     4681 cggtgaggtg cgggccgagc atcaacaagc cagtgaggtg gagctcgccg gacgcgaccg
     4741 cgtcgagcaa agccgggaag cgccgaacca agcgcgcggc cgctacacgg cggtaggctt
     4801 cgtcctccga aaatcggagc tcatagatac agtacgtata gaggctcgaa caggcgcgag
     4861 tgcggtgaag cccgcgcgac tccagctcgg cgaggtgacc gagcagcagc gccaacagct
     4921 gattggaccg acctacgagg cgtcgtgttg cactgagtaa ctcgctgtca gacagctcga
     4981 ccggcaagac gaacggagac cctgaacccc aattcccggc gatactcggc gcgctcatga
     5041 actccatctt acaccctgat tttcggcgct cgacattcgc gctctcgtgc gtcttcgcgc
     5101 gcagaaacga cgtttgagcc aactgcccgc acgaaagcgg cacagcgaga cacgcgctcg
     5161 cggcaaacgc taaagagccg aactcacgcc ccgaactgca accgacgctc gccaatcgtg
     5221 tcaagcatcg aatgcgagtt cccgacgaag tctgtaagac cgacgcggct gctcgtcagc
     5281 cctgcaatcc ggtccggcgc gcttggcgac cgcagagggc gatccagata agtcccaact
     5341 atctgcctgt atctggtgat gcgccgcgac cgtgccactc cgttagtctg tcctcggtag
     5401 tctttcgctg gtctggaact cagtgcagag gacgatccct gagggcacgc gcgaggccaa
     5461 cgatgactag ctctgattgg cctgcatgga gaactgcaca attcgagagc gttcaccacg
     5521 taacgagttc accgcacaaa atcgcgcggc ggacgagggc gaagacgcgc tcgcaaaaca
     5581 acgcacggcg acgcgaatca atcatccgag agcggacgcc gcggcgtggg tgtgcgattc
     5641 atcacggggc gtcagccagc ctccgcacca ctccctcgag gccccgggtt acccgcgcga
     5701 gggagcggta atcgagccgc tctgcgctgt cagtggggcg atgatagttg ggatcgcgat
     5761 agggagctgt atcggtgacc atgatcgcgg gatagccggc gcgcctgaag gcccagtgat
     5821 cggaccaatc aatgcccgga aatgtggtgg gcaacgccgc gccttcagac gggaactgcg
     5881 cgagctctcg aaacgctccg atcgcgtcgc ggacgagcga acggcttccc acgtcgccga
     5941 cgaatgcgat gaagttggcc cgcgtgggat acagcagccc aactggccac ggatagcgct
     6001 gcgagtgggg tgcgtgcgaa tagaacccca tggtctcgag cgcgagcatg cccttgattg
     6061 ggtccccgcg ccgccgcgcg ttcatggcgt gcacgtagga acccataccc gcttgcttga
     6121 agaagggggg ctcctcgttg gagaagaacg cgattcgaat gcgacgtcgg ctcggcgcgc
     6181 gcgc
//
LOCUS       DASTKU010000135         4079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1140529, whole genome shotgun sequence.
ACCESSION   DASTKU010000135 DASTKU010000000
VERSION     DASTKU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140529"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..3032)
                     /locus_tag="VFQ61_04805"
     CDS             complement(99..3032)
                     /locus_tag="VFQ61_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953794.1"
                     /translation="MMERFSGWYLLVIVGAAGCSGASPPSESEAAESETSQSQSERLD
                     GLHVEAPVEVSRAVTALRSQFRQRGEAFTARGRQHEIEIGRGVTRLRRIPSSQELRST
                     AEARVGRVSRSKALLPPAVEIKTRSIARGSRESMTGAAAWAVARNGDALRRFERGEEH
                     WTNLAEGAEVAYHFAERPAGAGDLVVSVDVNTGSSSPSVRSDARGLHFQMADGHRFFF
                     EHATWVDASGKRVAVPARWKADPEHGAVELVVPAKLIEQSQYPAILDPLVGPDLGTDD
                     PVLTQSSAGTEPDVASDGSNFMAVFGDLQRIRAVRVDASGHVLDANWLDLGEDGKLQF
                     EPAITFGGGHYLVAWWEDDGTQVTIKGRVLNPDGSLVGTSNVALSSDTGFDAALAWNG
                     SSFMASWIGYGDTPGIRVALVDSSGQVVSGSEHLVSTNIRDSHPALAVGTTGALVAWQ
                     DESASDFTGRLRAARLGLDGSVLDPEGFRLSSVESDENQASVAAAGDRFLVAWHRAGG
                     GQPGSIRGTVIDSAGIAAPDFAISRSSGEASYPSVGFNGEAFAVAWKDERETPGIRGT
                     LVTPEGSVSGAEDSLLSNVPATPGGYFDNTGIAWNGSNFLLVFQGMRSTGISSIVGIE
                     GSFLGADLSVRPGPLGLSQLRAGEFAPQVVWDGRNYVVSWTDQRLGNFELSSSRAVRI
                     SSAGQVLDPPGIPLSDDAPGLGWTASNADRRTLVTLSEPSGSAQYRFLNANGQLSPKR
                     PLADGVTGPVVAAGNGTSYLAVMTNAHEDYMYDLTGRVIRANGSAGPEFSIEPLTNGS
                     GGVIATGDGYLVSSWTDFGKLFPVSANGVVGTPISLSLASTSLASATNGRRTLVAWTP
                     GIGELPFAAQARFMVDGAFRGPTLEIAPTTAGSLAALAWDGRSYWAVWTVGDDSPRPF
                     IRSISANGVLGPVSPVFEGECRGPALASNGRRQLLLSCYAYTNQYRLVTVSTRLIDTS
                     SASVQ"
     gene            complement(3291..3815)
                     /locus_tag="VFQ61_04810"
     CDS             complement(3291..3815)
                     /locus_tag="VFQ61_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953795.1"
                     /translation="MTNRRLRVAAAIVGLCAGTLQVLAWWLSTQVESLYAEERLWLIG
                     EYSEPWHSLARVVAIGVSTGVFLLPFRLDRRDILVKALALVLGLAISFELATAVHFNI
                     ISGELSVDTLIWSRSRLRLDAGETGWCVKADLSHPWVWRIDGQAVYPRLLPLPQNTGP
                     ILDRLRYPSCPVMK"
     gene            complement(3812..>4079)
                     /locus_tag="VFQ61_04815"
     CDS             complement(3812..>4079)
                     /locus_tag="VFQ61_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953796.1"
                     /translation="ASMALRTSAARSGSEHGRPSFVELRGEHPIRITEEFVHKLRRFH
                     SGRFSVTRATRESGVPLPSRTLTSRSGPHYGRLMLGAAQLTIEP"
BASE COUNT          781 a         1341 c         1222 g          735 t
ORIGIN      
        1 agctcactcg ccgaggccaa cacgagcggg cgcacgcagc tcccgctcgt gtcttggaca
       61 ttcaatctcg ttccaaagag gtgcgcgtta ggcgctcgtc actgaacgct cgcactcgag
      121 gtgtcaatga gtcgcgtgct caccgttacc aggcgatatt ggttggtgta cgcgtagcag
      181 gagagcaaga gttgtcgccg gccgttgctc gcgagggcgg ggccacggca ctcgccctcg
      241 aatacagggc tcaccgggcc gagcacgcca ttcgccgaaa tcgaacggat gaatggacgc
      301 gggctatcat cgcccaccgt ccaaaccgcc cagtagctgc gcccgtccca agccaatgcc
      361 gccagagatc cggcggtcgt gggtgcgatt tccaaggttg gccctcggaa ggcgccatcg
      421 accatgaagc gcgcttgtgc ggcaaacggc aactctccga tgccaggggt ccacgctacg
      481 agcgtgcgcc gaccattggt ggccgacgcg agggaagtcg aggcgagcga caacgagatc
      541 ggagttccca cgacgccatt ggcgctgacg gggaagagct ttccaaaatc agtccaggaa
      601 gagaccagat agccgtcacc cgtggcaatg acgccgcctg aaccattcgt gagaggctcg
      661 attgaaaact ccggtcccgc gctgccgttg gctcgaatca cgcgccccgt cagatcatac
      721 atgtagtctt cgtgcgcgtt ggtcatcacg gccaagtagg aggtgccatt gccggccgcc
      781 acgacgggac cggtcactcc gtctgccaag gggcgtttgg ggctgagctg cccgttggcg
      841 ttcaggaatc ggtactgcgc ggagccgctc ggctccgaca acgtaactaa cgtcctgcga
      901 tccgcgttgg aagccgtcca acctaggccg ggtgcatcgt cggacagcgg gatgccgggc
      961 ggatcgagca cctgtccggc gctgctgatg cgtacggctc gagagctcga gagttcgaag
     1021 ttgccgagtc gctgatctgt ccagcttacg acgtagttgc ggccatccca caccacttgc
     1081 ggcgcgaact cgccggcccg caactgcgaa aggccgagag ggcccggacg aacactgagg
     1141 tcggctccca ggaagctgcc ttcaatgccg acgatgctgc taattcccgt cgagcgcatg
     1201 ccctgaaaaa cgagcaggaa gttcgaacca ttccaggcga tcccggtgtt gtcgaagtag
     1261 ccgccgggcg tggcgggcac gttcgagagc aggctatctt ctgcgccact caccgagcct
     1321 tccggcgtga cgagtgtgcc ccgtatacct ggagtctcgc gttcgtcttt ccaggccact
     1381 gcgaacgcct ctccattgaa tccgacgctg ggataagagg cttccccgct cgatcgtgag
     1441 atggcgaaat ccggggctgc gatccccgcg ctatcgatca ctgtcccccg aatgctaccg
     1501 ggctggccgc ctccggcgcg gtgccaagcg accaagaagc gatcccccgc ggcggcgaca
     1561 ctcgcctgat tctcgtcgct ctctacgctc gagaggcgga atccttctgg atccagaaca
     1621 ctgccgtcca agcccaatcg cgccgcgcgg agccgtcccg tgaagtcgct cgcgctctcg
     1681 tcctgccaag ctacgagtgc accggttgtg cccaccgcca aagcgggatg tgagtctcgg
     1741 atattggtcg atacgaggtg ctcggatccg gatacgactt gtccgctcga gtcgaccaag
     1801 gccactcgga tgccgggtgt atcgccgtaa ccaatccaac ttgccatgaa cgacgagcca
     1861 ttccaggcca gcgctgcatc gaaccccgta tcggacgaga gggcgacgtt cgatgtcccg
     1921 accaagctgc cgtcggggtt caagacgcgg cccttgatgg tgacctgagt tccatcgtcc
     1981 tcccaccaag cgacgaggta gtgaccgccc ccgaacgtga tggcaggttc gaactggagc
     2041 ttgccgtctt caccgaggtc gagccaattg gcgtccaata catgcccgct cgcgtcgacc
     2101 cgtacggccc gaattcgctg caagtctccg aataccgcca tgaagttact gccgtccgac
     2161 gcgacgtcgg gctccgtgcc ggcggaggat tgagtcagca ccggatcatc ggtcccaaga
     2221 tcgggcccca ccaaggggtc gagaatcgcg ggatattgac tctgctcgat cagcttcgcc
     2281 ggtacgacga gctccaccgc tccgtgctcc ggatcggctt tccaacgcgc tggcacggcc
     2341 acgcgcttac ccgatgcgtc tacccaagtg gcgtgctcga agaagaagcg gtgcccatcc
     2401 gccatctgaa agtgcagacc gcgggcgtcg ctgcgtacgg agggtgagct cgagccggta
     2461 tttacgtcga cggagacgac cagatctcca gcccctgccg gacgctccgc gaagtggtag
     2521 gcgacctccg cgccttccgc caagttcgtc caatgctcct cgccgcgttc gaagcggcgc
     2581 aatgcatccc cattgcgagc caccgcccaa gctgccgctc ccgtcatcga ctcgcggctg
     2641 ccccgtgcga tggatcgcgt ctttatttcg acggccggcg gaaggagtgc ctttgatctc
     2701 gacactctgc cgacgcgagc ctccgctgtc gatcgcagct cttgcgaact aggaatgcga
     2761 cgcagtcgcg tgacgccgcg cccaatctcg atttcgtgtt ggcggcctcg cgccgtaaag
     2821 gcttcaccgc gctgccgaaa ctgactccgg agggcggtca ccgctcgact cacctcgacc
     2881 ggagcttcta cgtgcagccc atcgagccgc tcgctttgcg attgactcgt ttcgctctcg
     2941 gctgcttcgc tctcgcttgg gggactggca cccgagcagc ctgccgctcc tacgatcacc
     3001 aataaatacc acccgctgaa acgttccatc attgcaacat ctccccaaaa agtgacggtc
     3061 gtgacggaca gcgtcttcgc ggagtttgcc taaatcacac cttccaaccc gccgaagccg
     3121 ctcactgtat atagcgcttg cggatagaaa ggggaagtgt cgcgagcagg tgcgtgtcag
     3181 taacaactgt gccgccgagc aacactcgcc agtcctccgt tcagggtggg ctctggtcta
     3241 gttccgcgaa actaggattc gcactcgatt ggattcagcg cttcgagcgc ttacttcatg
     3301 acggggcagc tgggatatcg caggcgatcg agaatcgggc ccgtattctg gggtagcggg
     3361 agcagccttg gatacaccgc ttgtccgtcg atgcgccaaa cccagggatg gctcaggtcg
     3421 gccttgacgc accagcccgt ttccccagca tccaagcgta gccgcgatcg actccaaatc
     3481 agagtgtcca cggagagctc cccggagatg atattgaaat ggaccgcggt cgcgagctcg
     3541 aacgaaatcg ccaatccgag cacgagcgcg agcgctttga ccaggatgtc tctccggtcg
     3601 agccggaacg ggagcagaaa cacccctgtg ctcacgccga tcgcgaccac tcgagccaag
     3661 gaatgccagg gctccgagta ctcaccgatc aaccagagcc gctcttccgc atacaggctc
     3721 tccacttgcg tgctcagcca ccacgcgaga acctggagtg tgcctgcgca gagacccacg
     3781 atcgccgccg ccacgcgaag tcttcggttc gtcatggttc aatcgtgagc tgggcggccc
     3841 cgagcatgag ccgaccgtaa tgcgggccag aacgcgacgt caacgtgcgg ctcggaagcg
     3901 gaacccccga ctcgcgtgtt gctctcgtaa ccgaaaaccg acccgagtga aatcggcgca
     3961 acttatgaac gaactcttcg gtgatccgaa tgggatgctc cccgcgtagt tcgacgaatg
     4021 acggccggcc gtgctcgctg ccgctgcgcg cggccgaggt cctcaacgcc atgctcgct
//
LOCUS       DASTKU010000136         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_26186,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000136 DASTKU010000000
VERSION     DASTKU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26186"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..1250)
                     /locus_tag="VFQ61_04820"
     CDS             complement(393..1250)
                     /locus_tag="VFQ61_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953797.1"
                     /translation="MASWDHEGIVELFRSDPGLAVELLRGPLGLELPPFAVARVETGA
                     LTQLNPAELRADLVISLSNNGEHPVLAVIVEAQRDQDDDKLFSWPAYVGSLRHRLRCP
                     VCVLVVTQSERVANWASRTITVGPGCSLSPLVLRPSSVPVISLAEEARQAPELAVLSA
                     MAHGGGAVETAVQVALAAATAAHELGRDRFLLYFGLIRAALSEAARKAFQMLPQGAQF
                     FDESQKQSFDRGRAAEKAAAVLEVLDARGLAVTAAQRERILSTKELETLTHWVRRAAT
                     IASADALFE"
     gene            1269..2075
                     /locus_tag="VFQ61_04825"
     CDS             1269..2075
                     /locus_tag="VFQ61_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HET9953798.1"
                     /translation="MGGNFNSHAYDSLGNLQQKRLSRTLDGTITAQTIEHTPHCTRPH
                     LLATIGANTYTYDAKGNLTGRAGPDVPGGEQLIEYTLFDLPKKVITGPSATGQTTRFE
                     YSANEERLVRRDADAKRHFVPELYQRLISSNGATVEERFALYAGDRPLGEIVRKNDTD
                     EVLYFHTDRLGTVDTISTSTSDVVQQGFDPFGAPLASPGPEVTRAGFTGHQHDRDLGL
                     IDMRGRVYDPFASRFATPDPVTQAPYWSQGLNRYAYVFNDPSIIRIRAGL"
     gene            2153..2893
                     /locus_tag="VFQ61_04830"
     CDS             2153..2893
                     /locus_tag="VFQ61_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953799.1"
                     /translation="MANLSPVGVITAGGFNIGLTLGLGLGSGQAASGPQAVQTNAQQA
                     SNTRPAAQNSMSATGQREPTVVWGKDGFSSNLKPPAKDTSGYIACYPACATDPNATFP
                     NAESAGAYSTSYEIVGSGILVAEGVIGLGRWALGRLGFGAARGVGEVIRLPQSLGAAA
                     KEIFVRGGGRLVSRQITNVEKIAEGRGIREVGRLVEQYGGKAANWRKMKGIADVERAG
                     GEIGRAEVHWYQGHGVGRVEFKVKAWLD"
     gene            2881..3114
                     /locus_tag="VFQ61_04835"
     CDS             2881..3114
                     /locus_tag="VFQ61_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953800.1"
                     /translation="MARLMRVRCIDNRPFLHEDGGQLVQEARSGELVSLTVGKIYSVL
                     SIEKGWYRVVDDTDEDYLYPPSLFEVVQPDGRS"
     gene            complement(3439..>4007)
                     /locus_tag="VFQ61_04840"
     CDS             complement(3439..>4007)
                     /locus_tag="VFQ61_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953801.1"
                     /translation="VLDKVLYTLCNPVVEHLVDRVTDWPGANSYRAMLSGEAIEVKRP
                     RDFFRADGTTPEVARLELVRPPGFEECDLAHWREHLSGLVTAIEERTRAERHRSGAKL
                     LGRKAVLRASAFDTPQSSEPRRNLRPAVACRDEATRVHVLTQLLAFRRAHEAARQRVA
                     AGELEAMFPYGTYRMRLMGHRCASVPLS"
BASE COUNT          772 a         1287 c         1275 g          673 t
ORIGIN      
        1 ctcgcgcggg gcacacatcg cgactgcacc gaaagcgcag cgcgtgctag gcggaaacca
       61 cggacgccga gcgaactacg agctggccgc cagtggcttg gggtggcgca gctgccgcgc
      121 gtggacgcga cgtcctctcc gcgctgcagg accctcggcc acgcgcagcg ccggcttcgg
      181 cgcgagcatg gccttgaggg cctcgggccg cgcgcagcgg cagcgagcac gggacggcgc
      241 tataacgggc tctgcagcgg gggagcacca ctctccgatg cttcagaata ctcgcgccgc
      301 gcggcgagct tgccgccgac ctaacttgcg gcccaaagaa cttaacgtcg ttcgggatca
      361 cccgggatca cccgcgacaa cggtagcgag gactactcga acagcgcgtc agcggaggcg
      421 atagtggcgg cgcggcggac ccaatgggta agtgtttcga gctccttggt gctcaagatt
      481 cgctcgcgct gcgcggcagt cacggcgagg ccacgagcgt cgagcacctc cagaaccgcg
      541 gcagctttct ccgcggcacg gccgcgatcg aaggattgct tttgcgactc atcgaagaac
      601 tgcgcgcctt gcgggagcat ctggaatgcc tttctggcgg cctcgctgag cgccgcgcga
      661 atcaagccga agtatagcag gaagcggtca cggccaagct cgtgggccgc ggttgctgca
      721 gccagagcta cttggacggc tgtctcaacg gccccgccgc cgtgcgccat cgccgaaagt
      781 actgcgagct ccggcgcctg ccgggcctcc tcagccaggc tgatgaccgg gaccgaactt
      841 ggtctgagca cgagcggcga gagcgaacag cccggcccaa ccgtgatggt gcgtgaggcc
      901 cagttagcaa cgcgctcgct ttgcgtgacc acgagcacgc acaccggaca gcgcaggcgg
      961 tggcggagcg accccacata cgccggccag gagaacagct tgtcatcatc ctggtcacgc
     1021 tgcgcctcca cgatgacagc cagcaccgga tgttcaccgt tgttgctcag ggagataacg
     1081 aggtcggctc gcaattcggc cggattgagt tgggtcagcg cgccagtctc cacccgcgcc
     1141 acagcgaagg gcggcagctc tagcccgagc gggccccgca agagctcgac ggccaagcct
     1201 gggtcgctcc tgaacaactc gacgatgccc tcgtggtccc aagaagccat cggcagcaag
     1261 ctacacaagt gggaggcaac ttcaactcac acgcctacga ttcgctcggc aaccttcagc
     1321 aaaagcgact cagccggaca ctcgacggga caatcacggc tcagacgatc gagcacactc
     1381 cccactgcac acgccctcac ctgctcgcga ccattggagc caatacctat acttacgatg
     1441 ccaagggcaa cctcacgggg cgggcgggcc cggacgtccc cggcggtgaa cagctcattg
     1501 agtacaccct gttcgacttg ccgaagaagg tcatcacggg ccccagcgca acgggccaga
     1561 ccactcgctt cgaatactcc gcgaacgagg agcgcctcgt gcggcgcgac gcagacgcca
     1621 agcggcattt cgtgccggag ctctatcaaa ggctcatcag ctccaacggc gccaccgtcg
     1681 aggagcggtt tgcgctatat gctggggacc gcccattggg cgagattgtc cgcaagaacg
     1741 ataccgacga ggttctctac ttccacaccg accggcttgg caccgtggac accatcagca
     1801 ccagcacgag cgacgtcgtc cagcaagggt tcgacccctt cggcgctccg ctcgcgtcgc
     1861 ccgggcccga ggtcacgcgg gcgggcttca ccgggcacca gcatgatcgc gacctgggtc
     1921 tgatcgatat gcgcggccgt gtgtacgatc cgtttgcttc gcgctttgct acgccggatc
     1981 ccgtcactca ggcgccctac tggtctcaag ggctcaatcg gtacgcttac gtctttaatg
     2041 acccgtcaat aatacggatc cgagcgggtt tatgagcgat gggaattcgg gagcagccgg
     2101 tttatccgct tcggcgccct gggcgggcgc ctacgcgagc gcggcagcca ccatggcaaa
     2161 cttgtctccc gtaggcgtta tcaccgccgg cggattcaat atcggtctca ctctaggttt
     2221 ggggcttggc tcggggcagg ccgcgtcggg gccgcaagct gttcagacca acgcgcagca
     2281 ggcgtcgaat acgcggccgg cggcgcagaa ttcgatgagt gcgacggggc agcgcgagcc
     2341 gacggtcgtc tgggggaagg acggattcag cagtaacctc aagccaccgg cgaaggacac
     2401 gagtgggtat atcgcgtgtt acccggcgtg tgccacagat ccgaatgcga cgtttccgaa
     2461 cgcggagtcc gcgggcgcat acagcacaag ttacgagatc gttgggagcg ggatacttgt
     2521 tgcggaaggc gtgattggtc tcggccgttg ggcgctgggg cggctggggt tcggtgccgc
     2581 aaggggggta ggcgaagtca ttcgtctgcc tcagtcgtta ggagcagccg caaaggagat
     2641 ctttgtgcga ggaggcggca ggctggtaag ccgccagatc acgaacgttg agaagatagc
     2701 ggaggggcgt ggcatccgtg aagttggcag gctcgttgag caatatggtg gaaaggccgc
     2761 caactggaga aagatgaaag ggattgccga cgttgagcgg gctggtggtg agattgggag
     2821 ggcggaagtc cactggtatc agggccacgg cgtgggccgt gtcgaattta aggtgaaagc
     2881 atggctcgat taatgcgagt gcgctgcatc gacaaccgtc cgtttctcca cgaagacggc
     2941 gggcagctcg tacaagaggc ccgctccgga gagttagtca gcctcacagt aggaaagatt
     3001 tatagcgtgc tcagcattga gaaaggttgg taccgcgtgg tggacgacac cgacgaagac
     3061 tacttatatc cccctagcct cttcgaagtc gttcaacctg atgggcgctc gtgactcgct
     3121 ccgacgtcaa gctaaattca tgccgcaagg ggagtagcga gcggcgcgat ggccgggagt
     3181 gatcccgtac gaggttactt tcaacgtcag cgagaacgac ccgtccagtg atcccgtacg
     3241 aggttacttt caacgtcagc gagaacgacc cgtccgaacc ctgcccctca agcgctccgg
     3301 cgcgcaaatg tcgctccaaa ctgcgcgcca ccgctgcccc tcgcagacgc acctttcccc
     3361 tcccgcatgg ctcccctcca caagccgcag gtcccccttg gccgcgcgcc tcgcggccaa
     3421 atccaggcgc cgactctgct agcttaacgg caccgacgcg cagcgatggc ccatcagacg
     3481 catccgatac gtcccgtacg gaaacatcgc ttcgagttcg cctgccgcga ctcgctgccg
     3541 cgcggcttcg tgcgcgcgac ggaatgccaa gagttgcgtg agcacgtgca cccgagttgc
     3601 ctcgtctcta cacgccaccg cgggccgcaa gttgcgtcgt ggctcagagc tctgcggcgt
     3661 gtcgaacgcc gatgcgcgaa gtaccgcttt ccgacccagg agcttggctc cgctccgatg
     3721 gcgctccgca cgagtgcgtt cctcaatcgc ggtgaccagt ccgctcaggt gctcccgcca
     3781 atgcgccaag tcgcactctt caaaaccagg cggtctcacg agctcgagtc gcgccacttc
     3841 cggcgtggtc ccgtccgctc ggaagaagtc ccgcgggcgc ttcacttcaa tggcctctcc
     3901 gctgagcatc gctcggtagg agttggctcc cggccaatcc gtcacgcggt ctaccaggtg
     3961 ctcaacgacg gggttacaca acgtatacag caccttgtcg agcacgt
//
LOCUS       DASTKU010000137         3813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1810130, whole genome shotgun sequence.
ACCESSION   DASTKU010000137 DASTKU010000000
VERSION     DASTKU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810130"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFQ61_04845"
     CDS             complement(<1..326)
                     /locus_tag="VFQ61_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953802.1"
                     /translation="MNLPIHAARSRSSDIAIQSVYEVTKLLAASPRPLERLGEVLSLL
                     SHFLELRNAGVALQAMTSQPEVVGLHAMQTDAALRWSALLDLALQRTRSTQMPWVVES
                     ERGEYG"
     gene            643..1548
                     /locus_tag="VFQ61_04850"
     CDS             643..1548
                     /locus_tag="VFQ61_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET9953803.1"
                     /translation="MANVTFVSPRMPRDVTVYAVAGDRGTILALAKAHKIPIPFDCQD
                     GECGSCLVQIQHVSAARPYGIALTEKEKELLKQLGKITREEIMDAEVNDLPPRYRLAC
                     QCFVRNEDIIVSFEGDATLPEQGPHLSIAAGVYTGGVVIDTLDQFLNFAVQVEEDAAV
                     HFETLANKMEECGNADVTALFRQLAGYSRLHLAEAKARSQAHDVEVKLPPKSAWPDNE
                     TPERTALWAGDPALSRLDALKAALQGERRGYEFYYAVAGTTADPAIRKVAREFVKEEA
                     QHVDTLKLWIAKEEAALREKARAAG"
     gene            complement(1599..2588)
                     /locus_tag="VFQ61_04855"
     CDS             complement(1599..2588)
                     /locus_tag="VFQ61_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953804.1"
                     /translation="MTAAREAGASEPVRLQWVRLEGAGSCIDASSLSERVRRRLGNDP
                     FQPQAQRSIEGMVRREAGTWRAQIAVRAHATDTEPAVRELKSTSQGCESLGDAVVLAV
                     ALAIDPEAALAASASEASPPATQAPANAAPAPTPVSREVPARAPDVPREAPPRTSPSG
                     RVAAGAVAQAGLLPHVSFGGGLLAAAALSSTFDVGVQARVFPAVRGSNGEFNFQIGAA
                     AGALQLCAGLPASTAMLIHVCAGPLLGLMHAALLSGERSQPGERAWLGAELGADLDLR
                     LTRWSALLLGARGVLPITRYRFAIEGSGGTIFRQAALAGMVQAALQLRFGAND"
     gene            complement(2633..3442)
                     /locus_tag="VFQ61_04860"
     CDS             complement(2633..3442)
                     /locus_tag="VFQ61_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953805.1"
                     /translation="MKTSTLALSSFSVLACSMDWSLARENSAGAGGSVDPGLMSAALL
                     CDRIPPLASEPIIDGKLDSGLSLYSWIDETTSDSNAGVRADLSLAYRSDGLYFFVAVT
                     DPTRDPAPLDALAYCGDGVELYVDDDGTIRSPPAYDRPGTMQFVVAAPSSETAAVRRG
                     QRFVSPGSGDGVDLGAWASTRFTAAPTPSGYAVEAFIVSNDLDLPSWALASGAEIGWN
                     LSLNIGGPEEPGLDVCTTRTRQVHFRFANSGTCTAPYCNASALCATALQSP"
     gene            complement(3439..>3813)
                     /locus_tag="VFQ61_04865"
     CDS             complement(3439..>3813)
                     /locus_tag="VFQ61_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953806.1"
                     /translation="APVSLEGSPIASAEPPTRPHGRGASVISAPQPSSEEDTLSGETQ
                     LLNEAHRALSTDPNRALAIAREHAKRYPRGQLAAERELILVQALVKLGRRREAEAKGR
                     GLRKAAPNSIYEERLDKVLGAP"
BASE COUNT          705 a         1207 c         1239 g          662 t
ORIGIN      
        1 aatccgtatt ccccgcgctc actctcgacg acccaaggca tctgcgtcga gcgggtgcgc
       61 tgcagcgcga gatcgagcag cgcggaccaa cgcagggcag catcggtttg catcgcgtgc
      121 agaccaacga cctccggttg agaggtcatc gcctgaaggg cgacgccagc attccgcagc
      181 tcgagaaaat gagagagcag gctcaggact tcgcccagcc gttccaaggg acgcggcgag
      241 gccgccaaca gcttcgtcac ctcatagacg ctctgaatgg caatgtccga actccgtgag
      301 cgcgcagcat gaatcggcag gttcatggca cttgcctccg gtatctccgc ttgggagagc
      361 gccagattgg cactctcttc gactgcaatt gtcggcggct caggttgcgg gaactttcct
      421 gacgaatgtc aggaggcttc cggcgtattt cgcgtgtcta atgcgacgcg ggtgtttagc
      481 gcaattgtcg cgattgtgag gttccctgca ggcccttcgg ctaccacctg cgatgcgatc
      541 tggatcaccc gccgattcgg cggtttggtg aacagcggag tggttgtcgc tcgtggcacg
      601 cgatctgcac agcgcgcggc cgactggagc tcgatttcaa caatggccaa cgtaacgttc
      661 gtttctcctc gtatgccgcg tgacgtcact gtgtacgcgg tggcaggcga ccgggggacc
      721 atcctggccc tcgccaaggc gcacaagatt cctatcccat tcgactgtca agatggagag
      781 tgtggatctt gcctggttca gatccagcac gtctccgccg cacggcctta cggtatcgca
      841 ctaaccgaga aggagaagga gctgctcaaa cagctcggaa agatcacgcg cgaggagatc
      901 atggacgcgg aggtgaacga cttgccgccg cgctaccggc tagcatgtca gtgtttcgtc
      961 cgaaacgagg acatcatcgt atccttcgaa ggagatgcga cactacccga acagggacct
     1021 cacttgtcga tcgcagcggg cgtctacacg gggggcgtcg tgatcgatac gctcgaccag
     1081 ttcttgaact tcgccgttca ggtggaagag gatgccgccg tgcacttcga gacacttgcc
     1141 aacaaaatgg aggagtgcgg taacgcagac gtgaccgcgc tgttccgtca gctcgccgga
     1201 tattctcgtc tgcatctcgc ggaagccaaa gcgcgctcgc aggcgcatga tgtcgaagtc
     1261 aagcttccgc ccaagtccgc ctggcccgac aacgagactc cggagcgtac ggcgttgtgg
     1321 gcaggggatc cggctctctc ccgcttggat gcgctcaagg cggcgctcca aggagaacgc
     1381 cgaggctacg aattctacta cgcggttgcg ggcaccactg cggaccccgc gatccgcaaa
     1441 gtggcgcgtg agttcgtgaa agaggaagcc cagcacgtag acactttgaa gctctggatc
     1501 gcaaaggagg aagccgcgtt gcgtgagaag gctcgcgcgg cgggctgaac cacgggcggc
     1561 gctggcgccg ggcagccccg tcgactcgac gtctcgagct aatcgttagc tccgaatcgg
     1621 agctgtaggg ctgcctggac catgcccgcg agcgcggctt gtctgaagat cgtgccgcca
     1681 gatccttcga tcgcgaagcg atagcgcgta atcgggagca ctcctcgcgc accgagcaag
     1741 agcgccgacc aacgggtgag gcggagatcc aagtcggcac cgagctccgc tcccagccac
     1801 gcgcgctctc cgggttgact gcgctcgccg ctgaggagtg cggcgtgcat caaacctagc
     1861 agggggcctg cacaaacatg gataagcatg gcggtgctcg cgggaaggcc ggcgcaaagc
     1921 tgcaaagcgc ccgccgccgc gccgatttgg aagttgaact ccccattcga tccgcgcacc
     1981 gccgggaaca cccgcgcctg aactccgaca tcgaaagtcg aactgagcgc cgccgccgcc
     2041 agtagccccc caccaaagga tacgtgcggc agaagtcccg cttgcgcaac cgcgcccgcg
     2101 gccacgcgac ccgatggcga agtacgaggc ggcgcttcgc gtgggacgtc tggggcccgt
     2161 gcgggcacct cccgagacac cggcgtcggc gcaggagctg cattcgccgg ggcttgcgtc
     2221 gcgggagggg aggcctcact cgccgaagcc gcgagcgcgg cctctgggtc aatcgcgaga
     2281 gctacggcaa gcacgaccgc gtcgcccaag gactcgcagc cttgtgaagt gctcttgagc
     2341 tcgcgtacgg ccggctcggt atccgtcgcg tgagctcgga ccgcaatctg ggcgcgccag
     2401 gtgccggctt ctcttcgcac catgccttcg atgctgcgct gggcttgcgg ttggaacggg
     2461 tcgttgccca ggcgcctccg cacgcgctcc gaaaggctgg aggcgtcgat acagctaccc
     2521 gcaccctcga gtcgcaccca ctgcagacgg accggttcgg aggcgccggc ctcgcgcgcg
     2581 gcggtcaatg tcaggcaaag cagtgacgct ctgaggatcg cgcgggatgg agctatgggg
     2641 actgcagcgc ggtcgcacat aaggcggatg cgttgcagta gggggcggtg catgttccag
     2701 agttcgcgaa gcggaagtgg acctggcggg tgcgcgtcgt gcaaacgtcg agaccgggct
     2761 cttccggtcc gcctatgttc aagctcaagt tccaaccgat ctcggcgccc gacgcaaggg
     2821 cccagctggg caggtcgaga tcgttgctca cgatgaacgc ttcgaccgca taaccactcg
     2881 gagtcggggc ggccgtaaag cgcgtggacg cccaggcccc gaggtcgaca ccgtctccgg
     2941 acccagggga cacgaaacgc tgcccacgtc ggaccgcggc ggtttcactg cttggcgccg
     3001 ctacgacgaa ctgcatggtg ccgggccgat cataggcggg gggcgagcgg atcgtgccat
     3061 cgtcatcgac atacagctcc accccgtcac cacaataggc gagtgcgtcg agcggcgccg
     3121 ggtctcgcgt cgggtcggtg accgcgacga agaagtagag cccatccgag cggtacgcga
     3181 ggctcaagtc ggccctcacc ccggcgttgc tgtccgacgt cgtttcatcg atccatgagt
     3241 acagcgataa tcctgaatcc agtttgccgt cgatgatcgg ctcgctcgcg agcgggggta
     3301 tgcgatcaca gagtagtgcc gcactcatga gcccaggatc cacgctgccg cctgcccccg
     3361 cagaattctc tcgcgctaag ctccaatcca tcgagcaagc caatacactg aagctcgata
     3421 gcgcaagcgt cgatgttttc atggcgctcc gagcaccttg tccaagcgct cttcgtagat
     3481 gctattgggc gcggccttgc gtaggcctcg acccttggct tcggcctcgc gtcgacgccc
     3541 gagcttgacc agagcttgca cgagtatcaa ctcgcgttcg gccgcgagct gcccgcgtgg
     3601 atagcgcttg gcgtgctcgc gagcgatcgc gagtgcgcgg ttcggatccg tagacagcgc
     3661 gcggtgcgcc tcgttgagaa gctgcgtttc tccagacagc gtgtcttctt ctgacgacgg
     3721 ttgcggggcc gagatgaccg aagcccctct gccatggggc cgtgttggag gctccgcgct
     3781 cgcgatcgga gagccttcca ggctcaccgg ggc
//
LOCUS       DASTKU010000138         3525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_247429, whole genome shotgun sequence.
ACCESSION   DASTKU010000138 DASTKU010000000
VERSION     DASTKU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247429"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VFQ61_04870"
     CDS             complement(<1..920)
                     /locus_tag="VFQ61_04870"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HET9953807.1"
                     /translation="MSSPRWRGLWGARRLLPGSRAGASALGVLAAAALFVNVNLLVSR
                     FTQRWDVTRERLYSISEPTRATLQGLSQEVELIVLLGRADPLWRSIRQLLSAYTSESP
                     KLQVRYLDPEQNPAEFLALAQRYGIVSGKAEDGRVVTDASIVIAQGERHWFITQEQLL
                     GFDIESGNLRPALEQALTQGIANVRGQERAKVCFSRGHGELSLFDAGPDGMLELRERL
                     TKNNYEVEERDLPGSQKQPGLEGCRLAIVAGPTRPFEPAAADAVTAAMRAGTSALLLL
                     SAVAADDGSIASSGLDPVLRLGELELGRDLV"
     gene            complement(917..1666)
                     /locus_tag="VFQ61_04875"
     CDS             complement(917..1666)
                     /locus_tag="VFQ61_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9953808.1"
                     /translation="MTAFWAIYRREMLGLWVTPLAWVLWVVFLLLEGGIFYSIVIHFT
                     TLPEISVDSGPLQAFFGQESILLLLTLILLCPALSMRLFAEERARGTIEPLLTAPVSA
                     TSVVLGKYLAVLSTYVGMWLPTLLYVLILRRAGSVDPRVVASSYAGLFGVGAAYLAIG
                     TLMSTLTKSQLVALLLTLLFVFGMFVMGIGEYVFAEGLFHDLCAHVSVAAQMEEFSRG
                     IVESRRVVFDASIVALCLFLSVRVVDSWRWG"
     gene            complement(1663..2640)
                     /locus_tag="VFQ61_04880"
     CDS             complement(1663..2640)
                     /locus_tag="VFQ61_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9953809.1"
                     /translation="MIEVQHLTKLYGAVRAVDDLSFSVGAGEVVGFLGPNGAGKSTTL
                     RILAGFLGATSGLVRIDGHDVERAPIEAKRKIGYMPESAPLYPELRVQEYLAFRVALK
                     GVPRRERKAAVQSAMANAHVVDVSETLIGHLSKGYRQRVALADALLGSPPLLILDEPT
                     VGLDPNQIREVRHLIRELGQTHTILLSTHILSEVEATCDRAIVVSRGHLVAEGRLAEL
                     AKLGKSQSLRAELRDPRGCARELAANIEAVLSVEDVSASSASEVPSKDAVITLHIVVR
                     EGPESVAERLCAEWVRAGVFVRRIEPVQASLEQVFAELTASEKSAPEAA"
     gene            complement(2718..3182)
                     /locus_tag="VFQ61_04885"
     CDS             complement(2718..3182)
                     /locus_tag="VFQ61_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9953810.1"
                     /translation="MVLVSAGVLLYRRVASNLQVLLAHPGGPHFVRRDRGAWTIPKGA
                     PLEAEALEDAARREFQEEVGLPLGTSWLALGSVRQKSGKIVHAWAVEGTAPDGYTPAS
                     NEFEMEWPPRSGRFQKFPEVDRVEFFELHVAEEKIIEAQRPFLTRLSSVLGV"
BASE COUNT          711 a         1199 c         1048 g          567 t
ORIGIN      
        1 accaagtcgc gtcccaactc gagttcgccc aagcgcagca cggggtcgag cccgctgctc
       61 gcaatgctcc cgtcatcggc cgccaccgcg gacaacagca gcagcgcgct agtcccggct
      121 cgcatcgcgg ccgtcaccgc gtcggcagcg gcgggttcga acggcctggt cggcccggcc
      181 acgatcgcca accggcagcc ctcgagacct ggttgctttt gggagccagg tagatcccgt
      241 tcctcgacct cgtagttgtt cttcgtcagg cgctcccgca actcgagcat accgtccgga
      301 cccgcgtcga acagcgagag ttcgccgtga cctcggctga aacaaacctt ggcgcgctcc
      361 tgcccgcgaa cattggcgat gccttgggtg agcgcttgtt cgagcgccgg acgcaagttg
      421 ccggactcga tatcgaagcc gagcagctgc tcctgcgtaa tgaaccagtg tcgctcaccc
      481 tgggcaatga cgatgctggc gtcggttacg acccgaccat cctcggcctt gccggagacg
      541 ataccgtaac gctgcgccaa tgccagaaat tctgcgggat tctgctctgg atcgaggtaa
      601 cggacctgca acttcgggct ctcggacgta tatgccgaga gcagctgtcg aatactgcgc
      661 cacaacggat cggcacgccc caagagcacg atcaactcca cctcttggga gagcccctgc
      721 agcgtggcgc gcgtgggttc cgagattgaa tacagccgtt cccgggtcac gtcccaacgc
      781 tgagtgaacc gtgagaccag caaattgacg ttcacgaaca aagccgccgc cgccagaact
      841 cccagcgcgc tcgcgccggc gcgtgacccg ggcaacagac gccgagcgcc ccaaagtccc
      901 ctccaacgcg gagagctcat ccccacctcc aggagtccac gacccgcacg ctcaagaaca
      961 agcaaagtgc cacgatcgag gcgtcgaaca ccacgcgccg cgactccacg atgcccctgg
     1021 agaactcctc catctgcgcc gcgacggaga cgtgcgcgca caaatcatgg aacagccctt
     1081 cggcgaagac gtattcaccg atgcccatca cgaacattcc aaacacgaac aacagggtca
     1141 agagcagcgc cacgagctgg ctcttcgtca aagtgctcat caacgttcca atggccaggt
     1201 aggccgcgcc gacgccgaac agccccgcat agctcgaggc caccacacgc ggatcgacgc
     1261 tgcccgcacg acgtaaaatc aaaacgtaga gtagcgtcgg caaccacatg ccaacgtacg
     1321 tgctcagcac cgccagatat ttgccgagca cgaccgaggt ggccgacacc ggcgccgtga
     1381 gcagcggctc gatggtgccg cgggctcgct cctcggcaaa cagccgcatg ctgagcgccg
     1441 ggcacagcaa gatcaacgtc aagagcagga ggatcgactc ttggccgaag aatgcctgaa
     1501 gcgggccgct atccaccgag atctccggca gcgtcgtgaa gtgaatgaca atcgaataga
     1561 agattccgcc ttcgagcagc aggaacacga cccacagcac ccacgcgagc ggggtaaccc
     1621 acagccccaa catttctcgc cgatagattg cccagaaagc ggtcatgccg cctccggcgc
     1681 cgatttctcg gacgcggtca gctccgcgaa gacttgctcg agcgatgcct gcaccggctc
     1741 gatgcgacgc acgaagaccc ccgctcgaac ccattccgca cacaggcgtt cggcgacgga
     1801 ctctggccct tctctcacta cgatgtgcag cgtgatcacc gcgtctttgc tgggcacttc
     1861 cgaagccgac gaggcggaga catcctcgac ggacagcacc gcttcgatgt tcgcggcgag
     1921 ctcgcgggcg cagccccgag gatcgcgtag ctcggcgcgg aggctctggc tcttgcccag
     1981 cttcgcgagc tccgcgaggc gcccttcggc gaccaggtga ccccggctca ccacgatcgc
     2041 gcggtcacaa gttgcctcga cttcggacaa gatatgggtg gagagcaaga tggtatgggt
     2101 ctgcccgagc tctcggatca gatgtcgcac ctcacggatt tgattcgggt ccaaacccac
     2161 cgtgggctcg tccaagatca gcaaaggcgg cgacccgaga agcgcgtcgg ccagcgccac
     2221 gcgctgtcga tagcccttgg agaggtgccc aatcagagtc tccgagacgt ccaccacgtg
     2281 agcgttcgcc atcgcactct gcaccgcggc cttgcgttca cggcgcggca cccccttcaa
     2341 agccacccga aacgccaaat attcctgaac tcgaagctcg gggtagagcg gagcggactc
     2401 gggcatatag ccgatcttcc gtttggcctc gatcggcgcg cgctcgacgt catggccgtc
     2461 gatgcgtacg aggccactcg tggcaccgag aaaacctgcg aggatacgta gggtggtgct
     2521 cttgccggcg ccgttcgggc cgagaaaacc cacgacctcg cccgcgccca cgctgaacga
     2581 gaggtcgtcc accgcgcgaa ccgcaccgta tagcttggtg aggtgctgga cctcgatcat
     2641 ggagcgcgtc ctaccacgat tccgggctca cgattccggc ccggtccggc gttacggcga
     2701 acccggtaag aatcgcgcta aacgccgagt accgagctca gccgggtcaa gaacggccgt
     2761 tgtgcttcga tgatcttctc ttccgccacg tgcagctcga agaactcgac ccgatcgacc
     2821 tcgggaaatt tttgaaagcg cccggagcgc ggcggccact ccatctcgaa ctcgttgctg
     2881 gccggtgtat agccatctgg agcggtgcct tctaccgccc aagcgtgcac aatcttgcca
     2941 ctcttctggc gcacggaacc caacgccaac catgacgttc cgagcggcaa gcccacctct
     3001 tcttggaatt cgcgccgagc cgcgtcctcg agcgcttcgg cttcgagggg ggctcccttc
     3061 ggaatcgtcc acgcgccccg atctcggcgc acaaagtgcg gcccgccggg atgagcgagc
     3121 aacacttgga ggttggaggc aacccggcgg tataggagca cgccagcgct gacgagaacc
     3181 atgcgggaag tctttcgtct gaccgggaga gtatgtcagg cgcccagaac aagagaagta
     3241 caatgctgaa attccggccg cggtccgcct gccccaagct tgggtccgct cggatcgagc
     3301 aaagaatcgg cggcgcggtc ggccttacgg ctcgtggcta gccgcgtagc ggggcctgcc
     3361 cgggggattc gttgcgagcc gccaatgaag cgtgatgcgg ctcgcttgac gcttgacgag
     3421 cggccactcc gtcgtctggg caccgtctcg agaaactaga agtcgcgctg gccgtcaccc
     3481 gtatcgatga actcgaccgc cacgactgcg gtcgcggtca gtcgc
//
LOCUS       DASTKU010000139        13204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_695063, whole genome shotgun sequence.
ACCESSION   DASTKU010000139 DASTKU010000000
VERSION     DASTKU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13204
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695063"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFQ61_04890"
     CDS             complement(<1..328)
                     /locus_tag="VFQ61_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9953811.1"
                     /translation="MDAAREVQAGNLAARAPALDSLNELRAQYRRNPTPFSAEVLGML
                     KQVSQNLAAVPPTSVLERMLKETFGYDHFRPGQLPIIESLLKGVDCVGVMPTGAGKSL
                     TFQLPAR"
     gene            527..1444
                     /locus_tag="VFQ61_04895"
     CDS             527..1444
                     /locus_tag="VFQ61_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HET9953812.1"
                     /translation="MAVPELSDHTPTVVPDTSRQSPARRLRNLLARDGLILMPCGFDA
                     LSLRLIEMAGFDLGFMSGFGVSAARIGAPDTGLISYGEMLDQGRNICAAVQIPVIGDG
                     DTGYGNAMNVQRTVRGYAQVGFAAVMIEDQLAPKRCGHTRGKQVVGRAEALNRIRAAV
                     DAREAGADVLVLARTDARATLGLDEALYRARAFADLGADIVFVEAPENPEEMREISRQ
                     VRRPLLANMLEGGRTPWLTPAALTDLGYKIVAYPLTLLSVATRAMQAALAELRRGDPA
                     SGILPFEELQRVVGFDAYDAAAVRYKEGH"
     gene            complement(1483..2241)
                     /locus_tag="VFQ61_04900"
     CDS             complement(1483..2241)
                     /locus_tag="VFQ61_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9953813.1"
                     /translation="MSVSEKPPLPEKTHALDEPGRPETRPEKTQKIVEARMALRERFL
                     ARIEATPSLADSEPQGTGPRNRHGMPALPVGQVATHKWPVLDLGVHPEVPLTEWSLTV
                     DGVVRKPLRLSFADFMALEQTEDVSDFHCVTTWSKLDMHWRGVRVSDLLCLAEPLEQA
                     QYLMCHAYDGYTTNLSLSEALKDDVLLVHTVDGGPLSREHGGPVRMITPQLYAWKGAK
                     WINRLEVLPQDQPGFWEQRGYSMTAYPWRNDRYG"
     gene            complement(2264..2926)
                     /locus_tag="VFQ61_04905"
     CDS             complement(2264..2926)
                     /locus_tag="VFQ61_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9953814.1"
                     /translation="MTISVVTGANRGIGLEFCRQLKARGHSVLAACRKSSPELEALGV
                     EIQSGVDVAREEGVKRLAQAVGTRELTLLVNNAGILDQDSIGRLDYGSIRAQFEVNAL
                     GPLRVTEALLPRLVSGAKVALITSRMGSIGDNSSGNYYGYRMSKAALNMAGVSLAHDL
                     RSRGIAVAILHPGFVRTEMTGSSGGLEPEESVRGLLARLDALTLSTSGTFWHMNGEHL
                     PW"
     gene            2996..3817
                     /gene="tsaA"
                     /locus_tag="VFQ61_04910"
     CDS             2996..3817
                     /gene="tsaA"
                     /locus_tag="VFQ61_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HET9953815.1"
                     /translation="MTDEPSFEASTEVLCVRPIGVVHSPFQERVGAPRQPAAARGVAG
                     TVELHASSGIEHALLDLESFRFIWILFWFHKNHGFRPKVLPPRSTERRGVLATRSPYR
                     PNPIGLSAVELVRIEGLVLHVRNLDLLDGTPVLDIKPYVPYTDSIPDANSGWLENAAT
                     PSDPLPTYRVEFSAECLQQLDFLDAQFGIVLRDKVESVLSLGPQPHPYRRIKRDGERW
                     VLAHKEWRFNFDVSGTSIRVTRVFSGYRPSELFGSPKPGLEAHVALMTAFSAGPR"
     gene            3919..4347
                     /locus_tag="VFQ61_04915"
     CDS             3919..4347
                     /locus_tag="VFQ61_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HET9953816.1"
                     /translation="MAIAKAKAKWSGTLKEGNGSMSSGGGFFELPFTFASRFEGAANA
                     TNPEELIGAAQAGCFSMFLAAQLTNAGKPPKRIETEATVHLGAGPAITKIELTLEAEV
                     PGLDQALFSEKVEFSKKNCPVSKALTGVPELVVNAKLVAG"
     gene            complement(4364..5278)
                     /locus_tag="VFQ61_04920"
     CDS             complement(4364..5278)
                     /locus_tag="VFQ61_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HET9953817.1"
                     /translation="MSEHSHGWQARLDLELIHEAPRTRLGHRSHFGPLRIQRPFYPEG
                     EDRLHLYLLHPPGGLVGGDSLEISVTSRAGAHGLLTTPSAQKVYRTIESRSSQRVVLH
                     VEGHASLEWLPSETLIFDGAAARLETRVELAKEASFIGWEIGCFGRPASQAPFAQGDL
                     FQSFEIYCAGEPVIVDRLRMRGGSEALQAAYGQQGYPAYGTLYCVVPSAVFTSSAAAS
                     NSSSVLGDGSAPNQTPGPASSTADWLTTLRGRLGEQARATAFENAVVVRARAEHVELV
                     RHELVRAWACLRELALGRPPSLPRIWAT"
     gene            complement(5346..5942)
                     /locus_tag="VFQ61_04925"
     CDS             complement(5346..5942)
                     /locus_tag="VFQ61_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953818.1"
                     /translation="MPAGSPTIPTLAEVEPEDDASPTGAVGCAESIDRLRVGTGLAAE
                     PSARAEEGDEEGDDRRVADAAGFEIGGSAGRGDSGSAAGSGVARTTGEGGSGCGGSGC
                     GGSRRGGAFGAGSGEAGTLGRGSRGGSTGAPDADAVRGASLSTSTGTASQTSGSQACG
                     ESLENRCDPMFPSSAAAWSNSDNNKGTPKGNGLAIGTR"
     gene            5941..6384
                     /locus_tag="VFQ61_04930"
     CDS             5941..6384
                     /locus_tag="VFQ61_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953819.1"
                     /translation="MRHFAKAFARALPAGCYADAGWLELPLGPVGRAVFAVHIDDTGH
                     IEQVERDPDVPAPAIIQRMIERAIVLLRAGTFSLEPGRVSAGIEWMELHVVLSQTAAN
                     TDPDANGRHLRAEGHETPTWQRPGRGYFTLNSGLHMEASLRLLRR"
     gene            6601..7704
                     /locus_tag="VFQ61_04935"
     CDS             6601..7704
                     /locus_tag="VFQ61_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9953820.1"
                     /translation="MAHGPLALVLLGAGLTGAACSADATPIVRRTADSLSGGDAGSGD
                     TSIGGACATQSAEAMLLPLYLAFAFDVSGSMGKGDQPWHDRTLKWDPVVSATRAFFED
                     AQSSGIHASLTFFPSAEGDDSVRCDSKVYESPDVPMSALPSTAFGKALDDIGQQEWRG
                     STPTQYVMRGVLNVVRAAMVRQPGKYALVLVTDGYPQDCDDNSIEAVAAVAAEARREI
                     PTYVLGVKNPPIEGAPDVTSNLNQIAIAGGTEQVFMIGTGDPASTITTFRAAVDSIRG
                     ASLVCDVGIPAPPVGSVFDKERVVVRYSSENTETHLVYDASCLKEGAWHYDDANRPAR
                     IVLCPSTCAAVQPPNAASINVEFSCEKKIDIPI"
     gene            complement(7868..10711)
                     /locus_tag="VFQ61_04940"
     CDS             complement(7868..10711)
                     /locus_tag="VFQ61_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9953821.1"
                     /translation="MTAARYIVGIDLGTTHTVVAFAEFEGQSPPEVFRVPQLVTAGEV
                     EPRVLLPSFLYTPLPEEIPATELDEGWIVGEFARKRGVEVLGRGIASAKSWLCHSAVD
                     RLAPILPWGADANASSNEDVSAEPTEAFAIRRLSPVAASTLILKHVARAWDASHPAAP
                     LRQQTVILTVPASFDGVARELTVAAAQAAGLTVRLLEEPLAAFHDYLSRFGLAPLSNL
                     LQAEPELDILVCDVGGGTTDLTLVRAQTGSGSDPSFTRSAVGRHLLLGGDNIDLALAH
                     RVERRLLPPGEHLDPARFTALLMACRQAKEQLLSRASEAVESSNTEFPIRLLASGSSL
                     VGSTLASSISRAELSELVLDGFLPFVRPGQEARVRRAGLSGFGLPYESDPAITRHVVA
                     FLERHLGGTRGPGALLLNGGLFRAPTTRARLAEVLRQRYGRDIELLPGIDPDLAVARG
                     AVAYGRELLGDARARSRVRAGASHGYYIGVGGAELNRLAICVVPRGALPEENHVCQTR
                     PLVLRLGEPVRFELYASDTRPHEAPGTLVTIDDEQFVRLSPLVTRLQSPSENQREMRV
                     FLEGKLSAIGTVELSCVEAATPSSTPAPTSTSALTSAQPAKQHYRLAFEPSSRPSESQ
                     RPPGRRAEVPHPRWDEAILLLDRVFGKSRSDVKPREIKDLLRELERVLGPRSQFSFEV
                     GRAIADFLLERASARKRSPDHERLFWMLTGHGLRPGFGHPRDPDRLRGLTPLLGTGLV
                     FQAEARGYQQFWIAWRRIAGGLDEPTQTELRKIADPWLAPAELKLKKPKGFKPQALDE
                     LADLSSWLERVPAESRSELGRYFLERTWTDRDPRIWTWIGRIGARVPAYASAHYVVRP
                     SVIERWLDHLLREDWDRVPTAAETAAKLSRVSGDRARDLSEPVRQEVIRRLQRCAAPE
                     PLIQMVREYVKVESSERAAWFEDLPVGLMLAEA"
     gene            10788..11483
                     /locus_tag="VFQ61_04945"
     CDS             10788..11483
                     /locus_tag="VFQ61_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HET9953822.1"
                     /translation="MTYPFAGPKLRAVTESTHSSAGPLSFLERFLFAWQCLFRIWFDG
                     EFALRLKGAARGFEALPSPDASSPSDRDEPVESDDTAVESQEPSKEAESVRQEAEAQR
                     AREAARVGALQLLGLLQREGRFVDFVQQEIAKFSDAEIGAAARVVHAGCRKALLNHAR
                     VEPARPENEGARLTLDADFDRGRLKLTGNVGGKPPYQGTLRHRGWIVSDLALPILVEG
                     YDARVVAAAEVEL"
     gene            11480..>13204
                     /locus_tag="VFQ61_04950"
     CDS             11480..>13204
                     /locus_tag="VFQ61_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9953823.1"
                     /translation="MSEQRFTIGIDLGTTHTAMASASLETPDAKVSVFDVPQLVAAGS
                     VEARSLLPSFLYFAHESDAPLALPWATHDYAVGEFARARAAEAPGRVVSSAKSWLSHA
                     GVDRRSPLLPVTAAADIEKISPVEASFRYLDHLGEAFRSAHQGQLSLGEQEVVLTVPA
                     SFDAAARDLTVEAAYAAGLENVTLLEEPQAALYAWIEAHGEAFRTHLKPLDVVLVIDI
                     GGGTTDFSAIALLENQGSLELHRVAVGDHILLGGDNMDLLLAAVVRNKLRDAGTELDA
                     WQMTSLSQACRAAKEQLLAENPPESVPIVIPSRGSQLLGQSIRTELLRQEVERVILDG
                     FFPGVELAAKPTQRQRAGLAQLGLPYASDPAITKHLAHFLTRQAGALANLPGFPAASE
                     LLRPTSVLFNGGVVKSQAIRGRLLACLNQWLEASGAPPIRVLPGEDPDLAVARGAAYY
                     GKVRRGRGLRIRGGTARAYYVGIESPMPAIPGLEPPISALCVAPFGMEEGSQTELPKH
                     ELGVIVGEPVEFRFFGSSVRRGDSAGTLLEHGKSGELEELSPIGMTLVAEGRQKGEFV
                     PVHLRASVT"
BASE COUNT         2328 a         4341 c         4176 g         2359 t
ORIGIN      
        1 tgcgcgcggg cagctggaac gtgagagact tgccggctcc agttggcatt accccgacgc
       61 aatcgactcc tttgagcaac gattcgatga tgggcaactg ccccggacgg aaatggtcgt
      121 agccgaacgt ctctttcaac atccgttcca agacggaggt gggcgggacc gcagctaggt
      181 tctgtgagac ctgtttgagc atacccaaca cctcggccga gaacggggtg gggttgcgcc
      241 gatattgagc gcgcagctcg ttgagagaat ctagcgccgg ggcacgcgcc gcgaggttgc
      301 cagcctgaac ctcgcgcgcc gcatccacga ggcgcgttat acgttcggag agtgacgggt
      361 cggacagagc gcacggagcc tgactcgaaa ccctcgtcga agaaagggaa cagtgctcac
      421 ctcggcgggg ttcaccgtga agaattgggc ggcttgaggg ccggtctcgg ctggtggtgc
      481 ttaccccaaa atctgtttgc aaaggtgccc ttcgcagcga gcatgcatgg ccgtgcctga
      541 actcagtgac cacactccga ccgtcgttcc cgatacgagc cgccagtctc cagcccggcg
      601 gctgcgcaat ctactggcgc gcgatggctt gatcctcatg ccttgcgggt tcgacgcgct
      661 gtctttgcgt ttgatcgaaa tggccggctt cgatctgggg ttcatgagtg ggttcggggt
      721 gtccgccgct cgtatcggag cgccggacac cggtctgatt tcttatggcg agatgctcga
      781 tcaaggccga aacatctgtg ccgccgttca aatcccggtg attggcgacg gcgacaccgg
      841 atacggcaat gccatgaacg tgcaacgcac ggtacggggc tacgcccagg tgggcttcgc
      901 ggcggtcatg atcgaggacc aactcgcacc aaaacgatgc ggccacacgc gcggcaaaca
      961 ggtggtgggc cgagcggagg cgctgaatcg catccgcgcc gccgtagacg cgcgcgaggc
     1021 gggggcagat gtgttggtgc tggcgcggac tgatgctcgc gcgacgctgg ggctcgacga
     1081 ggctctctac cgcgcgcgcg ccttcgcgga tttgggagcg gacatcgtgt tcgtcgaagc
     1141 tcccgaaaat ccggaagaga tgcgggagat ctcgcgccag gtgcggcgac ccctgctcgc
     1201 gaacatgctc gagggtgggc gaacaccttg gcttacgccc gcggcgttga ccgacttggg
     1261 ttacaagatc gttgcgtacc cgctcacact gctcagcgtg gccacgcgcg ccatgcaagc
     1321 tgcactggcg gagctgcgtc gcggcgaccc cgcatccggg attctgccat tcgaggagct
     1381 gcagcgcgtc gtcggtttcg acgcctacga cgcggcagcc gtgcgttaca aagaaggcca
     1441 ctgacgttcg aacgaggcgc ggctcgtgcc gccgcaaacg cgtcaaccgt agcggtcatt
     1501 ccgccaagga tacgccgtca tcgagtaacc acgttgttcc cagaaaccgg gttggtcctg
     1561 gggcagcact tcgagtcggt tgatccactt cgctcccttc caagcgtaaa gctgcggcgt
     1621 gatcatgcgt acggggccac cgtgctcgcg tgacaagggg ccgccatcca cggtgtgcac
     1681 caacagcacg tcgtccttca gcgcttcgct caacgagaga ttggtcgtgt aaccatcgta
     1741 cgcgtggcac atcaagtatt gggcctgctc cagcggctcc gccaggcaca gcaggtcgct
     1801 cacgcgcact ccacgccagt gcatatcgag cttcgaccag gtggtgacgc agtggaaatc
     1861 actgacatcc tcggtctgct cgagggccat gaaatccgcg aacgagagcc ttagcggctt
     1921 ccgaacgacg ccgtccaccg tcaatgacca ctcggtgagc ggcacctccg gatgaacgcc
     1981 gagatccaac acgggccact tgtgcgtcgc gacttgtccg accggcagcg cgggcatccc
     2041 gtgtcgattc cgcggccccg tcccttgggg ctcagagtcc gccaggctgg gtgtagcctc
     2101 gatgcgagcc agaaagcgtt cacgcaaggc catgcgggcc tcgacaattt tttgcgtctt
     2161 ctcgggtctg gtttccggtc ttcccggctc gtccagcgcg tgtgtcttct cgggaagggg
     2221 aggtttctcg gacacggaca tcgggaacct ttcgattcgg cggctaccag ggcaggtgtt
     2281 cgccattcat gtgccagaac gtgccgctcg tcgagagcgt gagcgcgtcg agccgcgcca
     2341 acagccctcg cactgattcc tccggctcca ggccgccgct ggatcccgtc atttcggtgc
     2401 gcacgaaacc cgggtgcaaa attgcgactg cgatcccgcg gctgcggaga tcgtgcgcca
     2461 ggctcacgcc agccatgttg agcgccgctt tggacatgcg atatccgtaa tagttgccgg
     2521 agctgttgtc cccaatcgat cccattcggc tcgtgattaa agccactttc gcgccggaca
     2581 ccaagcgcgg caacagagcc tcggtcaccc gcagcgggcc cagcgcgttc acctcgaatt
     2641 gcgcccggat cgagccgtaa tccagacgcc caatcgagtc ctgatccagg atgccggcgt
     2701 tgttcacgag cagcgtgagc tctcgggtgc ccaccgcttg agccaggcgc ttcactcctt
     2761 cctcacgggc tacgtccact ccactctgga tttcgacccc caaggcttcg agttcaggcg
     2821 agctcttgcg gcaagcggcc agcaccgaat gcccgcgtgc cttgagctgt cggcaaaact
     2881 cgagtccaat tccgcggttc gctcccgtga cgacggagat ggtcataagc ggcacgatga
     2941 cagcgcttta tgtgcgcgac aagcgcgttt tccgagtgta cgaagtccac ccgccatgac
     3001 tgacgagccc tcgttcgaag cttcgacgga agtgttatgc gtgcgcccga tcggcgtggt
     3061 gcattcccca tttcaggagc gtgtgggtgc cccgcgtcag ccggcggccg cgcgtggggt
     3121 agcggggacc gtcgaattgc atgcgagttc gggcatcgaa cacgcgctct tggatctcga
     3181 gagttttcgt ttcatctgga tcctgttttg gtttcacaaa aatcatggtt tccggcccaa
     3241 ggtcttaccg ccgcgcagca ccgaacggcg cggggtgctg gcgacccgct caccctatcg
     3301 accgaacccg atcgggctca gcgcggtgga gctcgtgcgc atcgaggggc tcgtgctgca
     3361 cgtgcgaaat ctcgatctcc tggacggcac cccggtgctc gatatcaaac cgtacgtacc
     3421 gtatacggac tcgatcccgg acgcgaacag cggttggttg gagaacgcag cgacgcctag
     3481 tgatccgctc ccgacctatc gagtggagtt cagcgccgag tgcctgcaac agttggattt
     3541 tctggatgcc caattcggca tcgtgctgcg cgacaaggtc gagtccgtgt tgtcactcgg
     3601 ccctcaaccg catccctatc ggcgcatcaa gcgtgacggc gagcgttggg tgctggctca
     3661 caaggaatgg cgcttcaatt tcgatgtttc aggaacatcc atccgcgtga cccgcgtctt
     3721 cagcggttat cggccgagcg agctgttcgg ctcgccaaaa ccagggctcg aggcgcatgt
     3781 ggcattaatg accgcctttt cggccggtcc acgttaaccg gaagatccgg cgatcgcgac
     3841 gatagtcggt gcggcaactc gcgaacccga ctaagaagct cgcaaacgga ctctcgcgtc
     3901 cgagaaagtg aaagacgaat ggcaattgcg aaggccaagg ccaagtggag tggaaccctc
     3961 aaggaaggca acggctcgat gagcagtggc ggcggtttct tcgaactgcc gttcacgttc
     4021 gcttctcgct tcgaaggcgc tgctaacgcc accaaccccg aagagctcat tggcgcggcg
     4081 caagcgggct gcttctcgat gttcttggcg gctcagctca ccaacgcggg caagcccccc
     4141 aagcgcatcg agaccgaggc cacggttcac ttgggtgcgg gtcccgcgat cactaagatc
     4201 gagctcacgc tcgaagccga ggtcccgggg ctcgatcagg ccctattctc cgagaaggtc
     4261 gagttctcga agaagaattg ccccgtttcc aaggccttga ccggcgttcc agagctcgtc
     4321 gtcaacgcca agctcgtcgc cggctgaggc tcgcgtccag aagttaggtc gcccaaatcc
     4381 gaggcaggga cggcgggcgg cctaacgcca actcacgcaa gcaagcccaa gctcggacga
     4441 gctcgtgcct aacgagctcg acgtgctccg ctcgggctcg aacgacgacc gcattctcga
     4501 aggcggttgc gcgcgcctgt tcgcccagac gcccccgcag cgtagttagc caatctgccg
     4561 tcgaactcgc cgggccggga gtctggttcg gagcggagcc gtcgcccaga acggagctag
     4621 agttcgaagc cgcagcgctc gatgtaaaca cagcgctcgg gacgacacag taaagggtgc
     4681 cgtaagccgg atagccctgt tgcccgtacg ccgcctgtag cgcctccgaa cctccgcgca
     4741 tccgcaggcg atccacgatc acgggctccc cggcgcaata gatctcgaag ctctgaaaca
     4801 gatcgccctg cgcgaacggt gcctggctcg cagggcggcc aaaacagccg atttcccagc
     4861 cgatgaagct cgcttctttc gccagctcga cgcgcgtttc gagtcgtgcg gcggcgccgt
     4921 cgaagatgag ggtctcgctc ggcaaccatt cgaggctcgc gtggccttcg acgtgcagca
     4981 caacgcgctg actactgcgg gactcgatgg ttcgatatac cttttgcgcc gaaggcgtgg
     5041 tgagcaagcc atgcgcgccg gcgcggctcg tcaccgagat ctcgagcgag tcccccccta
     5101 cgaggccgcc gggcgggtgg agaagataca ggtgcagtcg gtcctcaccc tccggataga
     5161 aggggcgctg gatccggagt ggcccgaagt ggctgcggtg tccgagccgc gtgcgtgggg
     5221 cctcgtggat tagctcgaga tcgagccggg cttgccagcc gtgcgagtgc tcgctcattc
     5281 gtgggccgac cttagctcga accgcctcgg aacccaacgt cggctatgct tggcgtgtcc
     5341 gaacctcacc gcgtgcctat cgccaagcca tttcctttgg gtgtgccctt gttgttgtcg
     5401 ctgttgctcc acgccgcggc gctgctcggg aacattgggt cgcatcggtt ttccaaggac
     5461 tctccgcaag cttggcttcc ggacgtctgg gacgccgtgc ccgtcgaggt gctgagcgaa
     5521 gcccctcgaa cagcgtcggc atccggagca cctgtagagc cgccgcgcga accacggccg
     5581 agcgtgcctg cctccccaga gccggcgcca aacgcgccac cgcgcctcga gccgccgcat
     5641 cccgagccgc cgcatcccga gccgccctcg cccgtcgtcc gagcaacgcc cgagccagca
     5701 gcgctgcctg aatcaccgcg ccccgcagag ccgccaattt cgaagcctgc cgcgtcagct
     5761 actcgccggt catctccttc ctcgtcgcct tcttccgcgc gagccgaggg ttcggcggcc
     5821 aagcctgttc cgacccgaag cctatcgatc gactcagcac aacccaccgc gccagtcgga
     5881 gaagcatcgt cttcgggttc gacctcagcc aacgtgggaa tggtcgggct acccgcgggc
     5941 atgcgacatt ttgcaaaggc ctttgctcgc gccctgcccg cgggttgcta tgcggacgcc
     6001 ggctggctcg agttgccgct gggccccgtt gggcgtgccg ttttcgccgt acatatcgac
     6061 gacacggggc acatcgagca agtggaacgg gatccggacg tgcccgcccc cgccatcatc
     6121 cagcgcatga tcgagcgtgc gatcgtcttg ctccgagccg gaacgttctc gctcgaaccg
     6181 gggcgcgtca gcgccggcat cgaatggatg gaactccacg tggtgctcag ccaaaccgcg
     6241 gccaacaccg acccggacgc gaatggtcgg caccttcgag ccgagggcca cgagactccc
     6301 acctggcagc ggccggggcg cggctacttc acgttgaact ccgggctgca tatggaggcc
     6361 tctttgcgcc tgcttcgacg ctgatgcgaa gttctgcggg gctcattttg aacctcgaaa
     6421 gcaaaattcg ccgagtcgac cgcggccacc ttgtggcagg agtctttctc ctggaaagcg
     6481 gcgcctttgt gcgagcggcg ggcattgcgt gagccatact ctgcgcgtcg aatgctcgct
     6541 cggttcggaa atcgcgcctt cgctcattgc caatccatcg gtcgcagatg cctagccgtc
     6601 gtggctcatg gcccgttggc gctggtgctc ctgggcgcgg gcttaaccgg cgcggcgtgc
     6661 agcgccgacg cgactcccat cgtgcgccgt accgcggatt ctctctcggg gggtgacgcg
     6721 ggctcgggcg acacatccat cggtggcgcc tgcgccacgc aatcggcgga ggccatgttg
     6781 ttgcctctct acctggcgtt tgcattcgac gtctcgggca gcatggggaa gggtgatcag
     6841 ccctggcacg accgcacact gaagtgggat cccgtggttt ccgccactcg agccttcttc
     6901 gaagacgccc aatccagcgg aattcatgct tcgctcacct tcttcccgtc cgccgagggc
     6961 gacgacagcg tgcgttgcga cagcaaagta tacgagtccc ccgacgtacc gatgagcgcg
     7021 ctgccgtcta ccgccttcgg caaggcgctc gacgacattg gacagcaaga gtggcggggt
     7081 agcactccca cccaatacgt catgcgcggc gtgctcaacg tcgtgcgagc cgccatggtt
     7141 cggcaacctg gcaagtacgc actggtgttg gtgacggatg gctatccgca agactgtgac
     7201 gacaacagta tcgaagccgt tgccgcggtg gcggccgagg ctcgccgcga aattcccacg
     7261 tacgtactcg gggtcaagaa cccgcccatc gaaggggccc ccgacgttac ctccaatctg
     7321 aatcaaatcg ccatcgccgg cggcaccgag caggtgttca tgattggaac cggtgatccc
     7381 gcgagcacca tcaccacgtt tcgggccgcc gttgattcga ttcgcggcgc gagcctggtt
     7441 tgtgacgtcg gcattccggc gcccccggtg ggcagcgtgt tcgacaaaga gcgcgtcgtc
     7501 gtgcgctaca gctccgaaaa cacggagacc cacctcgtgt acgacgccag ttgtctgaaa
     7561 gagggcgcct ggcactacga cgacgccaac cgccccgcgc gcatcgtcct gtgcccgagc
     7621 acctgcgcgg cggttcaacc gcccaacgcg gcttccatca acgtcgagtt ttcgtgcgag
     7681 aagaagatcg atatcccaat ctgaatctgc tccgctccaa ctcgagctcg agaattttcg
     7741 attcgtgctc cgcagccgcc gaacaacggg cgcgccgcga gtcagaagac gtttcgagcg
     7801 cagcccatgg tgcgcccgtt ccgtccccgc accgcgctcg ggccaccctg tagactgcac
     7861 acgaaaccta ggcttcggcg agcatcaacc ccacgggcag atcctcgaac caggctgccc
     7921 gttccgaaga ctccacctta acgtattcgc ggaccatctg aatgagcggc tctggcgcgg
     7981 cgcaacgctg caaacgccga atcacctctt gccgcaccgg ctcggataag tcacgcgctc
     8041 gatcgccgct cacccgtgac agcttcgcgg ccgtctcggc tgccgtcggc acgcgatccc
     8101 aatcttccct caagagatgg tccaaccaac gttcgatcac actcgggcga actacgtagt
     8161 gggcgctggc ataagccggc acgcgcgcgc cgatccgccc aatccaggtc caaatacgcg
     8221 ggtcacgatc ggtccaggtc cgttcgagaa agtagcgtcc caactcgctg cgcgattcgg
     8281 cgggcacgcg ctcgagccaa ctcgatagat cggcgagctc gtctagtgct tgcggcttga
     8341 accctttcgg cttctttagc ttgagctcag ccggcgcgag ccagggatcc gcaatcttgc
     8401 ggagctcggt ctgggtgggc tcgtccaggc caccggcgat gcggcgccaa gcgatccaaa
     8461 actgctggta gccgcgcgct tccgcctgaa ataccagccc ggtgccgaga agaggggtca
     8521 acccacgcag ccggtcggga tctcggggat gaccgaaccc aggacgcagc ccgtgaccag
     8581 tcaacatcca gaacagtcgc tcgtgatcgg gcgagcgttt gcgggcggac gcgcgctcga
     8641 gcaagaagtc agcgattgcg cgtccaacct cgaaagagaa ttgagagcgc gggcccagca
     8701 ctcgttcgag ctcccggagc aggtccttga tttcccgagg cttcacgtca ctgcggctct
     8761 tgccgaacac gcgatcgagc aacaggatgg cctcatccca gcgcgggtgc ggaacctcgg
     8821 cgcgccgccc ggggggccgt tggctctcgc tcggtctcga gcttggctcg aacgccagtc
     8881 ggtagtgttg cttggctggc tgcgcagacg tcagtgcgga cgtggaagtg ggagccggcg
     8941 tagagcttgg ggtcgcggct tccacgcaag acagctccac ggttccaatt gcgctcagct
     9001 ttccctcgag gaatacgcgc atctcgcgct ggttctccga cgggctttgc aagcgagtca
     9061 ccagcggtga gagtcgcacg aattgctcgt cgtcgatcgt gacgagcgtt ccgggcgctt
     9121 cgtgcggacg cgtgtcgctg gcatacaact cgaaccgaac gggctcgccg agccgcagca
     9181 ccagcggtcg cgtttggcag acgtggttct cctccggcaa cgccccgcgt ggcaccacgc
     9241 aaatggccaa gcggttcagc tcggcgccgc ctactccgat gtagtaaccg tgggacgcac
     9301 ccgcccgaac gcgactacgc gcccgcgcat cgcccagaag ttcacgaccg taggccaccg
     9361 cgccgcgcgc caccgcgaga tccggatcga tgccgggcag caactcgatg tcccgcccgt
     9421 agcgctggcg caacacctcg gcgagccgcg cccgggtggt gggcgcgcga aacaggccgc
     9481 cattgagcaa tagcgcgcct ggcccgcgtg ttccgccgag gtggcgctcc aggaaggcca
     9541 ccacgtgccg tgtgatggcg ggatccgatt catacggcaa cccaaagccg gatagacccg
     9601 cgcgtcgaac ccgcgcctct tgacctggtc gtacgaatgg caagaagccg tcgagcacga
     9661 gctcgctcaa ctcggcccgg cttatgctgc tagccaaagt cgagccgacc aggctcgatc
     9721 cgctcgccaa gagccgaatg ggaaactccg tgttggagct ctccacggct tcggacgcgc
     9781 ggctcaacag ctgctctttg gcttgccgac aggccatgag cagggccgta aaacgcgcgg
     9841 gatccaaatg ctccccgggc gggagcaagc gacgctccac gcggtgagcc aaggcaagat
     9901 cgatgttgtc cccgccgagc agaaggtgtc gtccgaccgc gctgcgggtg aagctcgggt
     9961 ccgaccccga tccggtttgg gcccggacca acgtcagatc ggtcgtcccg ccaccgacgt
    10021 cgcagaccaa gatatcgagt tctggctcgg cctgcaggag gttactcagg ggggcgagcc
    10081 cgaagcgcga aaggtaatcg tgaaaggccg cgagtggctc ctcgagcagg cgtacggtca
    10141 ggcccgcggc ctgcgccgcc gcgaccgtga gctcccgcgc gaccccatcg aaagaggccg
    10201 gtacggtcag aatcaccgtc tgttgtcgca ggggagcggc ggggtggctc gcatcccatg
    10261 cgcgcgccac atgtttcaaa atcaacgtgc tcgcagccac tggcgacagc cgccggatgg
    10321 caaacgcttc cgttggctcc gcggagacat cctcgttcga gcttgcgttc gcgtcggcac
    10381 cccagggcag aattggcgcg aggcggtcca cggcgctgtg acacaaccag ctcttcgcag
    10441 acgcaatccc gcggcccagc acctccaccc cgcgtttgcg ggcaaactcg ccgacaatcc
    10501 agccctcgtc cagctcggtc gcggggattt cttccggcaa cggcgtgtaa aggaaagagg
    10561 gcaaaagtac tcggggctcg acttccccgg ccgttaccag ctgcggcacg cgaaagacct
    10621 cgggcgggct ttgcccctcg aattccgcaa aagcgaccac ggtgtgcgtg gtgccgagat
    10681 cgataccgac gatgtagcgg gccgctgtca cactggcgtt ataccgcgcc cccttgcaat
    10741 gtgcgcgaac ccgcgggcgg gctcgcgaga ggatcggccg gcgccgcatg acttatccgt
    10801 tcgcgggccc taaactgcgc gccgtgactg aatcgaccca ctcctcagcc gggccgctgt
    10861 cctttctcga acgcttcctg ttcgcgtggc agtgcctgtt ccgtatttgg ttcgacggcg
    10921 agtttgcact gcggctcaag ggcgcagcgc ggggcttcga ggctctccca tcgcccgacg
    10981 cctcatcccc atccgataga gacgagccag tcgagtcgga cgacaccgct gtcgaatccc
    11041 aggaaccctc gaaagaggcc gagtcggtcc gccaagaagc agaggcgcag cgcgctcgcg
    11101 aagccgcccg cgtcggggcc cttcaactgc tggggttgct ccagcgcgag gggcgtttcg
    11161 tcgatttcgt ccaacaagaa atcgcgaagt tctcggacgc cgagatcggg gccgccgcgc
    11221 gcgtcgtgca cgcggggtgc cgcaaggctt tgctcaacca cgctcgtgtg gaaccggcgc
    11281 gcccagagaa cgagggcgcg cgcctgactc tggacgccga tttcgaccga ggccggctca
    11341 aactgaccgg caacgtaggg ggcaagccgc cctaccaggg aaccctgcgc catcgcggct
    11401 ggatcgtcag cgacttagcg cttccgattc tggtagaggg ctacgacgcg cgcgtggtag
    11461 cggccgcgga ggtagagctg tgagcgaaca aagatttacg attggcatcg atctcgggac
    11521 tactcacact gcgatggcat ctgcgtcgct cgagacgccg gacgccaaag tgagcgtctt
    11581 cgacgttccg cagctagttg cggctggtag cgtcgaagcg cgctctctct tgccctcgtt
    11641 cttgtacttc gcccacgaga gtgatgcgcc actcgcgcta ccttgggcga cccacgacta
    11701 cgccgtcggg gagttcgctc gtgcccgcgc cgccgaggcg cccggtcgcg tcgtgagcag
    11761 cgccaagagc tggctctctc acgccggtgt ggatcgccgc tcccccttgc tgcccgtcac
    11821 cgcggcggcc gacatcgaga agatctcgcc cgtagaggcc tcgtttcgtt acctcgacca
    11881 tctcggggaa gcgtttcgtt ccgcccacca agggcaactc tcgctcgggg agcaggaggt
    11941 ggtgctcacg gtgccggctt cattcgacgc ggctgcccga gacttgacgg tcgaggccgc
    12001 gtacgcggca ggtctcgaga acgtcacgct actcgaggag cctcaggcgg ctctgtacgc
    12061 ctggatcgaa gctcacggcg aggctttccg cacgcatttg aagccactcg acgtggtgct
    12121 ggtgatcgac atcggcggcg gaacgaccga tttttcggcg attgctttgc tcgagaatca
    12181 aggctcgctc gagctgcacc gtgtggcggt gggcgatcac atcctgctcg gcggggacaa
    12241 catggatttg ttgctcgcgg ccgtggtccg caacaagttg cgcgacgcgg ggacggagct
    12301 ggacgcttgg cagatgacgt cgctctcgca ggcgtgccgc gcggctaaag agcagctatt
    12361 ggccgagaat cctccagaaa gcgtgcccat cgtcattccg agccgcgggt cgcagctgct
    12421 cgggcagtca attcggaccg agctcctgcg gcaggaggtc gagcgcgtga tcttggacgg
    12481 cttctttcct ggtgtggagc tcgcggccaa gcccacccaa cggcaacgcg ccggcctcgc
    12541 tcaactcggc ctgccatatg cttcggatcc cgctatcacc aagcacctcg cgcactttct
    12601 gactcggcaa gccggcgcgc tcgcgaactt gcccggattt cccgcggcaa gcgagctact
    12661 gcggcccact tcggtgctct tcaatggcgg agtggtgaag tcgcaggcca tccgcgggcg
    12721 tttgctcgcg tgtctgaatc aatggttgga ggcctcgggc gctcctccaa ttcgtgtgtt
    12781 gcccggcgaa gatccggatc tggccgtggc tcggggcgcc gcctactacg gcaaagtccg
    12841 gcgcgggcgt gggttgcgca ttcgcggcgg gaccgcccga gcctactatg tcggcatcga
    12901 gagccccatg cccgccatcc ccggcttgga gccgcccatt tcggcgctat gcgtggcacc
    12961 gttcggcatg gaggaaggca gccagacgga gcttccgaaa cacgaactcg gggttatcgt
    13021 cggtgaaccc gtggagtttc gcttcttcgg ctctagcgtg cgtcgcgggg attccgcggg
    13081 tacgttgctc gaacatggga aatccggcga gctcgaggag ctctcgccga tcggcatgac
    13141 cctggtcgcg gagggccgcc aaaaaggcga gttcgtcccc gtgcacctgc gcgcgagcgt
    13201 cacc
//
LOCUS       DASTKU010000140         9195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_26721,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000140 DASTKU010000000
VERSION     DASTKU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9195
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26721"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1457
                     /locus_tag="VFQ61_04955"
     CDS             <1..1457
                     /locus_tag="VFQ61_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9953824.1"
                     /translation="AERMKDEHRGQFAVVTAAASDGTSQPPGGAESRFERTVELETGS
                     HDPTAEETQTLMSRKVRRAELSMMPPALDPTRYVDRGEIGRGGTASVRLVEDQVLWRE
                     VAMKVLLSEYEPEHQKMFRFLREARITAFLDHPNIVPVHELGIEAEQQKLFFTMKRVD
                     GRSLSDVIRDVHANQATPEGLYQLLQIFVKVCDAVGFAHSRGIVHRDLKPANIMVGGF
                     GQVYVMDWGAALLLPDCTLPHDRLEAEDENLTIIGTAAYMAPEQAFGDARRIDQRTDV
                     YGLGSILFQILSGRGPHHDTDSRKALSRARLGQIPNLQELTSPWHELPSQLCEIAERA
                     LAKQPEDRYANVAELEHAVEQFSLGGGWFRSKTFAPGELIVREGEVGDAAYIITSGEC
                     EVYKTALGEKRILGRLGPSDVFGEAAVLTGETRMASVVAVTEVVVRVVTPSSLEHELR
                     QNPVLAAFVKALAARFVSLDQALSRQRGWTHRES"
     gene            complement(1485..1817)
                     /locus_tag="VFQ61_04960"
     CDS             complement(1485..1817)
                     /locus_tag="VFQ61_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953825.1"
                     /translation="MARSFWSRSVLVTWVGLGCLVACTSEDSVQAEFEEFVSAHNSCV
                     SAEDCVAIYPGCPLGCYRYVNKQYERAAIDKAKELVADYERWGRSCEYDCVETRPPEC
                     EANRCSSP"
     gene            complement(1893..2219)
                     /locus_tag="VFQ61_04965"
     CDS             complement(1893..2219)
                     /locus_tag="VFQ61_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953826.1"
                     /translation="MSLAWESVLPITRVSDVLHTSLQLVLNLAFPVYVVRRDLARLGP
                     EQLARSWNEASLWCAAATYGSFCLIVHFTRTRRSIYGFGLGLTWTSLGTALSILVGSL
                     VHMLPG"
     gene            2266..2598
                     /locus_tag="VFQ61_04970"
     CDS             2266..2598
                     /locus_tag="VFQ61_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953827.1"
                     /translation="MQATSDDRDCHSCGGVGELPTDFGPRDCPDCGGAGFLPSRNVLV
                     EWRARDIERALAAGINPAAVDVRWLLAELRNARKALTDVIALAHDVQDGDAIAMRIRI
                     SASRALGL"
     gene            complement(2634..5342)
                     /locus_tag="VFQ61_04975"
     CDS             complement(2634..5342)
                     /locus_tag="VFQ61_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953828.1"
                     /translation="MTRPAPKAFLKRRLATMGFAAVLACVLRLPGHGAPRERSLEELA
                     HMLGKAADVQVSSQSIFWEPAESLVAELWWGRRLLFLGAASDGKHSAPRDVFRARVRL
                     TFDGTPIQVTEIRNLTQTPHADEIGLKGIGSRAAFATVSFGRVQAVSVLETTGVRPTE
                     RPQRWFDQLLLAVSSYQETGTFLGLGRTDIVLDVPAASVELQLTPSRLALEFGDRGRN
                     LTYALDQRSLRAESGADAYGARVLPEVHGAKPLVLWMVDTVREEVGPTPIAWLEKVAF
                     GAKDRFKRTTYSWFAPKQGRALRAANEPARARILEPSALMNAGDTWPPPRVPSLWKST
                     EPGEGEWAPVAYGFLKPMRGTSAGEKPPPYFYRTYIRPDRERPYSKVHLIAMDMRQLE
                     IGMQAGYEDPKPTAGPPGEGKLPRDPAIYERVVATFNGAFKTTHGSYGMMVQRRVLLP
                     PVKGGASVIVNDAHEVGLGSWPQSDQIPADVVSFRQNLDPLLEDGVPNPTGRLLWGWQ
                     LEGTSVMTQRTGLCVTPAGHLYYAFGTELDGKTLGQALYQAGCSYAIHLDMNPAHCGF
                     VFSDIRNPRTNDMTLRLASDDMRIAPDKYARWSAKDFFYVMVRDAVPHDGSGLTWTQD
                     EGTQPPPSFLPGVFRSKLGLGKLQIELLSVEKGRADFRVRAGAREPARSTQGSGLSSE
                     EEARVLGALGLGHSTDAVRLGLVLGENVLIPLRSGYATLRLSQASGPQLLLPSATPAL
                     LGKEEQAIQLPLLAEGGQLLERAHERGALRARAALCVSPAGRLLVARAEHDSSDALAE
                     ALIRSGCENVAELDRGSHYPAFLHRAGTSTPPVDGYEASVLYVLSRNMVPHAFRWKPD
                     GSVPSTMVTSYDVPHPGANVTASNTKRKRKRPETNGESGESRGARP"
     gene            5469..6290
                     /locus_tag="VFQ61_04980"
     CDS             5469..6290
                     /locus_tag="VFQ61_04980"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET9953829.1"
                     /translation="MAASNLVQRLLTAAVAVPLLLWLLYGGPAWGFFALVALVSLAGA
                     LELFRMTHAGDVFGQGYGMAATFAVLVTLYVFSQRPDLLMGVLLVVPLLGILMPLVRL
                     GDIKTAGLRMMGNVAGPLYLAGPLAALTLLRRDHGTAGPSWVLLALMFAWFADTGGYF
                     FGRFLGKTKLYEAVSPKKTRAGFVGALVGATLGALLAHFWYLPELPLLEGVLLAVLSG
                     AFGQFGDLAESLLKRSIGVKDSGNIVPGHGGILDRIDALLVVAPIVYLYTSMRGL"
     gene            6481..7821
                     /locus_tag="VFQ61_04985"
     CDS             6481..7821
                     /locus_tag="VFQ61_04985"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET9953830.1"
                     /translation="MNDAAPNSSSQGKPSDSPVVGSSLVPPAHRYEFFKVIQQYLDSA
                     AKLASVPDHVRLILSQPKSEIIVHFPVRMDDGKYQIFKGYRIQHNNILGPYKGGMRFH
                     ADASLDDFKALAAMMTWKCALMGVPFGGGKGGIKFNPRAVSRGELSRVTRRFFHALGS
                     NIGPDYDIPAPDVGTNAQTMAWALDTYMNTVGTVSKGATLGVVTGKPISCGGTLGREK
                     ATGQGLVHAVRTWAQRNKFDLQGKKLILQGYGNVGSHTAVLLGQLGVSLIAVGDHTGY
                     LYNPEGFNEYRLKQYVEQHGSIAGYPNGSSITREEFFGIQADLFVPAALENQVGAAEA
                     EALQVRLVAEGANGPCSPEGEQVLRRRGIDILPDVLANAGGVTVSYYEWVQNKRSESW
                     TLEEVDDRLERAMVDAYDRMEHFANTHDCDFRLACYGVALERLTRAYLDREIFP"
     gene            8159..8869
                     /gene="nrfH"
                     /locus_tag="VFQ61_04990"
     CDS             8159..8869
                     /gene="nrfH"
                     /locus_tag="VFQ61_04990"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HET9953831.1"
                     /translation="MSSDEDNPGNSGSGVAPARPVRSPEPATSGKRPFTLPTGPLLLS
                     IALGILAGIGSYTFSYAKGLSYLSTDPRACVNCHIMQREYDGWQKASHHTSAVCVDCH
                     LPVEFIPKYVAKTENGWRHGKLFTTGGFVEPIEIKPAGLVILQQNCIRCHASLTADMR
                     AHGSLGTAQDKAGVPCLHCHANVGHGDRAGLGGPIGEAEKHELQQLSNKASAHAPAPG
                     PASGAVAPAPPASAAPSH"
     gene            8909..>9195
                     /locus_tag="VFQ61_04995"
     CDS             8909..>9195
                     /locus_tag="VFQ61_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953832.1"
                     /translation="MNDQTNKTPWYQTRAAYGALTAVIAAGAAGVTALLMNISERKAE
                     QRAPARLVEVTENDTSPEKWARNWPAQYDGYKKTALKTSTRFGGHAGSEALP"
BASE COUNT         1652 a         2831 c         2979 g         1733 t
ORIGIN      
        1 gagctgagcg catgaaggac gaacatcgcg gtcagttcgc ggtcgtcacg gcggctgcta
       61 gtgacggaac gagccaaccg ccaggcgggg cggagagccg tttcgagcgg acggtggagc
      121 tcgaaacggg aagtcacgac ccgactgccg aggaaactca aacgctgatg agccgcaagg
      181 tgcggcgggc agagctcagt atgatgccgc cagcgttgga ccccactcgc tacgtcgatc
      241 ggggggagat tggccgcggc ggcacggctt ctgtgcgcct ggtcgaagac caagtgctgt
      301 ggcgcgaagt cgcgatgaag gtgttgctct ccgagtacga gcccgaacac cagaagatgt
      361 tccggttttt acgcgaagca cgcattacgg ccttcttgga tcacccgaac atcgtgccgg
      421 tgcacgagct gggtatcgaa gccgaacagc agaagttgtt cttcacgatg aagcgcgtcg
      481 acggccgttc cctgagtgac gtgatccgcg acgtccacgc gaaccaggct acacccgagg
      541 ggctgtatca actgctgcag atcttcgtga aggtgtgcga cgccgttggg ttcgcgcaca
      601 gccgcggcat cgtgcatcgc gatttgaagc cagccaacat catggtgggc gggttcggtc
      661 aggtctacgt catggattgg ggagcggcgc tgctgctgcc ggattgcacg ctgcctcacg
      721 accggctcga ggccgaagac gagaatctga ccatcatcgg aaccgccgcg tacatggcgc
      781 ccgagcaggc ttttggtgac gctcggcgca tcgatcagcg caccgacgtg tatggcctcg
      841 gctcgatcct ctttcagatc ttgagcgggc gcgggcccca ccacgatacg gactcacgca
      901 aggcgctgtc acgcgcacgg ctggggcaaa ttcccaatct gcaggagctc acgagccctt
      961 ggcacgagtt gccgagtcag ctctgtgaga ttgcggaacg cgcgctcgct aagcagcccg
     1021 aagatcgcta cgccaacgtc gccgagctcg agcacgccgt ggagcagttc tcgctcgggg
     1081 gcggttggtt caggagcaag accttcgctc ccggagagct gatcgtgcgg gaaggcgagg
     1141 tgggagacgc tgcgtacatc attacgtccg gggaatgcga ggtgtacaag accgcgctgg
     1201 gggagaaacg catcttgggt cgactggggc cgtcagatgt attcggtgag gccgcggtgc
     1261 tcacgggaga gacgcgcatg gcgagcgtcg tggccgtgac cgaagtcgtg gtgcgcgtcg
     1321 tgacaccgag ttcgttggag cacgaacttc gccagaaccc ggtattggcc gcgttcgtca
     1381 aagcgctcgc ggcgcgcttc gtctcgctgg accaagcact ctcgcgccag cgggggtgga
     1441 cccaccgcga gtcctgaggc cgtgcgggcc gccaaaacga agattcaagg cgacgagcaa
     1501 cgattggcct cgcactcggg cggcctggtc tcgacgcaat cgtactcgca gcttcggccc
     1561 cagcgctcgt agtccgcgac caactctttg gctttgtcga tggcggcgcg ctcgtattgc
     1621 ttattcacgt agcgatagca gcccagcggg caaccgggat agatggccac gcagtcctca
     1681 gcgctgacgc acgagttgtg ggcggacacg aactcttcga actcggcttg cacgctgtct
     1741 tcactcgtac acgccaccag acagcccagc ccgacccatg tcaccagtac cgatctcgac
     1801 cagaacgagc gagccatgca cccgagccta ccacgatctt cagacagtcc ccggggcggc
     1861 ttggctcgaa tcccccaacg tcgggagccg cttcaacctg gcaacatgtg gacgagtgac
     1921 cccaccaaaa tcgacaaggc cgtgccgaga ctcgtccatg tcagccccaa gccgaacccg
     1981 tagatgctgc gccgcgtccg cgtgaaatga acgatcaagc agaacgagcc atacgtcgct
     2041 gcggcacacc acaggcttgc ttcgttccaa gaccgagcca gctgctcggg gccgagccgc
     2101 gccaagtctc gccgaactac gtataccggg aacgccagat tcagcactaa ctggagcgac
     2161 gtgtgcagca cgtccgacac gcgggtaatc ggtagcacag actcccacgc caaagacatg
     2221 cgattcgcga aagcctccga cgtttgactg tgctaaccgc cgggaatgca agcaaccagc
     2281 gacgaccggg attgccacag ctgcggcggg gtgggcgagc tgccgaccga ttttgggccg
     2341 cgtgattgtc cggattgcgg tggtgcgggc ttcttgcctt cgcgtaacgt tctcgtcgag
     2401 tggcgtgccc gcgacatcga gcgggcgctt gcggccggca tcaacccggc ggcggtcgac
     2461 gtgcgctggt tgctcgccga gctacgcaat gcgcggaaag ctttgaccga cgtgattgct
     2521 ttggcgcacg acgtacagga tggagacgcc atcgccatgc gcattcgcat ttccgcgtcg
     2581 cgcgcgctcg ggctttgaat tttttctgtt gtccctagct cacatgcgct gcgctagggg
     2641 cgagcgccgc gcgattcgcc agactctcca ttcgtttcag ggcgcttgcg cttccgtttg
     2701 gtgttgctgg ccgtgacgtt ggctccgggg tggggcacgt cgtagctggt gaccatcgtg
     2761 ctcggtacgc tgccgtcggg tttccagcgg aaagcatggg gcaccatgtt gcggctgagc
     2821 acgtacaaca cgctcgcctc gtacccatct acgggcggag tgctcgtgcc ggctcgatga
     2881 agaaacgcgg gatagtgtga acctcggtcg agctcagcca cgttttcaca accggagcgg
     2941 atgagtgctt ctgccaaggc atccgagctg tcgtgttcgg cgcgggcgac caggagtcga
     3001 cctgcagggc tcacgcagag agcggcgcga gcgcgcagcg ctccgcgctc atgggcgcgc
     3061 tccagcagct ggccgccttc ggccaacaga ggcagctgaa tggcttgttc ctctttgccg
     3121 agcagggctg gtgtcgcgga gggcaggagc agctgggggc cactcgcttg actcaggcgc
     3181 agagtggcat agccggagcg gagcggaatg agcacgttct ctccgagcac caagcccaag
     3241 cgcaccgcgt ccgtgctgtg cccgagcccg agtgcgccca gcacgcgagc ttcttcctcg
     3301 ctcgagagtc cgctgccttg ggtgctgcgg gccggctctc gcgcgccagc tcgcaccctg
     3361 aagtcagctc gccccttctc cacgctcaag agctcgattt gcagcttacc gagccccaac
     3421 ttcgatcgaa atacacccgg cagaaagctt ggcggcggtt gcgtgccttc gtcttgggtc
     3481 caagtcagac ctgagccgtc gtgcggcacc gcatcccgca ccatcacgta gaaaaagtcc
     3541 ttcgcggacc agcgcgcgta cttgtctggc gcgattcgca tgtcgtcgct ggccaggcgc
     3601 agggtcatgt cgttggtgcg cgggtttcga atgtcggaga acacgaagcc gcagtgcgcc
     3661 gggttcatgt ccaggtgaat cgcgtacgaa catcccgcct gatacaacgc ttgcccgagg
     3721 gttttgccat cgagctcggt gccgaaagcg tagtacaggt gacccgcggg cgtgacgcaa
     3781 agccccgtgc gttgggtcat tacactggtg ccctcgagtt gccagcccca gagcaagcga
     3841 ccggtggggt tgggcactcc gtcttcgagc agggggtcca agttctgtcg gaacgaaacc
     3901 acgtcggcgg ggatctggtc gctctgcggc caactgccga gccccacctc gtgggcgtcg
     3961 ttcacgatca ccgaagcccc tcctttgacc ggtggcagca acacgcgacg ttgcaccatc
     4021 atgccgtagg agccgtgggt cgtcttgaat gcaccgttga acgtggcgac cacacgctcg
     4081 taaatggcgg ggtcacgcgg gagcttgcct tcgcctgggg ggcccgccgt aggctttgga
     4141 tcttcgtagc ccgcctgcat gccgatctcg agctggcgca tgtccatcgc gatgaggtgc
     4201 accttggaat agggccgctc gcgatctggg cggatgtacg tgcgataaaa gtacggaggc
     4261 ggcttctccc ccgcgctggt tccgcgcatc ggcttcaaga agccgtaagc tacgggtgcc
     4321 cattctccct ctccgggttc cgtacttttc cagagtgaag ggacacgcgg gggcggccag
     4381 gtgtcgcccg cgttcatgag cgcggacggc tccaagatgc gggctctcgc gggctcgttc
     4441 gcggcgcgca gggctcgacc ctgcttgggc gcaaaccaac tgtacgtcgt gcgcttgaag
     4501 cggtccttgg caccgaacgc caccttctcg agccaggcga ttggggtcgg ccctacctcc
     4561 tcgcgcaccg tatctaccat ccaaagcacg agcggcttcg caccgtgcac ctcgggcagc
     4621 acgcgcgctc cataggcgtc ggcgccgctc tccgcgcgaa gtgagcgctg gtccagagcg
     4681 taggtcaggt tgcgcccacg atccccgaac tccaaggcca ggcggctcgg tgtgagctga
     4741 agctctacgc tcgccgcggg tacatcgagc acgatgtcgg ttcgccccaa gcccaagaac
     4801 gtgcccgttt cttgatatga gctcacggcc aagagcagct ggtcgaacca gcgctgcggg
     4861 cgctccgtcg ggcgcacgcc ggtcgtctcc agcacgctca cggcttgcac cctcccaaac
     4921 gacacggtcg cgaaggcggc gcgactcccg atgcccttca agccaatctc atcggcgtga
     4981 ggcgtctgcg tcagattgcg gatctcggtg acctggatcg gcgttccgtc gaacgtaagc
     5041 cggactcgtg cgcggaagac gtcgcgtggc gcgctgtgct ttccgtcaga cgccgccccc
     5101 aagaacaaca agcgacgtcc ccaccaaagt tcggcaacga ggctttcggc gggctcccaa
     5161 aagatggatt ggctcgagac ctggacgtcg gcggctttgc ccagcatatg cgcgagttct
     5221 tcgagtgaac gctctcgagg ggcgccgtgg ccgggcagcc gaagcacgca cgccagaact
     5281 gcggcgaagc ccatcgtggc aaggcggcgc ttcaggaaag ctttgggggc aggccgggtc
     5341 acgtcggccg gtcctagcga ggcttccgct caagcgcaag tcggccacgt cgggaccacc
     5401 ggtagctggg ccgagacctg ttgaatcata ggcttgagct cgtgtgcgcc tgctggtacg
     5461 ggtcccgcgt ggccgctagc aatttggtcc aacgcctgct caccgcggcc gtagcagtgc
     5521 cgctgttgtt atggctgctc tacggcggcc ccgcttgggg gttctttgca ctcgtcgcgc
     5581 tcgtgtcact cgccggcgcg ctcgaactct tccgaatgac gcatgcgggc gatgttttcg
     5641 gccaaggcta cggcatggcc gcgacatttg ccgtattggt gacgctgtac gtcttttctc
     5701 agcggcccga tctgctgatg ggtgtgctgc tcgtggttcc gctcttgggc attctgatgc
     5761 cgctcgttcg gttaggggat atcaagaccg cggggctgag gatgatgggc aatgttgcgg
     5821 gcccgctcta tttggcaggg ccgttggccg cgctcactct gttgcggagg gaccacggca
     5881 cagcgggacc gagctgggtg cttctggcgc tgatgttcgc ctggttcgct gacacgggtg
     5941 gctacttttt cgggcgtttt ctgggaaaaa ccaagctcta cgaagcggtg agccccaaaa
     6001 agacgcgtgc ggggttcgtc ggcgcgttgg tgggtgccac actgggcgca ttgttggcgc
     6061 acttctggta tctacccgag ttaccgctgc tcgagggcgt gttgctagcg gtcctttccg
     6121 gcgctttcgg ccaattcggc gatcttgccg agtcgctatt gaagcgatcc atcggggtga
     6181 aagactccgg gaatatcgtg ccgggtcatg gcggcatcct ggaccgcatc gacgccctgc
     6241 tggtggtggc cccgatcgtc tacctatata cgtcgatgcg cggtttgtga gcgcgaccgc
     6301 tggttgcgag tgccttcgcc gcggcgggtt ggctgattcg cttcgctggc tgattcgctt
     6361 cgttggctga ttcggtccgc tgggtctggc tgattcgctc ggcgaacctc atgctcgagg
     6421 ggtggccttt tctgactttg ccacgtggac gttgctccat aagatcgtcc tggagaaatc
     6481 atgaacgacg cggccccgaa ttcgagctca caaggtaagc ctagcgattc accggtggtt
     6541 ggcagcagcc tggtgcctcc agcgcaccgt tacgagttct tcaaggtcat ccagcagtac
     6601 ttggactccg ctgcgaagct agcttccgta cccgatcacg tacggttgat tttgagccag
     6661 cccaagtccg agattatcgt gcacttcccg gtgcgaatgg atgacggcaa gtaccagatc
     6721 ttcaagggct accgcatcca gcacaacaac atcctggggc cgtacaaggg aggcatgcgc
     6781 tttcacgccg acgcgtctct agacgacttc aaggccctcg cggcgatgat gacctggaag
     6841 tgcgctttga tgggtgtgcc gttcggcggc ggcaaaggcg gcatcaaatt caatccgcgc
     6901 gcggtgtcac gcggtgaact cagtcgggtg acgcgacggt tcttccacgc gctcggctcc
     6961 aacatcggcc ccgactacga tatccccgcg ccggacgtgg gcacgaatgc ccaaaccatg
     7021 gcttgggcgc tcgacaccta catgaatacc gtgggcacgg tgagcaaggg cgcaactctg
     7081 ggggtcgtta ccggcaagcc aatcagctgc ggcggcacgc tgggtcgtga gaaagcaacg
     7141 ggccaaggct tggtgcacgc ggtgcgcact tgggcgcaac gcaacaagtt cgacttgcag
     7201 ggcaagaagc tgattctgca gggctatggc aatgtgggtt cgcacaccgc ggtcttactg
     7261 gggcagctgg gcgtgtcttt gatcgcggtc ggcgatcaca cggggtactt atacaatccc
     7321 gaaggcttca acgagtatcg tttgaagcaa tacgtcgagc agcacggcag catcgccggg
     7381 tatccgaacg gcagcagcat cacgcgcgaa gaattcttcg gcattcaggc ggacttgttc
     7441 gtgcccgccg cgctcgagaa tcaggttggc gcggcggagg cagaggcgct gcaggtgcgt
     7501 ctagtggctg agggcgcgaa cgggccttgc tcacccgagg gcgaacaagt tctgcggcgg
     7561 cgcggcatcg acatcttgcc ggatgtgctc gcgaacgccg gcggtgtgac ggtgagctac
     7621 tacgagtggg tccaaaacaa gcgcagtgaa agctggacgc tcgaagaggt ggatgaccgg
     7681 ctcgaacgcg cgatggtcga tgcctacgat cgcatggagc atttcgccaa cacacatgac
     7741 tgcgatttcc gcctcgcttg ctacggcgtt gcgctcgaac gactgactcg cgcctatctg
     7801 gatcgcgaaa tctttcccta atgcgcctct aatccggtaa tttgtaggcc gatttggagt
     7861 tcggggccga aatggcccac ttctttgctg tatccggggg cagcagaact aaggagggca
     7921 ggctgcctct gcggcgatcc ccgtacgtaa gcgtgtcggg gatcgtcagc gcagctggtt
     7981 tgtagggcgc tttgcatggc gaagatcatg ctggaagatt tctggctttc tgcgtcgttt
     8041 cagattcata tacaacgcta cgacgcgctt ccgtcgtcga gcgctccgga gcccgcgcag
     8101 ggctgcccca gagtgtctgc tcggcgttgc gctggctagc aatggatcag gccgcttcat
     8161 gagttcagat gaggacaatc cagggaactc aggctccggt gtggcgccag cgcgtccggt
     8221 gaggtcgccc gagccggcta cctcgggcaa gcgcccgttc acgctgccga cgggaccgct
     8281 tctgctttcg atcgctctag gcattctcgc cggaatcgga agctacacgt tcagttatgc
     8341 gaaggggctc tcttacctct ccacggatcc gcgagcctgc gtgaactgcc acatcatgca
     8401 gcgcgaatac gatggttggc agaaggcgag tcatcacacg tcggccgtat gtgtggactg
     8461 ccacctacca gtcgagttca tcccgaagta cgtagcgaag accgagaacg gttggcgcca
     8521 cggaaagttg ttcaccacgg gtggcttcgt ggagccgatc gaaatcaagc ccgcggggtt
     8581 ggtgatcctg cagcagaact gcatccgctg tcacgcatcg ctgactgcgg acatgcgcgc
     8641 gcacggttca ctcggcacgg cgcaagacaa ggcaggggta ccttgcctgc actgccatgc
     8701 caacgtgggg cacggtgatc gcgcgggctt gggcggcccg attggcgaag ccgaaaaaca
     8761 cgagctccag cagctctcga acaaggcttc ggcccacgct cccgcgcccg gacccgcgag
     8821 cggagccgtt gcgcccgcgc ctcccgccag cgccgccccc agtcactgat tttcgcgcca
     8881 aacccttcgt agccttggaa gcgtcttcat gaacgaccaa acgaacaaaa ccccttggta
     8941 ccaaacgcgc gcggcgtatg gagcgctcac ggccgtgatc gcggccggcg cggcgggcgt
     9001 gaccgcgttg ctcatgaata tctccgagcg caaggcggag cagcgagcac cagcgcgctt
     9061 ggtcgaagtc accgaaaacg ataccagtcc cgagaagtgg gcgcgcaatt ggccagcgca
     9121 atacgacggt tacaagaaga ccgcgctcaa gacctcgacg cgcttcggtg ggcatgccgg
     9181 cagcgaggct ttgcc
//
LOCUS       DASTKU010000141         4328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1811224, whole genome shotgun sequence.
ACCESSION   DASTKU010000141 DASTKU010000000
VERSION     DASTKU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811224"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VFQ61_05000"
     CDS             <1..712
                     /locus_tag="VFQ61_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953833.1"
                     /translation="LPPVADAAPVVEHAPARPATSAPKAEAAKVAANPEQDKLLGLAF
                     LGDPKALEQLEARPEASRSAREWLSIAAGREKAGRALEALAAYQRALEREPTLGEDQA
                     LRKFVWEMSHKPEAGETAMRLMAEYFGSKGADMLYRSWVESREVTPISQLARELVYTK
                     RVRDAATPMLAFVLDWREAVSCEDYAAILPRAQLNADQRALMVLQRALRKNSCKLPEE
                     TLRAAIVVAKERPEPVPW"
     gene            945..1097
                     /locus_tag="VFQ61_05005"
     CDS             945..1097
                     /locus_tag="VFQ61_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953834.1"
                     /translation="MGRCTRIGYEAQPSQGERARNREAFMVETERRKSGGRVEKDGVL
                     TWGDLA"
     gene            1325..2593
                     /gene="ltrA"
                     /locus_tag="VFQ61_05010"
     CDS             1325..2593
                     /gene="ltrA"
                     /locus_tag="VFQ61_05010"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET9953835.1"
                     /translation="MAENRTERSATRDLLERVLERSNLQEALKRVKKNKGSPGIDGMT
                     VGQLPGYLKAHWTEIREQILTNRYQPSMVRRQEIPKSGGGVRQLGIPTVLDRLIQQAL
                     LQVMQPIFDPSFSPSSYGFRPGRSALDAVKASKQYVEQGRRYVVDVDLSKFFDRVNHD
                     ILMGRLAKRIEDKRVLTLIRRYLEAGMMAGGVVIERREGTPQGGPLSPLLANILLDEV
                     DKELEKRGHAFARYADDCNVYLRSRRAGERVMTLLRKLFAKLRLVVNEEKSAVDRVWY
                     RDFLGYGLWWNRHGKVQHRVSRKALQAFKTRVRETTSRTRGRSMSHVAEELRSYLLGW
                     KGYFQLSQTRLVFRELDKWIRHRLRAYQLHLWRRGPVIHRELLARGAPRDVAAKVAAN
                     SQRWWRNAQYVANIAFPNRYFIELGIPQLE"
     gene            2891..3598
                     /locus_tag="VFQ61_05015"
     CDS             2891..3598
                     /locus_tag="VFQ61_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953836.1"
                     /translation="MRARNTPGVDASVDEAFTNSTWFVALPVFGVASFCVLYALASSV
                     YAGVSETGYGHLTNYWCDLLAATSPAGHGNPARPFAVLATFVLPLSLVPLWYHLPRLF
                     AAGARACVAVQVTGCIAMVSATLVWTPMHDLAINVVAVFGFCALLMTLFNIDRRRHRA
                     VGMSVRWAGALVLTNYVLWATGTLVWVIPAVQKAAFLGLFIWVAIATRAISRSLRWER
                     EVMDPSAHRLYESSDAA"
     gene            3934..>4328
                     /locus_tag="VFQ61_05020"
     CDS             3934..>4328
                     /locus_tag="VFQ61_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953837.1"
                     /translation="MTNERTIDWARALARMSTAEARSGRGSCSRQRWTALAVIAAALS
                     GCSASEQGGSDSPAAGSQGVETSTGGEASTGPIDTSAPPVDPTDTSGEAETTSGEAET
                     TSGEDAPVVVVEPGKPTAVGEEWLRENTLS"
BASE COUNT          860 a         1248 c         1489 g          731 t
ORIGIN      
        1 gctgccgccg gttgcggacg cggcacccgt cgtcgaacat gctccggcgc ggcccgcgac
       61 ctcggcgccc aaagccgagg cggccaaggt cgccgctaac cccgagcaag acaagttgct
      121 cggtctggcc ttcctcggag atccgaaagc gctcgaacaa ctcgaggcgc ggccagaagc
      181 gtcgcgttcg gcgcgcgaat ggctgtccat cgccgccggt cgcgagaagg cggggcgtgc
      241 tctcgaagcg ctcgcggctt accagagagc gctcgaacgc gaaccgacgc tcggtgaaga
      301 tcaagcactt cgcaagttcg tttgggagat gagccacaag cccgaggccg gcgaaacagc
      361 gatgcgcctg atggcggagt atttcggctc caagggcgcc gacatgttgt accgctcctg
      421 ggtggagtcg cgcgaagtga cgcccatatc acagctcgcg cgcgagctcg tttacacgaa
      481 gcgcgtgcgc gacgcggcca ccccgatgtt ggcgttcgtg ctcgactggc gcgaggcggt
      541 aagttgcgag gactacgcgg cgatcttgcc gcgggcgcag ctgaacgccg atcagcgagc
      601 gttgatggtg ctgcaacgcg cgctgcgcaa gaactcctgc aagttgcccg aagagacgct
      661 gcgcgccgcc atcgtggtgg ccaaagagcg ccccgaaccc gtgccgtggt gagggagcgc
      721 ggcggaggcc aatcacgtga ccacgcggcc cagtccgcgg acccgacgac gctcgagcga
      781 aaaagccgcg cctgcggcaa taggtgcccg cgcgggttcg cgtgcgccca tcatgggcgc
      841 catctttggg gtgcaagtcc ccacgaaagg agatcatgac aatcgaagcg aagcgcaact
      901 gcgggagggt gaccaaccgt ggagaggaag cgcggagcga atccatgggc cgatgtacaa
      961 gaatcggata cgaggcgcag ccgagtcagg gcgagcgggc gcgaaaccgc gaagccttca
     1021 tggtcgagac ggagcggcgt aaatccggcg gtcgtgtgga gaaggatggc gttcttacct
     1081 ggggagatct tgcctagcgc ccgaaagggc gacgcctgga ctggagtccc ccccaggcgg
     1141 agcaagaagt cagcagaggt cgtagtaggt gccgctgtga agtggcgccg aaggaccaaa
     1201 cgaagaggag agcgaaacga cgatgactct cagaggtaaa cggccacaga tgtccgggag
     1261 accggagccc gctgggaaag caaaaggtga aacccggagc gaaaccaggg gcgtggaaga
     1321 accgatggcg gagaaccgaa ccgagcgctc agccacacgc gacttgctgg agagagtgct
     1381 ggagcgctcg aacctgcagg aagcgttgaa gagagtgaag aagaacaagg gaagtcccgg
     1441 gatagacggg atgacggtgg gacagttgcc ggggtacttg aaagcccact ggaccgagat
     1501 acgtgagcag atcctcacga acaggtatca gccctcgatg gtgcgacggc aggagatccc
     1561 gaagagcgga ggaggagtgc gccagttggg cattccgacg gtgttggatc gcctgatcca
     1621 gcaggccctg ctgcaggtaa tgcaaccgat attcgacccg agcttctcac ccagcagcta
     1681 cggatttcga cctggacgta gtgcactaga tgcggtcaaa gcgtcgaagc aatacgtcga
     1741 acaagggcgg aggtacgtag tggacgtgga cctgtcgaag tttttcgatc gggtaaatca
     1801 tgacattctg atggggcgac tggcgaagag aatcgaagac aagcgggtgc tgacgttgat
     1861 acgtcgatac ctcgaagcgg gtatgatggc cggcggagtg gtcatcgagc gacgagaagg
     1921 gacgccgcaa ggcggaccgt tatcgccgct gttggcgaac attctcctcg acgaagtgga
     1981 caaggagctg gagaaacgag gtcatgcgtt tgcccgatac gcggacgact gcaacgtgta
     2041 cctgcggtcg cggcgagcgg gagaacgcgt gatgacgcta ctgcggaagc tctttgcaaa
     2101 gcttcggctc gtggttaacg aggagaagag cgcggttgac cgagtgtggt atcgggactt
     2161 cctgggttac ggcctgtggt ggaaccggca cggcaaggtc caacaccgcg tgagtaggaa
     2221 ggccttgcag gccttcaaga cgagggtcag agagacaacg agtcggaccc ggggacggag
     2281 tatgagtcat gtcgcggaag aactcaggag ctacctgcta ggatggaagg gttacttcca
     2341 gctgtcgcag acgcgcctgg tctttcgtga gctcgacaag tggatccgcc atcgactcag
     2401 agcgtaccag ctccatctct ggcgacgcgg cccggtgata caccgcgagc ttttggctcg
     2461 cggtgctcca cgggacgtcg ctgccaaggt tgccgcaaac agtcagcgct ggtggcgcaa
     2521 cgcacaatac gtggccaaca tcgcctttcc caaccgctac ttcatcgaat tgggcatccc
     2581 ccaactcgag tgagctcaac ttttcgaacc gcccggtgcg gacccgcatg ccgggtggtg
     2641 tggcaggggt cgtcggggat ttcccgacgc ccctatgccg attggggccc gtcatcgcgg
     2701 cccgcggctg ccgttgcggc gcttcgtgcg tgtcaatttg ccgtaagact gcacaatccg
     2761 gcacggcgcc gtaccagcgc gctccgccat cgcgcccgtg ctcccggtcg caggcgaaca
     2821 ccagcgacac ggagttgccg agccgctacc gttggctccg gcaacagcgg ttccaattgg
     2881 agagcgagtc atgcgcgccc gaaacacacc cggtgtggat gcaagcgtgg acgaggcgtt
     2941 cacgaactcg acctggttcg tcgcgctgcc tgtttttggt gtcgcctcgt tctgtgtgct
     3001 ctatgcactc gcgagctccg tttacgcggg ggtcagcgaa acggggtacg gtcacctcac
     3061 caactactgg tgtgatctgc tagccgcgac atcgcccgct ggccacggca accctgctcg
     3121 tccatttgct gtgctcgcca ccttcgtgct ccccttgtcg ctggtgccgc tttggtacca
     3181 cttgccgcga ctgtttgcgg ccggggctcg cgcctgtgta gcggtgcaag taaccgggtg
     3241 catcgcgatg gtcagcgcga cgttggtctg gacgccgatg cacgacttgg ccatcaacgt
     3301 cgtcgccgtg tttggcttct gcgcgttgct gatgaccttg ttcaacatcg atcggcgcag
     3361 gcaccgggcc gtcggcatgt ccgttcggtg ggcgggagca ttggtactca cgaactacgt
     3421 gttgtgggcc accgggacgc tggtttgggt aatccctgcg gtccagaagg cagcctttct
     3481 cgggctgttc atttgggtgg ccatcgcgac gcgcgcgatc tcgcgatcct tgcggtggga
     3541 gcgcgaggtg atggacccta gcgcgcatcg tctatacgaa tcgagcgacg cagcgtgagc
     3601 gagggtttgt gttaggttta tgcaaggcga gacgcagcgc cggcccctgc gactgacgcg
     3661 cgagtccagc gttgggtgta tactggaagt gcagggatcc cacgctcggt ggcacagctg
     3721 gcacaactat cggtgacgat ccatgtccgc cctgtcagtg cagagacgaa atcgaagggg
     3781 ggtgaacggt gtatactgcg tgttaaagtc cgtgggccga tcgcgacacc agccaaaaag
     3841 cgcggcgcgt gttagtcagg ggccatcggt cgacagcgtt ttgcagattg tgccttgtgt
     3901 cgttggtccg gtgattgcac cctgtggagc accatgacca acgagagaac gattgattgg
     3961 gcgagagccc tggcgcggat gtcgaccgcc gaggcccgaa gcggccgtgg ctcgtgttcc
     4021 cgacagcgct ggactgcact cgccgtcatc gcggcagcgc tcagcgggtg ctcggcgagt
     4081 gaacaagggg gtagcgattc gcccgccgcc ggttcgcagg gagtcgagac gagcacaggc
     4141 ggggaagcct cgacgggtcc gatcgacacg agcgctccgc ccgtggaccc gactgacacc
     4201 agcggcgagg ccgaaaccac cagcggcgag gccgaaacca ccagcggcga ggacgcgcca
     4261 gtcgtggtcg tcgaaccagg aaagccgact gccgtcgggg aggagtggtt gcgagagaac
     4321 acgctttc
//
LOCUS       DASTKU010000142        24619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1365264, whole genome shotgun sequence.
ACCESSION   DASTKU010000142 DASTKU010000000
VERSION     DASTKU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 24619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24619
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365264"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFQ61_05025"
     CDS             <1..354
                     /locus_tag="VFQ61_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9953838.1"
                     /translation="TLLQSEGVLVSAFASGRDALEAVDQIQPRLIVSDIGLPDYDGYQ
                     FMSDLRSRPDGRGGNTPAIALSAAPADRDRSRALLAGYQWHITKPVHHEELVDAIRCL
                     LRVRPIETSETKRAE"
     gene            773..1552
                     /locus_tag="VFQ61_05030"
     CDS             773..1552
                     /locus_tag="VFQ61_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9953839.1"
                     /translation="MDALRIMTYNVHSCRGVDGRTRPERILKVIRKSNPDVVALQELD
                     GPAASHDFAQALGMRHFYVEARSKGSTGFGNALLTRFPADLVRQEALPRLSPQQEPRA
                     AQRLRVHTSFGSVEIVNTHLGLSDAERVLQVDKLLSVDWLAEVTGAQYSVLCGDLNAR
                     PGSPAYRRLTERLRDAQTSLGQGQSTFPSLFPIVRIDHVFTNDLLVARHAEVPVGTLA
                     RLASDHRPLVVDLSPVQTNQAPFARGKSSSSAPLERDVLVE"
     gene            1558..3213
                     /locus_tag="VFQ61_05035"
     CDS             1558..3213
                     /locus_tag="VFQ61_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET9953840.1"
                     /translation="MRPILIPGETCWQECEVDEAGALFDGCDYYRAFYQQALRARGHI
                     LIAGWQFNTNVPLLRGEEAKSAPLPVEFMPFLAELGRRNPNLGIYILAWDYSPVFALE
                     REWLQREAFAFGTPDNVQFCFDDKHPLGASHHQKFVVIDGALAFVGGMDLAQGRWDDR
                     RHLASNPLRWEAGAPQKPYHDSMAYVRGPAAQQLARLFAERWEAATGEPLTLPVTSPR
                     EPNPITGSIPIEAKRVAISRTGHCTTSDASGETSYQEIQSLFLRSIERAEHLIYLETQ
                     YLTSRVFHETLMRRMRDSARPLDVIIVMPDDADTPKERLALGAAQNRILRSLCDTAAR
                     SGSRLRVYCTVPEGTLRDETVPEGAWSEGPPSERGRSDGPRSERPAPETARPEGSAPE
                     PPATFIHSKILLVDDRLICVGSANITNRSFTLDSELSLAWEAENDTDGVARSVVRMRA
                     ELLSEHAGVAWAPEFFRLHGLISRLDAAVASGKTRLKRRRIPSAASEETPLFDLTGLF
                     DPEKPLDELALDQILPGRETDEDGAAGPRSGPISEMQAASERG"
     gene            3224..4180
                     /locus_tag="VFQ61_05040"
     CDS             3224..4180
                     /locus_tag="VFQ61_05040"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9953841.1"
                     /translation="MADVTGAPALDRRKGKRKQWLRWLLSAALLCVAGAFVWRELRGL
                     SVHEIWLHVRERSWAQIGAACALAVVNYLQLTRFDALALRYLNQRLPERRLVLASFIA
                     TAFGHNIGPSVASGGSIRLRFYSAWGIPAWQVATLIAFLSVTFFIGLCAVAGITFVSC
                     SAADAEHLPLPLWLLRTIGGVFLGLLVAYLAVCARGPTLTLRGKRVSPPRIGIALGQI
                     ATGGLDWLMMAAIFSLLLPSHTASYFEVLRSLLVAQVAGVFSQVPGGLGVFESLMLSS
                     LGHIVSAPELLGTLLLYRALYFFLPLLFALLLMLVSEVRRKA"
     gene            complement(4211..5266)
                     /locus_tag="VFQ61_05045"
     CDS             complement(4211..5266)
                     /locus_tag="VFQ61_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953842.1"
                     /translation="MNRRPVKEMRKRGIVLAALAALSFAWSCRGGAEADSGSETHFLR
                     VCPASCNDGLECICGVCTARCSDDAVCRELAGSASCVSLAARIAEGRCQPEARGQVCD
                     ASCLVAADCARLGQNFVCDAGYCRDVPQNQAPAPVAECTPPSYEPDQIVILGDVLIEL
                     TVFGSALAEHAVSAGQLAEGMTFRNYASHLTSTLAEGPFSIGNEYTTALQGEPPKLVV
                     LDGGATDFLNDPCTGMLNDECPAVKAAISGAELLFQRFAQGGVEQIVYFFYGDPVGDP
                     GLKAGLDLLRPLLRNACGRSAVPCHWLDLRELFAPHPEYVGADGLVFSEAGARASAAA
                     VWKVMEDRCLVPRTSDR"
     gene            complement(5247..6350)
                     /locus_tag="VFQ61_05050"
     CDS             complement(5247..6350)
                     /locus_tag="VFQ61_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953843.1"
                     /translation="MTIELLIEAIVRQTTILIAQLATSGGTRAPLAQVANQVFLDLVH
                     ELERQGVSRKVSADMFGLGLRTYQRKIQRYTESSTDRGRSLWVAVLDFVQSSETVTRA
                     QVLEKFAGDDESQVRGVLHDLCESGLVSVSGTGARMKYRLTPEEELEQLRQSNSSEGV
                     DELLWAMIYREGPCSFANLAKKPISERELTESLARLVEAGRVECDTTQGYYSARALVL
                     PLGSPVGWEAAVFDHFKALVNTITCRLSIHRSSPNLADRVGGSTYTLDVWPGHPHEQE
                     ALGTLRDLRQKLGELRDKVEGFNAHNPVPESYTQVLLYAGQCLIQQGASPSRAPASGA
                     ATSSNVSSSNAPVPPTHEDEEPQVSSEDEPTPR"
     gene            complement(6554..8329)
                     /locus_tag="VFQ61_05055"
     CDS             complement(6554..8329)
                     /locus_tag="VFQ61_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9953844.1"
                     /translation="MNDWSAVNAVEAAEESVTDILVVDDNPRNLLAIDAALGDFAGRI
                     VKAKSGEDALRALLKHDYAVILLDVQMPGLDGFETARLIRSRDRCKHTPIIFVTAYDQ
                     KHDEVVRGYQLGAVDFLFKPISPEILKAKVAAFVELRKHVAEIARQAKLLREHERREQ
                     EDRLNEERRRWEADTLRRVAEENKKRAEQLAATVAERERAQAELTRAVRELELADRRK
                     NEFLATLAHELRTPLSPIALGVDLLAELLSDASEVSNVLTAMRRQIRHLVRLVDDLLE
                     VSRISQGKIQLRRAPLSLGDAIERAVELCRPLIEERGHRLQVEECSSPVYVDGDRVRL
                     TQVVTNLLTNAARYTPPQGQIQLKCRKEEGYAVLEVIDNGRGMTPDLVERAFDIFEQG
                     ESSEETSGLGIGLSLVKDLVVLHGGKVEAFSEGVDRGSTFRVRLPLLSQELADAQQRA
                     DNEAPGVRVSGTQRRLKVLLVEDNEDIRELTSAMLRRGGHHVQMAEEGHAAIELASSQ
                     HFDVALIDIGLPGMGGAEVGEALRRILGSRTRLVAVTGYGHAEVRNDAAKAGFDAYLI
                     KPVDHADLDAQLRLAANRLAAAEAS"
     gene            8590..14856
                     /locus_tag="VFQ61_05060"
     CDS             8590..14856
                     /locus_tag="VFQ61_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET9953845.1"
                     /translation="MQNATTNGRKTASTGKTDSLERKAILAGLRALRRGEFGTRLPDG
                     LIGIDGQIAEAFNDIALQLASLEGELQEVRVAVGKEGRNKRRLQRSIGKNGWESIVGS
                     VNELVDDVTTHTAELTNVVEAVTRGDFSVRVDIEGRAEPLRGTALRQAESVNKMVLYL
                     DSYTRDLIRLAIEVGVDGKLGAESRQREVTGVWLELGNAVNLMAANLTAQLREIARVT
                     TAVAQGDLSKTIEIEVRGEILDLKHTINTMVGQLRSFADEVTRVAREVGTEGMLGGQA
                     RVRGVSGVWRDLTESVNFMADNLTTQVRNIAEVSTAIARGDLSRKITVAAKGEIRDLA
                     ATINTMVDQLSAFASEVTRVAREVGTEGVLGGQAQVTGASGVWRELTQNVNSMANNLT
                     SQVRNIAEVVNAIAEGDLGRKITVDARGEILALKNTINATVDKLNRFAAEVTRVARLV
                     GTEGTLGVQAEVRDVSGIWKDLTDSVNHMGRNLTDQVRDIAAVTTAVANGDLSRKITV
                     EVKGEILALKNTINDMVDTLSSFADEVTRMAREVGTEGVLGGQAEVPDVSGIWKELTD
                     NVNSMANNLTNQVRNISEVATAVASGDLSRKITVDARGEVQDLKETLNAMVEQLNSFA
                     AQVNRVAREVGVDGRLGGQAVVEGVRGTWKELTDNVNLMASNLTNQVRDIAEVTTAVA
                     RGDLTRKIAVDARGEILELKNTINTMVDQLGSFADQVIRLAREVGVEGKLGGQADVRG
                     VSGTWRQLTDAVNSMANNLTDQVRNISQVATAIARGDLNRKIVVDARGEILELKNTIN
                     TMVDQLSSFAEEVTRVAREVGTEGRLGGQASVEGVRGTWKELTDNVNQMASNLTNQVR
                     DIAQVATAIARGELSRKVSVEVRGEILELKNIMNTMVDQLSSFADEVTRVAREVGVEG
                     KLGGQARVRGASGIWKELTDNVNLMGSNLTDQVRDIAEVTTAVATGDLSRKITVEVRG
                     EILELKNTINVMVDQLRSFADEVTRVAREVGTEGVLGGQAEVRGVSGTWRELTDNVNF
                     MARNLTAQVRGIARVVTAVARGDLDQELRLEARGEIAELVETINGMTSTLSVFAEQVT
                     GVARDVGVEGRLGGQADVPGAAGVWSDLVSNVNELAGNLTRQVRAIGDVATSVTKGDL
                     SRSITVEARGEVALLKDNINQMIHTLAETTRVNQEQDWLKTNLTRFTRMLQGQRDLLT
                     VAQQVLSELASVVGAQHGAFYMASKDSGETTLQLFASYAFVERKSASNRFRLGEGLVG
                     QSALEKKRILVTPAPTDYVQITSGLGSAPPISIVVVPVLFESDVKGVIELATFERFTP
                     VQVAFLEQLLESLGIVVATIEATMRTDTLLRQSQRLTEELQSQQEELQQTNEELEEKA
                     HQLSEQKAEVERKNREVETAKRELEEKAEQLALTSRYKSEFLANMSHELRTPLNSLLI
                     LSRQLGDNADRNLTEKQVRYAQTIHQSGADLLALINEILDLAKIESGTMAVDASPVRL
                     SELTDYVERTFRHLANEKNLELFTALTEEVPETVETDDMRLRQILRNLLSNAIKFTER
                     GEVSLRVCPAKALPNRPSSVNPDSIAFIVKDTGIGIPRDKQRIIFEAFQQADGSTSRK
                     YGGTGLGLSISRELTALLGGELTVESEPGAGSTFTLYLPRRVPDSSRRNAARSEPLPP
                     VQTKPTPSLPRREGPRIDDDRTNIRPGDRVLLIIEDDQPIAELLLNMARERGFRGVVT
                     MHGQEALEVARSLRPDAITLDLRLPDIDGWVLLDQLKHGPETRHIPVHVISALDGERR
                     ALENGAFAVLRKPATQEALMSALNSLAGFLERRVRHLLVVEDDPVQRQSIVDLIGDGD
                     VVTTAVGSGEEALALANEKNFDCIVVDLRLPGLSGFDLIHQLKQSPNMSRTPIVVYTG
                     RELTRSEDAELNRMAQSVIIKDVRSPERLLEETALFLHRVEANLPEDKRDLLRRVAQM
                     DPLLSGKRVLLVDDDLRNIFALTALLEQHKMLVRSAENGREALELLDKDSVDIVLMDI
                     MMPEMDGYEAIRHIRKNPKHAQLPVIALTAKAMAGDRDACIEAGASDYITKPVDLSQL
                     LSLLRVWLYGRSRA"
     gene            14853..15683
                     /locus_tag="VFQ61_05065"
     CDS             14853..15683
                     /locus_tag="VFQ61_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET9953846.1"
                     /translation="MSLSTAGVEQAEIEALVTAILQRYGYDFSHYARASFVRRVRHAL
                     NEEALPSVSALQAKLLRDPLAMRRFLTYLSVNVTAMFRDAEFYEVLRKQVIPWLRTYP
                     FVRIWHAGCASGEEVYSMCILLEEEGIYDRCRIYATDLSEVVLHRARMATYPLSLVQR
                     YTANYHKAGGELEFSRYYTADQENAIIHERLKKNVVFSLHNLASDGSFNEFHLVCCRN
                     VMIYFDEVLQGRVLTLFDQSLSRGGYLALGRRETLRFSSLAQTYTEIEGNVKIYRKPR
                     "
     gene            15696..16271
                     /locus_tag="VFQ61_05070"
     CDS             15696..16271
                     /locus_tag="VFQ61_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET9953847.1"
                     /translation="MEAIRAIVGIGASLGGLTACRQVLWDLPSNFPAPILIVQHRLAE
                     AEPLLAGLLGKEDGLRVVEPDDKALLSPGTVYVAPSNYHMLVEQGGTSIALSTEGVVR
                     YARPSIDVLFESMAATFGRGAVAVVLTGSSDDGARGAQAVKRAGGRVIVQTPTQAESR
                     IAPAAAIARMKPDAVVDVEAIAPLLRSWLRA"
     gene            complement(16282..17280)
                     /locus_tag="VFQ61_05075"
     CDS             complement(16282..17280)
                     /locus_tag="VFQ61_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET9953848.1"
                     /translation="MKVLEDEDNEPKQPAPGTTSPELTGASGVAPHANEGGSPGSAAP
                     LSGSAAAPAPAPAPPPPEGTPVATRGFKPSSSRVWIELAVLGAVLLALGGSAIWGGGV
                     LAESLTPWVPLSVDRRLGELSEASLGQADCPDAGAKAYVQELARVLTECGVHTPFSFQ
                     FRVADDATVNAFALPGGWVTVNSGLLQAAQSSEEVAGVLAHELQHVVLRHGTRRLLRE
                     MGGSFALSLLLGGSDLKSHAAIGAKVASLRYERDEELAADRAGIELLERCGIEASGLA
                     QFFDRMVEQGRSPPEFFSTHPDPGERARRARSRSTSGNPTPARALPILPKPIRCGP"
     gene            17366..18061
                     /locus_tag="VFQ61_05080"
     CDS             17366..18061
                     /locus_tag="VFQ61_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HET9953849.1"
                     /translation="MNHYVEHGPSFAWLKIQLEAGETLQAEAGAMVAREPHVEMETRL
                     NAGSSPGVFRLIWAVIVAFVRKFLGGETMFVNRFSAPARGEVILAPALSGHVIAQRMR
                     PGERLFVQRGSYLASSGSVDTRVRFTGLRSLLGGEGLVLLECSGEGDLWLNSYGGVLP
                     LPVNGTFTVDTGHIVAFDGTLDFRVKSVGGMKSLLLSGEGLVCEFSGRGTVYIQSRNL
                     QALVGWLSPLLPS"
     gene            18070..18759
                     /locus_tag="VFQ61_05085"
     CDS             18070..18759
                     /locus_tag="VFQ61_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HET9953850.1"
                     /translation="MHIDIRYNPAHSLAMVHLQPGESVRAEASAMVSMTSNIDVRTTA
                     THASGKGLLGGVKRALVGGESFFQNLYTARSSGAHVNLAPRLCGAMVVHELEPHADLF
                     IQGSSYVASAESVQIDTQFQGFRGLFSGESFFFLKVSGRGPVLMNAFGAIEARELDGE
                     LILDTGHLVAFTAGIEYSISKAGAGWISSYLSGEGLVLRLRGRGRLWIQSRNPREFGT
                     LVGRLLPARES"
     gene            18764..19450
                     /locus_tag="VFQ61_05090"
     CDS             18764..19450
                     /locus_tag="VFQ61_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HET9953851.1"
                     /translation="MKHQILDSPDFAMLELVLDAGDSVVAESGAMVAKDSQIELSTNM
                     RGSFLHAAKRKLLGGESLFQNTFRATANGQRLYLAAAAEGDLKAHVLAAGEALFLQSG
                     AYVAHVGSDLVLDTHFAGLKSFFSGVGFFMLRVQGPGTVFYGSYGALHEIEVGPEGYS
                     VDTGHITAFSGGLDYQVRPFGGFKGLFFSGEGLVCQFSGSGKVWVQTRDASGLAAFLH
                     PFRRVKSNSS"
     gene            19503..19877
                     /locus_tag="VFQ61_05095"
     CDS             19503..19877
                     /locus_tag="VFQ61_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953852.1"
                     /translation="MLATTETQTSPDARFRVEYQSHEIKLAHWIDQPRVIDMVTNTVL
                     LDLWPDVFSSWDGHVRFERAGVIRLELREFHARREKQDAAQESCVVEIDVNARRFSRS
                     GRPLPPPLVAALSVTLSEICSA"
     gene            19976..20452
                     /locus_tag="VFQ61_05100"
     CDS             19976..20452
                     /locus_tag="VFQ61_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9953853.1"
                     /translation="MTHPPSIPRVRASAVCVALGHILVVRLEDPVSRAVFSVVPGGLV
                     EPDESPAAAAEREVREETGLHVRVDPNRQRVERYPFTWSGQTFAVTTHFFAARLEPRL
                     SSEAGVNSSPTLPEPILDSGLPAYHLATYWLALDRAEAELGFHPAIWAATRALISG"
     gene            20445..21389
                     /gene="rarD"
                     /locus_tag="VFQ61_05105"
     CDS             20445..21389
                     /gene="rarD"
                     /locus_tag="VFQ61_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HET9953854.1"
                     /translation="MADSDLSRNGPDAAPSRSAGIGYALSAYALWGLLPLYIRAVHTV
                     APLELVSHRIVWAFSFLALVLVVTRRFEWLKTIQSRPRIIWSFLASAAVLSANWFVYI
                     WAADAGRVVDASLGYFINPLLSVILAVLVLKERLRPLQWTAIGVAALGVLWLTLLAGQ
                     LPWIGLSLAISFGCYGLLRKTAQLEALAGLTLETTLLLPAASAYLIWVAPRDGSAWST
                     GSPELRALLVAAGPITAVPLLCFAAGARRIPLSLLGLLQYLGPSLQLLLGVAVFHEAF
                     PFRKLVGFILIWLSLLLYTLEGWSVSRASAAAQRAQRT"
     gene            complement(21458..22429)
                     /locus_tag="VFQ61_05110"
     CDS             complement(21458..22429)
                     /locus_tag="VFQ61_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET9953855.1"
                     /translation="MATPKNEAKATDLIEDEPEQGGPRPFWSGTLTFGLVSIPVELFS
                     AYKSVRTSLRMLGPDHKPLSRRYFCPKEQKMIESDEIVRGYEIADGEFVLVTDEELES
                     LAPRKSRDIELKRFVDASAVPPLYFEQSYWLAPSGESVRAYHLLATTMDRGKKAGIAT
                     FVMRGKEHLVAILSDGGLLRAETLRFADELRTPQQVGLPAVEAPEPKAVKQARQAIHQ
                     LSSDRVDHSELKDSYAERLLKLVEEKKAAKAGLVKLKSSTSAEARAEGGDVIDLMEVL
                     KRSLARASNTGDAGAHSKRASSPAPKSLPSSKKPAKRRPKKASARAG"
     gene            complement(22527..23411)
                     /locus_tag="VFQ61_05115"
     CDS             complement(22527..23411)
                     /locus_tag="VFQ61_05115"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET9953856.1"
                     /translation="MAARAIWKASVCVGDVRVPVKLFSAIEDRTVHFRLLHERDHTPI
                     EQRMVHPETLEPVPYGDAQRGFMEGKDSLVVLEKNELAQLEPKESRDIEIVQFVKPEA
                     LPQQLYERPYYLGPDGPTKAYFALVNAIEAEGRIGVARWVMRKKEYAGALRVQGGYLS
                     LLTLRSVEEVIASSQLPKPKVGELNPKEVAMAEQLVAMFEDTFDPSSFRDEYRERVLA
                     FVEAKAAGKTVRMGKFKPRQLESNLSRVLEESLRKAKQLKAGTEKSSGATDSESPKAK
                     SVRPARHSDKSRAHEKVA"
     gene            complement(23891..>24619)
                     /locus_tag="VFQ61_05120"
     CDS             complement(23891..>24619)
                     /locus_tag="VFQ61_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953857.1"
                     /translation="GYKSGLQLEPAAAEVPSGGAVKGVVLKESTTALGSVTGDVNFVN
                     AGTQQTSVVLVVESTFNEALARGEVPIGLRQFPVTGHYSFTGVPLGDYVVLAAFENDA
                     LVRDPDTSIGGTAIQHVHVDSKEVSVAAFKLTGALAVMQPGAEQAEVMEGTPTFVWED
                     DSSEDGYEVVVHDTFGNEVWRNAEVPRVTGSATVSLPYGGPALERGYYQFRATSFRND
                     TARGNTTRVYISATEDLKGVFVIR"
BASE COUNT         4555 a         7703 c         7702 g         4659 t
ORIGIN      
        1 acgctgctgc agagcgaggg cgtgctcgtc agcgcgttcg cttctggtcg cgatgcgctc
       61 gaggccgtcg accaaattca acccagattg atagtctccg atatcggcct gccggactac
      121 gatggctatc aattcatgag cgatctgcgc agccgaccgg acgggcgcgg cgggaataca
      181 cccgctattg cgttgtccgc ggctcccgcc gaccgggatc gctcccgcgc gctgttggcc
      241 ggatatcagt ggcacatcac caagccggtg catcacgagg agctggtaga cgccattcgc
      301 tgcctgctcc gagttcggcc tatcgaaact agcgaaacga agcgagcaga gtgaccagca
      361 gctgagtgaa cccagcggct gagcgaaccc agcgattgac cgcgtggacg gagcagcgcg
      421 gccggcatcg aatttgctga atgcgcgggt atgaagagtt tcccccttcc cgttgccaag
      481 gttcgtagca acttttcaga cgctgtcgct gcagttcgta acggtcaacg cattcggttg
      541 acgagacacg gaaagccggt ttgctggatg attggcgagg cggaccacgc cttgttgctg
      601 cgcaaacgtc gggggaacgg ctcgaatggt tcgcgcgacg atcaagacgc gggctgaggt
      661 ggaggtgtgg cgggcgtaca aatttgcccc cgcgactttg ccacacgctc ttcctgctta
      721 gcccgccctc gacggggatt ggatagatgg ctggattctc tctgatatcg acatggatgc
      781 gctccggatt atgacctata acgtccacag ctgtcgcggt gtggatgggc gcactcgccc
      841 cgagcgcatc ctcaaggtca tccgcaagtc gaacccagat gtggtggcgc tccaggagct
      901 ggatggcccg gccgccagcc acgattttgc gcaagccctc ggcatgcgtc acttctacgt
      961 cgaggcgcgt agcaaaggca gtacgggctt tggcaacgct ctcctaacgc gcttccccgc
     1021 cgatctcgta cgccaggagg cgttgccaag gctcagccct cagcaggagc cacgcgcggc
     1081 tcaacggctg cgggtgcaca ccagctttgg cagcgtcgaa atagtgaaca cccatcttgg
     1141 tctatcggac gcagagcgtg tgctgcaagt agacaagttg ctcagtgtgg attggttagc
     1201 ggaagtgacc ggagcacagt attcggtgtt gtgcggggac ctgaacgcgc gcccgggctc
     1261 gcccgcatat cgccgtttga ccgagcgcct gcgcgacgcg cagacgagcc tgggtcaagg
     1321 ccaatccacg ttcccatcgc tgtttccgat cgtacgaatc gatcatgtct ttacgaacga
     1381 cctgctagtc gcgcgacacg cggaggtgcc ggtcggcacg ctagcccgtc tggcttccga
     1441 tcaccgcccg ctagtcgtgg atctttcgcc cgtgcaaacc aaccaggccc catttgcccg
     1501 cggcaaatcg agcagcagcg cccccttgga gcgcgacgta ttggtggagt agttgtgatg
     1561 cgaccgattt tgattcctgg agaaacgtgt tggcaagagt gcgaggtgga cgaagccggc
     1621 gccctgttcg acggctgtga ctactatcgt gccttctacc agcaggcgtt gcgcgcgcgt
     1681 ggccacattc tgattgccgg ttggcagttc aacacgaacg tacctctgtt gcgtggtgaa
     1741 gaggccaagt ccgcaccgtt accggtggag ttcatgccct tcttggccga gctcggccgg
     1801 cgtaacccca acctcggcat ctatatcctc gcttgggact acagcccggt gttcgccttg
     1861 gagcgcgagt ggttgcaaag ggaggccttc gccttcggca cccccgacaa cgtgcagttc
     1921 tgtttcgatg acaagcaccc tctcggcgcc agtcaccacc agaagttcgt cgtcatcgac
     1981 ggtgcgttgg cgttcgtggg cggaatggac ctagctcaag gccgctggga cgaccgcaga
     2041 cacctggcct cgaatccttt gcgttgggag gccggcgctc cgcagaaacc ttatcatgac
     2101 agcatggctt acgtgcgtgg gcccgccgcg cagcagctcg cccggctgtt cgccgagcgc
     2161 tgggaagcag ccacgggcga accgctcacc ctgccggtaa cctcgccccg agaaccgaac
     2221 cccatcactg gatcgattcc catcgaagcg aaacgagtcg cgattagccg gaccggtcat
     2281 tgcacgacca gcgacgcttc gggtgaaacg agctatcagg agattcagtc gttgttcttg
     2341 cgttcaatcg agcgcgcgga gcacctgatc tatctggaga ctcaatatct gacatcgcgc
     2401 gtgtttcacg agacgctgat gagacgcatg cgagatagcg cgcggccgtt ggacgtaatc
     2461 atcgtgatgc cggacgacgc tgatacgccc aaggagcggc tcgcgttggg ggcagcccaa
     2521 aaccgaattc tgcgttcgct gtgcgataca gccgcccggt ccggttcccg cttgcgcgtg
     2581 tactgcaccg tgcccgaagg cacgcttcgg gatgaaacgg ttccggaagg agcttggtcg
     2641 gaaggcccgc catcggagag aggcaggtcg gatggccctc ggtccgagag gcccgctccc
     2701 gagaccgcac ggcctgaagg atcagccccg gagccgcccg cgaccttcat tcactccaag
     2761 attctgttgg tcgatgaccg actcatttgc gtcggctcgg ccaacattac gaaccgaagc
     2821 tttacgctgg atagcgagct cagtttggct tgggaggccg agaatgatac cgacggagtt
     2881 gcgcgcagcg tggttaggat gcgcgccgag ttgctgtcgg agcacgccgg cgtagcctgg
     2941 gccccagagt tctttcgcct tcacggcttg atatcgcggc tcgatgcggc ggtggcgagc
     3001 gggaagacgc gcttgaagcg cagacgcatt ccgagcgccg ccagcgaaga gaccccgctg
     3061 ttcgacttga cgggtctctt cgatcccgag aagccgctcg atgagctagc gctggatcaa
     3121 atcctaccag gtagggaaac ggacgaggac ggtgccgcag gccctcgttc gggtccaatc
     3181 agcgaaatgc aggccgccag cgaaagaggc taatgtgtcg agcgtggcag acgtaaccgg
     3241 cgctcccgcg ctcgaccgcc gcaaaggcaa gcgcaagcaa tggttacgtt ggctgctctc
     3301 ggcagctttg ttgtgcgtcg caggggcgtt cgtatggcgt gaacttcgcg ggctctccgt
     3361 tcacgagatc tggctgcacg tgcgagagcg ttcgtgggcg cagatcggcg ccgcatgcgc
     3421 gctcgcggtc gtgaactacc tccagctcac ccgcttcgac gctctggcgc tgcgttacct
     3481 gaatcagcgt ctgccggagc gtcggttggt attggcatcc ttcattgcca cggcctttgg
     3541 acacaacatc ggtccttccg tcgcgagcgg aggctccatt cgcctgcgct tctactcggc
     3601 gtggggaatc ccggcttggc aagtcgcaac gctgatcgcg tttctgtcgg taacgttctt
     3661 catcggtttg tgtgcggtgg cgggcattac tttcgtttct tgctcggcgg cggacgcgga
     3721 gcacttgcct ttgccgctct ggttactgcg caccatcggc ggcgtgttct tggggttgct
     3781 cgtggcctat ctggcggtgt gcgcgcgggg gccaacgctg accttgagag gtaagcgagt
     3841 ctctcccccg cggatcggca ttgctctggg ccagatcgcg acgggcggct tggactggct
     3901 gatgatggcg gcgatcttct cgctgctgct ccccagccac accgcctcgt acttcgaggt
     3961 cttgcgctcg ttgctggtgg cgcaggttgc gggcgtattc agccaagtgc ccggcgggct
     4021 cggggtgttc gagtccctga tgctgagttc attgggccat atcgtgagcg cgcccgagtt
     4081 gctcggaacc ctgctgctgt atcgcgcgct gtacttcttt ctaccgctgc tcttcgcgct
     4141 gttgctgatg ctcgtgagtg aagtgcgacg gaaagcttga gactcacggc attcgctcca
     4201 ttggtccgct ctatcggtcg gaagttctgg gtacgaggca gcgatcctcc atcactttcc
     4261 aaaccgccgc cgcgctggcg cgtgcccccg cctcggagaa tacgagcccg tccgctccga
     4321 cgtactcggg gtgcggcgca aacagctctc gcaggtctag ccaatggcac gggaccgcgc
     4381 tccttccaca ggcgtttcgc agcaagggac ggagcagatc cagccctgcc ttgaggcccg
     4441 ggtcgccaac gggatcgcca tagaagaagt acacgatttg ctccacgccg ccttgcgcga
     4501 agcgctgaaa taacagctcc gcgccgctaa tcgcggcctt taccgcggga cactcgtcgt
     4561 tgagcatccc cgtgcatgga tcgttcagga agtcggtcgc gccgccatcc aggaccacca
     4621 acttcggagg ttccccctgg agcgcggtcg tgtactcgtt accgatactg aagggccctt
     4681 ccgctaaagt ggaagtcaga tgcgaggcat aattcctgaa ggtcatgccc tccgcgagct
     4741 ggcccgcact gaccgcgtgc tcggcgaggg cgctgccgaa gaccgtgagt tcgatcagaa
     4801 catcccccaa aatcacgatc tggtccggct cgtaactcgg cggagtacat tctgctaccg
     4861 gcgcgggggc ctggttttgt gggacgtcgc gacaataacc ggcgtcacac acgaagttct
     4921 gaccgagccg cgcgcagtcc gccgcaacca agcaacttgc gtcgcagact tggcctcggg
     4981 cttcgggttg gcaccggccc tcggcgatcc gcgcggccaa cgagacgcag cttgcgctgc
     5041 cggctagctc acgacatacc gcgtcgtcgc tgcagcgcgc ggtgcaaacc ccgcagatac
     5101 actcgagccc gtcgttgcaa ctcgccggac agactcgcag gaagtgcgtc tcgctcccgc
     5161 tgtcggcctc ggccccaccc cgacaagacc aagcaaacga caaggccgcc aaagcagcca
     5221 agacaatgcc gcgcttgcgc atctctttaa cggggcgtcg gttcatcttc actactcacc
     5281 tgaggctctt catcctcgtg cgtcgggggg acgggagcat tactgctgga tacattgctg
     5341 ctggtagcgg cgccgctggc tggagcgcga ctaggcgagg cgccttgctg aatcaagcac
     5401 tgaccggcgt acaggagcac ttgggtatag ctctccggca ccgggttgtg ggcgttgaac
     5461 ccttccactt tgtcgcgcag ctcgcctaac ttctggcgca gatcccgcag ggtacccaaa
     5521 gcctcctgct cgtggggatg cccgggccaa acgtcgaggg tgtacgtgct accacccacg
     5581 cggtcggcga gattcgggct gctgcgatga atgctgagcc ggcacgtgat ggtgttcacc
     5641 aaagccttga aatggtcgaa cacggccgcc tcccaaccca ccggtgaccc gagcgggagc
     5701 accagggcgc gagcgctgta gtagccctgc gtcgtgtcgc actcgacgcg tcccgcctct
     5761 accaagcgtg ctagcgactc tgtcagttcg cgctcggaaa tcggcttttt tgccaggtta
     5821 gcgaacgaac acggcccctc ccggtagatc atcgcccaca agagctcgtc gaccccctcg
     5881 ctagagttcg attgccgcag ctgctccagc tcctcttccg gggtcaggcg gtacttcatg
     5941 cgtgcgcccg tgccggacac gctcactagc ccgctctcgc acaagtcgtg cagcacgccc
     6001 ctcacctggc tctcgtcgtc acccgcaaac ttctcgagca cctgagcgcg ggtgaccgtc
     6061 tcgctgcttt gaacgaagtc gagcacggcc acccacagcg atcggccccg atccgtagag
     6121 ctctccgtgt agcgctgaat cttgcgttga tacgtgcgta gacccaggcc gaacatatcc
     6181 gcgctgacct tgcggctcac gccttgccgc tcgagctcat gcaccaggtc caagaacact
     6241 tgattggcga cctgggccag gggcgcccgc gttcccccgc tggtcgcgag ctgcgcaatc
     6301 aatatcgtgg tttgacgcac gatagcttcg atcaacaact cgatagtcat atccgcggcg
     6361 ccatgctcag ccgttcgcgg tgccgtctgg cggcccgccc gacttaaccg taatgctgag
     6421 cagccgggag cgacaagttt gtcatccccc ccagcccgtg ttcgcgcgtg gcgctgaggc
     6481 tgcacgagca tggctcggag tgaaccagag cggccgctgc ccggtccgcg atgcgcacct
     6541 cctatggcag ctcttagctc gcctccgctg ccgcgagacg attcgcggcc agccggagtt
     6601 gtgcgtcgag gtcggcgtga tcgacgggct tgatcaggta cgcgtcgaag ccggccttgg
     6661 ccgcgtcatt gcgcacctcg gcgtgcccat agccggtcac ggcgacgagc cgcgtgcggc
     6721 tacccaggat cctgcgcaga gcctcaccca cctctgctcc tcccataccc ggcaagccga
     6781 tgtcgatcag ggccacgtcg aaatgctggg agctcgcgag ctcgatggcc gcgtgcccct
     6841 cttccgccat ttgcacgtgg tgacccccgc gacgcaacat ggccgaggtc aactcgcgaa
     6901 tgtcttcgtt gtcctcgacc agcaagacct tgaggcggcg ctgggttccc gaaacccgta
     6961 ccccgggagc ctcgttgtcc gcgcgctgct gagcgtccgc gagctcctga gacaacagcg
     7021 gcaaacgcac gcgaaaagtg ctgccgcggt ccacgccctc actgaaggcc tcgaccttgc
     7081 cgccgtgcag tacgaccaaa tccttgacca atgagagccc gattccgagg ccgctcgtct
     7141 cttcgctgct ctcaccttgc tcgaagatgt cgaacgcgcg ctccaccagg tcgggagtca
     7201 tgccccggcc gttgtcgatc acctcgagca ccgcatagcc ctcttcctta cgacacttga
     7261 gctgaatctg cccttgcggg ggggtgtacc gcgcggcatt ggtgagcaga ttcgtgacca
     7321 cttgggtgag cctcacccga tcgccatcga cgtaaaccgg gctcgaacac tcctctactt
     7381 ggagacgatg tccgcgctcc tcgatcaatg ggcgacacaa ctccaccgct cgctcgatcg
     7441 catcacccaa agaaagcggc gctcgacgaa gctggatctt tccctgagat atgcgtgaga
     7501 cttccagcag gtcatcgacc aaacgaacca gatggcgaat ctgccggcgc atcgccgtga
     7561 gcacgttgct gacctcgctc gcgtcgctca atagctccgc gagcagatcg acgcccaaag
     7621 cgatgggtga gagcggtgtc cgcagctcgt gcgcgagagt agccaaaaac tcgttcttgc
     7681 gtcgatcggc gagctccagc tcacgcacgg cgcgcgtgag ttctgcctgc gctcgctcgc
     7741 gctcggcaac ggtagcggcc agctgctcgg cgcgcttctt gttctcttca gcaactcgac
     7801 gcagcgtgtc cgcctcccag cgccgacgct cctcgttcag ccggtcctct tgttcacggc
     7861 gctcgtgttc acgcagcaac ttggcctgac gcgcgatttc cgcgacgtgc ttgcgtagct
     7921 ctacgaacgc cgccaccttc gctttcagga tttctggact gatcggcttg aagaggaaat
     7981 ccaccgcccc gagctgataa ccgcgcacca cttcatcgtg cttttgatcg taagccgtga
     8041 cgaagataat cggcgtgtgc ttgcagcgat cccggctgcg aattagacgc gcggtctcga
     8101 agccatccag acccggcatc tgaacgtcta gcaaaatcac ggcgtagtcg tgcttgagca
     8161 aggcccgcag cgcgtcctcg cccgatttcg ctttgacgat gcgaccggcg aaatcaccta
     8221 gcgcggcgtc gatggcgagc agattgcgtg ggttatcatc gaccaccaag atgtcggtga
     8281 ctgactcttc ggcagcctcc acggcgttca cggcgctcca gtcgttcatg tgcgctcact
     8341 gtgcaagcaa agcgggctct gcatggcgag agctccttgc atggcgacag ctacctgcat
     8401 atccggcgat ccctgcgtgc aacgaagaac gtgcaagcag agaaggccct gcggagcgtg
     8461 cagcgacggc tctcgagaaa tcatacgtcg catactacta tgctcccgta gcccaagctc
     8521 aaagcgtatg atggccaggt tccgcgaaaa catcgcctgc cccgcgcgag agaaagaaaa
     8581 cctttgccga tgcaaaacgc cacaacgaac ggccgcaaga ccgcctcaac gggcaagact
     8641 gactcattgg aacgaaaagc cattttggcg ggcctgcgcg cgcttcggcg cggagagttc
     8701 ggcacccgtt tacctgatgg gttgattggc atcgacgggc aaatcgccga ggcattcaat
     8761 gatatcgcgc tacagctcgc gtctctggaa ggagaactcc aggaagttcg ggtcgcggtc
     8821 ggcaaagaag gccgcaataa acggcgtctt cagcgcagca tcggcaagaa cggctgggag
     8881 agtatcgtcg gctcggtcaa tgagctcgta gatgacgtca ctacgcatac cgcggagctg
     8941 accaacgtgg tcgaggccgt aacgcgagga gacttctcgg ttcgcgtgga catcgagggg
     9001 cgcgcggaac cgttgcgggg cacggcgtta cgccaggccg agtccgtcaa taagatggtt
     9061 ttgtacctgg attcttatac ccgtgatctg atccggctcg cgatcgaggt cggggtggac
     9121 gggaagctcg gcgcagagtc acgccagcgc gaagtgaccg gagtttggct agagctcgga
     9181 aacgcggtca acctgatggc cgcgaacctc accgcccagc tgcgtgaaat cgcgcgcgtg
     9241 accaccgccg tggcccaggg tgatctcagc aagaccatcg agatcgaggt acgcggtgaa
     9301 attctcgatc tcaaacacac catcaacacg atggtcggcc agctccgctc cttcgcggac
     9361 gaggtgacgc gcgtagctcg tgaggtgggt accgagggca tgttgggcgg gcaggcccgt
     9421 gtacgcggcg tctccggcgt ttggcgcgat ctgacggaga gcgtcaattt catggccgat
     9481 aacctcacca cccaggtgcg taatatcgcg gaggtgtcga ctgcgatcgc ccgcggcgac
     9541 ctgagccgga agattaccgt ggccgccaaa ggggagatcc gtgacctggc cgccaccatc
     9601 aacacgatgg tcgaccagtt gagcgcgttt gcctccgagg tcacgcgcgt ggcgcgcgag
     9661 gtgggtacgg agggcgtact gggcggccag gcgcaggtca cgggagcctc aggagtttgg
     9721 cgcgagctca cgcaaaacgt gaactcgatg gcgaacaacc tcacctctca ggtgcgcaac
     9781 atcgccgagg tggtgaacgc aatcgccgag ggcgatctcg gtcgcaagat cacggtcgat
     9841 gcgcggggcg agatcttggc cctgaagaac acgatcaacg cgacggtcga caagctgaac
     9901 cgcttcgccg ccgaggtgac gcgcgtggcc cggctcgtgg gcaccgaggg cacgctcggc
     9961 gtgcaagccg aagtgcgcga cgtatccgga atttggaagg atctgacgga cagcgtgaac
    10021 cacatgggtc gcaacctgac cgatcaggtg cgcgacatcg cggccgtgac tactgccgtc
    10081 gcgaacggcg acctctcacg caagatcacc gtggaggtca agggcgaaat cctagccctg
    10141 aaaaacacca tcaacgacat ggtggatacg ctctcttcct tcgcggacga agtgacccgt
    10201 atggctcgcg aagtgggcac cgaaggcgta ctcggcgggc aggccgaggt gcccgatgtg
    10261 tcggggattt ggaaagagtt gacggacaac gtgaactcga tggccaacaa cctgaccaac
    10321 caggtgcgta acatctccga ggtggccacc gcggtggcct ccggcgatct ctcccgcaag
    10381 atcacggtgg acgcgcgcgg tgaggttcaa gacctcaagg aaacgctcaa cgcgatggtc
    10441 gagcagctca actcgttcgc ggcccaggtg aaccgggtcg cgcgagaggt gggcgtggac
    10501 ggacgactcg gcggccaagc cgtggtcgag ggcgtgcgcg gcacgtggaa ggagctcacg
    10561 gacaacgtga acctcatggc gagtaacctg accaatcagg tgcgcgatat cgctgaggtg
    10621 accacggccg tggcgcgcgg tgacctgacc cgcaagatcg ccgtcgacgc gcgcggcgaa
    10681 attctcgagc tcaagaacac catcaacacc atggtcgacc agctcggctc cttcgctgat
    10741 caagtgatcc ggttggcgcg cgaagtgggt gtggaaggca agctaggcgg tcaggcagac
    10801 gtgcgcggtg tctcgggcac ttggcgtcag ctgacggacg ccgtgaactc gatggccaac
    10861 aacctgaccg atcaggtgcg caacatctcg caggtcgcga ccgcgatcgc ccgcggcgac
    10921 ctgaatcgaa agatcgtcgt ggatgcgcgc ggcgagattc tcgagctgaa gaacaccatc
    10981 aacacgatgg tggaccagct gagctcgttc gccgaagagg tgacgcgcgt ggcccgtgaa
    11041 gtgggcaccg aaggtcggct cggtgggcaa gcctccgtgg aaggcgtgcg cgggacctgg
    11101 aaggaactga ccgacaacgt gaatcagatg gcgagcaacc tgaccaatca ggtgcgcgac
    11161 atcgcccagg tcgcgaccgc gatcgcccgc ggcgagctga gccggaaggt gagcgtggag
    11221 gtgcgcggcg aaatcctgga gctgaagaac atcatgaaca ccatggtgga tcagctgagt
    11281 tcgttcgccg acgaagtcac gcgcgtcgcg cgcgaggtgg gtgtcgaggg caagctcggc
    11341 ggccaagcgc gcgtgcgcgg cgcctcgggt atttggaaag agctgaccga caacgtgaac
    11401 ctgatgggct cgaacttgac cgatcaggtg cgcgacatcg cggaggtgac cacggccgtg
    11461 gccaccggcg acctgtcgcg caagatcacg gtggaggtgc gcggcgaaat cttggagctc
    11521 aagaacacca tcaacgtcat ggtggatcag ctccgctcgt tcgccgacga agtgacgcgc
    11581 gtggcgcgcg aagtgggcac ggaaggcgtg ctcggtgggc aagccgaggt gcgaggcgtc
    11641 tcgggtacct ggcgcgagct gacggacaac gtgaacttca tggcccgtaa cctcaccgct
    11701 caagtgcgcg gcatcgcgcg cgtggtgacg gcggtggctc gcggcgacct ggatcaagag
    11761 ctgcggctgg aagcgcgcgg cgagatcgca gagctggtgg aaacgatcaa cggcatgacc
    11821 agcacgctct ccgtcttcgc cgagcaggtg accggcgtgg cccgcgacgt gggcgtggaa
    11881 ggccgcctcg gtggccaggc cgacgtgcct ggcgcggccg gcgtgtggag cgatctggtg
    11941 agcaacgtga acgagctcgc cggcaacctg acccgacagg tgcgcgccat cggggacgtg
    12001 gccacgtccg tgaccaaggg tgatctcagc cggtcgatta cggtcgaagc ccgcggcgaa
    12061 gtcgcgctgc tcaaagacaa catcaaccag atgattcaca cgctggcgga gacgactcgc
    12121 gtgaaccagg agcaggactg gctgaagacc aacctgacgc gcttcacccg tatgctgcag
    12181 ggccaacgcg acttgttgac cgtggcccaa caggtgcttt cggagctcgc atccgtagtc
    12241 ggcgcgcagc atggcgcctt ctacatggcg agcaaagaca gcggcgagac gacgctgcag
    12301 ttgttcgctt cctacgcctt cgtggagcgc aagagtgcgt ccaatcggtt ccgactgggc
    12361 gagggtctgg tcggtcaatc cgcgctcgaa aagaagcgaa tcttggtgac ccccgcgcca
    12421 acggattacg ttcagatcac ctcgggtctc ggcagcgcac cgcccatcag catcgtcgtc
    12481 gtcccggtct tgttcgagtc cgacgtgaag ggagtgatcg agctcgccac gttcgaacgc
    12541 ttcacgcccg ttcaagtcgc gttcctggag cagctgctgg aaagcttggg catcgtggtc
    12601 gcgaccatag aagcgaccat gcgcacggac accctgctcc gtcagtcgca gcggctcacc
    12661 gaggagcttc agtcccagca agaggagctg caacagacca acgaggagct cgaagaaaag
    12721 gcgcaccagc tcagcgagca aaaggcggag gtcgaacgca agaaccgaga agtggagacc
    12781 gccaagcgcg agctcgagga gaaggccgaa cagctggcgc tcacgtcccg ctacaagtcc
    12841 gagttcttgg ccaacatgtc gcacgagctg cgcacgccat tgaacagctt gctaatcttg
    12901 tcgcgccaac tcggggacaa cgcggaccgg aatctgaccg agaagcaggt tcgctacgcg
    12961 caaaccatcc accagtcggg cgccgatttg ctggccttga tcaacgagat cttggacctc
    13021 gccaagatcg agtccggcac tatggcggta gatgcctcgc cggtccggct ctcggagctc
    13081 accgactacg tcgagcgcac tttccggcac ctcgccaacg agaagaacct cgagctgttc
    13141 acggcgctca ccgaggaggt ccccgaaacg gtcgagaccg acgacatgcg actgcgccaa
    13201 atcctgcgca atttgctatc caatgccatc aaattcaccg agcgcggcga agtatcgctg
    13261 cgcgtgtgcc cggcaaaggc gctccccaat cggccctcgt ccgtcaatcc cgacagcatc
    13321 gccttcatcg tcaaggacac gggcatcggc atcccccgcg acaagcaacg catcatcttc
    13381 gaggcgttcc agcaagcgga cggcagtact tcacgcaaat atggtggtac cggccttggt
    13441 ttgtcgatca gccgcgagtt gaccgcgctg ctcggcggcg aactcacggt cgagagcgaa
    13501 ccaggagcgg gcagcacatt caccttgtac ttgcctcggc gagttccgga ctcgagccgg
    13561 cgcaacgccg cgcgcagtga accgcttccc cccgttcaga ccaagccgac gccctcgctg
    13621 cctcgccgcg aggggccgcg catcgatgat gaccgcacca acatccgccc gggtgatcgc
    13681 gtgctcttga tcatcgagga tgatcagccg atcgcggagt tgctgctcaa catggcgcgc
    13741 gagcgcggtt tccgtggtgt cgtgacgatg catggtcagg aggccctcga ggtcgcacga
    13801 tcgctgcgac cggatgcaat cacgctcgat ctccggctcc cggacatcga cggttgggtg
    13861 ttgctggatc agctgaaaca cggccccgaa acgcgccaca tcccggtgca cgtgatcagc
    13921 gcgttggacg gtgagcggcg tgcgctcgaa aacggcgcat tcgccgtgct gcgcaagccc
    13981 gccacccaag aagcgctgat gtccgcgctc aattcgctgg cgggcttcct ggagcgccgc
    14041 gtacgtcatc tgctcgtcgt ggaggacgat ccggtccaac ggcagagcat cgtggatctg
    14101 atcggcgacg gcgacgtcgt cacgaccgcg gtgggcagcg gcgaagaggc gctcgcgctg
    14161 gccaacgaga agaacttcga ctgcatcgtc gtcgacttgc gtctgcccgg gctctcgggc
    14221 ttcgacttga ttcaccagct caagcaatcg ccgaacatga gccgcacccc catcgtggtg
    14281 tacaccggcc gcgaattgac ccgcagcgaa gacgcagagc tgaatcgtat ggcccagtcc
    14341 gtgatcatca aggacgtgcg ttccccggag cgtctactgg aagaaacggc gctgttcctg
    14401 caccgcgtgg aggccaacct gcccgaagac aagcgcgatc ttttgcgccg agtcgcgcag
    14461 atggatccgc tgctgtccgg taaacgcgtg ctgctcgtcg acgacgatct gcgcaacatc
    14521 ttcgcgctga ccgccttgct cgaacagcac aagatgttgg tgcgcagcgc ggagaacggc
    14581 cgtgaagcgc tcgaacttct cgacaaggat tcggtcgata tcgtgttgat ggacatcatg
    14641 atgcccgaaa tggatggtta cgaagcgatt cggcacatcc gcaagaaccc caaacacgcg
    14701 cagctgccgg tgattgccct gacggcaaag gccatggccg gcgatcgcga cgcgtgcatc
    14761 gaggccggcg cgtccgatta catcaccaag ccggtcgatc tctcgcagct gctctcgctg
    14821 ctgcgtgttt ggctgtacgg gcggagccgg gcatgagtct cagcaccgcg ggtgtggagc
    14881 aggcggagat cgaagctttg gtcactgcca tcctgcagcg ctacggctac gatttctccc
    14941 actacgcgcg cgcatcgttc gtgaggcgag tgcggcacgc gctgaacgag gaggccttgc
    15001 cgagcgtgtc ggcgctgcag gccaaattgc tccgggatcc gctcgcgatg cgccgtttct
    15061 tgacctacct ttcggtcaac gtaacggcaa tgttccgtga tgccgagttc tacgaggtgc
    15121 tgcgcaaaca ggtgatcccc tggttgcgca cgtacccctt cgtacgcatt tggcatgccg
    15181 gatgcgctag cggcgaagaa gtctattcga tgtgcatcct gctcgaggag gaagggattt
    15241 acgaccgttg caggatctat gcgacggacc tgagcgaggt ggtactacac cgtgcgcgca
    15301 tggcgactta ccctttgtcg ctcgtgcagc gttacacggc taactaccac aaggccggcg
    15361 gtgaactcga gttctccagg tattacacgg ccgaccagga gaacgcgatt atccacgagc
    15421 gactgaagaa gaacgtcgtc ttctcgttac ataatttggc gtctgacggc tcgttcaacg
    15481 aatttcacct ggtttgctgc cgcaacgtca tgatctactt cgacgaggtt ttgcagggcc
    15541 gcgtgctaac cttgttcgat cagagcctct ccagaggcgg ttacttggcc ctgggccggc
    15601 gcgagacctt gcgattcagt agcttggcgc agacctatac tgagattgag ggcaacgtta
    15661 agatatatcg caagccgcga taagagcgag tcgtgatgga agcgatacgc gccatcgtcg
    15721 gaatcggggc atcactcggc ggcctgacgg cgtgccgcca agtgctctgg gacttgccgt
    15781 ccaactttcc agctcccatt ctaatcgtgc aacatcggtt ggcggaggcg gagccgttgc
    15841 tcgccggctt gctcggcaaa gaagacgggc tacgcgtcgt cgagccggac gacaaggccc
    15901 tgctgagtcc gggcaccgtc tatgtggcgc cgagcaatta ccatatgttg gtggagcaag
    15961 gcggaaccag catcgccctc tcgaccgaag gcgtggtgcg ctacgctcgc ccctcgatcg
    16021 acgtattgtt cgagtcgatg gctgcaacgt tcgggcgcgg tgcggtcgcg gtcgtgttga
    16081 ccggatctag cgacgacggg gcgcgaggag cgcaggccgt caaacgcgca ggggggcggg
    16141 tcatcgtgca aacgcccacc caagccgaga gtcgaatcgc acccgccgcc gcgatcgccc
    16201 gaatgaagcc ggacgccgtc gtggatgtcg aggcaatcgc gcctttgctc cgcagttggt
    16261 tgagagcctg acctgtacgg ctcagggtcc acagcggatt ggcttgggca gtatcggcag
    16321 tgcgcgagct ggcgttggat tgccagacgt gctcctcgaa cgcgcccgcc tggcgcgctc
    16381 accgggatcc ggatgagtcg agaagaattc gggtggcgag cgaccctgtt cgaccattcg
    16441 atcgaagaac tgggccaacc ccgacgcttc gattccacaa cgctctagga gctcgattcc
    16501 cgctcggtct gcggcaagct cttcgtctcg ctcgtaacgc aggctcgcca ccttggctcc
    16561 gatggcagcg tgagacttga gatccgagcc gcccagcagc aaggacagcg cgaagcttcc
    16621 acccatctcg cgcagtaggc ggcgggtgcc gtgtcggagc accacgtgct gaagttcgtg
    16681 ggccaacact ccggccactt cttcactgct ctgagccgcc tgaagcaacc cggagttgac
    16741 cgttacccac ccgcccggca acgcgaacgc attgacggtg gcatcatccg cgacgcgaaa
    16801 ctggaacgag aacggcgtgt gtaccccgca ctcggtcagc acccgggcaa gctcctggac
    16861 gtatgccttg gcgccagcgt caggacaatc ggcctgcccc agagaggcct cggagagttc
    16921 accgagtctg cggtcgacgc tcaacggcac ccacggcgtg agcgactccg ccaacacgcc
    16981 gccgccccag atcgccgaac cgcccaacgc cagcaagact gcgccaagca ccgcgagctc
    17041 gatccaaact ctcgaagagc tcggcttgaa accgcgtgtg gcaaccggcg ttccctccgg
    17101 cgggggcggt gcaggggcgg gtgctggcgc cgccgcgctt ccggataagg gcgccgcgct
    17161 tccgggagat cccccctcat ttgcatgcgg cgctaccccg cttgcgcctg tgagctcagg
    17221 tgacgtagtg cccggggcgg gctgtttcgg ttcgttatct tcgtcttcca agaccttcac
    17281 gggggggccc cagcagcgtg cggctccgag caggcagctt gcaaccagga accaaccgtc
    17341 gcaacgtccc atccctggtg gacgcatgaa tcactacgtt gagcacggtc cttcgttcgc
    17401 gtggctcaaa atccaactcg aggccggtga gacgctacag gcagaagccg gcgccatggt
    17461 cgcaagggag cctcacgtgg agatggagac gcgactgaat gccggctcga gtccgggcgt
    17521 tttcaggcta atttgggccg tcatcgtggc cttcgtccgc aagtttctgg gcggagagac
    17581 gatgttcgtg aatcgcttct cggcaccagc gcgaggagaa gtcatccttg cgcccgccct
    17641 gagcggccat gtcatcgccc agcgcatgcg gcccggcgag aggctgttcg tgcaacgggg
    17701 ctcgtacctg gcttcgtccg gctcggtgga cacccgcgtt cggtttacgg gcctgcgctc
    17761 gctgctcggc ggtgaaggtc tggtgctgct cgagtgctcc ggagaaggtg acctctggct
    17821 caactcgtat ggtggggtat tgccgttgcc ggtcaacgga acgttcacag tagatacggg
    17881 gcacatcgtg gccttcgacg gcacactgga ctttcgcgtc aagagcgtgg gcggcatgaa
    17941 gtctctgctg ctatccggag aaggcctggt ctgtgaattc tccggacgcg gcacggtcta
    18001 tattcaatcc cggaacttgc aggctctagt cggctggctc agcccgctgc tgccctcctg
    18061 aagggaaaca tgcacatcga cattcgatat aatccagctc attccctggc catggtccac
    18121 ttgcagccgg gagaatccgt tcgggcggag gcttcggcga tggtgagcat gacgagcaac
    18181 atcgatgttc gcacgaccgc cacgcacgcc tccggaaagg gcctgctcgg cggggtgaag
    18241 cgcgcgttgg tcggcggaga atcgttcttc cagaatctct acaccgcgcg ctccagcggg
    18301 gctcacgtga accttgcgcc acgtttatgc ggggccatgg tggttcacga attggaaccc
    18361 cacgccgatc tgttcattca aggctcgagc tacgtcgctt cggccgagtc ggtccagata
    18421 gatacccaat ttcagggatt tcgcggcctc ttcagcggag agtcgttctt ctttctgaaa
    18481 gtatcggggc gagggccagt actcatgaac gccttcggag cgatcgaagc gcgtgagctc
    18541 gacggggagt tgatcctgga cacgggccat ttggtcgcgt tcaccgcggg tatcgagtac
    18601 tcgataagca aggccggagc gggctggatc agcagttatt tgagtggaga agggctcgtg
    18661 ttacgcctac gtggaagagg cagattgtgg atccagagtc gaaatccgcg tgagttcggc
    18721 accctcgtgg gccgcctttt gcccgcccgt gaaagctaac gccatgaagc atcaaatctt
    18781 ggactcaccc gactttgcca tgctggagtt ggtactcgat gcgggggact ccgtcgtcgc
    18841 cgaaagcggc gcgatggttg ccaaagactc gcaaatcgag ctttcaacca atatgcgcgg
    18901 gagcttcctg catgccgcca agcggaagct gctcggaggt gagtcgctct ttcaaaacac
    18961 attcagagcg actgccaacg ggcagcggct ctatctcgct gcggcggccg aaggcgattt
    19021 gaaggcgcat gtgttggcgg caggggaagc tttgttcttg cagagcggcg cgtacgtggc
    19081 ccatgtcggc tcggacctcg tattggatac tcatttcgcc ggactcaaat ctttcttcag
    19141 cggcgtcggt ttcttcatgc tccgcgtaca ggggccaggc acggtctttt acgggagcta
    19201 cggagcgctc catgaaatcg aggttgggcc tgaaggatac tcggtagaca ccgggcatat
    19261 tacggccttc agcggcggcc tcgactacca agttcgacct ttcgggggct tcaaaggtct
    19321 gttttttagc ggtgaagggc tagtctgcca gttcagcggg agcggtaagg tttgggtgca
    19381 gacgcgagat gcgtccgggc tggccgcctt tcttcacccg ttccgtcgtg tgaagtcgaa
    19441 cagctcttag cgcgagtttg ctccgcggca gagcttcgca atcagcagca gagagggaac
    19501 tgatgctcgc cacaaccgaa acccaaacgt ctccagacgc gcgattccgc gtggagtacc
    19561 agtcacacga aatcaaattg gcgcactgga tcgatcagcc ccgagtcatc gatatggtga
    19621 cgaacaccgt gctactcgat ctctggccgg atgtcttttc gagctgggac gggcacgttc
    19681 gcttcgaaag ggccggggtg atccgcttgg agcttcgcga attccatgca agaagagaga
    19741 agcaggatgc ggcccaagag agctgtgtcg tggagattga cgtcaacgct cgccggttct
    19801 cgcgctcggg tcggccgctc ccccctccgc tggtcgccgc attgagcgtc acgctgagcg
    19861 aaatctgctc ggcgtaagtc tccgctcggc tggttctcca aaacctgctt tgcgtcttgg
    19921 cgggtggcgt gccagagggg acatgccgcg gagcggctct gccgctagac ctcccatgac
    19981 ccacccgcct tcaattcctc gagtccgagc gtccgccgtg tgtgtcgcgc tcgggcacat
    20041 tctggtcgtg cgcctcgagg atcccgtctc gcgcgcggtg ttttcggtcg taccgggtgg
    20101 gctggtggaa cccgacgaga gccccgcggc cgccgccgaa cgcgaggtcc gcgaagaaac
    20161 aggcttgcat gtacgggtgg accccaaccg gcagcgcgtc gagcggtacc ccttcacgtg
    20221 gtcagggcag accttcgcgg tcactacgca ctttttcgct gctcgcttgg agcctaggct
    20281 ctcatcagaa gcgggagtga actcgagccc gactttgccg gagcccatcc tcgatagcgg
    20341 cctcccggcg taccacctcg cgacgtattg gctcgcgctg gatcgggccg aagcggaact
    20401 tggctttcac ccggcaattt gggccgcaac acgcgcgttg ataagtggct gattctgacc
    20461 tctcgaggaa cggtccggat gctgccccct cgcgaagcgc tgggattggc tacgcgctgt
    20521 cggcatatgc gctgtggggt ctccttccac tctacattcg cgcggtgcac acggtggccc
    20581 cgctcgagct ggtgtctcac cgcatcgtgt gggcgttctc gttccttgcg ctggtgctgg
    20641 tggtcacgcg gcgcttcgaa tggctgaaga cgatccagtc tcggccgcga atcatctgga
    20701 gtttcttggc cagcgcggcg gtgctgtccg ccaactggtt cgtctatatt tgggcggccg
    20761 acgcgggtcg cgtcgtcgac gcgagcttgg ggtacttcat caacccgctg ctcagcgtga
    20821 tcctcgcggt gttggtgctc aaggagcggc tgcgcccgct ccagtggacc gccattggag
    20881 tggccgcgct cggggtgctc tggctaacgc tgctcgctgg ccagctaccg tggatcggtc
    20941 tgtcgctggc aatctcgttt ggctgttatg gcctcctgcg caagacggcg cagctcgaag
    21001 cgctagccgg gctgaccctc gagacgacac tgctcctgcc cgcggcaagc gcgtacttga
    21061 tttgggtcgc cccgcgggat ggaagcgcgt ggagcacggg ctctccggag ctccgcgcgt
    21121 tactcgtggc ggcgggcccg atcacggcgg tgccgctgct gtgctttgca gcgggagcgc
    21181 gtcgcatccc gctctccttg ctgggtttac ttcagtactt gggaccctcc ttacagctgt
    21241 tgctcggggt cgctgtgttc catgaagcgt tcccgtttcg taaattggta ggcttcatcc
    21301 tgatttggtt gtctctctta ctctacacac tcgagggttg gtcggtctcg agagccagcg
    21361 cagctgctca gcgggcccag cgcacgtaag atagccaatc aatctctggt gcgacaaggt
    21421 cgatggctca cgtcagtgac ggcgctagtc tgcggcgcta gccagctcgt gcggacgcct
    21481 tcttgggacg acgcttagcg ggcttcttgg aagagggcaa cgacttcggg gctggtgatg
    21541 atgcgcgctt cgagtgcgcc ccggcgtcac cggtgttgct ggcgcgagct agactccgct
    21601 tgagcacctc catcaagtcg atcacgtcgc ccccctccgc gcgtgcctca gcggacgtgg
    21661 agctctttag cttgaccaaa ccagccttgg ccgccttctt ctcttcgacc agtttcagca
    21721 gccgttcggc gtaactgtct ttcaactccg aatgatcgac tcggtcggag ctgagctgat
    21781 ggatggcctg ccgtgcctgc ttcacggcct tgggctcggg cgcttcgacc gctggcaagc
    21841 caacctgttg gggcgtccgc agctcgtctg cgaagcggag agtctccgct cgcagcaagc
    21901 ccccgtcgga gaggatcgca acgaggtgtt ccttgcctcg catgacgaac gtcgcgattc
    21961 cggccttctt tccgcggtcc atcgtggtcg cgagcaggtg atatgcgcgc accgattcgc
    22021 cgctcggggc gagccaataa gattgctcga agtagagcgg aggcaccgcg gaggcgtcca
    22081 cgaaccgctt gagctcgatg tcgcgagact tgcggggagc cagggactcg agttcttcat
    22141 ccgtaacgag cacgaactcc ccgtctgcaa tctcgtagcc gcgcacgatt tcatcactct
    22201 cgatcatctt ctgttctttc ggacagaagt agcgacggct cagcggcttg tgatcggggc
    22261 caagcatgcg cagtgaggta cgcacgctct tgtatgcgga gaacagctcg acgggaatgc
    22321 tcacgagccc gaaggtcagg gtccccgacc agaaaggccg aggcccccct tgttcaggct
    22381 catcttcgat tagatccgtt gctttggcct cgtttttagg tgtagccacg acgctgtact
    22441 cctgtaagag cgtagtcctc ggaaaccgaa tcccgccaaa acttactcct cgagaaccgc
    22501 cacgccgcga gcatgatccg acagcctcaa gcaaccttct cgtgagctct ggatttgtcc
    22561 gagtgtctcg cgggacgcac actcttggcc ttcggagatt cggaatccgt ggctccagaa
    22621 gacttctcgg ttccggcttt gagctgcttc gctttcctga ggctctcctc gaggacccgg
    22681 ctcaagttgc tctccagctg gcggggcttg aacttgccca tacggacggt cttacccgcg
    22741 gctttggcct cgacgaaagc cagcacacgc tcacgatact cgtcacgaaa tgagctcgga
    22801 tcgaaggtat cctcgaacat ggccacgagc tgctcggcca tcgctacctc cttcgggttg
    22861 agttcaccca ctttaggttt cggcagctga ctcgatgcga tcacttcctc caccgatctc
    22921 agggtgagca aggagaggta cccaccttga acccgcagcg cgcccgcata ctctttcttt
    22981 cgcattaccc agcgcgcaac cccgattcgg ccctcggctt caatcgcgtt cacgagcgcg
    23041 aagtatgcct tggtcggccc gtccggcccg agatagtagg ggcgttcata cagctgttgg
    23101 ggcagagcct ccggcttcac gaactggacg atctcgatgt cgcgggactc tttcggctcg
    23161 agctgtgcga gttcgttctt ctccaacacc accagtgaat ccttgccctc catgaacccg
    23221 cgctgggcat caccgtacgg gacgggctcc agcgtctcgg gatgcaccat gcgctgctcg
    23281 atcggagtgt gatcgcgctc atggagcaag cgaaagtgca cggtgcgatc ttcgatcgca
    23341 gaaaacagct tcacgggtac gcgaacgtcc ccgacgcaaa cggacgcctt ccaaatcgcg
    23401 cgcgcggcca tgattcagcc tctccctcgc gttctcggtt tacgtttgcg ccgcatggag
    23461 tccgtagctt cggcatcttg gagaggatcg accgtcgtgc actcctcgca cgagaactcg
    23521 cgcgtctccc gaatgcgcac gatgcaatgc gggcagagcg gcgcgtcaca gctggtgcag
    23581 cgcacctcta ggatgcgcgc gtagccttga agacaacgtg aacagacgac gctagcgtcg
    23641 gccaaccacc acggcgaatc ggctgcagcc atcgaattgg tgtgcggaac tgcaatcacc
    23701 gttccacacc ccgttcgacg cttggagcct acgtttgcgg caaagccgca ctttacgccg
    23761 tgcacacgaa cgccgcatga ggcacatcga aacggctcga actcgcggag ggctccgcgc
    23821 gtccgggcga ggctcggggg acctcgcccg gcgttctcgg ttttcgagcg ccagcgtcaa
    23881 cggggcgtct tcagcggatg acgaaaaccc ccttcaggtc ttcggtggcc gagatgtaaa
    23941 cgcgcgtggt gttaccgcga gccgtgtcgt tgcggaatga ggtggctctg aattggtaat
    24001 agccgcgttc gagcgcgggc ccaccgtacg gcagagagac ggtggcggaa ccagtaaccc
    24061 gcggcacctc ggcgttgcgc caaacttcat tgccaaacgt gtcgtggact accacctcgt
    24121 aaccgtcttc ggaagagtcg tcttcccaga cgaaggtcgg cgtgccctcc atcacctcgg
    24181 cctgctccgc accaggctgc atcacggcga gcgcgcccgt tagcttgaaa gcggccacgc
    24241 tcacctcctt cgagtcgaca tggacatgct ggatcgcggt acccccgatg gaggtgtctg
    24301 gatcccgaac caaggcgtcg ttttcgaacg ccgccagcac gacgtaatcg ccgagaggca
    24361 cgcccgtgaa cgagtaatgc cccgtgacgg ggaactgccg cagcccgatc ggcacttcac
    24421 cgcgggccaa agcctcgttg aacgtcgatt cgaccaccaa gaccacgcta gtctgctgag
    24481 tgcccgcgtt cacgaaattg acgtcaccag tgacggaccc cagtgcagtc gtgctctcct
    24541 tgagcaccac ccccttcacg gctccgccag acggcacctc cgcggctgca ggttcgagct
    24601 gcaagccgga tttgtagcc
//
LOCUS       DASTKU010000143         4535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_27789,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000143 DASTKU010000000
VERSION     DASTKU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27789"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VFQ61_05125"
     CDS             <1..854
                     /locus_tag="VFQ61_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953858.1"
                     /translation="ENVADQPALHSTAEQLREHALRGAELSLRALSQLQNRPDLQPAF
                     ANMKSYGFHVALFDWAYRNSCDPRFLDAVFALADEIASPAGRGGLQITSADQPNHGAY
                     LLSRAAREQGASRLDDQGIKLWALRIAYERRRKPEYVRSAALFMDAWLQQRAEDRLFS
                     GMVRQFDRYLPAGLGEERSPAGHYAILVGLKAWADRLPRARQLYERGFEYTSARADVR
                     RVGYLGPYRMLSRSDGLADFGGEAELGAMLLWAMTGDTHGKSGSKTCTRSVRAPALTD
                     DTRVGAH"
     gene            1012..>4535
                     /locus_tag="VFQ61_05130"
     CDS             1012..>4535
                     /locus_tag="VFQ61_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9953859.1"
                     /translation="MATVQTDEGLTRAPSDVPFAAGVDAPGALRHWMALALESARRSG
                     GPLHVARAEDAVAGGEQAEAWFSWLPDFSATLRHQSGWYRDRGWSEPRWRLDLLARAE
                     LSLTKATALGVAQAEHSRARAALREVEHEVTSELLATSFAMIAAERMITFLDDHLAEL
                     AQVTQGAQQGLHAGPATDAVLLQAEHTRVEAVRTDFVTVLSRARTRLATLTGASIASS
                     RVDPRLAPGELIALSKQLACSKPGEAEGVAEATLTREQRRAEFAAVQSWYVPALRASA
                     VAVFPESNEIDSDSHALRLEQTTGELSLAFTVRPGVPDLRKSSAAAVEGARFDTLSEK
                     KERRLRGARARAQLDVLVPELSTDRALESARSEFRETTRRFARGERKAAEVIASSRRW
                     VEVQLNQDALLNRAIEAQLAECESRRQNDPELHLTDPRVLSPRQVREREVSAPASAPA
                     VLAARARANQALLFARSEDLPWKWGIEAGVLYPLYDRNANTLRARPELGLSGSVLAPV
                     AVNEASLVGRLSFDYLDNARKRKAAQLQAELRSAQAELESRRAASRQLRIRLKLAHAR
                     QMLETRERAAQVAASELELLRNWRSEGFGSDSDVYVSEVAHQAALRARNSARAQLRSL
                     QLELGAELGAAADTALGTEELPAVLEASLSQKHAARGLARFEAALGRRIAELEVAAAR
                     ARLDELTNVPKGLTVLTQATQGLKAGGFSLTAGLGFNLDSPQDSRNALVAAEVLGKAQ
                     ARLRTIDEQLARRYERARLSVSESQATVALERQLQERLAAEQAALTRDGQSWPQASGA
                     NLQRDAIALQLASLASKQREIEAAQSLALASLDLSEYRPSQGAPVVPHTARSNEESSQ
                     IGSLEVNPRKNTSAAPRNARGDIPVVAHWLEAKPGFLAAEGARAQLAAQPTRPAVLGG
                     LRLVGPFAAGSYSSTRVPGPTTFEVAQADLGAGLAFHLDDAVSFLAVSRVRAAAQHAR
                     EAALRAAALQVWSEVAELWRARELHRLAVRREAGARDWLTRWVSPRYELGHLGNHAVA
                     QAEADLTNAAVDLERARARLLAAEAALTTPELEFDMRLLDQYEQAFSTESTSRSDWRQ
                     LEITAPNRSPALRAARERASAQRTQASATSVRVLSPMTGLVEVRPSRTATDTETSEAR
                     RTTSSTERLLEFSVTVPFKPRELGSLIHA"
BASE COUNT          802 a         1464 c         1511 g          758 t
ORIGIN      
        1 cggagaacgt ggcggaccaa cccgcgctgc actcaaccgc cgagcagctc cgcgagcacg
       61 ccctgcgtgg cgcggagctc agcttgcggg cgctcagtca gctgcagaac cggcccgatc
      121 tacagcccgc ctttgccaac atgaagagtt acggctttca cgtcgcgttg ttcgattggg
      181 cctatcgcaa cagttgtgac ccgcggttct tggatgccgt cttcgccttg gccgacgaga
      241 tcgcgtcccc cgccggacgg ggagggctgc agattacgag cgcggatcaa cccaatcatg
      301 gcgcttacct gctctcgcgc gcagcgaggg agcaaggcgc aagccggctc gacgatcagg
      361 ggatcaagct ctgggcgctg cgcatcgcct acgaacgtcg ccggaagcca gagtatgttc
      421 ggtccgcagc attgttcatg gatgcgtggc tccagcaacg cgcggaagat cggctgttct
      481 ccggtatggt gcgccagttc gatcgctacc tgccggcggg tttgggtgag gagcgcagcc
      541 cggccgggca ttacgcgatc ctggtagggc tgaaggcgtg ggccgatcgc ctgccgcgtg
      601 cccgccaact ttacgaacgc ggcttcgagt acacgagcgc ccgtgcggat gtccgccggg
      661 tgggctactt gggcccctac cgcatgctgt ctcgcagcga tgggctagcg gatttcggtg
      721 gagaagcaga gcttggcgcc atgctgcttt gggccatgac cggagatacc cacggcaaat
      781 ctggttcgaa gacctgcacg cgctccgtgc gcgctccggc tttgactgac gacacgcgag
      841 tcggggctca ctgagatgag ctcgatgcgc gcagtccgct cgtcttacat cagtatttct
      901 gccgctctcg tgagcgtgag cgcggctctg acctcgaatg ccgctcgcgc cgagcaggga
      961 atggacggag ccgcgacaga gaattctggg gcgggcgctc ggagtcgttc ggtggcgaca
     1021 gtccaaacgg acgaggggct cacgagagcg cccagtgatg tgcctttcgc cgcgggagtg
     1081 gacgcccctg gcgccctccg acattggatg gcgcttgcct tggagtccgc ccggcggagc
     1141 ggtggtccat tgcacgtagc gcgagcggaa gacgcggtgg caggcgggga gcaagccgag
     1201 gcctggttca gctggctacc cgatttttct gcaactctga ggcatcaatc ggggtggtac
     1261 cgagaccgcg gttggtcgga accccgctgg cgtttggatc tactggcccg cgccgaactg
     1321 tctctcacca aagcgacggc actgggtgtc gctcaagccg aacactcgcg ggcgcgggcg
     1381 gcgctgcgtg aagtcgagca cgaggtcacg agcgagctac tcgcgactag cttcgccatg
     1441 atcgcggcgg agaggatgat cacgtttttg gacgaccacc tcgccgaact tgcacaagtc
     1501 acccagggag cccagcaggg cctccatgcg gggccagcaa cggatgcggt gttgctgcag
     1561 gcggagcaca cgagagtcga ggcggtccgc acggatttcg tgacggtgct ttcgcgagcc
     1621 cgcacgaggc tcgccacgct aacgggcgcg tccatcgcga gcagccgcgt ggacccgcgc
     1681 ttggcgccgg gtgaactgat cgcgctatcg aagcagctgg cgtgttcgaa accgggcgaa
     1741 gccgagggcg tggccgaagc aacgctgacg cgggagcaac gtcgtgccga gttcgccgcc
     1801 gtccaaagct ggtatgtgcc ggcgctacgc gcgagcgcgg tagccgtctt ccccgagtcg
     1861 aacgagatcg actccgattc ccacgctctg cggctggagc aaactacggg ggagctaagc
     1921 ctagcgttca cggtacgccc gggggttccg gatctccgga agagcagcgc cgcggcggta
     1981 gaaggggcgc gcttcgatac gctctcggaa aaaaaggagc gacggttacg gggcgctcgc
     2041 gctcgcgccc aactcgacgt cttggtcccc gagctgagta cggaccgggc cctggagagc
     2101 gcgcgctccg aatttcgcga aaccacgcgt cgtttcgcgc gcggggaacg caaggccgcc
     2161 gaggtcatcg ccagcagccg gcgatgggtg gaggtgcagc tgaaccagga cgcgcttctg
     2221 aatcgagcta tcgaagccca gctcgcagaa tgcgagtctc gacggcagaa cgatcccgaa
     2281 ctccacctga ccgatccacg ggtactctct ccgcgtcaag tgcgggagcg cgaagtctcc
     2341 gcacccgcga gcgctcccgc ggtactcgct gcacgcgcga gggccaatca agctctgctg
     2401 ttcgcccgaa gcgaagatct accttggaaa tggggtatcg aggcgggcgt cctctacccg
     2461 ctctacgatc gaaacgcgaa caccctgcgg gcccgacccg agctcgggct ttcaggctcc
     2521 gttttggcgc cggtggcggt caacgaggcc agcctggttg ggcgcttgtc gttcgactac
     2581 ctcgacaacg cgcgcaagcg caaagcagcc cagctccagg ccgaactccg ctccgcccaa
     2641 gccgagctcg agagccgacg cgcagcctct cgtcagctgc gtattcgttt gaagttggcg
     2701 catgcccggc aaatgctcga aacgcgggag cgcgcggccc aagtagccgc gtccgagctc
     2761 gagttgttgc gcaattggcg ttcggagggc ttcgggtccg atagcgacgt atacgtatcc
     2821 gaagtggctc atcaggctgc gctgcgagca cgcaattcgg cgcgagctca attgcgcagc
     2881 ctgcagctgg aactgggtgc agagctaggg gctgccgcgg acactgcgct cggcaccgaa
     2941 gaacttccgg ccgttctcga agcatctctt tctcagaagc acgcggcgcg cgggctcgct
     3001 cgcttcgaag cggcgctcgg tcgaaggatt gcggagctcg aggtcgcggc tgcgcgcgca
     3061 cgcctcgacg agctcacgaa cgtgcccaaa gggctcacgg ttctaactca agcaactcaa
     3121 gggctgaagg cgggtggctt ttcgctgacc gcggggctgg gattcaatct cgatagtccg
     3181 caggatagcc gaaacgcgct cgttgcggcc gaggtgctgg gtaaggcgca ggcccgcctc
     3241 cggacgatcg acgagcaact cgcgcgtcgt tacgaacgag ctcggctgag tgtgagcgag
     3301 agccaagcca ccgttgcttt ggagcggcaa ctgcaggaac ggcttgcggc cgaacaggca
     3361 gcgttgaccc gagacgggca gagttggccc caagccagtg gtgcgaacct gcagcgggac
     3421 gcgatcgctc tgcagctcgc gtcattggca tcgaaacaac gtgagatcga ggccgcccag
     3481 agcctcgcgc tagcctctct cgatctgagt gaatatcggc catcgcaggg agccccagtt
     3541 gttccccaca cggctcgttc taatgaggag tcatctcaga ttggttctct cgaggtaaat
     3601 ccgaggaaaa acacctcggc tgcgccgcgc aacgcgcgcg gtgacatccc ggtcgttgct
     3661 cactggctcg aggccaaacc tggttttctg gctgccgaag gggcgcgcgc gcagctcgcg
     3721 gcccagccga cccgaccggc agtgcttggc ggtctgcgcc tcgtaggtcc gtttgcggcc
     3781 ggttcatact cgagcacgcg agtccccggg ccaactacct tcgaggtcgc ccaggccgac
     3841 ctcggagcgg gattggcgtt ccatttggac gacgcggtct cattcctggc tgtctcgcgg
     3901 gttcgagctg cggctcagca cgcacgcgag gccgcgctcc gagcggccgc acttcaagta
     3961 tggtccgagg tcgcggagct ctggcgagcc cgcgagctcc atcgcctggc cgttcgtcga
     4021 gaagcgggcg ccagggattg gctcactcgc tgggtaagcc cccgctacga gctcgggcac
     4081 ctaggaaacc acgccgtcgc tcaagccgag gcagatctga cgaacgccgc cgtggatctc
     4141 gaacgggccc gcgcgcggtt actcgcggcc gaagccgccc tcaccacacc cgagctcgag
     4201 ttcgacatga ggctactcga tcaatacgag caagcattct cgacggagtc gacttcccgt
     4261 agcgactggc gacaactcga aattacggca ccgaaccgta gcccggcgct gcgcgccgct
     4321 cgcgaacgcg cgagcgctca gcgcactcag gcctcggcca cgagcgtgcg cgtgctgtcc
     4381 ccgatgactg gtctcgtgga agtcaggcca tcacgtaccg ccacggatac cgagaccagc
     4441 gaagcgcgtc gcaccacctc atctaccgag cggctgctgg agttctcggt gaccgtaccc
     4501 ttcaagcccc gcgagctcgg cagtctcatc cacgc
//
LOCUS       DASTKU010000144         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1142219, whole genome shotgun sequence.
ACCESSION   DASTKU010000144 DASTKU010000000
VERSION     DASTKU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142219"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /locus_tag="VFQ61_05135"
     CDS             complement(<1..1700)
                     /locus_tag="VFQ61_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9953860.1"
                     /translation="MTNSSPWFFLRTALWCVSLVILAPWARAVGPAADTRSLLVAITS
                     EPEEEAYADVRRLWSLWGETDPEAVEAALDHVASSPKVAAPVRAYAQFFLARAQSRRG
                     DLAGAKLEVAKLGYVSEWLVVGPFDNEGKSGLETELGPQSQWDRPLLPGQVYTGKERP
                     VSYRATPKDAFAYGWLDGGALFRPDQNICYFATTFVRTKAKQRVSLWLGNSGAVQLFV
                     DGAEVVEDAAVRGFDTDRHGVLVELVAGVHNVTAKVCGQEDAPAVSLRVARPDGGWPR
                     PDGVTVSAEFENSAEAAKNVGGARVVKSGLGPLSRLQARLQGRKANTRAEDALAKYLV
                     FTISDDPAVHQARDLAKQAAEREPTVERLLFAGELAEDYNQERAWLSRAERLHGDKPP
                     TLDLLLARARVERSGLNWRDAFPHFRDAVALDVRSYPALRGLVELYNEAGLRHTALAA
                     LENALRYEPNAVNVLNMYASQLTALEMHAEADLVEARYAHLRFDDTAYLAARVELGLA
                     QRDRGAVEHWVARLLALSPDNQWAHGVAANAYRQLGQPQRALATYQQALQLAPEDVST
                     "
     gene            2131..>2480
                     /locus_tag="VFQ61_05140"
     CDS             2131..>2480
                     /locus_tag="VFQ61_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET9953861.1"
                     /translation="MVNPQMVMYEEEFNEIQTVVDRLVREANAKVVFIVDKNGQLISA
                     AGDVENIDTTSLASLTAGNIAATGGIAKLLRENEFATQFHEGENANIHIQLVGNRVIL
                     VVIFDSKSSLGLVRL"
BASE COUNT          472 a          802 c          775 g          431 t
ORIGIN      
        1 aacgtgctca cgtcctccgg cgcgagctgc aacgcctgtt gatacgtcgc cagggcgcgc
       61 tgcggctgtc cgagctgccg ataggcattg gccgctacgc cgtgtgccca ctggttgtcg
      121 ggcgacaacg ccagcagccg cgctacccag tgttcgacgg cgccgcggtc gcgctgcgcc
      181 aggcccagct cgacccgagc cgcgagatac gccgtgtcgt cgaagcggag gtgcgcataa
      241 cgcgcttcca ccaggtcggc ctcggcgtgc atctcgaggg cggtgagctg cgaggcgtac
      301 atgttgagta cgttcacggc gttgggttca taacgcagcg cgttctcgag tgcggccaac
      361 gcggtgtgac ggaggccagc ctcgttatag agctccacca accctcgcaa tgcggggtac
      421 gagcggacgt cgagggctac cgcgtcgcga aagtgcggga aggcgtcgcg ccagttcaag
      481 ccgctgcgct cgactcgagc acgcgccagc agcaagtcca aggtcggcgg cttgtccccg
      541 tggaggcgtt cggcccgcga gagccacgcg cgctcctggt tgtagtcttc ggcgagttca
      601 cccgcgaaca acaatcgctc gacggttggt tcgcgttcgg cagcttgttt ggccagatcg
      661 cgagcctgat gaacggcggg gtcatcgctg atcgtaaaca ccaggtactt cgccagcgcg
      721 tcctcggcgc gagtgttcgc cttgcgccct tgcaaccgcg cctgcagtcg cgaaagcggc
      781 cccaacccgg atttcacgac cctcgcgccg cccacgttct tcgcagcctc ggcgctgttt
      841 tcgaactccg cgctgacggt cacgccgtcg gggcgcggcc atccgccgtc ggggcgcgcc
      901 acgcgcaggg atacggctgg agcgtcctct tgaccgcaca ccttggccgt cacgttgtgt
      961 acacctgcga ccaactccac gagcacgccg tgacggtcgg tgtcgaagcc gcgcaccgcc
     1021 gcgtcttcga ccacctccgc cccatcgacg aacagctgca cggcgcccga gttgccgagc
     1081 cacaggctca cccgctgttt ggccttggtg cgcacgaacg tggtggcgaa gtaacagatg
     1141 ttctggtcgg ggcgaaataa cgccccaccg tcgagccaac cgtaggcaaa cgcgtccttg
     1201 ggcgtcgcgc ggtaggagac cggtcgctcc ttgcccgtgt acacctggcc cggcaagagc
     1261 gggcgatccc attggctctg ggggccgagt tcggtctcga gccccgactt gccctcgttg
     1321 tcgaacgggc caaccaccag ccactcggat acataaccga gcttggcgac ctccagcttg
     1381 gcgcccgcca gatcaccgcg ccggctttgg gctcgggcca agaagaactg ggcgtacgcg
     1441 cgcaccggcg ctgctacctt cggggaacta gccacgtggt ccaacgccgc ctcgaccgct
     1501 tccggatccg tctcacccca cagcgaccac aagcggcgta catcggcgta agcctcctcc
     1561 tccggttcgc tcgttattgc cacgagtaac gagcgtgtgt ccgccgctgg gcctactgcg
     1621 cgagcccatg gcgccaaaat caccaaggac acgcaccaaa gtgccgttcg gaggaagaac
     1681 cacgggctag agttagtcat gcgttttcgg ctcttgctgg ggaacgctgt tgttgggcaa
     1741 cacggtgcgt acttaaaggc gcacctacca ggtccgttta atagatgagc tttggagtgc
     1801 tagtgcctgt cgaaacaata agccacctag aacgcgggta caggaaaacc aacgatcctc
     1861 ggccccgcca agccatccca gaggtaccgt ctgggctggc cggaccgcga atgcaagcgc
     1921 agcgctcggc gcgcgcggag aagcacgcct ttttagcagc agtccttcgt ggaatcgctt
     1981 gctggcaagg gcgaatgaaa ggtacaacga agagccttag tgggtcccaa ctcagccgaa
     2041 actcgggggc tctcgaggca accacggcgg aagtgcctag gcgaaatcgc cgtcaaaaga
     2101 ggccggatca cccgctgcgg aggagcgcac atggttaatc cccagatggt gatgtacgaa
     2161 gaggagttca acgagatcca aacggtcgta gaccgtctgg ttcgagaagc gaacgccaag
     2221 gtcgtcttca tcgtagacaa gaatggacag ctcatctccg ccgccgggga tgtagagaac
     2281 atagacacaa cgtcgttggc gtcgctcacg gccggcaaca tcgcagccac cggtggtatc
     2341 gcgaagctgc tacgggagaa cgagttcgcg acccagtttc acgaaggaga aaacgccaac
     2401 atccacatcc agttggttgg aaatcgtgtc atcctagtcg tgatcttcga ctcgaagtcg
     2461 agtttgggat tggttcgcct
//
LOCUS       DASTKU010000145        33327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_27910,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000145 DASTKU010000000
VERSION     DASTKU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 33327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33327
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27910"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..574)
                     /locus_tag="VFQ61_05145"
     CDS             complement(2..574)
                     /locus_tag="VFQ61_05145"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HET9953862.1"
                     /translation="MKAHQPAPRSLRLSLAVGLAALALTGCSKPVPPRVTPLAATVVS
                     VAPSGLRLRVDLDVYNPNAFPLSARSVDGTVSVGSGVVLGQGHAEPSASIAPEQSGTV
                     SSELSIDWQNLAALLPFAASTEPVPYTFDGQATIGGEKLNVTLPFQVKGALSRTDLVN
                     IGLAGMKNSGLPGVLPSGLPNLLPNGVAPR"
     gene            1265..2380
                     /locus_tag="VFQ61_05150"
     CDS             1265..2380
                     /locus_tag="VFQ61_05150"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9953863.1"
                     /translation="MSVGPSSEYARSRFYELDLLRFLAALAVLIFHYAFRGFAADHLT
                     QLPYPWLAQAAKYGHLGVNLFFLISGFVILMTASHGSKRHFLVSRFVRLYPAFWICCT
                     LTFLVTTFAPHFKYVPTWGEYVENLTLFSGFFGIPSVDGAYWSLFVEMKFYVLIFLVL
                     AFGQLRRIKQLLGFWLLATVAATLWPMRYVFFLLIPEYAPYFIAGATFYLVSKEGPSL
                     YKLGLLVSCYALIIIGVVRSVPEFERYYHTSESRVAACAAVSVFYLLFWLIATRPISR
                     KPRKTWLTLGALTYPLYLLHQNIGYVLFNVLYRRLNVHLIFWGTTALMLASAYGVQRM
                     ERVLAKPLKQYLLRLVAQLERLVHSAGIARGPRLKNL"
     gene            complement(2393..3022)
                     /locus_tag="VFQ61_05155"
     CDS             complement(2393..3022)
                     /locus_tag="VFQ61_05155"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9953864.1"
                     /translation="MRVSSQQQYPLIADLQIDILALALACDATRVATLQFDRGAGGPT
                     FNWEGIHHEYNHHKISHGKVRDDCFGSDTSNGCDNVDGYLDMLFDIDRWHQSKFARLL
                     GRLSSYVEADGRSVLDNSVVLYTNELSDGRRHSFTNQPYILAGSAAGVFRQGEQIALG
                     DPSDVYSERAPHNRLLNTLLNAFDVPSDWFGTRSGGRTMQGGIYDALLC"
     gene            complement(3013..3849)
                     /locus_tag="VFQ61_05160"
     CDS             complement(3013..3849)
                     /locus_tag="VFQ61_05160"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9953865.1"
                     /translation="MNELLNHSAGRSSARPIRRRRFLRGIAGAVLGLPFLESLTPRLA
                     RGQADAPVKRLAVFFCCNGVNVPRWFPKLVSGPLLDEHLLGTANEPLLPFRDKLLFPR
                     GIHMAPRGYDFDGGGADDHGKGMAHKLTAQFADPEKWLALGPSLDHVLATHINPGLEG
                     ARRPPLNLMVGRFSGERSLDYISYSEARTPVAAMNSPWTAYAELTGLTRGAAPTPEAE
                     ELVIRRRQSVIDLVSEQFADLRRMNLSLEDDRKLEQHKAASPIGCRLTLPVGTVRPIS
                     CA"
     gene            complement(3846..5537)
                     /locus_tag="VFQ61_05165"
     CDS             complement(3846..5537)
                     /locus_tag="VFQ61_05165"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9953866.1"
                     /translation="MDPRDPMPPIRLFIVSAFSLPACTLSSSASFDNSGQGPDFATPT
                     PAASVRETGFARLNHREYENTVVDLLGLGRGIASGFAPDANRGSRFDTDADLRVDSRL
                     GPQYRIAAEGLAEQLVADDAAFARAVPCEPAEAACSDRFIAEFGLKALRRPLEPQELR
                     RFKQLFERGSELYAGSDAFRDGVRIVVEAMLQTPQFIYRTALSAQPGARLSSWELASK
                     LAYFVHGSMPDPELRELAGRDALQTDEEVRAAVVRMIEAPRALSQFVTFHEQALDVAR
                     FARIQPDPAQYPELPGDYSMRVRAATSAFFVTLVEERGGVRELLSAPFAFADAALAPL
                     YGVEWPGAAATRDFSSDTELRRIELPGRAGILMQIGFLAANAHAIATDPIHRGLFVLR
                     DILCREVPDPPPGASQSPLPAGAPPPRTTRESVSTLTAGASCSGCHSQINPVGFAFEQ
                     FDAVGRFRELENGVTIDASGSMELDGQPLQFADAGTLVEALANSEEARRCYARHWLEF
                     ASGRAISGSDDPAVTAQLDELSQSGLSIRDIALRIAGAAEFAQRAIPVVVPKAEP"
     gene            5883..6836
                     /locus_tag="VFQ61_05170"
     CDS             5883..6836
                     /locus_tag="VFQ61_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET9953867.1"
                     /translation="MKPRHSADYRTILWMLVFAPAVMAAQFVNPELIGKLTILSLYFG
                     VSAAVIAHNHNHSPTFTSKRANSWFGKVLSIFYGYPTFAWIPTHNLNHHKLVNKPGDA
                     TITWRFTNRHNVLVAATYFFVSAWFQRIPTSQFIERAKAQNPKLHAMIRGQFWFWGLA
                     HAGLLSLAIFLHGPLLGLKVWFFAVFVPALFALWTVHLFNYEQHVHTDPWSEYDHSRN
                     FISPLLNFLLFNNGYHTAHHENAGLHWSQLPKLDAELAPRINPALREKSVWWYWFRQY
                     VLAVFVPKLGTTQIGRAPFDTPNLDLRTDDVEAATYNASRA"
     gene            6847..7443
                     /locus_tag="VFQ61_05175"
     CDS             6847..7443
                     /locus_tag="VFQ61_05175"
                     /inference="COORDINATES: protein motif:HMM:NF025244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HET9953868.1"
                     /translation="MDDVRFSHAVAAFDRISTEDPALDAAGRPKELVQKEHLVRWVLA
                     LRPAPSDALLLAAHCQHVGRHKTPRSSYPEGRAGYLRWRTESSRRHADIAEGVLAQEG
                     FEPDLIRRVRAIVEKRNLGRDEEVQVMEDALCLAFLETEFAEFAERHEDDKLLVILRK
                     TWKKMSEEARQRALQLPFSGRAATLLTQALTPESSPPA"
     gene            complement(7447..9606)
                     /locus_tag="VFQ61_05180"
     CDS             complement(7447..9606)
                     /locus_tag="VFQ61_05180"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9953869.1"
                     /translation="MYHPYGETSAQEEHEFVELFNAGKEPALLSGYKLRVDGRDRLSL
                     PANLSLGPGQYLVLAKSASALLSISSYMLEPSRVIGDYSGELDNGGAEVALVSARGNV
                     LDSVTYDDAAPWPIGADAFGADEEFLPALAPFDAHRFRGRSLERVDLTGPSSAPATWE
                     ASAIDAATPGRANAATRTAPIALTLTVVAAGSSSGTISPTTPAQIEVTLSGPPSEVPV
                     VEYRVDVLGLPGSSSASLSLEGVPAAAGVYRAILPAAPANSVVRYRIKAGPASDAARI
                     SPRLGDPNEWHSYFVEPPTEGTQFTSSAQRLFIAPGDWTSLWTNLDGGQNMSCTINAK
                     WDAEVPAVFVASGKVYDVRVRYQGSRNRRKDGLEIENFPAAGPSQPDPLLALSFHVSF
                     PNYAEYDGENSINLNKLKQACPGVLNALEGALFDQVGVPAQNFRFTRVYVNGAYYTYS
                     MEVRGIDARTMRKYEGKGNPIGLLFKADGDNSNGPWGLANFTPLNAACGAPVSDRYLR
                     SYELHTHKELIAGGQSHTELVSLIEELNRLQANSDANPEVRAFFERNFDVSELATQFA
                     IRNWAGVWDDGFHNYYLYKRPSDGKWLILPQDFDCDFGGDPVQCGDFGKFYNAPTLSF
                     FHPERSGGVTAVATSRLKIQFIRAFRAEFSARVIELGKTIFSESNIDSTLTRVLSAFD
                     LAAWNAAPTRYCDVNARVQQAKEFLAKRRAFLAEGIK"
     gene            complement(10133..10705)
                     /locus_tag="VFQ61_05185"
     CDS             complement(10133..10705)
                     /locus_tag="VFQ61_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953870.1"
                     /translation="MSTVQKIQVKLLSSVEPDVIPYVPVFHRWIRDNALSELLIDVVD
                     YSHVQNGPEVLLVGHGADYALDREGGHLGLLFANKRDDLGENGFATALRRAFRAAALL
                     ESESGPERPILFRADELHFKVADRLSTPNDDASFRRLEPSLKAALSALYPGLPFELLR
                     TGTSRELFSVQARISGAPAFSEAAKRAAAL"
     gene            complement(10717..12972)
                     /locus_tag="VFQ61_05190"
     CDS             complement(10717..12972)
                     /locus_tag="VFQ61_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HET9953871.1"
                     /translation="MTAPTWKAALQGKIREDWAREIDIYETQIELRKKGKIEEKLFAE
                     TRLRRGVYGQRYDNGQRNDGEKTQTLQYPCGDLTKGPVTVWDAPGMQRIKVPMGRLSP
                     EQLDVMCDLAEEYSDQILHVTTRQDIQLHFVHIEDTPDLMRRLAAVGITTREACGNSV
                     RNVTACPYAGVCSDESFDVLPYADAMMFFLLGHDDTQDFGRKFKIAFSGCGDNPCGLT
                     NFHDLGCIAKVREVDGVRQRGFEVYVGGGLGAVPQMAQLLDDFVPEAELLPLTQAVCR
                     VFGRLGEKENRARARIKFLVKKLGIEEFKRLTLEERQKLRPDPRWTAFLDEINRPGAK
                     PNLAAAQLTQSPADARFTAWRSSNVRPQRQPGYVTAKVTLPLGDFTPTQGRRIADIAR
                     RYGEDSLRTTVEQNLLFRWVPEAELPALYRELSEIGLGDPGADTITDVTSCPGTDTCK
                     LGISSSRGLAAELRRHLTVLEAKSELDVAAKGLNIKCSGCFNSCGQHHIADLGFLGVS
                     RNVGGRRVPHFQLVVGGELTGNARSYGLAIGAIPSKRVPAVVDCLTRTYVEKKLPDES
                     FKGFVDRIGKKEVKKLVDQFTSVPSYEEDPSFYSDFGDPREYTIGDMGVGECAGEVVP
                     FTQFGLAASEREVFEAQVLLDEGKLKEAGDRAYSAMLQAARALTRQHNQNLGEDPAEI
                     IAEFKTHLVDTKVFDPLAGAKFAQFLFRAHTEGSKRADNKENAHQLIEEAQLFVDAAY
                     QAYSRVGARTA"
     gene            13348..14544
                     /locus_tag="VFQ61_05195"
     CDS             13348..14544
                     /locus_tag="VFQ61_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9953872.1"
                     /translation="MSLPKSTFANSTDAVHAGVPKTRSQHTLTASIAQTATYTFEDTE
                     SLERYMRGEDPDPHREEYGRYGNPTVREFETRVAELERAEDAVAFSSGMAAVTSTLFA
                     LLKSTDHVVLFKDCYRRTRQFVTQQLPRFGIEYSVIPSGDLAALQGAMKDNTRLVLTE
                     SPTNPFQYCIDLAALSRTVKARGRIRTLIDSTLATPCNSRPIELGIDIVMHSATKYLS
                     GHNDVLGGVVAGPSHLLSLVREMRNVLGGVLDPHAAFLLLRGLKTLGLRVERQNQTAL
                     AVARALEGHPSIERVFYAGLESHPSYGIAKQQMNGGGGMVSFIVRGGRAAASAVVDRC
                     KLAKIAVSFGGAETLIDQPAIMSYFELSEEELKRIDMDPALIRLSVGIEETEDVVRSV
                     LEALPR"
     gene            complement(14660..17332)
                     /locus_tag="VFQ61_05200"
     CDS             complement(14660..17332)
                     /locus_tag="VFQ61_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HET9953873.1"
                     /translation="MLRNLAIRLKLLLLAGVPVVGALILAVLLARGARREAATAEALG
                     SIEDLARLSAQMSTLVHEVGTERNASALALASETPDAAALDRVAKQSDLAYQGLSGFL
                     RARRMTALPPRLARDLNEAQQKFASIGEIRSKVREKQTGIVEVLDYYNAINHSLVSAT
                     AALAQLSDDGEMMRIISSLVGVLQLQETESKEHALLSHVFAAKTFPPGIYKELVTLVT
                     EQHDHVAQLELAASDNAAARFRSLRRGPEFERAIALRKLALDTLDDDFKVEASEWVRV
                     REDGLDRLRSLEIELNREVAARAVAKLEAARRQLRLSYGIGGSIILFSLLLATLIGRG
                     IHRSIGNLASAAQRVRSEKDFSVRAVKLSDDELGSLTEVFNEMLSGIQERDSELSEHR
                     QNLERLVEVRTRELQRRNESMRMVLDNVEQGLATIQPNAVLASERSRAFDAWFCPDPE
                     RAPSDSGLECIAKGNPDIEAALRLGWEQIQDGLLPLEVALDQMPKQIAVQGRHYALNY
                     RPITEQDKLIGVLLVVSDITDDLLRARREAEQRELIAVFERVMRDRSGFIEFFQECES
                     LVEQVVHGRINDVSHVMRAVHTVKGNCALFGVTSVAEVAHELESDMTERGSMPSADQI
                     KRLGEAWQALSDRVRRLSGEEAEPILEIAHEELGQLVDAAKQRVPHERLIALLERLKY
                     ERAVVRLRRSAEQAQALAKRLDKGDLKVEINAGDVRLPAERWASFWAAFVHVIRNAVD
                     HGIEPIAEREATGKPPYGTLRLSTSSNGRELFIELHDDGRGIDWDRVRERAKERGIPH
                     ANDGDLAQALFEDGLSTAQRVTDVSGRGVGLGAVRDVTSALGGSIAVLSKRGEGTTFR
                     FTFPLSLALKSNPSSRYPGARPSLTFPNSERGVN"
     gene            17533..18759
                     /locus_tag="VFQ61_05205"
     CDS             17533..18759
                     /locus_tag="VFQ61_05205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9953874.1"
                     /translation="MSAPTSIGLVVPNFNGERFLTATLNSIAEQHYPQLRCIVMDGGS
                     RDASVSIARRFQERHPWLEVVSEKDEGQADAIDKGVRRLDTELVGWLNSDDLLLPGTL
                     HAIADARQRNRGGALFYGDLELCDDSGRLFSIMPALDLNYDTLRRGRGRILQPGSFYT
                     RALLQKAGGVRKEFWGLMDLDLWIRLLEQGEAVRLQRRVAQFRVHPAAKSSEQPLRYY
                     RESLRVAWRYNPDRRVRALLPKLRTVPLHFLRWLFDVHVNAYTPPPLISLPAQIELEG
                     ALMPNAVADSLKAAPEFAGAFSATPVATPDIRVLHADTLLGQPRPGRINQSRPRIPVI
                     ACASGLSERQASALTALREPLAFVLTTPAAMDRLRCAGFPDFRVGDRTLASDWLRALR
                     VASGELFPSGDNSAGS"
     gene            complement(18794..19252)
                     /locus_tag="VFQ61_05210"
     CDS             complement(18794..19252)
                     /locus_tag="VFQ61_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953875.1"
                     /translation="MSVGALLWGACALLPGCGSKGAVSLSARIDNAQLTVQDATLGTK
                     LSGKFDLVLELGEAAPRATEVQLGAFSLKNDHGVLVERLALESEPFPISVAVGTTRSV
                     PFTLDDSELVTPGSAEICAGPVYIGGSVSDSLSNGKASNVTSSMLAIDCP"
     gene            19656..21122
                     /locus_tag="VFQ61_05215"
     CDS             19656..21122
                     /locus_tag="VFQ61_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953876.1"
                     /translation="MLARSGEAPAPDVSLGPAALPSSISSGVSSLPALALSQSGPLSF
                     ESSSPDGRWVVVCQAREDTNGDSKLEVKVQPNGDLRGDALRRYLVLASGTELAIEDAW
                     LSSPAGNHLIVKRAGHVELLDLERGSSLELEPLAADLRREPSAREGHRSLALTSDSLL
                     YVKLAGSSSALIERGLKEGSERVLWQGPESVVRFTLEGGGRYVVLWVPGRDVNGNGRF
                     DWPFKPTLDAKPCRGSVPRFVSSEIGPDAQSAIVIDRYRSETMRFDDLAVVLADGVVR
                     RDPEGALFLQRLGERASQLADKDCAARVLWSERTDQSLLFGCVLPKKPTRLGVEAYTR
                     GRRQSLDLDVAWLGLDEPPAREPTRLVSLYPGADTILFDTEKQTLHRLNAGDGVLGTW
                     GPHALVRRGRTLLIFDAEAHREALLPGSLESFGSVFVTGSLVYASPLLVDVAQGRVLG
                     QIAARPLALTRGGQLLIPQIPATDEHLAEGPLAWKNAE"
     gene            21138..22409
                     /gene="tyrS"
                     /locus_tag="VFQ61_05220"
     CDS             21138..22409
                     /gene="tyrS"
                     /locus_tag="VFQ61_05220"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET9953877.1"
                     /translation="MQLLEDLAFRNLIYQQTNRDGLLEHLATPRRVYCGFDPTAPSLT
                     IGNLVPILLLARFQRAGHIPVVIMGGGTGLIGDPSGKSAERPMLSAEDVEHNVQNQRT
                     IFERVLDFSGAHGARLLNNASWLGKIGYLEMLRDVGKYFSVNMMIQKDSVRERLENRE
                     QGISYTEFSYMLLQAYDFLHLHDTEGVTVQVAGSDQWGNVVAGVDLIRRVRRVEAFGQ
                     TAPLVTKSDGTKFGKSESGAIWLSANRTSPYEFYQFWLNTSDADVESYLKIFTFIEPA
                     RITELCEEHRANPGARAAQRALARHVTELLHGADALAQAEAASAALFSGNVTGLSRET
                     LSELFKHAPRATVAKAGLEGQGILLVDLLIQTQVAKSKREAREFLDNKAIQLNGELAN
                     LDTRVTTNSLLYGESLLIRRGKKLWYVINVE"
     gene            complement(22585..24639)
                     /locus_tag="VFQ61_05225"
     CDS             complement(22585..24639)
                     /locus_tag="VFQ61_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9953878.1"
                     /translation="MRTALTGRLLSHVGSALVVLGLLGTSRQALAQTPVDRRVPEQNG
                     AGMDTHLFRPAVDSKGFFSVNGSDIIPRNAISFGLVLDYGRNLLRTRRDDIPVGQSPG
                     QPAGQPCKEDSNECTVQGAGTGVPALISNSFQGTFSANYGLANFGVVGISLPVILMTG
                     DTAYQIGPNSALYNSGDLNAQKIGALNLHGKFRFTRVDRTIGLGAVIQAGIPLGSAPR
                     DMGWDPGFSLWPQLIAENRFGSTGRFRVGVNVGYRMHSGKNPRFQNDVTGRPQLDEGT
                     LRYGNLGTFGVGLSLRALDALDIVAETYGSYLTSSGTDSKQKLSQEVVGGIKLFVEQN
                     SYLMMGAGSRAFSTGFEAADLRLILGFVFEPSIGDRDGDGYKDDQDSCPDEPEDFDGF
                     QDGDGCPEPDNDNDGILDVDDRCPNVPEDHDGDQDEDGCPEGKLDGDRDGDGIPDSKD
                     KCPDVPEDRDGYQDEDGCPEADNDKDGIPDTEDQCPMDAEDRDKFEDQDGCPDPDNDR
                     DQIPDVKDKCPNDPETYNGYQDEDGCPDKGKVVIEGNDIIIFEKVQFETNSAKILPQS
                     NEILNAVAAVLKGHPEFQVVEVGGHADERGSDSHNLQLTKARAASVMEALRSRGIATN
                     RLLSQGYGEYCPLDDRSTPAAWDKNRRVEFKVVRTEEGDTGVKRGCERASEGGIYPPK
                     PR"
     gene            25209..26708
                     /locus_tag="VFQ61_05230"
     CDS             25209..26708
                     /locus_tag="VFQ61_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9953879.1"
                     /translation="MSLTTLAHAQSVTLAGRWSASALRTDWNVGDWGSACGERPSGAG
                     AASAGAGAGSVTIRVSGKELSMAFPGRAFSTAECWEQLPGMVRESHSAGARSYRTVCK
                     SQAADPRRAVIITTISATDNQIQFDETGQYQFVIQNQNCTASVRRTRVLTLLQREGDP
                     APAASPAASAARPAPSLPSPTKPAPRCQTTGLPERMEVRPSHKLARPGESFAFRSAVL
                     DAAGCSLPIMPTFRIVKGGALGELSGAGKLKIFDQAEEGELTLEATVGDRSVQVRVQV
                     VSQERYDALLKQGDFNEQGESAEAASTRIASSSIGARSGVARDEARGRRMAFLAIVGA
                     LSLVLGCAGLFILVRTRRARPKRSISREELASAITGPDHFPDAQLASLRPTSRVCPTC
                     REEYPPHAEFCANDGNRLLPVVAGAGVSANSIVAPTGGVCPICGQGYDPGVNVCPKHQ
                     EPLVPVTVWAERRRRENTLIRKICPVCGTQFSGDSQFCGKCGAALVPVN"
     gene            26940..28523
                     /locus_tag="VFQ61_05235"
     CDS             26940..28523
                     /locus_tag="VFQ61_05235"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET9953880.1"
                     /translation="MKITCDSCGAKYTIADDKVRGRKVKIRCKGCSAPIVVDGQQPEG
                     GMRIEGGSTDASGTPSVSSVPPGGAWSVNLSETDQQTLTTEQIVQGWAGGQVTADAFV
                     WRQGMADWLPILECAELAPLLNGTAAAPAAALAPAPAKSDSLVPAGGRAAVSARVVGG
                     RAQNTMDLFAGVDKAGADGVHDESEDVVMPGAGPALPNRPQTAEEARPTGARNENSVL
                     FSLDALKAGVTTGGKPEPQKPSRAPQRGVVAEDPFNLSGGGVAALHGGPMFSLADNQA
                     LLTAPPPPEPPKVVQSFATGAPTPQAKSSSKGGLVIGAIVLLLAGAGGAVLLMGGKKE
                     VAENKTTSSEIQKKDESAAASETKEEPKKEEAKAEEPKATESEKKPEETASKEEKPAD
                     GKAAETKPEEGTKAVASTKAGTSGSTASTKPKKEEPKEEAKPAGGDQPFSKSAAIASL
                     GAAANQAGSCKKLGGPTGSGKVTVTFASSGKVTSANVSGPPFAGTSVGGCVASIFRKA
                     HVPPFSGSSVTVSKGFTIN"
     gene            28759..29430
                     /locus_tag="VFQ61_05240"
     CDS             28759..29430
                     /locus_tag="VFQ61_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9953881.1"
                     /translation="MSSLIELQKVRKSYRSGQEVLPILRDVDLSIGEGEFVSIMGSSG
                     SGKSTLLNILGILDRYDSGTYYLDGRLIRDLSETEAARYRNRFIGFVFQAFNLLPFKT
                     AAENVALPLYYQGVSRKKRLELSREYLERVGLAARAEHLPNQLSGGEKQRVAVARALV
                     TRPKLLLADEPTGALDTRTSYQLMELFQALNESGITLVIVTHEHDIAERTRRVIRLRD
                     GRVED"
     gene            29437..30672
                     /locus_tag="VFQ61_05245"
     CDS             29437..30672
                     /locus_tag="VFQ61_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9953882.1"
                     /translation="MLLEGLLEIAQTLWQHKLRTFLTALSVAWGTFMLSLLLGLGQGL
                     RTGAQWEFRDNAVNSLWFFNSTTSLSYRGRGPGRSVYFNNEDLDVLGRRLPEVQYRSG
                     RYYFWGEFFVSYGAKHASFDIRGVHPEHRYIARTQMLQGRFVNDIDLREHRKVAVIGS
                     KVRESLFEKEDPIGKQIQIRGLTYQVVGEFADEGGEAELRRIYVPLTTTQLVYNQPNI
                     IHNLILTLKSDDLAESSRVAQQARAILGDRHDVAPEDRRAIRVQNNLEQFAKTMNIFV
                     WLGVFVWIVGAGTLLAGVVSVSNIMLISVRERTKEIGIRKALGATPRSIVGLIVTEAM
                     LLTSVSGYLGLFGGVFLIEAIADRTQGVPFMRQPHVDLRSVLIGAGLLVVAGALSGLF
                     PALKAARVDPIVALREGDT"
     gene            30669..31916
                     /locus_tag="VFQ61_05250"
     CDS             30669..31916
                     /locus_tag="VFQ61_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9953883.1"
                     /translation="MNLDSWQELLHTLSANKLRAFLTACGVFWGIFMLVFMLGMGTGL
                     ERGVRRSLGNMTSRAVYVWSQRTSLPYRGLQPGRYLKFHNADAEALRRVPGVVYVAPR
                     LQLGAWRAGTNAVYKGRTGNFVVLGDSPEFFRVEPSTLKRGRVFNPRDQAEVRKVAVI
                     GDQARHVLFGEEDPIGKYISVRGVYLMIIGEISSEKSGDDGERIRSSLFVPLSTFQAA
                     FNQRDRIGWFTLTAREDVDPEQLEQRVKATLRGRHGVHPEDRDALGSFNLAAQINKVN
                     GLFRGIQSFVWFVGTMTLLAGVLGVGNILLITVKERTREFGVRKALGATPSSVVNMVL
                     REALLLTSMAGYTGLVASVGGLELMARVVAKLQNAPIERPEVDLRVALIATLVLIIAG
                     TLAGLVPAVHAARIRPVEALRSE"
     gene            31913..32992
                     /locus_tag="VFQ61_05255"
     CDS             31913..32992
                     /locus_tag="VFQ61_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9953884.1"
                     /translation="MIRRLLPLLFAALVLVGFGWTLVFLYKKSEAKPAAFRSVSPERR
                     TIVKKTVAPGAIVPRREVTIKPRVSGVIEKLYVQPGQYIKEKALVAKIQIIPNMVSLN
                     NAEANLRSAQINLDTAQREFERFEKLKTQGLTTETEFVQRELAFKLRQGERDAAENNL
                     QLVRVGASKQSGKVSNVVLATLDGMVLEVPVKEGGSVIEANNFNEGTTIAALADMRDM
                     IFQGRVDESDVGRVKAGMPVTINVGALGEERFSGTLEYIAPKGVEKEGTIQFEVRAAV
                     QLKQDTFLRANYSANADIILDRKENVLSIAEADVTFESGQTFVEVEDGPQHFVRRKVE
                     LGLSDGIYTEVKSGLTESARIKKHE"
     gene            33043..>33327
                     /locus_tag="VFQ61_05260"
     CDS             33043..>33327
                     /locus_tag="VFQ61_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953885.1"
                     /translation="MKRSENHWYILEILAKSIARSYRLNRVRIGFWALGIVLCLTAIQ
                     TDAMRTLLSSADTSRALSLAVPAALLVVFLGIVQVRQRAQRQAYWSALREP"
BASE COUNT         6204 a        10536 c        10417 g         6170 t
ORIGIN      
        1 actacctcgg cgcaacgcca ttcgggagca ggttcggtaa cccactcggt aggaccccgg
       61 gtagccccga gttcttcatt ccggcaagcc cgatattgac gagatcggtg cgagacaagg
      121 cgccctttac ttggaacggc agcgtgacgt tcaacttctc gccacctatc gtggcttgac
      181 cgtcgaatgt atatggcact ggttcggtac tcgccgcgaa cggcaagagc gccgccaagt
      241 tctgccaatc gatgctcagt tcactgctca cagttccgga ctgctcgggc gcgatgctgg
      301 cactcggttc ggcatgtcct tgcccaagca ccacgcccga acccacgctt acggtaccgt
      361 ccacagagcg ggcagagagc gggaacgcat tcggattata gacgtccaag tccactctga
      421 gcctcagtcc actcggagca actgaaacca cggtcgccgc aagtggggtc acgcgcggcg
      481 gcaccggctt cgagcaaccc gtcaaagcca acgccgccaa gccgaccgct aaactcaaac
      541 gcaatgagcg aggtgccggc tgatgggctt tcatccgcga gaccctacca caaccgtgag
      601 cttcgcccga gaagcgtatc gttacggtac gcgagagctc ttgccggata ggccgccgcc
      661 caaaccaaac caagccagcc aagccagccg agccccagcc gaccaagccc aaccgagcca
      721 ccgcaaagcg acgctctgca accaacttgc agttcacggt ctgacgcgcc gcggtcacga
      781 aacaacgtcc gctcgatacc aggactcgag ttgaccggac ggatcgctgc ctcgcgcatc
      841 cgccccggcg gcacttagag ccgacaaccc ataacctcga gaaaaccgac cactcgcggg
      901 tgcaacacaa cgcgaagaca acggagcgca agccacacag cgcgagccac acagcgcgag
      961 ccacacagcg cgagccacaa actagtcgaa tagcgactct gcaggtgggt cacaaaatgc
     1021 ctatccgccg ttcccacaaa gcttgtaaat gagggatcgc tcggaccgga gttttcgatt
     1081 gccacctctc ggaagcctga gctccgcctg tgcgccactg gctgggtcaa acatggacgc
     1141 caactttttg aagccaccat atcgaacgac atcatcgatg ccctcgggcg tcacggaggg
     1201 tcgtccaagt cttgctccca atagtgtggg cccggtgagc gtgggcccgg tgagtgcggg
     1261 cccgatgagc gtgggtccca gcagcgagta cgctcgttcc cggttttatg agctcgattt
     1321 gcttcgcttt ctggcggcgc tcgccgtgct catttttcat tatgcatttc gcggcttcgc
     1381 cgccgatcac ctcacgcagc ttccctatcc gtggctcgcg caggctgcca agtatgggca
     1441 cctgggcgtg aacttgttct tcttgatcag cggtttcgtg atcttgatga ctgcgtctca
     1501 cggatccaaa cgccacttcc tggtgtcacg cttcgtgcgc ttgtaccccg ccttctggat
     1561 ttgctgcacg ctcaccttcc tcgtcacaac cttcgctccg cacttcaaat acgtgccgac
     1621 ttggggcgag tacgtcgaga atctcaccct attcagtggc ttcttcggga ttccctcggt
     1681 ggacggcgcc tattggtcgc tgttcgtcga gatgaagttt tacgtactca tcttcttggt
     1741 gctcgccttc gggcagcttc ggcgcatcaa gcagctgctc gggttttggc tcttggcaac
     1801 cgttgccgca actctctggc cgatgaggta cgtgttcttt cttctaatcc cggagtacgc
     1861 accgtacttc atcgcaggcg caaccttcta cttggtctcc aaggagggcc cctcgctcta
     1921 caagctgggc cttctggtca gctgctatgc gctgatcatc atcggcgtcg tgcggagtgt
     1981 ccccgagttc gaacgctatt atcatactag cgagagccga gtcgcggcgt gtgcagcggt
     2041 gtccgtgttc tatcttctgt tctggttgat cgcgacacgc ccaatcagcc gcaagccgcg
     2101 caaaacgtgg ctcaccctcg gagccttgac ctatccgctc tatttactgc atcagaacat
     2161 tggttatgtg ctgttcaacg tgctttatcg acgcctgaac gtgcatttga ttttctgggg
     2221 caccaccgcc ttgatgctcg cctccgcgta cggagtgcag cggatggagc gcgtattggc
     2281 gaagcctctg aagcaatacc tattgcgctt ggtcgcgcaa ctcgaacgtc tcgttcatag
     2341 cgcagggatc gcgcgcggcc ctcgcctgaa aaatctgtga gcccgaccgc actcaacaca
     2401 gcaatgcatc gtaaataccg ccttgcatgg tacggccacc gctgcgcgta ccaaaccagt
     2461 cggatggcac gtcgaaggca ttgagcagcg tgttcagcaa gcgattgtgc ggagcacgtt
     2521 cgctgtagac gtcactcggg tccccgagcg cgatctgctc gccctgcctg aacacccccg
     2581 cggcgctgcc cgcgagaatg tagggctggt tcgtgaacga gtgtcgcctg ccgtcggaga
     2641 gctcgttcgt gtaaagcacc acgctgttgt cgagcacgct gcgaccatcg gcttccacgt
     2701 acgaggagag ccgaccgagc aggcgcgcaa atttggactg atgccaacga tcgatatcga
     2761 acaacatatc cagatatcca tcgacattgt cgcagccgtt cgaagtatcg ctgccgaaac
     2821 agtcgtcccg caccttgccg tgcgaaatct tgtgatgatt gtactcgtgg tgaatgccct
     2881 cccaattgaa ggtggggccg cccgcgccgc gatcgaactg gagagtggcg acgcgcgtcg
     2941 cgtcacacgc cagcgcgagc gccaaaatgt cgatttgcag atcagcgatc agcggatact
     3001 gctgctgcga actcacgcgc acgagatcgg cctgactgtc cccactggga gcgtaagccg
     3061 acacccgatc ggtgatgctg ccttgtgctg ctcgagtttg cggtcgtctt cgagcgagag
     3121 attcatgcgg cgcaaatcgg cgaactgttc agaaaccaag tcgatcacgc tctgccgacg
     3181 tcgaatcacg agttcctcgg cctcgggggt gggagcggct ccgcgcgtca acccggtcag
     3241 ctcggcatac gcggtccacg ggctgttcat ggccgccacg ggcgtgcgcg cctcactata
     3301 ggaaatgtag tcgaggctcc gctcgccgga gaacctgccg accatgaggt tgagtggcgg
     3361 gcggcgcgcg ccttcgagtc caggattgat gtgagtggcc aatacgtgat cgagggacgg
     3421 gccgagcgcg agccattttt ccggatcggc gaactgagcc gtgagcttgt gggccatacc
     3481 cttgccgtgg tcgtccgcgc cacctccgtc gaagtcgtag ccgcgaggcg ccatatgaat
     3541 gcctcgtgga aagagcaact tatcccgaaa aggcaacaac ggctcgttgg cagtgcccag
     3601 caggtgctcg tcgagcagcg gtccggaaac gagctttgga aaccagcgcg ggacgttcac
     3661 tccgttgcag caaaaaaaca cggcgaggcg cttaaccggc gcatcggctt gcccccgcgc
     3721 cagtcgcggc gtgagcgatt cgagaaacgg taagccaagc acggcgccgg cgatcccgcg
     3781 caagaagcga cgccgcctga ttggtcgggc ggagctacgc cccgcagagt gattcagaag
     3841 ctcgttcatg gttctgcttt cggcactact accgggattg cgcgctgcgc gaactcggcc
     3901 gcaccggcta tgcgcagcgc gatgtctcgt atggatagac cggactgtga taactcgtcg
     3961 agctgcgccg tgactgcggg gtcgtccgat ccggagatgg cacgaccgct cgcaaactcc
     4021 agccaatgcc gcgcgtagca gcgtcgtgct tcttcgctat tcgcgagcgc ctcgacgagc
     4081 gtgcccgcgt cggcgaactg gagcggctgg ccgtcgagct ccatgctccc actcgcgtcg
     4141 atggtaacgc cgttttccag ctctcgaaat cgccccacgg catcgaactg ctcgaacgcg
     4201 aatccaaccg ggttaatctg agagtgacaa ccactgcagc tcgcccccgc cgtcagagtc
     4261 gatacggatt cgcgcgtggt acggggcgga ggcgctcccg cgggtaaagg gctctgtgac
     4321 gcccccggag gcggatccgg gacctctcga cacaggatgt cccgcaacac gaacaatccc
     4381 cggtggatcg ggtcggttgc gatcgcgtgc gcgttggccg ccaggaaccc aatctgcatc
     4441 aggatcccag ctcgccctgg gagctcgatt cgccggagtt ccgtgtcact cgagaagtcc
     4501 cgtgttgccg ctgcgccggg ccactccact ccgtagagag gagcaagcgc cgcatccgcg
     4561 aaagcgaaag gtgcggaaag taactcgcgc actccgccgc gctcttccac aagggtgacg
     4621 aagaacgcgg acgtggccgc gcgcacgcgc atcgagtaat caccgggcaa ctccggatat
     4681 tgcgccgggt caggttggat gcgagcaaag cgcgcgacgt cgagcgcctg ctcgtggaaa
     4741 gtcacgaact gagagagcgc gcggggcgct tcgatcatgc gcacgactgc cgcacgcact
     4801 tcttcgtcag tctggagcgc atctcgcccg gcgagttcgc gcagttccgg gtccggcatc
     4861 gaaccgtgga cgaagtaagc cagcttgctg gcgagctccc agctgctcag acgggccccg
     4921 ggttgagcgc tgagcgccgt gcgataaatg aactgtggtg tttgcagcat ggcctcgacc
     4981 acgatgcgca cgccatctcg gaacgcatcg gagcccgcgt acagctccga cccgcgttcg
     5041 aacagctgtt tgaagcgccg caactcctgc ggctccaacg gtcgacgcag ggccttgaga
     5101 ccgaactcgg cgatgaagcg gtccgagcag gcagcttccg ctggctcaca gggcactgcg
     5161 cgcgcaaagg cggcgtcgtc cgcgaccagt tgttctgcca aaccttcggc cgcgatgcgg
     5221 tattgcggtc cgagtcgaga gtccacccgc agatcggcgt ccgtatcgaa acgggagccg
     5281 cgattcgcgt cgggggcgaa gccgctcgca atcccccgac ccagacccag caggtccact
     5341 accgtgttct cgtattcgcg gtggttgaga cgtgcgaagc cggtttcgcg gacgctagca
     5401 gcaggggttg gagtagcgaa gtccggaccc tggccgctgt tgtcgaaact tgcgctcgaa
     5461 ctcaacgtgc acgcaggcag agagaacgcc gagacaatga agagccggat gggtggcatt
     5521 gggtcccgag gatccatgtc gcgaccagtg cgcgttgagc cgtcaaatgt cagcttcgtt
     5581 caaacacctt cgctgggctg ccttgacgac tggtaatagg aatgccaacg aacggggggc
     5641 cgcgcgccac tggagaggga gctttgttcc ctgctcggac attctttcgg gccggcctcc
     5701 ccgcacgctt gcaatttcga acgaaagctc caaagagcca tagcttcgaa gccctggctc
     5761 tctggccctg gcaccaaaga gcagctcatt gagctgccca cacgaagccg cctcgaagtt
     5821 tgacgctcag caccaaagcc gctcgctgac tccgccccgc tcaccaagtt ggaaagccaa
     5881 gcatgaagcc ccgacacagc gccgactatc gcacgatcct gtggatgctc gtgttcgcgc
     5941 ctgccgtgat ggccgcgcag ttcgtcaatc ccgagctgat cggcaaacta acgatcttga
     6001 gtctttactt cggtgtgtcg gccgccgtca ttgcccacaa tcacaatcac agccccacct
     6061 tcacgagtaa gcgtgcgaat tcgtggttcg gcaaggtgtt gtcgatattc tacgggtacc
     6121 ccacgttcgc ctggatcccg acgcacaacc tgaatcatca caagctcgtg aacaagccgg
     6181 gtgatgcgac gattacctgg cgtttcacca atcgacacaa cgtgctggtg gccgcgacgt
     6241 acttcttcgt ctcggcttgg ttccaacgta tccccaccag ccagttcatc gagcgcgcca
     6301 aagcgcagaa ccccaagctg catgcgatga ttcgcgggca attctggttc tggggcttgg
     6361 cccacgccgg cttgctgagc ctcgcgatct tcctgcatgg cccgctgctc ggactgaagg
     6421 tctggttctt cgccgtgttc gtgccggcgt tgttcgcgct ctggacggtc cacctgttca
     6481 attacgagca gcacgtccac accgatcctt ggtcggagta cgatcactca cgcaacttca
     6541 tcagcccgtt gttgaacttt ctgctcttca acaatgggta ccacaccgcc caccacgaga
     6601 atgcgggctt gcactggagc cagctgccca agctcgacgc ggagctcgcg ccgcgcatca
     6661 atccggcgct gcgcgagaag agcgtatggt ggtactggtt ccggcagtac gttctcgcgg
     6721 tgttcgttcc gaaactcgga accactcaga ttggccgcgc gccgttcgat accccgaacc
     6781 tcgacctgcg aacggacgac gtggaggccg ccacttacaa cgcgagccgt gcttgaatag
     6841 cgaccgatgg atgacgtccg gttttctcac gcagtagccg ccttcgatcg aatcagcacg
     6901 gaagatccag ctctagatgc ggccggtcgc cccaaggagc tcgttcagaa agagcatttg
     6961 gtgcgttggg tgcttgcgtt gcggccagcc ccctcggacg cgctgctcct cgcggcccat
     7021 tgccagcacg tcggtcggca caagacccct cgttccagtt acccggaggg ccgcgccggt
     7081 tacctgcgct ggcgcacgga gtcgtcccgg cggcacgcag atatcgccga gggggtactg
     7141 gcgcaggagg ggttcgagcc cgatttgatc cgtcgggtga gggccatcgt cgagaagcgc
     7201 aacctcggtc gcgacgagga agttcaggtc atggaggacg ccctatgttt ggccttcctg
     7261 gagactgagt tcgccgagtt cgccgagcgg cacgaggacg acaagctgct cgtgatcctg
     7321 cggaagacct ggaaaaagat gtcggaagag gcacgtcagc gcgccctgca gctgcctttc
     7381 agcgggcgtg cggctacgct gctgacgcaa gcgctgacgc ccgagagctc cccgcccgcc
     7441 tgatccttac ttgatgccct ccgccaagaa cgcgcggcgc ttggccaaga attccttggc
     7501 ttgctgcacc cgcgcgttca cgtcgcaata gcgcgtgggc gcggcgttcc aagccgcgag
     7561 atcgaaagcg gacaaaacgc gggtcagcgt cgagtcgatg ttggactcac taaaaatggt
     7621 cttcccgagc tcgatcacgc gcgccgagaa ctccgcgcgg aaagcccgga tgaactgaat
     7681 cttcaagcgc gaggtcgcaa ccgccgtcac cccaccgctc cgctcgggat gaaagaacga
     7741 gagcgttggc gcattgtaga acttcccgaa gtctccgcac tgcacgggat ccccgccaaa
     7801 atcgcaatcg aagtcttgcg gcaggatcag ccatttcccg tcactcggac gtttgtacag
     7861 atagtagttg tggaagccgt cgtcccagac gccggcccaa ttgcggatcg caaactgagt
     7921 ggccagctcg gacacgtcga agttgcgctc gaagaacgct cgtacctctg gattggcgtc
     7981 gctgtttgcc tgaagacggt tgagctcctc gatcagcgac accaactcgg tgtggctctg
     8041 accccccgcg atcagttcct tgtgggtgtg cagctcgtag ctgcgcagat agcgatcgga
     8101 aacgggagca ccgcaggccg cgttcaaagg cgtgaagttc gcgagccccc aggggccatt
     8161 gctgttgtcc ccgtcggctt tgaagaggag cccgatcggg ttgcccttgc cttcgtactt
     8221 ccgcatggta cgcgcgtcga tgccgcgcac ttccatggag taggtgtagt acgcgccgtt
     8281 cacgtagacg cgtgtgaaac ggaagttctg ggcgggaact ccgacctgat cgaacaaggc
     8341 cccctctagc gcgttgagta cccccggaca ggcctgcttc agcttgttca agttgatcga
     8401 gttctctcca tcgtactccg cgtagttcgg aaaggagacg tggaacgaga gcgcgagcag
     8461 tgggtcgggt tgcgagggac ccgccgcggg aaagttctcg atctcgaggc catctttgcg
     8521 gcggttgcgg ctgccctgat agcgcaccct cacgtcgtag actttgccgc tcgccacgaa
     8581 caccgccgga acctcggcat cccacttggc gttgatggtg cagctcatat tctgcccgcc
     8641 gtcgaggttg gtccacaggc tcgtccaatc gccgggggca atgaacaggc gttgagcgga
     8701 gctggtgaac tgggttcctt ccgtgggcgg ctcgacgaaa taggagtgcc actcgttggg
     8761 gtcaccgagg cgcggactga tccgagccgc gtccgatgca ggacccgcct tgatccgata
     8821 gcgaaccaca ctgttggccg gcgccgctgg caatatcgcg cggtaaaccc cggctgcagc
     8881 gggcacaccc tccaatgaaa gcgacgcgct gctcgagccg ggcaatccca acacgtccac
     8941 gcggtattcg accaccggca cctcgctggg tggcccggaa agcgtaactt caatctgagc
     9001 cggcgtggtg ggcgagatcg tcccgctaga gctgccggcc gcaacgaccg taagggtcag
     9061 cgcgatagga gcagtccgcg tcgccgcatt cgctcgcccc ggcgtcgctg cgtcgatcgc
     9121 cgaggcttcc caagtagcgg gtgcggagct cgggcccgtc agatccactc gctcgagcga
     9181 acgccctcga aaccggtgtg cgtcgaaggg agccagcgcg ggcaagaact cctcgtctgc
     9241 tccgaatgca tccgccccaa tcggccacgg cgcggcgtcg tcgtaggtaa ccgagtccaa
     9301 gacgttaccg cgtgcgctca cgagcgcgac ttcggcccct ccgttgtcga gctctccgct
     9361 gtagtctccg atcacgcggc tcggctcgag catgtaactc gagatcgaga gcaaggccga
     9421 cgcgctcttg gccagcacca ggtactggcc ggggccgagg ctcaggttgg caggtaagct
     9481 cagccggtct cggccgtcga cgcgcaactt gtaaccggag agcagcgcgg gctccttgcc
     9541 cgcgttgaat agctccacga actcgtgctc ttcctgagcg ctcgtctccc catacgggtg
     9601 gtacatgatt tccgagatca cgacctctgg cagtgtctcg ggagcactcg attgggagct
     9661 gctacaagaa gcactcgcga gtgccaaaag ggtcgttgcg gcggaggcga gcggttgacg
     9721 aagataacgc aaggaggcca aacccttcag agtgtgccac gccgtgcgcg atgcgagccg
     9781 gggcccggac aaatcgcgtg accgcgcggg gcgactagga agtcacctct cccgcgctcc
     9841 gtcattctga cgcgcggtct caattctcgc gtgcccgctc tttttgcccg ctcgccgttc
     9901 ggcacgttta tcaaccgtat tttcttgacc gagcctgccg tctcatcgcc ctcacgggtt
     9961 acgggaccgc cgagccctcc gcgcaaacgc catgctcggt ggagacatct cgccagaagt
    10021 acgtggcagc tatcgcccac ctgacgagct tcgccacgag cagggcgagc ggcccttccg
    10081 ccgcccgccc cttctcaatg gccgccctgt tccgccacta ggccacgacc gctcagagtg
    10141 ccgcggcgcg cttggcggcc tcggagaacg ctggcgcgcc agagatgcgg gcctgaacag
    10201 agaacagctc gcgcgaggtt cccgtgcgca acaactcgaa aggcaaaccg ggatacagcg
    10261 ccgacagcgc cgccttcaac gacggctcca agcggcgaaa cgaagcgtcg tcgttcgggg
    10321 tgctgagccg atcggcaacc ttgaaatgca gctcgtccgc acggaaaaga atcggcctct
    10381 cggggccgct ctccgattcc agcaacgcgg ccgccctgaa cgcccgacgc agcgccgtag
    10441 cgaagccgtt ctcacccaag tcgtcgcgct tgttggcgaa caacaagccc aggtgcccgc
    10501 cctcgcggtc gagggcgtaa tccgctccat gcccgaccag cagcacctcg gggccattct
    10561 ggacgtgcga atagtcgacg acgtcaatca acagctcgga cagcgcgttg tcgcggatcc
    10621 aacggtgaaa aaccgggacg taagggatca cgtccggctc gaccgaggag aggagtttga
    10681 cctgaatctt ctgcacggta ctcatggttt tgccgttcaa gcagtgcggg cgccgacgcg
    10741 cgaataggcc tgatatgccg cgtccacgaa gagctgagct tcctcgatca gctggtgggc
    10801 gttctctttg ttgtcggccc gcttggagcc ctcggtgtgg gctcggaaca ggaactgagc
    10861 gaacttcgcg cccgccaacg gatcgaacac cttggtatcg acgaggtggg tcttgaactc
    10921 cgcgatgatc tcggccgggt cttcaccgag gttctgattg tgttggcggg tcaacgctcg
    10981 ggccgcttgc agcatcgcgc tgtacgcgcg atccccggct tctttgagct tgccttcatc
    11041 gagcagcacc tgagcttcga acacctcgcg ctcgctcgcg gcgagcccga attgcgtgaa
    11101 aggcaccacc tcaccggcgc actcgcccac gcccatgtcc ccgatcgtgt actcgcgcgg
    11161 atcaccgaag tccgagtaga agctcggatc ttcctcgtag ctcggcacgc tcgtgaactg
    11221 atcgaccagc ttcttgacct ctttcttgcc gatgcggtct acgaagccct tgaagctctc
    11281 gtccggcagc ttcttctcta cgtaagtgcg ggtcaggcaa tccacgacgg ccggtacgcg
    11341 cttggacgga atcgcgccga tggccaagcc ataggaacga gcgttgccgg tcagctcgcc
    11401 accgaccacc agctggaagt gcggcacgcg ccggcctccg acgttgcggc tgacgcccaa
    11461 gaatcccaag tccgcgatgt gatgttggcc gcaggaattg aagcatccgc tgcacttgat
    11521 gttcagaccc ttggcggcga catcgagctc ggatttggcc tcgagcacgg tcaagtgacg
    11581 gcgcaattcg gcggccaatc cgcgcgacga cgagatcccc agtttgcagg tatccgtgcc
    11641 ggggcaagag gtcacatcgg taatggtgtc ggcgccggga tcaccgaggc caatctcact
    11701 cagctcgcga tagagcgccg gcaactcggc ctccggcacc cagcggaaca acaagttctg
    11761 ctcgacggtg gtgcgcagcg aatcctcgcc gtaacgacgg gcgatgtcgg cgatgcgacg
    11821 accctgcgtg ggagtgaaat cgccgagcgg cagagtaact ttcgccgtca cgtaacccgg
    11881 ttgacgctgc gggcgcacgt tgctgcttcg ccaggcggta aagcgcgcgt ctgcgggcga
    11941 ttgagtgagc tgcgccgcgg ccagattggg cttggcaccg gggcgattga tttcgtcgag
    12001 gaacgcggtc cagcgggggt cggggcgcag cttctggcgc tcttcgaggg tgagtcgctt
    12061 gaactcttcg atcccgagct tcttcaccag gaatttgatt cgagcgcgcg cgcggttctc
    12121 tttttctccg aggcgtccga acacgcggca gaccgcctgc gtcaacggca gcagctcggc
    12181 ctcgggcacg aagtcgtcca acagctgggc catttgaggg actgcgccga gcccaccacc
    12241 cacgtaaacc tcgaagccgc gctgccgcac gccgtccact tcgcgcacct tggcgatgca
    12301 gccgagatcg tggaagttcg tgagcccgca gggattgtct ccacagcccg agaacgcaat
    12361 cttgaatttg cgcccgaagt cctgcgtgtc gtcgtgcccg agcaggaaga acatcatggc
    12421 gtcggcgtaa ggcagcacgt cgaaggactc gtcgctgcaa acgcccgcgt aagggcacgc
    12481 ggtgacattg cggacggagt tgccgcaagc ttcgcgcgtc gtgatcccga ctgccgccag
    12541 gcgtcgcatc aagtccggcg tgtcctcgat gtgaacgaag tggagctgga tgtcctggcg
    12601 cgtggtgacg tgcaggatct ggtcgctgta ctcctcggcc aaatcacaca tcacgtcgag
    12661 ctgctcgggg ctcagccgtc ccatcggtac tttgatgcgc tgcatgccag gcgcatccca
    12721 aaccgtgacc gggcccttcg tcagatcgcc gcatgggtac tgcagggtct gcgtcttctc
    12781 tccgtcgttg cgctgaccgt tgtcatagcg ctgaccgtac acaccgcggc gcaagcgtgt
    12841 ttcagcgaac agcttctcct cgatcttgcc cttcttccgg agctcgatct gggtctcgta
    12901 gatgtcgatc tcgcgagccc agtcctcccg aatcttgcct tgtaacgctg ctttccaggt
    12961 cggggcggtc atctctgcca atgctccact cttttctatc gcaggccttc aaacgcaggc
    13021 ttctctaagc gcagactgtc gaaacggaga gactctaaaa cgcaggacct tcgaacgcag
    13081 aactttcgac gcggaacctt cgaacgcgga atcgttcatg tgccggtgtt tttcgagtcc
    13141 gagtcgaggg ttatacccgt gattcccggt gcgacaagtc gagaatccct ccaggggcgg
    13201 cgctcctcct tgaaacgacc gaaagattta gcctttttca gatcgtatac ttgacctatt
    13261 cagtcaagaa ttagaccgtc tcggaatgac gagcgcccac gtggggcgac aactcggcac
    13321 cccgctgtta tacgtggcga gcccgagatg tccttgccca agagtacctt cgcaaactcc
    13381 accgacgccg tgcacgcagg tgttccgaag acgcgttcgc agcacacgct gaccgcgtcc
    13441 atcgcgcaga cggccaccta tacgttcgag gataccgaga gcctcgagcg ttacatgcga
    13501 ggggaagatc cggatccaca tcgcgaggaa tacggccgct acggtaaccc aaccgtgcgg
    13561 gagttcgaaa cccgcgtcgc agagctcgag cgcgccgaag acgccgtcgc gttctcgagc
    13621 ggtatggccg ccgtcaccag cacgttgttc gcgctcctca agagcacgga ccatgtcgtg
    13681 ttgttcaaag attgctaccg gcgcacacgc caattcgtga cccaacagct gccgcgcttc
    13741 ggcatcgagt acagcgtgat cccgtcgggg gacttggcgg ccctgcaagg cgcaatgaag
    13801 gacaacactc ggctggtgtt gaccgaatcg ccgacaaacc cgtttcagta ctgcatcgat
    13861 ctggcagcgc tctcgcgtac cgtcaaagcg cgcggccgaa tccgcacgtt gatcgactcg
    13921 acgctggcca ccccctgcaa ctcgcgtccg atcgagctcg gcatcgatat cgtcatgcac
    13981 agcgcgacga agtacctctc cggacacaat gacgtgctcg gcggggtcgt tgccgggccg
    14041 agccacttgc tcagcctcgt gcgtgagatg cgcaacgtgc tcgggggagt cctggatcct
    14101 cacgctgcgt tcttgctgct gcgaggtttg aagaccttgg gcttgcgggt cgagcggcag
    14161 aaccagaccg cgctggcggt ggcgcgtgcc ctcgaggggc acccgagcat cgagcgggta
    14221 ttctacgcag gcctggagag ccacccttcg tatggcatcg cgaaacaaca gatgaacggt
    14281 ggcggcggca tggtgagctt catcgtgcgc gggggccgcg ccgccgcttc tgcggtcgtc
    14341 gatcgctgca agctcgccaa aattgcggtg tcttttggtg gtgcggagac gctgatcgat
    14401 cagcccgcga tcatgagcta cttcgagctc tcggaagagg agctcaagcg aatcgacatg
    14461 gatcccgcat tgattcgtct gtcggtaggg atcgaggaaa ccgaggacgt ggtccgctcg
    14521 gtgctcgaag cactgccccg ctaagagcca ccttcgaagt cctgctgaac cggaagctga
    14581 aacggccggg tcgacttgcg cagaatgcgc ggaatcgacc cggtacaccc ttcagttcaa
    14641 acagttcggt tcaaacagtt cagttcacac cccgctccga gttcggaaag gtcaggctcg
    14701 gacgggcgcc agggtagcgc gagctcggat tggacttcag agcaagcgac agagggaacg
    14761 tgaagcggaa ggtcgtaccc tccccgcgct tcgacagcac tgcgatgctc cccccgagcg
    14821 cgctggtgac gtcccgcacc gcaccgagcc ccacgcctcg cccggataca tcggtgactc
    14881 gctgagcggt gctcaagcca tcttcgaaca aggcctgcgc gagatcgcca tcgttcgcat
    14941 gggggattcc tcgctctttc gcgcgttctc gcacgcgatc ccaatcgatg ccacgcccgt
    15001 cgtcgtgcag ctcgatgaac agctcgcggc cgttgctgga ggtcgagagc cgcagcgtgc
    15061 cgtagggtgg cttgcccgtg gcctcgcgct ccgcgatggg ctcgatgcca tggtcgaccg
    15121 cgttgcggat cacgtggacg aaagccgccc agaacgaagc ccagcgctct gccggcaagc
    15181 ggacgtcccc cgcattgatc tcgaccttga gatcgccctt gtccagccgc ttggcgagcg
    15241 cctgagcctg ctccgccgag cgacgcaggc gcaccactgc gcgttcgtac ttcaggcgct
    15301 cgaggagcgc gatcaggcgt tcgtggggca cgcgctgctt ggccgcgtcc acgagttggc
    15361 cgagctcctc gtgcgcgatt tccaggatcg gctctgcctc ttcacccgac agtcgtcgca
    15421 cgcgatcgga gagggcctgc cacgcctcac caagcctctt gatctggtcc gcggagggca
    15481 tgctgccccg ctcggtcatg tcgctctcga gctcgtgtgc gacctcggcc accgaagtga
    15541 cgccgaatag agcgcagttg cctttgaccg tgtgcaccgc acgcatcacg tgcgagacat
    15601 cgttgatccg accgtgcacc acctgctcca cgagcgactc gcactcttgg aagaactcga
    15661 tgaagccgct gcgatcgcgc atcacgcgct cgaacacggc gatcagctcg cgctgctcgg
    15721 cctcacgccg ggctcgcagc agatcatcgg tgatgtcgct caccaccagg agcaccccaa
    15781 tcagcttgtc ttgctcggtg atcggccggt aattgagcgc gtagtggcgc ccttgaaccg
    15841 cgatttgttt gggcatctga tcgagcgcga cctcgagcgg caacaagccg tcctgaatct
    15901 gttcccagcc caggcgcagc gccgcctcga tgtcggggtt acccttcgcg atgcactcca
    15961 ggccggagtc gctgggcgcc cgctccggat ccgggcaaaa ccaggcgtcg aacgcgcgtg
    16021 accgctcgct cgccagaacg gcgttgggtt gaatggtggc caacccctgc tcgacgttgt
    16081 cgagcaccat ccgcatggac tcgttacggc gttgtagctc acgcgtacgc acctcgacca
    16141 agcgctcgag attctgacgg tgctcgctga gctcgctatc ccgctcttga atcccggaca
    16201 acatctcgtt gaacacctcg gtcaacgagc cgagctcgtc atcggagagc ttcaccgcgc
    16261 gaacgctgaa gtccttttca ctcctgacgc gctgggcggc ggacgccagg ttgccaatcg
    16321 aacgatgaat gccgcgccca atcagagtcg cgagcagcag agaaaacagg atgatgctgc
    16381 caccaatgcc gtacgacagg cgaagctgcc ggcgcgcggc ctcgagcttg gccacggcgc
    16441 gcgcggcgac ctcgcgattg agctcgattt ccaagctgcg cagacgatcg agtccgtctt
    16501 ctcgaactcg cacccactcg cttgcttcca ctttgaagtc gtcatccaac gtatcgaggg
    16561 cgagcttgcg caaggcgatc gcccgctcga actcgggacc ccgacgtagg gaacgaaagc
    16621 gcgccgccgc gttgtcagag gcggccagct ccaattgcgc aacgtggtcg tgttgctcgg
    16681 tcaccagcgt gaccagctcc ttgtaaatac cgggcggaaa cgtcttggcg gcaaatacgt
    16741 gactgagcag cgcgtgctct ttgctctcgg tttcttggag ctgaagcacc ccgaccaacg
    16801 aggagatgat gcgcatcatc tcgccgtcgt cgctgagctg cgcgagcgcc gcggttgcgc
    16861 tgaccaagct gtgattgatg gcgttgtagt agtcgaggac ctcgacgatg cccgtttgct
    16921 tctcgcgcac cttgctgcga atttctccga tggacgcgaa tttttgctgc gcctcgttca
    16981 gatcacgcgc gaggcgcggc ggcagcgcgg tcatccggcg ggcccgcaag aagcccgaga
    17041 gcccctggta ggcaaggtca ctttgcttgg cgacccgatc caaggcggcc gcgtcgggcg
    17101 tctcgctggc gagcgcgagc gcggacgcat tgcgctccgt gcccacctcg tggacgagtg
    17161 tactcatttg cgccgagagc cgcgccaggt cttcgatcga gcccagggcc tcggcagtcg
    17221 cggcttcgcg acgcgcgccg cgtgccagca agacggcaag aatcagggca cccaccacgg
    17281 gcacccccgc gagcaggagg agcttcagac gaatggctag gtttcgcaac atagggtgac
    17341 gtggaacccc acagaatcca gggtttctaa aggcagtcgg ttggtagacg gcacggcgac
    17401 gcctcgagtt gagtctgtgg caaatgagcc ctcagcggcg cgagacgctc accacgaact
    17461 tacgcgagcc gacacatcca gacggcgtgg ctggcttgac ccctacaaac cgcctggact
    17521 agtcgtgtgc gggtgagtgc tcccacttcg atcggcctgg tcgtccccaa ctttaatggg
    17581 gagcgattcc tgacggccac cttgaacagc atcgccgaac agcactatcc gcagctgcgc
    17641 tgcatcgtga tggatggcgg atcccgagat gcatcggtgt cgatcgctcg acggttccaa
    17701 gaacgccatc catggctcga ggtagtcagc gaaaaggacg agggtcaggc ggacgccatc
    17761 gacaaaggtg tgcggcgttt ggatacggaa ctcgtcggct ggctcaactc cgacgacttg
    17821 ttgctaccag ggacgctgca tgcgatcgcg gacgcgcgtc agcgcaatcg aggtggcgcg
    17881 ctgttctatg gtgatctcga gctgtgcgac gactccggtc gtctattctc gatcatgccc
    17941 gcgctggatc tgaactacga tacgctgcgg cgcggtcggg gccgcatcct gcaacctggc
    18001 tcgttctaca cgcgggcctt gctacagaaa gccggcggag tacgaaaaga gttctggggg
    18061 ttgatggatt tggacttgtg gattcgcctg ctcgagcagg gcgaggcggt gcggctgcag
    18121 cggcgcgtag cccagtttcg agtacatccg gcggcgaaga gcagtgaaca gccgctgcgc
    18181 tactaccggg agtccctgcg cgtggcctgg cgctacaacc ccgatcgacg tgtgcgcgcg
    18241 cttctgccga agctgcggac cgtgccgctc cattttttgc gctggctgtt cgatgttcac
    18301 gtcaacgcat acacgccgcc gccgctcatt tcattgcccg cgcagatcga gctggagggc
    18361 gcgctgatgc ccaacgccgt cgccgattcg ctcaaggccg cgccggagtt cgccggagcc
    18421 ttctccgcta ccccggtcgc caccccggac atacgtgtgc tccacgcgga cacattgctc
    18481 ggccagccac ggcctggtcg aatcaaccag agccgacccc gaattcccgt tatcgcctgc
    18541 gcgagtggac tgagtgagcg gcaagcgtcc gcgctgaccg cgttgcggga gccgttggct
    18601 ttcgtgctca ccacacccgc cgcgatggac cgcctgcgat gcgcgggctt cccggatttc
    18661 agagtcggtg atcgcacgct agcctcggat tggctgcgcg ccttgcgggt ggcatctggc
    18721 gagctcttcc cgtcaggcga caattcggcc ggttcctgaa tcgcagcgct agcgttcgac
    18781 cggtctgacg cgctcagggg caatcgatgg cgagcatcga gctcgttacg ttgctagctt
    18841 ttccattgct cagagagtca ctgacgctgc cgccgatgta gaccggtccg gcgcagatct
    18901 ctgcggagcc cggcgtaacg agctccgaat catccagcgt gaaaggcacg cttcgcgtcg
    18961 taccgaccgc gacgctgatc gggaatggct cggactcgag cgcgagccgt tctacgagca
    19021 cgccgtgatc gttcttgagc gagaatgccc cgagctgcac ctcggtcgct ctcggagccg
    19081 cctcgcctag ctcgagcacc agatcgaact tgccggagag cttggttccg agcgtcgcgt
    19141 cttgcaccgt gagctgcgcg ttgtcgatac gtgcgctcag agatacggcc cctttgctac
    19201 cgcaacccgg gagcagcgcg catgcccccc aaagcagtgc gcctacgctc aagggccgaa
    19261 gccacgcctc cccatgacgc tcgcgcgaag aaccgggctc gggggaggca gtcgaacgga
    19321 acgaaagcgc taaattcgag tggctttggc gcatggcaag ctcgggcgta cgggcacccg
    19381 gaacatagca tcgtgaggct gggattccga gcggtgcccc gcgacttccg gcacaaagct
    19441 catgcggctt gagttagtgt gcgcacacgt gcgtgctcga tcgatctttg tttccgccaa
    19501 tcacgctgcc gaatagcttg gcgccaaaaa actccacgcc tgtcgtaagg ctgttcggag
    19561 cctttctttt ggctcagttc gcgtgggcgt gtggggcgtc ctcgtcgcaa cgagcatcct
    19621 cattcgcgcc accctcaccg ccacctcctt tcacgatgct agcgcgatcg ggggaagctc
    19681 ccgcacctga cgtgtcgctc ggccctgcag cgctacccag ttcgatctcg agtggcgtgt
    19741 cgtcgctgcc ggctttggca ctgtcgcaga gcgggccgct cagcttcgaa tctagctcgc
    19801 cggatgggcg ctgggtggtg gtgtgtcaag cgcgcgaaga cacgaacggt gactccaagc
    19861 tcgaagtaaa agtgcagccc aatggcgacc tgcgcggtga tgcactgagg cgctatctcg
    19921 tgcttgcctc gggtacggag ctcgctatcg aagacgcttg gctgagctcg cccgcgggca
    19981 atcacctgat cgtaaaacgg gcgggtcacg tcgagttgtt ggacctggaa cggggaagca
    20041 gcctcgagct cgagccgctg gccgccgatc tgcggcgtga gcccagcgcg cgtgaagggc
    20101 accgcagctt ggcgctcacc tcggattcac tgctctacgt gaagctcgcc ggctcgagca
    20161 gcgcgttgat cgagcgcggc ttgaaggaag gcagcgagcg cgtgctctgg caaggcccgg
    20221 agagcgtcgt acggttcact ctggagggtg gtggcaggta tgtcgtgctg tgggtgccgg
    20281 ggcgcgacgt caacggcaac ggccggttcg actggccttt caagccaacc ctggacgcga
    20341 agccgtgccg aggcagcgtg ccgagattcg tcagttcaga gatcggcccc gacgcgcaga
    20401 gcgcgatcgt gatcgaccgc tatcgctcgg aaaccatgcg cttcgacgat ctggcggtag
    20461 tgctggcgga cggagtggtg cggcgggacc cggaaggcgc gttgtttctc caacggcttg
    20521 gcgaacgggc cagtcagctc gctgacaagg attgcgccgc gcgcgtgctg tggtcggaaa
    20581 gaaccgatca atcgctgttg ttcgggtgcg tcttgcccaa gaagccgacg cggctcgggg
    20641 tggaagcgta tacgcggggg cgtcgacaaa gcctggatct cgatgtcgcc tggcttggct
    20701 tggacgagcc acccgcgcgc gagcccaccc ggttggtctc actctatccg ggtgcggata
    20761 cgatcctgtt cgataccgaa aagcagacac tccaccggct caacgccggc gacggagtgc
    20821 tcggcacctg gggcccgcac gcgctcgtgc gacgcggccg tacgctgttg atattcgacg
    20881 ccgaggcgca ccgcgaggcc cttctacccg ggagcctcga gtcgttcggt agcgtgttcg
    20941 tgacgggcag cctggtgtac gcgagcccat tgctggtcga cgttgcgcag ggccgcgtac
    21001 tgggacaaat cgcggcacgc ccgctcgcgc tcacccgtgg tgggcaattg ttgattccgc
    21061 aaatcccagc caccgacgag catctagcgg aggggccgct cgcctggaag aacgccgagt
    21121 agtatacagg cgcgacgatg cagctcctgg aagatttggc gtttcgaaac ctcatctacc
    21181 agcagaccaa ccgagacggc ctgctggagc acctcgcgac tcctcgccgc gtctattgcg
    21241 gtttcgaccc cacggcgccg tctctcacga tcggcaactt agtgccaatc ttgttgttgg
    21301 cgcggtttca acgcgccggg catatcccgg tcgtgatcat gggcggcggc acggggttaa
    21361 tcggtgatcc ttccggcaag agcgccgaac gcccgatgct gtccgccgag gacgtggaac
    21421 acaacgttca gaaccagcgc accatcttcg agcgcgtcct cgacttctcg ggagcccacg
    21481 gcgcgcgcct gctgaacaat gcgagctggc tcggcaagat tggttacctc gagatgctgc
    21541 gcgacgtggg caagtacttc tcggtcaaca tgatgatcca gaaggactcc gtccgggagc
    21601 gcctagaaaa tcgcgagcaa ggcatcagct acaccgagtt cagctacatg ctgctccagg
    21661 cttacgactt cctccacctc cacgacaccg aaggggtcac ggtacaggtc gcgggctccg
    21721 atcagtgggg caacgtggtc gccggcgtcg atttgattcg ccgcgtgcgg cgcgtggagg
    21781 ccttcgggca aacggcgccg ctcgtcacca agtcagatgg taccaagttc gggaaatccg
    21841 agtctggcgc gatctggttg agcgcaaacc gaacctcgcc gtacgagttt taccaattct
    21901 ggctgaacac gtcggatgcg gatgtcgaga gttatctgaa gatcttcacg ttcatcgagc
    21961 cggcgcgaat tacggagctc tgtgaggagc accgcgcgaa cccgggagcg cgagccgcgc
    22021 agcgcgcgct ggcccgacac gtgaccgagc tcctgcacgg agctgacgcg ctcgctcaag
    22081 cagaagcggc atcggcggca ttgttctccg gcaacgtgac gggcttgagt cgtgagacct
    22141 tgagtgagct cttcaagcac gcgccacgcg ctaccgtcgc caaggccggc ctggagggcc
    22201 agggcatctt gctcgtggat ctgttgatcc agacgcaggt tgccaagagc aagcgtgaag
    22261 cgcgcgagtt cctcgacaat aaagccatcc aactgaatgg ggagctcgcc aatctcgaca
    22321 cccgcgtgac gaccaactcg ctgctgtacg gagaaagtct attgatccgc cgcggcaaaa
    22381 agctctggta cgtgatcaac gtcgagtagg cgctcgcgaa cccagctgct ctgtggcgcc
    22441 accaaaggcg gatagagagg gcagccgtca ggtcacggac aaggaaaagg ccgaagcaac
    22501 tggtttgctt cggcctttcg cgtttgcgtg ttctctcggt tcgggtgagc cctgcctcat
    22561 gcggcaaggc ccaccgaaac tgcgtcagcg aggcttgggc gggtagattc cgccttcgct
    22621 tgcgcgctca caaccacgct ttacgcccgt gtcaccctct tcggtacgca cgaccttgaa
    22681 ctctacgcga cgattcttgt cccacgcggc gggagtcgag cgatcgtcga gtgggcaata
    22741 ctcaccgtag ccttgtgaca acaagcgatt ggtcgcgatg cctcgcgagc gcagcgcttc
    22801 catcaccgac gccgcgcgcg ccttggtgag ctgcaggttg tgcgagtcgg acccgcgctc
    22861 gtccgcgtga ccaccgactt ctacgacctg gaactcggga tgccctttca gcacggccgc
    22921 gaccgcattc aagatctcgt tcgactgcgg caggatcttc gcgctgttgg tctcgaactg
    22981 caccttctcg aagatgatga tgtcgttgcc ttcgatgacg accttgccct tgtccgggca
    23041 gccatcttcg tcttgatagc cgttgtaggt ctccggatcg ttcgggcact tatccttcac
    23101 gtcggggatt tggtcgcgat cgttgtccgg gtccgggcac ccgtcctgat cttcgaactt
    23161 gtcgcgatcc tcggcgtcca tcggacattg atcttcggtg tccgggatgc cgtccttgtc
    23221 gttgtcagcc tcggggcagc cgtcttcgtc ctgataaccg tcgcggtctt cgggaacgtc
    23281 ggggcacttg tccttactgt cggggatgcc gtcgccgtcc cgatcgccgt cgagtttgcc
    23341 ttcagggcag ccgtcttcgt cctgatcgcc gtcgtgatct tccggtacgt tcgggcagcg
    23401 gtcgtcgaca tccaggatgc cgtcgttgtc gttgtccggc tcggggcagc catcgccgtc
    23461 ttggaacccg tcgaagtctt cgggctcgtc cgggcaggag tcttgatcgt ccttgtagcc
    23521 gtccccgtcc cggtcaccaa tcgaaggctc gaacacgaaa ccgaggatca gtcgcagatc
    23581 tgcggcttcg aagccggtcg aaaatgcgcg actgcccgcg cccatcatca agtaactgtt
    23641 ctgttccacg aacagcttga tcccgcccac gacctcctgg ctgagcttct gcttgctgtc
    23701 cgtgccgctc gaagtcaggt aggaaccgta ggtttcagcg acgatatcca gagcatccag
    23761 cgcgcgcagc gagaggccca cgccgaaggt tccgaggttg ccgtaccgga gcgttccttc
    23821 gtcgagctgc ggcctacccg tgacgtcgtt ctggaaacgc gggttcttac cgctgtgcat
    23881 gcggtagccc acgttcacgc cgacgcggaa gcgcccggtc gaaccgaaac gattctctgc
    23941 gatgagctgg ggccagagcg aaaagccggg atcccagccc atatctcggg gcgccgatcc
    24001 cagaggaatt ccggcctgaa tgacggcgcc aaggccgatc gttcggtcca cccgcgtgaa
    24061 gcggaactta ccgtgcagat tcagggcgcc gatcttctgg gcgttgagat cgccggagtt
    24121 atagagcgcg ctattgggac cgatttgata ggcggtgtca cccgtcatca aaattacggg
    24181 caacgagatg ccgacgacgc cgaagttcgc gagcccgtag ttggctgaaa acgtgccttg
    24241 gaaggagttg ctaatcagag ccggcacgcc ggtgcccgcg ccctgcactg tgcactcgtt
    24301 gctgtcttcc ttgcagggct gaccggcggg ttgaccagga ctctgaccca ctgggatatc
    24361 gtcccggcga gtccggagga ggttgcgacc gtaatcgagc accagcccga aactgatcgc
    24421 attgcggggg atgatgtcgg acccgttgac cgagaagaag cccttcgaat cgaccgcggg
    24481 gcggaacagg tgcgtatcca tgcccgcgcc gttttgttcc gggactcgcc gatccacggg
    24541 cgtctgagcc agtgcttgac gggacgtgcc cagcaggccc aatacgacca gagcgctccc
    24601 gacgtgcgag aggagccgcc cggtcagtgc cgttcgcatc gtgctcgacc tttctttacg
    24661 attccttgcc gagaatcttt acgatcccgt gccgagaaca gagactgcgg atgccgcgtt
    24721 cgctgtgccg cgcagccata tccctccgca cggccgtacc cctccccgcg cccatacgct
    24781 tgagctgttc tcctgagcgg tgtgttcatg ctccgattct tgagctcggc aatgttgcgg
    24841 acgctagcat caggcaaaat cctatgtcaa cgaagcagaa gccggattct gcgcccacaa
    24901 acctgtgcga ctgtatggat gagggcattg gttgtgaggc aggcattggt tgcgaggcag
    24961 gcatcggcgg ccaggcgagc gcgggccgtg cgtggagcac aggtcgccac gcaaacaccg
    25021 cgcagctccg gagtaacgat cgcgggagag acgatcacgg gacacccgaa gcaagtgcac
    25081 gttcagtggg agcgtgggtg gtgcgttcac gaggtgcttc gttgaatgtg cgaacggtca
    25141 gtggggcacg tttcggatca ccagctgcga tggccttctg ccggctgctg tggagaactc
    25201 ttgcctgcgt gtcgctgacc acgctcgcgc acgcgcaatc ggtcacgctc gcgggccgct
    25261 ggtcagccag tgcactccgc accgattgga atgtagggga ttggggttcc gcgtgcgggg
    25321 aacgccccag tggcgcgggt gccgcgagcg ccggagccgg agcgggcagc gtgacgatcc
    25381 gtgtttcggg caaggagctg agtatggcgt ttccgggccg tgccttctcg accgcggagt
    25441 gttgggaaca gctgcccggt atggtccgag agagtcacag cgctggcgcc cgcagttatc
    25501 gaactgtttg caagagccaa gcggctgatc ctcgacgcgc cgtcatcatc acgaccatca
    25561 gcgcgaccga caaccagatt caattcgacg agaccggtca gtaccagttc gtcatccaaa
    25621 accaaaactg caccgcgagt gttcgccgta cgcgcgtgct gacgctcttg caacgcgaag
    25681 gcgatcccgc ccccgcggcg agcccggccg cgagcgctgc caggccggcg cccagtctgc
    25741 cctcgcctac caagcccgcg ccacgttgtc agacgacagg cttacccgag cgcatggagg
    25801 tgcggccttc acacaaactc gcacgccccg gcgagagctt cgcgtttcga agcgcggtgc
    25861 tcgatgcggc cggctgcagt ttgccaatca tgcccacctt ccgaatcgtc aagggcggtg
    25921 cgctcggcga gttgagcgga gcgggtaagc tcaagatttt cgaccaggca gaagagggcg
    25981 agctcacact cgaggcgacg gtcggtgacc gatcggttca ggtgcgcgtc caagtcgtgt
    26041 cgcaggaacg ctacgacgcg ctgctgaaac aaggtgactt caacgagcag ggtgagtctg
    26101 cggaggccgc gagcacgcgc atcgcatcga gttcgattgg ggctcgatcg ggagtcgccc
    26161 gcgatgaagc gcggggcagg cgcatggcct tcttggccat cgtcggcgcg ctctcgctag
    26221 tgctcggctg tgcaggtcta ttcatcctgg tgcgcacgcg gcgcgctcga cccaagcgct
    26281 ctatctctcg cgaggagctc gcatcagcca ttacgggtcc cgatcacttc ccagacgcgc
    26341 agctcgcgag cttgcgcccg acgagccgcg tttgcccaac atgccgggaa gaatacccgc
    26401 cccacgccga gttctgtgcg aacgacggta accgcctcct tccggtggtg gccggagcgg
    26461 gagtgagcgc aaacagcatc gtggctccaa ccggcggcgt ttgccccatc tgtggccagg
    26521 gttacgaccc tggtgtgaat gtttgcccga agcaccaaga acccctggtc cccgtcacgg
    26581 tttgggcgga gcgccggcgc cgcgaaaata cgctcattcg caagatttgc ccggtttgtg
    26641 gcacgcagtt ctcgggtgac agccagttct gtgggaaatg cggcgccgcg ttagttccgg
    26701 tcaactgagc gcgttgcctc ccgcgaacgc cgtgcggcat gacccgaggc ctcgagcgcg
    26761 tgcagcacta atcggagacg aactcaacgg caaaccctcg agcatccgga tttcgcctcg
    26821 ggattttgct aaggtccagg gccgttcgtg gttagaactt tcgctgcaaa cggtaatgcc
    26881 cgacaccctg accgccactt gcacgtggcc agtgtggttc cccccgtcct agaggaacga
    26941 tgaagattac gtgtgactcc tgtggagcca agtacacgat agccgacgac aaggtacgcg
    27001 gccgtaaggt gaagatccgt tgcaagggct gcagtgcgcc gatcgtcgtc gatggccagc
    27061 agccagaggg cggaatgcgc atcgaagggg gcagcaccga cgcaagcggt acgccgagcg
    27121 tttcttccgt gccgcccggc ggagcgtggt ccgtcaactt gagcgagacc gatcagcaga
    27181 cgctgaccac cgagcaaatc gttcaaggct gggctggcgg acaagtcacg gccgacgcgt
    27241 tcgtgtggcg gcaaggcatg gcggattggt tgccaatcct ggagtgcgcc gagctcgcgc
    27301 cgttgctgaa tggtacggcg gccgctcccg ctgccgcgct cgctcccgcg ccggcaaagt
    27361 cggatagctt ggtgcccgcg ggtggacgcg cggcggtgag cgcacgcgta gtcggcggac
    27421 gtgctcagaa cacgatggat ttgtttgcgg gcgtcgacaa agccggcgcc gacggcgtgc
    27481 acgacgaaag tgaagacgtg gtgatgcccg gggcaggccc ggccctgccc aatcgaccgc
    27541 agaccgccga ggaggcgcgt cccacgggcg cgcgcaacga gaactcggtg ttgttctcgc
    27601 tggacgcgct gaaggcaggc gttacgacgg gtggcaagcc cgagccccag aagcccagcc
    27661 gcgcgcccca gcgcggtgtg gttgccgaag atccattcaa cctgagcggt ggcggcgtcg
    27721 ccgcgctgca tggcgggccc atgttcagcc tggccgacaa tcaggcgctc ctcaccgcac
    27781 ctccgccgcc cgagccaccc aaggtcgtgc agtcgttcgc caccggcgcg ccaactccac
    27841 aggccaagag ctcgagcaag ggcgggctcg tgattggtgc catcgtgctg ttgctcgcgg
    27901 gcgcaggcgg cgcggtgttg ttgatgggtg gcaagaaaga agtcgccgag aacaagacca
    27961 ccagcagcga gattcagaag aaggacgagt cagccgcggc ttccgagacc aaagaagagc
    28021 ccaagaagga agaagccaaa gctgaagagc cgaaggccac cgagagcgag aagaagccgg
    28081 aagaaaccgc gtccaaagaa gaaaaacctg ctgatggcaa ggcggccgag accaagccgg
    28141 aagaaggcac gaaggcggta gcgagcacga aggccggcac gagcggcagc accgcgtcca
    28201 ccaagcccaa gaaggaagag cccaaagagg aggccaagcc ggccggggga gaccagccct
    28261 tcagtaagtc ggcggcgatc gcatcgctcg gcgcggccgc gaaccaggcg ggcagttgca
    28321 agaagctcgg cggccccacg ggttcgggca aggtcacggt caccttcgcg agcagcggga
    28381 aagtcacgag cgccaacgtc agcggcccac ccttcgcagg cacgtccgtt ggcggttgcg
    28441 tcgcatcgat cttccgcaag gctcacgtac cgcccttcag cggtagctcg gtgaccgtgt
    28501 cgaagggctt cacgatcaac tgacacggcg gcgggtgcac ggccgcgacc ccgcgaacta
    28561 cgccctggca cgcacgtacc aaaccgtgcg gtcagggcgt tcgtaatttc ggtgcgcgtc
    28621 ctgcgcgacg cgcaccgcac gtgcagcgcg cccgtccccc cggcgaaagt cacgcccgga
    28681 gctgcttcct tcgacgacga atgccaagct ctgggccgtg actcgtgtcg catgcggcaa
    28741 gcccagggaa acgtacggat gtcctcgctg atcgagctgc agaaggtccg caaatcttat
    28801 cgcagcgggc aagaggtgct gcccatccta cgtgacgtcg atctgagcat tggcgaaggc
    28861 gagttcgtat cgattatggg ctcttctggt tccgggaagt cgaccttact caacattctc
    28921 gggatcctcg atcgctacga ctctggaacg tactacctcg acggacgcct gattcgagat
    28981 ctctccgaga ccgaagcggc gcgctaccgc aatcgcttca tcggcttcgt gttccaggcg
    29041 ttcaacctac tgccgttcaa gaccgccgcc gagaacgtcg cgctgccgct ctactatcag
    29101 ggcgtctcgc gcaagaaacg cctcgagctt tcgcgcgagt atttggagcg cgtggggctg
    29161 gcggcccggg cggagcacct gccaaaccag ctctcgggcg gcgagaagca acgggtcgcc
    29221 gtggcgcggg cgttggtgac ccgtccgaag ctactgctcg cagacgagcc aacgggagcg
    29281 ctcgacaccc gcacgtctta ccagctcatg gagctgttcc aggcgctgaa cgagtccggg
    29341 atcacgctgg tgatcgtcac gcacgagcac gacatcgccg aacgcacgcg tcgcgtgatt
    29401 cgtttgcgag atgggcgcgt ggaagattga ggggggatgc tgctcgaagg cctactcgaa
    29461 atcgcgcaga cgctctggca gcacaaactg cgcacctttc tgacggcgct gagcgtggcc
    29521 tggggcacgt tcatgttgag tttgctgctc gggctcggac aagggctacg taccggcgcg
    29581 cagtgggagt ttcgagacaa cgcggtcaat tcgctctggt tcttcaactc gaccaccagc
    29641 ctctcgtatc gcggtcgcgg cccgggccga tcggtctact tcaacaacga agacctggac
    29701 gtgctcggtc gcaggctgcc ggaggtgcag taccgttcgg gccgctatta cttctggggg
    29761 gagttcttcg tgagctacgg cgcgaagcac gcctcgttcg atattcgcgg agtgcacccc
    29821 gagcatcgct acatcgcgcg cacgcagatg ctccaggggc gcttcgtcaa cgacatcgat
    29881 ttgcgcgaac accgtaaggt cgcggtgatc ggctccaaag taagggagag cttgttcgag
    29941 aaggaagacc cgattggaaa gcagatccaa atccgtggcc tcacttatca ggtcgtgggc
    30001 gagttcgccg acgagggcgg cgaggccgag ctacggcgaa tctacgtacc gctgaccacg
    30061 acgcagctcg tttacaatca gcccaacatc atccacaacc tgatcctcac cctaaagagc
    30121 gacgatctgg ccgagagcag cagagtggcc cagcaggcac gcgccatctt gggggaccgg
    30181 cacgacgtgg cgcccgagga ccgacgcgcg atccgtgtgc aaaacaacct cgagcagttc
    30241 gccaagacca tgaacatctt cgtctggctc ggggtgttcg tctggatcgt gggcgcgggc
    30301 acgctgctcg cgggcgtggt gtccgttagt aacatcatgc tgatctcggt gcgcgagcgc
    30361 accaaggaga ttggaattcg caaggcgctg ggcgcaactc cacgctcgat cgtcggtcta
    30421 atcgtgacgg aagcgatgtt gctcacttcg gtatccgggt acttgggcct attcgggggc
    30481 gtgtttctga tcgaggccat cgccgaccgc acgcaaggcg tgccgttcat gcgtcaaccc
    30541 cacgtggatc tacgctcggt actgattggt gcggggttgt tggtagtggc gggggcgctg
    30601 tcggggctct ttcctgccct caaagcagcg cgcgtggatc cgattgtggc tctccgcgag
    30661 ggggacacgt gaacctcgac tcgtggcaag agctgctgca cacgctctcg gccaacaagc
    30721 tgcgcgcatt cctcacggcc tgcggggtat tctggggcat cttcatgttg gtgttcatgc
    30781 tcggcatggg caccggactc gagcgcggcg tgcgtcgcag cctcggcaac atgacgagcc
    30841 gcgcggtata cgtttggagc caacgcactt cgctgcctta tcgagggctg cagcctggtc
    30901 gttatctcaa gttccacaac gcggacgccg aggcgctacg gcgcgtgccc ggcgtcgtgt
    30961 atgtcgcccc gcgcttgcaa ctcggcgcct ggcgtgctgg cacgaacgcc gtttacaagg
    31021 ggcgcaccgg caacttcgtg gtgctcggtg attcacccga gttctttcgc gtggaaccga
    31081 gcacgctgaa acgcggccgc gtattcaatc cccgggacca agccgaggtc cgcaaagtgg
    31141 ccgtgatcgg cgaccaagcg cgccacgtgc tgttcggtga ggaggatccg atcggcaagt
    31201 acatctccgt acgcggcgtg tacctgatga ttattggcga gatatcgtcg gagaaatccg
    31261 gcgacgacgg cgagcgcatc cgctcgtcct tgttcgtgcc gctcagcacg ttccaagccg
    31321 ccttcaacca gcgggaccga atcggctggt tcacgctgac cgcgcgcgag gacgtggatc
    31381 ccgaacagct cgagcagcgc gtgaaggcca ccttgcgggg tcgccacgga gttcatccgg
    31441 aagatcgaga tgcgctcggc tcgttcaact tggcggccca aatcaacaag gtcaatgggc
    31501 tgtttcgcgg gattcagagc ttcgtctggt tcgtcggcac gatgacgctc ttggcgggcg
    31561 tactcggggt cggcaacatc ctgctgataa ccgtcaaaga acgaactcga gagttcggcg
    31621 tacgtaaggc gctgggggcc accccgtcgt cggtcgtcaa catggtgctc cgcgaagcgt
    31681 tgctgctgac ctcgatggcg ggctataccg ggctcgttgc ttcggtgggc gggctcgagc
    31741 tgatggcgcg cgtagtggcc aagctccaga atgcgccgat cgaacggccc gaggtagacc
    31801 tgcgcgtggc tctgattgca acgctcgtgc taatcatcgc gggcacgctc gccggtctcg
    31861 ttcccgccgt gcacgccgct cgaattcgcc ccgtggaagc cttgaggtcc gaatgattcg
    31921 ccgtttgctc ccgctgctgt tcgcggccct ggtgctcgtg ggatttggct ggactctggt
    31981 gttcttgtac aagaaatccg aggccaagcc ggccgcgttc cgcagcgtat cgcccgagcg
    32041 gcgcacgatc gtgaagaaga cggtggcgcc aggcgcgatc gttccgcgcc gggaagtgac
    32101 gatcaagccc cgcgtgtccg gcgtgatcga aaagctctac gtccagccgg gacagtacat
    32161 caaagagaaa gctctcgtcg ccaagattca gatcatcccg aacatggtta gtctcaacaa
    32221 cgccgaggcc aacctgcgct ccgcgcaaat caacttggac accgcgcagc gcgagttcga
    32281 gcgcttcgag aagctcaaga cccaaggcct gacgaccgag accgagttcg tgcagcgcga
    32341 gctcgcgttc aagctgcgcc agggcgaacg agacgccgcc gagaataact tgcagctcgt
    32401 gcgcgtcggg gcgtccaagc aatccggcaa agtctcgaac gtggtcctcg ccacgctgga
    32461 cggcatggtg ctcgaggtgc ccgtcaagga aggcggcagc gtgatcgagg ccaacaactt
    32521 caacgaggga accacgatcg cggcgctcgc agatatgcgt gacatgatct tccaaggccg
    32581 agtggatgag tccgacgtag gtcgggtcaa agccggtatg cccgtcacga tcaacgtcgg
    32641 ggccctgggc gaggagcgat tttccgggac cctcgagtac attgccccca agggcgtcga
    32701 gaaagagggc accatccagt tcgaggtacg cgccgcggtg cagctcaaac aagacacgtt
    32761 cctgcgcgcc aactacagcg ccaatgccga tatcatcctg gatcgaaagg aaaacgtgct
    32821 ctcgattgcc gaagccgacg taactttcga gtcggggcag accttcgtcg aagtcgagga
    32881 cggcccccag cacttcgttc gccgcaaagt cgagctcgga ctatccgacg gcatttacac
    32941 ggaagtgaag agcgggttaa ccgagagcgc gcggatcaag aaacacgagt agacccgagc
    33001 cgacggagcg cggtatcgaa gagcgaaggg ggaggcgtcg acgtgaagcg gagcgaaaac
    33061 cactggtata tcctggaaat cctcgcgaag agcatcgcgc gctcgtatcg actgaacagg
    33121 gttcgaatag gattctgggc gctcggtatc gtgctgtgtc tgacggcgat tcagacggat
    33181 gcaatgcgca ccctattgag ttccgcggac acctcgcgag cgttgtccct ggccgtgccg
    33241 gcggcgctgc tcgttgtttt tctaggtatc gtccaggtgc gacaaagggc acaaaggcag
    33301 gcctactggt ccgctttgcg tgaaccc
//
LOCUS       DASTKU010000146         4347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1588719, whole genome shotgun sequence.
ACCESSION   DASTKU010000146 DASTKU010000000
VERSION     DASTKU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588719"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VFQ61_05265"
     CDS             <1..1403
                     /locus_tag="VFQ61_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET9953886.1"
                     /translation="KDPRSKASGVLRHMNPLQELVRRLGDVITTDPDVLPGYCRDAAS
                     WATAGAPLALARPRATEHVQTLARWATENRIPLVARGAGTGVVGGANAVNGCVVVSFE
                     RMNEILELNDSAMYAVVQPGVLNVELKKAAEAFDLWYAPDPASYEISTLGGNVATNAG
                     GLCCVKYGVTGDYVLALEGVLASGERFRTGVPCRKDVAGYDLKRLLVGSEGSLALITE
                     ITLRLRRRPKRPGTLVALFSSLEAAGRAVSRVVRAVEPALLELMDRTTLRAVEGYAQL
                     GIDHDAAAMLIGQSDTGNPAELELMREACESTDPRLVYITDDEHEGQLLLRARRLAFP
                     ALERLGNVVSDDISVPLPRLPEMLRRIEVRAREQEVLVGTVAHAGDGNIHPLIVRSPG
                     SGAEREQALFERLMLDAVELGGTVSGEHGIGSIKADFLNLQLDETALSLHYAVKQAFD
                     PLGILNPGKLILPSRR"
     gene            complement(1464..2759)
                     /locus_tag="VFQ61_05270"
     CDS             complement(1464..2759)
                     /locus_tag="VFQ61_05270"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9953887.1"
                     /translation="MRRIEHGRKLGLLMFAGAAFGISTSCSHDVTSGEQLDALGTDSY
                     EIVGGEVAEASPWAVQVFDVRGGTGDFFQCTGTLVAPRWVLTAQHCVADEGETSAVRV
                     GSNTYREGRRITVDAVEVPSAAADIVLLHLSEDAEGPFVQLAQSTSVAIGRDALGYGW
                     GSEGTPLVMAPSLKKATLHIDSWYSSEELLATGVTGSGWNGDSGGPWFVDGRQVAVFS
                     AVSGQDGTNLHARQLGVDVARHRAWIDATVASGPTAHPTGQVVLYDLSNFSGIAQSFG
                     VGRHDLPNSTVMGNDRASAIRVPAGLRVTAFYNGAFGGEARVYTSDTNLIAAGFNDVI
                     SSIIVSPASDPQLDVVTFYENASFAGERVIYGLGSGHFLSSPWDQRASSVRVPSGFKL
                     TLYDGLWRYSAESRVVTTDANLASQNFDNKTRSFLVERL"
     gene            complement(2871..3329)
                     /locus_tag="VFQ61_05275"
     CDS             complement(2871..3329)
                     /locus_tag="VFQ61_05275"
                     /inference="COORDINATES: protein motif:HMM:NF040285.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5980 family protein"
                     /protein_id="HET9953888.1"
                     /translation="MKSIHRFNKLSITFVGALAAAVVAGSPAAAKSNAVTASAWTLND
                     YNQRVCYAPGTGFTYFLVSVTGTWSNPIQWSLSSVPSGWSDYGPGTIPSGSAGPGAVQ
                     ALAALNISISAAEGDYTIPLTASDGLQTQSVPLLVRIRKGCSGGWPSGMN"
     gene            complement(4014..>4347)
                     /locus_tag="VFQ61_05280"
     CDS             complement(4014..>4347)
                     /locus_tag="VFQ61_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953889.1"
                     /translation="SRLYRVDPNTGGRTQLGGVDWAGPTLLASENDRLFAVQGERLWS
                     IDPATGAFSRLGNDTWRSATSMSALSGRLLLIDNGWLHSIEPSTGNYEGLNQQAWLGS
                     TRMTIIPR"
BASE COUNT          834 a         1290 c         1407 g          816 t
ORIGIN      
        1 ataaagaccc tcgttcaaaa gcatctggag ttttacggca catgaacccg ttgcaggagc
       61 tcgtacgtcg tttaggggat gtcatcacta cggatccgga cgtgctgccg ggctattgcc
      121 gcgacgccgc gagttgggcg accgccgggg cgccgctcgc gctcgcgcgc ccgagagcga
      181 cggagcacgt tcaaacgttg gcgcgttggg caaccgaaaa ccgaatccct ctggtcgccc
      241 ggggtgccgg cacgggcgtc gtaggcgggg caaatgccgt aaacggatgc gtggtcgtga
      301 gcttcgaacg catgaacgag atcctcgagc tgaacgactc cgccatgtac gcggtcgtgc
      361 agcctggcgt gctcaacgtg gagctgaaga aggccgccga ggcattcgat ctttggtacg
      421 cgcccgaccc ggcaagctac gagatctcga cgctcggcgg taatgtcgcg accaacgccg
      481 gtggtctatg ttgcgtcaag tatggtgtga cgggggacta cgtgctcgct ctcgagggtg
      541 tactagcgag cggcgagcga tttcgaacgg gcgtgccctg tcgcaaggac gtcgccggct
      601 acgatctcaa gcgtttgctc gtaggatccg agggaagcct ggcgctcatc accgaaatca
      661 cccttcggtt gcggcgaaga ccgaaacgac cgggcacttt ggttgcgctc ttcagcagct
      721 tggaggctgc aggtcgcgcc gtgtcgcgcg tcgttcgtgc tgtggaaccc gcgctgctgg
      781 agctaatgga tcgtacgacc ctccgcgcag tggagggcta cgcacaattg ggaatcgacc
      841 acgacgcggc ggcgatgctc atcggacaaa gtgacaccgg aaatccggcc gagctcgagt
      901 tgatgcgtga agcgtgcgag agcacggatc ctcgcctcgt ctacattaca gatgacgagc
      961 atgaggggca gctgctcttg agagcgcgtc gactggcctt tcccgcgctc gagcggctcg
     1021 gcaacgtggt gagcgacgat atctcggtac cgctccctcg tttgccagaa atgttgcggc
     1081 gcatcgaggt tagggctcgt gagcaggagg ttctagtcgg aaccgtggcc cacgctggag
     1141 acggcaatat ccatccactg atcgtccgca gtccgggcag cggtgctgaa cgggaacagg
     1201 cgctgttcga acggttgatg ctagacgccg tagagcttgg cggaaccgtg agtggagagc
     1261 acggtattgg ttcgatcaaa gcggacttct tgaatctcca gctggatgag accgcgctca
     1321 gcttgcacta cgcagtcaag caggctttcg atccgctcgg gatactgaat ccgggaaaac
     1381 tgattttgcc gtcgcgaagg tgatcgcgat cgccgttgaa agcaatcgct cacacggcgt
     1441 gacctcttgt cgacgagttg agactacaag cgttcgacga ggaagctccg ggtcttgttg
     1501 tcgaagtttt gggaagcgag gttcgcgtca gtcgtcacga cgcgagattc ggcggagtag
     1561 cgccagagtc cgtcgtagag tgtcagcttg aagccggagg gaacgcggac ggagctggcc
     1621 cgctgatccc aggggctgct gaggaagtgg ccggagccga ggccgtagat gacgcgctcg
     1681 cccgcgaagc tcgcgttctc gtagaacgtg accacgtcca gctgcggatc cgaggccgga
     1741 gagacgatga tgctcgagat cacatcgttg aagcccgcgg cgatgagatt cgtgtcggac
     1801 gtgtagacgc gcgcctctcc cccgaaggcg ccgttgtaga aagccgtgac ccggagcccg
     1861 gcaggaactc ggatcgcgct ggcgcggtcg ttccccatga cggtcgagtt cgggaggtcg
     1921 tggcgcccca ccccgaagct ctgcgcgatg ccagagaagt tcgagagatc atagagcacc
     1981 acttggccag tggggtgcgc ggtggggccg ctggcgaccg tggcatcgat ccaagcgcga
     2041 tggcgcgcga catcgactcc gagctgccgc gcgtgaaggt tcgtgccgtc ctgcccgctc
     2101 acggccgaaa acacggcgac ctgacgccca tcgacgaacc acggaccccc cgagtcaccg
     2161 ttccagcccg agcctgtcac gcccgtggcc aggagctcct cgctggagta ccagctgtcg
     2221 atgtggagag tggccttctt gagcgatggc gccatcacca gcggcgtgcc ctcgctcccc
     2281 cagccgtagc cgagggcgtc ccggccgatc gccactgacg tcgactgggc gagctggacg
     2341 aagggtccct cggcgtcctc actcaggtgg agcaaaacga tgtcggctgc ggccgagggc
     2401 acctcgaccg cgtcgaccgt gatgcgtcgg ccctctcggt atgtgttgct acccacacgc
     2461 acggctgagg tctcgccttc gtccgcgacg cagtgctgcg cggtgagcac ccagcgcggc
     2521 gcgacgagcg tgcccgtgca ctggaagaag tctccggtgc ccccgcgcac atcgaagacc
     2581 tggacggccc aggggctcgc ctcggcgact tcgccgccga cgatctcgta agagtcagtc
     2641 cccaatgcat cgagctgctc gcccgacgtg acatcgtgcg agcaactcgt cgaaatgccg
     2701 aaggcggcac cggcgaacat cagcagacca agcttacgac catgttcaat cctcctcatt
     2761 gcgactccat attacgactc tgcttagaac tgttcagacg atcatcggtg ccgactgggc
     2821 tccttcacca ggagctgacg ctctttagta tcggagtgac gggcgagacg tcagttcatt
     2881 ccggagggcc atccgccgga gcagcccttg cggatacgaa ccaagagcgg cacgctctgg
     2941 gtctgcaagc cgtccgatgc ggtgagtggg atcgtgtaat ctccctccgc cgccgatatc
     3001 gagatattca gcgcggctag cgcttgaacg gcacctgggc ctgccgaccc agaggggatt
     3061 gtccccggcc cgtagtcgct ccaacccgat ggcacgctgg aaagcgacca ttgaatgggg
     3121 ttggaccaag ttcccgtgac cgagacgagg aaataggtaa atccggtgcc cggagcatag
     3181 cagacgcgtt ggttgtaatc gttgagtgtc caagccgatg ccgtgaccgc attggatttt
     3241 gccgcagcgg gggagccggc gaccactgcc gcagcgagtg ccccgacaaa cgtaatcgac
     3301 agcttgttga aacggtggat tgatttcatg aggactcctt ggttcacgag tgcgctgatt
     3361 gacgagaaac tctcggtcta cgtctgaact ggttgccctg ttgtgtcggc ctgatggtat
     3421 aggcctgctt gtgtccacca accccgtaaa tcggatcttc cgtatcggaa tttctaacag
     3481 aagacagact ggcaatcaaa cgattccaac tcgaaagttc gaattggcga atggccgaaa
     3541 ttggcagtta tgtgtgaact gccgcgtgac attgcgtcac agaggcatcc ttcacagcgg
     3601 caagcgtcac ggccataagc gttgcgagcg tgaggcatgc gtatgaatcg tacgcatgcc
     3661 agggagtgaa cctaagtaac gctgtcgaag agctgtaggg ctacttgaag tcaagcgagc
     3721 caagttgcgc accgatcaag gtttagtccg acccgggggg cctcgctgcc ggtccgatgt
     3781 tgggcaaatg gcgccattcc agccctcgaa agcctcaagc ggatcttgcc gcgccgggct
     3841 ttccatgcgc gatggtctgg gctgcactcc tgagttcgag acgggatcgc catgagcatg
     3901 tttcaaccgg cgaccgcatc agcacccgct gatgggcgag ctcacccatc ggtacccgct
     3961 gggtaggtca cgagaccgat gggcaaggtt atggcactcc gctctttgaa tgctcagcgg
     4021 gggataatcg tcattcgtgt cgagcccaac caggcctgtt gattgagccc ttcgtagttg
     4081 cccgtggagg gttcgatcga gtgcagccag ccgttatcga tgaggagcag ccggccgctg
     4141 agcgcgctca tcgaggtggc gctccgccat gtgtcattgc ctaggcgtga gaaggcgccc
     4201 gtcgcgggat cgatgctcca caatctctcg ccttgcacgg cgaacagcct gtcgttctcc
     4261 gatgcgagca gtgtggggcc cgcccagtcg actccgccga gctgcgtgcg gccgccggtg
     4321 ttcggatcga cgcggtacaa ccgcgat
//
LOCUS       DASTKU010000147         8818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1365525, whole genome shotgun sequence.
ACCESSION   DASTKU010000147 DASTKU010000000
VERSION     DASTKU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365525"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VFQ61_05285"
     CDS             <1..644
                     /locus_tag="VFQ61_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET9953890.1"
                     /translation="QEAYLRGELERRQAEFANYPERQYLQDIVSDLQDDLLEEKSADR
                     AIRRVWASLWSERAYHYREYYGIDHRRVYMGVAVHPTFVGERLESVVFTNLEPDADMP
                     LYRVISQVGEVGVAEPSDPSATPEILSFRRDAAGEPHDITLVAPSSLAPGGARLWDDP
                     EISELARALFAVQEHFAKVVYPQLTPLSLDVEVDIARDGRIVIKQARPYTPRL"
     gene            complement(671..877)
                     /locus_tag="VFQ61_05290"
     CDS             complement(671..877)
                     /locus_tag="VFQ61_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953891.1"
                     /translation="MTTSHSGKLERRRCSGRTPGTYANPHHHRITDRPDTLDYEFMAQ
                     VANMVRVAVSNPTPADIERKLRFL"
     gene            984..2384
                     /locus_tag="VFQ61_05295"
     CDS             984..2384
                     /locus_tag="VFQ61_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953892.1"
                     /translation="MFSWSLFTWTWFRSRSAQLGSCLALLVVLVVTGSLHLYAIEPST
                     TGKQLDDEGAYLPPALQAKRRSEYDWLPGRLPYSHRPPLTVAVYSNIIRVADARSLPE
                     ALTAIRQFQTFVLLGILVLSYIQGWLLGLGPGWSLFAPCLIAMAPRFGIYVHLAMSEL
                     FCALLGAAGVSALLAFFRDFRARYLALAGLVFGISLLVRASFKPFVAVLLPFLVAELV
                     WRERAWRHLRAPRVIARVLLPVLALTVGVLIAVGPQLATNQARGNGLHISNNTWRNIE
                     FGFRSRVPGSDDASGISWAHMRAEWLRRAKTDEQRELAAKRSTLEFLEAMPRSEIAAR
                     QLTKMAYRLVVDERTQFEAARGNERFRPTADWIWHFEGAAVGLWIFTLFTGMLGFVVS
                     FRRGPAWRVLFLFWAALLSGFVLVPTSQRFVVVAVPVLCLGSAIGVQYVTKRLAEVGT
                     GWRGRLRSYRPRPSGY"
     gene            complement(2391..4040)
                     /locus_tag="VFQ61_05300"
     CDS             complement(2391..4040)
                     /locus_tag="VFQ61_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PE PGRS family protein"
                     /protein_id="HET9953893.1"
                     /translation="MRSSTLKSLGHLSVVMTLMQLGCSSGSKEPGANGGSNSAGTTTS
                     GGISATGGSSATAGSSATAGTNAMGGTAMGGTAMGGNTAMGGNTATGGNTAMGGITAT
                     GGITAKGGNTATGGTSAAAGGTNPTGGSSPGGAGSGGTNTAGTTGVGGGTPGTVNWGK
                     EEDPSAGCAVNATLPAYADLKADGKLPDPFTKLDGTRIKDKSEWACRRAEIVKQHFKY
                     VFGEKPIPAKGSVSGTVSTSKISVKVTEGSKSASFDVTVNMNGATAPAPAIIIYDGGL
                     GSSLPIPSGVAKITMSAIEAQGGTGNKSGPFFTFYGANHPAGYMVAQAWQVSRIIDLL
                     EQNPGTIDPYHIGVTGCSRNGKGAFAAGVFDNRIALTIPCESGIGGTVGLRLKEQLDP
                     GANDTAEWPYHAISYVRWLSEVALGPFATANNAAGDNTDRLPVDMHEAMALIAPRALY
                     IVDNPGISNLDPKSAYVTAMAGKAIFTALGVADNFAYQGASGSHCQWRTQYDASLTAM
                     IDRFLKGKDTTKTGNFATDLGSKPNTAQYYTWDAAELPGKL"
     gene            complement(4401..4715)
                     /locus_tag="VFQ61_05305"
     CDS             complement(4401..4715)
                     /locus_tag="VFQ61_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953894.1"
                     /translation="MRAVQDAVDGLENAYRALCTCQEVQSPDGSCEFEGYDAVAVREA
                     LEHHAERGDGFTECIRDIVQPLSACLSTEHGCEYGACPHVAALLDDNTVLSELGESCA
                     RK"
     gene            5341..6423
                     /locus_tag="VFQ61_05310"
     CDS             5341..6423
                     /locus_tag="VFQ61_05310"
                     /inference="COORDINATES: protein motif:HMM:NF045039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953895.1"
                     /translation="MGGAAGPGSGGTPTTGGNGGGGFGTVDAKGGASGSVGATGGASG
                     AAMGGSGNAGGASAGMSSGGQAAAGAGTGGFAAGAAGAGGSGAGGNSAGGNGSGGSSA
                     GATNTGGGSGSGGSSVGAGGSGDSGGSSGGASGSAILTEGFEGTALPSGWDNFIGYVK
                     NGMNPQGEFKIVIDSSKKHSGSSSVHFHGGSTPAMLTRPLPANTKKLYVRVWVWLSRS
                     LGNNPDGNHETLLGIRKNSGTADNEVRFGEIKGAIGVNEVPTDALSLKPELWNKGPTI
                     AKEKWVCLEAAFLGDKATHEFYAWADGMEVHKIIGASQWHAPVAADFLTGKFNEFIIG
                     WQSFSNFTADVWADDLVLSTERVGCN"
     gene            complement(6461..7213)
                     /locus_tag="VFQ61_05315"
     CDS             complement(6461..7213)
                     /locus_tag="VFQ61_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9953896.1"
                     /translation="MVPPPDDTEVQRTALRERILEAARRLILEGGKDAATTRAVAAAA
                     SVQAPTIYRLFGDKEGLLDATAQFALEKYVAAKRARAPHPDPIQDLRSGWDEHVAFGL
                     SQPSLFALMNGDQRFQTTPAARSGEEVLRKKLRRLAEQGLLRVSEDRALALLQAVGVG
                     VIKRLLEQPANERDLGLADLARESVIHAIAGKRNPPVATGAVGAAVALKAGLDSVNVL
                     SAGERLLLEELLERIASADASAPRQVRKNRNR"
     gene            7423..8280
                     /locus_tag="VFQ61_05320"
     CDS             7423..8280
                     /locus_tag="VFQ61_05320"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9953897.1"
                     /translation="MIVITGATGQLGGAIVRSLLQRAPGARIAASVRNPEQAGALAQL
                     GVEVRRGDFSRPETLVAAFSGATQVLVVSSNAGAFGADPVAQHRAAIDAAKQAGAQRI
                     LYTSHVAASAWSSFSPMHTHAATERILAESGVRWTSLRNGFYAASALVFIGDGLEKGR
                     IAVPDDGKVVWTTHADLADAAAAVVLDEGCDGPIPLTGGEGLDMADIARIASEVLGRN
                     IERESISDAALEERLKALGVPPPRADILLGFYRASRAGEFARVDGTLEKLIGRKPQSM
                     RSVLERLPA"
     gene            8364..>8818
                     /locus_tag="VFQ61_05325"
     CDS             8364..>8818
                     /locus_tag="VFQ61_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953898.1"
                     /translation="MEKIPTIFERGERFKVINQPRTSCDWVFAGQGRATEKLDGMNCR
                     LTIRTGQVVRVEKRRNPSKLQKDQGIVDGWYVDADEQSNEDKWIFEAVRGTEVREWPD
                     GEHSVEALGPSIQGNVLGLAKHICVPFNLRIPVYEDVPRTYEELKSFLAT"
BASE COUNT         1540 a         2695 c         2849 g         1734 t
ORIGIN      
        1 agcaggaggc ctacttgcgt ggtgagctcg agcgacgtca ggcggagttc gcgaactacc
       61 ccgagcgcca gtacctccaa gacattgttt ccgatctgca agacgatctg ctcgaggaga
      121 agagcgccga ccgcgcgatt cggcgcgtgt gggcgagctt gtggagcgaa cgagcctacc
      181 actatcgtga atactacggc atcgaccatc ggcgggtgta catgggggtc gcggtgcacc
      241 cgacgttcgt cggagaacgg ctcgagtcag tggtgttcac caatctcgag ccggacgccg
      301 acatgccgct ctatcgtgtc atcagtcaag tgggggaggt gggtgtggcc gagcccagcg
      361 acccgagtgc tactcccgaa atcttgagct ttcgccgaga cgccgcgggc gagccgcatg
      421 acattacgct cgttgcgcct tcttcactgg ccccgggtgg cgcgcgcttg tgggatgacc
      481 cggagatttc cgagctcgcg cgagctctct tcgcggtaca agagcacttc gcgaaggtgg
      541 tgtatcccca gctgacgccg ttgtccctcg acgtcgaagt cgatatcgcg agggatggac
      601 gtatcgtcat caagcaagcg cgcccctaca ctccgcgttt gtgagctgcg aacgccggtg
      661 gcgacctggt ctacaagaag cggagcttgc gctcgatgtc agccggcgtc ggattcgaca
      721 ccgcaacccg aaccatgttt gcgacctgcg ccatgaactc atagtcgagc gtgtctggtc
      781 gatcggtgat gcgatggtga tggggatttg cgtaagttcc cggtgtccgt ccagagcaac
      841 gccggcgctc cagcttgcca gaatgggaag tggtcactgc gcgtgacggc actgactcag
      901 ttggcccacc ggttgattgt gaccaccttg agaccagtga tccttcaact cggacgcagc
      961 cgtggtaggc agccttctgc cggatgttct cctggagctt gttcacctgg acctggttcc
     1021 gctctcgaag cgctcagctg ggctcgtgcc ttgcgctcct cgtggtgctt gtggtgacgg
     1081 gctccctgca cctgtacgcg atcgagccct cgacgactgg caagcagctc gacgatgaag
     1141 gcgcctacct gccgcccgca ctgcaggcca agcggcggag cgagtacgat tggctgccag
     1201 ggcggcttcc gtactcgcat cgcccgccgc tcaccgtcgc cgtttactcg aacatcattc
     1261 gcgtcgccga tgcccgatca cttcccgaag cgctcaccgc gattcggcag tttcaaacat
     1321 tcgtcttgct cggcatcctg gtgcttagct acatccaggg ttggcttctc gggcttgggc
     1381 caggttggag tctattcgca ccctgtttga tcgcgatggc tccgcgcttc ggcatctacg
     1441 tgcacttggc gatgtccgag ctgttttgtg cgttgctcgg cgcggctggc gttagcgcgc
     1501 tgctcgcgtt cttccgcgac tttcgcgctc gatacctggc gctcgcgggt ctggtgtttg
     1561 ggatttcgct gctcgtgcgg gctagtttca agcctttcgt ggcggtgctc ctcccttttt
     1621 tggtggcgga gctcgtgtgg cgcgagcgcg cgtggcgaca cctgcgggcg ccgcgggtca
     1681 tcgctcgggt gctgctcccc gtcctggcgc tgacggtggg cgtgctaatc gccgtgggtc
     1741 cacaactcgc gacgaaccaa gctcggggga atggtctcca catctccaac aacacttggc
     1801 gcaatatcga gttcggtttt cgctcgagag ttcccggctc cgacgacgca agcggcatca
     1861 gctgggcgca tatgcgtgca gaatggctac gccgagccaa gaccgacgag cagcgagagc
     1921 tcgcggccaa acggagtaca ctcgagttcc tcgaggccat gccccgttcc gagatcgcgg
     1981 cacgccagct gaccaagatg gcctatcggc tcgtcgtgga tgaacgcact caattcgagg
     2041 cggctcgggg taacgaacgc ttccgcccaa ccgccgactg gatttggcat ttcgagggcg
     2101 cggcggttgg gctgtggatc ttcacccttt ttacggggat gctggggttc gtggtgagtt
     2161 ttcggcgcgg ccccgcgtgg cgcgtgctgt tcctgttctg ggctgccttg ctttcggggt
     2221 tcgtgttggt tcccacctct cagcggttcg tcgtggtggc ggttcccgtg ctctgtcttg
     2281 gttcggcaat tggggtgcag tacgtgacga agcgcctggc tgaggtcggg accggctggc
     2341 ggggccgatt gcgaagctac cgtccacgcc cgagcggtta ctgaaatacc ctagagcttg
     2401 ccgggcaatt cagcagcgtc ccaggtgtag tactgagcgg tgttgggctt gctgccgaga
     2461 tcggtcgcga agtttccggt cttggtggtg tctttgccct tcagaaatcg gtcgatcatc
     2521 gccgtcagcg aggcgtcgta ctgggttcgc cactggcagt gcgagccgga ggcgccctgg
     2581 tacgcaaagt tgtcagctac accgagcgcg gtgaatatgg ctttgccggc catggcggtc
     2641 acatatgccg acttggggtc caagttactg atgccggggt tgtccacgat atagagtgcc
     2701 cggggcgcga tcagtgccat cgcttcgtgc atgtcgaccg gtagccgatc cgtattgtcg
     2761 ccagcagcgt tgtttgctgt ggcaaagggg cccaaggcca cttcggacag ccaacgtacg
     2821 tagctgatgg catgatacgg ccactcggcg gtatcgttcg cgccggggtc tagctgttcc
     2881 ttcaggcgaa ggcccacggt gcctccaatt ccggactcac acggaatcgt gagcgcaatt
     2941 cgattatcga atacgccagc agcgaaggcg ccttttccgt tgcgggagca gccggtcacg
     3001 ccaatgtgat acggatcgat ggtgccgggg ttttgttcaa gaagatcgat gatgcggctc
     3061 acctgccagg cttgagccac catgtagccg gcgggatggt tggcgccgta gaacgtaaag
     3121 aagggacccg acttgttacc cgtgccgcct tgggcctcga tggcgctcat cgtgatcttg
     3181 gcgacgccac ttgggatcgg caaagagctc cccagaccgc cgtcatagat gatgatcgcg
     3241 ggagcgggcg cggtggcgcc gttcatgttc acggtgacgt cgaatgaagc gctcttgcta
     3301 ccttccgtca cttttaccga gatcttgctg gtgctcacgg tgccagaaac ggagcctttg
     3361 gcgggaatcg gtttttcgcc gaaaacgtac ttgaagtgct gttttacgat ctccgctcgc
     3421 cggcacgccc actcactctt gtccttgatg cgagtgccat ccagcttggt gaaaggatcg
     3481 ggtagcttgc cgtccgcctt gagatcagcg taggccggca atgtcgcgtt gacggcgcag
     3541 ccggcactcg gatcctcttc cttgccccaa ttgacggttc cgggagtccc gccgcctacg
     3601 ccggtggtgc cggccgtgtt ggtcccgcca ctacccgccc cgcccgggct cgaaccgccc
     3661 gtggggtttg tgccgcccgc ggcagcgctc gtaccgcctg tcgcagtgtt gccgcccttc
     3721 gcggtgatgc cgcctgtcgc agtgatgccg cccatcgcgg tgttaccgcc cgtcgcggtg
     3781 ttaccgccca tcgcggtgtt accgcccatc gcggtgccgc ccatcgcggt gccgcccatc
     3841 gcgttagtgc cggccgtcgc gctagagccg gccgtcgcgc tagagcctcc cgtcgcgctg
     3901 atcccgccgc tggttgtcgt cccagcgctg ttcgagccac cgttagcccc tggctccttg
     3961 gagccgctgg agcatccgag ttgcatgagg gtcatcacga ccgaaaggtg cccgagcgac
     4021 ttaagagttg atgacctcat cgatattcct ccgcgcaagc gcgttgttct tgcaccaggt
     4081 gctatgccca gcgccggtcc aaaggatctc gagagttcca gcgtcgcccc caagagcgct
     4141 catgaatcac tttggtgtgc tcaaataaga gtggccaagg tgtaatcgtg tgacacccca
     4201 tcgcagtgct ttgacgtcgg gccgtcgtcc tcgtcgttca tgattgcgcc tggtgaggtc
     4261 tccggagcga gcgcacaacg ctgcgacgcg cgtcaggcgt cgaatcgcag ttcggccccg
     4321 cgcagagaag ccgacgccga cgcggggcgc ccgaattcag ggcgaccgag gcgagcagga
     4381 gactggcgcg tgacctcgga tcacttacga gcgcacgatt cacccagctc ggagagcacc
     4441 gtgttgtcgt cgagtagcgc ggccacgtgc gggcaggctc cgtactcgca gccgtgctcc
     4501 gtcgacaagc aggcgctcag aggctgaacg atgtcgcgga tacattccgt gaacccgtcg
     4561 ccacgttctg catggtgctc tagcgcttct cgcactgcga cagcgtcgta gccctcgaac
     4621 tcgcaagagc catcgggaga ctgtacttcc tggcaagtac agagcgcgcg ataagcgttc
     4681 tccagaccgt ccaccgcgtc ttgaactgcc ctcaccgtgg accccgccga gcccgtagac
     4741 cccgcccaac caacagagcc ccccgacccc acagtgctcg cggagccaac ggagctcgtt
     4801 gagccaacag agctcgctga tccagcagag ctcgccgatc caacagagct cgccgatcca
     4861 acagagctcg ctgatccaaa agagcccgta gatcccccag acggcgtgtc cgaagtgttc
     4921 cgatctgccc cgtcgggact accagccttg cccccacagc caagcgcgtt aacgcaaaga
     4981 gcagctgcaa gcgcaagtct gaagcggaag acaggtgcca ttagcagcgg aggataccag
     5041 acagtcgcga gcgaagcctg cgcgccttcg tgcaaatgaa gatcattgcc tttcaaaaga
     5101 agacagtggc gtggaatttt ccactgttgt cggtcgtggc gagctcgttc taacgagcct
     5161 gacactcctg agactggcaa acgccatgcg gtgtgctgat atgcgtccgt gtctcgcttt
     5221 cccgtatccc tcgctgtcac cctcgtccct cttttcacgc taacctcgat cgcttgttcg
     5281 gaaagctcgg gggctcccca gaccgggagc acctcggggg gaacggctct gggaggcgca
     5341 atgggcggag ccgccgggcc aggctccggc ggtacgccaa ccacgggagg caacggcggc
     5401 ggtggttttg gaacggtcga cgcgaaaggt ggcgcttcgg gatctgtcgg cgccacaggt
     5461 ggtgcgagcg gcgcggccat gggaggaagc ggaaacgccg gcggagccag tgccggcatg
     5521 agttcgggag gacaagccgc cgcgggcgcg ggcacgggtg gtttcgcggc aggcgcagct
     5581 ggcgctggag gcagcggcgc cggaggcaac agcgctgggg gcaacggttc aggtggctcg
     5641 tccgcaggcg caactaatac cgggggaggt agcggctccg gaggttcgtc cgtgggtgcg
     5701 ggcggtagcg gcgacagcgg cggttcctct ggaggcgcca gcggcagcgc aatcttgact
     5761 gaaggattcg aaggaaccgc gctccccagc ggctgggaca acttcatcgg ctacgtcaag
     5821 aacgggatga atccgcaggg tgagttcaag atcgtgatcg actcgtcgaa gaagcacagc
     5881 gggtcgagct cggtgcactt tcacgggggc agcacgcccg cgatgctcac ccgccctctg
     5941 cccgcgaaca ccaagaaact gtatgttcga gtttgggtct ggctctcgcg ctctctcgga
     6001 aacaaccccg acggtaatca cgagacccta cttggcattc gcaagaattc ggggacggcc
     6061 gacaacgagg ttcgcttcgg cgaaatcaaa ggcgcgattg gcgtgaacga ggttccaacc
     6121 gacgcgctca gcctcaagcc ggagctctgg aacaagggcc ccaccatcgc gaaggagaag
     6181 tgggtatgtc tcgaggccgc gttcctgggc gataaggcta cgcacgagtt ttatgcttgg
     6241 gccgatggca tggaggtcca caagatcata ggcgcgagcc agtggcatgc cccggttgcc
     6301 gcggacttcc tgaccggcaa attcaatgag ttcatcattg gctggcagag cttcagcaac
     6361 ttcaccgccg atgtttgggc agacgacttg gtgctgagca ccgagcgtgt cggctgcaac
     6421 tgacggcccg atcgagcccc cgttgctccc tgcggggccg tcaacggttt cgatttttcc
     6481 gaacttgtcg aggagcgctg gcatctgcgc tcgcgattcg ctccagcagc tcctcgagca
     6541 agagtcgttc gccggccgag agcacattga cggaatccag gcccgccttg agcgccaccg
     6601 ccgcaccgac ggcacccgta gccaccggtg ggttccgctt tccggcgatg gcatggatga
     6661 ccgattcccg cgcgagatcg gccaacccga gatctcgttc gttcgcaggc tgctcgagca
     6721 aacgtttaat cacgcccacg ccgacggcct gcagcagcgc caaggcccgg tcttcactga
     6781 cccgcagcag gccttgctcg gcgagccgac ggagcttctt ccgaagtact tcctcccccg
     6841 atcgcgcggc gggggtcgtc tggaagcgct ggtcaccatt catgagcgca aacaggctgg
     6901 gttgcgagag cccgaaagcg acgtgctcat cccaaccgct ccggagatcc tggatgggat
     6961 cgggatgcgg tgccctggct cgcttggccg cgacatactt ctccagcgca aactgcgccg
     7021 tggcgtccag caaaccctcc ttgtccccga agagtcggta aatagtggga gcttgcactg
     7081 aagccgccgc ggctaccgcg cgggtggtcg cggcgtcctt gccgccttcg aggattaagc
     7141 gtcgcgcggc ttcgaggatg cgttcgcgga gcgccgtgcg ctgaacttca gtatcgtcgg
     7201 gcggagggac catgtgaggg tgggtgatct gcgctttagc gcgacttcgc ggccacgtca
     7261 ctttcgagcc gaggcgtgct gcgctcagac ctggtttagg cgcggcccaa tccagcttcc
     7321 gacatccagg gttgaccgtg ttaacgtcga tgctattgat ctgttatcaa tgataacatc
     7381 gtctttggca aaagtgtcgt tgaacaaagg aaggccgcgt tcatgatcgt gattacggga
     7441 gcgacgggac agcttggcgg cgccatcgtg cgctcgctgc ttcaacgcgc gccaggggct
     7501 cggattgcgg ccagcgtgcg caatcccgaa caggcgggcg ccttggctca gttgggcgtc
     7561 gaggtgcgtc ggggtgactt ctctcgaccc gagacgttgg tggccgcctt ttcgggcgca
     7621 actcaggttc tcgtggtctc ctcgaacgcc ggggcgttcg gggcggaccc agtcgcccag
     7681 caccgagcgg caatcgacgc cgcaaaacag gcgggtgcgc aacgcattct ctacacgagc
     7741 catgttgcgg cgagcgcctg gtcgtcgttc tcgccaatgc acacgcacgc cgccaccgag
     7801 cggatcttgg ccgagagcgg tgtgcgctgg acatccctac gcaatggctt ctacgccgcg
     7861 agcgccctgg tattcattgg agatgggctc gagaaagggc ggattgccgt gcccgatgac
     7921 ggcaaagtgg tatggaccac tcacgcggat ctcgccgacg cggccgcggc cgtggtcctc
     7981 gacgaagggt gcgacggtcc aattccgctc accggtggag aggggctcga tatggccgac
     8041 atcgctcgca ttgctagcga ggttttgggc cggaacatcg agcgcgagag catttcggat
     8101 gccgcgctcg aagaacgatt gaaagctttg ggggttccgc cgccgagggc cgatatcttg
     8161 ctcggcttct accgagcgag ccgggccggg gagttcgcgc gagtagatgg cacgctcgag
     8221 aagctcattg gtcgcaaacc ccagagcatg agaagcgtac tcgaacggct acccgcttag
     8281 gagcggccct gacgtcgcgt tgcacctgac gggcatgagt gcttgcgctt gcgcgtcgcg
     8341 catcgatcgg tagaactttg cgcatggaga agatcccgac tattttcgag cgcggcgagc
     8401 ggttcaaagt catcaatcaa ccccgcacta gttgtgattg ggtgtttgct gggcagggcc
     8461 gcgccacgga gaaactagat gggatgaact gccgactcac gatccgtacg gggcaggtcg
     8521 tgcgcgtcga gaagcgtaga aatccaagca aactccaaaa ggaccagggc atcgtcgacg
     8581 gttggtatgt cgacgcggac gagcagtcga acgaggacaa gtggattttc gaggccgttc
     8641 gcggcacgga ggtgcgcgaa tggccggacg gcgaacactc cgtggaggca ctcggcccga
     8701 gcatccaggg caatgtactc gggttggcga agcacatttg cgtgccgttc aatctacgaa
     8761 tcccggtcta tgaagatgtt ccccgaacgt atgaggaact caaatcgttt ctcgccac
//
LOCUS       DASTKU010000148        16311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_27962,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000148 DASTKU010000000
VERSION     DASTKU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16311
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27962"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VFQ61_05330"
     CDS             complement(<1..1150)
                     /locus_tag="VFQ61_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET9953899.1"
                     /translation="MAQAVGPEAVELVKSHALSCLGATLARLRGYLRIPAISCDAAHH
                     ADVRRLAGCVQQDLTELGFHSRVLELPGALPLVMAERHDAGPDAPTLLVYGHLDLQPV
                     RGEKWSHDPHDAVVRNGRLYARGAADDMGGWVSHFAAIEAWTSVLTRLPMNVKFVIES
                     EEEIGSPNLERFMDAHPECFSADVMILTDCENPAVDVPGLTVSLRGVFQLELRCEALQ
                     ADIHSGVWGNVAPDAGNALILLLARLLDEDGRLSVGRCELPAQVSQELAQVPLDDRSL
                     RVGSRLLDGIEPLPMRGRSPAEWMWRQPAITVLSTTFPRPEEHKNAVRAKASAALSIR
                     IAPGQKREELVRAIAERVCSHAPAGVRVTLTETPGAAESFLYNPSGPAF"
     gene            complement(1170..1679)
                     /gene="nikR"
                     /locus_tag="VFQ61_05335"
     CDS             complement(1170..1679)
                     /gene="nikR"
                     /locus_tag="VFQ61_05335"
                     /inference="COORDINATES: protein motif:HMM:NF003381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HET9953900.1"
                     /translation="MADKLVRFGVAMEASLLKQFDELVEARGGTRSEAFRDLARAAVV
                     KSRVRRGAEAVATLTLIYDHHVRDLTERLTELQHDLGHRIRSTLHVHLDHHHCLEVII
                     MHGRSDHLQEIADRILALKGVKHGGIELYTGQGSEPEPDEELRIIGKVEPHHHPHPEP
                     AGAVRTRKA"
     gene            complement(1686..1937)
                     /locus_tag="VFQ61_05340"
     CDS             complement(1686..1937)
                     /locus_tag="VFQ61_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953901.1"
                     /translation="MASATPGCSLIADASDIDQACEAGFKYCKDVEACVSYSDPGFGC
                     ALTSCEPCSATSSNQVMGCVRGRCEVVACTTQGDCRQGM"
     gene            complement(2014..2913)
                     /locus_tag="VFQ61_05345"
     CDS             complement(2014..2913)
                     /locus_tag="VFQ61_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953902.1"
                     /translation="MSTWRRRGAHWTKRSLGMALMFGLLNAPVARAEPDLPAEPAVDI
                     GVVEECSRHHGAARLFLLREEWQRARDELQGCLPERCPVSLRSDCADWQAEFERALAT
                     LYVALPDEDGWRGATLSLDGVTQVLVGPPEPIRLTPGAHRLVFSRAGYLPDVHEIVLR
                     KGEARQLNVRWTSNRVATSLDDARPIPATTYWFSVGALTAFASTAVLGGLALSSLSNA
                     RQTCAPTCNPERRRTIGNLAMAADTSAVIGVLLSAFAVASYLGRPPASGTPAASISGA
                     GPRVIPDLKLSKDSVSLSLHREF"
     gene            complement(2910..4304)
                     /locus_tag="VFQ61_05350"
     CDS             complement(2910..4304)
                     /locus_tag="VFQ61_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953903.1"
                     /translation="MPSEAAAWEALVQEHERTHDVFIVTDSPTKFKPDLAGRITTDMM
                     SGAPRGTFQANLGADIFGWCAFEVNDPAGKLSTRSSRIRSDLASVLDAKGRSAGAFKH
                     ELWPDLLRFTYFSPIRDVCEDEACRFEKPLLAIPLNIHRLNLLYYRSKQLAEPPNLDA
                     LCPVEGRPQDLRIAVNQEEYFPLGLLFFENLFLAFLRRDSPSARVTAERYYDFFAGAE
                     GAVDAQGAPDWEKPVRDALQCLHRMSVITLREAGWEKQVNAVAGGAAEVTAMGDWANA
                     HLREAVREHEVIPTPFPGTEEFFVFTSDAFPLPIGARFPDEVEDLLVTLANPASQKRF
                     NDLKGSLPAVGLGPRQQPFDLNRCADFLSPQDEPTSGQGFDQARCRNLKDLHEKMPLL
                     ATSGLIGFPGGELREALLDVIEIGDAESLDRAIGVLARARTAEFAPFQALLATSDIPD
                     WKPPPRHPLASRKE"
     gene            5490..6098
                     /locus_tag="VFQ61_05355"
     CDS             5490..6098
                     /locus_tag="VFQ61_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953904.1"
                     /translation="MQVSETDCRTHSRQGRTTQAIECFEQLSRGNGIQADVATYEVAR
                     LSATSGTDPLQVLSLLDRYQQRFPEGVMRGEVDWLRVQSLYRSGRHRAALSESDALLS
                     TSAGRVLAAEVHWLRGIIFEERLLDFGRAASEFVTFVGMPGARGDEGEWRRARCLERL
                     GRRTEATEAYRRYLERGDARRAAEVRSKLEELADTRRSPLSN"
     gene            6134..6718
                     /locus_tag="VFQ61_05360"
     CDS             6134..6718
                     /locus_tag="VFQ61_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9953905.1"
                     /translation="MAAPTFELNAWLETELLTACVDGDTRAWRELHRRYFPVVVAFLR
                     KLGVRERELEDSTQEVFLQVHKYLPRFRRDSELSTWLYRICITQARSVRRRTHVAESL
                     LRFLSFAPRDLVVSEPSLPEFAERKKIQSALTQLPDAERAAFVLYEMEGLPGKQIALI
                     LNCTEASVWRRLHEARKKFQRAIGLEPRPSSGTE"
     gene            6794..7378
                     /locus_tag="VFQ61_05365"
     CDS             6794..7378
                     /locus_tag="VFQ61_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9953906.1"
                     /translation="MSANPNSHGARRRFVAGLVRRLGTPQCDVEDVVQDVFEIFFRRF
                     QSAESEAAARALLSAISARVCANQRRKSARRKLRLPLHELGAEPDQFAPEEEDLSQEA
                     MLEAKEARSLLTRALSRLDAPKREVLILAEIEALTLREIAVQIRIPCNTVASRLRAAR
                     SNLDRAVRVLSVPPRSSARTRPPGLSQASREVFQ"
     gene            complement(7322..8929)
                     /locus_tag="VFQ61_05370"
     CDS             complement(7322..8929)
                     /locus_tag="VFQ61_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9953907.1"
                     /translation="MFNARYPLPEPAWASLLPSVDTTAVLACFLVLARSAALRRGQAE
                     RRIASAAFWGLAFITFVVRLFRVGDGIAEVFMERHANLFVDAVLLPEVIRLLYSTLPL
                     LQFSLVILGLLICVPLSIWLAYRAWRATARDLAQREVAASFLVITLALGVVGWVRPKP
                     IDHERYARAFSASVLPRVRDDMNVFLQARELRVQREARLRLARARFAESKGDLAKLEG
                     ANVLLFVVESYGVTVLRKATYVPHTEAMYAAFESALSKKGFQVVSNLLDSPTTGGGSW
                     LAHASMLSGMRVADQDDYQFMVQAEPRSLVWFFAEAGYYTVSAEPGTQRQSADPYHFE
                     QRIWGPKFGYRGPKFTWAPMPDQFIIDRIHRTEVLDHPEPKFIKYTLVSSHAPWSQLP
                     PVLENWNDVGDGSIYRSLPKTQFPTSWTNLSQAQLPYMRSVEYSMEVIRRYLEEFVDD
                     DSLVIVLGDHQPSALVTDRSPDMSVPIHIVSRNRDFLRKFEKRGYVPGMKPALNRRRV
                     RPLESFPGDLLEDFSTRLGEAGRAGTR"
     gene            9379..9714
                     /locus_tag="VFQ61_05375"
     CDS             9379..9714
                     /locus_tag="VFQ61_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953908.1"
                     /translation="MLLAALALGACGATDGEAPVADHVEQAIANQEVLYTYYDCAHLD
                     QVVGTRFHACVATNAWSQDGRTSRCFEVESFDCSNEHGTLQYCEFDVCAAYDPFGSIP
                     AFTEWAQSN"
     gene            complement(9774..11108)
                     /locus_tag="VFQ61_05380"
     CDS             complement(9774..11108)
                     /locus_tag="VFQ61_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953909.1"
                     /translation="MSAARATESSHLRLPRSAAIGLVVSMAGSRVGLTALSLVALMSC
                     QERHASRSSTKGAASASGVSASNVSASSTAQPGSATQSSAACLKGGGLVVKDTTLTRA
                     CSPYELARGLQVLDGATLTIEPGVELRFAADQGLEVGAADGRAGRLVAEGSAEAPITL
                     VAAGQTRADAKPQDSAWRGIRFYSGTLPGSVLAHARVSGAGLSQTAGAAAEEAGCVTV
                     TGVKHGALRLVQLQLESCNPAALVLRGGGVQLSGLHFAHSSVGLSSDAQSAAALPTDI
                     EYREVANNVITGGVISDHTVWGPQGRPFDVRGPVTVGGANRPALILQAGVELRFGAEA
                     GLLVGTQGPGVLKAMGTRESPVKLQPLANGGAWRGLRFGAETLPGSSLEFTELSGADG
                     GVRIETPPKALSIQDSTLANNAVDVAVVCRSEPKLTRVRYTSKRGLVREPCK"
     gene            complement(11105..12064)
                     /locus_tag="VFQ61_05385"
     CDS             complement(11105..12064)
                     /locus_tag="VFQ61_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953910.1"
                     /translation="MKSLGRSCISKGRWLALCVMAIGCHAPRPARVAHGSPGAPCGSL
                     QTEPCEEGTAEAGYSAPGKHLPGVLSTCEKYATLKHGRYEFMNNVFGAERAQGFEQCL
                     LRRERSGKTELGWTWAWPASDAHGFAFPEVVFGWKPWSPKSTYAKLPVRLEDIESLRL
                     RYAVETEKTGRASLAVGMWMTNSNQANPTTIVTETVIWLDSPEDAKPPGERVAALQVE
                     GADYELWHDPNHGDRGNGQGWDTYYFKRRSRHDGSPRRGTCKLHAFLQTLSKREAIRD
                     DWFLASVEFGEEALSGRGTTWVRDFEVSLDKRDLAGKRVKRDP"
     gene            complement(12137..13087)
                     /locus_tag="VFQ61_05390"
     CDS             complement(12137..13087)
                     /locus_tag="VFQ61_05390"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF024801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9953911.1"
                     /translation="MGRLVDGKWTTTTLRADPQGRFLREATRFRNQIRADGSTPYSPQ
                     RGRYHLYVSLACPWAHRTLLARHLRGLESVVSLSVVHPHMGENGWEFRDGAGCSADHV
                     NHARFLREIYVKSDPHYTGTVTVPVLWDIQTSSIVCNESRLILRMLNRAFEPFAQGTA
                     NLSPPELEADLDREMDALYESVNNGVYRVGFARTQVAYDEAVSQLFKALDHYEARLSQ
                     SRYLFGATLTEADLCLFTTLLRFDVVYHYHFKCNLRRLTDYPNLWGFTRDIYQLPGVS
                     EICNLDHIKQHYYTSHPDLNPRGIIPVGPTIDLDAPHGRS"
     gene            complement(13248..15017)
                     /gene="thiC"
                     /locus_tag="VFQ61_05395"
     CDS             complement(13248..15017)
                     /gene="thiC"
                     /locus_tag="VFQ61_05395"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HET9953912.1"
                     /translation="MRTLPLQGAYNPSSRSEGTNPGSFARRPDIGGPLSFASPDTPGM
                     PQPSPKTAWDFLPSGFERGPDGLRVPAGYVPVTQLEKARLGIVTPEMRRVSEREPHLS
                     PEQVREEVATGRMIIPANRVHLSYGLDPMCIGRASRTKINANMGASPVSSSTEEEVEK
                     LHWSVRWGADTVMDLSTGGDLDRCREAIIQNSKVPIGTVPIYSMIIGRRIEDLTEKEI
                     LAGILHQAKQGVDYFTIHAGVLREHLPFVQKRLIGVVSRGGSLLAKWMLVHGRQNPMY
                     TLFDEICGIMREYDVSFSLGDGLRPGGLADASDEAQLRELETLGELTERAWKQGCQVM
                     IEGPGHVPFDQIEYNMKLERQRCHGAPFYVLGPLVTDVFPGYDHITSAIGATAAAYHG
                     ASMLCYVTPKEHVGLPKKDDVKQGCIAYKIAAHAADVALGIPGTRDWDDSLTKARAAL
                     NWEKHFELAFDGDTARAFHDEDLDVDTDFCGMCGHDWCSVRISKEINEFASGKEAGFE
                     RGAARVSQPLSPSQREILERRGVLSPEELTRLATKTAKAVGVSRGKKAGCHSDLGDGS
                     DARQVQDQELVALRRKDQAPAGA"
     regulatory      complement(15049..15142)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15280..15831
                     /gene="yjgA"
                     /locus_tag="VFQ61_05400"
     CDS             15280..15831
                     /gene="yjgA"
                     /locus_tag="VFQ61_05400"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HET9953913.1"
                     /translation="MATSSRIFTLDPPPEPDSDLPSRADKKREVREREERLMSLARQI
                     VELSPRLTTKLELPDLLLEAVDHARRIPSAPAQYRALRKVRTLLRDDDVPELERRLAD
                     LLDPSRVNPSTEAVAHWLARLIGEGEPALDAIVEQYPNADRRQLRQLSRNVTRASEAN
                     RAAAEQALKKVLRDVIRGAPGAT"
     gene            15839..>16311
                     /locus_tag="VFQ61_05405"
     CDS             15839..>16311
                     /locus_tag="VFQ61_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953914.1"
                     /translation="MKCSLVALLLLASCFSPRLSPPASDRAPRKYERTDSTSKVLEVE
                     SYVWTSTDAATCETPRAPPSAARFAALPAESSASSASPASGVDTTNSATPGSIADAAK
                     TVEGMRPGFNACYKDFLRRSGRHGHSGAIRLRLDVNCVGAVSRLRADAYGLDRRA"
BASE COUNT         3129 a         5397 c         4934 g         2851 t
ORIGIN      
        1 cgaaagctgg ccccgagggg ttgtacagaa agctttccgc ggccccaggc gtttccgtca
       61 acgtcacccg caccccggcg ggggcgtgag agcagacacg ctcggcaatc gcgcgcacca
      121 attcttctcg tttctgccca ggggcgatgc ggatcgagag tgccgccgac gccttggcgc
      181 ggaccgcgtt cttgtgttcc tcgggtctcg gaaaggtggt cgagagcacc gtgatcgccg
      241 gttgccgcca catccactcc gccggactgc gcccccgcat cggcaggggt tcgatcccgt
      301 cgagcaagcg ggagcccacg cgcagcgatc gatcgtcgag cggtacctgc gccagctcct
      361 gactgacctg cgccggcaat tcacagcgcc caacgctcag gcgcccgtct tcgtcgagta
      421 gccgcgcgag caacaggatc aacgcgttgc ctgcgtcggg cgccacgttg ccccaaacgc
      481 cggagtggat gtccgcctga agcgcctcgc agcggagctc gagctgaaac acgccgcgca
      541 agctcacggt caagccgggc acgtctacgg cggggttctc acagtcggtc aagatcatca
      601 cgtcggccga gaaacactcc ggatgcgcat ccatgaagcg ttcgagattc ggcgagccga
      661 tctcctcctc gctctcgatc acgaacttca cgttcatggg caggcgcgtc aacacgctcg
      721 tccaggcctc gattgccgcg aagtgcgaca cccaaccgcc catgtcgtcc gccgcgccgc
      781 gcgcgtagag tcgcccattt ctcaccacag cgtcgtgtgg atcgtggctc catttttcgc
      841 cacgcacggg ctgtaagtcc aagtgcccat aaaccagcaa ggtgggcgca tcgggcccgg
      901 cgtcgtgtcg ctccgccatc acgagcggta acgcacccgg cagctcgagc acgcgcgaat
      961 ggaacccgag ctcggtcaaa tcctgctgta cgcagcccgc caagcggcgc acgtcggcgt
     1021 ggtgcgccgc atcacaggat atcgccggaa tgcgcaagta accgcgcaaa cgagcaaggg
     1081 tcgcgcccaa gcagctgagc gcatggcttt tgacgagttc gacggcttcg ggtccgacag
     1141 cttgggccat gctttcctct cgattgcgtt caagccttac gagtgcgaac cgcgcccgca
     1201 ggctccgggt gcggatggtg atgaggttcg acctttccga tgatgcgcag ttcctcgtcg
     1261 ggctccggct ccgagccttg gccggtgtaa agctcgatgc cgccatgctt gacgcccttc
     1321 aaggccagaa ttcggtcggc aatctcctgc agatgatcgg accgcccgtg catgatgatc
     1381 acctcgaggc agtggtgatg gtcgagatga acgtgcagag tcgaacgaat tcggtgtcct
     1441 agatcgtgct gcagctccgt gagccgctcc gtcaaatcac gaacatggtg gtcgtaaatc
     1501 agagtcagcg tcgcgaccgc ctccgcgccg cggcgcacgc gggacttgac cacggccgct
     1561 cgcgccaggt cccgaaaggc ctccgagcgc gttcccccgc gggcttccac gagctcatcg
     1621 aattgcttga ggagggaggc ctccatggcc acgccgaagc gcactagttt atcggccatc
     1681 cgccgttaca tgccttgacg gcagtctccc tgagtggtgc aagccaccac ttcgcagcgg
     1741 ccacggacgc agcccatgac ctgattgctg gacgtggccg agcacggctc gcacgaggtg
     1801 agcgcacagc caaaccctgg gtccgagtag ctgacgcacg cctccacgtc cttgcagtac
     1861 ttgaaccccg cttcacaagc ttgatcgata tccgaagcat cggcgattag cgagcaaccc
     1921 ggcgttgccg acgccacgat taggaccgca cacaagccta gcgagcggga cagcacgcga
     1981 cgcaaggccg ttgatctcga gctcggcttc acttcaaaat tccctgtgca aggacaagct
     2041 cacgctgtct ttggacagct tcaaatccgg aatgacccgc ggccctgccc ccgagatgct
     2101 cgcggccggc gtccccgatg cgggcggccg tcccagatag gaagcgaccg caaacgcaga
     2161 cagcagaacc ccaatcaccg ccgacgtgtc agccgccatg gccaggttac caatcgtgcg
     2221 ccggcgttct gggttgcacg tgggcgcgca ggtttggcgg gcattggaca atgacgacaa
     2281 cgcgagccct cccagcacgg cggtgctggc gaaggccgtc aaggcgccaa ccgaaaacca
     2341 ataagtggtc gctggaatcg ggcgcgcgtc gtcgagactg gtggccactc gattcgacgt
     2401 ccagcggacg ttgagttgcc gagcctcgcc cttacgcaac acgatctcgt gcacatccgg
     2461 caggtatcca gcgcgcgaga acaccaaccg gtgggcgccg ggcgtcagcc ggatcggctc
     2521 cggcggcccg acgagcacct gcgtgacgcc atccaaagag agcgtggcgc cccgccaacc
     2581 atcttcgtcc gggagagcga cataaagcgt cgcgagagcg cgctcgaact ccgcctgcca
     2641 gtccgcgcaa tcggagcgca aagaaaccgg acagcgctcg ggcaaacacc cctgcagctc
     2701 gtcacgagcg cgttgccact cttcgcggag caagaacaaa cgcgcggctc catggtgccg
     2761 tgaacactcc tccaccacac caatgtcgac cgcgggctcg gcgggaaggt ctggctcggc
     2821 gcgcgccacg ggggcgttca gcagtccgaa catcaaagcc atgcccaagc tccgcttggt
     2881 ccagtgcgcg ccgcgccgcc gccaggtgct cattcttttc tcgacgccaa tgggtgacgc
     2941 ggcgggggct tccaatcggg gatgtcggac gtggccaaca gcgcttgaaa gggggcaaat
     3001 tccgcagtgc gggcgcgggc caagacgcct atcgcgcgat cgaggctctc tgcgtcgccg
     3061 atctctatga cgtccagcag cgcttctctg agctcacctc cagggaaacc gatcaagccc
     3121 gaggtcgcaa gcaaaggcat cttctcgtgc aggtccttga gattccggca gcgcgcctga
     3181 tcgaagccct gcccggaggt tggctcgtct tgtggactca agaagtcggc acaacgattc
     3241 agatcgaagg gctgctggcg aggaccaagc cccaccgcgg gcagggagcc cttcagatcg
     3301 ttgaagcgct tttggctcgc gggattcgcc agcgtcacga gtaagtcctc cacctcatcg
     3361 gggaatctcg ctccgatcgg cagagggaag gcgtcggatg taaagacgaa gaactcctcc
     3421 gtgccgggga acggcgtggg gatcacttcg tgttcgcgca cggcctctcg caggtgagcg
     3481 ttggcccagt ctcccatggc ggtcacttcg gcggcccctc cggccaccgc gttgacttgc
     3541 ttttcccagc ctgcctcgcg cagcgtaatc accgacatgc ggtgcaggca ctgaagcgcg
     3601 tcccgcactg gtttttccca atccggtgcg ccttgcgcgt ccaccgctcc ctcggcgccg
     3661 gcgaagaaat cgtaatagcg ctccgccgtc actcgcgccg acggcgagtc gcgcctcaga
     3721 aaagctagga acaagttctc gaaaaacagc agcccaagcg ggaagtactc ctcctggttc
     3781 accgcgattc gtaggtcctg cgggcgccct tccactggac acagcgcgtc taggttcggc
     3841 ggctccgcca gctgcttgga ccggtagtag agcaaattga ggcgatggat gttcagtgga
     3901 atggcgagca gcggcttctc gaaccgacac gcctcgtcct cgcacacgtc tcggatcggc
     3961 gagaaatagg tgaaacggag gagatctggc cagagctcgt gcttgaaagc ccccgccgac
     4021 ctgccctttg cgtccaacac gctcgccaag tcggagcgaa tgcgcgaact ccgggtgctc
     4081 agcttgcctg cagggtcatt cacctcgaac gcacaccagc caaaaatgtc ggcgccgagg
     4141 ttggcctgga aggttccccg aggagcgccc gacatcatgt ccgtggtgat gcgcccggcg
     4201 agatccggct tgaacttggt gggactatcc gtgacgatga agacgtcgtg agtccgctcg
     4261 tgctcttgaa cgagcgcttc ccatgctgcg gcctcacttg gcaattgcca ccagttgtgg
     4321 acggtgatgg aatgaactgg tttgtctggc tcgctgcacc ctagaatgct gcatcctaga
     4381 atgcagagcc caactaaaga tgcgagccag cccgggcctg ctcggccaga gcgcgtcgaa
     4441 atcccagagc gcgtcgaaat cccagagcgc accgatatac caaggctacg agatgccgct
     4501 ccgcagagca cagacacgaa gcgaacgagc agacgagagc gcacctcatg actctctctc
     4561 ttcgctcctg gctgaaccat ttccgtcatg cgcgacgctt caacgtcgga tattagctac
     4621 aaccgggggg cccgcctacg gcgttcatca ccctcaaaaa aaactagcga aggtccagca
     4681 gcctcgagcc atctaacgta atgccgatcg atctggccac gatcgccctc atcgggggtc
     4741 gcccccgagc ggcggctagg ccataagcag ccagaacgcg aaatagtccg ccaagaacgc
     4801 gagataagca gcgagattaa gcggcgagat aactagataa gcagagctca agcgctatgg
     4861 attcaggcac gaaacacgaa gcaccccggc tggccggcga gggaggagcc atcggctcgc
     4921 tcctcagaag tgccaattcc caatacggcg agggcttgaa cgaacaagcc tgcttcgacc
     4981 ggctcgagaa agcacgcgtc cggaggcagt ggcaagcacg gggtgccctg atggctgccg
     5041 cggccggttg cgcctggttt gccttgaacc cagtcatgcg cggatcgatt ccgacggtct
     5101 cggagcacga aatccgcgct gaattgtgga ataagcccca ggccaggcca tcggcttcga
     5161 gcgccaagcc gagccacgaa tcatctgggt caccggcaga ggcccggttg gcagaaccgt
     5221 gggcccgaaa cgagagcgcg cggcccagca cgccaacgca atccgagaag ctcatcaatt
     5281 caccagccgg tactcgacgt caggccgaaa cgcgttccgt cgcgccgcag tcagaaccgg
     5341 cgcgagcgat ccaggccggc ttggaacctg cgtcagcgcc ggccacggcc cttccgcaaa
     5401 gggtcgatgc cacaacgccg gcctccgctc ctttcgaggg acgagaagag acaatcccga
     5461 aaccgcttcc ccccgcggat cagggtacga tgcaagtttc ggaaacggac tgtcgtacgc
     5521 acagccgcca ggggcgcacc acgcaagcca tcgaatgctt cgaacagctg tcccgcggca
     5581 acggcatcca agcagacgtt gcgacatatg aggtcgcccg cctcagcgcc acttccggga
     5641 ccgacccttt gcaggtactc tcactgctgg atcgctatca gcagcgattc ccagaagggg
     5701 tcatgcgcgg cgaggtcgat tggctgagag ttcaaagtct ttatcgctca ggtcgtcatc
     5761 gtgcggcgct cagcgagagt gacgcgctgc tgagcacgtc tgccggacgg gttctcgccg
     5821 cggaggtgca ctggttacgc ggaatcattt tcgaggagcg actgctcgac ttcgggcgcg
     5881 cggcgagcga gttcgtcacc ttcgtgggca tgcctggcgc tcgtggagac gagggggagt
     5941 ggcggcgcgc gcgttgcctg gaacggctcg gacgccgaac ggaggcgacg gaagcctatc
     6001 gccgctacct cgagcgcggt gacgcgcgcc gggccgcgga agtacggagc aaactcgaag
     6061 aactagcgga cactcgccgg agcccgctct cgaactgagc gcatcgcagg aggaagaaga
     6121 ggaggacgca gaagtggctg cccccacatt cgaactcaac gcttggctcg aaacggagct
     6181 cctgacggcg tgcgtcgacg gagatactcg cgcgtggcgt gagctccacc ggcggtactt
     6241 tccggtcgtg gtggcgttcc tccgcaagct cggcgtcaga gagcgtgagc tcgaagactc
     6301 tactcaagag gtgttcctac aggtccacaa gtacttgccg cgttttcgtc gagactcgga
     6361 actttcgacc tggctctacc gaatctgcat cacccaggcg cgcagcgtgc gtcggcgtac
     6421 ccacgttgcc gagagcctgc tcaggttcct ctcgttcgcg ccacgtgatc tcgtcgtctc
     6481 cgaaccgtct ttgcccgagt tcgcagagcg caaaaaaatc caatctgcac tgactcaact
     6541 cccagacgcg gaacgcgcgg cgttcgtgct gtacgagatg gaagggctgc ctggcaaaca
     6601 gatcgccttg atcctgaatt gcacggaagc gagcgtttgg cgtcgcctcc acgaagcccg
     6661 taaaaagttc cagcgcgcca tcggtctcga acctcggcct tcgtcaggca ccgagtagga
     6721 gaaagatccg aaagcgacgc tcgagtctcc agtcgagcga aggttcgaaa tagctctccc
     6781 ctctaactcg ggcgtgagtg caaatccgaa ctcgcatggg gcacgtcgcc gcttcgtcgc
     6841 ggggctggtg aggagactcg gcacaccaca atgtgatgtg gaagacgtcg tgcaggacgt
     6901 gttcgagatt ttcttcagac gcttccagtc ggccgagtcc gaggcggccg cacgcgccct
     6961 gctcagcgcc atttccgccc gcgtctgcgc gaaccagcga cgcaagagcg cgcggcgcaa
     7021 gctgcgctta ccactccatg agctcggcgc tgaaccggac cagttcgctc ccgaggaaga
     7081 agatttgagc caagaggcca tgctggaggc caaggaagcg cgctcgttgt tgacgcgcgc
     7141 actctcgcgc ttggatgcgc ccaagcgcga ggtgctgatc ctggcggaaa tcgaggccct
     7201 gactttgcgg gagatcgcgg tccaaattcg aattccgtgt aatacggtcg cctcgcgcct
     7261 gcgcgcggcg agatccaact tggaccgtgc ggtgcgtgtg ttgagtgtac cgccgcgttc
     7321 ctcagcgcgt acccgcccgc ccggcctctc ccaggcgagt cgagaagtct tccagtagat
     7381 cgccggggaa actttcgagc gggcgaacgc gacgacgatt caaggccggc ttcatgccgg
     7441 gcacatagcc ccgcttctcg aacttccgga ggaaatcccg attgcgactc acgatgtgga
     7501 tggggaccga catatccggg ctgcgatcgg tgacgagcgc gctgggctgg tggtctccca
     7561 gaacaatcac caggctgtcg tcgtccacga actcttcgag gtaacgccgt atcacctcca
     7621 tgctgtactc gacggaacgc atgtacggca gctgggcttg cgaaaggttc gtccaagacg
     7681 tcgggaattg ggttttcggc aacgagcgat aaatcgaacc gtccccaacg tcattccaat
     7741 tctcgagcac tggcgggagc tgactccacg gcgcgtgact cgataccaga gtgtacttga
     7801 tgaacttggg ctcggggtgg tccagcacct cggtccgatg gatgcggtcg atgatgaact
     7861 ggtcgggcat gggggcccaa gtgaacttgg gtccacggta gccgaacttg ggcccccaga
     7921 tgcgctgctc gaagtggtaa ggatcggccg attgacgctg agtgccgggc tccgcgctca
     7981 ccgtgtaata gccagcctca gcgaagaacc aaaccaggct gcggggctcg gcctgcacca
     8041 tgaactggta gtcgtcttgg tccgcaaccc gcatcccact cagcatgctc gcatgcgcga
     8101 gccatgagcc gccaccagtg gtcggcgagt ccagcaggtt cgagacgacc tgaaatcctt
     8161 tcttcgacag ggccgactcg aaggcagcgt acatggcttc ggtgtgcggc acgtaggtcg
     8221 ctttgcgcag caccgttacg ccgtacgact ccacgacgaa caagagcacg ttggcccctt
     8281 cgagcttggc gagatctcct ttgctctctg cgaaccgcgc gcgcgccaat ctgaggcgag
     8341 cttcgcgctg aacgcgtagc tcgcgcgctt gcaggaacac attcatatcg tcgcgcacgc
     8401 gcggcagcac actcgcggaa aaggcccgcg cgtagcgctc gtggtcaatg ggcttgggcc
     8461 tcacccatcc caccacgccc aacgccaggg tgatcaccaa gaacgaagcc gctacctcac
     8521 gctgcgcaag atcgcgagcg gtggcgcgcc aagcacggta ggcgagccaa atcgagagcg
     8581 gaacacaaat cagaaggccg aggatgacca gcgagaactg taggagcggc aacgtcgaat
     8641 acagcagccg gatcacctcg ggcagcaaca cggcatccac gaacaggttg gcgtgtcgct
     8701 ccatgaacac ttccgcgatg ccatctccca cgcgaaacaa ccgcaccacg aaagtaatga
     8761 acgcgagccc ccaaaacgct gcgcttgcaa tgcgccgttc agcctggccc cggcgcagag
     8821 cggcactccg cgcgagcacc aagaagcacg ccaggacagc cgtcgtatcg acagagggta
     8881 aaaggctcgc ccacgctggt tccggcagcg gatacctggc gttgaacatg acgttgagaa
     8941 atgaccacgc gaggagaaag cgtaacggcc ccaaccaacg ctggtgacgc gcagaccaat
     9001 cgcgccatgc cgcgaacccc cggtgaatgg cgatcgagcg taggccagcg aagttgagca
     9061 tgctgaatga agtgcggagt ttgcaccgat tcgaggggga agccaccgcg agtcgcgtgc
     9121 ttttgatggg gatgcctcca aggcgcccgc cgcagccgcc gtcacctacc cgttcgggcc
     9181 cagtgagccg tttggaaacc agcacgactc ttcgcgatgt aaccgcatgc acgcgcgttc
     9241 gtacagcgcg ggagtacggc atccacgccg tgcgaagagg gagctaccac catgcccgat
     9301 ctattcagcc gagttgcgtc gctttcaccc cgttcgtcac gctcatcccg aggctcgaac
     9361 ctcgcccgaa ctgttccaat gcttttggcc gcccttgcgc tcggcgcgtg cggcgccacg
     9421 gacggcgaag cgcccgtggc cgatcatgtg gagcaagcca tcgcgaatca ggaagtcttg
     9481 tacacctact acgattgcgc gcacctggac caagtcgtcg ggacccgttt tcatgcctgt
     9541 gtggctacca acgcttggag ccaagacggt cggacgtcgc gctgcttcga ggtcgagagc
     9601 ttcgactgct cgaacgagca cggaacgctc cagtattgcg agttcgacgt gtgcgcggct
     9661 tacgatccat tcggcagcat ccccgctttc accgagtggg ctcaatccaa ctgaccgaac
     9721 gaggaggcct cggactggag gagctcggca gcccgccgca tcggcgatcg agttcacttg
     9781 cacggttcac ggaccaaccc gcgtttggac gtgtaccgca ctcgtgtcag cttgggctcg
     9841 ctgcggcaga ccaccgccac atccaccgcg ttgttggcca acgtggaatc ctggatcgag
     9901 agcgctttcg gaggcgtttc gatacgaacg cctccgtctg ccccgctcaa ctcggtaaac
     9961 tccaggctac ttccaggcag agtctcggcg ccgaaccgaa ggccacgcca ggccccacca
    10021 ttcgcgagcg gctgtagctt cactgggctc tcgcgcgtcc ccatcgcctt gagtacaccg
    10081 ggcccttggg ttcccacgag caggcctgcc tccgcgccaa agcggagttc gacgccggcc
    10141 tgcaaaatta gggctgggcg attcgctccg cccaccgtca caggccctct gacgtcgaaa
    10201 ggcctgcctt gcggccccca aaccgtgtga tccgaaatga cgccgcccgt aatcacgttg
    10261 ttcgcgacct cacgatattc gatgtccgtc ggcaacgcgg ccgcggattg ggcatcgctc
    10321 gatagcccca cgctgctatg ggcgaaatgc aagccgctca actgcacgcc gcccccgcgc
    10381 agcacgagcg cggccggatt acaggactcg agctgcaact gcacgagccg aagcgcgcca
    10441 tgtttgacgc cagtcacggt gacacacccc gcttcctcgg ctgcagcacc agcggtctgg
    10501 ctgagccccg ctccgctcac gcgggcgtga gccagaacgc tgcccggcag agtcccagag
    10561 tagaagcgga tgccgcgcca tgcactatcc tgcggcttgg cgtccgcgcg ggtctggcct
    10621 gccgcaacca acgtaatggg agcttccgcg ctgccctcgg caactaggcg ccccgcgcgg
    10681 ccatccgccg cccctacctc cagcccttgg tcggccgcga atcgcagctc gacgccgggt
    10741 tcgatcgtca acgtcgcgcc atccagaacc tgcaaacccc tcgcgagttc gtaaggacta
    10801 cacgcgcgag taagcgtcgt atccttcacc acgagccctc cacccttgag gcacgccgcg
    10861 ctgctttgcg tcgctgaacc gggctgcgcc gtgctcgaag cggatacgtt cgaagcggac
    10921 acgcccgaag ctgacgcggc gcccttcgtc gaggaacgag aggcgtgccg ctcctgacag
    10981 ctcatcaacg ccaccaggct caaagccgtg agccccacgc gcgacccggc catcgaaacc
    11041 accagcccga tggcggctga acgagggagc cgcaggtggc tcgattcggt agctcttgct
    11101 gcgctcatgg gtctctcttc accctttttc ctgccaaatc gcgtttgtcc aagctgacct
    11161 cgaagtctcg aacccaagtc gtgcctcggc cactcagagc ctcctcgccg aactcgacgc
    11221 tggccagaaa ccaatcgtca cgaatggcct ccctcttcga tagggtctgg aggaatgcgt
    11281 gcagcttgca cgtgcctcga cgtgggctac cgtcgtgtcg gctgcgtcgc ttgaagtaat
    11341 acgtatccca gccctggcca ttaccccgat ccccgtgatt ggggtcgtgc cacagttcat
    11401 aatctgctcc ctccacctgg agcgcggcca ctcgttcgcc gggcggcttg gcgtcttccg
    11461 gagagtccag ccagatcacg gtttcggtga cgattgtggt gggattcgct tgattcgagt
    11521 tcgtcatcca catcccaacc gccaagctcg cgcggcccgt cttttcggtc tcgaccgcgt
    11581 aacgcaaacg caaactctcg atgtcttcga ggcgcaccgg cagcttggcg taagtcgatt
    11641 tcggagacca cggcttccac ccgaacacta cctctgggaa agcaaagccg tgtgcatcgg
    11701 atgctggcca agcccacgtc caaccgagtt cggtctttcc ggaccgctct cggcggagca
    11761 agcactgctc gaacccctgc gcccgctccg cgccaaacac attgttcatg aactcgtagc
    11821 gcccgtgctt caacgtcgcg tacttctcgc acgtgctcag cacaccgggg aggtgcttgc
    11881 ccggcgcgct gtagcccgcc tcggcggtgc cctcttcgca aggctccgtt tgaagcgaac
    11941 cgcacggcgc cccgggcgat ccatgcgcta cgcgcgccgg ccgcggagcg tgacaaccga
    12001 ttgccatcac gcacaaggcc aaccatctac ccttcgatat gcagcttcga ccgagactct
    12061 tcacgcaaaa aacctgctcc gacgcggccc caagatttgc accaacccgc ggcctacacc
    12121 aggcggcggc agtcgattag cttcggccgt gcggtgcgtc caggtcgatc gtgggaccca
    12181 cggggatgat gccccgtggg ttcaagtccg gatggctcgt gtaatagtgt tgtttgatgt
    12241 ggtccagatt gcagatctcc gaaaccccgg ggagttggta aatatcgcgc gtgaagcccc
    12301 agaggttcgg gtagtcggtg agccgacgca ggttgcactt gaaatgatag tgataaacca
    12361 cgtcgaagcg cagcagcgtc gtgaacaggc acagatcggc ctcggtcaac gtcgcaccga
    12421 atagatagcg actctggctc aggcgagcct cgtagtggtc cagcgccttg aagagctgac
    12481 tcaccgcctc gtcgtacgcc acctgagtcc gcgcaaagcc gacgcggtac acgccgttgt
    12541 tgacggactc gtatagcgcg tccatctcac gatccagatc ggcctcgagc tcgggcggtg
    12601 acaaattcgc ggtgccctgc gcgaaaggtt cgaaggcccg gttgagcatc cgcaaaatca
    12661 agcgggactc attgcagacg atgctcgagg tttgaatgtc ccacagcacc ggcaccgtca
    12721 ccgttccggt gtagtgcggg tctgatttga cgtaaatctc tctcaggaat cgcgcgtgat
    12781 tgacgtggtc tgccgagcaa cccgcgccat cccgaaattc ccacccgttc tcgcccatgt
    12841 gggggtgaac cacgctcagc gagacaacgg actccaagcc tcgcaagtgt ctcgccaaca
    12901 gcgtgcggtg tgcccaaggg cacgcgaggc tgacgtacag atggtatcgc ccgcgttgag
    12961 gggaatacgg ggtggatccg tcggcgcgaa tctggtttcg aaatcgggtt gcttctcgca
    13021 agaagcgccc ttgagggtcg gcgcgcaggg tcgtggtcgt ccatttgcca tcgacgagtc
    13081 tgcccatgcc cgttacttca gaggtttgcg gtgcagaagt aaagagggaa ggccgcagcc
    13141 tcccctcttc ccgctcgacg aattttcttg attcgattgg aggtcacccg ggtggaaccg
    13201 tttacgacct ccatgctcga acctgaaacc gcgtagattc agcccgatca cgcgcccgcg
    13261 ggggcttgat ccttccggcg taacgccacg agctcctgat cctgcacctg gcgcgcgtcc
    13321 gatccatcac ctaggtcgct atgacagcct gcctttttgc ctcgggatac gccgacggcc
    13381 ttggcggtct tcgtcgccaa gcgcgtcaac tcctcgggcg agagcacgcc ccggcgctcg
    13441 agaatctcac gttgcgaagg actcaaaggc tgcgagacgc gggccgcccc acgctcgaag
    13501 ccagcctctt tgccgctcgc aaactcgttg atctctttgg aaatgcgcac cgaacaccag
    13561 tcgtgtccgc acatcccaca aaagtccgtg tccacgtcca agtcttcgtc gtggaaagcc
    13621 cgggcggtat cgccgtcgaa agcgagctcg aagtgcttct cccaattcag agccgcgcgc
    13681 gccttggtca ggctgtcgtc ccaatcacgc gtgcccggga tgccgagcgc cacgtcggcc
    13741 gcgtgcgcgg cgatcttgta ggcaatacaa ccttgtttga cgtcgtcctt cttgggcaaa
    13801 ccgacgtgct ctttgggcgt cacgtaacac aacatcgaag cgccgtgata cgccgccgcg
    13861 gtggcgccga tcgcactcgt gatgtgatca taccccggga atacgtcggt cacgagtggc
    13921 cccagcacgt agaacggcgc gccgtggcag cgctgccgct cgagcttcat gttgtattcg
    13981 atttgatcga atggcacgtg tccgggccct tcgatcatca cctgacagcc ttgcttccaa
    14041 gcgcgttcgg tgagctcccc caacgtctcg agctcgcgca gctgggcctc gtccgaagca
    14101 tcggcgagcc cgccggggcg gagcccatcg cccagcgaga aggacacgtc gtattcgcgc
    14161 atgatgccgc aaatctcgtc gaacagcgtg tacatcgggt tttggcggcc gtgcaccaac
    14221 atccacttgg cgagcaatga gccgccgcgg ctcaccactc caatcaagcg cttctgcacg
    14281 aacggcaggt gctcacgcag tactcccgcg tgaatcgtga agtaatccac gccttgcttg
    14341 gcctggtgga gaatgccggc caagatttcc ttctcggtca ggtcttcgat gcgccgccca
    14401 atgatcatcg agtaaatggg gacggtgcca attggcacct tcgagttctg gatgatcgcc
    14461 tcgcgacagc gatccaagtc gcctcccgtc gacaggtcca tgaccgtatc ggcgccccag
    14521 cgcaccgacc aatgcagctt ctcgacctct tcctcggtgc tggatgaaac cggcgaggcg
    14581 cccatgttgg cgttgatctt ggtgcggctc gcccggccga tgcacatcgg atcgaggccg
    14641 tagctcaggt gtacgcgatt ggcggggatg atcatacgtc ccgtcgccac ttcttcgcgc
    14701 acctgctcgg ggctcaggtg cggctcgcgt tcgctcacgc ggcgcatctc cggcgtcacg
    14761 atgcccaggc gcgctttctc gagttgcgtg accggcacgt atccggctgg cacgcgcaat
    14821 ccatcgggcc cgcgctcgaa gccgctcggc aagaagtccc acgccgtctt tgggctcggt
    14881 tggggcatgc cgggcgtatc cggcgaagcg aacgagagcg gcccgccaat atcgggccgg
    14941 cgcgcgaacg aaccgggatt ggttccctcg ctacgcgaag atgggttgta ggctccttgg
    15001 agcggcagcg ttctcatttc gaatctcctc tggaattgcg agggaaacgc cgacaccgtt
    15061 tcgcttccct acgccggtgc gaaccggatc aggttctacg ggtgcttctc agcggctatg
    15121 ggccgcgccc cgagcgcgca gcgaccctac ctcaggccgt gcgccagggc aaccggagcc
    15181 gccgcccccg acgccggctt tcgcgccccg aacccggccg tcaccaagtg tcgacaacct
    15241 gcttcgacaa tcctgtcgca tgctagagct caacccgcga tggccacgtc ctcacgcatc
    15301 ttcacgctcg atccgccgcc ggagcctgac tccgacctcc cctcgcgcgc cgacaagaag
    15361 cgtgaggttc gcgagcggga agaacgcctc atgagcctgg ctcgccaaat cgtcgagctg
    15421 agcccgcggc tcacgaccaa gctcgagttg ccggacctgc tgctcgaagc cgtcgaccat
    15481 gcgcgtcgga ttccgagcgc gcccgcgcag tatcgcgccc tccgcaaagt gcgcacgctc
    15541 ctgcgggacg acgacgtgcc cgagctcgag cgtcgcctcg ccgatctgct cgacccttcg
    15601 cgcgtcaacc ccagcaccga ggccgtcgcc cactggctcg cccggctcat cggcgaaggc
    15661 gagcccgcgc tcgacgccat cgtggagcaa tacccgaacg ccgatcgccg ccagctccgg
    15721 caactctcgc gcaacgtcac ccgtgcgagt gaagcgaacc gtgccgccgc cgaacaagcg
    15781 ctgaagaagg tgctgcgcga tgtcatccga ggcgcccccg gcgcgacgtg atggcaacat
    15841 gaaatgttcg ctagttgcat tgctgttgct tgcgagctgc ttttctccgc gtttgagccc
    15901 acccgcctcg gaccgcgcgc cgcgcaagta cgagcgcacg gacagcacat ccaaagtgct
    15961 cgaagtggag agctacgttt ggacttcgac ggacgctgcg acctgtgaga caccgcgtgc
    16021 ccccccctcc gcggcaagat tcgcagctct tccagctgaa tcttcggcaa gttccgcgtc
    16081 gccagcttcc ggggtagaca cgacgaattc agcaaccccg gggtcgattg cggacgcggc
    16141 caaaaccgtg gaaggaatgc ggcccggatt caatgcctgt tacaaagatt ttctgcgcag
    16201 gagcgggcgg catgggcatt cgggggcaat ccgcctccgg ctcgacgtga attgcgttgg
    16261 cgcagtctcg aggctgcgcg ccgacgccta cggcttggat cgccgcgcga t
//
LOCUS       DASTKU010000149         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_249388, whole genome shotgun sequence.
ACCESSION   DASTKU010000149 DASTKU010000000
VERSION     DASTKU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249388"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            772..1368
                     /locus_tag="VFQ61_05410"
     CDS             772..1368
                     /locus_tag="VFQ61_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953915.1"
                     /translation="MRVLPEASPRALQVSWMQHRLAFHVLTAGLVLIAGGGCRTDKSA
                     GERTAIALRDFAVTLELPASWAVVAGQSDTTEGLVAFSGPCGSGYSGSGFLDASSQRA
                     PASIEEAEREALKADGCREAGACNALAREPLPDGGYLVTTRSSRAVRVESWVHLGPNQ
                     VVRCGAEVFREGAAPESPKQAAGCRAWIEKMCRSVTLK"
     gene            1424..2020
                     /locus_tag="VFQ61_05415"
     CDS             1424..2020
                     /locus_tag="VFQ61_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953916.1"
                     /translation="MSTAHWALVILSGVVTLGTGIAAWLTVLPALKARAMLRLSSTPS
                     LDRREIPAPREPQTGLVRGVRGALLEARLALLSAETLLKDALRASRLDLLGLAFDRGR
                     AKQSSLGAAARHMRNAQQRIADARRHSANPTSLENIEFDCDPLLSELETSWLGDGLFL
                     DWMVHRRILKALDQVARMLEGTEALIRELDESTASVGE"
BASE COUNT          444 a          830 c          814 g          441 t
ORIGIN      
        1 cagcgcggcc gcgccagcgg tcagcgacgc cagacccctg cccaacccaa ccctcgagac
       61 cgcaccgaat aaagtcatgc caaaactccc accgtaagtc gctttcgtgt accgcgaagc
      121 cgctgcagag gccagtgcca cgaagtgaat gacgccgcat caaagagcgc gaccttcaaa
      181 acagatcgct ctctccatct gaacgcgctg acggggcgga cctcgcgtgc ctggcggctc
      241 aactgctgcg cgtcgccctg aagacgttca gtgacggcac cgtcaccgtc gacctcgacc
      301 cgctgtcgct gctctgccgc ctggtcgcac tcgtgccggc gcccaggctc cacacagtcc
      361 gccacttcga cgagtgatcc agtacggcgt ttggtccgaa acagcggtgc ccgcgtgccg
      421 ctctcgcgcc actatgcgcc cacccgccag ccgggaagaa cggggttcat gcaccggata
      481 cgcaggtcct gctcaccaat ctgacgatta cgggcgttga cccaatcctc gagatcttta
      541 gagggggcaa cgtgtgagtt gcagcaggcc ggcgcgggtg caggccctgg gtacttgcta
      601 gcgcgcgtca gcgttcgcgg tcaggtctgg tcccgcaact ccgaaggaag cagaccttct
      661 cgatgagaac gctcgtgtcg aacgtgaacg tgagtgggaa agacctgcag ctcggcgtga
      721 gcgcttccgt ggtgggcggc gcgctgattc gagtcggctg agggggcgct ggtgcgagtt
      781 ctgccagaag cttcgccgcg agcgctgcag gtgagctgga tgcagcatcg gcttgcgttt
      841 cacgtcctaa ccgcaggctt ggttctcatc gccggcgggg gttgccgaac cgacaagagc
      901 gccggcgagc ggacggcgat cgcgttgcgc gattttgcgg tgacgctcga gctgccagct
      961 tcttgggcag tggtggcggg gcaatcggat acgaccgagg ggctcgtcgc gttctcgggt
     1021 ccctgtggtt ctggctactc gggcagtggc ttcttggatg catcttcgca gagggctccc
     1081 gcctcgatcg aagaagcaga gcgcgaggct ttgaaggcag acgggtgcag agaggccggg
     1141 gcgtgcaacg cgctagcgcg agagcctctc ccggacggcg ggtatctcgt gacgactcgc
     1201 tcgtcgaggg cagtccgagt agagtcttgg gttcatctcg ggccgaacca ggtcgtgcgc
     1261 tgcggcgcgg aagtgtttcg agagggcgcc gcaccggaat caccgaagca agccgcgggg
     1321 tgccgtgcat ggatcgagaa aatgtgccgc tcggtgaccc tcaagtaggc gggggagaac
     1381 ccgtgcacct ggagctcgac acgcttcgag tcataatctg ctcgtgagta ccgcgcattg
     1441 ggcgctcgtc atcctgtccg gcgtcgtgac gttgggcacg ggcatcgccg cgtggctgac
     1501 ggtgctgcca gcactcaagg cgcgcgccat gcttcggctc tcttcaaccc cgtcgctcga
     1561 ccgtcgggag attcctgcgc cccgggaacc acagacgggc ctagttagag gcgtcagagg
     1621 cgcgctcctc gaagcccgcc ttgcgctcct ctcggcggag acgctcttga aggatgcgct
     1681 gcgggcgtct cgtttggatt tgctcgggct tgctttcgac cgcggtcgcg caaagcaaag
     1741 ctccttgggc gcggcggcga ggcacatgcg caacgcgcaa cagcggatcg cggacgcgcg
     1801 gcgccactcg gccaatccca cgtcactcga gaacatcgag ttcgactgtg acccgctgct
     1861 cagtgagctc gagacatcct ggttgggaga tggtctcttc ctcgattgga tggtgcaccg
     1921 ccgcatccta aaggctctgg atcaggtcgc gcgtatgctc gaaggcacgg aggcactgat
     1981 tcgcgagctc gacgaatcga cagcatcggt tggggagtga tcccgtacgg gagtgtggga
     2041 gcgatcccgt acgacgttac gtctgagcga tcccgtacga cgttacgtct gacggcgagc
     2101 gatcccgtac gacgttacgt ctgcgagcga gcgagcgatc ccttacgacg ttacgtctgc
     2161 acccagcgcg gttcggctcc tcaaaacggc cggcttcgca ctcgcaccgg tcgccagaag
     2221 cgccaaatac gcctcgtgag aagcacccaa tacgcctcca gagggtcctc cccgcagacg
     2281 cacctctccc ctcccgcatg gccgcagtcc gtaagccgca cttccgcccg cggccgcgtg
     2341 gcacgcgacc caaagcacgc tccgcgctgc tagcctaacg gcaccgacgc acagcgatgg
     2401 cccatcagac gcattcgata cgtcccatac gggaacatcg cttccagctc gccccccgct
     2461 actcgctgcc gcgcaacttc gtgcgcccga cggaatgcga ccagctgctc aagcacgcgt
     2521 acacggctc
//
LOCUS       DASTKU010000150         6559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1366064, whole genome shotgun sequence.
ACCESSION   DASTKU010000150 DASTKU010000000
VERSION     DASTKU010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366064"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /gene="mraY"
                     /locus_tag="VFQ61_05420"
     CDS             <1..448
                     /gene="mraY"
                     /locus_tag="VFQ61_05420"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET9953917.1"
                     /translation="YIAGSVLFGRPLSEYLDVAGFPEMAELAVYAAAMIGSGIGFLWY
                     NTYPAQVFMGDVGSLALGGGLGMLAVLTKTELLSVILGGVFFVEAVSVIAQVGSFKLF
                     KKRVFLMAPIHHHFEKKGWPEPRIIVRFWIISVLLALFALSSLKLR"
     gene            484..1812
                     /gene="murD"
                     /locus_tag="VFQ61_05425"
     CDS             484..1812
                     /gene="murD"
                     /locus_tag="VFQ61_05425"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET9953918.1"
                     /translation="MLQGRKVIVVGLGKSGVSAAKLCLAFGAQVVGTDAAPRENLSQA
                     ARELGIELFTSGHEAVDFESFDLIVVSPGVPMLPALERAQRAGVEIVGELELAARYLT
                     APLLLVGGTNGKSTTTTLLGDLIRTSGKRVFVGGNLGTPACEAVSGAYDYVVFEVSSF
                     QLEHAPQIKPRVNILLNVTEDHLDRYPSFQAYADAKGNSFVNQSAEDVAIIPSGDAQC
                     EQQARRGGGRIVYFGGADYVVSASSILERATGERFDLSDSRLFGTHNHLNAAAAVAAA
                     RAVSCASSAVRQALQAFEPLAHRMARVARHHDVNYYDDSKGTNVGASVTALLGLSEER
                     AVLIAGGRDKHGSYEPLVEALLRKGRAVVLIGEASARIAEAIAGRVPTVQAESMIEAV
                     RKARELARPGDAVLLSPACSSFDMFKSYADRGDQFARAVQTMITQNGGAP"
     gene            1809..3077
                     /gene="ftsW"
                     /locus_tag="VFQ61_05430"
     CDS             1809..3077
                     /gene="ftsW"
                     /locus_tag="VFQ61_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HET9953919.1"
                     /translation="MSAGALTGGVTGLKEVTGLKEQRGPMDVVLAAIVIGLLGFGVVM
                     VYSASVIEATVVFRDPQYFLKRQTVFVAASLFVMFVVSRFDYRKLKRFTYPLLMAVTG
                     LLVLSVIGFGHTGGGAARWLALGPIHVQPSEAAKLALVLWLAYSLEKKRERVRSFSIG
                     MLPHLIMAGLLMVLCLKQPDFGGATVLLLLTFTLLFVAGARLGYLLGATLLGVLFAAW
                     AVRFTPYRWERMLAWFNMADHRQDLAYQPFQAVMSFGSGRVFGMGLGKGLQVLYLPEA
                     HTDFISAIVGEELGFVGILALGAAYLVVVARGVRVAMAAPDEYGSYVAFGISVLFGVQ
                     ALVNMAVAMSMLPTKGLTLPFVSYGGSSLLVSSAAMGVLLNVSRRSSLTGNRKLGKAG
                     DPRPEASALLVSEAAEPVPSRSRAVKEATA"
     gene            3074..4180
                     /gene="murG"
                     /locus_tag="VFQ61_05435"
     CDS             3074..4180
                     /gene="murG"
                     /locus_tag="VFQ61_05435"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET9953920.1"
                     /translation="MSEPCILFAGGGSGGHVFPMLAVAEELTRLEPHARVIFVGTARG
                     QEARLVPERGYTLELVEVSPIRGGGLTGAMRGVRSAALSLPSAVSLVRKHRPKLVFSI
                     GGYAAGPVALAARSLGVPLAVLEPNAEIGLSNRLIAPLAQRAYIAFPRTRRWFSSSVV
                     RETGVPIRSGFEPAEFPAGSPQVLVLGGSQGAKSLNERVPQALARLQSRPQVIHQCGS
                     AHAEATRELYASVGLSERVRVLPFIADMPRALAEAQLVIGRAGAGAVSEICAVGRGSL
                     LIPYPFAGDHQRFNTQAMVECGASVVLSDVDATPERLSQELEGLLRSPLRLQNMARAA
                     RGLGRPHAAAEIARDLLSLAGSSTASSQPSGEVV"
     gene            4180..5592
                     /gene="murC"
                     /locus_tag="VFQ61_05440"
     CDS             4180..5592
                     /gene="murC"
                     /locus_tag="VFQ61_05440"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET9953921.1"
                     /translation="MFRGRVRHVHFIGVGGIGMSGLAEILRSLGFDVSGSDVKPGENT
                     ERLLRLGVRIDIGHEAKNVIGSDVVVYSSAIQPNNPEITQARELGIPVIARAEMLAEL
                     MRVKYGIAIAGSHGKTTTTSLVATVLRAAGLDPTVVVGGRMAALASNARLGSGDLLVA
                     EADESDGSFLRLQPTIAVVTNIDAEHLDFYHTHAAVKDAFVAFIERVPFYGLSVLCLD
                     HPHVQDILPRVGRRHVTYGLSPQATYFARNIVFSGLMTSFDVFRRGEALGRFSVRMPG
                     QHNVLNTLAVIAVADELEVPLDVVKQSLATFHGVARRFTVVEEVDGIALVDDYGHHPA
                     EIEATLAAARKAYSERVLVAFQPHRYTRTQLLFEEFTRAFNQADELIVTDVYAAGEAP
                     IPGIDSAALVQGIRGHGHARARYVADKSQLAGELARLARPGDVVIALGAGDINKVLPL
                     VARELRNSRAPTEQAGGQGT"
     gene            5589..6494
                     /locus_tag="VFQ61_05445"
     CDS             5589..6494
                     /locus_tag="VFQ61_05445"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET9953922.1"
                     /translation="MKGDIASARRAQRHGAQVSAPEEVFAVGESPLPGPVEAKALDGS
                     RAPRGRTGRALQALKVGLGALLVVGTSLGVAFSARRYALTSDRFAIQHISVTGGRRFD
                     AARIRQVGKMPPGQNLFALDTQVVEARLLDDPWVASAKVVRRLPSTLEVTITEREAKA
                     LALLGDRLYLVTPNAEPFKQVEGEDPVDLPVITGLSPESLLRDRPRALERLALGLELL
                     AQYQELNLSRAYVAEEVHLDESGTVTLIVGKDGIVLNLGRPPYRPRLLMAERVVEEAR
                     RSGHLPGIVFADNEAHPERVVVRMR"
BASE COUNT         1042 a         2044 c         2206 g         1267 t
ORIGIN      
        1 ctacatcgcg ggctccgtgt tgttcgggcg cccgctgtcc gagtacctcg acgtagcggg
       61 cttcccagaa atggcggagc tggccgtcta cgcggcggcc atgatcggct ctggcatcgg
      121 ctttctttgg tacaacacct atcccgcgca ggtcttcatg ggtgacgtgg gatcgctagc
      181 gctcgggggc gggctcggca tgttggccgt gctcaccaag accgagctgt tgagcgtcat
      241 cctagggggc gtgtttttcg tcgaggcggt cagcgtcatc gcgcaggtcg gctctttcaa
      301 gttgttcaag aagcgcgtct ttctgatggc gcccatccac caccacttcg agaagaaggg
      361 gtggccggag ccgcgcatca tcgtgcgctt ctggatcatt tccgtgttgc tcgcgctgtt
      421 cgcgttgtca tcgctcaagc tgcgctgagc cgcagcccgg cgcgagacga ggtgaggtag
      481 aacatgcttc agggtcgcaa agtcatcgta gttgggctcg gcaagagcgg cgtcagcgcg
      541 gccaagttgt gtctggcgtt cggcgcgcaa gtcgtgggta cggacgccgc gccgcgcgag
      601 aacctgtccc aagccgctcg cgagttgggc atcgagctgt tcacctccgg gcacgaggcg
      661 gtcgacttcg agagcttcga tctaatcgtg gtgtcgcccg gggtgccgat gctgcccgcc
      721 ttggaacgcg cccaacgcgc gggcgtcgag atcgtcgggg agctcgagct cgcggcgcgc
      781 tacttgaccg ccccgctgct gctcgtgggc ggcacgaacg gtaagagcac gacgacgacg
      841 ctgctcggag atttgattcg gaccagcggc aagcgggtgt tcgtgggtgg aaacctgggc
      901 acgcccgcct gcgaggccgt gtcgggagct tatgactacg tggtgttcga ggtctcgagt
      961 tttcagctcg aacacgcgcc gcagatcaag ccgcgggtca acatcctgtt gaacgtaacc
     1021 gaggatcacc tcgatcgtta ccccagcttc caggcttacg ccgacgccaa gggcaactcg
     1081 ttcgtcaatc aaagcgccga agacgttgcg attatcccga gcggggacgc gcagtgcgag
     1141 cagcaggcgc gacgcggcgg gggccgcatc gtctacttcg ggggcgcgga ctacgtcgtg
     1201 tcggcctcca gcattttgga gcgcgcgacc ggcgaacgct tcgacttgag cgacagccgg
     1261 ctattcggga cccataacca cttgaacgcc gcggccgcgg tcgccgccgc tcgcgcggta
     1321 tcctgcgcgt cgagcgccgt gcgtcaggcg ctgcaggctt tcgaaccgct ggcgcaccgc
     1381 atggcccggg tggctcgcca tcacgacgtc aactactacg acgactccaa gggcaccaac
     1441 gtcggagcct cggttacggc gctactcggc ttatccgaag agcgtgccgt cctgatcgcg
     1501 ggcggccgtg acaagcacgg ttcttacgag ccgttggtgg aggctctttt gcgcaaaggt
     1561 cgagccgtcg tgctgatcgg tgaagccagc gcccgcatcg cggaagcgat cgccggccgc
     1621 gtgccaacgg tgcaggccga gtcgatgatc gaggccgtgc gcaaagcgcg tgagctagcc
     1681 cgaccaggag atgcggtgtt gctcagcccc gcgtgttcta gcttcgacat gttcaagagc
     1741 tacgccgatc gcggggacca atttgctcgc gcggtccaga ccatgatcac ccaaaacgga
     1801 ggcgcgccat gagcgcggga gcgctgaccg gcggcgtgac ggggctgaag gaagtcaccg
     1861 ggctcaaaga acagcgcggc ccgatggacg tcgtgctcgc ggcgatcgtg atcggtctgc
     1921 tcggcttcgg agtcgtgatg gtctactccg cgagcgtcat cgaagccacg gtggtgtttc
     1981 gcgatccgca atacttcctc aagcggcaga cggtgttcgt tgccgccagc ttgttcgtga
     2041 tgttcgtcgt ctcgcgcttc gactaccgga agctgaagcg tttcacctat ccgttgttga
     2101 tggcggtcac cgggctcctc gtgctcagcg tgattggctt cgggcacacc gggggcggtg
     2161 ccgcgcgttg gctcgctctc ggccctatcc acgtgcaacc ctcggaggcg gccaagctcg
     2221 cgttggtgct ttggttggcc tactcgctcg agaagaagcg cgaacgggtt cgaagcttct
     2281 cgattggcat gcttccgcac ctgatcatgg cggggttgct gatggtcttg tgcttgaagc
     2341 agccggactt cggcggcgcc acggtgctcc tgctcctgac cttcacgctg ctattcgtcg
     2401 ccggcgcgcg gctcgggtac ttgttggggg cgaccttgct gggagtcttg ttcgcggcct
     2461 gggcggttcg cttcacgccc tatcgctggg aacgcatgct ggcgtggttc aacatggcgg
     2521 atcaccgcca ggatctagcc tatcagccat ttcaggccgt gatgagcttc ggttcggggc
     2581 gggtattcgg gatgggcctc ggcaaaggtt tgcaggttct gtatttgccc gaagcgcaca
     2641 ccgacttcat ctccgccatc gtcggcgaag agctcgggtt cgtaggcatc ctcgccttgg
     2701 gcgccgcgta cttggtcgtg gtggcacgag gggtgcgcgt cgcgatggcc gcgccggacg
     2761 agtacggctc ctacgtcgcc ttcggcatct cggtgctttt cggagtgcaa gcattggtga
     2821 acatggcggt cgcgatgtcc atgctaccaa ccaaggggtt gaccctgccg ttcgtgagct
     2881 acggcgggtc gtcgctcttg gtgtcatcgg cggccatggg cgttctcttg aacgtatcgc
     2941 gccgctcttc gctgaccggc aatcgaaagc tcggcaaagc cggcgatccc cgtcccgagg
     3001 cgagcgcgct tctggtgagc gaggccgcgg aacccgttcc ctcccgctct cgcgcggtca
     3061 aggaggccac cgcgtgagcg agccctgtat actattcgct gggggcggta gcggcggcca
     3121 cgtctttccg atgttggctg tcgccgagga actcacgcgc ctcgagcccc acgcgcgcgt
     3181 aattttcgta ggcaccgcgc gcggtcaaga ggcgcgcttg gtccccgaac gcggctacac
     3241 tttggaactc gtcgaggtct ctcccatccg tggtggcggt ctcacgggag cgatgcgcgg
     3301 cgtacggagc gcggccctga gcttaccgtc cgccgtcagt ctggttcgta agcatcgacc
     3361 caagttggtg ttttcgattg gaggctacgc ggcgggtccg gtggcgctcg cggcgcggag
     3421 cctcggtgtg ccgttggcgg tactcgagcc gaacgccgag attggcctga gcaaccgtct
     3481 gatcgcgccg ctcgcacagc gcgcgtatat cgcgttcccc cgcacccggc gttggttctc
     3541 ttcgtccgta gttcgtgaga ccggcgtacc gattcggtca ggcttcgagc ccgccgagtt
     3601 cccggctggc tcgccccagg tgctcgtgct cggtggaagc caaggggcca agagtttgaa
     3661 cgaacgtgtt ccgcaagctc tcgcgcggct ccagagccgc ccgcaagtca ttcaccaatg
     3721 tggctccgcg catgccgagg ccacgcgcga actctacgca agcgtgggct tgagcgagcg
     3781 agttcgcgta ttgcccttca ttgccgacat gccgcgagcc ctcgccgagg cccagctggt
     3841 gatcggccgt gccggcgcgg gcgcggtcag cgagatctgc gcggtggggc gcggaagctt
     3901 gctgattccc tatcccttcg ccggggatca tcagcgcttc aacactcaag ccatggtcga
     3961 gtgcggagcg agcgtggtgt tgtcggatgt cgacgctacg cccgaacgcc tgagccagga
     4021 attggagggc ctgctccggt cgccgctgcg cttgcagaac atggcacgcg ccgcgcgtgg
     4081 attggggcgc ccgcacgcgg cggccgagat cgcccgcgac ttgctgtcgc tcgccggctc
     4141 atccacggct tcgagccaac cgtcaggaga ggtagtctga tgtttcgcgg gcgcgtaagg
     4201 cacgttcact tcatcggtgt cggtggcatt ggcatgagtg gtctcgccga gatcctgcgt
     4261 agcctcggct tcgatgtctc gggctcggac gtcaaaccgg gcgagaatac cgagcggctc
     4321 ctgcggcttg gggtgcgcat cgatatcggg cacgaggcga agaacgtcat cggctctgat
     4381 gtcgtggtct actcgagtgc gattcagccc aataacccgg agatcaccca agctcgggag
     4441 ctgggaattc cggtcatcgc gcgggccgaa atgctcgccg agctgatgcg cgtcaagtac
     4501 ggtatcgcga tcgccgggtc tcatggcaag accactacga cctcactcgt cgcgaccgtg
     4561 ctgcgcgccg ctgggttgga ccccaccgtg gtcgtcgggg gacgcatggc cgcgctcgcg
     4621 agcaatgccc ggctcggcag cggagatctg ttggttgcag aggcggacga gagcgatggc
     4681 tcgttcttgc ggctgcagcc gacgatcgcc gtcgtcacga acatcgacgc cgagcatctg
     4741 gatttctacc acacgcacgc ggccgtcaaa gacgcgttcg tggcgttcat cgagcgcgtg
     4801 ccgttttacg gcctttccgt gctgtgcctc gatcaccctc acgtgcaaga catcctgcct
     4861 cgcgtggggc ggcgccatgt gacctacggg ctttctccgc aagccaccta cttcgcgcga
     4921 aatatcgtat ttagcgggtt gatgacgagc ttcgatgtgt ttcggcgcgg cgaagccctc
     4981 ggtcgtttca gcgttaggat gccagggcag cacaacgttc tgaacacgtt ggccgtgatc
     5041 gcggtcgccg acgaactcga agttccgctc gacgtagtca aacagtctct cgccaccttc
     5101 cacggtgtgg ctcggcgctt tacggttgtc gaagaagtgg acgggatcgc gctggtcgac
     5161 gactatggcc atcatccggc ggagatcgaa gccactctgg ccgcagcccg caaagcttac
     5221 agcgaaaggg tcctcgtggc ctttcaaccg caccgctata cccgcactca gctgttgttc
     5281 gaggagttca cgcgcgcctt caaccaggcg gacgagctga tcgtgaccga cgtctatgcc
     5341 gctggcgaag ccccgattcc cgggatcgat agcgccgccc tcgttcaagg cattcgcggt
     5401 cacggtcacg cgcgggcgcg ttacgtggcc gacaagagcc aactggcggg cgagctcgcg
     5461 cgcctggcac gtcccggtga cgtggtgatt gcgctcggcg ccggcgatat caacaaggtc
     5521 ttaccgctcg tggcgcgaga gttgcgaaac agccgagctc cgaccgaaca agcgggaggg
     5581 cagggcacgt gaagggcgat atcgcgagtg cacggcgcgc gcagcgccat ggggcccaag
     5641 tctctgcgcc cgaagaggtc tttgcggtgg gtgagagccc gctgcccggt ccggtggaag
     5701 ccaaggcgct cgacggaagt cgagcgccgc gcggccgaac gggccgtgct ttgcaggccc
     5761 tgaaggttgg attgggggcg ttgttggtgg tcggcacctc gttgggggtc gccttcagcg
     5821 cccgtcgtta tgcgctcact tccgaccggt tcgcgatcca acacatctcc gtgacgggag
     5881 gtcggcgctt cgacgcagcg aggattcggc aggtcggcaa aatgcccccc ggccagaacc
     5941 tgttcgcgct ggacactcag gtggtcgaag cgcgtttgct cgacgatcct tgggtcgctt
     6001 cggccaaggt cgtgcgccgc ctgccttcca ccctggaggt gacgatcacc gagcgcgaag
     6061 ccaaggcctt ggcgctcctc ggggaccgcc tctatctagt cacgcccaac gcggagcctt
     6121 tcaaacaagt agagggcgaa gatcccgtcg atttgccggt aattaccggc ctttcgcccg
     6181 agagtttgct gcgggatagg ccacgggcgc tcgaacggct cgcgctaggg ctcgagctct
     6241 tggcccagta ccaggagctc aacctcagcc gggcgtacgt cgcggaagag gtgcacctcg
     6301 acgagagcgg cactgtcacc ttgatcgtgg gcaaagacgg gatcgtcctg aatttgggcc
     6361 gtcccccgta tcggcccagg ttgctgatgg ccgagcgcgt agtggaggag gctcggcgct
     6421 cgggccacct gcccggcatc gtgttcgcgg acaacgaggc ccaccccgaa cgggtggtcg
     6481 tgaggatgcg atgacgagtt gccaaaactt ggccacttct agaagcacat ggcagcttgc
     6541 cgcgagcgta gagcagcgc
//
LOCUS       DASTKU010000151         5325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1589505, whole genome shotgun sequence.
ACCESSION   DASTKU010000151 DASTKU010000000
VERSION     DASTKU010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589505"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFQ61_05450"
     CDS             <1..550
                     /locus_tag="VFQ61_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9953923.1"
                     /translation="GRMRFAPREYDLAEVVRNAVARQPAFDDGRLRLVADLPKRGALR
                     GTGDVDRLGRALDQLFDNAAKFVPAGGTVGVRARRLPSGHYEICIADTGPGIPQEKAR
                     RLFEPFYQVDGSPTRLHGGTGVGLAIVRGVAQGHGGDVRLASPADEEIVGVKFQGAAF
                     YVVIASDTSTGPVSELPSRPFS"
     gene            560..1732
                     /locus_tag="VFQ61_05455"
     CDS             560..1732
                     /locus_tag="VFQ61_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET9953924.1"
                     /translation="MVSKHEAENSDGLTGAIYLDWNATTPPHPDVLKAMLAAATTAWG
                     NPASVHGSGRRARVLVEELREELASALQVHPRDVVLTSGGTEANNLGLAHASGLVTSR
                     LEHASIVRVAEAMEAAGKPVRWLPVATTGQIDPAAVALACEGLSEGSWIAIQAANHET
                     GALQPIEAVSEVARRARMKLHVDAVQAFGKVDVRPWLPLADRIAIAAHKIRGPKGIGA
                     LAFRGKAPGPVLRGGSQERGLRPGTVDPIACAGFRRAVQLASDGPARYASLAQRRDEL
                     EAAFRGLARVNAADSPRLPHVSNLSFAGVSGDELVAALDLRGIQISSGSACAAGTAEP
                     SPVIVAMHGRELARSAVRISFGDTTPPSALDRLKEAIFQLLDAEGASPGAQPSSAS"
     gene            complement(1929..2738)
                     /locus_tag="VFQ61_05460"
     CDS             complement(1929..2738)
                     /locus_tag="VFQ61_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET9953925.1"
                     /translation="MKSTRYSARHRPEPRQPTIDSPDPSAPRLVLGLQSVRAAIRRHG
                     RALAAVWLESDDNPRLQAVQRFASDHGVPRIEVVGRDQLARLSGGVLHQGVAAFAPEL
                     AFQAFEPLLEEPSLLGIALDRIQDPQNFGAVVRSAVGLNASCVIWGEHSSAPLGTTTA
                     RASAGAVEFARLCRVPSLPRALTDAAQAGIQIVGLDAQAKSALHEVDLRRPSVLVIGS
                     EHDGMTPAVRRACTTLARLAGPSAVESLNASVAAAIALYECSRQRTGQSSV"
     gene            complement(2701..3438)
                     /gene="pyrF"
                     /locus_tag="VFQ61_05465"
     CDS             complement(2701..3438)
                     /gene="pyrF"
                     /locus_tag="VFQ61_05465"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET9953926.1"
                     /translation="MSSELTDARSRLIYALDYPTLPAAERGAREVAPSVGWLKVGLEL
                     FVHEGPRAVALGSQLGCRVFLDLKLHDIPETVERAVAVAGQLGAGLLTIHASGGPRML
                     EAAAQRAQRENTGLRLLAVTVLTSLDAKDLGAVGVSDEPGTQALRLARLAVGCGVSGL
                     VCAVTDAPALRRELGPVPWLVTPGIRPAGSGAKDDQKRVGTPAQAVAAGASLLVVGRP
                     IRDAAQPGQAAREILDEIDQVLRASPT"
     gene            complement(3490..4257)
                     /locus_tag="VFQ61_05470"
     CDS             complement(3490..4257)
                     /locus_tag="VFQ61_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953927.1"
                     /translation="MAEQKESSVLFSLKELMSLEENRIKEEEAEKERRVRAEAEARAA
                     AERAQREAEERRIREEEERRRAEEQRQREEAARLEAIRLAEIEKAKAEAEHRARMEAM
                     AAQQSHEQQLAALHSDQTKKRLKMIVGIGAAVVVAALVGIILIVKSSNEERRRAEAQA
                     AAQQQSAAAELARLKAEADAFARKEEELRRQLDTVKDQATRDRLEAELAKAQQETTRA
                     RGRAPGANAGSSPASKPGGGASKPCTCDRSDPLCSCL"
     gene            4949..5173
                     /locus_tag="VFQ61_05475"
     CDS             4949..5173
                     /locus_tag="VFQ61_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding carrier protein"
                     /protein_id="HET9953928.1"
                     /translation="MALMPTEVLAHITGTVWKIEVKVGDQVSAGQTLVILESMKMEMP
                     IEAGEAGNVSEILTHEGAAVDEGDVLLRLT"
BASE COUNT          921 a         1662 c         1723 g         1019 t
ORIGIN      
        1 cggccgaatg cgcttcgccc cacgcgagta cgatttggca gaggtcgtgc gaaatgcggt
       61 cgcgcggcag cccgctttcg acgacgggcg tttgcgtctg gttgctgact tgccgaagcg
      121 tggggctctg cgcggcaccg gtgatgtgga tcgactcgga cgcgcgctgg accaactttt
      181 cgataacgcc gccaagttcg tgccggcggg cggaacggtt ggcgtgcgtg ctcgccggtt
      241 gccttctggt cactacgaaa tctgcatcgc cgacacgggg cccggaattc cgcaggagaa
      301 ggctcgccgg ctcttcgagc cgttctatca agtggacggc tcgccgacgc ggctccatgg
      361 gggcaccggg gtagggttgg ccatcgtgcg aggggtagcc cagggtcatg ggggtgacgt
      421 gcgactagcg tcaccggccg acgaagagat cgtcggagtg aagtttcaag gtgccgcctt
      481 ctacgtcgtt attgcgtcgg atacatcgac agggccagta agtgagctgc cctctcgtcc
      541 ttttagttga gccgcgttcg tggtttcgaa gcacgaagca gagaactccg acgggctgac
      601 gggcgccatt tatctcgact ggaacgcgac gacgccaccc caccccgacg tgttgaaggc
      661 gatgctggcg gcggctacta cggcgtgggg caaccctgcc agcgtgcacg gttcgggtcg
      721 acgcgcgcgc gtcttggtcg aggaactccg ggaggagctc gcgtcagccc ttcaagtgca
      781 tccgcgtgac gtcgtgctca cctcgggcgg gacagaagcc aataatctcg gtttggctca
      841 tgcgtccgga ttggtgacga gccgtctcga acacgcttca atcgtgcgcg ttgcggaggc
      901 catggaagct gcggggaagc ctgtgcgttg gctcccggtg gcaacgactg gccagatcga
      961 cccggccgca gtggcgctgg cctgcgaggg gctctccgag ggcagctgga tcgccattca
     1021 ggccgcgaat cacgaaaccg gagcccttca gcctatcgaa gccgttagtg aagtggcgcg
     1081 tcgggcacga atgaagcttc acgtggacgc ggtgcaggca ttcgggaaag tcgacgtgcg
     1141 cccgtggctc ccgttagccg accggatcgc aatcgcggcc cataaaatcc ggggacccaa
     1201 aggcatcggc gctctggcct ttcggggaaa agccccgggc cccgtgttgc gtggcggaag
     1261 ccaggaacgt gggttgcgcc cgggtacggt ggatccgatc gcttgcgccg gttttcggcg
     1321 agccgtgcag ctagcctccg atggtcccgc acgttacgcg agcttggcgc agcgtagaga
     1381 tgaactggag gccgcgtttc gaggcctcgc gcgcgtgaat gccgccgaca gccctcgctt
     1441 gcctcacgtc tcgaatctgt cgttcgccgg ggtatctggc gatgagctgg ttgcggcact
     1501 ggacctgcgc ggaattcaga tctcgagcgg cagcgcgtgc gcggcaggaa cggccgagcc
     1561 ctccccggtg attgttgcga tgcacggccg cgagctggct cgcagcgcgg tgcggatcag
     1621 tttcggggac accacgccac cttcggcctt ggatcgctta aaagaagcga tttttcagct
     1681 acttgacgca gagggagcat cgcctggtgc gcaaccttcg agtgcttcct gatcctctac
     1741 gcatcttgtg cagacgctgc ctctgagcac gtctgcaagt tactaaaaat aggagctttt
     1801 ccgccgaggc cactccgctc gccggatgat ctactgagta gaatcagccg cgctttgcac
     1861 agagcgcgtg ttcagcgtcg agagctcttg taagtcaagc tttcaatctt gctcgtaaga
     1921 gctcgaattt aaactgaact ttgtccagtc ctctgacgcg agcactcgta gagggcgatc
     1981 gccgcggcca cggatgcgtt caaggactcc acggccgacg gacccgcaag gcgcgccaga
     2041 gtcgtgcacg cgcggcgaac cgcgggcgtc attccgtcgt gttcactccc gatcacgagc
     2101 acgctgggcc gcctcaagtc gacctcgtgc aaggcggact tggcttgagc gtccaagccg
     2161 acgatctgga tgccggcctg tgccgcatcg gtcaaagccc tgggcaacga cggcacgcga
     2221 caaagccggg caaactccac agccccggcc gaggcgcgag cggtcgtagt gccgagcggc
     2281 gcggacgagt gctcgcccca aatcacgcag ctcgcattga ggcccaccgc gcttcggacg
     2341 acggccccga aattctgcgg atcctgaatg cgatccagag caattccgag cagcgacggc
     2401 tcttccagca gtggttcgaa cgcctgaaac gcgagctcgg gcgcgaacgc ggccacacct
     2461 tggtgcaaga cccccccgct caagcgagcc agctgatcgc ggccaaccac ctcgattcga
     2521 ggcaccccgt gatccgatgc gaagcgctgc acggcttgca gccgcgggtt atcgtccgat
     2581 tctaaccaga ccgccgccaa ggctcggccg tgccggcgaa tggcggcgcg aacgctttgc
     2641 agcccgagaa cgagtcgagg tgcgctcgga tcgggcgaat cgattgtcgg ttggcgaggt
     2701 tcaggtcggt gacgcgcgga gtacctggtc gatttcatcg agaatctcgc gggcagcctg
     2761 gcccggctgc gcagcatcgc gaatgggtct gcccactacc aacaaactgg cgcccgcggc
     2821 caccgcttgc gcgggggtgc ctacgcgctt ctggtcgtct ttcgcccctg agccggcggg
     2881 gcgaattccc ggcgtaacga gccaaggaac tggaccgagc tctcgacgca gcgcaggtgc
     2941 atccgtcact gcgcaaacta ggcctgaaac tccgcagccc actgccaggc gagccaggcg
     3001 cagagcttga gttccaggtt cgtccgagac gcccactgcg cccaaatctt tggcgtcgag
     3061 agacgtgagc accgtcaccg caagcagccg caacccagtg ttctcgcgct gggcgcgttg
     3121 cgcagcagct tcgagcatgc gaggcccgcc gctcgcgtgg atggtcagga gccctgctcc
     3181 gagctggccg gcgactgcca ccgcacgctc gaccgtttcc gggatgtcat ggagcttgag
     3241 atcgaggaaa acacgacaac ccagctggct gccgagcgca acggctcggg gcccttcgtg
     3301 aacgaacagc tccaatccca ccttcagcca acccacggac ggagccactt cacgggcccc
     3361 ccgttctgcg gcaggcagcg tagggtagtc gagcgcgtag attagccgcg accgcgcgtc
     3421 cgtaagctca gatgacacgc ggttgcgtcc cccgagaact gcagtgctgg gcagacaaga
     3481 gagctcggct cacaagcagg aacacaaggg atcgctgcga tcgcaggtac atggcttgct
     3541 tgcgccaccg ccgggtttcg acgcagggct cgaccctgca ttagccccag gggcacgacc
     3601 acgcgctctg gtggtttctt gttgcgcctt cgctaactcc gcttccaggc gatcgcgcgt
     3661 cgcctggtcc ttgaccgtat ccagctgacg gcggagctcc tcttccttcc gggcgaacgc
     3721 gtccgcctcg gctttcaatc gtgccaattc ggctgccgca gactgctgtt gggcggcggc
     3781 ttgggcttcc gctcggcgcc tttcctcgtt cgaactcttg acgattagaa tgatgccgac
     3841 gagtgcagcc accacgaccg ccgcaccaat gccgactatc atcttcagac gcttcttcgt
     3901 ctggtccgag tgcagagcgg ccaattgctg ctcgtgcgac tgctgagccg ccatggcttc
     3961 catgcgggcg cggtgctcag cttccgcctt ggccttttcg atctctgcca gccggatcgc
     4021 ctcgaggcgg gctgcctcct cgcgctgacg ttgctcctcg gcgcgccgac gctcttcctc
     4081 ttcacgaatt cggcgttcct ccgcctctct ttgggcacgc tcggctgcgg ctcgcgcctc
     4141 cgcctctgcg cggacacggc gctccttctc cgcttcttct tctttgatgc ggttctcctc
     4201 aaggctcatg agctctttga gtgagaaaag gactgagctt tccttctgtt cggccatggg
     4261 gctgttttcc taggggcgag cccggttttt cctcgggccg gagggttcct cgtgaggaga
     4321 gggtttcgtt tccgcgaaag gcgtcaacga agcttagtgc ccgccgcatc aggcgggtag
     4381 cactcggcaa tcggggcagt caagaagatc ggaagagcgt tcaaggatcc atcggctgac
     4441 tctgccgcgt ttcgtggtct ctggtgggag tcagtctgtt ggaagtcccc ggtgtatcga
     4501 gctggctgac agccgggact gcacgcgaac gcatcagcgg cggccatcgt agtggcatcc
     4561 aactgatcag tcatgtcatt cctatcctgt caatcagatt ggcaagcctt cgggccgact
     4621 gtgacgaaac gcaaccgtag tcgatgttta cccggcttcc cagcatggac acgcttttcg
     4681 ccttcgcgct tgggtaagtg ccgcatccga gtgcgaaact tcggctgtca gtgcgagttc
     4741 gcggctcggg gtagcggggt tgggagtaac cacggttggg agtaaccagg gttttgggta
     4801 accagggttt tgagtaagca cagttgggcg taacacggtc gggcgccgcg cccgcgaccc
     4861 aggccggggt agccccatta gccagtcctg ctttctggac ggcaagcgcg cgcctcgtat
     4921 tgcgacccgc tactgctgtt gaactaagat ggctctcatg cccaccgagg ttctcgcgca
     4981 catcaccggc acggtctgga agatagaggt aaaggtcggc gatcaggtga gcgcaggcca
     5041 aactctggtg attctcgagt ctatgaagat ggagatgccg atcgaggcag gggaggcggg
     5101 caacgtgtcg gagatcctga cgcacgaggg agcagcagtc gacgaaggag acgtgctgct
     5161 tcgcctgacc tgagcctgca ccatcaacct tcggaaaagc tagcgacgag cctcgggctt
     5221 gattggtcca gctggacccg tatcctgagc tgatgcgggt aggatggacc ctgggttgcg
     5281 ttgcgctttc gatcgcttgc ggtggatcgg accacgccgc aggtg
//
LOCUS       DASTKU010000152         3542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_29180,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000152 DASTKU010000000
VERSION     DASTKU010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29180"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFQ61_05480"
     CDS             <1..307
                     /locus_tag="VFQ61_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9953929.1"
                     /translation="AVYIVSQSLKVELPKTANSDEPAQSPLAVTITKEGHYLFEKNQI
                     DEAALVSKLKAAKAGKSDVSLVVTADKEAFHGDVVHVIDLAKTEGILKFAINVERER"
     gene            314..1030
                     /locus_tag="VFQ61_05485"
     CDS             314..1030
                     /locus_tag="VFQ61_05485"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9953930.1"
                     /translation="MAPPKRRRSTVYVVSVGAHLLLGLGLAFIPKQKLREVVGIALAE
                     SKPPEAKPKPPPPVRDEPAKARPARSPNSNARAAARPAVEPASSEPARTEAFTDLGIS
                     LDASAAGGIAVPVAAKPTLNTAPVVPAVVHKPKVLAARLAEPECSEALVKARPERVVQ
                     AEYTDSARAARVEGRVRLELSLNERGEIQDVRVLRGLGYGLDEAALAAARRMRFRPAT
                     RCGKPQGGPFILSMRFLLGA"
     gene            1027..>3542
                     /locus_tag="VFQ61_05490"
     CDS             1027..>3542
                     /locus_tag="VFQ61_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9953931.1"
                     /translation="MKPLRSLFWMASLATLSAPAYAQSGAPTAPEMRPPDARPPDAPS
                     AQLTKPPRLIHYVPPEVKDPPDKPIVILLKIAITAEGAVSDAEVLESADPAYDEPARA
                     AARELVFEPAEIDGRKAPVRIQYRYQIEPPAERPVLPASAEFTGIVRDQHSKQPLSGV
                     RIELDTGEVQTTGDDGRFTFSAVAPGTHSVTLTGPTFTPLGTEETLELGKRYDATYDV
                     EVSAVAITPEDQSDFEVVIVASKLGSKISATEVSAEQGARAAGTGGDVVKVVENLPGV
                     ARSTVGSGQLVVWGASAEDTRVYVDGVHIPVLYHEGGFRSVIHSDLVRSVELQPGGYG
                     ASYGRGLGGLVTVGLKPLDDDRYRGSVQLDIIDTSASLRGPLPKHFNFGVAARRSHLD
                     SVLRAVSSEDVGEFVPIPKYWDGQARLGWSPGDGRSLEVGTLLSSDRIERNLNSADPN
                     EIKRESKTNRFARVYLHYQTQTENSSTRVSPFVGTDRRTLESRFGAVPAEIEVQSTSY
                     GLRANHSVNVAPWLALQAGLDAQVVVSSLGRRGSTTTPPREGDLRVFGQQPADQVNSD
                     DWSVTVASLAPYIESDFSIADKLHIIPGLRIEPQLVRVSRVSPQEGDVPPVGSVREDT
                     GIDPRLSIRWNATNRISAKAAMGVYHQPPLPEDLSSVFGNPNLGFSRATHYLIGGAFR
                     LSKPISLEVTSFYSKSSDLAIRSPLATPRLSQALIPQGQGRAYGTQFLLRYDLIDRFF
                     GWASFSMIRSERSDANGNYRLFDFDQTFVFTALGSYDLGAGFEFGGRFRYSSGYPRTA
                     IESATYDSRLDAYQPVFGAQNDYRIPAFYAFDVRVAKRFE"
BASE COUNT          649 a         1133 c         1101 g          659 t
ORIGIN      
        1 ggcggtttac atcgtctctc agagcttgaa ggtcgagttg cccaagaccg cgaactcgga
       61 cgagcccgct cagtctccgc tcgcggtgac catcaccaaa gagggtcatt atctgttcga
      121 aaagaaccag atcgacgagg cggccttggt ctccaagctc aaagcggcca aggcgggcaa
      181 gtcggatgtg agtttggtgg tgacggcaga caaagaggct tttcacgggg atgtggtgca
      241 cgtgatcgat ctcgccaaga ccgagggcat cctcaagttc gccatcaacg tcgagcgcga
      301 acgctgaagc gtcatggcac cgcccaaacg ccgccgctct accgtctatg tcgtcagcgt
      361 gggagcgcac ctgctgctcg gcttggggtt agcgttcatt cccaagcaga agttgcgaga
      421 ggtcgtgggc attgccctcg ccgagagcaa gccacccgaa gccaaaccca agccgccccc
      481 gcccgtgcgc gatgagccgg ccaaggcacg ccccgcccgc tctcccaact cgaatgctcg
      541 cgccgcggca cgtcccgcgg tggagccggc ctcgagcgag cccgcccgca ccgaagcatt
      601 caccgatctc gggatctcac tcgatgcctc ggcggccggg gggattgccg ttccggtcgc
      661 ggccaaaccc acgctgaaca ccgccccggt cgtgccggcg gtcgtgcata agcccaaggt
      721 gttagcggcg cgcctggcag agcccgaatg cagtgaggct ttggtcaagg ctcgccctga
      781 gcgcgtggtg caggctgaat acactgattc tgcgcgcgcc gcccgcgtag aagggcgtgt
      841 gcgtttggag ctctcgttga atgagcgcgg cgagatccaa gacgtgcgcg tattgcgcgg
      901 tttgggctac ggcctcgacg aagcggcgtt ggcggcggcc cgacgaatgc gatttcgtcc
      961 cgcgacccgc tgcgggaagc cgcaaggcgg accgttcatt ttgtccatgc gttttctact
     1021 cggtgcatga agcctttgag atcgttgttt tggatggcgt ctctagcgac gttgtcggca
     1081 cccgcttatg cgcagtctgg cgcgcccact gctccagaaa tgcggccacc cgatgcacgc
     1141 cccccggatg cgcctagcgc gcagctcacc aagccgccgc gcctcatcca ctacgtgcct
     1201 cccgaggtca aagacccacc ggacaagccg atcgtaatct tgctcaagat cgcgatcacg
     1261 gccgagggtg cggtgagcga cgccgaggtg ttggagagcg cggatccggc gtacgatgaa
     1321 ccggcgcggg cggccgcgcg cgaactggtc ttcgaacccg ccgagatcga tggccgaaag
     1381 gcgccggtcc gcatccaata tcgatatcaa atcgagccgc cggcagagcg cccggttctg
     1441 cccgctagcg ccgagttcac cggcatcgtt cgcgatcaac actcgaaaca acccctctcg
     1501 ggcgttcgca tcgaactcga taccggcgag gtgcagacca ccggcgatga cgggcgattc
     1561 acgttctccg cggtcgctcc cggtacgcac tcggtaacgc taactggccc caccttcacc
     1621 ccgctcggca ccgaagagac cctcgagctc ggcaaaaggt acgatgccac ctacgacgtc
     1681 gaggtgtccg cagtcgcgat tacgccggag gaccaatcgg acttcgaggt cgtgattgtg
     1741 gccagcaaac tcggcagtaa gatttcggcc accgaggtca gcgccgagca aggcgctcgc
     1801 gcggcgggca ccggcggaga cgtggtcaaa gtggtagaga atctacccgg cgtggcccgc
     1861 tcgaccgtgg gttcgggcca actcgtggta tggggcgcca gcgcggaaga cacccgtgtt
     1921 tacgtggatg gcgtgcacat tcccgtgctc tatcacgagg gcggcttccg ttcggtgatc
     1981 cattcggatc tggtgcgcag cgtggagctt caaccgggcg gctatggggc tagctacggt
     2041 cgcggcttgg gcggactcgt cacggttggg ctcaagccgc tcgatgacga tcgctatcga
     2101 ggcagcgtgc agctcgatat catcgatacc tcggcaagcc tgcgcggccc cctgccgaag
     2161 cacttcaact tcggggtcgc cgcgcggcgc agtcacctcg acagtgtttt gcgcgcggtc
     2221 tccagtgaag acgtcggtga gttcgtaccc atccccaagt actgggacgg gcaggctcgt
     2281 ttgggctggt ctccgggtga cggccgctca ctcgaggtcg gcactctgct ctcgtcggat
     2341 cgcatcgagc gtaatttgaa ctcggccgat ccgaacgaga tcaaacgcga gtccaagacc
     2401 aaccgctttg cacgcgtgta cttgcactac caaacgcaga ccgaaaatag cagcacgcgt
     2461 gtgtcgccgt tcgtggggac cgatcgccgc acgctcgaga gtcggtttgg ggctgttcct
     2521 gccgagatcg aggtgcaatc caccagctac gggctgcgcg ccaatcacag cgtcaatgtg
     2581 gcaccgtggc tggcgctgca ggccgggcta gatgcacaag tggtggtgag ttcgcttggc
     2641 cgccgcggct ccacgacgac ccctccgcgg gagggggacc tgcgcgtgtt cggacaacaa
     2701 cccgccgacc aagtcaacag tgacgattgg tcggtcacgg tggcaagcct cgcgccttat
     2761 atcgaatcgg acttctccat cgcggacaag ctacacatca ttcccggtct gcgcatcgag
     2821 ccgcaactcg tgcgcgtcag ccgagtttct ccgcaagagg gggacgtgcc gccggtgggc
     2881 agtgtgcgtg aagacaccgg tatcgatccg cgcctctcga ttcgttggaa cgcgacgaac
     2941 cgcatcagcg ccaaggccgc gatgggggtc tatcatcagc cgcccctgcc tgaagacttg
     3001 agctcggtat ttggcaaccc aaacctcggt ttttcacggg ccacgcacta cctgattggc
     3061 ggggccttcc gcttgtccaa gccgatctca ctcgaggtca ccagctttta ttctaagtcg
     3121 agtgacttgg ccattcgcag cccgctcgcg accccgcgcc tctcgcaagc cctgattccg
     3181 caagggcagg ggcgtgccta cggaacccag ttcttactgc gctatgactt aatcgaccgt
     3241 ttcttcggtt gggcgagctt cagcatgatt cgctctgagc gcagtgacgc gaacggaaat
     3301 tatcgattgt tcgacttcga ccagacattc gtgtttaccg cgctcggatc gtatgacttg
     3361 ggagcaggct tcgaattcgg agggcgcttc cgttactcga gcggataccc tcgcacggcc
     3421 atcgagagcg cgacctacga tagtcggttg gacgcttatc aacccgtgtt cggagcccag
     3481 aacgattacc gcattcccgc cttctacgcg ttcgacgtgc gcgtcgccaa gcgcttcgaa
     3541 tt
//
LOCUS       DASTKU010000153        16654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1367035, whole genome shotgun sequence.
ACCESSION   DASTKU010000153 DASTKU010000000
VERSION     DASTKU010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16654
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367035"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFQ61_05495"
     CDS             <1..534
                     /locus_tag="VFQ61_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953932.1"
                     /translation="GSDNFSFLVPQLPNAVITVAASYGDPGFGEAFSLAHQDVSPGQA
                     NIQLALPAPAVLTAPMASASAVNSNTIFRWVSDDKVFLLHVVDADEYRELFVLTSSKQ
                     IKLPSIAGEFPLRPAADHFWSVQVHGTWPSVDAAAGAEGFQDPFGIWQGDPDGLGRGD
                     GVFSQSEIHDFTTSANP"
     gene            complement(707..1039)
                     /locus_tag="VFQ61_05500"
     CDS             complement(707..1039)
                     /locus_tag="VFQ61_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953933.1"
                     /translation="MEIDGCIGIALVDYETGMCLGASGNPGFDLELAAAGNTEVVRAK
                     KKIRDKLGLRDEIEDILISLTSQYHLIRMVGKNMFFYAVLERQRANLAMARKELHAVE
                     KNLEVDRA"
     gene            complement(1517..5203)
                     /locus_tag="VFQ61_05505"
     CDS             complement(1517..5203)
                     /locus_tag="VFQ61_05505"
                     /inference="COORDINATES: protein motif:HMM:NF015353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HET9953934.1"
                     /translation="MSSRTMRVCSSGRALRGAAGLILSALTNLTAGVGCSSYEGSAPN
                     ADEGLGRTTEALSTTTITEAGDDQRTGHYGNQPRLSPSVVSGSTFGQLFSATVSGQVY
                     AQPLVSKGTLFVVTESNDVYGLDPESGAERWHRNVGTPFNPATLGCNDLTPNIGITGT
                     PVIDSATNVAYFFAKTTQAGVTQWLAHAVDVASGAEKPNFPVLIQGAASNAPGRTFDA
                     IHHLQRPGLLLLGGKVYAAFGGHCDAPDWQGWVVGVSTDGSSQTRWVANTSGNGAGIW
                     QSGGGIMSDGAGRMFVSTGNGEAPVGPILGSQAGSMSGLGEAIVRLDVQTNGSLRAGD
                     FFAPYDAQELDGWDADFASGAPVALPDSFGTTAYPHLLVAVGKQGYVYLLNRDSLGGI
                     GTGPNGGDAYVFRSDANGGVWSKPAVWPGDGGYVYIPTASGGGAAAGSSGFLYAYRRG
                     LGVDGKPTLDFAGRTTDAFGFSSSRPIVTSDGLTAGTGIVWIVWSPGGSGAGSQLRAY
                     RAVPNAKGAFDLLFQAPVGQSAKFNPPGVGDGRVYVGTRDGHVLGFGSPITPVLTATP
                     AQFGNTVVGRDSTVNITLTAAQPLTIQNVLSSSPEFIVGPVASPTLAAGATTTVPITF
                     RPTSSGLKGASVIFTTSAGTASTSLSGKGLSSSAELALSAPALSFGGTTVGGALTQQL
                     VLSNAGATPLTLQGFTSPTSPFSAAQLPAPGTQLAPDASLTLTLQFAPTALGNYASTL
                     GIQTSAGNTSVPLSGNCALPGHLSVTPLSVDYGRVAVSGTRWAKFEVRNTGGVPLTIT
                     KSKPPVLGPFTATTELSEGTVLGVNESLTEWVAYTPPAQGSHDDVWVLNSTGDNGLQE
                     VALHGAAVPGGATSGSVSGWRFNGSAIGSANSFVLTGATEGDQTGSAFWSTALSPAAI
                     DLSFDAILGGGSGADGLALVFADASQQGPTALGGGGGGLGFLGIRGVALALDTYQNGT
                     DPSANFVGLTDGPVNAASQDFNWLATSSAVPALRGDVPVTHHVRVYNAVPGATPSVLV
                     VELDGTEVIRQTVTLPPSVYVGFSGANGGLTDLHAVSNVSIVNQLAPGTGATSVLGFE
                     NPSQWSVSSGTVTASTPTTQGSSALAISRFYYTELVSTPVSNVGPLSGTLAFDFRPPA
                     SLSYGNVQLYVDAPSAGLFMVYVGQVSVAGAQANTYRTLNFAVPSNVSRALGGNYTDL
                     RFKIAVNAPWYSSPYLFDNLRFVR"
     gene            complement(6624..6860)
                     /locus_tag="VFQ61_05510"
     CDS             complement(6624..6860)
                     /locus_tag="VFQ61_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9953935.1"
                     /translation="MKTKAIFYHAGCPVCVQAEQQVASALDPDRYAVEVVHLGKDAAR
                     IAEAERLGVKSVPALVLDEQPFHINFGAALSALR"
     gene            7069..7416
                     /locus_tag="VFQ61_05515"
     CDS             7069..7416
                     /locus_tag="VFQ61_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9953936.1"
                     /translation="MTRRASPRIDSAPYRQLEDVLGCKWSVLVLRAIASGVCRPGALE
                     RHIGGISTKVLGERLRKLEAYKLINKTIYDTVPPRTTYELTAMGQKLIGIIEQIRALD
                     EELQHGMPDTEVE"
     gene            complement(7423..9852)
                     /locus_tag="VFQ61_05520"
     CDS             complement(7423..9852)
                     /locus_tag="VFQ61_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9953937.1"
                     /translation="MLSCLLFTQLARAQSNTPPAESPPESTPPNSAPGGDASEQTKPP
                     ANAVDRDKVESPTTEPETKSAEASAPNAESAIDPSASVESSAGVQPSGVIDSPAEASA
                     APPRETLARPSQDVTIVGTRTAQTAGSAHLINRRQLERFRYTDPQQVLQQAPGVYVRQ
                     EDGIGLRPNIAMRGVDADRSKKLVLMEDGVLFGPAPYSAPAAYYFPLITRMAGVRVIK
                     GPSGVAFGPQTVGGAIDFLTRAIPEQPAGAITISAGQYGFGQLHAHAGATVGKLGFLV
                     EGVRLWNTGFKHLPSGADTGSTRDDWMVKVSYTLESDRYRNEFLIKLAYAEEASNETY
                     LGLTDRDFRADPYRRYPASALDRMENHRTGALATHRFEYPAAHLKLTTSLYRHEYART
                     WRKLNRLEGGLIRDMLLKPDDPRVQDSIAVLRGESDSLAASETLYVGPNQRTFVSQGA
                     QTLLNVDLAQGNFSERVELGVRLHDDRIHRHHSESGYAMRNGELYPVSAPTLVNTVNT
                     ERSTAVALHALAALQYRRLIFTPGLRTELIRMSSEDHRNEGRVTGLVRALVPGAGVYA
                     GLNDDLGLLGGVYRGFSPPPPNGINQNKVEYSVNYEAGARYSRGARRLELIGYFNDYS
                     NLTSVCTFSSGCDDANLDRQTDAGRARTYGFEAFATEELRLSGDWQIPATVAYTWTRG
                     EYLNDFTSEDPTYGQVRKHDEMPYRPRHQLKASVAAEKREVGFISAAATYVSAMRELP
                     GRAPLSQSLVTDEQFLVDVSGGVQLWGPFSMFLNVRNLFDSTFLVSRRPFGARPNPPR
                     WIEIGLRAEAR"
     gene            complement(9881..11323)
                     /locus_tag="VFQ61_05525"
     CDS             complement(9881..11323)
                     /locus_tag="VFQ61_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HET9953938.1"
                     /translation="MTPIESRLRRLAIAAFEPVDIAWLAAFRIAFGLVMAVSMVRFLA
                     YGFVDRFFVSPQFHFKYWGFGWVEPLPPMLMHALFYAMIGLALAIASGAAFRMASALF
                     ALGLAYVQLIDVSTYLNHYYLALLLAGLLTASPAHRIWSVDAWLRPRLGRQRIPCLWL
                     YLFRFQVALVYSFAGLAKAQSDWLLHAQPLRIWLGANTDLPVLGPLFTHPLVPLAMSW
                     CGFLFDSSVVVFLLWSRTRPFAYAVVIGFHVLTRLLFPIGMFPIIMVLSALVFFSPAW
                     PRRLSFHLARYLATYLPPRRAQRARPYDASASTRLAGVSPSQQRRRLLALSLAGGYAF
                     VQLLLPLRFLAYGGNVLWHEQGMRFAWRVMLRAKGGHTTFLVQNKANGRTFYVSPRDY
                     LTGLQESEMSGQPDLILQLAHHIKQDFERRGLGPVEVRAESRVALNGRRGVPFIDPKV
                     DLTSYEDGLLPAQYVLPAPNGAPPHTRPVF"
     gene            complement(11310..12806)
                     /locus_tag="VFQ61_05530"
     CDS             complement(11310..12806)
                     /locus_tag="VFQ61_05530"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HET9953939.1"
                     /translation="MIKRAHNLRLAAAVGCVVLPIGACHETATPREHFGTTAATTGGR
                     ASTPSGGAGAPGSSAGRTTDAGAPHPTSGGTGGHAGTAGTSSTNAASTSTSGGKPDAL
                     PQGGDDSSPVGPGGTSEGGMPSGSGGTTASPVDLCGAPPVEVGEFTREGLRRAAVQCA
                     NWQFCRFEQRSHALREAVEVEGALMTEPTRAEFRRAMLVWGEIELLQFGPLASKSETA
                     GKDVYQGQGIREFIYAWPSTARCRVEEQLASMTFATRGMDGIPISGRGLYALEYLSHY
                     AGNDTNCAAASSAGQAWAGIVDDLPRRKSAYAVAIAADLIAQTQRLRAIYGATSGGFP
                     EAFIGATGYPSEQEALTVLAWALVYLEREVKDWKLGVPAGYTLQHPVTSPETPFAGFA
                     TSVIRANLTGFSRLFQGCGDDGAGLGFDDWLMAAGHAELASDISQALAAARQTADQTP
                     ELDRAQPAQIEALYRAIKQLTDILKTDLFGSGSPLNLKLPAGVEGDTD"
     gene            complement(12810..14624)
                     /locus_tag="VFQ61_05535"
     CDS             complement(12810..14624)
                     /locus_tag="VFQ61_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953940.1"
                     /translation="MRVLRINLVSAALTFCMGASLVVGCGDDDDGPVGQGGTSGGTKA
                     SGGASSKGGGGSGGGSTGGSKASGGSNARGGRTSTSSPEGGADPIGSGGSTEPPQSEG
                     GATSSEGGAVNGGSGGYGGDGEGGSSGGAPEPERAMIAQLKSSVVTQVNDLRGLAFAK
                     SGKLYASGHIGADANVDRQIAVVRFSVDGSLDESFGTGGVLSLNLVPRVTDTTVEPAV
                     VSNDGNEQSLGIVELENGDLIVQANLRDASGKGQDVALLRLDSSGQRVESFGQDGLLR
                     LDFGWTPEDAANWTGTTGPSDDSWGIARDASSGVEKIVVFGAGPAKKGAVTGEPATQR
                     TDNDRYIARVLASTGELDPAFNGGSVFTLNTGGTFADGARRGIVEPDGAIVSSGYTNY
                     GDGLGNHFVVIRLLPNGTPDPNFGFGIGAPGVARGNPFLDDGGIAECYNVTRQSNGRY
                     VTTGYGRATAVNTSSSFGWSTTQSVDLVSFGFMPSGLDRSFGRQGTLAIQSEEYGLGN
                     TEDRGRDVIALADDRLVYVGRFGPNPAIFVTLPDGQLDESVGVGGRLVYPPLGETSQV
                     ATSHFYRVVASPDGKRIAATTNNHPDGVLLALLGVDVP"
     gene            complement(15079..>16654)
                     /locus_tag="VFQ61_05540"
     CDS             complement(15079..>16654)
                     /locus_tag="VFQ61_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953941.1"
                     /translation="ASDTTEILPLDSGFSSRLRTATDALSGRAAQLARQLRVLAAAGA
                     PVRLGYVAEAPVWRASYRLVLDAERDAAMVQGWALIHNDTDEAWARVNIELVNGQPDS
                     FLFPMAAPRYARRQMTTPEERLSTVPQLALKTPDAIWGDHAEEEDDGTGAGLADIESV
                     GGVGYGAGAGGIATAHPMAARAVVSDSISIGDLASVAKAKPVQESLLFSYRLADPISL
                     RAHGSALLPFFDQALDAKRLTRFEAGETQGRATIRLVNASRNTLPAGPLAVFESAGFS
                     GETVLARLRPGGRVFLNYGLDLDTDFREQTRESTRETLQVAFHDMALDEHYVKTRQVT
                     LSFENHSPARRTIAYGLRGTVRNAEISGADALDYDGDTGTALALFEVAPGARSERQLR
                     VREALKDRFSLDELSEEALARMTKAIGLEVSARAALTAAAVVVKSLRAEREREQRLIQ
                     ERSQLDEDLERHREHLKAAGSSDGSTNPLAKRIVELESHRARIRLELEELSRRTTALR
                     RQVAAELQRLPKQEPK"
BASE COUNT         3129 a         5213 c         5211 g         3101 t
ORIGIN      
        1 ggttcggaca acttctcctt cttggtgcct cagctgccca acgccgtaat cacggtcgcg
       61 gccagttacg gagatcccgg ctttggcgag gctttttcgc tagcccacca ggacgtgagt
      121 cccggtcaag cgaacatcca gctcgcgttg ccggctccgg cggtgctcac cgcgcccatg
      181 gcgagcgcga gcgccgtgaa ttccaatacc atttttcgct gggtgagcga tgacaaagta
      241 ttcctgctcc acgtagtcga tgcagacgag tacagagagc tcttcgttct gacgagcagt
      301 aagcagatca agctgccgtc gattgccggc gagtttccgt tgcgcccagc cgcagatcac
      361 ttttggtcgg ttcaggtgca cggcacgtgg cccagcgtcg acgcggccgc gggagcggaa
      421 ggttttcagg atccgtttgg catttggcag ggagatccag atgggctcgg taggggagat
      481 ggcgtgttta gccaatccga gatacacgat ttcaccacct ccgccaatcc gtagaagcaa
      541 ggacgcaata caggtgaact gacttgaacg cactcggatt cgccctggtg ctcgggagat
      601 ccgtgcgttg attcgggcca cctgggagtg cggagtctcg cccgcgctcc cagggaaact
      661 gcacgttcgc gcgaagccga gctcctgctt cgggcgccct ccgatctcac gctcgatcga
      721 cttcgaggtt cttctcgacg gcgtgtaact cttttcgggc catcgcgaga ttcgcgcgct
      781 gtcgctcgag caccgcgtaa aaaaacatgt tctttccgac catacggatc aagtgatact
      841 ggctcgtcaa cgaaatcagg atgtcttcga tttcgtctct caggcctagc ttgtccctaa
      901 tcttcttttt ggcgcggact acttccgtgt ttcctgccgc ggccagttcc aagtcgaaac
      961 cggggttgcc tgaggcgcca aggcacatgc cggtctcgta gtcgaccagg gcgatgccga
     1021 tacaaccgtc gatttccata gccttcctga ttgacgactt aatgtcagac acagtgcatc
     1081 ctccttggat aggttgggtg acggtcacga aatcagggcg ctgcgcgggc cccctcatgg
     1141 ccgcatccct ctgtgtatct gcgtttcgcg ggccgcgcaa caagcgcttt gcggaatctc
     1201 gatcatctcg agtaagcagg aatgccgcag agtagacttg tggataaagt tccgacgcga
     1261 agcgcgcgcc acgcgctggc gacactcggc gtgcccgtcg cagccttctc ggggtgtcgg
     1321 tacgctgcct ccgctgtgct gcgacccggt gcgatgtgag tgcagaggcc cggcaccgca
     1381 tcgaaaggaa tctccagttc gcgggcgcca ggccgtgctt ttgacggctg cgggggtcga
     1441 ttctacgcgc gttgcggagc cgccgccgtc gcatcgacgg agctgaaagc aacgcgcgta
     1501 gcgtaggatg aagtgcctat ctcacgaaac gcaggttgtc gaacagatag ggagagctgt
     1561 accaaggggc attgacagca atcttgaatc gcagatcggt atagttgccg ccgagagccc
     1621 tcgatacgtt actcggcact gcgaagttga gcgttcggta agtattggct tgcgcgcccg
     1681 cgacactgac ctggcccacg tagaccatga agagccccgc cgacggtgcg tccacgtaaa
     1741 gctgcacatt gccgtacgaa agagaagccg gcgggcgaaa atcgaacgcc aaagttccac
     1801 tcagcggacc aacgttgctg acgggagtag atacgagctc ggtatagtag aagcgggaga
     1861 tcgcgagtgc agaactaccc tgggtggtcg gcgtgctcgc cgtcacggtt ccggacgaaa
     1921 ccgaccattg actcgggttt tcgaatccca gcaccgacgt cgctcccgta cctggagcga
     1981 gttggttgac gatactcacg ttggagacgg cgtgcagatc ggtgagtccg ccgtttgcgc
     2041 cactgaaccc gacatacacg gaaggcggca atgtcaccgt ctggcgaatt acttcggtgc
     2101 cgtccagttc gacgaccagg acactcgggg tggcgccggg aaccgcgttg taaacgcgaa
     2161 cgtgatgggt gaccggcacg tcgccccgca aggccggcac cgcgctggac gtcgcgagcc
     2221 aattgaagtc ttggcttgcg gcgttcaccg ggccgtcggt cagcccgacg aagttggcag
     2281 aaggatcagt accattctgg taggtatcga gtgctaaagc gactcctcgg atgccgagga
     2341 acccgagacc gccgcctcct ccgccgagag ccgttgggcc ctgttgagag gcgtcggcga
     2401 acactagcgc aagcccgtcg gcgccgcttc cgccgcccag gattgcgtcg aagctcaagt
     2461 cgattgccgc cggtgagagc gcggtgctcc agaacgcgct acccgtctga tcaccctcgg
     2521 tggccccggt caagacgaag ctattggcgc tgccgatggc tgaaccgttg aaccgccagc
     2581 ccgaaacgga ccccgaagta gcacctcccg gcacggcggc gccgtgcagc gcgacctcct
     2641 gcaggccatt gtcaccggtg ctgtttagaa cccaaacgtc gtcgtggcta ccctgggccg
     2701 gtggcgtgta cgccacccac tcggtgagcg actcattcac gccgagcacg gtgccttccg
     2761 agagctccgt ggtagccgtg aaggggccca acactggcgg cttcgatttg gtgatggtca
     2821 gcggcactcc gcccgtattg cgaacctcga atttggccca gcgggtcccc gataccgcta
     2881 ctcgtccata gtccacgctg agcggggtga cactcaggtg gcccggcagc gcgcagttcc
     2941 ccgagagggg tacgctggtg tttccggcgg aggtctggat cccgagcgtg ctcgcgtagt
     3001 ttcccaacgc cgtcggggca aactgcagag tcaacgtcaa cgatgcatcg ggcgccagct
     3061 gagtgccggg cgcgggcagt tgggccgccg agaaggggga agttgggctc gtgaacccct
     3121 gcaaggtaag tggcgttgca ccagcgttgc tcaatacgag ttgctgggtc aacgccccgc
     3181 cgacggtggt tccgccgaaa ctcagtgccg gcgccgagag cgcgagttcc gcgctgctgg
     3241 acaagccctt accgctcagc gaagtcgagg cggttcccgc actcgtcgtg aagatcacac
     3301 tcgccccttt gagcccgctc gaagtgggac gaaacgtgat gggcacggtc gtggtagccc
     3361 cggctgccag cgtcggcgac gcgacgggcc ccacgatgaa ctcgggcgaa gagctgagta
     3421 cgttctgaat ggtcaagggc tgcgcggcgg tgagcgtgat attgaccgta gagtcacgac
     3481 cgaccactgt attgccgaat tgagcgggag tggcagtcag gaccggggtg atcggcgaac
     3541 cgaacccgag aacatgaccg tctcgcgtgc cgacgtagac gcggccatcc ccgacgcccg
     3601 gcgggttgaa ctttgcggat tgcccgaccg gtgcctggaa gagcaaatcg aatgctccct
     3661 tggcgttggg aaccgcacga taggcgcgca gctgagaacc ggcaccgctg ccacctggcg
     3721 accagacgat ccagacgatc ccggttccag ccgtaaggcc gtcggaggtc acaatcgggc
     3781 ggctcgaaga gaaaccgaaa gcgtccgtcg tccttcctgc gaagtcgagc gtgggttttc
     3841 cgtctactcc taagccacgc cgataagcgt agaggaagcc ggagctgccg gccgccgcac
     3901 ctccaccgga cgcggtcggg atgtagacgt atccgccgtc acccggccaa actgccggct
     3961 tggaccagac tccgccgttg gcgtcactgc ggaacacgta cgcgtcaccg ccgttgggac
     4021 ccgtgccaat gccgcctagg ctgtcgcggt tcagtaggta cacgtatccc tgcttgccca
     4081 ccgcgacgag caggtgtgga taggccgtgg tcccgaacga atcgggtagt gcgacgggcg
     4141 cgcccgaggc aaagtcggcg tcccagccat ccaactcctg agcatcgtag ggagcgaaga
     4201 agtcacctgc tcgaagtgag ccgttggttt gcacgtcgag ccggacgatg gcctcgccaa
     4261 gtccggacat actgcccgcc tgggagccca agatggggcc cacgggggcc tcgccgttac
     4321 ccgtggagac gaacatgcgg cccgcgccgt cggacatgat gccgcccccg gattgccaaa
     4381 tgcctgcgcc gttgccgctg gtgttcgcga cccaacgggt ttgagacgag ccgtccgtcg
     4441 agactccaac cacccagcct tgccaatcgg gtgcatcaca atgccccccg aacgcggcgt
     4501 aaacctttcc gcccaagagc agcaagcctg ggcgttgcag gtgatgaatc gcgtcgaagg
     4561 ttcttcccgg tgcgttgctg gcggctccct gaatgagcac cgggaagttc ggcttctctg
     4621 cgccgctcgc cacatcgacg gcgtgcgcta accactgggt aacgcccgct tgcgtagtct
     4681 tggcgaagaa ataggccacg ttagtcgcgg agtcgatgac gggggtgccc gtgattccga
     4741 tgttgggcgt caagtcgttg cagcccagcg tggctgggtt gaacggcgta cccacgttgc
     4801 gatgccagcg ctctgcgccg ctctctggat ctagtccata cacgtcgttc gattcggtga
     4861 ctacgaacag ggtgcctttg gatacgagcg gttgcgcgta tacttgcccg ctaacggtcg
     4921 cggagaacag ttgaccgaag gtagatccag acaccaccga gggggaaagt cgcggttgat
     4981 tgccatagtg gccggtgcgc tggtcgtctc cggcctccgt tatggtggtc gtcgagaggg
     5041 cttccgtggt tcgacctagg ccctcgtccg cgttgggagc ggagccttcg tacgaggagc
     5101 agcccactcc ggctgtcagg ttcgtgagcg cagacaatat tagacctgcg gcgccgcgca
     5161 aagcgcggcc gctcgagcag actctcatgg ttctcgatga catactcatc tcggtgccga
     5221 atcattttga ttcggcggtt gaagttggtt tctgcgggga gagcgagacc ggctgacccc
     5281 gtatggacac gtcaattcat gcgtacttcc tgcacgattc gtacgagcgg cacgccactt
     5341 cttctcgccg cgcacgcgcg gcaagcttcg gtctgaattt tcatgtccgg agcgttagag
     5401 ggtgggatgc gccgaaagct tcgcttggct tcaccgatcc gtgcagtcta ccgagttaca
     5461 gacgtagttc cgcttgcgcc agaacatcgg gggtagaact tcacccttag gagtatctcg
     5521 ccaagttaac ggcccgaccg ttgaatcgat aacgacagaa cggagccatc atcccccaaa
     5581 aagacgtgca aacccctttg atccatgccg gattcgtttg cggaccgggg cgtggcaatt
     5641 gggccgtggc cacgagtcaa aatccgataa ggatatatga tgtctaccgc caagcgcaag
     5701 aggcgtccgc tccccgtcgc cgcagcgggt gccttcgttt cgttcgcggg gttgccgctc
     5761 cgacgagcag cgcccgcccg aggcacgcgc ctccaaacat gcccatcgga aacccaaggc
     5821 tcccggatcc gcagcctgat gagtcgaaag acaccacgag caagcctccc acccagaagg
     5881 gtaccagcag cgttccggat gcaccgccga ttgggccgat tcagccggtc ggaaactgcg
     5941 ggcgccgctt cctcgaccca agtcagagcc gccgaaaccc ggactgccca agtcagcgcc
     6001 gcccaagaac ccttctcctg tcggcaattt tcgcccgcca acgacaaaat gaatacagaa
     6061 gtattcgagc tgttcacgct agctggcgca acggagcgac gtcaccgtcg catgcggccg
     6121 aaaatgagca ccttgccttg gggcagcctc gacccgacgc agctcgattc cgattcgcgg
     6181 tggcaacgcg gtttccgtta gatgaaatgg cacacgtcga gatgtgcgcc cggctcgccg
     6241 aagaactcgg cgggggaacc atgtagagtt cgacccacac gccttgattc cgagccccat
     6301 cctagactta ccgacgcttc tgcgagctgc ggattgcgtc gtacgctact tccgcgttgg
     6361 agaagccttg cccattccgt tgctcgcgca ttccgttgct ccacggtacc tatgctttca
     6421 cggtacctat gctttcacgg tacctatgat ttcacgtgcg agcggcgaag caaaagcgag
     6481 agaacccgcg cggagctttt gacttcgcgc gggccctcgc ggtatggcca actgcttgag
     6541 ctctctcagc ggcttactta gagctacgct tagcgcagtg cgcacgctta gcgcagtgcg
     6601 cacgcctagc gcagtgcgca cgcttagcgc agtgcgctca gtgccgctcc gaaattgatg
     6661 tgaaatggtt gttcgtcgag caccagtgct ggcacggact tgacgccgag gcgttcggcc
     6721 tctgcaatgc gagcggcgtc tttcccgaga tggacgacct cgaccgcgta acgatctgga
     6781 tccagggcgc tggcaacctg ctgctcggct tggacgcaca ccgggcatcc cgcatgatag
     6841 aagatggcct tggttttcat gttctattct ccagcagagc agccgcgctg acgtggcggc
     6901 agtagaccgc gcagggttga ttccgtgagc ggcagcgtct gctctgatgc tttcgaacat
     6961 agggaaagcc cattcgccgc ggtagcgggc acttttcggt gcccgtgcca gcctgcgaaa
     7021 tttgggctca aacggcgctc tcgcagcggc tatctttgac ctcacgccat gacgcgccgt
     7081 gcctcgcccc gcatcgattc cgccccctac cgtcaactcg aagacgtact gggatgcaag
     7141 tggtccgtgc tcgtgcttcg agccatcgca agcggagtct gccgtcccgg ggctctggag
     7201 cgacatattg gcggaatatc caccaaggtt ctcggtgaac ggcttcgtaa gctcgaggcc
     7261 tataaactca tcaataaaac tatctacgac accgtgccgc ctcgcacgac ctatgagttg
     7321 acggcaatgg gccaaaaact catcggcatt atcgagcaga ttcgcgcgct ggacgaggaa
     7381 ctccagcacg gcatgccgga caccgaggtc gagtaatccg gttcagcgcg cctcggctcg
     7441 tagcccaatc tcgatccaac gcggcggatt gggccgtgcg ccgaagggac gccgggaaac
     7501 caggaaggtg ctgtcgaaga gattacgcac gttcaagaac atggagaacg gaccccacag
     7561 ctgaacgccg cccgacacgt ccaccaagaa ttgctcgtcg gtaaccagag actgcgagag
     7621 aggcgctcgc cccggcaact cacgcatagc ggagacgtag gtggccgctg ctgagatgaa
     7681 gcccacctcc cgcttttcag cggcgacact ggccttgagc tggtgacgcg gtctatacgg
     7741 catctcgtcg tgcttccgga cctgtccata ggtcggatcc tcagacgtga agtcgttcaa
     7801 gtattctccg cgcgtccacg tgtatgcgac cgtggccggt atctgccaat ctccactcaa
     7861 gcggagttcc tcggtcgcga aggcctcgaa tccgtaggtg cgagctcggc cggcatcggt
     7921 ttggcgatcc agattggcgt cgtcgcagcc gctcgagaat gtgcaaacgc tggtcaggtt
     7981 cgagtagtcg ttgaaatagc caatcaactc aagccgacga gcgccgcgac tgtagcgcgc
     8041 gcccgcctcg taattcacac tgtactcgac tttattctgg ttgatgccgt tcggcggagg
     8101 cgggctgaag ccgcggtaca cgcccccgag caagccgaga tcgtcgttca agccggcgta
     8161 gactccggcg ccgggcacca gcgcccgaac tagccccgtt actcgacctt cgttccgatg
     8221 gtcctcggaa gacatgcgaa tcaactcggt gcgaagcccc ggcgtaaaaa tcagccgacg
     8281 atactggaga gcggccaggg catgcagcgc gacggctgtc gagcgctcgg tgttgacggt
     8341 gttcaccagc gtaggagcgc tgacgggata gagctcgcca ttgcgcatcg catagccgct
     8401 ctcgctgtgg tgtcgatgaa tgcgatcgtc gtgcagccgc actccgagct cgacccgctc
     8461 cgagaagttt ccctgggcga ggtcgacatt caagagcgtc tgcgcgccct gactcacgaa
     8521 cgtgcgttga ttgggtccca catagagtgt ttcggacgcg gccaaactgt cagactcgcc
     8581 gcgtaacacg gcaatcgagt cctggactct gggatcgtca ggcttcaaca gcatatcccg
     8641 aatcaggccg ccctcgaggc ggttcagttt gcgccacgtg cgcgcatact cgtgccgata
     8701 caggctcgtg gtcagcttga ggtgcgccgc tgggtattcg aagcgatgag ttgccagcgc
     8761 gccggtccgg tgattctcca tacggtcgag cgcgctcgcc gggtatcgcc gatagggatc
     8821 ggcgcggaag tcgcggtcgg tcaacccgag ataggtctcg ttggacgctt cttcggcgta
     8881 cgccagcttg atcaagaact cattgcggta gcgatcgctc tcgagcgtat aggaaacttt
     8941 gaccatccag tcatcgcggg tcgagcccgt gtccgccccg ctcggcaggt gtttgaaacc
     9001 ggtgttccag aggcgcacgc cttccaccaa gaagccgagc ttaccgaccg tcgctcccgc
     9061 atgcgcatgc agctgtccaa agccgtattg cccagcagaa atcgtgatcg cgccggcggg
     9121 ttgctcggga atggctcgcg tcaggaaatc gatggcgcca ccgacggttt gggggccgaa
     9181 cgcgacccct gaggggccct tgatcaccct cacccccgcc atgcgggtaa tcagagggaa
     9241 gtagtacgcg gctggcgccg agtagggagc aggtccgaac aggacgccgt cctccatgag
     9301 cacgagcttt ttgctccgat ccgcgtccac cccacgcatc gcgatgttgg ggcgcagccc
     9361 aatcccatcc tcctggcgca cgtaaacgcc cggcgcttgc tgaagcacct gctgaggatc
     9421 ggtatagcga aatcgctcga gctggcgacg gttgatcaga tgggccgagc ccgcggtttg
     9481 cgcggtgcgc gtgcccacga tcgtgacgtc ttggctaggc cgggcgagtg tctctcgtgg
     9541 aggtgcggcg cttgcctcgg ctggcgagtc gatgactcct gatggctgga cgcctgccga
     9601 tgactcgacg gaggctgaag gatcgattgc ggactccgcg ttcggcgcgc tcgcctctgc
     9661 gcttttggtt tcgggctctg tcgtggggga ttccaccttg tcacgatcaa cggcattggc
     9721 gggtggcttc gtttgctccg aagcgtcgcc gcccggagcg gagttcggtg gagtagattc
     9781 cggcggcgat tcagcgggcg gtgtgttgga ctgggcccgt gcgagttggg tgaacagcag
     9841 acatgagagc atggctgcag gagcaagtgg cttcatgcgg tcagaacaca gggcgagtgt
     9901 gagggggggc accgttgggt gccggcaaga cgtactgcgc gggaagaaga ccgtcctcgt
     9961 agctcgtgag atcgaccttc ggatcaatga agggtacacc ccgccgacca ttgagcgcga
    10021 cccgcgactc ggctctgacc tcgaccggac ccaggccgcg tcgctcgaag tcctgcttga
    10081 tatggtgagc gagctgcaaa atgagatcgg gttgacccga catttcgctt tcctgcagcc
    10141 ccgtcagata gtcccgcggg cttacgtaga acgtgcgtcc gttagccttg ttttgcacca
    10201 ggaacgtggt gtggccacct tttgctcgca gcatcacgcg ccaggcgaaa cgcatgccct
    10261 gctcgtgcca aagcacgttc ccgccatagg ccaagaaccg cagtggcaag agcagctgaa
    10321 cgaacgcata gccgcccgcc aagctcagcg cgagcaggcg cctgcgctgc tgcgaagggg
    10381 acacgccggc aaggcgagtc gatgcagatg cgtcgtatgg ccgtgcccgt tgagctcgcc
    10441 tcggcgggag ataggtcgcg aggtagcgcg cgaggtggaa cgagagccgg cgcggccaag
    10501 ctggggagaa gaagacgagc gcggagagca ccatgatgat cggaaacatc ccgatcggga
    10561 agagcagccg agtgaggaca tgaaacccga tcaccacggc gtacgcgaac ggccgggtgc
    10621 gcgaccacaa caagaacacg acgacactgc tatcgaacag gaagccgcac caactcatgg
    10681 ccaaaggcac gagcggatgg gtaaagagcg gtccaagcac cggcaaatcg gtgtttgctc
    10741 cgagccagat gcgaaggggt tgggcgtgca ggagccagtc gctttgcgct ttggcgagcc
    10801 cggcgaagct gtaaacgagc gccacttgga agcgaaacaa gtaaagccac agacacggaa
    10861 tccgttggcg cccgagccgg ggacgcaacc aagcgtcgac ggaccaaatt cggtgggcgg
    10921 gcgaggcggt gagcaagccc gcgagcagta gagccaaata atagtggttg aggtaagtgc
    10981 tgacgtcgat gagctgaacg tacgcaagcc cgagtgcgaa caacgccgaa gccatacgga
    11041 aagcggcccc cgacgcgatg gccaatgcga gaccaatcat cgcatagaac agcgcgtgca
    11101 tgagcatagg gggcaggggt tcgacccacc cgaatcccca gtacttgaaa tgaaactgcg
    11161 gactgacgaa gaagcggtcc acgaagccgt aagcgaggaa gcggaccatc gagaccgcca
    11221 tcacgagccc gaacgcaatc cgaaatgcgg caagccaggc gatgtctact ggttcgaaag
    11281 cggctatcgc cagccgtcgc aggcggctct caatcggtgt caccttctac tcctgcgggt
    11341 agcttcaagt tgagcgggct gccgctgccg aacaggtcag tcttcagaat atcggtgagc
    11401 tgcttgatgg ctcgatatag agcttcgatt tgcgcaggct gcgcacgatc gagctcgggg
    11461 gtctggtccg ccgtttggcg tgcggcggcc aaggcctgcg agatgtcgct cgccaactcc
    11521 gcatggcccg ccgccatcaa ccaatcatcg aatccgagcc ccgcaccgtc gtcgccacag
    11581 ccttggaaca agcgtgaaaa gcccgtcaag ttggcgcgga tcacgctcgt tgcgaaacca
    11641 gcaaagggcg tctcgggact cgtcacggga tgttgcagcg tgtagccggc ggggacaccg
    11701 agcttccaat ctttgacttc ccgctcgagg tacacgagcg cccacgcgag caccgtcaga
    11761 gcttcctgct cgctcgggta accggtggct ccgatgaagg cctccggaaa gccgcccgag
    11821 gtggcgccgt aaattgcgcg aagacgctga gtctgagcaa tcaagtcggc cgcgatcgcg
    11881 actgcgtatg ccgattttct acgcggcagg tcgtcgacga ttccagccca ggcttggcct
    11941 gcgctcgaag ccgcggcgca gttcgtatcg tttcccgcgt agtggctcag gtactcgagt
    12001 gcgtacaggc cgcgaccaga gatcggaatg ccatccatcc cgcgcgtggc gaacgtcatc
    12061 gacgcgagct gttcttccac gcggcagcgg gccgtactcg gccatgcgta gatgaattcg
    12121 cggatgcctt gcccctggta cacgtcctta cccgcggttt cggatttgct cgcgagaggc
    12181 ccgaactgca gcaactcgat ctctccccag accagcatcg cgcggcggaa ctccgcgcga
    12241 gtcggctcgg tcatcagcgc cccttcaacc tcgacggctt cccgcagggc atgactacgc
    12301 tgttcgaagc ggcaaaactg ccagttggcg cattgcacgg cggcgcggcg caaaccctcg
    12361 cgggtgaact cgccgacttc gacggggggg gctccacaaa gatcaactgg gctggcggtg
    12421 gttccgccag agccggatgg catgccgcct tcactggtgc cgcccggtcc gacgggtgac
    12481 gagtcatcac ccccctgcgg gagcgcgtct ggctttccac ctgacgtgct cgtacttgcg
    12541 gcattcgtcg aggacgtgcc ggcggtgcct gcgtgccctc ctgtacctcc gctggtgggg
    12601 tgcggggcgc cggcgtcggt cgtgcgaccc gccgaggagc ccggcgcacc cgcgccaccg
    12661 gacggtgtcg atgcgcgccc acccgtagtg gctgcagtgg ttccgaagtg ctcccgaggt
    12721 gtagccgttt cgtgacaggc tccgatcggc aacacgacgc aacccaccgc ggcggcgagt
    12781 ctcagattgt gagcgcgctt gatcatggat tagggaacgt cgactccgag tagcgccaac
    12841 agaacgccat cagggtggtt gttggtcgtc gcggcaatac gcttaccatc cggcgaggcg
    12901 acaactcgat agaagtgcga cgtagccacc tgcgaggtct ccccgagcgg cgggtacacg
    12961 aggcggccgc cgacccccac gctttcgtcc agttgcccgt cgggaagcgt cacgaagatc
    13021 gcggggttcg ggccgaagcg gccgacgtat accagccgat cgtcggccag cgcgattacg
    13081 tctcggccac ggtcctcggt gttaccgagc ccatattctt cgctctgaat ggccaacgtc
    13141 ccttggcgac cgaagctgcg atcgagcccg ctcggcatga agccgaacga caccagatcc
    13201 acggattgag tcgtactcca gccgaagctc gagctcgtgt tgacggccgt cgcgcgaccg
    13261 tagcccgtcg tgacgtagcg gccattgctc tggcgggtca cgttgtagca ttcggcgatt
    13321 cctccgtcgt cgaggaaggg attgcctcgc gccacaccgg gcgcgccgat tccaaaaccg
    13381 aagttcggat cgggagttcc gttggggagc aggcgaatca cgacgaagtg attgccgagc
    13441 ccatcgccgt agttcgtgta gcccgagctt acgatcgcgc catcgggctc tacgatgccg
    13501 cgtcgagcgc cgtcggcgaa ggtgccgccg gtgttcagcg tgaatacgct gccgccgttg
    13561 aaggcaggat cgagctcacc cgtgctcgcc aagacacgag cgatgtagcg atcgttgtcc
    13621 gtgcgttggg ttgcaggctc gcccgtgacg gcgcccttct tggctggtcc ggcgccgaat
    13681 accacgatct tctccacgcc cgagctcgcg tcgcgagcga taccccaaga gtcgtcgctg
    13741 ggaccggtcg tgcccgtcca gttcgccgca tcctccggag tccagccaaa atccaggcgc
    13801 aacaagccat cttgtccaaa gctttctacg cgctgtccgc tgctgtcgag ccgtagcagg
    13861 gctacgtctt gccccttgcc cgaagcgtca cgcaggttcg cctgtacgat caggtctcca
    13921 ttttcgagct ctacgatccc tagcgattgc tcgttcccgt cattggacac gaccgcgggt
    13981 tcgaccgtgg tgtccgtaac ccgcggtacc aggttcaagc tgagcacgcc acccgtaccg
    14041 aagctctcgt ccaagcttcc gtccacggag aagcgtacga ccgcaatttg gcggtccacg
    14101 ttcgcgtctg cgccaatgtg acctgaggcg tagagcttgc cactcttggc aaaggccagc
    14161 ccgcgcaggt cattgacctg agtcacgacg ctgctcttga gctgagcaat catggctcgt
    14221 tctggttcgg gagcaccgcc cgagctgcct ccctcgccat ccccgccata acctccggag
    14281 cccccattga cggctccacc ctcagagctc gtcgcgccgc cttcgctctg cggcggctcc
    14341 gtgcttccgc cgctcccgat cgggtcagca ccaccctcgg gactcgaggt gctcgttcgg
    14401 ccgccgcgtg cgttcgagcc gccagaggcc ttggagcctc ccgtcgatcc gccaccggag
    14461 ccgccccctc ctttggagga cgccccgccg ctggctttgg ttcctcccga agtaccgcct
    14521 tggccgactg gaccgtcatc gtcatctcca cagcccacca ccagagatgc acccatgcag
    14581 aacgtgagcg ccgcactcac caggttgatt cgaagaacgc gcatgctttc cgccaatacc
    14641 tttcgggcca aaaaactcgg caaccgcttc agggctcagt cgccgcttga ctcgaggcaa
    14701 atacccgaat gcaccagaaa ttgaaattca ttttcatttg caggtgcaaa atctggcgga
    14761 cggaacgcac cactgaccgg ccgtgttcac gaaggactat tgctgcgtgg taatgcccgt
    14821 gtagtaacca tcagggcggt cgggacttga gtggcttgga atttcccgca attgtgcggc
    14881 ccagccgatg acacgagccg tcgtctccac cgcgcagcaa gcaagtccgg ccctgcaagc
    14941 ccccgctgcg cgcgagccct cgtgcttcgt aatcgggtcc gctaatgaaa acggttttcg
    15001 ttttcagttg aaggggatga ggcgaatgat gtaagagcga agggggattt cgagccgaac
    15061 gggtgcgcga ttccgtcgct actttggctc ttgtttcggg aggcgttgca gttccgcggc
    15121 cacctgccgg cgcagcgccg tcgttcttcg cgacagttct tcgagctcca atcgaatgcg
    15181 tgcacggtga ctctcgagct cgacgatgcg cttggccaac ggattggtcg agccgtcgct
    15241 cgagccggcg gccttcaaat gctcacggtg cctctcgagg tcttcgtcga gctgactacg
    15301 ctcctggatg agtctctgtt cgcgttcacg ctccgcccgc aaactcttga cgacgaccgc
    15361 cgcggccgtg agcgcggcgc gcgccgacac ttccagcccg atggccttgg tcatgcgagc
    15421 tagtgcttct tcgctcaact cgtcgagcga gaagcgatcc ttcagcgcct cgcgtacccg
    15481 aagctggcgt tcgctacgcg ctccgggtgc gacctcgaag agcgccagcg cagtgccggt
    15541 gtcgccatca tagtcgagcg cgtctgcccc gctaatttct gcgtttctaa ccgtgccgcg
    15601 caggccgtag gcgatggtgc gtcgggcagg gctatggttc tcgaaggaga gcgtcacctg
    15661 acgagtcttc acgtagtgct cgtccaaggc catgtcgtga aaggcgacct gcagcgtctc
    15721 gcgcgtgctc tcgcgagtct gttctcggaa gtccgtatcc aaatccagcc catagttcag
    15781 gaacactcgc ccgccgggac gcaggcgggc cagcaccgtc tcgccggaga agcccgcgct
    15841 ttcgaagaca gcgagtggac cggcgggcaa ggtatttcgg gacgcgttga ccagtcgaat
    15901 cgtggctcgg ccttgcgtct cgccggcctc gaatcgggtg agcctcttgg cgtcgagggc
    15961 ctggtcgaag aagggcagga gagcggaacc gtgggctctg agcgatatgg gatctgcaag
    16021 tcgataggag aacagaaggc tttcctgaac tggtttcgct ttggcgacgc tcgccagatc
    16081 accgatcgat atcgagtcac tcaccaccgc gcgcgctgcc atagggtggg cggtggcgat
    16141 gcctccggcg cctgcgccgt acccgacacc gcccaccgat tcgatgtcgg caagaccagc
    16201 gccggttccg tcatcctctt cctccgcatg gtctccccag atcgcgtccg gtgtcttgag
    16261 tgcgagttgc ggaaccgtag agaggcgctc ctcgggggtc gtcatctgcc tgcgggcgta
    16321 gcgaggcgct gccatcggga acaggaatga atcgggttgg ccatttacca actcgatgtt
    16381 gacccgagcc catgcctcat ccgtatcgtt atggatgagc gcccagcctt ggaccatggc
    16441 cgcgtcacgc tcggcgtcta ggaccaaacg atagctagct cgccaaaccg gggcctccgc
    16501 aacgtacccc aaacgcacgg gagcccctgc cgcggcgagc acgcgcaact gacgcgccag
    16561 ctgtgccgcc cttccactga gcgcgtcggt agccgttcgg agccgtgaag aaaagcctga
    16621 atccaacgga aggatctcgg tggtgtcgga ggcg
//
LOCUS       DASTKU010000154         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_29529,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000154 DASTKU010000000
VERSION     DASTKU010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29529"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="VFQ61_05545"
     CDS             <1..1178
                     /locus_tag="VFQ61_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018555222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HET9953942.1"
                     /translation="EEFCALAGHEHVHKGMTSRDLTENVEQMQVRDGLKLIRERMVSA
                     LVRLASRAAQYSELVMAGRSHNVPAQATTLGKRFANAGEELLQAFVRLESLIDNYALR
                     GIKGPVGTQQDQLDLMGEDAQKVSDLEARIARHLGFSHSLTNVGQVYPRSLDFDAVSA
                     LFQCVAGPSSLATTIRLMAGQDLVTEGFKAGQVGSSAMPHKMNTRSCERINGFRHILA
                     GYVTMLTGLAGDQWNEGDVSCSVVRRVALPDAFFAADGTFQTFLNVLDDFGAFPAVIE
                     RELARYLPFLATTKILMASVKAGVGRELAHEVIKEHAVASVLAMRDRGAEQNQLVARL
                     AADARLRLSHAELQKALAQPLEFVGAAPAQARAFVTQVEALSRRFPEAASYSPEPLL"
     gene            complement(1570..1950)
                     /locus_tag="VFQ61_05550"
     CDS             complement(1570..1950)
                     /locus_tag="VFQ61_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953943.1"
                     /translation="MHRDLPRAVAHALPLTFVTLLVVPSYEGGSWGRLSDPACQLLVR
                     LAGFVDPRSSALSPLDRWSVASLRGGVESIPDFSELLHSEEFDAACAARTRQAGADMS
                     SFTRPNEEPPKRDVLTQEMAVTAW"
BASE COUNT          562 a          787 c          840 g          459 t
ORIGIN      
        1 tcgaggagtt ctgcgcgctc gccggacacg aacacgttca caaagggatg acctcgcggg
       61 acctaacgga gaacgtcgag caaatgcaag ttcgggacgg gctcaagttg atccgtgagc
      121 gtatggtctc ggctctggtt cgcttggctt ctcgcgccgc ccagtactcc gaactcgtca
      181 tggcagggcg gagtcataac gtgcccgcgc aggccaccac gttgggtaag cgcttcgcca
      241 atgccggcga agagcttctc caggccttcg ttcggctcga gtcgttgatc gacaattacg
      301 cgctccgcgg gatcaaggga cccgtaggta cccaacaaga ccagttggat ttgatggggg
      361 aggacgcgca gaaggtcagc gacctcgaag cgcggattgc ccgccacttg ggcttctccc
      421 atagcctcac caacgtcggg caagtctatc ctcgctccct ggattttgac gcggtgagcg
      481 cgctgttcca gtgcgtggcg gggccatcta gtctggctac gacgatccgt ttgatggctg
      541 gtcaggatct cgttacggag ggcttcaagg ccggccaggt cggatcctcc gccatgccgc
      601 acaagatgaa cacccggtcc tgcgagcgga tcaacgggtt ccggcatatt ctggccggct
      661 acgtcactat gctcaccggg ctcgccggtg accaatggaa cgaaggagac gtgagctgtt
      721 cggtggtgcg gcgcgtggcg ctaccggacg cattcttcgc ggcggatggc acgttccaaa
      781 ctttcctgaa cgtactcgat gattttggcg ctttcccggc ggtgatcgag cgcgaactag
      841 cgcggtatct gcctttcctc gccacgacca agatcctcat ggccagcgtc aaggcgggag
      901 taggtcgaga gcttgcgcac gaagtgatca aggaacatgc ggtcgcttcc gtactggcga
      961 tgcgggaccg aggcgcggag caaaatcaac tcgtggcccg cttggcggcc gatgcccgcc
     1021 tgaggctcag ccacgcagaa ctgcagaagg ctctcgccca accgctcgag ttcgtgggcg
     1081 cagcaccggc tcaagcgcgc gcgttcgtca ctcaggtgga ggccctcagt cgtcggtttc
     1141 cagaggccgc ctcgtactca cctgagccgc ttctctgagg cgtgggggag gcgagcgtgc
     1201 tccgacgatg accttgcgtg cacacgaggt catcgtcttg ctggcgcaaa cccgaggtgg
     1261 ggcgagctag ctcgagctcg gtgcgggatt tggcacacgc cccggcctcg gggcgagtga
     1321 agctcgggag gttgagtccc ttcacgcggt gaagcgtcga ctaggacttc actgttggca
     1381 ttccacgctc agagcacccg ctgcgtgagt cgatcgcgtc cggtcgcgcc gagaagtgtc
     1441 cgagcccgtc tgttttcgaa ccctctgccg gttcgagaac cccgctacag ggcgctttcc
     1501 cgctcctcca cctcagcgtg gaaatgatgt cgggcgggac atgcatcgcg ctgcgcagat
     1561 gccgcgccgt taccacgcag ttaccgccat ctcctgggtg agtacgtcgc gcttgggcgg
     1621 ctcttcatta ggtcgtgtaa aactactcat gtccgcaccg gcctggcggg ttcgggcagc
     1681 gcaagcggca tcgaactcct cagagtgtaa caattcagaa aagtccggga tggattcgac
     1741 gccgccacgc agcgatgcga cactccatcg gtccagcgga ctcaacgcac ttgagcgcgg
     1801 atcgacgaag ccggccaaac gcacgagtaa ctggcacgcg ggatcggaca agcgtcccca
     1861 cgagcccccc tcataagaag gcaccacaag cagggtgaca aaggtgagcg gcagcgcgtg
     1921 cgcaaccgcg cgcggcaaat cgcggtgcat cgcggtgcat acagaggagc agtagtcaga
     1981 gagtgctggg actctcttgc gagacacatt cgacaaaatt gtgatcgagc gaagctcgat
     2041 atgccctgca ggctagtatg cgattcgcct gttcggaggc aggcgccagc agtgcatgcc
     2101 ccattcgtga atcagcgctg caggatggtg gctgtcgcaa cgagagcctg agctccaaca
     2161 ctaaaccttt gcccaagttc ggctgaagct agcggccgaa actcggagta gccggatgta
     2221 gcttcttagc cacctgctac gccggacgac ctacgacatg caggaaaaga cagccacaaa
     2281 cgatagattc gaaggagaca ctcaaatcga gcgtggcaaa ggacaagccc cgaagcggcg
     2341 actggcgcga tgaatgcagg ctgccgacaa gaggcctcat gcgggcggcc gaatgatgga
     2401 cgtgaatggc aaaagaaact gagccactcg aaaagagcct cactcgaaag cgagtcgaac
     2461 ctacgaatgc ctcgggaaaa tcagcagaac atcgaaaaaa acagcagaac tccagatgtc
     2521 agcagcgcct cacgccaggg gaaaggcgcc caggcaatgg gcaggaggcc caagcgattg
     2581 aggcaggagg cccaagcgat tgaggcagga ggcccaagcg attgaggcag gaggcccaag
     2641 cgatgcgg
//
LOCUS       DASTKU010000155         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1813591, whole genome shotgun sequence.
ACCESSION   DASTKU010000155 DASTKU010000000
VERSION     DASTKU010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813591"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VFQ61_05555"
     CDS             complement(<1..1065)
                     /locus_tag="VFQ61_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9953944.1"
                     /translation="MSFATAQPKPETEVSEGSTFAAPASSPSPSPSPSSPPSSATSSM
                     LGPSGDDELQARGLKSVLVVDDDREMCELAEATLGRRGYSVTWRVSPEVALALLDEQD
                     FGVLLVDIHMEGMNGLDLCRAALAKRPDLVVIVMTGFGSIDHAVGAMRAGAYDFITKP
                     VAMDALAITVERAARHRAMSDELRRLRGLVNDRELPNMLGKSPAMQRLGDLVARVADT
                     STSVLITGESGTGKELVARAIHERSGRTGPFLAINCAAVPENLLESELFGHVRGAFTD
                     ARSARSGLFVEASQGTLFLDEIGELPLGMQAKLLRAIQERKVRPLGAAQELAFDARII
                     TATNRNLEHEVEEKRFREDLF"
     gene            1368..2120
                     /locus_tag="VFQ61_05560"
     CDS             1368..2120
                     /locus_tag="VFQ61_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953945.1"
                     /translation="MTSSRPDPGSPEARLALLDELRNAERRAAISRVASIIGHLIGTP
                     LQVISGRTWLIRSNLTPEATLENARRIEEQVERLAQRVQQLIQYLTTPEADQDEPVTV
                     QELLSQALALYSPIAASRGLHIVLHSSLPAPGSAEVGHRLPGTSTLILLTSLLSLAIR
                     LAPAGVCIELEASASSENHVILALRIPGMAVPKTRIDRLEPPEQQESLTADDLQVLSV
                     CHAIARRIGGKFEVVPVDASRATIQFECAAIP"
     gene            complement(2138..2707)
                     /locus_tag="VFQ61_05565"
     CDS             complement(2138..2707)
                     /locus_tag="VFQ61_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET9953946.1"
                     /translation="MRIPKELLDLDLYALFDLDSSATPAEVRRAYRYKARQSHPDLNP
                     EDESAEQRLALLNRAAHILLDPGLNRSYLRTRARTIHREPDWFERCNPAHEPGEDLDW
                     GSPASRAPAHRKRPSAAGRAGFSRRVRDWNARCAALVGGWVPFENPSHALVLTALCLV
                     LGSGLIACARPSNAFWSASFWSAHELHLP"
BASE COUNT          525 a          867 c          884 g          518 t
ORIGIN      
        1 gaacaagtcc tcgcggaatc gcttctcctc gacctcgtgc tcgaggtttc ggttcgtggc
       61 agtgatgatt cgggcatcga aagccagttc ttgggccgca cccagcggac gaaccttgcg
      121 ttcttgaatg gctcgcagga gcttggcttg catgccgagc gggagctcgc cgatttcgtc
      181 caggaacagc gttccctgac tggcttcgac gaacaggccg gagcgcgccg atcgagcgtc
      241 cgtaaaggcg ccccgaacgt gcccgaacag ctcactttcc aacagattct ctggaaccgc
      301 cgcacagttg atcgccaaga acggccccgt gcgaccgctg cgctcgtgaa tggcgcgggc
      361 tacgagctct ttcccggtgc cgctctcgcc agtaatcagc acactagtgg acgtgtcggc
      421 gacgcgcgcc accaagtcac ccaagcgctg cattgccggg ctctttccga gcatgttcgg
      481 gagctcacga tcgttgacca aaccgcgtag gcgccggagc tcatcactca tggcacgatg
      541 gcgggcagct cgctccacgg tgatcgccag cgcgtccatg gcgacgggct tggtgatgaa
      601 gtcgtaggcg cccgcgcgca ttgcacccac cgcatggtcg atgcttccga acccggtcat
      661 cacgatcacg acgaggtccg gccgtttggc aagcgcggca cggcagagat ccagcccgtt
      721 catgccttcc atgtgaatgt cgaccaggag aacaccgaag tcctgctcat cgagcagcgc
      781 gagagcgact tcgggagaaa cccgccacgt gacggaatag ccgcgccggc cgagcgtggc
      841 ttccgctaac tcacacatct cgcggtcatc gtcgaccacg agcacggact tcagtccgcg
      901 tgcctgcagc tcatcatcgc cgctcgggcc gagcatggag gaggtggcgg atgaaggcgg
      961 agaggagggt gacggtgaag gagaaggtga cgaggcaggt gcggcaaagg tggacccctc
     1021 agagacctcc gtttcgggct taggctgagc cgtggcgaat gacatggtgg cgtccctggt
     1081 gcatgctgcg tgccgccccg tgggaaaaag aaaagcgtga aagaacggga ccttatcaac
     1141 aggggtaccg ttgcattttg tcacggtggg gcaaaagtgt gtgacctcaa tgctgagtga
     1201 ggcgacgctg ggcgccacgc tgccttgagt gcgcaaatgt aggcaatcac agtggaagca
     1261 gacggtcgga gcaccggcgc tgagcgctgc acagcttcgg gggccgcgca tccaggagga
     1321 cgacgcgatt gtaacgaata cgtgtgtgat agggtggccc gagggtaatg acgagctcac
     1381 gtcccgatcc cggctcgccc gaagcgcgct tggctctctt ggatgagttg cgcaacgcag
     1441 agcgtcgggc tgcgatctcc agagtcgcct caatcattgg gcatctcatc ggtacaccgc
     1501 ttcaagtcat ctccggcagg acctggttaa tccgctcgaa ccttacgccg gaggccacgc
     1561 tggagaatgc tcgacgcatc gaagagcagg tcgaacgttt ggcgcagcgg gttcagcagc
     1621 tcattcaata tctgaccact cccgaggcgg atcaggatga gccggtaacc gtacaagaac
     1681 tgctctcgca ggcgctcgcg ctgtactcgc cgatcgcggc cagccgcggg ttacacatcg
     1741 tgttgcattc gtccctgccg gcgccgggta gcgcagaagt cgggcacagg ttgccgggga
     1801 cttcgactct gatcttattg acgagcctgc tttcgcttgc cattcgtttg gccccggcgg
     1861 gcgtttgcat cgagctggaa gcctcggcgt cgtccgaaaa ccacgtgatt ttggcactgc
     1921 gaatcccggg catggccgtt ccaaagacca gaatcgatcg cttggaaccg cccgagcagc
     1981 aggaatcact cacggccgac gacctccagg tactctccgt atgccacgcg attgcccgcc
     2041 ggattggcgg gaaattcgag gtcgtccccg tcgatgcctc acgcgcgacg attcagttcg
     2101 agtgcgccgc catcccgtga acctggagcc aagcgcgtta cggcagatgc agctcgtgcg
     2161 cgctccagaa gctcgcgctc cagaaggcat tgctcggccg tgcgcacgcg atgaggccag
     2221 agccgagtac cagacaaagc gcagtcagga ctagcgcgtg cgacgggttc tcgaacggga
     2281 cccaccctcc caccaaagct gcgcatcgcg cgttccagtc gcgaactcgc cgtgaaaagc
     2341 ctgctctccc ggcagcgctc gggcgcttcc gatgcgcggg tgcgcgcgat gctgggcttc
     2401 cccagtcgag atcttctcct ggctcgtgcg cggggttgca gcgttcgaac cagtcgggct
     2461 ctcggtgtat cgtccgagcg cgggttcgga gatagcttcg attcagcccc ggatcgagca
     2521 ggatgtgtgc agcccgattc aggagtgcca agcgctgctc cgccgactcg tcctcagggt
     2581 tgaggtcggg atggctttgt cgagccttat agcggtacgc ccgccgcact tctgccggcg
     2641 tcgcgctcga atccaaatcg aatagcgcat acagatctag atccagcagc tccttcggaa
     2701 tccgcatggg aagcgtcgct cctgtttcaa actttgcgta gctcgcgagc gctcttctcg
     2761 cgttcgtcga ggcgtggtga gccgacagcc tccg
//
LOCUS       DASTKU010000156         9643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_29864,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000156 DASTKU010000000
VERSION     DASTKU010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9643
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29864"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VFQ61_05570"
     CDS             <1..864
                     /locus_tag="VFQ61_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HET9953947.1"
                     /translation="ELNDDIRKDGLKLPGGVFPEHFGLPVGGVFEAYLPHIPSQVARC
                     NCGSGAHGSPAPWDIENAPGVPQVGPVESQAIRRQVAAELAARQRARGDVPAGWQRWA
                     KQVLEPTIDWRQHLAGAVREAVAWASGAVDYTYSRPSRRQRAIPGIVLPSLRRPLPRV
                     AIVIDTSGSMSDVALGAALSEVTGVLRAVGVGTNELEVWACDAAVQSSQRVRQISEIT
                     LKGGGGTDMTVGIQRALAVPRPPHVVIVLTDGLTPWPEVPSRTRIIAGLIGKDPPQPP
                     GHIEAVRIPRE"
     gene            complement(938..2320)
                     /locus_tag="VFQ61_05575"
     CDS             complement(938..2320)
                     /locus_tag="VFQ61_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9953948.1"
                     /translation="MVDRRDLETCLRVLAELRAAGDKHPDHAVIEREVAAFYKGGKKR
                     RRVAREQARSARDRARLEATARVMQTLSPEAASEPRSTRDPESNEEYERARSCYVCKQ
                     PYRRVDTHYHMLCPVCANRHKTERARRADLTGRRALVTGGRIKIGFELTRMLLRDGAA
                     VTLITRFPQDAVRRFQALPEAATFGERLAIVGLDLRYLPAVVEFCSAWLARGEALDIL
                     VNNAAQTVRRPPAYYRELAAGERALISGRETASPSADSRWLEAHNSGRGLLDHFHEQC
                     LQDRDCDAFPIGLRTEEGLPLDLRERNSWRLALADVDTREMVEAQLVNAMAPFVLMGK
                     LRPALERSAFSPRFIVNVSAMEGKFDYRAKTSRHPHTNMAKAALNMLTRTSAADYAES
                     EIYVNSVDTGWITDENPEPLKLRLRENGFHPPLDVVDGAARIYAPILDGLAGKPYFGQ
                     FLKDFSPTTW"
     gene            complement(2427..3347)
                     /locus_tag="VFQ61_05580"
     CDS             complement(2427..3347)
                     /locus_tag="VFQ61_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953949.1"
                     /translation="MGTRHAIVVRPEPEQDYVVYPGSAGLAWTQDHLTLAFVYWQDHA
                     SAVSLDLEARQLAATSLGLAGYMFLDPFTVIAYDNWFAAAWVRWHIGDDKSLSISTSG
                     SNVPDAGFAIYSRGFGAPTDYGASLTQLGESLVASATTQRSSSDIGQEEGPRCRYVVY
                     GAAGELGSSSVLGACKGTHRLLGRSGHAVLVQAQSDGLKLARINEQGQLTTPLLKIAP
                     PSAESASILDAGPGLAGVLWSDGDAGHFVLIDDTTLQALSNVVTFYASAGSRVANPLG
                     VYSAGDLLAVFDRDGGADAIAAHCMASLPN"
     gene            complement(4091..4459)
                     /locus_tag="VFQ61_05585"
     CDS             complement(4091..4459)
                     /locus_tag="VFQ61_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953950.1"
                     /translation="MPAVQVLAALDPAARVEERRLTLQEALGMAKAVEAQERARAMAE
                     PPRWAERTPSSLRGATAEATFKQVLERAGPGLLELGWLKVGRRLRAQLEPLPEPRRAE
                     AEERAPEDTPAARATAAARI"
     gene            complement(4661..6847)
                     /locus_tag="VFQ61_05590"
     CDS             complement(4661..6847)
                     /locus_tag="VFQ61_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953951.1"
                     /translation="MGKSCMRISGRLPIGHGFLERTPLWSAIAAVGLAVACSTSSPES
                     PGSGGHTSSGGSGGRISGGGGTKNGGGQSSTGGMQTSSAGGDDSAGGGAAGETSVGGT
                     GAGTTEGGSSNSSSQGGSFTQAGDTGLGGTIESGGTASGGTSAAGATAGGMTAGGMTA
                     GGMTAGGGAPLGGSGGTVIGGESGAGGQGGSTSEPPATEPCTGAVRIRVPPTTGMSLT
                     VTREALRVTSSTQEVVPGPILVQANQGSATGNTEWCASGESSCNGFCNMATCPCIHCA
                     PTEDYTTNPVRAADLMAGVDALIPQNANNIQASCIGENCSVLEHLEPSDPYSALLNVG
                     ASQAPTDFAIEFEYETMLGSCQWAQPATRAFNCAAGKTLSVSDKPWLQKSLVMTPEGP
                     VVAYSEATVRPPPPVDPLPPFDSSLAKISFEAEMGTRHPIFITLERDVVAYPSSAGLI
                     WTGDHLTLAFNYYWATASAVSLDLQATQLGDVVTIGQANDIFREPFTAIPFGTSFATA
                     WVWRRTFGEEWLQVFTHEPGFPQPLIPMYTSSSGTKDYGASLARAGQSLVISTTTQRS
                     AMDIGQDEGPRCQYIVYDASGELGSSSVLGACMGTHRLLGRSQHAVLVQTQSDGLKLA
                     RLDDRGQLTTPLMKVASVFTASPSLLDAGPGLAGVLWSDGEAGHFALIDDTTLEVRSN
                     VVTFYASAGGKVEAPLGLYSAGSLLVVFDRDGGADALPVRCAEPLN"
     gene            complement(7204..8208)
                     /locus_tag="VFQ61_05595"
     CDS             complement(7204..8208)
                     /locus_tag="VFQ61_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953952.1"
                     /translation="MKRDASPGGGKSQGTPAEPEAREARELREAELRRILIRSATLDR
                     PSAASRRQALDATLRRLETSHPGSSHRSPWLVLLAAAVVGIVLLSVRFSGPWFSDGAE
                     RGPGLIRKEAAFVPAPRDDNAAKPGHTVGLIACPARSSPRGVGLSIDSFEDGDAQLEL
                     LDGRSGTWIFADDGTGRRTRTEPLSSSRVMHPTRMLARAASQYALHFSGPRLTLWGAS
                     VSSLLAPEKCYDVSAYGGVGFWTRGRGRIYLGMPILDVVPRSLGGTCERGCNNVHRVA
                     VDLSPDWTYHVARWPDLLQMERGLPLEFDPHRVFSVEFTVFAQDTPFDAWIDDVSFVD
                     "
     gene            complement(8205..8813)
                     /locus_tag="VFQ61_05600"
     CDS             complement(8205..8813)
                     /locus_tag="VFQ61_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9953953.1"
                     /translation="MAEYVVEGPLHTCSVQTFEGSDAVADGGSSCPLRAFVEEYFDFA
                     WRTLRRFGIPEPDLEDAVQEVWIIVARKRASILPGRERAFVFGTAGRVASNYRRRCRR
                     RPQELGGTLEHAPCIAQDPEEIAQLQHARPILQGILDRMTDTQRAVFVLCDLEDQAPG
                     AVATMLGIPVGTVNSRLHRAREIFNSAVKRLVERENLGHARP"
     gene            complement(8912..>9643)
                     /locus_tag="VFQ61_05605"
     CDS             complement(8912..>9643)
                     /locus_tag="VFQ61_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953954.1"
                     /translation="DEHPAVALGSAVASATAAGPVSTATMSNREPGTQDAPSVPSLPA
                     CRKVVVAQGDAPLIDDLEDGNSRLQLADGRSGFWFVHNDGSGTQTPSADRVLFPSLIS
                     PPRGRSQWALHTSGSGFKKWGALFGNFLAERACYDSSAYDGLEFWAKGFGTVHLVVWT
                     VDIVEPYEGGFCQKDCYNGYKKRIELGPRWSSYSVPWGDLAQLRAGPKLEFDPHRVVF
                     LGFVVGPDVPRFDVWIDDIRYVKRK"
BASE COUNT         1852 a         3123 c         2904 g         1764 t
ORIGIN      
        1 gagctgaatg acgatatccg aaaggacggt ctgaagttac cgggtggggt gttccccgag
       61 cacttcggtt tgcccgtggg gggcgtgttc gaggcgtact tgccgcacat cccgagtcag
      121 gtagcccgct gcaactgcgg ctcaggagcg cacggctcgc ccgcgccttg ggatatagag
      181 aacgcgcctg gtgtcccgca ggtggggcca gtcgagagtc aggccatacg acgccaagtc
      241 gcagcggagc tcgccgcacg gcagcgcgct cgcggtgatg tcccggcggg ctggcagcgg
      301 tgggccaaac aggttctcga acccaccatc gattggcgac agcacctggc cggagcggtg
      361 cgtgaagccg tggcgtgggc gagcggcgcg gtcgattaca cgtattctcg tccgtcgcgg
      421 cgccaacgcg caatccccgg catcgtattg ccgagcttgc gtcgtccgtt gccgcgggtg
      481 gcgatcgtca tcgacacgtc cggatcgatg agtgacgtcg cgctcggggc ggcgctcagc
      541 gaggtaacgg gtgtcttgcg tgcggtcggc gtcggcacca atgagctgga ggtttgggcc
      601 tgcgacgcgg ccgtccagtc gagccagcgc gtgcgccaga tttcagagat caccttgaaa
      661 ggcggaggcg gaaccgacat gacagtgggg attcagcgcg ccctcgcggt gccgcgcccg
      721 ccacacgtgg tgattgttct cacggacgga ctaacaccct ggcccgaagt gccttcgcgc
      781 acccggatca tcgccggtct gatcggcaaa gatccgccgc agccgcccgg gcacatcgag
      841 gcagtacgta ttccgcgaga ataggccgct agagtcgcac gctactccag cgttgccatg
      901 atcgggagcg cgccccgcga cgccacctca cctcacctca ccaagtggtg ggggagaagt
      961 ccttcaagaa ctgcccgaaa tagggcttcc ccgctaggcc atcgagaatg ggcgcgtaga
     1021 tgcgcgcggc tccgtccacc acgtcgaggg gagggtgaaa gccgttttct cgcagccgga
     1081 gcttgagagg ctcggggttc tcgtcggtga tccagcccgt atccacgctg ttgacgtata
     1141 tctcggattc ggcgtaatcg gccgcgctcg tgcgcgtcag catatttagc gcggctttgg
     1201 ccatattcgt gtgcggatgc cgcgaggttt tggcgcgata gtcgaacttc ccctccatcg
     1261 cggaaacatt cacgatgaag cggggtgaga acgcggagcg ctcgagagca ggccgcagct
     1321 tgcccatcaa cacgaacggc gccatcgcgt tgacgagctg cgcttccacc atctcgcgcg
     1381 tatcgacgtc tgcgagcgcc agtctccagc tgtttcgctc tcgcaagtct agcggcaacc
     1441 cttcctcggt tcggagccca atcgggaaag catcacaatc gcggtcttgc agacactgct
     1501 catgaaagtg atcgagcaag cctcgccccg agttgtgggc ctcgagccag cgcgagtctg
     1561 ccgagggcga cgctgtttct cggccactga tcagcgctcg ttcccccgcc gccagctcgc
     1621 ggtaataggc aggcgggcgg cgcaccgtct gagccgcatt gttgaccaga atatcgagtg
     1681 cctcgcctcg cgcgagccac gcagaacaga actccacgac ggcgggcaag tagcgtagat
     1741 cgagccccac gatggccaag cgttccccaa aagtggcagc ctccggcaac gcctgaaagc
     1801 gacgtaccgc atcttgggga aagcgcgtga tcagcgtgac ggcggcgcca tcgcgcagca
     1861 gcattcgagt cagctcgaag ccaatcttga tgcgcccacc cgtgaccagc gcgcgccgcc
     1921 ccgtcagatc ggcccgacgt gctcgctccg tcttgtgtcg attggcgcag accgggcaaa
     1981 gcatatgata gtgagtatcc accctacgat aaggctgctt gcaaacgtaa caacttcgcg
     2041 ctctctcata ctcctcgtta gactcggggt cccgggttga gcgaggctcc gatgctgctt
     2101 ccggtgagag cgtttgcatc actcgggctg tcgcctccag acgcgctcga tcgcgcgccg
     2161 agcgagcttg ctcgcgagcg acccggcgtc gtttcttgcc gcccttgtaa aatgccgcga
     2221 cctctcgctc gatcacggca tgatccgggt gcttgtcgcc cgcggccctg agctccgcga
     2281 gcacacgcaa gcaagtctct agatcccgtc tgtccaccat gagggagcta cctcgtaccg
     2341 cggtcggaat caacgatccc ggcgcggggc gccggatggc tcgcggcgcg ccgtagcggc
     2401 tccgagtgcg ttctttggcg cccgcctcaa ttgggcagcg aagccataca atgagcggct
     2461 atcgcatcag cgcctccgtc gcgatcgaac acggcaagta gatcccctgc cgaatacacc
     2521 cctaaaggat tagcgaccct actgccagcc gaggcgtaga aggtcacgac attgctcagc
     2581 gcctggaggg tggtgtcgtc gattaacacg aagtggccgg cgtcaccatc ggaccacagc
     2641 acgcccgcga gaccgggacc cgcgtccagg atgctcgccg actcggcaga gggcggggcg
     2701 atcttcagga gcggggtggt gagctgccct tgctcgttga ttcgagccag cttcagcccg
     2761 tcagattgag cttgcacgag caccgcgtgt ccgcttcttc cgagcaagcg gtgtgttccc
     2821 ttgcaggccc ccaaaaccga agacgagccg agttctcccg cggcgccgta cacgacgtag
     2881 cggcagcgcg gtccttcttc ttgaccaatg tccgaggagc tgcgctgcgt ggttgcggat
     2941 gccacgaggg attctcctaa ctgggtcaag gaagccccat agtccgttgg tgcaccgaac
     3001 cccctgctgt aaatagcgaa gcctgcgtcc ggaacattgg agccggacgt ggaaatactg
     3061 agcgatttgt cgtctccaat gtgccaccgc acccaggctg cagcgaacca gttatcgtac
     3121 gcgatcaccg tgaacgggtc gaggaacatg tacccggcca acccgagact tgtcgccgca
     3181 agttgccgag cttccaaatc taggctcacg gccgacgcgt gatcttgcca gtacacgaac
     3241 gccaaggtca ggtgatcctg cgtccaagct aggcctgctg aaccagggta aacgacatag
     3301 tcttgctccg gctcggggcg cacgacgatt gcatgtcggg ttcccacaga gccgtcaaaa
     3361 gagatctttg ctagactaga gtcgagtgga gagggtggtg gtccgacggg ttgcgggcgt
     3421 accgtcgctt cactgtacgc gacaacgggc ccttccggcg tgagcactag cgatttctga
     3481 agccagggct tatccgaaat cgccagtgct gtcttcgtga gttcgcaact aaaaggtcga
     3541 acgcgtgggc ctagcccagg gcacgatccc agtctcgtct ggtactcgaa ttcgaacgca
     3601 aactcggttg cctcgatgga agcatctgcg gtcattactg cagagtattg gccagccgcc
     3661 tccacggtct ccagtaccga gcaactctcg cccgcacagg aggcgtgcac ggcagtggcg
     3721 ctctcgggaa tcagactttg gatacttggc atcaaatcgc cgacaagtac cggattgata
     3781 gcgaaatcct ctggtgctgg gcatcccgga gggcagggac acctagcttc attgcaccag
     3841 cccatacagc tcggttcacc gctggcgcac cactcggtgc taccggtcgc cgcaccttgc
     3901 tgcgctacca ccagtatggg tccaggcacc actggttgcg cgctcgaggt gacccgaatc
     3961 gcgtctcgcg tcagtgttag agtcatgccg gtggatgcgg gcactcgaac gcggacggct
     4021 cccgtgcaag tctccgtttg aatcggttcg gatgaacttc ctccttgatt gagtgcgccg
     4081 gcctcgccgc ctaaatccgt gccgccgccg tcgcccgcgc tgccggcgtg tcctccggcg
     4141 cccgctcctc cgcttccgct cgccgcggct ccggtagcgg ttccagctga gcccgtagtc
     4201 gacgccccac cttcagccat cccagctcca gcaagcccgg ccccgctctc tccagcacct
     4261 gcttgaacgt tgcttccgcc gtcgcccccc gtaatgacga gggtgttcgc tccgcccatc
     4321 gaggaggctc cgccatggct cgcgcccgct cttgagcttc caccgccttg gccattccca
     4381 gcgcctcctg cagtgttagt cgccgctctt ccacccgagc cgccggatcc agggccgcca
     4441 gcacctgaac cgctggcacc aggagtgcta tccgcacaag aagccagcac ggctagagct
     4501 gtcagccacg agcacaacgg cgccctactc agcggacctc gggacttgga agggagtggg
     4561 atgtactcca tcgaaagctg agccatgact ctccggaaat atgaatgaag gaatgcctcg
     4621 aactggctac gaatcgtgtt tgccggacct cacaccaaga tcaattcaga ggttctgcgc
     4681 aacggaccgg taaggcatct gcgccgccgt ctcgatcgaa caccacgagt agagacccag
     4741 cggagtatag tcccaaggga gcctccacct ttccaccagc ggaagcgtag aaggtcacga
     4801 cattgcttcg cacttcgaga gtagtgtcat cgattagcgc aaaatggcca gcttcaccat
     4861 cggaccacaa caccccagcc aaacctggac ccgcatcgag caaactggga gacgccgtga
     4921 acaccgaagc aaccttcatc agaggcgtcg tgagctgccc acgatcgtcg agtcgcgcga
     4981 gcttcagtcc gtcactctgg gtttggacga gcaccgcgtg ctggcttcgt ccgaggagtc
     5041 ggtgcgtgcc catacaggcg cccaacacgg aagaggagcc gagctcgccc gaagcatcgt
     5101 acacgatgta ctggcagcgc ggcccttcgt cttgtccgat gtccatggcg ctccgttgag
     5161 tcgttgtgga aatgaccaaa gattgccccg ctcgggccaa ggaagcgcca tagtccttcg
     5221 ttcccgagga ggaggtgtac atgggaatga gtggttgcgg aaatcccggc tcgtgcgtga
     5281 aaacttgcag ccactcctcg ccgaaagtgc ggcgccagac ccaggctgtc gcgaagctag
     5341 ttccaaatgg gatggccgtg aaaggctcgc gaaagatgtc attggcctgt ccgatggtga
     5401 ccacatcgcc gagttgtgtc gcttgcaaat ccaagctgac ggcggaagcc gtggcccaat
     5461 agtagttgaa cgccaaggtc agatggtctc cggtccagat caggcccgca gagcttgggt
     5521 aagccacgac gtctcgttcg agagtgatga agattgggtg tcgagtaccc atctcggctt
     5581 cgaaagagat cttcgccaag ctagagtcga atggaggaag tggatcgaca ggtggcggcg
     5641 gtcgcaccgt cgcctcgctg tacgcgacca ccggtccttc cggcgtcatc accaaagatt
     5701 tctgcaacca gggtttgtcc gaaacggaca aggttttgcc tgccgcgcaa ttgaatgcgc
     5761 gggtcgcggg ctgcgcccac tggcacgagc cgagcatcgt ctcatattcg aactcgatcg
     5821 caaaatccgt cggggcctgg gatgctccca cattcagcaa tgccgagtac ggatcgctcg
     5881 gttcgagatg ctccaaaacc gagcaattct caccgataca agatgcttgg atgttgttcg
     5941 cgttctgcgg aatcaacgcg tccacgcctg ccatcaagtc tgcggcgcgc actggattcg
     6001 tcgtgtagtc ttccgttggc gcacaatgga tgcaggggca cgtggccatg ttgcagaagc
     6061 cattacagct tgactcgccg cttgcgcacc actccgtatt gccagtcgcc gaaccttgat
     6121 tggcctgcac caggatcgga ccaggcacca cttcctgagt ggaggatgtg acccgaagcg
     6181 cctctcgcgt gacggtcaga gacattcccg tcgttggcgg tacgcgaata cgcaccgctc
     6241 cagtgcaagg ctccgtcgcg ggcggttccg aggtacttcc gccctggcca ccagcaccag
     6301 actcgccgcc aatcaccgtt cctccgctcc cgcctagggg agcgcctcca ccagcagtca
     6361 ttccaccagc agtcattcca ccagcagtca ttccaccagc agtcgctcct gcagcggatg
     6421 ttccgccgct ggctgttcct ccggattcga tggtcccgcc cagtcctgta tccccggcct
     6481 gcgtgaacga gcctccctgg gagctggagt tcgacgaccc gccctcggtc gtgccggccc
     6541 cggtgccccc gacgctcgtt tcccccgcgg ctccgccacc tgcggaatca tctcccccag
     6601 cggacgaggt ctgcattcca ccggtcgagg attgtccgcc gccgttcttc gttccgccgc
     6661 ctcccgagat ccggccgccg ctgccgcccg aagaagtatg gccgcctgaa cctggcgact
     6721 ccggactgct tgtcgagcag gccactgcca ggcccaccgc cgcaattgcg gaccaaagtg
     6781 gagttcgttc cagaaaccca tgtccaattg gaagccgccc tgagatccgc atgcaagact
     6841 tacccatcga tgttctctcc tgcctcctca atagttcgtc tagtgcgtct tcgcggctac
     6901 ggacgaatat ggacactctg aacttacttc gttctaactg agtgacgagt ctccaatgtt
     6961 ttcgatgaga cagacgtgaa atgagcgagt cgttccccag gccctcaacg agaggtcgat
     7021 tgagaagcgc gctccgctgt tcgccaatat atgccccgaa cgacacggcg aacaggcgcg
     7081 gagatcaatg ccatgttcga aactgataga aggcagccac gccagggggc gcacacgctt
     7141 cattgcatcg gcctacaagg cggacggcgc ccgagcactc agggttgagc gcaaatatcc
     7201 ggcctagtcg acgaacgaga cgtcgtcgat ccacgcgtcg aagggagtgt cttgagcgaa
     7261 taccgtgaat tcgacgctga atacgcggtg cggatcgaac tccaacggta agccgcgctc
     7321 catttgaagc aggtcgggcc agcgcgcgac atgataggtc cagtctgggg agagatcgac
     7381 ggcgacgcga tgtacgttgt tgcatccacg ttcgcacgtc ccgcccagac tgcgcggaac
     7441 cacgtccaag atcggcatcc ccaagtagat gcgacctcgc ccgcgtgtcc aaaagccgac
     7501 tccgccgtac gccgagacgt cgtagcactt ctccggcgcg agcaggctgc tgacggaggc
     7561 cccccagagc gtcaatctcg gccccgagaa gtgcagcgcg tattgactcg cggctcgggc
     7621 gagcatgcgg gttggatgca tgacgcggct cgaagagagc ggctccgtgc gcgtcctccg
     7681 gcctgtacca tcatcggcga atatccaagt gccagagcgg ccatcgagaa gctcgagttg
     7741 cgcatcgccg tcctcgaaag agtcaattga caatccgact ccacgcgggc tgctccgcgc
     7801 ggggcacgct atcaaaccca cggtgtgccc cggtttggcc gcgttatcgt cgcgcggggc
     7861 gggaacgaaa gcggcctctt tccggatcag tccagggccc cgctccgctc cgtccgagaa
     7921 ccagggaccc gagaagcgaa cggagagcaa cacgatccct accaccgctg cagcgaggag
     7981 gaccagccac gggctgcgat gactcgagcc tgggtgtgac gtctccaatc ggcgcaacgt
     8041 ggcgtccaag gcttggcggc gggaggcggc gcttggccgg tccaaagtgg cggagcggat
     8101 caatatccgc cgcagctccg cttcccgtaa ctcgcgggct tcacgggcct cgggttcagc
     8161 cggagtaccc tgcgacttcc cgcctccagg gctagcgtca cgtttcatgg gcgtgcgtgg
     8221 cccaggtttt cgcgctcgac caagcgcttg acggctgaat tgaagatctc gcgggcgcgg
     8281 tgcaggcgcg aattgacggt gccgactggg atccccaaca tcgtcgccac cgcgcctggc
     8341 gcttgatcct cgagatcgca caagacgaac accgcgcgtt gcgtgtcggt cattcggtcc
     8401 agaatgccct gcaggatggg gcgagcgtgc tggagttgcg caatctcttc cggatcctgg
     8461 gcaatgcacg gtgcgtgctc cagagttccg cccagctctt gcggccgtct tctacatcga
     8521 cgccgatagt tcgaggcaac tcggcccgcc gtgccgaaaa cgaacgcgcg ctcgcgaccg
     8581 ggcagaatgc tggcgcgttt gcgcgcgacg atgatccaaa cttcctgaac ggcatcctcc
     8641 agatccggct ctgggatacc gaaacgcctc agcgtgcgcc acgcgaagtc gaaatactct
     8701 tccacgaaag cgcgcagcgg gcaggaagac ccaccatctg ctaccgcgtc tgaaccctcg
     8761 aaggtctgga cggaacaggt gtgaagcggt ccctcgacca cgtactcagc cacgaccact
     8821 gcaatgtccc taggcccatc gtttcttcca actaaattgc tgcccggggt gagcggggca
     8881 cttggctagc ttcggcaccg aattctgagg ctcacttgcg tttaacgtaa cgaatatcgt
     8941 cgatccagac atcaaaccgc ggaacatcgg ggccgacgac gaagccgaga aataccaccc
     9001 ggtgcggatc gaactccagc ttcggtcccg cgcggagctg cgctaagtct ccccaaggca
     9061 ctgagtatga actccagcgt ggaccgagct ctatgcgctt cttgtagccg ttataacagt
     9121 ccttttggca aaagccgccc tcgtacggct ctacgatatc gaccgtccag accacgaggt
     9181 gtacggtgcc aaaccctttg gcccagaact cgagcccgtc ataagccgaa ctatcgtagc
     9241 aggcccgctc tgccaagaag ttcccgaaca gcgcgcccca ctttttgaag ccactgccgc
     9301 tcgtgtggag tgcccattgg ctgcgcccgc gcggtggtga aatgagagac ggaaagagca
     9361 ctcgatcggc gctcggcgtc tgagtgccgc tgccgtcgtt gtgaacgaac caaaaacccg
     9421 agcgaccgtc cgccagttga agccgcgagt tgccatcttc caagtcatcg atgagcggcg
     9481 catcaccctg cgcgaccacg acctttcgac acgcgggcag agacggcaca gagggcgcgt
     9541 cttgcgtccc cggctcccga ttggacatcg tcgcggtaga gaccggaccg gccgccgtgg
     9601 cgctcgcaac tgcagatccg agcgctacgg ccggatgctc gtc
//
LOCUS       DASTKU010000157        10265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_921073, whole genome shotgun sequence.
ACCESSION   DASTKU010000157 DASTKU010000000
VERSION     DASTKU010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10265
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921073"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFQ61_05610"
     CDS             complement(<1..232)
                     /locus_tag="VFQ61_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HET9953955.1"
                     /translation="MRKLGAGGMGSVWLAYDLSLDSTCALKLIEDDKARSGEVRMRFE
                     REAKAAAQIRGTHVVDVFAHGEWNGTLYIAMEY"
     gene            1695..2663
                     /locus_tag="VFQ61_05615"
     CDS             1695..2663
                     /locus_tag="VFQ61_05615"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HET9953956.1"
                     /translation="MSRAGFRAPPNRPRPAPHVPQAAGASSRASHATPNPSPFRPRRE
                     RRVEGHHYFEGFVRKSEHEEFLGWLHTLRPLWEYRYAGGNGASSNPGAGDRRLLRPVY
                     WLGNWQFACLDYYRPPHGVRDRCVQAEPFPPLLRHWVKDIEQRIQRLFPPAHIPPRFH
                     LNTCLVNFYGNRLEDGRWQDCARVGEHRDFEPGPVASISIGERALIQFVLPRPRGERA
                     EPLLSQWLDDASLQIFGGPLLKDRALHRVQRVDRRAKLELPPAIQGFSTRRVNLTFRY
                     VPDEHIVPFERLGEKPRGLVQGYVAELAQHSEFFKAALERVRARDR"
     gene            complement(2682..3227)
                     /locus_tag="VFQ61_05620"
     CDS             complement(2682..3227)
                     /locus_tag="VFQ61_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET9953957.1"
                     /translation="MSRLDNLRDQFRDPMRDIKLNVQSVLAEGGALNAAQRLGVALAS
                     AGAARDSELTEAILELCLAEAGQAVVDDALAASALMAMNNVYYRFRHLVGKAAYSERP
                     ARLRMTRIAKPATNKADFELFCLAVSAINACESCIRSHEEAVLAGGLTEEHVHDAVRI
                     ASTIHAAAVSLELSRLPSAAG"
     gene            complement(3559..3969)
                     /locus_tag="VFQ61_05625"
     CDS             complement(3559..3969)
                     /locus_tag="VFQ61_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET9953958.1"
                     /translation="MRHDEIIALLLRHGIQPSAQRVAVADYVLFTDEHPSADRVFARV
                     KKALPVLSRATVYNTLNLLVKKGLLRELVIAEGKVVFDPKLERHHHFLDESSGRIYDV
                     PWEALEVKKLGSLEEFEIDEYQVVLRGRRAVKRK"
     gene            4199..5389
                     /locus_tag="VFQ61_05630"
     CDS             4199..5389
                     /locus_tag="VFQ61_05630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9953959.1"
                     /translation="MSPLSLLFVALFNSILGLSVLFPILGPLGRQLGLTELQIGALTT
                     AYALAQFAGSPFWGKRSEVVGRKPVLLRGILGFALTFAAFGVIARLGQLGVLRGNGLF
                     AALLVSRLMGGLYSSATLPTVQAYVADVTERDHRTSGMALIGAAFGLAVVFGPAIGAL
                     LAPFGLLVPVFVSASIALLNALFVAWKLPEPVRHERSPVAPELLPVASKVWVLLGVGF
                     VATVAAVSMEQTVAFYFQDRLALNETQAARNVGFSLVAYGVVAVLVQGVFVRRAKWPP
                     LRLLRLGLPIAAFGLFTFVLARGLPLLLLAMVLQGLGQAMTSPGVTAALSLGVQDEEQ
                     GAVAGLNSASQALGRTLGPVLGTALYEIRPEYPYLFGCALLVVMSLLLFGGHIQRSRA
                     AAPR"
     gene            5611..7158
                     /locus_tag="VFQ61_05635"
     CDS             5611..7158
                     /locus_tag="VFQ61_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953960.1"
                     /translation="MAPSSPEKKKEAAAPQVPPPPDICQSYVRNGGLCPPSAAFEASL
                     ADALELQDAGQRDAALSCLEQGSVQQGSVQQGSPQPNRSDTAALIRALRADLAPTACA
                     DVLVSEFLASSKQKLKPDEESALLGLLVAGKLSRLSNGAPALTPPFDKPHFEDFFEHQ
                     LKPWLLAQAIAIGSLSRDGARLTGYGKAIAAVEAGLADLRFVQVARQVELPKELAADP
                     DVKNVYYAALDEALEPRKTRGRDAALVGLRLLAELGVLADPRVQRARELLAASYGGSR
                     IDALDRLLLPDMPALKLDTPLTRLRARLPPFYAQRLLPTNAEPLDASVLRALGERGIP
                     APVRAQLDAELASLPADVRRIYAALELRRGSLYFSGAAFAHAAQLAAMNAGAADAEAR
                     FIEALARALEEAPKDAVELMLGSPRLPRLGNSEPLRKLASQAGPYRAQAEFDAAYLDY
                     LAPGTEQTARFWRELAQRFERAARRLSAGPAQKYAQDLARSAAATADSLASTPPVTHG
                     TPKAAVP"
     gene            7379..9082
                     /locus_tag="VFQ61_05640"
     CDS             7379..9082
                     /locus_tag="VFQ61_05640"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HET9953961.1"
                     /translation="MSEFSYQELLPIAHGDSEGHDSENHGAGDESGHEATPYRRLDID
                     AVSTFEAQGRTFVKVEPEALTALTREAMRDIAHLLRPGHLAQLRKIYDDPEASPNDKF
                     VALELLKNANIAAGFVLPSCQDTGTAIVMGKKGQQIFTGGNDEAAIARGVYDVYQTSN
                     LRYSQLAPLDMYREVNTSTNLPAQIELYACEGDEYRFLFMAKGGGSANKTYLYQETKA
                     LLNPQSLLTFLREKLKTLGTAACPPYHLAVVIGGTSAEFALKTAKLASARYLDTLPTS
                     GNALGHGFRDLELEAQVLKITQESGIGAQFGGKYFCHDVRVVRLPRHGASCPVAIAVS
                     CSADRQAVGKINKHGVFLEALERDPAAYLPDVSGGLDTSVVKLDLNRPMAELRAELSR
                     YPIRTRLSLSGPMIVARDIAHARIKERLDSGQPMPDYMKNYMVYYAGPAKTPEGYASG
                     SFGPTTAGRMDSYVEAFQSQGGSFVMLAKGNRSPKVTEACKKYGGFYLGSIGGPAARL
                     AQDCIRKVEVLEYPELGMEAVWKIEVEDFPAFIVVDDKGNDFFAEILAGKAPTGLPVV
                     K"
     gene            9132..9638
                     /locus_tag="VFQ61_05645"
     CDS             9132..9638
                     /locus_tag="VFQ61_05645"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET9953962.1"
                     /translation="MSALKSHIVEIPDFPKPGILFRDITPLLRHHFEATMCELEAALS
                     PKEWDQVDAIAGVESRGFILGAGLAARNRKAFVPVRKQGKLPPPVVDVSYDLEYGTGK
                     LEVQKGSGRLLLVDDVLATGGTLRASADLCTTAGYSVTGLLVLVDLRLRPEFVWGSLR
                     AASIVSYG"
     gene            9631..10206
                     /locus_tag="VFQ61_05650"
     CDS             9631..10206
                     /locus_tag="VFQ61_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953963.1"
                     /translation="MADWLVVMALELEAQGRFAAAGVDVLFTGLGKVNAAHRLTRRLV
                     ELRARGQTPGVVNFGTAGSRVHRTGSIVQCRRFIQRDMDVTGLGFALGETPFEAVPRE
                     LTFDPVFSDLPDALCATADRFETLDHAADLEVIDMEAYALAKVCWLEGVTFACAKFIS
                     DGADQHAGTSWEQSLPHASRAFLELYERLPR"
BASE COUNT         1921 a         3215 c         3154 g         1975 t
ORIGIN      
        1 ggtactccat ggcgatgtag agcgtcccat tccactcgcc gtgagcgaac acgtccacga
       61 cgtgcgtccc ccgaatctgg gcggcggctt tggcctcccg ttcgaagcgc atgcggactt
      121 cgccactacg cgccttgtca tcctcgatca gcttcagagc acaggtagag tcgagcgaaa
      181 ggtcataggc cagccacacg gagcccatgc caccggcacc cagcttgcgc actaatcgat
      241 agcgctcagc gattacgtgg ccaaggtggc gttccaaacg ttccttcccc gcccggccgg
      301 cgtgacacac gtgcctgcgg cagcgccgca aacgcagttc cctgaatcaa ccgacaagta
      361 ttccaagcaa ttgagaaacg gcgttgggcc gtaagcgcag gtttcggctt ccgcctcacc
      421 ctgcaacgag tccgtagccc acggctcctc gttcggcaag ctttgcgccg ccgcctgaaa
      481 gcccctgctc ccccggtctg aatgctccat ctcgacgccg gccctctgca cccctctgcc
      541 gcaggcctgg tcaccacgtg aagagcgacg cttcgacagc ccagggcagc ttgttccgcg
      601 atctaccctc tcggagttcc gcgcgtgcag tacccgagaa ttcgtgcccc ctcgaaaaag
      661 ggagagctcg ggtgagctga ggctgaggtg ggaaatattg ctcatctaca ggattgcagc
      721 agcaatcatt gaagccgact acgtcgaggt ggtgatggcg gaggcagcag gaactgttgc
      781 catagaccga ggagactgag gcagagtggc cgggcttccc tgggatcgag aacccggaga
      841 cgggcgcgag ggggagcgcc cgctgagagg ggaccattga tagagtgggc gcatggcgca
      901 gacgaggggt gtctcagcaa caaggagcca agggacgagg ttgcgagaca gtgcaatctt
      961 acgagaccgt aaattcgcgg ggatgaaact caatgcacca ttcccgacct tgctgaacgt
     1021 aaccgttggg gctgtgttgt tagtcgtctg gagtggtaag cacggctcgc aattctttgc
     1081 gcgaggtgcg actctcaatt gtacctgagt ttgcgtcgga gaacgcaaca tcctcgagga
     1141 ttttctaagt tctcaggatc agaccgatcc cttgattggg cggcgcaaga cacaatgatg
     1201 tgcgcccgga gcggcgggtc acttgggatc ggtgggggcc gcgtagttgg gcgagaccgc
     1261 taagttcggc ggccgtgttc tctaaacggc cgtgttctct aagcggccgt gtaacgagcg
     1321 caggcacgcg gatcatgcaa cgtaaccgga gccgcaatcg agcagtgtcg ccaacatagc
     1381 caacacacgc gccggcaaag agctgtttgc atcccagctg tttgcatccc agctgtttgc
     1441 atcccagctg agtgggtatg tcaggccaga accgaaacga tttgccaaac gcaagtttgc
     1501 gcccaaagcg gcggcgccct gtgccgccgt cgatccaaag cgcctccacg gtatgccccg
     1561 gcaaaccgaa ccccaagccg gcctaaagca gagcacaatt ctcggccatc ccacacctcg
     1621 agctagtcct aacccccgga gtgatccatc actctacccc cgcggccgca tgtgacgtat
     1681 gggggccacg atcgatgtct cgcgcaggtt tccgcgctcc acctaaccgg cctcgtccgg
     1741 ctcctcatgt cccccaagcg gcaggcgctt cctcgcgtgc cagccatgcc acgccgaatc
     1801 cctcgccgtt tcggccccgc cgagagcgcc gcgtcgaagg gcaccattac ttcgaaggct
     1861 tcgtgcggaa gagcgagcac gaagagtttc tcggctggct ccatacgcta cggccattgt
     1921 gggaatatcg ctacgcaggg ggaaacgggg cttctagcaa tccaggggcg ggtgatcgac
     1981 ggcttctgcg ccccgtctac tggctcggga attggcagtt tgcctgcctc gactactacc
     2041 gacctccaca cggggtgcgg gatcgctgcg ttcaagctga gcccttcccg cctctgctgc
     2101 ggcattgggt gaaggatatc gagcaacgga ttcaaaggct attcccgcct gcacacatcc
     2161 ccccgcgctt ccacctcaac acctgcctcg tcaattttta cggcaaccgt ctagaagacg
     2221 ggcgctggca agactgtgcg cgtgtgggtg aacatcgcga tttcgagccg ggccccgtag
     2281 cctccatctc aatcggagaa cgcgcactca ttcagttcgt gctcccgaga ccccgaggtg
     2341 aacgcgctga gccgctgctc tcgcagtggt tggacgacgc atcgctgcaa atcttcggcg
     2401 gaccactatt gaaagatcgc gcactccacc gcgtgcagcg agtggaccgc cgcgcaaagc
     2461 tcgagctgcc ccccgccatt caaggattct cgactcgacg tgtcaacctg acctttcgat
     2521 atgttccgga cgaacacatc gtgcccttcg aacggctcgg cgaaaagcca cgtggcctcg
     2581 tccaaggata cgtggccgaa ctcgcccagc actccgagtt tttcaaagcg gcgctagagc
     2641 gagttcgagc cagagatcgt tgaaccagtt cgcgtagacc ctcaacccgc agcgctgggc
     2701 agtcgagata gctctagaga tactgcggcc gcgtggatcg tagacgcaat gcgaacggcg
     2761 tcgtgtacgt gctcctcggt caatcctcct gccaacaccg cctcctcgtg cgagcggatg
     2821 cacgactcac aggcattgat ggcactcacg gccaaacaga acaactcgaa gtctgctttg
     2881 ttggtggcgg gtttggcgat gcgggtcatg cgcaggcgcg ctggtcgctc cgaataagcg
     2941 gccttcccca ctagatgccg aaaccggtag tacacgttgt tcatcgccat caaagcggag
     3001 gccgctagcg catcgtccac gacggcctga ccggcttcag ccaggcacaa ttcgaggatc
     3061 gcctctgtca gttcggaatc acgtgccgca cccgcagaag cgagcgccac gcccaaacgt
     3121 tgggccgcat tcagcgctcc accctcggcc aaaaccgatt gtacgttgag cttgatgtcg
     3181 cgcatcggat cgcgaaactg gtcacgcaaa ttgtcgagcc gggacataga atgaacctcc
     3241 agggggtgag tacagtccag atttagacag agtctaagtc agcctaacga tcacggcaag
     3301 gcttctgtag agccaaagat gcgccgcgca tgacgccgct cgctacgagg ctcgcgccaa
     3361 ggtgacgcga caccgccttc tccgcaccca gcagagacct gagggtactc ggtccgagaa
     3421 agagagttgc gagccctaag gtgacgcgcc tccgaatgag tcaccgctcg acgctacgaa
     3481 aacgggctcc agaggccgct tcgttcgagg aagcgggagc acgtccgtgc cggagcggcg
     3541 tcgggacccg aatcgccctt acttgcgttt cacagcgcgg cggccgcgca gcaccacctg
     3601 gtactcgtcg atctcgaact cttcgaggga gcccaacttt tttacttcga gagcttccca
     3661 gggcacgtcg tagattcgcc cggaagactc gtccaaaaag tggtggtgtc gctcgagctt
     3721 cggatcgaat accaccttac cctcggcaat cacgagctcg cgcaggaggc ccttcttcac
     3781 gagcaggttc agcgtgttgt aaacggtggc gcgagacagc accggcagcg ccttttttac
     3841 tcgcgcaaac acccggtccg ccgatgggtg ctcgtccgta aacagcacgt aatctgctac
     3901 cgccacgcgc tgggcagacg gctgaatgcc gtggcggaga agaagagcaa tgatttcgtc
     3961 gtgccgcacc ggtttagaat gagtctagat cgggaatcgg tcaagcgact aaaggccgat
     4021 ctgtgagcac tttcgcgcac gtggtatgcg ggagcctccc ctggtggtcg acgcactgcg
     4081 gccactctgc ggggtgctag tttcccaccc acgctcgcct atcccgtccc gattacgaac
     4141 ttggtgcccg ctcctctcgg atttgagccc tgccgtcccc cggaaatcaa gccacgttat
     4201 gtcaccgctg agtctactgt tcgttgcgct cttcaacagc atcctagggc tcagcgttct
     4261 atttccgatc ttgggtccac tcgggcgtca gctggggctg acggagctgc aaattggggc
     4321 gctcaccacg gcttatgcac tcgctcagtt cgcgggcagc ccattttggg gcaaacgcag
     4381 cgaggtggtt gggcgcaaac cggtattgct gcggggcatc ctcggcttcg cgctcacctt
     4441 cgcggcattc ggggtgatcg cgcgtttggg tcaactgggc gtgcttcggg gtaacgggct
     4501 cttcgcggca ttactggtgt cacggctgat gggcgggctg tactcgtcgg ccacgctgcc
     4561 gaccgttcag gcctacgtag ccgacgtcac ggagcgcgat caccgaacct cgggcatggc
     4621 gctgatcggc gcggctttcg gactcgccgt ggtgtttggt ccagccatcg gcgcgctgct
     4681 cgcgcctttt ggcctgttgg ttccagtttt cgtctccgcc agcatcgcgc tcctgaatgc
     4741 gctgttcgtt gcgtggaagc tgcccgagcc agttcgacac gaacgttcgc cggttgctcc
     4801 tgagctcctg cccgttgcaa gtaaggtttg ggtcctgctt ggggttggct tcgtcgcgac
     4861 cgtggccgcc gtctcgatgg agcaaacggt cgccttctat tttcaggacc gcttggcgct
     4921 caacgagacg caggcggcac gcaacgtagg cttctccctg gtagcgtacg gagtagtggc
     4981 cgtgctggtg caaggcgtgt tcgtgcggcg cgccaagtgg cccccactgc gcctgctccg
     5041 gctcggcttg cccattgccg cgtttggctt gttcaccttc gtgctcgcgc ggggattacc
     5101 gctcctgctc ttagccatgg tgttgcaagg tctgggacaa gcgatgactt cgccgggggt
     5161 cacggcggcg ttatcgctgg gagtgcaaga cgaagaacaa ggcgccgtgg caggcttgaa
     5221 cagcgcctct caggccctcg gtcgtacgct cggccccgta ctcggcaccg cgctctacga
     5281 gatccgtccc gaatatccgt acctgttcgg ctgcgcacta ttggtcgtga tgtcgttgct
     5341 gttgtttggc ggtcacatcc aacgctcgcg cgctgcggct ccgcgctgag cgggcgttag
     5401 tcaacgaatt cagctgcgcg acttcactta ttcgtggcgc cgcacggcgt gcgccttcct
     5461 ggtgcgagcg gacccttgat tgggttagcc tagcgcccca tgcgacgcat tctcgctctg
     5521 tgttggttga cgagcgcctg cgcagcggct cccgctgtgc cgcccgccgc atctccggca
     5581 aatgcaccgg ccacgtcgag cgctccagtg atggcgccga gcagccccga aaaaaagaaa
     5641 gaagccgccg cgccgcaggt gccgccgccg cctgatattt gccaaagtta cgtgcggaac
     5701 ggaggactct gtcccccatc agccgcgttc gaggccagcc tcgccgacgc gctcgagctc
     5761 caagatgcag ggcagcgaga cgccgcactc agctgcctcg agcaggggtc tgttcaacag
     5821 gggtctgttc aacaggggtc accacaaccg aaccgctccg atacagccgc tttgattcgc
     5881 gcgctgcggg cggatctggc accgactgcg tgcgcggacg tgctcgtaag cgaatttttg
     5941 gcgagctcca agcagaagct gaaacccgat gaagagtctg cattgctggg cttgttggtt
     6001 gcgggcaagc tgagccgcct gtccaacggc gcgcccgcgc tcacgccccc tttcgacaag
     6061 cctcacttcg aggacttctt cgagcatcag ctcaaaccgt ggctgctcgc tcaagctatt
     6121 gcaatcggca gtctgtcgcg cgacggcgcg cgtttgaccg gatatggcaa ggccatcgcg
     6181 gccgtggaag cggggcttgc agacttgcgc ttcgtgcaag tagcgagaca agtcgagcta
     6241 cccaaggagc tggccgccga tccggacgtc aagaacgtct attacgcggc gctcgatgaa
     6301 gcgctcgaac ctcgcaagac acgtggtcgc gacgccgcgt tggtgggcct gcgcttgctc
     6361 gccgagctcg gggttctggc cgatccgcgc gtgcagcgtg cacgtgagct gttggccgca
     6421 tcgtatggcg gcagccgcat cgacgcactc gatcggctgc tgttaccgga tatgcccgcg
     6481 ctcaagctgg ataccccgct cactcggctg cgagctcgcc tgcctccgtt ttacgcgcaa
     6541 cgcctgctgc cgaccaacgc ggagcccctg gatgcgagcg tgctacgggc cctaggcgaa
     6601 cgcgggatcc ctgcaccggt tcgggcgcaa ctcgatgccg aactagcaag cttgccagca
     6661 gacgtgcgcc gcatctacgc ggctctggag ctgagacgcg gcagcctcta tttttcaggg
     6721 gctgcgttcg cccacgcggc tcagctggca gccatgaacg cgggagcggc cgatgccgag
     6781 gcgcgcttca tcgaggcctt ggctcgtgcg ctcgaggaag ctcccaagga cgcggtcgag
     6841 ttgatgctgg ggtcgccgcg cctgccgcga ctcggaaact cggagcctct tcgtaagctc
     6901 gcgagccagg cggggccgta tcgcgcccaa gccgaattcg acgccgcata cttggattac
     6961 ctcgcacccg gtaccgagca aactgcgcgc ttctggcgag aactagcgca acgcttcgaa
     7021 cgggccgcgc gacgcctcag cgcgggtcct gcccaaaagt acgctcaaga tttggcgcgc
     7081 agcgcggcgg caaccgccga ttcgctcgcg agcacaccgc ccgtaactca tggcacgcca
     7141 aaggcagctg tgccctgagc ggcatcagcc gagtgacgaa ggcatgggag catcgtcctt
     7201 cttgagttac cctgcgctcg cgtgcggccg agcgaagtat tacgggctgc gttggtcctg
     7261 ggctcggcgc tttccagatc tcccctcgaa agcttgagct aagacagatt tcgttcgcgg
     7321 cccgggcctg cccggttcta aaacctgctc accagtaatc gattcagacg gaaacaagat
     7381 gtcggaattc tcatatcaag agctgctccc catcgcccac ggcgattccg aagggcatga
     7441 ctccgaaaac cacggagcag gggacgaatc aggccacgag gctacgccgt atcgtcgtct
     7501 cgacattgac gcagtctcga cgttcgaggc gcaggggcgg accttcgtca aggtcgagcc
     7561 ggaagctttg acggcgctca cgcgcgaagc catgcgcgac atcgcacatc tgctgcgtcc
     7621 tgggcacctg gctcaactgc ggaagatcta cgatgatccg gaggcttcac cgaacgacaa
     7681 attcgtcgcg ctcgagttgc tcaaaaacgc aaatattgcc gctggtttcg tgctcccgtc
     7741 ttgccaagat accggcaccg ccatcgtgat gggcaagaag gggcagcaga tcttcaccgg
     7801 tgggaacgac gaggctgcga tcgcgcgcgg agtctacgac gtctatcaga cgtcgaacct
     7861 gcgttactcc cagctggccc cgctggacat gtaccgcgag gtgaacacca gcacgaatct
     7921 gcccgcgcag atcgagttgt atgcctgtga gggagatgaa taccgcttct tgttcatggc
     7981 caaagggggc gggtctgcga acaagactta cttgtatcaa gagaccaaag cactgctgaa
     8041 tcctcaaagc ctgctcacgt tcttgagaga aaagctgaag accctcggca ccgcggcttg
     8101 cccgccgtac cacttggcgg tcgtgatcgg tggaacctcg gcggagttcg cactcaagac
     8161 cgcaaagctt gcgtcagcac gttacttgga cacgctgccg accagtggca acgcgctcgg
     8221 gcacggtttc cgagatctcg agctcgaagc gcaggtgcta aagattacgc aagaatcggg
     8281 cattggcgct caattcggcg gcaaatactt ctgccacgac gtgcgtgtcg tacgcttgcc
     8341 acggcacggt gcgagctgcc cggtggcgat cgccgtatcg tgttccgcgg atcgtcaggc
     8401 cgtcggcaag atcaacaagc acggtgtgtt cttggaggcg ctggagcgcg atccagccgc
     8461 gtacttaccg gatgtgagcg gtggtctcga tacctcggtg gtcaaactgg acttgaatcg
     8521 gccaatggcc gagctccgcg cagaactttc gcgatatcca attcgcacgc gcctgtcgtt
     8581 gagcggcccc atgatcgtgg cgcgtgacat cgcgcatgca cgcatcaaag aaaggctcga
     8641 ctccgggcag cccatgccgg attacatgaa gaactacatg gtctactacg ccggcccagc
     8701 caaaacccct gagggttatg cttccggcag cttcggtccg accacggccg ggcgcatgga
     8761 tagctacgta gaggcttttc aaagccaggg cggtagtttc gtcatgttgg cgaagggcaa
     8821 tcgttcaccc aaggtcaccg aagcgtgcaa gaagtacggt ggcttctacc tcggttcgat
     8881 cggaggcccg gcggctcgcc tggcgcagga ctgcatccgc aaggtcgaag ttctggagta
     8941 cccggagctc ggcatggagg cagtctggaa gatcgaggtc gaggacttcc ccgcgttcat
     9001 cgtggtggac gacaagggca acgatttctt cgcggagatt ctggccggca aagcccccac
     9061 tggtttgccg gtcgtcaagt aggtggcacc gcaaggcgcc ccgaacgcgg aaaggcacac
     9121 ggcacaacaa catgtcagcg ctcaaatcgc acatcgtcga gattcccgac tttcccaagc
     9181 ccggcattct gttccgagac atcacgcctc tgctgcggca ccacttcgag gccacgatgt
     9241 gcgaactcga agcagcgctg agtccgaagg aatgggatca ggtcgacgca atcgctggcg
     9301 tcgaatcgcg tggcttcatc ttgggtgcgg gcttggccgc ccgaaatcgc aaggcgttcg
     9361 tgccggtgcg taaacaaggt aagttgcccc cacccgtcgt ggatgtttcc tacgatctcg
     9421 agtacggcac cggcaagcta gaggtccaaa agggctccgg ccgcttgttg ttggtggacg
     9481 acgtgctcgc aacgggcggc acgctgcgcg cttctgccga tctgtgcacg accgcgggct
     9541 actcggtcac tggtttgttg gtactcgtgg acttgcggct gcgccctgaa ttcgtttggg
     9601 gttcgctgcg ggccgcgagt atcgtcagct atggctgatt ggctggtggt gatggcgctc
     9661 gagctggaag cgcaagggcg cttcgcggcg gcgggcgtgg atgtgttgtt cacggggctc
     9721 ggcaaggtca acgcggctca tcgcctgact cgccgtctgg tcgagctccg tgcacgcggg
     9781 cagactcccg gcgtcgtgaa tttcgggacg gcggggagtc gcgtccaccg caccggctcg
     9841 atcgtgcagt gccgtcgctt cattcagcgg gatatggacg tgacggggct cgggttcgca
     9901 ctcggagaaa ctccgttcga agccgtgccc cgtgagctga ctttcgatcc ggtgttctcc
     9961 gatttgccgg atgcgctgtg cgcgactgcc gatcgattcg agacgctcga tcacgccgca
    10021 gatctggaag tcatcgatat ggaagcctac gcacttgcca aggtctgttg gttggaaggg
    10081 gtcacgttcg cgtgcgcgaa gttcatcagc gatggtgcag accaacacgc cggaaccagc
    10141 tgggaacaaa gcctgcccca cgcctcgcgg gcgttcctag agctgtacga acggcttccg
    10201 cgatagcgtc tccgcgtctg cgtggacccc tcgcgtgagc tcactcgcga cggggtcacc
    10261 gaggt
//
LOCUS       DASTKU010000158        18535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_476310, whole genome shotgun sequence.
ACCESSION   DASTKU010000158 DASTKU010000000
VERSION     DASTKU010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 18535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18535
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476310"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            538..2460
                     /locus_tag="VFQ61_05655"
     CDS             538..2460
                     /locus_tag="VFQ61_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953964.1"
                     /translation="MLKLSSAWVLGWVAMGLVGVACTVVTDGDDDHTGGSSSSGGRGS
                     GGRSSGGTTSSAEGGMAEAGETSTTGGRASTGGSSSGGRTSTGGRSSGGTTSSGEAGA
                     GGGTDTPPVELFDCDLRSTTGAQLIDEDVSTNTTWSGTINIRRNLRVVEGATLTIEPG
                     THIVMGPDSMIEFGWNTNAATVKAVGTAEKPIRFCGEQGDPGFWSGISVGSNVTSNSQ
                     LSHVLISDAGADEAALTLASNIEITDVQIRNSEKDGVHAVDFSEGSAQLSVEGAQGTA
                     VVATHADAIDRFPLGGTFSNNQNNLVALDFGSIETDVTFADVGIPYQHEGTLQIRKAK
                     VVIDPGVTILMGADAFLDVGWNTNAGTLTAVGTPEAPIVFRGVEEQSGFWAGILIETN
                     VTTNSSLSNLEIWHAGGNDQYALEVRSPINIDDVRLSDNDQGAFIGADGVSDDSKNLS
                     ITRTKKVALTVEPNGLIALPQGGDFTGNDTDEVQIQGGNFEVSGTIPNLGVPYRVLGD
                     ISTRKASMTLTAGTKFIMSADSGIEWGWNGNEATIIAVGTAAKPIQFVGADPVSGFWK
                     GLTVGTNALSSSKFDYVEVSHGSTACLSLNSSVPVTHSSFSQCEWGILHKTGDARDYA
                     ATNTFTQTSKGTVGTL"
     gene            2571..3626
                     /locus_tag="VFQ61_05660"
     CDS             2571..3626
                     /locus_tag="VFQ61_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953965.1"
                     /translation="MIVHRRSTAIGAALSLALWATSSQVRAQTNPSTERRPTDPAAAR
                     AQLKQGYDLKQQGQCEAAILHFEESARLDPQPKAFLNLADCEEKLGRLVAAQTHLVEA
                     RDLASSPAAAPLRNIAEERLRTLEARLPKLALRLASNAPEATRVWRDDVELGAVSLNV
                     ALPLDPGAHRVVVQHQGARRDYSVVLAEAESRELEVTPFADTPPAPASGQVTSKRSSE
                     SVPQKRLGADTSSGLPILPIALTGVAVAGVVVGAVSLVSLQDKNDEIDRVCPNTNCPD
                     QATYAKYEELNDSAKTARLFMIGGFAAGALAGGASAYFWFRASQASQNGTTARPQFAV
                     QPILIPGSPGLLVRGGF"
     gene            3626..4738
                     /locus_tag="VFQ61_05665"
     CDS             3626..4738
                     /locus_tag="VFQ61_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HET9953966.1"
                     /translation="MHRRSQQHRLTQAWRRPGLLIWAALGISTLRCGVSERLPPGPVD
                     EPGGERGDPDKGGEYPGGAEGSEGGAKPTHGGATAGGMVNSEAGAKHEAGSGGTLEPI
                     ETTAGAGAGAQSDEGGAAGNAAGGGTTGNPTSQLGGTSGGSSVNPASGGASGTQSTTG
                     DGGSSGGATASGGQASGGRTGSGGTLNTGGKNLGGDDEPRAGRPACFVEGTPVTLANG
                     TQRAIEEIRVGDEVLSYDPEQQTFLRGQVVQLFVHPHTPALVEIDHRVTTTPEHPFFA
                     SGRFKRADALNLDEDRLFEWIQRPGTQPLFGDVGANPSRDASTIFLSAAREHELESLV
                     TRAADVTTYNLEVVPSHTYFAAGFLVHNLKPIPEIP"
     gene            4952..6247
                     /locus_tag="VFQ61_05670"
     CDS             4952..6247
                     /locus_tag="VFQ61_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953967.1"
                     /translation="MAFSWRQDWRRGSSAAVLVLLIGCSSDKSTTPSTGGTPGSNGGQ
                     GGSIAGTSSGGMSTATGGTTASTLGGNTQGGASGGSTPTTSGGTSATGGTSVSAGASS
                     GGSTQAGASQGGGGAQAGGGAGGTSGASAGGATSAGSGGGGTPVELKIPIERSGKYVL
                     EFGDVTFSVDPMIGARIASFALGGKSTITGQDTDTKGEGNWGSTYWPSPQSTWGWPPI
                     PELDKNPYTPSVTGNTVTMVSAVAAGTNKLSLTKKFTPVLDAQAVDVEYVLKNEASQA
                     VSWAPWEISRVPSGGLAFFPTGTKVVPVTSTDPLPTAKIVNQAGVSWYQSSAADKGKY
                     NADGAEGWLAYASGDLLYIKRFEDIPADKAAPGEADVEIYAGGAYVELEPQGAYQSMP
                     AQGTLSWKVRWYVRKLSDTSIVKLGNADLLALVREVIKK"
     gene            6308..7762
                     /locus_tag="VFQ61_05675"
     CDS             6308..7762
                     /locus_tag="VFQ61_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953968.1"
                     /translation="MLVWSSVERLTVGIEELERQRLWDAAQVPWEVLDYERWIAQVSA
                     TPTDDYLFSSAKARFEAADSDRLLALVDVEIQGPASAPKLYSKSLAVSLSLSGLSRQN
                     AEQCLLALRSNRTVAELRSELPSMDAFLTQTLGLLVFAPEALTELEARVPGTELVRFV
                     GTPYEIVRAYWENAADVRVLAEDILSGVRDGASFLESLRALHVVTLMGRRLDRFYCPA
                     SKVARAGVRPGMLFAASTALQSTARDLLLLSGPRVGAPYVGGARYHELLTWGLVARAD
                     RANSSATASCPPQPSRHDQSGHVSDWGRVIRARAPHEASVESWFIPPRPMTSSHFDAL
                     WSAYAEATQASRRADTPGLYDALARFHYRFARLHPFRCANQSLSMNLVNALLQSTGGG
                     IPHLLLDHVAFHVDEPAYACVFQRAVKHHRLITGSAPPNPSQRWQFLRQRKQQSYALV
                     EKIQKANSLEHAKQICKADPTGSEAALIDLNALA"
     gene            complement(7877..8842)
                     /locus_tag="VFQ61_05680"
     CDS             complement(7877..8842)
                     /locus_tag="VFQ61_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HET9953969.1"
                     /translation="MGTVRDFIQHHFRHFNAAALVDAARGWEAHLAQGGKMFLTMGGA
                     MSTAELGIVIAELIRQDKVHAISLTGANLEEDVFNLVAHESYRRVPNFRELRPEDEKA
                     LLDKGLNRVTDTCIPEHEAMRKIEKKMLALWERAQKKGERYLPHEYFYQLLLSKELES
                     EYEIDPKHSWLLAAAEKNLPLFVPGWEDSTLGNMFVAGVIRGEYGYQIMKGGTEYMGR
                     LIEWYKEVDQQHSIGFFQIGGGISGDFPICVVPLMDQDLGWSVKTWGYFCQISDATTS
                     FGSYSGAPPGEKITWGKLNVDTPRYVIESDATIVFPLIAAYLLGM"
     gene            9731..11686
                     /locus_tag="VFQ61_05685"
     CDS             9731..11686
                     /locus_tag="VFQ61_05685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9953970.1"
                     /translation="MMDRYSTPPPATLSGMVVYSEVQPGVSYRLERQIGEGGTGMAYL
                     ALRQAQDGLSPVVLKLVHTDPTSEPAADLFVQKEAVALGRLNERVPPTPFVVRFIDTG
                     TTRVLDDTPMPWIAIEYVHGGIEGTTLEDRVLYSIHQTGCAFDPPRALHVVRCLAQGL
                     GAIHAVGVIHRDITPGNILCCGFGEAEIFKISDFGVARPQGLTATFGGLGVGTAGYAA
                     PEQSLPDHTVVGPHTDVFGLACVVYYVLAGEHLFDAQSPVQAYSMIKDKARRSICESR
                     TLSPEFTEQSDVCRAIDGAIARATSFAADQRPASVQEFAANIIPWLGAPSSAPRPSRR
                     LVHSVLQLTPRGDLSGWSWTVRHPPGDNVVIQSAAWDADGRCFAFTPRGPLFWNGQSW
                     LLAPEASRSLPEGMGFAKRYEAGGWLVGGAGGVLAIYGTEGVREVIHAPDTKTRFLHA
                     SGRFDDLLVAVAQRPSETPALWAMTARRWMKPLHLDGVAYVSGLHRLDDTRWVVCGRM
                     TEGVGFVAIYAPLDWEVTFLTTPHTRAFVAGASEPERNLALLVGASGVTVRLDGETAM
                     SAVAEGEPDLTASAMDVLAREWVASVSTLWMRDPEHQDWRTVWTSPEFRTPFISLMAD
                     AGLVVAMTVDGGIVEGRAAWRGRRPRR"
     gene            complement(12211..13110)
                     /locus_tag="VFQ61_05690"
     CDS             complement(12211..13110)
                     /locus_tag="VFQ61_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953971.1"
                     /translation="MTLPRQILPGRTYFVTRRCTQRMFLLRPSRATNLLISYCLALAA
                     YKTGVLLHAVCFMSNHWHGVLSDPFARLPEFLERFHRLFAKAQNAALGRRENLWSSDK
                     PSVLMLGSDQDILEKMAYTLSNPTLAGLVHSPEEWPGVITRFIDERRTVSIPDDLDDI
                     EDDMPESLELCFSRPPVLLQLSDAEVARQLNDLVSGYVRKARAELESEGRKFRGREFV
                     LRQSVESMPDTAPTSRPFVPRIVAKNQLIRKSMQAQFRHFVAAYRAARDLWRRGARDV
                     IFPAGTYALRIFAGVRCVPTVPS"
     gene            complement(13159..13935)
                     /locus_tag="VFQ61_05695"
     CDS             complement(13159..13935)
                     /locus_tag="VFQ61_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953972.1"
                     /translation="MARLPPAFRTIVDGRDLIESLSDLFGSKGSWILATGYVEQAEVR
                     LPTDGADTRRLFRGRYALSQFAGPLGGPYGATLSRVDSSGAVDLVSGILVSARSAGVH
                     ALCLSGDGEVTLAQSTGGTSAPAAAPIPTNAPVPGIASTNASVPGMSSNASVPGMSSK
                     AAEGGVQVAAGWAAQVNAVAAAQAVPEEEPEPAEPERGDLVQHFAFGLCEVLTANDDK
                     LMIRDLGGPGRIREIRTAMLNVERPTERDGKRLFRLTRKG"
     gene            complement(13965..14783)
                     /locus_tag="VFQ61_05700"
     CDS             complement(13965..14783)
                     /locus_tag="VFQ61_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HET9953973.1"
                     /translation="MANWRGVAGGDINEAYRCELDDGSLCFLKANPTARPEMFEVEAL
                     GLKWLREAGALRVPEVLACSRAGDTSSFLMLEWLEPGRPSRDFDDVLGRGLAKLHAAA
                     APEFGLDHDNFIGSLPQENHPTATWAEFFWSRRLEPQLRRAVSLGRVSLLMRRGFQEL
                     ERRIPRLLGEVKSSSRLHGDLWSGNVHVDDRGEPCLIDPAAYGGHREVDLAMLRLFGG
                     TSQRVFDAYAEALPLETGAQERVGLYQLYPLLVHVNLFGGSYVSSVERALKTYV"
     gene            complement(14837..15313)
                     /locus_tag="VFQ61_05705"
     CDS             complement(14837..15313)
                     /locus_tag="VFQ61_05705"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HET9953974.1"
                     /translation="MLRICFVCLGNICRSPIAEGVMSHLVQAAGLQNEILVDSAGTAG
                     YHEGEPPDARAVAAAARRGIALHGRARQLRQGDYDSFDYLLAMDRSNLEHLLSKAPRS
                     ARSKIRLLRSYEAGADTDAPVRDPYYGSASDFDEVVDVCMAACSGLLASLRESYRL"
     gene            15629..16450
                     /locus_tag="VFQ61_05710"
     CDS             15629..16450
                     /locus_tag="VFQ61_05710"
                     /inference="COORDINATES: protein motif:HMM:NF006133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9953975.1"
                     /translation="MSTLKGKTLLITGATRGIGRAIALAAARDGANIAVLGKTDQPHP
                     KLPGTVHDVVKDVHAAGGRAIPCVADVRFEAQVQKAVDDTLTAFGGIDILVNNASAIQ
                     LTGTADTPMKRFDLMHEVNGRATYMCSKLCLQALRAANNPHILMLSPPLSLRADFFAP
                     HLAYSLSKFAMSFCVLGLAAELAEHGIAVNALWPRTVIATAAVENLLGGTKAMERARK
                     PEIVADAARWILTQPSRTFTGRFCLDEDVLREAGQSDFSRYAVTPDAELLPDLFI"
     gene            complement(16539..17009)
                     /gene="greB"
                     /locus_tag="VFQ61_05715"
     CDS             complement(16539..17009)
                     /gene="greB"
                     /locus_tag="VFQ61_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HET9953976.1"
                     /translation="MPHYLTPEGAKKYAAELNQLLTVERPRVVQEVSEAAAQGDRSEN
                     AEYTYGKKRLREIDRRVRFLTKRLDAAVVVNSSEPRKVDEVRFGASVEVEDENGKKSR
                     YQVVGPDEADPSRGRISFESPLGRSLMKRKVGDVIVVRRPAGEIEVEILSIEYE"
     gene            17142..>18535
                     /locus_tag="VFQ61_05720"
     CDS             17142..>18535
                     /locus_tag="VFQ61_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953977.1"
                     /translation="MRKPPSPRIKLLAAAVLGLFATIALVVGVVVQFVLPRYARSLVI
                     SEARERGIELTPGDISLGMGWVQVSDATFKLVGVRGVTGQAGIVDVELAGRTPKKFTT
                     NRVKLAVTAEPEVLRHELEGWIKRYQKKFEEPLFVKPLDLEYRRSPGESAEIRANGVE
                     IASSNERLSVRVPHLAWSHFSPFPAALDSHGSQLRIRLALANSSLESPLVNLDLDAAD
                     VRLTLAEIRVEQLARWLGLKLDFPGVSVAGNVQFPRPKDLFAFLHTAGSLQSTLKGYV
                     PPHPVELDGFVFGDTTQISTQFALEPENARATLDNFQIVAGRFKLTGKGDVVWEVDHA
                     RMRLALAGELPCASLAGAVAESRLGQALGHLTGKAARQVLGGVVGVRVAVDADSREPN
                     QPKVVRRIVAGCGLRALTLKELMALGELLPEALDPRVAQDFQKLLEKPLPIIPGLTPG
                     ALDPRKPTTLSLPG"
BASE COUNT         3660 a         5696 c         5771 g         3408 t
ORIGIN      
        1 cggtacctag cggcgatgag cttttcgttc actacgtttt ccttactccg cgaacgctga
       61 acgatcgagt tgaggctccc accgaacagg cgcccggagc cgcccacgac gcgggtccag
      121 ccgaaagtcg cggccacaag ccccgagccg cccgaccctt cccctttccg cacgccatcc
      181 cgggtaacgc tctcgcgctt ttggcgcgaa ggtccacggg gtgtttggcg agcgaggcgg
      241 gagcctcctc cagaggttcc ttcgcagaag ttaagtgaac attatccgat ggttgacccc
      301 ccatgtcacc gttgctaacg caccgccggc gcgacccggg atagtttact gcgcgggcga
      361 cctgcgtggg cgacccggga tagttcacgg ggaagaggaa ggaaggtttg tgcggggacc
      421 gcgtatggct ccgtcaggat tcctggagtc ggggctccgc cggagacgat ggtgtctaac
      481 cgggttggaa gcagcggcgg taacgcaccg gcctttgcag cgaacggact aaacagaatg
      541 ctcaaactat cttcggcctg ggtactgggt tgggttgcga tggggctcgt gggtgttgcc
      601 tgcacggtgg tgaccgacgg tgacgacgac cacacgggtg gaagttccag cagcggcggg
      661 cgcggcagcg gcgggcgcag cagcggtgga acgacctcct cggcagaagg aggcatggcc
      721 gaagctgggg aaacgagcac cacgggcggt cgagcctcga ccggaggcag cagcagcgga
      781 ggccggacct cgactggagg tcgaagtagc ggaggcacca cctcgagtgg agaagccggc
      841 gctggcggcg gtaccgacac tccgcccgtc gaactcttcg attgtgactt gcgcagcacg
      901 accggtgccc agttgatcga tgaggacgtc tcgaccaaca cgacttggag cggcaccatc
      961 aatattcgtc gaaacttgcg ggtcgtcgag ggtgccaccc tgacgattga gcccggcacg
     1021 cacatcgtca tgggccccga ttctatgatc gagttcggat ggaacaccaa cgccgccacc
     1081 gtcaaggcag tcggaacggc ggagaagcca atccgcttct gcggcgagca aggagacccc
     1141 ggcttctgga gtggcattag cgtcggctcg aacgtgacct cgaatagcca gctcagccac
     1201 gtgctgattt cggatgcggg cgcggatgag gccgcgctga cgttggcgtc caacatcgag
     1261 atcaccgacg tgcaaatccg caactccgaa aaagatggcg tgcacgcggt ggacttcagt
     1321 gagggcagcg cgcagctcag cgtcgagggc gcgcagggaa ccgcggtggt ggcgactcac
     1381 gccgacgcca tcgatcggtt tcccctcggt ggtaccttca gcaacaacca gaacaacttg
     1441 gttgcactag atttcggctc gatcgaaacg gacgtcacgt ttgccgacgt tggcattccg
     1501 tatcagcacg aaggcacgtt gcaaattagg aaggcaaagg tcgtcatcga tcccggcgta
     1561 acgattctga tgggtgccga cgcattcctc gatgtgggtt ggaacactaa cgcggggacg
     1621 ctcacggcgg tcggtacccc cgaggctccc atcgtgttcc gcggggtcga agaacagtct
     1681 ggattctggg caggcatctt gatcgagacc aacgtaacga ccaattcctc gctctccaat
     1741 ctcgagattt ggcacgccgg tggcaacgac cagtacgcgc tcgaagtcag gtcccccatc
     1801 aacatcgatg atgtacgcct gagtgacaac gatcagggcg cgttcattgg ggcggacggg
     1861 gtctccgacg attcgaaaaa tctctcgatc actcggacga agaaggtcgc gctcacggtt
     1921 gaaccgaacg gcttgattgc gctaccgcaa ggtggtgact tcaccggcaa tgacacggat
     1981 gaagtccaaa tccaaggagg caatttcgag gtcagcggca ccataccgaa cctcggagtt
     2041 ccctatcggg tgctgggcga tatctcgact cggaaggcca gcatgaccct aaccgccggg
     2101 accaagttca tcatgtctgc cgatagtggc atcgaatggg gatggaacgg caacgaggcg
     2161 accatcatcg cggtgggtac ggcggctaag ccgatccagt tcgtaggtgc cgacccggtc
     2221 tccggcttct ggaagggcct tacggtgggc acgaacgcgc tgagctcatc caagttcgac
     2281 tatgtcgagg tcagccacgg gagcaccgcc tgcttgagcc tgaacagctc cgttccggtt
     2341 actcactcca gcttctcgca atgcgagtgg ggtatcttgc acaagacggg tgatgcgcgt
     2401 gactacgccg cgaccaatac cttcacccag acatcgaaag gtaccgtggg caccctgtaa
     2461 acctcgcgtt gagtagcggg acaccactcg cagaagcgca cgggcgcacc caccgggatc
     2521 gcgcccgtgc tgctatttac ggttcaacga gcggctggta acgtgccggc atgatcgtcc
     2581 accggcgctc gaccgcaatt ggcgcggcgt tgtccctagc attgtgggca acgagctccc
     2641 aggttcgagc acagaccaat ccgagtacag aacggcgccc caccgatccc gcggcagcgc
     2701 gtgcccaact caagcaaggc tacgacctga agcagcaagg ccagtgcgaa gccgcgatcc
     2761 tacacttcga ggaaagcgcc agactcgatc ctcaacccaa ggcgttcctc aacttggcgg
     2821 attgcgaaga gaagctcggg cgtttggtgg cggcgcagac ccacctggtc gaagcgcggg
     2881 atctagcgag cagtccagcc gccgcgccgc tccgaaatat cgcagaggag aggctacgaa
     2941 cgctcgaagc ccgcttgccg aagctcgcgt tgcgtttggc gagcaatgcg ccggaggcaa
     3001 cgcgagtgtg gcgagacgac gtggagctcg gtgcggtctc tctgaatgtt gctctgccgc
     3061 tcgatccagg ggcgcaccgg gtggtggtac agcatcaggg cgcgcgccga gactacagcg
     3121 tcgttttggc ggaagcagag agtcgggaac tcgaggtcac cccgttcgcc gacacaccac
     3181 ccgcaccggc gagcggacaa gtgacgtcga agcgtagttc ggagagtgta ccgcaaaagc
     3241 gtctcggtgc ggatacgagc tcgggattac ccattttacc gatcgcgctg acgggcgtag
     3301 cggtcgccgg cgtggtggtt ggcgcggtgt cattggtcag cttgcaagac aagaacgatg
     3361 aaatagatcg agtgtgtcca aacacgaatt gtcctgatca agccacttac gccaagtacg
     3421 aggagctcaa tgattctgcc aaaactgcgc gtctcttcat gattggcggt tttgccgcag
     3481 gggccttggc gggcggggcc agcgcctact tttggttccg agccagccaa gcttcgcaaa
     3541 acggcacgac cgcccgcccc cagttcgccg tacagcccat actgatccca ggcagcccag
     3601 gtctcttggt tcgaggaggg ttctaatgca tcgacgttct cagcagcacc gattgactca
     3661 ggcttggcga cgccctggct tgttgatttg ggccgcactc ggcatcagca ccttgcgttg
     3721 tggggtgagc gagcgacttc cgccgggacc cgtcgatgag ccaggtggag agcgtggtga
     3781 ccccgataaa ggaggcgaat atccaggtgg cgccgaaggt tctgaaggcg gggcaaagcc
     3841 tacgcacggc ggcgccactg ccggcggaat ggtgaattcg gaagctggcg ccaagcatga
     3901 ggcgggttcg ggaggcaccc ttgagccgat cgagaccacg gccggcgcgg gcgctggagc
     3961 gcaatcggac gaaggcgggg cggctgggaa cgctgctggc ggcggcacga ccggcaatcc
     4021 tacgagccaa ctgggaggga cctccggcgg gagctccgta aatccagcaa gtggcggagc
     4081 ctctggaacg cagagcacga ctggagacgg tggatcgagc gggggcgcga cggccagcgg
     4141 cggacaagcc agcggcggtc gaacgggaag cggcggaacg ctcaacaccg gtggtaaaaa
     4201 cctcggaggt gacgacgaac cccgtgctgg tcgccctgcc tgcttcgtcg aaggaacccc
     4261 ggtgacactc gccaacggga ctcaacgtgc gatcgaggag attcgggtcg gagacgaggt
     4321 cctttcgtat gatccagagc agcagacgtt tctgcgcggc caagtggtgc agctgttcgt
     4381 gcatccgcat actccggccc tagtcgagat cgaccatcga gttaccacga cccccgagca
     4441 tcccttcttc gcctcgggcc gtttcaaacg agcggacgcc ctgaacctgg atgaggatcg
     4501 gcttttcgag tggattcagc gacccggcac gcaaccgctc tttggggacg tgggcgcgaa
     4561 cccgagccgt gacgcaagca cgattttcct gagcgcagcc cgggagcacg aactcgagag
     4621 cctcgtcact cgtgctgcgg acgtgacgac ctacaatctg gaggttgtcc caagtcacac
     4681 ctattttgcg gcgggcttct tggtgcacaa cttgaaaccg attccagaaa tcccgtgagc
     4741 gcccttcaac gtagtcgctc cctcgcctca gcgctcagct ctgcctctca gctctgcctc
     4801 tcagctctgc tgcaacattc cagcccagtg agctcacgct accttgggct cgttcgattt
     4861 cccggggcat cgctcagcag cacgactcta gacacgccgt atcgcttgcg gctccgtcaa
     4921 gccgcgcacg cagttgtggt agaaccggcg catggctttc tcttggcgac aagattggcg
     4981 acgtggctcg agtgccgcag tactcgtgtt gttgatcggc tgctcgagtg acaagagcac
     5041 cacccccagc acgggcggaa cgccgggaag caacggaggc caaggcggaa gtatcgccgg
     5101 aaccagctcc ggtggcatga gcacggcaac gggcggaacc accgcctcga cgctaggcgg
     5161 gaacacacag ggtggtgcga gcggcggctc gacgccgact acgagcggcg gcacgagcgc
     5221 gacaggcgga acgagcgtaa gtgccggcgc ctccagcggc ggctcgaccc aagccggcgc
     5281 gtcccagggt ggcggcggag cacaagcagg tggcggagcc ggcggaacga gcggagcgag
     5341 cgcgggtggc gccacgtccg cgggcagcgg cggtggaggc acgccggtag agctaaagat
     5401 accgatcgag cgtagcggaa agtacgtact cgagttcggg gacgtaacct tcagcgttga
     5461 tcccatgatc ggagctcgca tcgcgagctt tgcgctcgga ggcaagagca ctattaccgg
     5521 tcaagatacg gacaccaaag gcgaaggcaa ttggggttcc acctattggc caagcccgca
     5581 aagcacgtgg ggctggccgc caattccgga gctcgacaag aatccgtata cgccctcggt
     5641 tactggaaac acggttacga tggtgagcgc tgtcgcggcg ggaaccaaca agctgagcct
     5701 aaccaagaag ttcactcccg tactcgacgc tcaggccgta gacgtcgagt acgtactcaa
     5761 gaacgaggcg agtcaagcag tgagctgggc gccgtgggaa atctcgcgcg tcccctccgg
     5821 aggactggcg ttcttcccga ccggcaccaa agtcgtgccc gtgacgtcca ccgatccgct
     5881 acccaccgcg aagatcgtga atcaggcggg agtgagttgg tatcagagca gcgccgccga
     5941 caaagggaag tacaacgccg atggtgccga gggctggctc gcctacgcca gcggtgactt
     6001 gttgtacatc aagcgcttcg aagacattcc ggcggacaaa gcggcgcccg gcgaagccga
     6061 cgtcgaaatc tatgcgggcg gggcatacgt cgaactcgaa ccgcagggcg cttatcagag
     6121 catgccggca cagggcacgc tcagctggaa agtgcgctgg tacgtgcgca aactcagcga
     6181 tacgagcatc gtcaagctgg gcaacgccga tctcctcgcg ctagtccgcg aggtcatcaa
     6241 gaaataacgt gcacgcaagg cggcattcgt cagccgtacc cgtgagttcg gacgcacgag
     6301 ccagagagtg ctcgtgtggt cgagcgtgga gcggctgaca gtgggcatcg aagaactgga
     6361 acggcagcgt ctgtgggacg cggcacaggt gccttgggaa gtgctcgatt acgagcgctg
     6421 gattgcccaa gtctccgcga ctccgaccga cgactattta ttttccagcg ccaaagctcg
     6481 cttcgaagct gcagattccg atcgactgct ggcgctcgtc gacgtcgaga ttcagggccc
     6541 ggcgtctgca ccgaagctct actccaaatc gctcgcggtc agcctctcgt tgtccggttt
     6601 gtctcgccag aatgcggagc agtgtttatt ggcattgcgg tcgaatagga ccgtggcgga
     6661 gctgcggagt gagttgcctt cgatggatgc gttcctcacg cagacgctcg gcttactcgt
     6721 ttttgcgccc gaagccctga cagagctcga ggctcgagtt cccggaaccg agctcgtgcg
     6781 cttcgtgggc accccctacg agattgtgcg agcatattgg gagaacgcgg cggacgtgcg
     6841 cgtcctcgcg gaggatatct tatctggagt ccgggacggc gcctcatttt tggagtcgct
     6901 gcgtgccctg cacgtggtca cgttgatggg ccgtaggctc gatcgtttct attgccccgc
     6961 cagcaaggtg gcgcgcgcgg gggtgcgtcc ggggatgctg ttcgcagcat ccactgcact
     7021 gcaatccacg gcccgagact tactcttgct ttcggggccg cgcgttggtg caccctacgt
     7081 tggcggtgcc cgctatcacg agctgctcac ctggggactc gtcgcacgcg ctgaccgggc
     7141 caactcgtct gcgaccgcat cgtgcccacc ccagccatcg aggcatgacc aatcaggcca
     7201 tgtcagcgac tggggcagag taattcgggc gcgtgcgccg cacgaggcta gcgtcgaaag
     7261 ctggttcatc cctccaaggc ccatgacctc cagccatttc gacgcgcttt ggtctgccta
     7321 cgcggaggct acgcaggctt cccggcgagc ggacactccg ggcttgtatg atgcgcttgc
     7381 tcgattccac tatcgttttg cgagattgca tccgttccga tgcgccaacc agagcctgag
     7441 catgaaccta gtgaatgcgt tgctccaaag caccggcgga ggcattcccc acctattgtt
     7501 ggaccacgtt gctttccacg tcgacgagcc cgcttatgca tgcgtgttcc aacgggcagt
     7561 gaagcatcac cgattaatca ccggcagtgc gccgccaaac ccgagccaac gttggcagtt
     7621 cttacggcag agaaaacaac agtcgtacgc gctggtggag aagatccaaa aggcaaactc
     7681 gctcgagcac gcaaaacaga tctgcaaagc agatccgacg ggaagcgagg ccgcactgat
     7741 tgacctgaac gcgctggctt gatctggccc taggattggc gatgcccggt cgagcggggc
     7801 tgcaaacagc ggcgtatctc gggcatccgt gggcacgcgg cagcgctctt ttgcgccgcc
     7861 gcgctgcccg atcgggtcac atgcccaaca gatatgctgc gatcagcggg aacacgatcg
     7921 tcgcgtcgga ctcgatcacg taacgcgggg tatcgacgtt cagcttaccc caggtgatct
     7981 tctctccggg aggcgcgccg ctgtacgagc cgaacgaggt cgtcgcgtcc gaaatctgac
     8041 agaagtagcc ccaggtcttg acgctccaac ccaagtcctg gtccatcaac ggaacgacgc
     8101 agatcgggaa atccccagag atgccgcctc caatttggaa gaagcctatc gaatgttgct
     8161 ggtcgacttc cttgtaccac tcgatcagac ggcccatgta ctcggtgccg cccttcatga
     8221 tttggtagcc atactcgccc cgaatcacgc cggccacgaa catgttgccg agggtcgagt
     8281 cttcccagcc cggcacgaag agcggcaaat tcttctccgc cgcggcgagc agccacgagt
     8341 gtttgggatc aatctcgtac tcggactcga gctccttgct gagcagcagc tgataaaaat
     8401 actcgtgggg caggtagcgc tcacccttct tttgcgctcg ctcccaaagc gccagcatct
     8461 tcttctcgat cttgcgcatc gcctcgtgct cggggatgca agtatccgtc acgcggttca
     8521 gccccttgtc caacagcgcc ttctcgtctt cggggcgcag ctcgcggaag ttcgggaccc
     8581 gccggtagct ctcgtgcgcg acgagattga acacatcctc ctcgaggttc gcgccggtca
     8641 gggagatggc gtgcaccttg tcttggcgaa tcagctcggc gatgacgatc ccgagttcgg
     8701 cggtgctcat ggccccgccc atagtcagaa acattttgcc gccctgggcg aggtgcgcct
     8761 cccagcctcg ggccgcatcc acgagcgcgg cggcgttgaa gtggcggaaa tgatgctgaa
     8821 tgaaatcgcg gacggtgccc atgactgctc caaagtgggt acgaggagcg tctctcttcc
     8881 gcgtttcacc cggcgcgtca agtcggcccg ctccaaaccc acctggcgcg aaacgccagc
     8941 aacccctgct cgcaatctcc tgggctgcag cgcgcagaac ggtgcgttgc agaaatttca
     9001 tggtagcctg cgcccgtgaa ccaccatttc gtctatgaac gaagtggcgg gacccacgag
     9061 cggtcctgcg tggcaacccg ggaataccgt ctgccactgc acaccggtgg cggtttgggc
     9121 cccctccgcg ccgcggcctt gcgcggagct ggcaacaacc cgggaacact gcgcttcgga
     9181 accctgcgct ccggtgaatc gcgcaccgcc agcagcaacc cgggaacact aggcttcggc
     9241 gaatcgcgca ccggcccgcg tgctgctcgc gacccccacg tcgtggccaa gctcgacccg
     9301 ggaaggcttg cgactcagct agcagcatcg gtgcggccgc gacgcgcagg caacccggga
     9361 acccgtgcga cgcaggtgga gccgttgttg gatttgcgat cggggaccct taaaccccgt
     9421 gccatcctgg agtgctgcgg catgtcggta tcccgggacg ccaaagcaag cgggacgcgg
     9481 agcttggctg gaagcagcgg agatacgggt tcctttgatt tgcagacggc ttcgacatgc
     9541 cgaccccact gcgcagtttt gcgagatctg ggtagtttcg cagcaggttc ctctgttcct
     9601 gaaactcccc gaaagctcga tgtgaaaaag tactagagca acgcggcgac tcggattcaa
     9661 tccatagaca agggatccgg gtcatgcagc ggccacttcg gccgggcccg ttccgcgaaa
     9721 gcactctctc atgatggacc gatattcgac accacctccg gcaaccctga gcggaatggt
     9781 cgtgtactcg gaggtgcaac cgggcgtgag ctatcgcctg gaacgtcaaa tcggcgaggg
     9841 aggcacgggg atggcctacc tcgcgttacg gcaagcccaa gatggcctgt cgccggtggt
     9901 cctcaaactc gttcatacgg atccgacatc ggagccggcc gcggatctgt tcgtacaaaa
     9961 ggaagccgtc gcgctggggc gcttgaacga gcgcgttccg cctaccccgt tcgtcgtgcg
    10021 cttcatcgac accgggacaa cccgggtgct cgacgacacg ccgatgccct ggatagctat
    10081 cgagtacgtc cacggaggta tcgagggcac cacgctggag gatcgagtcc tttattcaat
    10141 tcaccaaact ggctgcgcct tcgatccgcc tcgagcgttg cacgtggtcc ggtgcttggc
    10201 gcagggcctc ggagcgatcc acgccgtcgg cgtcatccac cgcgacatca ccccgggaaa
    10261 cattctgtgc tgcggcttcg gtgaagcgga aatcttcaag atctcggatt tcggcgtcgc
    10321 tcgaccgcaa gggttgacag ccacttttgg agggctcgga gtcggcacgg ctggctatgc
    10381 ggcgcccgag caaagcttgc cagatcacac cgtagtcggg ccgcacaccg acgtgtttgg
    10441 gctcgcgtgc gtcgtctact acgtgctcgc tggcgagcat ctattcgatg ctcaatcgcc
    10501 agtgcaggcg tactccatga tcaaggataa ggctcgtcga agcatctgtg agagtcgtac
    10561 cttgagcccg gagtttaccg agcagtcgga cgtgtgccgc gcgatcgacg gggcaatcgc
    10621 acgggcaacg tcatttgctg cagatcagcg gccggcatcc gtgcaagagt ttgccgcgaa
    10681 tatcatcccg tggctcggag cgccgagctc tgcaccgcgt cctagtcggc ggttggtgca
    10741 tagtgtgctg cagctcacgc cgcgagggga tctctctggt tggagttgga ccgtacgcca
    10801 ccctcccgga gacaacgtgg tgattcagag tgcggcctgg gacgcggacg gccgatgctt
    10861 tgcttttact ccacgaggcc cgttgttttg gaacggacaa tcgtggctac tcgcgccgga
    10921 agcttcacga agtctcccgg aaggaatggg cttcgccaaa cgttatgaag cgggtggttg
    10981 gctggttggt ggcgccgggg gagtgctcgc gatttacgga acagaaggcg tgcgcgaggt
    11041 tattcatgcc cccgacacga agacgcgctt tttgcacgca agtggccgtt tcgacgacct
    11101 gctcgtagcg gtcgcgcaac gcccgtccga aacgcctgca ttgtgggcga tgaccgctcg
    11161 ccgatggatg aagccgctcc acctcgacgg tgtcgcgtac gtctcggggc tacatcgctt
    11221 ggacgatacg cgctgggtgg tatgcgggcg aatgaccgag ggggtcggtt tcgtggcgat
    11281 ctacgcgccg ttggattggg aggtcacttt cttgaccacg cctcatacgc gagctttcgt
    11341 ggcgggagct agtgaacccg agcgaaatct agcgctgttg gtgggggcgt cgggcgtgac
    11401 ggtgcgtcta gatggagaaa cggcgatgag cgccgtagcc gagggcgaac ctgatctcac
    11461 cgcgagcgcc atggacgtgc tcgcccggga atgggtcgcg agcgtcagca cgttgtggat
    11521 gcgtgatccg gaacaccagg actggcggac ggtttggacg agccctgagt ttcggacgcc
    11581 tttcattagt ctcatggccg atgccgggtt agtggtggcg atgacagtgg atggcggcat
    11641 cgtggaggga cgggcggcat ggcgaggccg tcgcccgcgg cgctaaccaa cactcaagcc
    11701 aagtcctacc ccaaacacgg cgcccgcgaa tatacccggg ataccttcgc ggcagacaaa
    11761 cccgggatac gtttgcgaac aactgggacg ggctccggac taagaagcca ggggactcga
    11821 gctccggaaa agtggcacac aagacctgca agcggagccg cagggacgac ccgggggctc
    11881 tccagaaaca gaagcgcgta ctctcgtgag tgcaacccac cactcagcct ggagcattcg
    11941 gttgcctgag tcgtgggcag cccgctatga agctatggtg ggaattagga cgctcgagga
    12001 gatcgacgcg tggtccgtgt aatcgaaccg ttgccccgtt gagacgcaaa tctgttcttc
    12061 tgcgaaggcc ccgaaagaaa cttacacgag agcgggcggg cgctacgcaa tggcatatag
    12121 cgtggcgacg tcttcggctc gctctccggt gcccctctgc cctagcccat gcctgcgagt
    12181 aaaacacaga ccctagtgat tcggaaacga ctaactcggt acggtcggaa cgcacctgac
    12241 tccggcgaag atgcgcaaag cataggtgcc ggctggaaag attacgtctc ttgcccctct
    12301 acgccaaagg tcccgggcgg ctctgtatgc ggcgacaaag tgtctgaact gcgcttgcat
    12361 cgactttctg atcaattgat tcttcgcgac aattcgagga acgaagggcc tcgacgtggg
    12421 cgccgtgtcc ggcatagact cgacggattg tctgagcacg aactcccgcc ctctaaactt
    12481 gcggccctca ctctcgagct cggccctcgc ttttcgtacg taacccgaaa ctagatcgtt
    12541 gagctgtctg gccacttcgg cgtcgctcag ctggagcagc actggcggac gggagaagca
    12601 gagttcaaga ctttcgggca tgtcatcttc gatatcgtcg aggtcatccg gaatagagac
    12661 cgtgcgacgt tcgtcgataa aacgcgtaat gactccaggc cattcctccg gtgagtggac
    12721 caggccagcg agcgtcggat tggagagcgt ataggccatc ttttcgagga tgtcttggtc
    12781 cgaaccgagc atcaggacac tcggcttgtc cgaggaccac aggttctccc gtcgccctag
    12841 agccgcattc tgcgccttgg cgaaaagccg atggaacctt tccaggaact ccggcaatcg
    12901 agcgaatgga tcgctgagca caccatgcca atgattggac atgaagcata ctgcgtgtaa
    12961 cagcaccccc gtcttgtagg ccgccaacgc cagacagtag ctgattagga ggttggttgc
    13021 tctcgagggc cgcaaaagga acatgcgctg ggtgcagcgc ctcgtcacga agtacgttct
    13081 gccgggtaga atttgcctgg gtagggtcac ctcctctcac tcggcccggt tcgggcgaga
    13141 gttgcttagt ttcttccgct atcctttccg cgtaaggcgg aagaggcgtt tgccgtcgcg
    13201 ttcggtgggt cgctccacgt tcagcattgc cgtccgaatc tccctgatgc ggccggggcc
    13261 accgagatcc cggatcatca gcttgtcatc gtttgcggtg agaacctcgc aaagtccgaa
    13321 cgcgaagtgt tgcaccaggt caccgcgttc cggctctgcg ggttccggtt cctcttcggg
    13381 aacggcctgc gctgcggcta ctgcgttgac ttgggcagcc caacccgccg cgacctggac
    13441 tcccccctca gccgctttgg aactcatccc gggaacggag gcgttggaac tcatcccggg
    13501 aacggaggcg ttcgtggaag ctatcccggg tacgggagca ttggtaggga tgggtgccgc
    13561 ggcgggcgcc gatgtccccc cggtcgactg tgccaaagtc acctccccat cgccagataa
    13621 gcagagcgcg tgcacaccgg ccgaacgcgc tgaaaccagg atgccggata ccaagtcgac
    13681 cgcgcccgaa gaatcgaccc gggatagtgt tgcaccgtaa gggccaccca gtgggccggc
    13741 gaactggctc agcgcatatc gccctcggaa gagtcgtcgc gtgtccgccc cgtccgtggg
    13801 caggcgaact tctgcttgct cgacgtaacc agtcgccaaa atccaagatc ccttggaacc
    13861 gaacaaatcg gaaagcgact caatcaaatc tcggccatcg actatcgtgc gaaaagcggg
    13921 gggtaaccta gccacgggcc gtatatatcc cggatcgagt cgagtcacac gtaggtcttg
    13981 agcgccctct cgaccgacga cacatagctt ccgccgaata ggttcacgtg aaccagtagg
    14041 gggtacagct ggtacaaccc gacacgttcc tgggccccag tttcgagcgg caacgcctcc
    14101 gcgtaagcgt cgaacacacg ttgggaagtg cccccgaaca gccgcaacat cgccaagtcg
    14161 acctcgcgat gtccgccata ggcggcgggg tcgatgaggc aaggttcgcc gcgatcgtcc
    14221 acgtggacgt ttccgctcca caaatcgccg tgcaaacgcg agctcgactt cacctcgccg
    14281 agcagccggg ggattcggcg ttccagttcc tgaaagccgc gtcgcatgag caggcttacc
    14341 cgcccaagcg atactgctcg acgaagctgg ggctcgagcc gacgtgacca aaagaactcg
    14401 gcccaggttg cggtggggtg gttctcctgc ggcaaggatc caatgaaatt gtcgtgatct
    14461 agtccgaact caggggcggc ggccgcatga agcttggcca gtccccggcc gagcacgtcg
    14521 tcaaagtcac gggagggtct cccgggttcg agccattcga gcatcaagaa gctcgaggtg
    14581 tcgcccgcgc gagaacacgc gagcacctcc ggaacgcgaa gcgccccagc ctcccggagc
    14641 cacttcaggc ccaaagcctc gacttcgaac atctctggcc gcgccgtggg attcgccttc
    14701 aagaagcaga gactcccatc atcgagctca caccggtagg cttcattgat gtcgccgccc
    14761 gccacgcctc gccaattcgc cacctcgaag ccacattccg agaggtgacg acgaagactg
    14821 tcaggccagc ccttcgtcat agccggtagc tttcacgtag actcgccagc aggccactgc
    14881 aggcggccat acaaacgtcg actacttcgt cgaagtcgct cgctgagccg taatacggat
    14941 ctcgcacggg cgcgtcggtg tctgctccgg cctcgtagga cctcaacagg cggattttgc
    15001 tccgcgccga tctgggcgct ttagatagca ggtgttccaa attcgagcgg tccatcgcga
    15061 gcaggtagtc aaaactatcg tagtcgccct gacgcagttg acgagcgcgg ccatgaagag
    15121 caatcccccg ccgcgcagca gcagccacgg ctctggcgtc cggcggctcg ccctcgtggt
    15181 acccggccgt gccagcgctg tcgaccagaa tctcgttctg gaggcccgcg gcctgcacga
    15241 gatgagacat gacgccctcg gcgatcggag aacggcaaat attcccgaga catacgaagc
    15301 aaatccgcaa catgcccctt actgtagaac atctcccggg agagtacgcg aggcgggagg
    15361 aagtgcagcg agcgggtcaa aaccgagccg agcggatcat ccaatagatt gccacccccg
    15421 ctccaaccag actccccagg agcaccgtca atagccagcc cgtccgccga acaacgctgc
    15481 gctctgggtg gggccgactg ctgtcgaggg accgggacgt acgactcatc gcccgaacct
    15541 accctggata cccgaaggcg gcgagccgcg ccgctcgacc tagagcaagg gcccgaactc
    15601 ctgagcttcc ctggtatacc tgctcgtcat gagcactttg aagggcaaaa ctctcctcat
    15661 tacgggtgct acgcgaggaa tcggccgcgc tatcgcgctc gccgcggccc gagacggcgc
    15721 caacatcgcg gttttgggaa agacagacca gccccatccc aaactgcccg gaacggttca
    15781 cgatgtcgtc aaggacgtcc acgccgcggg cggccgagcc atcccctgtg tcgcagatgt
    15841 gcggttcgag gcccaagtgc aaaaagcagt ggacgacaca ctcactgcat tcggtggaat
    15901 agacatactg gtgaacaacg ccagtgccat ccagctgact ggcaccgccg atactccaat
    15961 gaagcgattc gatctcatgc acgaggtcaa tgggcgcgcc acgtacatgt gctcgaagct
    16021 atgcttgcag gcgctgcgcg cggccaacaa cccccatata ttgatgcttt ccccgccctt
    16081 gagcctgcgc gcggacttct ttgcccccca tcttgcatac agcctgtcca agttcgcgat
    16141 gagtttctgt gtgctcggcc tcgccgcaga gctcgcggaa cacggcatcg cggttaacgc
    16201 gctgtggccg cgcaccgtga ttgctacggc cgccgtcgag aacttgctgg ggggcacgaa
    16261 ggccatggaa cgagcaagga agccggaaat cgtggcggac gccgctcggt ggattctcac
    16321 ccagccgagt cgcaccttca cgggaaggtt ctgtctcgac gaggacgtgc tgcgggaagc
    16381 agggcagtca gacttctctc ggtacgcggt cacgcccgat gccgagttgc tccctgacct
    16441 gttcatctag gaggggttcc gtttcaagga tttaagccct tcgttctcgc ccaacggggc
    16501 aatgcctcac tctggccttt tcgccggaag cgtcgggctc attcgtattc gatgctcaag
    16561 atctcgactt caatctcccc cgctggccgc cgcaccacaa tcacgtcacc gacttttcgc
    16621 ttcatcaaac ttcgtcccaa aggagactcg aagctgatgc ggccgcgtga agggtcggct
    16681 tcgtcgggac cgactacttg atagcgactc ttttttccgt tctcgtcttc gacctcgacg
    16741 ctcgcgccga aacgcacctc gtccacctta cgtggttcac tcgagttcac gacgaccgcg
    16801 gcgtcgagtc gcttggtcaa aaagcgaacc cgcctatcga tttcccgcaa gcgcttcttg
    16861 ccgtacgtgt actcggcatt ctccgagcgg tcgccttgag cggccgcctc cgatacctcc
    16921 tgcacgactc gcgggcgctc cacggtcagc aattggttga gttcggcggc gtattttttg
    16981 gcgccttcgg gcgtgaggta gtgcggcaac tcggggctct ccttgcgcgg acgttaacat
    17041 gcacttacag cccacgccgc cctgggccta tgctgcggac gtcttttcgt gcggccgaag
    17101 ttctgacaaa catcagctag aaaacggccc cggagcccac catgagaaag cccccttccc
    17161 cgcgcatcaa acttctcgca gccgcagttc tggggttgtt cgcgaccata gccctggtcg
    17221 tcggggtggt cgtgcaattc gttttgccac gctacgcacg aagtttggtg atatccgaag
    17281 ctcgcgagcg cggtatcgag ctcaccccgg gagacatttc cttggggatg ggctgggttc
    17341 aagtgtcgga cgcaacgttc aaattggttg gtgtccgggg cgtgaccggc caggccggta
    17401 tcgtcgatgt cgaactcgcc ggtagaacgc ccaagaagtt cacgaccaac agggtcaagc
    17461 tagccgttac ggccgagccc gaagtcctcc gtcatgaact cgaggggtgg atcaaacgct
    17521 accagaagaa gttcgaagaa cccctgttcg tgaagcctct ggacttggaa tatcggcgct
    17581 ccccgggaga gagcgcagag attcgggcga atggcgtgga gatcgcaagc tccaatgaac
    17641 gactgagtgt acgggtgccc cacctggcgt ggagtcactt ctcaccgttc ccggccgcgc
    17701 tcgacagtca cggctcccaa ctgagaatcc gcctcgcttt ggcgaactct tcgctggaaa
    17761 gcccgctggt aaatctcgac ctagatgccg ctgatgttcg cctcaccctg gcggagattc
    17821 gggtcgaaca gttggcacgt tggctgggtc tgaagctcga tttcccgggc gtctccgtgg
    17881 caggcaacgt tcaattcccc cggcccaaag atctattcgc gttcctccat accgcgggct
    17941 cgcttcaatc gacactcaag ggctatgtcc caccgcaccc cgtcgaactc gatggcttcg
    18001 tgttcgggga cactacgcag atttctaccc agttcgcgct ggagccagag aatgcccgtg
    18061 cgaccctcga taatttccag atcgtcgctg ggcgcttcaa attgacaggc aaaggtgacg
    18121 tggtttggga ggtggatcac gcgcggatgc ggttggcgct cgcgggcgaa ctgccttgcg
    18181 cttcgttggc tggggcggtc gccgaatcgc gtctcggaca agcgctaggt cacctcaccg
    18241 gcaaggccgc gcggcaagtt ctgggaggtg tcgttggcgt acgggtggct gtcgatgccg
    18301 attccaggga acccaatcaa cccaaggtcg tgcgacgaat cgtcgcgggt tgtggcctac
    18361 gggcgctgac actgaaggag ctgatggcgc tcggagaact cttacccgag gcgttggacc
    18421 ctcgggtcgc gcaggacttt cagaagcttc tagagaagcc gttgccgatc attcccggcc
    18481 tcacgccggg agcgctcgac ccgcgcaaac cgaccacttt gagcttaccc gggat
//
LOCUS       DASTKU010000159         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_921168, whole genome shotgun sequence.
ACCESSION   DASTKU010000159 DASTKU010000000
VERSION     DASTKU010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921168"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..645)
                     /locus_tag="VFQ61_05725"
     CDS             complement(19..645)
                     /locus_tag="VFQ61_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HET9953978.1"
                     /translation="MFGRLLPKETSFFDFFEAHAELTVAGAKEFMTLVSHGANIDARA
                     KRIKEIEHETDVITHRCVEALHKTLITPIDPDQIHRLITKMDDVMDYIEAASERIALY
                     KLRDMTQEVQDLADVLERSAKEMLEACKLLRNLKDPQTILKRCVDINRLENEADAILR
                     RAVARLFDEEQNPITVIKWKEIYEHLESATDRCEDVANIIEGVVLEHS"
     gene            946..2118
                     /locus_tag="VFQ61_05730"
     CDS             946..2118
                     /locus_tag="VFQ61_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9953979.1"
                     /translation="MILADFLVALGSGAVVWLLLARPALRQSRQLRRASQELSESYAA
                     TVRDLKRERDLLGGILETMQEGLLLLNEQDEIVLLNPSLRSTLLLPADVVGKKLSDAL
                     QREELNELVRQARGTHKPAASELSVSGLKPRRLAVRANPMPAPSSAVLLVFFDVTEVR
                     RLESMRRDFVANVSHELRTPVTAICSATETLLGGALKDLDAAPMFVQIIERNSERLRA
                     LVEDLLDLSRIESKEYKLERTELDISTIVERALSLIREKAQKGRVRIDVELAPDLPVA
                     IADARALEQVLLNLVENAIKYCPPGSRVVVRARVDRAGYVRIAIEDTGPGIPERDLPR
                     LFERFYRVDAGRSREMGGTGLGLSIVKHLVEAMGGEVGVQSRVGFGSTFWFTLPTV"
     gene            2331..>2731
                     /locus_tag="VFQ61_05735"
     CDS             2331..>2731
                     /locus_tag="VFQ61_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953980.1"
                     /translation="MPLLESEPRPELNSNLEAANASPGPEPSGPYETGTRISTADPHL
                     TSDGERTQGIGADSNPSEAASSQKQRSAVEPKPPESHASESRGAVQSLEFSYDNFDIV
                     PSWEMWGPYSAESASRINVVREAEPDAESVGA"
BASE COUNT          490 a          831 c          872 g          538 t
ORIGIN      
        1 gtccatggtg ggtcggggct acgagtgctc gaggaccacg ccctcgatga tgttggcgac
       61 gtcctcgcag cggtccgtcg cgctctccag gtgctcgtaa atctctttcc acttgatgac
      121 ggtgatcggg ttctgttcct cgtcgaacag ccgagccacg gcccgtctca ggattgcgtc
      181 ggcctcgttc tcgagccgat tgatgtcgac acagcgtttg aggatcgtct gtggatcctt
      241 caagttgcgc agcagcttgc aggcttccag catctctttc gccgagcgct cgagcacatc
      301 cgcgagatcc tgcacttcct gcgtcatgtc tcgcagtttg tagagggcga tgcgctccga
      361 ggccgcctcg atgtaatcca tcacgtcatc catcttggtg atcaggcgat ggatctgatc
      421 gggatcaatc ggagtaatca gcgtcttgtg cagcgcctcc acgcaacggt gcgtgatgac
      481 atccgtctcg tgctcgatct ccttgatgcg cttggcgcga gcgtcgatgt tcgcgccgtg
      541 actcaccagg gtcatgaatt ctttggcacc ggcgacggtc agctccgcgt gcgcttcgaa
      601 gaagtcgaaa aacgaggttt ctttgggcag caggcgacca aacatggccc cgcctctacc
      661 acggcggttg cgaccttgtc attactgtgt cacgcaattg tcacatgcgg atgtggattg
      721 atcggggcgg tcgtcattcc atcccggtaa ccgcgtccaa tccctgctcg cccgcgcgaa
      781 cggccgctgt gggcacaaga aaacagttgt aaacgctgcc gcctgagccc aggcaggcgg
      841 gcttcgattt cgcgcccctc ggtcgtgtgc cgtagcaagg gcacccgcgc tttaattgct
      901 tgtgagtgag cgagcccgtc attagcgtgc gacgcactgg acggcatgat cctcgcagac
      961 ttccttgtgg cgctcggctc gggcgcggtg gtttggctgc tgttggcgcg gcccgcgttg
     1021 cgccaaagcc ggcagcttcg gcgtgccagt caagagctgt ccgaaagtta cgccgcgacc
     1081 gtgcgtgatt tgaagcgaga gcgcgacctg ctcgggggca tcctcgagac gatgcaagaa
     1141 ggcctgctgc tcctcaacga gcaggacgag atcgtactgc tgaatccctc gctccgctcg
     1201 actctgctgc tgccagcaga cgtagttggg aagaagctga gtgacgcgct tcagcgcgag
     1261 gagttgaacg agctcgttcg gcaggcacgc ggcacgcaca aaccagccgc ctcggagctc
     1321 tccgtgtccg ggctcaagcc gcgccgattg gcggtgagag cgaacccgat gccggctccg
     1381 tccagcgccg ttctgctggt gttcttcgac gtgaccgagg tgcggcgctt ggagtcgatg
     1441 cggcgcgact tcgtggccaa tgtctcgcac gagttgcgca cgccggtgac cgccatttgc
     1501 tcggcaacgg agacgctgct cgggggagcg ctgaaggact tggacgcggc cccgatgttc
     1561 gtccagatca tcgagcgtaa cagcgagcgt ctgcgcgcac tcgtcgaaga cttgctcgac
     1621 ttgtcgcgca tcgagtccaa agagtacaaa ctggaacgta cggagctcga catctcgacc
     1681 atcgtcgagc gcgctctttc tttgattcgc gagaaggcac agaaggggcg tgtgcgcatc
     1741 gacgtcgaac tcgcgcccga tttgcctgtc gccatcgctg acgcgcgcgc gctcgagcaa
     1801 gtgctcttga acctcgtgga gaacgcgatc aagtattgcc ctccgggctc gcgcgtggtg
     1861 gttcgagcgc gagtagaccg agccggctac gtgcgcatcg cgatcgagga tacgggtccg
     1921 ggcattcccg agcgtgattt gccccgtctg ttcgaacgct tctatcgcgt ggacgcgggt
     1981 cgatcgcgcg agatgggcgg gactggtttg gggctatcca tcgtcaaaca cctggtcgaa
     2041 gcgatgggtg gtgaggtcgg ggtccaaagc cgggtcggtt tcggatccac gttctggttc
     2101 acgctgccca ccgtctgagc ccgcgcggcc cgaagcccat gcccatcccc atgcgatggg
     2161 atggctgata agtcgcaggg gccgcgccat tcgactcgtg ccgagtgaga attcttcaat
     2221 cagattggcg cgggtcggcc gaatgcgtcg aggaacccag ccgtagagcc ttgcgcctat
     2281 ggcaagctgc gagaaaatcc gaagaccact ccgaacccca cgagacccgc atgcctttgc
     2341 tcgagtccga gccgcgccct gagctgaatt ccaatttgga agccgcgaac gcgagccctg
     2401 ggcccgagcc gagcggaccc tacgaaaccg gaactcggat ttcgacggcg gacccgcatc
     2461 taacgagtga cggggagcgt actcaaggaa tcggcgccga ttcgaaccct tcggaggcgg
     2521 cgagctccca gaagcaacga agtgccgtgg agccgaagcc ccccgaatca catgcaagcg
     2581 agtcacgcgg agcggttcag tcgctcgagt tctcgtacga caatttcgac atcgtgccga
     2641 gctgggaaat gtgggggccg tactcggcgg agtcggcgag tcgcatcaac gtcgtgcgag
     2701 aagcggaacc agacgctgag tccgtcggcg c
//
LOCUS       DASTKU010000160        12845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1814200, whole genome shotgun sequence.
ACCESSION   DASTKU010000160 DASTKU010000000
VERSION     DASTKU010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12845
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814200"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFQ61_05740"
     CDS             complement(<1..209)
                     /locus_tag="VFQ61_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953981.1"
                     /translation="MDPGERWYFDELVPACELGELRCVSSRLERCVAVGPRRTYQLEQ
                     ECADDGLTCAPELGRCVSCLPGTAR"
     gene            complement(285..2378)
                     /locus_tag="VFQ61_05745"
     CDS             complement(285..2378)
                     /locus_tag="VFQ61_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HET9953982.1"
                     /translation="MRFRLQTALVILVGASLLGGCRASGKASLPRIEDDGSDLPPTLD
                     AGTTRPDEEPEALPHALLGIEPPHGPFSGGTLVLLRGNGFAGNTRVWFGENEIPSEQV
                     LSVDSHRLQVTAPAGEAGPVDVAVQNGDDVSTRVVLRGGFSYDAFYVEPSTGPTAGGT
                     LVTLHGQGTSWDSDTQIQIDDQPCELVELLSPSELTCRAPAGSPGAKPVSVTSGEERI
                     DVLDGFAYTNSDNGFRGGFTGSPLRDELTVLVFDSIQGEALPGASVIVGVDDPEVERT
                     DRDGVAVVQQRELGPKQTITVVRRCFQPVTFVDVPVDRLTVYLDPVLSPACFDPKGDL
                     EAPGGTPSDTAVVSGGLVWPLDGEFRRHDWGNVPAPKTDSEMLAAYVFALASRPTDAF
                     SLPSAVGTITPDTNEISGASFSLFTRPGTYTLYALAGVENRRRQPFTFTPYAMGLVRG
                     VPALSSGQTADVYIEVNVGLDHALRIDVAGPKPTRRGPDRVEGNLALQVGTEGYVLLP
                     GGQKSALLSAGSTLEFVGIPPLSKGLAAANYVVSLRAVTGDAGTLPESVLARVVTRDT
                     SEVLDPGAFLEIPVLTEPEPNGEWSGRELAFTHVAGGPSADLALVDLTSNSDLYHWQI
                     VAPGNVRRVRLPDLGAISTDIAWPSGQQLVTLTLARLPNFDYASLRYRDLTDRAWAAY
                     ARDAFFLDHASSSDY"
     gene            complement(2548..5016)
                     /locus_tag="VFQ61_05750"
     CDS             complement(2548..5016)
                     /locus_tag="VFQ61_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9953983.1"
                     /translation="MPDPPWKRSVGVGAVVDAWQDAGSVKRCLAAERALPAREAVFEP
                     LPDELSEAARRALAGRNIRQLYSHQVQAIRAALEPKHVVVATPTASGKSLCFHIPVLE
                     RLARDPKATALYLYPTKALSRDQEHGLLGLLADAGLPTQAMVYDGDTPGDARRAARER
                     CRVVLTNPDMLHSGILPSHARWASLFEGLTYVVLDELHTYRGVFGSHMAHVLARLRRV
                     AAFHGKQPTFLAATATIGNPREHAARLFDCKPEEIALVDRSGAPAAAREVYVYNPPIV
                     NADLGIRGSTLKHSVSLAADLVEARVPTLVFGPSRNSVEVMLKYLRARVGNVAGPEAI
                     MGYRGGYLPETRRRVEAGLRNGEILCVVATNALELGIDIGDLDAVVCAGYPGSIAATW
                     QRFGRAGRRGDRSVALLVCSSSGLDQYLASDPEYLFRAGAEEARIDPSNLEISLQHLK
                     CAVFEAPYEVSARGSKPADPAPAQGERHLGLDLESTRDALDYLASLGVVHASDGRYHF
                     AAEAFPASNVSLRNIGWDNFVIIDVGTGKSLAELDYRAAHTMLHEQAIYQHDAEQYQV
                     ERLDFDNHKAFVRKVEPDYFTTALTYRTVSVLETAQTRPIGRALVGHGDVKVLSRVTG
                     YKKIKFFTHENAGYGDVHLPEIQMHTTSFWLTVPERIIEELGEARSSVIDALRALGSA
                     LETVSTLFLMCEPRDLGQTLGDGSTLGQAGLGPADFREAGSELAASGPGQVAAVGHPP
                     ERDPFARHTGQFDPTLFLFDAHAGGVGFAPRIYEQIEVLLERVATLVSRCACGFGCPA
                     CIGPSDSDSVRKKLALQLLARLQV"
     gene            5113..5904
                     /gene="lspA"
                     /locus_tag="VFQ61_05755"
     CDS             5113..5904
                     /gene="lspA"
                     /locus_tag="VFQ61_05755"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET9953984.1"
                     /translation="MSESASPDAQDLSAAPGAPTPAAPEPLTNAASVSAASKSGAEPH
                     AGVGLAEGPKRSRPRFVFFGIVSAVTLLLDIASKAWAEVTLTSRPAHQPSITVIADHL
                     SFTLAYNRGGAWGLLQDAHESIRLLFFLTVNIVALLFITWLYSKLVRGQRALMWGLPL
                     VMGGALGNFSDRITRSSVIDFIDYRAQWVLAMNRLIGKVSPNWTITDHWPTFNVADIA
                     ICVGVGLIGLDMFTSKHGPVQQDPGSPLQPGANRESSSSETAPSA"
     gene            5912..6880
                     /locus_tag="VFQ61_05760"
     CDS             5912..6880
                     /locus_tag="VFQ61_05760"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET9953985.1"
                     /translation="MHGRLFGFDLSLFGADYSMPFPSYFVLLITGFLFATLMGAVWAK
                     RIGHDPDVIVDLGLAMLLSGVAGGRILHVLVDGYFWDYVHLCTDPSQVSWKVTKAQCL
                     SEYAGYWDMLAEVCRPKEQDCWAWARFYAGGLTYYGGFAGASITAFYLLRADRFPFWR
                     AADMAGMAVPIGLGFGRMGCLLAGCCFGKPSELPWALSFPSGSPASEAQHRDGLLSAV
                     GLPSLPVHPTQIYESAASLAIAAFLILYVHGRKRYDGYVFVCFVALYAAARFLIEFLR
                     NDDRGGLLALSTSQWLGLLLLGLAYAAHRRLSGSSTPVVSATEGAA"
     gene            6894..7712
                     /locus_tag="VFQ61_05765"
     CDS             6894..7712
                     /locus_tag="VFQ61_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953986.1"
                     /translation="MEGPERGWGLALAVVGALSGCVERSSHPPDAEPCPPGTPCVVPV
                     ISVRGTQVPNEGSDGEGGAKNGGEGELTGRVLEVITDSFSPSGVHTAPAWVEVLGTNG
                     LVVRAEWDGKEAFTLDGFSRSRTSWFSVRPDAGNAWLRTLQLVDTERYEASDLLLVSS
                     DVVTTALSLVSVPVTPSAIAGHALLVFYSGASPKAGVRVQVAGAELVAYASGGTWSRD
                     EDRTDTSGAVLLGNVRAAVFPGSKIEAKLSGSLTGTVTFQVAREAISLVRVQAD"
     gene            7842..8717
                     /gene="folD"
                     /locus_tag="VFQ61_05770"
     CDS             7842..8717
                     /gene="folD"
                     /locus_tag="VFQ61_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HET9953987.1"
                     /translation="MATLIDGKAIAAKVRAEVEQRVREFKAQHGRAPALHVVLVGEDP
                     ASVVYVRNKERAAHEVGMGGAVHRLPATTDEAHVLGLVRELNLSHDVDGILVQFPVPS
                     GIRQSEIVRTISPVKDVDGLTAANIGALWGGKPNLVPCTPRGCMRLLAEASVSLEGAH
                     AVVVGRSNLVGKPVAALLLAQNATVTLAHSKTRDLAQVCRNADVLVAAVGRAKMIPAS
                     FVKPGAAVIDVGMNRDEAGKLCGDVDFEAAKAVAGSITPVPGGVGPMTIAMLLENTVI
                     AAEARLSAWEQSDNL"
     gene            complement(8686..9330)
                     /gene="coaE"
                     /locus_tag="VFQ61_05775"
     CDS             complement(8686..9330)
                     /gene="coaE"
                     /locus_tag="VFQ61_05775"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236339.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET9953988.1"
                     /translation="MSLHVFALTGGIGSGKSTVSRHYRARGLPVVDADQLAREVVASG
                     QPALEDIRRDFGPELVSASGELDRTALADIVFSDPHQLARLEGLLHPRIQQLAAARFA
                     ALAERGEPLAAYEVPLLFEKGLDRFYSPVVLVSAPRDVQVARARGRDASSTQAVLARI
                     AAQMPLEAKAAKADYVIDNSGELSQTLASADRVLEALCERFGVPIRDYPIVPTR"
     gene            9386..10762
                     /locus_tag="VFQ61_05780"
     CDS             9386..10762
                     /locus_tag="VFQ61_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HET9953989.1"
                     /translation="MKLRDKLSFLPSPAREERAETGEVSYRDLAPPTAAPMPAVSPDN
                     ANESNAERGGNAARSSSAAPVAGIDSDRTRVLSDLRAKIAAVLGRPQPERVLPEPSQC
                     QLGFERVEGRRGTLYRRLERLMPSHHVGNIPVDAASQVQGSMLALLALDPSLSGLRFD
                     RALYVDTETTGLGGSGVLAFLVGLCWFDTDRRAWVEQLLLRTPGDEPALLERLHECFE
                     SCELIVSFNGKSFDLPLLQTRCVMNRMPPLPVRPHLDLLHIARRLHRKRLGVCRLTNL
                     EEAVLGFGRGPDVASADIASRYGHFLRTADEAALEGVVLHNAWDVVSMAALVGLYGEP
                     LGLLPAADLVGVARTLRRGGELEYAGRVAQTAVERGAGAEALRARGDISKARGDRAAA
                     LRDYEALLGEVQDPGVHLELVKLYEHYVKAPDRALELLDRGTGEKPEALARRRARLER
                     KRAKRSAG"
     gene            10810..>12845
                     /gene="leuS"
                     /locus_tag="VFQ61_05785"
     CDS             10810..>12845
                     /gene="leuS"
                     /locus_tag="VFQ61_05785"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019235960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET9953990.1"
                     /translation="MAYDHRRIEATWQKYWEDQQTFRAQRRPGHPKYYVLDMFPYPSG
                     SGLHVGHPEGYTATDIVARYKRMRGFDVLHPMGWDAFGLPAEQHAIQTGTHPRKTTAD
                     NIAVFRRQLKSLGFAYDWAREINTTSLDYVRCTQWIFLKLVERGLAFQSEIPVNWCPA
                     LGTVLANEEVVDGRSERGNHPVIRLPLRQWQLRITAYADRLAADLEGLDWPETREKQR
                     DWIGRSEGAEVEFALKDRADKLTVYTTRPDTLFGATYMVIAPDHPLTLQITTPDRVSE
                     VEAYAKAAARKSDLERTALGRQKTGIFTGAYAINPMNNAEIPVYTADYVLGAYGTGAI
                     MAVPAHDERDFEFAQAFGLPVIEVVSADGQLHDELERAFCDDGVAVRSGQFDGQPTAV
                     IKRNITEYLQAKGIGRRKVNFKLRDWVFSRQRYWGEPIPIYFPVRTDGDPRAGAAYEV
                     DFSTPIPVPESELPLQLPELDDYQPGDPEGPLARALDWRFFQKDGRWYARETNTMPQW
                     AGSCWYYLRYLDPQNASLPFSPESYDQWMPVDLYVGGSEHAVLHLLYARFWHKVLFDA
                     GLVKHAEPFQKLVHQGMILGIAYRYYVVTDPNAPDPKSAKVLRALDGDTPGIVRADEA
                     DGGALTLPSGEKVQERWVPEAEVEFRDGKPYQREFSVRLFPVVEKMSKSRGNVI"
BASE COUNT         2311 a         4023 c         4168 g         2343 t
ORIGIN      
        1 caacgagcgg tgccgggtag acacgaaacg cagcgtccta attcgggggc gcaggttagc
       61 ccgtcatcgg cgcactcttg ctcgagctgg tacgtgcggc ggggacccac cgcgacgcac
      121 cgctcgaggc gtgaactcac gcaacgcagc tcgccaagct cgcaagccgg cacgagctcg
      181 tcgaagtacc aacgttcgcc gggatccatg caagcgccca cgagagctga cccccacagc
      241 accagcgcta tgggaccgat gaatttcgcg cagtttcgag ccactcaata atccgaagaa
      301 gacgcgtgat ccaagaagaa cgcgtcgcgc gcatacgccg cccaagctcg gtcggtgaga
      361 tcgcggtaac gcaagctcgc gtagtcgaaa ttggggaggc gagccaacgt cagcgtcacg
      421 agctgttgcc cggacggcca cgcgatatcg gtactgatcg caccgaggtc tggcaaacgg
      481 acgcggcgca cgtttcccgg ggcgacgatc tgccaatgat agaggtcgct gttcgaggtg
      541 aggtcgacca gtgcgagatc cgcgctcggg ccacctgcca cgtgagtaaa ggcgagctca
      601 cgcccggacc actcgccgtt gggttctggt tcggtgagca ccgggatttc cagaaaggcc
      661 ccggggtcga ggacttcact cgtgtcgcgc gtcaccacgc gagccagcac tgattctggc
      721 aaagttcccg catcgcccgt caccgcgcgc agcgagacta cgtaattggc tgcagcgaga
      781 cccttcgaaa gaggtgggat tcccacgaac tcgagtgtgg agccggcgct caacaacgcc
      841 gatttttgcc cacccggcag caacacatag ccctccgtgc cgacctgtag ggccagattg
      901 ccctccacgc ggtccggccc tcggcgcgtt ggtttgggac ccgcaacgtc gatgcgcagc
      961 gcatggtcga gcccgacgtt cacttcgatg tagacgtccg ccgtttgacc gctggacagc
     1021 gcgggcacgc cgcgcaccaa acccatcgca tagggggtga acgtgaacgg ctgccgcctg
     1081 cggttctcga cgcccgccag cgcgtacaag gtgtaggtgc ccggccgggt gaagagagag
     1141 aacgatgccc ccgaaatttc gttggtgtcc ggcgtaatgg tgcccaccgc gctaggcagc
     1201 gagaaagcat cggtgggtcg ggacgccagc gcgaaaacat aggcggccag catctcgctg
     1261 tccgtcttgg gcgctggcac attgccccaa tcgtgccggc ggaactcgcc atccaaaggc
     1321 cacaccagcc cgcccgacac caccgccgta tccgaaggag tgccgccggg agcttcgaga
     1381 tcgcccttcg gatcgaaaca cgcgggtgag agcaccggat cgagatatac ggtgagccta
     1441 tcgacgggca cgtccacgaa cgttacgggt tgaaagcaac gtcgaacgac ggtgatggtt
     1501 tgtttggggc ctagctcgcg ttgctgaacc acggccacgc cgtcccgatc cgtgcgttcc
     1561 acttccgggt catcgactcc cacaatcacc gaggcaccgg gcaaggcctc gccttggata
     1621 ctgtcgaaga ccagaacggt cagctcgtcc cgcaacgggc tccctgtgaa cccgcccctg
     1681 aacccgttgt cgctgttggt gtacgcaaag ccatcgagaa cgtcgatgcg ctcttcccca
     1741 gacgtgacgc tcacgggctt tgcgccgggg ctacccgcgg gggcgcggca ggtgagctcg
     1801 cttgggctca gcagttccac gagctcacat ggctgatcgt caatctggat ctgcgtgtcg
     1861 ctatcccacg atgtcccttg cccatgcagg gtcacgagcg tgccgcctgc cgtgggcccg
     1921 gtgctcggct cgacgtagaa ggcgtcgtag gagaagccgc cgcgcagaac tacgcgcgtc
     1981 gaaacatcgt ccccattctg caccgccaca tcgactggcc ctgcctcgcc cgccggagcg
     2041 gtgacttgca gccggtgact atccacggag agcacttgct cgctcgggat ctcgttctca
     2101 ccgaaccaaa cgcgggtgtt gccggcaaag ccgttgcccc tcagcagcac caaggtgcct
     2161 cccgagaagg ggccatgcgg aggctctatg ccgagcagcg catgaggcag agcctcgggt
     2221 tcttcatcgg ggcgggtggt accagcgtcg agcgttggag ggagatcact tccgtcatct
     2281 tcgatcctgg gtagcgatgc tttgccgctt gctcggcatc cccccaagag tgaggcacca
     2341 acgaggatca ccaacgccgt ttgaagtctg aacctcatga ttgctcacat ctcagttgtc
     2401 acgaccaatc ctgaggtctg accgtcatgc ggtggagaca tgaccgtagc atccggcaag
     2461 ccgaggctcc atctctctcc ggggtccaat ctcgaggcat cgacagacca tcagtggcgg
     2521 cgtggcgcct ggcccgcgct gagccggcta aacctgcaag cgagcgagca gctgaagcgc
     2581 aagtttcttg cgcacggaat ccgaatcgga aggccctata caggccgggc agccgaagcc
     2641 gcaagcacag cgggatacga gtgtggctac gcgctcgagg agcacctcga tttgctcgta
     2701 gattcgaggg gcaaaaccca ctccgccagc gtgtgcatcg aacaagaaca gggttggatc
     2761 gaattgcccc gtatgccgag cgaaaggatc gcgttcaggg ggatgcccga ctgcggcgac
     2821 ctgcccgggc ccgcttgcgg ccagttcgct tccagcctcc cggaagtcag ccggaccgag
     2881 cccagcctgt cccagcgtag aaccgtcccc cagcgtctga cctaaatccc gcggctcgca
     2941 catcaaaaac aacgtgctca ccgtctccag cgccgagccg agtgcgcgta aagcgtcgat
     3001 tacggacgag cgcgcctcgc cgagctcctc gatgatgcgc tcgggcacgg tcaaccaaaa
     3061 gctcgtcgtg tgcatttgga tttcgggcaa gtgcacgtcg ccgtagcccg cgttctcgtg
     3121 ggtgaagaac ttgatcttct tgtagccggt gaccctcgac aagaccttca cgtcaccgtg
     3181 cccgaccagc gcccgcccaa ttgggcgggt ctgcgccgtc tccagaacgc tcacggtgcg
     3241 ataggtgaga gcggtcgtga agtagtccgg ttcgactttg cgaacgaagg ccttgtgatt
     3301 gtcgaaatcc aaacgttcga cctgatactg ctcggcgtcg tgctgataga tcgcctgctc
     3361 gtgcaacatg gtgtgcgccg cccggtagtc gagctccgcc agtgatttgc ccgtaccgac
     3421 gtcgatgatt acgaagttgt cccagccgat gttgcggagg gacacattgc tcgccggaaa
     3481 agcctccgcg gcaaagtggt agcgcccgtc cgaagcgtga actactccga ggctcgccag
     3541 gtaatcgagc gcatcccgcg tggactccag atccagcccc aagtgccgct cgccctgcgc
     3601 cggtgccgga tccgcgggtt tcgaacctcg cgcgctgacc tcgtaggggg cctcgaacac
     3661 cgcacacttc aggtgttgta acgatatttc gaggttcgaa ggatcgatgc gcgcctcctc
     3721 cgcacccgct ctaaacagat attctggatc gctcgcaagg tactgatcca ggcccgagct
     3781 agaacacacc aacagagcca cgctccggtc tccccgccgg cccgctcgcc cgaagcgctg
     3841 ccacgtggcg gcgatggaac cgggatagcc agcgcatacc accgcgtcga gatctccgat
     3901 gtcgatgccg agttcgagcg cattggtcgc aacgacgcac aatatttcgc cgttgcgcag
     3961 gcccgcttcg actcgccgcc gggtctccgg caaatagccg ccgcgataac ccatgatggc
     4021 ttctggcccg gccacattgc ccacgcgcgc acgaaggtac ttgagcatca cctcgacgga
     4081 gttacgggat ggtccgaaca ccagagtcgg cactcgcgcc tcgaccaagt ccgccgctag
     4141 agacacgctg tgtttgagcg tgctgccccg gatccccaga tccgcgttca cgatcggtgg
     4201 attatagacg taaacctcgc gcgctgccgc gggggctccc gaacgatcca ccagcgcgat
     4261 ctcctctggc ttgcaatcga agaggcgcgc ggcgtgttca cgcgggttgc cgatggtggc
     4321 cgtggccgcc aaaaacgtcg gctgtttacc gtgaaatgcg gcgacccgcc gaagccgagc
     4381 gagcacgtgc gccatgtgcg aaccgaacac tccgcgatag gtgtgcagct cgtccagcac
     4441 cacgtaggtg agcccctcga acagcgaagc ccagcgcgcg tggctcggca gaatgccgga
     4501 gtgaagcatg tctgggttgg tgagcaccac tcggcaacgc tcacgcgccg cccgccgcgc
     4561 gtctccgggt gtatccccgt cgtagaccat ggcctgcgtg ggcaggccgg cgtccgccaa
     4621 tagcccgagt aagccatgtt cttgatcgcg cgacagcgcc ttggttggat agagatagag
     4681 agccgtcgct tttggatcgc gcgccagccg ctccagcacc gggatgtgga aacacaagct
     4741 cttgccgctg gccgtggggg tcgccacgac cacgtgtttc ggctcgagcg ccgcccggat
     4801 ggcctgcact tggtgcgagt agagctgccg gatgttgcgc cccgcgagcg cccgacgcgc
     4861 ggcttctgac agctcatccg gcagcggctc gaacactgct tcccgtgccg gcagcgcccg
     4921 ctcggcggcc aggcaacgtt tgacgctgcc cgcatcttgc caagcatcga cgacggcgcc
     4981 cacaccaacg gagcgcttcc agggcgggtc gggcatggcc ggcatgctaa acaaatgttc
     5041 ggtatttttc aaggcgacag ccaaatggcc ctggctggat tttgcgagct gtcgtagtgc
     5101 gtagggcagc caatgagtga atctgcttcg cccgatgccc aagatctatc ggccgcaccc
     5161 ggagcgccga cccccgcggc gcccgagccg ctaacgaacg ccgcttcggt gagcgccgct
     5221 tcgaagagtg gagcggaacc gcatgccggc gtgggcttgg cagagggccc caagcgttcg
     5281 cggccgcgtt tcgtgttctt tgggatcgtt tccgccgtga cgctgttgct cgacatcgcg
     5341 tccaaggctt gggcggaggt cacgctcact tcgcgtccag cacaccagcc ctcgatcacg
     5401 gtcatcgcgg atcatttgtc gttcacgctg gcgtacaatc gcggtggcgc gtgggggttg
     5461 ctgcaagacg ctcacgagtc cattcggctg ctgttctttt tgacggtgaa catcgtggct
     5521 ttgctgttca tcacttggct ttacagcaag ctcgtgcgag gccagcgcgc gctgatgtgg
     5581 ggcttgccgc tcgtgatggg gggagcgctc gggaatttct ccgatcgcat cactcggtcc
     5641 agcgtcatcg acttcatcga ctaccgcgcg cagtgggtgt tggcgatgaa tcggctcatc
     5701 ggcaaggtct cgcccaactg gacgatcacc gatcactggc cgacgttcaa cgtggcggac
     5761 atcgcgattt gcgtgggcgt gggcttgatt ggcttggata tgttcacgtc caaacacggc
     5821 ccagttcagc aggatccggg ctctccgctc cagccggggg ccaatcggga atcttcgagc
     5881 tcggaaacgg caccgagcgc ctgatcgagc catgcacggg cgtctgttcg gattcgatct
     5941 cagcttattc ggggcggact acagtatgcc gttcccgagc tacttcgtcc tgctcattac
     6001 cggtttcctg ttcgccactc taatgggcgc ggtgtgggcg aagcgcattg gccacgatcc
     6061 cgacgtgatc gtcgatctcg gtctagccat gttgctttcg ggcgtggccg gcgggcgcat
     6121 cctacacgtg ctcgtcgacg gctatttctg ggactacgtg catctgtgca ccgacccgag
     6181 tcaggtctcg tggaaagtga ccaaggcgca atgcctcagc gaatacgcgg gctattggga
     6241 catgctggcc gaggtgtgcc gccccaagga gcaggattgt tgggcttggg cgcgcttcta
     6301 tgctggcggc cttacttact acgggggctt cgccggggca tcgatcacgg cgttttatct
     6361 acttcgagct gatcggttcc ctttttggcg agccgccgat atggcgggca tggccgtgcc
     6421 aatcgggctg ggctttggga gaatgggttg tctgctcgcg ggctgttgct tcggtaagcc
     6481 gtcggagctg ccgtgggcgc tgagtttccc gtccggtagc cccgcgagcg aagcccagca
     6541 tcgcgatggg ctgctgtctg cggtgggctt gccttcgttg ccggtgcatc cgacccagat
     6601 ttatgagtcc gcggccagct tggccatcgc ggcgttcctg attttgtacg tgcatgggcg
     6661 caaacgctac gatggctacg tgttcgtttg cttcgtggcg ctctacgcag ccgcacgttt
     6721 cttgatcgag ttcttgcgca acgacgatcg cggcgggctg ctcgcgctct ccacctcgca
     6781 gtggctcggg ctgctgttgc tcgggctcgc gtatgcggcg caccgtcggc tttcgggatc
     6841 gtcgactccg gtcgtgtccg ctacagaagg ggcagcttga tatgaggtgg ggagtggaag
     6901 ggccggaacg cgggtggggg ctagctttag cggtggtcgg cgcgctttcg ggctgtgtcg
     6961 agcgtagttc tcacccgccc gacgcggagc cttgtccgcc gggcactccg tgcgtggtgc
     7021 ccgtaatcag cgtgcgggga acccaagttc cgaacgaagg ctcggacggg gaaggcggcg
     7081 ccaagaacgg cggagaaggg gagctcacgg gccgcgtgct cgaagtgatc acggactcgt
     7141 tttcaccctc gggagtacat accgcaccag cttgggtcga ggttttgggc acgaatggtc
     7201 tcgtcgtacg cgcagaatgg gacggcaaag aggcattcac gctggacggt ttctctcgca
     7261 gtcgcacgag ctggttcagc gtacgccccg acgcgggtaa tgcctggttg cgcaccctcc
     7321 agctcgtaga cacggagcgc tacgaggcct ccgacctgct gctcgtctcg agtgacgtgg
     7381 tcactacggc gctgtctctc gtgtcggttc ccgtaacccc ttcggcgatc gcggggcacg
     7441 cccttttggt gttctactcg ggagcttcgc cgaaggccgg cgtgcgtgtg caggttgcgg
     7501 gggccgagct cgtcgcgtac gcgagcggcg gaacttggtc ccgagacgag gaccgaaccg
     7561 acacgagcgg ggcggtgctc ctagggaatg tgcgtgccgc ggtctttccg ggcagcaaaa
     7621 tcgaggcgaa gctgagcggc agcctgacgg gaaccgtaac cttccaggtc gcccgcgaag
     7681 ccatctcact ggttcgcgtg caggccgact gagacgcggg gaacccgcct cgaggttccg
     7741 atccaacgag ccccccaatt cgatggagat tgcttggctt gtacttgggg ccgtcgcctc
     7801 cacatggacg ctcgtgacag actctgctat ccagccgccc cgtggcgacg ctgattgacg
     7861 gcaaggcgat tgcggcgaag gttcgagcgg aagtggagca gcgcgtgcgc gaattcaaag
     7921 cgcagcacgg gcgagctccc gccctgcacg tggtgctggt aggcgaagac cccgcgtcgg
     7981 tcgtctacgt ccgcaacaag gaacgagcag cccacgaggt gggcatgggc ggggcggtac
     8041 atcgtctccc ggcgacgacc gacgaagctc atgtgcttgg gctcgtgcgt gagctcaatc
     8101 tttcccacga cgtcgacgga attctggtgc agttccccgt gcccagtggc atccgtcaaa
     8161 gcgagatcgt gcgcacgatc tcgcccgtga aagacgtgga cggcctgact gcagccaata
     8221 tcggcgcgct ctggggtggc aagccaaacc tggtgccctg cactccacgc ggctgcatgc
     8281 gactgctggc cgaggcgtcc gtatcactcg aaggcgctca cgcggtcgtg gtcggccgca
     8341 gcaacctggt cggtaagccg gtggccgcgc tgctgctagc gcagaacgct acggtcacgc
     8401 tggcccactc caaaaccaga gatctggctc aggtttgccg gaatgcggac gtgctggtcg
     8461 ccgccgtggg gcgcgccaag atgattccgg cctccttcgt gaagcctggc gcagcggtga
     8521 tcgacgtcgg aatgaaccgt gatgaggcgg ggaagctgtg cggcgacgtc gacttcgaag
     8581 ctgcaaaagc ggttgcgggc tcaatcactc ccgtgcccgg cggcgtaggg ccgatgacca
     8641 tcgccatgct cctcgagaat acggtgattg ccgccgaggc ccggctcagc gcgtgggaac
     8701 aatcggataa tctctgattg gaacgccgaa tctctcacac agagcttcga gcacccggtc
     8761 tgcgctggcc aaggtttggc ttagttctcc cgaattgtcg atgacgtagt cggctttggc
     8821 ggccttggct tcgagcggca tttgggcggc gatacgcgcc aagacggcct gggtactgct
     8881 agcgtcgcga ccccgcgcgc gcgccacttg tacatcacgt ggagcactga ccagcacgac
     8941 ggggctgtag aatctatcta atcccttctc gaacagcaga ggcacttcgt aggcggccaa
     9001 gggctcaccc cgctcggcca gtgctgcgaa gcgtgccgcc gccagctgct gaatgcgcgg
     9061 atgaagtagt ccttcgagcc gagccaactg gtgggggtcc gagaacacga tgtcggccag
     9121 cgcggtgcgg tcgagttccc cggatgcgga tacgagctcg gggccaaagt cacgccggat
     9181 gtcttcgagt gcaggttgcc ccgaagcaac cacctcgcgt gcaagctggt ctgcgtctac
     9241 gacgggcaac ccgcgagcgc gataatgacg cgaaacagta ctctttccgg acccgatccc
     9301 gccggtcagc gcaaagacgt gcaggctcac gaagcaagct tacacgccaa gatgtggtaa
     9361 cgcatccacg ccggttcggg cgcccatgaa gctacgcgac aaactctcgt tcctgccgtc
     9421 gcccgcgcgt gaggagcgag ccgagacggg agaggtgagc tatcgagacc ttgctccgcc
     9481 tacggcggcg cccatgccgg cggtgagccc cgacaacgca aacgagagca acgcggagcg
     9541 cgggggtaac gcggctcgct cgtcttccgc ggcgccggtc gctggcatcg acagcgaccg
     9601 cacccgtgtt ctgagcgatc tacgcgcgaa gatcgcggcc gtgcttggtc gacctcaacc
     9661 cgagcgagtg ctgcccgaac ccagtcagtg tcagcttggg ttcgagcgcg tcgaggggcg
     9721 tcgggggacg ttgtaccgac ggctcgagcg gctcatgccc tcgcatcacg ttggcaacat
     9781 cccggtagac gccgcgagcc aggttcaggg gtccatgttg gcgctcttgg cgttggatcc
     9841 aagcctgtcg gggctccgat tcgaccgcgc actctacgta gataccgaga cgactgggct
     9901 cggtggctcg ggcgtgctcg cgttcttggt gggcctatgc tggttcgaca ccgatcggcg
     9961 cgcctgggtc gagcagcttc tcctgcgcac gccgggggac gaacccgccc tgctcgaacg
    10021 tttgcacgag tgcttcgagt cttgtgagct gatcgtctct ttcaacggca agtccttcga
    10081 cctgccgctg ctccaaacgc gctgcgtgat gaatcgcatg ccaccgctgc cggtcaggcc
    10141 gcacttggat ttgctgcaca ttgctcgtcg actgcaccgg aagcggctcg gcgtttgccg
    10201 tttgaccaac ctggaagagg ccgtgctcgg cttcggtcgt ggcccggatg tagcatcggc
    10261 tgacatcgcc tctcgttacg ggcacttctt gaggactgcg gacgaagcag ccctcgaagg
    10321 ggtggtactc cacaacgcat gggacgtggt tagcatggcg gctctcgtcg gcttgtacgg
    10381 agagccgctc gggctactac ccgcggccga tctggtgggc gtcgctcgaa cgttgcggcg
    10441 cggtggggaa ctcgaatatg ccggacgggt ggcgcaaacc gccgtggagc gtggagcggg
    10501 cgcggaagcc ttgcgcgcgc gcggcgatat ttccaaggcc cgcggtgatc gcgcagccgc
    10561 gttacgcgat tacgaggcgc tgctcggtga agtccaggat ccgggggttc acctggaatt
    10621 ggtaaagctt tacgagcatt acgtgaaagc gccggaccgc gctctcgagc tgctcgaccg
    10681 agggaccggc gaaaagcccg aagccttggc cagacgtcgc gcccgtttgg agcgcaagcg
    10741 cgcgaaacgt tcagccgggt agttggcgaa gttggcgcaa ttcgcgagtc ttctgggcta
    10801 aagtaggcca tggcttacga tcaccgtcgc atcgaagcga cgtggcaaaa atactgggaa
    10861 gaccagcaga ctttccgcgc gcagcgcaga ccaggccatc cgaagtacta cgtgctcgac
    10921 atgtttccgt acccttcggg atccgggctg cacgtgggtc accccgaagg ctatacggcg
    10981 accgacatcg ttgcgcgcta caagagaatg cgcgggttcg acgtgctgca tcccatgggg
    11041 tgggatgcat tcgggctgcc cgcggagcag cacgccattc aaacgggaac gcacccgcgc
    11101 aagacgacgg cggacaacat cgcggtgttt cgccgacagc tcaagagcct cggtttcgcc
    11161 tacgactggg cgcgcgagat caacaccacc tcgctggatt acgtgcgctg cacgcagtgg
    11221 atcttcctga agctggtgga gcggggtctc gcgtttcagt ccgaaatccc ggtaaactgg
    11281 tgccccgcgt tgggtacggt gctcgcgaac gaagaagtgg tggatggtcg tagcgagcgc
    11341 ggcaaccacc ccgtgatccg cttgccgctg cgccagtggc agctgcgaat caccgcctac
    11401 gccgaccgtc tcgcggcaga cctggagggc ctggattggc cggagacgcg cgaaaagcaa
    11461 cgcgattgga tcggtcgcag tgagggcgcc gaggtggagt tcgcgctcaa ggatcgcgct
    11521 gacaaactga cggtctacac cacgcgacca gacacgctgt tcggtgcgac gtacatggtg
    11581 atcgcgcccg atcacccgct caccctccag atcacgacac cagaccgcgt ttccgaggtc
    11641 gaagcttacg ccaaggcggc cgctcgcaag agcgacttgg agcgcacggc gctcgggcgc
    11701 cagaagaccg gcatcttcac cggcgcgtac gccatcaacc cgatgaacaa cgccgagatc
    11761 ccggtgtata cggcggacta cgtgctgggt gcgtacggaa cgggcgcgat catggccgta
    11821 cctgcgcacg acgaacggga cttcgagttt gcccaagcgt tcgggcttcc cgtgatcgaa
    11881 gtcgtaagcg cagacgggca gctgcacgac gagttggaac gggcattctg tgacgatggc
    11941 gtagccgtcc gctccggaca gttcgacggg cagcccaccg cggtgatcaa gcgcaatatc
    12001 accgagtatc tgcaggccaa aggcatcggg cggcgtaagg tgaatttcaa gctgcgcgat
    12061 tgggtcttct cgcgtcagcg ctactggggc gagccgattc caatctactt ccccgtgcgc
    12121 actgacgggg atccccgcgc tggcgcggcg tacgaggtcg acttctcgac gccaatcccg
    12181 gtgcccgagt cagagctgcc gctgcagctg cccgaactgg atgactatca gccgggagat
    12241 cccgaagggc ccttggcgcg ggcgctggac tggcgctttt tccagaagga tgggcgctgg
    12301 tacgcgcgcg agacgaatac gatgccgcag tgggcgggct cgtgttggta ctacctgagg
    12361 tatctcgatc cgcagaacgc gagcctgccg ttctcacccg aaagctacga tcaatggatg
    12421 ccagtcgatt tgtacgtcgg cggctccgag cacgcggtgt tgcatctgtt gtacgcgcgt
    12481 ttctggcaca aggtgctgtt cgacgcgggc ttggtgaagc acgccgagcc attccagaag
    12541 ctggtgcacc agggcatgat cctagggatc gcgtatcgct attacgtggt gacggatccc
    12601 aacgctcccg atccgaagtc ggccaaggtt cttcgagctc tggacggcga caccccgggt
    12661 atcgtgcgag cggacgaggc cgacggggga gccctgactc tgccaagcgg tgaaaaggtt
    12721 caggagcgct gggtgcccga agcagaggtc gagttccgcg atggtaagcc gtaccagcga
    12781 gagttctcgg tgcggctgtt cccagtcgtg gagaagatga gcaaatcgcg gggcaacgtg
    12841 atcaa
//
LOCUS       DASTKU010000161         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1144763, whole genome shotgun sequence.
ACCESSION   DASTKU010000161 DASTKU010000000
VERSION     DASTKU010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144763"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFQ61_05790"
     CDS             complement(<1..277)
                     /locus_tag="VFQ61_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953991.1"
                     /translation="MQNPREAETPPQTASATRTPTSGDRGSWPALAVTLTYWALSYGF
                     TRFRDIFEIDPDEGNNLIKALLLDRGHAFLTEIWSDQPPLFPHLLWGA"
     gene            complement(544..1860)
                     /locus_tag="VFQ61_05795"
     CDS             complement(544..1860)
                     /locus_tag="VFQ61_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET9953992.1"
                     /translation="MADRADSPSKDEPSSGAIPGPPLSRAGRDLSVNRSPSTEKHARA
                     RGIRNWVRRGVYVVVALVVGTLIVIAVLPKPIPVERASVTRGVLRVTVDEVGRARVRD
                     RYIISAPLAGNVSRAELRAGDRIRPGTVLARIAPLEAPMLDPRSRTQAEARVLAAAAS
                     ERQARSAVLRAKLSLEQEKTEADQNRKLAESGSLSPNALSRSILSERLRKEELASAEF
                     AAQMAAYEVAQARAMLARSEPSKALDQIEVVSPISGRVLSVLRESAGVIQAGTQLLEI
                     GDPGALELVSDVLTSDAVSINSGAPAKVVRWGGADLDAHVRLIEPSAFTRISALGVEE
                     QRVHVILDLDAPRASWESLGDGYRVEVRIVTWEVHDATLVPASALFRSGSGFAVFGIE
                     QGRARIRQVELGRSNGIETEVIRGLRAGDPVVLHPSDRVSDGVRVE"
     gene            complement(1862..>2784)
                     /locus_tag="VFQ61_05800"
     CDS             complement(1862..>2784)
                     /locus_tag="VFQ61_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9953993.1"
                     /translation="STSARNELLALPGVLQVEPELGIPVKLRANQHVRDVVLEGRRPE
                     ATLQRIVAFPLKIVPVPERGAVISEELARVLEVKPGQTLRIEPYAGDRRARELLVAGS
                     VADTFGLRVHMSLESLWRTFDEWGVMSSALLRVDGRRETELLSRLSDTPMVLSVTRRQ
                     AIIDRFRQQTADNMYAVTFVLTAFAATIAVGVVYNNARIALALRARDLSSLRVLGFTR
                     AEISRVLLGELFVQVFVALPLGLGIGVRLSHLLFSSMDRELYRFPVVISGRTYAFACL
                     VTLLAAALSALLVRRRLDRLDLVAVLKTRE"
BASE COUNT          556 a          942 c          839 g          447 t
ORIGIN      
        1 aggctcccca caacaggtgc gggaaaagcg gaggttgatc gctccaaatc tcggtcaaga
       61 acgcatgccc tcgatcgagt aatagcgcct tgatcagatt gttgccttca tccggatcaa
      121 tctcgaagat gtcccggaat cgggtgaagc cgtaggaaag cgcccaatag gtcagagtga
      181 cggcgagcgc aggccacgag cctcgatcac cggacgttgg tgtgcgcgtc gctgaggctg
      241 tttgcggggg cgtttccgcc tctcgaggat tctgcatgga gggcaagcca agaacttcgg
      301 cgagagccca cttccgggcg ctccgtccga tacgccaaac aagacgatca ttgtacctga
      361 tacgacgctg accggaagcg ccctctcgag gtaggcgcac ccagcctgca agcagaagtg
      421 ggtaatcagg ctcttggtct ctgattgcgg gccgcggaac gccggcggaa tcggcccatt
      481 tttgctaatg ggaaaatctc agctcgagga gcccgacccc aagacggcgc gtagcgaaag
      541 tcgtcactcg actcgtacac cgtcgctgac ccgatcgcta gggtggagca cgaccggatc
      601 ccccgcacgc agaccacgta tcacctcggt ttcgattccg ttactgcgcc cgagctcgac
      661 ctggcgaatg cgcgcccggc cctgttcgat cccgaagact gcgaacccgc tgccactccg
      721 gaacaacgcc gaagcgggca ccaaggtcgc atcgtgaact tcccaagtca cgatgcggac
      781 ctcgacgcgg tagccatctc cgagtgattc ccagctcgca cgcggcgcgt ccaagtctag
      841 gatcacgtgt acccgttgct cttcgactcc gagcgccgaa atccgagtga acgccgaagg
      901 ttcgatgagc cgaacgtgtg cgtccaaatc tgcgccgccc cagcgcacga ccttggccgg
      961 cgcgcccgag ttgatggaca ccgcgtcgct cgtgaggaca tcgctcacga gctcgagcgc
     1021 ccccggatct ccgatttcga gcagctgagt tcccgcttga atcacccccg cactctcgcg
     1081 cagcacgctc aaaacccggc ccgagatcgg cgacacgacc tcgatttgat cgagcgcctt
     1141 cgacggttcg cttcgcgcca gcatcgcgcg cgcttgcgcg acttcgtacg cggccatctg
     1201 ggccgcgaac tcggccgaag cgagctcttc tttacgcagc cgctcggaaa ggatcgagcg
     1261 agacaaggcg ttgggcgaaa ggctaccaga ctcggccaac ttgcggtttt ggtcagcctc
     1321 tgtcttctcc tgttcgagcg aaagcttggc gcgcagcacc gccgaccggg cctgtctttc
     1381 gctcgccgcg gccgcgagca cgcgcgcctc ggcttgcgtg cgcgagcggg ggtccagcat
     1441 gggggcctcg agcggcgcga tgcgcgccaa caccgtaccc ggccgaatgc ggtcacccgc
     1501 tcgtaactcc gcacgtgaca cgttgccggc caagggagca gaaatgatgt agcgatctcg
     1561 aacccgagcc cgtccgacct cgtccacggt cacgcgcaac acgccccggg tgactgatgc
     1621 gcgttcgact gggatgggct tgggtaagac cgcgatgacg atcaacgttc ccaccaccaa
     1681 cgcgaccacc acgtaaaccc cgcggcgcac ccaatttcga attccgcgcg cccgcgcgtg
     1741 cttttccgtc gaggggctgc ggttgacgct caagtctcgc cccgcacgcg agaggggagg
     1801 ccccgggatc gcccccgacg aaggctcgtc ttttgacgga gaatcagcgc ggtctgccat
     1861 gtcattcccg tgttttgagc actgcgacca agtccaaacg atcgaggcga cgacgaacga
     1921 gcaacgcact cagggccgcc gccagcagcg tgaccagaca agcgaacgca taggtacgcc
     1981 ccgagatcac caccggaaac cgataaagtt cgcgatccat gctcgagaag agcaaatgcg
     2041 agagccgcac gccaatcccc aagccgagcg gcagcgccac gaagacctgc acgaacagct
     2101 cgcccagcag caccctagag atctcggcgc gcgtgaagcc gagcacgcgc aaactcgaga
     2161 ggtcgcgcgc gcggagagcc aacgcgattc gggcgttgtt gtacaccacg ccgaccgcga
     2221 tcgtggcggc aaacgcggtc agaacgaacg tcacggcgta catgttatcg gccgtctgct
     2281 gacgaaaacg atcgatgatc gcctggcgtc gcgtcacgct gagcaccatc ggggtatcgc
     2341 tcagccgact caagagctcc gtctcgcgtc gcccgtcgac gcgcaacagc gccgaggaca
     2401 ttacccccca ctcgtcgaac gttcgccaca gcgactcgag cgacatatga acgcgcaaac
     2461 cgaacgtatc ggctacgctg cccgcgacga gtagctcgcg agcacggcga tcacccgcgt
     2521 agggttcgat gcggagcgtc tgccccggct ttacctcgag cacgcgcgcg agctcttcgg
     2581 agatgaccgc gcctcgttcg ggcaccggca cgattttcag agggaaggct acgatgcgct
     2641 gcaacgtggc ttcgggccgc cggccctcca agaccacgtc acgcacgtgc tgattggcgc
     2701 gcagcttgac cgggatgccc agctccggct ccacctgcaa cacgcccggc aacgcgagca
     2761 actcgttgcg tgcgctggtg gata
//
LOCUS       DASTKU010000162         5891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_921673, whole genome shotgun sequence.
ACCESSION   DASTKU010000162 DASTKU010000000
VERSION     DASTKU010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921673"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VFQ61_05805"
     CDS             <1..236
                     /locus_tag="VFQ61_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953994.1"
                     /translation="AQAAADQRIVQSKRAVEIETLNAQAEVEPLLALAEQLRELAGAG
                     GPNAVAAYVKNQKLPLYRRARSLVLGETQEASR"
     gene            233..1114
                     /locus_tag="VFQ61_05810"
     CDS             233..1114
                     /locus_tag="VFQ61_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET9953995.1"
                     /translation="MSEFFTAFLVAFLGLFVFVPFALGVARAFALYVVVQERQCVVFE
                     LFGKVRLVIDRPGLHCPWLQMGPFAMLVPLFGKKLVVDMRLDQTYLRSQPVNSEEGAP
                     MGIGVWCEMVVGDPLAYLYKNIDPAGSLRANISNATVRCLSNMKLADMLENRHAMSGV
                     VRHEVSAKSQEWGYHVGSVYVRKVHFRDRGMIQQIEQKVVNRLRQVTAAIQQDGTNRV
                     NVIRSAAEREAAVEFAKAAATRPRIVGAALAQIAQSPAISAALFEVLETQNLLQSQHV
                     QVDVVPKNLPMMFPLDR"
     gene            1131..1940
                     /locus_tag="VFQ61_05815"
     CDS             1131..1940
                     /locus_tag="VFQ61_05815"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9953996.1"
                     /translation="MKIVRFIDSSGNVQLAQLRSDGVHLRIEGDLFGGFQVTDESVAP
                     GRYLCPLPVKQLLCIGLNYRKHAEETGAKLPQYPVLFMKGLNAVQSPGAPIVLPRVLR
                     SDAVDYECELAVVIGKPCRNVKRDRALEYVLGYTCANDVSARDWQKHYGGGQWCRGKT
                     FDTFAPLGPHLVTRDEVPNPNALTISTFLNGERVQHSNTADMIFDVPQLIEFLSGSTT
                     LLPGTVILTGTPEGVGMARTPPTYLKAGDRVRVEIEQLGVLENPVIEETDG"
     gene            complement(1918..2406)
                     /locus_tag="VFQ61_05820"
     CDS             complement(1918..2406)
                     /locus_tag="VFQ61_05820"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HET9953997.1"
                     /translation="MTASVRASMRERLSEYAEAHRSKRNELCHFVGVPLIVFGAARLL
                     GAVRLANLAGHEISLTHALVPGLLAYYLVQARWLGLCTAALIMLLSGAGLAVPFGVGC
                     AAFVFGWLVQFIGHAHYEHRKPAFMQNLVHLVVGPAWLVERAFWSDVRHDSRVSSHPS
                     LR"
     gene            complement(2432..4411)
                     /locus_tag="VFQ61_05825"
     CDS             complement(2432..4411)
                     /locus_tag="VFQ61_05825"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HET9953998.1"
                     /translation="MSRRVVAEGELLWAPSLNRVRSCRMSDYIGWLRHTRGYVFDDYP
                     SLHAFSTQNLPCFWGSIWDYFGVRSESTVSERVRGTMPHVQWFDGARLNYAAHALSRR
                     DGRDALIFRSESEPRQVLSRSELAHAVARARAGLRRLGVGVGDRVAALVTNRPEAVIG
                     FLACASLGAIWSSCSPEFGASSVLDRFQQIAPKVLLASSSYVYGGKRFDRSAELARVR
                     AGLPSLAATVHVGEAREGMLGWDELLAEEEPLEFEPVGFEHPLWILYSSGTTGLPKAI
                     VHGHGGMLLEHLKALALHLNLDQDDRFFWFTTTGWMMWNFLISGLLLGTTVVLYDGSP
                     AYPDLNALFRLVEEESITYFGSSAPHLLACKKAGIVPARAHKLSSLKGLGSTGAPLSA
                     EGFAWVYENVHRDLALGSLSGGTDVCTAFVLPCPLLPVHAGEIQCAGLGAAVEAFDET
                     GCPVVDEVGELVLTEPMPCMPVSFWNDPNDKRRIKSYFDSYAGVWRHGDWIKQTSRGS
                     FVIYGRSDATLNRGGVRMGTAEFYRVVESLPEFVDSLVVDTSALGDGAEGAEAGKLWL
                     FVVLAPGAQLDDALKGRLRERIRQDLSPRHVPDEILAVSVVPRTLNGKKMEIPVKGIL
                     QGQPPDKVAAPGTLQDPHAFDFFVQLAQASNAAAR"
     gene            complement(4408..5622)
                     /locus_tag="VFQ61_05830"
     CDS             complement(4408..5622)
                     /locus_tag="VFQ61_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9953999.1"
                     /translation="MTAATRVNGSGSRFGPWLFGARVDLAVFTGSALVALLLSAAASA
                     LGVTEVSDWAWLALVLAIDVAHVHATWFRTYFDVSELRRHPARYALVPLLAYIGGVCA
                     YRAGMFWRVVAYLAVFHFVRQQVGWVALYRARAGLRGSLERCVDEAAVYAATLYPLIV
                     WHARSSEKHFSWLSPGDFVALDLERILPASTVLWALALASFFARELRRLWLGQAMAVG
                     RVLLVLSTAVTWYVGIVVNESDFVFTATNVIPHGVPYVWFLFAYTRERREQAPAPAAL
                     RVAGQSVAAFVCALWVLAWVEEWAWDRWVDHDRVWLFGGHTAGEGPLTQWIAWLVPLL
                     ALPQVTHYLLDGLLWRRGARAEKSALQRALGFGAHAGGAPSRVVDLAAGAGTDAGGAH
                     EGYAGRVGGVHS"
     gene            5660..>5891
                     /locus_tag="VFQ61_05835"
     CDS             5660..>5891
                     /locus_tag="VFQ61_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954000.1"
                     /translation="MELVARAERLLGAPAVYGRETRHVPPPRGVACRVGSARRAGAAW
                     SNPSRRSRLTSQQRRRHEALLARGARHNILALP"
BASE COUNT         1164 a         2020 c         1788 g          919 t
ORIGIN      
        1 tggcccaagc ggccgcggat cagcgcatcg tgcagtccaa gcgcgcggtg gagatcgaga
       61 ctttgaacgc ccaagccgag gtcgaaccgt tgctcgcgct ggccgaacag ctgcgggagc
      121 tggctggtgc cggtgggccc aacgcggtcg cggcctacgt caagaatcag aagctgccgc
      181 tgtaccgtcg cgcccgcagc ctagtgctcg gagaaaccca ggaggccagt cgatgagcga
      241 gttcttcacc gccttcttgg tggcattctt gggcttgttc gtgttcgtgc cgttcgcgtt
      301 gggcgtggcg cgggcattcg cgctgtatgt cgtggttcag gagcggcagt gcgtggtgtt
      361 cgagctcttc ggcaaggtgc ggttggtgat cgatcgaccg ggcctgcact gcccctggct
      421 gcagatgggc ccgttcgcca tgctggtgcc gctgttcggc aagaagctgg tggtcgatat
      481 gcggctcgat caaacctacc tgcgcagcca gcccgtgaac tccgaggagg gcgcgccgat
      541 ggggatcggg gtgtggtgcg agatggtggt cggcgatccg ctggcctatc tctacaagaa
      601 catcgatccg gcgggctcgt tgcgcgcgaa catatcgaac gcgaccgtgc gctgcttgag
      661 caacatgaag ctcgccgaca tgctcgagaa tcggcacgcc atgagcggcg tcgtgcgcca
      721 cgaagtctcc gccaaatcgc aagaatgggg ctatcacgtg ggcagcgtct acgtccgtaa
      781 ggttcacttt cgtgaccgcg gcatgatcca gcaaatcgag cagaaggtgg tgaaccggct
      841 gcgccaggtg acggcggcca ttcagcaaga tggcaccaac cgcgtgaacg tgatccgctc
      901 cgccgcggag cgggaggcgg ccgtggagtt cgccaaagcc gccgccacgc ggccgcgcat
      961 cgtgggcgcg gccttggctc aaatcgcaca atcgcccgcc atctcggccg cgctgttcga
     1021 ggtgctcgag acccagaact tgttacaaag tcagcacgta caagtggacg tcgtccccaa
     1081 gaatctgcct atgatgttcc ccctcgatcg ttgaaacact agagctcgtc atgaagatcg
     1141 ttcgcttcat tgattcctcc ggcaacgtgc aattggcgca gcttcgctcg gacggcgtgc
     1201 acctgcggat cgagggtgac ctgttcggtg gattccaggt caccgacgag agcgtggcgc
     1261 cgggccgcta cttgtgcccg ctgccagtca agcagctgct gtgcatcggc ttgaactacc
     1321 gcaagcacgc cgaggagacc ggcgcgaagc ttcctcagta tccggtgctg ttcatgaagg
     1381 gcttgaacgc cgtgcaatca cccggtgccc cgatcgtgct gccgcgcgtg ctacgcagtg
     1441 atgcggtgga ctacgagtgc gagcttgcgg tcgtgatcgg gaaaccctgc cgcaacgtga
     1501 agcgcgatcg cgcgctcgag tacgtgctcg gttacacctg cgcgaacgac gtgtcggcgc
     1561 gggattggca aaagcactac gggggcggcc agtggtgccg tggcaagacc ttcgacacgt
     1621 tcgctccact cgggccgcac ctcgtcaccc gggacgaggt gccgaatccg aacgcactga
     1681 cgatctccac gttcttgaat ggagagcgcg tgcagcactc caacaccgcg gacatgatct
     1741 tcgacgtgcc ccagctgatc gagttcctga gcggcagcac gacgttgttg cccggcaccg
     1801 tcatcctgac cggcacgccc gagggcgtgg gaatggcgcg tactccacct acttatctca
     1861 aggccggcga tcgggtgcgc gtcgaaatcg agcagctcgg cgtgctcgaa aaccccgtca
     1921 tcgaagagac ggatggctag agacgcgaga atcgtgacgc acgtcgctcc agaaggcgcg
     1981 ctccacgagc caagccgggc caaccaccag gtgcactaga ttctgcatga acgccggctt
     2041 gcggtgctcg tagtgtgcgt gtccgatgaa ttgcaccagc cagccgaaga cgaatgcggc
     2101 gcagcccacg ccgaaaggca ccgcgagacc agcccccgag agcagcatga tgagcgcggc
     2161 cgtacacagc cctagccaac gagcttggac gaggtagtac gcgagcaatc ccggcacgag
     2221 ggcgtgggtc agtgagatct cgtgcccggc tagattcgcg agccgcaccg cgccaagcaa
     2281 gcgcgccgcc ccgaacacga tcaatggcac ccccacgaaa tggcagagct cgtttcgttt
     2341 gctgcggtga gcctcggcat actccgagag gcgctctcgc atcgacgctc gcacgctcgc
     2401 ggtcatgccc gtaacgtagc acccgatacg ctcaacgagc cgcggcgttg gacgcctgcg
     2461 ctagctgcac gaagaagtcg aaggcatgtg gatcctggag cgtgcctggc gcggctactt
     2521 tgtccggcgg ctggccttga agaatcccct tgaccggtat ttccatcttc ttaccgttca
     2581 aggtgcgagg caccaccgac acggcgagga tctcgtccgg cacgtgacgc ggcgacaaat
     2641 cctgccgaat ccgctcacgc agccgcccct tcaaagcatc atccaactga gcccccggcg
     2701 cgagcacgac gaacagccac aacttgcctg cctctgcgcc ctcggctcca tcgcccagcg
     2761 cgctggtgtc caccacgagg ctatccacga actcgggcag gctttccact actcgataga
     2821 attcggcggt gcccatgcgc actccgccgc gattcaaggt ggcgtccgaa cggccataaa
     2881 tcacgaagct gccgcggctg gtttgcttga tccagtcccc gtggcgccag acacccgcgt
     2941 aggagtcgaa gtaactcttg atgcgccgct tgtcgttcgg atcattccag aaactcaccg
     3001 gcatgcaggg catcggctcg gtcaaaacca gctcccccac ttcgtccacg acggggcagc
     3061 ccgtctcgtc gaaggcctcc acggccgccc cgagccctgc acactgaatc tcgcccgcgt
     3121 gcaccggcag aagcgggcac ggcaacacga aggccgtgca cacgtccgta ccgccactca
     3181 atgagcccaa ggccaaatcc cgatgcacgt tctcgtaaac ccaggcgaag ccctcggcgg
     3241 ataacggggc gcccgtactt ccgagcccct tcagactcga gagcttgtgt gcgcgggcgg
     3301 gcacgattcc ggccttttta cacgccaaca agtgaggcgc gctcgagccg aagtaggtga
     3361 tgctctcttc ctccaccaag cggaacagcg cgttcaaatc cgggtaggcc gggctgccgt
     3421 cgtacaacac caccgtagtg ccgagcagca ggccgctgat caagaagttc cacatcatcc
     3481 agccggtggt cgtgaaccaa aaaaatcgat cgtcctgatc caggttcagg tgcaaggcga
     3541 gcgccttgag gtgctccaac aacatgcccc cgtggccgtg cacgatggct ttcggcagcc
     3601 ccgtggtccc cgaggaatac aagatccaaa gcgggtgttc gaaaccgacc ggctcgaact
     3661 cgagcggctc ctcttcggcc agaagctcgt cccaacccag catcccctcg cgtgcctcgc
     3721 ccacgtgcac ggtggccgcg aggctcggca aaccggcccg cacgcgtgcg agctccgcgc
     3781 tgcgatcgaa acgcttgcca ccgtatacgt agctcgagct cgccagcagc accttcgggg
     3841 cgatttgttg aaagcgatcc aacacgctgc tcgcgccgaa ctcgggcgaa caactcgacc
     3901 agatcgcgcc caggctcgcg caagccagga agccaatcac ggcctcggga cgattggtga
     3961 ccaacgccgc gacccgatcg cccacgccaa cgccaagacg acgcaacccc gctcgcgctc
     4021 gagccaccgc gtgcgccagc tcgcttcgac tcaacacctg gcgtggctcg ctctcggagc
     4081 gaaagatcag cgcgtcccgc ccgtcacgtc gagacagggc atgcgccgcg tagttcagcc
     4141 gagccccgtc gaaccattgc acgtgcggca tcgtcccgcg cacgcgctct gacacggtgc
     4201 tctccgagcg cacgccgaag tagtcccaaa tcgatcccca gaagcacggc aaattctggg
     4261 tactgaaggc gtgcaggctc gggtagtcgt cgaagacgta acctcgggta tgccgcagcc
     4321 agccgatgta gtcggacatt cggcacgacc gcactcgatt caaagaaggc gcccagagca
     4381 actcgccttc agccacgacg cgacgactca tgagtgaacg cctccaactc gacctgcgta
     4441 gccctcgtgg gcgccccccg catcggtacc ggctcccgcc gcaaggtcaa cgactcggct
     4501 cggcgcaccg cctgcgtgag ccccaaaacc gagcgcacgc tgcagcgcgc tcttctccgc
     4561 gcgagccccg cgccgccaga gcaagccatc gagcaggtag tgagtcactt gcgggagcgc
     4621 caacagcgga accagccacg cgatccattg cgtcaaaggc ccctccccgg cggtatgacc
     4681 tccgaacagc caaacccgat cgtgatccac ccatcgatcc caagcccatt cctctaccca
     4741 ggccaggacc cacaacgcgc agacgaacgc ggcgacactc tgccccgcca cgcgcagggc
     4801 cgccggcgcg ggcgcctgct cgcgccgctc gcgcgtgtac gcgaacaaaa accagacgta
     4861 cggcaccccg tgaggaatca cgttcgtagc ggtgaacacg aaatccgatt cgttgaccac
     4921 gattccgacg taccaagtga cagcggtaga gagcaccaaa agaaccctcc ccaccgccat
     4981 ggcttgcccg agccacagtc gccgcagctc tcgagcgaag aacgatgcca gagccagcgc
     5041 ccagagcacc gttgaagcgg gcaggatcct ctcgaggtcc aatgctacga aatctccggg
     5101 cgaaagccag gagaagtgct tctcgctcga gcgagcgtgc caaacgatca gcggatagag
     5161 cgttgccgcg taaaccgcgg cctcgtccac gcaacgttcg agtgacccgc ggagccccgc
     5221 ccgcgcgcgg tacaacgcca cccaacccac ctgctgacgc acgaagtgaa aaaccgcgag
     5281 ataggccact actcgccaga acatccccgc tcgatacgca cacacgccgc ctatgtacgc
     5341 cagcagcggg acgagcgcgt agcgcgcagg atgacgacgc agctccgaaa cgtcgaagta
     5401 cgtccggaac cacgtggcgt gcacgtgcgc gacatcgatc gccagcacga gcgcgagcca
     5461 ggcccagtcc gaaacctccg tcacacccag cgccgacgcg gccgcggaga gcagcagcgc
     5521 caccagcgcg ctgccggtga ataccgccaa atcgacgcga gcgccgaaca accacgggcc
     5581 aaagcgagag cctgagccat tcacgcgcgt cgcggccgtc acgcgccgat gatgcctgcg
     5641 gaaacccgcg cgatgcaata tggagttggt ggctcgcgcc gagcgcttgc tgggcgcgcc
     5701 cgcggtctac gggcgcgaaa cccgtcacgt gcctccccca cgcggcgtgg cttgccgcgt
     5761 gggctcagcg cgccgcgcgg gcgccgcgtg gtcgaatccg tcgcgacgct ctcggctcac
     5821 gtctcagcaa cgccgtcgtc acgaagcgct gctcgcacga ggcgctcgcc acaatattct
     5881 tgcgctgccc c
//
LOCUS       DASTKU010000163         6719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_252258, whole genome shotgun sequence.
ACCESSION   DASTKU010000163 DASTKU010000000
VERSION     DASTKU010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252258"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(10..214)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(430..1080)
                     /locus_tag="VFQ61_05840"
     CDS             complement(430..1080)
                     /locus_tag="VFQ61_05840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9954001.1"
                     /translation="MKTPPPLDTISERTLGHYETHATSFWAGTRDHDVSQNYQALLNA
                     LDGERRRLRILDFGCGPGRDLKFFQELGHEAVGLDGCAAFVEMARTHSGCDVLHQSFF
                     ALELPPLRFDGVFANASLFHVPRALLPRVLQQLKETLVPGGVLFCSNPRSFDDDREGW
                     QGERYGTYLTRESWAELIRNAGFSLERQFLRPEGKPCTEQPWLAMVWRNPPEGNAP"
     gene            complement(1107..2354)
                     /locus_tag="VFQ61_05845"
     CDS             complement(1107..2354)
                     /locus_tag="VFQ61_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954002.1"
                     /translation="MAELGRGRRPEAGGSLKGSVKSLVCENRSPCRVLEVQDAGKAPG
                     GLSLEVIKVSLAAKSRPELDPARLGEPCEPIEYWLITRERTHAQSTELLLELCNDGYG
                     AAAVGEDTIEITDNRFSHSQSGGSGWRWATTRVLQLVPRRYVELRNVGYRSADANRNQ
                     LLWNWDAFSGMEFHYAPECDAKGAPPESPVDGDRGQTWTEIPVVDAEAEFMNEGWRTT
                     ALGACAAQVMTEGPALAEGTSGFVVHGKHDGSEDSQFRVVGLNDGSFVLEIHDDQLVT
                     RAKRPLFEDHVEIWSSDAAALNYSYNCEEKRTERPVQWGVRLTDGGVFSGSGAPPIEE
                     VQVELGERTAHVVHLKVRVHRPRSLITFVYSDSDDGEKQKSLIATSDLVFGRSVSLGQ
                     TLKIDPKDATCRVRSGRLEPVLK"
     gene            3035..4144
                     /locus_tag="VFQ61_05850"
     CDS             3035..4144
                     /locus_tag="VFQ61_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954003.1"
                     /translation="MAGSAGMGGSYAAAGGSRASGGVGPSGGSAATNMQGSGSILGAA
                     GAGSTSTAAGTSGVGGQTWSGCPPETKTQTKAGCRDRIEEYPAGRAPSSIAVDSEGMI
                     WFEDMEKQQIVRVDRQGNFISAIQAETAPWPRELLRGDGDVLIWFTDSLDHGLHRFTK
                     QQAHESVFVGEKPRGLAEGPDDDILVVDSGKSIYRVKKSFEGISVLDAAASNSVALGA
                     DKNFWFPSHENFAIGKLTAEGNPTYYSVSNGFPDYICAGPDGALWFTDSSEKKIGRID
                     TKGGRAIFGLPDAGQDAYQITAGSDSAIWFTDRRASKIGRVVLSGGDPVFAYYPTPSP
                     NSSPTGITLDEDGTIWFTENRTGKIGHLYPDPTDL"
     gene            4169..5716
                     /locus_tag="VFQ61_05855"
     CDS             4169..5716
                     /locus_tag="VFQ61_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET9954004.1"
                     /translation="MHGQKQANLRCCFGALALLSGILGFGCNVFGLESSSNPESVCGS
                     TYDLQDVELYDGSAGVSRSFVNRQRRAVGLLRWKNDLEKRYRENPGNVNGKGWCTGTL
                     ISNNMFLTAAHCLDGKGKNVPKEKGRVAIEPTALAREFVVEFRYEISASGAPITAQTL
                     EVQSLEEYKLGELDYAILRLSDDASISNGVTGIAPRNAAAGNRIAILQHPNGSPMRVG
                     AGRVARVDEHKISYDTIDTEGGSSGAGILDVVTGKLVGIHTNGGCTEHGDSANYGVPI
                     EDLLRVSPILRAKIDYSKDFWVGDWNNDARDDLAVFHDDRLYADTNGDGVQDGYVTPE
                     RDAEEYFTGRWRVGAGTSLGWRRGGCLYLDVAPAQALCYGAATDPFDLLIADWDGDGR
                     SDLGIQHGRCIDFDTDLDGRIDQSGYCFGNGLAEDQYLAGKWDGGTRDSIAVRRGAEL
                     FADMDRDGVADASRYFGNGGDDSQYLAGDWDGSGRTELAVRRQLNLEGSEPGSMIRSY
                     QPFWKAP"
     gene            5903..6706
                     /locus_tag="VFQ61_05860"
     CDS             5903..6706
                     /locus_tag="VFQ61_05860"
                     /inference="COORDINATES: protein motif:HMM:NF025631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4360 domain-containing protein"
                     /protein_id="HET9954005.1"
                     /translation="MNSDTIRLFRSFRTSLLLSVAGAALACACGAADAPETSETEQNA
                     VRAAPSGSAESLAISFTDGSDQSAPPPSTLTNTTEPSTGTVVGTGGTSSGALSAELLS
                     ANGSGCPGGPATLVPGATPEAFTLKFPSYSTSLGGGAGVAERRRNCQMLLNVTPPMGY
                     TFTVASASLSGSANLSSGVTARAMVSYHFTGEPETGLSTYQIPSAGGKWSTTGVFAAE
                     TLAYAPCDSQRFLAVNTSIALSGQDDPAVKSTAALDPGTTVQLQLRKCQ"
BASE COUNT         1305 a         2022 c         2098 g         1294 t
ORIGIN      
        1 ctcgcagaaa acgcgccgtc ccgttcgggt aggtctcctg gctcatgggg ccgagcagat
       61 tcagctcggt gtatatggag cttccgacct tccccgaatt tccttcgagt ggtctttgga
      121 agttcactcc caattacagt ggcgggaccg cgccggattc acaccggctt ccctgttctg
      181 cctgttgact gcagcttgtt agctgaagcc tcttggcatg tgccttgtac ggcacccgaa
      241 cgagtatctg acctttggac gtggcacacg taccctgacg taggggatgc tgtcaagcga
      301 atcgacccat ccagttggac gctcgtacat ctgacgcaat ccaacgctcg agcgcgcttg
      361 gcatctggcc ctctgacctc tcgtcaagcc gccagtttca tttgggagcc gggtgagagt
      421 ctgcgggatt tagggggcgt tgccttctgg ggggttcctc cacaccatcg cgagccatgg
      481 ttgctcggtg caaggcttgc cttcgggcct caagaattgc cgttcgagtg agaatccggc
      541 gtttcgaatc agctccgccc agctctcgcg ggtcaaatag gtaccatacc gttctccttg
      601 ccacccttcg cgatcgtcgt cgaaggaacg cgggttggag cagaacagca cgccacccgg
      661 cacgagcgtc tccttcaact gctggagaac ccgtggcaac aacgcgcgcg gaacatggaa
      721 cagagaggcg ttggcgaaga cgccgtcgaa gcgcagcggg ggaagctcca gcgcgaagaa
      781 gctctgatgc agtacgtcgc agccagaatg agtgcgcgcc atctccacga acgccgcaca
      841 accgtccaac ccaaccgctt cgtgaccgag ctcttggaag aacttcaggt cacgacccgg
      901 tccacatcca aagtccaaga tccgaagccg cctccgttcg ccatcgagtg cgttcaacag
      961 agcctggtag ttttggctaa catcgtgatc acgggtgccg gcccagaagc tcgtggcgtg
     1021 agtttcgtag tgacccaacg tgcgttcgga tatggtgtcg agcggaggcg gagttttcat
     1081 tggcttggtg agcccttcga gtggaattat ttcagcacgg gttcgaggcg gccggaacgc
     1141 acccggcacg tcgcatcctt cggatcgatc ttcagcgtct gtccgagtga tacactgcga
     1201 ccgaatacga ggtcgctcgt ggcgatcaac gacttctgtt tctcgccatc gtcactatcg
     1261 ctatatacga aggtgatcaa cgagcgaggc cgatgcactc gaaccttcag gtgaacgacg
     1321 tgagccgtac gctctcccag ttcgacttgg acttcttcta tcggtggcgc ccccgagccg
     1381 ctgaagacgc caccatccgt aagccgaacg ccccactgca cggggcgctc cgtgcgcttc
     1441 tcttcgcaat tgtaactgta gttgagagcg gccgcgtcac tcgaccaaat ctccacatgg
     1501 tcttcgaaga gagggcgttt cgcacgggtt accagctgat cgtcgtgaat ctctagcacg
     1561 aagctcccat cattcagacc gaccacacgg aactggctat cttcggaacc atcgtgtttt
     1621 ccgtgcacga cgaagcccga ggtgccttcg gccagggccg ggccctccgt catcacttgg
     1681 gccgcacagg ccccgagcgc cgtcgtgcgc cagccctcgt tcatgaactc cgcctctgca
     1741 tcgacgacgg gtatttcggt ccaagtctgg cctcgatctc catccaccgg agactctggt
     1801 ggcgcgcctt tcgcgtcaca ctccggcgcg tagtggaact ccatgccact gaatgcgtcc
     1861 cagttccaaa gcagctgatt ccgattggcg tccgccgacc ggtagccgac gtttcgaagc
     1921 tccacgtagc ggcgaggcac gagctgcagc acgcgggtgg ttgcccaacg ccagcccgag
     1981 ccgcccgact gactatggct gaaccggtta tccgttatct ctatcgtatc ctcgcccacg
     2041 gccgcggccc catacccgtc gttacagagc tcgagtagaa gctccgtgga ctgcgcgtgg
     2101 gtccgctccc gcgtgatgag ccagtactcg atgggttcac acggctcgcc gagacgcgcg
     2161 ggatccagct ctggtctaga cttggctgcg agcgagacct tgatgacctc taggctcagc
     2221 ccgccgggcg ctttgcctgc atcctgaact tccaggacgc ggcagggact tcggttctcg
     2281 cacactaggg acttgacgct gcccttcaga ctgccccctg cttcgggtcg gcggccgcgc
     2341 ccgagctccg ccatacggac gggccatggg tccgattgcg catcgggatg gagcgccgct
     2401 ctaggtagtg tcttcgtgct ctggcccgca ctgccagcgg acgcagcaac gctggcaggg
     2461 atcccactac cgcttggcac cggagtgctg gtcgaggaag cggcagagcg tggtgctgcg
     2521 gtcggcgcga cttgcttctg agagctgacg cccgcggctg cccgctgcgc ggctttgctc
     2581 cggcaagcgg ccacgccaca cagaacgaac agcacccagc atactgattt tggcctgcgc
     2641 agcattgcga caatttaggc gaatctggct cgtgcgggta gctcgcgcac gagctttcaa
     2701 cccgttcttc tccaaaccaa cacgggaggg gatggaaggt gaagcgatgc aaaaccccgt
     2761 tcgaatggtg atcgctgtgg cagcgctcaa ttgtgcatgc agcgcacaag cagaaaaagc
     2821 gaaccggaac tcagacacga taaactcctc cggttccacc aactcactcg gaaacccact
     2881 gccgcctgcc acgatgaggc ctgggaccgg ggctggtggc tggagctcgg gtggggcgcg
     2941 cggcggaacc acatcctctg gcgcaagcac atcctacggc ggaagcacgt ctctgagcgg
     3001 ctctggaggc acggggggta ctacaggatc cgcaatggcg gggagcgctg gaatgggggg
     3061 cagctacgcc gccgccggcg gctcgcgtgc gagtggagga gtgggcccga gtgggggctc
     3121 cgcggctacg aatatgcagg gctcgggatc gatcctcggt gcggcgggcg cgggatccac
     3181 gtccacagcg gcaggcacga gtggagtagg cgggcagacc tggagcggtt gtccgccaga
     3241 gacgaagacg cagaccaaag cgggctgccg tgatcggatc gaggagtatc ccgccggcag
     3301 ggcgccgtcg agtatcgcgg tcgattcgga ggggatgatt tggttcgaag acatggagaa
     3361 gcaacagatc gtgcgtgtcg atcggcaggg aaacttcata tctgcgattc aagccgagac
     3421 cgcaccttgg ccgagggaac tgctccgggg tgacggcgac gtgctgattt ggttcacgga
     3481 ctccctggac cacggcctgc ataggtttac caagcagcag gcccacgaat ccgtattcgt
     3541 cggcgaaaag ccgcgcgggc ttgccgaggg gccggacgac gatatcctcg tcgtggactc
     3601 gggaaaatcc atctatcgag tgaagaagtc gttcgagggc atttcggtcc tggatgccgc
     3661 cgcgtcgaac tccgtcgcat tgggagccga taagaacttc tggttcccat cccacgaaaa
     3721 cttcgcgatt ggcaaactca ccgctgaggg caatcccacc tactattcgg tctcgaacgg
     3781 cttccccgat tacatctgcg ccgggccgga tggagcgttg tggtttaccg atagttcgga
     3841 gaagaagatc ggtcgtatcg acaccaaggg gggacgcgcc atcttcggct tgccggacgc
     3901 tggccaagat gcctaccaga tcacggccgg aagcgacagc gcgatttggt tcacggaccg
     3961 cagagcgagc aagattggtc gcgtcgtgtt gagcggcgga gatccggtgt ttgcgtacta
     4021 ccccacgccg tcacccaact cgagccccac cgggatcacc ttggatgaag acggcacgat
     4081 ctggttcacc gagaaccgca ccggcaagat agggcaccta taccctgacc caaccgatct
     4141 ctgatcgaca ccgaggacag gtgaggacat gcacggtcaa aagcaagcga atctgcggtg
     4201 ttgtttcgga gccctcgctc ttctctccgg cattctagga tttggctgta acgtgttcgg
     4261 attggagtct tcgtccaatc cggagtcggt gtgcggctcc acctacgatc tacaagacgt
     4321 cgaactctac gatggcagcg ccggagtctc tcgatctttc gtcaaccgtc agcggcgcgc
     4381 ggtcggtctg ctgcgctgga agaacgactt ggagaagagg tatcgagaaa accccggcaa
     4441 cgtgaacggt aagggttggt gcacgggcac gctcatctcc aacaatatgt tccttaccgc
     4501 cgcacactgc ttggacggta aggggaagaa cgttccgaaa gagaagggcc gagtcgcgat
     4561 tgaacccacc gccctggctc gggagttcgt ggtcgaattc cgttacgaaa tttcggcttc
     4621 tggcgccccc atcaccgctc agaccctgga ggtgcagagc ctagaggagt acaaactcgg
     4681 cgaactcgac tacgccatcc tcaggctttc cgacgacgca tcgatcagca atggcgtgac
     4741 gggcatcgcg ccgcgaaacg cggccgcggg gaaccgcatc gcgattctac agcatccgaa
     4801 cggcagcccc atgagggtgg gcgccgggcg tgtcgcccgc gtcgacgagc acaagatctc
     4861 gtatgacacg atcgacacgg aaggcggctc ctccggcgcg gggattctcg acgtcgtcac
     4921 ggggaagcta gtgggtattc ataccaacgg tggttgcacg gagcacgggg atagcgcgaa
     4981 ttacggcgtg ccgatcgaag atctattgcg cgtctcaccc atcttgcgtg cgaagatcga
     5041 ctactcgaag gacttttggg tcggcgactg gaacaacgat gcgcgtgacg atctcgccgt
     5101 gttccacgac gaccgtctat atgccgatac gaatggcgac ggagttcagg acggctatgt
     5161 tacgcccgaa cgcgacgcgg aagagtattt cacggggcgg tggcgagttg gcgcgggaac
     5221 ttcgttgggt tggcggcggg gcgggtgctt gtatctcgac gtcgctccag ctcaggcgct
     5281 ttgctacggt gcggctaccg atcccttcga cctgctgatc gccgattggg atggcgacgg
     5341 gcggagtgac ttggggatcc agcatggccg ttgcatcgac ttcgataccg acttggacgg
     5401 tcggatcgat caatcgggat actgttttgg gaacgggctc gcggaagacc agtatctcgc
     5461 gggcaagtgg gacggcggta cccgcgatag cattgcggtc cggcgtggcg cggagctctt
     5521 tgccgatatg gaccgggacg gggttgccga cgctagtcga tactttggga acggcggaga
     5581 cgatagccag tatctagcgg gcgactggga cggctccggc cgtaccgagc tcgcggtgcg
     5641 ccgccagctg aacctggagg gaagtgagcc aggttcgatg atacgcagct atcagccgtt
     5701 ttggaaggct ccctaataca ctcgatatca gagggtgggg tcgggccgaa tttgcgcgcg
     5761 aatcacccga aagtggccag gccaacacgg caatagagca ttcggacgcg ctccgagaga
     5821 tcgagtctct gcgcggcgct aacgcgcaat tcttgagttc attgtgttca acatcaatct
     5881 caatccatca agaggtatca tcatgaatag cgacacgatt cgattgttcc gtagttttcg
     5941 tactagcctt ctgctctcgg ttgccggcgc cgcgctcgcc tgtgcctgcg gcgcggcaga
     6001 tgcgccggag acgagtgaaa ccgagcaaaa cgcagttcgt gccgcaccct cgggttctgc
     6061 ggagtcgctg gcgatcagct tcaccgatgg atccgatcag agcgctccgc ctccgagcac
     6121 gctcaccaat accacggagc caagcacggg gactgtggta ggcacgggcg ggacgagctc
     6181 tggagctctg tccgccgagt tgctctcggc caacggctcg ggttgcccgg gtggccccgc
     6241 aaccttggtt ccaggtgcta cgcccgaggc gttcacgctg aagttcccga gttactcgac
     6301 gtctctcggg ggaggagccg gggtcgcaga gaggcgccga aattgccaga tgctactcaa
     6361 cgtgacccca cccatggggt atacgttcac ggtggcaagt gcctctctga gcgggagcgc
     6421 aaacctctcg agcggggtca ccgcacgagc catggtttcc taccacttca cgggtgagcc
     6481 ggagaccggt ttgtccacct atcaaattcc gagcgccggc ggtaagtgga gtacgacggg
     6541 agtcttcgcg gcggagactc tggcttatgc tccttgtgac tctcaacgct ttctcgccgt
     6601 caacaccagc atcgcgctct cgggtcaaga cgaccctgcg gtcaagagca ccgcggcgtt
     6661 ggatcctggc acgacggttc agctccagct gcgaaagtgc caatagcgga tacactgga
//
LOCUS       DASTKU010000164        12548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_252439, whole genome shotgun sequence.
ACCESSION   DASTKU010000164 DASTKU010000000
VERSION     DASTKU010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12548
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252439"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VFQ61_05865"
     CDS             complement(<1..804)
                     /locus_tag="VFQ61_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HET9954006.1"
                     /translation="MTTAPVGANAPRHTAKAPARARKPMSWEWAPRANTYILVGLTVL
                     GLILRIRRPDLPENFTFDEALFVKNAHHYLIGSPDDNDHPPLGKLLMALGQIAFDYNS
                     LGWRFTSVCFGVLTVYMAYLLGKNLFASERAGYFSAALIAADGFFIAYSRAGLLDGVL
                     CCLILWTVLAAVVARNFWDVLITGLLLGLATSVKWSGAFAVFPAALALVLLQRLPWYW
                     VPALGISLIVHVLIWMIGLHITGKPSDLPALWGVMKGLFHHHLDLGSRDN"
     gene            complement(801..1562)
                     /locus_tag="VFQ61_05870"
     CDS             complement(801..1562)
                     /locus_tag="VFQ61_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954007.1"
                     /translation="MLPERNPSKVAFTETMVETNIGTSAEPSGSATPPRAQTENSSAE
                     VDSSGANAKGAQPGEASTASGATASGAAGAAVSAGNDALQAPPDRAVHARLLAALFGI
                     FFVLWAFYAWRGYGSRHAQLGESWYVGGVHLIELTLVKEDAVNLACASDTVHKGLHCA
                     FRGDQRTPYPDLKDATMLKPYYTVKGELLFGAGLWSSPAFQRALPRTRFTAACNYHVV
                     GIMKAASTRWAEKGRFDPLKQSATFGYFTDCVLPE"
     gene            complement(1728..2798)
                     /gene="dapE"
                     /locus_tag="VFQ61_05875"
     CDS             complement(1728..2798)
                     /gene="dapE"
                     /locus_tag="VFQ61_05875"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HET9954008.1"
                     /translation="MSLTETLLWLCEIPSPIGEERALADALWARFASESFDEPARRYG
                     DSLVFPVCRGRGGPHVVLAGHLDVVRTQHDGPPRIEGDKLYAPGAADMKSGLALMVDM
                     LQRPPLGGAKRGLDLSVVFYAREEGPYAENELGLVLEQDQELRHASIAVMLEPSDNKL
                     QLGCGGSLHARVTFHGRTAHSARPWQGENAIHKAAGLLARLSSLPVREQDCEGLRWRD
                     VISATMAQGGRARNIIPDRFELNLNHRFGPLVDLEQAERNVLDLVAGDAEVEFVDRSP
                     SALPYRAHPLVQALAESGVAAVEPKQAWTDVARFAAMGVPAVNFGPGVQAQAHQRNEW
                     THLPALETGREILSRWLRRITS"
     gene            complement(2840..3664)
                     /locus_tag="VFQ61_05880"
     CDS             complement(2840..3664)
                     /locus_tag="VFQ61_05880"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET9954009.1"
                     /translation="MHESELKSRIESAFEDRKLLENAETKAAVEEVIQLLDTGKLRVA
                     SRGEDGKWVTHAWIKQAVLLYFGITEMRTMSAPPFEFYDKIPLKKNLAEFGVRVVPPG
                     TVRYGAFIEKGAIVMPGYVNIGAYVGSGTMVDTWATVGSCAQIGRDCHLSGGVGIGGV
                     LEPPGAQPVIVEDGCFIGSRAIVVEGMLIEKEAVIGASVALTASTPIIDVTGSSPVEY
                     RGRVPARSVVIPGMRTKKFPAGEFQVACALIIGQRKESTDKKTSLNSALRDFNVSV"
     gene            complement(3728..4507)
                     /locus_tag="VFQ61_05885"
     CDS             complement(3728..4507)
                     /locus_tag="VFQ61_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954010.1"
                     /translation="MSQLLHSLRVAPKAVSQVLPDTLEACASLRTATDTWMNAVNDAR
                     VQPARRALSGFCLPLIAELEAKLRESIAKPMNAKSRLALEEVVAKCAAELNSARELLE
                     LVEDALFASRVRLDPRELVRETFTARVHAPSQGGVLVSAMLSSYDPGSETEINPQVAM
                     ALVAFGVELVGAASTRETPHVLVSSNPQGVPLVRITRKAHATGEPLVLQSRGVREPTL
                     PCVKIAAALADGHVEWEPGGSEFVLRLPAAEPWIASRTHSA"
     gene            complement(5115..5531)
                     /gene="ndk"
                     /locus_tag="VFQ61_05890"
     CDS             complement(5115..5531)
                     /gene="ndk"
                     /locus_tag="VFQ61_05890"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET9954011.1"
                     /translation="MSQRTLCIIKPDAFEKGHAGAIIERILKERFRVLGLRQLHLSRK
                     QAEGFYAVHRGRPFFDELCAFMSRGPVVVAALEREEAVQHWRNVIGATDPAKAATGTL
                     RQLFGASLGENAVHGSDSEENGRIECAYFFAGMDLL"
     gene            complement(5647..6543)
                     /gene="sucD"
                     /locus_tag="VFQ61_05895"
     CDS             complement(5647..6543)
                     /gene="sucD"
                     /locus_tag="VFQ61_05895"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET9954012.1"
                     /translation="MAILVDKNTRVVIQGITGSAGSFHAKQCIAYGTQIVAGCTPGRG
                     GEKFAATDAAGKAVEVPVFDTVAEAVQRTGADTACIFVPALGAADSILEAANSGCKLV
                     VCITEGIPVTDMIRVRRALEGKPVRLIGPNCPGIITPGACKIGIMPGHIHRTGRIGVV
                     SRSGTLTYEAVGQLSALGLGQSTCIGIGGDPVGGMDFVDVLSLFNDDPDTDAVMMMGE
                     IGGNAEERAAEFIKKSMKKPVAAFIAGTTAPPGKRMGHAGAIISGGKGTAADKIRALE
                     AASVRMAPSPAEMGSTLKSLLS"
     gene            complement(6559..7758)
                     /gene="sucC"
                     /locus_tag="VFQ61_05900"
     CDS             complement(6559..7758)
                     /gene="sucC"
                     /locus_tag="VFQ61_05900"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET9954013.1"
                     /translation="MKIHEYQGKQIFARYGIPIPKGYACFTAKEVEEAAAKLRTETGS
                     DVVVVKAQIHAGGRGKGGGVKVVKGGPTEAAAVAEKLLGMQLVTHQTGPEGQRVRRLY
                     VEQGLDIARELYLGLVVDRERRRIAVMASTEGGVEIEKVAEESPEKILTEHVDPVTGL
                     SPFQARRLGFGLQITAGSPVPKETFAEFTRVLGKLVELFEKEDCSLCEINPLIVTRQG
                     KIVALDSKINFDDNAADRHKDWKELMDRDEEDPVELEAKEAGLSYVSLDGDIGCLVNG
                     AGLAMATMDIIKHHGGSPANFLDVGGGATKDQVTHAFKMILSSPKVKGIFVNIFGGIM
                     KCDVIAEGVVAATRELGLKVPLVVRLEGTNVEQGKKILNESGLRIQAADGMADGAKKI
                     VSAIQAA"
     gene            complement(7897..8835)
                     /gene="mdh"
                     /locus_tag="VFQ61_05905"
     CDS             complement(7897..8835)
                     /gene="mdh"
                     /locus_tag="VFQ61_05905"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET9954014.1"
                     /translation="MARKKIGLIGAGNIGGELANLAVNLKLGDVVLFDIPAKENFAKG
                     KALDLEQATALSGADSKVTGTSRWEDLEGADVLIITAGIPRKPGQSRDDLVATNLPII
                     RNVADSAKKYCPDAFVIVISNPLDAMVYEYRRRVGAPREKVLGMAGVLDSARFSLFLA
                     RETNASIKDVRAMVLGGHGDDMVPALSYCTINGVPVRQLLSKETLAKIVDRTRKGGGE
                     IVNLMGTSAYYAPASSAIAMAEAYLLDQKRLLPCAAYLDGEYGYKDMYMGVPVIVGAG
                     GGEKIVEIELDAEEKAMLKKSAESVQKVVDIVKASS"
     gene            complement(8845..10233)
                     /gene="icd"
                     /locus_tag="VFQ61_05910"
     CDS             complement(8845..10233)
                     /gene="icd"
                     /locus_tag="VFQ61_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HET9954015.1"
                     /translation="MAVFEGPKAGDAIQMVSGKLNVPDQPIVPFIEGDGTGPDIWRAS
                     QAVLDAAVAKAYGGKRKIAWYEVYAGEKSKKLFDSWLPNETVEAYRKYLVGIKGPLTT
                     PVGKGIRSLNVALRQQLDLYVCLRPVRWFTNVPSPVKRPGDVDMVIFRENTEDIYAGV
                     EWAAGTPEVKKVLEFLQKEMGVTKIRFPESSGIGIKPISREGTERLVRAAIQYAITNG
                     KKGVTLVHKGNIMKFTEGAFRDFGYELAKREFRSQIVTEREAWILGNAEEKPSLSAEE
                     NAKLVEPGFDMAPKETQAELVAEVQAALALAPTHGNGKWKKMLRIKDSIADITLQQVL
                     TRPKDFDVIATMNLNGDYLSDALAAQVGGIGIAPGGNINYDTGHAVFEATHGTAPKYA
                     NLDRVNPGSVTLSGEMMLRHLGWNEAADLVIKGMDGAIGAKTVTYDFARLMEGAKEVK
                     CSEFGRAIIEHM"
     gene            10543..11067
                     /locus_tag="VFQ61_05915"
     CDS             10543..11067
                     /locus_tag="VFQ61_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954016.1"
                     /translation="MQSAEDVEAAGVQCSPTAASLEGRYHGLGSGVSCEAELQLRMKQ
                     VSRALGVDCPYCHAGSDYARDTERKRIANWMAIELAPKLREKGSGRPARCSSCHLVDG
                     VGRAKLLGNPRAEPVALEWMNTHMVADFVTQNGALLRCKTCHRAQFGAPGFARRLILT
                     SNPIAGTSSAPLPE"
     gene            11142..11573
                     /locus_tag="VFQ61_05920"
     CDS             11142..11573
                     /locus_tag="VFQ61_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9954017.1"
                     /translation="MTTSSRSMVSPRPPRVLIVDDDPDVVSALVRLLRAAEPDWETRT
                     ARDGQEALACFERESFDVVMTDLQMPSMDGFQLLRRLARSHPETIRIVHSSHGAMLGT
                     ELVRYLAHNVVAKPSPSGEVLSLLRWAVRAAGSVERNASRA"
     gene            11963..>12548
                     /locus_tag="VFQ61_05925"
     CDS             11963..>12548
                     /locus_tag="VFQ61_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954018.1"
                     /translation="MSYGFLRGLRGSTSRVWEPRSFIQHAWFPLLCSVWLTACGSDSN
                     GTAAGGSPSGGAGNAGSGGSPSGGSSGSGGTSNGVGGGSAGMSGSTSPTNGGSSTSGG
                     NAAGGDSSTTGGTAATTGGSANGGKGGGVSTGGASAGGAGSGGKGGASSAGSGGATST
                     SGGAGGASSGCLEFAAPTKAGDIESTALDGPSGIA"
BASE COUNT         2192 a         3958 c         3972 g         2426 t
ORIGIN      
        1 gttgtcgcgt gagccgagat cgaggtgatg atggaacaaa cccttcatca caccccagag
       61 tgcgggcagg tcgctcggct tacccgtgat gtgcagcccg atcatccaaa tcaggacatg
      121 cacgatcagc gagatgccga gtgcggggac ccaataccag ggtaaccttt gtagtaagac
      181 cagcgcgagc gcggcaggaa agaccgcgaa cgccccgctc catttgacgg aagtggcgag
      241 ccccaacagc aagccggtga tgagcacgtc ccagaagttg cgggccacca ccgccgcgag
      301 cacggtccac aagatcaggc agcagagcac tccatccaac aatccggcgc gggagtatgc
      361 gatgaagaag ccatccgcgg cgatcagggc cgccgagaag taaccggcgc gctcgctcgc
      421 aaatagattt ttgccgagta gatacgccat atagacagtc agcacgccga agcagacgct
      481 ggtgaatcgc caacccagcg agttgtagtc gaaggcgatc tggccgagcg ccatcaacaa
      541 cttcccgagt gggggatggt cattatcgtc gggtgagccg atcaggtagt ggtgcgcgtt
      601 cttgacgaag agtgcttcgt cgaacgtgaa gttctccggt aaatccgggc gtcgaatgcg
      661 caggattagc cccaacaccg tcagcccgac caggatgtac gtgttggcgc gcggcgccca
      721 ctcccaagac atgggtttcc gagcgcgggc gggagctttg gccgtgtgcc gtggggcgtt
      781 cgcgcctacc ggtgcagtcg tcattcgggc aacacacagt cggtgaagta gccgaaggtc
      841 gcgctttgct tgagcggatc gaagcgtccc ttctcggccc agcgcgtgga ggcagctttc
      901 atgatgccga ccacgtgata gttgcatgcg gccgtgaaac gcgtgcgggg cagggcgcgc
      961 tgaaacgccg gtgaactcca caatcccgcc ccgaacaaga gctcaccttt cacggtgtaa
     1021 tagggcttca gcatggtcgc gtccttgagg tcgggataag gcgttcgttg atcgccccgg
     1081 aacgcgcagt gcaggccctt gtgcacggta tcggaggcgc aggcgagatt gaccgcgtcc
     1141 tctttcacga gcgtgagctc gatgaggtgc acgccaccta cgtaccaact ttcgccgagc
     1201 tgggcgtgcc gcgagccgta gccgcgccac gcatagaacg cccacaacac gaagaagatg
     1261 ccgaatagcg ccgcgagcaa tctcgcatgc accgcgcggt ctggcggcgc ttgcaacgca
     1321 tcatttcccg cggacaccgc ggccccggcg gcacccgagg ccgtagctcc cgacgctgtg
     1381 gatgcctctc ccggttgcgc gcctttcgca ttcgcgccgg agctatcaac ctccgctgac
     1441 gaattttcgg tctgtgctcg aggaggcgtc gcgctgccag agggttcggc actcgtgccg
     1501 atgttggtct cgaccatggt ttccgtgaag gccactttgc tcgggttgcg ttccggtagc
     1561 aacgctcgtg ccccatcgag ggctcgctcg aggctgccat tgttctatgg agtctcgggg
     1621 attttatcga gccaaagctc gatcgcttcc ggtcgggagt gtgtgccgcc tgtgtgccag
     1681 atgtgccaac ttttgcgtcg agcgcctccg tcggaggctt cgtagtctca gctggtaatc
     1741 cgccgtaacc aacggctcaa gatttctctg ccggtctcga gcgccggcaa gtgggtccac
     1801 tcgttgcgtt ggtgagcctg tgcttgcacg ccgggcccga aattgaccgc cggcacgccc
     1861 atcgccgcga accgtgcgac atccgtccag gcttgcttcg gctcgaccgc cgcgacgcca
     1921 ctttcagcga gagcctgcac caatggatga gcgcgataag gaagtgcaga aggtgagcga
     1981 tcgacgaact cgacctccgc gtcgcccgct accaaatcga gcacgttgcg ctccgcctgc
     2041 tcgagatcca cgagcggccc gaagcgatga ttgaggttca gttcgaaacg atcgggaatg
     2101 atatttcgag cacggccgcc ctgagccatc gtcgcgctga ttacgtcgcg ccaacgcagg
     2161 ccttcgcagt cttgttcccg caccggcagg ctcgagaggc gcgccaacag gcctgctgct
     2221 ttgtggatgg cgttttctcc ctgccacggt cgcgcgctgt gggcggtgcg gccgtggaac
     2281 gtgacccgag cgtggagcga accgccgcag ccaagttgaa gcttgttgtc gctcggctcc
     2341 agcatcaccg cgatggacgc gtggcggagc tcttgatcct gctcgagcac caggccgagc
     2401 tcgttttccg catagggccc ctcttcacgt gcgtagaaca ccacgctcaa atccaggccc
     2461 cgcttcgctc cgccgagcgg cggcctctgc agcatgtcca ccatgagggc caggccgctc
     2521 ttcatatcgg cagcgccagg tgcgtacagc ttatcgcctt cgatacgtgg tggtccgtcg
     2581 tgctgcgttc ggacgacgtc caagtgccca gcgagtacga cgtgcggccc gccgcggcca
     2641 cggcagactg gaaagaccag tgaatcgccg tagcggcgcg cgggctcgtc gaaggactca
     2701 cttgcaaaac gcgcccacag cgcgtccgcc aaggcccgtt cttcgccaat cggggagggg
     2761 atctcacaca gccagagcaa cgtttctgtg aggctcatcc aatttccacc gaggcgtggg
     2821 gagggctaac cgtttcgaat caaacggaca cgttgaagtc gcgaagcgcc gagttgaggc
     2881 tcgtcttctt gtcggtgctc tctttgcgct gaccgatgat cagcgcgcaa gccacctgaa
     2941 actctcccgc agggaacttc ttggtgcgca tgccggggat caccacgctg cgcgccggca
     3001 cgcgacctcg gtattccacc ggtgacgagc ccgtgacgtc gatgatcggg gtcgacgcgg
     3061 tgagcgcaac cgaggccccg atcacggcct ctttttcgat caacatccct tccacgacga
     3121 tggcgcgaga gccgatgaaa cagccgtctt cgacgatcac cggttgggct cccgggggtt
     3181 cgagcacgcc gccaatcccg accccgccgg acaaatggca gtcgcgcccg atctgcgcgc
     3241 aagagccaac cgtggcccaa gtatcgacca tcgtgccgga gccgacgtat gccccgatgt
     3301 tcacgtaacc cggcatcacg atggcgccct tctcgatgaa ggcgccataa cggacggtgc
     3361 cggggggaac cactcgcacg ccgaactcgg ccaggttctt cttcagcggg atcttgtcgt
     3421 aaaactcgaa ggggggcgca ctcatcgtgc gcatctcggt gatgccaaag tacagcagaa
     3481 ccgcttgttt aatccaggcg tgggtgaccc acttgccatc ctcgccgcgg ctcgcgacgc
     3541 gcagcttacc cgtgtcgagc agctggatga cttcctctac cgccgccttg gtctcggcgt
     3601 tctcgagcag cttcctgtct tcgaaggcag attcgattcg gcttttgagc tcggactcgt
     3661 gcatgccccg cattacgaag gctggccgag cggggcaagg cccttcgaac ggaaaacgtg
     3721 ctcgccgtta ggcgctgtgc gtcctcgaag cgatccaggg ctcggccgcg ggcaaccgca
     3781 ggacgaactc ggatccgccc ggctcccact cgacatggcc atccgcgagc gcggccgcga
     3841 ttttgacgca cggcagggtt ggctctcgta cgcctctgct ttgaagaacc aacggctcgc
     3901 cagtggcgtg agccttgcgc gtgatgcgga cgagcggcac tccctgggga ttgctggaga
     3961 ccaggacgtg cggtgtctcg cgcgtggacg ctgcgcctac cagctccact ccgaaagcga
     4021 cgagggccat cgcgacttgc ggattgatct cggtttcgct tcccgggtca tagctcgaca
     4081 acattgcgga aacgagcacg ccaccttggc tgggcgcgtg cacgcgtgca gtgaaggttt
     4141 cgcgtacgag ctcgcgcgga tccaaccgga cccgactcgc gaacaatgca tcctccacga
     4201 gctcgagcag ctcgcgagca gagttcagct cagccgcgca cttggcgacg acctcttcga
     4261 gcgccaagcg gctcttggcg ttcattggtt tggcgatgct ctcgcgcagt ttggcttcga
     4321 gctcggcgat caaaggcaaa cagaaaccgg agagcgcacg acgagcgggc tgcactcgcg
     4381 cgtcgttcac ggcgttcatc caagtatcgg ttgcggtgcg cagagaggcg caagcctcca
     4441 gcgtgtccgg cagaacctgg gagaccgcct tgggggcgac tcgcaacgag tgcaagagct
     4501 gcgacaagtt acgcagtgcc ccaagggtct cgcgcagggt gccgcgcgca ttccctagga
     4561 ggaccgcccg cgatgacagc ggtggacgct ccctttccgt tcccaagacc ctgtacgcta
     4621 gcatggtttt gccagaccgt gtaatgcgcc gtggggtcga acaagccggc cgtcgcacat
     4681 cgcttcggat ccttccctca gtttccctcg gttgctgttt tccctcggtt gctgcttcca
     4741 gcacgctgcc ggctgggtcc gcgttcgctt gaaccgctcg ttcagagtgt tcgtttcagg
     4801 ctgaatgggg taccggccaa ttggcgctca gcttaagtcc aaccgtgttc ggggcctcac
     4861 cagggaaagt agcactccga ggacgcacgg gtgatcaggc ccaacatttt cctgccaggc
     4921 ttatggcttc ctgtaaggct cattggcgca acaaaacaag agacgccagt taacctcagg
     4981 cttgaagtaa gtagggccta cagccgaagt ggcagacctc gagagcgcgc ccagcgcgtc
     5041 agggtgttcg ctaaaacgag gtgctggaag aggcgccgcg tccgcagagg gcgcctctca
     5101 cttcagcgag aaactcagag tagatccatg ccggcgaaga aataggcgca ttcaatgcgt
     5161 ccattttctt ccgagtcaga gccgtgcacc gcattttccc cgaggcttgc tccgaacagc
     5221 tggcgcaggg tgccggttgc ggccttcgct gggtcggtcg ctccaatgac attgcgccaa
     5281 tgttgaaccg cctcttcacg ttccaatgcc gcgacgacga ccggaccacg gctcataaac
     5341 gcgcagagct cgtcgaagaa cggccgtcca cgatgaacgg catagaagcc ctcggcctgt
     5401 ttccggctaa ggtgaagctg ccgcaaaccc aggacgcgaa agcgctcctt gaggatgcgc
     5461 tcgatgatgg cacccgcgtg ccccttctcg aaggcgtcgg gcttgatgat gcaaagggtt
     5521 ctttgagaca tgagcggggc ctcctttagc actcgtacgc ggcttcgggg atctagacga
     5581 tgagagactg gggaatgggc agcaggactg cgccctgcag cccattccaa gatgacggaa
     5641 gctgccctac gagagcaggc tcttcagggt agagcccatt tcggcggggg agggcgccat
     5701 ccgcaccgac gcggcctcga gggcacgaat cttgtctgct gccgtgccct tgcctcccga
     5761 aataatggcg ccggcgtggc ccatgcgttt cccagggggg gccgtggtgc cggcgatgaa
     5821 cgccgcgacc ggcttcttca tggatttctt gatgaactcg gccgcgcgct cctcagcgtt
     5881 gccgccaatt tcgcccatca tcatgacggc atcggtgtcc gggtcgtcgt tgaacagact
     5941 gagaacgtcc acgaagtcca ttccgcccac cgggtcaccg ccgatgccga tgcaggtgga
     6001 ttgaccaagc ccgagcgcgc tcaactggcc tacggcctcg taggtcaggg tgccagaacg
     6061 ggagaccacg ccgattcgcc cagtacggtg aatatgccct ggcatgatgc cgattttgca
     6121 ggcaccgggt gtgatgatac ccgggcagtt cggcccgatc agacgcacgg gtttgccttc
     6181 gagcgcgcgg cgcacccgga tcatgtcggt gaccgggatg ccttcggtga tgcagacgac
     6241 cagcttgcat ccactattcg cggcttcgag gatcgaatcc gcggcgccca gcgccggcac
     6301 gaagatacat gccgtgtcgg ccccggtgcg ttgcacggct tccgccacgg tatcgaacac
     6361 ggggacctcg acggctttgc cagcggcatc cgtcgccgcg aacttctcgc cgccgcgccc
     6421 gggggtgcag cccgctacga tctgcgtccc gtaagcgatg cactgcttcg cgtggaacga
     6481 gcccgccgat ccggtgatgc cctggatcac tactctggtg ttcttatcga ccaaaatcgc
     6541 cattgctctg ggtcctcgtc acgcggcttg aatcgccgaa acaatcttct ttgcgccgtc
     6601 agccatgcca tcggccgcct gaatccgcag cccgctctcg ttcaagatct tcttgccttg
     6661 ctcgacgttg gtgccctcga ggcgcacgac gagtggcacc ttgaggccca gctcgcgcgt
     6721 ggcggcaacc acgccttcgg cgatgacatc gcacttcatg atgccgccga agatgttgac
     6781 gaagatgccc ttcaccttcg gactcgagag gatcatcttg aaggcgtgcg tgacctggtc
     6841 tttggtggct ccgccgccca cgtccaggaa gttcgcgggg ctgccgccgt ggtgcttgat
     6901 gatatccatg gtggccatcg ccaaacccgc gccgttcacc aagcaaccga tgtcgccgtc
     6961 gagcgagacg tagctgagcc cggcttcctt ggcctcgagc tcgaccggat cttcctcgtc
     7021 ccggtccatg agctctttcc aatccttgtg acgatcggcg gcgttgtcgt cgaagttgat
     7081 cttcgagtcg agggctacga tcttgccctg gcgcgtgacg atcagcgggt tgatctcgca
     7141 cagcgagcag tcttccttct cgaacagctc gacgagcttt ccgagtaccc gcgtgaactc
     7201 tgcgaaggtc tccttcggga ccgggctacc cgccgtaatt tgcaggccaa accccaagcg
     7261 tcgtgcctgg aatggcgaca ggccggtaac ggggtccacg tgctccgtca gaatcttctc
     7321 cgggctctcc tcggcgacct tctcgatctc gacgccgcct tcggtggagg ccattaccgc
     7381 gatgcggcgg cgctctcgat ccaccaccaa gcccaggtac agctcccgtg cgatgtcgag
     7441 gccttgctcg acgtacaagc gccgcacccg ctgcccttcg ggcccggttt gatgcgttac
     7501 gagctgcatc ccgagcagct tctcggccac ggcggccgcc tcggtcggcc cgcccttgac
     7561 tactttgacg ccgccaccct tgccgcgccc gcccgcgtgg atttgcgcct tgaccaccac
     7621 cacgtcagag ccggtttcgg tgcgcagctt ggctgccgct tcctcgactt ccttggcggt
     7681 gaagcaggca taacccttgg ggattggaat tccgtagcgg gcgaagattt gtttgccttg
     7741 atactcgtga atcttcatgg agatgcgacc ttatggcggt gacacgggga gcagcgagag
     7801 gcgctttcgt cgagcaccgt gcgcgatggg gggcgggcgg cgtgaagcca agccgcgacg
     7861 cccgacgctg cacggtgctc gatcgaaaga ctggcttcag ctggaagcct tgacgatatc
     7921 gacgactttt tgaacgcttt ccgcgctctt cttgagcatg gctttctctt cggcgtcgag
     7981 ctcgatctca acgatcttct cgccaccgcc ggcgcccacg atcacgggaa cgcccatgta
     8041 catgtccttg tagccgtact caccgtcgag gtacgcggca cacggcagca ggcgcttctg
     8101 atccaacagg tacgcttccg ccatcgcgat cgcgctggag gccggcgcgt agtaggcgct
     8161 ggtgcccatc aggttcacga tctcgccgcc gcccttgcgg gtgcggtcca cgatcttcgc
     8221 gagcgtttct ttgctgagca gctggcgcac cggcacgccg ttgatcgtgc agtagctgag
     8281 cgcgggcacc atgtcgtcgc cgtgcccgcc gagcaccatg gcccgaacgt ccttgatgct
     8341 ggcgttggtc tcgcgcgcca aaaacaagga gaagcgcgcg ctatccagca cgccggccat
     8401 gccgagtacc ttctcgcgcg gcgcgcccac acggcggcga tactcgtaaa ccatcgcgtc
     8461 gagtgggttc gagatcacga tcacgaacgc gtcggggcag tacttcttcg ccgagtccgc
     8521 cacgttgcgg ataatcggga ggttggtggc gaccaaatca tcacgcgact gcccgggctt
     8581 ccggggaatg cccgcggtga tgatgagcac gtccgcccct tccagatcct cccagcggct
     8641 ggtgccggtc accttggaat cagcgccgga aagcgcggtt gcctgctcca gatcgagggc
     8701 cttgcctttg gcaaagttct ctttcgcggg aatgtcgaac aggacgacat cgccgagttt
     8761 cagattcacc gcgagattcg cgagctcgcc gccgatgtta ccagcaccga tcaaaccaat
     8821 cttttttctt gccatggctt cctctcacat gtgctcgatg attgcgcgcc cgaactccga
     8881 gcacttgacc tctttggcgc cctccatcag gcgggcgaag tcgtaggtca cggtcttggc
     8941 gccaatcgcg ccgtccatgc ccttgatgac gagatccgcg gcttcgttcc agcccaagtg
     9001 gcgcagcatc atttcgccgc tgagcgtgac cgaaccgggg ttcacccggt ccaggttcgc
     9061 gtacttcgga gcggtgccgt gggtcgcttc gaacacggcg tggccggtgt cgtagttgat
     9121 gttgccgccg ggggcgatgc cgatgccgcc gacctgcgcg gccagagcgt cgctcaggta
     9181 atcgccgttg aggttcatcg tcgcgatgac gtcgaaatcc ttgggccggg tcagcacctg
     9241 ctgcagggtg atgtctgcga tcgagtcttt gatgcgcaac atcttcttcc acttgccgtt
     9301 gccgtgggtc ggcgcgagcg cgagcgccgc ttgcacctcg gcgacgagct cggcctgagt
     9361 ctccttgggt gccatgtcga agccgggctc cacgagcttg gcgttctctt cggcgctcag
     9421 gctgggcttc tcttcggcgt tgcccagaat ccaggcctcg cgctcggtca cgatttggct
     9481 gcggaactcg cgctttgcga gctcgtatcc gaaatcacgg aaggcgcctt ccgtgaactt
     9541 catgatgttg cccttgtgca ccaaggtgac gcccttcttg ccgttggtga tcgcgtactg
     9601 gatggccgcg cgcaccaggc gctccgtgcc ctcacgggaa atcggcttga tgccgatccc
     9661 cgaggactcg gggaagcgga tcttggtgac gcccatttcc ttctgcagga actcgagcac
     9721 cttcttgacc tcgggcgtac ccgcggccca ctcgacgccg gcgtaaatgt cttccgtgtt
     9781 ctcgcggaag atcaccatgt ccacatcgcc cgggcgtttc acggggctcg gtacgttggt
     9841 gaaccagcgc acggggcgca ggcagacata caaatcgagc tgctggcgca gtgccacgtt
     9901 cagcgagcgg atacccttgc caaccggcgt ggtcagcggg cctttgatac cgaccaggta
     9961 tttgcggtac gcctcgaccg tctcattggg tagccagctg tcgaacagct tcttcgactt
    10021 ctcaccggcg taaacttcgt accatgcaat cttgcgtttg ccgccgtagg ctttggctac
    10081 cgcggcgtcc aaaactgcct gcgaagcgcg ccagatatct gggcccgtgc cgtcgccttc
    10141 gatgaagggc acgatgggtt gatcgggaac gttgagcttg ccagacacca tttgaatggc
    10201 gtcaccggct ttgggacctt cgaacacagc catatcgcac tagcctctcg atgggcgctt
    10261 ccgcgctcga ccgcatgcgg agtagtccag catgttggcc gtggcaaccg ttcctggaag
    10321 attcgggaaa cggaggcagc caagcttccg agttggcctg gccgctcccg ccgtggccag
    10381 aaatgctacg catgtttcgt gtttttgcga aggcttgcgc ggggcgcgat tttcagcctc
    10441 ggtttcgcgt tgggtgcgag cggaggaagc gtcggttgtg ggcctgctcc gcggccgaga
    10501 gagcgctatg acgacgcggg gggccaggcg cggaaatctg ccgtgcagtc agccgaagac
    10561 gtggaggcgg ccggcgtgca gtgctcgcca acggccgcgt cgctggaggg gcggtatcac
    10621 ggcctgggga gcggagtgtc gtgcgaggcc gagctccagt tgcgcatgaa gcaggtctct
    10681 cgcgcgctcg gcgtggactg cccttattgt cacgcgggca gtgattacgc gcgcgatacg
    10741 gaacgaaagc gcatcgcgaa ttggatggcg atcgagctcg cgccgaagct gcgcgaaaag
    10801 ggctcggggc gtccggcgcg ctgcagctcg tgtcacctgg tggatggtgt ggggcgcgcc
    10861 aagctgttgg gcaatccgcg agcggagccg gtcgctctcg aatggatgaa cacccacatg
    10921 gtcgctgact tcgtgacgca gaatggtgca ctgctacgct gcaagacctg tcatcgggct
    10981 cagtttggag cacccgggtt tgcgcgccgt ttgattctca cttcgaaccc gatcgcggga
    11041 acctcctcag cgccgctacc ggagtgatcg cgcgccgcca aaggcgaacg gagcgtacac
    11101 tcgggtacgc catggactgc gctttgcagt ccggatgtgc cgtgaccact tcatcgcgat
    11161 ccatggtctc gccgcgtcca cctcgtgtcc tcatcgtaga tgacgatccg gacgtcgtgt
    11221 ccgcgctcgt tcgcttgttg cgcgctgccg aacccgactg ggagacgcgc actgcgcgcg
    11281 atgggcagga agctttggcg tgtttcgagc gcgagagctt cgacgtggtg atgaccgatc
    11341 tccaaatgcc gagcatggac ggatttcagt tactgcggcg gctcgctcga tcccacccgg
    11401 agacgatccg catcgtccac tcgtctcacg gtgcgatgct tggaacggag ctggtacgat
    11461 atctcgctca caacgtagtg gccaagccga gcccctccgg agaagtcttg tcgttgctgc
    11521 gttgggccgt gcgtgccgca ggctccgtgg agcgcaacgc gagcagggct tgatggctcg
    11581 ccagctcgca gcggaacgag cgagccttcg cgtgtggcgc agccgtgcgc gacacgtctc
    11641 gctcgtcgcc gactagcgca aaataagacc catgactttg gaagaattcg gcagcagcga
    11701 actctgtcgg tgaacacgtc tcgcgcgctc gagcggtgta gagtgtcctg tcactcgaac
    11761 aagcaggcag aacaatcgag cggggcacaa aaacgccgcg ccggcagcct atccgcaaaa
    11821 gaagggcggt gctgatatcg agtgttcctt tcggtcagtg ccgatcgtga gatgacgtct
    11881 cggcttcgat tcgaagacgg atcggtaaac ctttgatatg ttcctctcgc gacgctggca
    11941 tcgcgtcagg gagcgacttg tcatgagtta cggatttttg cgcggtttgc gcggttcgac
    12001 ctcgcgtgtt tgggagcctc gaagctttat ccaacacgcg tggtttccgt tgctgtgtag
    12061 tgtctggctc accgcgtgtg gctcggacag caacggcacc gccgcgggcg gcagcccgag
    12121 cggcggtgca ggaaatgccg gttccggcgg cagcccgagc ggtggctcca gtggatcagg
    12181 cggcacctcg aatggtgtgg gcggtggcag cgcgggtatg tctggcagca cgagcccaac
    12241 gaacggtgga tcgtcgacca gcggcggcaa tgccgcgggg ggcgattctt cgacgaccgg
    12301 cggcactgcg gcaacgaccg gcgggtctgc gaacggaggc aaaggtggcg gggtgagcac
    12361 cggcggagcc tccgcgggtg gagcgggctc cggcgggaaa ggcggcgcga gcagtgcagg
    12421 ctccggtggc gcgacctcga cctccggcgg cgcgggcggc gctagctctg ggtgtttgga
    12481 gttcgcggcc ccgaccaagg ccggtgacat cgagagcact gcgctcgatg gtccgtcggg
    12541 cattgccg
//
LOCUS       DASTKU010000165         5784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_699687, whole genome shotgun sequence.
ACCESSION   DASTKU010000165 DASTKU010000000
VERSION     DASTKU010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699687"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFQ61_05930"
     CDS             <1..456
                     /locus_tag="VFQ61_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954019.1"
                     /translation="YAVLGAGVIAGAGAAVGLKPSSERLGAVVLCCDFVVGLAFAALG
                     QARQGWGAGALLFAMGGALGLTMVAGTTWFQSRTPGALMGRVMSFVMFAIFGLTPIST
                     FVAGYLLRSYSVGVVTAVAGVTLSCVAAIGLATPKVRRMGFPQLDRSTT"
     gene            703..>5784
                     /locus_tag="VFQ61_05935"
     CDS             703..>5784
                     /locus_tag="VFQ61_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET9954020.1"
                     /translation="MQSKNGHDSKSARPSKDTLAANDAITTREIIGTSDDIAIIGMAC
                     RFPGGVMNPQQYWEFLLTGGDGIVPIPGERWDSKAYYDPDRAKKNRMYVERGGFIGGL
                     DQFDPIFFGISPAEANQIDPQHRWLLEVTYEALENAGLSAAKLRGSDTAVYIGQFMHD
                     YEQLQLDGSAHGMITSHSATGPSMTLTANRISYVFDFRGPSVSLDTACSSSLVALDLA
                     CKALLHGDAELAVAGGVNLLLRPELTMAICKASMLSPDGRCKSFDASANGYVRSEGAG
                     VVLVKRLSEAVRDGNQIWAVIRASGVNQDGHTVGITVPSGAAQEALLRRSLQRAALRG
                     HQIQYAEAHGTGTAVGDPIEVNALGAVLGEERPAEAPFILGSVKSNIGHLESAAGVAG
                     LIKTVLALNHGVIPQNLHYETINPKIDLQALNCRVADRQLPWPETGGERRRAIVNSFG
                     FGGTNANVIVEQAPARVTETGAAADTGVNGPIRALVLSAKTEAALLALAQKYLAFIDA
                     QQAAGEKGASLHEICYSAACAREHHKHRLAVIGASLAEVRGELERFLQGVPSPSRVHG
                     AAALTSVSSADASARLCFVYSGMGTQWLGMGRELYATEPVFRQAFDRCSLAFEKLSGW
                     SLTRRILEQQDEPIDDTAVAQPAIFTIQVALTALLESWGVRPSAVVGHSAGEVCAAYA
                     AGALSFEDSVLVAYHRSRLQAQTEGTGTMLAVGLREGQIGDYLRGIEDCVSVAAINSQ
                     DAITLSGERSALERIHARLEAEGVFARFLNVGVPYHSPVMDRLEQPLLQALREIRALE
                     PRVALYSTVSGKRTQTGDWTAEYWFRNVRQPVLFQAAIESMERDGSVAFLEVSPHAAL
                     ASSVQKNIERLGQVPIVASTSKRNTDDALSLTRAMAALHVQGSSVSWDVMYARGGRLV
                     TLPNYAWQHAAYWYESEAARRARLSNVARSGNLVQSSHPLAGARLNSGSELWQNNVDL
                     NEQAYLADHQLDGEPVFPAAGYVEMALLLAKRAKGEGTDSSALVLEDLDFTRVLFLDR
                     ERATPIEMSPAAPGARELVIRAFDSSSSSWSVHSTATISDSGSGISNHAASPSPARAV
                     DLNGVRERCGKSWERGEFYEHCHRLGLSYRGFFQVVTGARFDGDEVLTELSLPEALLA
                     THADYLLHPVLLDGGFQGLFAVANQGYVPVRIAELRYHRRPPTRLYCHIRITNRTATG
                     ITGDVTLLDEQGVICVEVIGMESTATARPSGAEQPEAALLYAFDWRRYVDADIAEGQA
                     ASATAPGPWIVLADADDLSRGGDAQAIAAELAAAGQEVTIIVPETHPGLVANAGQTSS
                     LIGFDCCADGDLEVVLQSRVRECQGIVFARSLGAAGISVQASSEQMLGACRDALLPAL
                     HLAQALGRLESTVLPRVYFVTRSAQRVDSSDSSLDPTQSALWGFVRVFGSEYPACSAT
                     LVDTDGTPASVAAFVRHLRGGEYEQELAFREGVRFANRLRALHEPELADYAREPYLRA
                     RGPFTWTGSRPSPHAGTTEQTLVFQRAGASAPTAGQANVAINCAVLSKLELEGLLAAN
                     SDAGSQTPGRGYACVATVTELPTDYTGQLRVGDQVVGFVDTPLGSHGQAQAELLVRKP
                     EALSDAEAALLPTAWLSAWYAVSHVARLRADQTLFLYEPSGAGGLAAAQLARLLGVRV
                     LASSCQELVRERLTRFGVELFDSRSP"
BASE COUNT          992 a         1751 c         1920 g         1121 t
ORIGIN      
        1 tacgctgttc tgggtgccgg agtgatcgcg ggcgcgggag ccgcggtagg cctaaaacca
       61 agttcggagc ggctgggcgc ggtggtgctt tgctgcgatt ttgtggtggg ccttgccttt
      121 gccgctctgg ggcaagctcg acagggctgg ggagcgggcg cgctgttgtt cgccatgggc
      181 ggggccttgg ggctcaccat ggtggcaggc acgacttggt ttcagtcacg gacccccggc
      241 gcgttgatgg gccgagtgat gagtttcgtg atgtttgcga ttttcggtct aaccccaata
      301 tccaccttcg tggccggcta cctgcttcga tcatactccg tgggagtggt gaccgcagtc
      361 gctggcgtta ctttatcctg tgtggctgcg atcggactcg cgactccgaa ggtgcggcgc
      421 atgggcttcc cgcaactgga tcgaagcacg acttgagtta attcgatatt tcgcggtcct
      481 ttcggggtcg caaaggtagg actacccagc tcgagatcta ctcggctcag gcgctcagtg
      541 gcgccaagta gctcgaatcc cgaatttctt cgaagcagct ctgctttccg ctcacttgtc
      601 cggcttatcg agccgaaccc gcgaagctgc actcgcatga tcatgcacgc gacccacgcc
      661 ggtgtggacc tcgcttggcc gaatcgacac agggaaactc caatgcaatc aaagaatggc
      721 cacgactcga agagtgcgcg cccgtccaag gacacgctcg cagccaacga cgccatcacg
      781 acccgcgaaa tcatcggaac cagtgatgat atagccatca tcggtatggc ctgccggttc
      841 ccaggtgggg tgatgaatcc gcagcaatac tgggagtttc tgctcacggg cggagacggg
      901 atagttccaa tccccggcga acgttgggat agcaaagcct attacgatcc cgatcgagcc
      961 aagaaaaacc ggatgtacgt cgagcgcggg gggttcatcg gtggcttgga ccaattcgac
     1021 ccgatcttct tcggaatttc tccggcggag gcgaatcaaa tcgatcccca acaccgctgg
     1081 ttactggagg tcacctacga ggctctcgag aatgccggcc tcagcgcggc caagttgagg
     1141 ggcagcgata ccgccgtata catcggccag ttcatgcacg attacgagca gctgcagctc
     1201 gatggctcgg ctcacgggat gatcaccagc cattcggcaa ccgggccgtc gatgaccctg
     1261 accgcaaacc ggatttccta cgtcttcgac tttcgcggac ccagcgtgag tctggatacc
     1321 gcctgttctt cctcgctggt ggcgctcgac ttggcgtgca aagcactgct ccacggtgac
     1381 gcggagcttg ctgtcgcggg tggcgtgaat ctgctgctgc gccctgagct cacgatggcg
     1441 atctgcaagg cttcgatgct ttcgcccgac ggccgttgca agagcttcga cgcgtccgcg
     1501 aacgggtatg tacgcagcga gggcgcgggt gtagtgctcg tgaagcggct gtccgaggcc
     1561 gtgcgggacg gaaaccagat ttgggctgtg attcgcgcct cgggcgtcaa ccaagatggg
     1621 cacacggtgg gaattacggt gcccagcggc gcggcgcagg aggcgctctt gcggcgctcg
     1681 ttgcagcgcg cggcgctgcg cggtcaccaa atccaatacg cggaggcgca tggcacgggc
     1741 acggccgtgg gtgatcccat cgaggtgaac gcattgggag ctgtcttggg tgaagaacgg
     1801 ccagcggagg ctcccttcat cctcggctcg gtcaagagca acatcggaca tctcgagtcc
     1861 gcggcggggg tcgccggttt aatcaagacc gtgctggctc tgaatcacgg agtgattccg
     1921 cagaatctgc actacgagac catcaacccg aagatcgact tgcaagccct gaattgccgc
     1981 gtggccgacc gccagttgcc gtggccagaa acgggcgggg agaggcgccg agcgatcgtc
     2041 aactcgttcg gattcggagg gaccaatgcc aacgtcatcg tggaacaggc ccccgcgcga
     2101 gtgaccgaaa ctggcgctgc ggcggacacg ggcgtgaatg gccccattcg cgcgctcgtg
     2161 ctcagcgcca agacggaagc ggccctgctg gccttggccc aaaaatactt ggcgttcatc
     2221 gacgcgcagc aggcggcggg ggaaaaaggc gcatcactgc acgagatttg ttactccgcg
     2281 gcctgcgctc gtgagcacca caagcaccgg ctagctgtga ttggggcgag cttggcggag
     2341 gttcgcgggg agctcgagcg cttcctgcaa ggagtgccgt caccctctcg ggtccacggt
     2401 gcggccgcgc tgacgtcggt atcgagcgcg gacgcgtccg ctcggctttg cttcgtgtac
     2461 tcgggcatgg ggacccagtg gctcggtatg gggcgtgagc tttacgcgac cgaaccggtg
     2521 tttcggcagg ctttcgatcg ctgcagcctc gctttcgaga agctgagcgg ctggtcactc
     2581 acccggcgca tcctcgagca acaggatgaa cccatcgacg acaccgccgt ggcgcagccc
     2641 gcgatcttta cgattcaagt cgccctcact gcgctgctgg agagctgggg cgttcgcccg
     2701 agcgcggtcg tggggcacag cgccggtgag gtgtgcgccg cctacgcggc gggtgctttg
     2761 agcttcgagg atagcgtgct cgtggcttat cataggagtc ggctccaagc gcagaccgag
     2821 ggcaccggca cgatgctcgc ggttggcctt cgcgagggtc agatcggcga ctacctacga
     2881 gggatcgaag actgcgtctc ggtggctgcg atcaacagcc aggatgcgat tacgttatcc
     2941 ggtgagcgct ccgcgctgga gcgcattcac gcgcgcctgg aagcagaggg cgtgtttgcg
     3001 cgcttcttga acgttggggt gccttaccac agcccggtca tggatcggct ggagcagccg
     3061 ttgctccagg cactgcggga gatccgcgcc ctggagccgc gggtcgcact ctattccacg
     3121 gtgtccggca agcgcacgca gacgggtgac tggacggccg aatattggtt tcgcaacgtg
     3181 cgccaaccgg tgctgttcca ggccgccatc gagtcgatgg aacgcgatgg ttcggtcgcg
     3241 tttctggaag ttagcccgca cgcggctctg gcctcttcgg ttcagaagaa tatcgagcgc
     3301 cttgggcagg taccgatcgt ggccagtacg tcgaagcgca acaccgatga cgccctcagc
     3361 ctgacgcgcg cgatggccgc gctccatgtg caaggctcga gcgtgagttg ggacgtcatg
     3421 tatgcgcgag gcggccgtct cgtgacgttg ccgaattatg cgtggcagca cgccgcctac
     3481 tggtacgagt ccgaagcggc gcggcgcgcc cggctcagca atgtagctcg ctccggaaac
     3541 ttggtgcagt cgagtcatcc gctcgcggga gcccgcctga attcgggctc ggagctctgg
     3601 caaaacaacg tggacttgaa cgagcaagcc tacttggccg accatcagct cgacggagag
     3661 cccgtgttcc cggcggcggg ttacgtcgaa atggcccttt tgctcgcgaa gcgagcaaaa
     3721 ggcgaaggga ccgattcgag cgcactcgtc ctcgaggact tggatttcac ccgcgttctt
     3781 ttcttggatc gcgaacgagc cacgccgatc gagatgagcc cggccgcgcc gggcgctcga
     3841 gagctcgtga ttcgggcctt cgattcgagc tctagcagtt ggtccgttca cagcaccgcg
     3901 accatctcgg acagcggcag cggcatatcg aaccatgctg cgtcacccag ccccgctcgt
     3961 gccgtagatc tgaacggggt gcgcgagcgc tgtggaaaat cctgggagcg cggcgaattt
     4021 tacgagcact gccaccgctt ggggctttcc tatcgcgggt tcttccaagt ggtcacgggc
     4081 gctcggttcg acggcgacga ggtactgacc gagctcagct tgcccgaggc gctgctcgcg
     4141 actcacgcgg attatttgct gcacccggtg ctactcgacg gcggattcca aggcctgttc
     4201 gcggtggcga atcaaggtta cgtgccagtg cgaatcgcag agctgcgcta tcatcgccgc
     4261 ccgcccactc ggctttattg tcacatccgc atcaccaatc gcaccgccac gggcatcacg
     4321 ggagatgtga ccttgctgga cgagcagggc gtcatctgcg tcgaggtcat cggcatggag
     4381 tcgacggcga ccgcgcggcc atccggagcc gagcagcccg aggctgcgct gctgtacgct
     4441 ttcgattggc gaaggtacgt cgatgcggac atcgccgagg ggcaggcagc gtctgcaacc
     4501 gcgccgggtc cgtggatcgt gttggcggat gcagatgact tgtctcgagg aggggacgcc
     4561 caagccatcg cggccgagct ggccgcggca ggtcaggaag tcacgatcat cgttcccgaa
     4621 acgcatcctg gcctcgttgc gaatgctggg cagacaagct ccctgattgg attcgattgt
     4681 tgtgcggatg gagatttgga ggtggtcctt cagtctcgcg tgcgagaatg tcagggcatc
     4741 gtcttcgctc ggtctctggg tgccgcgggc atttccgtcc aagcatcgtc ggagcagatg
     4801 ctcggcgcgt gccgagacgc gctattgcca gcgctgcact tggcgcaggc tttgggccgc
     4861 ctcgaatcga ccgtgctgcc tcgggtttac ttcgtgactc gctccgcgca gcgcgtggac
     4921 tcgagtgact ccagcttgga cccgacccag agcgcactct ggggcttcgt tcgcgtcttc
     4981 ggttcggaat atccagcgtg ttccgctact ctcgtcgata cggatggaac gcccgcctcc
     5041 gtggcggcgt tcgttcggca cttacgaggt ggtgaatacg agcaggagct tgcgttccgt
     5101 gaaggggtgc gctttgccaa tcgtttgcgt gcgctgcacg agccggagct ggcagactac
     5161 gctcgagaac cttacttgcg tgcgcgtggc ccctttacct ggacggggag ccgcccatcc
     5221 ccgcacgcag gtactaccga gcaaacgctg gtcttccagc gcgcaggggc gagcgcaccg
     5281 acggccgggc aagcgaacgt tgcgatcaac tgcgcggtcc tctccaagtt ggagctcgag
     5341 ggcctactcg cggcgaactc cgacgctggg tcccaaacac ccgggcgcgg ctacgcctgc
     5401 gtggcgaccg tgacggagct accgacagac tacacgggac agctgcgcgt cggcgatcag
     5461 gtggtcggat tcgtcgacac tccgctgggc tcccatgggc aggctcaggc cgagctcttg
     5521 gttcgcaaac cggaggcctt gtccgatgcc gaggcggctt tgttgccgac ggcttggctc
     5581 agtgcctggt acgcggtctc gcacgtcgcg cggcttcggg ctgaccaaac cttgttcctt
     5641 tacgaacctt ccggtgcggg agggcttgca gcggcccagc tcgcgaggct gctcggggtg
     5701 cgcgtcctgg cttcctcttg ccaggagctc gtgcgagagc ggctcacgag gttcggtgtg
     5761 gagttgttcg actcccgatc cccg
//
LOCUS       DASTKU010000166         9108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_478238, whole genome shotgun sequence.
ACCESSION   DASTKU010000166 DASTKU010000000
VERSION     DASTKU010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478238"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFQ61_05940"
     CDS             complement(<1..479)
                     /locus_tag="VFQ61_05940"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="trpE(G); catalyzes the formation of anthranilate
                     from chorismate and glutamine; contains both component I
                     and II; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET9954021.1"
                     /translation="MQVIEERYATASGIQVERRATELPLATAIDPLVEALDSQRGMLL
                     ASTYDYPGRYKRWDMGFVNPPLVIEARQRRFRIAALNERGERLIPELQATLAKLAAVE
                     SLERHGGDLQGTIGEPTGRFAEEERSKQPSVFSVLRALVQSFFHPAERYLGLYGSFG"
     gene            complement(636..977)
                     /locus_tag="VFQ61_05945"
     CDS             complement(636..977)
                     /locus_tag="VFQ61_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954022.1"
                     /translation="MAVALDILAEARIRDWQRRKASGECAVQSTGRAAPLAHDAGTLQ
                     TQLLQRVLELLERAARAEPEERAKLDSTARELETRLMVLLEDEGLGLAARRIAAQLEQ
                     RRAGWGQRSQP"
     gene            complement(1125..3596)
                     /locus_tag="VFQ61_05950"
     CDS             complement(1125..3596)
                     /locus_tag="VFQ61_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9954023.1"
                     /translation="MRERGPTCLFGVAGPSAATPRRIHWRPRWVKVYRDVLTERGRIA
                     LMLIAIAVSLMGIGAVLGAYGILTREMARNYLGTRPASASLELSAAVDHELLEAVRKR
                     SGIVEAAAGEIVLARAKVGHDWRPLLLFVVRDFGDLRLNTFRHQSGAWPPPTGSMLIE
                     RTAKQVLEAGEGSSVWVKTPHGAPREVPIVGVVHDPGLAPAWQEAEGYGYITPATLAA
                     LGESAELRELRILVGDRPFERAAVDARVVETSRWLRERGVRVVEARVPPPGKHPHQTQ
                     MYGVLFLMLVFAGLTLVLSGVLVATSLAGMLARQVREIGIMKTLGASRAQLVALYAVL
                     VAALGITAFLVALPSGVMGAHALARMSGRMLNLELASLAIPSWVFAVQGFSGVVVPLL
                     AAAVPIAGGTRATIRTALDANGAVVPRAVRLARIAVLFGRIFSNRVLAMAVRNAVRRR
                     ARFVLTLLLLAMGGAMFQSALNISRGWQRIVARVYENRSYDVEVRLQEPVAPAVVEGV
                     PGVRRVEAWGYLRTALWKPQGVDIVRTYPDGSHGSLVLMAPPLGTELVRFPLLSGRWL
                     EPGDGDGIVLNHMVLNQTPGTRVGDSITISVRGEPTTWRVVGIVEEVGSPGAVYVNPA
                     AFSRLSGVPESQSRMLRIATQAGSPGERTHIIRRLEDRLAEQHVPVESVIPLSVLRSA
                     MGDHVIVLIRMLLAMAGLMVIVGGLGLATTMSTSVIERTREIAVLKTLGATPWSVARL
                     VVTEALVVALTSWVVAGGVAVPLTVAIGRTVGQLSFRVSLPFEADPSAVFAWLVFVAC
                     LAVLATVLPAWRAARLTVREALMLV"
     gene            complement(3589..4266)
                     /locus_tag="VFQ61_05955"
     CDS             complement(3589..4266)
                     /locus_tag="VFQ61_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9954024.1"
                     /translation="MAGRSFTALESLDLRLPGGALTAIVGKSGSGKSTLMNLIAGLDR
                     PDSGRIVVSGTELQRLDESELARWRGRHVGVVYQFFQLLPTLTVLENVTLPIDLCSTL
                     PARRARDRAYELLERVGVRDQAEKLPSALSGGQQQRVAIARALANDPPLIVADEPTGN
                     LDSKTAGDVLELLVDLTRLGKTLIVVSHERDVRRVAYRVVTLADGRIESDEISGRASQ
                     QVEVSHA"
     gene            4458..5120
                     /locus_tag="VFQ61_05960"
     CDS             4458..5120
                     /locus_tag="VFQ61_05960"
                     /inference="COORDINATES: protein motif:HMM:NF025636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9954025.1"
                     /translation="MSVKANSRPDRRIQRTRRVLHSALIELILERNFDELTVQDVCER
                     ADVGRSTFYTHFADLEDLLLSGFDELRGVLRAVPDDAEPNLAPLPGAQRSGSQRSGSQ
                     PAVPHTPAPLAFSRDLIAHTHENRRLFRAMVGKRSGRAIQKRFRQLLLDLIQEDLASV
                     LPAGAQREATAHYLTGAFFELLMWWHETRNSFSTEELDTLLHRLTGSALASLGVSLGR
                     RD"
     gene            5451..7016
                     /locus_tag="VFQ61_05965"
     CDS             5451..7016
                     /locus_tag="VFQ61_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9954026.1"
                     /translation="MEILVTGGTGVVGSPTIAALVRREHRVRLYSRHASESKRLWPHH
                     VSTVDGDIEDEEALTRTMAGADAVIHLAGIVSERDGKTFETVNVEGTRHALRAATRAH
                     VKRFVYMSSLGAERGQSPYHQSKRMAEELTRGFEGEWVVLRPGNVYGPGDEVISLLLK
                     IIRVSPVVPAIDGGDHRFQPIWVEDLAEATAEAVTRADVVQRSLDLAGPDLTSMNDLI
                     DRFRSLTGRSPVRVPVPGFLSVLGARAAGLLGMNVPLDRGQVQMLEEGNLIAEPADNA
                     LVSVFAIKPTPLDEGLRRLADSLPEQPLDSGVGSMVRRHVWADIVESQLDAEALFERF
                     RARFGEITPWHVEVGAEPGTPVDPRLGATLTMHLPLRGNVQVRVVELTKSYLTLATID
                     GHPLAGLVRFRLEPLGERRIRFHVEVHDRPGNIADWLVMSTVGGTIQVATWRSTVEHL
                     VKESGGSAPDGVHDESENLHGEEAAQVESWARGLIEARKRSLHEAGLSEATSPDKSRA
                     QQAPASVRAETRS"
     gene            complement(7037..8260)
                     /locus_tag="VFQ61_05970"
     CDS             complement(7037..8260)
                     /locus_tag="VFQ61_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9954027.1"
                     /translation="MSLPRLLASEHLRDVKYEIRGALARRADELEREGHDIIKLNIGN
                     PGAFGFRMPSTMRVAMVQNLHQADPYSHQKGIFQAREAVVMQQQDRGVMDVTADDVFI
                     GNGVSELIMLTMRALLNPGDEVLVPSPDYPLWTASVVIHGAKAVHYPCRPEQNFVPDP
                     KEIRALITDKTRAIVVINPNNPTGAVYPESVLKELAAIAEEHHLVMCADEIYDEMLYE
                     DARYVPMATLAKHTLCLTFSGLSKIYRACGLRVGWVVFSGERAHARAYIEAVELLASL
                     RLCSNVPGQFAVQTALGGHQSIVELTRPGGRLFETRRQILESVAKSKYLSVVAPRGSM
                     YAFIRIRSELFKGPFDDRAFALQLLEQKHVLVAPGSSFNVPYHDHFRTTFLPDPETMA
                     DVMSRIESLLVERAA"
     gene            complement(8510..>9108)
                     /locus_tag="VFQ61_05975"
     CDS             complement(8510..>9108)
                     /locus_tag="VFQ61_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HET9954028.1"
                     /translation="QASQGYARIKKLVTVLADKLRGQGDIPLSPGLYASQRSEAEQVP
                     RMRHHYVHGQFSAHTLEFLGRFRSAMDDDLNTPNAVAALFDYVSALYASGIEQGTELP
                     SLLAVYRCLTRHLSVFGVEIGEAALYPELIGDYAQGEQAMAQGRGGDTVLDRMLAMRT
                     AARAAKDFAKADAIRNLLTEAGVALEDTPAGPRWSVGK"
BASE COUNT         1681 a         3050 c         2822 g         1555 t
ORIGIN      
        1 tagccgaagg agccgtaaag cccgagatac cgctctgccg gatggaagaa cgactgaacc
       61 aaggcacgga gcaccgagaa caccgagggc tgtttgctgc gctcctcctc cgcgaagcgc
      121 cctgtgggct ccccgatcgt accctgcaag tctccgccgt gacgctccaa gctctccacg
      181 gccgcgagct tcgcgagcgt cgcctggagt tcgggaatca gccgctcccc gcgctcgttc
      241 aacgcggcga tccggaatcg gcgctgacgc gcttcgatca cgagcggcgg gttcacgaag
      301 cccatatccc aacgcttgta acgacccgga tagtcgtacg tcgacgcgag cagcatgccg
      361 cgttgactat cgagagcttc gaccagggga tcgatggctg tggcgagagg cagctcagta
      421 gcgcgtcgct cgacctggat gccgctggcg gtggcgtacc gctcttcaat gacttgcatt
      481 ttcgcccaat ccttttgccc tttcttcccc gctcgcgcaa cccgacccag gccccttcat
      541 cacggagcaa gacgggcgag cactatccga acccgcggcc tttcagcaga actccctcac
      601 ctaccgagtc cgcgaccgag cggagtgcct cgtgatcagg gttgcgagcg ttggccccaa
      661 ccggcgcgcc gctgctcgag ctgtgcggcg atgcggcgcg cggccaggcc gagcccttcg
      721 tcctccaaca gcaccattag ccgggtttcg agctcccgag ccgtactgtc gagctttgcg
      781 cgttcttccg gctcagcgcg tgccgctcgt tcgagcagct cgagcacgcg ctggagcagc
      841 tgcgtttgta gtgtccccgc gtcgtgcgcg agcggcgcgg cgcgtccggt ggattgaacc
      901 gcgcactcac cgctcgcctt acgacgttgc cagtctcgga tgcgagcttc agccaggata
      961 tcgagagcga ctgccatgtc tcaaaactag cctcggggcg gcaataccgc ccgcaactct
     1021 ttcataggag cctccgctct gtcatcggag cctccgctct gtcatccgag cgtccgctct
     1081 gtcatccgag ccttcgctct gcgttggggg gggcggacgc tctgtcatac caacatgagc
     1141 gcttctcgaa cggtgaggcg ggccgctctc caggcgggca acacggtcgc aagcaccgcc
     1201 aggcacgcga cgaacacaag ccaagcaaac accgcgctcg gatccgcttc gaacggaagg
     1261 ctcactcgga acgacagctg ccccacggtt cggccgatcg cgaccgtcaa cggcacggcc
     1321 acgccacctg ccaccaccca gctcgttagg gccaccacca gggcctccgt caccaccagt
     1381 cgcgcgacac tccacggggt agcacccaag gtcttcagaa cggcgatttc gcgcgtacgc
     1441 tcgatcacgc tggtgctcat cgtggtcgca agccccaacc cgcccacgat gaccatcagc
     1501 ccggccatgg ccagcaacat gcgaatcagc acgatgacat ggtctcccat cgccgaacgc
     1561 aacacggaga gcgggatgac ggactcgacc ggtacatgct gctccgcgag tcgatcttcc
     1621 aagcgtcgaa tgatgtgcgt gcgctcgccc ggagaccccg cctgcgtcgc gatccgcagc
     1681 atgcgcgact gcgactcggg aacgccactc agacgcgaga aagcagccgg gtttacgtag
     1741 accgcccccg gggaaccgac ttcttccacg atccccacca cacgccaggt ggttggctcg
     1801 cctctcaccg aaatcgtgat cgaatcgcca actcgggtgc cgggggtctg gttcagaacc
     1861 atgtgattga gcacgatgcc gtccccatcc cccggctcca gccagcgtcc ggacaacagc
     1921 gggaaacgca cgagctccgt gcccagtggg ggcgccatca acaccagact gccgtggctg
     1981 ccgtcggggt aggttcgcac gatgtcgacc ccctgaggct tccaaagcgc cgtgcgcaga
     2041 tagccccatg cttcgacgcg acgaacgccc gggacccctt cgaccaccgc tggcgcaacc
     2101 ggctcctgca atcgcacctc gacatcgtag gatcgattct cgtaaacccg agctacgata
     2161 cgctgccagc ctctcgaaat gttgagtgcg ctctgaaaca tggcgcctcc catcgccaag
     2221 agcaggagcg tcaaaacgaa gcgcgctcgg cgtcgaactg catttcgcac agccatcgcg
     2281 agcacacggt tggagaagat acggccgaag agcactgcga tgcgcgccag ccggacagcg
     2341 cgcggaacaa cggcaccgtt ggcatcgagc gccgtccgga tcgtggcccg cgtgccaccg
     2401 gcgatcggaa ccgcagccgc gagcagcggc accaccacgc cggagaagcc ctgtaccgca
     2461 aacacccagc tcgggatggc caagctcgcc agctcgagat tcagcatgcg cccggacatg
     2521 cgcgctagcg catgcgcgcc catgactccg gacgggagcg ccaccaagaa cgcagtaatc
     2581 cccagcgccg cgacgagcac cgcgtacagc gcgaccaact gggcgcggct cgcgcccaag
     2641 gtcttcatga ttccgatctc gcgcacctga cgtgccagca tgcccgccag agaagtcgcg
     2701 acgagcaccc cactcaacac cagtgtcaga ccggcaaaca ccagcatgag aaacaacact
     2761 ccgtacatct gggtttgatg cgggtgcttg cccggcggcg gcacgcgcgc ttccacgacg
     2821 cgcacgccgc gctctcgaag ccaacgcgag gtctcgacca cgcgagcatc caccgcggcg
     2881 cgctcgaagg gccggtcacc cacgagaatc cgcagctctc gcagctccgc gctctcgccc
     2941 aacgccgcca acgtagccgg cgtgatgtac ccatagccct cggcttcttg ccaggcaggt
     3001 gcaagcccgg gatcgtgcac gacgcccacg atcggcacct cgcgcggggc accgtgagga
     3061 gtcttcaccc agacgctcga tccctcaccc gcctccagca cctgtttcgc cgtgcgttca
     3121 atcagcatgc tcccggtagg cggaggccaa gcgccgctct ggtgtcgaaa ggtgttcaac
     3181 cttagatcac cgaagtcccg caccacgaac aacaacagcg ggcgccagtc gtgtccgact
     3241 ttggcgcgag ccagcacgat ctcacccgct gcggcctcga cgatgccaga gcgttttcgg
     3301 accgcctcca gcagctcgtg atctacggcg gcgctgagct cgagtgaggc ggatgcgggc
     3361 cgcgtaccta ggtagttgcg ggccatttct cgggtcagaa tgccgtacgc gcccagcacc
     3421 gccccgattc ccatgaggct caccgcgatc gcgatcagca tgagagcgat acgcccgcgc
     3481 tcggtcagga cgtctcgata gactttgacc caacgtggcc gccagtgaat ccttctcggc
     3541 gtcgcggcgg agggccctgc gaccccgaac aagcacgtcg gcccccgctc acgcatgact
     3601 gacctcgact tgctgcgaag cacgtccgga gatttcgtct gactcgatgc ggccgtcggc
     3661 gagcgtgacc acccggtacg ccacgcgccg cacgtctctt tcgtggctga ccacgatcaa
     3721 ggtcttaccg agtcgcgtca aatccaccaa caactcgagc acgtcacccg cggtcttcga
     3781 gtcgaggtta cccgtgggtt cgtccgccac gatcaacggc gggtcgttgg ccagcgcccg
     3841 agcgatcgcg acgcgctgct gctgaccacc ggacagcgca ctcggcagct tctccgcttg
     3901 atcgcgcacg cccacccgtt ccaacaactc gtaagcccga tcgcgggcgc ggcgcgcggg
     3961 aagcgtcgag cagaggtcga tcggcagcgt cacgttttcg agcacggtga gcgtcggaag
     4021 cagctgaaag aattgatata cgacgcccac gtggcgccct cgccaacgtg cgagttcact
     4081 ctcgtctagc cgttgcagct cagttccgga aaccacgatg cgacccgagt ccggccgatc
     4141 gagccccgcg atcaagttca tcaaagtgga tttgccgctg ccggacttgc ctacaatcgc
     4201 ggtgagcgcg ccgccgggta gcctcaaatc gagcgattcc aaggcggtga agctacgccc
     4261 cgccacgggg tagctcttag tgacggagtg gagttcgacg gcgtggacag cgggataatt
     4321 attggacaca gtgtccatat tgcgagccca cgcattacgt tcaatcgaca gccccccggg
     4381 cattggccag ttccggacat tcggggcggc actgtccgga atctcgtcgc gaagcgggtc
     4441 tccagcgaaa gcctgccatg tccgttaaag ccaactcccg accggaccgc cgaattcaac
     4501 gcacgcgtcg cgtgctgcat agcgcgctca tcgagctgat cttggagcgc aacttcgacg
     4561 agctcacggt tcaagatgtc tgcgagcgag ccgacgtcgg tcggtccacg ttctacacgc
     4621 acttcgccga tctggaggac ctgctcttga gtggcttcga tgagctgcgc ggcgtgttgc
     4681 gcgctgttcc tgacgatgcg gagcccaacc tcgcgccgct ccccggggca cagcgctcgg
     4741 gctcacagcg ctcgggctca cagcccgcgg tgcctcatac accagcgccg ctcgcatttt
     4801 cgcgtgacct catcgcgcat acccacgaaa accgccgcct gtttcgggcc atggtcggta
     4861 aacgcagtgg ccgtgccatt caaaaacgtt ttcggcaatt gctgctggac ctgattcagg
     4921 aggatctcgc gagcgtgcta cccgcaggcg cgcagcgcga agcgacggcg cactacctga
     4981 cgggcgcgtt cttcgaactg ttgatgtggt ggcacgaaac ccgtaactcc ttctcgacag
     5041 aagagctgga tacgctgctc catcgattga cgggctctgc tctggcgtcg ctcggtgttt
     5101 cactcgggcg gcgtgattga gtgccgttgg cgcggtggcg tgggaaacgg gggcggcgcg
     5161 actgcgctct tgggctgagc ggtgctggat ggcgcgctcg ttatcgcggg cggcaggcaa
     5221 gcctcgcttg cgatcccgac caatgcgttg cggctgacct cgaggtcgag atcgcaggag
     5281 ccagtcgagc gtcaattcgc ccaaagaacg cgacatttcg acacgcaatc agagcgcaaa
     5341 ctgagctcat gtgtcgcggc tgacaacact cttcaacgag cgtggttaga catgcaattt
     5401 gcggcgtaga cactcgggcg cgactactca cgacaagcta gaagacaggc gtggaaattc
     5461 tagtgactgg cgggaccggc gtcgttggct ctccaaccat cgccgctttg gtgcgtcgcg
     5521 agcatcgggt acggctctat tctcgacatg cgtcggagtc gaagcgattg tggccgcacc
     5581 acgtcagtac ggtcgacggg gatatcgagg acgaagaagc cttgacccgc accatggccg
     5641 gggcggatgc cgtaattcac ctagccggca tcgtcagcga acgcgacggc aagacgttcg
     5701 aaaccgtcaa cgtggaaggc acgcggcacg cgttgcgagc ggccacgcga gctcacgtca
     5761 agcgcttcgt ctatatgtcc tcgctcggcg cggagcgcgg acaatcgccg tatcaccagt
     5821 cgaagcgtat ggcggaggag ctcacgcgcg gcttcgaggg cgagtgggtg gtgctccgcc
     5881 ccggcaatgt ctatgggccc ggcgatgagg tgatctcgct gcttttgaag attattcggg
     5941 tctctccagt ggtgccggcg attgatggcg gcgaccaccg ctttcaaccg atctgggtgg
     6001 aagatctggc cgaggccacg gcggaggcgg tgactcgcgc cgacgtggta cagcgctcgc
     6061 tcgacctcgc ggggccggat ctgacgtcga tgaacgattt gatcgatcgc ttccgaagcc
     6121 tgaccggtcg ttctccggtg cgcgttccgg tgcccggatt tttgagcgtg ctcggcgcgc
     6181 gtgccgcggg gctgctcgga atgaatgtgc cgctggaccg cggccaagtt caaatgctcg
     6241 aagagggcaa cctaattgcg gagccggcgg ataacgcgct cgtgtccgta ttcgcaatca
     6301 aacccacccc gctcgatgag gggttgcgtc gcctcgcgga cagcctgccc gagcaaccac
     6361 tcgattcggg cgtgggcagc atggtgcggc gccacgtctg ggccgatatc gtggagagtc
     6421 agctcgacgc agaggctttg ttcgagcgct tccgcgcccg tttcggggaa attacgccgt
     6481 ggcacgtgga ggtgggcgcc gaaccgggta ctccggttga tccgcgtctg ggcgccacgc
     6541 tcaccatgca cctaccctta cgcggcaacg tgcaagtgcg ggtggtggag ctcaccaaga
     6601 gttacctgac gctcgcgacg atcgatggcc atccgctcgc aggtctggtt cgattccgtt
     6661 tggagccact gggtgagcgc cgcatccgct tccacgtcga ggtgcacgat cgaccgggca
     6721 atatcgccga ctggttggta atgtccaccg tgggcggcac cattcaagtg gcaacctggc
     6781 gctcgacggt agagcacctc gtaaaagaga gcggcggctc cgctcccgac ggcgtgcacg
     6841 acgagagtga gaatctacac ggtgaagagg ccgcccaagt ggagagttgg gcgcgcgggc
     6901 tgatcgaagc gcgcaagcgc tccctgcacg aggccggcct gtccgaagca accagcccgg
     6961 acaagagccg cgcgcagcaa gccccagctt ccgtccgcgc ggagacccga agctagatat
     7021 ggatgacgcg agctatctag gccgctcgct cgacgagtag agattcgatg cgggacatca
     7081 cgtccgccat cgtctcggga tcgggcaaaa acgtcgtacg gaagtggtcg tggtacggca
     7141 cgttgaagct cgatccgggc gccaccagca cgtgcttttg ctccaggagc tgcagcgcga
     7201 acgcgcgatc gtcgaacggg cctttgaata gctccgagcg aatgcgaatg aacgcataca
     7261 tggagccgcg cggagccacc accgacaggt acttgctctt cgcgactgac tccagaattt
     7321 gacgccgagt ctcgaacaac cgtcccccag ggcgcgtcaa ctcgacaatc gattggtgcc
     7381 cgcccagcgc ggtttgcacc gcaaattgcc cgggaacatt ggagcataaa cgcaacgacg
     7441 cgagcagctc caccgcctcg atgtaggcgc gggcgtgagc tcgttcaccc gagaacacca
     7501 cccatccgac gcgcaacccg caggcgcggt agattttgct caggcccgag aacgtcagac
     7561 acagcgtgtg cttggcgagg gtcgccatcg gcacgtagcg cgcgtcttcg tacagcatct
     7621 cgtcgtagat ctcgtcggcg cacatcacga ggtgatgctc ctccgcgatc gccgcgagct
     7681 ccttgagtac gctctccgga tacactgcac cggtggggtt attgggattg atcaccacga
     7741 tcgcgcgcgt tttgtcggtg atgagggcgc ggatctcttt gggatcgggg acgaaatttt
     7801 gctcggggcg acacggatag tgcaccgcct tggcgccgtg gatcacgacc gaagccgtcc
     7861 aaagtggata gtccgggctc ggcaccaaca cctcgtcacc cggattcaag agggcgcgca
     7921 tcgtcagcat gatcagctcg ctgacgccgt ttccgatgaa cacgtcgtcg gccgtgacgt
     7981 ccatcacgcc gcgatcctgc tgctgcatca cgaccgcttc gcgcgcctga aaaatgccct
     8041 tttgatgcga gtacgggtcg gcttggtgca ggttctgcac catcgcgacg cgcatcgtgg
     8101 acggcattcg aaagccgaag gcgcccggat tgccgatatt cagcttgatg atgtcgtgcc
     8161 cctcgcgctc gagctcgtcg gcgcgccgcg cgagagcccc acggatctcg tacttgacgt
     8221 cgcgcaggtg ttcggaagcg aggaggcggg gcaggctcat ggcgatcacg gctgaactgc
     8281 gccgaatgta gttcgccccg gcggaaatgc cacgtccgag taagctgccg gagctgattg
     8341 tggcgctcca aatgacgggc gcgctgcgcg gcgcttttcg actctgcgcc gcgcactggc
     8401 cccctctacc gccggaagca gccgcgccgg cgccacctct cgcaccccgg caccgcaacc
     8461 acaccggaga accgcggcgc cccgtggcac gcgcgcctgc ttggcccgcc tacttgccga
     8521 cgctccagcg cggacccgcc ggagtatcct cgagcgccac ccccgcttcg gtgagcagat
     8581 tgcggatcgc gtccgctttg gcgaaatcct tcgccgcacg cgctgcggtg cgcatggcga
     8641 gcatgcgatc gagcacggtg tcgccaccac gaccttgggc catagcttgc tcgccctgcg
     8701 cgtagtcccc gatgagctcc ggatagagcg cggcctcccc gatctccacc ccgaacacgg
     8761 agaggtgccg ggtcaggcag cgatagacgg ccagcaacga tggcagctcc gttccctgtt
     8821 cgatgccgct tgcgtagagc gcgctgacgt agtcgaacaa cgccgcgacc gcgttcggcg
     8881 tgttcaggtc atcgtccatg gccgagcgga agcgaccgag gaattccagg gtgtgcgcgg
     8941 agaactgacc gtgcacgtag tggtgacgca tgcgcggcac ctgctcggcc tcggaacgtt
     9001 gtgaggcgta gagcccaggg ctgagcggga tgtcgccttg tcccctcaat ttgtcggcca
     9061 acacggtcac gagcttcttg attcgcgcgt agccctggct ggcttgcg
//
LOCUS       DASTKU010000167         8024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1146445, whole genome shotgun sequence.
ACCESSION   DASTKU010000167 DASTKU010000000
VERSION     DASTKU010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8024
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146445"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2371)
                     /locus_tag="VFQ61_05980"
     CDS             complement(<1..2371)
                     /locus_tag="VFQ61_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9954029.1"
                     /translation="MNSWRRSQYLPVSLFILGSVACGSSPEVTANDPGGLDGRGGLGG
                     GSSTAETGGSGARPGTGGAIPTGGTQPVPQAGAAGDDGSGGAAEDPCASLNCGAGQRC
                     DTRESDPRCVDNTCSDLNCSALEECMPASGGGSICASIACDSDVDCGDSRYCDGTKCV
                     ADVCVPETRVCDGNAVRLCASNGGGEQEAYTCRSAGYFESECSNGAGASKMATGCTCE
                     GDWDCPEYTHCEAGVCKGTGFAPTCTLAPARFEDVLPKLEFRWGGKSGAEPVATGRAF
                     PWSAQVASTPIVINLDDDNQDGLINELDFPEILFMSYDGSAPEQRGTVRAIHGGGPNK
                     GQDYFALCGTTLWQEGKPAATDCNPADTDAQSKSAALARAGGVLAAGDLDSDGRPEIV
                     VPLASGGVQILDNTGKVLTTSAANLWPSLTNTQDWRYPAPAIANLDFEGLAEIVVGNR
                     VLTLARDAEGKLEIARVFTGEFRTGTMHHGSDEQHHGPTVCLADLAPVPGLEIVAGTS
                     LYRLPDAADCSKAPNSDYCLGRLTSVWNAETANPGSSFYAEGFCAVADVLGAETGSAP
                     GPDNPLDREPEVLVIADGHLLILRAKDGLLLRNIDLGGGKQGGAPNVDDFDGDGFPEV
                     ATALSDFYSVTDLQRPDAEHCPAWPSKLSKSDVPPGSNPERDPGGACTRNEDCNSGAV
                     CNTKAGSCVCLHSGWKRLTEDDSSKVTSSSVFDFNGDGAAEVVYNDECYFHVYDGATG
                     GLYLALPSLSRTLIENPVVADVDNDGNAEIVFVQNNETQQCDEAKLDSWP"
     gene            3058..5268
                     /locus_tag="VFQ61_05985"
     CDS             3058..5268
                     /locus_tag="VFQ61_05985"
                     /inference="COORDINATES: protein motif:HMM:NF039970.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6055 domain-containing protein"
                     /protein_id="HET9954030.1"
                     /translation="MSRSSSRAPLLSFVFLALGCGDTGSVTPAPWGGGAGGVGAAGSG
                     GSLSTGAGFGGFSTGPAGGAGTSSSGGAGSGAQNSGGAPGPSSPSSGGTSNRGGVPST
                     NPGTSGSVTGGLGGNGNSSGATGLGGIAGTGGSASGGQGTAGSNAVTGGGPNGGAGTC
                     DPGSTATQWATNCPTSPPACTAGTWIAGGPDPDHSNYKLIKESEHFAIYSDESVSDAN
                     ATSALDTLESKIWSVYFGNPIYFKEPLCNQTTKYKASVHVHSTNGLTGGAWAANRMGM
                     WIGPGALTDHWGLAHEFMHGVQSVSGGMSCGSGPNYCGWIYESHANFMPHQIAEYRGD
                     VHCSEMSFNMPHLYLGSTRDRYCNWQFMEYLKDKYCYSAVNAIWTQSAANDPFTNIMK
                     GMGWNISQLNDFIGEWATHNVTWDYKNPAPTLAGDTLDPGANFRSKYGLITDTSQTIR
                     RLRTTKLDPLDANWAMTRRFASPFYWAPQRFGYNVVRLYPDAGATTVRVSFRGVTTGA
                     PNPDWRWGLVATNSTLTTARYSALQKGADGELSFCVSPGESIWLVVAATPSVQQQIKW
                     DQPYGTVPRYPYLVAFGNAWPDGFQDGKQAACPNGLVRVANGGGCGPANLPASVYVGP
                     YATVLSGATVSGEARIEDHAVVARGTVSGGTVGAMTLIDNFKMSSGTAKSTFYPLGFF
                     ETGQGLSGGTLIGDVEYRGAGLSRTSGTCSGFVDDGTCIAPGADTTATPPFTWR"
     gene            complement(5340..6650)
                     /locus_tag="VFQ61_05990"
     CDS             complement(5340..6650)
                     /locus_tag="VFQ61_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954031.1"
                     /translation="MGSCPETLLGRVSVTDVPLDHDIRYKRPAYDLIPQDERLALAVG
                     PDGLAQVAWLENELTRVHVTPLTAAGNRRDEDSIVPARDIGGLVLHSDGFALLTRRDD
                     PGEPLRDLNPTTPTLATAAVLLRYRNGQEEFAAPLTGTAQLDPNLSEAEKRDCAPRPF
                     LSRLVYNGQKYGAYFTVHGCEGHPRERYYADKLSYVDDQGGFVPGGWAWNCSINEGVR
                     LLPEAGAFTSLCMAVTFPAPGLNLVIGGRASVPLAEEYTTGAYSGGEFGSVVKLGDGS
                     YFVGWLSRGENPGQTPADIVFQLLGPDYRPRIPTTRLSETPTVAEKNLHFARYGSDKL
                     LMVWDSVEELRWINDQTCFGTYTGTHLRLIDLAGNPVSDDEVIAVPPNAEDEIVVYPN
                     GDLGWAFVREDARGYAGPLRSDRDGVPDVPTKRLLSIVRMRYCQ"
     gene            complement(6827..7882)
                     /locus_tag="VFQ61_05995"
     CDS             complement(6827..7882)
                     /locus_tag="VFQ61_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954032.1"
                     /translation="MRFLAIVFGICGLVSFAEAAGAATPERAPASQKVALVRPESPSG
                     ASDTLLLDACNRLRAELELQHFETEVVSAPLGEHTADSLTEIARKVGAIAGIALVHRE
                     PLSSTAVEIWLVDRISGKTTLRRIELGRTPDASSVLAIRAVDLLRASFEEFQSVGEPP
                     PEVAEVDRRPPPPAVRQFATSELRSYAVAIQGVGLLNGPTLGASYGPALGVARCFADF
                     ELWLLAAGPLVGADFEASQGGASSRQEMAWLEARGWLMGTRDFHAGLGAGLGGHFLQA
                     EGQAEPPLRSRSDSTWGFVGLISAHAQLQLSSRLALGFSLRALALMPRQGIAVLDERV
                     KLQQPLLSSTAGVVIGL"
BASE COUNT         1442 a         2602 c         2514 g         1466 t
ORIGIN      
        1 tcggccaact atcgagcttg gcttcgtcgc actgctgggt ctcgttgttc tgcacgaaca
       61 cgatttcggc gttgccgtcg ttgtccacgt ccgccaccac ggggttttcg atcaaggtgc
      121 ggctcagcga aggtagggcc agatacaatc cgcccgtggc gccatcgtag acgtggaagt
      181 agcactcgtc gttgtaaacg acttcggccg caccatcgcc gttgaaatcg aatacgctgg
      241 atgacgtcac tttgctggaa tcgtcttcgg tcaggcgctt ccagccgctg tgcaggcaca
      301 cgcagctgcc agctttggtg ttgcagacgg cgccgctatt gcagtcttcg ttgcgggtgc
      361 aggcgccgcc tgggtctcgc tcgggattgc tccccggcgg cacgtcactc ttgctcagct
      421 tgctgggcca ggccgggcaa tgctcggcgt ccggccgctg caggtccgtc acgctgtaga
      481 agtccgagag tgcggtggca acctccggga acccgtcgcc gtcgaagtca tccacgttcg
      541 gtgcgccgcc ctgtttaccc ccacccaagt cgatgttgcg cagcaacagg ccgtccttcg
      601 cgcgcaggat gagtaggtga ccgtctgcga tcaccagcac ctccggttca cgatccaggg
      661 gattgtcagg gcccggcgcg ctccccgttt ctgcgccaag cacgtccgcc accgcgcaga
      721 agccttccgc gtagaacgag ctccccgggt tggcggtctc ggcgttccag acggaggtga
      781 gtcggcccaa gcaatagtcg ctgttgggcg ctttgctgca gtccgccgcg tccggcagcc
      841 ggtacaagct ggtgccggcc acgatctcta ggcccgggac aggggctaaa tccgccaagc
      901 acacggtcgg gccatgatgc tgttcgtcgg agccatgatg catggtgccg gttcggaatt
      961 caccggtaaa cacccgagcg atttcgagtt tcccttccgc gtcacgtgcc agggtgagga
     1021 cgcgattgcc gacgacgatt tcggctagtc cttcgaaatc caagttagcg atcgcgggag
     1081 cgggatagcg ccaatcctgc gtattggtga gtgatggcca aaggtttgcg gcgctggtag
     1141 tgagtacctt gccggtattg tccagaatct gaactccgcc cgacgcgagc ggtactacga
     1201 tttcggggcg accatccgag tccagatcgc cggcggcgag cacgccgccc gcgcgagcca
     1261 gcgcggcgct cttactttgt gcatcggtat cggccggatt gcagtcggtg gcggcgggct
     1321 ttccctcctg ccagagcgtg gtgccgcaca acgcgaagta gtcctgacct ttgttggggc
     1381 cgccgccgtg aatggcgcgc accgttccgc gttgctcggg cgcactgccg tcataactca
     1441 tgaacaggat ctctgggaaa tccagctcgt tgatcagacc gtcttggttg tcgtcatcta
     1501 gattgatgac gatgggcgtg gacgcgacct gggccgacca ggggaacgcg cgccccgtcg
     1561 ccaccggctc ggcgccgctc ttgccacccc acctgaactc cagtttggga agcacgtctt
     1621 cgaagcgagc gggagccagg gtgcaggtcg gggcgaaccc cgtccccttg catacccccg
     1681 cctcacagtg cgtgtactcc ggacagtccc agtcaccctc gcaggtgcag cccgttgcca
     1741 tcttggaggc tccggctcca ttgctgcact cgctttcgaa gtagccggcg ctccggcaag
     1801 tgtaagcctc ctgttcccct cctccgttgg acgcgcacag ccgaaccgca ttaccgtcgc
     1861 agacccgcgt ttccgggacg cacacgtcag ccacgcactt cgtcccgtcg caatagcgag
     1921 agtcgccgca atctacgtcg gagtcgcagg cgatgctcgc gcaaatcgag cctcctccgg
     1981 atgcgggcat gcactcctcg agcgcagaac aattcaggtc actacaggtg ttgtcgacgc
     2041 acctgggatc actctcgcgg gtatcacagc gctgccctgc accgcagttc aggctggcgc
     2101 agggatcctc cgccgcgcct ccgctgccat catctcctgc agcgccggct tgaggcaccg
     2161 gttgagttcc gcccgttgga atggccccgc cggtgccggg gcgcgcaccg ctgccaccgg
     2221 tctcggcggt ggaagagcct ccgccgaggc ctccgcgtcc gtcgagccca ccgggatcgt
     2281 tggcggtgac ttcgggggaa gaaccacaag ccacactgcc gagtatgaat aggctaactg
     2341 ggagatactg agatctccgc caggaattca tgtgccgaga ttagcaggct gactgcggta
     2401 cgtcacattg ggagacactc gttggccaca tttctgagcc acgtccgagt gcggtatatg
     2461 gcgcgaaggg tgagaggttg gagggtatcg atcgcgtcga gttccggggg gagccatttg
     2521 tcaccctgca gttgagtccg cgtgacggtt cggtttcgtt cacacgctga aagccacgag
     2581 ttcttgcgga ctcggtgaca gcgatgattc gattgtaacc agtcaatcag cactagatgt
     2641 ggaatcagta gctgcggtga gtcgacgacc accgcctcgc gccacgcgct taccggtcag
     2701 tcgacgctac agggggccga ttgcagcgcg ctcaatcgtg cgaaggcaaa cgtatccgag
     2761 ctgaggcgct tgcccgcgcg ctccagttcc gagcgccctg cgttcgcctg gctacccacc
     2821 cgcgagctcg cgcagggtca tgtgacccgc ggtccaccgt cgtttcgcgt ttgcgcgtca
     2881 cagtcgcgtc gccaagggtg ctttcgttgc gtgagcctgc ctcggggctc gcgcctcgct
     2941 tcgcgagcta ggctgcacaa atccaaaagc agagaaacat gaccggttcg aagcgtgagc
     3001 cgtaactgaa gggtactcaa ccgcagcgcc tgaaagggcg ctatcaggag catctgcatg
     3061 agtcgttcga gttctcgcgc ccccctcttg tctttcgtgt ttctcgcgtt gggatgtgga
     3121 gataccggct ccgtcacgcc cgcgccttgg ggtggcggtg cgggaggcgt tggcgccgcg
     3181 ggtagtggcg ggagtttgtc caccggtgca gggttcggag ggttttccac gggccccgcc
     3241 gggggtgctg gcacctcgag ttcgggtggc gcgggttcgg gagcacaaaa ctccggcggt
     3301 gcgccgggac cgagttcacc gagctcgggt gggacttcga atcgcggagg tgtcccgagc
     3361 accaacccgg gtacgagcgg ttcggttaca ggcggcttgg gcggcaacgg aaactcgagc
     3421 ggcgctactg gccttggagg gattgccggc actggtgggt ctgcgagcgg agggcagggc
     3481 actgccggct cgaacgcagt tacgggtggt ggtccgaatg gcggcgccgg gacctgtgac
     3541 ccgggcagca cggctactca atgggcgacg aattgtccca cgtccccgcc cgcctgcacg
     3601 gccggtacct ggatcgcggg cgggcccgat cccgatcatt cgaactataa gttgatcaag
     3661 gagtcggagc acttcgccat ctattcggac gagagtgtat ccgacgcgaa cgccacgtcg
     3721 gcactcgaca ctctggagag caagatctgg agtgtgtact tcggcaatcc aatttacttc
     3781 aaagagccac tctgcaatca aaccaccaag tacaaagcga gcgttcacgt tcactcgacc
     3841 aacggcctga ccgggggcgc ttgggccgcg aaccggatgg gcatgtggat cggccccggc
     3901 gcgctcaccg accactgggg actcgcgcac gagttcatgc acggcgtgca gagcgtgagt
     3961 gggggaatga gttgcggcag cggtcccaat tactgcggtt ggatctacga gagccacgcg
     4021 aacttcatgc cccatcaaat cgcggagtat cgcggggacg tccattgttc cgagatgagc
     4081 ttcaatatgc cgcatctcta tctagggtcg acgcgggatc gatattgcaa ctggcaattc
     4141 atggagtact tgaaggacaa gtactgctat tcggccgtca acgcgatctg gacccagtcc
     4201 gcggccaacg atcctttcac caacatcatg aaggggatgg ggtggaacat ctcgcagctc
     4261 aacgatttca tcggcgagtg ggcgacgcac aacgtgacct gggactacaa gaatcccgct
     4321 cccacgctgg cgggggatac cctcgatccg ggcgccaact tccggagcaa gtacggtctg
     4381 atcacggata cttctcagac catccgacgt ctgcgcacga ccaagctcga tccattggac
     4441 gcgaactggg cgatgacacg acgtttcgct agcccgttct attgggcccc gcagcgcttc
     4501 ggatacaacg tcgtccgcct ctatccagac gccggtgcca cgacggtgcg cgtctctttc
     4561 cgcggtgtca ccacgggtgc gcccaatccc gactggcggt ggggcctcgt ggcaacgaac
     4621 tccacactca cgaccgcacg ctacagcgct ctgcagaagg gcgcggatgg agagttgtcc
     4681 ttctgtgtca gccctgggga gtccatatgg ctcgtggtcg cggcgacgcc gtccgtccag
     4741 caacaaatca aatgggacca gccttacggc accgttcctc gctatcctta tctcgtcgcg
     4801 ttcgggaatg cgtggccgga cggctttcag gatggaaagc aggcggcgtg ccccaacgga
     4861 ctcgtccggg tggcgaacgg cggtggatgc gggcccgcca acttgccagc atccgtttac
     4921 gttgggccct acgccactgt cctgagtggc gccacggtga gtggggaggc ccgcatcgaa
     4981 gatcacgcgg tcgtggcgcg cggcaccgtg tccggcggaa ccgtgggcgc catgactctc
     5041 atcgataact tcaagatgag cagcggcacg gccaaatcga cgttctatcc cctcggtttc
     5101 ttcgagaccg ggcaaggctt gtcggggggc accctgatcg gagacgtgga gtaccgaggc
     5161 gcggggttga gccgcacgtc cggtacgtgc agtggcttcg tagacgatgg cacctgcatc
     5221 gcaccgggcg ccgatacgac cgcgacgccg ccctttacct ggcgctagcg ctacagggcc
     5281 gttacacggc ccgcccgaga agaaggaacg acggggccca gcgctactgt cagtacccgt
     5341 tactgacagt agcgcattcg cacgatgctc aacagccgtt tggtaggcac gtcgggcaca
     5401 ccgtctcggt cggaacgtag ggggccagcg taaccgcgcg cgtcttctcg cacgaacgcc
     5461 caacccaaat ctccattggg gtatacgacg atctcgtctt ccgcgttggg cggcaccgcg
     5521 atcacttcgt cgtccgaaac tgggttgccc gcgagatcga tcaagcgcag gtgagtcccg
     5581 gtatacgtgc cgaagcacgt ctggtcgttg atccaccgca gctcctcgac gctatcccaa
     5641 accatcagca gtttgtcgga tccgtagcgc gcgaagtgca ggttcttctc cgcgaccgtc
     5701 ggtgtctcac tgagccgcgt agtcgggata cgcgggcggt agtccgggcc gagcagctga
     5761 aacacgatat ctgccggagt ctgtcctgga ttctctcctc gcgagagcca ccccacgaag
     5821 tagctgccgt cgcccagctt caccacggat ccgaactctc cccccgaata ggctcccgta
     5881 gtgtactctt cggcgagtgg aaccgaggcg cgacccccga tgactaggtt caacccgggc
     5941 gccgggaagg tcacggccat gcacagcgaa gtaaacgcac ctgcttcggg cagtagccgc
     6001 actccttcgt tgatgctgca gttccaggcc caaccgccgg gaacgaagcc gccctggtca
     6061 tccacatagc tcagcttgtc cgcatagtag cgctcgcgag ggtgcccctc acagccgtgc
     6121 accgtgaagt acgccccata cttctggcca ttgtaaacca gacggcttag aaacggccgc
     6181 ggcgcgcagt cgcgcttttc ggcctccgag aggttcgggt cgagttgcgc agttcccgtg
     6241 agcggcgccg cgaactcctc ttgcccattt cgatatctga gcagcaccgc cgcggtcgcg
     6301 agcgtgggcg tcgtgggatt gaggtctcgt agaggctcgc cgggatcgtc gcgccgggtc
     6361 agcaacgcaa agccgtcact gtgcaacact agccctccga tatcgcgcgc gggcacgatg
     6421 ctgtcctcgt cacgccgatt tcccgcggcc gtgagcggag tcacgtgcac tcgggtcagc
     6481 tcattttcga gccaggccac ctgagcgagc ccgtcaggac cgaccgccaa agccaaccgc
     6541 tcgtcttgcg ggattaggtc gtaagcaggg cgcttgtatc gaatgtcgtg atctaagggc
     6601 acgtcggtta cgctcacccg gccgagcagc gtttccggac aagaacccat ggccccaccg
     6661 gacggagtgc tacccccttg ggcgctcgcg ccagcgttcc ctgacgaaga cgcgcccgag
     6721 gcgctcgtgg ggtcgaccgt ttgcgcgtac tgcgggctgc aactcagaca gcacagcccg
     6781 cacgaaaacc tcagaccgca aaccaagagc gaatcacgtg ccggcgtcac agcccaatca
     6841 ccacacccgc ggtggagctc agcagcggtt gttgcagctt cacccgttcg tccaagacgg
     6901 cgatcccttg tctcggcatc aaagccagag cccgtagcga gaagcccagt gcaagccgcg
     6961 agctgagttg gagctgcgca tgcgcactga ttagccccac gaaaccccac gtcgaatccg
     7021 agcgcgaacg caggggcggt tcggcctgac cttcggcttg caggaagtgg ccacccaagc
     7081 ccgctccgag tccagcgtga aagtcccgag tccccatcaa ccagccgcgc gcttccaacc
     7141 aagccatctc ttgtcgagaa gaggcgccgc cttgggaagc ctcgaaatct gcgcccacca
     7201 gcggccccgc cgcgagcaac cagagctcga aatcagcgaa gcagcgggcg acgcccaatg
     7261 cgggtccgta gctggctccg agcgtcggac cattgagtaa ccccacgccc tgaatcgcga
     7321 ccgcataact tcgcagctcg gacgtcgcga actgccgcac ggcaggggga ggaggacgac
     7381 gatccacctc cgccacttcg ggaggcggct cccccacgga ttgaaactcc tcgaagctcg
     7441 cacgcagcaa atccacggcg cgaatcgcga gcacgctcga agcgtccgga gtgcggccga
     7501 gctcgatccg gcggagagta gttttaccgc tgatgcgatc gacgagccag atttccactg
     7561 ccgtcgagct gagcggctca cgatgcacga gcgcgatccc ggcgattgcc ccgaccttgc
     7621 gggcgatctc ggtgagcgag tccgccgtgt gctcaccgag cggcgcgctc accacctccg
     7681 tctcgaaatg ctggagctcc aactcggcgc gcagccgatt gcaggcgtcg agtaacaagg
     7741 tgtcgctcgc cccggacggg ctctccggtc gtactagcgc caccttttgc gaggcgggcg
     7801 cgcgctcggg cgtggccgca cctgccgctt ccgcgaacga aactaggccg cagatcccga
     7861 atacgatcgc gaggaatcgc atcctcagtc gagaccagcc agctttctcg ccgcaggcgc
     7921 ctgcactcct tcagggtaca gccgtaagta ctcgagcgcg agaggcttcg cgctcgccgc
     7981 gcctcgcacc tggtacgctg cacgcatgcg tcccgctagc gcgt
//
LOCUS       DASTKU010000168         7911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_32051,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000168 DASTKU010000000
VERSION     DASTKU010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32051"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /locus_tag="VFQ61_06000"
     CDS             complement(<1..1548)
                     /locus_tag="VFQ61_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9954033.1"
                     /translation="MDSNGDGIGDLPGITARLSYLAWLGVDAVWLSPIYPSPMADFGY
                     DISDFCDVDPRFGTLRDFEALIARAKELGLRVILDFVPNHTSDRHPWFVEARSSRDNP
                     KREYYIFRDGRHSPSDEAGALRQSSPIPPNNWLSEFGGSAWQWDARTEQFYYHAYLKE
                     QPDLNWRNPEVRAQMLRVLEFWLERGVDGFRVDTIHHLFKDELLRDNPPNPDFAPGMP
                     PHRRLIREFTTDRPEVQQAIAAIRQVVDRYPDRVLIGEAWLPVDRLMAYYGVDLSGLH
                     LPFNFQLLQARWEVPILRSIVARYEESLPSGAWPNWVLGNHDRPRIASRVGDAQARVA
                     AMLLLTLRGTPTLYYGDELGMKNVAIAPEQVRDPWEKNVPGIGVGRDPERTPMHWSGE
                     SGAGFTSGTPWLPLGADYVERNVDTQSKDPHSTLNLYRQLLTLRRREPALSIGSYREI
                     DTGPDVLGYVREYAGRRIAVLLNLSSHPQLPVLNPEVERAKLVLSTRAERELGSTPSE
                     VALGPDEG"
     gene            complement(1745..2275)
                     /locus_tag="VFQ61_06005"
     CDS             complement(1745..2275)
                     /locus_tag="VFQ61_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954034.1"
                     /translation="MARVFKNTICALAAACALFGTVGTARAVEPVTVTRAPAAEPSRV
                     RRPDRALLSSGLWTLGLSYVPAFVVAVESPRSFDNHLYIPVAGPWLDYGKRSCVDCKN
                     ETVNKVLLVTDGVFQGIGALQILGAFLFPETHTSTTTDAAKDKPRRTAGIASVRVVPS
                     RFETGAYGLTAKARFF"
     gene            2685..3332
                     /locus_tag="VFQ61_06010"
     CDS             2685..3332
                     /locus_tag="VFQ61_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9954035.1"
                     /translation="MATRSPSELEPRPRHFSMIREFRLADVITLLNGFAGTGALLSFL
                     RFVADRDARFFWLGTALIPVALVMDVLDGRVARQRQEASPLGQELDSLADVVSFGVAP
                     AAMAFVAGLSGGLDALCLMYFVACGISRLARYNVTAPGLSAAHGKVTYFEGMPIPSSL
                     LLVALMAVLAWKGYWQEQVPLGAWELGPVTLHPLALLFVLHGSAMISKTLRIPKP"
     gene            complement(3347..4618)
                     /locus_tag="VFQ61_06015"
     CDS             complement(3347..4618)
                     /locus_tag="VFQ61_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9954036.1"
                     /translation="MTRRAGNECEERMTEERYRQSAAWSERAQRVIPGGHHLSGRLLL
                     EADDSPQYMTRARGARCWDVDGNEYIDYVGAYGPFLLGFANEAIDAAAFEQLRRSGLV
                     SLNHPLHVEFAERLVSRIPGAEMATFFKTGSEATTAALRIARAATGRRRVVRCGYHGW
                     HDWCLPLERFVPRGLDEQVLEIQARDADALETALSTAPGIAAVIIAPEMVLPYDPAVF
                     HRLVELTHRYGALFILDEVKTGLRIKPGTFQQFAGLTPDLTTLSKALGNGWPIAAVVG
                     KREIMRHAAGMHLSATFHGDTAAMAAALSTLDFVDRHPVAEVVWELGQRLIDGLNAAA
                     RRQGIPAVAFGEPLPPMPFLKFTASEPGLNDSLRSAFYREVYRRGVLLHPRHLWFVSY
                     AHTPADIDYTVQVAEEAFARAARALGARRTW"
     gene            complement(4590..6587)
                     /locus_tag="VFQ61_06020"
     CDS             complement(4590..6587)
                     /locus_tag="VFQ61_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET9954037.1"
                     /translation="MPRRHLSQIGLRASIPPVAGDDSDPTSPGNSEVMGYPRPQLRRR
                     EWFSLNGEWDFALDPEGQWTVPEQVAWSARITVPFSPETQRSGIGDTSLYRACWYRRR
                     FQAPALDGKQRLVLHFGAVDYQATVWVDGTLVARHEGGYTPFWVDITDALEADAVNLL
                     GAEAEGASADGTTSLREAASPGSPVEHEIVVLAADDPADLAKPRGKQDWQLDPHSIWY
                     PRTTGIWQTVWMERVNPVRLDRLHWTPNLERWEISLDTRIFGYIDEKLFLSVRLRVED
                     LILADDKYTVVAGEVHRRIALSDPGIDDFRNELLWSPERPKLIEAEIELLNAKGERLD
                     SVTSYTALRAFAVQGDRFLLNGRPYPLRLVLDQGYWDGAGLTAPSDAHLRRDVELARA
                     MGFNGVRKHQKIENPRYLYWADRLGLLVWEEMPSAYRFTRESVERMAREWLEVLRRDA
                     SHPCIMAWVPFNESWGVPNLPDSLPERNYVRSLYYLTKTVDPTRPVVGNDGWESVTTD
                     IIGIHDYDENPERLGRRYWAHSTLPKLLKRERPAGRSLVLEGHPRREHPIVLSEFGGI
                     AFSDEPGSWGYSRSESAAAFAEKFSALLRTTRSLEVLAGYCYTQFADTYQEANGLLRS
                     DRTPKIPLEQIALATRGSGRADDVLPPVTLSEDDPTRGQRV"
     gene            7093..>7911
                     /locus_tag="VFQ61_06025"
     CDS             7093..>7911
                     /locus_tag="VFQ61_06025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9954038.1"
                     /translation="MFFFEEPIFDDGPNRLELQQSPEGVHVATPHLTHGCSDVDGEQR
                     RLLQELLTREQVEDYALWYYTPMALPLTQGLTPRIVVYDCMDQLSAFKNAPPALTERE
                     RELFSLADVVFTGGQALYESKKHQHRNVHAFPSSVDVAHFAKARVPQTEPADQASIPG
                     PRLGFFGVVDERMDLELLAGVAAAQPDWHLVILGPVVKIDPAELPRAANIHYLGGKDY
                     SQLPSYIAGWDVALLPFARNESTEFISPTKTPEYLAAGKPVVSTTIRDVVRPYQK"
BASE COUNT         1517 a         2469 c         2398 g         1527 t
ORIGIN      
        1 gccctcgtcg ggccccaatg ccacttccga tggcgtggag cccagctcgc gctctgcacg
       61 ggtcgaaagc accagcttgg cgcgttcgac ctcagggttc aataccggga gctgcggatg
      121 cgatgagaga ttgagcagga ccgcgatgcg ccggcctgcg tattcgcgca cgtagccgag
      181 aacgtcgggg cccgtgtcaa tctcgcgata ggagccgata ctcaaagccg gctctcgcct
      241 gcgtagcgtg aggagctgtc gatagagatt gagcgtggag tgcgggtcct tgctctgcgt
      301 atcgacgttg cgctcgacgt agtcagctcc gagtggcaac catggagtgc cagacgtgaa
      361 gccggcgccg gactctcccg accagtgcat gggcgtgcgc tccggatctc gcccgacccc
      421 gatcccaggt acgttttttt cccatggatc gcgcacttgc tcgggtgcaa tggcaacgtt
      481 cttcatcccg agttcatcgc cgtaatagag cgtgggggtt ccgcgcaggg tcaacaacaa
      541 catggcggcc acgcgcgctt gggcgtcccc tactcggctg gcaatgcgag ggcggtcatg
      601 attaccaagc acccaattgg gccacgctcc cgaaggaaga ctctcctcgt accgggccac
      661 gatgctgcgt agaatcggga cttcccagcg ggcctgcaat agctggaagt tgaacggcaa
      721 gtggagcccc gacagatcga ctccgtaata cgccatcaaa cgatcgacgg gtaaccacgc
      781 ctctccgatc aggacccgat ctggatagcg gtctaccacc tgacggattg ccgcaatcgc
      841 ttgctggacc tcggggcggt cggtcgtaaa ttctcgaatc agacgccggt gcggcggcat
      901 cccgggggcg aagtctgggt tggggggatt gtctctcagc aactcgtctt tgaacaggtg
      961 atgaatggtg tcgacgcgaa atccgtccac gccgcgctcg agccagaatt cgagcacacg
     1021 caacatctga gcccgaacct ccggattgcg ccagttgagg tctggctgtt ccttgaggta
     1081 cgcgtgataa tagaactgtt cggttcgtgc atcccactgc catgccgaac ccccgaactc
     1141 gctcaaccaa ttgttgggcg gaatggggga ggactgtcgc aacgcgcccg cttcgtcact
     1201 cggagaatgc cgtccgtcgc gaaatatgta atattcacgc ttcggattgt ctcgcgagct
     1261 ccgcgcctcg acgaaccaag ggtgccgatc ggaggtgtga ttgggaacga agtccaggat
     1321 cacccgtaaa ccgagctcct tcgcgcgtgc gatcaacgcc tcgaaatctc gcaacgttcc
     1381 aaaccgcgga tcgacgtcgc agaaatcgga gatgtcgtag ccgaagtcgg ccatcggaga
     1441 cggatatatc ggcgatagcc agaccgcatc tactccaagc caggctagat aggagagccg
     1501 ggccgtgatc cctggtagat cgccaatccc gtcaccgttg gagtccatga acgaccacgg
     1561 gtacacctgg tacacgcaac cgctctgcca ccatggatgc catctcggat ctcgagtcct
     1621 ggacgtcatg ccataggcta gaccatcccc tcaccgaggg cccggaagaa gcacggcgcg
     1681 cgcgcgaata tcggagtcgc gttcgaagag aactaccata actgggccgc ctacatcgct
     1741 ctcgtcaaaa gaaccgggcc ttcgcggtca gcccgtacgc acccgtttcg aagcgcgaag
     1801 ggacgacgcg caccgaagcg atgccggcgg tgcgtctcgg cttatccttc gccgcatcgg
     1861 tagtggtgga cgtgtgtgtc tccgggaaca agaatgcgcc caggatctgt aaggcgccga
     1921 tgccttggaa tacgccatcg gtgacgagca atactttgtt cacggtctcg ttcttacagt
     1981 ccacacacga tcttttgcca tagtccagcc acgggcctgc caccggaatg tacaagtgat
     2041 tatcgaagga gcgcgggctc tctactgcca cgacgaacgc cggaacatag ctcaaaccga
     2101 gggtccaaag gccagagctc aacaaggctc ggtccggcct tctcactcgg ctgggctcgg
     2161 ccgcaggggc gcgcgtgacg gtgaccggct ccaccgcacg agcggttccg accgttccga
     2221 acaacgcaca agccgctgcc agagcacaga tggtgttttt gaagacgcga gccatgggaa
     2281 ctaacctctt ttttgcgagc cattcggctc tgcgtgggcc ctgctctgtg tctgtagcca
     2341 cgccctccgt aactgctgtc tcggaagctg ctggttcggt tgccaacagt tgagctcctg
     2401 cgactcgtgc tactctgcgg gcacagccgt tcgcggtatg aatcgagctc gttgcggtgc
     2461 gtttcgagtg ggttgccacg actgaaacca ggtatcgcaa gagcaatgcc agagcggggt
     2521 gtgaccctcc gttccccaca tcctcgaggg gtcgcgcact tacgaagcca aaagaggact
     2581 ggtctgtcac tgcgaggcgt gcctgcgtgg gcttctccgc gatgcacgct cacacgcagt
     2641 tgtggcttgg gtacggcggg tttctcggtg taagttccgc caaaatggcc acgagaagcc
     2701 catccgagct ggagccgcgc ccgcgtcatt tttccatgat ccgggagttc cgcctggcag
     2761 acgtcatcac tctgctgaat gggttcgcag ggacaggtgc gctgttgtcg tttttgcgct
     2821 tcgtggcgga tcgcgacgcc cggttcttct ggctgggaac ggcgctgatt cccgtagccc
     2881 tcgtgatgga tgtactcgac ggccgtgttg cccgacagcg gcaagaggcc tcgcccctcg
     2941 gccaggagct cgactcgctg gcagacgttg tctccttcgg ggtcgcgccg gctgcgatgg
     3001 cattcgtggc ggggctaagc ggcggactgg acgcgctgtg tttgatgtac ttcgtggcgt
     3061 gcgggatcag ccgattggcg cgctacaacg tcactgcgcc ggggctctct gcggcccacg
     3121 gcaaagtcac ttacttcgag ggtatgccaa tcccgagcag tctactactg gtggctctga
     3181 tggcggtctt ggcttggaag ggctactggc aagaacaggt gccgctcggc gcatgggagt
     3241 tgggccccgt cacgctgcac ccactcgcgc tgctgttcgt gctgcatggc tcggccatga
     3301 ttagcaagac cctccgcatt ccaaagccat gaaacagggg cgcggttcac cacgtgcgcc
     3361 gtgcgccgag cgcccgagcg gctcgcgcga acgcctcttc cgcgacctga accgtgtaat
     3421 cgatgtctgc cggagtatgc gcgtagctca cgaaccatag gtgacgcggg tgaagtagaa
     3481 cgcctcgccg atacacttca cgatagaacg cggagcgcag cgagtcgttc aagcccggtt
     3541 cgctcgcggt gaacttcaaa aacggcatcg gtggcagggg ctctccgaaa gccaccgccg
     3601 ggatcccctg gcgtcgagcg gcggcgttca agccgtcgat caggcgctgc ccaagttccc
     3661 aaaccacctc cgctaccgga tgacgatcga cgaagtccaa tgtcgaaagc gcggcggcca
     3721 tggccgcggt gtcaccgtga aacgtcgcag acaagtgcat tccggcggcg tgccgcataa
     3781 tttcacgctt gcccaccacc gccgcgatag gccagccgtt gccgagcgcc ttactcaacg
     3841 tggtcaaatc tggagtaagc cctgcgaact gttggaacgt accaggcttg atgcgaagcc
     3901 cggtcttcac ctcgtcgaga atgaatagcg cgccgtagcg gtgggtcagc tcgacgagac
     3961 ggtgaaagac ggccgggtcg tagggcagaa ccatttcggg agcaatgatg acggccgcga
     4021 tacccggggc cgtacttagc gcagtctcca gagcatccgc atcacgcgct tggatctcca
     4081 gtacctgctc gtctagacct cgtggcacga agcgctccaa cggcaaacac cagtcatgcc
     4141 agccgtggta accgcaacgc accacacgcc gacgccccgt ggccgcccgg gctatgcgca
     4201 aagccgcagt ggtagcctcg gatcccgttt tgaagaaggt cgccatttcg gcgccgggaa
     4261 tgcgcgagac caagcgttcc gcaaattcga cgtgcagcgg gtgattgagt gagaccagcc
     4321 cacttcgtcg gagctgctcg aacgcggcgg cgtcgatcgc ttcgttcgcg aagccgagta
     4381 agaagggccc atacgcaccc acgtagtcga tgtactcgtt gccatccacg tcccaacagc
     4441 gcgcgccgcg ggcacgcgtc atgtattgcg gcgaatcatc ggcctcgagc agcagacgcc
     4501 ccgagaggtg atgcccacca gggatgactc tttgagcacg ctcggaccac gcggctgatt
     4561 gacgatatcg ctcctcggtc atccgctctt cacactcgtt gcccgcgcgt cgggtcatcc
     4621 tcactcaaag tcaccggcgg tagcacgtcg tcggcgcgcc ccgagccacg cgtcgcgagt
     4681 gcgatttgct cgagcggaat cttcggtgtc cgatcgctac gcaataagcc attggcctct
     4741 tgataagtgt cggcgaactg ggtgtaacag taccccgcca gcacctccag agatcgcgtg
     4801 gtgcgaagga gcgcgctgaa cttttcggca aaagcggcgg cgctctcgct tcgtgaataa
     4861 ccccaagagc caggctcatc gctgaaggcg ataccgccga actccgaaag cacgatcggg
     4921 tgttctcgac gtgggtgccc ttccaggact aggctgcgtc ccgcggggcg ctcgcgcttc
     4981 agcagtttgg ggagtgtgct gtgagcccag tagcgtcgac cgaggcgctc tgggttttcg
     5041 tcgtagtcgt ggataccgat gatgtcggtg gtaacactct cccaaccatc gttgccgacg
     5101 actggtcgcg tcggatcgac ggtcttggtg agatagtaga gcgaccgcac gtaattgcgc
     5161 tccggtaagc tgtcgggcag attgggcacc ccccaggact cgttgaaagg gacccaggcc
     5221 atgatgcagg ggtgactcgc gtccctacgc aaaacctcca gccattcacg ggccatccgc
     5281 tcgacggact cgcgcgtgaa gcgataggcg ctgggcattt cttcccacac caacaagccg
     5341 agccgatccg cccaatatag atatctggga ttctcgatct tttggtgttt ccgcacgcca
     5401 ttgaagccca tggcccgcgc cagctctacg tcacgtctca gatgcgcgtc gctcggagca
     5461 gtgagtccgg ctccgtccca atacccctga tccaaaacca agcgtagcgg atagggccgc
     5521 ccattgagta ggaaacgatc tccctggacc gcgaaggcac gcagcgccgt atagctggtg
     5581 accgaatcca ggcgctcccc tttggcgttc agcaactcga tctctgcttc gatcagtttg
     5641 ggtcgctcgg ggctccacaa cagctcgttg cggaagtcgt cgatgccagg gtctgaaagg
     5701 gcgatgcggc gatgcacctc accggccact accgtgtact tgtcgtcggc caagatcaga
     5761 tcttcgacgc gcagacgcac cgacagaaag agcttctcgt cgatgtagcc gaagattctc
     5821 gtgtcgagcg agatctccca gcgctccaag ttgggcgtcc agtggagccg atcgagccgc
     5881 accgggttca cccgttccat ccataccgtc tgccaaatgc cggtggtgcg cggataccaa
     5941 atcgagtgtg gatccaactg ccaatcttgt ttgccgcgag gtttggcgag atcggccgga
     6001 tcgtcggccg cgagcacgac gatctcgtgc tccaccgggc tgcccggtga ggccgcctct
     6061 cgcaatgacg tagtaccgtc agcgctagct ccttctgcct ccgccccgag caggttcacc
     6121 gcgtcggcct ctagagcgtc ggtgatgtcc acccaaaagg gcgtgtaccc gccctcgtgt
     6181 cgcgcaacca aggtaccgtc gacccagacc gttgcctgat agtcgacggc tccgaagtgc
     6241 aggaccaagc gctgtttgcc atcgagagct ggcgcttgga agcgccgccg gtaccaacac
     6301 gcgcgataca acgacgtgtc gccgatcccg ctccgctggg tctccggcga aaacggcacc
     6361 gtgattctgg cggaccacgc cacctgttct ggcaccgtcc actgaccctc cggatcgagc
     6421 gcgaaatccc actccccgtt caaactgaac cattcgcgtc ttcgcagctg cgggcgagga
     6481 tagcccatca cctcgctgtt ccccgggctg gtcggatcgg agtcgtcacc ggcaaccggg
     6541 ggaatggatg cacgtaggcc tatttgagag aggtgccgtc ggggcatgga cactcagagc
     6601 tagtttcgag gcttggaacc cgcacggccg aactggacgg ctggtgggta ttggcgcgcg
     6661 actcgattcg atgattatca ccgaaattgc gcgctcattt gtgcacgaga cataccagaa
     6721 ggcctcgcga acgaggattc ctagaatcac gtttgaaaac gaggtgacga atcccgtggc
     6781 aaaaccggag cgcctcagat gggcaatttt gggtcggcgc tcttgctagt atgtcgttcg
     6841 taagggcgga ttccgcatga tttcattggc atgggaactg cttattgcag tcaataaccc
     6901 tcattaggcc ctacccgcgc acggcgccct gagtgcccct gccgtggtgc cgtggccgtg
     6961 ccctgcgcga gcgcaggcag agcgagcatc actatgcaca attatgacgt agtctgcctt
     7021 tcgcatcttc gctggtcctt cgtctttcaa cgaccgcagc atttactgag ccgctgcgct
     7081 cgcgaacgcc gagtgttctt tttcgaagaa ccgatcttcg atgatggccc gaatcgactc
     7141 gagctgcagc agagtccgga aggggtccat gtagcgactc ctcatctcac gcacggctgc
     7201 tcggatgtcg acggcgagca acgtcggctg ctgcaagagc tgttgactcg cgagcaagtc
     7261 gaggactacg cgctctggta ctacacgccc atggcgctgc cgctcacgca aggactcacg
     7321 ccgcgaatcg tggtgtatga ctgcatggat cagctctccg cgttcaagaa tgctcccccc
     7381 gcgctgaccg agcgcgagcg cgagctgttc tcgctggccg acgtggtgtt caccggcggg
     7441 caggcgctgt acgagtcgaa gaagcaccag caccgcaacg ttcacgcatt cccgagcagc
     7501 gtcgatgtgg ctcacttcgc caaggctcga gtgccgcaaa ccgagcccgc ggaccaagcg
     7561 agcatccctg ggcctcgctt gggcttcttc ggcgtagtcg atgagcgcat ggatctcgag
     7621 ctcctggcgg gagtcgccgc ggctcaaccc gattggcact tggtgattct gggtccggtg
     7681 gtcaagattg atcccgcgga gttgccacgc gccgccaaca ttcactatct gggcggcaaa
     7741 gactactcgc agctgccgtc gtatatcgcg ggctgggacg tcgcgttgct cccgtttgca
     7801 cgcaacgaat ccaccgagtt cattagcccg accaagacac ccgagtactt ggccgctggc
     7861 aagcccgtgg tttccacgac gattcgtgac gtggttcgcc cgtatcaaaa g
//
LOCUS       DASTKU010000169        22754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_700474, whole genome shotgun sequence.
ACCESSION   DASTKU010000169 DASTKU010000000
VERSION     DASTKU010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 22754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22754
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700474"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VFQ61_06030"
     CDS             complement(<1..313)
                     /locus_tag="VFQ61_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954039.1"
                     /translation="MTDCYPTRAALGTRTLNGIRTVSAPPSRSRALEWRAAKSFLLTF
                     SAALVAVACQQTPGGVYVDELQIDHGVDRGGTSGRTTDPQGEPSRGGDGSGGTRSGTA
                     PG"
     gene            1083..2147
                     /locus_tag="VFQ61_06035"
     CDS             1083..2147
                     /locus_tag="VFQ61_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954040.1"
                     /translation="MIDQKSLILAGQDTSRLQAAPAAGAASLAVATLMAACIVGCGPA
                     EPSANDIESLEQSATLSAVAKVADTTRVTLAAAELARVVPTSPAKDCSMFDAYTAALA
                     TSVIDCLGTIGPDVFAVDEKGYLNRRFDKCLENSEEQLANIDGLLSLQVREHQRPNKS
                     GIRLDPVACISGRYQRWAGSFKAAGIRECPRWVKTGVIGETTPEVVATYTKLLPQIST
                     KGTRVERDPPVAKRDYAYKVYFASGIQDKGCQDPASCAAACVSGFPGALVKASGEEVL
                     LDPTYWLDATKTTVYTAGGYYHGMSYYGGPPGDLFGHINRQNETCSYYDFDFDMHYLG
                     KLKPVQIGGMFASWMTECMP"
     gene            2257..3318
                     /locus_tag="VFQ61_06040"
     CDS             2257..3318
                     /locus_tag="VFQ61_06040"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HET9954041.1"
                     /translation="MSLLIYGANGYTGELIARRARAIGVDPERVILAGRNAGSIAALG
                     QSLGFGTRVFGLDDPARLDAVLDGVQVVLNCAGPFIRTALPLVEACLRRRAHYLDISG
                     ELAVLEGLAGRDQQARAAGITLLPAAGFDVVPSDCLAAEIHARLPAAVELTLAFEAPE
                     GMSRGTAITSLEQSPRGGVVRRDGKLVRVPTGHRTRQIDFGSGERTAICIPWGDVATA
                     YYTTGIPNIEVYAALPWPMRAAIRASNWLRPVLSSKGFQRLVVDQLMAGATGPSDTQR
                     ARGQSLLWAEGRDDEGRSVQLRLRTPESYELTSWTALELAERARHGKLPVGFQTPARA
                     CGKGLIQQFPGVSLTEVLV"
     gene            3477..4865
                     /locus_tag="VFQ61_06045"
     CDS             3477..4865
                     /locus_tag="VFQ61_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954042.1"
                     /translation="MQDQLESPLQRVIAHIRDKRFTGLLRVRAQEAEGELWFLSGIRE
                     DVKFGLSKGDEALDRMLRATQPSFEALPRLPGLNAGFKKALALTGTFTEAAPVVLMQH
                     CETQALTCVLELVAANHVVRAKYQVGELLSLDVDSGGDAAVTETLNAAEGTYRFTLPD
                     FELPDGVALTAPSPAERPDIRVIGVPATDGEQAAARKAEAEAEARRRAEAEAEARRKA
                     EAEAEARRKAEAEARRKAEAEAEARRKAEAEAEARRKVEAEARRKAEAEAEARRKAEA
                     EARRKAEAEAEARRRVEAEARRRAEAEAEARRKAEAEAEARRKAEAEAEARRKAEAEE
                     EARRKAEAEAEEARRKAELEAEEARRKAEAEAEEARKKAEAEEEARKKAEAEQERREA
                     EERRRVEERKRREAEEAEEEAALAERPSRRVLRTGSTPPVPKSGPGPLMILLLLVLAA
                     AIAYVVVGLLRR"
     gene            5665..7827
                     /locus_tag="VFQ61_06050"
     CDS             5665..7827
                     /locus_tag="VFQ61_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HET9954043.1"
                     /translation="MTGSSVRKQAIAEIASQKQVASAPSKESVDALFACNVFGLAAMK
                     THLPKDVFRSLKRTIEKGEPLNPVHADVVASAMKDWAVARGATHYAHLFYPLTGAMAE
                     KHDAFLSPDGEGGALGEFSGKALIQGEPDASSFPNGGIRATAEARGYTAWDVTSSAFV
                     IDSTLYIPTAFVSWTGEALDKKTPLLRSMQALNRQATRILKLFGHENPAPVVAYAGPE
                     QEYFLVDRRFFLARPDLMNSGRTLLGAKPPRGQEFEDHYFGAIPERVLAFMVEVEREL
                     FKLGIPVKTRHNEVAPAQFEIAPVFESANIAADHQQMTMMTLRRIAVRHGFECLLHEK
                     PFAGVNGSGKHVNFSFGNSTQGNLLDPGDTPHENAQFLVFCAGVLRAVHRHSGLLRAS
                     VASASNDHRLGANEAPPAIISVFLGEQLADVFEQIKAGGAKSSRSKGTLEIGVDTLPK
                     LPKDAGDRNRTSPFAFTGNRFEFRAVGGSQSISGPLVAVNTAVAEALDTVASKLEAAV
                     ASGKSLNAAIQGVLTELANEHAAIIFNGNGYSEEWHKEAERRGLPNYRNTVDALPVLE
                     QPEVVRMFDKYAVLTPRELKSRYEIYVEQYIKTVQVEAKLVGRIAKTQVLPAALRYQA
                     ELAQGLAAAKSAGLTISSKLAEDVSRNIAELEKGLSALDTAMSHHGASDALAEARQLS
                     KDVLSAMSAVRTAADALEVSVADDLWPLPTYQEMLFIR"
     gene            complement(7992..8804)
                     /locus_tag="VFQ61_06055"
     CDS             complement(7992..8804)
                     /locus_tag="VFQ61_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954044.1"
                     /translation="MSDDDELVAVSPRHSVRLANEYAILLKRPPRHDFVVRTIMRREA
                     NGLPLVSADIVIAGSDTRAEFPIAAQYPLHFRKTYFPGRLHGDPRQEFDRQAEASAIL
                     DLPPPIGCSNDVFRSCFLPGTPYHRLSPFGVEPEESNIPLARKLSVATAAGLWGFVDG
                     AFRALQQLHEKGLTHGDAELHNFVVCPAPLEILVVDFEASERRADMPPDKWAARCSAD
                     FLPILREAILIQCCLGRQPGGLGEMSIARIHELFKNPDRFRREVGEKTSPQA"
     gene            complement(9119..9739)
                     /locus_tag="VFQ61_06060"
     CDS             complement(9119..9739)
                     /locus_tag="VFQ61_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9954045.1"
                     /translation="MSKAARSHRFAASPERLSQQSTFRLSVLANLFRDHRLSLQTAAF
                     KLTQDRAEASDLMQDAAERALRCAPLVSEEDLLRWVCAVMRNLAVDRFRQQRARSRAL
                     VSLRVLEWMQLQSSQLPDAETTPEADWVEFSAEDIARATQQLSAPFRRVFELSRELPL
                     THVAAQLGLPPATVGTRLFRARRKLRELLRRNSSSESPGSAPRRCA"
     gene            complement(10019..11362)
                     /locus_tag="VFQ61_06065"
     CDS             complement(10019..11362)
                     /locus_tag="VFQ61_06065"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9954046.1"
                     /translation="MKSRRIQERLNRRQLLRGLGGLTLGLPLLRSVQAKAEVPSPPKR
                     LVLMFAPNGVIPDAWWPQNARSETDFDLAACHQPLSPFKNRLTLFSGVDLRVAAEGPG
                     GLHQRGIGGLFTGQQLQTGTAFVDGCGQTSGWADGMSVDQAVAQQIGKDTLLTSLELG
                     LHATDNDVQGRISYAGPGQPLPPLNNPFDVYNRLFSMLGGGGDAVDALRANRRSVLDA
                     VSAQFGALSQQVSKEDQQKLDLHLTLVRDVERRLTDLGGGACSAPTPPPSLDPTLVEN
                     MPQIADLELDLLAIALACDLTRVASFAISSSLNRIGYPWLGSTSEGHTLSHAGVSDES
                     AKQQLVRRHTWHAERLAYLLTRLASYQEPDGTSVLDNTLVLWGSEVSLGYTHSHQNMP
                     FLMVGGGWHFRTGRYLTYQNAPHSNLLVSVMNAMGVPVTTFGRPELCTGPLSNLT"
     gene            complement(11359..13065)
                     /locus_tag="VFQ61_06070"
     CDS             complement(11359..13065)
                     /locus_tag="VFQ61_06070"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9954047.1"
                     /translation="MNRSTLVLIGVCAGLLSSSCAESSANNGSPGGASGAAFGGSSNG
                     AGNGGMAAGAGGVNACLNPPTAPLSLLTRMQYENTVRDLLGDGPSAAADFPPENQVEG
                     FKNNTQANQASPVLVEKYLEAAERLAGSAAQMASALAPCEAGREQVECGRDFVRNFGL
                     RAFRRPLLASETSIFDALFGRVQAQLGYTSAVELTLQAMLQSPQFLYRVDSRGAPSEA
                     SGAVALSSYELAARLSYFLTASTPDSQLLEAARAGRLSADEEIEAQARRLLETSRAKE
                     VVREFHHQWLGLDAIAALSPMAPELGEDAALLTKDWLGSLDRFIDTVYWDSGNLQTLF
                     NSKQVFVTPRLAKLYGAPLSGAQPSDSDFQAVTLSDRRGLLTQPALLGLLSHSDQTAP
                     VLRGVFTLKRLMCVAVPNPPPSVNNTPPDPDPNSTTRERFRVHTASTACSGCHRMIDG
                     VGFGFEAYDQLGRYRTEENGIPIDASGTVVIGDSVLDGDFDGVNELADRLETSDRVRD
                     CVATNWYRFAMGRMETEADKCSLDDIKAQFSASGGDLKQLLVSIPKTIAFRYRPAIGG
                     NP"
     gene            complement(13269..13787)
                     /locus_tag="VFQ61_06075"
     CDS             complement(13269..13787)
                     /locus_tag="VFQ61_06075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9954048.1"
                     /translation="MANPTATFSTSLGDFTVELLADKMPATTQNFIKLAKNGFYDGLH
                     FHRVINGFMIQFGCPFSRDPASPRAGTGNGPDGTIPDEHPENAKISNEPFTLSMANTG
                     RPNSGSCQFFINTVHNAYLDWFSPGPSKHPVFGRVTAGTDVVQKIERTPTDGRDRPTT
                     PVKMLKVTVHEA"
     gene            13992..14939
                     /gene="argF"
                     /locus_tag="VFQ61_06080"
     CDS             13992..14939
                     /gene="argF"
                     /locus_tag="VFQ61_06080"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET9954049.1"
                     /translation="MKDLLRTLDLSRQDFKYLLSRASKFKAHPHSRHKLLFGDSICMY
                     FAKPSTRTRISFEAAIARLGGNPLALNPADLQLGRGETLEDTARIISRYARALVIRTF
                     KDEDVHRLAKAATIPIVNALTDGHHPCQSVADMLTIQEHRGSLKNCKIAYFGDGNNVA
                     ISLMEASALVGADFVIACPEGYSIPEPLIEQARAVADETGGTIRVTRDPEEAARGADA
                     LYTDVWLSMGDADSEREARFNALSPYQVNERLSKLAAPGAIFLHCLPAHRGEEVTADV
                     IDGPSSMVWDQAENRMHTSMAILYGLLEGKIEGRQSERR"
     gene            15269..16228
                     /locus_tag="VFQ61_06085"
     CDS             15269..16228
                     /locus_tag="VFQ61_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954050.1"
                     /translation="MTRLVAVDSHVFQPRKQRLEGSEAILSHLKRHADSGNLVQGAAH
                     LMRAARSDEERSFEAVDGEWVPVQAAPADTHSEPNAKQRAALENVIAELRAELLMLRA
                     SHQRLRERVATLEARIENAPLPEKKSARAELATPARLSEARSARTEVKSATAAHKPAE
                     PLSELGLAPQSDRDLLGVLADLLGEDSGYAQIQRAIPESYLQRLGMYVSLLVDESGRE
                     VGAIVSHVACTAELGGRLAGLTPEERHQQARSGELSETVTQAMSEVCNALAGVLARSA
                     KSPGVRCTPLEAFDPDRLGWLGEARQRLCLSKPDGGAFWVLGR"
     gene            complement(16302..17264)
                     /locus_tag="VFQ61_06090"
     CDS             complement(16302..17264)
                     /locus_tag="VFQ61_06090"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HET9954051.1"
                     /translation="MEDVVSESADSAAPVPHPLDGWSAERITSAVRQDPKSLGPMSIG
                     RPNAGMLFNGEQAHASELYALVDPRHAFGTRETLVYLERALRKVHQAYPDSAPLVIGH
                     ISAERGGPLSPHVSHQAGRDVDISFFYREPKSWYTRATLQNLDVARTWAFVRALVTET
                     DVEMILIDHSIQAWLKEYALGQGEDAAWVESVFRGSAPSTPPLVRHAPGHATHIHIRF
                     YNPIAEETGRRAHAALRAAGVIPELTIYAKYRAKHGDTLKKISKRFSVSIESIQRANG
                     LKNSRIRENREYRIPLRGRTPLAPASARRVPARRLPPSTALSQR"
     gene            complement(17500..18444)
                     /locus_tag="VFQ61_06095"
     CDS             complement(17500..18444)
                     /locus_tag="VFQ61_06095"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9954052.1"
                     /translation="MRVLITGGAGFIGSHIVHGLVAEGHEVMVLDNLSSGRRENVPAG
                     AAFRQLDLRDREAVRQLVAEFRPEAVSHQAAQASVAVSVREPHLDAEVNILGGLNLLD
                     ACTAPGANVKRFVFASTGGAIYGEVPEGTQATEATGSNPQSPYAIHKLAFEQLMGVYA
                     KHRGLETRVLRYANVYGPRQDPHGEAGVIAIFFDAALSGKGIRINARRSVGDAGCVRD
                     YVFVSDVAQVNRLALYGQVTEPVVNLATGVPITTRELAETILKVTGRSVPLEPAPSRP
                     GDLERSVIQPTLALRYIERLTPLEEGLRQTHAFYASSR"
     gene            18522..19112
                     /gene="pdxH"
                     /locus_tag="VFQ61_06100"
     CDS             18522..19112
                     /gene="pdxH"
                     /locus_tag="VFQ61_06100"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET9954053.1"
                     /translation="MELEALSDPLAALLAWHREALEKNLPEPDACTLATATLSGKPSA
                     RIVLFKGIVNGDVQFVTNYASRKGREIAENPAVALVFYWHALLRQVRVEGEASFATAE
                     ESDAYFRTRPRESQLGAWASLQSQPVASRAELERRLREVEQRFEGKEVERPEHWGIYR
                     IRVETVELWVAGAHRLHDRCLFTRTAEGWRPARLFP"
     gene            complement(19177..19419)
                     /locus_tag="VFQ61_06105"
     CDS             complement(19177..19419)
                     /locus_tag="VFQ61_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954054.1"
                     /translation="MVTETLVDQRTFLWDFFGPAAPLTAAHFKKHLDEFLARNALEGC
                     ETGTRSEAPGHMAAFCRTPSVHWSAVQHSLRPKRAL"
     gene            complement(19433..20035)
                     /locus_tag="VFQ61_06110"
     CDS             complement(19433..20035)
                     /locus_tag="VFQ61_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HET9954055.1"
                     /translation="MALPSPIEVSQRRSFIGRLSPRRMGLYVLLLSLSMFFAASLVGY
                     FITRAQAAVFRVPEMTGAPRGLWLSSALLVAQSFTLQAALSAVRANRLGSLRSRLALG
                     FVLGVVFVLVQGENWYSLIRTERLLGVHTLYTFTFYLLTGLHAAHVIGGLIPLGWVWK
                     RARQAEYSSSHHEGISLCVQYWHFLSIVWAILFVALMFFP"
     gene            20274..22427
                     /locus_tag="VFQ61_06115"
     CDS             20274..22427
                     /locus_tag="VFQ61_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9954056.1"
                     /translation="MLLKPRQTNQLSKPRTALPLALVSGVFSAFCLVLADLVHRDNFD
                     YVPLGRHLFGALGMYTGVGVLFGVLLLGLVSLESRLIARRATPRARWARPFFYGIVGA
                     LASLSTAISVFSGERVQKTLLVYVGPIVFSGALGLAALIGSAFVLRILAAPPRAAVSG
                     ALGFLALGALIARVDLTQYVALYSRIHTLLEVIAALCFGAAMVIAARLARQSRPSLDV
                     TLAALSLLFGAWFVATLCVPALRRSFDEALRHVWLDEVYAGRMLRRLQIAETFFRDPL
                     GFPGVQMARVRRLQDRFDLRDLSLAARWREPLVEDADSAAQIAALRAGAPRFNVIVYY
                     VDTLRADVARDARVMPGLARFRESSLDFDHAYAVGSDTLRSLPALTGGNYDVLQTPPN
                     DLLRVAPRAGYETAIAIAKSAYEFLEKQRPEFHFATRFVVPDYPAELQVWGYGAQQPT
                     AAHLVDHAIEFLDRPRDKPFLLWLFNFDQHNWRQLEDQYVDEQARQFGVKEETGSAPL
                     RYRSVARSIDAQFERLLSALAVRKLTERTIVLFVSDHGEGLGREGFWAHSVFLWEPLV
                     RVPLVLRVPGIAGRRLSDRVSLVDVAPTLGRYLEPGLDGQGFHGQDLLGYLLPAPPRR
                     RHPLLMVSASKDMLVRIGMIDPYEPFKLVVSFESALPELYDLRAREPDDRDLAAQETR
                     RVRQGLNLLAHSPLFPRSAEDFDVRDTKEQKAQTR"
     gene            complement(22399..>22754)
                     /locus_tag="VFQ61_06120"
     CDS             complement(22399..>22754)
                     /locus_tag="VFQ61_06120"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9954057.1"
                     /translation="TLIELLNLGSAAYRRLVRRSALRRISRESLQRNAAVALGNSNDP
                     RAVAPLARALRSHPSALVRAHAAWALGQLRRWLDRDARDALKEASSHDDDPYTRESAQ
                     RARLGLVTESEPSAL"
BASE COUNT         4171 a         6980 c         7480 g         4123 t
ORIGIN      
        1 ctccgggggc ggtgccgctc ctcgttccac cactaccgtc tccgccgcgg ctgggttcac
       61 cctgcggatc tgtggtccgc cccgacgtac cgcctctatc tactccatga tcgatctgga
      121 gctcgtccac gtagactccg cctggggttt gttggcaagc gaccgctacg agcgcggcgc
      181 tgaacgtgag cagaaagctc ttcgcggcgc gccactcgag cgctcgcgaa cggctcggcg
      241 gtgcggagac ggtgcgaatt ccgttcagag tgcgcgtacc aagcgccgca cgagtggggt
      301 aacagtcagt cacatcagct ccatggagtc ggggttgcca ttcgacggca agcaaaaagg
      361 gccaccattg tcgcatcgca acgaggcgcg ggggtagtca tcgcattgcc cgcgtccgtt
      421 tcggcctcga agcgaggatg ctgtcgggag gccgcatgca attgcgagaa tggggtcacg
      481 aacgcgagaa ctactccgca gagttgcatg tgagaagatc ctcgaggaga gagagcggtt
      541 gcggggagga accgcacgag aaaagttacc catggcggcc gcgtcggtga ctcgcgacgc
      601 aatggcttca cgacaggggc tgcttggatg tgcaatgtgc tgcactttgc gaggacgtcg
      661 agttgcactt catggtacaa gcctggtggc ccagacggtg gccgaggttg attgaggcac
      721 tcactttcct gctgaattct cgccctggcg agtttcgaag cggtccatga ataggctggc
      781 aagcttattg caaaatgtag ccatgtggat ggggagtctc cgacccgagc agcccagatc
      841 cgatcagccc ggtccgaagt ttagtccgga aactcctgcc ggaagttcag tccggagttc
      901 aaggtgagga atcctttcgt aactcgcagt cgcgttttgc ggttggttta gttcactacg
      961 gctccggcgg ccctgcacga gcgaacacca cggaagcgga agggctccgt gtgtacgcag
     1021 ccaagcgaaa gacgcgaaac aagatcatgc gaaccgaacc tcagcaccga atgactcgca
     1081 ccatgatcga ccaaaaatct ttgattctgg ccggtcaaga cacttcacgc ctccaggcgg
     1141 ctccggctgc gggcgctgcc tccctggcgg tagccactct gatggccgcg tgcatcgtcg
     1201 gctgcggtcc ggcggagcca agcgccaatg acatcgagtc cttggaacag tcagccactt
     1261 tgagcgcagt cgccaaggtc gcggatacga cgcgagtcac gctcgcggcc gcggagctcg
     1321 ccagagtcgt gccgaccagt cccgccaagg attgttcgat gttcgacgcg tacacggcgg
     1381 cgctcgcgac gagcgtcatc gactgcctgg gcaccatcgg cccagacgta tttgcagtcg
     1441 acgagaaggg ctatctgaat cgacgtttcg acaagtgctt ggaaaactcc gaagagcagc
     1501 tggccaatat cgacggtctg ctctcgttgc aggttcgaga gcaccagcgc ccgaacaagt
     1561 ccggtattcg gctggatccg gtggcttgca tctccgggcg ctaccaacgc tgggctggct
     1621 cgttcaaagc agcggggatt cgcgagtgcc ctcgttgggt gaagaccggt gtgatcggcg
     1681 agactacgcc cgaggtggtg gccacgtaca ccaaactgtt gccccagatc agcaccaagg
     1741 gcacccgcgt ggagagagac ccgccggttg caaaacgcga ctacgcctac aaggtttatt
     1801 tcgcgagcgg gatccaggac aagggctgtc aggacccagc cagctgtgcg gcagcttgtg
     1861 tgagtggctt ccctggagcc ttggtcaagg catcgggaga agaggtcctg ctggacccga
     1921 cgtactggct cgatgcgacc aaaacgacgg tctacaccgc gggcggctat tatcacggta
     1981 tgtcctatta cggtggtccg cccggagacc tgttcggcca catcaatcgg cagaacgaaa
     2041 cctgcagcta ctacgatttc gacttcgaca tgcactattt gggcaagctg aagccggttc
     2101 agatcggcgg catgtttgcg agttggatga ccgagtgtat gccgtgagtt ttggatagac
     2161 gagttttgga tagagaggtt cgaaagctag cgagcctctc tttcggtctg atctgggggg
     2221 tttacggcgc ttgcgagttg agcgacaagc gggccgatgt ctcttttgat ctacggcgcc
     2281 aacggctaca cgggtgagct catcgcgcgg cgcgctcggg cgattggggt ggacccggag
     2341 cgggtaattt tggccggtcg caacgcgggt tcgatcgcag ccctgggaca gtcgctcgga
     2401 ttcgggacgc gagtattcgg gctggacgac ccggcgcggc tggatgccgt cctggacggc
     2461 gtgcaagttg tcttgaactg cgcggggcca ttcattcgaa cggcgttgcc actagtagaa
     2521 gcctgcttgc gccggcgcgc tcactacctc gatatttcag gtgagctggc cgtgctcgag
     2581 gggcttgccg ggcgagacca gcaggcgcgc gcggctggaa tcactctgtt gccggcagcc
     2641 ggcttcgacg tggttccttc cgactgtttg gctgcggaga ttcatgcgcg gctccctgcg
     2701 gcggtcgagc tgactctggc gttcgaggcg cccgagggaa tgtcgagagg cacggccatt
     2761 acgtcactcg agcagtctcc gcgcggcggt gtcgttcggc gcgacggcaa gttggtccgg
     2821 gtgcccaccg gccaccgcac gcggcagatc gattttggct cgggcgagcg aacggccatc
     2881 tgcattccgt ggggggacgt cgcgacggcg tactacacga cgggcatccc gaacatcgaa
     2941 gtctacgcgg cgttgccctg gccgatgcga gcggccatcc gagcctccaa ctggctccgg
     3001 ccggtgttgt cctcgaaggg cttccagcgt ctggtcgtgg accagctgat ggccggggcg
     3061 acgggtccga gcgacaccca acgagccagg ggtcaaagct tgctgtgggc ggaaggaaga
     3121 gacgacgagg gaagaagcgt gcagctccga ttacgtacgc cagagagcta cgagctgacg
     3181 agctggacgg ctctcgaact cgccgaacgg gctaggcacg gaaagctgcc cgtcggcttc
     3241 caaacgccag cgcgcgcctg cggaaaaggc ctgattcagc agttccccgg ggtgtcgctc
     3301 acggaagtgc ttgtataagt gacaccgatg cgaggcgtgg ctcggccggg caaccaggga
     3361 ttgcgagtga ggagtggggg aatgtggcag agcttgggtc ctgctttttg cgtgcacaaa
     3421 ctgcatctac gcagtgggcc tgcacagatg gactgcgata tgaacgtatc gagcccatgc
     3481 aggatcagct tgagtcgccg cttcagcgcg tcatcgccca catccgagat aagcgcttca
     3541 ccggattact tcgtgttcga gcccaagagg ccgaaggcga gctttggttc ttgtccggca
     3601 ttcgagagga cgtgaagttt ggcctgagta agggtgacga agcgctcgat cgcatgctgc
     3661 gcgcgacgca gccgagcttc gaagctttgc cgcgcttgcc cggtttgaac gccggtttca
     3721 agaaggcctt ggcgctgacc ggtacgttca ccgaagccgc gccggtagtg ctcatgcagc
     3781 actgcgagac gcaggcgttg acgtgcgtgc tcgagctcgt agctgcaaat cacgtcgtcc
     3841 gtgcgaagta tcaggtcggg gaattgttgt ccctggacgt ggactcagga ggtgacgccg
     3901 cggtcactga gacgttgaac gcggcagagg gcacgtaccg cttcacgttg ccggatttcg
     3961 agctgccgga tggggtggca ctcaccgcgc cgagcccggc ggagcgcccc gatatccggg
     4021 tgatcggcgt gccggctacg gacggcgagc aagcggcggc tcgcaaggcc gaggccgagg
     4081 cggaagctcg gagaagggcc gaagccgaag ccgaggcgcg ccgcaaggcg gaggcagagg
     4141 ccgaggcgcg tcgcaaagct gaagccgaag cccgtaggaa agccgaggca gaggccgagg
     4201 cgcgtcgcaa agccgaagcc gaagccgaag ctcgtcgcaa ggtcgaggcg gaggcacgtc
     4261 gcaaagccga agcagaggcc gaggcgcgtc gcaaagccga agccgaggca cgacgcaaag
     4321 ccgaagcaga agctgaagcg cgccgcaggg tagaggctga agcacgtcgc agggcggagg
     4381 ccgaggctga ggcgcgccgc aaagccgagg ccgaagctga agcccgcagg aaagccgagg
     4441 ccgaagccga agcccgtcgt aaggcggaag ccgaagaaga agcacgccgc aaagccgagg
     4501 ccgaagcgga ggaagcgcgt cgcaaggccg agctcgaagc ggaggaagcg cgtcgcaagg
     4561 cggaagctga agccgaggag gctcgcaaga aagccgaagc cgaggaagag gctcggaaga
     4621 aggctgaagc ggagcaggag cgtcgcgaag cggaggagcg acgccgagtc gaggagcgca
     4681 agcggcgtga ggccgaggag gccgaggaag aggcggcgct cgcggagcgc ccgagccgac
     4741 gcgtgttgag gacggggtca acgccgccag ttcccaagtc gggcccgggc ccgctcatga
     4801 ttttgctcct cctcgtgctt gcggccgcga tcgcctacgt ggtcgtgggc cttctccggc
     4861 gctgagccaa cccgcgcgag gcaggtgcaa cacagccctg ggttcggcga gcaacaggtg
     4921 aatcagtcgg ttgatacaac tcgacgccgc agcgcctgcc tcaacgaacc caagcacctg
     4981 caggaaatgg cctaatttag gcgaaagaac cggtttcaca cgtggcctcg gggcgggcct
     5041 cgtgcgctgc ccgggcccat ccgctccctg gcacgctgag tgcactacct ctcggcgtgc
     5101 aggcgacgaa gacagaacgg cagcagagga gcaggtctcg atgggtaacg agcctgctcg
     5161 gtgagcacgg gggcggggtg tttcggggta agtgccgccg ggccaacgtg gggtggctgc
     5221 gctccttcgt cgggtagtct ggcgtcccgg gggtcggctt cgcgggtgag gcaatccgcg
     5281 tgggcaagtc gcgggggcgg tggtggtgtg ggatctgagt tttgcgattc agaagccacc
     5341 ctcgggacgc cactcttcca taatctccca ttgaaaaaag tttcaggaaa caatttggag
     5401 cctgtgaaac gattccgaaa tattggatcc gtagggtgaa ctcgccctgg gcccggcatt
     5461 cggtccgtgg gccccgctcg tatccagctc gataatcggc tttctcggtt tttgattgtc
     5521 aacgcgcttc gctcgagaca aatcggcgcg ttgaaactca ggcgaaacaa caccgacgaa
     5581 atgcggaaag cgagatccgg ctacgcccag gcggccgcgc gacgcaacac tcgccgggcg
     5641 tttcaaaaaa ggaatcgaat caccatgacc ggtagctctg tacgcaagca agcgatcgcc
     5701 gagatcgcaa gtcagaagca ggtggcctcc gccccgtcga aagagtccgt agacgccctc
     5761 ttcgcctgca acgtgttcgg tctcgcggca atgaagaccc acttgccgaa agacgtcttc
     5821 cgttccttga agcgcaccat cgagaagggt gagcctctga acccggttca tgccgacgtg
     5881 gtcgcttccg ccatgaaaga ctgggcggtc gctcgtggtg ccacgcatta cgcgcatctc
     5941 ttctacccgc tgacgggtgc aatggccgag aagcacgacg cgttcttgtc gcccgatggt
     6001 gagggtggcg cgctcggcga gttctccgga aaagcactga tccagggcga acccgacgca
     6061 tcgagctttc cgaacggcgg catccgcgcg acggcagagg ctcgcggata cacggcatgg
     6121 gacgtgacca gctcggcctt cgtgatcgat tcgacgctct atatcccgac cgcgttcgtg
     6181 tcgtggacgg gtgaagctct cgataagaag acccccctcc tgcgctcgat gcaggcgttg
     6241 aatcgccagg ctacccgcat cctcaagctg ttcggccatg agaaccccgc tccggtggtg
     6301 gcctacgccg gccccgagca ggagtacttc ctggtggacc gccgcttctt cttggcccgc
     6361 ccggatttga tgaactcggg ccgtacgttg ctcggtgcca agccgccgcg cggccaggag
     6421 ttcgaggacc actacttcgg tgcgattccg gagcgcgtgt tggcgttcat ggtcgaggtc
     6481 gagcgcgagc tgttcaagct cggcatcccc gtcaagacgc gccacaacga ggtggctccc
     6541 gcgcagttcg agatcgctcc cgtgttcgag agcgcgaaca tcgcggccga tcaccagcag
     6601 atgacgatga tgacgctgcg ccgcatcgcg gtgcgccacg gcttcgagtg tctgctccac
     6661 gagaagccct tcgcgggcgt caacggctcg ggcaagcacg tcaatttctc cttcggcaac
     6721 tcgacgcaag gcaacctgct ggaccccggc gatacgccgc acgagaacgc gcagttcctg
     6781 gtgttctgcg ccggcgtgtt gcgcgccgtg catcgtcact ccggcttgct gcgcgcctct
     6841 gtggcgtccg ccagcaacga ccaccgcctg ggtgcgaacg aggctcctcc ggccatcatc
     6901 tccgtgttct tgggcgagca gctcgcggac gtgttcgagc agatcaaggc cggcggcgcc
     6961 aagtcctcgc gcagcaaagg caccctcgaa atcggcgtag atacgctgcc gaagctcccg
     7021 aaggacgccg gcgaccgtaa ccgcaccagc cccttcgcgt tcacgggcaa tcgcttcgag
     7081 ttccgcgcgg tcggtggaag ccagtccatc tcgggcccgc tggttgccgt aaacaccgcc
     7141 gtggccgagg ctctggatac ggtggcgagc aagctcgaag ccgcggtggc atcgggcaag
     7201 tcgctgaacg ccgccattca aggcgttctg accgagctcg cgaacgagca cgctgccatc
     7261 atcttcaacg gcaacggcta ctccgaggaa tggcacaagg aggccgagcg ccggggcctg
     7321 ccgaactacc gcaacacggt ggacgcgctg cccgtgctcg agcagcccga agtggtgcgc
     7381 atgttcgaca agtacgcggt gctcacgccc cgcgagctca agagccgcta cgagatctac
     7441 gtcgagcagt acatcaagac cgtgcaggtc gaggccaagc tggttgggcg catcgcgaag
     7501 acgcaggttc tcccggccgc tctccgctac caagcggagc tggcccaagg gctcgccgcg
     7561 gccaagagcg cgggtctgac gatcagctcc aagctggccg aggacgtgag ccgcaacatc
     7621 gcggagctcg agaaggggct ctcggctctg gatacggcga tgtcacatca cggtgcgagc
     7681 gacgcactgg cggaggctcg ccagttgtcc aaggacgtgc tttcggcgat gtcagccgtg
     7741 cgcacggctg cggacgctct cgaggtttcg gtggcggacg acctctggcc gctcccgacc
     7801 tatcaagaga tgctgttcat tcgctgatcg gaatttagcc accgcagctc cgtcaagggc
     7861 tcagcacccc tgacgtgagc tgctctggca agcccgggcg atgaaaatcc gggtgaacca
     7921 aggcgaaagg ccgactcgag tgattcgagc cggccttttt ctttcagcct cggattggct
     7981 tgtttcccga gtcaggcttg gggggaggtc ttctcgccga cttcacggcg gaagcggtcc
     8041 ggattcttga atagctcgtg aatccgcgcg atggacatct cgccgaggcc accgggctgc
     8101 ctccccaagc agcactgaat gaggatggct tcgcggagga tcggcagaaa atctgcgctg
     8161 cagcgggcgg cccacttgtc ggggggcatg tccgctcgac gctccgatgc ctcgaaatca
     8221 acgaccaaga tctcgagcgg cgccggacac accacgaagt tatgcagctc ggcgtccccg
     8281 tgggtgaggc ccttctcgtg caactgctgc aaggctcgaa aggcgccgtc gacgaagccc
     8341 caaaggcccg ccgccgtcgc gacggacagt ttgcgcgcga gcgggatatt gctctcttcc
     8401 ggctccaccc cgaacggcga gaggcgatgg tacggcgtac ccggcagaaa acagctgcga
     8461 aagacgtcgt tggagcaacc gatgggcggc ggcaaatcca agatggccga cgcttccgct
     8521 tgtcgatcga actcctggcg ggggtcacca tgcaaccgac ccgggaagta ggtcttccgg
     8581 aaatgcagcg ggtactgggc ggcgatgggg aactcggcgc gcgtgtcgct accggcgatc
     8641 acgatgtccg cggatacgag cggcagcccg ttggcttcgc gtcgcatgat cgttctcacc
     8701 acgaagtcgt ggcgcggggg gcgcttcaaa agaatcgcgt actcattggc gaggcgaacc
     8761 gagtgcctcg gagacaccgc gaccagctcg tcgtcgtcac tcacgtctgc ctcctataaa
     8821 gagcccccgc gtggtcgaaa gcatggctca cgcgctggat tttgcgttcg cgcgagcggc
     8881 gaaagtgagc cgctctgctt ccagcccaga ccgcaaaatt gcctgagatt ggccatgact
     8941 caccaaaagt cacggtggca gatcccctcc ggcgaaagca agcgattcca cggcccaagt
     9001 gaatctgtcc cacgcgactc gtgggccggc ctgtttggaa tctagtgcca atctttggag
     9061 ctgacgccgg tctcgaaccc gaaacgtcag gtcgtcgagc gcatcgaagt gtctgccttc
     9121 atgcgcaccg tctcggggcg cttcccgggg actcggagct gctgtttcgt cgcagtagct
     9181 cgcgtagctt gcgtcgggca cggaacaggc gcgtccccac cgttgccggc ggaaggccca
     9241 actgtgcggc cacgtgcgtg agcggcagct cacgggatag ctcgaaaact cgccgaaacg
     9301 gcgcggaaag ctgctgggtc gctctcgcta tgtcctccgc gctgaactcc acccaatctg
     9361 cctcgggtgt ggtctcggcg tccgggagtt gactgctctg gagctgcatc cactccagaa
     9421 cccgcaagct gaccagagct ctcgaccgcg ctcgctgctg ccggaaccga tccacggcca
     9481 agttccgcat cacggcgcac acccaacgca gcagatcttc ctcagacacc aacggagcgc
     9541 aacgaagggc gcgctccgcg gcatcttgca tcaggtccga agcttcggct cgatcctgag
     9601 tcagcttgaa tgcggccgtt tgaagactca gccggtggtc acgaaacaag ttcgctagaa
     9661 cactcagccg gaacgtgctc tgttgggaga ggcgctcggg ggaagcggcg aagcggtggg
     9721 agcgggctgc cttggacacg cgggttcccc cttttgaacc ggcggcgccc gggattcgat
     9781 ttcacgtcat ttcatgggcg agttattcgc gttaaccgtc gggaatattc gccaagcgat
     9841 tcgtataggg ggggtacggg tgcatgcgct gatgcgcgct gaatgggcgc aaacacccaa
     9901 tcgcccccga tccgttgcgc ccggcctcag atctcgccga cgcagccaag tagcgctcct
     9961 tagccgcggg ggactccagc tctcggggag tgacgaacgt cagcctacta ggcgctgact
    10021 acgtcaggtt actgagtggc ccggtacaca gctcgggccg accaaaggtg gtgacgggaa
    10081 cgcccatcgc gttcatgacg ctgaccaaca ggttggagtg cggggcgttc tgataggtga
    10141 gatagcgacc ggtccggaag tgccacccgc cacccaccat caagaacggc atgttttggt
    10201 gggaatgcgt atacccgagc gagacctcac tgccccaaag caccagcgta ttgtcgagca
    10261 cgctcgtgcc gtctggctcc tgataactcg cgagccgcgt cagaaggtag gccaagcgct
    10321 ccgcgtgcca ggtatgccgc cggacgagtt gctgtttggc agattcgtca ctcacccctg
    10381 cgtgtgagag ggtgtgaccc tcggacgtgc tgcccagcca tggatagccg atgcgattga
    10441 gcgagctcga gatcgcgaac gaggccacgc gagtcaggtc gcaggcgagg gcgatcgcca
    10501 gcaaatctag ctccaaatcc gcaatttgcg gcatgttctc gaccagcgtc ggatcgagag
    10561 acggcggagg agtgggcgcg ctgcaagcgc cgccacccag atcggtgagt cggcgctcga
    10621 cgtcacggac gagcgtcaag tgcaggtcca gcttctgctg gtcctctttc gaaacctgtt
    10681 gggagagggc gccaaactgc gcgcttacgg cgtcgagaac cgatcgtcgg ttagcgcgca
    10741 aagcgtccac cgcgtctcca ccaccaccga gcatcgagaa cagccgattg tatacgtcga
    10801 atgggttgtt gagcggtggc aggggttgac cggggccggc gtaggagatt cgcccttgca
    10861 cgtcgttgtc cgtcgcgtgc aatccgagct ccaagctcgt gagcagcgtg tctttgccga
    10921 tctgctgcgc gacggcctgg tccacgctca tgccatcggc ccagcccgac gtctgaccgc
    10981 agccgtctac gaaagcagtg ccggtttgca gctgctgccc cgtaaagagg ccgccgatac
    11041 cgcgttgatg cagtccaccg gggccttcgg cggccacccg caaatccacg ccgctgaaca
    11101 gcgttagtcg gttcttgaag ggggacaagg gttggtggca agccgccaga tcgaagtctg
    11161 tctccgaacg ggcattttgc ggccaccacg cgtccgggat cacgccgttc ggcgcgaaca
    11221 tcaggaccaa gcgcttaggc ggggagggca cctccgcctt cgcctgcacg gaccgcaaca
    11281 agggcaaccc cagagtcaat ccaccgagcc cacgcagaag ttggcggcgg ttcaagcgct
    11341 cttggatacg ccgcgacttc atgggttgcc tccgatggct gggcggtagc gaaaggcgat
    11401 ggtcttcggg atcgaaacca acaactgttt caagtcacct cccgatgcgc tgaactgagc
    11461 cttgatgtcg tccaagctgc acttgtccgc ttcggtctcc atgcgcccca tcgcgaatcg
    11521 gtaccagttg gtggcgacac agtcgcggac gcggtcgctg gtttccaagc gatcggcgag
    11581 ctcgttcacg ccgtcgaaat cgccgtccag cacggagtcg ccgatcacga ccgtgcccga
    11641 ggcgtcgatc gggattccgt tctcttccgt gcgatagcgt ccgagctggt cgtaggcttc
    11701 gaagccgaaa cccacgccgt cgatcatccg gtgacagccg ctgcaggccg tgcttgccgt
    11761 gtgcacgcgg aagcgctcgc gagtcgtcga gttcggatcc gggtcgggtg gggtattgtt
    11821 gacggaggga ggagggttcg gaaccgccac gcacatcagg cgtttgagcg taaaaacgcc
    11881 gcgcagcacc ggcgcggtct gatcactgtg cgacaacagt ccgagcagcg ccggctgcgt
    11941 cagcagaccg cggcgatcgg acagcgtaac cgcttggaaa tcggagtcag acggctgcgc
    12001 acctgaaagc ggagcgccgt acagcttcgc gagcctgggc gtcacgaaga cctgcttcga
    12061 gttgaacagg gtctggaggt ttccgctatc ccagtacacc gtgtcaatga agcgatcgag
    12121 tgaaccgagc cagtccttgg tcagcaaggc ggcgtcttcg ccgagttcgg gcgccatcgg
    12181 gctgagcgcg gcgatggcgt ctaggccgag ccactggtga tgaaactccc gcaccacctc
    12241 tttcgctcgc gatgtctcga gtaaccttcg ggcttgagcc tcgatctctt cgtccgcgct
    12301 gaggcgcccg gcgcgtgcgg cctccagcag ctgtgaatcg ggcgtggacg cagtcagaaa
    12361 gtaggacagc ctggccgcga gctcgtagga gctgagcgcc acggccccgc tcgcctccga
    12421 cggcgcaccg cgcgaatcga cgcgatacag aaattggggc gattgcagca tggcctgcag
    12481 cgtgagctcc actgcgctcg tgtatccgag ctgggcttgc actcggccaa acagtgcgtc
    12541 gaagatgctg gtctccgagg caagcagagg ccttcggaac gctcgcaagc cgaagttgcg
    12601 cacgaagtcg cgcccgcact ccacctgctc gcggccagcc tcacaaggag ccaatgcgct
    12661 ggccatttgc gccgcggagc ccgctagccg ttccgctgct tccaggtatt tctccaccaa
    12721 gaccgggctc gcttgattgg cctgggtgtt gttcttgaag ccctccacct ggttctcggg
    12781 agggaagtcg gcggccgcgg acggcccatc accgagcaga tcccgtacgg tgttttcgta
    12841 ctgcatgcgg gtgagcagcg acaagggcgc ggtcggtggg ttcaagcagg cgttcacgcc
    12901 gcccgcaccg gccgccattc ccccattgcc tgcgccattc gatgatccgc cgaacgccgc
    12961 tccactcgct ccgcccggtg agccgttgtt ggccgaggat tccgcgcaac tcgagctcaa
    13021 tagcccggcg cacacaccaa tcaggacaag cgtcgagcga ttcatggggc ggcttcagtc
    13081 aagcgtgccg cgggcatcgt acaccgtcaa ccgcgagcac gctcgccact gatgatcccc
    13141 tgtggagtga tctccccaga gctatggtcc gccctattcg ccaagtggtg tcccccaagc
    13201 cctgtggggt gagcctcgcc agggctcggg ggcaagcggg tactctgaac cgcaagggtg
    13261 ctgggcgctc aggcctcgtg caccgtcacc ttcaacattt tcacaggagt ggtcggacga
    13321 tcgcgtccgt ccgtcggggt acgttcgatc ttctgcacca cgtccgtgcc tgcagttacg
    13381 cgaccaaata ccgggtgttt cgagggaccc ggtgaaaacc agtccaaata ggcgttgtgg
    13441 acggtgttga tgaagaactg gcagctgccg ctattcgggc ggcccgtgtt cgccatcgaa
    13501 agcgtaaacg gttcgttcga gatcttggcg ttttccggat gctcgtcagg aatggtcccg
    13561 tccgggccgt tgcccgtgcc cgcgcgaggg ctcgcgggat ctcggctgaa ggggcaaccg
    13621 aattggatca tgaaaccgtt gataacccgg tggaaatgta agccgtcgta aaagccgttc
    13681 tttgccagct tgatgaaatt ctgcgtggtc gcgggcatct tgtccgcgag gagctccacc
    13741 gtgaaatcgc cgagtgaggt actgaatgtg gcagtcggat tcgccatagt gccgacgagc
    13801 ctaattccgc cctggaatcg aggcaattcc cgtctgtctc ggagtgacgc gcggctcgac
    13861 cacgcggtgt ggccgcccgt tcggtaaccc cggtacgggc gttttcgctc gcccgtatcc
    13921 gtcggcccgt gcgccgagac ttgccgcggt cgaccaaact tgtttacggt gccgcgctcg
    13981 gagttcggga aatgaaggat ctattgcgaa cgctcgacct cagccggcag gatttcaagt
    14041 acctgctgag ccgagctagt aaattcaagg ctcatcccca ttcgcgtcac aagttgctgt
    14101 ttggcgacag catttgcatg tacttcgcga agccttcgac tcgaacgcgt atctctttcg
    14161 aggcggcgat cgctcggctg ggcggtaatc cgttggcgct caacccagct gatttgcagc
    14221 tggggcgtgg ggaaacgttg gaggacaccg ctcgcatcat cagccgttac gctcgcgcgt
    14281 tggtgattcg caccttcaaa gacgaagacg tgcatcgcct ggcgaaagcc gcgaccattc
    14341 caatcgtgaa tgcgctgacg gatgggcatc atccctgcca gagcgtcgcg gacatgctca
    14401 ccatccaaga gcaccgcggt tcattgaaga actgcaaaat cgcttacttc ggggacggca
    14461 acaacgtcgc catcagcttg atggaagcct cggcgttggt gggggcggac ttcgtcatcg
    14521 cctgtcccga gggctactcc atccccgaac cgttgatcga gcaagcccgt gcggtggcag
    14581 acgagacagg tgggacgatt cgtgtgactc gtgatcccga ggaggccgcc cgcggcgcag
    14641 acgccttgta cacggacgtg tggctctcga tgggcgacgc ggattcggaa cgcgaggccc
    14701 gcttcaacgc actctcgcct taccaggtga acgagcgcct ctcgaagctc gcggcacccg
    14761 gagcgatttt cctgcactgc ctgccggccc atcgcggcga ggaggtcacg gccgacgtga
    14821 tcgatggtcc gagctcgatg gtgtgggacc aggcggagaa ccgcatgcat acctcgatgg
    14881 caatcttgta cggattgctc gaaggtaaga tcgaaggtcg tcagtccgag cggcgttgac
    14941 gcacaccgct cgacgcttgc acccaggggc agcgtcaccc gaggcgcgat ggctcgtgtg
    15001 cggcgcgcta cttccaggtg cgcggggcac gtgtgcgttc tctcacgtgc ggcgacgtac
    15061 ttgcctggcg cggcgcccaa ctcggcagag cgccccgtga ttggggtagc ggcctcgctt
    15121 atcccagagt tgggcgcctt gtcagagcag cggagcgcac ccttcagcgc gggatgtgcc
    15181 ggccgttgag ctgtgagtga gccaggatct gttgtccggc accactcctt ctcggctggt
    15241 gtgcagccgc ggcaggtggt ctctggaaat gacgcgacta gtcgcggtgg acagccatgt
    15301 cttccagccg cgcaagcagc gtctagaggg cagcgaagcg atcctttcgc acctcaagcg
    15361 ccacgccgat tctgggaacc tggtgcaggg tgctgcccac ctgatgcggg ccgcccgaag
    15421 tgacgaggag cgatccttcg aggccgtgga cggggaatgg gtgcccgtgc aggctgcccc
    15481 ggcagatacc cactccgagc ccaatgccaa gcagcgcgca gcgctcgaga acgtgatcgc
    15541 ggagctacgc gccgaactct tgatgctccg cgcatcacac caacgcctgc gggagcgggt
    15601 cgcaactctg gaagcgcgca tcgaaaacgc cccacttccc gagaaaaagt cagctcgggc
    15661 cgagttggcc acgcccgcgc gtctgtcgga ggctcgctcc gcccggaccg aggtgaagtc
    15721 cgccaccgcg gcgcacaagc cggcagagcc tctgagtgag ctgggcctcg cgccacaatc
    15781 cgaccgagac ttgctgggag tgctggccga cttgttggga gaagactcgg gctacgctca
    15841 gatacaacgc gccatcccgg aatcgtattt gcaacggctt ggcatgtacg tatcgctgct
    15901 ggtcgacgaa agcggccgag aggttggcgc gatcgtgtcg catgtggcct gcaccgccga
    15961 gctcggggga cgccttgctg ggctcacgcc cgaggaacgt caccagcaag cgcgcagcgg
    16021 tgaactgagt gaaaccgtga ctcaggccat gagtgaggtg tgcaacgcgc tcgcgggggt
    16081 tctggctcgg tcggcgaagt ccccgggcgt acgttgcaca ccgctcgagg ccttcgatcc
    16141 agatagactg ggctggctcg gcgaagcgcg gcagcggctc tgtttgagca agccagacgg
    16201 cggcgcgttc tgggtgctgg gccgctgacc ctttgagttc gtcgcggaat tcgcgagcgc
    16261 tggagttgcc gcggcccacc agtacctagc gtgagcgcgg ctcaacgttg cgacaaggcc
    16321 gtgctcggcg gcaggcgtcg cgctgggact cttctcgcgc tcgcgggcgc gagcggcgta
    16381 cgaccgcgga gcgggatgcg gtactcgcgg ttctcgcgga tgcggctatt tttcaggcca
    16441 ttcgcgcgct gaatgctttc gatgctgacc gagaaacgct tcgaaatctt cttcagcgtg
    16501 tccccgtgct tggcgcgata cttggcgtag atcgtaagct ccgggattac cccggcagcc
    16561 cgcaacgccg cgtgagcacg acgacccgtt tcttctgcaa ttgggttgta aaaacggatg
    16621 tggatgtggg tggcgtgacc gggggcatgg cgtaccagcg gcggcgtgga cggcgcgctg
    16681 cctcgaaaga cactctcgac ccaggccgca tcttcgccct gacccaacgc atactccttg
    16741 agccatgctt gaatcgagtg atcgatcagg atcatctcga cgtccgtctc cgttacgagc
    16801 gcgcgcacga aggcccatgt gcgggcaacg tccaggtttt ggagcgtggc tcgcgtgtac
    16861 cagctcttgg gttcgcgata aaaaaacgaa atatcgacgt cgcgtcccgc ctgatggctg
    16921 acgtgtggtg agagggggcc accgcgttcg gcgctgatgt gcccgatcac gagcggtgca
    16981 ctatcgggat aggcttggtg cactttgcgc agcgcgcgct ccaagtaaac gagggtttcg
    17041 cgcgtgccaa acgcatgccg cggatcgacc agcgcgtaca gttcggatgc gtgcgcctgc
    17101 tcgccattga acagcatgcc cgcattgggg cgcccgatcg acattggccc cagcgacttg
    17161 ggatcttggc gtaccgcgct cgtaattcgc tccgccgacc agccgtcgag agggtgaggt
    17221 acgggagccg cgctgtctgc gctctcgctc acgacgtctt ccacggactc atcctcgacg
    17281 agctcgctgt ccgaatcggt gctctcgtcg tctgcgggag ctcgcgacgc tgtctctgca
    17341 tcctcctcgc tgtccgctgg ttccgagagg ccactttctt cggaactcgt ggcgaggctc
    17401 cgagccgagc cccgggactg cggagcgcga gcggtggggc tcgggaggat gctagcgcat
    17461 ccccctagca agcctaacca cacgagcagg aatgccggtc taacgcgagc tcgcatagaa
    17521 tgcgtgggtt tggcgtagcc cctcctcgag cggagtcagc cgttcgatgt agcgcagtgc
    17581 gagggttggc tggatcacag agcgttcgag gtcccccggg cgggagggag ctggctctag
    17641 aggtacgctg cgccccgtca ccttcaggat ggtctcggca agctctcggg tagtgattgg
    17701 aacaccggtc gctagattca cgacgggctc cgtgacttga ccgtaaagcg cgagtcgatt
    17761 gacctgtgcg acgtcggaca cgaagacgta gtcgcgcacg catcccgcgt ctcccacgga
    17821 ccgccgcgcg ttgatgcgaa tgcctttgcc gctcaaggct gcgtcgaaga agatcgcgat
    17881 cacgccggct tcgccgtggg gatcctggcg tgggccgtag acgttggcgt aacgcagcac
    17941 gcgtgtctcg agaccacgat gttttgcgta cacacccatc agttgctcga acgcgagctt
    18001 gtggatggcg taggggctct gcggattgga tccggtggct tccgtagcct gcgtgccttc
    18061 cggaacttcc ccgtagatgg ccccacccgt gctcgcgaac acgaagcgct tcacgttggc
    18121 tccaggcgcc gtgcaggcat ccagcaaatt gagcccgcca aggatgttca cctcggcgtc
    18181 tagatgaggc tcacgcacgc tgacggccac gctggcttgt gcagcttgat ggctgaccgc
    18241 ctcaggacgg aattccgcta ccaactgccg caccgcttcg cgatcgcgaa ggtccagttg
    18301 tcggaacgcg gcgcccgcgg gcacgttttc gcgccgcccg ctgctcaggt tatccaagac
    18361 catgacctcg tgaccctccg ccacgaggcc gtgaacgata tggcttccaa tgaagcccgc
    18421 accaccggta atgaggacgc gcacgacgcg ggttatagcg caagctggcg gaagtcagtg
    18481 gctcgattgc acttttcgaa acgcgggagc aaccttgcgc cgtggaatta gaagctttgt
    18541 cggatcctct ggcggcgctg ctggcgtggc accgcgaagc gctcgaaaaa aatctaccgg
    18601 aacccgatgc gtgcacgttg gccaccgcca cgctgagcgg caagccctcc gctcgcatcg
    18661 tattgttcaa gggaatcgtg aacggggacg tgcagttcgt cacgaattac gccagccgca
    18721 aaggccgcga aattgccgaa aacccggccg tggcgttggt gttctattgg cacgcgctgc
    18781 tgcgtcaggt gcgggtggag ggcgaggcat ccttcgcaac agccgaggaa tccgacgctt
    18841 actttcgaac ccgcccgcgc gagagtcagc tcggggcatg ggcctcactg cagagccagc
    18901 cggtcgcatc gcgcgcggag ctcgagcgcc gcttgcgaga ggtcgagcaa cgcttcgaag
    18961 gcaaagaagt cgagcggccc gagcattggg gaatctatcg gatccgcgtc gagacggtcg
    19021 agctctgggt cgcaggcgcg catcgactac acgaccgctg cctgttcacc cggaccgcgg
    19081 agggttggcg gccggctcgg ctttttcctt gaggttctca gcgactcgtt tcgccaatga
    19141 cgctctggga cggggcgagc ggtcgagcaa ccacgctcac aaggcgcgtt tggggcgcag
    19201 cgagtgttgg acggcgctcc aatgcacgga gggcgttcgg caaaacgctg ccatatgacc
    19261 gggcgcctct gagcgcgttc ccgtctcaca cccttccaga gcgttgcgcg ccaagaattc
    19321 gtccaagtgt ttcttgaaat gcgcggcggt caggggcgcg gccgggccga agaagtccca
    19381 caggaacgtg cgctgatcga cgagagtctc agtcaccatg tcactccgaa gtttacggaa
    19441 agaacatcag cgccacgaat aggatggccc agacgatcga caggaaatgc cagtactgaa
    19501 cgcaaagcga aatgccctcg tgatgtgagc tcgaatactc cgcttggcgt gcgcgcttcc
    19561 acacccagcc gagcggaatc aagccgccaa tcacgtgagc cgcgtgaaga cccgtgagta
    19621 gataaaacgt aaaggtgtac agcgtgtgca cgccgagcaa gcgctcggtc cgaatcaaag
    19681 agtaccagtt ctctccttgc accagcacga acaccacgcc gagcacgaag ccgagcgcga
    19741 ggcggctccg gagggagccc aagcggttcg cgcgcaccgc cgaaagtgca gcctgcagag
    19801 tgaagctctg ggccacgagc aacgcgctgc tgagccacaa gccccgtggt gctcccgtca
    19861 tctctggcac tcgaaagacc gccgcttgcg cgcgggtgat gaagtagccc acgagcgagg
    19921 ccgcgaagaa catgctcagg gacaaaagaa gcacgtacag acccatgcgc cgaggcgaga
    19981 gccgaccgat gaaggagcgg cgttgggaca cctcgattgg ggagggtagg gccatgggca
    20041 tggtgagctc gacggagaat tagattctta gcattgggcc gcgtctcggg gtgagcggag
    20101 agtgtgcttc ttcgagacgg cttgcggcag cgagaccgat gggaagctcg atcgggacgc
    20161 gcgcgctcac gtgggtcagc gaaaaatcgc ggtcggtagg gggataagac gcgatgggcc
    20221 ccgtgacaag ctggatcgtg ccgcctatgc tccgcgcccg tcgagctagt gaaatgctcc
    20281 tgaagccgcg ccaaacgaac cagctctcga aaccccgaac cgcattacca ctcgcgctcg
    20341 tgagcggcgt tttctcggcc ttttgtttgg tgcttgccga tctggtgcat cgcgacaact
    20401 tcgattacgt gccgctcggg cgtcatctat tcggggccct cgggatgtat accggggtcg
    20461 gggtgctgtt tggggtgcta ctccttgggc tcgtcagctt ggagtcccgc ttgatcgcgc
    20521 gacgcgccac gccgcgcgcg cgctgggctc ggccgttctt ttatggaatc gtcggcgcgc
    20581 tcgccagcct cagcaccgca atttcggtgt tttcagggga acgggtccag aagacattgc
    20641 tcgtttacgt cgggcccata gtcttctccg gtgcgctcgg cttggcggct ctcatcggtt
    20701 cggcattcgt tctgcggatt ttagccgctc cgccgcgcgc ggccgtgtcg ggcgctctgg
    20761 gctttctcgc gctcggggcg ctcatcgcgc gtgtcgacct gactcagtat gtggccctct
    20821 actcgcgcat tcatacgctc ttggaggtga ttgcagcctt gtgtttcggg gccgccatgg
    20881 tgatcgcggc gcggctcgcg cgccagagcc gtccgagcct cgacgtcaca ctggccgcgc
    20941 tatccttgtt gttcggagca tggttcgtcg caaccctgtg cgtgccggcg ctgcgccgga
    21001 gcttcgacga agccttgcgg catgtctggc tagacgaggt gtacgccggg agaatgctgc
    21061 gacgactcca aattgccgaa acgttttttc gcgatccgct gggtttcccg ggggtgcaga
    21121 tggctcgcgt tcggcggctc caagatcgtt tcgatctgcg cgacctgtct ttggcggcac
    21181 gttggcgtga accactggtc gaagacgccg acagcgcggc gcaaatcgcg gcgttgcggg
    21241 cgggcgcgcc gcgcttcaac gtcatcgtct actacgtaga tacgctgcgt gcagacgtcg
    21301 cgcgggatgc gcgggtgatg cccgggctgg ctcgttttcg agagagttca ctcgacttcg
    21361 accacgccta cgccgtgggg tcggacacgc tgcgctccct accggcgctc accggcggaa
    21421 actatgacgt gctgcaaact ccgcccaacg atctgctacg ggtagcgccc cgtgcgggtt
    21481 acgagaccgc gatcgcgatc gcgaagagcg cgtacgagtt cctcgagaaa caacggcctg
    21541 agtttcactt cgcgacccgc ttcgtcgttc ccgactatcc cgccgagttg caggtttggg
    21601 gctacggcgc gcagcagccc accgctgctc acctggtgga tcacgcgatc gagtttctgg
    21661 atcgcccgcg tgacaagcct ttcttactct ggctattcaa cttcgatcag cacaactggc
    21721 gccagctcga ggaccagtat gtcgacgagc aagcgcgtca gtttggggtg aaagaagaga
    21781 cgggctccgc tccattacgc tatcgatcgg tcgcgcgttc gatcgacgct cagttcgagc
    21841 ggttgctgtc ggcgctcgcg gtacgcaagc tcacggaacg cacgatcgtg ctgttcgtct
    21901 cggaccacgg cgaaggcttg gggcgtgagg ggttttgggc gcattcggtg ttcttgtggg
    21961 agccgctcgt gcgcgtcccg ttggtgctgc gggtacccgg catcgcgggc cgtcgcctct
    22021 cggatcgcgt ctcactcgtc gacgtcgcgc ctacgctcgg gcgctaccta gagcccggtt
    22081 tggatggcca gggatttcat ggtcaggacc tgctcgggta tctcttaccg gctccgccgc
    22141 gacgccgaca tccgctgctg atggtgtcgg cctcgaagga tatgttggtg cggatcggaa
    22201 tgatcgatcc ttacgagcct ttcaagctgg tggtgtcgtt cgagtcagcc ctgcccgagc
    22261 tctacgactt acgcgcgcgg gagcctgacg atcgagatct cgcggcccag gaaacgcggc
    22321 gcgtgcgtca gggcctgaac ttgctcgcgc attcaccgct gttcccgcgc agtgcggagg
    22381 acttcgatgt tcgggacact aaagagcaga aggctcagac tcggtgacga gcccgaggcg
    22441 ggcgcgctgt gcgctctcgc gcgtgtaggg atcgtcgtca tggctgctcg cctctttcaa
    22501 cgcgtcacgt gcgtcgcggt ctagccaccg gcggagttgc ccgagggccc acgccgcgtg
    22561 ggcccggacc aatgcgcttg gatgacttcg aagcgcgcgg gcgagaggcg cgaccgctcg
    22621 cggatcgtta ctgttgccga gagcgaccgc cgcgttgcgc tgcaatgact cgcggctgat
    22681 ccgacgcaag gcgctgcgcc ggaccaaccg tcggtacgcc gcagacccga ggttcagcag
    22741 ctcgatcaag gtcg
//
LOCUS       DASTKU010000170         6621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_478808, whole genome shotgun sequence.
ACCESSION   DASTKU010000170 DASTKU010000000
VERSION     DASTKU010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478808"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            574..804
                     /locus_tag="VFQ61_06125"
     CDS             574..804
                     /locus_tag="VFQ61_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET9954058.1"
                     /translation="MIVSLIDIYSLVLFAAVVLSWVQLPADNPLVRFVRALTEPLLAP
                     IRKLLPNVGGFDLSPMIVFGILRLLRRLVTGL"
     gene            complement(835..2886)
                     /locus_tag="VFQ61_06130"
     CDS             complement(835..2886)
                     /locus_tag="VFQ61_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9954059.1"
                     /translation="MTCHTFSMLRRLEVEGLALLSDVQLVLHPGLNVITGETGAGKSL
                     LMGALACLMGARAGTEKIRGDGGAARVEAEFELSEPLPRELHEMVGAGLRFSVRRTLR
                     RTGRGECSVAGRPVSVQQMGQLVRQLIAFNGQNAAYEELSSAGFIDVLDGLASRERGS
                     GRAEAGAREQEKATPREASPEPAIAESIENMKIGSAQPVPQNKKAKRSSQSSVVRGRK
                     VPVRKAERFRKSQLATPTPDSQVPGAAVSSGAALCGSAARALEELEGVAMDGRDMGSI
                     LAEYRDIYLSLRQVRSEAQRLFIEREQATVRKPLLTDWLASFESLEPRAGEVAKLEQQ
                     IGLARGARHFLELARRVQDTLSEREGSVEEELRTLVHAVSRGNDVQPFAAVAEALREA
                     LVVLNRAGDECRRITERLELDPAELESMENRLHELIRLSRRIGVAPSELAETRRALQE
                     ELELAEQLESAYAAASVEVERLTRKAVERAELLHESRSRWAAELERRITQELRALCIE
                     GARIEIRLTRRERSDLDGRGFTSAELLFSANPGEPLARLDRVASGGERSRVLLALRSA
                     GVLGAGSTMVFDEIDAGVGGAAAAAVGERLRHIATARQVLCVTHQASVAAQAQAHFHV
                     AKHVKEGRTETLVARLGEPERVAEIARMLAGDQRPAAARELASALLRSAGARVAAVRA
                     A"
     gene            4080..>6621
                     /locus_tag="VFQ61_06135"
     CDS             4080..>6621
                     /locus_tag="VFQ61_06135"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HET9954060.1"
                     /translation="MASLGALLERPKGGPLDPEIIAAPGQGMAVWLSMQLSKQLGVWA
                     APIRFPRELVDHLMLRVLGEDARELSAVSRDAMGFAIQAELPSLLNEPGFDGLRRYLE
                     QDEYGVRRAELAMRLAATFDQYLTYRSDWVRGFEEGALLDLPAGNQFQTLLFRRVVGR
                     LRRKHLAQLEPRLLDVLSGATAPASLPARISIFGISTLPPLFVRILAQVARHIEVHFF
                     LFSPGAHAWSPEAGTRSRHPLLERLGAVGADFDGVLSACASDADLTAIRHEHFVIPEP
                     NQLLTALQADLAGDPRPAAPELRLEACTATSSIAVHSCHGPMREVEVLHDQLLSLLTR
                     ARDPVRPEEVVVMVSDLDTYAPLIDAVFAREPGHPEFIPYVVSDTLLSLDASTLRAFD
                     RMLGMVRGRVTASEVLDILTVPAVLARFDLDLLEADRLRDWVCESGVRWGMDAEHRER
                     MGVPASDNNTFRLGMQRLMLGYALPTSGEALFEGVLPYEQIEGQDLVLLGKFSSFRRR
                     LFHWLRELERPRAMAAWVETLTQLLGDLFSESVESAARLSRIRKGLADLAAASLTARF
                     GDALEIEVVHKLLGQKLKAIAPEHAYLSEGVTFCAMLPMRNVPFRVVCALGMNDNTFP
                     RTPPISEFDLMQSNYQPLRPGDRSLREDDRYLFLEALCQARERVLLFYTGRSVRDNKP
                     RPPSRCVSELLESIERRLGVPARDVVVEHRLQGFSRAYFDARDSRLFSYSKSYLNAAR
                     SMARAQSSLDPFVGKPLEAELPDALRLEDLVRFFRSPPAYYLNRRLGIYLDEEDIDVS
                     DREPLELSALDNWRLGSSLIEHLLKGRSLQEAADLLQASLELPHGSWGT"
BASE COUNT         1134 a         2192 c         1973 g         1322 t
ORIGIN      
        1 gttcggttac tacaagcgtc tacccgtcaa cggatgttga gcgcggcatc ccgctcccta
       61 gtactccgct cgccacgctt ttgcccttcc ggctctccga cgcaaacatc cccaaaccga
      121 attggcgttt gcgccgcgaa tgattgagcc gttatgtgtc cccgaaacca cataccgatt
      181 cacagctcgg aacagatctg caatcggcga gaacacgagg caaaagcgcg aaaccgcgct
      241 gcactaacgc tcagctgacg gtgctggcct gggccgttgg ttgaaccacg gacacgactg
      301 cttcgatccg agaaggcacg cgaagcgctg agccagggac gcggctctgg gtccgttgct
      361 ctggtgcggt ggtctccgga gacgggccca gctcggttac caggctcggt taccaggctc
      421 ggttaccagg gctcggcgac caaagctgcc ggactcagaa gccccgcgac tgattcgcgc
      481 ttgaaacgcg gcctagcgat caccgggaca atgccaggcg agtcgctaaa tcgggagccc
      541 aaggcgcccc tcggcggtag tgtgagagcc ccgatgatcg tcagcctgat cgacatttac
      601 tccctggtgt tgtttgccgc cgtcgtgctt tcgtgggttc agctcccggc cgacaatcct
      661 ctcgtacgat tcgtccgcgc attgacagag ccgctgcttg ctcccatccg aaagctgctc
      721 cccaacgtgg gcggattcga cctatctccg atgattgtct tcgggattct gcggctcttg
      781 cgcaggctag tcaccggcct ctgactcgca ggagcagcca gacatccgcg gccactaagc
      841 cgcacgaaca gccgcaacgc gggcgccggc ggaccgcagc agagcacttg ccagctctcg
      901 tgccgcggcc ggccgctgat caccggccaa cattcgagca atttccgcaa cgcgctctgg
      961 ctcccccaaa cgtgctacga gcgtctccgt tcgcccttct ttgacgtgct tcgcgacgtg
     1021 gaaatgagcc tgagcttgcg ccgcgacgct tgcctgatgc gtcacgcaca gcacctgccg
     1081 cgcagtagcg atgtgacgca atcgctcgcc gaccgcggcc gccgccgccc cgccgacgcc
     1141 cgcatcgatc tcgtcgaaca ccatcgtgct ccccgcgccc agcacccccg cactgcgaag
     1201 cgccaacaac acgcgcgaac gctcgccacc tgaagcgacc cgatccaaac gcgccagcgg
     1261 ctccccggga ttcgcagaaa acaacagctc cgcgctcgta aaaccgcgcc cgtccaggtc
     1321 cgaccgctct cggcgcgtca accgaatctc gatccgggcg ccttcgatgc acagcgcgcg
     1381 gagctcttgg gtaatccggc gttcgagttc ggccgcccag cgcgaacgcg attcatgcag
     1441 aagctccgcg cgctccaccg ctttccgcgt caatctctcg acctcgacgc tagccgccgc
     1501 gtaagcgctc tccagctgct cggcgagttc gagttcctcc tgcagcgcgc ggcgggtttc
     1561 ggccagctca ctgggcgcca ccccgatgcg acgcgacaac cggatcagct catgcaaccg
     1621 gttttccatg ctctcgagct ccgcggggtc gagctcgagc cgctccgtaa tgcgtcgaca
     1681 ctcatccccg gctcgattca acacgacgag tgcctcgcgc agggcttctg cgacggcagc
     1741 aaacggttga acgtcattgc cgcgcgagac cgcgtgaacc agagttcgga gctcctcttc
     1801 gacactccct tcgcgttcac tgagcgtgtc ctgaacgcgc ctagcgagct ccaaaaagtg
     1861 ccgggcaccg cgcgcgagcc cgatctgctg ttccaacttc gctacttcac ccgctcgtgg
     1921 ctccagtgac tcgaagctcg ccaaccagtc ggtcaataga ggtttgcgaa cggtagcctg
     1981 ctcgcgctcg atgaacagac gctgagcctc tgaccgaacc tgtcgcaaag agaggtatat
     2041 gtcgcggtat tcggcgagaa ttgagcccat atcacgcccg tccattgcga cgccttccag
     2101 ctcttcgagg gcgcgcgcgg cgcttccgca tagagcagct ccgctagaga cagcagcgcc
     2161 cggcacctgg ctatccggag ttggggtcgc gagctgactc ttgcgaaaac gctcggcctt
     2221 cctgactggc accttgcgac cgcgcactac gctcgattga gacgagcgct tcgccttctt
     2281 gttctgtgga actggctgcg cggagccgat tttcatgttc tcgatcgatt ccgcgatcgc
     2341 tggctctgga ctcgcttcgc gcggcgtagc tttctcctgt tcacgcgcac cggcctccgc
     2401 tcggcccgat cccctttccc gcgaggccag gccatccagc acatcgatga atcccgcgct
     2461 cgacagctct tcgtaagccg cgttttgccc attgaaggca atgagctggc gaaccagctg
     2521 acccatttgc tgaacggata ccgggcgacc agcgaccgaa cactcacccc gcccggtacg
     2581 acgcagagta cgtctcacgc tgaatctaag ccccgcgcca accatctcat gcagttcccg
     2641 cggcaatggt tcagaaagct cgaactcggc ctcgacccgc gccgcaccgc cgtcgccccg
     2701 gatcttctcg gtcccagctc gtgcgcccat caggcaagcc aaagccccca tcaacagcga
     2761 cttccccgct ccagtttcgc ccgtgatgac gttcagcccg ggatggagca cgagctgaac
     2821 gtccgagagt aaggccagtc cttcgacttc caagcgccgc agcatactga acgtatgaca
     2881 cgtcaactga ttctatgtcc agtattttac tgaacgctcc taggcgtgtc agaccgggcg
     2941 ccttggtgga tgccgggcac ggcgagccca cgcggtagcc gcacgccttg cccgcgttcg
     3001 gcatgcggcc gagagagggc tgactcgagc gtccgctgac gatgtcggat cgtcgttagg
     3061 cgctcatccc ttagatggtt tggctgtctt ggctagtttc gccggacgtg ccttcgcgac
     3121 gcgagtcggt ttggcatgat ggccccccgc tgctttggca accttcagct ggggctttct
     3181 cggtccagcc gcgcccgcgg actttttctg tccgagcttc gaccccagcc cagtttctcg
     3241 ggctggaacc gctggacggc ctgctttcgc gagggcaccc tttcccgaat gagagttggc
     3301 tgcggagtgc gaactagctg gatgacgcat cccgtgcgcc gcagcccctg agccccgttg
     3361 cgctgacttg ccgtgagcgc gatgggccgg agcggaaacc gcctttgggt gtgcggggtt
     3421 aacagaagtt cttggcgcgt gcgtcgcggg cgcgttacgc ccggcggcag cccgctggtg
     3481 tgcaccacca gcacctggag catgagccgc cgcttcagcc gacccaatcc ccaacaacac
     3541 cacagacacc attaccgcga gcccagtcgc aatagaatga agccgcatgc aagtgcttag
     3601 cacgctcacc acctcgagtc gtttcaaatt cgatgattcg agtcgtatcg agtggagttc
     3661 caagtggtct gtaagcgacc ttcgtagatg tttcgagaca tcgattactc gatgaatcat
     3721 cagctcgatt ccaacatgcg aattagcgtc gagcttgcgc tcgagacgaa gtgtttcgct
     3781 cgcacttcga agctcgccgc cgaccagatt gggctgagcc gcagtcgcga ggttccaggc
     3841 gtcccgtttc gacccacgcg accgtcgcgc cgcgcgttac gcacgcgaag ttcggcctgc
     3901 gccctctctc ttcattgaat ttcggtatcg aattgtcact cgaagctcgc cgccgccgcc
     3961 gtaatgctgc tcccgaggct caactcagcc tgccgtgggc ggaacgaaaa gtcgcccctg
     4021 gcccggggcc acaggcctcg ggtttgcaca tccatcgcag caaccgctta gagacactgg
     4081 tggcaagcct gggagcgctg ctcgagcggc ccaaaggggg cccgctcgat cccgagatca
     4141 tcgctgcgcc agggcagggc atggccgtct ggctatctat gcagctctcg aagcaactgg
     4201 gggtatgggc cgctccaatt cgttttccac gtgagctcgt cgatcatttg atgttgcgcg
     4261 tgctgggtga ggacgcgcgc gagttgagcg ccgtctctcg agatgcgatg gggtttgcga
     4321 ttcaagccga gctcccgagt ttgttgaatg agccggggtt cgacggactc cggcgctacc
     4381 tcgagcaaga cgagtacggg gtgcgacgtg ccgagctcgc gatgcggctc gccgcaacat
     4441 tcgatcaata cctcacctat cgcagtgact gggtacgtgg cttcgaggag ggcgcgttgc
     4501 tggatttacc agccggaaac caatttcaga cgctgctgtt tcggagagtc gtgggccgtc
     4561 tgagacgtaa gcacctggct cagctcgaac cgcggctgtt ggatgtcttg tccggcgcca
     4621 ccgcgcccgc cagcctgccc gcgcgaattt cgatatttgg catctccacg ttgccacccc
     4681 tgttcgtgcg catcctggcg caagttgctc gtcacatcga agtgcacttc tttctatttt
     4741 cgcccggtgc gcacgcctgg tcaccggaag cgggaacgcg ctcacgacat ccattgctcg
     4801 agcgtttggg cgccgtcggt gcggacttcg atggcgtgct ctcggcctgt gcctcggatg
     4861 ccgacctgac cgcgattcga cacgagcact tcgtcattcc ggagcccaat cagctgctaa
     4921 cggcgttgca ggcagacttg gccggggatc ctcgacctgc tgctccagag ctacggctcg
     4981 aggcctgcac ggccacgagc tccatcgcgg tgcactcctg tcatggcccg atgcgcgagg
     5041 tcgaggttct gcacgatcag ctcctgtcat tgctgactcg cgctcgagat ccggtgcggc
     5101 ccgaagaggt cgtggtgatg gtgtccgacc tggatacgta cgctcccctc atcgacgccg
     5161 tgttcgcgcg tgagcccggg catccggagt tcattcctta cgtggtttcg gacacgctgt
     5221 tgtcgttgga tgcctccaca ctgcgtgcct tcgatcgcat gctgggcatg gtgcgcggcc
     5281 gcgtcaccgc gtcggaagtg ctcgacattc tcacggtgcc cgcagtgctg gcgcgcttcg
     5341 acctcgactt gctggaggcg gatcgactgc gagattgggt atgcgaaagt ggcgtgcgct
     5401 gggggatgga tgccgagcac cgggagcgca tgggcgtacc cgcctccgac aacaacacgt
     5461 ttcggttggg catgcagcgg ctgatgctcg gttacgcgct gcctacttct ggcgaggcct
     5521 tgttcgaggg ggtgctgccc tatgagcaga tcgagggaca ggatctggtg ctgctcggca
     5581 aattcagcag ctttcgtcga cggctcttcc actggctccg agaattggag cgcccgaggg
     5641 ccatggcggc atgggtggag accctgaccc agctgctcgg cgacctgttc tccgagtcag
     5701 tcgagtcggc ggcgcgcctg tcccgcatcc gaaaggggct cgccgatctg gccgccgctt
     5761 ctctgaccgc gcgcttcggc gatgcgctcg agatcgaagt cgtgcacaag ttgctcgggc
     5821 agaagctcaa ggcgatcgca ccagagcacg cctacctcag tgagggggtc acgttctgcg
     5881 caatgctgcc gatgcgcaac gtcccgttcc gcgtggtgtg tgcgctcggc atgaacgaca
     5941 atacctttcc gcgaactccg cccatcagtg agttcgattt gatgcagagc aattatcagc
     6001 cgctgaggcc gggagaccga tcgctccggg aagacgatcg gtacttgttc ctggaggcct
     6061 tgtgccaagc ccgtgaacgg gtgttgctct tctatactgg gcgcagcgtg cgcgataaca
     6121 agcctcgtcc cccgtcacgt tgcgtgtcgg agctcctgga aagtatcgag cgacgcttgg
     6181 gtgttccggc ccgggacgtc gtcgtagagc accggctgca gggtttcagt cgggcgtatt
     6241 tcgatgcccg cgattcgcgc ctctttagct actccaagag ttacttgaac gcggcccgca
     6301 gcatggcgag ggcccagtcc tcgctggatc cattcgtcgg taagccactc gaagcggagc
     6361 ttcccgatgc acttcgcctc gaagatctgg tgcgtttctt ccgctcgccg ccggcctatt
     6421 acttgaaccg ccgcctgggc atctatctcg acgaagagga catcgacgtt tcggatcgcg
     6481 agcctctgga gctctccgcg ttggacaatt ggcgactcgg ctcttcgctg atcgaacacc
     6541 tgctcaaggg ccgctctttg caggaggccg ccgatcttct gcaggccagt ttggagttgc
     6601 cgcatggttc ttggggtacc g
//
LOCUS       DASTKU010000171         8615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_923726, whole genome shotgun sequence.
ACCESSION   DASTKU010000171 DASTKU010000000
VERSION     DASTKU010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8615
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923726"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFQ61_06140"
     CDS             <1..523
                     /locus_tag="VFQ61_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9954061.1"
                     /translation="SALRRSCYALPVVLMSAFMDRSAVERAEAAGALDILSKPLDLNR
                     LFNLAEEFFSPKHPVLVVEDNKALLENVTEALRDSGLVPLVGHDAESALAHRCLPRVA
                     LVDLRLPDQNGVLVARRLFARNPKMRIFYITGSEELSRELLKVPDSRTELVLKPFEMS
                     DLLERVNAAMAVA"
     gene            520..1938
                     /locus_tag="VFQ61_06145"
     CDS             520..1938
                     /locus_tag="VFQ61_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9954062.1"
                     /translation="MTGSAARRIHALIVDDNEELAENVADILAGIEGMELETHLAASA
                     HEAIRQVERLGDELDLSLVDLRLPDGNGLGLLSQLRERSSFAEFLVITGDLSIESAVA
                     AVDARVFAYVLKPFRPLELQSTARHAITQVELKRRARRLQRELELSEHRHREVVEAIP
                     AFVLALDREHQIVLWNQRLEAVTGFSRAEMIGQSGADLIREGGDRKLDLKGGGHRLVR
                     WQCTEVANTTYALGIDVTEEREMLRRTLQAERLASAGTLAAGLAHEVRNPLNSATLQL
                     QVLKRRIERGNVEKDKLLPVISVVDGEIQRLTHLVSDFLAFARPHKLEIESTDLNDFL
                     NEVLDLVTPECKLANVQVCKDFAQGIGRLELEPNRIRQVLLNLIRNAIEAMPDGGLLT
                     LHTSEADSAGFVHIEVQDPGKGFAEDAPVFDAFYTTKPGGTGLGLAIAHRIVTEHGGV
                     LKAEPRSPGACFSVLLPQHSKT"
     gene            1935..3002
                     /locus_tag="VFQ61_06150"
     CDS             1935..3002
                     /locus_tag="VFQ61_06150"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET9954063.1"
                     /translation="MSSGPDHPPCAPVEPEAAIDASTLRTAVELLNQGRLVAFPTETV
                     YGLGADAENPVALRRIFEAKGRPSSHPLILHVAHFDQVRELVTALPPEAELLARLFWP
                     GPLTLVLKKSSRVLSALTGGQDTVAVRVPGHAVALALLQAFGRGIAAPSANRFGAISP
                     TTAQHVRADLGARVDLVLDGGACRVGLESTILDLSRGAPVILRPGGITAEALQNALGR
                     SVNQERASSIRAPGQLASHYAPSAELLMVAASELGPRAEALQQRGARVAVLHRDDMLP
                     PRVARTLAVPHQIEGFAHTLYAALRELDQPDVDVILAVAPEGQGLGEAVLDRLTRAAA
                     PRLRGEETPKSSPIDRVSLLD"
     gene            3095..4528
                     /locus_tag="VFQ61_06155"
     CDS             3095..4528
                     /locus_tag="VFQ61_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9954064.1"
                     /translation="MSGEPTSRPSGSRGRILVVDDEANARSALAEILREEGYSVETAS
                     DGFKALAKAEEFDPDLVLTDLKMPGMDGVELLRRLLRAPESRVAVVVMTAFGAVETAV
                     SAMREGAVDYLTKPLNTDELLLVLDRALENIRLRREASMLRSQLRERYRFENIIGASA
                     EMQQVFKSVAQVAQSRATVLISGESGTGKELVAAALHHRSPRATGPFVRLHCAALAET
                     LLESELFGHERGAFTGADRRREGRFEQANGGTLFLDEIGDISPSTQVKLLRALQEKEF
                     ERVGSNQTLHVDVRVVTATNRDLKEMVAAGKFREDLYYRLNVINIQIPPLRRRPSDIP
                     ALVEHFLKRYAAENQKPVTRVSDAALALLVGYSWPGNVRELENVIERAVVLTEGESIE
                     PYHLPGELAATGRGSSVPIVPGATMEELERHAILKTLESVGGSTSKAAEILGISVRKI
                     QYKLQEYGAAPKSQTPAVQQERPSSDD"
     gene            complement(4498..5547)
                     /locus_tag="VFQ61_06160"
     CDS             complement(4498..5547)
                     /locus_tag="VFQ61_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET9954065.1"
                     /translation="MPEAAPSQAAALVKQAPPRDSGASRRSEAPGGELRNARVRRVLW
                     LTMGLNLVVAILKIAYGHWSHALSIRADGFHSLTDASNNIVGLLGVYLAARPPDRDHP
                     YGHAKFEILAAGVVGLSLLAMAYDVVRNAFERALGSGEAPDIDARVFVVLVLTLGVNL
                     WVSSYERRMGERYESSFLLSDATHTRSDVLVTLGVMLTAALVELGYPNLDIAAAFVIA
                     AFIAWAGISVLRHNLAYLADHKAVDDEVVSAIVLRVPGVASTHKIRTRGSPGAIHVDL
                     HIQIARHLDVVTAHQVTHWVIEAIQSGVQGVQDVVVHTEPAAVGQPYNPLPDANAAST
                     ERAGPPQSSDEGRSC"
     gene            5731..6231
                     /locus_tag="VFQ61_06165"
     CDS             5731..6231
                     /locus_tag="VFQ61_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9954066.1"
                     /translation="MLRLESILVPVDFSACSRRALELARPFAAGARVEVLHVAEIPQF
                     VPQNALLFGMTEPAQPGLVAPPPLTTADSRPFGELVRARATERLELFVNEARASGIPI
                     TSSKVVLGVASEVILETILGGDYDLVVLGTHGRRGISQWLLGSVAEKIVRHSTIPVLT
                     VRVPGD"
     gene            complement(6268..7695)
                     /locus_tag="VFQ61_06170"
     CDS             complement(6268..7695)
                     /locus_tag="VFQ61_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9954067.1"
                     /translation="MSQLFELRRILVPCLGLGCWLYGARPSFAQSAAPAGPPAAMQPA
                     PAAQAASPSQSLPAQQPNGTRPGSQSAPGNTVAPPSSQAAVATAVEAPPQLQLPSLER
                     VVALAMERAPEVTMGKATVEASRSSLVGARVAPLLNPYLEVVAEQGRGVNRNVAVTGS
                     LFLPLEVAGQRGRRVAEAESFIHWNQATLGRVRARAAGEAVSVFGQLLAVAARLETLQ
                     ELVQSAGTEAQVLAARRDAGDATERDAQLAAVERARIAVQLEESRAALHAAQGELLRL
                     TGQRFVVPSGTHVFPRLAPRPMQSKDAAPWVQAAEAEASYYARTHERLGRDRVGPVSV
                     ILQGGRGDSGETRLGAGLAWELPTFRRLQGERARALSEAARAQVEARVLRAGIEQRLS
                     RISEEYAGLMAAIQVLDREAIPAAQAATESATRMQRAGKTDLLSVVVARRDLYVLRLR
                     RLEIAERNWELLGEWVELTGRLPNQ"
     gene            complement(7717..>8615)
                     /locus_tag="VFQ61_06175"
     CDS             complement(7717..>8615)
                     /locus_tag="VFQ61_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9954068.1"
                     /translation="GSMFEGERKFDLRVVSPPTQPTAEALGDLFVRTARGGSIPLREV
                     LVLDEGDGPTAIRREDRRRALRVDVNLRGRDLVSWVAEAQQKVKDEVSLPAGYEIKWG
                     GQFENFQRAQGRLMLMLPVVVTIIFGMLLWMFRDFRFAGAVFALVPLLLTGGMIGLLL
                     SGLSFSLPAAVGFIALGGIGVLNGVVVASEVRHRMDEGEPLDSAIVHGSAHVMRAVLT
                     TAAVAALGFLPMAIASGAGAEVQRPLARVVVFGMMFGTVLTLTVLPGILRFALKNYEA
                     PEDADELANEGLPEMARPATAG"
BASE COUNT         1547 a         2816 c         2769 g         1483 t
ORIGIN      
        1 ttcggcgctg cgtcggagtt gttacgcgct gccagtggtg ctgatgagcg ccttcatgga
       61 tcgttcggcg gtcgagcggg cggaagcggc cggcgcgctc gatattctta gtaagcccct
      121 cgacctaaac cgtcttttca atctggccga agagttcttc tcccctaagc accccgtgct
      181 ggtggtggaa gacaacaagg ccttgctcga gaacgtaacc gaggctctcc gggacagcgg
      241 gctcgtgccg ctagtgggcc atgacgctga gtcggcgctc gcgcaccgct gcctgccccg
      301 cgtggcgctg gtagatctgc gcttgccgga tcagaacgga gttctggtcg cgcgccgctt
      361 gtttgctcgg aaccccaaga tgcgaatctt ctacatcacg ggcagcgagg agctcagtcg
      421 ggagctactc aaggtgccgg actcccggac cgagctcgtg ctcaagccgt tcgaaatgtc
      481 ggacctgctc gagcgagtga atgcggcgat ggcggtggcg tgactggcag cgctgcgcga
      541 cgtatccacg cgctgatcgt cgatgacaac gaggagctcg ccgagaatgt cgctgacatc
      601 cttgcgggca tcgaaggcat ggagctcgag acccatctcg ctgcttccgc ccacgaggcg
      661 atccggcagg tagagcgcct gggagacgaa ctcgatctct cactcgtgga tctcaggctg
      721 ccggacggta acgggctggg gctgctgagc caactccgcg aacgctcgag cttcgcggag
      781 ttcctggtga tcacgggcga cctctcgatc gagagcgcgg ttgcggcagt ggacgcgcga
      841 gtgttcgcct acgtgctcaa gccgttccgc ccgctggagc tgcagtcgac ggcgcggcat
      901 gcgattaccc aagtcgagct gaagcggcgc gcccggcgtc tgcagcggga attggagctc
      961 agcgagcacc gccaccgcga ggtagtagaa gccatccccg ccttcgtttt ggccctcgat
     1021 cgagagcacc aaatcgtgct gtggaaccag cggctcgaag ccgtgacggg cttctcgcgg
     1081 gcagagatga ttggccaatc aggcgcggat ctgatccgcg agggtggcga tcgaaagctc
     1141 gacttgaaag gcggcggcca ccgcttggtg cgctggcaat gcaccgaggt ggcgaacacc
     1201 acctatgccc tcggcatcga cgtcaccgag gagcgcgaga tgctgcgccg gacgttgcaa
     1261 gccgagcgtt tggcgtccgc gggcacgctg gctgccggcc tcgcgcacga ggtcagaaac
     1321 ccgctcaact cagcgaccct tcagctgcag gtgctaaagc ggcgcatcga gcgcggcaac
     1381 gtcgaaaagg acaagctgct accggtgatc tcggtggtgg atggtgagat tcagcgcctg
     1441 acccatttgg tcagcgattt cctagctttc gcgcggccgc acaagctaga aatcgagagc
     1501 accgatctga atgacttctt gaacgaggtg ctcgatttgg tcaccccgga gtgcaagctc
     1561 gctaacgtgc aggtctgcaa ggacttcgcg caaggcatcg ggcggctaga gctcgagccg
     1621 aaccgaattc gtcaggtgtt gctcaatttg attcgtaacg ccatcgaagc catgcctgac
     1681 ggtggtttgc tcaccctcca cacgagtgag gcagactcgg ccggattcgt gcacatcgag
     1741 gtccaagatc ctggcaaagg cttcgcggaa gacgcgccag tgttcgacgc tttctacacc
     1801 accaagccgg gaggaacggg cctggggctc gccatcgcgc accgcatcgt gactgaacac
     1861 ggcggagtac tgaaagcgga gccgcgctct cctggggcgt gtttttccgt gctactgccc
     1921 cagcacagca agacatgagc tctggtcccg atcaccctcc ctgcgcgccc gtcgaacccg
     1981 aagccgcgat cgacgcaagt accctgcgca ccgcagtcga gctcctgaat caggggcggc
     2041 tggtcgcgtt tccgaccgag actgtgtacg ggcttggagc ggatgccgaa aaccccgtcg
     2101 cgctgaggcg gatcttcgag gccaaggggc gcccctcgag ccatccgctg attctgcatg
     2161 tggctcattt cgaccaggtt cgggagctcg tcacggctct cccaccggaa gcggagctct
     2221 tggcccggtt gttctggccg ggtccgctca cgttggtgct gaagaagagc agccgcgtgc
     2281 tgagtgcgtt gaccggcggt caagatacgg tggctgtacg cgtgccgggg cacgccgtag
     2341 cgctcgcgct gctgcaagcc ttcgggcgcg gtattgccgc cccttccgcc aaccgcttcg
     2401 gcgcgatcag tcctacgacc gcccagcacg tgcgcgcgga tcttggtgcg cgagtcgact
     2461 tggtgctcga cggaggggcg tgccgggtcg ggctcgagtc gactattttg gacctttcac
     2521 gcggggcgcc cgtgatcttg cgtccgggtg gcatcacggc ggaggccctg caaaatgcgc
     2581 tgggccgcag cgtcaaccag gagcgcgcct cctcgatccg cgcgcctggc caactcgcct
     2641 cgcactacgc acccagcgcc gagttgctaa tggtggccgc gagcgagctc gggccccgcg
     2701 ccgaagcatt gcagcaacgg ggggcgcgcg tcgcggtgct ccaccgagac gatatgctgc
     2761 cgccccgcgt cgcacgtaca ctcgcggttc cgcaccagat cgagggcttc gcgcacacgc
     2821 tgtacgccgc gttgagggaa ctcgatcagc cggatgtcga cgtgattctc gcggtcgccc
     2881 cggagggcca gggtctcggg gaagccgtgc tcgaccggct cactcgtgcg gcggcgcccc
     2941 gacttcgagg cgaggaaacc ccgaagagca gcccaatcga tagggtttct ttgctagact
     3001 gacgcccgat gttggcgtcc gtactaccga cgtttgaaat ttcgccgccc ggagaacctg
     3061 ggcacgaagc tcccaccttc gaggcttcga acccatgagc ggggagccga cgtcccgccc
     3121 gagtggcagc cgagggcgca ttctcgtcgt cgacgacgag gccaacgccc gcagcgcctt
     3181 ggcggaaatc ctgcgcgagg agggctattc ggtcgagacc gcctcggatg gtttcaaagc
     3241 gctcgccaaa gcggaagagt tcgatccaga tctcgtgctg accgacctca agatgccggg
     3301 gatggatggg gtggagttgt tgcggcgttt actgcgcgcg cccgagagcc gcgtggcggt
     3361 cgtcgtgatg actgcgttcg gcgcggtgga gaccgccgtg tccgccatgc gcgagggcgc
     3421 cgtcgattat ttgaccaagc cgctcaacac cgacgagctc ctgttggtcc tcgaccgggc
     3481 cctggagaat atccggttac ggcgcgaagc cagcatgctg cgctcgcaac tgcgcgagcg
     3541 ctatcgcttc gagaacatca tcggcgcatc ggcggagatg cagcaggtgt tcaagagcgt
     3601 ggcgcaggtc gcccagagcc gggcgacagt gctcatctcg ggcgaatccg gcaccggaaa
     3661 agagctcgtg gcggcggctc tgcatcaccg ctcgccacgc gccacggggc cgttcgtgcg
     3721 cttgcattgc gcggccttgg ccgagacact gctcgagagc gagctcttcg gccatgaacg
     3781 aggcgcgttc acgggcgccg atcgacggcg cgaaggtcgc ttcgaacaag ccaatggcgg
     3841 cacgctgttc ttggacgaaa tcggcgatat ttcgccctcg acccaggtca agctgctgcg
     3901 ggccttgcaa gagaaggagt tcgagcgggt cggaagcaat cagacgctac acgtggacgt
     3961 gcgcgtcgtg accgccacga accgcgatct caaggagatg gtggcggccg gaaagttccg
     4021 tgaggacctc tactatcgcc tcaacgtcat caatatccag attcctccgc tgcggcgtcg
     4081 ccccagcgat attccggcct tggtcgagca cttcctcaag cgctacgcgg ccgagaacca
     4141 gaaaccagtc acgcgagtga gtgacgcggc tctagcgttg ctcgtagggt attcctggcc
     4201 gggcaacgtg cgcgagctcg agaacgtgat cgagcgcgcg gtggtgctga ccgagggcga
     4261 gagtatcgag ccgtaccacc taccgggtga attggcggcg accggccgcg gcagttcggt
     4321 gccaatcgtc cccggggcga ccatggaaga gctcgagcgc cacgccatcc tgaagacgct
     4381 ggaatcggta ggcggctcga ccagcaaagc cgcggagatt ttgggcatca gcgtacgaaa
     4441 gatccaatac aagctgcaag agtacggggc cgctccgaag agccaaactc ctgccgttca
     4501 gcaggagcgc ccttcgtccg acgattgagg cggcccggcg cgctcggtac tcgcggcgtt
     4561 cgcgtccggc agagggttgt aaggctggcc caccgcagcg ggttcggtgt gcacgaccac
     4621 gtcttgcaca ccttgcacgc cgctttggat tgcttcgatc acccagtgtg tcacctgatg
     4681 cgcggtcacg acgtcgaggt gccgggcgat ttggatgtgc aagtccacgt ggattgcgcc
     4741 gggtgagccg cgagttcgga tcttgtgcgt gctcgcgacc cctggcacgc ggagcacaat
     4801 cgcggacacc acctcgtcat ccaccgcctt gtgatcggcc aagtaagcca gattgtgccg
     4861 caagacgctg atccctgccc acgcgatgaa agcggcgatg acgaaggcgg ccgctatatc
     4921 caagttcggg tatccgagct ccacgagcgc cgccgtcaac atcactccca acgtcacgag
     4981 cacgtccgag cgcgtatggg tggcgtcgct cagcaagaag ctgctctcgt agcgttcccc
     5041 catgcggcgc tcgtaactcg agacccacag gtttacgccg agcgtgagca cgagaacgac
     5101 gaatacccgc gcatcgatgt cgggggcctc gccgctcccc aaagcccgct cgaacgcgtt
     5161 ccgcacgacg tcgtaagcca tggcgagcaa agaaagcccc accacccctg ccgccaaaat
     5221 ttcgaacttc gcgtgcccgt aggggtggtc gcgatcgggc gggcgagctg ccaagtacac
     5281 gcccagcaat ccgacgatgt tgttggatgc gtccgtcagc gaatgaaaac cgtcggcccg
     5341 aatcgaaagc gcgtggctcc agtgcccgta ggcaatcttc agaattgcca cgaccagatt
     5401 caagcccatg gtcagccaaa gcacccggcg aacgcgagca tttctaagct cccccccggg
     5461 ggcctcgctg cgacgtgaag cgcctgaatc cctcggcggg gcttgcttca cgagcgcagc
     5521 ggcttgggac ggcgcggctt cgggcatgga tccggcgcaa aggttgcgct gttcgaggca
     5581 cccccgcaat cggtccgcgt tcgattcgcc ggctgcactg cgaaacgtct cctcatgcaa
     5641 ttttttctat gcggtggggt ggcgtgcgtc ttgcagccac gtcgacaggc gagccaccat
     5701 cactcgttgg gaacttcagg gagacaagcc atgctccgac tcgagtccat cctcgttccg
     5761 gtcgacttct ccgcctgctc gcgccgcgcg ctcgaactcg ctaggccatt tgcggccggt
     5821 gctcgagtgg aggtgctgca cgtggccgaa atcccgcagt tcgtccccca aaacgcgttg
     5881 ttgttcggga tgaccgaacc tgctcagccg ggtctggtgg cgccgcctcc cctgacgacc
     5941 gcggactcgc gaccgttcgg tgagttggtg cgcgcacggg cgacagagag actcgagctt
     6001 ttcgtgaatg aggcccgcgc ctccgggatc ccaatcacct cctccaaggt agtgttgggc
     6061 gtcgcctccg aggtgattct ggagacgatc ctaggtgggg attacgatct cgtggtgctc
     6121 ggaacgcacg gcagacgcgg gattagccaa tggctactcg gcagcgtggc ggagaagatc
     6181 gtcaggcact cgacgatccc cgtcttgacc gtgcgggtgc ccggagactg agcggcgctc
     6241 gatcgcgccc cgttgagttg gttcgaacta ctggtttggg agccgtccgg tgagctcgac
     6301 ccattcgccg agcagctccc agtttctctc tgcgatttcc agccgacgca gccgcagaac
     6361 atagaggtcg cgtcgagcaa ccactacgct cagcaggtcg gtcttgcccg cccgctgcat
     6421 gcgcgtggcg gactctgtgg ccgcctgtgc cgccgggatg gcctctcgat ccaacacctg
     6481 gattgccgcc atgagacccg cgtactcctc ggaaattcgg gataggcgct gctcgatccc
     6541 ggctcgcagc acgcgtgcct cgacctgcgc tcgtgccgct tcggagagcg cccgtgcccg
     6601 ctcaccttgc agtcgacgga acgtcggtag ttcccaagcc aagcccgccc ccaagcgcgt
     6661 ctcaccggaa tcgccgcgcc ctccctggag gatcacgctg accggcccga cccggtcccg
     6721 ccccaagcgc tcgtgggttc gcgcgtaata gctggcttct gcttcagcgg cttgcaccca
     6781 cggtgccgcg tccttcgact gcatgggacg cggcgcgagg cgcgggaaaa cgtgcgtgcc
     6841 actgggcact acgaagcgct gccccgtcaa gcgcagcaac tcgccctgcg ccgcatgcaa
     6901 agcggcgcgc gattcctcca attggacggc gatgcgcgca cgttccacgg ccgcgagctg
     6961 cgcatcccgc tcggtcgcgt cccctgcgtc gcgtcgcgct gccaagacct gcgcctcggt
     7021 tcccgcagac tgcacgagct cttgcaatgt ctcgaggcgc gccgcgaccg caagcagctg
     7081 cccgaagacg ctcaccgctt cacccgcggc gcgcgcgcgc acacgcccca gagtggcctg
     7141 gttccagtga atgaaagact ccgcctcggc cacgcggcga ccgcgctgac ccgcgacttc
     7201 gagcggtagg aacagtgaac cggtcacggc tacattgcgg ttcaccccgc ggccttgctc
     7261 ggcaaccact tccaagtagg gattcaggag cggagccacg cgagcaccca cgaggctcga
     7321 gcggcttgct tcgaccgtag ctttgcccat cgtaacctcg ggcgcccgtt ccatggccag
     7381 ggcaacgact cgctcgagcg acggcagctg tagctgcggt ggcgcttcta ccgcggtcgc
     7441 gaccgcagct tgcgaagacg gtggagctac ggtgttgccg ggagcgctct gggatcccgg
     7501 ccgtgtgccg ttgggttgct gcgcaggcag cgactgactt gggctcgccg cttgcgcggc
     7561 gggtgcgggc tgcatcgccg cgggagggcc cgccggagcc gcggactggg caaaactagg
     7621 ccgagccccg tacaaccaac aaccaagtcc caagcagggg accaagatac gccgtaattc
     7681 gaagagttgg ctcatgatta tccagaacgt cgctgattat ccggcggtgg cgggacgagc
     7741 catttctggc aagccttcgt ttgcgagctc gtctgcgtct tcgggcgcct cgtagttctt
     7801 cagggcgaag cggagaatgc caggcagaac cgtcaacgtc agcacggtcc cgaacatcat
     7861 gccgaacacg acgacacgag ccaacgggcg ctgcacctcc gcgccggccc cggacgcgat
     7921 cgccatcggc agaaagccca gtgccgcgac cgccgcggtc gtgagcacgg ctcgcatcac
     7981 gtgagccgat ccgtggacga ttgcggaatc caacggttca ccttcgtcca tcctgtgccg
     8041 cacttcgctc gccacgacta cgccattgag cacgccgata ccaccaagcg cgatgaagcc
     8101 aactgccgcc ggcaagctga acgagagacc gctcagtagc agaccaatca tgcctcccgt
     8161 gagcagcaag ggcaccaaag cgaagacggc gcccgcgaag cggaagtcgc ggaacatcca
     8221 gagcagcatg ccgaaaatga tggtgacgac cacgggcaac atcagcatca agcgcccctg
     8281 cgcgcgctgg aagttctcga actgaccacc ccatttgatc tcgtagcctg cgggtagaga
     8341 gacttcgtcc ttgaccttct gttgggcctc agcaacccat gacacgagat ctcgaccccg
     8401 gagattcaca tccacgcgta aggcccgtcg acgatcctcg cgccgaatcg cggtggggcc
     8461 gtcaccctcg tcgagcacga gcacctcacg caacgggatg ctaccgccgc gcgcggtgcg
     8521 cacgaacaga tctcccaaag cctcggccgt cggctgggtg ggcggagaaa ccacgcgcag
     8581 gtcgaacttg cgctcgccct cgaacatgct gccca
//
LOCUS       DASTKU010000172         7280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1816995, whole genome shotgun sequence.
ACCESSION   DASTKU010000172 DASTKU010000000
VERSION     DASTKU010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816995"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1196
                     /locus_tag="VFQ61_06180"
     CDS             279..1196
                     /locus_tag="VFQ61_06180"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9954069.1"
                     /translation="MEVIRMDPVSAARTARESLARGLSALQTPGIPPALMAVADPVAQ
                     AMSALHRIEASGGGALAEAAPYALACVRQALGLLQSPGVSHPAVDSAMEAIAGSLGVV
                     HQLSVSAAPAPAPVSPYAGTQMMDQSLFQAPAVAAPRAGNVAPAQGYPPAPVYGAPPR
                     PQAPPPAAPVAQAAPPAAVAAQAPAAGLEAFPPGLPRVEAALGAHSPTNFYKGLSSND
                     IIESGGIFIATYKIPKVGSQVVVHLSLPGGYEFDALAIVRWAREAPDASAGQAPPGFG
                     AQFVQISPEGRQLVYRYVRNREPLFHDDL"
     gene            1203..4253
                     /locus_tag="VFQ61_06185"
     CDS             1203..4253
                     /locus_tag="VFQ61_06185"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9954070.1"
                     /translation="MTAGLGSSRRALRSEGGRVLSNVKRWWTRSALPARAWLVLFVLL
                     ASIGVPRPARAGDPYLRFRTIHTPHFRVHYHGGLERLARRAAQQAEAIHEALVPDLGW
                     APSEPTQLVLADDSDFANGYGSGVPYNAIRLHVSAPDDMSSLQDYDDWLANMITHEYT
                     HILHIDNLSGIPALLNTLFGRVSVPNQSQPRWILEGFAVAMESAHSSSGRLRSTLFDM
                     YLRADALEDNLAALDEISGPTRRWPGGELWYLYGAKFVEWITETYGPDTLARIASEYG
                     SMPIPFAINRAVRRVTSRTYPELYAGFQSSIAERTREFVAGVRARGLREGRRLTFGQR
                     ISRAPRFVPAACRETASPQVSYYRDDSLGKPGLYRFLLGSHGVASPELIARSSGGAHS
                     YTPECQIVFDSLAPSARYYQFLDLFRVPPRAHDMSRFDLRRERLTVGARAREPDVSPD
                     GRQLTYVTNDAGTTTLKLAELTARGIERERVLVPSAEFEQAYTPRFSPDGRRIAYSAW
                     TAGGFRDIRVVDVASGRFFELWHDRAIDQQPTWSRDGSTLYFVSDRTGAANVYAYELA
                     SGRLWQVTNVLTGAYMPEISDDGRTLLYVGYTSGGFDVFALELDPSKFLEPIPSVARA
                     AHPIAHRARALDARGQRPFADQPYDPLPTLRPRAYRLEYGPGTFGDAITVKTRGSDVL
                     DYHTILASVILEAREPDWRGSLDYTYDRLPFSLHLSAFHDAAPSYVYRFEEPRERHVD
                     YSNGVTSGIGWSIPGEYDTQTLGFSFTTAGITRAEAVPKPREPYDPLPRTTSVGLYAG
                     ARLAYSYGNAEGSLHGVSAERGFNLFLALDVADPAFGSDWRLAAFTGALTTYFSMPWA
                     RHHVLALALSGGGSSGSYPRSYFHTGGFVDHNAFDVFRTGFRQSGFVLRGYEPGQFIG
                     SEYTLLNVEYRFPLLYADRGLSTLPLFLQQVSGTLFADYGGAYEQVDPKDPLRVMHLG
                     LGGELWLELVLGYRYSGSLRLGVARGTGEMAHGVQSYFVAAAAF"
     gene            4327..4839
                     /locus_tag="VFQ61_06190"
     CDS             4327..4839
                     /locus_tag="VFQ61_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954071.1"
                     /translation="MRAIRSFATVSLSTALTGGVLLGGCVLPPSASERAADAAREVNV
                     ASRFGRMDRAMELTSTGLRSSFLQHRAAWGKDIRIFDVELVALQLPSKDRALAEVEYS
                     WTRTRETELRSTRVAQEFHDPGGGFRLVRERRIAGDKGLFAEPGAGPSSSAKASGDVQ
                     FATKVIPPSE"
     gene            4877..5383
                     /locus_tag="VFQ61_06195"
     CDS             4877..5383
                     /locus_tag="VFQ61_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954072.1"
                     /translation="MAIAPSTKGVALGDERRADPRNTTEDDVQRFLDGFFFERSARTL
                     SARRAKRAFLERWALRRRLFCEWRRSNFPIEKLSCWQREREAHPRQRISALWVATKAG
                     TARLGLSLAPAEAQIELGLAHCLLFGEQHATAEPSRKTRRHSARQWVQVGTHSCVTTT
                     AQLKPSAV"
     gene            complement(5445..5609)
                     /locus_tag="VFQ61_06200"
     CDS             complement(5445..5609)
                     /locus_tag="VFQ61_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954073.1"
                     /translation="MIEFSDGMHEYVTTPVKRVLRLAARDVFYIETVNSRYRLEVRDR
                     ERALEDVSSG"
     gene            complement(6356..>7280)
                     /locus_tag="VFQ61_06205"
     CDS             complement(6356..>7280)
                     /locus_tag="VFQ61_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9954074.1"
                     /translation="VKTGKVLAYASYVAEGEPFDVNARAEAPAASVFKVVTGAALVER
                     AGLNAETEQCYHGGKSRISQDELQDNPQRDRWCATIGIAMGRSLNVVFGRLAQKHLTP
                     EDLTAMGGALGFGAPVPFVVPNQAPKLQIPAEPVEFARTSAGFWNTTLSPLAGAVLAQ
                     TIANGGVALQPRVVSSILKGRDIVWRDELEPRVLRRALKPSTAAELTQMMTQTVSNGS
                     AFKAFHDERGSAYLPGIDVAGKTGTLSDHEANRHYTWFIGFAPADKPEVAISTLVVNT
                     PTWQIKAPQLARDVLRAYFARHGAKGVTPPS"
BASE COUNT         1240 a         2338 c         2291 g         1411 t
ORIGIN      
        1 gtgatagcgc actactggtc gagcgcgcgg ttttgtctgc gccggaagac gacgacgagg
       61 ggccgggcga tagcgaagac ctcgcggaaa gcacgctgga cgccacccaa ctaggagagc
      121 atctgcggat caggctcgag agccggtctg ctccgtccga ctttcgggtg cggtaggcag
      181 aaagagcgcc tcacgaccgc cgttctgcgc gcgacgtcgc cccaaagcag tggttggaaa
      241 gcaggggccg aatgatagac tgctccagtc cgtgaatcgt ggaggtcatt cgtatggatc
      301 cggtgagcgc ggcgcggacc gcgcgtgaaa gtctagcgcg aggcctttcg gcacttcaaa
      361 cccccggcat tccgccggca ctcatggcag tggccgatcc ggttgctcaa gccatgagcg
      421 cattgcatcg catcgaagcg agcggcgggg gcgccttggc agaggctgcg ccttacgcgc
      481 tggcttgtgt tcgccaagca ctcggcttgc tgcaatcgcc cggcgtttcc catcctgcgg
      541 tggattcggc catggaagcg atcgccggat cactcggtgt cgtgcaccag ctctccgtga
      601 gtgccgcgcc agctccggcc cccgtctcgc cgtacgcggg aacgcagatg atggatcagt
      661 cgctattcca ggcacccgct gtggccgctc cccgagccgg caacgtggcg cccgctcagg
      721 gatacccacc tgctccggtt tatggtgcgc caccgcgtcc gcaagcgccg ccaccagcgg
      781 caccggttgc gcaggcggcg ccaccagcgg ccgttgcggc tcaggcacct gcggcgggtc
      841 tcgaagcgtt cccgcctgga ctaccccgag tcgaagcggc gctcggagca cacagcccaa
      901 ccaatttcta caagggcttg agcagcaacg acatcatcga atccggcggc atcttcatcg
      961 cgacgtacaa gatccccaag gttggttcgc aagtcgtggt gcacctctcg cttcccggcg
     1021 gctacgagtt cgacgctttg gccatcgtgc gttgggcacg tgaagcgccg gatgcttccg
     1081 ccggacaagc gccgcccggg tttggcgctc aattcgttca gatttcgcct gaaggccgtc
     1141 agctcgtgta ccggtacgtc cgtaaccgcg agcctctctt ccacgacgac ctgtaaagca
     1201 gcgtgaccgc gggtttgggc tcgagcaggc gggcgttgcg ctcggaggga gggcgtgtac
     1261 tcagcaacgt gaagcgttgg tggacgcgga gcgctctgcc ggcccgtgcc tggttggttc
     1321 tgttcgtgct gctggcgtcg atcggggttc cgcgccccgc gcgcgcgggg gatccgtacc
     1381 tgcgatttcg gacaatccat actccgcact ttcgcgtgca ctaccacggc ggtctcgagc
     1441 gacttgcgag gcgcgccgcg cagcaagccg aggccattca cgaggcgctc gttcctgatc
     1501 tcggttgggc tccatcggag cccactcaac tcgtactcgc cgacgactcg gatttcgcga
     1561 atgggtatgg ctcgggggtg ccttataatg caatccgatt gcatgtgtcg gcgccggacg
     1621 acatgtcctc gctgcaagat tacgatgatt ggctcgccaa tatgatcacg cacgaataca
     1681 cccatattct gcacatcgac aatttgtccg gtatccccgc gctcttgaac acgctgttcg
     1741 gccgcgtcag cgtgccgaat caaagccaac cgcgctggat tttggaaggc ttcgcggtgg
     1801 ccatggaatc tgctcactcg agctcgggcc gtctgcggag cactcttttc gacatgtatc
     1861 tgcgcgcaga cgccctggag gataacctgg cggcattgga cgaaatcagc ggtcccacgc
     1921 ggcgctggcc cggcggtgag ctctggtact tgtacggcgc caagttcgtc gagtggatca
     1981 cggagactta cggaccagac actctggcgc gcatcgcttc cgagtacgga agtatgccga
     2041 tccccttcgc gatcaatcgc gcggtgcgcc gggtgacctc gcgaacctac cctgagctgt
     2101 acgccggatt tcaaagctcg atcgccgagc gcactcgaga gttcgtggcg ggcgttcgtg
     2161 cccgcggttt gcgtgagggg cgtcgtttga cctttgggca gcggatcagt cgcgcccccc
     2221 gcttcgtacc ggccgcgtgt cgcgagaccg cgtccccgca agtcagctat taccgcgacg
     2281 attcgctcgg caaacctggc ctgtatcgct tcctgctcgg cagccacgga gttgcgtcac
     2341 cggagctgat tgcccgctcc agcggaggcg cgcacagcta tacgccagag tgccagatcg
     2401 tcttcgattc tctggcgccg agcgcacggt actatcagtt cctcgacttg ttccgggtgc
     2461 ccccccgagc gcacgacatg tcacgcttcg atttgcggcg cgagaggctc accgtaggcg
     2521 cgcgagcgcg cgagcccgac gtcagccccg acggacggca gctcacgtac gtcaccaacg
     2581 acgcgggcac cacgacgttg aagctcgccg aactcaccgc tcgggggata gaacgtgaac
     2641 gagtgctcgt tccgagcgcc gagttcgagc aggcctatac gcctcggttc tctccagatg
     2701 gtcgccgcat cgcgtacagc gcctggacgg cgggtggctt ccgcgacatc cgcgtggtgg
     2761 acgtcgcgag cggccgcttt ttcgagctct ggcacgaccg cgcgatcgac cagcaaccca
     2821 cttggtccag agacggctcg accctctact tcgtctccga tcgcaccggc gcggccaacg
     2881 tttacgcgta cgagcttgcg agcggccgat tgtggcaagt cacgaacgtg ctcaccggcg
     2941 cctatatgcc cgagatctcc gacgatggac gcactctatt gtacgtcggt tacacgtcgg
     3001 gcggcttcga tgtgttcgcc ctggagctcg atccctcgaa gttcctggaa ccgatcccga
     3061 gcgtcgcgcg agcggcacac ccaatcgctc atcgagcgcg cgcgctcgac gcacgcgggc
     3121 aacgtccctt tgcggatcag ccttacgacc cgctgcccac cttgaggcct agagcctatc
     3181 gcctcgagta cggtccgggc acctttggcg acgcgatcac ggtcaagacg cgcggctccg
     3241 acgttctcga ttatcatacg atcctcgcca gcgtgatcct cgaggcacgt gagcccgact
     3301 ggcgcggctc actcgactac acgtacgatc ggctgccttt cagcctgcat ctcagtgcct
     3361 tccacgacgc tgccccgagc tacgtatacc ggttcgagga gccgcgcgag cggcacgtcg
     3421 actacagcaa cggggtgacc tccggcatcg gctggagcat ccccggcgaa tacgataccc
     3481 aaacgctggg atttagcttc actacggccg gtatcacccg agcggaggcg gttcccaagc
     3541 cacgcgaacc ctacgatccg ctgccgcgca ctacgagcgt gggtctctat gcaggcgcgc
     3601 gcctcgcgta tagttacggc aacgcggagg gcagtcttca cggggtgagc gccgagcgcg
     3661 gcttcaatct attcctcgca ctggacgttg ctgatccggc tttcggtagc gattggcgac
     3721 tcgccgcctt cacgggtgcg ttgaccacct atttctcgat gccatgggct cgtcatcacg
     3781 tactcgcgtt ggcgctgtcg ggcggcggct cgagcgggag ttatcctaga tcgtactttc
     3841 atacgggcgg gttcgtcgat cacaacgcct tcgatgtatt ccgtaccggc tttcggcaaa
     3901 gcggtttcgt gttgcggggt tacgagccgg ggcagttcat cggcagtgag tacactctgc
     3961 tcaacgtgga gtaccgcttt cccctgctgt atgcggaccg aggcctctcc actttgccgc
     4021 tcttcctgca gcaggtgagc ggtacgctct tcgcggacta cggcggcgcc tacgagcaag
     4081 tcgatcccaa agacccgttg cgcgtcatgc atctcggctt gggcggcgaa ttgtggctag
     4141 aactcgtgct cggctatcgc tactccggct cgctgcgcct aggagttgcg cggggaacag
     4201 gagagatggc gcacggcgtc cagtcctact tcgtcgccgc cgccgccttt tgacgagagc
     4261 gcggccgcga gagcccacgt cctcgttttt ctttcccgcg cgcgcgcact cgaggtaacg
     4321 agcgatatgc gcgcgatccg gtctttcgcg acggtatcct tgagcaccgc tttgaccggc
     4381 ggtgttctgc tcggtggctg cgtgttgcca ccgtctgctt cggagcgagc cgcggacgcg
     4441 gcgcgagagg tcaacgttgc gagccgtttt ggtcgaatgg atcgggcaat ggaactgacc
     4501 tcgaccggct tacgcagctc gttcctgcag catcgggccg catggggtaa agacattcgc
     4561 atcttcgatg tcgagctggt cgcattgcag cttccgtcga aggacagggc tctcgccgaa
     4621 gtcgagtatt cttggacgcg cactcgggag acggagctac gctcgacgcg agtggctcag
     4681 gagttccacg atcctggcgg tggcttccga ctcgttcgtg agcgccgcat agccggcgac
     4741 aagggcctgt ttgcggaacc gggagcaggc ccgagttcta gcgcgaaggc atccggagac
     4801 gtgcaatttg cgaccaaggt cattccgcct tcggagtgac tctgccctgg ggagccgacg
     4861 ccgtgaacca gcgcgaatgg cgatagcgcc ttcaaccaaa ggcgtcgcgc tcggcgacga
     4921 gcgccgagcg gatcccagaa acacgaccga agacgacgtt cagcgcttct tggacggttt
     4981 cttcttcgag cgctccgcgc gaacgctgtc ggcgcggcga gccaaacgag ccttcttgga
     5041 acgctgggct ttgcggcgga ggctcttttg cgagtggcga cgatcgaact ttcccatcga
     5101 gaagctttcc tgttggcaac gcgagcgcga ggcgcacccg cgacagcgga ttagcgcgct
     5161 ttgggtcgca acgaaagcgg gaactgctcg actcggccta tcgttggccc ccgcagaggc
     5221 acaaatcgag ctcggtttgg ctcattgcct gctttttggc gagcagcacg cgacggcaga
     5281 gccttcacga aagacccggc gccactccgc acggcagtgg gttcaggtag gcacccattc
     5341 ctgcgtcacg acgaccgcgc agctcaagcc aagcgccgtc tgagcaacgc gcaagctcgc
     5401 gaatttgcaa gagcggcaga ggaagctacc gctcggacgc agggttagcc gctcgagacg
     5461 tcttcgagag cgcgttcacg atcccgcacc tcgagacggt aacgactgtt gacggtctcg
     5521 atgtagaaaa cgtcacgtgc cgccaaccga agaacccgct tcacgggagt cgtgacgtac
     5581 tcgtgcattc catctgagaa ctcaatcacg actacagagc cttttcgcgg tgcacgcacc
     5641 aaccgcccgg cgactgcccg cgatcctcgc ccaagctttc taaggacgac ttgcacaatg
     5701 agtactctag caatccccat gcgcgcgtcc accgcttcgt cacgcttgcg atcatcaccc
     5761 tgcgagtgtc tctgtctgag tgggatttga cgtgagcggc ggggggtcaa cgtgagacag
     5821 gcaaatatcg ggcgaattcc gccgccgcca tacgttttgc tcggaagcgc ctaaggccgt
     5881 gctcggggcc ccggcgatac tgagtgcgta gtgaaaacac atggagtttc tcggatagag
     5941 ctccactttt gtgcaggcct ttcgagcttg cggagcaccc gaattccgtg gcgatttggc
     6001 tgctcgcgtc ggattcccac ttgagccgca ctggatgcga accattgttt cgaggccttg
     6061 cgttgacgag ctccgccctg gcgccgtcga gttccgcgct ttttcgccag cgggaaagct
     6121 caccaaatct ctggaattag ggcgaggaat gccactttgg ccgcgggctg cgcacatcac
     6181 gaaggtgatg cgatttctca tggtacggag ttctatcggc gccgtcctgt ctgtctggac
     6241 cctggccgcc gccgccgcgt gctttggggc gaacacggat ccgctccaat tcgggaaatt
     6301 gggtggaccg aagcggttcc cctgagacac gccttactcg ggggtgaaac gccgctcagg
     6361 atggcggcgt cacccctttc gcgccgtgac gtgcgaagta cgcgcgcagc acgtcacgag
     6421 ccagctgggg ggctttgatc tgccaagtcg gcgtattcac tacgagggta ctgatagcga
     6481 cctcgggttt gtccgccggc gcaaagccaa tgaaccaagt gtagtgtcgg ttggcttcgt
     6541 ggtcgctcag ggtgcccgtc tttcccgcca cgtcgatgcc gggcaagtaa gcactccccc
     6601 gctcgtcgtg aaaggccttg aaggcggaac cgttggagac ggtctgcgtc atcatctgcg
     6661 tgagctcggc ggccgtggag ggcttcagcg cgcgccgcag cacgcgcggc tccagctcgt
     6721 cacgccacac gatgtcgcgg cccttcagga tgctcgagac cacccgcggc tggagcgcaa
     6781 ctccgccgtt ggcgatggtt tgggccaaca ccgcgccggc gagcggggag agggtggtgt
     6841 tccagaagcc ggcggaggtg cgcgcgaact cgactggttc ggcggggatt tgaagcttcg
     6901 gcgcttgatt tggcaccacg aacggcacgg gcgctccgaa accgagtgca ccccccatcg
     6961 cggtgagatc ttcgggcgtg aggtgctttt gagctaatcg cccgaacacc acattcagac
     7021 ttcggcccat ggcgatgccg atcgtcgcgc accatcgatc acgctgcggg ttgtcctgaa
     7081 gctcgtcctg cgagatacgg ctcttcccgc cgtggtagca ctgctccgtt tcggcgttga
     7141 gtccggcgcg ctcgacgagg gccgcccccg taaccacctt gaatacgctg gccgcgggtg
     7201 cttccgcgcg ggcgttgacg tcgaatggct ctccctccgc cacatagctt gcgtaggcca
     7261 gcaccttgcc ggtctttacg
//
LOCUS       DASTKU010000173        12886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1817234, whole genome shotgun sequence.
ACCESSION   DASTKU010000173 DASTKU010000000
VERSION     DASTKU010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12886
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817234"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="VFQ61_06210"
     CDS             <1..1345
                     /locus_tag="VFQ61_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9954075.1"
                     /translation="IRVGGPYYAQIVQSKDLIADILPPPAYIIESYLLVFQAANSTDP
                     NEQKVLTQRLARTETEYWQRQQVWREQLGESRMKTTLVVGSAQPAKLFFQLIHERFLP
                     ALERGEVQTARDLANRELRDAYNEHRRAIDEVVQAATRYAADREREANSATESSSRLE
                     TAIGICGLLLGIAFGVLMIRALNRALSQVARDLNGRANRSADSATQVSSSSEAVARGA
                     SRQAASLQETSASLEQMSAMTKRNAENASTAKDFSSRTRSAVVTGVAEIGEMRQAMEA
                     IRNSSDQVGKIIKTIDEIAFQTNILALNAAVEAARAGEAGAGFAIVAEEVRNLAQRSA
                     VAARETSDNIAAAVENSERGVRVSAKVGLSLEYILQHMSTVDTLIGQIAQASVEQSQG
                     IGQLNVAMSDIDAVTQSNAGSAQEAASAASELTAHADAVKRNVSDLLGLIHGRAA"
     gene            complement(1764..2540)
                     /locus_tag="VFQ61_06215"
     CDS             complement(1764..2540)
                     /locus_tag="VFQ61_06215"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HET9954076.1"
                     /translation="MRIHGKTVLSGVVSVVACVAITTVAVLFLIRTELTRQAHVYQDQ
                     KIKMLSEMLRQKGEPRVVDGKLMFGDYVVNNNYEVVDKLAAIAGGTATIFLGDTRVST
                     NVMKDDGTRAVGTPLIGVAKEAVIDRVQSYRGEANILGVPYFTAYDPLRDANGKPFGV
                     LYVGVKQDEFFQSFRRLVAVATGVAVVMALGFGVLIWYMAGRLLGRLQRLTKAADAVS
                     IGEELETSLATPEDDEVGDLSRALDRLRESVASAIKRLDA"
     gene            complement(2639..3406)
                     /locus_tag="VFQ61_06220"
     CDS             complement(2639..3406)
                     /locus_tag="VFQ61_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954077.1"
                     /translation="MENILQDLHDLEGVNGAILADANGQVVAFRTHSMYDAALRQQVS
                     RAVRSAVDSVKLTHEDWDSINVQFTEGRLMIRNLANAPASPSFTLTLIADGRLNPSFA
                     NVAIRVAIGKLKTALQAGPPAPSPMQVAPSPPAAPLAGSAAAMPLSASQPNVPVSEVA
                     NSGLSWSGLGSSTASASGVEVADAAASAALTTCTKALAKCVGPMAKIFVKEAVRKLCP
                     GRPFSKDMAPSLVAELAKQISNPATAAQFQTALLKSL"
     gene            complement(3415..3732)
                     /locus_tag="VFQ61_06225"
     CDS             complement(3415..3732)
                     /locus_tag="VFQ61_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET9954078.1"
                     /translation="MLDLNTIAALPEVIGVALSDSSGALLETTGEIDGEACGAVHSFS
                     VMAFTQAGEALGLGEFQRASVTGPQKACLIAVQEGAVLGIYVDPGKQFSVVEKKLQNI
                     LQK"
     gene            complement(3955..5838)
                     /locus_tag="VFQ61_06230"
     CDS             complement(3955..5838)
                     /locus_tag="VFQ61_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9954079.1"
                     /translation="MDAESLIGQKLGNFRLERVLGLGRMGVVYLARDEALLRPTAVKI
                     LSWRLPEQHGLDPEAWFLAEARNAARINHPNVVQIYGVAKHIQQCYIAMEYVTGMAAD
                     AWLKKDVRFSVQRATEILIQSASALQAAHDAGVVHRDIKPGNLLITSDGVAKLGDFGM
                     ALSVAAEPRAGQVRAGTPFYTAPEIWRGQAASVASDIYALGATYFQLVTGHLPLNANT
                     LEELIAAHLNAPLPDPQQYVPDLPGECVRIIQHCLQKSPTERFTSAQALSWEARGLLR
                     ELTSAVRQSAVSPPNLERRSDSPPRRDSKAPATPRSVSPPRPIGSEPFGSISQELRAL
                     IADASVPVVLLSGERGSGKTTLARGIAEEYERWGAVAWASANDENKPIAQSLLAGLGG
                     VPAATVSNPSSGIELLLNRLEQGRSGVGPSALLIIDDVAPSGARLSEVAALVKASVTT
                     RAFRLLVVSPPEFRERAGLIGANLRLSSVHVPLLTTQQTLRYLSEWLQAMSAKERVMI
                     TPDAALIIAYRSKGNVRRMNQMAASMLEGATLAGRALISSWDAWSAPENPVVSVGAPQ
                     QKPKGWPTADVLQILNLQRAAAGLPVRNPSVEIRTVSAGSVSPGVSSSAVSPGSAPPR
                     ERS"
     gene            complement(5856..8246)
                     /locus_tag="VFQ61_06235"
     CDS             complement(5856..8246)
                     /locus_tag="VFQ61_06235"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9954080.1"
                     /translation="MVEPSKLTSAEAPSESQISATDSTAGPRVLDAGAVLTHVPGARG
                     CCVGSRQGHISSSEGEAEGARSVLASSAASFGDLGQLGELLGIGGSALVTFSSPGCTV
                     VMMFRAEYVIAVELEPSRPLKNIEFALKNTDWDGLSEWALTDRDIEYVAGHQAERPRV
                     TPSRIPPPIPAGSAKGHRSSAPPLDRAGRRAIGEEWAELRRALSKGQLSTARRLGARI
                     QSALSPREGTPGSVDAPQLLQTLVEAIAGILAGDHAAGLKKLEALAQTSQTGPSLKWA
                     AQVWSARASQCYAAGLDAARTYATSALKLAERLDPEAVAVSHLVLAEVASHAGDWARV
                     LEFASRARQMFMGPPAARELRVAKAQQELASCWLLEARAHAACSRRTDSLKAAQSAHD
                     CLPSWAPPVTFMLRLALSAGQTPAGQPFSAELQQAEKALDALLAVSPAPADAERDRRI
                     LSGVRTGVLPAAVACQYLELIDGPANPATLQELEELASKFPRVAQFRASLGWMLLRAG
                     KREAAKDVFERLSRRGDLPDDMRASVLLALGCLATAENSEEHPSAKLRAAVEAAPKSS
                     GSVSPPASVPTSKSRQPVAFDYRNHPSVPPSSPAPRANGARQALMGPVFSGNLELFAL
                     PELLEFLRAGQRTGTLVCSSGAAIGAIHLRAGRLTGAAAPNTPKLGEQLVKSRVLSAE
                     QLARAKPGQTDVALASNLVQLRLVKPEQVRQALREQINSALLELLAWAEGQFAFNPEP
                     ANPGGPPSPTGPLAPTELANQTGSSELEVELDPQLLLLEIFKELDERANRPGSSAP"
     gene            complement(8707..9858)
                     /locus_tag="VFQ61_06240"
     CDS             complement(8707..9858)
                     /locus_tag="VFQ61_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9954081.1"
                     /translation="MSSSKLSKRCVRDGLVWLLALCLLVFCCQSPSEGSPGGETHFLT
                     RCDPKAPCGSGLACLCGVCTQACADQAACGSFPSAACVAASEAGCFDSNGGGYCDVRC
                     NLDADCRVVSPAHRCDSGVCRAGTVSVEPPSSASGGSGASGASGASSGSGGSGGSGGS
                     AAVGSGGVDHGGAPSLCSGEAVEANQVLWLGDSFFALTHQITGYLEELARGAGALAPG
                     ERYRDNSRQVDNTLALAGNGIAKQYDAGAADGPVKIVIMTGGGADVLLGSCDVLDSAC
                     PLLTQAVSGAQELLARMERDGVRDVVYVFYPDPLDAGLRAKVDALRPLIQSVCEASPV
                     PCHWLDLRPTFEGRYDQYLQADGITPNDAGAQASAAALWALMRENCIAQ"
     gene            complement(9848..10849)
                     /locus_tag="VFQ61_06245"
     CDS             complement(9848..10849)
                     /locus_tag="VFQ61_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954082.1"
                     /translation="MTIELLIHAIVRQTTILIAQLATSRGARAPLAQIANQVFVDLVR
                     ELDRQGVSRKVSADMFGLGLRTYRRKIQRLSESSTERGRSLWEVVLEHIRNQGLVTRL
                     EILTRFSRDDEAQVRAVLRDLCESQLVFSSGLGPSTSYRAASEDELAMLHRKHGSEGT
                     DELLLAIMYREGPLTVKEIAEKAQAEIVDVETAIARLSSAARVERSEQDGTVRYSASS
                     LIIPLGAPVGWEAAVFDHFKALVTTILIRLGEKRAPAPEDQVGGSTYTIDLWRGHPLA
                     EEVYGTLSKLRSELSDLRTRVAEFNEKQESPDLHSRVVIYVGQCVIQDGQEDKASVE"
     gene            complement(11036..11446)
                     /locus_tag="VFQ61_06250"
     CDS             complement(11036..11446)
                     /locus_tag="VFQ61_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HET9954083.1"
                     /translation="MITLRISHPRPEPLVAEAARLASALTERWLEKDPAVTAVEVTVT
                     PTSHWFVAGRTLAEQQLAAFFLEVRISAGTNTKDQKARFVAEAFQGFRALLGPLNSES
                     YVYVIDANPDAYGFGGLTQERRYIEGRPRAPGVG"
     gene            11526..12452
                     /locus_tag="VFQ61_06255"
     CDS             11526..12452
                     /locus_tag="VFQ61_06255"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET9954084.1"
                     /translation="MSEHLDPDLLRTFLQAVDAGGLMRAAPRVGRTRAAVSLQMARLQ
                     AAVGQPLFRKVGRQLQLTPAGEALAGWARQILALNERALAAIRHTQTLERIRVGAPQD
                     VVERWLPQVLAQFSKAHPHVRLELVVGSSRVLSDLEREGRLDLCVRFGLAEEANGERL
                     GQTPVRFFAARSFVLEPTEPLALVLLDAPCIFRTSALRVLDEAGMPWRIAATSPSVSA
                     LWAAVAAGLGVTARPAISAPRGVREFEPPGVPALPHATLSLSGSMDSTALRALRRGIM
                     SELRARLSRRGRTSAGHATGSGTSKSPTPKKA"
     gene            12505..>12886
                     /locus_tag="VFQ61_06260"
     CDS             12505..>12886
                     /locus_tag="VFQ61_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954085.1"
                     /translation="MNAAVQALLYRPASMMRLSNALAGAAATMSLACGGPQEPSRALA
                     PRERAPCLSLPERCAGESGVFPVSGYSPRLPAHELAPIAATLADADVVGLGEAAHGSA
                     GFIGIKVRLSRELIEHYGFRVIAWE"
BASE COUNT         2385 a         4298 c         3935 g         2268 t
ORIGIN      
        1 gatccgcgtg ggaggtccct actatgcgca gattgtgcag agcaaggatt tgatcgccga
       61 tatcctgcca cccccggcgt atatcatcga gagctaccta ttggtatttc aggccgcgaa
      121 tagcacggac cccaatgagc agaaggtcct gactcagcgc ctcgcccgaa ccgagaccga
      181 gtattggcag aggcaacagg tgtggcgcga gcaattgggt gagtcgcgca tgaagaccac
      241 gttggtggtt gggtctgcgc aacccgccaa gctcttcttt cagctgatcc acgagcgatt
      301 cctgcccgcg ctcgagcgcg gcgaggtcca gacggcgcgg gacttggcca atcgggagtt
      361 gcgtgatgcc tacaacgagc accggcgagc catcgatgag gtggtgcagg ccgcgacccg
      421 ctacgccgcg gatcgtgaac gcgaggcaaa ctccgcgacc gagagtagct cccggctgga
      481 aaccgcgatt ggcatctgcg gcctgctgct cggcatcgcg ttcggcgtgc tgatgattcg
      541 agcattgaac cgcgcgctct cccaagtggc gagagacctg aacgggcgcg ccaaccgaag
      601 cgcggattct gccacgcagg tatcctcctc cagcgaagcc gtggcgcgcg gtgccagccg
      661 gcaggcggct tcactgcagg agacgagcgc ctctctggaa cagatgtcgg cgatgaccaa
      721 acgcaacgcg gaaaacgcct ccactgcgaa ggacttctcg agtcgcaccc ggagcgccgt
      781 agtcacgggt gtcgctgaaa tcggcgagat gcgccaggcc atggaggcca tccgtaactc
      841 gtcggaccag gtcggcaaga tcatcaagac catcgacgag attgcgtttc aaaccaacat
      901 cttggctttg aacgcggcgg tcgaagcggc acgagccggt gaggccggcg ccgggttcgc
      961 gatcgtggcc gaggaggtgc gcaacctggc acagcgctcg gccgtggccg cacgcgagac
     1021 gtccgacaac attgcggccg ccgtcgagaa cagcgagcga ggcgtgcggg tctccgccaa
     1081 agtcgggctc tcgctcgaat acatcctgca gcacatgagc actgtagaca cgctgatcgg
     1141 acaaatagcg caggcatccg tggagcagag tcaagggata ggtcagctca acgtggcaat
     1201 gtccgatatc gatgcggtaa ctcagtccaa tgctggcagc gctcaggaag cagcttcggc
     1261 cgcgtcagag ctgaccgctc acgccgatgc cgtaaagcgc aacgtctccg acttactcgg
     1321 attaattcac ggccgcgccg cctgaacgct gctctctgat ccaatcgaag cgggccggcg
     1381 cagcacgcgg gacgcaatgc tgcgaagtag cgactccccg ctatccacaa cccacgactc
     1441 cgcaactcca cgaggcaccc gccacgccac gcggtcgcaa cgcatgagaa gcgcggccca
     1501 ttcgcgcgcc gccgccgaat ggaccgccca ccacgcgccc ggagagaggc tcgaacacgg
     1561 aggtgcgaac acggaggtgc gaacacggag gtgcgaacac gtagctccgg cgcgacgaat
     1621 tcaaggccac aaaacacgaa ggcccccgga taaccagggg cctcttggca cctcacgcta
     1681 actgagccgg gattctgcga cttccaatcc tcgctaaact ccagaccgag gtgccctgaa
     1741 ccaactcgtc gttgcgggcc ggatcaggcg tccaagcgct tgatcgccga agcgacgctc
     1801 tctcgtagac ggtcgagagc acgagacagg tcgcccacct cgtcatcttc aggggtggcg
     1861 agcgacgtct cgagttcctc tccgatactg acggcgtcag ccgccttggt gagccgctgg
     1921 aggcgaccga gcaagcggcc cgccatgtac caaatcagaa caccgaaccc gagcgccatc
     1981 accaccgcga cgccagttgc cacggccacc aagcggcgga aggattggaa gaactcatcc
     2041 tgcttcacac ctacgtacag cacgccgaag ggcttgccgt tcgcatcccg caacggatcg
     2101 taagccgtga agtagggcac gccgaggatg ttggcctcgc cgcgatacga ctgaactcga
     2161 tcgatcacgg cttccttggc cacaccgatc aacggagttc ccaccgcgcg cgtgccgtcg
     2221 tccttcatga cattggtcga gacgcgcgta tctccgagga agatcgtagc cgtgccgccg
     2281 gcaatggcgg cgagcttgtc gaccacctcg tagttgttgt tcacgacgta gtcgccgaac
     2341 atcagctttc cgtctacgac ccggggctcg cccttctgtc gcagcatttc gctgagcatc
     2401 ttgatctttt gatcttgata cacgtgcgct tggcgggtca gctcggtgcg aatcaaaaac
     2461 agaacggcaa ccgtcgtgat cgctacgcac gcgacgaccg aaaccacacc agaaagaact
     2521 gtcttgccat ggattctcat ggtcccctta aactcctagt agaaaggtat cgctcgtcga
     2581 agagcggtaa tcagtgacgg agagcacgtc cagtctccgt cactctatcg ggatctgctc
     2641 agaggctctt cagcagcgcc gtctgaaact gcgctgccgt agccggattg ctaatttgtt
     2701 tggccaactc ggccactaaa ctcggcgcca tgtctttgga gaacgggcgc cctgggcaca
     2761 gcttgcgaac cgcttccttg acgaagatct tcgccatggg gccaacgcat ttggccaggg
     2821 ctttggtgca agtcgtgagg gccgcggacg ccgccgcgtc tgccacctcg acccccgaag
     2881 cgctggccgt cgaactacca agccctgacc agctcaggcc gctattggcg acctccgaga
     2941 ccggcacgtt gggctgcgaa gcagacaagg gcatcgccgc cgcgctcccg gcgaggggcg
     3001 ccgcgggcgg gctcggcgcg acctgcatgg ggctcggcgc gggcggcccc gcctgcagcg
     3061 ccgtcttgag cttgccaatg gcgacgcgaa tcgccacatt cgcgaaggag ggattcagcc
     3121 ggccgtctgc aatcagcgtc agcgtgaacg aagggctcgc gggggcgttc gccagattgc
     3181 gaatcatcaa tctaccctcc gtgaattgca cgttgatgga gtcccaatcc tcgtgcgtga
     3241 gtttgaccga gtctacggcg ctcctcaccg cccgactcac ctgctgtcgc aacgcagcgt
     3301 cgtacatgct gtgggtgcgg aaggcgacga cctgcccgtt cgcatccgct aggatcgccc
     3361 cgttgacgcc ctcgagatcg tgcaagtctt gtaggatatt ttccatgtgc agcgctactt
     3421 ctggagaatg ttttggagct tcttctcgac cacagagaac tgtttccctg gatccacgta
     3481 aatgccgagc acggccccct cttgaacggc gatgaggcaa gccttctggg ggcccgttac
     3541 ggaggcgcgt tgaaactctc ccaaaccgag cgcttcgccg gcctgggtga aggccatgac
     3601 cgaaaacgag tgcacggctc cgcacgcctc gccgtcgatc tcaccggtcg tttccaagag
     3661 agcaccggaa gagtcactca gcgcgacccc gatgacctcg ggcagggctg cgatggtgtt
     3721 gaggtccagc atgacttcgg taccttctcc cttttttccc ttttcgtccc tccggacgcg
     3781 gttcgcccgg cgagtgaagc ctacccgtgg gttgacccag gggctgcggc gagcgcgtct
     3841 cccgtgctcg ccagcatttt cggctccgct tcatattcgt tacgggcggc tcacgtgatc
     3901 tttttagggc actccgtcac tatccgtcga aaccgggcgc tccagcatat cgaatcatga
     3961 ccgttcccgg ggcggcgctg aacctggaga gacggcggag gaagaaacgc cgggagacac
     4021 cgagcccgcg gagaccgtgc ggatttcgac tgacggattt cgtaccggga gaccagccgc
     4081 ggcgcgctgg agattcagga tttggagcac gtcggcagtg ggccaccctt tcggcttttg
     4141 ttgcggagcg ccgacactga cgaccgggtt ttcgggtgca ctccaagcat cccaggagga
     4201 aatcaaggct cgtcccgcta gcgtggctcc ctccaacatg gacgccgcca tttggttcat
     4261 ccgccgcacg ttgcctttgc tgcgatatgc aatgatcaac gcggcatcgg gcgtgatcat
     4321 tacccgttct ttggcggaca tggcctgtaa ccactcgctc aaatatcgta acgtctgttg
     4381 cgtggtgagg agtggcacat gcacgctgct caaccggaga ttggcgccaa tcaacccggc
     4441 gcgctcgcgg aactcgggcg gcgacaccac gagcaagcga aaggctcggg tcgtaacgct
     4501 cgctttgacg agcgccgcaa cttcactcag cctcgcccca gagggcgcca cgtcgtcgat
     4561 gatcagtagc gccgacggcc ccacgccgga gcggccctgc tccaagcgat tgagtaacaa
     4621 ctcgatgcca ctactcggat tgctgacggt ggccgccgga acgcccccca atcctgccaa
     4681 gagcgattgt gcgatcggct tgttctcgtc gttggcggag gcccaagcca cagctcccca
     4741 gcgctcgtac tcctcggcga tgccgcgagc cagcgtcgtc ttcccgctgc cacgctcacc
     4801 ggagagcaac accacgggta cgctcgcatc ggcgatcaac gcgcggagct cttggctgat
     4861 cgatccaaac ggctcggagc cgatcgggcg cggcggggag acggatcggg gcgtggccgg
     4921 agctttggag tcacgtcgcg gtggagagtc gctgcgtcgc tcgaggttgg gaggagatac
     4981 ggcggattgc cgaactgcgg acgtcaactc gcgcagcaaa ccccgagcct cccagctcag
     5041 ggcctgcgcc gacgtgaacc gctcggttgg cgacttctgc aggcaatgtt gaatgatgcg
     5101 cacgcattct cccggcagat ccgggacgta ctgctgcgga tcgggaagcg gagcgttgag
     5161 atgggctgcg atcagctcct cgagcgtgtt ggcgttcagc ggcaggtgcc ccgtgaccaa
     5221 ctggaagtag gtggcgccaa gcgcataaat atcgctcgcc acgctggccg cttgtccacg
     5281 ccagatttct ggcgcggtat agaacggcgt gcccgcgcgc acctgcccgg cacgcggttc
     5341 ggccgccacc gagagcgcca tcccaaagtc cccgagcttg gccaccccat cggacgtaat
     5401 cagcagattg ccgggcttga tgtctcggtg caccacgccc gcgtcgtggg ccgcttgcag
     5461 ggcactcgcc gattgaatca ggatctcggt cgcgcgctgg accgagaagc gcacgtcctt
     5521 cttgagccag gcgtcggcgg ccatgcccgt gacgtactcc atcgcgatgt aacactgctg
     5581 aatgtgcttt gcgacgccat agatctgcac cacgttaggg tggttgatgc gcgcggcgtt
     5641 gcgcgcctcg gccaagaacc aggcctccgg gtccaagccg tgctgctcgg gcaggcgcca
     5701 agacaggatc ttcactgcgg tcggacgcag tagcgcttcg tcgcgcgcca ggtacaccac
     5761 ccccatccgc cccagaccga gtacacgctc gagccgaaaa ttcccgagct tttgaccgat
     5821 cagactctcg gcatccacgc ttccctcacg ctggctcagg gcgcgctcga acccgggcga
     5881 ttcgcccgtt cgtcgagctc cttgaagatc tcgagtaaga gtagttgagg atccagctcg
     5941 acctcgagct cggatgagcc ggtttggttc gccagctccg tgggcgcgag cggtccagtt
     6001 ggactcggcg gcccccccgg atttgccggt tcggggttga aggcaaactg cccctctgcc
     6061 caggccagaa gctcgagcaa ggcggagttg atctgttccc ggagcgcttg cctcacttgc
     6121 tctggtttta cgagccgcag ctgcacgaga ttcgaggcca gcgcaacgtc cgtttggccg
     6181 ggcttcgcgc gggcgagctg ctccgcactc aaaacccggc tcttgactag ttgctcgccg
     6241 agcttcggag tgttcggcgc cgccgccccc gtcagcctac cggctcgcaa atggatggcg
     6301 ccgatggcgg ctccagagct gcacacgagt gttcccgtac gctgccccgc gcgcaggaac
     6361 tcgagtagct ccggtagcgc gaagagctcc aggttccccg agaagaccgg ccccatcaac
     6421 gcttgccgcg caccgttcgc gcgtggggca ggcgaggagg gcggaacgct cggatgattg
     6481 cgataatcga acgccaccgg ttgtcgagac ttggaggtgg gaaccgaggc cggggggctt
     6541 acgctgcctg aggattttgg cgccgcctcg accgcggccc ttagtttggc actcggatgt
     6601 tcttcggagt tctccgccgt ggccaagcaa cccagcgcga gcaggacgga ggctcgcatg
     6661 tcatcaggca agtccccgcg ccgactcagg cgctcgaata catccttggc ggcctcacgc
     6721 ttccccgccc gcagcaacat ccacccgagt gaggcacgga actgcgcgac gcgcgggaac
     6781 ttcgaggcca actcctccag ctcctgtaag gtggcaggat tcgcggggcc atcgatcagc
     6841 tcgaggtatt ggcaagcgac ggcggccgga agaacgcctg tacgcacgcc cgacaagatc
     6901 cgccgatcgc gctcggcgtc agcgggtgcg ggtgagaccg ctagtagtgc gtccagcgcc
     6961 ttctctgctt gctgaagctc cgcagagaag ggctgcccag caggggtttg gccggcggac
     7021 agcgccaacc tgagcatgaa agtgaccggc ggagcccagg aagggaggca atcatgcgcg
     7081 gactgcgcgg cctttaggct gtccgttcga cgcgaacacg ccgcgtgtgc gcgcgcctcg
     7141 agtagccaac acgaggccaa ctcttgctgc gctttggcca cccgaagctc acgcgcggcg
     7201 ggtgggccca tgaacatctg cctggcgcgg gaggcgaact ccagtacccg cgcccaatcg
     7261 cccgcgtgac tagcgacctc ggccaacacg agatgactca ccgcgaccgc ctctggatcc
     7321 aagcgctccg cgagcttgag cgccgaggtc gcgtaggtcc gggccgcgtc gagccccgcc
     7381 gcataacact ggctcgcgcg cgcgctccag acttgggccg cccacttcaa ggacggtccc
     7441 gtctgagagg tctgggccag cgcttcgagc ttcttcaaac ccgccgcgtg atcgccggcc
     7501 aggatgcctg cgatcgcttc caccagggtc tgcaagagct gaggtgcgtc cacgctaccg
     7561 ggggttcctt cacgcggaga gagcgcgctc tggatccgcg cccctagacg ccgtgcggtc
     7621 gagagctgtc ccttcgatag cgcacgacgg agctccgccc actcctcacc gattgcccgg
     7681 cgccccgcgc gatccagcgg tggcgccgat gatcgatgtc ccttcgccga gcccgccgga
     7741 atgggcggag gaatcctgga cggcgtcacc cgcggccgtt ctgcctgatg gcccgccacg
     7801 tactcgatgt ctcgatccgt gagcgcccac tccgacagtc cgtcccaatc cgtattcttc
     7861 agcgcgaact cgatgttctt gagcggccgg ctcggctcga gctccaccgc gatcacgtac
     7921 tccgctcgga acatcatcac gacggtacag cccgggctgc tgaaggtcac cagcgccgac
     7981 cctccgatac ccaataattc accgagctgc cccaagtccc cgaacgaggc ggcactcgag
     8041 gccaaaacgc tgcgcgcgcc ctcggcctct ccctcggaac tcgagatatg accctggcgg
     8101 ctaccgacac agcaaccgcg cgcaccggga acgtgagtaa gcaccgcgcc cgcatcgagc
     8161 acacgcggac ccgcggtcga gtccgtcgcg gagatctggg actccgatgg cgcctcggcg
     8221 ctggttagct tgctgggctc gaccatggca gcgactcctt tgaagtccga ccgtcaccaa
     8281 gcatcagctc cctcgattgc atccgacgcc ttctcgcgtt catacgcgct gcctctgctt
     8341 ggaaaggcga accgggccca ctcgtggctc cacggtcagc acaccgagca catctcgcaa
     8401 tgatgcctat acgaaagctc gacaacgaga cgcgtacacc atcgtccacg tcatacggtg
     8461 acgagcatac cgcattaagt gcggccccgg tgcgctctgc ctcgatccgg atcgggttgc
     8521 tgatttgccc taggaagcgc cccactctca cgcgcgatgg ttgataaccg tcaccacttc
     8581 gcgcaccgcg acttaagatg tcaaagccag cacaatgagc tgtcggaaac cgccgtagcc
     8641 aacgggagag tgaaaggctc tcccgtattt gcgtctcgaa ggaagctcag aacagctatc
     8701 agagcttcac tgcgcgatgc aattttcacg catcagcgcc cagagggctg ctgcgctggc
     8761 ctgggcaccc gcgtcattcg gcgtaatgcc gtccgcctgt aagtactggt cgtatcgccc
     8821 ctcgaaggtt ggacgcaagt ccagccaatg acacggcact gggctcgctt cgcaaacact
     8881 ttggatgagc ggtcgcaggg cgtcgacttt ggcgcgtaac ccggcatcca gaggatccgg
     8941 atagaacacg tatacgacgt ctcgaacgcc gtcgcgctcc attcgagcca gcagctcctg
     9001 agcgccgctg accgcttgcg tgagtagtgg acaagctgag tcgagcacat cgcatgaacc
     9061 caggagcacg tccgcaccac cgcccgtcat gatcacgatc ttcacgggcc cgtcggcggc
     9121 tcccgcgtcg tactgcttgg cgatgccgtt gccggccagc gccaatgtat tgtcgacttg
     9181 gcgagagtta tcccggtaac gttccccggg agcgagagcg cccgcgccgc gcgcgagctc
     9241 ttcgaggtag cccgtgattt ggtgggtcaa ggcgaagaag ctatctccca accacaagac
     9301 ctgattggct tcgaccgcct ccccgctaca caggctcggg gcgcctccat ggtcgacccc
     9361 gccggaccca accgccgcgg agccaccgct cccgccactt ccaccgctcc cgctactcgc
     9421 accactggca ccactggcgc cactgccacc actcgcggag ctcggcggct cgacgctcac
     9481 cgtgcccgcg cggcagacgc ccgaatcgca gcgatgcgcg ggagagacga cacgacaatc
     9541 cgcgtccaag ttgcagcgca cgtcgcagta gcctccgccg ttcgaatcga agcaaccggc
     9601 ttcactggcg gccacgcaag cagcagaggg gaaacttccg cacgccgctt gatccgcaca
     9661 ggcctgcgta cacacaccac acaagcacgc caagccactc ccgcaaggag ccttggggtc
     9721 gcaacgcgtg aggaagtgcg tctcaccccc cggggagccc tcactcgggc tttgacagca
     9781 aaacaccaac aaacagagcg cgagcagcca caccaggccg tccctgacac atcgcttcga
     9841 taacttgcta ctcgacactc gctttgtcct cttggccatc ttggatgaca cactggccca
     9901 cgtaaatcac tacgcgcgaa tgcaggtcgg gcgactcttg cttctcgttg aattcagcga
     9961 cccgggtacg cagatcggac agctctgagc gcagcttgct cagcgtgcca taaacttctt
    10021 cggcgagcgg atggccgcgc cagaggtcga tcgtgtacgt gctaccgccc acttggtcct
    10081 cgggagccgg ggcccgcttc tccccgagac gaatcagaat cgtggtaacc agggccttga
    10141 agtggtcgaa cacggcggct tcccagccca ccggagctcc gagcgggatg atcagtgagc
    10201 tcgcgctgta gcgtacggtc ccgtcctgct ccgatctttc cacccgcgcc gccgacgaaa
    10261 ggcgcgcaat ggcggtctct acgtccacga tctcggcctg ggccttctcc gcgatctctt
    10321 tgaccgtgag cggcccctct cgatacatga tcgcgagtag gagctcgtcc gtaccctcgc
    10381 tcccgtgctt acgatgcagc atcgcgagct cgtcctccga agcagcgcga tacgacgtgc
    10441 tcgggcccag tcccgacgag aacaccaact ggctttcgca caagtcccgc agcaccgctc
    10501 tgacctgcgc ctcgtcgtct cgggagaagc gcgtcaagat ttcgagccgc gtgacgagcc
    10561 cctgattcct gatgtgctcg agcaccacct cccagagtga gcgcccgcgc tccgtcgagc
    10621 tctcgctcag gcgttgtatc ttccggcggt aggtgcgtag gccgaggccg aacatgtcgg
    10681 cgctcacctt gcggctcact ccctgccgat cgagctcgcg cacgagatcc acgaagacct
    10741 gattggcaat ctgagcgagc ggagcccgcg cccctcgcga ggtcgcgagt tgcgcgatca
    10801 agatcgtagt ttgccgaaca atcgcgtgaa tcagtagttc gatggtcatt gcgcctggtt
    10861 cggcctgggc attgtacacg accggcgccc cttcagaggg aggacgagga tgtcccccct
    10921 caccccacat ctttcttcga actcgcgtcc tgaaaagttc cccgttcggg actcctcacg
    10981 gcccccgcgc gctccaccaa tccgattctc cgggcggcgc gcccgaaaat gaaccctaac
    11041 ccacgcctgg cgctcttggc cgcccttcga tatagcgccg ctcctgggtc aggccaccaa
    11101 aaccgtaagc gtcggggttg gcatcgatca cgtacacgta gctctccgag ttcagcggcc
    11161 ccagcaatgc ccggaagccc tggaaagctt ccgcgacgaa gcgcgccttt tggtccttcg
    11221 tgttggtccc cgcactgata cgaacttcca aaaagaaggc tgccaattgc tgctccgcta
    11281 gcgttcgccc ggcaacgaac caatgactgg tcggtgtaac cgtcacctcg accgccgtga
    11341 ccgccggatc cttctccagc caacgctcag tcagggcact ggcgagccgc gcggcctcgg
    11401 cgaccagcgg ttcgggacgg gggtgagaaa ttctcaaagt gatcatcggc atcgacagtt
    11461 cgtagatcgc gcaaggttat cttcaacttg atagtattta acgattagtt aaggttctct
    11521 aaaccatgtc ggagcatttg gatccggacc tgctgcgcac ttttttgcaa gcggtggacg
    11581 cgggcggttt gatgcgcgcg gctccgcgcg tggggcgcac gcgggcagcc gtgagcttgc
    11641 aaatggcgcg tctgcaggcc gccgtcgggc agccgctatt tcgcaaggtg gggcgacagc
    11701 tgcagctgac accggccggc gaagcgctag ccgggtgggc gcgccagatc ctggccttga
    11761 acgaacgcgc gttggccgcg attcggcaca cgcaaacact ggaaagaatc cgcgtaggcg
    11821 ctccgcaaga cgtcgtggag cgctggttac ctcaggtgct ggcccagttc tccaaagcgc
    11881 atccgcacgt tcggctggaa ctggtggtag gcagcagccg cgttctttcc gatctcgaac
    11941 gggaggggcg gctcgatctc tgtgtacgct tcggattggc agaggaagcg aacggcgagc
    12001 ggctaggcca aaccccagtg cgcttcttcg ctgcgcgcag tttcgtgctc gaacccacgg
    12061 agcctctggc gctcgttttg ctggacgcgc cttgcatctt ccgcacgagc gcactgcgcg
    12121 tcttggacga agcagggatg ccctggcgca tcgctgccac ttcgccatcc gtgtccgcgt
    12181 tatgggcggc cgtggcggcc ggcctcggag tcacggctcg gcccgccatc tccgcacccc
    12241 ggggtgtccg ggagttcgag ccgccaggcg tgcctgcgct tccacatgcc acgttgtccc
    12301 tctccggctc gatggacagt acggcacttc gcgctctgcg gcgcggcatc atgagtgagc
    12361 tacgggcccg gctgagtcga cgcggtcgaa ctagtgcagg ccatgccacc ggaagcggga
    12421 cgagcaaatc tccgacaccc aagaaggcct gatctggacg gcctaaaccg aaatcgcgac
    12481 gccgaaaact cggctaagta gtgaatgaac gctgcggtac aggccttgct ttatcggcct
    12541 gcatcgatga tgcgcctctc gaacgccctc gccggagcgg ccgctacgat gagcctggcc
    12601 tgcggcggcc cccaagagcc gtcgcgggcg ctcgcgcccc gcgaacgggc gccttgccta
    12661 tccttgccag agcgctgcgc gggtgagtct ggtgtgttcc cggtatcggg ctactcgccg
    12721 cgcctgcccg cacatgagct cgctcccatc gcggccacat tggcggacgc ggacgtggtg
    12781 ggcttgggtg aggccgcaca cggctctgcc ggcttcatcg gcatcaaagt gcgactctcc
    12841 cgagagctca tcgagcacta cggttttcga gtcattgcct gggaga
//
LOCUS       DASTKU010000174        68267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_701301, whole genome shotgun sequence.
ACCESSION   DASTKU010000174 DASTKU010000000
VERSION     DASTKU010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 68267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68267
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701301"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFQ61_06265"
     CDS             <1..289
                     /locus_tag="VFQ61_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954086.1"
                     /translation="FQLEVQANGDGTARILKTRVNWSIGGLSLVINDDRGDQEFLRKV
                     QGKNEFVPITITFASQNTYQGRGQVVDELSFSSESSTADVTLTGEGELTKQ"
     gene            286..720
                     /locus_tag="VFQ61_06270"
     CDS             286..720
                     /locus_tag="VFQ61_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954087.1"
                     /translation="MNNVVALQTGTSATSLEVAEAEFQRFVAAMALDVDTSTMDEEDR
                     EEFKDKKRKIVRALMLGSLVIDEKGQPVYTPQVGDDRSPITFLEPRGSSLMAMDQRKQ
                     GHNVAKSFAVLADITKVSPQRFANMFNRDIQVCLALQALFLG"
     gene            1113..3104
                     /locus_tag="VFQ61_06275"
     CDS             1113..3104
                     /locus_tag="VFQ61_06275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HET9954088.1"
                     /translation="MAIGAGAAVAGLGIKSVVDTGAEFEQAIADVGAVSLMTAAQVGD
                     LRSKSLELGAATRYSATQVARGFESMGRAGFNNEQMLSGISGILSAAAAEGAEIEETA
                     DTVSAVLKGMRLDQGDLGAEANRVANVLALASARTKASLLSLGESMKTLGPTAAQFNI
                     PLETAVGMVSLLQDAGLDASQAGTNLATALTRITNPAEKTTKLMAALGIKFEDTKGNM
                     KSAAEIMAEMTKALAKSKGNYKQAATFAQIFGIDSQKAIINLENMFKTGRAQGLIKEL
                     TTTTDAADTMAKLRMDTFLGDWEQLGGAIDTLKINMFSLNSGALRELVQGTTEWLNVN
                     GAAYAQLVGMKFGELMKEWVPLAIDFGRGMIAGGRELQLWARWAGELIPPLGWLFGDG
                     TKAENAMKLGKAVVVVGGAFFAARVVVKGAEAAMWAFELAAKTVHGTTWLVTWATRAM
                     SGATLASTANLGAAKVAAFATAGGLGALALAAGAAALAVGAVYMANEELKKQTQGMGI
                     LDIASDMWTQGTWDPFKVVDRHQNELARAKRDAERNPDSRLANKASMVPQFDVGSIEQ
                     LTKMVDQQANAASVTTTNLDEFAKSLEQVNTMSLGSDTGAPPQMISPQERTASRIEER
                     QTTVKSELTIRDETGRAEMTKEPPPGAPLGVRLNRSGDL"
     gene            3104..4510
                     /locus_tag="VFQ61_06280"
     CDS             3104..4510
                     /locus_tag="VFQ61_06280"
                     /inference="COORDINATES: protein motif:HMM:NF018818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HET9954089.1"
                     /translation="MVASIATGAAGLLGLGLGVEPQESWEDRLSEAAYTSPVSRTRIT
                     FKYESVSREYEKRGTAFEFAGINDAYVQENGYSARRYPLKCIFTGNNCDRLASSFEAL
                     LLETGVGRLEHPLYGALDVLPFGGITRKDDLKSEANQSVVEVTFWTTTGAAYPQAQSH
                     PQSEILALIADFDAEAAQQFVALTDFRSAVQKANALASLRKRLKDVSATLEAVSEKVA
                     SVSQKFQDAQAALNEGMDSLIGKPLLLAQQITNLVRAPARALSGIADRLDQYGAFADR
                     IMKSFASAPGETFTSGQELTDAVAGRLANDFHITELFALAAVSACVVSLVSDSISDGG
                     EAPVVFASRTQAVEAAEALLSLSDAVSTWREGAYAALETAPSLAAYKYDGGAATQALQ
                     TAVASTAGYLIQLSFALAPERVLELERETTILNLAAELYGEIDEQLDFIIDTNELTGE
                     EILELPRGRRIVHYSPGT"
     gene            4485..5543
                     /locus_tag="VFQ61_06285"
     CDS             4485..5543
                     /locus_tag="VFQ61_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954090.1"
                     /translation="MFTTRPGPDEISLVVDGVDFRHWTEIELTFSMDSFSTANFVAPF
                     EPNDRDHRETFEPFTYKRMRVLLGGQALFTGTMVDINPEATAESSTVHASGYALAGVL
                     DDCCAPASTVPHEFHKVGFRAICEYLASPFGITVQVRGDEGAVFPKVKIREDEEIFPF
                     LTELAQQRNFVLTNTPDGMLLVWRSVPTGSPVARLSGDVSPVSEVSASFSPQDYFSEL
                     TGYAGARRGRRGTKYTAQNKWLTGVLRPKSFHLEDTEKAGAKEVVLAKMGRMFANAAS
                     YRVENIPTWRDPQGDLWTPNTTVVLKAPKVMLYRETEMLIRKVTLKQKTKERTAALEV
                     CLPGCFSGEMPSALPWKP"
     gene            5543..6049
                     /locus_tag="VFQ61_06290"
     CDS             5543..6049
                     /locus_tag="VFQ61_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954091.1"
                     /translation="MGRLAEVIAFERAVDEGAHVEQVKVDPGGDAITSADHVAAAGDD
                     APPLPGDYAQLVDSVGAGGEAAVGYNDPRNPGKAKPGEKRIYARSSDGAEVAEIWLKG
                     SGSITVENGAGSWTLADDGTVTINGVTIDPSGNITAPGEVTAKSSVKLSTHMHPTAMG
                     PSGAPTPG"
     gene            6052..6492
                     /locus_tag="VFQ61_06295"
     CDS             6052..6492
                     /locus_tag="VFQ61_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954092.1"
                     /translation="MPLDIGLLQSSLESVAANPPATSPECAHAWAEAVRSYAEGIVPP
                     SAAVAGAAATLEGALASAFASPAAAPAMEAAFASFAATVGGGMAGFAPTPPAAPVGFA
                     QLFAGPKPATHAAAASALAALIDTWLRLGIATLVAPPNTPTPWS"
     gene            6494..6958
                     /locus_tag="VFQ61_06300"
     CDS             6494..6958
                     /locus_tag="VFQ61_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954093.1"
                     /translation="MDVRLTFTLDGAEISVLGGDIVTDDGLETAVMLSLLGGNEQDSG
                     LESDKRRQWWGNFIEADPARRYRSETLYLLNSLPLIPINAKRVAEAAKRDLSWLVERD
                     IARTLATRGSIAGVDRLLLEIALDVNGRALGFRYESVRTGCDVRLTAAETAQ"
     gene            6955..8106
                     /locus_tag="VFQ61_06305"
     CDS             6955..8106
                     /locus_tag="VFQ61_06305"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HET9954094.1"
                     /translation="MNTPTTQSIASSLLAQLEGQLEHSFLPKSFTDILAKAIAGTYVL
                     VYKYAGFILLQQFVAYATDKPTRINGKVIRPLVEWGRLVGAGDPKPATRAELQVLAVV
                     QSQSGDIPAGAQLTRTEKGVIYTVVASVALNAPTVKLIIRASSDPSGGGGAGTIGNLV
                     AGDTVDFVNPRGVEGKATVLAVLTQGADAEQTEAYRARVIRHYKRKAKGGAPADYQDW
                     AEEVPGVARAIPVAAAQPNEVNVYVRADNNTGVPSAALLNAVKESIQKPKDGRATRRP
                     INAAVNVLGVTRRLFDVRIIGLSPDTPAIREKLKAGIAEYLSTREPYVEGLSTLPRND
                     RITQAALNGIADAIVAADGAQLTSLVLLPGPSYVLQPHEIAALGSTVYE"
     gene            8119..8895
                     /locus_tag="VFQ61_06310"
     CDS             8119..8895
                     /locus_tag="VFQ61_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954095.1"
                     /translation="MALNPNSKYPGQTLIASGWPFGKARNEVSDNDGTGTPFEEDWVN
                     DLWGFQQTLLATAGMTPNGNPDRVGASQYLDALRAIIAADLASAGDPVVIGGAGLEVR
                     AASGFYEPITVEPTDAPVSLLVKQDAQINRWLYAKKIEIGDGTINGQGLLRARLDCSN
                     LGRVVHRSQTLDAAAHIYPAQNQWFWVNPAISGIRTYTINETDCVDDDSFGISNPSTS
                     YYTTIVKPSGPPAVTVPANSWASFKRISGTWQKVSGGSLA"
     gene            8918..9331
                     /locus_tag="VFQ61_06315"
     CDS             8918..9331
                     /locus_tag="VFQ61_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954096.1"
                     /translation="MRTIKLLEPITIREIDGSPALIHGQPLTVSMVHFLASRLRDVVF
                     GQSLETLEHCKAIGDALVSASPSLQELTITDPAWRLLAHAVRTPGLKTQYDPLVGVNL
                     LPFMRAVMTAELSDQLPVNEPQPEQLPVTEPQAPN"
     gene            9346..10134
                     /locus_tag="VFQ61_06320"
     CDS             9346..10134
                     /locus_tag="VFQ61_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954097.1"
                     /translation="MVDFFRQLRHLLPDSVLWRPKDVAEPDWQIGDEHTIGEPGLTIG
                     PHATTRGLQLWRMLKALATVPERAVDFIDGVFADLMPDTTRQLSEWEQQFGILPEGEE
                     ADRRLQVAAAWPPAAGLDPYSLQVLLQAAGFPLYVHEWWTAPGPPWTERDPRLLTEVP
                     IAGSVRCRAASENGPRCTSQHRYRCDGFLVNEPGYLVNLDLTRRAPPAIPDTPSSWRG
                     IFYVGAETFPGRALVPASRRRALERLVLANRPQQYWVVMLVQYY"
     gene            10169..13957
                     /locus_tag="VFQ61_06325"
     CDS             10169..13957
                     /locus_tag="VFQ61_06325"
                     /inference="COORDINATES: protein motif:HMM:NF039277.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954098.1"
                     /translation="MASPKTLTGTPVFSATVLAPGQGEKGNEDTFKRALQTLINNDVA
                     LKAGLGSGTGEGGITTVFSLLADGLVPHPGAEFSGSNAFLRYDGTWAPPIEPATQGSI
                     AVSSTDRYVPFAGARFSGTQDHWDLLATNSTGDQEIFSFDTTAGSSDARLGHSSLATL
                     ELAAKTAILTAGLALKGTHTDTAVPASVTVYNPAAWRTATLARLASAAGGSSIAGFGG
                     GYVVGAASVKVLVNVSVGFNLTILHNAGGAGSDRVVTSTGQALVLGPGESCLLVYDEP
                     LSHWRAFPLVGGSAAAENPSFTDVLASGSSFGGVFDFIHAGNTQGIQALSEASSGNAF
                     GLSFSAQSSDAAHNGEGGKVEIRAGAGGGNREGGFAIVSAGDGGATGAGGQIHLIAGL
                     ANAGTGERADINVFGSRIGNLGEPYAPSHAANKAYVDARSPIASPVRVTPAESPYAAA
                     HGAFVLLVRTALGAVTIDLPQPPEAGDRYIIKDVDGQAAAHPITINHAGLLSDAALSA
                     TVIALPWQSAEIVFESLVAKYLVLSEANAESTSGSSPSLFSSSAPGIVPASGGGAETY
                     LRADGVWAVPVGTGGEGGTVGLFDDTHSGIVPASGGGGAKFLRADRTWMEPPVFSLLN
                     AGYVPASSGDPATFLRADGTWAVPAGSGGLADNSVTLAKLEDIASGRLLGRWSAGTGD
                     PEAITLGGGLEFTGGGALQRTALTGDVTAAAGNSATSIAANAVTSGKILDGAVGTTKI
                     ADLGVTTQKLADDAVTFAKLQNLATDRLVGRDTTGTGDAESLTVGGGIEFTGAGGIQR
                     SALTGAVTAAAGSNTTSISAGAVLSTHIGAGAVLFGNIQSINTDKLVGRDTAGTGVVE
                     EIGLTGGLEFTGTSNIQISAKGVTDAKLRDSAACSVIGRSANTVGSPADITATVDDRF
                     LGRQGGTLGYYQVTTGCIGNDQVTFAKVQNISPGVLLGRSSSFGTGAGDIQEISLGAS
                     LGLEGGQLAVTGLGLSQVMSVSPTTPGTGGSLAIVYSDAIEFWGSEAESYTHESSWLR
                     REDGFLLLSASDALSASGASGGNLILRPGAGDGAGFQGYISVENAKIRNVGSPAAGTD
                     ATNKTYVDGRTLGDVLTASPSGAGGARIPNVGTPTATTDAATKAYVDGKLPTFTAAGQ
                     VPVYDGADLVASGVKTATIADADVATFNPGAASRFVIPTTTANRTITLVSTGMSSLID
                     SGKDWICTFERQNTSAFTVTLVWGSNSWVFPASAPDKLFMQIGWDGTNFFKCGAGALV
                     "
     gene            13954..14958
                     /locus_tag="VFQ61_06330"
     CDS             13954..14958
                     /locus_tag="VFQ61_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954099.1"
                     /translation="MIPIGLRELSDPSTPRVIVRPYESWERLWRAEEAHEYFEEACRR
                     RHEFGLTVADFRALNGWIDSIYNLPAGGAYSNGVPRGRLLNPRNFFGSESSGKALRSW
                     HRADLGYTVATGVSQLLDQSANFYTLTQATATNQPVTSATGLNGRFCLSFDASNDYLS
                     YPSGFAATVAGGNDKPFASWSVARFSTDVLTTTKYSYLWGFGYSGGNSPYIEFYGGYT
                     QTAWVCSKCSNTNVNVSTAGTSPGVAANTNYFIEYYHTGTTSEVIVNGVSCTMSNGGA
                     LDAAQADVNTAAIHALPRSTVVSSNAALLWGEQVFASGKPTALHRFLMTTYKTRQWGF
                     "
     gene            14962..15357
                     /locus_tag="VFQ61_06335"
     CDS             14962..15357
                     /locus_tag="VFQ61_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954100.1"
                     /translation="MAAAQTFTWFYSEDEAQARRCHLLINRAAGYTDEQISEGTQMTG
                     ILGPGERPDQPGFGVAVGAGPDGIAVQDVGAMLVQDVNNRLQDVATPEECAEVQSRLT
                     PEELTEVRSYLQAAAPRGDSWYYIPMREE"
     gene            15560..15787
                     /locus_tag="VFQ61_06340"
     CDS             15560..15787
                     /locus_tag="VFQ61_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954101.1"
                     /translation="MAGLSMSAVAQALRIARAVVKLAPGALDIFEELAEALSSKPAAE
                     QERLAGVIATEIRRRSKEADDAIEFIRTGKT"
     gene            15784..16086
                     /locus_tag="VFQ61_06345"
     CDS             15784..16086
                     /locus_tag="VFQ61_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954102.1"
                     /translation="MSIDPTQLRNILNSLAIVFGSLSTALVLSDASPVLALAAGSIGA
                     FLSGWANLTKPGDVKVPRGSIVTAVSDEESGTQLIAVTPPAAQPKAADTEPPPTGT"
     gene            16097..16897
                     /locus_tag="VFQ61_06350"
     CDS             16097..16897
                     /locus_tag="VFQ61_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HET9954103.1"
                     /translation="MHITHVEARTIVTLALRQRYGREPSRPVRQLAQAVGWLETGYAR
                     HWKPPGDASNNWGAIQAHAGWPIEQTFQYTDTKPNDDGTSTAYVQAFRRYGTPLEGAL
                     DLVKVVYSGGRPLPPNTRYRQELVLPAAERGDLFAFSAGLYDTVYYEGFGRTRAERIG
                     HHALAVRNACAAACRETREPMPDGTDVPPVIRVLREGARGPAISVVQRIVGAPVDESY
                     GPVTALAVKRWQKAHRLKDDGVWGPVCYQVVEREITGEDLDALDGYAA"
     gene            16894..17526
                     /locus_tag="VFQ61_06355"
     CDS             16894..17526
                     /locus_tag="VFQ61_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9954104.1"
                     /translation="MNDEEREATTPRAPANALPHREPEDDPITVSHPLMRYLLSKLDT
                     IERQLEDMRSGAQSIASAVTAIHELQMSSGQVRVDDVVVLLVDDEWLLRSTLTRVIKA
                     SGGTVLSAPSVVEAENLLAREHVDVAVVDIRLSDGSNGFVLAERLVEGKANVGVVLMT
                     GLLLEPDRVAAERLGIRVLEKPVSNETFVQAVKEAYLAKQARVERTSGGQ"
     gene            17523..17792
                     /locus_tag="VFQ61_06360"
     CDS             17523..17792
                     /locus_tag="VFQ61_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954105.1"
                     /translation="MTSLPLEAVGPVVLSLAAAVAALWRQLIVERKDHAQEIQAERAR
                     QDQVEAELRAQLKQQIREQVQYTEFYLRSLEARRPKSLPPEGQTP"
     gene            17789..17932
                     /locus_tag="VFQ61_06365"
     CDS             17789..17932
                     /locus_tag="VFQ61_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954106.1"
                     /translation="MIEFEDAWDEESPTKVLVEKLEANKRDLARIYRESLLPPPMPPD
                     SSK"
     gene            18163..18687
                     /locus_tag="VFQ61_06370"
     CDS             18163..18687
                     /locus_tag="VFQ61_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954107.1"
                     /translation="MAFREVLCACGSSFVPRCEACGDALDEGHLLACNECLELFVVGS
                     DRRVRPVTFIEFELLSPGEVDRYLSSAGADVTQLRGTAVFKRYMRLVEQLHAACVSWC
                     RSNPRAALILNEPTAYFPADLKEMIPLLAQGDADTLDFLLAIDKATNHMATLSQFQVV
                     VSRVKRPLTRGGSA"
     gene            18699..19610
                     /locus_tag="VFQ61_06375"
     CDS             18699..19610
                     /locus_tag="VFQ61_06375"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9954108.1"
                     /translation="MMSERSTTSQDKFEEARINDKVRVKIATPRGREPVWADAVLKDK
                     NRGGARCRTSLGNESFFPWGDIILQPREQQQKKALATLGEIVATHKPVVLRAVPPTEP
                     AASAAEEAAAASDQERIASPPAPPVSTEQSTTPAEREKRGRARLRHELTPIANAIRTH
                     RVRLGMRQIEVAERINTSAQRIGAIELGKILPTDADLEGFAKLAGVPAQDLKAIRDGV
                     PFARPAAAFVEPVRAEPPPPPPPPPPPPPPPPPPPPAAAQTFTAKQQEQFAHFAEALC
                     DVAPIPDDRGKRTLWFALARKLFEVSL"
     gene            19607..20326
                     /locus_tag="VFQ61_06380"
     CDS             19607..20326
                     /locus_tag="VFQ61_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954109.1"
                     /translation="MSALQESAEQESAEQRRVILDAMDLEIDQFYVQAQAIGFHQFIE
                     FAGFMREFVKLCRELEARGKDFRWWAVEPQPYHASYLGEKFGCLYGKMSPEAALKFCE
                     AAFGANASELQRLVDEEEERRWRWVLSELAQYVPPGYNPLRHDDPGLWSPAMYQHLMW
                     LTTEGARSGELRGKSAKAMRWACWAQGALCALGHASLDQCKRLNMRKTWDEARQRQIT
                     NAIFADAAERGVAKKGTETCP"
     gene            20317..21096
                     /locus_tag="VFQ61_06385"
     CDS             20317..21096
                     /locus_tag="VFQ61_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954110.1"
                     /translation="MSVTTKLRELQTCLQQLHGLLPVLDQVIPMYEAIEKALPGAASL
                     ASPPAKPAPAIAAAAVAAKPAKLPTKAAAKLEEPKPDEAPYQPQGASGKVWSVLCDAT
                     DWVGPREMLRHPKLRKLPKSSIYSALNRWSAEGRIEKRPNPIQGTSAPFQYRIVRDSK
                     VEGSKPPSAAPAQQPEKKTSKMSADDRLLAALQGTWMTKRDLHRRVSTLSGSKLKVAL
                     ARLVDSGDVEERKSSEGIVQYTAKQLPDSALNLGLSATAAE"
     gene            21319..22161
                     /locus_tag="VFQ61_06390"
     CDS             21319..22161
                     /locus_tag="VFQ61_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954111.1"
                     /translation="MTEQQQQPTAGASEQPAEAAASGGAAGATASAGAPAGGETVASA
                     GGSDGGAPPTSITISGVETVPLRLSAAGGEGGASLPSFTTPGAGLVFVAAEGEAVTVY
                     GSGREVARWDELTEPAWFASDAAQTYELDAGLRPAVSFYAVVDEGEPDAIGSPSRTDM
                     GATLDAALMFSGFRDGLAPTDERVSDWYVFDVPNGSLHATLRPAANMRAALTLYTPGG
                     EEVPGKNAFHGLPGTELTLVARRLEPGSYVLQVHNFGDLPQPSGIGAVPVNAVQPYEL
                     QITE"
     gene            22249..22896
                     /locus_tag="VFQ61_06395"
     CDS             22249..22896
                     /locus_tag="VFQ61_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954112.1"
                     /translation="MSLKRKVLRNAQEQHTAAREKYEADTAKFVAAMKAWLLMPENAG
                     KKPEFRLPPRGVEFFGALNPDLLALNEDARDLLSYVDRETDHQATLNMAIGVIESITG
                     QERPELDQEAEKLVELYARMLLSCEFGGKDKSVCPHCRTVFDARAALVGEDPDVRPIE
                     GSYNVCGSCGELSLFDAAGDLRRLTADEWASVPDGVSEMARLVKTRVARNRSQDQ"
     gene            22916..23386
                     /locus_tag="VFQ61_06400"
     CDS             22916..23386
                     /locus_tag="VFQ61_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HET9954113.1"
                     /translation="MITHSTQSIFASSADTLVNPVNCAGTMGAGLALEFSKRFPRSML
                     ARYIRATKSGELAKAGDFALWENPRGKSVLLFATKGHWRDRSRIVDIHLGLQRLVEQH
                     RACWSGRVKSIAFPRLGCGLGGLSWTVVQPVMESTLNALEGVAVSIHHHPENAP"
     gene            23383..23709
                     /locus_tag="VFQ61_06405"
     CDS             23383..23709
                     /locus_tag="VFQ61_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954114.1"
                     /translation="MSVAEALFLSRPPWAPFHDGVLWWAIRGRSCMVSVERTGARDRG
                     DYVAKIHARGMLALEIDAHDGWPRYYFDAQRCALEIEAWLRRRGQWVGEGEEPPPWGY
                     FGVEQP"
     gene            23847..24188
                     /locus_tag="VFQ61_06410"
     CDS             23847..24188
                     /locus_tag="VFQ61_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954115.1"
                     /translation="MIHLRQLDGAPPPHREFPEASHEFAIFAIDPSCYESIGSGEMKL
                     VLLQPPDCIEQFTVQSDDDAGLLLREAVSAMTHGCISPDIDAREQWQGFVRMISLTVD
                     KLRTLPENCGC"
     gene            complement(25225..25656)
                     /locus_tag="VFQ61_06415"
     CDS             complement(25225..25656)
                     /locus_tag="VFQ61_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954116.1"
                     /translation="MKKHNGARLPPDISERLRRKLVEELLPRFDGNQSELARTLGYSS
                     QAICRAANGYGGSLGMVLDVAKYLNDDPRELLLGEPSAPPKVRSYPGFAEAVDEALRK
                     YPHLTPRLFEKLGDVRIELGADSWLTPEFLFELAVCYRMRP"
     gene            25778..26149
                     /locus_tag="VFQ61_06420"
     CDS             25778..26149
                     /locus_tag="VFQ61_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954117.1"
                     /translation="MSLARADIRVNVCVVAKTTKYQDLILSALGKVPSALCDENEFML
                     FARAQELDDLVADQVEALRYLAEQVVRDASLTEQGFVDGNALSHSSTRAITERRVAIE
                     ERAKALRLLVQVVTGKKEIRS"
     gene            26403..26603
                     /locus_tag="VFQ61_06425"
     CDS             26403..26603
                     /locus_tag="VFQ61_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954118.1"
                     /translation="MTIWFPLEAKRDEAAKAAKGERVTITTKKSNRGFGLTVEPNEGD
                     DIDEAIEILCRIADDHNATHED"
     gene            26688..26888
                     /locus_tag="VFQ61_06430"
     CDS             26688..26888
                     /locus_tag="VFQ61_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954119.1"
                     /translation="MSRTKKAPLVYTGYVPCACRDCFEIAIGLPGEALCSSCEEAGCE
                     CDGGECQSEGAYSELESLEEER"
     gene            26885..27115
                     /locus_tag="VFQ61_06435"
     CDS             26885..27115
                     /locus_tag="VFQ61_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954120.1"
                     /translation="MRTTHFRALNRAHAAAARAHLRFVVRRLTKAGVPTKAPLTEADW
                     KLNAFAERADAEARVAELEKLNPNSKYAVIDL"
     gene            27183..27395
                     /locus_tag="VFQ61_06440"
     CDS             27183..27395
                     /locus_tag="VFQ61_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954121.1"
                     /translation="MASPKVLNQLRSDLDACSAILRELSSRAHQVSTEADLEAFALAA
                     DQMRQCAEQLREGVAKSSAPRLEVLR"
     gene            27500..28021
                     /locus_tag="VFQ61_06445"
     CDS             27500..28021
                     /locus_tag="VFQ61_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954122.1"
                     /translation="MAITIEDLERLVSEAKADDDARRERLVTLIGLYARLIALREPKR
                     FKARATEYCDEPGTGNGSSPPDKSYRNRTGPRLMLLLETQTEDVPDTGSFYHSWHRVT
                     QDPGLYVDAGGDLWGSEESGTGSFSQFPARPGDCNVHVRVDWSRRTASDLTTADLEAA
                     EAKLRNIAATVLK"
     gene            28018..28287
                     /locus_tag="VFQ61_06450"
     CDS             28018..28287
                     /locus_tag="VFQ61_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954123.1"
                     /translation="MSAKEKSLAPPAAAAAPDAPKPAKAKRSKGGKSSAVTASERAQQ
                     RITELLAAVQARAEQIMQAQRQKPDRWQHFASLAELEDRLRRVLD"
     gene            28351..28692
                     /locus_tag="VFQ61_06455"
     CDS             28351..28692
                     /locus_tag="VFQ61_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954124.1"
                     /translation="MMSQDQPKKKKSGPSGPSGAHQAARRPVQGSEAQWAAWDAKAAR
                     AGLSWAEWVRQLLDEAPGAPVRSWPSSEGPYPGRRLVSASDQQLRAWTRKASAAALSW
                     AEWVRRVQDAA"
     gene            28863..29477
                     /locus_tag="VFQ61_06460"
     CDS             28863..29477
                     /locus_tag="VFQ61_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9954125.1"
                     /translation="MLISTARVIDRELYRSASTTHVRSAQPIELLEELAVLQAAREAL
                     PEVAVYSRVQLTCLSRCGFCAGVKQRKGRPPPPAEELAEQIARRVRWLREMKGLKVKE
                     IEQRAGLSHSAVSRVERGLRGALLPLDTVVRLAWALEVDLAVLIFGNPAGEAPADGSW
                     PESVWLPPELPDPKRGRPAKEQRRPPVSAQQPMRERGGLAKRSK"
     gene            complement(29469..29873)
                     /locus_tag="VFQ61_06465"
     CDS             complement(29469..29873)
                     /locus_tag="VFQ61_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954126.1"
                     /translation="MKKSVLDMSPGERDRIARILEIHSPRVLPAGWFCIERYENASTF
                     SCNDGLRVIVEAEQHGAALWLHVSLSRPDRIPSYEDQTRVKTLFIGRRRTAIQVFPPE
                     AKHVNVHPYCLHLWSPIDHEPLPDFRSAEGLL"
     gene            complement(29870..30136)
                     /locus_tag="VFQ61_06470"
     CDS             complement(29870..30136)
                     /locus_tag="VFQ61_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954127.1"
                     /translation="MHAKARVDRVELALIEVLGALAGLIERGSEAPVEELRGIVTRAL
                     GEIDLSWEGLTAYARVREKLSTLPNNDSRVRVLRAVAILLGVEE"
     gene            complement(30346..30558)
                     /locus_tag="VFQ61_06475"
     CDS             complement(30346..30558)
                     /locus_tag="VFQ61_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954128.1"
                     /translation="MSTFRDLGLPINAPPVVDEATPELAPEVQAIVTAACQWADQRPY
                     CMCAADLSLLQAVAKFREAKRAKKST"
     gene            complement(30555..31565)
                     /locus_tag="VFQ61_06480"
     CDS             complement(30555..31565)
                     /locus_tag="VFQ61_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9954129.1"
                     /translation="MGARKFEDRKATRGRTPLLIGIVGPSGSGKTYSALRLATGMQRV
                     VGGEIFLTDTESGRAEQYADDFTFRHTPFEAPYSPADYLSIFEYCQHRKASIVICDSM
                     SHEHEGIGGVLEMHEAEVRRMAGDDYKKAEKIKLLAWSKPKQERQRLKNAILTRMGNM
                     HFIFCFRAKEKIKLEKGEKEPIPLGWMPISGDDYIYEMTLNMLLYPGSDGVPTWKTDM
                     PGERAIMKLPAQFRNLFARSAPLSEDIGQALASWASGARSGAFEAMASRIANAPTPAA
                     LEQLVPELKGLKVSPAELKLLREAYGERKKALEGYQLGEDEQEPEPPIDDDEPPLQEA
                     AL"
     gene            complement(31546..32448)
                     /locus_tag="VFQ61_06485"
     CDS             complement(31546..32448)
                     /locus_tag="VFQ61_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HET9954130.1"
                     /translation="MGERGTNKPKPNAVVFGLPAAVYHSDPCEVPSLSSSVANILVSR
                     SPLHAWHAHPRLGGRPYEPSASMDAGSVAHAVLLGTAQEELVVVDAEDWRTKAAQEAR
                     AAAREQGKCAVLPATLAEITASVDVIRKRFARYGVRFTGRSEVSLFWTEQASDGTIIQ
                     CRGRIDHLILKDGRARILDLKTARSAHPRACQSHAYEYGYDIQAAAYRSAFAKLYPRY
                     SGREEFLWLFAELDGPNAVAPYALDGSFDWLGERRWRRAVDLWARCLREDSWPSYTDE
                     ILRLEAPQYAVALEEEEGYGSSQI"
     gene            complement(32768..33028)
                     /locus_tag="VFQ61_06490"
     CDS             complement(32768..33028)
                     /locus_tag="VFQ61_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9954131.1"
                     /translation="MKRVGLRIAELRRKRGLTQLQLGKRLKPGSRKAPENLTKEISSY
                     ERGRNFTIRSLAKIANALEYRVRDLFDDPRPSKVRKPGRPPR"
     gene            34370..34903
                     /locus_tag="VFQ61_06495"
     CDS             34370..34903
                     /locus_tag="VFQ61_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954132.1"
                     /translation="MIREWVLAALLALPVSKYDRAADPTLKRAQLETIADAISDAATT
                     ARWSGPPRELAALLIATAYHESSFALHVHGGQCRPHECDGGRARGLWQQQERSTSSRL
                     AWLLLAGLDAEATRLAAHEAARALIRARGTCRSFEREGADWLPMTFSAYAGRGCKAAF
                     PGQSSRVNAFRRLVELW"
     gene            34912..35211
                     /locus_tag="VFQ61_06500"
     CDS             34912..35211
                     /locus_tag="VFQ61_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9954133.1"
                     /translation="MRHARLAELYRLVASEHERLAKESDDEYVDQRHSPLGPRRHCEL
                     VRTGELPGYRGSRKRILVRRADIDSYLERNRILPTETKSAPESFEEVLANYQRAH"
     gene            35239..36822
                     /locus_tag="VFQ61_06505"
     CDS             35239..36822
                     /locus_tag="VFQ61_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9954134.1"
                     /translation="MGRKRKGTAVRKRNTWYAQLTVDFLDGESGLKAFRLDNVRTEAE
                     ANAAVARLQKAVQGKRFPRKGGQLGIAVTFDVYLERWLEARLQRGRRTVHKDRQRLTD
                     YVLPLLKGKPMKLITANDIRGVVNALDLRVSDPTVKFGWKTAHLTWQALTKLFKDATH
                     GKNPALLVLDTNPCLGLSGPDKGTQPTKQWLYPREFVQLISCEEVPLDWRQAYAVATY
                     LYLRIGELRALHCEDVDLASGMVKVHRSTGEDGKTVRLRTKTGAGREFALNPAVVPLL
                     RHLMAKAGGRGRIFPRLDKAAKRLRAHLKRAGVRRESLHNSSPHSQQMTFHDLRGTGI
                     TWAALRGDRLIQIRDQAGHADIEMTNHYMGRASSAGDVGQPFPSLSVLYPATGPTVHS
                     TRPITRFKGVIGDSVMYSVSYPVEPRGIEFPGEAEKADKIPVFASELCPNCRELTRAR
                     DIARDSKLGECRPSSVAATAGVLALLEALERLLGPADSLSEYRVRAGLPPSSAEPIAC
                     VAFTATSCVATPPQAQRGA"
     gene            37273..37884
                     /locus_tag="VFQ61_06510"
     CDS             37273..37884
                     /locus_tag="VFQ61_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954135.1"
                     /translation="MTARITVPATREISLPLPFSAQMLRAVRAHQKTQTRRVVRGQLK
                     LVSGRTLFESASPSWEADDEEVLALWPQKLTCPYPVGATLWVKEGVEKRNGRAVYSAD
                     GAPVKQLKTWRWKRDFLPAMFCPRELSRIHLEVTGRRIERLTNITEADALAEGIVPSP
                     GYPAVPAYAQLWDQLNADRGYSWHVNPWVWVVHFKLIEARRAA"
     gene            37881..38087
                     /locus_tag="VFQ61_06515"
     CDS             37881..38087
                     /locus_tag="VFQ61_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954136.1"
                     /translation="MTTVLSRKKCLSCGAEIVWVEMQSGRRMPLDAKPQKLIVIEADK
                     GEVKLCFTSHFATCPDAAEHRRKP"
     gene            38084..38692
                     /locus_tag="VFQ61_06520"
     CDS             38084..38692
                     /locus_tag="VFQ61_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954137.1"
                     /translation="MTQQQKPPEESATVVQHPTALRPKTDLPFELIVNRGGPVCYHDR
                     RIVDSTLREVRCAACKASLDPIAVLTSIAQDHERYSRALKNARTECNAAEARIQLLKR
                     LEANARARARRAGVSASKSHLDALVRFMSEHLQGHKVDESTVDRLLDGTTPLTRIEAA
                     AQALKRGRAGAAIELVRRAADDLEASLEVAQQALKHVGGRRG"
     gene            38685..38939
                     /locus_tag="VFQ61_06525"
     CDS             38685..38939
                     /locus_tag="VFQ61_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954138.1"
                     /translation="MDECSKLEDLILERVRDWFARAVPPLARQTHTHARLEIRIGDAS
                     ASEDGSLYLQLGVSEELEGPLFDSDSGGAVPGVTFKWGRE"
     gene            38936..39385
                     /locus_tag="VFQ61_06530"
     CDS             38936..39385
                     /locus_tag="VFQ61_06530"
                     /inference="COORDINATES: protein motif:HMM:NF045478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HET9954139.1"
                     /translation="MSPLELVPCTLVEAHEYVRQHHRHLKPAQGALFAVACAADGVVR
                     GVAVVGRPLARMLRDGWTAEIVRCATDGARNACSLLYAAAWRACRALGYRRLVTYTLP
                     SEGGASLRGAGWSLVAQTRGGSWSRRARPRVDRHPTQAKFRWEVVAP"
     gene            39382..40053
                     /locus_tag="VFQ61_06535"
     CDS             39382..40053
                     /locus_tag="VFQ61_06535"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HET9954140.1"
                     /translation="MMKGFSVGLLPGTVTPTSGAHAGTPMLPDSALAQAIVSASRVAE
                     SAAQLHEEQEQAVKRRRRMPEQRPSESKQDYATPRALIRAVEHRFGPIAWDLAAHKDN
                     AVCDLWIGEQQDSLGVRWASELGGLLWLNPPFDRIEDFARKCAREAREGARILMLTPA
                     SVGANWFWDYCSPSRDALVLALTPRLTFEGETDPYPKDCVVSAYGFGTVGFERWQWRQ
                     PRRAL"
     gene            41020..42468
                     /locus_tag="VFQ61_06540"
     CDS             41020..42468
                     /locus_tag="VFQ61_06540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET9954141.1"
                     /translation="MAEKLLPRCDLDAEAAVLSAALLSQDAALEVAEILRADDFFGDA
                     NRHVFNAIVAVLAENGACDIVAVQARLRATGRLEQVGGSPYLAQLADATPAIANVAEH
                     ARVVRNLARVRRAGAVFALLAAEAKAAPIEDFDAWLESCEARACAATAERGDGPATAS
                     DYRELAADAYEQFSAASKAGKPMLGRAMGFHNVDQHLGGVEPGDLVVIAGRPGMGKTA
                     YALQVAETISSDARDKGLAVIFSLEMNRKRLIVRSISRVVGVAANSLRAGKVADWSAV
                     SAGVATLGTLPVVVDDDPSLTPMRLRAKLRRHVAGLRRSFPGYPLACVVVDYIQLMNV
                     DNARKQSTRAEEISQITRSLKQIAKEFNCTVFALSQFRRLDPGKAAPRPQLHDLRESG
                     SIEQDADVVLALHREDYYRPPEKQTHDGRTEVLVLKGRNSGEAVHLIKFHGRTTSFSD
                     DFEPRKQLDLINGANGNGQHYDDSEQYEARYP"
     gene            42419..43012
                     /locus_tag="VFQ61_06545"
     CDS             42419..43012
                     /locus_tag="VFQ61_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954142.1"
                     /translation="MGSTTTTQNNTKRATPEVKIEKFSRNLRVELTEAEINERAQRAA
                     HLLQERDRKDEENKAASKHAKAQVAEIEAELRRVSSEIRDGATYKEVACERRHNYRLG
                     NIQEVRMDTKDVIAERAMNDRERQLDLTLNGSNGASNGSNGNGASAHAEEGDEQPNSV
                     QVLEASEGGAENDSEYEPEPPLETQKPQRARRSRKKP"
     gene            43009..43344
                     /locus_tag="VFQ61_06550"
     CDS             43009..43344
                     /locus_tag="VFQ61_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954143.1"
                     /translation="MSSFEGWAILELMGHRRLAGRVQEVEQFGAKMCRIDVPGAEADQ
                     ATQFYNGSAVYCLTPTTEETARRFAQVNRPGPVALWELLPPVKAEPRDLDTSDDLSST
                     DADDDTNNW"
     gene            43350..43916
                     /locus_tag="VFQ61_06555"
     CDS             43350..43916
                     /locus_tag="VFQ61_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954144.1"
                     /translation="MAFVDVVLGIDLSLTGLGLVAVPADWDLLWSRVHRISLGVKLCD
                     DASPRDHVMRRRALCDDVVRWASRRHVTHAWVEGYPIGRRNLFNLHKQAELRGVLEDR
                     LAELGLYLNPVAEATARKLLLGKLPPTARKEAVFSALDCVGAGFTDRDQADAFTVANY
                     GLKELGAPWVSYGAPEAPQKRARKKRAA"
     gene            43913..44347
                     /locus_tag="VFQ61_06560"
     CDS             43913..44347
                     /locus_tag="VFQ61_06560"
                     /inference="COORDINATES: protein motif:HMM:NF025717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HET9954145.1"
                     /translation="MIAAYMAHPLGAGAQRELNNANALRWFGWLSQSFHVAVSANWLL
                     LASVWPETAEYRARALEIDLELVSRCDEIWLVGGRVSEGMALERAHAARLGKRIVDLT
                     PLGYEPPIWSYQENAEFKQLVEELFERSYAANENNERESGTC"
     gene            44341..44559
                     /locus_tag="VFQ61_06565"
     CDS             44341..44559
                     /locus_tag="VFQ61_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954146.1"
                     /translation="MLMKICRVAVELGERCLLDPFQLDMVNLQLEEQRVTPWIAQYSG
                     NFVIWMCGWLDGATPEEIASAREQNSFV"
     gene            44761..44937
                     /locus_tag="VFQ61_06570"
     CDS             44761..44937
                     /locus_tag="VFQ61_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954147.1"
                     /translation="MTETAEAERRYREDLAFRALADAIARHAHSESYTRHEIMLAAAA
                     AVLLIEQWSHRKPE"
     gene            45152..46762
                     /locus_tag="VFQ61_06575"
     CDS             45152..46762
                     /locus_tag="VFQ61_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9954148.1"
                     /translation="MVEIIKPLDETWSDVGARMRALRKTLPLALNFALRDVFSRAMTQ
                     VEQVANGERADTRWQDDVRKRLRYHWEAQLTRQGAWVAKRSKAKAPMVSDRALAAPGD
                     VLCSETSDYITSRFSGQHFKDLLAGRAGVPTFGGGKSFFSEGRHCSISGSPEKAELSF
                     PLWGTGKRATRFAVAPCGDGHAALWRRLVESADHRVETIDLIKQSKQGDTRKQALLEL
                     ETKRVLKMGRVGIKYNERKGKWFALISWTEYLPDLIEGGLAAACNFGINCFVAALAED
                     GSTYEDVGTDIIATRTRYQHRRRSLQRCVHHRGSGSRGRGVKRREKPLTDLGDAEARW
                     VRDRIRRAAADFASWCVSHGVGVVYLEDLTGLRDSFERATSGTAHEEVKRWIHNWAFY
                     ETQCAFQRQLEESSIVVQRDKARFVSRRCPRKECGHTAAENVKCVGGDWVPVPDHTLQ
                     RLRGMDGEYRVTGGKLYQRQRKQNWFECVNCGFRADGDKVACANHLQDAGREQPLQKA
                     LSAARMVTRVETETASGSVLKASVVAAE"
     repeat_region   46821..47505
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=46821..46856
                     /rpt_unit_seq="ctctcggatccgcggaggaatatcaggtgatcagag"
     gene            47634..48227
                     /locus_tag="VFQ61_06580"
     CDS             47634..48227
                     /locus_tag="VFQ61_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954149.1"
                     /translation="MNRDKDFEEYRAAVARVRELIGNWLNGGVDVFLELQKIDKKGIW
                     RIAYSSFGDFLSREFPNALGLNRYENVIHAIAVYGEKRVRQVGVEGCHALLCAEVVKS
                     EDKRSELEASIDRHVQVTGTPPPPREIRRIVRGILETPSPLAKATISLRRDSALAQEV
                     VLLRAQLREAKKRIRELEAQLRKATGKHTPEGRAAAE"
     gene            48224..49027
                     /locus_tag="VFQ61_06585"
     CDS             48224..49027
                     /locus_tag="VFQ61_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954150.1"
                     /translation="MTAPPLLTAVPRGRVLREADLDWFCSHGQEIFESSAFGAVLERQ
                     ATYGQECAPCERCGGSGFTKDDGTCRSCRGLGGKPRRLRAEASSRRGRLLCGSARCGR
                     CQGRTGLKCDRCGGAGYVDTAPIGSTSERHEEPLAFTPDEQALTRYAQVSRWLIRAGQ
                     WAAVRLQTYYGVPGHRWAGTKWGRVFSLVPLTGAGAKMLGSTANPFSLPSDALLANYV
                     EQLDRMRDATQRMAARSRVAEATRQAVGDFAEACSRWNAAVDASRSRKP"
     gene            49024..49293
                     /locus_tag="VFQ61_06590"
     CDS             49024..49293
                     /locus_tag="VFQ61_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954151.1"
                     /translation="MMAIDSGRRKPDAPLFYTIKDVQQAIGPSWSQRRVRRWLSRAGA
                     LERRYGTVVTTPERLASSFPEVYQRLLRSDEDDDRDDRDDDDEDE"
     gene            complement(49439..50050)
                     /locus_tag="VFQ61_06595"
     CDS             complement(49439..50050)
                     /locus_tag="VFQ61_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954152.1"
                     /translation="MSHSAEGADCRLWAPGAGMDPGMSENVKPLLTLHDRLEAVLAHH
                     GWSARHWSVAAGCREESHVSQLMRTLRREDKPFTGELATWVKLANAAQVSLDWLCFGR
                     NEPEWANAPTKPDPYPSRASVLSVAPTLGFSQAAIADVAGLTLDHDPGFEYWLHELLN
                     REHLRRQQTQQRRRRKRIGQRGSSSKPQRPDEGSAEAEPALDD"
     gene            50107..50907
                     /locus_tag="VFQ61_06600"
     CDS             50107..50907
                     /locus_tag="VFQ61_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954153.1"
                     /translation="MNYRARLAFFGLVILVAACGGESDGGAAVNETGGSTSTSSSGSS
                     STAGTSATAGGTSATTTTSSGGASAAGTDAGGAPQAGGAAPVEAGASGEGGEPVAGAA
                     GSTSAGGATTGGASATGGATTAAGGNTIASGGTAAGGTTATSGGAAAGGTTAAGGTTA
                     QPCECTEGACCDGCHYRPRSYECAHDRVAESSCTGAKTTEVCGGTTAYATSVYRRLEQ
                     QFCSGKSAACDGPSFTTLRIDSPCDLDAVCVDDGTEAKCRLCSDLRWQ"
     gene            51126..52259
                     /locus_tag="VFQ61_06605"
     CDS             51126..52259
                     /locus_tag="VFQ61_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954154.1"
                     /translation="MADRIEVRTILPSKAALFAGVTQCGTILVAPTAEQIAALDSTDR
                     ELLGEILDARRELVVSSPGWEGVLAQLRVERHRKAQAEADLNARKESDRQSLNEVRSL
                     AEQRALFKPGEFPLVQRCYYSLSTYRDPEIDELQKSTDQTVDECRRAFLSAADEELGL
                     SPSSHIVGEGEAATVSEVVRNIANEGRRFKVPTPAAAAIQAAAREIRQTAERVAAEAK
                     ARLAAEQRAELRSIVAQAGTEDQLERFDEGLLPEAELAAVVRSKVFAQIKFPSYAKLK
                     PADVASALECEPSLVKFSTREYDGPLTREQFARLKQIRALAPEGAVVDIREHVAYDSE
                     RTGADDPEVVRLGIRVTAKVGGQKFSAEFAISDDFKPPASKVR"
     gene            52392..52844
                     /locus_tag="VFQ61_06610"
     CDS             52392..52844
                     /locus_tag="VFQ61_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954155.1"
                     /translation="MLRRNVLAELPIAARKSVLLSSHLLVNSSSLRGMTVFALPACEA
                     CLPHVDGGADALLEACRRCPQCGPELQSVLAEGVSQEEIRFYADVARVYRKLVPTVID
                     ENAQVAVHACLEMVTDLTERATGGSPAAVLGALIGMLRDVRARLVGEN"
     gene            complement(52918..53286)
                     /locus_tag="VFQ61_06615"
     CDS             complement(52918..53286)
                     /locus_tag="VFQ61_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954156.1"
                     /translation="MALEPHPRARCADPHLEAGKLRPLGAIPGAPRLSVGHRKTCCAA
                     VSLGDLDELLVTVVARKLQAATPAEVSPERVGHKRCEAVRETPLDSLMVHPISVAHED
                     DALRLALEVLAEVTRDDAPQ"
     gene            53285..53503
                     /locus_tag="VFQ61_06620"
     CDS             53285..53503
                     /locus_tag="VFQ61_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954157.1"
                     /translation="MTDRYNGFSVVLEKDVRSDDAERIIDAIRCIRGVLSVTPHVQDW
                     EGHMAEQRAREHYREQLISVLWPERANR"
     gene            53527..53958
                     /locus_tag="VFQ61_06625"
     CDS             53527..53958
                     /locus_tag="VFQ61_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954158.1"
                     /translation="MRLLLEKQCKEQALKLADGLLEGVPFTLLWFDLDTTEGNVAFVG
                     CRISLAGTIRAFETLLASWEGTATRTPFPLRPDECEALMAAVHETIEAPAGIGFALLI
                     GRGDCTAYIAKAEREGVKTLLSKELLPAWRAELDRRKEHQQ"
     gene            53955..54185
                     /locus_tag="VFQ61_06630"
     CDS             53955..54185
                     /locus_tag="VFQ61_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954159.1"
                     /translation="MILLLELSGAVFSLMGLALLYVGAYRFRRATAILREANAVLDDA
                     LERQKRAMVIATEVDKLRTIIERTTDSAQLPS"
     gene            54481..54630
                     /locus_tag="VFQ61_06635"
     CDS             54481..54630
                     /locus_tag="VFQ61_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954160.1"
                     /translation="MRRKRVVLPAQIKQWRERQQPERPRLKASIGERVIAKQMDRVSS
                     MGEAL"
     gene            54627..55328
                     /locus_tag="VFQ61_06640"
     CDS             54627..55328
                     /locus_tag="VFQ61_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954161.1"
                     /translation="MISVDLKDVEKLERDLQLFARKSLPYAASSAANNMAFTARKTWV
                     SEIESTMVLRNRYTLNSLRVEKASPRGTVESIQAVLGSLAPYMAEQESGAAHQIGNRT
                     AIPTSAAAGQQGAKPRTKPVRAPNRLKAIALAPRSHKGPRKQRNAISMRLALAQGKRF
                     VFLELAKGRGLFRITGAKRKPKVQMVWDFSRGSRPIPKNPTLGRTVSSIQGQGLTFYH
                     EALIEQAKRARVLGY"
     gene            55676..55747
                     /locus_tag="VFQ61_06645"
     tRNA            55676..55747
                     /locus_tag="VFQ61_06645"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55708..55710,aa:Met,seq:cat)
     gene            55854..56585
                     /locus_tag="VFQ61_06650"
     CDS             55854..56585
                     /locus_tag="VFQ61_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954162.1"
                     /translation="MKERLYTRTEFARIVGVTKQAIGQACNGKLGEACVGKRINIEHA
                     AAKEYLALHGAKAPKHLDEDSAPAQSKASKRAQTKPAGQPTTAAPSKKQTAAPPQQSS
                     DPVGKGPVRLIYREVNDLNEIADMTLRQIGAAFGTVTGFRDWLEARRKLEELREKELR
                     NAEADGKLISIKLVRTHVFGAIESGNRRLLTDSPKTITRRLYAAARSDVPMEEAEQMV
                     REIISSQLKPVKETAVRVLRDSSAA"
     gene            56619..58016
                     /locus_tag="VFQ61_06655"
     CDS             56619..58016
                     /locus_tag="VFQ61_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HET9954163.1"
                     /translation="MTRKTTAEPSVPASKKLRAAPPILPQSIEPTGSGVAAEWVPLST
                     LRAWARNPRRNEAAIPQVRDSLIRFGWGRPLVVRSANRELIIGHTASAAAAQLAELWA
                     DPKYDERCENDASLRKDRWHPEAIRTAETGFVPVRFKDVSESDAHRLAVADNKLGEIA
                     EWDALGLAALLQEFGEQGAAEAGFSAEEFAKLLGGIEPAKGEDDDPLELPPKGKAISR
                     PGEVYELGPHRLICGDSRNPKVWAKLLGKERLHIVWTDPPYGVDYVGKTKEALKIEND
                     ALTEEQLLELLRASLGAAAAMCMPGAAWYVASPGGPLFHTFGTVLKELGIWRHTLTWV
                     KDVFVMGRCDYHYKHEPIFYGWEPNGKHYWCGARNFDTVLEFERPKRSTEHPTMKPPE
                     LIQHCIRNSSKPGQLVGDGFGGSGSTLIAAAREGRKARLVELDPRYCDVIRRRWTRFA
                     ESGGMTPGAGALAAA"
     gene            58041..60044
                     /locus_tag="VFQ61_06660"
     CDS             58041..60044
                     /locus_tag="VFQ61_06660"
                     /inference="COORDINATES: protein motif:HMM:NF042573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HET9954164.1"
                     /translation="MCSARVADPLETRAQREWLAQQFDALTTEVMVVSPSAWAERKRY
                     LPPQVTSMPGYYRFEVAPYLREIADCMGIDSPIREVSVMKGVQLGLTVGVLENAIGYY
                     IEYVKNAPCMLVTADNELAKLRVDSYITPMLHYSELEHLIKSSDENNARKTGKTDKRI
                     EWVGGGFLIPIGAQNANKLRSVSIQVSLLDEIDAWPERVGKDGDPLRLAMDRTVAYEA
                     SRKILAISTPLIKGQSRIDARYLRGDQRRYFVCCLKCGHAQTLEWRRENAETGEVTGM
                     VWETENGRLVHDSVRYLCEKCSHPHVNDDKTRLLSPENGAEWRPTAIPTNPDHRSYHI
                     SALYSPVGMQSWGACVERYLEAYDVENSRPRDIALYQVWYNNCLGKPYHVRGEQLRFE
                     AVSVHRRHEYRFGEIPNLWTLGHCGGPVLLLTCAVDVHADSLAVAVFGWCRGRRAILV
                     DYHRFEGDTSQLDDAGTWAQLRELIEEREYAADDGKRYRVQLTLIDSGYLADKVYEFA
                     AEYQVGVHPVKGRDQPTKSARIREFSEFTTPMGTVAFHIFVDLYKDRWSAALRRGWEG
                     EGVQPHGHFNAPSDITDQQLRELTVEVKREIIEKASGKRVGFEWFRPSGAANELWDLL
                     VYNNAALDLIAWDVCRNQLGSETVSWQDFYDQAEQNKLFFTTT"
     gene            60054..60314
                     /locus_tag="VFQ61_06665"
     CDS             60054..60314
                     /locus_tag="VFQ61_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954165.1"
                     /translation="MSMLEEDAFIAARIAAIEARILVYEAAALAINNGASSYSFDDGQ
                     TRMSVTRGDLLRLNETIEKLVAQRDMWLARVRGAGAYVRPGF"
     gene            60314..61996
                     /locus_tag="VFQ61_06670"
     CDS             60314..61996
                     /locus_tag="VFQ61_06670"
                     /inference="COORDINATES: protein motif:HMM:NF016991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HET9954166.1"
                     /translation="MFGLFKRKRADVYDSLFGSGAAPLSLPPAGLQAEAEASIPTLEL
                     SALSTAPLNYRVHDGSKFDGGYGATDLLWTDYWTLRQRSAQLFQTNLYARGIIRRLVT
                     NEINTGLHLEATPEEALLGREEDSLADWAEDVENRFALWSEEPTVCDNREQLTFGALQ
                     ALARLEALVCGDVLVVLRQDQRTGLPRVQLISGAAVQTPWPQVNRPGARIVEGVELDS
                     LDRHVAYWIAQEDGTFKRLPAWGEKTGRRLAWLVYGTERRLDEVRGQPLLALVLQSLR
                     EIDRYRDSTQRKATINSLLAVWIKKTSDKPSTRPLTGGGAMRRGVVETQDSTGTAPRT
                     YRIAEHIPGLVLDELQQGEEPQPFSSHGTDERFGTFEEAIVQGIGWALEIPPEILRLT
                     YSSNYSASQAAINEFKMYLNKTRNQWGKAFCQPIYCDWLLSAALLRDIEAPGYLEGWR
                     NPGQYVVVGAWSSADWTGHIKPAVDQSKLVGGYKEMVAEGFITRDRASRELTGTKYSK
                     NVAKLARENAMLAEAIKPLAKEPSEPEPAIEPEEEPETPVRRSPQPVQRKAS"
     gene            62000..63337
                     /locus_tag="VFQ61_06675"
     CDS             62000..63337
                     /locus_tag="VFQ61_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HET9954167.1"
                     /translation="MWLIQDEVAAAMAQAIKAGVCPTAEQQAAFLERGSAELLGAGAD
                     SLPRNMVVAGDTAEIRIDGVLTPKPDIIAFLLGGGNTTYASIQAALARAQTDGAIKRV
                     VLNIDSPGGSMAGLFETLAAIEAFTKPIRVVASRAASAAYAIAAATRGTIEAVSPASE
                     FGSIGVAAKLPLDEDTIDLTSTDAPKKRPDPRTPEGRAVIVEYLDSVHELFVSAIAKG
                     RGVKPETVNQTYGRGATLLAGEAKRLGMIDKIAKPPAVPAAGDERRARAMRESSEPEA
                     TTMDLNTLKTSHPEVYAAAFAEGRAVAAKPRLVEPEPAQVVPIAPAARAPGQEKPMDL
                     NALRTQYPELYASAVREGELKERDRVTAHLNYGTACGDIKLAVTAIKAGDPITQTAIS
                     EYMTAGMNRSDRAARQAETEAAGSVVAGVKPEAADMADTVADLVERELGKKAV"
     gene            63337..63732
                     /locus_tag="VFQ61_06680"
     CDS             63337..63732
                     /locus_tag="VFQ61_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HET9954168.1"
                     /translation="MANIVTTTIPANGEIILHREEFRDELLVFAGAGTVKRGTILARV
                     GGNLVPFVAGGAGGAEVPVAVLATKDDVTATGAGNVSVRALVAGTVNKNRLIIHADGN
                     GVNITNAILDKLRNVSIVAVDVAVLGPIG"
     gene            63794..64888
                     /locus_tag="VFQ61_06685"
     CDS             63794..64888
                     /locus_tag="VFQ61_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HET9954169.1"
                     /translation="MTDASTRRIIASYTQESPAPMFLHGFFRSPPQNYHSTEMVEIDV
                     QRDSDDVAIVIQDIATGARQNELPGYVNKAFAPPIYDEEVTISSYNLMKRDFGESPYD
                     NPQFQAKVVTAAFRGFRLAENKIRRAIELQASQVLQTGKLTLTDAAGNALFALDYGPR
                     AAHFPTVGVTWGADGTTGNPLGDIAALAEVIRTNGRREPKTLIFGSTALTRFLANKAL
                     KEQFANVGLQRLQEVNPQPASRGSGATNYGRVFIGGYFYEIWSYNATYRNPQTGLSTP
                     YVDPDKVIMISDGARLDCSFGAIPRVGPPDPRVGQFLPARITGPDQGIDLSTFAWLTP
                     DGKHLKVSAGTRPLCIPTGIDEFGCLDMTA"
     gene            64927..65325
                     /locus_tag="VFQ61_06690"
     CDS             64927..65325
                     /locus_tag="VFQ61_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954170.1"
                     /translation="MATNRELIDQAEALGKKLGEPVQTQGLSSSALQDLVTDLQKRVD
                     QAAQLRPLANEPPKSREPNKPNDQPTTAERNAGQQAGEQPKSPYLVAKGRSVIGARGV
                     IDEGKEITPEDVGGQENLDRHVAAGNVDKR"
     gene            65325..65711
                     /locus_tag="VFQ61_06695"
     CDS             65325..65711
                     /locus_tag="VFQ61_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954171.1"
                     /translation="MPLWLQAAADAKAILADVGGFARPITITSPAGFTVLVTGTARDI
                     STTIDPATGAVVSARTASITIPIEPLSAAGLSIPRNVPDKTSKPWLAQFYDAAGVLHV
                     FKICESHPDLTLGIVTCLLETYRLAP"
     gene            65718..66395
                     /locus_tag="VFQ61_06700"
     CDS             65718..66395
                     /locus_tag="VFQ61_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954172.1"
                     /translation="MDPQISELLLEADGLVLVRDQIATILAVESAGQMALAAQVGADP
                     MPWYLRVFTERTAPWESYLLEPSGGTSDMGPIVNVSWESTQFDLKHGNVVSAQRGGGL
                     FHIDCYAYAVSADDGLSGHVPGDEQAKREVQRVLGLVRKILMAAHYTYLGLPGRVARR
                     WVTAENALSAAVDDRSLQRVAAARLSLQVEYGEQAPQVTGPPLREVVTSFKRSDTGQL
                     IAVRRNI"
     gene            66409..68190
                     /locus_tag="VFQ61_06705"
     CDS             66409..68190
                     /locus_tag="VFQ61_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954173.1"
                     /translation="MSIDASAVARVTGITTQFQDMRAGSVLFLPQQIAVFAQGNSDAL
                     YSDEKWLAPSAGATGARFGFGSPAHLILRELMPSNGDGVGTIPVRVYPLTAPAGAVAA
                     QGELTVSGTATKGAAYRLRIGGVLSEAFVIPKGAVNAADVYGRISAAVRAVLEMPVNV
                     SHSYGTPTASQGPNEGDGTIGSLSVSGTPSPGTWRLVLTNEVANGGIWSLIDPSGTTV
                     ANDLTMIVGPGATTAFSRAGLNFSVTDGAVNFASGDEFQITVPATSARFTAKWKGASG
                     NAIKVEVLTDEYVGVVFAVTQPTGGLLNPSVTAALEKVGPVWESMAINAAPIEDTTIL
                     DQFREFGEGRWLPEVGKPIVVFTGVTHPTVELATAVSSGRRDDRVNAQLVAPGSPNLP
                     FVVAARQLARIAKIANNDPARDYGSQRATGLIPGPDEDQWGYLQRDQAVKLGSSTIEI
                     KDGVVAISDVVTFYRPTGEEPPAYRFVKNIVKLQTCLYNVWLAFNTEDWDGAPLIPDD
                     QPTVSGSAKKPKTMRALAAAIVDSLGLQAIISDPARAKNGIVAQISTQNPDRLDLEIP
                     VQVSGNTNVKDIRLKWGLYFGTAAVVG"
BASE COUNT        13336 a        20907 c        22340 g        11684 t
ORIGIN      
        1 tttccagctc gaggtgcagg cgaacgggga cggtacagcg cggatcctca agactcgggt
       61 caactggagc atcggcggct tgtcgctggt gatcaacgat gatcgtggag atcaagagtt
      121 cctgcgcaaa gtgcagggga agaacgagtt cgttccgatc acgatcacgt tcgcgtctca
      181 gaacacgtat caaggaagag ggcaagttgt ggacgagctt tctttcagct ccgagtcctc
      241 gactgccgat gtaacgctta cgggcgaagg ggagctgaca aagcaatgaa caatgtggtg
      301 gcactacaga caggcacctc ggcgacgagc ctcgaggtgg ccgaggctga gtttcaacgt
      361 ttcgtggcag ctatggccct cgacgtcgac acgtccacga tggacgagga agaccgagag
      421 gagttcaagg acaagaaacg caagatcgtg cgcgcgctca tgctagggtc gctcgtgatc
      481 gacgagaagg ggcagcccgt ctacacgccg caggtcggag atgaccgaag ccccattacg
      541 ttcttggagc cgcgcggctc ctccctcatg gctatggacc agcgcaagca ggggcacaac
      601 gtggcgaaga gtttcgcggt gctcgctgac atcacgaaag ttagtcccca gcgcttcgcg
      661 aatatgttca accgcgacat ccaagtttgc ttggcgttgc aggccctttt tttgggctag
      721 aggtcacgcc gctcctcgtg cgccatgggg cggacgcgag gctgcccaac ggaacgcaca
      781 cgctcgtgcc ggtctactcg gagatgctca cgcagatctg ccgtgactac tcaggactac
      841 ccgacccgag aactctgacg cttactgaga tccggttctt ctacgaagcg ctacgcgccg
      901 agctccgcca ccacacgaag cccagaccga agaagtgaat catggctggt cgttttagcg
      961 tcgaggcgat cttcaaggcg gtcgaccgcc ttactgcgcc cgttgctcgt atgagcgcga
     1021 gcgtggcgaa gttcgcctcg aacgcgaagg acaagatcag caaaatcgac gacgagttcg
     1081 acaaggtaca gagcacgatc aaagcgggag cggtggcgat cggcgcgggt gcagccgtcg
     1141 cgggactcgg aatcaagtcc gtagtcgata ccggcgcaga gttcgagcag gctatagcgg
     1201 acgtcggagc ggtatcgctg atgaccgccg ctcaggtcgg cgacctccgc tccaagtcgc
     1261 ttgagctcgg tgcggcgacc agatactcag caactcaggt cgcccgcggc tttgagtcaa
     1321 tgggccgcgc gggtttcaat aacgagcaaa tgctgtccgg gatctccggg attctctcgg
     1381 ctgcagccgc ggaaggcgcg gagatcgagg agacagccga cacggtatca gctgtgctta
     1441 agggcatgcg cttggaccag ggagacttgg gagcagaagc caaccgtgtt gctaacgtcc
     1501 tcgcactggc atccgctcgc accaaagcgt cgctcctcag cttgggcgag tccatgaaaa
     1561 cgttgggccc aacagcggcg caattcaata tcccgctcga gaccgctgtg ggcatggtgt
     1621 cgctgttgca agacgcgggg ctcgacgctt ctcaggccgg cacaaacctc gcgactgccc
     1681 ttacgcgcat cacgaatccg gctgagaaga ccaccaagct gatggccgcg ttgggcataa
     1741 agttcgagga taccaaaggc aacatgaaga gcgcggccga gattatggcc gagatgacaa
     1801 aggcactcgc taaatcgaag ggcaattaca agcaggcagc gacgtttgct cagatcttcg
     1861 gtatcgactc gcagaaggcg atcatcaatc tcgagaacat gttcaagacc ggacgagcgc
     1921 agggtctgat caaagaactg acgacgacga cggacgccgc agacacgatg gcaaagctcc
     1981 ggatggacac cttcctcggt gactgggagc agctcggagg cgctatcgat acgctgaaga
     2041 tcaacatgtt ttcgctcaac agcggagctc tgcgagaact cgtgcagggc acgacggaat
     2101 ggctcaacgt caacggcgcg gcctatgctc agctcgtcgg tatgaagttc ggcgagctca
     2161 tgaaggagtg ggtgccgctg gctatagact tcggccgcgg gatgattgcg ggcgggcgag
     2221 aactccagct ctgggcgcgg tgggcgggcg agctaatccc gccgctcggg tggctcttcg
     2281 gagacggcac caaggccgag aacgccatga agctcgggaa ggctgtcgtc gttgttggcg
     2341 gcgcgttctt cgcggctcga gtcgttgtga agggcgcgga ggcagcgatg tgggccttcg
     2401 agctcgcggc caagacggtg cacgggacaa cgtggctcgt gacctgggca acgcgagcca
     2461 tgagcggggc aacgctcgcc tcgactgcca atctcggcgc ggcgaaggtg gctgcgttcg
     2521 cgacagccgg gggactggga gcgctagcgc tagctgctgg tgcggccgcc ctcgcggtgg
     2581 gcgctgtcta catggccaac gaggagctca agaagcagac gcagggcatg gggattcttg
     2641 atattgcgag cgacatgtgg acccagggca cgtgggaccc gttcaaggtt gtggaccgtc
     2701 accagaacga gctcgcccga gcgaagcgcg acgctgagcg caacccggat tctcgtctcg
     2761 caaacaaggc gtcaatggtg ccgcaattcg acgttgggag tatcgagcaa ctcacgaaga
     2821 tggtcgacca gcaggcaaac gccgcaagcg tcaccacaac caacctcgac gagttcgcga
     2881 agtcgctgga gcaggttaat acgatgtcgc tcggcagcga tacgggagcg ccgccgcaaa
     2941 tgatctctcc gcaggagcgc acggcgagcc ggatcgagga gcggcaaacc accgtgaagt
     3001 ctgagctcac gattcgagac gaaactggca gagctgagat gacgaaagag ccgccaccgg
     3061 gcgctccgct cggggtgcgg ctgaaccgca gtggggatct ctgatggtcg cttcgatcgc
     3121 aaccggagcc gctgggcttc tcggacttgg cctcggcgtc gagccgcagg agtcttggga
     3181 ggatcgccta agtgaggcgg cgtatacctc gcccgtcagc cgcacgcgca tcacgttcaa
     3241 gtatgagagc gtcagccgcg agtacgagaa gcgcggaacc gctttcgagt ttgcgggcat
     3301 caacgatgcc tacgtgcagg agaacggata tagcgcacgc cgctacccgc ttaagtgcat
     3361 cttcacgggc aacaattgcg atcgcctcgc ctcatccttc gaggccctgc tactcgagac
     3421 tggcgtgggg cgcttggagc acccgctcta tggcgcgctc gacgttctgc cgtttggcgg
     3481 catcactcgc aaagacgacc tgaagagcga ggccaaccag agcgtcgtcg aggtcacctt
     3541 ttggacgacc acaggcgcgg catacccgca ggcgcagtcg cacccgcaga gtgagatcct
     3601 cgcattgatt gcggacttcg acgcggaagc tgcgcaacaa ttcgttgctt tgacggactt
     3661 ccgcagcgcg gtgcaaaagg caaacgctct cgcatcgctt cggaaacggc tcaaggacgt
     3721 gagcgccacg ctcgaggcgg tgagcgagaa ggtcgcgtcc gtaagccaga agtttcagga
     3781 cgcgcaggcg gcgctgaacg agggaatgga ctcgttgatc gggaagccgc ttctcctcgc
     3841 gcagcagatt acgaatctgg tgcgagcacc ggctcgagcg ttgagcggca ttgcagacag
     3901 gctcgaccaa tacggagcct tcgccgatcg gatcatgaag tctttcgcct cggctcccgg
     3961 ggaaaccttc acctcagggc aggagctgac ggacgcagta gcagggcggc tcgccaatga
     4021 cttccacatc acggagctgt tcgcgcttgc cgctgtctcg gcatgtgtcg tctccctggt
     4081 ttcagactcc atctctgatg gtggcgaggc gcctgttgtt ttcgcttcaa ggacccaggc
     4141 ggtcgaggcg gctgaggcgc tcctgtcgtt atcggatgcg gtttcgactt ggagggaggg
     4201 cgcctatgcg gctctcgaga ccgcgccttc gctggccgcc tacaaatacg atggtggagc
     4261 cgcaacgcag gctctgcaga cggctgtagc gtccaccgcg gggtacctca ttcagctttc
     4321 tttcgcgctc gctccagaac gcgttctcga gctcgagcgc gagaccacca tcctgaatct
     4381 cgcggctgag ctgtacggcg agattgacga gcagctcgac ttcatcatcg acaccaacga
     4441 gctcacgggc gaagagatcc tggagctgcc gagagggcgc cgcattgttc actactcgcc
     4501 cgggacctga tgagatcagt ctcgtcgtcg acggcgtgga cttccggcac tggacggaga
     4561 tcgaactcac gttcagcatg gactccttct cgacagcgaa cttcgtggcg ccgttcgagc
     4621 ctaatgaccg cgaccaccgc gagacgttcg agccgttcac gtacaagcgc atgcgcgtgc
     4681 tgctcggagg ccaagcgctg ttcaccggaa cgatggtcga catcaatccg gaggcgacgg
     4741 cggagtcgag cacagtgcac gcctcgggat atgcactcgc tggagtgctc gacgattgct
     4801 gcgcgcctgc cagcacggtc ccgcatgagt ttcacaaggt cgggttccga gcgatctgcg
     4861 agtatctcgc gtcgcccttc ggcattacgg tccaggtgcg aggcgacgag ggcgcggtgt
     4921 ttcctaaggt gaagatccga gaggacgagg agatcttccc cttccttact gagctcgccc
     4981 agcagcgaaa cttcgtcctg acgaacacgc ccgacggcat gctgctggtt tggcgatccg
     5041 tgccgaccgg cagccctgtt gctcgtctca gcggcgacgt gtctccggtg agtgaggtct
     5101 cggcctcgtt ctcgccgcag gactacttca gtgagcttac cggctatgct ggcgcgcgga
     5161 gaggccgccg gggcacgaag tacacggcac agaacaagtg gcttactggc gtgctccggc
     5221 cgaagtcgtt tcatctcgaa gataccgaga aggcaggcgc gaaggaagtc gtgctcgcaa
     5281 agatgggtcg catgtttgcg aacgccgcct cctaccgagt cgagaacata ccaacatggc
     5341 gagaccccca gggagacctg tggacaccca atacaacggt ggttttgaaa gcacctaagg
     5401 tgatgctgta tcgagagacc gagatgctga ttcggaaagt gacgttgaag cagaagacga
     5461 aggagcgcac cgctgccctc gaggtctgct tgccgggctg cttcagcggc gagatgccga
     5521 gcgcgttacc ctggaaaccc tgatgggaag gctagcagag gtcatcgcat tcgagcgcgc
     5581 ggtcgatgag ggagcgcacg tcgagcaggt caaggtggat cccggcggag acgctatcac
     5641 gtcagccgat cacgtagcgg ctgcaggaga cgacgccccc cctctgcctg gcgattacgc
     5701 ccaactcgtc gactcagtgg gagccggcgg agaggcggca gtcgggtaca acgatccgcg
     5761 caatcccggc aaagcgaaac ctggcgaaaa gcgcatctat gctcgcagct cggacggcgc
     5821 ggaagtcgcc gagatctggc tgaagggcag cgggtcgatc acggtagaaa acggcgcggg
     5881 ctcgtggacg ctggccgacg acggcaccgt cacgatcaac ggcgtaacaa tcgacccgtc
     5941 tggcaacatc acagcgccgg gcgaggtgac cgcgaagagc agcgtgaagc tcagcaccca
     6001 catgcacccg accgcgatgg ggccgagcgg cgcaccgaca ccaggctaac gatgcctttg
     6061 gacattgggc tgctgcagag cagccttgaa agcgtggccg ccaatccgcc ggccacatcg
     6121 cccgagtgcg cgcatgcctg ggcagaagcc gtacggtcct atgcggaggg catcgtcccg
     6181 ccctccgctg ccgtcgctgg cgctgcggct accctcgaag gtgccttggc ctcggctttc
     6241 gcctctccag cggctgcgcc cgctatggag gccgcgttcg cctcgttcgc agcgacggtc
     6301 ggtgggggca tggccggctt cgccccgacc cctcccgctg ccccggtagg attcgcacag
     6361 ctgttcgccg gccccaagcc ggccacacat gcagccgccg catccgcgct cgcagcgctg
     6421 atcgacacct ggttacgcct cggcatagcc accctcgtgg ctcctccgaa cacgccaacg
     6481 ccttggagct gacatggacg tccggctgac cttcaccctg gatggcgcgg agatcagcgt
     6541 tctcggtgga gacatcgtca ctgacgacgg tctcgagacc gcggtgatgc tttcgcttct
     6601 cggaggcaac gagcaggaca gcggtctcga gagcgacaag cgtcggcagt ggtggggcaa
     6661 cttcatcgaa gccgatcccg cccgccgtta caggagcgaa actctatatc tcctgaactc
     6721 gttacctttg ataccgatca acgcgaagcg tgtggccgaa gctgcgaagc gggatctctc
     6781 gtggctggtc gagcgggaca tcgctcgaac tctggctacc cgcggatcca tcgctggcgt
     6841 tgaccggctg ctgctcgaga tcgcactcga cgtgaacgga cgagccctcg ggttccggta
     6901 cgaaagcgtc cgcacgggtt gcgacgtccg gctcaccgca gcggaaacgg cccaatgaac
     6961 acaccgacca cgcagagcat cgcgtcgagt ctcctagcgc agctcgaagg gcagctggag
     7021 cattcgtttc tgcccaagtc gttcaccgat atcctggcaa aggcaatcgc ggggacgtac
     7081 gtcctcgtct acaaatacgc cgggtttatt ctgcttcagc agttcgttgc gtacgcgacg
     7141 gacaaaccga cgcgcataaa cggcaaggtg atccgcccgc tggtggagtg gggccggctc
     7201 gttggagctg gagatccgaa gcccgcaaca cgcgcggagc tccaagtcct ggctgttgtt
     7261 cagagccaga gcggtgacat tccggctggc gctcagctca ctcgaactga aaagggcgtg
     7321 atctatacgg tcgtcgcctc cgtcgcgctg aatgctccaa cggtgaagct gatcatccga
     7381 gcgagctcgg atccatcggg tggaggcggg gcgggcacca tcggcaacct ggtggcgggc
     7441 gacaccgtag acttcgtgaa cccgcgcggc gtggaaggga aggcgacggt ccttgctgtg
     7501 ctgactcagg gagcggacgc cgagcagact gaggcgtatc gcgcgcgcgt catccgtcac
     7561 tacaagcgca aggccaaggg cggtgcgccc gcagactatc aggactgggc cgaggaggtg
     7621 ccgggagtag caagggctat cccggttgca gcggcgcagc cgaacgaggt caacgtctac
     7681 gtcagggcag acaacaacac gggcgtgccc tctgccgcgc tgctgaatgc cgttaaagag
     7741 tccatccaga aaccgaagga cgggcgagcc acgcgccgcc caatcaatgc cgcggtcaac
     7801 gtgcttggcg ttacccgcag gctgttcgac gtgcgcatca ttggattgtc gcctgacact
     7861 ccggcaatca gggagaagct caaggcgggg attgcggagt atctctcgac gcgagaaccc
     7921 tacgtcgaag gcctgtctac tctgccgcgc aacgaccgaa tcactcaggc agcgctgaat
     7981 ggtatcgcag acgcgatcgt agctgcggac ggagctcagc tcaccagctt ggtgttgctg
     8041 ccgggcccct cttacgtgct gcagccgcac gaaatcgcgg cacttggatc aaccgtttac
     8101 gaatgagagg gcgcgcgctt ggctcttaat ccgaactcca agtatccggg tcaaaccctg
     8161 atcgctagcg gctggccatt cggcaaagcg cgcaacgagg tctccgacaa cgacggcacc
     8221 ggcacgccgt tcgaagagga ctgggtcaac gatctctggg gcttccaaca gacgctgctc
     8281 gcgaccgcgg gcatgactcc caatgggaat ccagatcgtg ttggtgcttc ccaatacctc
     8341 gacgcgctaa gagcgattat tgccgccgac ctcgcgagcg ctggtgatcc ggtagtgatc
     8401 ggcggagcgg ggctcgaggt gagagcggcc agcggcttct atgagccgat cacggttgag
     8461 cccaccgatg cgcccgtgtc gctgctcgtg aagcaggacg ctcaaatcaa ccgttggttg
     8521 tacgcgaaga agattgagat cggcgacggc acgatcaacg ggcagggact gctgcgcgct
     8581 cggctcgact gctccaacct cgggcgcgtc gttcaccgat cgcagacgct cgatgctgcc
     8641 gcccatatct atcccgccca gaaccagtgg ttttgggtca acccagcaat ctccgggata
     8701 aggacgtaca ccatcaacga gacggattgc gtcgacgacg acagctttgg aatcagcaat
     8761 ccatcgacca gctactacac gacgatcgtc aaaccatcgg ggccgcctgc ggttaccgtg
     8821 cccgccaact cgtgggcatc gtttaagcgt atctcgggga cttggcaaaa ggtctccggc
     8881 ggctctctcg cttgagaagg aagaaacacg catgctgatg agaacgataa aactactgga
     8941 gccaatcacg atccgagaga tcgacggcag ccccgcgctc attcatggtc agccactcac
     9001 cgtctctatg gtgcacttcc tagcatcacg attgcgcgac gtggtgtttg ggcaatcact
     9061 cgagacgctg gagcactgca aggcgatcgg cgacgcgctc gtctccgcta gtcccagcct
     9121 gcaggagtta acgattaccg atcctgcatg gcgcttgctc gcgcatgccg ttagaacgcc
     9181 cggcctcaag acccaatatg acccgctcgt aggtgtgaac ctcctgcctt tcatgcgggc
     9241 cgtaatgact gcggagctga gcgaccagct gccggttaac gagccacagc cggagcagct
     9301 gcccgtcaca gagccgcaag cgccgaactg aaaaagcacc aacgcatggt cgacttcttc
     9361 cggcagctcc gccacctgtt gcctgactcc gtgctctggc gtcccaagga cgtagcggag
     9421 ccggattggc aaatcgggga cgagcatacg atcggcgagc ctggcctcac gatcgggccg
     9481 cacgcgacga ctcgcgggct gcagctgtgg cgcatgctca aggcgctcgc gacggtgccg
     9541 gagcgagcgg tcgactttat cgacggcgtg ttcgcagact tgatgccgga tacaactcgc
     9601 cagctctccg agtgggaaca gcagttcggg atcttacccg agggcgagga agcagatcgg
     9661 aggctgcaag tagcagcggc gtggccgcct gccgcgggac tcgacccgta ctcgctgcag
     9721 gtgctgctgc aagccgcggg gttccctctc tacgttcacg aatggtggac tgctcctggt
     9781 cctccgtgga ccgagagaga tcctcgattg ctgacggaag tcccgatagc cggttcagtg
     9841 cggtgcagag cagcaagcga gaacgggccg cgctgcacct cgcagcatcg atatcgatgc
     9901 gacggattcc tggtgaacga gccaggctat ctggtcaatc tagacctcac gaggcgagcc
     9961 ccaccagcta ttccggacac gccgagtagc tggcggggca tcttctacgt tggtgcggag
    10021 acattccccg gaagagccct cgtgccagcg agcagaagac gtgcgcttga gcgcctcgtg
    10081 ctcgcaaacc gcccacagca gtattgggtg gtgatgctcg ttcagtacta ctgagctcct
    10141 cctttccaga tccggaaagc aaacaaacat ggcatcaccg aagaccctca cgggtacgcc
    10201 ggtattctct gcgaccgttc tagccccagg gcagggcgag aaaggcaacg aggatacgtt
    10261 taagcgcgcg ctgcagacgc tcatcaacaa cgatgtagcg ctgaaggctg gccttggttc
    10321 aggcacaggc gagggcggaa tcacgaccgt cttctcccta ctcgccgacg gccttgtgcc
    10381 gcacccgggc gcagagttct cgggctcgaa cgcgttcctc cgctacgacg gaacgtgggc
    10441 gccgcctatc gagccagcta cacagggttc catcgcggtc agttccacgg atcgctatgt
    10501 cccgtttgcc ggcgcacgct tcagcggaac gcaggaccat tgggatctgc tcgcgacaaa
    10561 ctcgacgggt gatcaggaga ttttctcgtt cgacaccacc gcgggaagct ccgacgcgcg
    10621 gctcggacat tcgagtctcg ccactctgga gcttgcggcc aagacggcaa tccttaccgc
    10681 cgggctcgcg ctaaagggca cgcatacgga cactgctgtg cccgcgagcg tcacggttta
    10741 caatccggcg gcttggcgca cagcgacgct cgcgcgcctg gcctctgcag caggtggctc
    10801 ctcgattgct gggttcggcg gtggctatgt tgttggcgcg gcctcggtca aggtcctcgt
    10861 aaacgtctcg gtcgggttta atctgaccat tctgcataac gctggcggtg ctggtagcga
    10921 ccgcgtcgtt accagcaccg gacaggcgct cgtgcttggt ccgggcgagt cgtgcttgct
    10981 cgtctacgac gagcctctct cgcactggcg tgcattcccg ctcgtcggcg gcagtgccgc
    11041 ggcagagaac ccctccttta cggatgtgct cgcgtccggt agctcattcg gaggggtgtt
    11101 cgactttatc cacgctggca acacgcaagg gattcaggcg ctcagtgagg cctcatcggg
    11161 taacgcgttt ggcctttcgt tttcggcgca gtcctcggat gccgcgcaca acggcgaggg
    11221 aggcaaggtc gagattcgcg ctggtgcagg ggggggcaat cgcgaaggcg gctttgctat
    11281 tgtcagcgct ggagacggag gcgcaaccgg agcgggcggg cagatccacc tcatcgctgg
    11341 gctggcgaac gccggcaccg gcgagcgtgc ggatatcaac gtctttggct cgcggatagg
    11401 aaatctcggg gaaccgtatg cccccagcca cgcagcgaat aaggcctatg tggacgcacg
    11461 ctcgcctatc gcttccccgg tgcgagttac accggctgaa tcgccctatg cggcagctca
    11521 cggcgcattt gtgctgctcg tgcgcacggc actgggcgcg gtgacgatcg atttgcctca
    11581 gccaccggag gcaggcgatc ggtacataat caaggacgtg gacgggcagg ccgccgccca
    11641 tcccataaca atcaatcacg caggcctgct gtctgatgcg gcgttaagcg cgacggtcat
    11701 cgcgctcccg tggcagtcag cagagatcgt ttttgaaagc ttggtggcga agtatctggt
    11761 gctgtcggag gcgaatgcag agagcacgag cggctcgagt ccatctctgt tctcctctag
    11821 tgctccagga attgtgcccg cctctggcgg tggcgcagaa acatacctgc gcgcggacgg
    11881 cgtctgggct gttcctgtgg gcaccggagg cgaagggggc acggtcgggc ttttcgacga
    11941 tacccattcc ggtatcgtgc cagcctcggg aggcggtggc gccaagtttc tgcgcgcaga
    12001 ccgcacatgg atggaaccgc cggtgttctc tctcctgaac gccggctatg tgcctgcttc
    12061 gagcggcgac ccagcaacat tcctgcgcgc agacggaacg tgggctgtac cggctggctc
    12121 cggcgggttg gctgacaatt cggttacgct ggcgaagctc gaagatatcg cgagtggccg
    12181 tctactcggg cgctggtcgg caggcactgg cgatccggaa gcaatcacac ttggtggtgg
    12241 cctcgagttt accggtggcg gggctctgca acggacggcg ctaactggcg acgtgaccgc
    12301 agctgctggc aactcagcga catcgatcgc tgcaaacgcg gtcacctcag ggaagatcct
    12361 cgacggggca gtaggcacga cgaagatcgc agatctcgga gtaacaaccc agaagctcgc
    12421 cgacgacgcg gtcacgtttg caaaactcca gaacctagcg accgaccgcc tcgttgggcg
    12481 ggatacgacg ggcactggag atgcggagtc tctcacggta ggcggaggta tcgagttcac
    12541 tggagcaggc ggcattcagc ggtctgcgct cacgggagcg gttacagcag cagcaggctc
    12601 gaatacgacg tcgattagtg cgggtgcggt cctctcgacg cacattggag cgggggcagt
    12661 tctcttcggc aatatccaaa gcatcaatac cgacaagcta gtcggccgag atacggcggg
    12721 gacgggcgta gtcgaggaga tcggccttac cggcggcctc gagttcactg gcaccagcaa
    12781 tatccagatc tcggcgaagg gcgttaccga cgcgaagctc cgcgactccg ccgcttgctc
    12841 ggttatcggg cgctcagcga acacggtggg ttctcccgcc gacatcaccg caacggttga
    12901 cgaccggttt ctcgggcggc agggaggcac gctcggctac taccaagtaa caactggatg
    12961 cattggtaac gaccaagtca ccttcgcgaa ggtgcagaac atcagcccgg gagtgttgct
    13021 cgggcgctcg tcgtcgtttg gaactggagc gggcgatatt caggagatct ccctaggcgc
    13081 cagcctgggg cttgagggcg ggcaattggc agttactggg ctcgggctca gccaagtaat
    13141 gtccgttagc cccaccacgc cgggtacagg cgggagcctt gcgatcgtat acagcgatgc
    13201 gatcgagttt tgggggagcg aggccgagag ctacacgcac gaatcttcgt ggctccgtcg
    13261 cgaggatggc ttcctgcttt tgtccgcttc tgatgcattg tccgcgtctg gagcaagcgg
    13321 cggcaacctc atcctgcggc ccggagcggg cgacggcgca ggctttcagg gatacatcag
    13381 tgtcgagaat gccaagatca ggaatgtcgg ctcgcccgca gccggcacgg acgcaacgaa
    13441 caagacatac gtcgacggcc gcacgctggg cgacgtgctc accgcttctc cgagcggagc
    13501 cggcggagcg cggatcccca acgtcggcac gccgacggct accaccgacg ccgcaaccaa
    13561 agcctatgtc gatgggaagc tcccaacgtt cactgcggca ggccaggtgc ccgtctacga
    13621 cggtgcggat ctcgttgcat cgggtgtcaa gacggcgacg atcgcagacg ctgacgtcgc
    13681 gacattcaat ccgggcgcgg cgtcgcgctt cgtaatcccg acgaccaccg cgaacagaac
    13741 catcaccttg gtgagcaccg gcatgtcgag tctcatcgac agcgggaaag actggatctg
    13801 cactttcgaa aggcagaaca cgagcgcctt caccgtgacg ttggtttggg gctccaactc
    13861 ctgggtgttc cccgctagcg cgcctgacaa gctgttcatg cagattggct gggacggcac
    13921 caacttcttc aagtgcggcg cgggagcact cgtatgatcc cgatcggcct gcgggagctc
    13981 tcagacccga gcacgccgcg ggttatcgtg cggccatacg agtcgtggga gcggctatgg
    14041 cgggcggagg aagcgcacga gtacttcgag gaagcatgca ggcgaaggca cgagttcggg
    14101 ctcaccgtcg cggacttccg cgctctcaac ggatggatcg acagcatcta caacctgccg
    14161 gcgggaggcg cgtactcgaa cggcgttcct cgcggcaggc tcctcaaccc gcgcaacttc
    14221 tttggttcgg agagtagcgg caaggcgctg cgctcgtggc accgagcaga cctgggctac
    14281 acggtagcaa ccggtgtgag tcagctgctc gatcaaagcg cgaacttcta tacgctgacg
    14341 caagcaactg ccacaaatca gccagtaacg agcgctacgg gactgaatgg tcgcttctgt
    14401 ttgtcgttcg atgcgagcaa cgactatctg tcttacccct cgggattcgc agcaacagtc
    14461 gcaggtggca acgacaagcc gtttgcctct tggtctgtgg cgcgattctc gaccgacgtt
    14521 ctcaccacca caaagtattc atatctttgg ggcttcgggt attcgggcgg caatagccca
    14581 tatatagagt tttatggtgg atacacccag acggcctggg tttgctcgaa gtgctcaaac
    14641 accaacgtga acgtatcaac ggccggcact tcgccgggtg ttgcggcaaa tacgaactac
    14701 ttcatcgagt actaccacac gggcacaacc agcgaggtga tagtcaatgg cgtctcgtgt
    14761 acgatgagca atggcggagc gctcgacgct gcgcaagcag acgtgaacac ggctgcgatt
    14821 catgcgctcc cacgatcgac ggttgtttct agcaatgctg cgctcctttg gggtgagcaa
    14881 gtcttcgcgt cgggcaagcc tactgctttg caccggttct tgatgaccac ctacaagaca
    14941 cgtcaatggg gattctaagt catggctgca gcacagacct tcacctggtt ttactctgaa
    15001 gacgaggcgc aagcgcgccg atgccacctg ttgatcaatc gcgctgccgg gtatacggac
    15061 gagcagataa gcgaaggaac gcagatgacc ggcatactcg ggccgggcga acgccccgat
    15121 caacctggat ttggtgtcgc tgttggagcc ggtccggatg gcatcgcggt gcaggacgtg
    15181 ggcgcgatgc tcgtgcagga cgtcaacaac cggctgcagg acgttgcgac tcccgaggag
    15241 tgcgccgagg tccaaagccg gttaacgccc gaggagctca cggaagttcg ctcgtatctg
    15301 caagcggccg cgcctcgcgg tgactcttgg tattatatac ccatgagaga ggagtgacgg
    15361 gggtgcattg gcttgcactg gctctcgcgt tcattgcctg cgcgcatgag cgagagcaac
    15421 ccaaagcccc gccgtgcccc acagacgcag agctggaacg catcaagacc gactgccgcg
    15481 cgcgcgtggt gagagactgc ggggacgtag cagacgagaa ctgtccaacc ttcgttgagt
    15541 gctcgaggcg agtgcgagca tggcaggact gtcgatgagc gctgtggctc aagcgctccg
    15601 catcgcgcgc gctgtcgtca agctcgcccc gggagcgctc gacatcttcg aggagctcgc
    15661 tgaggcgctt tcgtcaaagc ccgctgcgga gcaggagcgt ctagccgggg tgatcgccac
    15721 cgagatccga aggcggagca aagaggcgga cgacgcaatc gagttcatca ggacgggcaa
    15781 gacatgagca tcgacccgac gcagctgaga aacatcctga acagcctcgc aatcgtcttc
    15841 ggctcgctct cgaccgcgct tgtcctaagc gatgcctcgc cagtgctcgc gctagctgcc
    15901 ggcagcatcg gtgcgttcct cagcggctgg gcgaacctga cgaaaccggg agacgtgaag
    15961 gtgccgcgag gatcgatcgt tactgcagta tccgacgagg agagcggcac gcagctgata
    16021 gcagtgacgc ccccagcggc gcagccgaag gctgcggaca ccgagcctcc gccaaccgga
    16081 acctgaaagc acacccgtgc acatcactca cgttgaggcg cgtacgatcg tcacgctcgc
    16141 gttgcgccaa cgctatggac gcgagccgag ccgtcctgtg cgccagctcg cgcaagcagt
    16201 gggctggctc gagaccgggt acgcgaggca ttggaagccg cccggcgacg cctcgaacaa
    16261 ttggggggcc atccaagcgc acgcggggtg gccgatcgag cagaccttcc aatacaccga
    16321 caccaagccc aacgacgacg ggacaagcac agcctacgtg caggcgtttc gcaggtacgg
    16381 gactccactc gaaggggcgc tcgacctcgt caaagtcgtt tactcgggag gtcgcccgct
    16441 cccgccgaac actcggtatc ggcaggagct cgtgcttccc gcggccgagc gcggcgacct
    16501 cttcgcgttc agcgctgggc tttacgacac ggtctattac gagggcttcg gccgcacgcg
    16561 tgcggagcgc atagggcacc acgcgcttgc cgttcgcaac gcctgcgccg ctgcctgccg
    16621 cgagacgcgc gagccgatgc ccgacggaac cgacgtcccg ccggtcatcc gcgtgctgcg
    16681 cgagggagcg agagggcccg ccatctccgt cgtgcagcgg atcgtcggcg ctccggtcga
    16741 cgagagctac gggcccgtta ccgcccttgc cgtcaagcgc tggcagaagg cgcaccgctt
    16801 gaaggacgac ggcgtgtggg ggcccgtttg ctatcaggtt gtggagcgcg agatcacggg
    16861 agaggacctc gacgctctgg acgggtacgc tgcatgaacg acgaggaacg cgaggctacg
    16921 acgccgcggg caccggcgaa cgcgctaccc caccgcgagc cggaggacga tccgatcacg
    16981 gtctcgcacc cgttgatgcg ctatctgctc tcgaagctcg acacgataga gcgtcagctt
    17041 gaggacatga ggagcggtgc acagagcatt gcgtctgccg tcactgcgat acacgagctt
    17101 cagatgagtt caggtcaggt aagggtggac gacgtggtgg tgctgcttgt cgacgacgag
    17161 tggctgctgc gctcgacact cacgcgggtc atcaaggcta gcggcggcac cgtgctttcg
    17221 gcgcctagcg ttgtcgaggc agagaacttg ctcgcgcggg agcacgtgga cgtggcggtc
    17281 gttgatatcc gtctctcgga cggctccaac ggcttcgtcc tggcagagcg cctcgtcgag
    17341 ggcaaagcca acgttggcgt ggttctgatg actggtttgc ttctcgagcc agatcgcgtt
    17401 gccgcggagc gtctgggtat tcgcgtactc gaaaagcccg tctccaatga gacgtttgtg
    17461 caggccgtga aggaagcgta tctcgcgaag caagcacgtg tggaaaggac gagcggtggc
    17521 cagtgacgag tctacccctg gaagcagtgg gcccggtggt cctctctcta gccgcagccg
    17581 tggccgcgct ttggcgtcag ctgatagtgg agcgcaagga ccacgcccag gagattcaag
    17641 cggagcgagc gcggcaggac caagtggaag cggagctccg agcgcagctg aagcagcaaa
    17701 tacgggaaca ggttcagtac acggagttct atctgagatc gctcgaagcg cggcggccca
    17761 aatccttacc gcccgagggg cagaccccgt gattgagttc gaggatgcgt gggacgaaga
    17821 gtcgcccaca aaggtgctcg tcgaaaagct cgaagcgaac aagcgagacc tcgcccggat
    17881 ctatcgagag agcttactgc caccgccgat gccgcccgac tcgagcaagt gatccgatgt
    17941 aagtgagcag gtgaacactc atcaatgggc caatcgtgag gcccatggca gaacccgcca
    18001 tcccgagaag gatgggcggg tttttttttg ctttttgtgc ctctccgtcg tttttcgttt
    18061 acggtttgtc gggctactga gtattagtct actcgccgag cggcactcag ccttagggcc
    18121 ggagacgagg cgtttgagga gtcgcgaagg ggaaacgttt cgatggcatt cagagaggta
    18181 ctttgcgcgt gcggtagttc gtttgtgccg cgctgcgagg cgtgcggaga cgcgctcgac
    18241 gaggggcacc tgctcgcttg caacgagtgc ctcgagttgt tcgttgtcgg ctccgaccgg
    18301 cgcgttcgcc ctgtaacgtt catcgagttc gagttgctct cgccaggaga agtcgaccgt
    18361 tacctgagca gcgctggagc ggacgtcact cagctaaggg ggacggcggt cttcaagcgc
    18421 tacatgaggc tggtcgagca gctgcacgcg gcttgcgtga gttggtgccg ctcgaatccg
    18481 cgagcggctc tgattctcaa cgagccgact gcgtacttcc cggctgatct caaagagatg
    18541 atcccgctgc tcgcgcaagg cgacgcggac acgctcgatt ttctcctagc gatcgacaag
    18601 gcgactaacc acatggcgac actctcccag ttccaggtcg tggtcagtcg cgtcaagcgt
    18661 cctcttacac gaggaggttc agcgtgaagg aagggaccat gatgagcgag aggtcgacga
    18721 cgagtcagga taagttcgaa gaggcgagga tcaacgacaa ggttcgagtg aagatcgcca
    18781 ctccccgcgg cagggaaccg gtctgggccg acgcggtcct gaaggacaag aaccgaggcg
    18841 gcgcgcgctg caggacgagc ctcgggaatg agagcttttt cccgtggggc gacatcatcc
    18901 tgcagcctcg cgagcagcaa cagaagaagg cactcgctac tcttggcgag atcgtcgcca
    18961 ctcacaaacc cgtggtgctg agagctgtgc cgcctacgga gcccgcggca agcgcagcgg
    19021 aagaggctgc agccgcgagc gaccaggagc ggatcgcttc tcctcccgcc cccccggtat
    19081 cgaccgagca atcgacgacg ccggccgagc gcgagaagcg tggtcgtgct cggcttcggc
    19141 atgagctcac cccaatagcg aacgcgatcc gcactcatcg agtccggttg ggcatgagac
    19201 agattgaggt agcggagcgg atcaacacaa gcgcccagag gatcggcgct attgagctcg
    19261 ggaaaatcct ccctacggac gctgacctcg aggggttcgc taagctcgca ggcgtccccg
    19321 cgcaggacct aaaggcgatc cgagatgggg ttccgtttgc aaggccggca gcagcgttcg
    19381 tcgagccggt acgagccgag ccgccaccgc caccgccacc gccaccgcca ccgccaccgc
    19441 caccgccacc gccaccgccc gccgcagctc agacgttcac ggcaaagcag caagagcagt
    19501 tcgcacactt cgccgaggcg ctttgcgacg tcgcgccgat cccggacgac cggggaaagc
    19561 gaacgctctg gttcgcgctc gcgcgaaagc tatttgaggt gagcctatga gtgcgctgca
    19621 ggagtcggcg gagcaggagt cggcggaaca gcgacgggta atcctcgatg cgatggacct
    19681 cgagatcgac cagttctacg tccaggcgca ggccatcggc tttcaccagt tcatcgagtt
    19741 tgccggcttc atgcgagagt tcgtgaagct ctgtcgagag ctcgaggcga ggggcaagga
    19801 cttcaggtgg tgggcagtcg agccgcagcc gtatcacgcg agttacctcg gtgagaagtt
    19861 cgggtgcctg tatgggaaga tgtcgcccga ggcagccctg aagttctgcg aggccgcgtt
    19921 cggtgcgaac gcgagcgagc ttcagaggct cgtggacgag gaagaggaaa gacggtggcg
    19981 ttgggttctc tccgaactcg ctcagtacgt cccgcccggt tacaacccgt tgcgccacga
    20041 cgaccctggg ctctggagcc cggccatgta tcagcacctg atgtggctca ccacggaggg
    20101 ggcaagatcc ggcgagctcc gcggcaaaag cgcgaaggcc atgcgttggg cctgctgggc
    20161 tcaaggtgct ctgtgcgcgc tcgggcacgc ctcgctcgat caatgcaagc gcctgaacat
    20221 gcgtaagacc tgggatgagg cgcggcaacg ccagatcacc aacgcgattt tcgccgacgc
    20281 cgctgagcgc ggtgtcgcaa agaagggaac agagacatgt ccgtaacgac caagcttcga
    20341 gagttgcaaa cgtgcctgca gcaactccac gggctgctgc ctgtgctcga tcaagtgatc
    20401 ccgatgtacg aggcgatcga gaaagcgctt ccgggcgcag cttctctcgc gtctccgcca
    20461 gccaaaccgg ctccagcaat agcggccgca gcggtggctg cgaagcctgc aaagctgccc
    20521 actaaggcag cagcaaagct tgaggagccg aagcccgacg aggcgcccta tcagccgcag
    20581 ggggcttccg gcaaggtttg gtctgtgctg tgcgacgcga ccgactgggt cgggcctcgc
    20641 gagatgctga ggcaccccaa gctgcggaag ttgcccaagt cgtcgatcta tagcgcccta
    20701 aaccgctggt ctgcggaagg gcggatcgag aagcggccca acccgatcca gggaactagc
    20761 gctccgtttc agtaccgtat agtccgcgac tccaaggttg aggggtcaaa gccgcctagc
    20821 gcggctcccg cacagcagcc tgaaaagaag acttccaaga tgtctgcgga cgatcgcttg
    20881 cttgcagcgc tgcagggcac gtggatgacg aagcgcgatt tgcaccgccg agtgagcact
    20941 ctgtctggct caaagctcaa ggtcgcactg gcgcgcctcg tagactcggg cgatgtcgag
    21001 gagcgcaaga gcagcgaggg gatcgtccaa tacaccgcga agcagctgcc cgacagcgcg
    21061 ctcaatctcg gactatcagc aaccgccgcg gaataggccg cgttcggatt gaccgtttga
    21121 ctcgctcacg gcgctgtgtc gccgtgacag aaggagaacc aaggggacag ctacatgcac
    21181 agactgacaa tcacgacttt ggtggtggcg ctcgcatgct cgaacgagcg tcatccgatc
    21241 gagaagaacg caggcgctgg ggggcagcct gggcaagggg gggcgccgtc gtcgatagcg
    21301 acagcggaag gcggggcaat gacagagcag cagcaacagc caacggcggg agcttccgag
    21361 caacccgcgg aggccgcagc tagcggcggc gcagcaggcg ctaccgcgtc tgctggagcg
    21421 ccggcaggcg gagagacggt ggcgagcgct ggcggctctg acggcggggc accgccgacg
    21481 agcatcacga tcagcggtgt agagacagtg cctctcaggc tctcagcagc gggcggagag
    21541 ggcggcgcca gcctgccgtc gttcactacg ccgggtgctg gcctcgtctt cgtcgcggcc
    21601 gagggcgagg ccgtcacggt ttacgggagt ggtcgagagg tagctcggtg ggacgagctg
    21661 accgagcccg cgtggttcgc ctcggacgcc gcgcagacgt acgagctcga cgcagggctg
    21721 aggccagcgg tgagcttcta cgccgtcgtc gatgagggag agccggacgc gatcggctct
    21781 ccctctcgca cggacatggg ggcaacgctt gacgcggcgc tcatgttctc gggcttccgc
    21841 gatggcctcg ctccgacgga cgagcgagtg agcgattggt acgtcttcga cgttcccaac
    21901 ggatcgctgc acgcgacgct ccgtccggcc gccaacatgc gggcggcgct gactctctac
    21961 actccgggtg gcgaggaggt gccgggcaag aacgctttcc acggcctgcc gggaacggag
    22021 ctcacgcttg tcgcacgacg gcttgagcct ggctcttacg ttctgcaggt tcacaatttc
    22081 ggagacttgc cgcagccgag cgggatcggc gcggtgccgg tcaacgcggt gcagccttac
    22141 gagctgcaga tcactgaatg aattaaccag agggagtgtc tccgtgctcg gcttccccgc
    22201 ccttctaccg ggcccggaga tgctccctcg acctattaga ggggtctgat gtccttgaaa
    22261 cgaaaagttc tccggaacgc gcaggagcag cacacggccg cccgagaaaa gtacgaagcg
    22321 gacactgcga agttcgtggc cgcgatgaag gcctggctgc tgatgcccga gaacgctggc
    22381 aaaaagcctg agtttcgcct gcccccccga ggggtcgaat tcttcggcgc gctgaatccc
    22441 gatcttctcg cgctcaacga ggacgcgcgc gacctgctct cctacgtgga tcgcgagacg
    22501 gaccaccagg cgacgctcaa catggctatt ggagtcatcg agagcatcac cggccaagaa
    22561 cggcctgagc ttgaccagga ggcggagaag ctagtggagc tctatgctcg catgctcttg
    22621 agctgcgaat tcggaggcaa ggacaagagc gtttgtccgc attgcagaac tgtgtttgat
    22681 gcacgtgccg cgttagtagg cgaagacccc gacgtcagac caatcgaggg ctcgtacaac
    22741 gtctgtggca gctgcggtga gctgagccta ttcgacgcag cgggcgatct gagaaggctc
    22801 accgcagacg agtgggcgag cgtgccagac ggcgtttccg agatggcccg attggtgaag
    22861 acgcgtgtgg cgcgtaatcg cagtcaggac cagtgaaacc ggggcggagg ataccgtgat
    22921 cacgcattca acgcagagca tcttcgcttc ctcagcagat acgctcgtca acccggtcaa
    22981 ttgcgccggt accatgggcg cggggttggc tctcgagttc agcaagcgct tcccgcggtc
    23041 gatgctggcg cgctacatcc gagcgaccaa aagcggcgag ctcgcgaagg ctggcgactt
    23101 tgctctctgg gagaacccac gagggaagag cgtgctgctg ttcgcgacga aagggcactg
    23161 gcgagatcga tcgcgcatcg tcgatataca cctcggcctg cagcgtctcg tcgaacaaca
    23221 tcgagcttgc tggagtgggc gcgtgaagag catcgctttc ccgcggctcg gctgcggtct
    23281 cggcggcttg tcgtggaccg tcgtgcagcc agttatggag tcgacgctca acgcgctcga
    23341 gggagttgcg gtttctatcc accaccatcc ggagaacgcc ccatgagcgt agcggaggca
    23401 ctgtttcttt cgcgcccccc ttgggctccg ttccatgacg gagtgctctg gtgggcgatc
    23461 cgtggccgct cgtgcatggt gagcgtggag cgcacgggtg cgcgtgaccg cggagactat
    23521 gtcgccaaga ttcatgcgcg cgggatgctc gcgcttgaga tcgacgcgca cgacgggtgg
    23581 ccgcgttact acttcgatgc tcagcgatgc gcgcttgaga ttgaggcgtg gctcaggaga
    23641 cgggggcagt gggtgggcga aggcgaggag cctcccccgt ggggatactt tggggtcgaa
    23701 cagccatgag aacgcgcacc atcatgaggg cgagcgcagg gagcgccttc cggctctcag
    23761 ggggagccct cgcgcgctgc gttgtggagg cgtggatcgt tcaatgtcca aaagctcacc
    23821 cgtgctggtc tcggtggttg gtctcggtga tccacctacg acagctcgac ggggcaccgc
    23881 ctccgcatcg cgagttccca gaagcgagcc acgagttcgc gatcttcgcg atcgatccct
    23941 catgctacga gtcgatcggc tcgggagaga tgaagctcgt gcttctgcag cctccggact
    24001 gcattgagca gttcacggtc cagagcgacg acgacgcggg actcctgctc cgcgaggccg
    24061 tcagcgcgat gacgcacggt tgcatcagcc ccgacatcga cgcgcgagag cagtggcaag
    24121 gcttcgtccg aatgatctcg ctcaccgtcg acaagctgcg caccctgccg gaaaattgcg
    24181 gctgctagcg tcgcgtaaat tactacttgc caagcgccgc ccgatcgcgt ttccatggac
    24241 ctcgcccggg cacctccggc ctcggactcg tgccgaggtc ggccccattg aagctggacc
    24301 ttgagcgtcg ctctgacgct caacctggcc ctaagttccg gcccccttcc gggggtcggc
    24361 cccattgaag ctggaccttg ggcgtcgctc tgacggacaa acgatgtccc gtttccgctc
    24421 cccacctctc gcgaggtgga ggggcggccc cattgatgaa cactccgttg gaagcccttg
    24481 aatgggcact cgcgaatctg cgcttgagcg cggaggatcg gcagaaggtc tccgccaagc
    24541 tcgcatcgct ccggcctgct cccgctaagc agccgcaacg agtcactggg cgagaggctc
    24601 cacagaggcc gtgcccatgg cgggtgtcgc tcgttgccgt gtgcaaatac ctggagatcg
    24661 tcggcaagcc ccgggtgacg cacgggcccg aattcgacga ggcggagcgc gcccttgaag
    24721 ccatcgcgcg ctcggtggtc gaaaagggat cgcccggtca tgagctgtct gggggccggc
    24781 attggttcag aggccctcgc ccgctccggc tgcggcttgt tgtggactat aagaggcctg
    24841 accgagagct gccggcttta atcgacgtgc tacccgactc gccctcgcgc agtcgcccct
    24901 actccaagtc cagtcggtga gccggtggag caagcaccca agtgcccgcg gtgcctccgc
    24961 ctgcaagaga ggcagcccga gatgactgtc ggttggtgct cgacgtgcga gcggtatcag
    25021 tacctgtacc acgttcatcg gattcttgga cgctcagttc cgacacctcg gctgcgtcaa
    25081 ctgcggttcg ggcggagcgt ggtgtcgcga cttcaagatc gatccgagcg ccggcaactg
    25141 cgcaaggctc gctcaccagc acatgcccgg agagtgcccg ggcgacgtgc cgctgctcgt
    25201 aaggcgctat cccaagcgat gaggtcaggg cctcatgcgg tagcagacgg cgagctcgaa
    25261 aaggaactcc ggggtcagcc aggagtcggc accgagctcg attcgcacgt cgccaagctt
    25321 ctcgaagagc ctcggcgtga ggtgaggata cttgcgcagt gcctcatcga cggcctcagc
    25381 gaagccggga tacgagcgga ccttcggagg cgcgctcggc tcgcccagca gcagctcgcg
    25441 cggatcgtcg ttcaggtact tcgcgacgtc gagcaccatc ccgaggcttc caccgtatcc
    25501 gttggcggcg cggcagattg cttgcgagct atagccaaga gtcctcgcga gctccgattg
    25561 attgccatcg aagcgcggca gtagctcctc gacgagcttg cgccgaagcc gctcggagat
    25621 atcgggagga aggcgcgcgc cgttgtgctt cttcattctg taaagcgcgg gcgattctgg
    25681 cggacgtgcg aggttttggc aagaaaagcg gcgaggggag ttgttcccgg cggtgcaacc
    25741 cttaaggccc tatttggtcc gaaaaagaaa gtgccgattg tcgctggcgc gcgcggatat
    25801 cagagtcaat gtctgcgtcg tggcaaaaac aaccaaatac caagacctca ttctttcagc
    25861 actcgggaag gttccttccg ctctttgcga cgaaaacgaa ttcatgctgt tcgctcgggc
    25921 ccaggagctc gatgacctgg tcgcagacca ggtcgaggcg ctccgctacc tcgcggagca
    25981 agtggtgcgc gacgcgagtc taactgagca gggattcgtc gacgggaacg cgctttccca
    26041 cagcagcacc cgtgcgatta cagagcgccg agtagcgatc gaggagaggg caaaggccct
    26101 caggttgttg gttcaggttg taactgggaa gaaagaaata aggagctgag tcgctggcgc
    26161 gcgcggatat cagagtcatg gtgttgctcg tgaacagcaa aacgaaacaa gctaggaagc
    26221 cggtcgaaac ccttatcgca acccgcaagg caaacgttgc tgcgctaacc gagcagctcc
    26281 gggcgctcac tgaacgcctg ccggcgaacg gcgactacgg agtcgcaggg tcactcggca
    26341 gaattgaaga agtactcacg gagctcctct cgggatgggc gcccacgcta tcgcgagacg
    26401 aaatgacgat ctggttcccg ctcgaggcga agcgcgatga ggccgccaaa gcggcgaagg
    26461 gcgagcgggt gacgatcacg acgaagaaga gcaaccgcgg gttcggcctc accgtcgagc
    26521 cgaacgaggg ggacgacata gacgaggcga tcgagatcct ctgccggatc gctgacgatc
    26581 acaacgcgac ccacgaagac tgagcaaccg cggagcgcgc ggatatcaga gcggcaatag
    26641 ggcctcgcag aaatgcgggg cttgggctcg tggaaaggaa ggccacgatg tcccgcacca
    26701 aaaaagcacc gcttgtttac accggctacg tgccctgcgc ctgccgcgac tgcttcgaga
    26761 tcgcgatcgg gttgccggga gaggcgctct gcagcagctg cgaggaggcc ggctgcgagt
    26821 gcgatggcgg tgagtgccaa tccgagggcg cctatagcga actcgagagc ctggaggagg
    26881 aacgatgaga acgactcact tccgcgccct gaacagggcc cacgcggccg ctgcgcgcgc
    26941 ccacctgcgg tttgtagttc ggcgcctgac caaggccggt gtcccaacga aagcgcctct
    27001 gaccgaggcc gactggaagc tcaacgcttt cgccgagcga gccgacgcag aggcgcgggt
    27061 tgctgagctt gagaagctga acccgaattc gaagtacgcg gtcatcgacc tctaactcga
    27121 gagctttgga agaagccctg ccgcctgaag ccgcagggct tgagctcgtg aaaggaagcc
    27181 acatggcctc tcccaaagtc ttgaaccaac tccggtccga tcttgatgcg tgcagcgcga
    27241 tactccgtga gctctcgagc agggctcatc aggtgagtac cgaggcagac ctcgaggcat
    27301 tcgcgcttgc ggccgatcag atgcgtcagt gcgcggagca attgcgcgag ggcgttgcca
    27361 agagctcagc acctcgactc gaggtgctcc ggtaggcgag acgctcggcc cgagctctca
    27421 cggccttgga acgaccgtag gagctcggac cgagcggccc actgcctctc gaatcccgca
    27481 ccgataggaa cactgacaaa tggctattac catcgaagat ctggagcgtc tagtcagcga
    27541 ggcgaaggct gacgacgacg cgcgcaggga gcgcctcgta accctgattg gactctacgc
    27601 tcggctgatt gcgttgcgtg agccgaagcg cttcaaagcg cgcgcaacgg aatactgcga
    27661 cgagccgggc accggcaacg gttcgtcgcc acccgataag tcgtatcgaa accgcacggg
    27721 cccgaggctg atgctgctac tcgagacgca gactgaggac gtgccggata ctggctcgtt
    27781 ctatcactcc tggcaccgcg tcacgcaaga ccccggtctc tacgtcgatg ctgggggcga
    27841 cctgtgggga tctgaggaga gcggcaccgg aagcttctct cagttccccg ctcgtccggg
    27901 tgactgcaac gtgcacgtcc gggtcgattg gagccggcgt accgctagcg acctcacgac
    27961 ggctgatctc gaagcagctg aggcgaagct ccgcaacatt gctgcgacgg tcctcaaatg
    28021 agcgcgaagg agaaatcgct cgcgcctcca gcggccgcag ctgcgcctga cgcgcccaag
    28081 cctgccaagg ccaaacgatc gaagggcgga aagtcgtctg cggtaacggc ctcggagcgc
    28141 gctcagcaac gcatcacgga gctgctcgct gcggtccagg ctcgggccga gcagatcatg
    28201 caggcgcaac ggcagaagcc ggatcgttgg cagcatttcg cgagcctcgc tgagctcgag
    28261 gatcggctgc gacgggtgct cgattgacga ccgaagctcg cggcaggaga tttccctgcc
    28321 gcgagctcac accccaccgg agcccctccc ttgatgagtc aagaccaacc gaagaaaaag
    28381 aagtcgggcc caagcgggcc gagcggcgcc caccaggccg cgcggaggcc ggtccagggc
    28441 agcgaggccc agtgggccgc ctgggacgcc aaagcggccc gtgcgggcct ttcgtgggcc
    28501 gagtgggtac gccagctgct cgacgaggca ccgggcgctc ccgtccgcag ttggcccagc
    28561 tccgaggggc cgtatccggg ccggcgcttg gtctcggcct ccgaccaaca gctccgggcc
    28621 tggacgcgca aggcgagcgc tgcggccctg tcctgggcgg agtgggtgcg tcgcgttcaa
    28681 gacgcagcct gaaaataacg agagccccac gctcggctct ctgacggggg gtatcagagc
    28741 acgtgccgtg cggggggccc tctggcccga ggcgtaaact actcgacagc tcgctgtcaa
    28801 tggtacgaga ggagtggctt ccaaacgcag tcgtgcacga actctgtgaa cagcttgaag
    28861 tcgtgctcat atcgaccgct cgcgtcatcg accgcgagct gtatcggagt gcctcgacga
    28921 cacatgttcg ttccgcgcag ccgatcgaac tgctcgagga gcttgcggtc ctccaggcag
    28981 cgcgcgaggc actcccagaa gtagcggtct actcgcgcgt ccagttgacc tgcctttcgc
    29041 gctgtggttt ttgcgcggga gtgaagcagc gcaaagggag gccgccacca ccggcggagg
    29101 agctcgcgga gcagatcgca cgaagggttc gttggctgcg cgagatgaag ggcctgaagg
    29161 tcaaagagat cgagcagcga gcaggactca gccactcagc agtctcgcgt gtcgagcgcg
    29221 gtttgcgagg cgcgctcttg ccgctcgata cggtggtgcg gctcgcgtgg gcactcgagg
    29281 ttgacctggc ggttctcata ttcggaaacc ccgccggtga agcgcctgct gacgggagct
    29341 ggccagaaag cgtgtggctg cctcccgagc ttccagatcc aaagcgaggc cgccccgcaa
    29401 aggagcagcg cagaccgccg gtttctgcgc agcagccgat gcgtgaaaga ggcggcctcg
    29461 ccaagcgatc aaagtaggcc ttctgcgctg cggaagtcag gcagcggctc gtggtcaatc
    29521 ggagaccaga ggtgcaggca atacgggtgc acgttgacgt gcttcgcctc gggcgggaac
    29581 acctggatgg cagtgcgtcg acgtccgatg aacagagttt tgacgcgcgt ctggtcttcg
    29641 taggacggga ttcgatccgg gcggctgagg gagacgtgga gccagagcgc cgcgccgtgt
    29701 tgctcagcct cgacaatgac ccggaggccg tcattgcaac tgaaggtgct ggcgttttcg
    29761 tatcgctcga tgcagaacca cccggcgggg agcacgcgcg gactatgaat ctcgagaatg
    29821 cgggcgatcc gatcgcgctc gcccggcgac atgtctagga ccgatttctt cattcctcaa
    29881 ctccaagcag gatggccacc gcgcgcagca cgcgcacacg gctatcgttg ttcggaagcg
    29941 tcgagagctt ctcgcgtacc cgcgcgtaag cggtcaggcc ttcccaagag aggtctatct
    30001 cacccagcgc tcttgtcact atcccgcgga gctcttcgac gggcgcttcc gaccctcgtt
    30061 cgatcagccc cgcgagtgcg ccgagcactt cgatgagtgc gagttcgacg cggtcaaccc
    30121 gagcttttgc gtgcatgctt cctcccgtgt gctgagcagt agtcgatgtt cggcgcgtac
    30181 gaatacgcgc agcgacggca gaccgggcga tcacaggtca cttggcggcc gcgtcgagta
    30241 accgggtaat cgcagaggag ctcgggccga gtggtgcacc cgggcgttga gcattgcctg
    30301 gagccgctcc agacaatgcc cacggtcttg cccgagacca caacctcacg tgctcttctt
    30361 tgcacgcttc gcctccctga acttcgccac tgcttggagc agcgaaaggt ccgcagcaca
    30421 catgcaatag ggccgctggt ccgcccattg gcacgcggca gtgactatcg cctgcacctc
    30481 tggggcgagc tccggcgtag cctcgtcgac gacgggcggc gcattgattg gcagcccgag
    30541 atctctgaac gtactcatag cgcggcttcc tgcagcgggg gctcgtcgtc gtcgatcggt
    30601 ggctcgggct cctgctcgtc ttcgcccagc tgatagcctt cgagggcttt cttacgctct
    30661 ccgtatgcct cccgcagcag cttcagctca gcaggcgaga ccttgagccc cttcagctcc
    30721 ggcaccagct gctccagcgc ggctggtgtg ggcgcgtttg caatcctgct cgccattgcc
    30781 tcgaaggcgc cactgcgagc gcctgaggcc cagctggcga gcgcttgccc aatgtcctcc
    30841 gacagcgggg cggagcgcgc gaagaggttt ctgaactgcg ccgggagctt catgatcgcg
    30901 cgctctcccg gcatgtccgt cttccaggtc ggcacgccat ccgagcccgg gtaaagcagc
    30961 atgttcaggg tcatctcgta gatgtagtcg tcgcccgaga tgggcatcca cccgagcggg
    31021 atcggctcct tctcgccctt ctcgagtttg atcttctcct tcgcgcggaa gcagaagatg
    31081 aagtgcatgt tccccatgcg cgtcaggatc gcgttcttca gccgctgccg ttcctgcttc
    31141 ggcttgctcc aagccagaag cttgatcttc tccgccttct tgtagtcgtc gccagccatg
    31201 cggcgcacct cggcctcgtg catctcgagg acgccaccga tgccctcgtg ctcgtggctc
    31261 atgctgtcgc agatgacgat gctcgccttg cggtgttggc agtactcgaa gatcgagagg
    31321 tagtccgccg ggctatacgg agcctcgaaa ggggtgtgcc ggaaggtgaa atcgtccgcg
    31381 tattgctccg ctcggcccga ctccgtatct gtcaggaaga tctctcctcc gacaacgcgc
    31441 tgcatgcccg tcgcgagccg taacgcggag taggtcttcc cactcccgga cggtccgacg
    31501 atcccgatga gtagcggcgt gcggccgcgc gttgctttgc gatcctcaaa tttgcgagct
    31561 cccatatccc tcttcttcct ctagagcgac cgcgtattgc ggcgcttcca agcgcaagat
    31621 ctcgtccgtg tagctgggcc agctgtcctc gcgcaggcag cgagcccaga ggtcaacggc
    31681 gcggcgccag cggcgctcac cgagccaatc gaatgagccg tcgagcgcat acggagctac
    31741 agcgttgggc ccgtcgagct cggcgaagag ccacaggaac tcttcgcggc cggagtaacg
    31801 cgggtaaagc ttcgcgaacg cactgcggta ggcagcggcc tgaatatcgt agccgtactc
    31861 gtatgcgtgt gactggcacg ctctggggtg cgccgatcgc gccgtcttca ggtcgaggat
    31921 ccgcgctcgc ccgtccttca ggatgaggtg gtcaatccgc cctcggcact ggatgatcgt
    31981 cccgtcgctc gcttgttcgg tccagaagag agagacctcg ctgcggcccg tgaagcggac
    32041 gccatagcga gcgaacctct tacggatgac gtcgaccgaa gccgtgatct cagcgagcgt
    32101 tgcgggcagc acggcgcact tgccctgctc acgagcggcg gctcgagctt cctgcgctgc
    32161 cttggtccgc caatcctcgg cgtccaccac caccagctct tcctgcgcag tgcccagcag
    32221 cacagcgtgc gcgactgagc ctgcgtccat gctcgcactt ggctcgtacg gacggccgcc
    32281 aaggcgaggg tgagcgtgcc atgcgtggag cggtgatcgc gagacgagta tgtttgccac
    32341 actcgacgag agggaaggca cttcacatgg gtcggagtgg tagacggccg cgggtaaacc
    32401 gaacacgacc gcgttcggtt taggcttgtt ggtgccgcgc tcgcccatag cgaagcacct
    32461 cccgtccggc tatctgctgc gccgttcgcg cgcgccggca ctcgtattcc tcgagcgttt
    32521 caccgggcaa ctgaggcgtg cggcgacgag ctcttcgcga ggctctcgcc gctacatctc
    32581 ggagagcgga ggatccgccc agataccacg cgataccgcc gacgaaaacg aactcggcga
    32641 acgctagtgc agcaacgacg accagcgcca cggccacctc ctgaattcgt gagtacgtta
    32701 ctacccgagg cgtagcttac gcacaagctt ctgctcgcga gtatgtcact acacggaatt
    32761 cgacatgcta tcggggcggg cgaccgggct tgcggacctt agaaggcctc ggatcatcga
    32821 acaggtcgcg cactcgatac tccagagcgt tggcgatttt cgccagcgag cggatcgtaa
    32881 agttgcggcc gcgctcgtag ctcgagatct ctttcgtgag gttctccggt gctttacgtg
    32941 agcctggttt taggcgcttc cctagctgca gctgtgtcag tccgcgcttg cgcctgagct
    33001 ccgcgattcg cagacccact cgtttcatca cttcctcggg gacttcgggt gccatgcaca
    33061 cgcgacggtg ggcgcgagag agccctgaga atatccccta tgaaccgggg gggtttgcca
    33121 cgcgatgatt tactctccgg atcgtacaaa tttttgtcgt tcggagcggc ttagacacgt
    33181 tcggtcgtgt aaggtccggg cgtcgcggaa aaccgacgcc ccgtggcaac actcgccgca
    33241 caagcggggc tccctaggga ggaaacgata aaaatgctcc gcaaacgcat caaatgcgtg
    33301 ccgctcaaat cattggggga gaagcgcgtc ctcgtgctcg aaacgagcga tagaggcgtg
    33361 cgtgcgctcg cagacgcacc tgctgagatc gtccctcaag cgttggcttt tgttgagtct
    33421 ctggaaaaga agagccgcaa agcaaccagg acgcggctcg aagcggacgc ccccccgagt
    33481 tcggcggtct tacccgcttc gcctccggcg aggcgccgca gggcgacacg tcgctagcag
    33541 gcgcgtctct tccgtacgca tccggtcata ccaatacttc ggacctgggt ctttgcctgg
    33601 accgggatcg gcaaggacct cctcaattgc gccttcgatg acgtcggtaa gacgcgctgc
    33661 gagtgccgca acggcgcgtc ctggatatcg agagtcggac tgcactcgat acactgcgcc
    33721 ttgctgattt ggcgcaccgt aagcgagcca atcaagtgag acgttggcgg ctttcgccag
    33781 cgaagcccac gtgcggaatt cgccaatcat cggctcacgt gagcgcttca acgcacgcat
    33841 tatttggctg acgtgagttg cttctcgcag cccggcccga gcgctccact cgcgagcgct
    33901 ccaattatgc gtgttcatca cttcctgcat acgctcgtat aggctcatga gtacgtaata
    33961 gtctgctcgc ttggagctcg ggtgtgtttg ttttcttgtg agcgttgacc tactcgagta
    34021 gaccactact caacatggac acgcttgctg agcgcgtgag gtgggcccgg gaagctgctg
    34081 gcctgagccg tcggcagctc tgcgctttgg ctggcctgcg ttcaaaccga catgtcggtt
    34141 tcgtagaaga ggggaaaaac gtgtggccag ccacagttat ggccctgtct aaggcgttcg
    34201 gggtgcgagt cggatggcta gtagctggcg aggggaccca gccgaccgaa cgcgcaatcc
    34261 gagcgcatgt ggcgaaactt cggaaacaac aacttggtgg tggttcggct gagccgacag
    34321 ttttcaaggc agccgaacct actcttcaga ggaaagggag gaaagcatcg tgatacggga
    34381 gtgggtgctc gcagcgctgc tcgcgctgcc tgtgtcgaag tacgatcgcg ccgctgaccc
    34441 aacgttgaag cgtgcgcagc tcgagacaat cgctgatgcg atctcggacg cagcaaccac
    34501 cgcgcggtgg tccggaccgc cgcgcgagct cgccgctttg ctgatcgcaa ccgcgtatca
    34561 tgagagctcg ttcgcgctgc acgtgcacgg cggacagtgc cgcccgcacg agtgcgatgg
    34621 cggccgagct cgaggccttt ggcagcaaca ggagcgttcg acttcgtcgc gcttggcttg
    34681 gcttctgctc gccggtctgg acgctgaggc cacgcgcttg gcggcgcatg aggccgcacg
    34741 agccctgatt cgagcacgcg gcacctgtcg cagcttcgag cgcgagggcg cggactggct
    34801 cccgatgacc ttctccgcgt acgctggacg cggctgtaaa gccgcgtttc ctgggcaatc
    34861 ctcgagggtt aacgctttcc ggagactggt cgagctatgg tgaagcgatc catgcgccac
    34921 gcccgtcttg ccgagcttta ccgcttggtg gcgtccgagc acgagcgcct cgcaaaggag
    34981 tcggatgacg agtacgtcga ccaacggcac tcgcctctcg gccctcgccg gcattgcgag
    35041 ctcgttcgca cgggggagct gccgggctac cgcggaagcc gcaagcgcat tctggtgcgc
    35101 cgcgcagaca ttgactcgta cctggaacgc aatcggatcc tgccgacgga gacgaagagc
    35161 gcgccagagt cgttcgagga agtgctcgcc aattaccagc gagcccactg aaagagagag
    35221 aagaaaggga cagaaacgat gggacgaaaa cggaaaggca cagcagtaag gaagcgcaac
    35281 acttggtacg cgcagctcac cgtcgacttc ctcgatggag agtcgggcct caaagcattt
    35341 cggctcgaca acgtgcgaac cgaggccgag gcaaatgcgg cagtcgcgcg cctccaaaag
    35401 gcagtccagg gtaagaggtt cccgcgcaaa ggcggacagc taggtatcgc ggtgaccttc
    35461 gatgtctacc tagagcgatg gctcgaggct cgcctccaac gcggtcgtcg caccgtgcac
    35521 aaggaccgcc agcggctaac ggattacgtc ctgccgctcc tcaaggggaa gccgatgaag
    35581 ctcatcaccg ccaacgatat ccgaggggtg gtcaacgctc tggacctgcg cgtcagcgac
    35641 ccgacggtca agttcggttg gaagacggcc cacctgactt ggcaggcact cacgaagctg
    35701 ttcaaggacg ctacgcacgg caagaacccg gcgctgctgg ttctcgacac caacccctgt
    35761 ctcggtttgt caggtcccga caaaggcacc cagccgacca agcagtggct ctaccctcgt
    35821 gaattcgtgc agctcatttc gtgcgaggag gtgccgcttg actggcgaca ggcgtacgct
    35881 gtagcgacct acttgtacct gcgaattggt gaactccgag cgctgcattg cgaggacgtc
    35941 gacctcgcta gtggcatggt caaggtccat cgctcgacgg gagaggacgg caagacagtg
    36001 cggctcagaa cgaagaccgg agcgggcaga gagttcgccc ttaaccccgc ggtagtgcct
    36061 ttactgcgcc acctgatggc gaaggcgggc ggcagaggcc gcatcttccc gcgcctcgac
    36121 aaggccgcga agcgactgcg cgcgcacctg aaacgggcgg gcgtgcggcg cgaatcgctg
    36181 cacaacagct cgccccacag ccagcagatg acgttccacg atctgcgtgg aaccggcatc
    36241 acttgggcgg cacttcgtgg cgatcggctg atccaaattc gcgatcaggc tgggcacgcg
    36301 gacatcgaaa tgacgaacca ctacatgggc agagcaagct ctgcgggtga cgttggccag
    36361 ccattcccat cgctgtcggt gctctaccca gcaaccggcc ccacggttca tagcacgcgc
    36421 ccaatcacgc gcttcaaagg cgtgattggg gattctgtaa tgtattcggt aagttacccg
    36481 gtggagccga gggggatcga attcccgggg gaggccgaaa aggccgacaa aatcccggtt
    36541 tttgcctctg agctttgccc caactgccgc gagctgaccc gtgcgcgtga tattgcgcgt
    36601 gattcgaaac tcggggaatg tcggccttcg tcagtggccg ccacggctgg cgtcctggcc
    36661 ctgctcgagg cgctggagcg gctcctcggt ccggccgact ctctgtccga gtatcgggta
    36721 cgggcgggcc tgccgccatc gtccgccgag ccgatagcct gcgtcgcgtt caccgcgacc
    36781 tcctgcgttg ccaccccacc ccaggcacag aggggggcct gatggcgacc agcaagccga
    36841 tcgactttgc tgcctaccgc gcaagccgcc tcgcctcgcg tggccctcgt ttgcgcgacg
    36901 tgccgctcgt tctctccgcg caccccggcg ctgtcggcct cgtgatcggg aacaacccgt
    36961 acactttctc gccagcacaa gcgcgcgaca tcgggaacgc gttcgttcgg tttgcggacg
    37021 ttgctgacca agccgggagg cagcgttgaa caccgcgccg agtcgctttg actcggcgcg
    37081 ctgcgagcac ccgcacgagc gactccgaga gttcggcctc gtgcgggaag gcgccgcagt
    37141 gtctgcgctt tggtgcagcc gctgcggcgc gctccgcctc tcacaggcgg aatggttgtt
    37201 acccgaattt gcgctcgtcg accgcctgct cgacgagctc cgcactcgac tgaaacacgc
    37261 aaagccggcc ccatgacagc gcgaatcaca gttcccgcaa cacgggagat ctctcttccg
    37321 ttgccgttct ccgcgcagat gctgcgcgca gttcgggccc accaaaagac tcaaacgcgc
    37381 cgagttgtgc gcggtcaact caagctcgtc agtgggcgca cgctcttcga atcggcctcg
    37441 ccctcgtggg aggcagacga cgaagaagtg ctcgcgctct ggccacagaa gctcacgtgc
    37501 ccttatcccg tgggcgcgac gctctgggtc aaggagggcg ttgaaaagcg caacggccgc
    37561 gccgtttact cggcagacgg tgcgcccgta aagcagctca aaacgtggcg gtggaagcgc
    37621 gacttcctgc cggccatgtt ctgccctcgc gagctctcgc ggatccacct cgaggtgacc
    37681 ggacgacgca ttgagcgcct cacaaacatc accgaggcgg acgctttggc ggagggcatc
    37741 gtcccgagtc ctggctaccc cgcggtgccc gcgtacgcgc agctgtggga ccagctgaac
    37801 gctgatcgcg ggtacagctg gcacgtgaac ccctgggttt gggtcgtcca cttcaagctg
    37861 atcgaagcgc ggagggcggc atgaccacgg tgctctcgcg caaaaagtgt ctcagctgcg
    37921 gcgctgagat cgtttgggtc gaaatgcagt ccgggcggcg catgccgctc gatgcgaagc
    37981 cgcaaaagtt gatcgtgatc gaggccgaca agggcgaggt caagctctgc tttacgtcgc
    38041 acttcgctac gtgcccggac gcagcagagc accggaggaa gccatgacgc agcagcaaaa
    38101 gccgcccgag gagtcggcaa ccgtcgttca acatccgacc gcgctgcggc cgaagaccga
    38161 ccttcccttc gagctcatcg tgaaccgtgg cgggccggtc tgctaccacg atcggaggat
    38221 cgttgacagc acgctgaggg aggtccggtg cgctgcctgc aaggcgtcgc ttgacccgat
    38281 agcggtgctg acgagcatcg cgcaggacca tgagaggtac tccagagcgc tcaagaacgc
    38341 gcgcacggag tgcaacgcgg ccgaggctcg gatccaactc ctgaagaggc tcgaggctaa
    38401 cgcgagagcg cgtgctcgtc gcgctggggt aagcgcctcg aagtcgcacc tcgacgccct
    38461 tgttcggttc atgtctgagc acctgcaggg ccacaaggtg gacgagagca cggtagaccg
    38521 cctgctcgac gggaccacgc cgttaacgcg cattgaagcg gccgcgcagg ccctcaagag
    38581 gggccgagct ggagcagcaa tcgagctcgt gcggcgcgca gccgatgacc tcgaagcatc
    38641 gcttgaggtc gcacagcagg ccctaaagca cgtgggaggc cgccgtggat gagtgcagca
    38701 aactggagga cctgatcctc gaacgcgtgc gggattggtt cgcgcgcgcc gtgccgccgc
    38761 tcgcccggca gacgcatacg cacgcgcgcc tcgagatccg gatcggcgac gcgagcgcct
    38821 ccgaggacgg gtcgctttac ctgcagctcg gcgtctccga ggagctcgaa gggccgctgt
    38881 tcgactcgga tagcggcggc gctgttccgg gcgtcacgtt caagtggggg cgagaatgag
    38941 cccgctagag ctcgtcccct gcacgctggt cgaggcgcac gagtacgtgc ggcagcacca
    39001 tcgtcatctg aagcccgcgc aaggcgccct cttcgcggtc gcctgcgcgg ctgatggcgt
    39061 agtgcggggg gtcgctgtag ttgggcggcc gctcgcgcgc atgctgcgcg acggttggac
    39121 cgctgagatt gtgcgttgtg cgacggatgg ggcgcggaac gcttgctcac tgctttacgc
    39181 ggccgcctgg cgagcatgcc gcgcactagg ctaccgcagg ctcgtcacgt acacgctacc
    39241 gagcgaaggc ggagcatcgt tgcgcggtgc cggctggagc ttggtagcgc agacgcgagg
    39301 cgggtcctgg tcgagacgcg cgcgccctcg agtcgatagg catccgactc aggcaaagtt
    39361 ccgttgggag gtcgtggcgc catgatgaag ggcttcagcg tcggcctgct gccgggtacg
    39421 gtcacgccaa cgtcgggcgc tcacgcgggc acgcccatgc tcccggatag cgcgctcgct
    39481 caagccatcg tcagcgcctc tcgcgtcgcg gagagcgccg cgcagctcca cgaggagcag
    39541 gagcaggccg tcaagaggcg gcgccgcatg cccgagcaac ggccgagcga gagcaagcag
    39601 gattatgcta cgccgcgcgc ccttatccgc gctgtcgaac atcgcttcgg cccgatcgcg
    39661 tgggacctcg cggcgcacaa ggacaacgcc gtctgcgatc tttggatagg cgagcagcag
    39721 gactcgctcg gcgtgcgctg ggcttcagag ctgggcgggc tgctctggct caacccgcct
    39781 ttcgatcgca tcgaggactt cgcgcgcaag tgcgcccgtg aggctcgcga gggcgcgcga
    39841 atcctcatgc tcacgccggc aagcgtgggc gcgaattggt tctgggacta ctgcagcccg
    39901 tcgcgcgatg ccctcgtgct cgcgctgacg ccccgcctca ccttcgaggg cgagacggac
    39961 ccgtacccca aagactgcgt cgtaagcgct tacggtttcg gcacggtggg cttcgagcgc
    40021 tggcaatggc ggcaaccccg gagggccctc tgatgtgggt gaaactcgat cccgggttct
    40081 ccgagcaccc caagttggtg ggcctctcgg acgctgcgtt cagggctcac gtccgtgcac
    40141 tttgctggtg tgggcggaac ctgaccgatg gggtgctctc ggcgggcgct ctcaaatcga
    40201 tgggaacttc caaaaagttg gtccagagct tggtcgactc cggattgtgg gaagttcgag
    40261 gcgaactttt cgtcatccac gactatctcg atttcaatcc cagcagagag gaagtcgccg
    40321 aacgccaagc cgaagtctca cgaaaacgag cacttgcagg taggaagggt gccgagagcc
    40381 ggtggagagg aggtagcaaa cgcgatggca aagctgatgg caaaacggat ggcaaaccac
    40441 cagaaccgga tggcaattgc catggcaaaa gcctggcaaa cggatggccc gatctcgatc
    40501 tcgtaagaga agatcttaat acaaaccacc tcgctaaaga cctgacaggt ttcgcgcgcg
    40561 aaaaccccga tccgggcgag ctctcagagt caaaaccagc tgaaaacaag ctggagcctg
    40621 ttcgcgttgt tggcgccgcg ttccgaaaac gcgccgaaag accacaagtc gaccaggagc
    40681 tgccgactcg gtcgcatcca cgcgacgtcg aggtgcccat agatttcgct gaattcgcag
    40741 acgagctcgg gctttcgtcg caagcgtacg cagacgccgt caccgattgg cgcgagaaat
    40801 gcggcccaag cacgcacaag cggctgttcg atgtgctgtg ccggttcatc gaaagccgcg
    40861 ctcgaagacg ggaaaagtcc gtcgttccga gttcgccgag tgcgcagcag cacgtggtcg
    40921 acgacgcgcc gaggctttgg acaccgcctc cggagcctcc gagagaccca aacccaccac
    40981 cgccgaaccc gatcacggcg ctgcgagcga gggcgtcgaa tggctgaaaa actgctgccg
    41041 cgatgcgatc tcgatgcgga ggccgcggtg ctttcggctg cgctgctctc gcaggacgcc
    41101 gcactcgaag tcgcagaaat cctgcgggcg gacgactttt tcggcgacgc caaccggcac
    41161 gtgttcaacg cgatcgtcgc ggttttggcc gagaatggcg cgtgcgacat cgtggcggtg
    41221 caggcccgac tgcgagcgac ggggcggctc gagcaggtcg gcggctcgcc gtacctcgcc
    41281 cagctcgccg atgcgacgcc ggcaatcgcg aacgtcgcgg agcatgcgcg agtcgtgcgc
    41341 aacctcgctc gagttcgacg cgctggtgcg gtcttcgcgc tgcttgcggc cgaggccaag
    41401 gctgcgccga tcgaagactt cgatgcgtgg ctcgagagct gcgaggcgcg agcatgcgcc
    41461 gcaactgccg agcgtggtga cggtccagca actgcctcgg attatcgcga actcgcagcc
    41521 gatgcctacg agcagttttc ggccgcgagc aaggcaggca agccgatgct cggtcgagcc
    41581 atgggcttcc acaacgtcga ccagcatctc ggtggcgtgg agcctggcga cctcgtggtc
    41641 atcgctggcc gcccgggcat gggcaagacc gcgtatgcgc tgcaggtcgc cgagaccatc
    41701 tcgagcgatg cacgcgacaa gggtctcgcg gtgatcttct cgctcgagat gaaccgcaag
    41761 cgtctcatcg tgcggtcaat ctcgagggtt gttggcgttg ccgcgaacag cctgcgcgct
    41821 ggcaaggttg cggactggag cgccgtctca gcgggcgttg caacgctcgg aacactcccg
    41881 gttgttgtcg acgacgaccc gagcctcacc ccgatgcgtt tgcgagcgaa gctgcggcgg
    41941 cacgttgcgg ggctgcgccg aagcttcccc ggctatccgc tggcgtgcgt ggtggtcgac
    42001 tacatccagc tgatgaacgt cgacaacgct cgcaagcagt caacgcgcgc cgaggagatc
    42061 tcgcagatca ctcggtcgct caagcagatc gcgaaggagt tcaactgcac ggtgtttgcg
    42121 ctctcgcagt tccgtcgcct agacccgggc aaggcagcgc cgcgtccgca gctgcacgac
    42181 ctgcgcgagt caggctccat cgagcaggat gccgacgtcg tgctcgcgct acaccgcgag
    42241 gattattacc ggccgcccga gaagcagacc cacgacgggc gcacagaggt gctcgtgctc
    42301 aagggccgca actcgggaga ggcggtgcac ctcatcaagt tccacggccg caccacgtcg
    42361 ttctcggacg acttcgagcc gagaaaacaa ttggatctga tcaacggagc aaatgggaat
    42421 gggcagcact acgacgactc agaacaatac gaagcgcgct acccctgaag tgaagatcga
    42481 gaagttctcg cgcaacctgc gcgtagaact caccgaggcg gagatcaacg agagggcaca
    42541 gagggcggcg cacctgctgc aggagcgaga ccgcaaggac gaggagaaca aggctgcgag
    42601 caagcacgcg aaggcacagg tagccgagat cgaagccgag cttcggcgcg tctcgagcga
    42661 gatccgcgat ggtgcgacct acaaggaggt cgcgtgcgag cgccggcaca actatcggct
    42721 cggcaacatc caggaggtgc gcatggatac caaggatgtg attgcggagc gcgcgatgaa
    42781 cgaccgcgag cgtcagctcg acctcacgct gaatggtagc aacggcgcga gcaacggcag
    42841 caacgggaac ggcgcgagcg ctcacgccga ggaaggtgat gagcaaccca acagcgtgca
    42901 ggtgctcgag gcctcggagg gaggcgcgga aaacgactcc gagtacgaac ccgaacctcc
    42961 gctcgagacg cagaagcccc aacgcgcgcg caggagccgg aagaagccat gagctcgttt
    43021 gaggggtggg cgatcttgga gctcatgggc caccgccggc tcgctgggcg agtgcaggag
    43081 gtcgagcagt tcggcgcaaa gatgtgccgc atcgacgtcc cgggcgcaga ggcggaccaa
    43141 gcgacgcagt tctacaacgg cagcgccgtt tactgcctga cgccgaccac tgaggagacc
    43201 gcacgcaggt tcgctcaggt gaaccgcccg ggcccggtgg ctctctggga gctgctcccg
    43261 ccggtaaagg ccgagcctcg cgacctcgat acgtcggacg acctcagcag caccgacgca
    43321 gacgacgaca ccaacaattg gtgagggaca tggctttcgt ggacgtggtt ctagggattg
    43381 atctgagcct gactggcctc ggtctcgttg ccgttcccgc ggactgggat ctgctctggt
    43441 cccgcgtcca ccgcatctcg ctcggcgtga agctgtgcga cgatgcatca ccacgcgacc
    43501 acgttatgcg gaggcgcgca ctctgcgatg acgtcgtgcg ctgggcctcg cggcggcacg
    43561 tcacccatgc atgggttgaa ggctacccga tcggcaggcg gaacctgttc aacctgcaca
    43621 agcaggccga gcttcgcggc gttctcgagg atcgcttggc cgagctcggc ctttacttga
    43681 accccgtggc ggaggcgaca gcgcggaagc tgctgctcgg caagctcccg ccgacggccc
    43741 gcaaagaggc cgtgttctca gcgctcgact gcgtgggcgc tgggtttacc gatcgcgacc
    43801 aggcagacgc gttcacggtc gcgaactacg gattgaagga actcggagcg ccttgggttt
    43861 cgtatggcgc accagaggca ccgcagaagc gggccagaaa gaagcgagca gcatgatagc
    43921 ggcgtacatg gcgcatccgc tcggtgcagg tgcacagcgg gaactgaaca acgcaaacgc
    43981 tcttcggtgg ttcggttggt tgagccagag cttccacgtc gcggtctccg cgaattggtt
    44041 gctgctcgca agcgtttggc cagaaaccgc tgagtatcgt gcacgagcac tcgaaataga
    44101 tcttgaactc gtcagccgtt gcgatgagat atggcttgtc ggtggccgcg tctcggaggg
    44161 catggcgctg gagcgcgctc atgccgcgcg tctcggtaaa cgtatcgtcg atctcacacc
    44221 gctcggctac gagccgccga tctggagtta ccaggaaaac gcagagttta agcagctcgt
    44281 cgaggagctg ttcgagcggt cgtacgcggc caacgaaaac aacgagaggg agtcaggcac
    44341 atgttgatga agatctgcag agtagccgtc gagctaggcg agagatgcct gcttgacccg
    44401 ttccagctgg acatggtcaa cctgcagctc gaggagcagc gagtaacgcc gtggattgcc
    44461 caatacagcg gcaacttcgt gatctggatg tgcgggtggc tggacggtgc aacaccggag
    44521 gagatcgcaa gtgcgcgcga gcagaacagc tttgtctgaa cgcgagcagt tcttccgagc
    44581 gctcgacgcc cggctcgagg caggagcggt cgcctacgga gatcgatcct ttgaccgccc
    44641 ctcggctgag ctgataggtg agctgcagca agaggccctc gatatagccg gatggggctt
    44701 tatcctgtgg tgccgcttgg cgcgtctcca agcagctgcg gagagagcgg aggctgcgga
    44761 atgaccgaga cagccgaggc ggagcgccgg tatcgagagg acctcgcctt cagagcgctc
    44821 gcagacgcaa tcgcacgaca cgcacattcg gagagctaca cgcggcacga gatcatgctc
    44881 gccgctgcgg ccgcggttct cctcatcgag cagtggagcc atcgcaagcc agagtgatcg
    44941 aggtgcaccc gtatcagtga gactacgacc tgacgtggcc aatctgctgg cccgaaggcg
    45001 aggctctagt cctcgccaac aggagcctga gcgctgcctg ggcttctctg acaacagaga
    45061 agatcacctg atgcaggtcg aagtctcact aatgcgggtg caccccgcaa caacgtgaaa
    45121 gggacagcac gtgaaacgag gcggcacact catggtcgag attatcaaac cgctggacga
    45181 aacgtggagc gacgtgggtg ctcgcatgcg tgcactgcgc aagactctcc cgcttgcttt
    45241 gaacttcgcg ctccgcgatg tgttctcgcg agccatgacg caagtcgagc aggtcgcgaa
    45301 cggcgagcgt gcggatacgc gatggcagga tgacgttcga aagcggctgc gctaccactg
    45361 ggaagcgcag ctgacgcgcc agggtgcttg ggtggcgaaa cggtcaaaag cgaaggcgcc
    45421 gatggtgtcc gatcgcgctc tggctgcgcc gggtgacgtt ctctgcagcg agacgagcga
    45481 ttacatcacc tccaggttct cggggcagca cttcaaggat ctgctggctg gtcgcgctgg
    45541 ggtgccaacc ttcggtggcg ggaagagctt cttttcagag ggacggcatt gcagcatctc
    45601 gggctcgccg gagaaggccg agctgagctt tccgctgtgg ggcaccggaa agcgcgcgac
    45661 tcggttcgcg gtggcgccct gtggcgacgg ccacgctgcc ctttggcgaa ggcttgtcga
    45721 atctgcagac caccgcgtcg agacgatcga cctgatcaag cagtcgaagc agggcgacac
    45781 caggaagcag gcgctcctag agctcgagac gaagcgcgtg ctgaagatgg gccgcgtggg
    45841 catcaaatac aacgagcgga agggtaagtg gttcgcgctg atctcgtgga cggagtatct
    45901 gcctgatctg atcgaaggcg ggctcgccgc tgcgtgcaac ttcggaatca actgcttcgt
    45961 tgctgcgctg gcagaggatg ggtcaacgta cgaggacgtc ggtacggaca tcattgcgac
    46021 gcgcacccgc tatcagcatc gcaggcgcag ccttcagcgc tgcgtacacc accgtggatc
    46081 cggcagccgt ggcaggggtg tgaagaggcg cgaaaaacca ctgactgacc tcggagatgc
    46141 ggaagcgcga tgggtgcggg atcgcattcg gcgagcagct gctgactttg cttcgtggtg
    46201 cgtgagccac ggagtgggcg tggtttacct ggaggatctg acggggctgc gagacagctt
    46261 cgagcgcgca acgtcgggaa cggcgcacga ggaggtgaaa cgatggatcc ataattgggc
    46321 gttctacgag acgcagtgcg cgtttcaacg gcagctcgag gagagcagta tcgtcgttca
    46381 gcgggacaag gcgaggtttg tctctcggcg ctgccctcga aaggagtgcg ggcacaccgc
    46441 ggcggaaaac gtgaagtgcg tgggtggcga ctgggtgccg gtgccagatc acactctgca
    46501 gcggctgcga ggcatggacg gcgagtaccg ggttacgggc ggcaagcttt accagaggca
    46561 gcgaaagcag aactggttcg agtgcgtcaa ctgcgggttc cgtgcagatg gcgacaaagt
    46621 cgcttgcgcg aaccatctgc aggacgcggg cagagagcaa ccgctccaaa aggcgctgag
    46681 tgctgcgcgc atggtcaccc gggtggagac cgaaaccgcg agcggttccg ttctgaaggc
    46741 cagtgtggtg gccgcagaat gaccacctct cgcaaagcgc cttgtaagtg gtcggatcct
    46801 aaggcgggtc tgcgttaccc ctctcggatc cgcggaggaa tatcaggtga tcagagttcg
    46861 aagaagccct cgcgtgggcg gtggatgccc gctctcggat ccgcggagga atatcaggtg
    46921 atcagaggga acgcatgcgg ctccttgggc tcgaatcgaa gtctctcgga tccgcggagg
    46981 aatatcaggt gatcagagtt gcacgcccct gcaggatatc gaggcgcgcg agctctcgga
    47041 tccgcggagg aatatcaggt gatcagaggc tgcagacgct tggcctccga gcgttcttgc
    47101 tctctctcgg atccgcggag gaatatcagg tgatcagagc ggagctcagc gcgtgcgagt
    47161 gcaggcccga gcaactctcg gatccgcgga ggaatatcag gtgatcagag tgtcgctctg
    47221 ctgcggacag ctctgcgacg acgcgaactc tcggatccgc ggaggaatat caggtgatca
    47281 gagaccccga ctacgtcctg cgcagcctcg tgcgctggcg cctctcggat ccgcggagga
    47341 gcatcaggtg atcagagttc gttgaggacg caccgctcca atggaccaac aactctcgga
    47401 tccgcggagg agcatcaggt gatcagagct agctgagccc cgaggacctc aatggcgagc
    47461 cgcacggaac tctcggatcc gcggaggagg attcatcagg tgatcagagc ggaagttgcg
    47521 cgaggaacca atgactgtcc gcggagatca tcacgtgatc aagagtcggc tgtgtgggca
    47581 gcatcaggtg atcagagggc ttgctgttct acagaaaaga gggagaaaaa gcgatgaata
    47641 gggacaagga cttcgaagag tacagagccg ccgtcgcgcg tgtgcgggag ctcataggca
    47701 attggctgaa cggtggtgtc gatgtctttt tggagctgca gaagatcgac aaaaaaggca
    47761 tctggcggat cgcttactcc tctttcggcg actttctgtc tagagagttc ccgaatgcgt
    47821 tggggctcaa tcgctacgaa aacgtgatcc acgcgattgc cgtgtacggc gagaagcggg
    47881 ttcgccaggt cggagtcgag gggtgccacg cgctgctttg tgcggaagtg gtcaagagcg
    47941 aggacaagcg atccgaactc gaggcgagca tagaccgcca cgtgcaggtg acggggacgc
    48001 ctccaccgcc tcgggagatt cggaggatcg tgcgcggtat cctggagacc ccgtcacctc
    48061 tggccaaggc aacgatctcg ctgcgacgtg acagcgcgct cgcccaggaa gtcgttctgc
    48121 tgcgagctca gctgcgtgaa gccaaaaagc ggatccgtga gctcgaggcg cagctccgga
    48181 aggcaacggg gaagcacacg cccgagggga gagcagccgc agaatgacag caccgccgct
    48241 gctgacggcc gttcctcgag gacgcgttct gcgcgaagcc gatctcgatt ggttctgcag
    48301 ccacgggcag gagatcttcg agagctccgc gttcggcgct gtgctggagc gacaggcgac
    48361 gtacggacag gagtgcgcgc catgcgagcg gtgcggtggc agcggcttta ctaaggacga
    48421 cgggacgtgc cgctcgtgcc gtgggctagg gggcaagccg cgcaggcttc gggctgaggc
    48481 cagttcacgg cgcggccggc tgctatgcgg gagcgcccgg tgcgggcggt gccaagggcg
    48541 cacggggctc aagtgcgacc gctgcggcgg ggccggctat gtcgacacgg ctccgatcgg
    48601 ctcgacctcg gagcggcacg aggagcctct cgcgtttact ccggacgagc aggcgctgac
    48661 gaggtacgcg caggtgagtc ggtggctgat ccgcgctggg cagtgggcag cggtgaggct
    48721 gcagacctac tacggcgtgc cggggcaccg gtgggcgggc accaagtggg gccgcgtgtt
    48781 ctcgctcgtg cccttgaccg gagcgggagc gaaaatgctg ggttcgaccg ccaacccgtt
    48841 ctcgctcccg tccgatgccc tcctggcaaa ctacgtcgag cagctcgatc gcatgcggga
    48901 tgctactcag cggatggcgg ctcgtagccg tgtagcggaa gccacacggc aggccgtggg
    48961 cgatttcgcc gaggcgtgct cccggtggaa tgccgcagtg gatgcgtcac gctcgaggaa
    49021 accatgatgg caatcgattc cggccgtcga aaaccggacg cgccgttgtt ctacacgatc
    49081 aaggacgtgc agcaggcgat cggaccctcg tggagtcaga ggcgcgtcag gcgctggctt
    49141 tcacgagccg gtgcactcga gcgtcgctac gggaccgtgg tcaccacccc cgagcgcctc
    49201 gcgagctcgt tcccggaggt ctatcagcgc ttgctgcgga gcgatgagga cgacgatcga
    49261 gacgaccgcg acgatgacga cgaagacgag tgaactcgca gcacgcccgc tctgcgatct
    49321 ttgcggcaag cccgtcgagg cgtttgccga gagctacgac tccttcatgc gacgcgtcgt
    49381 ctttaccgcg cgctgccatg ggcagatcga gcgcgtggtg ttgaccgaga tcgagtactc
    49441 aatcgtcgag cgcgggctct gcttcggccg agccttcgtc gggccgttgc ggcttggacg
    49501 atgagccccg ctgcccgatt ctctttcgac ggcggcgctg ctgagtttgc tgcctccgca
    49561 ggtgctcgcg gttcagcagc tcgtgcagcc agtattcgaa gccgggatcg tggtcgagag
    49621 tcaaaccagc gacgtcggca atcgcggctt gggagaagcc gagcgtggga gccaccgaca
    49681 gcacgctagc cctgctcggg tacgggtcag gcttcgttgg agcgttcgcc cactccggct
    49741 cgttacgtcc gaagcacaac cagtcgagtg agacctgcgc tgcgttggcg agctttaccc
    49801 aggttgcgag ttcaccggtg aagggcttgt cctcacgtcg cagcgttcgc atcagctggc
    49861 tcacgtgact ctcttcccgg cacccagcgg cgaccgacca gtgccgggct gaccacccgt
    49921 ggtgggcgag gacggcctcg aggcgatcgt ggagagtgag cagcggtttg acgttttccg
    49981 acatcccagg atccatgcct gcgcctgggg cccacaaccg gcaatccgcg ccctcagcag
    50041 agtggctcat gcggatacat cgccacaacg aggcggtgcg ggcgctggcc gagtaaagta
    50101 ttgctcatga attaccgcgc gcgcctagcc tttttcggtc tcgtaatcct cgtcgctgcc
    50161 tgcggaggcg agtcggatgg aggcgccgca gtaaacgaga cgggcggctc taccagcacg
    50221 agctcgagtg gctccagctc gacggcaggc acctcggcca cagcgggcgg cacgagcgcc
    50281 accacgacga cgagctccgg gggggcaagc gcggccggca cggacgcagg aggcgcgccg
    50341 caggcgggcg gagcggcccc tgtcgaggct ggggcgagcg gcgagggcgg agagcctgta
    50401 gcaggcgctg ccggtagtac gagcgcaggc ggcgccacca caggcggtgc cagcgccaca
    50461 ggcggcgcca ccacagcggc gggcgggaac acgatcgcca gtggcgggac ggcagcgggt
    50521 ggaaccaccg ctacgtcggg cggcgctgcg gcgggcggca ccacggctgc gggcggcacc
    50581 accgcgcagc cctgcgagtg cactgagggc gcgtgctgcg atggctgcca ctaccgaccg
    50641 cgctcctacg agtgcgcgca cgatcgggtt gccgagagca gctgcacggg cgccaagacc
    50701 accgaggtct gcggcggcac gaccgcgtat gcgacgagcg tctaccgcag gcttgaacaa
    50761 cagttttgct cgggcaagtc cgcagcgtgt gacggtccct cattcaccac gcttcgcatc
    50821 gacagtccgt gcgacctgga cgccgtctgc gtcgacgacg gaacggaggc caagtgccgg
    50881 ctctgctcgg acctgcgctg gcaatgatca ccaagtaggt tgccggcgga ggcgccttag
    50941 cgtaggttcc tactcgccgg gccttgttgg cccgggccgg tggctcctgt ggagccccca
    51001 acgtcctcgc ccggaaaatt catcgctctc tgtctctgcc ccaaagcgct gtccgcttcg
    51061 ggtcagtggc gggccaacga ggcccgggag cttcggcacg tagccgaggc cgcagaaagg
    51121 acgcaatggc cgatcgaatc gaagtaagaa ccatactgcc ctctaaggct gccttgttcg
    51181 caggcgtgac ccagtgcgga acgatcttgg tcgctccgac tgcagagcag atcgcagctc
    51241 tggactccac ggaccgtgag ctcctcggcg aaatcctcga cgcgagacgt gaactcgtag
    51301 tgagctctcc cggatgggag ggcgtgctcg ctcagttgcg ggtcgagcgc catcgcaagg
    51361 cgcaggccga ggcggacctc aatgcgagga aagaaagcga tcggcagtcg ttgaacgaag
    51421 tgcggtctct ggcggagcag cgggccctgt tcaagcccgg cgagttcccg ctcgtgcagc
    51481 gctgctacta cagcctttcc acgtatcgcg atccggagat cgacgagttg cagaagagca
    51541 ccgatcaaac cgtcgatgag tgccggcggg cgtttctctc ggctgccgat gaggagctcg
    51601 ggctctcgcc cagttcccac atcgttggcg aaggcgaggc cgcaacggtc agtgaggttg
    51661 tgcggaacat cgccaacgag ggcaggcgct tcaaggttcc aaccccggcc gcagccgcga
    51721 tccaagcggc tgcgcgggag atccgccaga cagctgagcg cgttgccgcg gaggcgaagg
    51781 ctcgcctagc ggcggagcag agggcggaac tgcgatccat cgtcgcgcag gctggcacgg
    51841 aagaccagct ggagcgattc gacgaggggc tgctgccgga ggctgagctc gcggcagtgg
    51901 tgcggtctaa ggttttcgcg cagatcaagt tcccctcgta cgccaaactc aagcccgctg
    51961 acgtcgctag cgcgcttgag tgcgagccga gcctggtcaa gttctcgacg cgagagtacg
    52021 acggacctct gaccagggag cagttcgctc ggctgaagca gatccgcgcg ctcgccccgg
    52081 aaggggcagt cgtcgatatt cgggagcacg tggcgtacga ctcggagcgc acaggcgccg
    52141 acgatcccga ggtggtccgg ctaggtatcc gcgtaacggc gaaggtcgga gggcagaagt
    52201 tctccgcgga gttcgccatt tcagacgatt tcaagccacc agcgtcgaag gtgcgttaga
    52261 gtagccgacg tccccgcgcg caccatagcg ggggtgaccc gttcctgtcc ggattggcga
    52321 caaacgtccc caccctttgt gggggtaacg aaaggcccgg aagcttagct ctccgggcct
    52381 ttttttcgtc catgctgagg cgaaacgtac tcgccgagct cccgatcgcg gcccgcaaat
    52441 ctgtgctgtt gagtagccat ctactcgtga actcgagtag cctccgcggg atgacggtgt
    52501 tcgcgttgcc agcatgtgag gcgtgccttc ctcacgtaga cgggggagct gatgcgctgc
    52561 tcgaggcttg cagacggtgc ccgcaatgcg ggcccgagct gcagtcggtg ctcgcggagg
    52621 gcgtgagcca ggaggagatc cggttctacg cggacgtcgc gcgggtgtat cgaaagctcg
    52681 tcccgacagt catcgacgag aacgcgcagg tcgcggtcca cgcctgccta gagatggtta
    52741 ccgacctgac cgagcgcgcg acgggaggct ctccggcagc ggtgctcgga gctctgatcg
    52801 gcatgctccg agacgtgcgc gcacgtctgg tgggcgagaa ctaaggggcc gccgagggag
    52861 tggagtgagg gaaagccggt gctgaagtcc gtgctcgtta ccggcacgca ccgcagctca
    52921 ttgcggggcg tcgtctcgcg tcacctcggc gagtacttcg agggcgagac gcaaggcatc
    52981 gtcttcgtgg gcgacgctga tggggtggac catgaggctg tcgagtggtg tctcgcgcac
    53041 ggcttcacac cgtttgtggc ccacgcgctc tgggctcact tccgcaggcg tggccgcctg
    53101 aagtttgcgg gccactaccg taaccaacag ctcgtcgagg tcgccaagac ttacggctgc
    53161 acaacatgtt ttgcggtgcc cgacgctgag tcgcggggca cctgggattg cgccaagagg
    53221 gcgaagcttg ccggcctcga ggtgtggatc tgcacagcga gcacgagggt gaggttctag
    53281 agccatgaca gatcgataca acgggttctc ggtggtcctc gagaaggacg tgcgctcgga
    53341 cgatgcagag cgaatcatcg atgcgattcg atgcatacgc ggcgtgctga gcgtaacgcc
    53401 tcacgtccaa gactgggagg ggcacatggc ggagcagaga gcgcgggagc actaccgcga
    53461 acagctgatc agtgtgcttt ggccggagcg ggcaaaccga tgagcgagac gatcctagaa
    53521 actcaggtgc gcctgctgct cgagaagcag tgcaaagagc aggcgctcaa gcttgccgac
    53581 gggctgttag agggcgtgcc cttcacgctg ctgtggtttg atttggacac gaccgaaggc
    53641 aacgtcgctt tcgtcggctg ccggatctcc ctggccggta caatccgagc attcgagaca
    53701 ttgctcgcct cgtgggaggg aaccgcgacg aggacgcctt tcccgctgcg gccagacgaa
    53761 tgtgaagcgc tcatggctgc cgtgcacgag acgatcgaag cgcccgctgg catcggcttc
    53821 gcgctgctca tagggcgggg cgattgcacc gcttacatcg cgaaggcgga gcgcgaggga
    53881 gtcaaaacgc tgctgagcaa ggagctcctg cccgcttggc gagccgagct tgatcgacgg
    53941 aaggagcatc agcagtgatc ctcttgctgg agctgtctgg agccgtcttc tcgctgatgg
    54001 ggctcgcgct gctgtacgta ggcgcgtatc gcttccggcg ggcgaccgca atactgcgcg
    54061 aggcgaacgc ggtcctcgac gacgcgctcg aacgacagaa gcgcgcgatg gtgatcgcca
    54121 ccgaggtcga caagctgcgg acgatcatcg agcgaacgac cgacagcgct cagctcccgt
    54181 cgtgagggaa aaggccgcgg gcgcgaggtc gcgatagcgg ggaaaaaaaa cgccgaggtc
    54241 cccgcccgac acgcacggca ggtgaggacc tcggctgcgt ctgctttctg aggactaggc
    54301 gagtattgcc gacaccagcc gagcactcgg aacacagggg gcaagggaca gaaagcccgc
    54361 gacgcatctg aaccttcccg cgagaccatt gcgctgtcaa gagggccaaa agaaccagga
    54421 tcgacacaaa cgaccatgct ctgcagcctc tcttatagga gcgcacgaaa ggggaatcgg
    54481 atgcgacgca aaagggtggt gctgccggcg cagatcaagc agtggcgcga acggcagcaa
    54541 ccggaacgcc caaggctcaa ggctagcatc ggtgagcgcg tcatcgccaa gcagatggac
    54601 cgcgtgtcgt cgatgggcga ggcgctatga tctccgtgga cctcaaggac gtcgagaagc
    54661 tcgaacgcga tctgcagctg ttcgcgcgca agtcgctgcc gtacgcggct agcagcgcgg
    54721 ctaacaacat ggcgttcacc gctcgcaaga cctgggtgag cgagatcgag agcacgatgg
    54781 tgctccggaa ccgttacacg ctgaactctc tccgcgtaga gaaagcctcg cctcgaggga
    54841 cggtcgaatc gattcaggcc gtgctcgggt cgctagcacc ctacatggcg gagcaggagt
    54901 ctggtgcagc tcaccagatc gggaaccgca cggccatccc cacgagcgct gcggcgggtc
    54961 agcagggcgc taagccccgc acgaagcccg ttcgtgcacc caataggctg aaggccattg
    55021 cgctcgcccc gcgcagccac aagggcccac gcaagcagcg caatgccatt tcgatgcgcc
    55081 tcgccctcgc gcaggggaag cggttcgtct tcctggagtt ggcgaagggc agaggcctct
    55141 tccgaatcac cggggcaaag cgcaaaccga aggtgcagat ggtctgggac ttctcccgag
    55201 gaagcaggcc tatcccgaag aaccccacgc tggggcgcac cgtcagctca atccagggcc
    55261 aaggcctgac cttctaccac gaagccctga tcgaacaggc caagcgtgca cgcgtgctcg
    55321 gctactagca tccgcttcct cacgcgctga catgggcgct ctgatgccga agaaacaggg
    55381 catttcttcg cggaatttga atagcaaaaa ccccgcaaat atgcgtgtag gtactgtgga
    55441 aacccccggt atataccgcg gttttgcatg caaaccgcgg atctcgcgca atgtatgggc
    55501 cctccgaccg ggtaaattcc ggccggcaaa gtaaagcgtt gcgtggcccc tcgcgacaga
    55561 tcagcgcccc gaaaggaact tcttccactc gagcgctgcg tctgtcctcg cggctcgaac
    55621 taaccccgcg ctgcgggcct acgagtcgcg ctgacccaag caccataaca ccaactgcga
    55681 gatagagcag tggcggctcg cctggctcat aaccaggagg tcggtggttc gaatccacct
    55741 ctcgcaaccc ggatacacgc gcgcgcttcc cgtcctcggc aggatgggcg caagtcgcgg
    55801 aggcctagcc agcgcgcgag agcgcgcgtg tggtccgacc aaaaagcgcc cgcatgaaag
    55861 agcgtctcta cacgcgcacc gagtttgcgc gcatcgtcgg cgtcaccaaa caggcgatcg
    55921 gccaagcatg caacggcaag ctcggcgagg cctgcgtcgg caagcggatc aacatcgagc
    55981 acgccgcggc aaaggagtat ctcgccctcc acggtgcaaa ggcgccgaag cacctcgacg
    56041 aggactccgc cccagctcag tccaaggcat ccaaacgggc gcaaacgaaa cctgcgggtc
    56101 agcccactac cgcagctccg agcaaaaagc agacggctgc tccgccccag cagagctccg
    56161 atccggtagg caaaggacct gtccggctga tctatcgtga ggtcaacgac ctcaacgaga
    56221 tcgctgacat gacgctccgg caaatcggcg cggcgttcgg aacggtcacc ggcttccgag
    56281 attggctcga ggctcgccgc aagctcgagg agctgcggga gaaagagctg cgaaacgccg
    56341 aggccgacgg gaaactcatc tcgatcaagc tcgtgaggac gcacgtcttc ggcgcaatcg
    56401 agtcgggaaa ccgccggttg ctgaccgact cgcccaagac gatcacgcgc cggctgtacg
    56461 cagcagcgcg cagcgacgtg ccgatggagg aagcagagca gatggtccgc gagatcatca
    56521 gctcgcagtt gaagccggtg aaggaaaccg cggttcgtgt gctgcgcgac tcgagtgccg
    56581 catgaaacgt gactggtaag caggggaacc gtcctgaaat gactcgcaag accacagcag
    56641 agccgagcgt gccggcctcg aagaagctcc gagctgcgcc gcccatcctg ccgcaatcga
    56701 tcgagccgac tggctccggc gttgccgcgg agtgggttcc cctttccacg cttcgtgctt
    56761 gggcgcgcaa cccacgacgc aacgaagcag caatccccca ggttcgcgat tcgcttatcc
    56821 gcttcggttg gggaagaccg ctcgtcgtcc gttccgcgaa ccgcgagttg atcatcggtc
    56881 acacggcgag cgctgcggcg gcgcagttgg ccgagctatg ggcggacccg aagtacgacg
    56941 agcggtgtga gaacgacgcc tctctgcgaa aggaccgctg gcaccctgag gcgatccgca
    57001 ccgcggagac cggcttcgtt ccggttcggt tcaaggacgt ctccgagagc gacgcccacc
    57061 gcctcgcggt agccgacaac aagctcggcg agattgcgga gtgggatgct ctgggcctcg
    57121 ccgctctcct tcaggagttc ggcgagcagg gcgcagcgga ggcggggttc agcgctgagg
    57181 agttcgccaa gctgctcggc ggcattgagc cggccaaagg cgaagacgac gatccactcg
    57241 agctaccgcc gaagggcaag gcgataagcc gcccgggcga ggtctacgag ctcgggcctc
    57301 accggttgat ctgcggcgac tcgcgcaacc cgaaggtttg ggcgaagctg ctcgggaagg
    57361 agcgcctgca catcgtctgg acggacccgc cctatggcgt ggactacgtc ggcaagacga
    57421 aggaggcact caagatcgag aacgacgcgc tgaccgagga acagctcctc gagctactgc
    57481 gcgcatcgct cggcgcggcc gccgcgatgt gcatgcctgg cgctgcttgg tacgtcgcat
    57541 cacccggcgg tccgctgttt cacaccttcg gtaccgtgct gaaggagctc ggcatttggc
    57601 ggcacacgct gacctgggtg aaagacgtgt tcgtgatggg gcggtgcgac taccactaca
    57661 agcacgagcc gatcttctac gggtgggagc ccaacggtaa acactactgg tgcggggcac
    57721 ggaacttcga cacggtgctc gagttcgagc gtccgaagcg gagcaccgag catccgacga
    57781 tgaagcctcc cgagctcatc cagcactgca ttcgcaatag ctccaagcct ggccaactcg
    57841 tgggcgacgg gttcggtggc tcaggcagca cactgattgc cgcggctcgt gagggtcgca
    57901 aggcaagact cgtcgagttg gacccgcgct actgcgacgt cattcggagg cgttggactc
    57961 gctttgctga gtccggtggc atgacgcccg gagccggcgc actcgcagca gcgtagtctt
    58021 tgatcggggt gccccaagcg atgtgttctg cgcgcgttgc agatccactc gagacccgcg
    58081 cgcagcggga gtggctcgct caacaattcg atgccctcac caccgaggtc atggttgtca
    58141 gcccctccgc gtgggcagag cggaagcgct acctcccgcc gcaggtcacc agcatgccgg
    58201 gctactaccg gttcgaggtg gcgccgtacc tccgggagat tgcggactgc atgggcatcg
    58261 acagccccat ccgcgaagtc agcgtgatga agggcgtcca actcgggttg accgtagggg
    58321 tgctcgagaa cgcgattggc tattacatcg agtacgtaaa gaacgcgccg tgcatgctcg
    58381 tgaccgcaga caacgagctc gcaaagctgc gcgtcgattc ctacatcact ccgatgctcc
    58441 actactcgga gctcgagcac ctgatcaaat catcggacga gaacaacgcc cgcaagacag
    58501 ggaagaccga caaacgaatc gagtgggttg ggggaggctt cctcattccc atcggcgcgc
    58561 agaacgctaa caagctccgg tcggtgtcca tccaggtttc gctgctggac gagatcgatg
    58621 cttggccaga gcgcgtgggg aaggacggcg acccgctccg actcgcaatg gaccgcacgg
    58681 tcgcgtacga ggcgagcagg aagatcctgg cgatctcgac gccgctcatc aaagggcagt
    58741 cgagaatcga tgcgcgctat cttcgaggcg accagcggcg ctacttcgtc tgctgcctca
    58801 agtgtggcca cgcccaaacc ctcgagtggc gtcgcgagaa cgccgagaca ggcgaggtta
    58861 cagggatggt ttgggagacg gagaacggcc gtctcgtgca cgactcggtt cgctatctct
    58921 gcgagaagtg ctctcaccct cacgtcaacg acgacaagac tcggctgctc tctcccgaga
    58981 acggcgcgga gtggcggccc actgccattc caacgaatcc cgaccaccgc agctaccata
    59041 tcagcgctct ctactcgccg gttggcatgc agtcgtgggg cgcgtgcgtc gagcgctacc
    59101 tcgaagccta cgacgttgaa aacagccggc cgcgagacat cgctctctac caggtctggt
    59161 acaacaactg cctcggtaag ccgtaccacg tgcggggcga gcagcttcgc ttcgaggccg
    59221 tgtccgtgca ccgccggcac gaataccggt tcggcgagat acccaatctg tggacgctgg
    59281 gtcactgcgg cgggccggtg ctgctgctga cctgcgctgt agacgtccac gccgactcac
    59341 tagctgtcgc ggtcttcggt tggtgccgag ggcgccgcgc aatcctggtc gactaccacc
    59401 gctttgaagg cgacacgtcg cagctggacg acgccggcac gtgggcgcag ctccgggagc
    59461 tcatcgaaga gcgggagtac gctgccgacg atgggaagcg ctatcgcgtg cagctgaccc
    59521 tgatcgactc gggctatctc gcggacaagg tctatgagtt cgccgctgaa tatcaagtcg
    59581 gcgtccaccc ggtgaagggc cgagatcagc cgacgaagtc tgcgcgtatt cgtgagttct
    59641 cagaattcac gacgcccatg ggcacggtcg cttttcacat cttcgtcgac ctatacaagg
    59701 accgttggag cgcggctcta aggcgtgggt gggaaggcga aggcgtgcag ccgcatgggc
    59761 acttcaacgc cccgagcgac atcaccgacc agcaacttcg cgagctcacg gtcgaggtga
    59821 agcgcgagat catcgagaag gcctcgggca agcgcgtggg cttcgagtgg ttccgtccat
    59881 cgggcgcagc caacgagctc tgggatctcc tggtctacaa caacgccgct ctcgacctga
    59941 tcgcctggga cgtgtgccgc aaccagctcg gttcggagac cgttagctgg caggacttct
    60001 acgaccaggc cgagcaaaac aagctgttct tcaccaccac ctgagggcct ctcatgtcga
    60061 tgctcgaaga ggacgccttc atcgcggcgc gcattgctgc gatcgaagct cggatactcg
    60121 tgtatgaggc ggcagcgctc gcgatcaaca acggcgcttc cagctactcg tttgacgacg
    60181 ggcaaacgcg catgagcgtc acccgcggcg acttgctgcg gctcaacgag acgatcgaaa
    60241 agctagtggc gcaaagagat atgtggctcg cacgcgtgcg cggggcgggc gcttacgtac
    60301 ggccggggtt ctgatgtttg ggctattcaa gcgcaagcgc gcggacgtct acgactcgct
    60361 atttggctcg ggcgccgcgc cgctctctct acctcccgca gggctgcagg ctgaggctga
    60421 ggcgagcatc cccactctcg agctgtccgc gctctcgacc gcgccgctca actatcgagt
    60481 ccacgacggc tcgaagttcg acggcgggta cggagccacc gatctgctct ggaccgacta
    60541 ctggactctg cggcagcgct ccgcccagct gttccagacg aacctttatg cgcgaggcat
    60601 cattcgccgg ctcgtcacga acgagatcaa caccgggctg cacctcgagg ccactcccga
    60661 ggaagccctg ctggggcgag aggaagattc gctcgcggac tgggcggagg acgtggagaa
    60721 ccgcttcgct ctctggtccg aggagccaac cgtctgcgac aaccgcgagc agctgacctt
    60781 cggcgcgctc caagctctag cgaggctcga ggcgctcgtc tgcggagacg tgctcgtggt
    60841 gctccggcaa gaccagcgta caggcctgcc gcgcgtccaa ctcattagcg gcgccgctgt
    60901 gcaaacgcca tggccccagg tgaaccgccc tggcgctcgc atcgtcgagg gcgtcgagct
    60961 cgactccctc gacaggcacg ttgcgtattg gatcgcgcag gaggacggca cgttcaaacg
    61021 cctgccggcg tggggggaga agacggggcg gcgcctggcg tggctcgtct acggaaccga
    61081 gcgccgtctc gacgaggtgc ggggacagcc gctgctcgct ctcgtgctcc aatcgctccg
    61141 agagatcgat cgctaccgcg acagcacgca gcgcaaggcg acgatcaact cgctgcttgc
    61201 cgtatggatc aagaagactt cggacaagcc gagcacgcgc ccgctgaccg gcggtggcgc
    61261 catgcgccga ggtgttgtcg agacccagga ctcgacgggc acagcaccgc gcacgtaccg
    61321 aatcgcggag cacatcccgg gccttgtgct cgacgagctg cagcagggcg aggagccgca
    61381 gccgttctca tcgcacggca cagacgagcg cttcggtacg ttcgaggaag ccatcgtgca
    61441 gggcatcggt tgggccctcg agatcccgcc cgagattctc cggctcacct actcgagcaa
    61501 ctacagcgcc agccaggccg cgatcaacga gttcaaaatg tatttgaaca agacgcggaa
    61561 ccaatggggc aaagcgttct gccaacccat atactgcgac tggctcttat cggctgctct
    61621 gctccgagac attgaagcgc cgggatacct cgaggggtgg cgcaaccctg gccaatacgt
    61681 agtcgtcggt gcttggagtt ccgcggattg gaccgggcac atcaagcctg ctgtcgacca
    61741 atcgaagctc gtcggcggat acaaggagat ggtggcggag ggcttcatca cgcgcgacag
    61801 ggcgagccgt gagctcaccg gcacgaagta ctcgaagaac gttgcgaagc tcgctcgcga
    61861 gaacgcgatg ctcgcggagg cgatcaagcc tctagcgaaa gagccctccg agccagagcc
    61921 tgcgatcgag cctgaggaag agccggaaac gccagtgcgc cgttcacctc aaccggtaca
    61981 gaggaaagcg agttagccga tgtggcttat tcaggatgag gtcgccgccg caatggccca
    62041 ggcgatcaag gcgggagtgt gcccaacggc tgagcagcaa gcggccttcc tcgagagggg
    62101 ctcagctgag ctgctgggcg caggcgcgga ctccctgccg cgcaatatgg ttgtcgcagg
    62161 ggacacagcg gagatccgca tagatggcgt gctcacgccg aagccagata ttatcgcgtt
    62221 cctcctcggc ggagggaaca cgacttacgc gagcattcag gccgcgctag ctcgggctca
    62281 gaccgacgga gccatcaaga gggtagtcct gaatatcgat agtccgggcg ggtcaatggc
    62341 cgggctcttc gagacgcttg ctgcaatcga agctttcacc aaaccgatcc gagtagtggc
    62401 ctcgcgagcc gcatccgctg cttacgcgat tgccgctgcg acgcgcggca ctattgaagc
    62461 cgttagtcca gcatccgagt tcggaagcat tggagtcgct gcgaagctcc cgcttgacga
    62521 ggacaccatc gacctgacga gcaccgatgc tccgaaaaag cgtccggacc cacgcacgcc
    62581 cgaggggcgc gccgtgatcg tcgaatacct ggactccgtc catgagctgt tcgtctccgc
    62641 catcgcgaag ggtcgcggtg tgaagccaga gacggtcaac caaacctatg ggcgcggcgc
    62701 aactctcctc gcgggagagg cgaagcgact cggcatgatc gacaagatcg caaagccacc
    62761 cgctgtgcct gctgcgggtg acgagagacg cgcgcgcgca atgcgcgaga gcagcgaacc
    62821 agaggcaaca acaatggatc tcaacacact caagaccagt caccccgaag tctatgctgc
    62881 tgccttcgct gagggccgag ccgtggcagc taaaccaagg ctcgtcgagc cagagccggc
    62941 ccaagtcgtt ccaattgcac ccgctgctcg cgcaccggga caggagaagc cgatggacct
    63001 gaatgcactg cgtacgcagt atccggagct gtacgcctca gcggttcgcg agggggagct
    63061 caaggagcga gatcgcgtca ccgcacacct caactacggc accgcgtgtg gcgacatcaa
    63121 actagctgtc accgccatca aagccggtga cccaatcacg cagacagcga tctccgaata
    63181 catgactgca ggcatgaacc gcagcgatcg agctgcgcgc caagccgaaa ccgaagccgc
    63241 cgggagtgtc gttgctggcg tcaagccgga ggcggcagac atggcggata cggtcgcgga
    63301 tctcgtcgag cgcgagctcg gaaagaaggc tgtgtaatgg ccaacatcgt caccaccacc
    63361 attcctgcga atggggaaat cattctgcat cgcgaggagt tccgcgacga actgctcgtg
    63421 tttgctggag caggcacagt caaacgcggc actatcctgg cgcgcgtcgg cggcaacctc
    63481 gtgcccttcg tggcgggcgg cgcgggcggc gcggaagtgc ccgtcgctgt gctcgcaact
    63541 aaggacgacg taacggcgac cggcgcgggc aatgtctccg tgcgggcgct cgtggctggt
    63601 accgtgaaca agaaccgcct catcatccac gcggatggga atggcgtgaa catcaccaac
    63661 gctattctcg acaagctgcg gaatgtgtcg atcgttgcag tcgacgttgc cgtgctcggt
    63721 cccattggct gactgagtat ctccgcgaaa ctcgatatca gctcaaaaac ttaagaagga
    63781 aaaggatgct gcaatgaccg acgcaagcac gcggcgcatt attgcgtctt acacgcagga
    63841 gtcgcctgct cccatgttct tgcacggctt cttccgaagc cccccgcaga actaccacag
    63901 cacggagatg gtcgagatcg atgtccagcg cgacagcgat gacgtcgcga tcgtgattca
    63961 ggatatcgcg actggagcgc ggcagaacga actgccgggt tacgtgaaca aggcgttcgc
    64021 tccacccatc tacgacgagg aagtgacgat cagttcgtac aacctcatga agcgcgactt
    64081 tggcgagagc ccgtacgaca accctcaatt ccaagcgaaa gtagtcacgg ccgcgttccg
    64141 agggttcagg cttgcggaga acaagatccg tcgggcgatc gaactccaag cttcgcaggt
    64201 cctgcagacc gggaagctca cgctcacgga cgccgcaggc aacgccctgt tcgctctcga
    64261 ctacggccct cgtgcagcgc acttccccac ggtaggagtt acgtgggggg cggacgggac
    64321 cactggcaat ccgctcggcg acattgcggc cctcgccgag gtgatccgca cgaacgggcg
    64381 gagagagcca aagaccctaa tctttggctc cactgcgctg acgcgatttc tcgcgaacaa
    64441 ggcgctcaag gagcagttcg caaacgtcgg gttgcagcgc ctccaggaag tgaatcctca
    64501 gcccgcctcg cgcggctcgg gtgccacgaa ctatgggcgt gtattcatcg gcggatattt
    64561 ctacgagatc tggtcgtaca acgcgaccta tcgcaacccg cagacgggcc tctcgactcc
    64621 gtacgtcgat ccggacaagg tcatcatgat ctcggacggg gcacggctcg attgcagctt
    64681 cggcgcgatc cctcgagtgg gcccaccgga tccgcgcgtc ggtcagttcc tcccggcccg
    64741 catcacgggt ccggatcaag ggatcgacct atccaccttc gcctggctaa cgccggacgg
    64801 aaaacacctg aaggtgagcg ccggcacacg gccgctctgc attcccactg gcatcgacga
    64861 gttcggctgc ctcgacatga cggcgtgagc cgcagccgag cagccagaga aggaaagccc
    64921 acgaccatgg caacgaatag agaactgatc gaccaagcgg aggccctcgg caagaagctg
    64981 ggagagcccg tccaaaccca gggcttgagc agcagcgcac tccaggacct cgttacggat
    65041 ctgcagaagc gcgtggacca ggctgcgcag cttcgacccc tcgcaaacga gccgcccaaa
    65101 agccgtgagc cgaacaagcc caacgaccag ccgactactg cggagcgcaa cgcaggacag
    65161 caggcggggg agcagccgaa gtctccctac ttggtggcga agggtcgttc cgtcatcggc
    65221 gctcgaggcg tgatcgacga aggcaaggag atcacgcccg aggacgtagg cggtcaggag
    65281 aacctcgacc ggcacgtcgc agccggcaac gtggataagc gctgatgcct ctctggctgc
    65341 aagccgccgc agacgccaag gccatccttg cggacgtggg gggctttgct cgcccgatca
    65401 cgattacgag cccggcaggg ttcaccgtgc ttgtgacggg cacagccaga gatatttcga
    65461 cgacgattga tcccgcgacg ggcgcagtcg taagcgcgcg caccgcatcg atcacgattc
    65521 ccatcgagcc gctttctgcg gcgggtctct ccataccgcg caacgtccct gacaagacga
    65581 gcaagccttg gctagctcag ttttacgacg ctgcgggggt gctccacgtg ttcaagatct
    65641 gcgagtcgca tcctgatctg acactcggaa tcgtcacttg cttgcttgag acttaccggc
    65701 tcgctccgta gggacacatg gatccgcaga tatccgagct gctgctcgaa gcagacggct
    65761 tagtactcgt gcgcgaccag attgcgacca tactcgcagt cgagtctgca gggcagatgg
    65821 cactggccgc ccaggttggt gccgacccga tgccctggta tctgcgtgtc ttcacggagc
    65881 ggacggcacc ttgggagtcg tacctactcg agccctcggg cggcacgagc gatatgggtc
    65941 cgatcgtcaa cgtgagttgg gagagcactc agttcgacct caagcacgga aacgttgtgt
    66001 ccgcgcaaag aggtggaggg ctcttccaca tcgattgtta cgcatacgca gtcagcgctg
    66061 atgacgggct ctccggtcac gtaccagggg acgagcaggc gaagcgcgaa gtgcagcgcg
    66121 tgctcggatt ggtccgcaag atcctgatgg cggcgcacta tacctatctg ggtctgcccg
    66181 gaagggttgc gcgccgctgg gtgactgcag agaacgcgct ctccgcagct gtcgacgatc
    66241 gctcgcttca gcgcgttgcg gctgcgcgcc tttctctgca ggtcgagtac ggcgagcaag
    66301 caccgcaagt tacggggccg ccactgcgag aagtcgtcac cagcttcaag agaagtgaca
    66361 cgggccagct gatcgctgtg cgccgcaaca tctagcgagg gttatcatat gtcaattgac
    66421 gcttcggccg ttgcccgcgt gacggggatt accacgcagt tccaggacat gcgtgcgggc
    66481 agcgtcctgt tcttgccgca acaaatcgct gtgttcgctc aaggcaacag cgatgctctg
    66541 tacagtgatg agaagtggtt ggctcctagc gctggagcta ctggggctcg cttcgggttt
    66601 ggctcaccgg cgcacctcat tctccgcgag ctcatgcctt cgaacggaga cggagtgggc
    66661 acaatcccgg tgcgcgtcta tccgctcacg gctcctgccg gcgcagtagc ggcgcaaggc
    66721 gaactcacgg tatcgggtac cgcgaccaaa ggtgctgcct accgactccg cattggtgga
    66781 gtgctctcgg aggcgttcgt catccccaaa ggcgctgtga acgcggccga cgtatacggg
    66841 cgaatttccg cagcagttcg ggcggtcctg gagatgccag tcaacgtctc gcactcctat
    66901 gggacgccca cagcatcgca ggggccgaat gagggggacg gcactatcgg ttcgctctcg
    66961 gtttccggaa caccatcccc ggggacttgg cggctcgtcc tgacgaacga ggtcgccaac
    67021 ggcggcattt ggtcgttgat cgacccaagc gggacgacgg tcgcgaacga cctcacgatg
    67081 atcgtggggc caggagccac taccgcgttc tcgcgtgcgg gtctcaactt ctcggtgacg
    67141 gatggcgctg tgaacttcgc ttctggcgac gagtttcaaa tcacggtgcc ggcaacgagc
    67201 gcgcgtttca cggcaaaatg gaagggcgcg agcggcaacg cgatcaaggt cgaggtgctg
    67261 accgacgagt acgtaggcgt ggtgtttgcg gtcacgcagc ccaccggagg cctgctcaac
    67321 ccaagcgtga cggcagcgct cgagaaggtc ggtcccgtct gggagtcgat ggcaatcaac
    67381 gcggcgccga tcgaagacac cacgatcctc gaccagttca gggagttcgg cgagggccgg
    67441 tggctgcccg aggtaggtaa gcccatcgtg gtgttcacag gcgtgaccca ccccacggtg
    67501 gagctggcca ctgcagtcag ctcgggccgt cgcgacgacc gagtgaacgc gcagctcgta
    67561 gcacccggct cgcccaacct gcccttcgtg gttgccgctc ggcagctcgc tcgcattgcc
    67621 aagatcgcga acaacgatcc cgcgcgcgat tacggcagcc agcgagcaac ggggctcatc
    67681 cccggacccg atgaggacca gtggggctac ttgcagcgcg accaagcggt gaagctcggc
    67741 agctcgacca tcgagatcaa agacggcgtc gtagctatca gcgatgtggt cacgttctac
    67801 cgccccactg gggaggagcc gcctgcgtat cggttcgtca agaacatcgt gaagctgcag
    67861 acgtgccttt acaacgtctg gctcgctttt aacaccgagg actgggacgg tgctccgctg
    67921 attcccgatg accagccgac ggtaagcggg agtgcgaaga agccgaaaac catgcgtgcc
    67981 ctcgcggcag ccatcgtcga cagcctcgga ctacaggcga tcatctccga tcctgcgaga
    68041 gcgaagaatg gcatcgtcgc gcagatcagc acgcagaacc ccgaccggct cgacctcgag
    68101 attccggttc aagtctccgg caacaccaac gtcaaagaca tccggcttaa gtggggcctg
    68161 tatttcggca ccgcagcggt cgtcggctga aaggattgat aggccatggg cgtagctatc
    68221 ggtggttcta tccaagaggt ctcaatcgac gggcggctct ttgctgt
//
LOCUS       DASTKU010000175         5599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1817835, whole genome shotgun sequence.
ACCESSION   DASTKU010000175 DASTKU010000000
VERSION     DASTKU010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817835"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFQ61_06710"
     CDS             <1..187
                     /locus_tag="VFQ61_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954174.1"
                     /translation="VAEYRQRTVQGFRRVAVLVSTTAGQLQVQRHIDEEKLKTARVFV
                     DWEEALVWLRQTPSRLP"
     gene            complement(208..984)
                     /locus_tag="VFQ61_06715"
     CDS             complement(208..984)
                     /locus_tag="VFQ61_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9954175.1"
                     /translation="MASALTTAFGAEIVRVPLLPLGMLNAHIVFRDQRAIIVDAGLPG
                     TAPRFERALAERRLGWGNVRLIVVTHGHVDHAGDAARMRDLSGAPIVAQRRELPVLQG
                     EEQVTRCPTGWFGRAFLKTRLPFQAFEPFRADVVVDDAPMDLASFGIPGQLLPTPGHT
                     AGSLSVELPGSVALVGDLVASGILLGGIAFTERCQQPPFEDDPAEVADQLERLLETGT
                     ETFLLGHGGPVTGAAVRQHVRRLRQIARMGRSARAQPAKG"
     gene            1048..1887
                     /locus_tag="VFQ61_06720"
     CDS             1048..1887
                     /locus_tag="VFQ61_06720"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9954176.1"
                     /translation="MLEVVPFAAPPDSLLDYAVRLEQCDVLWVGEGEFAIRCEPQRPD
                     DTVKLLLNIEGSAELKQQGRWAVIERGTYSWVIADQPFELVSGAGSRQLQIRWPRAVV
                     GQRHPDLDTTPAVTRGLDHAGERFICGLLKTLAHEGPKLDRGSHEALVATLLEALGLA
                     KRRASQDPLDQRIARARRDIECLLHDPSMGPVQIARRQGVSRRYLDGLFQARCAETLS
                     AVIRRRRLERAAEQLVSEASHSVESIAHRWGFRNASHFSQAFREHFACSPTGYRSRFP
                     HRP"
     gene            complement(1933..3954)
                     /locus_tag="VFQ61_06725"
     CDS             complement(1933..3954)
                     /locus_tag="VFQ61_06725"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET9954177.1"
                     /translation="MNSRVVEPMEGSMVERHRTDIDGLRALAVIPVVLFHAGISRLAG
                     GFAGVDVFFVISGFLITGIIDRELSQGRFSIAKFYERRVRRIFPALFTMFAVVTPLAA
                     LLLLPEHFRDYCGSLIAATLFVSNFYYEASTGYFEGPSEQKALLHTWSLSVEEQFYIL
                     FPIYLWLARRYLGRHFLKVTCALTLGGFALSAWGVAYHPRAAFFLAPTRAYELLCGAL
                     VALGVFPRPSRPLIRDGLSLVGLVLIGFAYLFFNSHTPFPGVAALAPCAGTALVLWAG
                     SESSSWAGRVLSARPLVMVGWISYSLYLWHWPVLVLAEYWNIFKLGPWGTTACLAAAL
                     GLAVLSYRWIERPFRSGNALLAARAPLFRAALAAMAVTVAVGGYGTLTHGAPGRFGER
                     PVAHFVGSNWPVCVRGALPLAQSGEVCTVGDKSDLAPSVAVWGDSHADALASAVAEVG
                     RSLHRGILYLSKPGCPPLVDVQKVSPDDGVSETQACEAHTARALEILEQKREVATVVL
                     VARWPYYAQGVGFGADRNHVVRLYSRQDHSALSEQGVGRSLEQTVQRLIGMGKRVLLV
                     EPFPEFAHPVADSLARAALYGKDPTFLRVDETRVSERNRALGEVVRRLEGSVGFRFLR
                     MRDIFCRERVCQPFEGDTPFFVDNNHLGAIGTGRVVPRLLQALRDERFD"
     gene            complement(4083..5207)
                     /locus_tag="VFQ61_06730"
     CDS             complement(4083..5207)
                     /locus_tag="VFQ61_06730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9954178.1"
                     /translation="MHKVLFVASLGFSLIACSGGNRGGAVSGSGGDALGAAGSMGGAT
                     IGSGGMTTPETGPGGSTLGGASTSISGGGAAAGGAAAGGITAGGSGGTIAPGGRTNGG
                     SGGGSAGLGGKTAGGTGSGAAGSPGNGGKSSSGGSASGGTTNGGNSSSGGLTGNSGPC
                     TIWPIGDSITVGHGSDASGFRTGIFAKTVQDNLSVSFIGTESDGPDMFAGRPFPKRHD
                     GYVGYRTDQVLALIQDIASAVKNTPPGVVLILLGTNDAIQGATMADSARDIGKVIDAV
                     FGAFPDAAVALAQITPTNQSDVNMRAEDLNARLPALVESRSAAGKRITLVDMYAAFTR
                     EPAYANKYLKDGIHPNDTGYAVMTEAWYAAIGPALQGCSR"
BASE COUNT         1141 a         1827 c         1693 g          938 t
ORIGIN      
        1 agtcgccgag tatcgacagc gcacggttca aggttttcgt cgtgtcgcgg tcttggtcag
       61 cacgacggcg gggcaactgc aggtccagcg acacatcgac gaggagaagc tcaagacggc
      121 gcgcgtgttc gtggattggg aggaggcgct ggtttggttg cggcagacgc cctcgcgtct
      181 tccttgaggg ttcagagtgc gattgagcta acccttggct ggctgcgcgc gcgccgagcg
      241 tcccatgcgg gcgatctgtc tcaggcggcg gacgtgctgt cggacggcgg cgccagttac
      301 cggcccgcca tggccgagga ggaaggtctc cgtccccgtc tcgagcaacc tctctagttg
      361 atcagcgact tcagccggat cgtcctcgaa aggaggctgc tggcaacgct cggtgaaagc
      421 aatgccgccg agcagaattc ccgacgcgac cagatcgccg acgagcgcga ccgatccggg
      481 cagctcgacg gatagagatc ctgcggtgtg gcctggcgtt ggcagcagct gaccgggtat
      541 accgaaggag gcgagatcca tgggcgcatc gtccacgacg acatccgcgc ggaagggttc
      601 gaacgcttgg aacggcaagc gcgtcttgag aaacgcgcgc ccaaaccagc ccgtagggca
      661 acgggtcacc tgctcttcac cctgtaatac cggcagctca cgccgttgag ccacgatggg
      721 agctccgctc agatcacgca ttcgggcggc atctcccgcg tgatccacgt gtccgtgagt
      781 cacgacgatc aggcgcacgt tgccccagcc gaggcgacgc tccgcgaggg ctcgctcgaa
      841 gcgaggtgcg gtgcccggca ggcccgcatc gacgataatc gctcgctggt ctcgaaagac
      901 gatgtgagca ttgagcatcc cgagcgggag cagaggaacc cttacgatct cggcgccaaa
      961 ggccgtggtc aaagcgctag ccatgcgcca gagtatggat agctggttgc cccgacgcct
     1021 gttccgttcc tacatcacgg ttattcaatg ttggaagtcg tcccgttcgc cgcgccgccg
     1081 gattcgctgc tcgactacgc cgttcgcctc gagcagtgcg acgttctgtg ggttggcgag
     1141 ggggagttcg cgattcgctg cgaacctcag cgccccgacg atacagtcaa gctcctgctg
     1201 aacatcgaag gcagcgctga gctgaaacaa cagggccgct gggcggtgat cgaacgagga
     1261 acctattctt gggtcatcgc agatcagccg ttcgagctcg tttctggcgc cggtagccgc
     1321 caactccaga ttcgctggcc gcgcgcggtg gtcgggcagc gacaccccga cctggatacc
     1381 accccggccg ttacgcgggg cctcgaccat gcgggcgaac gtttcatctg cgggctactg
     1441 aaaacgttgg cccatgaggg ccccaagctc gatcgggggt cacacgaggc tttggtcgcg
     1501 accttgctgg aagcgttggg gttggccaag cggcgagcgt cgcaagaccc gctcgatcag
     1561 cgaatagccc gcgcgcgtcg ggacatcgaa tgtctcctgc acgatcccag catgggcccc
     1621 gtgcaaattg cgaggcgaca aggggtgagt cgacgctact tggacggcct atttcaagcg
     1681 cgttgcgcag aaacattgag cgccgtgatt cgaaggcggc gcctggagcg agcggcagag
     1741 caactcgtgt ccgaagcgtc tcacagcgtc gagtcgatcg cccaccgttg gggatttcgc
     1801 aacgcgagcc actttagcca agccttccgc gaacacttcg cttgctcacc aacggggtat
     1861 cgctcccgct tcccccaccg gccctgaacc agccgcgccc ttgcttctgg cggcagtact
     1921 gacgggcatt tctcaatcga atcgttcgtc gcgcaaagcc tggagtagac gcggaaccac
     1981 gcggccagtg ccgatcgcac cgaggtggtt gttgtcgacg aagaacgggg tgtcgccttc
     2041 gaagggctga cacacccgct cacggcagaa gatgtcgcgc attctcaaga atctaaatcc
     2101 tacgctgccc tccaggcgcc ggaccacctc ccccaacgcc cggtttcgtt cggacacccg
     2161 ggtctcgtct accctcagaa acgtgggatc tttgccgtac aacgcggctc tcgccaatga
     2221 gtccgccacc ggatgggcga actcggggaa cggctccacg agcagcacgc gcttacccat
     2281 tccgatcaaa cgttgcacgg tctgctcgag gctgcgaccc accccctgct cggacaatgc
     2341 gctgtggtcc tgcctggagt atagccgaac aacgtggttg cgatcggcgc cgaacccaac
     2401 cccctgggcg tagtagggcc agcgagccac cagcacgacc gtcgctactt cccgcttctg
     2461 ctccaagatc tcgagtgcgc gggccgtgtg agcttcgcac gcttgtgttt ccgatacccc
     2521 gtcatcgggg gacaccttct gcacgtcgac gagcggcgga catcccggct tcgaaagata
     2581 caaaatcccg cgatgcaaag accggccaac ctccgcgacg gccgaggcca gcgcgtcggc
     2641 gtgtgagtct ccccagaccg cgaccgatgg tgctaagtcc gacttgtcgc caacggtgca
     2701 gacttccccc gactgcgcga gcggcagcgc gcctcgaaca catacaggcc aattggagcc
     2761 tacgaaatgg gctacgggcc gttccccgaa ccgcccgggc gcgccgtgag tgagagttcc
     2821 ataaccaccg accgccaccg tcacggccat ggccgcgagc gcagctcgaa acaacggcgc
     2881 gcgcgcggca agcagcgcgt tcccactgcg gaacggcctc tcaatccagc gataggaaag
     2941 caccgctaac cctagcgcgg ccgccaaaca cgcggttgtc ccccacggac cgagcttgaa
     3001 gatgttccaa tactcggcga gcacgagcac aggccaatgc caaagataga gtgagtaaga
     3061 gatccatccc accatcacga gagggcgtgc cgaaagaacc cttcccgccc agctgctcga
     3121 ctctgaaccc gcccacagaa ctagcgcggt cccggcgcag ggcgctagcg cagcaacccc
     3181 tggaaacgga gtatgagagt taaagaacag atacgcgaag ccgatgagaa ccagccccac
     3241 tagggacaag ccatctcgga tcaggggccg agaagggcgc gggaagaccc cgagcgccac
     3301 caaagctccg cacaacaact cgtaggcccg agtcggcgcc agaaagaacg ccgcccgcgg
     3361 gtgataggca acgccccaag cactcaacgc aaaaccgccg agcgtcaagg cgcaggtcac
     3421 tttcagaaaa tggcgtccga ggtacctgcg cgcgagccac aggtaaattg gaaacaagat
     3481 atagaattgc tcctcgaccg aaagcgacca cgtatgcagg agagccttct gttctgaagg
     3541 tccttcgaaa tacccggtgg aggcctcgta gtagaagttg gagacgaaca gcgtggcggc
     3601 gatcaggctt ccacagtagt cacgaaagtg ttcgggcagc aggagtaacg cggcgagcgg
     3661 cgtcacgacc gcgaacatcg tgaatagcgc cggaaatatc cgacgcaccc ggcgttcgta
     3721 gaacttggcg atcgaaaagc ggccctggga gagctctcgg tcgataatcc ccgtgatcag
     3781 aaagccagag atcacgaaga agacatcgac gcccgcgaag ccgcccgcca accgagaaat
     3841 gcctgcatga aacaatacga ccgggatcac ggccagggca cgcagcccgt cgatatcggt
     3901 acggtggcgc tcgaccatcg aaccctccat gggctcgacc acccgcgagt tcactccaac
     3961 ctcggagtga gtcaccagta gtcgagacca gccgggagct taagccggga tccaccacaa
     4021 aagcatggta aaacgattgt gatggatgca ccaaacgccg gtgcggtagg cagagtgcta
     4081 gttcaacggc tacagccctg aagagccgga ccgatcgcgg cgtaccaagc ctcggtcatg
     4141 accgcatatc cggtgtcgtt cggatgaatt ccgtctttca ggtacttatt cgcgtaggcc
     4201 ggctcgcggg tgaaagcggc atacatgtcg accagggtga tgcgcttgcc ggccgcggac
     4261 ctcgactcga cgagggcggg caaccgggcg ttcagatctt ccgcccgcat gttcacgtcg
     4321 ctctgattcg tgggcgtaat ctgcgccaaa gcaacggccg catccggaaa ggcgccgaac
     4381 accgcgtcga taacctttcc aatgtctcgg gcggagtcgg ccatggtagc gccctgaatg
     4441 gcgtcgttgg tgccgagcag gatgagcacc actcccggag gcgtattctt gaccgcggaa
     4501 gcgatgtctt gaatcagagc gagcacctga tcggttcgat aacccacgta accgtcatgg
     4561 cgtttcggga acggcctgcc cgcgaacatg tccggcccgt ccgattcagt accgatgaag
     4621 ctcacactca ggttgtcttg aacggtcttc gcgaaaattc cggtgcgaaa cccggatgcg
     4681 tcagagccat ggccaacggt aatcgagtct cctatcggcc agattgtgca cgggccggag
     4741 ttgccggtga gacctcctga agaggagttc cctccattgg tcgtgccccc gctcgcgctg
     4801 ccgccactgc tgctcttccc accattgccc ggcgagccag ccgctccgga gccggttccg
     4861 cctgcggtct tgccgcccag gcctgcactt ccacctccgg agccgccgtt ggttcgcccg
     4921 cccggagcga tcgtcccacc ggagccgccc gcggtgattc caccagctgc ggctcccccg
     4981 gctgcggctc caccgcccga gatactggta gaggctcctc ccaacgtgga tccgcccggg
     5041 ccagtttcgg gagtggtcat gccgcccgaa ccgattgttg ctccgcccat cgagcccgca
     5101 gctccgagcg cgtcaccgcc ggagcccgac acggctccgc cgcgattacc tccggaacaa
     5161 gcaatcaggg agaatcctag actcgctacg aaaaggacct tgtgcattcg agcgcctcgc
     5221 ttttccagga attttccaga gcccacggtt cgccgcctcg tagagaaggt caagcatact
     5281 tcgcgtccca acgcccgcgc cccagaacga ccgcacgact ctgaaattca tccctcggag
     5341 ttcgaatggc gacggttgca cccgacacct gcgcacccac tacgctctcc aaggaacatg
     5401 tctccttctg cagccgacag gatgcagcaa cgctgcttac cgcacgcaaa gctccgccgc
     5461 tgcgttactc cgcgagcatt ggagtggacc attccttcca gtccgtcgcc cgttgagtgg
     5521 cggcaaagcg cgcacgttgg cgcccagcgc gataatggcg atctgaattg catgacgtat
     5581 gctcctcgag ccgcggatc
//
LOCUS       DASTKU010000176         4038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_925390, whole genome shotgun sequence.
ACCESSION   DASTKU010000176 DASTKU010000000
VERSION     DASTKU010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925390"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1382
                     /locus_tag="VFQ61_06735"
     CDS             216..1382
                     /locus_tag="VFQ61_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HET9954179.1"
                     /translation="MIPAKTGRTAVSKTRQILSLTELLRKRDTVINCGMFSRVFSRVT
                     RALPALPALTLACTLAACDAGRSSEPKKPACCNTSDEWKRPTTQTPLEAHGKLSVSGS
                     RLTDEKGQPVQLKGVSSMWLNWENDGYAESLDALTWMRDNWNISLIRAAMGISPGGAY
                     LTNPDKAKTQVTTIVDNAIAAGVYVIVDWHSHEALNEQAKAVEFFTEMAQKYVGVPNV
                     IFETFNEPLGVDWSTQLKPYHEAIVSAVRAIDPDRILILGTPHWSQDVDIAALDPVQG
                     GNLMYTLHFYSCTHGGVLNKGANAVARGLPLFVTEWGATNADGGLDGKVCEDEAATWL
                     EFLEDEGIGWAAWKLDGCEPDSTCLLVPGTPVNGGWTSEYLHGHAPFVRDWLRR"
     gene            complement(1519..2421)
                     /locus_tag="VFQ61_06740"
     CDS             complement(1519..2421)
                     /locus_tag="VFQ61_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9954180.1"
                     /translation="MATQSSSTPRALFEHVEHDRDLKIVFRDVVGRALKYPFHVHPEI
                     ELTHIVRGSGLRYVGDSIQPFGDDDLCLIGPMTPHCWLSEGARLEPIHARVIQFLPES
                     VCGERGSSAAFRPLQQLFQRAGAGLRLRGETQRRAAAQMRALFTDPGRPLDRYLGLLS
                     LLTDLSRTEEWDELALNQDSVSVDTASAETVNRLLALIHEHAADPALSFGDLARHVGM
                     SRATLGRAFPRLFGKTFVKYLSEVRILKACTLLSETDRSVCDIALSTGFGSLSNFNRQ
                     FRTLKGTTPLRYRQFAARRSVARG"
     gene            2592..>4038
                     /locus_tag="VFQ61_06745"
     CDS             2592..>4038
                     /locus_tag="VFQ61_06745"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9954181.1"
                     /translation="MLYRAPMARQSTGDTSGRDMPGRRESTSRGLFFKRGAWDPTSLF
                     RLGSVPGDPEDVRAFIGRRVSSFLGFSCFMWGFAWVIAIFIDALTRPDLIALSGLNGL
                     RLVMHLLVVLALLAAWVSTRRRDLSLRALAALDFLGTELQALALGMLLVYSLPIIQYR
                     PDISMILGLTYLLIGRAALVPSTASRTALIASTASVFMLLATWWVHHSADQAGTSIAH
                     FYPGEKGTPLVYTAWGALFCALTTISATFVSAVIFGLQREIARVRKLGQYVLENEIGS
                     GGMGIVYRARHAFLRRPTAIKLLRPERAGSAAIQRFEREVQITSQLTHPNTVAVYDYG
                     RTPDGTFYYAMEFLDGIDLDRLVAIDGPQPEARVRHVLRQVASALSEAHAKGLIHRDI
                     KPSNIVLCERGLSQDFVKVLDFGLARELETLAPGLSTLGQVVGTPLYMSPEQVTQPEK
                     IDERVDLYALGAVGYFLLCGEPVFTGDTTVEV"
BASE COUNT          774 a         1248 c         1271 g          745 t
ORIGIN      
        1 ggcgcccatg ccgccttccc cgatcaggcg gatgattcga tacttgtcgt cgatgaaatc
       61 gccgggattg agccgcatat gcccgaatcg aaactagcac agagctccgt ctttgatgct
      121 ccgttcgcac gaagagcgtg cagaacctca accaagcgct catgtaacgg cccggttcca
      181 gttatactgc agcatacctg cccccagagt cttcgatgat cccagcgaaa acgggccgca
      241 cggccgtgag taaaactaga cagatcctga gcctcacgga gttgctcaga aagcgcgata
      301 ccgtcatcaa ttgcggcatg ttttcgcgcg ttttctcgcg agtcactcgt gctctgcccg
      361 ccttacccgc acttacgctt gcctgcactc tcgctgcttg tgacgcgggt cggtcgagcg
      421 aacccaagaa gcctgcgtgc tgcaatacgt cggatgagtg gaagcgtccg accactcaga
      481 ctccgctcga ggcccacggc aaattgagcg tgtccggctc ccgcctcacc gacgagaagg
      541 ggcagcccgt gcagctcaag ggcgtgagca gcatgtggct gaattgggag aatgacgggt
      601 acgcggagag cctcgacgcg ctcacctgga tgcgtgacaa ctggaacatc agcctgatcc
      661 gtgcggcgat ggggatcagc cccggcggcg cctacctgac caaccccgat aaggcgaaga
      721 cccaggtcac gacgatcgtg gataacgcga tcgcggccgg cgtatacgtg atcgtcgact
      781 ggcattcgca cgaggccctg aacgagcagg ccaaggcggt cgagttcttc acggaaatgg
      841 ctcaaaagta cgtcggagtt cccaacgtga tcttcgagac gttcaacgag ccgctcggag
      901 tggactggtc gactcagctc aagccgtacc acgaagcgat cgtgtcggcg gtgcgcgcga
      961 tcgaccccga tcgcatcctc atcctgggca ccccacattg gtctcaggat gtagacattg
     1021 ccgctttgga tccggtccaa ggcggcaatc taatgtacac gctgcacttc tattcctgca
     1081 cgcacggcgg cgttttgaac aagggcgcga acgccgtggc caggggccta ccactgttcg
     1141 tcacggagtg gggcgccacc aacgcggacg gtgggttgga cggcaaggtc tgcgaagacg
     1201 aagcagctac ctggctcgaa ttcctcgagg acgagggcat cggatgggca gcttggaagc
     1261 tcgacggctg cgagcctgac tcgacctgct tgctcgtgcc cggcacgccc gtgaatggcg
     1321 gatggacgag cgaatacttg cacgggcacg cgccgttcgt gcgtgattgg ctgcggcgct
     1381 gaaccgcacg cgcgcaaatg aaagaagcgg gagtgagctt gctcactccc gctttcgttt
     1441 tgtcccctcg cctccttgcg gacgctgctc gtctcactcg aaggtgcggg taattgaccg
     1501 agactggcgc gaccccgctc agccgcgggc cacgctgcgg cgggcagcga actgccgata
     1561 gcgcagcggt gtggtgccct tcaacgtccg gaactgacga ttgaagttgg acaagctccc
     1621 aaagcccgtc gagagcgcga tgtcgcagac gctgcgatcc gtttcggaca gcagcgtgca
     1681 cgccttcaag atgcgcacct ccgacaggta cttcacgaaa gtcttgccga acaagcgcgg
     1741 gaacgcgcgc cccaaggtcg cgcgggacat gcctacgtgg cgggccaagt ctccgaacga
     1801 gagcgcggga tcggcggcgt gttcgtggat gagagcgagt agacgattca cggtttcggc
     1861 agacgccgta tcgaccgaga ccgaatcctg gttcagcgcg agctcatccc attcctcggt
     1921 ccgagagaga tcggtgagca gactgagcaa tcccaggtag cgatccaaag gtcgccccgg
     1981 atcggtgaac agcgcacgca tctgcgccgc ggcccggcgc tgcgtctcac cccgcaagcg
     2041 cagaccggct cccgcacgtt ggaagagctg ctgcaagggt cggaaagccg cggaagagcc
     2101 gcgctcgcca cagaccgact ccggcaggaa ctggattacg cgcgcgtgaa tgggctcgag
     2161 ccgcgcgccc tcggagagcc aacagtgagg cgtcatcggt ccgatcaggc aaaggtcgtc
     2221 gtcgccgaac ggctgaatgc tgtctcccac gtagcgcagc cctgaccccc gcacgatgtg
     2281 cgtcagttcg atctcgggat gcacgtgaaa cgggtacttc aaagcccgtc caaccacatc
     2341 gcgaaacacg atcttgaggt cacggtcgtg ttcgacgtgc tcgaacaagg cgcgcggagt
     2401 ggaacttgat tgagtagcca attcaaacct cgagaatagc ggaaccggag ccgagcgatg
     2461 tctcacggct cgagaccatg tccgaaaacc aagcccctcc tgtcccgggg ctcacatcgg
     2521 ccggcagggc cgggccgagg caatcgggac gatcggggga atcaggtgat gcaagggaaa
     2581 gcccgctgac catgctatac agggctccga tggcgcggca gtccacgggc gacacgtcgg
     2641 ggcgcgacat gccagggcgg cgcgagagca cttcgagagg cctgttcttc aagcgcggag
     2701 cgtgggaccc cacgagccta tttcgcctag gcagcgtgcc cggggaccca gaagacgtgc
     2761 gcgccttcat cggtcgccga gtgagctcgt tcctgggttt ctcgtgtttc atgtggggtt
     2821 ttgcgtgggt gattgcgatc ttcatcgacg cgctaactcg gcccgacctg atcgccttga
     2881 gcggcttgaa cggcctgagg ctggtgatgc acctgttggt cgtgctcgcg ctgctcgccg
     2941 cgtgggtctc gactcgccgt cgtgatctct cgctgcgagc cttggccgcg ctcgacttcc
     3001 tgggcacgga gctgcaggcg ctcgcgctgg ggatgctgct cgtctactcg ttgccaatca
     3061 ttcagtatcg tccggatatc tcgatgatcc tggggctcac gtacttgctg atcgggcggg
     3121 cggcgctggt tccgagcacc gcttcccgca ctgctctgat tgccagtacg gcgagtgtgt
     3181 tcatgctctt ggcgacctgg tgggtgcacc acagcgccga tcaggccggc acttcgattg
     3241 cccattttta cccaggagaa aagggcacgc ctttggtcta cacggcgtgg ggtgctttgt
     3301 tctgcgctct gacgacgatc tctgcaacct tcgtctcggc cgtgatcttc ggcttgcaac
     3361 gtgagatagc ccgagtgcga aaattgggcc aatacgtgct cgagaacgaa atcgggtcgg
     3421 gcggtatggg catcgtctat cgcgcccgac acgcattcct gcgcaggcct accgcaatca
     3481 agttgcttcg ccccgaacgc gcaggaagcg ccgccatcca acgcttcgag cgcgaggtgc
     3541 agatcaccag tcagctcacg catccgaaca cggtcgcggt ctacgactac gggcgcacgc
     3601 cggacggcac gttctattac gcgatggagt tcctggacgg catcgatctc gaccgtctgg
     3661 tcgcgatcga cggtccccaa ccggaggcac gcgtgcgaca cgtgctgcgc caggttgcga
     3721 gcgcgctctc cgaagctcac gcaaagggct tgattcaccg ggatatcaaa ccctccaaca
     3781 tcgtgctctg cgagcgcggt ctatcccagg acttcgtgaa ggtgctcgac ttcgggctcg
     3841 ctcgcgagct cgaaacgctg gcccccggac tgagcacgtt gggacaagtc gtcggcacgc
     3901 cgctctatat gtcgcccgaa caggtaactc agcccgaaaa aatagatgag cgagtcgatt
     3961 tgtacgcgct cggggcggtc ggctatttct tgctgtgcgg ggagcccgta tttacgggcg
     4021 atacgacggt cgaggtgt
//
LOCUS       DASTKU010000177        11173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_925505, whole genome shotgun sequence.
ACCESSION   DASTKU010000177 DASTKU010000000
VERSION     DASTKU010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11173
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925505"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1155)
                     /locus_tag="VFQ61_06750"
     CDS             complement(19..1155)
                     /locus_tag="VFQ61_06750"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HET9954182.1"
                     /translation="MAVSIFGLLLVACGVEEHRHDQKEVGMGGMVFRPPPANEGGAAS
                     PSGGAGPFAGGASSGFGGATAGGAGAVNGGGFPVGGLTGIGGGGNPGTPPSGGTPNGG
                     TAGTSAGGASAGNSAAGMAATGGRSGVTVDINGTVLPKEDVIAFVHIGHSNMFGYGSS
                     PSASRAYHFTDVDLRAWQYHDGNWTPARERTAGAGTTGAGPGTSLVKQAVALAPNRYF
                     VSLGYGVGSAYCSQFLRGGLYYDEFMAGPRALKGKVTFGAIVIMLGITERHGTDQDIA
                     NYPNCINEVVTAIRTDLGEPNLPLLLCDYEMGATGSELSPTGEFGQKMIPKIHDVPNV
                     VSRSAIVPTDGLSMQDDHHFNLDGQKEWSRRVLTTMKDKGWFPW"
     gene            complement(1286..3229)
                     /locus_tag="VFQ61_06755"
     CDS             complement(1286..3229)
                     /locus_tag="VFQ61_06755"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9954183.1"
                     /translation="MLQFAAIFAGVFAVCLTASTDGDVFWHLAAGQEIVARKALLRVD
                     IFSLSARDRLWIDVHWLFQVLCAWGHQLGGLRALVLGKAFVVASGAMLLAGVVRRLAG
                     AAALPFFALALLGALLSVRDLLLLRPTMFTLLFVAAFVYVIESARAERSPRRLWLLPL
                     LQVVWVNIQGLFALGPAIIAAYWLGWMLESRLRRADWSPFAVDRVRCRDAALRRVLAL
                     TLAGSLLACVVNPFGIRAVLLPTELLRRLTPSSANAFSNDVIENVPPFLLEQQTGQFW
                     HLKWFILALLLVVLVTGRRLRAHHCLLVGGFLVLALTANRNVLLFYWVATPLMAAYSF
                     AAARRRWARVPALFAGLGGLTGAALLTVFAVVIQAQREEPSLDAPAPFRVPELSARWI
                     AEQGGKSRIFAADHYAGYLIWKLFPNHVPYIDTRLILRSEREFEEYLSSVDEPARFDA
                     FAERWRFDYVVLPTSYPERYLALLRHLYESSSWRLVLSDGSETLFARRDRGNYPELNL
                     NAASTTARLLGDFEQRYADGPVRADARRQLATLQLALGHPERAEQALSSNLDVPSMAL
                     RARARLAQGDPAGAGRVALQALSVDPNHVRALNLLAAISIERGEIGKAMGYLRHAVRA
                     NPFDSETLAILRTLQEAPHDAIN"
     gene            complement(3272..4342)
                     /locus_tag="VFQ61_06760"
     CDS             complement(3272..4342)
                     /locus_tag="VFQ61_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954184.1"
                     /translation="MSGSPRRRDSGARRAKLFIGLVAGALGATALYPLYARQRELGRG
                     ESLLRAATRRVSTSSAQASGRLAQDALSLRRERLDRLLSTWEAIGGCGAGSSAGAGSS
                     VKWIGRSVRGGLFSAQLMTSYLRLPAQYNLSGGYTLSMVAQVTRDLGEKWNVGVAVPY
                     LYKYYSDYYTLPLDISNAGLGDVSLLLTRRFGAINDTLVTATLGLPTGNHDARFRNDL
                     LTQDKQLGHGRVTGSLTVDHVFDEVWGLIVLGGLASYRGGTNELGNYRAPMGSLYGHA
                     GYYLGPFVPALGLTFNAFAGRDRDRTLEQHSPLYTLAGSGSIEWSSDYVAVLLGGSFP
                     LRLPEFTTEPWIVAVGVAVSPF"
     gene            complement(4339..4779)
                     /locus_tag="VFQ61_06765"
     CDS             complement(4339..4779)
                     /locus_tag="VFQ61_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954185.1"
                     /translation="MFTRSQFCRAFFATPILVAVLGWPAASIADDAVQKVTAKLVEIP
                     SKFPPDDLYDYAYVMRYEVIGGPLDRSSILVAHYKPRQPRSKIKDKMKSFVSGKVRSF
                     AVGDVHKLELSGDLKKIWKGALVDEFAATDRKSVRYWCLVADPA"
     gene            5339..6658
                     /locus_tag="VFQ61_06770"
     CDS             5339..6658
                     /locus_tag="VFQ61_06770"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9954186.1"
                     /translation="MAVDYLRAPPRHRLDPHRHVVTAWCMPLPLAPLGVSGVHSQVAI
                     GIVASASTMVERASRIGWIDVLRGWAVLSVILLHLNIRVPFAASRIGAGLPGPIIKAV
                     FWSGYHGVIVFFVISGFLITTMALERWRALDHIPLREFYVRRFARILPCLLLFVVVQS
                     ALQLALGEPFSSQTSPTSLPRTVLAALTFHLNWLEARDGYLPGAWDVLWSLSIEEAFY
                     AFFPILCVLIRWPSALVVALGGFVLAGPFARSSSAANEIWADHGYFSCMGEIALGCLT
                     ALYAARVRTSPERARLLAMGGAGLMVFVIFFRRALFDWGISPRGLHVNLLALGTALVI
                     LSVHSRPDWNVSSGGAGLRGLRRLGRISYEVYLSHLFVILPAVVVFEALGSPKSAIVP
                     FDLLNLVATGLVGAWLAKYFSEPLNRRLRAALLLPESQRSDRQLLDE"
     gene            7045..7974
                     /locus_tag="VFQ61_06775"
     CDS             7045..7974
                     /locus_tag="VFQ61_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET9954187.1"
                     /translation="MKTSFDRFLNRNFALALGAWALVTGCASSAEETLDRDETASSTS
                     APIIGGQNAVAGQHKWIANVLVNDGGVWRHQCGGALIAAQWVLTAAHCIANSGGWYSN
                     TQIRVRLGDYDQTVSEASEQLKNVTTVWIRDGYNADPNVSPSNYDMALIKLSSAATLN
                     SNVNTIALATQTPPTSCPPSNLSCNDVIAAGWGATAPLNPPPPTESSNILQRATIKLR
                     SQSTCNGAEVGAIRSATGSPGLKFDELCVGSSNGNPGPCKGDSGGPLVRAKSGGGYEL
                     VGVSSWGPLYCTSYAVYGSVPAHTDWIQWVLTH"
     gene            8220..8924
                     /locus_tag="VFQ61_06780"
     CDS             8220..8924
                     /locus_tag="VFQ61_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET9954188.1"
                     /translation="MIVDTIATGSKLNWLATCWGALLLVASAPRAAAAPSCDELSISA
                     ARALPLGSQVTIEGTVSTPSGVFASSFFDAGFGIQDRRAGIYVSVAANPEVVPGDRLR
                     VTGVLADSYGLLVLIPNGATDIQEVGAGHPPAADRVDTGSIGESTEGVLVAVKGRVTQ
                     APVSDLPYGYKFEVDDGSGELQIFVNVDTGIDLSSIAVGRKIKVTGLSSQFEDHYEID
                     PRFPQDVVLLPRNDGP"
     gene            complement(8981..10051)
                     /locus_tag="VFQ61_06785"
     CDS             complement(8981..10051)
                     /locus_tag="VFQ61_06785"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9954189.1"
                     /translation="MRAGLTLASGCSADEDRPTPGAEAAWVGRTANYGAAPVAVLIDH
                     GSELCSAVVVSPRLILTAGHCLLQGSAALRVSFDSPAGAIENDGRLLDARWTRNFDPL
                     TLQDDLVLAVLSHETAFAPISLAHAAPGEHDVLTVVGFGGSPEAPFLRSSGAVMSQRI
                     DPETGLLRLSPAPARVCHGDSGAPIFNARGELVAIVSRGPAECDAEFGATLLTDEHRE
                     WLQQFALEVEARAAPGKRCWFDAQCEDGQCVSPVDAPGRRYCAPNCGPTIRCPAPMTC
                     DMTQGRCMLPRPSPGALGSACTEAWDCGVGVCGQPLNSNQTSQCLQPCAGLDLGCPPE
                     QRCVPAQLRGSPSPWTHVCIDR"
     gene            complement(10127..11059)
                     /locus_tag="VFQ61_06790"
     CDS             complement(10127..11059)
                     /locus_tag="VFQ61_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954190.1"
                     /translation="MKSLLHATSTTGPRRADAPRSRTWAPRKTVLVGALWILTVACAA
                     EPPGTPLESGSVQDVGGSGTGTEALSLTENSDTALTGEFASEAGTAHFDSQFDATGQA
                     VSVVVIGETTIEWRYDNQSGRVTVDGRGSVLTEQSLTVFAELSKQLYAALDAEGTAKG
                     RLPTHKGVLAAQVSWLAAGQPGTVIDAFSSGPSGTVSGEVAYASFTDDGVTCLRKGVS
                     TRYWAKADGGNSRSAYFVVNDTQSCNGSVKGCGNWSGSCMGQCGEGCQSSWKNGSFQD
                     CFDHDFCTYFYRDCGAQFRDAADDYAASLFDRCN"
BASE COUNT         2144 a         3504 c         3402 g         2123 t
ORIGIN      
        1 gagaagacca tcgctgaatc accaggggaa ccaacccttg tccttcatgg tcgtcagtac
       61 gcgtcgggac cactccttct ggccatccaa gttgaagtgg tggtcatctt gcatgctgag
      121 gccatcggta ggcacgatcg ccgagcgcga cacgacattg ggcacatcat ggatcttcgg
      181 aatcatcttc tggccgaact caccggtggg agaaagttcg ctgccggtgg cacccatttc
      241 gtagtcacac agcagcaacg ggaggttcgg ttcgcccaag tcggtgcgga tcgccgtgac
      301 gacttcgttg atgcagttgg gatagttcgc aatatcttga tccgtaccgt gccgctcggt
      361 gatcccaagc atgatcacga tagctccgaa cgtgaccttg cccttcaacg ctcgagggcc
      421 ggccatgaat tcgtcgtagt acaggccacc ccgcaggaac tgcgaacagt aggcgctgcc
      481 cacgccgtag ccgagtgata cgaaatagcg attcggcgcg agggcgaccg cttgcttcac
      541 caaagaagtg ccggggccgg ccccggtcgt gccggcgccc gccgtgcgct cgcgggcggg
      601 ggtccagttc ccatcgtgat actgccaagc gcgcaggtcg acatcggtga agtgataggc
      661 cctcgaggcg gacggcgagc tgccatatcc gaacatgttg gagtggccga tatgtacgaa
      721 agcgatgaca tcctctttcg gcagcaccgt gccgttgatg tccacggtga ctccggagcg
      781 cccgcccgtg gcggccattc ccgcggcgga gttgcctgca gacgcgccac cggcgctggt
      841 acccgcggtt cctccgttag gggtcccacc gcttggagga gtgcccgggt tccctccacc
      901 accgatgccc gttagcccgc ccacaggaaa gccgccgccg ttcaccgcgc cagcaccgcc
      961 tgccgtcgcg ccaccgaagc cactgctggc accgcccgca aaaggtccgg ctccgccgct
     1021 cgggctggcg gcgccgccct cgtttgccgg aggcggtctg aacaccatgc cacccatgcc
     1081 gacctctttt tgatcgtggc ggtgttcctc gaccccgcac gccaccaata gaaggccaaa
     1141 tatggatact gccaaagcct tctcggaaag tagcctcgga gatcgcggct ctggccttcg
     1201 cttcagcctc gagttcgcgt catcttctgt tatgaggccg gcgtctacgc acaccgaacg
     1261 agatcgatat ctgagcggtc gagcactagt tgatcgcgtc atgtggcgcc tcctgcaagg
     1321 tacgtagaat cgcgagtgtc tcggaatcga acggattcgc acgcaccgcg tgtcttaagt
     1381 aacccatggc cttcccgatc tcgccgcgtt cgatcgaaat agccgccaag agattcagcg
     1441 ctcgaacgtg gttcggatcg acgctcaacg cctgcagagc gactcgccct gcacctgccg
     1501 ggtccccttg ggcaaggcgt gcgcgcgcgc gcagcgccat cgaaggaacg tccaggttcg
     1561 aagacagagc ctgctcggct cgttccggat gacctagcgc gagctgaaga gtcgcgagct
     1621 ggcgccgtgc atccgcgcgc acggggccgt ccgcgtaacg ctgctcgaag tcacccaaca
     1681 gtctggcggt cgtgctcgcc gcattcaagt tcagctcggg atagttcccc cgatcgcggc
     1741 gagcgaacag cgtctcgctg ccgtcgctga ggaccagtcg ccagctcgaa ctctcgtaca
     1801 agtgccggag cagagccaag tagcgttcgg gataggaggt gggcaataca acgtagtcga
     1861 agcgccagcg ctcggcgaac gcatcgaagc gggcgggctc gtcgacgctc gacagatact
     1921 cctcgaactc tcgttcggat ctcagaatca aacgcgtgtc gatatacggc acgtgattgg
     1981 ggaagagctt ccagattaga tatccggcgt agtgatcggc cgcgaaaatt cgacttttgc
     2041 cgccttgctc cgcgatccag cgcgcggaca attcgggtac tcggaaggga gctggtgcgt
     2101 cgagcgaggg ctcttcgcgc tgcgcctgaa tcaccaccgc gaacaccgtt agcagagccg
     2161 ctcccgtcaa gccgcccaat ccagcgaaga gcgctggcac tcgcgcccag cgccgccgcg
     2221 cggcggcgaa cgagtaagcc gccatgagcg gcgtcgcgac ccaatagaac aagagcacgt
     2281 ttcgatttgc ggtcagcgcg agcacgagga atccgcccac cagcaagcag tggtgggcgc
     2341 gcaagcgacg cccggtgacg agcacgacaa gcaacaaggc gagtatgaac cacttcagat
     2401 gccagaattg gccggtctgt tgctccagca gaaagggcgg cacgttctct attacgtcgt
     2461 tcgagaaggc gttggcggag ctaggggtca agcgacggag tagctcggtc ggcagcagca
     2521 cggcacggat gccaaagggg ttcaccacgc aggccagcag cgagcccgcg agcgtgagcg
     2581 ccaagactcg gcgtaaggca gcgtctctgc acctcacccg gtccaccgca aacggagacc
     2641 agtcggcgcg cctgaggcgg ctctccagca tccacccgag ccaatacgcg gcgatgatgg
     2701 ccgggcccag cgcgaatagg ccttggatgt tgacccaaac tacttggagc agcggtaaca
     2761 accaaaggcg ccgcggcgac cgctccgctc gcgccgactc gatcacgtaa acgaatgcgg
     2821 ccacgaacag cagcgtgaac atggtcgggc gcagcagcag gagatcgcga accgagagca
     2881 gggctccgag cagagcgagg gcgaagaagg ggagagccgc ggctcctgcc aatcgacgca
     2941 cgacgccagc caacagcata gctccgctcg cgacgacgaa cgctttgccg agcaccaacg
     3001 cgcgcaatcc accgagctga tggccccagg cgcagagcac ctgaaacagc caatgtacgt
     3061 cgatccaaag ccggtctcgc gcgctgagtg agaaaatatc gactctgagc aatgcctttc
     3121 gcgcgacgat ttcctggccg gccgcgagat gccagaaaac atcgccgtcc gtggacgcgg
     3181 tcagacacac cgcgaacacc ccggcgaaga tcgcggcgaa ctgcagcatc gttcgtgtct
     3241 ggtgggaggc gtgcgcgcgc gacattgctg ctcagaaggg cgacaccgcg acgccgacgg
     3301 ccacgatcca cggttcggtc gtgaattcag gcagccgcaa tgggaacgag ccaccgagta
     3361 agacggccac atagtcgctc gaccactcga tgctcccgct accggccagg gtgtaaagcg
     3421 gggagtgctg ctcgagcgtg cgatcccgat cgcgccctgc gaaggcgttg aacgtcaacc
     3481 ccaacgcggg cacgaaaggt cccaggtaat aacccgcgtg cccatacagg ctccccatcg
     3541 gtgcacggta gttgccgagc tcgttcgtgc caccgcggta gctcgcgagc ccgccgagca
     3601 cgatcaatcc ccaaacctcg tcgaagacgt ggtccaccgt cagcgagccc gtaacgcgac
     3661 cgtgtccaag ctgcttgtct tgggtcaaaa ggtcgttacg gaagcgagca tcgtggttgc
     3721 ccgtaggtaa ccccagtgtc gctgtaacca gagtgtcatt gattgcgccg aagcggcgcg
     3781 tgagcaacaa gctaacgtcg cccaaacctg cgtttgagat atcgaggggt aatgtgtaat
     3841 agtcgctgta gtacttgtag agatatggga ccgcgacgcc cacgttccac ttctcgccga
     3901 gatcgcgcgt gacttgcgcc accatcgaca gcgtatagcc tcctgacaag ttgtattgcg
     3961 cgggcagcct caagtagctc gtcatgagct gtgcggaaaa gagccctccg cgcacgctgc
     4021 gcccgatcca cttgacgctc gacccggccc ccgcagaact gcctgcaccg cagccgccaa
     4081 tggcttccca tgtggacaaa agccggtcca ggcgctcacg acgcaagctc agggcgtcct
     4141 gcgcgaggcg gccggatgcc tgggcactcg aagtcgagac gcgccgggtc gccgcacgca
     4201 gcagcgattc cccccgtccg agctcgcgct gccgagcata gagagggtag agcgcggtcg
     4261 cgccgagcgc gccggcgact aaaccaatga atagcttggc gcggcgagca ccgctgtcgc
     4321 gcctccgcgg cgagccactc atgcggggtc ggccaccaaa caccagtagc ggacgctctt
     4381 acgatcggtc gccgcgaact catccaccag ggcgcccttc cagatcttct tcaggtcgcc
     4441 gctcagctcg agtttgtgca catccccgac ggcaaagctt ctgaccttgc cgctcacaaa
     4501 tgacttcatc ttgtctttga tcttggagcg cggctgacgt ggcttgtaat gcgctactag
     4561 gatcgaggaa cggtcgagcg gaccgccgat gacttcgtaa cgcatcacgt aggcgtagtc
     4621 gtagagatcg tccggcggga acttggatgg gatctccacc agcttggccg ttaccttctg
     4681 gaccgcgtca tcagcgatcg aagccgcggg ccaacccagg accgcaacga gaatgggggt
     4741 agcgaagaac gctcggcaga actgagatcg agtaaacaac agcgactccc taaaacgatt
     4801 tggaccgtga atacgactgg ggcgggtcag ggaattagaa gaggcagtgc agaggtgctg
     4861 gttcaatcag cgcgcccggc gtggtgtcgg acagcgctcc tgaagaaata ccggcgagac
     4921 ggtttctgtc gacggactgg ttctccgggg ttcgccagag cagtgcgcgc ggaatgagtg
     4981 ttccgtcaaa gtgcgcgaac aaatccggat cggagtttta attttatcgc gattcagaaa
     5041 tgccgagtgt tacgcgctcg tgcgcactcc tcgcggtgat gaaccacctg acattttcat
     5101 gcgattcgaa cgaatttctg cctggagaac acccgctgcg gatacacgcc cggaactgta
     5161 actggcgagt gcgtgaatcg agatccatct ctctcgccga agtctccgac ccgaagcggc
     5221 tgaccatgtg gcggccgccc tcttacatta cgaaagcaga ggaacgagcg caggaaggag
     5281 ccaccggcag gcggctcatt tagcgagcta gaggaggcga gttcgttctc ggccgtcgat
     5341 ggctgtcgat tatttgcgtg cgcctcctcg tcaccgtctc gacccacacc ggcacgtcgt
     5401 gaccgcgtgg tgcatgcctt tgcctctggc tccgctcggg gtttcgggcg tccattctca
     5461 ggtcgcgatt ggcattgtag cgtcggcatc cacgatggtc gagcgtgcgt cgcgaattgg
     5521 ttggatcgat gttctgaggg ggtgggccgt gctgtcggtg atcctgctgc acctcaacat
     5581 tcgtgttcca ttcgcggctt cacgaatcgg ggcggggctt ccggggccga tcatcaaggc
     5641 cgtgttctgg agcggctatc acggagtgat cgtgttcttc gtgatttccg ggtttctgat
     5701 cacgaccatg gcgctggagc gctggcgcgc cctagaccac attccgctgc gcgagttcta
     5761 cgttcggcgc tttgctcgca tcctgccgtg tctgttgctg ttcgtcgtcg tgcaatctgc
     5821 actccagctg gcccttgggg agcccttcag ctcgcaaacc agtccgactt cactgccgag
     5881 aaccgtgctc gccgcgttga cctttcactt gaattggcta gaggcccgcg acggctacct
     5941 accgggcgct tgggatgtac tgtggtcgct gtcgatcgag gaggcgttct acgccttttt
     6001 cccaattctc tgtgtgctga tccgttggcc gtcagcactc gtcgttgcgc tgggtggctt
     6061 cgtgctggcc gggccattcg cccgaagtag ctcggccgcg aacgagattt gggcagacca
     6121 cggctatttc tcgtgcatgg gcgagatcgc actcggatgc ttgaccgccc tctacgcggc
     6181 ccgcgttcgg acctcgcccg agcgcgcacg actgctcgcg atgggtggcg ccggactcat
     6241 ggtgttcgtg atcttcttcc ggcgtgccct gttcgattgg gggatctctc cgagaggcct
     6301 tcacgtcaac ctgctcgcgc tgggcacggc cctagtcatt ctctcggtgc attctcgccc
     6361 agattggaac gtgagctcgg gcggggccgg ccttcgcggg ctgcgcaggc ttgggcgcat
     6421 tagctacgag gtgtatctga gccacctttt cgtcattctg cccgcggtgg ttgtcttcga
     6481 ggcgctcggt agcccgaaat ccgcgatcgt acccttcgat ttgctcaatc tagtcgcgac
     6541 ggggctagtt ggggcgtggt tggcaaagta cttctccgag ccattgaatc gccggctacg
     6601 cgcggctctt ttgctgccgg agagccaacg aagcgaccgg cagcttcttg acgagtgatc
     6661 tgagttcacg tgcggtgatt cccattcacg agttcatagc acctgttgga gcagggttat
     6721 ttccgcagcg cggtgaagca cccgcgcgcc aatcgcttgg atgccatcag tctgcggttt
     6781 gtatttcgaa ctcacaaact tcgtgatctt cgcgcccagc ggtattaatt cagacacagg
     6841 tattccgtcg catatgcgga gccgtcttgc gcacgatgcg ttcgttctct cttttgatct
     6901 gcgctcctcg gatctgccat cagattcagt tccttcgttt ccgtttggca atctgtcgct
     6961 tgcattgcgt gattattctc gtgtactgaa tctgtcggtg gatgacgagc catccacggt
     7021 agaagttgga ttggagggca actaatgaag acgtcgttcg atcggtttct gaatcgaaat
     7081 tttgcacttg cactcggggc ttgggcactc gttactggct gcgccagcag cgccgaagaa
     7141 actctggacc gcgatgaaac cgccagctcg acgagtgctc cgatcatcgg tggtcagaac
     7201 gcagtcgctg gccaacacaa gtggattgcc aacgtcttgg tcaacgacgg tggcgtatgg
     7261 cggcaccagt gcggcggagc actgatcgcg gcccagtggg tactgacggc tgcgcactgc
     7321 atcgcgaaca gcggtggttg gtactcgaat acgcaaattc gagtccggct tggcgactac
     7381 gatcaaaccg tctcggaggc atcggaacag ctcaagaacg tcacgacggt ctggatccgt
     7441 gacggctaca acgccgatcc gaacgtgagt ccatcgaact atgatatggc tttgatcaag
     7501 ttgagttccg cggcgacgct caacagcaac gtcaacacca tcgcgctcgc cacccagact
     7561 ccacccacgt cctgcccgcc ctcgaatctg agttgcaatg atgtcatcgc ggcaggctgg
     7621 ggcgcgaccg cgccgctgaa tccacccccg cccaccgaat cctccaacat cctgcaacga
     7681 gcgacgatca agctgcgctc tcaatcaacc tgcaacggtg cggaggtcgg agccattcgc
     7741 tcggccacgg gcagtcctgg tctcaagttc gacgagctct gcgtcggttc ttcgaacgga
     7801 aaccccgggc cttgtaaggg ggacagcggc ggaccgctgg tccgagccaa gagcggcggt
     7861 ggctacgagc tcgtcggggt gagcagttgg gggcctctct attgcacgtc gtacgccgtg
     7921 tacggcagcg tccccgccca cacggattgg attcaatggg tgttgaccca ctgaattccc
     7981 cttagggccg cgctccgagc taaagagggc cggaggtttc cgacccggcc tcttagcttc
     8041 ggagcgcgcg cctctctgga cggcggcgca tcgctcccgt cttatcgcgc acttcctcct
     8101 gctcggtaga aacaacttgg atgcgccccc aggtcggcct actctcatgg accgctgctc
     8161 gcgtctcgcc gtccgcgcag ggcctagcag cgaggttcta gtgagggagg agcaaaccaa
     8221 tgattgtgga cacgattgca acgggttcga agttgaattg gttagccaca tgctggggag
     8281 cgctattgtt agtcgcgtcc gcacctcgcg cggccgccgc gcccagctgt gacgagctat
     8341 cgatatccgc agcaagagcg ctcccgctag gctctcaggt caccatcgaa ggtaccgtca
     8401 gcacgccgtc cggcgtgttt gcctcgagct tcttcgatgc ggggttcggg attcaagatc
     8461 gtcgggcggg catttacgtc agtgtagctg ccaatcccga ggtcgttccg ggcgaccgat
     8521 tgcgagtcac gggcgtgctc gcggatagtt acgggctact cgtgctcatt cccaatggtg
     8581 ccacggacat ccaggaagtt ggcgcgggcc atccgcccgc ggctgatcga gtcgacactg
     8641 gttctatcgg cgaatcgaca gaaggagtgc tcgtagccgt caaagggagg gtcacccaag
     8701 cccccgttag cgacctccca tatggttaca aattcgaggt cgatgatggc tcgggcgagc
     8761 tccagatctt cgtcaatgta gacaccggaa tcgacctttc ttccattgcg gtcggtcgaa
     8821 agatcaaggt gaccggtctg agtagtcagt tcgaagacca ctacgagatc gatccgcgtt
     8881 tcccgcagga tgtggttctg ctcccgagaa acgatggtcc ttgagcgcga cgcgaaggag
     8941 cactcgctgt tcaatcggag gagcttcgga cgacgaccga tcacctgtcg atacagacgt
     9001 gggtccaggg cgacggcgaa ccgcgcagtt gggccgggac gcaacgttgc tctggcgggc
     9061 accctaagtc gagcccagcg cagggctgga ggcactggga cgtttgattc gagttcagag
     9121 gttggccgca tacaccaacg ccgcagtccc aagcttcagt gcaggcgctt ccgagtgcgc
     9181 cggggctagg ccgggggagc atgcacctac cctgcgtcat gtcacacgtc atcggcgccg
     9241 gacatcgaat cgttggaccg cagttcggcg cgcagtaccg cctccctggg gcatctaccg
     9301 gagaaacaca ctggccgtcc tcgcattgtg cgtcaaacca gcatcgtttt ccgggcgcag
     9361 cgcgggcttc gacctctaac gcgaattgct ggagccattc acggtgttcg tccgtcaaca
     9421 gcgttgcccc gaactcggcg tcgcattccg cgggcccgcg actcactatg gctacgagct
     9481 cgccgcgggc attgaagatg ggggcgcccg agtctccatg acagactcgc gccggcgcag
     9541 ggctcaagcg cagcagtccg gtttccggat cgatgcgctg agacatgacg gctccagaac
     9601 tacgcagaaa tggcgcttca ggtgagccgc cgaagccaac caccgtaagc acgtcgtgct
     9661 cacccggcgc ggcgtgagcc aaggatatcg gggcgaaagc cgtttcatgc gacagcaccg
     9721 cgagcacgag atcgtcctgt aaggtcaacg gatcgaagtt gcgtgtccaa cgcgcatcca
     9781 ggagcctccc gtcgttttcg atcgcgcccg ccgggctatc gaacgagaca cgcagagccg
     9841 ccgagccttg aagtaggcaa tgaccggcgg tcagaatcag ccgcgggctc acgactaccg
     9901 ccgagcacaa ctcggaccca tgatcgatca acaccgcgac gggagcagcc ccgtagttgg
     9961 cagttctccc cacccaagct gcctctgccc cgggcgtggg acgatcctcg tccgcgctac
    10021 agccgctcgc gagcgtgagc ccagcacgca cagcggaacg agcaacgccc gcgagcagcc
    10081 tcctccagca ccaaaccacg gctgagtcga gtagcgtggc tagggtttag ttgcagcggt
    10141 cgaacagtga cgccgcgtag tcatctgccg cgtcgcggaa ctgagcgccg cagtctcggt
    10201 aaaagtacgt gcagaagtcg tggtcgaagc aatcttggaa tgatccgttc ttccagctcg
    10261 actgacagcc ttctccacac tggcccatac aggacccgct ccagttgccg caacccttga
    10321 ccgagccatt gcaagactgc gtgtcgttca ccacgaagta ggcgctccgg gagtttcccc
    10381 cgtcagcctt ggcccaatac cgagtggaca caccttttcg gaggcaggtc actccatcgt
    10441 cggtaaagct ggcgtaggca acctcgccgc tgaccgttcc agacggtccg ctcgagaacg
    10501 catcgatgac cgtacctggc tgcccggcgg cgagccacga gacttgtgcg gccagcactc
    10561 ccttgtgcgt aggtaagcgg cccttggcgg tgccctctgc gtccagcgcc gcatagagct
    10621 gtttgcttaa ctccgcgaat accgtgagag attgctccgt cagaacactg ccacgcccgt
    10681 ccaccgtcac acgcccggac tggttgtcat agcgccattc aatcgtcgtt tcgccgatca
    10741 cgaccacact gacggcctga ccggtagcgt cgaattgact gtcgaagtgt gcagttcccg
    10801 cctcgctcgc aaattcaccg gtcaacgccg tgtctgaatt ttcggtgaga gatagcgctt
    10861 ccgttcccgt gcccgagcct ccgacatcct gcaccgaccc actctccaag ggcgtgcccg
    10921 gcggttcggc tgcgcaagcg acggtgagga tccacaaagc gccgactagc acagtcttac
    10981 gcggagccca cgttcgcgag cgcggagcat ctgcccgacg cggaccagta gtcgaagtcg
    11041 cgtgaagtaa actcttcatc agcaattctc cttcgggagt tagttcccgc tacaacaatg
    11101 cggggcaccc tgatttggtt cgctcttctt tacgaattgt ctgcggcggg tggcgtggcg
    11161 ctcttggcca tcg
//
LOCUS       DASTKU010000178         8237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_255468, whole genome shotgun sequence.
ACCESSION   DASTKU010000178 DASTKU010000000
VERSION     DASTKU010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255468"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFQ61_06795"
     CDS             complement(<1..626)
                     /locus_tag="VFQ61_06795"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET9954191.1"
                     /translation="MIKFDFATATEIVFGSGRARELGARAKALGTRALVVTGAHPERF
                     QSLLAAVSSAGVTLETFCVSQEPTTQTARDGVTQALAVRAELVIGLGGGSVLDAAKAI
                     AALATNGGEPLDFLEVVGRGLPLRERSLPALLLPTTSGTGSEVTRNAVLQVESERVKV
                     SLRSPWMLPTLALVDPELTLSVPPAVTRATGLDALTQVLEPFVSNARGP"
     gene            complement(628..945)
                     /locus_tag="VFQ61_06800"
     CDS             complement(628..945)
                     /locus_tag="VFQ61_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9954192.1"
                     /translation="MLTVMVKVVVHPGDVAAFAEASAENAAHSLREPGIARFDVLQSA
                     EEPTQFLLVEVYRDDEAPAKHKETAHYARWRDRVAPMMAVPRSSTKYRTVDPARVPAE
                     GTR"
     gene            complement(993..1682)
                     /locus_tag="VFQ61_06805"
     CDS             complement(993..1682)
                     /locus_tag="VFQ61_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HET9954193.1"
                     /translation="MSERRYTDAEVRAILDRALRSDLSRGVSHDQLLSVAAEVGISRE
                     ALEAAALDVEEGRETEQARDRILKRRRRAFLNHLWTFLGVQAFLIAVNLLTIPYYLWF
                     LYPLMGWGLGLFFHARYGLSKEVSEKAIQRELKRSERRRRREERDARRATGQPAGNVS
                     LGSALEQGMGLLASKVLHEIQKNVGGPDERVRVQGPGQSAGGVASGREPYENREWTEG
                     GAERAPDRQRR"
     gene            1739..2707
                     /locus_tag="VFQ61_06810"
     CDS             1739..2707
                     /locus_tag="VFQ61_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9954194.1"
                     /translation="MTSAAQRRHIGVVYSLGGALSAAVYIFPYKQAGALAPPWALAWA
                     LLLGSATMNSVSAWLIERRRSAILDASPAPAVSLWRSIEWRIAAGLALLAVTGNVASA
                     AALEHLPPAITTVLLRTELIFVTALGILLLKERPSLAFYAGALLALSGLCIMQLAPGA
                     PRSWLGVFWGLCAAASFGAMQVLTRAFIHRIRPPYVNAVRLWMATALLSFVPGAVSAA
                     RHAGLRFWGLVALAALFGPFIGRLCIMWSLRSLAAAESALLLLLSPVMAFAIGYFVFG
                     NVPRSRELLGGALMLCGIALPPLAALVIQRARAKTAARAPGTNIDS"
     gene            complement(2723..3505)
                     /locus_tag="VFQ61_06815"
     CDS             complement(2723..3505)
                     /locus_tag="VFQ61_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954195.1"
                     /translation="MLRFDLACSFQRAPLAILGLVCACSGGAPPAKSPQSATPAGGAS
                     AGVPVTATPKTNGVVLPSPESVTMQQPGGDAPDRHAAALQRLLNEQWEPRSDRDEQLR
                     IALPDAGNWKRVRYFGVEHFVGFRYGQDYHAMAVVFVLDVEEERPSSEDCLRHFDAWG
                     RPQTQPFDVDFEPFKPHHSKFQGRSLITLAVDGQLSLGFSRPKFAAAWAAYPAYAKAC
                     LISAVAVPERDAPELAKQVRDRFVAEGFAKLEPLTETRPYRK"
     gene            complement(3640..4731)
                     /locus_tag="VFQ61_06820"
     CDS             complement(3640..4731)
                     /locus_tag="VFQ61_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HET9954196.1"
                     /translation="MPKRSRSGEISRRELGRAAALVAAAAAPVLGEREAHAARSVEAL
                     TAKAPAGFSPLAIPGKISKVSSKGDFASFMQPNQLWPKAEVAKRLLERAMTDFTGAPN
                     LVEAMKKFIHPEDVVAIKVNGIAGQTGYTMAVNFELILPVVEAVMGVGVPANKITVYE
                     QFPNFLAGTRVNVREWKLPKDVKTGTHNNQDHSMPEIKVHQGIGTKYCRIFTEATAVI
                     DMTQIKDHSICGYTGTLKNITHGNINNPHKHHAGFANPQIALLYNHPIVRSRVRLHIT
                     DAFKITYDQGPLDKNPKTRIPHGSVYVATDAVAMDTMGWKIIDDERKARGLKSLKDAG
                     REPAYIRTAADLKIGVHDLSAIRMQSTEI"
     gene            complement(4833..6227)
                     /locus_tag="VFQ61_06825"
     CDS             complement(4833..6227)
                     /locus_tag="VFQ61_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET9954197.1"
                     /translation="MGSPLPGASRRFQEAVPRGRRGRMPAVMHRAALYGTLLCCVVLC
                     GALPSCRSTVDSIGYDRLAQGGAGGGELPFGGSFEAGAGGVPTPHLLPLTGPSSYPNL
                     VKDLIGKSDEEIRTKIDMLFNRLFYGNSSTESVYVLVGEDQAYIRDVLHNDVRTEGMA
                     WGMMICVQLDRREEFDRLYRYAKTLQVTTGPSRGYFRSFCDDGEGGKAQCLDPFGLQE
                     ILMSLIFAHGRWGSDGAIDYEADALALLELIRNKEAANGGVVDGVTNTFDAATFLPYD
                     LPNQSVGLTGRPSITMPAYYELWHQATADPFYSRAAQSGRKYIELAADPGTGLLPVRA
                     AFDGTPVMPGDTFQSESYRVHLNMALDRVWSGYMGDNVDLSNRILAFFAAEGLDVYCS
                     SYSLSGECLDTARDSGLMAANAMLALVSTARNRPAFVKAMWDAVLPTGSGRYFAGVLN
                     LIGLLVLSGQYRVY"
     gene            complement(6230..6967)
                     /locus_tag="VFQ61_06830"
     CDS             complement(6230..6967)
                     /locus_tag="VFQ61_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954198.1"
                     /translation="MNEPRRLSEEAESPLEEALLQAGRSYGVSSSSHRRTLMALGLAG
                     TATVSATASGLSAFGEKALQVFQLGPVKWLAAASIVAAPAAYLGVRAASTSEPTPIAA
                     PSEPSGSGDAEIAAAPVIAEAAASPSSPPVQAEEPAVSEVAPNRVPRDGRTARRATLS
                     QELQSLDSVRSELARGDVRAALGALDSYARAFPRGRLQLEAEVLRIDALARSGQQELA
                     RRRAEAFLRRAPKSVLASRVRRYVNSD"
     gene            complement(6964..7500)
                     /locus_tag="VFQ61_06835"
     CDS             complement(6964..7500)
                     /locus_tag="VFQ61_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9954199.1"
                     /translation="MRPNSADLRLRGMVDRYIDFVARVLRNAGVPAAQIDDDVQRTFI
                     AAAKRLDDVRPGAEKSFLLQVALRVAAHARRAAARRREVQSEDAPDVVEWIATPERLT
                     DQKHMRQLLDRVLGEMDADLRTVFVLYEFEEMNMAEIAGVLSIPRGTVASRLRRARAD
                     FRDRVRSLDGFSKLEVGS"
     gene            complement(7862..>8237)
                     /locus_tag="VFQ61_06840"
     CDS             complement(7862..>8237)
                     /locus_tag="VFQ61_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954200.1"
                     /translation="SHALGGQMTWVTSWVHAFLITMTIELLVGVPLLGKSRPGAAPDP
                     LWRRAGAVVFAQLASHPLVWFVLPELHLRRSNYALVAETWAVVSELVLYRLVFVDLSW
                     SRVLGVSALANGVSFAVGTFLN"
BASE COUNT         1467 a         2738 c         2531 g         1501 t
ORIGIN      
        1 ggccctcgcg cgttcgagac gaagggctcg agcacctgag tgagcgcgtc caggccggtc
       61 gcgcgggtca ccgcgggggg cacactgagc gtcagctccg gatcgacgag cgcgagcgtg
      121 ggcaacatcc atgggctacg caagctgacc ttcacacgct cggactctac ctgcagcacc
      181 gcgttgcggg tcacttccga gcccgtgccc gacgtggtgg gcagcagcaa cgcgggcaag
      241 ctccgctcgc gcagcgggag ccctcgcccc acgacctcca agaaatctag cggttcgccg
      301 ccgttggtgg cgagggccgc aatcgccttt gcggcgtcga gcacactgcc gccacccaac
      361 ccgatgacga gctccgctct caccgctaga gcctgtgtta cgccatcgcg cgccgtctgc
      421 gtggtgggtt cttgcgatac acagaacgtc tccagtgtga cccccgccga gctcactgcc
      481 gccagcaggc tctggaagcg ctcggggtgg gcgccggtca cgaccagcgc acgcgttccc
      541 aaggctttgg cccgcgctcc gagctctcgg gcgcgccccg atccgaacac gatttcggtg
      601 gcggtggcga agtcgaactt gatcaattta tcgggtccct tcggcaggaa cgcgggcggg
      661 gtctacagtg cggtacttcg tgctcgagcg cggaacggcc atcatgggcg ctacgcggtc
      721 acgccaccgt gcatagtggg cggtttcctt gtgcttcgcc ggtgcctcgt cgtcgcggta
      781 cacctcgacg agcaagaact gcgttggctc ctccgccgat tggagcacat cgaaacgagc
      841 aatgcccggc tctcgtaagc tgtgcgcggc gttctcggcg gacgcttccg cgaaggcagc
      901 cacgtcaccc ggatgcacta cgactttcac catcaccgtc aacatcgttg ctctcctcgc
      961 cgcaggaaca cggctctgtt ccggcttctt cgtcagcggc gctgccggtc aggcgcgcgt
     1021 tccgcgccac cttcggtcca ctcgcggttt tcatatggct cgcggcccga cgcaacccca
     1081 cccgcactct gccctggccc ctgtacgcgc acccgctcat ccggcccgcc aacattcttt
     1141 tggatctcgt gcagcacctt cgaggcgagc aggcccatgc cttgctcgag cgccgaaccc
     1201 aagctcacat tcccagcagg ttgtccggtg gcgcgtcgcg cgtctcgttc ctcgcgccga
     1261 cgtcggcgtt cgctacgctt gagttctcgt tgaatggcct tttccgagac ctccttcgag
     1321 agcccatagc gagcatggaa gaaaagcccc agcccccagc ccatcaatgg atacaagaac
     1381 cagagatagt acggaatcgt gagcaggtta acggcgatca agaaggcttg cacccccaag
     1441 aacgtccaca aatgattgag aaacgcgcgt cggcggcgtt tgagaatgcg gtcccgtgcc
     1501 tgctccgttt cgcgcccctc ctccacgtcg agggccgcgg cttccagagc ttcgcgcgag
     1561 atcccgactt cggcggcgac gctgagcagc tggtcgtgag acacgccgcg cgagaggtcg
     1621 ctgcgcagcg cacgatcgag gatggctcgg acctcagcgt cggtatagcg ccgttcggac
     1681 atgttcggtg gcagcagcgt aaccccgcgc ccgctcagga gccactctgg ccgcgggaat
     1741 gacctcagcc gcacaacgcc gtcacatcgg agtggtctat tcgctcggcg gcgcactttc
     1801 ggcggctgtg tacattttcc cgtacaagca agccggggcg ctggccccgc cgtgggcgtt
     1861 ggcgtgggcg ctgttgctcg gctcggcgac gatgaattcg gtgagcgctt ggctcatcga
     1921 acgtcggcgt tccgcaatcc tcgatgcctc tcccgcgccg gccgtgtcgc tctggcgcag
     1981 catcgagtgg cgcatcgcgg ccggcctagc gctactcgcc gtgacgggaa atgtggccag
     2041 cgccgcagcg ctcgaacatc taccgccggc catcacgacc gtgctgctgc gcactgaact
     2101 catcttcgtg acggcgctgg gaattttgct gctcaaagaa cgcccgagct tggcgtttta
     2161 cgcgggcgcg ctgctcgcgt tgagcgggct ttgtatcatg cagctggccc caggagcgcc
     2221 ccgctcttgg ctcggcgtgt tttggggcct ctgcgcggcc gcgagcttcg gcgcgatgca
     2281 agtgctcacg cgcgcgttca ttcatcgcat tcgtccgccg tacgtcaacg cggtccgcct
     2341 gtggatggcg acggcactgt tgagcttcgt gccgggcgcg gtttcagcgg cgcggcatgc
     2401 cggcctacgg ttttggggat tggtcgcgct ggccgcactg ttcggaccat tcatcgggcg
     2461 gctctgcatc atgtggtctt tacgcagctt ggcggccgca gaatcggcgc tgcttcttct
     2521 gctttccccg gtcatggcgt ttgcgattgg ctacttcgtg ttcggcaacg tcccgcgttc
     2581 gcgcgagctg ctggggggtg cgctgatgtt atgcgggatc gcactaccgc ccttggccgc
     2641 gctcgtgatt cagcgcgcgc gtgcaaaaac cgcggcgcga gcgcccggga ccaacatcga
     2701 ttcatgatcg ggacgggaag aattacttcc ggtagggccg cgtctcggtg agtggttcta
     2761 gttttgcaaa accctcggcg acgaagcggt ctcgcacctg cttcgcgagt tccggtgcgt
     2821 cccgctccgg aacggcgacc gcgctaatca agcatgcctt ggcgtaagcc gggtaagccg
     2881 cccacgccgc cgcaaacttc ggcctcgaaa atcccaagct cagctgccca tcgacggcca
     2941 aagtgatgag cgaacgccct tggaacttcg agtggtgagg cttgaacggc tcgaagtcca
     3001 cgtcgaacgg ttgagtctgc gggcgacccc aagcgtcgaa gtggcgcaag caatcttcgc
     3061 tcgaaggccg ctcctcttcg acatccagca cgaaaacgac ggccatggca tggtagtcct
     3121 gcccgtaccg aaaacccacg aagtgctcga cgccgaagta gcggacgcgc ttccaattgc
     3181 ctgcgtcggg cagtgcgatt cgcagctgct cgtcccggtc gctgcggggc tcccactgtt
     3241 cattcagcag ccgctgcagc gccgccgcgt gtcgatcggg cgcgtctccg ccaggctgtt
     3301 gcatcgtcac gctctccggc gacggcagca ctacgccgtt ggtcttcgga gttgcagtga
     3361 cgggcacgcc ggcactcgcg cctccggcgg gcgtagccga ctgcggggac ttcgcgggag
     3421 gtgcccctcc actgcacgcg cacaccagac ccaggatcgc cagcggggca cgctggaagg
     3481 agcaggccaa gtcgaagcgc aacatcggat gactttgcta cggagccgcg gcgcgaatta
     3541 tgccatcgac ccgctcatgc caccttctcg cgtcacaaag cagcagcggc gcccagccat
     3601 cgagcgccgc ccaatcccga gtcttctccg ggatcctgtt taaatttccg tgctttgcat
     3661 ccggatcgcg gacaaatcgt gaacgccaat cttcaaatct gccgcagtgc ggatgtaggc
     3721 gggttcccgc ccggcgtcct tcaggctctt caaacctcgc gccttgcgct cgtcgtcgat
     3781 gatcttccag cccatcgtat ccatcgcgac ggcgtcggtc gccacgtaca ccgaaccgtg
     3841 tgggatacgt gtcttcgggt tcttgtcgag cggcccctgg tcgtaagtaa tcttgaaggc
     3901 atccgtgatg tggagccgca cgcgtgaacg tacgatcgga tggttgtaga gcagcgcgat
     3961 ttgcgggttg gcaaagcccg cgtggtgctt gtgggggttg ttgatgttgc cgtgcgtgat
     4021 gttcttgagc gtgcccgtgt agccgcagat tgagtgatcc ttgatctgcg tcatgtcgat
     4081 cacggccgta gcctcggtga agatgcggca gtacttggtg ccgatgccct gatgcacctt
     4141 gatctccggc atcgagtgat cctggttgtt gtgcgtgccg gtcttgacgt ctttgggcag
     4201 cttccactcg cgtacgttga cgcgggtacc cgccaagaag ttcgggaact gctcgtacac
     4261 ggtgatcttg ttcgccggaa cgccaacccc catcacggcc tcgaccaccg gtagaatcaa
     4321 ctcgaagttg acggccatcg tgtagcccgt ttgccccgcg atgccgttca ccttgatcgc
     4381 gaccacgtct tcggggtgga tgaacttctt catcgcctcg accagattcg gggctccggt
     4441 gaagtccgtc atcgcgcgtt ccaggagccg cttggccact tctgccttcg gccaaagctg
     4501 gtttggctgc atgaaggaag cgaaatcccc tttgctggag accttgctga tcttgcctgg
     4561 aatggccaat gggctgaaac ctgcgggcgc cttggccgtc agggcttcca ccgaacgggc
     4621 ggcatgcgcc tcacgctccc ccaataccgg cgcggccgcg gcagccacga gcgcagccgc
     4681 gcggcccagc tctcggcggg aaatttcccc acttcgcgaa cgcttcggca tcgggtcaga
     4741 cataaatggc ctccgccggt ggggtatcac gcgacggaag gaaacgccaa gactcagcct
     4801 tcctgcgcga tgcccccgcg taacgcgcgc cttcagtaca cgcgatattg cccgctgagc
     4861 acgagcaaac cgatcagatt cagtacgccg gcaaaatagc ggccagagcc cgtgggtaac
     4921 acggcatccc acatggcctt gacgaaagcg gggcggttcc gcgccgtcga gacgagcgcg
     4981 agcatggcgt tggcggccat cagccccgag tcccgcgcag tatcgaggca ctcaccgctg
     5041 agtgagtagc tgctgcagta gacgtcgagt ccctccgccg caaagaacgc taggatccgg
     5101 ttgctcaagt cgacgttgtc gcccatgtac cccgaccaca cgcgatccag agccatattc
     5161 aagtgcactc ggtacgactc cgattggaac gtgtcgcccg gcatgaccgg ggttccatcg
     5221 aaggccgcac gcacgggtag caagcccgtt cctggatccg ccgcgagttc gatgtacttg
     5281 cggccgcttt gcgcggccct cgagtagaac ggatccgccg tggcttggtg ccagagctcg
     5341 tagtaggccg gcatggtgat cgacggacgc ccggtcaacc cgacgctttg attcgggaga
     5401 tcgtaaggca aaaacgtggc tgcatcgaac gtgttggtga cgccgtccac tactcccccg
     5461 ttcgcggcct ccttgttacg gatcaactcg agcagggcga gggcgtccgc ttcatagtcg
     5521 atcgctccgt cgctgcccca acgcccgtgc gcgaagatca gcgacatcaa gatctcttgc
     5581 agaccaaatg ggtccaagca ctgcgccttg ccaccctccc cgtcatcgca gaaagagcgg
     5641 aaataaccgc ggctcggccc ggtcgtgacc tgaagcgttt tggcgtagcg atagagccga
     5701 tcgaactctt cgcgacggtc gagctgcacg cagatcatca tgccccaggc cataccttcg
     5761 gtgcgaacgt cgttgtgcaa gacatcgcgg atgtacgcct ggtcttcccc gaccagcacg
     5821 taaacggact cggtcgagct gttcccataa aacagccgat tgaacagcat gtcgatcttg
     5881 gtgcgaatct cctcgtcact cttaccgatc aaatccttga ccaggttcgg atagctgctt
     5941 ggtccggtta gcggcagcaa atgcggcgtt ggaaccccgc cggctccagc ttcgaagctg
     6001 ccgccaaacg gaagctcgcc tccgccagct cccccttgcg cgagtctgtc ataaccaatc
     6061 gagtccaccg tgctgcggca gctcggaagc gcgccgcaca acacgacaca gcacagcagg
     6121 gtgccgtata gagcggcgcg atgcatcacg gccggcattc gcccccggcg gccgcgcggc
     6181 acggcttcct ggaacctacg cgatgcaccc gggagcggcg atcccatcgt cagtcactgt
     6241 tgacgtagcg ccgcacgcgc gaggcaagca cgctcttcgg agcacgccgc aggaacgctt
     6301 ccgctctgcg ccgcgccaac tcctgctgcc cgctgcgagc caacgcatcg atgcgcagca
     6361 cctcggcttc gagttgtaga cggccacgtg ggaaggcgcg cgcgtacgaa tccaaggccc
     6421 ccaacgcggc acgcacgtca cctcgagcta gctccgagcg caccgaatcg agagactgca
     6481 actcctgcga caacgtggcc cgccgagccg tgcggccgtc acgaggtacg cgattcggcg
     6541 ccacctccga gactgctggc tcttcggctt ggactggcgg tgagcttggg ctcgcggcgg
     6601 cctccgctat taccggtgca gctgcgatct ctgcgtcgcc tgagccagag ggttcggagg
     6661 gcgccgcaat cggggtgggc tccgatgtcg aagcggcgcg aactccgaga tacgccgcag
     6721 gcgcagccac gatggaggcg gcagcgagcc acttcacggg gccaagctga aaaacctgca
     6781 gcgccttttc gccaaatgcc gacaacccgc tcgcggtggc actcacggtc gcggtgcccg
     6841 ccagacccaa cgccatcagc gtgcgtcggt gcgaagagga agagacgccg tacgacctac
     6901 ctgcttggag cagcgcttct tcgagtgggc tctcggcttc ttcgctcaag cgccgaggct
     6961 cgttcacgac cccacctcga gcttcgaaaa tccatccagg gaccgaacgc gatctcgaaa
     7021 gtccgcgcgg gcccgacgta ggcgcgacgc cacggtgccc cggggaatgc tgagaacccc
     7081 tgcgatctcc gccatgttca tctcctcgaa ctcgtagagc acgaacacgg tgcgcagatc
     7141 ggcgtccatc tcacccaaga cgcggtccaa gagctggcgc atatgcttct ggtcggtcaa
     7201 ccgctccgga gtcgcgatcc actcgacgac gtctggcgca tcctccgact gcacttcgcg
     7261 acggcgggct gctgcccgcc tggcgtgtgc tgcaactcgc aaagctactt gaagcaaaaa
     7321 gctcttctcc gcgcccggac ggacatcgtc cagccgcttg gccgcggcta tgaaggttcg
     7381 ctgcacgtcg tcatcgatct gggccgccgg aacgccggca ttacgcaata cacgcgctac
     7441 gaagtcgatg taacgatcga ccataccgcg caggcgcaga tctgctgaat ttggacgcat
     7501 cgtcaacgaa gcccgggggg gtttggccag aaagcctgga aaggtagagc taccgcccct
     7561 gcacctcggc agacgacgag tccgacatgt tcgttgtccg actcatgaat gcccgaaagc
     7621 cagggaagcg atcagctgta tgagtcaacg agcgcccaga accggttcac gttgccgaaa
     7681 cggggcaaca gtggtcgggc gacgctgtgg tgatgactcg ccaaacggtc gcccctcgcg
     7741 gccgcccctc ccacaatcgg acggcgcagc aattttcgac ctatcaggcg atgcccctcg
     7801 aatgttttgg ctgagctgcc caggtactga caggccgcga gcaacccttg ggtgagccgg
     7861 ctcaattcag gaacgtgcca acggcaaaag aaacgccgtt ggctagggct gaaacgccca
     7921 gaacccgaga ccaactcagg tcgacgaaca ccagtcgata gagcaccagc tcgctcacga
     7981 ccgcccacgt ctcggcgacc aaggcgtagt tggagcgccg cagatgcagc tcgggcagca
     8041 cgaaccagac caggggatgg ctggcgagct gggcaaaaac cactgcccct gccctccgcc
     8101 acaacggatc gggtgcggcg ccggggcggg acttgccaag caggggcact ccaacgagga
     8161 gctcgatcgt catcgtgatt agaaaagcgt gtacccagct ggtgacccac gtcatctgac
     8221 cacctaaggc gtgcgac
//
LOCUS       DASTKU010000179         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1818359, whole genome shotgun sequence.
ACCESSION   DASTKU010000179 DASTKU010000000
VERSION     DASTKU010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818359"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VFQ61_06845"
     CDS             complement(<1..778)
                     /locus_tag="VFQ61_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954201.1"
                     /translation="MTLETDASQLGYQDVRDYLRAHGWTQASSKRDYVAIYRHDAPRA
                     EVLIPLDRDLADYGKAMWLAAREIATVERRTVESVLHDLRQPRLDRIRISLEGEDLEN
                     GTLALAQGVSLLSGVRKALLASASTVRHARRYHPRMSFSEAELFVQSCRLGQTEVGSY
                     VLTVEAPHRLAANAATPDQAFGRQASAILMRAVQSIVVNVRKGDAQPLLEDPSPIVSA
                     NLCDALVDMMPADEKADLRFRATWSPLLPISEGVPNQVHVD"
     gene            complement(775..1299)
                     /locus_tag="VFQ61_06850"
     CDS             complement(775..1299)
                     /locus_tag="VFQ61_06850"
                     /inference="COORDINATES: protein motif:HMM:NF025638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HET9954202.1"
                     /translation="MTGEMLWTNDIKEELSLAYVHAVVSQAGFSFETIRKDRDSVDVM
                     VRARGRICEDATLESPMLDLQLKATVMEPLPSEQFPFDVPAKNYNDLVGRTTVPRLLV
                     VYVMPERSEDWASWNEDRLVLRRSAYWTSLCGQPRTQNTSTQRIYVSRRNVFEPTSIR
                     HLLTRVAREEGFAS"
     gene            complement(1474..>2914)
                     /locus_tag="VFQ61_06855"
     CDS             complement(1474..>2914)
                     /locus_tag="VFQ61_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET9954203.1"
                     /translation="VEDLHDLAEILNAPLAQWRIFLHPSQSKLVNLKASGATRVLGSA
                     GTGKTVVAMHRARFLAKEHCPPGKKILFTTFTKNLAADIQSLMTSLCAKELEQIEVTH
                     LHGWAAALLQSRGIKVRVNPDELKKGWDDAYALDTENRFPQEFYETEWAEVVQAQGIL
                     EEAEYLRARRAGRGTRLSREQRKQVWEVLAGYRRYLQSRGLMDPADTIREARLLIEAG
                     KVPSRYAAVVADEVQDFSESELRLLRSMLPEGPNDLFLVGDAHQRIYGHKASLSRVGI
                     NVRGMRSRRLRLNYRTTQSIRNWSVAILRGMAVDDLDEGTDDALKGYHSLRQGEAPRV
                     QPFATEQEEAEFIVQTLRDWTADTEHKLSDVCLVARRNVELDNRYGPLLREAGFPVQL
                     IKTDEARLGDGIRLATMHRVKGLEFPRVLIAGVHAGNVPLLRKSYADETARRDALDSE
                     RRLLFVAATRARDELVVTGFGRRSEFLLP"
BASE COUNT          537 a          912 c          889 g          576 t
ORIGIN      
        1 ggtcgacatg aacctggttc ggaacgccct cagagatggg taacagcgga gaccacgtcg
       61 ccctgaagcg cagatctgct ttctcgtcag caggcatcat gtcgaccagg gcatcacata
      121 ggttggcgct cacgatcggg ctcggatcct cgagcagggg ttgcgcgtcg cccttgcgca
      181 cattcaccac gatcgactga accgctcgca tcaggatcgc gcttgcttga cgcccaaacg
      241 cctggtccgg tgttgctgcg ttagcggcaa ggcgatgtgg cgcctccacg gttagaacat
      301 agctacccac ttccgtttgc ccgagcctgc agctttgaac aaagagctca gcctcactga
      361 agctcatgcg agggtgatac cgccttgcgt gacggacggt ggaggcgctc gcgagcagcg
      421 ccttgcgaac cccgctcaac agggacactc cttgggccag ggccagcgtt ccgttctcga
      481 gatcctcacc ctccagcgag atccgaattc gatcaagtcg cggctggcgt agatcgtgaa
      541 gaaccgactc aaccgtccgt ctctcgaccg ttgcaatctc acgagcggcg agccacatgg
      601 ccttcccgta gtcagcgaga tcccggtcca gaggaatgag tacttcagcc cgcggggcat
      661 cgtgtcgata tatcgccacg taatctcgct ttgaggacgc ctgcgtccaa ccatgcgcgc
      721 gcaggtagtc gcgcacgtcc tgatacccaa gctgcgaggc gtctgtttcg agcgtcatga
      781 cgcgaagcct tcttcacggg ccacgcgagt gagcaggtgg cggatactcg tgggttcgaa
      841 aacgttgcgt cggctcacgt aaatgcgctg agtggacgta ttctgagtac gaggctgccc
      901 gcacaagctt gtccagtatg ccgaacggcg cagcacgagt cggtcctcat tccaactcgc
      961 ccagtcctca ctgcgctccg gcatgacgta cacgaccagc agacgtggaa ccgtcgttcg
     1021 acccacgagg tcgttgtagt tcttagccgg cacgtcgaac ggaaattgtt ccgaagggag
     1081 tggctccatc accgtcgcct tcaattgaag atccagcatc ggcgattcca aggtggcatc
     1141 ttcacaaata cgcccccggg ctcgaaccat cacgtcgacg ctgtctcggt ctttccgaat
     1201 cgtctcgaag gaaaagcctg cttgagaaac gacagcatga acgtaggcga ggctgagctc
     1261 ctccttgatg tcgttggtcc aaagcatctc gccggtcatg gtttgacctc tcgcgtactc
     1321 tctctccgat tggtggtcaa acgtcttgca tgctgccgaa ccaccagagc tccacctgcg
     1381 ccccgcgccc gcctctaaac gaaacccggg gttatcccgt gcgcgccgcc ttgggacggc
     1441 gtccgatgag cacccgcagt gttcgcggcc agttcacggc agcaaaaact cactccttcg
     1501 cccaaagccc gtaaccacga gctcatcccg ggcgcgggtg gcagctacga acaggaggcg
     1561 gcgctcggaa tcgagcgcgt cgcgccgcgc ggtctcgtcg gcgtagctct tccggagcag
     1621 cgggacgtta cccgcgtgaa cgccggcaat caggacgcgc ggaaattcga gacctttcac
     1681 gcggtgcatg gtggcgagac ggatgccatc gcccaagcgc gcttcgtcgg tcttgatgag
     1741 ttggacgggg aaccccgctt cacgcagcag cggaccgtag cggttgtcga gttcgacgtt
     1801 gcggcgggcc acgaggcaga cgtcactcag cttgtgctcg gtatcggcgg tccagtcgcg
     1861 cagtgtctga acgatgaact ccgcctcttc ctgctcggtg gcgaagggct gcacgcgagg
     1921 agcctccccc tggcggagcg aatggtatcc cttcaaagcg tcgtcggtgc cttcgtcgag
     1981 atcgtcgacg gccatgccgc gcaggatggc gacactccag ttgcggatac tttgtgtggt
     2041 tcgatagttc aggcgtaggc gccgtgaacg cattccacgc acgttgatcc ccacgcggga
     2101 caggctggct ttgtggccat agatgcgctg gtgggcgtct ccgacgagga agaggtcatt
     2161 cgggccttcg ggtaacatcg agcggagaag gcgcagctcg gattccgaga agtcttgaac
     2221 ctcgtccgct acgacggccg cgtatcgtga gggaacctta ccggcctcga tcaacaggcg
     2281 cgcctcacgg atcgtgtccg cggggtccat caagccgcgg ctttggaggt agcgtcgata
     2341 gccggccaac acttcccaga cttgcttgcg ttgctcacgg ctcagccgcg tgccgcgccc
     2401 cgcgcgccgc gcgcgtagat actcggcctc ttccaggatg ccttgtgcct ggaccacctc
     2461 cgcccactcg gtctcgtaaa actcctgcgg gaaacggttc tcggtgtcga gcgcgtaggc
     2521 gtcgtcccaa cccttcttga gctcgtcagg attcacacgg accttgatgc cacggctctg
     2581 aagcagggca gcggcccagc cgtgcagatg agtgacctcg atttgctcca gctctttcgc
     2641 gcaaagcgag gtcattaggg actggatatc tgcggcgagg ttcttggtga aggtggtaaa
     2701 gaggatcttc ttgcccggcg ggcagtgctc ttttgcgaga aatcgcgcgc ggtgcatggc
     2761 cacgacggtc ttgccggttc cggcgctacc cagcacgcgc gtggcgccgc tggccttcaa
     2821 gttcaccagt ttgctctggc tcgggtgcag gaagatgcgc cattgggcca agggagcgtt
     2881 taggatctcc gccaaatcgt ggaggtcttc caca
//
LOCUS       DASTKU010000180         8009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1595854, whole genome shotgun sequence.
ACCESSION   DASTKU010000180 DASTKU010000000
VERSION     DASTKU010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595854"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFQ61_06860"
     CDS             <1..618
                     /locus_tag="VFQ61_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954204.1"
                     /translation="THYNWRAPVEPGSTKLTSPLTPFSPGTLRVFGPADVTEIELAPL
                     NSGIQWVTPGHDFFVKTALAVGDREPCDRLPLTFKTDTPAVCSGPEGELTWPMSRSTD
                     GAVHAQREGVCKLRVSADGARYFGPLSLSLFDLNAPEGLVLPVVGEVCSGEGATTCEW
                     GRDGTLVCASGKWKRANRCTATQACDLADGCLDGIDCGACRDLRP"
     gene            674..1012
                     /locus_tag="VFQ61_06865"
     CDS             674..1012
                     /locus_tag="VFQ61_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954205.1"
                     /translation="MWSMVEGGNTLGTAGSEGGKILIDEEHELGARITLEEGGSVAPF
                     AITCGIYGSMVHTRFFGSREAGEKEVAAMKDALHLILTSFPDADDPERERKMEGVYDA
                     VSRFVEEFPS"
     gene            complement(1496..2419)
                     /locus_tag="VFQ61_06870"
     CDS             complement(1496..2419)
                     /locus_tag="VFQ61_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPTI/Kunitz domain-containing protein"
                     /protein_id="HET9954206.1"
                     /translation="MSQLTRLLSAFPVLVAFTACGGSANHEASGGSSNNTGGAPFGGS
                     SATAGGRIGAGGAYSVGGSTAGGAMSTFGGAGGVTSAGAGGGHDPSVCSLPADAGPCD
                     AAIPSFFHNPASHQCEPFVYGGCQGNANRFNSIAECESACPGATPSIDACDTSLDCLV
                     QSAGCCGACEPVTAGDLIAINRTHVNDRMCTVSCGPCPELPRETTSRYFIAGCAAHRC
                     QVVDIRETQAVACKVDSDCYLRAGAGCCPICTSPPVALSVDANVADLFCGGLPLPCPA
                     CAPAIPPGYSTTCEGGKCVVHEPRCTGSAPC"
     gene            2815..3720
                     /locus_tag="VFQ61_06875"
     CDS             2815..3720
                     /locus_tag="VFQ61_06875"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET9954207.1"
                     /translation="MPNPGRPADERIWWLKATPFFLVHLAPLGLLWTGLSARNAVLCV
                     GLYAARMFFITAGFHRYFAHRAYKMGRVMQFVMAWGASTSCQKGVLWWAAHHRAHHRY
                     SDTERDIHSPKRGLWWSHVGWILCRKYQATDFGAIRDFTKYPELMWLNRYHLVPGIVL
                     GAACYAWGGLPSLLAFFLSTVLLYHGTFSINSLMHLWGRRRYVTTDTSRNSFLLALVT
                     LGEGWHNNHHYYQSTANQGFFWWEVDISYYVLRLLGLVGLVRDLRTPPPHVLTRNLVK
                     DAASDAPAVSPSVKAEGFAVTAGGE"
     gene            3724..4659
                     /locus_tag="VFQ61_06880"
     CDS             3724..4659
                     /locus_tag="VFQ61_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET9954208.1"
                     /translation="MSPRRPEDYRTLFWMFVLTPALVVLHFLRPDLGVWLLPLSCYFA
                     IATGVIAHNPMHRPVLHGKTANEVFSGWLSIFYGYPVFAWLPTHNLNHHKFVNRRGDA
                     TITWRYTNRHNVFVAATYFFVSAYFQSVWIKDYIRDARRNKPEAFRRIVGQYLVTYGA
                     HAGALALAVSQRGPLPGLKLWACSLGIPAFAALWTIMLFNYCQHVHTDPWSEHNHSRN
                     FEGKLLNFLLFGNGYHTVHHEKPSLHWADAAREHAAIRPLIDSRLCHRSMWWFFFQQY
                     VLAPFWPSLGTSQVGRAPFDDGSEAPVAESLPLSA"
     gene            complement(4868..6136)
                     /locus_tag="VFQ61_06885"
     CDS             complement(4868..6136)
                     /locus_tag="VFQ61_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954209.1"
                     /translation="MMTSRVLPIVLGWIACTPVHDFDEVTRETGRVASGGQGGQIQQS
                     QSGGYPAASGGVSTSGGSASSGGTSQVAGAGAGFGGAQGFGGGENSSGGSERFGQGGQ
                     TAAGAANGGAGSGGGGMGANGGANTGGVTSSSGGTSRGGGTSRGGGTGAGGSMGTGGK
                     APNGGVAGALPIDLPNTPVTGTFTVSQEASWEVNGTFLPTFEIHTPSASYWLVKSLGM
                     LVSITDGTGSDARQWIDFSTGFRPLRGLPSYGTFGTTENMTTTLDADSQTPTHLRLLS
                     VSKSGSWRLVWDFYPTHVTLTVNSAPTPYGIAYRGVPGGLLDASDRFVVSDGTSQSAT
                     VSAVTDWAGPAEWVYATDPTLKRSLFAIHHGDDSLTDRYQVKDNDSAMISFGDGAMTE
                     LPQRFSFGIVPSSDHAVVKARAQFVIDAIR"
     gene            complement(6143..7024)
                     /locus_tag="VFQ61_06890"
     CDS             complement(6143..7024)
                     /locus_tag="VFQ61_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954210.1"
                     /translation="MKSPTRSLSFRLQTVGAAIILAALFSAEPGRAASDEQATQCLDA
                     YESGQRSRKVGALREAKASFALCSSEACPRALHVDCSRWLDEVEQAIPTGVFHVTGEG
                     GRELDSVEVAIDGGSKQELDGRAITFDPGEHTVVFSRSGYESHEQRFTFSEGDKLARH
                     DVSLVPSAPASTRPTPVAEPKATPQRASLLPAWVGASVGVVGAAGFAWLGSTARRDDN
                     ALDGCSPRCSTARVDDVKREYLLANVSLGVGAAGFLAAGAWLLFRPNESPAKDTGRAS
                     PRVEVRIGAVTSLTCRF"
     gene            complement(7021..>8009)
                     /locus_tag="VFQ61_06895"
     CDS             complement(7021..>8009)
                     /locus_tag="VFQ61_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954211.1"
                     /translation="RRTDVFAAGVVLWEVLTGERLFRGAGVTETAALMNILEKRVEPP
                     SKYRSGVSAELDRVVLRALERDPARRFGSARDFALSLEQAVDAATSSDVAGSVLDLCA
                     QRLARGAATLERFREGLGRIGSSADSELDSSAVPTRLNPVVVVPSAGLLSPDLGTPLP
                     PHASDDAQTVDVQGVLVDESTRVSALSPRSARFAFALISLAVALLVGRAVLAPHASVA
                     PVPPQAADPPRTLPPQPSPAKSTIVALTPASRSEEPEAHPPPLDLSMLPPLRQRPEVR
                     RSPRPSDATKRPAAVPARSERPRPPVSKAADPCSPPTYTDADGIRHYKRECL"
BASE COUNT         1366 a         2569 c         2637 g         1437 t
ORIGIN      
        1 actcattaca actggcgtgc gcccgtcgag ccaggctcga ccaagctgac ttcgccgctg
       61 acgccattct ctcccggcac gctgcgtgtg ttcggccccg cggacgtcac cgagatcgag
      121 ctcgcgccgc tgaactcggg gattcaatgg gtcacgccag gtcacgattt tttcgtaaag
      181 accgcccttg ccgtcggcga tcgcgagccc tgcgatcggc ttccgctcac gttcaagacc
      241 gatactcccg cagtttgctc ggggcctgag ggtgagctca cgtggcccat gagccgcagt
      301 accgacggcg ccgtccacgc gcagagagaa ggggtttgca agttgcgcgt ttcggctgat
      361 ggcgcgcgct acttcggtcc cttgtcgctg tctctcttcg acttgaatgc accggagggc
      421 ttggtgttgc ccgtcgttgg cgaggtctgt agcggcgaag gcgcgaccac ttgcgaatgg
      481 ggacgcgatg gcacgctcgt ttgcgcgagc gggaagtgga agcgagcaaa tcgctgcaca
      541 gccacgcaag cctgcgacct cgccgacggc tgcctcgacg gcatcgactg cggcgcgtgc
      601 cgtgaccttc gtccgtgagc gcggcgtggt ttggccggag cggtgtaacg tatagtgcgc
      661 gagaggacgc acgatgtggt cgatggtcga aggcggtaac acgttaggaa cagcgggttc
      721 cgaaggtggc aagatcttga ttgacgagga gcacgagctc ggagcgcgca taactttgga
      781 agagggcggg agcgtcgcgc cgttcgcgat cacgtgcggc atttacggga gcatggttca
      841 cacgcgcttt ttcggctcgc gcgaagcggg tgagaaagag gttgcggcga tgaaagacgc
      901 tctccacctc atcttgacga gttttcccga tgcggacgat cccgagcgcg aacggaaaat
      961 ggaaggtgtc tacgacgccg tttcgcgctt cgtggaggag tttccgtcgt agggccccca
     1021 tggatgggtc atgctgagcg tgcgacttct tactgcggac ggaccccatg ccgcgctcgc
     1081 ggctcgcttg cgagtgaccc gtcatggtcc accaggtgag caaggtcccc aaggtcctgc
     1141 agggctcttg gagtctcagg attcatcgga tctccgaacc acgcatcggc ccgccgcttt
     1201 ccggatttct ggggccccga aaaccacaca aaccaaagta taggaacgaa ctctttcgaa
     1261 gcacgggtcg gaaccaactc tttcgtgccg agccctctgc gagcgaacga gacgaaggcc
     1321 tacctgctcg cgacatgtga cgaatcctga gcttggagta gggcagcgag cacggaccga
     1381 cgtcatgcgc gtgcggcggg ggccaccaaa acaagcgact aggtcacgcc gcgatggctt
     1441 cctagcgagc gagtcggccg gattcgccgc atcgaacggc cgccggaacc gccgctcaac
     1501 agggcgcgct gccggtgcat cgaggttcgt ggacgacgca cttcccgccc tcgcacgtgg
     1561 tcgagtagcc cggcgggatc gcgggggcgc acgcagggca ggggaggggc aagccgccac
     1621 aaaacaggtc ggcgacgttt gcatcgacgc tgagcgcgac gggcgggctc gtgcagattg
     1681 gacagcagcc tgctccggct ctcaagtagc agtcgctgtc cactttgcag gcgacggcct
     1741 gagtctcccg aatgtcgacg acttggcagc gatgcgccgc gcagcctgcg atgaagtagc
     1801 ggctcgtggt ctcgcgcggc agctcggggc acggcccgca actgaccgtg cacattcggt
     1861 cgttcacgtg ggttcggttg atggcgatga gatcgccggc ggtcactggt tcacacgccc
     1921 cgcaacaacc cgcgctctga acgaggcaat cgagtgaagt gtcgcacgcg tcgatgctcg
     1981 gagtagcacc ggggcacgcc gactcgcatt cggcgatgct gttgaagcga ttggcgttgc
     2041 cctggcaacc tccgtaaacg aagggctcgc actggtggct tgccggattg tgaaagaagc
     2101 tggggatggc cgcgtcgcaa ggcccggcgt ccgccggcag tgagcagacg ctcgggtcat
     2161 gtccaccgcc agcgccggca ctggtgacgc caccggcgcc accgaacgtg gacatcgcgc
     2221 cgcccgcagt cgagccaccg acgctgtatg ccccgccagc gccgatgcgt ccgccggcgg
     2281 tcgcgctcga gccgccgaac ggcgcgcccc cggtgttgtt cgagctccca cccgaggcct
     2341 cgtgattcgc gctgccgccg cacgccgtga acgcgaccaa gaccggaaag gctgaaagca
     2401 accgcgttag ctggctcatt acgtcgggga acttcaagtc tcatgccaga cgagggtccc
     2461 ccgttcgtcg gggttttcgc gggagcctgt gccacgggtg tgccagctca gctgctcccc
     2521 gggaaaggaa tgccgccgag ggcgccgtgt cgaccccaga gggcgtcgtg tcgaccccag
     2581 agggcgtcgt gtcgacctag aactgcgcag tgcagctgcg gctgagcggc agtgagcacc
     2641 ggtccatgga agcagttcga gccacgcgtg gcgcatcgat tgcgcggcga tgcgcgaaac
     2701 cttgcgtgtc gcgaaagtct tgcgcgaagg agcgtgattt gcgcgcattc gtcatattcg
     2761 ttgtgaccaa atgctgacga gagagcgaac tcagcggagt tagtagtcgg cagcatgccg
     2821 aacccgggac gacccgctga cgaacgcatt tggtggttga aggcaacgcc cttctttctg
     2881 gtgcacctgg ccccgctggg cctcctgtgg actggtctca gcgcgcgcaa cgcagtgctt
     2941 tgcgttggcc tttatgcggc gcggatgttc ttcattaccg cgggctttca tcgctatttt
     3001 gcgcatcgcg cctacaagat ggggcgagtg atgcaattcg tcatggcctg gggagcctcc
     3061 acctcctgtc aaaagggtgt gttgtggtgg gcggctcacc accgcgcgca ccatcggtac
     3121 tctgacacgg agcgcgacat tcactcgccc aagcgtggcc tgtggtggag ccacgtgggg
     3181 tggatattgt gccggaagta tcaagccacg gacttcggcg cgattcgcga ctttacgaaa
     3241 tacccggagc tcatgtggct caatcgctat cacttggtgc caggcatcgt cctcggcgct
     3301 gcctgctatg cctggggcgg cctgccgtca ctgctcgcgt tcttcctgtc gaccgtgttg
     3361 ctctaccacg gcaccttcag catcaattcg ttgatgcacc tctgggggcg gcgccgctac
     3421 gtgaccacgg acacgagccg gaactcgttc ttgctcgcgc tcgtgacgct cggcgagggc
     3481 tggcacaata accatcacta ctatcaatcg acggcgaacc agggcttctt ctggtgggaa
     3541 gtcgacatca gctactacgt tctcaggctg ctcggcttgg tggggctcgt gcgcgacttg
     3601 cgcacgccgc cgcctcacgt actcactcgc aatttggtga aggacgcggc ctccgacgct
     3661 ccggccgtga gccccagtgt caaggcagag ggcttcgccg tcacggcggg gggagagtaa
     3721 gccgtgagcc cgcgtcgacc cgaagactat cgaaccttgt tctggatgtt cgtgctgaca
     3781 ccggcgttgg tcgtcttgca cttcctgcga ccggatctcg gcgtgtggct gctcccgctg
     3841 agctgctatt tcgcgatcgc cacgggggtc atcgcgcaca accccatgca tcggccggtg
     3901 ctgcatggca agaccgccaa cgaggtattc tcggggtggc tctcgatctt ttacggctac
     3961 ccagtgttcg catggctacc cactcacaac ttgaaccacc acaagttcgt gaaccggcgc
     4021 ggcgatgcga cgattacttg gcgttacacg aatcgacaca acgtcttcgt ggccgccacc
     4081 tacttcttcg tgtcggctta ctttcagtcg gtttggatca aggactacat tcgagacgct
     4141 cgccgtaaca agccagaggc gtttcggcgc atcgtaggtc agtatctggt cacttatggc
     4201 gcgcacgcgg gggccctcgc gctggcggtg agtcaacgcg gcccgctgcc ggggctcaag
     4261 ctttgggcgt gctccctggg catcccagcc tttgcggcgc tttggaccat catgttgttc
     4321 aactactgcc aacacgtgca caccgacccg tggtcggagc acaatcactc gcgcaacttc
     4381 gaaggtaagc tcctcaactt cttgctgttt ggtaacggtt accacaccgt gcatcacgag
     4441 aagccgagcc tgcactgggc ggatgccgct cgcgagcacg ctgcgattcg ccccctcatc
     4501 gactcgcgcc tgtgtcatcg aagcatgtgg tggttcttct ttcagcagta cgtgctcgcg
     4561 ccgttttggc ctagcctcgg caccagtcag gtagggcgtg ccccgttcga cgacgggagc
     4621 gaagcgccgg tcgccgagag cctgccgctt tccgcatagt ctcgggttgg ttgcggtgag
     4681 cacgcgcggg cgactcgctc agtccgcgcc ttcgcccacc ttgtagtgtg ccagctgcgc
     4741 tcgcgtgcgc tggtccgcgg cgaaagtgtc gccggaccaa ctcgtggcgc gggaaaggcg
     4801 cacggatgca gcaccagaag ttcgcgtgcg tagagctgcc tcgagcctca cccgaccgcc
     4861 atcgaagtca gcggatcgcg tcgatgacga actgggcgcg agccttgacc accgcgtggt
     4921 cagagctcgg gacgatgccg aaactgaaac gttgaggcag ctccgtcatc gcgccatcgc
     4981 cgaaggaaat catggcgctg tcgttgtctt tgacttggta gcggtcagtg aggctgtcgt
     5041 cgccgtggtg aatcgcgaag agcgagcgct tcagcgtcgg gtcggtcgcg tacacccatt
     5101 cggcagggcc cgcccaatcc gtgaccgcgg aaaccgtggc cgattgggac gtgccatcgg
     5161 agacgacgaa gcgatcgctc gcgtcgagga ggcctcccgg cacgcctcgg taggctattc
     5221 cgtacggcgt cggcgcgctg ttcaccgtca gcgtcacgtg cgtcgggtag aaatcccaaa
     5281 ccagccgcca acttcctgat ttggacactg acagcagtcg caagtgagtg ggcgtctggc
     5341 tatccgcgtc gagcgtcgta gtcatgttct cggtcgtgcc gaaggtgccg taactaggca
     5401 aaccacggag cggccggaag cccgtgctga agtcgatcca ttgccgagca tcgctgccgg
     5461 ttccatcggt tatcgatacg agcatgccga gcgacttgac gagccaatag ctcgcgctcg
     5521 gcgtgtgaat ttcaaaagta ggtaggaagg tcccgtttac ttcccagctg gcctcttggg
     5581 agacggtaaa ggttccggtc accggagtgt tcggcaggtc gatcgggagc gcgcccgcga
     5641 ctccgccgtt cggcgctttg ccgccggtgc ccatcgagcc gccggcgcca gtgccgccgc
     5701 cgcgcgaggt gccgccgccg cgcgaggtac cgccgctgct cgatgtgacg ccgcccgtgt
     5761 tggcgcctcc gttggccccc atgccgccgc cgccggagcc cgcgcccccg ttcgcggcgc
     5821 ccgcggcggt ttggccgccc tgaccaaagc gctcgctccc gccgctgctg ttctctccgc
     5881 ccccaaaccc ttgtgcgcca ccgaacccgg cgcctgcgcc cgcgacctgc gaggtgcccc
     5941 ccgacgaggc actaccgccc gacgtgctca cgcctccgga cgccgcggga tagccgccgc
     6001 tttgcgactg ttgaatctgc ccgccttggc cgccgcttgc gactcggccc gtctcgcgcg
     6061 tcacttcgtc gaaatcgtgg acgggagtac acgcgatcca acccagcacg attggcagaa
     6121 ctcggctcgt catcatggtg cttcagaacc tgcaagtcag cgaggtgacg gccccaatgc
     6181 ggacttccac tcggggggag gcacgtccag tgtccttggc tggcgactca ttcgggcgaa
     6241 acagcagcca cgcgcccgct gccaagaacc cagctgcccc gacgccgagg gagacgttgg
     6301 caagcaggta ctcgcgcttg acgtcatcca cgcgtgcggt gctgcaacgc ggcgagcagc
     6361 cgtcgagggc gttgtcgtca cgtcgcgcgg tcgagccgag ccaagcgaac ccagctgccc
     6421 cgaccacgcc gacggaggca ccaacccagg cgggcagcag ggatgcccgt tgcggcgtgg
     6481 cctttggctc cgccactgga gtcggccgcg tcgacgcggg tgcggagggc acgagtgaaa
     6541 cgtcgtgacg ggccagcttg tccccttccg agaacgtgaa gcgttgttcg tgggactcgt
     6601 accccgagcg ggagaagacg acggtgtgct ccccggggtc gaaggtgatt gcgcgaccgt
     6661 cgagctcttg cttcgagccg ccgtcaatgg cgacctcgac cgaatcgagc tcacggccgc
     6721 cctccccggt gacgtgaaat acgccggtcg ggatcgcttg ttcgacttcg tcgagccagc
     6781 gggagcagtc gacgtgaagc gctcgcgggc atgcttcgct gctgcagaga gcaaaagagg
     6841 ctttcgcttc gcgtagcgct ccgactttgc gcgagcgctg tcccgactca taggcgtcga
     6901 ggcactgcgt ggcctgctcg tcggaggcag cgcggccagg ctccgcgctg aacaacgcgg
     6961 ccaagatgat ggcagcgcca accgtttgga ggcgaaacga gagactccgc gtcgggctct
     7021 tcatagacac tctcgcttgt agtgacgaat accgtccgca tccgtgtagg tgggtggaga
     7081 gcaagggtcc gcggctttcg aaaccggagg acgcggacgt tcgctgcgag cggggacggc
     7141 agccggccgc ttcgttgcgt cggagggccg cggactgcgt cgcacttcgg gacgctggcg
     7201 cagcgggggg agcatcgaca gatcgagagg cggagggtgg gcctccggct cctcgctgcg
     7261 cgacgcagga gtgagtgcga caatggtgct cttcgccggg ctcggttgcg gcggcaacgt
     7321 tcgtggtgga tcggcagctt ggggcggcac cggggctacg ctggcatgcg gtgccagaac
     7381 cgcgcgcccg acgagaagcg cgacggccaa gctaatcagc gcgaaagcaa accgcgccga
     7441 gcgaggagac agcgcggaaa cccgggtcga ctcgtcgacc aggacgccct gcacgtcgac
     7501 tgtttgggcg tcatccgaag cgtgcggcgg gagtggcgtg ccgaggtccg gcgacaacaa
     7561 cccggcggat ggcacgacta ccactgggtt caagcgagtc ggcacggcag agctatccaa
     7621 ctcggagtca gccgagcttc ctatacgccc gaggccctcg cgaaatcgct cgagagtcgc
     7681 ggcgccgcgc gcgagtcgct gcgcgcacaa gtcgagcacg ctcccggcga cgtcggagct
     7741 cgtggccgca tccactgctt gctccagact caacgcaaaa tcacgagcac tgccgaaccg
     7801 tcgcgccgga tcgcgctcga gggcacgcag caccacccga tcgagctcgg cgctgacccc
     7861 ggatcgatac ttgctcggcg gctcgacccg cttctcgaga atgttcatga gcgcggccgt
     7921 ctcggtcact cccgctccgc ggaaaagacg ttctccggtg agcacctccc acaacacgac
     7981 gccggccgcg aagacgtcgg tgcggcggt
//
LOCUS       DASTKU010000181         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_255733, whole genome shotgun sequence.
ACCESSION   DASTKU010000181 DASTKU010000000
VERSION     DASTKU010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255733"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFQ61_06900"
     CDS             complement(<1..546)
                     /locus_tag="VFQ61_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954212.1"
                     /translation="MPSLLHEGLIALVRDKPEFAADLLREVLHVDVPKFTEARLAEAS
                     LTELVPTEYHADAVVLLVDGKPVFGIIIEAQLQDDARKRFTWPVYVVTARARFECPFV
                     LVVVTPDAATARWASQTVELGAGFSWAPLVIGPEGVPIITDAERARREPELAVLSVMA
                     HGKGDTQTAVSIALAAAASLDN"
     gene            2112..2366
                     /locus_tag="VFQ61_06905"
     CDS             2112..2366
                     /locus_tag="VFQ61_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954213.1"
                     /translation="MHAHKIRVENGRIKLDEPTDLPNGTELVVVPIDDVGDLVLMQDD
                     GLEDEERSELLQSIDEGLADVDAGRVHDFKEVLATLGKRE"
     gene            2363..2659
                     /locus_tag="VFQ61_06910"
     CDS             2363..2659
                     /locus_tag="VFQ61_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954214.1"
                     /translation="MKIVVTVRARREIERIDAWWRANRDHQDLFKEELAEAEDFLAST
                     PELARVYVIRGKRKVRWLLLRKTEVKLYFWLDEQHAVVRIVSAWGGRRGHTPKV"
     gene            2713..>2903
                     /locus_tag="VFQ61_06915"
     CDS             2713..>2903
                     /locus_tag="VFQ61_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954215.1"
                     /translation="MRVPRGCGLQDRAGPDVPGGEQVIEYTLFDLPKKIITGPGATQS
                     DHSLRISSNGATVEERFAL"
BASE COUNT          559 a          928 c          942 g          474 t
ORIGIN      
        1 gttgtcgagc gaggccgccg cggcgagagc gatagagacc gcggtttgcg tgtcgccttt
       61 gccgtgggcc atgacagata ggaccgcgag ctcgggctcg cgccgcgccc gctcggcatc
      121 cgtgatgatg ggtacgcctt cgggcccgat gacgagcggc gcccaggaga aaccggcacc
      181 gagctcgacg gtttgcgatg cccatcgcgc ggtggcggca tcgggagtga cgacgacgag
      241 cacgaacggg cactcgaagc gcgctcgggc ggtcacgaca tataccggcc aggtaaagcg
      301 cttgcgcgcg tcatcttgga gctgcgcttc aatgatgatg ccgaagacgg gcttgccatc
      361 gacgagcagc acgacggcat cggcgtggta ctcggtggga acgagctcgg tgagcgaggc
      421 ctccgcgagc cgagcctcag tgaacttcgg cacgtcgacg tggagcacct cacgcagcaa
      481 gtccgctgcg aactccggct tgtcgcgaac gagtgcgatg agtccttcgt gcaagagcga
      541 cggcatcggg gggcgacgat agcgcagtga gcgtgagccg cgcgaagaag ttgttcagag
      601 agcggacgaa caacgacgcg ggtgatgccg cgaagaggcc cgcgtgggag ggacccacgc
      661 ggggcgggcg ggagccgcac agcgagaaac agtgacgaag cctctaacag cgcgttgagc
      721 agcggcgggg cgttacagca ccgcaccgag ccagtgccgc tcgctcgagc acaggcgagc
      781 cgtttacagg gccgcgctgg ccaccggctg ctccaacgct ccgttagacg caagcgacgt
      841 aaaatgcggc gcgcagggcc gtttctgtcg cccgatttct ccgtttatcc gcccagcgag
      901 gacgcagcta cactcggcgg cgtgactgga ctttgcgatg cccctgcgaa ccacgcgccc
      961 agcgctgcgt cgttgcgcgg agtgcagacg cgtcgcggcg gtcgcgtcac ccgctctgcg
     1021 tacgtccggc cacgagcctg tgcggaccaa aacacgcgcc ggggatttcg actccgagcg
     1081 cgtggcgcgc atcagcgttt tcgagcagct aaagagagaa atcgcttcga gaaaagcgct
     1141 gcgcttacgg cgaacacgca tctgctaacc ctctggggca ggagggaggt cccgatagac
     1201 ggctggcgga caactctgta ccgctaccgg aaagtccgct cggggagctg attgtcacag
     1261 gggacccgcc cgagccgagc ttgccgccgg ttccgcggct tgagcttaca cgcccgtccc
     1321 tccccatcga tgccgggcac cagcccttca gtcgagcact gttgagtcac tatctgcgcc
     1381 ggatacttgg gcttcccctt cctatctcgc agtcgctgct gacggtcggc gtgcaatcgt
     1441 ctcccccgga gagtctgctg gaatgcccta gagtgaacac tccactgcct tcggcgggcc
     1501 gcttctgcac acgttgccga gcagtactgc tgacctcgct cgcacccggg agcagaccat
     1561 gcagactcga tggcagccgg cacagcgcag aaaccgccag acctcttcgg gaacgccagc
     1621 gcacactgcc ctcccgaaaa tcaggtccta tttctggacg aaaccgaacg aacctgcgat
     1681 acgcccctat ggcgcgtcgc acactgcgcg atcgccagga aggctcgggg tgccacccgg
     1741 gccttcaacc ttattctcgg gagctgccag cctacgagcc gaagctcgtc ccggccaagt
     1801 ttcctcctgc cgactctccg cgcccaagcc ggtgaagcaa tctggacgcc tcccgcgctc
     1861 ttcaccggaa ttacgcggaa agtgatgccc gtcgacatag acgtggctac cgcgtcccag
     1921 tcgagcgcgt agagctccca gaacgtggcg tcgcagctaa agcgcgccgt tcgcgagccg
     1981 gctcccgcgc gcgactcaca ccgcggcgca aaccgggctg gcacgtcccg aagcccccgg
     2041 gagccttcat gcttcgacac ccgacgagat acggcgcggc gaaagtggcg taaagatgct
     2101 agcttggcct gatgcacgcg cacaagatcc gagtcgagaa cggccgcatc aagctcgatg
     2161 agccgacgga cttgccgaac ggcaccgaac tcgtcgttgt gcctattgat gacgtcggtg
     2221 atttagtgct catgcaagat gatgggctcg aagatgaaga gcgaagcgag ctcttgcaaa
     2281 gcatcgatga ggggctcgcg gacgtcgacg ctgggcgcgt gcacgatttc aaagaggtgc
     2341 tcgctacgct cggcaagcga gagtgaagat cgtcgtcacc gttcgcgctc gtcgcgagat
     2401 cgaacgcatc gatgcttggt ggcgagcaaa ccgcgatcat caggacctct ttaaagaaga
     2461 gttggccgaa gccgaggact tcctcgcaag cactcctgag ctcgcgagag tgtacgtcat
     2521 ccgtggcaaa cgaaaagtgc gctggttgct cctgcgcaag accgaggtga agctgtactt
     2581 ctggctcgac gagcagcatg cggttgttcg gatcgtctct gcgtggggtg ggcgccgcgg
     2641 ccacaccccg aaggtgtagc gcagcggagc gaacacgccc gcaaggctcc cttcgccgct
     2701 cgcttcgggc gtttgagggt tcctcgcggg tgtggactac aagatcgagc gggcccggac
     2761 gtccccggcg gggaacaggt gattgagtac accttgttcg acttgccgaa gaagatcatc
     2821 accggccccg gcgcaaccca gtcagaccac tcgcttcgca tcagctccaa cggcgccacc
     2881 gtcgaggagc ggtttgcgct ata
//
LOCUS       DASTKU010000182        11897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_702829, whole genome shotgun sequence.
ACCESSION   DASTKU010000182 DASTKU010000000
VERSION     DASTKU010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11897
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702829"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..765
                     /locus_tag="VFQ61_06920"
     CDS             154..765
                     /locus_tag="VFQ61_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9954216.1"
                     /translation="MARPRTRSLPQKESYHHGDLRHALLESALAIVSEEGVHDLSLRE
                     VARRAGVTYAAPYHHFADKSALLAAVGAQGFEVLIRELERASSRSEDLRGQLLAMAEA
                     YLAFARSHPSHYRVMFLPELKITDVEEYHRAGDRAFDMLVSRVKLARPGDPETAQLTV
                     AATVWAALHGLSLLSIDGTLEHKFAKINKMLRQACGAIVRMVL"
     gene            882..1172
                     /locus_tag="VFQ61_06925"
     CDS             882..1172
                     /locus_tag="VFQ61_06925"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9954217.1"
                     /translation="MFVIELIYKVPLAEIDACMNDHVAFLKRHYDSGNFLISGRKIPR
                     DGGVILALGASREAVEAIIREDPFVSRGLSDYRIIEFRASFTADDMPKRVRG"
     gene            1391..2002
                     /locus_tag="VFQ61_06930"
     CDS             1391..2002
                     /locus_tag="VFQ61_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954218.1"
                     /translation="MDCSSLSPAQLIEILCNRRRTAELVATLEPVVRTRVTRTLCRFG
                     RCGHEEIEDATQEVLIELFTKDARTLRSWQPERGLSLLGFVQFVAHRVALARLKQVWL
                     DRAQQPLEACEDRAADSVLRPDRKLATRRQFERLLERLQVEVSPRGVQLFRALFMEQH
                     SLSRVCSEFGLQPDAVYAWRSRLSRRARNICAELEPPSTPRTA"
     gene            complement(2211..3422)
                     /locus_tag="VFQ61_06935"
     CDS             complement(2211..3422)
                     /locus_tag="VFQ61_06935"
                     /inference="COORDINATES: protein motif:HMM:NF042890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6748 domain-containing protein"
                     /protein_id="HET9954219.1"
                     /translation="MSKNWNLLFSAAACWLVAACSSTAPEMSGAEDLGAASGDSDSAQ
                     QALAKAPVASYFTLRPDLRRCAFPRCGGYFVKAVNQKLTRCVDGTSQAECYVADLDLS
                     ALALDADSEQVVRGSSTEFLLRGAIGPKDFVSLGTFGTLRVLDAYHGHPGVTPSGSFF
                     QANETGIVCITFPCPSFKAQLLNSSTAPASVAQIDLSGVSSDPSDGFAQLHEPEGLLL
                     AASRKTVTGPAGQGFALIGSEYYLPFKGKGPAACGSRGLSECAAGEYCDFPPGANCGR
                     ADAPGVCASKPQICTKEFKPVCGCDGVTYSNACQAAASGVSVEFDGPCAPEPKVCGTI
                     VGLSCDEGEYCDFGVGQCLTADAGGECRPFPQVCLEIFDPVCGCDGKTYSNSCFAQMV
                     GAQIDHAGSCK"
     gene            complement(3650..4102)
                     /locus_tag="VFQ61_06940"
     CDS             complement(3650..4102)
                     /locus_tag="VFQ61_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954220.1"
                     /translation="MNSIIAKSYWAIAAALALGCSHNEDRNTTPSDTSGAFTNSTGAS
                     EGTAPGPAAPGAGDPGAPAGTGTGTGSGTSPPDNSSSPTGPTSQLQAGGNGGVGGGGG
                     GVGGMGGLGGAGNTAPAANAAHPTGAPGSSGNMAPGNRSSAGRPGGIH"
     gene            complement(4099..5214)
                     /locus_tag="VFQ61_06945"
     CDS             complement(4099..5214)
                     /locus_tag="VFQ61_06945"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9954221.1"
                     /translation="MKYRVLITGGAGFIGSRLADALLSRGYAVRVLDVLSPHVHGRAQ
                     QRPPRLSSDVDLCVGDVRDARAVARALEGVDAVFHLAALCGVNPSMYKVHDYVDVNAR
                     GTAVLLEGLARRPVRKLIVGSSMSVYGEGQYQDADGRVVCAGRRSASQLKRSEWELSD
                     ASGAPLQAVATPEYKVPAPESIYALSKFHQEQQCLIAGRAYDIPTTVLRFSTVYGPGQ
                     TVNGAHSGVMSMFASRFATGRAPLIYEDGLQKRDFITVDDAAEACALTLTEPKSDGLT
                     INIGSGEGRTILDVASRLAVALGKTPAAEVSGRYRLGEVRHCFADIALARDVLGFSPR
                     VSFEEGIEEFAASVQSSWKDEDGALEDETEVRFGGLS"
     gene            5542..6609
                     /gene="coxB"
                     /locus_tag="VFQ61_06950"
     CDS             5542..6609
                     /gene="coxB"
                     /locus_tag="VFQ61_06950"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET9954222.1"
                     /translation="MPGRVLLQLVAGGSLVACGSLSAPEQSSALRPISAESRALFELG
                     SLFFWTTLAVWVAVVFAALWGTYRGLKRANARPAIEPPPEETAQRNSKAIEASGQRAA
                     EWAVAASTVVSICILFGLLIASIRTGRALAAQPDETALHIRITAHQWWWEINYSGGDL
                     QQSFTTASELHLPAGRRVELELTSADVIHSFWVPNLHGKRDLIPSHITRLSFRPDQTG
                     TFRGHCAEFCGLQHAHMDLPVVVETPTRFASWYEHERSNASVPHDPETQRGREIFMAG
                     PCAFCHNVRGTDARGSVGPDLSHFGSRPELGAGAAKNTPESLKRWITQTQHLKPGTKM
                     PATVLSQVELDALARYLQSLK"
     gene            6606..8582
                     /gene="ctaD"
                     /locus_tag="VFQ61_06955"
     CDS             6606..8582
                     /gene="ctaD"
                     /locus_tag="VFQ61_06955"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET9954223.1"
                     /translation="MTESAIDPTATHASVPRRHSQDLELAADEPEREADRTPLTRELR
                     RTWQHASGFWGWFQEVSSLVIGLRTIVTAFAFFALGGLEALLMRLQLARPDAGLIDAD
                     TYSQLFSTHGTTMMFLFGVPLGQGLAIYLVPLMLGTRSLIFPRLNAYAYHTYLIGGLL
                     LYTGLALRSAPDAGWFSYVPLAGKLFSHGKRVDIWAQTVTFTELASLAIAANVIVTVL
                     KTRAPGMSLSRIPLFVWAQLVTSFMILFAMPAVMVASTMLATDRLVNTQFFNAAAGGD
                     PLLWQHLFWFFGHPEVYIIFLPALGVVSAIVETFAGRAIIGYVPMVLSLVVTGFMGFG
                     LWVHHMFATGLPQIGQSFFTASSMIIAIPTGIQIFCWIATLWRGKLVLKTPLLFVLGF
                     IGTFVIGGLTGVMLASVPLNVQVHDTFFVVAHLHYVLIGGAVFPVLGGIYYWFPKFSG
                     RFLDERLGLVNFILIFLGFQLTFFPMHVLGLQGMPRRIYTYSAETGWQPLNFLATIGA
                     VVLGLGVLCLVLNVILSLRSGARAGANPWRAASLEWSASSPPEPFSFGALPSVAGRQP
                     LWEAERPEVSGLETKERAVLVTDALDARPDHRYLLPGPSNTPFLAAVATAVMFIGLIF
                     TPWAVPVGALLLGAVLTSWFWPRRPADELLEQQP"
     gene            8579..9157
                     /locus_tag="VFQ61_06960"
     CDS             8579..9157
                     /locus_tag="VFQ61_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954224.1"
                     /translation="MTQAAQTHGPSLASPSMQPKTPGIGTIDVSGLPTYAFGKRDPLF
                     WGALTLVAIEGMSILLVYFSLLYVRQRVVGWPLAAPTPLGTWLSAGIVLTLSVSALPN
                     WALSRAAQRGALRTMQVQMVLLSALGLLACVLRGFEFAALPFRWDANIYGSLFWAALV
                     LNTVHLGGGALKTWSFSDCSSRDRFNRSISPI"
     gene            9267..9776
                     /locus_tag="VFQ61_06965"
     CDS             9267..9776
                     /locus_tag="VFQ61_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954225.1"
                     /translation="MTVHSPFTRDPRDSSDHHAKDYREPSTRLAQSSPLEPRDRWLLT
                     TALIPPAAWAVNLSLAYPLVQELARPEHKVFVYGLSVLSLVLTLGSGLVAYGIWSSTR
                     REAAAAPEILAQSTPSDRSALDAPSSGSAPLRSSQTILAFAGMGLSVLFTLAIVAQII
                     PLLFFALQE"
     gene            9811..10854
                     /locus_tag="VFQ61_06970"
     CDS             9811..10854
                     /locus_tag="VFQ61_06970"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET9954226.1"
                     /translation="MTCISASALDMSGLHMSRLYLNEAALWLAHPNTGLSSVPWEIDP
                     YGVAAVLPTGLIYGVGLSRVWAKTGVGQGIAVWRALAFGIGWTVLTFALLSPLDSLSD
                     LLFSAHMLQHELLMLVVAPLMILGRPMLAALWILPRATRLKVTQASNRTWAQVCWGFL
                     STPWFALALHGLVRWIWHAPALFEGAMRNPSWHAFQHFTFFASAALFFRVLFEGRYGK
                     AGYGVAMLFVFATALHTSVLGALFALGPHVAYAIYALRSPLVGQNALEDQELAGLLMW
                     IPSGFVFAGIGLALFGAWLGEIEKRAELVNKRRQAGAVTVSATEASMESDSRFHSPGA
                     EETARPDRGTHLA"
     gene            10885..>11897
                     /locus_tag="VFQ61_06975"
     CDS             10885..>11897
                     /locus_tag="VFQ61_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HET9954227.1"
                     /translation="MSETQRPHVLLRDSQAAASAASLRYVCAEELEILRERKGRGFVY
                     RGPNGRVIKNAKVLARIRQLALPPAWKQVRISMRDDGHLQAIGIDAKGRKQYRYHPAW
                     RQVRDLAKYDNIVRFVRALPRLRERLSQDLKQRALSKTKVVAAVVSLMERTQLRVGNR
                     RYAEQNGSYGLTTLRDEHARISGASLTLRFRGKSGKQFDVNLHDRRLAKIVRKCRDIP
                     GQTLFQYFDEDGQHHAVTSCDVNAYLQETMGHAFTAKEFRTWAASSLAALHLASSLPF
                     GSQTAAKRMLVAAAKMVASELGNTPAICRKCYIHPGIFDCYLRGQLHESLDALLSRAR
                     KRRP"
BASE COUNT         2113 a         3707 c         3634 g         2443 t
ORIGIN      
        1 cgcgcgcgct cgccggtagc gggacggcga tatggcctcg tcgagcgcgc catctgaaca
       61 ccattcagat ggcgccaatc taagtggcgt caagatgggc gggacgccgc ccccgacgcc
      121 ggggtatggc cggccatggt ataagctgca gccatggctc ggccgcgaac ccgatcgctc
      181 ccgcagaagg agagctacca tcacggagat ctccgccatg ccctcctgga aagcgcactc
      241 gcgatcgtct ccgaggaggg tgtccacgac ctctccttgc gagaagtggc ccgtcgcgcg
      301 ggggtgacct acgccgcgcc gtaccatcac ttcgccgaca aatccgcgct cttagccgcg
      361 gtgggcgcgc agggcttcga ggtgctgatt cgtgagctcg agcgggcgag ctcgcggagc
      421 gaggacttgc gggggcagct cctcgccatg gcggaggcct acttggcgtt cgcgcgcagc
      481 cacccctcgc actatcgcgt gatgtttctg cccgaactca agatcacgga cgtcgaggag
      541 tatcatcgcg ccggggaccg tgccttcgac atgctggtgt cccgcgtgaa gctcgcgcgt
      601 ccgggcgatc cagagacggc acagctcacg gtcgcggcca cggtctgggc agcgctgcac
      661 gggttgtcac ttctcagcat cgacgggacg ctcgagcata aattcgccaa gatcaataag
      721 atgttgcgcc aagcctgcgg cgccatcgtg cgcatggtgc tttgagcgtc ttggctggcg
      781 cgaatcgccg gcatcatctt agccgggggc taaccttaat tcggacgcgc actcgtcggg
      841 aggctcgata ttgcgggccg gaatgtagca aactgccggc catgttcgtg attgagctca
      901 tatacaaagt acctctggcc gagatcgacg cctgcatgaa cgatcacgtg gccttcctga
      961 agagacacta cgattcgggg aacttcctga tctcggggag aaaaatcccc cgcgatggcg
     1021 gagtcatcct ggcgttgggg gccagccgcg aagccgtgga agccatcatt cgtgaggatc
     1081 cgttcgtctc gcgtggcctc tccgactatc gcatcatcga attcagggcg agcttcaccg
     1141 cggatgacat gcccaaacgg gtccgcggct gaccctgaac ttccggcaca ggagcggaca
     1201 cgcgtccgct tccgtgccgc gacaattgtc ctttcacgga ctaatgtcct tggtcattcg
     1261 attgagaatc gcgctgacgc agcgtgtttc tcgtgcattt cagataccca ttggcccgta
     1321 actaagccaa cgggaacctg gcccgtcgcg tgcagcgcaa tcgccctttc acgacttgcg
     1381 gaggcgcata gtggattgtt cgagtttgag cccggcgcag ctcatcgaga tactgtgcaa
     1441 tcgacgccga accgccgagt tggtggccac cctcgagccg gtggtgcgaa cgcgcgtcac
     1501 gcgcacgttg tgccgctttg gtcgctgcgg gcacgaagag atcgaggatg ccactcagga
     1561 agtgctgatc gagctcttta ccaaagacgc gcgtacactg cggagctggc agcccgagcg
     1621 aggtctgtcg ctcttgggct tcgtgcagtt cgtcgcgcac cgagtggcgt tggctcggct
     1681 caagcaagtt tggctcgatc gcgcccagca accgctcgag gcgtgcgaag atcgggcagc
     1741 ggactcggtg ctgcggcccg atcgaaagct agctacgcgc cgccagttcg agcgcctact
     1801 ggagcgactg caggtcgaag tctcgccgcg tggcgtgcag ttgttccgcg ctctattcat
     1861 ggagcagcat tctctgtcgc gcgtatgcag tgaatttggg ctacagccag atgccgttta
     1921 cgcttggcgc agccggctga gtcggcgggc gcgcaatatt tgtgcagagc tcgagccgcc
     1981 gtccacccca cgcacggcat agtcctgaat cgttcatcgc gggcgcgggc gcggttcttg
     2041 gggggcatgg ggttcgcggg gctgcgcttt cgaggcgctc gactggcttc gcgctcgtcc
     2101 accacgtggc gaaaaacaga agcgggcaag atcgctgtcg atcttgcccg ctcgaagttg
     2161 cctcggaatg gttagtgaag gaagctcaga cgggccgccg ccggatatcg ctacttgcag
     2221 cttccggcat gatcgatctg ggcaccgacc atttgtgcga agcaagagtt gctataagtc
     2281 ttgccgtcgc agccgcacac cgggtcgaag atctccaagc aaacctgcgg gaacggtcgg
     2341 cactcgccgc ccgcgtcggc cgtcaggcat tgaccaacgc cgaagtcgca gtactcacct
     2401 tcatcgcagc tcagccccac gatggtgccg cagactttgg gctccggtgc gcaaggtccg
     2461 tcgaattcta cgctgacgcc gctcgcggcc gcttggcaag cattggagta ggtcacccca
     2521 tcgcagccac ataccggctt gaactccttg gtgcagattt ggggcttgga agcgcaaact
     2581 ccgggagcat cggcgcgccc gcaattggct cccggcggga agtcgcagta ctcgcccgcg
     2641 gcgcattcgg agaggccgcg cgagccgcat gcagccgggc ctttgccctt gaagggtaga
     2701 tagtattcac taccaatcag cgcaaagcct tggcccgcgg ggccggtgac ggtcttacgt
     2761 gaagccgcga gcaggagccc ttccggctcg tgaagttggg cgaatccgtc gctgggatcc
     2821 gagctcacgc cgctcagatc gatctgggcc acgctcgcgg gggcggtact cgaattgagc
     2881 agctgggcct tgaacgaggg gcacgggaag gtgatgcata cgatgcccgt ctcgttggct
     2941 tggaagaacg agccgctcgg tgtgacgccg gggtggccgt gataagcatc gagtacacgg
     3001 agcgtcccga aggtgcccag ggaaacgaag tccttcggtc caattgcgcc ccgaagcagg
     3061 aactcggtcg acgagccacg caccacttgc tcgctgtccg cgtcgagagc gagcgcggag
     3121 agatccaggt cggcaacgta gcactcggct tgtgaggtcc cgtcgacaca gcgcgtgagc
     3181 ttttgattca cggctttgac gaagtagccg ccgcaacggg ggaacgcgca gcgcctgagg
     3241 tcgggcctca acgtgaagta actcgcgacc ggcgccttgg cgagcgcctg ctgcgctgag
     3301 tctgagtctc cgctagctgc gccgagatct tctgcaccgc tcatttccgg tgcggtgctc
     3361 gaacatgcgg ccactagcca acaggctgcg gcactgaaca acagattcca attcttgctc
     3421 atggtctact ccactcaaca gtctatccaa ccgttcgtct agatagtgca gctcgcgacg
     3481 cggcaagcct cgtcgtaccc gtacgagcga catctgctga atggattgtt cggagcagcc
     3541 tagtttctga caacgtccca ggctgccccg tgaagatcga gcaagctaag tcctattagg
     3601 atcgcagtgc tcagcagcgc tctgggccgg cgagcactgc tctcccaact cagtggatcc
     3661 ccccgggccg gcctgccgag ctgcggtttc cgggcgccat gttgcccgaa ctgcctggag
     3721 cgcccgtggg atgcgcggcg ttggcggcgg gagcggtgtt gccagccccg ccgagtccac
     3781 ccattcctcc cacgcctcct ccgccaccgc ccactccgcc gttccctccg gcttggagtt
     3841 gagatgtggg gccagttggg gagctgctgt tatccggtgg tgatgttcca ctgcccgtcc
     3901 cggtgccggt cccggcggga gcgccaggat ctccagcgcc cggcgcggca gggcccggcg
     3961 ccgtgccttc gctcgctccc gtggagttag tgaaagcacc tgaagtgtca ctgggagtcg
     4021 tattgcgatc ttcgttgtga ctgcagccga gcgccagcgc ggcggcgatg gcccagtaac
     4081 ttttagcaat tatcgaattc atgacagccc tccgaatctg acttccgtct cgtcctcgag
     4141 cgcaccgtcc tcatccttcc aggagctctg aacagaggcg gcgaactcct cgatgccttc
     4201 ttcgaacgag actcgcgggc tgaagccgag cacgtcgcga gccaaggcaa tgtctgcaaa
     4261 gcagtggcgt acttccccga gtcgatatcg accgctcacc tcagcggccg gggttttccc
     4321 gagggctacg gcgagacgtg aggcgacgtc caaaatcgtg cggccctcgc cactaccgat
     4381 attgatcgtg aggccgtcac tcttgggctc cgtcagtgtg agagcacagg cttcagccgc
     4441 gtcgtccacg gtgatgaagt cccttttctg caggccatct tcgtaaatca acggcgcgcg
     4501 cccggtcgca aaccgcgatg cgaacatcga catcacgccg gagtgcgcgc cattcacagt
     4561 ctggcccggc ccatagacgg tcgagaacct caggaccgtg gtcgggatgt cgtaagcgcg
     4621 ccccgcaatc aggcactgct gctcttgatg aaacttggat agcgcataga tcgattcggg
     4681 cgcaggaacc ttgtattccg gtgtagcgac ggcttgcagc ggggccccac tggcgtcgct
     4741 caactcccat tcgctgcgct tgagctgaga cgcgctgcgg cggcccgcac acaccacacg
     4801 tccgtcggcg tcctgatatt gaccttcgcc gtacacactc atgctcgagc ctacgatcag
     4861 cttgcgaacg gggcgccgcg ccaggccttc gagcaacacc gcggtgccgc gtgcgttcac
     4921 gtccacgtag tcgtgcacct tgtacatgct ggggttcacg ccgcacagcg ccgccaggtg
     4981 aaatactgca tccacacctt ctagggcgcg cgcgacggct cgcgcatctc gcacgtcccc
     5041 aacacacaag tccacgtccg aggacaatcg cggaggccgc tgctgggcgc ggccgtgaac
     5101 gtgcgggctc agcacgtcga gcacgcgaac ggcgtaacct cgcgacagca gcgcgtcagc
     5161 caagcgcgaa ccgatgaaac ctgctccacc agtgatcaag acgcgatact tcatgaacgg
     5221 gaactcctcc tggacaagcc acgctgatgc aatgcgcgat ccataacggc tcgttgaacc
     5281 tgggtcattt gactgcgtag attcagccat tctccgtcaa ctcgaggagt gacagaggaa
     5341 cggctttcga tccacggcgt cgaatgtgga gcgatgtgtc agcaattacg atccgaaagc
     5401 ggcaaaagcg ccgcaaggct tagacatcac tcacgtcgga tgcagatcgc cacacagggc
     5461 atgtgctttg cacgatgttt cgactagatg gtcacttcgc accgcgtgtt ttgccgctgt
     5521 gcacgtgtat cagtacaccg agtgcccggg cgagtgctat tgcaactcgt tgcgggcggc
     5581 tcgctggtcg cgtgcggctc gctctcggca ccggagcaga gcagcgcttt gcgcccaata
     5641 tctgctgaat ctcgcgcgct tttcgagctc ggcagcctat tcttctggac cacgctcgcg
     5701 gtctgggttg cggtcgtgtt cgcggccctg tggggaacgt atcgaggatt gaagagagcc
     5761 aacgctcgac ccgccatcga gccaccgccc gaggaaaccg cgcagaggaa ctcgaaagcc
     5821 atcgaagctt ccggacagag agcggcagaa tgggccgttg ccgcttcgac ggtagtctcg
     5881 atctgtatcc tgttcggcct gctgatcgcg agcattcgca cgggacgggc gttagcggcc
     5941 cagcccgatg aaaccgcctt gcatatacga attacggcac atcaatggtg gtgggaaatc
     6001 aactactccg gcggtgacct gcaacagtct ttcacgactg cctccgagct gcatctgccg
     6061 gccgggcgcc gcgtcgaact cgaactcacc tccgcagacg tgattcacag tttttgggtg
     6121 ccaaacctgc acggcaaacg cgacttgatt ccgagtcata tcacgcgctt gtccttccgt
     6181 ccggatcaga ctggcacgtt tcgcggacat tgtgcggagt tctgcggact gcaacacgct
     6241 catatggatt tgccagtagt cgtagaaacc ccaacacgct ttgcgagctg gtacgaacac
     6301 gagcgctcca acgcaagtgt cccccatgac ccagagaccc aacgcggccg cgagatcttc
     6361 atggctggcc cgtgcgcctt ctgtcacaac gtgcgcggga ccgacgcgcg aggctcagtg
     6421 ggccccgact tgagccactt cggctcgcga ccggagcttg gcgcgggggc agcgaagaat
     6481 acgcccgagt ccttgaagcg ctggatcacc caaacacaac atctcaagcc cggcaccaaa
     6541 atgccggcca ccgtgctctc ccaagtggag ctcgatgcct tagctcgata tctgcagagc
     6601 ttgaaatgac cgagtcagcc atcgacccca ccgctaccca cgccagtgtc ccgcggcgcc
     6661 attcccaaga tttggagttg gccgcagatg aacccgagcg ggaggcggat cgaacgccgc
     6721 tcactcgtga gctgagaaga acctggcagc atgcgagtgg cttttggggc tggtttcagg
     6781 aggtgagcag cctggttatc gggcttcgca cgatcgtcac ggccttcgcg ttctttgcgc
     6841 tcggcggact ggaagcactg ttgatgcgcc tccagctcgc acgaccagat gccggtttga
     6901 tcgacgccga tacgtatagt cagctcttct ccacacacgg caccaccatg atgtttctgt
     6961 tcggagtgcc gctcggacag ggactggcca tctatctcgt gcctttgatg ctcggcacac
     7021 gcagtctgat tttccctcgc ctgaacgctt acgcttacca tacgtacttg attggggggc
     7081 tgctgctcta tacgggactg gccctccgat ccgcaccgga cgcgggttgg ttcagctacg
     7141 tgcccttggc gggcaagctc ttctcccacg gcaagcgcgt cgacatttgg gctcagacgg
     7201 tcactttcac agagctcgcg tcgctcgcga ttgcggcaaa cgtcatcgtt acggtgttga
     7261 agacccgcgc cccgggcatg tcgctgagcc gcatcccgct gttcgtttgg gcgcagctcg
     7321 tcacctcgtt catgattctg ttcgcgatgc cggctgtgat ggtggcaagc acgatgttgg
     7381 caacggatcg gctcgtcaac acgcagtttt tcaacgcggc cgcgggcggg gatccgctgc
     7441 tttggcaaca tctattctgg ttcttcggtc atcccgaggt ttacatcatc ttcttgccgg
     7501 cgttgggggt ggtgtccgcg atcgtggaga ccttcgcagg ccgtgcgatc attggttacg
     7561 ttccaatggt gctgtcgctc gtggtgaccg gcttcatggg cttcggcttg tgggtccacc
     7621 acatgttcgc aaccgggctg ccgcagattg gtcagagctt ctttaccgct tcgagcatga
     7681 tcatcgccat cccaactggg attcagatct tctgttggat cgcgaccctt tggcggggaa
     7741 agctcgttct caagacgcca ttactcttcg tactgggctt catcggcacc ttcgtgatcg
     7801 gtggcttgac gggcgtgatg ctcgcctcgg tgccgttgaa cgttcaagtt cacgacacct
     7861 tcttcgtggt tgcgcatctt cattacgtac tgattggcgg cgcggtattt ccggtgctcg
     7921 gcgggattta ctactggttt cccaagttca gcgggcgatt tctcgatgag cggctcgggc
     7981 tagtcaactt catcctgatc ttcctgggtt ttcagctcac gttctttccc atgcacgtgc
     8041 tcggcttgca gggaatgccg cgacgcattt acacctactc ggccgagacc ggctggcagc
     8101 cgctgaattt cctagcgacg attggggccg tagttctggg cttgggtgtg ctgtgcctgg
     8161 tcctgaacgt gattctgagt ttgcggagcg gcgcccgcgc gggcgcgaac ccatggcggg
     8221 ccgcatccct cgagtggagc gccagctctc ctcccgaacc tttcagtttc ggagcgctac
     8281 cttcagtggc cggccgtcaa ccgctgtggg aggcggagcg accggaagtc agcgggctcg
     8341 agaccaagga acgtgcggta ctcgtcacgg acgcactcga tgctcgcccg gatcaccgct
     8401 atttgttacc ggggcccagc aatacgccct tcctggccgc ggtcgcgacg gcagtgatgt
     8461 tcatcggtct gattttcacg ccgtgggccg tgccggttgg cgccttgctc ttgggagccg
     8521 tactcacctc ctggttctgg ccacgtcggc ccgccgacga gctgctggag caacaaccat
     8581 gacccaggcc gctcagacgc acgggcccag tttggctagc ccgagcatgc agcccaagac
     8641 tcccggcatc ggaacaatcg acgtgagcgg tttgcccact tatgccttcg gcaagcgcga
     8701 tccactattc tggggtgcgc tcactttggt ggcgatcgag ggcatgagca tcttgctcgt
     8761 gtatttttcg ctgctgtacg ttcgtcaacg cgtggtcggg tggccactcg ccgcgcccac
     8821 tccgctcgga acttggctgt cggcggggat cgtactcacc ctttcggtca gcgcactgcc
     8881 gaattgggcg ttgtcgagag cggcccagcg cggagcctta cgcaccatgc aggtgcagat
     8941 ggtcttgctc agcgcattgg gtctgctcgc gtgtgtgctt cgtggcttcg agttcgcggc
     9001 tttgcccttc cgctgggatg ccaacatcta tggctccctg ttctgggccg cgttggtctt
     9061 gaatacggtt catctcggcg gcggagcgtt gaaaacttgg tctttctcgg attgctcttc
     9121 aagggaccga ttcaacagaa gcatctcgcc gatctaacgg cgaatagcat ctattggtac
     9181 ttcgtggtct tgagttggat tcctcagttt tgcttgctct atctcgaacc ggcactcctc
     9241 ctcgctcgtc agccatgagg ctcgcaatga ctgtccattc tccgtttacc cgcgatccac
     9301 gggattcatc ggaccatcac gcgaaggact atcgcgagcc gagcacgcgg ctcgcgcaga
     9361 gctcgccgct cgagccgcgc gatcgctggt tgctcacgac ggcactgata ccgccggcgg
     9421 cttgggccgt gaacttgagc ctggcctacc ctctcgtcca agagctcgcg cgccccgagc
     9481 acaaggtctt cgtctacggt ctgtcggtat tgagcctggt attgacgctc ggttcgggct
     9541 tagtggcata cgggatttgg tcttcgacgc ggcgcgaggc agccgctgca cccgagatcc
     9601 tcgcgcaatc aacgccgtcg gatcgatcag ctttggacgc ccccagcagc ggctctgccc
     9661 ctctccgtag cagccaaaca atcttggcct ttgccggaat gggattgagt gtactgttca
     9721 cgttggcgat cgtcgctcag atcattccac ttctattctt cgcgttgcag gaatgagcgc
     9781 agcgaggaat gggaggggtt gggagtctac atgacttgca tctctgcaag cgcactggac
     9841 atgagcggcc tgcacatgag ccgtctttac ctgaacgaag ctgccctctg gcttgcccat
     9901 cccaacaccg ggctttcctc ggtgccttgg gaaatcgatc cgtatggagt cgccgcggtc
     9961 cttccgaccg gcttgatcta cggcgtcggg ctgagtcgcg tctgggccaa aaccggggtc
    10021 ggccagggga tcgcggtgtg gcgcgcgcta gcgttcggga tcggttggac cgtgctcacg
    10081 ttcgcactgc tctcgcccct cgactcgctc agcgacttgc tattctcggc gcacatgctc
    10141 cagcacgaac tcctgatgct ggtcgtggct ccgctgatga tcttgggccg gccgatgctc
    10201 gcggcattgt ggatcttgcc gcgtgcgacg cgtttgaagg tgacccaagc ctccaatcga
    10261 acctgggccc aggtttgttg gggatttctg agtacgccct ggttcgccct cgctttgcac
    10321 ggattggtgc gttggatttg gcacgccccg gcgctgttcg agggtgccat gcgaaatccg
    10381 tcctggcacg cctttcagca ctttacgttc ttcgcgagcg cagcgctctt ctttcgagtg
    10441 ctgttcgaag ggcgttacgg gaaagccgga tacggggtcg ctatgctgtt cgtgttcgcc
    10501 acagcgctgc acacgagtgt gctcggcgcg ttgttcgcac tgggcccaca cgtggcatat
    10561 gcgatttacg ctttgcgttc gccgctcgtg gggcagaatg ccctggagga ccaggaactc
    10621 gcgggactct tgatgtggat cccctcagga ttcgtgttcg ccggaatcgg tctcgccctg
    10681 tttggtgcct ggctgggaga aatcgaaaag cgcgccgagc tcgtcaataa gcggaggcag
    10741 gcaggcgcgg tgaccgtcag cgccacagag gcatctatgg agtccgactc gcgattccac
    10801 tcgcccgggg ccgaggagac ggcccgaccc gaccgcggca cacaccttgc ctaaaccgct
    10861 gtgaagctct tagaggccag cgccatgtca gaaactcagc gtccgcacgt tctgcttcga
    10921 gattcgcagg cggccgccag cgcggcgagc ctgcgctatg tgtgtgccga agaactggaa
    10981 attctgcgcg aaagaaaggg tcgtggcttc gtttaccgcg gcccgaacgg tcgggtgatc
    11041 aagaatgcga aggtcctcgc ccgtattcgc cagctcgcgt tgccacccgc ctggaagcaa
    11101 gtgcggatct cgatgcgcga cgacggacac cttcaagcaa tcgggatcga tgctaaaggc
    11161 cgtaagcaat accggtatca tcccgcttgg cgacaagtgc gtgatttggc caaatacgac
    11221 aatatcgtgc gtttcgtgcg agcactgcct cgcttgcgcg agcggctctc gcaagatctc
    11281 aagcagcgcg ctctcagcaa gacgaaggtc gtggcggcgg tggtatcgct gatggaacgc
    11341 acccagttgc gcgttggaaa ccggcgctac gccgagcaaa atgggtccta cggcctgacc
    11401 acattgcgcg atgaacacgc tcgcatctcg ggcgcgagtc tgacgctgcg ttttcgcggc
    11461 aagagcggga agcaattcga cgtgaacctg cacgatcggc ggctcgccaa aatcgtacgc
    11521 aagtgccgag acattcccgg acaaacccta tttcagtatt tcgacgagga tgggcaacac
    11581 cacgccgtaa cctcttgcga cgtaaatgcc tatctccaag agacgatggg gcacgctttc
    11641 actgctaaag agttcagaac ctgggccgcg agctcactag ccgctctgca cctcgccagc
    11701 tccctgccct tcggcagtca aacggcagct aaacgcatgt tggtagcggc cgccaagatg
    11761 gtggcttcgg aactcggcaa tacacccgcg atctgccgaa agtgctacat ccatcctggc
    11821 atcttcgact gctatttgcg cgggcaactc cacgagtcgt tggatgcgct gctctctcgc
    11881 gcacgtaagc ggcgccc
//
LOCUS       DASTKU010000183         3821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_703607, whole genome shotgun sequence.
ACCESSION   DASTKU010000183 DASTKU010000000
VERSION     DASTKU010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703607"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFQ61_06980"
     CDS             <1..313
                     /locus_tag="VFQ61_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET9954228.1"
                     /translation="GTELQARADALISFFHSKGPKVYGSLWETDGSQGGGFHSCGLVA
                     MNATATLATSAAVAPKAAEIVEDFWNYPLPVGQYRYYDGLLYMFGFLHLSGQYRIYAG
                     Q"
     gene            complement(417..1028)
                     /locus_tag="VFQ61_06985"
     CDS             complement(417..1028)
                     /locus_tag="VFQ61_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HET9954229.1"
                     /translation="MIHVYGHPLSTCTRKVLTSLVETNTPYELHVVDFAKNEHKQEPH
                     ILRQPFGQVPAINDEGFELFESRAICRYISRKADNRLTPADPKACALMDQWLSIEQSN
                     FSPAAMKFVYHYVFQRPQEDSVLEGATKMLETCYGVMSSALEKSSFLVGEQFTIADIG
                     YMPYIEYMLGSPAKATLDKFPRVSAWWQRVSERPSWRKVTGKG"
     gene            1331..1513
                     /gene="yacG"
                     /locus_tag="VFQ61_06990"
     CDS             1331..1513
                     /gene="yacG"
                     /locus_tag="VFQ61_06990"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HET9954230.1"
                     /translation="MRIECPTCKTILEDVPDDYASRPFCSARCKLVDLHAWLNEEYRV
                     SEPLPIDVEDDRRRLN"
     gene            complement(1627..2592)
                     /locus_tag="VFQ61_06995"
     CDS             complement(1627..2592)
                     /locus_tag="VFQ61_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9954231.1"
                     /translation="MRQFRSVLAYVDTRNNSMAAVQTAVDVAKRGASRVLAMRVVDLD
                     LAPLSPHLGGNDWENYLLRVALVEQRALQAKVEAYRATVPVSAQVRVGKPSLELIREV
                     MAAGHDLVIKVASGTANQQRVAFGSTGMHLVRKCPAPVWLTPADATPPRRILCAVNPG
                     PEGDEERRRLSCKIVAHGLALARHFDARLDIVHALRPSSYAAVGHPQVVEGRASISRR
                     LESLADTVIRESGLGFAGVRVQVLDGEPEIAISELAMDGLATLIVLGAIGRPGIAGLL
                     IGELSEDILLRVRCGVFCVKPDGFVSPLLGLATPTYEPRQLSEEA"
     gene            complement(2823..3638)
                     /locus_tag="VFQ61_07000"
     CDS             complement(2823..3638)
                     /locus_tag="VFQ61_07000"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9954232.1"
                     /translation="MRLDFGRLGMIRGGLLIVTLTAAACSAETGANGPERTGTPEVYG
                     GDVDTAHPAVAYLVGSTDNENGTIGTSTCTGTLIAPTVILTAGHCLVVYNRDVKRARF
                     EMDREHAKWIDIVDFRMHPEYDSHASSEGHDIAVALLAEPAPVAPLPYNREALRAELV
                     GQPLRVVGYGDSDDFEPEAGIKRQALISLTSFSDKWLESGDLDHHTLEGDSGGPALMN
                     LRGVETIVGVLSRGEYPRDQPFGSSRVARQFHTRVDADADFIDAFVESVRSEH"
BASE COUNT          709 a         1232 c         1164 g          716 t
ORIGIN      
        1 cggcacggag ctgcaagcgc gcgcagacgc actgatcagc ttcttccata gcaagggacc
       61 caaggtgtat ggctcgctgt gggagacgga cgggtcacaa ggcggcggct tccactcttg
      121 cggcttggtg gcgatgaatg ccaccgcgac cctcgcgacc agcgccgcgg tggcgcccaa
      181 ggcggcagaa atcgtcgagg acttctggaa ttacccgctg cccgtcggac aataccgtta
      241 ctacgacggc ctgctctaca tgttcgggtt cctgcacctg agcgggcaat atcgaatcta
      301 cgccggtcag taatcgatat ccgctggacc tttcgatatc gacgggtgac tgcagcgcgt
      361 cttgaacgag acgcgctgca ggactacggc cgagctcgac gccgggcgcg gtttgctcag
      421 cccttgcccg tgactttgcg ccagctcggg cgctcactga cgcgctgcca ccaagcggac
      481 acgcgtggga acttgtcgag cgttgccttg gcggggctgc ccagcatgta ctcgatgtac
      541 ggcatatagc cgatatcggc gatcgtgaac tgctcaccga ccaagaacga gctcttctcc
      601 agggcgctcg acatcacgcc gtagcaggtt tcgagcatct tggtcgcgcc ctccagcacc
      661 gaatcctcct ggggtcgctg aaagacgtag tgataaacga acttcatcgc cgccggcgag
      721 aagttggatt gctcgatgct caaccactga tccatcagcg cacaggcttt tggatcggcg
      781 ggcgtgagac ggttatccgc tttgcggctg atgtagcgac agatcgcccg cgactcgaag
      841 agctcgaagc cctcgtcatt gatggcaggc acttgaccga agggctgccg gagtatgtgc
      901 ggctcttgtt tgtgttcgtt cttggcgaag tccactacgt gcagctcgta gggagtgttg
      961 gtctccacca gcgatgtcag aaccttgcgc gtacaggtgc tcagcgggtg tccataaacg
     1021 tggatcatcg ggtacctccg aggcgggcag gttagcgtga attcgtttcg ttctactacg
     1081 cccttcgcgc ggcgagccgc gaaggcgtgc tgtgcgttgg gcccgcgacg cgaccgtcaa
     1141 cctgcccttt cggttgggct gcctcgtgac cgccgccgaa agtcgccgca gacgcgctga
     1201 gcgacggcaa ttttctcctt cccggggtca aagggatagg tgtgacgtga aggcccatcg
     1261 aagtcgggcc gttgaagctc tagctctgcg cgtcccagac atcccgccgc gaggtgctaa
     1321 ggttgcccgc gtgcgcatcg agtgccctac gtgcaagacg attttggaag acgtgccgga
     1381 tgactacgca tcgcgccctt tttgttccgc gcgctgcaag ctggtcgatc tgcatgcgtg
     1441 gctcaatgag gagtatcgcg tgtcggagcc gttaccgatc gacgtcgaag acgatcgtcg
     1501 ccggctgaac tgacgccgag ggggcttccc gaaaccaggc tcgcggtttc ggatcgggca
     1561 gcctcggcgc gcggctcctc gagctcgagc cagagtccaa gcggaccgac tctgcggctc
     1621 gtgacttcac gcctcctcgg agagctggcg tggctcgtaa gtcggagtcg cgagcccaag
     1681 caagggagag acgaaaccat ccggcttgac gcaaaagact ccacatcgga cccgcaacag
     1741 gatgtcctcc gagagctcgc cgatcagcaa ccccgcgata cccggtcgcc cgatcgcccc
     1801 cagaacgatc agggtcgcga gcccatccat cgcgagctcc gaaatcgcga tctcgggctc
     1861 gccgtcgagc acctgcacgc gaacgccagc gaagcccagg ccggactcac ggatcaccgt
     1921 atctgccagc gactccagcc gccgtgagat gctcgctctc ccctccacta cctgggggtg
     1981 tcccacggct gcgtaggacg atggccgaag cgcgtgcacg atatcgagcc gcgcatcgaa
     2041 gtgtcgcgcc aacgccaatc cgtgcgccac gatcttgcac gacaagcgac gccgctcctc
     2101 gtctccctcg gggcctggat tcaccgcgca caggattcgt cgcgggggtg tggcgtcagc
     2161 cggagtcaac caaaccggcg ccgggcactt gcgcacgagg tgcatccccg tacttccaaa
     2221 ggcgacgcgc tgctggttcg cggtgccgga cgcgaccttg atcaccaagt cgtgcccagc
     2281 cgccatcacc tcgcggatga gctccaaaga gggcttgccg acgcgcactt gagcggagac
     2341 gggcacggtc gcgcggtaag cctccacttt ggcttggagc gcgcgctgtt ccacgagcgc
     2401 gactcgtagc aggtaattct cccaatcgtt gcccccgagg tgcggtgaaa gaggagccaa
     2461 gtcgagatcc accacccgca tcgcgagaac cctgctcgcc cccctcttgg ctacgtccac
     2521 ggccgtctgc acggcggcca tactgttgtt gcgagtgtct acgtacgcga gcactgaacg
     2581 gaactggcgc atgagaactc ccttctggat tgaggtgctc aagagatgag gcgcgccgtg
     2641 ccgtttgaaa attatgaaat tcgagccgca tccgtagcca aacgttacat gacgtgcgca
     2701 ggcgaggctt ctccgtgact cgggcgcggc cggctcacct agctgcgcgc gcctccgatg
     2761 cgtggatgat caccggaacc tcggcgcaac acgagcaggc gctctgaagt ctgcgggcgc
     2821 tcctagtgct cgcttcgaac cgactctacg aaggcatcga tgaagtcggc gtcggcgtcg
     2881 acccgggtgt gaaactgccg cgcgactcga ctagaaccga acggctgatc tctaggatac
     2941 tcaccgcgcg acaacacgcc gacaatggtc tccacgcctc gtaggttcat gagggcgggt
     3001 ccgcccgagt ctccttcgag cgtgtgatgg tccaagtcac cgctctccaa ccatttgtcg
     3061 gaaaaggaag tcagcgagat cagagcctga cgcttgatgc cagcctcggg ctcgaagtca
     3121 tcgctgtcgc cgtagccaac cacacgcagg ggctgtccca ccagctccgc tcgcaacgcc
     3181 tcgcgattgt aagggagcgg agccactgga gccggctccg cgagcaaggc cacggctata
     3241 tcgtgccctt ccgaggaagc gtgcgagtca tattcagggt gcattctgaa atctacgata
     3301 tcgatccact tcgcgtgctc ccgatccatc tcgaaccgcg ctcgcttgac gtcgcgatta
     3361 tacacaacca agcaatgtcc tgccgtcaag atcacggtcg gcgcgattag cgtgccggtg
     3421 cacgtcgacg tgccgatcgt cccattttcg ttatccgtgg agccgacgag ataggccaca
     3481 gcggggtgtg ccgtgtcgac gtctccccca tatacctcgg gagttcccgt tcgctcgggg
     3541 ccgttcgcac ccgtctcggc cgaacaagcc gctgcggtta acgtcacgat cagcagccca
     3601 cctcgaatca ttcccaatcg accgaaatcc aagcgcatat cgactcctcg tgctggtttg
     3661 ggagcgcgcc aacgacgcgg ctctctcagc aatcggatcg tgtgggttca gctcgtttcg
     3721 ggtgatcgtc tgcgcttgct cggggccgcc gcgcgcgagc cgggagcacg cactccggta
     3781 caaagcagag aggcgagctt cacgctcgcc tctccttctc t
//
LOCUS       DASTKU010000184        22997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_35776,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000184 DASTKU010000000
VERSION     DASTKU010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 22997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22997
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35776"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VFQ61_07005"
     CDS             <1..1128
                     /locus_tag="VFQ61_07005"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HET9954233.1"
                     /translation="WSACLFAFVYLLYPPLHGANLYDFHYLPLGIFFLWLTVFLVESG
                     RTRWAVLSVIVTLSIREDVAAGLGILGAYLLLSGRRPLAGLVLAIVGVTYFVVMKLVI
                     MPLKLGDDSFVYMFKDLLPKGETGFSGVLKTVFSNPVFTLNSLLTQEKLLYVVQLAVP
                     LAFIPWRRPIGWLCTLPGFFFTLLSTGYSPLVQISFQYTANWISFIFIAGLANLAFVG
                     KAAFPGDTLGPIRRRSWMMTIAAMTLCTSYQYGAIMQLNTARGGFGPYIFGSTKQDQT
                     NYAKLKRLIRRVPPNAKIVSSENIVPHIANRADSYTMRIGAFDAEYLLGLLPANLDEA
                     RTMKQALDGAFGVIEIDMPFVLARRGYPKDRNSEALQHIHP"
     gene            complement(1468..2082)
                     /locus_tag="VFQ61_07010"
     CDS             complement(1468..2082)
                     /locus_tag="VFQ61_07010"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9954234.1"
                     /translation="MNLRNKILGHPFVFDYVRPLVTGGIDMSPAYERLECGPDSVVLD
                     IGCGTGDALKHLPTVKRYEGYDIDPAAIAIAQKRAAGRPHIEFRGRLCMPEDVALLQP
                     TEVALVGLLHHLSDAEVGPLLGMLKRSQRLKRVVSLDIVFLDGEPYGNFLARMDRGRY
                     CRTAPEYEQLVTSAGFNVVEQAIVRSHPTRGLVKYLVLAFEPML"
     gene            2240..3292
                     /locus_tag="VFQ61_07015"
     CDS             2240..3292
                     /locus_tag="VFQ61_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET9954235.1"
                     /translation="MASEDFAPLPLEVAKPARQRVSSARAFFSWFWLGAAIAQERARA
                     AGNERYQWFAKRARETNAAAHAVTEAREIPGAEAALPLACELFRQSAHWSLAALNSLG
                     STSPVDAASVGASARLWNSTDRDLLVYATGGPATFLEMQAVCTSPQPLAAIEQRSLIE
                     LGRLHLDLRLFSDRLLELVESPRRRLSTLKLKRALRLALPFLVLVVVAGGALAIADYR
                     EKKSDLTLNKPWTTSSNANVGGCPSPHQVCSDEPPYFFHTAEEATPWIEIDLGREESF
                     HGIRIHNRTDCCRERAVPLVVEVSNDRSKYSLVARQESNFRRWKQTFAPTKARYVRVR
                     ALKRTMLHLSRIMVLP"
     gene            3436..5622
                     /locus_tag="VFQ61_07020"
     CDS             3436..5622
                     /locus_tag="VFQ61_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9954236.1"
                     /translation="MTSPLAARSDFGLTGRPVSFDAGLRVLGLYDWSALGVTYRVEAT
                     LSDAPALALHVFGSAACPSEESVDELLLELAQHERSRPALNSGRLSDGRPGVLFPFES
                     AHLLADFIEQQTQPVDALLAVRFICSLCEALHGHGLAHGELSPRCILVRCTSSSLELA
                     RVLGWGFHPALQRLFEAGLPGALVEYRAPERFRGSAPKAQADVFALGSILARLLARSF
                     PTPPATVEQAEERALAADFEPGAGLLAQVPRVLQPILERALRADPQARFASAWELREA
                     LLTAAHELEPSQSALAELDFEPDWVSPAIMYPELGRLTRSQVPLANQSSIREVAPSSP
                     SPSWDWLHGPARETNATGVPRLGLGPARAALRHDLTCTQCGKVSPEHYKFCLGCGANL
                     PRELPQPHAPVPIVARAKDGAAADAPNLQESTPPRGVVPAGFQATPPAGTDPDSLSDW
                     TDLAWSAPVHEHVEQAFERASVRVMPTIPITAEPVAEADEPLILPEEIEPPSEDAPTS
                     FNAPAAEASGGVTEDGERESVLKPGAITALNRDGSTRAHFALIAGQPVLLGAQNGAPE
                     FAHDPFLSREHARFELRQGRLYAQDLDSLNGIYLKLPAHDPCEVFVGDYVRIGQQVLR
                     VDPLLEEHAHAGARLVRVSEHDTSEEGVLITERGTTCGRERGHLLFPEDGYVSSLHCR
                     LILTEGRFYLTDLGSSNGTFVRLRAETELEVGDLLLMGQQLLRIEA"
     gene            5662..7290
                     /locus_tag="VFQ61_07025"
     CDS             5662..7290
                     /locus_tag="VFQ61_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET9954237.1"
                     /translation="MTPLSEQAARDSSPEPGADEFEFDFVIVGSGFGGSVSALRLSEK
                     GYRVAVVEMGKRYRAQDFAKTSWDLRRFLWRPELGLYGILQLTLLRDVFVLHGAGVGG
                     GSLVYANTLLIPPETAFQNSGWVRPNFREALLPHYRTAQRMLGVVEAPHLYEGDEALL
                     EASRELGREAHFRKAQVGIFFGEPGKTVEDPYFEGKGPPRAGCVQCGACMVGCRHNAK
                     NTLDKNYLYLAEQLGAKVIPEHRVTLIEPLAGGGYQLSLERTTGIRKRRRRLRARNVV
                     VAAGVLGSLPLLMQCKARGALPNLSDKLGDRVRTNSEALLGVRARRDGPVLGGGIAIS
                     AGVDIDDQTHIEVVRYNEGSDFLGLLSTSLTDGEGGPWLRRWRWLKQTLTHPWSALKS
                     ANPVGWAKNTAILLVMQPVDNYLRLRWGRSWLPPFRRGLRSERSTSQRVPVYFPVAHQ
                     VARHMAKRLDAVPQSCNLEVLLNVPTTAHILGGCPIGRDAREGVIDEYCRVFGYEGLY
                     VVDGSVIPANLGVNPSLTITALAEYAMSHVPARR"
     gene            7611..8450
                     /locus_tag="VFQ61_07030"
     CDS             7611..8450
                     /locus_tag="VFQ61_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HET9954238.1"
                     /translation="MWRSIEQLVNQQVLRWTEAQRVSAQQSITRDTLPPVSGTAAGSG
                     PSDGLAQVDLEPTICISREFGTYAAELAREVAARIGFDVYDQELVDEISRQAHVRRKV
                     VESLDERVQHGLSAWVDELMELRSFTPSDYLRNLSQVVLTIGRHGRGILIGRGAHLIL
                     NPSHALRIRCYAPLDWRIQRVAEREGLSTAKATALTARVDAERVQFYREHFRTDFYDP
                     SNFDVLINASTTPLAARAELIVQAFEAKFAPTASPSLIPVSGVRAKPQLEPKESVIKA
                     VAG"
     gene            complement(8557..9294)
                     /locus_tag="VFQ61_07035"
     CDS             complement(8557..9294)
                     /locus_tag="VFQ61_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954239.1"
                     /translation="MRYLDRIRGAAGALAVLAVGFAALAVNPREASVTVAQAASGSGA
                     VDRTIVRFYTPDTGGVRRPHFVYERVLAFEARLEALSDPDRRGDVAYRSRHVNQALER
                     HVAETVLASLHIDPEPRAGELLSQTQAARARLAERVGGEEALEQARAAEGIGERDLLR
                     ILQRQARASLYLDRMVAPMLEPTEHELRALHKTRKNPFAALPYERIAPGLRRWYVSQR
                     LATALQAYYQNARSRIQLTLLGDLPER"
     gene            complement(9294..10106)
                     /locus_tag="VFQ61_07040"
     CDS             complement(9294..10106)
                     /locus_tag="VFQ61_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HET9954240.1"
                     /translation="MLDPDNPKSEVSSPPLRDVPIEWEALEDAFENNAPEVHSYLHLS
                     TGEVLRVVDGIADPDMHARIASDSTYMRVEPVSSREQYRWMERFIPMVEDAPLRDQLT
                     AAIDGKGAFRRFKDVLMGYAAERERWFTFRSERLRVFMEAWLTTHGLHAVGRPTWTQQ
                     EAAPRPRENVNISQEHLTARRSRSAEALRKQLKELSDSLGARDLEKVTAFAEFVKARR
                     AARVFAHRHEGDSGHEPESEPSVPIHEAPVGEAHSNDEEPSPSSRKRAFALK"
     gene            complement(10117..11619)
                     /gene="glpK"
                     /locus_tag="VFQ61_07045"
     CDS             complement(10117..11619)
                     /gene="glpK"
                     /locus_tag="VFQ61_07045"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET9954241.1"
                     /translation="MARYLLAIDQGTTGSTALVMSTEGQTLASANREFPQYFPRPSWV
                     EHEPDEIYESVLGSVSEALRLAGVHATAVGAIGITNQRETTLLWERESDRPVHRAIVW
                     QDRRTAQRCQELIAQGLEPRIRSKTGLVLDPYFSGTKLEWLLDHVAGGRERAERGELA
                     FGTVDSYLVHRLCGGARNSAPHVTDVTNASRTLLMDLLTRTWDAELCQLFRIPSAVLP
                     RIVPSAGVVGATHGVPGLPDGIPIAGLAGDQHAALFGQACFDAGDAKCTYGTGAFLLT
                     NTGPRAVPSRHGLLTTLAWQIGDEVCYALEGSCFVAGAAVQWLRDGLGLIATAPEIEP
                     LARQVASAEGVVFVPALSGLGAPHWDAEARGLITGITRGTTRAHLAYATLEGIAFQVC
                     EVLSAMAEDLGESIRRLRVDGGAAANDLLMELQADLAQLSVERPNELESTARGAAMLA
                     GVGSGLFADGRQAAKLVRIEKAFEVKMGEVERQGRLNTWQDAVRRARSKA"
     gene            11845..13716
                     /locus_tag="VFQ61_07050"
     CDS             11845..13716
                     /locus_tag="VFQ61_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9954242.1"
                     /translation="MLRLAANGSLPDLYMARVLLSTPDGQQVVELRDHNSLGRHPSNS
                     IQLLDKIVSKEHCTIERRGAGFILKDLGSLNGTYVNGERVNGEQELRHGDDIALGSTR
                     GRFDDGSGRAVAPPAPYGSPIGPLGAAPVWPPGQPAQPAAAQPGYAPSAAQPAPAAPH
                     GTSVIPVTAPNPAPAPPAYSTMAMQQVPPQQIPRVDVNDRARAIGNQMAATHKGFMPY
                     DQVVGNPQQLAADYERLRLSHELSREIALERDLPTLLNKILITIFKFVRADRGVIFLK
                     GQDGQLVPGATLRRDGTDSPIPVSSTIMNHVIQERATVLTHDAAMDFAASKGKSMILN
                     RISSAIVAPLLHNDEILGVLWLDSETLAQFQPKDMEIVTAIAAQAAMFIEINILGKKI
                     EQEIVNRERFSRLLSPNVAERVLSGELDVKQGGQLVQECTVFNSDIRGFTPMCEGAQP
                     ETLVEMLNEYFELMVETLFKYEGTLDKFMGDGIMALWGAPVMHPDDPLRAVCCALEQM
                     EVLGRFNRRRIELGRPPLAIGIGIHTGPLVAGYIGSSRALSYTVIGDTANTSARLCGV
                     AQSGQILVSETTLSRLAGRFDVEELPPTQLKGKERPFRVFNVRRLRPSVQVPLAAGG"
     gene            complement(13794..15251)
                     /locus_tag="VFQ61_07055"
     CDS             complement(13794..15251)
                     /locus_tag="VFQ61_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954243.1"
                     /translation="MHSRAVVWTRALLSIGLLLAFAWSKWSARPDKTPILTGDGRSWA
                     LVAESSIASREFWFGSRPFTLPLIYKLAEMSEERVVLFQSVLGVVAWGFFATCIAKIL
                     PKGFRTLVPLSLVLLTGLSLRVLEWDSVIRSESLAISFLVASVGCAVRVLLAEGSSRS
                     GWVWVGGALLFGVLSAFARDANGYFFLIFPALVIAPWLLLSQPSIRARVRRQGLAMGL
                     GMVLIAVGGSINTRESGRYAFPLINVVLRRVLKNPEKLAYFRDELGLPVSRALKARAG
                     KFASADHRYVYRSPELAAFRNWIYERGYAGYQRYLLSHPGLTWAEAADYMPRLVEWKA
                     GRLASKSANPTSTKVDAALNELCPPKLAWSVVAGLVASGAFMQLLRMPRARLLGLLTA
                     AFAILAGLQCYVVFHGDDMEIGRHGVLVPILLNLGCVLWICAWWFVLSEGLIALFARG
                     GGHSARRWMLPSWRLPDLVRRKARAAALAERKSVS"
     gene            complement(15498..18221)
                     /locus_tag="VFQ61_07060"
     CDS             complement(15498..18221)
                     /locus_tag="VFQ61_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HET9954244.1"
                     /translation="MTARLPDLSRTEPASPVSAPSSGPLARFDADLTQDLRFGRAELV
                     LTRTELRYREEGQAESRIPLRSNLEIQRREHGGLSELSVWDGETRLLRARFTLERARA
                     AHAFVDAFETAVAHHHKSHSSRPSIDEEEAEETLEAEEPSLASLRMPLLRLLAFARPH
                     LGAVGLGLALTITSTGVGLVPPYLTMPLVDRVLVPGQAGRLQAEQAYGQVALYLGGLG
                     LAALFAWGLHWAQGAVLARATEQIAADLRNRAFAHLQRLSLEYFGGKRTGDLIARIST
                     DTEHLCSFLSDSLIDFITDVLMIVGTCVVLLTLDPILALAAIVSFPPIAWLIVRVRGR
                     LTQGFLRGGRVWSAMTNILADTIPGIRVVKAFSQERREIARFAQANERIVEVNSRVNA
                     LWTFFWPLVALLNQIGLLVVWAVGAYQVLHQRVTVGILTAFIAYIGRFYVRVESMSRM
                     LTATQRAAAGAQRLFEILDRVPSVLEPKQPAALRTPQGEVRFERVSFRYGSRLVLDDL
                     TFRVAPGQMLGIVGHTGSGKSTVANLICRFYDVASGSVLFDGIDIRKLALDDYRQHIG
                     IVLQDAFLFFGTIAENIAYGLPGAGIERVVSAARAARAHEFILRLPEGYDSIVGERGQ
                     ALSGGERQRIAIARAILVDPKILILDEATSAVDVQTEREIQRALDNVVAGRTTIAIAH
                     RLSTLRKADLLLVLKEGRLAEMGTHAELMARDGEYARLQREKAARVASEVELDAAPDA
                     DEEEPESVVAQLDPRTLQLELDPTGQLWARSAERGPRIPVSARRAFPLSAPDFMICLV
                     DDRGHERVCFEDISQLPSAMRDTLLRALADCEFVPRITSIHEVRQRGTRFEWTTSTDR
                     GARTFLIEQEDHLRKLPDGRHLVLDSHGMRYMLPASEQLDAHSRKLLSRFA"
     gene            18490..21090
                     /gene="cphA"
                     /locus_tag="VFQ61_07065"
     CDS             18490..21090
                     /gene="cphA"
                     /locus_tag="VFQ61_07065"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET9954245.1"
                     /translation="MEIRKINVLVGPNIWANFPVLEAWVDLGHFEDFPSNKLPGFNDR
                     IMAWLPSMVEHRCSVGERGGFFKRMRDGTWLGHMLEHVSLELHSLSHSPVGYGRARET
                     SERGVYKVVIECDDPEFGRACLLTARELILAAVDDRPFDLSGELKKLREAADQYCYGP
                     STEAIVEAARARGIPTIRLTEHNLVQLGYGKSQRRIWTAETDSTGAIAEAIAQDKDLT
                     RKLLSGVGIPVPAGRTVTSAEDAWAAAEDLGLPVVVKPQDANHGRGVSIRIEDREGVQ
                     TAYQVALKEGSGVVVERFVPGTHYRVLVVGERAIAASGGTANQLLGDGISTVERLVEL
                     ANQNPERSDDSAQPLAPFVLDTIALELLRRQGLEPKTVPEAGRRVLIRYNGDLTVDET
                     ARMHPEVAASCVLAAQTIGLDVAGIDVIAEDIGRPLERQGGAIIEVNSSPGLIMHLKP
                     LTGKPQPVGEAIVNHLFKATDEGRVPLVAVTGTNGKTPVAGALAHMLAAAGRSVGRAD
                     STGVHVRGRQLAKENGCTAQSARRVLINPFVDAIVLEVSEADVLDQGLAFDHCDVAVI
                     TNIACGDHLGKRYVDELTTIEKAIRAPLDTVRAKGVGVLNASDPAVVAMADKCRGEVM
                     WFSRSPDQIEAHLSHGGRGVTVENDQIVLRTAQRREYVLPTSRLSCAVLGLPDMLLDN
                     ALTSVAAGWALGLNLTALRAGLEQQLSPAEGVLYEHKGRLIIVSRARNAAALGGWLQV
                     LQTRFADRRWRCVVEPPRDWRIQDAQALGKTLSSAFARVQAIYTHENQALAEALETET
                     QDPASRPSGPVTRFCEAVNQMLEASEATDSLFAQPASARDYDALISLLENKGAVARRV
                     SGLVSVENCR"
     gene            21087..>22997
                     /gene="cphA"
                     /locus_tag="VFQ61_07070"
     CDS             21087..>22997
                     /gene="cphA"
                     /locus_tag="VFQ61_07070"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR02068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET9954246.1"
                     /translation="MKLDSIRALRGPNLWSDETVLEASLLGDLSGFGGETLARLRAAL
                     PQVVADRLAETTALEARLEGDIAEQTSLHWARLVGALACSLQYATGADVGYHHAQEWG
                     SPPCSSKQPGSSKQPRTEHRIVVEYAEEEIGRKALAMAVEIVLAARAGTSLDLEAQLT
                     ELRHAYENERLGPSTGSIVRAALDRGIPARRLTSGSLVMLGHGARQRRIWAAETDRTS
                     AIAEAIAQDKELTKSLLRAAGVPVPEGRPVESPEQAWEAAQEVGLPVVVKPRNGNQGR
                     GVSVRLSSEEAVKLGYELARKQSSEIIVEKHVEGADFRLLVIGGKLVAAARREPPKVI
                     GDGRNDIAELVRVENLDPRRGDDHSTSLSKLRLDEIGLEMLAEQGLAPESVPAAGQVV
                     VLRRNANLSTGGTAADVTDEVHPEVAKRVADAAAMIGLDIAGIDVMAPRLDIPLEKSG
                     GAIVEVNAAPGLRMHLEPSSGKPRNVGSAIIESMFGRGDDARIPIVAITGTNGKTTTT
                     RCVAHLLKQHGLRVGMTCTDGIYVEGRRIDTGDCSGPKSARVVLGHPRVDAAVLETAR
                     GGMLREGLGFDWCDVAIVTNVGEGDHLGLNGIYTVEDLARVKEIPVRRVSPRGAAVLN
                     ADDPLVVAMAGAC"
BASE COUNT         4352 a         7250 c         7256 g         4139 t
ORIGIN      
        1 tggtctgcgt gcctgttcgc cttcgtgtat ttgctttacc ctccgctcca cggcgcgaac
       61 ctctacgact ttcattatct gccactcggc atcttctttc tctggctcac cgtgttcctg
      121 gtagagtcgg ggcgcacgcg ctgggccgta ctcagcgtca tcgtcacgtt gtcgattcgt
      181 gaagacgtgg cggcggggct cgggattctg ggggcctatt tgctgctctc tggacggcgc
      241 cccttggcgg ggttggtgct ggcgatcgtt ggggtcactt acttcgtggt catgaagttg
      301 gtgatcatgc cgctgaagct aggtgacgac tcgttcgtct acatgttcaa agacctgctc
      361 cccaaaggag aaacaggctt cagtggtgtt ctcaagacgg tgttttcgaa ccccgtcttc
      421 acgctcaata gcctactcac ccaggagaag ctgctgtacg tggtgcagct cgcggtacca
      481 ctggcgttca ttccgtggcg tcgaccgatc ggctggctgt gcaccctccc ggggttcttc
      541 ttcacgttgc tttcgacggg ctactcccct ctcgtccaga tctcgtttca gtacaccgcc
      601 aattggatta gcttcatctt cattgcgggc ctcgcgaacc tggccttcgt cgggaaggca
      661 gcttttccgg gagacaccct cggccccatc cggcggcgga gctggatgat gacgattgcg
      721 gcgatgacgc tgtgcacgag ctatcaatac ggcgccatca tgcagctgaa cacggcgcga
      781 ggcggattcg ggccgtacat cttcggctcc acgaagcaag atcagaccaa ctatgcgaag
      841 ttgaaaaggc tgatccgccg cgtgccaccg aacgcaaaga tcgtgagctc cgagaacatc
      901 gtgcctcaca tcgcgaatcg cgcagactcg tacaccatgc ggatcggcgc tttcgatgca
      961 gagtacttgt tgggcttgct cccggccaac ttggacgaag ctcgcaccat gaagcaggca
     1021 ctcgacggcg ccttcggcgt aatcgaaatc gacatgccct tcgtgctcgc ccggcggggt
     1081 tatccgaaag atcgcaatag cgaggcgctg caacacattc atccctgaga gtgaagcggt
     1141 acttaccggc gccggtcacg gatgccgtcg agcacaggtt gagtgagcag accggaccgc
     1201 gcgcgctagc tcgcttcagg gtgagatcgg tgtacgctgg cagctacggt gagcgtgaat
     1261 ttgcgatttc gttgttcgat gaggaaacgc ctgacgggat gtttttcgac acgtccgtct
     1321 cacagcactt cgtctgggcg acacctatat cctttttctc aagagcaaac gggttctcct
     1381 cgtcgcttgc tgcggagacc ccgcgcatag cgcaaacgag cgccggagat gttagcggat
     1441 caaagcggcc taactccgca cggtgctcta aagcataggc tcgaacgcga gcaccagata
     1501 cttgaccaag ccgcgcgtcg ggtggctgcg tacgattgct tgctcgacta cgttgaagcc
     1561 agcggaggtg acgagctgtt cgtattccgg tgcagtccgg cagtagcgtc cgcggtccat
     1621 acgagccaag aagttgccgt aaggctcgcc gtccagaaac acgatatcca agctgacgac
     1681 ccgcttgagc ctttgcgaac gcttcaacat gccgagcagt ggcccgactt ccgcgtcgga
     1741 gaggtggtga agcagaccca cgagcgccac ctcggtaggc tgcagcagcg cgacgtcctc
     1801 gggcatgcag agccgcccac gaaattcgat gtggggacgc cccgccgcgc gtttttgtgc
     1861 gatggcgatg gcggcgggat cgatgtcgta accttcgtaa cgcttcaccg ttggcaggtg
     1921 cttcagagcg tcacccgtgc cgcatccgat gtccaaaacc accgaatcgg ggccgcactc
     1981 caagcgctcg tacgccgggg acatgtcgat gccgcccgta accagcgggc gaacgtaatc
     2041 aaagacgaaa gggtgaccga ggatcttgtt ccgcaagttc atggggctga agcggcctgg
     2101 agagcgcggc ggagtttgcc atgagcaatt aggactagct accgttcgct atgacacctc
     2161 ggccgactga cctgagcaac ctgtgatgtg acgttcgttt tctgataggc ctggggtata
     2221 aggtgccgcg aacgaatcaa tggcttcgga agatttcgct cccctgcccc tcgaagttgc
     2281 caagccggct cgccaacggg tgtctagcgc tcgtgccttc ttcagctggt tctggctggg
     2341 tgctgccatc gcgcaagaac gagcgagagc ggcgggtaac gagcgatacc aatggttcgc
     2401 gaagcgcgca cgagaaacga acgctgccgc acatgccgta accgaagccc gggaaatccc
     2461 cggagccgaa gcggcgctcc cgcttgcttg cgagttgttc cgacaatcgg cgcattggtc
     2521 cttggccgcg ttgaatagtt tgggttcgac gagcccggtc gacgccgcga gcgtcggcgc
     2581 gagtgcgcgt ttgtggaaca gcaccgatcg agaccttttg gtttacgcga ccgggggccc
     2641 cgcaactttc ctagagatgc aggcggtctg cacgagccct caaccactcg ctgcgatcga
     2701 gcagcgctcg ctcatcgagc tcggccgtct gcaccttgat ttgcggcttt tctcggatcg
     2761 actgttggag ttggtcgagt caccgcgccg gcggctcagc acgctaaagc tcaaacgcgc
     2821 tctgcgtttg gccttgccat tcttggtatt ggtggtcgtc gcggggggtg cactcgccat
     2881 cgcagactac cgcgagaaga agtccgattt gacgctcaac aagccgtgga cgacgagctc
     2941 taacgcaaac gtggggggtt gcccatcgcc acaccaagtt tgctcggatg aaccgcccta
     3001 cttctttcat acggcggaag aagcgacgcc gtggatcgag atcgacctgg gcagagagga
     3061 atcctttcac ggcatccgaa ttcacaaccg caccgactgc tgccgggaac gggctgttcc
     3121 gctcgtggtc gaggtttcca acgatagaag taagtactcc ctcgtcgccc gccaggagtc
     3181 caacttccgc cgctggaagc agaccttcgc cccgaccaag gctcgttacg tccgagttcg
     3241 ggcactaaaa cggacgatgc tgcatctgtc ccggatcatg gtgctgccct gagctgaccg
     3301 gcgggtcggc gtcctccccg gtgttggcgg agtgcgacat gtgggagtcg aacgggttcg
     3361 gttgccttag ttagtgggtg gtagtgcgtt tttgggggat acggcgcctt ttcgtcgata
     3421 acataggcgc gtcgcatgac ctcacccttg gccgcacgtt ccgacttcgg gctaacgggg
     3481 cgtcccgtgt ccttcgatgc gggtctgcga gttctgggtc tttacgattg gagcgcgctg
     3541 ggtgtcacct atcgggtgga agcgactctg agcgatgcgc cggcgttggc attacacgtg
     3601 ttcggtagcg cggcctgtcc aagtgaagag tcggtcgatg agctgttgct cgagctggcc
     3661 caacacgaac gctcgcggcc cgcgttgaat agcggacgac tgagtgatgg tcgtcccgga
     3721 gtgctgtttc cattcgagag cgctcatttg ctggctgatt tcatcgagca gcagacacag
     3781 ccggtggatg cgctgctcgc ggtgcgcttc atctgttcgc tctgtgaggc gctgcatggg
     3841 cacggcttgg cacacgggga gctttccccg cgctgcatcc tcgtgcgctg cacctcaagt
     3901 tcactcgagc ttgcccgagt gctgggctgg ggttttcatc cggcgctcca gcgtctattc
     3961 gaagccgggc tgccaggagc gctcgtggag taccgcgcgc ccgaacgctt ccggggttcg
     4021 gcgccgaagg cgcaggccga tgtcttcgcg ctcggcagca ttctggcccg gcttttggcc
     4081 cgctctttcc cgacgccgcc cgccacggtc gagcaggcgg aagaacgtgc cttggcggct
     4141 gatttcgagc ctggtgctgg cttgctcgcg caagttccac gagtgctgca gccaatcttg
     4201 gagcgggcgc tgcgcgcgga tccgcaagct cgctttgcaa gtgcttggga actccgcgag
     4261 gccttgttga cggccgctca cgagctggag cctagtcaat cggccctggc tgagctggat
     4321 ttcgaacccg actgggtgag cccggcgatc atgtacccag agctaggccg gctcacgcgc
     4381 tctcaagtgc ctctggcaaa ccaaagctcg atccgagagg tggcgccttc ctcgccctcg
     4441 cccagctggg attggttaca cggccctgct cgagagacga acgcaacggg agtgcctcgg
     4501 ttgggactcg gccccgcccg tgcagcgctc cggcatgacc tgacctgcac tcagtgcgga
     4561 aaagtcagtc cggagcatta caagttctgc ttgggatgcg gcgcgaatct cccgcgcgag
     4621 ttgccgcagc cacacgcccc agtgccgatc gttgcgcgag ccaaggatgg cgccgcggcg
     4681 gacgcgccga acctgcaaga atccacgccc ccccgaggcg tggtgccagc cggatttcag
     4741 gccacgccgc ccgcggggac ggatccggac tcgctctcgg attggaccga tttggcctgg
     4801 tctgcaccgg ttcacgagca tgtggaacag gcgttcgagc gcgcgtccgt gcgcgtgatg
     4861 cccacgattc cgatcaccgc cgagcccgtc gcggaagcgg acgaaccact aattctgccg
     4921 gaagagatcg agcctccatc ggaggacgcc ccgacgtcgt tcaacgcgcc ggcagccgaa
     4981 gcatcgggtg gagtgaccga ggatggcgaa cgcgaaagtg tgctcaagcc cggggcaatc
     5041 acagcgctca accgggacgg ctccacgcgc gcgcacttcg cgctcatcgc cgggcagcca
     5101 gtgctcttgg gcgctcagaa cggtgcaccc gagtttgccc acgacccctt cctatctcgc
     5161 gaacacgcgc gcttcgagct gcgccaaggg cggctctacg cccaggactt ggattcactg
     5221 aacggcatct acctgaagct gcccgctcac gacccgtgtg aggtattcgt gggcgactac
     5281 gtccgcatcg ggcagcaggt gctgcgcgtc gatccgctac tcgaggagca cgctcacgcc
     5341 ggggctcgcc tggtgcgggt ctccgagcac gataccagcg aggaaggcgt tctgattacc
     5401 gagcgcggca cgacgtgcgg ccgcgaacga ggccaccttc ttttccccga ggacggctat
     5461 gtgtcgagcc tgcattgccg gctcattctc acggaaggcc gtttctacct cactgacctc
     5521 ggcagctcga atggcacgtt cgtccggcta cgtgcggaaa ccgagctcga agtgggtgat
     5581 ctgctgttga tggggcagca gctgctgcgc atcgaagcct gacgtggcgc tagcggcttc
     5641 gagcccctac acttcctgga gttgacgccc ctctcggagc aagctgcacg cgactcgagt
     5701 ccggaacctg gagcggacga gttcgagttc gacttcgtca tcgttggctc tggcttcgga
     5761 ggctccgttt cggccttgcg cttgtccgag aagggttatc gcgtcgccgt ggtcgagatg
     5821 ggcaagcgct atcgggccca ggatttcgcg aaaacgagct gggatttgcg ccgcttcctg
     5881 tggcgtccgg agctcgggtt gtacgggatt ttgcagctca cgttattgcg cgatgtgttc
     5941 gtgttgcacg gcgcgggtgt cggcggtggc agcctggtct acgcgaacac gcttttgatc
     6001 ccgcccgaga ccgcgttcca aaattcgggt tgggtgcggc ccaattttag agaggcgctg
     6061 ctgccgcatt accgaacggc tcaacgcatg ctcggcgtgg tggaggcgcc tcacctctac
     6121 gagggggacg aggccctgct ggaggcgagc cgagaactgg ggcgagaggc acatttccgc
     6181 aaagcccaag tcggaatttt cttcggtgag ccgggcaaga ccgtcgaaga cccctacttc
     6241 gaaggaaagg ggccccctcg agctggctgc gtgcaatgcg gagcatgcat ggtgggctgc
     6301 cgccacaacg ccaagaacac tctcgacaag aactacctct acttggccga acagctcgga
     6361 gccaaggtga ttccagaaca ccgggtcacg ttgatcgagc cgctcgcggg tggcggatac
     6421 cagctcagcc tcgaacgcac gacgggcatc cgcaagcgac gacggcgctt gcgggcccgc
     6481 aacgtcgtcg tcgcagcggg cgtactcggt tcgctgccgc tactcatgca gtgtaaggcg
     6541 cgcggcgcgc tgcccaacct atccgacaag ctgggagacc gagtgcgcac caatagtgag
     6601 gcgctgctcg gcgtgcgtgc gcggcgcgac ggacccgtgc tgggcggagg tattgccatc
     6661 tcggccggcg tagacatcga cgatcagact catatcgagg tcgtgcgtta caacgaagga
     6721 tccgatttct tgggtctgct cagcacgagt ctgaccgacg gagagggcgg cccgtggctg
     6781 cgccgttggc gttggctcaa acaaacactc actcaccctt ggagcgcgct gaagagtgcg
     6841 aatcccgtcg gttgggccaa gaacactgcc atcttgctcg tgatgcagcc ggtcgacaat
     6901 tacctgcgtc tacgctgggg ccgcagttgg ttaccgcctt ttcgccgcgg cctacgttcg
     6961 gagcggtcga cctcgcagcg ggtacccgtg tattttccgg tggcgcatca ggtggcgcgc
     7021 cacatggcca agcgccttga cgcagtgccg caaagttgca atctcgaggt gcttttgaat
     7081 gtgcctacga cggcccacat cctcggtggc tgtccgattg ggcgagatgc acgagagggc
     7141 gtgatcgacg aatactgccg tgtcttcgga tacgagggct tgtacgtcgt agatggctcc
     7201 gtcataccgg cgaacctcgg agtcaatccg agcctcacca tcacggcttt ggctgaatac
     7261 gcgatgagtc acgtgccagc ccgccgctga agttcgcgcg tgattcctgc aagacgaggg
     7321 cagaagcgat gcgcggagcc ggcgggagag caccgagatc cgcgcccgga ctcgtgtgtt
     7381 aatcagtctt ctttcggggt tcttggaaaa ctcgaagcgg ggcggtctct gcacaccaac
     7441 tggcgaattg acctgcaagt gatctccatc attggtattc ttgcacccga gtaatcgctt
     7501 cgagcgtcgg ttcggcaaag atgcaggctt cgcgatcgcg cctgcacgaa aacactacgg
     7561 ccccggcgcg gtgcttatca actcgtgtgc aggtacgaat ggaggcgccc atgtggcgaa
     7621 gcatcgagca actcgtcaat cagcaggtgt tgcgctggac ggaagcacag cgcgtgtccg
     7681 cccaacaatc cataacacgc gatacattgc cacccgtctc gggcaccgcg gctggaagcg
     7741 gtcccagcga tggcttggcc caagtggatc tcgaacccac gatctgcatc tctcgcgaat
     7801 ttggcacgta tgccgctgaa ctcgcgcgtg aagtggccgc gcggattggc ttcgacgtct
     7861 acgatcaaga gctcgtggac gagatttccc gtcaagccca cgtgcgccgc aaggtggtgg
     7921 aatcgttgga cgagcgcgtc cagcacgggc tcagtgcgtg ggtagatgag ctcatggagc
     7981 tgcggagttt cacaccgagc gattacctac gtaacctatc gcaggtggtc ttgacgatcg
     8041 gtcggcacgg gcgtggcatc ctgattggtc gcggggcgca cctgatcttg aatccctccc
     8101 atgcgttacg aattcgttgc tatgcgccgc tcgattggcg aatccaacga gtcgccgagc
     8161 gggaggggtt gtctaccgcc aaggccaccg cgctcacggc gcgcgtcgat gcggagcgag
     8221 tgcagtttta ccgagagcac ttccgaaccg atttctacga tccatcgaac ttcgatgtgc
     8281 tgatcaacgc cagcactacg ccactggcag cccgcgcgga gctgatcgtg caagccttcg
     8341 aggcgaagtt tgcgccgacg gcctcgccgt cgctaatccc ggtctcgggc gtgcgcgcca
     8401 aaccccagct cgagccaaaa gaatccgtga tcaaagccgt agcgggctag tcggggtcta
     8461 ggacggcgcg ctgacggctc gcggcgcgcc cccaatgctc gggttagccc ggcactcgga
     8521 tgccccctcg cacccgagcc ctcgcactcg ggtagctcag cgctccggca gatccccgag
     8581 taacgtcagc tggatgcggc tgcgcgcgtt ttggtaatag gcctgaagtg cggtggccaa
     8641 gcgttggctg acataccaac gccgcaagcc gggcgcgatg cgctcgtatg gcagagcggc
     8701 aaacgggttc ttgcgcgtct tgtgcagcgc cctcagctca tgctccgtgg gctcgagcat
     8761 gggcgcgacc atgcggtcga ggtagaggct cgctcgcgcc tgccgctgca agatgcgcaa
     8821 gagatcccgc tcgccgatgc cctccgcggc gcgcgcctgc tccagcgctt cttcgccgcc
     8881 cacgcgctcc gccaggcgag cgcgtgccgc ttgcgtctgc gacagcaatt cgccggcgcg
     8941 aggctctggg tcgatgtgta aagaggcgag caccgtctcc gcaacgtgac gctcgagcgc
     9001 ctggttgacg tggcggctcc ggtaggcaac atcgccgcgt cgatcgggat cactcagcgc
     9061 ttctaaacgc gcctcgaacg ccagaacacg ctcgtagacg aagtgcgggc gccgcactcc
     9121 gccagtgtcc ggcgtgtaga accgcacgat cgtgcggtcg acggcgcccg agcccgaggc
     9181 ggcctgcgca accgtaacgg aggcctcgcg tgggttcaca gccaacgccg cgaacccaac
     9241 cgcgagtacc gccagcgcgc cagccgcgcc gcgtatccga tcgaggtaac gcactacttc
     9301 aaagcaaagg cgcgtttgcg cgaggaaggt gaaggttcct cgtcattcga gtgcgcctca
     9361 ccgaccgggg cctcgtggat cggcacgctc ggttccgact ccggctcatg gccagaatct
     9421 ccttcatgtc gatgcgcgaa cacccgtgcg gcacgccgtg ctttgacgaa ctccgcgaac
     9481 gcagtgactt tctccagatc gcgcgccccg agcgaatccg agagctcttt gagctgcttg
     9541 cgcaaagcct ccgcactcct gctccggcgc gcggtcaaat gctcctgtga gatattgacg
     9601 ttttcgcgcg gacgcggcgc ggcttcctgc tgtgtccagg tgggccgccc cactgcgtgc
     9661 aggccgtgag tggtgagcca ggcttccatg aacacgcgca gccgttcact gcgaaaggtg
     9721 aaccagcgtt cccgctccgc ggcgtagccc atcagcacgt ctttgaagcg acgaaatgcc
     9781 cctttgccgt cgatggccgc cgtgagttga tcgcgcagcg gggcatcttc caccatcgga
     9841 atgaagcgct ccatccagcg gtactgctcc cgcgaggata cgggctcgac gcgcatgtac
     9901 gttgaatccg atgcaatgcg cgcgtgcata tcgggatcgg cgatgccatc tacgacgcgc
     9961 agcacctcac cggtgctcaa gtgcaagtag ctgtgcactt ccggcgcatt gttctcgaac
    10021 gcatcttcga gggcctccca ctcgatgggc acgtcacgca aaggcggaga ggaaacctcc
    10081 gactttggat tgtccggatc caacatgttt tctcctctaa gccttgctcc gagcgcggcg
    10141 tacggcgtct tgccaggtat tcagccttcc ttggcgttct acctcgccca ttttgacctc
    10201 gaacgccttc tcgatcctga ccaatttcgc ggcttgccgt ccgtcggcaa aaaggcccga
    10261 gccaacgccc gcgagcatcg cggcgcctcg ggccgtggac tccagctcat tcggcctttc
    10321 gaccgagagc tgagccagat ctgcttgaag ttccatcaga agatcgttcg ctgccgcgcc
    10381 cccgtcaacc cgcaatcttc ggattgactc tcccaggtcc tccgccatcg cagaaagaac
    10441 ctcacacacc tgaaacgcaa taccctcgag cgtcgcgtac gcgaggtgcg cgcgagtcgt
    10501 acctcgcgtg atgccggtga tcaaaccccg ggcctcggcg tcccagtgcg gcgcacccaa
    10561 accggaaagt gcgggtacga atacaactcc ctccgcagat gcgacttggc gcgccaaggg
    10621 ttcgatttcg ggcgcagtcg cgatcaagcc cagaccgtcg cgcagccatt gcaccgccgc
    10681 tccggctacg aagcagctac cttcgagcgc gtaacagact tcgtcgccaa tctgccaagc
    10741 caaagtcgtc agcagaccgt gacggctcgg aaccgcgcga ggcccggtgt tcgtcagcaa
    10801 aaacgcgccc gtgccgtagg tgcatttggc gtcgcctgcg tcgaaacaag cctggccgaa
    10861 gagtgcagcg tgctgatccc cggccaagcc agcgattgga atcccgtcag gcagccccgg
    10921 aaccccgtgc gtcgcgccaa ctactccggc gctcggaacg atgcgaggca acaccgcgct
    10981 cggaatccta aacagctggc agagctctgc atcccaagtg cgggtcaaaa gatccatcaa
    11041 caacgtgcgg gacgcattgg tcacgtcggt gacgtgcggc gccgaattcc ttgcaccacc
    11101 acacaagcga tgaacgaggt agctgtcgac cgtaccgaac gcgagctctc cccgctccgc
    11161 gcgctcacgg ccaccggcaa catggtcgag cagccattcg agcttggtgc cggaaaagta
    11221 cgggtctaag accagcccgg tcttgctccg gattcggggc tccaagcctt gtgcgatcag
    11281 ctcttggcaa cgctgtgcgg tccggcggtc ctgccaaacg atggcccgat gaaccggtcg
    11341 gtcgctttct cgctcccaga gcagcgtggt ttctctctga ttcgtgatcc caatcgcgcc
    11401 caccgccgtg gcgtgaactc cagccaagcg cagcgcttcg ctgacagagc cgagtacgct
    11461 ctcatagatc tcgtccggct cgtgctccac ccacgaaggc cgcgggaagt actgcggaaa
    11521 ttcgcgattg gcggaggcga gagtttgccc ctccgtgctc attacgagcg cggtcgagcc
    11581 ggtggtcccc tgatcgatgg ccagtaggta acgcgccacg ggagcggcag gatatcagaa
    11641 cttcgtgggg cgcaggcgaa aggtctgggg ctgaccgtat tggcgcgagg ccatgttgcc
    11701 gctgggccaa cgctagctcg agtcccctcg gagccggcga tgtgggtggt gccagaagag
    11761 atgttactcg gggtgcgcgg tgggcctcgc agcccgtcgg ttcgagccgt cagcgagcgg
    11821 aaacgaacgg gaggcgttcg ggtcttgctg aggctcgctg cgaacggtag tctgcccgac
    11881 ctgtatatgg cgcgcgtgct cctttcgacc cccgacggcc aacaggtcgt ggagcttcgc
    11941 gaccacaact cgcttgggcg ccacccgagc aactcgatcc agctcctgga caagattgtg
    12001 tccaaggagc actgcacgat cgagcggcgc ggggcgggct tcattctgaa agacttgggc
    12061 agcctgaacg ggacctacgt caacggagag cgagtcaacg gagagcagga gctgcggcac
    12121 ggggatgaca tcgcgctcgg cagcacccgc gggcgcttcg atgacggctc gggtcgcgcg
    12181 gtagcgccac ccgcacccta cggctcgccc attgggcctc tgggtgccgc gcccgtgtgg
    12241 ccgccagggc agcccgcgca gcccgccgcg gctcagcctg gctacgcgcc ttccgccgct
    12301 caaccggcac ccgccgcgcc acacggaacc tccgtaatcc ccgtcacggc accgaaccca
    12361 gcccccgcgc cgcccgccta ctccacgatg gcgatgcagc aggtgccccc gcaacaaatt
    12421 ccgcgggtgg atgtgaatga ccgcgctcgc gccatcggca atcagatggc ggcgacgcac
    12481 aagggattca tgccgtacga ccaagtcgtc ggcaatcctc agcagctcgc cgcggactac
    12541 gagcgactgc gtctctcgca cgagctctcg cgagagattg cgctcgaacg agacctgccg
    12601 accctactca acaagatcct gatcacgatc ttcaagttcg tgcgcgccga tcggggcgta
    12661 atcttcttga agggccagga cgggcaactc gtaccgggcg ctacgctacg ccgcgacggc
    12721 actgattcac ccatccccgt ctcctcgacg atcatgaatc acgtgattca ggagcgggcc
    12781 acggtgctga cgcacgacgc agcgatggat ttcgcggcct cgaagggcaa gagcatgatc
    12841 ttgaatcgga tcagctcggc gatcgtggct ccgctcctac acaacgacga aattctgggc
    12901 gtactgtggc tcgacagcga gaccctggcc cagttccagc ccaaagacat ggagattgtg
    12961 acggccatcg cggcgcaggc cgcgatgttc atcgagatca acatcctggg taagaagatc
    13021 gagcaggaga tcgtcaatcg cgagcgcttc agtcgcttgc tctcgccgaa cgtcgccgag
    13081 cgcgtattga gcggcgagct cgacgtgaag cagggcgggc agctcgtgca ggagtgcacg
    13141 gtgttcaact cggacatccg tggcttcaca ccgatgtgcg aaggcgccca gccagaaacc
    13201 ttggtcgaaa tgctcaacga gtatttcgag ctgatggtcg aaaccttgtt caagtacgag
    13261 ggtactctgg acaagttcat gggggacggg atcatggcgc tctggggcgc acccgtgatg
    13321 cacccggacg atccgttgcg cgccgtgtgt tgcgccctgg agcagatgga agtgctcggg
    13381 cgcttcaatc gtcggcgcat cgagctcggt cgcccgccgt tggcgatcgg aattggaatc
    13441 cacaccgggc ctctggttgc cggttacatc ggaagctcgc gcgcgctcag ctacaccgtg
    13501 attggcgata cggccaacac gagcgctcgc ttgtgcggcg tcgcgcagtc cggccaaatc
    13561 ctggtgagcg agaccacgct ttcacgcctg gccggtcgct tcgacgtcga ggagctgcct
    13621 cccacccagc tcaagggcaa ggaacggccg ttccgcgtgt tcaatgttcg gcgcttgcgc
    13681 ccgagcgtgc aggttccgct ggccgctggc ggctgacgcg ccaagcaagt ctacggaacg
    13741 agcgccctcc aaaaagtcga gtgacgagcc ctggcgtgca cgcggcccgc gttctagcta
    13801 actgatttgc gctctgccaa cgcggcggct cgcgctttgc gccgcactag atcgggcaac
    13861 cgccagctcg gcaacatcca gcgacgcgcg gaatgcccgc cgccccgtgc gaacagcgca
    13921 atcaatcctt cggaaagtac gaaccaccaa gcacagatcc aaagcacaca ccccagattc
    13981 agcaggatcg ggactaacac tccgtgacga ccaatctcca tgtcgtcccc gtgaaacact
    14041 acgtagcact gcagacccgc caggatagcg aaggccgccg taagcaggcc caaaaggcgc
    14101 gcgcgaggca tgcgtaacag ctgcatgaac gcgccgctcg cgaccaatcc tgcaacgacc
    14161 gaccaggcta gcttcggtgg acacaactcg ttcagtgcgg catccacctt cgtcgatgtg
    14221 gggttcgcgc tcttggaggc aagacggccc gctttccatt cgacgagccg cggcatgtag
    14281 tccgcggctt cagcccaggt gagccccgga tgagagagca ggtagcgctg ataaccagcg
    14341 tacccacgtt catagatcca gttcctaaag gccgccaact cgggcgagcg ataaacatac
    14401 cggtggtcgg cagaggcaaa cttgcccgcc cgcgccttca acgcgcgaga aactggcaag
    14461 ccgagctcat cgcgaaagta cgctagcttt tccggattct tgagcacgcg gcgcagcact
    14521 acgttgatca aaggaaacgc gtagcgcccg ctctctcggg tgttgatcga gccgcccacg
    14581 gcgatgagca ccattcccaa acccatcgcc aggccttggc gccgcacgcg agcgcgaatc
    14641 gagggctgac tcagcagcaa ccaaggcgcg atcaccagcg cgggaaaaat caggaaaaag
    14701 taaccgttcg cgtctcgagc gaaggcgctg aggacaccga acaacagcgc tccgccgacc
    14761 caaacccagc cgcttcgtga gcttccctcc gctaacaaca cgcggaccgc gcagccaaca
    14821 cttgcgacta ggaacgagat agcgagcgac tccgagcgga tcaccgagtc ccactcgagc
    14881 acccgcaagc tgagccccgt cagtaagacg agcgacaacg gcacgagggt tctgaacccc
    14941 ttgggcaaga tcttcgctat gcacgtcgcg aagaaccccc aagcaacgac gcccagcacc
    15001 gactgaaaga gcaccactct ttcctcggac atctctgcca gtttgtagat caagggcagc
    15061 gtgaacggcc gtgagccaaa ccagaactca cggctggcaa tcgaggactc cgcaaccagc
    15121 gcccaacttc gaccgtcgcc ggtcaggatt ggagtcttgt cgggtcgcgc cgaccacttc
    15181 gaccatgcga aggcgagcag cagcccaatg ctcagcagcg cacgagtcca aaccacggcc
    15241 cgggagtgca cggcgtgggg cccgactctg cgggagtaga caaggcgaga gaacaagcct
    15301 gctcttgccg cacttttggc agggcctgcg gatttccacc cttggagctt agccaaaacc
    15361 tggtcgagct tacccacggg cgctagatag accgacttcg taccgcgcgc tagacctctc
    15421 cgcagagccc gtcgattgct cgacctctac ttctgctcga tctcgacttc gagttactgt
    15481 cgaacgagct atcgagctca agcgaagcgt gacagtagct tccggctatg cgcatcgagt
    15541 tgttccgagg cgggcagcat gtagcgcatg ccgtgactat cgagcacgag gtgacgtccg
    15601 tcgggcagct tccgaagatg gtcttcttgt tcgatcagga aggtccgcgc accgcggtcg
    15661 gtgctggtag tccactcgaa gcgagtcccc cgctgccgaa cctcgtggat gctggtgatg
    15721 cggggcacga actcacaatc tgcaagcgcc cggagcaaag tgtcgcgcat cgcagacggc
    15781 agctggctga tgtcttcgaa acacacccgc tcgtggccgc ggtcgtccac caggcaaatc
    15841 atgaagtcgg gggcgctcag agggaatgcg cgccgggcgc tcacgggaat acgcgggccg
    15901 cgctccgcac tcctggccca gagctgcccg gtcgggtcca gctcgagctg cagggtgcgc
    15961 ggatcgagct gggccaccac gctctcgggc tcttcctcat cggcgtcggg cgcggcatcg
    16021 agttcgacct ccgaggccac acgggccgcc ttctcgcgtt gcaatcgcgc atattcgccg
    16081 tcgcgcgcca tgagctcggc gtgtgtgccc atctcggcaa gccgcccttc cttgagcacg
    16141 agcagtagat cggccttacg cagcgtgctc agtcggtgag caatcgcgat cgtggtgcgc
    16201 cccgccacta cgttgtccag ggcgcgttgg atctctcgct ccgtctggac gtccaccgcg
    16261 gacgttgctt cgtccaagat caagatcttc gggtcgacca ggattgcccg cgcgatcgcg
    16321 atgcgctggc gttcgccgcc cgacaatgct tgcccacgct caccgacgat tgaatcgtag
    16381 ccctccggca ggcgcaggat gaactcgtga gcgcgcgcgg ctcgcgccgc agagacgacg
    16441 cgctcgatgc ccgcccccgg caggccataa gcgatgtttt cggcaatggt tccgaagaac
    16501 aaaaacgcat cctggagcac gatcccgatg tgttggcgat aatcatcgag cgccaacttg
    16561 cgaatatcga tgccgtcgaa caacaccgaa ccactcgcca cgtcgtaaaa tcggcaaatc
    16621 agattggcga cggtgctctt gcccgaacca gtgtggccca cgatgccgag catttggccc
    16681 ggcgcgacgc ggaaggtgag atcgtccaaa accaagcggc tcccgtagcg gaaagacacg
    16741 cgctcgaagc gcacctctcc ctgcggcgtg cgcagcgcgg caggctgctt gggctcgagt
    16801 acgctgggca cgcgatccaa gatctcgaac aggcgctgcg ccccagccgc tgcgcgttgc
    16861 gtagccgtca gcatgcggct catgctctcg actcgcacgt agaagcgccc aatgtaagcg
    16921 atgaaagcgg tgaggatccc gaccgttacg cgctggtgaa gcacctgata ggcacccacc
    16981 gcccagacca cgagtaggcc aatctggttg agcagcgcga ccaacggcca aaagaacgtc
    17041 cacaacgcgt tgacgcggct gttgacctcc acgatgcgct cattggcttg cgcgaagcgc
    17101 gctatttcgc ggcgctcttg agagaacgcc ttcaccacgc ggatgcccgg gatggtatcg
    17161 gccaagatgt tggtcatcgc cgaccacacc cgccctccgc gcagaaagcc ttgcgtcaat
    17221 cgcccacgaa cccggacgat tagccaagcg atcggtggga aagagacgat cgcagccaga
    17281 gccaaaatcg gatcgagcgt gagcagcacc acgcacgtgc ccacgatcat cagcacgtcc
    17341 gtgatgaagt cgatcagaga gtctgacagg aaggagcaca ggtgctcggt gtccgtgctg
    17401 atacgtgcga tcagatcgcc ggtacgtttg ccgccaaagt actccaggct aagccgttga
    17461 agatgtgcga aggcgcgatt cctgaggtcc gccgcgatct gctcggtggc ccgtgccaac
    17521 accgcgcctt gagcccagtg gagtccccag gcgaataggg cggcgagccc caagccgcca
    17581 aggtacagag ccacttgtcc gtacgcctgc tccgcttgca gacgtccggc ttgccccggt
    17641 acgagcacgc gatcgaccag cggcatggtg agatagggcg ggaccaaacc cacacccgtg
    17701 gaagtgatcg tcagtgccag ccccaaccct accgcgccga ggtgtggacg agcgaacgcc
    17761 aacagccgca gcaacggcat gcgcaacgag gcgagcgacg gctcctccgc ctccagcgtt
    17821 tcctcagcct cctcctcgtc tatgctcggt cgtgacgaat gactcttgtg gtggtgggcg
    17881 accgcagttt cgaaagcatc cacgaaagca tgcgcggcgc gcgcccgctc caaagtaaac
    17941 cgcgcgcgca gcaagcgcgt ttcgccgtcc caaaccgaca actcggacag cccgccatgc
    18001 tctcgacgct ggatttccag gttggaccgc aacgggatgc ggctctccgc ttggccctct
    18061 tcccggtacc gcagctcggt tcgcgtcagc accaactccg cacgaccaaa ccgcagatcc
    18121 tgagtgagat ccgcgtcgaa gcgagccaat ggacctgagc ttggagcgga tactggagaa
    18181 gcgggctcgg tgcgagagag gtcgggaagg cgtgcggtca ttgtaaagct cgggcgagga
    18241 cggagcggag cggacgttga gccggccggc ggttaaacgc aagtccgtga atgggacacg
    18301 actggcgcgg ctggcgcgcg cgcagagctt ggatcaggct tgcgccccac gcgactgggc
    18361 cttgcgaaaa accgctgccc caggcacaag ccggctcacc gaaagtcggc ctgaaaagcg
    18421 catccgcaaa cgagagacgc ctcccacgga gcgcccacga acagagcccg gacgagctga
    18481 aaggcttcga tggaaatccg caagatcaac gttttggttg gcccgaatat ctgggcgaat
    18541 tttcccgtgc tcgaagcctg ggttgacctc ggtcacttcg aggacttccc gtccaacaag
    18601 ctgcccggct tcaacgaccg gatcatggcg tggctgccca gcatggtcga gcaccgctgt
    18661 agcgtgggcg aacgcggggg tttcttcaag cggatgcgag atggcacgtg gctcggccac
    18721 atgctcgagc acgtctcgct ggagttgcac tctttgagcc acagccccgt cggttatgga
    18781 cgcgcgcgtg agacgagcga gcgtggcgtc tacaaagtcg tgatcgaatg cgacgatccg
    18841 gagttcgggc gcgcgtgtct gttgaccgca cgggagctga ttttggccgc ggtcgacgat
    18901 cgtccgttcg acttgagcgg cgagctgaag aagctgcgcg aggcggccga tcagtattgc
    18961 tacggcccga gcaccgaggc gattgtggag gcggctcgcg cacgtggtat cccgacaatc
    19021 cggctcaccg agcacaatct ggtgcagctc ggttacggca agagccagcg acgaatttgg
    19081 accgcagaga cggactccac gggcgcgatc gcggaggcca tcgcgcagga caaagacctc
    19141 acccgtaagt tactgagcgg ggtcgggatc ccagtgcccg cggggcgcac ggtgaccagc
    19201 gccgaggatg cctgggccgc agcggaagat ttgggcttgc ccgtggtcgt gaagccacaa
    19261 gatgcaaacc atggccgcgg agtgtccatc cgcatcgagg accgcgaggg agttcagacc
    19321 gcgtaccaag tggccctcaa ggaaggcagt ggcgtggtcg tcgagcgttt cgtgcccggc
    19381 actcactacc gcgtgctggt ggtcggagag cgggcgatcg cggcgagtgg cggcaccgcc
    19441 aaccaactgc tcggcgacgg aatttccacg gttgagcgct tggtggagct cgccaaccag
    19501 aaccccgagc gcagcgacga ttcggcgcaa ccactggccc cgttcgtact cgatacgatc
    19561 gcgctagagc tattgcgacg tcaggggctg gagccgaaga cggttccgga agcaggtcgg
    19621 cgcgtgttga tccgttacaa cggtgatctc accgtcgacg aaaccgcgcg catgcacccc
    19681 gaagtggcgg cgagttgcgt gctcgcagca caaactatcg ggctcgacgt ggcagggatc
    19741 gatgtgattg cggaagacat cgggcgcccg ctcgagcgac aagggggggc gatcatcgag
    19801 gtcaactcga gccccggttt gatcatgcac ctcaagccac tgaccggaaa accacagccg
    19861 gttggtgaag caatcgtcaa tcaccttttc aaggcgacgg acgaagggcg tgtgccgctg
    19921 gttgccgtga cgggcaccaa tggcaaaacc ccggttgcgg gcgcgctcgc tcacatgctc
    19981 gcggccgcag ggcgatcggt cgggcgcgcg gactcgactg gcgtgcacgt ccgcggtcgt
    20041 cagctcgcga aagagaacgg ctgcaccgca cagagtgcgc ggcgcgtact gataaatcct
    20101 ttcgtcgacg cgatcgtgct cgaagtttcc gaagcggatg tcctcgatca ggggctcgcc
    20161 ttcgatcact gcgatgtcgc ggtgatcacc aacattgcgt gcggtgatca ccttggcaaa
    20221 cgctatgtcg acgagctcac gaccatagaa aaggccattc gcgcgccgct cgatacggtg
    20281 cgcgccaagg gtgtgggagt gctcaacgca tcggatccgg cggtcgtcgc catggcggat
    20341 aaatgccgag gcgaagtgat gtggttcagc cgatcacccg accaaatcga agctcacctg
    20401 agccacgggg gccgaggggt caccgtagag aatgaccaaa tcgtccttcg aactgcccag
    20461 cgacgtgaat acgtcctgcc aacctcccgt ctgtcctgcg ccgtgctggg tctcccagac
    20521 atgcttctcg ataatgcatt gacttcggtg gctgcaggtt gggctttggg cctgaaccta
    20581 actgcgctgc gtgccggctt ggagcagcag ctcagccctg ccgaaggcgt gctctatgag
    20641 cacaaaggtc gcttaattat cgttagccga gcccgaaatg ccgccgcctt gggcggctgg
    20701 ctgcaggtgc tccaaacccg attcgcggat cgtcgctggc gctgcgtcgt agagccgcct
    20761 cgagattggc gaattcaaga tgcccaggct ttgggcaaaa ccctgtcttc ggccttcgct
    20821 cgggttcaag cgatttacac gcatgaaaac caggctttag ccgaagcact cgagactgaa
    20881 acccaagatc cggctagccg accttcgggt cccgtgaccc gtttctgcga ggcagtgaat
    20941 cagatgcttg aagcatcgga agccaccgac tcgctgttcg ctcagccggc cagcgctcgc
    21001 gactacgacg ccttgatttc tcttttggag aacaagggag ctgtggcgag gagggtttcc
    21061 ggtctcgtct ccgtagagaa ctgccgatga aactcgactc catacgcgcg ctgcgcggcc
    21121 ccaacctttg gtctgacgaa accgtcctgg aagccagtct cctgggtgat ctttcgggct
    21181 tcggtgggga gacgttggcg cggcttcgcg cagccttgcc gcaggtcgtg gctgaccggc
    21241 tcgcggaaac cacggcgctc gaagccaggc tcgaggggga tatcgcggag cagacctccc
    21301 tgcattgggc acgtttggtg ggtgctctcg cctgctctct gcagtacgcg acgggtgcgg
    21361 acgttggtta tcatcatgcg caggaatggg gcagcccacc ctgttcgagc aaacaacccg
    21421 gttcgagcaa acaaccgcgc actgaacacc gaatcgtcgt tgaatacgcc gaggaggaga
    21481 tcggtcgcaa agcgctggcc atggccgtgg agatcgtgct cgcagcgcga gcggggacgt
    21541 ctctcgatct cgaagcgcaa ctcacggagc ttcgccacgc ttacgagaac gagcggctcg
    21601 ggccaagcac gggatctatc gtgcgggcag ctctcgatcg cggcatccct gcgcgacgtc
    21661 tgacgagcgg cagcctggtc atgctcggac atggcgcgcg gcagcgccga atttgggccg
    21721 ctgaaacgga ccgcacgagc gccattgccg aagcgatcgc gcaggataag gagctgacca
    21781 aatcgctgct gcgtgccgca ggtgttcctg tacccgaggg ccgtccggtg gagagccccg
    21841 aacaagcgtg ggaagcggct caggaagtgg gattgccggt cgtggtgaag ccccgcaacg
    21901 gcaaccaagg ccgcggcgtc tcggtgcgac tgagcagcga agaggccgtg aagctcggct
    21961 atgagctcgc gcgcaagcag agcagcgaga tcatcgtcga gaagcatgta gaaggcgccg
    22021 atttccggtt gctcgtgatc gggggcaagc tcgtcgcggc ggcacgtcgc gagccgccca
    22081 aggtgattgg cgacggccgc aacgatattg ctgagctcgt gcgcgtggag aacttggacc
    22141 cacgtcgtgg ggacgaccat tcgacctcgc tcagcaagct acggctggac gagatcggcc
    22201 tggagatgct ggccgaacaa ggtctagctc cagagagcgt gcccgcggcg ggccaagtgg
    22261 tggtgctgcg gcgcaacgca aacctgagca ccggaggcac cgccgccgat gtaacggatg
    22321 aggttcatcc ggaggtggcc aagcgtgtcg cggacgccgc cgccatgatc ggattggaca
    22381 ttgcgggcat cgacgtcatg gcgccgcgcc tggacattcc gctcgagaag agcggcggag
    22441 ccatcgtcga agtcaacgct gcaccgggat tgcgtatgca tctcgagcct tcgagcggca
    22501 agccccgcaa cgtaggctcg gccatcatcg agtcgatgtt cggtcgtgga gatgacgccc
    22561 ggattccgat cgtcgcgatc acgggcacca acggcaagac cacgaccacc cgttgcgtcg
    22621 cgcaccttct gaagcaacac gggctgcgcg ttgggatgac gtgcacggac ggcatctacg
    22681 tcgaggggcg tcgcatcgac accggggact gcagtgggcc aaagagcgcg cgcgtggtgc
    22741 tcggtcaccc tcgcgtggac gcggcggtgc tcgagacggc gcgcggcggg atgttgcgag
    22801 aaggcctcgg attcgactgg tgcgatgtcg cgatcgtcac caacgtgggt gaaggcgacc
    22861 acttggggct gaacgggatc tacacggtcg aagatttggc gcgcgtcaaa gaaatcccag
    22921 tgcgtcgcgt gtcgccgcgc ggtgcggccg tgctgaacgc ggatgatccg ctggtggtcg
    22981 cgatggcagg cgcatgc
//
LOCUS       DASTKU010000185         4830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_927161, whole genome shotgun sequence.
ACCESSION   DASTKU010000185 DASTKU010000000
VERSION     DASTKU010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927161"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VFQ61_07075"
     CDS             <1..418
                     /locus_tag="VFQ61_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9954247.1"
                     /translation="IAAGAAGLIRRALDESRAYALERKTFGVPIAQHQAVQFMLADMA
                     IAHEATRLLCHKAAQMVDSGQADSVVSSYAKAYSADAAMRATTDAVQVFGGYGYTKEY
                     PVEKLMRDAKLLQIYEGTSQIQRIVIARNLLSKQGQ"
     gene            complement(456..1358)
                     /locus_tag="VFQ61_07080"
     CDS             complement(456..1358)
                     /locus_tag="VFQ61_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954248.1"
                     /translation="MRSLHSFGACALALFFVSLPGSAWALDKQGKPHVFGAEEPDLNL
                     AGALSFGPALFNPAYAARPDNTGKALMRYALHLDLELLGPRLTLPVDINTFTDRDRSG
                     FKKLSPTELDLIAGVASTWPVGPGSLELSSHAEQDRALDRHVPIQTYVDVRSRYLCSA
                     SSIWTQRTRNPLRPDFSAAMTIGWFAYNPSYFARPDNTGRALLRYNPHLEFSLYDGAL
                     AVGLDATFFTDRKSSALLPSELDLTPELRGQFDRYELHLAYERDMPVDRAGLVQHFAY
                     ALAVVNFDFAEDLGGPRPPRERHN"
     gene            complement(1467..2822)
                     /locus_tag="VFQ61_07085"
     CDS             complement(1467..2822)
                     /locus_tag="VFQ61_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9954249.1"
                     /translation="MASTTPESQSQPALARTMGLWALVIYGVGDMLGSGIYALIGKAA
                     GLMGNAVWLAFVVSMIAALFTGLSYASLGSRYPRAAGAAYVAHRAFGFPLLTYVLGLS
                     VVASGLTSFATQSRAFAGYFLGLTGWGPSATVLVALGFIAVLTFVNFWGMRESTALNV
                     VCTTVEASGLLIVLLAGLRYWGSVDYLETPIQPNGMSGLSVGLVLQGGVLTFFSFVGF
                     EDMINVAEEVKNPRKNFPLAVVIALAFTTVIYIGISITAVSVVPYAELGHSTQPLVDV
                     VRKAAPGFPPRAFSFIALFAIVNTGLLNYIMGSRLVYGLARQGFVPSFLGRIHRQRRT
                     PHFAILTLMCIVTLLALSGGVAELASATSVLLLTAFIIVNGSLIALKRRKDEPPGAFE
                     IPSFIPALGIVACAAMLLNAKGKSVLMAGLLLAVILVLYLVLRPKVVGEPELESDPSL
                     D"
     gene            complement(2934..4049)
                     /locus_tag="VFQ61_07090"
     CDS             complement(2934..4049)
                     /locus_tag="VFQ61_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954250.1"
                     /translation="MASNGCSGIGVRRKSGVALVASVVSVIAACSNNSSPIPEGASAA
                     SSTSGSGTTASSGGAQNGGAGISTSNSSGPRGGSTTSGGAITSGGATTSGGAMGGNTS
                     VATLDRFGVRMIYPTLMGGMEWYAKWTSGARTFEGQDPEDPWFDADHGDATYRVDGDG
                     TLKISGAIPRMYIHDPDLEKQWRNVEMTMYFRRVDDSGTAYAGLTGIARSNHGTIGRE
                     TQNLCDTRGLGARMRYDGAFDFEKETSHPESTAVARILHWPGGMPYDKWFGYKYVVYD
                     LADGNVKQELYLDETDGRDGGQWLLVNEHVDDGSNFGVQGVPCASGIDPAIKLTASAD
                     RPGSETGKPNITCYFRSDGVGTDGLWYKKGSVREISR"
     gene            complement(4137..>4830)
                     /locus_tag="VFQ61_07095"
     CDS             complement(4137..>4830)
                     /locus_tag="VFQ61_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HET9954251.1"
                     /translation="LDVAGGGSRDGANVQQYTCNGTSAQRWAVQAMGNGQYRLMPQTA
                     NNRCLDIDNAAPANGTNIQQWTCNSSVAQAFQLAFDTTGPQPIPNGTYRISPGTGAGR
                     CLDVAGGSTADAGNLQQWECNGTDAQRFRFRSLGGTSYEVRASVSEMCVEVAYSGTWN
                     GANIHQWPCGGTAAQTWSVETLGAGRYRFAPQVASGKCMDVSGGSASNGANVQQWSCN
                     ATGAQSFAVLAP"
BASE COUNT          986 a         1472 c         1497 g          875 t
ORIGIN      
        1 gattgcggcg ggcgcggccg ggctgattcg gcgagcgctc gacgaatccc gggcctacgc
       61 gctcgaacgc aagacgttcg gggtgcccat tgcgcagcac caagccgtcc aattcatgct
      121 ggcggatatg gcgattgcgc acgaggcgac gcggctcttg tgccataagg ctgcgcagat
      181 ggtggactcg ggacaggccg atagcgtggt ctcgagctac gccaaagcat actcggcgga
      241 cgcggctatg cgcgcgacca cggacgcggt gcaggtcttc ggcggctacg gctacacgaa
      301 ggaatacccg gtagagaagc tgatgcgaga cgcgaagctc ttgcagattt acgagggaac
      361 gagccaaatt cagcgcatcg taatcgctcg taacttgctt tcgaagcaag ggcagtaggc
      421 acttcccatc tcagcgtaga ccgcggccgt actggctagt tatgccgttc tcgcggcggc
      481 cgcggtccgc ctagatcttc ggcaaagtcg aagttgacga cggccagagc atacgcgaaa
      541 tgttgcacca gccccgcgcg gtcgactggc atgtcgcgtt cgtaagcgag atgcaattca
      601 tagcgatcga attggcctcg cagctcgggg gtcaggtcga gctccgatgg caatagcgcc
      661 gagctcttgc gatcggtaaa gaaggtggcg tcgaggccca ctgccagagc cccgtcataa
      721 agggagaact cgaggtgcgg attgtatcgg agcagcgcac gcccggtatt gtcgggtcgc
      781 gcaaaatagc tgggattgta ggcgaaccaa ccgatggtca tcgcggcgct gaaatcgggc
      841 cgcaaggggt ttctcgtccg ctgcgtccag atgctggagg cggagcacaa ataacgagat
      901 ctcacgtcca catacgtttg gatcgggacg tgacgatcga gcgcgcgatc ctgctccgca
      961 tgagaactca actcgagact accggggccg accggccaag tgctcgcgac gccggcaatc
     1021 aaatccaact cggtaggtga aagctttttg aacccgcttc tatcgcgatc tgtaaaagta
     1081 ttgatgtcca ccggtaacgt tagacgcggc ccgagaagct ccaaatctaa gtgtaaggcg
     1141 tagcgcatga gcgctttacc ggtgttgtcg gggcgtgcgg cgtaggcagg gttgaacagc
     1201 gcgggaccga agctgagcgc acccgccaaa ttgagatcgg gttcttccgc cccgaaaacg
     1261 tgaggttttc cctgcttatc gagggcccat gccgagccgg gcaaactcac gaagaacagc
     1321 gcgagagcgc atgcaccgaa cgaatgaaga gagcgcaagc agctgagtga agcagatccc
     1381 gtactctgcg ccaccgctga acgcacctcg tgcggtctat tggcgtgttc atctcgagca
     1441 cgtcgactcg agaaatccat ggcccatcag tcgagactcg ggtcggactc caactccggc
     1501 tctccgacca ccttgggtcg taagacgaga tagaggacga ggatgacggc gaggagtagc
     1561 cctgccatta gcacggactt gcccttggca ttcaacagca tcgccgcgca ggccacgatg
     1621 ccgagggccg ggatgaacga ggggatctcg aacgcgcccg gtggctcgtc tttgcgtcgc
     1681 ttcagcgcaa ttaaggaacc gttcacgatg atgaaggcag tcaagagaag tacgctggtt
     1741 gcgctggcca gctccgcaac cccgcccgac agcgcgagca gcgtcacgat gcacatcagc
     1801 gtgagaattg cgaagtgggg agtacggcgc tgacggtgaa tcctcccgag gaagctcggg
     1861 acgaagcctt gtcgcgccag cccatagacc aagcgagagc ccatgatgta attgagcagc
     1921 ccggtattga cgatcgcgaa tagagcaatg aaagaaaatg ctcgcggtgg aaagccaggc
     1981 gcggccttgc gcactacgtc gaccaacggc tgagtcgagt ggccgagctc ggcgtaagga
     2041 actaccgata ccgcggtaat gctgatcccg atatagatca cggtcgtgaa cgcgagcgcg
     2101 atcacgactg cgagggggaa gtttttgcgc ggattcttca cttcttcggc cacattgatc
     2161 atgtcttcga agccaacgaa ggagaagaac gtcaatacgc cgccctgcaa aacgagcccg
     2221 acggacaagc cactcatgcc attcggttga atgggtgttt cgaggtaatc aaccgagccc
     2281 cagtatcgca aacctgcgag cagtacgatg agaagcccgg aagcctcgac ggtcgtgcac
     2341 accacgttga gtgcggtcga ctcgcgcatg ccccaaaagt tgacgaacgt gagtacggcg
     2401 atgaagccga gcgcgacgag cacggtggcg ctcggacccc agcccgtgag ccccaggaaa
     2461 tatccggcga acgcccggga ttgggtggca aacgaagtta gccccgaggc gacgacggag
     2521 aggccgagca cgtacgtgag caggggaaaa ccgaacgctc gatgagcgac gtaggccgcc
     2581 ccggccgcac gcggataccg cgacccgaga gaggcgtagc tcaagcccgt aaacagcgcc
     2641 gcgatcatgc tcaccacgaa agccaaccac acggcattgc ccatcagacc ggcggctttg
     2701 ccgatcagcg cgtatatgcc ggacccaagc atatccccga cgccgtagat cacgagcgcc
     2761 caaaggccca tggtgcgagc caacgcgggt tgggattggg attcaggtgt agtggacgcc
     2821 atatcgcgct ccagagtaca ccgcttcaat ccggatcgct catggttcgg cgcgcgcccg
     2881 cgcgggagtc gtttcgacag cgttgagtag ccgctttacg acccgacgtg ctgtcaacgc
     2941 gagatctcgc ggacgctgcc tttcttgtac cagagcccgt ccgtgccgac gccgtcactt
     3001 cggaagtagc atgtaatgtt gggcttaccg gtctccgatc cgggacgatc agctgaggcg
     3061 gtgagtttga tagcgggatc gatgcccgag gcgcagggga cgccttggac tccgaaattg
     3121 ctgccatcat caacgtgctc gttcactaat agccactggc cgccgtcacg tccgtccgtc
     3181 tcgtccagat agagctcctg ctttacgttg ccgtcggcca ggtcatagac gacgtacttg
     3241 tatccgaacc acttgtcata aggcatcccg ccgggccaat gcaggatgcg agccaccgca
     3301 gtggactccg ggtggctcgt ttccttctcg aaatcgaacg cgccgtcgta gcgcatgcga
     3361 gcgcccaggc cgcgagtatc acagagattc tgtgtttcgc gaccgattgt tccgtgattg
     3421 gagcgggcaa tccccgtcaa gccggcgtag gccgtcccgc tatcgtccac gcggcggaag
     3481 tacatcgtca tctcgacatt gcgccattgt ttctccaggt ccggatcatg gatgtacatg
     3541 cgggggatgg ctccggaaat cttgagtgtt ccgtcgccgt ctacgcgata ggttgcgtct
     3601 ccgtgatccg cgtcgaacca ggggtcttcg ggatcttggc cctcgaaggt tcgagctcca
     3661 gaggtccact tcgcatacca ctccattccg cccatcaggg tcggatagat catgcgcacc
     3721 ccgaatcgat cgagcgtcgc gacactggtg ttcccgccca tggcgccgcc gctggttgtt
     3781 gccccgccgc tggtgattgc gccaccgctg gtggttgagc cgccgcgtgg gccgctcgag
     3841 ttgctcgtag atatgccggc cccgccattc tgcgctcctc cgctcgaggc ggtggtcccc
     3901 gagcccgagg ttgaactggc tgcggacgca ccctccggaa tgggagaact gttgttcgag
     3961 caggctgcga tcacggaaac cactgacgcg acgagcgcaa ccccgctctt gcgccgcact
     4021 ccgatcccag agcacccgtt ggaagccatg cgacgggaat gtaccccgag acgaagctca
     4081 cggctcagct tcgcgagccg aagagaatgc cggggccacg atcgaccgtc gctatcctat
     4141 ggggccagta ccgcgaagct ctgcgccccg gttgcgttgc aagaccactg ctgcacgttc
     4201 gctccattcg aggcgctccc gccgctcacg tccatgcatt tgccggaagc gacctgcgga
     4261 gcgaagcgat agcgccccgc gcccagtgtt tcgacggacc aggtctgagc tgcggtaccg
     4321 ccacatggcc actgatgaat gttagcgccg ttccaagtgc ccgagtaagc aacttcaacg
     4381 cacatctcgc tgaccgaggc gcgcacttca taagatgttc cccctaggct gcgaaatcgg
     4441 aagcgctgtg cgtcggtccc gttgcactcc cattgctgca ggttgcccgc gtctgccgtc
     4501 gagccgcccg cgacgtccaa gcagcgcccc gcacccgtcc ccggagagat acggtaagtg
     4561 ccgttcggga tcggctgagg ccccgtggtg tcgaacgcca gctggaaagc ttgggccacc
     4621 gagctgttgc acgtccattg ttggatattc gtgccgttcg cgggcgccgc gttatcgatg
     4681 tccaggcagc gattgttcgc cgtttggggc atcagccggt actggccgtt gcccatcgct
     4741 tgaaccgccc agcgttgcgc gctggtcccg ttgcaagtgt actgctgcac gttcgcgccg
     4801 tcgcgcgaac caccaccggc aacatctaag
//
LOCUS       DASTKU010000186         4514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_704641, whole genome shotgun sequence.
ACCESSION   DASTKU010000186 DASTKU010000000
VERSION     DASTKU010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704641"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..2552)
                     /locus_tag="VFQ61_07100"
     CDS             complement(30..2552)
                     /locus_tag="VFQ61_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HET9954252.1"
                     /translation="MRLKRQASHIATSFALLLPLSGAGLFSCAGQDEGSGADREPQHL
                     QATKQRLGDTEAEAVTKTMAPIRSLQTPGNVYMTSIEQTVATTGTFQYIVYAYQPDAT
                     GEGIRFELTARTSGGAVDSTHTFQSVILTSRPGEPGESQLALASGSGQRVYSFGTSRQ
                     GWYRLSFTHGSPSAGRTLGIKAYDASGNSLKMAWSDPPGVNAQVRVGLAVTQASNVFL
                     KGGAYRNTDPAQFAYADHVKVDGNLLYRRSFDQGRYNFPIGRLNAGQHTLEFSLNGSA
                     GDPKYWYGVNEKSFDTLGSKSAWDPVQFLYYGSMHTGDYDAWSEGVALAQGSTVPGSR
                     PPPVRRGTTFSVALTHPSLNTGNATIEIQSVFGRAKESWTRTLPADADYGGAIFVSGS
                     PSARHREHWRITVPADAPVGRYILRAFTPGSAQLGNPIQFYVIHNPYTLLASGAISKA
                     ELETYGYDEDEDGVAMQGPHGSDRDALRDHFTTYYDGSAELGYTPKTMITGAFRRTKD
                     ESSASMLDIAAASMDTTTNEFESMLRLYRIVAQRIRYQNPAFAHDSSETMVGGTLGPD
                     PSLAFWYSLPGTELEGLTGGQCYEYGTVLTALARSAGLLARPVSSAGAIAGWGNHIFA
                     EAYIPSLPQHGGKRTSSPTSANSDTDPWYAFDATDPNSVRLFPRAFTTYSEAVAPRAK
                     FGRAATLMQGPLSPGLDVVTNPTNWDPLSTAVVGPSGVASVTAAYASGPEFWLTASGV
                     TGWVGYGEKDFYRISKTITGARAVRVTALPGGSSSPSLNLKLCVGSVTSVPVMSDRCA
                     DAATSYPLPAGESYVVVFNDGEDMPLRFMRGDVTKYVLDLEY"
     gene            complement(2713..3933)
                     /locus_tag="VFQ61_07105"
     CDS             complement(2713..3933)
                     /locus_tag="VFQ61_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954253.1"
                     /translation="MKFAFVTGICVVLFQAAACSDAISQTSGAGATGSDADVSSAQSP
                     LISQNIAAMQIFAAGHGQLFDSKQQRIPADLQFVEQVQASVRTAALDSLGGQLSAELQ
                     SFMREADTLLSRQAVPSADKLVLKGGMAWRILQDAPGDVSAELEWRLEAVNAQIFSLT
                     PVKVNALYGDFLKRIGYWDYDARFGPLRLSYGKVCDLNQVPVPPDWAETGTAWVHVGR
                     LTHNILDTEGEADVYTYTDPVKRGACIALPRDGGLLGIICQSASTGKACFWDNIPRDG
                     SSRIPWKGATLRIGNLKNGADLTENCTNCHTGNNVFLTSPDDPVWAKLLRGPTTVDHP
                     RNFTLRVEASDDNRGGRPRYAPITGKPRPSWTNPYNSSTTCNTCHEAPKTELLGLMNP
                     PPMPPACGSACVTR"
BASE COUNT          837 a         1380 c         1412 g          885 t
ORIGIN      
        1 aggggaggcg gcgcaacgcc tcgaacccct cagtactcga ggtcaagcac gtatttcgtc
       61 acgtccccgc gcatgaaccg caacggcatg tcctcgccgt cattgaacac gaccacgtag
      121 gactcgccag cgggcagggg ataggacgtc gccgcatctg cgcagcgatc ggacatgacc
      181 ggcacgctgg tgaccgagcc gacgcagagc ttcaggttca aagaaggact gctgctaccg
      241 cctggtaacg ccgtgacgcg aacggcccgc gcgcccgtga tggtcttgct gatccggtag
      301 aagtctttct cgccgtaacc cacccaaccg gtgacgccgc tcgcggttag ccagaattcc
      361 ggacccgaag cgtacgcggc cgtgacagaa gcgacgccgc tggggccgac gacggccgtg
      421 gaaagcgggt cccaattcgt ggggtttgtc accacgtcga ggcctggaga aagcggccct
      481 tgcatcaagg ttgcggcgcg tccgaacttc gcccgcggcg ccaccgcctc agagtaggtc
      541 gtgaacgccc ttggaaagag ccggacagag tttggatccg tagcatcgaa agcgtaccac
      601 gggtccgtat cgctgttggc actcgtcggg ctgctcgtcc gctttcctcc gtgctgggga
      661 aggctgggaa tgtaggcctc tgcgaaaatg tggttgcccc agccagcaat cgcacccgca
      721 cttgaaacgg gcctggcgag caagcccgcg ctgcgagcca gcgctgtgag taccgtgccg
      781 tactcgtagc attggccgcc ggtcagtcct tcgagctcgg tacccggcaa gctgtaccag
      841 aacgcgaggg atggatcggg accgagcgtg cctccaacca tggtttcgga tgagtcgtgt
      901 gcaaacgcgg gattctggta tcgaatgcgc tgcgcgacaa tgcgatagag ccgtagcatc
      961 gactcgaatt cgttggtcgt cgtatccatg gaagcggcgg cgatatctaa catggaggcc
     1021 gaggattcat ccttcgttcg ccggaacgcg ccggtgatca tggtcttggg ggtgtaaccc
     1081 agctcggcgg acccgtcgta gtacgtggtg aagtggtccc gcagggcgtc gcgatcgctg
     1141 ccgtgagggc cttgcatggc aactccgtct tcgtcttcgt cgtagccgta cgtttcgagc
     1201 tctgccttcg agatcgcgcc gctcgcgagg agcgtgtacg ggttgtgaat gacataaaac
     1261 tggatcgggt tgccgagctg agcgctgccc ggcgtgaacg ctcgcaggat gtagcgaccg
     1321 accggcgcat ccgcgggtac ggtgatgcgc cagtgctctc ggtggcgcgc cgaaggtgag
     1381 cccgacacga agatcgcgcc gccgtaatcg gcatccgcgg gcaaggtcct tgtccaggat
     1441 tccttggctc tcccgaacac ggactggatc tcaatggtcg catttcccgt gttcaggctg
     1501 gggtgggtga gcgccaccga gaacgtagtt ccacggcgaa ccggtggtgg gcgtgagccg
     1561 ggcacggtgc tcccttgggc aagagcgaca ccttcgctcc aagcgtcgta gtcccccgta
     1621 tgcatggacc cgtaatagag gaactgcacc gggtcccacg cgctcttgct tccgagagta
     1681 tcgaagctct tctcgttcac accgtaccag tacttcgggt cgccggcgct gccgttcagc
     1741 gagaactcga gtgtgtgttg gcctgcattt agacgcccga tcgggaagtt atagcgcccc
     1801 tgatcgaagc tgcgacgata gagcaagttc ccgtcgacct tgacgtgatc ggcgtaggcg
     1861 aactgcgctg gatcggtgtt tctatacgct ccgcccttta ggaacacgtt cgaggcctgg
     1921 gtcacagcga gcccgacgcg cacctgagcg ttgacgcccg gaggatcgct ccacgccatc
     1981 ttcaaagaat tcccggaagc gtcgtaggcc ttgatgccca gagtgcggcc ggcgctgggc
     2041 gagccatgcg tgaacgagag gcgataccag ccctgccgcg acgtcccgaa agagtaaacg
     2101 cgctgtccgc taccactggc gagtgccagc tgagactccc ccggttcgcc gggacgcgag
     2161 gtgaggatca cggactgaaa cgtgtgggtc gagtccacgg cgcccccgct ggtccgcgcg
     2221 gtgagctcga atcgaattcc ttcacccgtc gcgtcgggct ggtacgcgta cacgatatac
     2281 tggaaggtgc ctgtcgtcgc gaccgtctgc tcgatagacg tcatgtagac gttccctggc
     2341 gtttgaagcg acctaatggg ggccatggtc ttcgtcacgg cttcggcttc cgtgtcgccg
     2401 aggcgttgtt tcgtggcctg caggtgctgc ggctctctat cggcgcccga gccctcatcc
     2461 tggcccgcgc acgaaaagag cccggctcca ctcaatggca gtagcaacgc gaaagaagta
     2521 gcgatgtgtg atgcttgacg tttcaatctc atgattcgac gacccatctt tttcaggcga
     2581 agttccggac caattaatga agggctatgt gcggcccgac tcttcccgag cgagtcgcgg
     2641 gaacgcgaag ctccgcagaa tcccgctgtg gccatccacc tcctggagcg ggagttggtt
     2701 gtcgcgccta gctcagcgcg tgacgcaagc gcttccacat gccggcggca tgggcggagg
     2761 gttcatcaag cccaagagtt ccgtctttgg cgcttcgtgg cacgtgttgc aggtcgtcga
     2821 gctgttgtat gggttcgtcc aactgggcct gggctttccg gtgatcgggg cgtagcgggg
     2881 gcggcctccg cgattgtcgt cagacgcctc gacacgcaag gtaaagtttc tcggatgatc
     2941 gacggtggtt gggccccgta gcaacttggc ccagaccgga tcgtccggcg aggtcaaaaa
     3001 tacattgttg ccagtgtggc agttcgtgca gttctcggtc agatccgcgc catttttcag
     3061 attgccaatt ctgagcgtgg cgcccttcca aggaatccgg ctactcccgt cgcgggggat
     3121 attgtcccag aagcacgcct tgccggtgct cgcactttgg cagatgatcc ccaataatcc
     3181 tccgtccctt gggagcgcaa tgcaggcacc gcgcttcacc ggatcggtat aggtatacac
     3241 gtccgcttca ccttccgtat cgaggatgtt gtgagtcagt ctgcccacgt ggacccaagc
     3301 cgtgccggtc tccgcccaat cgggcggcac agggacttga ttgagatcgc aaaccttgcc
     3361 gtagctcaac cgcagcgggc cgaaccgcgc gtcgtagtcc caataaccga tgcgcttcag
     3421 gaagtcacca tagagcgcgt tgaccttcac cggagtcagc gagaatatct gggcattcac
     3481 cgcctcgagc cgccactcga gctccgcgct cacgtcgccg ggcgcgtcct gcagaatgcg
     3541 ccacgccatt ccacccttca gcaccagctt gtccgcactc gggacagctt gacggctcag
     3601 caaggtgtcg gcctcgcgca tgaaactctg cagctcggcg ctgagctggc cgcccaagct
     3661 gtccaaggct gcagttctga cggaggcctg tacttgctcg acgaactgca aatcagccgg
     3721 gatgcgctgt tgtttggagt cgaacagctg accatggccc gcggcaaaga tttgcatggc
     3781 cgctatgttt tgggagatga gcggagactg cgcactggag acgtctgcat cgctgcctgt
     3841 tgcgccggca ccgctcgttt gtgagatcgc gtcgctgcac gccgccgcct gaaacaacac
     3901 tacacagatt ccggtcacga atgcgaactt catgtttgtg ttccccacgc ctttattgaa
     3961 tcgcgccacg acgctgctct cgcgatcgag tgcgtgaacg cagccctacg cagcactccg
     4021 attcctggac tagactcctg gactcgattc cacgctaagc aactgccgtg ccgcaccacc
     4081 cgtgaggttc gcggacgcag taagagcttc tccgtagacg atactgtccg gcgtggaggg
     4141 gccagagcgt tgggcttgca gtgctgcggg gtctcacggc aaggacgtgc cgacagctgg
     4201 cacatggcca aagttggtct ctactcgtgc caaagtcggc acgagtgcag ctcgatcgcg
     4261 gccgccaaca gttgcccgaa agaagattgc gattgactcg ccccgcgccg tcaccacaaa
     4321 ctgggctgat ggctacccta gcaacaagcg tagcgctact tacttggttg ggcgcagcgg
     4381 catgcgtggg aggtcaccac gtaaccaccg cgatgcaaag gtcggacgaa catctggctc
     4441 actgcggacg aggtacggat ggattgaccg actcaaccga cgagtgtcag ccggcgtttg
     4501 cagaatgctt cagg
//
LOCUS       DASTKU010000187         3232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_483065, whole genome shotgun sequence.
ACCESSION   DASTKU010000187 DASTKU010000000
VERSION     DASTKU010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483065"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            514..1020
                     /locus_tag="VFQ61_07110"
     CDS             514..1020
                     /locus_tag="VFQ61_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954254.1"
                     /translation="MGTIAAQLGVHHGTVRRVLAQAGLVTGPALGRPSIIDPFVPFIT
                     ETLTKYPRLRSSRLFAMVRERGYRGGEDYFRHVIARYRPKRTAEAYLRLRTLPGEQGQ
                     VDWAHFGKLTIGNAVRPLWAFVLVLSYSRKVFLRFYFGSAMPAFLHGHVSAFAYLAAS
                     RASFCTTT"
     gene            1017..1958
                     /locus_tag="VFQ61_07115"
     CDS             1017..1958
                     /locus_tag="VFQ61_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954255.1"
                     /translation="MKSAVLERSGDAIRFNPKLLELAAHYRFQAKPVAPARGNEKGRV
                     ERAIRFVRDSFFAARSFTDIDDLNQQARTWCDGEAADRRCPEDRARRVKEVFAEEQTR
                     LIALPPDGFPTAQIAQVEVGKTPYARFDLNDYSVPHEFTQRSLTIVASLEVVRILDGQ
                     SVIASHRRSWDRAQQVENPEHIQDLQRAKRKGRENRSLDRLYHLAPSSHELLQLVAER
                     GGNIGNHTVRLLQLLERFTARELNDAIDAALAQGTPHLGAIRQLLDKSRADRNELPPV
                     RLPLPQDPRLERLVVRPHSLSTYDSLRKDTTGDHNND"
     gene            1939..2706
                     /gene="istB"
                     /locus_tag="VFQ61_07120"
     CDS             1939..2706
                     /gene="istB"
                     /locus_tag="VFQ61_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HET9954256.1"
                     /translation="MTTTTTDPEALRDRVQRLGFFGLLASWNEIALEPWLPKLISLEE
                     QERKRRSLERRLRSARIGSFKSIADYDWTWPKRVDREAIDELLTLKFVEEGVNAVLLG
                     PNGVGKTMILRNLAHQATLRGHTVRFTTASDMLSDLAAQESSAALARRIRRYVQPRLL
                     CVDEVGYLSYDNRYADLLFEVVTRRYDGPRSIVLSTNKPFAEWSEVFPHAACTVTLID
                     RLLHRAELIDIEGDSYRLKEAKERAAKKSASRAKKRA"
     gene            complement(2750..>3232)
                     /locus_tag="VFQ61_07125"
     CDS             complement(2750..>3232)
                     /locus_tag="VFQ61_07125"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET9954257.1"
                     /translation="WGTLHSRIRKNRLYAQGRKATSTDAPYRWLNPVLFVMQLTLCTA
                     SFWTESPWLLAAHPSNAVRWVGVVLFCGGSGLYAWALAHLGANYSPCYDSCAPIRLVR
                     SGPYSRIRHPMYAGKIIVAIGTVVVSGSLWFVPTTIYLLAATLRAMRREDCCRLSQTA
                     "
BASE COUNT          629 a         1067 c          945 g          591 t
ORIGIN      
        1 cggatgcaga ggcaccgcga tagacgcaac tttccggcgc agatagtgac gcaacacgca
       61 tcgactttga ccgctcagag cgcgaaggat ggcgttcagg aggtcgccaa tgcgcgattg
      121 gaagacggct cttcaggtca gaccgacagg ggctctgctg cttgttacga gtccgggaca
      181 ggatgtgttg aaagcaatcc tgccgttggc gcccggccac ccacgagctc tgctcacgat
      241 tttggaggga ctggcgcttt ggcgcgggca ggcgttggag gttgtagttt ctgcggcggc
      301 gagctcagcg gattggcttg ggtcggggct gttcggcgac gagctgtttc ccggcgagag
      361 tcctctggtg cggtttcgag tcgctcaccg cgaccacccc ggccgccgcc tcaagggcgt
      421 cgctgacttt cgcaaccttc gccggagtgg gacgtgatct cgccagagcg cgaagcggaa
      481 attctgcggc tgcaccacgc tgagaagtgg ccagtgggca ccatcgcagc gcagctcggc
      541 gttcatcacg gtaccgttcg gcgcgttctg gcgcaggccg ggctcgtcac aggcccagcc
      601 ctgggccgac cgtccatcat cgacccgttc gtcccattca tcacagaaac actcacgaag
      661 tatccgcgat tgcgctcgag ccgtctgttc gcgatggtcc gcgagcgggg ctaccgcggc
      721 ggcgaggact acttccgtca tgtgatcgct cgctatcgcc ccaaacgcac cgccgaagct
      781 tatctgcgcc tgcggacgtt gccgggcgag caaggacagg tcgattgggc tcacttcggc
      841 aaactcacga tcggcaatgc cgtgcggcca ctctgggctt tcgtgctcgt gctctcttac
      901 tccagaaagg tctttctccg tttctacttc gggtcagcca tgccagcgtt tttgcatggt
      961 cacgtctctg catttgcgta cttggcggcg tcccgcgcga gcttctgtac gacaacttga
     1021 agagtgccgt gctcgagcgc tcgggcgacg cgattcgctt caatccgaag ctgctcgagc
     1081 ttgcggctca ttataggttc caggccaaac cagtcgctcc cgcgcgcggc aacgagaagg
     1141 gtcgagtcga gcgagccatc cgcttcgtgc gggactcgtt ctttgcggcc cgctcattca
     1201 cggatatcga tgacctgaat caacaggcca ggacttggtg cgacggcgaa gccgccgatc
     1261 gccgctgtcc ggaagaccgc gccaggcggg tcaaagaagt gttcgcagag gagcagactc
     1321 ggctgattgc gctgcctccg gatggctttc cgacagccca gatcgcccag gtcgaagtcg
     1381 gcaagacccc ctacgcgcga ttcgacctca acgactactc ggtgccgcac gaattcacgc
     1441 agcgcagctt gaccatcgtg gcgtcgttgg aggtcgtgcg cattctcgat gggcaaagtg
     1501 tcatagcgag ccatcggcgc tcctgggacc gcgcgcagca agtcgaaaat cccgagcata
     1561 tccaggatct gcagcgtgcg aagcgtaaag gccgagaaaa caggagcttg gaccgcctct
     1621 atcatctcgc gccgagtagt catgagcttt tgcaactcgt cgccgagcgc gggggcaaca
     1681 tcggtaacca caccgtgcgt cttctccaac tcctcgagcg attcaccgcg cgcgagctga
     1741 atgatgctat cgacgccgct ctcgcccagg gaactccgca cttgggtgcg attcgccagc
     1801 tcctcgacaa gtcacgagcc gatcgcaacg aattgccgcc cgtgcgcctc ccgctccctc
     1861 aagacccgcg gctcgagcga ttggtcgttc gcccgcactc cctatctact tacgacagcc
     1921 tgcgaaagga cacgaccggt gaccacaaca acgactgacc ccgaggcgct ccgcgaccgc
     1981 gttcagcgcc tcggcttctt tggcctgctc gcatcgtgga acgaaatcgc actcgagcct
     2041 tggcttccca aactcatctc cctcgaagag caagagcgca aacgccgcag cctcgagcgg
     2101 cggctacgct ccgcccgaat cggctccttc aagtccattg ccgactacga ctggacgtgg
     2161 cccaagcgcg tcgaccgcga ggccattgac gagctcctca ccttgaagtt cgtggaagag
     2221 ggcgtcaacg ctgttctgct cgggcccaac ggcgtcggca aaaccatgat tctccgcaac
     2281 ctcgcccatc aggccacgct ccgcggccac accgtccgct tcactacagc cagcgacatg
     2341 ctcagtgact tggctgcgca ggaatcgtca gcggcactcg cccgacgcat tcgtcgctac
     2401 gtccagccac gcctgctctg cgtcgacgag gtgggctatc tctcgtacga caaccgctac
     2461 gctgacctgc tgttcgaagt cgtcactcgc cgctacgacg gtccgcgctc gattgtactc
     2521 agcaccaaca agccctttgc cgagtggtcc gaagtcttcc cccacgccgc gtgcacggtc
     2581 accctgatcg atcgcctcct acacagggcc gagctcatcg acatcgaagg cgacagctac
     2641 cgcctcaaag aagccaaaga acgcgccgct aagaaatccg cctctcgagc caaaaagcgc
     2701 gcctagaaaa ccgaaacccg cggcgccgag ccaatcctcg gcgccagatt tacgcggttt
     2761 gcgaaagccg gcaacagtct tcgcggcgca tcgccctcag tgtggcggcc aacaaataga
     2821 ttgtggtggg cacgaaccag agcgagccac tcaccactac ggtgccgatc gcgacgatga
     2881 tcttgccagc gtacattggg tggcgaatcc ggctgtatgg tcccgaccga accaagcgaa
     2941 ttggtgcgca gctatcgtaa caaggcgagt agtttgcacc caagtgtgcg agtgcccacg
     3001 cgtagagtcc cgagccacca cagaacagca ccacgccgac ccagcgaacc gcgttactcg
     3061 gatgagcggc gagcaaccag ggcgattccg tccagaatga tgcggtgcag agcgtcaact
     3121 gcatcacgaa caacaccggg ttgagccacc ggtacggcgc gtcggtcgac gtcgctttac
     3181 gaccctgcgc gtagagacga ttcttccgga ttcgtgagtg caacgtcccc ca
//
LOCUS       DASTKU010000188         9038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_36896,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000188 DASTKU010000000
VERSION     DASTKU010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36896"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..2426)
                     /locus_tag="VFQ61_07130"
     CDS             complement(279..2426)
                     /locus_tag="VFQ61_07130"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9954258.1"
                     /translation="MDPLVRSALWLQRWVARALGTAISGALGAVLIWSARSEIGGLYK
                     ALFLLGLGLFAVYRLAQRLSRPPSEEDVVQLDLEVFSLLAVFAYGVVLRSPAGLDGPA
                     YAIVYALAMLAASYARPWAAALGVVFAVLLEAALTAWGSSGSGSGLERWLWHSVLMVG
                     FSLLNHLVFRAEIERVRKLSRVKVDSELRKMRESARTYRLLGAPTGAVDHSITPPPDD
                     PERLLRSGVDEIHQAVKFALWLLRKSLGLRTAMLLGLDSSGSQLNLQEIETSEDNVLP
                     GPFGARDGIFGAALEKLQQVSVSGPKASHHIGHYATRVAVGAASATPVLERGQTRGLL
                     VVDREAREPFSHEEEETLQHAARFIGRAIENERVFVQLERAKVEQGKLYRAANALASA
                     TTEAQVIEAGVSGAREFAAFDFAVVTLFDKTANEHEICAVSGDGASELVGQRFRHNSG
                     LVSMVVENRHALPYRGDYDSARQVVFTRRLNPPKMPSLLVLPLCVHDRALGTLVLGSK
                     RARAFGDSVRPTLEVLASHVAVSLANARMLTRLEELATLDGLTGLYNKRALTELAGQK
                     LRSATRFNKPLSLLVCDIDFFKKVNDTYGHDVGDLVIRGFGDVLRRVKRDTDAVGRFG
                     GEEFVLVCEETDERGAAQLAERIRSELEATTFHADSSPIHVTCSIGVAPFPAAGRTWE
                     TLFKATDEALYASKRGGRNRVTIWNPKLQGCAA"
     gene            complement(2491..3558)
                     /locus_tag="VFQ61_07135"
     CDS             complement(2491..3558)
                     /locus_tag="VFQ61_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954259.1"
                     /translation="MRSIVALVGLCFACGEAAHQSFEQTGGQAGRASGGQASGGTVSG
                     GNSSGGKNSGGGSVGGSTAGGAAGQSSGGQATGGRAFGGASGASGHSTSGGNAGAGGK
                     PSGGTGGVAQGGHAQGGKAQGGEGSGGITSFGGSAGSAGGGGVTGTAGSSGVGGAAGA
                     SSCSDPDSSGSTYPERVRSSALGTNGKFTDSCDASGNLVEYTCEYQTLDAQPCPLPAG
                     EAADPFVPPGSGGAAPPRPFCRVPTGRVVPMNVDCGGTCRDGACFSWCATFNDELHVV
                     SSMAGETAIENATRNVKYRCSVAFQPPGAAACEGAQVVGRTLFVTSIGNCTGTPLLTV
                     FGVSSSSTSAEECAYRCQPQL"
     gene            3746..5230
                     /locus_tag="VFQ61_07140"
     CDS             3746..5230
                     /locus_tag="VFQ61_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9954260.1"
                     /translation="MSSLRSSKAPFGSLARVRAGLRLQILVLLGGLMFVAFTPLYAAL
                     ATYTRVALEQLRASHARSLGRVIGAHVAEVGQNQSPQALETLLGSQVGSEGIEAIRVY
                     HPSGAVLGSVGAAEWFKVPMDVSRERALEVTSGHGRALAVVVPSTNVSVLAIVRLEPQ
                     RAEPLLRLFSLYTTLVALSLLLLTYFALTRFIVKPLSELSHAAQRVAQGARQLATPKT
                     SAHELHLLGHSLHAMTRRLLDEEEALRKKVDELEASQRRLTEAQQSLIASERLASVGR
                     LAAGLAHEVGNPLAALIGLQDLLLEGGLDASEQQDFLQRMRRETERIHRILRDLLDFA
                     RTGADLTREAGEPGDVYAAIHDTEALIFPQKQLQSIAWHTELERDLPRVKLSRERLVQ
                     VLLNLLLNAGDAVKPGGNVRLSARRQKDRVELCVEDDGPGVAPEVAARLFEPFVTTKE
                     PGKGTGLGLAVCRGLLEAVGGSIRYDIDHGPGARFVVSLPAVID"
     gene            5240..6781
                     /locus_tag="VFQ61_07145"
     CDS             5240..6781
                     /locus_tag="VFQ61_07145"
                     /inference="COORDINATES: protein motif:HMM:NF018435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9954261.1"
                     /translation="MRLGIDFGTTRTVVAAVRDGRYPVAAFEMGRGFVDFVPGLAVGS
                     NSEMVVGWDAVERLGGDAPSALRSIKRVVSSLSPDDTVAALAGQPTALDLTTEYLVRL
                     RRMIVERSNLDVPPDEPLEAMVAVPAGASTRQRYLTMEAFNRAGFRVLGMVNEPTAAA
                     IEYAHRTLGALSRRSPKRYVVIYDLGGGTFDTSAVSLAGRRYDLMATEGISELGGDDF
                     DEAIFALVMESLGKDPEELGSVARARALEACREAKETLNPSSRRMLIDLSGVAEGLPA
                     VTLETQQLYQRVEPLVRRTLALLDRVFSQLVEHGIDPNNPRELGAVYLVGGATAFPLV
                     GKLLRENYGRKVQLATEPHAATAVGLAIAADPEAEIYVREAVTRHFGVWREGDGGRDK
                     VFDPIFTKGSVSETGEHIAERRYSPVHGVGHLRYLECSEIGQFGQPGGDLMPWCDLFI
                     PYVPELADRQDLPSLATTRYLPEGAEQVVERYAYQRDGRVRVAIENETRGFRREFVLG
                     SPGAR"
     gene            complement(6853..>9038)
                     /locus_tag="VFQ61_07150"
     CDS             complement(6853..>9038)
                     /locus_tag="VFQ61_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9954262.1"
                     /translation="VEVIYDRADFVGRTLSTVMKNLLEGVAVVSVVLVLFLGSWRAAL
                     AVVLGVPAAMSIALFGMHLFDVTGDLMSLGAIDFGFLVDGPIVIVEAVIAATAGRPLV
                     GAARARAYDRIARGVARPVAFAVAIIMLVYVPLLSLEGVEGKMFRPMAVTMACALFGA
                     FVYSVVFFPAVLVALVTPAKGHGPHWISWISERYARLVPATLRFRWPLFAGALVSLVI
                     VGYAFGQSGAEFVPRIFEGDAVVTIRRSPSISLDEARKLDFETERVLQSYPEVITTIA
                     QTGRAEVAVDPVGNDNTDILTRLRPLPEWKRASDFDELSEQFKIAIENRVPGTFVSVS
                     QPIEDRTNEIISGSRADVQIQLFGTSLEVLSEVANKVGRSVRTVAGTGDVRVERVLGG
                     PTISAVPDRDLLARYGVSLQDAFDVVQAAREGVRVGNVFEQERKFDLRVLMPPVRPTA
                     ASLGDLFVRTSQGESIPLSEVIALSESEGPTAIRREDRRRAVRVDVNLRGRDLVSWVS
                     EARQKVEKEVPLPSGYNLTWGGQFENFERAQARLMIVLPVVVTIIFGMLLWMFQNWRF
                     ALAVFALVPLSLTGGMIGLLLSGLSFSLPAAVGFIALGGIGVLNGVVVGSGVRHLLDE
                     GVPLEEAICQGTSHVARAVLTTATVAALGFLPMAIATSAGAEVQRPLARVVVVGMLFG
                     TLLTLFVLPGVLRMVLQGYKASEDEDEVANEGLSEPQNGPVPAAV"
BASE COUNT         1677 a         2981 c         2878 g         1502 t
ORIGIN      
        1 cgcgcgcagc gcaatgcggg ttaggagggg ggacccgccg ggattcaccc cggcgggggt
       61 gagagtctgc tccagcgcgt gaagcgccga gactcacacc taagagttat ctcgtcgtcg
      121 caagcggggc tggggccccg cgcgcagcgc tttgcgagtt gggagggggg acccgccgga
      181 attcacttcc ggcgggggga gggtctgctt cagaccctcc caaaaaacac agccacttcc
      241 ggcgggggga gggtctgctt cagaccctcc caaaaaaact acgcagcgca cccctgcaat
      301 ttcggattcc agatcgtcac tcgattacgt cccccacgct tgctcgcgta gagcgcttcg
      361 tcggtggcct tgaagagagt ctcccaggtg cggccagcgg cggggaaggg ggcgacccca
      421 atggagcagg tgacgtggat tgggctcgag tcagcgtgga aggtggtggc ttcgagctcg
      481 ctgcggatgc gctcggcgag ctgggccgca ccgcgctcgt cggtttcttc gcagacgagc
      541 acgaactctt ccccgccaaa acgcccaacg gcgtcggtgt cacgcttgac gcgccggagt
      601 acgtcgccaa agccgcggat cacgaggtcg cccacgtcgt gcccataggt gtcgttgacc
      661 ttcttgaaga agtcgatatc gcagaccaac agactcaatg gtttgttgaa acgggtggcg
      721 ctacgcagct tttgaccagc gagctcggtc agagcccgtt tgttgtagag cccggtcaag
      781 ccgtcgaggg tagctagctc ttcgaggcgc gtgagcatgc gcgcgttggc gagcgatacg
      841 gcgacatggc tcgcgagtac ctcgagcgta ggccgcaccg aatcaccgaa ggcgcgcgcc
      901 cgcttcgagc ccaacaccag tgttcccaaa gcgcggtcgt gaacgcacag cggtagaacc
      961 aagagtgatg gcattttcgg cgggttgagg cgccgagtga agacgacttg gcgagctgaa
     1021 tcgtagtcac cgcgataggg cagagcgtgc cggttctcca cgaccatgga taccaacccg
     1081 gagttgtgcc ggaagcgttg gccgacgagc tcggaggcgc catcaccact caccgcgcag
     1141 atctcgtgct cgttggcggt cttgtcgaac agcgtcacga ccgcaaaatc gaaggcggca
     1201 aattcacgtg ctccgctgac acctgcctcg atgacctgcg cctcggtggt ggcgctggcc
     1261 aacgcgttgg ccgcacgata cagcttgcct tgctcgacct tggctcgctc gagttgcacg
     1321 aatacgcgct cgttttcgat cgcgcgcccg atgaagcgcg ccgcgtgttg aagcgtctct
     1381 tcttcttcgt gagagaaggg ctcacgcgct tcgcgatcga ccaccagtag cccgcgcgtc
     1441 tggccgcgct cgagtacggg cgtggccgag gccgcgccga ccgccactcg agtcgcatag
     1501 tggccaatgt gatgcgaggc tttggggccc gacacggaca cttgctgaag cttctcgagc
     1561 gccgctccga agatgccgtc gcgtgcaccg aatggccccg gcagcacgtt gtcttcgctg
     1621 gtctcgattt cttgcaggtt caactgcgag ccgctgctat cgagcccgag caacatcgcg
     1681 gtgcgtaggc ccaggctctt gcgcaggagc caaagcgcga atttgacggc ttgatggatc
     1741 tcatccacgc ccgagcgcag caaccgctcc ggatcgtccg gcggcggggt gatcgagtgg
     1801 tcgacggcgc cagtgggtgc accgagcaga cgataggtgc gcgccgactc acgcatcttc
     1861 cgcaactcgc tgtcgacttt gacccgtgac aacttgcgga cgcgctcgat ctcggcgcga
     1921 aataccagat gattcagcag cgaaaagccg accatcagca cgctgtgcca aagccagcgc
     1981 tcgagccctg aacccgatcc agagctgccc caggccgtga gcgccgcctc gagcagaacc
     2041 gcaaaaacaa cgccgagtgc cgcggcccac ggccgcgcat agctcgccgc caacatcgct
     2101 agcgcgtaaa cgatggcgta ggccgggccg tcgagacctg caggggagcg caggacgacg
     2161 ccgtaagcga acaccgccag cagcgagaac acctctagat ccagctgaac gacgtcctct
     2221 tccgaaggcg gacgcgagag ccgctgcgcc aaacgataga ccgcgaacaa tccgagcccg
     2281 agcagaaaca atgctttgta cagtccaccg atctcgctgc gcgcggacca aatcaggact
     2341 gccccgagcg cccccgagat agccgtaccc aaggctcgtg cgacccagcg ctgtagccag
     2401 agggcggacc ggacgagagg atccatggca tctccggccg tatcagctcg gccggagccc
     2461 ggctaagttt ttggagtcga tccgagcgct ctagagctgc ggctggcacc tgtaggcgca
     2521 ttcttcagcc gaggtgctcg aactcgagac accgaatacg gtgaggagcg gggtaccagt
     2581 acaattcccg atcgaggtga cgaacaaggt tcgtccgacg acctgagcac cttcgcacgc
     2641 cgccgctcca gggggctgga atgccacgct gcatcggtac ttgacgttgc gtgtcgcgtt
     2701 ctcgatcgcc gtctcgcccg ccatcgacga gacgacgtgc agttcgtcat tgaaggtagc
     2761 gcaccaactg aaacacgctc cgtctcggca agtgccgccg caatccacgt tcatcgggac
     2821 gactctgccg gtgggtacac gacagaatgg ccgcggtggc gcggcgcctc ccgagcccgg
     2881 cggaacgaag ggatcggcgg cttcgcctgc gggcaagggg cagggctgtg cgtccagcgt
     2941 ttgatactcg caggtgtatt cgaccaagtt cccgctcgcg tcacagctgt cggtgaactt
     3001 gccgttggtg cctagagcac tggaacgcac gcgctccggg tatgtgctgc ccgatgagtc
     3061 tgggtcgctg caactcgacg cgccggctgc gccacccacc ccactgctgc cggcagtgcc
     3121 ggtgaccccg ccgccaccgg cactgccagc cgaacccccg aagctcgtaa ttcccccact
     3181 gccctcgccg ccttgagcct tgccaccttg cgcatgcccg ccttgcgcga ccccacctgt
     3241 gccaccactc ggtttgccac ccgccccggc gttacccccg ctcgtactgt gccccgaagc
     3301 tccgctcgca cctccgaaag cgcggccacc cgtggcctgc ccaccgctac tttgcccggc
     3361 ggcgccgccc gccgtgctcc cacctacgga gccacccccg ctattcttcc cgccggaact
     3421 gttcccgccg ctcacagtgc cgcccgaggc ttgcccccca cttgcccgcc ctgcttggcc
     3481 tccggtctgc tcgaacgatt gatgcgcggc ttcgccgcat gcaaaacaca acccgaccag
     3541 agccacgata gagcgcatgc ggggagcggc ttcttcaatt cacatgccac ccacaacgca
     3601 ttgaattcat tgagccgaac acgcgacgcg gttcgttcag tggcacaacg tgagccaacg
     3661 acacagccct cccccagcgc atccgtcagc gccccgctaa tgcagcgctg cggaggagag
     3721 accgccttgg aagcatacgc ctcgagtgag ctcactgcga tcctcgaagg ctccttttgg
     3781 tagtctcgct cgcgtgagag ccgggctgag gcttcaaatc ttggtgttgc tcggcggctt
     3841 gatgttcgtc gcgttcactc cgctatacgc ggcgctcgcg acttacaccc gggtggctct
     3901 cgagcagctc cgagccagcc acgctcgttc gctggggcgc gtgatcggtg cgcacgttgc
     3961 ggaggtgggg cagaaccaga gcccacaagc actagagacc ctgctcggat cgcaagtggg
     4021 ctcggaggga atcgaggcga ttcgcgtcta tcatccgagc ggggccgtgc tcggcagcgt
     4081 cggcgccgcg gaatggttca aagtacccat ggacgtgagc cgagaacgag cgctcgaggt
     4141 gacgagtggc cacggacggg cgttggccgt cgtggtgccc tccacgaacg tgtccgtact
     4201 cgcgatcgta cgcctcgagc cacaacgcgc ggagcctctg ctgcggctct tttccctgta
     4261 cacgacgctg gtcgcgcttt ccttgttgtt gctgacctac ttcgcgttga cgcgcttcat
     4321 cgtcaagcct ctgagtgagt tgtctcacgc ggctcaacgt gtggcccagg gcgcgcgcca
     4381 gctggcgacc cccaaaactt ctgcacacga gttgcattta ctcgggcaca gcttgcacgc
     4441 catgacgcga cgcttgctcg acgaagaaga ggcgctccgc aagaaggtgg acgagctcga
     4501 agccagccaa cggcggctca ccgaggcgca acaaagcctg atcgcctcgg agcgcttggc
     4561 ctccgtcggg cgtttggcgg ccgggctggc ccacgaggtg ggcaacccgc tcgccgcgtt
     4621 gatcgggctc caggatctgc tgctcgaagg gggcctcgat gccagtgagc aacaggactt
     4681 cctgcagcga atgcgccgcg aaacggagcg gatacatcgc atcctgcgtg acttgttgga
     4741 ttttgcgcgc acgggcgccg atctgacgcg agaggccggc gaacccggag acgtgtacgc
     4801 cgcaatccac gacaccgagg cgctgatatt tccgcaaaaa caactgcaaa gcatcgcctg
     4861 gcacaccgag ctggagcgcg atttgccgcg cgtgaagctg agccgtgagc gactggtgca
     4921 ggttctgttg aatctgctgc tcaacgcggg agacgcggta aaaccgggtg gaaatgtgcg
     4981 gctcagcgcg cggcggcaga aagaccgcgt ggagctgtgt gtggaggacg atgggcccgg
     5041 cgtggcgccc gaggtcgcgg cgcggctatt cgagcctttc gtgaccacca aagagcccgg
     5101 gaagggcact gggctcgggc tcgcggtgtg ccgcggtttg ctggaagcgg tcgggggcag
     5161 catccggtac gacattgatc acgggcccgg cgcgcgcttc gtggtaagtt tgcccgcggt
     5221 aatcgactag cgtcacggaa tgcgactcgg aatcgacttc ggaaccacac gcacggtcgt
     5281 cgcggcagtt cgcgacgggc gctaccccgt agcggcgttc gaaatgggcc ggggattcgt
     5341 ggatttcgtg cctgggctcg cggtgggctc gaactcggag atggtggtcg gttgggacgc
     5401 ggtcgaacgg ttgggcgggg atgcgccgtc cgcgctccgc tcgatcaagc gcgtggtgag
     5461 ctcgctgtct ccggatgaca cggttgcggc gttggccggg caacctacgg cactggatct
     5521 gacgacggaa tatctcgtgc ggctgcgccg catgatcgtc gagcgaagca acctcgatgt
     5581 tcctccggac gaacccctcg aggcgatggt ggcggtgccg gcgggggcga gcacgcggca
     5641 gcggtacctc acgatggagg ccttcaatcg ggccggattc cgtgtgctcg gcatggtgaa
     5701 cgagcccacc gcggcggcca tcgagtatgc ccaccgcacg ctgggcgcgc tgtcccggcg
     5761 cagccccaag cgctatgtcg tgatttacga tctcggcggc ggcacgttcg atacctcggc
     5821 ggtctcgttg gcggggcgtc gctacgactt gatggccacc gagggcatct ccgagctcgg
     5881 tggggacgac ttcgacgaag cgatctttgc cttggtgatg gagagcttgg ggaaagaccc
     5941 cgaagagctc ggcagtgtgg cgcgcgcgcg ggccctcgaa gcttgccgag aggccaagga
     6001 gacgctgaac ccatcctcgc ggcggatgct gattgatttg agtggtgtcg cggagggctt
     6061 acccgcggta acgctcgaga cgcaacagct gtatcagcgc gtggagccgc tagtccggcg
     6121 cacgctcgcc ttgctggacc gcgtgttttc gcagctggtg gagcacggca tcgacccgaa
     6181 caatccgcgt gagctcggcg ccgtgtacct cgtcggcgga gcaacggctt tccccctcgt
     6241 gggcaagctg ctgcgtgaaa actacgggcg gaaggtgcaa ctcgcaacgg aaccgcacgc
     6301 cgcaaccgcc gtaggtttgg cgattgcggc ggatccggag gcggagattt acgtccgtga
     6361 agccgtgacc cgccacttcg gcgtgtggcg cgagggggac ggcgggcgag acaaggtgtt
     6421 cgatccgatc ttcaccaagg gctccgtttc agaaacgggt gagcacatcg cggagcggcg
     6481 ctattcaccg gtgcacggcg tgggtcacct gcgctacctc gaatgcagtg agattggcca
     6541 gttcgggcaa ccgggcgggg atctgatgcc ctggtgcgat ttgttcatcc cgtacgtgcc
     6601 ggagctcgca gatcgccaag atttgccgag tttggcgacg acgcgctatc tgccggaagg
     6661 ggcagagcaa gtcgtggaac gatacgctta ccaacgcgac ggccgggtgc gggtcgcgat
     6721 cgagaacgaa acgcgcggtt ttcgccgaga gttcgtgctc ggttcgcccg gcgcgcgtta
     6781 acaccggttc aggttaacac cggttcagcg acggccgagc ggcttcgaag ccgctcggca
     6841 tcacaggacc tcttacaccg ccgccggcac gggaccgttc tgcggctccg acaagccctc
     6901 gttcgcgacc tcgtcctcgt cttcggaggc cttgtaaccc tgcagcacca tgcgcagcac
     6961 gccgggcagc acgaacagcg tcagcaaggt accaaacaac atgccgacca ccaccacgcg
     7021 cgccagaggg cgttgaactt cggcgccggc actggtcgcg atagccatgg gcaggaagcc
     7081 cagcgccgcc acggtggccg tcgtcaacac ggcgcgcgca acatgcgagg tcccctggca
     7141 aatggcttct tcgaggggca ccccctcatc gagcaggtga cgcacgccgc taccgaccac
     7201 caccccgttc aacacgccaa tgcccccgag cgcgatgaag cccaccgccg cgggcaagct
     7261 gaacgacagc ccactcagca acagcccaat catgccgccc gtcaaagaca acggcacgag
     7321 cgcgaagacg gccagggcga agcgccaatt ctggaacatc cagagcaaca ttccgaagat
     7381 gatggtgacg accactggca aaacgatcat gagccgggcc tgcgcgcgct cgaagttctc
     7441 gaattgccca ccccaagtga ggttgtaccc ggacgggagc ggcacctctt tttcgacctt
     7501 ctggcgcgcc tccgagaccc acgacaccaa atcacgcccg cgcaaattga cgtcgacgcg
     7561 cacggcgcgc cgacgatctt cacgtcgaat cgccgtgggg ccttcgctct ccgataacgc
     7621 gatcacttca ctgagcggga tgctctcgcc ttggctcgtg cgcacgaaca gatccccgag
     7681 tgacgcggcc gtgggacgca cgggcggcat cagcacgcgc aaatcgaact ttcgttcttg
     7741 ctcgaagacg ttgcccacgc gcacgccctc gcgcgcggct tgtacgacgt cgaaagcatc
     7801 ttgcaaactc acgccgtagc gagccagcaa atcgcggtca ggaaccgcgg agatcgtggg
     7861 gccgccgagc acgcgctcca cgcgcacgtc ccccgtgccg gccactgtgc gcaccgatct
     7921 tccaaccttg ttggcgacct cggacagcac ctccaggctg gtgccaaaca gctggatttg
     7981 tacgtccgcg cggctgccgc tgatgatctc gttcgtacgg tcttcgatcg gctgcgagac
     8041 actcacgaac gtgccgggca cgcggttctc gatcgcgatc ttgaattgct cgctgagctc
     8101 gtcgaaatcg gaggcgcgct tccattcggg cagcgggcgc agtcgcgtga gaatgtccgt
     8161 attgtcgttt cctacgggat caacagccac ctcggcgcgg ccggtctggg cgatcgtggt
     8221 gatcacctcg ggataactct gcagcacgcg ctcggtctcg aaatcgagct tgcgcgcttc
     8281 gtccaacgag atgctgggcg atcggcggat cgtaacgacc gcatcgccct cgaaaattcg
     8341 aggcacgaac tccgcgccgg attggccgaa cgcgtagccc acgatcacca gagacaccaa
     8401 tgcccctgcg aagagcggcc agcggaagcg aagcgtggcc ggcacgagcc gcgcatagcg
     8461 ctcgctgatc cagctgatcc agtgcggacc gtggcctttg gccggtgtga cgagcgccac
     8521 caacacggcc gggaagaaca ccaccgaata tacgaaggca ccaaagagcg cgcacgccat
     8581 ggtgacggcc atgggccgaa acatcttgcc ttctacgccc tccaggctga gcaacggcac
     8641 gtacaccagc atgatgatcg ccaccgcgaa cgcgaccgga cgcgctactc cgcgcgcgat
     8701 tcgatcgtaa gcccttgcgc gggccgcgcc aaccagcggg cgcccggccg tcgccgcgat
     8761 cacggcttcc acgatcacga tgggcccatc caccaagaac ccgaaatcga tcgccccgag
     8821 cgacatcagg tccccggtga cgtcgaacaa gtgcatcccg aatagcgcaa tgctcatcgc
     8881 cgcaggcaca ccgagcacca ccgccagcgc agcgcgccag ctacccagga acaacaccaa
     8941 cacgaccgag accaccgcca cgccctcgag caggttcttc atcacggtgc tgagcgtgcg
     9001 gcccacgaaa tccgcgcggt cgtagatcac ttccacgc
//
LOCUS       DASTKU010000189         9920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_36940,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000189 DASTKU010000000
VERSION     DASTKU010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9920
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36940"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VFQ61_07155"
     CDS             <1..1000
                     /locus_tag="VFQ61_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9954263.1"
                     /translation="RGDRIGFVGPNGSGKSTLLKLLGARGSADDRGDVRRGTNLKEGY
                     FDQHLGSLNPDRTAVDEIRSVRGDLNVDGARQYLARFRFYGDDPLRRVASFSGGERTR
                     LALAKLLLEPRNLLFLDEPTNHLDIPAAEILEEALVGFEGTVLLVSHDRRFLENVTTR
                     TVAFTAEGIDIYEGGFKDYSVILERNARAKDPNKGDGRSSQNRPPEGKSRDNRKPSAD
                     NGGSKDATTKQSASASSKPQPDTGRPELGADAHRARRAQVREVERKQKRVSELESAIS
                     KSEAEVQTLREELRAASGSDWEKLHKLAGRERELTLQLERLTEEWLRLSEELQNVL"
     gene            1010..1924
                     /locus_tag="VFQ61_07160"
     CDS             1010..1924
                     /locus_tag="VFQ61_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET9954264.1"
                     /translation="MSSAERSWRKAGGLVREALRARKPEALRARKPEALRARKPEALR
                     ARKPEALRGKLMVGVVSLILGVLVGLGGCHKNSAEAHDDHGSADPGSAAPKSATLPPL
                     VLKADTPNLLLTWIDEQGDFHVVDKTSDVPAEARKTVRVVVTDREAGTGEQVYVANLS
                     APATDGSFSVTSMSRAAWDELGATKRKARLEALAPPPPSALASAAGSAPSAKPAASSV
                     VVIIYGASWCKPCHDAESYLKRRGVNVVKKDIEESEVAANEMRAKLERAGKGGASIPV
                     IDIGGRLLVGFSPAAIEKALETARTKTL"
     gene            2085..3026
                     /locus_tag="VFQ61_07165"
     CDS             2085..3026
                     /locus_tag="VFQ61_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9954265.1"
                     /translation="MADSDESPDARYSIRVASRLTGLSADTLRMWERRYGFPKPSRNG
                     SQVRFYSDADIERLGLVFRALKAGFRAGEVIHRETPELRDLLASSTAGPAQVSVVRES
                     PTVDALIEALRADDPSAVQSGLRRAVATLGPRQFITDVASGLVERVGEEWAQGRLGVR
                     HEHLLSEALSTQLRLLLSAYDNAGAPVVLLTAVSREQHSLGLEMAALYLALEGACPRL
                     LGVDTPPDQIVEAASALGARVIGLSISTAADTEVVSEQVRWILTELPPGVELWLGGKT
                     AGRLGLSDGRLFYTPTWPDVDRELARVRGRNSAVSAA"
     gene            complement(3062..3919)
                     /gene="purU"
                     /locus_tag="VFQ61_07170"
     CDS             complement(3062..3919)
                     /gene="purU"
                     /locus_tag="VFQ61_07170"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HET9954266.1"
                     /translation="MAKDLATLLVSCPDRRGIVAALAQVLYGHGANILDSDQHTDLHQ
                     QQFFQRIRFDLSELLTDRGTLERAIAEVAQRFSMQYRLVYAERPKRVVVFVSKYDHCL
                     YDLLLRRRANEFSCEIPLILSNHPDLAPVARDFGVPFEVIEKNASNKREAEQLERQRI
                     EEIGADLIVLARYMQVLSEDFVRGYAGRIINIHHSFLPAFIGGRPYHQAHERGVKLIG
                     ATAHYATAVLDEGPIIEQDVVRASHRDSVDDLVRKGRDLEKVVLARAVRAHLNDRVLI
                     YGNKTVVFE"
     gene            complement(3929..7858)
                     /locus_tag="VFQ61_07175"
     CDS             complement(3929..7858)
                     /locus_tag="VFQ61_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9954267.1"
                     /translation="MQALDGHAEGTTLGRCEMAEASQAKNPEEEPAEASEERQSAAPV
                     QPATAARAERAAAPAAAPPAAVTAPPPIPAAAPAAQAVPPNKLPPVHKPPTANRAVKP
                     RVATPAEEARRTDPNAVNDVEILSAVPEKDEDSAPPSIPSAAEVKSAKASPAGAPVLP
                     GQVIASRYEVMSLLGEGGMGIVYRCRDQSNGHQVALKRVIPPPGNLANEYLMWFYKEA
                     RALAALDHPNIVKARDFGQLRDGSPYLVMELVQGISMHDLAHTRVQFPVLWAITDRIL
                     SALAHAHARGIIHGDLKPSNILVERRDDEPPDVHVLDFGLAWLRQDWHDERLDGSKAM
                     EFAPHAGAGTPGYMAPEQIQHEMHYVCGATDLYALGCILYRIIAGRAPFSGESRELLR
                     VHAFEPPPKFTPVFEVPAGVEAFVMRLLAKRPWDRWEFAAECRRDWARFRPEERRGVW
                     FFPTPPRAQSEHPKTRPTGPRAQNPDLLPAPERAPGLLSIRPSPLVGRRDVRERLRAV
                     CDEVIQGGGAPHRLVLLVGPAGCGKSRIAEWLCETVHEEGTMVPLRSRYRKIRSSLDG
                     MLGAAVNYYNFERVDRNTIERSLLDRWKVRRDDKNGRAWVAGTAEWFRPMGPMSDQPI
                     GPSGIRFTLDTLETRRMVIRYTLRRIANGRPLLFWLDDLHNAAPTTFEGLLRTLTDDP
                     DQRIVMVGTVRSEDVALGTQTAERLRELRDAMQGVVIQIDPLPNETTLELLRASLPLD
                     DAAAQEAARRSRGNPLFALQQLHAWALAGSLELKDNFYRVPPDVLAVRPETTAELWDT
                     RLTAVPEAHRPSAHAAATLGGDIRRNVLFALLTALGQPADAAILSLQNAEILIPRGPG
                     RLSWPHELLQEHLLLRLGERPDKERIYRAAAAALTQHPLANTRRIVRQRVVNLMQAGD
                     ADSAAYLLFDFLQNSWNGAREPLATLDDLELFKNRLSGRTLALKNRWQAECLRHVGRT
                     AEASTYAELARTRFEELGDQENIAHCVRLLGHIASEQGSNAEGLELVQRALRTFEALG
                     SVLGQAHCQAVAGEIQYLLGSYEEARATIEQGERNFAALDQPLGRGQCLLLLSWIDHS
                     EGATERSRRLAQEARAEFERAGYRLGTAQADASLSHVEHRLMNFTGAERGALEALTVF
                     ETLRTPRGQAACDRLLAMIAVDTDDVELAERHAERSHRVFTRASDPWGIVEAMLLLCQ
                     AARMRHDLRRAVRLLEDSQRVPIEEAEPRQHLLLTRAWLECERGELDRALESIESAAE
                     VFGPRTRAGDHTPHLLGRLSRYRFSAQARDRIEAWRALLNDRARRRE"
     gene            7974..9242
                     /gene="glyA"
                     /locus_tag="VFQ61_07180"
     CDS             7974..9242
                     /gene="glyA"
                     /locus_tag="VFQ61_07180"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET9954268.1"
                     /translation="MATERELRDPPLSVRDPEIANLIKQEELRETVKLRLIPSENYAS
                     RAVLEATGSVLTNKYSEGYAGKRYYEGQQLIDQVEELACSRLKALFGADHVNVQPYSG
                     SPANLAVYLAFCQPHDPIMGLALPAGGHLTHGWNVSITGKYFKSVGYGVNRTDHRIDF
                     DEVARLAREHKPKLLWCGTTAYPRIVDFAKFREIADSVGAKLIADISHISGLVVGGVH
                     PSPVPVADVVTSTTHKTLRGPRGGMILCKAEHAKDIDRAVFPGLQGGPHNHTTAGIAV
                     AAREAATPEFKQYAENIVRNSKTLAEALLGQGFSLVTGGTDNHLILIDLTNKNIPGKV
                     AARALDRAGIVCNYNSVPFDPRKPFDPSGIRIGTPSITSRGMGPAEMQRLAGWMQQVV
                     AAPDNEAQLDKIAAEIKEMCSGFPPPGIPV"
     gene            9268..>9920
                     /locus_tag="VFQ61_07185"
     CDS             9268..>9920
                     /locus_tag="VFQ61_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9954269.1"
                     /translation="MTETTCSKLLARAERLMPGGVNSPVRAFKAVGGGPPFVARARGS
                     RLWDADGKEYVDFVGSWGPAILGHADPHVVQAVQQAAERGLSFGAPTELEVRFAERIT
                     RLYPSIELLRCVSSGTEATMSALRVARGFTRRDLIVKFDGCYHGHADPLLVKAGSGAA
                     TFGNPDSAGVPASIVANTATLSFNDFEALESLFAARGAEIAAVIVEPVVGNMGCVPP"
BASE COUNT         1762 a         3225 c         3228 g         1705 t
ORIGIN      
        1 gcgaggagat cggattggct tcgtggggcc caatggttcc ggtaaatcga ccttgctcaa
       61 gctgctcggc gcccgcggca gcgccgacga tcggggtgac gttcggcgcg gcacgaacct
      121 caaggaaggg tatttcgacc aacacctggg atcgttgaac cccgatcgca ccgcggtcga
      181 cgaaatccgc agcgtgcgcg gtgacctcaa cgtcgacggg gcgcgccaat acttggcgcg
      241 atttcgcttc tacggggatg acccgctgcg gcgtgtcgcc agcttttccg gcggcgaacg
      301 cactcggctg gcgctcgcca agctgctgct cgagccgcgc aatctgctgt tcctggacga
      361 gcccaccaac cacctggata tccccgccgc ggagatcttg gaggaagcac tcgtaggctt
      421 cgagggcacc gtactcttgg tctcccacga ccgtcgtttt ctcgagaacg tgaccacccg
      481 cactgtcgcg ttcaccgccg aagggatcga tatctacgaa gggggcttca aggattactc
      541 ggtgatcttg gagcgcaacg cgcgcgccaa ggaccccaac aagggtgacg gcaggtccag
      601 ccaaaacaga ccgccagaag gcaaatcacg agacaatcgc aagcccagcg cagacaacgg
      661 cggaagcaaa gacgccacca ccaagcaatc agcaagcgcg tcgagcaaac cgcaacctga
      721 tacgggtcgg cccgaactgg gcgcggacgc ccatcgggca cgccgggcgc aggttcgcga
      781 agtagaacgc aagcagaagc gagtgagcga actcgagagc gcaatctcca agagcgaggc
      841 ggaggtccaa acgttgcgcg aagagctacg cgccgcgagt ggcagcgact gggagaagct
      901 ccacaagctc gcgggacgcg agcgcgagct gaccctgcag ctcgaacgcc tcaccgaaga
      961 gtggctgcgc ttgtccgaag agcttcagaa cgtgctatag gccagggcaa tgtcgtctgc
     1021 tgaacggagc tggcggaagg ctggtggtct cgtccgagaa gcgctccgcg ccagaaagcc
     1081 agaagcgctc cgcgccagaa agccagaagc gctccgcgcc agaaagccag aagcgctccg
     1141 cgccagaaag ccagaagcgc tccgcggcaa gctgatggtc ggcgtcgtct cgttgatcct
     1201 cggcgttttg gtcgggctcg gtgggtgcca caagaacagc gccgaagccc acgacgacca
     1261 cggctctgcc gatcccggct cagcggcgcc gaaatcggcg accctcccac ctctggtgct
     1321 caaggcggat acaccgaatt tgctgctgac gtggatcgac gaacaaggtg acttccacgt
     1381 cgtcgataag acttccgatg tgcccgccga ggcgcgtaag accgtgcgtg tggtggttac
     1441 ggatcgcgaa gcgggcaccg gggaacaggt ctacgtcgcc aacttgagcg cgcccgcaac
     1501 ggacggctcc ttcagcgtaa cctccatgag ccgcgcggca tgggacgagc tcggggccac
     1561 gaaacgcaaa gcgaggttgg aagcgttggc gccgcccccg ccctctgctc tagccagcgc
     1621 agccggcagt gccccgtctg caaagcccgc ggcgagctcg gtggtggtga tcatttacgg
     1681 agcgagctgg tgcaagccct gccacgacgc agaaagctat ctgaagcggc gcggcgtcaa
     1741 cgttgtcaag aaagacatcg aagagagtga agtcgcggcg aacgagatgc gcgccaagct
     1801 cgagcgagca ggaaagggcg gcgcatcgat cccagtgatc gacatcggcg ggcgtctgct
     1861 ggttggcttc tctccggctg ccatcgaaaa ggcgctcgag acggctcgca ccaagacgct
     1921 ttgaggcccg cccccgccct aactcgtggc ttaacctgcc gcgcacgccg cgcgatacgc
     1981 gggttgcccg aggttgtaag cgccagcgaa agcatgacga ctggccgttg cacaatcctg
     2041 tctaaccttg acggtgtgtt gtcctggact aatcctgaac gggtatggcc gattcagatg
     2101 agtcccccga cgcgcgctac tcaatacgcg ttgcgtcaag gctgaccgga ctctctgcgg
     2161 ataccttacg tatgtgggag cgccgctatg gctttcccaa accttcccgc aacggttcgc
     2221 aggttcgttt ctatagcgat gcggatatcg agcggctcgg cctggtcttt cgtgctttga
     2281 aggctggctt tcgcgctggc gaggtgattc accgcgagac acccgagctt cgggacctat
     2341 tggcgagctc gacggcgggc ccagcgcaag tcagcgtcgt gcgtgagtcc cccacggtcg
     2401 acgcgctgat cgaggcgctg agggctgatg atccctcagc cgttcaaagc ggcctgcgtc
     2461 gggccgtcgc aacgctgggg ccgcgccagt tcatcaccga cgtggcgagc ggcctggtgg
     2521 agagagtggg ggaagagtgg gcgcagggcc ggctcggggt tcgtcacgag cacctcttgt
     2581 ccgaagcgct ttctacccaa ctccgtctgc ttctttcggc ttacgacaat gccggtgcgc
     2641 ccgtcgtttt gctgaccgca gtgagtcggg aacagcacag cttgggcctc gaaatggcgg
     2701 cgctttactt agcgctggag ggagcgtgcc cacggctgct gggcgtggat acaccaccgg
     2761 atcaaatcgt cgaagcggcg tccgcgctcg gtgctcgggt catcggattg agcatctcga
     2821 ccgcggcgga caccgaggtc gtctcggagc aagtgcgctg gatcttgacc gagctgccgc
     2881 cgggagtgga gctgtggttg ggaggcaaga ccgcggggcg tctcggcctc agtgacggtc
     2941 ggctctttta cacgcccacc tggcctgacg tcgatcgcga gctcgcccgt gtacggggtc
     3001 gaaactccgc cgtttcggcc gcctgacgcg cgtcagaatc gcgccggaac gctcggctcc
     3061 gtcactcgaa gacgacggtc ttgttgccgt agatgagcac gcggtcgttc aggtgcgccc
     3121 gcacggcgcg cgccagcacg accttctcta ggtcgcgacc cttgcgtacg agatcatcta
     3181 ccgaatcccg gtggctcgcc cgcaccacgt cttgttcgat gatggggccc tcatcgagca
     3241 ccgcggttgc gtagtgggcc gtcgctccga tcaacttcac gcctcgttca tgggcctggt
     3301 gatagggccg cccgccgatg aatgcgggca gaaacgaatg gtgaatgttg atgatgcgcc
     3361 ctgcgtagcc gcgcacgaag tcctcgctga gcacctgcat gtagcgcgcg agcacgatca
     3421 aatcggcgcc gatctcctcg attcgctgcc gctctagctg ctccgcttcg cgtttgttgg
     3481 acgcattctt ctcgatcacc tcgaacggca cgccgaaatc acgcgccacc ggcgccaggt
     3541 ccgggtggtt actgaggatg agcgggatct cgcagctgaa ctcgttggcg cgacgtcgca
     3601 gcagaagatc gtacaagcag tggtcgtact tggagacgaa cacgaccacg cgtttgggcc
     3661 gctccgcata gaccagccgg tactgcatcg agaagcgctg ggccacctcg gcgatcgctc
     3721 gctccagcgt gccccgatcc gtgagcagct ccgacaagtc gaaacggata cgttggaaga
     3781 actgctgctg gtgcagatcg gtgtgctgat ccgaatccag gatgtttgcg ccgtgcccgt
     3841 acagcacttg ggccaaagcc gcgacgatgc cccgacgatc ggggcacgag accaagagag
     3901 ttgccagatc cttcgccatg atggggagtc attcgcgccg gcgggcgcga tcattcaaca
     3961 gtgcgcgcca agcctcgatg cgatcgcggg cttgggcgct gaagcggtag cgcgacaaac
     4021 gcccgagtag atggggggtg tgatctccgg cgcgcgtgcg aggcccgaac acctcggcgg
     4081 cggactcgat cgattcgagc gcgcgatcga gctctccgcg ctcgcattcg agccaagccc
     4141 gcgtgagcaa caaatgttgg cgaggctccg cctcttcgat cggcacccgt tgcgagtctt
     4201 cgagcaaccg cacggcgcgc ctgagatcat ggcgcatccg ggccgcttgg cagagcagca
     4261 gcatggcttc cacgataccc caaggatccg aagcgcgcgt gaagacgcga tgcgaacgct
     4321 cggcgtggcg ctccgcgagc tccacgtcat ccgtgtccac cgcgatcatc gcgagcaggc
     4381 gatcgcacgc ggcttgacct cggggcgtac gcagcgtctc gaatacggtg agcgcttcga
     4441 gcgcaccccg ttcggcgcca gtgaagttca tcagccggtg ctccacgtgt gaaagcgaag
     4501 cgtccgcctg ggccgttccg aggcggtagc cggctcgttc gaactccgcc cgcgcttcct
     4561 gcgcgagccg ccgagagcgc tcggtcgctc cctccgagtg gtcgatccag ctgagcaaca
     4621 gcaagcactg tccacggccg agcggctgat ccagcgccgc gaagttccgc tcaccttgct
     4681 cgatggtggc gcgcgcctcc tcgtagctgc cgagcaggta ctggatctcc cccgctactg
     4741 cttggcagtg agcttggccc agcacgctgc cgagcgcctc gaacgtgcgc aaggcccgct
     4801 gcacgagctc caggccctct gcgttggagc cctgctcgct tgcgatatgg ccgagcagac
     4861 gcacgcagtg ggcgatgttt tcttggtcac cgagctcctc gaaacgcgtg cgcgccagct
     4921 ccgcgtaggt gctcgcttct gcggtgcgac ccacgtgacg caggcactcg gcttgccaac
     4981 gattcttcag cgctagcgtg cgcccgctga gtcggttctt gaacagctcg agatcatcca
     5041 aagtcgcgag cggctcgcgc gccccgttcc aactgttctg caggaagtcg aacaacaggt
     5101 acgcggccga atccgcgtct cccgcttgca tcaggttgac gacgcgctgc cgcacgatgc
     5161 ggcgagtgtt ggcgagcgga tgttgcgtca gcgcggccgc ggcggcgcgg tagatccgct
     5221 ccttgtcggg ccgttctccg agacgcagca gcaggtgctc ctgcagcagc tcgtggggcc
     5281 agctcaatcg gcccggcccg cgcgggatca gaatttccgc gttctgcagg cttaaaatcg
     5341 cggcatccgc cggctgtccc agggccgtga gcagcgcgaa caatacattg cgccgaatgt
     5401 ctccaccgag cgtcgccgca gcgtgggccg aagggcggtg cgcttcggga acggccgtca
     5461 gccgcgtatc ccaaagctcc gccgtcgtct cgggacgcac cgcgagcacg tccggcggca
     5521 cccgatagaa gttgtccttg agctccaggc tgcccgccaa agcccacgcg tgcagctgtt
     5581 gcagcgcgaa caacgggttg ccgcggctgc gacgagcagc ttcttgggcg gccgcatcgt
     5641 ccaacggcaa agacgcacgc agcagctcga gcgtcgtctc gttcgggagc gggtcgatct
     5701 gaatcacgac cccctgcatc gcgtcgcgca gctcgcgcag ccgttccgcg gtctgcgttc
     5761 cgagtgccac gtcctccgag cgcaccgttc cgaccatcac gatgcgttgg tccggatcat
     5821 ccgtcagcgt gcgcagcaga ccttcgaacg tcgtcggcgc cgcgttgtgc aaatcatcca
     5881 accaaaacag caatggccgg ccgttggcga tgcgccgcaa cgtgtagcgg atcaccatgc
     5941 ggcgcgtctc gagcgtatcc agcgtgaagc ggatgccgct cggaccgatg ggctgatcgc
     6001 tcatcggtcc catcgggcgg aaccactctg cggttcccgc tacccaggcg cggccattct
     6061 tgtcgtcgcg tcgcaccttc cagcggtcga gcagagaacg ttcgatggtg ttccgatcga
     6121 cgcgctcgaa gttgtagtag ttgacggccg cgcccagcat accgtcgagc gagctccgga
     6181 tctttcgata gcgcgagcgc agcggcacca tcgtgccctc ttcgtgcacg gtctcgcaca
     6241 accactcagc gatgcggctc ttgccgcacc cggccgggcc caccaacagc actaggcggt
     6301 gcggcgcacc gccgccttgg atgacctcat cgcatacggc gcgcagccgt tcgcgtacgt
     6361 cccgacgccc gacgagcggg ctcggacgaa tgctgagcaa ccccggagcg cgctccgggg
     6421 cgggcagcaa atcggggttc tgcgcgcgtg gcccggtcgg gcgtgtcttg gggtgctcac
     6481 tttgcgcgcg cgggggagtg gggaaaaacc aaaccccgcg ccgctcctcg ggacggaagc
     6541 gtgcccagtc gcggcggcat tccgccgcga attcccagcg atcccagggc cgcttggcca
     6601 gcaagcgcat gacgaacgct tccacgcccg ctggcacctc gaacacgggc gtgaacttcg
     6661 gcggcggctc gaacgcgtgc actcgcagga gctcacgcga ttctcccgag aagggcgccc
     6721 gtcccgcgat gatgcgatag agaatgcagc cgagcgcgta gagatccgtc gcgccgcaca
     6781 cgtagtgcat ctcgtgttgg atctgctcgg gcgccatgta gcccggagtt cccgcgcccg
     6841 catgcggcgc gaattccatg gccttggagc cgtcgagacg ctcgtcatgc cagtcctgac
     6901 gcagccacgc gaggccgaag tccagcacgt gtacgtcggg gggttcgtcg tcgcgccgct
     6961 ctacgaggat gttcgagggc ttgagatcgc cgtggatgat gccacgcgcg tgggcatgcg
     7021 cgagcgcgct caagatgcga tcggtgatcg cccacagcac cgggaactgc acccgagtgt
     7081 gggcgaggtc gtgcatggag atgccctgca cgagctccat caccaaatag ggtgagccgt
     7141 ccctcagctg cccgaagtca cgcgccttga cgatgttcgg gtggtccaag gcggcgagcg
     7201 cacgcgcctc cttgtagaac cacatcaggt attcgttcgc gagattgccg ggcggtggga
     7261 tgacgcgctt cagcgcgact tgatggccat tcgactgatc gcggcagcga tagacgatgc
     7321 ccatgccccc ttcgccgagc aggctcatta cctcgtagcg gctcgcgatg acctgccccg
     7381 gcaagacagg cgcacctgcc ggtgaggctt tggcgctctt cacctcggcc gccgagggaa
     7441 tgctgggagg cgcgctgtcc tcgtctttct cgggcaccgc cgacaagatc tcgacgtcat
     7501 tgaccgcgtt tggatcagtg cgtcgtgctt cctcggcggg agtcgccacg cgtggcttca
     7561 ccgctcggtt ggcggtgggc ggcttatgga cgggcggcaa cttgttcggg ggcaccgctt
     7621 gtgcagccgg cgcagcggcg ggaataggag ggggtgcggt aaccgctgcg ggtggagctg
     7681 cagcgggagc cgctgctctt tctgcgcgtg ctgcggtcgc aggttgaacc ggggccgcac
     7741 tttgcctctc ctccgaagct tcggcgggtt cctcctcggg gttcttggct tggctagcct
     7801 cggccatctc gcagcggccg agtgtagtcc cctcggcgtg gccgtcgaga gcctgcactg
     7861 ttaaacagtg tcctcgcgtt gcagtgtgcc ttcaaacccc aataggcgtt taattccgaa
     7921 cgtttgggaa tcactggcgc gagccggccg gtccgagtac cctctcaccc gccatggcca
     7981 ccgagagaga gctccgagat ccgcccttgt ccgtccgcga tccagagatc gcaaacctga
     8041 tcaagcagga agagctgagg gaaaccgtca agctgcgttt gatccccagc gagaactatg
     8101 cctcccgagc cgtgctcgaa gctacgggct cggtcctcac gaacaagtac tcggagggct
     8161 atgcgggcaa gcgctattac gaaggtcagc agctcatcga tcaagtcgag gagctcgcgt
     8221 gctctcgctt gaaagcgctg ttcggcgcgg accacgtgaa cgtgcagcct tacagcggaa
     8281 gcccagcgaa cctggctgtt tatctagcct tctgtcagcc gcatgatccg atcatggggc
     8341 tcgcgttgcc ggcgggtggc cacctcaccc atggttggaa cgtcagcatc actggcaagt
     8401 acttcaagag cgtgggctat ggcgtgaacc gaacggacca ccgcatcgac ttcgacgaag
     8461 tggcgcgcct cgcccgggag cacaagccga agctcctgtg gtgcggaacc acggcctatc
     8521 ctcgtatcgt agacttcgca aaattccggg aaatcgcgga cagcgtcggc gccaagttga
     8581 tcgctgacat cagtcatatc tcgggcctgg tcgtcggcgg cgtgcacccc tcccccgttc
     8641 ccgtcgcgga cgtcgtcacg agcaccacgc acaagactct gcgcggcccc cgcggcggta
     8701 tgatcctgtg caaagcggag cacgcgaaag acatcgatcg cgcggtgttt cccggtcttc
     8761 agggcggccc gcacaaccac acgacggcgg gtatcgccgt cgccgcgcgc gaagcggcca
     8821 cgccggagtt caagcagtac gccgaaaaca tcgtgcggaa ctcgaagacg ctggccgaag
     8881 cgttgctcgg tcagggcttc tccctagtca cggggggcac ggacaatcac ctgatcttga
     8941 tcgacctgac caacaagaac atcccgggca aggtcgccgc gcgtgccctg gatcgcgccg
     9001 gcatcgtctg caactacaac tcggtcccgt tcgatccacg taaaccgttc gacccgtcgg
     9061 ggatccgtat cggcacgccc tcgatcacct cgcgtggcat gggccccgcc gaaatgcagc
     9121 gcctggctgg ctggatgcag caagtcgtcg ccgcccccga caacgaagcc cagctggata
     9181 agatcgccgc tgagatcaaa gagatgtgca gcggcttccc gccgcccggc atccccgtct
     9241 gagcgcgggc actcgggaga agaacccatg actgaaacca cgtgttccaa gctgctcgcc
     9301 cgcgctgagc gtctgatgcc gggcggagtg aacagcccgg ttcgcgcatt caaagcggtg
     9361 gggggtggtc cgcctttcgt ggcccgagct cgcggctcgc ggctgtggga cgcggacggc
     9421 aaagaatacg tggacttcgt gggttcctgg ggcccggcga tcttgggcca cgcggatccg
     9481 cacgtggtgc aggccgtgca gcaggcggcc gaacgaggcc tatcgttcgg ggcaccgaca
     9541 gagctcgagg tgcggttcgc cgagcgcatt acgcgtctct acccgagcat cgagctcctg
     9601 cgctgtgtct cgagcggcac cgaggcaacc atgagcgcgc tgcgcgtcgc gcgcggcttc
     9661 acccggcgcg acctgatcgt caagttcgat ggttgttacc acggtcacgc cgacccactg
     9721 ctggtcaagg ccggcagcgg agcggccacg ttcggcaatc ccgattcggc aggcgtgccg
     9781 gctagcatcg tcgcgaacac ggccaccctt tcattcaatg acttcgaggc gctcgaatcg
     9841 ctgtttgcgg cgcgaggcgc ggagattgcg gcggtaatcg tggagcccgt ggttgggaat
     9901 atgggctgcg tgccaccgca
//
LOCUS       DASTKU010000190         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_258883, whole genome shotgun sequence.
ACCESSION   DASTKU010000190 DASTKU010000000
VERSION     DASTKU010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258883"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1786
                     /locus_tag="VFQ61_07190"
     CDS             <1..1786
                     /locus_tag="VFQ61_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HET9954270.1"
                     /translation="AVHEPVCNRHRVLLLEAELARVRGELVLATARYEAALSAARASG
                     MLHEEALCAEWAGRYFQSIDNPTVTRAYLSRARFLYERWGALAKLAQLDREFPDLAAT
                     ARAPVSATGPTWTGTGVLRASEHGLDLLSVVKASQAISGEIELTRVLSRLITIAVENA
                     GASRGLLIVERNRKLLIEAELSALDSTPVVGRSQPALESGKVPVSVLNYVVRARKPLV
                     LDEAADSGPFTSDPYLAVNRPRSILTIPLMSQGVMRGVIYLEHGAAPRMFTAERIEVL
                     SLLSTQAAISLENAGLYADLKSSLQEQVRLNEAHKRFVPHEFLHSLGRSSIVDVALGD
                     SVLKEMSILFSDMRSFTSLVEKLSPDQNIGFINTYLGHMEPEILKNGGFVEGYIGDAI
                     MALFEGGADGAVRAGSDMLRALERFNRERAARGERPCRIGIGINTGMLTLGTIGGNAR
                     IKCGVIGDSVNVAARVESITKRYGVALLISDDTYRRLAEPARHSIREIDRVRLAGRKS
                     VVTLYEVFDADARQLREHKLAMAEDYGAALEAYYARDFLGAIVRLGRCRSKLPDDPVV
                     LHHLARCERYKEQAPDADWDGVEELSHK"
     gene            1877..>2197
                     /locus_tag="VFQ61_07195"
     CDS             1877..>2197
                     /locus_tag="VFQ61_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954271.1"
                     /translation="MIPTLPDQDPDPVSRRAQLTRARETWRFLKRYSMGSFPGIGLAA
                     AVPVSDRFSPNYGLEVGRVGGAALLNHLLLSVTGRFAGNVRKPSAQDWLDVARARHRP
                     ESGLW"
BASE COUNT          404 a          681 c          710 g          402 t
ORIGIN      
        1 tgccgtacac gagccggtct gcaatcgcca ccgcgtgttg ttactggaag cggaattggc
       61 gcgcgtgcgg ggtgagctgg tgttagccac ggctcgctac gaggccgcac tctcggccgc
      121 tcgggcctcg ggcatgctgc acgaagaggc gctctgcgcc gagtgggcgg gccgctactt
      181 ccaatcgatc gacaacccca cggtgacgcg tgcgtatttg tcgcgggcgc gcttcctata
      241 cgagcgctgg ggggcgctcg ccaagctcgc gcagctcgac cgcgagtttc cagatttggc
      301 ggcgacagct cgagcgcccg tgtcggcgac gggtccgaca tggaccggca cgggagttct
      361 acgcgcgagc gagcacggtc tcgacctgct cagtgtcgtg aaagcgtccc aggccatctc
      421 gggagaaata gagctgacgc gcgtgttgag ccggctcatc acgatcgcgg tcgagaacgc
      481 cggcgccagc cgcgggctct tgatcgtgga gcgcaaccgg aagctgctga tcgaagccga
      541 actctccgcg ctagactcca cgcccgtggt cggccgctcg caacccgcgt tagagagtgg
      601 gaaggtgccc gtctcggttc tgaactacgt cgttcgcgcg cgcaaaccgc tggttctcga
      661 cgaggccgcg gactccgggc cattcaccag tgatccctac ctggccgtta accgaccgcg
      721 gtccatcttg accatcccct tgatgagcca gggcgtcatg aggggggtga tctatctcga
      781 gcacggggcc gccccgcgga tgtttacggc cgaacgcatc gaggttctca gcctcctctc
      841 gactcaagcc gccatttcgc tcgaaaacgc ggggctatac gcggatctga agtcatctct
      901 ccaagaacag gtgaggctca acgaggctca caagcgcttc gtgccacacg aattcttgca
      961 cagcctcggg cggagcagca tcgtcgatgt ggcactcggt gacagcgtgc tgaaagagat
     1021 gtcgatcttg ttctcggaca tgcgcagttt cacgagcctg gtcgagaaac tctctccgga
     1081 tcagaacatc gggttcatca acacttacct cggccacatg gagcctgaaa tcctcaagaa
     1141 cgggggtttc gtcgaaggct acatcggaga cgccattatg gccctcttcg agggcggagc
     1201 ggatggagca gtacgagccg gcagcgacat gttgcgcgca ctcgaaagat tcaaccggga
     1261 gcgtgccgcg cgcggcgaac ggccatgccg cattggaatc ggaatcaaca ctggcatgct
     1321 cacgctcggc accattggtg gcaacgcccg catcaagtgc ggagtaattg gagacagcgt
     1381 gaatgtcgcg gcgcgcgtgg agagcatcac caagaggtat ggggtagcgt tgctcattag
     1441 cgatgacact tatcgcagac tcgccgagcc cgcgcgccac tcgattcgcg agatcgaccg
     1501 tgtccgcctg gcaggccgga agagcgtcgt gacgttgtac gaggtgttcg acgccgacgc
     1561 tcgccagctc cgtgagcaca agctggcgat ggcggaggac tacggcgcgg cgctagaggc
     1621 ctactacgcg cgagacttcc tcggcgccat cgtccggctc ggccgctgtc gatcgaaatt
     1681 gccggacgac ccggtcgttc tccaccacct tgcgcgctgc gagcgatata aggagcaagc
     1741 gcccgacgcg gattgggatg gtgtcgagga gctatcccac aagtgattgg cggacggcaa
     1801 cggcttttgg cagagtcgcc aggcacctgc ttcagcgata tcaaccgctt caacagatca
     1861 actgttcggc aacacgatga ttccaacgct acccgaccaa gatcccgatc ccgtctcgcg
     1921 ccgggctcag ctcacgcgtg cccgagagac ttggcgtttt ctcaagcgtt actcgatggg
     1981 tagcttcccg ggaatcgggt tggccgcagc cgtgccggtg tcggatcgtt tctcgccgaa
     2041 ttacggtctc gaggtgggcc gggtcggggg cgcggcgctc ctcaaccact tgctgctcag
     2101 cgtgacgggt cgcttcgcgg gtaacgtacg caaaccgtct gcacaggatt ggctggacgt
     2161 ggcgcgtgcg cggcatcgcc cggagtccgg cctatgg
//
LOCUS       DASTKU010000191        14639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_705761, whole genome shotgun sequence.
ACCESSION   DASTKU010000191 DASTKU010000000
VERSION     DASTKU010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14639
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705761"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /gene="urtA"
                     /locus_tag="VFQ61_07200"
     CDS             <1..1265
                     /gene="urtA"
                     /locus_tag="VFQ61_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HET9954272.1"
                     /translation="AKADDTGPIKVGILHSLSGTMAISETSLRDVALMTIEKINESGG
                     VLKRKLEPVVVDPKSDWPMFAEKARELLQKEKVAVTFGCWTSVSRKSVLPVYEELNGL
                     LFYPVQYEGEESSYNVFYTGAAPNQQAIPAVEYLMSAKGGGAKKWVLLGTDYVYPRTT
                     NKILRYFLKGKGVSEENILEKYTPFGHSDYQTIVADIKKFAAGTPTAVVSTINGDSNV
                     PFYKELANQGLKASDIPVVAFSVGEEELRGIDTKALVGHLAAWNYFMSTDTPENKKFI
                     ADFKAYAKAHNLPDAEKRVTNDPMEATYIGMKMWAQAVEQAGTTNVDAVRQAIGYQVV
                     RSPSGFNIPMDAKNHHLHKPVFIGEIKADGQFQIVWKTEGPIRAQAWSPFIPESAKKV
                     ADWTFPWVCGNCTEPKFKSDTAVAVAAP"
     gene            1353..2975
                     /gene="urtB"
                     /locus_tag="VFQ61_07205"
     CDS             1353..2975
                     /gene="urtB"
                     /locus_tag="VFQ61_07205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HET9954273.1"
                     /translation="MQRAFHFLLGLMLAWVLAFSSDVHLARAQGRSDLRALMSETESQ
                     AAVEGLAARKDAFAERALSALLDGNLRIDAAGAGFIADSSGALTPLEPAGPKQPAQPV
                     STPSLNNQLRRVIEPVLAGLRLSSADRSVRLGAAEELSKRQSPELAPLIERALGTERD
                     GTVRQKLELALAQVRLQSSDTAQRLSALEIIRKAAEVSFKPELDRLVAKDETGAYREK
                     NEEVRRAANAALGAIETRAFIYSSIGNAFYGLSLGSVLLLVALGLAITFGLMRVINMA
                     HGELLMIGAYATHVVQKLFEAYLPAQVDWYILCALPVAFLASGLVGFAIERTVLRFLY
                     GRPLETLLATWGVSLILIQTVRLIFGAQNVTVANPSWLRGGVELLPGFVLTYSRLAVI
                     SFSAIVLLFVWFMLQRTPLGLHVRAVTQNRQMAASMGISTRRVDSLTFAIGSGVAGLG
                     GVALSQLGNVGPELGQAYIVDSFMVVVLGGVGKLAGTLAGAFGLGLVNKLLEPVAGAV
                     LGKIAILVFIILFIQRRPQGLFALKGRAAEVT"
     gene            2972..4234
                     /gene="urtC"
                     /locus_tag="VFQ61_07210"
     CDS             2972..4234
                     /gene="urtC"
                     /locus_tag="VFQ61_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HET9954274.1"
                     /translation="MTAPNPIANSSEHQSPDDRSTTDLASEPAPHSSPVPTRDAPSAA
                     ARESSSVPRSGLVGSAARAERLEPVMSPRQWLSWAIALVVGAVLIPILNSLPADSALH
                     LPDYLVTLFGKFCCYATVALAMDLLWGYTGILSLGHGVFFALGGYAMGMCLMLGIGAA
                     GVYRSNLPDFMVFLDWKELPWFWRPFDNFWFAMFAALAAPGLLAYAFGFFAFRSRIRG
                     VYFSIITQALTFAMMLLFFRNETGLGGNNGLTDFKQIYGYPLQKGSTKLALYMLSCAS
                     VFVTFAICRFVTTSKAGRVLRSIRDEENRVMFLGYDPTRFKLFAYTLSAVLCGLAGAL
                     YVPQVGIINPGELQPSNSIEMAIWVAVGGRGSLLGAPLGAFVVNGAKSIFTSLMPEAW
                     LYMLGLLFVSVTLFFPQGLLGVLSRLKK"
     gene            4234..5229
                     /gene="urtD"
                     /locus_tag="VFQ61_07215"
     CDS             4234..5229
                     /gene="urtD"
                     /locus_tag="VFQ61_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HET9954275.1"
                     /translation="MASETADSKASPNSKAAESKAEPKAAESKAPPSPRRAALSLVPR
                     KRTVEKERYHALTHGRKALLCVDSISVSFDGFRALNDLTLVLDEGELRCIIGPNGAGK
                     TTLMDVITGKTKPDTGSVFLMGDETDLTQLNEYEIAERGVCRKFQRPTVFHGHTVFEN
                     LELALPGKKGVFASLFSKLGGEQRDRIAKILATIGLEEQSKVRAGLLSHGQKQWLEIG
                     MLLAQDPRVLLVDEPVAGMTQAETERTAELLNSLAGQHTVVVVEHDMEFVRSIAKHVT
                     VLHEGHVLKEGSMDEVQNDPKVIEVYLGESSTTLSPKASAANAGVGAGALASEGA"
     gene            5232..5924
                     /gene="urtE"
                     /locus_tag="VFQ61_07220"
     CDS             5232..5924
                     /gene="urtE"
                     /locus_tag="VFQ61_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HET9954276.1"
                     /translation="MLEVKDLNQYYGESHTLWDMNLSVPEGSCTCLMGRNGVGKTTLL
                     KSLMGLLPVRSGSIKLQDRDLAKEPPEARARHGVGYVPQGRDIFGQLSVMENLRTGLA
                     ARADKATQVPERVFELFPVLRDMLKRRGGDLSGGQQQQLAMGRALALGPKLLILDEPT
                     EGIQPNIVSQIADVLSELRSQGLTILLVEQKLPFARRVADRFCIMDRGRRVASGEMKE
                     LDDTMVAKYLTV"
     gene            complement(5948..6766)
                     /locus_tag="VFQ61_07225"
     CDS             complement(5948..6766)
                     /locus_tag="VFQ61_07225"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9954277.1"
                     /translation="MQRSSFSAGCLFSLLLSASCGGSGKPEAATQEQVVNSRAGSKAR
                     AARAAEPELDPGQIVLGLRGHEADLRACFDRNDAPQPAFVELAFNVNPEGEPRDIRES
                     RSLAAAPEIIECLSENLATARFGRPGRTLAARWTFVGGLVRFEDAEARRRKRGPHKRD
                     RGEVQEREVVIEESSPGKLDPDKVQSIVQSGYKLFAHCYRDGLDRDPKLGGAVRLRFV
                     IGAAGSVARVIDGGSDIPDPGVVTCIAESFFALRFPKPAGGNVHLRYRIHFDAS"
     gene            complement(6843..7853)
                     /gene="galT"
                     /locus_tag="VFQ61_07230"
     CDS             complement(6843..7853)
                     /gene="galT"
                     /locus_tag="VFQ61_07230"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET9954278.1"
                     /translation="MSILRFDVTTGDWLAYSSSRAHRPDGFGRVALSAPPAVAREPHC
                     PFCPGNEALTPATIDVDPHPDDAESWCVRIVANKYPVLEPSASTLRRLAGPLFREMDG
                     HGRHELVIETERHDLALPDQSPEHVLRLLRVLHRRSQALSRDPTLEVVQIFKNHGAVA
                     GSSMPHPHFQILATPVLPREIRLKHETAAEYYNVAGVSVYTDLCRAELEVGTRIVSIN
                     DEFVAFAPYASRVPYETWILPRKAAPTFESADVMSLPLLAEILKQVLGRMKRALNDPP
                     YNLVVNSAPRRHADEPDFVWHIEVLPRLTTTAGFELATGMAINTVLPEQAAEVLRATA
                     PI"
     gene            8041..9849
                     /locus_tag="VFQ61_07235"
     CDS             8041..9849
                     /locus_tag="VFQ61_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9954279.1"
                     /translation="MNRYVVLRALILALLIIGLTRCHRREAPQASAFPRKETLYVGGL
                     MWGEPTTFNPLTPIPSWPVNDGNLIYETVFVFDALSGKMVPSIGESCEITDEYVEVTL
                     NAAARWNDGQPLTARDIKYNFDLGKRFKGLRSGPLWTYITEIVLPEEGHLAPGQQPRR
                     LRFMLNQQEKNPLLVLDQLVERYIVPEHVFEPLLASLNFNIEALLKLKFDKNPVGSGP
                     YRLHSYSSEKVVAIRDDNYWGNAALHQGKLPAPKYVIHPIYKSNDHFSVALQQGRIDI
                     STAYMPRIWLKRKKGVRSWFEEEPFFLPACIPMMFLNTQHAPLNDVRVRRAMAFAVNY
                     DDIRELAVSGYSPKIKSGLILPFGLESKYFSEEDAQKYGASRYDPEAARALLREGGYQ
                     PIFNEKGELVETRDAQGKKLPTLFIKSPTGWSDWESIVRIAVRGMRAVGIDVRERFVD
                     AAIFWQSKYVGDFDLIMDSPMPQPYPSKPWTRFENVLTTRSYAPLGQKMFKNYGRFNN
                     PSGSDYEPRVDELLKLIPTLKDQAAIAAAYRELNVLFMKLQPTLPMVYRGDQLYQFST
                     RVWQGFPTAKDPYLPPQLPGYKLGTRILWHLRPAVP"
     gene            9849..10925
                     /locus_tag="VFQ61_07240"
     CDS             9849..10925
                     /locus_tag="VFQ61_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9954280.1"
                     /translation="MFENHPTLRHILGRAAWYVVTFLVAVTINFFLPRLGDASPIDVL
                     MARATAGSSVDLRTARDREEAYLKEFGLVEVDSSGQIRRDPAGKPVRTTLIRQFTSYL
                     GMSLRGDLGTSILQHPKRVSEIIANALPWTIALQLPTIGLGWVIGNLLGALAAYRRGV
                     FDKVLYPLALLANAVPAFCFGILLVYLFGIQLEWFPAVGGYDEGLTPSLSWDFFSSAS
                     YYYVLPFSSVFLIWVGGQAIGMRSMCIYELGTDYVKYAKLLGISERRILLYMFRNAML
                     PQLTGLALALGAMVGGALVTEMVFSYPGLGMAVLTAIQSNDYPVITGCTLLVTVCVLI
                     ANFSVDVLVGFLDPRIRAAQTGGK"
     gene            10930..11766
                     /locus_tag="VFQ61_07245"
     CDS             10930..11766
                     /locus_tag="VFQ61_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9954281.1"
                     /translation="MKLLKGLLKSPAFLGGATLLLLTLLLAFVVPLFIQVDTQTRFGL
                     PYQAPSRAHWLGTDHMGNDMLSLLIVGLRSSLHVGFLAGSVATLLGTLIGVYGGYKGG
                     LLDDVLTVVTNLFLVIPSLIVLILLSSSLEQGRSLSLIALIIGCTTWTWSARAVRAQA
                     ASLRNRDHVHLARINGYTTFDIVVVHILPYLLSYVFMVFILQTATAILSEASISMLGL
                     GPYDSISLGRILNEAIRNEALVDGAWWAFAPAMALVTVVAFALYVINTSMEGVFNPRL
                     RK"
     gene            11772..12746
                     /locus_tag="VFQ61_07250"
     CDS             11772..12746
                     /locus_tag="VFQ61_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9954282.1"
                     /translation="MSLVLEVEDLRVHYLTRFGERVQAVDGVSFNLNAGEVLGIAGES
                     GCGKTTLVSGCLGLYIPPLHHTSGDVRIAGQSIIGMSPQQNRKQVLGQKIAMIPQGAL
                     NSLNPTRKVKDMAADMILSHDESASRKNIYERLRERFSRIGLDADTVLNSYPVHLPAG
                     TKQRVVIGISTLLNPKVVIADEPTSALDVSTQKSVIRLLFELMDQDIIASMVFITHEL
                     PLLRHVSSSIAVMYAGQFVEIGSTEQIIFDGRQPYTRALMGSMLSAEPGQRQRKPVAI
                     EGAPPNLANPIPGCRFAERCPVVRPDCPSTLQELRPVAGRLARCRYAE"
     gene            12736..13596
                     /locus_tag="VFQ61_07255"
     CDS             12736..13596
                     /locus_tag="VFQ61_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HET9954283.1"
                     /translation="MRSSDHPEGRVSGAAGRDDPSTRPCKFEARALTRSYGHGKKIAR
                     AVDAVSFEIRDREIVSIVGQSGCGKTVLGKLLLRLERPSSGTLLFNGRPIDAEVDARV
                     HWRQVQAVFQDPFSAFNQFFTIRSQLKSSFRLFPNKLPEVEMEARIDEALLNVNIKPR
                     DIDGKYPFELSGGQMQRMLLARIFILQPQVLIADEPTSMVDACSRATILDYLMDLKRK
                     LDMTIVFITHDIGLAYYVSDSLFIMHEGRIVEQGPPDRVITAPASEVTRRLLDDIPNV
                     HRDWLGRKTS"
     gene            complement(13593..14087)
                     /locus_tag="VFQ61_07260"
     CDS             complement(13593..14087)
                     /locus_tag="VFQ61_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HET9954284.1"
                     /translation="MRVMVVEGDLLEQDVEAIVNAWNRNLFPWWLLLPQGVSGAIKRR
                     AGTLPFRELRRHGLLAPGGAVLTGAGRLEFKAIIHVAGIGLLWRASRHSIQESVRNAV
                     ALAAGHSFRSMAFPLIGAGTGGVHPELAEAIMIETLRALSFDGEIRVVRYRGGHSVRG
                     RVAY"
     gene            14167..>14639
                     /locus_tag="VFQ61_07265"
     CDS             14167..>14639
                     /locus_tag="VFQ61_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9954285.1"
                     /translation="MNPIKARLLFYGAVLLVSTAAVAYTTAMPGRSFSGPLPEPTSEL
                     KLLEGRLRGHVNALATDIGVRNWEHAAQLAAARDYIRSVLAPLESAEARTTLEDLGSA
                     GQGGQNVVFEISGAEATSLLVVGAHYDSAHVSPGANDNASGVAAALELARSLARAP"
BASE COUNT         2737 a         4461 c         4545 g         2896 t
ORIGIN      
        1 ctgccaaagc ggacgatacc ggcccaatca aggtcggtat tttgcattct ttatcgggca
       61 cgatggcaat cagcgagacc tcacttcgag acgtcgcgct gatgacgatc gagaagatca
      121 acgagtccgg cggtgtattg aagcgcaagc tcgagccggt cgtcgtggat ccgaagtcgg
      181 attggccgat gtttgccgag aaagctcggg agctgctcca gaaagagaaa gtcgcggtta
      241 ccttcgggtg ttggacctcg gtctctcgta agagcgttct gccggtctac gaagagttga
      301 atggcctgct cttctatccg gttcagtacg aaggggaaga atcctcttac aacgtgtttt
      361 acaccggcgc ggcgccgaac cagcaagcca ttcccgcggt ggaatacctg atgagcgcca
      421 aggggggcgg cgccaagaag tgggtcctgc tcggtacgga ttacgtttat cctcgcacca
      481 ccaacaagat tctacgctac ttcctgaagg gcaagggtgt gagtgaggaa aacatcctcg
      541 agaagtacac ccccttcggt cacagcgatt atcaaacgat cgttgccgac atcaagaagt
      601 tcgcggctgg tactcccacc gcggtggtct ccacgatcaa cggtgactcg aacgttccct
      661 tctacaagga actcgcgaac caaggtttga aggcttccga catcccggtg gtggcattct
      721 cggtcggcga agaggagctg cgcggtatcg acaccaaagc gctcgtcggt cacttggcgg
      781 cttggaacta cttcatgtcg acggatacgc cggagaacaa gaaattcatc gccgatttca
      841 aggcctacgc caaggctcac aacttgccgg atgccgagaa gcgcgtcacc aacgatccca
      901 tggaagcgac gtacatcggg atgaagatgt gggctcaagc cgtggagcag gcgggcacca
      961 ccaatgtcga cgccgtgcgc caggcgattg gatatcaggt cgtgcgctcg ccatccggtt
     1021 tcaacattcc gatggacgcc aagaaccatc acctgcacaa gccggtgttc atcggcgaga
     1081 tcaaggcaga cggtcagttc cagatcgtgt ggaagacgga ggggccgata cgagcccagg
     1141 cgtggagtcc gttcatcccc gagagcgcca agaaggtcgc ggattggacg ttcccctggg
     1201 tgtgcggcaa ctgcacggag cccaagttca agtccgatac agcggtcgcg gtcgccgcac
     1261 cctgaggcgc cgattcgtcg agctacgggt ctcgtgcgcg ggacccgtag cctcactccc
     1321 aagcaattcc acgaataaag ttacggtgtc cgatgcagcg cgcattccac ttcttgctgg
     1381 ggctgatgtt ggcgtgggtc ctggcgttct cgagcgacgt gcacctcgcg agggctcagg
     1441 gtcgcagtga tctccgcgcc ctgatgtccg agaccgaatc gcaggcagcc gtcgaggggc
     1501 tggccgcgcg caaagatgcg ttcgccgagc gcgccctctc cgccctgctc gatggcaatt
     1561 tgcgcatcga cgctgcgggc gcaggcttca tcgcggattc gagcggggct ctcacgccgc
     1621 tcgagccggc ggggccgaag cagccagctc agcccgtctc caccccgagc ctcaataacc
     1681 agctgagacg ggtgatcgag ccggttctcg ccggtttgcg gctcagctcc gccgatcgat
     1741 cggtgcggct cggtgccgcc gaggagctca gcaagcggca gagcccagag ctcgcgcccc
     1801 tgatcgaacg cgcgctcggc accgaacggg acggaaccgt gcgacagaag ctggaactcg
     1861 cgctggcgca ggttcgactc caaagcagcg acacggcgca acgcttgtcc gcgctagaaa
     1921 tcatccggaa ggccgccgaa gtcagcttca agcccgagct ggatcggctg gtcgccaagg
     1981 acgagaccgg cgcctatcga gaaaagaacg aagaggtgcg ccgcgccgca aacgctgcgc
     2041 tcggcgcgat cgagacccgc gccttcatct actcgtcgat cggcaacgcc ttttacggcc
     2101 tgagcctcgg cagtgtgctc ttgttggttg cgttgggact cgcgatcacc tttgggctca
     2161 tgcgcgtgat caatatggct cacggcgagc tgctgatgat cggggcttac gccacgcacg
     2221 tcgtgcaaaa gctgttcgag gcctatctgc cggctcaagt ggactggtac atcctgtgtg
     2281 ccttgccggt cgcgttcctc gcttcgggct tggtcgggtt cgccatcgaa cgcacggtgc
     2341 tgcgattcct gtacgggcgc cccctggaga ccctcctcgc aacctggggt gtctcgctga
     2401 tcttgattca aaccgtgcgc ctgattttcg gtgctcaaaa cgtgaccgtg gccaacccga
     2461 gctggttgcg gggcggcgtg gagcttctgc ctggcttcgt gctcacgtac agtcgtttgg
     2521 cggtcatctc cttcagcgcg atcgtgctgc tgttcgtgtg gttcatgttg cagcgcactc
     2581 cgctcggttt gcacgtgcgc gcggtgacgc aaaaccggca aatggcggcg agcatgggta
     2641 tctccacgcg acgggtggac tcgctcacgt tcgcgatcgg ttccggagtc gccggcctcg
     2701 gcggggtggc gctctcgcag ctgggcaatg tcgggcccga gctcgggcaa gcctacatcg
     2761 tggattcgtt catggtggtg gtgctcggcg gagtgggcaa actcgcgggt accctggccg
     2821 gggcattcgg gctggggttg gtcaacaagc tgctcgagcc ggtagccggc gccgtactcg
     2881 gaaagatcgc catcttggtg ttcatcattc tgttcattca gcgccgcccc caaggcttgt
     2941 ttgctctcaa gggacgcgcc gcggaggtca catgaccgct cccaacccga tcgcgaactc
     3001 atccgaacac cagtcaccgg acgatcgatc gacgaccgac ttggcgtcag aacccgcacc
     3061 gcacagcagt ccagtcccga cgcgcgacgc gccctcggcc gcggcccgtg agtcctcgtc
     3121 ggttccacgc tccggcctgg ttggaagtgc ggcacgggcg gagcgactcg agccggtaat
     3181 gagtcctcgc cagtggctga gttgggccat cgctctggtc gtcggagcgg tgctgatccc
     3241 cattctcaat tcgttgccgg ccgactcggc tttgcatctg ccggactact tggtgacgct
     3301 gttcggcaag ttctgttgtt acgccaccgt ggccttggcg atggacttgc tctggggcta
     3361 caccggcatc ttgagcctcg ggcacggcgt gttcttcgcg ctcggcggct acgccatggg
     3421 catgtgcctg atgctgggca tcggggcggc cggcgtatac cgcagcaact tacccgattt
     3481 catggtgttc ttggattgga aggagttgcc ttggttctgg cgcccgttcg ataacttctg
     3541 gttcgcgatg ttcgccgcgt tggcggcgcc cgggctcttg gcatatgcgt tcggcttctt
     3601 cgcgtttcgc tcgcgcattc gcggtgtgta cttctcgatc atcacccagg cgctcacgtt
     3661 cgcgatgatg ctgttgttct tccgcaacga gacgggcctg gggggcaaca acggcctcac
     3721 tgacttcaaa caaatttacg gttacccact gcagaagggc agcaccaagc tcgcgctata
     3781 catgctgtct tgtgcgtcgg tgttcgtcac cttcgcgatc tgtcgcttcg tgaccacgtc
     3841 caaagccggc cgcgtgctcc gctccatccg cgacgaagag aaccgcgtca tgttcttggg
     3901 ctatgacccg acccgtttca agctgttcgc ctacacgcta tcggcggtgc tgtgcggctt
     3961 ggctggtgcg ctctacgtgc cgcaggtagg tatcatcaac cccggtgaac tccagccctc
     4021 gaactcgatc gagatggcga tttgggtggc cgtgggcggc cgcggcagct tgctcggagc
     4081 gccgctcgga gcgttcgtgg tcaacggcgc gaagagcatc ttcacctctt tgatgccaga
     4141 ggcttggctc tacatgttgg ggttgttgtt cgtgtccgtg accctgtttt tcccgcaagg
     4201 tctattgggc gtcttgtcga ggctgaaaaa gtgatggctt ctgaaaccgc cgactccaaa
     4261 gcttccccca actccaaagc tgcggaatcc aaagccgagc cgaaagccgc cgagtcgaag
     4321 gctccgcctt cgccgcgcag ggccgcactg agcttggtgc cgcgcaagcg taccgtcgaa
     4381 aaggagcgtt atcacgctct cacacatggc cgaaaggcgc tgctctgcgt cgacagtatc
     4441 tcggtcagtt tcgacggctt cagggcactc aatgatctaa ccctcgtgct ggacgagggc
     4501 gaactgcgct gcatcatcgg cccgaacggg gcaggtaaga ccacgctgat ggacgtgatt
     4561 accgggaaaa ccaagcccga cacgggctcg gtgttcttga tgggtgacga gaccgacctg
     4621 acacagctca atgaatacga gatcgcggag cgcggagtgt gccgcaaatt ccagcgcccg
     4681 acggtgtttc acgggcacac cgtgttcgag aatctagagt tggcgctacc cggtaagaag
     4741 ggagttttcg ccagtctctt ctccaagctc ggaggggaac agcgcgatcg gatcgccaag
     4801 atcctcgcta cgattgggct cgaagaacag tccaaagtcc gcgcaggact actctcgcat
     4861 ggccaaaaac aatggctcga gattggcatg ttgctcgcgc aggatccgcg ggtgttgctg
     4921 gtagatgagc ccgtggcggg catgacgcag gccgaaacgg agcgcaccgc cgagctcttg
     4981 aactcactcg cgggacagca caccgtggtc gtcgtcgaac acgatatgga gttcgtgcgc
     5041 agcatcgcca agcacgtcac ggtgctgcac gaagggcacg tcttgaaaga aggctccatg
     5101 gacgaagtgc agaacgatcc gaaggtgatc gaggtctacc tcggcgaaag cagcactact
     5161 ctgtccccca aagcgtccgc cgcaaacgcg ggcgtcggcg ccggtgcgct cgccagtgag
     5221 ggagcttgag catgttggag gtcaaagatc tgaaccagta ttacggcgag agtcacacgc
     5281 tctgggacat gaacttgtct gttcccgagg gctcctgcac ctgcttgatg ggccgcaacg
     5341 gcgtcggcaa aaccacgcta ctcaagtcct tgatggggct cttgccggtg cgctcgggca
     5401 gtatcaagtt gcaagaccgc gatctcgcca aagagccgcc ggaggcacgg gcccgccacg
     5461 gcgtcggata cgtgccccag ggtcgagaca tcttcggcca actgagcgtg atggagaatc
     5521 tgcgcacggg gctcgccgcg cgcgcagaca aggccactca ggtgccggag cgagtgttcg
     5581 agctgttccc cgtactacga gatatgctca agcgccgtgg cggcgatctc tcgggtgggc
     5641 agcaacaaca actcgcgatg gggcgcgcgc tcgcgctcgg acccaagctg ttgattctcg
     5701 atgagccgac cgaggggatt cagcccaaca tcgtgtcgca aatcgccgac gtgttgagtg
     5761 agctccgcag ccaaggccta acgatcctgc tcgtcgaaca aaaactgccc tttgctcgtc
     5821 gagtcgccga ccgcttctgc atcatggacc gcggacgtcg cgtcgcatcc ggcgagatga
     5881 aggagctcga cgacacgatg gtggccaagt acctgacggt ctgatccgcc cccggcgacg
     5941 aagggcccta gctggcgtcg aagtggatgc ggtaacgcag gtgtacgttg ccgccagctg
     6001 gtttcggaaa gcgcagcgcg aagaagctct cggcgatgca ggtcactact cccggatcgg
     6061 ggatgtccga gccgccgtcg atcacgcggg ccacgctgcc tgccgcacca atcacgaagc
     6121 gcagccggac cgcgcctccg agcttgggat cgcgatcgag cccatcgcgg tagcagtggg
     6181 cgaacagctt gtatccggac tgcacgatgc tctggacctt gtccggatcg agcttgcccg
     6241 gcgaagactc ctcgatcacg acctcccgct cttggacctc gccgcgatct cgcttgtgag
     6301 gccctcgctt gcgccgtctc gcttccgcgt cttcgaagcg caccaagccg ccgacgaaag
     6361 tccaacgcgc ggccagggtg cgtccggggc gcccgaagcg cgctgttgcg aggttctcgc
     6421 tcaagcactc gatgatttcc ggcgcggccg ccagcgaccg gctctcgcga atgtctcgcg
     6481 gttcaccctc ggggttgacg ttgaatgcga gctctacgaa ggccggttgc ggcgcatcgt
     6541 tgcgatcgaa gcaagcacgt aggtccgctt cgtggccgcg caatccgagc acgatttgcc
     6601 cgggatcgag ctcgggttcg gctgcccgtg ccgctcgtgc tttggacccc gcgcgtgagt
     6661 tgacgacctg ctcttgcgtt gccgcttccg gtttcccgga gccgccgcag ctcgcgctca
     6721 gtaacaacga gaataggcag cctgcgctaa agctcgatcg ctgcatgggt accatccagg
     6781 gccgttaggt atagctcgtt cgaccgcgag tcctgcggaa aacgccgcag gatctggcta
     6841 cttcaaatcg gagccgtagc gcgcaggact tcggccgcct gttccgggag caccgtgttg
     6901 atggccatgc cggtcgcgag ctcgaacccg gccgtggtgg tgaggcgcgg caagacttcg
     6961 atgtgccaaa cgaaatcggg ttcatcggcg tgccgacgcg gcgcgctgtt caccaccagg
     7021 ttgtacggtg gatcattcag cgcgcgcttc attcgcccga gcacttgctt gagaatttcc
     7081 gccagcaggg gcaggctcat cacgtcggcg ctctcgaaag tgggcgccgc cttgcgcggc
     7141 aagatccaag tttcataggg cacccgcgag gcgtaaggcg cgaacgccac gaattcatcg
     7201 ttgatgctca cgatgcgcgt tcccacttcg agctcggccc gacacaagtc ggtatacacg
     7261 ctcactcccg caacattgta gtattctgct gccgtttcat gcttgaggcg gatctcgcgg
     7321 ggcagaacgg gggtggccag aatctggaag tgcggatggg gcatcgacga gccggctacg
     7381 gcgccgtgat tcttgaagat ctgaaccacc tccaaggtcg ggtcgcggct gagcgcctgg
     7441 gatcgccgat gcaacacccg cagcaaccgc aacacgtgtt ccgggctttg gtcgggcaga
     7501 gccaaatcat ggcgctcggt ctcgatcacc agctcgtgtc gcccatgtcc gtccatttct
     7561 cggaacagcg gccctgcgag ccgacgcaag gtcgatgcgc tcggctcgag cactgggtat
     7621 ttgttggcga cgatgcgcac gcaccagctc tcggcatcat cgggatgggg gtccacgtcg
     7681 atcgtggcgg gtgtgagagc ctcgttcccc gggcaaaacg ggcaatgcgg ttcacgtgcg
     7741 acggccgggg gagcggagag cgcgacacga ccaaagccat ctggtcgatg cgccctgctg
     7801 ctcgagtaag ctagccaatc gccggtggtg acgtcgaaac gcagaatgga catggcgcct
     7861 tcacaggcta ttccgacacg gcgccgggcg cgaccggaac ccaaaaaact cgaggaatgc
     7921 gggtctcggg gtgcgcagat gatcgctgat atcgcggcgt gttcgctggg catggggttc
     7981 acgcccggcg cttgacaggg ctgacgccgt cgcctaactc tgcctcaacc cccgacaacc
     8041 atgaaccggt acgtcgtgct gcgcgcactc atcctcgcgc tgttgattat cggtctcacc
     8101 cggtgtcacc gccgcgaagc tccccaagcc tctgcctttc cacgcaaaga gacgctctat
     8161 gtgggcggat tgatgtgggg tgaaccaacc accttcaatc cgctcacgcc gattccgagt
     8221 tggcccgtca acgacggaaa cctgatttac gaaaccgtgt tcgtgttcga cgcgctatcc
     8281 ggcaagatgg ttccgtcgat cggcgagagt tgcgagatca cggacgagta cgtggaggtg
     8341 acgctgaatg cggccgcgcg ctggaacgac ggtcagccac tcaccgcgcg cgacatcaaa
     8401 tacaacttcg atttgggtaa acgcttcaaa gggctgcgct cggggccgct ctggacgtac
     8461 atcaccgaga ttgtcttgcc ggaggagggc cacctggctc cgggacaaca gcctcggcgc
     8521 ttgcgcttca tgctcaacca gcaggagaag aacccgctgt tggtgttgga ccaattggtc
     8581 gagcgctaca tcgtgcccga gcacgtattc gagccgctgc tcgccagctt gaatttcaat
     8641 atcgaggcgc tgctgaagct caaattcgat aagaacccag tcggctccgg gccttatcgt
     8701 ctgcactcct actcgagtga aaaggtcgtc gcgatccgag acgacaacta ctggggcaac
     8761 gccgcactgc accagggaaa gctccccgcc ccgaagtacg tcattcaccc gatctacaag
     8821 agcaatgacc acttcagtgt cgccctgcaa cagggacgca tcgatatttc gacggcctac
     8881 atgccgcgca tttggctcaa gcgcaagaag ggggttcgca gctggttcga agaagaaccc
     8941 ttcttcctcc cggcttgcat cccgatgatg ttcctgaaca cgcagcacgc accgctgaat
     9001 gacgtgcgcg tgcggcgggc catggcgttc gcggtgaact acgacgacat tcgggaactc
     9061 gcggtgtcgg gatatagtcc gaagatcaaa tccggcctga ttctgccgtt cggactcgaa
     9121 tccaaatact tctccgagga agatgcccag aagtacggcg ccagtcgtta cgatcccgaa
     9181 gcggcgcgag cgttgctgag ggagggaggt tatcagccca tcttcaacga aaagggcgaa
     9241 ctcgtagaga cccgggacgc ccagggcaag aaactgccga ccttgttcat caagtcaccg
     9301 accggttgga gtgattggga atccatcgta cggatcgcgg tcaggggcat gcgcgcggtg
     9361 ggaatcgacg tgcgagaacg cttcgtcgac gcggccatat tttggcaatc gaagtacgtc
     9421 ggcgacttcg acctaatcat ggatagcccc atgccgcagc cgtatccttc caaaccgtgg
     9481 acccgcttcg agaacgtgct gaccacgcgc agctacgccc cgctcggcca gaagatgttc
     9541 aagaactacg gacgcttcaa caatccgagc ggctccgact acgagccgcg ggtcgatgag
     9601 ctgctcaagc tgatccccac gttgaaggat caagccgcga tcgccgcggc ctatcgggaa
     9661 ctcaacgttc tgttcatgaa gctccagccc acgctgccca tggtctaccg cggggaccaa
     9721 ttgtaccaat tcagcacgcg agtatggcag ggcttcccga ccgccaaaga cccttatctg
     9781 cctccgcagc tacccgggta caagctcggc acgcgcattc tttggcacct ccgcccggcg
     9841 gtgccctgat gttcgagaat cacccgaccc ttcgccatat cctcgggcgc gccgcttggt
     9901 acgtcgtgac ctttctggtt gcggtgacca tcaacttctt tctgccgcgc ctgggggatg
     9961 cgagcccgat cgacgtgttg atggctcgcg cgacggctgg ctcgagcgtc gatctaagaa
    10021 cggctcggga ccgtgaggag gcctatctca aggagttcgg cctggtcgaa gtggattctt
    10081 ccggacagat ccgtcgagac cccgcgggta agccggtgag aactactcta atccggcagt
    10141 tcaccagcta cttgggcatg agcttgcgcg gcgatctcgg cacgtcgatc cttcagcatc
    10201 cgaaacgagt gagtgagatc atcgccaatg ctctgccgtg gacgatcgcc ctgcagctgc
    10261 cgacgatcgg cttgggttgg gtgattggca atctgctagg ggccttggcc gcgtatcgca
    10321 gaggggtatt cgacaaggtc ctctatccgc tggctctgct cgccaatgcg gtcccggcgt
    10381 tctgttttgg gatcctgctc gtttacctat tcggcattca gctcgagtgg tttccggcgg
    10441 tggggggcta cgatgagggg ctcacgccca gcttgagctg ggattttttc tcgagtgcaa
    10501 gctactacta cgtgctgccc ttctcgtcgg tatttctaat ctgggtgggt gggcaggcca
    10561 tcggcatgcg ctcgatgtgc atctacgagc tcgggaccga ctacgtgaag tacgccaagc
    10621 tgctagggat cagcgagcgg cgcattctgc tgtacatgtt tcgcaacgcg atgctgccgc
    10681 agctcaccgg cttggctttg gcgctcgggg caatggtggg cggggcgctc gtaacggaga
    10741 tggtgttcag ttatcccggg ctcggcatgg cggtgttgac cgcaattcaa agcaacgact
    10801 acccggtgat aacgggttgc acactgctcg tgacggtctg tgttttgatt gcgaacttca
    10861 gcgtggacgt gctcgtgggc ttcttggacc cacggatccg cgccgcgcaa acgggaggca
    10921 agtgagctcg tgaaattgct caagggcttg ctcaagtcgc ccgccttctt gggtggagcc
    10981 acgttattgc tgttgacgct gctgctggcc ttcgtcgtgc cgctctttat ccaggtggac
    11041 acgcagacgc gattcggttt gccctatcaa gccccatcgc gggcgcactg gctcggcacc
    11101 gatcacatgg gcaatgacat gctctcgctg ctgatcgtgg ggctccggtc gtccctgcac
    11161 gtgggtttct tggcgggcag cgtggccacc ctgctcggca ccctgattgg agtgtacggc
    11221 ggctacaaag gtggcttgtt ggatgacgtg ctgaccgtcg tcaccaacct gttcctggtg
    11281 attcccagcc taatcgtact gatcctgcta tcttccagct tggagcaggg gcgcagcttg
    11341 tctctgatcg cgttgatcat cggctgcacc acctggacgt ggagcgcccg tgcggtgcgg
    11401 gcccaggccg ccagcttacg caatcgggac cacgttcatt tggcgcgcat caacggctac
    11461 accaccttcg atatcgtggt cgtgcacatc ttgccatatc tgctctcgta cgtgttcatg
    11521 gtcttcattc tccagaccgc gacggccatc ctttcggaag cgagcatcag catgctgggg
    11581 ctgggcccgt atgattccat ttcacttggc cggattctga acgaggcgat tcgaaatgaa
    11641 gcactggtcg atggggcttg gtgggcgttc gcgcccgcca tggccctggt gaccgtggtc
    11701 gcgttcgctc tctacgtcat caacacctcg atggaaggtg tgttcaatcc tcggctgagg
    11761 aagtgagccg catgagcctc gttctcgaag tcgaagattt gcgggttcac tatctgaccc
    11821 gcttcggtga gcgcgtgcag gcggtggatg gggtttcttt caacctgaac gcgggtgagg
    11881 tgttgggtat cgccggggag tcgggctgcg gtaagaccac gctggtgagc ggctgcttgg
    11941 ggctctacat tcctcccctg catcacacgt cgggggacgt ccgcattgcc gggcaaagca
    12001 tcatcggcat gagcccacaa cagaatcgta aacaggtact cgggcaaaag atcgccatga
    12061 tcccgcaggg cgcgctgaac tcgctcaacc cgacccgcaa agtgaaggac atggcggccg
    12121 atatgatctt gagccacgac gagagtgcct cgcgaaagaa catctacgaa cgtctgcgag
    12181 agcgcttctc gcgcatcggc ctcgacgcgg acacggtctt gaatagctac ccggtgcatt
    12241 tgcccgccgg caccaagcag agggtcgtca tcggcatctc cacgctgctg aaccccaaag
    12301 tggtgatcgc ggacgagccc acgtcggcgc tggacgtatc gacccaaaag tcggtcattc
    12361 gcctgctgtt cgagctgatg gatcaggaca tcattgcgag tatggtgttc atcacccacg
    12421 agctaccatt gctgcgccac gtatcgagtt cgatcgcggt gatgtatgcg ggccaattcg
    12481 tcgaaatcgg gagcacggag caaatcatct tcgacggtcg ccaaccctac acccgcgcgc
    12541 tgatgggttc gatgttgagc gccgagccag ggcaacggca gcgcaagccc gtggcgatcg
    12601 aaggtgcgcc gccaaacctg gccaacccca tccccggttg tcggtttgcc gagcgttgtc
    12661 cggtcgtgcg tcccgattgc ccgagcacac ttcaagagct gcgtcctgtc gccgggcgac
    12721 tcgccaggtg ccgttatgcg gagtagtgat catcccgagg ggcgggtgtc tggcgctgcg
    12781 gggcgagatg atcccagtac gcggccctgc aagttcgagg cgcgggcgct cacacgcagt
    12841 tacggacacg gcaagaagat cgcgagagcg gtcgatgccg tcagcttcga gatccgggac
    12901 cgcgagatcg tatccatcgt cgggcaatcg gggtgcggta agaccgtctt gggcaaactg
    12961 ctgttgcgac tcgagcgccc gtccagcggt acactgctgt tcaatggccg tcccatcgac
    13021 gccgaggtgg acgcccgcgt ccactggcgc caggtgcagg ccgtatttca agatcccttt
    13081 tcggctttca atcagttctt cacgatccgc tcgcaactca agagctcgtt tcggctcttt
    13141 ccgaacaagc tgcccgaagt ggagatggag gcgcgcatcg acgaagcctt gctgaacgtc
    13201 aacatcaagc ctcgcgacat cgacggaaag tatcccttcg agctctccgg gggacagatg
    13261 cagcgcatgc tcttggcgcg catcttcatc cttcagccgc aggtcttgat tgcggacgag
    13321 cccacgtcga tggtggacgc ctgcagccgc gcgaccatcc tcgactactt gatggacctg
    13381 aaacgcaagc tcgatatgac catcgtgttc atcacccatg acatcgggct cgcatactac
    13441 gtttcggatt cactcttcat catgcacgag gggcgaatcg tagagcaggg cccgccggat
    13501 cgagtcatca cggctcccgc gagtgaggta acgcggcggt tgttggatga cattccgaac
    13561 gtgcaccggg actggcttgg acgcaagacg tcctagtagg cgacccgacc ccgaaccgaa
    13621 tgcccgcctc ggtaacgaac cacgcggatc tcgccgtcga atgagagcgc gcgcagggtt
    13681 tcgatcatga tggcttcggc aagttctggg tgcaccccac ccgtccctgc gccgatgagc
    13741 gggaatgcca tggaacggaa ggagtgtcct gccgccagag cgaccgcatt tctgacggat
    13801 tcttgaatgg agtgacgcga ggctctccaa aggagtccga tcccggcgac gtgaatgatg
    13861 gccttgaact cgagtctacc ggcgcccgtg aggaccgccc cgccgggcgc caagaggccg
    13921 tgacgccgga gctcccggaa cggaagggtg ccagcgcgcc gcttgatggc cccggatacc
    13981 ccttggggga gcagcagcca ccaagggaac aggtttcgat tccaggcgtt gacgatagct
    14041 tcgacgtcct gttccagtaa gtccccctcg acaaccatca cccgcatgag caggtaacgc
    14101 cgctgagaaa aaggtcattc caaagcttcg agaatccaaa gtgtaacaac ggcgttggct
    14161 catgacatga acccgatcaa ggctcggctg ctgttctacg gcgcggtgct gttggtttcg
    14221 accgcggcag tggcctacac cacagcaatg ccgggacgct ccttttccgg tccgttgccc
    14281 gagccgacgt cggagctgaa gctcctcgag ggacgcttgc gagggcacgt caatgctctc
    14341 gctacggata ttggcgttcg caattgggag cacgcggccc agttggcggc cgctcgggac
    14401 tacatccgtt ccgtcttggc cccgctcgaa tctgccgagg cacgtaccac tctcgaggat
    14461 ttgggcagcg ccggccaagg cggacaaaac gtggtgttcg agatttcggg cgctgaggcg
    14521 acatcgctgc tcgtggtggg tgcacattac gattcggcgc acgtgagccc tggcgccaat
    14581 gacaacgcct caggcgtagc cgcggcactc gaattggctc gttcgctagc gcgcgcgcc
//
LOCUS       DASTKU010000192        21439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1822305, whole genome shotgun sequence.
ACCESSION   DASTKU010000192 DASTKU010000000
VERSION     DASTKU010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 21439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21439
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822305"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VFQ61_07270"
     CDS             <1..1197
                     /locus_tag="VFQ61_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954286.1"
                     /translation="YIVREMQAPEGGYYSATDADSEGEEGKFFVFLPEEIDDILGKDD
                     AEAFCLYYDISVGGNFEGKNILNTPRSVAEVARELGVDAGGLARELEIARQKVYAARE
                     QRPKPLLDDKILTSWNGLMIEAMAEGYRVLRNPLYLESATRAADFVLRQLARPDGGLF
                     RTTRAGRTHVEAFLEDYAYFGDALVSLYEASGQFRFLEHSRRLAERVMRDFSAEDGAF
                     YQTAAQHEALITRARSGHDGALPNANAVTARWLARVGYHYDSQDLVERARRALLGYGQ
                     LVERAPRMFATALCVTQWLLDGPTELVLVGDPGQPNTERFLEELSRRYLPNRVVAMFP
                     DRSPPPTRLARDKSALHGRPTLYVCRNFACLSPLTEPSHVAAALQQDRERPQAPDRLE
                     LAPVRA"
     gene            1265..2692
                     /locus_tag="VFQ61_07275"
     CDS             1265..2692
                     /locus_tag="VFQ61_07275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9954287.1"
                     /translation="MSRARRVLLVEPDERKRVVLAERLKAQNFAVEAFGDPAEAANFA
                     LSKPPHALVANLWMPGVSGVQMCRLLKAEPATLDVPIILRAEGDAPRARFWAEQAGAV
                     AYVPTGRIGELVRALERAISETPTAEGFFMHLPQADIRDRIAQHLDRALFESVIASSV
                     RALGTCESFARLFDLFSQFVCRITTYRWLALRMDQPVRLALHCHPSNVDASLAAAQHT
                     LGACATEPWVVEDEDAKDIETSCPVVSHDVGIGEQKLGTLAIQPLDNESDVEELVRLM
                     ATEIAGPLKISVLVEESRRLAQHDPLTGIMNRRAFTERMHAELEQPFDRQANLYLLLL
                     DVDHFKAINDTCGHAGGDRVLFEVGRLLTRCLPARGHLARWGGEEFVLAFRTSSSGAA
                     LEFADELRESIANHTIRLEDSASINVTVSIGVAEHLNEEPLESLIERADHAMYAAKVA
                     GRNRVVVAERTLRPAATPSLRVAAG"
     gene            complement(2738..3205)
                     /locus_tag="VFQ61_07280"
                     /pseudo
     CDS             complement(2738..3205)
                     /locus_tag="VFQ61_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(3221..5023)
                     /locus_tag="VFQ61_07285"
     CDS             complement(3221..5023)
                     /locus_tag="VFQ61_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9954288.1"
                     /translation="MLVSAFRRMAEILLLEDDDEMREVLAAVVAAEGHRVLESATIAD
                     ALERLTQATFDLAILDGQLPDGDGIDFARHLCLIQPQVKVIFLSSAWRDAKATNALRK
                     LGVTEIIHKPVPPVELALRVARVLRSRTGLPAALSSAPERSAPQAFAPSRASAPPQAS
                     ASSPVLSGHSRDDDGPALRAKPPDSERPTSSPPPVIPVTSSVPVSPAPLAERLTGLRL
                     TYSERLTGKLTELADTLKQARARDGQRHMIERAEYLAHRLRGTAGTYGFMSVSLAAAS
                     IESALQVLQRSGDAEVVWRRIDEALQRAEHAQRTPPSSVMPAIRRQAMARVLVVDDDP
                     ECLAQLEVLGRAVSLDMIPTSDAAQALEIARREPIDLALVDMCLKGDETPFQLVKDLR
                     GVDGRESLPVACMSANGSIKNRVAAVHAGATLFFEKPIDADELSAFTRRMLEAREAER
                     ARILILDDDPEAARGLARLLRGAGWLATTLSDVDRLFEVLGQVQPDLLLLDVMMPISG
                     FDICKALRASPTWQELPVVFLTGATDRRTVVACYGVGGDDYVGKPILPEELLARVRMR
                     IERSRMFRQRAHEDSLTGLLTRAAFSDVVEQRLA"
     gene            complement(5036..5257)
                     /locus_tag="VFQ61_07290"
     CDS             complement(5036..5257)
                     /locus_tag="VFQ61_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954289.1"
                     /translation="MFGVIHALWGESAGAGRCSHGRPTATGMEHLRFLQNLTAAVRSA
                     SAIQASEIWRELTYASFVFWPFAAALGAT"
     gene            5387..6997
                     /locus_tag="VFQ61_07295"
     CDS             5387..6997
                     /locus_tag="VFQ61_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9954290.1"
                     /translation="MAPDSLTSGEAAPDEAARLRAVHQLELLDTPPEERFDRITRLAA
                     QTLHTPIALVTLVDRERVWVKSRHGSRIREIARLGSFCSETLAHDEEFSVPDALADPR
                     FCEHELVRSEPHVRFYAGRSLRSSNGFRVGTLCILDRVPRRLTPDQGRALADLAFLIE
                     RELALGDRMRLKNEFVATVSHELRTPLTSIRGSLGLLLGEALGRLPEPVQSMLQIAHS
                     NSERLMRIVNDLLDIDKIEAGKLDFVLSRASLQPILERSLIENGELARARGVEFRLHM
                     GAADACARIDPDRLLQVLTNLLSNAVKFSPPGAAVELELEPRSEQVRIAVKDHGPGIP
                     HEFRARMFGKFAQADASDARSRGGSGLGLNIARALTERMGGQIGFTSELGLGSTFFVD
                     LPRCGALDHEHSIFLPRVLVCEADLDTARTLAIDLEAHGFIADLVSDPSAVQRALRDR
                     SYSALTLDLRLPESSEVLRTLRDDPRTRDLAVVVVSADVTDAASQRAAVGLEIAGWLE
                     KPFGPGELVRQLRAALARREHGAPRLTA"
     gene            7002..7397
                     /locus_tag="VFQ61_07300"
     CDS             7002..7397
                     /locus_tag="VFQ61_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9954291.1"
                     /translation="MAQELKRILIVEDDEDIRVIARIALEAVGGFEVESCETGAKAAL
                     LARTFRPHLVVLDVTMPDMDGPTTLRAMRKEPGLELTPVIFLTAKHHPQELRELEQLG
                     ALGVIQKPFDAMRLPSHIQQLWKRRFHTE"
     gene            complement(7401..7862)
                     /locus_tag="VFQ61_07305"
     CDS             complement(7401..7862)
                     /locus_tag="VFQ61_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954292.1"
                     /translation="MIRSFDRNELRIALGALLAAALIYAWPSFAVRRSTKMEIAITVV
                     PPDARGLQCRSPRPVVGARCGVGEGNLRPYVTLGGKLVLLAGVFEDPAVAAWYEQAKS
                     HPRERVRVICAGERLGAVDDVEVRFGPDQPFKKSGQISAARVKTCRIERDR"
     gene            complement(7859..9286)
                     /locus_tag="VFQ61_07310"
     CDS             complement(7859..9286)
                     /locus_tag="VFQ61_07310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9954293.1"
                     /translation="MTGQDLAAGVVDGPPLDNEGRGGADRVKRDPEEPVEPTGTTRAG
                     LQMRASDLLLVALVVLGLVCRLRDFSLPREFLFDEHHFVNNARNYLAGQADQNDHPPL
                     GKLLIAASIALFGDNSAAWRAPALLLGAASLGLAAFAAARSFKSPRAGWIALGLCAAD
                     GFLIAYSRAALLDGYLMCVGLGLLCVVSFAASPVTALAAGALLGLALAIKFSGVALLL
                     PILVWLAFTKGTWSKRAALIALVLVTAVSLYCGIYAFGLHTARQPATLSFVWFETKRL
                     YLHHSELTDMKNPATSGWSTWWLPVRPLMLGYGDDMGSVRALSSLGNLVAWWSSVALS
                     LGVIGVIAWFGLRAVLSDSGEARRASLPGALADQPLALELSSFVFQRSRGLLRTLALA
                     VGFVAPWVLTHRDSYIYHFLPAYAALLLILAGFLAHAEVKHRAAVLWFLVALLLVAAF
                     YAPVWSFAELAPSQLRARLFLGSWR"
     gene            complement(9377..10606)
                     /locus_tag="VFQ61_07315"
     CDS             complement(9377..10606)
                     /locus_tag="VFQ61_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9954294.1"
                     /translation="MSSERPRSFSLPPELCAELESLGLNETTAPDSARWQELLLRLGG
                     MIRARSFGRDGTALSHELRTPMTVVIGASELLLETELDADQRAAVQGVHRSGQELLAI
                     LNEVLDEPRESGRPSDVPRSRDHALRPDSRLDASVLVVEDNEFNRALITHALAGVGCR
                     VEQASTGMEALSKLAMDDYDVVLMDCHMPGLDGFETTRQIRMRERAPRRMPVIAVTAG
                     GTSGTRRACLDAGMNDYVTKPFSLATLRSRVAYWVARSRDASNVAAETPLTVPAPASA
                     DNQHLDLSRLEELADEAGSASIALELTRIFLGDIGKRVHALLVAVKQRDKVAVLSLAH
                     AIKGSAGNFGAVGMASFAESIERRYKENHLEKLDEDAEALGRELIMVQSLLDQHGLIE
                     RPNVPSMPMLRVSMRPR"
     gene            complement(10603..12027)
                     /locus_tag="VFQ61_07320"
     CDS             complement(10603..12027)
                     /locus_tag="VFQ61_07320"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9954295.1"
                     /translation="MDEEPQAPVTPANGELVAVVDDDPTARRLMRFWLERVGYRVVEH
                     ESGESAIRDEGESPNVACVDLGLGEVGGFKVIQHLRARDADLPIIVVTAQRELETAVA
                     AMRAGAYDYVTKPLDRDRLLLAVHRARERRELVNNVRHLENALGERGVLGSIVGQSPK
                     MRELAQQVERVLSSDVAVCVFGESGTGKELVARAIHNGSNRRRGPFVAINCAGIPESL
                     QESELFGHERGSFTGATQLRRGCFEQAHGGTLLLDELGEMSLTTQASLLRTLQEKTVR
                     RVGGSTEVPIDVRIVCATHRDLRAEVEAGRFREDLYFRLVVYPIHLPPLRERLEDIPL
                     LVGHFLRTLEVGAERKIDRVSPEALDALMHYRWPGNVRELQNVVHRSLLACRGEEIAL
                     ANLPVDIRNAVLPPLPPVNGKAAPEAQREEEIIPLRELERRAIQRALRATQGSVGKAA
                     KLLGIGRATLYRRIATLDLSQDVA"
     gene            12226..12987
                     /locus_tag="VFQ61_07325"
     CDS             12226..12987
                     /locus_tag="VFQ61_07325"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9954296.1"
                     /translation="MTAKKPFLRALPTEFGLLRVSGATWAEASDKTSINVMIDDHAEL
                     CAVLLEVHPQTDRAAVPSEGSSPAALLAEVQDLVLEALTQRAPLYEVVSSTRKFCAAH
                     AGCEVGIALMRLSQRDSRAEILNAGMPAIVWIAPDAEIVLHPALSGRIGHTPGEVHAY
                     ELCPLVWGSSWLMMSESFVLAQPAHTTSQEGTPGSERFDAQSWLSEQDVAYDAHLLAR
                     ETRERLCQRMHSVDGPVPADDAALVCIAASSEPRF"
     gene            13079..13615
                     /locus_tag="VFQ61_07330"
     CDS             13079..13615
                     /locus_tag="VFQ61_07330"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HET9954297.1"
                     /translation="MRIRTKTIARLRDALLESGRRPTLVTSSAYETLARQGLLSPEEL
                     TALARVEPVAELMFLMMAADGNVHELERDALRGAIRGLAGDTLRSGIIQVMLESFEER
                     LRNQGRLQRLHELAEELSEERADAESAFTLAAAIALADDHVADAENEFLNQVAEWLGL
                     STERANQVLDQLEQDAAE"
     gene            complement(13649..14701)
                     /locus_tag="VFQ61_07335"
     CDS             complement(13649..14701)
                     /locus_tag="VFQ61_07335"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET9954298.1"
                     /translation="MRAVSVVPSEVPPTSIPTASREIVFVAGGDVSFGREAGQRLLQD
                     PEYAPLAGIERLWDDADLRFVNLESTLSDQQGQTQSPLQRLIFTGPPSGARALGRAGV
                     HWVSTANNHAWDYGPGAFEQTLVNLDAAGVDHAGTGPDLAAAYAPIVRRVGGWSVALF
                     AVTHVWNFGPIDRHEARARVAWASFKALEPILRAAREAYDVVIVSYHGGAEYQDAPSD
                     LTRNFMKSVMSTGVDLVVGHHPHVLQGVGFFDGRPVFYSLGNLLFGRRKEHPWTRYGL
                     LARVSIAEEGERSFAVCPYRIDDWEPQAELSDAERSEVLHHLRLASTATGGLSLGATD
                     ARGCVAIAPALRRSRR"
     gene            15089..16408
                     /locus_tag="VFQ61_07340"
     CDS             15089..16408
                     /locus_tag="VFQ61_07340"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET9954299.1"
                     /translation="MKIAVIGTGYVGLVAGACFADSGTNVVCVDVDEQKLEKLRRGDV
                     PFFEPRLAELVKRNWPHRLDFSSNLAESIRDREVVFVAVGTPPNEDGSADLSHVLTVA
                     EQVAKAATHDLALVLKSTVPVGTNERVTEVVKRYAKHRISVVSNPEFLKEGDAVSDFF
                     KPDRVVIGTDDDHAFEALARLYAPYNRQRSRIQRMSPKSAEIVKYAANALLAVKISFM
                     NEIAKLCDVAGGDVEQVRVAVGSDERIGMQFLYPGLGFGGSCFPKDLRALVRTGEELG
                     VKLELAAAAVRANEVPVRTLLSHMEADLGGVAGKTIAVWGIAFKPRTDDVREAPSLKL
                     IQELVARGAKIRATDPQALETGGDRLRQLGITDNVQLFQKEYDASEGADALVIATEWN
                     EYRNPDLGRLKELMRGRFIFDGRNALTADAVADAGFVYRGMGRPRRN"
     gene            complement(16528..17412)
                     /locus_tag="VFQ61_07345"
     CDS             complement(16528..17412)
                     /locus_tag="VFQ61_07345"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET9954300.1"
                     /translation="MASLESPSSNRLKLSNPDADVFVPDGAALDAALTRTTYLGIGAH
                     ADDLEIFAAHGILAGFQSETEWFAGVVVTDGRGSSRGGPYAELSDAALREVRRREQRK
                     AAYVGEYAAQLQLDFQSASLRDGGSGARRALVADLEQILRITRPRVVYTHNLADAHAT
                     HVVVALAAIEACKRLEPERRPTQVWGCEVWRDLDWLSGADRKVLPLSSRSHVQQALLG
                     VFDSQIAANKRYDLATQGRRQANATFDAAYSADATSAATLAMDLLPVVEGADAREYVA
                     RLIERFKQDALDRLRDLG"
     gene            18046..18528
                     /locus_tag="VFQ61_07350"
     CDS             18046..18528
                     /locus_tag="VFQ61_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954301.1"
                     /translation="MNSTTLATAGGEQAGDANPGQGGDAPAGSVAGESGERGTSPAGA
                     GGTSPSQATNDGGTLSDGGTLSDGGAAGSQSTAQCTPNRARCTANVLQTCNSDGAWSE
                     PEECAAATPVCFEGACHACAEGQKRCSENLVQTCNASGNWETTQSCTLGCTAGKCLLL
                     "
     gene            complement(18534..19748)
                     /locus_tag="VFQ61_07355"
     CDS             complement(18534..19748)
                     /locus_tag="VFQ61_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnH family di-heme enzyme"
                     /protein_id="HET9954302.1"
                     /translation="MPKSKRYSKPSSTIATAGHFGSVLGLALTGALTGTTGCGSGQTG
                     EPTAYEWKLPAGFPKPKVPQENPMSAEKAELGRRLFYDKRLSSNETQSCASCHEQRLA
                     FSDGRANAVGATGVVNRRSSMSLTNAAYNSAQTWANPVLKTLEMQVAVPLLGDDPVEL
                     GFSGREFELVDRLKSDARYPELFRAAFPDEQDPLNLYNIVRAIASFERRLISGNSPYD
                     RYAAGEEGDAISESAQRGAKLFFGERFECHHCHGAFAFSNAATWEGKVGDQLFYQNDA
                     LYNVDGVGAYPARDQGVYEVTGKAEDMGRFRPPTLRNVAVTAPYMHDGSIATLREVLE
                     LHYARGGRLIAEGPDAGDGARSPLKSAFVSGFTIEAREIDDLLAFLESLTDESFLSDP
                     SLGDPFAETPTR"
     gene            complement(19714..20610)
                     /locus_tag="VFQ61_07360"
     CDS             complement(19714..20610)
                     /locus_tag="VFQ61_07360"
                     /inference="COORDINATES: protein motif:HMM:NF042462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnP family copper-binding protein"
                     /protein_id="HET9954303.1"
                     /translation="MRSISTIHVSLVARYALLGFAAAALVGCGDDESKDSSIYRNVEI
                     QFDLRVNGAPFECGARYPSVGSPPTEFSISDARLYLHNFELMDGSGETSPVELEENAF
                     QSDGLALLDFEDGCGSDGTPETHTTVSGRVVVPSTGSADAWSEVRFSLGVPDRKNFVD
                     LASAAPPLDVTGMFWIWQYGYKFLKVDGAASSLDGAIHSFILHVGASGCPGDKPQRPP
                     TGACERPNRASYRLPLRSGKIGLDLGELFATSDLSFNTEGTAPGCMSEPDDPECSELL
                     PRLGIAESTESDAKEQALFKAE"
     gene            complement(20824..>21439)
                     /locus_tag="VFQ61_07365"
     CDS             complement(20824..>21439)
                     /locus_tag="VFQ61_07365"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HET9954304.1"
                     /translation="SGPGFADTNQEIVFGVGHGCDGSDTQGIRIEIPAEVVSVRAVDA
                     TLGPATIELDDAGLVRFVSWQKPALSVLSSDSNYYKVSLRVKVPNRPFSTIYFPTRQT
                     CLSAEGEELIVDWASTEPSESESGPEPAPALKILPKRYPGWNKFQVSSAIEDLSAYFA
                     DAVIVWRGNSAYSSNPTTTELIAETSGVSTLGALASGDEIWVKY"
BASE COUNT         4080 a         7153 c         6581 g         3625 t
ORIGIN      
        1 tacatcgtgc gcgaaatgca ggcgcccgaa ggcggctatt attcggccac cgacgccgat
       61 agcgaaggtg aagagggcaa gttcttcgtc ttcttgccgg aggagatcga cgacattctg
      121 ggcaaggacg acgcggaggc gttctgcctg tattacgaca tttcggtcgg cggcaatttc
      181 gagggcaaga acattctgaa cacgccacgc agcgtagcgg aagtggcgcg ggagctcggc
      241 gtggacgcgg gaggcctcgc acgcgagctc gagatcgcgc ggcagaaggt gtacgccgct
      301 cgagagcaac gaccgaagcc cctcctcgat gacaagatct tgacctcgtg gaatgggctg
      361 atgatcgagg ccatggccga gggttaccgc gtcctacgca atcctttgta tctcgagtcg
      421 gcgacgcgtg cggctgactt cgtgttgcgg cagctcgcgc gccccgacgg gggacttttc
      481 agaacgacgc gggccgggcg aacgcacgtc gaagcgtttt tggaagacta tgcctacttc
      541 ggagatgcct tggtctcact ctacgaagcg tccgggcagt tccgcttctt ggagcacagc
      601 cgccggcttg ccgaacgggt gatgcgggat ttcagcgcag aggacggcgc cttctaccaa
      661 accgcagccc agcacgaagc gttgattacc cgcgctcgct ccgggcacga cggcgcactt
      721 ccgaacgcca acgcggttac cgcccgctgg ctggcccgcg tcggctatca ctacgactcg
      781 caagacttgg tggagcgcgc gcgtcgggca ttgctcggct acggacagtt ggtggagcgc
      841 gcgccgcgca tgttcgcgac cgcgctttgc gtgacacagt ggctgctcga tggcccgacg
      901 gaactggtgc tggtgggtga ccccggacag ccgaacacgg agcgattcct ggaggagctg
      961 agccgtcgct acttacccaa ccgtgtggtg gctatgttcc ccgaccgcag cccgccccct
     1021 acgcggctgg cgcgagacaa gagcgcgctg cacggtcgcc ccaccctgta cgtctgccgg
     1081 aacttcgcgt gcttgtcgcc gctcacggag ccaagccacg ttgccgcggc tctgcagcaa
     1141 gaccgtgagc ggccgcaggc gccggaccgg ctcgaacttg ccccagtgcg agcctaggaa
     1201 aattgctggc aagctcgcgg ggagactact gcgacgagca gcagggccgt tttctggagc
     1261 acagatgtcg agagctcgac gcgtgctcct agtggaaccg gacgaacgca agcgtgtcgt
     1321 tttggcggaa cggctcaaag cgcaaaactt cgcggtggaa gcctttgggg accccgccga
     1381 agccgccaac ttcgcgcttt ccaagccccc tcacgcgctg gtcgcgaacc tgtggatgcc
     1441 tggtgtgtcc ggtgtgcaga tgtgtcgcct gttgaaggcg gaacccgcca cgctggatgt
     1501 cccaatcatt ctccgcgccg aaggtgacgc tccccgcgcg cgcttctggg cggaacaagc
     1561 cggtgcagtg gcttatgtac ccacgggcag gattggtgag ctggtacgcg cactggaacg
     1621 cgcaattagt gagaccccta cggccgaggg gttcttcatg cacctcccgc aagcggacat
     1681 tcgtgaccgc atcgcgcagc acctggatcg cgcgctgttc gagtccgtga tcgcgtcttc
     1741 ggtgcgtgcg cttggcacct gtgagtcttt cgcgcgcctg ttcgacctgt tctcgcagtt
     1801 cgtgtgccgc atcacgacct atcgttggct cgcgctgcgc atggatcagc cggtgcggct
     1861 cgctttgcac tgtcacccct cgaacgtcga tgcctcgctg gccgcggccc agcacacgct
     1921 gggtgcctgc gcgacggaac cctgggtcgt cgaagacgaa gacgccaagg acatcgagac
     1981 cagctgcccc gtcgtcagcc atgacgtcgg catcggtgaa cagaagctcg gcaccctggc
     2041 catccaaccc ctcgacaacg aaagcgacgt cgaggagctg gtgcgcctga tggccaccga
     2101 aatcgcgggg ccgctcaaga tctcggttct ggtcgaggaa tcgcggcggc tcgcgcagca
     2161 tgatccgctc acgggcatca tgaatcggcg cgctttcacc gagcgcatgc acgcagagct
     2221 cgagcagccc ttcgatcgcc aagcgaatct gtatctgctg ctgctcgacg tcgatcactt
     2281 caaagccatc aacgacacct gcggtcacgc gggcggtgat cgcgtactct tcgaggtggg
     2341 ccgcctcctg acacgctgcc tccccgctcg aggtcacctg gcgcgttggg gcggtgaaga
     2401 gttcgtgctg gcgttccgga cctcttcgag cggggccgcc ttggagttcg cggacgagct
     2461 gcgcgagagc atcgcgaacc acaccatccg actggaagac tctgcgagca tcaacgtgac
     2521 ggtctcgatc ggcgttgcgg agcacctgaa cgaggagccc ctcgagagct tgatcgagag
     2581 agccgatcac gcgatgtacg cggccaaggt cgcggggcga aaccgcgtcg tagtggccga
     2641 gcgcacgctg cgcccagcgg ccacgccgag tctgcgcgtg gcggccggct agtcgccggc
     2701 caatcgccgg cagcggatgc gctgaacccg cgcgggttca gctcgacgcg cggaaggaca
     2761 ggcggctcgg cgtcgctggc gcgcgcgccg ccgaggttgg gtcgtcttgg tgttcgatgt
     2821 gattcttgcc ctcgcgtttg gcgcgataca gacgcgcgtc cgcgcgacgc atcaaatcct
     2881 ccaagctctc gccatcggca gggaaagaag cgaccccggc gctgaaggtc accgagaacg
     2941 gctccccgcg atcactctcg aagatcatct gtcgaaactc cccgagcacc cggtcgagca
     3001 ccgccgtcgt ggtctcggcg ttttcctcgt atagcgcgat cacgaattct tcaccgcccc
     3061 aacgcccgcg cacgtcctcc gttcggaaac ggctgcgcaa gaggcggccc aggccccgca
     3121 gcacccgatc tcccgccagg tgcccgtggt tgtcgttcac gtgcttgaat tgatcgagat
     3181 cgatcacgcc gagtgacagt ggacgatccc gacgcgaagc ctaggccagt cgctgttcga
     3241 cgacatccga aaacgcagct cgagtgagca aacccgtcaa actgtcttca tgggcgcgct
     3301 gccggaacat ccgtgagcgc tcgattcgca tgcgcacgcg ggccagaagt tcttccggca
     3361 agatgggctt acccacatag tcgtcgccac cgacaccgta acaagccacc acggtgcgcc
     3421 gatcggtcgc cccggtgagg aacacgaccg gcagttcctg ccacgtgggg ctcgcgcgca
     3481 gcgccttaca aatgtcgaag ccgctgatcg gcatcatcac gtcgagcagc agcaagtccg
     3541 gctgaacctg cccgagcact tcgaatagcc gatcgacgtc cgacagagta gtcgcgagcc
     3601 agccggcgcc gcgcagcaga cgcgccaatc cgcgcgcggc ctcggggtca tcatcgagaa
     3661 tcaggatgcg cgcgcgctct gcctcccggg cttcgagcat gcgccgcgtg aacgccgaga
     3721 gttcgtcggc atcgatcggt ttctcgaaga acagggtggc acccgcatgt accgccgcga
     3781 cgcggttctt gatgcttccg ttcgcggaca tacaggccac cggcaagctc tcgcggccgt
     3841 ctacgccccg caagtctttg accagctgaa acggcgtttc atcgcctttc agacacatgt
     3901 ccaccaaggc caaatcaatc ggctcgcgac gtgcgatctc cagcgcctgc gccgcgtcgc
     3961 tagtggggat catgtccagg gagaccgctc gaccgagcac ctcgagttgc gccaaacact
     4021 cggggtcatc gtccaccacc aagacgcgag ccatggcttg ccgccgaatg gccggcatca
     4081 cgctggacgg gggcgtgcgc tgcgcgtgct cggcgcgctg caaagcttcg tcgatgcgcc
     4141 gccacacgac ctctgcatct cccgagcgtt gcagcacttg aagcgcgctc tcgatactag
     4201 cggcggccag gctcaccgac atgaagccgt aggtgccggc ggtccccctc aagcgatgcg
     4261 ctaggtattc ggcgcgctca atcatatgtc gttgcccatc gcgggcgcgc gcctgcttga
     4321 gggtatcggc cagctcggtc aattttccgg tcaagcgctc gctgtacgtg agcctcagcc
     4381 cggtaaggcg ctccgcgagc ggcgcggggc tcaccggcac cgaactcgtg acggggatca
     4441 cgggcggcgg gctactcgtc gggcgctcac tgtccggagg cttcgctcgg agcgcaggcc
     4501 catcatcatc tcgcgagtga ccagagagaa ccggggaaga agcagaggcc tgaggaggag
     4561 cagaggcacg ggaaggcgca aaggcctgag gagcggagcg ctccggcgcg gagctcaggg
     4621 ccgccggcaa acccgtgcgc gagcgcagca cgcgcgcgac gcgcaacgct agttccacgg
     4681 gcggaaccgg tttgtgaatg atctcggtga cccctagctt acgcagagca ttggtcgcct
     4741 tcgcgtcgcg ccaagcggag gaaagaaaga tcactttgac ttgcggctga atcagacaca
     4801 ggtgacgcgc gaaatcgatg ccatctccgt cggggagttg cccatccaaa atggccagat
     4861 cgaaggtcgc ttgggtcagg cgttcgagcg cgtcggcaat cgtggccgat tcgagtactc
     4921 ggtggccctc cgccgcaacc acggccgcca agacctcccg catctcatcg tcatcctcga
     4981 ggagcagaat ctcggccatg cgtctaaatg ctgatacgag cacacaagcg cagacttaag
     5041 tggctccgag cgcagcagcg aagggccaga acacgaagct cgcatacgtc agctcccgcc
     5101 aaatctctga cgcctgtatc gcgctcgcgg agcgcaccgc tgccgttaga ttctgcagaa
     5161 agcgtaagtg ctccatgccg gtcgcagtcg ggcgcccgtg cgagcaacga cccgcgccgg
     5221 ctgattcccc ccaaagcgca tgaatcactc caaacatgac gagcctcaga aacaagtaaa
     5281 tatgtcagct tgggagcggc gccggcggag tggatccgtt aagtggggag caggcacatc
     5341 cgtttcactc ggagaggtac ccgtctccag gatcgagcgt tgaaggatgg ctcccgattc
     5401 cctcacctcc ggcgaagctg cacctgacga ggccgcgcgg ctgcgtgcgg ttcatcagct
     5461 cgagttgctc gacactcccc cggaggaacg cttcgatcgc atcacgaggc tcgccgcgca
     5521 aaccctgcac accccgatcg cgctcgtgac gctggtagat cgagagcgcg tttgggtgaa
     5581 gtcgcggcat ggttcgcgaa tccgtgagat tgcacgattg ggttcctttt gcagcgaaac
     5641 gctggcgcac gacgaagaat tcagtgtacc ggacgccctg gcggacccac ggttctgcga
     5701 gcacgagcta gtccgaagcg aacctcacgt ccgcttctac gcggggcgaa gcctgcggtc
     5761 gagcaacggc tttcgcgtcg gcacgctctg tattctcgat cgagttccgc ggcgcctcac
     5821 gccggatcaa gggcgagccc tcgcagatct agccttcttg atcgaacgtg agctcgcgtt
     5881 gggtgaccgc atgcggctga agaacgaatt cgtggccacg gtaagccacg aattgcgaac
     5941 gccacttact tctattcgtg gctcgctcgg gcttctgctg ggggaagcgc tcgggcggct
     6001 ccccgagccc gtacagtcga tgctgcaaat cgcgcactcc aatagcgaac gcttgatgcg
     6061 catcgtgaac gaccttctgg atatcgacaa gatcgaggcc ggcaagttgg acttcgtact
     6121 cagccgcgcg agcctgcagc ccatcctcga gcgctcgctg atcgagaatg gtgaactggc
     6181 gcgcgcgcgc ggagtggagt tccggttgca catgggcgcg gcagatgcct gcgcacgcat
     6241 cgatcccgat cgtttgctgc aggtgctcac gaatctgctc tccaacgccg tcaagttctc
     6301 gcctccgggc gccgcggtcg agctcgaact ggagccgcga tccgagcaag tgcggatcgc
     6361 ggtgaaggac catggccccg gtatccccca tgaatttcgg gcgcgaatgt tcggtaagtt
     6421 cgcgcaggcg gacgcctccg atgcccgcag ccggggtggc tcggggttgg gcttgaacat
     6481 cgcccgcgcg ttgaccgagc gtatgggcgg gcagatcggg ttcacatccg agctcggtct
     6541 gggcagcacg ttcttcgtgg atctacctcg ctgcggcgct ctggatcacg aacactccat
     6601 atttttgccc cgcgtcctcg tgtgcgaggc tgatctcgat acggctcgga cgctggccat
     6661 tgatctagaa gcccacggct tcatcgccga tttggtaagt gacccgagcg ccgttcagcg
     6721 cgcacttcgc gatcgctctt actccgccct gacgttggat ctgcgtctac ccgaatcctc
     6781 ggaggtactg cgaacgctac gtgacgaccc gcgcacccga gatctagccg tggtcgtcgt
     6841 ctctgcggat gtgaccgatg ccgcatccca gcgcgccgcg gtcggcctcg aaatcgcggg
     6901 ctggctcgaa aaacctttcg gccccggcga gctcgtacgc cagctgcggg ccgcgctcgc
     6961 gcgtagagag cacggcgccc cgcgccttac ggcgtgaact catggcccag gaactaaaac
     7021 gcattctgat cgtagaagac gacgaggaca tccgcgtcat cgcacgcatc gcgctggagg
     7081 cggtgggcgg tttcgaagtc gaaagctgtg aaaccggcgc caaggccgcg ctcctggccc
     7141 gcaccttccg accccaccta gtcgtgctcg acgtgacgat gcccgacatg gacggcccca
     7201 ctacgctgcg cgccatgcgc aaagaacccg gcctggagct cactcccgtg atcttcctca
     7261 cggccaagca tcacccgcaa gaactccgag aactcgagca gctcggagcg ctcggcgtga
     7321 tccagaagcc gttcgacgcc atgcgcctgc cgagccacat tcagcagctg tggaagcgcc
     7381 gctttcacac cgagtgacgc tcagcgatcc cgctcgatgc ggcaggtctt cacccgcgcg
     7441 gccgaaattt gcccgctctt tttgaacggc tggtccgggc cgaagcgcac ttcgacgtca
     7501 tccactgcgc ccaggcgttc acccgcgcag atcactctaa ctcgctctcg cgggtgcgac
     7561 ttggcctgtt cgtaccaagc cgcaaccgca gggtcctcga acacgcccgc gagcaacacc
     7621 agcttgcccc cgagcgtaac gtaaggccgt aggtttccct caccgacacc acagcgtgcg
     7681 cccacgaccg gtcgtgggct gcggcactgc aggcctcgcg catcgggtgg caccacggtg
     7741 atggcgatct ccattttggt cgagcgacga acggcgaagc tgggccaagc gtaaatcagt
     7801 gccgcggcca acaacgcgcc gagggcaatg cgtagctcat tgcgatcgaa tgaccgaatc
     7861 atcgccagct ccccaagaac aagcgcgccc tcagctgaga aggggcaagc tctgcaaacg
     7921 accagaccgg cgcgtaaaac gcggcgacga gcaacaacgc gaccaagaac cacaacaccg
     7981 ctgcgcggtg ttttacctcg gcatgcgcca gaaatcccgc caggatcagc agtagagccg
     8041 cgtatgcagg caagaagtga tagatgtaag agtctcgatg ggtcagcacc cacggcgcga
     8101 cgaagcccac ggctagggcc aaagtgcgca gtaacccgcg actccgctgg aacacgaaac
     8161 tggagagctc gagagcgagc ggttggtcgg caagggcacc cggcaatgaa gcccggcgcg
     8221 cctcccccga gtcgctcagc accgcccgca aaccaaacca agcaatcaca ccgatcacgc
     8281 ccaggctcag cgccacactc gaccaccagg ctactagatt gcccagtgag ctgagcgccc
     8341 gcacgctacc catatcatcg ccgtatccca gcatcagcgg ccgcacgggt aaccaccacg
     8401 tactccagcc cgaagtggca gggttcttca tgtccgtcag ctcggagtga tgcaggtaga
     8461 gccgcttggt ctcgaaccag acgaagctca aggtcgcggg ctgcctcgcg gtgtgcaaac
     8521 cgaacgcgta aatcccacaa tacaggctga cggccgtaac cagcacgagc gcgatcagcg
     8581 ccgcccgctt cgaccatgtt cccttcgtga acgcgagcca caccaagata ggcagaagca
     8641 aagctacgcc actgaacttg atggccagcg ccaagccgag cagggccccc gccgccaacg
     8701 ccgtcacggg cgacgcggcg aagctcacta cacagagcag gcccaagccc acgcacatta
     8761 gatagccgtc gagcagggcg gcccgcgagt acgcaatcaa gaacccgtct gccgcgcaca
     8821 gacccagcgc gatccaacca gcgcgcggac tcttgaacga gcgcgcggcc gcgaacgccg
     8881 cgaggcccaa gctcgcagct ccgagcaaca aggccggcgc gcgccacgcc gcgctgttgt
     8941 ccccaaacaa ggcaatggac gcggcaatca acagcttccc gagcggtggg tgatcgtttt
     9001 ggtcggcctg gcccgcgagg tagttccgcg cgttgttcac gaagtggtgt tcgtcgaaca
     9061 agaattcgcg gggcaagctg aagtcgcgca gtcggcagac cagccccagc accaccagag
     9121 caaccaataa caagtcgctg gcgcgcattt gcaagccggc gcgagtcgtc cccgtcggtt
     9181 cgacgggctc ttccgggtcc cgcttcaccc gatcggcgcc accccttccc tcgttatcca
     9241 agggcggtcc gtccacaacg cccgcagcca aatcttgccc ggtcatgttc gcgcgcagag
     9301 cgtgtgcgag acggcgagcg cgagcaagtg aaaggctccc cggacggcaa gaagggagca
     9361 gcttcgggcc gatcgctcag cgcggacgca tggaaacgcg cagcatcggc atcgagggca
     9421 cgttcggccg ctcaatcaag ccgtgctggt cgagcaggct ctggaccatg atcaactcgc
     9481 ggccgagcgc ctcggcgtcc tcgtccaact tctccaggtg attctccttg tagcgccgct
     9541 cgatgctctc ggcgaaggag gccatgccca ccgcaccgaa attgccagcc gagcctttga
     9601 tcgcatgcgc caacgacagc accgcgacct tatcgcgctg cttgacggcg acgagcagcg
     9661 cgtgcacgcg cttgccgata tcgccgagga agatcctggt cagctcgagc gcgatgctcg
     9721 cggagcctgc ctcgtcagcc agctcttcga ggcgggaaag atccaggtgt tggttgtcgg
     9781 ccgaagccgg cgcgggcacc gtgagcggag tttctgccgc cacgttgctc gcatcgcggc
     9841 tacgcgcgac ccagtacgct acgcgagaac gcagcgtggc caagctaaat ggcttggtca
     9901 cgtagtcgtt catccccgcg tccagacaag cacgccgtgt accgctcgtg cccccggccg
     9961 ttaccgcgat caccggcatg cggcgcggag cgcgctcgcg catgcggatc tgccgagtgg
    10021 tctcgaaacc atcgaggccg ggcatgtggc aatccatcag caccacgtcg tagtcgtcca
    10081 tcgcgagctt ggacagcgcc tccatgcccg tgctggcctg ctccacccgg cagccaacgc
    10141 cggccagagc gtgcgtgatg agcgcccgat tgaactcgtt gtcttcgacg accagcacgc
    10201 tggcgtccaa gcgcgagtcc gggcgcagcg cgtgatcgcg gctacgcggg acgtccgagg
    10261 ggcgcccgct ctcgcgcggt tcgtcgagca cctcgttcaa aatcgcgagc agctcttgcc
    10321 ccgaacggtg aaccccctgc accgcggcgc gttgatcggc gtcgagctcg gtctcgagca
    10381 acagctcgct cgcgccgatc accaccgtca ttggcgtgcg gagctcgtgg ctgagcgccg
    10441 tcccatcacg cccgaacgag cgcgctcgga tcatgccgcc cagtcgcagc agcagctcct
    10501 gccagcgcgc cgaatcgggc gcagtggttt cgttcagacc caggctttcg agttccgcac
    10561 agagttccgg aggtagcgag aagctacgcg gacgctcact actcatgcaa cgtcctgcga
    10621 cagatccaag gtggcgatgc gccggtacaa ggtggcacgc ccgatcccga gcagcttcgc
    10681 ggccttgccg acactgccct gagtcgcgcg cagcgcgcgc tggatggcgc gacgctcgag
    10741 ctcacgcagc gggatgattt cctcttcccg ctgcgcttcc ggggcggctt taccgttcac
    10801 cggaggcaag ggcggcagca ccgcgttccg gatgtcgacc ggcaaattcg ccagcgcgat
    10861 ctcctcgcct cgacaggcga gcagactgcg atgcaccacg ttctgcagtt cgcgcacgtt
    10921 accgggccac cggtagtgca ttagcgcatc gagcgcctcg ggcgagacgc gatcgatctt
    10981 gcgctccgcg cccacctcga gcgttcgcag gaagtgaccc acgagcaacg gaatgtcttc
    11041 caatcgctcg cgcaacggcg gcaagtggat gggatagacc accaaccgga agtacaggtc
    11101 ctcgcggaag cgccccgctt ccacctcggc cctcaagtcc cggtgcgtcg cacacacgat
    11161 gcgcacatcg atcggcacct cggtcgagcc gcccacgcgt cgcacggtct tctcttgtaa
    11221 ggtgcgcagc aagctcgcct gggtcgtcaa gctcatctca cccagctcgt cgagcagcag
    11281 cgttccgccg tgagcctgct cgaaacagcc gcgccgcagc tgcgtcgcac ccgtgaagga
    11341 gccgcgttcg tgcccgaaca gctcggactc ttgcagcgac tcggggatgc cggcgcagtt
    11401 gatggccacg aacggaccgc ggcgacggtt gctgccattg tgaatggcgc gcgccacgag
    11461 ctccttgccc gtgccgctct cgccgaacac acacaccgct acatcactcg acagcacccg
    11521 ctcgacctgt tgggccagct cgcgcatctt ggggctctgc ccgacgatgg agccgagcac
    11581 accacgctca cccagagcat tctccaggtg acgcacgttg ttcaccagct cgcggcgctc
    11641 tcgcgctcga tgcaccgcca agagcaggcg atcgcggtcg agcggcttgg tcacgtagtc
    11701 gtacgcgcca gctcgcatcg ccgcgacggc tgtctctagc tcgcgctgcg ccgtgactac
    11761 gatgatcggc aggtcggcgt cgcgggcgcg caggtgttga atcaccttga agccgcccac
    11821 ttcacccaac ccgagatcaa cgcaagccac gttcgggctc tctccttcgt cacggatcgc
    11881 gctttcgccc gactcgtgct cgaccactct ataaccgacg cgttccagcc aaaatcgcat
    11941 gagtctgcgc gccgtggggt cgtcatccac gacggctacg agctcaccat tggcgggcgt
    12001 cactggggct tgaggctcct cgtccattcc acttctcccc aacgtccgaa cccagtctcg
    12061 cctttagact ttggagtctc agccagcgac gaaagacgcg cgattagaca tttttccggc
    12121 cccgcagccg ccacccagag ttctccaagc acttgaatag attactcatt catcgacgag
    12181 gctgaacttt tacagcttgg aacgggactc gcagtagccg agcgcgtgac cgcgaaaaag
    12241 cctttcctcc gtgctctgcc gaccgagttt ggcctgttgc gtgtgagcgg cgcgacctgg
    12301 gccgaagctt cggacaagac atccatcaac gtcatgatcg atgatcatgc cgagctgtgc
    12361 gcggtcttac tcgaagttca cccgcagacc gatcgagccg ccgttcccag cgaggggagt
    12421 tcaccggccg cgctgctcgc ggaagtgcag gacctcgtgc tggaggccct gacgcaacgc
    12481 gctccgttgt acgaggtcgt gagcagcacg cgcaaattct gtgcggcgca cgcgggctgt
    12541 gaggtgggca tcgcgctgat gcgactctct cagcgtgact cgcgcgcaga gatcctgaac
    12601 gccggcatgc cggcgatcgt ctggatcgcc ccggacgctg aaatcgtgct ccacccggcg
    12661 ctttccggcc gcatcggcca cacacccggc gaggtgcatg cttacgaatt gtgtccccta
    12721 gtgtggggca gcagctggct catgatgtcg gagtccttcg tgctcgctca accggcgcac
    12781 accacctctc aggaaggcac gccgggcagc gagcggttcg acgcgcagag ttggctcagc
    12841 gaacaagacg tcgcctacga cgcccacctg ctcgcacgcg aaacgcgcga gcggctctgc
    12901 cagcgaatgc actccgtcga tggcccggta cctgccgacg acgcggcctt ggtttgcatc
    12961 gccgccagca gcgaaccccg gttctgaacg cgcctccgcg aagggccgga accacggtga
    13021 aagctcccga ggtcaagctg gttgccgtgg attccgggca gtgtagcctg gcttaccgat
    13081 gagaattcgc accaagacca tcgcgcgctt gcgagacgcc ctgctcgaga gtgggcgccg
    13141 gcccaccctg gtcacctcat ccgcgtacga gacgttggca cgccagggtc tgctctcgcc
    13201 ggaggagctc accgcactag cgcgggtcga gccggtggcc gagttgatgt tcttgatgat
    13261 ggccgcggac gggaacgtcc atgagctcga acgcgacgcg ctgcgcggcg ccatccgggg
    13321 cttggccggg gatacgttac gcagcggaat catccaggtc atgctcgaaa gcttcgagga
    13381 acgcttgcgt aaccagggcc ggctgcagcg tctgcacgag ctcgccgaag agctgagcga
    13441 agagcgcgcc gacgccgaaa gcgccttcac gctcgccgcc gcgatcgcgc tcgcagacga
    13501 ccacgtcgcc gacgccgaga acgagttctt gaatcaagtc gcagagtggc tcggcttgag
    13561 caccgagcgc gcgaaccagg ttttggacca actcgagcaa gacgcagccg aatagccggg
    13621 cggccggggt tcgtggaagg gactaccgtc agcggcggga gcgacgaagg gccggagcga
    13681 tagccacgca gccccgcgcg tccgttgcgc cgagcgagag cccgcccgtc gccgtcgagg
    13741 cgagccgcaa atgatgcagg acctcgctcc gttcggcatc gctcaactcc gcttgcggct
    13801 cccaatcgtc gatccggtat gggcacacgg cgaagctacg ctcgccctct tccgcgatgc
    13861 tgacgcgagc caacagacca tagcgcgtcc aaggatgctc cttgcgtcgc ccgaacagca
    13921 ggtttcccag gctgtagaac accgggcgcc cgtcgaagaa acccacccct tgtaagacgt
    13981 gtgggtgatg gcccactacc aagtccaccc cggtggacat caccgacttc atgaagtttc
    14041 gggtcaagtc gctgggcgca tcctgatact cggcgccccc gtggtagctc acgatcacca
    14101 catcgtaagc ttcacgcgcc gcgcgcaaga tcggctccaa cgctttgaag ctggcccagg
    14161 ccacgcgcgc tctggcttcg tgtcgatcga tgggcccgaa gttccagacg tgcgtcaccg
    14221 cgaacagcgc cacgctccag ccgccaaccc ggcgcacgat gggtgcataa gccgccgcca
    14281 ggtccgggcc ggttccggcg tgatcgacgc ccgcggcatc caagttcacg agcgtctgtt
    14341 cgaacgcacc tggcccgtag tcccacgcat gattgttggc ggtagatacc caatgtacgc
    14401 cggctcgacc gagcgcgcgc gcgccactcg gcgggccagt aaagatcaag cgctgcagcg
    14461 gcgactgcgt ctgcccttgc tggtcactca gcgtcgactc cagattcacg aatctcaaat
    14521 cggcgtcatc ccagaggcgc tcgatgcccg ccaacggcgc gtactccgga tcctggagca
    14581 gacgttgccc cgcctcccgc ccgaagctca cgtcccctcc ggcgacgaac acgatctcgc
    14641 gcgacgccgt gggtatcgac gtcggaggca cttcgctcgg cacgacggaa accgctctca
    14701 ctaacgggcc tcgctccgag agccgcggaa gcggagccgg cactgacgaa agcggccgtt
    14761 ctaaggtggt tgtattcgag ccctccgtaa agactctttc accctcgaat tgccggcttt
    14821 ttgcgggcac cccggtgctc ggtgcgcgca gcttggccat gactgcgacg ggcacggccg
    14881 cgcatgtaac ggccgcacat gtaacggccg cgcacgtaac ggctgcacat gtgatgagca
    14941 gtagattgac tatgccggtg agtacgaccg gaacgccacg cgacgttgcc ggaaaacgct
    15001 cggaaaactg gctcgagttg cgtttcttca cagcgcccag agcctcgggc tcgacagaca
    15061 ccgagcgagt gcggtacttc gggaggtaat gaaaattgcc gtcattggga ccggatacgt
    15121 cgggctggtc gcgggcgcct gcttcgcaga ctctggcacc aacgtcgtgt gtgtggacgt
    15181 cgacgagcag aagctcgaga agctgcgccg aggtgacgtt ccgttcttcg agccgcgctt
    15241 ggccgaactc gtcaagagga actggccgca tcggctggac ttcagctcga acttggccga
    15301 atccatccgc gaccgcgagg tcgtattcgt ggcggtggga acgcccccga acgaggacgg
    15361 ctccgccgat ctctcgcacg tgttgacggt ggccgaacag gtcgcgaagg cggcaaccca
    15421 tgacctcgcg ctcgtattga agagcaccgt accggtcggc accaatgagc gggtcaccga
    15481 ggtcgtcaag cggtacgcga aacatcgtat cagcgtcgtc agcaacccgg agttcctgaa
    15541 agagggtgac gccgtcagcg acttcttcaa gcccgatcgc gtcgtaatcg ggaccgacga
    15601 cgaccacgcc ttcgaagctt tggctcgtct atacgcgccg tacaaccgcc agcgcagccg
    15661 tatccagcgc atgagcccca agagcgcaga gatcgtgaaa tatgcggcga acgcgctgct
    15721 ggccgtgaag atctcgttca tgaacgagat cgccaagctg tgtgacgtag ccggaggcga
    15781 cgtcgagcag gtccgcgtcg cggtcggctc cgacgagcgc attggcatgc aattcctgta
    15841 tcccgggctc ggatttggtg gcagctgctt ccccaaagac ttgcgcgcgc tcgtgcgtac
    15901 gggcgaagag ctgggcgtga agctcgaact cgccgcggcc gccgtgcgtg ccaacgaagt
    15961 gccggtgcgc acgttgctga gccacatgga ggccgacctc ggcggcgtcg cgggcaagac
    16021 catcgcggtc tggggcatcg ccttcaagcc gcgcacggac gacgtccgcg aggcgccctc
    16081 actcaagctg attcaggagc tcgtcgcgcg cggcgcgaag atccgcgcca cggatccgca
    16141 ggccctcgag acgggcgggg atcgcctgcg tcaactcggt attaccgaca acgtacagct
    16201 gttccagaaa gagtacgacg cgagcgaggg cgccgacgcg ctcgtgatcg ccaccgaatg
    16261 gaacgaatac cgaaaccccg acttggggcg actcaaagag ctgatgcgcg gtcgcttcat
    16321 cttcgacggg cgcaacgccc tcacggccga cgccgtggcg gacgcaggct tcgtgtaccg
    16381 cggcatgggc cggccgcgac gcaactgagc tcgcacgcag tccaaacccc atcctcacgc
    16441 ccgcgagctt gcccggtgcg agcctcgcgg gcgtctcggc cttctaccgg ccgcgatcgc
    16501 gctccggcag taaaaccgaa ctggtcgtca gcccaggtcg cgcaaacgat ccagcgcgtc
    16561 ctgcttgaac cgctcgatta aacgcgccac gtactctcgc gcgtcggctc cctcgaccac
    16621 tggcagcagg tccatcgcca acgttgccgc gctcgtcgcg tccgcgctgt acgccgcgtc
    16681 gaacgtcgcg ttggcctggc ggcgcccctg cgtcgccaaa tcgtaacgtt tgttggccgc
    16741 aatctgcgaa tcgaagacgc ccagcagcgc ctgctgcaca tggctacgcg agctgagcgg
    16801 caacaccttg cgatccgctc cactgagcca gtccaagtcc cgccaaacct cacagcccca
    16861 aacctgcgtc ggacgccgct ctggttctag cctcttgcaa gcttcgatcg cggccagcgc
    16921 cacgaccacg tgagtggcgt gcgcgtccgc cagattgtgg gtgtagacca cacgcggccg
    16981 cgtaatccgt aaaatctgct ccaaatccgc gaccagcgcg cggcgcgctc ccgagccccc
    17041 gtcgcgcaaa ctcgcgctct ggaaatcgag ctgcagctga gcggcgtact cacccacgta
    17101 agcggcctta cgctgctcgc gtcggcgcac ctcccgaagc gctgcatcac tgagctcagc
    17161 gtacggcccc ccgcgcgaac taccgcgccc gtccgtcacc accacgcccg caaaccactc
    17221 cgtctcgctc tggaaaccag ccaaaatgcc gtgcgcggcg aagatctcca aatcatccgc
    17281 gtgcgcgcca atccctaggt atgtggtccg agtgagcgcc gcgtccaacg ccgcgccatc
    17341 cggcacgaac acatccgcat ccggattcga cagcttcaac cgattcgagg agggcgattc
    17401 gaggctagcc atgggttcga agtccacgag acgcggcacg atgtcacgtt ccacgcagtg
    17461 taacacccca tttcaaatga cgatccgaat tgggctactc cccaaggctc gcaagcaatt
    17521 ggtttggccg ccatgtaaac gggcgcgacc acccgtcgtt cgcgcccccg agcgcagccg
    17581 cgatcaccca actccgcact cgacaccgcg caggcccgcc tcaatcacga gaccccgatc
    17641 cgccgaagat caaccgcacg agacccacct gcttcactcg acgcctccaa accgattcag
    17701 caggacgatc acactcttcg gcgtaacacg cattcgactc agagcccgtt cgccacgagc
    17761 aatctactca tctcgaaccg ctcacccgat tccattacat acgatgggcg ctcgactcca
    17821 taacgccatt gggtgcgtaa tcgagtctgc atgataagct gacgcatgat gcataggcac
    17881 cttcgttggg gacgaagtct agccgcgttt gctggcatgt atatgctcgg agcgtgtacg
    17941 gtgtcggaag gagggcgggt ttatcacggt gctagcggcg gcacgtcgca gtccagtcaa
    18001 ggtggtgcgg gaggagctcc cagaacgtct tcgctgggtg gtcgaatgaa ctccacgacg
    18061 ctcgcgacgg ccgggggtga acaagcaggc gacgcaaacc ctggacaggg aggagacgcg
    18121 ccggcaggct cagttgctgg cgaatccgga gaacggggca cttcgccggc tggtgcggga
    18181 ggaacctccc catcgcaggc aacgaacgac ggcggcacgc tcagcgacgg cggcacgctc
    18241 agcgacggcg gcgcggctgg atcgcagagc acggcgcagt gcacgcccaa tcgcgcacgc
    18301 tgcacggcga atgtactgca aacatgcaac tccgacggcg cctggagcga accggaggag
    18361 tgcgccgcgg ctactcccgt ctgtttcgag ggagcgtgtc atgcctgcgc ggaaggtcag
    18421 aaacgatgtt cagagaatct ggttcagacc tgcaacgcat ccgggaattg ggagactacc
    18481 cagagctgca cgctgggctg cacagcaggc aagtgtctac tgctgtagta cgactaacga
    18541 gtgggggttt ccgcgaacgg atcaccgagt gacggatcac tcaaaaacga ctcgtccgtc
    18601 aacgactcga gaaaagccaa caagtcgtcg atttctcgcg cttcaatcgt aaagcccgat
    18661 acgaacgcgg acttcaaagg cgagcgagca ccgtctccgg cgtcggggcc ctctgcaatc
    18721 agccgcccac cccttgcgta atgtaactcg agcacctccc gtagagttgc gatactcccg
    18781 tcatgcatgt aaggcgcggt gacggcgaca ttgcggagtg taggcggcct gaaacgaccc
    18841 atatcctcgg ctttgcccgt aacctcgtat acgccttgat cgcgcgctgg ataggcccca
    18901 acgccatcga cgttgtacaa ggcgtcattc tggtagaaca gctggtcacc gaccttgccc
    18961 tcccaggtcg cggcgttgct gaatgcaaac gctccgtggc agtggtgaca ttcgaagcgc
    19021 tctccaaaaa acaacttggc gccgcgttgt gcggactcgg atatagcgtc accctcttcc
    19081 cccgcagcat accggtcata gggtgaattg ccggaaatca atcgtcgctc gaaggaggcg
    19141 atggcacgca cgatgttgta gagattcagc gggtcctgct cgtctggaaa cgccgctcgg
    19201 aacagctctg gataacgggc atcgctcttc aggcgatcta cgagctcgaa ctctcgcccc
    19261 gagaagccca gctcgaccgg gtcatctccc aacagcggta cagccacctg catctcgagg
    19321 gtcttcagca ccggattggc ccaggtttgc gctgaattgt aggcggcatt ggtcaagctc
    19381 atcgagctgc gccggttcac tacaccagtg gcgcccaccg cgttcgcgcg gccgtcactg
    19441 aaggccaacc tctgttcgtg acacgacgcg caggactggg tctcgttcga agacagccgc
    19501 ttatcgtaaa agagccttcg accgagctca gccttttcag cagacatcgg gttttcctgc
    19561 ggcaccttgg gtttgggaaa tcctgcgggc agcttccatt cgtaagcggt gggttcgcca
    19621 gtctgcccgc tgccacagcc tgtggtgcca gtcagcgcgc cggtaagcgc caaccccagc
    19681 accgatccga agtgtccggc ggtagcgatc gtgctactcg gctttgaata gcgcttgctc
    19741 tttggcatcg gattcggtgc tttccgcgat cccgagtcga ggcagcaatt cggagcactc
    19801 agggtcgtcc ggctccgaca tgcagcctgg agcggtgccc tccgtgttga acgacaggtc
    19861 gctggtcgca aatagctcgc cgagatctag cccgatcttc ccgcttcgca ggggcaatcg
    19921 atagctcgcc cgattgggcc gctcgcaagc tccggtgggt gggcgctgtg gtttgtctcc
    19981 ggggcagccg ctggcgccca cgtgcaggat gaaagagtgg atcgcaccgt ccagggatga
    20041 cgccgcccca tccaccttga ggaacttgta tccatactgc caaatccaga acatcccggt
    20101 cacgtcgaga ggcggagccg cgctggccaa gtccacgaag ttcttgcgat ccgggacgcc
    20161 caacgaaaat cgcacttcac tccaagcgtc tgcgctgccg gtcgaaggca ccaccacccg
    20221 tccggagacc gtagtgtgcg tctcgggggt accatccgat ccgcagccgt cctcgaaatc
    20281 caagagggcc agaccgtcgg attgaaaagc gttctcttcg agctccactg gcgaggtctc
    20341 gccggagccg tccatcaact cgaagttatg aagatataga cgcgcgtccg aaatgctgaa
    20401 ctcggtcggc ggtgagccca cggacggata gcgcgcacca cactcgaagg gcgccccatt
    20461 gactcgcaaa tcaaactgaa tctccacgtt tcgatatatg gaggagtcct tcgattcgtc
    20521 atcaccgcag ccgaccaaag ccgccgcggc gaaccccagc aacgcgtagc gcgccactaa
    20581 cgagacgtgg atggtggaaa tagatcgcac ggtaatcctt tactcggcgg ccggggcaac
    20641 tcgccggggc gttcgagaca gggcatgggg attgatgagc ttccggcgcc agcgctgccc
    20701 cccgaaccgg ggctgcctcc ctgcaaaacg ccagaagccc ttggctcgga atcattacta
    20761 catccggtag aactggtgcc caacagcagg atcaggagac cagcggggct gtgccggaac
    20821 atttcagtac ttcacccaaa tctcgtcgcc gctcgccagc gcaccgagag tggacacgcc
    20881 actggtttcc gcaatcagct cggtcgtcgt gggattcgag ctatacgcgg agtttccgcg
    20941 ccaaacgatg actgcatcgg cgaagtaagc cgacaaatcc tcgattgccg aggagacctg
    21001 gaatttgttc cacccggggt agcgcttcgg caggatcttc agcgcgggag ccggctctgg
    21061 ccctgactcg ctctcgctcg gttcagtcga tgcccagtcg acgatcagct cttccccctc
    21121 ggcgctcagg caggtctgcc gagtggggaa atagatcgtg ctgaaggggc gattcgggac
    21181 cttcacgcgc aagctcacct tgtaataatt ggaatctgaa ctgagcacgc tcagggccgg
    21241 cttctgccag ctgacgaacc gaacgagacc cgcgtcgtct agctcaatcg tggccgggcc
    21301 cagcgtcgcg tccaccgcac ggaccgacac cacttcggcg ggaatttcga tgcggatccc
    21361 ttgcgtatcg ctgccatcgc agccgtggcc aacgccgaac acgatctctt gattggtgtc
    21421 cgcaaaacca ggaccacta
//
LOCUS       DASTKU010000193         7587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_706162, whole genome shotgun sequence.
ACCESSION   DASTKU010000193 DASTKU010000000
VERSION     DASTKU010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706162"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VFQ61_07370"
     CDS             <1..793
                     /locus_tag="VFQ61_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9954305.1"
                     /translation="EKPLAPSLAECQAIQNAAQKAQRYLMVAYRLHFDEATLKAIDIA
                     QSGKLGELRLFSSFFSHVVRPGDIRTRDELAGGALLDLGVYCVNAARNLFRAEPISVS
                     AQIIERDGADDTTSATLGFPGDRIAQFVVSNSTAAVSSYRIAGTEGSLRVEPGYEYRE
                     PIEFFLTVGDSNDHYKFDVGDQFGPELVYFSNCILQSREPEPSAEEGILDIRVIEAIF
                     QAARTGTTVKLEPRTRGRRPNTLQIMHKPAVGKQETVHSESPSIK"
     gene            complement(947..1471)
                     /locus_tag="VFQ61_07375"
     CDS             complement(947..1471)
                     /locus_tag="VFQ61_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HET9954306.1"
                     /translation="MSELRAPKNNLSSGSELGFGIVTRYARPSATFRRGIQFVLSCCC
                     SLSSVGCGSSLPTPPSTQHPRGAFTAVPYPPPAALAETLVPRPERADVVWIDGGWIFR
                     GRSYAWQRGGWVVPPEGGRFALSKVVFARDGRILYAPSAWYDQEGTQLDRIRPFAPAA
                     TPPNEYTAETQTSR"
     gene            1610..3751
                     /locus_tag="VFQ61_07380"
     CDS             1610..3751
                     /locus_tag="VFQ61_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9954307.1"
                     /translation="MRNSLLLSLMIALASIACAPLPPGRYTLDKVQVTGNSAVDDDDI
                     ESVIASQPSARFLGVLPGFIYDYSVFDRFVLERDLQRIERYYHAQGYYHARVRAARVT
                     YKGRGARVLIEVEEGPATVVQRVDVHGLDELPEPVRRRANAAVSSELPLGAPLKEESF
                     ENAAKALKDQIEDEGYAYVDLKRSAEVDLPRNRASIGFWIKPGPKAEFGNVRIEGLGP
                     LPEPPVRRALDLQPGQPYSRFDLDEAKRALLDLNVFSTVSIEPELTEEQLAKKPRRVP
                     ILVRVEPSKLRSVHAGVGVQIDTQSANVHLIAGWEHNNFFGGFRRFLVEARPGLVFYP
                     TRLPDVRAPEHVFPALRLRNEFRQPGFLEARTNLLLRAQMNIDPLLLSTKYNPDDKVI
                     GYQDVRASAGLERSFWKLYGVLSHNVQLNYPFSYLGDLDPDLKTVWVSYPELLVSLDL
                     RDDRVSPHRGAYVSLDTQVAGVGGQARDLKIQPEARAYVPLARKWTFASRATVGFLFP
                     QNYGGTVEHNSRRGVPHGRADPALEPGERANWIEDIELMFMRGFFSGGSGSNRGYAAR
                     EIGPHGTVPFYNPGQSVRDTETGMDGAPYCPEKSGVPVKRGCNLPLGGFTLWEASVEV
                     RYPISGPLSGTVFSDASDVSPNKASFRFNRPHLTVGLGFRYATPIGPVRLDAGYRVPG
                     AQAPSTAVDEVEPSKIFGVPVAATFGIGEAF"
     gene            3751..>7587
                     /locus_tag="VFQ61_07385"
     CDS             3751..>7587
                     /locus_tag="VFQ61_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954308.1"
                     /translation="MKSTQPLSRSKPPSRTRRWLRILARIPLYLVGGIVVLLLLVLAS
                     LNVPAVRGFVATRVNSALETTFKGQIVLERIGILNPIGIGGLDVQVLDAAQKPVLFAR
                     GLSLRANWPSAVWNAVRGKPIHLELSPIGCDHIEVIVRDDGTGSPTLAQAFAPRNPSP
                     PSNEPSTFSLSIPKLALQHVWAHGALSSLPLIDTELQDALVSLNLDPNTFALELQRVQ
                     LQARGLPPRANTTGRLAAKLRLPKDPKQLSAQARFEGLLGGGHLQLRAALDRQNVDAH
                     VHATRIPAEFLRSQLPTLNVSGESELELKAQGAMERIALQLRAENPAAKLLLDGELGL
                     LTTPKSAQNVRATLDVSGVNLAGLLGNPNADPNHSGPAGSSSMPKTELGLHAELTASH
                     DETGSMQGTYDLRVPSGKYGAEITPPISARGRLHGTATEPLTIESKILVQEPGAPTEL
                     EIQFVLDEAQRLQAELTSTLNHPSRAKRWGVDTTGTISAKVDAKLATQTFNARLSSRL
                     NGPRQAALRAERVELQGRASGSFSDPQIDLGVDVSKFATGDIRLRSVKAHAAGSLKEL
                     DVSANLVRESGQRVSLRSSVSLAEAITLRDTNLAINAKEGPIQVRVRNASFVGNRISV
                     RNFELTGAGRASGNVMLEGPRVNAQLELQELDAKRLRAASELALPLEAGLFSGRVDVS
                     GSVAALKGELQIQARRVDFGALRDGAAELSLNVKNQVASGHLSAAVGESRFVANIEQV
                     DIPRSFASRQQLMNARGTIDAQGTIVLQTVLPLLQAAGVPVERARGKVELTLSANNPR
                     GGESNPALKFAVATHGLHLVEQRPRLKRIESPSEARAVEPRSLTGIDLVVNGELDARN
                     HKADVSATIRDRRGLLADIQAQTKGLDIFGNTVRAVDHVPMSVAVHIPERRIDQLPEL
                     IRPDAIQGVLRLDVDAQGSLAAPEIRAAVSVDRLRSRARGRAVNLKAELQSTTSGGEL
                     IIVGDTRRNGQVLSLNTKWTGSVIARAMSPTQPTARAELAESRNMLAQSAAPQARTVP
                     PLDLDAELELKEFPLVTLAPLADRQIRGPLSGRVQLKDFGKAAKLDASFDASGLRVGN
                     SRLPAFGVTAKADGKNLTARVQAKQTEGHLSADFETPMTWGAALAPAIEPKARARVEA
                     KSFDLETVGPIVAQYVSALEGTLDADLAVDLTEAKPKLEGRAAVRRGVFQLPQVGQRF
                     SNVAANVELGAGQVKLSHFEARGLSGRLTASAEAKLNGAELTAAKARLDITEREKLPI
                     TLEGVAFGEAFGHADFS"
BASE COUNT         1452 a         2442 c         2338 g         1355 t
ORIGIN      
        1 cgagaagcca ctcgcgccga gcctcgcaga gtgccaagcg attcagaacg cggcgcagaa
       61 ggcccagcgt tatttgatgg tggcttatcg cctccatttc gacgaggcca ccctcaaggc
      121 catcgacatc gcgcagtccg gaaaactcgg cgagctacgc ttgttcagct cgttcttcag
      181 tcacgtagtg cggcccggtg acatccgtac tcgagacgag ctagccggtg gggccctgct
      241 cgatctaggt gtgtattgcg tcaatgcggc ccgcaacctg ttccgagccg agcccatctc
      301 ggttagcgcg cagatcatcg aaagagatgg agcggacgac accacgagcg ctacccttgg
      361 attcccgggg gacagaatcg cgcagtttgt cgtcagcaac agtaccgccg ccgtatcgag
      421 ctatcgtatc gccggaacag aggggagctt gcgcgtcgag cctgggtacg aataccggga
      481 accgatcgag ttcttcctca ccgtcgggga ctcgaacgac cactacaagt tcgacgtcgg
      541 ggatcagttt ggccccgaac tcgtctactt ctccaattgc attcttcagt cacgtgagcc
      601 ggagccttcc gcggaagaag gcattctgga cattcgtgtc atcgaagcca tctttcaagc
      661 ggcgcgcacg ggcacgaccg tcaagctcga gccgcgcacg cgcggccgac ggccgaacac
      721 gctgcaaatc atgcacaagc ctgcggtcgg gaaacaagag accgttcatt ccgaatcgcc
      781 cagcatcaaa tagcgctcat ttgccgttcc gagccaacga cactccgagt acgagccgtc
      841 cgcgaccgct gcactgtaca tcgagcggag tcatcagagg ctgcatctgc gccccgagtg
      901 aggcgtgcag gcggcctcta ttcttccgtc tcgcactggg cggcgctcaa cgcgaggtct
      961 gagtttcagc cgtgtattcg ttgggtggag tcgcggccgg ggcgaacgga cgaatgcggt
     1021 cgagttgagt tccctcctga tcataccaag ccgaaggcgc ataaagaatt cggccgtcgc
     1081 gcgcgaagac gaccttagac aaggcgaagc ggccaccttc tggtggaacc acccaccccc
     1141 cgcgttgcca agcgtaacta cgccctcgaa atatccaacc gccgtcgatc cagacgacgt
     1201 cagctcgctc cggccgcggc acgagtgttt cggccaacgc ggctggtggc gggtaaggca
     1261 ccgcagtgaa cgcgccacgc gggtgttgcg tgcttggtgg tgttggcaaa gaggaaccac
     1321 aacccacgct gctcaatgag cagcaacaac tcagcacgaa ctgaatccct ctcctgaagg
     1381 ttgcgctcgg gcgggcgtac ctcgttacaa ttccaaaacc gagctcgctc cctgaagaca
     1441 agttgttttt cggggcacgt agttccgaca tcgaaggctg tgatctcatg gcgccgccgc
     1501 agcaaacttg gaacaccgct tgcagctagc atgccgtttg atgtgccgct gtacccaccg
     1561 tgccgagtta cggcgggccg gaacacgacg tcatccgtgc atttcgttca tgcgaaattc
     1621 ccttctgctt tcgctaatga tcgcgttggc ctcgatcgct tgcgcgcctt tacccccagg
     1681 acgctacacc ttggacaagg tccaggtaac cggcaacagc gcggtcgatg acgatgacat
     1741 cgagagcgtc atcgcgagtc agccgagcgc acgattcttg ggcgttctgc cgggcttcat
     1801 ctatgactac agcgtcttcg atcgcttcgt actcgagcgc gatctgcagc gcatcgagcg
     1861 atattatcac gcccaaggct attaccacgc gcgtgtgcgt gccgcgcgcg tcacctacaa
     1921 gggtcgtggg gcgcgggtct tgattgaagt cgaggagggc cccgcaaccg tcgtccagcg
     1981 cgtcgatgtg cacggcttgg atgagcttcc cgagccggtg cgccgccgag ccaacgcggc
     2041 cgtctcgtcc gaactgccgc tcggtgcacc attgaaggaa gagtctttcg agaacgcggc
     2101 caaagccctc aaagatcaga tcgaagacga gggatacgcg tacgtggatc tcaaacggtc
     2161 cgccgaagtg gacctgccgc gcaatcgcgc cagcatcgga ttctggatca agcccgggcc
     2221 caaggcggag ttcgggaacg tgcggataga aggtttgggg ccactgccgg agccaccggt
     2281 tcggcgtgcc ctcgacctgc aacccgggca gccctattcg cgtttcgatc tggatgaggc
     2341 caagcgcgcg ctcctcgatc tgaacgtctt cagcacggtg agcatcgaac cggagctcac
     2401 cgaagaacaa ctagcgaaga aaccgcggcg cgttccgatc ctggtgcgcg tggagccctc
     2461 caaactccgc agtgtccacg ccggggtcgg tgtgcaaatc gacacgcaat ccgccaacgt
     2521 gcacttgatt gcaggctggg agcacaacaa tttttttggt ggcttccggc gattcttggt
     2581 cgaggcccgc ccggggctcg tgttttatcc cacccgcttg ccagacgtgc gcgcgcccga
     2641 gcacgtgttc ccagcactgc gtctgcgcaa tgagttccga cagcccgggt tcctcgaggc
     2701 ccgcacgaac ttgttgttgc gcgctcaaat gaacatcgat cccctgctcc tgagtaccaa
     2761 gtacaacccc gacgacaaag tgattggcta tcaggacgtg cgtgcttcgg cagggctcga
     2821 acgctcgttc tggaagctgt acggtgtgct ctcgcacaac gttcaattga actatccgtt
     2881 ttcgtatctg ggtgatttgg acccggacct caagaccgtg tgggtgtctt acccagagct
     2941 gctcgtcagc ctcgatttgc gcgacgatcg ggtgagcccg caccgcggtg cgtatgtctc
     3001 actggacaca caagtcgccg gagtgggcgg gcaagcgcgc gatctcaaga ttcagcccga
     3061 agcgcgcgcc tatgtcccgc tggctcgtaa atggacgttt gcctcgcgcg cgacggtggg
     3121 gttcttgttt cctcagaact atggcgggac cgtcgagcac aattcgagac gcggagtgcc
     3181 acacggcagg gccgacccgg cattggaacc cggggagcgc gcgaactgga tcgaagacat
     3241 cgagttgatg ttcatgcgcg gcttcttttc cggtggttcc gggtcgaatc gaggttatgc
     3301 agcgcgcgag atcggcccgc acggaaccgt gcctttctac aaccccggac aaagtgttcg
     3361 tgataccgaa acgggaatgg atggtgcccc atactgtcct gagaagtcgg gggttccggt
     3421 caaacggggc tgcaatctgc cgctcggtgg cttcacactt tgggaggcgt cggtcgaagt
     3481 gcgttatccc atctccggac cactctccgg aacggtgttc agcgacgcga gcgacgtgag
     3541 ccccaacaag gcaagcttcc gtttcaatcg cccacacctg acggtggggc tcggcttccg
     3601 ctacgccaca ccgatcggcc ccgtgcggct cgatgccggg taccgcgtcc ccggtgctca
     3661 agcgccctcc accgcggtgg acgaagtgga gccatccaag atcttcggag ttccagtggc
     3721 cgcgaccttc ggaatcgggg aggcattcta atgaaatcca cccaaccgct ctctcgaagc
     3781 aaaccgccct ctcgaactcg gcgttggctg cgcatcctcg cgcgcatccc cttgtatctc
     3841 gtgggtggca tcgtggttct gctgctgctc gtgctggcta gtctcaacgt gccggccgtg
     3901 cgtgggttcg tggccacgcg agtcaactct gcgctcgaaa ccacgttcaa gggtcagatc
     3961 gtgctggaac gtatcggcat cttgaacccg attgggatcg gcggcctgga cgtgcaggtg
     4021 ctcgacgcgg cccagaagcc cgtgctgttc gcgcgtggcc tcagcttacg cgccaattgg
     4081 ccgagcgccg tttggaacgc agttcgaggc aaacccattc acttggagtt gagccccatt
     4141 ggttgcgacc acatcgaggt catcgtgcgc gatgacggca cgggctcgcc cacgctcgcg
     4201 caagctttcg ccccgcggaa cccatccccg ccgtcgaacg agcccagcac cttctcgctc
     4261 tccatcccca agctcgccct gcaacatgtc tgggcgcacg gcgcattgag cagtttgcct
     4321 ctgatcgata ccgagctcca agacgcactg gtctcgttga acctagaccc aaacaccttc
     4381 gccctagagc ttcagcgcgt tcagctccag gcgcgcgggc tgccccctcg cgccaacacg
     4441 acgggtcggc tcgcggcaaa gctgcggctc cccaaagatc ccaaacagct ctccgcgcaa
     4501 gcacgcttcg aaggtctgct cggtgggggg cacttgcagc tgcgcgcggc gctcgatcgc
     4561 cagaacgtgg acgcgcacgt tcacgcgacc cgcattcctg ccgagttttt gcgcagccaa
     4621 ctgccaacct tgaacgtctc gggtgagagc gagctcgagc tgaaggcaca gggcgcgatg
     4681 gaacgaattg cgctccagct tcgggctgaa aacccagccg ccaagctttt gctcgatggg
     4741 gagttgggcc tactcacgac gccgaagtcg gcgcaaaatg taagagcgac gctcgacgtc
     4801 tcgggcgtga acctggccgg cctgctcggg aatcccaatg ccgatccgaa tcacagtggc
     4861 ccggccggct cgagtagcat gcccaagacc gagctcgggc tgcacgccga gctgacggct
     4921 tcgcacgacg agaccggttc gatgcagggc acctacgacc tacgtgtgcc ttcgggcaag
     4981 tacggagcgg aaatcacgcc gcccatctcg gcgcgaggcc gcctgcacgg aaccgcgacg
     5041 gagccactca cgatagagtc caagatcctg gtgcaggagc ccggcgcgcc caccgaactc
     5101 gaaatccaat tcgtgttgga tgaagcgcag cggctgcagg ccgagctgac ttcgactttg
     5161 aaccacccct cgcgcgccaa acgctggggc gtcgatacca cgggcacgat ttcggccaag
     5221 gtagacgcca agctggcgac ccaaactttc aacgcgcgtc tatcctcgcg cttgaacggc
     5281 ccgcggcaag ccgcgctgcg cgccgagcgc gtcgaactgc aaggccgtgc ctcgggaagt
     5341 ttttcggacc cgcagatcga tctcggcgtc gacgtgagca agttcgcgac cggcgacatc
     5401 cggctccgca gcgtcaaggc tcatgcggcc ggctcgctca aagagctgga cgtgtccgcc
     5461 aacctggtga gggagagtgg gcaacgcgtt agcctaagaa gctcggtctc gctcgcggag
     5521 gcgatcacat tgcgtgacac caacctggcc atcaacgcca aggagggacc aatccaggtg
     5581 cgtgtccgca acgcgagctt cgtcggcaat cgcatttcgg tgcgcaactt cgagctcacc
     5641 ggcgccggcc gcgcctccgg caacgtcatg ctcgaagggc cgcgggtcaa cgcccaactc
     5701 gagctgcagg agctggatgc caagcggctg cgcgccgcgt ccgagctcgc tttgccgctc
     5761 gaggcggggc tcttttcggg ccgcgtggac gtgagcggtt cggtcgcggc cttgaaaggt
     5821 gagctccaga tccaggctcg ccgcgtcgac ttcggggccc tgcgtgatgg cgccgccgag
     5881 ctatcactca acgtcaagaa ccaggtcgct tccggtcacc tctccgcggc ggtgggcgag
     5941 tctcgattcg tggccaacat cgagcaggtc gatatcccgc gttcgtttgc gagtcgtcag
     6001 caactgatga atgcgcgagg aaccatcgac gcccagggca ccatcgtgct gcaaacggtg
     6061 ttgcccctgc tgcaagcggc cggggtaccg gtggagcgcg cacgcggcaa ggtggagctg
     6121 accttgtcag ccaacaaccc gcgcggcggc gaaagcaacc cggctctcaa gtttgcggtg
     6181 gccacccatg ggctccattt ggtggaacag cgcccacggc tgaagcgcat cgagagcccg
     6241 agcgaagcgc gtgccgtcga gccccgctcg ctcaccggga ttgatctcgt cgtcaatggc
     6301 gagctggacg cccgcaacca caaggccgat gtctccgcga cgatccgcga ccgtcgtggc
     6361 ttgctcgccg acatccaagc gcagaccaag gggctggata tcttcgggaa cacggtccgg
     6421 gcggtggatc acgtgccgat gtcggtggcg gtgcacatcc ccgaacgccg catcgatcag
     6481 ctgcccgagc tgattcgacc tgacgccatt cagggcgtcc tgcgcctaga cgtcgatgcc
     6541 caaggtagct tggcggcacc ggagattcga gcggcggtta gtgtagaccg gctgcgctcg
     6601 cgagcacggg gtcgagccgt aaacctcaaa gccgagctcc agtccaccac cagcggcgga
     6661 gaactcatca tcgttggcga tacgcggcgc aacggtcaag tcctcagctt gaacaccaag
     6721 tggactggca gcgtaatcgc gcgcgctatg agcccgaccc aaccgacggc gcgcgccgag
     6781 ctcgctgaat cgcgaaatat gctcgcgcaa agtgcggcgc cgcaggccag gactgtaccg
     6841 cccctcgact tggatgcgga gctcgagctc aaggagttcc cactcgtcac gctcgcgccg
     6901 ctcgccgatc gtcaaattcg cggcccattg agcgggcgcg tgcaactcaa ggactttggt
     6961 aaggcggcca agctggatgc ctcgttcgat gcctccggtt tgcgcgtcgg gaattcgcgt
     7021 ctgcccgcct tcggggtcac ggcaaaagcc gacggcaaga acctcaccgc gcgcgtgcaa
     7081 gccaagcaaa ccgaaggtca cctgagcgca gacttcgaga cgccgatgac ctggggcgcc
     7141 gcgttggcac ccgcaatcga acccaaggcg cgcgcccggg tggaagcgaa gagcttcgat
     7201 ttggagacgg tcggcccaat cgtggcgcag tacgtgagcg cgctggaagg caccctagac
     7261 gcggatctgg cggtcgacct gacggaagcg aaacccaaac tcgagggtcg ggccgcagta
     7321 cgtcgtggtg tattccagtt gcctcaagtc gggcaacgct tctcgaacgt ggccgccaac
     7381 gtcgagcttg gtgctggcca agtcaagctc agccacttcg aggcgcgggg cctcagcggg
     7441 cgcctcacgg ctagcgccga agcaaagctg aatggcgcag agctgaccgc cgccaaagcc
     7501 aggctcgaca tcaccgaacg cgagaagctg cccattaccc tcgagggtgt cgcgtttggc
     7561 gaagcgttcg ggcacgcgga tttctct
//
LOCUS       DASTKU010000194         5217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_929816, whole genome shotgun sequence.
ACCESSION   DASTKU010000194 DASTKU010000000
VERSION     DASTKU010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929816"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1853
                     /locus_tag="VFQ61_07390"
     CDS             <1..1853
                     /locus_tag="VFQ61_07390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HET9954309.1"
                     /translation="DPRDYGRVLNEWNQGSNAGDSSAYSCAAAELQRWAERAPERTAI
                     VSGSERLSYAELWARVCLLSRGLQGLARPGQRIAVLMGRNVDHVATLFALLHAGFTYV
                     PIDLRWPKERVLAVLGQVRPTWMVTDRSAASEGLELEVPVLDVERLLRGEPGRTFEAP
                     TPPTHPELPAYVLFTSGSTGKPKGIAVSRRNLSAFIEFARGAFSQEELACVAATTTTA
                     FDLSIFELFVPLASGGAVYLCEDLFSLPSEPVVPITLLNTVPSLLEEYLFQRTLPRGL
                     RTVCLAGEPLPAVLVTRLHRSGIRSVWNLYGPTETTTYSTGAEVPRDVKVPSLGRPIR
                     GTRVYVLDEQLRPVEVGVTGELFIGGAGVSLGYCGDPRGTAARYVPDPFGAPGSRLYR
                     TGDFVRWDRAGSLEYVGRRDDQVKVHGVRIELSEIETVLRSLPGIRSAAVTAERSAHE
                     TTLVAYVVSEDAVVLDATRLTRALAERLHPAAVPRRWLQVPALPVNERGKLNRRALAE
                     QQAAAIDARAEVSPPRTETEHALLEIWRELLPESGEIGVHDDFFAAGGNSLLLTRLLY
                     RIREVMGVAIQVADLDHLRSIAALAGHIENIRWLRDGSPSFAGATDRGEL"
     gene            1850..>5217
                     /locus_tag="VFQ61_07395"
     CDS             1850..>5217
                     /locus_tag="VFQ61_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9954310.1"
                     /translation="MRPLSELLAQLRARRVSLRWDGSRLSYEAPAGMLDADLAQELRS
                     RRDELVELLRSDVTSEGSRQQAERSTAPERARCSAQQRSIWAAQSLGLAGPAFHMAFA
                     LHLEGALDPLALRYALDAVLERHQILRTRLIERDGELMQTVDAAAPVELTPTDVSTTA
                     LAALIERPFGLASEHPIRVRLERHGPSRHALYFVVHHIAMDGWSLSILARELSEAYAE
                     RVAGVRRTEQRPALQYWQYAEWQASHLNPARVARAVAFFGEELAGAPAGRPVFRGHER
                     LGPSRYRGAWHPIELEAELTTNLGALATRCRSSLYAVLLAAFSCVLAKHGAGLDWVIG
                     TAVANRTPASVADSLGMFANTLPVRVQLASDPRLVDFVRDCASAVLRSKRYEDVPIDM
                     LVQGLNTEGPSGELGGARSWPFQVLFAYDDSYPSQFELAGLSTRVEPVSTGTAKYDLT
                     LSLARAGSGLTGWLEYDSDQYRATDVARLAQAFRAALNQLLAAPETRYRDLDLLSAQD
                     RALLLREEGHAAPAPPSGTLVELLTRAFGVYAERTAVEQGDETLSYGQLEARSGAIAR
                     ALRKAGVSPGSHVAVFLDRAPSLLCVLVGVLRAGAAYVPLDPRYPEARLRRILERSRA
                     SCLVASPKWWARGMELGARITLDAEQLLHASGEAGELPQAAASQPAYVLYTSGSSGEP
                     KGVELSQGNARSFVDWALAAYAPQELRGVWAGTSLGFDLSVFELFATLCGGGTVLLTE
                     SPIDPPVGKTQPTLINTVPSVLRELLETHAIPGSVVTINSAGEVLPRDLCHALWSRTA
                     IRRLVNLYGPTETTTYVTASEITRAADSIAIGRPLPNASVLLLDEFGERVPPEVTGEI
                     YVAGAAVATGYLHEPRQTAERFVPHAESGAHRMYRTGDYAYRDSSGELHYLGRRDAQL
                     KVRGHRVEAAELECLLRSSPKVRDAVVVLPDGSGPAGSLVAFVVPTAADPNLESELRQ
                     ELAKRLPAALVPGAFFQRDTLPKTSSSKYARRALAREAGLALLERSSATAPLEGYIEQ
                     TLAQIWCRVLGVAQVGPDQNFFELGGHSLSAVRVACLISQELGSAVQGQALFEYPVLR
                     DLSRFMQSRMYSTAGEDQIEALLREVEALSDAEV"
BASE COUNT          821 a         1649 c         1779 g          968 t
ORIGIN      
        1 tcgatccacg agattacgga cgcgtgctga acgagtggaa ccaagggtcg aacgcggggg
       61 actcttccgc ttacagctgc gccgcggccg aactgcaacg ttgggcggaa cgcgcgccag
      121 agcgaaccgc aatcgtttcc ggatccgaac gcctctcgta cgcggaactc tgggctcggg
      181 tttgcctgct cagccgcggc ctccaaggtc ttgcccgtcc agggcagcgc atagccgtgc
      241 tcatgggacg caacgtggat cacgtggcga ccttgtttgc cttgctgcac gcgggcttta
      301 cctatgtacc catagatctg cgctggccca aagagcgagt gctcgccgtg cttggacaag
      361 tgcgcccgac ctggatggtc acggatcgat cggccgcaag cgaggggctc gaactcgaag
      421 tgccggtttt ggatgtcgaa cgtttgctgc gtggagagcc gggacggacg ttcgaagccc
      481 caaccccgcc gactcacccg gagctgccgg cgtatgtgct gttcacgtcg gggagtacgg
      541 gcaagcccaa gggcatcgcg gtctcgcgcc ggaacctttc ggccttcatc gagttcgcgc
      601 gcggcgcgtt ctcgcaggag gaactagcgt gcgtcgcggc aacgaccacc accgctttcg
      661 acctctcgat cttcgaactc ttcgtgccgc tcgccagcgg cggcgccgtg tacctgtgcg
      721 aggatttgtt ctcgctcccg tcggagcccg tggttcccat cacgttgttg aacaccgttc
      781 cctctctgct cgaggagtat ctgtttcaac gcacgctgcc gcgcggattg agaaccgtat
      841 gcctcgccgg tgagcctctg ccggccgttc tcgtcacacg tctgcatcgg tcggggattc
      901 gcagcgtttg gaatctttac gggcccaccg aaacaacgac gtactccacc ggtgcggagg
      961 tgccgaggga cgtcaaggtg ccgtcgctgg ggcgtccaat tcgcggcacg cgagtgtatg
     1021 tgttggacga gcagttgcgc ccggtcgagg tcggcgtgac aggggagtta ttcattggag
     1081 gggctggcgt tagcctcggg tattgtggcg atccgcgcgg caccgcggca cggtatgttc
     1141 ccgatccgtt cggggcgccc ggctcgcggc tctatcgaac cggtgacttc gtccgctggg
     1201 accgcgcggg ctcactcgag tacgtgggtc gccgcgatga tcaagtcaaa gtccatggcg
     1261 tccggatcga gctctccgag atcgaaacgg tgctgcggtc gctgcccggg atccggagtg
     1321 ccgctgtaac ggcggagcgc agcgcccacg aaaccactct ggtcgcctac gtggtgtcgg
     1381 aggatgcggt cgttctcgac gcgacgcggc tcacccgtgc tttggccgaa cgcctgcatc
     1441 cggccgcggt tcctcgacgc tggttgcaag tccccgcatt gccagtcaac gagcggggga
     1501 agttgaaccg acgtgctttg gcggagcagc aagcggcggc catcgatgcg cgcgccgaag
     1561 tgagcccacc tcgcacggag acggagcacg cactgttgga gatttggcgt gagctgctgc
     1621 cggaatccgg agagatcggc gtgcacgacg atttctttgc ggcgggcggg aactcgctgc
     1681 tgctcactcg gctgctctac cgcatccgag aggtgatggg ggtcgcgatt caggtcgccg
     1741 atctggatca cctgcgatcg attgccgcgc tcgccgggca catcgagaac atccgctggc
     1801 tacgcgacgg gtcgccatcg ttcgcggggg ccacggatcg aggtgagctg tgagaccgct
     1861 ttccgagctg ctagcccaat tgagagcacg gcgcgtaagc ttgcgctggg atggctcgcg
     1921 cttgagctac gaggcgccgg ccggcatgct cgatgctgac ctcgctcagg agctccgtag
     1981 ccgacgtgac gagctcgttg agctgctgcg ttcggacgtg acgtcggagg gctcacggca
     2041 gcaagccgag cgctcgacgg ctccggagcg agcgcgttgc tcggctcaac agcgatcgat
     2101 ttgggccgcg cagagtctcg ggttagcggg tcccgcgttt cacatggcgt ttgcgctcca
     2161 tttggagggt gccctcgacc ccctggcgct ccggtatgcg ctcgacgccg tactcgagcg
     2221 ccaccagatc ctgcgcacgc gcctgatcga gcgagacggt gagctcatgc aaacggtgga
     2281 cgccgccgct cccgtcgagc tcactccgac cgacgtctcg acaacggcgc tggccgcctt
     2341 gatcgagcgc ccgttcggtc tcgcgagcga acacccgatc cgcgttcggc tggaacggca
     2401 cggcccgagc cgccacgcgc tctacttcgt ggtgcaccac atcgcgatgg atggttggtc
     2461 gttgtccatt ctggcccggg aactgagcga ggcttatgcc gagcgagtgg cgggcgtccg
     2521 caggaccgag caacgccccg ccctgcagta ctggcagtac gccgagtggc aagcctctca
     2581 cctgaaccct gcacgggtgg ctcgagccgt tgccttcttc ggcgaggagc tcgcgggggc
     2641 ccccgcgggc aggccagtgt ttcggggtca tgagcggctg ggcccaagtc ggtatcgggg
     2701 cgcgtggcat ccgatcgagc tcgaagcgga gctaacgacc aacctgggcg cgctggcgac
     2761 ccgatgccga agctcgcttt acgcggtgtt gctcgccgcg ttctcctgtg tgctggccaa
     2821 gcacggcgcc ggtctcgatt gggtgatcgg tacggcggtg gccaatcgca cgcctgcgtc
     2881 cgtggctgac agcttgggca tgttcgcaaa cacgctgccg gtccgggttc agctcgcgtc
     2941 cgacccgcgc ctcgtcgact tcgtgcgcga ctgcgcgtcg gcggtcttgc ggtcgaagcg
     3001 gtacgaagac gtgccgatcg acatgctcgt tcaaggcctg aacaccgagg gtccatcggg
     3061 cgaactggga ggcgcgagaa gctggccctt tcaggtgttg ttcgcgtacg acgattctta
     3121 tccgtcgcag ttcgagttag ctgggctctc cacacgggtg gagcccgtct cgaccgggac
     3181 cgctaagtac gacctgacgc tcagcttggc tcgcgccggt agcgggctta cgggttggct
     3241 cgagtatgac tccgatcaat accgcgcgac ggacgtcgcg cgcctggccc aggccttccg
     3301 ggccgcgctg aaccaactgc tagctgctcc ggagacgcgc tatcgagatt tggatttact
     3361 gtctgcacaa gacagagcac tcctgttgcg ggaggaggga cacgcggcgc ccgctccgcc
     3421 gtcgggaacg ctcgtcgagc tcctcacccg agccttcggc gtgtacgccg agcgcactgc
     3481 cgtcgagcag ggcgacgaga ctctcagtta tgggcagctg gaggcgcgct cgggagctat
     3541 cgcccgcgcg ctgcgcaagg cgggcgtttc gccaggcagt cacgtcgcgg tgttcctgga
     3601 tcgcgccccc tccctgctgt gcgtgttggt tggtgtgctc cgggccggtg ccgcgtacgt
     3661 gcccctggat ccgcgctatc ccgaagccag gttgcgtagg atcctcgaac ggtcgcgagc
     3721 ctcttgcttg gtggcgagcc cgaagtggtg ggcgagaggc atggaacttg gggcccgcat
     3781 tactctcgat gcggaacagc tgctgcacgc cagcggagag gcgggcgaac tacctcaggc
     3841 cgccgcgtcg cagccggcgt atgtgctgta cacttccggg tccagcggtg agccgaaggg
     3901 cgtcgagctc agtcaaggca atgcccggag ctttgtggac tgggcgcttg ccgcatacgc
     3961 gccgcaagag ctgcgcggcg tttgggctgg aacctcgctc ggcttcgatt tgtcggtgtt
     4021 cgagctgttc gccacgcttt gcggcggcgg gaccgtgctg ctcacggaga gcccgatcga
     4081 tccccccgtc ggcaagacgc aacccacctt gatcaacacc gtgccttcgg tgttgcgcga
     4141 gttactggag acgcacgcga ttccgggctc ggtcgttacc atcaactcag cgggcgaggt
     4201 gttgccgcgc gatctgtgtc acgcgctttg gagtcgaacc gccattcgtc gtctcgtcaa
     4261 cttgtacggt cccaccgaga ccacgactta cgtgaccgcg agcgagatca ctcgcgcggc
     4321 agactcgatc gccattggcc gaccgctccc caacgcatcg gtgttgctct tggacgagtt
     4381 cggggagcgc gtccccccgg aggtgacggg cgagatttac gtggccgggg cggcggtggc
     4441 cacgggctat ctgcacgaac cgcggcagac ggccgagcgc ttcgtgcccc acgccgaaag
     4501 cggggcgcat cgcatgtacc gaaccgggga ttacgcttac cgagactcga gcggcgaact
     4561 tcactatctc ggcagacgcg acgcacagct caaggtgcgt ggacaccgcg tggaagcggc
     4621 cgagctcgaa tgcctactgc gcagctcacc taaggttcgc gacgcggtcg tggtgttgcc
     4681 ggacggatcc ggaccggcgg gttcactggt ggcctttgtg gtgcccacgg cagcagatcc
     4741 gaacttggag agcgagctgc gccaggagct ggctaagcgt ttgcccgccg cactcgtgcc
     4801 gggcgccttc tttcagcggg ataccttgcc caaaaccagc agcagtaaat atgcgcgcag
     4861 ggcgctggcg cgggaggcag gtttggcgct gctcgaacgc tcctcggcga cggcgccact
     4921 cgagggctac atcgagcaga cgctggcgca gatctggtgc cgtgtgttgg gggtcgcgca
     4981 ggtcggaccg gatcagaact tcttcgaact cgggggtcat tcgctgagcg cggtgcgcgt
     5041 ggcgtgcttg atttcccagg agctcggatc cgccgtacaa gggcaggcgc tgttcgaata
     5101 cccggtgctg cgggacttga gccgcttcat gcaaagccgc atgtactcga ccgcgggcga
     5161 ggaccaaatc gaagccctgt tgcgcgaagt ggaagcgctc tcggatgccg aagtaag
//
LOCUS       DASTKU010000195         5460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_929962, whole genome shotgun sequence.
ACCESSION   DASTKU010000195 DASTKU010000000
VERSION     DASTKU010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929962"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VFQ61_07400"
     CDS             complement(<1..816)
                     /locus_tag="VFQ61_07400"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9954311.1"
                     /translation="MRPVGLLQNRNFRLHWASVAITQTGGSFTTVALPWLVLSVTGDD
                     AFIMSTVLGTLSVPTGVFLLFGGALADRFSPLRTLWVSRATFAALMLSLAVCSALGAI
                     PISLLYAYALFLGVVIAIGIPASQALLPALVPQDDLGKANGVLFGTLHVAQVLGPFAA
                     GWLIFWGRQQAGGPAPASSAQGIAPAFAVDAGLTILALVLMALMRVDSRPAPRLRIWP
                     FIREGLDFCRRDRGICTVLIYLMLASLFLQGPLMVSLPLITKVNLGLFERTLGA"
     gene            2116..4602
                     /locus_tag="VFQ61_07405"
     CDS             2116..4602
                     /locus_tag="VFQ61_07405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9954312.1"
                     /translation="MMSKEKTYGVLSAGIASSGRRRPAAEREELRRIVRSELITISTV
                     VAIFVMALQMDKFYPLKHWLVWHYLGAWAVAGVFSLVCLAAGLPIVLRIRGYAFRTAE
                     TLLLGHAVGVVVFFLLMFVLGMAKAFYWPVFFAVPALMLAFGARFGSNYVVRTVRTLL
                     RAAPRIKGPGIPQLAIWMFAGFALLVLYSQILSPDQVSYDARWYHLGIAEEYIAEHGI
                     HRFDDGSYVGAYPQLASVLFAWIYLAPFGTFFDKVECIVHLEFVLFLWTMLGVGLVAE
                     RLVRSRLRGAFASIFLFPGIYVYDSGLHGGSDHVIAFFAPAFALTCLAVVRSNRLSDW
                     GLLGAVAAGAALTKYNAILLLPFAGLALLARPVWETYRELNTLSKGAPFAAWRASMLS
                     ALRAWSGPALAALVLLTLTAPHWLKNWVYYSNPVFPYLSRLFRYQPWEALNRDIYVQT
                     MATTPWAPHGTAGSRVAETLNGVLNFAFVPHDWPTFHGQVPVFGFLFTLSLVILPFVS
                     APLRIWTLFVAVHGGVFLWYSTQHMDRYLHVLVPWMASGSAAVLALAWRKSVAGKLPI
                     ACLVALQIVWGLDTPTLPGHAMLGKSPTLAAIELGARGYLKEYEQRFDIFNPYTKLEK
                     HLAPDGRVLLHEHHLRAGLMRAVISDDTHTEGWIAYGGFDSRHAIYEKMRGLGVTDVI
                     WSSSSWGWAGHAGDLVFFDFVRAHTQSPATEGGFFVARLNDAPELDLSRAKVAYFACQ
                     TGFEQGLYALKALDFPDQVLDVTTRPPVPEVRLGAPEDALELASRARYLVSNPKCSPT
                     LDEAVTKDFERAAHREEIELWIRNRDQLTR"
     gene            complement(4644..>5460)
                     /locus_tag="VFQ61_07410"
     CDS             complement(4644..>5460)
                     /locus_tag="VFQ61_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954313.1"
                     /translation="SGERTSKLRTAFLELGPSLIVLDNHENDAACAELMNAFADTDVC
                     FVITARRLLLSGVLVYPVTPPLVTAGLDAFPRVAGLTRLLRWSPLALDIADGMVGSGA
                     TTAAELGDYLRNAGVERVSAIEHEDDLPEVKLLVDWLWPRLPSAAHRILAVLAHLQGD
                     SAARDSLRVLAKGGRSFDAELEALIRLRLVQEPVHQRYALHAVVRYALQRRTQFSADA
                     WFEYYVSLLERNPDRLAQEQTHWFAAMDHAYVASDFERMLRVEALSRKLEGRE"
BASE COUNT         1002 a         1699 c         1664 g         1095 t
ORIGIN      
        1 cgcgcccaag gttcgttcga acagtccgag gttgaccttg gtaatgagcg gtaagctcac
       61 catcagcggc ccctggagga atagggacgc caacatcaga taaatcagca ccgtgcagat
      121 cccgcggtct cgccggcaga aatccagccc ctcccggatg aagggccaaa tgcgcaggcg
      181 cggcgccggc cgcgagtcta cccgcatcag cgccatgagc acgagggcca gaatcgtgag
      241 acccgcgtca accgcgaagg ccggcgcgat tccctgcgcg ctgcttgccg gagctgggcc
      301 tccagcttgc tggcggcccc agaatatgag ccaaccagcg gcgaaagggc cgagcacttg
      361 cgcgacgtgc aaggttccaa acaagacgcc attggcctta ccgaggtcgt cttgcggaac
      421 gagagcggga agcaacgctt gagatgctgg aattccaatc gcgatgacta cccctaaaaa
      481 tagcgcatat gcgtacagca gggatatcgg aatcgctccc agggccgagc aaactgccaa
      541 actcagcatg agcgccgcga acgtcgctcg gctgacccaa agcgtgcgca agggtgaaaa
      601 gcgatccgcg agggccccgc caaaaaggag aaacacaccg gtaggaacac tcaatgttcc
      661 aagcacggta ctcatgatga aggcatcatc gccggtaacg ctgagcacga gccaaggcaa
      721 agccacggtc gtgaatgagc caccagtttg cgtgatcgcc acgcttgccc agtgcaatct
      781 gaagtttcta ttctgtaaca atcccacggg tcgcatcgat gtgtgtcttc gaactctgcc
      841 gctaacgatt gtttcgattc aggctggctg tcatttctct gtgcactcaa tacgggctaa
      901 tcgcccccgg ccgacagctc gagctctccc gccgtcgcta ctactgggtt tgcctcttag
      961 agtcgccaac cgcctactcc ttcactcacg cattcgcttt caccccgatc ggtgaacttt
     1021 tgctgtattt gcccgacttt ttgtgcgcca ggccccgtga accgaccagc cagctttcac
     1081 catgggctcg ggccgcccat tcgcaccaac cacttcaagc gccagttctg gagctgggtg
     1141 atgtagtgag tcgacagtgt tgcaagcccg gagcagttgc cgatccggag tgttaccagt
     1201 ctggagagcg ccaatttaat cgaatgagat gggagtttga atggcctgca gaagtgcgct
     1261 cccggcaaag accgtgaatc ctccgcactc cacgtccatc atcttatgtc tgaacgcgca
     1321 ccgaacaatc aaaagaaatt ctcggtttct cggggccgcc aacactccag gatggtcgca
     1381 agccgcggcg ccgctcgcgg ccccagaaca gaccgtctgg acgacgtcct gtgcgcccgc
     1441 gagaacagag actagtcgag tggcgagtga caccgcctgt ccgacggcaa gagctcagga
     1501 gcctggtgca ttattcagcg ttgaataata tcgaatttgg atcgaacacg cgtcttgtgt
     1561 gcgcaaccac actttgccca ggggctcgag aggtccgaca tacttctcgg aaatgctcac
     1621 gacagtcgac gctgccttgc tttacagtca ccgttgtcta gctagacacc cctgcaggtt
     1681 gcccccgggc tccggccgcc cggcattgac cgagctgatg gtttgaccgc actgggcggc
     1741 gggccacggc aaagccgcgg acacggcgag cacgggcggc aagagggcga atcttgtcct
     1801 gggtactggc gctcgggctt acgtcgctcg ggcttacgtc gctcgggctt acgtcaggaa
     1861 gaggtcacga gggcggccgc attgggccat tgcgtgccac cttgcgcctg cgtgccgggc
     1921 agcatccaag cgattgcttt gccggatccc tcaaacctgc gtgctgcccc gctcgaggag
     1981 gcgcactagc gatcgcacag acgcgcctct gcgagtggtg gacgtggcac gaccggtgca
     2041 atgccccgag ctccacccgc gcggtatggg ggtggcgcta gttcgagacc ggaggccctg
     2101 tcccgagtcg gccagatgat gtcgaaagag aagacgtacg gagtcctgag cgcaggtatt
     2161 gctagctccg gcaggcgccg tcccgcggcc gagcgcgaag agctgcgccg catcgtgcgc
     2221 agcgagctaa tcacgatatc gactgtcgtc gccatcttcg tgatggctct gcagatggat
     2281 aagttctatc cgctcaagca ctggttggtc tggcattact tgggtgcatg ggccgtggcg
     2341 ggagtcttct ccttggtgtg cttggccgcg ggcctgccaa tcgtgcttcg gatccgaggc
     2401 tacgccttta gaacggccga aacactgctc ctggggcacg ccgtcggcgt ggtggtcttc
     2461 ttcctgctga tgttcgtgct cggcatggcg aaggccttct attggcctgt cttcttcgcg
     2521 gttccggccc tcatgctcgc cttcggcgcg cgcttcggct cgaactacgt ggttcgtacc
     2581 gtacgcaccc tcctgcgtgc tgcgccgcgc atcaaggggc ccgggattcc acaactcgct
     2641 atctggatgt ttgcgggctt cgccctgttg gtgctgtatt cccaaatcct cagtccagat
     2701 caggtgtcgt acgatgcgcg ctggtatcac ctggggattg cggaagagta catcgccgaa
     2761 catggcattc accgcttcga tgacggttcc tacgtcgggg cgtatccgca gctcgcgagt
     2821 gtgctgttcg cctggatcta cctggccccg ttcggcacgt tcttcgacaa ggtcgagtgc
     2881 atcgttcatc tcgagttcgt cttgttcctg tggaccatgc tcggcgtggg gctcgtcgcc
     2941 gaaaggctgg ttcgctcccg gctacgcgga gcgttcgcga gcatttttct gttccccgga
     3001 atctatgtgt acgactcggg gcttcacggg ggctcggatc acgtgatcgc gttctttgcg
     3061 ccggcattcg cgctgacgtg cttggccgtc gtccggtcta atcggttgtc ggattggggg
     3121 ctgctcgggg cggttgcggc gggcgcggcg ttgaccaagt acaacgccat tcttctgctg
     3181 cctttcgccg gattggcgct gttggctcgc ccggtctggg aaacatatcg tgagttgaac
     3241 acgctatcca agggtgcacc gttcgcagcc tggagagctt ccatgctttc ggccctgcgt
     3301 gcttggagtg ggcccgccct tgcggcgctg gtgcttctca ccttgaccgc tccgcattgg
     3361 ctgaagaact gggtctacta ttcgaacccc gtgtttccgt acctcagccg gctcttcaga
     3421 taccaaccct gggaggctct caaccgggat atctacgtgc agacgatggc gaccacgcca
     3481 tgggccccgc acggcaccgc cggctcacgc gtggcggaga ccctgaatgg ggtgttgaac
     3541 ttcgccttcg tgccgcacga ctggccgacg ttccacgggc aggtgccggt atttggattc
     3601 ctgttcaccc tctcgttggt aatcctgccg ttcgtgtcgg ctccactccg gatctggact
     3661 ctcttcgtgg ccgtccacgg cggcgtcttc ctttggtatt ccacacagca catggatcgc
     3721 tacctccacg tgctggtgcc ttggatggcc tcgggcagcg cggcggtgtt ggcgctcgcc
     3781 tggcgcaaga gtgtcgcggg caagctcccg attgcatgcc tggtagccct ccaaatcgtg
     3841 tgggggttgg acacgccgac gcttccggga cacgccatgc tcggcaagtc gcccacgctg
     3901 gccgcaatcg agctcggcgc gcgaggctat ctaaaagaat acgagcagcg cttcgacatc
     3961 ttcaatccgt acacgaagct ggagaagcac ctcgcgcccg acggtcgcgt tctgctgcac
     4021 gagcaccatc tgcgggccgg cttgatgcgc gcggtgatca gcgatgatac tcacaccgag
     4081 ggctggatcg cctacggcgg gttcgactcc cgtcacgcta tctacgagaa gatgcgcggg
     4141 ctgggggtga ccgatgtgat ttggtcgtcc agcagctggg gctgggcggg ccacgccgga
     4201 gacttggtgt tcttcgattt cgttcgagcg cacacgcaat ccccagcaac ggagggcggt
     4261 ttcttcgtgg cgcgcttgaa tgacgctccg gaactggatc tcagccgagc caaggtcgcc
     4321 tactttgcgt gccagaccgg cttcgagcag gggctatacg cgctcaaagc gttggacttc
     4381 cccgatcagg ttctagacgt gacgacccgt ccgccggtgc cggaggtgcg tttaggtgcc
     4441 ccagaggacg cactcgagct ggccagtcga gcacgatatc tcgtttcgaa tcccaagtgc
     4501 tcaccgaccc tggacgaggc cgtgaccaag gacttcgagc gagctgccca ccgcgaggaa
     4561 atcgagctct ggattagaaa ccgggatcag ttgacccgct gaaccctccc gcgattccgg
     4621 ccgaagcgct cggtattcga gccctattcc cggccctcca atttgcggct gagagcttcg
     4681 actcgaagca tgcgctcgaa gtcgctcgcg acgtaggcgt ggtccatggc ggcgaaccaa
     4741 tgggtctgct cctgcgccag gcggtccggg ttccgctcca acaaggatac gtagtactcg
     4801 aaccaagcat ccgcggagaa ttgcgttctt cgttgcaggg cgtaccgcac gacggcatgc
     4861 aaagcataac gctggtgcac gggctcttga accagccgga gccggatcaa tgcctcgagt
     4921 tctgcgtcga agcttcggcc ccctttcgcg agcacccgaa gcgaatcccg ggccgccgag
     4981 tccccttgca ggtgcgccaa gaccgccaga atacgatggg cagcgctcgg tagccgaggc
     5041 cacagccagt ccacgagcaa cttcacctcg ggtaggtcat cctcgtgttc gatcgcactg
     5101 acgcgttcga cgcctgcgtt gcgcaagtag tcgcccaact cggccgccgt cgttgctcca
     5161 gaacctacca tgccgtcggc gatgtccaag gcaagagggc tccagcgcag cagccgtgtg
     5221 aggcctgcca ctcgcggaaa cgcatctagg cccgccgtga cgagcggtgg agtgacggga
     5281 tagacgagca caccagacaa caagagtcgt cgcgccgtaa tcacgaagca aacatccgtg
     5341 tcggcgaagg cattcatgag ctctgcacaa gcggcgtcat tctcgtgatt gtcgagtacg
     5401 attaggctcg gaccgagctc gagaaaggca gtgcgaagct tggaagtgcg ctcccccgag
//
LOCUS       DASTKU010000196        18447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_259793, whole genome shotgun sequence.
ACCESSION   DASTKU010000196 DASTKU010000000
VERSION     DASTKU010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 18447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18447
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259793"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFQ61_07415"
     CDS             <1..526
                     /locus_tag="VFQ61_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954314.1"
                     /translation="LSIISTKFLRDASQVDFWVAQMLFALLRWTTRNPKNALQGVVLF
                     DEADMYLPAIRQPATKAPLESLLKRARSAGLGVLLATQSPGDLDYRCRDTIRSWFLGR
                     IKENTALNKLKPMLNGAAGDVSSKLPGQDTGEFHLARAGEVTPFSAYRSILTTEQLSE
                     QQLLAVAANQRPRR"
     gene            542..2893
                     /locus_tag="VFQ61_07420"
     CDS             542..2893
                     /locus_tag="VFQ61_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HET9954315.1"
                     /translation="MTLLKQETLFSVIDAAIAAGLECEALLLGIPRDAQASLVRSAAR
                     SPREQYLSVFSQLNDWRARGREEPPLRTLVYNMHFLVGRREQAQVFQNALVEIDGAPS
                     KVSDHGELLFTRTKDCYVERGYRVVPPVDAPLSLTFQAWRGTGAVSIIGVAAVAPLEQ
                     VIAAVKRVMAQLPAQERVEGCVVLFTDEPTDAEQVHREGLTAIPYKELARMGVREITA
                     FVDRQQAALAQEGAASGPGDENKTVSREIRAGLRESSVRVLVVKTASVRETARRIVLS
                     LTNESRDPTRAAPLLLNLSPQPCDFQALVARAFHEHRVPFSRFDFPGLLAEKAFLPVF
                     AVDSLQAVLRSGAGGDAVLQVLEKGAKVVLVTAEERPELPKELARRFRIKSPNIRTLA
                     VQTRGTKGPIPDPPGAVIPQGSSPSSSFDRGRALLVGVANYPRINRLPENVLSDARDI
                     GELLRSPTRCGYPASNVELLLDGQATAERFRRGLQRLAKQACPEDTVVVYFSGHGFLR
                     QTGTDSEAYLLPFDANLDQLERTALSASELTRLLAAIRASRLLVLLDACHAAGTAYVK
                     TANAGATFKSGLDERTYEVLGRGAGRVVMASSRANETSLILPGMRNSLFTTYLLEALS
                     GAASKQGDDVVRVLDVFHHISENVSRQRASQHPILKAQDVENNFPIALNLQAKRASLG
                     ALVSPPVLDPSPSNTSPDERPRPPLSSKARLAIKNRLLQRWDDLADYFGIPLSDKAKF
                     TQGHEGQRTLEWLEERGRLDKLREAFVYLNWGDLVAELDRHSG"
     gene            complement(2996..3952)
                     /locus_tag="VFQ61_07425"
     CDS             complement(2996..3952)
                     /locus_tag="VFQ61_07425"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9954316.1"
                     /translation="MRSFFALWSGPSNRQVSKYAYSLLGCVALACSGAESADVPELDT
                     ATADSELIGGTEFTSAQAKSMGVIKIKDGCTATLVSRRHILTAAHCLPPPVFKIAQVG
                     KVTANTAWTTVTQSFSEGHPSWYVSCALYGCRSDLTLIPPFPSDLLIVTLFADVPASF
                     GLAKIPRMNVSTNKDVWISGYGCEAGVNGPAPNPYRLKAKMVKTLSASAVNTYREEMP
                     SELTADFGAVNILTPGIAASASAASLCPGDSGGPLLLSSDPNLGPPVPRNWVLGVNAY
                     YVFKENDTTGKSVRNIHARLWDNGPVQAWLESILPASSFVNP"
     gene            4727..5932
                     /locus_tag="VFQ61_07430"
     CDS             4727..5932
                     /locus_tag="VFQ61_07430"
                     /inference="COORDINATES: protein motif:HMM:NF038134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor M domain-containing protein"
                     /protein_id="HET9954317.1"
                     /translation="MFEKRRPDCPESLRSSGAFGARGSTLGLLSGALLASSVVACSAE
                     SGREGATDRAHESSSDAIQQQVNTLLETSALFENEAGAARMQNRDQSYMRLSELLGEL
                     ASAANEAAHAGASHYHLERLSRLAGQLPVQVARVKRALRTSPPGGAPDPNLTRRLSQL
                     QWAARSLSTSIREQQYELRYASSGSLDVGQSSELQALNGPLTPSSSWYVLSEGHVDVV
                     DVGYEDDALGLSIHDESVEPAVERDPTHTILVTKGSAKVEVPDERFAFLGAVGTTVWL
                     LPQGQLEAEAAGVLWPGIATDEVESGAFVDDVVEIRFKSVVGPNGFSVFESPEDEASS
                     PLVLVDSEDGLPDALITPVSIHRHANWAFESSGVYLVRVQARGRLAEVPNNPWVSSPT
                     VTLKFVVLP"
     gene            5953..6642
                     /locus_tag="VFQ61_07435"
     CDS             5953..6642
                     /locus_tag="VFQ61_07435"
                     /inference="COORDINATES: protein motif:HMM:NF038134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor M domain-containing protein"
                     /protein_id="HET9954318.1"
                     /translation="MNGPSLFRGLVLTIGLSALSASALATDIVFEHMDIDIDYSGGAG
                     GTMTLDFRTYSPMSAGVPTNNDDYSPAGNPIIVPVSSTYVVPGSASWSCLGAPGSTVY
                     RLTQTLTAGEVWLGLNTQDVPAATFVNNKVAFQLVSVVSAPAGARFTLYSTNSFGTPT
                     FLLNSTAGACNIASYPSGGLTNNVHTHAFWAFSAPGTYVLRFKATGTLVAGLGGGTKT
                     SGDVDVTFKVQ"
     gene            6715..8058
                     /locus_tag="VFQ61_07440"
     CDS             6715..8058
                     /locus_tag="VFQ61_07440"
                     /inference="COORDINATES: protein motif:HMM:NF038134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor M domain-containing protein"
                     /protein_id="HET9954319.1"
                     /translation="MITLGLPLVLGASCSGNEGQPTKGTGGASSSGRSGAEGARPGTT
                     AGPTSGRGGKDSGASHGGASGGDESEGGGLVHAGARDHGGGAGGDAQLAQGGRAPPGT
                     GGLTARNGAGHGEAGSGGEAGAGGETDGPPEPCVYPRYPSPEEANIVLLRGHADILSF
                     RLGCPEPSLDIVVTEDFPLGFGQKVHRTLESVLIAADGEAQLELPNPAEAPGYEDLGP
                     AGKSIWLLPEVQLDAVVWPGWNAYEVPAGVVDKDTLEVTLVRAEGPGTFHAFASSLDG
                     KPDWIFHPERGISSTTIRVGAHVHMNWAFSEAGSYALTFRAKLRTRAGRVLESAERSL
                     RFFFGDLTDLPRREPTVLRIDGLQESYAPTDRISLQAAPFGAPSSAEVVWSEQCFDFQ
                     TLESSVWTKVGTGAALELPASRFPPPDRQSCQLRASLMDGDTESATSQPVLPLIQ"
     gene            8074..8835
                     /locus_tag="VFQ61_07445"
     CDS             8074..8835
                     /locus_tag="VFQ61_07445"
                     /inference="COORDINATES: protein motif:HMM:NF038134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor M domain-containing protein"
                     /protein_id="HET9954320.1"
                     /translation="MTRLPESNALHVSSRAQLHTVIQRWWRYGVAALGLTTLACSGEP
                     TRQDLGVGSAPGAECELVYREGHGDLYFATHPTDGFHAYVRAALGGSTREELHALEQL
                     CVVVPPASRERVNEFGGAPRGDAYAFLGIEAGKTFWLLPAASQAGLPWFGLSTEQVPS
                     TSYLELWMARVQGPPESQLSSWSTDALGNPSPIFSTALGLDSAQFAAGRHLHFNWAFS
                     DEGLYRVEFQAYSRRVADDTIEESAKGLLRFWVQP"
     gene            8832..9695
                     /locus_tag="VFQ61_07450"
     CDS             8832..9695
                     /locus_tag="VFQ61_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET9954321.1"
                     /translation="MSYSVSWIARIALCWALLTGCSAPSAGHQGTIGPAPVLLSASPE
                     VGATDVYPGPVPDGTGERFTIRLTFSQPMQLNSEPRLTAGERALTPKVSWSSDRNEVE
                     LMFSAGFSGIRPLPDQTRCSLDLGGFEATDGTPLAARELVFTTGSYDALLNHSCGHTF
                     FGPFATVAAAREPAAQLPNTSTTHTLYTVSLPEADAESRYGGVLRLRFAASGKYRLYF
                     DVLPEVSWLSPERDSDSEAVVPQVPSPAPQACPGIAHQLDFTAEANQDLFLGLRRPDA
                     PQLSMLIEQVP"
     gene            9882..11729
                     /locus_tag="VFQ61_07455"
     CDS             9882..11729
                     /locus_tag="VFQ61_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HET9954322.1"
                     /translation="MSFSSVESRAALPSTCSNLGEALIGHSCFHAELGPFTEVTASGT
                     GLTLEQLPNVDAVHTFYDVVLSDPLGQSALSYRVASPARAGSWAVFHDPEIPLRIVDS
                     QGQTQPVQLEAPIAGCRELALASVYELKDERYRLLLGPSVKTHARLVIENVDDFLVFS
                     GIDRDGDGYGDPRETVTTACKPEAGYAPNDLDCDDSNPDIHPGARELCDGLDQNCNGF
                     ADDAGLPCESGVGECKNRGQLACQGEAALCEAPEIAAQPETCDGRDEDCDGVDDLSEA
                     ALCGNLTQAPRCIRVLGQVRCGCSTDVDCGDATSARVCNLDTRTCVDGCFALSGRNGC
                     KPPVVCTSADPRFIGQCELIEGASVGAPSCGDGSEPCAAPRTEDGCGCSVPGKSGVPS
                     IPTLALAVGVIWAWTWRRRSTARSRNSTARRLTRFSSLLLLSALAPSCGGRVEERLPS
                     AVRAREPEHDHSHDPGPQCSPRLGAKPIEHACQHAGLGPFVEIVPLRSPTQAPDMSLV
                     HHTYEFRMLGESPPYRGYARFRTSRGGAQAFMRPAGVHIALAGSNSKLSSVEAVAPDC
                     SLFSESSVMGVVAEQEYSVEISSEQAREFNIFVEHLEAFADEAWAERCD"
     gene            12081..12977
                     /locus_tag="VFQ61_07460"
     CDS             12081..12977
                     /locus_tag="VFQ61_07460"
                     /inference="COORDINATES: protein motif:HMM:NF025602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET9954323.1"
                     /translation="MQVLFPSDPLNPREVEPDFSEECAAARAAGFSTGLIDCELVMRG
                     DAAGATVRVAREECTALYRGWMLKPACYESLYEELVRRGVALVNSPTAYRTCHYLPES
                     YRCIEQETPRSVWLRSTGAVDFRDIHELISVFDDGPLIVKDYVKSQKHYWHEACFIPR
                     ASDRVAVERVVRNFLDLQGSDLNEGLVFREFISLKILGSHPRSGMPLAAEVRTFWYDA
                     ELILAHRYWDELTAFDVELPVAHLETIAARVPSRFFTMDVAMLENGRWLVVELGDGQV
                     SGLPSSALAPRLFQEIASRQAP"
     gene            13011..13394
                     /locus_tag="VFQ61_07465"
     CDS             13011..13394
                     /locus_tag="VFQ61_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954324.1"
                     /translation="MAVPRKGWREITVCDTTFYWRTFGTDGGLKVVVVTAAAFAHGNS
                     AQQLRFTLDYDHERSPHSDGGARLYQRAAVAPSVVRLAIERAVSLLPPFTGENGQANM
                     SLPADVLAEIQACARRHAPQTPHED"
     gene            14084..15688
                     /locus_tag="VFQ61_07470"
     CDS             14084..15688
                     /locus_tag="VFQ61_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET9954325.1"
                     /translation="MRGSSVLWAFVAVPFWLIGCGDNNNSTPAPVNNQGGKTNSSSGG
                     QTAKGGATQSGGTTASGGAQPQGGSATGGLGSGGLSSGGSTASGGASGGSSAGAAGSV
                     TGGSAGAGGATGGAGGKASGGAGGGVTGGTAGAGGGVTGGAAGSGGTAGATGGAGGTA
                     GSTGTGGRTVTDHPGACKPPTTYRNLFADILGKTDAEIDQKLEDSYKSLFHGGSDATI
                     YYEVGSDQAYIKDINNNDVRSEGMSYGLMISVQMDKKAEFERLWNWTKKVMSPSGNGL
                     FGWKASPPSTLTDTGSAPDGEEYIATSLIFAAKRWNNSNYLSEAQKTLGAMISAGLFD
                     RNSKVVKFVASTGYSDPSYVLPAFYEVWACTDKTNQSFWQGAATAGRALFKKACNSQT
                     GLAPYKSQYDGTPLSGADGIFRDDAWRVVGNIMMDHNLFAVDPWQTTFAKTYAAFWAK
                     TQKQQPMPDQLNLNGTVIESHADPAKGLIAQNALVAFGVPDEDGKFFLQALWDMQPPK
                     GNYRYYDGLLYMLATLHASGRFKLYY"
     gene            complement(15822..16076)
                     /locus_tag="VFQ61_07475"
     CDS             complement(15822..16076)
                     /locus_tag="VFQ61_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954326.1"
                     /translation="MRQQEKGHTEPPQAPWLKFPHIPGSVSMGWRMGAGENYLFYTFY
                     PWWDSLPDARREEYLDATCAPADWRLMLAERAKRAARLPP"
     gene            16384..16770
                     /locus_tag="VFQ61_07480"
     CDS             16384..16770
                     /locus_tag="VFQ61_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9954327.1"
                     /translation="MTGSPIDVMFSALGDPTRRAIVERLASGDATVLELAAPFNISLP
                     AISRHLNVLEEAGLISRGRDAQRRPCQLRPEALEQVTAWAEHTRRAWEERFDRLDEYL
                     RVLQARENDATKDRKKENDHGRRKKQ"
     gene            16748..17233
                     /locus_tag="VFQ61_07485"
     CDS             16748..17233
                     /locus_tag="VFQ61_07485"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9954328.1"
                     /translation="MAEERSSKYTWALDREIVLSRVFDAPRELVFRAWTEQEHFAKWF
                     GPRGFTTTIREADMRVGGLLRFDMQAPDGKLYDNRIEFLEIKRPELLVYDHGSDKDDD
                     ADRFRVTITFDEQSDKKTVMTMRSLQPTKEQRAIKIGFGAVELGYQTLDKLAEHLRSQ
                     T"
     gene            complement(17322..>18447)
                     /locus_tag="VFQ61_07490"
     CDS             complement(17322..>18447)
                     /locus_tag="VFQ61_07490"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9954329.1"
                     /translation="AAGLIGCSTGHSYHYSKSDSYYAYTDNESIDIAIANHYQSIPAL
                     AALPFASLHLGVGAHSDADNVGLGQRYISYRKRKAGDSQYGNAIEPIQDVGQVYDTLM
                     QRISAICAKDSNQPGTDNAQLRAALERKTSLLDFRLNDIADAKRTLGMDSVHAQKLDG
                     LVEGWREVEKATNAELAALDAPSTGGNTVCPTITKPTGNAANKNSCDQLSPIADQMIA
                     LVKLAFEWDLTRVVALTLSGASSGHRWPSQGVDKAHHTLEHSNDVAGQNIMGRYFSQK
                     FALLLSALKGIDDGGGKTALFNSSVLLGMECWSDSSDGHYLKNIPFIFAGQGAGAFQT
                     GRVVNAGGRNNNDLLVSIQRASGIESDVFGLANLCKGPII"
BASE COUNT         3494 a         5709 c         5775 g         3469 t
ORIGIN      
        1 actcagcatc atcagcacca agtttctgcg tgatgcttct caggtcgact tttgggtcgc
       61 ccagatgctg tttgcgctgt tgcgttggac gacgcgcaac ccgaagaatg cattgcaggg
      121 cgtcgtgctc ttcgacgagg ctgacatgta tctgcccgcc attcggcaac cggcgaccaa
      181 agctccgctc gagagcttgc tcaagcgggc gcgctcggcg gggctcggcg tactgctagc
      241 aacacagagc ccaggagatc tggactaccg ctgccgtgac accatacgct cttggttctt
      301 gggtcgcatc aaggagaaca cggcgctgaa caagctgaag cccatgctca acggggccgc
      361 gggtgacgtg tcctccaagc tgcccgggca agacaccgga gagtttcatc tggcccgcgc
      421 gggagaggtc acaccgttca gcgcttaccg cagcatactc acgaccgagc aattgtctga
      481 gcagcagctc ttggccgtcg ccgcgaacca gcggccgcgg cggtgagcac gaggcaatcc
      541 tttgacactg ctcaaacaag aaactctctt cagtgtcatc gacgcggcga tcgccgcggg
      601 tctagagtgt gaagcgctcc tgctcggcat tcctcgagac gcgcaggctt cgctcgtaag
      661 atccgcggca aggtccccgc gtgagcaata tctatctgtt ttcagccagc tcaacgactg
      721 gagagcacgt ggccgagaag agccacccct ccgaaccttg gtgtacaaca tgcacttcct
      781 ggtcggcaga cgcgaacagg cgcaagtctt ccagaatgcg ctggtggaga tcgacggcgc
      841 accgtcaaaa gtaagcgatc acggcgagct cctctttact cgtacgaagg actgctatgt
      901 cgagcgcggc tatcgagtag ttccaccggt ggatgcgccg ctctcgctga cattccaagc
      961 ctggcgtgga acgggtgcgg tctcgattat cggggttgcc gcggtagctc cgctcgaaca
     1021 agtcatcgcc gccgtcaaaa gggtgatggc gcaactcccc gcgcaagaac gtgtggaggg
     1081 ctgcgtcgtg ctatttaccg acgaacccac ggatgccgag caagttcacc gggaaggctt
     1141 gaccgcaatt ccctataagg agctcgcgcg catgggcgtt cgagagatca cggctttcgt
     1201 cgaccgccag caggcggcgc tcgcgcaaga aggggcggct agcggcccgg gcgacgagaa
     1261 caagacggtg agtcgagaaa tccgcgcggg gctccgcgaa agctcagtgc gcgtcctcgt
     1321 cgttaaaacc gcatcggttc gagagaccgc acgtcgcatc gtcctgagtc tcaccaacga
     1381 atcgcgagat cccacgcgtg cggctccgct gctcctgaac ctgtccccac agccatgcga
     1441 ctttcaagcg ctggtggctc gtgcatttca cgaacaccga gtccccttca gccgcttcga
     1501 ctttccgggg ttgctcgccg aaaaggcgtt cctaccggtc ttcgcggtcg attcactaca
     1561 ggcagtcctc cgatctggcg ccggggggga cgcagtcctc caggttttgg agaagggggc
     1621 gaaggtcgtt ttggtgaccg cggaggaacg gccggagttg cccaaggaac tcgcgcggcg
     1681 cttccggatc aagtctccga atatccgcac gctcgcggtc cagacgaggg gcaccaaggg
     1741 cccgatccca gatccaccgg gcgccgtcat cccacaaggc agctcgccga gttcaagctt
     1801 cgaccgagga agagccttgc tcgtaggcgt ggcaaactac ccacgcatca atcgacttcc
     1861 agagaacgtc ttgagcgacg ctcgggatat cggggagttg ttacgttcgc ccacgcgctg
     1921 tgggtatccc gcgagcaacg tcgagctgtt actcgatggc caggccacgg ccgagcggtt
     1981 tcgacgcggt ttgcagcgcc tggcgaagca agcgtgtccc gaggacaccg tcgtcgtcta
     2041 cttttcaggg cacggattcc tacgtcagac cggcacggac tccgaagctt acttgctccc
     2101 tttcgacgcc aatctagacc aactcgagcg caccgcgctg agcgccagtg agctgacgcg
     2161 ccttttggcc gccatccgcg cgtcccgctt attggtgctt ctcgacgctt gtcacgcggc
     2221 cgggacggcc tacgtgaaga cggccaacgc cggggcgaca ttcaagtccg gtctcgacga
     2281 gagaacgtac gaggttctcg ggcgaggtgc gggacgagta gtcatggcct cgtcacgcgc
     2341 gaatgaaacg tcgttgatcc tccccgggat gcgcaacagc ctctttacga cgtacctctt
     2401 ggaagccctg tccggcgctg cctcgaaaca gggcgatgac gtcgtgcgcg tgctcgacgt
     2461 atttcatcac atctccgaaa acgtttcgcg acagagggcc agccaacacc ccatcttgaa
     2521 ggcccaggac gtcgaaaaca actttcccat cgccctcaac cttcaagcca aacgagcgtc
     2581 cctcggtgcg ctggtgtcgc ctccggtcct tgatcccagt ccgagcaaca cgagtccaga
     2641 cgaacgcccg cgtccgccct tgtcctctaa agcacggtta gcgatcaaga accgtttgct
     2701 ccagcgctgg gacgatctag ccgactattt cggcatcccc ttgagtgaca aggcgaagtt
     2761 cactcaaggc cacgagggcc agcgaaccct cgagtggctc gaagagcgag gtcggctaga
     2821 taagctgaga gaagccttcg tttacttgaa ctggggtgac ctcgtcgcgg agttagatcg
     2881 gcactcgggg taaccatgag gtcagatatg gggacggctg tgccgcttcg cgccggtctt
     2941 tctcgaacgg catcgtagct agacggtgat gccgttcgat gaaggagcac atacttcagg
     3001 gattcacgaa cgatgacgct ggcaggatgc tctcgagcca cgcctgcacg ggaccattgt
     3061 cccatagacg agcgtggatg ttgcgtaccg atttgccggt agtgtcgttc tccttgaaca
     3121 catagtaggc gttcacgccg agcacccagt tgcgcggaac aggggggccg aggttgggat
     3181 cggagctcaa cagcagtgga ccaccggagt cgcccgggca cagactagca gcgctcgcgc
     3241 tcgcagcgat ccccggcgtg aggatattga cggcaccaaa atccgcagtc agctcagatg
     3301 gcatctcttc ccgataagtg ttcaccgctg aagccgagag ggtcttgacc attttcgcct
     3361 tgaggcgata cggattggga gccggaccat tcactcccgc ttcgcaacca tagccgctga
     3421 tccacacgtc cttgttggtg ctgacgttca tgcgcgggat cttcgcgagt ccgaagctgg
     3481 cgggcacgtc tgcgaaaagg gtcacgatca gaaggtccga aggaaatggt gggatgaggg
     3541 tcaggtccga tcggcaaccg tacagcgcgc agctgacgta ccaactggga tggccctccg
     3601 aaaagctctg agtgaccgtg gtccaggcag tattcgcggt cacctttccg acttgagcaa
     3661 tcttgaagac aggtggcgga aggcaatgcg cagcggtgag aatgtgcctc cggctgacga
     3721 gagtcgcagt acagccgtct ttgatcttga tcacacccat ggacttggct tgcgctgacg
     3781 tgaattcggt gcctccgatg agctccgaat cggccgtcgc ggtatccaac tccgggacat
     3841 ccgcgctctc ggcaccgcta cacgcgagcg cgacgcaacc caggagagag taggcgtact
     3901 tggagacctg acgattcgaa ggaccagacc aaagggcgaa gaatgaacgc atattgcacc
     3961 tcgaaagctc gactgcctga tgtgccgcca tcatgcggca cttacgactt agcccgctga
     4021 gcccgttcga ggaccaagat ccgcagcgcc gccgaagagg gctgttcgat ccaagtgccc
     4081 agtgagctcc cggttcgcgt ttgcagtcgc cgtgccaggg cgattcccgc gccgcgccat
     4141 ccgaccagtg acgcccccga ccgcaaagag gtcaccagaa gggcgcagta acggcgctca
     4201 cttcgcccga atgacctcgg agcttcggcc tagtgctcgg ttcgacaccc accactaagc
     4261 cgcacattga acccggacaa cccggacaac tcgggcagcc cgagcagctc cagattgctc
     4321 ggggcacgcg cgcgcgtttc ccagcaccga ccagtcttcg agcgcggcgc agtccgaacc
     4381 gctgagaccg agcagaatcg caggccgaac ccattcgggg ccgcggggac ccaacctggc
     4441 cgcgagagcc tgacctattt atgcaaccgg gttgcataat agactggtcc ccgccagacg
     4501 ccgcgccgct tattcgctgg caccatcgca aatgcaattt ggttgcattt aaaatccgaa
     4561 gccgctatcg tgccccggtc aaagcaggaa ccaagcagag cgagcggcta ctcgtagcgc
     4621 gctgcagttg cgccgtggct cacctaagcg gcccgttcga ctgtggcagt gtgtggtgtt
     4681 cgagagccgc tcgatgctcg gcttgactcc ttatctggag gtcggaatgt tcgaaaagag
     4741 aaggccggat tgtcccgagt cgttgcgttc ttctggtgcc tttggcgcgc gcggatccac
     4801 tctggggctg ctgagtggcg cgctattggc cagcagcgtc gtcgcgtgtt ccgcggagtc
     4861 gggtcgtgag ggcgcgaccg accgtgccca cgagtcgagc tccgacgcga ttcaacagca
     4921 ggtaaacacg ttgttggaga ccagcgctct attcgagaat gaagcaggcg cggcccgaat
     4981 gcagaaccgg gaccaaagct acatgcgctt gagcgagcta ttgggggaac tcgcgagcgc
     5041 tgccaacgaa gcggctcacg cgggagcttc acactatcac ctcgagcgtt tgtcgcgact
     5101 cgcggggcag cttccggtgc aagtggcacg tgtaaagcgc gcattgcgca cgtcgccgcc
     5161 gggcggagcg cccgatccga acctcacgcg gcgcctgtcg cagctgcagt gggccgctcg
     5221 ctcgctgtcg acttcgattc gcgaacagca atacgagctg cgctatgcgt cctccggaag
     5281 cttggacgtg gggcagtcca gcgaacttca agctctcaat ggaccactca cccccagctc
     5341 tagctggtac gtactgagcg aagggcacgt cgacgtggtc gatgtagggt acgaggacga
     5401 cgcgctgggg ctcagcatcc acgatgagtc ggtggagccc gccgtggagc gagatccgac
     5461 tcataccatc ctggtgacga aggggtcggc caaggtcgag gtgcccgacg agcggttcgc
     5521 ctttctggga gcagtaggga caactgtctg gctcctgcct caggggcagt tggaggcgga
     5581 ggccgctggc gtgctgtggc ctgggatcgc gaccgacgag gtcgagtccg gcgcatttgt
     5641 cgatgatgtg gtcgagattc gcttcaagag cgtcgtcgga ccgaacgggt tcagtgtctt
     5701 cgagagcccc gaagacgagg cgtcttcacc tttggtgtta gtggacagcg aagatggcct
     5761 gccggatgcg ttgatcacac cggtctcgat tcatcgccac gccaactggg cgttcgaatc
     5821 gtccggagtt tatctggtgc gcgtacaggc tcggggccgg ctcgcggagg tccccaacaa
     5881 tccgtgggtc tcgtcgccca ccgttacgct gaagttcgta gttctgccct gaggattcga
     5941 ggtgaagtga tcatgaatgg accttctctt ttcagaggtt tggtgctgac catcgggctc
     6001 tcggccttgt ctgcttcggc gctcgcgacg gatatcgttt tcgagcacat ggacatcgat
     6061 atcgattact cggggggcgc cggcggaacg atgacgctcg atttccggac atacagtccg
     6121 atgtcggcgg gggtgcccac gaacaacgat gactattcac ctgccggtaa ccccatcatc
     6181 gtgccggtct cgagcacgta cgtggttccg ggttccgcaa gctggagttg cctgggcgcc
     6241 cccggatcga cggtctatcg gttgacccag actctcaccg ccggcgaggt ttggcttggt
     6301 ctcaataccc aagacgtacc cgccgcgacg ttcgtgaaca acaaggtggc ctttcagctc
     6361 gtgagcgtag tgagcgcgcc cgcgggcgcg cggttcacgc tgtactcgac taattccttc
     6421 ggcactccta cgtttctcct gaacagcacg gcgggtgctt gcaacatcgc gagttatccc
     6481 agcggaggtc tcacgaacaa cgtgcacacg cacgcgttct gggcgttcag cgccccaggc
     6541 acctatgtcc tgcgcttcaa ggccacgggc acactcgtcg caggactcgg cgggggcacc
     6601 aagacatcag gtgatgtgga tgtgaccttc aaggtccagt gagcgccgac gtagatcgga
     6661 ttgaatatag aactgaggca acgtatgaat acgcgccatc gtttgggtgc acgaatgatc
     6721 accttgggcc tgccgctggt gctcggtgcc agttgcagcg gcaatgaggg ccagcccacc
     6781 aaaggcaccg gcggagcgtc cagctcgggg cgctcggggg ccgaaggggc acgaccgggc
     6841 acaaccgctg gccccacctc ggggcgcggc ggtaaggatt caggtgcctc gcacggtggc
     6901 gcgagcggtg gagacgaatc ggaaggcggg ggtctcgtgc acgccggcgc tcgagaccac
     6961 ggcggcgggg cgggaggtga tgcgcagctc gcgcaagggg ggagagcgcc gcccggcaca
     7021 ggcgggctca cggctcgtaa tggtgcaggg cacggcgaag caggcagcgg cggtgaggcg
     7081 ggagctggcg gcgagacgga cggtcccccg gagccgtgcg tgtatccaag atatccgtcc
     7141 cctgaagaag caaacatcgt gttgttgcgg ggccacgcgg acatactgtc cttccgactc
     7201 ggttgccccg agccttcgct cgatatcgta gtaaccgagg acttcccgct cggcttcggg
     7261 cagaaagtgc accgaactct ggagagcgtg ctcatcgccg ctgacggcga agcccagctc
     7321 gaactgccga acccagccga agctccgggt tacgaggacc tcggtcccgc tggcaaatca
     7381 atctggctgc tccccgaggt gcaactcgac gcggtggttt ggccaggttg gaacgcttac
     7441 gaggtgcccg ctggtgtggt cgacaaggac acgctcgagg tcacgctggt ccgcgcggaa
     7501 gggcccggca cctttcacgc cttcgccagc tcgctcgacg gcaagcccga ttggatcttc
     7561 cacccggagc gagggatatc gagcacaacc atacgagttg gggctcacgt gcatatgaac
     7621 tgggccttca gtgaagccgg ctcctacgcc ctcactttcc gcgccaagct ccgcactcgg
     7681 gccggacgcg tgctggagag cgccgagcgg agcttgcgtt tcttcttcgg ggacctcacc
     7741 gatctgccgc gccgcgagcc cacggtgctc cgcatcgatg gtctgcagga gtcctatgcg
     7801 cccaccgatc ggatctcact tcaggccgct ccatttggcg ccccgagcag cgctgaagtc
     7861 gtttggagtg aacagtgctt cgacttccag acactcgaaa gcagcgtctg gacgaaagtg
     7921 ggaacgggcg cggcactcga gctccccgca agccggttcc ctcctcctga ccgacagagt
     7981 tgccaactcc gcgcgagctt gatggatggt gatacggagt cggcgacttc tcagccagta
     8041 ctgccattga ttcaatagct gagacgagat ccgttgactc gattgcccga atccaacgca
     8101 ctgcacgtat cgagcagggc ccaactgcac acggtaattc agcgctggtg gcggtacggg
     8161 gtggcggcgc ttggcctcac gacgctcgcg tgctcggggg agccgacccg ccaagacctc
     8221 ggggtcggtt cggctcccgg cgcggagtgc gagctggtat atcgagaggg ccacggggac
     8281 ctctacttcg ccacccatcc caccgacggg ttccatgcgt atgtaagagc ggctttgggc
     8341 ggctcgacgc gagaggagct gcacgctcta gagcagctct gcgtcgtcgt gcccccggct
     8401 agtcgcgagc gcgtgaacga gttcggcggg gccccccgcg gagatgcgta tgcgttcttg
     8461 ggtatcgaag ctggcaaaac cttctggctg ttgcccgctg cttcacaagc agggttaccc
     8521 tggtttgggc tgagcacgga gcaggttccc tcgacctcat atctggagct ttggatggct
     8581 cgggtgcagg gcccacccga gtcgcagctc tcgagctggt cgaccgacgc actcggcaat
     8641 ccgagcccaa tcttctcgac cgcgcttggg ctagacagcg cccaattcgc ggctgggcgt
     8701 caccttcact tcaattgggc tttcagtgac gaggggctgt atcgcgtgga gttccaggcc
     8761 tactcgagga gagtagctga cgacacgatc gaggagagcg ccaaagggtt actgcgtttc
     8821 tgggttcaac catgagctac tccgtatctt ggattgcgag aatcgctcta tgctgggcct
     8881 tgcttaccgg atgcagcgca ccgagcgcag gtcatcaagg tacaattggg cccgcccctg
     8941 tgctgctgtc cgcctctccc gaggttggcg cgaccgatgt ctacccaggg ccggtaccgg
     9001 acggcacggg cgaacgcttc acgatccggc tgacgttttc gcagccgatg cagctgaact
     9061 ccgagcctcg cctgaccgcg ggtgagcgag ctctcacgcc caaggtgtct tggagttccg
     9121 accgcaacga ggtggagctt atgttcagcg cgggattctc tggtattcgg cccttgcccg
     9181 accagacccg gtgctctctc gatctcggag gcttcgaggc caccgacggc acgccgctcg
     9241 ccgctcgcga gctggtcttc acgaccggga gctacgacgc gctcttgaat cactcttgcg
     9301 ggcacacctt ctttggtccg ttcgcaaccg tggctgcagc aagagagccc gcggctcaac
     9361 ttccgaacac gagcaccacc cataccttat acacggtcag tcttcctgaa gctgacgcag
     9421 aatcccgcta cggtggggtg ttgcgcttac gattcgccgc gagcgggaag tatcgcctct
     9481 acttcgacgt cctgccggag gtcagctggc tcagcccaga acgcgattct gattcggagg
     9541 ccgtggttcc gcaggtaccc tcccccgcac cgcaagcctg tccgggtatc gctcatcagc
     9601 tagatttcac tgccgaggct aatcaggatc ttttcttggg tcttcgccgg cccgacgctc
     9661 cccaactgag catgctgatc gaacaagtcc cgtgagttca acgccggagc cggaacccac
     9721 gcgaaatccc ggccccaaca acttgcaatt cgattgtaca aaccacacca agagcaacat
     9781 cgatgtttct ccagctcgag cccagtccga gggctgctcg tcgccgcagg gcactatcgc
     9841 ggttcgcgcg cctcggagcg ggcatccctc tcgcaatcgg agtgtcgttc tccagcgtgg
     9901 agtcgcgcgc cgcgctgccc tcgacgtgtt cgaacctggg cgaggcgctc atcgggcact
     9961 cctgctttca cgcggagctc ggccccttca cggaagtgac ggcaagcggc acaggcctga
    10021 ctttggagca gctgccaaac gtcgacgccg ttcacacctt ttatgacgta gtgctgtccg
    10081 acccgctggg tcagagcgcg ctgagttacc gagtggcctc cccggcgcga gccggaagct
    10141 gggcggtatt ccacgaccca gagatccccc tgcgaatcgt ggactcgcag ggtcaaactc
    10201 aaccggtaca gctcgaggca cccatcgctg gctgcaggga gctagcgctc gcgagcgtct
    10261 atgagctgaa agacgagcgc tatcgattgc tgctcggccc cagcgtgaag acgcacgcgc
    10321 ggctcgtgat cgaaaatgtg gacgatttcc tggtgttctc gggcatcgat cgtgatggcg
    10381 acgggtacgg ggaccctcga gaaaccgtaa ccaccgcttg caaaccggag gctggctacg
    10441 cgccaaacga cctcgattgt gatgattcga accccgatat tcaccccggc gctcgcgaac
    10501 tctgcgacgg gctcgaccag aactgcaacg gcttcgcgga cgacgctggg ctgccctgcg
    10561 agagcggagt cggcgagtgc aaaaaccgag gccaactggc gtgccagggc gaagcggcct
    10621 tgtgcgaggc cccggagatc gcggcccagc cggagacctg tgatggaagg gacgaggatt
    10681 gcgacggggt ggatgacctg agcgaagcgg cattgtgcgg aaacctcacg caggcgccac
    10741 gctgcattcg agtgctcggg caggtgcgct gcggctgcag cacggacgta gactgtggtg
    10801 atgcgacgag cgctcgagtt tgtaatctcg acaccagaac ttgcgtcgat gggtgcttcg
    10861 ccctgagcgg acgcaacggc tgcaaaccgc cagtcgtgtg cacatcggcg gatccacggt
    10921 tcattggcca atgtgagctc atcgagggcg ccagcgtcgg cgcaccgagc tgtggagacg
    10981 gctcggagcc gtgtgcggcg ccgcgaaccg aagacggctg cggctgcagc gtgcctggca
    11041 agtccggcgt cccctccatt cctacgctag cgctcgccgt tggcgtcatc tgggcctgga
    11101 cctggcgcag gcgcagcaca gcgcgcagtc gtaacagcac agcgcgccgc ctcacccgct
    11161 tcagttcatt gcttctactc agcgcccttg cgccaagctg cggcggacgc gtcgaggagc
    11221 ggctcccctc agccgtgcgc gctcgcgagc ctgagcacga ccactcgcat gatccaggac
    11281 ctcagtgttc cccgcggctc ggcgccaagc ccatcgagca tgcgtgtcaa cacgccgggc
    11341 tagggccttt cgtcgaaatc gtccccctgc gaagcccaac ccaagcgccg gacatgagcc
    11401 tcgtgcatca tacgtacgag ttcagaatgt tgggcgagtc cccaccttat cgcggctacg
    11461 cgcgcttccg cacctcccgc ggcggcgcgc aagccttcat gcgcccggcc ggcgtgcata
    11521 ttgcgctagc gggctcgaac tcgaagctgt cgagcgttga agcggtcgcc cccgactgca
    11581 gtctcttttc cgagtcctca gtgatgggag tggtcgccga acaagaatac tccgttgaaa
    11641 tcagctcgga acaagcgcga gagttcaaca ttttcgtcga acacctcgaa gccttcgcag
    11701 acgaagcctg ggccgagcgt tgcgattgag acgacgggca gaaccccacg accgacactc
    11761 cgacccgact ctcaatttgg ctaacggcgg gaccatcgcg cctccctgag tgctctctgg
    11821 gttcatagcc cgtgcacacc caccgacgag attgcgagca cggtggtggc cgagtccatc
    11881 acgccccgat gcacgccctg ccgctcgagc gcattctcgt cgctcatgcg gatccgatca
    11941 cgaatcggcc ggtgcaacag cttgccgagg cgtggaagtc cgctattcct tctcacccag
    12001 ggcagccagg cgtctcggtg tcgtgccata cgtcagctgg ccttgcctga agtcagtatg
    12061 cgctaccacg gctcatcgat atgcaagtcc tcttcccttc cgacccgttg aatccgcgag
    12121 aggtcgagcc ggatttctcg gaggagtgcg cagccgcgcg agccgctgga ttttcgacgg
    12181 ggctcatcga ctgcgagcta gtgatgcgag gtgatgcggc gggggcgacg gtgcgagtgg
    12241 cgcgcgaaga gtgcaccgcc ctatatcggg gctggatgct caagcctgct tgctacgagt
    12301 cgctctacga agagctagtg cggcgcggag tcgcgttggt caactcgccc accgcctacc
    12361 gtacgtgtca ctatctcccc gagagctatc gctgtataga acaggaaacg ccgcgctcgg
    12421 tctggctgag atcgacggga gcggtggact ttcgcgatat acatgagctc atatcggtct
    12481 tcgacgacgg cccgctgatc gtcaaagact acgtgaagtc gcagaagcac tactggcacg
    12541 aggcctgttt cattccgaga gcgagcgacc gcgttgcggt cgagcgcgta gtgcgaaact
    12601 tcttggacct gcagggctcc gatctcaacg agggcctcgt cttccgagag tttataagcc
    12661 tgaagatcct cgggtcgcat ccccgaagtg ggatgccgct tgcggcagaa gttcggacgt
    12721 tctggtatga cgccgagctg atcctcgctc atcgatactg ggatgaactc accgccttcg
    12781 acgtcgagct tccagtggct catctagaga ccatcgccgc ccgtgtccca agccggttct
    12841 tcacgatgga cgtggcgatg ctcgagaacg gccgctggct cgttgtcgag ttgggtgacg
    12901 ggcaggtctc gggcctgccg agcagcgcac tcgctccccg gctattccag gagatcgctt
    12961 cacggcaagc gccgtagaag ctcaccttcg cggacggatt tgggaaggca atggcggtac
    13021 cgaggaaggg ttggcgcgag atcacggttt gcgacacaac gttctactgg agaaccttcg
    13081 gaaccgatgg cgggctcaag gtagtcgtag tcaccgcggc cgcgttcgcg cacggtaact
    13141 cggctcaaca actccggttc acactcgact atgatcacga gcgctcgccg cactcggatg
    13201 gtggagcgcg cctgtatcaa agggccgccg ttgccccctc ggtagttcgc ctcgccatcg
    13261 agcgcgcggt tagcctcttg ccacccttca ccggtgagaa cggccaagcg aatatgtctc
    13321 ttcctgccga tgttttggct gaaattcaag cctgcgcgcg tcggcacgcg ccgcagactc
    13381 cccatgagga ctgagcttcg agtacctccc tccgacaact cggtcgaatc ggcctgggca
    13441 ttgaagctcg gcgcgaggca gcttcgtcgg aacctcgctc gttcgacccg ctcgttcgac
    13501 ccggggtctc ggcaatccca gacgtggacg cgcatcccca atttcggggc tcgctcgacc
    13561 cggtcgtcct gcgtttcgtg ctgtcgatgc ccgagcatcg cgcccagcaa acgggaccgc
    13621 gttgccagcg cccagcagca atatgcgcag cagggcattg cacacgcgcc tagaacctct
    13681 cattttagca acaccaagag ctggatgatc cagctggctt acgccacggt tgattcgacg
    13741 gaactacctc gctgccgcca cacacattcg tttggtgcgc gaacgaagta cgcgagtacc
    13801 gcaactctct ttcgggcgtg taaagaagag ggcttggaat gtccatgtga tgtccgactg
    13861 gccccagctc gccgcgcgcg agcgcccttt cagaggggcc caacggactt tttgcgtgat
    13921 gatgcaagcc gtattggcgc gagccagcaa aagcactcgc atgcgccacc ccacgcggtg
    13981 ctgacgctga gtgcaagtca cctagacaga acaagcgatg aagctgctca gttggcttca
    14041 gcatgacatc gcctttggct cgatatggag tacactccgc cgcatgcgag gtagtagtgt
    14101 tctttgggcc ttcgtagccg ttccgttttg gcttatcggc tgtggtgaca acaacaactc
    14161 aacgcctgcc cccgtaaaca atcagggtgg gaagacgaat agctccagcg gcggccaaac
    14221 cgccaagggc ggtgccactc aatctggcgg cactactgct tcgggcggtg cgcaaccaca
    14281 aggcggcagc gctacgggcg ggctcggctc gggcgggctt agctccggcg gcagcactgc
    14341 gagcggcggc gcttccggag gaagctctgc cggcgcagcc ggcagcgtga caggcggcag
    14401 cgcaggtgcg ggaggcgcaa ccggcggcgc tggcggaaag gcatccggcg gcgctggcgg
    14461 aggcgtcacc ggtggcaccg cgggcgcagg cgggggcgtg accggcggcg ctgccggttc
    14521 aggcggcacc gcaggagcaa ctggcggcgc tggcggaacg gcgggctcca ccggcaccgg
    14581 cggacgcacc gtcacggatc accccggcgc ctgcaagccc cccacgacct atcgcaacct
    14641 cttcgccgat atactcggca agaccgacgc ggaaatcgat cagaagctcg aagactccta
    14701 caagtctcta ttccacggcg ggtcggacgc gacgatctac tacgaggtcg gctccgacca
    14761 ggcgtacatc aaagacatca acaacaacga tgtgcgcagc gagggtatgt cctacggatt
    14821 gatgatctcc gttcaaatgg acaagaaggc ggagttcgaa cgcctttgga actggaccaa
    14881 gaaggtcatg agcccctctg gtaatggctt gtttggttgg aaggcgagtc cgccatcgac
    14941 gctcaccgat accggctctg cgccggatgg tgaggagtac attgccacgt ccctcatctt
    15001 cgccgccaag cgttggaaca actcgaacta cctatccgag gcgcaaaaga ctctcggcgc
    15061 catgatttcg gcaggtttgt tcgatcgcaa ctcgaaggtc gtcaagttcg tggcgagcac
    15121 cgggtacagc gatccctctt acgtgctccc ggcgttctat gaagtgtggg cttgcaccga
    15181 taagaccaat caatccttct ggcagggcgc cgctaccgcg gggcgcgcgc tcttcaagaa
    15241 ggcgtgcaac tcgcagaccg ggctcgctcc gtacaagtcc caatacgatg gcaccccgct
    15301 ctccggcgct gacggcatct tccgagacga tgcttggcgc gtggtgggca acatcatgat
    15361 ggaccacaac ctgttcgccg tcgatccgtg gcaaaccacc tttgccaaga cctacgcggc
    15421 gttctgggcc aagactcaga agcagcaacc gatgcccgat cagctgaact tgaacgggac
    15481 cgtaatcgag tcccacgctg atcccgcgaa gggtctgatc gcccaaaacg cactcgtggc
    15541 gttcggggtt ccggacgaag atggtaagtt cttcctccag gcgctctggg atatgcagcc
    15601 ccccaagggc aattatcgct actacgacgg cctgctctac atgctcgcaa ccctgcacgc
    15661 gagcggccgt ttcaagctgt actactgatt gcctcacgcc gtaggtctga ctgagacaac
    15721 ggacgagaac cgagagcaag tctgaaggga aacccgcggg gggcgggttt cccggccctt
    15781 ctttggtgct cgacagattg actggttgtt tggggcctat ctcaaggcgg tagtctcgcc
    15841 gctcttttgg ctcgctctgc caacatcagc cgccaatctg cgggagcaca ggtcgcgtcc
    15901 aagtactcct cgcgtcgcgc atcgggcagc gaatcccacc aagggtagaa ggtgtaaaag
    15961 agataattct cgcccgcacc cattctccaa cccatcgaga cactgcccgg aatgtgtggg
    16021 aacttgagcc agggggcttg tgggggttcc gtgtgccctt tctcctgctg ccgcatgcgc
    16081 tcacactggc gcctttcctg gcgcgtcgcc agtgctctca ggagtcgtga gctttgcata
    16141 gcagtgcggc gaacccgcac gcgtgatgag tcgcgttgtt gaaggggccg gcgcggtttt
    16201 gggggaacag cgggtgacgt ggctgcgctg acgggcgctg tgacttgcgg cgcgcccggt
    16261 acgccgcggc cacccctcag agaccggcaa atgcacgggg ccgcgacgtc gccttgacaa
    16321 ctcggcgctc gggctttatt taacaaaaac gttaaacaca aacccgctgc gaggagcctg
    16381 cttgtgactg ggtcccccat cgacgtcatg ttttctgcgc tcggagatcc gacgcggcgg
    16441 gccatcgtgg agcgcctcgc gtcaggcgac gcgaccgtgc tcgaactcgc tgcgcctttc
    16501 aacatcagcc tcccggcgat ctcgcggcac ctgaacgtgc tcgaagaggc ggggctcatc
    16561 tcgcgcgggc gagatgcgca acgccggccg tgtcagttgc gaccggaagc gctcgagcaa
    16621 gttacggctt gggccgagca cacgcggcgc gcttgggagg aacgcttcga tcgactggac
    16681 gagtatctgc gtgttttaca ggcgcgagag aacgacgcaa ccaaggatag gaaaaaggag
    16741 aacgatcatg gccgaagaaa gaagcagtaa gtacacctgg gctctcgatc gggagatcgt
    16801 gctctctcgt gtgttcgacg ccccgcgtga actcgtattt cgagcttgga cggagcagga
    16861 gcacttcgca aagtggttcg gtccgcgcgg cttcaccacg acgatccgag aagccgatat
    16921 gcgcgtcggg ggtctcttgc ggttcgatat gcaggcacca gacggcaagc tctacgacaa
    16981 ccgcatcgag tttctcgaga tcaagcgccc cgagctgctc gtgtacgatc acggctcgga
    17041 caaggacgac gacgccgacc gttttcgcgt caccattacg ttcgatgagc agagcgacaa
    17101 aaagacggtc atgacgatgc gttcgctcca gcccacgaag gagcagcgcg cgattaagat
    17161 tggcttcggc gccgtcgagc tcggctacca gacgctcgac aagctggccg agcacctgcg
    17221 ttcacagacg taatcgtacc gcacggtggt cgacgggtgc cgttgctagc gcggcacgtc
    17281 gcgacgactg catctcagct ccggctcgaa cctcttcgac tctaaatgat cggtcccttg
    17341 cagaggttcg ccaagccgaa gacgtctgac tcgatgccgg aggctcgttg aatcgagacg
    17401 agcaagtcgt tgttgttacg cccccccgcg ttgacgacgc gaccggtctg aaaggcgccc
    17461 gcgccctgtc cggcgaagat gaagggtatg ttcttgaggt agtgtccgtc cgagctatcg
    17521 ctccagcact ccattccgag caggaccgag ctgttgaata gcgctgtctt gccgccgcca
    17581 tcgtcgatgc ctttgagtgc ggagagcagc agggcgaact tttgggagaa gtagcggccc
    17641 atgatgtttt gaccggcgac gtcgttgctg tgctcgaggg tgtggtgggc tttgtcgacc
    17701 ccctgactcg gccagcggtg gccactggat gcacccgaga gggtgagcgc cacgacgcga
    17761 gtgagatccc attcgaaggc tagtttgacc agcgcgatca tttgatccgc gatcggcgag
    17821 agttgatcgc agctgttctt gttggccgca ttgcccgtgg gcttggtgat ggtggggcaa
    17881 accgtgttgc cgccggtcga gggcgcgtcg agggccgcga gttcggcatt ggtggctttc
    17941 tcgacttcgc gccacccttc cacgagaccg tcgagctttt gcgcgtgaac gctatccatg
    18001 ccgagcgtgc gcttggcatc tgcgatgtca ttgaggcgga agtcgagcag gctcgttttg
    18061 cgttcgagcg cggcgcgcag ttgcgcattg tccgtacctg gttgattgga gtctttggcg
    18121 caaatcgcgg agatccgctg catcaaggtg tcgtagactt ggccaacgtc ttgaatgggt
    18181 tcgatggcgt taccgtattg ggagtcgccc gccttgcgtt tacgatagga gatgtagcgt
    18241 tgaccgaggc ccacgttgtc ggcatccgag tgcgcgccga cgcctaaatg caggctggcg
    18301 aacggcaagg ccgccaacgc ggggatgctc tgatagtgat tggcaatggc gatgtcgatc
    18361 gactcgttat ccgtgtaggc gtagtaagag tcgctcttag agtaatgata gctatggccc
    18421 gttgaacagc cgattagccc cgccgcg
//
LOCUS       DASTKU010000197        10859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_930443, whole genome shotgun sequence.
ACCESSION   DASTKU010000197 DASTKU010000000
VERSION     DASTKU010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10859
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930443"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFQ61_07495"
     CDS             <1..487
                     /locus_tag="VFQ61_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954330.1"
                     /translation="DAAQLGAAAPASNRGIPGTPPSATLDAKNGAPQAQPSPGLAPAP
                     NLSIPTSAQEQLQAEVRCNAQVAEACESAALSVEQGLFGTKDLKRATMLRRIALTLYV
                     KQCEGGRPSSCEQLARMYERGDLKLPNPRTVQALAQRIHELCQKHGEDPVCANPRPIP
                     P"
     gene            complement(524..1324)
                     /locus_tag="VFQ61_07500"
     CDS             complement(524..1324)
                     /locus_tag="VFQ61_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954331.1"
                     /translation="MFVLLGPDFEATPEAYARLEHATGLLSYDLRSRIRAGAWSVVKT
                     LADPAEARALAAQLTEWGFRPVLLDRSIAYDPERYVVPLSGVEPDAQGLTLRLPEREM
                     RVEFSALACIVRGEVQPGRQAQRGSPSLTPGSGSFRVSGGVELTHREQQNHTFDSYQA
                     ADFHFVGVLWIGRLSLRELGSAAATPPRILEQLVDQIALQAHVRVDRASRTSSVASFV
                     EQAAPLRTMSSEPPGLREARRELGDQRFDPYSRVIGEAERNALESART"
     gene            1663..1887
                     /locus_tag="VFQ61_07505"
     CDS             1663..1887
                     /locus_tag="VFQ61_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HET9954332.1"
                     /translation="MFGDIAPFLRTVVASLVYSFIGIAMFGLCFAVIRLVTPFSIRKE
                     IEEDQNTALAILIGAVIIGISLIIAAAIHG"
     gene            1908..3575
                     /locus_tag="VFQ61_07510"
     CDS             1908..3575
                     /locus_tag="VFQ61_07510"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET9954333.1"
                     /translation="MPDPDTPSATETAAAKETAPDGSPTRVPGPRPRILLAMVLAIAT
                     SGLVYELCMAAVGSYLLGDTVHRFSLVIGTYLSALGVGAYLSRFVDRRLAMTFIDIEF
                     LTALIGGLSSPLLMLGHSWVGAWEVLFYGTVGVVGTLVGLELPLLMRVLEHRLELKEL
                     LARALTVDYIGSLLGSLAFSWLLVPKLGLMRSSIVSGLMNAAVGLASTFALRALEGVT
                     PAALWRARLRGAFVMCILLGAWLSAGRLTARADASTLPGTAILTEQTPYQRIVVTESR
                     GPQGRTDLRLFLNGHLQFSSLDERRYHEALVHPVLALAERRQRVWIGGGGDGLAAREV
                     LRHPDVERLTLVDLDPEMTALFSRHPRLSSLNEHAFEDSRLHVVNDDAFTYLRERSPT
                     FDVAILDFPDPNSYALGKLYSLEFYRLLRARLAPRGLVVIQATSPFFARQAFWCIEAT
                     LRAAGFDTLPYRIFVPSFGDWGYILAGTGALGFEGLASAREQLATAENPGSTGPSLPR
                     GLSYLTPQILRASVAFPPDSQRSPVAVNRLDNQALVGYYSAAWERFN"
     gene            3623..4363
                     /locus_tag="VFQ61_07515"
     CDS             3623..4363
                     /locus_tag="VFQ61_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9954334.1"
                     /translation="MLCLAPLGGCAESTQKSAATARTEAPSGPAKTPPYVAPAGVPQA
                     TSAQVPVDADDGVWGAPNAPVTIVVFTDLECPFCSEAHAILAALEQEVGPERLRVVVK
                     HIPLAQHAGAVPAARVAQAIRTRHGAAKFFEFLDFAFAHQAQIAQGKALELAEQLGLS
                     RAELEPVATSNAIGAEVLRDVALADRLSVAATPHLRVNGLGLTGVVSLPALRRLVATE
                     LEAAQRLRSASVPADGLYQRRVAENFAR"
     gene            4427..5821
                     /locus_tag="VFQ61_07520"
     CDS             4427..5821
                     /locus_tag="VFQ61_07520"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9954335.1"
                     /translation="MSSRVVVLALLLCAAPAWGAPPSKAPVAPPKPDPATVALERLAL
                     SIQRVTLNNGLRVVLNPDNSSPTVAVSVTYDVGSRSEGPGQSGFAHLFEHMMFQGSQN
                     VRKGQHFELVSQRGGTLNGTTSADRTNYFESLPSSEVELALFLEADRMRWLNVNAENF
                     ENQRAVVKEEYRMRVENAPYARAYLRLGEIVFADYAPYAHSTIGSMQDLDAAKLDWVT
                     AFYQRHYAPNNAVLTIAGDIDTDRTLELVQKYFGPAERKEVPAFDAPVPPPPTSGKSE
                     VVEDKNINTEGIYWGYRIPPARSADHYALELAALLLTDGESSRLHQKLVRDRAILQQV
                     SAWTDDRRGPDQFGLSGILTEKAKVAEVEAAFEAAISDLKRNLVGAEELARVKSRLKH
                     DFVFGLQSNIRRAVKLGEYEVFFGDARLLTRELSAYLAVNAEDIRRVVGQYLTPERRF
                     RILAKPTRAQGGAS"
     gene            5818..7383
                     /locus_tag="VFQ61_07525"
     CDS             5818..7383
                     /locus_tag="VFQ61_07525"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9954336.1"
                     /translation="MKASAHQDCIMPLLCFSRRSALALLTTLPLACEPQAQRPAPASP
                     TPPATASPAAPVARANALEPPPPGLSPNAPFPKIEHQRLENGLELRVVERHVHPIVEL
                     RLVVRSGSATDGEKPGLAAVAGELLKAGGVAGLSPQKLVQRADALGTDLSILTDRDST
                     RIALNVTSGDLDAALELLSKVAFAPSFQADEFQKLRAREIERVKSSARGSAGWAASML
                     LYRELFDLPTGIHPYSRMDAMPNELAQLSLADCKAWHKAQFVPSNAALVIAGDVTPQT
                     AQAAANKWFGAWKGGEAPAVSISQPFAPQARNVYIADRPGSGQSQVLVGVLGAELRSP
                     DWVGLTLANQVLGGGVSSRLFIDVREKRSLAYSTGASLNEMETGPSALVLSAGTQTPK
                     TPDTVAALLENLEKMAARAPEPSEVDTAARFLADGFVFKLETVGSLADLTAHLYARSL
                     PDEYYDDLRARWRTLTPAEVGNVAQRYYAKTPVIVVAGDGATLGPLLAQFGPVSVLDP
                     ENGFSLKRSYPKR"
     gene            8165..>10859
                     /locus_tag="VFQ61_07530"
     CDS             8165..>10859
                     /locus_tag="VFQ61_07530"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HET9954337.1"
                     /translation="MAQSTIVGQVTSVKASARAAESASTKRSTSQTKRPKKGLTVERR
                     YTQPEADPLADLVWESRQSVITNPDGSVVFKMEGAEVPSGWSQLATDIVVSKYFRKAG
                     LHGDKAKGETSVRQVVHRIAHTIRTAGEEFGGYFASKKDADAFEAELCWLMANQYGAF
                     NSPVWFNCGLYHEYGIEGSGGNWAWSEAEEDVFETPNAYGRPQCSACFIQAVEDDLMA
                     IYELVKSEARLFKYGSGTGSNFSKIRGKQEKLSGGGTSSGLMSFLEVLDRAAGATKSG
                     GTTRRAAKMVCLDVDHPEIRDFVLWKVREEKKAKALIAAGYESDFNGDAYRTVSGQNS
                     NNSVRVSDEFMRASLSDGKWQTRARTTGEVVDTFPASELWNEIAEAAWACADPGVQYD
                     GTINRWHTCPNSGKINASNPCSEYMFLDDTACNLSSLNLTKFLGEDGTFDIEGYRHAI
                     EIFFVAQEILVDLSSYPTARIARNSHDYRPLGLGYANLGTVLMLLGIPYDSEKGRAIA
                     GALTAILCGHAYATSARMAASKGPFAGYAKNREPMLRVMRAHQEAAYAINRDACPEAL
                     WRSACEDWDSAVKAGELHGYRNAQSTVLAPTGTIGLLMDCDTTGIEPDFALVKFKKLA
                     GGGYFKIVNQSVPEALRRLRYSEAQIQEIVAYVSGTNTLLGAPHVNRASLRARGLNDE
                     ELAKIENALPGVFELTLAFAPWVVGKETYDRLGVTPEKLSQPGFNLLKYLGFSEKEIE
                     EAGEVIVGRMTIEGAPYLREEHYAVFDCANRCGRIGQRYLQPMAHVKMMAAAQPFLSG
                     AISKTVNLPNDATVDDVRQIYEEGWKLGLKAVALYRDGCKASQPLSTSSKERKSEKED
                     PEAAAAETAVASALPASGSVNGAIHALEKRPAPVLRPEGTRV"
BASE COUNT         1972 a         3443 c         3489 g         1955 t
ORIGIN      
        1 cgacgcagct cagctgggcg ccgccgcgcc ggcttccaac cgcgggatac ccggcacgcc
       61 ccccagcgcc acattagatg caaagaatgg cgcgccgcag gctcaaccca gccccggcct
      121 tgctcccgca ccgaatctgt ccatccccac cagcgcccag gaacagttgc aggccgaggt
      181 gcgctgtaat gcccaagtcg cggaagcttg cgagagcgcg gctctgagcg tcgaacaagg
      241 cttgttcgga accaaagatc tgaagcgggc caccatgctg cggcgcatcg ctttgaccct
      301 ctacgtgaaa caatgtgaag gcggccgccc ctcgtcgtgc gagcagcttg cccgcatgta
      361 cgaaagaggg gatctcaagc tgccgaatcc tcgcacggtg caggccttgg cccagcgcat
      421 tcacgagctt tgtcagaagc atggtgagga cccggtgtgc gccaaccctc gccccatccc
      481 gccttgagtt caggctagca acttggatgc atttggcgcg cgactaagtt cgggcggact
      541 ctagcgcgtt gcgctcggct tcaccgatga ctcgagagta cggatcgaag cgctggtcgc
      601 cgagctcacg gcgagcctcg cgcaaacccg gtggctcgct gctcatcgtg cgtagcgggg
      661 ccgcctgttc gacgaagctc gcgacgctcg aggtgcgcga agcgcgatcc acccgcacgt
      721 gcgcttgcag agcaatctga tccaccagtt gctccaaaat gcggggcggg gtggccgcgg
      781 cgctgccgag ctcccgcaac gagagacggc cgatccaaag cacgcccacg aaatggaagt
      841 ccgcggcctg gtacgaatcg aaggtgtggt tctgctgctc gcgatgcgtg agctcgacgc
      901 ccccactcac gcgaaaggag ccgctgccgg gcgtgaggga tgggctgccg cgttgagctt
      961 gtcgccccgg ctgcacctcc ccgcggacga tacaggccaa cgccgaaaac tccacccgca
     1021 tttcgcgctc cggcaagcgt aaggtgagcc cctgcgcatc gggctcgacg cccgagagcg
     1081 gcaccacgta ccgctcgggg tcatacgcga tgctgcgatc gaggagcacc ggacggaagc
     1141 cccattccgt gagctgagcc gcaagagccc gcgcctcggc gggatcggcg agtgtcttga
     1201 cgacgctcca ggcccccgcg cgaatacgac tccgcaggtc gtaactgagc aacccggtgg
     1261 cgtgctccag acgggcatag gcttcggggg tggcctcgaa atccggaccg aggaggacga
     1321 acattggcaa cctacaagct agaacagcag ctcgaaattc aaaagccgag aaaccaagtc
     1381 tcgatcgtaa agcagagagc ccgaagagtt ggttttcgtc accaacctgt ctgacatcgg
     1441 ttccagatgc gcggctctgg ggcggccgag gtgggcaggc tctcgcgggc gatctcacgc
     1501 agacgaggcg catgcgggcg aatgggcggc gccgccggtt gcgcgggcgt tccgctcgcg
     1561 tttcggttga attgacgtaa attgcggcgt cgatccgaaa ttcggctcgg aatgaggcag
     1621 tcctgttatg gtgtgggccc gccgcagcgc ggggagcctc tcatgtttgg agacattgcg
     1681 ccttttctaa gaaccgtggt cgcctccctc gtctattcct tcattggcat cgcgatgttc
     1741 gggctctgtt ttgccgtgat tcgcttggta acgccgttct cgattcgaaa agagatcgag
     1801 gaagatcaga acacggcgtt ggcgatcttg atcggggccg tgattatcgg cattagcctg
     1861 atcatcgcag ccgccatcca cggctaagcg cgcaacctcg cctgcgcatg cccgatcccg
     1921 acaccccgtc tgcaacggaa acggcggctg caaaggaaac ggcgccagat ggaagcccca
     1981 cgcgggtgcc cgggcctcgc ccgcggatcc tcctggcaat ggtgctggcc atcgccacgt
     2041 cggggctggt gtacgagctg tgcatggccg cggtcggaag ctacttgctc ggcgacacgg
     2101 tccatcgctt ctcgctggtc atcggcacat atctatcggc gctcggtgtg ggcgcgtatc
     2161 tatcacgctt cgtggatcgt cgcctcgcga tgaccttcat cgacatcgag ttcctcaccg
     2221 ctctgatagg cggtctgagc tcgccgttgt tgatgctggg gcacagctgg gtcggcgctt
     2281 gggaggtgct tttttatggc accgtgggcg tagtggggac gctagttggg ctcgaactgc
     2341 cgctgctcat gcgcgtgctg gaacaccgcc tcgagttgaa ggagcttttg gcacgcgccc
     2401 tgaccgtgga ctacatcggg tcactcctcg ggtcactcgc gttctcgtgg ctgctcgtgc
     2461 cgaagctcgg actgatgcgc agctcgatcg tctcgggcct gatgaacgcg gccgttggcc
     2521 tggcttctac cttcgcgctg cgcgccctcg aaggggttac gccggccgcg ctctggcgcg
     2581 ctcgcctacg gggcgcgttc gtgatgtgta ttctgctggg cgcttggttg agcgcgggcc
     2641 gcctcactgc acgagcggac gcatcgacct tgcccgggac cgcaatcctg accgaacaga
     2701 cgccgtatca acggatcgtg gtcacggaaa gccgagggcc ccaaggccgc acggatctgc
     2761 ggctgttctt gaacgggcac ctgcaattct cctcgttgga cgagcgccgg tatcacgaag
     2821 ccttggtgca cccggtgctc gcgctcgcag agcgtcgcca gcgcgtgtgg attggaggtg
     2881 gcggagatgg gctcgcggcg cgcgaagtgc tgcgtcaccc ggacgtcgag cggctcacgc
     2941 tggtggattt ggatcccgaa atgaccgcgc tgttttcccg ccatccacgc ctgagctcgc
     3001 tcaacgagca cgcgttcgag gattctcggc tgcacgtcgt gaacgacgat gccttcacct
     3061 acttgcgcga acgaagcccc accttcgacg tcgccatcct cgatttcccg gatcccaaca
     3121 gctatgccct cggcaagctt tactcgctcg agttttaccg tctgttgcgt gcgcgtttgg
     3181 cgccgcgcgg cctggtggtg attcaggcaa cctcaccgtt cttcgctcga caagccttct
     3241 ggtgcatcga agccacactg cgtgctgcgg gcttcgacac cctgccctac cgcatcttcg
     3301 tgccgtcttt cggcgattgg ggttacatcc tggccggcac gggcgcgctc ggcttcgagg
     3361 gtttggcgag cgcccgcgag cagctcgcga ctgccgaaaa cccaggttct accgggccga
     3421 gcttgccccg cggcctcagc tatctcacac cgcaaatctt gagagcgagc gtggcattcc
     3481 cgcccgatag tcaacgctcg cccgtcgcgg tcaatcgcct ggataaccag gcgttggtcg
     3541 gctactactc ggcggcgtgg gaacgcttca attgagctcc atccaaggcc tgcggtccgc
     3601 gtcggccagg ctcttcctcg cgatgctctg ccttgccccg ctggggggtt gtgcagagtc
     3661 gacccagaag tccgccgcga cggcgcggac agaagctccg agtggccccg ccaagacacc
     3721 gccctacgtt gcgccggcag gcgtgccgca agccacctcc gcccaagtac ccgtcgacgc
     3781 ggacgacgga gtgtggggag ccccgaacgc gcccgtaacg atcgtcgtat tcacggactt
     3841 ggagtgccca ttttgctcgg aggctcacgc gatattggcc gcgctggagc aagaagtcgg
     3901 gccggagcgg ctcagagtcg tggtcaaaca catcccgctc gcccaacacg ccggcgcggt
     3961 gcccgcggcc cgcgtggccc aggccattcg aacgcggcac ggcgcggcga agttcttcga
     4021 gttcttggat ttcgctttcg cacatcaagc ccagatcgcg cagggcaaag ctttggagct
     4081 ggctgaacag ctcgggttat cgagggcgga gctcgagccg gtggcgacca gcaacgcaat
     4141 tggtgcggag gtgttgcgag acgtggcttt ggccgatcgc ttgagcgtcg cggcaacccc
     4201 gcatctcagg gtgaatgggc tcgggctcac gggcgtggtc tcgttgcccg cgctgcgccg
     4261 gttggtggcg accgagctgg aagcggccca gcggcttcgt tcggcttcgg ttccggccga
     4321 cggcttatac cagcgccggg tcgccgaaaa cttcgcgcgc tgaaactacg gtcgcgcccc
     4381 gccagcccgg gttacggctg ggacgatccc atggtaagcc gcgagcatgt cgtctcgcgt
     4441 cgttgtcctg gcactgctgc tctgcgcggc gcccgcttgg ggtgccccac cgtcgaaggc
     4501 cccggttgcc cctcccaagc cggatccggc gacggtagcg ctcgagcggc tggccttgtc
     4561 cattcaacgg gtgacgttga acaacggcct acgcgtcgtg ctcaaccccg acaacagctc
     4621 gcctaccgtg gcggtgagcg tcacgtacga cgtgggcagc cgttccgaag gcccaggcca
     4681 gagcggcttc gcgcatctgt tcgagcacat gatgttccag ggcagccaga acgtccgcaa
     4741 gggccagcac ttcgaactcg tgagtcaacg cggcggcacc ttgaacggaa ccacgtctgc
     4801 ggaccgcacc aactacttcg agtccctccc ctccagcgaa gtcgagctcg cgttgttcct
     4861 cgaagcggat cgcatgcgct ggctaaacgt caacgccgag aatttcgaga accagcgcgc
     4921 ggtcgtcaag gaagagtacc ggatgcgcgt cgagaacgcg ccgtacgcgc gtgcgtacct
     4981 gcgtctcggg gagatcgtct tcgccgatta cgcgccgtac gcccactcca cgattggcag
     5041 catgcaggat ctggatgccg ccaagctcga ctgggtgacg gcgttctatc aacggcatta
     5101 cgcacccaac aacgcggtgc tcacgatcgc cggggacatc gataccgatc gcaccctcga
     5161 gctggtgcag aagtacttcg gccccgcgga gcgcaaagag gtgcccgcgt tcgatgcccc
     5221 cgttccgcct ccaccgacga gcggcaagag cgaagtcgtc gaggacaaga acatcaacac
     5281 cgaggggatc tactggggtt atcgaattcc gccggctcgc tccgccgatc actacgcgct
     5341 cgagctcgct gcgctcttgc tgacggatgg agagagctcg cgtctgcacc agaaactagt
     5401 gcgggaccgc gccatcttgc agcaagtctc cgcctggacc gatgatcgac gcggccccga
     5461 ccagttcggg ttgtccggga ttctgaccga gaaggccaag gtggccgagg tggaggcggc
     5521 attcgaagcc gcaatctcgg atctcaaacg caatttggtg ggagcggagg agctcgcgcg
     5581 cgtgaagtcg cgcctgaagc acgatttcgt gttcgggctg cagtccaaca tccgccgcgc
     5641 cgtgaagctc ggcgagtacg aggtgttctt cggggacgcg cgcttactca cccgcgagct
     5701 ctccgcgtac ttggccgtca acgcggaaga tattcggcga gtggttggtc aatacctcac
     5761 gcccgaacgt cgtttcagaa tcttggcgaa gccaacgcgg gcccaaggag gtgcttcgtg
     5821 aaagcttccg cgcatcagga ctgcatcatg ccccttctct gcttttcacg gcgctccgcg
     5881 ctggctctcc tcacgaccct gccgctcgct tgcgagcccc aagcgcaacg accggcgccc
     5941 gcgagcccta cgcccccggc tacggcgagc ccggcggctc ccgtagctcg cgccaatgca
     6001 ctcgaaccgc cgccgccagg gttgtctccg aacgcgccgt tccccaagat tgaacaccaa
     6061 agattggaga acgggctgga gctccgggtg gtggagcgac acgttcatcc aatcgtggag
     6121 ctgcgcttgg tggtgcgttc cggcagtgcc acggacggtg aaaagccggg gctcgccgcc
     6181 gtagcggggg agctactgaa agccggtggc gttgccggct tgagtcccca gaagctcgta
     6241 caacgtgccg atgcgctcgg cacggatctc tcgatcctga ccgatcgaga ctccacgcga
     6301 attgcgttga acgtcacgag tggcgatctg gacgccgcgc tagagctctt gtccaaggtc
     6361 gcgttcgcgc ccagcttcca ggccgacgaa tttcagaagc tgcgcgcccg cgagatcgaa
     6421 cgagtcaaga gctcggcccg cggcagcgcg ggttgggccg cctcgatgct cctctatcgt
     6481 gagctcttcg atctcccaac gggcatccac ccgtactcgc gcatggatgc catgccgaac
     6541 gagctcgcgc agctcagctt ggcggactgc aaggcgtggc acaaagcgca gttcgtgccc
     6601 agcaatgcag cgctcgtcat tgcgggcgac gtcacgcccc aaacggcaca agccgctgcg
     6661 aacaagtggt ttggcgcgtg gaaaggcggc gaagcaccgg ccgtttccat ctcgcagccg
     6721 ttcgcgccgc aggctcgcaa tgtctacatt gccgaccgtc ccggcagtgg gcaaagccaa
     6781 gttctggtcg gtgtcctcgg tgccgagctg cgcagtccgg attgggtggg cctgacgctg
     6841 gcaaatcaag tgctcggcgg tggtgtttca agccgtttgt tcatcgacgt tcgcgaaaag
     6901 cgttcgcttg cgtacagcac cggcgcttcc ctgaacgaga tggagaccgg tccgagtgcg
     6961 ctagtgctct cagccggtac gcagaccccg aaaaccccgg atacggtcgc cgcgttgctc
     7021 gagaatctcg agaagatggc ggctcgcgcg cccgagccct ccgaggtcga cacggcggcc
     7081 cggttcctcg cggacggctt cgtattcaag ctcgagaccg tcggaagtct cgcggatttg
     7141 acggctcact tgtacgcgcg ctctctgccc gacgagtact acgacgacct gcgagcgcgc
     7201 tggcgcacgc tcactccagc tgaagttggc aacgtcgcgc agcgctacta cgccaaaact
     7261 cccgtaatcg tggttgctgg tgacggcgcc accctcggtc ccttgctggc gcaattcgga
     7321 cccgtgagcg tgttggatcc agaaaatgga ttctcgctga agcgcagtta tccgaagcgc
     7381 tgacacgaac gccggtcggc ttttcaatcg atggctccac cccgagccac tcgattggcc
     7441 gacgtgtatc ctccggaccg acgtccgccc agggggctac ctgtgggtta ccctgaggat
     7501 tacctgaaga ggggttccgg gtggattggg cacaagttcg ggctattctg ttcagggttt
     7561 cgaaagttca gtggtctcgc gctcttagcg gcaaacgccc aattccttgt ggttttgtgt
     7621 tgagcgggcc caagtgcttg gggtacaaag tctgcccttg ctcggtcgcc ggctgagcaa
     7681 agagacagtg gttggttcgg ttcttttccg gcactcgcat ctgacacggc gcatcatcaa
     7741 gtcatcaact ggtcttgtac cgccccggat gaaagcctga gcaacccagg ctcaagcaac
     7801 agaggcttaa gcacccgagg ctaaagcaac cgagcctcaa acaacccagg ctcaagcaac
     7861 agagcccggc gcgagcagca ggctatttgg gagagcttcg ggtcacgccc aactcgggtt
     7921 gtggggtatc tgactcagcc actcgagatt gggtttcgat catctggatt ggggtcatct
     7981 cgactgcggc gcccctgttg aggcggtgag gcgctcagct ggtctaggtg cgttcgtctg
     8041 cgtggttcgc gtgtacggag cgcggccgac gtgttgatgc gagaggcata ttggttcgaa
     8101 gcgctgaatg gcgtcgcttc gaactcgcgg tttggatttg ggattcgaag gaggctgaag
     8161 agcaatggcg caatcgacga tcgtaggtca ggtaacctca gtgaaagctt cggcccgcgc
     8221 ggcggagtcc gcgtctacga aacgcagcac gagtcaaacc aaacgtccca agaagggcct
     8281 gaccgtcgag cgccgttaca cgcagccaga agcggatccc ctcgccgatc tcgtgtggga
     8341 gagccggcag agcgtcatca cgaacccgga cgggtcggtg gtgttcaaga tggagggcgc
     8401 ggaggtgcct tccggatggt cgcagctcgc gacggacatc gtcgtgtcca agtacttccg
     8461 caaggcgggt ctgcacggcg acaaagccaa gggcgagacc agcgtgcggc aagtagttca
     8521 ccgcatcgcg cacaccatcc gtaccgcggg tgaggagttc ggcggctact tcgcgagcaa
     8581 gaaagacgcc gatgcgttcg aagccgagct ctgctggttg atggccaacc aatacggcgc
     8641 gttcaattcg ccggtgtggt tcaactgcgg gctctatcac gagtacggca tcgaaggctc
     8701 gggcggaaac tgggcctgga gcgaggcgga ggaagacgtc ttcgagacgc cgaatgccta
     8761 cggtcggccg caatgctcgg cgtgcttcat tcaagccgtc gaggatgacc tgatggcgat
     8821 ttacgagctc gtcaaatcgg aagcgcgctt gttcaagtat ggctccggca ccggcagcaa
     8881 cttcagcaag atccgcggca aacaagagaa gctctcgggc ggcggcacgt cgagcggctt
     8941 gatgagcttc ctcgaggtgc tggaccgcgc ggcgggtgcg accaagagcg gcggcacgac
     9001 tcgtcgcgcg gccaagatgg tctgcctcga cgtggatcat ccggaaatcc gtgacttcgt
     9061 gctgtggaag gtgcgcgaag aaaagaaggc caaggcgttg atcgcggcgg gctacgagag
     9121 cgacttcaac ggagacgcgt accgcaccgt cagcggtcaa aactccaaca actccgtccg
     9181 ggtttcggac gagttcatgc gtgcctcgtt gagcgacggc aagtggcaaa cgcgcgcccg
     9241 caccacgggc gaggtcgtcg acaccttccc cgcctccgag ctgtggaacg aaatcgcgga
     9301 ggcggcgtgg gcctgcgccg atcccggcgt gcagtacgat ggcacgatca atcgctggca
     9361 cacctgcccc aacagcggga aaatcaacgc ctcgaacccg tgctccgagt acatgttctt
     9421 ggacgacacg gcctgtaacc tgtcgtcgct caatctgacc aagttcctcg gcgaagatgg
     9481 cacattcgac atcgagggct accgccacgc gatcgagatc ttcttcgtgg cgcaggagat
     9541 cctggtagat ctctcctcgt atccgaccgc acgcatcgcg cgaaactcgc acgattaccg
     9601 ccccctcggc ctcggctatg cgaacctggg caccgtgctg atgctgctcg ggattccgta
     9661 cgactccgag aaggggcgcg cgatcgcggg ggcgctcacc gcaatcctgt gcgggcatgc
     9721 ctacgcgacg agcgcgcgca tggccgctag caaggggccg ttcgccggtt acgccaagaa
     9781 ccgagagccg atgctgcgcg tgatgcgagc ccatcaggag gcggcctacg cgatcaatcg
     9841 ggatgcctgc ccggaggccc tctggcgctc ggcgtgtgaa gactgggaca gcgccgtcaa
     9901 ggccggcgaa ctgcatggct atcgcaacgc ccaatccacg gtgctcgcgc cgaccggcac
     9961 gatcgggctg ttgatggact gcgataccac gggcatcgag cccgatttcg cgctcgtgaa
    10021 gttcaagaag ttggcaggcg gcggctactt caagatcgtg aatcagtcgg tgccggaggc
    10081 gctgcgccga ctccggtact ccgaagctca gattcaagaa atcgtggcct acgtctcggg
    10141 caccaacacg ctgctcggtg ccccacacgt caatcgcgcc agcctgcgcg cccgcggttt
    10201 gaacgacgag gagctcgcga agatcgagaa cgcgctgccc ggcgtgttcg agctcacgtt
    10261 ggccttcgcg ccctgggtgg tgggtaaaga aacgtacgat cgtctgggcg tcactccgga
    10321 gaagctgagc caacccggtt tcaatttgct caagtacctc ggtttctccg agaaggagat
    10381 cgaggaggcg ggtgaagtca tcgtgggacg gatgacgatc gagggcgcgc cgtacctgcg
    10441 agaagagcac tacgccgtgt tcgattgcgc caaccgttgt ggccgcatcg gccaacgcta
    10501 cttgcagccg atggcccacg tgaagatgat ggcggcagct cagccgttcc tgtcgggggc
    10561 gatcagcaag accgtgaatc tgcccaacga cgcgacggtg gacgacgttc gtcagatcta
    10621 cgaggagggt tggaagctcg gcctcaaggc agtggcgttg taccgcgatg gttgcaaggc
    10681 ctctcagccg ctatccacga gcagcaagga acgcaagtcc gagaaggaag acccggaagc
    10741 agccgccgcc gaaacggcgg tcgccagcgc gctacccgcg agtggttccg tgaacggtgc
    10801 gatccacgcg ctcgaaaagc gcccggcgcc ggtgttgcgg cccgagggca cgcgcgtac
//
LOCUS       DASTKU010000198        10796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_707372, whole genome shotgun sequence.
ACCESSION   DASTKU010000198 DASTKU010000000
VERSION     DASTKU010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10796
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707372"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFQ61_07535"
     CDS             <1..911
                     /locus_tag="VFQ61_07535"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HET9954338.1"
                     /translation="YGFRVIAWESERVRTRKLDDYVRSCAGSASEAVKQLHEYWADEQ
                     TRDFARWLCEWNRAHPLDLVHVYGIDVQDPAADRAELRQLLSRTQEVPGSWQKRKTWS
                     PLASHLGALAICERGHDDLCRVALRDFERTLDEQARSAHALESTAVIGAARIALTSYS
                     AWQARASIADEARAFEARALGMASVFLQLKSRYFPHRRSILWAHNIHVVKRHSAVRWT
                     WLGTPIETLGTRLENASSWAPRALKSWFQPSTTMGSCIWSGRRAQNCCDRTTSSTPQL
                     AGEAPRAGKHRCRPNAQWSERRGSPA"
     gene            complement(811..1557)
                     /locus_tag="VFQ61_07540"
     CDS             complement(811..1557)
                     /locus_tag="VFQ61_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954339.1"
                     /translation="MGTLSVENFEIMLLARLSVDSTRPPTPEMLATSLNPLTRRYLSR
                     VELRDAITQTLAVLLEKSEITRAPLQLSSLGRARLARALALDSVPHVRDWKSFRRKYL
                     QRLVARGTSAASGALREPGVAVLARRLGVDAAAPNKISTVVDCWIERELAFRSNRLTL
                     DQLRAALLAKETGVSPRGGLKGVASRAAAKLSGARSGKKEDMVQALAESWLIESSPTP
                     EIHASPTTARSGDSDASRHVVLRQPAVESS"
     gene            complement(1557..2378)
                     /locus_tag="VFQ61_07545"
     CDS             complement(1557..2378)
                     /locus_tag="VFQ61_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954340.1"
                     /translation="MASRESLWTAVLDRQRARRVQGVPTLTVLLGDPERSELVWSRWK
                     AISGGRVCTSTRADAHALLEDWLSEAETWSAARFHFSARAALRLAHTPQELRAQLRLC
                     GAEQIWQLAEELVGIFAYPKRLISALLQTRGTPSELASIANDSFPGALTALAEVLGAE
                     LPALMMRAPTEAAQLSTGLPCLLAFAEAAPSADLAVATTRPLFERWCAAAPAPVRTRA
                     CAGAVQVTPDRRGDALLQRLGCSVTRVTAVERRTRSERVRTPTEPSIEHRRKESP"
     gene            complement(2644..2823)
                     /locus_tag="VFQ61_07550"
     CDS             complement(2644..2823)
                     /locus_tag="VFQ61_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954341.1"
                     /translation="MAERDELEQVTEGIAREFAARVPLRVLPPPVALEPIEIRQVWHT
                     GGATVTQPCNSSVRS"
     gene            complement(2935..3642)
                     /locus_tag="VFQ61_07555"
     CDS             complement(2935..3642)
                     /locus_tag="VFQ61_07555"
                     /inference="COORDINATES: protein motif:HMM:NF028154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dinitrogenase iron-molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HET9954342.1"
                     /translation="MEQPPISNEVALRIGLASRVLPEVSAGDLIEALQETLGEQLDEQ
                     SLSQITVTHLKKAFGQAHDVDGDEENERDMRQDDIAAFKEAVRILWGENPEQENLPKL
                     DPYQPGDMPGSVRVAVASNDAEELNGHFGSCTRFLIYQVSASELRLVGVRSTLLADRS
                     DDKNAFRVGLISDCRILYVVHVGGPAAAKVIKADIHIIPVPDGGAAREVLAKLQGVIG
                     GAPPPWLKKALAQVHES"
     gene            complement(3668..4564)
                     /locus_tag="VFQ61_07560"
     CDS             complement(3668..4564)
                     /locus_tag="VFQ61_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET9954343.1"
                     /translation="MSYRLITLNPGPAMHFVVCIKQVPDSAQIRVHPVTNTIMRQGVP
                     SIINPYDLFALEEALRLRDRFGGEVTVLTMGPPMAEDALRKALTFGADRAVLLTDRFF
                     AGSDTLATTFALSAAIQRIGVLWGKPDIVFTGKQTIDGDTAQVGPGVAKRLGLTQLTY
                     VSEVEKLDTKAGTIIVSRRAEGGVQRLSSRLPVLLTVLEGINEIRRGSMADALRAARA
                     KIERWSAADAGITELAKCGLKGSPTIVKQVYAPSARAEKAELVSTEGKSFAELADAVM
                     NQIFQRKAGLESELMAARARTS"
     gene            complement(4577..4984)
                     /gene="nifW"
                     /locus_tag="VFQ61_07565"
     CDS             complement(4577..4984)
                     /gene="nifW"
                     /locus_tag="VFQ61_07565"
                     /inference="COORDINATES: protein motif:HMM:NF002009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase stabilizing/protective protein NifW"
                     /protein_id="HET9954344.1"
                     /translation="MTVLDDLRKLSAAEEFFEYLAVGYEPQVLRVARLHILRRMGEYL
                     RSESIAELEDAVVRARCRQHLETAYGDFVRSTPLEQRVFKVLKDAVAPKKKGAFVPLS
                     ALETPTTGASNGPVPAATSGRATNQDGAPKRAP"
     gene            complement(5058..6275)
                     /gene="nifS"
                     /locus_tag="VFQ61_07570"
     CDS             complement(5058..6275)
                     /gene="nifS"
                     /locus_tag="VFQ61_07570"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HET9954345.1"
                     /translation="MKPVYLDNNATTRVDPRVVEAMLPYFTEQFGNASSMHAFGSAVG
                     GALKQARQSLQALLGSEFDHEIVFTSGGTEADNTALLSALETQGARNEIVTSKVEHPA
                     ILALCKHLEESRGVKVHYIGVDSRGRLDVEAYRRALSPRTAIASIMWANNETGTLFPV
                     EKLAELAHEAGALFHTDAVQVVGKMPIDLKTTEIDLLSLSGHKLHGPKGVGALYVRRG
                     VRLRPLVRGGHQERGRRAGTENAPGVIGLGKAAEIAALHLGEEQTRVRSMRDRLERGI
                     LQSIERANLMGDPDARLPNTSNIAFEFIEGEAILLLMNRAGIAASSGSACTSGSLEPS
                     HVLRAMNVPYTCAHGAIRFSFSRDNVEEDVDRVLEQLPGIISNLRQLSPFWNNDTTHA
                     APNNTQEFRPTYA"
     gene            complement(6342..7283)
                     /gene="nifU"
                     /locus_tag="VFQ61_07575"
     CDS             complement(6342..7283)
                     /gene="nifU"
                     /locus_tag="VFQ61_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HET9954346.1"
                     /translation="MWDYSEKVKEYFFNPKNSGTLDQANGVGDVGAIACGDALRLMIK
                     VDPETEVITDARFQTFGCGSAIASSSALTELIIGKTISQAMAITNQDIADFLGGLPPE
                     KMHCSVMGYEALRAAVANYRGETWVDDHEEGALVCKCFGIDEGMIERAVRMNSLTSLE
                     QVTQFTKAMGSCGTCVEKVEGVLARVNAAMVGEGLISEDMAFDPHQVPGVRKPKAKVS
                     PLAVPVKPAANKLNTLQRIRLIEQSLEELRPFFKRDGGDCELVDVEEDRVFVRFSGAC
                     LGCQLESVTLQGVQERLIEKLGQPLRVIPVQAHGHTH"
     gene            complement(7332..7652)
                     /locus_tag="VFQ61_07580"
     CDS             complement(7332..7652)
                     /locus_tag="VFQ61_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET9954347.1"
                     /translation="MIEVTETAVNAIRNAINGASEPVAGLRIMVQSGGCAGYKYLMGL
                     VKDAEPGDVVVEQSGIKVFVDEKSVPLLSGVVLDFAVSLDGSGFTFNNPNAQASCSCG
                     KSFS"
     gene            complement(7729..8028)
                     /locus_tag="VFQ61_07585"
     CDS             complement(7729..8028)
                     /locus_tag="VFQ61_07585"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET9954348.1"
                     /translation="MSTNQTSVKVEQKLFQNRYLVDGGRPHIVVKPHTTPSKRLLAMT
                     TACPAGCYSKNDSGQVEISVDGCVECGTCRIVCADTGEIEWNYPRGGFGVAYKFG"
     gene            complement(8025..9329)
                     /locus_tag="VFQ61_07590"
     CDS             complement(8025..9329)
                     /locus_tag="VFQ61_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9954349.1"
                     /translation="MSQEHFDAIVVGAGPAGSAAAYTMAKAGLKVLQLERGEYPGAKN
                     VQGAILYSEPLEKLMPDFREDAPLERHIIEQRLWMLDDTSYVGMHYRSEDFNEERPNR
                     YTIIRTQFDKWFARKVRDAGALLLCETTVTELVRDEQGRVIGVRTDRDGEPVLADVVV
                     LGEGVNGLLGQRAGLRRELKADTVALAVKEMHFLPQEVVDARFNLKGSEGVVVEAMGT
                     VTGGMVGTGFIYTNKESLSIGIGCLVSDFVETGETPAALLERFKTHPAIKPLLEGSEC
                     KEYSGHLIPEGGYHAVPELYGDGWVVVGDAGHFVNGVHREGSNLAITTGRLAGETIAE
                     LKHAGRAMTRKNLAGFKQRLDRSFVMKDLRKYKDLPGFMHKNRHMFGLYPRLLSQATQ
                     TWFRVDGVDKLSKEKEIVSSFRKSRSIGGLLGDAFKMMRVWR"
     gene            complement(9441..10631)
                     /locus_tag="VFQ61_07595"
     CDS             complement(9441..10631)
                     /locus_tag="VFQ61_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET9954350.1"
                     /translation="MRRNVHGSRCGKDAEYRESVLAQGLLIVLAAMSKEAAQPAKPAG
                     RANTRKELPEHLKAYKHIWVFIEAEHGEIHPVSLELLGEGRKLADKLGVELAGVLLGG
                     DREQLKGLGQACFECGADVLYLGVSPLLTEYRNEPYTKVMTELVEKFKPEILLLGATT
                     LGRDLAGAVATTLLTGLTADCTALAIDEERSLAATRPTFGGSLLCTILTLNFRPQMAT
                     VRPRVFSLPPRVSGRSGRIVEHAVNLVEENIITKVLEFIPDARLEKSQLAFADIVVAG
                     GLGLKSPENYRLVKDLAEVLGGDHGGTRPLVQKGWIHHDRQIGQTGKTIRPKLYIAAG
                     ISGAIQHRVGVEGADLIVAINTDPNATIFEFAHVGVVADAVDLLPALTAAFRARLQVN
                     RLAG"
BASE COUNT         1989 a         3614 c         3149 g         2044 t
ORIGIN      
        1 actacggttt tcgagtcatc gcctgggaga gcgagcgtgt tcgcactcga aagctggatg
       61 actatgtccg gagctgcgct ggctccgcca gcgaggccgt caagcaactt cacgagtatt
      121 gggccgacga gcagacgcgc gatttcgccc gctggctttg cgagtggaat cgcgcccacc
      181 cgctggacct agtgcacgtc tacggcatcg acgttcaaga tccagccgcc gatcgcgccg
      241 agctcagaca gctcctcagt cgaacccagg aggtgcctgg ctcatggcaa aagcgcaaaa
      301 cgtggagtcc tttggctagc cacctcggcg cgctcgcgat ctgcgaacgt gggcacgacg
      361 atctatgtcg ggtcgcgctc cgcgatttcg agcgaacgct cgacgagcag gcgcgcagcg
      421 cccatgcctt ggaatcaacc gccgtcattg gcgcggcgag aatcgcattg acgagctact
      481 cggcgtggca agcgcgggcc tccatcgccg acgaggcgcg ggcgttcgag gcgcgcgcgc
      541 tcggcatggc gagcgtattc ctgcagctga agagtcggta ttttcctcac cgtaggtcga
      601 tcctttgggc gcacaacatt catgtcgtga aacgacactc ggcagtgcgt tggacttggc
      661 tcggcactcc aatcgagaca ctcgggacgc gactcgagaa cgcttccagc tgggcgcccc
      721 gggcgctgaa atcctggttc caaccgagca ctacgatggg gtcttgtatc tggagcggtc
      781 gccgcgcgca gaactgctgt gatcgaacga ctagctcgac tccacagctg gctggcgaag
      841 caccacgtgc cgggaagcat cgctgtcgcc cgaacgcgca gtggtcggag aggcgtggat
      901 ctccggcgta ggcgagctct cgatcagcca actttcggcg agtgcctgca ccatgtcttc
      961 cttcttgcca ctgcgagcgc ccgacaactt ggccgcagcc cggctcgcca cccccttcaa
     1021 cccgccacgt ggggacacgc cggtctcctt ggccaagagc gccgccctca gctggtccag
     1081 cgtcaagcgg ttcgatctaa aggcgagttc gcgctcgatc caacaatcga cgacggtgct
     1141 gatcttgttg ggagcggcgg cgtccacgcc caagcggcgc gccagcaccg cgacccccgg
     1201 ttcccgaagc gcaccgctgg cggcgctcgt gccgcgcgcc accaaacgct gcagatactt
     1261 gcgtcggaag gacttccaat cgcgcacgtg cggcaccgaa tccagcgcca aggcgcgcgc
     1321 caagcgggcc cgcccgagcg agctcaactg cagtggagcg cgagtgatct ccgacttctc
     1381 cagcaagaca gccagcgttt gggtaatcgc atcccgcagc tcgacccgtg acaagtaacg
     1441 ccgggtcaag ggattcaagc tcgtagccag catctcggga gtgggcggcc tcgtcgagtc
     1501 cacggatagc cgggccagca acatgatttc gaagttctcg accgacaaag tgcccatcag
     1561 ggactctcct ttcggcgatg ctcgatggaa ggctccgttg gagttcgcac gcgctccgaa
     1621 cgcgtcctgc gctccaccgc cgttacccgg gtgacggagc aaccgagcct ctgcaagagc
     1681 gcatcgccgc gacgatccgg ggtaacttgc acggcccccg cacacgcgcg ggtgcgaacc
     1741 ggagcgggcg cggccgcaca ccagcgctcg aatagcggtc gcgtcgtcgc caccgccaag
     1801 tccgcgctcg gagccgcttc ggcgaacgcg agtaggcagg gcaatcccgt cgagagctgg
     1861 gcggcttccg taggcgcgcg catcatcagg gcgggcagct ccgcgcccaa gacttccgcg
     1921 agcgcggtga gcgctccggg aaacgagtca ttggcaatcg aggccaactc actcggcgtt
     1981 ccgcgcgtct gcagcagagc cgagatcagt cgcttggggt aagcgaagat cccaaccaac
     2041 tcttccgcga gctgccagat ttgctccgcc ccgcaaagcc gcagctgtgc ccgcaactcc
     2101 tgcggggtat gagcgagccg cagcgcagcg cgggcgctga agtgaaacct cgcggcggac
     2161 cacgtctctg cctccgatag ccaatcttcc agcagggcgt gcgcgtcagc gcgcgtcgat
     2221 gtgcagactc tgccgccgct gatcgctttc cagcgcgacc aaaccaactc cgaccgctca
     2281 ggatccccga gcaggaccgt caacgtgggg acgccctgca ccctacgagc acgctgccga
     2341 tccaggacgg ccgtccacag gctttccctc gaagccatca cgtccaaagg tatcggcgca
     2401 acaacacagc agcaaccgct tttgcggagg cgcggctctg agccgctgtg cgcgactatc
     2461 acacgtaccg agtcggaacc aacgatctcg cggcaacgcc gctgcaatcg agtgatcgcg
     2521 gccgcatcga acgaacactc aggttacgga ttacttcaca gcttactcag ctgactcgcc
     2581 gggctgccgg cgctcgtcgc gtcgtctgcg tctcttgccg cagacctagc cgtgccgcct
     2641 ctttcacgac ctcacggagg aattgcacgg ttgggtcacc gttgctccgc cggtgtgcca
     2701 gacctgacga atctcgatgg gctccagagc cacgggtggc ggtagcaccc gcaagggaac
     2761 gcgggccgcg aactcccgcg cgatcccttc ggtaacctgc tcgagctcgt cgcgctcggc
     2821 catcgaggtc cagtcgaatt ggccctcccg acctaacgcg actcggtaat tagcccaaag
     2881 cagtaactag cccaaagcgg aaagggccca atgccaagcg gccgacccgc gcaatcagga
     2941 ctcgtgtact tgagccaagg ccttcttcag ccacggcggc ggcgctccgc cgatcacgcc
     3001 ttgaagcttc gcaagtacct cgcgagccgc gccgccgtcc ggaaccggaa tgatgtgaat
     3061 gtcggccttg atcaccttgg ccgcggccgg cccccccacg tgaaccacgt acaggattcg
     3121 acagtcggag atcaatccca cacggaacgc attcttatcg tcagaacgat ccgcgagcaa
     3181 agtagatcgc acgcccacca gcctcagctc gctcgccgag acctgataga tcaaaaagcg
     3241 cgtgcaagaa ccgaagtgac cattcaactc ctcggcatcg ttggatgcga cggcaacgcg
     3301 caccgagccg ggcatgtcgc cgggctgata gggatccagc ttcggcaagt tctcttgctc
     3361 cggattctcg ccccaaagga tgcgcaccgc ttctttgaat gcggcaatgt catcctggcg
     3421 catatcgcgc tcgttctcct cgtcaccatc tacgtcatgc gcttgtccga acgccttctt
     3481 caaatgcgtg acggtgattt ggctcaggga ctgctcgtcg agctgctctc ccagtgtctc
     3541 ctgcagggcc tcgatcaggt cacctgcaga gacttcgggt agaacccgcg aggcgagccc
     3601 tatccgcagc gcgacttcgt tagagatcgg gggttgttcc atcgttcact ccaagaaacg
     3661 agcttgctca agaagtcctc gcgcgagcag ccatgagctc cgactcgagg cctgctttgc
     3721 gctggaaaat ttggttcatc acggcatccg ccagctcggc gaacgacttc ccctcggttg
     3781 acaccagctc cgccttctcc gcgcgagcac tcggggcgta aacctgcttg acgatcgtcg
     3841 gcgatccctt gagtccgcac ttggcgagct cggtgatgcc cgcatccgct gcgctccagc
     3901 gctcgatctt ggcccgcgcg gcgcgcagcg cgtctgccat actcccgcga cgaatctcgt
     3961 tgatgccctc tagcaccgtc agcagcacgg gcaatcgact cgacaagcgc tgcacgccgc
     4021 cctcggcgcg acgcgacacg atgatcgtgc ccgccttggt gtccagcttc tccacctcgg
     4081 acacgtacgt gagctgcgtg aggccgagcc gtttggctac gccggggcca acttgcgccg
     4141 tgtccccgtc aatcgtctgc ttgcccgtaa acacgatatc tggcttgccc caaagcacgc
     4201 caatgcgctg aatggcggcc gacagcgcaa aggtggtggc cagcgtgtcc gaacccgcga
     4261 agaaacgatc ggtcaacagc accgcgcggt cggcaccgaa agtgagcgcc ttgcgtagag
     4321 cgtcctcggc catgggtggg cccatcgtga gcaccgtcac ctcacctccg aaccgatcgc
     4381 gcaaacgcaa ggcctcctcg agcgcaaaga ggtcataagg attgatgatg ctcggcaccc
     4441 cctgacgcat gatcgtgttg gtcacggggt gaacgcgaat ctgcgcgcta tcgggaactt
     4501 gtttgatgca aaccacgaag tgcatggcgg gacctggatt gagggtgatg agccggtaag
     4561 acacgttcag atcgattcac ggagcgcgct ttggagcgcc atcctgattg gtcgcccttc
     4621 cagaggtcgc cgccggcacc ggcccattgg aagcgccggt agtaggcgtc tcgagtgcgc
     4681 tgagcggcac gaaggcgccc ttcttcttgg gggcaactgc gtccttcaac accttgaaca
     4741 cgcgctgctc cagtggcgtg ctccgcacga aatctccgta cgcggtctcg agatgctgac
     4801 gacagcgcgc tcgcaccacc gcgtcttcga gctccgcgat gctctcgctg cgcaggtact
     4861 cgcccatgcg tcgaaggata tgcaatcggg cgacgcggag gacctgaggc tcgtagccga
     4921 ccgcgagata ctcgaagaac tcctctgccg ccgatagctt tctgagatcg tccaatacgg
     4981 tcatgattcc tcgattcttg gttgcctcgc gcgcaaggcc ttcggtgagc caagccactc
     5041 accacccgtg accggcctca cgcgtacgtg ggtcggaact cctgagtgtt gttcggggcc
     5101 gcgtgcgtgg tgtcgttgtt ccaaaacggc gagagctgcc gcaagttgct gatgatcccg
     5161 gggagctgct cgagcacgcg gtccacgtcc tcttcgacat tgtcgcgcga aaacgagaag
     5221 cggatggcgc cgtgcgcgca ggtatagggc acgttcatgg cgcgcaatac gtgcgatggt
     5281 tcgagcgatc cggaggtgca ggcggaacca gaggaggcgg caatccccgc tcgattcatc
     5341 agcaggagga tcgcctctcc ttcgatgaac tcgaacgcaa tattgctggt gttgggcagc
     5401 cgagcgtcgg ggtcgcccat caggtttgcg cgttcgatgc tctgcaaaat cccgcgctcg
     5461 aggcggtcgc gcatgctgcg cacgcgcgtc tgctcttctc ccaggtgcag ggcagcgatc
     5521 tcggccgctt tgcccagtcc gatcacgccc ggagcgttct cggtgcccgc gcggcggccc
     5581 cgctcctgat gccctccgcg caccagcggg cgcaggcgaa ccccacgcct cacgtaaagc
     5641 gcgccgacgc ctttgggccc gtggagtttg tgtcccgaga gcgacagcag atcgatttcg
     5701 gtcgtcttca agtcgatcgg cattttgccg accacctgca cggcgtccgt atggaacagg
     5761 gcgccggctt cgtgggccag ctcggccagc ttctcgactg gaaacaacgt acccgtctcg
     5821 ttgttcgccc acatgatgga agcgatcgcg gtgcgtggac tcagcgctcg acggtaagcc
     5881 tcgacgtcca ggcgaccacg cgagtccacg ccgatgtagt gcaccttgac gccccgagac
     5941 tcctcgaggt gcttgcacag agcgaggatg gccgggtgct cgaccttgct ggtcacgatc
     6001 tcgttgcgcg cgccttgcgt ctccaaggcc gacaacaacg ccgtgttgtc ggcttcggtc
     6061 ccgcccgacg taaacacgat ctcgtggtcg aactcggatc cgagcagcgc ctgcaggctt
     6121 tgccgagctt gtttcagtgc gccacccacg gcggagccga aggcgtgcat cgacgaagca
     6181 ttgccgaact gctccgtaaa gtacggtagc atcgcctcca ccacgcgcgg gtccacccgc
     6241 gtggtggcgt tgttgtcgag ataaactggc ttcattttgg caccattcct ggctacggtg
     6301 gggtcttggc tcgccctgat cgagcttgga aaaatcgcaa ttcagtgcgt gtgtccgtga
     6361 gcttgtacgg ggattacacg caggggctga ccgagctttt cgatcaacct ctcttgcacg
     6421 ccctgcaacg tcacgctctc gagttggcag cccaggcatg cgccggagaa gcgaacgaat
     6481 acccgatctt cttcgacatc cacgagctca caatcgccgc cgtcacgctt gaagaacggg
     6541 cgcagctctt ccagcgattg ctcgattagg cggatacgct gcaaagtgtt cagcttgttc
     6601 gcggccggct tgaccggcac cgcgagcggg gaaaccttgg ccttcggctt gcgaacgccc
     6661 ggcacctgat gagggtcgaa cgccatgtcc tcggagatca ggccttctcc caccatggcc
     6721 gcgttgaccc gcgcgagtac gccctccacc ttctcgacgc acgtgccgca gctgcccatg
     6781 gctttcgtga actgcgtcac ttgttcgagc gaggtgagcg aattcatgcg caccgcgcgc
     6841 tcgatcatgc cctcgtcgat gccgaagcac ttgcagacga gcgccccctc ttcgtggtcg
     6901 tccacccaag tttcgccgcg atagttggcg accgcggcgc gcagcgcctc gtagcccatc
     6961 acggaacagt gcatcttctc cggcggcaaa ccgcccaaga agtcggcaat gtcttggttg
     7021 gtaatggcca tggcctggct gatggtcttg ccgatgatca gctcggtgag cgcagaagaa
     7081 gaggcaatcg ccgatccgca tccaaaggtt tggaaccgcg cgtccgtgat cacctccgtt
     7141 tccgggtcga ccttgatcat caagcggagc gcatctccac aggcgatcgc gcccacgtca
     7201 cccacgccgt tggcttgatc cagggtgccg gagttcttcg gattgaagaa gtactcttta
     7261 actttctcgg agtaatccca catgacacgt tcctcttgcg agtttgggct aagcggtgcg
     7321 ctaacgacga ctcaactgaa ggatttgccg caggagcagc tggcctgagc gttggggttg
     7381 ttgaacgtga aaccggagcc atccaggcta accgcgaagt cgagcaccac cccgctgagc
     7441 aaaggcacgc tcttctcgtc cacgaaaacc ttgattccac tctgctcgac cacgacgtcg
     7501 cccggttcgg cgtcctttac gagacccatt aggtatttgt agccggcgca gccgccggat
     7561 tggaccatga tgcgcagccc agcgaccggc tcactcgcgc cgttgatcgc attgcgaatc
     7621 gcgttcactg ccgtctcggt aacctcaatc atgagtagct ccagtctttg agtacgaaat
     7681 ccaatttgcc agttgttcga tcgacgttcg gccccgtgag ccctcggctc agccgaattt
     7741 gtaagcgacc ccgaatccgc cccgcggata attccattcg atctcgccag tgtccgcgca
     7801 cacgattcgg caggtgccac actccacgca cccgtccacc gagatctcca cttgcccgct
     7861 gtcgttcttg ctgtagcagc ccgccgggca ggcggtggtc atggccaaga gcctcttcga
     7921 tggcgtagtg tgaggcttga cgacgatgtg cggccggcca ccgtcgacga gatatcgatt
     7981 ttgaaacagc ttctgctcga ccttgacgga cgtttgattg gtgctcatcg ccagaccctc
     8041 atcattttga atgcgtcgcc caacagaccg ccgatgctgc ggctcttgcg gaacgacgag
     8101 acgatttctt tctctttgct cagcttgtcc acaccatcca cccggaacca ggtttgcgtg
     8161 gcttggctca gcaggcgcgg gtacaagccg aacatgtgcc gattcttgtg catgaatccc
     8221 ggcagatctt tgtacttccg gagatccttc atcacgaagc tccggtccaa tcgctgcttg
     8281 aagccagcca ggttcttgcg cgtcatggcg cgacccgcat gcttgagctc ggcgatggtc
     8341 tcgcccgcca gccgccccgt ggtgatcgct agattcgatc cctcgcggtg cacaccgttg
     8401 acgaagtgcc ctgcgtcgcc caccaccacc cagccgtctc catacagctc gggcaccgcg
     8461 tgatacccgc cctccggaat gaggtgcccc gagtactcct tgcattcgga accttcgagt
     8521 agcggcttga tcgccggatg cgtcttgaag cgctccagca acgcggcggg ggtttcaccg
     8581 gtctctacga agtccgagac cagacatcca ataccaatcg agagtgactc cttgttggta
     8641 tagatgaagc ccgtacccac catgcctccc gtcaccgtgc ccatggcctc cacgacgacg
     8701 ccctcgctcc ctttcaaatt gaagcgcgcg tcgaccactt cctgcggcag aaagtgcatt
     8761 tccttcactg ccaaagccac cgtgtcggcc ttcagttcac ggcgtagccc ggcgcgttga
     8821 ccgagtagtc cgttcacgcc ttcgccaagc acgaccacgt ccgcgagcac cggctcacca
     8881 tcgcggtccg tacgcacgcc gatcacacga ccttgttcgt cccggacgag ctcggttacg
     8941 gtcgtctcac acagcagcag ggcccccgcg tcgcgcacct tccgcgcgaa ccacttgtcg
     9001 aactgggtgc ggatgattgt gtatcggttg gggcgctcct cgttgaagtc ttcggagcga
     9061 tagtgcatgc cgacgtaaga agtgtcgtcc agcatccaaa ggcgttgttc gatgatgtgt
     9121 cgttcgagcg gcgcatcctc gcggaagtcc ggcatcaact tctcgagcgg ctcggaatac
     9181 aggatcgccc cctgcacgtt cttcgcgccc gggtattcgc cgcgctcgag ctgcagcacc
     9241 ttcagcccgg ccttggccat cgtgtacgcg gccgcgctgc ccgccgggcc cgcgccgacc
     9301 acgattgcat cgaagtgttc ttggctcatg attggttctc gctctcgcgt ctcgcggaat
     9361 catccgcgcc atctcggact ctcggtctcg gccactccgg ctgacgatct caccgcgcgt
     9421 tgacacgcgg tggacctact tcaacccgcc aaacgattta cctgcaaacg agctcggaag
     9481 gccgccgtca gcgccggcag caagtccact gcgtccgcca ccacgcctac gtgggcaaat
     9541 tcgaagatcg tcgcgttcgg atcggtgttg atggccacga tcaagtcggc cccttcgact
     9601 ccgacgcgat gttgaatcgc ccccgagatg ccggccgcaa tatacagctt gggacgaatc
     9661 gtcttgccgg tctgaccgat ctgccgatca tgatgaatcc agcccttttg cacgagtggc
     9721 cgcgtcccgc cgtgatcacc gccgagcacc tcggccaggt ctttcaccaa tcggtagttc
     9781 tcgggcgact tgagccccaa gccaccggcc acgacgatat ccgcgaaggc cagctgcgat
     9841 ttctccaagc gcgcgtcggg aatgaactcc aacaccttcg tgatgatgtt ctcttccacc
     9901 aagttgaccg cgtgctccac gatgcgtccg ctgcgcccgg aaacgcgcgg gggcagggag
     9961 aatactcgag gtctcacggt ggccatctgc ggacgaaaat tcagagtcag gatggtgcac
    10021 aacagtgagc cgccgaaagt cgggcgagtg gcggcgagcg aacgctcctc gtcgatcgcc
    10081 agcgcggtac agtcggcggt caagcccgtg agcaacgtgg tcgcgaccgc acccgccaga
    10141 tcgcgcccca gggtagtcgc gcccaggagc aagatctccg gcttgaactt ctccacgagc
    10201 tcggtcatca ccttcgtgta cggctcgttt cgatactcgg tgagcagcgg gctcacgccg
    10261 agataaagca catcggcacc gcactcgaag caagcttgtc cgagcccctt cagctgctcc
    10321 cgatccccgc ccaaaagcac ccccgcgagt tctacgccca gcttgtcggc gagcttccga
    10381 ccttcgccca acaactccag cgaaacgggg tgaatctcac catgctctgc ctcgatgaac
    10441 acccaaatgt gcttgtacgc cttcaagtgc tcgggcagct ctttgcgagt attcgcgcgg
    10501 cctgcaggct tcgcgggttg tgcggcttcc ttgctcatgg ctgcgagtac aatcagcaac
    10561 ccttgtgcca gcaccgattc acgatattcc gcgtctttac cgcatcgcga accgtggacg
    10621 ttacgtcgca ttcccaacaa tccgtccact tcgcgacagc ccgttcagct agcgacagac
    10681 cgctcgatta ccttccaacc tgcagctcat tcagctcctt ttttgcgggg cccgccctga
    10741 aacgggcgcg tcacccgtcg cacacaatcc aatgagtcta cccagcgcgc tcccca
//
LOCUS       DASTKU010000199         8808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_39599,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000199 DASTKU010000000
VERSION     DASTKU010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8808
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39599"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1837)
                     /locus_tag="VFQ61_07600"
     CDS             complement(167..1837)
                     /locus_tag="VFQ61_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954351.1"
                     /translation="MAGAAAGGAQNSGGASAQGGSPFERCHRGAIQILIAPPPTREGT
                     FLSPYNQVQIHGNELRVTAPTDGAVEVSWTLDGRSGRVDWCPTWGETCRGCDGSCLEP
                     LLSSLPSDPLTAAEVLDAEDALFLPTGMRNVQLTCAPGACQLENLIDSENNPYLTVSV
                     STPPDAFTTMAGWGFEAVHTLSFEYGEASDCNLVSDAETPEWVCEELDVQGRSLPESL
                     LIQLHTPEGPMAVFYDPDSSYTAIAPVLDSSLNLGPSVLLRANVVIEDKEPPTVITRV
                     PPTAVYWTDDGVAAWWIYDGGAPRIEGNVFEARLSFDAVGNYVWGGASTVIGMDLYIL
                     NSPFIQDGNRALAAYQRFFILQLEWFSEAAYVPVERDTLGPRGMLSRPQLPETGDLSQ
                     VVVGKLGGGALLTGTYANAPSCRHSSGRYFALPGSCRAPRAIGSTDSGAIVIQPEQDR
                     VSVMRLDQLGYLASPILDVARRTPGSSGTFTTSAASSARATALVWADSAELGSWHFVL
                     VDNATLRPLTAVFTEPGTLPAVYPIGDDWVFVHGQTFQTMRCGPKNRN"
     gene            complement(2634..4736)
                     /locus_tag="VFQ61_07605"
     CDS             complement(2634..4736)
                     /locus_tag="VFQ61_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954352.1"
                     /translation="MADMRLEKALVMAFGSRRRLGIAVLVGMFASCSSDSPPTPSPEG
                     GAGGEGTTDGGSMSGGGGANHTGGSHTTGGMQGGNAGRGGSSSGAGGVAEGSGGNTGP
                     QEGGSESGGESAQGGNATGGSSEGGTNAGGTNAGGTNAGGATSGGASSGGATSSGGNT
                     VLGGATSGGTASSGGTTPQSPSPLERCHRGAIQILLPDREGGGPSGRAHNVISIGSTE
                     LRVQERPFSRLQLGVMQGSDSAHLDWCPSWSDTCEVCDGTCPGPSSATDWSRPLRAAD
                     VFGNPDARFLPAGVRDVQVTCNSGNCSWSIQESMAGEPSIELEVSHDLPDQRAVSTIS
                     FEFGESSDCRLVEPSDNTGWECQSIQAATPLPKSPILLKPETPYGPMAEFRDAATGYT
                     NIAPILDSSLNLGTPVPLRLNLLVEHVEEPFTELPLGSIYWTPDRIEARWEFNLPDTF
                     LRYDGFAAFDKQGNQLEFSYQQKTQLTVEFMLTPLVQIDARGAVAWRYSSRNYDYVHV
                     FVIPDGGQLGDLPVLPIGERVSMVNLARAGSRLLLLGNNAEPPSCLHSAGNYFLLPGT
                     CVGQRVIGSIDTGAIVLQPQAEALKLLRLDEYGYLTSPILEVAKAASGSEGAFVANVG
                     SNSQSTAVAWADSANSETYHWLLVDNVSLRPLTQVFTEPGSLPKVVPIAGDWVFLQGE
                     QFRTLRCGPKTSKLSLSH"
     gene            5044..5487
                     /locus_tag="VFQ61_07610"
     CDS             5044..5487
                     /locus_tag="VFQ61_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9954353.1"
                     /translation="MPPGSPTPTNVSLLYALREAADAYDDRMTPVCEAAGLTLAQFNL
                     LYLVVEEGPMSLGELAKNRRCVKSNVSYLVRSMEKDDLLALVADTGDRRVRHVRASPK
                     GLKAFRTALRGAVQLQKEVSASLGEKATASLAERLMSVAHLFDRK"
     gene            5567..5992
                     /locus_tag="VFQ61_07615"
     CDS             5567..5992
                     /locus_tag="VFQ61_07615"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9954354.1"
                     /translation="MVNRNSIERINPATLYDGSAFGMSQGAVDSSGLVFLSGQVAWDE
                     QMRVVGSTCAEQVRKALDNLEIALAAAGSDLAHVLHVRVYLRGEVADHLPEIAPIFKA
                     RLGASRPALTGLGVASLATPDTLVEIEAVARRIDTSGEP"
     gene            complement(6214..7020)
                     /locus_tag="VFQ61_07620"
     CDS             complement(6214..7020)
                     /locus_tag="VFQ61_07620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9954355.1"
                     /translation="MSTPEPPTASKFGQLRAWAQQQLRAIDREHQVESLPDGKAAWIL
                     VTAALALVIPRYFGTPSAFSRAEWLVSAFSGFPYPDLHPHLYWCFFKLLHYGVLPYLC
                     IRFVLRRRLRDHGFHFTWEPKVWLLYLGMLAIVLPLTALAARTDSFLATYPKYPNAGQ
                     SLPQLLIWDLAYAFQFLMIEFFFRGFLIFSLARYVGSLAIFIMVIPYAMIHLNKPLLE
                     CLGSIITGIVLGTVALRTRSIYGGVVVHSAVAWAMDLFAIAQKGQIHNLF"
     gene            complement(7017..8318)
                     /locus_tag="VFQ61_07625"
     CDS             complement(7017..8318)
                     /locus_tag="VFQ61_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET9954356.1"
                     /translation="MPRPELAQKIRDKSAHVVVIGIGYVGLPLLVEFARAGYRATGLD
                     SDEKKVESVRRGESYVPDVINADLTPHVQSQRLSATSDPSVIARGDAVIICVPTPLNK
                     SRDPDIRHIVAAADLIACHQHSDMLVVLESTSYPGTTTELLVPRLTRAGYELGTDIFV
                     AFSPERVDPGNRVYRTRNTPKVIGGVTANCLEMAQLLYSPVIDRLVPVSSTETAEMVK
                     LLENTFRAVNIGLVNELCMISRKFGIDAFEVVRAAATKPFGFMPFYPGPGLGGHCIPV
                     DPLYLSWRLRADKHQARFIELADVINNAMPDYVVSRAAEVLNDQSKAVRGSRLLIYGV
                     AYKRDVPDVRESPALDVIHGLIQRGAHVEYMDPLVPQIDQPPLQLNSQKPNADFAGYD
                     LVLVLTDHTALDRPRLLREARAILDTRDAFQGARAPHLHRL"
     gene            complement(8349..>8808)
                     /locus_tag="VFQ61_07630"
     CDS             complement(8349..>8808)
                     /locus_tag="VFQ61_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954357.1"
                     /translation="AVCPKSNAVKRAIQGVRERIARHGALPVPMKLRNAVTGLMRKEG
                     YGSGYRYAHDFEGGYVPGETYLPEQLVGQRFYEPSSQGLEQSIRERLARLRGPRPSAQ
                     PPAASGAEPPRADTPSAEAQSAEAQSAEAQGVDAAAQPGPSTPTEEPRES"
BASE COUNT         1715 a         2745 c         2737 g         1611 t
ORIGIN      
        1 ggtcacggcc acttcgacat ggccgcctac tctgcctatc accgtggcca actcagcgac
       61 tacgaatacc ccgaagagaa ggtcgcggag gcggccaact cgttgcccaa ggtgtagcgg
      121 ggcggaggga ggccttcggc atcgatgcct tcatattggc cgaagatcaa ttgcggttct
      181 tcggcccgca gcgcatggtt tggaaggtct ggccgtggac gaatacccaa tcatctccga
      241 tggggtagac cgccggcaga gtgcccggct cggtgaacac cgcggtcaaa ggtcggaggg
      301 tcgcgttgtc cacgagtacg aagtgccaac tcccaagctc cgcgctgtcc gcccaaacca
      361 gcgctgtcgc gcgtgcactc gacgcagcgc tcgtggtaaa cgtgcccgag ctcccggggg
      421 tacgtcgtgc gacatcgagt atcgggctcg cgagataacc cagctgatcg aggcgcatca
      481 ccgatacccg gtcctgctcc ggttggatca cgatcgcacc tgaatcggta ctcccgatcg
      541 cacgtggggc acgacaagag ccgggcagcg caaagtatct ccctgaggaa tggcggcagg
      601 atggcgcatt ggcgtacgtt cccgtcagca gcgctccgcc gcctaatttg ccgactacca
      661 cctgacttag gtcgcccgtt tcgggaagct gcggtcgcga cagcatccct cggggaccta
      721 aggtgtcgcg ctcgacaggc acgtaggccg cctccgagaa ccactccaac tggagtatga
      781 agaacctttg gtacgcagcc agggcgcgat tgccgtcctg gatgaacgga ctattcagga
      841 tatatagatc cattcctatc accgtcgagg ccccacccca aacgtaattg cccactgcat
      901 caaacgaaag acgggcttca aaaacattgc cctctatccg gggagctcca ccgtcgtaga
      961 tccaccacgc cgcaacgccg tcgtctgtcc agtaaactgc cgtcggtggg acccgtgtga
     1021 tcacggtcgg aggctctttg tcttcgatca ccacgttggc ccgaaggagc acgctcgggc
     1081 caagattcag agaagaatcc aagacaggtg cgatcgccgt ataggagctg tctggatcat
     1141 agaacaccgc catcgggccc tccggggtgt gaagctgaat gagcagcgac tcgggcaaag
     1201 agcgaccttg aacgtcgagc tcttcacaaa cccactcggg tgtttcggca tcagagacga
     1261 ggttgcagtc gctagcttcc ccgtactcga aggagagagt atggaccgct tcgaatcccc
     1321 atccggccat ggttgtaaac gcgtccggcg gagtactcac gctgacggta agatatgggt
     1381 tgttctccga atcaatgagg ttctccaatt gacaggcgcc cggagcacac gtgagttgga
     1441 cgttccgcat tccggtcggc aggaatagag catcctccgc gtccaacact tcggccgccg
     1501 tgagcgggtc cgagggtaga ctagacagca gcggctctaa gcacgaaccg tcgcagcctc
     1561 tgcaagtttc gccccaagtc ggacaccaat cgacacgtcc cgatcgaccg tccaacgtcc
     1621 aagagacctc caccgcgccg tccgtgggcg ccgtcacgcg gagctcattg ccgtgaatct
     1681 gcacctgatt gtacgggctc aggaaggtgc cctctctcgt tggaggggga gcgatcagga
     1741 tctgaatggc accgcgatgg catcgctcaa aaggagaacc cccttgagcg ctcgctccgc
     1801 cggagttctg cgctcctccc gctgcggcac ccgccatgga ctcggcacct cctacaggct
     1861 cggcacctcc tacaggctcg gcacctccta cagactcggc acctcctaca gactcggtgt
     1921 gaccgccgga ggactggaaa ccgccggcgc attcggtgcc accgaaaccc gccactccac
     1981 cttcgggccc accaccgctc gcggagccgg cagtattgtg cccggaactc gaagtcccgc
     2041 cgccaccgct cagccatcca acagaacccc cctgccccgc accactgcgc ccgccttcag
     2101 agggaggatc agaagaggga tttactccgc catcagaccc atgcccggat gcgcccgcat
     2161 ccaaactagt tcgacgaccc tttgagctcc cgccctgcgc agcgccgcct ggtgaacggc
     2221 tccacgttcc acccgcccca tccgccgctg aaccacccgt tgcgccaagc gcacccggct
     2281 ggatcgccgg ctccgaagcg cacgagacga ccaacccgat gaagacgcac agcccggtgc
     2341 acgttgccaa aagaggtttg aacccatcct gagcctttgc tgaactcccc atgtgtcttc
     2401 accttcaccg gcagctaaga atttgtctcc gccgaatcga gccgagcgga gccacaccga
     2461 gcatcgctca taggtgcacg ggaagcgagc aactcaatga ctcaaagtgt ttctcagcga
     2521 aacgaacctg tcgaacgaca tggcttggtc gttccaagcg gggcccaggt aacacctctg
     2581 aactacgcac cggaatcgca ccatgatgaa ggccgcagta cgcggccctt ccgtcaatgg
     2641 ctgagactta gtttcgaggt tttcggtccg cagcgcagcg ttcggaactg ctcaccttgg
     2701 agaaacaccc aatctcccgc aatcggaacg accttcggca ggctccccgg ttccgtgaac
     2761 acttgcgtga gcgggcggag cgacacgtta tcgacgagca gccaatggta agtctcggag
     2821 ttcgcggagt ccgcccacgc tacggccgta ctctgggagt tcgagcccac attcgccacg
     2881 aaggcgccct ccgaaccgga cgccgcttta gccacttcca gtatcgggct ggtcagatag
     2941 ccatattcat ccagtcgcaa cagcttcaac gcttctgcct gtggttggag cacgatcgcg
     3001 cccgtgtcga tactcccgat cacgcgttgg ccgacgcaag tgccgggcag tagaaagtag
     3061 ttgcccgcag aatgaagaca agagggtggc tctgcgttgt tgcccagcaa cagcaaacgg
     3121 cttccagcac gggccaagtt gaccatcgat acccgttcgc cgatcggtag aactggaaga
     3181 tcgccgagtt gaccaccatc agggatcaca aaaacatgca cgtagtcgta gttccgactc
     3241 gaatagcgcc acgcaaccgc accgcgcgcg tcaatctgaa cgagcggagt gagcatgaac
     3301 tccaccgtca actgcgtttt ctgctgatag ctaaactcga gttgattgcc ctgtttgtcg
     3361 aacgcggcga aaccatcgta tcggaggaaa gtatctggca aattgaactc ccaccgcgct
     3421 tcgatgcggt ctggcgtcca atagatactt ccgagaggca actcggtgaa gggctcctct
     3481 acgtgctcaa ccaacaaatt gagacgcaaa ggcactggcg tcccgagatt gagagaagaa
     3541 tccaaaatgg gcgcgatgtt cgtgtacccg gtcgccgcgt cgcggaactc cgccatcggg
     3601 ccatatggag tctccggctt gagtagaatc ggtgatttcg gaagaggggt ggccgcttgt
     3661 atgctttgac attcccaccc tgtattgtcg gacggctcga cgagtcgaca gtcactggat
     3721 tcgccgaatt cgaacgagat agtcgagact gctctttgat ccggtagatc gtgactgact
     3781 tcgagctcga tcgacggttc tcccgccatg gattcctgga tggaccagct gcaattgcct
     3841 gaattgcaag tgacctgaac gtcgcggact cctgctggca agaagcgcgc gtctggattg
     3901 ccgaacacgt ctgccgcgcg cagcggccgc gaccagtccg tcgcggagct tggcccgggg
     3961 caagtcccgt cgcacacctc acacgtgtca ctccaacttg ggcaccagtc gagatgcgcc
     4021 gaatcactgc cttgcatcac tccgagttgc agccttgaga atggcctttc ttggacgcga
     4081 agctcagtcg aaccaatcga gatgacgttg tgcgcgcgac cagagggccc tcctccttct
     4141 ctgtccggca gcaagatctg aatggccccg cgatgacagc gctcgagtgg tgaaggggac
     4201 tgcggggtcg taccgcccga agacgccgta ccgcctgacg tcgcgccgcc cagaacagta
     4261 ttgcccccag acgaggttgc tcccccagaa gaggccccac cgctagtcgc gccgcctgcg
     4321 ttcgttccgc ctgcgttcgt tccgcctgcg ttcgttccgc cttccgacga accgccggtt
     4381 gcgtttcctc cttgtgcaga ctcgccacct gattcggagc caccctcttg ggggcccgtg
     4441 ttgccaccgg agccctcagc gacacctccc gcaccggagc tcgaaccgcc ccttcccgca
     4501 tttccaccct gcatgcctcc tgtggtgtgg gagccgccgg tatggttggc tccccctccg
     4561 cccgacatgg atccaccatc cgtcgtgccc tcgccacctg caccgccttc cgggcttggc
     4621 gtgggagggg agtccgacga gcaagatgca aacatcccaa ccaggacggc gatacctagg
     4681 cgccgccgcg aaccaaaagc catcaccaaa gccttctcca gacgcatatc agccatgctg
     4741 ccctcgttcc ttcgtcagtg ggttggctct catgggttgg agcgcatcga tcgcaataac
     4801 ccgtgagtgc tcgcggtggt ccgaggatga tgaacccaac ccgaataaat cacgagtccg
     4861 gagagggcag atgggtccag ttttggatct ggcgcgctcg agtggtatcc gtggctgggc
     4921 gccacacgca tctgtgagat cgcttcgaac cgactggact gatttcgagc tgtggatgct
     4981 catcgcctgc gcacccacgc caaaaactgc gcccgccccg cgcgctcgga gtacacctcg
     5041 cggatgccgc caggctcccc cactccgacc aacgtatcgc tgctctacgc gctgcgcgag
     5101 gccgccgatg cctacgacga tcgaatgacg ccagtctgtg aggccgctgg cctcacgttg
     5161 gcccaattca atctgctgta cctggtcgtc gaggaagggc cgatgtcgct cggtgagctg
     5221 gcaaaaaacc gacgctgcgt gaaatccaat gtctcctatc tggtgcggag catggagaag
     5281 gatgatctgc tcgcgttggt cgccgatacg ggcgaccgga gggtccgcca cgtccgggcg
     5341 agccccaaag ggctcaaggc ttttcgaacc gcgctgcgcg gggccgtcca actacaaaag
     5401 gaagtgagcg cgtcgctggg agagaaggcc accgcgagct tggccgagcg cctgatgagc
     5461 gtcgcccact tgttcgaccg gaagtgacag tgctcgaatt gtgggttgcc ggccgcgtcg
     5521 caatggttca agttttgaac cgttcaaacc ttgaaccagg tgaaccatgg tcaatcgaaa
     5581 ctcaatcgag cgcatcaacc ccgcgaccct ttacgacggc tccgccttcg gcatgagcca
     5641 aggcgccgtg gattcctcgg gcctcgtctt tctctccggg caggtggctt gggacgagca
     5701 gatgcgcgtg gtgggctcca cttgcgccga acaggttcgg aaagccctcg ataacctgga
     5761 gattgcgctg gccgcggcgg gctctgatct cgctcacgtg cttcacgttc gtgtctattt
     5821 gcgcggcgag gtcgccgatc acctgcccga gattgccccc atcttcaaag cgcggctggg
     5881 agcttcgcgg cccgcactca cgggcttggg cgtcgcttcg ctcgcgaccc cagacacgtt
     5941 ggtcgaaatc gaagccgtgg cgcgtcgcat cgatacgtca ggcgagccgt aggcgcgtta
     6001 cgtgttggcc acgagataac gtgcgcgctg cgcgccgtta tctcgccgtg gcgcagacgt
     6061 ggcccgccgt tctcgcgtcg cgcaaccgcg ccggccccct ttgcttacgt atcgatcatg
     6121 ccgcttgcgc ggtgtaaaga cggacgtggg gccatagggc tgcgctcttg gtgattggaa
     6181 cgcaggtcgc gcccgtcggc tgggtgcgga caatcagaat aggttgtgaa tctggccctt
     6241 ttgggcaatc gcgaataggt ccatggccca cgccaccgca gagtggacga cgacgccacc
     6301 gtagatcgat cgggtgcgga gcgcgacggt gccgagcact atgccggtga tgatggatcc
     6361 caggcactcg agcagcggct tgttcaggtg aatcatggcg taagggatga ccatgatgaa
     6421 gatcgcgagc gagcccacgt aacgcgccag gctgaagatc agaaagccgc gaaagaagaa
     6481 ctcgatcatc aaaaactgaa acgcatacgc gaggtcccag atcaggagct gcggcagcga
     6541 ttgcccggcg ttggggtact tgggataggt cgccaagaac gagtccgtgc gcgcggcaag
     6601 agcggtgagc ggcagcacga tcgcgagcat gccgaggtaa agcagccaga ctttgggctc
     6661 ccaggtgaaa tggaagccgt gatcgcgcag ccgccggcgc agcacgaagc gaatgcacag
     6721 gtaaggcaag acgccgtagt gcagcagctt gaagaagcac caataaaggt gcgggtgcag
     6781 gtcgggatag gggaaacccg agaatgcact gaccaaccat tcggcgcggc tgaaggcgct
     6841 cggggtgccg aagtagcgcg ggatgacgag cgcgagcgcc gcggtgacga ggatccaagc
     6901 cgctttgccg tccggtaaac tttcgacttg gtgctcgcgg tcgatggcgc ggagctgctg
     6961 ttgggcccaa gcgcggagtt gtccaaactt gctcgcggtg ggaggttcgg gagtgctcat
     7021 aggcgatgaa ggtgcggcgc gcgggcgcct tggaaggcat cccgggtgtc gaggatggcg
     7081 cgagcttcac gcagcaggcg cgggcggtcg agggcagtgt ggtcggtcag caccagcacg
     7141 agatcataac cggcgaagtc ggcgttgggt ttttggctgt tcagctggag cgggggctga
     7201 tcgatttggg ggaccagcgg gtccatgtat tccacgtggg cgccgcgctg gatcaagccg
     7261 tggatgacgt ccaaagcagg agattcacgc acgtcgggca cgtcgcgttt gtaggccacg
     7321 ccgtagatga gtaagcgtga gccgcggacg gctttgcttt ggtcgttcag gacctccgcg
     7381 gcgcggctca cgacgtagtc gggcatggcg ttgttgatga cgtcggcgag ctcgatgaag
     7441 cgggcttgat gcttgtccgc acggaggcgc cacgagagat acagggggtc gaccgggatg
     7501 cagtgcccgc ccaggccggg gcccggatag aagggcatga atccgaaggg cttggtggcg
     7561 gcggcccgca cgacctcgaa ggcatcgatt ccgaatttgc ggctgatcat acagagctcg
     7621 ttgaccaagc cgatattcac ggcgcggaag gtgttttcca acagtttgac catctccgcg
     7681 gtttcggtcg aggacaccgg caccaggcga tcgatgaccg gcgaatagag cagctgagcc
     7741 atttcgaggc agttcgcggt tactccgcca atgactttgg gggtgttacg cgtgcggtaa
     7801 acgcggttac ccggatcgac ccgttcgggc gagaacgcga cgaagatgtc cgtgccgagt
     7861 tcataaccag cgcgggtgag acgtggcacg agcagctcag tggtcgtgcc cgggtacgag
     7921 gtggactcga gcaccacgag catgtcggag tgctggtggc aggcgatcag atcggccgca
     7981 gccacgatgt ggcgaatgtc tggatctcgg ctcttgttga gcggcgtggg tacgcaaatg
     8041 atgacggcgt ccccgcgcgc aatcacgctc ggatcgctgg tggcgctgag acgttggctc
     8101 tgtacgtggg gagtcaggtc ggcgttgatt acgtccggga cataggattc ccctcgacgg
     8161 acgctctcga ccttcttttc gtcgctgtcg aggcccgttg cccgataacc cgcccgcgca
     8221 aactcgacga gcagtggcaa gccgacgtat ccaatcccga tcacgacgac gtgggcggac
     8281 ttgtcgcgga ttttttgggc aagctcgggg cgcggcacgg cgcgcgaggg tagcaccgct
     8341 cgggaacgct aggattctcg cggttcttcc gtgggcgtgc tcggaccggg ttgcgcggcg
     8401 gcatcgacgc cctgcgcctc cgccgattgc gcctccgccg attgcgcctc cgcggagggc
     8461 gtgtctgctc gcggcggctc tgcaccggat gcagctgggg gctgcgcgct cggccgcgga
     8521 ccgcgcagac gggccaagcg ctcgcggatc gattgctcga gcccctgact cgagggttcg
     8581 tagaagcgct ggcctacgag ctgttctggg aggtaggttt ccccaggcac gtagccgccc
     8641 tcgaagtcgt gggcgtagcg ataacccgag ccgtagccct ctttgcgcat gagacccgta
     8701 accgcgttgc gcagcttcat cggcacgggc aacgcgccgt ggcgagcgat gcgctcgcga
     8761 acgccctgga tggcgcgttt gacggcgttc gatttgggac acaccgcc
//
LOCUS       DASTKU010000200        12283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1824059, whole genome shotgun sequence.
ACCESSION   DASTKU010000200 DASTKU010000000
VERSION     DASTKU010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12283
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824059"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..603
                     /locus_tag="VFQ61_07635"
     CDS             262..603
                     /locus_tag="VFQ61_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HET9954358.1"
                     /translation="MATERPKNPPEEEGELATRERVREERPRRYVVVLHNDDYTTMEF
                     VVHVLMKFFHLGETESVQLMLHVHHKGYAIVGTFTRDVAETKSAQVMAYAKEHGHPLK
                     CTAEPEGFGES"
     gene            600..2894
                     /gene="clpA"
                     /locus_tag="VFQ61_07640"
     CDS             600..2894
                     /gene="clpA"
                     /locus_tag="VFQ61_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HET9954359.1"
                     /translation="MKVSPEVEIACNLALREAERRRHDVMTVEHLLFALLHDEATAKV
                     AKKAGGNVDKLKARLDRILREDLPKVPDGQTITPSPSRGFQRVLQRAAFNVQSSGKER
                     LEGHNVLIAIFAEPESPAVEAMKAEGLTRYDVVNYVSHGVAKDGSDDDAMELSEGTEQ
                     REEEDEEKEDTRSDPLSQFAVNLNERARAGEIEPLVGREKELRRAIQVLCRRRKNNPM
                     FVGDAGVGKTAIVEGLAALIESGNAPAPLKNAVIYALDVGSLVAGTRFRGDFEKRVKG
                     ILKRLEKVPSAILFIDEIHTVIGAGAASGGTLDAANMLKPALSTGKLRCIGATTFEEF
                     RSHMERDRALSRRFQKIDVLEPSVAETVQILKGLRRRYEEFHEVTYTDEALDSSAELA
                     SRHLRDKKLPDKAVDLMDEAGADAKLDDGPGARVDVSRIEAVVARMAQIPPRQVSHDD
                     RSALRSLESDLQHAVFGQDRAIKELSSAIKLARAGLRPPDKPIGSYLFTGPTGVGKTE
                     AARQLALTLGIELIRFDMSEYMERHTVSRLIGAPPGYVGYDRGGLLTEAVSKTPHCVL
                     LLDEIEKAHPDVFNVLLQVMDHGTLTDNNGKKADFRHVIVIMTSNVGAQDLARAPLGF
                     GSRESKGRDDPAFRSTFSPEFRNRLDARIAFDPLTPAVMGRVVDKFIKELNKQLAERN
                     VTLNLSDPAREYLSEKGYDKDNGARPLARVIQDEVKRPLTEELLFGDLEFGGDVTIGF
                     RDGSLSFEIKGNPNPGAPPPKLLN"
     gene            2911..6468
                     /locus_tag="VFQ61_07645"
     CDS             2911..6468
                     /locus_tag="VFQ61_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET9954360.1"
                     /translation="MFVDRGGTFTDCVLYDRDTQRTSTLKVLSSDEAPLVAIRRLLSL
                     SPDAPIPPCDVRMGTTLATNALLERRGAPTLLVITRGFGDLIEIGTQTRPDLFALAIP
                     PKPRLYSEVLEVDARRSAQGGDLARPRAELVIPELRAARERGLTSAAISVLHDYRDGA
                     LELELGNWARQAGFEHVALGHEVCPELGFLNRTEMAVLDAYLTPPLAGYLEFLRRKLP
                     GSRLLVMQSGGALASPERCRGPHAILSGPAGGVVACAKIAESAGLPALIGFDMGGTST
                     DVCRVGDEFEQRPEQVVAGFRVRTPTLAVHTIAAGGGSLCRFDGHRLSVGPWSSGATP
                     GPLCYGHPAARELTLTDVNLLLGRLLPDAFPFELDAQRAHAGLRELTERVVAANHVRS
                     DLDVAEGLFEIANQSMADAISEVSTRRGYDVRQHSLLVFGGAGGQHACSLVRALGLRQ
                     AVFHPLAQVLSAYGIGLSPLGWQGARDVSAERVELTRLTDFEAAFAELETQGHASLQR
                     DGVESVSIERRVELGYAGTEARIPLRLEPVATLAERFHARHVAEFGYSRPDCAIVVQR
                     LRVELRGESHTALPMPGPDGSPSPPNEVLVYTRLYHHGRWHERVPVLQRGALQVGSHL
                     EGPLLVREANGTLVIDPGFSLECRADGLLVARPSAIANALDAVLQPSPDPALQVRSSL
                     DTADPVLLEILSHRFMSVAERMGHALQRSASSVNIRERLDYSCALFDSRGNLIANAPH
                     IPVHLGAMSESVRAVLTLHPEMEPGDAFVTNDPALGGSHLPDVTLVAPVHDAKGRLAF
                     VAARGHHADLGGITPGSMPAFSAHLHEEGVVFRAERAVHDGKLELEWLRERLNSGPFP
                     ARHPDQNLADLQAAFSALRTGAGLLLEACKDFGRDTVVRYMEFVQEHAADLVRRAIDE
                     LPADELAFEDALDDGTPICVRLVRHADSLEIDFSGTGAQVAGNLNAPRAVTLAGVIYF
                     LRTLVGKPLPLNGGFLRPVRVEIPVGSVLDPAPGAAVAGGNVETSQRVVDALLAAASA
                     AAASQGTMNNLSFGNASFAHYETICGGAGAGPTFDGASAVHTHMTNTRITDVEVLEQR
                     FPIRVREFQIRPGTGGTGRFSGGCGVRRVFEFLTPAEVSFLSERRTRAPFGLAGGGPG
                     ATGRQRLNGVDLPGKFSKHVEMGDVLTIETPGGGGYGAA"
     gene            complement(6493..7560)
                     /locus_tag="VFQ61_07650"
     CDS             complement(6493..7560)
                     /locus_tag="VFQ61_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954361.1"
                     /translation="MTTVRREGREPGLPSLNEARVQRRRAFVLGKVRMPGVPMHFWLW
                     TAVGIGAFGVIYWRISQGELESARGRLMAKQRAMSVALGPKLFPFRDKVEGFTRELAS
                     APSVVDFVSPNVDVERLRTQPSVYLRLRLERTKSVKELRKAVNASLRDGFTSCMFAST
                     KARPDPTQGAACRTAGDCVSGLLCNEWNVCAEAEQPYNVRLVYRTLRVLSSEWTDEVH
                     QATSELALAGYDRDLENVAKRDVPVTVQLVGRAKYFTLVLDEESKDGLPAEIPDAGES
                     HEERLQRVPHFARVGIWDLASGEPLVRLRTEAHGEFVPMGTRAVTNAANLAAQQRQVN
                     NCDLALRVRERLSGPAPSGTP"
     gene            complement(7727..8299)
                     /locus_tag="VFQ61_07655"
     CDS             complement(7727..8299)
                     /locus_tag="VFQ61_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9954362.1"
                     /translation="MSAEKALSRFHQPITVPGRRLLHHVPLAFVRKKVAGGGGRSVNQ
                     EIPLIPFIDFLLCIVLFLLASFSASGELPIDKNVKLPNAENVIDMTEAPMVAITGTQI
                     LVDGNAAGSTRAIEESNRMQRIDELFNVLKNKRELWKQLNPSREFPGVVILQVDRKVQ
                     ALVVKSVFQTAAFAGYPNVSFMVGRLGKNN"
     gene            complement(8338..8898)
                     /locus_tag="VFQ61_07660"
     CDS             complement(8338..8898)
                     /locus_tag="VFQ61_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9954363.1"
                     /translation="MAGIDVGGHGGKRSTNHEIPLIPFIDFLLCLVAFLLVTAVWSQM
                     ARLNADARVPGPPKDEPPEEIKKEKQLHVEMRGDQKFQLIWKEGNTVVNTIDVDKKQV
                     PVGAGGDYRYPDLAKKIADEWNQNGSHRAPSDTKWDQAVLHSDNSTPFSDIVAVIDAI
                     YTPKRDIPSRGKDSKVPAFNVTFSVQ"
     gene            complement(9011..9670)
                     /locus_tag="VFQ61_07665"
     CDS             complement(9011..9670)
                     /locus_tag="VFQ61_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9954364.1"
                     /translation="MASMWHHFQAGGWAMWIILFWLICSIAVIAERAVYLFGSSINKE
                     VFLATMQKCILAGDVAKAVKMCSAANAPLARIVQAGLVKVNRPDEEVQAAMDEAALRE
                     MPKINRRTGFLALFANLAMLSGLFGTIVGLIKAFGAVGGESVDPSQKARILAEGIAEA
                     MNCTAFGLLSAITALVGFAILNGKTQVLEDDINEASVQVLNLVVANRQKVSLQGLEQA
                     A"
     gene            11433..12113
                     /locus_tag="VFQ61_07670"
     CDS             11433..12113
                     /locus_tag="VFQ61_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9954365.1"
                     /translation="MQFLLLAAEGHSGSSQLVESFKQNPTFLVLNLCCSAVVLTIVIE
                     RFAFQLTQYRVNSKEFFAQVRKLVTAGNIDRAIKLCEASDFPILQLVKAGLTHANKGA
                     DEIDAALSEKLSELKPQAEKRVGALWSLANISTLIGLLGTVSGLILTFGAIASPALSQ
                     ADKQRMLSNGIAEAMYNTALGLGIAVFCMITHIVLHTRAKAIQHDLESTMERTFNLLT
                     ITQRPGSY"
BASE COUNT         2294 a         3883 c         3819 g         2287 t
ORIGIN      
        1 gctgcagtct agccagctgc gggagccccg agctttgctt tgcggccgat acccgccgtt
       61 cgcatccacg ccgtgtagat ccgcgaggcc cccgctcgcc gcactctccg cgaaacgcct
      121 cgtcccggac ggcctggtcc ccgaagccac caggggcgtg ctcggagcgc ggcagagatg
      181 cttagagtag gcggagattt gctgctaccc gtctgggggg ctcgacagcc agcacggaag
      241 tgcacaaagt tctggttgtc catggcgacg gagcgcccca agaacccacc cgaggaagaa
      301 ggcgagctcg ctacccgcga acgggttcga gaggagcgcc cgcgccgcta cgtcgtcgtc
      361 ctgcacaacg acgactacac gaccatggag ttcgtggtgc acgtgctcat gaagttcttc
      421 cacttggggg agaccgagag cgtgcagctg atgctgcatg tgcatcacaa aggttatgcg
      481 atcgtcggaa cgttcacgcg tgacgtcgct gaaaccaaat cagcccaggt catggcctac
      541 gcgaaagaac acgggcatcc gctgaagtgc accgcggagc cggaagggtt cggagagtcg
      601 tgaaggtcag tcccgaagtc gagattgctt gtaatttggc cctgcgcgaa gcagagcgcc
      661 gacgtcacga cgtgatgacg gtggagcacc tgctgttcgc cctattgcac gatgaggcca
      721 ccgccaaggt ggcgaagaag gcggggggca acgtcgacaa actgaaggcg cggctggacc
      781 gcatccttcg cgaagatctg ccgaaggtgc ccgacggcca aaccattacc ccttcgcctt
      841 cgcgcggttt tcagcgcgtg ctccagcggg cggctttcaa cgtgcagtcg agcggcaaag
      901 agcggctcga agggcacaac gtgctgatcg cgatcttcgc ggagccggag agccccgcgg
      961 tcgaggcgat gaaggcggaa gggctcacgc gttacgacgt ggtgaactac gtgtcgcacg
     1021 gggtcgccaa agacggctcc gatgatgacg caatggagct cagcgaaggt acggaacagc
     1081 gcgaggaaga ggacgaggaa aaggaggata cacgctccga tccgctctcc cagtttgccg
     1141 tgaacctgaa cgagcgggcg cgcgcaggtg agatcgaacc gttggttggg cgggagaagg
     1201 agttgcgacg agcgattcag gtgctctgcc gccgccgcaa gaacaacccg atgttcgtgg
     1261 gtgacgcagg agtcggaaag acggccatcg tcgaggggtt ggccgcgttg atcgagagcg
     1321 gcaatgcacc cgcccccctc aagaacgcgg tcatttacgc gctggatgtc ggctccctcg
     1381 tcgccggcac tcgcttccgc ggtgacttcg agaagcgtgt gaaagggatc ctgaagcgcc
     1441 tcgaaaaagt gccgtcggcg atcctcttca tcgacgagat tcatactgtg attggcgcgg
     1501 gagccgcgag cggcggcacg ctcgacgccg cgaatatgct caagcccgcg ctatccacgg
     1561 ggaaattgcg ctgcatcggg gcgacgacgt tcgaggagtt ccgcagccat atggagcggg
     1621 atcgcgccct ctcgcgccgc ttccaaaaaa ttgacgtgct cgagccgagc gtcgccgaaa
     1681 ccgtgcaaat cctgaaaggg ctgcgccgcc gctacgagga attccacgag gtcacgtaca
     1741 ccgacgaagc tctcgacagc tccgccgagc tcgcgagtcg acatttgcgg gacaagaagc
     1801 tgccggacaa ggccgttgac ttgatggacg aagctggcgc agacgccaag ctcgacgacg
     1861 gacctggcgc gcgggtggac gtctctcgca tcgaggcggt ggtggctcga atggcccaga
     1921 ttcccccgcg tcaggtcagc cacgacgatc gctccgcact gcgcagccta gagagcgacc
     1981 tgcaacacgc ggtgttcggg caggatcgcg ccatcaaaga gctctcctcc gcgatcaagt
     2041 tggcgcgagc aggtctgcgc ccgccagaca agccgattgg ctcgtacttg ttcacgggtc
     2101 ctacgggtgt tggtaaaacc gaggccgcac gccagctcgc gctcacgctg ggtatcgagc
     2161 tcattcgctt cgatatgagt gagtacatgg agcgccacac ggtttctcga ctgatcggcg
     2221 cgcctcccgg ctacgtcggg tatgatcgag gcgggctgct cacggaggcc gtttcgaaga
     2281 ccccgcactg cgtgctgctg ctcgatgaga tcgagaaggc gcaccccgac gtcttcaacg
     2341 tgctgcttca ggtgatggat cacggcacgc tgaccgacaa caacggcaag aaagccgatt
     2401 tcaggcacgt catcgtaatc atgacgagca atgtcggcgc ccaagacctg gcgcgcgctc
     2461 ccctcggttt tggcagtcga gagagcaagg gccgtgacga tccagccttc cgaagcacct
     2521 tcagccccga gttcagaaac cgtttggatg cacgtattgc gttcgatcca ctcacgcccg
     2581 cggtcatggg gcgggtcgtg gacaagttca tcaaggagct caacaagcag ctcgcagagc
     2641 gcaatgtaac gctcaacttg tccgatccgg cgcgcgagta cctgtccgag aaggggtacg
     2701 acaaggacaa cggggcacgg cccctcgcga gggtgatcca agacgaggtc aagcgtccct
     2761 tgaccgaaga gctgctgttc ggagatctag aattcggcgg ggacgtgacg attgggttcc
     2821 gcgacgggtc gttgagcttc gagatcaaag gcaatcccaa cccgggcgcg ccgccgccca
     2881 agctcctgaa ctagctcttt gcggtaccgt gtgttcgtcg atcgcggcgg tacgtttacc
     2941 gactgcgttc tgtatgaccg tgacacgcag cgcacctcca cgctgaaggt cctgtcgtcg
     3001 gatgaggcgc cactcgtggc gatccggcgg ctgctctcgt tgtcgccaga tgcgccgatc
     3061 ccgccctgcg acgtgcgcat gggcacgacg ttggcgacga atgcattgct ggagcggcgc
     3121 ggagcaccga cgttgctcgt aatcacgcgc ggctttggtg atttgatcga gatcggaacc
     3181 cagacgcgac cggacttgtt cgcgctcgca attccgccaa agccccgctt gtactccgag
     3241 gtgctggagg tggacgcgcg gcggagcgcg cagggtggag acctcgcgcg gccccgcgcc
     3301 gagctcgtga ttcccgagct tcgcgcggcg cgtgaacgag ggctcaccag cgccgcgatc
     3361 tcggtgctgc acgactatcg agacggagct ctcgagctcg agctcggcaa ttgggcgcgg
     3421 caggccggct tcgagcacgt ggcattgggg cacgaagtct gccccgagct cggcttcctg
     3481 aatcgcacgg aaatggccgt gctcgacgct tatctcacac cgccgcttgc aggctacctc
     3541 gagttcttgc ggcgaaaatt gccgggtagt cgcctcttgg tgatgcaatc gggtggcgcc
     3601 ttggcatcac ccgagcgctg tcgcggccca catgccatcc tctcggggcc ggccggcggt
     3661 gtggtagcct gcgcgaaaat cgcggagagc gccgggcttc cggcgctgat cggcttcgat
     3721 atgggcggca cgtcgaccga cgtttgccgc gtcggtgacg agttcgagca gcgcccggaa
     3781 caggtcgtcg ccggatttcg ggtacgcacc cctaccctcg ctgtgcatac gattgcggcc
     3841 ggcggtgggt cactgtgccg cttcgatggg catcgactga gtgtcggccc atggagctcc
     3901 ggcgccacac ccggaccact ctgttacggc caccccgcgg cgcgagaact gacgctcacc
     3961 gacgtgaatc ttttactagg acggctgctg cccgacgcct ttcctttcga gctcgatgct
     4021 cagcgagcgc acgcgggtct tcgcgagctc accgaacgcg tcgtagcggc caatcacgtg
     4081 cgctcagatc tcgacgtggc ggagggtttg ttcgagatcg ccaaccagag catggcggac
     4141 gcaatctccg aggtgtccac gcgacgcggc tacgacgtga ggcaacactc gctgctcgtg
     4201 ttcgggggag cgggcggtca gcatgcgtgc tcactcgtcc gcgcgttggg actacgccag
     4261 gctgtttttc atccgctcgc gcaggtgcta tcggcctatg gcattggact ctcgccactc
     4321 ggctggcagg gggcacgcga tgtgtccgcg gaacgcgtcg agctgactcg actcactgac
     4381 ttcgaagcgg cgtttgccga gttggagacc caaggccacg cgagcttgca gcgcgacggc
     4441 gtcgaatccg tgtcgatcga acgtcgggtg gagctcggct atgctgggac cgaggcacgc
     4501 atccccctac gcttagagcc cgtagcgacg ctcgccgagc gatttcacgc gcgccacgtc
     4561 gccgagtttg gctattcgcg cccagattgc gccatcgtgg tgcaacgcct gcgcgtagag
     4621 ctccgaggcg aatcgcacac ggccctgccc atgcccggcc cagacggctc cccttcgccg
     4681 cccaatgaag tacttgttta cacgaggctt taccaccatg gccgctggca cgaacgagtg
     4741 cccgtcttgc aacgcggcgc actgcaagtg ggctcgcatc tggaggggcc gttgctcgtg
     4801 cgcgaagcca acggcacgct ggtgatcgat cctggcttca gcctcgagtg tcgagccgat
     4861 ggtttgctgg tggctcgccc gagtgccatt gcgaacgcgc tcgacgctgt cctccagccc
     4921 agtcctgatc cggcactgca ggttcgctcg agcctcgaca cggcggaccc ggttttgttg
     4981 gagatcttga gccaccgctt catgtccgtg gctgaacgaa tgggtcacgc gctgcaacgc
     5041 agcgcttcga gcgtgaacat tcgggagcgg ctcgactact cgtgcgcgct gttcgactca
     5101 cgcggcaacc tgatcgccaa cgctccccat attcccgtgc atctcggtgc gatgagcgaa
     5161 tcggtgcggg ccgtactgac gttgcacccc gagatggaac ctggcgatgc gttcgtgacc
     5221 aacgaccccg cgttgggcgg gtcacacctg cccgatgtca ctctcgtcgc ccccgtgcac
     5281 gatgcgaaag ggaggctcgc gttcgtggcc gcgcgcgggc atcacgcaga tctcggcggg
     5341 atcaccccgg gttccatgcc cgcgttctct gcacacttgc atgaagaggg ggtggtcttc
     5401 cgagccgagc gcgccgtgca cgatggcaaa ctcgagctgg aatggctgcg cgagcgcttg
     5461 aactcgggcc cgttccccgc tcgtcacccg gatcagaatt tggcggactt gcaggccgca
     5521 ttcagcgcgt tgcgcactgg cgctgggttg ctgctcgaag cttgcaagga ttttggtcgt
     5581 gacaccgtgg ttcgctacat ggaattcgtt caagagcatg ccgcggacct cgttcgacga
     5641 gcgatcgatg agctgcccgc cgatgagctc gccttcgaag atgcgttgga cgacggaacg
     5701 ccgatttgcg tacgcctcgt ccgtcacgct gactcgctcg agatcgattt ttccggcacc
     5761 ggtgcccagg tagctggcaa tctcaacgca ccgcgcgcgg taacactcgc gggggtgatc
     5821 tacttcctac gcactttggt cggcaaaccg ctgcccttga acggtggttt tctaagaccc
     5881 gtgcgcgtcg agattccggt tgggagcgtg ttggatcctg cgccgggcgc ggcggtcgcg
     5941 ggaggcaatg tcgaaacttc tcagcgggtc gtagacgcat tgctcgccgc cgcttccgcg
     6001 gcggcggcga gccaaggcac catgaacaat ctctcgttcg gcaacgcgag cttcgcgcac
     6061 tacgagacta tttgcggggg agcgggcgcg ggtccgacct tcgatggcgc gtcggcggtc
     6121 cacacgcaca tgaccaacac gcgcatcacg gatgtcgaag ttctggagca gcgtttcccg
     6181 atccgcgtcc gcgaattcca gattcgtcct ggcaccggag gcaccgggcg cttttcaggc
     6241 gggtgtggcg tgagacgcgt gttcgagttc ctgacgccgg cggaggtttc cttcctgagc
     6301 gagcgccgca ctcgggcgcc gttcggcttg gccggtggtg gccctggcgc gacgggccgt
     6361 cagcggttga atggcgtcga cttaccggga aaattcagca agcatgtcga gatgggcgac
     6421 gtgctaacga tcgagacgcc gggcggcggc ggctacggag ccgcctaaaa accgagctcc
     6481 gaccagattc aactacgggg tgccgctcgg cgctgggccc gacaaccgct cgcgaacgcg
     6541 cagcgccaga tcgcagttat tcacctgccg ttgctgggcc gccaagttgg ctgcgttggt
     6601 aaccgctcga gtgcccatcg ggacgaactc gccatgagct tcggtgcgca aacgcaccag
     6661 aggttccccg cttgctagat cccagatccc gacccgcgcg aagtgaggca ctcgctgcag
     6721 gcgctcctcg tgcgactccc ctgcgtccgg aatctcggcg ggcaaaccgt ccttcgattc
     6781 ctcgtcgagc acgagcgtga agtacttggc tcgcccaacc agctgcacag tgaccggcac
     6841 atcccgtttg gcgacgttct ccagatctcg gtcgtagccg gcgagcgcca gctcactcgt
     6901 ggcctggtgc acctcgtccg tccactccga agacaggact ctcagggttc gatagacgag
     6961 ccgcacgttg taaggctgct ccgcctccgc gcagacattc cactcgttgc agagcaaacc
     7021 ggacacacag tcccccgcag ttcggcacgc agcgccctgc gttggatccg ggcgagcctt
     7081 ggtcgaggcg aacatgcacg acgtgaagcc atcccggagc gaggcgttca ctgctttgcg
     7141 cagctccttc acgctcttcg tccgctcgag gcgcagccgc aagtagaccg acggctgcgt
     7201 gcgcagccgt tcgacatcga cgttcgggct gacgaaatcc accacgctcg gcgccgacgc
     7261 caactcacgc gtaaacccct cgaccttgtc ccgaaaagga aacaacttcg gccccagcgc
     7321 caccgacatc gcccgctgct ttgccatcaa tcggccgcgc gcgctctcga gctccccttg
     7381 agagatgcgc caatagatca ctccgaacgc gccaatgccc accgccgtcc aaagccagaa
     7441 gtgcatgggc acccccggca tgcgcacctt ccccaacacg aacgcccgac ggcgctggac
     7501 gcgcgcctcg ttcaaagaag gcagacctgg ttcccggcct tctctccgca ctgtcgtcac
     7561 tcgaacctct tcagagtcag cttgaaattg tcgccttgcg tccgaccggc tccccagaaa
     7621 cagctacgcc ggccttgcat tccgctcggc cggcgcgctt tccgctttcg gagctcaaca
     7681 cccctgaatc atcaggagct tcgagctcgg ggagcatgag caactctcag ttgttcttgc
     7741 ctagacgtcc aaccatgaag ctcacgttcg gataccccgc gaatgccgcg gtctggaaca
     7801 cgctcttcac gaccagcgcc tgcactttgc gatcgacctg gagaatgacc acacccggga
     7861 actctcggct cgggttcagc tgcttccaga gctcacgctt gttcttgagc acattgaaga
     7921 gctcatcgat gcgctgcatg cgattggatt cttcgatggc gcgggtgcta ccggcggcat
     7981 ttccatcgac cagaatttgg gttccggtga tggccaccat cggcgcctcg gtcatatcga
     8041 tcacgttctc ggcgttcggc aacttgacgt tcttgtcgat cggcagctcg ccactagccg
     8101 agaagctcgc cagcaagaac aacacgatgc agaggaggaa gtcgatgaac gggatgagcg
     8161 ggatctcttg gttcaccgag cgaccgccgc cgcccgctac cttcttccgg acgaacgcga
     8221 gcggaacatg gtgcagcaaa cgccgtcccg ggacggtaat cggctggtga aaacgactca
     8281 gggctttttc ggcagacatc tgcagttcct tattggactc agcctaatta cctgggccta
     8341 ctggaccgag aacgtcacgt tgaaggccgg caccttgctg tcttttccac gcgaggggat
     8401 atcccgcttc ggcgtgtaga ttgcgtcgat gacagcaacg atgtcgctaa aaggcgtcga
     8461 gttgtcggag tggagcaccg cctgatccca cttggtgtca gagggcgcgc ggtgagagcc
     8521 gttttggttc cactcgtcgg cgatcttctt cgcgagatcc ggatagcggt agtcgccacc
     8581 agctcccacg ggcacctgct tcttgtcgac gtcgatggtg ttgacgaccg tattgccttc
     8641 tttccagatg agctggaact tctggtcgcc acgcatctcg acgtgcagct gcttttcctt
     8701 cttgatctcc tcgggaggct catccttcgg agggcctggc acccgcgcgt cggcgttcag
     8761 gcgggccatc tgcgaccaaa ccgccgtcac cagcaggaag gccacgaggc acagcaaaaa
     8821 gtcgatgaac ggaatcaggg gaatctcgtg gttggtggag cgcttgccac catgtccacc
     8881 gacgtcaata ccagccattt cttactccgc tgctcgtctg ggaagctcta gaactggaat
     8941 ggtgggcctt cgctccaaca acaccgaggt tccttcgatg aagctcgggc ccaccgctcg
     9001 ttctcgcgta tcaggcagcc tgctcgagac cctggaggct caccttctgg cggttggcaa
     9061 ccaccagatt gagcacctgc acagaagcct cgttgatgtc gtcctcgagg acctgggtct
     9121 tgccattgag aatggcgaag ccgaccaggg ccgtgatggc agagagcaaa ccgaaggccg
     9181 tgcagttcat ggcttcggca ataccctctg cgaggatgcg ggccttttgg ctcggatcga
     9241 ccgactcacc gccgaccgcg ccgaatgcct tgatgagacc cacgatcgtt ccgaacagac
     9301 cgctcaacat cgcgaggtta gcgaacagag cgagaaaccc cgtacgacgg ttaatcttgg
     9361 gcatttcgcg caaggccgct tcgtccattg ccgcctgaac ttcctcatcc gggcgattca
     9421 ccttgacgag accagcctgg acgatgcgcg ccagcggagc gttggccgcc gaacacatct
     9481 tcacggcttt cgccacgtca ccagccagga tgcacttctg catagtggca aggaagactt
     9541 ccttgttgat ggatgaaccg aacaaataga cagcgcgctc agcaattacg gcgatggagc
     9601 agatcaacca gaacaggatg atccacattg cccaaccgcc cgcctggaag tgatgccaca
     9661 tactcgccat tctcgtttac tccggctagt gctcctgatg gacacccgcc cggcgcccca
     9721 cgcttggggt gcgaacacgg tgtctaaata tctatcggtg cctgatcgac cgcggccgac
     9781 gcctcggacg cctcttccgc tcgtcattct gcggattcgg accggggcat tctgttcaag
     9841 ttgaacccgg gtcgtcaaga gagattggat ttcaggctgc tcttcgtttt gagccacaaa
     9901 gttccgcttg agctggcggg tttcgccggc ttttctgcgt tctggagcgc gctcagggtc
     9961 ggatgattga ggtcagtttc ggatagtgag gctgctttcc cgcgcagccg gcctgttcga
    10021 aactgagcgc atccggccat ttggctgcgc acttttgcaa aagcgaaccg cccgccgacg
    10081 ggaacccggt gcggccgtgg gcaagcgcgt cgatcgagcg gggactttcg agggaaccgg
    10141 ggggaccgtg aagaacgtct gagagccggg cgggaccaag agcttaggcg gagcgttgga
    10201 cagggacatg gtcgtccaaa ggagcaaccg agtcgctggg atctagtaac ccggtcctcg
    10261 ccgcatccgt cggtgaggcc atgcaccggc gcgagaatca cgccgaatct tcattcgacc
    10321 cacggggcgt tcggtgctcc acgcggtgct ctggaagccg cgtctttact ttcgcgcagg
    10381 gcgggcgctg aacttcgcgt tcgaggcgcc gtaagcagct cactaggaac ccgaagccag
    10441 cttgaactaa gtggcagacc aggtgggccg accacattgg gcatgctcgc agctgacgta
    10501 ggacctgttt ggaggacttc gcacttggag cgcttgcttg tgcgggtgag ctttgcgacg
    10561 gaatcgcttc cgcgcttggc cgggctcggc tcgatcgagc tgaattggtt cggacatgca
    10621 ttgttcggga gcggctcttc gcccgcgccg aaccggttcg gactttgcgg gcccgctccg
    10681 ttagcgaatc acttctataa caccacgcgc accggaagct gcggccgcgc atgttcgcgt
    10741 gcccccggcg cgatgggctt gccgctcccc tcgcgggtca atcagcgcac tcgacgtggc
    10801 acgacgacac gccacgcctc cccagtgaat cgaaagtctg ccgcgctacc ggttccaata
    10861 gaccgcccgc tttcgctctc gcaaagctcg ccgccacagg gaacatcagc gcgcgggcgg
    10921 cctgcgcctg gagcggtggc atctgaagtt ggtgaagcta gtgtgccgag agacccagtg
    10981 ccgggacaca cagtgccgag acacacagtg ccgagacaca cagcgtggtc ccacgcaggc
    11041 tgcatgcagt tcgcagtggt cgatcagatc ttgctgatcc cggcagcatc ccggccgctt
    11101 cgcgtggctt tggcgcgaat tgctctgcag attgcgcgtt tccgtcagcg aacagcgcaa
    11161 atacggccaa cgcaaccgca tggagctcag cgacgcagcg accaaagcac ggagcggcag
    11221 atcagccgcg ttgtgcgtgt gaatcagaca ctcaacaaga ccggccagca gggcgaaaaa
    11281 ccgagtaatg agggatgctt gcgctcccaa gttggcgacg gctatagtgc gccctcggcc
    11341 tcaactgggc cctccatcgc gcggctccaa caccgacgtt ccagtaactg gaagcccgaa
    11401 ttagtcaggt ccaaagaagg gaagaagggg taatgcagtt cctgctcctt gcagccgaag
    11461 gtcacagcgg atccagccag ctcgtcgaat cgttcaagca gaatccgaca ttcctggtcc
    11521 tgaacctctg ctgttcggcg gtggttttga cgatcgtcat cgagcgcttc gcgtttcagc
    11581 tcacgcaata ccgcgtcaac tcgaaggagt ttttcgcgca ggtgcgcaag ctggtcaccg
    11641 cgggcaacat cgaccgggcg atcaagctgt gtgaagccag cgatttcccg attctgcagc
    11701 tcgtcaaagc tggcctcacc cacgccaaca aaggcgcgga cgagatcgac gccgcactca
    11761 gcgagaagct ctcggagctc aaaccacaag ccgaaaagcg cgtgggggct ctatggtcgc
    11821 tcgccaatat ctctacgttg attgggctgc tcggtacggt gagcggtctg atcttgacgt
    11881 tcggagcgat cgcgtcccct gccctgagtc aggcggacaa acagcgcatg ctatccaacg
    11941 gtatcgccga agccatgtac aacacggcgc tcgggctcgg catcgccgtg ttctgcatga
    12001 tcacgcacat cgtgctccac acgcgcgcca aagccatcca gcacgacctc gagtcgacga
    12061 tggagcgcac cttcaacctg ctgaccatta cccagcgacc tggctcgtac tgagggcgtt
    12121 ttcgcccgct aggccttcgt tgcgaatcgt ccactcggca gggagcgcgt catgacactc
    12181 agcgcagctc agcgcagtaa gattcgtcgg ctcagtcagc cgcgcgaatt gtcgcccgac
    12241 gaagagggtg gcgagctcaa tatcgttccg ttcctcgata ttg
//
LOCUS       DASTKU010000201         4039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_486120, whole genome shotgun sequence.
ACCESSION   DASTKU010000201 DASTKU010000000
VERSION     DASTKU010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486120"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VFQ61_07675"
     CDS             <1..1354
                     /locus_tag="VFQ61_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET9954366.1"
                     /translation="QELVEWAEPGLASMASPRFFGWVTGGSLPAAVAADWLTSAWDQN
                     AGPSSATPSSCAFESCALSWVVQLLELPPDTRGAFVSGCSMANLTALAGARSQVLRSA
                     GWDVEREGLFGAPAVRVFVGQERHESIDRALRLLGFGKRSIRFLATDAQGRLRSDALR
                     AALLRGAEKSPVIVCAQLGNVNSGACDPLPELHEILEEYRTTCSPEHAWLHVDGAFGL
                     WLRASPSLRALAAGAELADSWATDAHKVLQVPYDSGIIFTRHVRAQRRAMAIQGAYLS
                     SGHEPQAPNGGALIPDLSRRARGFVTWAALRSLGRRGVVEWIDRAAALAQHFASSLHA
                     VEGLRILNEVTFNQVVVEATAPALARGSTVEARRDFTARWARAIQAEGTTFATPTLFQ
                     GRPALRLSVMNADSTERDIERSARAIRDVYRRLVADDQNRTQSEGAGARNESSRSSLE
                     "
     gene            1373..2008
                     /locus_tag="VFQ61_07680"
     CDS             1373..2008
                     /locus_tag="VFQ61_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954367.1"
                     /translation="MQRLLIYDATQHTRAPRSLGLAWQFGSYLYRGLGRIDAAYGARR
                     FDAALRWVRRRADEHGPVTELQYWGHGKWGAVFVDRERLDRGALAPAHPLRRDLDAIR
                     ECLAPNALIWFRTCETLGANAGQDFASALGDYLGARIAGHTFVIGFYQSGLHELPPGC
                     RADWSASEGLAVGSPGAPQRALNSAPSQPNTITCLEGRVPTQWATQQLPVR"
     gene            complement(2023..2538)
                     /locus_tag="VFQ61_07685"
     CDS             complement(2023..2538)
                     /locus_tag="VFQ61_07685"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9954368.1"
                     /translation="MFDRRELSLRPVVESDQAFLFELYQSTRSVEARALGLDGHALEA
                     FMRLQFSARNRGYRAAFPHAEQSIVMLAGQTIGMLWVDRQGGSIRLVDIALGRAFRGS
                     GIGTSLLGDLQREASQRGCPLYLHVVKSSRAARFYERLGFVAVDPDVAGPHIELNWQP
                     ETFTSSPLSPD"
     gene            complement(2596..3114)
                     /locus_tag="VFQ61_07690"
     CDS             complement(2596..3114)
                     /locus_tag="VFQ61_07690"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HET9954369.1"
                     /translation="MEPFIGEIRIFTGNFAPQGWALCNGQLLPISQNQALFAILGNTY
                     GGDGRQTFGLPDLRGRVAIHAGQGAGLSAYNLGQTGGNETVTLESTQLPPHTHAFAPA
                     CSSSTPDAVDPANAVPAAVESMQLYSGSANASMQAANTTAAGGGQAHNNVQPYLAVNF
                     IIALQGIFPSRG"
     gene            complement(3359..>4039)
                     /locus_tag="VFQ61_07695"
     CDS             complement(3359..>4039)
                     /locus_tag="VFQ61_07695"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET9954370.1"
                     /translation="DGGARTLELQVIEPIRNPLEMDTTLESVRARLATDPELVALFEQ
                     AYGEGPSEFTIPRALASFVRSLLSGQSAWDRYRRGEHLALSDSAARGLELFNGERAEC
                     FHCHVGFNFTNNSFRNNGGRADDPDVGREKLTGKASDRGKFKVPSLRNVAVTAPYMHD
                     GSLATLDAVIDRYVAGGLGHPNTDPVVFPLDLTDSEKADLKAFLESLTDPEFLSEPSF
                     ADPASAAL"
BASE COUNT          754 a         1277 c         1273 g          735 t
ORIGIN      
        1 gcaggaactc gtcgaatggg cggaacccgg gctcgcgagc atggcgagcc cgcgcttctt
       61 tggctgggtg accggcggct cgctgcccgc agcggtcgct gccgattggc tcacgtccgc
      121 ttgggatcag aacgccggtc ccagcagcgc aacgccttca tcttgcgcat tcgagagctg
      181 tgcactgagc tgggtcgttc agctcctaga gctgcccccg gacacgcgag gcgccttcgt
      241 gagcgggtgc tcgatggcca atttaacggc cctcgccggc gcgcgtagcc aagttctacg
      301 gtcggcgggc tgggacgtgg aacgggaagg cctattcggc gctccggcgg tccgagtctt
      361 cgtgggtcag gagaggcacg agagcatcga tcgcgctctg cggctgctcg gcttcggcaa
      421 gcgctcgatc cgttttcttg cgaccgacgc tcaaggccga ttgcgcagtg acgcgctccg
      481 ggccgccctg ctcaggggcg ccgagaagtc acccgtgatc gtgtgcgctc aactcgggaa
      541 cgtgaatagc ggagcttgcg atcccttacc ggagttgcac gagattctcg aagagtaccg
      601 gacgacgtgc agcccagagc atgcgtggct tcatgtggac ggcgcgtttg ggttgtggct
      661 gcgagccagt ccaagtctac gcgctctcgc ggcgggagcc gaactagcgg actcctgggc
      721 cacggacgcc cacaaggtgc tccaagtccc ctatgattcg ggcatcatct tcacgcggca
      781 cgtgcgggcc caacggcgag ccatggccat tcaaggcgca tacctgagct cggggcacga
      841 accgcaagca cccaacggcg gagcgctgat cccggacctt tcgcggcgtg cgagaggctt
      901 cgtcacgtgg gcggcgctgc gctcacttgg ccgccgcggc gtggtggaat ggattgaccg
      961 ggccgcagcc ttggcgcaac acttcgcgag ctcgttgcac gccgtcgagg gcctacgcat
     1021 cctgaacgag gtgacgttca atcaagtcgt cgtcgaagcc acggcgcccg cgttggcgag
     1081 gggatcgaca gtcgaggcgc gccgcgattt tactgcccgt tgggcgcgcg cgattcaagc
     1141 cgaaggaacg acattcgcga cccccaccct gtttcaaggt cgcccggcgc tgcggctttc
     1201 ggtgatgaac gcagacagca ccgaacggga tatagagcgc agtgcgcggg ccattcgcga
     1261 tgtttatcgg cggctcgtgg cggacgatca gaaccgcacg caaagtgaag gggctggcgc
     1321 gcgcaacgag agctcccgca gctcattgga atagcccgag acacggagcc cgatgcaacg
     1381 cttgctcatt tacgatgcca cccagcacac gcgcgctcca cgtagcctcg gtctagcctg
     1441 gcagttcggt agctatctct atcgaggact gggcagaatc gatgcagcct atggggctcg
     1501 acgtttcgac gctgcgctgc gctgggttcg aaggcgcgcg gacgagcacg gccccgtcac
     1561 cgaactccag tattggggtc acggcaaatg gggtgccgtc ttcgtcgacc gagagcgctt
     1621 ggatcgtggc gcgttggccc ccgctcatcc tctgagacgt gacctggatg cgatacggga
     1681 gtgtctggcg ccaaacgcac tcatctggtt tcgcacctgc gagaccctcg gcgcaaacgc
     1741 tgggcaagat ttcgcgagcg cgctgggtga ctacttgggc gcgcgcatcg ccggtcacac
     1801 cttcgtgatc ggcttctacc aaagcgggtt gcatgaactg cctcccggtt gtcgggcaga
     1861 ctggtccgcg agcgaaggat tagctgtagg cagcccgggc gcgcctcagc gagccctgaa
     1921 ttcggcgccc agccagccca acaccatcac ttgcctcgag gggcgggttc ctacgcaatg
     1981 ggcaactcaa cagctgccgg tgcggtgatc atctggtgat gactaatccg gcgatagcgg
     2041 agacgatgtg aaggtttcgg gttgccaatt cagttctatg tgcgggcccg cgacatcggg
     2101 atcgaccgcg acgaagccga gccgctcgta aaaacgcgcg gcccggctcg atttcaccac
     2161 gtgcaaatag agaggacagc cccgttgact cgcttcccgc tgcaagtcac ccagcagcga
     2221 cgttccgatt cctgaaccgc gaaacgcgcg gcccaacgca atgtccacca gacgaatgct
     2281 gcccccctgc cggtcgaccc acagcatgcc gatcgtttgc cccgccagca tcacgatgga
     2341 ctgctccgcg tgcggaaaag cggctcgata accgcgattg cgcgcggaga actgcagcct
     2401 catgaatgcc tctagcgcgt gcccgtcgag cccgagcgcc cgagcctcga cgctcctggt
     2461 actctgatat agctcgaaca agaacgcctg atcgctctct acgaccggcc gcaacgacag
     2521 ctctcgtcga tcgaacatgg tttgccttag gacggggttt ggccccagaa cagcttggcc
     2581 tcaggacagg atcgctcagc cacgactcgg gaaaatgcct tgcagcgcga tgatgaagtt
     2641 cacggccaaa tagggctgca cgttattgtg ggcttgcccg ccaccagctg ccgtcgtgtt
     2701 cgcggcttgc atgctcgcgt tggcactgcc ggaatatagt tgcatcgact cgaccgcggc
     2761 cggcaccgcg ttggctggat ccacggcatc gggtgtggac gaggagcagg caggcgcgaa
     2821 cgcgtgagta tgcggcggca gctgggtgct ctccagcgtg accgtctcgt taccgcctgt
     2881 ttggcccaag ttgtacgcgc tgaggcccgc gccttggccc gcatggatcg ccacccgccc
     2941 gcgcagatcg ggcaacccga aggtttggcg cccgtcccca ccgtaggtgt ttccaagaat
     3001 cgcgaacaag gcttggttct gtgaaatagg taacagctgc ccgttacaca acgcccaacc
     3061 ctggggcgcg aaattgccgg tgaagatgcg gatctcgccg atgaatggtt ccatatcgcc
     3121 tcctcgtcat ttgcgtgctt cgaccgggcg gcagcaaccc gcgaccggcc cgagcttgct
     3181 tccgatgccc tcaaacggcg tcgatagatt ggacctaata ggtgggaatc accggttcgg
     3241 aattttgagg tcgaatgcga tctgttttta gaacgcggag cagaggcgct acacgggcta
     3301 cgacggttgc cgtgacgaga tccgcgccgc cgcctgccac acgagctagc gcaacgactc
     3361 agagcgcggc ggaagcggga tccgcgaagc tcggctctga aagaaattcc ggatcagtca
     3421 gactctcgag gaaggccttc aaatcggcct tttcggagtc ggtcaagtcc aatggaaaca
     3481 ccacgggatc cgtgtttgga tgaccgagtc cacccgcgac atagcgatcg atcaccgcgt
     3541 ccagcgtggc tagtgaacca tcgtgcatgt agggcgcagt gacagccacg ttgcgcaacg
     3601 aaggcacctt gaatttccct cgatcggacg ccttaccagt cagcttctcc cgcccgacgt
     3661 cgggatcgtc ggcgcgaccg ccgttgttgc ggaaagaatt gttcgtgaag ttgaagccga
     3721 cgtgacaatg gaagcactca gcgcgctcac cattgaacag ctcgagccct ctggctgctg
     3781 agtcgctcaa ggccaagtgc tcgccgcgcc tgtaacggtc ccacgcactt tgcccgctca
     3841 acaagctgcg cacgaaactg gccaacgcac ggggtatggt gaattcggac ggtccttcgc
     3901 cataggcctg ttcgaacaac gccaccaact ccggatcggt cgccagtcgc gcccgcaccg
     3961 attcgagcgt ggtgtccatc tccaaaggat tgcgaatcgg ctcaattacc tggagctcga
     4021 gggttcgcgc gcctccatc
//
LOCUS       DASTKU010000202         2728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_40028,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000202 DASTKU010000000
VERSION     DASTKU010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40028"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..965)
                     /locus_tag="VFQ61_07700"
     CDS             complement(96..965)
                     /locus_tag="VFQ61_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954371.1"
                     /translation="MDTVTLTRDELYSLVWSRPMTAVAEEFGVSSVAFAKYCVKLGIP
                     RPSRGYWQQLAAGFKPRPDKLPKPGPRTPTTITLTRHRKSERTVRDRPRPPDVSVGDG
                     LSRPHPVVKQIRGQMRPLRPDEHGMSGVRGDGHAVFKVGKETERRGLLLLDALFKALT
                     ARGHQVELRNRSEVGRGRCALLVTINREPVEFWLVEHADRSAHVPTAKELEDQAKSGW
                     RTPQKYDFTASGRLAFEATWTQAEGLRRRWSDGPKQRLEHLLGDVVVGLEAIAAALTG
                     IRCTEPGLEGVAS"
     gene            1051..1449
                     /locus_tag="VFQ61_07705"
     CDS             1051..1449
                     /locus_tag="VFQ61_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954372.1"
                     /translation="MKLRVADLREIVRAVVADALATHAQHDLLDLKQVFERYRVGRDA
                     LVAAAKRGEVELSQGPRRKFLIRARELEQWLTRRKYVPPALATAKDLQEWDRQADLAL
                     ERALVQGRLRQLSPPELEEARARRSRKKRP"
     gene            complement(1490..2497)
                     /locus_tag="VFQ61_07710"
     CDS             complement(1490..2497)
                     /locus_tag="VFQ61_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954373.1"
                     /translation="MLAAERQTVHVPAPNEPGYDIVLTLPSGKRLRVSCKALGSSAYD
                     AALKADARRAFEVLRQQLPSGCAQHLVLEAITAKAPLPPAEQLLSLARGCSVVTTSAC
                     LWMRDGSWRIAVVPLGRLNNAEFSDEKVSVGLSVFVPLHVDEQKRFDDKIDDACTNLA
                     RHFSSATADEGSVIAMSVPEEVSMQQARAYALSSRLGRHPSVAAALLFRTQVCSNGKD
                     ISLGHEFEVVQNPAAQFALAHYAPSGSLTVQMLLGNVLGAVPVETPTLPPYVAPNPEA
                     THVFERQHHHYVRHYKGKHVQGEQPPNLLGLSCEWTFLGVKRVDVRFTSTVADRDLVL
                     L"
BASE COUNT          508 a          881 c          841 g          498 t
ORIGIN      
        1 tgggctccgt cactgtcccg ccgccgacca gaagctcgtc cgagcaatcg gcgtcgggct
       61 gacctcgcgc gtcccgtcac gcgccgacta tcgcttcagc tcgccacgcc ctcaaggccg
      121 ggctcggtgc accggatacc ggtcagcgcc gctgcgatgg cttctagccc gaccaccacg
      181 tcaccgagta ggtgttcgag gcgctgcttt ggaccgtctg accaacggcg tcgcagcccc
      241 tccgcctggg tccacgttgc ttcgaacgcc agtcgtcccg acgctgtgaa atcgtacttc
      301 tgcggagtcc tccagccaga tttagcctgg tcctccagct ccttcgctgt ggggacatgc
      361 gccgaacgat cggcgtgctc taccagccag aactccaccg gttcccgatt gatcgtgacc
      421 agcaaggcgc accgccctcg gccgacttcc gagcgattgc gaagttcgac ctggtgaccc
      481 cgagccgtga gcgccttgaa cagggcatcg aggagcagaa gccctctccg ctcggtctcc
      541 ttcccgacct tgaaaacagc gtggccatca ccgcgaaccc cggacatccc gtgctcgtcc
      601 ggcctcagcg gtctcatctg cccgcgaatc tgcttcacaa cgggatgcgg ccggctcagg
      661 ccgtcgccca cggaaacgtc cggtggcctc ggccgatcac gaaccgtgcg ctccgacttc
      721 cggtgcctcg tcagcgtgat cgtggttggt gtgcgcggcc caggtttcgg tagcttgtca
      781 ggtcgaggtt tgaagccagc ggcgagctgc tgccagtaac cgcggctggg ccggggtatg
      841 ccgagcttca cgcagtactt ggcgaaggcc acgctcgaca ctccgaattc ctcggcgacg
      901 gccgtcatgg gcctcgacca taccaaggag tacagctcgt cgcgggtgag agtcacggtg
      961 tccatgacgg gagcgtcgca ccggccgagc atcaagtgaa tcctgacgat agctgggaca
     1021 tcgtctcgca catgaagcca atgtcttccg atgaaactgc gcgtcgccga ccttcgcgag
     1081 atcgttcgcg ccgtggtcgc agacgcgctc gctacccacg ctcagcacga cctcctcgac
     1141 ctgaagcagg tgttcgagcg ctaccgcgtc ggcagagatg ctctcgttgc ggcagccaag
     1201 cgcggggagg tcgagctttc ccagggccct cgacgcaaat tcttgattcg agcacgcgaa
     1261 ctcgaacagt ggctgacgag acggaagtac gtcccgcctg ctctggcgac tgccaaggac
     1321 ttgcaggaat gggaccggca agcagacctg gccctggaac gagcgctggt gcaaggacga
     1381 cttcggcagc tttcgccccc cgaacttgaa gaggcacggg cgagacgctc gcgaaagaag
     1441 cgcccctagg ttgtggtcgg agacgcgagc ttttggcagc ggaactcggc tacagcagca
     1501 ccagatcccg gtcagccacg gtgctcgtga aacgcacgtc tactcgcttc accccaagga
     1561 acgtccattc gcacgacaga ccgagaaggt tgggaggctg ttcaccctgg acgtgcttcc
     1621 ccttgtagtg ccgcacgtag tgatgatgtt ggcgttcaaa gacgtgggtg gcctcgggat
     1681 ttggtgcaac gtagggaggc agcgtgggcg tctcgactgg gacagcccct agcacgttac
     1741 ccagaagcat ctgcacagtg aggctgccac tcggcgcata gtgagcgagg gcgaactggg
     1801 cggcggggtt ctggaccacc tcgaactcgt gtccgagcga gatgtctttg ccgttcgagc
     1861 atacttgcgt tcgaaagagc agagccgccg cgacgctcgg atggcggcca agacggctac
     1921 tcaatgcgta cgccctcgct tgttgcatcg acacttcctc aggcacgctc atggcaatta
     1981 cggagccctc gtcagccgtc gccgatgaaa agtgccgagc gagatttgtg cacgcgtcgt
     2041 cgatcttgtc gtcgaaacgc ttctgctcat ccacgtggag cggcacgaat acgctcagcc
     2101 cgactgaaac cttctcgtcg ctgaactcgg cattgttaag acggcccaag ggaactaccg
     2161 ctattctcca agacccgtcg cgcatccaga ggcacgcgct tgtggtgaca acgctgcacc
     2221 ctcgggcgag gctcagaagt tgctccgcag gtggtagggg tgcctttgcc gtgattgctt
     2281 ccaggaccaa gtgctgagcg cagccggatg gcagttgttg cctgagtacc tcgaacgccc
     2341 gtcgcgcgtc ggccttcagc gcggcgtcgt aggcgctcga accaagagcc ttgcagctta
     2401 cccgcaaacg cttgccggag gggagcgtga gcacgatgtc gtagcctggc tcgttcggcg
     2461 caggaacgtg aaccgtctgt cgttcggcgg cgagcatcga ggcaagcaag atctcaaatg
     2521 ccgagcctcg cgcaccgcga acatcgtgct gtctcatcct tcgggcgtgg tcgtcgaccc
     2581 acgccgtcgg aaacaaacct cgtgcatgct gaagggcgcg accgaaggtc aacagctcag
     2641 aggaggccaa ccaatcggtt cggcgccaga gctgaacgag aggattcccc tcgttcgatg
     2701 ggtccttcaa ccaggcttcg ccgaaaaa
//
LOCUS       DASTKU010000203        12867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1378263, whole genome shotgun sequence.
ACCESSION   DASTKU010000203 DASTKU010000000
VERSION     DASTKU010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378263"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VFQ61_07715"
     CDS             complement(<1..976)
                     /locus_tag="VFQ61_07715"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET9954374.1"
                     /translation="MTDREALQRRLLATFKVEAREHLEALSRGLMDLEQAEASAATEL
                     VETVFREVHSLKGAARSVGALDIESVCQALENIFSALKRGKLQRSASLLDRLLEAQRF
                     LEQLLTALESGRLNSSAGVDALVAALAALLRVEGSGQLQPGKPDQLRTPNPRGSGPVE
                     GAHQPLELRAAAPGAAEGKVSGSPASAFAELAAAVRDLTLGSAKAEANREPAPPEISA
                     GMSRPDALPPGAAESLASSADLHQVDQARSTPEAPERPPAPWAAHANLSRSDARAPSP
                     AAPVETVRVATERLDQVRLLAEDLLMEKLSLSQHAADLTRLAERLRESR"
     gene            complement(985..2715)
                     /locus_tag="VFQ61_07720"
     CDS             complement(985..2715)
                     /locus_tag="VFQ61_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HET9954375.1"
                     /translation="MIKWSIGHKIAGGFALAVAALLLVGFLSHRSTTRLVESAEWVEH
                     THRVLSLLEAVLANASESETAGRGFVITGDPVYLQGHEGARERALRAINEIATLTTDN
                     AVQQRRITSLEPLVTARFEALERVVATRRAAGFDAAQKLILEDRGRTLMSEVRRVISQ
                     MQTEENRLLLARVADERARAAATETTIVWSIGSSAVFLSWIGFLIAIGISRPLGALSR
                     AAQSISAGDLNVELPSAARSDEVGVLVRSFQVMSEWLERMARVATRIAAGDLTEGLAP
                     ASDKDRLGTAFVSMQQSLRKSTNDLREAANVLAAAAAEILASSTQMASGASEAAAAVT
                     ETTTTVEEVKQTAQLSSDKARLVSDNAQKANQIGQDGRRAVEGSIEGLERISQQMGAI
                     AEMVVKLSEQSQAISDIVATVTDLADQSNLLAVNASIEAARAGEQGRAFGVVAQEVRS
                     LAERSKQATAQVRGILTDTQRAISGAVLATEQGSKIVRGGVAQAHEANGAIRTLVETI
                     ELGAQAAMQIAASSQQQSIGMDQIAIAMDNIRQASLQHVSGTKQSEVAARNLHEVGQK
                     LKELVGRYRL"
     gene            complement(2824..3393)
                     /locus_tag="VFQ61_07725"
     CDS             complement(2824..3393)
                     /locus_tag="VFQ61_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9954376.1"
                     /translation="MYDPFSYPEREIALLKQRAREIAEAPPLETEPESSLSVLEFRLA
                     HEAYALDMASIAQVLALRDYAPLPFAPKMLLGIAYLRGQMLSIFDARPLLDLPAPGLT
                     ELNRVVVLASNGREIGMVVDSIERVVRVRKDQISDRLPAHAERHDYCVGLIPSGATLL
                     EATRLMAALFGDSALGDRALGDRAALTRA"
     gene            complement(3485..4951)
                     /locus_tag="VFQ61_07730"
     CDS             complement(3485..4951)
                     /locus_tag="VFQ61_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET9954377.1"
                     /translation="MNVGLTTPEVERARRWVAQRLGLEYSANRGLDLARGVEAAMSEL
                     GLQSFAAFEALLEASSPDSVPSSVTDVLAKHLTIGETYFFRDQAVFAALDQAILPALI
                     GRESPRRELRIWSAGCCTGEEPYSIAIAVDRALRDPERWEISILGTDVNPVFLRRAEL
                     AVFNAWSFRNVAPELRARYFTEREGQHFELDVKIRQMVRFARLNLVHDAYPSAMTGTQ
                     ELDLIFCRNVLMYLGAEVVQKVVERFRRCLRDGGFLVVSPTETSVELFRQFESKFLRG
                     AIVYQRRDARLDPKLELRLPDRSAGTRPSAATRAMDDAKVSPGSRSGPRPSCDRASER
                     VPVPARAALPSARPSEADPPISGEPPSLFTQARQAANSGNLKQALELCERALGGSKLD
                     CSLHYLRGSILAELGRSNEAVQALERALYLDPDFVLAHFSLGHLAFQRGQLRTARRYL
                     GNVLTLTREMDPDQELAEAGGLRAARLAQIAESLLGML"
     gene            complement(4948..5535)
                     /locus_tag="VFQ61_07735"
     CDS             complement(4948..5535)
                     /locus_tag="VFQ61_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9954378.1"
                     /translation="MTGDSQIVICSLDGQRYGLWLSAVERVIRMPWITPLPDAPPIVL
                     GVVKIRGELRAVVDLRQRFQLPARAVRASDRLLLARTAQRSVALRVDDVLGVETLRRE
                     DWVEAERLLPVTGMAPSASPDLGGLSLLGMGEPRGMVGVVQRGDLTFIHDLDHCLSSA
                     ERTALQHALQSHASESYTAGDRDAGNRDAEDPEVP"
     gene            complement(5532..8036)
                     /locus_tag="VFQ61_07740"
     CDS             complement(5532..8036)
                     /locus_tag="VFQ61_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9954379.1"
                     /translation="MTRVLIVEDSATQAEELAYLLESRQFEVVIRRDGLSGLEYCRTE
                     AVDVVLSDVIMPGIDGYELCRRLKTEEGTRDLPVILLTSLTDPLDIIRGLECQADNFV
                     TKPYDVDYLVARVNQLLENRRLRSQRKVNVGVDLVLMGRRFTINSEKEQMIDLLLSTF
                     EEVLRSRQREYEAKVAQETHRRSHKLLQSALDALSSQIATVDDAGEIVATNAAWRRMG
                     ELSGWRWPAAGVGQDYRVVLEQGFGAAREHALAALEGVEAVRRGEREFFHLEYPAQIA
                     GVARWHSIAATCFEGNGVRMLALENEDITGRKQATEEVRRSELALRRQTHILNSILNS
                     MSEGIIVADREGGLLLTNRRAEESLGVRGGMTREQWIPILEPYQPDSSDAESEAKSGA
                     TSAKSLNAAMLGELQGEFDVSSSGPDAKVLAVSGAPLKDEAGTLDGSVMVVRDVTQQR
                     KLEQQLRQAQKMEAIGQLAGGVAHDFNNLLSVITSYGQLMLRDLAEGDSKREDMEQML
                     EAAKRASGLTRQLLSFSRRQLVHATVLDLNQVVENVEKMLRRIIGEDIDLETVLSPEL
                     SRIKADAGQIEQVLLNLSVNARDAMPRGGKLTIQTSNVVLSSSFSAARMLAKSGPFVL
                     LTVSDTGVGMDSATQARIFEPFFTTKEVGKGTGLGLSTVYGIVNQTGGHIWVYSELGC
                     GTTFKVYLPAVDEELAAQTVAPPKSPTPANEQILLVEDEEAVRQVAARILREEGYRVL
                     VARGAAEARALCKEVGGRVDLLLSDVVMRDTTGPDLAAELSAVYPHLRVLYMSGYSEA
                     AVFRHGSLENGAAFLEKPFAPESLTRKVREVLETPR"
     gene            complement(8033..9076)
                     /gene="cheB"
                     /locus_tag="VFQ61_07745"
     CDS             complement(8033..9076)
                     /gene="cheB"
                     /locus_tag="VFQ61_07745"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HET9954380.1"
                     /translation="MSIRVLVAEDSATARALLVSILESDSELEVIGQAKNGHEAVALA
                     KRLLPDVITMDIHMPGLGGFEAIRRIRHDAPAPIVVVSASISKVDVAASLAALNAGAL
                     TALAKPAGPQAPNFEECARQLILTVKGMSEVKVVRRWSSVPARSAPRAIARPSKLLAI
                     AASTGGPAALYRIFSALPAKFPVPIVVVQHISHGFLPGMIEWWRSASALDMRVASSGE
                     VLQPGTVYVAPDSAHCQLSNRNTIVLSSAPPVDGHRPSASVLFRSAAQVHGTGLFALI
                     LTGMGRDGVDGLREVHARGGYVVAQDRESSVVFGMPGAAIEAGVVDDIVPLPAIADHL
                     MQLLITPTSREIR"
     gene            complement(9515..10999)
                     /locus_tag="VFQ61_07750"
     CDS             complement(9515..10999)
                     /locus_tag="VFQ61_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9954381.1"
                     /translation="MTDSAHKLSFKEKAGYAVGDAAANFVFQLLLVFQAGFYTDVMGI
                     SAAALGTLLLLVRVSDAFVDPLIGILADRTKHRWGKFRPWVIWSALPFAFLFWAAFTV
                     PPGTGTTGRLVYAVLTYTLLMIVYSVNNVPYSALMGVMTGDPSERTSLSSYRFVAGIG
                     AAFIVQGFTLPLVGKFGGGDDAKGWSLTIAIFAIVSIAFFLITFFTAKERIQPPADQQ
                     HNIKRDIADLKQNTAWLAMFFMTLFVFITLALRGGSYFFYFTYYVDSAALSRFVENIG
                     LVASPEEMQSFGAKFLDLFGLIVKPGGDPSKVGFSLFNMTGQLINILGILVAKPLADR
                     FGKKAVFTTGLAGATLVQFAFIILSPTAVGAMFLLNALSSLAYGPTIPLLWAMIGDTA
                     DYAEWKTGRRTTGFVFAGVVFALKAGLGLGGAISGWLLAFYGYTPETAKSPEVLSGIR
                     SMASIYSAIPFAIGVVCMLYYPITKALNLQISRELEERRRVATA"
     gene            12093..>12867
                     /locus_tag="VFQ61_07755"
     CDS             12093..>12867
                     /locus_tag="VFQ61_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HET9954382.1"
                     /translation="MSRSPLVSHIYTADPSAHVFEGRVYIYPSHDLDIDAEDNDLGDQ
                     YAMTDYHVLSHDHAGAPTTDHGEALHIRDVPWALKQMWAPDAAYRNGKYYLYFPARDK
                     DGIFRIGVATSTRPEGPFKAEPEPIRGSFSIDPCVFGADDGEYYMCFGGLWGGQLQCW
                     ATGHFVANAQTPPADQRALCPQIARLTPDMREFASPPRDLVILDSQGEPLKAGDTERR
                     YFEGPWMHKYRGLYYFSYSTGDTHTLVYATSESPTGPFTF"
BASE COUNT         2403 a         4264 c         3858 g         2342 t
ORIGIN      
        1 ggcgtgattc gcgcaggcgc tccgccaagc gggtaaggtc tgcggcatgc tgactcaagc
       61 tcagcttctc catcaggagg tcctccgcta gaagccgcac ctgatcgagc cgttccgtcg
      121 ctacacgcac cgtttccacc ggcgcggcgg gactgggcgc acgggcatcg cttcgggata
      181 ggttcgcgtg agccgcccag ggcgcggggg gacgctctgg tgcctccggc gtcgacctgg
      241 cttggtctac ttgatggagg tctgccgacg aagcaagcga ttccgctgct cccggcggca
      301 acgcatcggg tcgcgacata cccgccgaga tctctggtgg cgcgggctcc cgattggcct
      361 cggccttagc ggaacccaga gtaaggtcgc gcaccgccgc cgcgagctcg gcgaaggccg
      421 acgcgggtga cccggacacc ttgccttcgg ctgcccccgg tgctgcggcg cgcagctcga
      481 ggggctggtg ggcgccttcg accggaccgc tcccacgcgg gttgggagtg cggagctgat
      541 ctggttttcc cggctgtagt tggcctgagc cctccacgcg aagcagcgcc gcgagcgccg
      601 cgaccagcgc atccacgccc gccgaggaat ttagcctccc gctttccaac gccgtcagta
      661 gctgctcgag gaaacgctgc gcctccagca agcgatcgag caagctggcg ctccgctgca
      721 gcttgccacg tttgagcgca gagaagatgt tctcgagcgc ttggcacacg ctctcgatgt
      781 ccagggctcc caccgagcgc gctgccccct tgaggctatg gacttcgcgg aacaccgtct
      841 ccacgagctc ggtggccgcg ctggcctccg cttgctcgag atccatgagc ccgcgactga
      901 gtgcctcgag gtgctcgcgg gcctcgacct tgaacgtcgc gagtaggcgt ctctggagtg
      961 cctccctgtc ggtcatggtg cggttcagag ccgatagcgg ccgactagct ccttcagctt
     1021 ctgcccgacc tcgtgcaggt tacgcgcggc gacctcggac tgtttcgtgc cgctcacgtg
     1081 ctgcaggctt gcctgacgga tgttgtccat cgcgatcgcg atttgatcca ttccgatcga
     1141 ttgttgttgg ctcgatgccg cgatttgcat ggcggcttgg gcgccgagct cgatcgtctc
     1201 gactagggtg cgaatcgcgc cattggcttc gtgcgcctgg gcgaccccgc cgcgaacgat
     1261 tttgctgcct tgctccgtcg ccaggacggc gccgctgatg gcccgttggg tgtccgtcaa
     1321 aatgcctcgc acttgtgcgg tggcctgctt ggaccgctcc gcgagtgacc tgacctcctg
     1381 agccaccacg ccgaaggctc ggccttgctc gccggctcgc gctgcttcga tgctcgcgtt
     1441 gacggcgagc aggttcgatt ggtcggccaa gtcggtaacg gtggcgacga tgtccgagat
     1501 ggcttggctc tgttcgctca acttcacgac catctccgcg atcgcgccca tctgctgcga
     1561 aatgcgctcc aacccctcaa tcgagccttc caccgcgcga cgcccatctt gcccgatctg
     1621 attggctttc tgtgcgttat cagacaccag ccgtgccttg tcgctcgaaa gctgggcggt
     1681 ctgcttgacc tcctcgaccg tcgtcgtggt ctcggtcacc gccgccgctg cctcgctcgc
     1741 ccccgacgcc atctgggtac tgctcgccaa gatttcggcg gcggcggccg cgagcacgtt
     1801 ggccgcctcg cgcagatcgt tcgttgattt ccgcagactc tgttgcatcg agacgaaggc
     1861 ggtacccagt cgatctttgt cggacgcagg ggccaagcct tccgtgagat caccggccgc
     1921 gatccgcgtg gccacgcgcg ccattcgctc gagccactcc gacatgacct gaaacgagcg
     1981 gaccaagacc cccacctcgt ccgagcgcgc cgcgctcggc aactccacgt tcaagtcacc
     2041 agccgagata ctttgcgcgg cgcgactcaa ggcaccgagt gggcgcgaga tgccgattgc
     2101 aatcaagaaa ccaatccagg acagaaatac tgcgctactc ccgatactcc agacgatcgt
     2161 ggtctcggtc gccgcggcgc gcgcccgttc atccgcgacg cgcgctagca acagccgatt
     2221 ctcctcggtt tgcatctgcg agatcacccg gcgcacctcg gacatcagcg ttcgaccgcg
     2281 atcttctagg atcagctttt gagccgcgtc gaacccagcg gcacgccgcg tcgccacgac
     2341 gcgctcgagc gcctcgaagc gcgcggtcac gagcggctcg agcgacgtga ttcgacgttg
     2401 ctgaaccgcg ttgtccgtag tcaacgtcgc gatctcgttg atcgcgcgca gggcccgctc
     2461 gcgggcaccc tcgtgccctt gcaagtacac ggggtccccg gtgatcacga agccacgacc
     2521 tgcggtttcg gactcggagg cgttggccag caccgcctcg agcaagctca atacgcggtg
     2581 cgtgtgctcg acccactccg ccgactcgac gaggcgcgtc gtgctgcgat gagacaagaa
     2641 gccgacgagc agcaacgccg cgaccgccag cgcaaaacca cctgcgattt tgtgcccaat
     2701 cgaccatttg atcatcgttt caccattccc gggtttcgcg ctggcgcaga acgcctattt
     2761 cgcggcttag ttcaacacga atgcgctccc gagcaccaat tcgtcgctcg atcgcgtcgg
     2821 cgctcacgcg cgtgtcagag ccgcgcggtc accaagagcg cggtcaccaa gagcactgtc
     2881 gccaaatagc gcggccatga gccgagttgc ctccagcaac gtcgcccctg aaggaatcag
     2941 gccgacgcaa taatcgtggc gctccgcgtg cgcaggcaga cggtcggaaa tttggtcttt
     3001 tcggactcga acgactcgct cgatggagtc tacgaccata ccaatctcgc ggccgttcga
     3061 ggccagcacc accacgcgat tgagctccgt aagccctggc gccggcagat ccaagagcgg
     3121 gcgagcgtcg aagatcgaca gcatctgacc acggaggtac gcaatcccga gcaacatttt
     3181 gggggcaaaa ggtagcggag cgtaatctcg cagcgccaag acctgcgcga tagaggccat
     3241 gtccagggcg tacgcctcgt gcgccaagcg aaactcgagc accgagaggg agctctccgg
     3301 ttcggtctcc aacgggggcg cctcggcgat ttcccgagct cgctgcttga gcagcgcgat
     3361 ttctcgttct ggataggaga aggggtcgta cacggcttgc tacctcgttc ccggatttct
     3421 ccggggctcc tctcgaatca tgcgtttcac gaccgagccg atgcgcgtca cccatcggct
     3481 tggatcaaag catccccagt agactctccg cgatttgtgc cagccttgcg gcgcgcaggc
     3541 cgcctgcttc tgcgagctct tggtctggat ccatctcgcg agtcagggtc agcacgttgc
     3601 ccaagtatcg gcgcgcggtg cgaagttggc cccgttgaaa ggccaaatgt cccagcgaga
     3661 agtgagctag cacgaagtcg ggatccaagt acaaagcccg ctcgagagcc tgcaccgctt
     3721 cgttagatcg tcccagttcg gctaaaatgc tgcctcgaag gtaatgtaag gagcagtcca
     3781 acttggagcc gcccagcgcg cgctcgcata gctccaacgc ctgcttcaaa ttgccggagt
     3841 tagcggcttg ccgcgcctgg gtgaacaaac tcggtggctc gcccgagatg ggcgggtccg
     3901 cttcgctcgg ccgcgcacta gggagagctg ctcgcgcagg tacgggaacc ctctccgaag
     3961 ctcgatcgca agagggcctc gggcccgatc gcgatccggg gctcaccttg gcgtcgtcca
     4021 tggcgcgtgt cgccgcgctg gggcgagtgc ccgcggaccg atcaggaagt cgcagttcga
     4081 gcttcggatc caggcgcgcg tcccggcgtt ggtaaacgat cgcaccacgc agaaacttgc
     4141 tctcgaactg ccggaacagc tcgaccgaag tttcggtcgg gctcaccacc agaaatcccc
     4201 catcgcgcag acagcgccgg aagcgctcga ccactttttg aacgacctct gccccgagat
     4261 acatcaacac gtttctacaa aatatcaggt ccagctcttg cgtacccgtc atggccgatg
     4321 gataggcgtc gtgaaccagg ttcaggcgcg cgaagcgcac catctgacga atcttcacgt
     4381 cgagctcgaa gtgctgcccc tctcgctccg tgaagtaccg cgcccgaagc tcgggtgcca
     4441 cgttccggaa tgaccaggca ttgaagaccg caagctcggc acggcgcaag aacacggggt
     4501 tgacgtcggt tcccaagatc gagatctccc agcgctcggg gtcccgcagc gcacgatcga
     4561 cggcgatcgc gatggaatag ggttcttcgc cagtacaaca accggcgctc cagatcctca
     4621 gctcgcggcg gggactctcg cgcccgatga gcgccggcag aatggcctga tcgagcgcgg
     4681 cgaatacggc ctgatctcgg aagaagtacg tttcaccgat cgtcaggtgc ttcgcaagca
     4741 cgtcggtgac cgagctcgga accgagtccg gagaactggc ttcgagcagg gcctcgaatg
     4801 ccgcgaagct ctgcaagccg agctcgctca tggcggcttc gacacctcgc gccaagtcga
     4861 gcccacggtt cgcggaatac tccaagccca agcgttgagc gacccagcgc cgagcgcgct
     4921 ccacctccgg cgtggtgaga cctacgttca tggcacctct ggatcttccg catcgcggtt
     4981 ccccgcgtca cgatctcctg cggtgtagct ctccgacgca tggctctgga gcgcgtgctg
     5041 cagtgcagtc cgttcggcgg acgagaggca atggtccaag tcgtggatga acgtcaagtc
     5101 cccccgctgc acgacgccca ccatgccgcg gggctccccc atccccaaga gtgataaccc
     5161 accgagatcg ggagaagcgc tgggagccat cccggtcacg ggcaacaaac gctccgcttc
     5221 cacccaatcc tctcgtcgca gagtttccac gcccaaaacg tcgtcgaccc gcagggctac
     5281 cgagcgctgc gccgtgcgag ccagcaacag gcggtcgctt gctcgaaccg cgcgggcggg
     5341 cagctgaaaa cgctgacgga gatcgaccac ggcccgcagt tcaccgcgga tcttcaccac
     5401 tccgagcacg atcggtgggg catctggaag gggcgtgatc caaggcatgc gaatgacgcg
     5461 ctccaccgcc gacaaccaca agccgtaacg ctgcccgtcc agcgaacaga tcacgatttg
     5521 tgagtccccg gtcaccgtgg cgtctccaac acttcgcgca ctttgcgcgt caaggactcg
     5581 ggggcgaacg gcttctccaa gaaggccgct ccgttctcga gtgatccgtg ccggaacacc
     5641 gcggcttcgg agtagcccga catgtacagc accctcaagt gcggatagac cgcggacagc
     5701 tcggccgcga gatccggccc tgtcgtgtcg cgcatgacca cgtcgctcaa cagcagatcg
     5761 actcttccgc ccacttcctt acacagcgcg cgagcctcgg ccgcgccgcg cgcgaccagc
     5821 acgcgatagc cctcctcacg cagaatcctg gctgcgacct ggcgcacggc ttcttcgtct
     5881 tctacgagca agatctgttc attggcaggc gttgggctct tcgggggcgc aacggtctgc
     5941 gccgccagtt cctcgtccac cgccggcaag tagactttga acgtcgtccc gcagccgagc
     6001 tcgctataca cccagatgtg tcccccggtt tggttcacga tgccgtagac cgtggagagc
     6061 ccgagcccgg tgcctttgcc gacctccttg gtcgtgaaga agggctcgaa gatcctcgcc
     6121 tgagtcgcgc tgtccatgcc cacccccgtg tcgctcaccg tgagcagcac gaatggccct
     6181 gacttcgcaa gcatgcgcgc ggcgctgaac gagctgctca acacgacatt gctagtctga
     6241 atcgtcagct tcccgccgcg cggcatcgcg tcacgcgcgt tcacgctcag gttcaagaga
     6301 acctgctcga tttgccccgc gtccgctttg atgcggctca gctcggggct gagcacggtc
     6361 tcgagatcga tgtcttcgcc gatgattcgg cgcagcatct tctcgacgtt ctcgaccacc
     6421 tgattgagat cgagcaccgt cgcgtgcacc agctggcgcc gactgaagga cagcagctgc
     6481 cgagtcaggc cggaagcccg cttggccgcc tcgagcattt gctccatatc ctcgcgcttg
     6541 gaatcccctt cggccaagtc acgcaacatc agctgaccgt aactggtgat caccgagagc
     6601 aagttgttga agtcgtgagc gacgccgccc gccaactgcc cgatggcttc catcttctgt
     6661 gcctggcgca gctgctgttc gagcttgcgt tgttgagtga cgtctcgaac caccattacg
     6721 ctgccgtcca gcgtgcccgc ctcgtccttg agtggggcgc cgctgacggc cagcaccttg
     6781 gcgtccgggc cggaactgga gacgtcgaat tcaccttgca gctcgccgag catggccgcg
     6841 ttcagcgact ttgctgatgt cgcgccgctc ttggcttcgc tctcggcatc cgaagagtcc
     6901 ggctggtacg gctccaggat cggaatccat tgctcccggg tcatgccgcc cctcacgccg
     6961 agtgactcct cggcgcgccg gttggtgagc agcagccctc cttcacgatc cgcaacgatg
     7021 atgccttcgc tcatgctgtt caggatcgag ttcaagatgt gcgtctgtct tcgcaacgcc
     7081 agctcggaac ggcgcacctc ctccgtcgcc tgcttgcgcc ccgtgatgtc ttcgttttcc
     7141 agagccaaca tgcgcacgcc gttgccttcg aagcacgtgg ccgcgatcga atgccagcgc
     7201 gccacgcccg cgatttgcgc ggggtactcg aggtggaaga actctcgttc gccacggcgg
     7261 accgcctcta ccccctccaa agccgccagg gcatgctcac gcgcagcccc aaagccctgc
     7321 tccagcacca cccggtagtc ttgaccgact ccggccgcgg gccagcgcca gcccgaaagc
     7381 tcgcccatgc gccgccacgc ggcgttggtg gctacgatct ccccggcatc atcgacggtc
     7441 gcaatttgcg aagagagcgc atccagggcc gactgcagca gcttgtgcga gcggcggtgg
     7501 gtttcctggg ccaccttggc ctcgtactcg cgttgccgcg agcgcagcac ttcctcgaac
     7561 gtcgagagca gcagatcgat catctgctcc ttctcggagt tgatcgtgaa gcgccgcccc
     7621 atcaacacga ggtcgacccc gacgttcacc ttgcgctgcg aacgcagcct gcgattttcg
     7681 agcagttgat tgacccgcgc gaccaggtaa tccacgtcgt aaggcttggt gacgaagttg
     7741 tccgcctgac actccaagcc ccgaatgatg tcgagcggat ccgtcaaaga cgtgagcagg
     7801 atgacgggga ggtcgcgcgt gccctcttcg gttttcagcc ggcgacacag ctcatagccg
     7861 tcgattcccg gcatgatcac gtcgctcaac acgacatcca cggcttcggt tctgcagtac
     7921 tccaggccgc tcaagccgtc acggcggatc acgacctcga attgacgtga ctccagcaga
     7981 taggcgagct cctccgcctg cgtggcgctg tcttcgacaa tcagaacccg agtcaccgga
     8041 tctctcgcga ggtaggagtt atgagtaact gcatcaagtg atctgcaatc gcgggcagtg
     8101 gcacgatgtc gtcgaccacg cccgcctcga tcgccgctcc gggcattccg aagacgaccg
     8161 aggattctcg atcttgagcg accacgtatc caccacgtgc atgcacttcg cgcaagccat
     8221 caacgccgtc acgacccatg cccgtcaaga tcagcgcaaa taacccggtg ccgtgcacct
     8281 gcgcggcgga tcggaacaaa acactggcgg aagggcgatg gccatcgacg ggcggtgcgc
     8341 tcgagagcac gatcgtattc cggttcgaca gttggcaatg agctgaatcg ggtgccacgt
     8401 acaccgtgcc gggctgtaac acctcacctg agctagccac gcgcatgtct aaggccgagg
     8461 ccgatctcca ccactcaatc atgcctggta gaaacccgtg tgagatgtgc tgtacgacca
     8521 caatcggtac cggaaacttg gcgggcaggg cggagaagat tctgtacaac gcggcggggc
     8581 cgccggtaga tgcggcgatt gccaacaact tgctcggacg agcgatggcg cgcggagcgg
     8641 agcgcgctgg cacactcgac cagcggcgca cgaccttgac ctccgacata cccttcaccg
     8701 tgaggatcaa ttggcgcgca cattcctcga aattcggcgc ttggggaccc gcaggcttgg
     8761 ccagggccgt gagggcaccc gcattcagag ccgccagcga cgccgccacg tcgaccttac
     8821 tgatgctcgc ggagaccacg acaatgggtg cgggagcgtc gtgcctaatc cgccgaatgg
     8881 cctcgaaccc tccgaggccc ggcatgtgga tgtccatcgt gatcacgtcg ggaagcaacc
     8941 gcttggcgag cgcaaccgcc tcgtggccat tcttggcctg accgatcacc tcgagttccg
     9001 agtcggactc gagaatcgac accagcagcg cgcgggccgt tgccgaatcc tctgcaacga
     9061 gcactcggat gctcatgaga cctgtgcgcc tggttgcaga ctccgcatca ggcgttcggt
     9121 cgagagccca gtatcgatat tcgataaccc tggcgccaag cgccgggcga ccaccgaaca
     9181 ccgtgacttc ccgcgtgcgc cggatcgaaa tctgggcaaa catccgcgag ccggagcgcg
     9241 cacgcatggc cttttcgcgg tcacctcgct gtgagcggta gggtccaaca cggatgccgc
     9301 cttcgagatt agggcaagcc aatccgctgg aacacagtca tttttgcccc gcgaggaagg
     9361 aggaatacat tgagcgcatg ggtggataca caccccgcgt aaacacaccc tacgagtaca
     9421 aacgaagaaa cccggcggct ccttcgggag caacgccggg tcttcttcgg ctggcggccg
     9481 gttcccactc tcgagcggga acgtgaaccg ccgctcaggc ggtcgcgacg cggcgccgct
     9541 cctccagctc gcggctaatc tggagattca gcgccttcgt gatggggtaa tacaacatgc
     9601 acaccacacc aatcgcgaat gggatggccg aatagatgct cgccatactc cgaatcccgg
     9661 acagaacttc cggtgacttg gccgtttcgg gtgtgtagcc gtagaacgcg aggagccaac
     9721 ccgagatcgc gccgccaagc ccaagcccgg ccttgagcgc gaacacgacg ccggcgaaca
     9781 cgaaaccagt cgtacgccga cccgtcttcc actctgcata atcggccgtg tcaccaatca
     9841 tcgcccaaag taggggaatg gtcgggccgt acgcgaggct cgagagggcg ttcagcaaga
     9901 acatcgcccc gaccgcggtc ggtgaaagga tgatgaaggc gaactggacg agcgtcgcgc
     9961 cggcgagccc cgtcgtgaac acggcctttt tgccgaaccg atccgcaagc ggcttggcga
    10021 cgaggatgcc cagaatattt atcagctgcc ccgtcatgtt gaacaagctg aagccgacct
    10081 tcgaggggtc cccaccgggc tttacgatca gaccgaatag gtccaaaaac ttggcgccga
    10141 agctctgcat ctcttctgga ctggcgacca agccgatgtt ttcgacgaac cgactcagcg
    10201 cggccgagtc gacgtagtag gtgaaataga agaaataaga gccgccgcgc agagccagcg
    10261 tgatgaacac gaacagcgtc atgaagaaca tcgccagcca ggccgtgttc tgcttcagat
    10321 cggcgatgtc ccgcttgatg ttgtgttgct gatcagccgg aggctggatg cgttccttgg
    10381 ccgtgaagaa cgtgatcaag aagaaggcga tcgacacgat ggcgaagatc gcgatggtca
    10441 gagaccagcc ttttgcgtca tccccaccac cgaacttacc gaccagcggg agcgtgaagc
    10501 cctgcacgat gaaggccgcg ccgatgccgg ctacgaatcg gtaagacgag aggctggttc
    10561 gctcactcgg atcgcccgtc atcacgccca ttagcgccga gtacggcacg ttgttgacgc
    10621 tatatacgat catcaacagc gtgtacgtca gcaccgcgta taccaggcgg ccggtggtcc
    10681 ctgttcccgg gggtacggtg aatgctgccc agaagaggaa cgcgaagggc aaagcagacc
    10741 agatgaccca cggcctgaac ttgccccaac ggtgcttggt gcggtccgcg agaatgccga
    10801 tcaacggatc tacgaaggcg tcgctgaccc gcaccagaag cagcagagtg cccagcgcgg
    10861 ccgcgctgat acccatgacg tcggtgtaaa aacccgcctg gaataccaac agaagttgga
    10921 aaacgaagtt ggcggcggcg tctccaacgg cgtagccggc cttctctttg aacgagagtt
    10981 tatgtgctga gtcagtcatg ggcgtgcaac gcaatcatgg gggtcgaaga gatctccgcc
    11041 gcagagaagg cgggcaagct gaggcatgag agcccgggac tctagaccga attgcacgcc
    11101 aaaacacaga aaaactctgc cgagggctca ggcgtcagct agcgatcgcg gagagcccac
    11161 ggcgtgcctg ccgcctgggc aacgcgaggc tcacccaacc gtattgggac tcgaagctcg
    11221 acggttggta gtccgcgcaa gtcatcctat ttggaagact catctcactc tcgccatcgc
    11281 ggcggcccaa gagcaatctg taccgagggt gaacccgcgg tgtgactcgc gcgtgcctgc
    11341 cctattccgg tttagccgca gttttgcggg ttgtgcggct cgcgacggcg tgcgccacgc
    11401 agtgcggcaa gtccggccga gcggttatct cgtaccgctc aggcgcggga agcatgcccc
    11461 tcttacatcg atcttccgcg ggtattgccc cctgtacctg acaccttcca ccgaaggcca
    11521 atcgactact tcgttctaca ctctgtctta gccctctgcg tcttagccct ctgcagcaac
    11581 actcccgcgc ccagaggcac agaactcgag ttgcccgagt ttctcgagga ccagaggact
    11641 cgatgaacct cgcgccaaag ggttcggcga tgacggagcg gtttgaagaa ctagtgtcaa
    11701 aaaagatgga gctgcgcccg gaagcggtct tgggcccgat ccctagagga gggccgccga
    11761 ctccctccca ttggaagggg ctcttggagc agctctcgac gagccgcacg ggcctttaag
    11821 agaagcctcg gcatcgaaca ggaccggacg ctcccctcct gcctagcttt cagccctatt
    11881 cgtgagccgg ccactttggt gctgccgatt ctgagctcgc gttggcaatt ctgcgcgggc
    11941 gctgccaatt ccgccgcccc tgactgcggt gcctgtccga tctctgcgtt ctatctctgt
    12001 tggctggctc ggaggcgttt tctgtagcat agccgtgggc aaccggactt tgcccaacta
    12061 ggtcccgagc cgcacccgcc gagtaacttc ccatgagtcg atctcctctg gtttcgcaca
    12121 tctatacggc cgacccctcc gcgcatgtct tcgaaggccg cgtgtatatc tatccgtcac
    12181 acgatctcga catcgatgct gaggacaatg acctcggcga tcagtacgcg atgacggact
    12241 atcacgtgct ctcgcacgat cacgcgggcg caccgacgac ggaccacggg gaagcgcttc
    12301 acatccgcga cgtaccttgg gccctcaagc agatgtgggc gcccgacgct gcgtaccgca
    12361 atgggaagta ctacttgtat tttccggccc gtgacaaaga cgggatcttc cggattggcg
    12421 tcgcgacgtc gaccagacca gaggggccgt tcaaggccga gccggagccc attcgcggca
    12481 gcttcagcat cgatccctgt gtattcggcg ccgacgacgg cgagtactac atgtgcttcg
    12541 gtggcttgtg gggcggtcag ctccagtgtt gggcgaccgg tcacttcgtg gcgaacgctc
    12601 agactccgcc cgccgatcag cgcgcacttt gcccgcaaat tgcgcggctc acgccggaca
    12661 tgcgggagtt cgcgagcccg ccccgcgatc tcgtgatcct cgattcgcaa ggtgagccgc
    12721 tcaaggcggg tgacaccgag cgccgctact tcgaggggcc ctggatgcac aagtaccgcg
    12781 gtctctatta cttctcctac tcgacgggtg atacgcacac gctcgtgtac gcgaccagcg
    12841 aatcaccaac tggcccgttc acgttcc
//
LOCUS       DASTKU010000204        11225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1824545, whole genome shotgun sequence.
ACCESSION   DASTKU010000204 DASTKU010000000
VERSION     DASTKU010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11225
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824545"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="VFQ61_07760"
     CDS             complement(<1..1490)
                     /locus_tag="VFQ61_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HET9954383.1"
                     /translation="MTHAPSEAERLSERFLGLAPGARREFWRRLRERGLDSSLLPIPA
                     GAAGAETTLSYAEERIWFLSRLEPDSPAYHMVAALRIRGELDTSRVSGALRALAVRHP
                     ALRTRYIERDGVAHRHISTEPEIEVITLHVPEPARRSEAALRALTQPAITKPFDLARD
                     PLLRVVLARSGERECILILVIHHLVADGASLDILMREFASYYGGAISRHLDAAGCDPV
                     RLPALPVTYADYAVFQRALFEAGELEELIASWREGFCVEPPALQLPADHPRSAESYGR
                     GGQVQRTLRAKTWDRLRALARREGVTAFMLLIGAFAALLSRLSGEQRIVIGVPSGSRS
                     RSEIEGVVGLFVNTLLVDLTLDDDDDFHRLFQRTKRAVLDAQSHRALPFEKIVETIAP
                     ERRLGQSPLFQAMFNYQSRTMHGLGLDLEMLRWGNGAAQLDLSLDTEDVDGTLFASFT
                     YDASRFAAPRVELWADAWVALLESACRESGCSSVRACLPGSQERQLR"
     gene            complement(1487..11188)
                     /locus_tag="VFQ61_07765"
     CDS             complement(1487..11188)
                     /locus_tag="VFQ61_07765"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9954384.1"
                     /translation="MFEESTIRAWVSAYLLLLARMVHAPERAIGEIGLVSDMQLASWC
                     EQAVREVDPEGEATPALLDEFLTTVGHQADCTAVIAGEERVSYAELEARARRIAAALA
                     RLGVGREQRVAIFASRSASLIAAMLGVWMVGGAYVPIDPEYPISRIQWMIADCGARVL
                     LADQSSSNLTDGGFTGTVIELASIDGSAELLRELTPSLPDQAAYLIYTSGSTGTPKGV
                     SVSQGALAMHCAAAARLYEMRRTDRALHMASMSFDAGVEQWVVPLVFGGGVVLSQGAW
                     SGAQIAEIVAREQISIVYPPTNYVVELARFGLSGGALGRVRVCTVGGEAVQTSSIELI
                     RAGLRPEKIINGYGPTEAVITPMLWQADAGPAGTPLAPIGRVVGRRSAWVLDSDLQPV
                     PEGTVGELYLGGEGLARGYHAQPGFTAARFVPDPFSAAPGARLYRTGDRARRRPDGVF
                     EYVGRVDHQIKLRGHRIELAEVEAELAALRGVKAAAAALRRLGSHPQLVGYVVLENTA
                     VLENTARLEQQLKDSMASRVPEYMVPGRILCLSALPTGPNGKLDRAALPEPAAVRREL
                     VPATNSVQEKLVELWQKLLQVEEVGVADHFFELGGDSVLAIQLVGRARQSGLVFTPRD
                     LFANPTIEQLARAIQQVGNEQVEALPEGRLPLSPIQSEFLCLRPSRPEHFNQAVLLEL
                     TREVSTAGLTTALEKLVAHHDALRLRFCFAEVGVAEPYQHYAHEEPTALVWRREVATD
                     AELQRECRLAHSSLDLENGPLLRAIHFLHSDGRRLVFLTIHHLVVDAVSFRILIEDLE
                     VLLNGGALSPKTASFGSWIRSLEQIASSAGFAAEREHWLSVLGDRTQAASHRVYRPSS
                     KLQFEVPASYREMVRRARAAGIPLQALLLGALAHSMQNAGDELWVELESHGRDAGATP
                     LDISRTVGWFTATYPLSLRPNGSLSDTVESARQELAAQSPHAWSYGVWRRFGSGTGQA
                     RSLNPKLTFNYLGRLDAGLRDSDWFRLVEPGSTVELRDPESPSLVPATLDLRVIGDRL
                     IGELSHDPEAWEPRAAEEFMERFRAAIAELVRRKSGTLRNSDFPAVRLSERELSSLPF
                     AADSVEDILPLTPIQQGILFHAVERGAADPYFFQRGFCFEGEWDGQRLQRAFRRVAER
                     HAALRTAYFWEFENGPVQVIMRAADVRWEEEDLSELSEADASRALGAHLRQTREAPFD
                     FARAHSLRFKWLQRGPSAGWLIWSHHHIALDGWSLAQVMSELLELYSNLEAPDPRAAS
                     YAEFVCRVTQADAGESLEYFREHLTGIEGPTALPRGKGDPNLEGQYGEYSLRVSCSQE
                     QRLRELAQGAGVTLATLFQAAWAIVLARHTGSQDVLFGVTVSGRPTDVPGIEHSVGLF
                     INTLPCRVVMPSESGVGEWLYALQELGATLREHGSVGLSEIQRTCGFGEGLPLFESVL
                     VIENYPISSVLRAHSAAHRVKLLEESAPIEHLLSRNGRNNYPLTVAVSFQDGIQIALS
                     HDLGRIDHVTAEALSRQLLGVAEALSAGREAKVGSIGLPIFGPSALLSEPASREPLDD
                     IVLNWRRRVAEAPEVISICSADDSLTRAELDAWSDEIAIALRAQEIGLESVVALCTPR
                     SAAFVAGVLGVLKAGAAYVPVEPETPTERRNFILRDSGACCILFSSAHRTLVEDELPG
                     LDIDALRDSSVRVRAGEGALPREFHRDAPAYLIYTSGSTGRPKAVSVSRGSLAHYLSG
                     LLERVSLPVQGAFAMASTVAADLGHTVLFGALVAGRALCLIPNESGFDPDAFASIMRT
                     HEVAVLKIVPSHLSGLLHAACPSDVLPSHTLVLGGEACSPSLLRKIRELRPDLAIINH
                     YGPTETTVGVLTHAVEVSDVERDPPLGRPLAGTTVQVLDAGLNLLPEGVAGELYVGGP
                     ALARGYQGQPGLTAARFVPDPAGSGRRIYRTGDRACVRAGRVEFLGRADSQVKIRGQR
                     VELGEIQAALRSLSEVRDAVVVTAPGEEDSLRLVGYYVAARPGGDGSQRALTIRETTE
                     VVRRQLERILPASLVPQLIEVAELPVTANGKIDLRRLPAVGLNQGGPSRSDLAQANSP
                     VEQLLLEIWAAVLKRQDIGIHDNFFELGGDSILSLQVIARARKQKLRINPKLLFQHPS
                     IAALARALGGATAEYSESEVGIPRASTSDRKLLSSVQQRLWMLSALEPESHAYVVGGA
                     VRLRGVLNRAALRSALTELAARHELLRSCFDSHETESGEVERRVLEQVETELTFTQCT
                     AATSSLRALEHEARRLATRELERPFDLSRAPLWRALCIQLSEEDYVIVLSAHHIVVDG
                     WSMNVLVQEFGERYAASVEGRSACLPNQTPNYTDFAAWQRQRLASSSVARELEYWRGA
                     FARIPEPLALPLDHVRPARPSSRGGSLLVDIPLELQGRLRRLAELHSATLFMVLLGGW
                     AALLQRWSGQQELCIGMPVANRRHPDTQELVGPLLNTLPLRLTLGGEESFASVLARTR
                     RAVLEAQEHQEVPFERIVEALRVERNSTRSPLFQVLMNHQRRDLAALRELAGLGLERF
                     ELPKTSAQFELALHTEEDDSGALRAEVVYSRDLFDETTIERLGAEYVRLLSDAVREPA
                     RPLAQFGRLSRSDQARLAAWNATQTDFGVPGSILDLLRHAAHTYADRVAVIFENDQIT
                     YRELDRRSDLLAMSLCRSGVGPDAVVAIGLERCLELVIALVAVLKAGGAFLPLDPSYP
                     DGRLIQMVAEARPRVLLTTRPLQTALAACANIPTLCLDEFKWVVEDGGDSRPIARVRP
                     ENLAYVIYTSGSTGLPKGCANTHGGLKNRLLWMQQQYGLAAHDVVLQKTPISFDVSVW
                     EFFWPLISGARLVLASPGEHRDPARLAALVVKHGITTLHFVPSMLQAFLNAPEARTCE
                     SVRRIVASGEALSVELRAACKAALPSANLFNLYGPTESAIDVTHWSCSSDDLPTVPIG
                     RPIANVRVHVLDAHLDPVPPGHPGELYLGGIGLARGYFARPSLTAARFVPDAVGPEPS
                     ARLYRTGDLARFRDDGALEYLGRVDHQVKLRGFRIELGEIEACLLEHPSVLEACAVLR
                     GTGEQAFLAGYYVPKRLGDVDALDHELCELLRLKLPAHAVPSFLVPLDAMPLSANGKL
                     DRKALPEPDRPRAASRLPGNETERAVAEVWEIVLGVQRLRAEDDFFALGGHSLLAVKA
                     AARLRERFQVALPLRKLFEATTVATLAAAIDLERGATVTNPLAALDELMSELEVEPS"
BASE COUNT         2243 a         3714 c         3339 g         1929 t
ORIGIN      
        1 cggagctgcc gttcctgact acccggcaaa cacgctcgta cactcgagca gcccgactcg
       61 cgacacgcac tttcgagcaa cgcgacccaa gcgtcagccc agagctcaac cctcggcgcc
      121 gcaaaccgac tcgcgtcgta ggtgaaggaa gcgaacagag ttccgtccac gtcctcggtg
      181 tcgagactga gatcgagttg ggcggctccg tttccccaac gcagcatctc cagatcgagc
      241 cccagaccat gcatagtccg agattggtag ttgaacatcg cctggaacag tggtgactgc
      301 ccgagccggc gctccggagc tatcgtctcg acgatcttct cgaacggtag agctcgatga
      361 gactgcgcat ccaacaccgc gcgcttggtc ctctgaaaca agcggtgaaa gtcgtcatca
      421 tcatcgaggg ttagatcgac cagcagcgta ttgacgaaca gaccaaccac cccctcaatc
      481 tcggatcggc tccgacttcc actcggtact ccgatcacga tgcgctgctc accagacaat
      541 ctggacaaga gcgccgcaaa tgcaccgatc agcagcatga aagccgttac cccctctctt
      601 cgcgcgaggg ctcgcagtcg atcccacgtc ttggcgcgca gagtccgttg cacctgacca
      661 ccgcgtccat aggactccgc cgaccgggga tggtccgcgg gcaattgaag ggccggaggt
      721 tcgacgcaaa acccctcgcg ccagcttgcg atcaattcct cgagctctcc tgcctcgaac
      781 agtgcccgct ggaagaccgc atagtcagcg taagtcaccg gcagggcggg cagccgcacg
      841 gggtcgcaac ctgccgcgtc gagatgcctc gatattgcgc ctccatagta tgaggcgaac
      901 tcgcgcatca aaatgtccaa cgaagcacca tccgcgacga gatggtgaat cacgagtatc
      961 aggatacact cgcgttctcc ggagcgcgcg agaacgaccc gaagcaacgg gtcccgcgct
     1021 agatcgaaag gtttagtgat cgctggttgg gtgagcgcac gcaaagccgc ctccgaacgg
     1081 cgcgcgggct ctgggacgtg cagcgtgatc acctcgattt cgggctcggt cgagatatgg
     1141 cggtgagcca cgccgtcacg ctcgatgtac cgcgtccgga gcgccggatg gcgtaccgcc
     1201 agcgcgcgca aagccccaga cacccggctc gtatcgagct cacctcgaat gcggagcgcc
     1261 gccaccatgt gataggcggg gctgtccggt tccagccgtg acaggaacca aatcctttct
     1321 tccgcgtaag acaaggtcgt ttcggccccc gcggcgccgg cggggatggg caataggctc
     1381 gaatccaacc ctcgctcgcg cagtcgccgc caaaattcgc gcctcgcgcc cggcgctaac
     1441 ccaaggaagc gctccgacag acgctctgcc tcggaaggag cgtgcgtcat gacggctcca
     1501 cttccagttc actcatcagt tcgtccaacg cggcaagcgg attggtgacc gtggcgcctc
     1561 gttctagatc gatcgccgcc gcgagcgtcg ccaccgtcgt ggcttcgaac aactttcgta
     1621 aaggcagcgc cacctgaaat ctctcccgca atcgcgcggc cgccttgacg gcgagcaaag
     1681 aatggccgcc gagggcgaag aagtcgtcct ccgcgcgcag ccgctgaacg ccaagaacga
     1741 tctcccaaac ttcggccacc gcccgctctg tttcattgcc ggggagtcgt gaagccgccc
     1801 tgggtcgatc cggttccgga agtgccttgc gatcgagctt accgttggcg gagagcggca
     1861 tcgcgtcgag cggaaccaag aagctcggga ccgcatgcgc gggcagcttg aggcgcagaa
     1921 gctcgcatag ctcgtggtcg agggcgtcga cgtcgccaag acgcttggga acgtaatacc
     1981 ccgccaggaa agcttgctcg cccgtgccgc gcaatacagc acaggcctcg aggacactcg
     2041 ggtgttcgag caggcatgct tcgatttcgc ccaattctat gcgaaatcca cgcagcttga
     2101 cctggtgatc cacgcgaccg aggtactcca acgcaccgtc atctcggaac cgcgcgagat
     2161 caccagtgcg gtagagacgc gcactcggct ccggccccac tgcgtctgga acgaatcgtg
     2221 cggcggtgag gctcggccgc gcaaagtagc cgcgcgccag cccgatgccg cccagataga
     2281 gttcgcccgg gtgacctggc ggcacgggat cgaggtgcgc gtccagcacg tgcacgcgca
     2341 cattggcgat gggccgtccg atgggaactg taggcagatc gtccgaagaa caactccaat
     2401 gagtgacatc gatcgcggac tcggtaggac catatagatt gaacagattc gcgctcggca
     2461 atgcggcctt gcacgcggcc cgtagctcga cgctcaacgc ctccccactc gccacgatgc
     2521 ggcgtacgct ctcacacgtg cgcgcctcgg gggcattgag aaatgcctgc aacatcgaag
     2581 gcacgaagtg cagcgtcgtg atcccgtgtt tgacgaccag cgccgctaaa cgcgctggat
     2641 cccgatgctc gcccggtgac gcgagcacga gccgtgcgcc actgatcaat ggccagaaga
     2701 actcccatac agaaacgtcg aaactgattg gagtcttctg cagcacgacg tcgtgcgcgg
     2761 cgagcccgta ctgctgctgc atccacagca aacggttctt caagccgccg tgagtattgg
     2821 cacacccctt gggcaatccc gtggagccag aggtgtaaat cacatatgcc aggttctcgg
     2881 ggcgcactcg cgctatcggc ctcgagtcgc ccccgtcctc gacaacccac ttgaactcat
     2941 ccaggcacag ggtcggtata ttcgcgcacg ccgcgagggc agtctggagc gggcgcgtgg
     3001 tcagcaatac tcgtggccgc gcttcggcca ccatctgtat cagccgccca tcggggtagc
     3061 tcggatccag gggcaggaaa gcgcccccgg ccttgagcac cgccacgagc gcgatcacca
     3121 actccaaaca acgttcgagc ccgattgcaa ccaccgcgtc cggaccgact ccagaccgac
     3181 acaaggacat cgccagtagg tccgatcggc gatcgagctc acggtaggta atctggtcgt
     3241 tctcgaagat gacagcaacg cgatccgcgt acgtgtgggc cgcgtgtcgc agcaaatcga
     3301 ggattgaccc cggaaccccg aagtcggtct gggtcgcgtt ccacgccgcc aggcgagcct
     3361 gatcactccg actcagccgc ccgaactgcg cgagaggtcg agcgggctct cgaactgcat
     3421 ccgagagcag acgcacgtac tccgcgccga gccgctcgat ggtcgtttca tcgaataggt
     3481 cgcgtgaata caccacttct gcccgcaagg cgccgctgtc gtcttcttcg gtgtgtagag
     3541 cgagctcgaa ctgagccgag gtctttggca actcgaagcg ctcgagaccc agtccggcga
     3601 gctcgcgcag ggcggccagg tcccgtcgtt ggtggttcat caacacctga aacaacgggc
     3661 tacgagtcga gttgcgctcc acacgtaagg cttccactat ccgttcgaaa ggcacttcct
     3721 ggtgctcctg ggcttcgagt acggctcgac gcgtccgcgc gagcaccgac gcgaagctct
     3781 cttctccacc gagcgtgagc cgcagtggaa gtgtgttgag cagcggccca accagctctt
     3841 gagtatccgg atgtcgtcgg ttcgcgaccg gcattccgat gcagagctcc tgctgcccag
     3901 accagcgctg cagcaatgct gcccagcctc cgagcagcac catgaagaga gtcgcagagt
     3961 ggagttcggc caagcgacgc agtcgcccct ggagttcgag cggtatatcc acgagcaggc
     4021 tgccgcctcg gctactcgga cgcgccgggc gaacgtgatc gagaggcagg gccagcggct
     4081 ccggaattcg agcgaatgct ccgcgccagt actcgagctc ccgggccacc gatgagcttg
     4141 ccagtcgttg gcgctgccaa gccgcaaagt ccgtgtagtt tggagtctgg ttgggcaaac
     4201 atgccgatcg cccttcgacg gacgccgcgt accgttcccc gaattcctgg accaacacgt
     4261 tcatcgacca gccgtccacc acgatatgat gtgcgctcaa aacgattacg tagtcctctt
     4321 cggagagctg gatacaaaga gcccgccaca gcggcgctcg agaaagatcg aacgggcgct
     4381 cgagttctcg ggtagcgagc cgtcgggcct cgtgttcgag cgcgcgcaaa ctcgaggtgg
     4441 cggccgtgca ctgggtgaag gtcaattcgg tctcgacctg ctccagcaca cggcgttcga
     4501 cctctcccga ctctgtttcg tgggagtcga agcaggagcg caggagttcg tgccgggccg
     4561 cgagctccgt tagggcagag cgcagggcgg ctcgattcag cactccgcgt aagcgtaccg
     4621 caccccccac gacgtacgca tggctctcgg gctccagcgc agagagcatc caaagccgct
     4681 gttgcaccga gctcagcagc tttcggtcac tggtcgaagc gcggggaatc ccaacctccg
     4741 attccgagta ctcggcagtg gcgccaccga gagcacgcgc caacgcggcg atactcgggt
     4801 gttggaagag caacttggga ttgattctga gcttctgctt gcgtgctctc gcaatcacct
     4861 gcaggctcaa gatggaatca ccgcccagct cgaagaagtt gtcgtgaatt ccgatatcct
     4921 ggcgcttgag tactgcggcc cagatttcta gcagtagctg ctccaccggt gagtttgcct
     4981 gggccaagtc agaacggctg ggtccgcctt ggttcagacc gaccgccggc agccgccgca
     5041 gatcgatctt gccgtttgca gtcactggca gttcagccac ttcgatgagc tgaggcacca
     5101 atgatgccgg aagaatgcgc tcgagttgtc ggcgaacgac ctcggtcgtc tctcggatag
     5161 tcagtgctcg ttgggacccg tcgcctcccg gacgcgccgc cacgtagtag ccaaccagac
     5221 gcaatgagtc ttcttcaccg ggcgccgtca cgacgaccgc gtcccggact tcgctcagcg
     5281 agcgtagtgc cgcttggatt tctccgagtt ctactcgttg cccacgtatc ttcacttgag
     5341 agtcggcgcg acccagaaac tcgacccttc ccgcgcgcac gcacgcgcga tcaccggtcc
     5401 gatagatccg cctcccagag cctgccggat ctggaacgaa gcgcgctgcc gtgagcccgg
     5461 gttgcccctg atagcccctc gcgagggctg gtccgcccac gtagagttca cccgccactc
     5521 cctccggcag caggttgagc cccgcatcca gcacttgcac ggtcgttcct gccaatggtc
     5581 gcccgagggg gggatcgcgc tccacatctg agacttccac ggcgtgggtg agcacaccga
     5641 cggtggtctc ggtggggcca tagtgattga tgatcgcgag atcggggcga agctctcgaa
     5701 ttttgcgcag tagcgacggg gaacacgcct caccgcccaa gacgagcgtg tgcgacggca
     5761 gcacgtcgct tgggcatgca gcgtgtagga gacccgacaa gtggctcgga acgatcttca
     5821 acaccgcgac ctcgtgggtt cgcatgatcg aggcgaaagc gtctggatcg aacccgctct
     5881 cgtttgggat caggcacagg gcgcgcccag cgactagcgc tccgaatagg accgtatgtc
     5941 ccaagtcggc agcgaccgta gacgccatcg cgaacgcgcc ctgaaccggt aagctcacgc
     6001 gctcgagcaa tccggagaga tagtgggcga gcgaaccgcg actgacggat acagccttgg
     6061 ggcgccccgt cgagccggac gtatagatca agtaggcagg agcgtctcgg tgaaattcac
     6121 ggggaagcgc tccctcaccc gctcgaaccc gaacactcga atcgcgtagc gcgtcaatgt
     6181 ccaagcccgg caattcgtct tcgaccagag tccgatgtgc cgagctgaac agaatgcagc
     6241 acgctcccga atctcttaat atgaagtttc gccgctcggt cggtgtctcg ggctcgaccg
     6301 gaacgtaagc ggcgccggct ttgagtacac ccagcacacc ggcaacgaag gcagcggaac
     6361 gcggtgtgca caacgcgacc acgctttcta gaccgatctc ttgggcacgc agcgcaatcg
     6421 caatctcatc ggaccaagcg tcgagttcag cccgggtgag ggagtcatcc gccgagcaaa
     6481 tcgagatcac ctcgggcgct tcggccactc gtcggcgcca attcagcacg atgtcgtcca
     6541 acggctctcg cgacgccggt tcgctgagga gcgccgacgg accgaagatt ggtaaaccga
     6601 tcgagcctac tttcgcctcc cgaccggcgg acagggcctc ggcgactcca agcaactggc
     6661 gactgagggc ctccgccgtc acgtgatcga tccgtccgag atcgtgggag agcgcaatct
     6721 gaatcccgtc ctggaacgac acggcaaccg tgagcggata gttgttccga ccgtttcgcg
     6781 acagcagatg ttcgatgggt gcgctctcct cgagcaactt cacgcggtgc gccgcggagt
     6841 gggcacggag cacggacgag atcgggtaat tttcgatgac gagcacgctc tcgaacaacg
     6901 gcaatccctc cccaaatccg cacgtccgct ggatctcgct cagtcccacg ctcccgtgct
     6961 cccgaagcgt cgcgccaagc tcctgcagcg cgtacagcca ctcgccgacc ccgctttcgc
     7021 tcggcatcac cacgcgacac ggcagcgtat tgatgaacaa acccacgctg tgctcgatcc
     7081 ctggcacgtc cgttggtcga cccgaaaccg tcactccgaa caagacatcc tgagaacccg
     7141 tgtgtcgcgc caagacaatg gcccaggccg cttggaacaa agtggccagc gtaactcccg
     7201 caccttgagc caactcccgc agccgctgct cctgcgagca tgaaacgcgg agggagtact
     7261 ccccgtactg tccctccaag ttcggatcac ccttaccgcg cgggagagcc gtcggccctt
     7321 ctatacccgt caagtgttct cgaaagtatt cgagagactc gcctgcgtca gcttgggtga
     7381 ctcgacacac gaactccgcg tacgaggctg ctcgcgggtc cggggcctcc agattcgaat
     7441 agagctccag gagctcactc atgacttgag cgaggctcca accatccagg gcgatgtgat
     7501 gatgactcca gattagccag cccgcgctgg gcccgcgctg cagccacttg aatctgaggc
     7561 tatgcgcgcg cgcgaagtcg aatggagcct ctcgcgtctg tcggagatgc gcaccgagag
     7621 ctcggctcgc gtcagcttcg gacaactcgg agaggtcctc ttcctcccac cgcacgtccg
     7681 cggcacgcat tatcacctgc acggggccgt tctcgaactc ccaaaagtat gcagtgcgca
     7741 atgccgcgtg gcgctccgcg actcgacgga acgcgcgctg cagacgctgg ccgtcccact
     7801 cgccctcgaa acagaagccg cgctgaaaga agtacggatc ggccgcgccg cgctcgaccg
     7861 cgtgaaacag tatcccttgc tgaatcgggg ttagaggcag aatgtcctcc accgaatccg
     7921 ccgcgaacgg gagcgagctc aactcgcgtt cggacaggcg gaccgcgggg aagtcggagt
     7981 tgcgcagggt gccgctcttt cggcgcacga gctcggcgat ggcggcgcgg aagcgttcca
     8041 tgaactcctc ggccgcccgc ggctcccacg cctcgggatc gtgagagagc tcaccgatca
     8101 gccgatcccc tatgactcgg aggtcgagcg tcgccgggac cagcgaaggg ctctcggggt
     8161 cacgcagctc caccgtggag cctggctcca ccaaacggaa ccaatccgaa tctcgaaggc
     8221 ccgcgtccag gcgtcccaag tagttgaacg tgagcttcgg gttcagcgag cgagcctgcc
     8281 ccgttcccga tccgaagcgg cgccaaactc cgtagctcca cgcatgcggg gactgcgctg
     8341 caagttcttg gcgtgcgctc tcgaccgtgt cgctcaagct gccgttcggc ctcaagctca
     8401 gcggataggt tgccgtaaac caacctacgg tgcgtgagat atcgagtggc gttgcacctg
     8461 cgtcgcgccc atgactctcc aactcgaccc agagctcgtc accggcgttc tgcatcgagt
     8521 gagcgagtgc gcccagcagt agagcttgca agggaattcc cgccgctcgc gcccgacgca
     8581 ccatctcccg gtaagaagca ggaacttcga actgcagctt ggatgacggt cgatagaccc
     8641 ggtgagatgc agcctgcgtc cgatccccca gcacacttag ccaatgctcg cgctcggcgg
     8701 caaatcctgc tgaagaagcg atctgctcca gactgcggat ccaggaaccg aagctagccg
     8761 tcttgggcga cagtgcgccg ccgttcagta gaacttctag atcttcgatc aagattcgaa
     8821 acgagaccgc gtcaacgact aggtgatgaa tcgtgaggaa caccaaacga cggccgtccg
     8881 agtgcaagaa gtgaatcgcc ctgagtaagg gcccattctc gaggtcgagg ctggaatgcg
     8941 ccaaacggca ttcgcgctgc aactccgcgt cggttgcgac ctcccgccgc caaaccagag
     9001 ccgtcggctc ctcgtgagcg tagtgctggt agggttcagc gacgccaacc tccgcaaaac
     9061 agaagcggag gcgtagtgca tcatggtgcg caaccagttt ctccaacgcc gtggtcaagc
     9121 ccgctgtgct tacttcgcgc gtcagctcca gtaacaccgc ttgattgaag tgttcgggtc
     9181 tcgagggcct taggcacagg aactcggact gaatcggcga cagtggtaac cgcccctcag
     9241 gaagagcctc aacctgctcg ttgccaacct gctggatggc ccgagcgagt tgttcgatag
     9301 ttggattggc gaaaagatcg cgaggagtga acaccagccc actttggcgg gcccgcccga
     9361 cgagctgaat ggctaatacg gaatcgcctc ccagttcgaa gaagtgatcg gcgacgccga
     9421 cctcttcgac ttgcagcaac ttctgccaca actcgactaa cttctcttga acggagttgg
     9481 tcgccggcac gagctcgcgt cgaacggccg ctggctcagg cagcgcagcc cgatcgagct
     9541 tgccattcgg accagtcggt aacgccgaaa gacataggat gcgcccggga accatgtatt
     9601 ccggcacgcg actcgccatc gagtctttga gttgttgttc caacctagcc gtgttctcca
     9661 gcacagccgt gttctccagc accacgtagc ccacgagctg cggatgcgaa cccaacctgc
     9721 gcagggcggc cgcggcggcc tttacgccgc gtagcgcggc gagctccgcc tcgacctcgg
     9781 cgagctcgat acgatgtcca cgtaacttga tctgatgatc gacgcgaccg acgtactcga
     9841 acacgccgtc cgggcgtcgt cgggcgcgat cccccgtcct atacaggcgc gctcctggcg
     9901 ccgcgctgaa cggatcggga acgaagcggg cggccgtgaa tcccggttgt gcgtgatagc
     9961 ctcgggccag gccttcccca cccaggtaaa gctccccgac ggtgccctcc ggtacgggct
    10021 ggagatcaga gtccagcacc caagccgatc gacggcccac gactcgcccg atgggggcga
    10081 gcggggtgcc ggcgggtcca gcgtctgcct gccagagcat gggtgtaatg accgcttcgg
    10141 tcggaccata accgttgatg atcttctctg gacgaagccc cgctcggatc aattcgatgc
    10201 tgctcgtttg gacggcttcc ccacccacgg tgcacacgcg cacgcgaccg agcgcgcctc
    10261 cgctcagacc aaaccgggcc aactcgacca catagttggt gggtgggtaa acgatactaa
    10321 tctgctcacg ggcgacgatc tccgcgattt gcgcgccgga ccaagcgcct tggctcagca
    10381 cgacgccacc gccgaacacg agcgggacga cccactgctc cacaccggcg tcgaaactca
    10441 tggaggccat gtgcaaggcc cgatccgtcc tccgcatttc gtaaagtcgc gctgccgctg
    10501 cgcaatgcat ggccagcgcg ccctgactca cggacactcc cttgggggta cccgtagagc
    10561 ccgaggtata aatcaggtac gcggcttgat cgggtaaact cggcgtgagt tctcggagca
    10621 attcggccga tccatcgata ctggcgagtt cgatgacagt gcccgtaaac ccgccgtcgg
    10681 tgaggttcga gctggattga tccgcgagca acactctggc cccgcagtcc gcgatcatcc
    10741 attgaattcg agaaatcgga tactctggat cgatgggcac atacgcgcct cctaccatcc
    10801 acacaccgag catcgccgcg atgaggctcg cgctccgtga ggcaaagatg gccactctct
    10861 gttcgcgacc cacacccagc cgtgccaacg cggccgcaat ccggcgggcc cgcgcctcga
    10921 gctcggcgta gctcacccgc tcttcacccg cgatcaccgc ggtgcagtcc gcctggtgcc
    10981 caacagtggt caagaactcg tccagcagag caggcgttgc ttcgccctcg ggatcgacct
    11041 ctcggaccgc ctgctcgcac caactcgcaa gctgcatatc cgagaccaag ccgatctcac
    11101 caattgcccg ctctggagca tgcaccatgc gagccaataa cagcaggtag gcgctaaccc
    11161 aagcgcggat cgtagactct tcgaacaact cccgcgcgta accaaagcga ccaaagcatc
    11221 gtcct
//
LOCUS       DASTKU010000205         8227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_40447,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000205 DASTKU010000000
VERSION     DASTKU010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40447"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..802)
                     /locus_tag="VFQ61_07770"
     CDS             complement(83..802)
                     /locus_tag="VFQ61_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9954385.1"
                     /translation="MGAVAKTVVIILLVMSMYAIAIALERFLTFKRSRERSLAYVAAL
                     QPLISAPGRLHEAARLGPQWKDAPLARILGVGLEEFTQGVEQLGPAAADSAEIELLVH
                     GVGRSMERAKKREMAGLGKGLPVLATISSSAPFVGLFGTVFGIITAFQQMADPSKGGG
                     GGLATVSAGISEALLTTAVGLAVAIASVWFYNFFTIRLDELGVIIDDATGELSDRLLH
                     SNRGLRRNGAVGSTSVAPVSA"
     gene            complement(863..1600)
                     /locus_tag="VFQ61_07775"
     CDS             complement(863..1600)
                     /locus_tag="VFQ61_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9954386.1"
                     /translation="MAFEGILAPPDKRPSRWRRIMVTVSLALHGIGLVVGVIVSLWKV
                     DELPMPAIHVTLANAPPPPPPPPPPPKKSASTKPKTKPTEAKPSVLTAPKEIPKEEPK
                     PEPEAAKDDDDDKGVEGGVEGGVAGGVLGGVVGAAPPPPPPKPVAPTNVPLQIARQQL
                     LINPDVDPYKVRMPPALARSGATSDSVVRICVSKQGNVTGVSIVKPGLGVIDQQLPTV
                     IRRWRYRPIMKNGEPIEFCYVMRYELH"
     gene            complement(1604..2563)
                     /locus_tag="VFQ61_07780"
     CDS             complement(1604..2563)
                     /locus_tag="VFQ61_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954387.1"
                     /translation="MKFNSTLVCSIIAIGALSGCSELRARSTAREGNEQYRDGNYAAA
                     AQKYEAAQQLHPDIPVILFNRGLACRQLMVPGAKTKESEQGVNCALDAFKRLQAVNPK
                     DERAEQLYIQTLFDADRFETLAAMYEKRLQENPKDSSSINGLIQVYSRWNKPEEALRW
                     TMRRADSIPSDAEAQYSVGVYIWNQLFMRGGGVDKQSFDPRPDPNNPKVLKIPPAFAS
                     TDVAGPERIKLADQAIQYLEKALTIRPSYKDAMVYLNLVYRQKAFAYFDQPQEWQKLI
                     DTAETWRKKATEMDVPPNGAASASSATPNAPAASAAPAPAKSH"
     gene            4617..5279
                     /locus_tag="VFQ61_07785"
     CDS             4617..5279
                     /locus_tag="VFQ61_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954388.1"
                     /translation="MNFIRVAVVALSTSVSTLLACSASDTVISVNVRANENVGAVERL
                     TLTISQDGQSPVTRTLDDSTKLNTRTGKIFNYQTGVVEQIPNPETDPEKLKEKPTIDK
                     TGPVLKTDFFERFILPDGWKEGKATITGKVALVEAQADGSKELDTKMPVEVSVIEEAA
                     VAAYIDFERHFPEATGGTTGTGGATATGGSTGETGGTTGTGGSTATGGTTSDAGAGGA
                     GS"
     gene            6080..7729
                     /locus_tag="VFQ61_07790"
     CDS             6080..7729
                     /locus_tag="VFQ61_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9954389.1"
                     /translation="MASIQVQDVTKAYGQKKLFQEVTVNFSSGRRYGITGPNGAGKST
                     FLKILAGDIEPDTGYVQKPERTSVLRQDQFAFESWRVLDVVIMGNARLWAAMEEKASL
                     LGNPNLTDDDGVRLAELEGVIAEEDGYTAESDAGELLSGLGIPESDHEKVMSELPGGR
                     KLRVLLAQALFGNPDALLLDEPTNNLDIETIRWLERFLQNYKGVLLTISHDRRFLNEI
                     CTHVADIDYETIITYTGGYDDMIAAKGQVRGKIEQENAERKKKIEQLQDFVARFAAGT
                     RASQVQSRKKQIDRLSLNDLKRSNIERPFIQFLVKKPSSKQTLVIEGLTKRWPELTVC
                     EGFRATVTKGEKVAIIGRSGVGKTTLCKMMMGEVPPDEGVVEWGTQTSLGYLAQDHRS
                     GIMPNTTVSQWLHSFDTSASLQDVRGLLGRMLFKGEEGSKPTEALSGGEAVRLIFSKL
                     MLTKDNVLILDEPTNHLDLESIIALGDALAKYEGTAFVVTHDRDLVATFATRLFAFTH
                     EGLIDWQGSYTDYMERFGEDGQLHSPSGGGEAGRSKGRSGR"
     gene            7798..8058
                     /locus_tag="VFQ61_07795"
     CDS             7798..8058
                     /locus_tag="VFQ61_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954390.1"
                     /translation="MSRSTLAPRTLRLISTARQNPEFAARLASELPEPSRRLLMTELA
                     RPEPARAPGAKGLAKCKPSPYAPENGLTAQLSLFAQRASRVR"
BASE COUNT         1508 a         2470 c         2631 g         1618 t
ORIGIN      
        1 atcaccttgc ggggctcacg ctcgtcgtga tgcttgagag cgcggagcgc ccgcagacga
       61 tcgaatttgc caggcgcgcg tttcacgcag acaccggcgc aaccgacgtg ctaccgactg
      121 cgccgttgcg gcgcaagcca cggttgctgt gaaggaggcg gtccgacaac tcgcccgtgg
      181 cgtcatcgat gatcacgccg agctcgtcca agcgaatggt gaagaagttg tagaaccaca
      241 ccgacgcgat ggccacggcc agaccgacgg cagtggtcaa cagagcttcc gagataccag
      301 ccgacacggt cgccagaccg ccgccgccgc ccttggaagg gtcggccatc tgctggaagg
      361 cggtgatgat accgaacacc gttccgaaca gaccgacgaa cggcgcggaa gaggaaatcg
      421 tggcgagcac gggcaagcct ttgcccaggc cagccatctc gcgcttcttg gcgcgctcca
      481 tcgagcggcc gacgccgtgg acgaggagct cgatctcggc cgaatctgcc gcggcaggcc
      541 cgagttgctc cacgccctgg gtgaactctt ccaggccaac gccgaggata cgcgccagcg
      601 gagcgtcttt ccattgcggg ccaagacggg ctgcctcgtg caggcgaccc ggcgcggaaa
      661 tgagaggttg caaggctgct acgtaagcga gtgaacgctc gcggctgcgc ttgaacgtga
      721 ggaaacgttc cagcgcgatc gcgatcgcat acatagacat cacgagcagg atgatgacga
      781 cggtcttcgc gaccgcaccc atctggtgcc acaaaccgag taatgagaag tccatcttcg
      841 ttgtctcttt tttagatcct cgtcagtgaa gctcgtaacg catcacgtag cagaattcga
      901 tcggctcccc attcttcatg atggggcggt agcgccagcg tcggatcacc gtcggcagct
      961 gttggtcgat cacaccaagg cccggcttca cgatggatac gccggtgacg ttgccttgtt
     1021 tcgatacgca gatgcgcacc actgagtcgc tggttgcgcc ggagcgcgcc aacgcggggg
     1081 gcatgcgaac cttgtaggga tccacgtcag gattgatgag cagctgttgt cgcgcgatct
     1141 gcagtggcac gttcgtcggt gccaccggct tgggcggggg cggcggtgga gccgcaccca
     1201 cgacgccacc cagcacgcct ccggcaacgc cgccttcgac accaccttcg acgcctttat
     1261 cgtcgtcgtc atccttggca gcctcgggtt ccggcttggg ttcctctttg ggaatctcct
     1321 tcggtgccgt gagaacgctg ggctttgcct cggtgggctt ggtcttcggc ttcgtgctgg
     1381 cagacttctt gggcggcgga ggcggaggag gtggtggcgg tggcgcgttg gccaacgtca
     1441 cgtgaatggc tggcatgggc agctcatcca ccttccacaa cgacacgatc acacccacga
     1501 cgagaccgat cccgtgcagc gcgagcgaca cggtgaccat gatccgacgc cagcgggacg
     1561 gccgcttatc tgggggagcg agaatacctt cgaaagccat gactcagtgg ctcttagcgg
     1621 gcgccggcgc tgcactggcc gccggggcat tgggggtagc agagctggcg ctagcagcgc
     1681 cgtttggcgg cacgtccatc tcggtggcct tcttgcgcca cgtttccgca gtgtctatca
     1741 acttctgcca ctcctgcggt tgatcgaaat aggcgaacgc cttctgccga taaacgaggt
     1801 tcaagtagac catcgcgtcc ttgtacgaag gacgaatcgt gagcgccttc tccaggtact
     1861 ggatcgcctg gtccgcaagc ttgatgcgtt cgggaccggc gacgtccgtc gacgcaaacg
     1921 ccggcggaat cttgagtacc ttggggttat tcggatcggg acgagggtcg aatgattgct
     1981 tatcgactcc gccgccgcgc atgaacagct gattccagat gtacaccccg accgagtact
     2041 gggcctcggc gtctgacgga atcgagtcgg cgcgccgcat cgtccagcgt agcgcttctt
     2101 ccggcttgtt ccaccgcgaa tagacctgga tcagaccgtt gatcgagctg ctgtctttcg
     2161 ggttttcttg cagccgcttt tcgtacattg cggccaaggt ttcgaagcga tcggcgtcga
     2221 acaacgtttg gatatatagc tgctcggcgc gttcgtcctt cggattcacg gcttgcagac
     2281 gcttgaaggc gtcgagcgcg cagtttacgc cctgctcgct ctctttcgtc ttcgcgcccg
     2341 ggaccatcaa ctggcgacag gccaggccgc ggttgaaaag tatgaccggg atgtccggat
     2401 gcagctgctg tgctgcttcg tacttttggg cggcagctgc atagttgccg tcgcgatact
     2461 gttcgttgcc ctcacgtgct gtgcttcgcg ctcgcagctc ggagcagccg gaaagggcac
     2521 cgatcgcgat gatgctgcac accagggtgg agttaaactt catgagcgcc cgtgacaaaa
     2581 cggcgtgggc aatccacgcc cgtagcgaat gcggaccgtc gcaacgcgcg gggctttcca
     2641 tacacaattc acgtggccgt gcgcaaggga ggccaacttt ttgattaccc attttccttt
     2701 gctcgggtcg ggagtcgcga cgcactgcag cggcgcgggt tgggttgccg cgttctgcct
     2761 atagggcgcg catgggaacg gcctatgagt agtgcctcgg aaccgcctat aaagggtgcc
     2821 tcggagaagg ctgtgaagag tgccttggaa aggcccatca gaagtgcccg cgagaggcct
     2881 atcaaaagtg cctatcaaag gtgcctttgg gaacgcgtac gaaaagctcg gggggcccgc
     2941 gttcagctcc tggctcgaag ccagggtggc tgggggattg gactgcggag agtctggact
     3001 tggcgggccg aagccgccag cgccaagcgc ggattttttc ttcactcgtc ggagcggcgt
     3061 ttcgcattgg gaatcccaat tagaagcggc atggcgctcg tcaccagatt cccgacttga
     3121 atgcctatat ggagggttat gcacagcgtg agctgacgac ttccaaatgc tcacaaagca
     3181 cggagtgctt cgcattttgg ctagcgcagc gcgctgtggt ttggccatgt tttccggcgc
     3241 tggcctcgag ctcacgactt ccgcaatcga gcatagtttt ccgcgcgaaa tcacgctcac
     3301 ttcgctcgcc tctggctccc gatgagagcg ccgccgcccg cctcgcgccc cacactcgac
     3361 gcctcgaata tcgggcactt ccgtaattgt cggaggcggc tcgattcggt gactgtccgg
     3421 ggtgattgct gtggcggtgg gcaatgttct gatctcgatt gacgtgctga tctcgattca
     3481 gcgcgagttg cggctgtaag tgcgatgtta gaggggggct ggctgcgttt ccttcgctcg
     3541 ctgatgtgac cggagtctga cgtctggcag ctgcgttctt tggcggctgt ctttatgccg
     3601 cgaccgtgtt tctccggtag tcatgctcct catgaggcca tgctcgcctc gcgaccgcca
     3661 ccgtcccacg atcgcgttct ctagtgactt gtttcattcg cggtcctgtt tcgcggcgtg
     3721 cttctcagtg gcttcagccg gcgaaatctg tctgtctccg ctcaggccca cctgtttgtc
     3781 cttctcgtcc gccgatgggt ttgccgggcc gcggctgagt ccgcatggga gacaagggtt
     3841 ctgggctcca cctcttctgg tcacgggtct accctgctct ttgtcgcgtc agcccacttt
     3901 cagttccaca atcgtcatga tcctggcagt cctcggatga atttggcttg gggcacggcc
     3961 gagcggggac ggtgtgcggg ctgtccccaa cgctgcaaag gagccttgcc gggataggtg
     4021 ggcccaaggg agtagttcgt aagaaagcga gctggtgagc aggggagtca gcacgcttcg
     4081 gtaagcgccg ccctcgagca cgcgcgcggc tgcccctacg gcgcgcgatg ctccaatcat
     4141 ctcgtcggaa gggaacacag catagggcga tgctgcgcag caacggtgcg aagcgccgtg
     4201 ctcggcgcgc gaaccctcgt tctctcgggc agcagaggag cccgcgcggt agtggcattc
     4261 tctcgctgct cggtgggcat cacgaagccg ccgaaacggc ggtcgagacc tggagaacgc
     4321 gccctgaggc cgctcgcccg gttgccccgc gaccggcctc ttcgaggcag ccttctgcgc
     4381 tgcgtcgatg gcgatcgagc ggtgcccgcg atgctctcga aacggcggga aagcggttca
     4441 attgcgtcgg cgaggtcgcg cactcccgag gggcgcgcgg tttccagacc cggtcaacat
     4501 ttgctggtgg cgctgcgtca gttacgagag ctttggaagg tcagacgacc caatcttaga
     4561 aagaagtgtg aacgactgtt gctgttctcg gtccacaccc actaagatac gccgccatga
     4621 acttcatccg tgttgccgta gtcgccctct cgacgtcggt atccacgctg ttggcttgct
     4681 cagcctccga caccgttatc tcggtcaatg tccgagctaa cgaaaacgtc ggtgccgtgg
     4741 aacgactcac cctcaccatc tcgcaagatg gccagagccc ggtcaccaga acgctggatg
     4801 actcgaccaa gctcaatacc cgcactggga agatcttcaa ctaccagacc ggggtcgtgg
     4861 agcagatccc gaaccccgag accgaccccg aaaagttgaa ggagaagccc acgatcgaca
     4921 agaccggtcc cgttctgaag acggacttct tcgagcgctt cattcttccc gatggctgga
     4981 aggaaggcaa agccaccatc acgggtaagg ttgccctggt cgaagcccaa gctgacggct
     5041 cgaaagagct ggacacgaag atgccggtcg aggtgagtgt catcgaagag gccgcggtcg
     5101 cggcttacat cgatttcgag cgtcacttcc cggaggctac gggcggcacg accggcaccg
     5161 gcggcgccac ggccactggc ggtagcacgg gcgagacggg cggcaccacg ggcactggcg
     5221 gcagcacggc taccggcggt accacgagtg atgctggcgc tggcggcgct ggcagctgat
     5281 cgaaactgac gggaagcgcc ccggagtttg cggccggctt cgcattagct gccggtaaat
     5341 tccgggtttt cgctttcccg tgcgctcaat cagattgggc ccgcgagtaa tatcgcggcc
     5401 cgagacaaag cgggcacgac gcaaccagtt tgcgtgcccg ctttgtctat tttgtggtgg
     5461 ggcttcggcc tttagcttcg ctccccggga aagctggacg acgagtgctt cctgatgggc
     5521 caggttgcaa cgagcttccc ttgcgaagag cgagggccga ccggagtgcg tcatcggtta
     5581 ggtggttgga tgtccgccaa tgttggcaat ctccaacaag ccggcaatat actcactccg
     5641 aatgagcgct tccgtgtggc gatatgcata gatgcgaact ccagggtcga tgttgcgagg
     5701 acccgattcc ggtgttgctc gtgttttgaa gtcatagtgt gttgagatac ggtatttgcc
     5761 acggcgtcgg gctagtcact taaagagtgc tcgcagtcga ggacgatgcc ttgcgcgcgc
     5821 gaaggccggt gggaacgatg cggctcgtgg tccggtgtgc gacgttgcgt cacgtgggct
     5881 cctttgccca tcccgcgggt atgcgaccag cgagcttggg gggatgcgcg acagcgccgt
     5941 gcaaccgagc ttcccggttg agtgtcccct cgcgggctgc ggcgcttccc ggaggttttg
     6001 accctacatc ctgtttttta agccgattcg gtcacgaggc tgcgtggcgg cgaaaacctg
     6061 ctaaacgccc gcccctccca tggcatccat tcaggttcag gacgtcacca aagcctacgg
     6121 tcagaagaag ctcttccaag aggtgaccgt caatttcagc tccgggcggc gttatggcat
     6181 caccggcccg aatggcgctg gcaagtccac tttcctgaag atcctggcgg gagacatcga
     6241 gccggacacc ggttacgtgc agaagccgga gcgtaccagc gttctgcgcc aggatcagtt
     6301 cgcgttcgaa tcttggcggg tgctcgatgt cgtgatcatg ggcaacgcgc ggctttgggc
     6361 ggcgatggag gagaaggcgt cactgctcgg caaccctaac ctgaccgatg atgacggggt
     6421 gcgcttggcg gagctcgaag gggtgatcgc cgaagaggac ggttatacgg cggagtccga
     6481 cgccggagag ctgctgtcgg gtcttggaat tcccgagtcg gatcacgaaa aggtgatgag
     6541 cgagctgccg ggcgggcgaa agctgcgtgt gttgctggcg caggccctgt tcgggaaccc
     6601 ggatgcgctg ctgctcgatg agcccacgaa caacctcgat atcgagacga tccggtggct
     6661 cgagcgcttc ctacagaact acaagggcgt gctgctcacg atctcgcacg atcgtcggtt
     6721 cctgaacgag atctgcacgc acgtggcaga catcgactac gaaacgatca tcacctacac
     6781 cggcggttac gacgatatga tcgccgccaa gggccaggtg cgcgggaaaa tcgagcaaga
     6841 gaacgcggag cgtaagaaga agatcgagca gctccaagac ttcgtggctc gattcgccgc
     6901 gggcacccgc gcctcccagg tgcaaagtcg caagaagcag atcgatcgac tgtctttgaa
     6961 tgacctcaag cgttccaata tcgagcgccc gttcatccag ttcttggtca agaagccctc
     7021 gagcaagcag acgttggtga tcgaagggct gaccaagcgc tggcccgagc tgaccgtctg
     7081 cgagggcttc cgagctaccg tcaccaaagg ggaaaaggtg gccatcatcg gtcgcagcgg
     7141 cgtcggcaaa actacgctgt gtaagatgat gatgggcgag gttccgccgg atgaaggcgt
     7201 ggtggagtgg gggacccaaa ccagcctcgg ttatctcgcg caggaccatc gctcgggcat
     7261 catgccgaac acgacggtgt cgcaatggct gcattccttc gatacgtcgg cttcgctaca
     7321 agatgttcgc gggctgctcg ggcgcatgct cttcaaaggc gaagagggct ccaagccgac
     7381 cgaggctctg tcgggcggcg aagcggtacg gctgattttc tcgaagctca tgctgaccaa
     7441 ggacaatgtg ctgatcctgg atgagccgac caatcacttg gatctcgagt cgatcatcgc
     7501 gttgggtgac gcgctggcga agtacgaggg caccgccttc gtagtgacgc acgatcgaga
     7561 tttggtcgcc accttcgcga cgcgtctgtt cgcattcacg catgaagggc tgattgactg
     7621 gcagggctcc tataccgatt acatggagcg cttcggagaa gacggtcagc tgcactcgcc
     7681 cagtggcggc ggggaagcgg ggcgctccaa gggccgctcg ggccgttgaa cgagcgttcc
     7741 ggaggcccgc tcgcagaaac ttggcccacc tgcgcacggt tttgtatgtg cgccgccatg
     7801 tcccgctcca cgctcgcgcc gcgcaccctg cgcctgattt ccacggcccg tcaaaatccg
     7861 gagttcgcgg cgcgcctggc gtcggagctt ccggagccct cgcgtcggct gctgatgacc
     7921 gagttggctc gcccggagcc tgcgcgagca cccggtgcca agggcctcgc caagtgcaag
     7981 ccctcgccgt acgcgccgga gaacggcctc acggcgcagc tttcgctttt cgcccagcgc
     8041 gccagccgcg tgcgctgagc tccccgaacc gagcacctac aaagcgagag agcccgaaga
     8101 atcgggctct ctcctggcgc gcggctcgtt gtagcgagcc gcgccaatcg gcgagctact
     8161 gccgcagcat cagctgtgag cgctccgggc cgaccgatac gaagctgatg cgcttaccca
     8221 cgagctc
//
LOCUS       DASTKU010000206         6356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1154989, whole genome shotgun sequence.
ACCESSION   DASTKU010000206 DASTKU010000000
VERSION     DASTKU010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154989"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /locus_tag="VFQ61_07800"
     CDS             <1..1458
                     /locus_tag="VFQ61_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954391.1"
                     /translation="ECGQTSCYRAGGQLRSLGARLRIHYIPGNHDRLVNVFPSLRARV
                     RQLLNLAGEPRSPFAHVLEWPAYGVRVRHGHEYDKANFPIAFDGLHEPTEEHYAAPVL
                     GDFVTVELATRLALAFRGYYARELRQAGPEGDAFRRIYLALTEFDDVRPASAFPTYVA
                     KEVGRGSDWVFARLRPAIEDTLVAATQSEFFLREATRLGFAQFFEGAAGFQLRLAIAN
                     ASPHQLSQLIANMSRFQDAPGPAPADIAALESGLLEGQPLCVIAGHTHKPGQVAIPGR
                     PEHAACFVDSGTWRTRMEQGVAETFGRFRSYTIVFCYRAEEWASSRVRTFETWTGHLV
                     GEAFGSYSGRDVSDRFGASTPSQRRLQFTHVDVVGIDEGETAQGAELELDFGVDDRAL
                     ELRRNRVYDGERIQLDEAFSVWADPALDGEVWGWGKERDLGNTIVDRDDPLSFAVAYL
                     ERRSDGSFADLKAPGELHLVRLPKNHLVLGYRWVD"
     gene            complement(1479..2267)
                     /locus_tag="VFQ61_07805"
     CDS             complement(1479..2267)
                     /locus_tag="VFQ61_07805"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9954392.1"
                     /translation="MSESESGFEVIVLGAGDTFSEHLHPTALLLEAQGFRLAIDCPDS
                     YRAILRHASVRSNRPLSIADIDCVLITHVHGDHMNGLEGFAFYKHFVEHKRVRLVASR
                     EVRQGLWEQRLKLSMGTLWDGARFHSLEFEDYFDYVPLDWHEPLELGPFRIRARRTVH
                     HVPTSALHVECQGRAFAYSSDTAFDPELIRFLEPANLIFHETNYGPAHTPYESLLTLP
                     AELRERVRLIHYSDLFVASVLEARAAASGPPPIQLAHEAEIWRP"
     gene            complement(2260..2877)
                     /gene="coaD"
                     /locus_tag="VFQ61_07810"
     CDS             complement(2260..2877)
                     /gene="coaD"
                     /locus_tag="VFQ61_07810"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET9954393.1"
                     /translation="MQPHIAVYAGTFDPVTRGHLSVIERASRLFERLWVVVAIHPHKT
                     PWFTADERVELLREVCARFRNVSCAHARGYVVDFARAHGARYLVRGVRSTPDVEGELA
                     LASANRRLAPEIETVFVPAHPELSEVSSSRLKQFAEQGQDISEYCPPLVARRVFDICN
                     LRQHDASGAWLDGSAPPLAAAPVAARAAVRESIALPENTERNRHV"
     gene            complement(2883..4193)
                     /locus_tag="VFQ61_07815"
     CDS             complement(2883..4193)
                     /locus_tag="VFQ61_07815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9954394.1"
                     /translation="MRSTPAGADAGVAGSVGRLQALGKLGLLGCLYFSQGLPFGFFTQ
                     ALPVLLRKQGLSLGAIGLSSLLAAPWALKFLWAPLVDHHGSVRFGRRKSWIVPLQLAA
                     CVTLVALAFTLDLSPGAGEARRPLQGLFMAVFVLNLLAATQDVATDGLAVDLLSIRER
                     GLANGLQVAGYRVGMIIGGGVLLVLYERLQVRGTFLVMAALTALATLPVLFKREPTPL
                     VRAVSGRPSWTAFVARPGGLRIISLLLTYKAGDAFATGMLRPWLSDLGLSLADVGWLL
                     GSVGFIAGLLGALFGGLLLNFLTRRRALVTFGILQVATLFAYIALARQPAGAVVNYPL
                     LYVACAAEHFTSGMATAALFTCMMDWSEPSRAATDYTVQASTVVIASGAASALAGLSA
                     QHLGYFAHFSLATLLAASSLIWVFRAFPLGTAAPASGQAGRASE"
     gene            complement(4190..4879)
                     /locus_tag="VFQ61_07820"
     CDS             complement(4190..4879)
                     /locus_tag="VFQ61_07820"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9954395.1"
                     /translation="MPRPTNTEQRRAEITRAFVKVMAERGYDGASIGDIAREAALTPG
                     LVHYHFSNKRAILLAALEQLVARHVERLDAALATHGADPTRELAEFLDMHLGTGAKAD
                     PEALRCWVLLSGEALRDEEVRSAFERSLAEILKRLQVIIERGRAVSVFRCESAMAAAA
                     ALFALIQGYFVLAATARETIPRGSAAACARQMAQGLLSVTFPIRVGTPVSRNARAASV
                     ARKARTRKVRS"
     gene            5105..6265
                     /locus_tag="VFQ61_07825"
     CDS             5105..6265
                     /locus_tag="VFQ61_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HET9954396.1"
                     /translation="MKPPPHRTVEPRFFATAAKGTEPLLRDELRELGLPLVRADRGGV
                     QFGAEPRHAFVACLWSRIALRILSPIAEFPCPDEDALYDGVREVDWSRWIDARRTLLV
                     RAACRSSRLTHTQYISQRSKDAIVDQLRDRTGSRPDVARESPDLLLFVHLVKDRATVY
                     LDYGGDSLHEHGFRHQSGVAPLRETLAAALVRFSGWDRQSPLTDPMCGSGTLLIEAGL
                     WAAQRAPGISRQFGFERWLDFDAERQRELAQLRQAARETARQDTPVTLFGSDSDASVL
                     ELARANAARVGLRLELRAAPLRVVNPQSEQGTLVTNPPYGQRLAEGPEQRRELENVLR
                     RFAGQQRALIVPQDYPSTLRPDRYLAVFNGNLACEFRRYEARRETAHRSSES"
BASE COUNT         1219 a         2089 c         2028 g         1020 t
ORIGIN      
        1 gagtgcgggc aaacctcgtg ctatcgagcg ggcgggcaac tacgttcgct aggcgctcgg
       61 ctgcgtatcc actacatacc gggcaatcac gaccgtctcg tcaacgtctt cccgagcttg
      121 cgagcgcgcg tgcgtcagct actgaatttg gcgggcgagc cgaggagtcc gttcgcgcac
      181 gtgttggaat ggcccgcgta cggcgtgcgc gtgcggcacg ggcacgagta cgacaaagcc
      241 aacttcccga tcgctttcga cggcttgcac gagccaaccg aagagcacta cgccgctccc
      301 gtgctcggtg acttcgtgac ggtggagttg gccactcgct tggccttggc tttccgcggc
      361 tactacgccc gcgagctccg ccaagcaggc ccggaggggg acgcgttccg acgcatctat
      421 ctcgccctca ccgaattcga cgatgtacgt cccgcctccg cttttccgac ctatgtcgcc
      481 aaagaagtgg gccgcgggag tgattgggta ttcgcgcggc taaggcccgc gatcgaggac
      541 acgctggttg cggccacgca aagcgagttc ttcttacgag aggcaacgag actcgggttc
      601 gcgcagttct tcgagggagc cgcagggttc cagctcaggc tagcgatcgc gaacgcgagc
      661 ccccatcagc tgagccagct gatcgccaac atgagccgct ttcaagacgc gccgggcccc
      721 gcgcccgccg acatcgcggc gctcgagagt ggcttactcg agggtcaacc gctgtgcgtg
      781 atcgcaggtc acacgcacaa gccgggtcaa gttgcgatcc ccggccgacc cgagcacgcg
      841 gcgtgcttcg tggactcggg cacgtggcgt acgcggatgg aacagggcgt ggccgagacg
      901 ttcgggcggt ttcgctcgta caccatcgtg ttctgttatc gagccgaaga gtgggcgtct
      961 tcgcgcgtgc gcactttcga gacttggacg gggcacttgg tcggagaggc attcggcagc
     1021 tactccggcc gggacgtgag cgaccgcttc ggggcgagca cacccagcca gcggcgactg
     1081 cagttcacgc acgtggatgt ggttggaatc gacgaaggag agaccgcaca gggggctgaa
     1141 ctcgagctcg acttcggcgt agacgatcga gctctcgagt tgcgccgaaa ccgagtgtac
     1201 gacggagagc gcatccagct cgacgaagcc ttcagcgtat gggccgatcc ggcgctcgac
     1261 ggcgaagtct ggggctgggg caaagagcgc gatttgggca acaccatagt cgatcgagat
     1321 gaccccctct cgttcgcggt cgcataccta gagcggcgct cggacggcag cttcgccgat
     1381 ctgaaggctc ccggtgagct ccacctggtg cgcctcccga agaaccacct cgtcttgggt
     1441 tatcgctggg tggactagcc acgacggggc agatttggtc atggccgcca aatctccgct
     1501 tcgtgcgcca gttgaatggg cggagggccg ctcgcggcgg cgcgcgcctc caagacgctc
     1561 gccacgaaga gatccgagta gtggatgagg cgcacccgct ctcgcagctc cgcgggaagc
     1621 gtgagcagcg actcataagg agtgtgggcc ggcccgtagt tggtctcgtg aaagatcaag
     1681 ttcgccggtt cgagaaaccg aatcaactcc gggtcgaacg ccgtgtcact ggagtaggca
     1741 aacgcccgcc cctgacactc cacgtggagc gcgctggtcg gcacgtggtg tacggtacgc
     1801 cgcgcgcgaa tgcgaaatgg ccccaactcc aggggctcgt gccagtcgag cggcacgtag
     1861 tcgaagtagt cctcgaattc taggctgtgg aagcgagccc catcccagag cgtgcccata
     1921 ctcagtttca aacgttgctc ccacagccct tgcctgacct cccggctcgc gacgagccgt
     1981 acgcgcttgt gctcgacgaa atgcttgtag aacgcgaagc cttcgagtcc attcatatga
     2041 tcgccgtgca cgtgggtaat gagcacgcag tcgatatcgg ctatcgacag gggccgatta
     2101 gagcgcacgg acgcgtgacg cagaatggcc ctataactat ctgggcaatc gatagccaag
     2161 cggaagcctt gcgcctcgag caacagcgcc gtcggatgaa ggtgctcgga gaaagtgtcc
     2221 cccgccccga gtacgatcac ttcgaatccc gattctgatt cagacatgac ggttccgctc
     2281 cgtgttttca ggaagggcga ttgactcgcg cacggctgcg cgggccgcaa ccggcgcagc
     2341 cgccaggggc ggcgcactgc cgtcgagcca cgctccgctc gcgtcgtgtt gccgcaagtt
     2401 gcagatatcg aacacacgcc gggcgacgag cggcggacaa tattcggaga tgtcttgacc
     2461 ctgctccgcg aactgcttca ggcggctaga gctcacctcc gagagctccg gatgcgccgg
     2521 gacgaacacc gtctcgatct cgggtgccaa gcggcgattg gcggatgcca aggcgagctc
     2581 gccttcgacg tcaggggtgc tccgcacgcc ccgcacgagg tagcgggcgc cgtgagcccg
     2641 cgcgaagtcg accacgtaac cacgtgcgtg agcgcagctc acgttgcgaa aacgggcgca
     2701 gacctcgcgc agcagctcga cgcgctcatc cgcggtgaac caaggcgttt tgtgaggatg
     2761 aatggcgacc accacccaga gcctctcgaa caagcgtgag gcgcgctcga tgaccgacaa
     2821 gtggcctcgc gtaacggggt cgaacgtacc ggcgtaaacg gcaatatgag gctgcatgtt
     2881 ggttactccg aagcccggcc cgcttgcccg gaggccggcg ctgcggtccc gagagggaaa
     2941 gcccgaaaca cccagatcaa gctcgaggct gctagcaaag tcgccagcga gaagtgcgca
     3001 aagtagccca ggtgctgtgc gctcaagccg gccaaggccg aagccgcgcc cgaagcgatc
     3061 acgaccgtac tggcttgtac ggtgtagtcg gtggccgcgc ggctcggctc actccaatcc
     3121 atcatgcacg taaagagcgc ggcggtcgcc atgccgctcg tgaagtgctc cgcagcacag
     3181 gcgacgtaca gcaacggata gttcacgact gctcccgcgg gttggcgcgc caaggcgatg
     3241 tacgcgaaca gcgtagcgac ctgcagaatc ccgaacgtga ccagcgcccg ccgccgggtc
     3301 aggaaattca acaacagccc cccgaacaag gccccgagca gccctgcgat gaaaccgacg
     3361 ctgcccaata accaacccac gtccgccaag ctcaaaccca agtcagacaa ccagggccgc
     3421 agcatgccgg tggcaaatgc gtcgccggct ttgtacgtga gcagcaacga gatgatgcgc
     3481 agcccgcctg gacgagccac gaaggccgtc cacgatgggc gtccactgac ggcgcgcacg
     3541 aggggcgttg gctctcgctt gaacaggacg ggcaaggtgg ccaaggccgt caaagcggcc
     3601 atcaccaaga acgtgccacg gacctgcagt cgctcgtaca gcaccagcaa cacgcctccg
     3661 ccgatgatca tgccaacgcg atagcccgcc acttggagcc cgttggcgag accgcgctca
     3721 cggatcgaca gcagatccac ggcgagccca tcagtcgcca cgtcctgcgt cgccgccaaa
     3781 agattgagca cgaataccgc catgaacagc ccctgcaagg gccggcgagc ttcacccgca
     3841 cccggcgaga gatcgagcgt gaaagccagc gcaacgaggg tcacgcaggc cgccaactgc
     3901 agcggcacaa tccaagattt gcgccggccg aagcgcacgg acccgtggtg atccacgagc
     3961 ggcgcccata gaaatttcag cgcccacggc gcggccagca acgaggagag gccgatcgcg
     4021 ccgagcgaga gcccttgctt gcgcaagaga accggcaacg cttgggtgaa gaacccaaac
     4081 ggtaggcctt gagagaagta gaggcagccg agcaagccga gcttgcccag agcctgcaag
     4141 cgcccaaccg agccggccac gcccgcatct gcacccgccg gagtggatct cacgaccgta
     4201 ccttccgggt acgggccttg cgggcaacgc tagccgcgcg cgcgtttcga ctgaccggtg
     4261 tacccacgcg aattggaaac gttacgctca gcaagccctg cgccatttgt cttgcgcacg
     4321 ccgcggcgct gcctcgcgga atggtctcac gtgcggtcgc ggcgagcacg aaatagccct
     4381 gaatcaacgc gaacagcgcg gcggccgcag ccatggccga ttcacatcgg aagacggaga
     4441 cggcccggcc acgctcgata ataacctgca aacgcttcag aatttcggcc aaacttcgct
     4501 cgaaggcgga ccgcacctcc tcatcgcgca gggcttcccc gctcagcagc acccaacacc
     4561 gcagcgcctc ggggtcggct ttggcgccgg tacccaggtg catgtccagg aattcagcga
     4621 gttcccgcgt cgggtctgcg ccgtgcgtgg cgagcgcagc atccagccgc tcgacgtggc
     4681 gagccacgag ctgttccaat gcggccagca aaatcgctcg cttgttcgag aagtgatagt
     4741 ggaccaagcc cggcgtgagc gcggcctctc gtgcaatgtc gccgatcgaa gcaccgtcat
     4801 agccgcgctc agccatgacc tttacgaacg ctcgcgtgat ttccgctcga cgctgttcgg
     4861 tgttggtggg tcggggcatg ctcctcgcat tctcacgctg gcggacttgg gcgcgactcg
     4921 actcgcgctc gtctccagcg acggtttata agttggataa ccaaccaatt aacacgccgc
     4981 tccccttcgg tcaaaggcgc gccgagccca ttccgttact agggcgagca aatcccagcg
     5041 ctcgggctca cccggacggg caaaacgctt gcgccgagga ttgcactgcg ccatcagcag
     5101 gcccgtgaag ccgccccccc atcgaacggt cgagccccgg ttcttcgcga ccgcagccaa
     5161 gggcaccgag ccgctgttgc gcgatgaact ccgcgagctc ggcttgcccc tcgtgcgcgc
     5221 cgaccgcgga ggcgtccagt tcggggcgga gccccggcac gctttcgtgg cgtgcctctg
     5281 gtcgcgcatc gcactgcgga ttctttcacc catcgccgag ttcccgtgcc ccgacgaaga
     5341 cgcactatac gacggggtaa gagaggtgga ttggtcgaga tggatcgacg cacgacggac
     5401 cttgctcgtg cgcgccgcgt gccgctcgag ccggctgacg cacacccagt acatctctca
     5461 acgtagcaag gacgcgatcg tcgatcagct gcgcgatcgc accggttcac gccccgacgt
     5521 cgcccgcgaa tctccggatt tgttgctgtt cgtgcacctg gtgaaagacc gggccaccgt
     5581 ctatttggac tacgggggcg actctttgca cgagcacggc ttccgtcatc agagcggggt
     5641 cgcgccgctg cgcgagacct tggccgcagc cctggtgcgc ttctcgggtt gggatcggca
     5701 gtctccattg accgatccca tgtgcggatc gggcacgctg ctgatcgagg ccgggttgtg
     5761 ggcggcgcag cgcgcgcccg gcatttcgcg gcagttcggc tttgaacgct ggctggactt
     5821 cgacgccgag cgacagcgcg agctggcaca gctgcggcag gccgcgcgtg aaacggcgcg
     5881 ccaagacacg cccgtcacgt tgttcggttc ggactcggat gcttccgtgt tggagctcgc
     5941 ccgcgccaac gccgcgcggg tcgggttacg actcgagctg cgcgcggccc cgctgcgcgt
     6001 cgtgaatccc caatccgagc agggcacttt ggtgaccaat ccgccttacg ggcaacggct
     6061 cgccgaaggg ccggaacaac gccgcgaact ggagaacgtg ttgcgccgct tcgcgggtca
     6121 gcagcgagcc ctgatcgtcc cgcaggatta tccgagtacg ctgcgacccg accgctactt
     6181 ggccgtgttc aacggcaacc tggcctgcga attccggcgc tacgaagcgc gccgtgaaac
     6241 ggctcaccga tcatcggaga gctaatccat cggagagagc gcccgagctt ccgggcagtc
     6301 tcgccaacgc aggaccgcgt taccaggaga cggggcagtg cgaacctttc aaccac
//
LOCUS       DASTKU010000207        16724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_40612,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000207 DASTKU010000000
VERSION     DASTKU010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16724
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40612"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFQ61_07830"
     CDS             complement(<1..300)
                     /locus_tag="VFQ61_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954397.1"
                     /translation="MLGLFAGLLLSSAAFAQDSPDGKPRDALLEWDQDLGALYLSLGF
                     RDVIDSQIQDKLSRGLPTTIVLTAALYRAASTEPFSTTAHPRCRRVERDPRSAHEL"
     gene            complement(410..2125)
                     /locus_tag="VFQ61_07835"
     CDS             complement(410..2125)
                     /locus_tag="VFQ61_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9954398.1"
                     /translation="MFGRRLTSGAWACLAVLQVGRAWAQPKLAQDTWGAPQPPISPSD
                     AGNPGVPPEGAPEPAEPRSDPPAAVRVSASGPTTPTGETRTRRRRRELTDGCADCYAR
                     SHLKGAQELNVRYTLEGIEVRGNSRTSPRVILRYVRFKAGDVLDVDDPEVELTRFRLL
                     GTGFFRDVQFSLRKGSDRGLVVLRIDVTERNTIVVNDISMGLSADADTRGHAKRLSAY
                     AGLDVAETNLGGTGITLGAAAAIAEQQRAIRVRFLDPAFLGSPFMTSGYLLFNDTRDY
                     FGNASFKWADPLNNAPTSPAVVRYKRFGGALGVGRDLSVSTQLWLTYRLETINASVPL
                     EASHLRGYAREPIDFHVIPGRSVLSTIGGTLQLDTRDQPVLPTRGWFLSFHGELSLLP
                     LSLDYDYQRFELDVSRWWRLPGHDHVFKLSLFAGGIAGDAPFFEQFYVGDFTDFLPDR
                     VLGVNFDRRPPPNFLGTEIVEVRYGDFAAKIGGEYRIPVYRGRRSIYGIDFFASAGLY
                     AVAGRRDLTHPPLGYSGLARIPVDVTGNLGLRLDTSAGGFTFAFSNVLSFIPVRGEGP
                     AGGRE"
     gene            complement(2125..3660)
                     /gene="malQ"
                     /locus_tag="VFQ61_07840"
     CDS             complement(2125..3660)
                     /gene="malQ"
                     /locus_tag="VFQ61_07840"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET9954399.1"
                     /translation="MHTSPFDRRSSGILLHPSSLPGPHGIGDLGPSAHAFAEFLARAG
                     QRWWQMLPVGPLGGGESPYDSPSSFAGNPLLISLDMLAQDGLLQPSDVVAPARLVNAK
                     TCLFQTARRFKLKRLRHAFERYSERRHEFAEDMASFRARNASWLEGYAAFAALKRAHG
                     GRHWQHFGPEFSHYSPGLVERMSPELRREVECEEFVQFVFDRQWRKLRAHCHHLGIQL
                     LGDVPMFVSHDGADVWQTREAFQLDHAGNRRVVAGVPPDYFSADGQLWGNPLYDWDAL
                     RRTDFGWWVSRMATTLSRFDAVRLDHFIGFVRYWEIPAGAHSAREGHFVPAPGVALFN
                     RFREAFGSLPFLAEDLGIVTDEVHALRDQFGLPGMKVLEFAFGGGRDYQPHRFDPRTV
                     VYTGTHDNDTLMGWLTAYERCEEPKERERLLAERNRALAYVGATDQEPNFAFIRAAMA
                     SVANTAIFPLQDLLGQGTWARMNVPGTSSGNWCYRCSREDLSIPVAQRLAALTETYER
                     LVR"
     gene            complement(3683..4621)
                     /locus_tag="VFQ61_07845"
     CDS             complement(3683..4621)
                     /locus_tag="VFQ61_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET9954400.1"
                     /translation="MQSVASATDWAVFAGVVVCALAVDLALFGRAKQYISFREALIRS
                     TFFLLVGGLFSVYLGIRHGQPTAVTYLVAYLVEESLSVDNLFVFLVLFTYFKISEPRQ
                     QRILFWGIVGAVVMRGIFIVAGTALLSRFRFMMYVFGAFLVFTGAKLLFRKEDSVDPE
                     ESPALKIARRFLRTTPEFDGDRFFLSRDGVRYATPMFLVLIVVEFTDLLFAVDSVPAV
                     LAVSSDVYVVYTSNIMAILGLRALYFVLSGMMGRFHYLGIALSVILMFVGGKMLVHHW
                     YSVPELASLGVIGGVLGAAIIASLLRPERQPSTRDG"
     gene            4765..5607
                     /locus_tag="VFQ61_07850"
     CDS             4765..5607
                     /locus_tag="VFQ61_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954401.1"
                     /translation="MHTCVASAKAAQHPTHRERSGPPNSAQSFESSASYVRLAGYRPR
                     ASVSLLARSAEIASRSLAMRTVIPRAAILLPVLLAALSSCKKDREPGPSGGSALVSPS
                     GAVAPVLAPNAPAANLPAAGTLPSALPLGVTWADPTGWQRLPLQNPMRKATYRVPRAT
                     GDKEDGELAVFYFGPSEGGGLEANVQRWIKQFQDVPAEKVRRAERTQNGLRQHTLEID
                     SGTFSSGMPGGPTQPKAGYGLLGGIVEAPSGAYFFKLTGPSATVQAARPGFTALLDSV
                     KVSQ"
     gene            5630..6541
                     /locus_tag="VFQ61_07855"
     CDS             5630..6541
                     /locus_tag="VFQ61_07855"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET9954402.1"
                     /translation="MPELPDVEVYCERLTALFGGQPLQAARLKSPFLVRTVEPPLSAA
                     VGRTLKSVTRLGKRLVFEFDPELYLVFHLMIAGRFHLKPLGASPGKLGLLALDFPSKT
                     VMLTEASPKKRASLRVFSDLHGVEQQNAGGIEPLASDLAQFRAALLEENHTLKRSLTD
                     PHLFSGIGNAYSDEILFVAQLSPLKRSFDMQPDESERLYRATRKVLTDFTSRIRKEVG
                     EGFPEKVTAFREDMAVHGRFRKPCPVCESPIQRIVYAENEANYCAKCQTGGKLLSDRA
                     LSRLLKGDFPKTLEELEERRQANTALR"
     gene            complement(6615..7067)
                     /locus_tag="VFQ61_07860"
     CDS             complement(6615..7067)
                     /locus_tag="VFQ61_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9954403.1"
                     /translation="MGIQRILVPVDYSDSSLAALSFARDLADGFGAKLDIVHVWDRPT
                     YVSDAVLIGHGDSQRPIGELIRENAEKDMLEFLAKANLPSALANQHRLVSGDPASALL
                     KEIEREKYDLVVVGTHGRTGFAHMLLGSVAEKLVRHSPVPILTVPKRS"
     gene            7206..7739
                     /locus_tag="VFQ61_07865"
     CDS             7206..7739
                     /locus_tag="VFQ61_07865"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9954404.1"
                     /translation="MANEKPSLADDEPNRPETGGPRVAPREQHASGTQRSQSLHHTPS
                     EPEAADLLPRDVNAFDGEVTVVREHSLVTACGRGAVSARLELMRGPGAPRVIPLAAVE
                     TVIGRSNQASVCLESHLLSRRHIALVPQGPGYRLQDLESANGVYLNGVKVHSAVLHEG
                     DVIQIGDVLLVFHEGSA"
     gene            7740..8615
                     /locus_tag="VFQ61_07870"
     CDS             7740..8615
                     /locus_tag="VFQ61_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9954405.1"
                     /translation="MSAYVKFWGTRGSIPTPGHSTKRYGGNTSCVEIRMAGLLFICDG
                     GTGLRELGLALSERGPVEAHMFFSHMHWDHIQGFPFFTPAYTKQNRIHVHEVTPFDNR
                     VHRLLHGQMRSEYFPVEFTDLRASISGRHFENNELHVGDVRVRCLEQVHPGRSFAYSF
                     ECDGKKVVYATDSELDLSIRNREETDRDPSLLRQLPPELVDFIGGADLLIGDAQYTDL
                     EYPARVGWGHPRANTVVDLAVQAGVKQCALFHHDPMQHDQAVDEKVQTGRDRAVAHAS
                     QLGVFGAREGLELKL"
     gene            8757..9257
                     /locus_tag="VFQ61_07875"
     CDS             8757..9257
                     /locus_tag="VFQ61_07875"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET9954406.1"
                     /translation="MKCVLPSHCVSRHPIRVRFGETDLMGIVHHGTYISYFEVGRVEY
                     MRRRGLNYTSWTSMGFHLPVVEAHVRYRRSVRFDELVFIETRLTELMRVKLRFDYRLL
                     RPRGPDQDEELVAEGYTSLACVDQDHVPRRLPAEAEAVLTGPEVEELPLEETPRDYVR
                     AAAAAT"
     gene            complement(9638..9880)
                     /locus_tag="VFQ61_07880"
     CDS             complement(9638..9880)
                     /locus_tag="VFQ61_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954407.1"
                     /translation="MAVAGGEDARTWVGCGACADHGVGAAVAGGEEGRAGPGCAERPT
                     HGIGVAVAGGEDGRAGGGNEPRPAPNGIGVAVAEPP"
     gene            9966..11129
                     /locus_tag="VFQ61_07885"
     CDS             9966..11129
                     /locus_tag="VFQ61_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954408.1"
                     /translation="MPVAPASIALSPVPVPQPQMAPQAQSASSLARTSGSLAPEAARE
                     RVFGADSGTPSSTSSNWSPAHVQSELDGPLSVDEPAFSRRRGVVPFADALWIRVRLAR
                     LELPLWSVLAPGFVLVALISAFAASAVSAHAPTRSKNGWEGQPAPSTVVNAQPPTAPE
                     SAPSSWAVPPTAPASADAPDLRPEFAGPTPVPSFGLAPGSYGSGDVSRLNATRLKQAT
                     DAARSLTAELSRDPGLAQEPKTLEALRRFLDDPETARVVLPAIAELPAPFAADLLYEV
                     WTSSVARTETTELARAFLFSRDVRAKASPALSVALDLKLAESCDQYRAVLTRAVREGD
                     RRSLPLLIRLQRRFGCGPNKRLDCYDCLRKGDDLEQAIKSVRERREVRSFGAR"
     gene            complement(11135..11605)
                     /locus_tag="VFQ61_07890"
     CDS             complement(11135..11605)
                     /locus_tag="VFQ61_07890"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9954409.1"
                     /translation="MQVRTEIEIDAPPERVWSVLLDFARYPEWNPFLVALTGRAGSGE
                     KLQVTVSLPDSHTEREFRARVTRFEDGYELAWIGHLWMKGLLDGEHFFRLQPLPNGRT
                     RFVHGEDFSGLLLKFVLSRLGEAARGFVYMNQALKKRIESSSRGATELKPAPAG"
     gene            11817..12884
                     /locus_tag="VFQ61_07895"
     CDS             11817..12884
                     /locus_tag="VFQ61_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954410.1"
                     /translation="MRLVRAVVASMGPFDRVSVPFADEHGQPRSLTVVHGGSGTGKTS
                     LFQAIATTRPGHAVAQMARHHAPSAVVPSAACEWLLGMDDPARPHPLYVASPNVRWLP
                     EEEAEGLRRREQGYYDRVAQEGGFALLALSGARWFARQPLVLSAPARTVARYDVRAPL
                     ATDDAARAELSRETKQALAYAALSAALHTRGDNEEPRFDLLGQAMHEVVDRVAALAGL
                     RYRGIEPHSFEPVFTEIGASLAGAPTWLFDTLPTRARHLVALAALSVRTLWAAYPERD
                     PRESEGVILVDDVELHQDEHVLRELPGVLVEVLPRAQWILLTSSAVLAASVSVDDVVA
                     LRRAERNASVAVFFGAAALTH"
     gene            13299..14501
                     /locus_tag="VFQ61_07900"
     CDS             13299..14501
                     /locus_tag="VFQ61_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9954411.1"
                     /translation="MPDSSSITEISSQPLQLRRRFSRFWGLVPVCMSGLLSVSCGNHA
                     QGAPREEPDLPRLEGNSIRFSEKFAKRIELKSIEVKKAAVVPSLAVVGTVTFDPEHVA
                     RVGTRLRGLVREVRHFEGDIVKRGEVLASVDSPELGDAQATVLSLQAELDAARRNAER
                     EKKLSEDKLTTLKETEEATARAERFVALLAAAEQKVSALAGVSNKTSSRAIGVHTVSS
                     PLDGTIVERHVSKGQLVEGDHTAFLVANLDHLWVELAVFERSLPSIRVGDEVELRPLG
                     GSAAALKGRVAHVGQVLNAATRSSPVRVEVENKERLLRPGQAVDAVIRAAGASADAGP
                     VVPPSAVTFVDGKPTIFVQDSPTSVRVASVELGASDGQGLHIKKGVEVGQKVVVDGTF
                     ELKSELFR"
     gene            14509..>16724
                     /locus_tag="VFQ61_07905"
     CDS             14509..>16724
                     /locus_tag="VFQ61_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9954412.1"
                     /translation="MLGAIVSWSIRARRAVIVALVVLIGLGIQAARTLPIDALPDVST
                     VQVGVLTTAPGLSALEVERTVTMPIEVALNGTPGAEKLRSVSRSGLSNVTVVFKDGTD
                     VWFARQLVLERLRGIDLPAAAAMPELAPVSTGLGQIFQFVLESDQHSPMQLRTLLDWE
                     IVPRLRSVPGVVEVNTMGGELKQFQVLVDRVRLEARGLHLSDVIEALKSANLNIGGGY
                     VERRKEAFTVRGQGLLRDVQDIGNVVVRADTQGSPVLVQHVADVQIGAALRYGLVTRE
                     GKGEAVNGIVMMLLGANSKTVVQEVAERVREIEPTLPPGVRIQVVYDRADFVGRTLAT
                     VMKNLAEGVAIVTLVLLLFLGSLRAALAVVIGVPAAMSIALFGMHYFKVTGDLMSLGA
                     IDFGFLVDGPIVIVEAVIAATAGRQLVGAARARAYDRIARSVARPVSFAVGIIMLVYV
                     PLLALQGVEGKMFRPMAITMACALLGALVYSIVFFPAVLVTMVPPAKGHGPHWIEWLA
                     RKYAQFVPTALRLRWVLIGGSAAALVFFTWLFSRAGAEFVPRIFEGDAVVAIRRAPSI
                     SIEEARRLDLEAERVLQSFPEVVTTMGRTGRAEVAVDPVGNDSTDILVRLRPPDEWKN
                     ARDLDELSVLFKNAIESKIPGTFVAVSQPIEDLTNEIISGSRADVQIQLFGTDLEEMT
                     RVAKDIGKAVGAVPGTGDLRVERVLGGPTITAQADRARLARHGVRVEDAFQVLQASR"
BASE COUNT         2976 a         5225 c         5522 g         3001 t
ORIGIN      
        1 gagctcgtgg gcggagcggg ggtcacgttc gacgcgacgg cagcgggggt gggcggtggt
       61 ggagaacggt tcggtcgatg cggcccgata aagtgccgcc gtgagcacga tcgtcgtcgg
      121 caacccgcgg ctcaatttgt cttgaatctg ggagtcgatc acgtcgcgaa aaccgagcga
      181 taggtaaaga gcaccaaggt cttggtccca ctcgagcagc gcgtcgcgcg gcttgccgtc
      241 tggcgagtct tgcgcgaagg cggccgaaga gagcaagagc cctgcaaaca gcccgagcaa
      301 tgcccgagag cgcttccacc aggacgcgcc gagccaaatt cggggccgag ctggcgcgct
      361 cgcgccgcgt gtcccgcgag gacgcgagcg ggacgtggtg atggcgcccc tattcccgac
      421 cacctgccgg accctcgccc cgcacgggga tgaagctcag tacgttggag aaagcgaagg
      481 tgaagccgcc ggcgctcgta tcgagcctca ggccgaggtt gccggtcacg tccactggaa
      541 tgcgcgctag ccccgagtag cctagcggcg gatgcgtcaa gtctcgccgg ccggcgaccg
      601 cgtaaagtcc cgcggaagcg aagaagtcga tcccatagat cgagcgtcga ccgcgataca
      661 ccgggatgcg gtactcgccg ccgatcttgg ccgcgaagtc tccgtagcgg acctcgacga
      721 tctcggtacc caggaagttg ggcgggggtc ggcgatcgaa gttcactccg agcacgcggt
      781 cgggcaaaaa atcggtgaag tctccgacgt agaattgctc gaagaatggc gcgtctcccg
      841 cgatccctcc cgcgaacaaa ctcaacttga acacgtgatc gtgaccgggc agtcgccacc
      901 accgtgagac atcgagctcg aagcgttgat agtcgtagtc gagggagagc ggcagcaggc
      961 tgagctcgcc gtggaaggac aagaaccaac cgcgcgtcgg cagcacgggc tggtcacgcg
     1021 tatcgagctg caacgtgcct ccgatggtcg aaagcacgct gcgccccggg atcacgtgaa
     1081 agtcgatcgg ctcgcgcgcg tagccgcgga gatgggacgc ctccagcggg acgctcgcgt
     1141 tgatcgtctc gagccggtag gtgagccaga gctgcgtaga aaccgacaga tcgcggccca
     1201 cgccgagcgc gccgccgaag cgcttgtacc gaaccaccgc cgggctcgtg ggcgcgttgt
     1261 tcaacgggtc agcccacttg aagctggcgt taccgaagta gtcccgcgtg tcgttgaaca
     1321 gcaggtagcc actggtcatg aatgggctac ccaaaaacgc cggatcgagg aaccgcacac
     1381 ggattgcgcg ctgctgttca gcgatcgcgg cggccgcgcc caaggtgatg ccagtgccgc
     1441 ccagattggt ctccgcgacg tcgagcccgg cgtatgccga gagccgcttg gcgtgaccac
     1501 gagtatccgc gtccgcggag aggcccatcg agatgtcgtt gacgacgatg gtgttgcgct
     1561 cggtgacgtc gatgcgcagc accacgaggc cgcggtcgct gcccttgcgc agggagaact
     1621 gtacgtcccg gaagaacccc gtgccgagca ggcggaagcg ggtgagttct acctcgggat
     1681 catcgacgtc cagcacgtct cccgctttga aacgtacgta gcggaggatg acgcgcggac
     1741 tcgtgcgcga gttgccgcgc acctcgatgc cttcgagcgt atagcggacg ttgagctcct
     1801 gcgcgccctt caggtgagag cgcgcgtagc aatccgcaca gccgtcggtc agctcgcgcc
     1861 gccgccgtcg agtgcgagtt tcacccgtcg gggttgttgg tcccgacgcg gagacgcgca
     1921 ctgccgctgg tggatcgctg cgcggctcgg ctggctctgg tgcgccttcc gggggaaccc
     1981 cggggttgcc ggcgtcggag ggcgaaattg ggggctgcgg cgcgccccaa gtgtcttgcg
     2041 ccagcttcgg ttgagcccaa gctcggccca cctgaagaac cgcgaggcac gcccaagccc
     2101 ccgaggtcag ccggcgcccg aacatcaccg aacgagccgc tcgtaggtct cggtcagcgc
     2161 cgccagacgc tgggcaacag ggatgctcaa gtcttcacgg ctgcagcgat aacaccagtt
     2221 gccgctggag gtgcccggta cgttcatgcg cgcccacgtt ccctgtccga gcagatcctg
     2281 gagcgggaaa atcgcggtgt tcgccacgga tgccatggcc gcccgaatga acgcgaagtt
     2341 cggctcttga tccgtcgcgc cgacgtaggc gagggcgcga ttccgttcgg cgaggaggcg
     2401 ttcgcgctcc ttcggctcct cgcagcgctc gtaggcggtg agccagccca tcagcgtatc
     2461 gttgtcgtga gtaccggtgt aaaccaccgt acggggatcg aagcgatggg gttgatagtc
     2521 ccgtccgcca ccgaacgcga actcgagcac cttcatgcca gggagcccga actgatcgcg
     2581 cagcgcatgc acctcgtccg tgacgattcc gagatcctca gccaaaaaag gcaagctccc
     2641 gaatgcctcg cggaagcgat tgaacagcgc tacgccgggg gccggcacga agtggccttc
     2701 gcgggcggaa tgggcgcccg ccgggatctc ccagtagcgg acgaagccaa tgaagtggtc
     2761 cagccgcacg gcgtcgaagc ggctcagcgt ggtcgccatg cgtgacaccc accagccgaa
     2821 gtccgtccgg cgcagggcgt cccaatcata gagcggattg ccccagagct gcccatctgc
     2881 gctgaagtag tcgggaggta cgcccgcgac gacccgccga tttcctgcgt gatcgagctg
     2941 gaacgcttcg cgcgtctgcc acacgtctgc gccatcgtgg gagacgaaca tgggcacgtc
     3001 cccgagcagc tgaatgccca ggtggtggca gtgtgcccga agcttgcgcc attgccggtc
     3061 gaagacgaat tgaacgaact cttcgcactc gacctcacgc cggagctccg gcgacatgcg
     3121 ttcgacgagc ccgggcgagt agtgggaaaa ctccggtcca aagtgctgcc agtgacgccc
     3181 gccgtgagcc cgcttcagcg cggcgaatgc ggcatagccc tcgagccaag aagcattgcg
     3241 cgcgcggaac gacgccatgt cttccgcgaa ctcgtgacgc cgctccgagt aacgctcgaa
     3301 agcgtggcgc agtcgcttga gcttgaagcg acgtgccgtc tgaaataggc aagtcttggc
     3361 gttgacgagc cgcgctggcg ccaccacgtc acttggctgg agcaggccat cttgggccag
     3421 catgtccagg ctgatcagca gtggattccc ggcgaacgaa gaggggctat cgtacggtga
     3481 ttcaccgccg ccgagcggcc ctaccggcaa catctgccac cagcgttgcc cggctcgggc
     3541 cagaaattcg gcgaaggcgt gcgcgctggg gcccagatcc ccaatgccgt gcggccccgg
     3601 caagctgctg gggtgcagca aaatcccgct cgaacgtcga tcgaatggag aggtgtgcat
     3661 gcgatgttcc acagcgagtg ctttatccgt cgcgcgtgct gggttgccgc tcaggtcgga
     3721 gcagtgaggc gatgatggcc gcgccgagca cgccaccgat gacccccagt gaagcgagct
     3781 ccggcaccga ataccagtga tgcaccagca tcttgcctcc gacgaacatg aggatcaccg
     3841 acagggcaat ccccaagtag tggaagcgcc ccatcatgcc ggacaagacg aaatagagcg
     3901 cgcgcaggcc caggatggcc atgatgttgg acgtgtacac tacgtacacg tcgctggaaa
     3961 cagctagcac cgcaggcacg gaatccaccg cgaaaagcag gtcggtgaac tccaccacga
     4021 tcagtactag gaacatcggt gttgcatacc gaacaccgtc gcgactcaaa aagaagcgat
     4081 ccccatcgaa ctcgggcgtg gtccgtagga agcggcgagc gatcttgagt gcagggctct
     4141 cttcggggtc gacgctgtcc tccttgcgga acaacagctt ggcgccggta aagaccagga
     4201 aggcgccgaa cacgtacatc atgaagcgga agcgactgag cagtgccgtg cccgccacga
     4261 tgaagatacc gcgcatcacc accgcgccga cgatgcccca gaacaggatc cgctgctggc
     4321 gcggctcact gatcttgaag taagtgaaca gcaccaggaa aacgaacagg ttgtcgaccg
     4381 agagcgactc ctcgaccagg tacgccacga gatacgtgac ggcggttggc tgaccgtgcc
     4441 gaatcccgag gtagacgctg aacagtccgc ctaccagcag gaagaacgtg ctgcggatca
     4501 gcgcttcgcg gaacgagatg tattgcttgg cacgaccgaa gagcgccagg tcaaccgcga
     4561 gggcgcacac tacgaccccc gcgaagacgg cccaatcggt cgcggacgct acgctctgca
     4621 tcgaagcccg tctgctatca tgctcgggct tcgcgtgcta gacgagcgcg ggtcgccacc
     4681 gaatggggca ccctgaaccg ggtcgccgtt gaaccggttg ccttcaatcg ggtcgcgaac
     4741 aacagccatc gaacaggagg agccatgcac acctgcgtcg ccagcgccaa agctgcccag
     4801 cacccgactc accgagagcg gtcgggcccg cctaactctg cccaatcctt cgagtctagt
     4861 gcgagctacg tgcgcctggc gggctaccgc ccacgcgcga gcgtgagcct tttggcccgg
     4921 tctgcagaaa ttgcctcgcg ctctctcgca atgcgcaccg tcattccgcg cgcggccatt
     4981 ctgcttccgg tgctgcttgc cgcgctcagc agctgcaaga aggatcgtga gccggggccg
     5041 agcggcggtt cggcgttggt gagcccatcg ggagcggtgg ctccagtgtt ggccccaaat
     5101 gcgccggccg cgaaccttcc cgcggcgggg acacttcctt ccgcgctgcc cctaggggtg
     5161 acttgggcgg atccgaccgg atggcagagg cttccgctgc agaatccaat gcgcaaagcc
     5221 acctatcggg taccccgcgc aaccggagac aaagaggacg gcgagctcgc cgtgttctat
     5281 tttggacctt cggaaggtgg gggcttggag gccaacgtgc agcgttggat caagcagttc
     5341 caggacgttc ccgccgagaa agttaggcgc gccgagcgaa ctcagaatgg tttgcgccaa
     5401 cacactctcg aaatcgactc gggcacgttc agctcaggca tgccaggcgg ccccacgcag
     5461 ccgaaggccg gatatggcct gctcggcgga atcgtggaag cgccgagcgg agcatacttc
     5521 ttcaagctca cgggcccgag cgcgacggtt caggcggccc ggcccgggtt caccgctttg
     5581 ctcgacagcg tgaaagtcag ccaatagagt cgccaggcgg agcccccgcg tgcctgaact
     5641 ccctgacgta gaggtctatt gcgaacggct gacggccttg ttcggcgggc aaccgctgca
     5701 ggcggcgcgg ctaaaaagcc catttctggt gcgcaccgta gagccgccgc tttccgcggc
     5761 ggtgggccgc actctgaaga gcgtgacccg cctcggcaag cgcttggtgt tcgagttcga
     5821 ccccgagctc tacttggtgt tccacttgat gattgcgggg cgcttccact tgaaaccttt
     5881 gggggcctcg ccagggaagc tcgggttgct ggcgctcgac ttcccatcca agaccgtgat
     5941 gttgaccgag gcgagcccga agaagcgcgc ttcgttgcgg gtgttctcgg accttcacgg
     6001 ggtagagcag cagaacgcag gtggcatcga gcccctagct tcggatctcg cgcagttccg
     6061 cgcggcatta ctcgaagaga accataccct caaacgaagc ttgaccgacc cgcacctgtt
     6121 cagcggcatt ggcaacgcct actcggacga gatcttgttc gttgcgcagc tctcgccgct
     6181 caagcgcagc ttcgatatgc agccggacga gagcgagcgg ctctaccgcg ctacgcgcaa
     6241 agtgctcacc gacttcacct cgcgcattcg taaagaagtg ggtgaagggt ttccagaaaa
     6301 ggtcacggcg tttcgtgagg acatggccgt ccacggtaga tttcgaaagc cgtgcccggt
     6361 ttgtgaatca ccgattcagc gcattgttta tgccgagaac gaggcgaact actgcgcgaa
     6421 gtgccaaacc ggcggcaagc tactgtccga tcgcgcgctc tcccggctcc tcaagggcga
     6481 ctttcccaag acgctcgagg agctcgaaga gcggcgccaa gcgaacaccg ctcttcgttg
     6541 atctaggcaa tcgaccgcgc tttacgggtg catccggtca aggcatccct gggtcaacca
     6601 tgggcggtgc tcggttagct acgcttggga accgtaagga tcgggaccgg ggagtggcgc
     6661 accaatttct ctgcgacgct gccgaggagc atgtgagcga aaccggtgcg gccgtgagtt
     6721 ccgaccacca ccaagtcgta cttctcacgt tctatttcct tcagcagcgc gctcgcagga
     6781 tctccggaga cgaggcgatg ctggttcgcc aaagccgagg gtaggtttgc tttcgcgagg
     6841 aactcgagca tgtccttctc ggcgttctcg cgaatgagtt caccaatcgg gcgctggcta
     6901 tcgccgtgac cgatcaggac ggcgtcggaa acgtaagtcg ggcgatccca aacgtgaacg
     6961 atgtcgagct tggcgccgaa gccatccgct agatcacgcg cgaatgagag ggcggcgagt
     7021 gagctgtccg agtagtcgac cggtacgaga atgcgctgta tccccatggt ggccgtaggt
     7081 taatgccgtc catgattact gcaatcaccg gattcattgg cttttaggcc gaatcgcggc
     7141 ggtctcgaga cacctgctcg gaggtgtgcc cttttcctcc gtaagccgct agactgcacg
     7201 cttcggtggc gaacgaaaag ccaagcctgg ccgatgacga gccgaaccgc ccggagacgg
     7261 gggggccgcg agtagctccg cgcgagcagc atgcttccgg aacgcaacgc agccagagtc
     7321 tacatcacac gccctcggag ccagaagctg cggatctact gccaagggac gtgaacgcgt
     7381 tcgatggcga agtgaccgtc gttcgtgaac acagcttggt caccgcttgc gggcgagggg
     7441 ccgtttccgc gcgcttggag ttgatgcgcg gtccgggtgc accgcgcgtg atcccactcg
     7501 cggcggtgga aaccgtaatc ggcaggtcca atcaagccag cgtgtgtctg gaaagccatt
     7561 tgttatcgcg ccgtcacatc gcgctcgtac cgcaggggcc gggctatcgt ttgcaagatc
     7621 tcgagagtgc gaacggcgtc tatttaaacg gcgtgaaggt gcactcggcc gtgttgcacg
     7681 aaggggacgt gattcaaatc ggggatgtgt tgctggtgtt ccacgaaggg agcgcgtgag
     7741 tgtccgctta tgtgaagttt tggggcacgc gcggatcgat tccgacgccg ggacattcga
     7801 cgaagcggta cggtggaaac acgtcctgcg tcgagattcg gatggcgggc ttgctgttca
     7861 tctgcgacgg cggcactggg ctgcgcgaac tagggctggc gctgagtgaa cgggggcccg
     7921 tcgaggcgca catgttcttc agccacatgc actgggatca catccagggc ttccccttct
     7981 tcacgccagc gtacaccaag caaaaccgaa ttcatgtcca cgaagtgacg ccgttcgata
     8041 atcgagtgca tcgattgctg cacgggcaga tgcgttcgga gtacttcccg gtggagttca
     8101 ccgatctgcg ggccagcatc agcgggcggc atttcgagaa caacgagctg cacgtggggg
     8161 acgtgcgcgt gcgctgcctc gaacaggtgc acccgggccg tagcttcgcc tactctttcg
     8221 agtgcgatgg caagaaggtg gtgtacgcga cggatagcga gctcgaccta tccattcgca
     8281 atcgtgagga gacggatcga gatccgagct tgctgcgtca actgccgcct gagctcgtcg
     8341 atttcatcgg cggcgccgac ctactgatcg gcgacgcgca atacaccgat ctcgagtacc
     8401 cggcccgcgt gggttggggg cacccgcgtg ccaatacggt ggtcgattta gcggttcagg
     8461 ccggcgtaaa gcagtgcgcg ttgtttcatc acgaccccat gcagcacgat caggcggtgg
     8521 acgagaaagt gcaaaccggt cgagatagag ccgtcgccca cgcgagccaa ctcggcgtat
     8581 tcggcgctcg ggagggtttg gagctcaagc tctaggattg ctgggtacgg gcccttcggc
     8641 cgagctgcgc tgccggtctg cagcgggggc tcgagccggg gccggccgga tttcgtgccg
     8701 cgactgtcac agcaggcttg cgacttgcgg gcccgagcgt ctattttccc gccgccgtga
     8761 agtgtgtgct gcccagtcat tgcgtctcgc gtcacccgat acgagtccgg tttggcgaga
     8821 ccgacttgat gggcattgtc catcacggca cgtacatcag ctatttcgaa gtcgggcgcg
     8881 tggagtacat gcggcggcgt ggcctgaact acacgtcgtg gacgagcatg ggcttccatc
     8941 tgcccgtagt agaggcgcac gtccgctatc ggcgcagtgt gcgcttcgac gagctggtct
     9001 tcatcgagac gcgcttgacc gagttgatgc gggttaagct acgcttcgac taccgcctcc
     9061 tcaggccgcg tggtccggat caggacgagg aactcgtagc ggagggctat acctcgctgg
     9121 cgtgcgtgga ccaagaccac gtgccgcgcc gcttgccggc ggaagccgag gcggtcctca
     9181 cggggcccga agtggaagag ctgcctctgg aagagacacc tcgagattac gtgcgcgcgg
     9241 cagctgcggc gacgtgagca tcgtgcttcc tgcgtggtac cgaaggccca gcggcgcgtg
     9301 gaaacttccc cgcggcgcgc acggcgaggc tagtattcgg cgagatggtt gaccgtaagg
     9361 atgcgccgcc tgcgcggccc tccgtgcccg caccacggcc cccgtcgaca cgcccgccga
     9421 ttggctcgcg tttgccgcca gcgcccccac ctccggcgca agatcgagcg gagccggccg
     9481 aacctcccgc gccgccccgt cggagctccc tcccacctcc actctcgcgg gccccggcgc
     9541 gcagtgcggc tcctgctgct ggtgcgctgg cagagcccac tgctgcggcc tcgcccgagc
     9601 tcgctcaatc cgcgcctcgg cccagcgtgt tacctcctta cgggggctct gcgaccgcga
     9661 cgccgatgcc gttcggtgct gggcgtggct cgtttccacc tccggcgcgg ccgtcttcac
     9721 cacctgcgac ggcgactccg atgccatggg tggggcgctc cgcgcagccc ggtccggcgc
     9781 ggccttcctc gcctccggcg acggcggctc caacgccgtg gtctgcgcac gcgccgcagc
     9841 ccacccacgt ccgagcgtcc tccccaccag caacggccac gccactgcct tgggtcgggg
     9901 gcgcatctcg cgctgccagc gaacggcccg ctccaggacc cgtcgcgccc atcggtgctg
     9961 ccgccgtgcc agtcgcgcca gcgtccatcg cgttgtcgcc ggttcctgtt ccgcagccgc
    10021 agatggctcc gcaggcgcag agcgcttcga gtctggcgcg gacttccgga agcctagctc
    10081 ccgaggccgc gcgagagcgc gttttcgggg ctgactcagg gacgccttcg agcacgtcca
    10141 gcaactggtc ccccgctcac gtgcagagcg agctcgatgg gcctctcagt gtcgatgaac
    10201 cagccttctc gcgacgccgc ggcgtggtgc cttttgcgga cgcgctctgg attcgggtgc
    10261 gcttggcccg cctcgagctt ccgctgtgga gcgtgcttgc cccgggcttc gtgctcgtcg
    10321 ctttaatctc cgctttcgct gcctcggcgg tgagcgctca tgcgccgacg cgcagcaaga
    10381 acggctggga agggcaacct gcaccttcca cggtggtgaa cgcccagcct ccaaccgctc
    10441 cagagtccgc gcctagctcc tgggcggtgc ctccaaccgc gcccgcctcc gcagatgctc
    10501 cggatctacg tccagagttt gcgggcccga ctccggtgcc tagtttcggt ttggctccag
    10561 gctcttacgg ctcgggagac gtgtctcgcc tcaatgcaac gcgcctgaag caagcgacgg
    10621 atgcggcgcg ctcgctgacc gccgagctgt cgcgcgatcc agggctcgcg caagagccga
    10681 agacgctcga ggcattgcgg cgttttctgg acgatccgga gacggcacgc gtcgtgctgc
    10741 ccgcgattgc ggagctcccc gcgcctttcg ctgcggatct gctgtacgag gtttggacga
    10801 gcagcgtggc gcgaacggaa accaccgagc ttgcccgcgc tttcctattc agccgcgacg
    10861 ttcgagccaa ggcctcgccc gcgctcagcg tagcgttgga tctgaagctc gcggaaagct
    10921 gcgatcaata ccgcgcagtg cttacacgcg ccgtgcgtga aggagatcga cgctcgctcc
    10981 cgctcctgat ccgtttgcag cggcgcttcg ggtgcgggcc caacaagcgt ctcgattgtt
    11041 acgattgctt gcgcaaaggg gacgacttgg agcaagcgat caagtccgtt cgcgagcgcc
    11101 gcgaagtacg ctcgttcggc gcgcgctgag gagttcagcc tgcaggcgcg ggcttcaact
    11161 cggtcgcacc acgcgagctg ctttcgatgc gcttcttgag cgcttggttc atgtacacga
    11221 agccccgagc ggcttctccc aagcgagaca gcacgaactt caggagcaac ccggagaaat
    11281 cctcgccgtg gacgaagcgc gtgcggccgt tgggcaatgg ctgcagtcga aagaagtgct
    11341 caccatcgag cagtcctttc atccaaaggt gcccaatcca cgccagctcg tacccgtcct
    11401 cgaaacgagt cacgcgagct cgaaactctc gctccgtgtg gctatctggc aggctcaccg
    11461 tgacctgcaa cttttccccc gagccagcgc gtcccgtgag cgccaccaag aatggattcc
    11521 actcagggta gcgtgcgaaa tccaacaaca cggaccagac gcgttcgggc ggggcgtcga
    11581 tctcaatctc cgtgcgaacc tgcattttgc cgcatcataa ccgactctag tcagccccgc
    11641 tgcggtttcc ggcaggccaa gccgtgcctc tcggctcgtg atgcgggccg agtattcgtg
    11701 tttctcggac ggtaggcaac acgtcgaacg cgggtccccg cggatcccga gttcatgcga
    11761 cctgtggctg acgggagggt ggaaccgcgc gcgcctgtgg tagcgtgatc gcgaacatgc
    11821 gcctggtaag agccgtcgtc gcatccatgg ggccgttcga tcgggttagc gttccgtttg
    11881 ccgatgaaca tgggcagccg cgctcgctta cggtagtgca cggcggctcg ggcaccggta
    11941 aaacgagctt gtttcaggct atcgccacga cgcgccccgg ccacgcggtg gcgcaaatgg
    12001 cccgccacca cgctccgtcg gcagtggttc ccagtgccgc gtgcgagtgg ctgctaggga
    12061 tggatgatcc ggcgcggccg cacccgcttt acgtggcgag cccgaacgta cgttggttgc
    12121 ccgaagagga ggcggagggc ttgcgccgac gcgagcaggg ttactacgat cgcgtggctc
    12181 aggagggggg cttcgcgctg ctcgcgctga gtggcgcgcg ctggttcgct cgtcaaccgt
    12241 tggtgttgag tgctccggct cgcaccgtgg ctcgttacga tgtccgagca ccactggcca
    12301 cggatgatgc tgctcgtgcg gagttgtctc gggaaacgaa gcaggcgttg gcctatgcgg
    12361 ctttgtccgc tgcgctgcac acgcgcgggg acaacgaaga gccgcgcttc gacctgctcg
    12421 gtcaagccat gcacgaggtc gtggatcgcg tcgccgcttt ggccggcttg cgttatcgcg
    12481 gcatcgaacc gcactcgttc gagccggtat tcaccgaaat cggcgcgtcg ctcgcgggcg
    12541 cgccgacctg gttgttcgat acgctcccca cccgcgcgcg tcacctggtc gcgctggcgg
    12601 cgctatcggt aagaacgcta tgggcggcct atccggagcg ggatccgcgt gagtcggagg
    12661 gcgtgattct ggtagatgac gtcgagttgc atcaggacga gcacgtactc cgagagttgc
    12721 ctggcgtgct cgtggaggtc ctgccgcgcg cccagtggat tttgctcacc agctcggccg
    12781 tgctcgcggc cagcgtgagc gtggacgacg tagtggcgct ccgccgagca gaacgtaacg
    12841 cttcggtggc ggtatttttc ggagccgccg cgcttaccca ctgatggtca tggtaggccc
    12901 gcagtttgcg ggtggcctgc ggagctagtt accgaaggct cgtggtggcc cgaagcggga
    12961 cggacttatc gatcgtcgct tcgttcgtgg cttttgctcc gcccttccgc gtgctgcctt
    13021 tgcgggttcg gctgatcttg ccgtcccgtc ggctcgtgcg ttcatgcctt ggtcaatcga
    13081 gagcgctgct tgccgaccgt acttgggggc cccatgtgcc gaggggttgg tcgtcaatga
    13141 attctatttg aagtcgggaa catctttctt ggaggattgt ttcattgggc cgagccgatt
    13201 tgaaaaaatt ttgctatcac gcgcagccgc accgagcgga agcggcagca ttcaaaccgg
    13261 acaatttacc cgcggatttg gagattggag tgaacgtgat gcctgattcg agctcaatca
    13321 cggagatttc gtcgcaacct ctgcagctgc gccggagatt ctcgcggttt tgggggctcg
    13381 ttccggtgtg catgagcggc ctcttgagcg tgagctgtgg aaaccacgcg caaggcgctc
    13441 cgcgtgaaga gcccgacttg ccgcgcctcg aaggtaactc gatccgtttc tccgaaaagt
    13501 tcgcgaaacg catcgaactg aagtcgatcg aagtgaaaaa ggctgcagtc gtcccctcac
    13561 tcgcggtggt cggtacggtc actttcgatc ccgaacacgt cgcgcgtgtg ggtacgcgcc
    13621 ttcgcggatt ggtgcgcgag gtcaggcact tcgagggcga catcgtgaaa cgaggtgagg
    13681 tgctcgcctc cgtagacagc ccggagctcg gtgacgctca agccaccgtg ctctccctgc
    13741 aagccgagct cgatgctgcg cgccgcaatg ccgagcgcga gaagaaactc tccgaagaca
    13801 aactcaccac cttgaaggaa accgaagaag cgactgcgcg cgcggagcgc ttcgtggcgc
    13861 tgctagccgc ggccgaacag aaggtttccg cgctcgccgg cgtgagcaac aaaaccagct
    13921 cacgcgcaat tggcgttcac accgtctcct ctcccctcga cggcacgatc gtcgagcggc
    13981 acgtgtccaa gggccagctc gtcgagggcg accacactgc gttcttggtc gccaacctcg
    14041 accacctatg ggtggaactc gcagttttcg agcgtagttt gcccagcatt cgcgtgggcg
    14101 atgaggtcga gctgcggcca ctcggcggct cggccgcagc cctcaagggt cgagtcgcgc
    14161 acgttggcca ggtattgaac gcagccacgc gcagctcgcc cgtgcgcgtt gaggtcgaga
    14221 acaaagagcg tctactcagg ccaggccaag ccgtggacgc ggtgattcgt gccgcgggtg
    14281 cctccgctga tgcgggcccg gtggtgcccc ccagcgcggt gaccttcgtg gacggaaaac
    14341 cgacgatctt cgtgcaagat tcgcccacat cggtgcgggt cgcgagcgtg gaactcggcg
    14401 cgagcgacgg ccaggggctg cacatcaaga agggcgtgga agtcggtcaa aaggtcgtcg
    14461 tcgacggcac gttcgagctg aagagcgaat tgttccgctg aggatcacat gttgggtgcc
    14521 atcgtttctt ggtcgatccg ggctcgacgt gcagtgatcg tcgcgctcgt ggtgttgatt
    14581 ggtctcggaa ttcaagccgc acgaaccctg cccatcgatg cgttgccgga cgtgtctacg
    14641 gttcaagtcg gcgtgctcac cactgcgccg gggctgtctg ctctggaggt ggaacgtacg
    14701 gtgacgatgc cgatcgaggt ggccttgaac ggcacaccgg gcgcggagaa gctgcgcagc
    14761 gtttcgcgtt ccgggctgtc gaacgtcacc gtggtgttca aagacggcac ggatgtctgg
    14821 tttgcccgcc agctcgtgct cgaacgactg cgcggcatcg atttgccggc cgccgcggcg
    14881 atgccggagc tcgcgcccgt ttccaccggc ctcgggcaga tcttccagtt cgtactcgag
    14941 agtgaccaac attccccgat gcagctacgc acgctgctcg attgggagat cgtgccgcgg
    15001 ctgcgcagcg tgccgggcgt ggtcgaggtc aacacgatgg ggggcgagct gaagcagttc
    15061 caggtgttgg tggacagggt ccgcctcgag gcgcgcgggc tacacttgag cgatgtgatc
    15121 gaggctttga aatcggccaa cctcaacatc ggaggtggct acgtcgagcg tagaaaagag
    15181 gcgttcaccg tcagagggca gggcttgttg cgcgatgtcc aggacatcgg gaacgtagtg
    15241 gtgcgcgcgg atacacaggg ctcaccagtc ttggtgcagc acgtggctga cgttcaaatc
    15301 ggagccgcgc tccgctacgg gctggtcacg cgcgagggca agggtgaggc ggtcaacggc
    15361 atcgtgatga tgttgctcgg cgccaacagc aagacggtgg tgcaagaggt cgccgagcgg
    15421 gtgcgggaaa tagagccgac gttgccgccg ggcgtccgca tccaggtggt ctacgaccgc
    15481 gctgacttcg tcgggcgaac gctcgcgacg gtgatgaaga acttggccga gggcgtcgcg
    15541 atcgtgacgc tcgtgttgct gctcttcctc ggcagcttgc gcgcggcgtt ggccgtcgtc
    15601 atcggcgtgc cggccgcgat gagcatcgcg ctattcggga tgcactactt caaggtaacc
    15661 ggcgacttga tgtcgctagg ggccatcgat ttcgggtttc tggtggacgg cccgatcgtg
    15721 atcgtcgagg ccgtgatcgc ggcgaccgca ggccgtcaac tggtcggggc cgcaagagca
    15781 cgcgcttacg accgcatcgc acggagcgtg gctcggccgg tttcgttcgc agtcggcatc
    15841 atcatgctgg tctacgtgcc gctgctcgcg ttgcaggggg tcgaaggcaa gatgttccgg
    15901 ccgatggcca tcaccatggc ctgcgcgctg ctcggggcat tggtctattc gatcgtcttc
    15961 ttcccagccg tgctcgtcac catggtgcct ccggcaaagg gtcacgggcc gcattggatc
    16021 gaatggctgg cacgaaagta cgcccagttc gtgccgaccg ctttgcgctt gcggtgggtc
    16081 ttgatcggcg gttccgcggc cgctttggtc ttcttcacct ggctattcag ccgcgcgggc
    16141 gcggagttcg tgccacgcat tttcgaaggc gacgccgttg tcgccattcg tcgagccccc
    16201 agcatttcga ttgaagaagc gcggcgcttg gatttagagg ccgaacgggt gctgcagagc
    16261 ttcccagaag tggtcaccac gatgggccgt acgggccgcg cggaagtggc ggtagatccc
    16321 gttggtaacg acagcacgga cattttggta cgcctcagac caccagacga gtggaagaac
    16381 gcgcgcgatt tggacgagct ttcggtgttg ttcaagaacg ccatcgagag caagatcccc
    16441 ggcaccttcg ttgcggtctc gcagcccatc gaagacctca cgaacgaaat catcagcgga
    16501 agccgggccg acgtgcagat tcagctgttc ggcacggatt tggaagagat gacgcgcgtg
    16561 gcgaaggaca tcggcaaggc ggtcggcgct gtcccgggca cgggtgatct gcgtgtcgag
    16621 cgcgtgctcg gcgggcccac gatcacggcg caggccgatc gtgcgcgcct ggctcgccac
    16681 ggcgtgcgtg tggaggatgc atttcaggtg ctgcaagcct cgcg
//
LOCUS       DASTKU010000208         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_486900, whole genome shotgun sequence.
ACCESSION   DASTKU010000208 DASTKU010000000
VERSION     DASTKU010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486900"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..651)
                     /locus_tag="VFQ61_07910"
     CDS             complement(16..651)
                     /locus_tag="VFQ61_07910"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HET9954413.1"
                     /translation="MTLAAPELVTMAPSNAKPEAAVEGPAQAHSSGSAGNGVNDAAAK
                     GPTKRGLFWALFPVGLLGSMVTGLLVMASIASRDPSFALEQDYYAKAVHYDDEVAQRG
                     RNQALGWRANAEEVQPSRVILSLSDGQGTPIAGAVLRVEAIENAHASRRHSAAVTALD
                     GGRYQLEMPNGARGIWELRISANRGTELFTQSIRVEFERSHDSQPGRETRP"
     gene            complement(648..2027)
                     /gene="ccoG"
                     /locus_tag="VFQ61_07915"
     CDS             complement(648..2027)
                     /gene="ccoG"
                     /locus_tag="VFQ61_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HET9954414.1"
                     /translation="MSAPISPERVLPTLNADGTRHKIRPRLYTGPIHSRRKWAAWGLM
                     FAFVALPFARIGGKPAMLLDVAHREFTFFGHTFYATDGVFLMLLLLSIFVSIFWITAL
                     VGRAWCGYACPQTVYMEFLFRPIERWFEGSREAQLRLDKRGLSARRVAKNVVFLGLSA
                     LLGNAFLAYFVGTDALTHWISRSPVEHPSAFLVMAATTGLVFLDFAYFREQLCTVACP
                     YARLQSVLLDKRSLIIGYDSKRGEPRGKGKPKPGDGDCIDCNACVVTCPTGIDIREGL
                     QLECIACGQCADACDSIMNKIKKPQGLIRYGSQLGFATGKQHSLLRPRVVAYPALLSV
                     LLIAMVTLGSQRSDVQLTVLRGLGAPFTQQGGEVISQIRVKIENRSSEAQDFQIDLQK
                     ADGAKLIAPENPLHVKSREQRTTSLFVVSPKANFHKGERSVVVHVKGASLERSVPFRL
                     LGPTDGGLP"
BASE COUNT          401 a          648 c          673 g          373 t
ORIGIN      
        1 ccaggaacgc tagattcaag gtcgcgtctc ccgtccaggc tggctatcgt gcgagcgctc
       61 gaactctacc ctgatggact gggtaaacaa ttccgtgccg cggttggcag aaattcgcaa
      121 ctcccagatc ccgcgcgcac cgtttggcat ctccagttgg tagcgaccgc cgtccagggc
      181 ggtgaccgcg gctgaatggc ggcgcgaagc atgcgcgttt tcgatggctt cgacgcggag
      241 cacggcgccc gcgatcgggg tgccttggcc gtcactcagg ctcagaatca cgcgggacgg
      301 ctgcacctct tccgcgttcg cgcgccagcc cagcgcttga ttgcggccgc gttgagcgac
      361 ctcgtcgtcg tagtggaccg ccttcgcgta gtagtcctgc tcgagtgcga acgaaggatc
      421 acgcgacgcg atgctggcca tcaccagcag cccggtcacc atcgaaccaa gcagccccac
      481 cggaaagagg gcccagaaca ggcctcgttt ggtcgggcct ttggcggccg cgtcattgac
      541 gccgtttcct gcagagcccg atgagtgggc ttgggcaggc ccttcgactg cggcttctgg
      601 cttggcgtta ctcggggcca tcgtaactag ctcgggtgcg gccaaggtca tggcaatcct
      661 ccgtcggtgg gacctagtaa tcggaaggga acgctgcgct cgagcgaagc acctttgacg
      721 tgcaccacca cgctgcgctc acccttgtga aagttggcct tgggcgagac cacgaacaaa
      781 ctcgtggtgc gctgttcgcg cgactttaca tgtagcggat tctcgggcgc gatcagcttg
      841 gcgccatcgg ctttctgcaa gtcaatctgg aagtcctgcg cctcgctgct gcgattctcg
      901 atcttgaccc gaatctgact gatcacttcg ccgccctgct gggtaaaagg agcgccgagt
      961 ccgcgcagca cggtgagctg cacgtcgcta cgctgactgc ccaacgtaac catcgcaatc
     1021 aggagcacgg acagcaacgc agggtacgcc accacccgcg gccgcaacaa agagtgttgt
     1081 ttgccggtcg cgaacccgag ctgagagccg tagcgaatca gaccctgggg cttcttgatc
     1141 ttgttcatga tcgagtcgca agcgtctgca cactggccgc aggcgatgca ctcgagttgc
     1201 aacccttctc ggatgtcgat tccagtcgga caagtcacga cgcaggcgtt gcagtcgatg
     1261 caatcgccat cgcccggctt gggtttgcct ttgccgcgtg gctcaccccg cttcgaatca
     1321 tacccgatga tcaatgagcg cttgtccagc aacacggact gtagccgcgc gtagggacag
     1381 gccaccgtgc acagctgctc acggaagtac gcgaagtcca ggaatacgag gccagtggtg
     1441 gccgccatca ccaagaatgc cgaggggtgc tccaccggcg agcgagagat ccaatgggtc
     1501 agcgcgtccg tgccgacgaa gtaggccaag aaggcgttgc cgagcagcgc cgagagcccg
     1561 aggaagacca cgttcttggc cacgcgccgg gcgctgagcc cacgtttgtc caatctgagt
     1621 tgagcctctc ggctgccttc gaaccatcgc tcgatcgggc ggaacaagaa ctccatgtac
     1681 acggtctgcg ggcaagcgta tccgcaccag gcgcgaccca cgagcgcggt gatccagaag
     1741 atcgagacga agatgctgag cagtaacagc atcaggaata cgccgtcggt cgcgtagaat
     1801 gtgtgtccga agaacgtgaa ctcgcggtgc gccacgtcga gcaacatggc tggctttccc
     1861 ccgatgcgcg cgaacggcaa cgccacgaag gcgaacatca gcccccacgc ggcccacttg
     1921 cgtcgtgagt gaatggggcc ggtgtagagc cgcgggcgga tcttgtggcg cgtgccgtcc
     1981 gcattgaggg tgggcagtac gcgctcgggg gaaataggag ccgacatgac gggagatcga
     2041 acggcgggga ggggggttag ttgacggggg tgccctcggg cgccttgccg ggcac
//
LOCUS       DASTKU010000209        16406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_932082, whole genome shotgun sequence.
ACCESSION   DASTKU010000209 DASTKU010000000
VERSION     DASTKU010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16406
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932082"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..818)
                     /locus_tag="VFQ61_07920"
     CDS             complement(60..818)
                     /locus_tag="VFQ61_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954415.1"
                     /translation="MSLNSNDGLFTVHAGELGATAWSALEALASHRALAIDGVFGVDE
                     CAAYVRAVQDARAEWVSAFGNEQFSLGRAFYTDLEQDRCGEYFRGARASDASVERHLP
                     GMQAALRELVARVTGGRSFPRRGFCGPGVHIFPAGEKVARSGGVVHFDTEGLTPHHLR
                     ARKRALSVIVMLGLPESGAAIRVWDVFYAGRDRATASELRKPSSAFKYSLGGAIVIDS
                     YRLHQILPFQGQQDRISATVHAAEVDPGCWETWF"
     gene            complement(943..1308)
                     /locus_tag="VFQ61_07925"
     CDS             complement(943..1308)
                     /locus_tag="VFQ61_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954416.1"
                     /translation="MASPSLPLIAFAFATTLALQWWGYWVARRQNKRLFRWAARAVLP
                     AALLGIGGVLWGLRGGEEARRVADAAPPADAARAYAQGVSGAMSGAAVLVVLCNVGLL
                     TLIVIFAAGSKARPSGEQE"
     gene            complement(1318..2184)
                     /locus_tag="VFQ61_07930"
     CDS             complement(1318..2184)
                     /locus_tag="VFQ61_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9954417.1"
                     /translation="MLKKTGAGKILESTAVPIGGVRASLYNYDIDGSEPKPEHVAFLN
                     ARVLPVLTGKDARCWLQGSASKTGTDAHNMQLSQKRVEGVRNFLVSRGVSPTRIVSSF
                     VGESLASLKVAEAEGDRAVALLCAPLLSPPPPPPPPAPAGPKRATRFRIRELGGISGG
                     AGIITFDSLYFQIWDFTNNVTTFYNYRAGGFGKGAKGGPPLSATLEGPWNDFSTTGPL
                     ATDEFGGAARFTTAGGAKWSINFVNFMSLPRGLKTNPNPLEISTGFTVGVGVSTSVGE
                     MSLGPTYPFTGP"
     gene            2388..2894
                     /locus_tag="VFQ61_07935"
     CDS             2388..2894
                     /locus_tag="VFQ61_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954418.1"
                     /translation="MNWLSTLWAQYFRREQTISCDAFLSANLVLGCFFSIVLFVALKS
                     LDLSSAITAVLVAAMVPVHLAGLPLSKARPARTRSILLVQGLTLGAAVIAMVIATSVE
                     IAREPVLRNIRYLPGLSVSLLTYAGLEVEFGLNGQASVRSWRRVGLGLGMGGEILLLS
                     CIIGHFVR"
     gene            3014..4348
                     /locus_tag="VFQ61_07940"
     CDS             3014..4348
                     /locus_tag="VFQ61_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954419.1"
                     /translation="MRAPRFWLTPGPALSGAFNGALSVPSQSPILVELLPTGRVRPAD
                     AIDRASEAELPEASRGKAEGRGLALYVQAQTELHYDSPDGLVFGRLHPGALVRVTIEG
                     EGAFLGTAPRVKVGANSRSLGIWGHTAFADRAALSTHVRALSAYQPVTGTEVLRAPLH
                     AWGSPPERPEPMYYVSFSSCYDLFARRSERRLWQYVAGIETFGRDDEGNLDAFDSVIE
                     RSVLPCRARSVSSAAGQLLWHRPELEPQIVSTIPAGYEPIALPDAGSELLLDRVRRGH
                     GELYWIVGRLGKLFCSGWRFSPFRGPREARVEVEVPRDAADAVTTGQLVLQTSMDGKR
                     LSYPVEYVPSTQHQPAQLWLGSPRLGGHPYFKCSCENNYVVLKAFESEVWVSNKELPD
                     DAVAFDPDDKERWFFSRRACEVALGEVQQQLQRDPNSATRLGIHARSNSIPH"
     gene            4631..4837
                     /locus_tag="VFQ61_07945"
     CDS             4631..4837
                     /locus_tag="VFQ61_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954420.1"
                     /translation="MAGGQTVNQKIQAFASSRVGQQVGDGECWTLAEQALAAAGAKTS
                     NEIMGAANVTPDADYVWGKLHQAQ"
     gene            4785..5246
                     /locus_tag="VFQ61_07950"
     CDS             4785..5246
                     /locus_tag="VFQ61_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954421.1"
                     /translation="MSPQTRITFGASFIKLSEVTPGDIIQFRDYSMEKTTVVSVLYPP
                     LPGGAKLDENPVEESVNTESVSEPHHTAIAKSLYSKMRLTVLHQNFGDAGMVVQSTSL
                     ALASIPASKSTTVGTRTLEIGGKQVKLAVKITTTVTVDVSGTIWAYRPIKK"
     gene            5578..5952
                     /locus_tag="VFQ61_07955"
     CDS             5578..5952
                     /locus_tag="VFQ61_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954422.1"
                     /translation="MLGLRIVRHAAGELPAPSAACRLRAGASSSTTTKTLKAVPPEKP
                     GEGEVFVAEPSTQGSCWYEARCEGARTVRARCEQERCECWVLTGTERHEYSFELRGGC
                     RDIRGVLQARCGVTLSQPARER"
     gene            6074..8269
                     /gene="tssI"
                     /locus_tag="VFQ61_07960"
     CDS             6074..8269
                     /gene="tssI"
                     /locus_tag="VFQ61_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HET9954423.1"
                     /translation="MVRIAEVSSVLGEGELLLLSMRGAEALGKPFQYVVELLSESSQL
                     DFGSLLGQPMTISLTLADQSQREFNGLVTDFAIVGGLGRYVRYRAVLRPWLWLMSKRT
                     NCRIFQRRNVPTVVRELFREHGFADFEERLTETARYKELEYLVQYRESDLNFVMRILE
                     HEGIYFYFRHEGGKHILVLADSYSAHEPARGAAEIPYFPPQQGQRRESDHVDGWSCSR
                     HIRSGAFVGRDFDFKKPSAAITPSYKNPQEHAHADYELFDYPADFLDAGTGDQRVRMR
                     LEEVQADHETVEATGNVRALNAGHLFTLSDFPREDQNKEHLVTAVTYELEVNGYESST
                     SVGSELAYRVRLEAIDSKRPFHPARVTPKPVVEGPQTAIVRGEKNQDIWTDEFGRVKV
                     QFHWDRVGKNDENSSCWIRVSQLWAGSGFGGVHLPRIGQEVIVDFLEGDPDRPIITGR
                     VYNQDNKPPYKLPDNATQSGIKSRSSLKGAPSNFNEIRFEDLKGREELYVQAEKDQNT
                     LVKNNQSITVGVNQTISVGANQTISVGANQAISVGATRTLNVTKADTEHFKDTRTTNV
                     DKTDTLTITGKHTGTYDGERHETVKTLDKTEVNGKKETIVHGTYDVTADTHFKVVQGA
                     NSLLIEKKIAGVSEGPVSFTNGPCEIKLEGGKIMLTAAEEISLVCGGASIKLTKSGAV
                     EIAGAQKVSLGSGPSAVAVEPAGANVSGPKIGAHAIGMHEISGALVKIN"
     gene            8285..9547
                     /locus_tag="VFQ61_07965"
     CDS             8285..9547
                     /locus_tag="VFQ61_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954424.1"
                     /translation="MLRLIEKIRQSLEDFIEQRERMLLVVRCRDDDCPILLKLLDELD
                     EASDADLYLLFAIPFVHPQTFVDGLVEEIAKRHELASAALAEENKPPLPPVPPMLRDA
                     RKSPQERLRALILYARSLLPEGARRVVFALAPTQILDHAEYLRLVTAITPQRELEPWM
                     PRVRVVLRDAVVRENEDARRAPHPLVQLSPARSAVLEVDLSTEAIVQDMHASTADDSS
                     PMENRAQALFSLALIDAASARPELAIERLDYLLGYYQSTKNALMQGMVLNALGDTFTR
                     ISEPQRAKDWYEAALLPAAECKNPILLSTLARNLGNTEFALGNYSGARAYFTELDRVS
                     THLVDAESKSAALYWRGMSERRLGQTSQAIQSFELGAKLCRGTDQNKELGLHLDQLRE
                     IYAEQGMSGKALVVEEELNRLASAGGPS"
     gene            9649..10443
                     /locus_tag="VFQ61_07970"
     CDS             9649..10443
                     /locus_tag="VFQ61_07970"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HET9954425.1"
                     /translation="MQAVNLLNEGFAVATQSIAAVFPSFPAATLGSLAMGIPHAHIAH
                     PPSGPAPVPPTPIPPVGTVLLGTCVSVLIGGMPAARCGDLGMNPTCCGIPPIFEVFTG
                     SSNVFIGGGRAARMTDVTFHCKPIPPAGAANRGLAAAMRAMSAAAKVAQVAAIAGDAV
                     EAADAAAEGNAAMAQAMGLSAAMGAAQMAADAIAMAMGAAMGKDPCVPPGTLGAITLG
                     VPNVLIGGFPMPSWMNVAKGMMKLVKGIRRRAGRAGRGRGGVSHRS"
     gene            complement(10636..12615)
                     /locus_tag="VFQ61_07975"
     CDS             complement(10636..12615)
                     /locus_tag="VFQ61_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HET9954426.1"
                     /translation="MKPSWVVWSGFSSALLLGGAFGCSSDSEAGGAALGGASALTGGR
                     TGTAGALAGGRSNEGGVGANSGDNGGKGGASTGGNAAAGTSAGGKTSAITGGAGGSTF
                     GGSMAGTGTSAGANNGEAGSPGGGVPGGGMKNSGGNTSAGTSAVGGAAGNGGMGGTQN
                     PTDCKSDGSVITYPTLPGASKSALYSVTANGAPQFVEKLTKFAPEMQVHYAYFGVAKG
                     CTATVAVTLSENFSSYKLSPKSRNLEATKSGNVVTFSSGPNYLILQADSKELLFILID
                     EEESNPPKVGDANVKSIADYEVDNTGAALVTSKVQSAINAASGATKNILYFPPGRYKV
                     GELSMKSNMTLYLAAGAILDGSTKTSDYAAAGPAVESTSHGVVHFNNVQNAQILGRGV
                     IDGNGAVIRGNSNETPSFKTNVVRIDQSSKILIDGILARDPVFWNTLIYKSDQVTIRN
                     YKVINRRPTTTTYNQTDGVDFDASTNGNLYNAFLYTGDDPMATKTEQEGGIDTKNIVH
                     EKVVAYSNSAACKVGTKTFGKTMDGIVFRDIDIVKSGRGLVIDANDTAVIQNTKYENI
                     RVEAIDSTLIDIEEDRAPDWRTAPNTATVRDLYFTNVSSDVKKPVNIHGKSSSVNVSG
                     VYFKGFTVQGKEVTSQTDSDASWNINQYVSNIKFE"
     gene            13593..14642
                     /locus_tag="VFQ61_07980"
     CDS             13593..14642
                     /locus_tag="VFQ61_07980"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HET9954427.1"
                     /translation="MADTMGVLQEAATSKAEALRVIELVWTDVPPLANELGLLLSALA
                     RATLELFPALYGCEQAAGPTRWSEARVETEAHSITRAVQGANGSACRRILSACHRGQL
                     PSLGKMPRAEQARQFALALDPSRLLVLVAVLNLPESSQALLSLAQGTEWLAGNSRSRV
                     VLVLPAELADRGELDHVSYCSCLFADARSTVKLPQLRAGSQRPRAVEPPPAALASGES
                     TGALLQPLVNVSPVVGTPAKNSEAEQVLSERLSRDQELKRLFAFNQPVVTTSGFRPIV
                     DLLWAAGKLVIEIDGEEHRRLQKFSQDRARDYELLLSGYSVVRFTTWNVVDRTDWVVE
                     RIRQAVRYLNQRERA"
     gene            14639..15358
                     /locus_tag="VFQ61_07985"
     CDS             14639..15358
                     /locus_tag="VFQ61_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954428.1"
                     /translation="MRLNPNPQQTFLILSMLFGKTPAEREPRFSDPSVKALKSVKFRR
                     ELEAAGVVRVERRGRSSHLILEDEAWDFAFKYLHSELPTTPRASKVLEHVLGKLRLFL
                     ESHEHSLLDLLAGETAAEPKPHVEASSAVMSNVRTAGDTAVRVESDATCGPPDYERAV
                     REACLALAHGQTRRRVRLKDLREMVRVERTSLDRTLVAMQTAGRLVLYKLDNPSELSP
                     EDDSAALWIAGQPRHLVYLEA"
     gene            15361..>16406
                     /locus_tag="VFQ61_07990"
     CDS             15361..>16406
                     /locus_tag="VFQ61_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954429.1"
                     /translation="MDALQQALNEADFQWTSHIDSIWRDSPADIPELQRSVREQLRLR
                     LAALARNQESASPLGVPLLGTGGSGKTHVLSAVRREAFESNQYFVLVDMTDVREFWDT
                     MLLGYLRSLDQQREGEPQFGRLLKRLLKISGSGSPTYEQLIAARPPGLINRCNDLIRN
                     LAKLDRSQTREHQDVVRALVLLASDDFDIQERGYQWLQGVGIGEDEGFHHDFVVPQRK
                     PEQIVRGLSWLMNLDAPTVLALDQLDAIVAEHNLASAGGPDSERSPRQLASLSIIQGI
                     AGGLSALRDVTRRTLIVVSSLEATWTILDARAPVTMKDRFEPALLLQPIREATSIRQL
                     VERRLAGAYETRGVT"
BASE COUNT         3094 a         5058 c         5076 g         3178 t
ORIGIN      
        1 gcggaagacc tgcaagctcc caagttcaat tatctgcggc ctctcatcta gcctgtcggt
       61 caaaaccacg tttcccagca acctggatcc acctcggccg cgtgcaccgt cgcggagatg
      121 cgatcctgtt ggccctggaa aggcaggatc tggtgcaatc gatagctgtc gatgacgatc
      181 gcgcccccga gtgaatactt gaatgcgctc gacggcttcc gtagctcgga cgccgtcgcc
      241 cggtcacggc cagcatagaa tacatcccac actcggatcg ctgcgccgct ctcgggcaga
      301 cccaacatca cgattacgct caacgctcgt ttacgagctc gcaagtgatg aggggtgagc
      361 ccttctgtat cgaagtgcac gacaccgcca gaccgcgcca ccttctcacc tgccgggaag
      421 atgtgcaccc cgggcccgca gaaaccccgt cgagggaaac tcctgccgcc ggtcacgcgc
      481 gccaccaact cgcgcaaagc cgcctgcatg cccggcaagt gccgttcgac cgaagcatcc
      541 gaagctctgg ccccgcgaaa gtactcgccg cagcgatctt gctccagatc cgtgtagaat
      601 gcgcgaccca aggagaactg ctcgttgcca aacgcgctga cccactccgc gcgcgcgtcc
      661 tgcacggctc gcacgtacgc ggcacactcg tccactccga agacgccgtc gattgcaagc
      721 gcgcggtgac tcgcgagtgc ctctagggca gaccacgcag tggcgccgag ctcgcctgcg
      781 tgaacagtaa ataggccatc gttgctattg agggacatcc tgttcgagtg agtcgtgctc
      841 cagccgcgag aatgtagctc aagattgccg cggcgtctcc gggcggccgg aatcctgcgc
      901 tcctgaaagg ctcgagccaa tcaccgcagg aattacggat ccttactctt gttctccgct
      961 cggacgcgcc ttagagcctg ccgcgaatat gacgatcaac gtcaggagcc caacgttaca
     1021 gagcacgacc aagaccgcgg cgccgctcat ggctcccgat acaccctgag cataggcgcg
     1081 tgccgcatcg gcgggcgggg ccgcgtccgc gacgcgacgc gcttcctctc ccccccgcag
     1141 gccccagagc acccctccaa taccgagcaa tgcggccggc aagaccgccc gcgccgccca
     1201 ccgaaagagc cgcttgttct ggcgacgagc aacccaatat ccccaccact gcagagcaag
     1261 agtggtcgcg aacgcaaatg cgatcagcgg cagtgacggc gacgccatgg ctgcttttca
     1321 gggtccggtg aaaggataag ttggtccgag cgacatctca cccacgctgg tagagacgcc
     1381 aacgcccacc gtgaagccgg tgctgatctc gagcgggttc gggttggtct tgagccctcg
     1441 tggcagagac atgaagttga cgaagttgat cgaccacttt gctccgcccg cagtcgtgaa
     1501 gcgggccgct cccccgaatt catccgttgc cagagggccg gtcgttgaaa aatcattcca
     1561 gggcccttcg agcgtggccg acagcggggg gccgcctttc gcccccttgc cgaagccacc
     1621 ggcacggtag ttgtagaagg ttgtcacgtt gttggtgaag tcccagattt gaaagtacag
     1681 ggaatcgaag gtgattatcc cagctccgcc cgaaatccca ccgagctcgc ggattctgaa
     1741 gcgcgtggct cgtttggggc ccgccggtgc gggcggcggc ggaggtggag gcgggctcaa
     1801 gagcggcgca caaagcaagg ccaccgcgcg atcgccttcg gcttcagcca ctttgaggct
     1861 cgccagggac tcgcccacga agctcgaaac gatacgggtc ggggagaccc ctcgtgatac
     1921 taggaagttt cgaactcctt ccacccgctt ctgagagagc tgcatattat gcgcgtcggt
     1981 gcccgtcttg cttgcagagc cctgcagcca gcagcgcgca tcttttccgg tcaaaacggg
     2041 gagaactcgg gcgtttagaa acgccacgtg ctccggtttt ggctcactcc cgtcgatgtc
     2101 gtaattgtaa agagacgcac gcaccccacc gattggcact gcagtgctct ccaagatctt
     2161 gccggcaccc gtctttttca gcattggcta cctcacaccg ctagctcgaa ctgctacctc
     2221 acacaccgaa caacccagcc cgaccttacc gctccggcgg gtcctggtca attcgttagg
     2281 gctaacgagc ctgcgtttgg ctggggtata aagctatcgc tcatgcggcg tatggatctc
     2341 gtgctcgggc gtgacctaat ctctccattc gccgattgga ttcagccgtg aactggttat
     2401 cgaccctgtg ggcccagtac tttcgtcgcg agcagaccat ttcatgcgac gcattcctca
     2461 gcgccaactt ggtgttggga tgtttctttt cgatcgtact cttcgtggcc ctgaagtcgc
     2521 tcgacctctc gagcgccatc acggccgtgc tcgtcgccgc gatggttccg gtccacttag
     2581 cagggttgcc cctgagcaaa gctcgacccg cacggactag aagcattctg ctcgttcagg
     2641 gtctcaccct cggtgcggcg gtaatcgcga tggtcatcgc aaccagcgta gagatcgcgc
     2701 gcgagcccgt gctccgcaac atacggtatc tgccagggct ctcggtgtcc ttgctcacgt
     2761 acgccgggtt agaagtggag ttcggactga atggccaggc tagcgtccgg agctggcgtc
     2821 gcgtagggct tggtctcgga atgggcggcg agattctgtt actcagctgc atcattggtc
     2881 acttcgtcag atgaactcag gtgctccagg gcgtgcaaga acgttgcttc tgatcgcaac
     2941 cgcgtgtgcg ctcggggcgt gtgagccgcg ctccattgag cggaaggttc cagagtcgga
     3001 tgcggggatc cccgtgaggg cgccacggtt ttggctcact ccgggtccgg cgttgtcggg
     3061 cgcgttcaac ggtgccctga gtgtcccgag tcaaagtcca atactggtcg aattgctgcc
     3121 aacgggtcgc gttcgtcctg ccgatgcaat tgatagggcg agtgaggcgg agctgccgga
     3181 agcgagtcgt ggcaaagcgg aggggcgtgg gcttgcgctg tatgttcaag cgcaaacgga
     3241 gcttcactac gactcccccg atggactcgt gttcgggcgc ctccaccccg gcgccttggt
     3301 acgtgtgacg atcgaagggg agggggcgtt tctcgggacc gcaccgcgcg taaaagtggg
     3361 tgccaactcg cgttcactcg ggatctgggg gcacacggcg ttcgctgacc gcgccgcgct
     3421 gtctacgcat gtcagggcac tgagcgctta ccagccggtg actggaaccg aggtgttgcg
     3481 ggcgccgctc catgcttggg ggtcgccccc cgagcggccg gaacccatgt attacgttag
     3541 tttctcatcc tgctatgact tgttcgctcg acgtagcgag cggcgcctct ggcagtatgt
     3601 cgcggggata gaaaccttcg ggcgcgatga cgaaggcaat ctcgatgcat tcgattcagt
     3661 catcgagcgt tccgtattgc cttgcagagc tcgctccgtc tcgagcgccg ccggtcagct
     3721 gctttggcac cgccccgagc tggagccgca aatcgtgtcg accatccccg ctgggtacga
     3781 gcctattgct cttcccgacg ccggatccga gctcctcctg gatcgagtgc ggcgaggtca
     3841 cggagagctc tattggatcg tgggcaggct agggaagctc ttctgctcgg gttggcgctt
     3901 cagcccgttt cgcggtccgc gtgaggctcg cgtcgaagta gaagtcccac gtgacgcggc
     3961 agacgccgtg accaccggtc aactcgtctt gcagacctcg atggacggca aacgtttgag
     4021 ctatcccgtt gagtacgtac cctcgacaca gcaccagccc gctcagctgt ggcttggcag
     4081 cccgcgcctg ggcgggcacc cgtatttcaa atgctcctgc gagaacaact acgtagtcct
     4141 gaaggcgttc gaaagtgagg tttgggtcag caacaaggaa ctgccagatg acgcggtggc
     4201 attcgaccca gatgacaagg aacgttggtt cttctcgcgc cgcgcctgcg aggttgcgct
     4261 cggagaagtg cagcaacagc tgcagcgcga cccgaactcc gccacgcgcc tcggcattca
     4321 cgctcgctca aactcgattc cgcactgagc taggcagcgt gaccatgcca cccactcgtt
     4381 tgttactcac aagaacttcc gttgagcata atgacgagcg ctctcgtatg cgcgaaccga
     4441 acatcgccga atcgagaacg agagacttct tcgatacgct ctgcgagggc ttccagctgc
     4501 gggatagaga actcgaagga tctcacagcg gctctctttc gccctaatcg atatcattcc
     4561 tgttgggcgc atcgagttgg aaaggtcgcg tgcggtagcg cttagctgct gcgagtgata
     4621 cacgtgggat atggcaggtg gtcaaacggt aaaccagaag atccaggctt tcgcttcgag
     4681 tcgggtgggg cagcaggttg gggatgggga gtgttggacg ctggcagagc aggcgttggc
     4741 ggccgcgggt gcaaagactt cgaatgaaat catgggcgcc gcgaatgtca ccccagacgc
     4801 ggattacgtt tggggcaagc ttcatcaagc tcagtgaggt cacgcccggc gacatcatcc
     4861 agtttcgcga ctactcgatg gagaagacaa cggtcgtttc ggtgctttac ccaccgttac
     4921 ccggcggggc caagctggac gagaaccccg tcgaagagag cgtgaacacc gagagcgtca
     4981 gcgaacctca ccatacggcc attgccaaga gcctttactc caagatgcgc ttgacggtgc
     5041 tccatcagaa tttcggcgac gcgggtatgg tggttcagtc gacgtcctta gcgctggcca
     5101 gcattcctgc gagtaagagc acgacggtgg gcacgaggac gctggagatc ggtggcaagc
     5161 aggtcaagct ggcggtgaaa attaccacta cggtgacggt cgatgtgtcc ggcacgattt
     5221 gggcataccg cccaatcaag aagtgacaca cgctctaggc acatgacgag gcgaactgcg
     5281 cagtcgagag tcagccgttc acgaccccgc tcacgagcac gcggctcggg gctgggcttg
     5341 cgccaggtcg ccgtcgtgac ggcgctgtgg attggctcga gctgtcaggg accagtgcgg
     5401 cactcggcgg atgcgtcgcg ggtgagtgct ggctccgctc cgagtgcccc ggtgccctct
     5461 gcaagctcgg cgagtgctcc cctgccgatc gcgtcggctc aatcgttggc aagcccgaca
     5521 gccgcagccc cgacgagcag cgcgccaggt ccatcgtcgt cggagcccac cgcgacagtg
     5581 ttaggccttc gtattgtccg tcacgcggcg ggagagctgc ccgcgccgag cgcggcgtgc
     5641 cgactccgag ccggagccag ctcgagcact accaccaaga ctttgaaggc tgtgcctcct
     5701 gaaaaaccag gagagggtga ggtgttcgtg gcggagccgt ccacccaggg ctcctgctgg
     5761 tacgaggctc gatgcgaagg ggcacgcacc gtgcgtgcgc gctgtgaaca ggagcgatgc
     5821 gagtgctggg tgctcacggg cacagagcga cacgagtact ccttcgagct ccgaggcggc
     5881 tgccgtgaca tccgaggcgt gttgcaggcg cgttgcggcg tgacgctgag ccaacccgct
     5941 cgcgagcgct gagtcgcacg agcttcgagg ttgcggcgct cctgctctcc ggtgagggac
     6001 cggaagcacc ggacaggttg acggaaaccc gaaaggcgtg gtgatctggt ccggctggtc
     6061 actgggtgca aagatggtcc gaatcgccga agtttcgagt gtgcttgggg aaggggagtt
     6121 gctgctgctg tccatgcggg gggcagaggc gctcggtaag ccgttccagt acgtggtcga
     6181 gctcctgagt gagagttcgc agctggactt cggctcgttg ttgggccagc ccatgacgat
     6241 ctcgttgacg ttggcggatc agagtcagcg cgagttcaat ggcttggtca ccgacttcgc
     6301 gatcgttggc ggcctggggc gttacgtgcg gtatcgagcg gtattgcggc catggctttg
     6361 gctgatgagc aagcgcacga actgtcggat ctttcagcga cgaaatgtgc ctacggtcgt
     6421 acgggagttg ttccgcgagc atggcttcgc cgatttcgag gagcgactga cggagacggc
     6481 acgctacaag gagctcgagt acctggtgca gtaccgcgaa tcggatctga atttcgtgat
     6541 gcggattctg gagcacgagg gaatctactt ctactttcgt cacgagggcg gcaagcacat
     6601 cttggtcttg gccgactctt acagcgcgca cgagcccgca cgaggcgccg cagagatccc
     6661 ctatttccct cctcaacaag ggcaaaggcg tgagtctgat cacgtagatg gctggtcgtg
     6721 ctcgcgccat atccggtcgg gcgccttcgt gggtcgagat ttcgacttca agaagccgag
     6781 tgccgccatt acgccgtcgt acaagaatcc gcaggaacac gcgcacgcag actacgagtt
     6841 gttcgattat cctgcagatt ttctggacgc agggaccggc gaccagcggg ttcggatgcg
     6901 cctcgaggag gtgcaggcgg atcacgagac cgtggaggcg accggcaatg tgcgcgcgct
     6961 caatgccggg cacttgttca ccctgagcga ctttccgcgt gaggaccaga acaaggagca
     7021 cctcgtcacc gccgtgactt acgagctcga ggtcaacggc tatgaatcga gcacctccgt
     7081 cggcagcgag ctggcttatc gcgtgcggtt ggaggcgatc gatagcaagc ggccctttca
     7141 tcccgcccgc gtcacgccga agccagtcgt cgaggggccc caaaccgcca tcgtgcgcgg
     7201 tgagaagaac caggatattt ggaccgacga gtttgggcgc gtgaaggtgc agttccactg
     7261 ggaccgggtc ggcaagaacg acgaaaatag ctcatgctgg attcgagtct cgcagctttg
     7321 ggcgggcagc ggcttcggtg gcgttcactt gccgcgtatc gggcaagagg tgatcgtcga
     7381 ctttctggaa ggtgacccgg atcgccccat catcaccggt cgtgtgtaca atcaagacaa
     7441 caagcctccc tacaaactgc cggacaacgc gacccaaagc ggcatcaaga gccgcagcag
     7501 cctcaaaggt gctcccagca acttcaacga gattcggttc gaggatttga aaggcagaga
     7561 ggagctctat gtccaagccg agaaggacca gaataccctg gtcaagaaca atcaaagtat
     7621 cacggtcggc gtaaatcaga cgatttccgt gggggcaaac cagaccatat cggtgggagc
     7681 caatcaggcc atcagcgtgg gcgcgacgcg gaccctgaac gtcaccaagg ccgacactga
     7741 gcatttcaaa gacacgcgca ccacgaacgt cgacaagacc gacaccttga ccatcacggg
     7801 caaacacacc ggcacctacg acggtgaacg gcacgagacc gtcaaaacct tggacaagac
     7861 cgaggtcaac ggcaaaaagg aaaccattgt gcacggcacc tacgacgtca ccgcggacac
     7921 gcacttcaag gtcgtccaag gcgccaattc gctcttgatc gagaagaaga ttgcgggcgt
     7981 gtccgagggg ccggtgagct ttaccaatgg gccgtgtgag atcaagctcg agggcggcaa
     8041 aatcatgctc accgccgccg aggagatctc gttggtttgc ggcggcgcga gcatcaaact
     8101 caccaagagt ggggcggtcg agattgccgg cgcgcagaag gtcagcctcg ggtcgggccc
     8161 cagcgcggtg gcagtcgaac ccgcgggagc caacgtcagt ggcccgaaga ttggggccca
     8221 cgccattggc atgcacgaga tcagcggagc tctggtcaag atcaactgaa agaggagccc
     8281 gccgatgctg cgcctcatcg agaagatccg acagtcactc gaagacttca tcgagcaacg
     8341 agagcggatg cttctggtgg tacgttgccg tgacgacgac tgccccatcc tgctgaagct
     8401 gctggatgag ctggatgaag cttcggatgc ggatctctat cttctgtttg cgattccgtt
     8461 cgtgcacccg cagacattcg tagacgggct cgtagaagag atcgcaaagc gtcatgagct
     8521 cgcgagcgcg gcgctggccg aagaaaacaa accccctctg ccgcccgtcc cgccgatgct
     8581 gcgggatgcg cgcaaatcgc cccaagagcg gctccgggcg ctgattctct atgcgcgcag
     8641 tcttctcccg gaaggcgcgc ggcgtgtggt gttcgcgctc gcgcccactc agatcttgga
     8701 tcacgcggaa tacttgaggc tggtcacggc gatcacccct cagcgcgaac tcgagccctg
     8761 gatgccgcgc gtgcgtgtcg tgcttcgaga tgccgtggtt cgggagaacg aggatgcgcg
     8821 gcgggctccg cacccattgg tgcagctatc gccggctcgc tccgcggttc tcgaggtcga
     8881 tctcagcacc gaggcgatcg tgcaagacat gcacgcttcc actgcggatg acagctcgcc
     8941 gatggagaac cgcgcgcagg ccctcttctc gttggctctg atcgacgccg cttccgctcg
     9001 gccggagctc gcgatcgaac gcctcgatta cctgctcggg tattatcaat ccaccaagaa
     9061 tgccctgatg cagggtatgg tcttgaacgc cctcggtgac accttcacgc gcatttccga
     9121 gccgcagcga gcgaaagact ggtacgaggc cgcattgctt cccgctgccg agtgcaagaa
     9181 cccaatccta ctttcgaccc tggcgcgcaa cctcggaaat accgagtttg cgctcggcaa
     9241 ctactccgga gctcgcgcat acttcaccga gctagaccgt gtctccaccc acctggtgga
     9301 cgcggagagc aagtcggccg cgctctattg gcgaggtatg agtgagcgac ggctgggtca
     9361 aacgagccag gcgattcaga gcttcgagct cggagcgaag ctgtgtcgcg gcacggatca
     9421 gaacaaggaa ttgggtcttc acctcgacca gctccgcgag atctacgccg aacagggcat
     9481 gagcggtaaa gcgctcgtag tggaagaaga gctcaatcga ctcgcgagcg cgggggggcc
     9541 gtcatgactc aaggcttgcc gagagcgccg cgaccagtcc cgcccggtaa accgcacaac
     9601 gcgctgcata cgatctcaga gagcatcaac gccgttgtgg gcgcgccgat gcaagccgtg
     9661 aacctcctga acgagggctt tgcggtggcc acgcagtcga ttgcggccgt attcccatcc
     9721 tttcccgcgg caacgctagg atcactcgcg atgggcatcc cgcacgctca catcgcgcat
     9781 ccgccgagtg gtcccgcgcc cgtacctccc actcccatcc ctccggtggg tacggtgtta
     9841 ctcggcacgt gcgtgagcgt gttgatcggc ggcatgcctg cggcgcgttg cggtgatctc
     9901 ggcatgaacc ccacctgctg cgggatcccg cccatattcg aggtcttcac gggctcctcc
     9961 aacgtgttca tcggcggcgg gcgcgccgcg cgcatgaccg atgtgacctt ccactgcaaa
    10021 cccataccgc ctgcgggcgc ggccaatcgt gggctcgccg cggcgatgcg cgcgatgtcg
    10081 gcggcggcca aggtcgccca ggttgcagcc atcgcgggcg acgccgtcga ggccgcggac
    10141 gccgccgcgg agggcaacgc ggcgatggct caagccatgg ggctcagcgc agccatggga
    10201 gcggctcaaa tggccgccga cgccatcgca atggcgatgg gcgcggccat gggcaaggac
    10261 ccctgtgttc ccccgggtac gctcggggcg attactctcg gcgtgcctaa cgtgctgatc
    10321 ggtggctttc caatgcccag ttggatgaac gtcgccaaag gcatgatgaa gctcgtcaaa
    10381 ggcattcgtc gccgcgccgg gcgtgctggc cgggggcggg gcggcgtgag tcacaggagt
    10441 tagcgaaaag ggctcaagcg cacgccactc gcatgcgcca gcacgcggag gtgctgcctc
    10501 gagaactcga ggccgggtcc cgctgatcgt accgatagag cgcggcctcg cgcgtttcca
    10561 cagcggcgcc tgggcccgtc gactcgggct cggcggttca ccaggcgccc ttgacttggc
    10621 ctctattcga gccggttact cgaacttgat attggaaaca tattgattga tgttccagct
    10681 cgcatccgag tccgtttggc tggtcacttc cttgccttgc acggtgaagc ctttgaagta
    10741 gacgccgcta acgttcacgc tggaggattt gccgtgaatg ttgacgggct tcttcacgtc
    10801 cgaggagacg ttcgtgaagt agaggtcccg caccgtggcc gtgttggggg ccgtgcgcca
    10861 gtccggggct cgatcttctt cgatgtcgat cagggtactg tcgatggctt cgacgcggat
    10921 gttctcgtac ttcgtatttt gaatgaccgc cgtgtcgttg gcgtcgatga ctaggcctcg
    10981 gccggacttg acgatgtcga tgtcacggaa gacgatgccg tccatagtct tgccgaaggt
    11041 cttcgtgccg accttgcaag cggccgagtt gctgtaggcg acgacctttt catgcacgat
    11101 attcttcgtg tcgatgccac cctcttgttc ggtcttggtc gccatcgggt catccccggt
    11161 atacaagaag gcgttgtaca ggttgccgtt cgtcgaggcg tcgaagtcca cgccgtccgt
    11221 ttgattgtaa gtggtcgtgg tgggccggcg gttgatgacc ttgtaattcc gaatcgtcac
    11281 ctggtcactc ttgtagatca atgtgttcca gaagacggga tctcgagcca ggatcccgtc
    11341 gataaggatc ttcgagctct gatcgattct caccacgttg gtcttgaacg agggcgtctc
    11401 attgctgttg ccacgaatca cggcgccatt gccgtcgatt actccgcgac ccaagatctg
    11461 ggcgttctgc acgttgttga agtgcaccac cccgtgcgag gtgctctcca ccgcaggccc
    11521 ggccgccgcg taatcactgg tcttggtcga cccatctaga atggcgcctg ccgccagata
    11581 gagcgtcatg ttgctcttca tcgagagttc acccaccttg taccggccgg gggggaaata
    11641 cagtatgttc ttggtagcgc cggaggcggc gttgattgcg gattgaacct tggaggtgac
    11701 gagcgcggcc ccggtgttgt ccacctcgta atccgcgatg ctcttgacgt tcgcatcacc
    11761 caccttgggt ggattgctct cctcttcgtc gatcaggatg aacagcagct ccttggaatc
    11821 ggcctgcagt atcaagtagt tcgggccgct gctgaaagtg accacgttgc cgctcttggt
    11881 tgcctcgaga tttctgctct tggggctcag tttatatgac gaaaagttct cactcagcgt
    11941 gaccgcgacc gtcgcggtgc agcccttcgc gaccccgaaa taggcataat gaacctgcat
    12001 ctccggcgcg aacttggtca gtttctccac gaattgcggc gctccgttag cggtgacgct
    12061 atatagagca ctcttgctcg cgcccggcag cgtcggatag gtgattactg acccgtcgga
    12121 cttacagtcc gtgggattct gggtaccgcc cattcctcca ttcccagctg ctccacccac
    12181 agcgctggtg ccggccgacg tattacctcc cgagttcttc atgccgccgc ccgggactcc
    12241 gccgcctggg cttcctgcct cgccgttgtt ggcgccggca gaagttccgg tccccgccat
    12301 gctgcctccg aacgtgctgc cgcccgcgcc gccggtgatt gcgctcgttt tccccccggc
    12361 tgatgtgccc gcggcggcgt tgccacccgt cgaagcgccg ccctttcccc cgttatctcc
    12421 gctattcgcg ccgacgcctc cctcattgga tctgccgcct gccagggctc cagccgtgcc
    12481 ggtgcgtcca cccgtgagcg cgctcgctcc ccccaacgcg gcacctcccg cctcactgtc
    12541 cgaggagcac ccgaatgctc ctccgagcaa caacgcgcta gaaaaaccgg accacacgac
    12601 ccatgaaggt ttcatctttg ctcctcgacc gcgactcgac tcggcgctgt tccgcgcctt
    12661 gctacctatg cccgcccgcg gtgactttaa tcacgacccc aggcctgatt cggcgagccg
    12721 cggcaactag agatcctttt cgcgtcccgg gaatgttccc tctcccacgt tcaaacatgc
    12781 cttgcggtga ggcagcactt ttgcagccct acctgttcag agtgtcgaat gccgtgttgg
    12841 ccgattcggc atttctcgac atcacgaaac aaccaacacc ggattcatcc gcaggcttct
    12901 atcgaggcca ctccgcttac gagcgcgacc gcccgttgca tcgccccgcc tttccggccc
    12961 attcctcctt tcccgtcaca acgccgcgcc ggcccccatc ttcaacgcag tcccgactgc
    13021 gtcgaagtcc gcgacgcgcg gcaccccagt tgaccctgtg ccgtcgcaag gcaagctgtt
    13081 gcggccgacc tgggaagcgg cggctcgatt gccggctacc acccacgccg acgcttaccc
    13141 tttgcaagac caaagcaacg gagttgcaaa ttacaatcaa tattgtcgca aggacctcag
    13201 gatgagcttt gcgcgcacca ccactccgcg gtcgcagtac gcgagcaact gtcgccactt
    13261 ggcccggtgg ggtcctgtgc tcactttcgc gaaacaggca gcatacgaac tagaatgtcg
    13321 aaaagcgaca tacctccctg gaggtctgtc gaagtaacgg tgagacgcga gacggaccct
    13381 gtaacgcgcg aaggatagaa gatctgaaca tccggtatct gcaggtcgtc atgtgagaaa
    13441 gatccgtacc tggaaaaccc agtgacgaga cgccacactg gatcacagtc agtgttgcgt
    13501 ttgcagggtt ccgaatgagg acggaagtcg atctgggaag gtggcttgag acgctgggga
    13561 ctgggcgagc gggctgcgtc ttctcagtcg atgtggcgga cacaatgggc gtgctgcagg
    13621 aggcggccac ctcaaaggca gaggctctgc gcgtcatcga gttggtctgg acggacgtac
    13681 cgccgctcgc caacgaactg ggactgctgc tgtccgcatt ggctcgggca acgttggaac
    13741 tatttccggc cttgtatggc tgcgaacaag ccgcggggcc gacccgctgg tctgaagctc
    13801 gggtcgagac ggaagcgcac tcgatcactc gagccgtgca gggtgccaat ggctctgctt
    13861 gccgtcggat cttgagtgct tgtcaccgcg gtcagctccc gtccctcggc aaaatgccgc
    13921 gcgccgagca ggctcgtcaa ttcgcgctcg cgctcgaccc gagtcgcctc ttggtcctag
    13981 ttgcggtgct caatcttccg gagtcttcgc aagccctctt gtccctcgcg caagggactg
    14041 agtggctcgc tggaaactca cgcagccgcg ttgtgctggt gctgccggct gagttggcgg
    14101 atcgcggtga gctcgaccac gtgagttact gctcgtgtct gttcgccgac gctcgatcga
    14161 cggtcaagct tccgcagcta cgagctggct cgcagcggcc gcgagcggtc gaacctccgc
    14221 cagccgcgct ggcgagcgga gagtcgacag gggctttgct ccagccgctc gtcaacgtct
    14281 ctccggtcgt gggaacaccc gcgaagaaca gtgaggcgga gcaagttctc tccgagcgac
    14341 tctcacgaga tcaagagctg aagcggttat ttgccttcaa ccaaccggtc gtcacgacgt
    14401 cagggtttcg gccgatcgtg gacttgctct gggcggcggg gaaactggtg atcgagatcg
    14461 atggtgaaga acaccgacgg ctccagaagt tctcccagga ccgcgcacgg gactatgaac
    14521 ttctattgag tgggtactcg gtggttcgat tcaccacctg gaatgtagtg gatcgcactg
    14581 actgggtcgt ggaaagaatt cgtcaagcgg tccgctacct gaatcagcga gagagagcat
    14641 gagactcaat ccgaatccgc agcaaacatt ccttatcctg tcgatgctgt tcggtaagac
    14701 gccggccgag cgggagcccc ggttcagtga tccctccgtg aaagcgctca agtccgtcaa
    14761 gttcagaagg gagctggaag cagcgggcgt ggtccgagta gaaaggagag gtcgctcgag
    14821 tcacctaatt ttggaggatg aagcctggga cttcgccttc aaatacctgc actccgagct
    14881 cccgactaca ccccgtgcgt cgaaagtgct cgaacacgtc ttaggcaaac tgcgactctt
    14941 cctagagtct catgagcact cgctcctgga tcttctcgct ggtgagacgg ccgctgagcc
    15001 caagccccac gtcgaggcaa gctccgcagt catgtcgaat gtgcggacgg cgggtgacac
    15061 ggcggttcga gtggagtcgg acgctacgtg cggcccgccc gactacgaac gagccgtccg
    15121 cgaggcgtgt ctggcactcg cccacgggca gactcgtcgg cgggtgcgtc tcaaggatct
    15181 gcgggagatg gtccgggtcg agcgcacgag tctagatcga acgctggtcg ccatgcaaac
    15241 cgcggggcgt ttggtgctct acaaactaga caacccctcg gagctgtctc ccgaggacga
    15301 cagcgcggcg ctctggattg cgggtcagcc acgtcaccta gtctatttgg aggcatgagc
    15361 atggatgcac tgcagcaagc gctcaacgag gccgactttc agtggaccag ccatatcgac
    15421 agcatctggc gcgattctcc ggcggacatt ccagagctcc agcggagcgt ccgcgagcag
    15481 ctgcggctgc gcctggccgc gctcgcgcgt aaccaagaat cggcgtcacc gctcggagtt
    15541 cctttgctcg ggaccggcgg ttccggcaaa acccacgtgc tgagtgcggt ccggcgcgaa
    15601 gccttcgagt cgaaccagta cttcgtgctc gtggatatga ccgacgtgcg cgagttttgg
    15661 gacacgatgc tgctcggtta cttgcgctct ctcgaccaac agcgtgaagg agagcctcaa
    15721 tttgggcgcc tcctcaaacg cttgctgaag atctccggat ccggttcacc gacctacgag
    15781 cagctgattg ctgctcgccc cccggggttg atcaaccgct gcaacgatct gattcgcaac
    15841 ttggccaagc tggatcgctc gcaaacgcgc gagcaccagg acgtggtacg tgcattggtc
    15901 ctgctcgctt ccgatgactt cgatattcag gagcgcggat atcagtggct tcaaggggtc
    15961 gggatcggcg aggacgaggg cttccaccac gatttcgtgg ttccgcaaag aaagcccgag
    16021 caaatcgtac gcggactttc gtggctgatg aacctggatg cgcccaccgt gctggcgttg
    16081 gaccaattgg acgccatcgt agcggaacac aacctcgcga gcgcgggcgg ccctgattcc
    16141 gagcggagtc cgcggcagct cgcttcgctc tcgatcattc aagggatcgc cggtgggctt
    16201 tcggcgttgc gcgacgtgac ccgccgcacg ctaatcgtcg tgtccagctt ggaagcgact
    16261 tggaccattc tggacgcgcg cgcgccggta accatgaagg atcgcttcga gccggcgttg
    16321 ctgctccagc cgatccggga ggccacatcc attcggcagc tggtggaacg gcgcctggcg
    16381 ggcgcctacg agacgcgcgg agtaac
//
LOCUS       DASTKU010000210         7235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_262127, whole genome shotgun sequence.
ACCESSION   DASTKU010000210 DASTKU010000000
VERSION     DASTKU010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262127"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..492)
                     /locus_tag="VFQ61_07995"
     CDS             complement(223..492)
                     /locus_tag="VFQ61_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954430.1"
                     /translation="MSSDISGAFDAFEAEVSVWAPARMAEVGEVCREVPAMSGVRGEP
                     LLAPGGPELPRPPARHRGQLDDPWSTPKSRVGSRPCVREARKLRP"
     gene            479..3163
                     /locus_tag="VFQ61_08000"
     CDS             479..3163
                     /locus_tag="VFQ61_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9954431.1"
                     /translation="MSELISACALGTNHPTRGDFAPVEPENWAELRTPLGVYTSGEET
                     TFAIYSQNATRVLLEIYDVATGEDAKFDYFMQRGHDDVWRAKLLNVPKGTLYGFRCWG
                     RNWPFSSDFRRGNSVAGFIADVDRDGNRFNPNKLLFDPYARELSHDRETPAMKAQGHH
                     AQMYGSGPELYTGFGHPGVVRRVFDTGRFAPKSVVVQDHTGFGTKPRIEQQNLIIYEV
                     HPRGLTRHPSSQRLRSLVSGIPGFEHVLDVPRELRGSFAGAARAAKYLKALGCNAVEF
                     LPVHEAANSLCPNDEPEVDRTPTEPPHGTYWGYMTYGFFAPDRHYSSDQGLGGPTREF
                     KQMVKAFHDEGIAVILDVVYNHSGEGGIWDNDVRSAEILFLRGLDNAEYYALCGEGKR
                     HYWESTGCGNNLDCSKPAVRRLILDSLAYWTREMGVDGFRFDLATVLGRESANGYDFH
                     SGAELLSEIEQMADRENFLVIAESWDTGSGGYQVGNFPQGWAEWNGMYRDAVRRFLRG
                     DAGQVDAFTQVLNGDYAHFADQGGPHKSINFVTAHDGFTLLDLVSYDQKNNHQPWPFG
                     PSDGGADQNDSWNSDGDHALRRQRMRNFMCVLMFSRGVPMIRGGDEFAQTLNGNNNPY
                     KIDSVATWHNYQMIATSMPTRVNTGGAGRYHDNYGRDANETGKNGFFLFTRALINLRK
                     SHVCLRQDKFADFQMDAGDDVTYQFRREDGRRDLDGSERAVMLRIDGSEVGDHDFLLL
                     VNMHRHAVEFTLPSARPDEPWKRIIDTAAWAEYCGNLFSFEKAEPLPEGTPYSVHGMS
                     IAVFQQVIAPPPPRYPSRPPPRPSQFPAASGQTNVTRSRPSQPPARPSQLPAPPAQSE
                     GSPGFSPASSPQSGAPNPAAEEVSWVQPPPWRPGSRGS"
     gene            3228..3467
                     /gene="rpoZ"
                     /locus_tag="VFQ61_08005"
     CDS             3228..3467
                     /gene="rpoZ"
                     /locus_tag="VFQ61_08005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET9954432.1"
                     /translation="MARVTVEDCLEKEENRFALVVLASTRCRQLMKGVPSFVNTKNKP
                     AVNALREIAAGRVRFHRLTNDVVMEYINELGRVNL"
     gene            3522..4397
                     /locus_tag="VFQ61_08010"
     CDS             3522..4397
                     /locus_tag="VFQ61_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9954433.1"
                     /translation="MTSKKAPRKSVKDPAPAAGSLLKPFTIYDVGYATDVGATAHYAD
                     PRYYSKTYGNRRHDVDYYVQRARLAKGPVLEYGVGNGRIALHMARAGVAVTGVDLSRA
                     MLADFERRLALEPRQVRARVKLEQGDMREVRLERRFPLVIAPFNAILHLYDRRDVEAF
                     LLRVREHLAEDGQFVFDFSIPQPEDLIRDPERAFATTPLEDGETGEPVSYAERFEYDP
                     LRQLLLVRMELEHKNSGRKSTIPLTHRQYFPREMEALLHYNGFREILFTADFTDQPAD
                     RHVDSLVVACRSTPV"
     gene            4491..5426
                     /locus_tag="VFQ61_08015"
     CDS             4491..5426
                     /locus_tag="VFQ61_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET9954434.1"
                     /translation="MAADFYKELGVSRDASADEIKKAYRKLAAQLHPDKNPGDKKAEA
                     RFKAVNRAHQVLSDQEMRKRYDEFGEEGLREGFDPHAARAYQRAAGRGRGRVGADVNV
                     EDLFGAGGGAAGFSDLFGDLFGGGARGVRRPAKGADLQSEIAVDFASALKGTELKLRL
                     QDGGDEVTVRIPPGAGDGDKLRVPGQGAPGPLGGPPGDLLLTIRVRPHASFERSGLDL
                     YLDLPITPKEAFRGAKVRVPTPDGPVTLTVPKHAQSGQVARLKGKGVHRQNQTGDLFV
                     RFQIKLPTDASAEVEKAIETLEAAMTGDVRANISF"
     gene            complement(5460..6614)
                     /gene="add"
                     /locus_tag="VFQ61_08020"
     CDS             complement(5460..6614)
                     /gene="add"
                     /locus_tag="VFQ61_08020"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET9954435.1"
                     /translation="MASPEISADSQVAVPIEVFRALPKADLHVHLDGSLRLSTILEIA
                     RKERIELPANDEHALSQAIGCGKHFGSLVDYLKGFDITLRVMQTEEALERIAFELAED
                     AHLENVRYMEVRYAPMLHTRRGLKLTRVVEAVLQGLRRARETHGIKANVIVCGIRNIS
                     PESSYEMAELAVAYKGRGVVGFDLAGAEADFPAKHHRAAFQLVRDNNINCTIHAGEAY
                     GPESIAQAIHVCGAHRIGHGCRLRENGDLLHYLNDHRIPLECCPSSNVQTGAVRDLRS
                     HPLKLYFDLGLRVTVNTDNRLITDTSVSKELYLVHSMMGVPFSDIKSMIVAGFKSSFL
                     PFHERNSLVRKVVAELSRYDDHGALREGAPVALELNSRRVVLSTAHAEAE"
     gene            6686..>7235
                     /locus_tag="VFQ61_08025"
     CDS             6686..>7235
                     /locus_tag="VFQ61_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954436.1"
                     /translation="MRREPTWLLNILRPVTRVEDGEAVTALLLTLNVFLLLSAYYIIK
                     PVREALILAMRSGAEYKSYMSGVIAILLLFAVPAYAKIVDRLPRLKLVVGVTLFFAAN
                     LLLFTVAAEVPALRERLGLIFYAWVGVFNMMVVAQFWSFANDLYDEEQGERLFPMVAL
                     GGSLGAALGSKLSAGLIPLLGLR"
BASE COUNT         1370 a         2367 c         2222 g         1276 t
ORIGIN      
        1 gcaactccga gaccacatcg cttcctacat cgccgggtgg aacaaggacc cgacgccctt
       61 catctggacc aagcccgctg ccgctatcat ccgcagccac cgccgaatgc ttgatcgcat
      121 ctcacatgcg gtgcactagt gcagcaccgg aaaacgttag cgcagcgccc gccaagcata
      181 acgcagcacc cgaaagccgc tgcacagcgg ttccagacgc gatcacggac gcagctttcg
      241 agcctcgcgc acgcacgggc gcgaccctac acgcgacttc ggcgtagacc aagggtcgtc
      301 gagttgcccg cggtggcgcg cgggcggcct gggcagttcc gggccacccg gggccagcag
      361 gggctcgcct cgaacacccg acatggcggg cacctctcga catacctcac ccacttcggc
      421 cattcttgca ggtgcccaaa cagaaacttc cgcctcgaat gcgtcgaacg cccctgagat
      481 gtccgagctc atcagcgcat gtgccctagg aacgaaccac ccgacacgag gtgatttcgc
      541 gcccgtcgag cccgagaatt gggccgaact gagaacccct ctgggtgtgt acacgagtgg
      601 tgaggagacc actttcgcga tttattcgca aaatgcgacg cgcgtgctgc tcgagatcta
      661 cgacgtcgcg accggcgagg acgccaagtt cgattacttc atgcagcgtg gccacgacga
      721 cgtttggcgc gccaagctcc tgaacgtacc caagggtacg ctttacggct tccggtgttg
      781 gggacggaac tggcctttct cgtccgactt tcgacgaggt aacagcgtcg cagggttcat
      841 cgccgatgtg gatcgcgacg gcaatcgctt caatcccaac aagctgttgt tcgatcccta
      901 tgcgcgggag ctctcgcacg accgcgaaac cccggccatg aaagcccagg gccaccacgc
      961 ccaaatgtat ggctcggggc cggagttgta tacgggcttc ggtcaccccg gtgtggtgag
     1021 gcgcgtattc gacaccggac gtttcgcgcc aaaatccgtc gtggtgcagg accacacggg
     1081 ctttggtacc aagccacgca tcgagcagca aaacctgatc atttacgagg tgcatccgcg
     1141 cggcttgacc cgacacccat cctcccagcg cctgcgcagt ctggtgtccg gcattcccgg
     1201 cttcgagcac gtcctcgatg tgcctcgcga gctccgcggc agcttcgcgg gagcggcgcg
     1261 ggccgccaag tacctcaaag ccctcggctg caacgcggtg gagttcctac cggttcacga
     1321 agcagcaaac tccttgtgcc cgaacgacga gcccgaggtg gaccgcacgc cgaccgaacc
     1381 cccgcacggc acgtactggg gttacatgac gtacggcttc tttgcgccgg atcgtcacta
     1441 ctcttcggat cagggcctcg gcgggcccac ccgtgaattc aagcaaatgg taaaggcctt
     1501 ccacgacgaa ggcatcgccg tcatcctcga cgtcgtctac aaccatagcg gggaaggcgg
     1561 gatctgggac aacgacgtgc gctccgccga gatcctattc ctgaggggcc tcgacaacgc
     1621 cgagtactac gcgctgtgcg gcgagggcaa acgtcactat tgggagagca ccggctgcgg
     1681 caacaatctc gattgttcga agcctgccgt ccggcgcctc atcctcgact cgctcgcgta
     1741 ttggacccgc gaaatgggcg tagatggctt tcgcttcgac ttggccacgg tgctcggccg
     1801 cgaatccgcc aatggctatg actttcactc gggtgccgag ttgttatccg agatcgagca
     1861 gatggccgat cgggagaact tcttggtgat cgcggagtct tgggacaccg gctccggcgg
     1921 ctaccaagtc ggcaatttcc cgcagggctg ggcggaatgg aacggcatgt atcgagatgc
     1981 ggtgcggcga ttcctgcgcg gtgacgcggg ccaagtggat gccttcaccc aggtactcaa
     2041 cggcgactac gcgcacttcg cagaccaagg cggcccccac aaatccatca acttcgtgac
     2101 cgcgcacgac ggcttcacgt tgctggattt ggtgagctac gaccaaaaga acaaccatca
     2161 accctggccg ttcgggccga gcgacggcgg cgccgatcag aacgactcgt ggaactcgga
     2221 tggcgaccac gcgctgcgcc gccagcgcat gcggaacttc atgtgcgtgc tgatgttctc
     2281 gcgcggcgta ccgatgatca gaggcggaga cgagttcgcg cagacgctga acggtaacaa
     2341 caacccgtac aagatcgaca gcgtcgcgac ctggcacaac tatcagatga tcgcgacatc
     2401 gatgccaacc cgagtgaaca cgggcggcgc tggtcgctat cacgacaact acggtcggga
     2461 cgccaacgag accggcaaga acggcttctt tctgttcaca cgcgccttga tcaacctgcg
     2521 caaaagccac gtctgtttgc ggcaagacaa gttcgctgac ttccaaatgg acgccgggga
     2581 cgacgtcact taccaattca gacgcgaaga cgggcgtcgc gacttggacg gctccgagcg
     2641 cgcggtcatg ctgcggatcg acggcagcga agtcggcgat cacgacttcc tgctgctcgt
     2701 gaacatgcac agacacgccg tcgagttcac gctcccgagc gcgcgccccg acgagccctg
     2761 gaaacgcatc atcgataccg ccgcgtgggc ggagtactgc ggcaacctgt tctccttcga
     2821 aaaggccgag ccattgccgg agggcacccc ctactcggtg cacggcatgt ccatcgccgt
     2881 attccagcaa gtcatcgcgc ctccaccacc gcgctacccc tctcggccac cgccgcgacc
     2941 gtcccaattt ccggcggcca gtggccagac gaacgtcacg cgctcgcggc cctcgcaacc
     3001 tcccgcccgg ccctcgcagc tgcctgcgcc cccagcgcag tctgaaggca gccccggctt
     3061 ttcccccgct agttcacctc aatccggtgc gcccaacccg gcagccgagg aggtgtcgtg
     3121 ggttcaacct ccgccatggc ggcccggcag ccggggatcg tgatcttgcg cgagtaggat
     3181 cccggcgtta cggtgtcgac cccctttcga acgaggcatc atcccgaatg gcgcgtgtaa
     3241 ccgtagaaga ttgtctggag aaggaagaga accgcttcgc cctggtggtg ctcgcgtcga
     3301 cgcggtgccg gcaattgatg aaaggtgttc cgtcattcgt gaacaccaag aacaaacccg
     3361 cagtgaacgc gctgcgcgaa atcgcggctg gccgggtgcg ttttcatcgg ctcaccaacg
     3421 acgtggtgat ggagtacatc aacgagctcg gtcgcgtgaa cttgtaaacg gccgcgagcc
     3481 ctagcttcgc atgaggggct cttcggcgtc cgcgacacga aatgacctcg aagaaagccc
     3541 ctcgcaagag cgtgaaggac ccagcgcccg ccgcgggttc actgctgaag ccgttcacga
     3601 tttacgacgt tggttacgcg acggacgtgg gcgccaccgc gcactacgcc gaccctcgtt
     3661 actactccaa gacgtacggt aaccgtcgtc acgacgtcga ctattacgtt cagcgcgcgc
     3721 gcctggcgaa aggccccgtg ctcgagtacg gcgtcggaaa cgggcggatt gctctgcaca
     3781 tggcccgcgc cggagttgcc gtaactgggg tcgacctatc gcgtgccatg ctggccgatt
     3841 tcgagcgccg actggcgctg gaaccgcggc aggtgcgagc gcgcgtgaag ctggaacagg
     3901 gggacatgcg cgaggtgcgg ctcgaacggc ggttcccact cgtgatcgcc ccgttcaacg
     3961 ccatcctcca cctgtacgat cggcgcgacg tcgaggcctt cctactgcgg gtgcgcgagc
     4021 atctcgcaga agatgggcag ttcgtattcg acttttcgat cccgcagccc gaagatttga
     4081 tccgcgaccc cgagcgagcg ttcgccacga ccccgctcga agacggcgag accggcgagc
     4141 ccgtctctta cgcggagcga ttcgagtacg atccgctgcg ccagctgctg ctcgtgcgca
     4201 tggagctcga gcacaagaat agcggccgca agagcacgat cccgcttacc catcgtcagt
     4261 actttccgcg ggaaatggag gccttgctcc actacaacgg gtttcgggaa atcctcttca
     4321 ccgccgattt cacggatcaa cccgccgatc ggcacgtgga ttccctggtc gtcgcgtgcc
     4381 gaagcacgcc ggtgtgagct aaccggccac tcacgcgaac ggtccggtcc ggaggatgac
     4441 gggggccttg atcggcagcg cgacgctgct tagcttgccc aggagacgcc atggctgccg
     4501 acttctacaa ggaattggga gtatctcggg acgcctcggc ggacgagatc aagaaggctt
     4561 atcgaaagct cgccgctcag cttcacccgg acaagaaccc cggcgacaag aaggccgagg
     4621 cccgcttcaa agccgtaaac cgcgcgcatc aggtgctcag cgaccaggag atgcgcaagc
     4681 ggtacgacga gttcggcgaa gagggcctgc gagaaggctt cgatcctcac gcggcgcggg
     4741 cttaccaacg cgcggctggg cgcgggcgcg ggcgagtcgg cgccgacgtc aacgtggagg
     4801 acttgttcgg ggcgggtgga ggcgccgctg gcttttcgga cttgttcggc gatttgttcg
     4861 gtggcggcgc gcgcggtgtg cgacgccccg ccaaaggtgc cgacctccag agcgaaatcg
     4921 cggtcgactt cgcctcggca ctgaagggca cggagctaaa gctccgcctt caagacggcg
     4981 gtgacgaggt caccgttcgc atccctccgg gcgccggtga cggggacaaa ctacgggtgc
     5041 caggacaggg tgcgccgggc ccgctcggag gcccgcctgg agatctcctg ctcaccattc
     5101 gggtgcgccc ccacgcctcc ttcgagcgtt cgggtctgga cttgtacttg gatctgccca
     5161 tcacgcccaa agaagcgttc cgcggcgcga aggtgcgcgt ccccactccg gacgggcctg
     5221 tcacgctgac ggtccccaag cacgcccaaa gcggtcaggt cgcgcgcctc aaaggcaagg
     5281 gcgtacaccg ccagaaccaa accggcgact tgttcgtgcg cttccaaatc aagctgccga
     5341 cggatgcttc cgccgaagtc gagaaagcca tcgagacgct cgaggcggcc atgaccggcg
     5401 acgttcgtgc gaacatcagc ttctgaacgc gccggcaaga ctgcggttcg cctcgtctcc
     5461 tattcggctt cggcgtgtgc ggtcgaaagc accacccgac ggctgttgag ctcgagcgcc
     5521 accggagcgc cctcgcgcaa agcgccgtga tcgtcgtaac gcgacaactc ggcgaccacc
     5581 ttccgaacca acgagttacg ctcgtggaac gggaggaagc tcgacttgaa gcccgccacg
     5641 atcatggact tgatgtccga gaagggcact cccatcatgc tgtgcaccaa gtagagctcc
     5701 ttggagacac tggtgtcagt gatcaggcga ttgtccgtgt tcaccgtcac cctcagcccc
     5761 agatcgaaat agagcttcag cgggtggctc cgcaggtccc gaacggcgcc ggtctgcacg
     5821 ttgctcgagg ggcagcactc cagagggatg cggtggtcgt tcagataatg gagcaggtcg
     5881 ccgttctcac gcagccggca gccgtgaccg atgcggtgcg cgccgcacac gtgaatcgcc
     5941 tgcgcaatcg actccgggcc ataagcctcg ccggcgtgga tggtgcagtt gatgttgttg
     6001 tcgcgaacca gctggaacgc cgcgcggtgg tgcttcgccg ggaaatcggc ctcggcgccg
     6061 gccaggtcga acccgaccac gccgcgccct ttgtatgcca ctgctagctc cgccatctcg
     6121 taggaagatt caggtgagat gttgcggatc ccgcacacga tgacgttggc tttgatgcca
     6181 tgagtttccc gggcgcggcg caggccctgc agcacggcct ccacgacccg cgtgagcttc
     6241 aagccgcgtc gggtatggag catcggcgcg tagcgcacct ccatgtaccg cacgttttcc
     6301 aggtgcgcgt cctccgccag ctcgaacgcg atgcgctcca gagcttcctc cgtctgcatc
     6361 acgcgcagcg tgatatcgaa gcccttcaga tagtccacca gcgagccgaa gtgcttgccg
     6421 cacccgatcg cttgggagag cgcatgctca tcgttggcgg gcaactcgat ccgctctttg
     6481 cgagcgattt ccaagatggt gctcaggcga agcgagccgt ccaaatgaac gtgcagatcc
     6541 gccttgggga gcgcgcggaa gacctcgatg ggaactgcaa cctggctgtc agcactgatt
     6601 tccgggctag ccatggcgaa ctgtaccacg ccgcagcgcc acgagccctt gcgcccctag
     6661 ccgagctgta agatcaatcc gccgcatgcg acgcgaaccg acttggctgc tgaacatcct
     6721 gcgccccgtc actcgggtcg aagatggcga agcagtgact gcgctgctcc tgacgctaaa
     6781 tgtcttcctc ctgctctccg cctactacat catcaagccg gtccgcgaag cgctcatcct
     6841 ggcgatgcgc tcgggcgctg aatacaaatc ctatatgagt ggcgtgatcg cgattctgct
     6901 gctctttgcc gtccccgcgt acgccaagat cgtagaccgc ctgccgcgcc tgaaactggt
     6961 ggtcggggta accctattct ttgccgcgaa tctgctgctt ttcacggtgg ccgccgaagt
     7021 gccagccctc cgcgagcggc tcgggctgat cttctacgcc tgggtcgggg tcttcaatat
     7081 gatggtcgtc gcgcagtttt ggtctttcgc gaacgacctg tacgacgaag agcagggcga
     7141 acgattgttt ccgatggtcg ccttgggcgg ttcgctcggc gctgcgctgg ggagcaagct
     7201 gtctgcgggg ttgattccgc tgctcggctt gcgag
//
LOCUS       DASTKU010000211         5826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_708826, whole genome shotgun sequence.
ACCESSION   DASTKU010000211 DASTKU010000000
VERSION     DASTKU010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708826"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VFQ61_08030"
     CDS             complement(<1..1131)
                     /locus_tag="VFQ61_08030"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HET9954437.1"
                     /translation="MLVSSAAIAVALATTPEAEACGGFFCSAVQPVNQAAERIIFSNN
                     GDGTVTAVIQILYEGPSEKFSWLLPISTVPKQDSDIGVASNLAFQRLQSATNPQYNLN
                     TRLEGTCKQLAVPGAANAGGGGRAAAASGGSTSGPPPDVVVEASGVVGAFEWTVISIN
                     ESTADPSDAATKWLTDNGYDVPEGAKGLLRPYLEDGLYLLALRLTKGSTTGSIRPISL
                     TYEASKPMIPIKLTAVAANQNMGVMTWLLSTARGVPQNYYSLELNEARINWFNANSNY
                     NDVVNEAADSASGHGFVTEMAGSTERLKNVVWTTFEDTNWSSFRSGTFQNLGQLYSSA
                     LSQWGSFDGFDDAARSTIQLPDGVQYSDLRTCPSCYQDQIKFS"
     gene            1403..1957
                     /locus_tag="VFQ61_08035"
     CDS             1403..1957
                     /locus_tag="VFQ61_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954438.1"
                     /translation="MQRISMFVLARAWVLAASCVLLPACGSSEAPPTDVPVEPDPEPE
                     PVPEPKVLIGVPAGEDELSFAELAADSELRLQTFGQGGTHVMLGVRCIGFGQRVFVDI
                     EIENTRTGAISVTPAPVRAQLLYCDVDENTCDLVPILGMTSGLTEPGEDRDGLPIEIR
                     VRVHNAAGLTGEAHQTAVLSTADL"
     gene            2110..4914
                     /locus_tag="VFQ61_08040"
     CDS             2110..4914
                     /locus_tag="VFQ61_08040"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HET9954439.1"
                     /translation="MTRQLTSLALISFMVAGCADGDFVAPPAEPATWSQVEQSQGAAL
                     LSVHGTSDRDVWFSGADDGTGPLLVHYDGTSFQRIPSGVRGDLWWTHATVPGGPVFFG
                     GADANLLRYQDGRFERLTTPGLGKHTVYGVWAASSTDVYAVGSAAGRNGFIWHYDGQS
                     FRDLALPADMPQNDKHDVPGLFKVWGTSARDVWVVGGNGVVLRGNAERGFQRLPSMSQ
                     STLFTVHAHDGRVVIVGGGDGGVLFEAEGDEIRELTPAASPLLQGACVANDGAVWAVG
                     MGGSIYESRGGSFEPVDPGIDFTATQSLHATWVDPSGGVWAVGGNVLTPKLDAGIAIH
                     RGVGLPRFDVPAPVIPAAVCPESAIDPAPHGSIARRWNEQILGAIRRDLPRPTVHARN
                     LFHSSVAIWDAWAAYDSRAEGYLVSEHHTARDVQAAREEAISYAAYRVLSHRYASAIG
                     GAISQACFDAFMQRLGYDPADTGVEGDSPRAFGNRVGMAVVERFAEDGANEADNYRST
                     QSFSNGVPNLVVDLPGTDTQNPILWQKLVLAKAATQNGIPTDAGAQDYIGAHWGGVEP
                     FAIERPAPDEPYLDIGKAPLEADDDLVDAALEVLRKSSELDSEDHVSIDISPGAYGNN
                     SLGTNDGAGHRRNPVTGRAYASQRVKRGDFTRVLAEFWADGPNSETPPGHWNTLANGV
                     ADSDGFERRLFGEGEPLSALDWDVHLYLALNGAEHDAAIAAWELKRKYATARPITLIR
                     YFGSLGQRSDPSLPSYDPRGLPLVEGLVELITPQSSAPGGRHAHLARYVGELAVRSFR
                     GEPGDRVRELAGVGWVRVQDWMPYQRRTFVTPAFPGYVSGHSTFSRAAAVVLSELTGS
                     EYFPGGLAGYTVNPGGLTFERGPTAAVRLEWATYFDAADQAGQSRLWGGIHIRNDDFD
                     GRKIGARVGERAVARARKFF"
     gene            complement(4968..>5826)
                     /locus_tag="VFQ61_08045"
     CDS             complement(4968..>5826)
                     /locus_tag="VFQ61_08045"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9954440.1"
                     /translation="YLNPIERDGDSERRAELRARMQPFILRRTKQQVLQDLPSLTVIR
                     HEVRLDEQEQLRYALLRRQIHDKLFTPHGRRQYKIEVFAELVRLRRFCCHPRLVFPDA
                     TLESSKLRVLLDLVEELRENGHRALVFSQFVDFLDLVREVLNERGIRYEYLDGSTPKE
                     QRQARVAAFQNGTAELFLISLKAGGFGLNLTGADYVIHLDPWWNPAVEAQATDRAHRI
                     GQLRPVTVYRLVTKDTIEENMVALYADKRALADGILEDTDLAARLDFDDVSRLLGEHP
                     PQTLSDANL"
BASE COUNT         1027 a         1744 c         1900 g         1155 t
ORIGIN      
        1 ggagaacttg atttgatctt gataacagga tgggcaagtg cgcaggtcgc tgtactgaac
       61 tccatccggt agctgaatgg tcgagcgcgc ggcgtcatcg aagccgtcga agctgcccca
      121 ttgactgaga gccgagctgt atagctgccc cagattttga aacgtgccgc ttcggaacga
      181 gctccaattc gtatcctcga atgtggtcca aaccacgttc ttcaggcgtt cggtcgatcc
      241 cgccatctcg gtgacgaacc cgtggccaga agccgagtcg gcggcttcgt tcactacgtc
      301 gttgtagtta ctgttggcat tgaaccaatt gatgcgggct tcgttcagct cgagagagta
      361 atagttctgc ggcacgccgc gcgcggtgga cagcagccaa gtcatcaccc ccatgttctg
      421 attggccgca accgccgtga gcttgattgg aatcatcggc ttggaggctt cgtaggtcag
      481 cgaaatcggc cgaatcgagc ctgtggtcga gcccttggtc agtcgcagag cgagcagata
      541 caagccgtcc tcgaggtaag gccgcagcaa ccctttcgct ccttctggta cgtcatagcc
      601 gttgtccgta agccacttgg tggcggcatc cgaaggatcg gccgtgctct cattgatcga
      661 gatgacggtc cactcgaaag ctcccaccac cccgctcgct tcgacgacga cgtccggcgg
      721 agggccgctc gtcgagcctc ccgatgcggc tgccgctctg cctcctccgc cagcattggc
      781 ggccccggga acggcaagct gtttacacgt accttccagc cgtgtgttca ggttgtactg
      841 gggattcgtt gcggattgca acctttggaa tgccaggttc gaggctacgc caatatccga
      901 gtcctgcttc ggaaccgtag atatgggcag cagccaagag aacttctccg agggcccttc
      961 gtagagaatt tggatgacgg cggtgaccgt cccatctccg ttgttcgaaa atatgatgcg
     1021 ctctgccgct tgattgacgg gttggaccgc ggagcagaag aaccctccgc acgcttctgc
     1081 ttctggcgtg gtggcgagtg ctaccgcgat agcagcgctc gagacgagca tccgcctcca
     1141 gttctttcgg gctttccagg ttgtcgtcaa aacatgagtg gagtcgggtt gcgacatttc
     1201 gaagtctcct gcgccaggtc aatcgaggga cgaccttccg caccctcacg accgagctct
     1261 gcggggaagt gtctggcaca atcaggcaca attccgagtg gaccggcgta atactgcaag
     1321 tcccaagcca gccttgtctt ggcttcagtg agcgcggcgg acacccgggc cggaagcttg
     1381 cgcggagcat acgagtgcta tcttgcagcg tatctcgatg ttcgtgttag cgcgtgcgtg
     1441 ggtgttggcg gcgagttgcg tgctcttacc ggcgtgcggc tcttcggagg cgccacctac
     1501 ggacgtcccc gtggagccgg atcccgagcc tgagccggtg ccggaaccca aggttctcat
     1561 cggggttcct gccggtgaag acgagctgag cttcgctgaa ctcgccgccg attctgaact
     1621 gcgcctgcaa acttttggac aaggtggcac tcacgtcatg ctcggtgtcc gatgcattgg
     1681 attcgggcag cgcgtcttcg tggatatcga gatcgagaac acgcgaacgg gcgccatctc
     1741 cgttacgccg gcgcccgttc gcgcgcagct tctgtactgc gatgtcgatg agaacacctg
     1801 tgatctggtg ccgatcttgg gcatgacctc ggggctaacg gagcccggag aggatcggga
     1861 cggcttaccg atcgagatcc gcgtccgcgt tcacaatgcg gcggggttga cgggggaagc
     1921 tcaccagacg gcggttctca gcaccgccga tctgtagagc gacgggggtg gcgcaactcg
     1981 gcacagaagc tcgcacacga tcctaggctc gcggcggcca atcgcaaatt gctcgaggat
     2041 tgcactatgc acggatattg cagaccatga agccgtggaa ttgtggattt cggagagggt
     2101 tcgcccaacg tgacccggca actcacatct ttggctttga tttcgtttat ggtcgcgggg
     2161 tgcgcggatg gggacttcgt cgcgccgccc gcggaacctg cgacttggtc ccaagtcgag
     2221 cagagccagg gcgcggcgtt gctgagcgtg cacggtacgt cggatcgcga tgtctggttt
     2281 tccggcgccg acgacggcac cgggccgttg ctcgtgcact atgacggaac ctcattccaa
     2341 cggatcccga gcggtgtgcg cggcgatctg tggtggaccc atgccaccgt gcccggcggt
     2401 cccgtcttct tcggcggtgc cgacgcgaac ttgttgcgat accaagacgg tcgcttcgag
     2461 agactgacta cgccggggct gggtaagcac accgtgtatg gggtgtgggc ggcctcgtcc
     2521 accgacgtct atgcggtggg ttcggcggcg ggccgcaacg gattcatctg gcactacgac
     2581 ggtcagtcct tccgagacct ggctttgccg gcggacatgc cgcaaaacga caaacacgat
     2641 gtcccgggct tgttcaaggt ctggggtacc tcggcgcgcg acgtgtgggt ggtgggcggc
     2701 aatggggtcg tgttgcgcgg caacgcggaa cgcggttttc agcgcctgcc aagcatgagc
     2761 cagagtacgt tgttcacggt gcacgcccac gacggacgag tcgtgatcgt cggcggcggg
     2821 gatgggggcg tgctgttcga agcggaggga gacgagattc gggagctcac gccggcggct
     2881 tcgccgctct tgcaaggtgc ttgtgtcgcg aacgacggcg cagtgtgggc ggttggcatg
     2941 ggaggatcga tttacgagag ccgggggggc agcttcgagc ctgtcgatcc gggaattgac
     3001 ttcaccgcga ctcaatcgct tcacgccacg tgggtcgacc cgagcggtgg ggtttgggca
     3061 gtgggtggaa atgtgctcac acccaagctc gatgccggaa ttgccattca ccgcggcgtg
     3121 gggctgccac gcttcgatgt gcctgcgccc gtcatccccg cggcggtgtg cccagagtcg
     3181 gccatcgatc cggctcccca cggctcgatt gcgcggcgtt ggaacgagca aatcctcggc
     3241 gcgatccgcc gcgacttgcc gcgtccgacc gtgcacgccc ggaatctctt tcactcgtcg
     3301 gtggcgatct gggatgcctg ggccgcttac gactcgcgcg cggagggcta tctcgtttcc
     3361 gagcatcaca cggctcgtga cgtgcaggcc gcgcgcgagg aggccatttc ctatgcagcg
     3421 tatcgcgtgc tgagtcatcg ctatgcgagc gcgatcggag gagcaatttc gcaggcctgc
     3481 ttcgacgcgt tcatgcagcg gctcggctac gatccggccg acaccggggt ggagggagac
     3541 tcgccgcggg cctttggcaa tcgagtgggc atggcggtgg tagagcgttt tgccgaagat
     3601 ggcgccaacg aggcggacaa ttaccgatcg actcagagtt tctcgaacgg cgttccgaac
     3661 ctggtcgtgg acttgcccgg caccgacacc cagaacccga tattgtggca aaaactggtg
     3721 ttggcaaagg ccgctacgca gaacggcatt cctaccgatg cgggcgccca ggactatatc
     3781 ggcgcgcatt ggggaggggt cgagccgttc gccatcgagc gccccgcgcc ggatgagccc
     3841 tacctcgata tcggcaaggc tccgctcgaa gccgatgacg acttggtcga cgcagcgctg
     3901 gaggtattgc gcaagagctc cgagctcgat tccgaagacc acgtctcaat cgatatctcc
     3961 ccgggcgctt atggcaacaa ctcgttgggc acgaacgacg gcgcgggcca tcgccgcaac
     4021 ccggtcacgg gtcgagccta cgcgtcacaa cgagtcaaac gaggtgactt cacgcgcgtg
     4081 ttggccgagt tttgggcgga cggtccgaac tcggagaccc cacccggaca ttggaatacg
     4141 ctggccaatg gcgtcgcgga cagcgacggc ttcgaacgtc ggctgttcgg ggaaggggag
     4201 ccgctctcgg ctctcgattg ggacgtgcat ctctatctgg cgttgaacgg cgcggagcac
     4261 gacgccgcca ttgccgcgtg ggagctcaag cgcaagtacg cgacggcccg gcccatcacg
     4321 ctgattcgat atttcggcag cctcggccag cgcagcgatc cgagcttgcc gtcttacgac
     4381 ccgcgcggtt tgccgctcgt cgagggtttg gtcgagctga tcacgcccca gagctcagcg
     4441 cccggaggcc gccacgcgca tttggcccgt tacgtggggg agctcgccgt gcgatcgttt
     4501 cgcggggaac cgggggaccg cgtgcgcgaa ctcgcgggcg tcggttgggt gcgagttcag
     4561 gattggatgc cttaccagcg acgcaccttc gtgacgcccg cgtttccagg ctatgtatcg
     4621 ggccacagca cgttcagtcg cgcggccgcg gtggtattga gtgagctcac gggcagtgag
     4681 tatttccctg gtggactcgc gggatacacc gtgaatcccg gcggactgac gttcgagcgg
     4741 ggacccacgg cggcggtgcg gctcgaatgg gctacctact tcgacgccgc cgatcaagcg
     4801 ggtcaatcgc ggctctgggg tggcattcac atccgtaacg atgatttcga cgggcgtaag
     4861 attggtgcgc gcgtcgggga gcgcgccgtg gcccgcgccc gtaagttctt ctgagcccgc
     4921 tcgattccgc ctggatctcg cgcgaaattc aggctgagtg cggtccgtca aagattggcg
     4981 tcgctgagcg tctggggtgg gtgttccccg agcaggcggg agacatcatc gaagtcgagg
     5041 cgcgcggcca agtcggtatc ctccaaaatg ccatccgcca gcgcccgctt gtcggcgtag
     5101 agcgcgacca tgttttcttc gatcgtatct ttggtcacga gccgatacac cgtcacgggt
     5161 cgcagctggc caatccggtg agcgcgatcc gtggcttggg cctcgacggc tggattccac
     5221 cacggatcga ggtgaattac gtagtcggcg ccagtcaaat tcagcccgaa ccctcccgct
     5281 ttcaagctaa tcaagaacaa ctcggccgtg ccgttctgga aagccgccac ccgagcctgc
     5341 cgctgttctt tcggcgtcga gccgtccagg tattcatagc gaatcccgcg ttcattcagc
     5401 acctcacgga ccaagtccaa gaagtctacg aattggctga ataccaacgc gcggtgccca
     5461 ttttcgcgta gctcttccac caagtccaag agcacccgca gcttcgaaga ttccagggtt
     5521 gcgtccggaa acaccaggcg cgggtggcag cagaatctgc gtagccgcac gagctcggca
     5581 aaaacttcga ttttgtactg gcggcgaccg tgcggggtga acaacttgtc gtgaatctgg
     5641 cggcgcagca gcgcgtaccg cagctgctcc tgctcatcca gccgcacctc gtgccgaatc
     5701 acggtaaggc tcggcagatc ctgcaggacc tgctgcttgg tgcgccgcaa aatgaagggc
     5761 tgcatgcgtg cgcgcagttc ggcgcggcgt tcgctgtccc cgtcccgttc gatcggattg
     5821 agatag
//
LOCUS       DASTKU010000212         4789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_262466, whole genome shotgun sequence.
ACCESSION   DASTKU010000212 DASTKU010000000
VERSION     DASTKU010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262466"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VFQ61_08050"
     CDS             complement(<1..976)
                     /locus_tag="VFQ61_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HET9954441.1"
                     /translation="MPLKVLILSRTSGAYSTQRLKQACVKRGHVPRVFNTLGFSLLLE
                     NRGLELFYRGRLVSQYDAVIPRIGASISGFGSAVLRHLEQKGVFALNSSQSIAASRDK
                     LRALQILAGHELPIPATAFVRDRSSVKEAIAQVGGPPIIIKLIEGSQGVGVILADTAK
                     TAEAILETLLIARQCVVVQKYISESRGTDLRALVVGQTVVAAMRRAAAPQEFRTNVHR
                     GGTVEKVRLTREQERVAIDATQTLGLRVAGVDLIESDEGPLVLEVNSSPGLEGIEAAT
                     GVDVASSIVEHLEEQIQFPVFDVRQRLTRGGEHDLLELGIQPKSPWVGQ"
     gene            complement(1025..1486)
                     /locus_tag="VFQ61_08055"
     CDS             complement(1025..1486)
                     /locus_tag="VFQ61_08055"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HET9954442.1"
                     /translation="MADPDRLIIGVTEYVDIPEWNVLQLRAKVDTGARSSALHVENLC
                     EISRDRVRFEVRLHRYKLERRLTIEAPITRRCRVRASTGVASARVFVSVGLRIGPLVQ
                     NIELGLVDRANMLYRMLLGRTALGRYFLIDPAQRYALTSPHGDFPARGLRR"
     gene            1576..2337
                     /locus_tag="VFQ61_08060"
     CDS             1576..2337
                     /locus_tag="VFQ61_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954443.1"
                     /translation="MADEFPTLEIVLAACVAGLAWRGFRLFWAERSLAAQVEKLNPDL
                     PNQGLTGGNVERASAYLGVLLRAAGEEQARAESQAQPRASARTAPTPSEKPSGPPRAH
                     VAEIVHERALRMRRRLQGASARDLLGCAVLGGAAMYAGAADLGVAKTLFVLGGLGSGL
                     LLLSVLLRMRLDSRVELAEKAVIASFRSGSRAARSFSSRPAADRCPACGQPGIVHHET
                     AAELGSHLSALGLNELVRCPSCGHIEGRIRPVTVG"
     gene            2404..3144
                     /locus_tag="VFQ61_08065"
     CDS             2404..3144
                     /locus_tag="VFQ61_08065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9954444.1"
                     /translation="MDASGSHASNRSYYDAFSERYEAQRGPNDPGGYHDLVDELEAEF
                     VRRYGNGADVLEVGCGTGLLLERIQRFARSVKGVDLSPGMLAKARERGHDVLEASATD
                     LPFADQSFDVTCSFKVLAHVKEIERALSEMARVTRVGGYVIAEFYNPYSMRALLKRLA
                     PPGKVADGTHERHVYTRFDSPRAIARLVPSNCRWVASRGVRIATPTAHVMRHRVGNAV
                     FRKLERLLCDSPLRALGGFYVVALERVR"
     gene            3150..4061
                     /locus_tag="VFQ61_08070"
     CDS             3150..4061
                     /locus_tag="VFQ61_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9954445.1"
                     /translation="MSAVPQTERPAAWWLATLKRRAQRQSDEYVDLSQPITWPSPEAQ
                     RAAIKFFNAAFRAEESGLRQAHELAHEVASWDPELAEVLRLYGNEEGWHRELLTEFLD
                     YIGGRVEPMGRVTRTLYSVYARAHRMESIVLTNLMFECIGSTTYRLTLGRVEHPALRK
                     MLTILTRDEAFHVPLNVFFLRQVLARSGRLEKLRVEYLYKLLFLALVLLPIASRRKAL
                     AFDGIPTLELMRAYARALGGLFPEGSFPKGSHLSMRPPRLALWALGLRSRDLQGDELL
                     QIGMDAAERAADRDQVAVRGLDFKNAP"
     gene            complement(4045..>4789)
                     /locus_tag="VFQ61_08075"
     CDS             complement(4045..>4789)
                     /locus_tag="VFQ61_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954446.1"
                     /translation="VESARIYHAACLIGAGQPEKADEPLKAAIRANVQMRPPDSLVFP
                     PPVIERFLRVRQTLYDEIKRAEARRVQEAREAAEARAARERAEAERVAELERLAAREV
                     LVVKNRRFIAWIPFGAGQFQNGDEGLGWAFFTGELMLSATALTSLGFQTYLAREASQL
                     RNEENNQVLRTWNAALNVSSYALIGVAAIGVLQAHLAFVPEVKQERVHPLPERLRRPH
                     VPPAPTPVTIVPDVGFQSDRFTLGVTGRF"
BASE COUNT          839 a         1545 c         1525 g          880 t
ORIGIN      
        1 attgtccaac ccaaggagac ttgggctgta tccctagttc gagcaaatcg tgttcgccgc
       61 cgcgcgtgag gcgctggcgc acatcgaaca ctggaaactg aatctgctct tccaggtgtt
      121 cgacaatcga agacgccaca tcgaccccgg tagcagcttc tatgccctcg agaccaggcg
      181 acgaattcac ctcgagcacc agcgggcctt cgtcactttc aatcaagtcc acgccagcca
      241 cgcgcaaccc cagtgtctgc gtagcgtcga tggccacgcg ctcttgctcg cgtgtgagcc
      301 gcactttctc tacggtcccc ccgcgatgca cgttggtgcg gaactcctgg ggtgcggccg
      361 ccctgcgcat ggcagccacg accgtctgac ccacgaccag cgcgcgcaag tccgttccac
      421 ggctctctga gatgtacttc tgaaccacca cgcactgtcg cgcgatgagc agggtctcca
      481 aaatggcctc ggctgttttg gccgtgtcgg ccaggatcac ccctaccccc tgagagccct
      541 cgatcagctt gatgatgatc ggggggcctc cgacctgcgc gatggcttct ttgacgctgc
      601 tacggtcacg cacgaaggcg gtcgccggga ttggcagctc gtgaccggca agaatctgca
      661 aggcgcgcag tttgtcccgc gacgccgcga tggactgcga cgagttgagc gcgaatacac
      721 ccttctgctc gaggtgcctg agcacggcac ttccgaaacc gctgatagag gctccgatcc
      781 gtggaatcac cgcgtcgtac tgggatacta gtcggccccg atagaacagc tcgaggcctc
      841 ggttctcgag caacagacta aagcccagcg tgttgaaaac ccgcggtacg tggccccgct
      901 tgacgcacgc ctgcttcaag cgctgcgtgc tgtaggcgcc agatgttctg gaaagaatca
      961 gtacttttag gggcatcgat gacacactgg agggctcggt ggcttggcag ggctgggcgt
     1021 ctgactagcg tcgaagtccc cgcgcgggga agtcgccgtg cggagaggtc aatgcgtagc
     1081 gctgcgcggg gtcaatcagg aagtagcggc ccaaagccgt gcgacccagt agcatgcggt
     1141 acagcatatt ggcgcggtcg acgaggccga gctcgatgtt ctggaccagc ggcccgatac
     1201 gcaaacccac actgacgaac acccttgccg aagcaacccc cgtactggcg cggactcgac
     1261 aacgtcgcgt gatgggcgct tcgatcgtca agcggcgttc cagtttgtaa cggtgcagcc
     1321 tcacttcgaa gcgcacgcgg tcacgcgaga tttcacacaa gttctctacg tgcagcgcgc
     1381 tgctccgagc gccggtgtcg actttcgcgc gtagttgaag cacgttccat tcaggaatat
     1441 cgacgtattc cgtaacgcca atgatcaggc ggtcgggatc tgccacagcg cggacggtag
     1501 cacttcgagc cagcgcggcg tcacgctttg gtccgggctc tcgacagatc agggccaggc
     1561 tgataaccgt ccctcgtggc ggacgagttt ccgacgctgg aaatcgtgtt ggcggcttgc
     1621 gtagcgggcc tcgcttggcg tgggttccgg ctattttggg cagagcggtc cctagccgct
     1681 caggtggaga agctgaaccc cgatctgccg aaccagggcc taactggggg gaacgtcgag
     1741 cgcgcgagcg cctacctggg cgtattgctg agagccgcag gggaggaaca agcacgcgct
     1801 gagtcccaag ctcagccgcg cgcttcggca cgaacagcgc caaccccttc ggagaagccg
     1861 agcgggccgc cgcgcgcgca cgtcgccgaa atagtccacg agcgcgcgtt acggatgcgg
     1921 cggcgacttc agggtgcctc agctcgagat ctgcttggct gtgccgtgct cggcggggcg
     1981 gccatgtatg ccggagccgc cgatctgggt gtcgcgaaaa cgctgttcgt gctgggtggc
     2041 ttgggcagcg ggctgttgct cttgtccgtg ctgctgcgca tgcgtctcga ttcgcgcgtc
     2101 gagctcgcag agaaggcggt cattgcctcg ttccgttccg gatcccgcgc ggcgcgctcc
     2161 ttttcgtcgc gtcctgccgc tgaccgttgt cccgcctgcg gacagccggg tatcgtacat
     2221 cacgagaccg ctgcggagct cggttcgcac ctgagcgcgt tgggcttgaa tgaactcgtg
     2281 cgctgtccca gctgtggtca catcgagggt cggattcggc cggtaaccgt cggctgacct
     2341 ggccaaaacc cgagtttcgc tctaatagcc aggtgcgcgg ccgaccaacc gccactgaca
     2401 atcatggatg catccggcag tcacgcttcc aaccgcagct actacgacgc cttttccgag
     2461 cgctacgaag cccagcgcgg ccccaacgac ccaggcggct atcacgacct cgtcgacgag
     2521 ctcgaggcgg agttcgtgcg gcgctacgga aatggcgcgg acgtactcga ggtcggctgc
     2581 ggcacggggc tcctgctcga acgcatccag cgcttcgcgc ggagcgtgaa aggggtcgac
     2641 ctgtcacccg gcatgttggc caaggcgcga gaacgcggcc acgacgtgct ggaggcctcc
     2701 gccaccgacc tgccgttcgc ggaccagagc ttcgacgtta cctgcagctt caaggttttg
     2761 gcccacgtca aagagattga acgcgccctg tcggagatgg cccgcgtgac gcgcgtgggc
     2821 ggctacgtca tcgccgagtt ttacaacccg tacagcatga gagcgttgct caagcgccta
     2881 gccccccccg gaaaagtcgc ggacggcacc cacgagcgcc atgtttatac gcgcttcgat
     2941 tcgccgcgag ccatcgcacg cctggtgccg agcaactgcc gctgggtcgc gagccgcggt
     3001 gtacgcatcg ccaccccgac cgcgcacgtc atgcggcatc gcgtgggcaa cgccgtcttc
     3061 cgtaagttgg aacggctgct gtgtgattcg ccgctgcggg cgctgggcgg attttacgtc
     3121 gtcgccctgg agagggtacg gtaagaaaag tgtctgcggt tccccagacc gaacgtcccg
     3181 cggcgtggtg gctcgcgact ctgaagcggc gggcgcagcg tcaatcggac gagtacgtgg
     3241 atctcagcca acccatcacg tggccgagcc ccgaagcaca gcgagcggcc ataaagttct
     3301 tcaacgccgc gttccgggct gaagaatctg ggctgcggca agcgcacgag ctggcgcacg
     3361 aagtggcgag ctgggacccc gagctcgcgg aagtgctgcg gctgtatggc aacgaggaag
     3421 gctggcaccg tgagctgctc accgagttcc tcgattacat cgggggccgc gtggagccca
     3481 tgggtcgcgt cacgcgaacg ctttactcgg tgtacgcgcg ggcgcaccgc atggagagca
     3541 tcgtgctgac gaacctgatg ttcgagtgca tcggttcgac gacgtatcgg ctgaccctcg
     3601 gccgcgtgga gcaccctgcg ctacgcaaga tgttgaccat cttgacgcgg gacgaagcat
     3661 ttcacgttcc tctcaacgtg ttctttctgc ggcaggtcct ggcccgcagc ggtcgcctcg
     3721 agaagcttcg cgtcgagtat ctatacaagc tgttgttctt ggcgctcgtg ctccttccaa
     3781 ttgcgagtcg tcgaaaagcg ctggccttcg acgggattcc cacgctcgag ttgatgcgtg
     3841 cttatgctcg cgcactcggc gggctcttcc cggaggggag cttccccaaa ggcagtcatc
     3901 tcagtatgcg tcccccgcgt ttggcgctct gggctttggg tttgcgcagc cgagatctac
     3961 aaggggatga gctgctccaa atcggaatgg atgccgcaga acgcgcagcg gatcgcgacc
     4021 aggtcgcggt tcgcggtcta gacttcaaaa acgccccgtg accccgagcg tgaaacggtc
     4081 gctctggaag ccgacgtcgg gaacgatcgt gaccggcgtg ggagccggcg gcacgtgcgg
     4141 tctacgcagc cgctcgggaa gcggatggac ccgctcctgc ttcacctccg ggacgaacgc
     4201 gaggtgggcc tggagcaccc caatcgccgc tactccgatc aacgcgtagc tactgacgtt
     4261 cagcgctgcg ttccatgtcc tcaggacctg gttgttctct tcgttgcgga gctgcgaggc
     4321 ctctcgcgcc aaatacgttt ggaacccgag cgacgtcaga gcggtggcgg acagcatgag
     4381 ctcacccgta aagaatgccc agccgagccc ctcatcgccg ttttggaatt gacccgctcc
     4441 aaacgggatc cacgcgatga atcggcggtt cttcacgacc agcacctcgc gtgcggcgag
     4501 acgctcgagc tccgccaccc gctccgcttc ggcgcgctcg cgcgccgcgc gggcctctgc
     4561 cgcttccctc gcctcctgca cgcgccgcgc ttcggcgcgc ttgatctcgt cgtacaaagt
     4621 ctgccgcacg cggaggaaac gctcgatgac cggcggcggg aagaccaggc tatcgggcgg
     4681 ccgcatctgc acgttggcgc ggatcgcggc cttcaatggc tcgtcggcct tctccggctg
     4741 acccgcccca atcaaacacg ccgcgtgata gatgcgggcg ctctcgacg
//
LOCUS       DASTKU010000213         5021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1155807, whole genome shotgun sequence.
ACCESSION   DASTKU010000213 DASTKU010000000
VERSION     DASTKU010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155807"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..1855)
                     /locus_tag="VFQ61_08080"
     CDS             complement(308..1855)
                     /locus_tag="VFQ61_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954447.1"
                     /translation="MSLIQAIGGEQRGILAPRALSAVFSLLALAHCSVNEPKRTYGAG
                     GSEGGTAGSGNGGLAGVPETSGAGTGAASNGGTKASTNSNEPLAGRGGSEFGSGGQSS
                     EEEATGGRSGGAPTVSGGGSGAVGASAQGGAPEGTAGTSGDSAGAAGESGTEAPLQVV
                     DLALGESHSCAVFSDQTVKCWGSNFLGQLGDGTDVGSRRPIRVASLAGVTSVAAKASH
                     TCAALTDGTVKCWGDNMNGQVVNGKIDTSYVPRLVPGLLNVVQVGTGPSHACALLEDQ
                     TVRCWGSNRWGQLGNGESGISPSGVEEKWVLQPTPVIGLAKATQVVAGNDFSCALLRD
                     GSARCWGNNYDRQLGNQSTEPSPTPVGVWTTANLTQLGAASIFVWGLAMDGKVTFWGA
                     KSPEARIIGSWDPEEVPGISDAIQVTGGGAHACALIRDGSILCWGTNWNGQQGIGTKT
                     RSDVVGKVLTVEPSVRVVAGDDHTCAIASDRRRVMCWGDNWAGQLGDGTITTAPSPVM
                     VEGLFDD"
     gene            2562..3668
                     /gene="dnaJ"
                     /locus_tag="VFQ61_08085"
     CDS             2562..3668
                     /gene="dnaJ"
                     /locus_tag="VFQ61_08085"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET9954448.1"
                     /translation="MSEKRDYYEVLGVAQSASADDIRKAYRQAALKYHPDRNGGDKKA
                     EALFKEATEAYSVLSDQEKRAMYDRYGHAGLGGAGVDFSGVGVNDILSHFQDMFADFF
                     GGFGGQGGAGQNRRRRAERGQDVRVSVELTLAESMRGGKHEVVVRGAAPCETCNGSGA
                     KAGTAPVQCTQCRGTGQVTAQRGFIMFSTPCARCRGTGMWIQDPCEACSGNGAVEKQR
                     RVLVTFPAGIDSGQRLRVPGQGMPGPERAPAGDLYVDVELVQDPRFERHGDDLATRAR
                     VSFATASLGGELTLTLPDDSTAQVKIAPGTQPGTVVLVRGHGMPRIDRSGRGDLHAVV
                     EINVPSKVSKRAKKLILELEDELNSSDSSEAHAP"
     gene            3832..4506
                     /locus_tag="VFQ61_08090"
     CDS             3832..4506
                     /locus_tag="VFQ61_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954449.1"
                     /translation="MSSDAKMSSELSGQEPVPEEPSKEASRPLSRAELDAQQAGDLLE
                     PSNKAEEVMLGARHDFSVKDSSGAIPCKCVGAMLGPPSSGKLQWRGDMPRTKPETQLV
                     LALVPDDSPSCGVTTAGAGGASYWGYRMEGDDVVVLLENWNSARPRTLGAIIPKPVEG
                     AQVYLAPVNPRFPYGTSTSDSGGRCALGNPGPKRSRSSGVEAGASPFPASSANRPVPT
                     EDESSQ"
BASE COUNT          921 a         1581 c         1623 g          896 t
ORIGIN      
        1 agtgaagtaa acccgggcag tcgcaagccc agctcagtag agacagctag agagcattcg
       61 aggcgcgcat gacctgcgcg cctcgaacgc gttaggcatc atccaggtga ctactagcgg
      121 gatgcgccgt gtcagtcttc acgagtaatg gcgctcggcg gctcgaggat aaggatcggc
      181 acgcaactgt gatccccgcg acgcgaagcc gcggcgaggc ctaccgcagc gtcctcgtca
      241 gcagctctgc ccgacgctca taaaggcgcg aggcgaccgc tttgaagcca gcggcgctcg
      301 attcagtcta gtcgtcgaac aggccctcca ccataaccgg cgagggagcc gtggttatag
      361 ttccgtctcc gagctggccg gcccagttat caccccagca catcactcta cgacggtcgc
      421 ttgcgatcgc gcacgtgtga tcatcaccgg caaccacgcg gaccgacggc tcgaccgtga
      481 ggacctttcc cactacatcc gagcgagtct tcgtcccgat accttgctgg ccgttccaat
      541 tggtacccca gcaaagaatt gagccatcac gaatcagcgc gcaagcgtgt gcgcccccac
      601 cggtgacttg aattgcgtca gagatgcccg ggacctcttc aggatcccaa gagccgatga
      661 tccgcgcctc cggcgacttg gctccccaga atgtgacttt gccatccatc gcgagacccc
      721 atacgaaaat gcttgctgca ccgagctggg tcaagtttgc ggtcgtccaa acaccgactg
      781 gcgttggcga cggctcggta gactgattcc caagctgacg gtcgtagttg tttccccagc
      841 aacgcgcact cccgtctcgg agcaacgcgc aactgaagtc gttgcctgcc acgacctggg
      901 tggctttggc caagccaatc accggagtcg gctgtaacac ccacttctcc tcaactccgc
      961 tgggactaat cccggattca ccgtttccca actggcccca tctattggat ccccaacatc
     1021 tgacggtttg gtcctccagc agcgcgcagg cgtgggacgg tcccgtaccc acctggacta
     1081 cgttcaacag tccgggaaca agtcgcggga catacgaagt gtctatcttg ccattcacca
     1141 cttggccatt catgttatcc ccccaacatt tcacagtccc gtccgtcaat gcagcgcaag
     1201 tgtgggacgc cttcgcggcg acgctggtta cacccgcgag tgacgcgact cgtatcggcc
     1261 gccgcgagcc tacgtccgtg ccgtcgccga gttgtcccag aaagttgctc ccccagcact
     1321 tgacggtttg atcactgaat accgcgcagc tgtggctctc gcccaacgcc aaatccacca
     1381 cctgaagtgg cgcctcagtt ccgctctcgc ccgccgcacc cgctgagtct cccgacgtgc
     1441 ccgcagtgcc ctccggagcg cccccttgtg ctgatgcacc aacggcccca ctccctccgc
     1501 cgctcacagt cggggctccg ccggatcgac cgcccgtagc ttcctcctcc gagctttgcc
     1561 cgccactgcc gaactccgat ccgccccgac ccgccaaagg ctcgttcgag tttgtcgaag
     1621 ccttggtgcc gccgttgcta gccgcacccg tgccggcccc gctggtttct gggacgcccg
     1681 ctaagccgcc gtttccggaa cccgccgtac cgccttcgga gccgccagcc ccataggttc
     1741 gcttgggctc gttgacgcta cagtgcgcta acgccaacag cgaaaacacc gccgaaaggg
     1801 cgcgcggcgc caagattccc ctctgctccc caccaatcgc ttgaattagg ctcatatcca
     1861 ggttcccaat acgcggcgaa ccatagcttc ttccctgcac agcgtcgacg gcaaggacgc
     1921 ctgcccccgg cgcgcgtgaa gacacgcaac gcacgagcat gacgctttca ccgcgttcgt
     1981 ggacctggcg ctgcaaagcc tcacgcctat ctaaaccacc ggagttatca cgcgcagcgt
     2041 gcgcggtcgc acgacgaaaa tcagccctgc gcagggctcg agtcagtgag gcccacgcag
     2101 cacgaccaat gtgccggcgt gcgaagagcg tctctttgcg ctccacgacc cgtcgatgaa
     2161 acccgggccg cactctcggc gaacacccgc acgactgagc aaagggacgc gccaattctg
     2221 agctgcgcct gaatacttgg tggtttgtcg tcctgtaacg atccccgtca cgttgcacac
     2281 cccggcgcgt tatccagatg agagtcgacg ggacgccgta accgcgagag cactcgtgag
     2341 cgttacggtg aggggcctgc gcggtggggc gggggctgag ggtgacctgg gctgcgctgg
     2401 tgagggctcg gactggcggc gcgcccgtgg tgctgtggcc gcctccccct tcccgagctt
     2461 ccgaagggaa agaatgcggg ttcttgaggg gggatgcgtt gacagggctc acgcccgaat
     2521 tagcttgggg ccaaccgttc cggtgtgagt cgagttccag tatgagcgaa aagcgcgatt
     2581 attatgaggt gctgggcgtg gcccagtcgg caagtgccga tgatatccgt aaggcttatc
     2641 ggcaggccgc cctgaagtac catcctgatc ggaacggtgg ggataagaag gcggaagcgc
     2701 tgttcaagga ggcgaccgag gcttactcgg tgctgtcgga ccaagaaaag cgcgcgatgt
     2761 acgaccgcta tggacacgcg gggctcggcg gcgcgggggt ggatttctcg ggcgtgggcg
     2821 tgaacgatat cctgagccac ttccaggata tgtttgcgga tttctttggg ggttttggag
     2881 gtcaaggcgg ggcgggacag aaccggcgcc ggcgcgccga gcgcgggcaa gatgtgcggg
     2941 tgtcagtgga gttgacgctc gccgagtcca tgcgcggcgg caaacacgag gtggtggtac
     3001 gcggcgcggc cccctgtgag acgtgcaacg gatcgggcgc gaaggctggt acggctccgg
     3061 tgcagtgcac gcagtgccga ggcacgggtc aggtgacggc gcagcgcggc ttcatcatgt
     3121 tttctacgcc ctgcgcgcgc tgtcgcggca cgggcatgtg gattcaagat ccttgcgagg
     3181 cttgttcggg caacggcgcc gtggaaaagc agcgacgcgt gctggtgacg ttccccgcgg
     3241 ggatcgactc ggggcagcgg ttgcgcgtgc cggggcaagg aatgcccggg ccggagcgag
     3301 cgcccgcagg tgacctttat gtggacgtgg agctcgttca agacccgcgc ttcgagcggc
     3361 acggagatga tttggcgacg cgcgctcggg tgtcgttcgc gacggcgtcg ctcgggggtg
     3421 agctgacgtt gacgttgccc gatgacagca cggcgcaagt gaagatcgca ccgggaacgc
     3481 aaccgggcac ggtggtgttg gtacgtgggc acggcatgcc gcgcatcgat cgctcgggcc
     3541 gcggtgactt gcacgcggtg gtggagatca atgtgccctc gaaggtcagc aagcgcgcca
     3601 agaagctgat cttggagctc gaagacgagc tgaattcgag cgattcgtcc gaagcacacg
     3661 ctccgtaaaa atggggcgct gagcgcgcgt ggcacaaacg cagcctcttt ttggggctgc
     3721 gtcgcccaaa gattgcctcg ggctcgccgg taatgcgacg ctagcagccg catgcttcgg
     3781 cttctcgtcc tagcaatggt tggctgctcg ctgggtggct gtcgcgcttc gatgtcatca
     3841 gatgcaaaga tgagctcgga gctgagcggg caagagcctg tgcccgaaga accttcgaag
     3901 gaggcctccc gcccgctgtc gcgcgccgag ctcgacgcgc agcaggcggg ggatttgctc
     3961 gagccgagta acaaggcgga agaggtgatg ctcggcgctc gccacgattt ctccgtgaag
     4021 gacagctcgg gggcgatccc gtgcaagtgc gtgggcgcaa tgttaggccc gccatcgtcg
     4081 gggaagctgc agtggcgcgg ggatatgccg cgtaccaagc ccgaaacgca gctggtgctg
     4141 gcgctggttc cggatgacag cccgtcctgc ggcgtgacca cggcgggcgc cggtggggcg
     4201 tcgtattggg gctatcgtat ggaaggcgac gacgtggtgg tgttgctcga gaactggaac
     4261 tcggcgcgac cacggacgct cggcgcgatt atcccaaagc ccgtcgaggg tgcccaagtg
     4321 tatctggctc cagttaatcc gcgattcccg tacgggacgt cgacttccga ctcgggaggt
     4381 cgctgtgcgc tggggaaccc gggtccgaaa cgcagccgga gctccggggt ggaagcagga
     4441 gcgagcccgt ttccggcctc ctccgccaat cgccccgttc ctaccgagga cgagtcgagc
     4501 cagtagctcg agggttggat ggcgaacagg cacggcggga gcgtcaggca ctcacgaggg
     4561 acagaggagc aggtcgcaag actcgcggct cgggccgaag agcctctccc gctcttgtct
     4621 aggtaggccc aagacccagc actggaaaat cgaaggcata tctgaaactc ggcacattgc
     4681 gggccgtatc agtcagtggc gcggccgaga agcgcaccac ccctcccccc cccaccccac
     4741 cagtgccctc ggtcgcgccg attaattcgt gggggaaacg ccgagagtat ttttcctctg
     4801 gcgaacgccg ctaggatccg cttcatcaaa caggtggaca tgcctgaatg cggagagaga
     4861 gcggtgctga tcattactgc gacgctcgag ggtacgtcga cgagtgtggc gcgcgagcgc
     4921 gtccctctgt gttgcagtcg cgagcgcggg cacgctgggc ctcacttgga ttcgagccgt
     4981 tcagaagagt gggaggcgga acccggtgtg ttgaagacgt t
//
LOCUS       DASTKU010000214         9667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_487697, whole genome shotgun sequence.
ACCESSION   DASTKU010000214 DASTKU010000000
VERSION     DASTKU010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487697"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VFQ61_08095"
     CDS             complement(<1..1085)
                     /locus_tag="VFQ61_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954450.1"
                     /translation="MQLALPFSLLTLLTIVACATESSPPLNSSGNTGNPPAQSGSPGT
                     GGKTNASSGGAVSGGASTTAGSASGGAATGGLGNGGRSGAGAMNGGSGSTQGGTGSTP
                     TSGGIGAAGASGGVASVSYALPPPSQCHNQDYIDYQEGCKEGDATSKCGGKCNTINAC
                     LESTSTKPHADTTFICPRFMLFSDAMRQAAMDDGNAAFNYAVVGHDVDRGGIDGTDNS
                     TCCQCYQLVYAYPSPANDRQAQKNPDNPSPPESAIAIPPPLIVQSFNTAATPHTFDVY
                     MAAGGLGANNACAPNLQPKAMSGQYLYSAYPDDGQPSQGGVKPASLFQECKTDRNWVT
                     AASLEAPACQSRIEAACSEIKSDVPGLT"
     gene            1381..2256
                     /locus_tag="VFQ61_08100"
     CDS             1381..2256
                     /locus_tag="VFQ61_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954451.1"
                     /translation="MSGTLGMLSAVLLGVGCLTLCRIGHAGDLFEIQVYEAEVNNPLQ
                     AGLEAHLNYTFSGRRSAEYAGEAVPHHAARLTLEPALGLTEYLEVGAYLQNMIDRDGT
                     YRFAGWKLRTKWVVPRRYTGRFFLGINAEIGKVPRAVEEHGWANEFRPILGYDDGHWL
                     FDVNPIFGYALSGPDKLRPDFEPAGKVAFNTQLGFSIGTEYYAGLGYLSALPAPRSEQ
                     DHLLFLTFDLAQPASAKSLRAPRTEAQSTSGENPTPESDEAELPFELNVGLGRGLSDA
                     TPQRWIVKAIFGRAF"
     gene            2383..4254
                     /locus_tag="VFQ61_08105"
     CDS             2383..4254
                     /locus_tag="VFQ61_08105"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9954452.1"
                     /translation="MKTVPSISLLAITGFSICLACSSSTPEGRRGTSGRSPSGAGGNA
                     PLLPTSQNIPKAPQIGVDLSGLPATPQCGDGVLTQDEACDDGNRNPGDGCLANCRQVE
                     LGYSCVPAGQACHPIARCGDGVLVFPELCDDGNRAAGDGCSASCKVEVGFKCSGGPST
                     CTPTTCGDGKIEGAESCEDGNARPFDGCSADCQNEPKCVNGPCSSSCGDGIVLGEACD
                     DGNNLDGDGCSAQCQPEPGFVCTQPDVGETMQVPIVLRDFKFHNPTDFEPSATGQNAA
                     VVGLVDGTLDAEGKPVFVGTANPYGAPFIAGPDSFAKWYRDVPGTNHTTISKLTLYKN
                     AAGAYVNRMKNTGEQFLKTATAYYCGNVGAELTDEAGNPIPCTSKFGATDCDKVTGPI
                     LSCTINNTSYVATYLLEAIDGNPLFYPVDGDDFTPDAERTSAVLPAPMYSSDWGPEPG
                     KPLHNFSFTSEAGYWFKFDASKTYVLDFTGDDDVWVFINRRLAVDLGGIHTPVNGSVT
                     LDMSTAGKFGLEDGKVYEIRVFQAERQTTSSSYKLTLSGFSAAPSDCHPVCGDALIGI
                     GEECDDGVNDGGYGECGPGCRLGEFCGDGIQQAGEDCDDGINVGNPCPSGCRKITIY"
     gene            4484..5905
                     /locus_tag="VFQ61_08110"
     CDS             4484..5905
                     /locus_tag="VFQ61_08110"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET9954453.1"
                     /translation="MDSLKDWPQIIQGGMGIGVSDYRLARATSKLGCLGVVSGTGLDT
                     VLLRRLQLGDPTGEMRRALAHFPWPDLVERVLARYFVPGGKPERQPFRLLPMPNANMS
                     RERLELIVLANFAEVWLAKEDGGRIGINFLEKIQTPLLASVFGAVLAGVDAVLIGAGI
                     PFGIPEILDHMCRWEASEMRLALEEAPSQGSYAMRFDPQELFGIPPLLIQRPKFFAII
                     SSHVLAKVLHKKTHGGVDGFIVEDHRAGGHNAPPRKTPGRAPQSPPAFGPADLADLEE
                     LRAVGVPFWVAGCRATHASLQEALEAGARGVQLGTVFAFCDESAVAPELKRDVLSHVQ
                     EGRAHVVTDFDASPTGYPFKRIEREGSPNELEALRGRERVCDLGYLRRTFVDGKGHVA
                     YRCPGEPVDTFVSKGGDPAETQNKLCLCNQLFATIGLGQPRETGDELPLLTSGEDLPN
                     LRALLPPGKSTYTARDVVDYIMA"
     gene            6040..6627
                     /locus_tag="VFQ61_08115"
     CDS             6040..6627
                     /locus_tag="VFQ61_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HET9954454.1"
                     /translation="MISFGSLLALASGLAMDAAAVSAARGFATPLLRVRHALLVAVFF
                     GGFQGLMPLLGWLLGRQLGPIVASWDHWIAFVLLGGIGGKMLWEAREAKAEPTDAEKP
                     NSAEALYALPVLLALAVATSIDAFAVGIMLPMLGADLILSSLTIGVTTACLSILALIA
                     GRHFGAMFGKKLDALGGLVLIALGTKVLIEHLSAG"
     gene            complement(6681..7181)
                     /locus_tag="VFQ61_08120"
     CDS             complement(6681..7181)
                     /locus_tag="VFQ61_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor synthesis domain-containing
                     protein"
                     /protein_id="HET9954455.1"
                     /translation="MSGLSIHVITVSDTRTPATDSSGRMLFDRLSEGGHRVSGPEIIP
                     DDLAGIRSTLQRCVAAPEIDVVVLTGGTGITRRDVTPEALAPLVTKSIPGFGELFRML
                     SYAEIGASTIQSRAAAALCDGTLVFALPGSTNAVRLALEKIILPQLDAKTKPCNFVEL
                     LPRIRA"
     gene            7367..8287
                     /locus_tag="VFQ61_08125"
     CDS             7367..8287
                     /locus_tag="VFQ61_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954456.1"
                     /translation="MRQLAIWTLALGLAVPGLACSNIDPLPAPTNAKPAEETPSPVLE
                     SGGAGATNARMGQAGRSRSESNGGQANLATSATTGGSSPATSSMASPSPDPAQQVSGT
                     GGTTSAPRLPVGEASDAPGGSGGAAGESSRGAAGESSRGRDCEAWFSEYVEGSGNNKA
                     LEIAASAQCELTGCSVLIFANGSETATRHVELTGTVAPSSPLVLCTAQLADKIARCDE
                     IEALSFNGNDAVLLSCGESVLDRIGRVGEDPGEAWGDARVSTQDMTLQRDCRSRGDSM
                     ARAAFDVASTWIAKPLDTFEALGADCTSAR"
     gene            8584..>9667
                     /locus_tag="VFQ61_08130"
     CDS             8584..>9667
                     /locus_tag="VFQ61_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9954457.1"
                     /translation="MTVDNLPPRPPTAFRSVGILCNSFGDPYTVPILHGAAKELSAQG
                     YHTICLTGGFPNAPIYQGIDQQPAIPPFLDGLILLSATMRGFGSELEQMASGSPQAVV
                     SIGASLGQLPSIAANDETGIFQAVAHLVKRHDRKLIAFIGGPEGSTDGSRRQAAYRIA
                     LENFNLPCDPSLVVRGDYEARSGREAVRALRQMSSKSFDAIVAANDLMAIGAMEGLQA
                     AGIRVPGKVSVIGFDDMEEAAFTVPSLTTVRQPVYEQGISAAHLVLRLLKGGTIEDQV
                     TIIPTPLVLRHSCGCATSDSGQRMAAVVATDMLESYEKLEDAMRSRIRRHLAAQRQHR
                     ELSRLADRLVSVVDFPELASVMSEVFR"
BASE COUNT         1762 a         2984 c         3098 g         1823 t
ORIGIN      
        1 gtaagccctg ggacgtcgga tttgatttcg gagcaggccg cttcgatgcg gctttggcaa
       61 gccggcgcct ccaaagatgc cgcagtgacc cagttgcggt cggtcttgca ctcttggaat
      121 aggctggctg gtttgacgcc accttgacta ggttgaccat cgtccggata agcggagtac
      181 aggtactgac cggacatggc ctttggttgg aggttgggtg cacacgcgtt gttggcgccg
      241 agacctcccg cggccatgta gacgtcgaac gtgtggggag tcgcggccgt gttgaagctc
      301 tgcacgatca gaggaggcgg gatcgcgatc gcagactccg gcggactcgg gttgtccggg
      361 ttcttttggg cctgacgatc attcgccggg ctcgggtacg cgtacacgag ctggtagcat
      421 tggcagcacg tgctgttgtc cgtgccatcg atgccaccgc gatcgacgtc gtgcccgacc
      481 acggcgtagt tgaacgcggc attcccatca tccattgcgg cctggcgcat ggcgtcgctg
      541 aacagcatga agcgcgggca gatgaaggtc gtgtctgcat gcggcttggt gctggtgctc
      601 tcgaggcagg cgttgatggt gttgcacttg ccaccgcact tgctcgtggc gtcaccttct
      661 ttgcagcctt cttggtaatc gatgtagtcc tggttgtggc actgacttgg gggcggcaat
      721 gcgtagctga cggacgcgac gcctccgctc gctccggccg cgccaatccc accgctcgtc
      781 ggtgtgctgc ccgtgccgcc ttgggtcgag ccgctaccgc cgttcatcgc tcctgcgccg
      841 ctgcgcccac cgttgcccag tccaccagtt gcggctccgc ccgacgcgct ccccgcggtg
      901 gtagaggcgc ccccgctcac ggcaccgccg ctgctggcgt tcgtcttccc accggtgccg
      961 ggagagccag actgcgccgg cggattaccg gtgttcccac tcgagttgag cggcggcgac
     1021 gactcggttg cgcaggccac gatcgtcaag agggtaagca aagagaacgg aagggccaat
     1081 tgcatgtgcg ttacggaaac gggtagcaga attccggcgc tgcctcgtgc tgaccgtggt
     1141 ctgcgtttga tggatctcaa gtcagctcga cccgagatga cgtcgctcaa cgggccaggc
     1201 gccagcgggt ccgcgcagtc gcggcaaagc gcgcaagcct tgcgggtgat tcgaatcaat
     1261 cgacgagcac ggcgcaaaga cttccaacag gtgagctctt ttagcaattg aaattcaaat
     1321 tcaggtagag catgtcagtg tcgcatgagc cgcctccgct ccgccgttcg ctattcttcg
     1381 atgtctggga ccctcggtat gctcagcgca gtgctgctgg gtgtcggttg tttgacgctg
     1441 tgccgaatcg ggcacgccgg ggatctgttc gagatccaag tgtatgaagc cgaggtaaac
     1501 aatccgctgc aggcgggcct ggaagcacac ctcaattata cgtttagtgg ccgacgcagc
     1561 gccgagtacg cgggtgaagc cgtgccgcat cacgccgcac gtctcactct ggagcccgca
     1621 cttggcttga ccgagtatct agaagtcggt gcctacctac aaaacatgat tgatcgagac
     1681 ggcacttatc ggttcgcggg ctggaagctg cggaccaagt gggtagttcc gcgacgctac
     1741 acgggtcggt tctttctcgg catcaatgcc gagatcggca aggtgccgcg cgcggtcgag
     1801 gagcacggct gggccaatga gtttcgaccc attctgggct acgacgatgg ccactggctg
     1861 ttcgatgtga atccgatctt cggatatgcc ctatctggtc cggataagct gcgtcccgac
     1921 ttcgagcctg ctggtaaagt agccttcaat acgcagctcg gcttctccat tggcaccgag
     1981 tactacgccg gcctgggtta cctgtcagcg ctgccggcgc cgcgttccga acaagaccac
     2041 ttactcttct tgacgttcga tctcgcgcag ccggcgagcg cgaaatcttt gcgcgctcca
     2101 cgaacggagg cgcaatctac gtcgggagaa aatcccacgc ccgagagtga cgaggccgaa
     2161 ttgccattcg aactcaacgt gggactaggc cgcggacttt cggacgcgac gccgcaacga
     2221 tggatcgtca aagccatctt cggcagggcg ttctaagagc ccgctacgct caagcacacc
     2281 gcgtgcctcg tcggacatca cgcttgacgt cgatcaactc acctgcgcga tcgaagtcgg
     2341 tgcggcaccc aaggcctggg aatccgctac tgtgtgtggg gcatgaaaac ggttccatcg
     2401 atttcacttc ttgccattac cggtttcagc atctgcttag cttgctcgag ctcgacccca
     2461 gagggtcgac gaggcacctc cggccgctcg ccgagcggcg cagggggcaa tgctccgcta
     2521 ctgccgacat cgcaaaacat ccccaaggcg ccgcagattg gcgtcgatct gagtgggtta
     2581 cccgcgacgc cccagtgcgg cgacggcgtg ctcacgcaag acgaggcttg tgacgacggc
     2641 aatcgcaacc ccggtgacgg atgcctcgcc aattgccgcc aagtcgagct cggttattct
     2701 tgcgttcctg ccggccaagc gtgccacccc atcgcgcgct gcggtgacgg ggtgctggtg
     2761 ttcccagagc tttgcgacga tggtaaccgt gccgcaggcg acggttgctc ggcgtcttgc
     2821 aaggtcgagg tgggcttcaa gtgcagcggc ggcccgagca cttgcacgcc aaccacttgt
     2881 ggcgacggga agatcgaagg cgccgagagc tgtgaagacg gcaacgcgcg tccgttcgat
     2941 ggctgttcgg cagactgcca aaacgagccc aaatgcgtga acgggccttg ctcgtcgagt
     3001 tgtggtgacg gcatcgtgct cggtgaggcc tgcgacgacg gcaacaacct ggatggcgac
     3061 ggctgctccg cccagtgtca gcccgagccc ggtttcgtgt gcacgcaacc cgatgttggc
     3121 gagaccatgc aggtcccgat cgtgctgcgc gacttcaagt tccacaaccc caccgatttc
     3181 gagcccagcg ccaccggtca gaatgccgcg gtagtaggcc tggtagacgg cacgctggac
     3241 gcggaaggca agcccgtatt cgtcggcacc gccaacccgt atggggcacc gttcatcgct
     3301 ggcccggaca gtttcgccaa gtggtatcgc gacgtgccgg gcaccaacca cacgacgatc
     3361 tcgaaactaa ccctctacaa gaacgcggcc ggcgcctacg tcaatcggat gaagaacacc
     3421 ggcgagcagt tcctgaagac tgcgaccgca tactattgtg gcaatgtcgg ggccgagctc
     3481 accgacgaag ccggcaaccc catcccttgt acgtcgaagt tcggcgccac cgactgcgac
     3541 aaggtaacgg gcccgatcct gagctgcacg atcaacaaca cgagctacgt cgcgacttac
     3601 ctgctcgagg ccatcgacgg taacccgctg ttctatcccg tggatgggga tgacttcacg
     3661 ccggacgcgg agcgcacgtc ggcagtactg ccggcgccga tgtactcctc ggattggggc
     3721 ccggagccag gcaaaccgct gcacaacttc agcttcacga gtgaggccgg gtattggttc
     3781 aagttcgacg ccagcaagac ttacgtgctc gatttcacgg gcgacgacga cgtgtgggtg
     3841 ttcatcaatc ggcgcttggc cgtcgacctc ggtggcattc atacgccggt caatggctct
     3901 gtcacgctgg acatgagcac cgctggtaag ttcggtctgg aagacggcaa ggtatacgag
     3961 attcgcgtgt tccaagcgga acgccaaact acctcttcta gctacaagtt gaccctgagc
     4021 gggttcagcg cggcgccgag tgactgccat ccggtctgcg gagacgcgtt gatcggcatt
     4081 ggcgaagagt gcgacgacgg cgtcaacgac ggcggttacg gtgaatgcgg gcctggctgc
     4141 cgcctcggag agttctgcgg tgacggcatt caacaagccg gggaagactg cgacgatggc
     4201 atcaacgtgg gtaatccctg cccgtccggt tgccgcaaaa tcacgatcta ctgagctccc
     4261 ttcggtctga atccgtgagc ggtatggccg cacacgcggc ctaccaaggc ttggccctcc
     4321 ccggcatcga ctcgggaggg ccaagttgta tgcgccgttc cctcactccg aatcagtggt
     4381 ctcgatcgcg gccccagcgc tgtatgtcat gcgtccgtca gcatgcggtc atatcgatgc
     4441 tgacgaagtg ctcgtactct tccgcttgag gtaagaggat tcgatggact cgctaaagga
     4501 ctggccgcaa atcattcagg gcggcatggg tatcggggtt tcggactatc gattggcgcg
     4561 cgccacgtcc aaactcggct gtttgggcgt cgtctcgggg acgggtctgg acacggtcct
     4621 gctccggcgc ctacagctcg gcgatccgac tggggagatg cggcgagccc tcgctcactt
     4681 cccctggccc gacttggtgg agcgtgtcct cgctcggtac ttcgtgccgg gtggcaagcc
     4741 cgagcgtcag ccatttcggc tgcttccgat gccgaatgcc aacatgagtc gcgagcggct
     4801 cgagctgatc gtactcgcca acttcgccga ggtgtggctc gccaaggagg acggcggtcg
     4861 catcggcatc aacttcctgg agaagatcca aaccccgctc ctggcttcgg tgttcggggc
     4921 ggtgttggcg ggcgtggatg cggtgttgat tggcgccggc atcccgtttg gaatcccgga
     4981 gatactggat cacatgtgcc gctgggaagc ctcggagatg cgcctcgcat tggaggaagc
     5041 cccgagccag ggatcgtacg cgatgcgctt cgacccccag gagttgttcg ggatcccacc
     5101 gctcctgatt caacgcccca agttcttcgc catcatctcc tctcatgttc tcgccaaggt
     5161 tctgcacaag aagacgcacg gcggagtgga tggcttcatc gtcgaagatc atcgagcggg
     5221 aggccacaac gcgcccccgc gcaaaacccc gggtcgtgcg cctcagtctc cgcccgcttt
     5281 cggccccgct gatttggccg atctcgagga gctacgcgcg gtgggcgtgc cattctgggt
     5341 tgcgggttgc cgcgcgactc acgcttcctt gcaggaagcg ctggaggcgg gcgcgcgggg
     5401 cgtgcagctc ggcacggtgt tcgcgttttg cgatgaatcc gcggtggcac cggagttgaa
     5461 gcgtgacgtg ctctcgcacg tgcaagaggg gcgcgcccac gtcgtcaccg atttcgatgc
     5521 gtcccccacc ggatatccgt tcaagagaat cgaacgagaa ggctccccca acgagctcga
     5581 ggcgctacgc ggacgcgagc gcgtgtgtga tctcggctac ctacgccgta ctttcgtgga
     5641 cggcaaaggg cacgtcgcct accgctgccc aggggaaccc gtggacacct tcgtgtccaa
     5701 aggtggagac ccagcggaga cgcaaaacaa gctctgccta tgcaaccagt tgtttgcgac
     5761 gatcggcttg gggcagccgc gtgagaccgg tgacgagtta ccgctgctca cgtccggcga
     5821 agacttgccc aatcttcgcg cgctactccc accgggcaag tcgacctata cggcgcgtga
     5881 cgtggtcgat tacatcatgg cgtgaggttc ggcgttccga atgcagagca ccgaacgcag
     5941 agcagcgagc tcggcgcgtt cgataagcgc ggcggagtgg ggccgcggcg atcgaggtgt
     6001 ggccttgcgt gaacacgagg atctgtcagg gtcggcgacg tgatttcgtt cgggtcgctg
     6061 cttgctttgg cttcgggtct cgcgatggac gcggccgcag tgtcagcggc ccgcggcttt
     6121 gccacgccac tccttcgcgt gcggcacgcc ctgctggtgg ccgttttctt cggcggcttt
     6181 cagggcttga tgccgctact aggatggttg ctcgggcgcc agctcgggcc gatcgtggcg
     6241 agctgggatc attggattgc attcgtcttg ctaggcggaa ttggcggcaa gatgctgtgg
     6301 gaagcgcgtg aagcgaaggc cgagcccacc gatgccgaaa agccgaacag cgccgaagcc
     6361 ttgtatgcgc tgcccgtcct cttggcgttg gccgttgcga cgagcatcga tgccttcgcg
     6421 gtgggcatca tgctgccgat gctcggagcc gatctcatcc tctcgtcgct cacgattggc
     6481 gtcacgaccg cgtgcctgag catcctggcc ttgatcgccg ggcgtcactt cggagcgatg
     6541 ttcgggaaaa aactggacgc cttgggagga ctggttctga ttgcgctcgg caccaaggtt
     6601 ctgatcgaac acttgagtgc gggctgaacg aaaggcccgc cgtgtttacg aggccgtgtc
     6661 ggcaatgttt accagtccat tcaagccctg atacgcggga gcaactcgac gaagttgcag
     6721 ggcttcgtct tggcgtcgag ctgcggcagg atgatcttct cgagcgccag gcgtaccgca
     6781 ttggtcgagc ccggtaacgc aaagaccagc gtaccgtcac agagagcggc ggcggcacgg
     6841 gactgaatcg tcgaagcgcc gatctccgcg tagctcagca tccgaaacaa ctctccgaag
     6901 cctggaatgc tcttggtcac cagcggagcc aaagcctccg gcgtcacgtc ccttcgggtg
     6961 atccccgtac cgcccgtcaa gaccacgacg tcgatctccg gggcggccac gcagcgctgc
     7021 agggtggagc ggatgcccgc tagatcatcg ggaatgattt ctggtccaga tacccggtgc
     7081 cccccttcac tcagccgatc gaacagcatg cgaccgctgc tgtcagtagc gggagttcgg
     7141 gtgtcgctga ccgtgatgac gtgaattgaa agtccgctca cgggcgcgac cttagcactg
     7201 accccaaagc actgcgacca ctcccctccc cggcgagcgc aacttttcgc gaacctttcc
     7261 gcaactggga agcgcgagcg gccgaatcaa ggacatgtca tgcgaccgct tctctcaatc
     7321 tccctggccg ccgctgtcgt cgccactgtc cgcagtggtg ctcgccatgc ggcaacttgc
     7381 aatctggacg ctcgcgctgg gtcttgccgt tcccggtctg gcctgctcga acatcgaccc
     7441 cctgcccgcc ccaacgaacg ccaagcctgc tgaggagacg ccgtccccag tgctggagag
     7501 cggtggcgcc ggcgctacga acgcacgcat gggtcaagcc ggcaggagtc gctcggaatc
     7561 gaatggcggc caagccaatc tcgccacgag cgcgaccacc ggcggttcgt caccggcaac
     7621 gtcgtcgatg gccagtccct cgcccgaccc tgcacagcaa gtgagtggca ccggaggcac
     7681 cacgagtgca ccgaggctac ccgtcggcga ggccagcgac gcgccgggcg ggagcggcgg
     7741 cgccgcgggc gaatcgagcc gcggcgccgc gggcgaatcg agccgtgggc gagactgcga
     7801 ggcgtggttc agcgagtacg tggaaggctc cggcaacaac aaggcgttgg agatcgccgc
     7861 ctctgcgcag tgcgagctca cgggctgcag tgtgctcatt ttcgcgaacg gctccgagac
     7921 cgcgactcgt cacgtggaac tcaccggaac ggttgctcct agctcaccgc tggtcctgtg
     7981 caccgcacaa ctggcggata aaatagcgcg gtgcgacgag atcgaagcgc tatcgttcaa
     8041 tggcaacgac gcggtgttgt tgagttgcgg cgaaagcgtt ctcgaccgta tcggccgagt
     8101 gggggaagat ccaggggaag cttggggcga tgctcgggtg agcacgcagg acatgactct
     8161 gcagagagac tgccgcagcc gaggtgactc gatggcccga gccgcgttcg acgtcgcctc
     8221 gacctggatc gccaaacccc tggatacatt cgaggctctg ggtgcagact gcaccagcgc
     8281 acgctgaaag gtcacggctc acatgcgcat gggcgggggc ggcgacaccg gcgtctggct
     8341 cggcccatgc gacccgcgga gtgcctcggg cgcctcgcaa aaacggatcg aggcaacaca
     8401 tctcggatgt catttagaag ctatcccggt aattgggata gcttcctagg ggtgagatag
     8461 gcagacaatt ccggagacgc gagagagcac gcgcgagagt gcgtgtcgga cttcgactta
     8521 cagcaaatcg taagaaagaa tagggactta acctcactcg taaaagccgc tagcttctga
     8581 cgagtgacgg tagacaacct gcctcctcgc ccacccaccg cgtttcgctc cgtcgggatc
     8641 ctctgcaact cgttcgggga tccatacacg gtgcccatct tgcacggcgc ggcgaaggag
     8701 ctctctgcgc agggctacca tacgatctgc ctgaccggag ggtttccaaa cgctccgatt
     8761 taccaaggga tcgaccagca gcccgccatt ccaccctttt tggacgggct gatcctgctg
     8821 agcgccacga tgcgcgggtt tggcagcgag ctcgagcaga tggcgagcgg cagtccccaa
     8881 gcagtggtga gcatcggcgc gtcactcgga caactaccga gtatcgccgc caacgacgaa
     8941 accgggatct ttcaagcggt agcgcacctg gtgaagcgtc atgatcgcaa gctcatcgcc
     9001 ttcatcgggg ggcccgaggg cagcaccgac gggtcacgcc ggcaagccgc atatcgaatc
     9061 gccctcgaga acttcaatct gccatgtgat ccttctctgg tcgtgcgcgg cgactacgag
     9121 gcacgctcgg gtcgggaggc ggtgcgtgcc ctgcgccaga tgagcagtaa gagcttcgac
     9181 gcgatcgtgg ccgccaacga cttgatggcg attggggcca tggagggact gcaggcggcg
     9241 ggcattcgcg tgcccggcaa ggtcagcgtg atcggcttcg atgacatgga agaggccgcc
     9301 tttacggtgc cttccctgac gacggttcgc cagcccgtgt acgagcaagg catctcggcc
     9361 gcacacctgg tattgcggct gctcaagggc ggcacgatcg aagatcaggt cacgatcatt
     9421 cctacgccgc tggtcctcag acattcctgc ggttgcgcga cctccgactc ggggcagcgc
     9481 atggcagcag tggtcgccac ggatatgctc gagagctacg agaagctgga agatgcaatg
     9541 cggagccgga tccgcagaca cttggcggcg cagcgccagc atcgcgagct atcgcggctc
     9601 gccgatcgcc tggtcagcgt ggtcgacttc ccagagctcg cgtcggtcat gtccgaggtc
     9661 ttccgct
//
LOCUS       DASTKU010000215         3706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1379591, whole genome shotgun sequence.
ACCESSION   DASTKU010000215 DASTKU010000000
VERSION     DASTKU010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379591"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(328..1119)
                     /locus_tag="VFQ61_08135"
     CDS             complement(328..1119)
                     /locus_tag="VFQ61_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9954458.1"
                     /translation="MTRKTAEPRDPSDPCERLRCLGLYGLIARWPEIATQPWIEQLLQ
                     IEEAERKQRSLDRRLRNAKIGAFKPIDLFDWAWPTKIDRQAIEELFSLTLLDDGANAI
                     LLGPNGVGKSMILKNLAQHALVRGHTVRFTTASDMLAELAAQDSATALARRLRRWALP
                     RLLCIDEVGYLSYSSRYADLLFEVVTRRYDAGRSIVVTTNKSFNDWSEVFPHAACTVT
                     LVDRLVHRSEIIEITGESYRLKEAKERSAAKAKQRKHKPSHTHPA"
     gene            complement(1100..2602)
                     /gene="istA"
                     /locus_tag="VFQ61_08140"
     CDS             complement(1100..2602)
                     /gene="istA"
                     /locus_tag="VFQ61_08140"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HET9954459.1"
                     /translation="MIDKAVEANILRLFHAEKWPIGTIAAQLHVHHTTVQRVLAQAGL
                     APEQVSPRSSMSDPYTPFIVETLEKYPSLCASRLFQMVRERGYPGGPDHFRRVVARFR
                     PRPKHEAFLRLRTLIGEQAQTDWGAFGKLLIGRALRSLWAFVMVLSWSRQIFLRFYLS
                     AAMPSFVRGHVDAFAFFQGVPRIILYDNLKSAVLERVAHAIHFNPRILELARHYHFEP
                     LPVAQARGNEKGRVERAIRYVRTSFFPAREFKDIDDLNRQAFEWTTGVAAERRSHEDH
                     AKQVRDAFAEEQPKLLPLPQEPFPSDEVVSVETGKTPYARFDLNDYSVPHTHVRRSLT
                     VAASLDTVRVLDGTHVIATHARSWDRAQQIEDPTHIKPILELKRRAREHRGLDRLSSA
                     VPSAQALLVQAAEQGGNIGNITARLLLLLDQVGASELEQAVASAVERKLPTVGAVRQI
                     LDKRFAERGVPPPVSSRLSTNERATRVTVKSHSLSTYDHLRRTEDDQENS"
     gene            complement(2599..2958)
                     /locus_tag="VFQ61_08145"
     CDS             complement(2599..2958)
                     /locus_tag="VFQ61_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954460.1"
                     /translation="MFKPWRCTLHVTSTAATLLVTDPTHDLLKARLDPCPSHPRALLT
                     LLEGLSLWGGERLRVALSVEEGCLRGLGSTLLGDELWPAESALVRYDLVHPVRPKRLD
                     GIGDFRYLRRVARRCGQ"
BASE COUNT          691 a         1120 c         1184 g          711 t
ORIGIN      
        1 gggcgcgcag ccagcccagt caatcccgcc gaatcaagtc gtatccgctt cacgaaggcg
       61 tgcgaccggc cgaatcaaag tccgcccaat cgtattcgtt tcagggggga aagtccgccg
      121 aaccaagtcc gccgaatcaa gtcgttcacg atggcaacca gcccaagccc ctccagccac
      181 gggcgcgcag ccagcccagt caatcccgcc gaatcaagtc gtatccgctt gacgaaggcg
      241 tgtcgacgag cgcgagaaac cgcgggattc cggtgaagag gcttggggtg accaacgccc
      301 ggaatcccgc gaggtgtggg cggctgctta ggcgggatga gtatggctag gcttgtgttt
      361 gcgctgcttg gccttggccg cgctgcgttc cttggcttct ttcaggcggt agctctcgcc
      421 ggttatctcg atgatctcgg agcggtgtac gaggcggtcg acgagggtga cggtgcaagc
      481 tgcatggggg aagacctcgc tccagtcgtt gaaggatttg ttggttgtga cgacgatcga
      541 tcttccggcg tcgtagcggc gagtcacgac ctcgaacaac agatccgcgt agcgtgagct
      601 gtacgataga tacccaactt cgtcgatgca gagcaggcga ggcaacgccc agcgacgcaa
      661 acggcgagcg agggccgtgg cgctgtcttg agctgccagc tcggcgagca tgtcactggc
      721 tgtcgtgaat cgcacggtgt ggccgcggac gagggcgtgt tgggcaagat tcttcagaat
      781 catactcttg cccacgccat tgggcccgag taggatagcg ttggcgccgt catccaggag
      841 tgtgaggctg aagagctcct caatggcttg tcggtctatc tttgtcggcc atgcccaatc
      901 gaagagatca atcggtttga aggcgccaat cttggcgtta cgcaacctac ggtcgaggct
      961 acgttgtttg cgttctgctt cctcgatctg cagcagctgc tcgatccaag gctgggttgc
     1021 aatctcgggc cagcgagcga tgaggccgta caggccgaga caccgaaggc gctcgcacgg
     1081 gtcactcggg tcacgtggct cagctgtttt cctggtcatc ttcggtcctt cttaggtggt
     1141 cataggtcga aagggagtga ctcttgacgg tgacgcgagt ggcccgctcg ttggtggaga
     1201 ggcgggacga aaccggtggt ggaactccgc gctccgcaaa gcgcttatcg aggatctggc
     1261 gtacggcacc gacggttggc agtttgcgct cgacggcgct agctacggct tgctcgagtt
     1321 cgctcgcacc gacttgatcg aggagtagaa gtagacgagc ggtgatgttg ccgatattgc
     1381 cgccttgctc ggcggcttgc accagaagtg cctgtgcact cgggaccgcg ctactcaacc
     1441 gatcgagccc tcggtgctca cgcgcacggc gttttagctc tagaattggt ttaatgtgag
     1501 tagggtcctc gatctgctgg gcacgatccc aagatcgcgc gtgagtagcg atgacgtgag
     1561 tcccgtcgag gacccgaacg gtgtccaggg aggccgcgac ggtcaacgag cggcgaacgt
     1621 gtgtgtgcgg aacggagtag tcattgagat cgaagcgggc atagggtgtc ttgccggtct
     1681 cgacgctcac gacttcgtcc gaggggaacg gctcttgagg tagagggagc aatttcggct
     1741 gctcctccgc gaaggcgtcg cggacctgtt tggcgtgatc ttcgtgagat cgtcgctcag
     1801 cagccacgcc tgtcgtccat tcgaaagctt gtctgttgag gtcatcgatg tctttaaact
     1861 ctctcgctgg gaagaagctg gtgcggacat agcgaatcgc gcgctcgaca cgtcctttct
     1921 cgttgccacg tgcttgggcg acggggagcg gctcgaagtg gtagtggcgt gcgagctcga
     1981 ggatgcgcgg gttgaagtgg atggcatggg cgacccgctc gagcaccgca cttttcagat
     2041 tgtcatacaa gatgattctg ggaacaccct ggaagaacgc gaaggcgtcc acgtggcccc
     2101 ggacgaagct gggcatggcc gcgctcaagt agaagcgcag aaagatctgg cgtgaccagc
     2161 ttaggaccat cacgaaggcc cagagcgagc gcagcgcacg gcctatcaag agcttcccga
     2221 acgcgcccca gtcggtctgg gcctgctcac cgatcagggt acgcaatcgc aagaaggctt
     2281 cgtgcttggg ccgtggtcgg aagcgggcga cgacgcgccg aaagtggtcc gggccacctg
     2341 gatacccgcg ctcacggacc atctggaata gccggcttgc gcacagacta ggatacttct
     2401 ctagagtctc gacgatgaac ggggtgtacg ggtcggacat cgacgagcgc ggactcacct
     2461 gctcgggagc gagcccggct tgggccaaca cgcgctgcac ggtggtgtgg tgcacgtgaa
     2521 gctgcgcagc gatggtacca atcggccact tctccgcgtg aaacagccgc aggatgttgg
     2581 cctcgacggc cttgtcgatc attgtccaca ccgccgagca actcgccgca gataccggaa
     2641 gtcaccgatt ccgtcgagcc gcttggggcg aacggggtga acgagatcgt accggactag
     2701 cgcgctctcg gccggccaca gctcgtcgcc gagcagggtc gagccgagcc cacgcaggca
     2761 gccctcctcc acagaaagag caacgcgcag cctctctccg ccccacaggc ttagtccctc
     2821 caggagcgtc agcaacgctc gcggatgact tgggcacggg tcgaggcgcg ccttcagtag
     2881 gtcgtgtgtt ggatccgtca ccagcagagt tgctgctgtc gaggtcacgt gcagtgtgca
     2941 tctccaaggc ttgaacatcg ctaggagcct ccatcgctcc gggctggctc gcgtcttctg
     3001 cgagaccaga tgtccgaggg atgccgagcc ctggctcttg cgtcgagcac tggtgagtca
     3061 ctatctgtgc aggaaagttg gcccatctca gcttgtcacg cagtcgctgt tgccggtcag
     3121 cgtgcagccg cattccgcga cgcgtctgct ggaaagcctt ggagtgaact cgccattgcc
     3181 gtcggcgccc agcgactaca cactcgtcag agcagtactt ttgtccccgc tcacatcgcg
     3241 gacagaccct acagactcga tggcaccccg gacagtgcag gaaccgccaa aactctcttt
     3301 gaccgtctgc gcacactgcc ctcccgaatc agatccgatt tctggacgcc ccgagcgaac
     3361 cctgcgatac gcccctatgg cgcgtcgcag actgcgcgat cgccaggaag gctcgggcgg
     3421 caacccgggc cttcaaacct tttctcggga gccatcagcc tatgagccga cgctcctccc
     3481 ggccaagttt acgctgcttc ccgcgcaccg cccccctggt gaagcaatct ggcggcctcc
     3541 cgcgctcttc accgacctga cgcggaaact ggcgctcgcc gacaggtgtg gagcagcatg
     3601 cccgacgacc aactcttcga tacggctcgc agcggcgagt tgctgtcaga cgaggcccta
     3661 gcggccgagc tccatcgcct gctcgccagc ccgcgctccg ctcagt
//
LOCUS       DASTKU010000216         7097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_487846, whole genome shotgun sequence.
ACCESSION   DASTKU010000216 DASTKU010000000
VERSION     DASTKU010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487846"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            824..2563
                     /locus_tag="VFQ61_08150"
     CDS             824..2563
                     /locus_tag="VFQ61_08150"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9954461.1"
                     /translation="MGGTGASSGSSGSSAKGGAGGASVDSGGSIGGAGASSGSSGSSA
                     KGGAGGAPSGGSGGGTGKGGSGGTTSTVPDKPGPKTNFTEQVGSVPFDMVYVPGGTFT
                     LGCESGSCPQYTDPVADVKVSSYYIGKTEVTTALWNAVMGTSSSGIGGTGGSMTSITW
                     YNAMEFACKLGQMTGRKYRMPTEAEWEYAAKKQLSSLEKVGTGEEWAYNSWETAYSCK
                     SGAVDPVGPGSGQHTQKTRRDAQGTADNITGRLIRSIDGIGPALRLAISDETAFPPTY
                     VSPCDLHAPAMGSEPVNSYRDPRWITGSDAHWTTGSIAIGNFDLRVWEDGTARLGTTN
                     GQWFTSNNIAFVFVPSSGAIKKFPYIFLDDTQGSLLSETSFMSGGYIGRIAKEAATSY
                     AKPTVSGLKSGAELAAAAGDDFKMVDMVNIPEAAKKQDPRLLDGPTQGWFQDNRSAGG
                     VHHYRKDVDPDEFRFTVNQGTSRTMLANGKWFTVNNTFLRITHSSGYTTDYLYAVDAD
                     GTFYHDSFQAYERADFRMFKKTANGSETFAATCGSICSDEIPKGEKASLYSTLDNGKS
                     TFVPAPCPPEGCK"
     gene            complement(2600..3148)
                     /locus_tag="VFQ61_08155"
     CDS             complement(2600..3148)
                     /locus_tag="VFQ61_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9954462.1"
                     /translation="MADVKDKLLDVAYRVLEKEGASALTTRRVCEEAGVTMPTLYHHF
                     GNRDGLLSAVHGVTLEKFMAKKRALAPTADPIADLRGGCEMVLEFAADHPAVTLAVLA
                     RAVEQPSMLSQGYALMKHRIERCGKSDLLRVPVGQAAEHVWAIVQGLSLSIVSNPTGV
                     PPSRKTLRAALDALFEALFGRV"
     gene            3408..4433
                     /locus_tag="VFQ61_08160"
     CDS             3408..4433
                     /locus_tag="VFQ61_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9954463.1"
                     /translation="MAELVFITGGSSFVGAAIIQAMISEGHRVRALARSEKSARTVSL
                     LGAEPVRGDLDNERSLAKGMLGATIVVHAAASLTSGIRYGDHERTNVEGTRKVLESAR
                     QAMVRRFIYISAASVVIQAGRATHGDESLPVVYDRSMPYSATKGLAERIVLGANDGSM
                     STVALRPPFIWSENAPSIRHIAHAFHEGRFMWIGGGEYNYSVCHVDNLASAVVRSVHH
                     GQGGKAYFVTDDDVTSMRQFFTHLIEATGQPAKARVVPYWFGALLARMIGLVFALLRP
                     GQQPPLTLEAVRLMGKRLELSNALAKRELGYVPEVSRAVGISRIRAAASNESVGHGAA
                     HASVSAQ"
     gene            4430..5098
                     /locus_tag="VFQ61_08165"
     CDS             4430..5098
                     /locus_tag="VFQ61_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954464.1"
                     /translation="MTPANGLTIATSPRKVEAVLRMLGVRRGARAVPVAIVVTLAMIS
                     TLSPRGACAAEAPREQPPPVRVLPASEILWPLLQLIPSPEVAFGDGRTTAALRWELTP
                     LLYSFGLREPSSRVRAFFVPPPARFTGSFELVGSVEYAPGPPGVSDWVARLGARVTLP
                     LVDRGEALALSLGTAAFLNDGDVRPSFDVGLLTLFGTCGIRATLSPELAGRQIIVTLV
                     LRFF"
     gene            5227..6033
                     /locus_tag="VFQ61_08170"
     CDS             5227..6033
                     /locus_tag="VFQ61_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9954465.1"
                     /translation="MRSLPETSTSGISVLWVGHATVLIRLGERYVLTDPVFSRTVGML
                     SPRLVQPGLPVHEVPNITATLVSHTHFDHLSFSSLDALGTRTGTLLVPRGILEYLPRF
                     PFPERGIGPWSSIVLDGVTITAVPVAHPGGRYGLDESTCGDCSTGFVLEHDGWTIYFG
                     GDTAYASARFQETRQRFPHIDLAILPICPIAPRDFMRKTHLSPGEALRAHRDLGARWM
                     MPIHYDTFINSDDAPGDCLKELHASMRRMGLPSSEVIELKVGERRVIPQH"
     gene            complement(6039..6686)
                     /locus_tag="VFQ61_08175"
     CDS             complement(6039..6686)
                     /locus_tag="VFQ61_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954466.1"
                     /translation="MRLALLSIGAPFALLLSRLALAQSAPPSQIQLEEPKPRQGHFVA
                     AGVYGIGAMGFDASRGTREPTLGQGFTLRLGESLTSWLSLSLAAGYGFTYGPQRDKLS
                     IIRFGISTQWYITERWSLQAGFGATSAQGPDPQDYGRNRSRYGAFGLVGAGYDVYLTN
                     GAQSGGWIVTPTITADVGPTSKLTTTSLWLGIELSYWTGVSRDKLRLSTPNAYSK"
     gene            complement(6689..>7097)
                     /locus_tag="VFQ61_08180"
     CDS             complement(6689..>7097)
                     /locus_tag="VFQ61_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954467.1"
                     /translation="GKSNYRLFDHDQTHILTVTGSYRLPKNWEIGTRFRYVTGKLYTP
                     VRAAVYDSDSDTYSAINAPTNSARVDAFHQLDLRIDKRWIYESWMLNAYLDIQNVYSR
                     SNVSGIEYSYDYSKKVPESGLPIVPVFGLRGEF"
BASE COUNT         1363 a         2224 c         2189 g         1321 t
ORIGIN      
        1 ccaagaacga cctgaacgcg gcacaggtcc ccgacgctgc cgcagcaatc tgaaggtgct
       61 caagccacta aacatgcact ggcctcgatc tacaaaatat cttctcacag ctcaataacc
      121 tgccgaacgc gtgatcttaa aacccagtca cggaatcgga ctgagagtgg tgttctcgcc
      181 gctcggtgca agcacgcagt cccgcggctg gcattccgct actacgctca attccctcca
      241 ctgttccgag tgacggaggt gaggacagca tgaccgcggt cagtcgagac tctcccattt
      301 cgccctaaac caatacaaaa cgattcatcc aattcggaga cgcgaatggt gaattggccg
      361 gctgatcgcg gtctggggca gcgcgaggga cgttacccga cacaatttct gacccagttg
      421 accgtcgtca agcgcatgca tccgctcgac gggaatacag cattgaggcc acacagatgg
      481 gctactcaat atcgagaagc aacccactgc aggtgctagt cgcgtcgcgc gcggaggaaa
      541 catgtgtatt tggaaactaa agcagtcttc gtggggtgac ctcgccggtg cccttggtag
      601 cgtccgcgcg cggcagcggc actgtccaga gctgctttca ccgcgcaggc ggcccggagg
      661 caccatgaac cgggtacgaa cgtcgccctc ctgtctctgc ctcgcgctct tcgttgcggc
      721 atgcggctcg ggacagggaa actcgccatc ccagagtccg ggcccgggcg gagcccagaa
      781 tggaggctcg ggtggagcac agggcggagt gtccggaagc tccatgggtg gtactggcgc
      841 ttcgagcggt tcgagtggca gcagcgccaa aggcggcgca ggaggagcca gtgtagactc
      901 cggcggctcg attggcggcg cgggtgcttc gagtggatcg agtggcagca gcgccaaagg
      961 cggcgcagga ggagccccga gcggaggctc cggcggcggt accggcaagg gcggctcggg
     1021 cggcaccacg agcaccgtgc cggataagcc cggtcccaaa accaacttta cggagcaagt
     1081 aggcagtgtg ccattcgaca tggtttacgt gccgggcggc acgttcacgc tcgggtgtga
     1141 aagcggcagt tgcccccaat acactgaccc ggtcgcagac gtgaaagtca gcagctacta
     1201 tatcggaaag accgaggtga cgacggctct gtggaacgcg gtgatgggca cgagttcgtc
     1261 cggcatcggc ggcacaggtg gctcgatgac gagcattacc tggtacaacg ccatggaatt
     1321 cgcctgcaag ttggggcaga tgacgggcag gaaataccgt atgccgaccg aggccgagtg
     1381 ggagtacgcg gccaaaaaac agctgagtag tttggaaaag gtcgggaccg gcgaagaatg
     1441 ggcctacaac tcctgggaaa cggcatattc gtgcaagtca ggcgccgtcg atcccgtggg
     1501 gccaggcagc ggacagcaca cgcagaagac gcggcgagat gcgcagggaa cggccgacaa
     1561 tataacgggg cgcctcatcc gatccatcga cggaataggc cccgctttgc gcttggccat
     1621 ctcggatgag acggctttcc ccccgactta tgtctccccc tgtgatcttc atgcgccggc
     1681 gatgggcagc gaacccgtga actcctatcg cgatccacgc tggatcaccg gcagcgatgc
     1741 acattggacg accggttcca ttgcaatagg caatttcgac cttagggtct gggaggacgg
     1801 cacggcccga ctgggtacca ccaatggcca gtggttcacg tcgaacaaca tcgcgttcgt
     1861 tttcgtcccg agttcaggtg caatcaagaa gttcccctat atctttctcg atgacaccca
     1921 aggctcgctc ctgtccgaga ccagcttcat gagcggtggt tacatcggga ggattgccaa
     1981 ggaggcggcg actagctatg ccaagccaac tgtctcgggt ctgaagagcg gcgcggaact
     2041 cgcggcggca gcgggggacg acttcaagat ggtggatatg gtcaacatcc ccgaagccgc
     2101 aaaaaaacag gacccgcgat tgctcgatgg ccccacccag ggctggttcc aggacaaccg
     2161 cagtgcgggc ggcgtgcacc actacagaaa ggacgtagac ccagacgaat tccgtttcac
     2221 cgtcaaccaa ggaacgagcc gaaccatgct cgccaatggt aagtggttta cggtcaacaa
     2281 caccttccta cgtatcaccc actcgagtgg ctatacgacc gactatctgt acgcggtaga
     2341 cgcggacgga accttctatc acgactcgtt ccaagcctat gagcgcgccg acttcaggat
     2401 gttcaagaag acggccaatg gcagcgaaac cttcgctgcc acctgcggca gcatttgtag
     2461 tgacgaaatc ccgaagggtg agaaagcttc gctctattcc accctcgaca acggcaaatc
     2521 caccttcgtg ccagccccgt gccccccgga aggctgcaag taggtaattc aacgcgccgc
     2581 ggccggcata ccggcattcc tatactctcc cgaacagcgc ttcgaagagg gcgtcgagtg
     2641 ccgcccgcaa agtcttgcgg cttggcggaa cgcccgtcgg gttcgagacg atcgagagtg
     2701 agagcccctg aacgatcgcc cagacgtgct cggcggcctg tccgaccggc acacgcagca
     2761 aatcgctttt cccgcagcgt tcgatgcggt gcttcatcaa cgcgtaccct tgcgagagca
     2821 tcgagggttg ctcgaccgcg cgcgccagga cggccagcgt aaccgctgga tgatccgcgg
     2881 cgaattcgag caccatttcg cagccgccgc gaagatcggc aatgggatcg gccgtcggcg
     2941 cgagcgcccg cttctttgcc atgaactttt cgagcgtcac gccgtgcacc gccgacagga
     3001 gcccgtcgcg gttgccgaaa tgatggtaga gggtcggcat cgtcacgccc gcctcctcgc
     3061 atacccgtcg ggtcgtgagc gctgatgctc cttctttctc gaggacgcgg tacgcgacat
     3121 ccagcagttt gtccttcaca tcggccatcc ctggaaactt agcatagcta ccctgcgacc
     3181 accgcgcgct ggcctcatcg gggagcccag ggtgcccggc tacccctcgc gcccattcgc
     3241 aggctctcga cccgtcgcgt gccggctcga gtctggtctc taaaggacgc ttcgcgagcg
     3301 gttgctcgcc acgtttttag aattcgtata gcggggctat agacgccgcc tgtagcggcg
     3361 ctatagtaat cctcgccggc ccatcgctgg cgggaaggca gaacagaatg gccgagttag
     3421 ttttcatcac cggaggcagc agcttcgtcg gcgccgccat cattcaggcc atgatttccg
     3481 aagggcaccg cgtacgcgca ctagcgcgaa gcgagaaaag cgctcgaacg gtttcgttgc
     3541 tgggagccga gcccgtccga ggcgatctgg ataacgagcg gtcgctcgcg aaaggtatgc
     3601 tgggggcgac gatcgtcgtg cacgcagccg cgtcgttaac gagcgggata cgctacggcg
     3661 atcacgagcg caccaacgtg gaaggcacgc gtaaggttct ggagagcgcg cgccaggcca
     3721 tggttcggcg tttcatctac atcagcgcgg catccgtcgt catccaagcc gggcgcgcca
     3781 cgcacgggga cgagtcgctg ccggtcgtct acgatcggtc catgccgtat agcgcgacca
     3841 agggcctggc cgagcgcatc gtgctcggag cgaacgatgg ctcgatgtcg accgtcgcgc
     3901 ttcggcctcc cttcatttgg agcgagaacg cgccgtcgat tcgccacatt gctcacgctt
     3961 tccacgaagg gcgcttcatg tggattggtg gtggcgagta taactacagc gtctgccacg
     4021 tggacaatct cgcgagcgcc gtggttcgca gcgtgcatca cgggcagggc gggaaggcct
     4081 acttcgtaac ggacgatgac gtgacgagca tgcgccagtt cttcacccac ctaatcgagg
     4141 cgacgggcca acctgccaaa gctcgcgtcg ttccgtactg gttcggcgcg ctactggcgc
     4201 ggatgatagg gctcgtcttc gcgctgctca gacccggcca gcagccgcct ctcactctcg
     4261 aagccgtacg actgatgggc aagcgcctcg agctgtccaa cgcgctcgcg aagcgcgagc
     4321 tgggttatgt gcccgaggtc tcgcgcgcag tcggtatttc gcgcattcgc gcagccgcat
     4381 cgaacgagag cgtcggacac ggagcggcgc acgcttcggt ttccgcgcaa tgacgccggc
     4441 aaacggcttg acgatcgcga cgagccctcg caaggtcgag gcggtgctca gaatgcttgg
     4501 tgttcggcgc ggggcccgcg cagttccagt cgcaatcgta gtgacgctcg cgatgatctc
     4561 gacactgagt cctcgagggg cttgcgccgc cgaggcacca cgggaacagc cgccccccgt
     4621 tcgcgttctc cccgcttccg agattctgtg gccgcttctg cagttgatcc cgagtcccga
     4681 agtcgcattc ggcgacgggc gaaccaccgc agcacttcgg tgggagctca ctccattgct
     4741 ctattcgttc gggcttcgtg agccgtcgag tcgtgttcgc gcgttcttcg tcccgccgcc
     4801 cgctcggttc accggctctt tcgaactcgt gggatcggtc gagtatgcgc ccggcccacc
     4861 tggagtcagc gactgggtcg ctcgacttgg cgcccgcgtc acgctacccc tcgtcgaccg
     4921 aggcgaggcg ttggctttgt ctctgggcac ggcagcattt ctgaacgacg gcgacgtgcg
     4981 cccctcattc gacgtcggcc tcctaacctt gttcggaacc tgtgggatcc gcgcgacgct
     5041 ttcgccggaa ttggccggac gccaaataat cgtgacgcta gtgctgaggt tcttttgaac
     5101 ggcgtcgagg tccttttgaa atcgctgcgc gtgctgccag cgctcttcct gcttggttgc
     5161 ggatttccgt ttacctcgat ggcacgaaac ctcgcgaggt tgacgagcag cccgtcccca
     5221 gcgcccgtgc ggtcgctccc cgaaacgtcg acgtcgggca tttcggtgct gtgggtcggc
     5281 cacgcaaccg tcctcattcg gctgggggag cggtacgtac tcaccgatcc agttttctcg
     5341 cgcaccgtcg gcatgctctc tccgcgcctc gtccaacctg gcctccccgt gcacgaagta
     5401 ccgaacatca ccgcgaccct cgtttcgcac acccacttcg accacctatc gttttcatcg
     5461 ctcgacgcgc tgggcacgcg gacgggtacc cttctagtgc ctcgtgggat cctggagtac
     5521 ctgcccaggt ttcccttccc ggagcgtggg attggcccct ggtcgtcgat cgtcctcgac
     5581 ggagtgacga ttacagcagt acccgtcgcg cacccgggcg gtcgctatgg tctcgacgag
     5641 tccacatgcg gcgactgctc gacgggattc gtgctcgaac acgatggctg gaccatctac
     5701 ttcggaggcg atacggccta cgcctcggct cgattccagg agacccggca gcgatttcct
     5761 cacatcgatc tcgccatcct ccccatctgc cccatcgctc ctcgcgactt catgcgcaaa
     5821 acccacctgt ctccaggcga agccctccgc gcccaccgcg acctgggcgc cagatggatg
     5881 atgccaattc actatgacac tttcatcaac tccgatgacg ccccgggcga ctgcttgaaa
     5941 gaacttcacg cgagcatgcg gcgcatgggg ctcccctcga gcgaagtcat cgagctgaaa
     6001 gtgggagaac ggcgcgtcat tccacagcac tgaagcccct acttggagta tgcgttgggc
     6061 gtcgatagtc ggagcttgtc gcgcgacacc ccggtccaat agctgagctc gattccgagc
     6121 cagagcgagg tagtcgtgag cttcgaggtg gggccgacat ctgcggtaat tgttggggtg
     6181 acgatccacc cgccactttg cgcgccattc gtcaggtaga cgtcgtaacc ggcgcccacg
     6241 aggccgaagg cgccgtagcg gctgcgattg cgaccgtagt cttgcgggtc gggcccctgc
     6301 gcgctggtcg cgccaaaccc ggcctgaagg gaccagcgct cagtgatgta ccattgagtg
     6361 ctgatgccga accgaatgat cgagagcttg tcacgctgag gaccgtacgt gaagccgtag
     6421 cccgccgcga ggctgaggct gagccagctc gtgagtgact caccgagccg gagcgtgaaa
     6481 ccctgtccta acgtcggttc gcgtgtccca cggctcgcgt cgaagcccat cgcgccgatg
     6541 ccatagaccc ctgcggccac gaagtgacct tggcgaggtt tcggctcttc gagctggatc
     6601 tgagacggcg gcgcgctttg ggccaaagcg agccgggaca agagcagggc gaagggcgcc
     6661 ccgatcgata acaaggcgag gcgcatgact agaactcccc ccgcaggcca aagacgggca
     6721 cgatcggtag gcctgattca ggcacctttt tcgagtaatc gtagctgtat tcgatacccg
     6781 aaacgttgct ccgactgtag acgttctgga tatccaagta cgcgttcagc atccacgact
     6841 cgtagatcca gcgcttgtcg atgcgcaggt ccagctggtg aaaggcgtcc acgcgcgccg
     6901 agttggtcgg cgcgttgatg gcggagtagg tgtcagaatc cgagtcgtaa accgcggctc
     6961 gcactggagt gtaaagcttg cccgtcacgt agcgaaagcg cgtgccaatc tcccagtttt
     7021 tgggcaaccg gtagcttccg gtgacggtga ggatatgggt ctgatcgtgg tcgaagaggc
     7081 ggtagttgct cttaccc
//
LOCUS       DASTKU010000217        14495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_709842, whole genome shotgun sequence.
ACCESSION   DASTKU010000217 DASTKU010000000
VERSION     DASTKU010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14495
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709842"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VFQ61_08185"
     CDS             complement(<1..980)
                     /locus_tag="VFQ61_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9954468.1"
                     /translation="MAEFTWEARARTGELRKGVMEADNEEAVNQRLRQQQLNPVKVAR
                     RRATSFQLGSGVGTKDLVTFTRLFATMIDAGLPLVQCLEILSGQQSNRHFSGVLKDIQ
                     GAVEGGSTFSEALRRHPKVFDELFVNLAHAGEVGGILDSILNRLSVYLEKRQKLVRQV
                     RGAMVYPSIVIVIASGVMTVLLTFVIPAFENMFKDFGGGKEALPKLTQIVVDVSHLFV
                     TALPLLMFGGLAFAVGFGYLLRTPRGKRGFHQLLLRLPIMGDVLRKIAVARFTRTLGT
                     LLQSGVPILDALEICAKTSGNVVIESGIMSVRQAISEGRNMAEPLTQTGI"
     gene            1507..2781
                     /locus_tag="VFQ61_08190"
     CDS             1507..2781
                     /locus_tag="VFQ61_08190"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9954469.1"
                     /translation="MGAFPTAGSPASNSGLTSTAPHPLGSRRGNHSSIEACLTREVIE
                     AGVARAASAGIAVRAGQGWRCASAHVGSLRPASFGAIDERAVYDLASVTKPFVALAAA
                     RLVEGGELTWDTPLCRMLPESSDTPVAAASLELLLSHRAGLEAHRELFGPLCARRPMD
                     RRAALRAALHARRTDCSGPVPVLGFPPVYSDLGYILVGEALAQHQQSGLERLVQREVC
                     KPLQLTVGSARTLLSAHPPQTEEHAAEPGMPRNLVGRASPFRERVVPTEVVAFRGGVV
                     RAVVHDENAWALAGHGMAGHAGLFGTLDDVLCLGRRLLDVLAGRDMTWLTSPTLEAML
                     ADRPGGTLRLGFDGKSTTGSSAGNRASSRSFGHLGFTGTSIWCDPEADAVSVLLTNRV
                     CPSRSNTKIRACRPVVHEALFELAQSLRAGLH"
     gene            2929..4371
                     /locus_tag="VFQ61_08195"
     CDS             2929..4371
                     /locus_tag="VFQ61_08195"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9954470.1"
                     /translation="MWKLTIEDDQSNRTAVHLVRTSYGLGRGEDNAIRLTERNVSRRH
                     ARLNQEGQRWIVEDLSSYNGCFVNGQRVSGTQTLSHGDLIQLGDYRLVLEDESLSTGT
                     QDITATVPAGPRAPSGTAAEDRLVMLMGPTPNAQFALSKNSMLIGRGEDCDISINHPS
                     VSRVHAELHPVADGRYEIVDKGSANAVRVNGAELPRSLLDARDVVELGDVVLKFIPAG
                     ELYLPGLDDSQIVGLSGITEKPGPPDAPEKRSKPFWSRWGWFGGSALLLLGVVVVVRG
                     RSDGPRDLVSIKDEATARAERILVEAKALMARGDLRAAQRKLSELPVDAPSRASSDFR
                     TIQAAYADYLFTLADQTSEPREKRNLYNEIARSPSIDSARRSRAEEQLAQLSEPALNV
                     ADLPSARERPPAPPAASGENVAGGSALAARFAEPGQADAPAREATPPAATGSVNRPAV
                     AVRPTTSAARPSGQRKSPNGVPTVIRDNPY"
     gene            4426..5904
                     /locus_tag="VFQ61_08200"
     CDS             4426..5904
                     /locus_tag="VFQ61_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954471.1"
                     /translation="MQPVEKPWSVLASRPALSAYGVLALALALELVPGAQRLRVLSRP
                     PEPDSSVARSALPEATATRVGEAELKTESEGSAALAPLALAPAAQQAARAEPEPKVPI
                     VDASGKALHKFYRALQRTQSKQTNATTRIAHFGDSIVASDYVSGTLRRRFQEAFGDAG
                     HGFSLIANAWPAYFHSDVERYATAGWKVSRVVGPLAEDGLYGLGCVSFKAEKNALARF
                     GTTSKGDFGRAVSRFEIFYLEEPSGGAFQISVDGQVREVVDTHGPSKASRTRVIQVPD
                     GPHELEILTKQGTSRLFGVVMERDAAGVVLDAIGIQGARIRFLDKQEDAHYAEQLKLR
                     NFDLLVYEFGANESADGFLYPMPEYHDTMKQVIAQGQAALPESSCLVIGAMDRAVQKG
                     DELIGIRVLPFLIEEQRKVAAELGCGFFDTYTAMGGRGSMAKWVKRGLGQADLTHPTA
                     VGSDVIGTWIYRALWRGYQDFLSTQKPSAPSSSADSRSPSSR"
     gene            complement(5847..6764)
                     /locus_tag="VFQ61_08205"
     CDS             complement(5847..6764)
                     /locus_tag="VFQ61_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9954472.1"
                     /translation="MPPARRSKHGDGLFGYYGLRVTPKGWPAMLSLQASPSAISGRAR
                     FDVLLESRGRSALTLKLGAQTVLVRDGQVHDGQIQKAASDRGAGAPPLHEAPACSRAP
                     GSSIEHVELEGQGTDTLTLEIQTGRPRLHGVIVESEQPGVVLDTVGIDGARIATALAW
                     DEESFIAELAARRPDLIVLAFGTNEAFDALRVSAYDQELSSLLGRARRAAPDSECLVL
                     GPPDALDVEGGSVARIPEIAGVYAQTAARLGCAFYSLQAAMGGPGSFAAWQRTEPRFA
                     QADRIHLTKDGYRELGERLSAELLGALGF"
     gene            complement(7291..8406)
                     /locus_tag="VFQ61_08210"
     CDS             complement(7291..8406)
                     /locus_tag="VFQ61_08210"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9954473.1"
                     /translation="MTELADAVLVIGFPGQRAVHITRELLDGGAGAVARLTLLVHPER
                     LQEAQTQIASWSEAARERVTLEPADPIAIDWGLSGSRYRQLAECTRAIHAAYSSLDED
                     LPEATAEALHIGQARELVEFSKVASRLRTLVLYSSVFVSGDRTGRVLEDELVAGQGFR
                     NPLERTLALTERMLADAGIQPWVLRSGHLIADSRTGAFARPTLPHLLLALAVNSREGA
                     RLPALPGSKARLAVTPVDYLARFGAWVHRLAEPGHTVHVFEPHGLSVGELWEHALERL
                     GKLPQEANSNLAWARALLNNPAARLVSEHPRSFWSALTPAAEYDQTRYAELLARGAPP
                     CPSLFSYLDPLLEQLRIRTQDERLSSEPEVDPPLAVT"
     gene            complement(8455..10086)
                     /locus_tag="VFQ61_08215"
     CDS             complement(8455..10086)
                     /locus_tag="VFQ61_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954474.1"
                     /translation="MPETPVERPATVLFELEVPEPQRVYAAIRELWAPELPRSAELAL
                     GHVLGFGPLVATSFDVEAPFVAAYAQNDEATSTGDWVFAARVRSGAELAAQLTTGSSP
                     SHHVEIVRGQPCLVASSSAASATGTSKPQAASPSIGTSKPRWPLGVTVFGNVAVFGGC
                     ELNSSIGPYAAQGVAQRVARAGAVRGRVTRGGLRSWMNELLVERWSALRAQLESAARS
                     DELHRGRPADFADPAGALAKLNRVVETVREFVGEASGLVFDVERDGEFVKWHAFMEGQ
                     SPLWSKLKDRTVAVPSLIWSLPSDTAFAVSLQGLWQEPAAGKSAEQPGPALGIWGHRA
                     TPQQIAQLERAWNDVAGALTGEQTLGLRSGRIWVGTGQVQDASLLERGLKGLFSGFSR
                     PPLEGPLSALLGKPTLIPARKSPGGTQFRASFATTDRAQTQQLVVEVGSDRYWFASAA
                     VPAGGSPAADGPALSREPWVSNWMRQPQEAIATLYLNPAALGGSARPAPGFVEFGLER
                     GSPALWGSFSAALIELARRAQLLPGAFPSNAGKGP"
     gene            complement(10223..10699)
                     /gene="ssb"
                     /locus_tag="VFQ61_08220"
     CDS             complement(10223..10699)
                     /gene="ssb"
                     /locus_tag="VFQ61_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET9954475.1"
                     /translation="MADGLNRVMLLGNLGADPELRMTQGGQAVLKLRLATTESYLDRN
                     RVRQEKTEWHSVVLWGKRAEALNKILSKGSRVFVEGGLRTSSYDDRDGNKRYRTEVVA
                     TNLILAGGARGGGGGGGGGRGPASDDYGGPGEEMGPGGGGGGDDSDYGGGDDDIPF"
     gene            complement(10795..11541)
                     /locus_tag="VFQ61_08225"
     CDS             complement(10795..11541)
                     /locus_tag="VFQ61_08225"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9954476.1"
                     /translation="MAGDTELPVKLYYRIGEVAQIVGVEPHVLRYWEGEFRSIRPQKS
                     RKGQRVYSRRDVEKLLKVKELLYTHGFTIAGARRRLREGGPDLSPGSEPSEGSAQPAQ
                     LAADSPALDHARDAQELTRERSAVVDAGHDEVAPFALSQLLAGNMAGSRVANASPAPA
                     ARAVIQLEGAPENRIGAEGTQAHSGIEAAQPGVAPAQPGVEASRDPRRIPVERDGRPD
                     ALPLHARAELCALREQVRAMLAELSFSSRR"
     gene            complement(11565..11894)
                     /locus_tag="VFQ61_08230"
     CDS             complement(11565..11894)
                     /locus_tag="VFQ61_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HET9954477.1"
                     /translation="MTKADIVQAIYTRLGGFSKKEAADLVDLVFETMKETLGRGEKIK
                     ISGFGNFVLRDKRQRQGRNPQTGDPIVITERRVLNFKASQLLKQALNDDVPSAEAAAA
                     VSPEASR"
     gene            complement(12070..13599)
                     /locus_tag="VFQ61_08235"
     CDS             complement(12070..13599)
                     /locus_tag="VFQ61_08235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9954478.1"
                     /translation="MYPVLVILYCSVLLVLAAYGLHRAHLAYTCMRHARRIDRLAKRI
                     DVSEEALPFVTVQLPLFNEATVARRLIESTGAMDYPRDRFEIQVLDDSTDETVALAEA
                     AVRDLAARGIQAHYVRRPNRHGYKAGALDHGLKSARGELVAIFDADFIPQPSFLREVV
                     GHFQDPQIGMVQTRWDHLNRSASLLTSIQALMLDGHHLVENRARFVNGCMFNFSGTGG
                     IWRREAIDRAGGWQHDTLTEDLDLSYRAQLIGYRFVYRADVRTPAELPEDMSAFRTQQ
                     YRWAKGTVQTARKLLGRVWAAPLPVHQRVEAAFHMLPHFAYPMTVLLTLVLLPALVFM
                     PATNIQTLIAVDLPLCIGATGSLASFYSMAEVAQGRSAWEAIRRLPMLIALGAGLAPH
                     LSSAVWDGLRFNAGEFVRTPKRGSAQGRYRQYNKLPLAEMFLAAVSAASVFAALTTGH
                     WLAAPFAALFCAGYSYVAVLVVNEQVLGARSAPQLAPAGVPLEAGADASRESGEMVRA
                     A"
BASE COUNT         2720 a         4774 c         4515 g         2486 t
ORIGIN      
        1 aagatgccgg tttgggtcaa cggctcggcc atgttgcggc cttcgctgat ggcttgccgc
       61 accgacatga tgccagactc aatcaccacg ttacccgagg ttttggcgca gatttcgagc
      121 gcatcgagaa tgggcacacc agactggagc agtgtcccga gcgtgcgcgt gaagcgggcg
      181 acggcgattt tgcgcagcac gtcccccata atcgggagcc ttagcaggag ctgatgaaag
      241 cctcgcttgc ctcgcggcgt gcgcaacaga taaccgaaac ctactgcgaa cgccaagcct
      301 ccaaacatca gcaacggtag cgccgtgacg aacaagtggg agacgtccac cacgatttgg
      361 gtgagcttcg gcagcgcttc ctttccgccg ccgaaatcct tgaacatgtt ctcgaaggcg
      421 ggaatcacga acgtgagcag tacggtcatc acacccgacg cgatcacgat cacgatcgac
      481 ggatagacca tcgcgccgcg cacttggcgg accagctttt gtcgcttctc caagtacacg
      541 gacaagcgat tgaggatgct atccaagata ccgccgactt cgcccgcatg agccaaattg
      601 acgaacagct cgtcgaacac tttgggatgg cgccgcaggg cttcgctgaa cgtagagccc
      661 ccctccaccg caccctgaat gtccttcagc acgccggaaa aatgtcggtt gctttgttga
      721 ccgctcaaaa tttcgaggca ctggaccagc ggtaatcccg cgtcgatcat ggtcgcgaac
      781 aagcgcgtga acgtgaccaa gtccttggtg ccaaccccgc tgcccaactg aaagctcgtc
      841 gcgcggcgcc gcgccacctt caccggattg agctgttgtt gacgtaggcg ctgattgacg
      901 gcctcctcgt tgtcggcctc catcacgccc ttacggagct ctccggtccg ggcgcgcgct
      961 tcccaagtga attcggccat gtcgtttatc tcggcacttc aacgcgtgag ctgcgacgaa
     1021 aggacgtgcc gatgaaacga ccaacgagtg ggtttctgta gccacgcact gcgggatgca
     1081 gctcgccttc caagacagca tggagaatag tcccgccgca cgcgcgtcgt caaaacttcg
     1141 ccgcgctgcg cggcctctcc gcctcggctc ccacccctcg gctcccaccc ctcgatcctg
     1201 cccctcgcct gcccggattg aggccctctt gggtttccgg tggactgcgg aggcggcgcg
     1261 cccctccacg ggatgaccgt tctgccgacg tgtgaccgcc ctgcaggtgt cacgcggtgc
     1321 ggggctcact cagtgggccc gacccaaagc gctcgacgtt cgtaggaagt ggttcgagcg
     1381 agagtgggta cggcgcgacg gagcgtgcta tccagcctcg gcctaggcct gcattctgcg
     1441 ccgcgaccgg gccaccatcc ttcatgcgcc taccccgcca tctattcgga cctcgagcat
     1501 agcggaatgg gagcttttcc aaccgccggc tctcctgcat ccaattccgg tttaacttcg
     1561 accgcacccc atccgctcgg atcccgccgt ggcaatcatt cctccatcga ggcgtgcctc
     1621 acccgcgagg tgatcgaggc gggggtggca cgggcggcct ccgccgggat cgcggtgagg
     1681 gctgggcagg gctggcgatg cgcctcagca catgttggca gtctgcgacc ggcaagtttt
     1741 ggcgcgattg acgaacgtgc ggtttatgat ctcgcgtctg taaccaagcc gttcgtggcg
     1801 ctcgccgccg cacgcttggt agagggtggg gagctgactt gggatacgcc gctctgtcgc
     1861 atgctgcctg aaagttcgga cacaccggtc gcggcggcca gcctggagtt gcttctctcg
     1921 caccgagcag ggctggaagc gcaccgtgaa ttgttcgggc cattgtgcgc gcgccgcccg
     1981 atggatcggc gcgcggcgct gcgggcagcg cttcacgctc ggcgaaccga ctgtagcggc
     2041 cccgttcccg tgcttggctt tccgccggta tatagcgatc tcggatatat tctcgtcggc
     2101 gaggcgctcg cgcagcacca gcaatctggc ttggagcgat tggtccaacg cgaagtctgc
     2161 aagccgctgc agctgaccgt cggctcggcg cgcaccttac tgtccgcaca cccgccccaa
     2221 accgaggagc acgcggccga gccagggatg ccccgcaatc tcgtggggcg ggccagccca
     2281 tttcgcgagc gcgtcgtccc aaccgaagtc gtcgcttttc gcggaggagt ggtgcgagct
     2341 gtcgtacacg acgagaacgc ttgggcgctc gctggccacg ggatggctgg tcacgccgga
     2401 ttgttcggca cgctggacga cgtgctctgc ctcggaaggc gcttgctgga tgtgttggcc
     2461 gggcgagaca tgacgtggct cacgagccca actctcgagg ccatgctggc cgatcgacct
     2521 ggtggcacgt tgcgcctcgg gttcgacggc aaatcaacta cggggtccag tgccggaaac
     2581 cgtgcaagta gccgcagctt cggtcacctc ggcttcacgg ggactagtat ttggtgcgac
     2641 ccagaggccg atgcggtgag tgtcctactt acgaaccgcg tctgtccgag ccgcagcaat
     2701 acgaagattc gtgcctgtcg gcccgtcgtc cacgaggcac tcttcgagct cgcccagagc
     2761 cttcgcgccg gcttgcacta acccgcgccg taacccgccg ctgcgaggcc cgaaccatga
     2821 aactcgctcg atccactcga aagggggctg gtcgtcgccg atcagtcatg acaaattcgg
     2881 agacagccct cgcgacactt gtgcgcgaga gacccagcgc acggaccgat gtggaaactc
     2941 accatcgagg atgatcagtc gaatcgaacc gcagtccatt tggtccgcac gagctacgga
     3001 ctcggacgcg gcgaggacaa tgcgattcgt ctgactgagc gcaacgtttc acgccgccac
     3061 gcgcgcctga accaagaggg gcagcgctgg atcgtcgaag atctttcgag ttacaacggc
     3121 tgcttcgtca acgggcagcg tgtctcggga acgcagacgc tctcgcacgg agacctgatc
     3181 cagctcgggg attaccgact cgtcttggaa gacgagtcgt tgtccaccgg gactcaggat
     3241 atcaccgcaa cggtgcccgc cgggccacgt gctccgagcg gaaccgccgc cgaggatcgg
     3301 ctggtgatgc tcatgggccc gaccccgaac gcgcagttcg ccctcagcaa gaactcgatg
     3361 ctgatcggcc gcggcgagga ctgtgatatc tcgatcaatc acccttcggt gagccgcgtg
     3421 catgccgagc ttcatccggt cgcggatggt cgctacgaga tcgtggacaa gggcagcgcg
     3481 aacgcggtgc gcgtgaacgg cgcggagctg ccgcgctccc tgctggatgc ccgcgacgtg
     3541 gtcgagctcg gggacgtggt tttgaagttc atccccgcgg gtgagctata tctaccgggc
     3601 ctagatgact cgcaaatcgt gggcctgagt ggcatcacgg agaagcctgg tccgcccgac
     3661 gcgcccgaga agcgcagtaa gcccttctgg tcgcgctggg gttggtttgg aggctccgcg
     3721 ctcctgttgc tgggcgtggt ggtggtcgtg cgcggtcgca gcgacggccc tcgagacctg
     3781 gtgagcatca aggatgaagc cacggcgcgg gccgaacgaa tcctggtgga ggccaaagcc
     3841 ttgatggcgc gtggcgattt gcgagcggcg cagcgcaagc tcagtgagct accggtggac
     3901 gccccctccc gcgcctcgag tgactttcga accattcaag cggcgtacgc cgactacttg
     3961 ttcacgctgg ccgaccagac ctcggagccc cgcgagaaga gaaatctcta caacgagatc
     4021 gcgcgatcgc cgagcatcga tagcgcgcgg cgcagccggg ccgaagaaca gcttgcgcag
     4081 ctctcggaac cagctctgaa cgtcgctgac ttgccctcgg cgcgagagcg cccgccggct
     4141 cctccggcgg catcgggcga gaacgtcgcg ggaggctccg ccctggccgc gcgctttgcc
     4201 gagccagggc aggctgacgc accggcgcgc gaggcgacac ccccagcagc cacggggtcc
     4261 gtgaaccgcc cggcggtcgc ggtgcggcca accacgagcg cggcgcgacc cagcgggcaa
     4321 cgcaagtcgc ccaacggtgt tccgaccgtc atccgcgaca atccctactg aggcgccgcg
     4381 gctccgctcg tgcagaagtc tacggaacgt gcgggcgagg ctgcgatgca gcccgtcgaa
     4441 aagccttgga gcgtgctcgc atcacgaccg gcactcagcg catacggcgt cctcgcgctc
     4501 gcgctcgcgc tcgagttagt gcccggcgcg cagcgcctgc gcgtactttc gcgccccccg
     4561 gagccggaca gcagcgtggc gcgctccgcg ctgccggaag caacggccac acgtgtcggc
     4621 gaggccgagc taaagaccga aagcgaaggc agcgccgctt tggcgccgct cgcgctggcg
     4681 ccagcggcgc aacaggccgc acgcgccgag cccgagccca aggtgccaat cgtggatgcg
     4741 agcggcaagg cgctacacaa gttctaccgt gcgctccagc gcacgcagag caagcaaacg
     4801 aacgccacca cgcgcatcgc tcacttcggt gactcgatcg tcgccagcga ctacgtctcg
     4861 ggcaccctcc gccggcgttt tcaggaagcc ttcggcgatg ccgggcacgg cttttcgttg
     4921 attgccaacg cctggcctgc ctacttccat tcggacgtgg agcgctacgc gaccgcaggc
     4981 tggaaggtca gccgcgtggt gggtccgctc gccgaagatg ggctgtacgg tctcggctgt
     5041 gtgtctttca aggcggagaa gaacgcgctc gcccgcttcg gcaccacgag caagggggac
     5101 ttcggccgcg ctgtttccag gttcgaaatc ttctacttgg aggagccgag cggcggcgcg
     5161 ttccaaatct ccgtggatgg ccaagtgcgt gaagtggtcg acacgcacgg tccgagcaag
     5221 gcttcgcgca cgcgcgtaat ccaggtgccg gacggcccac acgagctcga gatcctgacc
     5281 aaacaaggca cttcgcggct attcggggtc gtcatggagc gagacgctgc gggcgtcgtg
     5341 ctggacgcga tcggtatcca aggggcgcgc attcgctttt tggacaagca agaggatgcc
     5401 cactacgccg aacagttgaa gctccgtaac ttcgacctgc tggtatacga gttcggggcc
     5461 aatgaaagcg ccgacggctt cctgtatccc atgccggagt accacgacac gatgaagcag
     5521 gtcatcgcgc aaggtcaggc cgcactcccc gagtccagct gcttggtgat tggagcgatg
     5581 gaccgagcgg tgcaaaaagg cgatgaattg atcgggatcc gggtgctgcc cttcttgatc
     5641 gaagagcaac gcaaagttgc ggcggagctc ggctgcggtt tcttcgacac ctatacggcc
     5701 atgggcggcc gcggctcgat ggcgaagtgg gtcaagagag gcctcggcca ggcggacctc
     5761 acgcatccca ccgccgtggg ctcggacgtg atcggaacct ggatctatcg cgcactatgg
     5821 cgcggctatc aggacttcct ctccactcaa aagcccagcg ccccgagcag ctctgccgat
     5881 agccgttctc cgagctcgcg atagccgtct ttagtgaggt ggatgcgatc ggcctgagcg
     5941 aaacgaggct cggtgcgttg ccaggcggcg aagcttccgg ggccacccat cgcagcctgc
     6001 aagctgtaga aggcgcagcc caggcgagcc gcggtttgag cgtaaactcc ggcgatctcc
     6061 gggatgcgag cgaccgaacc gccctcgacg tccaatgcgt cgggcggtcc gagcactaga
     6121 cactccgagt ccggcgctgc gcgtcgagct cggcccaaaa gagatgaaag ctcttgatcg
     6181 tacgccgaaa cccgcagcgc atcgaaagct tcgttggtcc caaaagccaa cacgatcaaa
     6241 tcggggcggc gtgcagcgag ctccgcgatg aacgattctt cgtcccacgc gagcgcggtc
     6301 gcgatacggg cgccatcgat accaaccgta tccagaacga cgccgggctg ctcgctttcg
     6361 acgattacac cgtgtagccg cggacgaccc gtttgaatct cgagcgtgag cgtgtccgtc
     6421 ccctgccctt cgagttcgac atgctcgatg ctgcttccgg gcgctctcga gcacgccggc
     6481 gcttcgtgaa gcggcggcgc tcccgcgccg cggtcgctcg ccgccttttg gatctggccg
     6541 tcgtggacct ggccgtcgcg gacaagcacc gtctgcgctc ccaatttcag ggtcaacgcg
     6601 ctcctgccgc gcgattcgag taaaacgtcg aaacgcgcac gccccgaaat tgcgctcggg
     6661 cttgcctgca gcgacaacat ggctggccaa cccttgggtg tgaccctgag accataatag
     6721 ccgaacagcc catcgccgtg tttggagcga cgggctggcg gcatgggttc caccataaag
     6781 cgcccgttgc gcgacaaggt tgcggcgtcg tggcgatggg cggggctgcc ccatcggaca
     6841 aagcctgggc cacccgctcc gaacgcagct tgaagccgag agcgaactcg acccgtcaga
     6901 aagtcggccg ccgtatgcga gtcgccgatc catacgatcc gaaccggctc ctggcgtgct
     6961 cccgtctgta gatgacgaag ccctgcggcg aagcgaggca gctgcaggcc gatcgtcgat
     7021 gactgcgccg cggcaggcgg ggccgctggg agctccgacg acggcaaact cgtagatgcg
     7081 ggtctggctg tcgcggccgc ccacgtcgac gacccggtcg tcgtgattgg gctggacgac
     7141 gggagcgcta cagacccgcg tgaatcactc cgctcgaagg agcttgcgag cgggtcacgc
     7201 ggggatcgcg cgtcagccgc atttccggca caaaacaaag tcagtgcggc gaacggcaga
     7261 accgccaccg ccgcacgcaa ggctcgccaa tcaagtgacc gcaagcgggg gatccacctc
     7321 tggttcggaa gacagtcgct catcttgcgt gcggattcgt agttgttcga gcagcggatc
     7381 caggtagcta aatagtgagg gacaaggcgg agcaccgcga gccaaaagct cggcatagcg
     7441 ggtctggtcg tactctgctg cgggcgtcaa agcggaccag aaactacggg ggtgttcgga
     7501 caccaaacgt gccgccggat tgttcaaaag cgcgcgggcc catgccaggt tcgagttcgc
     7561 ctcctgcgga agcttgccga gcctctcgag cgcatgctcc cagagctctc cgacgctgag
     7621 gccgtgcggc tcgaagacat ggacagtgtg gcccggctct gccaagcggt ggacccaggc
     7681 tccgaagcgt gcaaggtagt cgaccggcgt cactgcgagc ctcgccttcg agccaggcaa
     7741 tgcgggcaat cgcgcgcctt cgcggctgtt tacggccaaa gccagcagca ggtgcggcaa
     7801 cgtcggccgc gcgaacgcac cggtccgact gtctgcgatg aggtgccccg agcgtaacac
     7861 ccagggttgg attcctgcat cggcgagcat tctctccgtc aaagccaaag tacgctccaa
     7921 cggattgcgg aaaccctgcc ctgccacgag ttcgtcctcg agcactcggc cggtccgatc
     7981 gccgctcacg aaaacgctcg agtagagtac caatgtccga agccgagagg ccactttcga
     8041 aaactcgacg agctctcgag cttgcccaat gtgtagcgcc tcggccgtag cttcgggaag
     8101 gtcctcgtcc aaactcgagt aggccgcgtg gatggcccgc gtgcactcgg cgagctgtcg
     8161 gtagcgcgat ccggacaacc cccagtcgat cgcgatcgga tcggctggtt cgagcgtcac
     8221 gcgctcacgc gccgcttcgg accacgacgc gatctgcgtc tgcgcctcct gcagccgctc
     8281 cggatgtacg aggagagtga gtcgtgcgac tgcacccgca cccccgtcca gtaactcccg
     8341 ggtaatgtga acggcgcgct gccccggaaa gccaataacc agcaccgcat cggctagttc
     8401 agtcacctgc ccatcgcctc cttccgccct ccttggctcg ggcaggatta cctttcacgg
     8461 acccttcccc gcattggatg ggaaagcgcc gggcaagagc tgtgctcgac gagcgagttc
     8521 gatgagtgcg gctgagaaag atccccaaag cgctgggctt ccgcgctcca atccaaactc
     8581 cacgaagccc ggggcaggcc gcgcactgcc gccgagtgca gccgggttca ggtaaagcgt
     8641 cgcgatcgct tcttggggct gtcgcatcca gttcgaaacc catggctcac gactcaaagc
     8701 aggcccatcg gcagccgggc ttccgccggc ggggaccgct gccgacgcaa accagtaccg
     8761 gtcggaacca acctccacca ccaactgttg cgtttgggca cgatcggtgg tcgcgaagct
     8821 ggcacggaat tgagtacccc ccggtgattt gcgggcagga atcagagtcg gcttcccgag
     8881 cagagcactg agcgggcctt ccagtggagg ccgggagaat ccagaaaaca gacccttgag
     8941 gccgcgctcg agcaaacttg cgtcctgcac ttgaccggta ccgacccaaa tgcgtcccga
     9001 acggagcccc agcgtttgct cccccgtcaa agctcctgcg acgtcgttcc acgctcgctc
     9061 gagctgggcg atctgttgag gcgtcgcgcg atggccccat atccccagcg cggggccggg
     9121 ctgctcggca ctcttgcccg cagcgggctc ttgccaaagc ccctgcaaag aaactgcaaa
     9181 ggccgtgtcg ctcggcaaag accaaatcaa agaaggcacc gccaccgtgc ggtccttcaa
     9241 cttcgaccaa aggggtgatt gcccttccat gaaggcatgc cacttcacga actcgccatc
     9301 gcgttcgacg tcgaatacaa gccccgacgc ctcgcccacg aactcccgga cggtttccac
     9361 gacgcgattt agtttcgcca acgctcccgc gggatccgcg aagtctgcgg ggcgtccgcg
     9421 gtgaagttca tcggaacggg ctgcactttc cagctgagca cgcagcgccg accaacgctc
     9481 cactagcagt tcgttcatcc acgacctgag ccccccgcgc gtcacgcgac cgcggacagc
     9541 gcctgcacgc gccacgcgtt gcgctacacc ctgtgctgca tagggcccaa tactcgaatt
     9601 gagctcgcaa ccgccaaaga cggcgacatt cccgaagacc gtcacgccca agggccacct
     9661 gggcttcgaa gttccgatcg acggcgaagc cgcttgcggc ttggaagtgc cggtggcgga
     9721 ggccgccgac gaagaagcca ccaagcacgg ctgcccgcgc acgatctcga cgtgatgaga
     9781 cggcgagcta cccgtagtca gctgcgcggc aagctcggcc ccgctgcgga cacgcgccgc
     9841 aaacacccaa tccccggttg aggttgcctc gtcgttctgc gcgtaagccg caacgaacgg
     9901 cgcttccaca tcgaacgagg tcgccaccaa aggtccgaaa cccagcacgt gaccgagcgc
     9961 caactcggcg gagcgcggca gctccggcgc ccaaagctcg cgaatcgcgg cgtagacccg
    10021 ttggggttcc ggaacttcga gttcgaacaa caccgtggct ggtcgctcga ctggtgtttc
    10081 cggcaacgga gcagtactgg ggcgttcact cggtcctgcc ggcgccggct gcctcttgca
    10141 agcctctgcc ccgagcaatg ccaaaaacat gcacgcgagc caaccgagcc cgcgtgcacg
    10201 ccacgcgaca cgccgtagcg actcagaacg gaatgtcgtc gtcgccgcct ccgtaatcgg
    10261 agtcgtcgcc accgccacct ccacccgggc ccatctcctc gccgggacca ccgtaatcgt
    10321 ctgaagcggg ccctcggccg ccgccaccgc caccgccgcc accgcgcgcg ccgccggcca
    10381 aaatcaggtt ggtcgccacg acctcggtac gataccgctt attaccgtcg cgatcgtcgt
    10441 agctggacgt cctcagccca ccttcgacga agaccctgga gcccttgctc aggatcttat
    10501 tgagcgcctc cgctcgtttg ccccacagca cgacgctgtg ccactccgtc ttttcctgcc
    10561 gcactcggtt gcggtcgaga taactctccg tcgtagccag cctcaacttg agtacggctt
    10621 gacccccttg ggtcatacga agctcgggat cggcacccaa gtttccaagt agcattacgc
    10681 ggttcaggcc gtcggccatg acagggaact ccttctttgg acctacgcca tgtgcccagc
    10741 catccgaggg aagtcaagcc agctcggatc tctgctgtcg tagcgcgcct gggctcagcg
    10801 ccgtgacgag aatgagagct cggcgagcat cgcccggacc tgctcacgca gcgcgcaaag
    10861 ttccgctcgc gcgtgaaggg gtagcgcgtc gggacgccca tctcgctcaa ccgggatccg
    10921 gcgggggtcc cgcgacgctt cgaccccggg ctgcgccggc gcaacaccgg gttgtgcggc
    10981 ttcgattccg gagtgggcct gcgtcccttc cgcgccaatc ctgttttccg gcgcaccctc
    11041 gagttgaatc actgcgcgcg cggcgggggc ggggctcgcg ttggcgaccc gcgagccagc
    11101 catattgcca gctagcaact gagacagagc gaacggggca acttcgtcgt gccctgcgtc
    11161 gacgaccgcg gatcgttccc tggtcagctc ttgtgcatct cgtgcgtggt caagagcggg
    11221 cgaatctgca gccagctgcg ccggctgagc cgagccctct gacggttcgc ttccgggact
    11281 caagtctggg ccgccttcgc gcagccgtcg acgtgcaccc gcgatcgtga agccgtgagt
    11341 gtagagcagc tcctttactt tcaggagctt ctcgacgtcc cgtcgcgagt agacccgctg
    11401 acctttgcgg gacttttggg ggcgaatcga gcggaactca ccctcccaat agcgaagaac
    11461 gtgcggctct accccgacga tctgcgcgac ttcgccgatc cggtaataga gctttacggg
    11521 aagctcggtg tcaccagcca cgcctactca gctttccgag tcactcaacg ggaggcttcc
    11581 ggcgacaccg cagctgccgc ttcggcactc ggcacgtcgt cgttcaaagc ttgcttgagc
    11641 agctggctcg ccttgaaatt cagcactcgc cgctcggtaa tcacgatcgg atcacccgtc
    11701 tgcggattgc gaccttggcg ttggcgcttg tctcgcaaca cgaagttccc gaatccggaa
    11761 atctttattt tttcgccacg ccccagggtt tccttcatgg tctcgaatac gaggtccacg
    11821 aggtccgcgg cctccttctt cgagaaaccg ccaagccggg tgtagatcgc ctgaacgata
    11881 tcggccttgg tcatcggggt gtgactggct aactagccgg taaaacgaaa actgctcttc
    11941 ccccatgcag caatgcgcga gggaagagca gtcagcctac cggatcatat cgggaagtcc
    12001 atatcgaact gccagacgtc tccaccaacc cggctcattt agagcgggac agggccgggc
    12061 gagcaatcac tatgccgcgc ggaccatctc ccccgactcg cggctcgcat ctgcaccggc
    12121 ttcgagcggc acgcccgcgg gggccagctg cggggcggaa cgcgcaccca agacctgttc
    12181 attgacgacc agcacggcca cataagaata gcctgcacag aacaaggctg cgaacggagc
    12241 ggccagccag tgtccggtcg tcagggcggc gaacacgctc gcggcgctca cggccgccag
    12301 gaacatctcg gccaacggca gcttgttgta ctggcggtac cgcccctgag cacttccccg
    12361 cttgggcgtt ctcacgaact caccggcgtt gaaccgcaag ccgtcccaaa ccgcggacga
    12421 taggtgcggg gccaagcctg cgcccaaagc aattagcatc ggcagccgac gaatagcttc
    12481 ccaagccgag cggccctggg ccacctcggc catcgagtag aagctcgcca gggagcctgt
    12541 ggccccaatg cagagtggca gatccacagc aatcaaggtc tgaatgttgg tggccggcat
    12601 gaacaccagg gccggcaaca gaaccagggt tagcagcacc gtcatcgggt aagcgaagtg
    12661 cggcagcatg tggaaggccg cctcgactcg ctgatgaact ggcaaaggcg ccgcccaaac
    12721 ccgtccgagg agcttgcgcg cggtctggac cgtaccctta gcccaccgat actgttgagt
    12781 ccggaaggcc gacatatctt ccgggagctc ggcgggggta cggacgtcag cccggtagac
    12841 gaagcggtaa ccaatcaact gagcccgata agaaaggtcc aggtcctccg tcagcgtgtc
    12901 gtgctgccag cccccggcac gatcgatcgc ctcgcggcgc caaatcccgc ccgtgcccga
    12961 gaagttgaac atgcagccgt tcacaaaccg ggcgcggttt tccactagat ggtggccatc
    13021 tagcatcagg gcttgaatcg aggtgagcag cgaagctgac cgattcaagt gatcccagcg
    13081 cgtctgcacc atgccaatct gcggatcctg gaagtggccg accacttcac gcagaaacga
    13141 gggttgcggg atgaaatccg catcgaaaat ggcgacgagc tcgccgcgag cgctcttcag
    13201 accgtggtcg agcgcgccgg ccttgtagcc gtgacggttg ggccgccgca cgtaatgagc
    13261 ctgaattccg cgagccgcga gatcgcgcac ggccgcttcg gccagcgcta ccgtctcgtc
    13321 cgtggaatcg tcgagaacct gaatctcgaa acgatcccgg gggtagtcca tcgcgcccgt
    13381 ggactcgatg agacgccggg cgacagttgc ctcattgaag agcggcaact gcaccgtcac
    13441 gaagggcaga gcctcttccg aaacgtcgat ccgctttgcg agccggtcga tgcgccgagc
    13501 gtggcgcatg caggtatacg ccagatgggc gcgatgaagg ccatatgcgg ccagaaccaa
    13561 gagcacagag caataaagaa tgaccagtac ggggtacatg gctgtgaccc ttgggtacca
    13621 ctcagccctg ccccaaatgt cacgcgaatg taattcgttg catcttgatg tcgggctgag
    13681 cccggttttt gcgggaaatt tggcccattt gccacccggg cggcaaaacc ccaaggtttg
    13741 acatgcggta atttgggccg gaaatgatga ggtcgatggc gtccatcaca tcccaaagcg
    13801 gctggccccc gggcgtttgg ctggagattc acggaccgcg ccggctctac tttgctcggc
    13861 cggactcggg tgttgccgga ctccgagccc cgagctcacg cggccgcgga ttcggtcaca
    13921 agcgcgctcg agggcggagt tccgaccgga atgcggatct cagccggggt gcagcaactc
    13981 acgatctcga gattgagact ccgcacatag catctcaaat ttgatgttgt cttgaccgaa
    14041 tgttgcggcg tccagtgcca gagtcgaacc ggtatgaacc caagctgggt acgggtcccc
    14101 tctggagcaa cgtccatccc accgtcgcag gtagcgcacg agcggcgtcg gcggtcatga
    14161 tgctcgtcgt gttacgaccc gctcgggtat gacccaaacg ggttgtccat agatgcgcca
    14221 gggagcgatc cgcacgcgct gggtcgtgtg agcccgtgcg cgtccctgaa tgcggttgag
    14281 catcgcggga tgcggttgag aatgtgcagt ttcctcttcg tggcacgtgc tgggactcga
    14341 gcccccaaca gctgctcaag cgggatcgct cagaagcagg aaaattgcac cgcggacgat
    14401 gaagctgccc ggctgccaat ccctggcctc atttctgagc cgttcagcgt ccagggcgta
    14461 aacgcccgaa tctccgagga cccctcaagc aagcg
//
LOCUS       DASTKU010000218         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_710173, whole genome shotgun sequence.
ACCESSION   DASTKU010000218 DASTKU010000000
VERSION     DASTKU010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710173"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="VFQ61_08240"
     CDS             <1..1133
                     /locus_tag="VFQ61_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET9954479.1"
                     /translation="DAGPQKPDMTVYPAMPPADGSIDSVAISNPLQAKAMKYLNRGYN
                     ITNWLEQGRFNGFTYDEQFVAKLAAAGFKGIRLPVDLDLYVSAKAGSGDETSVVVDAD
                     LFTVLDSFDAWTRTYGLSLTIDYHQYSTLPNKADADSVATMVALWGEVAKHFAGNPRE
                     DLFFELVNEPEQTFDGTDPTAAEWGAIAERMIRKIRESDSAHSIIFGDVEWYGIGPLS
                     KRTPFSDANVIYAFHDYDPFIFTHQGASWANMGATHDLPYPYDPARWSAFYSDLGFNA
                     SAEAWLLTAVQNYYREGNRSALRNKILNAKRWAVSNNVPVICNEFGAYDATSKLEDRA
                     RYYADLVSIFKELEIPWQHWFMVMDGSGNVIPEYRAAMQLDE"
     gene            complement(1174..1995)
                     /locus_tag="VFQ61_08245"
     CDS             complement(1174..1995)
                     /locus_tag="VFQ61_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954480.1"
                     /translation="MVHLENIDKQGGRNGPDTSEVDAGWDFEPASQAVAAESVDSLRP
                     SYASRPPSNAPFRVAGVQQRRQLLRMGGLLLTTACCVGGLAIVGYGRPKPRVATRPPA
                     SPVASATTARGEPSMIRELPSPLPAVSLDVSARPLQARSVRLKVEPPDATVDVRGKRV
                     GGDLALELVEQEGERATVSRPGYRTRIVEIDGSVDCVEIRLERLPRLRRTPKPRSLTN
                     AEAERGGEPRNASNNEESHEQPSEAERVYPAEPSDAPAPGAPRTDDSPSQSAQGQ"
BASE COUNT          393 a          756 c          706 g          418 t
ORIGIN      
        1 aagatgccgg cccccaaaag ccggacatga cggtgtaccc cgcgatgcct ccagcggacg
       61 gcagcatcga ttcggttgcg atttcgaacc cgttgcaagc caaagccatg aagtacctga
      121 accgtggcta caacatcacc aactggctcg agcaggggcg cttcaacggc ttcacctacg
      181 atgagcaatt cgtcgcgaag ctcgctgcgg ccggcttcaa aggcatccgc ttacccgtgg
      241 acctcgatct gtacgtgtcg gccaaggccg gcagcggcga cgaaacgtcg gtggtcgtcg
      301 acgccgacct gtttacggtg ctcgactctt tcgacgcttg gactcgcact tacggcttgt
      361 cgctcacgat cgactatcac cagtactcga cgctgcccaa caaggcggac gccgattcgg
      421 tcgcgaccat ggtggccctc tggggcgagg tagccaagca ttttgcaggc aatccacgcg
      481 aggacctgtt cttcgagctg gtgaacgagc cggagcagac gttcgacggc accgatccca
      541 ccgctgcgga gtggggcgcc atcgccgagc ggatgatccg aaagatccgc gagagcgact
      601 ccgcccacag catcatcttc ggcgatgtcg agtggtatgg catcgggccg ctcagcaagc
      661 gcacgccctt ctccgacgcg aacgtcatct acgcgttcca cgactacgac ccattcatct
      721 tcacccacca gggtgcatct tgggccaata tgggcgccac gcacgacctg ccctacccgt
      781 acgaccccgc gcgttggtcc gctttctatt cggatctcgg tttcaacgct tcggcggagg
      841 cgtggctctt gaccgcggtg caaaactact accgcgaggg gaaccgctct gccctgcgca
      901 acaagattct gaacgccaag cgctgggccg tcagcaacaa cgtccccgtg atttgcaacg
      961 aattcggcgc ttacgacgct acgtccaagc tcgaagatcg cgctcgctac tacgcagatt
     1021 tggtctcgat cttcaaagag ctcgagatcc cctggcagca ttggttcatg gtgatggacg
     1081 ggagcggcaa cgtgatcccg gagtatcgtg cggccatgca gctcgacgag tgacgctgcg
     1141 aggcgctcgc cctgtttcgt gacgggctcc gcgtcattga ccttgtgcgc tctggctggg
     1201 gctgtcgtcg gtgcgcggcg ccccaggggc gggggcgtcg gaaggctccg ccggatagac
     1261 tcgctccgct tcactcggtt gctcgtgaga ctcctcgttg ttcgatgcgt tgcgtggctc
     1321 tcctccgcgc tcggcctccg cgttcgtgag agagcgaggc ttcggggtgc gacgcaggcg
     1381 cggaaggcgt tccaggcgga tctccacgca atcgacgctg ccgtcgatct ccacgatgcg
     1441 cgtgcggtat ccgggacgag atacggtcgc gcgttcgccc tcttgctcga ctagctcgag
     1501 agcgagatct ccgccaacgc gcttgccgcg aacgtccacg gtcgcgtccg gtggttcgac
     1561 tttcaagcgc acgcttcgcg cctggagcgg ccgagctgac acgtcgagcg acaccgctgg
     1621 cagaggcgaa ggcagttctc gaatcatcga gggctcgccc cgcgccgtcg tcgcgctcgc
     1681 aacggggctc gcaggcggcc gtgtcgcaac gcgaggcttc ggacgcccgt agcccacgat
     1741 agccaacccg ccgacgcaac atgcggtcgt gagaagcagc cctcccatgc gcagcaactg
     1801 ccgcctttgc tgcacaccgg caactcgaaa cggcgcattg ctgggcgggc gcgacgcata
     1861 gctcggcctc agcgagtcga cactctccgc tgcaacggct tggctggcgg gctcgaaatc
     1921 ccaaccggcg tcgacctctg atgtgtcggg accgttgcga ccaccttgtt tatcgatgtt
     1981 ttccaaatgc accacgcccg gtgagcacgc tcgcaccggt gaatggtcgg agctgggtca
     2041 gttttctgat ccgagcgagg ggccgcatcg cgctttgctc cgctgacgct gcacgagcca
     2101 ctccccttgg taccgctggc gccagggggt gcccctcccc gaggtcatcc cccacaaagg
     2161 agcgcctgaa agaagctccc tcgagtcagc ctgcgccgct cacaggcatt cagcgtcgcg
     2221 agggcggctg cgcgccgggc ggttgtgctc cgggtcagtg cggcggttcg aag
//
LOCUS       DASTKU010000219         8575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_489989, whole genome shotgun sequence.
ACCESSION   DASTKU010000219 DASTKU010000000
VERSION     DASTKU010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489989"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VFQ61_08250"
     CDS             <1..727
                     /locus_tag="VFQ61_08250"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9954481.1"
                     /translation="LRGSAEADLGFLSAALSNASGAVRCAAVEALTDLGGARAAEAVA
                     FASTDEEPEVCRAAVAALGRMRSEHGTAVGLPRLVELVERSTDPELLEFAIRALSETH
                     APEAAEVLRPLLRSSQPLLAVCAVEALAELSVESAADALLDGLQHAEPEVVKAAMQAL
                     SERPSPQAIARIGACLEHEAWDVRRLAADLLGRVSGDIALGLLRARLAREDSPTVQAA
                     IGRALERIAGIRRTPPPGSFRQR"
     gene            724..1608
                     /locus_tag="VFQ61_08255"
     CDS             724..1608
                     /locus_tag="VFQ61_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET9954482.1"
                     /translation="MRRFESDARPHLSPDDFRLMRDLVYAHSGLRFEEDASLLFERRL
                     AERVEALGLPSFGAYYKYLRFNQAGAAELEEAVERLTTKETYFFRQEYQLRAFRDELL
                     PMLAQQNRDSKRLAVWSAGCSTGEEAYTIAILIDQSGLFHSWDVRVVGTDISKQCVAH
                     ARRGVYKASSFRTTPPEVRARYFREQPDGSHVADEVKKLCHFGNLNLLDHSRAGLVGR
                     VDVVFCRNVLIYFDVQSRRRVIDLFCERLLPGGYLLLGHSESLLNVTTAFELVHLRED
                     LVYRKPPTAERWDRGRNG"
     gene            1605..2657
                     /gene="cheB"
                     /locus_tag="VFQ61_08260"
     CDS             1605..2657
                     /gene="cheB"
                     /locus_tag="VFQ61_08260"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HET9954483.1"
                     /translation="MTADSPIRVLVVDDSVFNRRSISDILATGPGIEVVGKAADGEEA
                     LRLVSALKPDAITLDLEMPRMDGFTFLRILMTKFPTPVIVVSSYSQKENVFKALELGA
                     LDFVAKPDRYGDPELTAIREELVRKVLLVRSVRRLAQTPTRRTPDVQAQRQPELVSEP
                     VVAPKFVVAIASSTGGPSALMEIFARLPQSYGSGIVIAQHMPDKFTRTFAERLDRRSA
                     LFVREGIERETLGAGMAFVCPGRQCMELESFSRGLRVRVLAPTDQDRYLPSADRLLTS
                     VAAAAGRRSIGVILTGMGDDGVIGARAIAAAGGTVIAESESTAVVYGMPGAAVRAGVV
                     NLSLSLPEIADYLARL"
     gene            2790..4259
                     /locus_tag="VFQ61_08265"
     CDS             2790..4259
                     /locus_tag="VFQ61_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HET9954484.1"
                     /translation="MTKRTALSSLILFSALLSGSCARVAATHADAAPEPPRTQLPVTV
                     QQNPAFGKSGALSIADVAERALPSVVNIALTKQVQGPGPEMQFPFPFFFGPPQERGER
                     RQHGLGSGVIVSADGYVLTNNHVVEGASEIKVTTSDKREFDAQVVGNDPKSDLAVIKL
                     NGNVTGLKPIEFGDSSKLRLGDVVVAIGNPFGIGQTVTMGIVSAKGRADMGIVDYEDF
                     IQTDAAINPGNSGGALINMEGQLVGVNTAIYSRSGGYQGIGFSIPSNMAVPIWESLKK
                     NGRVVRGWLGVGIQDVDADLASGLKLPSSRGVLISDVRPGGPAARAGLRRGDLITKID
                     GRPVASSGEFRNLVAASGASRSVNLEMYREGKLQTLPVMLGEMPNDTENSAAAPSGSG
                     PSASAALSGLVLEPLNAENRRAFGLPPQVQQGVVITQIAPGSSAARAGLRPGDVVLEV
                     SGVTIDSLQKFQDAFAKAKGSPVLLVQRGGSTVYVPVRR"
     gene            4400..5989
                     /locus_tag="VFQ61_08270"
     CDS             4400..5989
                     /locus_tag="VFQ61_08270"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9954485.1"
                     /translation="MPELRPTSTPASQRSPSTGVEAAASSGPATIHNPRSCPHCGGRY
                     PADFKVCPKDATPLEDAPSDIDDPVVGQVLSGTYEVLRVIGEGGMGRVYEARHSRLGK
                     KRFAIKMLHHELARQAEVVTRFQREAEAASALSHPNVVTVFDVNTAPDGRPYIVAELL
                     EGEQLGEHLERRGKLPVREAVSIVRQICRALERAHDLRIIHRDVKPENVFLVSGLSEV
                     KVIDFGISKVSDGTATLTKAGMVMGTPDYMAPEQARGDTVDARADVYAVGAILYRAVT
                     GRKPFEGLDPMATLTAAMVQEPPRPSTLEPSIPLSLELVIQHAMAKKANERFQSMVEL
                     DRALEAFDVPDAAPPTERKASVDASGKTILSAAERSETSQAIAERSGDRARLARPSLS
                     FFTGLGFIWLVVNVIVAGSAVLRLLQGSVSGSEIVVILAAALAAAATPFAIWVRFVAR
                     EVWPSTPKSLEMGDRLRRTVLFSATAYGLAALFVQIFETAGKAAQGPGPISDPHWALM
                     QFSVALVTALITWFVGTPRRR"
     gene            complement(5986..6408)
                     /locus_tag="VFQ61_08275"
     CDS             complement(5986..6408)
                     /locus_tag="VFQ61_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954486.1"
                     /translation="MSLPEGWGSLRSKVAAAALLLGIFVAGAFTGVAIYRWARHDDLH
                     HLHPRPHPDFPKMLADNLDLSPAQEQQVREVFDRHRPELDAVFRETFPRVRAVQDSID
                     AEVRPLLNESQRQRFDEFKARANKGPFPPGPFHPGPPR"
     gene            complement(6395..6724)
                     /locus_tag="VFQ61_08280"
     CDS             complement(6395..6724)
                     /locus_tag="VFQ61_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954487.1"
                     /translation="MSDPKLDLSALAATRSLEERIGELVQRAVRLRRQELGWVEQLER
                     WLRPALALAATVALVSWLGVSRREPSSELPSHSDPAISLLSWVNQTHPPAASEVLQSF
                     GPSHVAP"
     gene            complement(6721..7377)
                     /locus_tag="VFQ61_08285"
     CDS             complement(6721..7377)
                     /locus_tag="VFQ61_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9954488.1"
                     /translation="MVLAYPAASAMLEFLQREAAAAAPSPRTTAISTHSLDPKAVAEL
                     VNAARRGEAHALEALARAYLRPAYSVALAILRRPADAEDAAHDALLTAFERLDSCREP
                     ARFAAWFMTIVRNHSKNQLTRRKLRDVPDEDTDFDPGTTHGAPQLGVLRSRLLRALGQ
                     LSPVEREVVLLHDLEGLTHPEIADLLDVSCVMSRQHLFVSRRKLRHFLGDELDDGDSP
                     "
     gene            7641..>8575
                     /locus_tag="VFQ61_08290"
     CDS             7641..>8575
                     /locus_tag="VFQ61_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954489.1"
                     /translation="MKRLFRTAPMRTRLIPSVLLVAAWMASPLSVQGKPDDRGSAKAA
                     PAQPKKADDAPVKSQEARGNEARAAGAKTQEVRKNAEGASSPGSLPEATGEPSLDPRR
                     GQGARNVEIEPSPRVGKEGSAKTESGTGKAEPKETRAEAREKDEPSAGSKSAKGKANA
                     KAPRSTPDMNQRRQIALGPTSEELAVGKEDPELRSLRDAELVLFPQPLPGLVPGWSWD
                     LPQPSDGLPEIVSGAPLPAPAVVLPKSSSGSPEWLRSMSMPNLPVRLEDRVVRYLTFF
                     RDSPSGRSIARAWVKKSGRYVAALRAELARNGLPT"
BASE COUNT         1521 a         2604 c         2874 g         1576 t
ORIGIN      
        1 gctgcgcggc tcggcggagg cggacttggg cttcttgtcg gcggccctgt ccaatgcttc
       61 tggggcggtt cgctgtgccg ccgtcgaagc cttgaccgac ttggggggcg cgcgcgcggc
      121 ggaggcggtg gcgtttgctt cgaccgacga ggagccggaa gtatgccgcg cggcggtggc
      181 cgcactgggc cggatgcgat cggagcatgg gaccgcggtc gggctacctc gactggtcga
      241 gttggtcgaa cgatctacgg atccggagct gctcgagttt gcgatccgcg cgctgtccga
      301 gacgcacgcg cccgaggccg ccgaagtatt gcggcctctg cttcgttcct cacaacctct
      361 gctcgccgtc tgcgcggtgg aggctttggc ggagctttcg gtcgagagcg ctgcggacgc
      421 gttgttggat ggcctgcagc acgcggagcc cgaggtggtg aaggcggcca tgcaggcgct
      481 ctccgagcgt ccgagcccac aagccatcgc gcgcatcggc gcctgcttgg agcacgaagc
      541 ctgggacgtg aggcgcttgg ctgcggactt gctgggtcgc gtgtcgggcg acatcgcact
      601 gggcttgttg agggctcggc tcgcgcgcga ggacagcccc acggttcagg ccgcaattgg
      661 gcgcgcactc gagcgcatcg cgggcattcg tcgtacgcca ccgccgggga gctttcgcca
      721 gcgatgaggc gcttcgaatc cgacgcgagg cctcacctgt caccggacga cttccgtctg
      781 atgcgggatt tggtgtacgc gcattcgggg cttcggttcg aagaagacgc ctcgttgctg
      841 ttcgagcggc gcttggcgga acgggtggag gcgctcggtt tgccgagttt cggcgcctat
      901 tacaagtatc tccgcttcaa ccaggcgggc gccgcggagc tcgaagaagc cgtcgagcgg
      961 ctgaccacga aagagaccta cttctttcgt caggaatacc agctgcgtgc gtttcgtgac
     1021 gagctcctgc cgatgctcgc gcagcagaat cgtgacagca agcgtctcgc ggtgtggagc
     1081 gcaggctgtt ccaccggtga agaggcgtat acgatcgcga tcctgatcga ccagagcggc
     1141 ctgttccact cgtgggatgt tcgggtcgta ggtaccgaca tttccaaaca atgtgtggcc
     1201 cacgctcggc gcggtgtgta caaagcgagc tcgttccgca ccacgccgcc ggaggtgcgc
     1261 gcccgctact ttcgcgaaca gcctgatggc agtcacgtcg cggacgaggt aaagaagcta
     1321 tgccatttcg gcaacctcaa cctgctcgat cacagccggg caggcctggt tgggcgcgtg
     1381 gacgtagtct tctgtcgcaa cgttttgatc tacttcgacg ttcagtctcg caggcgagtg
     1441 atagacctat tctgtgaacg cttgctgccc ggtgggtacc tactgcttgg gcattcggag
     1501 tctctcttga atgtgaccac tgcattcgag ctcgttcatc tgcgtgagga tctcgtatac
     1561 agaaagccgc ctaccgcgga gcgttgggat aggggtcgaa acggatgact gccgattcgc
     1621 cgattcgtgt gctggtcgtt gatgactccg tgttcaatcg gcggagcatt tccgacatcc
     1681 tcgcgacggg gccaggtatc gaggtcgtgg gtaaagccgc cgatggggaa gaggcgctgc
     1741 ggctcgtgag tgcgttgaag ccggacgcca tcaccctcga tctcgagatg cccaggatgg
     1801 acggcttcac gttcctgcgc atattgatga cgaagttccc aacgccggtc atcgtcgttt
     1861 cgagctacag ccagaaggaa aacgtcttca aagcgctgga gctgggcgct ctggatttcg
     1921 tggcgaaacc cgatcgctat ggtgatcccg agctgaccgc gattcgcgaa gagctcgtcc
     1981 gcaaggtgtt gctggtgcgc agcgtgcgcc ggctcgcgca gacgccgacg cgccgtacgc
     2041 ctgacgtgca ggctcagcgc cagcccgagc tggtgagtga acccgtcgtt gcgccgaagt
     2101 tcgtggttgc gattgcttcc tccaccggag gtccgagcgc gctgatggag atcttcgccc
     2161 gtctgccgca gagctacggc agtggcatcg tgatcgcgca acacatgccg gacaaattca
     2221 cccgcacctt tgcggagcgg ctggaccggc gcagtgcctt gttcgttcgt gaaggtatcg
     2281 agcgcgaaac gctgggggca ggcatggcct tcgtgtgccc cggccgtcag tgcatggagc
     2341 tcgagagctt ctcacgcggc ttgcgggtta gggtgctcgc cccaaccgac caagatcggt
     2401 acttaccgag cgccgaccgc ctgttgacta gcgttgctgc cgcggccggc cgccgctcga
     2461 tcggggtcat cttgaccggc atgggcgacg acggcgtgat tggggcccgc gccatcgcgg
     2521 ccgccggtgg caccgtaatc gcggagtccg aatcgacggc ggtggtttac ggaatgcccg
     2581 gagccgcggt gcgagcgggc gtcgtgaacc tcagcctttc gttgcccgag atcgctgatt
     2641 acctcgcaag gctgtgaggt cgacgggcgg gggcccgtgc tcccgtacgc gactggaagg
     2701 ggcggcgccg cggagggcgg atgctttggg aatgagatgt ggccctagcg ggttgagttc
     2761 gcgaaactcg ataaggcacc tctgacccca tgaccaagcg caccgctctt tcgtctctca
     2821 ttttgttctc tgcgttgctt agcggcagct gcgcgcgcgt ggcggcaacc cacgcggacg
     2881 ccgcaccaga gccaccgcga acccagcttc ccgtcacggt ccagcagaat ccagcattcg
     2941 ggaagtccgg cgcgctctcg atcgctgacg tcgcggaacg cgctctcccg tccgtcgtga
     3001 atatcgcgct gaccaagcaa gttcaggggc ccgggcctga aatgcagttc ccctttccgt
     3061 tcttcttcgg cccacctcag gagcggggcg agcgtcgcca gcatgggttg ggctcagggg
     3121 tgatcgtatc cgctgacggc tacgtactca cgaacaatca cgtggtggaa ggcgcgtccg
     3181 aaatcaaagt gaccacctcc gacaagcgtg agttcgatgc gcaagtcgtt ggtaacgatc
     3241 cgaagagtga tttggccgtc atcaagctga atggtaatgt gacggggctg aagccaatcg
     3301 agtttggaga ttccagcaaa ctccgtctcg gcgacgtcgt ggtggcgatc ggcaatccct
     3361 tcggcatcgg tcagaccgtg accatgggta tcgtttcggc caaaggtcgc gccgatatgg
     3421 gcatcgtcga ctacgaggat ttcattcaga ccgacgcggc catcaatccc ggaaactccg
     3481 ggggcgcttt gatcaatatg gagggccagt tggtcggggt gaatacggcg atttactcgc
     3541 gcagcggcgg atatcaggga atcggcttct cgattccgtc caatatggcc gttccgattt
     3601 gggagtcact gaagaagaac ggccgtgtcg tgcgcggttg gctcggcgtg ggaattcagg
     3661 acgtagatgc cgatctggct tcagggctga agttgccgag ctcgcgtggc gtattgattt
     3721 ccgacgtgag gccgggcggc cctgcggcgc gggcgggctt gcgacggggc gacctgatca
     3781 ccaagatcga tgggcgcccc gtggcctcct ccggtgagtt ccgcaacctg gtcgcggcca
     3841 gcggtgcttc gcgcagtgta aacctcgaga tgtatcgcga aggcaagctc cagacgttgc
     3901 ccgtcatgct cggagagatg ccgaacgaca cggaaaactc ggccgccgct ccctccggta
     3961 gcggtccaag cgccagcgcc gccctgagtg gcttggtgct cgagccgctg aacgcagaga
     4021 accgccgagc atttgggctc cctccacagg tgcagcaggg agtcgtcatc acacaaatag
     4081 cgccgggaag ctcggccgcg cgggcaggtc ttcgacccgg cgatgtagtg ctcgaagttt
     4141 cgggcgtaac tatcgattcg ctgcagaagt tccaggatgc cttcgccaaa gcgaagggca
     4201 gccccgtgct gctagtgcag cgcggaggca gcactgtcta cgttcccgtt cgtcgttgag
     4261 ctgaacccac cttcaccgag cagaacgtct ccggccacgc gcccgacgtt ctgctctcca
     4321 ggttcacgca aaagcgtttg tgcgttacag cacgctgacc gtatgcggcg gcccattcgg
     4381 gcgctacgat acttggtgga tgcccgaact gcgacccacg tctacgcccg cttctcagcg
     4441 gtcaccctct actggtgtag aagcggccgc ttcgtcggga ccggcgacga ttcacaatcc
     4501 gcgttcctgc ccgcattgcg gggggcgata tcccgcggac ttcaaggtct gccccaaaga
     4561 cgcgactccg ctcgaagacg ctccgagtga catcgacgat ccggtcgtcg gacaagtgct
     4621 ctccgggacc tatgaagtgc tgcgggtgat tggcgagggc ggcatggggc gcgtttacga
     4681 ggcccgccac agtcgcctcg gcaagaagcg cttcgcgatc aagatgctgc atcacgagct
     4741 ggctcggcag gccgaggtcg tgacccgctt tcaacgcgaa gcggaggcgg cgagcgcgct
     4801 ctcgcatccc aacgtggtga ccgtgttcga cgtgaatacc gcgcccgacg ggcggccgta
     4861 catcgtcgcc gagttgctcg aaggtgaaca actcggagag cacttggagc gccgcgggaa
     4921 gctgcccgtg cgtgaggccg tctccatcgt tcggcagatt tgccgcgcat tggagcgggc
     4981 ccatgatttg cgtatcattc accgcgacgt aaagcccgag aacgtctttc tcgtaagcgg
     5041 gctatccgag gtcaaggtga tcgacttcgg gatctccaag gtgtccgacg gcacggccac
     5101 gctgaccaag gcgggcatgg tgatgggaac gccggactac atggcgcccg agcaagcgcg
     5161 gggagacacc gtggacgcgc gcgcggatgt gtacgccgtg ggcgcgatcc tttatcgcgc
     5221 cgtgactgga cgtaagccgt tcgaggggct ggatccgatg gcgaccttga ccgcggccat
     5281 ggtgcaagaa ccgccgcgcc cgagcacact cgagcccagc atcccgctct cactcgagct
     5341 cgtgattcag cacgccatgg ccaagaaagc gaacgagcgc tttcagagca tggtcgaact
     5401 cgatcgcgca ctggaggcgt tcgacgtccc cgacgcagcg ccccctacgg agcgcaaggc
     5461 cagcgtcgat gcatcgggca agaccatcct gtctgccgcc gagcgcagtg agacgagcca
     5521 agctatcgcg gagcgctcgg gtgaccgggc tcgtctcgcg cggccatcgc tctcgttctt
     5581 taccggtctc ggcttcattt ggttggtggt gaacgtgatc gtggcgggca gtgcggtgct
     5641 gcggctgctg cagggcagcg tttccgggag cgaaatcgtg gtcatcctgg cagccgcgct
     5701 ggctgccgcg gcgacgccgt tcgcgatttg ggtgcgcttc gtggcgcgcg aagtgtggcc
     5761 gagcacgccg aagtcgctcg aaatgggcga ccgactccga cgtacggttt tgttcagtgc
     5821 gacggcgtac ggcttggcgg cgttgttcgt gcaaattttc gaaacggcag gcaaagccgc
     5881 ccagggtccg gggcccatct cggatccgca ctgggctctg atgcaattct cggtcgcgct
     5941 cgtgacggcg ctgatcacct ggttcgttgg aacgccccga cggcgctaac gcggtggccc
     6001 aggatggaag ggcccgggcg gaaatggccc tttgtttgct cgcgccttga actcgtcgaa
     6061 gcgttggcgc tggctctcgt tcagcaaagg acggacttcg gcgtcgattg agtcttgcac
     6121 ggcgcgaacc cgcgggaagg tctcgcggaa caccgcgtcg agttcggggc gatgccggtc
     6181 gaacacctcc cgaacttgtt gctcctgagc agggctcaaa tccagattgt ctgcgagcat
     6241 cttcggaaaa tcggggtggg gccttgggtg caggtgatgc aagtcgtcat gccgagccca
     6301 acgatagatg gcgactcccg tgaacgcacc cgccacgaag attccgagta gtagggcagc
     6361 ggctgcgacc ttcgagcgca gtgagcccca gccttcaggg agcgacatga gaggggccaa
     6421 acgactgaag aacctcgctt gcagcgggcg ggtgagtttg attgacccag ctcaacaacg
     6481 agattgccgg atccgagtgg gaggggagtt ctgagctcgg ttcgcgccgt gataccccta
     6541 accaactcac cagcgccacg gtggcggcga gcgcgagtgc gggccgcagc caacgttcga
     6601 gttgctcgac ccaaccgagc tcttggcgtc gcagccgtac ggcgcgctgc accagctcgc
     6661 cgattcgctc ttccaagctc cgggttgccg ccaaggcaga caggtcgagt ttgggatcac
     6721 tcatggcgag tctccatcat ccagttcgtc tcccaggaaa tggcgcagct tgcggcgcga
     6781 cacgaacaga tgctgccgag acatgacgca tgaaacgtcc agcaaatccg cgatctcggg
     6841 gtgtgtcaat ccctccaaat cgtgcagcag caccacttcc cgctcgaccg gggagagttg
     6901 ccccaaggcc cgtagtaacc ggcttcgcaa gacaccgagt tggggagctc cgtgcgtagt
     6961 gcctggatcg aagtcggtgt cttcgtctgg gacatcccgc aacttgcgtc gcgtgagttg
     7021 attcttggag tggttccgga cgatcgtcat gaaccacgcg gcgaaacgcg ctggctcacg
     7081 gcagctgtcg agccgctcga acgccgtgag gagcgcatca tgcgccgcgt cttcggcgtc
     7141 cgcaggtcgg cgaagaatgg ccaacgcgac cgaatacgca gggcgcaaat acgctcgcgc
     7201 caacgcctcc aaggcgtggg cttcaccgcg tcgagccgcg ttgacgagct ctgcgacggc
     7261 tttgggatcg aggctatgag tgctaatggc agtcgttcgg ggggacgggg cagccgctgc
     7321 agcttcgcgt tgcagaaatt ccagcatcgc gctggctgcg ggataggcga gcaccattta
     7381 gctgaagact ggacagtccg agggcgggca atgttagcca cttgccaggt agaacctgtt
     7441 cacccggcga acggggtccg ttcgtccttt gcctatggaa ccagcgcagg tttcttggcc
     7501 gagcggaaat ggctatggca cctgtcgttc ggagtgtcag gcctggctcg ctctccaatt
     7561 cagctcactc gcccgcatcg ccggtccacg gctacgagta tgcaggaaag ggtgctacgc
     7621 ctgcgcccgg gaactgagcc atgaagcgtc ttttccggac cgccccaatg cggacacgtt
     7681 tgatcccgag tgtcctactc gtggccgcgt ggatggcgag cccgctctcg gttcagggca
     7741 aacccgacga tcgcggatcc gcgaaggcgg ctcctgctca accgaagaag gccgacgatg
     7801 ctccggtcaa gtcgcaggag gcgcgcggaa acgaggcgag ggctgccggc gccaagaccc
     7861 aagaagtgcg caagaatgcc gagggcgcaa gtagcccggg gagcctaccc gaggccacgg
     7921 gcgagcccag cctagatcca cggcgcgggc aaggcgcccg taacgtggag atcgagccca
     7981 gcccgcgcgt tggcaaagaa ggaagcgcga agacagagag cggaacgggc aaggccgagc
     8041 ccaaggaaac cagggccgag gccagagaga aagacgagcc cagcgcgggc tccaaatccg
     8101 ccaagggtaa ggccaacgcc aaagcgccgc gcagcacacc cgacatgaac caacgccgcc
     8161 agatcgcgtt ggggcccacc agtgaggagc tcgccgtggg caaggaagat ccggagctgc
     8221 gttcgctgcg cgacgcggag ctggtgctct ttcctcagcc gctgccaggg ctcgtgcccg
     8281 gttggtcttg ggatttgcct cagccgagcg atgggttgcc agagatcgtg agcggcgcac
     8341 cactacctgc gcctgcggtg gtcttgccga agtcatctag cgggagcccg gagtggctgc
     8401 gctcgatgag catgcccaac ctgccggtgc gtctcgaaga tcgtgtggtg cgttacctca
     8461 cgttcttcag agacagcccg agcgggcgct cgattgcgcg cgcctgggtc aaaaagagcg
     8521 ggcgctatgt cgcagctttg cgtgcggaac tcgcacggaa cggcttgccc accga
//
LOCUS       DASTKU010000220         8429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1158976, whole genome shotgun sequence.
ACCESSION   DASTKU010000220 DASTKU010000000
VERSION     DASTKU010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8429
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158976"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VFQ61_08295"
     CDS             <1..884
                     /locus_tag="VFQ61_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9954490.1"
                     /translation="DIRLRADQAYRMNTGSLALMTVPSAKYGTVPLTSVVKTTLGTGP
                     SQINRLGRQRQVTFMANVAPGYGESDVVNAMKKAFADLNASAEYRLVPTGRSKSSSEL
                     GAGFALAFSLSFIFMYLILAAQFESWLYPVIILISLPLTVPFALLSLLLFNQGLNLMS
                     ALGLLVLFGVVKKNAILQIDHTNHLRALGKPRLEAILEANRDRLRPILMTTVAFVAGM
                     LPLALSRGIGSGSNRNISGIVIGGQTFSLLLTLLAVPVVYSLFDDVRQWLARRRQGRA
                     VDRGEAELDALVGTHAE"
     gene            complement(973..1611)
                     /gene="upp"
                     /locus_tag="VFQ61_08300"
     CDS             complement(973..1611)
                     /gene="upp"
                     /locus_tag="VFQ61_08300"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET9954491.1"
                     /translation="MQIPENVTVVRHPLVQHKLTLMRQKDRSTSSFRSLLNETSELLA
                     YETTRDLPLVYQEIETPLARMQAPLLEGKKVVLVAILRAGLGLVEGMLRVLPSARVGH
                     IGIYRDPETLGAVEYYMKLPSELHDRDVIVTDPLLATGNSAVAAISRVKSANPKSIKF
                     VCLIASPEGLTHFHEVHPDVPIFLASVDEHLDEHGYIVPGLGDAGNRLYGTR"
     gene            complement(1667..3034)
                     /locus_tag="VFQ61_08305"
     CDS             complement(1667..3034)
                     /locus_tag="VFQ61_08305"
                     /inference="COORDINATES: protein motif:HMM:NF019570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1688 family protein"
                     /protein_id="HET9954492.1"
                     /translation="MSSKKPRVAALPNDEGRTLGGGVGAAASLEKIASASALTEVELT
                     YLKSPRAIRERAHAVLQLAQANQLAHFALRPERLPELAKQVLRFTLENYGELSRVPYH
                     SRYRHFNAAGVDRVARLDAALVSLSPLERLRAEADLVIVTVLLDAGAGASFRYRESQT
                     GLVLRRSEGLAIASFDWFMSGDLALDRRTPLRVESARLQSLEACDLERAFQVGAENPL
                     LGVTGRTELLQRLGEVTTLGTTAKHDADANSNTAPRPGDFVVQLCASAKHGTLRAADV
                     FERVLSVLSPIWPRHLARNGLAFDTHATAAPALGDVFIHPQVGYVPFHKLSQWLTYSL
                     CEAIERFGLRVEGLDELTGLPEYRNGGLLLDAGVLELREPAEANREHTVSSPLIVEWR
                     ALTVALLDELAGLVRHEAGATPESLPLVKLLEGGTWSLGRRLAEQRRPGAAPPLRVQS
                     DGTVF"
     gene            complement(2994..4259)
                     /locus_tag="VFQ61_08310"
     CDS             complement(2994..4259)
                     /locus_tag="VFQ61_08310"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF005536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET9954493.1"
                     /translation="MTEQAKKKRPKPIVLATHPMPHGPRPPAIHWGAREAMERGPVIG
                     TVTNRAHRNVIGAHSGSYSVYRAVAVAAGALDPLRKADLTNTAPTHPIGPFPQWSEPD
                     RIVSLDPFGALVSDAFASYFEQGYDIRPSIAITKAHLKLPELTEAMAAGRLRADGRIL
                     KADGGVVVVKAAIEPVWHLPGVARRFGCSESDLRSTIFEHTGGMFPELVTRSDLEVFL
                     PPIGGTTVYLFGDVASISDPEKPLAARVHDECSGSDVFGSDICTCRPYLTHGIEECVR
                     VAQAGGAGVIVYNRKEGRALGEVTKFLVYNARKRQVGGDRAEQYFARTECVAGVQDMR
                     FQELMPDVLHWLGIRKIDRFVSMSNMKHDALVRSGIRIVERVPIPDDLIPEDARVEMD
                     AKKAAGYFTEGTVPDPHELQKAKGRSLAE"
     gene            complement(4337..5827)
                     /gene="mmsA"
                     /locus_tag="VFQ61_08315"
     CDS             complement(4337..5827)
                     /gene="mmsA"
                     /locus_tag="VFQ61_08315"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET9954494.1"
                     /translation="MMMNNIRFPSCQSDIGNFVSGAFQPFQSSLPVESPYTGDNVARV
                     AISDAKDVARAVDAASAAFPAWRALPVRERCKPLFGLRELLISRLESCAELVAYESGK
                     TPAEARAGIERGLEVIEFALSWPNMESGGALDVSRGVSCEYRREPLGVTLGITPFNFP
                     MMVPLWMLPITLTAGNTFILKPSEKVPLSAALLAELMHEAGYPAGVFGVVHGGRETVE
                     HLLDHPEISAVGFVGSTGAARAVYTRAAQHGKRALCLGGAKNHLIVMPDADPSLTVRA
                     VVDSFTGCAGQRCMAGSVLVCVGAAAEAGRFIEPIVARAAELKMGTEMGALIDAAARE
                     RLARALARAEEEGARLLLDGRRLKPPAGFERGHWLGPSVIADAKIGMACATEELFGPV
                     LTILQVRTLDEALALERTTPYGNATSVFTQSGAVARYVSERALSGMVGVNVGVPVPRD
                     PFSFGGTRQSKFGHGDITGPQAVDFWTNLKKITTKWALQTDANWMS"
     gene            5899..6642
                     /gene="udk"
                     /locus_tag="VFQ61_08320"
     CDS             5899..6642
                     /gene="udk"
                     /locus_tag="VFQ61_08320"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET9954495.1"
                     /translation="MKALAHGAGKTFLDPGMGNRFVADDGSTQCANRVGLLGVSPLVV
                     GIAGGTGSGKSTVARELVRSLPDHAAALIQHDWYYRDRSHLSEAERESINFDEPAALD
                     NELLVEHLLELKCGRAVACPQYDFVTHTRAKASHHVEPRSIVLVEGILIFAVRELRQA
                     FDLRLFVDTDDDIRLLRRVQRDMRERGRDLSSIESQYLNTVRPMHQLHVAPTRQFAHL
                     IIPEGGENVQALDVIVGRLRYLLARAGSD"
     gene            6767..8236
                     /locus_tag="VFQ61_08325"
     CDS             6767..8236
                     /locus_tag="VFQ61_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9954496.1"
                     /translation="MTKFSWYRSTRIAWIAGFTLFTSAGCMQPVGAGAQQAPSAPSAP
                     SSAGSAAPSASAASAPTPTAAAPVEASEPASAEATTVWQVPVTDQDPARGPKDALVTI
                     VEWADFQCPYCQRVEETLKRVEQTYGSDVRIVWKDNPLGFHEHAMAAAKLGRAVFEKQ
                     GNAGFWAVHKALLERQAELSSAVLEELAIGQGLSASQWRRLAGDSESVFISDSIGAGQ
                     ELGVTGTPEFFINGVSLSGAQPFEEFQARIDAELARAKARVAAGVPRAELYATLMKEA
                     QPAPPPPRKEVAPARKDSPARGPARAKVTLEVFSDFQCPFCKTGRDTLERLEKKHPGQ
                     LRVVFRHLPLPFHEHARPAAEASEEVRIQKGNAAFWAYHDKLFAAQSEPGGLSEDRLI
                     ALAVELGVDRKKLERALASGTHRAAIQYDLDAAAAAGIRGTPSFTINGYFVSGARPLA
                     TFERLIRRAERETVSAAGTAAPRPASVAPAATETPPKAP"
BASE COUNT         1537 a         2650 c         2690 g         1552 t
ORIGIN      
        1 atgacatccg tttgcgcgcc gatcaggcct atcgaatgaa caccgggtcg ctcgcgctga
       61 tgacggtccc gtccgcgaag tacggcaccg tgccgctcac ttcggtggtg aagacgacgc
      121 tcggaacggg cccctcgcag attaaccggc ttgggcgcca acgtcaggtc acgttcatgg
      181 ccaacgtcgc gccgggctat ggcgagagtg acgtggtgaa cgccatgaag aaagccttcg
      241 ccgatttgaa tgcgtccgcc gagtaccgct tggttccgac gggcaggtcc aagtcttcgt
      301 ccgagctagg cgcgggcttt gcgttggcat tcagcctgtc tttcatcttc atgtatttga
      361 tcttggccgc gcagttcgag tcctggctct acccggtcat catcctgatt tcgctgccgc
      421 tgacggtgcc gtttgcgctc ttgtcgctgc tgttgttcaa ccagggcctg aacttgatgt
      481 ccgcactggg gttgttggtg ctgtttggtg tggtcaagaa gaacgccatt ctccagatcg
      541 atcacacgaa ccacctgcgt gccttgggga agccgcgttt ggaggcgatc ttggaggcca
      601 accgcgaccg cttgcggccc atcttgatga cgacggtggc gttcgtcgcg ggtatgttgc
      661 cgttggccct ttcgcgcggc attggttcgg gaagcaatcg caacatctcc gggatcgtga
      721 tcggcgggca gaccttctct ctgctgctca cgttgctggc cgtccctgtg gtctactcgc
      781 tgttcgacga cgtgcggcag tggctggcgc gccggcgcca gggccgcgcg gtagaccgcg
      841 gcgaagccga gctggacgcg ctggtgggca ctcacgcaga gtgagttcgc tcctcagccc
      901 gaggttggcg cggcctagaa accgcccaac ctcgaacgcg gctcgagccg aagccgcttg
      961 cgcggctctg gcctagcgcg tgccgtagag gcgattaccg gcgtcaccca agccgggaac
     1021 gatgtagccg tgctcgtcga ggtgttcgtc tacggacgcg aggaagatcg ggacgtccgg
     1081 gtgcacctcg tggaagtggg tgagcccctc gggtgacgcg atcaagcaca cgaacttgat
     1141 tgatttcggg ttcgcgctct tcacccgcga gatcgccgcg accgcactat ttcctgtcgc
     1201 caacaacgga tcggtcacga tcacatcgcg gtcgtggagc tccgagggca acttcatgta
     1261 gtactcgacc gcgccgagcg tctcggggtc ccggtaaatc ccgatgtgcc cgacgcgcgc
     1321 ggacggcagc actcgcagca tgccctcgac caagccgagc ccggctcgca agatcgcgac
     1381 cagcacgacc ttcttgcctt cgagtagcgg ggcctgcatg cgcgccagcg gggtctcgat
     1441 ctcctgatag acgagcggca gatcgcgtgt cgtctcatac gcgaggagtt cgctcgtctc
     1501 gttcagcagg ctccggaagg agctcgtgct gcggtccttc tgacgcatca aggtgagttt
     1561 gtgttggacc aacggatgcc gtacgacggt gacgttttca gggatctgca aggctcctcc
     1621 gagttacatc aaaccacgaa ctcgcccggg agattagagg agatcctcaa aaaaccgtgc
     1681 cgtcgctctg cacacgcagg ggtggcgcag cgccggggcg ccgttgctcg gccaaccgcc
     1741 gaccaaggct ccaggtgcca ccctcgagta gcttgaccaa gggcaaagac tcgggcgtgg
     1801 cacccgcctc gtgacgaacc aaccccgcca actcatcgag caatgcgacg gtaagagccc
     1861 gccattccac gatcaagggc gagctcacgg tgtgctcgcg gttcgcttcc gcgggctctc
     1921 ggagttcgag cacaccggca tccaggagta gaccgccgtt gcgatactcg ggtaagccgg
     1981 tgagttcatc caggccctcc acgcgcagcc cgaaccgctc gatcgcttca cacagtgaat
     2041 aggtgagcca ctgtgaaagt ttatgaaagg ggacgtagcc gacctggggg tgaatgaaga
     2101 cgtccccaag cgccggggcc gcggtggcat gggtgtcgaa cgccaacccg ttccgcgcca
     2161 agtgccgagg ccagatgggg gagagcaccg acaatacgcg ttcgaatacg tctgccgcgc
     2221 gcaaagtgcc atgttttgcc gatgcacaga gctgaactac gaagtctccg ggtctcggcg
     2281 ccgtattgct atttgcgtcg gcatcgtgtt ttgcagtcgt gccgagggtc gtgacttctc
     2341 ccagcctttg caggagttcc gtacgcccgg tcactccgag taacgggttc tcggccccga
     2401 cttgaaaagc gcgttccaaa tcgcaggcct cgagcgactg aagccgggcg ctctcgacgc
     2461 gaaggggagt gcgccggtcc aacgccaaat caccgctcat gaaccagtcg aagctggcga
     2521 tcgcgaggcc ctccgagcgc cgcaatacta gccctgtctg tgactcccgg tatcgaaagc
     2581 tcgcgccggc gcccgcgtcg agtaacacag ttacgatcac gagatctgct tcggcccgca
     2641 agcgctcgag cggagaaagg ctaacgagcg ctgcatcgag ccgcgccact cgatctaccc
     2701 cggcggcgtt gaagtggcgg tagcggctgt gatacggcac gcgcgagagt tcgccgtagt
     2761 tttcgagcgt gaagcgcagg acctgtttcg cgagctccgg caggcgctcc ggtcgtaagg
     2821 caaagtgcgc gagctgattg gcttgtgcga gctggagtac ggcgtgagcc ctttcccgga
     2881 ttgcccgcgg gctcttcagg taagtgagct ctacttccgt taacgcgctc gcggaggcga
     2941 tcttttctag gctggcggct gcaccaacac ctccgccgag cgttcgcccc tcgtcattcg
     3001 gcaaggctgc gacccttggc tttttggagc tcatgcggat cgggcacggt gccttccgtg
     3061 aagtagccgg ccgctttttt cgcgtccatt tctacgcggg catcttccgg gatgaggtcg
     3121 tccgggatcg gaacccgctc gacgattcga atccccgaac gaaccagcgc gtcatgcttc
     3181 atattgctca tggatacgaa gcgatcgatc tttcgaatcc ccagccaatg cagcacatcc
     3241 ggcatgagct cctgaaagcg catgtcttgc acgccggcaa cgcactcggt gcgggcgaag
     3301 tattgttcgg ctcgatctcc gcctacctga cgcttgcgcg cgttgtaaac cagaaacttg
     3361 gtcacttcgc cgagcgcgcg gccctctttg cggttgtaga cgataacgcc agcaccgccg
     3421 gcttgcgcga cccgcacgca ctcctcgatt ccgtgcgtca agtaaggccg gcaagtgcag
     3481 atgtccgaac caaacacgtc ggagccgctg cattcgtcgt gaacccgcgc cgccaacggc
     3541 ttctcgggat ccgagatgga agccacatcc ccgaacaggt acacggtggt gccgccaatg
     3601 ggaggcagaa acacctcgag atcgctgcgc gtcacgagct cggggaacat accgcccgtg
     3661 tgttcgaaga tcgtactgcg caggtcggat tcgctgcacc cgaagcgtcg cgccaccccc
     3721 ggcaagtgcc acacgggttc tatcgccgcc ttcacgacca cgactccgcc gtcggccttg
     3781 agaatgcgtc cgtcggcacg caagcgcccg gcggccatgg cctcggtgag ctccggtaat
     3841 ttcagatgag ccttggtgat cgcaatgctc gggcgaatgt cgtacccctg ctcgaagtac
     3901 gaggcgaagg cgtcgctcac tagcgcgccg aacgggtcca gtgaaacgat gcgatccggt
     3961 tccgaccatt gagggaaggg gccgatcggg tgcgtaggcg cagtgttcgt gagatcggcc
     4021 ttgcgcagcg gatcgagcgc gcccgccgcc acggccaccg cccgatacac actataggaa
     4081 cccgaatgtg cgccaatcac gttgcgatgc gctcgattgg tgacggtgcc gatcacgggc
     4141 cctcgctcca tcgcctcacg cgccccccag tgaatggccg gcggacgcgg gccatgcggc
     4201 atggggtgcg tggctaggac gatgggtttg ggtcgcttct tcttggcttg ttctgtcacg
     4261 gtgtttccga attcagagcg caggaatggg atcgatcgac atcgtggtgt gtcggccgaa
     4321 gatcaggcaa atggcttcac gacatccaat tggcatcggt ctggagagcc catttggtcg
     4381 tgatcttctt cagattagtc cagaaatcga cggcttgggg cccggtgata tcgccgtgtc
     4441 caaacttgga ttgccgtgtt cctccgaagg aaaacggatc gcggggcacg ggcaccccca
     4501 cgttaacccc aaccataccg ctgagcgcgc gctcgcttac ataacgagcg acagcgccgc
     4561 tttgggtaaa aaccgaggtc gcgttgccgt aaggcgtggt ccgttctagc gccaacgctt
     4621 cgtcgagagt tcgcacttgg aggatcgtga ggaccgggcc gaacagctcc tccgtcgcac
     4681 acgccatccc aatcttggcg tccgcgatga cgctcggccc gagccaatgg cctcgctcga
     4741 agccagcggg gggtttcagc cgacgaccat ctagcagcag gcgtgcgcct tcttcttccg
     4801 cgcgagccag cgcacgcgcg agccgctctc gcgccgcggc gtcgatcagc gcgcccatct
     4861 cggtgcccat cttcaactcg gcggcacgag cgacgatggg ttcgatgaaa cggcctgctt
     4921 ccgccgccgc gcctacgcag acgagtacgc tgcccgccat gcacctttgc cctgcgcagc
     4981 cggtaaacga atcgactacc gcccggacgg tcagcgaggg atccgcatcg ggcatcacga
     5041 tcaagtgatt cttcgctccg cccaggcaca aggcgcgctt gccgtgctgc gcggcgcgcg
     5101 tgtaaacggc ccgcgccgcg cccgtggaac cgacgaagcc gaccgcgctg atctccgggt
     5161 gatcgagcag atgctccact gtctcccggc cgccgtggac gaccccgaac acgccagccg
     5221 gataacctgc ctcgtgcatc agctcggcca gtagcgcggc actcagaggg accttttccg
     5281 agggtttcag aatgaaagtg ttacctgccg tgagcgtgat gggcaacatc cagagcggca
     5341 ccatcatggg aaagttgaag ggggtgatgc cgagtgtaac cccgagtggt tctcgccgat
     5401 actcacagga cacaccgcgc gacacatcca acgccccacc gctctccatg ttgggccagc
     5461 tcagcgcgaa ctcgatcacc tccaagcctc gttcgatccc ggctcgagcc tcggcgggcg
     5521 tctttccgga ttcgtaggcg actagctcgg cgcagctttc cagccgagaa atcagcagct
     5581 cgcgcagccc aaatagaggc ttgcagcgtt ctcttacggg gagtgcgcgc cacgccggga
     5641 aggccgcgga ggcggcgtct acggctcgag cgacgtcctt tgcatccgaa atcgcgactc
     5701 gcgccacgtt atcccccgtg taggggctct ccaccggcag gctcgattgg aaaggctgaa
     5761 acgctcctga cacgaaatta ccgatatcgg actgacagga agggaaacgt atgttgttca
     5821 tcatcatgca gactgacccg acccgctcaa gcctactccg aactcgggcg ggcaccactt
     5881 cctggtcggc tcgagccgtt gaaggcgctt gctcacggtg cgggcaaaac cttcctggat
     5941 cccggcatgg gtaatcgctt cgtagctgat gacggcagca cgcagtgcgc gaatcgagta
     6001 ggcttactcg gcgtgtcgcc cctagtggta ggaattgcag gtggaaccgg aagcgggaaa
     6061 tccaccgtcg cgcgggagct ggtgcggtcc ttgcccgatc acgccgcggc tctgatccaa
     6121 cacgactggt actacagaga ccgtagccac ctgagtgaag cggagcgaga gagcatcaac
     6181 ttcgacgaac cggcggcgct ggataacgag ctgttggtcg aacacctact cgagctgaag
     6241 tgcgggcggg cagtggcttg tccgcagtac gacttcgtga cgcacacgcg cgcaaaggct
     6301 tctcaccatg tagagcctcg atccatcgtg ctcgtagagg gaattctcat cttcgccgtg
     6361 cgcgagttgc gacaggcttt cgatttgcgt ctgttcgtgg atacggacga cgacattcgt
     6421 ttgctccgtc gcgtgcagcg cgacatgcgt gagcgcggcc gcgacctgag ttcgatcgag
     6481 agccaatatc taaacacggt gcggccgatg caccaactgc acgtggcccc gacgcggcag
     6541 ttcgctcact tgatcattcc cgaggggggt gagaacgtcc aagcgctcga tgtgatcgtc
     6601 ggccgtttgc gctatctgtt ggctcgcgcg ggcagcgact agaacatccg tacttccaga
     6661 gtacgccgct ccggtaacgc cggagtgcgg ctccgttcgg gcgtcggatt tttgaccgat
     6721 cgagttccgg ctaggagcag gcgcgggcgg tgttataacg cgccccgtga cgaagtttag
     6781 ttggtatcgc agcacgcgca tcgcgtggat cgcaggtttt acgctcttca cgagcgcggg
     6841 ttgcatgcag cccgtcgggg ccggcgcgca gcaagctccg agtgctccga gtgctccgag
     6901 ttccgcgggt agcgccgcgc cgagcgcgag cgccgcgagc gcccccacgc cgactgcggc
     6961 ggcaccggtg gaagcgtcgg agcctgcgag cgcggaggcg accaccgttt ggcaggtgcc
     7021 ggtcactgat caggatcccg cacgggggcc caaagacgcg ctcgtgacga tcgtcgaatg
     7081 ggcggacttc cagtgtccgt attgccagcg ggtcgaagaa acgttgaagc gcgtcgagca
     7141 gacctacggt tcggacgtgc ggattgtctg gaaggacaac ccgcttggtt ttcacgaaca
     7201 cgcgatggcc gccgcgaagc ttgggcgagc tgtgttcgaa aagcagggca acgcgggctt
     7261 ttgggcggtc cacaaggcgc tgctcgagcg gcaggccgag ctttcgagcg ccgtcttgga
     7321 ggaactcgcg atcgggcaag ggctctcggc ttcacaatgg cgacgcctcg cgggcgactc
     7381 cgagtcggtg ttcatttcgg attcgatcgg cgcggggcaa gagctcggag tcacgggcac
     7441 gccggagttc ttcatcaacg gcgtgagctt gtcgggggct cagccgttcg aggagtttca
     7501 agcgcgaatc gacgcggagc tcgcgcgagc gaaggcgcgg gttgccgcgg gcgtgccacg
     7561 cgcggagctc tacgccacgt tgatgaaaga ggcgcaaccg gcacccccgc cacctcgcaa
     7621 agaggtggcg cccgcgcgca aagacagccc cgcgcgcggc cccgcgcggg ctaaagtgac
     7681 gctggaggtg ttctctgatt ttcaatgccc gttctgcaag actgggcggg acacgctgga
     7741 acgcctcgag aaaaagcatc ccgggcagct acgcgtggtg ttccggcact tgccattgcc
     7801 gtttcacgag cacgcgcgcc cagccgcaga ggcttccgag gaggtgcgca ttcagaaggg
     7861 aaacgccgcg ttttgggcct atcacgacaa gctctttgcg gcccagagcg agccgggtgg
     7921 gctctcggag gatcggctga tcgcgctggc ggtggagctc ggggtggatc gcaagaagct
     7981 cgaacgagcg ctcgcttcgg gcacccaccg cgccgcaatt cagtacgatc tcgacgccgc
     8041 cgcggcggcg gggatccgcg gcacaccgag ctttacgatc aatggctact tcgtgagtgg
     8101 tgcgcgcccg ctcgcgactt tcgaacgctt gattcgccgc gcggagcgag agaccgtgag
     8161 cgccgctggt acggcggcgc cgcgtccggc cagcgttgcg cccgcagcga ctgaaacacc
     8221 gccaaaggcc ccgtaaagca gagtgagtta gagccagacg aagagtggac ctgttgaacc
     8281 cactcttcct ccggatcgcg aaggtggctt acgtggcggg cacggccttc gtgtccgaca
     8341 acatggcgag ccaacgtcgc gcgctgccga tgatgatgaa actcacgccc acgagcaacg
     8401 cgctggtcac caacaaattg acgtagccc
//
LOCUS       DASTKU010000221         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_265666, whole genome shotgun sequence.
ACCESSION   DASTKU010000221 DASTKU010000000
VERSION     DASTKU010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265666"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..826)
                     /gene="eutC"
                     /locus_tag="VFQ61_08330"
     CDS             complement(38..826)
                     /gene="eutC"
                     /locus_tag="VFQ61_08330"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003971.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HET9954497.1"
                     /translation="MSELRKRDAWASLARHTAARIALGRAGGSLPTQAVLDFRLAHAL
                     ARDAVEMPFDADALLRELESAGVSGVKLGTRATNRRDFLLQPELGRQLDEDSRTRLRE
                     LFQGSGAGAHDLCVVVSDGLSALAAERQVLPLLRELWPQLSQLGVSLYPCFVVPFARV
                     KLADELGQLLQAKHSLILLGERPGLSSPDSLGAYLTFEPAPSRTDADRNCVSNIRPAG
                     LPVTQAAHTLLQLLRESRRLSVSGVALKVDSGPMILARPESQQA"
     gene            complement(835..2220)
                     /locus_tag="VFQ61_08335"
     CDS             complement(835..2220)
                     /locus_tag="VFQ61_08335"
                     /inference="COORDINATES: protein motif:HMM:NF011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HET9954498.1"
                     /translation="MSKPFSCQISGRHHAFGSLRELMAKATPKRSGDELAGLAAHNAE
                     ERVAAQICLSELPLTHFLQETVVPYETDEVTRLIVDSHDRQAFAPISRLTVGQLREWL
                     LDYATDGPMLSGLAGGLTPEMVAAVIKLMSNQDLILVAQKCRVVTRFRSTIGLPGTLA
                     VRLQPNHPADDPRGIAASILDGLLYGCGDAVIGINPATDSLERVTTLLQLLDELIARH
                     SIPTQSCVLAHVTTQLEALRRGAPLDLMFQSIAGTEAANRSFGISLSLLEEAHAATLE
                     LGRGTLGTDVMYFETGQGSALSAGAHHGVDQQTLEVRAYAVARRFKPLLVNTVVGFIG
                     PEYLYDGKQIIRAGLEDHCAGKLLGLPMGVDVCYTNHADADQDDMDTLLTLLGVAGCN
                     YIMGVPGADDIMLGYQSTSFHDAQYLRQVLGLRPAPEFEAWLQRSNIADARQRLLDVG
                     REHELLLQALA"
     gene            complement(2226..>2500)
                     /locus_tag="VFQ61_08340"
     CDS             complement(2226..>2500)
                     /locus_tag="VFQ61_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954499.1"
                     /translation="ARRRNGRQRERSAADDRALSGGFRGRRRGLGGEGAAERLRSRWS
                     WARFAHVVWRRQARDFFRKQAARTGLRLSGVINAMLRERVTQSQQE"
BASE COUNT          467 a          855 c          753 g          425 t
ORIGIN      
        1 acgcgcggcg ccgttcctgt cttggcaagc tgcaccgtca tgcttgctgc gactcgggac
       61 gcgccaagat catcggcccc gagtcgacct tcagagccac cccgctcacg ctgagccggc
      121 gggattctcg gagcaattga agcaacgtgt gcgccgcctg cgtgaccggc aatcccgcgg
      181 ggcgaatgtt cgagacgcag ttgcgatctg cgtccgtgcg agagggcgcg ggctcaaacg
      241 tgagataggc gcccaagcta tccggcgagc tcaggcctgg ccgttctccc aacaaaatca
      301 gtgagtgctt ggcttgcagc agttgcccca gctcatccgc caactttaca cgcgcaaacg
      361 gcaccacgaa gcagggataa agcgaaaccc cgagctggct cagctgcggc cagagctcgc
      421 gcagcagggg gaggacctgc cgttccgccg cgagcgcgga aagcccatcg gataccacca
      481 cgcacaggtc atgcgcgccc gcgccagagc cctggaacaa ctcacgcaag cgcgtgcggg
      541 aatcctcgtc gagctgccga ccgagctccg gttggagcaa gaaatcacgt cgattcgtgg
      601 cgcgcgtgcc gagcttcacg ccgctcacgc cggcgctctc gagctctcgt agcagtgcgt
      661 ccgcatcgaa cggcatttct acggcatccc gcgcgagcgc gtgcgccaat cgaaaatcca
      721 acaccgcctg ggttggcaag ctgccgccgg cgcgaccgag cgcgatccgc gcggcggtgt
      781 ggcgagccaa gctcgcccag gcatctctct tgcggagctc tgacatcgtc gacctcaggc
      841 gagcgcctgc aataatagct cgtgctcccg gccgacatcg agcaaccttt ggcgcgcgtc
      901 cgcgatgttc gaacgctgca accaggcctc gaactccgga gctggtcgca agccgagcac
      961 ttggcgcagg tactgcgcgt cgtgaaacga agtcgattgg tacccgagca tgatgtcgtc
     1021 cgctcccggt acccccatga tgtagttgca accggccact ccgagcagcg tcaacagcgt
     1081 atccatgtcg tcttgatcgg cgtcggcgtg gttggtgtaa caaacgtcca cccccatcgg
     1141 taagcccagc aatttgcccg cgcaatggtc ctcgaggccg gcgcggatga tctgcttccc
     1201 atcgtacaag tactcgggac cgatgaagcc cactaccgta ttgacgagca ggggcttgaa
     1261 gcgccgcgcc accgcatacg cccgcacctc cagcgtttgc tgatctacgc cgtgatgcgc
     1321 cccggcggag agggccgagc cttggccggt ctcgaagtac atcacatccg tgccgagcgt
     1381 gccgcgcccg agctcgagcg ttgccgcgtg ggcctcctcc agcagagaca aagagatgcc
     1441 gaaactccta ttcgccgcct cagtcccggc gattgactga aacatcaaat ccagcggcgc
     1501 gccccggcgg agtgcctcga gctgagtcgt gacgtgggcg agaacgcacg actgagtggg
     1561 gatcgagtgt cgcgcgatca gctcgtccaa cagctgcaga agcgtagtta cccgctccaa
     1621 actgtcagtt gcgggattga tgccaatcac cgcgtctccg cagccgtaca acagaccatc
     1681 caagatcgat gcggcgatcc cgcgcggatc atccgccggg tggttcggtt ggagacgcac
     1741 cgcgagcgtc ccgggcagcc cgatcgtcga acgaaagcgc gtcaccacgc ggcacttctg
     1801 ggccaccaga atcaggtctt gattgctcat cagcttgatc acggccgcca ccatttccgg
     1861 cgtcaatccg ccggccaggc cactcagcat gggtccgtcg gtcgcataat ccagcaacca
     1921 ctcgcgaagc tgacccacgg tcagccgtga tatcggcgcg aacgcctggc gatcgtgcga
     1981 atcgacgatc agtcgagtca cctcgtctgt ctcgtacggc actaccgttt cctggaggaa
     2041 gtgagtgagc ggtagctcgg acagacatat ttgcgcggcc acgcgctcct ccgcgttgtg
     2101 cgcggcgagc cccgccagct catccccgga gcgcttggga gtggcttttg ccatcaactc
     2161 tcgaagtgag ccgaacgcgt gatgccgccc ggatatctga caggaaaacg gcttgctcat
     2221 ggcgactact cctgctgcga ctgggtcact cgctcgcgca gcatagcgtt aatcacgccg
     2281 ctcaggcgta ggcccgttcg tgcggcctgt ttcctgaaga agtctctggc ttgccgccgc
     2341 caaactacgt gcgcgaagcg cgcccaagac cagcgagagc gcagcctctc cgccgcccct
     2401 tcacccccca aaccgcgccg gcgccctcgg aacccacccg atagggctcg gtcgtcagcg
     2461 gccgatctct cgcgttgtcg gccgtttcgt cggcgcgcta
//
LOCUS       DASTKU010000222         5088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_935656, whole genome shotgun sequence.
ACCESSION   DASTKU010000222 DASTKU010000000
VERSION     DASTKU010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935656"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFQ61_08345"
     CDS             <1..352
                     /locus_tag="VFQ61_08345"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HET9954500.1"
                     /translation="GHTLEVRDLLSWPWDEASLSIFLTPLPVPEWFNRAAPRVKSGDV
                     VPERLERHEAIALLLAEPLLIRRPLMALPNGDRMVGFETDRVQAWIGLGGDVPTGRSL
                     ESCAAETMKSTRPR"
     gene            complement(393..1286)
                     /locus_tag="VFQ61_08350"
     CDS             complement(393..1286)
                     /locus_tag="VFQ61_08350"
                     /inference="COORDINATES: protein motif:HMM:NF018431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9954501.1"
                     /translation="MSELAQIAGRHAPRDGMLPTAVPELHVVRATQRSQPVHAVQRPS
                     LCFLVQGAKEVSVAGTTYRYTAGEHLVSTVDLPITGEIVEASPRNPYYCLVISLEPAL
                     VQRVLDDGKFEVPDGPGPGIFVGRPEPRLADAALRLARCLEDERDGRVLAPSILREIV
                     YRLLCGPYGGTVRDLGTSGSRTQRIARAIERLKNSFAEPLKMAELAALAGMSPSSFHE
                     HFKRITTLSPLQYQKVLRLQEARRLLEQGFGAADVAFRVGYQSPSQFSREYARCFGAS
                     PRSDARPRGASEPRRGARVLR"
     gene            1377..2213
                     /locus_tag="VFQ61_08355"
     CDS             1377..2213
                     /locus_tag="VFQ61_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9954502.1"
                     /translation="MQNDVWLITGGSKGIGLELAKKLLRSGRRVAVTTRAVEAARDAL
                     GADVCVVPVDLADEASVGRGVDSVIQRFGRIDVVVNNAGYGQMGAVEEVSDAEARRNF
                     DVNVFGVLNVLRRTLPTLRQQRAGHVFNIGSVGGFTGGFAGWGIYCATKFALSGLTEG
                     LHRDLAPLGVKVTLVYPGYFRTEFLSSDSVHRPRTRIADYTSALESETLHLGSIHQNQ
                     PGDPARLAEALIATREMAEPPLHLFLGSDAFGMARDKLAAVERELSAHHELTFSTDFP
                     KA"
     gene            2485..>5088
                     /locus_tag="VFQ61_08360"
     CDS             2485..>5088
                     /locus_tag="VFQ61_08360"
                     /inference="COORDINATES: protein motif:HMM:NF041916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6230/SCO0854 family RING domain-containing
                     protein"
                     /protein_id="HET9954503.1"
                     /translation="MQKDRRRAAREVLLKRRGLVFVDSSAGPQSERLLKALEVELSTL
                     GYALSTRLRAALFQSSLEDLSATRAWVWAVLAAELGAAHAHVPLFRDFPDGVPDDTHK
                     LWMRRVLSHFLQASGQPCLFCQRLGTTHVLSPCLHVVCDQCFDVNQYSGCPVCGHKLD
                     PKSPFLQRSSPQRQGLPREAVRFKLLDLGRDLDRECSAYFVRLCERKQALSPTDKADL
                     LALVTDYGSLVLDWVPASIPVRENVALVFGTLLQVCPPSDVMAAAKGYVATATDVLRL
                     VAAYSGADPSLQAQTTYRSIVRIEGPARWWGKIAAALGAQQPSARTRPVFVPIQLKRF
                     RVAKLSRPLRRELLGLLDSFDAEHLAEDMLRHRSYWVWAGQFLHPHEYADRYPNAARA
                     FAIARKKSPDGTRAPAFQGYYARLEAAISAGDSAALLELLASRPGELARRFDHALRVA
                     GEDAAAVEKLLARFLRTAGAFSTPVLLTLRSHLPSRTHPASVRIFWPKGGVTKGVSSP
                     DRRKVLPAPVLGRAVFQIEEELLKRFAAKGRVETCIIDTALREVIAPFNERTASPAAV
                     ALPRGSRIAVPRSKVVRMFIHWCEPERGGHTTDVDLSVGFYSRDWKHVGVCSYYNLKL
                     KGQSGSEVALSSGDYTSAPYPDGSSEFIDLYRERALAEGMRYAVMVVNAYSGMPFSLL
                     ERAFAGLMLRDDVQGMHFDPRTVELKFSLQGANGVFLPLVFDLEAGTLHWLDVYSKGQ
                     LELNNVENSTSAITKICPEMIDYFSSGVRTTMFDLALLHGAARAGRVLVRGREWRCFE
                     RTADETSGQFLQRIRTELDAETKNFPAPVEPVVPVPTGPVLAVLYRGDLPLPENSAVY
                     ALFREGMIPNLS"
BASE COUNT          888 a         1594 c         1641 g          965 t
ORIGIN      
        1 ggggcacacg ctcgaagtcc gtgacctcct gagctggcct tgggacgaag catcgttatc
       61 gatctttctg acgccgttgc cggttcccga gtggttcaat cgcgctgcgc cgcgggtcaa
      121 atcgggcgac gtcgtgccgg aacgcttgga gcggcatgag gcgatcgcgc tgctcttggc
      181 ggagccgctt ctgatccgga ggcccctgat ggcgttgccg aatggggacc gaatggtggg
      241 attcgagacc gatcgagtcc aggcctggat cggacttggc ggcgatgttc ccactggacg
      301 ttcgctcgag agctgtgcgg cagagacgat gaagtcgacg cgacctcgat aggttcaggg
      361 cggcacccgc cgagtcagcg aagcgcctcc gttcaccgaa gcacccgggc cccacggcgg
      421 ggttcagacg cgccgcgcgg tcgagcgtca ctccggggcg acgcgccgaa gcagcgcgcg
      481 tattcgcggc tgaactgcga ggggctctga tacccaacgc gaaaggccac atccgccgct
      541 ccgaaccctt gttcgagcaa acgacgcgcc tcctgtaggc gcaggacttt ctgatattgg
      601 agagggctca gcgtggtgat gcgcttgaag tgctcatgaa aggacgacgg gctcatgccc
      661 gctagcgccg ccagttcagc catcttcagc ggctccgcga aggagttttt caggcgctcg
      721 atggcgcgcg cgatacgttg agtgcggctg ccgctcgtac ccaaatctcg cacggtgcca
      781 ccatacggac cacataacag gcgatatacg atctcgcgca ggatactggg cgccaaaacc
      841 cggccgtcgc gctcgtcttc caggcaacgc gccaggcgca gcgccgcgtc cgcgagccga
      901 ggctccgggc gacccacgaa gatgccggga cccgggccgt cgggcacctc gaatttgcca
      961 tcatcgagta cccgctgcac cagcgccggc tctagcgaaa tgacgaggca gtagtaggga
     1021 ttgcgcggtg aagcttcgac gatctcaccc gtgatcggta aatctacggt ggagacgagg
     1081 tgctcgcccg cggtgtaccg ataggtggtg cccgcaacgc tgacctcctt tgcgccttga
     1141 acgaggaagc agagcgaggg gcgttgcacc gcgtgcaccg gctggctgcg ctgcgtcgcg
     1201 cgcaccacgt ggagctcggg caccgcggtc ggtagcatgc cgtcgcgcgg ggcgtgacga
     1261 ccagcgatct gggcgagttc gctcatgctc tgaagatgtg cctactcgga ggattaggca
     1321 agttctacgt aagtttggtc taccctggtt cacaccggac gccttaaatc gaagccatgc
     1381 aaaacgacgt ttggttgatc acgggtggct cgaaaggcat tggtttagag ctcgcgaaga
     1441 agctcctgcg gagcgggcgg cgcgtggcgg tcacgactcg cgcggtagag gcggcgcggg
     1501 atgcgctcgg agcggatgtc tgcgtcgtcc ccgttgacct ggcggatgag gcaagcgtgg
     1561 gacgcggcgt cgattcggtg attcagcgct ttgggcgcat cgatgtcgtc gtgaacaacg
     1621 cgggttacgg tcagatgggt gcggtggaag aggtgtcgga cgcagaagcg cggcggaact
     1681 tcgacgtcaa cgtgttcggc gtgctgaacg tgctgcgcag aacgttgcca acgctgcgcc
     1741 aacagcgcgc gggacacgta ttcaacatcg gctcagtcgg cggcttcacg ggtggcttcg
     1801 cgggttgggg catctattgc gccaccaagt ttgcgctctc ggggctcacg gagggcctcc
     1861 atcgagactt ggcgccgctc ggggtgaagg tgacgctggt gtatcccggc tacttccgca
     1921 ccgagttcct atcctctgac tcggtccatc gcccgcggac gcgcatcgcg gactacacat
     1981 cggcgctcga gtcggagacg ttgcacctcg gctcgattca ccaaaaccag ccgggtgacc
     2041 cggctcgcct cgccgaggcg ctcatcgcca cgcgtgaaat ggccgagccg ccgctgcact
     2101 tgtttctcgg cagcgacgct ttcggcatgg cgcgggacaa actggcggcc gtcgaacgcg
     2161 agctcagcgc gcaccatgag ctcacgttct ctacggactt ccccaaggcg tgaggcggat
     2221 cgagttctgg ccagacgggg acgtttcggg tatggtccgg cggaaggctt agtccgggct
     2281 tccttctaac tttggaacca gctcggacga tttccttcac aatttcttgg ctggcccgct
     2341 cgacacgaag tctccctcat aggctaagac tctctgctca ttgactctgt gcggagatac
     2401 tctcggccta gagactccgt gcagagcgaa gcagccggcg ggctagcgta ggttttggta
     2461 gaccggggtt gtatggtgga aggcatgcaa aaagaccgtc gcagggcggc gcgggaggtt
     2521 ttgctcaagc gccggggtct agtcttcgtc gattcatcgg cggggccgca atccgaacgc
     2581 ctcttgaagg ctctcgaggt agagctttcg actctcggat atgctctgtc cacgcggctt
     2641 cgcgcggcgc tcttccaaag ctcactcgag gatttgtcgg cgacccgtgc gtgggtatgg
     2701 gccgtgctcg ccgccgagct tggtgcggcc cacgcccacg tgccactgtt tcgcgacttc
     2761 ccagacggag tgcctgacga cacgcacaag ctttggatga ggcgcgtgct cagtcacttc
     2821 ctgcaagcca gcggtcaacc ctgcctgttt tgccaaaggc tcggcaccac ccacgtgctc
     2881 tccccttgtc ttcacgtggt ctgcgatcag tgcttcgatg tgaaccaata ctccggctgc
     2941 cccgtatgcg gtcacaagct cgatccgaag tcaccgtttc tgcagcgatc gagcccccaa
     3001 cggcagggtt taccgagaga agccgttcgc ttcaaattac tcgacctggg tcgagatctc
     3061 gatcgtgagt gctccgcgta tttcgtgcgt ctctgcgaac gcaagcaggc gctctcaccc
     3121 acggacaagg ctgacttact ggccctcgtc accgactatg gctcgctcgt gctcgattgg
     3181 gtgccagcct caatccccgt acgggaaaac gttgcgctcg tgtttggcac cctactccaa
     3241 gtgtgcccgc cgtcggacgt gatggcagcg gccaagggct acgtagcgac ggccaccgac
     3301 gtactgcgac tggtggccgc ctacagcggc gcggatccgt cccttcaagc gcaaacgacc
     3361 taccgctcga tcgttcggat cgagggcccc gcacgttggt ggggcaagat cgcggccgcc
     3421 ctcggagcgc agcaaccctc cgcccgcacc cgccccgtgt ttgtgccaat tcagctcaag
     3481 cggttccggg tggccaagct gagtcgcccg ctgcgccgcg agctgctcgg cttgctcgat
     3541 agcttcgatg ccgagcacct ggcggaagac atgctcagac accgctcgta ttgggtttgg
     3601 gcagggcagt tccttcaccc gcacgaatat gcggaccgct accccaacgc cgcgcgggcc
     3661 tttgcaatcg cgcgcaagaa gagccccgac ggcacgcgag cgcccgcctt ccagggctat
     3721 tacgccaggt tggaggcggc tatctctgcc ggggattcgg cggcgctgct cgaattgctc
     3781 gcgagccgac cgggtgagct ggctcgccgc ttcgaccacg cgctccgggt cgcgggtgag
     3841 gacgcggccg cggtcgaaaa gctgctcgct cgttttctgc gcacggctgg agcgttctcg
     3901 acgccggtgc tgctgacgct tcggagtcat ctaccctcgc gcacgcatcc agccagcgta
     3961 cgcatctttt ggcccaaggg cggtgtgacc aagggagtgt cgagcccgga ccgacgcaag
     4021 gtgttgcccg ctcccgtact cggacgagcg gtattccaga tcgaggagga gctgctgaag
     4081 cgtttcgcag ccaagggcag agtcgagacg tgtatcatcg acaccgcgct tcgcgaggtc
     4141 atcgcgccgt tcaacgagcg caccgccagc cccgcggcgg tagccctgcc gcgcggctca
     4201 cgcattgccg taccccgcag caaagtcgtg cgcatgttca ttcactggtg tgaacccgaa
     4261 cgcggcgggc acaccaccga cgtggacttg tcggtgggct tctacagtcg cgactggaag
     4321 cacgttggcg tgtgttcgta ttacaatctc aagctcaagg gccaatcggg ctcggaggtg
     4381 gcgctcagct caggtgatta cacgagcgca ccctaccccg acggctccag cgagttcatc
     4441 gacttgtacc gtgagcgcgc gctcgccgag ggcatgcgat atgcggtgat ggtcgtgaac
     4501 gcatactccg gaatgccttt cagcttgctc gaacgggcgt tcgcgggcct gatgctacgg
     4561 gacgacgtgc agggcatgca tttcgatcca cgcacggtgg agctgaagtt ttcactacaa
     4621 ggagccaacg gcgtattcct gccgttggtc ttcgacctcg aagccggaac gctgcattgg
     4681 ctggacgtgt actcgaaggg gcagctcgag ctgaacaacg tagaaaactc gacctccgcg
     4741 atcaccaaaa tctgtcccga gatgattgac tatttctcga gcggagtgcg aaccacgatg
     4801 ttcgatttgg cgctgctcca cggcgccgca cgcgcggggc gcgtcctcgt tcgggggcgt
     4861 gaatggcggt gcttcgagcg cactgcagac gaaacgtccg gccaatttct gcaacgcatc
     4921 aggaccgagc tggacgccga gaccaagaat ttccctgcgc ccgtcgagcc agtagtgcca
     4981 gtgcccacgg ggccagtgct cgcggtcctc taccgcggcg acttacccct tcccgaaaac
     5041 agcgcggtgt atgccttgtt tcgtgagggg atgattccca acctgtcc
//
LOCUS       DASTKU010000223         4130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_935689, whole genome shotgun sequence.
ACCESSION   DASTKU010000223 DASTKU010000000
VERSION     DASTKU010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935689"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VFQ61_08365"
     CDS             <1..548
                     /locus_tag="VFQ61_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954504.1"
                     /translation="GTRYDLDGTGVPVSAALDLEYTFGSQNVGTPLIGVPRGGTDSIA
                     VPNVFEWDDSLTARVGLEYRVGRYLTRAARVATDSANLPDAGWAFRVGYVLDTRATNP
                     AYPTAFGTPPAATNVLTAGAGYRSGPFKANLAYAYRFGSAEVSAGDLTAPDRKKCSFC
                     GDRGEYGMYLSGLYVDVGYQF"
     gene            complement(578..1018)
                     /locus_tag="VFQ61_08370"
     CDS             complement(578..1018)
                     /locus_tag="VFQ61_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954505.1"
                     /translation="MPKPASTLLFETIPEAGLLISYQTTATPRDDEWEAYLTACKELL
                     ASHPKPCFLVVTHGGHPSRQQQARVKAELGYRTSPVAIISKATIARFVASIMALMNPA
                     IQCFAPEQRDQAFSHLHLDAAQRKLVLASLDRMTKKLGESSAAA"
     gene            complement(1144..1638)
                     /locus_tag="VFQ61_08375"
     CDS             complement(1144..1638)
                     /locus_tag="VFQ61_08375"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HET9954506.1"
                     /translation="MSDLVAFTDGGCRGNPGIGAWAFVLVDIKTRKALERAGGDALTT
                     NNRMELSALLEALLAVKNPGASILVVSDSKYTIDCCSKWMPGWKKNGWKRKGDPLKNL
                     DLLQSLDVQLARHQVKFAWVAGHSGHPGNEHVDRLGNEAMDRLAVGGDPAHERRFEWT
                     EALR"
     gene            1804..3792
                     /locus_tag="VFQ61_08380"
     CDS             1804..3792
                     /locus_tag="VFQ61_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9954507.1"
                     /translation="MTQARHDPPESLLPRGRAAWRSVRAALSSVGAYFTEQNPNFFAA
                     LTPALLVTALLFVRSPTSNYIFDEQEALLANPYVNAQGLKYWEAVKRDFWGLLPDRSI
                     GSYRPIPNYIWRALWHISKQPFLHHWVNVIGHAVNAALVASFCFALTQRRRVSWLAGA
                     CFAASALLTEAVTGVVGIADVLGGLGVLLALHALRLPLWATPGAVFLGLSFGLFSKES
                     AIVGVPLLTLAALISAWALKDRRPLGPVRALVVLLSAVAALVVYTEFRRRFFPVALPS
                     DLAQGLPEGTPVGRRALHAFLRWFQQPQLPNDAINNPLVLADTPHRIAGALRVFASGI
                     GQVVWPWNLSGDYSFPQEPVPARLVFVGSVVGGAFLLGLPLFAFGSWLVAIDRHRARQ
                     QAPAPSPEPSGQDSFGPPRGLELPPVTRKDGRTALVLALGALWLPIAYFPHSNIPVLL
                     PTVRAERFWYLPMVGAALLLAVLLDRLLEIRWRALGIRLVLAFFAVQIVQARAHALDY
                     ADDLAFWDATRRAVPNSAKAHLNYSVMVGARGRLEDRLQANRRALDLAPNWPMAHVYY
                     GDTLCRMQKPDAAFPYYKRGFELAPNDPNLIALGLQCLWDQKGVESRKAELLELAGHH
                     TGSWLALLATELVYYGPEHNGIQPKYRPRSYNEGPRSE"
BASE COUNT          674 a         1455 c         1222 g          779 t
ORIGIN      
        1 tgggcacacg ctatgacctc gacggtaccg gcgtgccggt gagcgcggcc ctagatctgg
       61 aatatacgtt tggtagccag aacgtcggca ctccgctgat tggagtgccg cgcggcggca
      121 ccgattctat cgccgtcccg aacgtgttcg agtgggacga ctcactgacg gcccgggtcg
      181 gtctcgaata tcgagtcggt cggtatctga cgcgggcagc gcgcgttgct acggactccg
      241 caaacctgcc ggatgcgggt tgggccttcc gcgtgggtta cgtgctcgac acgcgagcca
      301 ccaatcccgc gtatccaacg gcgttcggca cgccgccagc ggcgaccaac gtgctgacgg
      361 ccggcgcagg ttatcgctcg gggccgttca aggccaatct cgcctatgcg taccgcttcg
      421 gtagcgccga ggtcagcgcc ggtgatctga ccgcgccgga ccgcaagaag tgtagctttt
      481 gcggcgaccg cggcgaatac ggaatgtacc tgtcggggtt gtacgtcgac gtcggctacc
      541 agttctgaga gttcagttcg accccacccg tcggggctca agcagcggcc gaactctcgc
      601 cgagcttctt cgtcatgcga tcgagcgagg ccaagaccag cttgcgctgt gccgcgtcca
      661 aatgcaggtg gctaaacgct tgatcacgct gttccggcgc aaaacactga atggccgggt
      721 tcatcagcgc catgatcgat gcgacgaagc gcgcaatcgt cgccttcgaa atgatagcca
      781 cgggtgacgt tcgatagccg agctccgcct tcacacgcgc ctgttgctgg cgcgacggat
      841 gcccgccgtg cgtcaccacc agaaaacacg gcttcggatg ggaagccagc agctccttgc
      901 aagcggtcag gtatgcctcc cactcatcgt cacgcggggt cgcggtggtt tggtacgaga
      961 tgaggagccc tgcttccggt atcgtctcga acaacagagt agaagcaggt ttgggcatga
     1021 gcgcttgcag ccgagaacgg acatccgtct gaaggggttg agtcaaatcc accacaccct
     1081 cgaaaacttg gagcagacac cctcacatca gagggggtct tgcgagccgc gcgttggcgc
     1141 aggtcagcgc aacgcttcgg tccactcgaa gcggcgctcg tgtgcagggt ctccgcccac
     1201 cgccaagcga tccatggcct cgttgcccag acggtcgacg tgctcgttcc cgggatgacc
     1261 cgagtgacct gccacccagg caaacttcac ctggtggcgc gcgagctgca catcgaggct
     1321 ctgcagtaga tccaagttct tgagcggatc gcccttgcgt ttccagccgt tcttcttcca
     1381 gccgggcatc cacttactgc agcagtcgat ggtgtacttg ctgtcactca ctaccaaaat
     1441 cgaggcgccc ggattcttga ccgcgagcag cgcctcgagc agcgcactca actccatccg
     1501 gttattggtc gtcagcgcgt ctccccccgc ccgctcgagc gccttgcgcg ttttgatgtc
     1561 caccagcacg aatgcccagg cgccaatccc ggggtttccg cgacagccac cgtccgtaaa
     1621 agcaaccaga tccgacacgc gggcgcagcc tcgcagagcc gggcagctcg cgcaaccccg
     1681 actgaccgga gggctcagag caccggcgca gtaatgatac gctggagacc gcgcggaccg
     1741 accgagccgc ttctcccttc cgtttcccgg cccgacgcgc tacccagtcg cacggttcgc
     1801 gttttgactc aggctcgcca cgaccccccc gaatccctgc tcccgcgcgg gcgcgccgcg
     1861 tggcgaagcg tgcgggccgc gctgtcttcg gtaggggcgt acttcaccga gcagaacccc
     1921 aacttcttcg cggcgctcac gcccgcgttg ctggttacgg cgctgctctt cgtgcgcagt
     1981 cccacctcca attacatttt cgacgagcag gaagccctgc tcgccaaccc ctacgtgaac
     2041 gcccaggggt tgaagtattg ggaggccgtg aagcgcgatt tttgggggtt actcccggat
     2101 cgcagcatcg gttcgtatcg accgattccg aactacatct ggcgcgcgct ctggcacatc
     2161 agcaagcaac cctttctgca tcattgggtc aacgtcatcg ggcacgccgt caacgccgcc
     2221 ctcgtggcga gcttctgctt tgcgctgacc cagcgccgca gagtgtcgtg gctggcgggg
     2281 gcctgcttcg cagcgagcgc gctcttgacc gaagccgtga cgggcgtcgt cggcattgcg
     2341 gacgtgctgg gcggcttggg cgtgctgctc gccttgcacg ctctgcgcct gccgctttgg
     2401 gccaccccgg gtgcggtatt tctcggccta tccttcgggc tattctccaa agagagcgcc
     2461 atcgtgggcg tgccattgct cacgctcgcg gcgctgatct ccgcatgggc cttgaaggat
     2521 cggcgcccgc tcgggcccgt gcgagcgctc gtggtgctgc tctcggccgt cgcggcgctg
     2581 gtggtctaca ccgagttccg gcggcgcttc ttccccgtcg cgttgccgag cgatcttgcc
     2641 caaggcctgc ctgagggcac gcccgttgga cgacgcgcgc tccacgcttt tctgcgctgg
     2701 ttccaacaac ctcaactccc gaacgacgcc atcaataatc ccctcgtgct ggccgacacg
     2761 cctcatcgca tcgctggggc tttgcgcgtc ttcgcgagcg gcatcggaca ggtggtgtgg
     2821 ccgtggaacc tgagcgggga ctactcgttt ccgcaggagc cggtgcccgc gcgtttggtg
     2881 ttcgtcggca gcgtcgtggg gggtgccttc ctcctaggct taccgctgtt tgccttcggc
     2941 tcgtggctcg tcgcgatcga tcggcatcgt gctcggcagc aagccccagc cccctcgccc
     3001 gaaccctccg gccaagattc attcggaccg ccgcgcgggc tcgaactgcc ccccgtaacg
     3061 cgcaaagacg gtcgcaccgc cctcgtgctg gccctcggcg cgttgtggct acccatcgct
     3121 tacttcccgc attccaatat cccggtgttg ctacccaccg tgcgtgcgga gcgcttctgg
     3181 tacctgccca tggtgggcgc cgcgctcttg ctcgcggtgc tactcgatcg gctgctcgag
     3241 atccgttggc gagcgctcgg catacggctc gtactcgcgt tttttgcggt tcaaatcgta
     3301 caagcccgcg cccacgccct cgactacgcc gacgacctcg ccttctggga cgccactcgg
     3361 cgagctgtcc ccaacagcgc caaagcccat ctcaactact ccgtcatggt cggcgcgcgt
     3421 ggccgcctcg aagaccgcct tcaagccaac cgacgcgcgc tggacctcgc ccccaactgg
     3481 ccgatggctc acgtgtacta cggcgacacc ctctgccgca tgcaaaagcc cgacgccgcc
     3541 tttccatatt acaagcgcgg cttcgagctc gcgcccaacg accccaattt gattgccctc
     3601 ggcctacagt gcttgtggga tcaaaagggc gtcgagagcc gcaaagccga actcctcgag
     3661 ctcgctggcc accacacggg ctcgtggctc gccctgctcg ccactgaact cgtctactac
     3721 ggccccgagc acaacggcat ccaacccaag taccgcccgc gttcttacaa cgaaggcccg
     3781 cgctccgagt aaccaaactt cattgtctcg ccatctcgtt ggccttggcc gcaccatcac
     3841 gagcgcgact gctcgtcgcg cgacgtccct ggcgctgccc catctctccc atcccgtccc
     3901 aacaccgcgc aggcccccaa atccccacga ccgctcaatc cggcgcctta tcccactcgg
     3961 gaccccaact tgattcggcg atggtcccgg tccccagcgc tcgtcacgtt gcaccgccgg
     4021 gagacgcacc gatgacgacg accagcccgt gatcgacttg attcggtgat ggtcccggtc
     4081 gccagcgctc gtcacgccgc actgccggga gacgcaccga tgacgacgac
//
LOCUS       DASTKU010000224         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1828925, whole genome shotgun sequence.
ACCESSION   DASTKU010000224 DASTKU010000000
VERSION     DASTKU010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828925"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFQ61_08385"
     CDS             complement(<1..312)
                     /locus_tag="VFQ61_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET9954508.1"
                     /translation="MSSSCPIASRALEGVLAPLERASGLPREAYVDEAVFAFELERVF
                     RPSWLCIGRESELRLPGDFLVAQVADESVLAVRGTDLKLRAFYNVCRHRGLELVREER
                     GR"
     gene            509..1270
                     /locus_tag="VFQ61_08390"
     CDS             509..1270
                     /locus_tag="VFQ61_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954509.1"
                     /translation="MAKSSVATHSTRWSLIVRAQGAGDTRVALGELICHYEKFIVWLI
                     QHHRHPPDTSAEELKQEFLEGVLRRNDIAKLDRQRGSFRGWLGLAVRRFLFNEWNKWK
                     AASAGRMGTIPMGLEVVDGQIAPDDASDREFARHVVLRALALHRDEQRDKARFDTLAR
                     FLPGPQMDLTELAPVAEMLGLTPTALAKSICVLRARFRGLLREAVADLIDLDSRSMPN
                     VDEHPLAPAPEVTPQLAQSKLIDEELRDLRRHFWK"
     gene            1261..>2247
                     /locus_tag="VFQ61_08395"
     CDS             1261..>2247
                     /locus_tag="VFQ61_08395"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9954510.1"
                     /translation="MEVNQCGTCHAELLSTQWGELCGACALQGGIEHESELIGDYERY
                     ELLGEGAMGAVYLARHIHSDEVVALKLAKAELLQRAGGGALFRQQAKLESALKHPNIV
                     TVRAGGNHEGTPYVVMPLMEGGTLAESANAQRFTVPQARLELLLKIARAVQFAHERGV
                     LHCDIKPENILFDAAGEPYVSDFGLARSLDAAPAAVSVEGGTRGWMSPEQARRDSLTT
                     ASDVFALGVLLHWLATGELPFGRGDDFEQRVQNDPPPRLRPWSPELAWGLEAIAARAL
                     AKAPADRYDSAAALVEDLERLRAERCVRGQPVPFWARTWHWAQRHPGARNVIF"
BASE COUNT          425 a          707 c          714 g          401 t
ORIGIN      
        1 ccgcccgcgc tcctctctca ccaattcgag gccgcggtgc cgacacacgt tataaaaggc
       61 gcgcagcttc aggtccgtac ctcgaaccgc gagcaccgat tcatccgcca cctgcgccac
      121 taggaagtcc cccggcagac gcagctcact ttcgcgaccg atgcacagcc agctcggccg
      181 gaacactcgt tcgagctcga acgcgaacac cgcctcgtcc acgtaggcct cacggggtag
      241 cccactcgct cgctcgagcg gcgctagcac gccctcgagg gctcgtgagg cgatggggca
      301 ggaagacgac accgcgggaa tattaccgat ctgcaggcaa acgagtattc gcgttttgat
      361 actgagaggc cgcgtgagag accggctaaa acctccccgc cccgcttcgg ggtcgtttaa
      421 cctcgactca ccggccaaat ccgcttttta gaggtcggct ggtaatgccg ttcccaaagc
      481 gcttaatcaa aggggggaaa ggattagcat ggccaaatca agtgtcgcga cccactcgac
      541 tcgctggtca ctgatcgtcc gcgcgcaggg ggctggcgat acccgcgtcg cgctcggaga
      601 gctgatttgc cattacgaga agtttatcgt gtggctcatt cagcaccata gacatccgcc
      661 cgacaccagc gcggaggagc tgaagcaaga gtttctagaa ggcgtgctgc ggcgcaacga
      721 catcgccaag ctcgatcgcc agcgcggctc gtttcgaggc tggctggggc tcgctgtgcg
      781 ccgcttcttg ttcaacgagt ggaataagtg gaaggcggcg agcgccggac gcatgggcac
      841 gatccctatg gggctcgaag tggtcgacgg gcagatcgcc ccggatgatg cgagcgatcg
      901 cgagttcgcg cgtcacgtag tactgcgcgc gctcgcgctt catcgcgacg aacaacgcga
      961 caaagcgcgc ttcgacaccc tcgcccgctt cttaccaggg ccgcagatgg acttgaccga
     1021 gctggcaccc gtcgcggaga tgctgggctt gactccgacc gcgctcgcta agtcgatttg
     1081 cgtgctacgc gcgcggtttc gcggactgtt acgggaagcg gtcgccgatc taatcgactt
     1141 ggactcccga tcgatgccga acgtggacga gcatccgctt gcaccggctc cggaagtcac
     1201 cccacagctc gctcaatcca agctcatcga tgaggaactg cgtgacttga ggaggcattt
     1261 ttggaagtaa atcagtgcgg cacctgccac gccgagctgc tgagcaccca atggggagag
     1321 ctgtgcggag cgtgcgctct ccagggcggc attgagcacg agtccgagct cattggcgac
     1381 tacgagcgtt acgagttgct cggcgagggt gccatgggcg cggtctatct ggcgcgccac
     1441 atccactccg acgaagtggt tgctctcaag ctcgcgaagg ccgagctgct ccagagagcc
     1501 ggcggcggtg cgctgtttcg gcaacaagcc aaactcgaga gcgcactcaa gcacccgaac
     1561 atcgtgactg tgcgcgcagg cggcaatcat gagggaacac cgtatgtagt gatgccgttg
     1621 atggaagggg gcacgctcgc cgaaagcgcg aacgcacagc gatttaccgt gccccaagca
     1681 cgcctggagc tgctgctgaa gatcgcccgg gcggtgcagt ttgcgcatga acgcggcgtg
     1741 ttgcactgcg acatcaagcc agaaaatatt ttgttcgacg cggcgggtga accctacgtg
     1801 agcgacttcg ggctcgcgcg tagcctcgac gcggctccgg ccgcggtttc ggtcgagggg
     1861 ggcacacgcg gctggatgtc cccggaacaa gctcgccgcg actcactcac caccgcttcc
     1921 gacgtgttcg cgcttggagt gttattgcat tggctcgcta caggggagct cccgttcggg
     1981 cgaggtgacg atttcgaaca gcgcgtacag aatgacccgc cgccccgatt gcgcccctgg
     2041 tctccagagc ttgcctgggg cctagaagcg atcgcagcac gtgcactcgc caaagctcct
     2101 gccgatcgct acgactccgc ggcggcgttg gtcgaggatt tggagcggct ccgcgcggag
     2161 cgctgcgtgc gcgggcagcc ggtcccgttt tgggcccgaa cctggcactg ggcccagcgg
     2221 cacccgggtg cgcgcaatgt gattttt
//
LOCUS       DASTKU010000225         4296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_45311,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000225 DASTKU010000000
VERSION     DASTKU010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45311"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /locus_tag="VFQ61_08400"
     CDS             <1..1440
                     /locus_tag="VFQ61_08400"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HET9954511.1"
                     /translation="VFLFYFLPLVLVSYYALPFKARTLLIAVASYVFYGWANPIWALI
                     MFVGSSVDYVAGRLLLKFSGLRDDGELPPIITPDVPRTPRMKVLLALSIATNLGVLAV
                     FKYTGFVAENVNALAQLFGAREALVPVIHLVLPVGISFYTFKAMSYVIDVYRGEARPM
                     RRFTDYMCFEAFFPDLVAGPIIRYAALEEQMRVRQHTLDKFARGVAFFAFGMAKKILI
                     ANPMGNIADTAFDAGTLHAIDAWFGLVAYAFQIYFDFSGYSDMAIGLALMLGFVLIQN
                     FDSPYKSESITEFWRRWHISLSTWLRDYLYVPLGGNRRGPWRTYVNLMTVMLVGGLWH
                     GASWNFVLWGGIHGLMLAFERAQGKASAYHRLPGPLRVGVTFSIVCLAWVFFRAETLT
                     GALAYFRSLFGVSSAGEAAHAVAGALYTPYHVAVFSVAALVVWRAPNTWEFTTRLTPL
                     RAVYGVAMLGMAVLVMWTQTVNPFLYFQF"
     gene            1445..2737
                     /locus_tag="VFQ61_08405"
     CDS             1445..2737
                     /locus_tag="VFQ61_08405"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954512.1"
                     /translation="MTASSIPHRGGPTDPSHDERLRRGILFTDVRRGVAASLVAAFLG
                     LLFAVPLGQAALERVRDEESALSGLFRELPTKESLARLEDELEQTSYAKDYVQPRLQL
                     LLASVAREGNEKAVIGRAGFMFYKPGITYLASPGFLDPEVIALRERAAHEAGDKLAAD
                     PRPAILEFHAALHQRGIELVLFPVPDKAMLQPRELHGRIPSERPAPTARSRDWARLLD
                     DLKRRGVTVFDPTPATIAPGEPSRFLVQDTHWTPAFMSRVAAELAGFVKRRIQLSPPP
                     DGIAYSRRPTRVARVGDIVDMLKLTEGQTRFLPQEVEIMPVFDAAGEAFQPSESAEVL
                     LLGDSFSNIFNEDFMGWGSSAGLPAQLALELGRPLDVIAQNDSGAFATRAALALELER
                     GNARLDGKKVVIWEFASRELAVGDWKHIDWSKAMKEAP"
     gene            2734..3702
                     /locus_tag="VFQ61_08410"
     CDS             2734..3702
                     /locus_tag="VFQ61_08410"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9954513.1"
                     /translation="MNDGLHSGRRGTVLVTGAAGVMGKRLVQGLRSKGARVRGLVLPN
                     DPSRTALQALGCEIVEGDLSRPESLSALTQDITTVYHLAGVILSHDPTVFQRVNLEGT
                     AHLVRAAASSGVQHFIYVSSASVTYPRRTPYAESKWAAERVVRAQTGLHHTIVRPTLV
                     YDEHGGQEFLIFRDFLLRWPVVPFVGSGLARKRPVFSQDVIEGLLLLLENPKAYGKLY
                     NFSGGEPITLIDLARLVLRHSGQQRAFVHVPETLCRGVAKVLKSVMRRPPITESAIAG
                     LVHHADLDPAQAVADLGYCPRGVRAGFEHCFPMPRAGLPLAMKKEF"
     gene            3705..>4296
                     /locus_tag="VFQ61_08415"
     CDS             3705..>4296
                     /locus_tag="VFQ61_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954514.1"
                     /translation="MRPLVTLCLGLAAGTAALELTACSAITGAPPKGSEDAIESDQGA
                     AMAAIGKKAPGTIVWSSSRNGNHDLFTMRTDGSATKSITSGDQVDWFPRFSPDGTRIL
                     FTRSKQGWVSERDANDSEKWDTYVTNLDGSELAKLVENASWASWVDANKIVFVRGTKI
                     FEKKLPDGEEVQLMSSEGVKDLDGALLQQPGLSGDGK"
BASE COUNT          750 a         1329 c         1369 g          848 t
ORIGIN      
        1 gttttcctat tctattttct gccgctagta ctggtcagct actacgcctt gccgttcaag
       61 gcgcggactc tgctcatcgc ggttgcaagt tatgtgttct acggctgggc aaacccgatt
      121 tgggctctca tcatgttcgt cggctccagc gtcgactacg tggctggccg gctgctcctc
      181 aagttctcgg gccttcgtga cgacggcgaa ctaccgccca tcatcacgcc cgacgtgccg
      241 cgcaccccgc gcatgaaggt gttgctggcc ttgtccatcg cgaccaacct gggagtgctc
      301 gcggtgttca agtacaccgg cttcgtcgcc gagaacgtga atgcgctggc ccaactgttc
      361 ggagcgcggg aggcgctggt gccggtgatt cacctggttt tgccggttgg aatctcgttc
      421 tacacgttca aggcgatgag ctacgtaatc gacgtctatc gcggcgaggc ccgcccgatg
      481 cggcgattca cggactatat gtgcttcgaa gcattctttc ccgatctggt agccggcccc
      541 atcatccgct acgcggctct ggaagagcag atgcgcgtgc gtcagcacac gctggataag
      601 ttcgcccggg gcgtggcttt cttcgcgttc ggaatggcca agaagatctt gatcgcgaac
      661 cccatgggga acatcgcgga tacggccttc gacgcgggca ccctgcacgc gatcgatgcc
      721 tggttcgggc tcgttgccta tgccttccaa atctacttcg atttctcggg gtactcggac
      781 atggcgattg ggctcgcgct gatgctgggc ttcgtactga ttcagaactt cgattccccg
      841 tacaagtcgg agagcatcac cgagttctgg cggcgctggc acatcagcct ctccacttgg
      901 ttgcgggact atctctacgt gccgttgggc ggaaaccgca gagggccctg gcgaacctat
      961 gtaaacctca tgaccgtcat gctggtgggt ggcttgtggc acggcgccag ttggaacttc
     1021 gtgctctggg gcggaattca cggcctgatg ttggctttcg agcgggccca aggaaaggcg
     1081 agtgcctacc atcgcctgcc gggcccgctg cgcgtcggag tcaccttctc gatcgtttgc
     1141 ctcgcctggg tgttcttccg cgcggaaacg ttgacgggag cgctcgcgta ctttcgttct
     1201 ctcttcggag tgtcgagcgc gggtgaagct gcgcacgcgg tggccggcgc gctctacacg
     1261 ccttatcacg tagcggtgtt ctcggtggcc gccctggtcg tgtggcgcgc gcccaatacc
     1321 tgggaattca cgacgcgcct cacaccgctc cgcgcggtct acggcgtcgc aatgctcggt
     1381 atggcggtgc tggtcatgtg gacccaaacg gtgaacccgt tcttgtattt ccaattctga
     1441 accgatgact gcatccagca tcccccaccg gggcggaccc acggatccct cgcatgacga
     1501 gcggctgcgt cgcgggattc tcttcaccga tgttcgacgc ggcgtggccg cctcgctggt
     1561 ggcggcgttt ctcggactgc tgttcgccgt accgctcgga caagccgccc tcgagcgagt
     1621 gagagacgaa gagtccgcgc tcagtgggtt gttccgtgag ctgccgacca aagagagcct
     1681 cgcgcgactg gaggatgagc tggaacagac cagctatgcc aaggattacg tgcagccgcg
     1741 tttgcagctt ctgctcgcga gcgttgcgcg ggagggcaat gaaaaggccg tgatcgggcg
     1801 agcaggtttc atgttctaca aacccgggat cacctacctg gcgagccctg gtttcttgga
     1861 tcccgaggtc atcgcgctgc gcgagcgcgc cgctcacgaa gcgggcgaca agctggccgc
     1921 cgacccacgc ccggcgatcc tcgagtttca cgccgccctc caccaacgcg gcatagaact
     1981 cgtgttgttc cccgtgcccg acaaggccat gctgcagccg cgggagttgc acggccgaat
     2041 cccttcggag cgacccgcgc ccactgcgcg gagccgcgac tgggcccgcc ttctcgatga
     2101 cctcaagcgg cgtggggtca ccgtcttcga tccgacgcca gcaacgattg ctcccggcga
     2161 gccatcgcgg tttctggtgc aggacacgca ctggacgccc gccttcatgt cgagagtggc
     2221 ggcggagctc gcaggtttcg tgaagcggcg aatccaactc tcaccccctc ccgacggaat
     2281 tgcctattcc cgcagaccga cgcgggtcgc gcgtgtcggc gacatcgtgg acatgttgaa
     2341 gctgaccgag gggcagactc ggtttctgcc gcaagaagtc gaaatcatgc ccgtcttcga
     2401 cgccgccggc gaagcctttc agcctagtga gagcgccgag gtgctgttac tgggtgacag
     2461 cttcagcaac atcttcaatg aggacttcat gggttggggc agctccgccg ggctgcctgc
     2521 acaactcgcc ctggaactcg ggcgacctct ggatgtaatc gcgcagaacg actccggcgc
     2581 gtttgcgacg cgcgcggcgc tggcgctcga gctcgaacgc ggcaacgcgc gactcgacgg
     2641 taagaaggtg gtgatttggg agttcgcgtc acgcgagctc gcggttggcg attggaagca
     2701 catcgactgg tccaaagcca tgaaagaggc gccatgaacg acggactcca ctcgggccgc
     2761 cggggaacgg tgctagtcac gggcgccgct ggcgtgatgg gcaagcgctt ggtgcaaggg
     2821 ttgagatcca agggggctcg cgtgcgaggc ttggtgttgc ccaatgatcc gagccgaact
     2881 gcgcttcagg cgctgggttg cgagatcgtc gagggggatc tatcgcgtcc cgagtcgttg
     2941 agcgctctga cgcaggatat cacgacggtg tatcacctcg ccggagtcat tctctctcac
     3001 gaccctacgg tttttcagcg cgtgaacctg gagggcactg cgcacctggt ccgcgcggcc
     3061 gcgagcagcg gagtccagca cttcatttac gtgtcctccg cgtccgtcac ttacccgcgt
     3121 cgtacgccgt acgccgagtc caaatgggcg gccgaaaggg tggtgcgggc ccaaaccggg
     3181 cttcatcaca cgatcgttcg acctaccctg gtgtacgacg agcacggcgg gcaagagttc
     3241 ctgatcttcc gagacttcct gctgcgctgg cccgtggtgc cgttcgtcgg ctcgggtctc
     3301 gcgcgcaagc gaccagtgtt cagccaagac gtgatcgagg gtctgctgct gctcctcgaa
     3361 aatccgaagg cctacggcaa actctataat ttcagcgggg gcgagccgat tacgctgatt
     3421 gatttggctc ggctcgtact caggcactcg ggccagcaac gtgcgttcgt gcacgttccg
     3481 gagacgctgt gccgcggagt cgccaaggtt ttgaaaagcg tgatgcggcg gcccccgatt
     3541 acggagagtg cgatcgccgg gctcgtccac cacgctgatc tcgatcctgc acaggcggtc
     3601 gcggatctcg gctactgccc tcgcggagta cgcgccggct tcgaacactg ctttcccatg
     3661 ccccgcgcgg ggctgcctct cgcaatgaag aaggagttct gaacatgaga ccgctcgtca
     3721 cgttgtgctt aggactagca gcgggtacgg ccgcgctgga gctgaccgcc tgctcggcaa
     3781 tcaccggcgc cccgccgaag ggctccgaag acgcaatcga gagcgaccag ggcgccgcca
     3841 tggccgcgat tggaaagaag gcgcccggca ccatcgtttg gtcgagctcg cgaaacggca
     3901 atcacgattt gttcacgatg cgaacggatg gctccgcaac caagagcatc acctcggggg
     3961 atcaggtcga ctggtttccc cgcttctcgc cagatggcac cagaatcctg tttacccgca
     4021 gcaagcaggg ctgggtgagc gagcgtgacg ccaacgatag cgagaagtgg gacacctacg
     4081 tcaccaatct cgatggctcc gagctcgcca agctggtcga gaacgcgagc tgggcgagct
     4141 gggtcgatgc caacaagatc gtgttcgtgc gcggcaccaa gatcttcgag aagaagctgc
     4201 ccgacggcga agaggtgcag ctgatgagca gcgaaggggt caaagatcta gatggcgccc
     4261 tgctgcaaca acccgggctc tcgggggacg gaaagt
//
LOCUS       DASTKU010000226        12598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1829633, whole genome shotgun sequence.
ACCESSION   DASTKU010000226 DASTKU010000000
VERSION     DASTKU010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12598
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829633"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFQ61_08420"
     CDS             complement(<1..734)
                     /locus_tag="VFQ61_08420"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HET9954515.1"
                     /translation="MNKRVRMQRNLVAVVCVAWLSAACGAAVPPKELVDARAAYERAS
                     KSRANELAPAELDTAKQSLGRAEGSFLDNGDDPLTRDLAYIAERKAMAAEAQAGILEA
                     ERQRDATDKDFKKAQLATIDKTKAQLEEEKRAREATRAEADKTKAQLETEKKAREEAE
                     KRASAAMASLAEIAKVKEESRGVVITLSGSVLFATGQSDLLPIAKDKLNEVAKALKDQ
                     GFKQLTVEGHTDSRGNAAKNEELSLRR"
     gene            complement(731..1090)
                     /locus_tag="VFQ61_08425"
     CDS             complement(731..1090)
                     /locus_tag="VFQ61_08425"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HET9954516.1"
                     /translation="MTRRVFSAPWILSFLLLACGGAPLPQEQFSAAQASVKGAEVAGA
                     AEDPKSALHLKLAHEQLDKAKALMDNGDNERAAHVIDRAQADAELAILLAKEARARKE
                     ATDAREQVEELKQRIQK"
     gene            complement(1419..1664)
                     /locus_tag="VFQ61_08430"
     CDS             complement(1419..1664)
                     /locus_tag="VFQ61_08430"
                     /inference="COORDINATES: protein motif:HMM:NF018545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HET9954517.1"
                     /translation="MTDNERTELEAAAFRRLVAHLRERTDVQNIDLMNLAGFCRNCLS
                     KWYREAALERGQELSDGDAREIVYGMPYAEWKTRYQK"
     gene            complement(1701..3950)
                     /locus_tag="VFQ61_08435"
     CDS             complement(1701..3950)
                     /locus_tag="VFQ61_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET9954518.1"
                     /translation="MLVRALAVGHGPARMLPYQNAEIGIDERLDDLLSRMTLPEKVGQ
                     MLQLDGRQDCVALVEKYAPGSLLHVLDERLDRAMQAAARTRLGIPLLVGEDGIHGHSF
                     HAGATIFPTQLGLACSWNPDLLRDVARVTAREMALTGAHWTFSPVLCIARDLRWGRVG
                     ETFGEDAFLIGELGAAMIEGYQGRGLNDPEAVLATAKHYAGYSESQGGFDASEGELSR
                     RKLRTYFFPPFERAARAGCRAFMTGYNAMDGLPSTANRWLLREVLREEWAFEGVVVTD
                     WDNVGRLHWGQRIVPSILEAAVVAVRAGNDVIMVTPDFYEAAQQAVAQGLLAESEIDV
                     VVRRILRLKFELGLFENPRAPRFAEQAAVIGCAPHREVALRAARESLVLLQNDGLLPL
                     SAERLRHISVIGPNADDDLAQLGDWSLGASQYPPDFGKHPRECSTTILDGVRARVPQV
                     QVSSCKGCSSVEAGLEGLAEARALAEASDVCVLVLGDSLPFNGETNSTATLELMGGQK
                     ALLAAVASTGKPFVLVLINGKPLVLPEEAQRASAIVECFNPGMLGGTAVAELLFGDLN
                     PIGKLPISIPRHVGQTPIAYTRLEHRHGKNYADLSAQPAFPFGHGLSYTQFLYSNLTT
                     STPVLEAGQTAVVEFDLANVGEREGIEIVQLYVSDLVASVTWPRQLLQAFTRVGLQPG
                     EKRRVRLEVPFERLALIDAFERRVVEPGHFELRVGGSSRRTDQLAVEIEVLGQVDPLS
                     RIPGILPEN"
     gene            complement(4047..4880)
                     /locus_tag="VFQ61_08440"
     CDS             complement(4047..4880)
                     /locus_tag="VFQ61_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9954519.1"
                     /translation="MRSRVVVALVFAASLSARVGRAEPHELKRTEPLTLGIELGLSAV
                     AMSAGILAPNPKQCRWCSPPGLDEAVAGWFEIEGRRPAAQLSHVLSFSVLPAASVAAL
                     VAPALSQSAPTRVALEDVSIALQAMGWNLALSQAVKKLVARRRPAFFYERSRLTEYPD
                     SLKQRNQSFFSGDTSSAFVMASSVTTLAFLRGYSWAPYAAAAGGTLATATGLLRIAAD
                     VHWASDVFVGAVAGSAIGCLVPILLHPRAARNKEVSSDGALLLPEFGQSMTGLSWAGQ
                     F"
     gene            5052..6083
                     /locus_tag="VFQ61_08445"
     CDS             5052..6083
                     /locus_tag="VFQ61_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954520.1"
                     /translation="MSLPSVRLLSAGLTIGLLLRPVSAPAQPQSSAESPRERARALAT
                     QGYEALQAKDYRAAEDLFRRADALVHAPTIVVDHARALVGLGRLVEAHEKYELVLREG
                     IPGDAPWSWRRAQQEAERELEAIQPRLAWLTVVVRGPVKPLVYLDGRQVPIAALGVRR
                     ATDPGARSLRVSGAGYLPYQATLQLKEGETRAVQIELKRDASGELPLEEEKARGPVVV
                     STAPATPASSVAAYVALGLGGAGVLAGTISGMRALEVRSALKSECGGRVCVPSTEQEL
                     ADKQSRIDKYRLLGTISGVSFAIGAAALATSGVLFVLRPRETSRPDPSATLLLPYVGA
                     REAGVVGQF"
     gene            6300..7298
                     /locus_tag="VFQ61_08450"
     CDS             6300..7298
                     /locus_tag="VFQ61_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954521.1"
                     /translation="MTAKILLGIFALATWLVPVGSVCAQVNEADRATARVLAAEGNEA
                     LTRKDFVTAEDRFRRADTLVHAPSLVLDHARALVGLGRFAEAYRRYSLVLQERLPEGA
                     PAAWKRAVKEAEQEIQSVRPRVGWLTLRISGAPDPHVWLDGRELSEDDLRSTIPVDPG
                     RRSVSVRAPGFKSSEGEITVREGAESELAVTLNPLPTEAPPPPEPRVVVAPPAPAPPA
                     KNKVLAYTSLGIGAAGLVAGTVTGVLFLGVLSDIKSECPDLHCEYGGADRVAHHEQQE
                     RRYKLLGTLSGASFIVGIAGATVGTVLLVSSGSHKTEKASVSAYVGAGALGLAGRF"
     gene            7298..9982
                     /locus_tag="VFQ61_08455"
     CDS             7298..9982
                     /locus_tag="VFQ61_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954522.1"
                     /translation="MLKAKLAYRALFVLCIAAWLSACQVLFGEVTIERSPPGTSSGAC
                     TVGSFRCNSEFLLSCDGSSGPDWFLKETCASAERCDSKLQKCQTCKPGDLRCMGAVRE
                     ECSSDGTAWLAKLPACDSASQCNPTGCFPCIPKTHQCRGPEGRELWECDDSGEWTLLV
                     ETCVTDALCKSSAERADTSPNWERRCDPPGCTEAGKYMCTGTLLQRCRQDLSGWAPVD
                     TCLSKELCDKAATLANESPAGGAALDMCPRGCEDPQAHLCDGMTLKSCAPDLTGWVPQ
                     MTCPPGTECNPDQGTCSALCDPGATQCNGAQLRRCSPQRTWEVLEDCKSDALCQASLA
                     ATSPLTTPHCIPPTCPTAGDYYCDGARPFKCNNDLTQWEPQPPCDSAALCEAQNKRCR
                     ERACDPGQLRCFDNELRRCRDDLTQFDPYMTCGSGKFCSDSSGSEGCWDKCPTGTVCS
                     NGTDLWECGGDPLLPTNRIAACITSSLCVPGQRECKPPICSVGEEKCENNKLLRCSAG
                     RDKWDVTDCNQTQTTCVSGTAMSPGVCARCPANASECNGNTLRYCSSDRTKWETRECV
                     DKTPVCDAANRECDACVANSTTCDGQTLVQCDAQGHSATRTTCTGTTPVCDAAGRQCD
                     VCTAKSARCSGNVLYTCNDTGQKETATECATAQLCDAVDGCKAPVCAKGDTRCNGAQP
                     EVCNAGRTAYVAMGEPCATKELCVGSTGTCTPPACAANGKQCDGAQPQVCNAGRTAWV
                     NSGTACATAALCVSGACKNPTCAATDFQCDGSWLQTCNAGRTDFENNKQCGRAALCDA
                     KAGRCKACLAGEFSCDGAKLQTCNDTLSGWNDVTTCTAANLCDATNGECDECLSGDKK
                     CDGNKLLSCDTAGHFVLEVDCDTTAQNCSDDEKACVLP"
     gene            9954..11366
                     /locus_tag="VFQ61_08460"
     CDS             9954..11366
                     /locus_tag="VFQ61_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954523.1"
                     /translation="MMKKPACSRNGWRHFRHGALSLLLLPLGCQLVFGDFRVDTRELY
                     PALENTTCEFGELRCEEGALTVCASDRTSTNQIVQCSTPEQCNLNAQGCQPCKEGERQ
                     CNGAFLEVCGTDGKFQRTACESAALCRASGCLSPVCKAGEHRCNGGRLERCAPGRDGW
                     QLLSVCASATQCDAARADAQSIAGMPATCASPVCRANQFRCDGPQPQACKATLDAWEN
                     KGAACAPGTTCNPADGTCTPCKPGTQTCSGKDLLNCTAEGRFELQESCGSVELCRGGA
                     SKSSCLTSGKCDVPGQVTCDAGIIQRCAEGLVWETLVTCLSPAYCAEGRIRCGAPACG
                     PNGFRCMGNTLLKCNGDGSSLELVQACDAGLQCDSTVGTCALPCTNGSQRCNDRFFET
                     CEQQTWKVTADCGSAALCRTSGTPGCLAPVCGGDLPEFSCANDQPQQCKPGRDGFVAK
                     GRPCTSDERCDSVLGRCTPL"
     gene            11363..12403
                     /locus_tag="VFQ61_08465"
     CDS             11363..12403
                     /locus_tag="VFQ61_08465"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET9954524.1"
                     /translation="MTTPGRTVHHRYSDPLDLIWLRAAARLGMHVERSSEVYASWDGV
                     RKLQLAESADFDADDSLAQLIFHEICHALVAGPSGRRQPDWGLDNFSDRDLVLEHACH
                     RVQASLAASYGLREFFAVTTQWRGYWDALPQDPLEPSDDPAIPIARRAMQEATSPPFS
                     EVLRDALEATQRIADLVREVAPPDSLWRQTRASHPSGFQFHPDAGLRCRECAWSLGKG
                     NFRRCRQALRLHRSTRMSRELAACERWEPRLTDESCMTCGACCREGFDRVELGAKDDG
                     LRKRHLQLISEDRFGEFLARPDGRCVALTGDGSSAAFRCTIYAERPRSCADFGVASAA
                     CLIARRRVRLSA"
BASE COUNT         2292 a         4069 c         3982 g         2255 t
ORIGIN      
        1 cgtcgcaggc tgagctcctc gttcttcgcc gcgttgcctc gtgagtccgt gtggccctcg
       61 accgtcagct gcttgaagcc ttggtccttg agcgccttcg cgacctcgtt caacttgtct
      121 ttggcaatcg gcagcaaatc actctggccg gtggcgaaca acacgcttcc ggagagagtg
      181 attacgacgc cgcgtgactc ctctttgacc ttcgcgatct cggctaaact agccatcgcc
      241 gccgaggcgc gcttctccgc ctcttcacga gccttctttt cggtctcgag ctgcgccttc
      301 gtcttgtcgg cctcggcgcg ggtcgcttcg cgcgctctct tctcttcctc gagctgcgcc
      361 ttcgtcttgt cgatcgtcgc cagctgggct ttcttgaaat ccttgtcggt ggcgtcacgc
      421 tgacgctctg cttcgaggat gccggcctga gcctctgcgg ccatagcctt gcgctcggcg
      481 atgtacgcca agtcacgcgt cagcggatcg tccccattgt cgaggaagct gccttcggct
      541 cgaccgagtg actgcttggc cgtgtcgagt tcggcaggcg ccaactcgtt cgcccggctc
      601 ttcgaggcgc gctcgtaagc cgctcgggca tcgaccagct ccttgggtgg caccgctgcg
      661 ccacacgccg ccgagagcca cgcgacgcaa acgactgcaa ccaggtttct ctgcatgcgc
      721 acgcgcttgt tcatttttga atcctctgct tgagctcttc gacctgttcc cgggcgtccg
      781 tcgcttcctt acgagcgcgc gcttctttgg cgagcaaaat ggcgagctcc gcgtcggcct
      841 gcgcacgatc gatgacgtgc gcggcccgtt cgttgtcgcc gttgtccatg agcgccttcg
      901 ccttatcgag ctgctcgtga gccaatttga ggtgcagcgc ggacttggga tcctccgccg
      961 cgcccgcgac ctcggcgcct ttgaccgaag cctgtgcagc agagaactgc tcctgcggca
     1021 gtggcgcacc accacatgcc aaaagcaaga atgagagtat ccaaggtgcc gaaaagactc
     1081 gtctcgtcat ttcagaacgc atcaaagccc aagcgccggt tttcgtaaag gtccagctcg
     1141 cgtcgtccga caggacacgc cttcgcggcg gggcagtgcg gccgagccct cgctggtgag
     1201 cggaaacgcg agccccgcat tcgggtccgg tgtgggcgaa tcgaaggcca cgcggccccg
     1261 cagggagact gggctcgcca cggaagattt tagcgctgct gatgttcgcc ggtgtgcgac
     1321 tgcgcttcat cccgcagcag cgcagccgtc ttcgcgcttc gttgtcttct gtgcttcgcg
     1381 ttctttgccc ttcgccgcgt ttttccgctt cgcggcgctc acttttgata gcgagtcttc
     1441 cactccgcgt agggcatgcc gtacacaatc tcgcgcgcat ccccgtcgct gagctcttgg
     1501 ccccgctcca gggccgcctc gcgataccac ttagacaggc aatttctgca gaatccggcc
     1561 agattcatca aatcgatgtt ctgcacgtcg gtgcgctcgc gtaagtgggc aaccaggcgt
     1621 cgaaaagctg cggcctcgag ttcggtacgt tcgttgtcag tcatggcgac gacctgaacc
     1681 taccccaacc acacgattcc tcaattctcg ggcagaatcc ccggaatccg cgatagcggg
     1741 tcgacttgac cgagcacctc tatttcgacg gcgagctggt ccgtccgcct cgaagagccc
     1801 ccgacgcgga gctcgaaatg tcccggttcc accacccgtc gttcgaacgc atcaatcaac
     1861 gccaagcgtt cgaacggcac ctcgagtcgc acccgacgct tctctcccgg ctgcagcccc
     1921 acccgcgtaa acgcctgtaa gagctgccta ggccaggtga cgctcgccac caaatcgctg
     1981 acatacaact gaacgatttc gatgccctct cgctccccca cgttcgctag atcgaactcc
     2041 acgaccgcag tttgccccgc ttcgagcacg ggggtcgagg tggtgaggtt cgaatagagg
     2101 aactgtgtgt aagacagccc atgaccaaag gggaatgcgg gctgcgcgct cagatccgcg
     2161 tagtttttgc cgtgcctgtg ttcgagccgc gtgtaagcaa ttggcgtttg ccccacgtgc
     2221 cgcggaatgc tgatggggag cttgccgatg gggttcaagt ccccgaagag aagctctgcc
     2281 accgcggtgc cgcccaacat gccggggttg aaacactcca cgatcgcgct ggcacgctgc
     2341 gcttcctccg gaagcaccag aggcttgcca ttaatcaaaa ccagcacgaa cggcttaccc
     2401 gtactggcca cggcggcgag cagggccttc tgcccgccca tcaactccag ggtggccgtg
     2461 ctgttggttt caccgttgaa cggcaggctg tcgcccaaca ccaacacgca cacatcgctc
     2521 gcctccgcga gcgcgcgtgc ctccgcgagt ccttccaaac ccgcctcgac actgctgcag
     2581 cctttgcagc tcgagacctg aacttgcggc acacgagcgc gcactccgtc gagaatcgtc
     2641 gtgctgcatt cgcgcgggtg cttgccgaag tccggtggat attgcgacgc tccgagggac
     2701 caatcaccga gctgggctag gtcgtcgtcg gcgttggggc cgatcacgga aatgtgacgg
     2761 aggcgctccg ccgagagagg caacaggccg tcattctgca gcaacaccaa gctctcacgg
     2821 gcggcccgca aagccacttc gcggtgcgga gcgcaaccaa tgacggccgc ctgctccgcg
     2881 aagcgcggtg cgcgcgggtt ctcgaacagg ccaagctcga atttcaggcg caagatgcgg
     2941 cgtacgacca cgtcgatctc cgactctgcc agcagtccct gtgccacggc ctgctgggcc
     3001 gcctcgtaga agtcgggcgt caccatgatt acgtcgttac cggcgcgcac cgctaccacc
     3061 gcggcttcca aaatcgaggg cacgattcgc tgcccccaat gcaaccgacc gacgttgtcc
     3121 cagtcggtta cgaccacgcc ctcgaacgcc cactcctctc gcaacacttc gcgcagcaac
     3181 cagcggttcg ccgtcgaagg cagcccgtcc atggcgttgt atccggtcat gaaggcgcga
     3241 cacccggcgc gcgctgcgcg ctcgaacggc ggaaagaagt aagtcctcag tttgcgccgc
     3301 gagagctcgc cttcggacgc gtcgaaccct ccctggctct ccgagtagcc ggcgtagtgc
     3361 ttggccgtcg cgagcaccgc ttctggatcg ttcaagccac gcccctgata gccctcgatc
     3421 atagcggcgc cgagctcacc gatcaggaac gcatcctcgc cgaaggtctc gccgacacgc
     3481 ccccaacgta gatcgcgtgc gatgcacaat accggagaga aagtccaatg cgctcccgtc
     3541 agcgccatct cgcgtgcggt gacccgagcc acgtcgcgca gcaagtccgg gttccaggaa
     3601 cacgcgaggc ccagctgagt gggaaagatc gtggcgccgg cgtgaaacga atgcccgtga
     3661 atgccgtcct ccccgaccag cagcgggatc ccgaggcggg tccgcgccgc ggcctgcatg
     3721 gcgcgatcca aacgttcatc caggacgtgc aaaagcgagc ccggcgcata cttctcgacc
     3781 aaagcgacgc agtcctgtcg tccatcgagc tgcagcatct gccccacctt ctctggcaga
     3841 gtcatccgcg acagcaggtc gtctaggcgt tcatcgatgc cgatctccgc gttctggtaa
     3901 ggcagcatgc gggccggacc atggccgacc gccaacgctc tgacaagcac tctgacggct
     3961 gtgtctcgtt cgagcggtgc ggagtcccca ctctccgcat tttcgctcac atttcgagca
     4021 ccggagcgtg ctcgggtctc gggatgtcag aattgaccag cccaagacag ccccgtcatc
     4081 gactgcccga actccggcag gagcagcgcg ccatcgctcg agacttcctt gttccgcgcc
     4141 gcacggggat gcagcaggat cgggaccaaa cagccgattg cgctgcccgc caccgcgccg
     4201 acgaacacat cgctcgccca atgcacgtcc gccgcgatcc ggagtagccc ggtcgccgtt
     4261 gccaacgtgc cccctgcggc cgcggcgtaa ggggcccagg aatagcctct caaaaaggcg
     4321 agggtggtga ccgagctagc catcacgaag gcgctgctgg tgtcacccga gaagaacgac
     4381 tgattgcgct gcttgagact atccgggtac tcggtgagcc gagaacgctc atagaaaaag
     4441 gccggtcgtc gccgagccac cagcttcttg acggcctgac tcaaggccag gttccacccc
     4501 atcgcctgca acgcgatact gacgtcctcg agggcgacgc gagtgggagc agactggctc
     4561 agggccggag ccacgagcgc ggcaaccgaa gccgccggca agacggaaaa ggacaacacg
     4621 tggctcagct gcgcggctgg acggcggccc tcgatctcga accatcccgc cacggcttcg
     4681 tccaacccgg gaggcgagca ccagcgacac tgcttcggat tcggcgcgag gatccccgcg
     4741 ctcatggcca ccgcggagag ccccaattcg atgccaagag tcagaggctc cgtgcgcttc
     4801 aattcgtgag gctcggctcg acccacgcga gcagacaggc tggccgcaaa caccaaagca
     4861 acaacgaccc tcgaccgcat cgccgtccta catacaacac cgtgagcacc caagacaaag
     4921 gtccgtgggg tctctggccc gaaccgtaga cgaatcggtc tgctcgaatg cagccctgat
     4981 ttgcacgccg tctacttctc gactgctagt tattgctgcc tcgcgcagcg ccgtgtacac
     5041 ggggacgagc catgtcgctg ccctctgtcc gcctgttgag cgccgggctc acgattggcc
     5101 tgctgcttcg gcccgtctcc gcgccggcac agccccaatc cagcgccgag agcccccgcg
     5161 aacgcgcgcg agcactggcg actcagggct acgaggccct acaagccaag gactaccgag
     5221 cggcggagga cctgttccga cgtgccgatg cgctagtgca tgcgcctacc atcgtggtgg
     5281 accacgcgcg agcgctagtg ggtcttgggc gcctggtcga agctcacgag aagtacgaat
     5341 tggtgttgcg ggaggggatc cccggggacg ccccctggtc atggcgtcgg gcccaacaag
     5401 aggccgaacg cgagctcgaa gccatccaac cgcggctggc ttggctgacg gtggtagttc
     5461 gcggccccgt gaagccactc gtatacttgg atggtcgcca ggtgccgatt gcagctttgg
     5521 gcgtgcgacg cgccaccgat ccgggagcac gctctctacg cgtctcgggc gccgggtacc
     5581 tgccttatca agccaccttg cagctaaaag agggtgaaac tcgcgccgtt cagattgagc
     5641 tgaagcgaga cgcgagcggc gaacttcccc tagaggaaga gaaagcgcgg ggtccagtcg
     5701 tagtctcgac tgcgccggcc acgccggctt cctcggtggc agcgtacgtt gcgcttggcc
     5761 tcggcggggc aggagtactc gcaggcacca tcagtggcat gcgggctttg gaggtgcgca
     5821 gcgcgctgaa gagcgagtgc ggcggccgag tctgtgtccc gagcaccgaa caggagctcg
     5881 cggataaaca gagtcgaatc gacaagtacc gactcttggg gaccatctcg ggagtgagtt
     5941 tcgccattgg cgcggcggca ctcgccacat cgggggtgtt gttcgtgctg cgcccgagag
     6001 agacaagccg cccagatccg agcgccacgt tgctcctacc ctacgtgggc gcgcgtgaag
     6061 ctggcgtggt tggacagttc tgaacgcacc ccaacgccgt cggagcgagg ctccgttgat
     6121 tccgagcaag gccgagcgcc tgttccttcg ggaggccggc cagtgctgct tgcgaggcac
     6181 ccggaaaaga gggtaagcgt acttaactcg cgttgcgcta tctggctgcc tcagggaaaa
     6241 atgggtttgc gcccggctcg tcgggagcga gggtccacct gatttccaag ggaacgaaag
     6301 tgacggcgaa aatcctactt ggcatcttcg ctctcgcaac ttggctcgtc cctgtcggca
     6361 gcgtctgcgc gcaggtcaac gaggccgacc gggccaccgc gcgagtgctg gccgcggagg
     6421 ggaacgaggc tcttacccga aaagacttcg tcaccgcgga ggaccgtttc cggcgagcag
     6481 acacgttggt tcacgccccg agcctggtgc tcgatcacgc gcgcgcgctg gtgggcctgg
     6541 gccgttttgc ggaagcctat cggcgttatt cgctggtgct ccaagagcga ttacccgaag
     6601 gcgccccggc cgcttggaaa cgggctgtaa aagaggcgga gcaggaaatc caatcggtgc
     6661 gtccgcgtgt cgggtggctg acgctacgaa tcagcggcgc gccggaccct cacgtctggt
     6721 tagatggtcg ggaattgagc gaggacgacc tgcggtcgac catccccgtc gatcctggac
     6781 gacgttcggt cagcgtgcgg gcacctgggt tcaagtcgag cgagggggag attaccgttc
     6841 gcgagggcgc cgagtcggag ctcgcggtta cgctgaaccc cctccccacc gaagctccac
     6901 ccccacccga gccgcgtgtg gtggttgccc cacccgcgcc agcgccaccc gcaaagaata
     6961 aggtattggc atatacgtcc ctaggcattg gtgcggccgg gctcgtagct ggcacggtaa
     7021 cgggcgtctt gtttttgggg gtgctctcgg acatcaagtc ggagtgtccc gacttgcatt
     7081 gcgaatacgg cggcgccgac cgcgtcgctc atcatgagca acaagagcga cgttataagc
     7141 tgcttggaac tttgtcgggg gcaagtttta tcgtggggat cgcgggggcg accgtgggga
     7201 cggtgttgct cgtctcctcg ggttcgcaca aaaccgagaa ggcgagcgtc tcggcgtatg
     7261 tcggcgcggg agcgcttggg cttgcgggaa ggttctgatg ctgaaggcga aactcgcgta
     7321 tcgcgctcta ttcgtgctgt gcatcgcggc gtggctcagc gcctgtcagg tcttgttcgg
     7381 tgaggtcacg atcgagcgct ccccgcccgg aaccagctcg ggcgcatgta cggtaggcag
     7441 cttccggtgc aacagtgagt tcttactgtc ttgtgacggc tcgagcggcc cagattggtt
     7501 tctaaaggaa acttgtgcga gcgccgagcg ttgtgattcc aagctccaga agtgccaaac
     7561 gtgcaagccc ggcgatcttc gctgtatggg cgcggtgcgc gaggagtgct cgtcggatgg
     7621 cacggcctgg ttagcgaagc tgcccgcgtg cgactccgcg agccaatgca atcccacggg
     7681 ttgcttcccc tgcatcccca agacgcatca atgtcgcgga ccggagggtc gcgaactctg
     7741 ggagtgcgac gactccgggg agtggacgct cctcgtcgag acttgcgtta cggatgctct
     7801 ttgtaagagc agcgccgaac gtgcggacac gagcccgaac tgggagaggc gctgcgatcc
     7861 gccgggctgc accgaggcgg gcaagtatat gtgcacgggc acgctgctgc agcgttgccg
     7921 gcaagacttg agcggttggg ctcccgtcga cacatgcctg agcaaggagc tctgcgacaa
     7981 agcggcgacg ctcgcgaacg aaagtccagc gggcggcgcg gccttggata tgtgccctcg
     8041 cggctgcgaa gacccacaag cgcacctctg cgacggcatg actctcaaga gctgcgcacc
     8101 tgacctcacc ggatgggtgc cccagatgac gtgccctccg ggcacggagt gcaatcccga
     8161 ccaggggact tgctcagcgc tctgcgatcc gggagcaaca cagtgtaacg gcgctcagct
     8221 gcgccgctgt tcgccccaac ggacctggga ggtgctcgag gattgtaagt ccgatgcgct
     8281 ctgccaagcc tcgctggctg ccacgagccc actcactact cctcattgca tacctcccac
     8341 ctgccccacc gcgggcgact actactgcga cggggctcgg cccttcaagt gcaacaacga
     8401 cctaacgcaa tgggagccgc aaccgccttg tgactcagcg gcgttatgtg aagcgcagaa
     8461 taagcgctgt cgggaacgcg cctgcgatcc ggggcaactg cgctgcttcg acaacgagct
     8521 gcgccgctgt cgagacgatc tcactcaatt cgacccgtac atgacgtgcg ggagcggcaa
     8581 gttctgcagc gactcctcgg gtagcgaggg ctgctgggat aagtgcccca caggtacggt
     8641 ctgctcgaat gggacggatc tttgggaatg cgggggagac cccttgcttc ccaccaatcg
     8701 catcgcggcc tgcatcacgt cctccctctg tgttccgggc caacgagagt gcaaacctcc
     8761 gatttgttcc gttggcgaag agaagtgcga gaacaataag ctgcttcgtt gctcggcggg
     8821 gcgggacaag tgggacgtca cggactgcaa ccagacgcag acaacgtgcg tctccggcac
     8881 ggcaatgtcc ccgggagtct gtgcgcggtg cccggcgaac gcgagcgagt gcaacggaaa
     8941 cacgctgcgc tattgctcgt cggatcgaac caagtgggaa acgagggagt gcgtcgataa
     9001 gacgccggtt tgtgacgccg ccaatcgtga atgcgacgcc tgcgtcgcca actccacgac
     9061 ttgtgatggt cagaccctcg tgcaatgcga tgcgcaaggg cacagcgcga cgcgcacgac
     9121 ctgtacgggc accacgcccg tttgcgacgc agcaggtcga caatgcgacg tttgcaccgc
     9181 gaaaagcgcg cgttgctcgg gaaacgtcct ctatacgtgc aacgacacgg ggcaaaaaga
     9241 gaccgccacc gagtgcgcca ccgcgcagct ctgcgacgcg gtggacggat gcaaggcccc
     9301 cgtctgcgcc aagggcgata cgcgctgtaa cggcgcgcag cccgaagttt gcaacgccgg
     9361 ccgcaccgcg tacgtcgcga tgggcgaacc ctgcgccacg aaggagcttt gtgtcggcag
     9421 cacgggcaca tgcacgcctc cggcctgtgc ggccaatggc aagcagtgcg acggcgctca
     9481 accccaagta tgcaacgccg ggcgcacggc atgggtcaac tcgggcacgg cctgcgccac
     9541 ggcagctctc tgcgtgagtg gagcctgcaa gaaccccacc tgtgccgcca cggacttcca
     9601 atgcgatggg tcgtggctcc agacctgcaa cgcgggtcga acggatttcg agaacaacaa
     9661 gcaatgcgga cgtgcggcgc tgtgtgacgc gaaagcgggc cgctgcaagg cttgtttggc
     9721 cggagagttc agctgcgacg gcgccaagct gcagacctgc aatgacaccc tgagtggttg
     9781 gaacgacgtg accacgtgca cggctgccaa cctgtgtgat gcgaccaacg gcgagtgtga
     9841 tgagtgcctg agcggagaca agaagtgcga cggcaacaaa ctcctctcgt gcgacaccgc
     9901 ggggcacttc gtgctcgaag tggactgcga cacgactgct cagaactgta gcgatgatga
     9961 aaaagcctgc gtgctcccgt aacgggtggc gtcacttccg gcacggcgcg ctctcgctgc
    10021 tgctgcttcc cctgggttgt cagctggtgt ttggggattt ccgggtggac acgcgcgagc
    10081 tctaccccgc gctggaaaac acgacgtgtg agttcggtga gctacgttgc gaagagggcg
    10141 cgctcacggt gtgcgcgtcc gatcgcactt cgacgaatca gatagtgcag tgctcgacac
    10201 ccgagcagtg caacttgaac gcgcaaggtt gccagccctg caaagaggga gagcggcaat
    10261 gcaacggcgc gttcctcgag gtctgcggca ctgacggtaa gttccaacgc accgcttgcg
    10321 agagcgccgc gctgtgccga gcaagtggct gcttgagtcc cgtctgcaaa gccggggaac
    10381 atcgctgcaa cgggggtcgc ctagagcgtt gtgcaccggg tcgcgatggg tggcaattgc
    10441 tcagcgtctg tgccagcgca acgcagtgcg acgccgcgcg cgcggatgct caatcgatcg
    10501 cgggaatgcc cgccacgtgt gcgagcccag tgtgccgagc caaccagttt cgctgcgatg
    10561 gaccgcagcc gcaagcttgt aaagccaccc tcgatgcttg ggagaacaag ggcgccgctt
    10621 gtgccccggg caccacctgc aatcccgccg acggaacttg caccccgtgc aaaccaggca
    10681 cccaaacctg cagcggcaaa gacctactca actgtacggc ggagggccgc ttcgaacttc
    10741 aggagagctg cggctcagtg gagctttgcc ggggcggtgc atcgaaatcg agctgcctca
    10801 ctagcggcaa gtgcgacgtc cccggtcaag tcacgtgtga tgcaggcatc attcagcgct
    10861 gcgccgaagg cttggtttgg gagaccctcg tcacctgcct gagcccggcg tactgcgcgg
    10921 agggtcgaat tcgctgcgga gcccccgcgt gcggacccaa cgggttccgc tgcatgggta
    10981 acactctgct caaatgcaac ggggacggct cctccctgga actagtgcaa gcctgcgacg
    11041 ctggcctgca atgtgattcg acggtaggta cctgcgccct gccctgcacc aacggttcac
    11101 aacgctgcaa cgatcgtttc ttcgagacct gcgagcagca aacctggaaa gtgaccgccg
    11161 attgcggcag cgcggcgcta tgccggacga gcggaacacc gggctgcttg gcccccgttt
    11221 gcggcggcga cttgcccgaa ttcagctgcg cgaatgacca accgcagcaa tgcaaaccag
    11281 gtcgggatgg cttcgtcgcc aagggtcgac cctgcaccag cgacgagcgt tgtgattcgg
    11341 tgctaggccg ttgcaccccg ttgtgaccac tcctggccga accgttcatc accgctactc
    11401 tgaccccctg gatctaatct ggctgcgcgc cgccgcgcgc ttgggaatgc acgtggagcg
    11461 gtcgtcagaa gtgtatgcct cgtgggatgg cgtacgtaag ctgcagctgg ccgaaagtgc
    11521 tgacttcgac gccgacgaca gcctggctca gctgatcttc cacgagattt gtcatgcgct
    11581 cgtggcgggg cccagcggac gtcggcagcc tgactggggg ctcgacaact tctccgatcg
    11641 tgatttggtg ctcgagcacg cttgccaccg agttcaagcg agcttggcgg ccagctatgg
    11701 cctgcgagag ttcttcgccg tgaccacgca gtggcgcggc tattgggacg cgctgcccca
    11761 agatccgctg gaaccgagcg atgatccggc gattccgatc gcgcggcgag ccatgcaaga
    11821 agccacctcg cccccctttt cggaagtgct gcgagacgca ttggaggcca cccaacgaat
    11881 cgcggatctc gtgcgggagg tggccccgcc ggacagcctg tggcggcaaa cacgcgcgag
    11941 ccacccgagt ggatttcagt ttcacccgga cgcggggtta cgttgtcggg aatgtgcttg
    12001 gtcgcttggg aaaggcaatt ttcgccgctg ccgccaagca ctgcgcttgc accgaagcac
    12061 gcgcatgagt cgggagctcg ccgcctgtga gcgttgggag ccgcgcctga ccgacgagtc
    12121 ttgcatgact tgtggcgcgt gttgccgcga aggtttcgat cgcgtcgagc ttggtgcgaa
    12181 ggacgacgga ttacgcaaac ggcacctgca gctcatctcc gaagaccgct tcggcgaatt
    12241 cctggcccgc ccggacgggc gctgcgtagc tctgaccggt gatggctcct cagcggcgtt
    12301 tcgctgcacg atatatgcag agcgaccgcg ttcgtgcgcc gacttcggcg tggcgtccgc
    12361 ggcatgcctg attgcgcggc gccgggtacg actgagcgcc tgatcgagaa ctgcgcgcat
    12421 cggcccgagg acgagtaacg agcacgaggc ctaaatgccg tagtcggggc cccgagcggg
    12481 cttgcgcttc gttgccgccg gcgctttgcg tttggcctca gcttcacgag ccgcaccgct
    12541 gtttcgtggg gcgggatcat cacccgttag gttgacgatt ggcgcagcgc tcgggatc
//
LOCUS       DASTKU010000227         5412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1383493, whole genome shotgun sequence.
ACCESSION   DASTKU010000227 DASTKU010000000
VERSION     DASTKU010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383493"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="VFQ61_08470"
     CDS             <1..1445
                     /locus_tag="VFQ61_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954525.1"
                     /translation="LEGSDMSLVHLWEAPAGSTWPSVTRLASSTLPANIVGQNIITYG
                     RGMTTYATPPTGTTSGAGTWKSLSRTVASTRAFLGGTELIINAAANGNSIVGWGDSGS
                     GSLLNNNIVSVTTSLISYECYDSSTSDRCKQTTTKISSAGLRSVSDFATYINEAPNRP
                     LTWASPLTNIQSPWIQDRRLEYSSPDSNVVTLRGQLRREVQGTSKLFLLPSGLRPARR
                     MYVPVPALSGEILSARLRIEADGWVYLQTADATPNQVSVHLDGVQFSVKTDGFEALDL
                     ANGWVPFGNTMFTPAARKVGNTVYLQGSLKHPSTPSSMIVFTLDAGLRPPADTYVPIV
                     LCSSERGRLVIQPNGNVRIETENSDADPACLTSLDGVSFRISTAGTSAAAMQNGWTGG
                     VFSTLPLSFVFDGPWVRFLGAVGNGTDSHIITLPAKYRPRSTQVLTADGCGAQKAWVT
                     ITTDGKVRAYSATNGLPSCFVSFSGVSFVP"
     gene            complement(1553..2386)
                     /locus_tag="VFQ61_08475"
     CDS             complement(1553..2386)
                     /locus_tag="VFQ61_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9954526.1"
                     /translation="MNILVTGATGKVGSRLVKRLAQRGDHVRALVRDPARAAEIRGEG
                     VELVKGDLLDADSLGAAVRGVEAIVHCAAFFRGATAEQANAVNNVGTQHLANAARGAA
                     SVKRFIFTSTGLVYGTTDGRVSREDDPCSPTAAYPVSKLAAERLLFSIEGLDVRVLRL
                     PFVYGDGDPHIAEVIPMMRGFSPTQRMSIGHHVDVAQAVMRLLDAPSPAHRIYNVADD
                     EAPDLATLFASVGEPPPDGSDIERGLIFQAIMDGRRIREDLGFKPKYPRLADAVAASV
                     A"
     gene            complement(2461..2913)
                     /locus_tag="VFQ61_08480"
     CDS             complement(2461..2913)
                     /locus_tag="VFQ61_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954527.1"
                     /translation="MTLSESARLLRGGPALDSVRVEKNWARAQFFAWSATYGEDEFTW
                     TDLRQLERSNIEEAIYERPDESALREALAKDEAILLQRLQQLQQVQKLDILDDVYADL
                     RNCLAQRAVFGTGEGFFEELFRVYASGGWPCGWEGPYPDSGKLIAYYP"
     gene            complement(2956..>5412)
                     /locus_tag="VFQ61_08485"
     CDS             complement(2956..>5412)
                     /locus_tag="VFQ61_08485"
                     /inference="COORDINATES: protein motif:HMM:NF025769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HET9954528.1"
                     /translation="DAQGNPTLVLGPDGSGTRFRYNQLGWPVEILDARGNVQRNAFDL
                     RGKLRRVEEADGNVRDFEYDGEGRTEHVADRHHEILMTYSGMGRLATRTQAGTTVRFE
                     YDTEEQLRTITNEHGVVYGFERGPLGEVTLEHGFDGVRRAYEYDPLGRVQRIERANGL
                     QTHFDYDVLDRVVAVRHSDGTREAFAYGRDGMLARAEKGSTRVVFERDALGRVLREHQ
                     GDFWVQSEYDALGRRIRVASSLGLDQHVERNAAGDVIRIRATTSKDRHEASAQAAQRG
                     APRADEFEVSFTRDALGLELDRSLGGGVRSRWLRDKLGRPLQHEILIGTQRHRGVGYN
                     WDVGGRLSRIIDALSGPVAFSHDMLGQLAGTTHADGRVELRLPDAVGNLFKRSDRADR
                     RYGPAGQLLESIDVRGRITRYGYDAEGNLIEKRLQGGAVWRYAWGADGYLREVTRPDG
                     TRVVFQYDALGRRVLKSYRGQHTRWIWDGDVPVHEWVEGELEPAIVGSNAEPRDSDDA
                     RVNRREAELSRHLVRGPPERGSRDQPITWLFEPDTFAPLARLQAGLRQNIVTDQLGSP
                     IAMLNEQGEVTWSGELDTWGELRMSLGEAHACPFRYPGQYADPETGLHYNRFRYYDPE
                     SGQYASQDPIGLEGGLALHAYAPDPLVSADPLGLSTYGLTPGTGAPYRRLVPGTPGRV
                     RGGSSTQLGRNMFHAMGMARNTRRAGYQAQHVIPSELRNHPVLRALGMDLDDASNGIF
                     LPVPRGDVSPLSRHRGYHSVYNDFVRTRLDLINPADPLPSLDQQVAAVRDRLRYLQRQ
                     GLPLYPSQGASIALWTRMYR"
BASE COUNT          985 a         1764 c         1646 g         1017 t
ORIGIN      
        1 tgctcgaagg ctcggacatg tccttggtac atctgtggga agcccccgcc gggtccacgt
       61 ggcccagcgt gacgcgattg gcatcctcga ccttgcctgc gaacatcgtg ggtcagaaca
      121 tcatcaccta cggccgcggc atgaccactt acgccacgcc gccaaccggc accacctcgg
      181 gtgccggaac ctggaagtct ctgtccagga cggtggcatc gactcgagcg ttcctgggtg
      241 gaaccgagct catcatcaac gccgccgcca atggcaatag cattgtcggt tggggcgatt
      301 ctggctccgg cagtcttctg aacaacaaca tcgtttcggt caccaccagc ctcatcagct
      361 acgaatgcta cgactcctcg acctcggacc gttgcaaaca aacgaccact aagatcagca
      421 gtgccgggct ccgaagcgtc tctgatttcg cgacttacat caacgaagca ccgaatcgcc
      481 ctctaacctg ggcgagcccc ttgaccaata tccagagccc ttggatccag gatcgccggc
      541 tcgagtactc gtcaccggac tccaatgtcg tcacgcttcg cggccaactt cggcgcgagg
      601 tgcagggaac ttccaagctc ttcctcttgc cgtctgggct gcggcccgcg cgtcgcatgt
      661 acgttccggt tccggcgcta tcgggtgaaa tcctgagcgc gcggctgcgt atcgaggcgg
      721 acggctgggt gtacctgcaa accgcggatg cgaccccgaa ccaagtgtcg gtgcacctgg
      781 atggtgttca attctcggtc aagacggacg gcttcgaagc gctcgacttg gccaatggct
      841 gggtgccctt cggtaatacg atgttcacgc ccgccgcgcg taaggtcggc aatacggtgt
      901 acttgcaggg cagtctgaag catccgtcga caccgagttc gatgatcgtc ttcacgctgg
      961 acgcaggctt gcggcctccg gccgatacct atgttcccat cgtactctgc agctcggagc
     1021 gggggcgctt ggtcatccaa cccaatggaa acgtcagaat cgagacggag aatagcgacg
     1081 ccgatccggc ttgtttgaca tccctggatg gggtgagctt ccggatcagc acggccggaa
     1141 cgagcgcggc ggccatgcaa aatggctgga cgggaggcgt cttctcgacc ctgccgttgt
     1201 cgttcgtgtt cgacggacct tgggtgcgct ttctgggtgc ggtcggtaac ggtacggact
     1261 cgcacatcat cacgcttcca gcaaaatatc gcccgcgctc gacgcaggtg ctgacggccg
     1321 acggttgcgg agcccaaaaa gcatgggtga cgatcacgac tgacggcaag gttcgcgctt
     1381 acagtgcaac gaatggcctc ccgagctgct tcgtctcctt ctctggagtg tctttcgtcc
     1441 cgtaattggc gcaagcggtc cgtgtggctc tcggctgatg tttgcagtgc gtgccatcag
     1501 ccgaggccgt accccgtcac gtgaggtgaa agacatctcc tcaacgtatg cgctaggcga
     1561 cgctcgcagc caccgcgtcg gctagcctcg gatactttgg cttgaagccg agatcctcgc
     1621 gaatgcgacg accgtccatg atggcctgga agatgaggcc tcgttcgata tccgaaccat
     1681 ccgggggcgg ctcgcctacg gacgcgaaga gcgtggcgag gtcgggggct tcgtcgtcag
     1741 ccacgttgta gatgcgatgt gctggcgacg gcgcatcgag caagcgcatg acggcctgcg
     1801 cgacatccac gtgatggcca atcgacatgc gctgagtcgg cgagaagccg cgcatcatcg
     1861 gaatgacctc tgcgatgtgc ggatctccgt cgccgtacac gaaaggtagt cgaaggacgc
     1921 gcacatcgag cccctcgatc gagaagagca agcgctccgc ggcgagcttg ctgacggggt
     1981 acgccgccgt cggtgaacat gggtcatcct cgcgcgaaac acggccgtcc gtggtgccgt
     2041 aaaccaaccc cgtgctcgtg aaaatgaacc gcttcacgga ggcggcgccg cgggccgcgt
     2101 tcgcgagatg ctgggttccg acattgttca ccgcgttcgc ttgctcggcg gttgcaccgc
     2161 ggaagaacgc cgcacaatgt acgatggctt cgacccccct caccgccgca cccagcgaat
     2221 cggcatccaa taggtcccct ttcacgagct cgacaccctc tccgcggatc tccgcggccc
     2281 gcgcaggatc gcgaaccagc gcccgcacgt gatcgccacg ctgggcgagg cgcttgacga
     2341 gccgacttcc aacctttcca gtcgctccgg tcaccagaat attcatgtga ctcagcgtaa
     2401 gggccccgcc cctccgcggc ttggaagaac cggacaatct cgaacccggg cgcgtccgcg
     2461 ctacggataa taggcgatca gcttgccact gtcggggtaa gggccctccc agccgcaagg
     2521 ccatccccca gaagcgtaca ctcgaaacaa ttcttcgaag aagccttcgc cggtgccgaa
     2581 caccgcgcgc tgagcgaggc agtttcgaag atcagcgtaa acgtcgtcca agatatctag
     2641 cttctgaacc tgttgcagct gctgcaggcg ctgtaacaga atggcttcgt ctttcgcgag
     2701 cgcctcgcgc aacgctgatt catcgggtcg ctcgtaaatg gcctcttcga tgttcgaacg
     2761 ttcgagttgc cgcaggtccg tccaggtgaa ttcgtcctca ccgtaagtcg cgctccacgc
     2821 aaagaactgc gcccgagccc aattcttttc gactcgtacg gagtccagcg ccggccctcc
     2881 tcgcaggagg cgagcggatt cagagagcgt catgacttct tcgaccgcag ttcgatgggc
     2941 accatacgcc atggttcacc tatacattcg agtccaaagg gcaatcgagg cgccctgtga
     3001 cgggtagagc ggcaagccct gccgctgcag ataccgcagc cgatcgcgca cggcggccac
     3061 ctgctgatcc aatgacggca agggatccgc cgggttgatg agatctaggc gcgtccgcac
     3121 gaagtcgttg tatacggagt gatacccgcg gtgtcgggag agcgggctga cgtcgccgcg
     3181 cggtacgggg aggaagatgc cgttggacgc gtcgtcgagg tccatgccca gggcgcgcag
     3241 caccgggtga ttgcgcagtt cgctcgggat gacgtgttgg gcctggtagc ctgcgcggcg
     3301 ggtatttcga gccatcccca tcgcgtggaa catgttccga ccgagttgag tggagctacc
     3361 gcctcggact cgacccgggg ttcccggtac caatctgcgg taaggggctc cggttcccgg
     3421 tgtcagaccg taggtcgaga gtccaagggg gtcggcgcta acgaggggat ccggcgcata
     3481 ggcatgcagt gccaacccgc cctcgagccc gatcggatcc tgactcgcgt attgccccga
     3541 ctccggatcg taatagcgaa accggttgta atggaggccg gtctcgggat ctgcgtattg
     3601 accaggatat cggaacgggc aggcgtgggc ctctcccaaa gacatcctga gttctcccca
     3661 ggtgtcgagt tctcccgacc acgtgacttc gccctgctcg ttcagcatcg cgatcggcga
     3721 gccgagttga tccgtgacga tgttctggcg gagaccagcc tggagccgag ccagcggcgc
     3781 aaaagtgtcc ggctcgaaca gccaggtaat gggctgatca cggctgccgc gctctggcgg
     3841 cccccgcacc agatgccggc tcagctctgc ttcgcgacga ttgactcggg catcgtcgct
     3901 gtcgcgaggt tccgcattcg agccgacgat agccggctcg agctcgcctt ccacccactc
     3961 gtgcacggga acatccccgt cccaaatcca gcgggtgtgt tgccctcgat aggacttgag
     4021 cacgcggcgg ccgagtgcgt cgtactggaa gaccacccga gttccgtcgg gacgcgtcac
     4081 ttcgcgcagg taaccgtctg ctccccaggc gtagcgccac accgcgccgc cctgcagacg
     4141 cttctcgatc agattgccct ctgcgtcgta gccgtaacgc gtgatgcggc cgcgcacgtc
     4201 gatggactcg agcaattgac ctgcagggcc atagcggcgg tcggctcgat cggagcgctt
     4261 gaacagattg cctactgcgt ccggtaagcg gagctcgacc cgtccgtcgg cgtgggtcgt
     4321 ccctgcgagt tggcccagca tgtcatgact gaacgcaacc gggccgctta gagcgtcgat
     4381 gatgcggctc aagcggccac cgacgtccca gttatatcca acgccgcgat gccgctgggt
     4441 tccaatcagg atctcatgct gcagcgggcg accgagtttg tcgcgcagcc aacggcttcg
     4501 cacgccgcct ccgaggcttc gatcgagctc gagcccgagc gcgtcccggg tgaaggaaac
     4561 ctcgaactca tcggctcgag gtgcaccccg ctgagcagct tgcgcggacg cctcgtgcct
     4621 atccttcgag gtcgtcgcgc gaattcggat cacgtcccca gccgcattgc gttcgacgtg
     4681 ttgatcgaga cccagcgaac tcgccacccg aatgcgccgc ccgagcgcgt catactcgct
     4741 ctgcacccag aaatccccct ggtgttctcg cagcacgcgg cccaacgcat cacgctcgaa
     4801 gaccacgcgc gtcgagccct tctcggcgcg cgccaacata ccgtcacgcc catacgcgaa
     4861 cgcttcccga gtgccgtccg agtgtcgaac ggcgactacg cggtccagca cgtcgtagtc
     4921 gaaatgagtc tgtaggccgt tcgcgcgctc gatgcgctgc actcgcccga gcggatcgta
     4981 ctcgtacgcg cggcgcactc cgtcgaagcc gtgctctagc gtgacctccc cgagtgggcc
     5041 gcgttcgaac ccgtaaacga ctccatgttc attggtgatg gtgcgcagtt gctcttcggt
     5101 gtcgtactcg aatcgcactg tcgtcccggc ttgagtgcgc gtcgcgagcc ggcccatccc
     5161 gctgtacgtc ataaggatct cgtggtgacg gtctgccacg tgctccgtgc ggccctcacc
     5221 gtcgtactcg aagtcgcgaa cgttgccatc ggcctcctcc acgcgccgga gcttgccccg
     5281 caaatcgaag gcattccgtt gaacattgcc gcgggcatcc agaatctcga cgggccaccc
     5341 gagctggttg tagcgaaacc gagttccgct gccgtcggga cccagcacca gagtcggatt
     5401 cccttgcgcg tc
//
LOCUS       DASTKU010000228         6591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1607629, whole genome shotgun sequence.
ACCESSION   DASTKU010000228 DASTKU010000000
VERSION     DASTKU010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607629"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFQ61_08490"
     CDS             <1..269
                     /locus_tag="VFQ61_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954529.1"
                     /translation="VDRFPPPGWRNGDYGHGFGPDVFRGAKLACPAPVVVQGTPAPLP
                     SAEKARSELSDSSFLYQEQSDAVSLSSVRRLVPSVHAIPGCGCM"
     gene            353..1102
                     /locus_tag="VFQ61_08495"
     CDS             353..1102
                     /locus_tag="VFQ61_08495"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HET9954530.1"
                     /translation="MGHSATWLGIIALLAAAAAAFILLRFLIRKPALDFKQKLTLLIG
                     LGVLPVVAAASSTVRGMEATTHRDFCGSCHVMTAHYENAVAPSSQSLSARHSRNPYFG
                     EHSCYVCHADYGMLGYVLTKAGGLRHVYMYYLGGYRSMPLEEAKREIHLIKPYDNLNC
                     RQCHTTTLEDWRKVPEHAALSSELHLDRIACASAGCHGYAHPFNKPAEPKPGEPPVGA
                     RAGETGRSPPRDGSPPNHLQTGKPSEPKHAP"
     gene            1092..1352
                     /locus_tag="VFQ61_08500"
     CDS             1092..1352
                     /locus_tag="VFQ61_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954531.1"
                     /translation="MPRESKPWWSGLLAQSVTNLARVACMLALLALALMSYSIVSPRP
                     LPVILAMSLGHVLGGAAFCLYLSAVLLDVWRNSRSTPRERPE"
     gene            1445..2890
                     /locus_tag="VFQ61_08505"
     CDS             1445..2890
                     /locus_tag="VFQ61_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HET9954532.1"
                     /translation="MISKTAHYGLMLTIIGVWLGSCTGEDGKDGAPGLTGARGADGPQ
                     GPQGPSGLPGPAGGAGPEGPEGPEGPQGPAGPRGPAGPPGSVVVVGAGGGDGEGGATS
                     IPALTTSCLAPCHSFSGLVEQWKTSTHYSTYIANLGGEEVPTWTGNAACGNCHAIDGI
                     EQRVAGNITYSGTQPPNSAHGQTNSLNSTNNKVVESNYAGHATVAVVHCSTCHETNEG
                     TDPHLTGQNYTPGSFPLRVPSGPGDEGLLEKSSAVGVSDGTPATDYNRGNVCIWCHKS
                     RKDVTNYIAPTNNKLSSAYWGPHEGPQSDVYTGKGGYHFPGKTYGNSSHQAFENGCLD
                     CHMPRDANKNAGIGDHSFYPRLSSCQKAGCHANAKNFDVIGGQSAMRASIQELRVILN
                     NRMWLTRSSAAPYEPLTDTQLADQHFEEDVVLPNGALLTGDEAGALYNYLLLARGSGG
                     GIHNPVYVRELIYDSIMALTGNRPASIPARP"
     gene            complement(3160..4146)
                     /locus_tag="VFQ61_08510"
     CDS             complement(3160..4146)
                     /locus_tag="VFQ61_08510"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET9954533.1"
                     /translation="MLRGEFFAAARSSAVADAPVGCTIEDQRLVIFRDEQGVPRALAD
                     HCVHRGVPLSRGSVRNGCVECPYHGWRYDGAGRCVHIPDLQANKRIPTRAKVGSFRTL
                     ESDGFIWVQLEAARAPRWPLPLLQEVRGANWRAVSHSREVACDWRFMVENVLDGSHLP
                     FIHGGSLDKAGWFSFAWARGFRNGAAEAPPDLQVLENEAGFIASAESQLPDGGRRAFR
                     FRMLLPSTVCVELDRNSSQIRVWVHVVPTGPGRCRVEHVLHRNFLTFALADRLFLANA
                     RKILGEDARAVELQQRGYDRAGRDWEVSVLRDRLSLLFRRLVRSAGDPPAAV"
     gene            complement(4170..5219)
                     /locus_tag="VFQ61_08515"
     CDS             complement(4170..5219)
                     /locus_tag="VFQ61_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET9954534.1"
                     /translation="MTNETRDYSIDPGSADIERMHELASGQAVSASLYALVQLNLVDR
                     LLMGPRSALSLAKEIDANEDALYRLLRTVACLGVLEESGERVFELTPLGATLRADSPR
                     SLRPFVLMTGADWHREVWGELTHSVKTGSPSFVKIHGTDLFSFMHERRDVWQNFNETM
                     TSLSTIVNDVVPDVFDFSRFQRIVDIGGGHGSLIAAILRKNPEHVKGVLFDLPEVIRG
                     AEPHLARLGVRERCELIPGNFDAGIPLGGDVYIIKRFLHGFDDARCIATLDKLRGALE
                     PGGRVLVIEMVVAEPNRGLRSKVDDLEMLTLTRWGRERTLGEYEDLFRRAGLALMRAE
                     PLPLDMYVIEGSRAH"
     gene            complement(5336..>6591)
                     /locus_tag="VFQ61_08520"
     CDS             complement(5336..>6591)
                     /locus_tag="VFQ61_08520"
                     /inference="COORDINATES: protein motif:HMM:NF015706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HET9954535.1"
                     /translation="PPCQATDLHGRPVTRGPDPTGPLAALAFKVVHDPHAGQLTYVRV
                     YSGTLRTGESVLNSVRGGSERVQRLLRMHANKREQIKSLRAGDLAAVTGWQETATGDT
                     ACDAAAPVLLEKMAFPVPTMQVAIYPVDRMNREKLAAALGRLSREDPSVGVTVRSETG
                     ETLVSGMGELHLEILVDRLKREFRIDATIGRPRVAYRETIRHEASGEGKYMHHSMGKA
                     QYAHVKLRLAAAPRGAGVSFGATPTCGIPDEYIPAVDRGARALLEKGVLAGFPLIDVK
                     VELEGGSHNDVDSTVIAFQIAAALATFEAAQRAVPCLLEPILLVEAETPEEFTGTVVS
                     DLSQRRGRVTLVERRGPAEVVKAEVPVSTMFGYASDLRSLTQGRASFVAGFCRYSEVP
                     SSDVAGLIAQLGSDAQEDRCSRAAS"
BASE COUNT         1272 a         2141 c         2003 g         1175 t
ORIGIN      
        1 tagtcgaccg ctttccgccc ccgggttgga gaaacggtga ttacggccac ggtttcggcc
       61 cggacgtttt tcgaggggcg aaattggcgt gtcccgcgcc ggtcgttgtt caaggcaccc
      121 ctgctccgct accgagtgcc gagaaggccc gcagtgagct atcggattcg agttttttgt
      181 atcaagaaca aagcgacgcc gtcagcctga gctcggttcg gaggctggta ccttcggttc
      241 acgccattcc cggctgcgga tgcatgtaag tacgaaattc gacagtcatg atggtgaaca
      301 ttgccagtca ttcagttgtt gacccctgta tggaactgac tatcgctgta tcgtgggtca
      361 ttcagcgacc tggctcggaa tcatcgcgct gcttgcggcg gcggcggctg cattcattct
      421 actcagattt ttgattcgca aacccgccct cgacttcaaa cagaaactca cgctgttgat
      481 cgggctcggc gtactgcccg tggttgcggc ggcttcatcc accgtcaggg gcatggaagc
      541 cacgacacac agagattttt gtggctcctg ccacgtgatg accgcgcact acgagaacgc
      601 ggtcgcgccc agtagccagt ccctatccgc gcgacactcg cgaaatccct actttggcga
      661 acacagctgc tacgtctgcc acgcagacta cggcatgttg ggttacgtgc tgaccaaggc
      721 cggcggatta cggcatgtct acatgtacta tctcgggggc tatcggagca tgccgctgga
      781 agaagccaag cgcgagatac atctcatcaa gccctacgac aacttgaatt gtcgccaatg
      841 ccacacgact accctcgaag actggcgtaa ggtaccggaa cacgcagctt tatcgtcgga
      901 actgcacctc gaccgcattg cttgtgccag cgctggttgt cacggctacg ctcatccgtt
      961 caacaaacct gctgaaccca agccaggtga accgccggta ggcgcacgcg cgggggagac
     1021 cgggcgatcg ccgccccgcg atggctcgcc gccgaatcac ttacagactg gcaaaccctc
     1081 ggagccaaag catgccccgt gaatccaagc cttggtggag cggactgctt gcgcaatcgg
     1141 tcacgaattt ggcgcgagtg gcatgcatgc tcgcgttgct cgccttggcg ttgatgagct
     1201 actccatcgt gtccccgcgc ccgttgccgg tcattttggc catgagcctc ggacacgtgc
     1261 tcggcggggc ggcattctgc ctgtatttga gtgcggtgct gttggacgtt tggcgcaact
     1321 cgcgttcgac tccgcgtgaa cgcccggaat gaagtggcgc ttgccagtca gggttcgcgc
     1381 ctcgatcacc tttggaatgt ggagctctca ttggagaact tgagaccagg acggcggtga
     1441 agacatgata tccaaaacag ctcattacgg cctgatgctt acgatcatcg gagtatggct
     1501 cggcagctgc accggtgagg atgggaagga cggagccccc gggttgacgg gagcgcgagg
     1561 cgcagatggg ccacaaggtc cacaggggcc ctccgggttg ccggggcctg caggaggagc
     1621 tggtcctgaa ggacccgagg gacctgaagg cccacaggga cccgcgggac cgcgcggccc
     1681 cgcgggaccg ccgggcagcg tggtcgtggt gggtgcgggt ggtggcgacg gtgaaggtgg
     1741 cgcgacctcg attcccgcgc tgaccacgag ttgcttggcc ccctgtcaca gcttttccgg
     1801 cctcgtggaa cagtggaaaa cgagcactca ctactccacg tacattgcca atttgggtgg
     1861 ggaggaagta ccaacctgga cgggtaatgc tgcttgcggc aattgccacg cgatcgacgg
     1921 catcgagcag cgcgtggcgg gcaatatcac gtacagtggg acgcagccgc caaactcggc
     1981 gcacggccag actaactctt tgaactcgac gaacaacaag gtcgtcgagt cgaactacgc
     2041 gggccatgcc acggttgcgg tggtgcactg ctctacgtgc cacgaaacga acgaaggcac
     2101 cgatccgcac ctgaccggtc aaaactacac cccgggttcg ttcccgctgc gcgttccgtc
     2161 gggccctggt gacgaggggt tgctggagaa gagcagcgcg gtgggcgtat cggatggcac
     2221 acctgcgacc gactacaatc gcggtaatgt gtgtatttgg tgtcacaagt cccgcaagga
     2281 cgtaacgaac tacattgctc ctacgaacaa caagttgagc agcgcctact ggggcccgca
     2341 cgagggccct cagtccgacg tctacaccgg caaaggcgga taccacttcc cgggaaagac
     2401 ttacgggaac tcgtcgcacc aggcgttcga gaatgggtgc ctcgattgcc acatgccgcg
     2461 ggatgcgaac aagaacgcag gaataggcga ccactcgttc tatccgcgct tgagctcctg
     2521 ccagaaggcg ggatgccacg cgaatgccaa gaacttcgac gtgatcgggg ggcagagtgc
     2581 gatgcgggcc agcattcagg agttgcgcgt aatcctgaac aaccgcatgt ggctcacgcg
     2641 cagctccgct gccccttacg aaccactgac cgacacgcag ctagctgacc aacacttcga
     2701 agaagacgtc gttctcccga atggggccct gctgacgggg gacgaggccg gcgcgcttta
     2761 caactatctt ctgctcgcac gcggaagcgg tggcggcatc cacaatccgg tttacgtcag
     2821 agagctcatt tacgattcga tcatggctct gacgggtaac cgccccgcga gtatccccgc
     2881 gcgcccgtag tccgcgcgat cttcggcctc accgccaact ccgcgccatc gctgctcagc
     2941 acgcagcctc acccgggtca gtctcccgcc gactccaccc gggtgaggtt agagtgcgac
     3001 tctctcagcc cagcagccca gcctgcactg ccggcgaagg aggacggctg ggtctagaag
     3061 gctcgggtct agaaggctcg gaatgcccgc ttagcagagg ctctcggcgg cgcgccctac
     3121 gttgtccgga tcactctcgc cctctggcag cgcgagctct tacaccgcgg cgggaggatc
     3181 tccagcgctg cggacgagcc tgcgaaacag caaagaaagc cggtcccgca acacgctgac
     3241 ttcccagtct cgacccgcac gatcatagcc gcgctgctgc agctcgacgg cgcgcgcgtc
     3301 ctccccgagg attttcctcg cgttggccag gaacaggcga tctgcgagcg caaaggtcag
     3361 aaaattccgg tgcagcacgt gctctacgcg gcagcgccca gggccggtag gcaccacgtg
     3421 cacccagacc cgaatctggg acgagttcct atccaactcc acgcacacgg tgcttggcag
     3481 caacattcgg aatcgaaacg cgcgccgccc cccgtctggc aactgcgact cagcgctggc
     3541 aatgaagccg gcctcgttct ccagcacctg taagtcgggc ggggcctcgg cggcgccatt
     3601 gcgaaagccg cgcgcccagg cgaagctgaa ccaacccgct ttgtcgagcg acccgccgtg
     3661 gatgaatggc agatgagagc cgtccagcac attctcgacc atgaagcgcc aatcacacgc
     3721 cacttcacgg ctgtgcgaga ccgcgcgcca gttcgctcct cgtacttctt gcagcagcgg
     3781 cagcggccag cgcggagcgc gcgccgcttc gagctgcacc caaatgaacc cgtcggactc
     3841 caacgttcga aacgaaccta ctttggcacg cgtggggata cgcttgttgg cttgcagatc
     3901 gggtatgtgg acgcagcgcc cggccccgtc gtagcgccag ccatggtagg ggcactcgac
     3961 acatccgttg cgcacgctgc cccgggagag cggtacgccg cgatgcacgc agtgatccgc
     4021 cagcgcccgg ggtaccccct gctcgtccct gaagataacg agtcgttggt cttcaatcgt
     4081 gcacccgacc ggagcgtctg ccaccgcgct cgatcgggcg gccgcaaaga actcgccacg
     4141 aagcatgatt caatctcccg ctaacgccat cagtgggcgc ggctgccctc gattacgtac
     4201 atgtccaacg ggagcggctc cgcccgcatc aaggcgaggc ccgcgcgcct gaacaaatcc
     4261 tcgtattctc cgagcgtgcg ttcgcgcccc cagcgcgtca gcgtgagcat ctctagatca
     4321 tctaccttgc tacgcagccc acggttaggc tctgcgacga ccatttcgat caccaacacg
     4381 cgaccgcccg gttcgagcgc tccccgcagc ttatccaagg tcgcgataca gcgcgcgtcg
     4441 tcgaagccat gcagaaaccg cttgatgatg tagacgtcgc cgccaagagg aattccggcg
     4501 tcgaagtttc ccgggatgag ctcacatcgt tctctcacgc ccaggcgcgc gaggtgcggt
     4561 tccgcacctc ggatgacctc cgggagatcg aacaacacgc cctttacgtg ctccggattc
     4621 ttgcgcagga tggctgcgat caagctgccg tgacctccgc cgatatcgac gatgcgttgg
     4681 aatcgagaga agtcgaagac gtccggcacg acgtcgttga cgatggtcga gaggctcgtc
     4741 atcgtctcgt tgaagttttg ccatacgtcg cggcgctcgt gcatgaacga gaacaggtca
     4801 gtgccgtgga tcttgacgaa cgagggcgag cccgtcttga ccgagtgagt gagctcaccc
     4861 cacacctccc ggtgccagtc cgctcccgtc atcagcacga acggtcgcag agaacgcggg
     4921 gaatccgcgc gcagcgtcgc gccgagtgga gtcaactcga acacgcgctc gccggactcc
     4981 tcgaggacgc ccaaacaggc taccgtgcgc agcaagcgat aaagagcgtc ctcattggca
     5041 tcgatctcct tcgcgagcga gagcgcgctg cgcggcccca tcagcagccg atcgaccaga
     5101 ttgagctgaa caagcgcgta aagtgaagcc gataccgctt gccccgaggc cagctcatgc
     5161 atgcgttcga tgtctgccga tcccgggtcg attgaatagt ctcgagtctc gttcgtcatg
     5221 ggacctcgtg agtgggcggg cgaggcacga ccgatttctc ggtgcgctgc gaccatcaac
     5281 acggtctcaa cgaacggcag agcgcgttta ttacaggctc ccgcgggctc gcccctcagc
     5341 tcgctgcgcg cgagcagcga tcctcctggg cgtcggagcc gagctgcgcg attaaacccg
     5401 cgacgtcact ggagggtacc tccgagtagc gacaaaagcc cgcgacgaag ctggctcgac
     5461 cttgcgtcag agagcgcagg tcactcgcat aaccaaacat ggtcgagacc ggcacctcgg
     5521 ccttcaccac ctccgccggg ccacgacgtt cgactagagt cacgcggcca cgtcgttggc
     5581 tcagatcgct aacgacggta cccgtaaact cctccggggt ctctgcttcg accagaagaa
     5641 ttggctctag caagcaaggc accgcgcgtt gcgcggcctc gaatgtcgcg agagccgcgg
     5701 caatttgaaa cgcaatcacg gtcgagtcca cgtcgttgtg gctgccgccc tcgagctcga
     5761 ctttcacgtc gatcaaggga aatccggcca gcaccccctt ctcgagcaat gcgcgcgcgc
     5821 cgcggtcgac ggcggggatg tactcgtcgg gaatgccgca agtcggcgtg gccccaaagc
     5881 tcacgcccgc gccgcgcggc gcggcagcca ggcggagctt gacgtgagca tactgcgcct
     5941 tacccatgct gtggtgcatg tacttgcctt cgcccgacgc ctcgtggcga atggtctcgc
     6001 ggtaggcgac ccgcgggcgg ccaatcgtcg catcgattcg aaactcccgc tttagacgat
     6061 cgaccaagat ctcgaggtgc aactcgccca ttccggacac gagcgtctcc ccggtttccg
     6121 agcgcacggt gaccccaacg gacgggtcct cgcgcgacaa tcgccccagc gccgccgcca
     6181 acttctctcg attcatgcgg tcgacgggat agattgccac ctgcatcgtc ggtaccggaa
     6241 aggccatctt ctcgagcagc acgggcgcgg cggcgtcgca ggccgtgtcg cccgtcgccg
     6301 tctcctgcca gcccgtgacg gcggcgagat cgccggcccg caagctcttg atttgctcac
     6361 gcttgttggc gtgcattcgc agcagccgct gtactcgctc ggagccgcct cgcaccgaat
     6421 tcaatacgga ctcgcccgtg cgcaaggttc ccgagtacac ccgcacgtac gtgagctgcc
     6481 ccgcgtgagg gtcatgcacg accttgaacg cgagcgccgc caggggaccc gtcgggtccg
     6541 gtcctctcgt cacggggcgc ccatgaagat ccgtggcttg gcagggcggc a
//
LOCUS       DASTKU010000229        10633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1607636, whole genome shotgun sequence.
ACCESSION   DASTKU010000229 DASTKU010000000
VERSION     DASTKU010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10633
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607636"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFQ61_08525"
     CDS             <1..353
                     /locus_tag="VFQ61_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9954536.1"
                     /translation="FSDQLLDALIQFSMARGVSHAARIARGTPVLNSPPERVLSLRGP
                     VRALPRSPDAATLLPPLGAEAPAPSQEFLSTREAAALLGVTVKGLEAMRAKGRGPKFT
                     RVGKVVRYRRTDLV"
     gene            complement(422..1393)
                     /locus_tag="VFQ61_08530"
     CDS             complement(422..1393)
                     /locus_tag="VFQ61_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954537.1"
                     /translation="MNTLTDMRVDDLQHQADLGFGYRLAPPDGAVNLDSWKPYIGQFE
                     FDSMHDANTWVWVEGESGADLRPKIGHFETALLLTRAPQIRGAWMLEGPMENGVPRVA
                     SFGRRGERTIKEPPYPLTTATLRRAAALANNLTRTFELKALVGRLDRGFVSLVLAMSA
                     EYVEDSVLYLMRALEGMLCADNSREFKARAAAILRDGSAELLDELYVVRNKFTHAEPV
                     SKAFPAGMTEAQMTARCRQLQAFLYHFATRAYREVLSRPELMNRLTQPGVGAYWDKVI
                     EGTETLSFVVVVPDDVWNFEHDDHGTHYLMRHEAVIAELARDASTGA"
     gene            complement(1523..2074)
                     /locus_tag="VFQ61_08535"
     CDS             complement(1523..2074)
                     /locus_tag="VFQ61_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954538.1"
                     /translation="MRGTSDPGGGVCDAQVGSVPWAQRWRLEVQGICHNVPREPERLA
                     LYWGIGEQHEVWRLLNRPDGSTFNSLEELCEERYPWGFGRSREHMEVWMAIFNRRELP
                     RTPDNELRGLLHEAGEAMSRLGVYLRNARQSKANETNIELVSRAQRRLYEALRLLGER
                     AAVDLVNTLAADEPDRPPHPPIE"
     gene            complement(2071..2685)
                     /locus_tag="VFQ61_08540"
     CDS             complement(2071..2685)
                     /locus_tag="VFQ61_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954539.1"
                     /translation="MSRARAFVRPEIAARLDAVGVKVKATAIDERPVRRVSDLVGCHV
                     LEARWLLRRFVGFGDKSLAAVVEIAADLQERYLPAFEEMYGAPKIESSHIVWPTREGR
                     WRLRWDEQLEYMLRLECDRLDRVDAAATRNLRLHGRLGENLREFAKLEGISVDDAPGN
                     LERYRTVTRGKGDLEGLAVWGDPGDAEASTQAPTTPLNAPGGAA"
     gene            3234..3542
                     /locus_tag="VFQ61_08545"
     CDS             3234..3542
                     /locus_tag="VFQ61_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954540.1"
                     /translation="MSESNNIVLFPLPHGVEHDVPADDRRSEEQELQQILRRLTTDMS
                     ALAKVKALPRAKVLYESLHPETRHGGDRITAKQVSNSRTWSTFTKHVADTCGISQSTV
                     "
     gene            3515..4216
                     /locus_tag="VFQ61_08550"
     CDS             3515..4216
                     /locus_tag="VFQ61_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954541.1"
                     /translation="MRNLAVDRLTVYTHLRWAERLATLTPEAECEANATALANQLGTL
                     ARIAALPDPMIQRDLALRFDRQRTQVKKELAEREREFGLPTPSRSSKKAREPIDAEQE
                     VNTASDDSENGVAGGVEEENHADLVSGERSKSFDDARAQEPGLAAIEAMLGASTLDGC
                     LDAIRGLQGDRSEIEVLRSKLKHVEAKCAKLESHLTLYERAPLGELFRTIGVTNVHDA
                     IERTRALVGVSNAAE"
     gene            4203..5090
                     /locus_tag="VFQ61_08555"
     CDS             4203..5090
                     /locus_tag="VFQ61_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954542.1"
                     /translation="MPPSDRYERQLGFWWNRVNTRVFGGALLASPRILLDRALEVNAL
                     WDPTEATIRVSRQFVRAKSWWCVVEVLKYEMAHQYVSDVIGARDESERREVHQMVCER
                     FGLCGVPPRDACDAGLDAHLQELKALLAGGDPASDAAAMTLAKSIVPAPDTLLEGTKA
                     FSARAVGPASITVEQHERSLARFLVRLLGVECRWVGSLDPAGHDAQQLEVMGRLPHVR
                     AAERLQSVILEEAGRRWTMRGAGRGGNWLRFMVTMIDGFERAVPGTRPANGTVETRDA
                     ELIDFCSRRHRGDERPGPT"
     gene            5465..5671
                     /locus_tag="VFQ61_08560"
     CDS             5465..5671
                     /locus_tag="VFQ61_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954543.1"
                     /translation="MPAVRVAIAPNQTILDLLDQEWPLTVPKHRLMRIALEAGLRALA
                     DLDEEAFRHVVTKDAVASAGIRER"
     gene            5726..8779
                     /locus_tag="VFQ61_08565"
     CDS             5726..8779
                     /locus_tag="VFQ61_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HET9954544.1"
                     /translation="MSKRERTPEITEPQPNTTTLTAGTLLSTDEYLWKTSLEAARSIG
                     PDTVEAALDRYLDFLDHDDANVYELSAFDVENKDAIKDAGGWRRGANLIAYAKGRDEV
                     QRLTRLGLDFGAEALYLVSGKSIPTVGMAYKGRNAWYVMETHTTDEQILERRVLPFDL
                     DPAREPRERSATDDEHRLALRAGKLLTCYLWSLLDRLGKSRTCLAYGPSGNGFHCLLA
                     VSILEAPEVSGLYTSILDAARRRVRAWIHEGVDVDLTLSNASRLLPIYGTKKCKGGEY
                     PDEKIRHRWSYLAAQRPTERLTLDDLRALLEVLLEGLPAEAELKTGAPTAPAGPSPVT
                     TDDWQAYRNAIDAIPMAKVLEHVGLLDDSGKPICPGHGEPAAHGTDVAIISSRDGRVE
                     LLNCKHARCVGKGNCKSTKGTNWTGVGLVMNRRDCDVRTARDILGKAFDVPKPKGRPP
                     EDPATTDELLRMADGAGTEDGSAPEEGAAQTEPEASEPETPKPRAAFSGRRRACTDQG
                     NAERVFDLFGAQVGYLDGGTWLVWDGKRFERYKNDVKVQALVKRANRSIYLEAAQTEG
                     NARRRLQNWAKKSEGARSIAASIRLLRDLVLLDEDDLDTHPELLNCQNGVVCLKTGAL
                     LPHDPKYKLTQITRGNYDPSKPSNLMIAHLERCQPDPEVRAYIWRSAGYQITGLIREH
                     YYEIHTGSGKDDEEGRNGKGTFYNAISWALGDYAGAPPRDLLAQKPGEFDKHPTGFMT
                     VKKKRLCVTSEFERGQVLNVANGKILTGGDPIVGRGMRQDFGDPFWPTHKLVLVTNWL
                     IRIPDTDAAIWERVRVIRWPVYLQREARVLDALDKLRTQEEVDGIITLLVQGCLEWQR
                     AGQKTSPPQSVLDDTQKYRDAENEFGQFVDEHLAKTPGGKVNRKHVYERYKAWCAESG
                     KKPLSAGNFAKELVKRKVVEVGPGDKSGNERFWLGVTLVDDVKARFDADSAENERQLR
                     AAGYLPTEAANETAAASVAADDDLDLEAYVEGELARANGQDAAWN"
     gene            9355..>10633
                     /locus_tag="VFQ61_08570"
     CDS             9355..>10633
                     /locus_tag="VFQ61_08570"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HET9954545.1"
                     /translation="MAIRSTVGRLIGCLPQKVNANQSHLESVSIGEVDYIDYISDTFP
                     LHNTFCPYVHKRREFRHEQEVRAVLIITDAAAADSKAGAATGFRVTEMCLLLTIDMTP
                     ASARRRSGTSASAAKRRLYHLSTRPDMSDPVEDAYDDHDEPFTVTEYLDTAAELIDAL
                     RPTNARWGGQSLRWIFRGHANAGWELVPSALRKSAWRDFKPEYDPEVLQQAERDLVEW
                     EILKEFGDRLDRAGLPIPGKTRGELDRQDPRKLPANWVLLYVQLAALAQHHGLPTRLL
                     DFTRSGLIAAYFAALEPPDGPPERFCVWAIDGSFFTLGHGYEDQGTGLALVRGSRAFN
                     VNQHAQDGLLGMWETPSSLGDGKGGVSLLELMDFIRLLAPYHAKKPVCFRQACMSRCR
                     ASGSVSGSTRHDRSPQWDARRLACSTPASSLSAN"
BASE COUNT         1966 a         3429 c         3466 g         1772 t
ORIGIN      
        1 tgttctcgga ccagctgctc gatgcgctga tccaattctc gatggctcgt ggtgtctctc
       61 atgccgctcg catcgcacgc ggcaccccgg tcctcaactc accccctgaa cgggtactca
      121 gcctgcgtgg ccccgtgagg gcgctcccgc gctcaccaga cgccgcgact cttctcccgc
      181 cccttggcgc agaggctccg gctccgtcgc aggagttcct gtcgacgcgc gaggcggcgg
      241 cgctcctcgg ggtgaccgtg aagggcctcg aggcgatgcg cgccaaggga agagggccca
      301 agtttactcg agtcggcaag gtcgtccggt atcgccggac cgacctggtc tgagggacga
      361 cagacttccg cgctggaagc gggaccctga cgggtcccgc tttctggctg gcgcgcgcgc
      421 tttaggctcc ggtcgatgcg tcgcgcgcga gttctgcaat cacggcctcg tgccgcatga
      481 ggtagtgggt cccgtggtcg tcgtgctcga agttccacac gtcgtcgggt accacgacaa
      541 cgaaggacag cgtctctgtg ccctcgatga ccttgtccca gtacgcgccc acgccgggct
      601 gcgtcagtcg gttcatcaac tcgggccggc tcaagacctc tcgataggcc ctcgtcgcga
      661 agtggtatag gaaggcttgg agctggcggc aacgagccgt catctgcgcc tccgtcatgc
      721 cagcggggaa cgccttgctg acgggttcgg catgcgtgaa cttgttgcga acgacataga
      781 gttcgtcaag cagttcggcg ctgccgtctc gaagaatagc cgccgcgcgc gccttgaact
      841 cgcggctgtt gtccgcgcag agcatcccct cgagggcacg catcaggtac agaacggaat
      901 cctcgacgta ttcggcgctc atggcgagca ccaaggacac gaatccgcgg tcgagccgtc
      961 ctacgagagc cttcagctcg aaggtgcgcg tgagattatt cgcgagggcc gccgcccgcc
     1021 ggagcgtcgc ggttgtgagc ggatacggcg gctccttgat ggtcctctcg cctctcctcc
     1081 caaagcttgc gacacgcgga acgccgttct ccattgggcc ctctagcatc caagcaccac
     1141 ggatctgtgg cgcgcgcgtg agaaggagcg cggtctcgaa gtgtccgatc ttcggacgga
     1201 gatcagcacc tgattcgccc tcgacccaga cccaggtgtt ggcgtcgtgc attgaatcga
     1261 actcgaactg cccgatgtat ggcttccacg agtccaggtt gacggcaccg tcgggcggcg
     1321 cgagacgata cccgaagccg aggtctgctt ggtgttgcag gtcgtcaacc cgcatgtcgg
     1381 tcagcgtatt cattgccaag caggcgaatc gagtctcgct cacgtcccaa agcttaccac
     1441 gacgtgggca gagacacaga gcgcaaagag ggcccggctc gcactcgacg agccgggcgt
     1501 ttcaggcggg agccagcgag tgctactcga tgggcgggtg cggcggcctg tcgggctcgt
     1561 ccgcagcgag cgtgttcacg aggtcaaccg ctgccctttc cccgagaaga cgcagcgcct
     1621 catagaggcg cctctgcgct cgacttacga gttcgatgtt cgtttcgttc gccttggatt
     1681 ggcgcgcatt cctcaggtac acgccgagcc tagacatcgc ttcaccagct tcgtgcagca
     1741 gcccgcggag ttcgttgtct ggcgtacgcg gcagctcacg ccggttgaaa atcgccatcc
     1801 acacctccat atgttcgcgc gaccgcccga agccccacgg gtatcgctcc tcgcacagct
     1861 cctctaggga gttgaaggtg gatccgtctg gcctgttgag caggcgccac acctcgtgct
     1921 gctcgccgat cccccagtac agagccagcc gttccggctc gcgcggaacg ttgtggcaga
     1981 ttccctgaac ctcgaggcgc cagcgctgtg cccaagggac gctgcccacc tgcgcatcgc
     2041 atactcctcc gcccgggtcg gacgtgcccc tcacgccgca ccgcccgggg cgttcagggg
     2101 cgtcgttggc gcctgagttg acgcttctgc gtcgcccggg tcgccccaca ctgcgaggcc
     2161 ctccaggtcg cccttgccgc gcgtgactgt gcgatagcgc tccaggttgc cgggggcgtc
     2221 gtcgacggag attccctcga gcttggcgaa ctcccggagg ttctctccca gccgcccgtg
     2281 gaggcggagg ttgcgcgtgg cagccgcgtc gactcgatcg aggcggtcgc actcgagccg
     2341 cagcatgtac tcgagttgct cgtcccagcg caggcgccag cgtccctccc tcgtgggcca
     2401 gacgatgtgg ctgctctcta tcttgggcgc gccgtacatc tcctcgaacg cgggcaggta
     2461 ccgctcttgg aggtcggcgg cgatttccac caccgcggcc aacgacttgt cgccgaagcc
     2521 gacgaagcgg cggagcagcc accgcgcctc aagcacgtga cagccgacga ggtccgacac
     2581 ccgccgcacg ggtcgctcgt caatggccgt ggctttgacc ttcacgccga cggcgtcgag
     2641 cctcgccgct atctccgggc gcacgaacgc gcgggcgcgg ctcacgctgc cacctcctgc
     2701 tcggcaacga acgtttcgaa gaccggcggt gtcatgacat acgtgtagct ggcccgctgc
     2761 atctgctctg gcatatcgca tctcctctgc gggcgtcgta gcgcccgtga ggtcgcgtta
     2821 caccggcgca gagtctctcc cggtaagtcg atgtcgattt cggcagaggt cggaattgtg
     2881 gggctgacgc aacgtgccgg tgcttccaag gcttcgtctt gtgcttggat cggacccctc
     2941 tcgcgcatcc ctgcgacgcg cactttcact gttcagtttc gcctttacgt tgaacgtcgc
     3001 gcacgtcgcg cgagcgctgc ctatgctgaa cgtgctcgcc gggtcaccac tgacccgcaa
     3061 tcgcagcctt gacccgcagt gcgttctcgc agagcatcac gtctggggga cgcacgaagc
     3121 gttctcaaga acttcgtcaa cgctctcatc accggtgcga ctcggaccgc cgcgcgcggc
     3181 ccacccaggc atcggccacc gagcaggcca cgaacggccg gaggtgtgcc tttatgtccg
     3241 agtccaacaa cattgttctg tttcctctgc cgcacggtgt cgaacacgac gtgcccgccg
     3301 acgaccggcg aagcgaagag caagagctgc agcagattct ccgtcggcta accaccgaca
     3361 tgtcggcgct tgccaaggtc aaggcgctgc cacgcgcgaa ggtcctctac gagtcgctcc
     3421 atccagagac caggcacggc ggagatcgga tcaccgccaa gcaagtttca aattctagaa
     3481 cttggtcgac gttcaccaaa cacgtcgccg atacgtgcgg aatctcgcag tcgaccgtct
     3541 gaccgtctac acccacctcc ggtgggccga gcggctcgcg accctgaccc ctgaggccga
     3601 gtgcgaggcc aacgcgacgg cgctggccaa tcagctaggt actctcgcca ggattgcggc
     3661 ccttcctgat cccatgattc agcgcgacct ggcgcttcgg ttcgacagac agcgcaccca
     3721 ggtgaagaag gagcttgccg aacgcgagcg cgagttcggt ctgcccacac catctagatc
     3781 aagcaagaag gcgcgcgagc cgatcgacgc cgagcaggag gtcaacaccg cttcggacga
     3841 ctccgagaac ggtgtcgctg ggggcgtgga agaagagaac cacgctgacc tggtgagcgg
     3901 cgagcgttcc aagtccttcg acgatgcgcg cgctcaagag ccgggcttgg cggccatcga
     3961 ggctatgctc ggagcgtcga ctcttgatgg gtgcctcgac gccatacgcg gcctccaggg
     4021 agacaggtcc gagatcgagg tgctgcgcag caagttgaag cacgtggagg cgaagtgcgc
     4081 gaagctcgaa agccacctga cgctgtacga gcgcgcccct ctgggcgagc ttttcagaac
     4141 gattggcgtt acgaacgtcc atgacgccat agagcgaacg cgcgcactgg tgggagtcag
     4201 caatgccgcc gagtgaccgc tacgagcgcc agctcgggtt ctggtggaat cgggtcaaca
     4261 cgcgggtgtt cggcggcgcg ctcctcgctt ctccccggat cctgctcgac cgggccctcg
     4321 aggtgaacgc gctgtgggac ccgacagagg caaccatccg cgtatcgcgg cagttcgttc
     4381 gcgcaaaatc gtggtggtgc gtggtggaag tcctcaagta cgagatggcg catcagtacg
     4441 tgtccgacgt gatcggggcc cgcgacgaga gtgagcgccg ggaggtccac cagatggtct
     4501 gcgagcggtt cgggctgtgt ggcgtgccgc ctcgcgacgc ttgtgacgcg gggttggacg
     4561 cgcacctcca ggagctgaag gcgcttctcg cgggtggtga tccggcgagc gacgctgcgg
     4621 ccatgacgct cgccaagagt atcgtgccgg cgccagacac gcttctggag ggcacaaaag
     4681 ccttttcagc gcgcgcggtg gggccggcaa gtattacggt ggagcagcac gagcgttcac
     4741 tcgccaggtt tctcgttcga cttctcggcg tcgagtgccg ttgggtgggg tccctggatc
     4801 ctgccgggca tgacgcccaa cagctcgagg tgatggggcg cctcccgcat gttcgcgcgg
     4861 ctgagcgctt gcagtccgtg atccttgagg aggcagggcg gcgctggacc atgcgtggcg
     4921 caggtcgagg cgggaactgg ctcagattca tggttaccat gattgacgga ttcgaacgag
     4981 ccgtgccggg aacccgcccg gcgaatggaa ccgtggagac acgggacgcc gagctgatcg
     5041 atttttgctc aaggcgccac cgcggcgatg agcggccggg cccgacatga gtgaatcccg
     5101 tcgcacgtag tgatcaccgc gtattgcgga gagtgggcac caccagcccg tcgaccctgc
     5161 gcgcgcgtgg tgaacgtcca ggggacgagc gcaccagctc accagcgcgg tcgccgcccc
     5221 cgtgtggggc gcgcttcccc ccagaaggcg catcagatcg gaaaggaact cccacatcgg
     5281 tcgtgcctgc tcgtgcgccc gtacggatgt tgtgagcaac ggaccccgac tgcaggcggc
     5341 gacgccgaag ctaggaggtc cagccaaccc cttgccgctt gtcgcggatc acgggtgatg
     5401 cgcccggtgt aacagcagat cgggatcgca gatctgttta tgcgaggcct ttcacggaga
     5461 aaaaatgcca gctgttcgcg tcgccatcgc gccgaatcaa accatccttg atcttctcga
     5521 tcaggaatgg ccgctcaccg tgcctaagca ccggctcatg cgcatcgctc tcgaagcggg
     5581 cctgcgcgcg ctcgccgacc tcgatgagga ggcatttcgg catgtcgtca caaaggacgc
     5641 tgtcgccagc gcggggatcc gcgaacggta aaaaaaacgc cgggcggcaa cccggcgtct
     5701 ccgccccgca ggagggggca cgaacatgtc caagagagag cgtacaccgg agatcaccga
     5761 accgcaaccc aacaccacca ctctgaccgc agggactctg ttgtcgaccg acgaatatct
     5821 atggaagacg agcctcgagg cggctcgcag catcggtccc gacaccgtag aggcagcgct
     5881 cgatcggtac ctcgactttc tcgatcatga cgacgcgaac gtgtacgagc tgagcgcgtt
     5941 tgacgttgag aacaaagacg ctatcaagga cgcgggcggc tggaggcggg gagcgaactt
     6001 gattgcctac gcgaagggcc gggacgaggt gcagcggctg acacgcctcg ggctcgactt
     6061 cggcgcggag gcgctctacc tcgtctcggg caagtccatc ccgacggtgg gcatggcgta
     6121 caaagggcgc aacgcctggt acgtcatgga gacgcatacg acggacgagc agatcctcga
     6181 gcggcgcgtg ctgccgttcg atctcgatcc cgcgcgtgag ccaagggagc gcagcgccac
     6241 ggacgacgag cacaggctcg ccttgcgcgc cgggaagttg ctgacgtgct acctgtggtc
     6301 gctgctcgat cggctcggga agagccgcac gtgtctcgcc tacggcccga gcggtaatgg
     6361 tttccactgc ctcttggcgg tttcgattct cgaggcaccc gaggtgtcgg ggctgtacac
     6421 atccatcctc gacgccgccc gtcgccgtgt ccgcgcctgg atccacgagg gcgtggacgt
     6481 cgatttgacg ctatcgaatg cgtcccgcct gttgccgatc tacgggacga agaagtgcaa
     6541 gggcggcgag tacccggacg agaaaatccg tcaccggtgg agttacctgg ctgcccagag
     6601 gcccaccgag aggctcacgc tcgacgacct tcgagcgctc ctcgaggttt tgctggaggg
     6661 gctccctgcg gaagccgagt tgaagacggg cgcgccgact gcgccagctg gcccatcccc
     6721 ggtaaccacc gacgattggc aggcataccg gaacgcgatc gatgccatcc cgatggcgaa
     6781 ggtgctcgag cacgtcggac tcctcgacga cagcgggaag ccgatctgtc cgggccacgg
     6841 tgagccagcc gctcacggca ctgatgtggc catcatctcg tcgcgcgacg gccgggtcga
     6901 actgctgaac tgcaagcacg cgcggtgcgt cgggaagggc aactgcaaga gtacgaaggg
     6961 cacgaactgg acgggtgtcg gcctcgtgat gaaccgtcgc gactgcgacg tgaggacggc
     7021 tcgggacatt ctgggcaagg cctttgacgt cccgaagccg aaggggcgcc cgccagagga
     7081 cccagcaacc accgacgagc tactccgcat ggccgacgga gctggcaccg aggacggcag
     7141 cgcgcccgaa gaaggagcag cccagacaga gcctgaagcg tcggagcccg agacgccgaa
     7201 gccgcgggcg gccttttcag gtcggaggag ggcctgcacg gaccaaggca acgcggagcg
     7261 ggtcttcgac ttgttcggcg cgcaggtggg gtacctcgac gggggcacct ggctcgtctg
     7321 ggacggcaag cggttcgagc gatacaagaa cgatgtcaag gtccaggccc tcgtgaagcg
     7381 cgcgaaccgg agcatctacc tcgaggccgc ccagaccgag gggaacgccc ggcggcgcct
     7441 acaaaactgg gcgaagaaaa gtgagggcgc tcgcagcatc gccgcgtcga ttcgactgct
     7501 acgcgacctg gtcctcttgg acgaggacga tctcgatacc cacccggagc tgctgaactg
     7561 ccagaacggc gtcgtctgtc tgaaaacggg cgcgctcctc ccgcacgacc caaagtacaa
     7621 gctgacgcag atcacgcgcg gtaactacga cccgtcgaag cccagcaacc taatgatcgc
     7681 gcacctcgag cgttgccagc ccgatcccga agtgcgggcc tacatctggc gatcggcggg
     7741 gtaccagatt acgggcctca tccgggagca ctactacgag attcacacgg gctccggcaa
     7801 ggacgacgag gaaggccgca acggcaaggg cacgttctac aacgccattt cgtgggcgct
     7861 cggagactac gcgggcgcgc caccaaggga tctgctcgct cagaaacccg gcgagttcga
     7921 caagcatccg acgggcttca tgaccgtgaa gaagaagcgc ctatgcgtca cgtccgagtt
     7981 cgagcggggt caggtcttga acgtcgcgaa cggcaagatt ctcaccggcg gcgatcccat
     8041 cgttggccgt ggtatgcggc aggacttcgg agatccgttc tggccgaccc acaagctggt
     8101 cctcgtcacc aactggttga tcaggatccc tgacacggac gcggcgatat gggagcgcgt
     8161 gcgcgtgatc cggtggcccg tctatctaca gcgtgaggcc cgagtgctcg acgcgctcga
     8221 caagcttcga acgcaggagg aggtcgacgg catcatcacc ctgctcgtcc agggttgcct
     8281 cgagtggcag cgcgcgggcc agaagacttc gccgccgcaa tccgtgctgg atgacaccca
     8341 aaagtatcgc gacgccgaga acgagttcgg gcagttcgtc gacgagcacc tggcgaagac
     8401 gccgggcggc aaggtcaacc gcaagcacgt gtacgagcgc tacaaggcgt ggtgcgccga
     8461 gagcggaaag aagccgctca gcgcggggaa cttcgccaag gagctggtga agcgcaaggt
     8521 ggtcgaggtg gggcccggcg acaagtccgg caacgagcgc ttctggctcg gggtgacgct
     8581 cgtcgatgac gtaaaagctc gcttcgacgc cgacagcgca gagaacgagc gccagctgcg
     8641 tgcggccggc tatttgccga ccgaagccgc caacgagacc gctgccgcgt cggtcgccgc
     8701 cgacgacgac ctcgacctcg aggcgtacgt cgagggggag ctggcgcgcg caaacgggca
     8761 ggacgccgcg tggaactgat tgccgtgatg gagggcacgt tagggcacgt cacagaacac
     8821 gtgccctcca tctaaccgtc aagatcttca agcactctca gccacaaagg gcacgagggc
     8881 acgtcccaac gttccggagc tggcagcatg atataattgg cccacgggtg cattattttg
     8941 aaagtcacgc tgatagattg gggacgtgcc ctcgtgcccg ccttggtccg agacccgtag
     9001 cgcacgctgg tgaaggcacc tggcgaggcg cgacagcctg cgccgggtgc cgtctcacac
     9061 tggcagcccc gcacgccagc gcccacgtgc ccgcacaaat ccacggcccc gcgccgtgaa
     9121 cgcgcgcgag ccgcgcatga cccgaccatg tcggccacga accctcgcta ctgctacgag
     9181 gacagggttc cgaccttcgg aaggggagca ggccggggct ggtcgcccga gtcgagcacg
     9241 gagtttcggg ttcagcgcag gcgggttcgt aacgacgtcg gggtcaacgg ctggcaccga
     9301 gccacccacg agacggaagg catgtgagtc cgctacggcg gggctggcga gtgcgtggcg
     9361 atccgctcta ccgtggggcg gctcatcggg tgcctgcctc agaaggtgaa cgccaaccag
     9421 agccacctcg agagcgtgtc gatcggggaa gtcgactaca ttgactacat cagtgatacg
     9481 ttcccactac acaacacgtt ctgcccctac gtacacaagc ggcgggagtt ccgccacgag
     9541 caggaggtcc gcgccgtgct catcatcacg gacgctgccg ccgcggactc gaaggcggga
     9601 gcggcgacgg ggttccgggt gaccgagatg tgtcttctcc tgaccatcga tatgacaccg
     9661 gcgagcgcgc gtcggcggag tggcacttcc gcgagcgcgg cgaagcgccg tctttaccat
     9721 ctgagcacac gacccgatat gtctgacccc gtcgaagacg cctacgacga ccacgatgag
     9781 ccatttaccg tcacggagta tctcgacaca gccgccgaac tgatcgacgc gctgcgaccc
     9841 accaacgcgc gttggggcgg ccaaagcctc cgatggatct tccgagggca cgcaaatgct
     9901 ggctgggaac tcgtgccgtc agcgctacgc aagagcgcgt ggcgggactt caaaccggag
     9961 tacgaccccg aggtcttgca gcaggcagaa cgagacctgg ttgaatggga gattctgaag
    10021 gagttcggag atcgactcga ccgagcggga ttacccatcc ccggcaaaac cagaggcgaa
    10081 ctcgaccgac aggaccctcg caagctgccc gcgaactggg tgcttctata cgtccagctt
    10141 gccgccctgg cacagcatca cggactgcca acacgcctct tggatttcac gcggtcgggt
    10201 ttgatcgcgg cttacttcgc ggcgttggag ccaccggacg ggcccccgga gcgtttctgc
    10261 gtttgggcga ttgatggttc cttcttcacg cttggccacg gctacgaaga tcaaggcacc
    10321 ggccttgccc ttgtgagggg ctcaagagcg ttcaatgtca accagcatgc tcaagatgga
    10381 cttctaggca tgtgggagac cccgtcaagt cttggagacg ggaagggcgg cgtgtcgctt
    10441 ctcgagctga tggacttcat ccgtttactg gctccctacc atgcgaagaa gccagtgtgc
    10501 ttccggcaag cgtgcatgag ccgttgccgc gcgtcgggta gcgtgagcgg gtccacccgc
    10561 cacgacagga gcccccagtg ggacgcaaga agactcgcct gctcgacccc cgcgtcgagc
    10621 ctctccgcaa aca
//
LOCUS       DASTKU010000230         5668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_492206, whole genome shotgun sequence.
ACCESSION   DASTKU010000230 DASTKU010000000
VERSION     DASTKU010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492206"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..2094)
                     /locus_tag="VFQ61_08575"
     CDS             complement(295..2094)
                     /locus_tag="VFQ61_08575"
                     /inference="COORDINATES: protein motif:HMM:NF028151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HET9954546.1"
                     /translation="MMKQLLRFAVLASVFAPASALAQSQPISTCQAVTYQAEAMFKST
                     GGAVAGGWNIWSNGYISTNHVFGGASTVITVRARGESALGVAPTMVVSVGGATVYTTS
                     VASTSWADYTFITSPSAGSREVRVSFTNDYYAGGQDRNLFVDSVQVGSSDGWTDLTLR
                     NGWTGAANSCAPGVKLINDTITYRGGLSGAAATSDIAFCLTPSLEGSPDYTAFQTVDL
                     GYVVVRAAMAGGATGSVIVQPPHPLDPFSSYCAQMNQDGSAEIGPAAKAFTSLEGVSY
                     DRTTNDAQGVDRDPEWEGSPYAWRGHDGTQHEGANVYAKLVDGFVRFQGIITPSNSST
                     TGALLLTLPDPAMFPDRPVSVPVVMAPSSDQIAGSVGINTNGEVVVLQSLSQALADGL
                     TGVSLDGVSFAVSSTGAQSISLANGWVQQNSRPVRARYDEGVVRLEGSVTAGDTPTIG
                     TLPAGLRPSKTVYVPVVSALFGLDSTLSIDPSGTITVIDPDAEMELIGISLEGVSFSA
                     PQPFQCTGGCLSATPLARNQNSGPFGTTGERWFVVNTSINGWQASEIQGRTITVNGVP
                     VTAGQMPLPAPVGGAYYFRFSAGANNWSSMSFW"
     gene            complement(2544..3368)
                     /locus_tag="VFQ61_08580"
     CDS             complement(2544..3368)
                     /locus_tag="VFQ61_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HET9954547.1"
                     /translation="MSRPDSLSCQGHYRQIGQRIGPAILLPSTIGIAARAPPEAGSRP
                     VPPHSPPPVEPLSDDEADRLADFLEAHSPFDIEGLLGVLNAVAVAPSLLPPSAWLPVV
                     LPAGLGGLGAAEAEEFFGLLLRQYGDVLDALEHQQAVMPRPEDVDECKAFAEGYAAGA
                     EIDPVWLGNVDHWSFAAPMAYLGGRLDLVSEKTAEDIQRNFAPEPEQILCRSMDGLLG
                     ATYKTFQEVRRNALSHTSAATPSSTSRAGRNDPCPCGSGKKYKRCCIDSGSEVAAS"
     gene            complement(3421..3612)
                     /locus_tag="VFQ61_08585"
     CDS             complement(3421..3612)
                     /locus_tag="VFQ61_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954548.1"
                     /translation="MPVLSGPMPRFSRTVDLVNHFPILADEPLEPGELRLRTNLAALE
                     LWGREHISLPEILWTGPNP"
     gene            3636..3935
                     /locus_tag="VFQ61_08590"
     CDS             3636..3935
                     /locus_tag="VFQ61_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954549.1"
                     /translation="MRNVSELDFDSVEVNEIAFGALQRGAQSGYRLATGCVYSYGAMK
                     VTSGKQRFATWPIDFVDERPLQAGYYTHALTVQPERQPGDAGGIDSTLSKDAAPR"
     gene            4311..4613
                     /locus_tag="VFQ61_08595"
     CDS             4311..4613
                     /locus_tag="VFQ61_08595"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET9954550.1"
                     /translation="MKLHKWNDIKRSQREPARAERVRKRVEQELIEMSLADLRRELGI
                     TQAEMANAADMAQPQVSALESREDHLVSTLRRCVRALGGELEVIAVVGDKRVKLAV"
     gene            4594..4983
                     /locus_tag="VFQ61_08600"
     CDS             4594..4983
                     /locus_tag="VFQ61_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954551.1"
                     /translation="MSSLPFDTEHETWVCTLCRGYECDFPGCDPVRDAEWLTRHWNTR
                     PSCRFDAAAGKLVPVDLSKTPCPSEVAEAALARWCAPEYRALLGRIAARDGLSSEMGR
                     TFARWDSPEYEDLLMAAFARAKQPGDG"
     gene            5364..5570
                     /locus_tag="VFQ61_08605"
     CDS             5364..5570
                     /locus_tag="VFQ61_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954552.1"
                     /translation="MSCPDVDPELHVVALVSRTFRGARTSDLHAWDWSHVDTAGWVER
                     TRAQAEDENQGFSGTRSLIHLAAR"
BASE COUNT         1019 a         1834 c         1867 g          948 t
ORIGIN      
        1 gggctgctcc aggagcagct ggctgctcct tgttcagggt cggtgcctgt cgaacagcag
       61 ctcagctgcg gcccagtcag gggtccggac attgaagctg gcacggcgcc gcgccacacg
      121 tcagttgcta ctcgctggat acgaggacgc gccccgcccc gtagcaccag cgccagggtc
      181 accagcgcca cgggcattac cctgcgacgc gcttccccac aaaaccacaa aggcaacgag
      241 tgcccagccc cccctgagct ggacactcgc agcatcactc acctctggtt caattcacca
      301 gaagctcatg ctcgaccagt tgttcgcgcc cgcactgaag cggaagtagt aagcgccgcc
      361 gacaggcgcc ggtagcggca tttgacccgc cgtgacgggc acgccgttga cggtgatggt
      421 gcggccctgg atctccgagg cctgccagcc gttgatgctg gtgttcacga cgaaccagcg
      481 ctcacccgtc gtgccaaacg ggcccgagtt ctgattccgc gccagcgggg tcgctgacag
      541 gcacccgccc gtgcactgaa agggctgcgg cgcactgaag gacacgccct ccaggctgat
      601 gccgatgagc tccatttcag cgtccggatc gatcaccgtg atggtaccgg aaggatcgat
      661 gctgagggtc gagtcgagcc cgaagagcgc actcacgaca ggaacgtaga ccgttttcga
      721 gggccgcagg ccggcgggga gagtgccgat cgtgggcgta tcgcccgccg taacggaccc
      781 ctccaaccga acgacgcctt cgtcgtagcg cgcccgcacg gggcgagaat tctgctggac
      841 ccaaccgttg gcgaggctga tcgattgagc ccccgtcgag gacacggcaa aagaaacgcc
      901 gtccaagctg acgcccgtaa gcccgtctgc gagtgcctgg ctcaacgact gcagtacgac
      961 cacctcgcca ttggtattga tgcccacact gccggcgatc tgatcgctcg atggcgccat
     1021 cacgacgggc acggacaccg gccgatccgg aaacattgcc ggatccggca gcgtgagcaa
     1081 cagggctccg gttgtggacg agttgctcgg agtgatgatc ccctgaaacc tcacgaaccc
     1141 gtcgacgagc tttgcgtaga cgttggcgcc ctcgtgttgg gtaccatcgt ggccgcgcca
     1201 cgcataaggc gacccctccc actcggggtc gcgatcgaca ccctgcgcgt cgttcgtcgt
     1261 gcggtcgtag ctgacgcctt cgagcgaagt gaacgccttt gcagcggggc caatctctgc
     1321 tgaaccgtcc tgattcatct gcgcgcagta gctggagaac gggtcgagcg ggtgcggtgg
     1381 ctggacgatg accgagccgg tggcaccccc tgccatcgct gcgcgcacaa ccacgtagcc
     1441 gagatcgacg gtctgaaacg ccgtgtagtc cgggctgccc tcgagcgaag gagtcagaca
     1501 gaaagcaatg tcactggtgg ctgctgcgcc gctcaatccg ccccggtacg tgatggtatc
     1561 gttgatgagc ttgacgcccg gcgcgcagct gttggccgcg ccggtccacc cgttgcggag
     1621 cgtgagatcc gtccagccat cgctggagcc gacttggacg ctgtcgacga acaggtttcg
     1681 atcctgacca cctgcatagt agtcgttggt gaagctcacc cgcacctcac gcgagcccgc
     1741 gctcggactg gtgatgaagg tgtagtcagc ccaggacgtg ctcgccacgg aggtggtgta
     1801 gaccgtggcg ccaccgacgc tcaccaccat ggtaggggca acgccgagag ccgactcacc
     1861 ccgggcacga acggtgatga ccgtcgatgc gccaccgaag acgtgattcg tgctgatgta
     1921 gccgttgctc cagatgttcc acccgccagc caccgccccg ccggtggact tgaacattgc
     1981 ctccgcctga taggtaactg cctgacaggt gctgatgggt tgtgactgcg ccagcgcgga
     2041 cgcgggcgca aagacggacg ccaatacagc aaagcgcaaa agctgcttca tcatccctcc
     2101 gatgtctcga ggccgcattc cgccgcggcc tactccgcaa gcgtgaacga cttcctacgg
     2161 tgttctctga cccgggctgc ttgcccggtc aagccacggt ccatgcacga tgaccgatgt
     2221 cgctcagcgt gtactgggtg caccacgacc acgcctttgt gacgcggcat tctcaacaca
     2281 ccagctggag ctcacggtcg atgcccttgg ggactccgtg tccgtcacgc tcacgagtcc
     2341 accgagggta ctcggcagcc tgccgcccaa ggtggaggcg caggcgctgg ccttcgtctc
     2401 caagaatcta ggggcgctga tcgagcactg gaatggagag catcgacacg ggcgaggtga
     2461 tcgagcggct tcagcgggtc tgacactgcg gcgctgccga gcacccgcaa gtcgaaggct
     2521 gacggcccgg accgagcatc gggctacgag gccgccacct cggatccgga gtcgatgcag
     2581 cagcgcttgt acttcttgcc cgagccgcag gggcatggat cgtttcgacc agcacggctc
     2641 gtgctggacg gcgtggccgc tgacgtatgg gacagcgcgt tcctccgcac ctcctggaac
     2701 gtcttgtagg tggcgccgag cagcccatcc atgcttcgac atagaatctg ctctggctca
     2761 ggggcgaagt ttcgttggat gtcttccgcg gtcttctctg agacgagatc gagccgtccc
     2821 ccgaggtacg ccatcggcgc ggcgaacgac cagtgatcaa cgttgccgag ccacacgggg
     2881 tcaatctcgg ctcctgccgc gtagccctcg gcgaaggcct tgcactcgtc cacgtcctcg
     2941 ggtctcggca tgaccgcctg ctgatgctcg agcgcgtcga ggacgtcgcc gtactgccga
     3001 agcagcagcc cgaagaactc ttcggcctca gctgccccga gtccgccgag gccagcgggc
     3061 agcaccacgg gtaaccaagc cgacggtggc agtaggctcg gggcgaccgc gaccgcgttc
     3121 agcacgccaa gcagcccctc tatgtcgaac ggggagtgag cttcgaggaa gtcggcgagg
     3181 cgatcggcct cgtcgtccga caggggctct acaggcggcg gagagtgggg tggcacgggg
     3241 cgggagcctg cctcgggggg cgccctggcc gcaattccga tcgtggaggg gagcaatatg
     3301 gctggcccga tccgttgccc gatctgtcga tagtgccctt ggcagctgag gctgtcaggg
     3361 cgtgacattt cgacaacgaa acgcagcgcg agaaggaagg gccgatggcg caccccgggg
     3421 tcaagggttc ggacccgtcc agaggatttc cggcaagctg atgtgttcgc gcccccacag
     3481 ctcgagcgcc gccaggttcg tccgaagccg gagttccccg ggctcgagcg gctcgtcggc
     3541 aaggattggg aaatggttga ccaaatcgac ggtgcgagag aagcgcggca tcgggccgct
     3601 cagcaccggc atgccctggg ccgatttacc tgcgggtgcg caacgtttca gagctcgact
     3661 tcgattccgt cgaggtcaac gagatcgcct tcggtgcgtt gcagcgtggt gctcaaagcg
     3721 ggtatcgact cgccaccggc tgcgtctatt cctatggggc aatgaaggtc acgagtggca
     3781 agcagcgctt cgccacctgg cccatcgact tcgtcgacga gcgaccgctg caggccggat
     3841 actacacgca tgctctgacg gtgcagccgg agcgacaacc gggcgacgcg ggcggaatcg
     3901 atagcacgct ttcgaaggac gcggcgccca ggtaggtgtc tgcagcaaca tgcgggctct
     3961 gacgcgatcc gctgcggcac agagccgtgc tcaccgccgg gccgggcccc agcacgtgag
     4021 gacgcagggc cgcttctcgg cacatactct ggacattata tacagtaggc gttatgtttt
     4081 cgagggtggt cgaggttgtc gcggttatcc gcgctcgagc gagatcaaag gggcatcgtt
     4141 tgcactgcga gagctgcgca tccagtccaa cggacgccca ttgcgagtct tctatgcctt
     4201 cgaccccaga cggcaagccg tcttgctcat cggcggggac aagacgggag acgctgggtt
     4261 ctacgaccga atgatccctg tcgccgaccg catctggaag gagtatctcc gtgaaacttc
     4321 ataagtggaa cgacatcaag cgttcgcagc gcgagccggc gcgtgcggaa cgcgttcgga
     4381 agcgagtgga acaggagctg atcgaaatga gcctggcgga cctccgtcgg gaactcggga
     4441 tcacccaagc cgagatggcg aacgccgcag acatggcaca accgcaggtc tcggcgctgg
     4501 agagccgaga agaccatctc gtgtcgaccc tacggcgctg cgtgcgcgcg ctcggtggcg
     4561 agctcgaggt gatcgctgtc gtaggcgaca agcgtgtcaa gcttgccgtt tgacaccgag
     4621 cacgagacgt gggtctgcac cctctgcaga ggctacgagt gtgacttccc cgggtgcgac
     4681 ccggtccggg acgccgagtg gctcacgcgc cactggaaca ctcgcccgtc gtgccgcttc
     4741 gatgccgccg ccggtaagct cgtcccggtt gacctctcca aaacgccctg tccgtccgag
     4801 gtcgcagagg ccgcgctagc gcggtggtgt gccccggagt accgggccct cttggggagg
     4861 attgccgccc gcgacggact gtcgtcggag atgggccgga ccttcgctcg ctgggactct
     4921 cccgaatacg aagacctgct catggccgcg tttgcgcgcg cgaagcagcc cggtgatggc
     4981 tgagcgggac tcgcacctcg ctccgctcgg gcgcgagcag gcggcgcggc gaatcgtgtc
     5041 ggcgcaaggt gacgagggac tgaagacctg gaccgagcgt ctcagccgcc tcgagcggtt
     5101 cgcgttcccc gaggtcggcg ccttgcaggt cacgaagatc cgtccttctc acgtgcgcgc
     5161 caccctcgaa gcgatggctc gtcagaggct cagcaggcgg acctgtacgc acctcaaggt
     5221 cgacatctcc agcgtgcttg gcgatctctg gcgagctgaa gtcgtgtcgg agaacgtcgc
     5281 cctgcgggtc acggtgccga agacagcggc tgtcgacgcc cccggagcgg gtgattctga
     5341 gcgacgcgga gttcgaggcg ttcatgtcgt gccccgatgt cgatccggag ctgcacgtcg
     5401 tggcgctcgt gtcccgcacg ttcaggggcg cgcggacctc ggacctgcac gcctgggact
     5461 ggagccacgt ggacaccgcg ggctgggtcg aacgcacacg tgcccaggcc gaagacgaaa
     5521 accaaggctt cagcggcact cgatcactga tccacctcgc ggcacggtag tgatccatgg
     5581 cgccgtccgc caggcacgcg ggcgcggcac gatagtgatc caccccccac tcgcagcggc
     5641 gcgagggcga ggttaggtca ggcgctca
//
LOCUS       DASTKU010000231         4775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1161292, whole genome shotgun sequence.
ACCESSION   DASTKU010000231 DASTKU010000000
VERSION     DASTKU010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161292"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VFQ61_08610"
     CDS             complement(<1..335)
                     /locus_tag="VFQ61_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9954553.1"
                     /translation="MTDTKPFQATTREHLIHLLAEAAELEHNLMCTYLYAAFSLKRQS
                     SEFSDEEAHAVARWRANILRVAVEEMGHLASVWNITSALGAIPQLGRGNFPLDPGMLP
                     AGLVVKLVP"
     gene            1286..2488
                     /locus_tag="VFQ61_08615"
     CDS             1286..2488
                     /locus_tag="VFQ61_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954554.1"
                     /translation="MNKLVSLFAGAGLLAMGCASGSESADELTTLESTIAASPATLTY
                     TSDTGTAYCATITMPNAFSTTGTGWFAGVDVKTNTITSVSGAVKSASTGKVVFKPSGA
                     VAIPGGANASFSFCATASGPTVRPTIVAWNITTAPYATCQTNSGVYPTKAALAVTMAM
                     EVGRWQPNTDLAIQGTIYQYGSRVILSSAALSACAANGKTGCPNTKALLGQQDASFVD
                     QNLFNATNYWSDLENSFGRQTNLIQDLTRNNSGALPPAHKLTLVAGPTNIGGPNACGP
                     HYVFQVDNTAGQPLTSTQAANMVNAMCFYGAGSCGPNPYIGFQVTTANGCPSGKTCIA
                     IDPEDGDTGSTSTTSAGSAPTYPANRVYNPDNSLLGTACITIAGTLKTLVSQCSNKPA
                     TCGYLYCM"
     gene            2693..3439
                     /locus_tag="VFQ61_08620"
     CDS             2693..3439
                     /locus_tag="VFQ61_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954555.1"
                     /translation="MVSERRDSNMAGTRAGPSSRPLAVFRLVLWGFAAVFVGVGGWLF
                     FVASADAAEEARRADTTSRSEATDFPRLTPGTATLLVGELVAREPVGPQGFVAYQKES
                     YLRTATQGASKGRTEWMTLSVPPPLIAVKRGDVFVPICNRDYAMSNRPHRWQSDTIPV
                     WRSVGDSTIRLSGFKAGDALTVDGVVTRSSADGAEPCLRAKAIFAGAPTEYVAELRSG
                     VKTTKIVGAILAGFGLLALALGRVLGRTRG"
     gene            3569..4045
                     /locus_tag="VFQ61_08625"
     CDS             3569..4045
                     /locus_tag="VFQ61_08625"
                     /inference="COORDINATES: protein motif:HMM:NF042760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET9954556.1"
                     /translation="MSHYESRAPYIAQHPSALAVYCSDGRFTQSVEELLLSLGEERLD
                     TLTLPGGPALLDHTSSSFSSLEVVRDSASFLIRGHSIRRVVLIAHAECGYYRARFQYE
                     SAQAMQRRQLNDLAAAARWIEASHRGVAALTFYATARERPNGTRGVVFEQVGIAPR"
     gene            complement(4074..>4775)
                     /locus_tag="VFQ61_08630"
     CDS             complement(4074..>4775)
                     /locus_tag="VFQ61_08630"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET9954557.1"
                     /translation="LTAAGQDVFDTARDLEDIVLGLERRVHGRDARLAGPVRVTLPDP
                     LLAPLLPLFRQFTRAHPDIALTLAIDTGYADLAHREADIAIRAAAEPPSELVGRRIAT
                     IGVGVYGSREYLKGRPPRDLSSLDWIGWEQGSAMTFARWIHENVPGERIALRVNHGWA
                     LRDAIDEGFGVSLLPCAAGDKMRTWQRVRREPALTAPLWVLTHKDLRTTARVRVLRDF
                     LSDSISAERKTYEGK"
BASE COUNT          893 a         1586 c         1440 g          856 t
ORIGIN      
        1 aaaggcacca acttgacgac caatccagct ggcaacattc ccgggtcgag cgggaagttt
       61 ccgcgcccga gttgagggat cgcgccgagt gccgaggtga tgttccatac cgaagcgagg
      121 tgccccatct cctcgaccgc cacccgaagg atgttcgctc tccaacgcgc gacagcatgc
      181 gcctcctcgt ctgagaactc ggaagactgc cgcttgaggc tgaaagcggc atagaggtac
      241 gtgcacatca gattgtgctc gagctccgcg gcctccgcga gcaggtgtat gaggtgctcc
      301 ctcgtcgttg cttggaacgg cttcgtgtcg gtcactcctc tacctcttac gacaccggtc
      361 gggactccag cgctttcttg gtttcgccgc aaagtcgcac aatttcgctg acctcgttcg
      421 ctggctctct gcgtactgat ctgcgctccg caatcacgag aggccctact cgaggcctcg
      481 cgggtgcggc cctgcgcgag ctacatccgc aagctcgccg cgctcactgg tctcggggtt
      541 gcgtcccgcc cggctgcata cgcaggacgc ctcatcttca tgggtcaggc ggaatcaatg
      601 cctgcggctc aacccatcag cgagcccgga cgcggagcgc gccgaagcat cggccagcag
      661 ttcgcgcccg tggaccaggc tgctcgcgcc tccgcaatca agccagacgc tcgcgcgttg
      721 gacattcgga aacggctgtc tttctcgccc cccaacaccc gatcccggcc agtgatccac
      781 agaagtgaat cacacccaat gtatacaaat cagttgcgtg cgcggcgaag gcgatctcca
      841 ctcgccaaac tgcgcccatt gttctatggt cgcgcactcg ccgacacacc ccttggggtg
      901 atcgctaaga ttgcgagctc caattcccca cctctctata cgagcagagt gatgatttcc
      961 acggtcagtt ggtaccagat tgcgacgagc gagagtccca cggagagatc cgcgagcacg
     1021 cgcgttttct caagggggga tgccgttgca cagcgttgcg caactcggct gcgcgagttg
     1081 tgcacctacc ccggcgctat gcacaactcg tacgggctgg caccgaaggc gcgctgacgt
     1141 gcgctggatt cctacaatca tgaatttctc gacacaaatt cgaatccatc ttcggttcaa
     1201 cccccgtggc gaacatcgcc tggcgcggca cgccgcctgc aaagcctagc gagcgaaggt
     1261 tcgagaaagg aaacctaaat acatcatgaa caaactcgtt tcattgtttg ctggtgccgg
     1321 tctgcttgcc atgggttgtg cgagcggctc cgagagcgct gatgagctca ccacgctcga
     1381 aagcaccatt gcagcatccc cggcgacact cacctacacg agtgacaccg gaacggcata
     1441 ctgtgcaacc atcactatgc cgaacgcctt cagcaccacc ggtaccggct ggttcgccgg
     1501 cgtggatgtg aagaccaaca ccatcacgag cgtgagcggt gcggtgaaga gcgcctcgac
     1561 cggcaaggtc gtcttcaagc cgagcggcgc ggtcgctatc cctggcggcg ccaacgcttc
     1621 attcagcttc tgcgccaccg cctctggccc caccgtgcgc cccacgatcg tcgcgtggaa
     1681 catcacgacg gcgccctatg caacctgcca gactaactcc ggcgtttacc cgaccaaggc
     1741 cgctctcgcg gtcacgatgg ccatggaagt cggccgctgg cagccgaaca cggatctcgc
     1801 gatccagggc acgatctatc agtatggttc tcgcgtgata ttgagctcgg cggcgctctc
     1861 ggcgtgtgcc gcgaacggca agacgggctg cccgaacacc aaggctctcc tcggccagca
     1921 ggacgcgagc ttcgtcgatc agaacttgtt caacgcgacc aactactggt cggatctcga
     1981 aaactcgttc ggccgccaaa ccaacctcat tcaagacctc acgcgcaaca actccggtgc
     2041 cctgccgccc gcgcacaagc tcacgctcgt cgccggcccc acgaacatcg gcggtccgaa
     2101 cgcctgcggc ccccactacg tgttccaggt cgacaacacc gcgggtcaac cgctcacgag
     2161 cacgcaagcc gccaacatgg tgaacgcgat gtgcttctat ggtgccggct cctgcggccc
     2221 gaacccgtac atcggcttcc aggtgacgac ggcaaacggc tgcccctcgg gcaagacctg
     2281 catcgccatc gacccggagg acggcgacac gggttctact tcgacgacca gcgcgggtag
     2341 cgcgcccacg tacccggcca accgcgtgta caaccccgac aacagcctcc tcggcacggc
     2401 atgcatcacc atcgctggta cgttgaagac gctggtttcg caatgctcca acaagccggc
     2461 cacctgcggc tacctctact gcatgtgatt cacgctgcag ttacgctcct aaacacaggt
     2521 ttctgaccga accttcgaaa gcgaggctgg cactcccggt gccggcctcg cggctttttg
     2581 tgaggcaccg ctcgagccaa gagccttcgc aactccattt cgtgctcagc ggtgccgctc
     2641 ccttgccaag ttcctgggcc tcggcccatg ctggctcgac gaaactcgca agatggtgag
     2701 tgagagacga gacagcaaca tggcaggaac gcgcgctggt ccttcgtcga ggccactggc
     2761 cgtctttcga ctggttctat ggggcttcgc ggctgtcttc gtcggcgtcg gcgggtggct
     2821 gttcttcgtt gcatcggcag atgcagcaga agaggcacgt cgggctgaca cgacttcgcg
     2881 cagcgaggcc accgacttcc ccaggctgac gcccggcacc gcgacgctgc tcgtcgggga
     2941 gctcgtcgcg cgcgagcccg tcggtccgca agggttcgtg gcgtatcaaa aggaaagcta
     3001 cttgcgcacg gcgacccaag gcgcatcgaa gggccgaacg gagtggatga cgctgagcgt
     3061 cccgccgccg ctgatcgcgg tgaagcgagg cgacgtgttt gtgcccatct gcaatcgaga
     3121 ctacgccatg agcaaccgtc cgcaccggtg gcagtcggac acgattccgg tgtggcgaag
     3181 cgtcggagac tcgacgattc gcttgagcgg cttcaaagcg ggtgacgctc tgacggtgga
     3241 cggggtggtc acgcgctcat cagcagacgg cgcggagccc tgtcttcggg cgaaagccat
     3301 ctttgccggc gccccgacag agtacgtggc ggaacttcgg agcggagtga aaacaacaaa
     3361 gatcgtcggt gccatcctcg ccgggttcgg cttgctcgcg ctcgcactcg gtcgcgtgct
     3421 cggccgaact cgtgggtgat tgcggaggct ccaaacaccg atcggcccga ctcggggagc
     3481 tcgaacgagt ccgacggaga gcatggctgg tgtgggcagc ttccggcgac ccattgcgct
     3541 ctcggcagaa gcaaggtttg ctcgaacgat gagccactac gagtcgcgtg cgccgtacat
     3601 cgcgcagcac ccttcggcgc tcgcggtgta ctgctccgat ggccgcttca cgcagtcggt
     3661 ggaagagctt ctgctctcgc tcggagaaga gcggctcgac actctgacat tgccgggcgg
     3721 ccccgccctc ctcgaccaca cttcgtcgag cttctcttcg ctcgaagtgg tacgagactc
     3781 ggcgtctttc ctcatccgcg gacattcgat tcggcgcgta gtgctcatcg ctcacgccga
     3841 gtgcggctat taccgggccc gattccagta tgagtccgcc caagcgatgc agcggcgtca
     3901 gctcaatgac ttggcggcag ccgctcgctg gatcgaagcg agccaccgcg gagtggctgc
     3961 gctcacgttc tacgccactg cacgcgagcg ccccaacggc acgcggggcg tagtgttcga
     4021 acaggttggg atcgcgcccc gctgagccgg ctcgcagcgc cttccgccgc cgctcatttg
     4081 ccttcgtaag tctttcgctc agcggaaatt gagtccgaca agaagtctct caaaacgcgc
     4141 acgcgcgcgg tggtccggag gtccttgtgg gtgagaaccc agagaggtgc ggtcagcgct
     4201 ggttcgcggc ggactcgctg ccaggtcctc atcttgtccc ccgctgcgca cggcagcagc
     4261 gagactccaa agccctcgtc gatcgcgtcg cgcagagccc aaccgtggtt cactcgcaac
     4321 gcgatgcgct cacctggcac gttctcgtga atccagcgcg cgaacgtcat cgcagatccc
     4381 tgctcccagc cgatccagtc gagcgaagag aggtcgcgcg gcggccggcc cttcaggtac
     4441 tcgcgtgaac catacacacc gactccaatc gtggcgatgc ggcgcccgac cagctcggag
     4501 ggtggttcgg cagcggcacg aatggcaata tccgcttcgc gatgagcgag gtcggcgtac
     4561 ccggtatcga tggcgagggt gagcgcgata tcggggtggg ctcgtgtgaa ctggcggaac
     4621 agcgggagca aaggagccaa taatggatcc ggcaaggtca cgcgaaccgg accggccaag
     4681 cgagcgtcgc gaccatggac ccgtcgctcg agtccgagga cgatgtcttc gaggtcgcgc
     4741 gccgtatcga atacgtcctg tcctgccgcc gtgag
//
LOCUS       DASTKU010000232         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_937809, whole genome shotgun sequence.
ACCESSION   DASTKU010000232 DASTKU010000000
VERSION     DASTKU010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937809"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..391)
                     /locus_tag="VFQ61_08635"
     CDS             complement(38..391)
                     /locus_tag="VFQ61_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954558.1"
                     /translation="MALLAFAFGACSADESREPEPYHNTVGSFLARESEAGRFELLHV
                     LASLSPEAGGRYLLMLQYASTLQSVELARQLAQNLPTNSVPVIAGMREADLIAKPVTV
                     VWFRTPTEAELDVLR"
     gene            complement(460..1716)
                     /locus_tag="VFQ61_08640"
     CDS             complement(460..1716)
                     /locus_tag="VFQ61_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954559.1"
                     /translation="MSRTKLPKLSRLSLVLAHAVWSSALHAQSSPGVERISLGPTALK
                     LSAFEQRAERGILTPVPIEITLPAQLKAARVLVHYRIHGSDAWSALELRRSGPRWWGA
                     IPCLEVSTITGDIRFYLRVHDARGGVIAFSGSRHQPYRIAIMHQPQEQASGAGRCPDP
                     SDCPPGLPGCPSPKVDKIPCSSDRDCEGGLSCGWEGYCELDDRAHNWLSAAAEYGVGV
                     VALAGACSVESQESSGYLCTREGDGVTYTGNPVYTNEPLAVGAVPLRFSVGYERLLDY
                     RHSVAVRFGYAVLGSGPTARGGAAFVPYAAEMRGTHFFSADPFARAGLHPYVFLGAGY
                     AMYDIRVRTRVREDPFAASSQGGNDLEQNLEVWKRAGDAFIDLGLGGLLLTSKRVGIL
                     GELKAAQVFPFGALVMTAQLGVQAGF"
     gene            complement(1713..3272)
                     /locus_tag="VFQ61_08645"
     CDS             complement(1713..3272)
                     /locus_tag="VFQ61_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9954560.1"
                     /translation="MSIPLAPAGDAALLVQSPEARGASRLRARLLAEYASEPLVLISP
                     SQQSYQVVRDQLLVHAQLSFSLRHRFLFDLDVPYAARVQGETPPTDLPSQSLYESVPL
                     PAETRAWGAISVGARARLLGGAAGPAWGLGARVLLPGSSESYTGDSRPELHVFTSTGF
                     QDKRAAWAVELGYALRRGRTLPGVLPTRVGSSAHAGVMARFAIDPKARIWIGPELRGS
                     FVTAQGASWFDPRSTTVQLLLGARVRPFGDALELGGGFGPGLGQGVGSADYRAFLFVG
                     WSTEEPPPPPDRDEDGVADAQDVCLNLFGQPSRDPLMNGCPALPLDTDSDSIPDVFDA
                     CPREAGVASAQRSTHGCPASLDPDQDGILSARDACPERAGIASADPAQNGCPATQAPP
                     RAQLLDRSIVISEQVQFETGTARLRAESAAILTEVLEILKAHSEIQQVEVQGHTDDTG
                     NAELNLRLSEARARSVAGWLAEHGIATSRLSPKGYGQTRPLGDNASEAGRARNRRVEF
                     RILTRGEVHEP"
BASE COUNT          686 a         1140 c         1047 g          574 t
ORIGIN      
        1 aagaacgact agtccggaag tcgagcgctt cgatgctcta gcgaaggaca tcaagttctg
       61 cctcggtagg ggtcctaaac caaaccaccg tcaccggttt cgcgatcaag tcagcttctc
      121 tcatgcccgc gatcaccggc acgctattcg tgggtagatt ctgcgccagc tgcctggcca
      181 attcgacgct ctgcagcgtg ctcgcatact gcagcatcag gaggtacctg ccgcccgcct
      241 ccgggctcaa cgacgctagc acgtgcaaca gctcgaagcg ccccgcctcg ctctcgcgtg
      301 caaggaaaga accgaccgtg ttgtgataag gctcgggttc ccggctttcg tccgccgagc
      361 aagccccgaa agcaaaggcc agcagtgcca tgagcctgat tgctccaacc cgagcccagc
      421 aagatcggga attcggccgc gaccaatatc tgtttgccat tagaaccctg cttgcactcc
      481 gagctgcgcg gtcatgacca acgctccaaa aggaaacacc tgcgcggctt tcagttcacc
      541 gagtatgccg acgcgcttgc tcgtcagcag caacccgccg agtcccaagt cgatgaatgc
      601 atcgccggcc cgtttccata cctcgagatt ctgctcgagg tcattgccac cctggctgga
      661 cgcggcgaag ggatcttcgc gtactctcgt cctgacccgg atatcgtaca ttgcgtagcc
      721 ggcgcccaga aacacgtacg ggtgcaagcc cgctcgcgcg aagggatcgg ccgagaagaa
      781 gtgagtgcct cgcatctccg cggcgtaagg aacgaacgcg gctccgccac gcgcggtggg
      841 gcccgatccg agcacggcat aaccgaatcg cacggcgacg ctgtgccgat aatcgagcag
      901 acgttcgtaa ccgacactaa aacgcaacgg cacggcaccg accgccagag gttcattggt
      961 gtacaccgga ttgccggtgt acgtgacacc gtcgccctcg cgtgtgcaca ggtagccgct
     1021 actctcttgg ctctccacgc tgcaagcgcc ggccagcgcg acgaccccta ccccatactc
     1081 cgcagctgcg gagagccagt tgtgagcgcg gtcatccaat tcgcaataac cttcccagcc
     1141 gcacgacagg ccaccttcgc agtcgcggtc ggaagaacaa gggattttgt cgactttggg
     1201 gcttgggcaa cccggcaacc ccggtgggca atcagagggg tctgggcagc gccccgcgcc
     1261 tgaagcttgc tcctgcggct gatgcatgat cgcgattcgg taaggctggt gacgcgaacc
     1321 gctgaaggcg atcactccac cgcgcgcatc gtgcacacgc aagtagaatc ggatatctcc
     1381 ggtgatggtg cttacttcca aacacggaat cgccccccac caacgcggcc cgcttcggcg
     1441 gagttccagc gcgctccacg catcagaacc gtgaatgcga tagtgcacga gcacgcgggc
     1501 cgccttcagc tgcgcaggca gcgtaatctc gataggcacc ggagtgagaa tgccgcgctc
     1561 cgcgcgttgc tcgaaggccg agagcttgag cgccgtcggg cccagagaaa ttcgctccac
     1621 tccgggagaa gactgcgcgt gcagcgccga cgaccacacc gcgtgcgcca gcaccaacga
     1681 caggcgagag agcttcggaa gtttcgtgcg actcacggct cgtgcacctc tccgcgcgtc
     1741 agtattcgga actcgacacg tcggttgcgg gcgcgccctg cctcactggc gttgtccccc
     1801 aagggacgag tctgaccgta acccttgggt gagagccggc tggtcgcgat gccatgctcc
     1861 gccaaccaac ccgccaccga ccgggcccgc gcctcggaga ggcggagatt gagctcggcg
     1921 ttgcccgtat cgtccgtatg cccttgcacc tccacttgct ggatttcgga gtgcgctttc
     1981 aggatctcga gcacctccgt caagatcgcc gcgctctccg ctcgaagccg cgcggtaccg
     2041 gtttcgaatt ggacctgttc agagatcacg atgctccgat cgaggagttg ggcccggggc
     2101 ggcgcctggg tcgccgggca gccgttttgg gccggatcgg ccgacgcaat gcccgcgcgc
     2161 tccgggcagg cgtcgcgtgc gctcaggata ccgtcctggt ccggatctag agacgctggg
     2221 cagccgtgcg tgctccgctg agcgctggcg acccccgcct ctcgcggaca agcgtcgaac
     2281 acatccggaa tcgaatcgct gtccgtgtcg agcggcagag cggggcaacc attcatgagc
     2341 ggatctcgtg agggctggcc gaacaagttc aagcaaacat cttgcgcgtc ggctacgcca
     2401 tcttcgtctc gatcgggcgg aggcggaggc tcctcggtag accagcccac gaataggaag
     2461 gcgcgatagt cagcgctacc aacgccctgg cctaggcccg gtccgaaacc accacccaac
     2521 tccagcgcgt caccgaacgg acgcacccga gctccaagca gcagctgcac ggtggtgctg
     2581 cgcggatcga accaggacgc gccctgcgcc gtgacgaacg agccacgcag ctctggccca
     2641 atccaaattc gcgctttcgg atcgattgcg aaacgagcca tgacaccggc gtgagcgctc
     2701 gatcccacgc gcgtcggcaa cacgccgggt agcgtgcgcc cccttcgcag cgcgtatcct
     2761 aactcgactg cccaagcggc tcgcttgtct tgaaagccgg tgctggtgaa aacatgtagt
     2821 tctggcctac tgtctccggt gtacgattcg gacgagcccg gcaacagcac ccgagcgccg
     2881 agaccccaag ccgggccggc cgcgccaccc aacaggcggg ctcgggcccc aaccgaaatt
     2941 gccccccaag ctcgcgtctc agcgggcagg gggactgatt cataaaggct ttgactcggt
     3001 agatccgtgg gcggggtctc gccctgcacg cgagccgcgt aaggaacgtc gagatcgaac
     3061 aagaagcggt gtcgaagcga aaacgacagc tgcgcatgga ccaacaattg gtcgcgcacc
     3121 acctgatacg attgttggga agggctgatc aacaccaacg gctcgctcgc gtattccgcg
     3181 agtagtcgtg cccgaagacg cgaggcaccg cgtgcttctg gagattggac cagcagcgcg
     3241 gcgtccccag cgggcgcgag cgggatggac aagttcagtc cgggctccgc accgcgcgca
     3301 acacgaggaa gcactagtgc accgcatgtg acttgagttc aatttttgga ggggttgcct
     3361 cgtcgaacga tgcgtggaac gaagcaacga cttgaagcta agcgccgcgg acagaaagac
     3421 cctgcagaag ttaatcgcgc gtccgtc
//
LOCUS       DASTKU010000233        11200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1831182, whole genome shotgun sequence.
ACCESSION   DASTKU010000233 DASTKU010000000
VERSION     DASTKU010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11200
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831182"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFQ61_08650"
     CDS             <1..448
                     /locus_tag="VFQ61_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954561.1"
                     /translation="VLARTPPIDLWPVLAALITQFAPSFSPQAVDARKLGEAKARIAA
                     AAPTEVDNELVSLALEVSASIGNRASQLSTALYQWGNRTALLSVGDPSAALRAVALAT
                     TGNQGPPPNGPDRIKWIVRNAEARDLAIFSVSEGYSEARRRLGLRA"
     gene            625..2397
                     /locus_tag="VFQ61_08655"
     CDS             625..2397
                     /locus_tag="VFQ61_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954562.1"
                     /translation="MRYARQGSQRNHRAPAVLMSQARVPIIQRLEHLERQLAFFEADR
                     EARMLCWALAPDEQRLMEGCLQKRIEENSEREFLVLLDEPFGSEIGHGLSLVRELKAL
                     TDAETEAFAQSETYDIPWSPPPLPRELRPRKGRAPDPLYFRDALLSLHEHLCAMDGLL
                     LVALWPEAISDSEQYAQWLAALVPLLEVPHVRVLLPCERGGLWAQTLARTHAPRVRWI
                     EANLNMPAALEEICAAAEDHTEPSGQYRAGFVRAMNALAAGNYATAREHAETSAALAR
                     HEAWPHLEFAAHQLLGSGALGLGQPREAVARFEAAERAVAHDLDHGKNTLTPLLLQAR
                     LCKGSAWMVSGNPTQAAQIFEREALPVAHVLGDRAMQLEALRLAAQCLERGGDTGGAW
                     KRAVAAVAVGASIPPQARSETSLAQVGELLLQLLKGFGHGKQRAAIRAKFNDLLGPDW
                     EGKSVPQSATAEDMLQSPLAEKAPPLLDDSDRDPVEQTFDISASIAAEYLGKLVKGRS
                     GASAASVEAQGPSDQTWDASALVSDPADRTWDYPGGIPQPDAPLPGPGVALESAGAVA
                     AARVPAPEPSRGPPSRPPRSRGDS"
     gene            complement(2410..4743)
                     /gene="pbpC"
                     /locus_tag="VFQ61_08660"
     CDS             complement(2410..4743)
                     /gene="pbpC"
                     /locus_tag="VFQ61_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HET9954563.1"
                     /translation="MSGWRAILERLLLVLSGTLLVGHLSLELWASAEGMPEALREPLR
                     ASSLALYDRHGRLLRELSWKAGRVSAEVSLDQVSPDLVRALIATEDRRFYEHDGVDVL
                     AMARALVDAARAGRIVSGASTLTQQLVKTSTRRPRTLWGKLREVVVARRIEREFDKPR
                     ILTEYLNRVEFGPNLRGVEAASESYFGKPARDLDLAESATLVAIVKGPSWYDPRRHSE
                     RTTARRDHVLRQMQLKGAAAPERVRAALQEPMRVQPALAGRAPLHWAFALASGELSPG
                     LEPSRVRRLHTTLDAALQQEVETTVRSLRSRLGEIGASAASVLVIENRSGDVLAYVGS
                     PDYLDAAALGSNDGTRALRQPGSALKPFVYAAAIERLGYDAATVLPDLALELETPNGR
                     YRPQNYDGREHGPVRLRVALGSSLNLPALTVARRVGPSHVLELLRRFGFRSLTRDASH
                     YGAGIALGDGEVRLSELAQAYAALARGGTLARLRYVRFAELSGGARVWFDASTQERVI
                     DERTARLVSDILADDAARSPAFGRASALDFDFAAAAKTGTSKGFRDNWAVASSSEITV
                     AVWVGNFDGRPLVRSSGVSGAGPLLNSILNSAMRSRSQGARRAIAEDSAFEPVRICAL
                     SGLRAGAACSAQLTEYFLRERAPQRTCDWHQSRAGEIFEAYPELYRNWAARAGRPLLS
                     SSLDAAARPGSTAEPRIVWPRSGARFVLNPSGKSEIVIVGAGLDPSRPAELRADGKRV
                     HSAEFPFEFVWPLRLGDHELSIEQGTSSLPVRVSVAR"
     gene            complement(4740..10304)
                     /locus_tag="VFQ61_08665"
     CDS             complement(4740..10304)
                     /locus_tag="VFQ61_08665"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HET9954564.1"
                     /translation="MLLCTCSSANKPTTSTGRALQPVGEQRGAQEGAFRVVFAAPRGE
                     AAAGAELSLVFSQALRGLEEAKDAPELPLSPRVPGKFRYVGTRALVFVPDEGQLPGGT
                     AFRLEVPKGFRSVTGGTLAEPFVLQFETPRPRLTGSEPRTGAEGVLPGASLELTFNQA
                     VTPEQVQRFGRLVVNGRALGFASSRPRDKELNVVSVKPREVLPRDRAVEFVLDAGLLG
                     AEGPLTAGIEQKVSFQTLAPLHVTGVECSREKEGAPCFPGSSLGVMFSSPVLRTAAEK
                     LVRVESSELHFEDHEATETTTYLDLPVRLAPGRTYTVRLGKGLVDIHGQRMDRDFAQT
                     LKVADLAPAVAVGLAGAREGTLVPATPGLLPVGAVNAREAALVYAALSPEDVLRFDRD
                     QVAPQDFLQSKAGRVQVTLPLGVRNRMSRADVDLSKVLGAQRGPVALESSFLNKEGAR
                     EKAVQLMRVSDLALSAKFSRHGSELWVTHLSSGKPEPGAKVALWLANGKRFEYAADAA
                     GRAAIPARDFAPKFDYESPDRKALLFVTSQSGTDWTYERLSDVMDSWSLPVPVDDSGE
                     LAVRGLLFSDRGVYRPGEVVQVKGIVRRDTARGSAVPVNEKVTLALNNPSGEEVSRTE
                     LTVSRYGTFNAAVNVPKLAELGDWVFTAALGQQKLRHAVGVLEYRPQEFKVDVSAPAT
                     AIRGQRVDVRVIGEYLYGAKMAGAQANYQATRSEADFTPEGRESFVTNALDYYADSAE
                     AGYDLGVLSSQAGALDATGVFVSPLELSLPSQRGAERVVIEGEVQDVSRQSGAHSTSI
                     LVHPADFYLGLERPPQSFVDAPSVLEPGVLALEPSGKAVAGRKVRLELVQRRYTYAHE
                     DRGGAYPALVSKRVERVLSSCELTTAATKTSCKLPLNEPGYVVVRARSQDNRGNPVEA
                     ALGYYAFGAGEASFASRDAGDVELVLDRKTYRVGQKARVLVKSPFPEAEAWITIERAD
                     LQRSERKVLRGSMPWFELDVTEDMRPNAFVSVALWRPIDAARSRTPGAAYRMGYAELR
                     VEPESRRLAVHVKPSVSAAKPGDEVGVDLQVLDHQGKPADAELALYVVDEGVLMLSGY
                     KTPDPAPVFSAPRPLQVMTLETRQRLAYVDPNELNAVLGIGKGAPGGGGGEGAGRSDF
                     RSTALYVPSLVTDAQGRAKTRFKLPDNLTRYRVMAVAAGKADAFGFGAANLTVSRKLM
                     ARPALPRFLRAGDVFDASVVVSAKAFDPGDVTTKLSVDGLTPLGASVAQVRPGRDGQR
                     EVRFRLRADRAGEARLTFDVAGNGEKDRVTSTRAVQIPSRIESVALSGSTAGVAEEKL
                     GALGGLRSDVGALDLSLASSALVGVDAALTSLADYPYSCTEQLASRLLPAGPLASLSR
                     DYGFSGLEALNRSVPTLMRDILARQRGDGGFGWWPGDERSEPWVTAYALWALHGTQRS
                     DAKVPAQAFEDGKRYLREWLTQMGEAPERLAIGALALDVLARLGAPDAGYLRQVYGLR
                     ARLPLFARALLLDAVSIARLPGPEQAELVRDLEQHVRVLGATANVLDGEQPEVYAALL
                     DSSTRTQAMVLLALVGARPHHPLLVPLARGLLDKRVGGTFRTTQEAGFGLLALDAYRK
                     SEEAATPEFEAEVRLGDARLFQAEFKGRGLRAQQRRIEMATLMRAAGQPLRFERDGSG
                     TLFYQARLSYARAALPEAPLDAGFYVERRRRPVTPTELSQLARALPPETRTSFAGADW
                     VLTELTVTSSEAREFVVIDDPIPAGFETVDSALVTSSGRYAALESTEEDSPFDAEGRW
                     REGYSSRPRRELRDDRAVFFVDHMAPGIHRFRHLARATTLGQFVVPPPHVEAMYAPEH
                     FGRGAKEVVEVK"
     gene            complement(10685..>11200)
                     /locus_tag="VFQ61_08670"
     CDS             complement(10685..>11200)
                     /locus_tag="VFQ61_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954565.1"
                     /translation="LGSALQYYELINTQWPTDPSAPPADIQDYPSNITNKSGGKPTPV
                     FLINAVMETYFQGGASSANGAGKPEQVHLSVNEDGSAGSPGYDVPSTNGGNTPLDFAE
                     GFCRSVGANCGDAAGKLVLSTESCMGCHSSAGIAKAAPSTAGGTYQFASQLSADFSWL
                     LQQKAQPRAAK"
BASE COUNT         2022 a         4092 c         3309 g         1777 t
ORIGIN      
        1 cgtgctcgcc cgcacgccac cgatcgactt gtggcccgtg ctcgccgccc tcatcaccca
       61 gttcgcgccg tccttttccc cgcaagccgt cgacgcgcgc aagttgggcg aggccaaggc
      121 ccgcattgcc gccgccgccc ccaccgaagt cgacaacgag ctcgtcagcc tggccttgga
      181 ggtcagcgcc tcgatcggta accgcgccag ccagctctcg acggcgcttt accagtgggg
      241 aaatcgcacg gccctgctgt ccgtcggcga tcccagcgcg gcgctgcgag ccgtagcgct
      301 cgcgaccacc gggaaccaag gaccaccacc caatggcccc gatcgcatca agtggatcgt
      361 gcgcaacgcc gaggcgcgcg acctcgccat cttcagcgtc agcgagggct attcggaagc
      421 ccgacggcgg ctcgggctac gcgcgtgaag gcaggcgcgg actgacccga actccgctcg
      481 ttcggagttc gggcgcggcc cgttggagct ggatggcggc cccatcgtgg acgggcgcga
      541 atacccccgc aatggccccg tagcgcagcc tcactcctcc ccgtttcgtc cttccaccgc
      601 gctcgccgcc gtgggctggc gaagatgcgc tacgcacgtc aaggttctca gcgcaatcac
      661 cgagcccccg cggtactcat gagtcaggcg cgcgtcccga tcattcaaag gctggagcac
      721 ctcgagcggc agctcgcatt cttcgaggcg gatcgagaag cgcgcatgct gtgttgggcg
      781 ctagctcccg acgagcaacg cctgatggaa ggctgcctgc agaagcgcat cgaggaaaac
      841 tccgagcgcg agttcctcgt tttgctcgac gagcccttcg gctccgaaat cggtcatggc
      901 ttgagcctcg tgcgcgagtt gaaagcgctc acggacgcag agaccgaggc gttcgcccag
      961 tccgaaacct acgacattcc ttggtcgccg ccgcctttac cgcgagagct caggccacgc
     1021 aaaggccgag caccggatcc gctctatttc cgagacgcac tgctgagtct gcacgagcat
     1081 ctctgcgcga tggatggcct tttgctcgtg gctctgtggc ccgaagccat tagcgattcg
     1141 gagcagtatg cgcagtggct ggcggctttg gtgccgctgc tcgaggtccc acacgtccgc
     1201 gttcttctcc catgtgagcg tggcggactt tgggcacaga cgctggcgcg cacccacgcg
     1261 ccgcgggtgc gctggatcga ggccaacttg aacatgcccg cggcgctcga agagatttgc
     1321 gcggcggccg aagaccacac ggagccctcc ggacaatatc gggctggctt cgtgcgcgcc
     1381 atgaacgcac tcgcggcggg caactacgct acagcccggg agcacgccga aaccagcgcg
     1441 gcgctggccc ggcacgaagc ttggccgcac ctcgagttcg cggcgcatca gctgctcgga
     1501 agtggcgcgc tcggcttggg tcagccgcgc gaagcggtcg cacgcttcga ggcagcggag
     1561 cgcgccgtcg cccatgacct cgaccacgga aagaacaccc tcaccccgct gttactccag
     1621 gcgcggctct gcaaaggcag cgcttggatg gtctccggca atcccacgca agcggcgcag
     1681 atcttcgagc gcgaggccct ccccgtcgcc cacgtactcg gcgatcgcgc catgcaactc
     1741 gaagcattac ggctcgcggc tcaatgccta gagcgtggcg gagacacggg gggagcctgg
     1801 aaacgtgcgg tggccgctgt agccgtgggc gcaagcatcc cgccccaagc tcgctcagag
     1861 accagcctgg cccaagtggg tgagttacta ctgcagctct tgaagggctt cgggcacggc
     1921 aaacaacgcg cggcgatccg ggctaagttc aacgatctgc tgggccctga ctgggaggga
     1981 aagagcgtgc cccagagcgc caccgccgaa gacatgttgc agtcgccgct ggcggagaag
     2041 gccccgccgc tactcgacga cagcgatcgc gatccggtcg agcaaacgtt cgacatctcc
     2101 gcctcgatcg ctgcggagta cttgggtaag ctcgtcaaag gcagatccgg tgcctccgcg
     2161 gcgtccgtcg aagcgcaagg tccatccgac cagacctggg atgcttcggc actcgtgagc
     2221 gatcccgccg atcgcacctg ggactatccg ggagggatcc cgcaacccga cgctccactt
     2281 ccaggacccg gcgtggctct cgagtcggca ggcgccgtcg cagcagctcg cgtgcccgcc
     2341 cccgaaccat cgcgtgggcc cccgtctcgc cctccccgct cgcgaggcga ttcatgatgc
     2401 ccgcggcaat caacgagcca cgctcacgcg cacgggcaag ctcgaagtcc cttgctcgat
     2461 cgatagttca tggtccccca aacgtagcgg ccacacgaac tcgaaaggga actcagccga
     2521 gtgaacgcgc ttaccgtccg cccgaagttc cgccggacgg ctcgggtcca aaccggcccc
     2581 aacgatcacg atctcggact ttccagacgg gttcagcacg aagcgcgccc ctgaacgtgg
     2641 ccaaacgatg cgcggctcag ccgtgctccc aggacgcgcg gcggcgtcca acgaggagct
     2701 gagcaacggt cggcccgctc gagcagccca gtttcggtag agctccggat aggcttcgaa
     2761 gatctcccca gctcgtgact gatgccaatc gcaggttcgc tggggcgctc gctcgcgcag
     2821 gaaatactcg gtgagctggg ccgaacacgc cgctccggcg cgcagcccgc tcaacgcaca
     2881 gattcgaacg ggctcgaagg cggagtcctc cgcgatcgcc cgacgggcgc cctgtgaccg
     2941 tgaccgcatg gcgctgttca agatcgagtt cagcagcgga cccgcgccgc tgacgccact
     3001 cgaccgcacc aaaggtcggc cgtcgaagtt gcccacccag accgcgacag tgatctcaga
     3061 gctcgacgcg acggcccagt tgtcacggaa tcccttggag gtgcccgtct tggcggccgc
     3121 agcgaagtcg aagtccagtg cgctcgcgcg gccgaacgcc gggctacgtg ccgcgtcgtc
     3181 agccaagata tcgctcacca gccgcgccgt gcgctcgtcg atgacccgtt cttgcgtaga
     3241 ggcatcgaac caaactcgcg cgccgccgct cagctctgcg aaccgcacat agcgtaagcg
     3301 cgccaaagtc ccgccgcgcg cgagcgccgc ataggcttgc gcgagctccg ataggcgcac
     3361 ttcaccgtcc ccgagcgcaa tacccgcacc gtagtggctc gcgtcccgcg tcaaagaccg
     3421 aaagccgaac cgccgtagca actcgagcac gtgtgacggc cccacccggc gagccaccgt
     3481 caacgccggc agattgagcg agctgcccaa tgcgacccgc aagcgcaccg ggccatgctc
     3541 tcgcccgtcg taattttgcg ggcgatagcg cccgttcggc gtctcgagct cgagcgcgag
     3601 atccggtagc acggttgccg cgtcatagcc gagccgttcg atcgcagccg cgtacacgaa
     3661 aggcttgagc gcagagcccg gctgtcgcaa agcgcgcgtc ccgtcattgc tgcctagagc
     3721 cgccgcgtcc agatagtcgg gagagcccac gtacgccagc acatccccgg agcgattctc
     3781 gatgaccaac accgacgccg ccgaagcacc gatctcgccc agacgcgagc gaagcgagcg
     3841 aacggtggtc tcgacctcct gctgcagagc cgcgtccaga gtggtgtgca ggcgccgtac
     3901 tcggctcggc tcgagccctg gcgagagctc tccagacgcc aacgcaaagg cccaatgcag
     3961 gggggctcgc ccggcgagcg cgggttgcac gcgcataggc tcctgcaagg cggcccgcac
     4021 gcgctccggc gctgcggccc ccttcagctg catctgccgc agcacgtgat cccggcgtgc
     4081 ggtggtgcgc tcggaatgac ggcgcggatc gtaccaactc ggccctttga cgatcgcgac
     4141 gagcgtcgcg gactccgcca aatccagatc ccgcgccggt tttccgaaat aactctcgct
     4201 cgccgcctcg acccctcgca gattgggtcc gaactccacg cggttgagat attcggtgag
     4261 gatccgcggc ttgtcgaact cgcgctcgat gcgccgcgcg accaccactt cacgcaactt
     4321 gccccaaagc gtgcgcgggc gacgcgtgct ggtcttgacc agctgctgcg tcagcgtgct
     4381 cgctccggac acgatacgcc ccgcccgcgc cgcatccacc agcgcccgcg ccatggcgag
     4441 cacatcgacc ccatcgtgct cgtaaaagcg ccggtcttcg gtggcgatca gcgcgcgaac
     4501 gagatccgga ctcacctggt ccaaactcac ttccgcactg acgcgcccgg ccttccatga
     4561 caattcgcgt agcagccggc catggcgatc atacagcgcc aggctgctcg cacgcagagg
     4621 ttcgcgcagc gcctccggca tgccttcggc gctcgcccaa agctccaagc tcaggtgtcc
     4681 aaccagcagc gtcccactca gcacgagtag aagccgctcc aggatggcgc gccagccact
     4741 catttcactt cgacgacttc cttcgctccg cgcccgaagt gctccggggc atacatggct
     4801 tctacgtgtg gcggcggaac cacgaattgc ccgagcgtcg tggcacgcgc caagtgccgg
     4861 aagcgatgta tccccggcgc catgtggtcg acgaaaaaca cggctcgatc atcgcgcagc
     4921 tcacgacgtg ggcggctcga gtaaccctcg cgccagcgcc cctccgcgtc gaacggtgaa
     4981 tcttcctccg tcgactcgag cgcggcgtaa cgacccgagc tcgtgaccag cgcactatcc
     5041 accgtttcga aaccagcggg gatgggatcg tcgatcacca cgaactcgcg cgcctccgag
     5101 ctcgtcaccg tgagctcggt caacacccaa tctgcaccag caaagctcgt tcgagtctcg
     5161 ggaggcagcg cgcgcgccag ctgggagagc tcggtcgggg tcacgggacg tcggcggcgt
     5221 tcgacgtaga agcccgcatc caggggcgct tcgggcaaag ccgcgcgcgc gtaactcagg
     5281 cgggcctggt aaaacaacgt acccgagccg tcgcgctcga aacgcaaggg ttgcccggct
     5341 gcgcgcatca acgtcgccat ttctatcctt cgctgttgcg cgcgcagccc tcgccctttg
     5401 aactccgcct ggaacaagcg cgcgtccccc agacgcacct cggcttcgaa ctcgggcgtc
     5461 gcggcctcct cgctcttgcg atacgcatcc aaagccagca gcccaaagcc ggcttcttgc
     5521 gtcgtgcgga atgtcccgcc gacgcgcttg tcgagcagcc cccgcgcgag cgggacgagc
     5581 agcgggtgat gaggtcgcgc cccaaccagc gccagcaaca ccatggcttg agtccgcgtg
     5641 gaagagtcga gcagcgccgc gtagacttcg ggctgctccc catccagcac attggccgtc
     5701 gctcccagca cacgcacgtg ctgctcgaga tcgcgcacca gctccgcctg ctcgggcccc
     5761 ggcaaacgcg cgatgctgac cgcatccaac aataacgctc gcgcaaatag cggcaatctc
     5821 gcgcgcagtc cataaacctg ccgcaagtac cctgcgtccg gcgcgccaag ccgagcgagc
     5881 acgtcgagcg cgagcgctcc gatcgccaaa cgctccggag cttcacccat ctgggtgagc
     5941 cactcgcgca ggtagcgctt gccgtcctcg aatgcctgtg cgggcacttt ggcatccgaa
     6001 cgctgcgtgc cgtgcagcgc ccacagcgcg taggcggtca cccatggttc gctgcgctcg
     6061 tcccccggcc accagccgaa cccgccgtcg ccccgctggc gcgccaagat atcccgcatc
     6121 aacgtgggca cgctgcggtt caacgcttcc aagccagaaa agccgtagtc ccgagagaga
     6181 gacgcgagcg ggcctgccgg caatagccgg ctcgccaact gctccgtaca agaatacgga
     6241 tagtccgcga gcgaggtgag cgcggcgtcg acacccacca aagcgctcga agccaagctc
     6301 aaatctagag cgccaacgtc actgcgtagg ccgccgaggg cgccgagctt ctcctcggca
     6361 acgcccgcgg tcgagccaga cagagccacg ctctcgatgc gactggggat ctgcaccgcg
     6421 cgcgtcgaag tgaccctgtc tttctcgccg ttgcccgcga cgtcgaacgt cagccgtgct
     6481 tctcccgctc gatcggcgcg cagcctgaaa cgcacttcac gctgaccgtc tcgccccgga
     6541 cgaacctgcg ccaccgacgc gccgagcgga gtcaacccat cgacgctcag cttcgtcgtc
     6601 acgtctcccg gatcgaacgc tttcgcagac accaccaccg atgcatcgaa cacatccccc
     6661 gcccgcagaa agcgtggcaa cgctgggcgc gccatcagtt tacggctgac cgtgagattt
     6721 gccgcgccga acccaaatgc gtccgctttg ccggcggcga cggccatcac gcgataacgc
     6781 gtcaggttgt ccggcagctt gaagcgcgtc ttggcgcggc cctgtgcgtc agtgaccagg
     6841 ctcgggacgt agagcgctgt acttcggaaa tcactgcgcc cagccccctc gccgccgcct
     6901 ccccccgggg cgccctttcc aattccgagc acggcgttga gctcgttcgg atccacatag
     6961 gccaagcgtt gtcgagtctc gagcgtcatg acctgcagcg gacgcggcgc cgaaaacacc
     7021 ggcgcggggt cgggggtctt gtaaccactc agcatcaaaa ctccctcgtc caccacgtag
     7081 agcgcgagct cggcgtccgc gggtttgccc tgatgatcga gcacctgcaa atcgacaccc
     7141 acctcgtcac cgggcttggc cgcgctcacg ctcggcttca cgtgcacggc caaacgacgg
     7201 gactccggct ccacacgaag ctccgcatac cccatgcgat acgctgcgcc cggagtgcgc
     7261 gaccgggccg cgtcgatcgg acgccagagc gccaccgaaa cgaacgcatt gggccgcatg
     7321 tcttcggtca cgtccagctc gaaccaaggc atgctgcccc gcagcacctt gcgctcgctg
     7381 cgctgcagat cggcccgttc gatcgtgatc caagcctccg cctcgggaaa cggcgatttc
     7441 acgagcacgc gcgccttctg cccgacgcgg taagtctttc gatccagcac caactccacg
     7501 tcccccgcgt cgcgtgacgc gaagctcgcc tcaccggccc cgaacgcata gtagccgagc
     7561 gctgcttcca ctgggttccc gcgattgtct tgcgagcgtg ctcgcaccac cacgtagccc
     7621 ggctcgttca aaggcagctt gcacgaagtc ttggtagccg cggtcgtgag ctcacaactc
     7681 gaaagcacac gctcgacccg cttcgaaacc aaagctggat aagcgccgcc gcggtcctcg
     7741 tgcgcgtagg tgtaacgccg ctgcacgagc tcgagccgca ccttgcggcc ggccaccgcc
     7801 ttgcccgacg gctccagcgc cagcaccccg ggctccaaca ccgaaggcgc atccacgaag
     7861 ctctgcggcg gccgctcgag cccgaggtag aaatccgcgg gatgaaccaa gatagatgtg
     7921 ctgtgggcac cactttgacg tgagacgtcc tgcacctcgc cttcaatcac cacgcgctcg
     7981 gctccgcgct gcgacggcaa agagagctcg agggggctca cgaacacccc cgtcgcatcg
     8041 agcgctcccg cttggctgct cagcaccccc aagtcgtagc cagcttctgc gctgtcggcg
     8101 tagtagtcga gcgcgttggt gacgaatgac tcgcgccctt ccggcgtaaa gtccgcctcg
     8161 ctgcgcgtcg cctgatagtt cgcctgcgcg cctgccatct tggcgccgta caggtattca
     8221 ccgatcaccc gcacatcgac gcgctgcccc cgaatcgccg tcgcgggcgc gctcacgtcc
     8281 actttgaatt cttgcgggcg atactcgagc accccgacgg cgtggcggag tttttgttgt
     8341 ccgagcgcgg ccgtaaatac ccaatctccg agttcggcga gcttcggaac gttgacggcc
     8401 gcgttgaacg ttccgtagcg cgagaccgtg agctcggtcc tactcacctc ttcgccgctc
     8461 gggttattca acgccagagt caccttctcg ttcacgggca cggcgctgcc acgcgccgtg
     8521 tcgcgccgca cgatcccctt cacttgcact acttcgccgg gtcggtacac gccgcgatca
     8581 ctgaacaaca gccctctcac cgcgagttca cccgagtcgt ccaccggaac cggcagagac
     8641 cacgagtcca tcacgtcgct cagccgctcg taggtccagt ccgttccgga ctggctcgtc
     8701 acgaacagca gggccttgcg gtctggcgat tcgtagtcga acttcggcgc gaaatctcgg
     8761 gcgggaatcg cggcccgccc cgctgcgtcg gccgcgtact cgaagcgctt gccattcgcc
     8821 agccagagcg cgaccttggc ccccggctca ggctttccac tcgacaaatg tgtgacccaa
     8881 agctccgagc catgtcgaga aaacttggcg ctcagcgcca ggtcactcac gcgcatcaac
     8941 tgcacggcct tctcgcgcgc tccctccttg ttcaagaacg aagactccag cgcgaccggg
     9001 ccacgctgcg ctccgagcac cttgctcaga tcgacatctg cgcgcgacat gcgattcctg
     9061 accccgaggg gcagggtcac ttgcactcgg ccagctttgc tttgcaggaa atcttgcggc
     9121 gcgacctgat cccgatcgaa tcgcagcacg tcctctggcg aaagcgcggc ataaaccagc
     9181 gccgcctcgc gcgcattgac cgcacccacg ggcagcagtc ccggcgttgc cggaactaac
     9241 gtcccctccc tcgcccctgc caaacccacc gccaccgccg gcgccaagtc cgccaccttc
     9301 aaggtctgcg caaaatcgcg atccatgcgc tgcccgtgga tatccaccaa acccttgccc
     9361 aaccgcaccg tatacgttcg tcccggcgct agacgcacgg gcaagtccaa ataggtggtg
     9421 gtctcggtgg cttcgtgatc ctcgaagtgc aactccgaac tctccacgcg caccagcttc
     9481 tcggccgcgg tccgcagcac cgggctcgaa aacatcactc ccaaggaaga acccggaaaa
     9541 cacggcgctc cctccttctc ccgactgcac tccacgcccg tgacgtgcaa gggcgccagc
     9601 gtctggaagc tcaccttctg ctcgatgccg gccgtcaacg gcccctccgc ccccaacaac
     9661 ccggcatcca acacgaactc gaccgcgcga tctctcggca gcacctcacg tggcttgacg
     9721 ctcaccacgt tgagttcctt atcgcgtggc ctcgagctgg cgaaacccag cgcccgacca
     9781 ttcaccacca gccgcccaaa gcgctgcacc tgctccggcg tcaccgcttg attgaacgtc
     9841 agctccaggc tcgcgcctgg caaaacccct tccgctccag tgcgcggctc ggagcccgtc
     9901 aggcgtggcc gcggcgtctc gaattgcagc acgaatggct ccgccagcgt gccccccgta
     9961 accgagcgaa agcccttggg cacctccaag cggaaggccg tcccacccgg cagctgacct
    10021 tcgtcaggca cgaacaccaa cgcgcgagtg cccacatatc ggaacttgcc gggcactcgc
    10081 ggcgagagcg gcaactccgg cgcgtccttc gcctcctcga gcccgcgcag agcctgacta
    10141 aataccagcg atagctcggc gcccgccgcg gcctcgccgc gcggggccgc aaaaaccacg
    10201 cgaaacgcgc cttcctgcgc cccgcgttgc tcaccaacgg gctgcaaagc acgccccgtg
    10261 ctggtggtag gtttgttcgc gctgctacac gtacacagca gcatcaccaa aagcatccaa
    10321 ccaaaccgcg acacccgcac agaacgccac agattgcggc ccccttccag cacaacccac
    10381 aagcgcactg cggtttgtgg cgcgaagcct gtgactcgcc gaaccctgca tctgccgcaa
    10441 gccggatggc ccgcccgcag gagacgggcg cgaaaacccg tccattcacc tcatcagcgc
    10501 ggtcattttg ctccatacaa gccccgagta ccgcgcaagc ggcacccttc aacgcctccc
    10561 gctctacggc gaagccccga ttcacggcga agccccgatt tacggcgaag ccccgaagcg
    10621 ctcacgcgcc tcggggctcc ctctcattca acccgagtct ggggctttgc tcgaaagagt
    10681 ccgctcactt ggcggcgcgc ggttgtgcct tctgctgaag aagccaactg aagtcggcgc
    10741 tgagctgaga cgcaaactgg taagttcccc cggccgtgct cggagcggct ttcgcaatac
    10801 ccgccgacga gtggcacccc atgcagctct cggtgctcaa caccaacttg ccggcggcgt
    10861 ccccacagtt cgcacccacc gagcggcaga agccctctgc gaaatcgagc ggcgtgtttc
    10921 ctccgttggt ggagggcacg tcataacctg gagagcccgc cgatccgtcc tcgttgacac
    10981 tcaggtgcac ctgctcgggt ttgcccgcgc cgtttgccga agaggcgccg ccctgaaagt
    11041 acgtctccat caccgcgttg atcaagaaca ccggcgtagg cttccctccg ctcttgttgg
    11101 tgatgttact tgggtaatcc tgaatatcgg ctggcggcgc gctcggatcg gtaggccatt
    11161 gggtattgat gagctcgtaa tactgcaaag ccgagccgag
//
LOCUS       DASTKU010000234         5281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_493288, whole genome shotgun sequence.
ACCESSION   DASTKU010000234 DASTKU010000000
VERSION     DASTKU010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493288"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VFQ61_08675"
     CDS             <1..999
                     /locus_tag="VFQ61_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9954566.1"
                     /translation="RVWASNPALLAKPEHELLGRTLLETLGDDVGRWHHEKVCLTLET
                     GLDQNYEYDLQLPAGIRHFACSSVAVPAPDEKGRAAVFWIRDVTDQIELRQKLLHSQR
                     LASIGTLAAGVAHEINNPLGYTLLNVERLANLVSEFSNEPGLRERMQLLATPLDMIRE
                     GMQRVERIVRELLDFSRADNPFEAVDIRHALQISIEAAKSELEARAQLALDYADTPEI
                     WGNERRLVQVFTNLLRNAAQAIPPGAVDQNTIRVRTSASGETVRIEISDTGVGIAKSD
                     LPRIFDPFFTTRATGTGLGLAICHRIVTSMDGEIQVESQLGQGSTFSIVLPRLSTH"
     gene            complement(1041..2504)
                     /locus_tag="VFQ61_08680"
     CDS             complement(1041..2504)
                     /locus_tag="VFQ61_08680"
                     /inference="COORDINATES: protein motif:HMM:NF020826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9954567.1"
                     /translation="MRIQRRHWLQAAAALCVTGFDPKTRSWIAGAQASSQPNPMPCIP
                     GLMGQLLLDSASVSPYADDFGHIVHRAPLAVLRPACLFDVVLAVRFARAARIPIAMRG
                     QGHCQLGEAQVQAGIVVDSSTLSTIHALSPSSATVDAGVTWSDLVLAGAEAGATPPVL
                     TDYLGLSVGGTLSVGGVGGAMHAHGLQADNVLALWVVTGKGDLVECSEAQQPELFRAV
                     LTGLGQFGLIVRAKLRMVPAPSQALVFNLYYDDIEAYVTDQTKLVSEHRFSYLEGQVT
                     KSADGLSYRYMIEAVQYFEPGDEPDAAALVADLAYDASTSPAPSAIPYLDFAFRLEPV
                     IQFLKQANLWGLPHPWLSLWVPASKAASYVQSIVANLSDFQTGQGPILFYPVWKPAVR
                     SPLYRLPEETVSFAFNILRTAIPGATDSVDNLLASNRALYDQLVALGGTRFPIGAIPF
                     SPSDWRAHFGADWPRFERLKRKYDPQHILTPGQRVFA"
     gene            complement(2733..4439)
                     /locus_tag="VFQ61_08685"
     CDS             complement(2733..4439)
                     /locus_tag="VFQ61_08685"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9954568.1"
                     /translation="MSRTQSLASCPWSRIAVIALFALGNAACSDSEPAPSRAPEVDQS
                     EALYAPDRLVEIAIEMDEAEWDALRDEGHSLLEFQQGTPVGFDYTYFSASVKVDDKPL
                     ERVAIRKKGALGSLSRFRPSLILDIDRNVEDQKVYGVNRLTLNNDRSDSAHARTCMAY
                     ELFTKAGLPASRCNLAHVTVNGHDLGTYSNVEPIRKPMLARHFADNDGNLYEGQEDAD
                     FTQEAYQRFQVKTNEDKNDKSDLARVAQAMEADDAHLVTELSKVIDLDHFRRFWAMET
                     LSGNWDSYSGNTNNFFTYHDPTTDKFYFMPWGPDASFTGASIIDSYNKTLTVYATGAI
                     SNRLYNIPSERKKFRDLLADLNDGVWDTPALLSRLYELQDISTDAWPGAVENLREYIS
                     TYSDRLRAELAEPAPEWKLGGIDLRDECAGQLGEASASFQFPFTSGELSPDPSVGAVQ
                     ATLKIGDKSIEAFWFGGVGRNPEGSDRQVGMQLFGAMPDGRIAILALSMPPQGFAPGT
                     QPFYNFETVGYVVIGQGEEFKFAGLVTEGSIEFDEASMEAGADVAGSFHGLVYQTGCV
                     DL"
     gene            complement(4515..>5281)
                     /locus_tag="VFQ61_08690"
     CDS             complement(4515..>5281)
                     /locus_tag="VFQ61_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954569.1"
                     /translation="IKFSPTEFLDAVEKQVIEPMRSVQKLLDVHPYVTRLYTTMSAEE
                     MTEDPLFTFNPDLQDLNNIHTADRVIECSPELTQFEAPWRVELPQGGVVRGTGAQAQS
                     RTWPSEVATLPANLRVLKLSSSGEGKVVLDNTDEIEAVTQGLPQSTGGSSQGGSAAGG
                     SSNSGASPGTGGAATGGKTALPNGGSGNRTSTGTATGTGATGGSDKEIWPPKTSGSSG
                     CTVAAVSAPGAGAEWLVAGLAMGLRALRRRARRDPK"
BASE COUNT          981 a         1660 c         1625 g         1015 t
ORIGIN      
        1 cgggtttggg ctagcaatcc cgcgctgctc gccaagccag agcacgagct actcggtcgc
       61 acgctgctcg agacgttggg tgacgacgtc gggcgctggc accacgaaaa ggtctgtctc
      121 acgctcgaga ccgggctcga ccaaaactac gaatatgatc tgcagctccc tgcgggcatc
      181 cggcacttcg cgtgcagttc ggtcgccgtg cccgcgccgg atgagaaggg gcgggcggcc
      241 gtgttctgga ttcgtgacgt aaccgatcag atcgagttgc gccagaaact cctccactcc
      301 caacggctgg cctcgattgg cacgttggcg gcgggtgtgg cccacgaaat caacaatccg
      361 ctcggctata cgctgctcaa tgtcgagcga ctcgcgaacc tagtcagcga gttttcgaac
      421 gaacctggcc tgcgcgagcg catgcaactg ttggccaccc cgctcgacat gatccgggag
      481 ggcatgcagc gtgtcgaacg gatcgtgcga gagctcttgg acttctcgcg tgcggacaat
      541 cctttcgaag ccgtggacat ccgtcatgcc ttgcagatct cgatcgaggc ggccaaatcc
      601 gagctcgagg cacgtgcgca gctcgcactc gattacgccg ataccccaga aatatggggt
      661 aatgaacggc ggctcgttca ggtattcacc aatctgctcc gcaacgccgc gcaagccatc
      721 ccccccggcg ccgtagatca gaatacgatt cgcgtgcgca catccgcttc gggtgaaacg
      781 gtgaggatcg agatctcgga taccggggtg gggatcgcga agtctgactt gcctcggatc
      841 ttcgatccgt tcttcaccac ccgcgccacc ggtaccggtc tgggcttggc gatttgccat
      901 cgcatcgtga cctcgatgga tggagagatt caggtcgaga gccaactggg ccagggcagc
      961 acgttttcca tcgtgctgcc ccggctctcc acccactagc cgtcggggtt ccgcgtagct
     1021 tgtccgggcc aggctgcgga ctaggcgaac acgcgttgcc cgggggtgag gatgtgttgt
     1081 gggtcgtatt tgcgtttgag ccgctcgaac cgaggccagt ccgcgccgaa gtgggcgcgc
     1141 caatcgctcg gggagaaggg aatcgccccg attgggaagc gcgttccgcc caacgcgacg
     1201 agctgatcat agagcgcgcg gttagaggcc agcagattgt ccacgctatc cgtggcgccc
     1261 gggatcgccg tgcgtaggat gttgaaagcg aagcttaccg tctcttcggg caagcgataa
     1321 agcgggctcc tcaccgcggg cttccaaacg ggatagaaca ggattgggcc ctggccggtt
     1381 tggaaatcgc tcaaattggc tacgatggat tgcacataac tcgcggcttt gctggccggc
     1441 acccagagac tcagccaagg gtgaggcaag ccccagaggt ttgcctgctt gagaaactga
     1501 atcaccggtt cgagccggaa cgcgaaatcc aaatagggaa tcgcgctcgg ggcggggctc
     1561 gtactcgcgt cataggccaa gtccgcgacc agggccgcgg catcgggttc atctccaggc
     1621 tcgaagtact ggaccgcctc gatcatgtag cgatagctga gcccgtcggc gctcttcgtg
     1681 acttggcctt cgaggtagct gaagcgatgt tcggagacga gcttcgtttg atcggtgacg
     1741 taggcttcga tatcgtcgta gtacagattg aacaccagcg cctggctcgg cgcgggaacc
     1801 atgcgtagct ttgccctcac gatcaggccg aattggccga gaccggtgag aaccgcccgg
     1861 aacaactcgg gctgctgggc ctccgagcac tccacgaggt cccctttgcc cgtcaccacc
     1921 caaagtgcga gcacgttgtc ggcctggagc ccatgcgcat gcatggctcc gccgacacct
     1981 ccgacggaca aggtgcctcc cacggacaga cccagataat cggtcagcac cggtggcgtg
     2041 gcgccggcct cggccccggc caagaccaga tcactccagg tcacgcccgc atccacggtg
     2101 gcgctggagg ggctcagcgc gtgaatggtg gagagcgtac tcgaatccac cacgatgccg
     2161 gcttggactt gggcctcacc tagttgacaa tgcccttgtc cgcgcatggc aattggaatg
     2221 cgcgccgccc gcgcgaagcg gaccgccagc accacgtcga acaggcatgc tggccgcaac
     2281 accgcgagcg gcgcgcgatg cacgatgtga ccgaagtcgt ccgcgtaggg agacacgctc
     2341 gcgctgtcca atagcagctg tcccatcaag ccggggatgc acggcatggg attgggctgc
     2401 gagctagcct gggcgcccgc gatccaggag cgcgtcttcg gatcgaagcc ggtgacgcac
     2461 agtgcagccg cagcctggag ccagtgtctc cgctgaattc gcacaggtaa atttccctct
     2521 cgcatacgac ctcctttgat gctcagtgtt gctgctggcc ttccgagccg aagctccgcc
     2581 cgaaccagca gcacgcactc atagcatccc cccgggtgag aacgcagtcg ggaaacggcg
     2641 ccgcctgcac gcgacgccgt catcattggg agtcaatcta ggtcgaggtc cgtcgctgtg
     2701 ggaggcttgc gcccgtcagc gccagcctcg aatcacaagt cgacacaacc ggtttgatag
     2761 accaagccgt ggaaagaccc cgccacgtcg gcacccgcct ccatactcgc ttcgtcgaac
     2821 tcgatgctgc cttcggtgac gagccccgcg aacttgaact cctcaccttg accgatcacg
     2881 acgtacccga ctgtttcgaa gttgtaaaag ggctgtgtcc caggggcgaa gccctgaggt
     2941 ggcatcgaga gagccaggat ggcgatgcgc ccgtctggca tcgccccaaa cagctgcatg
     3001 cccacttgtc gatccgatcc ctcggggttt cgtcccaccc ctccaaacca gaaagcctcg
     3061 atactcttgt ccccaatctt gagggtggcc tgcacggcgc cgacgctagg atcgggcgag
     3121 agctcgccgc tcgtgaacgg gaattggaag ctcgcagacg cctcgcccag ttgccccgcg
     3181 cactcatctc tcaaatcgat gccgcccaac ttccattctg gcgcgggctc ggccagctcg
     3241 gcacgtagcc gatccgaata ggtggagatg tattctctga gattttcgac cgcgccgggc
     3301 caggcatcgg tcgagatgtc ttgcagctcg taaagacggg acagcagcgc gggtgtgtcc
     3361 caaacaccgt cgttcaagtc cgccaacaag tcgcggaact tcttgcgctc gctcgggatg
     3421 ttgtagaggc gattcgagat agcgcccgtc gcgtagacgg tgagcgtctt gttgtaagag
     3481 tcgatgatgc tcgcccccgt gaacgacgca tccggtcccc agggcatgaa gtagaattta
     3541 tcggtcgtgg ggtcgtgata cgtgaagaag ttgttggtat tcccgctgta agagtcccag
     3601 ttgccgctca gtgtctccat tgcccagaag cggcggaaat ggtcgaggtc gataaccttc
     3661 gagagttcgg tgacgaggtg cgcgtcgtcc gcttccatgg cttgggccac gcgcgccaaa
     3721 tcactcttgt cgttcttgtc ctcgttggtt ttcacctgga agcgttgata cgcctcttgc
     3781 gtgaagtcgg cgtcctcttg gccctcgtat aggttgccgt cgttgtccgc gaagtggcgc
     3841 gccagcatgg gcttacggat cggctcgacg ttgctgtacg tcccgagatc gtggccgttc
     3901 accgtcacgt gagccaagtt gcagcgcgac gcgggtaacc cggccttcgt gaacaactcg
     3961 taagccatac aggtccgagc atgcgccgaa tccgagcgat cgttgttcaa ggtcagacgg
     4021 ttcacaccgt aaaccttttg gtcctcgacg ttgcggtcga tgtcgaggat gagggaggga
     4081 cgaaagcgcg aaagcgaacc cagagcaccc ttcttgcgga ttgcgacccg ttctagcggc
     4141 ttgtcatcca ccttcacgct ggccgaaaag taggtgtaat cgaacccgac cggggtgccc
     4201 tgctggaact cgagcagact gtgaccctcg tcccggagcg cgtcccattc ggcctcatcc
     4261 atttcgatcg cgatttcgac cagccgatca ggggcatata gcgcctccga ctggtcgact
     4321 tcaggcgcgc gggacggagc cggttccgag tcggagcagg cggcgtttcc caacgcgaac
     4381 agggcgatca cggcaatgcg agaccaggga catgaagcaa ggctttgggt gcgcgacatc
     4441 ggtcgatcaa tgtcgtcgac ttcgcggact cgtcacagaa acgtcgcgca ccctgccgcg
     4501 ccctactcag aggtttactt gggatcgcgg cgcgctcgtc ggcggagtgc gcggagcccc
     4561 atcgcaagcc cggcaacgag ccattctgca ccagcccccg gtgctgaaac cgccgcaacc
     4621 gtgcagccag agctgccgct tgtcttcggt ggccaaattt ccttgtccga gccccctgtc
     4681 gcgccggttc cggtcgcagt gcccgtcgag gttcgattgc ccgagccgcc attggggagg
     4741 gccgtcttgc cgcccgttgc cgcgccacct gtaccaggtg aggcacccga attcgagctg
     4801 cctccggccg cactgccgcc ctgactgctg cccccggtag attgcggcag accctgcgtg
     4861 accgcctcga tttcgtcggt gttgtcgagc acgaccttgc cctccccaga gctcgacagc
     4921 ttcaagaccc ggaggtttgc cggcaacgtc gccacttcgc tcggccaggt gcggctttgg
     4981 gcttgtgcgc ccgtaccgcg gaccaccccg ccttgcggca gttccacgcg ccagggcgct
     5041 tcgaactgcg tgagttcggg cgagcactcg atcacccggt cggcggtgtg gatattattg
     5101 aggtcctgca gatccggatt gaacgtaaac aacggatctt cggtcatctc ttccgcggac
     5161 atcgtggtgt agagtcgcgt gacataaggg tgcacgtcga gcagcttttg tacgctgcgc
     5221 atcggctcaa tcacctgctt ttcgaccgca tccaagaact ccgtggggga gaacttgatt
     5281 t
//
LOCUS       DASTKU010000235         4371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_715278, whole genome shotgun sequence.
ACCESSION   DASTKU010000235 DASTKU010000000
VERSION     DASTKU010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715278"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFQ61_08695"
     CDS             complement(<1..319)
                     /locus_tag="VFQ61_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954570.1"
                     /translation="MSTETGQLQSGQDDSIALTLAGFVIGMDEHDRAAASQAFEAALT
                     LSPSSAFTYLLGSPVLGWGGDAERAIEWAERALRLSPLDPWNFFCCHGLVLGHFLLGR
                     YREA"
     gene            complement(333..1579)
                     /locus_tag="VFQ61_08700"
     CDS             complement(join(333..1312,1312..1579))
                     /locus_tag="VFQ61_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611749.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET9954571.1"
                     /translation="MEREGVHKCPYAAEFRQEFVDLARSGRSVKELAEEFEPSEQTIR
                     NWLAEADAESGKRKDLLTSAEREELVKLRPENKQLKTEREILAKANGLVRKGGRYETQ
                     EVFGFVAAHQAMFPVRAMCRVFGVSPSGFYAWHGREPSARALEDARLTARIRYYHARS
                     KGRYGAPNIHADLREEGTRVGQKRVARLMRAEGLQGACFRKWVTTTLRDADAQAAPDL
                     VERRFVAEAPDQLWVADITYIPTWAGFLYLAVVLDAFSRRIVGWSMAEHLRTELVLAA
                     LNMALGQRRAKGVVHHSDQGTQYTSIEFGLRCKEARVRPSMGTVGDAYDNAMCESFFA
                     TLERELLARRRFATKAEARMAIFEFIEGWYNPTRRHSGLGRISPVEFERRHSTHNEHA
                     VTHRPARSVACEGPAHGPVDNRL"
     gene            complement(1720..1989)
                     /locus_tag="VFQ61_08705"
     CDS             complement(1720..1989)
                     /locus_tag="VFQ61_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954572.1"
                     /translation="MSDDLIFMLAVAIGVGVATGCVARLAKRFAGVQSSPIELGAFAG
                     ALIALLVFGIRSRDVFLIGSIAALVIGGFFLIKILRRVMGADKGK"
     gene            complement(2003..>4371)
                     /locus_tag="VFQ61_08710"
     CDS             complement(2003..>4371)
                     /locus_tag="VFQ61_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HET9954573.1"
                     /translation="STTDALGRTTAYQYDAAGNTTQMTLLSGTANAVSWSYTYEPIYN
                     QVATTTDPLNHTTTYEYDSLGNRIRVTDPLNHVTQMTYTPSGQLSTVTDALNHTTTLS
                     YVGGDLVSIADPLGRTVSFGLDAVGRVVSVTDPLGNRTETAYDALDRVTTRTDALGQQ
                     TAYGYDAKGNLTSFTDARSNVTAFAYDARNRQISKTDALTQVETYQYDAAGNLAFVTD
                     RKGQVRGFTYDLLGRRTQVGFGATSTTSPVYDSTIAYTYDAANRLTQIADSANGTITR
                     TYDDRFDTLAQETTPQGTVAYTYDAAGRRATMTPSGGTQAAYAYDEAGRLTQITQGAS
                     TVQFAYDAANRRSTLTLANGISVTYAYDNASQVTSIAYRDASANLLGDLTYAYDNAGR
                     RTAMGGSFARANLPAALASASYDANNRVSAWGGSSLSHDANGNLLGFQGDTYAWNSRD
                     QLSGISGTNTASFAYDAVGRRQGKTIGGTSTQFLYDGVNPIQELSGTTVTANVLAGLG
                     IDEYFLRTEGSSNQHYLTDALGSTMRLTDNSAVKLVDYTYEPYGKASADAASSNALQY
                     TGRENDGTGLNYYRARYQSPVLGAFISEDPIGLAGGENVYAYAGNDPIALLDPLGLWS
                     WAGAAGGAIGGAIGGGVGGAIVGSISGPGIVAATVAGAVAGAVGGAITGGMNESGGSA
                     GAFAGGLGGAIAGSSGKLPGIVGGAVGGAIGGAVNSNNPAVTGAVGAVAGAIGGGIAG
                     GLEKPNIASAVNGAVAGAVAGFISGSATTMIINASAANKPVPNWCYYP"
BASE COUNT          749 a         1417 c         1356 g          849 t
ORIGIN      
        1 ccgcttcccg atagcgccct aacaagaagt gtccaagcac gaggccgtgg caacagaaaa
       61 aattccaagg gtcgagtgga ctcagacgaa gcgcgcgctc agcccactcg atggcgcgct
      121 cagcatcacc gccccagccg agtaccgggc tgcctaggag ataagtgaaa gcggaggacg
      181 gactgagggt aagtgcagct tcgaacgcct gggaagctgc ggcccggtca tgctcatcca
      241 tgccgatgac gaatcccgcg agcgtcagcg caatgctgtc gtcctggccg ctctggagtt
      301 gacccgtttc agtggacacg ttggcgcttg agtcacaagc ggttatccac aggcccgtgc
      361 gcggggcctt cacaagcaac cgagcgcgcg ggcctgtggg taaccgcgtg ctcgttatgt
      421 gttgaatgac gccgctcgaa ctcgaccggc gagattctgc caagaccgga gtggcgtcgc
      481 gtcgggttgt accagccttc gatgaactcg aagatcgcca ttcgcgcttc ggctttcgtc
      541 gcgaagcgcc ggcgcgcgag caactcgcgc tcgagagtgg cgaagaagct ctcgcacatc
      601 gcgttgtcgt aggcgtcgcc cacggttccc atcgagggcc gaaccctcgc ctctttgcag
      661 cgcagcccga actcgatcga ggtgtactgc gtgccctggt cggagtgatg caccacccct
      721 ttcgcgcggc gctgtcccag agccatgttg agcgcggcga gcacgagctc ggtgcgcagg
      781 tgctcggcca tgctccagcc gacgattctg cggctgaagg cgtccaggac caccgcgagg
      841 tagaggaagc cggcccaagt ggggatgtag gtgatgtcgg ccacccacag ctgatcgggt
      901 gcttcggcaa caaagcgccg ctccacaaga tccggtgccg cctgagcgtc ggcatcgcgc
      961 agcgtcgtcg tcacccactt gcggaaacac gctccttgca ggccttctgc tctcatgagc
     1021 ctcgccacgc gcttctgacc cacccgcgta ccttcctcgc gcagatcggc atggatgttc
     1081 ggtgccccgt agcgcccctt cgagcgagcg tggtagtagc gaatccgcgc cgtgagtctc
     1141 gcatcctcaa gtgctcgagc cgacggctcg cgcccgtgcc atgcgtaaaa accgctcggg
     1201 gagacgccga acacgcggca catcgctctt accggaaaca tggcctgatg cgccgctacg
     1261 aacccgaaga cttcttgggt ttcgtatcgg cctcccttgc gaaccaggcc gtggcctttg
     1321 ccaggatttc gcgctcggtc ttgagctgct tgttctcagg gcgcagcttc accagctcct
     1381 cgcgctcggc gctcgtcagc agatcctttc gcttgccgct ttccgcgtcc gcttccgcca
     1441 gccaattacg aatcgtctgc tccgagggct cgaactcctc ggcgagctcc ttcacgctgc
     1501 ggccagaacg agcgagatca acgaactctt gccggaactc agccgcatat gggcatttat
     1561 gaactccctc cctttccaaa ggatgagatg tccacaaagg cgggtcaact ccactctctt
     1621 cccgtgtact gcagcgagtt ggctgaatcc gcgccgagcg tcgtcgcctc cccgtacggc
     1681 gtgtaggcgt gaagattctt ttgcgcgatg ttcccgggct cacttcccct tatctgcgcc
     1741 cataacccgt cggaggatct tgataaggaa gaatccgcca atgacgagag cagcgatgct
     1801 gccaattaaa aagacatccc gacttcgaat gccaaatacg agcaaagcga tcaaggctcc
     1861 cgcgaacgca ccgagttcta ttggcgaaga ctgaaccccg gcgaaccgct tcgctaggcg
     1921 agcgacgcaa cctgtggcta caccgacgcc gatggccacc gcaagcatga agatcagatc
     1981 gtctgacatt gagggaaggg atctatgggt agtagcacca attgggcacc ggcttgttgg
     2041 cagctgacgc gttaatgatc atggtcgtag ccgagccgga aatgaagcca gcaacggcac
     2101 ctgctaccgc gccatttacc gcgctcgcaa tgtttggctt ctcgaggccg ccggctatgc
     2161 cgccaccgat tgcaccagcc acagcaccga cggcacctgt cacggcagga ttgttggaat
     2221 tgactgctcc gcctatcgct ccgccaacgg ctccgccgac gataccgggc agctttccac
     2281 tggaaccagc aatagcccct ccgagaccac cagcgaatgc acctgcgctg cctccgcttt
     2341 cattcatgcc gcccgtaatc gcgccgccca ctgctccggc gacagctccg gcgaccgttg
     2401 cagcgacgat gccaggccca cttatagagc caactatcgc accacctacg ccaccaccta
     2461 ttgcaccacc tattgccccg ccagcggccc cggcccaact ccacaaccca agaggatcaa
     2521 gcaaagctat tgggtcgttt ccggcgtagg cgtaaacgtt ctccccgccc gcgagcccaa
     2581 tcggatcctc cgaaataaat gctccgagca ccggactctg atacctcgcc cggtagtagt
     2641 tcagccccgt cccgtcgttc tccctacccg tgtactgcaa agcgttactc gacgccgcat
     2701 ccgctgacgc cttgccgtaa ggctcgtagg tgtagtccac cagcttcacc gccgagttgt
     2761 ccgtcaagcg catcgtcgag cccagagcgt cggtgaggta atgctgattg ctgctccctt
     2821 ccgtgcgaag gaagtattca tcgataccga ggcccgccag cacgttcgcc gtcactgtcg
     2881 tgccagagag ctcctgtatc ggattcactc cgtcgtatag gaattgcgtt gacgttccgc
     2941 cgatggtctt cccctgccgc ctccccaccg cgtcgtacgc aaagctcgcc gtgttcgtcc
     3001 cgctgatgcc ggagagctgg tcgcggctgt tccaggcgta cgtgtcgcct tggaacccaa
     3061 gcaggttgcc gttggcgtcg tggctgaggc ttgagccacc ccaggcgctg acgcggttgt
     3121 tcgcgtcgta gctcgccgag gcgagcgccg cggggaggtt cgcgcgcgcg aagctcccgc
     3181 ccatcgccgt gcgccgcccg gcgttgtcgt aggcgtaggt gagatcgccc aggaggttcg
     3241 ccgaggcatc gcggtaggcg atcgaggtca cctggctcgc gttgtcgtag gcgtaggtga
     3301 cgctgatgcc gttggcgagg gtgagcgtgc tcctgcggtt cgccgcgtcg taggcgaact
     3361 gcaccgtgct cgcgccttgc gtgatctgcg tgagccgccc cgcctcgtcg taggcgtagg
     3421 cggcctgcgt gccgcccgag ggcgtcatcg tcgcccttcg ccccgcggcg tcgtaggtgt
     3481 aggcgaccgt accctggggc gtcgtctcct gggcgagcgt gtcgaagcga tcgtcgtagg
     3541 tccgggtgat ggtgccgttc gccgagtccg cgatctgcgt caggcggttc gccgcgtcgt
     3601 aggtgtacgc gatggtgctg tcgtagaccg ggctcgtggt cgaggtggcg ccgaagccca
     3661 cttgggttcg cctaccgagc agatcgtaag taaagccccg cacctgcccc ttgcggtcgg
     3721 taacgaaggc gaggttgccc gcggcgtcgt actggtaggt ctcgacctgg gtgagcgcgt
     3781 cggtcttgct gatctgccta ttacgcgcgt cgtaggcgaa ggcggtgacg ttgctgcgcg
     3841 cgtcggtgaa gctcgtgagg ttgcccttcg cgtcgtagcc gtaggcggtc tgctggccga
     3901 gggcgtcggt gcgggtcgtc acccggtcga gcgcgtcgta ggcagtctcg gtgcggttgc
     3961 cgagcgggtc cgtgacgctg acaacccgcc ccaccgcatc cagcccgaag ctcacggttc
     4021 gacccagcgg atcggcgatc gatacgaggt ctccgccgac ataggaaagg gtggtcgtgt
     4081 ggttcagcgc gtccgtaacg gtcgaaagct ggccggaggg cgtgtacgtc atctgcgtga
     4141 cgtggttcag cgggtcagtc actcgaatcc ggttgccgag cgagtcgtac tcgtaggtgg
     4201 tcgtatggtt gagaggatcg gtcgtcgtgg cgacctggtt atagatcggc tcgtaggtgt
     4261 agctccatga cacggcattg gcggtgccgc tcaggagcgt catctgcgtg gtattgccgg
     4321 cggcatcgta ttggtacgcg gtcgttcgcc ccagcgcgtc ggtcgtgctc g
//
LOCUS       DASTKU010000236         3258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1385582, whole genome shotgun sequence.
ACCESSION   DASTKU010000236 DASTKU010000000
VERSION     DASTKU010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385582"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFQ61_08715"
     CDS             <1..336
                     /locus_tag="VFQ61_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954574.1"
                     /translation="VTLHLSRPAAATALRYSVQTLTNVSGYSAGAPTLEAGVIGGSKR
                     VRSEPITSASAGSSTGDSSWGFAGPKQEVSLSLPDVGSDVVIRLTPSSCMGLCPPPQA
                     LWIDDLRVE"
     gene            424..966
                     /locus_tag="VFQ61_08720"
     CDS             424..966
                     /locus_tag="VFQ61_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954575.1"
                     /translation="MSLTESLLLVLAILGSAGIAWGMTRWSMNKHAERNARVAVSDAA
                     LMEVARRLGLTFGAGPAYEHPFIGPIAAFGAARGVLDGIAVHVGVDMGSEGDTARTEL
                     RAELPGGYDAALLPRYALNCERYTLERGPKTLTLYPVVRKTGSSQFIVYELETEPATI
                     IALLRELVELARTASERTRY"
     gene            1058..1849
                     /locus_tag="VFQ61_08725"
     CDS             1058..1849
                     /locus_tag="VFQ61_08725"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HET9954576.1"
                     /translation="MTDDAHGRGAGAPRAFTDENVPAPTHAERARTLIARHQTGTLGT
                     LAVEPAGYPYGSFVPYAVVEGEPVFLISRLAEHTRNLAADARASLLVHETGKADPLAN
                     ARVTLLGRAQRLEKGPSSAREQFLATHPHARHYADYSDFEFWRLSVESLRYIGGYGRM
                     SWIQPDDFRAAEPDPLAESATHILSHMNNDHPDALRLYSRAFTRAVDAESAVMTAIDR
                     YGFELTITTPSGSGPARIAFELPLNSAEDAHDTLIALVHVAELLQ"
     gene            complement(1899..>3258)
                     /locus_tag="VFQ61_08730"
     CDS             complement(1899..>3258)
                     /locus_tag="VFQ61_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9954577.1"
                     /translation="IAWFEALGLLAVSAALAVARRRRFPIPESARLPLLLLLALLAFN
                     HVANILEQLGATWADSVADDLAMATPLVWGLFLVEVGRSFLQRQVDVHADQLHFLVER
                     VPASVAWLDAELRVQAVSRVWESQFPNSCGHPLREVMPAQLSELTRAIDECLHAGPAE
                     KHGEDQSRDSAGRLRYYRWALRRVDPAEEGRVGALLILEETTEAVEAEAERALAAEQL
                     ASTQRAADLGQLAAGAAHDLNNMLQVIHNANEELSELPGAALASSDIRQAVQSACAMT
                     RTLLRLGVQKKQTLTPLDLTALLAQVEGLLKIALGRKHRLIVQKPRQPILIAGDADRL
                     EHAILNLVVNARDASPAGGSIYLTLEVIEGVARLTVKDEGEGIPLEIQGQLFRPFFTT
                     KGARGTGLGLVSVRSTVTQHGGTVTVQSWPGAGSEFEMRIPLTRAHALESTLPPSASC
                     SD"
BASE COUNT          574 a         1032 c         1052 g          600 t
ORIGIN      
        1 gtgactctgc atctgtctcg cccggccgcc gcaactgccc tgcgctactc cgtacaaacg
       61 ttgaccaatg tgagtgggta cagcgcgggc gcacccactc tggaggcggg ggtgattggt
      121 gggagcaagc gggtacgctc cgagccgatc acgagcgcga gcgcgggcag ctcgacgggc
      181 gacagctcat ggggctttgc cgggccgaaa caggaggtgt ctttgtcgct cccggacgta
      241 ggctccgacg tggtgatccg gcttacgcca agttcgtgca tgggcctgtg tccgccgccc
      301 caggcgctgt ggatcgacga cctccgcgtc gagtgagctc gatcaagcgc gctggatttc
      361 tctcggcgcg ctcgcctgaa aaacaacagc ttgtaggcga gcggtggtaa gcgtcccggc
      421 agcgtgtcat tgacggagtc tttgcttttg gtgctggcga tcctcgggtc cgcggggatc
      481 gcatggggta tgacccgctg gtccatgaac aagcacgcgg agcgaaacgc ccgcgttgcg
      541 gtgagcgatg cggccttgat ggaggtggct cggcgtttgg ggctcacgtt cggagccgga
      601 cccgcatacg aacacccgtt catcggtccg attgcggcgt tcggcgcggc acgtggggtt
      661 ctcgacggaa ttgccgtcca cgtaggcgtg gacatggggt ccgaaggaga caccgcgagg
      721 accgagcttc gcgctgagtt gcccggcggt tacgacgcgg cgctgctgcc gcgttatgcg
      781 ctcaattgcg agcgctatac gctcgaacgt ggtcccaaga cgctcaccct atatcccgtg
      841 gtccggaaaa ccggatcgtc tcagttcatt gtttacgagc tggaaaccga gcctgccacg
      901 atcattgccc tgctgcgaga gctggtggag ctggcgcgga ccgcatccga gcgtacccgg
      961 tactgagagc agcgcggcgt agtccggcgg cagcgggcgt gggcggtagc tccgataggg
     1021 ttcgctcggc tgcagatatg ttctaagctt ggccgcgatg actgatgatg cccacgggcg
     1081 cggagcgggg gcgccccgcg ccttcaccga cgaaaatgtc cctgcaccga cccatgccga
     1141 gcgcgctcgc acgctgatcg cacgtcacca aaccggcacg ctcggcacgc tggccgtgga
     1201 accagcaggc tatccgtatg gttccttcgt cccctatgcg gtggtggaag gcgagcctgt
     1261 atttttgata agtcgactgg cggagcatac tcgaaatcta gccgcggacg cgcgagcgtc
     1321 tttgctcgtg cacgagaccg gcaaggcgga ccctttggcc aacgcccgcg tgactctgtt
     1381 ggggcgtgcg caaagactag agaaggggcc gagctcggcg cgtgagcagt tcttggccac
     1441 tcacccgcac gcgcggcatt acgcggacta ttcggacttc gagttttggc ggctctccgt
     1501 cgagtctttg cgctatatcg gcggctacgg ccgaatgtct tggattcagc cggacgactt
     1561 ccgcgccgcg gagcccgatc cgcttgctga gtcggcgacg cacattctaa gtcacatgaa
     1621 caacgaccac ccggacgcgc tgcggcttta tagccgtgcg ttcacgcgcg ccgtcgacgc
     1681 agagagcgca gtcatgaccg cgatcgaccg gtatggcttc gagctcacga tcaccactcc
     1741 ttccgggagc gggcccgctc gcatcgcctt cgagttacct ttgaacagcg ccgaagacgc
     1801 ccacgatacg ctgatagcgc tggttcacgt cgctgagcta cttcagtgag cctcggaatc
     1861 gcgaagcgat ccacgagcgc agatgacatg cgttgggact agtccgagca actcgctgat
     1921 ggcggcagcg tggactcgag tgcgtgcgcg cgcgtcaacg gaatgcgcat ttcgaactcg
     1981 ctgcccgcgc ccggccaaga ttgtacggtc accgtgcccc catgttgggt cacggtgctg
     2041 cggacactga ccagcccaag cccagttccg cgcgcgccct tcgtcgtgaa gaacggccgg
     2101 aagagttggc cctgaatctc cagcgggatc ccttcgcctt cgtccttgac ggtgaggcgg
     2161 gcaacgcctt cgatcacctc taaagtcaag taaatcgagc cgcccgccgg gctcgcgtca
     2221 cgtgcattca cgaccagatt gaggatcgca tgctccagcc gatcagcgtc gccggcaatc
     2281 aggatgggtt gccgcggctt ctgcacgatc aagcggtgct tgcggccgag cgcgatcttc
     2341 aacaagcctt cgacttgagc gagtagggcc gtgagatcca gcggagtcag ggtttgcttc
     2401 ttctggacgc ccaggcgtaa aagcgttcga gtcatggcgc aagccgattg tacggcttgt
     2461 cgaatatcgg atgaagcgag tgccgctccg ggcaattcgg acagctcctc gttggcgttg
     2521 tgaatcactt gcagcatgtt gttcagatcg tgggccgcgc ccgcggcgag ctggcccaaa
     2581 tctgccgcgc gctgagtgct ggcgagttgc tcggccgcga gcgcgcgctc agcctcggct
     2641 tccacggcct cggtggtctc ttccaagatg agcaacgctc ccacgcgccc ttcctccgcc
     2701 ggatccacgc gtctaagcgc ccaacgatag tagcgcagcc gtccggcgga atccctcgat
     2761 tgatcttctc cgtgtttctc ggcgggcccc gcgtgtaggc attcatcgat agcgcgcgtt
     2821 agctcgctca gctgcgcggg catcacttcc cggagcgggt gtccgcagga gttggggaac
     2881 tggctttccc acacccggct gaccgcctgc acgcggagct ccgcgtccaa ccaagctacg
     2941 gaggcgggga cccgctccac caagaagtgc agttgatcgg cgtgcacgtc gacttgccgc
     3001 tgcaagaagc tgcgccccac ctcgaccaga aagagccccc acacgagtgg cgtcgccatg
     3061 gccaagtcgt cagctaccga atcggcccag gttgcgccca gttgctcgag gatgttcgcg
     3121 acgtgattga acgccaaaag cgcgagtaac aggagcaacg gtaaacgggc ggactccggg
     3181 attggaaagc gccgtcgacg cgccaccgcc agcgccgcgg acaccgcgag caagcccaac
     3241 gcttcgaacc aggcaatc
//
LOCUS       DASTKU010000237         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_268988, whole genome shotgun sequence.
ACCESSION   DASTKU010000237 DASTKU010000000
VERSION     DASTKU010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268988"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VFQ61_08735"
     CDS             complement(<1..672)
                     /locus_tag="VFQ61_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954578.1"
                     /translation="MLFRNNTVLPYLSLLSILVACGGNDDKPSQGCQLGDSSGCEGGQ
                     ICEQVENSQPACFAPLLLRGRVLDSETTKGIASARVVARDVNGELVSRDVAISDADGR
                     YALSIPAPRKQDGTPILTEALLRADAKAHATFPSGLRVALPVDTSAPRKEAAAWVVEN
                     GSTDISLDPVTGSESLGSVSGRVEAAAAPGALVVAGEKSSIAGRDGTFEIFDVAAGAI
                     EVRGYK"
     gene            complement(814..1626)
                     /locus_tag="VFQ61_08740"
     CDS             complement(814..1626)
                     /locus_tag="VFQ61_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954579.1"
                     /translation="MRKTILLASAVLYLPFFGCGPDTCKTGFIRQGDLCVEYGAPEPP
                     SSNGGSTGNGGSNAAGDGSDAGGTATASGGKTPANAGGAATGGKASSGGKNANGGDTV
                     ASNPGGADAGGTSQSDGGATSEPPDTTDVAFAELCTMHTDCAGTTNYCAFSAFEPAYC
                     SISGCDADPSICPPDWSCFNVGDFVPGEPWVCVKPPPATNGNGEMGSLCNSKTDCFGA
                     TNYCAYSPTEPAYCSVSGCDADPALCPTGYMCLNVGDFVPGEPWVCVKAPAM"
     gene            complement(1914..>3206)
                     /locus_tag="VFQ61_08745"
     CDS             complement(1914..>3206)
                     /locus_tag="VFQ61_08745"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET9954580.1"
                     /translation="QAEKGSGSNSRRAVALADLFARATRPEAEFLIRLISGELHQGAL
                     EGVVIDAVASAFSAPTDRVRRAVMLTGSLAKVGETLGRDGLSALAAFELELFRPIQPM
                     LADSAADVEDALQNLGEAAFEYKVDGARVQIHKDGDRVEVYSRSLNRVTAAVPEIVER
                     VSALPARKLVLDGEAIALSSERRPLPFQVTMRRFGRKLDVEAMRAELPLDVFFFDLLR
                     VDDQTLIDASWQERAQALQSLSPEDCRVSRLITKQADEADAFVSRALAEGYEGVMAKS
                     LSAPYAAGRRGSEWLKLKPVHTLDLVVLAAEWGSGRRTGYLSNLHLGARDPDTGAFAM
                     IGKTFKGMTDATLTMQTQALLAREIGREGHVVHVRPELVVEIAFNDVQVSAQYACGVV
                     LRFARLKRYRDDKTAEQADTIERVRSLLPAKSLRHQGR"
BASE COUNT          583 a         1099 c          936 g          588 t
ORIGIN      
        1 tttgtagccg cgcacctcga tcgcgccggc ggccacatcg aagatctcga acgtaccgtc
       61 tcgccccgcg atggagctct tttcgcctgc aaccaccagc gcgccggggg cggccgcggc
      121 ttcgactcga ccgctcaccg agcccaagga ctcgctgcca gtcacgggat ccaaggatat
      181 gtcggtcgag ccattctcta cgacccaagc cgcagcttcc ttgcgcggag cgctggtatc
      241 caccggtaag gccacccgca acccgctggg gaaggtcgcg tgtgctttcg cgtctgcgcg
      301 caacagcgcc tcggtcaata tgggagtccc gtcttgtttc cgaggggccg ggatactcaa
      361 tgcatagcgc ccgtcggcgt cactgattgc tacgtctcgt gacaccaact caccattcac
      421 gtcgcgcgcc acgacccgag cgctcgcgat ccccttcgtg gtttcactat cgagcacgcg
      481 cccgcgcagc agcagcggag caaagcaggc gggttgcgag ttctctactt gctcgcaaat
      541 ctggcccccc tcacagccac tggagtctcc caactgacag ccctgactgg gcttgtcgtc
      601 gttgccgcca cacgcgacga ggatcgacag gagactcaag taaggtagga ccgtattgtt
      661 tcggaacaac atggtgaatc agtgggcgcc gcgccgaagg tttacgaacc cgtccccgca
      721 ctgagtctca aacaggtggg cccgtgtatc cgtatcacgg gcccactcgc caggcctgca
      781 ccgggcgtga gctctcggcc cacactcgtt ctgctacatc gccggagctt tgacgcacac
      841 ccaaggctcg ccgggcacga agtcaccaac gttgaggcac atgtagcccg tgggacatag
      901 cgccgggtcc gcgtcacagc cggagacgga acagtatgcc ggctccgtgg gagagtaagc
      961 acaataattg gttgctccga aacagtccgt cttggagttg catagcgagc ccatttcgcc
     1021 gttgccattc gtggcgggcg gtggcttcac gcaaacccag ggctcacccg ggacgaagtc
     1081 gcccacattg aagcagctcc aatccggggg gcagatgctc ggatctgcgt cgcaccccga
     1141 gatcgagcaa taggcaggtt cgaaggcaga gaaggcacag taattcgtgg ttcccgcgca
     1201 gtcggtgtgc atcgtgcaca gctccgcgaa ggctacatcg gtggtatccg gtggttcgct
     1261 ggtagctcca ccgtccgatt gagaagtgcc gcctgcgtcc gcgccgccgg ggttgctcgc
     1321 cacggtgtcc ccgccgtttg cattcttgcc gccgctcgag gctttgccac cggttgccgc
     1381 gccacccgcg ttcgctggag tcttgccacc actcgcggtc gcggttccgc cagcatcgct
     1441 gccgtcaccc gccgcgttgc tgccaccgtt ccccgtgctg cctccattgc tcgagggcgg
     1501 ctcgggagcg ccgtactcga cgcacagatc gccctgacgg atgaagccag ttttgcaggt
     1561 gtcagggccg caaccgaaga agggcaagta aaggaccgcg ctggcgagca atatcgtctt
     1621 tcgcatactc attccttttg agttcaaaac gcgctcgccc ggactcgttt gtccctccag
     1681 ttccgctgag gaaggacggt tagaacacga ggcggcgcgg cacacgcctc ctgccaacgg
     1741 ttcaccggcg cccaggcgta agagttccgt tccgcgccag gagcgccaat tcgggcgcca
     1801 cgctctcgta tcttactcac gtgtcagctg tgctctgacg ctcgacacct gctcacgact
     1861 tcggacggca attccatgcg cctgtgccag cggtgccagg gaaaagccga ccattacctt
     1921 ccctgatggc ggaggctctt ggcggggagc agcgaccgaa cacgctcgat cgtgtccgcc
     1981 tgctcggccg ttttgtcgtc tcgatagcgc ttcaagcgtg cgaatcggag caccacgccg
     2041 caggcatact gcgcactcac ctgcacgtcg ttgaacgcaa tctctacgac gagctcgggg
     2101 cgcacgtgca ccacgtgtcc ttcgcgccca atctcgcgcg cgagcagcgc ctgggtttgc
     2161 atggtcaagg tcgcgtcggt catccccttg aaggtcttgc cgatcatggc gaaggccccc
     2221 gtatcggggt ctcgggcgcc gagatgcagg ttgctgagat accccgtgcg ccgtccactc
     2281 ccccactcgg cagcaaggac gacgagatcc agtgtatgca ccggcttgag cttgagccac
     2341 tccgagccgc gcctcccggc cgcgtacggt gcgctcagac ttttagccat tacgccctcg
     2401 taaccttcag ccagcgcccg cgagacgaaa gcatctgcct cgtctgcctg cttcgtgatg
     2461 agtcgtgaaa cgcggcagtc ctctggagag agagactgca gcgcctgtgc ccgctcctgc
     2521 cacgacgcgt cgatcagagt ctggtcatcg acgcgcagga gatcgaagaa gaacacgtcg
     2581 agcggcaatt cggcgcgcat ggcctccacg tccaacttcc gaccaaagcg acgcatggtt
     2641 acctggaatg gcaaaggcct gcgttctgac gagagcgcga tcgcctcgcc atccaacacc
     2701 agcttacgag ccggcaatgc agacactcgc tccacgatct cgggcaccgc cgccgtcacg
     2761 cggttcaggc tgcgactgta gacctccaca cgatcaccgt ccttgtggat ttgaacgcgc
     2821 gccccatcca ctttgtattc gaacgcggcc tcacccaagt tttggagcgc atcctccaca
     2881 tccgcagcgc tatcggccag catcggctgg atcgggcgaa acagctcgag ctcgaaggcc
     2941 gccagcgcgc tcagcccatc tcggcccagc gtctcaccaa cctttgcgag tgaacccgtc
     3001 aacatgaccg cgcgccgcac gcgatcggta ggtgccgaga acgcactcgc gaccgcgtcg
     3061 atcaccaccc cttctaaggc gccctggtgc agctcccctg aaatcaatcg gatcaggaac
     3121 tccgcttcgg gacgggtggc ccgtgcaaaa aggtccgcca atgccacggc tcgccgggag
     3181 ttcgagcccg accccttctc cgcttg
//
LOCUS       DASTKU010000238         3885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_715879, whole genome shotgun sequence.
ACCESSION   DASTKU010000238 DASTKU010000000
VERSION     DASTKU010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715879"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFQ61_08750"
     CDS             complement(<1..496)
                     /locus_tag="VFQ61_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954581.1"
                     /translation="MNLRAQIEALENLALLDASLAKREAELQSEGEVLSERKAQVQKL
                     EERYASTRASVGDMERTRGELLQDARQMSLQMERSREKLARCRTERELNATQREIEEL
                     RKLYRDRELEIEKLNGLADQARSEMDGANSEREALLGDLANESQVATRLGLLEKDLSA
                     EREQR"
     gene            complement(844..917)
                     /locus_tag="VFQ61_08755"
     tRNA            complement(844..917)
                     /locus_tag="VFQ61_08755"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(881..883),aa:Ile,seq:cat)
     gene            1276..>3885
                     /gene="uvrA"
                     /locus_tag="VFQ61_08760"
     CDS             1276..>3885
                     /gene="uvrA"
                     /locus_tag="VFQ61_08760"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9954582.1"
                     /translation="MQHLIVRGAKQHNLHDVDVELPRDRLVVITGPSGSGKSSLAFDT
                     IYAEGQRRYVESLSAYARQFLDQLPKPDVESIDGLSPAIAIEQKALGKSPRSTVGTVT
                     EIADYLRLLFSRVGVPHCPKSGKVLRAYTVQEIVDQILGLDEGSRLSLLAPVERNQEG
                     GLAERIERLRRDGFVRARVNGQAIDLAEPADWPEATRYDLDVVVDRIVVRAGTKGRVT
                     DSVELALKLGSGTLLLDAMDGSPPRVLSERLVSWEYGITLPPLEPRLFSFNSPHGACP
                     ACGGLGVVSRVDPERIVPDQQKTLREGAVVAFGRRGSTKMAAEVQAAVVGIGVDPDVA
                     WSALPAEQRQLILYGGSVKRARKKSTEYVGIVPRLERRLERDGAEAEDVEEEGDEYLD
                     PADEEDLGRFVVTRTCEACGGQRLRPEALAVKVFGSTIGQMCGLPLGELKQQLESFQT
                     SEAMNETEEIVAQPLIRAIVSRLGFLSDVGLDYLTLDRPAQTLSGGEGQRIRLATQIG
                     AALVGVLYVLDEPSVGLHARDNQRLLEALRKLVDKGNSVLMVEHDRDAILAADYLVDM
                     GPAAGKRGGRIVAQGTPAEVMANPESVTGPYLTGERNLPMPPRRQHPTGRALTIRGAR
                     AHNLRNVTVDIPLGLITAVTGVSGAGKSGLIVDTLLAATRARLYGASGWVGPYDGIDG
                     LDAIDKVVSIDQAPIGRTPRSNPATYTGVFTQLRELFATLPEARARGYKPGRFSFNVK
                     GGRCEACQGDGVLRVEMHFLPDVYVRCNTCGGKRYNRETLEIRYRGLSIADALELTVD
                     DAAQIFDAIPRIRQRLDALRSIGLGYLQLGQSATTLSGGEAQRVKLAAELARKSTGST
                     LYVMDEPTTGLHFS"
BASE COUNT          677 a         1288 c         1233 g          687 t
ORIGIN      
        1 tgcgttgctc gcgctccgcc gacaggtctt tttccaagag cccgaggcgc gttgccacct
       61 ggctctcatt ggccaaatcc ccgagcaacg cctcgcgctc cgagttggct ccgtccatct
      121 cgctccgcgc ctggtccgcc agaccgttca gcttctcgat ctccaactca cgatctcggt
      181 agagcttgcg tagctcttcg atttcgcgct gtgtcgcgtt caactcgcgc tcggtacggc
      241 agcgcgcgag cttctcgcgc gaacgctcca tctgcaggct catctgccgg gcatcttgca
      301 ggagctcgcc acgcgtccgt tccatgtctc cgacgctggc acgcgtactc gcataccgct
      361 cttcgagctt ttggacttgc gctttccgct ccgacagcac ctccccctcg ctctgcaact
      421 ccgcctcccg cttcgcaaga gaagcgtcca gcagcgccag gttctctagt gcttcaatct
      481 gcgctcttag attcacaggt acgttggctc ctcgttgcca gtttgccctt gggcgatacg
      541 gatacgctcc agccgccgca cgccgcagcg gatcggtgag cggttgggct ggcgagcagc
      601 ctgccccaac gcgcgaagtg ctcgcgcacg ctgtcctcgc gcacgctgtc ctcgcgcacg
      661 ctgtcctcgc gaaccatgcg ctcgcagaca ccgcgctcgc ctactttgcg ctcgtgtcag
      721 ccgctctccg agacaccact cttcgaaata ctgctcttcg aaacacggct cttccagaca
      781 cggctcttcg aaacacagct cttcgaaaca cagctcttcg aaacacagct cttcgaaaca
      841 cagtgggccc acctgggttc gaaccaggaa cagcccgggt atgagccggg ggctctgacc
      901 gattgagcta taggcccgga gaactaaaca tccctcgcga cacgcaacgc ctgcacggcc
      961 tcgggcgccc acgagcaacg accgagcatg caaggtcggc gcgccctcga gtagggaacg
     1021 gagccgctgg tttttgaaac tcttccacgt gctttcgcac ctcagctcca cggcccgaac
     1081 gttagtccac tctcggctcc gcggcaacgg ttcggggcgg attattcggc taccgcaccc
     1141 ctcggcccgt agccctagcg gtatactccg ggggtttcag cggcgcaggg gcgagtccag
     1201 aacgactttc gagccagctc gggctccgcc gcttgtccgg gacagtgacg gagttgtgct
     1261 acacgagcaa gctgcatgca gcatctgatc gttcgggggg cgaagcagca caatctccac
     1321 gacgtcgatg tggagctgcc ccgcgatcgc ctagtggtga tcacaggccc cagcggctcg
     1381 ggcaagagct cgctggcctt cgataccatc tacgcggaag ggcagcgccg gtacgtcgag
     1441 tccctgtccg cctacgctcg ccagttcctc gatcagctgc ccaaaccaga cgtcgagagc
     1501 atcgacgggc tgagtccggc cattgccatc gagcaaaaag cccttggcaa gagcccgcgt
     1561 tcaaccgtcg gaacggtgac ggaaatcgcc gactacctgc ggctcctgtt ttccagggtc
     1621 ggagttcctc actgcccgaa atcgggaaag gtgctgcgcg cctacacggt ccaggagatc
     1681 gtcgaccaga tcttgggcct cgacgagggc agccggctgt cgttgctcgc cccggtcgag
     1741 cgcaaccagg agggcgggct cgctgaacgg atcgagcgct tgcgccgcga cggcttcgta
     1801 cgcgctcgcg tcaacgggca agcgatcgac ctggcagagc ctgccgactg gcccgaagca
     1861 acgcgttacg acctcgacgt ggtcgtcgat cgcatcgtgg tgcgtgcggg gacgaaaggc
     1921 cgggtcacgg attcggtcga actcgccttg aagctcggca gcggtaccct gctcttggat
     1981 gcgatggacg gctccccgcc tcgcgtgctg agcgagcgcc tcgtcagctg ggaatacggc
     2041 atcacgctgc cgccgctcga gccccgactg ttttccttca atagtcccca cggtgcctgc
     2101 ccggcttgcg gcggcctcgg cgtcgtctcg cgggtagatc ccgagcgcat cgtccccgat
     2161 cagcagaaga ccctgcgaga gggggccgtc gtggccttcg gccggcgtgg ctcgacaaaa
     2221 atggcggccg aagttcaagc cgccgtcgtc gggattggcg tggatcccga cgtggcctgg
     2281 tcggctttac cagccgaaca gcgccagttg atcctgtacg ggggcagcgt caagcgagcg
     2341 cgaaagaaga gcaccgagta cgtcggcatc gtgccgcgcc tggaacgccg attggagcga
     2401 gacggcgcgg aggcggaaga tgtcgaggag gaaggcgacg aatatctcga tccggcggac
     2461 gaagaggatc tcggacgttt cgtcgtcacg cgcacgtgcg aagcctgcgg cgggcagcgc
     2521 ttgagacctg aggcgctcgc cgtcaaagtc tttggttcca cgatcggcca gatgtgcggg
     2581 ctgcccctcg gcgagctgaa acaacagctg gagagcttcc agacctccga ggccatgaac
     2641 gagaccgagg agatcgtggc ccagccgctc atccgtgcca tcgtgagccg gctcggcttt
     2701 ctgagcgacg ttggcttgga ctatctgacg ctcgatcgtc ccgcacagac gctgtcgggt
     2761 ggcgaggggc agcggatccg cctcgccact cagatcggag ccgcgctggt gggcgtcttg
     2821 tacgtgctcg acgagccgag cgtggggcta cacgcgcgtg acaatcaacg gctgctcgag
     2881 gccctgcgca agctggtgga caagggcaac agcgtgctca tggtcgagca tgatcgcgac
     2941 gcgattttgg cggcagacta tctggtcgac atggggccgg cggcgggtaa gcggggcggc
     3001 cgcatcgtgg cgcaggggac ccccgcggag gtgatggcga atccggaatc ggtgaccggt
     3061 ccctacctca cgggtgaacg caacctcccg atgccaccgc gccgccagca ccccacgggg
     3121 cgggcgctga ccatccgcgg cgcgcgcgcc cacaatcttc gaaacgtgac cgtggacatc
     3181 ccgttgggct tgatcacggc ggtcaccggt gtgagcggcg cgggcaagag cgggctgatc
     3241 gtggacacgc tgctggcggc cacgcgggca cgcctctacg gcgcatcggg ttgggtcggg
     3301 ccgtatgacg gaatcgatgg gctcgatgcc atcgacaaag tcgtcagcat cgatcaggcg
     3361 ccgatcggac gcacgccacg ttcgaatccc gcgacttaca ccggcgtgtt tacgcagcta
     3421 cgagagctgt tcgccaccct gcccgaggca cgcgcgcggg gctacaaacc aggccgcttc
     3481 tcgttcaatg tcaaaggtgg gcgctgcgaa gcctgccaag gcgacggcgt gctgcgcgtc
     3541 gagatgcatt ttctgccgga cgtgtatgtg cgctgcaaca cctgcggcgg aaagcgctac
     3601 aaccgagaga cgctcgagat ccgctaccgc ggcctatcca tcgcggatgc gctggaactg
     3661 acggtggacg acgcggctca gatcttcgat gccattcctc gcattcggca gcgtttagac
     3721 gcgctgcgct cgatcggact cggatatcta cagctcggac aatcggcaac gacgctgtcc
     3781 gggggcgaag ctcagcgggt caagctcgcg gcggagctcg ctcgcaagtc gacgggcagc
     3841 acgctctacg tgatggacga acccacgacc gggctgcatt tctcg
//
LOCUS       DASTKU010000239         8855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1832463, whole genome shotgun sequence.
ACCESSION   DASTKU010000239 DASTKU010000000
VERSION     DASTKU010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832463"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1457
                     /locus_tag="VFQ61_08765"
     CDS             24..1457
                     /locus_tag="VFQ61_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9954583.1"
                     /translation="MAKKKLAIIGNGMGTCRLLDELVQRRGHELYEITVYGEEPGGAY
                     NRISLGKVLAGEAPDAIVTKTAEWYDQHQIRLLSSTRVKRLDFKRKQIETEDEQRRSY
                     DLAVLATGSQPLVPPLEGMLGEDGELRPGAFVYRTIADCIRMRDYARPGDSAVVVGGG
                     LLGLEAAKVLSDQGLHVTVIHVANHLMNAQLDQIAGEMLAREIERTGIFVRTGRTVEA
                     IYGEERISGVILDDGTTLPADLVVLACGVRPRVDVARASGLPVNKGIIVNDTLATQVP
                     SVYAFGECAEHQGKIYGIVAPVWEQAVVLADVLTGSKPQSRYRGSKLYTRLKVAGVDV
                     ASMGRLEPELESDQVIQIIEERRNAYRKLVLRGDRLIGAMLVGNTAATAALVQIFDRD
                     DPMPEDPLEVLCPIRSAGPAPERLVCNCHKVTERAVCAAIEAGAESLTEIGEVTKAGT
                     GCGSCKTELAQLVSNLRKPAPAKVAAG"
     gene            1466..1897
                     /locus_tag="VFQ61_08770"
     CDS             1466..1897
                     /locus_tag="VFQ61_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9954584.1"
                     /translation="MSRLSASHYRQLAAFRYEIRKFLAFSERAAREAGVEPQQHQLLL
                     AVRGLPEGARPTIGTVAERLSVEPHTAVALVDKLEQRGLLRRERSTEDRREVLLKLTP
                     EGETTLKALSALHRQQLRSVGPSMVSALRAILDDHDEATGN"
     gene            complement(1953..2849)
                     /gene="modA"
                     /locus_tag="VFQ61_08775"
     CDS             complement(1953..2849)
                     /gene="modA"
                     /locus_tag="VFQ61_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9954585.1"
                     /translation="MAPLLSTTIRGSLVASLLVSSFCLTSAAIAAEVRVAVAANFATP
                     LKVLASEFSKRTGHRVIPSSGATGKLYAQIKNGAPFEVLLAADDKVPAQLEAEGLTVP
                     GSRFTYANGKLVLFSAKPGLVDAQGAVLRTGQFTHLAIANPKLAPYGAAAITVLTRLQ
                     LLQATQSKFVQGENIAQTMEFVVTGNAELGFVAWSQVLSDGKPRPGSYWMIPENLYPE
                     IRQEAVILKPGAENPAARAWCEFLKGEPARATIAVLGYGLPRAAAEVAAASGNGAGSE
                     PSPVKPSPAVKPTGTRSEKPVK"
     gene            3677..4426
                     /locus_tag="VFQ61_08780"
     CDS             3677..4426
                     /locus_tag="VFQ61_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954586.1"
                     /translation="MNRMRLFGVAYSTILSVFAFACGAQPGELEATNDDTSALTTAYC
                     KKAEPKDILIAQMVKDSLAANYPLTRLEVGQNGTVQGPDLPDVMVGDLGLINSIPEAR
                     TAVARAVSEVSGLPDYVVGSISNEQAACSSVPAWTPTGQTTASTTAISVTTTENEDSW
                     HETHKAFAKVSALLKRFGNKDIVDPPGDGSTNLPPSGSVSSTGVVANAYGFCPAGTSA
                     GTFCKLSYATGVNYMGRKCQYYYGYLRCLLY"
     gene            complement(4707..6443)
                     /locus_tag="VFQ61_08785"
     CDS             complement(4707..6443)
                     /locus_tag="VFQ61_08785"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9954587.1"
                     /translation="MSTSLLTDSSFLVEIARSPRIRPPLRIGTGELLDGRYELDVKLG
                     EGSFGVVYRARDHKLGRTVAVKVIRREGEGDANAVRELLAARIEPAPPNVVTLFDAGV
                     HDGYPFLVFEYLEGQTLQERIQLGRLSVRESLFVACQVARGLRAAHEAGVLHRDLKPS
                     NIFLTNAGDVKLLDFGLAHRLEDQQLVLARGGTPGFAPPEQSSGQPGEPRSDVFSAAM
                     TLYVCLYGGLPLNTLTFDFKMFRTEPPPAATILALTAPSAVKKLLIRATARELDKRPS
                     TAEWQERLERVLRQEMAPPPPLAKIAVGVFAGVCALGGMAAVLSGQSSDAIPKVRASS
                     PPSGSPLDAAAIGASPPAPARSTETLAMQLAAARFASWQSLDVRVQATAEARGRASQQ
                     PTRSRAIAPDASRRAAREVSGTSAREAAAPPMQGVPPPSIGEEEGWTLPAPPALSQLA
                     LRADPAELIKSNSISQNSESAPVPTAQLVGAPVAMDRPVAAPAGDPAGSPSTSGVPST
                     RGPTSAKPPSSVARSAAAPPLTPKAMARALQLKRRAQGERFLQGQSEPDRVRHRIVGF
                     FDSAPFNGARLR"
     gene            6968..7573
                     /locus_tag="VFQ61_08790"
     CDS             6968..7573
                     /locus_tag="VFQ61_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954588.1"
                     /translation="MNTLLRSLALGLLTLIPACNTSPEDSRSDYSDRGELRQAQIVAE
                     CRNTTKDCLKGAVTVTDIRACREVFTACAGDAIDLVDSGLAEAIDLAEHAVDITVDQA
                     TGLLGDVGGLTSHALNAVDECRAGVTQCLSGVVSVSDISKCQEVFDACVGGAMSVAGV
                     GIQIDLPGSSKTGCSVGVSACLGSGKPPAQCAIGAQLCLSH"
     gene            7587..8186
                     /locus_tag="VFQ61_08795"
     CDS             7587..8186
                     /locus_tag="VFQ61_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954589.1"
                     /translation="MKTKVSWRLLTGILATSSCWITACGGGSDEPSPAGAAGTTSNST
                     GGAGGTTALSGGTSASIGSAGSTGANTAGRTHVDATGGRQSDIEGGHPNAGAAGDGAA
                     QVGGSDSHSAGSGGEDGGTPPTSAGAGGAGAGGADDANQGPSSCCLVHTASGCGDTSV
                     ESCVCAKDPRCCSGNWDAFCVLLAAAPTANGACTVCSGL"
     gene            8270..>8855
                     /locus_tag="VFQ61_08800"
     CDS             8270..>8855
                     /locus_tag="VFQ61_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954590.1"
                     /translation="MNLYFVRAWTRIGTSLLLTLCACAQPNAAETRVRTDTPASTAVE
                     PGGGGGSYVGGSTSSQTPQLPNASGGAPILGQSGASGAITSAAGGATGTATQTSPTCG
                     SVQIGSELKTTETVIEVPGTVLFVFDQSSSMNEDWNGTPKWSVANAALVAAFAPLQGK
                     LSAGAVLFPTPSADTSTDRCNWLVDWRSCVAAGSS"
BASE COUNT         1652 a         2781 c         2814 g         1608 t
ORIGIN      
        1 ctgagcggcg agacctcaag taaatggcga aaaagaagct ggctatcatt ggtaacggaa
       61 tgggcacctg ccggttgctg gacgaactcg tgcagcgccg cggccacgag ctctacgaaa
      121 tcaccgtcta cggtgaggag cccggcgggg cctacaatcg gatctcgttg ggcaaggtgc
      181 tcgcaggaga ggcgcccgac gcgatcgtga ccaagacggc cgaatggtac gatcagcatc
      241 agattcgcct gctctcgagc acacgcgtga agcggctcga cttcaagcgc aagcagatcg
      301 aaaccgagga cgagcagcgc cgcagctacg atctagccgt attggctacc ggcagtcagc
      361 ccctggttcc accactcgag ggcatgctcg gcgaagatgg tgaattgcgc ccgggggcgt
      421 tcgtgtaccg caccatcgcc gattgcatac gcatgcgtga ctatgcacgc cccggcgaca
      481 gcgcggtggt ggtcgggggc ggactactcg gcctcgaggc ggcgaaagtg ctctcggatc
      541 agggtctgca cgtcaccgta atccacgtcg ccaatcacct gatgaatgca cagttggatc
      601 aaatcgcggg cgagatgctg gcgcgggaga tcgagcggac gggcatcttc gtgcgaaccg
      661 gcagaaccgt ggaagcaatc tacggagagg agcgcatttc cggggtgatc ctcgatgacg
      721 gcaccacgct gcccgctgac ttggtggtgc tcgcgtgtgg ggtacggcct cgagtcgacg
      781 tagcgcgcgc atctgggcta ccggtcaata aggggatcat cgtcaacgac actctggcca
      841 cccaagtgcc gtctgtctac gccttcgggg agtgcgcgga gcatcaaggc aagatctacg
      901 gaatcgtcgc cccggtgtgg gaacaagcgg tggtgctcgc ggatgtactc acgggttcga
      961 agccgcagtc tcgctaccgt ggatcgaagc tgtatacccg cctgaaggtg gccggtgtcg
     1021 atgtcgcttc gatggggcgg ctcgaacccg agctcgagag cgaccaagtg attcaaatca
     1081 tcgaagagcg gcgcaatgcc tatcgcaagt tggtattgcg cggagaccgc ctgattggtg
     1141 ccatgttggt cggcaacacc gccgcgacgg ccgccctcgt gcagatcttc gatcgggacg
     1201 atcccatgcc cgaagatccg ctcgaggtgt tgtgcccgat tcggagcgcg gggccggctc
     1261 cggaacgctt ggtgtgcaat tgtcacaagg tgaccgagcg cgcggtttgc gcagccatcg
     1321 aagcaggagc cgagtcgttg acggaaatcg gggaagtcac caaagctggt acgggttgcg
     1381 gctcatgtaa gaccgagctg gctcagctgg ttagcaatct acgcaaaccc gctcccgcca
     1441 aggtagccgc cgggtaagtc gaggcatgtc gagactttcc gcgtcgcact accgtcagct
     1501 ggccgccttc cgctacgaaa tccggaagtt cctggctttc agcgagcgtg cggcgcgcga
     1561 agccggagtg gagccacagc aacatcaact cctcttggcc gtgcgcgggc tgcccgaagg
     1621 cgcacggccg accattggca cggtggcgga gcgtctgtcg gtggagcccc acacagcggt
     1681 ggctttggtc gataaactgg agcaacgggg cctgctacgt cgcgagcgaa gcaccgagga
     1741 tcgccgcgaa gtattgctca agctcactcc ggaaggtgag accactttga aggcgctatc
     1801 cgcgctgcac cggcagcagc tccgtagcgt cgggccaagc atggtcagcg cgcttcgcgc
     1861 cattctcgac gaccatgacg aggctacggg gaactgagga gctctcagcc agaattcgcg
     1921 cctcctcgaa tccgcgcgaa gtatgggccc aatcacttca ccggcttctc gctgcgagta
     1981 ccagtgggtt tgacggcggg gctgggtttg accggactgg gttcactccc agccccattg
     2041 cccgaggccg cagcaacctc ggctgccgct cgcggcaaac cgtaacctag gaccgcgatc
     2101 gtggctcgtg cgggctcgcc cttcaggaac tcgcaccagg cgcgcgcagc gggattctcg
     2161 gccccgggct tgaggatcac cgcttcctgc cggatttctg gatacaggtt ttcgggtatc
     2221 atccagtagg agccgggtcg tggtttccca tcactcagca cttgcgacca agccacgaaa
     2281 ccgagctcgg cgttgcccgt aacgacgaac tccatggttt gcgcgatgtt ctccccttgc
     2341 acgaacttcg actgcgtcgc ttgcaatagc tgcagtcgag tgagtaccgt aatcgcggcc
     2401 gccccataag gcgcaagttt gggatttgcg atcgccagat gggtgaactg gccagtccgg
     2461 agtacggccc cctgagcgtc taccaatccg ggcttcgcac tgaagagcac gagcttgccg
     2521 ttggcgtaag tgaaacgcga acccggcacc gtcagcccct ccgcctcgag ttgggcgggg
     2581 actttgtcgt cggcagccag cagaacctcg aatggcgcgc cgttcttgat ttgggcgtac
     2641 aacttgccgg ttgcgccact cgaggggatc acccggtgac ccgttctctt gctgaactcc
     2701 gaggcgagga ccttgagcgg ggtcgcgaag ttggcggcca cggcgactcg cacctccgcc
     2761 gcgatcgcgg cggaggtcag gcaaaatgaa ctcaccaaca gagaggcgac caatgaaccg
     2821 cggatcgtag tgctaagcag tggagccaca cgtctctcta gctcgaaatc cgcgcgcgtg
     2881 caggacgcga cgatgagctt tgtgcggcgt aatcgcctct tcaatgtttc cgctcggctg
     2941 cgtgccggct tcgggcctgt gtttctggcc tatgttccgg gcctacgctt cgcgcatgtg
     3001 acctgcgggc aaccaatcat tcggctattg ccgccttcgt cgcatgcgcg gtccgagcgt
     3061 ggcggtacga gtgacgatgt ttacgcgggc gcgcggaggt catcggccgt cctgtttagg
     3121 cgatgcaaac ccagggtctt tgccgattga gtctggccgt cgctccctcg ttgcaaccta
     3181 tgacgacgat tcttgggcac atggttcacc cacgttcact ccccaggggc gtgcccgcgt
     3241 cgcgaacccc ccgggttggc gaagtgcctc accgctgctg ctcagtacaa cgagtgtcac
     3301 ggcgccgacg aagcatcaat gtgtcaatcc agtttgcggc ttggccatac atcacctgtg
     3361 tgggatatct tcgcttgctt caccagagct agtcagactg gcgcacttcg ccatctatcg
     3421 accatacaat agcgctctgt gttatcgaaa gaatccggac acttgcacgc cccatcgttt
     3481 caatgaccat agtcttggag cgcagcgacg tgcgcttggt gatcagggtc gtaccactac
     3541 gaaggataaa ctcatctttc aaaatacacc cggagtgtgt gaattcatat actccacaac
     3601 tcacggcatc tggattgcaa ctctccggaa cgtgcgcagc tcgctatcgt gcgcacgaac
     3661 acaacaagga gatgttatga atcggatgcg actgttcgga gtagcgtatt ccactatctt
     3721 gagtgtgttt gcttttgcct gcggcgctca gcccggtgag ttagaagcca cgaacgatga
     3781 cacctcggcc ttgaccacag cctactgcaa gaaggcggag cccaaagaca tcttgattgc
     3841 ccaaatggtc aaggacagcc tggccgccaa ttatcctttg acacgcctgg aggttggaca
     3901 aaacggaaca gtccaggggc ccgatcttcc ggatgtgatg gttggtgatc taggcctcat
     3961 caacagcatt ccggaggcgc gcacggccgt ggcgcgcgcg gtaagcgaag tgtccggact
     4021 accagactac gtggttggga gcatctcgaa cgagcaagcg gcgtgcagct cggtaccagc
     4081 ctggaccccc acgggtcaga ccaccgcgtc taccacggcg attagcgtga ctacgaccga
     4141 gaacgaggac tcttggcacg agacccacaa ggcttttgcc aaagtgagcg cgctactcaa
     4201 gcgcttcgga aataaggata tcgtcgatcc acccggtgac gggagcacga acctaccccc
     4261 gagcggctcg gtcagctcca cgggcgtggt cgcgaatgcg tatgggttct gcccagccgg
     4321 cacttccgcg ggcaccttct gcaaattgag ctacgcgacg ggcgtcaatt acatgggtcg
     4381 caagtgccag tactactacg gctatctgcg ttgcttgctg tactgagcgc ctgcccgcaa
     4441 cttcgagtcg tgcggcggct cacgagtcgg tgacgggagc agatatcgag taagaagcac
     4501 catgacggcg gcgccctgcc atcagcccgg gcgccgcggt tggcctaggg ccagagcctt
     4561 tcctgggacc acgctcgcag atcctggctt gtgctcagcg aggcacgcgg cggacgccgg
     4621 gatctacgag cggtccgttt gaggtcgctg tcctttgact ctgcgccttc gacggcgtgg
     4681 cttgaacgag gaccgcgttc ccaacgtcag cgcaatcggg caccgttgaa gggggcactg
     4741 tcgaagaagc ccacgattcg atgccggacg cgatcgggtt cactctggcc ttgaaggaat
     4801 cgctcgccct gagctcggcg cttcagctgc agcgctcggg ccatggcctt gggcgtgagt
     4861 gggggagcgg cagcgctccg agccacggag gagggtggct tggccgaggt tggtccacga
     4921 gtggagggca ctccgctcgt ggacggactg ccagctggat cgcccgccgg agccgcgacc
     4981 ggccgatcca ttgcgaccgg agcgcccacg agctgcgccg taggcactgg cgcagactct
     5041 gagttttgag atatcgagtt tgattttatc aactccgcgg gatctgcgcg cagcgccagc
     5101 tgggacaagg ctggaggtgc gggtagcgtc caaccttcct cctcgccaat cgagggaggc
     5161 gggacacctt gcatcggcgg ggctgccgcc tcacgcgcgc tcgtcccgga gacttcacga
     5221 gcagcgcgac ggctagcgtc cggggcgatc gcgcgtgagc gtgtgggctg ctgggacgca
     5281 cgcccccgcg cctcggccgt agcctgcact cgaacatcca aggattgcca gctcgcaaag
     5341 cgcgcagcgg caagttgcat ggccaaggtc tcggttgagc gtgccggcgc cggtgggctg
     5401 gcccctatag cggctgcatc aagtgggctg ccgctaggtg gggagctagc gcggaccttc
     5461 gggatggcgt cagagctttg gccggagagc acggcggcca tgccgcccag cgcgcatacc
     5521 ccggcgaaca caccgacggc aatcttcgcg aggggcggtg gcggtgccat ttcctgtcgt
     5581 aatacgcgct cgaggcgctc ctgccactcc gccgtgctcg gtcgcttgtc gagttcacgc
     5641 gcggtcgctc gaatcaacag cttcttcacc gcactcggtg ccgtcagagc cagaatcgta
     5701 gccgcaggcg gcggctcggt cctgaacatc ttgaagtcga aggtcagcgt attgagcgga
     5761 agcccaccgt aaaggcatac atagagtgtc atcgccgcac tgaacacatc actccgcggc
     5821 tcgccaggtt gcccggagga ttgttcgggc ggcgcgaagc ccggcgttcc gccccgcgcg
     5881 agcacgagct gttggtcttc caggcgatgg gcaagcccga agtccaacag cttcacgtcc
     5941 cccgcgttgg tcagaaagat gttgctgggc ttcaggtctc ggtgcagcac ccccgcctca
     6001 tgagcagcgc gtaacccgcg cgcgacctgg caggcgacga acagggactc gcgcaccgat
     6061 agccggccga gctgtatgcg ctcctgcaac gtttgccctt ccagatactc gaagactaga
     6121 aaagggtaac cgtcgtgcac gccggcatcg aacaaggtga ccacgttggg cggcgcaggt
     6181 tcgatgcggg cagccagtag ttcccgcacg gcattcgcgt ccccctcgcc ttcacgacgg
     6241 atcaccttga ccgccacggt tctgcccagc ttgtgatcgc gcgcccggta cacgactccg
     6301 aaggacccct caccgagttt cacgtccagc tcgtagcgcc cgtccagcaa ctcccccgtc
     6361 ccaatccgga gcggtggacg aatgcgggga gagcgagcga tctccaccag gaacgacgaa
     6421 tcagtgagga gtgacgtact cacggcgacc tctgtgaacg caattggctc cgtgggcgcg
     6481 cgctcgattg gtatgtatcg ctcgcgccgc aagctaggct cttcgattgt cgaggctgac
     6541 tatcgcccag ttttgcgtcc tggatttcca tcgaacgaat tcccagctct cgtgttccct
     6601 tcttcctcga tagcacggag ccgctccgcg gtgccagcgc tcgagagcgc acccattcga
     6661 atcccgccca cccgaaacca tgcgcgcgcg ccacgggccg tcgggcaagg cccgctcgtc
     6721 cacggatcgc cctcccacac gggctaaggc cacccgtttg ctgctgacaa ggatacgcgc
     6781 ggtcggcgag acgctgcgaa gccttcgagc gctgcaaagc gcaacacgcc ttctcgtttt
     6841 gaacagccca cgccaacggt actcagtgcc ggcgacgctc ataaaatctg cccccgactc
     6901 tgcagtcacg cagcacttag aggctgtcac aacatcgacg ccgagtacga ggcgtcggga
     6961 gagatccatg aacacgcttc tacgttcact cgcattgggc cttctgactt tgattcccgc
     7021 gtgcaacacc agcccggaag actcgcgctc ggactactcc gatcgaggag agctgaggca
     7081 ggcacagatc gtcgccgaat gccgcaacac caccaaagat tgcttgaagg gcgcggtcac
     7141 cgttaccgat attcgcgcgt gtcgagaggt cttcacggca tgcgcgggag atgcaatcga
     7201 tctcgtcgac agcggcttgg ccgaagcgat cgatttggcg gagcacgccg tcgacatcac
     7261 cgtcgatcaa gccacaggct tactgggaga cgttggcggg ctgacctcac acgccttgaa
     7321 cgcggtcgac gaatgccgtg ccggcgtgac ccagtgcctg agcggtgtgg tctcggtcag
     7381 tgacatttcg aagtgtcagg aagtcttcga cgcctgcgtc ggcggcgcca tgtctgtcgc
     7441 gggcgtcggg atccaaatcg atctgcccgg ctcttcgaag acgggctgca gcgtcggtgt
     7501 tagcgcttgc ctcggcagtg gcaaaccgcc ggcccaatgc gccatcggcg cccagctttg
     7561 tctctcccac tgattgggca tcaaccatga agaccaaagt ctcgtggcgc ctgctcaccg
     7621 gaatcctcgc gacgtcctct tgctggatca ccgcctgtgg cggaggctca gacgaaccgt
     7681 cacccgccgg cgcagcgggg accacgtcca actcgaccgg cggtgccggc gggacgaccg
     7741 cgcttagcgg cggcaccagt gcctcgattg gctccgcggg atctacggga gccaacacgg
     7801 caggtcgcac ccacgtcgac gcaaccggag gcaggcagtc cgatatcgag ggcggccacc
     7861 cgaatgcagg tgccgcgggc gatggtgctg cgcaagtcgg cggtagtgat tcccactctg
     7921 ccggcagcgg cggggaagac ggcggaacgc cgccgacaag cgctggcgcc ggcggagctg
     7981 gggccggcgg cgccgatgac gcgaaccaag gcccgagttc gtgctgcctt gtccacacgg
     8041 cttctggttg tggggacacg agcgtcgaaa gctgcgtgtg cgccaaggat ccacgttgct
     8101 gcagcggcaa ctgggacgct ttttgcgtct tgctagccgc ggcgcccacc gccaacggcg
     8161 cgtgcacggt gtgcagcggt ctttgaaccc gtttcgctgc aagacgtctg cgcgtcagcg
     8221 cgctctgaga atgaagatag gctgtcagtc ttctgcccgg gagcctccca tgaatctgta
     8281 tttcgtaaga gcttggactc gaataggcac ctcgctattg ctgacgttgt gcgcctgcgc
     8341 ccagccgaac gccgccgaga cgcgcgttcg gaccgacacg cccgcgtcca ccgccgtcga
     8401 gcccgggggc ggaggcggaa gttacgtcgg aggcagcacc tcgagccaga ctccgcagct
     8461 gccgaatgcg tccggggggg cgccgattct cgggcaatcg ggcgcgagcg gcgcgatcac
     8521 gtctgccgcg gggggcgcca cgggtacggc cacgcaaact tcgcccacct gcggcagcgt
     8581 gcagattggc tccgagctca agaccaccga aaccgtaatc gaggtcccgg gtacggtgct
     8641 gttcgtgttc gatcaatcgt cgagcatgaa cgaggactgg aacggcaccc caaagtggtc
     8701 cgtggcaaac gcggcgctgg tcgccgcgtt tgcaccgcta caaggcaagc tgagcgccgg
     8761 cgcggtcttg ttccccacac cttccgccga tacctccacg gatcgctgca attggttggt
     8821 ggattggcgc agctgcgtcg cagcaggctc atcgc
//
LOCUS       DASTKU010000240         6872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_939709, whole genome shotgun sequence.
ACCESSION   DASTKU010000240 DASTKU010000000
VERSION     DASTKU010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939709"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1519
                     /locus_tag="VFQ61_08805"
     CDS             191..1519
                     /locus_tag="VFQ61_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954591.1"
                     /translation="MRPTTTPMRESDPLRLDRTLRGYAKRYVDFRLALRTGPAQEHVF
                     ELLPRELSDEGLEELAESQAGDPLRSAALPIAVWLRIEHDLIEARREEAVRLRHTRFE
                     IDQPVRGSFSLAELRRSMLADPLRRSGFLSALLGRAERLGEARVRRWELEGELYARHS
                     TPAARLGSASERAVSESLLKGTDAAYAELGLQRFDAYLERALGRDVSAQFPRRLSLRT
                     LADYFTDSPLLRGLPLEAYAAPEMLGSASFVLGMAGFGERFFEAATHNEHAFVMRFDL
                     RAASRAEWAALFALLPASRPFASRYLDVPRSRYGEYSLRLGQTWVALLRGLTLRAALV
                     EPSLAGERSYLAAFRDLVPRALGFELDRRAAGALFVDDMAVTRLSGLALAAARARELT
                     EAYDEDWFRNPRAIDQIREELRLCEPATPSSEQLELGVRELLRWLHEQLG"
     gene            complement(2141..3082)
                     /locus_tag="VFQ61_08810"
     CDS             complement(2141..3082)
                     /locus_tag="VFQ61_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954592.1"
                     /translation="MSLGTLCCIREAAAERSDLDPTIGYNYGEVETAHTAGVAAAQRA
                     GSNSVSALFLNPANIAVGRVYHVTAFAHVLPEAQRQTYGAAATDSVSSPIHLAGAAGV
                     TYNLQDSSGIKRRYTDVRFALAFPFSDRFFVGLGGRYLMLRQNGDGPLGDSLASGGLS
                     GLNIVRAFSLDAGTTFKPTPEISLALVGTGLENAGHGFLPTTLGSGVAYGRREFSLEG
                     DLVFDFTTWQETRLRAQTGLDLLLADHYGVRAGYRFDQGAESHALSLGAGYIDRAFAF
                     DLALRRTVAGATSTSIMAGFVVHLDNVGLSPSPSETF"
     gene            4653..6080
                     /gene="dnaA"
                     /locus_tag="VFQ61_08815"
     CDS             4653..6080
                     /gene="dnaA"
                     /locus_tag="VFQ61_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET9954593.1"
                     /translation="MSIPPANERVEQSWKDAVEETRRRSPASFEQWFSSVQFDGLDEG
                     VLCLSARDEWVRDWVIAHFLPDLLANIERRLGDEARGGLRVKWQIQANIEQPVCERRV
                     VPARPVRPSEPPPSRRSERPSGISYARTTLAAALNPKHTFPNFVVGPSNALAYAAAFA
                     SAGGGGPRYNPLFIAGGTGLGKTHLMHAIAHKVLEERPSARVMYVSAERFTNDFIEAL
                     QHHRMDEFRARYRAECDLLLVDDIQFLAGREQTQEEFFHTFNALRDADRPIVVTSDKY
                     PQQLQRMPERLVSRFTSGLVADIQVPQLETRVAIVKKKAQLEGIDLTDDVAVLLAQNV
                     QSNIRELEGALIRLAAKSSLTGRPIDEEFARSELSVVGPRRPDLMSVEDIQRAVCSHF
                     RLSNAELLSKDRHKSVAFARQVAMYLCRQRLKCSFPELGRAFGNRDHTTVMSAVRRVE
                     ALRQRDPQVNAHLEAIEQRLASSEE"
     gene            complement(6150..6350)
                     /locus_tag="VFQ61_08820"
     CDS             complement(6150..6350)
                     /locus_tag="VFQ61_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954594.1"
                     /translation="MTTLRHIHRYMNDRRRVVLDDGRAGKIVRVDTVFPENETYVTVI
                     TDAASDSSVKVLLSQVKGPAES"
     gene            6489..>6872
                     /locus_tag="VFQ61_08825"
     CDS             6489..>6872
                     /locus_tag="VFQ61_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954595.1"
                     /translation="MSLRTQCSRRLGTLIPRWSTPTAAALLGLHLLCGCSLAHTATVR
                     TAALPPPSEPVIRHSARLWLDIFPGVNGTQCVMPPQATREVCFDAVEDAVQASLSRVL
                     WTSFPRVDVLGRGDTPAPEDYVLHLD"
BASE COUNT         1340 a         2190 c         2122 g         1220 t
ORIGIN      
        1 caaagccacg ccgatcgcga cccagtgtac ggcgggacgg gccatcgctt cggcgtcttc
       61 ggaggcgggt gtttgcacga caggtaagcg gcggaccaca gcaagactgt ggcgacgacc
      121 gcatgcaccc gcaagcgatg ggcctgaacg atcgacggta ccggggctcg gggctgccct
      181 tggtgctaag gtgcggccca cgacgacgcc catgcgagag tccgatcccc ttcgcctcga
      241 ccgaaccctc cgcggctacg ccaagcggta cgtcgacttc cggttggcgc ttcgcacggg
      301 ccccgcccag gagcacgtgt tcgagctgct cccgcgagag ctctccgatg aggggctaga
      361 agaactcgcg gagagccaag cgggggatcc gctgcgatcc gcggcgctgc cgatcgcggt
      421 ttggctgcgc atcgagcacg acttgatcga agcgcggcga gaagaagcag tgcgactccg
      481 ccacacccgt ttcgagatcg atcagccggt gcggggcagt ttcagcctcg cggagttgcg
      541 gcgctcgatg cttgctgatc cgctgcggcg ttcgggtttt ttgagcgcgc tattggggcg
      601 tgcggagcgc ctcggcgaag cccgcgtgcg ccgctgggaa ctagaaggcg agctgtacgc
      661 acggcattcg acaccagctg cgcgactggg atccgcgagc gagcgggccg tgagcgagtc
      721 gctcctgaag ggcactgacg ccgcgtacgc cgagctcggc ctccaacgct tcgacgcgta
      781 tctggagcgc gctctgggtc gagacgtatc cgcacagttt ccccgacgcc tctcgttgcg
      841 cacgctcgct gactacttca cggatagccc actgctgcga gggctgcccc tcgaagccta
      901 cgccgcgccg gagatgttgg gctcggcgag cttcgtgttg ggcatggccg gctttggaga
      961 gcgcttcttc gaagctgcga ctcacaacga gcacgcgttc gtgatgcgct tcgatctgcg
     1021 ggctgcctct cgagccgagt gggccgcgct gttcgcgctg ctgcccgcgt cgcgtccctt
     1081 cgcgagtcga tatttggacg ttcctcgctc gcggtacggt gagtactcac tgcgcttggg
     1141 gcagacctgg gttgctctgc tgcgtggcct aaccttgcgc gctgcactcg tggagccgag
     1201 tttggccggt gaacgcagct atttggctgc ctttcgcgat ctggtgccgc gcgcgctggg
     1261 cttcgagctg gatcgtcgtg cggcgggcgc gctgttcgtc gatgacatgg cagtgacgcg
     1321 cctgagcggc ctcgctttgg ccgccgcgcg agctcgcgaa ctgaccgaag cctatgacga
     1381 ggactggttt agaaacccta gagcaatcga ccaaatacgc gaagagctcc ggttatgcga
     1441 accggccacg ccgagctccg aacagctaga gctgggcgtg cgtgaattgc tgcgctggct
     1501 gcacgaacag ctcggttgag caccccacaa ctgctgggcg gtatctgtca gcggtacctg
     1561 tcagcggtgc acgttcgcgg tgcatgcggc tgagcagcgt gacgacttgg cccgacgcgc
     1621 gaaaatcgac gagagaagca caactcgcgc cctttgagtc agacctcatg taggatcgtc
     1681 cgcctaggga aggtcatgcg caacactctg ctggttgcat ggttggcgtt ggtgctcggc
     1741 tcgtgctcgt cgctgttcga tacgaaccac aaccccaagc cggccttcaa cgccgtggtc
     1801 gatttttgag gggaccccag atgatttcga caggaaccga gctgcgctcc tgggtgggcg
     1861 aatcaagaag gtggaagcta ctcgttagtc tggtttcgct gaccgcgttg gcgggcgtgt
     1921 tcattgggtg cacgcggggc ttgcccacgc gcacgccgga gtgcaacgcc atcatcaatc
     1981 gttgcatggc ccaatgctcg aacgcccccg acacactctc atcacgttcc atggcggcgg
     2041 acgacgggcg ccacgcggta cctcgctcga gcgggtgcga gcaatcttgc agcagcaaat
     2101 gccgctgaac tcgcgcgccc cgtgaaggac cgcgaacccc tcagaacgtc tcggacggag
     2161 aagggctcaa gcccacgttg tccagatgaa ccacgaagcc cgccatgatg gaggtcgagg
     2221 tcgcgcccgc tacagtacgc cgcagcgcca agtcgaaagc aaacgcccga tcgatgtaac
     2281 cggcgccgag cgagagcgcg tggctctccg ccccctgatc gaaacgataa ccagcgcgaa
     2341 ctccgtagtg gtcggcaagc aacagatcca acccggtttg cgctcgcagg cgcgtctctt
     2401 gccaggtcgt gaagtcgaac accaaatcgc cttccagaga aaactctctc ctaccgtagg
     2461 cgacgccact cccgagcgta gtgggcagga agccatggcc tgcgttctcg aggcccgtgc
     2521 ccaccagcgc gagcgagatc tccggagtcg gcttgaaggt ggtgcccgcg tcgagtgaga
     2581 acgcccgcac gatgttcagt cccgacaagc cccccgatgc caagctgtca ccgagcggcc
     2641 catcgccgtt ctgacgcagc atcaagtagc gcccgcccaa tccgacgaag aaccgatccg
     2701 agaacggaaa cgccaacgcg aaccgcacgt ccgtgtaacg tcgcttgatt cccgaagagt
     2761 cttgcaagtt gtaggtgacc cccgccgctc ccgccaaatg gatcggtgag ctcaccgagt
     2821 cggtggcggc agccccgtac gtttgtctct gggcctccgg cagaacgtgc gcgaaagcgg
     2881 tcacgtgata aacgcgcccc accgcaatgt tcgcgggatt aaggaacaga gcactcaccg
     2941 agttggagcc cgcgcgctgc gccgcagcca cacccgcggt gtgtgcggtc tcgacctccc
     3001 cgtaattgta accgatggtg ggatccaggt cgctccgctc cgccgctgct tctcgaatgc
     3061 agcacaacgt gccgagcgac atgccacagc tcagtttgca gagaacccac gcgtagcgcc
     3121 gcaagcgctt tgtcacactc ttcggtgcgg tccgtttcac cgacagagcg ctagcccagc
     3181 atgcctgcga aggccaacta ttcggtcagc atccgaagaa tctgcgcgga gcgacacgac
     3241 gccgacgtga acccgaacgt caagcactgc ttgtaggtcc gacgcagatc ggtgtctcaa
     3301 catccgagcg gccggaagcg cgtcatgcac ttgccagagt gcaccgatga gaagtgactg
     3361 cgcgtgctca tcacgcgacg tgagatcgtc gtgcgcgatc atgcgcgtgc acacgctctc
     3421 tgttccgaaa cacgtgcgtg cagatggtgc cgcaaacgac tgaacatgtg actccgaatc
     3481 atcatgaaac agcagaattg atgggctgca gaaagcgtca cacactcact ctccggagta
     3541 gtaggaacat gcacttgcaa agtggccgtt cggtcagcaa acaatgttct gcgtgcactc
     3601 ttgacgcaca aaccaaggat cttcccgctg cgtgcggagc acgcgcgcgt gcgcgcgatg
     3661 gaatccgatt ggactctgcg aagttactga gcgtgctccg agttgccgat cgacaagcgc
     3721 atgcgcatga agatttcatc gtccacgatc ccgcgtcatt tgcgcggctc atgagtgtcg
     3781 tgaatctttt cgttgaactt tgtcttccgc cgatgctagc tgcgcccctc ttcgattggt
     3841 tgcgggcgac gcaccgcttt tctagaccgc ccggccgccg aagatgacgt cggacggcgt
     3901 tggcgagggc aagccgagcg aaagcgaaaa ctggcccgga tcaagcgaaa aatgaggcaa
     3961 cgcaacttgg cgatgcaaag cacgaagcaa tgccccttca cgcattagaa tgcgcaacgc
     4021 aaacgaaagc tcgagacgaa aattagaacg cgcaacgcaa gctgaaaggt gcatggtgag
     4081 ggaggtgtca cctcgtgcca tgcgcatcag cgccccacgc gaaccgagtt aggcgacgca
     4141 aaacgacgcc ccgtgattca atccggtcgt agccaagaag agaacggcac gacgacagaa
     4201 cggcacggct cacgacagag acgacagaac agcgtagcca cgaacgaaca gcgcggccga
     4261 ggacggaaag ggagcggcgc aagcagacgc gattcgctcg aggcaacggt caccgaagcg
     4321 gtcaacgtcc aaccgagacc gctaaaccga aacagcacaa cagaatcgaa gagggtcgat
     4381 gagtaagtct ctcagggaac ttccgagtcg aactttctat tctcacaact ccgaatccca
     4441 cgatgctgga cattgcagct ggcctctgac agaaggcccc gctccagagc tcgctcacca
     4501 caagtgatgc tgcgagacag ccgaaggaca gaattatcgt ttcaccgtcg tacaccccaa
     4561 ctaatccaca gactgtggaa aagctgggga tttcccgctg cgagacgtcc gtgatcagca
     4621 atactttcga gctccatgct tcaggttccc ccgtgagcat tcctcccgcg aacgaacgcg
     4681 tcgaacagtc ttggaaagat gccgtcgaag agacgcgccg ccggtctcct gcttcattcg
     4741 agcagtggtt ctcgagtgtg caattcgacg ggctggacga gggcgtgttg tgtctatcgg
     4801 cccgcgacga atgggtacgt gattgggtga tcgcacactt cctcccggac ctgctcgcga
     4861 acatcgagcg gcgcttgggt gacgaagcgc gtggtgggct gcgtgtgaag tggcagatcc
     4921 aagccaacat cgagcagccg gtctgtgaac gccgcgtcgt cccagctcgc cccgtaaggc
     4981 cgagcgaacc cccgccgtca cgtcgcagcg agcgccccag tggcatcagc tacgcgcgga
     5041 ccacgctggc cgctgcgctc aaccccaagc acacgttccc caacttcgtc gtcggcccat
     5101 cgaacgcgct ggcctatgcc gcggccttcg cttcggccgg tggcgggggg cctcgctaca
     5161 accccttgtt catcgcgggt ggaaccggtc tcgggaaaac ccacttgatg cacgccatcg
     5221 cacacaaggt gctggaagag cgcccgagcg cgcgcgtgat gtacgtctcc gccgagcgct
     5281 tcaccaacga cttcatcgag gccttgcagc accatcgaat ggacgagttc cgcgctcgct
     5341 atcgcgcgga atgtgatctg ctgttggtag acgacatcca gttcttggcc gggcgcgagc
     5401 agacgcagga agagttcttc catacgttca acgctctgcg tgatgccgac cgtccgattg
     5461 tcgtgacgag cgacaagtat ccgcagcagc tacagcgcat gccagaacgg ttggtctcgc
     5521 gcttcacgtc gggcctggtc gccgacattc aggttccgca gctcgagaca cgtgtcgcga
     5581 tcgtaaagaa gaaggcgcag ctcgagggca tcgacctgac cgacgacgtg gcggtgctgc
     5641 tcgcacagaa tgtgcaaagc aacatccgcg agctggaagg cgcgttgatc cgtctggccg
     5701 ccaagtcatc actcacgggg cgccccatcg atgaggagtt cgcgcgcagc gagctgtccg
     5761 tcgtcggtcc gcgtcggccc gacctgatga gcgtggagga catccagcgc gcggtgtgca
     5821 gtcacttccg cttgtccaac gcggagcttc tgagcaaaga ccgccacaag agcgtcgcct
     5881 tcgcgcggca ggtggcgatg tacttgtgcc gtcaacggct caagtgctcc ttcccggagc
     5941 tcggacgcgc cttcggcaat cgcgaccaca ccacggtcat gagcgcggtg cgtcgagtgg
     6001 aagcgctccg tcagcgcgac ccgcaggtca acgcacacct cgaagcaatc gagcaacgcc
     6061 tcgcctcctc cgaagagtaa acgagcaagc tccgagctcg atcggcagag ctcaggttca
     6121 gagctcgacc aatcaaggcc cagcgaggtt caagactcgg ctgggccttt gacttgggag
     6181 agcaaaacct tcacggagga gtcgctcgcg gcatcggtga tgaccgtgac gtaggtctca
     6241 ttctccggga agaccgtatc gactcggact attttgcccg cacggccgtc gtccaacacg
     6301 acgcgcctgc gatcgttcat atagcgatgg atgtggcgca gcgtcgtcac agctcgagga
     6361 tagcgcaggc gtccgctgcg caccccctgg aatgggccac aggtcgaaat ggtggccggc
     6421 ccgcgcggcg cctccgtcga tgccgttttt ttgccggcgg tggggcaacc tcctatcgtt
     6481 gcgccgtcat gtctctccgg actcaatgca gtcggcgcct cgggacccta atcccgcgct
     6541 ggtcgacgcc caccgcggcc gcgctgctcg ggctacatct cttgtgcggg tgttcgctcg
     6601 ctcacacggc caccgtgcgc accgcggcgc tccccccgcc cagcgaacct gtgattcgtc
     6661 attcggctcg gctctggctc gacatctttc caggagtcaa tgggacgcag tgtgtcatgc
     6721 ctccgcaagc cactcgtgag gtctgcttcg atgcggtgga ggacgccgtt caggcctccc
     6781 tctcccgagt gctttggacc agcttcccgc gagtcgatgt gctgggacgc ggagataccc
     6841 cggccccgga agactacgtg cttcacctgg at
//
LOCUS       DASTKU010000241         8757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1387337, whole genome shotgun sequence.
ACCESSION   DASTKU010000241 DASTKU010000000
VERSION     DASTKU010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387337"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFQ61_08830"
     CDS             <1..343
                     /locus_tag="VFQ61_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954596.1"
                     /translation="VPIVPIVIDGTGSILAKGTVGIRPQAVIVRVLEPIHAAPGERDG
                     RSLQRRVFERMQRELDLIRRAEPGHAEAMHADAKHADSTSLPALPTARAGKATPAGTE
                     DAATQDAFEPS"
     gene            547..2106
                     /locus_tag="VFQ61_08835"
     CDS             547..2106
                     /locus_tag="VFQ61_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HET9954597.1"
                     /translation="MRNPLKLHNVPGGRPYMPTYVENPFAEENPFRTKPRRNIEPWPS
                     FAESRALLPQPYYPARPAAVDCYFRCWQIAFDNFRKATEKNGFVSDYSSTMFADCLFM
                     WDSVFITLFGRYADRVWRFQNTLDNFYVKQHPDGGICRQFLEDDGSECYARFDPAGTG
                     PNILGFSEWEYYLFTRDEQRLAEVFPALVAYGQWFRRYRTWPNGSYWGTGWASGMVNQ
                     PRTPAGAHLWYDHAHRTWVDTNLQQVLANRIVLQMADALGRRAEVRDVAAENAALVPW
                     INEHLWDEGTGFYHDLERDQSRITAVKTIGAYWALLADAVPPARLARFVSHLENPSEF
                     ARKHCVPSLSADTAGYDPDGGLWLGGVWAPTNYMVLSGLLRHGYVDLAYRLASNHFQN
                     VMNCYERTGSLFEHFAPDQSAQGRGRKDFVGWTGITPIAMFFEFILGLQPQPAQQRLV
                     WNLWNTDELGVDRYPFGQHGSLELRASARASASDRPVVSIKSNVPLTLELVWQGGRET
                     RRVMPDDRSSP"
     gene            2134..3087
                     /locus_tag="VFQ61_08840"
     CDS             2134..3087
                     /locus_tag="VFQ61_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET9954598.1"
                     /translation="MNPSSPQLSTPAIASSSSPRAMVSLDGILHDAAGAQVSVFDRGF
                     LYGDSVFETLRTYFGRPYALQEHLQRLENSAKRVLIPMPVPRDVWCREIEQTIAAAGF
                     SESYVRVMLTRGRGEQLGLDPALARQPLRLVLVLPLEAPVAHKYERGIGAIIYRTQRV
                     ADGTDAAGAKLGNYLTAVLATHAARLAQAEEALLVDREDQVLEGATSNVFGVASGHLI
                     TAPEECGILAGITRARILAVAHQLGLPVVMRPLRVAELATLNELFISSSIREIFPVVS
                     VDGDPIRDGHPGPITRELLGAFRARARAEVRGEPVEAEPEA"
     gene            3298..4239
                     /locus_tag="VFQ61_08845"
     CDS             3298..4239
                     /locus_tag="VFQ61_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954599.1"
                     /translation="MPSLLSPLRSLALPVTVLAALSQGCVAEKCETADGKEATCISVP
                     DRFEAPTLEQTLTGDYEPGSDLRIKGVYGDINVRAGTAGEVSLRVRPFAYGTHDSKDE
                     AFEKMRDKLYAEIANDGNAVAITTWRDDTKLPLVGADLDIYLPPEFDGGLVVENQGNG
                     TWNPGNIKVFEAAEARSVEVRATSLSDCQLQLGPTVTYTAVRCDGDIRVVGASDDVSL
                     ETQGQLGNIEVQLVNIEPTSSGGRFKAGSDIRVIFPSGDDFSVQATGGKLVDFGGLPA
                     ASCSVEAAAENSKSATCGDGGPLYEITSLDGTVKASF"
     gene            complement(4329..5570)
                     /locus_tag="VFQ61_08850"
     CDS             complement(4329..5570)
                     /locus_tag="VFQ61_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954600.1"
                     /translation="MSDTSKLTQPDLDGRWLSADAQTALAVAAEAGARAAALVDAWVK
                     AGNAAAVATLADRGEGAARKAARRGLNVLKSRGIRVEAPPRVVPVAGDAREPELQTEA
                     WMVPPDAEGNTLIVLASRTQTSRAQSAFITVRDSAFVLGVNVGELSGSRLKEALKRAA
                     QGAEPVRITPEYARFRIAEARKQQKQLGIPEPLGTTSAQKLLEPVPSGPVPHPLENEG
                     LELSEEDCKELAGKSGALHGLREFQPWLPDRSSVEEMLREVGRKLPRENGQPKEEAIE
                     PLLTECVAEATDRYFIPERRRVLVDRLKDCALSVLAREGEVRALELVAVIKEIEKAGL
                     ITDPPRDIPFLRAFFEKAVAMLAMRGGGRLQIPLSGPGPDGASEATASGGAEGASSAQ
                     DSPAQENPSGEAAASSTETNG"
     gene            complement(5619..6005)
                     /locus_tag="VFQ61_08855"
     CDS             complement(5619..6005)
                     /locus_tag="VFQ61_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET9954601.1"
                     /translation="MPKTIVVSEQAPKPVAAYSRAVRAGDFLFLSGQIPIDPATSELV
                     AGDIQAQTRRVLENLRAVLAAANAGFQNVVKVSVFLTDMQDFAAFNAVYVTYFETEPP
                     ARATVAVSALPRGARIEIEAVAYLGA"
     gene            complement(6021..6629)
                     /gene="recR"
                     /locus_tag="VFQ61_08860"
     CDS             complement(6021..6629)
                     /gene="recR"
                     /locus_tag="VFQ61_08860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET9954602.1"
                     /translation="MLPERLRRVIDLLSRLPSVGEKTAQRFALYLAANPTGLAAELGQ
                     ELALLAQEVKPCERCGNLAQVADGHAICPICRDSRRDSRILCIVHRVQDLLAIERSGA
                     LRGRYFVLGKLLSPLDGVGPDELPLAELTRRIREDGVEEVLLATPLSVDGEATALLLS
                     RELSALGVRVTRIASGVPHGGDLEFADQVTLGRAIDGRRSMG"
     gene            complement(6670..>8757)
                     /gene="dnaX"
                     /locus_tag="VFQ61_08865"
     CDS             complement(6670..>8757)
                     /gene="dnaX"
                     /locus_tag="VFQ61_08865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET9954603.1"
                     /translation="ALTCRRDVGPTPEPCLTCPACTEIAQGTDLDVREIDGASYNGVD
                     EVRKLQDSIPYRPSRDRFKIFVVDEVHMLSQSAWNAFLKTLEEPPPHVKFIFATTEVH
                     KVPITILSRVQRFDFRMIGAKVVAERLRYVLEQEKIEADDAAISILSREAAGSMRDAM
                     SLLDQVIAWGGDTLRGEDVARVLGVASHKVLYELSRALLQGDPALCLGIVADLANQGH
                     DMTHVARDVLQLLRDLVVAKVCENPGSLLDLPDEEAREVKALAVPADTDDLLRLHQGF
                     GEGFDDIARSGQPRAALEMLLVRLSRRPPLLPVDGMLQRLTQLEHRLHGGGAGPDRRR
                     AAAPAAPNAVRPGSAPGVPASSTPANRPREPRSASGPRVEGGDDRKEPPRELPRPAIL
                     GPEVAADSHAAGFDAAPIASIGYPARVSPGAGEHAGKTASLPPSGPALRADATSPQAP
                     RAVPSTLPPSSLSIATSGVATTASPSGSAVNGSHANGSAASSAPTLPRKTDARRDAAP
                     LPEFQAPAWTRQEPTRSATRGDSGRSAGRNGHASAPAPNAEPPSLASVPSTPEALDDF
                     LRKLVGRIASERPEIAAKLEHSVLLECQRDRVLFGWAKDSLFGNLVATTENREVIERF
                     ASGLLGGKVRVEHEIDSPRAAGKKTLSVLEVEARERRVREAYEQARSHPRIAEAIEVL
                     GARLKELKLARIP"
BASE COUNT         1531 a         2755 c         2873 g         1598 t
ORIGIN      
        1 ggtgcccatc gttccgattg tgatcgacgg cacaggatcg atcctcgcga agggtacggt
       61 cggaattcgc ccccaggcgg tgattgtccg cgtgctcgag ccaattcatg ctgcgccagg
      121 cgagcgcgat gggcggtcgc tccagcggcg cgttttcgag cgcatgcaac gtgagctcga
      181 tctgatccgc cgggcggaac cagggcacgc agaggcgatg catgcagacg cgaagcatgc
      241 agactcgacg agcttaccag ccttgccaac cgccagggca gggaaggcca cgccggccgg
      301 aaccgaggac gcggcaacgc aggatgcgtt cgagccatcc tgatgccgcg tccgatgccc
      361 gctcactgac cgatgcagtc gagcttgcat gctttcgatg cgagcttgga tgcgcgtgct
      421 gagccgtaaa gaccgatttc agcgatccag ccagggatcg aaatgatccc gagcagggcg
      481 gggtcgaagg tgaaacagtg tcccttgtgg tatggctcga gacggccgtc gtgaggctgt
      541 tccccgatgc gcaacccgct caagctccac aacgttcccg gtggtcggcc gtacatgccg
      601 acctatgtcg agaatccgtt cgcggaagag aaccctttcc gcacgaagcc gcgccgaaat
      661 atcgagccat ggccgagctt cgctgaatcc cgagcgctgc tcccgcagcc gtactatccg
      721 gcgcggccgg cggcggtgga ctgctacttt cgttgttggc agatcgcctt cgataacttc
      781 cgcaaggcca ccgagaagaa cggcttcgtc tcggactact cctccacgat gtttgccgat
      841 tgcctcttca tgtgggactc ggtgttcatc acgttgttcg gtcgctatgc cgatcgggtg
      901 tggcggtttc agaacacgct cgacaacttc tatgtgaagc agcatccgga cggcggcatt
      961 tgtcggcaat tcttggagga tgacggcagc gagtgctacg cgcggttcga tcccgcgggg
     1021 acgggaccaa acatcctcgg cttcagcgag tgggaatact atctgtttac gcgcgacgag
     1081 cagcgactag cggaggtctt tcccgcgctc gtggcctacg gtcagtggtt tcgccgctac
     1141 cgcacttggc cgaatgggag ctattggggc accggctggg ccagcggcat ggtcaatcag
     1201 ccgcgcactc cggcgggggc gcacctctgg tacgaccatg cgcaccgcac gtgggtggac
     1261 accaacttgc agcaggtatt ggccaaccgc atcgtgctgc aaatggccga cgcgctcggg
     1321 cgccgcgcgg aggttcgcga cgtcgcggcc gaaaacgcgg cgctcgtccc ctggatcaac
     1381 gaacatttgt gggacgaagg cactggcttt taccacgatc tagagcgcga tcagagccgc
     1441 attaccgccg tcaagacgat cggtgcgtac tgggcgctac tggcggatgc ggtgccgccg
     1501 gcgcgactcg cgcgcttcgt ttcgcacctc gaaaatccat ccgagttcgc gcgtaaacac
     1561 tgcgtgccca gcctttcggc agacaccgca ggatacgatc cagacggcgg cttgtggctg
     1621 ggcggagttt gggctcccac caactacatg gttctgagcg ggttgttacg ccacggctac
     1681 gttgatctgg cttatcgctt ggctagcaat cacttccaga acgtgatgaa ttgctacgaa
     1741 cgcaccggca gtctgttcga gcactttgcg cccgatcaat cggcgcaagg gcgcggtcgg
     1801 aaagacttcg tgggctggac gggcatcacg cccattgcca tgttcttcga gttcattttg
     1861 gggctgcagc cacaacccgc acagcagcgc ttggtttgga atctttggaa caccgacgag
     1921 ctcggcgtcg atcgttatcc ctttgggcag cacggaagtc tggaactccg agcgagcgcg
     1981 cgagcgagcg ccagtgatcg ccccgtcgtt tctatcaaga gcaacgtacc gttgacgctc
     2041 gagctggttt ggcagggcgg gcgagagaca cgccgcgtga tgcctgacga tcggtcgagc
     2101 ccatagcctt gcacgctgtt ttcaggagtg aacatgaacc cgagctcgcc tcaactctcg
     2161 actcccgcca tcgcgagctc gtcctcgccg cgcgcgatgg tcagcctgga tgggatcttg
     2221 cacgacgcgg ccggggctca ggtcagcgtc ttcgatcgcg gatttctgta cggcgactcg
     2281 gtgttcgaga cattgcggac ttacttcggc aggccctacg cgctgcagga gcatctgcag
     2341 cgtctcgaga atagcgccaa gcgagtgctg atcccgatgc cggtcccgcg cgacgtttgg
     2401 tgccgcgaga tcgagcagac gatcgccgct gccggctttt cggagagcta cgtgcgagtg
     2461 atgctgaccc gggggcgagg cgagcagctc gggctcgatc ctgctctagc gcgccagccc
     2521 ctccgactcg tgctcgtact gcccctcgaa gcacccgtgg ctcacaagta cgaacgaggc
     2581 atcggcgcga tcatttaccg gactcagcgc gtggccgatg gtaccgacgc cgcgggcgcg
     2641 aaactcggaa attacctcac cgcggtgctg gccacgcacg cggctcggct ggcgcaagcc
     2701 gaggaagcgt tgttggtgga ccgtgaagat caggtgttgg aaggcgcgac ttccaacgtg
     2761 tttggcgtcg cttcggggca tctgattacc gcgcccgagg agtgcggcat actcgcgggg
     2821 attacgcgcg ctcgcatcct agccgtagct caccagctgg gcctacccgt ggtcatgagg
     2881 ccgctccggg tggctgaact cgcgaccttg aacgagctct tcatttcgtc ttcgatacgc
     2941 gaaattttcc cggtcgtgag cgtggacggc gatcccatcc gcgatggtca tccggggccc
     3001 atcacccgcg agctcctggg cgcgttccgg gctcgagccc gtgccgaggt gcggggagaa
     3061 cccgtcgagg ccgagcccga agcttgatgg ggcatttcgg accaggtcga accagccagc
     3121 ggggccggcc attgaagata gctcgttggg tccgggcctc gatttcgagt cggctccagt
     3181 tcgagtcggc tccagtttga gtccgagggg cggctgcact acacagcacc tgcgacccaa
     3241 atgggtcctg ggaagatcca ggaatcgctg acgtaagata tcgccaagga tacgaagatg
     3301 ccttcacttc tctcgccgtt acgttcgttg gctttgcccg tcaccgtgct cgccgcgttg
     3361 tcccagggct gcgttgccga aaagtgcgaa accgcggacg gtaaggaggc gacctgtatt
     3421 tcggtacccg atcgcttcga agcgcccact ctcgagcaaa cgctgacggg ggattacgag
     3481 cccggctcgg atctgcgcat caaaggcgtt tatggcgaca tcaacgtgcg agccggcacc
     3541 gcgggagagg tttcgctacg ggtgcgaccg tttgcctacg ggacccacga ctcgaaggat
     3601 gaagccttcg agaaaatgcg tgacaagctc tacgcggaga tcgccaacga cggcaatgca
     3661 gtcgcgatta ccacctggcg cgacgacacc aagttgccgc tggtcggggc agatctagat
     3721 atctatttgc cacccgagtt cgacggcggg ctcgtcgtag agaatcaggg caacggcact
     3781 tggaacccgg gaaacatcaa ggtcttcgag gcggcggagg caagatcagt ggaggttcgc
     3841 gccacttcgc tgagtgattg ccaacttcaa ctgggcccca ccgtgacgta cacagccgtg
     3901 cgctgtgatg gtgacattcg cgtggtcggt gcgagcgatg acgtgtcgct cgagacgcag
     3961 gggcagctcg gcaatatcga ggtgcaactc gtgaatatcg agccgacgag cagcggcggc
     4021 cgcttcaaag caggctctga catcagggtc atcttcccct cgggcgacga cttcagcgtg
     4081 caggcgacgg gcggcaagct ggtcgatttt ggcggcttgc cggctgcaag ctgttcggtc
     4141 gaggcagcgg cggagaactc gaagagcgcc acctgcggag atggtgggcc gctctacgag
     4201 atcacgagcc tcgatggcac cgtgaaggcg tccttctgat tcgcgacgac aggtgattcg
     4261 cggcggcaga tgagcccaac ggatcggctc agggcgagga aactcgccct gagcactgcg
     4321 taaggctctc agccgttcgt ttccgtcgag ctggctgcgg cctcgccgct cggattctct
     4381 tgcgcgggcg aatcttgggc ggacgaagcc ccttcggcgc cgcccgaagc agtggcttcc
     4441 gacgcgccgt ctgggccggg accggaaagc gggatctgca gccgtccgcc gccgcgcatg
     4501 gcgagcattg ctacggcttt ttcgaagaag gcgcgcaaga aggggatgtc gcgcggcgga
     4561 tcggtgatca gccccgcctt ctcgatctcc ttgatcacgg cgacgagctc gagggcgcgc
     4621 acctcgcctt ctcgggcgag cacgctcaac gcgcaatcct tcaggcggtc gaccagtacg
     4681 cgccgacgct ccgggatgaa gtaccggtca gtcgcctcag ccacacactc ggtgagcaaa
     4741 ggctcgatgg cttcttcctt gggttggccg ttctcacgcg gcaacttgcg ccccacttca
     4801 cgcaacatct cctcgaccga gctccgatcg gggagccagg gttgaaactc gcgaaggccg
     4861 tgcagcgcac cggacttgcc ggcgagctcc ttgcaatcct cttcggagag ctccaaccct
     4921 tcgttctcga gcgggtgggg aacggggcca ctcggtaccg gctcgagcaa cttctgcgcg
     4981 gaagtggtgc cgagcggttc ggggattccg agctgctttt gctgcttgcg tgcttcggcg
     5041 atgcggaagc gtgcgtactc aggtgtgatg cgcacgggct cggcgccttg cgcggcgcgc
     5101 ttcaaggctt ctttcaggcg gctgccgctg agctctccaa cattgactcc gagcacgaac
     5161 gcgctgtctc gcacggtgat gaaggcgctc tgggcgcggc tcgtttgcgt gcgcgacgcg
     5221 agcacgatca aggtgttccc ttcggcgtcg ggcggcacca tccacgcttc ggtttgcagt
     5281 tccggttccc gcgcgtcacc ggccacgggc actacacgcg gcggcgcttc cacgcgaatt
     5341 ccgcgtgatt tgagcacgtt caggccacga cgcgccgctt tgcgggcggc tccctcgccg
     5401 cgatcggcca aggtggctac cgcggccgca ttgcctgctt taacccatgc gtccactagg
     5461 gccgcggcgc gcgcgccggc ttcggcggcg acggcgaggg ccgtttgggc gtcggcggaa
     5521 agccagcgcc cgtccaaatc aggctgcgtc aacttgctcg tatcggacat ctcgtctccc
     5581 ggatttcgcg cccctgattc ggggctcagc gtcgaggctc acgccccgag gtaggctacg
     5641 gcctcgattt cgattcgggc gccccggggc agggccgaaa cggccacggt agcgcgagcc
     5701 ggtggctcgg tttcgaagta ggtgacgtac acggcgttga aggccgcaaa atcttgcatg
     5761 tcggtcaaga acacggagac cttgacgacg ttttgaaaac ccgcgtttgc cgctgccaac
     5821 actgcccgca ggttttcgag cacgcgccgc gtctgggctt gaatgtcccc cgcgacgagc
     5881 tcggacgtag ccggatcgat cggaatctga cccgacagaa ataagaaatc tcccgcgcgc
     5941 acggcccggg aatatgcagc cacgggtttg ggggcttgct ccgagacaac gatggttttg
     6001 ggcattggcg ggcgcgcaga ctaacccatc gagcggcggc cgtcgatggc acgccccagg
     6061 gtcacttgat cggcgaactc caggtccccg ccatgcggaa cgccactcgc aatgcgcgtt
     6121 acacgcacgc cgagcgcgga cagttcgcgc gacagcagca gggccgtggc ttcaccgtcg
     6181 acggacagcg gggtggcgag caacacttct tcgacgccat cttcacgaat gcggcgcgtg
     6241 agctcggcga ggggcagctc gtccgggcca accccgtcga gcggcgatag taatttacca
     6301 agaacgaagt aacgcccgcg cagcgcgccg ctccgctcga tggcgagcaa atcctggacg
     6361 cggtgcacga tgcagagaat gcgggagtcc cgccgggaat cacggcaaat tgggcagatc
     6421 gcgtgcccgt cggcgacctg cgccaggttt ccgcatcgct cgcagggttt gacctcctgc
     6481 gccaacagcg cgagctcttg accgagctcg gcggcgagtc ccgtcgggtt ggccgccaaa
     6541 tacagcgcaa aacgttgggc cgtcttctct ccgaccgagg gcaaacggga aagcaggtcg
     6601 atcacgcgtc gcagtcgctc aggtaacatc gcaggtgctc acaaccaatc cgtttgcagc
     6661 gtgacgttat cagggaatgc gagcaagctt cagctccttc aggcgcgctc ccagaacctc
     6721 gattgcttcc gcgatccggg gatggctgcg ggcttgctcg taagcttcac gcacccggcg
     6781 ctcgcgcgcc tccacctcga gcacgctcag cgtcttcttg cctgccgcac gcggcgagtc
     6841 gatttcgtgc tcgacccgca ctttgccacc caacaggccg ctcgcgaaac gctcgatgac
     6901 ttcccggttc tcggtggttg cgaccaggtt cccgaaaagg ctgtctttcg cccacccgaa
     6961 caacactcgg tcgcgttgac actcgagcag aacgctgtgc tcgagcttcg cggcaatttc
     7021 gggtcgctcg ctggcgattc tccccacgag cttgcgaaga aagtcgtcca gtgcttcggg
     7081 cgtactcggc acgctcgcga gtgaaggcgg ctcggcgttg ggcgcgggcg ccgaggcgtg
     7141 accattgcga ccggcagaac gcccggagtc gccgcgtgta gcggagcggg taggttcctg
     7201 ccgagtccag gcaggcgctt ggaactcggg tagaggtgcg gcatcgcgac gggcgtcggt
     7261 tttgcgcggc aacgtcggtg cggaggaagc ggcggagccg ttcgcatgag agccattcac
     7321 ggccgagccg ctgggggatg cagtcgttgc gactccactc gttgcgatgg agagagagct
     7381 gggtggcagt gtgctcggga ctgcacgtgg cgcttgcggt gaggtggcat cggccctgag
     7441 cgcgggcccg gagggcggaa gcgacgcagt ctttcctgcg tgttcgccag ccccgggtga
     7501 aacgcgggcg gggtacccaa tggacgcaat gggggctgca tcaaagccgg cggcgtgcga
     7561 gtctgccgcg acttccggtc ccaatatcgc gggtcgcgga agctcccttg gaggctcctt
     7621 tcgatcatct cccccttcga cgcgcggccc ggaagcggag cgcggctcgc gggggcgatt
     7681 ggcgggggtc gaagaggccg gaactccggg cgccgagcct gggcgcacgg cgttcggagc
     7741 cgctggagcc gcagctcgac gtcgatcggg tccagctcca ccaccgtgca gacggtgctc
     7801 gagctgagtg agccgctgga gcatgccgtc gactggcaac agcggaggcc gtcgcgagag
     7861 acgcaccaac aacatttcca gcgccgcccg cggttgcccg ctgcgcgcga tgtcgtcgaa
     7921 gccttccccg aagccttggt gcaggcgcaa caggtcgtcc gtgtccgcgg gcaccgcgag
     7981 cgctttgacc tctctggctt cctcgtccgg aagatccagc aagctgcccg ggttctcgca
     8041 caccttcgcc accaccaggt cacgcagcag ctgcagcacg tcgcgcgcca cgtgggtcat
     8101 gtcgtgccct tgattggcca gatccgcgac gatcccaagg cagagtgccg ggtcaccttg
     8161 cagcaaagca cgtgagagct cgtagagcac cttgtggctc gcgacgccca gcacgcgcgc
     8221 cacgtcctcg ccgcgcaagg tatcgccacc ccaggcaatg acctgatcga gcaagctcat
     8281 ggcgtcgcgc atgcttcccg ccgcttcgcg ggacaagatg gagatcgcgg cgtcgtcggc
     8341 ctcgatcttc tcttgttcga gcacgtaacg cagccgctcc gccaccacct tggcgccgat
     8401 catgcgaaag tcgaagcgtt gaacgcgcga caaaatcgtg atcgggacct tgtgcacctc
     8461 ggtggtcgcg aagatgaact tgacgtgtgg tggtggctct tcgagcgtct tcaagaaggc
     8521 gttccacgcg ctctgcgaga gcatgtgtac ctcatcgacc acgaaaatct tgaagcggtc
     8581 gcgcgagggg cggtacggga tgctgtcttg cagcttacgc acctcgtcca cgccgttgta
     8641 actcgcgccg tcgatctcac ggacgtccaa gtcggtgcct tgagcgatct cggtgcaggc
     8701 cgggcaggtg aggcagggtt caggggtagg gcccacgtcc cgcctgcaag tcagcgc
//
LOCUS       DASTKU010000242         5333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1387355, whole genome shotgun sequence.
ACCESSION   DASTKU010000242 DASTKU010000000
VERSION     DASTKU010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387355"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1843)
                     /locus_tag="VFQ61_08870"
     CDS             complement(<1..1843)
                     /locus_tag="VFQ61_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9954604.1"
                     /translation="MSAQQAKILIVEDDVRAADELSEILRALGFEVSAKAACAEEAIA
                     AASTNPPDLVLLDVGLEGTRDGIETAAILRHQYRLPVVFLTAYSDPHTLQRAKDAEPY
                     AYLIKPVKVNDLRSTLTLALHRAERDRSTREQTTWLTAMLDSLSDAVVGVTGTDHITY
                     MNRSAELLFGCVAGEMKGRPLSELELRTEATNGDATYDTVRTTMLDTQGRALGALLVH
                     RPALRARTPTLRPTQPSPSMTLPSIPIPTMPLPSIMGLRAGASGIEPERAAARASTRD
                     GGATDGGSHDGGATDGSSAGSPRDRASGDGAPVSSTPPDGAESWSSFSAREAQAKGNG
                     AGPSAMPPSPFPASPQLATLVTGVAQEVNNPLAYVVVNQEYAIQELLKLEARLEADLP
                     GGDGTEGQAPPSPRSSEPSEVRHEEAPSGSHRIADTRSMLQNVRSALSDAQMGAARVR
                     TAVAELSQLNPLETRPPPGAPQQRPSGSYQLDPEVSLPMSGRVPSSRLKRILLVDDER
                     PLLEAMARMLGSAHYIARVTSAEAALALLAEDSEFDLVLSDVVMPSMDGLQFYAEVER
                     RHPELAKRFAFMTGGVLSNEVRSFLTSGRCPLLLKPFSHEELREFIGN"
     gene            complement(2298..2963)
                     /locus_tag="VFQ61_08875"
     CDS             complement(2298..2963)
                     /locus_tag="VFQ61_08875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9954605.1"
                     /translation="MIAVIVILIVSAGAHAFVFRESVAGSPAFWWGLCVPYTLLDLLA
                     LHQLRRRKRLDALMPRFGDLSIGVSTGALLLFASFLCRSTLSPTGTPRQAWLYRIFLQ
                     VGDPEVIQHSAILTLAVLFIACSEELVWRGAVLEILSERLGSRPAWLVTTLAYTAVML
                     PSLFALRDPIAGLNPLLPIAALGAGLVWSFLAARQRRLPPVMISHMVFSYFTIVQFRW
                     PTL"
     gene            complement(2960..4246)
                     /gene="coaBC"
                     /locus_tag="VFQ61_08880"
     CDS             complement(2960..4246)
                     /gene="coaBC"
                     /locus_tag="VFQ61_08880"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET9954606.1"
                     /translation="MSSLRRPPLERYPEPFPSPAPEPRLRGKRIVLGVTGSIAAYKAV
                     AVLRLLLKEGADVEVVLSAAATQFVGDATFSGLTGRPAYQAMFDPSRGGELHVDLARG
                     CDLFLIAPATADFLARMSTGRADDLLGALALCVTCPVLVAPAMHPNMWKHPATQRNWQ
                     ALEAQGRVQRIGPVSGPVASGDIGEGRLSEPEDIVTRVVAELLEPTWLGKHLVVTAGP
                     TVEAVDPVRLLTNRSSGKMGFALAARAALRGATVTLISGPVALPTPLGVKRIDVRSAL
                     DMERALDLALGADLDRADALVMCAAVADYRAARVAPDKLDRSAGPTQLELTPNPDLLA
                     ALGARRKSRTPVLVGFALQTGDWTHTVEQARAKLERKKVDMIVANRAEETLESSTIAA
                     RLITASDVEELTTLSKAHAADLILERVRALFDEATR"
     gene            complement(4259..4960)
                     /locus_tag="VFQ61_08885"
     CDS             complement(4259..4960)
                     /locus_tag="VFQ61_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET9954607.1"
                     /translation="MRLTRDFYARPVLVVARDCIGKVLVHETPAGRLRGRIVETEAYA
                     GPEDRAAHSYGGRRTARTEVMYGPPGHVYVFFVYGMHWNFNLVTGRAGEPQAVLVRAL
                     EPLEGFAEIARRRGASKPWERMSMRERCALTNGPGKLCQAFGIDRAAYGADLETGPLY
                     LDDGTFDGSALDAGASQGTAADGSALTRRSKPRVSRSARIGVDYAGEWALKPYRFYDA
                     ESAFVSVSRATRDRV"
     gene            complement(5012..>5333)
                     /locus_tag="VFQ61_08890"
     CDS             complement(5012..>5333)
                     /locus_tag="VFQ61_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954608.1"
                     /translation="AFAAREVFVSTLGLVYGLEDLDDEAKPLREKMREERTSDGKPRY
                     TPLIGLSLMLFFALSCQCVSTLAVVRRETRSWRWPAFMFGYMTLLAYATSFLVYQGGR
                     LLGF"
BASE COUNT         1008 a         1712 c         1698 g          915 t
ORIGIN      
        1 aattgccgat gaattcgcgt aactcctcgt gagaaaacgg cttgagcagg agcgggcaac
       61 gaccgctcgt caagaagctc cgcacttcat tgctgagcac tccgccggtc atgaacgcga
      121 atcgcttggc cagctctgga tggcggcgct ccacttccgc gtagaactgc agaccatcca
      181 tactcggcat caccacgtcc gacagaacaa ggtcgaattc gctgtcctcc gcgagcaacg
      241 cgagcgccgc ctccgcgctg gtcacccgcg caatgtaatg ggccgatccc aacattcgcg
      301 ccatggcctc cagcagtggc cgctcgtcat cgaccaacag aattctcttg agacgcgagg
      361 aaggcacgcg gcccgacatc ggtagcgaca cctcaggatc caattgatag ctgcccgagg
      421 gacgttgttg gggcgccccc ggcggaggtc gagtttccag cgggttgagc tgtgagagct
      481 cagccacggc cgtacgcacg cgcgcggctc ccatttgcgc gtcgctgagc gcggagcgca
      541 cgttctgcag catcgaccgg gtgtcggcga tgcgatgcga gccgctggga gcctcttcgt
      601 ggcgaacctc gctcggttcg gaactgcgcg gcgagggagg cgcttgaccc tccgtcccgt
      661 cgcctcccgg caggtctgcc tcgaggcgag cttcgagctt caagagctcc tgaattgcgt
      721 attcttgatt caccaccacg taggcgagcg ggttgtttac ttcttgcgct acgccggtga
      781 ccagagtcgc gagctgcggc gacgctggga aagggctggg gggcattgcg ctgggtcccg
      841 ctccgtttcc tttcgcctgg gcttcacgtg cggagaacga gctccaggac tccgcgccat
      901 cggggggcgt agagctgacg ggcgcgccgt cacccgaagc ccgatctcgt gggctgcccg
      961 cggacgagcc gtcagtggca ccgccatcgt gggagccacc gtcggtagcg ccgccgtcgc
     1021 gcgtcgacgc tctcgcagca gctctctccg gctcgatacc gctcgccccc gctctcagcc
     1081 ccatgatgct gggtagcggc atggtcggga taggaatgga gggcaaagtc atgctcggac
     1141 tcggctgcgt cgggcgcagc gtcggagtgc gtgcccgcaa tgctggccgg tgcacgagca
     1201 aggcgccaag agcacgcccc tgggtgtcca acatggtggt gcgaacggtg tcgtacgtgg
     1261 catcgccgtt cgtggcctcc gttcgaagct ccagctccga gagtggtcga cccttcatct
     1321 ctcctgccac gcagccgaac agcagctccg cggagcgatt catgtacgtg atgtggtcgg
     1381 tgcccgtgac tccgacgacg gcgtcgctca gcgagtccaa catcgcggtc aaccaggtag
     1441 tctgttcgcg cgtcgatcgg tctcgctccg cgcgatgcaa tgccagcgtg agcgtcgagc
     1501 gcaggtcatt cactttgact ggcttgatca ggtaagcgta cggttcggca tccttggcgc
     1561 gttggagcgt atgagggtcc gagtaagcgg tcagaaacac gaccggcaat cgatactggt
     1621 ggcggagaat ggcggccgtc tcgatcccgt cgcgcgtgcc ttcgagcccg acgtcgagca
     1681 gcacgagatc gggagggttg gtgctcgcgg ccgcgatcgc ttcctcggcg cacgcggctt
     1741 tggccgagac ctcgaaaccg agcgcgcgga gaatctcgga tagctcgtcg gcagcacgaa
     1801 catcgtcctc gacgatcaga atcttggctt gttgggcgct cactcggtcc ctcgcagggg
     1861 gaccgaactt ggcttttacg ttcgtccgat cgacatgcgt tcggtcgatg aacgatcgag
     1921 cgcgtcggtc ccctcggcgc actgagcgtg actgatgatc gtgtgcagaa tagagtgttc
     1981 ccctcgtggt ttccacgccc gtgaggactg caacggtaga ttttccggat tggtcaaaca
     2041 cgcaatgtgg ttttgtgcca caggtgggtg gttgaggcgt tttatacagt gagtttcgag
     2101 gcgagctggg catggcaagg aagggcacct cgcgtgattg tggtcggggt tcccggtctg
     2161 gccggcctcg acgcggctcg ctccagattg gaaacgagcc gagtctcatg ggtgcccagc
     2221 gttcctctat tcacgatgcg ggccacattc cctcgtccga cactcgcgct caaattggga
     2281 cgcgagcgcg ttacccctca cagcgtcggc caacgaaatt gcacgatcgt gaagtagctg
     2341 aagaccatgt gagagatcat caccggaggc agtcggcgct gccgcgcggc aaggaaactc
     2401 caaaccaaac ctgcgcccaa cgcggcgatt ggcagcaacg gattgagtcc cgcaatggga
     2461 tcgcgcagcg caaacaagct gggaagcatg acggcggtat acgcgagagt cgtgacgagc
     2521 caagccggac gtgagccgag acgctcagag agaatttcta ggacggcgcc gcgccacacc
     2581 agttcctccg aacaggcgat gaacagtacg gccaaggtca ggatcgcact gtgttgaatg
     2641 acttcagggt cacccacctg gaggaagatt cgatacaacc aagcctgtcg cggcgtgccg
     2701 gtgggcgaga gagtgctcct gcacagaaac gacgcaaaaa gcagaagcgc ccctgtacta
     2761 acgccaatcg ataaatcccc gaaccggggc atcagcgcgt ccagtcgctt ccggcgtcgg
     2821 agctggtgca gcgccaagag gtccaacagc gtgtaaggca cacacagtcc ccaccaaaat
     2881 gccggactgc ccgcgacgct ttcgcgaaac acgaaggcgt gtgcgcccgc actcacgatc
     2941 agaatgacga tcaccgcaat catcgggtcg cctcgtcgaa caatgcccga acgcgctcga
     3001 gaatgagatc ggcagcatgt gctttggaca aggtagtgag ttcctcgacg tcggaggccg
     3061 tgatcaatcg agcggcaatc gtcgaggact cgagcgtctc ctcggctcgg ttagcgacga
     3121 tcatatcgac tttctttcgt tccaatttgg cgcgcgcttg ctcgacggtg tgcgtccaat
     3181 cgccggtctg caacgcaaag ccgaccagca ccggcgtacg gctcttgcgt cgtgcaccca
     3241 gtgcagccag caggtccgga ttgggcgtta gttcgagttg agtagggccc gcgctgcgat
     3301 ccaacttatc gggtgcaacg cgcgcggctc ggtaatccgc gacggcggcg cacatcacga
     3361 gcgcgtccgc tcgatccaga tcagcgccca gcgccagatc caacgcgcgc tccatatcca
     3421 gggcgctgcg cacgtcgatg cgcttgacgc cgagcggcgt gggcagcgcc accggaccac
     3481 tgatgagcgt caccgtcgcc ccacgaagcg ccgctcgcgc cgccaaggcg aagcccattt
     3541 tgccagaaga gcggttggtc agaaggcgga ctggatcgac ggcttcgacg gtgggccctg
     3601 ccgtgacgac caggtgctta ccgagccaag taggctcgag tagctcggcg acgactcgag
     3661 tcacgatatc ttccggttcg gagagccgcc cctcaccaat gtcccccgac gcaaccggac
     3721 ctgacaccgg cccaatgcgc tgcactcgcc cttgggcctc gagcgcctgc cagttccttt
     3781 gggtggccgg atgcttccac atgttggggt gcatggccgg ggcgaccaga accgggcaag
     3841 tcacacacag cgcgagcgcg ccaagcaaat catcggcgcg accggtgctc attcgggcca
     3901 ggaagtcagc ggtcgcgggc gcgatcaaga acaagtcgca gcctctggcc aggtcgacgt
     3961 gcagttcgcc gcctcgggaa ggatcgaaca tggcctgata cgcgggccta cccgtcaggc
     4021 ccgagaacgt tgcgtcgccc acgaactggg tcgccgcggc gctcagaacc acctccacgt
     4081 cggccccctc tttgaggagc agacgcaaca ccgcgaccgc cttgtatgcg gcgatgctgc
     4141 ccgtcacgcc aaggacgatg cgcttgccac gcaggcgcgg ctcgggcgcg ggtgagggga
     4201 acggctcggg gtagcgctcg agtggcgggc gacgcaagga gctcatgggg cgcgcactct
     4261 acacgcgatc gcgcgtggca cgagagaccg agacgaaggc gctctccgca tcgtaaaaac
     4321 ggtacggttt gagggcccat tctcccgcgt aatccacgcc gatccgcgcg ctgcgagaga
     4381 ctcgcggctt ggatcggcgc gtgagtgccg agccgtcggc ggccgtaccc tgggaggctc
     4441 cggcgtccaa ggcgctgccg tcgaaggtgc cgtcgtccag gtagagtggg cctgtctcca
     4501 agtcagcgcc gtaagccgcg cggtcgatgc cgaaggcctg gcagagcttg cccgggccat
     4561 tcgtcagcgc gcaacgctcc cgcatgctca ttcgttccca cggcttgctg gcaccgcgac
     4621 ggcgagcgat ctcggcgaag ccctcgagcg gttccaacgc acgcacgagc acggcttgcg
     4681 gttctcctgc gcggccagtc accaagttga aattccaatg catgccgtac acgaagaaca
     4741 cgtaaacgtg ccccggcggc ccgtacatca cctcggttcg cgccgtgcga cggccgccgt
     4801 aagagtgggc ggctcgatct tccggacccg cataggcttc ggtctctacg attcgtccgc
     4861 gcaggcgccc cgcgggcgtt tcgtgcacca agactttacc gatgcaatcg cgtgccacca
     4921 ccaacaccgg gcgcgcgtag aagtctctcg tcaaccgcac tcgaagccgc tcctatcctg
     4981 cccgcgccgc agaacggcga actgaagcgg gctaaaaacc cagcagccgc ccgccttgat
     5041 aaaccaggaa gctcgtagcg taggccagca acgtcatgta cccgaacatg aaagccggcc
     5101 agcgccagct gcgcgtttca cggcgcacca ccgcgagcgt gctcacacat tggcaggaaa
     5161 gtgcgaagaa caacatcaag gacaggccaa tcagcggggt atagcgcggt tttccgtccg
     5221 aggtgcgttc ctcgcgcatc ttctcgcgga ggggcttggc ttcgtcatcc agatcctcca
     5281 gcccataaac caagcccaag gtggaaacga agacctcccg tgcggcgaaa gcg
//
LOCUS       DASTKU010000243         7137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_717061, whole genome shotgun sequence.
ACCESSION   DASTKU010000243 DASTKU010000000
VERSION     DASTKU010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717061"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /locus_tag="VFQ61_08895"
     CDS             complement(<1..1686)
                     /locus_tag="VFQ61_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954609.1"
                     /translation="MDLEQRLKQLESYHDWTGVVEALEQGVLTSEDASKKADYHLRLG
                     RLLGQYFLQGVRALKHFQDAYKLNPAFVGALSEARGIYWELGKLNMVQKLIELQLKNT
                     QDPKFRGELLGELGDVVNDLGDYDRARDAYTKAGAEAAERLADVQVGAAEWQERIAAL
                     LRESQQTAGRVERGQLLLRAARIARRFAPTELEELLGKAYQADPANVNIAALFEGMLV
                     EGQRTNEILERQKEAAKAAQDPAERGLLLRAFGARWALRHQNPELAAELLEESLRADP
                     KSESAFTFIREYYGTKNGNWERVIRLADELSDRSGLGHDAGNLLALGGLIAWKERGDL
                     ILAKRFFQRLAQTEPQNPALSAFELQIGEKLSLSPGRGPSEAPRSNEVRRPVSEPPAA
                     LAPEPAPTPVAPPVEPAQITAEPPVVEAAPPVVSMPEPEPVRVVRAPSEPAPAAPPPV
                     VAPVSVAPASAGEDSRLVTELRERLHQQESAKRYHEYVKTLLALGDAVRDPSEREELY
                     AKAGDLYVNKFANQGEAVKAYEKVLEVNPGSATAVAYLREMYEKRRDWEKLIHL"
     gene            complement(2399..3310)
                     /locus_tag="VFQ61_08900"
     CDS             complement(2399..3310)
                     /locus_tag="VFQ61_08900"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9954610.1"
                     /translation="MNAWKLTTVAVLTLGLTAATGCSRDHIEAINLANEGDQSVKVNV
                     EGAIQKYEQAVQLDPTNHRILWKLAAAYEKKEDWDKMASTLSRAVQVAPDFANYWYKR
                     GEALNNQAEAGNPDAYEQSKEPLKKCIEKDPNIAECYYLLADAQLWTGDEQSAAEYYQ
                     KAIEHDPTWSFFYPPLAELYLTYRMVNEAETVLKEGTKLVPPSEKNNPKIYGMYVLLA
                     NVAQMKKDKAGQLDAMKNAERYAGDAHPELAFMLGSTYAVMSPPEKEKAVRLLDQFTK
                     KVCRGAASAKFKEECAQAAALVQKLGQ"
     gene            complement(3752..5818)
                     /locus_tag="VFQ61_08905"
     CDS             complement(3752..5818)
                     /locus_tag="VFQ61_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9954611.1"
                     /translation="MAKAVLTFAIYQYDALQRRETVTQDIVKVGKDPKSHLRVDDDLA
                     SRMHAVIEVAGPEDITLIDLGNEPGTMVNGARVNKCKLHVGDQIQVGNTVILLESAEP
                     AGVEAHPAAGLPQAPPSQRAAAPMPMQNPFAAAPSAPYAAPPAPFAAPPAPFAPPQSP
                     FAPAPNPFAAAAPVNPFGGHGHGHGVQDDMPPGTYTYTMLKSGPDVSPDEVELPYVQA
                     VEVMVLWGSNVLHVSHLTPPRNFYVGEEQGKTSTCDFFIPSERIGTTRLPVVVGDRMS
                     VAVVIPPAARGYVEVPGQGRMSLDEVRARAQPTAEVTGGHQFPLPAGAKARVELGDFV
                     FQVAAVNAGKPMKKGVATTFDWAVAAYFGLSFLVHAGFIAAMAFFVPPLGLTDDEDLD
                     KDRLYLMQQFLDAAAEREQQEKEAEVVEEQADNKEGGTGTRAKGEEGSMGNPTTKATN
                     KRYAVQGPKDNADPHIARQAALREAMEFGMIGLLNTGAAGDPNAPTAPWGRDTSLGTD
                     EVSARGNMWGDEIGDAFGAGGLGLSGIGEGGGGRGEGIGLGNIGTLGHGAGTGTGQGF
                     GAGHGRLGGSHSTRAPKVRMGATTVSGRLPPEVIQRIVRQNFGRFRMCYEQGLGRNPN
                     LEGRVSVRFVIGRDGAVSNVSNGGSDLPDSGVVSCVVSAFYGLSFPQPEGGIVTVVYP
                     IMLAPG"
     gene            complement(6046..6330)
                     /locus_tag="VFQ61_08910"
     CDS             complement(6046..6330)
                     /locus_tag="VFQ61_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954612.1"
                     /translation="MKSSAESKSKRIVLAAVVAGLTVLVASPAFAQDAADKGDKGEGY
                     GYEFEDDPLNAGGFGPNDATIKVRPRAARTTLIRPRTSFVTEMLKSVENI"
     gene            complement(6502..>7137)
                     /locus_tag="VFQ61_08915"
     CDS             complement(6502..>7137)
                     /locus_tag="VFQ61_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954613.1"
                     /translation="LSFRGYQEAEKAYREALKLRPKEYEAYLGLALAIRGQVNDGNFE
                     KAVSESQKYLDEAKKLDGSRAETYYNEAILTHEFRAKRAGDKAVPILRKAVEQFRDFV
                     SKAGDDPVFAAAVKRSNDRVEDIEATIKFLEEGEKARIEEEKAAKEAEEMKKAADAEA
                     KAKAEAEAKAKAAAPPPAAGAPPAAGAAPADPKAAGGAAAPKASEKPAPKK"
BASE COUNT         1218 a         2383 c         2145 g         1391 t
ORIGIN      
        1 cagatgaatg agcttctccc aatcgcggcg cttctcgtac atttcgcgca ggtaggccac
       61 ggccgtggcg ctacccgggt tcacctcgag caccttctcg taggctttga ccgcttcacc
      121 ttggttcgcg aacttgttga cgtagagatc gccagccttg gcgtagagct cttcgcgctc
      181 gctcggatcg cgtaccgcgt cgcccaatgc gagcagtgtc ttcacgtact cgtggtaacg
      241 cttggcgctc tcttgctggt gcaggcgctc gcgcaactcg gtcacgagtc gggagtcctc
      301 gccggcggac gcgggtgcaa cggatacggg tgcaacgacc ggaggggggg ccgcgggcgc
      361 gggttcggaa ggcgctcgaa ccacccgcac gggttctggc tccggcatgc tcaccaccgg
      421 tggcgcggcc tccacgacgg gcggctcggc cgtgatttga gcgggctcca ctggaggagc
      481 gaccggggtc ggtgcgggct cgggtgcgag cgctgccggc ggctcactca ccgggcggcg
      541 tacctcgttc gagcgcggag cctcgctggg tccacgccct ggcgagagac tgagcttttc
      601 cccgatctgc agctcgaatg cgctgagcgc ggggttctgc ggctcggtct gcgccaagcg
      661 ttggaagaag cgcttcgcga ggatcaagtc gccgcgctct ttccaagcga tcagcccgcc
      721 gagagcgagc aggttaccgg cgtcgtgacc tagacccgaa cgatccgaga gctcatccgc
      781 caggcggatc acgcgttccc agttaccgtt cttggtcccg taatactcac ggatgaaggt
      841 aaaggcggac tccgacttgg gatccgcgcg caacgactcc tcgagtagct ccgccgcgag
      901 ttccggattt tggtgccgaa gcgcccaacg tgcgccaaag gcgcgcagca acaacccgcg
      961 ctcagccggg tcttgcgcag ctttggccgc ttccttctgg cgctcgagaa tttcgttcgt
     1021 gcgctggcct tccaccagca tgccctcgaa caatgccgcg atgtttacgt tcgccgggtc
     1081 cgcttggtac gccttgccga gcagctcttc gagctccgtg ggggcgaagc ggcgcgcaat
     1141 acgcgcggcg cgcagcagca gctgcccgcg ctccacccgc cctgcggttt gctggctttc
     1201 acgcagcaga gccgcaatgc gctcctgcca ctctgccgcc ccgacctgta cgtctgcgag
     1261 ccgctcagcg gcttcggcgc cggccttcgt gtacgcatcg cgcgcgcgat cgtagtcgcc
     1321 gagatcgttc acgacgtcgc cgagctcgcc taacagctca ccgcgaaact tcgggtcctg
     1381 ggtgttcttc agctgcagct cgatcagctt ctgaaccatg ttcagcttac cgagctccca
     1441 gtagataccg cgtgcttcgc tgagcgcacc gacgaacgcc gggttcagct tgtatgcgtc
     1501 ctgaaagtgc ttcagggcac gcacgccctg caagaagtac tgccccaaca agcgcccgag
     1561 gcgcaggtgg tagtcagcct tcttgctcgc atcctcactg gtgagaacac cttgctcgag
     1621 cgcctccacc acgccggtcc agtcgtgata actctcaagc tgtttgagtc gctgctctag
     1681 atccatgctt cctcgggcaa gctaaaccaa cacgggggcg ttcacggtcg cggctacgcc
     1741 ccgaaatccg acgcttttac agcaaagccc ggtcggccgc tagctgcggg caccaccagg
     1801 cgaactttcc aggggcatcc ggagaaccct gcctagcctc atcaaagagc cagggctctt
     1861 cgaggagccg ggactgtcct acagaccgga accctcgcct cgatcgacag agttcatgca
     1921 ggcgggtctt gccagggccc tgtgtgcggc gaacggtcct gtccgcgcac aaccacgacc
     1981 ggtgggcgct cgcttgctcc cgcaacgtag gcgccgcgtg ccgctgaccc gcgttcgagt
     2041 aactggcatc gacacgctga cggcccatgt cttggttgcc gcccgtcgcc caattgcgac
     2101 ggcgcgtccg agcgccacgg gtccgcccac gacctgccca aacaccgccc gctcctctgt
     2161 cacccgccca agctcccttg gccccgtcgg aagcgtcgcc tctacgcgga gcatcacgct
     2221 ccgcccaccg cgccggcgcg cgcgcgaaca tgttggcgat gaggcccccc gcggccctcg
     2281 tgaacgccaa ctgctgctca cgcccagcgt gggtccactg caagcacccc gagctcaata
     2341 gccggatcgc gctgagagta gacccacact gctgaccttc cgacattcgt aaactcgatt
     2401 attggcccaa cttctggacc agagctgcgg cctgggcgca ctcctctttg aacttggcgg
     2461 acgccgcgcc tcggcaaacc ttcttcgtga attggtcgag gaggcgaacg gccttttcct
     2521 tttccggagg actcatcacc gcgtaggtgc tgcccaacat gaacgcgagc tcggggtgtg
     2581 cgtccccggc gtagcgctcg gcgttcttca tcgcgtcgag ctgaccggcc ttgtccttct
     2641 tcatctgcgc aacgttcgcg aggagcacgt acatgccgta gatcttcgga ttgttcttct
     2701 cgctgggggg cacgagcttg gtgccttcct tgagcactgt ctccgcctca ttgaccatgc
     2761 gataggtcaa gtagagctcc gccagcggcg ggtagaagaa ggaccaagtc gggtcgtgct
     2821 cgatcgcctt ctggtagtac tccgccgccg actgctcatc gcccgtccac agctgcgcgt
     2881 ccgccagcag gtagtagcac tccgcgatgt tggggtcttt ttcgatgcac ttcttgagcg
     2941 gttccttgga ttgctcgtag gcgtccgggt tccctgcttc agcctggttg ttgagggcct
     3001 ccccgcgctt gtaccagtag ttggcgaaat ccggagcgac ctgcaccgct cgagaaagag
     3061 tggacgccat cttgtcccag tcctccttct tctcgtacgc ggccgcgagc ttccaaagaa
     3121 tccggtggtt ggtcgggtcc agttgtacgg cttgttcgta cttctgaatc gcgccctcga
     3181 cgttgacctt gacggattga tccccttcgt tggccaagtt gatggcctcg atgtgatcgc
     3241 ggctgcagcc ggtcgcggcg gtcagcccca gggtgaggac ggcaacggtg gtgagcttcc
     3301 aggcattcat cgcaggcttt acctctctcg acacacgaac ggggaacctt cggcgcgctc
     3361 gactgttggg gtgctttgtg gtgcgcccgc gccctcttgc ccgcgcgcct tcagcgcagg
     3421 cttgttcagg cggagtctgg ttttcgggca ggccgtttgg agacccttcg aagcgagatt
     3481 ttccttcgat gtcgcgaagg ccactccaaa ggggccctga gcataggcgg acgcccacct
     3541 tgaagtaaag gcggaactcg acccgaaatg ggtgttcctg ccccgctcgt gacacccttc
     3601 tgctcgagct tcttctttcg agtgttcagg cgcagtccat gcccggccct tcacaggaag
     3661 aagacgcaca aaagcagtcg ggcccaaccg caagtgcgat tgggcccgag tttggcgcgc
     3721 ccgcaggcag cgcgcccgag tcgtcgaacg ctcagcctgg ggccaacatg atggggtaaa
     3781 ccaccgtcac gatgccgccc tccggctgag ggaagctcaa gccgtagaac gcagacacca
     3841 cacaactcac cacaccgctg tccggcaagt ccgagccacc gttcgacacg ttcgagacgg
     3901 cgccgtcacg accgatgacg aagcgcaccg agacgcgccc ttccaggttc gggtttcgac
     3961 ccagcccctg ctcgtaacac atcctgaaac gaccgaagtt ctggcgcacg atgcgctgaa
     4021 ttacctcggg cggcaggcga ccgctcacgg tggtggcgcc catacgaacc ttgggggccc
     4081 gcgtggagtg ggagccgccg agtcgaccgt gcccggcgcc gaagccctgg ccggtaccgg
     4141 tgccggcacc gtgtcccaaa gttccgatat taccgagacc gataccttcg ccgcgaccac
     4201 cgccgccttc accgatacca gaaaggccca ggccgccagc gccgaaggcg tcgccgatct
     4261 cgtcgcccca catgttaccg cgggcgctca cctcgtcggt accgagcgac gtatcgcgcc
     4321 cccacggtgc ggtcggcgcg ttcggatcgc ccgcggcacc ggtgttgagc agaccgatca
     4381 tgccgaactc catggcctca cgcaaagcgg cctggcgcgc aatatgcggg tctgcgttgt
     4441 ctttcgggcc ctgaacggca tagcgcttgt tggtggcctt ggtcgtgggg ttacccatcg
     4501 aaccttcttc acctttggcg cgcgtcccgg tgccgccttc cttgttgtca gcctgttcct
     4561 cgacgacttc ggcttccttc tcctgctgct cgcgttctgc cgcagcatcg aggaactgct
     4621 gcatcaggta cagccgatct ttgtcgagat cctcatcgtc ggtcaaaccg agcggaggaa
     4681 cgaagaatgc catagcggcg atgaagcccg cgtgcaccag gaaggagagg ccaaagtaag
     4741 cagccactgc ccaatcgaag gtggtggcta cacccttctt catcggcttg cctgcgttca
     4801 ccgcggcaac ctgaaacacg aagtcgccca gctcgacgcg agccttggca cccgcgggca
     4861 atgggaactg gtgcccgccc gtgacttcag ccgtgggttg agcccgcgca cggacctcgt
     4921 ccaacgacat acgaccttgg cccgggacct ctacgtagcc gcgcgccgcg ggcggaatca
     4981 cgaccgccac ggacatgcga tcgcccacca cgacgggcaa gcgcgtggtc ccgatccgct
     5041 cgctcgggat gaagaagtcg caagtggagg tcttgccctg ctcctcaccg acatagaagt
     5101 tacgcggcgg cgtcaggtgc gaaacgtgaa gcacgttgga gccccacagc accatcacct
     5161 cgacggcctg aacgtacggc agctccacct cgtcggggct gacgtccggg ccgctcttca
     5221 gcatcgtgta cgtgtacgtg ccgggcggca tatcgtcctg aacgccgtgc ccgtgcccgt
     5281 gaccaccgaa tggattcacc ggagcagccg cggcgaacgg attcggagcc ggcgcaaacg
     5341 gcgactgagg aggagcaaaa ggagcgggtg gcgcagcgaa cggagccggc ggcgcggcgt
     5401 agggcgcgga gggcgccgcc gcgaagggat tctgcattgg catcggcgcg gcagcgcgct
     5461 gcgagggggg ggcttgcggc aggccggcgg caggatgcgc ctccacgcct gctggctcag
     5521 cgctctcgag cagaatgacg gtattgccaa cctgaatctg gtcgccaacg tgcagcttgc
     5581 acttgttgac gcgggcgccg ttcaccatcg ttcccggttc atttccgaga tcgatcagcg
     5641 tgatgtcttc gggcccggcg acctcgatca ccgcgtgcat ccgcgacgcc aaatcgtcgt
     5701 cgacgcgcag gtggctcttc ggatctttac ctaccttgac gatgtcctgc gtcacggtct
     5761 cgcgccgctg gagcgcatca tactgataga tcgcgaacgt caatacagcc tttgccatgc
     5821 gatctttcct agttcgacct aacgcagttc aacggacgac ttgcaaccca acagctaacc
     5881 aatcggatcc tctcctggcc tgaaacccgc caggagagcc gcgagttgcc cgcgccagcc
     5941 gaaggtaaag ggcgtgcggc gcgggactcg acagcttcat ctacgattca aactcgaggt
     6001 aattccaaat cgggacagtt ccgaatccta accgggcacc cgccgtcaga tgttttcgac
     6061 ggacttgagc atctcggtca cgaacgaagt acgcggacga atcaaggtgg tacgcgccgc
     6121 gcgcggacgg accttgatcg tggcatcgtt cgggccgaac ccgcctgcat tcagcggatc
     6181 gtcctcgaac tcgtagccgt agccttcgcc cttgtcaccc ttgtccgccg catcttgagc
     6241 gaacgccgga ctcgctacca acaccgtcaa gccagcgacc accgcggcta agacgattct
     6301 tttggatttg ctttcagctg acgacttcat tgcagtctcc agagaattct taactggaat
     6361 tacagtttta ttgtaacgac caactttgaa ctcgtcaacc ttgatttggc gacgagcagc
     6421 ctgacaacga agtcgagcca acgttcccga gcaggtcgag aaatatcgac acagctcgcg
     6481 gatcgttggc tcgagttccg ttcacttctt aggcgcaggt ttctctgaag ccttgggtgc
     6541 tgcagctcca ccagcagctt ttggatccgc gggtgcggcg cctgccgcag gaggagcacc
     6601 tgcggccgga ggcggagcgg ctgccttcgc tttggcctct gcctccgcct tggctttggc
     6661 ttccgcgtca gcggccttct tcatttcttc ggcttccttg gccgccttct cttcctcgat
     6721 gcgagccttc tcgccctctt cgaggaactt gatcgtggcc tcgatgtctt cgacgcggtc
     6781 gttcgagcgc ttcaccgctg cggcgaagac tggatcatcg cccgccttcg aaacgaagtc
     6841 ccggaactgc tccactgcct tgcgcagaat cggaaccgcc ttgtcccctg cccgcttggc
     6901 acggaactcg tgggtcaaga tcgcctcgtt gtaatacgtc tctgcgcgag acccatcgag
     6961 cttcttggcc tcgtccaagt acttctgcga ctcggagacc gccttttcga aattgccgtc
     7021 gttgacctgc ccgcggattg cgagggccag gccgaggtag gcttcgtact ccttgggacg
     7081 gagcttgagc gcctcacgat aagccttctc cgcttcctgg taaccgcgga aagacag
//
LOCUS       DASTKU010000244         8473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1833459, whole genome shotgun sequence.
ACCESSION   DASTKU010000244 DASTKU010000000
VERSION     DASTKU010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833459"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..2370
                     /gene="priA"
                     /locus_tag="VFQ61_08920"
     CDS             109..2370
                     /gene="priA"
                     /locus_tag="VFQ61_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET9954614.1"
                     /translation="MLLARVAVPVPLGQAFTYSVSAELSTQVQRGARVFCEFGRRRVL
                     AVVLDVGEREPDVPLDKLKPLVSVVDHEPVLPLELLEFLEELAKYYVAPIGEVLQLAL
                     PALERTQARALGESIKAKVVGRLVQFGRALPLPDSSAVVEGVVGAPKPERPLSARALE
                     LHAYLMEHGPTPITELQQNWKSARSMLKNSSAFAIEQRELEPSHAFDRPVVRDTAPEL
                     NVAQRAALTAIHAKLEAQERAAFLLDGVTASGKTEVYLGSVARALELGRGAIVLVPEI
                     ALTPQLVGRFRARLGDAIAVLHSALGEGERHAMWKALRAGTLRVAVGARSALFAPVHD
                     LGLICVDEEHDGSFKQEDGVRYNARDMALLRAHRARAVCVLGSATPSISSLALVERGR
                     LEHLVLPSRAHRAAQMPDVEIVDLRRIGAAPFGERLLSIRLFRELERTLEEKRQAILF
                     LNRRGFAPSLICDACGTITECPHCAVALTVHRARGERLRCHYCDYSSTRPSRCAKCGS
                     EHLSEEGAGTERIEAVLASALPGARVARLDRDVASGEKSAQIMDRMRQGEIDILVGTQ
                     MVTKGHDLPNVTLVCVLNADSALSLPDYRASERSFHLFVQVAGRAGRADAKGLVLIQT
                     RNPEHPAIRCALQHDVKSFVAQELALREELEYPPFSRLVLLRVDAIDAARAQQAAIQV
                     AKIARNSAPPEVRVVGPAPAPIPRLRGRYRFHVLLRAKERGKLQKPLIHVARAELNHA
                     VRLAIDVDPVNML"
     gene            complement(2488..3501)
                     /locus_tag="VFQ61_08925"
     CDS             complement(2488..3501)
                     /locus_tag="VFQ61_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954615.1"
                     /translation="MQTSETQADERWYVQYDSGEVRLMTLEEIDQEFEAGKIHENTYL
                     VQVGESKWQTLAEVAGLNEEEEAEAEPAPAPVVTPAVAAAPIPMPTQPAPAIAIAAPV
                     AAPISARPLPAQPAPVAPAMPSAQAAQAIPPYAQTVGQVAPAASFPPQAMPASFPPQA
                     MPASVRPQAAAPVLNYPSTPPVVQDLDLDLDVGARSFKSSSRRAAVIAAVAGLALIGG
                     GGYALARMDAPPEQPPVAASRPVAPPPSKPLLEDSLPPPSTPSTTSANSDSGNSAASS
                     KLSEDMKKALLAQDDDRAKKKGAKSGKASGKTVASKGSGKSSKGGGLKAGGDDHDPLN
                     SKL"
     gene            4156..4623
                     /locus_tag="VFQ61_08930"
     CDS             4156..4623
                     /locus_tag="VFQ61_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954616.1"
                     /translation="MPSEALAFERQWHVGLGVGLLQPKHADGLSLGVIGHAAYGLTDE
                     FDARFTLGATQTRGTAPESRATWFSFSTLGLSYKLDIIEWVPYLGVRAGFFRFEHALP
                     TYAKQGGSIGGMAGLDYSITRNTALGAEVGYDALLPQGALFTAVVRAEYRWGF"
     gene            4842..7046
                     /locus_tag="VFQ61_08935"
     CDS             4842..7046
                     /locus_tag="VFQ61_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET9954617.1"
                     /translation="MNSSQTAPNSGDAGPSLPTLEPLQPSQSQTSPPEYAQPEPPWAD
                     TLRAENWAEGFRVLAQEASKPESPPELRLAAAMCARNTGDYAASHALLDGLDAKLPEI
                     SARIRAHRDEAAYWAADRQSALSSCEGRVDPESRLKAAHLYRDLGYRNKALTQVDNLL
                     RGSFKRSELCTIEAPAHALRAELLPPTDLAGRAKELSWLALKATLCPAAEQAASQLQA
                     LPESYRLTKQQQLERAQAFANAGRVALAEDALASAALARGTILEPGVVEYVKGYARYQ
                     ARSDLALGAELLNRAAALNANHRPEWLFYSARALERAQDTVRARALYARVQKFPNSSF
                     ADHAEYRTAQILYSESQFAAAAQAYDRYLANRGRRARFEKDARDERAIAWLASGKPGL
                     AAREFREFSRTAREARERARYTELEGLALSRMGAAEAAKQRWLDVVHSYPLSFAAATA
                     AARLASQGAAVAPPEAPSSTGSEAATPRLPVEVALLHRIGLDREAEQALALLEPGLRR
                     NFAARSDEALCALYGRLAPAARRFRIGQGAAQTDELNRLPSNDRRWLWECVYPRPYGS
                     LIQQVADEHSLEPDLLYAIMRQESSFRPDVTSPAQAVGLLQLMPATAARLAQELGIPQ
                     ADLDLKQPYLNVRLGARYVRKLLDAMSGNIPLAVAAYNAGPAAVARWLDGAGDLGLDV
                     FVARIPYEETRTYVERVVSNLSRYRYLAGLSPRADADLLSLPKLSPEARALY"
     gene            7060..7998
                     /locus_tag="VFQ61_08940"
     CDS             7060..7998
                     /locus_tag="VFQ61_08940"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9954618.1"
                     /translation="MSTLSSSKTQRLAELVADKRRRGIIHPQAAFEHFTLDRYPPGSK
                     VAYFVEGYWRVSWDLQGRPPFVQETVSHPFTNLVFEKGGGRLYGVATRRFSRRLVHRG
                     QVIAVKFRMGGLYPFLRVSLARFTDLSLPARALFGEQFARLDEQFDAQTEPHSFLQRV
                     EEFICQRLPGRDPNVDLVNRIFERILSDSEVYRVDTIADHFRMNKRALQRLFQRYVGV
                     GPKWVIQRHRLQEALSALDHSPQLNLTALSHRLGYFDQSHFTRDFKALVGISPERYRQ
                     RSLRASLPVGDSNTAVVATIGVPHDQPSRDATLDRD"
BASE COUNT         1483 a         2789 c         2612 g         1589 t
ORIGIN      
        1 tacaccgagc tcgccgtacg caccctgcac gagggcttcg gcctggatcg cagcggcgtc
       61 accgagagct gaggtcggcg gctgtccgtc gaggttgcca gccagaagat gttgctggcg
      121 cgcgtagccg tacccgttcc gttaggccaa gcctttacgt actccgtgag cgccgagctc
      181 agcacgcaag tgcagcgcgg cgctcgcgtg ttttgtgagt ttggtcggcg ccgagtattg
      241 gcggtggtgt tggacgtggg cgagcgcgag cccgatgtgc cgctcgacaa gctcaagcca
      301 ctggtctccg tcgtcgatca cgagccggta ctcccgctcg aactgctgga gtttttggag
      361 gagctcgcga agtactacgt ggctccgatc ggggaagtgc tgcagctcgc gttaccagca
      421 ctcgaaagaa cccaagcccg tgcgctcggc gagtcgatca aggccaaagt cgtgggccgg
      481 ctcgtgcagt tcgggcgagc actgcccttg cccgactcct cggcggtcgt cgaaggtgtg
      541 gtcggcgccc cgaagcccga gcgcccgctc tcggcgcgcg ccctcgaact ccacgcctac
      601 ctgatggagc acggccccac tccgatcacg gagctccaac agaattggaa gagcgcgcgg
      661 tcgatgctga agaacagctc cgcattcgcg atcgagcaac gagagctcga accgagtcac
      721 gcattcgatc gcccggtcgt gcgtgacacc gcgccggagt tgaacgtcgc gcagcgcgcc
      781 gctttgactg cgattcacgc caagctcgaa gcgcaagaac gcgcggcatt cctactcgat
      841 ggcgtaaccg catccggcaa aaccgaggtc tacttgggat ccgtggcacg cgcactcgag
      901 ctcgggcggg gcgcgatcgt gttggtgccc gagattgcgc tcacgccgca gttggtcggg
      961 cgatttcggg cccgcttggg cgatgccatt gccgttctcc acagcgcgct cggtgaaggc
     1021 gagcgccacg cgatgtggaa agccctgcgc gcgggcacac tgcgcgtcgc ggttggcgcg
     1081 cgctccgctc tgtttgcccc cgtccacgac ctcgggctga tctgcgtcga cgaggaacac
     1141 gacggctcgt tcaagcagga agacggcgtt cgctacaacg cccgcgatat ggcgctgctt
     1201 cgggcgcatc gggcgcgcgc cgtgtgcgtg ctcggctccg cgactccgtc catcagcagc
     1261 ttggccttgg tcgaacgtgg gcgcttggaa cacctggtgc tgccatcgcg cgcgcatcgc
     1321 gcggcccaaa tgcccgacgt cgagatcgtc gatttgcgcc gcatcggcgc cgcgccgttc
     1381 ggggagcggt tgctcagcat tcgacttttc cgcgagctcg aacgcacgct cgaagagaag
     1441 cgccaggcaa tcttgttcct gaaccgccgc ggctttgcgc caagcttgat ctgcgatgcg
     1501 tgcggcacca tcaccgagtg cccgcactgc gcagtggcgc tcaccgtgca ccgcgcacgt
     1561 ggtgagcgcc tgcggtgtca ctactgcgac tactcctcca cgcgcccgag tcgttgcgcc
     1621 aagtgtggca gcgagcactt gagcgaagag ggcgccggca ccgagcgcat cgaggccgta
     1681 ttggccagcg cgctacccgg agcgcgcgtg gcgcgcctcg atcgggacgt cgcctcgggc
     1741 gagaagagcg cccaaatcat ggatcgtatg cgccagggtg agatcgacat tctggtgggc
     1801 actcaaatgg tcaccaaggg tcatgatttg ccgaacgtga ctttagtctg cgtattgaac
     1861 gccgactcgg cgctgtcttt accggattac cgcgcttcgg agcgcagctt ccatttgttc
     1921 gtgcaagtgg cggggcgcgc ggggcgcgcc gatgccaagg gcctagtcct gattcaaact
     1981 cggaatccgg agcaccctgc cattcgctgc gccctccaac acgacgtcaa atcattcgtg
     2041 gctcaggagc tggcgctgcg ggaagagctc gaatatcccc ccttctcgcg cttggtgttg
     2101 ctccgggtcg acgccatcga cgccgcgcgg gctcagcaag cagccatcca agtcgccaag
     2161 atcgcccgca attcggcgcc tcccgaggtg cgcgtcgtcg ggcccgcacc cgcaccaatt
     2221 ccccggctac gtggtcgtta tcgctttcac gttttgttgc gcgccaagga gcggggcaag
     2281 ctccaaaagc cgctcattca cgtagcgcgc gcggaactga atcacgccgt gcggctcgcg
     2341 atcgacgtcg atcccgtcaa catgctctga acgggagttc gtttcccgcg ttctcgaagc
     2401 cgctcgctcg acgagcgaaa cgggattgtt cggagcagag agcacgctct cgaacgatcc
     2461 cgttttccca ctgcgcgctc gaaaaactca gagcttggag ttcagcgggt cgtgatcgtc
     2521 gccgccagcc ttcaagccgc cacctttgga gctcttcccg ctacccttgg aagcgacggt
     2581 cttgcctgac gccttgccgc tcttggcgcc tttcttcttc gcgcgatcat cgtcttgagc
     2641 gagcagcgcc tttttcatgt cctcactcag cttgctgctc gcggcgctgt tccccgagtc
     2701 cgagttggcg ctcgtcgtag aaggagtcga cggcggcggc agtgaatcct cgagcagcgg
     2761 cttcgaggga ggcggcgcaa cgggacgaga cgccgccact ggcggttgct ctggcggagc
     2821 atccatgcgc gcgagtgcat agccaccgcc accaatcaga gccagccccg ccaccgccgc
     2881 aatcaccgcg gctcgacgcg agctcgattt aaaggagcgg gccccgacgt ccaagtccaa
     2941 atccaagtcc tgaacgacgg gcggagtgct cgggtaattg agcacaggcg ctgccgcctg
     3001 cggccgcacc gaggcaggca tcgcttgcgg tggaaacgag gcgggcatcg cctgcggtgg
     3061 aaacgaagcg gccggcgcga cttgcccgac ggtctgagcg tagggcggga tggcctgcgc
     3121 ggcttgcgcc gagggcatcg ctggcgcgac aggcgcgggc tgagccggga gcggacgagc
     3181 cgaaattggt gcggcgacgg gcgcagcgat cgctattgcc ggcgccggct gagtcggcat
     3241 aggaatcgga gccgcggcaa ccgcaggggt tacgacgggc gcgggagcag gctcggcctc
     3301 cgcctcttcc tcctcattca aacccgccac ttccgccagg gtttgccact tcgactcgcc
     3361 tacctgaacc agataggtgt tctcgtggat cttccccgct tcgaactcct gatcgatttc
     3421 ttcgagcgtc atcaggcgaa cttcgcctga gtcgtactgc acgtaccaac gctcgtccgc
     3481 ttgagtctcc gaggtctgca taaggcatct ccgactgcgg gggttgagcc accgcggtgc
     3541 tgcagtgaag ttaactcaga atcaggcaaa tgctttggac attcgcaacc aactctgcgc
     3601 agcatttgaa cacgcaacgg aacattcgca cccgaactgc aaagagctga acttcgcttc
     3661 agcttcgcac gcaccaagca accgggctcg aaaagcgcca aaattctggc ccggtttcgg
     3721 atgggggctg cactagcctc gtcactcacg acctgcaacc ctgcggcgag tacctgtgag
     3781 ctgcgtgctg agccccatca aatcgcctgc aattttgcgc acgaactggg cgttttctgg
     3841 gggacaccgc gtcccagcgc tgggggacac taccattgca gcagtgaacc gagggcacgg
     3901 ctcgaggggt cgagcaagcg ccggctcgag actggctcgc gatccgtggc gtcgattacc
     3961 cgcctctatc gaggcacggt cgaggcacgt gtgctcgaaa atccgcattc ttcgcgattt
     4021 tttggccgac actcaaggtt tttacgtgta cgccaagacc gtcactttct tctcgtcatg
     4081 cgcctccaaa gaactcgtct ccgctgcttc gcactcgcgt tcacctcgtc ttgttcgctt
     4141 ttttgcctac ttggcatgcc ctccgaagcc ttggcgttcg agcgccaatg gcacgtcggt
     4201 ctgggtgtcg ggctactgca gccgaaacac gcggatgggc tgagcttggg cgtgattggg
     4261 cacgccgcgt acggcttgac tgacgagttc gacgcgcgct tcacgctcgg agcgacgcag
     4321 acgcgcggca ccgctccgga gtcacgcgcg acttggtttt cattcagcac actagggctc
     4381 agctacaaac tcgacatcat cgagtgggtc ccttatctcg gcgtccgcgc aggattcttt
     4441 cggttcgagc acgccctacc cacctacgcc aagcaaggcg gctcgatcgg aggcatggcg
     4501 ggcctggact actcaatcac ccgtaacacg gcactaggtg cggaggtcgg gtatgacgcg
     4561 ctgctgccgc agggagcgct gttcacggcc gtagtgcgcg ccgaataccg ctggggtttt
     4621 tagatcagcc aaaaaacgcc actttctcgg caggccccgc gccatatgat acccctcctc
     4681 gagtgcgggc acgtcacgcc atcctaatta cgtgttggtg cgcttgggct tgcgcgggcc
     4741 cggccgcacg cgagcggagt caccccgaaa ccgcgctgag cgtgccgacg cgtgctagcc
     4801 agccgggagc ttcgaactgg ggcattcttg gcaaggccgc cttgaactcg agtcaaaccg
     4861 cgccgaactc gggcgacgcg ggtccgagcc tccccactct cgagccgctc caacctagtc
     4921 agagtcagac tagcccgccc gagtatgccc agcccgagcc cccgtgggcg gacactctgc
     4981 gtgcggagaa ttgggccgag ggtttccgag tactcgcgca ggaagccagc aaacccgaga
     5041 gtcctcctga attgcgactc gcggccgcaa tgtgcgcgcg taatacgggt gactatgcgg
     5101 cgagtcacgc gttattggac ggcctcgacg caaagttgcc ggagatctcg gcgcggattc
     5161 gggctcaccg ggacgaggcc gcttattggg ctgccgaccg ccaaagcgcg ctctcgtcgt
     5221 gcgagggccg agtcgatcct gagagtcgcc tgaaagcggc gcacctgtat cgcgacttgg
     5281 gctaccggaa caaagccctg actcaagtcg ataacctact gcgcggctca ttcaaacgct
     5341 ccgaactctg cacgatcgaa gcccccgctc acgccttgcg cgccgagttg ctgcccccga
     5401 ccgatctggc cgggcgcgcc aaagagctga gttggctcgc gctcaaagca acactctgcc
     5461 ctgccgccga gcaggccgcc agccagttgc aggcactgcc cgaatcgtat cgcctgacca
     5521 agcaacaaca gctcgaacgc gcccaagctt tcgcaaacgc agggcgcgtg gccctcgcgg
     5581 aagacgcgct cgcatcggcg gcgcttgccc gaggcacaat cctcgagcct ggcgtcgtcg
     5641 agtacgtcaa aggctacgcg cgttatcaag cccgcagcga tctcgcctta ggtgcggagc
     5701 tcttgaatcg cgcggccgca ctgaatgcca atcaccgccc cgagtggcta ttctattcgg
     5761 ctcgcgcact ggagcgcgcg caagacacgg tccgcgcccg cgccttgtac gcgcgcgtgc
     5821 agaaattccc gaacagcagc ttcgcagacc acgccgagta ccgaaccgcg caaatcttgt
     5881 attccgagag tcagtttgcc gcggccgcgc aagcctacga tcgatatttg gccaaccgag
     5941 gccgtcgcgc tcgcttcgaa aaagacgcgc gagacgagcg cgcgatagcg tggctcgcga
     6001 gcggcaagcc gggcttggcc gcgcgtgaat ttcgagagtt ctcgcgtacg gcgcgcgaag
     6061 cccgtgagcg cgcccgctac accgagctcg agggcttggc cttatcgcgc atgggtgcgg
     6121 ccgaagctgc gaagcagcgc tggctcgatg tcgtgcatag ctatccattg tccttcgcag
     6181 cagcaaccgc ggcggctcgt ctggcgtctc agggggcggc cgttgctccg ccggaagctc
     6241 cgtcttctac gggctcggag gcggccactc cgcgattgcc agttgaggtc gcgctgctgc
     6301 atcgaatcgg gttggatcgc gaggccgagc aggccctggc cttgctggaa ccggggttgc
     6361 gccggaactt cgcggcgcgt agcgacgagg cgctctgtgc cttgtacggt cgcctcgcgc
     6421 ccgcggctcg ccgcttccgg attgggcaag gagcggctca gacggacgag ttgaatcgtt
     6481 tgccgagcaa tgaccggcgt tggttgtggg aatgtgtcta cccgcgtccc tacggatcgt
     6541 tgatccaaca ggtggctgac gagcacagtc tcgagcctga cttgctttac gctattatgc
     6601 ggcaagagag ctcctttcgg cccgacgtga cctccccggc gcaggccgtg ggtctgctgc
     6661 agctgatgcc cgctacggcg gcgcggttgg cgcaggaact cggaattccc caggctgacc
     6721 tcgatctcaa gcagccgtat ctgaacgtgc gtttgggcgc gcggtacgta cgtaaactct
     6781 tggacgccat gtccggcaat attccgctcg ccgtggccgc gtacaatgct ggtcccgcgg
     6841 cggttgcacg ctggctcgac ggcgcgggcg acttaggcct agacgtgttc gtcgcgcgta
     6901 tcccctacga ggagacccga acttacgtcg agcgggtggt atccaatctt tctcgatatc
     6961 ggtatttggc cggcctgagc ccgcgcgcgg atgccgacct cttgagtttg ccgaagctct
     7021 ccccagaggc gcgcgccttg tactagaacg cgagtcgtct tgagcacgct gtcctcgtcg
     7081 aaaactcaac gtcttgcgga gctcgtcgct gacaagcggc gtcgcggaat aatccacccc
     7141 caagcggcat tcgaacactt taccctggat cgttacccac ccgggtctaa ggttgcttac
     7201 ttcgtcgagg gctattggcg cgtctcctgg gacttgcagg gccgaccgcc gttcgtgcaa
     7261 gaaaccgtat cccatccgtt cacgaacttg gtgttcgaga agggtggcgg gcggctctac
     7321 ggggtcgcca cacgccgatt ctctcgtcgg ctcgtgcatc gtggccaagt cattgccgtc
     7381 aagttcagga tgggtggtct ctatccgttc ctgcgggtca gcctagcccg gttcaccgac
     7441 ctgtctctgc ccgcgcgggc cctcttcggc gaacaattcg cccgtttgga cgagcaattc
     7501 gacgcccaga cggaaccgca ctctttcctc cagcgcgtcg aagaattcat atgtcagcgc
     7561 ctaccgggtc gagatcccaa cgtggacctc gtgaaccgga tcttcgagcg catcttgtcc
     7621 gacagcgaag tctatcgagt ggacaccatt gccgaccact ttcgaatgaa caagcgcgcc
     7681 ctacaaaggc ttttccagcg ctacgtcggg gttggtccca agtgggtcat tcagcgtcat
     7741 cgcctccagg aagccctcag cgccctcgat cacagcccac agctcaatct gactgcgctc
     7801 tcacatcgac tcggatactt cgaccaatcg cacttcaccc gcgatttcaa agcattggtc
     7861 ggcatcagcc ccgagcgtta ccgtcagcgt agcctgcgag cgtcacttcc tgtcggcgat
     7921 tcgaacactg ccgtagtcgc gaccatcggc gtgccgcacg accagccctc ccgagacgca
     7981 accctcgatc gtgattaagc cccggtgcac ggctcgatga tgggcggcac acaaggttac
     8041 gagcaagtcc ggatcgtgta ctccgccttc cgaccgtgga atgagatggt gcacatccaa
     8101 aaaagtagag caccggcacc ccggaacgat acaccttccc ccgtctcgtc ggagaacttg
     8161 cctccttacg cggggcgctg ggtcttgcgt cgcccgcgtg ggcgcggccc gttcgcgtgc
     8221 cggttctaca tcgccaatac tctttccatc gccagattgc gcggacgact ccagaggcgt
     8281 ggggcgcgga caacccgtgg tcgtcgactt agaaacatgg ctcgctttta cccccgcact
     8341 caccaccggt gcgccgccca cgtcgccaat atgttgcgca tcacacgacg ccatgtcgag
     8401 tacatcacca ctgattggga ttcgcgcccc cgccgcatcc tgaaatgcct gctggcaccg
     8461 ttcacaaact gtg
//
LOCUS       DASTKU010000245        29417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_49524,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000245 DASTKU010000000
VERSION     DASTKU010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 29417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29417
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49524"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFQ61_08945"
     CDS             complement(<1..199)
                     /locus_tag="VFQ61_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954619.1"
                     /translation="MQPISPNAAITPSILNRQCCDGGRAPSMLHVCEARSAIQVSGSI
                     LVRRKYPSHETESPAPGPHVGK"
     gene            complement(2189..3640)
                     /locus_tag="VFQ61_08950"
     CDS             complement(2189..3640)
                     /locus_tag="VFQ61_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954620.1"
                     /translation="MPRQKKLSAERLDTHQKALAINLDARRYGTFAEIGAGQEVVRWF
                     FRVGGAAGTIAKSISAYDMAVSDAIYGSAQRYVCRQRLESMLNYEHRLNLERLREARG
                     DATAFFAFADTVAAKSYRGTNECHGWLGVRFQAHPRDEDSQIIIHVRMLDSENALQQE
                     ALGVVGVNLLHGAFFMHHDPDRLVDSLLDNLSTDRIEIDMIEFSGIEFRHVDNRVMAL
                     RLVELGLSGAAMFDPEGHVLQPSEVLYKRPVLVERGAFRPVTRTNLDMLRTARDEFTR
                     AAGGDPEPILELSEITMRNLIESGQGDIDYRDFLARADMLAAAGKAVLISDYFEYYRL
                     VGYLRRYTQKPVAIVLGAGSLSPLFDERYYLDLPGGILESFGRLFKNDTKLYVYPFLD
                     PKQPDLLTAANFEPAPELRKLYGYLIDRGCIVPLEGYDPECLRIYAADILRLIQSGDP
                     NWENLVPPEVARVIQKRALLGYRRDSALRRPPS"
     gene            3848..7177
                     /gene="treS"
                     /locus_tag="VFQ61_08955"
     CDS             3848..7177
                     /gene="treS"
                     /locus_tag="VFQ61_08955"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HET9954621.1"
                     /translation="MSSAEADALWYKDAIIYQLHVRSFSDSTNDGVGDFKGLADRLDY
                     IRDLGVTAIWLLPFYPSPLKDDGYDIADYTNVNPSYGTLADFEHFLREAHDRKLKVIT
                     ELVINHTSDQHPWFKRARRAPKGSVERDFYVWSDTPDLYREARIIFKDFEHSNWTWDP
                     VAGAYFWHRFYSHQPDLNFQNPEVRKAVLKAMDFWFALGVDGFRLDAVPYLFEREGTS
                     CENLLETHLELKHLRSHVDNHFKHRMLLAEANQWPEDAAAYFGSGDECHMAFHFPLMP
                     RMFMALKMEDRYPVLDILNQTPAIPDNCQWAVFLRNHDELTLEMVTDEERDYMYRTFA
                     KDPQARINLGIRRRLAPLLGNDRRQIELMNGLLLAMPGTPVIYYGDEIGMGDNIFLGD
                     RNGVRTPMQWSADRNAGFSRASSQRLFLPVITEAGYSYEALNVEAQHGNSLSLLWWMR
                     NLISLRRRHRAFGRGTLEFLYPANRKVLAFLRCHEGEELLVVANLSRFAQCVELDLSS
                     RAGKIPVEMFGHTRFPAVANTPYTLSLAPYGFYWFTLAPPKLSLDEGAPRDETKDLPN
                     FYVEETWLDLLRGPSKPQFEEFVSSLLPSRRWYAGKARTIKTAELIEVIPLAENNSCL
                     ALFRVDYLEGDPETYLLSMTYARGERAREVAQDPGSAVARVWVDGEEGILFSGVRDMK
                     LNKTLLDLLSTGQKLEGRRGNLFAITGPSGAARLADLIAKNSPLEPNLLSAEQSNTSI
                     AFGKSLILKLFRKVEEGRNPDVEIGEFLTEEAHFPHTAPLLGYLEYRPSEGEPSTLAV
                     LQAFVPNDGDAWNYTLDHVRHYFERTLSRPSDSILPAAPGPILTLASGTIPGVVHELV
                     GGYLESARLLGQRTAEMHLALLTPTEDPQFKPEPFTPFYQRSLLQSFRNLTEEVFSRL
                     GSRLETLAPEVQGRARQVLSLKSRIGDVFRGIVSRNVSALRTRLHGDYHLGQVLWTGR
                     DFIIIDFEGEPLRPISARRIKRSPLRDTAAMIRSLNYAAHQGVHNLRRQGLPVNTNLD
                     LDDLAEHWYQWSASAFLRAYLRTAEGAAFLPKDREELALLLDVYLLEKAVYELDYELR
                     HRPNWVHLPLRGIERLVARSG"
     gene            7349..8083
                     /locus_tag="VFQ61_08960"
     CDS             7349..8083
                     /locus_tag="VFQ61_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET9954622.1"
                     /translation="MPSVVCIGTGEAFDPALPNNSLFYRGSINLLIDCGFAVPQAFFS
                     RVRDPSLLDAIYITHHHADHAFGLPAVLLWMKEEGRTRPLELIGGPGLAEYAEKLMTL
                     AYPGSFEDCYPIIPVELTPGRVLKRGSAELRNARSSHRVRNLALRIDDLGRSFCHSGD
                     GAPTPETLSLYRRANLLSHECYFAEQETESHAHFAEVLALAEVAHVETLMLTHLGRKE
                     KERIHQLAMSTPRKVIIPKPGAHIHI"
     gene            complement(8115..10328)
                     /locus_tag="VFQ61_08965"
     CDS             complement(8115..10328)
                     /locus_tag="VFQ61_08965"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HET9954623.1"
                     /translation="MEVQKIDRRVILVSNRLPVAVKLDGDRLEVEPSSGGLATGLQGF
                     RASGGRTLWIGWPGAAARSDEQRFLMDQRLAELDAVPVHLSSHESQRFYEGVANGVLW
                     PLFHYLIDRVPLVIRHWDAYVQANRRFCDVVVSHARDGDVIWIHDYQLMLLPAMLRAR
                     LPHATIGFFLHIPFPASEVFRTLPQRDMVLHGLLGADLIGFHSYDYLRHFSDSLLGLL
                     GLEVELDHLQVDGREVRLGVFPMGIDATTFAERAAADEVLAEMQTLRAAHGVERLLLG
                     VDRLDYTKGIPRKVLAMERLLERTPALRGRVRLLQVAVPSREKVDAYKSLRRDVEELV
                     GRVNGAHGTLAGAPVHYLYQSVTGNQLAASYRAADVMVVTPLRDGMNLVAKEFIASRV
                     DGDGVLVLSELAGAASELGEALTVNPYDVEALAASFERALSMPESERRHRMAVLRQKV
                     LARPVQVWAESYLSALEVRPADALQRDAPLDDVEVDELAGELALEPEVVLAIDYDGTL
                     VPLRARPELAFPDGELLELLGELARAPRTSVYVVSGRTRESMEAWLGQLPIGMIAEHG
                     VFVRRLGEAEFHAQFDFSGSGFRSMVRAIFEEYMVRTPGAMIEEKSAGLAWHYRNAAP
                     HLGAVRSRELRMHLVQTLAQSPVNILTGHKVVEVRPQGIDKGQSLRSVLAELPGARAL
                     AAFGDDRTDEDLFASIPDARFTFCAGPLPTRARYRLDGPRQVRAFLRRYIQRRTR"
     gene            10677..14024
                     /locus_tag="VFQ61_08970"
     CDS             10677..14024
                     /locus_tag="VFQ61_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9954624.1"
                     /translation="MSKRWLTQTRLEHGAGALTLEGAGPKGTAAFEVFRRSFDGSGSA
                     MWLVRVALQRPYTFVVMSLLIVILGVVTLFRMPTDIFPNIDIPVISVVFNYGGLVPEE
                     MEKRVVSNYERFLTTTVNNIEHVESQSLTGVAVIKIFFQPGANIDAAVAEVTAVSQTA
                     VRIMPPGATPPLILRYSATNVPILQMALGSESLSEQQIFDLGVNFIRADIATVPGAQI
                     PWPYGGKQRQIMIDLDPRKMYSRGLSPREVNAALGVGNVILPSGTAKMGRNEYPIVIN
                     SSPELLDEIGAIPIKNVNGTTIYIRDVANVRDGNPPQTNMVHVGGRRSVLMSILKLGG
                     ASTLEVVERIRNMLPATLAKVPKELKTSLLFDQSLFVRAAVDGVVREALLAAGLTALM
                     IFLFLGSWRSTLIVVVSIPLSILVSIILLNALGHSLNVMTLGGMSLAVGILVDDATVA
                     IENIHRNMVQRKPFLRAIIDGAQEIAVPAFVSTLCICIVFVPVAFITGAAKYLFVPLA
                     EAVVFAMMTSYFLSRTLVPTMVHYLLKGEAAAHAAEHEASTGPTLDAPRSATKRPSRV
                     ARFFAAFERGFERMRRAYGGLLALALARRAWVVSCFSLFVVASLGLFLLVGRDFFPSV
                     DAGLIKLHVRGQPGTRIEESEAHFLDIENTIRSVIPPAEIETMLDNIGVPYSGINLSL
                     SEGALISSADGQILISLRHGHAKTDDYVQALRTTLRRRYPETTFFFLPPDISTQVLNF
                     GLAAPIDVQLVSPPGQEEKVYAMARRLRDQIAGIPGAADVHLAQVPRVPQLQVNVNRT
                     QASQLGVSERDVASDLLVSLSSSGQVAPSYWLDPKRGVQYLVAVQTPQYSIDSLDALA
                     TTPVSTGANGRPQMLSNFAQFSRSTGAANITHYNVGRTFDVQANVFGTDLASVASGVD
                     RLVNELKPKLPRGVTVSVKGQVESMESSFRGLAFGLIFAVLLVYLLMVVNFQSWLDPL
                     VILMALPGALAGIAWMLFVSRTTFSVPAQMGTIMCVGVATANSILVVTFANGRRKLGS
                     TALEAALAAGMTRLRPVLMTALAMILGMLPMSLGIGEGGEQNAPLGRAVIGGLVFATL
                     STLFFVPVMYSLLRGKPPAPELVIEDA"
     gene            14026..15276
                     /locus_tag="VFQ61_08975"
     CDS             14026..15276
                     /locus_tag="VFQ61_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9954625.1"
                     /translation="MNSQGKIAIPQSASQPAADDLGFDLPGPSNVSRKRMLLVGVAVL
                     GVFGLAFGSAYLPRRAAQHELEARARAASHARPLVAVVAPKLVSSDRKLTLPGTLQPL
                     EEALIYARATGYVRRWHVDIGAKVEKGALLAELDTPEIQQEIAQAQAALAQAQSAVVQ
                     AQASRGLAQIRLERSNKLVAAGVAAQQELDQNQAQATVNDADLKVAESSVVSQRANLR
                     RLSELASFSRVQAPFSGTITQRSVETGSLVSAGGTPLFRLTATDPVRVYVQVPQDLAP
                     SASVGAAASVRLREFPGQDFVGSVARTSGALDPVSRTLSVEIRVPNPEGKLLTGMAAD
                     VSLTLPSPHNVYQIPSTALVTDAKGVRVLLVDGEGRLHSRPVVIERDLGANIQLASGV
                     TETDRVLQIASADFEEGQPVEIKR"
     gene            15313..16848
                     /gene="ilvA"
                     /locus_tag="VFQ61_08980"
     CDS             15313..16848
                     /gene="ilvA"
                     /locus_tag="VFQ61_08980"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HET9954626.1"
                     /translation="MTEDGYLEKILKSRVYDVVSETPLEFAPRLSERLGCHVLIKREY
                     LHSVFSFKIRGAYNKIASLSPEQAARGVVAASAGNHAQGVALAARRLNLKALIVMPAT
                     TPRIKVEAVRAWGAEILLTGDTFDVALAEAERLRAERGLTWIPPYDDPDVIAGQGTVA
                     LEIVRQHRAAIDAIFVPVGGGGLIAGVAAFVKRLYPQTRVIGVEPEDAACLHGALRAN
                     ERILLDRVGIFADGVAVRQVGAEPWKIAQRWVDDVITVTTDEICAAIKDIFEDNRSIA
                     EPAGALAVAGLKAYATTHSLSGQTCVAIESGANIGFDRLRHVAERAELGEAREALLAV
                     TIPERAGSFLGFCESLGRLPITEFNYRYCDPEQAHLFVGVGLSGGVVERQSIIQRVRE
                     GGYPTLDLSDDEMSKTHMRFMVGGRVLGLRDERLCSFEFPERPGALLSFLRELSGRWN
                     ITLFHYRNHGAAFGRVLAGIQVPEAELTSFRETLGRIGYQYREETSNPAYALFLGAQS
                     TNN"
     gene            17047..18081
                     /locus_tag="VFQ61_08985"
     CDS             17047..18081
                     /locus_tag="VFQ61_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9954627.1"
                     /translation="MRYKNLGKTGLFVSELCLGTMTFGGKGFWEAIGKLGASDAETLV
                     GTALDRGVNFIDTADVYSEGESERLLGGALKKLARPREQVVIATKVRGRVGPGVNQVG
                     LSRGHILASIDGSLSRLGLDYVDLYQIHGFDPVTPIEETVEALDAVVRSGKARYVGFS
                     NLPAWVAMKAIGYATANHLARFQSAQMYYSMVGRDIEREIAPMALSEGVAILPWSPLA
                     GGLLSGKYEPDKSGPAGARRSSFDFPPVDHSRLPGVLGALRRVAEAQGVSVARVALAW
                     QLTKPFITSVIIGAKTRDQLLDNLAAADVQLSTEQVQLLDEASALPREYPGWMVEWQN
                     RDVRGVVNPS"
     gene            18219..18815
                     /locus_tag="VFQ61_08990"
     CDS             18219..18815
                     /locus_tag="VFQ61_08990"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9954628.1"
                     /translation="MQRIFFIARHGETDWNLARRWQGHTDIPLNARGREQAAALAERL
                     RGLAVKIERVVTSDLARARETGEIVASCLGIPQLVSDAALRERCFGVFEGLTLEECES
                     RYPEHWSSYRSEAKIPPPGAEPFEAVAARMSEALNRLLVHDAASPNALVIGHGGAMRA
                     FLQSVSGATYPPLENAALFRFVHATTGFADVTHISSSP"
     gene            complement(18829..20013)
                     /locus_tag="VFQ61_08995"
     CDS             complement(18829..20013)
                     /locus_tag="VFQ61_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9954629.1"
                     /translation="MNVHPTLGAFISFDDRSRILEVNEACCRLLGYTREELVGRSFND
                     VLAPSSRLFVQTHVLPMLARIQRFDEAYISVRTRSGEQVPVLLSASRQAPEEGGECHC
                     LFLPMHRRQLFERELLAAQAAAAEARRAEHAALERIQAMQEKLALQERLATVGTLAAG
                     MAHEINNPLGYVATNLQLVEEALRGGVAELDANAMRSMIRDAAEGAERIAKLVRSFKT
                     YSRVELASNAPVDLAHVIDVSVRMTVHEIKARARLELQVTEQPTLVSGDEVRLSQVVV
                     NLLVNAAQAFPRAMPANNQIWLRLDTEADQRVLLEVADNGPGIPRELQERIFEPFFTT
                     KPVGQGTGLGLAICRDIVHSLNGNLSLESEPGRGTRFQIRLPITARGARAPTPLPTGQ
                     AE"
     gene            complement(20010..20771)
                     /locus_tag="VFQ61_09000"
     CDS             complement(20010..20771)
                     /locus_tag="VFQ61_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9954630.1"
                     /translation="MFAHGFGTDQKMWRLVAPAFEPHYRVIRFDYVGSGGADRRAQSD
                     ARYTSLAGYAQDLLEICAALELKEVVLVAHSVSGMIGMLASLEAPELFERMVLVAPSA
                     RYLNDAPNYVGGFDIEEVQGFLDMMDQNFLGWADTFSGIAEKNPETSKQLFDAISTND
                     PRTARRFAEVTFHADVRAELPRVSVPCLLLQCEDDAIVPLVAGEYLRDHLPGCVYHVL
                     PVAGHCPQISAPELIISEIRDFLARPRSISPAPLP"
     gene            complement(21141..22328)
                     /locus_tag="VFQ61_09005"
     CDS             complement(21141..22328)
                     /locus_tag="VFQ61_09005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET9954631.1"
                     /translation="MKFPLDVMIAASVRTPVGRALKGTLRATRPDDLAAVALRAALER
                     VPQVAAEQIEDVVLGCAMPEAEQGLNVARNASFLARLPNSVPGETINRFCSSGLQAIV
                     HAGLAIQAGLTDVALAGGVESMSMVPMSGNKVSLNPKLMDSYPEAYIAMGHTAERVAS
                     RFNVTRQDQDGFAVLSHERALAAIAQGRFQEESVPVKARVFEAGVESEIDFTTDECPR
                     LGTTLEVLGKLKPVFKQGGTVTAGNSSPMNDGAAASLLLSRRRARELGIRPLGVLRGY
                     AVAGVDPDIMGIGPIPAIRKLLTQTGLSIDQIDLFEINEAFAAQAVYCQRELGIPTEK
                     LNVNGGGIALGHPLGCTGAKLTATLLHELKRRGARLGIVSMCIGGGMGAAGLFEAGHS
                     VDL"
     gene            complement(22337..24715)
                     /locus_tag="VFQ61_09010"
     CDS             complement(22337..24715)
                     /locus_tag="VFQ61_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HET9954632.1"
                     /translation="MVAERIRVAGVIGAGVMGSAIAAHLAGAGIRTHLLDIVPPGLSE
                     AERTQALARTRFAQAGLERALSAKPAAFFEPDGARLIQTGNLEDHLDRLSECDLVIEA
                     VVENLELKRKLYGNIASKLSPRAILASNTSGLSIAQLSDALPAALRERFLILHFFNPV
                     RYMRLLEIAPGPSTRSDVLASAVRFGEFLGKGIVYAKDTPNFVANRIGVYSMMLTLHE
                     MQAQGLSVEAVDKITGVPMGRPKSAAFGTADLVGIDTLAHVAKNCLDSLVADEERQVF
                     ALPEFVRELIEAGRLGRKSGAGFYKKAGQDLLVYDPALRDYRAQAKVRFDSLGAIRNE
                     TDPGKRLKMLLGFDDPASRFAWKILSRSLAYSARRIPEIADDIVNIDRAMRWGFNWEL
                     GPFEAWDAIGVPESVSRMQSDGVPVPAWVEQMLSSGQTRFYGGSPAHPTYFDVAQHMP
                     LPVAEDPRELRLAALKSEPSRVVQENAGASLVDLGDGVLGIEFHTKLNTLDGDVIELL
                     TRAPQVAQERGFEAILIGSDGEHFCAGANLMLIAMAAQAKAWDQIENVVKALQGALMG
                     LKYAPLPVVAAPFQYTLGGGCEVALAADARQAHAETYMGLVEVGVGLIPAGGGCLGMV
                     QPYTAGLAALNADPLPFIAQASLNIATAKVSTSAEEAKALRYLSPEDGISLNRARLLH
                     EAKARAWGLARAGYRPPLPRLISAAGYDAARTISARVWGMVEGGFASEHDALIANKVG
                     YILCGGKVASGTLLTEQHYLDLEREAFLSLCGEEKTQLRIQHMLMNGKPLRN"
     gene            25059..26021
                     /gene="pip"
                     /locus_tag="VFQ61_09015"
     CDS             25059..26021
                     /gene="pip"
                     /locus_tag="VFQ61_09015"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HET9954633.1"
                     /translation="MFARRTFYPEIMPYRSGYLQVSPEHSIYFEESGNPRGKPVVFLH
                     GGPGGGTSPKQRRFFDPARYRIVLFDQRGCGKSKPHASLNDNTTWHLVSDIEKLRQHL
                     GVERWMVFGGSWGSTLALAYAETHPERVTELVLRGIFLLRRWELSWFYECGTSRLFPE
                     AFAKYIELIPESERGNLIEAYYRRLTSNDPILRQEAARRWSVWEATTSHLLPDDEYIR
                     SCAGDDFSLAFARIECHYFINGGFLKHENQLLEQVGLIRQIPGVIVQGRYDVVCPAQS
                     AWELAQAWPEARLTMVTDAGHSAYEPGIVHELITATDTFASKSE"
     gene            26028..>29417
                     /locus_tag="VFQ61_09020"
     CDS             26028..>29417
                     /locus_tag="VFQ61_09020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET9954634.1"
                     /translation="MRLQRALSTIKKLCSKRTRRTYLGRNRAHIELPQLSESECEALE
                     SALKRGFAELPKVRFADVHRELSRVVVAFEDDAYTLRELLAIVEGAERAIGVHDAVFR
                     DEPWEHPADGESIERLALGMAVDAASMLVGMGLRATPLPASRLAGTFASVIAIVQSSP
                     RLRRPLDDKLGPNRASLAMNTGSALAYGLAQRPGSALVELLHKASNHSAVRARQRVFE
                     AREPELFDKPRKALPARAPEARPRPLPRGPIEEYADRAWVVSLGGFGVSLLATRSVPR
                     AVAALFGGLPKPARHGRDAFSAHLERDLAERGILVIDREAVRRLDRIDCLVLEGSLVA
                     RDRFQIQAVLSDSNIDDNEVRGLVRELFDATRPIARAEGRGYVLEPLGGSHLEPPAEL
                     RKRAPELGARGGLVLALSRADRILALAEVEWIPQTGIEELIAAARDAQMRVVIASNDE
                     SVLQGFAADDTIADAEGMLRGVRRLQREGRAVCVVATAQSPGLSAADVSIGLLREGEQ
                     PPWGAHVMCRHDLSDVRFLIFACVTARQVAKQSVNVALGAATLGALVSAGGVLPLTAR
                     RVAAVVSGASLVSMANGVRGGMSLARRALPPPRDRTPWQALDAQGVLYRLNTSLEGLT
                     RQKAIERRAKPARTRSALGEFVEAVSDELFNPLAPLLAAGAGLSAAVGSLGDATMVGG
                     VVVLSALVGGVQRYRTERAIRELGKASTRQALVRRAGQLQEIEARELSRGDIVLLGPG
                     DVVPADLRILEAEQLELDASSLTGESLPVRKSAKPSFEAHLADQTSMLYEGMVVVAGR
                     ATAVVVAVGDETEARRNAVAQKRDPSRGGVERRLRSLIDLTGPIALGAGVSLIGSGLL
                     RGRKMEELVGSGVSLAVASVPEGLPLLATAAQLAAASRLAKRGALVRNVRSIEALGRV
                     DVICLDKTGTLTEGRIELAKLSDGQRARKLAELADAERDLLVAALRASEDDQRAGDPI
                     DRALHAAAGSLELGRDVFHKLRSVPYEAGRGYHASLGSAGEDHVLTVKGAPEIVLRFA
                     SDWRRGGELVPIDVATLAQLHQHAADLGREGYRVLAVAERVRRSAVELDPAAPNKLVF
                     LGFLGFRDAIRKTARRAVTELSTTGIRTVMLTGDHPATAKSV"
BASE COUNT         5333 a         9258 c         9437 g         5389 t
ORIGIN      
        1 cttttccaac gtgtgggccc ggcgcaggtg attcggtttc gtgagaagga tacttgcgcc
       61 tcactaggat cgacccgctg acttgaatcg cggaacgtgc ctcacagaca tgcagcatgc
      121 tcggagctcg gcctccgtcg caacattgcc gattcaagat ggacggcgtg attgcggcgt
      181 tgggactgat cggttgcact cgagtcagcg gagctcactg ccgccgcaac ccacaaaact
      241 agttgtaacc aacaccaccc ctgctatact cggacacaca aggaaggggc agacatgtgc
      301 gtttggaaag aaccacggca actgagcctc gacttccagg ccaacccgcg gaattggggc
      361 ggcagacgca aaggggccgg tcggaagggc tccggtagac acattttgca ttcaaaacgc
      421 cacgcccatc gacattatca cccggtgcac gtcacggttc gagtcgccgt gcgttgttta
      481 cgtgcgcagc gcgtcttcgt cgtgattcgt cacgctattc gtgcggtgaa ctcgatacgt
      541 ggcgagcgct tccgcatcgt gcagttctcc gtgcaggcca atcatatcca tctaatcgtc
      601 gaggctgcag aaagggcgga gctgagcaac ggcatccgcg ccctcaatgt ccgcatcggc
      661 aaacacgtca atcggatcct gggtcgcaga gggcgattgg tctcagaaag acatcacgag
      721 cactcccttc aaagcccgcg cgcggtccgc cacgcccttt gctacgttct cgccaacttc
      781 aagaagcatc ccacgcggga gggccaacgt ggagatccgc tcgatccgta ctcgtctgca
      841 caattcttca gtggctttgc cgaaaggttg aacagcggac ctcggtcgtc ccaagactcg
      901 cccgttgcgc ccccacgcac atggctctta cgattcggtt ggcagaggca tggactcatt
      961 tcgatatatg aggcgccacg aagtcactga acgatgtgaa ctcggggcgc acgttgcctc
     1021 tcgccgtcgc aaagccgctg aacctcgcga ccagtatgcc ttcctgcatt tgcctccgcg
     1081 ggaccaccag ctctcgcagg gtgaacgggc cgctcggatc gccccgcgga cgctcgactc
     1141 tcgccggaca agtacgcggc tgacgtccac gcgcgcgatg cttgcgatac gagcggctca
     1201 cgcaatgcat cgcgacacgg accttcggca cgcggtgctg gcgaaacggg ttgctcacgc
     1261 gcacgcgcga tgctcacgat aacggcactg ctcaccgggg ctctctcctc cccgccctca
     1321 cgtgatgctg ccgatacagg tgctcacgcg ggctctctcc ccctcgacag acgtagacaa
     1381 tagtcacgcg ctccccgcgc gtacacacca cggggccgct cacgcgcacc cgcgatagcg
     1441 ataacggcac cgctcacgtg atgctgccga tacaggtgct cacgcgggct ctctcccgct
     1501 cgacagacgt agacaatagt cacgcgctcc ccgcgcgtac acaccgcggg gccgctcacg
     1561 cgcacccgcg atgatcacga taacggcacc gctcacttga gctctctcct ccccgccctc
     1621 acgtgatgct gccgatacag gtgctcacac gggctctctc ccgctcgaca gacgtagaca
     1681 atggtcacgc gctccccgct cgtacacacc acggggccgc tcacccgctc acgtgatgct
     1741 gccgatacag gtgctcacac gggctctctc ccgctcggca gacatagaca atagtgccgc
     1801 gctccccggg cgcgcatacg tggctcgcaa tacgggggcc gctcacgcgc acgcgagatg
     1861 ctttgacgat gcgaacgcgc acgcgcggtg ctgagcacgg ggccgagcac gcttgctcgt
     1921 gaacggctct cgtctgccca atctgagtgc atgcgggcaa ggaggcggca gtgtgttgcc
     1981 gcctccttgc tccaatcacg caggatcgct cggctgactc cgaaaccgtt cgagttgctc
     2041 ctattgcgac gaatacggtc gtgctaccca gacgggcagg cgcggtgttg tgaggggcgc
     2101 gggagatgcg actgagcttg ggcgcgatgt cgacgggcgg tcgcgcacgt cgacagggtg
     2161 gcccaatata accgcggctg agtgggagtc aggatggagg gcgcctgagg gcggagtcgc
     2221 gacggtagcc gaggagggcg cgcttctgga tgacgcgggc aacttcgggc gggacgaggt
     2281 tttcccaatt gggatcgccg gattgaatga gacggaggat gtccgccgcg taaatgcgaa
     2341 ggcattctgg gtcgtagcct tcgagtggga cgatgcagcc gcggtcgatg aggtaaccgt
     2401 agagtttgcg cagttcgggc gcaggttcga agttggcggc ggtgaggagg tcgggctgtt
     2461 tgggatcgag gaagggatat acgtaaagtt tggtgtcgtt cttgaagaga cgaccaaagg
     2521 attcgaggat gccgcctggc agatcgagat aatatctttc gtcgaagagc gggctgaggc
     2581 tgccggcgcc aagtacgatg gcgacgggct tttgagtgta gcgccgcaag tagccgacga
     2641 gccggtagta ctcgaagtag tcggagatga ggacggcttt gcccgcggcg gcgagcatgt
     2701 cggcgcgcgc gaggaagtcc cgatagtcga tgtcaccctg cccactctcg atcaggttgc
     2761 gcatggtgat ttcgctcagt tcgaggatgg gttcgggatc ccctccggcg gcgcgcgtga
     2821 attcgtcgcg agccgtgcgc agcatgtcga ggttggtgcg tgtgaccggg cgaaaggctc
     2881 cgcgctcgac gagcacgggc cgtttgtaga ggacttcgga gggctggagt acgtgccctt
     2941 cggggtcgaa catggcggca ccgctcaggc cgagctcgac gaggcgcagg gccatgactc
     3001 gattgtcgac atggcggaac tcgatgcccg agaactcgat catgtcgatt tcgatgcgat
     3061 cggtgctgag gttgtcgagc agtgagtcga cgagccgatc gggatcgtgg tgcatgaaga
     3121 aagctccgtg gagcaggttc acgccgacta cgccgagcgc ctcctgctgc agcgcgtttt
     3181 cactgtcgag catgcgcacg tggatgatga tttggctgtc ttcgtcgcgc ggatgcgctt
     3241 ggaagcgaac gccgagccag ccgtggcact cgttggtgcc gcggtagctc ttggcggcga
     3301 cggtgtccgc aaaggcgaag aacgcggtgg cgtcaccacg cgcttcacgc aaccgctcga
     3361 ggttgagtcg atgctcgtag ttgagcatgc tctccagacg ttgccggcac acgtagcgct
     3421 gggcagagcc gtagatcgcg tcgctgacgg ccatatcgta agcagagatg ctcttggcga
     3481 tggttcccgc ggcgcctccg acccgaaaga accaacgcac gacttcttgg cccgcgccga
     3541 tctcggcgaa ggtgccgtag cgacgggcat cgaggttgat cgccaacgcc ttttggtgcg
     3601 tgtctagccg ctcggcgctg agtttcttct gacgaggcat tctggcaagc tcactatgaa
     3661 gtccggactt cgtgcacccg ctgtaagcgg catttggttt cgtggcggta tcgcagaacc
     3721 agccttgtaa ggctgaggcg acactgcaaa ctcagaccgg ccaccccacc cgacaaggct
     3781 attccgtgga gattgttggt gagatcgccc accacaagct gcgaagccat cggctaatct
     3841 tgaggtcatg tcgagcgcgg aagcagatgc tctctggtac aaggacgcga tcatctatca
     3901 gctccacgtg cgaagtttct cggacagcac gaacgacggc gtgggtgatt tcaaggggtt
     3961 ggcggatcgt ctcgattaca ttcgagacct cggggtgacg gcgatttggc tgctgccgtt
     4021 ctatccttcg ccgctcaaag acgatggcta cgacatcgcc gattacacca acgtaaatcc
     4081 atcatacggt acgctggccg acttcgagca ctttctgcgc gaggcgcacg atcgcaagtt
     4141 gaaggtgatc accgagctcg tgatcaacca cacctcggac caacacccgt ggttcaagcg
     4201 cgcacggcgt gctcccaagg gtagcgtgga gcgagacttc tacgtgtgga gtgatactcc
     4261 cgatctgtac cgagaagcgc gcatcatctt caaggacttc gagcactcga attggacttg
     4321 ggatccggtg gcgggggcat atttctggca ccgcttctac tcgcaccagc cggatctgaa
     4381 ctttcagaat ccagaggtgc gcaaggcggt gttgaaagcc atggatttct ggttcgcgct
     4441 cggggtggat ggttttcgtc tggacgccgt gccctacttg ttcgagcgcg aggggacgag
     4501 ctgcgagaac ctgctcgaga cgcacctcga gctcaagcat ctgcgctcac acgtggacaa
     4561 tcacttcaag catcgcatgc tgctcgcgga agccaatcaa tggcccgagg atgcagccgc
     4621 gtacttcggc tcgggcgacg agtgccacat ggcctttcac ttcccgctca tgccgcgcat
     4681 gttcatggcg ttgaagatgg aggaccgcta tccggtgctc gacatcttga atcaaactcc
     4741 cgccattccc gacaattgcc aatgggcggt gttcttgcga aaccacgatg agctcacgct
     4801 cgagatggtg acggacgagg agcgcgatta catgtatcgc accttcgcca aggatccgca
     4861 ggcgcgcatc aacctcggca ttcgaaggcg cctcgccccg ctgctcggta acgatcgacg
     4921 ccaaatcgag ttgatgaatg ggctattgct cgccatgccc ggcacccccg tcatttacta
     4981 cggggacgag attggcatgg gagacaatat ctttttgggt gaccgcaatg gcgtacgcac
     5041 accgatgcag tggagcgccg atcgcaacgc gggtttctcg cgcgcgagct cgcagcggct
     5101 ctttttgccg gtgatcaccg aggcgggtta ctcgtacgag gcgctcaacg tggaggcgca
     5161 acacggcaat agcctctccc ttctgtggtg gatgcgaaac ttgatttcgc tgcgtcggcg
     5221 ccaccgcgcc ttcgggcgtg gcacgctcga attcctgtac ccggcgaacc gcaaggtgct
     5281 agcgttctta cgctgccacg agggtgaaga gctgttggtg gtcgccaacc tgtcgcgctt
     5341 cgcgcagtgc gtggagctgg acttgtcttc gcgcgccggg aagatcccgg tggagatgtt
     5401 cgggcacacg cgcttccccg ccgtcgccaa cacgccgtac accctgagct tggcgccgta
     5461 cggcttctat tggttcacgc tggcgcctcc caaactgagc ttggatgagg gggcaccccg
     5521 ggacgagacc aaggatctgc cgaacttcta cgtggaggag acttggctcg atctgctgcg
     5581 gggcccgagc aagcctcaat tcgaagagtt cgtgagtagc ctgctgcctt cgcgccgctg
     5641 gtacgccggt aaggcccgta cgatcaagac cgcggagctg atcgaggtga tcccgctcgc
     5701 cgagaacaac tcgtgtctgg ccctgttccg cgtggattac ttggaaggcg accccgaaac
     5761 gtatctgctc agcatgacct acgcgcgggg cgaacgcgcc cgcgaggtgg cgcaagatcc
     5821 gggctccgcg gtagcgcgcg tctgggtcga cggcgaagaa ggcatcctgt tcagcggcgt
     5881 gcgtgacatg aagctgaaca agacgctgct cgacctgctc agcaccgggc agaagttgga
     5941 agggcggcgt ggcaatctgt ttgcgattac gggcccgagc ggcgccgcgc gcttggccga
     6001 tttgatcgcc aagaactcgc cgctcgagcc caatttgctc tccgcggagc agagcaacac
     6061 ctcgatcgcg ttcggcaaga gcctgatctt gaagctgttc cgcaaggtgg aagaggggcg
     6121 caatcccgac gtggagatcg gggagttcct gacggaagag gcccatttcc cgcacaccgc
     6181 gccgttgctc ggttacctcg agtatcggcc gagcgagggg gaaccctcca cgctcgcggt
     6241 gttgcaagcc ttcgtgccga acgacgggga cgcttggaat tacacgctcg atcacgtgcg
     6301 gcactatttc gagcgcacgc tctcgcgacc cagcgactcg attttgccgg ccgcacccgg
     6361 gccaatcctc acgctcgcca gcggcacaat cccaggcgta gtgcatgagc tcgtaggcgg
     6421 ctatctggag tcggcgcgct tgctcgggca gcgcaccgcc gagatgcatc tggcgctgct
     6481 cactccgacg gaagatccgc aattcaagcc cgagccgttc actccctttt atcagcgctc
     6541 gctcttgcaa tccttccgca atctaaccga agaggtgttc tcgaggcttg gctcgcggct
     6601 agagaccttg gcccccgagg tacagggccg ggctcgccag gtgttgagtc tgaagtcgcg
     6661 gattggcgat gtatttaggg gcatcgtgag ccgcaacgtg tcggcgctcc gcacgcggct
     6721 gcacggcgat taccacttgg gccaggtgct ctggaccgga cgcgacttca tcatcatcga
     6781 tttcgagggt gagccgctgc gcccgatctc ggcgcggcgt atcaagcgta gcccgttgcg
     6841 cgacacggcg gccatgatcc gctctttgaa ctacgccgcg caccaaggcg ttcacaatct
     6901 gcgccgtcaa ggtttgcccg taaacacgaa cttggatctc gatgacttgg ccgagcattg
     6961 gtatcaatgg tccgcctcgg cgttcctgcg ggcgtacttg aggacggccg agggtgccgc
     7021 cttcctgccc aaggatcgcg aggagctggc cttactgctc gatgtgtacc tgctggagaa
     7081 ggccgtttac gagctcgact acgagctccg acaccggccc aactgggtgc acctgccgtt
     7141 gcgcggcatc gagcgcctgg tggcccgttc cgggtagaga caatccgggc cgagatgatc
     7201 gcaagcggcg cgggctcagg gccgcgccgc cgccgcgagg gagccgcctc tcgccttgaa
     7261 acctctcgct tggagaccct gctctcgagc ccgcgtgcgc cgggacatgt aacagcgcgg
     7321 cacgattttc gtgacatact gtcgggaaat gcccagcgtt gtgtgcatcg gcaccggtga
     7381 agcgttcgat cctgcgctcc cgaacaacag cttgttctat cgcggcagca tcaatttgct
     7441 gatcgattgc gggttcgcgg tgcctcaagc cttcttctcg cgggtgcgcg acccttccct
     7501 gctcgatgcg atttacatca cccatcatca cgccgaccac gccttcgggc tgccggccgt
     7561 gttgttgtgg atgaaggaag agggccgaac gcgaccacta gaactgattg ggggcccagg
     7621 gttggctgag tatgccgaga agttgatgac cctggcttat ccagggtcgt tcgaagattg
     7681 ttaccccatc attcctgtgg aattgacccc aggccgcgtg ctgaagagag gttcggccga
     7741 gttacgcaat gctcggtcgt cccatcgcgt gcggaacttg gcgttgcgca tcgacgattt
     7801 ggggcgatcc ttttgccata gcggggacgg agcaccgacg ccggagactc tcagcctcta
     7861 tcggcgcgcc aacctgcttt cacacgagtg ttacttcgcg gagcaggaga ccgagagcca
     7921 cgcgcacttc gccgaggttc tggcgctcgc cgaagtggcg cacgtggaga ctctgatgct
     7981 cacgcatttg gggcggaaag agaaggagcg tattcaccag ctcgccatga gtacgccgcg
     8041 caaggtgatc attccaaagc caggcgctca tatccatatc tgagtcagcc cgtatcggag
     8101 tgcgaagtcc aggctcagcg cgtgcgccgc tggatgtagc gccgcaggaa ggcgcgcacc
     8161 tgacgagggc cgtccaagcg gtagcgcgct cgcgtgggca gcggccccgc gcagaaggtg
     8221 aaacgcgcgt ccggaataga ggcgaacaag tcctcgtccg tgcgatcgtc tccgaacgcc
     8281 gccagggcac gcgcgccggg cagttcggcg agtacactgc gcagcgactg acccttgtcg
     8341 atcccttgcg ggcgcacctc tacgaccttg tgcccggtga gaatgttgac cgggctctgg
     8401 gcgagggttt ggaccaagtg catgcggagc tcgcgcgagc gaaccgcccc caagtggggg
     8461 gccgcgttgc gatagtgcca agccaaaccc gccgactttt cctcgatcat ggctccgggc
     8521 gtgcgcacca tgtactcctc gaagatcgcg cgcaccatgc tgcgaaagcc agagcccgag
     8581 aagtcgaatt gcgcgtggaa ctccgcctca ccgagacggc gcacgaacac tccatgttca
     8641 gcgatcatgc cgatcggcag ctggccgagc caagcttcca tgctctcgcg cgtgcgcccg
     8701 ctcacgacgt agacggaggt tcgcggcgca cgcgcgagtt cgccgagcag ctccaagagc
     8761 tcgccgtcgg ggaacgcgag ttcggggcgc gcgcgtagcg ggaccagcgt gccgtcgtaa
     8821 tcgatggcga gcacgacctc cggctcgagc gcgagctcac ccgcaagttc atctacctcg
     8881 acgtcatcca acggcgcatc tcgctgcagt gcgtccgcgg ggcgcacctc gagcgcgctc
     8941 aagtagctct ccgcccaaac ctgcaccgga cgcgccaaga ctttctgacg tagaaccgcc
     9001 atgcgatgcc gacgctcgga ttccggcatg ctgagcgccc tttcgaagct cgcggcgagc
     9061 gcctcgacgt cgtacggatt taccgtcaac gcttcgccga gctcgctcgc cgccccggcc
     9121 agctccgaga gcaccaatac gccgtcgccg tcgacgcgcg aggcaatgaa ctccttggcc
     9181 accaagttca tcccatcacg taaaggagta acgaccatga cgtccgccgc gcggtagctc
     9241 gccgccagct gattacccgt gaccgactga tacagatagt gaacgggggc gccggctagg
     9301 gttccatgag cgccgttgac tcgacccacg agctcctcca cgtctcgacg caagcttttg
     9361 taagcgtcga ccttctcgcg cgaaggcacc gccacttgca gcaagcgcac gcgaccgcgc
     9421 agcgccggtg ttctctcgag tagacgctcc atcgccaaga ccttgcgggg gatgcccttg
     9481 gtgtagtcga gccgatctac gccgagcagc aggcgttcga cgccgtgggc ggcccgcagc
     9541 gtctgcatct cggccagcac ttcgtccgcg gcggcacgct ccgcgaaggt ggtcgcgtcg
     9601 atgcccatcg gaaagacgcc gagccgtact tcgcgaccat ccacctgcag gtggtcgagc
     9661 tcgacctcca gccccaacag gccaagcagc gaatccgaaa agtgccgcaa atagtcatac
     9721 gagtggaagc caatcagatc ggcacccaag agcccgtgca gcaccatgtc gcgctgcgga
     9781 agggtgcgga agacttcgga cgcggggaaa ggaatgtgta gaaagaagcc gatcgtcgcg
     9841 tggggaagcc gcgcgcggag catggctggc agcagcatga gttgatagtc gtgaatccag
     9901 atcacgtccc cgtctcgggc gtgactcacg accacgtcac agaagcgccg attggcttga
     9961 acgtaggcat cccagtgccg aatgacgagc ggaacgcggt cgatcagata gtggaagagc
    10021 ggccacaaaa cgccgttcgc cacgccttcg tagaaacgtt ggctctcgtg gctcgagaga
    10081 tgtactggga ccgcgtccag ctccgccagg cgctgatcca tgaggaaacg ctgctcgtcg
    10141 ctccgggccg ccgcaccggg ccaaccaatc cagagtgtgc ggcccccact cgcacgaaac
    10201 ccctgcaacc cggtggccag gccgccgctg cttggctcca cctcgaggcg atcaccatcc
    10261 aatttgacgg caaccggcaa gcggttggag accaggatga cccggcggtc gattttttgc
    10321 acttccacaa ctcgtttata gcctgtttaa gactttatca gggtatgtcg gcgctgcggg
    10381 ttttgcccca agtcagtcga acgcgcggcg attcacgtcg tgggcgctcg cgcaggtttg
    10441 gagcatgaat cgcggagcac agctcgcgct cgagcacctg aacgaatgat tctgcgggaa
    10501 gtcaagcgag ggccgccgct gcaatcgctg aaatgcgccc gcgcgtcgga ccgagagcgc
    10561 tcacagctaa gacatttcgc cacaatcgca gaagacagta gatctgaaac cagttccgaa
    10621 gcggcccggc ctcgatgacc cccaagcggg actgaagctt cgtttcagcc gaaccggtga
    10681 gcaaacgctg gctcacgcaa actcgtctag aacacggcgc cggggcgttg acgttggaag
    10741 gcgctggccc taaggggacc gctgcattcg aggtctttcg acgctccttc gatggttctg
    10801 gtagcgcaat gtggctagtt cgagtggcgc tccagcgccc ttataccttc gtagtgatgt
    10861 ccctgctgat cgtgatcctg ggggtggtca cgctcttcag aatgcccacc gacatcttcc
    10921 cgaatatcga catcccggtc atctcggtgg tgttcaacta cggaggattg gtgccggaag
    10981 agatggagaa gcgcgtcgtg agcaactacg agcgcttcct caccacgacc gtcaacaaca
    11041 tcgagcacgt cgagagccag tcgcttacgg gggtggccgt aatcaagatc ttcttccagc
    11101 ccggcgccaa catcgacgcc gcggtggccg aagtcacggc cgtgtcgcag acagccgtgc
    11161 gcatcatgcc cccgggagcg acgccgccgc taatcctccg gtatagcgcg accaacgttc
    11221 cgatcttgca gatggcgctc ggcagcgaat cgctcagcga gcagcagatc ttcgacttgg
    11281 gcgtgaactt cattcgcgcc gatattgcga ccgtgcccgg cgcgcagatt ccctggccct
    11341 acggcggcaa acagcgtcag atcatgatcg atctggaccc gcgcaagatg tactcacgcg
    11401 ggctgtctcc gcgggaggtg aacgccgcgc tcggggtggg gaacgtgatt ttgccctcgg
    11461 gcaccgccaa gatggggcgc aacgagtacc ccatcgtcat caacagcagc cccgagctgc
    11521 tggacgaaat tggggcgatc ccaatcaaga acgtcaacgg tacgacgatc tacatccgcg
    11581 acgttgccaa cgtccgcgac ggcaatcctc cccagaccaa catggtgcac gtgggcgggc
    11641 ggcgctcggt gttgatgagc attctgaagc tcggtggagc cagcaccttg gaggtggtcg
    11701 agcgcatcag gaacatgctg ccggccacgc tcgcgaaggt gcccaaagag ctcaaaacct
    11761 cgctgctgtt cgatcaatcg ttgttcgtgc gcgcggcggt ggacggcgtg gtgcgcgagg
    11821 ccttgctggc cgcgggcctg accgcgctga tgatctttct gtttctcggc agctggcgca
    11881 gcaccttgat cgtggtggtg tcgatcccac tctcgatctt ggtctcgatc attttgctga
    11941 acgcactcgg gcactcactc aacgtgatga cgctcggggg gatgtctctg gccgtaggga
    12001 tcttggtgga cgacgccacg gtggcgatcg agaacattca ccgcaacatg gtccagcgga
    12061 agccattctt gcgcgccatc atcgacggtg cgcaagagat cgcggttccc gctttcgtat
    12121 ccacgctgtg catttgcatc gtgttcgtgc cggtggcctt catcacgggg gccgccaagt
    12181 acctgttcgt gccgctcgcg gaagccgtcg tgttcgcgat gatgacttcc tacttcctgt
    12241 cgcgaacgct cgtaccgacg atggtgcact atctgctgaa aggcgaagcg gcggcccacg
    12301 ccgccgagca cgaggcgagc accggcccca cccttgatgc gccacgttca gcgacgaaac
    12361 gcccgtcccg ggtcgctcgt ttcttcgccg cgttcgagcg cggcttcgaa cgcatgcgcc
    12421 gcgcctacgg agggctgctg gcgttggcgc tcgcccgccg cgcgtgggtg gtgagctgct
    12481 tttcgttgtt cgtggtcgcg tcactaggct tgttcctgtt ggtggggcgc gacttcttcc
    12541 ccagcgtgga tgcgggcttg atcaagctgc acgtgcgcgg tcaacccggc actcgcatcg
    12601 aggagagcga agcccacttc ctcgatatcg agaacacgat ccggagcgtg atcccacccg
    12661 ccgagatcga gacgatgttg gacaacatcg gagtgcccta tagcggcatc aacctgtcgt
    12721 tgagtgaggg cgcgctgatc tcgtctgccg acgggcagat tctgatctcg ctacgccatg
    12781 ggcacgccaa gaccgacgac tacgtgcaag cgctacgcac gacgttgcgt cgtcggtatc
    12841 ccgagacgac gtttttcttc ctgccgccag acatctcgac gcaggtgttg aacttcgggc
    12901 tcgccgcgcc gatcgacgtg cagctggtga gcccgcccgg ccaagaagag aaggtatatg
    12961 cgatggcccg tcgcttgcgc gaccaaatcg cgggcatccc gggcgcggcc gacgtccacc
    13021 tggcgcaggt tcctcgcgtg ccgcaattac aagtgaacgt caaccgcact caagcttcgc
    13081 agctgggcgt gagcgagcgt gacgtagcga gcgacttgct ggtttcgttg tcttcgagtg
    13141 gtcaggtggc gccgagctat tggttggatc cgaagcgggg cgtgcaatac ttggtggcgg
    13201 ttcagacccc ccagtactcg atcgactcac tggacgcgtt ggcgaccacg cccgtctcga
    13261 caggcgccaa tggccggccg cagatgctct ccaatttcgc tcaattttcg cgttccaccg
    13321 gggccgcgaa cattacccac tacaacgtgg ggcgcacctt cgatgtgcag gccaatgtgt
    13381 tcgggaccga tttggcgtcg gtcgcttcgg gcgtggatcg gctggtcaac gagctcaagc
    13441 cgaagctgcc gcgcggggtg acggtgagcg tcaaagggca agtcgagagc atggagtcgt
    13501 ctttccgcgg cttggctttc ggcctgattt tcgcggtact cctggtttac ctgctgatgg
    13561 tcgtaaactt ccaatcttgg ctcgacccgc tcgtgatttt gatggctctg cccggtgcgc
    13621 tcgcgggcat cgcctggatg ctgttcgtgt cgcgcacgac gttcagcgtg ccggcgcaga
    13681 tgggcaccat catgtgcgtg ggcgtcgcga cggccaatag catcctagtg gtgacgttcg
    13741 cgaatgggcg acgcaaactg ggcagcaccg cgcttgaagc cgcactggcc gcaggtatga
    13801 cgagactccg cccggtgttg atgaccgcgc tggcgatgat cctcgggatg ctgccaatgt
    13861 cgctcgggat tggagagggc ggcgagcaga atgccccgtt gggacgcgcg gtgatcggtg
    13921 gcctggtgtt tgccacgctt tcgacgttgt ttttcgttcc cgtgatgtac agcctgctgc
    13981 gaggcaagcc gccggcgccg gaactggtga tcgaggacgc ataacatgaa tagccaaggc
    14041 aaaatagcca taccgcagtc cgcctctcag cccgcggctg acgatcttgg tttcgatctg
    14101 ccggggccga gcaacgtttc gaggaagcgc atgctcttgg tcggggtggc ggtgctcggt
    14161 gtgttcgggt tggccttcgg tagcgcatac ttgccgaggc gggccgccca acacgagctc
    14221 gaagcgcggg cacgagcggc ctcgcacgcg cggcctttgg ttgcggtcgt cgcgcccaag
    14281 ctcgtctcga gcgatcgcaa gctcaccctc ccgggtacgc tgcagccgct cgaagaggcc
    14341 ctgatctacg cgcgcgccac cggctacgtg cgccgctggc acgtagatat cggagcgaaa
    14401 gtggagaagg gcgcgctgct ggccgagctc gacacccctg agattcagca ggagatcgcg
    14461 caagcgcaag ccgcgctcgc gcaagcgcag tccgccgtgg tgcaggcgca ggccagccgc
    14521 gggctcgcgc agataaggct cgagcgctcg aacaagctcg tagccgcggg ggtggccgca
    14581 caacaggagc tcgatcagaa tcaagcccaa gccaccgtga atgacgccga cttgaaggtg
    14641 gcggagtcga gcgtggtatc ccaacgcgcc aacctgcgcc ggctatccga gctcgccagc
    14701 ttttcgcgag tgcaggcgcc gttcagcgga acgatcacgc aacggtcggt cgagacgggg
    14761 agcctggtga gcgcgggagg cacgccgctg ttccggctga ccgcgacgga tcccgtgcgt
    14821 gtttacgtgc aagtcccaca agatctcgcg ccttccgcga gcgtgggcgc cgcggccagc
    14881 gtgcgtctgc gggagtttcc cgggcaggat ttcgtgggca gcgtcgcgcg gacgtccggc
    14941 gcactggatc ccgtttcacg cacgttgagc gtcgagatcc gcgtgcccaa tccagaaggc
    15001 aagctgctga ccgggatggc cgcggacgtg agcctaaccc taccgtcgcc tcacaatgtc
    15061 taccaaatcc catccaccgc cctggtcacg gacgccaagg gcgtgcgcgt gctgctggtg
    15121 gacggagagg gccggctgca ctcacgaccg gtggtgatcg agcgagatct cggcgcaaac
    15181 atccagctcg cgagtggtgt tacggagacg gatcgagtac tccaaatcgc cagcgccgac
    15241 ttcgaggaag gccaacccgt cgagatcaag cgctgactga gctccgaccc gtagacaccc
    15301 cgtagacgtt gaatgaccga agacggctat ctcgagaaga tcctcaaatc gcgagtttac
    15361 gacgtcgtca gcgaaacgcc gctcgagttc gcgccgcgct tgtccgagcg gctcggctgc
    15421 cacgtcctga ttaaacgaga atacctgcac agcgtcttct cgttcaagat tcgaggcgcg
    15481 tacaacaaga tcgcgagcct ctcgccggag caggcggcgc gtggggtggt cgccgcgtct
    15541 gccggcaatc acgcgcaggg cgtcgcgctg gcagctcgtc gcctgaacct gaaggcgctg
    15601 atcgtgatgc ccgccaccac gccccgcatc aaggtggagg cggtgcgcgc ctggggcgcc
    15661 gaaatcttgc tcacggggga cacgttcgat gtcgcgctcg ccgaagccga gcggctgcgc
    15721 gccgagcgcg gcttgacctg gatccctccg tacgacgacc ccgacgtgat tgctggacaa
    15781 ggcacggtcg ccctcgagat cgtgcgccaa caccgggccg ctatcgacgc gatcttcgtc
    15841 ccggtgggcg gcggggggct gatcgcggga gtagcggcgt tcgtgaagcg gctctacccg
    15901 cagacgcgcg tcatcggcgt agaacccgaa gacgcggctt gtttgcatgg ggcgttgcga
    15961 gccaacgagc ggattctttt ggatcgggta ggcatcttcg cggacggagt tgcagtacgt
    16021 caggtgggcg ccgaaccgtg gaagatcgcg caacgctggg tggacgatgt gatcaccgtg
    16081 acgaccgacg agatttgcgc agccatcaaa gacatcttcg aggacaatcg ctcgattgcg
    16141 gagccggcgg gtgcactcgc cgtcgcgggt ctcaaggcct acgcgactac acactcgctt
    16201 tccggccaaa cctgcgtagc gatcgagtcg ggcgccaaca tcggattcga tcgactccgg
    16261 cacgtggccg agcgcgccga gttgggcgaa gcgcgtgagg cgcttctggc cgtgacgatc
    16321 ccggaacgcg cgggcagctt tctgggattt tgcgagtcac tgggacgctt gccgatcacc
    16381 gagttcaatt accggtattg cgatcccgag caggcccatt tgttcgtcgg ggtcggcttg
    16441 agcgggggcg tggtggaacg ccagtccatc attcagcgtg tccgcgaagg tggctatccg
    16501 acgctcgatt tgagcgatga cgagatgtcc aaaacgcaca tgcgtttcat ggtgggcgga
    16561 cgcgtgctcg gccttcgtga cgagcgcctc tgtagcttcg agtttccaga acgccccggt
    16621 gctctcctga gcttcctgcg cgaactgagc ggacgttgga atatcaccct gtttcactac
    16681 cgcaaccatg gcgccgcgtt tggccgtgtg ttggccggaa ttcaggtgcc cgaagccgag
    16741 ctgacttcgt ttcgcgagac gctcggccgc atcggctatc aatatcgcga ggagacctcg
    16801 aatcccgctt acgctctgtt cctaggagcc cagtcgacga acaactgagc gctcactccg
    16861 ggcacatcag ggtagcccgc cggctttcat gaccggtccg gggggaacaa tcgggctgcg
    16921 tgcttgcggt gcgtggccag gagctctaaa tcggagatcc ccgaggcggc caggccgcct
    16981 cgagaatcgc tcgttgcgct cacgcaagtg cgcgcggggg ccccgctccc gtagttcgga
    17041 ggaatcatgc gttacaagaa cctcggcaag accggcctgt tcgtttccga gctgtgcctc
    17101 ggcacgatga ctttcggagg caaaggcttt tgggaggcca tcggcaagct cggcgcgtcg
    17161 gacgccgaaa cgctcgtcgg cacggcgctc gatcgtggag tgaacttcat cgacaccgcc
    17221 gatgtgtatt cggaaggtga gtccgagcgg ctgttgggcg gggctttgaa gaagctcgca
    17281 cggccgcgcg aacaggtggt gattgccacg aaggtgcgag gccgcgtggg cccgggcgtg
    17341 aaccaagttg gcctatcacg cgggcacatt ctggccagca tcgacgggag tttgagtcga
    17401 ctgggcctcg attacgtcga cctttaccaa atccacggct tcgatccggt aactccgatc
    17461 gaagaaacgg tggaagcgct ggacgcggtg gtgcgctccg gcaaagctcg ctacgtcggc
    17521 ttcagcaatc tccccgcgtg ggtggccatg aaagcgatcg gttacgccac tgcgaaccac
    17581 ctcgcccgtt tccagagcgc gcaaatgtat tactcgatgg tgggtcgcga catcgagcgc
    17641 gagatcgcgc caatggcgct gtcggaaggc gtcgcgatcc tgccgtggag ccccctcgcg
    17701 ggcgggctct tgtccggcaa gtacgaaccg gacaagagcg gtccggcggg cgcacggcgt
    17761 tcctcgttcg acttccctcc ggtggatcac agccggttgc caggggtgct cggcgcgcta
    17821 cgtcgcgtcg cggaagctca aggcgtgtcc gtagcccgcg tggctctggc gtggcagctc
    17881 accaagccct tcatcacgag cgtcatcatc ggcgccaaga cccgtgatca gctcctcgat
    17941 aacctggccg cggcggacgt acagctctcg accgaacaag ttcagctgct cgacgaggcg
    18001 agcgctctgc cgcgtgaata tccgggctgg atggtcgagt ggcagaaccg agacgtgcgc
    18061 ggcgtcgtca acccgtcgta gcggtgccac gcgagtcgca gggtgcctgc tctccctctc
    18121 gggggtgggt tgggcccctg cgtccctctc cttggtgcgg ggtgagacga acttcggggc
    18181 gaacgggccc tctagcgcgg cgatgatagg ctttcgcggt gcagcgcatc ttcttcatcg
    18241 cccggcatgg tgaaacggac tggaatctag ctcgccgttg gcaaggccat accgacatcc
    18301 ctctcaatgc tcgagggcgg gagcaagcgg ccgcgctcgc ggagcgactg cgcgggttag
    18361 ccgtcaagat cgagcgagtg gtgaccagtg acttggcacg ggcgcgggag acgggtgaaa
    18421 tcgtcgccag ttgcctgggc attccgcagc tcgtcagtga cgctgcgctg cgcgaacggt
    18481 gctttggtgt gttcgaaggc ctcaccctcg aggaatgtga gtctcgttac ccagagcact
    18541 ggtcgagcta tcgttccgaa gcgaagatcc cacctcccgg tgctgaaccg ttcgaagcgg
    18601 tcgcggctcg catgtcggag gcgctcaatc gattgctcgt gcacgatgcc gcttcgccca
    18661 acgcgctcgt gattgggcac ggcggagcca tgcgggcctt cttgcaatcg gtgagcgggg
    18721 ccacctaccc tccgctcgag aatgcggcgc tattccggtt cgtgcacgcg actaccggct
    18781 tcgccgacgt cacgcacatc agcagctcgc catagcggcg cacttcgcct attcggcctg
    18841 gccagtgggc aggggagtcg gcgcgcgggc gccacgtgca gtgatgggca ggcgaatttg
    18901 aaagcgcgtg ccgcgcccgg gctcgctttc cagagacaag ttcccgttca gcgagtgcac
    18961 gatgtcacga cagatcgcca gaccgagacc ggtaccttga cccaccggct tcgtggtgaa
    19021 aaatggctcg aagatgcgct cctggagctc gcgcggaatg cctgggccgt tgtccgctac
    19081 ctccagcagt acacgctgat cggcttcggt atcgagccgg agccagattt gattgtttgc
    19141 cggcatggcc ctcgggaagg cttgagctgc gttcacgagc agattgacca ccacctggct
    19201 cagccgcact tcgtcaccgg agaccagagt gggctgttcg gtgacttgga gttcgagccg
    19261 ggcccgggcc ttgatttcat gcacggtcat gcgcacggag acgtcgatga cgtgggccag
    19321 atcgaccggc gcgttgctcg cgagttccac gcgcgagtaa gtcttgaagc tgcgcacgag
    19381 cttcgcgatg cgctctgccc cctccgccgc gtcccgaatc atcgagcgca tcgcgttggc
    19441 gtccagttcc gcgaccccac cccgcagcgc ctcctcgacc agctggaggt tggtcgccac
    19501 gtagccgagc gggttgttga tctcgtgagc catgcctgcc gccaaagtgc caacggtcgc
    19561 gagccgctcc tgtaaagcca atttttcctg catggcttgg atccgctcga gcgcggcgtg
    19621 ctccgcgcgg cgcgcctccg cggcggcggc ctgggctgcc aagagttccc gctcgaacag
    19681 ctgccgccga tgcatcggga ggaacagaca atgacattcg cctccctcct cgggagcctg
    19741 ccgagacgcg ctcagcagta ccgggacttg ttctccggag cgggttcgga ccgagatata
    19801 cgcctcatcg aagcgctgaa tgcgcgccaa cataggcaag acgtgcgtct gcacgaagag
    19861 ccgagagctg ggcgccagaa cgtcgttgaa agagcggccg accagctcct cgcgggtgta
    19921 gcccaatagc cggcagcacg cctcgttcac ctcgagaatg cggctccggt catcgaatga
    19981 gatgaacgcg cccagcgtcg gatgcacgtt catggcagag gggctggcga tatcgaacgc
    20041 gggcgggcca gaaagtctcg gatttccgag atgattagct ccggcgcact gatttgcggg
    20101 caatgccccg caaccggcag cacgtgatac acgcatccgg gcaggtgatc ccgcaggtac
    20161 tcccctgcga cgagcggcac gatcgcgtca tcctcgcact gcaacaacaa acatgggacg
    20221 ctgacccgtg gaagttccgc ccgaacatcc gcatgaaatg tcacttccgc gaagcgacga
    20281 gcggtgcgtg ggtcgttggt cgaaatcgcg tcgaatagct gcttcgaggt ttcggggttc
    20341 ttctctgcga tccccgaaaa cgtatcggcc caacctagga aattctggtc catcatatcg
    20401 agaaagcctt gcacttcctc gatgtcgaaa ccgcctacgt agttcggcgc atcgttcaag
    20461 tagcgcgccg atggcgccac gagcaccatc cgctcgaaca actcgggtgc ttccagcgaa
    20521 gccaacattc cgatcattcc gctgaccgag tgcgcgacca gcacgacctc cttcagttcg
    20581 agggccgcgc agatttcgag cagatcctgc gcgtatccgg caagcgacgt ataccgcgcg
    20641 tcggactgcg cccttcgatc ggccccgcct gagcccacgt aatcgaagcg aatgacacga
    20701 tagtgcggtt cgaaggcggg ggcgacgaga cgccacatct tctgatcggt tccgaagccg
    20761 tgggcgaaca agatcgtccg atctccttca ccggagattc gaacgttgtt gcggacacgg
    20821 atattcgaga cgctcgattg gttatttgct ctgtttgccg agcacgccct ggtttccccg
    20881 tgagccccgc tttcccggga agcggagtgc tgtattgaac ctgtagtcat cgtcccaccc
    20941 tgagttgaat ccctcgaaac tctccatgct atcgcggcct tttactggcg ttctttccag
    21001 cgcgtggccg cgcgagaaac gcgctgcggc tgacaactag cacgcagtaa acgcatgccg
    21061 tagcaaagaa cctggtagaa ccggcattga tcagcgtatt ggaacgacgt ggcgaccgcc
    21121 aggcggattc cgtcagccgc tcaaagatcg acagagtgcc cggcttcgaa caagcccgca
    21181 gcgcccatgc cgccaccgat acacatggat acgattccca agcgagcgcc tcgccgcttc
    21241 agttcatgaa gcaacgtcgc ggtcagtttc gcgccggtgc acccgagcgg gtgccccaaa
    21301 gcgatgccgc cgccgttgac gttgagcttc tcggtgggaa taccgagctc tcgttgacaa
    21361 tacacggctt gagccgcgaa cgcctcgttg atctcgaaca gatcgatttg atcgatactc
    21421 aagcccgtct gagtcagcaa ctttcgtatc gcggggatcg ggccaatacc catgatgtcc
    21481 gggtcaaccc cggcaacggc gtatccacgc agcacgccga gcgggcgaat gcccagctcg
    21541 cgcgcccggc gacggctcag cagcaggctg gccgccgccc cgtcgttcat cggtgaggaa
    21601 ttgccggccg taaccgtgcc accctgtttg aacacgggtt tgagcttgcc gagcacttcg
    21661 agcgtggtgc cgaggcgggg gcattcgtcg gtcgtgaagt cgatctcgct ctcgacgcca
    21721 gcttcgaata cgcgagcttt gaccggcaca ctttcttcct ggaatctgcc ctgcgcgatc
    21781 gcggccaagg cccgctcgtg cgacaacacg gcaaagccat cttggtcttg ccgtgtcacg
    21841 ttgaagcggg acgcgacgcg ttccgcggtg tgccccatgg caatataggc ttctggataa
    21901 ctatccatca gcttgggatt caagctcact ttgttgccgc tcatcggcac catggacatg
    21961 gactcgactc cacccgccag ggcaacgtcg gtcaaaccgg cttgaatggc gagccccgca
    22021 tgcacgatgg cttgcagccc gctcgagcag aaacggttga tcgtctcacc cggaacggag
    22081 ttcggcaaac gtgccaagaa tgaggcgttc cgcgccacat tcaagccctg ctccgcctct
    22141 ggcatcgcgc agcccaacac gacgtcttca atctgttcgg ccgccacctg cggcacgcgc
    22201 tcgagggccg cgcgcaaggc aacggcagcc aagtcatcgg gacgggtcgc gcgcagcgtg
    22261 cccttgaggg cgcgcccgac cggagttcga acggacgcgg cgatcatcac atctagaggg
    22321 aatttcatgg ctgtcctcag ttgcggagcg gttttccgtt catcagcatg tgttggatac
    22381 ggagctgcgt cttctcttcc ccgcacaggg acaagaaggc ttctcgttcg agatcgaggt
    22441 agtgttgctc cgtgagcagc gtgccactcg caactttgcc accgcacaag atgtagccca
    22501 ccttattggc aatcaaggcg tcgtgctcgc tcgcaaagcc gccctcgacc atgccccaca
    22561 cccgcgcgga gatggtgcgc gccgcatcgt aacccgcggc gctgatcagg cgaggcaatg
    22621 gcgggcgata gccggcccgg gcgaggcccc aagcccgagc tttggcctcg tggaggagcc
    22681 gcgcgcgatt gagcgagatc ccgtcttccg gggacaagta gcgtaaggct ttggcctcct
    22741 ctgcactcgt agagaccttg gccgtcgcga tattcagaga agcctgggca atgaagggca
    22801 atggatcagc gttgagcgcg gccaagccgg ccgtgtaggg ctgcaccatg ccgagacagc
    22861 cgccccccgc ggggatgagg ccaaccccca cctcgacgag ccccatgtag gtttccgcgt
    22921 gggcctggcg agcgtcggca gcgagggcaa cctcacaacc accgcccagc gtgtactgga
    22981 acggcgcggc aaccaccggc aagggagcgt acttcaagcc catcaacgct ccttgtaagg
    23041 ccttcacgac gttctcgatc tggtcccaag ccttggcctg ggcggccatg gcgatcaaca
    23101 tgaggtttgc gccggcgcag aagtgctcac cgtcgctgcc gatcaagatc gcctcgaaac
    23161 cgcgctcttg agcgacctgc ggcgcacggg tcaggagttc gatgacgtcc ccgtccagcg
    23221 tgttgagctt ggtgtggaac tcgatcccga gcacaccgtc acccagatcg accaagctag
    23281 cgcccgcgtt ctcctgcacg acgcggctgg gctctgattt cagagcggcc aggcgaagct
    23341 cgcgcgggtc ctcggctacg ggtagaggca tgtgctgagc gacgtcgaaa taggttggat
    23401 gggccgggct acccccataa aaccgagtct ggcccgagct cagcatctgc tcgacccacg
    23461 cgggcaccgg cacgccatcg ctctgcatac ggctcacgct ctcgggcact ccgatcgcgt
    23521 cccatgcctc gaacgggccc aattcccagt tgaaccccca gcgcattgcg cgatcgatgt
    23581 ttacgatatc gtccgctatc tcggggatcc gccgcgcgct gtacgcgagc gaacgcgaca
    23641 agatcttcca agcgaaacgg ctcgccggat cgtcgaatcc gagtagcatc ttgagccgct
    23701 tgccaggatc ggtctcgttg cgaatcgcgc ccaacgaatc gaacctcacc ttggcttggg
    23761 ctcggtagtc ccgcaacgcg ggatcatata cgagcaagtc ttggccggcc ttcttgtaaa
    23821 agccggcgcc gctcttgcgc ccgaggcggc ccgcttcgat cagctcgcga acgaactccg
    23881 gtagcgcgaa cacctggcgt tcttcgtccg ccaccagcga gtcgaggcag ttcttggcca
    23941 cgtgagccaa ggtatctata ccgacgaggt cggccgtacc gaacgcggcg ctcttcgggc
    24001 gtcccatcgg caccccggtg atcttatcga ccgcctccac gctcaagccc tgcgcttgca
    24061 tctcgtgcag agtgagcatc atcgaataga cgccgatgcg attggccacg aagttcggcg
    24121 tgtccttggc gtaaacgatg cccttgccga gaaactcccc gaagcgaacg gcgctcgcca
    24181 aaacgtccga tcgcgtgctc ggtcccggcg cgatctccaa tagccgcatg taacgcacgg
    24241 gattgaagaa gtggagaatc aaaaagcgct cacgcagcgc ggcgggcagc gcatccgaca
    24301 actgcgcgat ggaaaggccg ctggtgttgg aggccaagat cgctcggggc gaaagcttgg
    24361 acgcgatgtt gccgtagagc ttgcgcttga gctccaggtt ctccaccacc gcctcgatca
    24421 cgagatcgca ttcgctcagg cgatccaggt gatcttcgag attgcctgtc tgaatcaagc
    24481 gcgctccgtc cggctcgaag aacgcggccg gtttggccga cagcgcgcgt tccaggcccg
    24541 cctgcgcaaa ccgcgttcgg gccagagctt gcgtgcgctc cgcctcggag aggcccggcg
    24601 gaacgatgtc caacaggtgg gtgcgaatgc ctgcgcccgc caaatgcgcg gcgatggcgc
    24661 tgcccatcac tccggcaccg atgactccag cgactcgaat gcgttcggcg accatcttgt
    24721 ttcggatcgt ggagcgattc ggtttcgaat gccagaggca aacagaccca gtccgagtag
    24781 ttggacggcg gagcggatcg agcagccgaa tgacctgggt ggacgcagag ctatcctctc
    24841 ggtcctctac tcggaccccg ctccaagctc cggtcggtcc taagctctga gcccgcgcct
    24901 aacctccggc cgagcctaca cgtaacccca agccaagatg caggcaccat aaccccgagc
    24961 tcgtgcgagc tctgttcggc aaatgagatc gagccgcgtg aaatcgagca gcgctgacgc
    25021 tacttcggcc gcgaaaagcg cgctaggctc cggccctcat gttcgcgcgg cgcacctttt
    25081 atccggagat catgccgtat cgcagcggct atctccaagt ctctccggaa cactccatct
    25141 atttcgaaga gtcgggcaat cctcgcggca agccggtggt gttcctccat ggcgggcccg
    25201 gcggcgggac cagccccaag cagcgccgct tcttcgatcc ggcacgttat cggatcgtcc
    25261 tgttcgacca gcgaggctgc ggcaaaagta agccgcacgc gtctttgaac gacaatacga
    25321 cctggcacct cgtatccgac atcgaaaagt tgaggcagca cctcggcgta gaacgctgga
    25381 tggtgttcgg cggctcgtgg ggcagcacgc tcgcccttgc ctacgcggag acccaccccg
    25441 agcgcgtgac agagctggtg ctgcgcggca tttttctgct caggcggtgg gagctgagct
    25501 ggttctacga gtgcgggacg agccggctgt tccccgaagc gttcgcgaag tatatcgagc
    25561 tgatcccgga gtcggaacgt ggcaacttga tcgaggccta ttaccggcgc ctgaccagca
    25621 atgatccgat tcttcgccaa gaagctgccc ggcgctggag cgtgtgggag gccaccacga
    25681 gccacttgct gccggacgac gagtacattc gcagctgcgc cggcgacgat ttcagcctcg
    25741 cgttcgcgcg catcgagtgt cactacttca tcaacggagg cttcctgaag cacgagaacc
    25801 agctcctgga gcaagtgggg ctaatccggc aaattcccgg ggtaatcgtg caaggtcgct
    25861 atgacgtggt ctgtccggct cagagcgcct gggagctcgc ccaagcctgg ccagaggcac
    25921 gcttgacgat ggtaaccgac gcaggccact cggcgtacga acccggcatc gtgcacgagc
    25981 tcatcacggc caccgacact tttgcatcga aatcggagtg agcgaagatg cgcctgcagc
    26041 gcgccctgag cacgatcaag aagctttgtt cgaaacgcac gcgacgcacc tatcttggac
    26101 gcaaccgcgc gcacatcgag ctgccacagc tctcggagtc agagtgtgaa gcgctcgaaa
    26161 gcgcactgaa gcgcggcttc gcagagctgc ccaaggtacg attcgcggac gtgcaccgcg
    26221 agctttcgcg cgtggtggtc gcgttcgagg acgacgccta tacgctgcga gagctcctgg
    26281 cgatcgtgga gggggccgag cgcgcaatcg gcgtgcacga tgcggtcttc cgggatgagc
    26341 cctgggagca cccggcggac ggcgagtcga tcgaacgcct ggccttggga atggcggtag
    26401 atgccgcgtc gatgctggtc ggcatggggc ttcgagccac gcctttaccg gcgtcacgct
    26461 tggcgggcac tttcgcttcg gtgattgcga tcgtgcagtc gtccccgcgc ctgcgtcgcc
    26521 cgctggatga caagcttgga ccgaaccggg cgagcctggc catgaacaca ggctcggcgc
    26581 ttgcgtacgg cctcgcgcaa cgcccgggca gcgcgttggt ggagctgctt cacaaggcct
    26641 cgaatcatag cgccgtccgc gctcgacagc gcgtgttcga agcgcgcgaa cccgagctgt
    26701 tcgacaagcc gcgcaaggcg ctacctgcgc gcgccccaga ggcgcgcccg cgtcccctgc
    26761 cacgcgggcc aatcgaggag tatgcggatc gcgcttgggt ggtctcgctc ggcggcttcg
    26821 gggtcagctt gctcgcgacc cgcagcgtgc cgcgcgccgt ggccgcgttg ttcggcggcc
    26881 tgccgaagcc ggcgcgccat gggcgagatg ccttttcggc ccacctcgag cgcgacctcg
    26941 cggaacgcgg aatcttggtg attgaccgag aggccgtgcg gcggctcgat cgaatcgact
    27001 gtttggtgct cgagggcagc ctcgtggcgc gggatcgctt ccagatccaa gccgtattgt
    27061 ccgattccaa catcgacgac aacgaggtgc gaggcttggt gcgcgagctg ttcgacgcca
    27121 cgcgcccgat cgctcgcgcc gaagggcgag gttacgtctt ggagccgctc ggaggctccc
    27181 acctcgagcc ccccgcggag ctgcgcaagc gcgcgccgga gctcggcgcg cgcggcggtt
    27241 tggtgctcgc gctctcgcgg gccgaccgca tattggcgct cgcggaggtg gagtggattc
    27301 cgcaaactgg catcgaggag ctgatcgcgg cggcgcggga cgcgcagatg cgcgtggtga
    27361 tcgcctcgaa cgatgagtcc gtgctgcaag gttttgccgc cgacgacacg atcgctgacg
    27421 ccgagggcat gctgcgcggc gtgcgacgct tgcaacgcga aggtcgcgcg gtgtgcgtgg
    27481 tcgcgaccgc gcagagccca ggcctctccg cggcagacgt gagcatcgga ttgctgcgcg
    27541 aaggcgagca gccgccgtgg ggtgcccacg tgatgtgccg gcacgatctg tctgacgtgc
    27601 gcttcttgat ctttgcctgt gtgaccgcac ggcaggtcgc taagcagagc gtcaacgtgg
    27661 cgctcggagc agccaccttg ggggcgttgg tctcagcggg cggggttttg cctctcaccg
    27721 cccgtcgtgt ggcggcggtg gtcagcgggg cttcgctggt atccatggcc aatggggtgc
    27781 ggggcggcat gtcgttggcg cgtcgagctc tgcccccgcc gcgcgatcgt acgccgtggc
    27841 aggccttgga cgcgcaaggt gttctgtatc ggctcaacac ctcgctggag ggtttgactc
    27901 gccagaaagc catcgaacgt cgtgccaagc ccgcgcgcac gcgatctgca ctcggggagt
    27961 tcgtggaggc ggtaagcgac gagctattca atccgctcgc gccgctgctc gccgcggggg
    28021 ctgggctctc cgccgccgta ggctcgctcg gggacgcgac catggtgggc ggcgtggtgg
    28081 tgttgagcgc gctggtcggt ggcgtgcaac gctaccggac cgagcgcgcg atccgtgagc
    28141 tcggcaaagc ttccacgcgc caagcgctcg tgcggcgcgc cgggcagctt caagagatcg
    28201 aagcccgcga gctgtcacgc ggggacatcg tcctgctcgg gcctggtgat gtggtgcccg
    28261 ccgatctccg gatcttggag gcggagcagc tcgagctcga tgcctccagt ttgacgggtg
    28321 agtcgttgcc cgtccgcaaa tcggccaagc cctcgttcga ggcgcacctc gcggaccaaa
    28381 ccagcatgct ctacgagggc atggtggtcg tcgcaggtcg ggccactgcg gtggtggtgg
    28441 cggtgggtga cgagaccgaa gcgcgccgga atgcggtcgc tcaaaagcga gatccaagcc
    28501 gcgggggcgt cgaacggcgc ctccgctctt tgatcgattt gacgggcccc atcgcgctcg
    28561 gcgccggagt cagcctgatc ggctccgggc tgctacgcgg acgcaagatg gaagagctcg
    28621 tcggcagcgg cgtcagcttg gcggtagcct cggtaccaga aggcttgccg ctgctcgcga
    28681 ccgcggcaca gctcgccgct gcctcgcgat tggccaagcg gggagccttg gtgcgcaacg
    28741 tacgcagtat cgaggcgctc ggacgcgtcg acgtgatctg cctcgacaag accggcacgc
    28801 tcaccgaggg tcgaatcgag ctcgccaagc tcagcgacgg ccagcgagcg cgcaagctgg
    28861 ctgaactcgc ggacgcggag cgagacctgc tcgtggcggc gctgcgcgcg agcgaagacg
    28921 accagcgcgc tggcgatccc atcgatcgcg ccttgcacgc ggccgcggga agcttggagc
    28981 tcgggcgaga cgtcttccac aaactgcgca gcgtgccgta cgaagccggt cggggctatc
    29041 acgcatcgct cgggagcgca ggcgaggacc acgttctcac cgtcaaaggt gcgcccgaga
    29101 tcgtgctgcg cttcgcgagt gattggcgcc ggggcggaga gctcgtgccc atcgacgttg
    29161 cgacgctggc ccagctccac cagcacgctg cggatctcgg gcgcgagggg tatcgcgtat
    29221 tggcggtggc ggagcgggtc cgtaggagcg cggtcgagct cgaccccgcc gcccccaaca
    29281 agctcgtatt cctagggttc ctgggtttcc gcgacgcgat ccgcaagacg gcaagacgcg
    29341 cggtgaccga gctctccacg accggcatcc gtacggtgat gttgacgggg gaccatcccg
    29401 cgaccgcgaa gtcggtg
//
LOCUS       DASTKU010000246         5691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_49752,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000246 DASTKU010000000
VERSION     DASTKU010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49752"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..828
                     /locus_tag="VFQ61_09025"
     CDS             382..828
                     /locus_tag="VFQ61_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954635.1"
                     /translation="MPRGIMLSSPDTSAWQFVGRTANSRYFAVEPGILACVPDPKSAD
                     TEGSAKENVRFQELYWTERAEHGTCIVYFDQLVDQTAAARRVYATYPFRSQLATALLG
                     GTALSRAIASFFTGLSQPATRTKFFGSYADAADWCRSVLREEQASP"
     gene            825..1256
                     /locus_tag="VFQ61_09030"
     CDS             825..1256
                     /locus_tag="VFQ61_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954636.1"
                     /translation="MKDSDESLLGGHFEPVGTTDNATFYLVADRVLAIVPFTDTDDND
                     TTSLQSIRFQENFWRERGARGSCIVFMDRVLTQDRRARENYKTAPDASLIAGFALVTS
                     SMLGRAVSSLFVGLSAPAVPTKVFPTMVEAMRWLTSLNQSR"
     gene            1226..2782
                     /locus_tag="VFQ61_09035"
     CDS             1226..2782
                     /locus_tag="VFQ61_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9954637.1"
                     /translation="MADEPKSEPLSDSVVTALVRLAEADFSVRLNRPGDRSRDDVLAF
                     LINSIAEELSTLWEEGKRHQAEQEATVNMVSEGLLALASGRFDWSTARSGDGSPRDVI
                     AFLLNNTAEELSLLVKTREQQAAEVAMLRSARATERLAGMGALAAGAAHELATPLTSA
                     LLFLQEASDAARLEKPPSELLPLISTALEGVERAAVLLDDLRIFVRERPQTLEAVPLD
                     SVIRGAVRLLQHEARSRCKVIVQTKPAAVRANEARLGQVFVNLLKNAIQAIEPGDPEH
                     QLVSITTERVGDRVRVCIRDTGRGMSDEVAARAFEPFFTTKPVNEGTGLGLPVSLATV
                     RELGGELSLRSGSGVGTVVTVELPLAPEATVASVSERDPASDTNPEPTAERPVVIVVD
                     DDPVLSRALSRGLRHEYEVHSACSGEEALRLIAALQGGVDLVLCDVMMPQMTGIELQA
                     RIHQESPELAARFLFISGGGVTEHTRSFLEHLEGYVKKPVSQQQLIAAVRRALRSTRG
                     RHAAREAVIE"
     gene            complement(2979..4241)
                     /locus_tag="VFQ61_09040"
     CDS             complement(2979..4241)
                     /locus_tag="VFQ61_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954638.1"
                     /translation="MRKQLGTRGSLVGIAILAGVTACSKSDGDGSPPASGGQAGSTSG
                     QGGNSSAHGGSTSPQGGNTTSAQGGMTAQSEGGCKADVAGSYVLIDDMETTDHGPIQF
                     TTGISAPLSPGYWYNSGANYAPAAGGGGGVGGATVVDSSDPPQGSFKFSALPKPAATL
                     KCKASEHAAHQSCVLNGLYDTCGVGFEFAQQPDPSAGGMSAGGANAGGTSAGGAGAGG
                     AAAGAGVGGAGAGAAGASAGAAGASAGAAGASAGGTGAGGTASGGAGGGAGVGGAGPS
                     VPMVTVPFNISRYKAVTFWGMTTTPDPTKGSMRVKIQFPDTDTDPRGQVCNGGGPNTS
                     RCYNSYAAYFEFTTTWQQFTVLLDAGTDGVPPKGGIAIDPSWGYQQAKWLPDQVYGIS
                     WQAQKDTAPDAGPPLMTDIWIDDVYFVE"
     gene            complement(4355..>5691)
                     /locus_tag="VFQ61_09045"
     CDS             complement(4355..>5691)
                     /locus_tag="VFQ61_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954639.1"
                     /translation="GNCSNTDMSKINLDASGYVCNNSLGIKGAWYCYSDGSDTGTSCK
                     GSDGKGTGAIPWNSTANAMCMSGTMGTGDGKYAGIGFKLNSGPPGDMTAAGTWDGSKL
                     VGFAVTLTSGPSGKGSGGMVLNLEYPTSNNLDPQTKDAPGVTLPGVGSTEITYNVLFA
                     DSVLANNPKTSTSVDPSKLTDVKLAFFPDSVQHAFDFCIKSIVPLMTAPNPVVPTGNY
                     GPTWNNQKPQAVNGINGYAVQSAPFPTNGNPMTMQVKATAGGVGFSFTAGSGFQAPNN
                     GPGSFPAIISGYGPGHDGIQFYGPYKGGKKISQLTSVKSNWSFTMGSSGDAVYDVWFS
                     NSNTAIPAAELMIWIGNTGKNALGSSSGAAVMGRTPYVGTNGTGEKVISYWIASPGTT
                     SVSNFDLLPYFQDAASHGYAGLSTNSVLLGIQTGFEVYSGNWTTTDYNITIQ"
BASE COUNT         1000 a         1858 c         1802 g         1031 t
ORIGIN      
        1 gagctcggct tcgagcggcc cgcgcacagc gttgggttca gtcagcgatc ggctagctgc
       61 cagccgcgcc tgggcggtct tgacttcgcg caccggcgac gtctcgaacg cggcgattca
      121 gagcgtgacg atttgaaagt cagactcagt gacgagcagg attcgcggcg caaaggctcg
      181 ctcacctcag tcacattctg gaggtgtacc aagggtgcgt tgaaatctgc gtggacatgc
      241 gcacttcgag cgcggcgtac ccgtgcccca gctctcactc gcaggctcga atacccacca
      301 acccctacga tgtggttcgc gaaatctgcg cttggccctg cgcgtttgca ggcttcccgt
      361 gaccttgccg tgcgagaatc catgcctcga ggcatcatgc tcagctcgcc ggatacttcg
      421 gcctggcagt tcgtcggacg caccgcaaac tcgcgttatt tcgcggtaga gcctggcatc
      481 ttggcgtgcg taccggaccc gaagagcgcc gacaccgagg gcagcgcgaa ggagaacgtg
      541 cgcttccaag agctctattg gacagagcga gccgagcacg gcacgtgcat cgtctacttc
      601 gaccagttgg tcgatcagac ggcggcagcg cgacgagtgt acgccaccta cccgtttaga
      661 agccagctcg ccacggcgct cctcggtggc acggccctgt cgcgagcaat cgcctcgttc
      721 tttacgggcc tctctcagcc cgcgacgcgt acgaaattct tcgggtctta cgccgacgcg
      781 gctgattggt gccggagcgt gctgcgggag gagcaggcct cgccgtgaag gactccgacg
      841 agagcctgct cggcggtcac ttcgagcccg tgggcacgac cgacaacgcc accttctacc
      901 tggtcgccga tcgcgtgctc gccatcgtgc cgttcacgga tacggacgac aacgacacca
      961 cgtcactcca gtcgattcgc tttcaagaga atttctggcg cgagcgcggc gcccgcggct
     1021 cttgcatcgt gttcatggat cgcgtgctca ctcaagatcg ccgagcgcgc gagaactaca
     1081 agacggcacc cgatgcctcg ctcatcgccg ggttcgcgct ggtcacgagc agcatgctgg
     1141 ggcgcgccgt aagctccctg ttcgtggggc tcagcgcgcc ggcggtaccc accaaagttt
     1201 tcccgaccat ggtcgaggcg atgagatggc tgacgagcct aaatcagagc cgctgagcga
     1261 ctcggtcgtc acggcgctcg tgcgcctcgc tgaagccgat ttctccgtgc ggctgaaccg
     1321 acctggtgac cgcagccgcg acgacgtctt ggccttcctc atcaacagca tcgccgagga
     1381 gctcagtacc ctctgggagg agggcaagag acatcaagcc gagcaagaag ccaccgtgaa
     1441 catggtctcg gaagggctcc ttgcgctcgc ttccgggcgc ttcgattggt cgacggcccg
     1501 cagcggagac ggttcgccgc gcgacgtcat cgcattcctg ctgaacaata cggcggagga
     1561 gctttcgctc ctggtcaaga cgcgggaaca gcaagcggcc gaggtcgcca tgctgcggtc
     1621 cgcgcgcgcg accgagcgcc tcgccggcat gggcgcgctc gcggcgggcg cggcacacga
     1681 actcgccacg ccgctcacct cggcgctctt gttcttgcaa gaggcctccg acgccgcacg
     1741 cctcgagaaa ccaccaagcg agcttctgcc actgatttcg acggcgctcg aaggcgtcga
     1801 gcgggctgcg gtcctgctcg acgatctcag gatcttcgtg cgcgaacgac cacaaacgct
     1861 cgaagccgtc ccgctcgaca gcgtcatccg cggcgccgtg cgactcctgc agcacgaagc
     1921 tcgcagtcgc tgcaaggtca tcgtccagac caagcccgcg gcggttcgcg ccaacgaagc
     1981 gcggctcggg caggtattcg tgaacctgtt gaagaacgcg attcaagcca tcgaacccgg
     2041 tgatccggag caccagctcg tctcgatcac caccgagcgc gtgggagatc gcgttcgcgt
     2101 ttgcatccgc gacacggggc gcggcatgag tgacgaggtc gcggcgcgag cattcgagcc
     2161 cttcttcacg accaagcccg tcaacgaagg aaccggcctc gggctccccg tttccctcgc
     2221 caccgtgaga gagctcggtg gagagctctc tctccgctcc gggtctggag tcggcaccgt
     2281 cgtcacggtg gaactaccct tggcaccgga agcgacagtg gcgtccgtca gcgagcgcga
     2341 ccccgcatcc gacacgaatc ccgaaccaac tgccgagcga ccggtcgtca tcgtggtcga
     2401 cgacgatcct gtgctctcgc gggccttgag ccgcggcttg agacacgagt acgaagtcca
     2461 cagcgcgtgt tcaggagaag aggcccttcg tctcatcgcc gcgctccagg gcggcgtaga
     2521 cctcgtgctt tgcgacgtga tgatgccgca aatgaccggc atcgagctgc aggctcgtat
     2581 tcatcaagag tcacccgaac tcgccgcgcg cttcctcttc atctcgggcg gcggtgtgac
     2641 ggagcacact cgctccttcc tcgagcacct cgagggctac gtaaaaaaac ccgtcagcca
     2701 gcagcaactc atcgcggccg tacggcgcgc gctgcgaagt acgcgcggac ggcacgcggc
     2761 ccgcgaggcc gtcatcgagt agccgcgttc cgatcgacgc atgagcgaac tacgcggcta
     2821 gctgcgagcc gcgtagtgag gtcgatgcag tgtcggttat cgcaagcgca gggagcgact
     2881 cgctcgttcg ctcttggggc tccaattcgg aaggcgaatt ggacaccaac tcgaaggcgc
     2941 gctggtgaac gggatgcacg gacggatcgc aaaccgtgtc actcgacgaa gtaaacgtcg
     3001 tcgatccaga tgtccgtcat cagcgggggg cccgcatccg gggccgtatc tttctgcgcc
     3061 tgccaactga tgccgtaaac ctgatcagga agccactttg cttgctggta gccccagctg
     3121 gggtcgatgg cgatgccgcc cttgggcggt actccgtccg tacccgcgtc gaggaggacg
     3181 gtgaactgtt gccaggtcgt cgtgaactcg aagtacgcgg cgtagctgtt gtagcaacgc
     3241 gaggtgttgg gaccgccgcc gttgcagacc tggccgcgcg ggtccgtatc tgtatcgggg
     3301 aattggatct tcacgcgcat cgaacccttg gtcgggtcgg gggtcgtcgt cattccccag
     3361 aaggtcacgg ccttgtagcg gctgatattg aagggcacag tgaccatcgg gaccgacggg
     3421 ccagccccgc caacacctgc gccaccaccg gcgccgcccg aggcggtacc acccgcaccg
     3481 gtgccgcccg cgctagcgcc agcagcgccc gcgctagcgc cagcagcgcc cgcgctagca
     3541 ccagcagcgc ccgcacccgc gccacccaca cccgcacccg cggccgcgcc acctgcgccc
     3601 gcgccacctg cgctggtacc accggcgttt gctccccctg cgctcatgcc gcccgcggac
     3661 gggtcgggct gctgcgcaaa ctcgaagccg acaccgcacg tgtcgtaaag cccgttgagc
     3721 acgcaggact gatgggctgc gtgctcgctg gccttgcatt tcagcgtggc ggcaggcttt
     3781 ggtagcgcgc tgaatttgaa cgaaccttgc ggcggatccg aggagtcgac cacggtcgcg
     3841 cccccaactc caccgccacc gccagcagca ggcgcgtaat tcgcgccgct attgtaccaa
     3901 tagcccggag agagcggtgc gctgatgccc gtggtgaact ggatcggacc gtggtcggtc
     3961 gtctccatgt cgtcgatgag cacgtaggag ccggcgacgt ccgctttgca gccaccttcg
     4021 ctctgcgccg tcatgccgcc ttgcgccgac gtcgtgttgc cgccctgagg ggatgtcgac
     4081 ccgccatgcg cggacgagtt gccaccttgt ccggacgtac tccccgcctg accaccactg
     4141 gccgggggag agccgtcgcc gtcgctcttc gagcaagcgg tcactccggc gaggattgca
     4201 atgccaacga gcgaaccacg tgtaccaagc tgcttgcgca tgaccgctac tccaactccg
     4261 aattcaagac cgacgctacc aaatttgcgt caaatacgcg aaaccactcc gacgcgggaa
     4321 gcgccggagt ggctcgtcta ggtacgcagg gcaatcactg aatggtgatg ttgtagtcgg
     4381 tcgtggtcca gttaccgctg tagacctcga agccggtctg aatgccgagc agaaccgagt
     4441 tcgtcgagag gccggcgtaa ccgtggcttg ccgcgtcctg gaagtacgga agcaagtcga
     4501 agttcgatac gctcgtagta cccggcgagg caatccagta gctgatgacc ttctcaccgg
     4561 tgccgttcgt gccgacgtag ggcgtcctcc ccatgacggc ggcgccagac gaactgccca
     4621 acgcgttctt cccggtgttg ccgatccaaa tcatgagctc cgcggcggga atcgcggtgt
     4681 tggagttgct gaaccagacg tcgtaaacgg cgtcacccga agagcccatg gtgaagctcc
     4741 agttgctctt caccgacgtg agttggctga tcttcttgcc gcccttgtag gggccgtaaa
     4801 actggatgcc gtcgtgaccg gggccgtacc cgctgatgat ggctgggaag gagccgggac
     4861 cgttgttcgg agcttggaaa cccgaaccgg cagtgaacga gaatccaacg ccacccgcag
     4921 tggctttgac ctgcatcgtc atggggttgc cgttcgtcgg gaagggcgcg ctctggacgg
     4981 cgtagccgtt gattccgttc accgcttgag gcttctggtt gttccacgtc gggccgtagt
     5041 tgccagtggg gacgaccgga ttcggagcag tcatcagagg aacgatgctc ttgatgcaga
     5101 agtcaaatgc gtgttggacg ctgtccggga agaaggcgag cttcacgtct gtcagtttgc
     5161 tcggatcgac gctggtgctg gtcttcggat tgttggcaag gaccgagtcg gcgaaaagca
     5221 cgttgtaggt aatctcggtg ctccccacgc ccggcagcgt gacgccgggg gcgtccttgg
     5281 tctgcggatc gaggttgttg ctcgtcgggt actcgaggtt cagaaccatg ccgccgctgc
     5341 ccttgccgct cgggccggag gtcaacgtca cggcaaagcc aacgagcttg ctgccgtccc
     5401 atgtaccggc ggcggtcatg tctccgggcg gacccgagtt gagcttgaag ccgatgcccg
     5461 cgtacttgcc gtcgccagtc cccatcgtgc cgctcatgca catggcgttc gcggtcgagt
     5521 tccatgggat cgcgccggtg cccttgccgt cgctgccctt gcaggaagtg cccgtgtcgg
     5581 acccgtcgct gtagcagtac caagcgccct tgataccgag gctgttgttg cagacgtaac
     5641 ccgatgcatc gaggttgatc ttgctcatgt cggtgttgct gcagttgcca c
//
LOCUS       DASTKU010000247         7279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1834152, whole genome shotgun sequence.
ACCESSION   DASTKU010000247 DASTKU010000000
VERSION     DASTKU010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834152"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..928
                     /locus_tag="VFQ61_09050"
     CDS             263..928
                     /locus_tag="VFQ61_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9954640.1"
                     /translation="MKSLLSHGHRTGYLRFNIGHAVRAPKVDVDVDHRSLTEVQIALM
                     IQAAATALRAERSRTTPRPRYVRAALTRLFFARFLYYSGARVAEAVAVRWKDLRPRPD
                     GDVQLSVLGKGRKRRSFPLPGGVVRQLEAEYKRNAAPDSLIFGFGVRRAQTIIRELAV
                     LSGLEQAVSPHWYRHAAATHALDRGAPIHVVAQTLGHASLATTSRYAHKRADGAARYL
                     PRI"
     gene            1845..3104
                     /locus_tag="VFQ61_09055"
     CDS             1845..3104
                     /locus_tag="VFQ61_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954641.1"
                     /translation="MVLRPDGLLVLTRAGKTWRGFVEVKVGSSPMEREQIEKYLQLAR
                     QEDVSALITISNEAAINGGPPVAGLNKGLLKGLTVIHLSWERLLSEARLLRGQDRVED
                     ADQQWILDEWIQYVSDPSSKIIDPPSLGPRFGDLLTAAKEGNLAGAAPAVKDVCGYWD
                     GFLQKVADRLRADLHVEVSAAMTAAERQDNELRVRNLQVVAVNEGRLTGSLKVKNAAS
                     DITIDVLLSARAVRFGVEVKAPSEGRQLTRLRWMTKQLAKAPPEALLHVLWDQKKLKS
                     QARMADALGDVACLTRDISLQPVPADALPRTFSIEWTQDLQKGKGKAVVLDGIMADLE
                     KFYGLVVEGIVAFVPKAPKLTSAVEAAPAPEGLPEPPATPVPELDAPAESAVLTHSAA
                     PGAAPDVEEQGNRGPRPSTPPGRAANE"
     gene            3336..5201
                     /locus_tag="VFQ61_09060"
     CDS             3336..5201
                     /locus_tag="VFQ61_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954642.1"
                     /translation="MRTKNRFIALVGYLNIIAATAAVLCLLAAVAVVRRRRIYVDPIV
                     ILRAGAFEQLVKARALQSAQAQVQVRNVALCAPIEAGRSVPVEAHRGMQAAAAEWSAA
                     VADGVVPALGVWAQWESVDHHVFQAFSHLAGEQIDGMADLLRVVDTKEYAIQSAGLLN
                     KLLGHVGEWHARDHLASAGFSVSMPEATNAPGLDLWADGNAVNVKTVKDAAAAAYQHF
                     GDYPDIPIIVPADAANIPSDALHFDPSSGFDSAALAGGDHLVVVDHALSHADVLDQSQ
                     NALDVLGDPGPHLHFPWITAALSGVREARLLVKGHTDLARAAKNVAVDTAAVGGGGAV
                     GMKVGALVGAALGPVGAVVGGIAGGLLGAIGGRSAANTVKRAPFEAARTEYEVALATY
                     HATEAKLVAEAGATWERAQQEEGGALNTELRRLQGEFDSVLAEQARRLHRTAILEKNQ
                     AQELLLKAGTTLASVCDGNRVALHALLPRWLWPWAGLLVPAHAQRLVQHGREHTRWQR
                     EVERVMRVWTGSASDTSACFDLLMATAGGEAAAEAHLAKAALARQRVVSAAGQHHQRL
                     LAQATQSRAVTVERLRQRWQKISAHVSLSLEEVVTTLRAKGADLHREMQKNGMTG"
     gene            5512..5823
                     /locus_tag="VFQ61_09065"
     CDS             5512..5823
                     /locus_tag="VFQ61_09065"
                     /inference="COORDINATES: protein motif:HMM:NF016227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding protein YggL"
                     /protein_id="HET9954643.1"
                     /translation="MRRRLRKKLHRAEFTELGFEVVADLDLGLDQEQRYAFLDRFIDA
                     VEARQLGFGGGGSTPFAGFVARLGRASASAADREALETFLASEPAVVRHQVGPLVDAW
                     R"
     gene            5835..6176
                     /locus_tag="VFQ61_09070"
     CDS             5835..6176
                     /locus_tag="VFQ61_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954644.1"
                     /translation="MKKRPLLSIGQEFTHPGTGHVHRVTDVGSRTFLTIDTTSGTIAA
                     RDSETGETTTRVVTGNEWQSWLSGPPYAVAENVWDEYDQEVLRDVEGINWVDVSTSEE
                     MNAPRGGSQRR"
     gene            6292..6951
                     /locus_tag="VFQ61_09075"
     CDS             6292..6951
                     /locus_tag="VFQ61_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954645.1"
                     /translation="MGHVTHEASWCTIDVDTEAGKVVVLQRWQYEWLLESDRMTRWTL
                     AEKQAFHARAEREIWNAWSNRAHLKVEGSSDFAKRFRGREIPVFMDIRWVLGKPHFTV
                     RVTKVAEKVFIQSRVPWDKHTIHLDTNDFVTRTKCFGPNKTQCVSQVPVAHEFGHSLG
                     PVSGGMANRDEYRTKSEHYGDINSIMHRGNQLRARHFEQLMTELFELIPETSFSVGRV
                     Q"
BASE COUNT         1394 a         2249 c         2414 g         1222 t
ORIGIN      
        1 ggccgctcgc cttccaggcc ttgacaatct cggtccattc atctcgggag cgggaggggc
       61 cccgcctgcg tttgtttgcc atcgccgggc gctagcccgc gcgtcgccca ccgtgaagga
      121 cgtggtccgc ggaacgggta ccttcttgcg gtggctcacg agtcatgggc acagccttcg
      181 tacactgacc accgaacacc tggtttcctg gcgcgaccag ctgaagggag cccccgctac
      241 caggggaaat cggctcgccg tcgtgaagag cctcctgagc cacgggcacc gaacaggtta
      301 tcttcgtttc aacatcgggc acgcggtgcg agcgcccaag gtggacgtcg acgttgacca
      361 ccggtctttg accgaggtcc agatcgcgct gatgattcaa gcggcggcga cggctctacg
      421 agccgagcgg tcgcgaacaa cgcctcgccc ccgatatgtc cgggcggcac tgacccgact
      481 gttcttcgcc cgtttccttt actatagtgg ggcgagggtg gcggaggccg tcgcggtcag
      541 atggaaggat ctgcgcccgc gccccgatgg cgacgtccag ctcagcgtgc tcggcaaggg
      601 gcgaaagcgc cggtcattcc cgcttccggg cggtgttgtg aggcagctcg aagcggagta
      661 caagcggaac gccgcccccg actcgctcat cttcgggttc ggcgtgcgca gggcgcagac
      721 gatcatccgc gaactagcgg tcctctccgg ccttgagcaa gccgtctccc cgcactggta
      781 tcgtcatgcc gcagccacgc acgcactcga tcgaggagcc ccgatccacg tggtggcgca
      841 aaccctcggg catgcgagct tagccacgac gagccgctac gcacataaac gagccgacgg
      901 cgctgctcgg tacctgccga ggatctaggt ggcagtcagc gctgctgctc ggcgtcgcct
      961 ttcccccatt ggtgccgagc cagacgttaa cttggaagca gcgaattccg cgtttgtcca
     1021 gccacactcg cggggcatgc ggaggggtcc tgtcggcgaa ccagccccag ctcactccgt
     1081 cacgatcgac atgggcccag ccgacgagac gaatcgacga aacacacgat gcgcggcgct
     1141 gtcgggcatc cgagtcactt cgaatcggtg gaaagcgcgt gacggcgcgc gcggcatcta
     1201 gcgcgtcaca aggtgatcga ccgtcggtca gcgtcgaggc gcccgctgac attcagcgag
     1261 caagaacctg ccggctatct ccgaagcggc ctcggttctc gaagagaacc ctcgggtgga
     1321 agggcgtacc cgctgcgcat cgcaaccggt tttctggccg ggaagctgcc ccccctcaca
     1381 catgcagccg aatcgatttc ccaaaggaat tggccgcgtc ttcagaaaat cggggggcgg
     1441 aaattatggg cgaactggct gcttgcgggg accccacgcc gtagccctgg tgggacactt
     1501 gcacacccga aagctcaagg tccacggtca agaaccgtct tgacgatcag cctgacggcg
     1561 agcccccgct cgagcgagcg gtccgttgag aaggcgacac tggagcagga agcatgccct
     1621 tcgaagatac cgaccaggcg aaagcacatc gagtccggct cttcccgacg atcaggatcg
     1681 gctctgtcgc ggaggcggag acgagagcga cggctgcgct gctggccacg ctgcgggccg
     1741 tctcggagtt cgggcgggtc atcgtcaagc tcgcgggtgg ccctgccggg aagcccgact
     1801 gcttcaccga ggtaccgtac gaggatcgtt cccagtttcc ggccgtggtg ttgcgcccgg
     1861 acggccttct cgtcctcacg cgcgccggga agacctggcg aggcttcgtg gaagtgaagg
     1921 tggggagcag ccccatggag cgggaacaga tcgaaaagta tctccagctc gcgcggcaag
     1981 aagatgtcag cgcgctgatc acgatctcga acgaggcggc gatcaatggc ggacctccgg
     2041 ttgccggtct caacaagggt ctcctcaagg gccttacggt gattcatctc tcctgggaga
     2101 ggctcctcag cgaggctcgg cttctgcgcg gccaggatcg cgttgaagac gcggaccagc
     2161 aatggatact cgacgagtgg atccagtacg tgtcggatcc ctcatcaaaa atcatcgacc
     2221 cgcccagtct gggtccgcgc ttcggggacc tcctcacggc cgccaaggag ggcaacctcg
     2281 cgggcgccgc tcccgcggtg aaagacgtct gtggttactg ggacggcttc ctgcagaagg
     2341 tagctgaccg gctccgcgcg gatcttcatg tcgaggtgag cgctgcgatg acggccgcgg
     2401 agagacaaga caacgagctg cgggtccgga accttcaggt cgtggcggtg aacgaaggcc
     2461 ggctcacagg gagcttaaaa gtcaagaacg ccgcctccga catcaccatc gacgttctgc
     2521 tctctgccag ggcggtacga ttcggcgtcg aggtcaaggc gccgtcggaa ggccgacagt
     2581 tgacgcggct caggtggatg acaaagcagc tcgcaaaggc tcccccagaa gctcttctgc
     2641 acgttctctg ggaccagaag aagctgaaga gccaggcacg gatggccgac gcccttggcg
     2701 atgttgcgtg cttgacgagg gacatcagcc tgcagccggt ccccgccgac gcgctgccac
     2761 gaacgttctc gattgagtgg acgcaggacc ttcagaaggg gaagggtaaa gctgtcgtgc
     2821 tcgacggaat catggcggat ctggagaagt tctacggtct ggtggtcgag gggatcgtcg
     2881 cgttcgttcc gaaggcgccg aagttgacct cagccgtgga ggctgcgccc gcaccagaag
     2941 ggctccctga accacctgct acacccgttc cggagcttga tgcccctgcc gagtcggccg
     3001 tgctcacgca cagtgccgct cccggggccg caccagacgt cgaggaacaa gggaaccgag
     3061 gtccaagacc ctcgacacca cccggccgcg cagcaaacga gtaggctcgc ttctgctagc
     3121 tctgggtatc tcgacttgct ctcgccggga gctgatgcgc cctggcttcc tgatgcatat
     3181 tcgcttcacc gttgcccgcg cgccaggcga tcggcaaacg gcgcccatgg ccgtgccatg
     3241 gatcgatggg gccggcgacg cggaagtcca acagtaggcg ccaattatgc gcgcaacggt
     3301 gttcgaacgg ggtgcaatca cccgcaagct caagcgtgcg gaccaagaac cgtttcatcg
     3361 ctctggtggg ctatctcaac atcatcgctg cgacggcagc cgtactctgc cttcttgccg
     3421 ctgtagctgt ggtgcgacgg cgtagaatct acgtcgaccc aatcgtgata cttcgtgccg
     3481 gggcgttcga gcagcttgtc aaagcccggg cgctgcagtc cgcgcaagcg caggtgcagg
     3541 tgcgcaacgt cgccctgtgc gcacctatcg aggcagggcg atccgtgccc gtcgaagcgc
     3601 accgtgggat gcaggccgcg gcggccgaat ggagcgccgc tgtcgcggat ggcgtcgtcc
     3661 cggcgcttgg agtgtgggcg caatgggagt cagtcgatca ccatgtgttt caggcgtttt
     3721 cgcaccttgc cggtgagcaa atcgatggga tggcagacct gctgcgagtc gtggacacga
     3781 aagaatatgc aattcaatct gcaggccttc taaacaagct cctcggacac gtcggcgaat
     3841 ggcacgctcg ggaccatctg gccagcgctg gcttctccgt ctcgatgcct gaggccacga
     3901 atgctccggg cctagacctc tgggctgatg gcaacgccgt caatgtcaag accgtcaagg
     3961 acgcagcagc ggcagcctac cagcacttcg gcgactaccc tgacataccc atcatcgtgc
     4021 ctgcggacgc agcgaacatt ccatcagacg cgctgcactt cgatccgagt tccgggttcg
     4081 actctgccgc gctcgcgggt ggtgatcacc tcgtcgttgt cgaccacgct ctgtcccatg
     4141 ccgatgtgct cgatcagtcg cagaacgctc ttgatgtcct gggggatccg ggaccacatc
     4201 tccattttcc gtggatcact gcggcattga gtggggttcg ggaggccagg cttctagtga
     4261 aggggcatac ggaccttgct cgtgccgcga agaacgttgc tgtcgacacc gcagctgttg
     4321 gtggcggggg cgctgtcggg atgaaagtcg gggcgctggt cggcgcagcc ttgggccctg
     4381 ttggagccgt cgtcggtggt attgcgggcg gtctgctggg tgcaatcggg ggaagaagcg
     4441 cggcgaacac ggtaaagcgg gctccctttg aagctgcaag gacagagtac gaggtcgcgc
     4501 tggcgacata ccacgcgacg gaagccaagc tggttgccga ggccggggca acttgggaac
     4561 gagcgcagca ggaggaaggt ggtgcactca atactgaact gcgccgtctt cagggcgagt
     4621 tcgacagcgt acttgccgag caggcgcgcc gcctccaccg gacggcgatc cttgaaaaga
     4681 accaggcaca ggaactactt ttgaaagccg ggaccaccct ggcatcggtc tgcgacggga
     4741 atcgcgtcgc cttgcacgcg ctactcccac ggtggctgtg gccatgggcc ggcttgctcg
     4801 ttccagccca tgcgcaacgc cttgtccagc acgggcgaga acatacccgc tggcaacgcg
     4861 aagtcgaaag ggtgatgcgc gtgtggacag ggtcggcttc cgatacgagt gcatgcttcg
     4921 accttttgat ggcgacggca ggtggcgagg cggccgccga ggcgcatctc gccaaggcgg
     4981 cgctcgctcg tcagcgggta gttagcgccg caggccagca tcaccagcgg cttctcgcgc
     5041 aggcgaccca gtcccgagca gtcacggttg aacggctacg tcagcggtgg caaaagatca
     5101 gtgcccacgt cagcctctcg ctcgaagaag tcgtcaccac ccttcgagca aagggggccg
     5161 atctccaccg cgaaatgcaa aagaacggga tgacggggta gctgtagtgg cagtcccgct
     5221 cggttcaacg tacgaggaag cacgatgaac cggcgttgac ctgcttagac ccgcgctggc
     5281 cgccggttta ggcattccgg gccacgtcga caatcgcgcg agcaggttgg ggtcggagca
     5341 tcgctcaaac ggtgcaccgg tgatcgcgtc gcagagcggg ccattccgag cggcgcaggg
     5401 acgatgagcc cgccgttcag ccaaggaagg agccgcaaat gcgggcgatt ccaggggcct
     5461 gggagcgtca tttcccaagc gattcagacg cttggtggta gcctcgcacc gatgcgtcga
     5521 cgactccgca agaagctgca tcgggccgag ttcacagagc tggggtttga agtcgtggcg
     5581 gaccttgacc tgggactcga tcaggagcag cggtacgcct ttctcgaccg attcatcgac
     5641 gccgtcgagg cgaggcagct cgggttcggt ggaggaggca gcaccccctt cgccgggttc
     5701 gtcgcgcgcc tcgggcgagc gagcgcgagc gctgccgatc gggaggcact ggagaccttt
     5761 ctagcgagcg agcccgccgt ggtccggcac caggtggggc cgctcgtgga cgcctggcgt
     5821 tgaagcgaac tacgatgaag aagcgccctc tgctcagcat cggacaggaa ttcactcacc
     5881 cgggcaccgg ccacgtccac cgggtaacgg acgtcggaag ccgaacgttc ctgaccatcg
     5941 acacgacgag cggcacgatc gccgcccgcg attcagagac cggcgaaacg acgacgcggg
     6001 tcgttacggg caacgaatgg caaagctggc tctccgggcc gccgtacgcc gtcgccgaga
     6061 acgtctggga tgagtacgac caggaggttc tccgcgacgt cgagggcatc aactgggtcg
     6121 acgtttcgac ttcagaggaa atgaacgccc cgcggggcgg gagtcagcgt cgatgagcgc
     6181 gggggcacga acggcggttt caggcattca ccggtggagc ggcgtgccaa ggggccctag
     6241 agcgtttcag gcccatgtgg cggcgcgcaa cgtcgggaac gagtgtagct gatgggccac
     6301 gtcacgcacg aggcatcgtg gtgcaccatc gacgtcgaca ccgaggctgg caaggtcgtg
     6361 gtgctccagc ggtggcaata cgagtggctg ctcgaatcgg atcgtatgac gcggtggacg
     6421 ctcgctgaga agcaggcctt tcacgcgcgc gcagagcgtg agatctggaa cgcctggagc
     6481 aaccgggccc acctgaaagt cgagggcagc tccgacttcg ccaagcgctt ccgcgggcgc
     6541 gagatcccgg tcttcatgga catccggtgg gtgctcggca agccgcattt cacggtccga
     6601 gttaccaagg tcgccgagaa ggtgttcatc caaagcagag tgccttggga caagcacacg
     6661 atccacctgg acacgaacga cttcgtgacg cgcacgaaat gcttcggccc caacaagact
     6721 caatgcgtga gtcaggtccc ggtggcgcac gagttcgggc acagcctagg ccccgtctcg
     6781 gggggcatgg cgaaccggga cgagtaccga acgaagagcg agcactacgg ggatatcaac
     6841 agcatcatgc atcggggcaa tcagctgcgc gcgcgacact tcgagcagct aatgaccgag
     6901 ctgttcgagc tgatcccaga gacgtccttc agcgtggggc gcgtgcaatg acgagtcgcg
     6961 cttggatccc atgcttgatc agcttgtgct gctgcaaggc tgatgacggt gcgaagtccc
     7021 cgcagccttc gcctgcaatc accgcgatcg agacaccgaa ggctgcagca gcgcctgagc
     7081 ccgagaagct cccagcaacg tcatgcgagg aggggctcga tttcaaactc gccggcacgc
     7141 tgcagctggc cacatagatc cgcagaaatc tgacccaaga ggtgggccag gactggtcgt
     7201 ataggccaga cggggcggat cgaggcgagc gaaacagaag agggcctggg ttgcagccca
     7261 gaccctccga tcagcgagc
//
LOCUS       DASTKU010000248         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1388326, whole genome shotgun sequence.
ACCESSION   DASTKU010000248 DASTKU010000000
VERSION     DASTKU010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388326"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VFQ61_09080"
     CDS             complement(<1..517)
                     /locus_tag="VFQ61_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954646.1"
                     /translation="MLLRSPSSLGALIWITALAAACGEGGSPPLGAAGASPGAAGMGS
                     APGASGGASGGIGAAGTGAATGGSPGIGGAGGSSGGIASTGGAPVSTGGASAATGSRS
                     STGGAATGGSAAGGSSAAGGSPSMGGSSSSGGRNPSGGHSGGGGMSAGGTAGAPSSGG
                     ASTAGSGGAANG"
     gene            768..1733
                     /locus_tag="VFQ61_09085"
     CDS             768..1733
                     /locus_tag="VFQ61_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9954647.1"
                     /translation="MGSLKHASSPKSFTLRGRTLVGRSSRADLKLSGDRTSSEHATIF
                     WTGAHWVLRDLKSRNGTRVNQMLVSGRDCPLSVGDEILFGDPNERWLWADAAAPEPSA
                     TREDGAVVLAQNGILFLPDHESPQATVHERDGKWELDASGAIREVKDAESISVGRHQF
                     TLSLPEIEPEYARTHTLVASMRVAAARVKFKVSLDEEHVQVSFAANDVQKQLSARAIN
                     YLLLELARARSEDQSANLPEDDAGWIYTDALAERLGVAPEAINVDVYRLRHALAKLQM
                     FSDPEEVIQRRRSTAQLRLGCPGIEIEQAGRSELSKRGSRKPRAR"
     gene            complement(1764..>2872)
                     /locus_tag="VFQ61_09090"
     CDS             complement(1764..>2872)
                     /locus_tag="VFQ61_09090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9954648.1"
                     /translation="FRSELASGGARAASLLCFPLRRMQTTRAIVYLENRLSREAFTAR
                     RIKALELLSSQAVISLENARLYDELEQKVEKRTRELLDKNAELGRALQQVTEMQRQLV
                     TQEKLAALGALTAGIAHEIKNPLNFITNFADVSARLSEEVEQVLEPELDRIAAPVQSE
                     VNAVLGELRVAVQKIAEHGARATAIINGMAAHARGGSGPREPTQLNALLAQSVELAAH
                     AVGSPRLGELQIRSDYDPQVEPLEVVPQDISRVFVNLINNARYAMEQKHTRAGAGYAP
                     VLNVVTRDLPDAVEIRIEDNGIGIPQAVLDKIFVPFFTTKAAGQGTGLGLSISHEIIV
                     RGHGGELRVESVEGQWTQFIIRLPKRVPKSERWA"
BASE COUNT          503 a          946 c          913 g          510 t
ORIGIN      
        1 cgccgttcgc ggctcccccg ctgccagcgg tcgaggctcc cccggaactc ggagcaccag
       61 ctgtgccgcc ggcggacatg ccaccgccgc cgctgtggcc accgctagga ttgcgaccac
      121 cgctcgagct gctcccaccc atgcttggtg acccgcccgc tgcagaagat ccgcctgcgg
      181 ccgagcctcc cgtcgctgca cctccagtgg aggaacggga tccggtcgcc gcactcgctc
      241 cgccagtcga aaccggcgcg ccacccgtcg aggcaattcc gccactcgag ccgccagcgc
      301 cgcctatgcc ggggctgcca cctgtcgcgg cgccggtgcc cgctgcgcca ataccgccac
      361 tcgccccgcc agacgcgccc ggcgccgagc ccatgcccgc agcgccgggt gaagctccgg
      421 ctgccccgag aggcgggctg ccgccttcac cacacgccgc ggcgagcgca gtaatccaga
      481 tcagagcacc caatgaggac ggagaccgca acagcatgag cttaccttct gaggagcgcc
      541 tcagcgtaca caccagtgtt ccgaggtgca gcgcacgggt cggcgaacga cgatttcgcg
      601 gtcagatgcg agtcagcacg gaattctcca tgcccttacg agttctgggc gaccggagtg
      661 agaggccgtc acccatggtc cccagggtcc gcgaagtggg gccacgcgct gttcaggcgg
      721 gtgagctcgg tggagccgcg ctccgcaaac tgtcgtagca tgcgtacatg ggcagtttga
      781 agcacgcgag ctctcccaag tcgttcaccc tccggggtcg aactttggtg ggacggtcct
      841 cgcgagccga cctcaagctc agcggtgacc gcacctcgag tgaacacgcc acgatctttt
      901 ggacgggcgc gcattgggtg cttcgggatt tgaaaagccg caacggcacg cgcgtgaacc
      961 agatgctggt cagcggccga gattgccctc tgtcggttgg ggacgagatt ctattcggag
     1021 atccgaacga gcgctggctc tgggcggacg cggccgctcc cgagccgtct gctacgcgtg
     1081 aagatggggc cgtggtcctg gcgcaaaacg gcattctctt tttgccagat cacgagtcgc
     1141 cgcaggctac cgtgcacgag cgcgacggaa agtgggagct cgatgcttcg ggcgcaattc
     1201 gtgaggtgaa ggacgcagag tcgatcagcg tcgggcggca ccaattcacg ttgagtttgc
     1261 ccgagatcga gcccgagtac gcgcgaaccc ataccctggt cgcgagcatg cgggtggcgg
     1321 ccgcccgcgt gaagttcaaa gtcagcttgg acgaggagca cgtccaagtg agttttgcag
     1381 cgaatgacgt gcagaagcag ctgagcgcgc gcgccatcaa ttatctgttg ctcgagctgg
     1441 cccgggcgcg aagcgaggac cagtcggcaa atttgccaga agacgacgct ggttggattt
     1501 acacggacgc gctcgcggag cgcttgggag ttgcgccgga agccatcaac gtggacgtct
     1561 accgcttgcg tcacgccctg gccaagctgc aaatgttctc cgacccggaa gaggtcatcc
     1621 aacggcgccg cagcacggct caattgcggc tcggctgccc cggaatcgag atcgagcagg
     1681 ccggcagatc ggagctttcc aaacgcggct cacgcaaacc gcgggcgcgg tgagaggtcg
     1741 atttcccggc ggcgtgtcag cgttcaggcc caacgctcac tcttgggtac ccgttttggc
     1801 aggcggatga tgaactgagt ccactgccct tcgacgctct cgacccgcaa ctcgccgcca
     1861 tggccgcgta cgatgatttc gtgactgatg gagaggccta gcccggtgcc ttggccggcg
     1921 gccttggtcg tgaagaaagg gacgaagatc ttgtcgagca cggcctgggg aataccgatg
     1981 ccattgtctt cgatccgaat ttcgaccgcg tcgggcagat cgcgcgtcac gacgttcaga
     2041 actggcgcat agcccgctcc ggcgcgggtg tgtttttgtt ccatggcgta gcgcgcgttg
     2101 ttgatcagat tgacgaagac gcgactgatg tcttgcggca ccacctccag cggttccact
     2161 tgcggatcgt agtcacttcg aatctggagt tcgcctagcc gcggtgaacc gaccgcgtgc
     2221 gccgcgagtt ctacgctctg cgcgagcagc gcgttcaatt gtgtcggttc cctcggcccg
     2281 cttccacccc gcgcgtgtgc ggccatgccg ttgatgatcg cagtggctcg cgccccgtgc
     2341 tccgcgatct tctggacggc tacccgcagc tcacccaaga ccgcgttgac ttcgctttgc
     2401 accggcgcgg cgatgcggtc tagctctggc tcgagaacct gctccacttc ctccgacaga
     2461 cgagccgaaa catccgcgaa gttggtgatg aagttcagcg ggttcttgat ctcgtgggcg
     2521 atgccggccg tcaaagcgcc aagcgccgca agcttctcct gcgtaacgag ctggcgctgc
     2581 atctccgtga cttgctgcag cgcccgccca agttcggcgt tcttatccag cagctcgcgc
     2641 gtgcgttttt ccaccttttg ctcgagctcg tcgtacaaac gtgcgttctc gagcgagatc
     2701 acggcctggc tggacagcaa ctccaaagcc ttgatgcgcc gagccgtaaa cgcctcacgc
     2761 gagaggcggt tctccaggta cacgatcgcc cgcgtggttt gcatccgccg caaagggaag
     2821 cacagcaaag aagcggcgcg cgccccgccc gaggcgagct cgcttcgaaa gc
//
LOCUS       DASTKU010000249        12465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1164772, whole genome shotgun sequence.
ACCESSION   DASTKU010000249 DASTKU010000000
VERSION     DASTKU010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12465
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164772"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFQ61_09095"
     CDS             complement(<1..294)
                     /locus_tag="VFQ61_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954649.1"
                     /translation="MSTTPPISGKIELSGRKEHAMHALERAVAASERGFSDRPVTLIS
                     AEDLACLSRPDVRRALDRVMLGQHADLGLDALLVSGVLEVLLPEVKAMVGFGDG"
     gene            505..4896
                     /locus_tag="VFQ61_09100"
     CDS             505..4896
                     /locus_tag="VFQ61_09100"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HET9954650.1"
                     /translation="MLLRPVLRPGRRVDLGRWFARFACVVFGLLGALPLLVEGVLRLH
                     SVQSYVDRQTAQLLSRELGLVASYEVTAHLIPLRLTVERLRVPASDGGSPALEVEKVI
                     VTPRIFSLLAGRLDVGNVEIESPRARLVLRDGKLTNVKYTLKPSKGSPKPSKEAPFSA
                     LSVSEARVSLDLDGLQIESGPTDIDVFAESGPAFEVMLSGAATNLEWRHPPKRGTLPI
                     EVDEDRLCRLDLRARIEGRQFLVRRLSALGVSDLDPAPGSTPSCDVGDGAVGRVALRL
                     SQLRVSLPERGAPLVSGHVVVRGPLALTNRFVATQPLAGWAGFAGDVRYDGRTRLPLI
                     QGHVSGAGIAFERYRLASSLSVDVDVRGDVVRVPRYEMDFADGHVVLENARIDPFAEG
                     VPLSVSRVDSRALTFPGLMRDLGVTPNTIVAWNMIDTLVSNLKGTLSPLKFDADLAAD
                     TKDFEVTDRAFHDPHRKHMVGVKSAEIRGKIGVRPNALIFYDTRAEFGKSSMQVKLVS
                     IGFDNRLELEIGKGSRLHLADVSPIVDIPVAGESELDASMSGAANDPVLLGNLSVKGF
                     EFGGFPLGDIKSAKAKFRPLFLELSDVLSTKGSSDFTVPFARIDFGTRASVAVQAQAR
                     SPKLDLRDFFKMWHFDEDPRFEPIRGTSAVDASIRYVLGGPEDRCGGGVLRVQGQLKT
                     RELELYEEHYDGGESSFDFLWHDREAAYRGVIFNAPDFTLNKGSGRIAGELTMQTGAK
                     VAGKVVATGIPVGKIDALPTLLRAAEGTVNATGTVSGTLDALVADINANVSAIQLGLG
                     SLPASTFRVHLQPTIQPETVIGKTRCGEPISGPFERAQYDRDLESGVFYTDGELFGGQ
                     IVLERLSVTRQRDKTVRGKVHFRDLGLGALLQLGRSTATPSTPMSGLASGTLSLDELR
                     MADPKAARGTLALKTLAVERQGYRVQLLDGAQPFRIGGRAVDLPSAALQVETPEGQKA
                     VFDLSGRVSNLGQNPQLDAGVQMRPTALASLVGLVPRVERANGVLSGRFRLTGALNAP
                     STTGTLDLQRGELALRGLPTPLSDIDVGVAFDHGELRVERGSARLGSGSVSFSGSAPL
                     RGFSIGEGRFRAVAHEVSLPLKEGIHTNLDADLQISIRPAGDSETRPLPRVTGNVMLR
                     SFEYKRPITIAADLNTLAQRGKRTAIEDYDSRDDAVEFDVNLRSGRPLKIQNDIVEAE
                     LVLSEDGLELAGTNGRFGLRGNVRLKQGGRVTLRRSEFEIQEGSVRFDDLTRIAPLVD
                     VSAVTEYRRYSDAPGTTSQSTTNTATLTGTSTTVTGGRWTIRMHAYGDADNLKVDLSS
                     TPALAQDDIFLLLTVGLTRAELDQAQSASVGGSVALEALGTLTGADRAVKKVVPIIDE
                     FRFGSAYSSRTGRTEPTVTVGKRLTERVRANITSGLSESREVRSNVEWRLSPKVSVEG
                     SYDNVNDISSSALGNLGADVRWRLEFE"
     gene            complement(4926..5672)
                     /locus_tag="VFQ61_09105"
     CDS             complement(4926..5672)
                     /locus_tag="VFQ61_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954651.1"
                     /translation="MSVDVVAGGGALLVLSKGERSNGRPHALLRIMSESYELNRVSWA
                     WVGTKWLILALPLLNVGCSGQADFEPRAVGGAAGEFGEFRGGAGGLAVGGATGGFGEV
                     PQLKVIGATRRDEHQLDVEVSTSAPLYYWDCLDDPRLLKSDGTPFRNEVPACGVAPYY
                     LDGTYVENPWMFRCIGCDFSPCLPFPKSIWFSTDELVLAENEGTELPTDVQHFETLTT
                     RGPYLLRAKYFTSPTCQGEALSGAVTVDIQ"
     gene            complement(5662..6489)
                     /locus_tag="VFQ61_09110"
     CDS             complement(5662..6489)
                     /locus_tag="VFQ61_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954652.1"
                     /translation="MLACMGLSFACSGQAQHQATALGGTSGEAATTQGGSGGRNPENT
                     GGTARDDRSGGKSGSAGSTVLGGVETGGGGDTGGRAPGAAGETIYNETGGSGGTTGHE
                     SPIRIVGVKRQGDSSIVVEFATATPIYQRTCTGQMALTKSDGKAFTNELPACGSIPYY
                     LDGAFNANEWYLGCLGCDVQYCNPFPKKWSTGTNEIVRVQVAATGGEGGVSGAAGGAG
                     SSGEAGGAAGQADVPVDALHFETRTTEGPYWLTARYFTSPTCDGPQLTTELLLIDVR"
     gene            6676..7089
                     /locus_tag="VFQ61_09115"
     CDS             6676..7089
                     /locus_tag="VFQ61_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9954653.1"
                     /translation="MPRILINLDVDDVERAIRFYTEAFDLRLGRRFDAEFVELIGAEA
                     PIYLLAKANGSQPFKSASVGRDYGRHWTPMHLDFVVENLDAALARAQAAGARVESEIS
                     EHAYGRLVLCSDPFGHGFCLLEFRGRGYGELVRTG"
     gene            complement(7104..7844)
                     /locus_tag="VFQ61_09120"
     CDS             complement(7104..7844)
                     /locus_tag="VFQ61_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954654.1"
                     /translation="MSRSTSKSGLGSSWLILGLAILGTACSGDAKRDPHGAGGSTAGQ
                     AGAAQGGTGGSITGGVGGYAGEEPSFSVVGANRPDPHRIVVEFATSGPIYQWTCSGAL
                     ALTKSDGSAFRRELPECGTTVPYYLDSTYYPNEWHLSCLGCDVVSCFPFPESWTVSTD
                     EIVRAEAAPIPILGEGGVSGAGGSTGEGGSASETAGAAGSADVPINAQYFQTRTTQGP
                     YWIRAKYFTSPSCSGTELTSDPVLVDVR"
     gene            8183..9094
                     /locus_tag="VFQ61_09125"
     CDS             8183..9094
                     /locus_tag="VFQ61_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET9954655.1"
                     /translation="MLDLNDFYFFVQVVDGGGFAATGRTLRVPKSTLSHRVQQLESEL
                     GVRLLNRSSRKFGMTDAGTDFYRHATAVLREAELAETAVRQRLTEPTGIVRCTASSAT
                     MQFAMSKIIAEFLVQYPKVSIVAHSSNRKVDLVGENYHLAVRAHSLPLPDSNLVQRTL
                     ADAPWFLFAGTEYLRTVGAPQTPEDLQKHPSMLTLRAGKEPVWRLRHAQQKRDEVVVP
                     LTPRLLSDDLVGLQQAAIRGLGIVALPGYICRAAVQSGALQRVLPSWVAGDSKLTALM
                     PHKQGLLPSVRAFLNHLAVELPKKVEL"
     gene            9367..10305
                     /locus_tag="VFQ61_09130"
     CDS             9367..10305
                     /locus_tag="VFQ61_09130"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9954656.1"
                     /translation="MFDSKPHSDPQTTIVHISPAERERERSLRERFAQFWGTATGEIR
                     DVYDRFVAEVPLTEDVSLEPVNDDRVRGWWVRPPHAEASRAILYLHGGAYVLGSAQAY
                     RGFVSQIVSRTRVPALVLDYPLAPEAPLPAAPLAAQEAYTLLTQQGFERVALVGDSAG
                     GGLALVTLAELVKQRRGAMPIAGVVFSPWTDLALTGASLTDPSVVDPLLSYELLQDSS
                     RKYVNGHEPTNPLASPLYADLRRLPPLLIQVGTDERLLDDSRQFTERARRAGVSAELE
                     IWEGMHHVFQRDVEHLESSRNALDRAARFLRKTLKS"
     gene            complement(10349..10930)
                     /locus_tag="VFQ61_09135"
     CDS             complement(10349..10930)
                     /locus_tag="VFQ61_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9954657.1"
                     /translation="MHPARARFTFEEYLRVEEQSSIKHEFYEGEVYAMAGGTPEHAAL
                     SARLAVALGAQARQGGCEVFTSDLRVRIAATGLVTYPDLTLICGAWQRDPSDSRTVLN
                     PTLLVEVLSASTASDDRGEKLANYQRIETLREVLLVAHDSPRLELWRKDETGRWLLLV
                     ANAGDALRLASVDTSLDVDDLYAGLLPSSSAAL"
     gene            complement(11039..>12465)
                     /locus_tag="VFQ61_09140"
     CDS             complement(11039..>12465)
                     /locus_tag="VFQ61_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954658.1"
                     /translation="LDVQDVTTALLIESDPATRRACERAQKQPSLQPEMRFLTRAICH
                     DRLKRAPLPEEPPAIDRSSMGALVTTVAAHRARGGREETRKRVELLRAVEKDAKTSVE
                     RAILLVTMAELLYNAGDLQGAQSSARMAGRLLPKLVDLRGTPWHRLTYSSEFDRAISR
                     VHSAWVPWEPIAATNTGSRDPDLNERARMAGRAQLLCRRGYFAAAYGEVLAVQGKTEE
                     ARNVAEQLDDDYLRVRALSGATQYRRGLELATRVLSNMPAEDVNAGKAFRVAAAGAEA
                     ARYLGHPAAFVDDLVRRFIEAEPPHVHVGVSPFFSLVYACMEAPKPVARRCLKRLRFV
                     YQRGDFGGVIGVVPMILEGADQWIAGDAHGAARAWRPMLRQGGNIGEAAFRHILTEAL
                     EKAEMPELAARIDARILQLVDHPDAVDLAFARAAVRAEKSGDIAQARKLAQACVERWQ
                     HADDDIPARREMQALLERLNKKQH"
BASE COUNT         2249 a         4024 c         3995 g         2197 t
ORIGIN      
        1 gccgtccccg aagccgacca tcgctttgac ctcgggcaag aggacctcga gcacgcccga
       61 caccaacagc gcgtccaaac ctaggtccgc atgctgaccg agcatcaccc gatccagcgc
      121 ccgacgcacg tccgggcgcg agaggcacgc caaatcctcc gcggagatca gagtgacggg
      181 gcgatcggag aaaccgcgct ctgacgccgc cacggcccgt tccaaggcgt gcatggcgtg
      241 ctcttttcgt ccgctgagct ctatttttcc gctaatcggg ggggtggtcg acatgctgga
      301 gggctccccg ggcgggaagg gggtcggcac ttctacgttg gcctgtccaa agcgcaagcg
      361 ggaaaacgcc ggtcatcccc tcccggggcg agccctccgc caactgcccg agaatggcca
      421 agaaagctgc gcgaatgccc gaacccgggg ccgcggggtg acgagccacg ggcgattcgc
      481 gatttgagag atacgcttcg ggcagtgctt ctccgccccg ttctccgacc cggccgccgt
      541 gtggatctgg gacgctggtt cgcgcgcttc gcgtgcgtgg tgttcggact acttggtgcg
      601 ctgccgttgc tcgtggaggg ggtgttgcga ctgcactcgg tgcaaagcta cgtcgaccga
      661 cagacggcgc agctgctcag tcgtgagctc ggcctagtgg cgagttacga agtgaccgca
      721 cacctgatcc cactgcggct gacggtggag cgactgcggg ttccggcgag tgacggcggc
      781 tcgcccgcgc tcgaggtgga gaaggtgatc gtcacgccgc gcatcttcag cttgctcgcg
      841 gggcggctcg acgtcggcaa cgtggagatt gaatctccgc gcgcgcgttt ggtgctgcgc
      901 gacggcaagt tgaccaacgt caagtacacg ctgaagccga gcaagggcag ccccaagccg
      961 agcaaggaag cgccgttctc cgcgctatcg gtgagcgagg cgcgcgtgtc gctcgatctc
     1021 gacggcctgc aaatcgagag tggccccacc gacatcgatg tcttcgcgga gagtggcccc
     1081 gccttcgaag tgatgttgag cggcgccgcc acgaacctgg agtggcgcca cccacccaag
     1141 cgcggcacct taccgatcga agtcgacgaa gaccgcctgt gccgactgga tttgcgcgcg
     1201 cgcatcgaag gccgccaatt cttggtgcgg cgcttgtctg cgctcggtgt gagcgacttg
     1261 gatcccgcgc cgggcagcac gccttcgtgc gacgtcggag atggtgcggt gggacgggtc
     1321 gcgctccggc tctcgcagct ccgtgtgtct ttgccagagc ggggcgcgcc gttggtttcg
     1381 gggcatgtgg tggtgcgcgg gccgctcgcg ctgacgaatc ggttcgtggc gactcaaccg
     1441 ttggcgggtt gggccgggtt cgcgggtgac gtgcgttatg acgggcgcac tcgcttaccg
     1501 ctgatccagg gtcacgtaag cggcgcgggc atcgctttcg aacgttatcg cttggcgagc
     1561 tcgctgtccg tggacgtgga cgtgcggggc gacgtggtgc gggtgcctcg ttacgagatg
     1621 gacttcgcgg acgggcacgt cgtgctcgag aatgcgcgga tcgatccgtt cgccgaaggc
     1681 gtgccgctat ccgtgtcgcg cgtggacagc cgggccctca ccttccctgg tttgatgcgc
     1741 gacttggggg tgacgccgaa cacgatcgtg gcgtggaaca tgatcgacac gctggtctcg
     1801 aacttgaaag gcacgctctc gccgctcaag ttcgacgccg atttggcggc cgacaccaag
     1861 gacttcgagg tcaccgaccg cgccttccat gacccccacc gcaagcacat ggtgggggta
     1921 aagagcgccg aaattcgcgg caagatcggg gtgcgcccga acgcgctgat tttctatgac
     1981 acgcgcgcgg agttcgggaa gagctcgatg caagtgaagc tggtctcgat tggcttcgac
     2041 aatcgattgg agctcgagat cggtaagggc tcgcggttgc acttggcgga cgtgagcccg
     2101 atcgtcgata tcccggtggc gggcgagtcc gagctcgacg cgagcatgtc cggggccgcc
     2161 aacgatccgg tgctgctcgg caacttgagc gtcaaaggct tcgagttcgg cggcttcccg
     2221 ctcggagata tcaagagcgc caaagccaag tttcgcccgt tgtttctcga gctctcggac
     2281 gtgctctcga ccaagggcag cagtgacttc acggtgccgt tcgcgcgcat tgacttcggc
     2341 acgcgcgcgt ccgtggcggt gcaagcccaa gcgcgaagtc ccaagctgga cctgcgggac
     2401 ttcttcaaga tgtggcactt cgacgaggat ccgcgcttcg agccgattcg aggcacgagc
     2461 gcggtggacg cctcgattcg ctacgtgctc ggcgggcccg aagatcgctg cggcggcgga
     2521 gtgctgcgcg tgcaggggca actcaagacg cgcgagctcg agctgtacga agagcactac
     2581 gacgggggcg agagcagctt cgatttcctc tggcacgacc gcgaggccgc atatcgaggc
     2641 gtgatcttca acgcgcctga cttcacgctc aataagggca gcggacgcat cgcgggcgag
     2701 ctgacgatgc aaaccggcgc caaggtcgcc ggcaaagtgg tcgcgaccgg gatcccagta
     2761 ggcaagattg atgcgctccc caccctgctc cgcgccgctg agggcacggt caacgcgacc
     2821 ggcaccgtgt ccgggacgct cgatgcgctg gtggccgaca tcaacgccaa cgtctccgcc
     2881 attcagctcg ggctcggcag cttgcccgct tcgaccttcc gagtgcatct gcaaccaacg
     2941 atccagccgg agacggtgat cggaaagacg cgctgcggag agcctatctc gggccccttc
     3001 gaacgggcgc aatacgatcg cgacctagag agcggcgtgt tctacacgga cggcgagctc
     3061 ttcggcgggc agatcgtact cgagcggctc tcggtcacgc gccaacgtga caagacggtg
     3121 cgcggcaagg tgcatttcag agatctcgga ctcggcgcgt tgctccagct cggccgcagt
     3181 acggcgacac cgagcacacc catgtcgggg ctcgcgtcgg gcacgctgag cttggatgag
     3241 ctgcgcatgg ccgatcccaa ggccgcgcgc ggcacgttgg cgctcaagac attggccgtg
     3301 gagcgacagg gttaccgcgt gcagctgttg gacggcgcgc aacccttccg gattggcggg
     3361 cgcgccgtgg acctgccgag cgccgcgtta caagtcgaaa cccctgaagg tcaaaaggcg
     3421 gtcttcgacc tgtccggcag ggtctccaac ttggggcaga atccgcaact cgacgccggg
     3481 gttcagatgc gacccacggc gctggcgagc ttggtggggc tcgtgccgcg ggtggagcgg
     3541 gccaatggcg tcttgtctgg gcgtttccgt ctgaccggtg cgctcaatgc tccaagcacg
     3601 acgggcaccc tcgatttgca gcgtggagag ctagctttgc gcggtctacc gacgccgctc
     3661 agcgacatcg acgttggcgt tgccttcgac cacggcgagc tgcgtgtgga acgcggctcc
     3721 gcgcggctcg gcagcgggag cgtgagcttc agcggctcgg cgccactgcg tggtttctcg
     3781 attggcgaag gccgcttccg cgccgtcgcc cacgaggtct cgctgccact caaggagggc
     3841 atccacacga atctcgacgc cgatttgcag atcagcatcc gccccgcggg agacagtgaa
     3901 actcgcccgc tgccgcgtgt gaccggcaac gtgatgctgc gctccttcga atacaagcgc
     3961 cccatcacca tcgccgccga tctgaatacg ctcgcgcaac gcggcaagcg caccgccatc
     4021 gaagattacg actcacgcga cgacgcggtg gagttcgacg tcaacttgcg ctcggggcgc
     4081 ccgctcaaga ttcaaaacga catcgtcgaa gccgagctcg tgctgtccga agatggcctc
     4141 gagctcgccg gcaccaatgg gcggttcggt ttgcgcggca acgtgcggct caaacaaggc
     4201 gggcgcgtca ccctgcggcg cagcgaattc gagatccaag agggctccgt ccgcttcgac
     4261 gacctcacgc gcatcgcccc actcgtcgac gtgagcgccg tcaccgaata tcggcgctac
     4321 tccgacgccc ctggcaccac cagccaaagc accaccaaca ccgccacact gaccggcacc
     4381 tccaccaccg tcaccggtgg gcgctggacc atccgcatgc atgcctacgg cgacgcggac
     4441 aatctcaaag tcgatctgag cagcaccccg gccctggccc aagacgacat cttcttgttg
     4501 ctcacggtcg ggctcacgcg cgccgagctc gatcaagccc agagcgcgag cgtgggtggc
     4561 agcgtcgcgc tcgaggcgct cggcaccctc accggtgcgg accgggccgt aaagaaggtg
     4621 gtgcccatca tcgacgagtt ccgcttcggc tccgcttatt catcacgcac gggccgcacc
     4681 gaacccaccg tcaccgtcgg caaacgactc accgagcgcg tccgcgccaa catcaccagc
     4741 ggcctctccg aatcgcgtga agtccgctcc aacgtcgagt ggcgcctgtc ccccaaggtc
     4801 agcgtcgaag gctcctacga caacgtcaat gacatctcga gctcggcgct cggcaacctg
     4861 ggcgcggacg tgcggtggcg gctggagttc gagtagctcg ccctgaaatc aatccggcgc
     4921 gctgctcatt gtatatccac tgtgaccgcc ccgctcaggg cttcgccttg gcacgtgggc
     4981 gaagtgaagt acttagcccg gagcaggtag gggcctcggg tggtgagagt ttcgaagtgc
     5041 tgaacgtcgg tcggaagctc cgtaccctcg ttctccgcaa gcaccaactc gtccgtagag
     5101 aaccaaatcg atttggggaa gggcagacag ggagagaagt cgcatccgat acagcggaac
     5161 atccaaggat tctcgacgta ggtcccgtcc aggtaatagg gcgcaacacc acaggccggc
     5221 acttcgttgc gaaatggagt accgtccgat ttgagcaacc ggggatcatc taagcaatcc
     5281 caatagtaga gcggggccga ggtcgagact tcgacgtcga gttggtgttc atcccgacga
     5341 gtcgcgccga tgactttcag ctggggcacc tcgccaaagc caccagtcgc gccacccacc
     5401 gcgagccctc cggctcctcc gcgaaattct ccgaattcgc cggctgcgcc gccaaccgct
     5461 cttggctcga agtcggcttg cccgctgcaa cccacattga gcaatgggag cgccagaatg
     5521 agccacttcg tgccaaccca cgcccaggaa actcgattca attcgtacga ctccgacatg
     5581 atgcggagaa gggcatgcgg cctgccattt gaacgctcgc ccttggagag caccagcaga
     5641 gcccctccgc cggccacgac gtcaacggac atcgatcagc agcagctcag tggtcaattg
     5701 agggccgtcg caggtaggcg aagtaaagta cctcgccgta agccagtacg gtccttccgt
     5761 cgtacgggtc tcgaagtgca gagcgtcgac gggaacgtcg gcctgccccg ctgctccgcc
     5821 ggcctcgccc gaagaacccg cgcctcccgc agccccgctc acgccgcctt cgcctcccgt
     5881 tgcagcgacc tggactctca cgatctcgtt ggttcctgtc gaccactttt tcgggaatgg
     5941 attgcagtac tgcacgtcgc aacccaagca gcccaggtac cactcgttcg cattgaacgc
     6001 gccatctaga tagtaaggaa tcgatccaca ggcggggagc tcgttcgtga aggcctttcc
     6061 gtcggacttc gtgagtgcca tctgtcccgt gcaggttcgt tggtagatcg gagtcgcggt
     6121 cgcgaactcc acgacaatgg agctgtcgcc ttgacgcttc acgcctacga tgcggatggg
     6181 agactcgtgg ccagtggtgc cacccgagcc acccgtctcg ttgtagattg tctcccccgc
     6241 tgcgcccggc gcgcgaccgc ccgtgtctcc gcctcctcct gtttcgactc cccccagcac
     6301 cgtgctgccg gcgcttcctg acttcccgcc actgcgatcg tcccttgcgg tgccgccggt
     6361 gttttcgggg tttcgcccac cgctaccgcc ctgagtcgtt gctgcctcac ccgaggtgcc
     6421 gccgagcgcc gttgcctggt gttgagcctg tccgctgcac gcaaaactca gtcccataca
     6481 ggccaacatg agccaacctg tgccggcagc agaattaagg ccgcgattcg ggagcatcga
     6541 agtccgcaaa acaccagaga tagttcggaa catgaggtgc gcgacagcac gcagcgtgcc
     6601 atcgcggggc tccagggacg gccggagtcg acctgcggcg ggccatcgtg caaagctgga
     6661 gttggaggac gccggatgcc ccgcatattg atcaatctcg atgtcgatga cgtcgagcgc
     6721 gcgattcgct tttacaccga agccttcgat ctgcggctcg gacgccggtt cgacgccgag
     6781 ttcgtagagt tgatcggcgc cgaggcgccg atctacctat tagcgaaagc caacggcagt
     6841 cagcccttca agagtgcgag tgtgggccgc gactacggtc gtcactggac cccgatgcat
     6901 ttggacttcg tggtcgagaa cttggatgcc gctctcgccc gcgcgcaagc ggcgggcgct
     6961 cgggtggaaa gcgaaattag cgagcatgct tacgggcgct tggtgctctg cagcgatccg
     7021 ttcgggcacg gcttctgtct gctcgagttc cgcgggcgcg gctacggtga gctcgttcga
     7081 acgggctgac cgcaatcggg tgatcaacgc acatcgacca gcacggggtc gctcgtcagc
     7141 tccgtgcctg agcaacttgg tgaggtgaag tacttggctc gaatccagta ggggccttgg
     7201 gtcgtgcgag tctggaagta ctgcgcattg atgggaacgt cggcgctgcc agcagcgccc
     7261 gcggtctcgc tggcagagcc gccttcaccc gttgaaccgc ccgctccgct gacgccgcct
     7321 tcgccgagta tggggatggg cgcggcttcg gcgcgcacga tttcgtcggt ggagaccgtc
     7381 caggactcag ggaatgggaa acacgatacg acatcacaac ccagacaaga gaggtgccac
     7441 tcgttggggt aataagtgct gtcgagatag taggggacgg tggttccgca ctccgggagt
     7501 tcacgtcgaa acgccgagcc atcggacttg gtgagcgcga gcgcgcccga gcaggtccat
     7561 tgatagattg gtcccgaggt cgcgaactcg actacgattc ggtgcggatc cggacgattc
     7621 gcgccaacga cgctgaacga gggctcttca cctgcgtagc ccccaacgcc tcccgtgata
     7681 gagccacccg tcccgccctg agccgcgccg gcttggcccg cagtgctgcc cccagcgcca
     7741 tgcggatcgc gtttggcgtc gccgctgcat gccgtcccga gaatggccaa accaagaatg
     7801 agccagcttg agccgagacc ggacttcgaa gtcgaacgag acatgtggta ccgggaaaga
     7861 cgcacgctct gacgagacat gaacggtccg agtagcactc attgtgccat ccagacgagc
     7921 cgagttcgga gctgacgcaa gctcggcact tcaagctcgg tactgtcaac ctcggtctgc
     7981 ccacgctcgt gtgagaacgg ctcagctgta tactcgagag gcgggtctgg actagtgtgc
     8041 gcgggtgtca gctgcggtct ggatccgtcg cgttgccaag tactcactct tcgcggttgc
     8101 gggagcgctg ctgctcgcgg cggcggtggc actgaaacag tcgtcccgag agcgccactc
     8161 caattgaggg tcgaaggcga gcatgttgga cctcaacgac ttctatttct tcgtgcaggt
     8221 ggtggatggg ggaggatttg ccgcgaccgg gcgcacctta cgtgtgccaa agtcgacgtt
     8281 gagtcaccgc gtccaacagc tcgaaagtga gctaggggtg cgcttactga accgtagctc
     8341 gcgtaaattc ggcatgaccg acgcagggac cgatttctat cggcatgcga cggccgtgct
     8401 ccgcgaagcg gagctcgctg agacagcggt tcgtcagcgt ttgaccgaac ccacgggtat
     8461 cgtgagatgc accgcgagct cggccaccat gcagttcgcg atgtcgaaga tcattgccga
     8521 attcctcgtg cagtacccga aggtgagcat cgtggctcac tcgtccaatc ggaaggtgga
     8581 cttggtcggc gagaactacc acctcgcggt gcgagcgcat tccttgccgc tgccggattc
     8641 gaacctggtc cagcgcacgc tggccgacgc accttggttt ctgttcgcgg gcactgaata
     8701 cctgagaacc gtcggagccc ctcagacgcc cgaagatctc cagaagcacc cctcgatgct
     8761 gacgcttaga gcaggcaaag aacctgtctg gcggctccgg cacgcccagc aaaagcggga
     8821 cgaggtggtg gtgccattga caccaaggct gctgagcgac gatctggtgg gccttcagca
     8881 ggccgcaatt cgaggcctcg gcatcgtggc tttgccgggc tacatctgcc gcgctgcagt
     8941 ccaatcgggc gctctccagc gggtgcttcc gagctgggtt gcgggggact cgaagctcac
     9001 cgcactcatg ccccataaac aagggctctt accgtccgtg cgggcgtttc tgaatcatct
     9061 cgcggtagag cttccgaaga aggtggagct ctaagcctgg atttcgcgcc gacgacggac
     9121 gaagtcggca agaccgaagc gagatcggct tcagattggc tgacggcgcg cgcagccata
     9181 gcagggctat ggcgagcacg tcggcaccta agatgaggcc gagaccgcga ggaattgccg
     9241 gcttcggact aggaggcgcg aaatccaggc taagcctgag accgagcatc agcgtccaag
     9301 gcattggacg ctaagtctct cacggtacgt cttttggatc gcggccgcgg ggagaagatc
     9361 tgctccatgt ttgactcgaa gccgcactcc gatccccaaa cgacgatcgt ccatatttcc
     9421 cccgcagagc gcgaacgcga gcgctcgctc cgcgagcgtt tcgcacaatt ttggggcacg
     9481 gcgaccggcg agatccggga cgtctacgac cgcttcgtcg ccgaagttcc gctgaccgaa
     9541 gacgtgtcgc tcgagccagt caatgacgac cgagtacgag gctggtgggt gcgcccgccg
     9601 cacgccgaag ccagccgagc cattctctat ctacacggcg gcgcttacgt cctcggctcg
     9661 gcccaggcgt atcgcgggtt cgtgagtcag atcgtcagcc gtactcgcgt accggcgctg
     9721 gtgctcgact atccgttggc tccggaagcg ccgttgccgg cggcgcctct cgccgcgcag
     9781 gaggcgtaca cgctgctgac ccaacaaggc ttcgaacgag ttgctctcgt cggagactcg
     9841 gcgggcggtg gactcgcgct cgtgacgctc gctgagctcg tgaagcagcg gcgcggagca
     9901 atgccgatcg cgggggtcgt gttctcgcct tggacggacc tggctttgac aggcgcctcg
     9961 ctcacggacc cgagcgtggt ggatcccttg ctcagctacg agctcctcca agacagttca
    10021 cgcaagtatg tcaacggcca cgagcccacg aacccgctcg cttccccgct ctacgctgat
    10081 cttcgcaggc tgcctccgct gctaatccaa gtcggtacgg atgagcgcct actcgatgat
    10141 tctcggcagt tcaccgagcg cgcacgccgc gccggcgtct cggcggagct cgaaatttgg
    10201 gaaggcatgc accacgtgtt tcaacgcgac gtcgagcacc tggaaagcag ccgaaacgcg
    10261 ctcgatcgcg ccgcacgatt cctacgcaaa acgctgaaaa gctgaagaga gactcattcc
    10321 ggtgcactcg cgcactcgcg agtgcacctc aaagcgcggc ggagctcgac ggcaacaacc
    10381 cggcatacaa gtcatccacg tccaggctcg tatcgacgct ggcgagacgc agtgcgtctc
    10441 ctgcgtttgc cacgagcagc agccaacggc cggtctcgtc tttacgccag agctccagtc
    10501 gagggctgtc gtgggccacg agcaatacct cacgcagggt ctcgatgcgc tgatagttgg
    10561 cgagtttctc gccgcgatcg tcacttgcgg tggaggcgct gagcacctcg accaagagcg
    10621 tcggattgag tacggtgcgt gaatccgacg ggtcgcgctg ccatgctccg cagatcaacg
    10681 ttaggtcggg ataggtgacc agacccgttg ccgcaattcg tacccggaga tccgaagtga
    10741 agacttcgca tccgccctgc cgcgcctgcg cacccaatgc cacggccaag cgcgccgaga
    10801 gcgcggcgtg ttcaggcgtg ccccccgcca tggcgtaaac ctcaccctcg tagaactcat
    10861 gcttaatcga gctctgttct tcaacgcgta ggtactcttc aaaggtgaag cgagctcgcg
    10921 ccgggtgcat cgtgacggtg taccacgcaa gtcaaagctg tcgaaacggc aggggctgca
    10981 ccaccttcgt ctcgcgcgaa gctcacggcg aaaggggtta tcccctgagc cgtcaggcct
    11041 aatgctgctt cttgttcaat cgctccagca gtgcctgcat ctcacggcga gcgggaatat
    11101 cgtcgtcggc gtgttgccag cgttcgacgc aggcttgcgc gagcttgcgg gcctgcgcga
    11161 tgtctccgct cttttcggcg cgcacggcgg cgcgcgcgaa ggcgaggtcg acggcgtcgg
    11221 ggtgatcgac gagttggagg atgcgggcgt cgatgcgcgc cgcaagttcg ggcatttccg
    11281 ccttttcgag cgcctcagtg agaatgtgac ggaacgccgc ttcgccgatg ttgccgcctt
    11341 gccgcagcat gggccgccag gcgcgcgccg cgccgtgcgc atcacccgcg atccattgat
    11401 cggcgccctc caagatcatg ggcacgacgc caatcacgcc gccaaaatcg ccgcgttgat
    11461 agacgaagcg taaccgcttc agacaacgcc gcgcgacggg tttgggcgcc tccatgcaag
    11521 cgtacacgag tgaaaagaag ggggacactc cgacgtgcac gtgcggaggc tccgcctcga
    11581 tgaaacgccg caccagatca tccacgaacg cggccggatg ccccaagtac cgcgcggcct
    11641 cggctcccgc cgcggcgacg cgaaaggcct tgcctgcatt cacgtcttcg gccggcatgt
    11701 tggaaaggac gcgcgtcgcg agctcgagcc ctcggcggta ctgagtggcc cccgacaaag
    11761 cgcgcacgcg caggtaatcg tcgtcgagct gttcagcgac gtttctcgcc tcttcggtct
    11821 tcccctgcac cgcgagcacc tcgccatagg ccgccgcgaa gtagccgcgt cggcagagca
    11881 gctgcgcgcg ccccgccatc cgagcccgct cgttcaagtc cggatcacgc gagccggtgt
    11941 tggtagccgc gatcggctcc caagggaccc acgcggagtg aacccgcgag atggctcgat
    12001 cgaactccga ggagtaagtg agccgatgcc agggcgtacc gcgcaaatcc accaacttcg
    12061 ggagcaagcg ccccgccatt ctggccgagg actgcgcgcc ctgcagatct cccgcgttgt
    12121 agaggagttc ggccatcgta acgagcaaga ttgcccgctc gaccgaggtc tttgcatcct
    12181 tctcgacggc ccgcagcagc tcgacgcgct tccgagtctc ctctcgcccg ccacgcgctc
    12241 gatgggccgc cacggtcgtc accaaggcgc ccatcgaaga gcggtcgatt gccggcggct
    12301 cttcgggcag cggggcgcgc ttgagccggt cgtggcaaat cgcgcgtgtc aagaagcgca
    12361 tctcgggctg cagactcggt tgcttttgcg cgcgctcaca ggcccgacga gtcgcgggat
    12421 cggattcgat gagcaacgcg gtggtgacgt cttgcacgtc gagcg
//
LOCUS       DASTKU010000250         4152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1164786, whole genome shotgun sequence.
ACCESSION   DASTKU010000250 DASTKU010000000
VERSION     DASTKU010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164786"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1041
                     /locus_tag="VFQ61_09145"
     CDS             151..1041
                     /locus_tag="VFQ61_09145"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET9954659.1"
                     /translation="MVLANPSWVEKIAFGIDHVESVFVEDDGTLFAGGEKGQIYRVAP
                     GEPPAQIACTHGELLGIVLDGGGRIYVCDSANRAVFRISPAHDLQHEDRQHIAGAAQN
                     APVECLSTGAPGRPFTFPNHPAFDALGNLYVSDSGDYWDPSGSGCIQVIRRSGETEVF
                     HHGPFRFANGIAIDPSGKWLYIAQSAAWNIVRIPLDRPNGQVELAFQLPEYTIPDGML
                     FSSDGSLIVACYRPDSVLRCKPDGGVETLIADPTGELLLAPTNVFLHAGYLYVANLAG
                     RFVSRMKTELRLGSVHRPVI"
     gene            1057..2427
                     /locus_tag="VFQ61_09150"
     CDS             1057..2427
                     /locus_tag="VFQ61_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9954660.1"
                     /translation="MTVQQSVRSIERVHMPRVLGQETELRTSRGKLVRIVQLNNAATA
                     PPFESTVQRLHQFLETYGALHRGAGPRARLTCEYVEEAKARIRAFLGCPEGHHVLFTE
                     NTSAAVNLLARLLNLGEKDVVLTSELEHTSKYLPWRFNTRAEVVEVAARDDGSLMMGD
                     FEEKVLRYRDRLRVIAISGASNLTGYVTDLPALAKLAASSGALLFVDAAQLAPHRPID
                     MARDGIGALAFSAHKLYAPFGLGVLVVPRKILERAPVSPGGGSIDMISRERVMWSPAD
                     ERHQAGTWNASGIVALGTSCATIQEAGWSGILEHERMLVDYAARRLSRVPGLTLHVAA
                     EKFGEQGRIGTFPFTLDGYQPFHLAAILEHEHGIEVRAGTICNHRLVRRWFNVDEAGQ
                     AAVESAIEQGNRLASYGIARASLGIHNTTEDVDRLVDALSAIRMHGPALKYRPVPEHE
                     SYELVE"
     gene            2435..>4152
                     /locus_tag="VFQ61_09155"
     CDS             2435..>4152
                     /locus_tag="VFQ61_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9954661.1"
                     /translation="MPVFTRQDLVSESARQYPDRAALAFRGATLTYAELCSQMWGWAH
                     QLREVGVAKRDRVGVLMNRSFESYVAILGALQAGAAYVPFDVTAPRDRLVAVLRDCDI
                     RYLVVDRQNAPKLAEILADYPELRCIIAPEPVAGVRTVLASDIAPSSRPPDVRTVESD
                     LALIFYTSGSTGKPKGVAHSHRSMLSNVEWALQEFRFTHRDRFSNVTSHHFDLCWLEM
                     YASLACGGCIVIAPESSVRFPLELAALTAEQRVSVWCSVPSVLIGLLERGQLERHDWS
                     HLERVLFAGERFPVKHLHHLMGKLPNAQFCNMYGTTETHIAAFYPVLQAPAEDAAPLP
                     IGKPCRHVNLLVVGADGSPVEPGQTGQLVIRGPSQMEGYWGMPERTAQVVRGVREAAD
                     YAPDYYFTGDLVRVNEHGDIEVIGRADRRVKVRGYLVDLDEVEKVLLRHESVKEAGVF
                     VVGEEGVDARLEAAVVPKPGAEASPAELRYHAARTLPVYAVPESVYVVPEFPRTGSGK
                     LDRVSLRNMQPELSQEPASAGSLVEKTEADPRAEVKGYIANELALNSALVLDDQTDMI
                     ESGILDS"
BASE COUNT          755 a         1197 c         1373 g          827 t
ORIGIN      
        1 tgacagtccg gggtcaggtt cgtattaatg tcggcagtct tcaggtacgg agtgttggtt
       61 gtcgggtggt gttgcgaatt gggcgtaggt gttggccctg gggagtgtcg cccctcggga
      121 aaagattggg gtcagaagcg attggggtaa atggtgctcg cgaatccaag ttgggtagag
      181 aagatcgcct ttggcatcga ccatgtggag agtgtgttcg tcgaagatga cggaacactg
      241 tttgcgggcg gtgaaaaggg ccagatttac agagtagctc cgggagagcc gcccgcgcag
      301 attgcgtgca cgcacggcga attgctcggg atcgtgttgg acggcggtgg tcgcatctac
      361 gtttgtgatt ccgcgaatcg cgcggtattc cgtatttcac ccgcccacga tcttcaacat
      421 gaagatcgcc aacacattgc gggtgccgcg cagaatgcac cggtggagtg tctttcgacg
      481 ggggcgcctg gacgcccatt caccttcccc aaccacccgg cgttcgatgc cctcggcaat
      541 ctgtacgtct cggattccgg tgactattgg gatccgagcg gcagcggatg cattcaagtc
      601 atccgtagga gtggtgagac ggaagtgttc caccatgggc ccttccgctt tgccaatggg
      661 atcgcgatag atccgagcgg gaagtggctc tacatcgcgc aaagcgctgc gtggaatatc
      721 gtacgtattc cgctcgatcg gcccaatggg caggtcgagc tcgcgttcca gctccccgag
      781 tacacgattc cagacggcat gttgttcagc agtgacggtt cgctgatcgt tgcgtgctac
      841 cggcctgatt ccgtgctccg ctgcaaaccc gatggcggcg tcgagaccct gattgcggat
      901 ccgacggggg agctgttgct cgcaccgacc aacgtctttt tgcacgccgg ttatttatac
      961 gtggccaatt tggcgggacg tttcgtatcc aggatgaaga cagagctgcg cttgggcagc
     1021 gttcatcgac ccgtgatttg aattcggagg gatcccatga cagtgcagca atctgtgcgc
     1081 agcatcgaac gagtccacat gccgcgcgtg ttgggccagg aaacggagct caggacttcg
     1141 cgcggcaagt tggtccggat cgttcagttg aacaatgctg caaccgcgcc gccattcgaa
     1201 tcgacggtgc agcgattgca tcagttcctc gaaacatacg gcgcgttgca ccgcggggcc
     1261 ggcccgcgcg cgcgcttgac ttgtgagtac gtggaggagg ccaaagcacg gatccgcgcc
     1321 ttcttggggt gccccgaagg ccatcatgtc ctcttcacgg agaacaccag cgccgcggtg
     1381 aacctattag cgcggctgct gaatctcggg gaaaaggacg tcgtcctcac gtccgagctg
     1441 gagcacactt cgaagtatct gccctggcgt ttcaacacac gggccgaggt cgtcgaggtc
     1501 gccgcccgag acgatggctc tctgatgatg ggtgacttcg aagagaaggt gctccgctat
     1561 cgcgaccggc tgcgggtgat cgcaatcagc ggcgcgtcga acttgaccgg ctatgtcacc
     1621 gacttgccgg ctctggccaa gttggcagcg tctagcgggg cgctgttgtt cgtggacgcc
     1681 gctcaactcg ctccgcaccg tcccatagac atggcaaggg atgggatcgg tgcgctcgcg
     1741 ttctcggcgc acaagctcta tgcgccgttt ggccttggag ttttggtggt gccccgcaaa
     1801 attctggagc gagcacccgt gagccccggc ggtggatcga tcgatatgat cagtcgcgag
     1861 cgcgtgatgt ggtcgccggc agacgaacgc caccaggcgg ggacctggaa cgccagtgga
     1921 atcgtggcgc tcgggacttc gtgcgccacg atccaggagg cgggttggtc cggcatcctc
     1981 gagcacgagc ggatgttggt cgactacgcc gcacgtcgtc tgtcgcgagt gccgggcctg
     2041 acgctgcacg tcgcggcgga gaaattcggt gaacaaggcc gcatcggcac gttcccgttc
     2101 acgttggacg gttaccagcc gtttcacttg gcggccatcc tggagcacga acacggcatc
     2161 gaagtgagag ccgggacgat ctgcaaccat cgcctggtgc gccgctggtt caatgtggac
     2221 gaagcgggcc aggccgcggt cgaaagcgcg atcgagcagg gcaaccgcct ggcctcttac
     2281 gggatcgcgc gtgcgagcct cggtattcac aacacgaccg aagacgtgga ccgcctggtg
     2341 gatgcgcttt ccgcaatccg tatgcatggt ccggccttga agtaccgccc ggtgcccgag
     2401 cacgaaagct acgaactggt cgagtgaagg agcgatgccg gtgtttacac gtcaggattt
     2461 ggtgtccgaa tctgctcggc aatatcccga tcgtgccgcg ctcgccttcc gtggcgctac
     2521 gttgacctat gccgagctgt gctcccaaat gtggggttgg gcgcaccagc ttcgcgaggt
     2581 gggtgtcgcc aagcgagacc gcgtgggcgt cttgatgaat cggagcttcg agagctacgt
     2641 cgcgatcctc ggcgcgctgc aggccggggc ggcttacgtg ccgttcgatg tgaccgcgcc
     2701 gcgcgatcgt ctcgtggccg tgcttcggga ctgcgacatt cggtacttgg tcgtcgatcg
     2761 gcagaacgca cccaagctgg ccgagattct ggcggattac ccggaactgc gctgcatcat
     2821 tgcccccgag cccgtcgccg gggtgcgcac ggtgctggcg tctgatatcg ctccgagttc
     2881 acgcccgccg gacgtcagaa ccgtcgagtc ggacttggcg ttgatcttct acacctccgg
     2941 atcgacgggc aagcccaaag gcgtcgctca ctcgcatcgg agtatgctga gcaacgtcga
     3001 gtgggctttg caggagttcc gattcacgca tcgagatcgc ttcagcaacg tcacctcgca
     3061 ccacttcgat ctctgttggt tggagatgta cgcctccctc gcctgcggcg gctgcatcgt
     3121 gattgccccg gagagcagcg tgcgctttcc cctggagttg gcggccctga cggccgagca
     3181 gcgcgtgagc gtgtggtgtt cggtgccctc ggtcttgatt ggcttgctcg agcgggggca
     3241 actcgagcgc cacgattggt cgcatcttga acgggttttg ttcgccggag agcgcttccc
     3301 ggtgaagcac ctgcatcacc tcatgggcaa gctgcccaac gcgcagttct gcaacatgta
     3361 tggcaccacg gagacgcaca tcgctgcgtt ctatcccgtg ctccaggcgc cagccgaaga
     3421 tgcagctccg ttgcccatcg gcaaaccttg ccgtcacgtc aacctgctgg tcgtcggtgc
     3481 cgatggctcc cccgtcgaac ccggtcaaac cggacagctg gtgattcgcg gaccgagcca
     3541 aatggaaggt tactggggta tgccggagcg cacggcacaa gtggtgcgcg gagttcgcga
     3601 agccgcggac tatgctcccg actattactt cacgggtgac ctggtgcgcg tcaacgaaca
     3661 cggcgacatc gaggtgattg gccgagccga tcggcgcgtc aaagttcggg gctacttggt
     3721 ggacttggac gaggtcgaga aggtcctatt gcggcacgaa tcggtcaaag aagccggcgt
     3781 gttcgtggtg ggagaagagg gagtggacgc gcggctcgaa gctgcagtcg tcccgaagcc
     3841 gggagcggag gcgagcccgg cggagctgcg ttaccacgcg gcgcgtacgc tgccggtata
     3901 tgccgtcccc gagagcgtgt acgtggtacc cgaatttcct agaaccggta gcggaaagct
     3961 cgaccgggtg tcactgcgca acatgcagcc ggaactctcg caagagcccg cgtctgcggg
     4021 gagcctcgtg gaaaagacgg aagcggatcc gagggcagag gtgaaaggct atatcgcgaa
     4081 cgaacttgcg ctcaatagcg cgctggtgct cgatgaccaa acggacatga tcgagtcggg
     4141 catcttggac tc
//
LOCUS       DASTKU010000251         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_50308,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000251 DASTKU010000000
VERSION     DASTKU010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50308"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..359
                     /locus_tag="VFQ61_09160"
     CDS             168..359
                     /locus_tag="VFQ61_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954662.1"
                     /translation="MRKSGTGWNIAPLRSAKRVELVASGIEVSSKDGRTRRLGSSRAA
                     FAFSFGFASAALTHPSAGI"
     gene            1239..>2967
                     /locus_tag="VFQ61_09165"
     CDS             1239..>2967
                     /locus_tag="VFQ61_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954663.1"
                     /translation="MKAKTGMVCFVKDKRGSVRRRVACVSTTRGNGLLCAAMLALGSG
                     CSGDVSSMSGNDDTEEPVEEVIQHFDPSPSPEAKKGHVMITLKALQLLRDRGMLPPQL
                     DTPEAEALLVYGNNFGDNTGVGWPNVSERGNVPRTPVKNHMSQLIPRDGAAPFALTSD
                     SFKFDVELDWWDDPTVTTTASASLAWYPGAVDPLNDSEGNHVSDSARLIGSTIVDADL
                     ALDFIPSAVDCVASLFADPYCHSLEPKPQVKGYGFSLDNLYHYAYGDLRDFGVPYDDK
                     DTALRLYPLLPSHIPGYDEDESHRDLANKLTARMQNQVILEGADFGAQKYGAILYQLS
                     RRFFSNSRQPEPDLAQLIKVGNDVPGWRTGYMRGQGVIDDVYLEYPHTYLGGMPYTCA
                     APSEAWKQQAITAFANARAAVLELQAFGGGFGSQLFRARADAAVKAAAPYADVCAHGR
                     PTWPAWIKSGFKGADQPPQDDEDLLSLEVARPGRSDRAALIYLGWAVHMIQDSALPHH
                     VSGWTGNEHSAQDALGDLLFYYEDYAGRTVPQKTCVTQALPPGNPKIPPGGVKPPKCT
                     TTYVPHPYAQ"
BASE COUNT          579 a          917 c          940 g          531 t
ORIGIN      
        1 atcgcgccct cgacgtcatc gcccggcatc acttggacgg tagcggcggc ggcgttcgat
       61 agggcgccga gcaagaacga ccccgacagc agcacgctca ggatgacttt ggccatcccc
      121 gaagctacgg cgtttcgcat ccgcgccgca aggtgggggt gctgggagtg cggaaatccg
      181 ggacgggctg gaatatcgcc ccgctgcgta gcgccaagcg tgtcgagctc gtcgcgagcg
      241 gcatcgaagt gtcatcgaaa gacggccgca cgcgacgact cggctcgtcg cgtgcggcat
      301 tcgcgttctc gtttggtttt gcgtcggcgg cgctcacgca cccctcggct ggaatctgat
      361 gacttcacca ggatgtggtg gcggcctcgc aggagaacgt gccggtgggt ccgtcgtcgc
      421 ggagcaaagc ggtagttgtc ttcgagcaaa tcctcctcgg gactcgtggt cgtcgaagac
      481 tttcgaaaga tcatccagac gagctgactg atgcactgcg cctggccatc cagaccgcgt
      541 ctttgcgcat gatatcctgg gtcgtcttga tggtggtact catggcgatg ggttctgctg
      601 aagctaggaa cccagtgcgt cttcgaggtc tagaacggcc ttgaggtagt cgtctttacc
      661 gccgaacttt gtatgagtaa tgcgcatcat gtatccgagc cgcgaacacg ggagtgctcg
      721 agatggtgcc agcggtttcg ccgcacgagc gcgatcaact cgccggcttc gggacaattc
      781 cagagcttct gcgcgacggt cactgctcgg tgcaaagatc cgtccgcaaa cgttcacgta
      841 cgtccgcgga aaccaggagg aagttatgca acttgaccag cccctggaca gcgtcgccgt
      901 tacatttaca aggccacctg cggtgcccat accagccgga acgtgactga tggcaagcca
      961 atcaaagcag tcggatatgg gtttgccggg atgttcactg aaaggctcta aaaatggggc
     1021 tcccggggcc tctaagactc tggagcaagc gtgtgggaga gggcattgtg agttggctcg
     1081 ctgagctgct gagctcaact tgagccgcat gcgcagcgcc cgcaaagacg cactgattga
     1141 gtagccaatc tgggcaaccg ggcgcgcggc acatgcgttg caatgagcct ttccgaagag
     1201 caggcagcgg tgggcggcct gcaagatcgg agaaagcgat gaaagccaag acgggtatgg
     1261 tgtgttttgt taaagacaag cgagggtcgg tgcgtcgccg tgtggcgtgc gtctcgacga
     1321 ctcgcggtaa tggcctattg tgcgcagcca tgctcgcgct cggctcgggt tgtagcggag
     1381 acgtgtcgtc aatgtccggc aacgacgaca ccgaggagcc cgtcgaggag gtgattcagc
     1441 acttcgatcc atcgccgtca ccggaggcga agaaggggca cgtgatgatc accctgaagg
     1501 ctctccagct gctcagagac cgcggcatgc tgccgcctca gctggacacg ccggaagcgg
     1561 aggcgctgct cgtgtatggg aacaacttcg gcgacaacac gggggtcggt tggcccaacg
     1621 tcagcgagcg cggcaacgtg ccgagaacgc ccgtcaaaaa ccacatgagt caactgatac
     1681 cgagggacgg ggccgcgccg ttcgcgttga cctcggactc cttcaagttc gatgtggagc
     1741 tcgactggtg ggacgatccc accgtgacca cgacggcgag cgcgtccctc gcttggtatc
     1801 ccggagcggt cgatcccctg aacgattcgg aaggcaatca cgtcagcgac tccgcgcggc
     1861 tcatcggttc gaccatcgtc gatgcggact tggcgttgga ctttatcccg agtgctgtcg
     1921 actgcgtcgc aagcctgttc gccgacccgt actgccactc gctcgagccg aaaccgcagg
     1981 taaagggcta cggcttctcg ctggacaatc tgtaccacta cgcctacggg gacctgaggg
     2041 acttcggagt tccgtacgac gacaaggata cggccctccg cctctatccg ctcctgccct
     2101 cgcatatccc cggctacgac gaggacgagt cacaccgcga cttggcgaac aagctcaccg
     2161 cgcggatgca aaaccaggtg atcctcgagg gtgcagactt cggcgcgcaa aagtacggcg
     2221 cgatcctgta ccagctttcg cgccgcttct tttccaatag ccggcagccc gagcccgatc
     2281 tggcgcagct gatcaaagtg ggcaatgacg tcccgggctg gcgcaccggt tacatgcgcg
     2341 gacagggcgt catcgatgat gtgtatctcg agtacccgca cacgtacctc ggcggcatgc
     2401 cgtacacgtg tgccgccccg agcgaagcat ggaagcaaca ggcgatcacg gccttcgcaa
     2461 acgcgcgtgc cgcggtgctc gaactacaag cattcggcgg cggtttcggg agtcaactat
     2521 ttcgggcgcg cgccgacgcc gccgtgaaag ccgcagcgcc gtacgcggac gtctgcgcgc
     2581 acggcaggcc cacctggccg gcgtggatca agagcgggtt caagggcgcc gaccagccgc
     2641 cgcaagacga cgaggacctg ctctcgctcg aggtggcgcg cccgggccgt tcggatcgag
     2701 cggccctcat ctacctcggc tgggccgttc atatgatcca agactcggcg ctaccgcacc
     2761 acgtatccgg ttggaccggc aatgaacact cggcacagga cgcgctcggc gatctcctgt
     2821 tctattacga ggactacgcg ggacggaccg tcccccagaa gacgtgtgtc acacaggccc
     2881 tcccgccggg caacccgaag atccctccgg gcggcgtgaa gccccccaaa tgcacgacca
     2941 cttatgtccc gcacccttac gcgcaac
//
LOCUS       DASTKU010000252         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_271891, whole genome shotgun sequence.
ACCESSION   DASTKU010000252 DASTKU010000000
VERSION     DASTKU010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271891"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..>2115)
                     /locus_tag="VFQ61_09170"
     CDS             complement(121..>2115)
                     /locus_tag="VFQ61_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9954664.1"
                     /translation="EREVVDPLEEAFGAISGVERISSNAQDSLAILTVEFDFKKDLQE
                     ATQDIRDKISELRRDLPTEIEEPVLTRFDPNDLPIVSLTLSSKTKSEAELSRLADPWL
                     RGELRSVRGVADAKLVGAQERELTVALKPEALKATGVGVGSVVQALALQNLAAPVGRV
                     QTRWSERSIRLRGRLASPAEFEALVVASANGQVVRLGQVAEVRDGTEEARSSALFNEQ
                     PAVGIDLLKSKGYSTTTVSDAIRARVDAIGRKLPKDVQLSVVRDAGVRVRHSVEDVEL
                     SLVLGAALTVFVVFLFLNSWRSTVITGLALPVSVLASFIAVRAFGFTLNTMSLLGLSL
                     AIGILIDDAIVVRENIVRHMEMGKDHVRAAREGTAEIGLAVAATTFSIVVVFVPIAFM
                     GGIAQQWFAPFALTIAASVMVSLFVSFSLDPMLSSIWPDPAVESGKRGPIGRALIRFN
                     GAVEQLTDKYKKVISWALRHRLIMVALAVASFVLALGLPAAGILGSEFFPDQDRSEFR
                     IELRAPPGSSLDYTREKALQATRIARALSEVQYTYTNLGGQTGESVDEASIYVRLRPK
                     ADRARSQAEISRLLRQDVGRLGGVEASVSNSGFSPQKQIQIEVRGQDLAELTRLAERI
                     ADQVRKVPGAVDVGLSSKGQKPELEVELDRELAGGLVHRM"
BASE COUNT          403 a          646 c          658 g          408 t
ORIGIN      
        1 cgcggacgga cgcgcgatta acttctgcag ggtctttctg tccgcggcgc ttagcttcaa
       61 gtcgttgctt cgttccacgc atcgttcgac gaggcaaccc ctccaaaaat tgaactcaag
      121 tcacatgcgg tgcactagtc ctccggcgag ctcgcgatcg agctcgactt cgagttcggg
      181 cttttgtccc ttcgaggaca ggcccacatc gaccgcgccg ggcactttgc ggacctggtc
      241 tgcgatcctc tcggcgaggc gggtcagttc tgcgaggtcc tgcccgcgta cttcgatttg
      301 aatctgcttc tggggactga atccagagtt cgatacagag gcttcgacgc cgcccagtct
      361 ccccacatct tggcgcagca ggcgagagat ctcggcctgg ctgcgggcgc gatcggcttt
      421 gggtcgcagg cgtacgtaga tgctggcctc gtccacggac tcacccgttt ggccgccgag
      481 gttggtgtac gtgtattgaa cttccgaaag agcgcgcgcg atgcgcgtgg cttggagcgc
      541 cttctctcgt gtgtaatcga ggctcgaccc gggcggtgcg cgcagctcga ttcggaattc
      601 ggatcggtct tgatcgggga agaattcgct gcccagaatg cccgccgccg gtaagccgag
      661 cgcgagcacg aagctcgcga cggcaagcgc gaccatgatg agccgatgtc gcagcgccca
      721 cgagatgacc ttcttgtact tgtcggtgag ctgctcgacc gcgccgttga agcggatcag
      781 ggcgcgacca atcggacctc tcttgcctga ttccaccgca gggtccggcc agatcgagga
      841 cagcatggga tcgagcgaga acgacacgaa cagcgacacc atcacggagg cggcgatcgt
      901 cagagcgaac ggcgcaaacc actgttgcgc aatgccaccc atgaaggcga tcggtacgaa
      961 cactacgacg atggaaaacg tagtggcggc gacggctagg ccaatctccg cggtaccctc
     1021 gcgggcggcc cggacgtgat ctttgcccat ttccatatgg cgaacgatgt tctctcgcac
     1081 cacaatcgcg tcgtcgatca ggatgccaat cgcgagggat aggccgagca aactcatggt
     1141 gttcagggtg aagccgaagg cacgcaccgc gatgaaggaa gccagcacgg acacgggcaa
     1201 cgcgagcccc gtaatcaccg tggagcgcca tgaattcaga aacaagaaca ccacgaatac
     1261 ggtgagggcc gcgccgagca cgagtgagag ttccacgtct tcgaccgagt ggcgcacgcg
     1321 cacaccggcg tcccgcacga cactcagctg cacgtctttg ggtagcttgc gcccgatcgc
     1381 gtcaacgcga gcccgaatcg cgtcgctcac ggtggtagtg ctgtagccct tggacttgag
     1441 caagtcgatg cccaccgcgg gctgctcgtt gaacaacgcg ctcgatcgcg cttcctcggt
     1501 tccgtcacgg acttcggcaa cttgtcccag acggacgacc tgaccgttgg cgctcgcgac
     1561 caccaaggcc tcgaattctg cggggctcgc gaggcgaccg cgtaagcgaa tggagcgctc
     1621 gctccagcgg gtttgcacgc gccccacggg tgcagctagg ttttgaagcg ccaaagcttg
     1681 tacgaccgag cccaccccca cgccggttgc tttcagcgcc tccggtttga gcgcgaccgt
     1741 gagctctcgc tcttgcgcac cgacgagttt cgcgtccgct acgccgcgca cagagcgaag
     1801 ctcacctcgt aaccatggat cggcaagtcg actcagctct gcttcggact tggtcttgga
     1861 agagagggtc agtgaaacaa tgggcagatc gttgggatcg aagcgggtca ggacgggttc
     1921 ttcgatttcg gtaggtagat cgcggcgtag ctcggaaatc ttgtcgcgaa tatcctgagt
     1981 tgcctcttgg agatctttct tgaagtcgaa ttcgacggtg aggatggcga ggctgtcttg
     2041 ggcattcgag gagatccttt cgaccccgct gatcgctccg aatgcttcct cgagggggtc
     2101 gacgacctcg cgctc
//
LOCUS       DASTKU010000253         8727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_718639, whole genome shotgun sequence.
ACCESSION   DASTKU010000253 DASTKU010000000
VERSION     DASTKU010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718639"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFQ61_09175"
     CDS             complement(<1..319)
                     /locus_tag="VFQ61_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954665.1"
                     /translation="MPKTTITERGLAHEWFSGCEYVNPDVIAQEELGDWNDPQTVLRA
                     AQVAEERRETCLRERKSLAFETVFSAPDKPEFVRRAICAGYFVRIFFVGTQTPEINAA
                     RVAR"
     gene            1144..2730
                     /locus_tag="VFQ61_09180"
     CDS             1144..2730
                     /locus_tag="VFQ61_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954666.1"
                     /translation="MGSMNLANAGKYGSFLLFCVQLGCGRQLSPTQGWPVEAGATSTA
                     GSGGTGGGVAQHGGQTSGLGGSGPAWAPDARLVESGTRLKAVLYTGGGTEVFRHFHDE
                     KLGFDCEFVEAANGDVRCVPSTVAVLSYLDASCREPATFLDFGHLPKEGDWVRADAWG
                     SAGSCPFSLPDFAETYRIGERVYDEQVPGNERQLVYGMVDSQCQVVTSRHGKIDPPVH
                     RLIPVPREELVSARRQRWVASEQFNVVSLQAEDGASLTLGVETHDGAPCALQRDGRCV
                     PHVPAIMEDVRAPYSHALDSSCSQPAYAIPIAERCGTPEYAVRFSETGQIRVHELAPA
                     NTVYSLLPGRFGTEYCTAVVEAPGTYFGLGADVTETLPQAIPVRLGEGPLWTESFTAS
                     KGLPPLGTGRPLQQQTFIDATGAPCQPVLAMDSTLRCLRTQNAVLQSSFWADADCTLR
                     LSSLVLSADTNTRDLLEPEYTADGKLSGYHDLVPYSGPIYEASSGSCLRADPPEPHFY
                     LPNRRSGFETLPVIEEVRRE"
     gene            complement(2806..3168)
                     /locus_tag="VFQ61_09185"
     CDS             complement(2806..3168)
                     /locus_tag="VFQ61_09185"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9954667.1"
                     /translation="MALSESVTPVLVLIRTTLRPDCDVATYEAMDARMNELLREIPGY
                     LGVKGYTAEDGESVAIAEFASHEALVRWRDHPEHVAAQRAGRERFYASYDVRVCGVER
                     AYEFSMARVPQRLTKVGA"
     gene            3326..6127
                     /locus_tag="VFQ61_09190"
     CDS             3326..6127
                     /locus_tag="VFQ61_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954668.1"
                     /translation="MSKRLLGFVRSWLVRERRAALFGVASRETAAPRDSVAVELISVD
                     HLPRGLSLPPGLGSSWAALGHVRGIDAQVLADVLTVLLETLPDPQARPKRQANLEAAP
                     APPRATAAHPRGYRGTKSRPPKPALPERLDLRGDLISASDVARQLIALESYLPEALAL
                     LEGRGYSKERLTRTRLDANLYLAYHHGAERGDTLGSLPTDFVRWVLPALRRVGESSSA
                     VAGSVDVTMQQVRDWLTLWRLFEGKGEIQSWVSVLAQRAPSCATWLTLLLLSLPDERR
                     ELGLHALSGLDLKSLTGAIPEDALAELLRRFETLSSAQDADTYTARLRYLLNTAAQRG
                     AVELCLDAFALANTWHPSAVPKKSPRDASARAVDLEAYLAYADVGSDVTFGYWLFEHC
                     GELPGLARLIRDTAWKSFSPQTAYTLGCLWRGAAWEDISEERLLEKWGFIASEIPRSL
                     AWLSSVPEAYRERASTLLFEFLLGPWHEPQELAARRAAAYAIANRLASAPFSTHNRNA
                     WIVVPLVRHLHARPLHLDRFLAAPEATWAALDAALRRHNEGRLTIRGLDKLMKYLPEF
                     TIGALARHPAQLLSAAHTVGALHYDYADRFVQAFSAHPLLVLLAHESNEGDASPTPAA
                     DAEALVRALLPYVEQVGFKSNPVPRRLRESFATGSASQLSAGQLARHIRVLRVGLEAF
                     CVTFLRELVLKELRRGLPLDTEQDPVRHALLIERSAELNKRPLRRLIEAWADGNTEYL
                     SRHPATQAFRRRHSRLDARTWELWLNGIVLEGRVSTHGTEWIEGRGAQQVPLDGSVEG
                     QVLELSVERSPLEVLKLGTYVGSCLGLGGSFSFSAAAAALDINKRVIYARDPQGVVVA
                     RQLVALSEDDRLVCFHVYPLGLPAPLRELFRRYDEAWSAELGLPLFVRESSDRDYEIA
                     PILSRDWWDDGAWDFEIAD"
     gene            complement(6563..6814)
                     /locus_tag="VFQ61_09195"
     CDS             complement(6563..6814)
                     /locus_tag="VFQ61_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954669.1"
                     /translation="MDGLRVLSCVEATGAGRRSAIRGPPGVDVGLSYEGWLLSGTSDG
                     GSGLLESRDVSARCVDGSACKAFISPSAAAMICAWMWWV"
     gene            complement(6976..8388)
                     /locus_tag="VFQ61_09200"
     CDS             complement(6976..8388)
                     /locus_tag="VFQ61_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HET9954670.1"
                     /translation="MNAPSIDRDWGSVSPFVPPVGLSDGELLSATRRLVGRSNELLAL
                     LLGHLAEVESRGLHRTRACSSLYTYCIYELRFSEDEAYRRVAAARLVRRFPALLDAVA
                     SGELHLTGLLMLGPHLTQENLTEVLARAKHRTKKEIARLVRQLDPLPDVPARIEPLGP
                     APSRVLSATPTHIQFVESLCPVRELTSSDRPREWAGRAELTESAELTVSTEADSGECS
                     SEAAPGVDALPAPARLEAQRYRVEFTASEEYVQLVEAAQSLLSHSTERVPLAELQLRA
                     MRALVTELEGTRRAVTKQPVGTTASATEAATHAAQRPRADAPVPPEAPLAERRPRADA
                     RADSGPRASATQSTDAGSPRNVGQRVPSRPNEVRERADAGPEHPRQRVRYIPAAVRRA
                     VFERDQGRCAFIDGSGQRCRETHRLELHHVKAFARGGEHTVENLALRCQAHNALAAEQ
                     DFGADFIQQLRRHECCRHQA"
BASE COUNT         1485 a         2879 c         2745 g         1618 t
ORIGIN      
        1 ggcgcgcgac gcgagcggca ttgatctctg gagtctgcgt gccaacgaaa aagatccgca
       61 cgaagtatcc tgcacagatg gctcgtcgaa cgaattccgg tttgtctggg gcggagaaca
      121 ccgtctcgaa ggcaaggctc tttctctctc ggaggcaggt ctcgcggcgc tcctcagcaa
      181 cctgcgcggc ccgaagcaca gtctgcggat cattccaatc tccgagctcc tcttgcgcga
      241 tgacgtcggg attgacgtac tcgcagccgc tgaaccattc atgcgcaagc cctctctctg
      301 tgatcgtggt tttcggcacc gttcggacca gcgacaacaa gcaaccgggg ccgatatggc
      361 gtcggtgcat tcatggctct ggatcgccct ttctatcgcg agcgttctcg cgtgcgtagc
      421 gcgccgcttc ctcgagccga gcagcgatcc aagcatccga ttttgcccgc ctctggacag
      481 caagctctct ggcctcgcgc atcactagcg ccaattcttc atctgtgggc tctgtgtcgg
      541 gtgccatgag gtcgcggtcg gacatgatgg tttggcgtta gtttgaactg ggatgccgga
      601 taagcaatgt cggttccgga aggagtggta aggggccgct cagacagcca gcgcctagcg
      661 gaggcttggc gtttcctcct cgcgagcact ggatctctat gcagcctgat tcagtccacg
      721 tgcacgaacg gcgcgagcgg ctgcgctgtc tccctggcac ctgcgccaat caacgtgaga
      781 acgccggttg gggccgccgg attgtgagcc ggtgatggag aagccgcccg tgtccgcgat
      841 gccatcgaag tggcggctcc gtcggtgccg cgcgagccat cgtgaacgat cgctcttgcc
      901 cagcaatagt gaccttcagt tcgatactct tgagtacgtt gacagttggc tcggattgac
      961 ggcaaccggg atccggagcc gtcggtcaga gccagactgg cgagtgctgt ttcgactata
     1021 gccccgagcg gctggcggct cgcgagcacg ggtcccgtaa cccatcccta ttagcaaaat
     1081 gcatggcgac ggataactga actcgattgg acaccgttgc ggccccaatc taggctgcac
     1141 gacatgggat caatgaacct cgccaatgcg gggaagtacg ggagtttctt gcttttttgc
     1201 gtgcaactcg gctgcggccg gcagctgagc ccaacccagg gttggcccgt ggaggctggc
     1261 gccacgagta cggccggatc cggcggcacg ggcggcggcg tagcgcagca cggagggcaa
     1321 acgtcggggc tcgggggttc gggtcccgct tgggcacccg acgcgcgtct cgtagagagc
     1381 ggcactcgac tcaaagccgt gctctatacg ggcggcggga ccgaagtatt ccggcacttc
     1441 cacgacgaga agctgggctt tgactgcgag ttcgtcgagg ccgcgaacgg cgacgtgcgc
     1501 tgtgtgccgt cgacggtcgc ggtactgagt tatctcgatg cgagctgtcg agagccggcc
     1561 acatttctcg actttggtca tcttcccaaa gaaggcgact gggtccgggc cgacgcctgg
     1621 ggttccgctg ggagttgtcc gttctcgctc cccgatttcg ccgagaccta tcgcatcggt
     1681 gagcgcgttt atgatgagca ggtcccgggc aatgaaaggc agctcgtcta tggaatggtc
     1741 gactcccagt gccaagtcgt gacttcgagg cacggcaaga tcgacccgcc cgtgcatcgc
     1801 ttgattccgg tccctcgcga ggaactcgtc tcggcgcggc gccagcggtg ggttgcgtcg
     1861 gagcaattca acgtggtgag tttgcaagct gaggacggcg cttcgttgac gttaggggtg
     1921 gagacgcacg acggggctcc ttgcgccctc caacgcgacg gacgctgcgt cccgcacgtc
     1981 cccgccatca tggaagacgt gcgcgccccc tattctcacg cgctggattc gagctgcagt
     2041 cagcccgcct acgcgatccc catcgctgaa cgctgcggca ctccagagta cgcagtgcgc
     2101 ttcagcgaga ccggtcaaat ccgcgtgcac gagctcgccc ccgcgaacac cgtctactcc
     2161 ctacttcccg gcagattcgg gaccgaatac tgcaccgcgg tggtcgaggc gcccgggacc
     2221 tacttcggcc tcggcgcgga cgtcacggaa acgctgccgc aggcgatccc cgtgcggctc
     2281 ggcgaaggcc cgttgtggac ggagtctttc accgcgagca agggccttcc tccccttgga
     2341 acggggcgac cgctccaaca gcaaaccttc atcgacgcga ccggcgcgcc ttgccaaccg
     2401 gtgctcgcga tggactcgac tttgcgctgc ctgcgcaccc aaaacgcggt gctgcagtcc
     2461 agcttctggg cggacgcgga ctgcacactc cgcctgagca gcctggtatt gagcgctgac
     2521 accaacactc gagatctcct cgaacccgag tacaccgccg acggcaaact ctccggctat
     2581 cacgatctcg tgccgtactc tggccccatc tacgaggcat cctccgggag ctgcctccgc
     2641 gccgatccac ccgagccaca cttctatctg cccaaccgca ggtcaggttt cgaaactcta
     2701 cccgtcatcg aagaagtgcg ccgcgagtga ccctcgcgcc cggcttgctt ctacggccgt
     2761 agacctcaga aagaattgct gggcgccgag gcgggcgcgc gggcttcacg ctccgacttt
     2821 ggtcagacgt tgcggcacgc gcgccatcga gaactcatag gcgcgctcga caccgcaaac
     2881 gcgcacgtca taagacgcat aaaacctctc gcgcccagcg cgttgcgccg ccacgtgctc
     2941 ggggtggtca cgccaccgaa ccagcgcctc gtgcgacgcg aactcggcaa tcgccacgct
     3001 ctccccgtcc tcggcggtat aaccttttac cccgagatac cctggaatct cgcgcagcag
     3061 ctcattcatg cgtgcgtcca tggcctcgta agtcgccacg tcgcagtcgg ggcgcagcgt
     3121 ggtgcgaatc aacacgagaa ccggggtgac tgattccgag agagccatcg tcccggcggt
     3181 acgctcgccc cccctagcgg ttacaccggg gtgcgttttt tggttctaga ccgcgacccg
     3241 cgacgcgagc tgcgcccgca gatcatgagc ggtccagcca tgcagggcag gcgcgcgctt
     3301 cggcgccatg ccaaggtagg aggctgtgag caaacgcctg cttgggttcg tacgcagctg
     3361 gctcgtgcgc gagaggcgcg cggcgctctt tggagtggct agccgcgaga cagcggcgcc
     3421 tcgcgactcg gtggcggtcg agctcatctc ggtcgatcac ttgccgcgcg gtctttcgct
     3481 gcccccaggc ctcggttcgt cctgggctgc gctcggccat gtacggggaa tcgacgccca
     3541 ggtcctggcg gatgtactga cggtgttact cgagacgctg ccggatccgc aagcgcggcc
     3601 caaacgccag gccaatctcg aagcggcccc ggcgccgccg cgcgccaccg ccgctcaccc
     3661 gcgggggtat cgcggaacca aatcgcgacc acccaagccc gcgctgcccg agaggctaga
     3721 cctacgaggg gatctgatat ccgcgagcga cgtggctcgg cagctcatcg cgctcgaaag
     3781 ctatttgccg gaggcgctgg cactgctcga gggccgaggc tactcgaagg agcggctaac
     3841 gcgcactcgg ctggatgcga atctgtacct ggcctatcac catggggccg agcggggtga
     3901 cacgctcggc tcactcccca cggatttcgt gcgttgggtc ctgcccgcgc tgcgcagagt
     3961 gggtgagagc tcgagtgcgg tggcgggctc ggtcgatgtt acgatgcaac aggtccgaga
     4021 ttggctgacg ctctggcggt tgttcgaggg caagggggag attcagtctt gggtatccgt
     4081 cttggcgcaa cgcgcgccat cctgtgcgac gtggctcaca cttctactgc tcagcctgcc
     4141 tgacgaacgt cgagagctcg gcctccacgc gctctcgggt ctggacttga agtcgctcac
     4201 gggcgcgatc ccggaggacg ctctcgccga actgctgcgg cgcttcgaga ctctgagctc
     4261 cgcacaagac gccgacacgt acaccgcgcg cttgcgctac ctgctgaata cagccgctca
     4321 gcgcggcgcc gtcgagttgt gcttggacgc cttcgcgctc gcgaacactt ggcaccctag
     4381 cgccgttccg aagaagtcgc cgcgtgacgc aagcgcgcgc gccgtcgatt tggaagccta
     4441 cctagcttac gcggacgttg ggagcgatgt cactttcggg tactggctgt tcgagcattg
     4501 tggtgaactt ccagggctcg cgcgcctgat tcgggatacc gcttggaagt cattttcacc
     4561 ccagaccgcg tacaccctcg gctgcttgtg gagaggggcg gcttgggagg atatatcgga
     4621 agagcgatta ctcgagaagt gggggttcat cgcttcggaa atcccacgct cgctcgcttg
     4681 gctgagctcg gttcccgagg cataccgcga gcgcgccagc actttattgt tcgagtttct
     4741 attgggacct tggcatgaac cgcaggagct cgccgcgcgg cgagcggctg cgtatgccat
     4801 tgccaaccgg ctcgcgtcag ccccgttttc gacccacaac cggaacgcct ggattgtggt
     4861 gccgctcgtc cgccatctcc acgccagacc gctgcatctg gatcggtttc tggcggcgcc
     4921 cgaggcgact tgggcggcgc tcgacgccgc gctccggcgc cacaacgagg gccgactaac
     4981 gattcgaggc ctggacaagc tgatgaagta tctgcccgag ttcacgatcg gcgccttggc
     5041 tcgtcatccg gcgcagttgc tcagcgcggc tcacaccgta ggcgcgctgc actatgacta
     5101 cgccgatcgg ttcgtgcagg ccttctccgc gcacccgctc ttggtcttac tcgcgcacga
     5161 gagcaacgag ggtgatgcct cgccaacgcc tgcggccgac gcggaggctc tggttcgcgc
     5221 actgctcccc tacgtcgagc aagtaggctt caagtcgaat ccggttccgc gtcgtttgcg
     5281 cgagagcttc gcgaccggga gcgcttcgca gctgagcgcg gggcagctcg cgcggcatat
     5341 ccgcgtcctg cgcgtgggtc tcgaggcctt ttgcgtgacg tttttgcgag agctggtttt
     5401 gaaggagctg aggcggggtt tgccgctcga taccgagcaa gatccggtac gtcatgcgct
     5461 actcatcgag cgctccgccg agttgaacaa gcgcccactc cgccgcctga ttgaagcttg
     5521 ggcagacgga aacacagagt atctgagtcg gcaccccgcc acgcaagcgt tccggcgtcg
     5581 tcattcgagg ctcgatgcgc gcacgtggga gctctggctc aacggcatcg tgctggaggg
     5641 cagggtctcg acacacggca cggaatggat agagggcaga ggggcgcagc aagtgccgct
     5701 cgacggcagt gtggaaggcc aggtgctcga gctctcggtc gagcgctcac ccttggaggt
     5761 gctcaagcta ggaacgtacg tgggaagctg tctgggcctg ggcggctcgt tttccttctc
     5821 cgccgcggcc gcggccctgg atatcaacaa gcgcgtgatc tacgcccgcg acccgcaagg
     5881 cgtcgtcgta gcgcgccagc tcgtggccct gtcggaggac gaccgcctcg tctgttttca
     5941 cgtctacccc ctcgggctcc ccgcgcctct acgcgagctg ttccgccgtt atgacgaggc
     6001 atggagcgcg gagctcgggc tgccgctgtt tgttcgcgag agctccgacc gcgactatga
     6061 gatcgccccg attctctcgc gcgactggtg ggacgacgga gcttgggatt tcgagatcgc
     6121 ggactagatg cggggcacgt cttcacttcc ctcgtcgatc tcccccgcct atcccaagcc
     6181 actaaacccg aggctccgtc atttcccgcc gatccttcga ggtaaaggcc cttgtagagc
     6241 tcaccggcct cgcgaaatcg agcaccgcgt ggacgcgctg caggcggggt atcgcacctc
     6301 gcccgcgaag ctctctctcc cgaagcaagt ccaccaaccg cggtgcgcat catccgcctc
     6361 gcagcgtgcc ggaccctcaa tcccacgtgc cttcagctcc ggtgtcagct cttccagaag
     6421 cacgggcccc cgcggcgccg gcaaaccggc cttgaggcgc ggtagctcgg gcgccggaac
     6481 gaactgcaac tgaatcgtga aacgcgccag tcatcttgat cggggtttcg atagcggagc
     6541 tcgacatccg cgggcccggg cttcagaccc accacatcca cgcgcaaatc atggcagcag
     6601 cagaaggaga gatgaacgcc ttgcacgccg acccatccac acagcgcgcg ctcacgtcac
     6661 gtgattccaa aagacccgag ccgccatccg acgtcccact cagcagccaa ccttcgtagc
     6721 tgagccccac gtctaccccg ggcgggcccc ggatggcgct cctgcgaccg gcgcccgtgg
     6781 cttcgacgca ggatagtact cgtagtccat ccaccctccg ggcacgttca acgcatcgag
     6841 ccggcccgcg ctgtcgtagc tgtacccgag ctcacgttct ccggggcggc tctccgtcaa
     6901 aggcatgcgg ttcggcgtct gagtatggct cgtcgtggcc ccatcgttga gaattcgctg
     6961 accacgctcc cgtcctcatg cctgatgtct gcaacattca tgtcggcgaa gctgctggat
     7021 gaagtcggca ccgaagtcct gctcggctgc aagagcattg tgcgcttggc aacggagggc
     7081 aagattctcc accgtgtgct cgccgccccg tgcgaaggct ttcacgtgat ggagctcgag
     7141 ccgatgcgtc tctcggcaac gctgtcctga gccatcgatg aacgcgcagc gcccttggtc
     7201 gcgctcgaag acggcgcgcc gaacggccgc agggatatat ctcacgcgct ggcgcgggtg
     7261 ctccggcccg gcgtcagcac gctcccgaac ttcgttcggt ctggaaggaa ctcgttgccc
     7321 cacgttccgc ggcgaccccg cgtcggtgct ctgtgtcgca ctcgctcggg ggcctgagtc
     7381 cgctcttgca tctgctcgag gccgccgctc cgcgagcggt gcttccggag gcactggcgc
     7441 gtctgctcga ggtcgttgcg ccgcgtgcgt tgcggcctcg gttgccgacg cagtggtccc
     7501 taccggctgc ttcgttacag cgcgcctcgt tccttcgagt tcggtcacga gagcgcgcat
     7561 cgcgcggagc tggagctcag cgaggggaac gcgctccgta gagtgcgaga gcagcgactg
     7621 ggccgcctcg acgagctgga cgtactcctc gctcgcggta aactcgacgc gatagcgctg
     7681 agcttccagc cgcgctggcg cgggcaacgc atccacgccc ggtgccgcct cgctcgagca
     7741 ttcgcctgaa tctgcctccg tgctcacggt gagctctgca ctttcggtga gctccgcgcg
     7801 cccggcccat tcgcgaggcc ggtcacttga ggtcagttcg cgaaccggac acagcgattc
     7861 gacaaattgg atatgggtcg gcgtcgccga aagcacccgc gatggcgcgg gaccgagcgg
     7921 ctcgatgcga gccggaacgt cgggcagtgg gtcgagttgt cgaaccaacc gcgcgatctc
     7981 tttcttggtc cgatgcttgg ctcgggccag cacctcggtc aggttctcct gggtcagatg
     8041 cgggccgagc atcaacaagc ccgtgagatg cagctcgccg gaggcgaccg cgtcgagtaa
     8101 agccggaaag cggcgaacca accgtgccgc cgccacacgt cgataggctt cgtcctctga
     8161 aaaccgcagc tcgtaaatgc agtacgtata gagactcgag caagctcgcg tgcggtggag
     8221 ccctcgagac tccacctcgg cgaggtgacc gagcagcagt gccagcagtt cgttggagcg
     8281 gccgacgaga cgtcgcgtcg cgctgagcaa ctcgccgtca gagagcccga cgggtggcac
     8341 gaacggagac actgaacccc aatcccgatc gatactcggc gcgttcatga gtcatacctt
     8401 acaccctgat tttcggagct ccctattggc accctcgcgc atcttcgcgc gcagacacac
     8461 cgtgcaagct gtctgcccgc gcgaaagcag cacatcgggc cgtgtgctcg cggcaaacgc
     8521 gactaaaccc ctgtcaacgt gccgatcgac accgacccgt tcggaatcgt gtcaagtccc
     8581 gaatgcaaat ccacgcttcg ggatccagta tctcagcgcg cgatctgtcg aagacggcgg
     8641 ccatctactt cgctgagtct tcagtaggcg gcgggtatca aatctatcct tcgcgtagtc
     8701 cgcaccttga tctccatgca gttcgca
//
LOCUS       DASTKU010000254        10393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_718673, whole genome shotgun sequence.
ACCESSION   DASTKU010000254 DASTKU010000000
VERSION     DASTKU010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10393
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718673"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFQ61_09205"
     CDS             <1..207
                     /locus_tag="VFQ61_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954671.1"
                     /translation="WGRREQIRRVCEAINASRGESERRFYEYAEDLPGWPAYFEPLWL
                     WLSAWEDEALRRLGIVAPAGGNSG"
     gene            complement(297..1091)
                     /locus_tag="VFQ61_09210"
     CDS             complement(297..1091)
                     /locus_tag="VFQ61_09210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9954672.1"
                     /translation="MTQSAYIFQGTPDAAELARLQMLEAVFDDPSQRCIRSVGPLLGS
                     RCLEVGAGAGSIAAWLASEVGASGAVVAVDTNTRFLGGLPREVQVIEGELSALALPAG
                     SFDLVHSRYVLIHNANTDEILDAMFKALKPGGALILEEPDFSSARAFTGPPPLRQAFD
                     NVQKAIRETFSARGMDHAFGSALPELLEKRGAKLRTLEYDCPVASGGSRLAEMMRLST
                     IALQEKYLATGLVTPTDIANYGEFAKKTSCWANYYATVRIGAEKGL"
     gene            complement(1136..1465)
                     /locus_tag="VFQ61_09215"
     CDS             complement(1136..1465)
                     /locus_tag="VFQ61_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954673.1"
                     /translation="MTSPGGGNTECEGRERKTAWRRALLRWEIRLAVPLGLLGVLLTS
                     AALVFGPGDPHHGGTHAFLLGLTALLVAAVLGLAAVGSLGMRVIGWVLKSALVAILAW
                     ILWNLIS"
     gene            complement(2325..2450)
                     /locus_tag="VFQ61_09220"
     CDS             complement(2325..2450)
                     /locus_tag="VFQ61_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954674.1"
                     /translation="MTKRLQVIGNGKGIAIAERRLDKPSDATLFAKLAMRDEQAL"
     gene            2887..3369
                     /locus_tag="VFQ61_09225"
     CDS             2887..3369
                     /locus_tag="VFQ61_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954675.1"
                     /translation="MIRRSIGRVNALRGQQFRVVEFSVQANHIHLIVEAEGRAALSCG
                     MRSLNARIGKQVNRVLARRGRLISDRHHEQVLRCPRAVRHALVYVIANFRKHPTQEGR
                     RGELTDPCSSALFFDGFAEVRGTERTLDSPVIEARTWLLRAGWRRHGLISIYEAPRSA
                     "
     gene            complement(3546..5072)
                     /locus_tag="VFQ61_09230"
     CDS             complement(3546..5072)
                     /locus_tag="VFQ61_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HET9954676.1"
                     /translation="MAQTEPVILGFMGNRGFFPTQFAEEGARATQSAVSRVLGERVRY
                     VDLGNVETYGDARAAAGVAQQYRRNPDGGGAIGIICSMYNFSDENGIRDFLRLADLDI
                     PVLLHTEPDVRGLGRMGQKGRRDGACGRFSAANALRHIGYPYTLTSKHCESVSSAAFA
                     EDLQAFYASCVLASKFRRRGRGVRLGLVGSGPDAFQTVTNVSTELLGHMGLSTIGLEL
                     IALDRQMRQVSSRELATQLEAVSGYMAASDVPRASLEAIARMGVVFDRFVKENDLDGI
                     AVRCWTEMQKYQIGGEVGVMPCTCMSMLSDKLIPAACETDIAGWMGMYMLQCASGLVP
                     LLGDWNNMYDDEREEVDLFHCGVWAKSVMRPGARITRQEILATQADIGAENTWGAVDG
                     ELKAGTCAFLRPCTNAREGSVFLYGGVGRVSEEGIDTFGTRGRIQIPRIQQLFRHLTQ
                     PERAVEHHTALVVGTQKNIELTMKAIRDAVPYINAQAGLGRNGQALIDFYEHSQVSEL
                     "
     gene            complement(5310..6383)
                     /locus_tag="VFQ61_09235"
     CDS             complement(5310..6383)
                     /locus_tag="VFQ61_09235"
                     /inference="COORDINATES: protein motif:HMM:NF025746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4419 domain-containing protein"
                     /protein_id="HET9954677.1"
                     /translation="MTTFSLSAVSRGPLYPPLDVEASVRNLLKKPVEAMGCGTQRLVS
                     CDTTHAFVKAAYDAFYRHYPLTIRPDDVWLCIAQGFAAHVNHNAEELRSRFVAHSGKK
                     KLVVERPDFFLGQANPWPEAFCAFSEQIGAHVGKLRELVCARFSTTTPIEEAAFDVCL
                     MDTFQGYFEYEMLAGCGIPEITVLGTPEDWRSMIPRVKHLSEYGLNTWCDSLVPVLEK
                     IADTAAGKVDREFWLSFFRYQSGSGPAELTGWILTLFPYLVTDWQTKALGPNPYLSTW
                     KARFDHAMNRTGYIRFDDVQGPSIALFPEGLVSAPVHYVDRSRDRDYDLRFVAGMFGV
                     EQDVETRALSASFGWAVVYDDAK"
     gene            complement(6427..6990)
                     /locus_tag="VFQ61_09240"
     CDS             complement(6427..6990)
                     /locus_tag="VFQ61_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9954678.1"
                     /translation="MSKTAKEPQLRAIVVEVIDPAGKPLVNAEVEGLVDVNPGPVAST
                     CCITKHFASAKTSAEGRATLDGAPYTFRAFHFRASFQGWPRRETTASLSLGQPNVVRI
                     QLGPARAVRGRVDLGPDCPLPSDLDVRASASTASARVAADGSFFFKSLPPWSTLSINA
                     CGRSATAALEIGDDRPVLLTLPGAQSP"
     gene            complement(7181..7942)
                     /locus_tag="VFQ61_09245"
     CDS             complement(7181..7942)
                     /locus_tag="VFQ61_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954679.1"
                     /translation="MVLLPGFACGGKVHESPPPESGEHQAGDANTLATGGTIAVGGAS
                     AIAVGGAVSSVGGFVTGGTLSMGGTVWTGGVSTGGKMVALPADKALGALTADEVEQFR
                     GEIVSLIDERMAAVPLDVRCHYLGVVQASLIAGAGASGTSDDVRESCALSEADCVAGK
                     RSVYIEPRLLHPTNTENEDGFRECSATVGDYRTCTADLVQAKADVMVALPACSALDVK
                     YYHGVLGDVPVKPTEPSSCSSLYSECWRILAHRPE"
     gene            complement(8116..8442)
                     /locus_tag="VFQ61_09250"
     CDS             complement(8116..8442)
                     /locus_tag="VFQ61_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9954680.1"
                     /translation="MTWHPSSPAVSLKEIECAECLVLHCWAEWNEYDRVLDSRLSAAS
                     TEFRACAAFRAADVDEPSLAATLHAWNIVNVPALLLLRQGRKIAVHCGLLSEEDLASL
                     LHRWLR"
     gene            complement(8486..8887)
                     /locus_tag="VFQ61_09255"
     CDS             complement(8486..8887)
                     /locus_tag="VFQ61_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954681.1"
                     /translation="MKLLIFGWLAVMVATIATAAVARLNRGHHGVAAWKGSLRACGFA
                     AFPGIVLFSEFVAHAVLSKASRIPGPAVYDYWGTLLSVLGIFGGPLFFGAAYYRRPGG
                     RVPMRVGVLVFLCFWAWVTSSLTALVFALSV"
     gene            complement(9006..9377)
                     /locus_tag="VFQ61_09260"
     CDS             complement(9006..9377)
                     /locus_tag="VFQ61_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954682.1"
                     /translation="MQTIKLILTILSLIAGGSVLALVLMAAGLTLIARRALLSAIRAL
                     GCPFCSASLSEHSIELADELWGRHMSALAQQNPGVRFRIVRPLDVVCESCGARLQYVK
                     ETRSLVPISIVLAFEAPNVKS"
     gene            complement(9411..10325)
                     /locus_tag="VFQ61_09265"
     CDS             complement(9411..10325)
                     /locus_tag="VFQ61_09265"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET9954683.1"
                     /translation="MGAVLGLALGDAFGAPFEGGPLERALWRLIGTTYDGRLRWTDDT
                     QMSLDLAESLVASRAVNQDDIARRFARGYRWSRGYGPGAARILKLIRRGVDWRSANTR
                     FFRDGSFGNGGAMRAPIVGLFSCSDLAAVQARARATAEVTHAHPLGQEGAVLVALATA
                     LLLRSDESRRVLDALAAIAEQDPFRERLAVAKSWLDNERPSPPQVRQHLGNGIAAPES
                     CITAIYVALRFLDRPFPELLEFVAALGGDVDTIGAIAGALWGARNGDASLPASMLERL
                     EDADRLRQMALALHRATLEGPSKFPSQS"
BASE COUNT         2153 a         3195 c         3097 g         1948 t
ORIGIN      
        1 tggggaaggc gtgagcagat ccgtcgcgtg tgtgaagcga tcaacgcttc acgaggcgag
       61 agcgagcggc gtttctacga gtacgcggaa gatttgccag gctggcccgc gtatttcgag
      121 ccgctgtggc tgtggctctc cgcgtgggaa gacgaggcgc tacgtcggtt gggtatcgtg
      181 gcgcccgctg gcggaaattc tggctagacc tcgaacatgg acgcaggcga accactggaa
      241 caaaaacgct ggaccgcgca cgaattctta gcgtgtccat gcaccaccct gctgactcaa
      301 agaccctttt ctgctccgat tcgcaccgtg gcatagtagt tcgcccagca actggtcttc
      361 ttggcgaact ctccgtaatt ggcaatatca gtaggcgtga cgaggccagt cgcgaggtac
      421 ttctcttgaa gagcaatcgt tgacaaacgc atcatttcgg caagccgtga gccaccgctt
      481 gcgaccgggc aatcgtactc cagagttctg agtttcgcgc cgcgtttttc gagtagctcg
      541 ggcaaggcag agccgaaagc gtggtccatg cctcgcgcag aaaacgtctc gcgaatggct
      601 ttttgcacgt tgtcgaacgc ttgcctgagc ggcggaggcc ctgtgaaagc tctggccgag
      661 ctgaaatccg gctcctcgag gatcagggcc ccaccgggct tcagcgcctt gaacatggca
      721 tcgagaatct catccgtgtt cgcgttgtgg atcagaacat aacgagagtg aacgagatcg
      781 aaagaaccag ccggtagcgc caacgcgctg agctctcctt cgatcacctg cacctcgcgc
      841 ggcaggcctc cgaggaagcg cgtgttcgta tcgaccgcga ccacggcgcc gctcgccccg
      901 acttcggagg ctaaccacgc tgcgatcgag cctgcgccag cgccaacctc gaggcatcgg
      961 ctgccgagca gtggtccgac cgagcgaatg cagcgttgac tcggatcgtc gaacaccgcc
     1021 tccaacatct gtagtcgcgc gagttcagcc gcatccggag tgccctggaa tatgtacgca
     1081 ctctgagtca tttctcccga taccttccgg atctccttcg agttcgagca ctcgactatg
     1141 agataaggtt ccaaaggatc caagcgagaa ttgcgacgag ggctgacttg aggacccagc
     1201 ctatcactcg catcccaagc gaaccgacgg ccgccaaccc gagtactgct gctacgagca
     1261 gcgcggtcaa gcctagtaga aaggcatggg tgccaccatg atgcggatct cccggaccga
     1321 agacgagggc cgccgaggtg agtaacaccc cgagcaatcc gagcggcacg gcgagccgaa
     1381 tctcccatct taggagggct cgacgccacg ccgtcttgcg ctcacggccc tcgcattctg
     1441 tgttgccgcc gcctggtgag gtcatgtttt gaccggcacg cgcctctcgc acgcgccaaa
     1501 ggatccgaac acgactggac tgttttcgcg atcgagacgt gcacgccaaa aggttacgcc
     1561 gctcatcgct ttgagcaatt gactatgtgg tggcacgggg cagctgttcc agcaggttgg
     1621 gacccgttcg aacgactggg cactcccttc ctcatatgcc attagaatga gcttcgttct
     1681 atcacaggcg cggcaggctc gcccggtcga tgagctctca gcgtaacaac ccagcgattt
     1741 tccggtgcag acctcgacgg gaagcacgtg ttctccaccg tattcgagct cacgctcgtt
     1801 ggcagcggtc aatagaccat agtttttgcg cagcgaagac gcaacggcaa ccccctccct
     1861 gcgacgccac gttcctccaa tctcctcatc gtagcgaatc accgcgctat cgaccactag
     1921 gtccggactg agttcgcacc tggaactctg acgatagtgt ttgccatctc ttgtctcggt
     1981 tccggtgacg agccagcaag tatcaccgta tgcgtcaggg ggcccgacaa gcagcgagaa
     2041 acaactcgcg ctcacgggca ggtgcacagc atactcttgc tcgtcgtgcg gttggagcca
     2101 gcccggcaat tgtactttgc tgaccctcac gttgcgcatt tcgtcaggcc acagacgtcg
     2161 tagttgggca acgctcatgt ctgccccggc gccgtcgcca gcgggcgagc ctagctgcgc
     2221 cggactcgga gtagccgcac aactcaaagt gacgacggca gcaagcagcg ctctgctggg
     2281 cttggctacg ctcggcttaa gccggagcag gaggcgtttg tcggtcacag cgcttgctca
     2341 tcacgcattg cgagcttcgc gaacagggtt gcatcgctgg gcttatcgag acgacgctcg
     2401 gcgattgcga ttccctttcc gttaccgatg acttggagtc gtttcgtcat gctgagttcg
     2461 tcaggcctgg cccgttgcgg ccgaggccgg cttggcgttg atgtacggca cggcatcgca
     2521 caagggtatc agcgactcgt ctcctcgtct tgattcggcg atgttcgaca cgagagtgct
     2581 cactcttaca gacacaaatg cattgtcaaa agggatgcct gatggcaaag tgagcgcgtc
     2641 agccgacgag cgcgcgagtc tagaaaagaa agaggggttc gagctatgtg catatggaag
     2701 gttccgcggg agcagctgag cttggatttc cagagtacgt ggggcgggaa acgctcgggt
     2761 gccgggcgca agcgtcgggg caattcgatg ccgcatgcgc ggcgcgacgc tcatcgacga
     2821 tatgagccag ttcacgtcac cgtacgctta ggggtgcggt gtttgagacg gcaagtcgtg
     2881 ttcgccgtga ttcggcggtc gatcggtcga gtgaacgcgc tccgggggca gcaattccgc
     2941 gtggtggagt tttcggtgca ggccaatcat attcacctga tcgtggaggc ggaggggcgc
     3001 gctgcgttga gttgcggcat gcggtcgctg aacgcacgaa ttggcaagca ggtgaatcga
     3061 gtgctggcgc ggcgggggcg attgatctcc gatcgtcatc acgaacaggt gttgcgctgt
     3121 ccgcgtgcag tgcgccacgc actggtttat gtgatcgcga acttcaggaa gcatccaacg
     3181 caagagggcc ggcgtgggga gctcacagat ccatgttcct cggccctctt cttcgatggt
     3241 ttcgcagagg tgcggggcac ggagcggact ctggactctc cagtcatcga ggcacgtacc
     3301 tggttgctgc gggctggctg gcggaggcac ggcctcatct cgatctacga agcaccgcgg
     3361 agcgcataag cggtgcgcgc cccgctaaag ggatgggttc agtcgctgat gacgatgtag
     3421 gcggtgttca cgtcggctcg cgtaacatcg attagggtgc tgaacgccca tgggccgtgt
     3481 cgcccctcgc agccccggtg acgctttacg gtctcgggcg tgtcccagct acgatcagag
     3541 caagctcaga gctcactcac ctgactgtgt tcgtagaagt caatcagggc ctggccgttg
     3601 cggccgaggc cggcttgcgc gttgatgtac gggacggcgt cacgaatggc tttcatggtc
     3661 agctcgatgt ttttctgggt cccgacgacg agggcagtat ggtgttccac ggcgcgctcg
     3721 ggctgcgtca ggtggcgaaa gagctgctga atgcgaggga tctgaatgcg accacgggtg
     3781 ccgaaggtgt ctataccttc ttcactgacg cgacctaccc cgccgtaaag gaatacgctg
     3841 ccttcgcgcg cgttggtgca aggccggaga aaagcgcagg tacccgcttt gagctcgccg
     3901 tcgaccgcgc cccaggtgtt ctcggcgccg atgtcggctt gcgtcgcgag gatttcttgg
     3961 cgggtgatac gggcgccagg gcgcatcacg ctcttggccc aaacgccgca atgaaagaga
     4021 tcgacctcct cgcgctcgtc atcatacata ttgttccagt caccgaggag cggcacgagc
     4081 ccggaagcgc actggagcat gtacataccc atccagcccg caatgtcagt ttcgcaagcc
     4141 gcgggaatga gcttgtctga aagcatgctc atgcaggtgc acggcatcac ccccacttcg
     4201 ccgccaatct ggtacttctg catctccgtc caacaacgga ccgcgatccc gtccaggtca
     4261 ttttctttca cgaatcgatc gaagacgacg cccattcggg caattgcttc cagagaggca
     4321 cgcggaacat ccgacgccgc catgtagccg gagaccgctt cgagttgagt ggctagctca
     4381 cgcgacgaga cctgacgcat ctggcgatcc agagcgatca gctccagacc aatcgtgctc
     4441 aggcccatgt gccctagcag ctcggtagaa acattggtca ctgtctggaa tgcatccggg
     4501 cccgaaccaa ccagcccgag acgaacgccg cgtccgcgcc gacggaactt cgatgccaac
     4561 acgcagcttg cgtaaaaagc ctgtagatct tcggcaaatg ccgccgaact cacactttcg
     4621 caatgcttgg acgtgagggt gtacggataa ccaatgtggc ggagcgcgtt agccgccgag
     4681 aaacgtccgc atgcaccgtc gcgcctcccc ttctggccca tgcgacccaa tccgcgcacg
     4741 tcaggttcgg tgtgcagcag caccggtata tcgaggtcag ctagccttaa aaaatcccga
     4801 atcccattct catccgagaa gttatacatt gaacagatga tgccgattgc gccgccgccg
     4861 tcgggattcc ggcggtactg ctgggcaacg cccgcggccg cccgagcgtc cccgtaagtt
     4921 tcgacattgc cgagatcgac atagcgcacc cgttcgccga gcacacggct cactgctgat
     4981 tgagtggccc tcgcgccttc ctccgcaaac tgcgtaggaa agaagccgcg gttacccata
     5041 aaccccaaaa tcaccggttc agtctgtgcc attgtctctc ctccaaagca ggcgagccgc
     5101 cgcactctca acttccgttg agcatcacga agtttctgag ttagaggcgc gctcgactaa
     5161 cgcggcactg accgtaacag aggctgcggt gcgtcaggaa caaagaaagg aaagtatcct
     5221 caccaaacaa agaatagcca gatgagcgga atactctcgt gtcaccgtat ttctgtgacc
     5281 ggttggcgct cggcttggcg ctgccgagct cactttgcgt cgtcgtacac gaccgcccaa
     5341 ccgaacgaag cggagagcgc ccgtgtctcg acatcctgct cgacaccgaa catgccggcg
     5401 acaaagcgca agtcgtagtc acggtctcta ctgcgatcga cgtagtgcac cggagcgctg
     5461 accaggccct cggggaacaa cgcaatacta gggccctgca cgtcgtcgaa ccgaatgtag
     5521 ccagttcggt tcatggcatg atcgaaccga gctttccacg tagatagata cgggttcggg
     5581 cccagcgcct tagtttgcca gtccgtcacc aaatatggga atagcgtcaa gatccatcct
     5641 gtcagctcgg cgggccctga accactctgg tagcggaaga acgagagcca aaactctcga
     5701 tctactttgc cggcggccgt atcggcgatc ttctcgagta cgggcacgag cgagtcacac
     5761 cacgtgttca atccatattc cgagaggtgt ttcacccgcg ggatcatcga acgccagtcc
     5821 tctggtgttc cgagcaccgt aatctccgga atgccgcaac ccgccagcat ttcgtactcg
     5881 aagtagcctt gaaaggtgtc catgagacag acatcgaagg cggcctcttc gatgggcgtg
     5941 gtggtcgaga aacgagcaca gacgagctct ctcaacttac ccacgtgcgc cccgatttgt
     6001 tccgagaatg cacagaaagc ctcaggccat gggtttgcct ggcccagaaa gaagtccgga
     6061 cgttcgacga ccagcttctt cttgccgctg tgtgcaacga agcgtgaccg gagctcttcc
     6121 gcgttgtgat ttacgtgcgc ggcaaagcct tgcgcgatgc acagccacac atcgtccggg
     6181 cgaatcgtga gcggatagtg gcgatagaac gcatcatacg ccgctttcac gaaggcatga
     6241 gtcgtatcgc acgaaaccag ccgttgcgtc ccgcagccca tcgcctcgac gggcttcttg
     6301 agtagattac gcacactcgc ctctacgtct agcggcgggt aaagcgggcc tcgggagacg
     6361 gcggatagag aaaaggtcgt catgatctca tctcgtttct gtgtcgcgtg tcgggcgctt
     6421 cgcgactcat ggggactgcg ctccgggtag agtgagaagc acaggtcgat cgtcaccgat
     6481 ttccagcgcc gcagtggcac tgcggccaca ggcattgatc gagagtgtcg accacggagg
     6541 cagactcttg aagaagaagg atccatcggc agccactctg gcactcgctg tcgacgcgga
     6601 agctcgaaca tcgagatcac tcggcagggg gcagtctggt cccaagtcga cacgtcctcg
     6661 caccgcacgg gctggtccga gctggattcg aaccacgttg ggttggccta acgacaggct
     6721 cgcggtcgtt tcccttcgcg gccaaccctg aaacgaggcc cggaagtgaa acgccctaaa
     6781 cgtgtaaggg gctccatcca gagtcgcgcg gccctccgcg gaagtctttg cgctcgcgaa
     6841 gtgcttggtg atgcaacagg tcgatgcgac tggccccggg ttgacatcga cgaggccttc
     6901 cacctcggcg ttcacgagcg gtttacccgc tgggtcgatc acctccacga cgatggcccg
     6961 gagctgcggt tcttttgccg ttttcgacac acagccagcc agcaatccaa gggtcgcaag
     7021 cgacaggcct cggaacaccg ccgttgggcg ggttcgcgaa ctccgcctac tccgcaccca
     7081 accctcaccg tcaggcccgc tgacggctcg gtaagatcgt gagtttaatg acgacgcggc
     7141 gcatttggaa tgtccatgca tacagccact cgcagaggtt tcactcgggt cgatgcgcta
     7201 gaattctcca gcactccgag tagaggctcg agcagctcga aggttctgtc ggcttcacgg
     7261 gcacatcgcc gagaacaccg tgatagtact tcacatcgag cgcactgcac gcgggcagcg
     7321 ccaccatgac gtcggctttg gcttgaacca agtctgcggt gcaagttcgg taatcaccga
     7381 ctgtggcact acactcgcgg aagccatctt cgttttcggt gttcgtggga tgaagcagtc
     7441 gcggttcgat atacacagac ctcttgcccg cgacgcaatc ggcctcactc agggcgcacg
     7501 actccctcac atcgtcactt gttccgctcg cgcccgctcc agcaatcaaa gaggcttgca
     7561 ccacacccaa atagtggcac cgcacatcga gcggcacggc cgccatccgc tcgtcaatca
     7621 gcgacacgat ctcgcctcta aactgctcga cttcgtcagc ggtcagagcg ccgagagcct
     7681 tgtcagccgg gagagcgacc atcttccccc cggtcgacac accgccagtc cacaccgttc
     7741 cccccatcga tagcgttcct cccgtaacga acccgccgac actcgatacc gcccctccga
     7801 ccgcaatcgc gcttgcacct ccgaccgcga tggtcccccc cgttgcaagc gtgtttgcgt
     7861 cgcccgcctg atgctcaccg ctctcgggcg gtggactttc atgaactttt cccccgcaag
     7921 caaagcctgg aagtagaacc atggcaattg tcgcgaaccc tctgtagctc aagcccataa
     7981 tccttcctac cgctcgaatt ccagaatgtc caacgcgcac ggcaatcgag cattgttcac
     8041 ggggatcccc gtcgcgctcg gagtgctgaa gggcgtttac ggcatcgcct gtcaggggtc
     8101 atcgcaagtc ctgcgttacc tgagccagcg atgcagcaaa gacgccaagt cctcctcact
     8161 caacagaccg caatggacgg caatcttccg accttggcgc aatagcagga gcgcgggcac
     8221 gttgacgatg ttccacgcgt gcagcgtagc tgccagagac ggttcgtcga cgtcggctgc
     8281 gcggaacgcc gcacacgctc ggaattcagt actcgccgcc gacaaccgac tatcgagaac
     8341 tcgatcgtat tcgttccatt ccgcccagca gtgaagcacc aaacactccg cacactcgat
     8401 ctctttcaga gatacggctg gggaagaggg gtgccaggtc attcggtcat tcgtagggtg
     8461 ggagagcata ccatcgcgcc aaacttcaaa cactcagcgc gaaaaccagc gccgttaggg
     8521 aagacgtcac ccaggcccaa aagcagagga ataccagaac tccgactcgc atgggcacgc
     8581 gcccaccggg acggcgatag taggcagccc cgaagaacag agggcctcca aagatcccaa
     8641 gaacggaaag cagagtgccc caataatcgt aaacggccgg gcccggaatt ctggaagcct
     8701 tggataagac cgcgtgagca acgaactcgg agaacagcac aatcccggga aacgccgcga
     8761 agccacacgc ccggagtgaa cctttccaag cggcgacgcc gtggtgaccg cgattgagcc
     8821 gggcgacagc cgccgtggcg attgtcgcga ccatgaccgc gagccaaccg aagatcagaa
     8881 gcttcatggc gttcgtcgga gcgtacacgc gcggtccgag catgtccggg gaggcttgga
     8941 ggaagtactg aggagtcgag agatgtctgg ctcggccgcc tgccctcgtc agacgaccct
     9001 gaaccttagc tcttcacgtt gggcgcctcg aaggcgagga ctatcgagat gggcacgagt
     9061 gatcgggtct ccttgacgta ttggagtcgc gcgccgcagc tttcgcagac cacgtccagc
     9121 ggccttacga tgcgaaacct cacgccgggg ttctgctgag cgagagcgct catatgtcgc
     9181 ccccacagct cgtcggcgag ctcaatcgag tgttcggaaa gactggcaga gcagaaagga
     9241 cacccgagcg cacggattgc cgataagagt gccctgcgag cgatcagggt caatcccgcc
     9301 gccatcaaca ccagagccag aacagacccg cccgcgatga gcgaaaggat ggttaagatc
     9361 agcttaatcg tttgcacgga tttgccgctc gctcgtttat actcgtgctc tcacgattgg
     9421 ctggggaact tggacggacc ttccagggtc gctcggtgaa gagcgagggc catctggcgc
     9481 aaacggtcag cgtcttcaag gcgttcgagc atgctggccg gaagcgacgc atccccgttt
     9541 cgcgcacccc agagggcgcc tgcgatggcc ccgatggtat cgacgtcacc gccgagcgcg
     9601 gcaacgaact cgagcagttc aggaaaaggt cgatcgagaa atcgaagggc aacatagatg
     9661 gccgtgatgc aggactcggg cgcggcgatg ccgttgccga ggtgctgccg gacttgcggc
     9721 ggtgaaggac gctcgttatc caaccatgac ttggcgaccg ctaggcgttc acgaaatggg
     9781 tcttgttcgg cgatggctgc gagcgcatcg aggactcgcc ttgactcgtc gctgcggagc
     9841 agcaaggcgg tggctagcgc cacgagcacg gcgccttctt gaccaagcgg atgcgcgtga
     9901 gtgacctcgg ctgtcgcgcg ggctcgcgcc tgaacggcag caagatccga gcaagagaag
     9961 agccccacga tgggtgcgcg catggcgccg ccgtttccga aagagccatc gcggaaaaag
    10021 cgagtgttgg ccgatcgcca atcgacgccg cgacgaatga gtttcagaat acgagcggcg
    10081 cccgggccat atcctctgga ccagcgatag ccacgggcaa agcggcgcgc gatgtcatcc
    10141 tgatttaccg cgcggcttgc gacgagtgac tcggctaggt cgagactcat ctgagtgtcg
    10201 tcggtccagc ggagccgacc gtcgtaggtg gtgccaatca ggcgccacag ggcgcgttcg
    10261 agcgggccgc cctcgaacgg cgccccgaaa gcatctccga gcgcgagccc aaggacggca
    10321 cccacgaatg aggactccag ctcagccaat tgcacggagg ggcaacgcca acgagctcgc
    10381 ccctattccg acc
//
LOCUS       DASTKU010000255         5725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_942184, whole genome shotgun sequence.
ACCESSION   DASTKU010000255 DASTKU010000000
VERSION     DASTKU010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942184"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFQ61_09270"
     CDS             complement(<1..353)
                     /locus_tag="VFQ61_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954684.1"
                     /translation="MHPSARSGHPAYVKRRQLRGLVGGLLLVPVLLSSSSRASPAKRL
                     VVSASAAGAEGVALSRQIDRFARAHPGVEVSLSPVPESADERHQLYVQWLNAAAPEPD
                     VLQLDVIWLPEFAAAG"
     gene            672..2624
                     /gene="kaiC"
                     /locus_tag="VFQ61_09275"
     CDS             672..2624
                     /gene="kaiC"
                     /locus_tag="VFQ61_09275"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HET9954685.1"
                     /translation="MARSRRRSPSTSASNPASNSVAKIPVVQKSPTGIDGFDEITNGG
                     LPRGRPTLVCGGAGCGKTLFGMHFLVHGATRYNENGVFLAFEEREQDLIENVGSLGFD
                     LNDLVGRKLLALDHIQVDPSEIEENGEYDLEGLFLRLELAIDSVGAKRVVIDTLETVF
                     GGLKNQTILRSELRRLFQWLKDKGVSAIITAERGDGTLTRHGLEEYVSDCVVMLDHRV
                     TEQISTRRIRVVKYRGTTHGTNEYPFLIDKSGISVVPITSAGLAHRVSNERVSTGVPK
                     LDTMLSGGYYRGSTVLISGTAGSGKSSLCAHFADATCRAGERFLYISYEESAPQIERN
                     MRSIGLELAKWREQGLLEIMALRSTSQGIEGHLALIHQRIRELKPSALVVDPIGTLSG
                     AGTLQDSHLMSVRLIDLLKSEGITALLTNLTHGGEATESTAIAVSSIVDSWLLVKALE
                     ANGERNRAMYVLKSRGTAHSNQIREFVITAHGIDLVEPYVGPEGVLTGTARIVQEGRE
                     KEAARARVAEAQRLKRGLERKRLALEQQIAAMREAFQSEEDEIRTKLAAERESDAIAD
                     AGRARMGELRGSFRPGQAPVRAELEASELGGSDMELGADVAPGSQAVPGSRTAARSQG
                     APGSEAKLRKRQNGKLRHNKRATSRA"
     gene            2744..3184
                     /locus_tag="VFQ61_09280"
     CDS             2744..3184
                     /locus_tag="VFQ61_09280"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HET9954686.1"
                     /translation="MGRTIASGSAHQKASEEVKPRARSASSTSKTASNAKVKRKEVQP
                     ELAETSDLADSRDVWTLRLYVAGDSPKSRTALANLKRLCAAQRLTDFKIEVVDLKKRP
                     ELAKADQILAVPTLIRKIPEPMKRIIGDLSNADRALVALELDGV"
     gene            3310..4989
                     /locus_tag="VFQ61_09285"
     CDS             3310..4989
                     /locus_tag="VFQ61_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9954687.1"
                     /translation="MKSNTSKPRAPSKELERRLTEAESTLEAIQSGTVDAVVVHGPQG
                     PQVYSLEGPDQPFRTFVEGMQEGALTLSGDGTVLYANRFLAELLGKDAEQVMGRDFAA
                     FVAEEYKFTFAELRDTALKRGTKGNLRLIGASNTIPVQLTLSPLNGTGSPACCGVVFD
                     LREREQMEHANAAREAAEKASEARDRFLAVLSHELRSPLGTILGWAQVLGMRADFDEK
                     TRHALQSIERNARAQAKLIEELLDVSRFMAGKLHLETEVIDFAQIVQSAVTSARLGLD
                     KSVELASHSGEVDAYVLGDPTRLQQVVTNLLNNAIKFTDPGGHVQVDVTTEAGQVVLR
                     VSDTGIGISPEQIPRLFGLFQQVELNSRKSNEPYSQRRKTGLGLGLAVSKQLVEAHGG
                     KIVGHSEGIGHGATFEVRLPRVAAPPAIPLREEGQRGRLAGIRVLLVEDELDLLDLTR
                     HVLEVDGASVSAAQSAAAALALLRTETFDVLLTDIGLPEQDGFALIREVRARGHHREQ
                     LPAVALTGFASNEHARLAKAAGFQMHLAKPVDSRELVQAVQSLTAPPRADD"
     gene            complement(5005..>5725)
                     /locus_tag="VFQ61_09290"
     CDS             complement(5005..>5725)
                     /locus_tag="VFQ61_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9954688.1"
                     /translation="GQQALEWFEQNEYLCVLMDCQMPVMDGYEATRRIRQIERQRNRP
                     RTPVIALTAHALVGERERVLAAGMDEFLSKPFRPNALEKLLRACAKSAAASPLPVNPA
                     AQPGNRDAQSNKRHSKDPESADLDHAVHRSEKLIRLFLEKIPTQLRELELALEAQNAT
                     RVRALAHKIKGSALAIAAEPMALVAQSLQRRAEANDLGQSARLELRELNARHLRVSAQ
                     LAHELAVRAAQTPADRDPGQS"
BASE COUNT         1134 a         1719 c         1831 g         1041 t
ORIGIN      
        1 ccggccgcgg caaactctgg caaccaaatc acgtccagct gcagcacgtc gggttccgga
       61 gcagcggcgt tgagccactg cacgtagagc tggtggcgct cgtccgcgct ctccggaacc
      121 ggggacagcg acacctcgac acccggatga gcccgggcga atcggtcgat ttggcggctg
      181 agggcgacac cctccgcacc ggcagcgctg gccgacacca cgagacgttt cgccggactc
      241 gcccgtgagg aactgctcag caacaccggt acgagcagca acccgcccac caatccacgc
      301 aactggcggc gtttgacata cgcaggatga cccgaacggg ctgacggatg catggcccga
      361 gcctcgaaag gaagaaacct tatcgcggtc atggcagcca gtcatccgat cccttggcgg
      421 cggaggacca ccgccgacgc ggtgccgatt gggtggtgga ttacacgcac gcgctcccca
      481 ggcactcggt cggatggcag tgagcttcga tgctcgtgcg ctcgcaggac tacacgcggc
      541 tcgtcgaatc ttcgatatcc gccggtctga cagccgatcc atgactggtc atcctggaga
      601 cggcgcgcca tcgtcattgg cattctgaat tccgaagctg tgaccctccc accgtggtat
      661 gcccgcccga catggctcga tcgcgtcggc gctcgccttc cacttccgct tccaatccag
      721 catcgaattc tgttgcaaag atcccggtcg tacaaaagtc gcctaccggg atcgacggtt
      781 tcgacgagat caccaatggt ggtttaccgc gaggcaggcc cacgctcgtg tgtggtggag
      841 ctggctgcgg aaagacccta ttcgggatgc acttcttggt tcacggcgcc acccgctaca
      901 acgagaacgg ggttttcctc gcgttcgaag agcgtgagca agacctgatc gaaaacgtcg
      961 gatcgctcgg gttcgatttg aacgacctgg tcggccgcaa gctgctcgcg ctggaccaca
     1021 tccaggtaga tcccagcgaa atcgaggaga acggtgagta cgacttggag ggcctgttct
     1081 tgcgcctgga gttggcgatc gactcggtag gtgccaagcg tgtcgtcatc gacacgctcg
     1141 agaccgtgtt tggcggtctg aagaaccaaa ccatcctgcg ctccgagctg cgccgtctgt
     1201 ttcagtggct gaaggacaag ggggtttccg cgatcatcac ggccgagcgc ggagacggca
     1261 cccttacccg ccacggtctc gaggagtacg tctccgattg cgtggtcatg ctcgatcacc
     1321 gcgtgaccga gcagatatcc acgcgccgaa taagagtagt caagtatcgc ggcaccaccc
     1381 acggtaccaa cgaatatccg ttcttgatcg acaagagtgg tatcagcgtg gttccgatta
     1441 cgtcggcggg cttggcgcat cgcgtgtcca acgagcgtgt cagtaccggg gttccgaagc
     1501 tcgacacgat gctgagcggc ggttactacc gaggaagcac ggtactgatt tcgggcaccg
     1561 cgggctccgg caagagcagc ctctgcgcgc acttcgcaga cgcgacgtgt cgcgcgggcg
     1621 agcgattcct ctacatctcg tacgaggaat ccgcgccgca aatcgaacgc aacatgcggt
     1681 cgatcggcct agaattggct aaatggcgtg aacaaggcct gctcgagatc atggccctgc
     1741 gctccaccag tcaaggtatc gagggccacc tggcgctgat acaccagcgt attcgagagt
     1801 tgaagccgag cgccctggtg gtcgatccga tcggcacgct ctccggcgca gggaccttgc
     1861 aggactcgca cttgatgtcc gtgcgcttga ttgacctgct gaagagcgag ggcatcacgg
     1921 cgctgctcac caacctgacc catggtggag aggccaccga aagtacggcc atcgccgtgt
     1981 cgtcgatcgt cgacagctgg ctactcgtaa aggcgttgga ggcgaacggt gagcggaatc
     2041 gcgccatgta cgtcctcaag tctcggggca cggcgcactc caaccaaatt cgcgagttcg
     2101 tcatcacggc tcatggcatt gacctcgtcg agccttacgt gggacccgaa ggcgtgctga
     2161 ccggaacggc gcgcatcgtg caagaaggcc gagaaaaaga ggccgcccgt gcgcgggtgg
     2221 ccgaggcgca gcgcctgaaa cgcggactgg agcgcaaacg gctcgcgctg gagcaacaga
     2281 tcgcagccat gcgagaagca tttcagtcag aggaggacga gatccggacc aagctcgcgg
     2341 cggagcgcga gagtgacgcg atcgccgatg cgggaagggc tcgaatgggc gagctgcgcg
     2401 gcagctttcg gccgggccaa gcacccgttc gcgcagaact ggaagcctct gagttgggtg
     2461 ggtcagatat ggagctcgga gcggacgtcg cgcccggctc acaggcggtg cctggctcac
     2521 gaactgccgc acgttcacag ggagcaccag gatcggaagc aaagcttagg aagcggcaga
     2581 atggcaaact gcgacacaac aaacgtgcta cgtcacgtgc ttgaagcagg atcccgagat
     2641 ggccacgcga aagcctaaga aagctccttc ggcccggcca gcgaccggtg acggctctac
     2701 cgggcgcgcc gaatcccgag aacgggcgag tcgttcgaag tcaatgggcc gcaccatcgc
     2761 ttctggttca gcgcatcaaa aggcttcgga agaggtcaaa ccccgggctc gcagtgcatc
     2821 gagcacgtca aaaactgcga gtaacgccaa ggtcaagcga aaggaagtgc agccggagct
     2881 ggccgagacg agcgacctcg cggattcccg agacgtttgg acgttacggt tgtatgtcgc
     2941 gggtgactca cccaagtctc ggactgcact agccaacttg aaacggttgt gcgcggcgca
     3001 acgcctgacc gatttcaaga tcgaggtcgt ggatctcaag aagcgccccg aactcgccaa
     3061 ggcagatcaa atcttggctg tgccaacgct gatccggaaa atcccagagc cgatgaagcg
     3121 tatcatcggc gatctctcca acgccgaccg agcactcgtc gcgctcgagc tcgacggtgt
     3181 ttagtgtgtc gcgctcgagc tcgacggtgt ttagtgtgtc gcgctcgagc tcgacggtgt
     3241 ctagagtgtg tcgcgctcga gctcgacggt gtctagagtg tggctgacgg tgcgccgcac
     3301 cggtgagtta tgaagagcaa cacctctaag ccgcgtgccc cctcgaaaga gctcgagcgg
     3361 aggctcaccg aagccgaaag cacgctggag gcgatccaga gcggaactgt cgacgcggtc
     3421 gtcgtgcacg gcccgcaagg tccgcaggtg tactcgctgg aaggtcctga tcagcctttt
     3481 cgaactttcg tggaggggat gcaagagggg gccctgactt tgagcggaga cggcacggtt
     3541 ctttacgcga atcgattcct cgcggagcta ctcggcaaag atgccgagca ggtgatgggg
     3601 cgggacttcg ctgccttcgt cgccgaagag tacaagttca ccttcgcgga gctccgcgat
     3661 acggcgctca agcgtgggac caaaggcaat ttgcgtctga ttggcgcttc caacaccatc
     3721 ccggtgcagt tgacgctcag ccccttgaac gggacgggat cgccagcctg ttgcggcgtc
     3781 gtgttcgatc tgcgtgaacg agagcagatg gagcatgcca acgcggctcg ggaggccgct
     3841 gagaaggcca gtgaagcgcg cgaccgcttc ttggccgtgt tgagccacga gttgcgctcg
     3901 ccgcttggga ccatcctggg ctgggcacaa gtcttgggta tgcgcgcgga cttcgacgag
     3961 aagacgcggc acgcgctgca aagcatcgaa cgcaatgccc gcgcgcaagc caagctcatc
     4021 gaggagctgc tggacgtctc gcgcttcatg gcgggtaagc tgcatctgga gaccgaggtc
     4081 atcgactttg cgcagatcgt gcagtcggca gtaaccagcg cgcgcctggg gctcgacaag
     4141 tcggtggagc tcgcgtctca ctctggagaa gtagacgcat acgtgctggg ggaccccacg
     4201 cgcttgcagc aggtggtgac caacctgctc aataacgcga tcaagttcac cgatccgggt
     4261 gggcacgtgc aagtcgacgt caccaccgaa gcggggcagg tcgtactccg cgtgtcagac
     4321 accggtattg gcatttctcc cgagcagatt ccccgtctat ttggcctctt tcagcaggtc
     4381 gagctgaact cgcgcaagtc gaacgaacct tactctcaac gtcgcaagac gggtttgggc
     4441 ttggggctcg cggtgagtaa gcagttagtc gaagcccacg gaggaaagat cgtcgggcat
     4501 agtgaaggga tcggccatgg cgctacgttc gaggtgcgtc tgccacgagt agcggcgccc
     4561 cccgccattc ccctgcgcga ggaaggtcag cgcgggcgcc tcgcggggat cagagtgctg
     4621 ctcgtcgaag acgagctaga cctcctggac ctaacgcgtc acgtattaga agtggatggc
     4681 gcgtcggtga gcgctgctca aagtgcggcc gccgctctag cgctactacg caccgaaacc
     4741 ttcgatgtgc tgctcacgga cattggtttg cccgagcagg acggcttcgc cttgatacgc
     4801 gaggtgaggg cgcgcggtca tcatcgtgag cagctgccgg cggtcgcgct cacgggcttc
     4861 gcaagcaatg aacacgcccg cttggccaaa gcggcaggct ttcaaatgca tctggccaag
     4921 cccgtggact cacgcgagct cgtgcaggcg gtccagagcc tgacggcccc gcctcgcgct
     4981 gacgattgat ggcgattccg gcgctcacga ttggccggga tcgcgatcgg cgggggtctg
     5041 agcggcgcgc acggcgagct catgagcgag ttgggcgctg actcgcaggt gcctggcatt
     5101 cagttcccgc agctccaatc gagcactctg ccccagatca ttggcttccg cgcggcgttg
     5161 taaggactgg gcgacgagtg ccatcggttc tgccgcgatt gccagcgcac tgccctttat
     5221 cttatgcgca agcgcgcgaa ctcgcgtggc attctgcgct tcgagtgcga gctccagctc
     5281 acgcaactgg gttggaatct tctccaggaa cagccgaatc agcttctccg atcgatggac
     5341 tgcatggtcg agatccgcgg actcgggatc tttggagtgg cgtttgttgg attgggcgtc
     5401 tctattcccc ggttgcgccg ctgggttcac tggcagaggg ctcgcggcgg ctgattttgc
     5461 acaagctctg agcaacttct cgagtgcgtt gggtctaaac ggctttgaca gaaactcatc
     5521 catgcccgcc gccaagacgc gctcgcgttc accaaccaaa gcatgagccg tcagtgcgat
     5581 gactggggtt cggggccggt tgcgttgtcg ctcgatctgt cggatgcgac gggtcgcctc
     5641 gtagccatcc atcacgggca tttggcaatc catcagaacg cacagatatt cattctgctc
     5701 gaaccactcc agcgcttgtt gcccg
//
LOCUS       DASTKU010000256         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_50939,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000256 DASTKU010000000
VERSION     DASTKU010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50939"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2258)
                     /locus_tag="VFQ61_09295"
     CDS             complement(<1..>2258)
                     /locus_tag="VFQ61_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9954689.1"
                     /translation="ISGPLLLVLLEAVRWLELRWVRFSRPLACLPVFVTQAWILLRLR
                     GYGARTSAARRRAFESSVGAAVALAGLAVSGLEIGHAVDRLTILIALDRSRSIELVPG
                     AETRIERELAVAELGMRPSDRIGTVAFAADARIEDPVRPKSTLRGAQRVDIARDGTDI
                     SQAIRQALSSIPPDSAARIAILSDGVATRGDTLEAALAASALGVPIDVVALDQGSPKD
                     VRVVSVRVPPHGAKGETLDVRIVTHASQQTRVTIRLLRDGDLIREGPATLAQGEDVVT
                     LREIAPEPGLHRYDVELSVEDRSVDRAPEDNSGSAFMRVRGESTALVLDSDPAFGAAT
                     AQALRNAAFLVDTAGPTGIPADLAGLARYDLIVLGDISAPEFSPQQLLAMQSYVRDLG
                     GGLLLLGGDKTMGPGGFARTPIEEVSPVSFDLKQERRRASLAEVIAIDYSGSMAATVD
                     GRTKLELANEAAARSSELLGEGDRLGVMHVDTAVSWTLPLANVTDTAEIGNRIRRVGP
                     GGGGIFIDLSLSAAYQALARESVQLKHLLLFSDGGDAEERARAPGLVERAREKGITTS
                     VVALGNGSDVPALSRMAELGGGRFYLIEDAARLPAVFAQETVLATRSAIHEVAFVPHP
                     LSPSRVLSGVSLQQAPPLKGYVVTLPKPRAQVLLAGPEADPILATWSVGVGRAGAFTS
                     DYKDRWGSEWLGWPAAARLFAQLGRDLGRRLDDPKVRFEASTQNGELTLQASVVDEQG
                     RHASFRQLTARV"
BASE COUNT          398 a          771 c          707 g          382 t
ORIGIN      
        1 aactcgcgcc gtcaactgcc gaaagctcgc gtgacggcct tgctcgtcta ccacgctggc
       61 ttgcaacgtg agctccccgt tttgggtgct ggcttcgaaa cgaaccttgg gatcatccag
      121 gcgacgcccg agatcgcgtc cgagttgtgc gaacaggcgc gctgccgccg gccagcccaa
      181 ccattccgaa ccccagcgat ctttatagtc actggtgaac gcgccggctc gacccacgcc
      241 caccgaccaa gtcgcgagaa tcggatctgc ctccggtccc gcgagcagaa cttgcgcacg
      301 cggcttcggc aaggtcacca catatccctt caagggggga gcttgttgaa gcgagacgcc
      361 ggacagcacg cggctcgggc tgagcgggtg ggggacgaac gcaacttcat ggatagcgct
      421 acgcgtggcg agcacggttt cttgcgcgaa gaccgccggt aaccgcgccg cgtcctcgat
      481 caaatagaaa cgcccccctc ctaactccgc catccgagac aacgccggaa catcagagcc
      541 gttgccgagt gccacgacac tcgtggtgat gcccttttcg cgtgcgcgct cgaccagacc
      601 tggtgcgcgg gcccgctcct cggcgtcccc gccatctgaa aacagcagca ggtgcttgag
      661 ctgtacgctc tctctcgcga gcgcctggta ggccgcactc agtgaaagat cgatgaaaat
      721 tccgccgcca ccgggcccga cacgacggat acgattgcca atctccgccg tgtccgtgac
      781 gttggcgagc ggcaaggtcc agctgaccgc cgtgtcgacg tgcatgacgc cgaggcgatc
      841 tccttcgccc agcagttcgg aagagcgtgc cgctgcctcg ttggccagct cgagtttggt
      901 ccgcccatcc acggtggcgg ccatagaacc ggagtaatcg atcgcgatga cctcggccaa
      961 actcgcccgg cgccgctctt gcttgagatc gaacgatacc gggctcacct cttctatagg
     1021 ggtgcgggcg aacccgccgg gccccatggt cttgtctccc ccgagcaaca gcagtccccc
     1081 gccgaggtcc cgaacgtagc tctgcatcgc gagcagttgc tgtgggctga actctggagc
     1141 gctgatatcg ccgagcacga ttaaatcgta tcgggccaag ccggccagat ctgcagggat
     1201 gccggttggg cccgccgtgt cgaccaggaa cgccgcattg cgcagcgctt gagccgttgc
     1261 ggcgccgaag gccggatccg agtcgagtac gagcgccgtg gactcaccgc gcactctcat
     1321 gaacgcggag ccggaattgt cttctggtgc gcgatcgacg ctgcgatcct cgacgctcag
     1381 ttccacgtca tagcgatgga gtccgggctc cggggcgatt tcacgcagcg tgaccacgtc
     1441 ctcgccttgc gccagagtcg caggtccctc tcggatcagg tccccgtctc gtagcaggcg
     1501 aatcgtcacc cgggtttgct ggctggcgtg cgtaacgatg cggacatcga gggtttcacc
     1561 ctttgcaccg tggggaggca ctcggacgct cacgacccgc acgtctttgg gggaaccctg
     1621 gtcgagagcg accacgtcga tggggactcc caacgcgctc gccgcgagcg cggcctccag
     1681 cgtgtcaccc cgcgtggcta cgccatcact cagaatcgcg atgcgagccg ccgagtccgg
     1741 cgggatgctc gatagagcct ggcgaatggc ctgcgagata tcggtgccgt ctcgtgcgat
     1801 gtccacccgt tgtgctccac gcaatgtgga tttcgggcgc accgggtcct cgatgcgagc
     1861 atcggcggcg aacgccaccg tcccgatgcg gtcggagggg cgcataccga gttctgccac
     1921 ggcgagctcg cgctcgatgc gcgtctccgc tcccggcacg agctcgatac tgcggctacg
     1981 gtccagcgcg atcaatatag tcaggcgatc gacggcgtgt ccgatctcga gcccgctgac
     2041 cgcgagcccc gccaacgcga cggcggctcc cacgctcgat tcgaaggctc ttcggcgcgc
     2101 cgcgctcgtg cgcgctccat aacccctgag ccgcaacagg atccaagcct gggtcacgaa
     2161 gacgggcagg caggcgaggg gtcggctgaa gcgcacccac cgcagttcca gccagcgcac
     2221 ggcttccaac agaaccaata gcagggggcc gctgatcg
//
LOCUS       DASTKU010000257         2847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1835905, whole genome shotgun sequence.
ACCESSION   DASTKU010000257 DASTKU010000000
VERSION     DASTKU010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835905"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFQ61_09300"
     CDS             complement(<1..277)
                     /locus_tag="VFQ61_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954690.1"
                     /translation="MRQLRVLPFVASLLSCGGSGADEGLAQGGAAPLAGTSAMSGAAS
                     QMGAMGGASNVGQGGAAGASATSGGALNRAGAGTATATGGSSMSPGGS"
     gene            500..1441
                     /locus_tag="VFQ61_09305"
     CDS             500..1441
                     /locus_tag="VFQ61_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954691.1"
                     /translation="MVGSSAVYATQLTAQMAANRSALPYYAAFGTHRERLSSRILAAE
                     RSLTQNADQLSALKSERATAKEPRRTLCVLGAGNGYDLDFPLLHTRFAEMHLVDLDAD
                     ALRRCHDRQEPRIQAALGLHAGLDLSGMLDRLERWRRMQVTPQELMNHAEGTARALRV
                     ALGRSFDVVVSACMLSQMQLTLLEALGAEHRLFEAARMTLTLTHLRALGALLAPGGVA
                     VFATDVSAEGILPSGALAPFETASELDRGELVRDLARRGHVFHFADPERVLELIREDP
                     VLHRNFSSSDVTDAWVWQNGSAGKFLVYSLELRNRVL"
     gene            complement(1511..>2847)
                     /locus_tag="VFQ61_09310"
     CDS             complement(1511..>2847)
                     /locus_tag="VFQ61_09310"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9954692.1"
                     /translation="YGFQDRLSCPGGGGHEAGTPFTTTCANAPGTRANGGEGDDGVAG
                     GPSFDQIFLKHVPGLKKQGAGYINTLADARVDSLETSTQCLSYGEGIPGTADYRTRSI
                     KAANPSGSNITEFTPNLPELSPANAYATLFSSFIPGGATGTNNDAAIKALKARKSVLD
                     HALREIATLKSLAPAAESSKIDAHAQAIRDVETQLSTQIAEGGLTTGPSCTKPTAPDA
                     SLKGKTGSKFDYGNEGTSTSDEETHEKIGKAHAAVILAAFQCDIVRVATFQWSPGTNH
                     VSFKGKYPAQPERSFMHHPMSHRIGAQSFFNGPPLTGTDADASLYQFLVAVSAWYSQK
                     MADILGTFKNAKDSFGNSILDYTVVPYLTEVGDPSHSRSPKASLIFGGKGLGMKGGQF
                     LNFDGNLRPQVDVWLTVAQAMLQTDDPLKALPTTEKFNRTNAKPIAGLWAPP"
BASE COUNT          486 a          884 c          891 g          586 t
ORIGIN      
        1 gtgagcctcc tggagacatg ctcgagccgc ctgtagccgt ggctgtacct gcacccgctc
       61 ggttgagagc cccgccgctc gtcgcgctcg cgcctgccgc gccgccctgg cccacgttgc
      121 tcgctccacc cattgcgccc atctggctag ctgcgccgct catcgccgaa gtaccggcca
      181 acggcgctgc tccgccctgc gccagaccct catccgcccc gctcccacca caagagagga
      241 gtgaagccac gaagggcaga actcggagtt gtctcatctt caactttcct gttcaggtga
      301 atgagccaga tgttctggtt cgcgagcgcg taaccagttg tgagtgacgt catggccgca
      361 ggctgggtca cctaaatgca cccggtgttc gattttgcgt gttcactttg gaatcacgtc
      421 acaaatggga gacatggctc acgctcccaa ggatgcctct ccacacgcta gctcctcaga
      481 gactgcctta gagcacgcct tggttgggag ctccgctgtc tacgccacgc agctcaccgc
      541 ccagatggcc gcgaaccgta gcgcgctgcc ctattatgcg gcgttcggca ctcatcgcga
      601 acggctctcg agtcgcatct tggccgcgga gcgcagtcta acgcagaatg cagatcagct
      661 ttcggcgctg aaaagtgagc gcgcgacggc aaaggagccg cggcgcaccc tgtgtgtgct
      721 cggcgcgggg aacggctacg acttggactt tccgctgcta cacacgcgct ttgcggaaat
      781 gcacttggtg gacttggatg cggacgcgct gcgccgctgt cacgaccggc aggagcctcg
      841 aatccaagct gccctcggcc tgcacgctgg cctcgatctg tctggcatgt tggatcggct
      901 cgagcgctgg cgtaggatgc aagtcactcc gcaagagctc atgaatcacg cggagggcac
      961 cgcccgggcg ctgcgtgtgg cgttgggcag aagcttcgac gtggtggtgt cggcttgcat
     1021 gttgagccaa atgcagctca cgttgctcga ggcgctgggc gccgaacatc gcctattcga
     1081 agcggcgcgt atgaccctga cgttgaccca cctgagagcg ctcggggcgc tcctggctcc
     1141 aggcggagtg gccgtgtttg cgaccgatgt gtcggccgaa ggcattctgc cgtcgggcgc
     1201 attagcgccg ttcgaaacgg cttcagagct cgatcgagga gagctcgtgc gggatctggc
     1261 tcgccgaggg cacgtgtttc acttcgccga tcccgaacgc gtgctcgagc tgattcgaga
     1321 agacccagtg ctccatcgaa acttcagcag ctcggatgtg actgacgcct gggtttggca
     1381 aaatggcagc gccggcaagt tcctggttta tagcttggag ctgcgaaatc gggttctctg
     1441 aatccacggc gattacggcg gagccccaag gcccgctgtt gttatggcgg agccccaagg
     1501 cccgctgttg ttatggcgga gcccaaaggc ccgctatggg cttcgcattc gtgcggttga
     1561 acttctcggt ggtggggagt gctttgagtg ggtcgtcagt ttggagcatg gcttgcgcga
     1621 ccgttagcca gacgtcgact tgggggcgca aattcccgtc gaaattcaga aattgcccac
     1681 ccttcatgcc caagcctttg ccgccgaaga tcagcgacgc tttgggagat cgcgaatggg
     1741 aggggtcgcc cacctcggtc agatacggca cgaccgtata atccaagatg ctgtttccaa
     1801 aagagtcttt ggcattcttg aacgtcccca gaatgtccgc catcttttgg ctgtaccagg
     1861 cactcaccgc caccaggaat tggtacagtg aggcgtccgc gtccgttccc gtgagcggcg
     1921 ggccgttgaa gaacgattgc gcgccgatgc ggtgactcat cgggtgatgc atgaagctcc
     1981 gctcgggctg cgcgggatac ttgcctttga aggagacgtg gttcgtgccg ggggaccact
     2041 gaaaagtggc gacccgcacg atgtcgcact gaaacgcggc caaaatcacc gccgcgtgcg
     2101 ccttaccgat cttctcgtgc gtctcctcgt cggaggtact ggtgccttcg ttgccgtagt
     2161 cgaacttcga gcccgtcttg cctttgaggc tcgcgtcggg cgccgtcggc ttggtgcagc
     2221 tcgggccggt ggtgagcccg ccctctgcga tttgagtact cagctgcgtc tccacatcgc
     2281 ggatcgcctg tgcatgcgcg tcgatcttcg agctctcggc ggctggggcc aggctcttca
     2341 gcgtcgcgat ctcgcgtagc gcgtgatcga gcacgctctt gcgcgccttg agcgctttga
     2401 ttgcggcatc gttgttcgtg cccgtcgcgc cacccgggat gaagctacta aacagagtgg
     2461 cataagcgtt ggccgggctc agctcgggca ggttgggcgt aaactccgtg atgttcgagc
     2521 cagacggatt ggcggccttg atcgaccgcg tccgatagtc ggcagtaccg gggatcccct
     2581 cgccgtacga cagacactgc gtcgaagtct ccagcgaatc cacgcgcgca tccgccaatg
     2641 tgttgatgta tccggcccct tgcttcttga gccccggcac atgcttcaag aagatttgat
     2701 cgaacgaggg gccaccggcc acaccatcgt ctccttcacc gccgttggcg cgcgtaccgg
     2761 gagcgttggc gcaggtcgtg gtgaacggag tgcccgcctc gtgtccaccg cctccgggac
     2821 acgaaagccg atcctggaat ccataca
//
LOCUS       DASTKU010000258         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_497856, whole genome shotgun sequence.
ACCESSION   DASTKU010000258 DASTKU010000000
VERSION     DASTKU010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497856"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..847)
                     /locus_tag="VFQ61_09315"
     CDS             complement(251..847)
                     /locus_tag="VFQ61_09315"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9954693.1"
                     /translation="MFKRAFGSMSLVLALMALPASALASPAQDFVKAKQTELTELVKK
                     SKSAEDKKLEGAFDAVLDYETLAHDTLREFWEERTPSERAEFQEALKKLVRNAYRKNL
                     KRIGDFEVEYKGESNADSGQLVRTVAKNRSNAREEPVSIDYIVREVGGKWKITDIVTE
                     GSSMVGNYRNQFRRIIKKQGFPELIKRMKTKLDKTDAE"
     gene            complement(1051..2379)
                     /locus_tag="VFQ61_09320"
     CDS             complement(1051..2379)
                     /locus_tag="VFQ61_09320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9954694.1"
                     /translation="MNKKLSFGWHGVGPGPLPRRLAWLTLARLLFLTLALALVSAFYL
                     HEQANIGSLTPRFALATLAVSFALAAVYASILRRGRGLELLADVQLVLDQVNWTVVAY
                     LTGGATSGATSFYGLSCLVGASLTGMRGAAVAAISGAVCYGSLALLLQKGWLKPPPDQ
                     PAALYALSADEFWFYLLVNTLVLVVVSLLSGTLVERLRRTGGALVAATERADRAERMA
                     ALGRLAAGLAHEIRNPLGSIAGSIQLLRTARGLSQEDRRLCEIIQREAARLNDLVTDM
                     VDLSRPRLPMLADVDVGAIAKEVVELASGSGRAVSDVAIQYLGLSSAKVRADGAQLRQ
                     LIWNLVRNAVQASEPGAVVLVQVELDQRGHVELRVTDRGKGIDESARERLFDAFFTTR
                     SQGTGVGLAVVKRIADEHHFPIRVESQEGQGASFVVVLAPEPSASQPVLA"
     gene            complement(2376..>2963)
                     /locus_tag="VFQ61_09325"
     CDS             complement(2376..>2963)
                     /locus_tag="VFQ61_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9954695.1"
                     /translation="LVMDFSHFFVSALPFLIVGGIGAAVGISYLYRTPRGKRFAHKTM
                     LKLPVMGPVLRKIAVARFTRTLGTLLQSGVPILDALEICAKTSGNVVIESGIMSVRQA
                     ISEGRNMAEPLTQTGIFPDMVVQMIAVGEQTGALDQMLNKIADFYEEETDVAVAAMTS
                     ALEPILMVGVGGRVGVVLMAMYLPIFSLAGNIKAD"
BASE COUNT          594 a          978 c          851 g          540 t
ORIGIN      
        1 ttcgaccatc agccagccca tcggggactg ccgccgactc tttcggatcc aacgccgact
       61 ccgccacgtt ctttcaccgt cttcgttacg gcgcccgtag cgtaccagct gcggctatgt
      121 gacggccaag tccctggcaa gactttgttg gctcgtcgct cgggtggcag ctcgtgcggg
      181 cgcgcggtgc ctcggaccgg cggctcggag ctcgacgggc cgccggtgag gcagccgctg
      241 agggcgtcga ttactcggcg tccgtcttat cgagcttggt cttcatgcgc ttgatcaact
      301 cgggaaagcc ctgcttcttg atgatgcggc gaaactggtt gcggtagtta ccgaccatac
      361 tggacccttc ggtaacgatg tcggtgatct tccacttacc gccgacttcc cgcacgatgt
      421 aatcgatgct cacgggctct tcccgagcgt tggagcgatt cttcgcgacc gtccgcacca
      481 gctgcccgct gtccgcattc gattcgccct tgtactccac ctcgaaatcc ccgatgcgct
      541 tcaggttctt tcgataagcg ttgcgaacca gcttcttcaa agcctcttgg aactccgcgc
      601 gctcgctcgg cgtccgctcc tcccaaaatt cacgcagagt gtcgtgagcg agagtctcgt
      661 agtcgagcac ggcatcgaag gccccctcga gcttcttgtc ctctgcgctc ttgctcttct
      721 tgaccagctc cgtcagctcg gtctgcttgg ccttgacgaa atcctgggcg ggtgaggcca
      781 aggcgctggc gggcagagcc atgagagcca aaaccagaga catagatccg aaagcacgct
      841 tgaacattgt tggctccgtg aatttaaggt tcgacattcg gtacgcaagc ccggtgccaa
      901 gccttccctc gatattttgc agaatcccac ggtgctgcgc gtcaacccgg cgctacaggc
      961 gtatccgatt cgcaacagta gatcgatgcg agccggcttt acgcagcgtg gcccccagag
     1021 cttcccctcc cgcaagctct gacccgatgc tcaggcgagc acgggctgag acgccgaagg
     1081 ttccggcgcg agcaccacca cgaacgatgc tccctgcccc tcctggcttt cgacgcgaat
     1141 cgggaagtgg tgctcatcgg cgatgcgttt caccacggcg agtcccaccc cagtgccttg
     1201 ggatcgagtg gtgaagaatg cgtcgaacag gcgctctcgt gcgctctcgt cgatcccctt
     1261 acctcgatca gtgacgcgaa gctccacatg ccctcgctga tccagttcca cttgaaccag
     1321 caccacggcc ccaggctccg aagcctgaac ggcgttgcgc acgagattcc aaatcagctg
     1381 cctcagctgt gcgccatcgg cgcgcacctt tgccgaagat agccctagat actgaatcgc
     1441 gacgtctgaa acggcgcgcc ccgatcctga agcgagctct acgacttctt tggcaatcgc
     1501 tccaacatcc acatccgcga gcattggcag gcgcggacgg gacaagtcca ccatgtccgt
     1561 caccagatca ttgagacgcg ccgcctcacg ctgaatgatt tcacacaagc gacggtcctc
     1621 ctgcgacagc ccgcgtgccg tgcgcaagag ctggatagag cctgcgatag agccgagagg
     1681 gttacgaatc tcatgcgcaa gccccgccgc gaggcgtccc agggccgcca tgcgctcggc
     1741 tcgatcggcc cgctccgtcg ccgccaccaa tgctccgccc gtccgccgca gccgctccac
     1801 caaggttccc gagagcagag acacgacgac gagcacgagc gtgttgacca gtagatagaa
     1861 ccagaattcg tctgccgaga gtgcgtacag cgccgccggc tgatcgggcg gcggcttgag
     1921 ccagcccttt tggagcaaga gagccaagct gccgtaacag accgcgccag aaatcgcggc
     1981 caccgccgcg ccgcgcattc cggtcaagct cgcgccgacc aagcagctca aaccgtaaaa
     2041 ggaggtcgca ccgctcgtag ccccgccggt caagtaggcc accacggtcc agttcacctg
     2101 gtccagaacg agctgcacgt ccgccagcaa ttccaagccg cgcccgcggc gcagaatgga
     2161 ggcataaacg gccgccaacg cgaacgacac ggctaacgtt gcgagcgcaa agcgaggcgt
     2221 gagggagcca atattggctt gctcatgcaa atagaaggcg ctcaccagcg ccagcgccaa
     2281 cgtcaaaaac agtaggcgag cgagcgtgag ccaagccaga cgtcgcggaa gtgggcctgg
     2341 gccgacgccg tgccagccga atgacagctt cttgttcaat ccgctttgat gttgccagcc
     2401 aaggagaaga ttggaaggta catcgccatc aacacgacgc ccaccctgcc accaacacca
     2461 accatcaaga tcggttcgag cgccgaggtc attgcggcaa cggcaacgtc cgtctcttct
     2521 tcgtagaaat ccgcgatctt gttgagcatc tgatcgaggg cgccggtttg ttcacccacc
     2581 gcgatcattt gcacgaccat gtctgggaag atgccggtct gggtcaaggg ttcggccatg
     2641 ttgcgacctt cgctgatggc ttgccgcacc gacatgatgc cggactcgat cacgacgttg
     2701 cccgaagtct tcgcgcagat ctcgagagcg tccaagatgg gaacgccgga ctgaagcagc
     2761 gtgcccagcg tgcgagtgaa gcgcgcgacg gcgatcttgc gcagcaccgg tcccatcacc
     2821 ggcagcttca gcatggtttt gtgcgcgaag cgcttgccgc gcggagttcg gtacaaatac
     2881 gagatgccga ctgcggcccc aatcccgccc acgatcaaga acggcagcgc cgaaacgaag
     2941 aagtgcgaga agtccatgac gag
//
LOCUS       DASTKU010000259         7987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1166369, whole genome shotgun sequence.
ACCESSION   DASTKU010000259 DASTKU010000000
VERSION     DASTKU010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166369"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2232)
                     /locus_tag="VFQ61_09330"
     CDS             complement(<1..2232)
                     /locus_tag="VFQ61_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954696.1"
                     /translation="MGRRLAEPEAGFEVVRVAAERGLAETLEHHLGRQAGLVGELLVY
                     FSGYTVLSQERGPALLLDGERLGTFSLRRLRGLFARFSHRACLIVDAAALVDSGQSLD
                     AVVTGIGEALTEGVPSISALVAVRDSSVPDSFGGSAFTGLMLTALDWLAVSREAGEAV
                     DIERLYEALRAEPALFQEIPATGLFGGMEPRLSILQPGVPPQRAARAEGPRKVRAEAS
                     APLPSFADLPNLDADLAALLGFEAQPEPVREEPTPALARGATPPGAFEQSTLEPRSSG
                     QPGEQPGVLEQPGAVEQQGGLERSAFQPAPGLATPGGWSGPEQAILPFAQTEHAATPA
                     AHAFSAAMPVAADAVPTEAPPHESVSPRDAARARAAELLSYYANALLRAVDSDELSRI
                     SAELAVLVRERQVSPARAALAWEACLARFPRDLDLREQLVEACTQASAPARALLHCQA
                     AAQIAPLRAATYRRAQALFESVGAADGAWQAASVLEALGDADINESLLAHQHKPDGLL
                     MPRGTVSDAEFFGALCPTSATVLLLPLLSVLARADAVADAALGIGFLKHKKVYQEPDP
                     SYLHDAERSTAMFAKTLNWAARLFGQKVPSLYVFPELPSLTAPGTLREPSVYCARALG
                     SGLSLTQLAFLWGRELPRQRPEFRMLAFFKRPDELAALIAASLTLAGVPGYDVRALSA
                     DAKRLYTALRRELSRESLESLRDAGSDIAPEELEQRAGMALAEFELLSVRAGLLACGD
                     VVTA"
     gene            complement(2410..3750)
                     /locus_tag="VFQ61_09335"
     CDS             complement(2410..3750)
                     /locus_tag="VFQ61_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9954697.1"
                     /translation="MPEDVPVLARARALAEHAHRGQLRKAGGRPYFEHLSGVEKILLD
                     HGYSDPLILAVGYLHDVLEDQPASADRLRREMPPEVVAAVELLTESKTDVSGRKRPKA
                     ERFAEYAARLAAAGNEAELALPVSCADKIHNWSSLVETEAAGESLLSRLATRPGDYAE
                     QHARLRRVFHGRVNARLLRRFDQAAEDLQRTISNWLPGRAAMLAAEANLGRFDAHGEP
                     AIFSVLRVASKLDKPLERLTWLLQDALRLRSLEELSAEGFPEEVVRALPHVAHREEES
                     EEEWLRRLSEQRLAARIVLLHEKFGSEAHLRLVKRLEAELAKRSLWVVLDRSSVDCVR
                     RMSLLPVVRGDHVTLAHRVDPHRFAPEWVPGGHRPGERVALRAVSQVCDERLQALALE
                     MAGSTHRPFDGGPLHLTVSRVRGARSREANSLLERAPRQRVDLELSGQIVWVEA"
     gene            complement(3785..4828)
                     /locus_tag="VFQ61_09340"
     CDS             complement(3785..4828)
                     /locus_tag="VFQ61_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954698.1"
                     /translation="MKARWLCVGLQFWVVTASMSAHAAEVAVSEEARAHFSAGVNLLQ
                     DPDGARYEEAYREFRAAYAASPSWKILGNLGITAMKLERDGEAIEAFARYLSEGGKQL
                     DPEERAQTERDLQTLKTGVVRIAFNASRTGARLVDERSLASGSSVVNRYGPIDSTLEI
                     GVRAGHHRITASLAGFKDIVLELDAEPQQRLVYPLEFQAADAVAIAPPPMSSSNARSD
                     VLVPGSSLESDRRPLLRTLSYAAFGVGVVGIGAGTWLALSAKSRFDDANEMCPEFPCY
                     LTQAQQDRRNELGDSGQSRKTLSLVGFVVGGVGLATGATLLVLGTRQNEKTASRPPEV
                     WVGPSSLGMRGQF"
     gene            5020..6429
                     /locus_tag="VFQ61_09345"
     CDS             5020..6429
                     /locus_tag="VFQ61_09345"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9954699.1"
                     /translation="MPVRSPLPTWFRACSVGCLLTAALATSAFGAAPPVPAAPAEANL
                     QIPFEKYLLNNGLEVILHQDRSLPLVAVNLWYHVGPANEPPGRSGFAHLFEHLMFKGS
                     RHVGRDFDKLLEAAGATNVNGTTFWDRTNYFETVPREHLELALWIESDRMGYMLDGLD
                     QATLDAERDVVKNERRQSFENSPYGKSSLVLLNTLFPQGTAYHGAVIGSMEDLSRASL
                     SDVREFFHTYYAPGNATLTLAGDFDVAQAKAWITKYFSTLAARPRPASTAQPISAVTR
                     SEHLTVKEPVELTRVSFGHVTPPAYSSDDPVLEVTAAILAGGKATRLYRALVLEGKLA
                     SEVDAAVDSNQLASNFTLTATVASGKSATQVERAMQTVLDQLAERGPTAAELDRARRR
                     ILLNVLGNLELLNGSGGEAGRAGILQRFNHYLKDPGYLPKWVEQIRAVKGEDVSRVIR
                     AHLSSEHRIVVTTLPEVTP"
     gene            6426..7943
                     /locus_tag="VFQ61_09350"
     CDS             6426..7943
                     /locus_tag="VFQ61_09350"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9954700.1"
                     /translation="MNALATRQSAWLVLVGSLLFQAACSPARATGPSAKPAPTAVVPV
                     SAGNPDPEPFRARKPQPGSPGEFTYPTPKVVKLPSGLSVYLVQRPARVVAIEFNVRHG
                     SASDPEGKSGAAALCARMLTEATRRKSALALSEAVEALGTNLQASASRDSSNVALSAL
                     PGDVEAALALLAEVITEPAFAPADFERVRAEWLDGLRAERQDPQRLAILLANRWLLGP
                     RHGAPSGGRLGDVQKLTVGDLRAFHRQAYTPDNSALMLVGNFDENEVMKRVQALFGNL
                     RGKDAIAPPPPVPESPLTQTQIRVLDRKDAVQSAIAVIQPFPRRSEAGFEARQILGRL
                     LGGLFTSRLNTRLREEHAYTYGAAATPWAAKHSGALLVYTSVRTDVTAPALTDILGEL
                     DRTRDPKLGAPVGEEEMQRAKADLIFSLGASLEHPSRIADRIGASFIDGQAPEYDANY
                     PALIRALPLPAVQMSGAALSTASRVVIVGDKVQITESLSKLGMPVESVPEGLLEN"
BASE COUNT         1445 a         2733 c         2455 g         1354 t
ORIGIN      
        1 cgctgtcacc acgtccccgc acgccagaag ccctgcgcga acactcagca actcgaactc
       61 ggcgagggcc attcccgctc gttgttctag ctcctccggc gcgatgtccg agcctgcgtc
      121 gcgcaagctc tccaggctct cgcgcgacag ctcccgccga agcgccgtat acagccgctt
      181 ggcatccgcc gagagagcgc gcacgtcgta gcccggtacg cccgccaacg tcagcgaagc
      241 ggcgatcagc gcggcaagct cgtccggccg cttgaagaac gccagcatgc gaaactccgg
      301 acgttggcgg ggcagctcgc ggccccaaag aaacgccaat tgggtcagcg agagcccact
      361 gcccagagct cgcgcgcagt acacgctcgg ttcacgcagc gtacccggcg cggtcaagct
      421 cggcagctcg ggaaacacgt agaggctcgg caccttttga ccaaacaacc gcgccgccca
      481 gttcagagtc ttcgcgaaca tcgcggtgct acgttcggca tcgtgcaagt aagagggatc
      541 gggctcctga tagaccttct tgtgtttgag gaaaccgatc ccgagcgcgg catccgcgac
      601 cgcgtccgcg cgcgccagca ccgagagtag cggcaggagc aacaccgtcg cgctggttgg
      661 gcacagcgct ccgaagaact cggcgtcgct gacggtgccc cgcggcatca acaagccatc
      721 tggtttatgc tgatgcgcga gtagggattc gttgatgtca gcgtcgccca acgcttcgag
      781 gacgctggcc gcctgccacg cgccgtcagc cgcgcccacg ctttcgaaga gggcttgcgc
      841 ccggcggtag gtagcggccc gcaagggagc aatttgagct gccgcctggc agtgcaatag
      901 tgcgcgcgct ggagcggaag cttgcgtgca ggcctccacg agttgttccc gcaagtctag
      961 atctctagga aaacgcgcca agcacgcctc ccaggccagc gctgcgcgtg ccgggcttac
     1021 ctggcgctcc cggacgagca cggcgagctc cgccgagatc cgggacagct catcggagtc
     1081 gacggctcga agcagcgcat tcgcgtaata tgacaacagc tccgcggctc gagccctcgc
     1141 cgcgtcacgc ggggagaccg actcatgcgg gggtgcctcg gtgggcacag cgtctgcggc
     1201 cacgggcatt gcggcgctga aggcgtgggc tgccggcgtg gccgcgtgct cggtttgcgc
     1261 aaacggcaga atggcttgct ccggaccgga ccaaccgcca ggagttgcaa gcccgggcgc
     1321 gggttggaac gcgctccgtt ccaggccgcc ttgttgctcg acggctccgg gctgctcgag
     1381 gactccgggt tgctcgcccg gctgccctga gcttcgtggc tctagagtac tctgctcgaa
     1441 cgctcccggg ggcgttgctc cgcgcgcaag cgccggagtg ggttcctccc gcacgggctc
     1501 cggttgggct tcgaagccca gcaaggccgc caaatcagca tctaggttgg gcaggtcagc
     1561 aaagctcggc aaaggcgctg aagcttcggc tcgcaccttg cgaggtccct cggcccgagc
     1621 ggccctctgc ggaggtacgc cgggctgcag gattgacagc cggggctcca tacctccgaa
     1681 caatcccgtg gccgggatct cctggaacaa cgctggctcg gcacgcaacg cttcgtacag
     1741 gcgttcgatg tccacggctt cgcccgcttc gcgactcacg gcgagccaat cgagcgcagt
     1801 caacatcaac cccgtgaacg ccgagcctcc gaacgaatcc ggcacgctgc tgtctcgcac
     1861 cgcaaccaac gcggaaatcg acggcacgcc ctcggtcagg gcttctccaa tccccgtgac
     1921 gaccgcatcc aaactttgac ccgaatcgac gagagccgcg gcatcgacga tcagacaggc
     1981 gcgatggctg aaccgggcaa atagcccgcg caggcgccgc agggagaagg ttccaagccg
     2041 ctcgccatcc aagagcaagg caggtccccg ctcctgcgac aacaccgtgt atcccgaaaa
     2101 atagacgagc agctcaccga caagccccgc ctggcgcccc agatgatgct ccaacgtttc
     2161 cgccaggccg cgctccgcgg ctacccgcac cacctcgaag cccgcctcgg gctcggcaag
     2221 tcgcctgccc accagttctc cgtcgatcgc cgcggacggc aaaaccggaa gctttccact
     2281 tgtttcgtac ggcgaaccaa caatgaaggc gagtcgcatg gggaacgagc cggacaactc
     2341 gcaaaccata ctttcgatcg cgcgatctgc ccagcctcgc cactgagcgc ttgcgctatg
     2401 ccggttgggt cacgcctcca cccaaacaat ctgacctgaa agttccagat cgaccctttg
     2461 acgaggcgcc cgctccaaca agctattggc ctcgcgcgag cgtgcgcccc gtacgcggct
     2521 caccgtgaga tgcagcgggc ctccgtcgaa tggtcggtgt gtgctgccgg ccatctcgag
     2581 cgccagcgcc tgcagccttt cgtcgcagac ctgggagacg gcacgcaaag ccacccgctc
     2641 cccgggccga tgtccccccg gaacccactc gggagcgaag cgatgtggat cgactcggtg
     2701 ggccagcgtg acgtggtccc ctcgaacgac cggcagcagt gacatgcgcc ggacacaatc
     2761 cacactgctg cgatccagca ccacccacag ggagcgcttg gcgagctccg cctccaggcg
     2821 cttgacgagc ctcagatgcg cctccgatcc gaacttctcg tgaagtagaa cgatgcgagc
     2881 cgcgaggcgc tgctctgaca accggcgcaa ccactcttct tccgactctt cttcgcgatg
     2941 ggcaacgtgc ggaagagccc gcaccacctc ctcgggaaac ccctccgcgc tgagctcctc
     3001 caagctcctg agtctcagcg cgtcctgcaa caaccacgtc aatcgctcca ggggcttgtc
     3061 cagtttgctc gcgacgcgca gcacactgaa gatcgcgggc tcgccgtgag catcgaagcg
     3121 gcctagattg gcctcggcgg ccaacatcgc ggcacgcccg ggcagccagt tggagatcgt
     3181 tcgttgcaaa tcctccgccg cttgatcgaa acgtcgcagt aagcgagcgt tgacccgtcc
     3241 gtggaagaca cggcgcaagc gcgcatgctg ctctgcgtag tctcccgggc gagtcgcgag
     3301 cctcgagagc agtgattcgc ccgctgcttc cgtttccacg agcgaagacc aattgtgaat
     3361 cttgtctgca caactcacgg gtagcgccaa ctcggcttca ttccccgctg cggccaacct
     3421 cgccgcgtac tctgcgaacc gctccgcctt gggccgctta cgacccgaaa cgtccgtctt
     3481 gctctccgtc agcaactcca cggcagcgac gacttcgggt ggcatttcgc gccgcaatcg
     3541 atccgcgctc gcgggttgat cctcgagcac gtcgtgcagg taacccacgg cgagaatgag
     3601 agggtccgag tagccgtggt cgagcaagat cttctcgacc cccgacaagt gctcgaagta
     3661 cggacgtccg ccggccttgc gtagctgccc tcgatgcgcg tgctctgcca acgcgcgggc
     3721 gcgggccaga acaggtacgt cctccggcat gttctcgagc atatcccgag gcggcggcga
     3781 cggctcaaaa ctgaccgcgc atgcccaacg agctcggccc cacccagacc tctggaggcc
     3841 ggctggcagt cttctcgttt tggcgcgtac cgagcaccag aagggtggca ccggtggcca
     3901 agccgacacc accgaccacg aaaccaacca gagacaacgt tttgcgcgac tggcccgaat
     3961 ctcccagttc gttgcggcga tcctgctggg cttgggtcaa gtaacagggg aattctgggc
     4021 acatctcgtt cgcgtcgtcg aacctcgact tcgcgctcag cgcgagccac gtccccgccc
     4081 cgatcccaac cacacctaca ccaaaagccg catacgagag cgtccgcagc aagggccgac
     4141 ggtcggactc gagtgatgac cccgggacca ggacatcgga ccgagcgttg gacgaggaca
     4201 tcggtggagg cgcgatcgca accgcgtccg cggcttggaa ctccagcggg tacaccagcc
     4261 tttgctgcgg ctccgcgtcc aattccagca ctatatcttt gaagccggcc aggctggcgg
     4321 tgatgcgatg gtgacctgcc cgaacgccaa tctcgagggt tgagtcgatg gggccatagc
     4381 ggttcacgac gctggagccg ctcgccaacg aacgttcgtc caccagacgc gctcccgtgc
     4441 ggctcgcgtt gaaggcaatg cgcacgacgc cagtcttgag tgtctgcaga tcccgctcgg
     4501 tttgcgcgcg ctcctccgga tccagctgct tgccgccttc gctcaggtag cgcgcgaagg
     4561 cttcgatcgc ctccccgtcg cgctcgagct tcatcgccgt aatgccgagg ttacccaaga
     4621 tcttccaaga tggtgaggcg gcgtacgcgg cacggaactc acgatacgcc tcttcgtagc
     4681 gggctccgtc tggatcctgg agcagattca cgccggcgct gaaatgtgcg cgcgcctcct
     4741 ccgagacggc gacctccgcg gcgtgagccg acatgctcgc ggtgacaacc cagaactgaa
     4801 gtccgacgca aagccagcgc gctttcatgg tcctccactt tagaacctcc tccgcgaagt
     4861 tcgctatggc gcttcaccgt gcccgatatc ggagcgaggc ccgggagcaa ttcccatgag
     4921 tcggccgcaa cccgagtcgg ccatgggacc tgcgcacgcg aggctcagcc tccggcaagt
     4981 aagtaccgag tcgaacggga gtcgagtagg ttgcggacca tgcctgttcg ctcgcctcta
     5041 ccaacctggt tccgcgcctg ttccgtgggt tgtctgctca cggccgcgct cgccaccagc
     5101 gcgttcggcg ccgcacctcc cgtccccgct gcgcccgctg aggccaatct gcagatcccg
     5161 ttcgagaagt acttactgaa caacgggcta gaggtcatcc tgcatcagga ccgatccttg
     5221 ccgctggttg cggtcaacct ctggtaccac gtgggccccg ccaacgagcc ccccggacgc
     5281 tcgggctttg cgcatctttt cgagcacctg atgttcaagg gctcacgcca cgtcgggcgg
     5341 gacttcgaca agttgctcga agccgccggt gccaccaacg tgaatggcac taccttctgg
     5401 gaccgcacca actacttcga gacggtgccg cgcgagcacc tggagctggc gctctggatc
     5461 gagagcgatc ggatgggcta catgctggat ggcctggacc aagcgacgct cgatgccgag
     5521 cgcgacgtcg tcaagaacga gcgtcgtcag agcttcgaaa actcgcctta cggcaagagc
     5581 tccctggtct tgctcaacac tttgttcccg cagggcaccg cctatcacgg cgcggtgatc
     5641 ggctcgatgg aggacctttc gcgcgcttcg ctcagcgacg tacgcgagtt ctttcacacc
     5701 tactacgcgc ccggcaatgc cacgctcaca ctggcgggtg acttcgacgt agcgcaagcc
     5761 aaagcttgga tcaccaagta cttctcgacc ttggcggctc gccctcgccc cgcctctacc
     5821 gcccaaccga tctctgcggt aactcggtcg gagcatctga ccgtcaaaga gccggtcgag
     5881 ctcacccgcg tgtcctttgg acacgtcact cctccggctt attcgagcga cgatcccgtg
     5941 ctcgaggtca cggccgcaat tctggcggga ggcaaggcca cgcgccttta ccgcgcgctg
     6001 gtactggaag ggaagctcgc ctcggaggtc gacgccgcgg tggattcgaa tcagctcgcg
     6061 agtaacttca cgctgaccgc taccgttgcc tccgggaaga gcgcgacgca ggtcgaacgc
     6121 gccatgcaga ccgtgctcga ccagctcgcc gagcgaggcc ccactgcggc cgagctcgac
     6181 cgagctcggc gccgcattct cctcaatgtg ctcggcaatc tggagctgtt gaacggctca
     6241 ggtggcgaag ccgggcgcgc gggaattttg caacgcttca atcactacct gaaagatcca
     6301 ggttatttgc cgaaatgggt ggagcaaatt cgggcggtaa agggcgagga tgtgtcgcgc
     6361 gtgattcgag ctcatctatc ctccgaacat cgcatcgtgg tgaccacgtt gcccgaggtg
     6421 acgccgtgaa cgcgctcgct acccggcagt ccgcgtggct cgtcttggtc gggagcctgc
     6481 tctttcaggc ggcgtgttcg ccggctcggg ccaccggccc gagcgcgaaa cctgcaccca
     6541 cggccgtggt cccggtctcc gccggaaacc ccgatccgga gccatttcgc gcacgaaagc
     6601 cgcaacctgg ctcgccgggg gagttcactt acccgacgcc caaggtggtg aagctcccga
     6661 gcgggctctc ggtctatctc gtccaacgcc cggcgcgcgt cgtcgccatc gagttcaacg
     6721 tgcggcacgg ttcagcctcg gatccggagg gtaagagcgg tgcggccgcc ttgtgtgcgc
     6781 gcatgctgac cgaagccacg cggcgcaagt ctgcgctggc cttatccgag gccgtggagg
     6841 cgctcggcac gaatctgcag gcctcggcct cgcgagatag ctccaacgtg gcactcagcg
     6901 cgctgccggg cgacgtggaa gccgcgctcg cgctgctcgc agaggtgatc accgagccgg
     6961 cctttgcgcc cgccgacttc gagcgcgtcc gcgcggagtg gttggatggt ttgcgagcgg
     7021 aacgtcaaga tccccagcga ttggccattc tgctcgccaa tcgctggctt cttgggccgc
     7081 ggcacggagc accgagcggc ggaagacttg gcgatgtgca gaagctcacc gttggtgatc
     7141 tgcgggcgtt ccaccgccaa gcatacacac ccgacaactc cgcgctcatg ctggtcggga
     7201 acttcgatga aaacgaggtg atgaagcgtg ttcaggcgtt gttcggcaac ctacgcggca
     7261 aagacgcgat tgctcctccg ccccctgtgc cagaatcgcc gctgacccaa acgcaaatcc
     7321 gcgtcctgga tcgcaaggac gccgtgcaga gcgcgatcgc ggtgattcag ccgttccccc
     7381 gccgcagcga ggctggcttc gaagctcgcc agatcttggg tcgcctgctc ggtggcttgt
     7441 tcacttcccg actgaacacg cgcttgcgcg aggagcacgc ttatacgtac ggcgccgccg
     7501 cgacgccctg ggccgcgaag catagcggag cgctgctcgt gtacacgagc gtgcggacgg
     7561 atgtcaccgc accggcgctg accgatattc tcggggagct cgatcgcacg cgcgatccca
     7621 agctcggcgc gcccgtgggc gaagaagaaa tgcagagagc gaaggctgac ctgatcttct
     7681 cgttgggcgc gagcctcgag cacccgagtc gaatcgccga tcggatcggc gcctccttca
     7741 tcgacggtca ggcccccgag tacgacgcca attatccggc actcattcgg gccctccctc
     7801 tccccgccgt tcaaatgagt ggagccgcgc tgtccacggc cagccgcgtc gtgatcgttg
     7861 gggacaaggt ccagatcaca gagagcctgt ccaagttagg catgccggtg gagagtgtgc
     7921 ccgagggctt actcgagaac tgaggtttgt ggaaacagag ctcggtggat tgagctcggt
     7981 agattga
//
LOCUS       DASTKU010000260         2934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_719535, whole genome shotgun sequence.
ACCESSION   DASTKU010000260 DASTKU010000000
VERSION     DASTKU010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719535"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..1292
                     /locus_tag="VFQ61_09355"
     CDS             285..1292
                     /locus_tag="VFQ61_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HET9954701.1"
                     /translation="MQMTKTARVVLGLLLIGSLTSCKVIENLRKGQGLKGNVASASGE
                     TAASREEQKDAELSLKLEGYIDCLNFASADASRARLSYLMHVDEARGPTGKEANLWVP
                     EINTSRCLPGIEKAKTLPPQLPELETLAANYRAALDALLPLVKGAHDYYDHKDFKDDN
                     WQRGKQLHPELIAAFAKFNEAHVPFNKQVTELNDAVSSRRLLRLAHLPEARFQFLIEK
                     ALDDAKSLVKLADAEFSKLDVVAFGERLDAYEKSFNEFRSYEGAHPEEASRITALTVF
                     RSSAEDYLKAAKELMRRKRDNRGFEAVKGNPENVDGHPAQVVARYNRVVNASNSLFFK
                     R"
     gene            complement(1341..2264)
                     /locus_tag="VFQ61_09360"
     CDS             complement(1341..2264)
                     /locus_tag="VFQ61_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954702.1"
                     /translation="MMEPETRSAFLMRVVQETLDSIVSPNVRDALLQEALLLGGEAEL
                     PTQKQRFPAFLDGPLRKALTRALGPELGQSVVSELERLTFIDSEPIRESTPKPTARGR
                     RHPLATLPAGAAASEPIASPPRDDLARAATAPTWPVRAPVAEALPPEPRIPEPRIPDA
                     RVPMSADYPAGTAGVLGFASEPQVTSRRLPMVFLVTRSAELVQRFGDWLDPRVIVLRV
                     SRLIDLMINIQDAGPRRMVVVLDAADPPMRLEAMAAVHEELPGNIKVVLWGLRSDLPD
                     VVSELFPRSREWLVCSEPVPLSEAVARCVSG"
     gene            complement(2506..>2934)
                     /locus_tag="VFQ61_09365"
     CDS             complement(2506..>2934)
                     /locus_tag="VFQ61_09365"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HET9954703.1"
                     /translation="SGVNARTELDLTFTNSPNQPGMFVYDDQEFFPIDGQLLGNEDRP
                     HNFHFTLAAILAFRYVGGEVFRFTGDDDLWVFVNRRLAIDLGGTHLSASGEFSLDKRA
                     DELGLGLGGVYPLHLFFAERHTRESHFRVETSLADPGTCE"
BASE COUNT          600 a          929 c          932 g          473 t
ORIGIN      
        1 gagagcctgg atttccccat caagccgtgc gcgtcgcttt gctcgctggc agcacggatc
       61 ccctgaacct gatggcattc cggaaagcgc cgctcaaaga gcgcaccatt agcccagccc
      121 cttgagcccg aaggcgagcg ctgggcccgg aggcgagcgc tgagcccgaa ggcgatcgcc
      181 ggaacggcgt tcggtgcgcc cccagactgg agagccgccg aagctcacag gaagcccctc
      241 agcgtctacg gagtgcgata ggctctgacc agcacggatt tggaatgcaa atgacgaaaa
      301 ccgcgcgcgt cgtcttgggc ctgttgctga tcgggagctt gacgagctgc aaagtgatcg
      361 agaacctgcg caagggtcag gggctaaaag gcaacgtggc gagcgcatcc ggggaaaccg
      421 cggcgtctcg ggaagagcaa aaggacgccg agctctcgtt gaagttggag ggctacatcg
      481 actgcctgaa cttcgcgagc gcggacgcct cgcgtgcccg cttgtcctat ctgatgcacg
      541 tcgatgaagc gcgggggccc accggcaaag aggcgaacct ctgggtgccg gagatcaaca
      601 cgagccgctg cttgccaggc atcgagaagg ccaaaacctt gccgccccaa ctaccggagc
      661 tcgagacttt ggcggccaat tatcgagccg cgttggacgc tctgctgccg ctcgtgaagg
      721 gcgcgcacga ctattacgac cacaaggatt tcaaggacga caattggcag cgcggcaagc
      781 agctccatcc cgagctgatc gccgcattcg cgaagttcaa cgaggcgcac gtccccttca
      841 acaagcaagt caccgagctg aatgacgccg tctcctctcg gcgcctgctc cgcctcgccc
      901 acctgcccga ggcgcgcttt caattcttga ttgaaaaggc gctcgacgac gcgaagagcc
      961 tagtgaagct ggccgacgcg gagttctcga agctcgacgt ggttgcgttc ggagagcggc
     1021 tagacgctta cgaaaagagc ttcaacgagt tccgcagcta cgagggcgcg cacccggaag
     1081 aggcctcgcg catcaccgcg ctgaccgtgt tcaggagcag cgcagaagac tatctgaagg
     1141 ccgccaaaga gctgatgcgt cgcaaacgcg acaatcgtgg attcgaagcg gtcaaaggca
     1201 accccgagaa cgtggacgga catccggcgc aagtcgtggc gcggtacaac cgcgtggtaa
     1261 acgcctccaa cagcttgttt ttcaagcgct gaacgcgacc aattaccgcc gcgcacgcga
     1321 acacgcccag ccgaacacgc tcagccggac acgcaacgtg cgaccgcctc cgagagcggc
     1381 acgggctcgg agcagactag ccactcgcgg ctgcggggga acagttcact gaccacatcc
     1441 ggcaagtcac tgcgcagccc ccaaagcacc actttgatgt tgccaggcaa ctcttcgtgc
     1501 acggcggcca ttgcctccaa acgcattggc ggatcggccg cgtcgagcac gaccaccatg
     1561 cgccgcgggc cggcgtcttg gatgttgatc atcaagtcga tcaggcgcga gacgcgcagc
     1621 acgatcacgc gcggatcgag ccaatccccg aagcgttgca cgagctccgc gctgcgagtg
     1681 actagaaaaa ccatcggcaa acggcggctc gtcacctgcg gctcgctcgc gaaacccagc
     1741 acaccggccg ttcctgcggg atagtcagcc gacatcggca cgcgtgcgtc cggaatgcgc
     1801 ggctcgggga tgcggggctc cggaggcaac gcttccgcga caggtgcacg caccggccaa
     1861 gtcggggcgg tggcggcgcg agccaaatca tccctcgggg gagacgcgat gggctcagac
     1921 gcggccgcgc ccgccggcaa cgtcgcgagc ggatgtctgc ggccgcgcgc cgtgggctta
     1981 ggtgtcgact ctcgaatcgg ttcgctgtcg ataaacgtca gccgctcgag ctcgctcacg
     2041 acggattgcc cgagctcggg cccgagcgcg cgggtgagcg cctttctcaa tggaccatcg
     2101 agaaacgctg gaaagcgctg cttttgcgta ggcagctcag cctccccacc gagcagcagc
     2161 gcctcttgca gcaaggcgtc gcgcacgttc gggctgacga tgctgtcgag cgtctcctgc
     2221 acgacgcgca tcagaaatgc cgagcgggtc tccggttcca tcacggtcct taagcttacg
     2281 aacctcggat ccgagcacga tacacccccg tccgaaagag cgctagcggg ctcacggtcc
     2341 tcccgggttg agacgcggat ggtccgcaac gctcatgagc gcagatgtgc cctgctcagc
     2401 ttgcgcgggt acggatgggc gcgcgtgagc ttcttcgagc cgattgtgcg atctgtaagc
     2461 tgaccgcgct cgtgtcagct gatgtgctgt cttcgagcgt gagggctatt cgcaggtccc
     2521 cggatcggcg aggctggttt ctacgcggaa gtgggactcg cgcgtgtggc gctctgcaaa
     2581 aaacaggtgg agcgggtata cgccaccgag acctaggccg agttcgtcgg cgcgcttgtc
     2641 caaggaaaac tccccggaag cgctcaggtg agtgccgccc aggtcgatcg cgagccgccg
     2701 gttcacgaac acccacaagt cgtcgtcacc cgtgaaccgg aacacttctc cgccgacgta
     2761 tcgaaaggcc agaatggccg ccaacgtgaa atggaagttg tggggacggt cctcgtttcc
     2821 gagcaattga ccgtcgatcg gaaagaattc ctgatcgtcg taaacgaaca taccaggctg
     2881 attgggcgag ttcgtgaacg tcaaatcgag ctcggtgcgt gcgttgacgc ccga
//
LOCUS       DASTKU010000261         8013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_497945, whole genome shotgun sequence.
ACCESSION   DASTKU010000261 DASTKU010000000
VERSION     DASTKU010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497945"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VFQ61_09370"
     CDS             complement(<1..705)
                     /locus_tag="VFQ61_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954704.1"
                     /translation="MRRRNWSLSSSLVLTARSRLLRLGAAAYALLAPAPAAAQTDINP
                     PLPNVLLLVDTSGSMEYKVGSSSFPNCDPTGSQSSEKSRWVDLIEVLTGSIRNYRCER
                     INRAQASFGSGEYGTGGPLGLRSYDYLYENPFHRPLSGTCAVGPGTLPTNPYQFPDTA
                     LKYHAYNDNNSTCTFNQARDGILDAFENSIRFGLMTFDPNTDPSTGLTGTGGADFAGG
                     IKGTWSYVWGNAAKANP"
     gene            complement(742..1452)
                     /locus_tag="VFQ61_09375"
     CDS             complement(742..1452)
                     /locus_tag="VFQ61_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954705.1"
                     /translation="MENPHPHLELLPQRQASTPARRRVKRGERGAAVFVVVMAVTLLT
                     AIGLFAAHSATLVDQAAGYARLARQSQHMAEFGTQLATAELGSGAAEAYLQKMSQRDV
                     TCTANAGTTGAPCYVFFKADFAARTNALVGDVLFRDQSGDVIDVIGSHADPSNLTNTV
                     GDVTSEIVVELTEPGPVGVPVAGSDLGNNLSFRYQKVTVTSTAQMRPAGFPTCTEQVA
                     SLTGQQSMRAHIIVGPVH"
     gene            complement(1452..2774)
                     /locus_tag="VFQ61_09380"
     CDS             complement(1452..2774)
                     /locus_tag="VFQ61_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9954706.1"
                     /translation="MKARGELEWGNRASRRGFTLIELLVSLTGGLFVSLAVFALARDA
                     SRFYQREGRLASATLSGIVGFERLRADIARAGFLSTPNVSNDPFVCSRPNASSPTLLR
                     DLSSLRVVNQGSPTNAILQANSLAPDSLLLMGSYSSPDEFIVRDISREDSTGGAVVTL
                     LPTSAAMARLGFLSAPPGQRKALLQGVFATGRALRIVDNEGRQHFGVIQSANVDPTGT
                     IPEVRLSAQPALEFRPDSSRLCGLKAGERGARVNVINFIRYDVRNLNTQSSFDGGKNK
                     NYAALYTASKADALSEQETARTELVRTELDVNENPLTLTQAVSDTQNLSTNLEELVAE
                     YAVDFQLEAGVAIDPPPNPSLQHFTDTQNQFAQYVGKPTAGASPERLRSVRVRLSLRS
                     READRAMPLVAAGGLYRVGLGSDGKAPYARVRSLQADVMLNNNAHVSW"
     gene            complement(2771..3445)
                     /locus_tag="VFQ61_09385"
     CDS             complement(2771..3445)
                     /locus_tag="VFQ61_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9954707.1"
                     /translation="MKMLRRPFKRSTPEAPRPCRRRGARGFTFVELLLSLTILGIGVS
                     GVIAMQKVTVTSNQHAKNLAIATQVAQAWIDALQADAVQWNHPSSSNPVSDLPTTDWL
                     SLAPAEGSGPNRDWVRPTWNARRQFGPGFTAQGRPVDPATSPNDVRFCTHIRLSWLYP
                     QNGVITGNGLLRAEVRVFWLREGGSALGNVSICDPSVNPNAVGAETRRYHQIYEVTAV
                     RENTAL"
     gene            complement(3442..4167)
                     /locus_tag="VFQ61_09390"
     CDS             complement(3442..4167)
                     /locus_tag="VFQ61_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET9954708.1"
                     /translation="MRRLPPASITPLPPFSRSAAPAGLPWVRSFERVRGRPARWSEVS
                     GARGFTLVELMVVVIIVALVATIAVPSAVERMRERRSQEAAQKIAAIYRNARIRAMGR
                     GAAVLVRYTTTKGFQILEATRGGASGCAREPFRSCVTNGTEDLWSTSQAQPAMADFDP
                     TRRSEYSNLTVNAVSSTTSSAPDSYLDVCFTPIGKAYFRNAAAPQPLMPMVGVVTFDV
                     KRGNNGLKRTVTVLPNGTSRLGL"
     gene            4647..5759
                     /locus_tag="VFQ61_09395"
     CDS             4647..5759
                     /locus_tag="VFQ61_09395"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HET9954709.1"
                     /translation="MSVLRACLGLLIGSTLAVACKRSAPPPSEGAPPPPPVVSSQPGV
                     CAGGGGTLKDSVSSAFFPRVAQDYCIDPNSEARTFGEQGQAPLDAVCDLFDGECEVYK
                     QFGLKRVVTLQYVDGKGSPGNVTVTLSRFASPEAAFGFFTKRVVAEGDPLEVAPAPLD
                     AGAAAALGSGMAYVVRGELVAELRYVHELESPDQIKASSAKILPVLAKALGEKLPGDP
                     APPPSVVRLPKEHRIPSGVSYVYGDLLDVSGAGRGAVGYYQEGSARYRIFASVRPDEE
                     SAKDVIKTLRKHEGAKGIKDSPIDALTLFVRESEAAPRSEWLVGRMGSIVAGVGDEPF
                     SLGAAKANTERLNDAQKMEKLRAVLAGQKAAPAKTP"
     gene            5878..7023
                     /locus_tag="VFQ61_09400"
     CDS             5878..7023
                     /locus_tag="VFQ61_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9954710.1"
                     /translation="MDFELPETHRLLRETVRQFAEREVRPHAQSWDEEERFPHEIVPR
                     LAELGLLGIRIPEEYSGSGLDMLAYAVCVEEIARVDGSLALTIASHNGLGTGHILAFG
                     NEAQKRKYLPKAAHGEWLAAWALTEPGSGSDSAALKTSARRDGDDWIIRGTKLFITQG
                     SVGGFCVVLARTRYDVPAQRGITAFIVETNTPGYSASRKLKKYGCRASDTVEVTLDDV
                     RVSDAQRLGEVDQGFVDTMRILDSGRVSIAAMALGLGRGALDLAVRYARERTAFGKPI
                     SEFQSLAFMLADGKTALEAAAWLTYRAANLADHGERFSQEASMAKLFASEAATKVCND
                     ALQIHGGYGYTREFEIERYLRDVKLCEIGEGTSQIQRMVIAKHLLRG"
     gene            complement(7030..7512)
                     /locus_tag="VFQ61_09405"
     CDS             complement(7030..7512)
                     /locus_tag="VFQ61_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9954711.1"
                     /translation="MIRHLLLGCWLATGCVVTTIEGPAEGPRITPRPEQTKASVSEET
                     TAPAEALGPKSIAARHLLVSYAGAMRAGATVTRTKEEAQKRAEEALGRARAGEDFEKL
                     VAEYSDEPGAADRGGDLGTFERDKMVKQFSDAAFKLNPGELSAVVETPFGFHVIQRTE
                     "
     gene            complement(7509..>8013)
                     /locus_tag="VFQ61_09410"
     CDS             complement(7509..>8013)
                     /locus_tag="VFQ61_09410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9954712.1"
                     /translation="ALVLLGTAGGLGLLGGMAPPRRAAAEEVIQPSKPATATDKQPER
                     QAAPPSPAAAAVAQDSERIEASHLLVAYQGALRVDPKVTRTKEEAKKRAQEALAKAKK
                     GAAFDKLVAEYSDEPRAAERKGALGSFTRRQMVKPFADAAFALKPGQISDVVETNFGF
                     HVIQRTK"
BASE COUNT         1405 a         2507 c         2633 g         1468 t
ORIGIN      
        1 cgggttggct ttggccgcat tgccccagac gtagctccac gtacccttga ttccacccgc
       61 gaagtcggcg cccccggtgc ccgtcaatcc agtgctcggg tcggtgttcg ggtcgaacgt
      121 catgagcccg aagcggatcg agttttcgaa cgcgtctagg atgccgtcgc gcgcctgatt
      181 gaacgtacaa gtcgagttgt tgtcgttgta ggcgtggtac ttgagagcgg tatcgggaaa
      241 ttgataagga ttggttggca gcgtgccggg gcccaccgca caagtgccgc tcaggggtcg
      301 gtgaaatggg ttctcgtaca gatagtcgta cgagcgcaac ccgagcggcc ctcccgtccc
      361 atactcaccg ctaccgaagc tcgcttgagc gcgattgata cgctcgcagc ggtagttccg
      421 aatgctgccc gtaagcacct cgatcaaatc aacccagcgg ctcttttcgc tgctttggct
      481 gccggtaggg tcacagttcg ggaatgagct cgaacccacc ttgtactcca tcgatcccga
      541 ggtatcgacg agcaacagga cgttgggaag gggagggttg atgtcggtct gcgccgccgc
      601 aggcgcgggc gccaacagcg cataggccgc ggctcccagt cgcagtaagc ggctgcgtgc
      661 ggtcagcacc aacgaggacg agagagacca gtttcggcga cgcatggcta aaccctagtt
      721 tcgaagacta gcggatgagg ctcagtgcac gggtcccacg atgatgtggg cacgcatgga
      781 ttgttgcccc gtcaggctgg cgacttgctc cgtgcaggtg gggaaacctg ccgggcgcat
      841 ctgcgccgtg ctggtgacgg tgactttctg atatcggaag ctcaggttgt tgcccaagtc
      901 gctacccgcg accggtacgc cgacagggcc cggctcggtc agctcgacta cgatctcact
      961 cgtcacgtcc ccgacggtat tggtgaggtt gctgggatcg gcgtggcttc cgatcacgtc
     1021 gatcacgtcc ccggattgat cgcggaacag cacgtcgccg accagggcgt tggttcgcgc
     1081 ggcaaaatca gccttgaaga acacgtaaca aggagcaccg gtagtgcccg cgttcgcggt
     1141 gcaagtcacg tcgcgttggc tcatcttctg caagtacgct tccgcggcac ccgagccgag
     1201 ctcggcggtg gcgagctgag tgccgaactc ggccatatgc tggctttgcc gggcgaggcg
     1261 ggcataacca gcagcttgat cgaccaaggt agccgagtga gcggcgaaca agccaattgc
     1321 ggtcagcagc gtgaccgcca tcaccaccac gaacacggcc gcgccgcgtt cgccccgctt
     1381 cacgcgccgg cgagccggtg tcgacgcttg acgctgcggg agcaattcca aatgaggatg
     1441 ggggttctcc atcaccagct cacatgggcg ttgttgttga gcatcacgtc cgcttgcagg
     1501 ctccggaccc gagcgtaagg cgcctttccg tcgctgccga ggccgacgcg atacaggccc
     1561 cccgccgcga ccaagggcat cgcccgatcg gcttctcgag agcgcagcga caggcgtact
     1621 cgcaccgagc gcagtcgctc tggactggcg cccgccgtcg gtttgcctac gtactgcgca
     1681 aactgattct gcgtatcggt gaagtgttgc aggctggggt tgggcggagg atcgatcgcg
     1741 accccggcct cgagctggaa atcgacggcg tactcggcga cgagctcctc gaggttggtg
     1801 gacagattct gggtgtccga gacggcttgc gtcagagtga gggggttctc gttcacgtcg
     1861 agctccgtgc gcacgagctc cgtgcgcgcg gtttcttgct ccgagagcgc gtcggctttg
     1921 ctcgcggtgt agagcgccgc gtaattcttg ttcttgccgc cgtcgaagct cgattgcgtg
     1981 ttcaggttcc gaacgtcgta acgaatgaag ttgatgacgt tgacccgcgc tccgcgctcg
     2041 cctgccttca acccgcacaa ccgcgaagag tccggtcgga actcgagggc cggctgcgcc
     2101 gacagccgaa cttcggggat ggttccggtc ggatcgacgt tggcggactg aatcacccca
     2161 aagtgttggc gtccttcgtt gtcgacgatg cgcagcgcgc gccccgtcgc aaacacgcct
     2221 tgcagcaaag ctttgcgctg accgggaggc gccgacaaga acccgagacg cgccatggcc
     2281 gcactagtgg gtagcagggt gacgacggcc ccaccggtgc tgtcttcacg gctgatgtcg
     2341 cgcacgatga actcgtcggg agaactgtag gagcccatca gcagcaaact gtccggggcg
     2401 aggctgttgg cctgcaggat cgcattggtc ggagagcctt ggttgacgac ccgcaagctc
     2461 gacagatctc tcagtagagt tggcgagctc gcgttcgggc gcgagcaaac gaacggatcg
     2521 ttactgacgt tcggcgtcga gaggaacccc gcgcgcgcga tgtccgcgcg caaacgctcg
     2581 aagcccacga tcccgctgag cgtagccgag gcgaggcgcc cctcgcgctg gtagaagcgc
     2641 gaagcatctc gggccagcgc gaacacggcc agggagacga agaggccgcc ggtcagcgaa
     2701 accagcagct cgatcaacgt gaagccccgc cggctcgcgc ggttgcccca ttcgagttcg
     2761 ccccgggcct tcatagcgcg gtgttctccc ggacggcggt gacctcgtaa atttggtggt
     2821 aacgtcgtgt ctcggccccg accgcgttcg ggttcaccga gggatcgcag atcgacacgt
     2881 tcccgagtgc gctgccccct tcacgcagcc agaacactcg aacctcggcg cgcagcaggc
     2941 cattgccggt gatgacgcca ttctgcgggt agagccacga caaacggatg tgagtgcaga
     3001 agcgcacgtc gttcggcgaa gtagcggggt ccaccgggcg gccttgtgcc gtgaagcccg
     3061 gcccgaattg gcgacgtgcg ttccaagtgg gacgaaccca gtcgcggttc ggtccgctgc
     3121 cttcggcggg cgccaagctc aaccaatcgg tggtcgggag atcactcacg gggttcgaag
     3181 aagaggggtg attccattga accgcgtcgg cttgcagcgc atcgatccag gcctgagcca
     3241 cctgcgtggc gatcgccagg ttcttggcgt gctggttcga cgtgacggtg accttctgca
     3301 tcgcgatgac tcccgagacg ccgatgccca agatggtcag cgacaacaag agctcgacga
     3361 aggtgaagcc gcgcgcccct cggcgcctgc acggccgcgg ggcttccggc gtgctccgct
     3421 tgaatggcct tcgaagcatc ttcatagtcc gagcctcgaa gtgccgttcg gcagcaccgt
     3481 gaccgtgcgc ttcaagccgt tgttgccgcg cttcacgtcg aaggtgacga cgcccaccat
     3541 gggcatcaac ggttgaggag cggctgcatt gcggaagtag gccttgccga tgggtgtgaa
     3601 gcacacgtcg aggtagctat ccggcgccga gctggtggtg ctcgagaccg cgttgacggt
     3661 caaattgctg tactcgctgc ggcgtgtagg gtcgaagtcc gccatcgcgg gttgcgcctg
     3721 actggtactc cacaaatcct ccgtgccgtt cgtcacgcag ctgcggaagg gttcacgtgc
     3781 acagcccgag gcaccgcctc gcgtggcctc caagatctgg aagcccttcg tcgtcgtgta
     3841 cctcaccaaa accgcggcgc cgcgacccat cgcccgtatc cgggcattgc ggtagatagc
     3901 cgcgatcttc tgcgcggcct cctgcgagcg gcgctcgcgc atgcgctcga ctgccgaggg
     3961 caccgcaatc gtggccacca gcgccacgat aatcacgacg accatcaact ccaccaacgt
     4021 aaagccgcgt gcgccgctaa cctcactcca acgcgctggg cgcccgcgga cgcgctcgaa
     4081 actgcgtacc cagggaagcc cggctggggc cgcagagcgc gagaacggag gtagcggtgt
     4141 gattgacgcg gggggtagac gacgcacggg gggcggccct aactgaaggt tttcggcgct
     4201 gcaattccac gctggagagc accgggacag tcggctgcgt ctctggaggc agcaaggttc
     4261 atacctgcct cggagcatgt tggtggctcg agttatcggc attttcacgc gcccgctagg
     4321 tcccgcttcg taaggcttgc gagactctgc aaagcttgcg gagtgggtcg cccgagccga
     4381 atgtaagctt tcagcccttg cggctgcccg aagcgaagcc gcttcgctcg ccaatgtagg
     4441 cggtctcccg ctcgaatcgg acggtttttg ctcgaaccgg cgctgcccac cagagctctt
     4501 gtcttttggc tttcgcgact ccaacccacg aggttcgcgt tttcgggttt gattgttcgg
     4561 atgggccggt ttatgtgcgc tgggctcgcg ctcgcgccgg tccgtttcgg atgggctcgc
     4621 gatacctcgt tcaccaggtg atccccatgt ctgttctgcg tgcctgcctt ggtttgctga
     4681 tcgggtcgac tcttgccgta gcttgcaagc gcagcgcgcc cccgccatcc gagggagctc
     4741 cacctccccc gcccgtggtg agctcgcagc ccggagtatg cgccggaggc ggcggaacgt
     4801 tgaaggactc ggtcagctcc gcgttcttcc cgcgcgtcgc tcaggattac tgcatcgatc
     4861 ccaacagcga agcccgcact ttcggggaac agggccaggc accgctcgac gccgtctgcg
     4921 accttttcga cggagagtgt gaggtttaca agcagtttgg tctgaagcgc gtcgtgacgc
     4981 tgcagtacgt tgacggcaaa ggctcgcccg ggaacgtcac cgtgacgctc tcgcgcttcg
     5041 cttcgcccga ggccgcgttt ggcttcttca ccaagcgcgt ggttgcggag ggcgatcccc
     5101 tcgaggtcgc gccggcccca ctcgacgcgg gcgcggccgc ggcgctgggc agcggaatgg
     5161 cttatgtggt gcgcggcgag ctcgtagctg agcttcgtta cgtccacgaa ctcgaatctc
     5221 ccgatcagat caaggccagc agcgccaaga tcctgccggt gctcgccaaa gcgctggggg
     5281 aaaagctgcc cggggatccc gcgcctccgc cttccgtggt tcgcttgccg aaggagcacc
     5341 gaatcccgtc aggtgtgtct tatgtgtacg gggacttgct agatgtatcc ggcgcaggac
     5401 ggggagccgt gggctattac caagagggca gcgcgcgtta ccgcattttc gctagcgtcc
     5461 gcccggacga ggagagcgcg aaagacgtga ttaagacgct gcgtaaacac gaaggggcca
     5521 agggcatcaa agattctccg atcgacgcgc ttacgctgtt cgtgcgcgag tccgaggccg
     5581 caccgcgcag tgaatggctg gtagggcgca tggggtcgat cgtggccggg gtaggggatg
     5641 agccgttctc gctcggcgcg gccaaggcga acacggagcg gctcaacgac gctcaaaaaa
     5701 tggagaaact ccgcgccgtg ttggcgggcc agaaagccgc gcccgccaag acaccctgaa
     5761 ggggtgcgcg gccgagctgg ggccgggcgg gctcctgcgc attttcgggt cagaatagct
     5821 cgaagcactt cagcacgcgg aagcctgagg ttcggcgttt ccgcctacgg atggaacgtg
     5881 gatttcgaac tacccgagac acaccgactg ctgcgagaaa ccgtacgtca atttgcggag
     5941 cgcgaggtac ggcctcatgc ccagagctgg gacgaggagg agcgcttccc gcacgagatc
     6001 gtgccgcgtt tggccgaact aggactgctc ggcattcgta tccccgaaga gtactccggc
     6061 tcggggctcg acatgttggc ctatgcagtt tgcgtcgaag agatcgcccg ggtcgatggc
     6121 tccctggcgc tgacgattgc tagccacaat gggctcggga cggggcacat cctggcgttc
     6181 gggaacgagg cccaaaagcg caagtacttg ccgaaagccg cgcacgggga gtggctggcg
     6241 gcctgggcgc tcaccgagcc tggctccgga tcggatagtg cggcgctgaa gacgtccgcc
     6301 cggcgcgacg gggacgattg gatcatccgc gggaccaagc ttttcatcac gcagggctcg
     6361 gtagggggct tctgcgtcgt gttggcgcgt acccgctatg acgtgcctgc gcagcgcggc
     6421 atcaccgcct tcatcgtgga gaccaacacg ccaggctaca gcgcgagccg caagctgaag
     6481 aagtacggct gtcgcgccag cgacaccgtc gaagtgacct tggacgatgt gcgcgtgtcc
     6541 gacgcgcagc ggctcggcga agtcgatcag ggcttcgtgg acaccatgcg cattctcgat
     6601 agcggtcgcg tatcgatcgc ggccatggcg ctcgggctcg ggcgaggcgc gctcgatctg
     6661 gccgtgcgct atgcgcgaga aaggacggcg ttcggcaagc ccatctcgga gttccaatcg
     6721 ctcgccttca tgctcgccga cggcaaaacc gcgctcgaag ccgcggcctg gctcacctat
     6781 cgagccgcga atctggccga ccatggcgag cgcttcagcc aagaggcctc gatggcgaag
     6841 ctcttcgcga gcgaagccgc aaccaaggtt tgcaacgacg cgctgcagat ccatggtggc
     6901 tacggctaca cgcgtgagtt cgaaatcgaa cgctatctca gggacgtgaa gctctgtgaa
     6961 atcggcgaag gaaccagcca gatccagcgc atggtgatcg caaagcacct gctgcgcggc
     7021 tgagagactc tactcggtcc gttgaatgac gtggaaaccg aacggcgtct ccaccactgc
     7081 cgacaactca cccgggttga gcttgaaggc ggcgtccgag aattgcttca ccatcttgtc
     7141 gcgttcgaac gtccctaaat ctccgccgcg gtcggcggct cccggctcat ccgagtattc
     7201 ggcgacgagc ttctcgaaat cctcgcccgc tcgagcccgg cctagcgcct cttccgctcg
     7261 cttttgggct tcttccttgg tgcgcgtgac cgtcgccccc gcccgcatcg cccccgcgta
     7321 agacacgagc agatgccgtg ccgcgatcga cttgggacca agcgcctccg cgggcgcggt
     7381 agtctcctcc gacaccgagg ccttggtttg ttcgggccgc ggtgtgatgc gcggcccctc
     7441 ggcagggcct tcgatggtgg taacgacgca gcccgtcgcg agccaacaac ccaaaagcag
     7501 atgacggatc acttggtgcg ctgaatgacg tgaaagccga aattggtctc gacgacgtcg
     7561 gaaatttggc cgggtttcag ggcgaaagcg gcgtcggcga acggcttgac catttgccga
     7621 cgagtgaaag agccgagtgc ccccttgcgc tccgccgcgc gcggctcatc cgagtactcg
     7681 gccacgagct tgtcgaaagc ggcgcccttc ttggcttttg ccagagcctc ctgtgcacgc
     7741 ttctttgctt cttctttggt gcgagtgact ttgggatcga cgcggagcgc accctgataa
     7801 gccacgagca agtggctagc ttcgatgcgc tcgctatctt gagcaaccgc ggccgcagcc
     7861 ggcgacgggg gcgcggcctg tcgctcgggt tgcttgtctg tcgcagtagc gggcttggat
     7921 ggctggatta cttcttccgc ggcggcgcgg cgcggaggcg ccatgccgcc gagcaggccc
     7981 aagccacccg ccgtgccgag caacaccaat gcg
//
LOCUS       DASTKU010000262        11613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_943281, whole genome shotgun sequence.
ACCESSION   DASTKU010000262 DASTKU010000000
VERSION     DASTKU010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11613
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943281"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..855)
                     /locus_tag="VFQ61_09415"
     CDS             complement(7..855)
                     /locus_tag="VFQ61_09415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET9954713.1"
                     /translation="MPLPLTELLQVLDQLAPLRLAEDWDNVGLLIDPRESGRELPISR
                     LLLTIDATPTVVSEAESLRAQCLLSYHPPLFRAKKRFSRAEDPVVFAAAHAGFAVYSP
                     HTALDAAPSGLTDWLAEGAGSGRLTALVPALTNEGGSTAISPPGHEPSPAIGAGRLLE
                     LDQPTPLGTILERLKQHLGLTQVRLAASAAHDHGASIRRIAFCAGSGGSLFERATADL
                     FVTGELGHHAVLAKLAQGQSVILCEHSNTERGYLPRYRQRLLDATRGEIEVLIAAADR
                     EPLQIV"
     gene            complement(910..2346)
                     /locus_tag="VFQ61_09420"
     CDS             complement(910..2346)
                     /locus_tag="VFQ61_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9954714.1"
                     /translation="MQKSPPRRYLGFSLIAACCTVLAGSCSKDDSAPLPSLPSGGSAG
                     TSSAGGSGAHDAGNSDLGGGGQAGGNGAPSSGGVSSGGANAGGDGAGEGGSSTEPPLG
                     GSAGETRNPQGGGAGFPDIDFNVGGSSEPPLTPDDACVQSRVHTEPIPLDVYIMLDRS
                     GSMNLPQAMPIGNTTAGGGDCNVGDTKESRWCNAINALNAFFISPDVQGTRVALQFFP
                     AGTCQSSSNPLVYGCCSSGQCCLGAAEATPDVKLGALPAQAEPLRAALNAQVPWADRT
                     PIEAALRGLLKYTSTQITPGRQVLNMLITDGGPEGCDSNQNSLARLVASYGLPTYVIG
                     TQGAAYSWLEPIAVAGGAPAHTKHCAGGVSPCHFYDVGSAKADVFVEVLKQIRRSAIA
                     CHFAVPPSDVGLVDPTRVKLSFTPANATSATTLKRVDGASACGTGGGFYYDKPLDPSF
                     ISLCPSSCDAVRAADGGDMELLVGCLGS"
     gene            2425..3543
                     /locus_tag="VFQ61_09425"
     CDS             2425..3543
                     /locus_tag="VFQ61_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9954715.1"
                     /translation="MVPELFELWPALRERLPHVALGSFPTPIQALAGVQRALEAESEE
                     TPRAPRVPALYVKRDDLSSPVYGGNKVRTLEVLMGVALARGAREIVATGAFGSNHAVA
                     TVLHAPRVALVPRVILFPQPHSFAALENARVSATHAAHFLALPHWSLLPYAMWRVGAP
                     ERYVMAPGGAVPEGALGYVAAGLELALQVRGGELPLPSRVYVGVGSTCTSAGLLVGFR
                     HAERLGIWKKAPLLVSVRVTPWPVTSRLRILRLATRAAALLNELSVGRAPGLTPQELG
                     PGLSIDGRELGAGYGRPSFAGARATELVTRVSDLRLDTTYTAKSMSGFLRAARAASDG
                     PGPFVYWATKSSAALPDPIDLAKAGLSPGFRRWLAACR"
     gene            complement(3540..4292)
                     /locus_tag="VFQ61_09430"
     CDS             complement(3540..4292)
                     /locus_tag="VFQ61_09430"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET9954716.1"
                     /translation="MESSSADRKTGTSVNIAASIFRDLRRQILRGELPSGERLPGERE
                     LAQRYSTNRNTLREAVRMLEQARLVTVRHGQGVTISDFRRTGTMDLLPPFIEQAVDLS
                     EVTHLLEDILPARLLVLEFSARLAVRRASPEDVARIKEASEQMIAAFERTDAVAIAVG
                     FQRWLEAMIDAGHSVAVRWIGNPFLDAYRELLDRFPALWVLEPTFPQHLRDLLESIEN
                     KDEERALHVMRDYYERVDRNLMQALFGLLEKR"
     gene            complement(4321..4932)
                     /locus_tag="VFQ61_09435"
     CDS             complement(4321..4932)
                     /locus_tag="VFQ61_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954717.1"
                     /translation="MPTLPGYRAARVLGLLACVGSCLAATQARADIQTPGAHPHYTLD
                     LEPHLALQFDRGWRGEGTGIGPGFRVSLPVVQNGPLKNLNNNLAVGLGLDYTHFRGCD
                     RPGTSGECSVNQYWFPVLAQWNFFFTDHWSAFAELGFSVLHRTIAWDTECPFLDAGQC
                     HSKSFRPLEPVIFVGGRYLITGRFGVVARVGSPYASLGVDFLL"
     gene            complement(4972..5661)
                     /locus_tag="VFQ61_09440"
     CDS             complement(4972..5661)
                     /locus_tag="VFQ61_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954718.1"
                     /translation="MNFFGHAAIASGTGGSPAFILGAMLPDFLAMLRLRTTPRDAHQG
                     ESELGRGMAFHHVTDHVFHDSPAFQALSRDAFAALLEAGLARGPSRALAHIGIELLLD
                     ETPEMKALGASGYLAALAYGRESGVRQLVSESGLGEAGASLEALLDLLATRGVRAGKP
                     TLPEVCERLRRALANRPRLAFSPRDDAQICAWLAHARSIVATHAPLVLSEVWAGLAAR
                     GYARTQTLSRT"
     gene            5841..6530
                     /locus_tag="VFQ61_09445"
     CDS             5841..6530
                     /locus_tag="VFQ61_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9954719.1"
                     /translation="MTRVPAELVSVRGLSNHAACLRLRVTSNRFVWRPGQHVEVSTTD
                     EPDHAVPYSIASRPLPDHPGEFELAISNRGTDWLDRLNGKTGLLVSEPQGQFIWRPEP
                     EATLLVGIGTAVAPLRAMLHAALALRADVPITLLLGARTETDILFREELWGLSRKHPR
                     FQFEPSLTHPSAHWSGLRGRIQQHLLRILHDFTPNVAYVCGSSEMVRDVLSRLEASSV
                     LTPSQIRYEMG"
     gene            6946..7653
                     /locus_tag="VFQ61_09450"
     CDS             6946..7653
                     /locus_tag="VFQ61_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9954720.1"
                     /translation="MQIAVISDLHLGPGGSVDGFGHSDTQFLKFLDFLEGNFERIVLL
                     GDVWETLTGLMPGDPAAELRAAQAAHPELARRFDRERYVYVHGNHDLVAGAVCGAPDE
                     FILQADGQRFLFTHGHHSDTWMRRGRWISELGVWLGGWIRRLGLAAVYRLFASWDQRL
                     GGASADASKCRFQRWAIDMAVSRQVDVVVTGHTHVATRAEHGSHLFLNSGTCSEGQLS
                     FLSLDTRRGQYAVNVGY"
     gene            7832..8200
                     /locus_tag="VFQ61_09455"
     CDS             7832..8200
                     /locus_tag="VFQ61_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET9954721.1"
                     /translation="MTEQIRVDRWLCAARIYKSRTQAHDACEGGLVKVNGSAVRASHA
                     VRPGDQITAQAPRGVVVLEVQKLAPKRLGPAPARELYEDHSPPPPPKEERVAPRARGA
                     GRPTKADRRAIERLRARGPE"
     gene            complement(8266..9843)
                     /locus_tag="VFQ61_09460"
     CDS             complement(8266..9843)
                     /locus_tag="VFQ61_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9954722.1"
                     /translation="MQETTLAKPLDRARGALQKLPMRALLPPLILDSTQGPLQQQVCE
                     SLRQAILAGWFAPGGRVPSSRALADSLGVSRTTALAALDQLRHQGYLASRRGSGTFVA
                     RELPDEVSLSEPRVHRADTHPVLSARGRELLTLLPPARRLGASPRPFRLGTPALDLVP
                     VQTWAKLAQRRIRAIKSSWLDYADPAGLRELREAIAALVRRRGTTCDPDSVFIVGGAQ
                     RGLEFITQLLLDPGDSVWMEEPGYPGAVHAFRVAGARPCFVPVDEDGLNVEAGRKASP
                     AARLAYVSPSHQFPLGVPMSLPRRYELLRWAQAAESWIVEDDYDCEFRYRRAPIHCLH
                     GLDPDGRVIYVGTFSKTLFPALRLGFLILPQSIHRSFVAARTTADVHSSSIDQLVLAS
                     FITEGHYERHLRRVQAAYRERLEALTEAAERFCGGVLRLRTVQSGLHAVADLYDVPAE
                     TVFQEACARGVEVAPLAQYYLTRAQPANALLLGFGPCHPEQFAPGMVQLARAIEAARR
                     GAHTERADIQALYGRLR"
     gene            9907..11157
                     /locus_tag="VFQ61_09465"
     CDS             9907..11157
                     /locus_tag="VFQ61_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9954723.1"
                     /translation="MHIKRWLFGFALISALGTWHAAQVAPVQAKSDPPHWVGTFSAGL
                     VAWDQPLDGSNQGFQNRTLRQILHVSIGGSWLRLRLSNAHGAKPLVVGSTHLALQKSG
                     ASIVPGSDRALTFGGQATITIPVGAWVVSDPIRLDVAPLSNVAVSLYVPNDTGPATWQ
                     LPSLQTSYVSPAGDFTGTPELPVEKTDPGRYWVSGLEVLAPPVVSAIVAVGDSLTHGF
                     GSTPDTNQRWPDLLSARLNARPWRLNRGVLNQGIGANRLLHEFSGNSAAARFDRDVIA
                     QSGVSHVILSEGVVDLGLPGVTGATEEQVSAAQMIQGFRQLIEKGRSRGLKMLGATLT
                     PFEGVAFPGFHSAEIEAKRQAINQWIRNGGAFDGVLDFDAVVRDPSAPTRLLPAYDSG
                     DHIHLNNAGYTAEANSIDLGLFAP"
     gene            complement(11175..>11613)
                     /locus_tag="VFQ61_09470"
     CDS             complement(11175..>11613)
                     /locus_tag="VFQ61_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954724.1"
                     /translation="APARGSSPEGGRITPTRAERETASDGIPIAPAGSTAIPSAEPHH
                     PHPITPQHERIYAENNLVASLAGALDVRDTARIRRLVAEYRERYPEDDHQIQAGYEII
                     ANCLDHPGPDTKAAAQRYYDEELGSNIRRYVRRHCLIECGRTA"
BASE COUNT         2049 a         3700 c         3766 g         2098 t
ORIGIN      
        1 acgccctcat acgatctgta gcggttcgcg atcggccgca gcgatcagga cctcgatctc
       61 gccccgggtc gcgtccagca aacgctgccg gtagcggggt aagtagcctc gttcggtatt
      121 ggagtgctca cacaaaatca cgctctgccc ctgggcaagc ttggctaaca cggcgtgatg
      181 gccgagctcg ccggtgacga atagatcggc ggtggcccgc tcgaacagcg atcctccgga
      241 acccgcacag aacgcgattc gccgaatgct cgcgccatgg tcgtgggcag cgctggccgc
      301 aagccgcacc tgagtcagcc ccagatgctg cttgagtcgc tccaagatcg taccgagtgg
      361 agtcggttgg tccagctcta gcaatcgccc cgcgccgatc gcgggggatg gctcatggcc
      421 cggggggctg atcgcggtcg agcctccctc gttggtaagc gctgggacca gcgccgtgag
      481 tcgacccgag cctgcgcctt cggcgagcca atcggtgagc cccgaaggcg ccgcatcgag
      541 cgccgtgtgc ggcgagtaca cggcgaagcc agcgtgcgcc gccgcgaaga ccacgggatc
      601 ttctgcgcgc gagaagcgct ttttcgcgcg aaacaacggc gggtgatagc tcagcaggca
      661 ttgcgcgcgg agcgactcgg cctcgctcac caccgtcggc gtggcgtcga tcgtgagcag
      721 taagcgcgag atgggaagtt cacggccgct ctcgcgtgga tcgatcaaca acccgacgtt
      781 gtcccagtcc tccgccaacc gcaggggcgc cagctggtcc aagacttgaa gcaactcggt
      841 gagaggtagc ggcatcgcgc gcgcagcatg ccatgagtgc cgcggcttcg caccggggtc
      901 ggccgcccct caggagccca aacagcctac cagcaactcc atgtctcctc catcggcggc
      961 ccgcacggca tcgcagctgc tcgggcacaa ggagatgaag ctcggatcga ggggcttgtc
     1021 gtaatagaac ccgcccccgg tgccgcaggc ggaggcgccg tccacgcgct tcagcgtcgt
     1081 cgcgctggtc gcgttggcgg gtgtaaacga gagtttgacg cgagtcggat cgaccaaccc
     1141 tacgtcgctg ggcggaactg cgaagtgaca cgcaatcgcc gaacgccgga tttgcttcaa
     1201 tacctccacg aacacgtccg ccttggcgct gcccacgtcg tagaagtgac aggggctgac
     1261 gccgcccgcg cagtgtttgg tgtgggcggg cgcgccgccg gccaccgcga taggctcgag
     1321 ccagctatag gccgcgccct gagtcccaat cacgtaggtg ggaagtccgt aagatgcaac
     1381 caaacgggcc agcgagttct ggttcgagtc acaaccttcc gggccaccat ccgtgatcag
     1441 catattcaaa acctgccgtc cgggagttat ctgggtcgat gtgtacttga gcagcccgcg
     1501 cagtgcagct tcgatcggcg tgcggtcagc ccagggcact tgtgcattca gcgcggcacg
     1561 caagggctcg gcttgggccg gcagcgctcc gagcttcacg tccggcgtcg cctcggccgc
     1621 acccaagcag cattgcccgg agctgcagca gccatagacc aagggattgg aggagctctg
     1681 acaagtgccc gccggaaaga actgcagcgc cacgcgcgtc ccttggacgt ccggtgaaat
     1741 gaagaacgca ttgagtgcgt tgatggcgtt gcaccaacga gactccttgg tgtcgccgac
     1801 gttgcagtcg ccgcctcccg ccgtcgtatt gccgatgggc atggcctgcg gcaggttcat
     1861 gctccccgag cgatcgagca tgatgtaaac gtcgagtggg atgggctccg tgtgaacacg
     1921 actctgcacg caagcgtcgt ccggcgtgag gggaggttcg gagctcccgc ccacgttgaa
     1981 gtcgatatct ggaaaacccg cgccgccgcc ttgcgggttc ctggtttcac cggcggaccc
     2041 gcctagcggt ggctcggtcg agctaccacc ttcgcccgca ccgtctccac ccgcgttggc
     2101 tcccccggag ctgactcctc cagacgaggg agctccgttc ccgcccgctt gtccgccacc
     2161 gccgagatcg gagttgccag cgtcgtgggc tccggagccg cccgcggagg acgtccccgc
     2221 actcccgccc gacggcaagc tcggcagcgg cgctgagtca tccttcgagc acgaccccgc
     2281 gagcaccgtg cagcacgccg cgattaggct gaatcccagg taacgccggg ggggcgactt
     2341 ttgcatcacg tcagagcgaa ggagcggctc gccgactggt aacttaagcg cacccagcct
     2401 gaccggtgcg ataagctcgg agtcatggtc cctgagctgt tcgagctttg gcctgctctt
     2461 agggagcgcc tccctcatgt ggctttgggt agctttccga ctccgatcca agcgctggcc
     2521 ggggtgcagc gcgctttgga ggcagagtct gaagaaacgc cgcgcgcgcc gcgcgtgccc
     2581 gcgctctatg tcaaacggga cgatctttcc agccccgtgt atggcggcaa caaagtgcgc
     2641 acgctcgagg tattgatggg ggtggcgcta gcgcgcggcg cgcgcgagat cgtagcgacc
     2701 ggtgcatttg gctcgaacca cgcggtcgcg accgtgctcc atgcgccgcg cgtggcgctg
     2761 gttccacgcg tgatcctgtt tccccagcct cactcgttcg ccgcgctcga gaatgcacgc
     2821 gtgagcgcca cccacgctgc tcactttctc gcgcttcctc actggtcgct cctgccgtac
     2881 gccatgtggc gcgtcggtgc gccggagcgc tacgtgatgg cacccggcgg agcggttccg
     2941 gaaggagcac tcggttatgt cgcggctggt ttggagctgg cgctccaggt tcgcggcggt
     3001 gagctgccct tgccttcgcg agtgtatgtg ggggtcggct cgacctgtac gagtgccgga
     3061 ctgctcgtag gctttcgcca cgcggaacgg ctggggatct ggaagaaagc accattgctc
     3121 gtgtcggtgc gggtcacgcc atggccggtc accagccggc tgcgcattct gcgtcttgcg
     3181 acgagggcag ccgctctgct caatgagttg agcgttgggc gagccccggg tttgactccc
     3241 caggagctgg ggcccggcct ttcaatagac ggacgggaac tcggagccgg ctatggccgc
     3301 ccgagttttg ccggcgcacg cgccaccgag ctcgttactc gagtcagcga tttgcggctc
     3361 gacacgacct acactgcgaa gtccatgtcc ggatttctac gcgccgcgcg agccgcctcg
     3421 gatggcccgg gccctttcgt gtactgggcc accaaatcga gtgcggctct ccccgatccc
     3481 atcgatctcg cgaaagccgg gctgagcccc ggctttcgac gttggctcgc ggcgtgccgc
     3541 taacgcttct cgagcaggcc gaatagcgcc tgcatcaggt tgcggtcgac ccgctcgtaa
     3601 tagtcgcgca tcacgtgcag agcgcgttct tcgtctttgt tttcgatgga ctcgagcaaa
     3661 tcccgcaagt gctgcggaaa ggtgggctct agaacccata gcgccgggaa gcgatcgagg
     3721 agttcgcggt atgcgtccag aaacggattt ccgatccagc gcacggccac cgagtgcccc
     3781 gcgtcaatca tggcctcgag ccaacgctga aaacctaccg caatcgccac cgcgtccgtc
     3841 cgctcgaagg ctgcgatcat ctgctcgctg gcctctttga tgcgggccac gtcttcgggg
     3901 ctcgccctcc gcaccgccaa gcgtgccgag aactcgagta cgagcaagcg cgcgggcagg
     3961 atatcttcga gcaggtgtgt cacctcgctc aaatccaccg cctgttcgat gaacggcggc
     4021 aacaagtcca tcgtgccggt gcgccggaaa tcggagatgg tgacgccttg cccgtgtcgc
     4081 acggtgacca ggcgcgcttg ctcgagcatt cgaaccgctt cgcggagcgt gttgcggttg
     4141 gtcgagtagc gctgggcgag ctcgcgctca cccggcaggc gctctcccga gggcagctct
     4201 cctctcagta tttgccgtct gagatctctg aaaatcgagg cggcaatgtt gaccgacgtg
     4261 ccggtcttgc gatcggccga cgaggactcc atgaagctga ccctttcgcg aggcagcgct
     4321 tcatagcaag aaatccacgc cgagcgaagc gtaggggctt ccaacccgag caactacccc
     4381 aaacctgccg gtgatcaggt aacgcccgcc gacgaagatc accggttcca ggggccggaa
     4441 gcttttcgag tggcattgcc ccgcgtcgag gaaggggcac tcggtgtccc acgcgatcgt
     4501 ccgatgcagt acggaaaagc caagctccgc gaaagcgctc caatggtcgg taaagaagaa
     4561 gttccactgc gcgagcacgg gaaaccaata ctggttcacg ctgcactcgc cggacgtacc
     4621 tggtcgatcg cagcctcgga aatgcgtgta atcaaggcct aggccgacgg ccaggttgtt
     4681 gttcaggttc ttgagcgggc cgttctgcac taccggcagc gagacccgaa aaccgggacc
     4741 gatccccgtg ccttcgccgc gccagcctcg gtcgaactgc aaagcgaggt gtggctccag
     4801 gtccagggtg tagtgcgggt gggctccggg cgtttggatg tcggcgcgag cctgggttgc
     4861 ggcgaggcag ctgcccacgc atgcgagcag ccctagaacc cgtgccgcgc gataacctgg
     4921 gagggtaggc attgtttcct aagagtgact tatcggggcc tatccgggtg cctaggttcg
     4981 agacaacgtc tgggtgcgcg cgtagccgcg agccgcgagc cccgcccaga cctcgctcag
     5041 tacgagcggc gcgtgtgtag ctacgatcga acgcgcgtgc gcgagccatg cgcaaatctg
     5101 ggcatcgtcc cgcggggaga acgcgagtcg agggcgattg gcgagggctc gccgcagccg
     5161 ctcgcagacc tcgggcagcg tcggctttcc cgcgcgtacg ccccgtgtcg ccaacaaatc
     5221 caagagcgcc tcgagcgagg cgccagcttc acccaagccg ctctcggaga cgagctgccg
     5281 cacaccgctc tcacggccgt acgcgagcgc agctaggtac ccgctggccc caagtgcctt
     5341 catttcgggg gtctcgtcca ggagcaactc gatcccgata tgggccagag cgcggctcgg
     5401 gcctcgcgcc aagcccgcct cgagcagagc ggcgaaagca tcgcgggata acgcctgaaa
     5461 agccggtgag tcgtggaaaa catggtcggt cacgtgatga aaagccatgc cacggcccaa
     5521 ttcgctctcg ccctgatgcg catccctggg ggttgtccgc aggcggagca tcgccaagaa
     5581 gtccggcagc atcgcaccga ggataaatgc gggcgatcca cccgttccgc tcgcgatcgc
     5641 ggcatgccca aagaagttca caagcgcgag tttagagctt ttccgggtag gttcatccca
     5701 gcgcaccgga gatattgccc gcggtccgct cgaccacggc tttgtttgga aaatgcggcc
     5761 ggctggcgga atctgagtta tctcacaggg tgacgcttgc tgcgtgcgcg tcttgaccca
     5821 aagtcaccat actcccggcg atgacgcgcg ttcccgcaga attggtttcc gttcgaggcc
     5881 tctccaatca cgccgcgtgc ttgcgattgc gggtcaccag caaccgattc gtttggcgcc
     5941 cggggcagca tgtcgaagtc tcgaccacgg atgagccgga tcacgcagtt ccatactcga
     6001 ttgcctctcg tcctctaccg gaccatcccg gcgagttcga actcgcgatc tcgaatcgcg
     6061 gaacggactg gttagaccgg ttgaatggca agaccggcct gctcgtctcc gaaccgcagg
     6121 ggcaattcat ctggcgtccg gagcccgaag cgacgttgct cgtcgggatc ggaacggcgg
     6181 ttgcacctct ccgcgccatg ctgcacgcgg cactggcgct gcgggcagat gttccaatta
     6241 ccttgttgct cggtgcccgc acggaaaccg acattctgtt tcgagaagag ctttggggcc
     6301 tgtcccgcaa acatccccgt tttcagttcg agcccagtct cactcatcca agcgcgcatt
     6361 ggagcgggtt gcgtgggcgg atccaacaac acctgttgcg gattcttcac gacttcacgc
     6421 ccaacgtcgc ctacgtctgt ggcagttccg agatggttcg agatgtgctt tctcggctcg
     6481 aggcgagctc ggttttgacg cccagtcaga tccgctacga gatgggttga ctgacgtccg
     6541 gacacattcc ggtcacgtgt ccgcgaccgc gctggattga caatcggggg taatggtccg
     6601 acgtacaatg ggcgtatgca tgctttggaa gcgaccgagc ggtagagcga gccgagcgta
     6661 gtatcgagac cgcgaagcgc ccggtccctt cgagtagata ccaaagattg gattaaccac
     6721 atccaatcca agcatgaatt ctggtgcagt ggcgccccgt attgtcgggt tcggtgttcc
     6781 cggttcgggg ccgcgccttc gggctaggcc gagttcaaag gtcgaatgct cgaagacgat
     6841 acgccgtggt ttcgagcttc ttgctggccc tccacgctgg cgtcaccgag ctcccgcaat
     6901 gtccgcccaa aaaacagatt gtccccaaag ctagcgagga cgcacatgca gattgcagtc
     6961 atatcagacc ttcatctcgg accgggcgga tctgtggatg ggtttggcca ctcggatact
     7021 cagttcctca agttcctcga cttcctcgaa gggaacttcg agcgtatcgt tttgctcggt
     7081 gacgtttggg agaccctgac ggggttgatg ccgggagacc cggcggcgga actgcgcgcg
     7141 gcgcaggccg ctcatcctga acttgcgcgc cgcttcgatc gtgagcgtta cgtgtatgtg
     7201 cacggcaacc acgacttggt cgcaggggcg gtctgcggcg ccccagatga gttcatcttg
     7261 caggccgacg ggcagaggtt cctcttcacg catggccacc acagcgacac ctggatgcga
     7321 cgagggcgtt ggatctcgga actcggggtg tggcttggcg gctggatccg tcgtttgggg
     7381 ctggcggccg tttatcggtt gtttgcctct tgggaccagc gtttgggcgg ggcgtccgcc
     7441 gatgcgtcga agtgcaggtt ccagcgctgg gctatcgata tggcggtgag ccgacaggtt
     7501 gacgtcgtgg tcacggggca cacgcatgtg gccacccgcg ccgaacacgg ctcccacctc
     7561 ttcctgaaca gcgggacctg cagcgaaggc cagctgagct tcttgagctt ggatactcgg
     7621 cgcggccaat atgcggtgaa cgtgggctac tgagcccgga gttcgcgcct tctggtccga
     7681 ccccgataac tcctcgcgat ctcttctccg ctcggctgcc cccgtggcgg gcgattgggc
     7741 tgagccgacc cggcgcgggt ggggcgaggt tggagcgtac atgcgctcga atttcggttg
     7801 aaacttgcga gtgctgtgtg acgatcgacg catgaccgag cagatccgcg tcgatcgttg
     7861 gctgtgcgcg gcccgcatct acaagtcccg cacccaggcc cacgacgcat gtgaaggcgg
     7921 cctcgtcaaa gtcaacggtt cggccgtgcg cgccagccac gcggttcgcc cgggtgatca
     7981 aatcacggct caggcccccc gaggagtggt ggttctagag gttcagaagc tcgcgccgaa
     8041 acgcctcggc cccgcgcctg cacgcgagct ttacgaggat cactcgccac caccgccgcc
     8101 caaagaggag cgcgttgctc cgcgcgcccg aggcgcgggc cgccccacga aagcagatcg
     8161 gcgtgcgatc gagcgcctac gtgctcgcgg tcccgaatag cacctgcgct cgggccgaga
     8221 gagcggcagt gcccggtgga gatttctaag cgagccgcag agctcctagc gaagccgacc
     8281 atagagcgcc tgtatgtctg cgcgttcggt gtgggcgccg cgccgggccg cttcgatcgc
     8341 gcgcgcgagt tgaaccatgc cgggggcgaa ctgttcaggg tgacaaggcc caaaacccag
     8401 gagcaaggca ttcgcaggtt gagctcgtgt caggtagtac tgggcgagcg gcgccacttc
     8461 cacgccgcgc gcgcaggctt cctgaaacac ggtctcggcc gggacatcgt acaaatcggc
     8521 gaccgcgtgc aacccgctct gcacggttct cagccgcagc acgcccccgc agaagcgctc
     8581 cgcggcctct gtcagggcct ccagtcgctc gcgatacgct gcttggacgc gccgtaaatg
     8641 ccgctcgtag tgcccctccg tgatgaaact cgcgagcacc agctgatcga tgctggagga
     8701 gtggacatcg gccgtagtcc gagccgcgac gaaggagcgg tgaatgctct gcggcaaaat
     8761 cagaaaaccg agccggagcg ccggaaatag ggtcttactg aaggtgccga cgtaaatcac
     8821 tcgaccgtcc ggatcgagcc cgtgcagaca atgaatcggt gcacggcgat accgaaactc
     8881 gcaatcgtag tcgtcctcca caatccaaga ctcggcggct tgggcccagc gcagtagctc
     8941 atatcgacgg ggtaagctca tcggtacgcc gagcgggaac tggtgcgatg gtgaaacgta
     9001 ggccaatcgc gcagccgggc tggctttgcg gcccgcctct acattcagcc catcctcatc
     9061 caccggcacg aagcagggcc gtgcacccgc cactcgaaag gcatgaacgg cgcccggata
     9121 gccaggctct tccatccata ccgagtcacc cgggtccagc aacagctgag tgatgaactc
     9181 gagtccccgt tgtgcgccgc cgacgatgaa caccgaatcg ggatcacacg tcgtgccgcg
     9241 acgacgcacg agggccgcga tggcctctcg gagctctcgc aagcccgccg gatcggcgta
     9301 atccagccac gaggatttga tggctcgaat tcgtcgctgg gcgagcttcg cccaggtttg
     9361 cacggggacg agatccaagg cgggcgtgcc caatctgaat gggcggggac tcgcgccgag
     9421 ccgccgcgcg gggggcagca gggtgagcag ctcgcggccg cgcgcggaca acaccgggtg
     9481 ggtgtctgcg cggtgcacgc gcggctcact caggctcact tcgtctggta gctcgcgggc
     9541 cacgaaggtg cccgagccgc gtcgtgaggc caaatagcct tgatggcgca gctgatccaa
     9601 ggcggcgagc gcggtggtgc gagacacgcc gagcgagtcc gccaatgctc gagaagaggg
     9661 cactcggcca cccggggcaa accagcccgc caagatcgcc tgacggaggc tttcgcagac
     9721 ctgctgttga agcgggcctt gagtcgaatc caggatgagg ggaggcagca acgcccgcat
     9781 cggcagtttt tggagggctc cgcgagcgcg gtcaaggggc ttggccaaag tggtctcctg
     9841 catcggagtg gaagtggatc tgtccgtgat tgccgctcgc gacatagaca agcggaggca
     9901 gaatacatgc acatcaaacg ttggctattc gggtttgcgc tcatttcggc cctaggcacg
     9961 tggcacgcgg cgcaggtcgc accggttcaa gccaagagcg atccacccca ctgggtaggc
    10021 acattcagcg ccgggctagt ggcctgggat cagccgttgg acggcagcaa tcaaggtttc
    10081 caaaaccgta ctctgcggca gattttgcac gtgagtattg gtggctcttg gctgagactg
    10141 cggctgtcca atgcccatgg cgccaagcca ctcgtggtgg gtagcacgca cctggcgctg
    10201 caaaagagtg gggcttcgat tgtcccgggc tcggaccgtg cgctgacctt cggcgggcaa
    10261 gcgaccatca ccattcccgt tggcgcctgg gtggtgagcg atccgatacg gctcgacgtg
    10321 gcgccgctct ccaacgtcgc ggtgagcctc tatgtcccga acgacaccgg gcccgcgact
    10381 tggcagctgc cgagcttgca aaccagttat gtgtcacccg cgggcgattt cacggggacc
    10441 cctgagttgc ccgtcgagaa aaccgatccg gggcgttatt gggtttctgg gctggaggta
    10501 ctggcgccac ccgtggttag tgcgatcgtc gccgtcggag actcgctcac tcacggtttt
    10561 ggctctaccc cggatacgaa ccagcgctgg cccgacctgc tctccgcgcg cctcaatgct
    10621 cggccctggc gactcaatcg cggggtgctc aatcagggga tcggcgcgaa ccggctactt
    10681 cacgagttct ccggaaacag cgccgccgcg cgattcgacc gcgacgtgat cgcgcaatcg
    10741 ggggtaagcc acgtgatctt atcggagggc gtggtggacc ttggcctgcc cggtgttacc
    10801 ggcgcgaccg aggagcaggt gagtgcggct caaatgatcc aaggctttcg ccagctgatt
    10861 gagaagggcc gttcgcgcgg cttgaagatg ctgggcgcga ccctgacccc gttcgaaggc
    10921 gtggcattcc cgggctttca tagcgcggag atcgaggcca agcgtcaggc catcaatcag
    10981 tggatccgca acggcggagc cttcgacggc gtattggact tcgacgccgt cgtccgcgat
    11041 ccgagcgcgc ccacacgctt gctccccgcg tatgacagtg gagaccacat ccacctgaat
    11101 aacgccggct acaccgcgga ggccaactcc atcgatctag ggttgttcgc accgtaacct
    11161 agcacgcagc gcaatcaggc agtgcggccg cactcaatca ggcagtgcct ccgtacgtag
    11221 cgtcgaatgt tcgaacccag ctcttcatcg taatagcgtt gcgccgccgc cttcgtgtcg
    11281 ggtccagggt gatccaagca gttggcgatg atctcgtatc cagcctgaat ctggtggtcg
    11341 tcttccggat agcgctctcg gtactcggcg accaggcggc gaattctagc cgtgtcgcgc
    11401 acgtcgagtg cgcccgcgag cgaagcaacg aggttgttct ctgcgtaaat gcgctcgtgc
    11461 tggggcgtga tcgggtgtgg atggtgcggc tcggctgacg ggattgccgt gctccctgcg
    11521 ggagcgatgg ggatcccgtc actcgcggtc tcacgctcgg cccgcgttgg cgttatccgc
    11581 ccgccctccg gcgagcttcc ccttgctggc gct
//
LOCUS       DASTKU010000263        18632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_719785, whole genome shotgun sequence.
ACCESSION   DASTKU010000263 DASTKU010000000
VERSION     DASTKU010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 18632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18632
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719785"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2344)
                     /gene="topA"
                     /locus_tag="VFQ61_09475"
     CDS             complement(<1..2344)
                     /gene="topA"
                     /locus_tag="VFQ61_09475"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET9954725.1"
                     /translation="MGKSLVIVESPAKARTISGFLGNKFVVESSIGHIRDLPKRAEDI
                     PAVYKKAPWARLGVDVEHDFKPLYVVDPEKRAHIQKLKQLVKQADELFLATDEDREGE
                     SIAWHLLEVLEPSVPVKRMVFHEITEKAISEAIRSPRQIDRRLVDAQEARRILDRLYG
                     YEVSPVLWKKVMPKLSAGRVQSVATRILVERELQRMRFRAAVYFDIEGKFQVAPEARR
                     PGVPAELAADLVTVDGQRVASSKDFKDDGTLVRSDVLLLDEARANALAKELEAARVSV
                     TSVERKPGRRSPAAPFMTSTLQQEASRKLRFASARTMRAAQSLYERGYITYMRTDSTT
                     LSEVAISAARAQIQQLYGAEYLPPKPRLYTKKVKNAQEAHEAIRPAGEAFRLPSEVHG
                     ELGSDEAKLYELIWTRTVASQMQDAQIESAKIMLLAATSQRRAELSVTGNTIVFPGFL
                     RAYVEGSDDPSAELENRERPLPNLKEGEQLESLGYTPRGHQTQPPARFTEASLVRRLE
                     ELGVGRPSTYASIMDTIQGRGYVWKKGTALVPSFKAFAVTRLLEQHFGNLVDYEFTAR
                     MEDELDGIANGQTESVPWLKRFYFGGSDAATLGLQKLVGERLGEIDARQINSLPIGKD
                     VEGREIVVRVGRYGPYLQRGEQTVTLPEDIAPDEITVERASQLLEAPSGDRQLGSDPE
                     SGLPVYVRAGRFGAYVQLGEAGEKNKPRTASLMRSMSAQTLTLEQALQLLSLPRVVGN
                     DPADNVPITAQLGRYGPYISKGTDSRSLENEEQVFTIDLEA"
     gene            complement(2555..3430)
                     /locus_tag="VFQ61_09480"
     CDS             complement(2555..3430)
                     /locus_tag="VFQ61_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954726.1"
                     /translation="MKQIASALALTSLLASCYRDAPPSRFPSARAALDRMQESVSCSR
                     GLSGEAKIDYFGAEGRVRGSVLYLVEAPDHVRLDLFSPFGVTLSTLTANGSRFTLSDL
                     RKRRFLYGPANSCNLKRFTRVPLPPQAFAQLLRGEAPILKHSAPAASISWEKGSYAIR
                     IASAHQTEELIRLVPVDADYGLDWSKQRLHVLEVSVSQAGVPIWQVELADHAPVKMSK
                     PQLDPEGLEPPVPPSGPECRAAAPRRLRFVVGDAESEVVLVSHEMAHNPPLSLGVFEQ
                     QQPPGFAAEASLCRD"
     gene            complement(3427..5250)
                     /locus_tag="VFQ61_09485"
     CDS             complement(3427..5250)
                     /locus_tag="VFQ61_09485"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HET9954727.1"
                     /translation="MTFTEAAAQVLRLVGKPLHYKEITDVAIEKNLLSHVGKSPEVTM
                     GARLAALVKKGDKENPLVRVKPGVFALREWDQETIDKGLRDRTPALEKLSGQDIAFET
                     EQEASGASHVSLDTMNEEGETLSPDDEERERAELAAHATDLFESEEDDDRPIFGEEAE
                     PAADDAQRVADVNGRRRRRRRRRGRGGDDRGDADELPSYTVSDAPAELTEALLEVERE
                     PAREAREPREAREPREAREPREGREAREPREAREPREGREPREAREPREGREPREARE
                     PREGREPREAREPREGREPREAREPRETRAAREGREPREERDRDREVRDRELREPRSE
                     REGDVRAPLNDLSGKPLADGIETLLGTFERGRGPVSMQSLIDSAQRRGRLTGEPAVAQ
                     ALVQTAVRADNARRAGEGRRPRFRLAGNRVALADWLVDPELSRAERDLQNALERYREA
                     SRRSLLRALQELPQRALGELVVLLLEQMGYTELVAVRRPGSHGAELHLSGKARSLFGE
                     ARTAIVVRRDGREIGRERVTELRGALHHYGPAVAGLLITTGQALSGAREEAAAHAAAP
                     VTVLDGVLLARACEEHCVATSALQLKLAVPDTELLDGLKVG"
     gene            5547..5972
                     /locus_tag="VFQ61_09490"
     CDS             5547..5972
                     /locus_tag="VFQ61_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954728.1"
                     /translation="MAARTQGIEEPTYIGAPPGYTEPVVPAELRDELLLVARSAGLEI
                     RNVEMRRKNAGPGGLCTLNGEAVLILNQRASAIEQATVLGDALAGRDLTAVAMPSYVR
                     GFIAMRQRARSRLLVPERRPGPGVVRGNVETFRRRRGDP"
     gene            5992..7029
                     /locus_tag="VFQ61_09495"
     CDS             5992..7029
                     /locus_tag="VFQ61_09495"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET9954729.1"
                     /translation="MLIRNDVPLADYTTLKLGGPAKHFVEVRDENELAEALSWARSRN
                     ERVFVLGGGSNLIVPDAGVDGLVVSIALRGIAHDTGKAGSTLLRVQAGEPWDSVVGVA
                     LEANLAGIESLSGIPGQAGATPIQNVGAYGQEVKDTIVRVRVFDREDRCFSELTAAEC
                     EFSYRHSRFKTRDRDRFIVTRVDFELQTRDRADVRYPELARALAERGREASLVCVRQT
                     VLELRRSKSMLIDAQDPNGRSCGSFFLNPIVEPQAADRAQSALGVGEMPRYPQSDGRT
                     KLSAAFLIEHSGFAKGTRVGHVGISTRHSLALVAHAGATSAELMQFASQVQDGVRART
                     GVLLSLEPVLW"
     gene            complement(7125..9815)
                     /gene="alaS"
                     /locus_tag="VFQ61_09500"
     CDS             complement(7125..9815)
                     /gene="alaS"
                     /locus_tag="VFQ61_09500"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET9954730.1"
                     /translation="MTTPTTSSEIRQAFLEFFRGQGHEVVKSGSLIPQNDPTLMFANA
                     GMVQFKDVFTGRERRPYRRATTSQKCIRISGKHNDLEAVGPSPRHNTFFEMLGNFSFG
                     DYFKEEAIAFAWEFLTKVLCLPQDRLVMTYFGGAQGIPEDTVARDLWRKLTGFSEDRV
                     RPLGMSDNFWSMGDTGPCGPCSEIYFYNGSGKPDPARFGEEQTAQGEGYMEFWNLVFM
                     QFERSLDADGSPKLASLPAPSIDTGAGLERIACMLQGKGTAYDTDILRELVETAARLS
                     GKPYGGTSSPDDVSMRVIADHSRTTAFLIAEGVTPDRTGRDYVLRRVMRRAIRHGHRL
                     GIEKPFLHEVALRVVDVMGDHYPELRERRDLIASLAEGEEVRFRATIERGLSLLDISF
                     DELRKKGSKELPGAEAFKLYDTFGFPLDLTEVICTEQGFTVDQDGYKAALEKAREQSD
                     FKGVEQAVEGVYREALALVPGAAVRFTGYDKDRDEARIVAMMRDHKMVDVAHTGDEVE
                     IVTELTPFYGEAGGQVGDQGVITTVAAQIQIEETSRPISGLFVHRGKVTQGTVAVGER
                     AELKVDIERRERTRRNHSATHLLHWALRKVVGSHAQQKGSLVGPDRLRFDFAHGKPLT
                     PEEISRIEELVNERTLQNHPIRTEVLTMDEARTRGAMMIFEEKYGDTVRLLSMGESLE
                     LCGGTHARATGDIGLFKIVSEQGVAAGVRRIIGVTGAGALAYVREVESSLSRTAALLK
                     TSPNTLSERVEKLLQRERNLEKQIEDLTRKLSTGGAGGFDAWLAQARDVSGVKVLGLR
                     SEVTDRAALRELAEKLRDKLGESVVLVGSVSEGKAQLVLTVSRALSSRVKAGELIRPI
                     AQIVGGSGGGRPDMAQAGGTDVARLDEAIEALYQHVPAAN"
     gene            9866..12412
                     /locus_tag="VFQ61_09505"
     CDS             9866..12412
                     /locus_tag="VFQ61_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954731.1"
                     /translation="MGKVEGLPPPAATAPPELAAFMAERGIGPLERFVRLSGRLKLPE
                     VSDQELSGWTLCLSQRGLWLVQSSGRFSGHVLELTNPDFESARRLRYESGRLRDRLVI
                     DGQPLRVPPNAGRRARQLIGVGRIRAQRDATEPGGRGALIEGTIEPPQQLELGQVGPL
                     ARLFLNGLARPDEPWLVIIELGESHLLSALQTKARAGCYLALSPERIVYARVSDLGDC
                     ECTPLDPSALELDTSNGDAQLRAGQRLIPVRSAATRDIAEWLGLGGLERAERLYEAAR
                     RWHLKLPPNQRSQLGAASRSGWLDLAFRAGSVRAGLVLVWLDRAGRFAPGGEAAARLA
                     LVASSLAGSEGSRDEEGKSYSDLGARWEVPSDEARRWLHDLERLSAPLPRLALELHRA
                     EAKRWLEVTAAAPPPVHVEIELAEHELRAGFPEHARALAGVRLARLEDDESALFEAGL
                     EQLERARWFELLAREAEQRNESDVRALSALARLQPQESARLERLARAHSPDPREGRII
                     ERARQVLVCLEPGGLASPAVIAGDRLVLPLSTTERERLRHPLARGSARVSARLSELIA
                     KTAEPDVGFLRDYCEVLSDERHPEASRALRRAVTFLSLPQLKAYISHGARSLGLRGFS
                     ARPPFVLIGASHLDPNNPARLHDREFDFAFAAELAHLAFDQKRVTTSEVLSGAAGKTR
                     EALSLLGLALPLIKEVSGGRAQKVLDRVSKEALDRAAEGAARLPELFGSRTSGEGPTS
                     TLARANEELIAAHRLVQLTADRSGLVCANSLHAALRCMLLTRSDYHTILATTAQAGLR
                     KALFESGSQDPAAQDLAFRVRALIAFYLSDDFDTLTASDSGSAESVVDPG"
     gene            complement(12337..12858)
                     /locus_tag="VFQ61_09510"
     CDS             complement(12337..12858)
                     /locus_tag="VFQ61_09510"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HET9954732.1"
                     /translation="MSEETPLHVTEAIVWTDGAARGNPGPAGAGAVIKTRDGNILAEV
                     TEYLGTTTNNVAEYKALLLGLERALELGVRRLEVRADSELLIKQLRGEYRVRHYGLKP
                     LYEAAKQLLGKFERFELNHVRRELNSEADRLANRGIDGSTSAVEADAQPGSTTDSAEP
                     ESDAVSVSKSSDR"
     gene            complement(12979..14091)
                     /locus_tag="VFQ61_09515"
     CDS             complement(12979..14091)
                     /locus_tag="VFQ61_09515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9954733.1"
                     /translation="MAPPFHDGAKCLVRDVATHLRAVEPCVLSTRTAPPMWSRIDGSR
                     VASVPVYSDAGAYTPLLAQNFRAALWVLLASRADVWHFVFAPNPRTSTVGRVLSRARR
                     VPVVQTIASGPRHFEDVQSLLFGDVVVAQSEWMRQRVRDSAAAARAPLPDVRVILPPV
                     SMAITRSEEQREKARAELGLNGDAPWFVYPGDLETSSGAEVSAQIAERIGAALPGAVI
                     VFAFRRKTARALEIANALRSRLPSGCARFVDSTPDVLSLITASHGVIFPVDDLYGKVD
                     LPIVLLEAMVLGVPVVTLAAGPLVELRGATQVQGLDPERWIAALQSLVSECDRAARCV
                     REQREFVRNSASAEHVAAQYESVYRELSRAVPRASD"
     gene            14289..15341
                     /locus_tag="VFQ61_09520"
     CDS             14289..15341
                     /locus_tag="VFQ61_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9954734.1"
                     /translation="MLTSCKREASAADPPEVKHGRELYGRMCSVCHGDKGEGYKADQA
                     PRLAQPEFLGSASDAFLRAAIRDGRRGTTMSAWAQSHGGPLTDKDIEDVVRFLRTWQK
                     GTRVKLDESPLTGDVSRGTQLFTQRCELCHGKQGVAGLNVRIGAADFLATASDGFLRH
                     AIKRGRPGTPMKGFESSLGKQGVDDVIAALRSFATPPPVAAPEPPKPPPPMPLGPVPM
                     NPKGPEPHGFKLYPAMTGADVIKAEYDRKSRFAILDARAPSDYLNEHIAGAVSVPFYD
                     PAPYLDKLPRDAWLVCYCACPHAESGQLAQRLLDAGFKKVTVLNEGLLYWKSKNYPLG
                     RGEAKPAGDPPKAPSP"
     gene            15391..15747
                     /locus_tag="VFQ61_09525"
     CDS             15391..15747
                     /locus_tag="VFQ61_09525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9954735.1"
                     /translation="MAFTTTASGLSYEDTKVGTGPSPGRGQTCVMHYTGWLWQDGKKG
                     AKFDSSVDRGRPFSFPLGMGRVIRGWDEGVADMKVGGQRTLLIPPDLGYGASGAGGVI
                     PPNATLLFEVELLELR"
     gene            complement(15799..16698)
                     /locus_tag="VFQ61_09530"
     CDS             complement(15799..16698)
                     /locus_tag="VFQ61_09530"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9954736.1"
                     /translation="MRLTLVTGWLCCMLLHTTLSSAAPPFTTLSDRLRGDPDLTVRAQ
                     AALALGKTRDPRALKPLILALDDHAPAVRAAAAVGLRGLGDPRGVPALRLHSADGAEN
                     VREEIKSAISVLSARAPRPQSLMRVVVQLAKVHNSTRVKSESVEQDVLNSARKRLSEV
                     PYVVVESRVGSSPADGPSSTAAPAKASRSRSGEAAKVSEDKLAENNAECVVLIVPSIQ
                     KLAAEREPDGIRYSATIEFLVHSMPDEAISARLSGSASATASFAETKDRARIAELRRQ
                     VIEAAIQSALSRAPGALLAASRF"
     gene            complement(17204..17773)
                     /locus_tag="VFQ61_09535"
     CDS             complement(17204..17773)
                     /locus_tag="VFQ61_09535"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9954737.1"
                     /translation="MKVSARASLTNPFSMCLAVYLCATLTGVVSCASGASGVGSAHAV
                     SDHPLLGSAAPPLEVGVPSGRGRVNLADYAGKVVVLDFWATWCEPCRKSFPAYQSLYE
                     QFHGELAVVAVSVDESSSGIAAFVRATGAKFPIGWDEGQSAAQSYTPPKMPTSFVIDQ
                     NGIVRFVHAGYSEGDEAQIRNEVHSLLSK"
     gene            complement(18009..>18632)
                     /locus_tag="VFQ61_09540"
     CDS             complement(18009..>18632)
                     /locus_tag="VFQ61_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HET9954738.1"
                     /translation="TGVARGVGAPNVRGMFGITFVSEKSDEDNDGIDDKADQCPAAAE
                     DRDGYEDGDGCPDGDNDLDSIPDAADKCPMQAEDADGFEDADGCPELDNDKDGLADTA
                     DQCPLQAETKNNYKDEDGCPDEADVDNDGVPDARDKCPNEAEDTDGFDDTDGCPDPDN
                     DSDTILDDQDECIDEPETLNGYQDEDGCPDEDPRGGRRPAAKPAPKK"
BASE COUNT         3218 a         6163 c         5900 g         3351 t
ORIGIN      
        1 ccgcctcgag gtcgatcgtg aagacctgct cctcgttttc caagctccgg ctatccgtac
       61 ccttgctgat gtacggacca taacgaccga gctgggccgt gatggggacg ttgtccgccg
      121 gatcgttgcc cacgacccga ggcaggctga gcagctgcag cgcctgctcc aaggtcagag
      181 tctgggccga catactgcgc atcagcgagg cagtgcgtgg cttgttcttt tcgcccgctt
      241 caccgagctg cacgtaggca ccgaagcgtc cggcgcgcac gtatacgggt agtccggact
      301 ccgggtcact gccgagttga cgatctccgc tcggggcctc gagcagctgg ctcgcgcgct
      361 ctaccgtgat ctcgtcgggc gcgatgtcct ctggcaaggt aacggtctgc tcgccgcgct
      421 ggaggtaagg cccgtagcgc cccacgcgca cgacgatctc gcggccctcg acgtctttgc
      481 cgattgggag cgagttgatt tggcgagcgt cgatttcacc gaggcgctcg cctacgagct
      541 tctgcagccc caacgttgcc gcgtcactgc cgccgaagta gaagcgcttc agccacggta
      601 cggactcggt ctgaccgttg gcgatgccgt cgagctcatc ctccatgcgc gccgtgaact
      661 cgtaatcgac gagattcccg aagtgctgct ccaacaggcg ggtcaccgcg aaggctttga
      721 aggatggcac caatgcggtg cctttcttcc agacgtaacc gcgcccctgg atggtatcca
      781 tgatcgaggc gtaggtagac ggccgcccca cgccgagctc ttccaagcga cgcacgagcg
      841 aggcttcggt gaagcgagcc ggcggctgcg tttgatgacc acgcggcgtg tagccgagcg
      901 attccagctg ctcaccttct ttgaggttgg gcagtgggcg ctctcgattc tcgagctcgg
      961 cggaaggatc atcgctgccc tccacgtacg cgcgcaagaa gccggggaac acgatggtgt
     1021 tccccgttac gctgagctct gcgcgtcgtt gggacgtcgc cgccagcaac atgatcttgg
     1081 cgctctcgat ctgagcatcc tgcatctgcg aggcaacggt acgcgtccag atcagctcgt
     1141 acagcttggc ctcgtccgag ccgagctccc catggacttc gctcggtaac cgaaacgctt
     1201 cacccgccgg gcgaatcgcc tcgtgcgcct cttgagcgtt ctttaccttc ttggtgtaga
     1261 ggcgcggttt gggcggcaag tactccgccc cgtagagctg ctggatttgg gcgcgagccg
     1321 cggagatcgc gacttcggac agcgtcgtgc tgtccgtacg catgtaggtg atgtagccgc
     1381 gctcgtagag cgattgcgcg gctcgcatgg tgcgcgccga tgcgaagcgc agcttgcgtg
     1441 atgcttcctg ctgcaaggtg gaggtcatga acggagcggc gggagaacgc cggcccggct
     1501 tgcgctctac cgaagtgacg ctgacgcgcg ccgcctcgag ctctttggcg agcgcgttgg
     1561 cacgcgcctc gtccaagagc aggacgtcac tccgaaccag ggtgccgtca tccttgaagt
     1621 ccttggaact ggcgacgcgc tgaccgtcga cggtaaccag atccgccgcc aactccgccg
     1681 ggacaccagg gcgacgagcc tcgggagcca cctggaactt gccttcgatg tcgaagtaaa
     1741 cggcggctct gaagcgcatc cgctgcaatt cacgctcgac cagaatgcgc gtggcgacgc
     1801 tctgcacgcg gcccgccgag agcttcggca tgaccttctt ccagagcacc ggcgagacct
     1861 cgtagccgta taggcgatcg aggatgcggc gcgcttcttg cgcgtccacc agtcggcggt
     1921 cgatctggcg cggagaacgg atggcctccg aaatcgcctt ctcggtgatc tcgtgaaaaa
     1981 ccatgcgttt gaccggcacg gaaggctcca gcacctccag cagatgccag gcgatcgact
     2041 ccccctcgcg atcttcgtcg gttgccagaa acagttcgtc ggcctgcttg acgagctgct
     2101 tcagcttttg gatgtgcgct ctcttctctg ggtcgaccac gtacagcggc ttgaagtcgt
     2161 gctcgacatc tacccccagg cgcgcccacg gcgccttttt atacacagcc ggaatgtcct
     2221 cggcacgctt cggcagatca cggatgtgac ctatcgagga ttcgacgacg aacttgttcc
     2281 caagaaaacc cgaaatggtt cgggcctttg cgggagactc gacgatcacc agcgactttc
     2341 ccacggaggg gccgacgtac aacccgaaca aagttctgtc aattccaacg ctcgcccggc
     2401 ggtgcttccc ggcgtctcga gcggcccctc cgcgccaaca aacccggacg tttcaagaag
     2461 ctagcggagc gccgtaaaaa aagagctgcg ttcccagaag tgttcatttt gaccaaccga
     2521 ccgaggcgct cgccccagcg ctccgtggcc tccgctagtc gcggcacaaa gatgcctcag
     2581 cggcgaagcc gggcggttgc tgctgctcga agacgcccaa agacagcgga ggattgtgag
     2641 ccatctcgtg gctcaccaaa acgacttcgc tctccgcgtc cccaaccacg aagcggaggc
     2701 ggcgcggagc cgccgcgcga cactcgggcc cgctcggggg caccggaggc tcgagcccct
     2761 ccggatcgag ttgtggcttg gacatcttca cgggcgcgtg gtccgcgagt tcgacttgcc
     2821 agatgggcac gcccgcttgc gataccgaga cctcgagtac gtggaggcgc tgcttcgacc
     2881 agtccaaccc gtaatccgca tccaccggca cgagtcggat tagctcttcg gtctggtgag
     2941 cagacgcgat gcggatcgca tagctcccct tctcccagct gatgctcgcc gccggcgccg
     3001 agtgcttgag gatcggtgcc tccccgcgca ggagctgggc aaaggcctgg ggcggaagag
     3061 ggacgcgcgt aaatcgcttc aggttgcagg agttcgccgg cccgtaaagg aagcggcgct
     3121 tccggagatc ggacagcgtg aagcgcgacc cgttcgcggt gagcgtggag agggtgaccc
     3181 cgaacgggct gaacaagtcg agcctgacgt ggtcgggcgc ctcgaccaag tacagcacgc
     3241 tgccgcgcac gcgcccttcc gcgccgaagt agtcgatctt ggcctctccg ctcaagccgc
     3301 ggctgcacga cacgctctcc tgcatgcgat cgagcgccgc ccgcgcgctc ggaaagcgcg
     3361 aaggcggcgc gtctcggtag caggatgcga gcagcgacgt cagagcaagc gcgctcgcga
     3421 tctgcttcat ccgaccttga gcccgtccag gagctccgta tccggtacgg caagcttgag
     3481 ctgcagagcg ctggtagcca cacaatgctc ctcacaggcc cgcgcgagca gcaccccgtc
     3541 gagcacggtg accggcgcgg ccgcgtgcgc ggcggcctct tcacgcgcac ccgaaagcgc
     3601 ctggcccgtc gtaatcagca ggcccgcgac ggcggggccg taatggtgga gggccccccg
     3661 caactccgtg acgcgctcgc ggccgatctc acgaccgtcg cggcgcacga cgattgcggt
     3721 gcgcgcctcg ccgaacaggc tgcgagcttt gccggagagg tgcagctcgg ctccgtgcga
     3781 accaggtcgc cgtaccgcca cgagctccgt gtatcccatc tgctccagca gcaggaccac
     3841 gagctcaccg agcgcgcgct gcggcaactc ttgcagggcc ctcaacagac tgcgccggga
     3901 cgcctctcgg taacgctcga gcgcgttttg caggtcgcgt tcagcgcgag acagctccgg
     3961 atccacgagc cagtcggcga gcgcgacgcg attgccggcc aagcgaaagc gcggacgccg
     4021 accttcaccg gcgcggcgcg cgttgtccgc gcgcacggcc gtttgcacga gcgcctgcgc
     4081 cacggctggc tctcccgtca agcgaccgcg gcgctgggcg ctgtcaatca gggattgcat
     4141 cgagaccggg ccccgaccgc gctcgaaggt acccaacagc gtctcgatgc cgtcagccaa
     4201 gggcttgcca gacagatcat tgagcggggc tcgaacgtct ccctcacgtt cggaccgcgg
     4261 ctcgcgtagc tcacggtccc ggacctcacg atcacggtcc ctctcttcac gcggctcgcg
     4321 gccttcgcgc gcagcacgag tctctcgagg ctctctcgct tcgcgtggtt cgcggccttc
     4381 gcgcggctct ctcgcttcgc gcggctcacg gccctctcgc ggctctctcg cttcgcgcgg
     4441 ctcacggccc tctcgcggct ccctcgcttc gcgcggctca cggccctctc gcggctccct
     4501 cgcttcgcgc ggctcgcgtg cctcccgccc ttcgcgcggt tcgcgcgcct ctcgcggctc
     4561 tcgcgcctcc cggggctctc gcgcctcgcg ggcgggctcg cgctcgactt cgagtagcgc
     4621 ctcggtcagc tcggccggcg cgtcactcac cgtgtaagac ggcaactcat cggcatcgcc
     4681 ccgatcgtca cctccgcgcc cccgacgacg acgacgacga cgacgacgac cgttcacatc
     4741 cgcaacgcgc tgtgcgtcgt ccgccgcagg ctcggcctct tcgccgaaga ttggacgatc
     4801 gtcgtcctcc tcggactcga aaagatcggt ggcgtgcgcc gccaactccg cgcgctcgcg
     4861 ctcctcgtcg tcggggctga gcgtttcgcc ctcctcgttc atcgtatcca ggctgacgtg
     4921 ggaagcgccc gaagcctctt gctcggtctc gaacgcgatg tcctggcccg acaacttttc
     4981 gagcgcgggc gtgcgatcgc ggaggccctt gtcgatggtt tcttgatccc actcgcgcag
     5041 cgcgaagacc cccggcttga ctcgaaccag cgggttctcc ttgtcgcctt tcttcacgag
     5101 cgccgcgagg cgcgctccca tcgtgacctc ggggctctta ccaacgtggc tcagcagatt
     5161 cttttctatc gcgacgtcag taatttcctt gtaatgcagg ggcttgccca cgaggcgcag
     5221 gacttgcgcc gcagcttccg taaacgtcat cagagagtgt ctttcggttt gaggagaaca
     5281 tcgagttctc tcggtttcga gacgcctccg gggaagcgcg gatcgctccc gccgccggca
     5341 ggccgcctcg cagtgagccc tcaatagcac ggccgaccgg cagtcgggcg gcaaattccg
     5401 cgcaaataga atacgggccc atcgaggcca gttgcccgaa cgaaggccga cgaccacccg
     5461 cgagatgcga cgaaccgcct ataaacaagc cgctccatca ggctctcggc ggatcatccc
     5521 gccggccccg ggctcgggtg ggctcggtgg ctgcgagaac gcagggcatc gaagagccaa
     5581 cctacattgg agcgccgcca ggctacactg agcccgtggt gcccgcagaa ttgcgcgatg
     5641 agctgctgct ggtagccagg agtgccgggc tcgagatccg taacgtcgaa atgcgccgaa
     5701 agaacgccgg tcctggaggg ctgtgcacgc tgaacggcga agccgtattg attctgaatc
     5761 agcgtgcctc cgcgatcgaa caagcgacgg tattgggaga tgcgctggcg gggcgcgatt
     5821 tgacggccgt ggccatgccg tcgtacgtgc gcggcttcat cgcaatgcga cagcgcgcac
     5881 gctcccgctt gttggtcccc gagcgccgac cagggcccgg cgtggttcgc ggcaacgtgg
     5941 agacgttccg gcgccgacga ggtgatccat gaagctgctt tggagtgccg tttgttgatt
     6001 cgaaatgacg tcccgctcgc cgactacacg acgctcaagc tcggtggccc cgccaagcac
     6061 ttcgtcgagg tacgtgatga gaacgagctc gccgaagccc tgagctgggc ccgctctcgc
     6121 aacgaacgcg tgttcgtgct cgggggaggc agcaatctga tcgtgcccga cgcgggagtg
     6181 gatggcttgg tggtctccat cgcgctgcgg gggattgcgc acgacacggg caaagcgggc
     6241 agcacgctgc tgcgagtcca ggcgggggag ccctgggact cggtggtcgg ggttgcgctg
     6301 gaggcgaacc tcgccggaat cgagtccctg agtggaatcc cgggtcaggc cggcgcaact
     6361 ccgattcaga acgtgggagc gtacggtcaa gaggtcaaag acacgattgt acgcgtgcgt
     6421 gtgttcgatc gagaggatcg atgtttctcg gaattgaccg cggccgagtg cgagttttcg
     6481 taccggcata gtcgtttcaa aacgcgagat cgcgatcgct tcatcgtgac acgagtggac
     6541 ttcgaattgc agactcgtga ccgagccgac gtgcgctatc cagagctggc tcgggcactg
     6601 gccgaacgag gacgagaggc gtccctcgtc tgcgtgcgac aaaccgtgct cgagctccgg
     6661 cgcagcaaat cgatgctgat tgatgcgcaa gatccgaacg ggaggagctg cggttccttc
     6721 ttcttgaatc cgatcgtcga accgcaggcg gcggatcggg cccaaagcgc actgggcgtt
     6781 ggcgagatgc cccgttaccc gcaaagtgat gggcgcacga agctctccgc cgctttcctg
     6841 atcgaacact ccgggttcgc caaaggcacg cgcgtgggcc acgtgggcat ctccactcgg
     6901 cactcgctgg ccttggtcgc acacgcgggg gcaactagcg cggagctgat gcagttcgcg
     6961 agccaagtgc aggacggcgt gcgcgcgcgc accggcgtat tgctctcact cgagcccgtg
     7021 ctttggtgag gggtcgcccc gtcgcgaggt gcgaccgcgc gccgacgatc gagggccgtg
     7081 cctgaacggc ccccgcgtag cgcaaacgga atgcgcggac ccgctcaatt cgcggcgggg
     7141 acgtgctggt acaaggcttc gatcgcttcg tccagacgag ccacgtcggt gccgccggct
     7201 tgagccatat ccggacgacc gccgccagac ccacccacga tttgagcgat cgggcggatc
     7261 agctctcccg ccttgacccg gctcgagagc gcgcgtgaga ccgtcagcac gagctgggcc
     7321 ttgccctcgc tcacggagcc gaccagcacc acgctctcac cgagcttgtc gcgcagcttc
     7381 tcggccaatt cgcgcagcgc cgcgcggtcc gtcacctcgc tgcgcaatcc gagcaccttt
     7441 acgccagaaa catcgcgcgc ttgcgcgagc caggcgtcga agccccccgc gccccccgtc
     7501 gagagcttgc gcgtcaggtc ctcgatttgt ttctccaagt tgcgctcgcg ttgcagaagc
     7561 ttctcgaccc gctcactcag ggtgttcggc gaagtcttga gtaacgctgc cgtgcgcgac
     7621 aaagagctct ccacctcgcg tacgtaggcg agcgcgcctg ctccggtaac gccgatgata
     7681 cggcgcacac ccgccgccac accctgctca ctcacgatct tgaacaagcc gatatcaccg
     7741 gtcgctcggg cgtgggtgcc gccgcataac tcgagcgatt cccccatact gagcaagcgc
     7801 acggtatctc cgtacttttc ctcgaagatc atcatcgcgc cccgcgtgcg ggcctcatcc
     7861 atggtcagca cttcggtgcg gatcgggtga ttctgcagcg tgcgttcgtt gacgagctct
     7921 tcgatacgcg agatctcctc gggtgtgagg ggcttgccgt gcgcgaagtc gaagcgcaaa
     7981 cgatcgggcc ccaccaagga ccccttttgc tgcgcgtggc tacccacgac cttgcggagc
     8041 gcccagtgca acaagtgcgt cgccgaatgg ttgcggcgag tccgctcgcg ccgctcgatg
     8101 tcgaccttga gctcggcccg ttctccgacc gcgacggtgc cttgcgtgac tttgccgcgg
     8161 tgcacgaaga gccccgaaat cgggcgcgaa gtctcttcga tctggatctg cgcggcgacc
     8221 gtggtgatca cgccttgatc cccgacttga ccgccggcct cgccgtaaaa tggcgtgagc
     8281 tccgtgacga tctcgacctc gtcaccggtg tgcgccacat ccaccatctt atggtcgcgc
     8341 atcatggcca cgatgcgggc ctcgtcgcga tctttgtcgt agccggtgaa gcgcactgcc
     8401 gcgccgggta ccagcgccag cgcttcgcga taaacgccct cgacggcttg ctccacgcct
     8461 ttgaagtcgg attgctcgcg cgccttttcg agagccgctt tgtaaccgtc ctggtcgacc
     8521 gtaaatccct gctcggtgca gatcacttca gtgaggtcga gcggaaagcc gaaggtgtcg
     8581 tagagcttga aggcctctgc acccggcagc tccttggaac ccttcttgcg caactcgtcg
     8641 aagctgatgt cgagcaagga caagccacgc tcgatggtcg cgcgaaaccg cacctcttcg
     8701 ccttcggcga gcgatgcgat caagtcccga cgctcgcgca gctccgggta gtgatcaccc
     8761 atcacgtcca ccacccgcaa cgcgacctca tggaggaagg gcttctcgat cccgaggcgg
     8821 tgcccgtggc gaatggcacg gcgcatgacc cggcgcagca cgtaatctcg ccccgtgcgg
     8881 tccggagtga ccccctcggc gatcagaaat gcggtggtgc gcgagtgatc ggcgatcacg
     8941 cgcatgctca cgtcatcggg gctgctcgtg cccccgtacg gcttaccgga taggcgcgcg
     9001 gccgtctcca cgagctcacg caggatatcg gtatcgtagg ccgtaccctt gccctggagc
     9061 atgcacgcga tgcgctcgag cccagctccg gtgtcgatgc tcggcgccgg tagtgatgcg
     9121 agcttgggag atccgtccgc gtcgaggctg cgctcgaact gcatgaagac cagattccaa
     9181 aactccatgt agccttcgcc ctgcgcggtc tgctcctcgc cgaatcgcgc cgggtcgggc
     9241 ttgcccgagc cattgtagaa gtagatctcg ctgcacggcc cgcacggccc ggtgtccccc
     9301 atcgaccaga aattgtcgct catgccgagc ggacgcacgc gatcctcgga gaaccccgtg
     9361 agcttgcgcc agagatcacg ggccaccgta tcttccggga tgccctgcgc gcccccaaaa
     9421 tacgtcatta ccaagcgatc ctggggcagg cacagcacct tcgtgagaaa ctcccaagca
     9481 aaagcgatcg cttcttcctt gaagtagtcg ccaaagctga aattgccgag catctcgaag
     9541 aacgtattgt gccggggaga aggccccacc gcctcgaggt cgttgtgctt gccgctgata
     9601 cggatacact tctggctcgt ggtggcgcgc cgataaggcc gccgctcacg tccggtgaac
     9661 acgtccttga attgcaccat gcccgcattg gcgaacatca aagtcggatc gttctgcggg
     9721 attagagaac cgctcttcac gacctcatgg ccctggccgc gaaagaactc gaggaacgct
     9781 tggcgaattt cagacgaggt cgtaggggta gtcatgagct gggtgagcat tagcactact
     9841 gccccgcagc tggggaacgg gaacgatggg aaaagtggag ggtttgccac cccctgccgc
     9901 cactgcgcct cctgagctcg cggcgttcat ggccgagcgt gggatcggcc ccctcgaacg
     9961 cttcgtccgg ctcagcggca ggctcaaact gcccgaagtc agcgaccaag agctcagcgg
    10021 ctggaccctg tgcctcagcc aacgagggtt gtggctggtc cagagttcag gacgattctc
    10081 ggggcacgtg ctcgagctga cgaatcctga tttcgagtct gcgcgtcgcc tccgttatga
    10141 gtcgggccgg ctccgggatc gcctagtgat agacgggcag ccgctccgtg tgcctcccaa
    10201 cgcgggacgg cgcgcgcgcc agctcattgg ggtggggcga attcgtgcac agcgcgatgc
    10261 gaccgagcct ggagggcggg gagccctgat cgaaggcacg atcgagcccc cacagcagct
    10321 ggagctcggc caggtgggcc cccttgcccg gcttttcctg aatgggttgg ctcgccccga
    10381 cgagccgtgg ctcgtcatca tcgagctcgg tgagagtcac ctgctttcgg cgctgcagac
    10441 caaggcgcgc gcgggctgct acttggcgct gagccccgag cgcatcgtgt acgcgcgcgt
    10501 ctccgacttg ggagattgcg agtgcacgcc actggatccg agcgcgctcg agctcgatac
    10561 atcgaacggt gatgctcagc tgcgtgcggg ccaacgactc attccggtac gttcggcggc
    10621 aacgcgcgat attgcggagt ggctcggtct cggcgggctc gagcgagccg agcgcctgta
    10681 cgaggcagct cgtcgctggc acctgaaact ccccccgaac cagcgctcgc aactcggggc
    10741 cgccagccgc tcgggatggc tggatctcgc gttccgagcg ggcagcgtga gagcgggctt
    10801 ggttttggtt tggctcgatc gcgctggccg cttcgcacca ggtggagaag cggccgcacg
    10861 cttggccttg gtcgcaagct cgctcgcggg gtccgagggc agccgcgatg aagaagggaa
    10921 aagctactcc gatcttgggg cgcgctggga ggtgccgagt gacgaggccc ggcgctggct
    10981 ccacgatctc gagaggctct ccgcgccgtt gccgcggctc gcgctcgagt tgcatcgcgc
    11041 cgaagccaag cgctggctcg aggtaactgc ggcggcgccc ccgcccgttc acgtcgaaat
    11101 cgagctggcg gagcacgagc tacgagccgg atttccggag catgcgcgcg cgctcgcggg
    11161 cgtacggctg gcccgtttgg aggacgacga gagcgccttg ttcgaggccg gcctggaaca
    11221 actcgagcga gcgcgttggt tcgagctcct cgcgcgagag gcggagcagc gcaacgagag
    11281 cgacgtgcgt gcgctttccg cgctggcgcg gttgcagcct caagagagcg cgcgcctcga
    11341 acggttggcc cgcgcccatt ccccggaccc gcgtgagggg cgaatcatcg aacgtgcccg
    11401 ccaggtgctg gtgtgtctcg agccaggagg tctcgcgtcg cccgcagtaa tcgccggcga
    11461 tcggctcgtg cttccgctca gcaccacaga gcgggagcgg ctgcgccatc cgctggcgcg
    11521 cggcagcgct cgagtgagcg cgcgcctttc cgagctcatc gccaagaccg cagagcccga
    11581 cgtcggcttt cttcgcgact actgcgaagt gctgagcgac gaacgccacc ccgaggcaag
    11641 tcgagccttg cggcgcgcgg tcaccttctt gagcttgccc cagctgaagg catacatctc
    11701 gcacggcgcg cgcagcttgg gcttacgcgg cttcagcgct cgaccgccgt tcgtgttgat
    11761 cggcgccagc catttggatc cgaacaatcc tgcgcgcctg cacgatcgag aattcgactt
    11821 cgcgtttgcc gcagaactgg cacatctcgc gttcgatcaa aagcgcgtga ctacgagcga
    11881 agtactcagc ggtgcggccg gcaaaacgcg cgaagcgctg tccttgctcg gcctggcgct
    11941 gccgctgatc aaagaggtgt ccgggggccg cgctcaaaaa gttctcgacc gagttagcaa
    12001 ggaagcactg gaccgagccg cggagggcgc cgcgcggctc cccgaactgt tcggctctcg
    12061 aacgagcgga gagggcccca ccagcacgct ggcacgcgct aacgaagagc tgatcgcggc
    12121 gcatcgcttg gttcagctca ccgccgatcg aagtgggtta gtctgtgcaa atagtttaca
    12181 tgctgccctg cgttgcatgc tgctgacccg ctccgactat cacacgatcc tggccaccac
    12241 cgcgcaagca ggcttgcgca aagctctgtt cgagtccggg agccaagacc ccgccgcgca
    12301 ggatctcgcg ttccgggtgc gagcgttgat tgcgttctac ctgtctgacg acttcgacac
    12361 gctcactgca tccgattcgg gctctgcgga atcggtcgta gatcctggtt gagcgtccgc
    12421 ctcgactgcg ctcgtggatc cgtcgatacc gcgattcgcc aggcgatcgg cctcactgtt
    12481 gagctctcgt cggacgtgat tcaactcgaa gcgctcgaac ttccccagca gctgcttggc
    12541 ggcttcatag aggggcttga ggccgtagtg gcgtacccgg tactcccccc gcagctgctt
    12601 gattaggagc tccgagtcgg cacggacctc caagcggcga actccgagtt ccagtgcgcg
    12661 ttcgagtccc agcagcaaag ccttgtattc cgcgacgttg ttggtggtcg tgccaagata
    12721 ttcggtcacc tcggccaaga tgttgccgtc tcgggtcttg atcacggcac cggcgccggc
    12781 agggccgggg ttgccgcggg ccgcgccatc cgtccaaacg atcgcttcag tgacgtggag
    12841 tggtgtctcc tcggacatat ctcgctcttg ggggttcgac aggtacgttc ttcctgcccg
    12901 cccatgatcg tgtgctctgg agtacttcgc gcaatacagg cgcgacggcc ccctcgtcaa
    12961 tcacactcgc cctcggtctc aatctgacgc gcgagggaca gctcggctga gctcgcgata
    13021 cacgctctcg tactgcgcgg cgacatgttc agcagatgca ctatttcgca cgaactcgcg
    13081 ttgttctcgg acgcagcgcg ccgcgcgatc gcactccgat accaagcttt gcaacgccgc
    13141 aatccagcgc tcgggatcta acccctggac ctgagtcgct ccacggagtt cgaccaacgg
    13201 tcccgctgcc agcgtgacga cgggcacccc cagcaccatc gcttcgagca acacgatcgg
    13261 caggtcgacc tttccgtata ggtcatcgac cggaaagatc acgccatggg aggcggtgat
    13321 caacgacagc acgtcggggg tcgaatccac gaagcgagca cagcccgagg gcaaacgcga
    13381 tcgaagcgcg ttggcgatct cgagcgccct cgccgtcttg cgacgaaatg caaagacgat
    13441 caccgccccc ggtagcgcag cgccaattcg ttccgcgatc tgcgcgctca cctcggcacc
    13501 cgagctcgtc tccaaatctc ccggataaac gaaccaaggc gcgtcgccgt tcaacccgag
    13561 ctccgcgcgg gccttttcgc gctgctcttc actgcgggtg atggccatgg aaaccggcgg
    13621 caggatgacc ctcacgtcgg gcagaggcgc ccgggccgct gccgccgagt cgcgcaccct
    13681 ttgccgcatc cactcgctct gggccaccac gacgtctccg aacaagagcg actgcacgtc
    13741 ttcgaagtgc cgcgggcccg acgcgatggt ctgcacgacc ggcactcgac gcgcgcggga
    13801 cagcacgcga ccaaccgtgc tggtgcgcgg attcggcgcg aacacgaagt gccaaacgtc
    13861 cgctcgagat gccaacaaga cccacagcgc ggcgcggaaa ttttgcgcca agagcggcgt
    13921 gtaggcccct gcgtccgaat agaccggcac gctcgcgaca cgcgagccgt cgatccgcga
    13981 ccacatcggg ggcgcggtgc gggtagacaa aacgcacggc tccaccgctc gcaagtgagt
    14041 agcgacgtcc cgcaccagac acttcgctcc gtcgtggaag ggcggcgcca tcggtttgga
    14101 aatgaagagt acgcgcggca aaggcgggga ccctaatgcc tcgccttgca gttgacaacc
    14161 cgcgagctga acgagagcgg gctcgagagg gataggcttc ctcctggatt tcgtgccaca
    14221 acttgccgat gccgctctgg cccggccgcc ttcgctcgcg cttgttccta ctatggatgg
    14281 tcaccttcat gcttacgagt tgtaagcggg aagcgagcgc cgccgatccc cccgaggtca
    14341 agcacggccg ggagctgtac gggcgcatgt gcagcgtgtg ccacggtgac aagggcgagg
    14401 gatacaaggc ggatcaagcc ccccggcttg cccaaccgga gttcttgggt tcggcgagcg
    14461 acgccttctt gcgggcggca atccgggatg gccgccgcgg gaccaccatg tccgcgtggg
    14521 cgcagagcca cggcggaccg ctcacggaca aggacatcga ggacgtggtc cgttttctac
    14581 ggacttggca gaaaggcacc cgggtgaagc tggatgaatc gccgctgacc ggcgacgtgt
    14641 cgcgtggcac ccagctcttc acgcaacgtt gcgagctgtg tcatggcaag caaggcgtcg
    14701 cgggtctgaa tgttcgcatc ggagctgctg attttctcgc aaccgcgagc gacggctttc
    14761 tccgccacgc catcaagcga ggacggcccg gaactccgat gaaagggttc gagagcagct
    14821 tggggaagca aggcgtggac gacgtgatcg ccgcgctgcg cagcttcgcg actccaccgc
    14881 cagtcgccgc accggagccg cccaagcctc cgccgcccat gcccctcggc cccgttccga
    14941 tgaaccccaa gggtccggag ccgcatggct tcaagctgta cccagcgatg accggcgccg
    15001 acgtgatcaa ggccgaatat gatcgcaaat cgcggttcgc gatcctcgat gcgcgagccc
    15061 cttccgacta tctgaacgaa cacatcgcgg gcgccgtcag cgttcccttc tacgaccctg
    15121 ccccgtatct cgacaagctg ccgcgcgatg cttggctcgt gtgttattgc gcctgtccgc
    15181 acgccgaatc tggccagctc gcgcagcgcc tactagacgc gggattcaag aaggtcaccg
    15241 tcttgaacga gggcctgctg tactggaaat ccaaaaacta cccgctcggg cgtggtgaag
    15301 ccaagcccgc gggcgatccg ccaaaagcgc cgagcccctg aaccggcgcg agagcgttga
    15361 cgatcgagcc cagctctgcg aggagaaggc atggcgttta cgacgaccgc gagtgggctc
    15421 tcttacgaag acacgaaagt tggcaccgga ccctccccgg gccgcggcca aacctgcgtg
    15481 atgcactata ccggctggct ctggcaggac ggcaagaagg gggcgaagtt cgatagctcc
    15541 gtcgatcgtg gccgcccctt ctcgttcccg ctcggtatgg gccgcgttat tcgcggctgg
    15601 gacgaaggcg tggccgacat gaaagtcggc ggccaacgca cgctcttgat cccgccggat
    15661 ctcggctacg gtgcgagcgg cgccggcgga gtgatcccgc cgaacgcgac gttgctgttc
    15721 gaagtcgagt tactcgagct gcgctgagag ccggagcctg tcagccggcg tcgcctggca
    15781 gtggcgccgg ctcgatctct agaaacggga tgcggcgagc aaggcgcccg gagcacgcga
    15841 cagcgcgctt tgaatcgcgg cttcgattac ttgtcggcgc agctctgcga tccgcgcgcg
    15901 atccttcgtt tcagcgaacg aagcggttgc actggctgat ccggagagcc gcgcggaaat
    15961 ggcctcgtcc ggcatactgt gaaccaagaa ctcgatcgtg gcggagtaac ggataccgtc
    16021 tggttcgcgc tctgcagcga gcttctggat actcggaacg atcaacacga cgcactctgc
    16081 gttgttttcc gcgagcttgt cctccgagac cttggcggcc tcgcctgacc gagacctact
    16141 cgcttttgcc ggtgcggccg tgctcgaggg gccgtccgcg ggcgaactgc ccacccggct
    16201 ctccacgacc acgtaaggga cctcggacag ccgtttgcgc gccgagttca gtacatcttg
    16261 ctcgaccgac tcggacttga cccgggtgga gttgtgcacc ttcgcgagct gcaccacgac
    16321 tcgcatcaag ctctgagggc ggggagcgcg cgcgctcagc accgagatcg cgctcttgat
    16381 ctcttcgcgc acattctcgg cgccatccgc ggagtgcaag cgtagcgccg gtacaccgcg
    16441 cgggtcgccc aacccgcgta gcccaaccgc tgccgcggcg cgtaccgcgg gcgcgtggtc
    16501 atcgagcgcc agaatcaagg gcttgagcgc gcgcggatcg cgtgtcttac cgagcgcgag
    16561 cgccgcctgc gctcgaaccg tcaaatctgg gtctccccga agacgatcac tcaacgtagt
    16621 gaacggaggt gccgcgcttg aaagggtcgt gtgcagcagc atgcagcaca accagccagt
    16681 tacgagtgta agccgcatcg agcctaattg ttcgtgatac gggtaaagtt agttcacatc
    16741 aagtttcgtg acgaccttgc ctcaattacg ctcacactct tcacaccaat gtgcgcagca
    16801 gcagctcacg ccaacctgac cgcgagttac accatgtgcg agcagcgagc cgaggaccat
    16861 gctgagacgc ggagagcttt tatgctcgca gcgtgttttt cgcatatttc cgaccatttt
    16921 cgcccgaagc tcgagttcaa gggcctagcg tgtatttgcc tgggagcgat gcatgcaaac
    16981 cgctacttca atgcacaggc gctccatgag cagctgtcta tgctctgcac tttaattcgg
    17041 cgttttgcgt tgactgcgcg aaaccagccc gcgtgtgcac agccgaatat gcacagcgga
    17101 atatgcacag cggcacgagt tcagtgttga agcgagcttc acactgaagc actcgattca
    17161 tcggtatgga gcgaatcatc accggggccg atcggtgaga tagtcacttg gatagtagcg
    17221 aatgcacttc gttacgaatc tgagcttcgt caccttcgga atagcccgca tggacaaagc
    17281 gcacgatccc attctggtcg atcacgaagc tcgtgggcat cttgggtgga gtgtaactct
    17341 gtgcggcaga ctgcccttcg tcccaaccga ttggaaactt cgctccggtg gcgcgcacga
    17401 acgccgcgat gccgctcgag ctctcgtcta cgctcaccgc aaccacagct agctcgccgt
    17461 gaaactgctc gtacagcgat tggtatgcgg ggaaggactt gcggcagggc tcgcaccagg
    17521 tagcccagaa gtcgagtacg accaccttgc ccgcgtaatc cgcgaggttg acgcgaccgc
    17581 ggccgctcgg aactccaacc tccaagggtg gagccgcaga tccgagcaaa gggtgatcgc
    17641 tgacggcgtg tgccgacccg acaccagagg ccccgctcgc gcacgacacc acccccgtga
    17701 gagttgcgca tagatacacc gcgaggcaca tcgaaaaggg gttagtgagc gacgcgcgag
    17761 ccgacacctt catagacgtt ccagcggaga agcgtagctc gaacttaaga accttacgac
    17821 gccttgcgcc cggtcacgcg aggaccggag acatcggcga tgctaccgct ccggtacgga
    17881 gctagctggt gttcgcgcca cgttggtgct tccggtgtgc cgagcgggcg cggaccccac
    17941 gcttgggggt aaagattgcg aacacggcgg tcgcgagatg gcgaccgccg tgaagggccg
    18001 actcgagctc acttctttgg ggcgggcttg gccgctggcc gacggccccc gcgaggatct
    18061 tcatcggggc agccgtcttc gtcttgatac ccgttgaggg tctccggctc gtcgatgcac
    18121 tcgtcctggt cgtctaggat ggtgtcgctg tcgttatccg gatcggggca gccatccgtg
    18181 tcgtcgaagc cgtcggtgtc ctcggcctcg ttcgggcact tgtcccgggc gtcgggcacg
    18241 ccatcgttgt cgacgtcggc ttcgtcgggg cagccgtctt cgtccttgta gttgttcttg
    18301 gtttcagcct gcagcgggca ctgatcggcc gtgtcggcca ggccgtcctt gtcgttgtcg
    18361 agctcgggac aaccgtcagc atcctcgaag ccgtccgcgt cctcggcttg catcgggcac
    18421 ttgtcggcgg catccggaat gctgtcgagg tcgttgtcac cgtcggggca gccgtcgccg
    18481 tcctcgtagc catctcggtc ctcggcggca gccggacatt gatcggcttt gtcgtcgatc
    18541 ccgtcgttgt cctcgtcgct cttctcgctc acgaacgtga taccaaacat gccccgaacg
    18601 ttgggcgcgc ccacgccgcg agctacgccc gt
//
LOCUS       DASTKU010000264        10811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1836777, whole genome shotgun sequence.
ACCESSION   DASTKU010000264 DASTKU010000000
VERSION     DASTKU010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10811
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836777"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFQ61_09545"
     CDS             complement(<1..400)
                     /locus_tag="VFQ61_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954739.1"
                     /translation="MSRSARDQESRRTVVFLFDPAAEPASLPCQLRSLGPCRSNLRPS
                     AEQAAAGKPPYRCAVYQRVAERCPCQAGPDLSHDLILLFPFNLARANSLPHRYTLESE
                     DGTLSRERALARDARANEQQRAELTFEHLPE"
     gene            complement(381..1742)
                     /locus_tag="VFQ61_09550"
     CDS             complement(381..1742)
                     /locus_tag="VFQ61_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9954740.1"
                     /translation="MLPREGVMVPLGGARVRLREREIGAEFGHEQRARNVRIAAELCR
                     MLERGVEDLDWLAVALTLQGGRAPASRQMSRRQMQQWIAGVAREAERALAGGDWVVDP
                     FTAPSDGVPEGASGSVESERAGEPAPRRRRPEAAEKELDWFSLRVIDEVGAPVDGARV
                     ALEISGTERVLTTNSVGLVRVDDVPDARQAKARLLDRQELTTLLKPRWQVPRAPRPFQ
                     GSHVHTKNLEQAFLDELALPAETPVTLVLTPYFECREVPGAYFEFGRSFVRSDALEVL
                     AGTAEALRSDAGLKAIVFGHTDVTGSEALNKELSERRAKAVHALLTHDAEAWEQLFSG
                     HGDGANWTEKWDLKEAQHMLNALGVTDDAGQPVPEHGRRDAETVQAIHRFQRGEYPDR
                     PAEQQPLVEKDLLGPEGRRELFLAYAKRVGRTPVEKERFVRIRWGSFHGLRRVQPRES
                     ERS"
     gene            complement(1896..3086)
                     /locus_tag="VFQ61_09555"
     CDS             complement(1896..3086)
                     /locus_tag="VFQ61_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954741.1"
                     /translation="MKKNVMSGPAASAHEIQWVDSTRASRSRVKYRELRRLGRMGDGQ
                     ESGSTFSAALLLFGDQILSVGERTLAFNRRSGAVVWVKEGGWPRAALVGERVAYPCGT
                     PVLQSGQEENEQLLFRNSVREWGRVRRGHAILVSQYPTNRHEGINATVLVVRRAIVSD
                     VDTPDPEGYGDAGYVFKDAYVSAAIFDSGDVVLVARDGRFVILDAAGQKKMVVRKQGQ
                     LGFRSYDLSVGAEHMALLEAPESWTDSDLDDAEPRPLAELSPDPVPPIPPPPPQWTTG
                     LRVLNLEGRELQRASVPIAVVEPPIDGGEGRFWLAGYGLALVERGQVVFAQRSEHLFL
                     GTAFASGELALSAYNQVQILSKSGAVLQAFRVPKGEHLTSAPTIDDDGSVWVTTNRGI
                     YVAR"
     gene            complement(3278..6406)
                     /locus_tag="VFQ61_09560"
     CDS             complement(3278..6406)
                     /locus_tag="VFQ61_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954742.1"
                     /translation="MSGNDVPALMPTGPVTRTTAGLVVPLAAARFVIRGQFFDPRRGQ
                     DVPLPDHQFGLLNLKPGSLAPIFGGPSNKDGISWIEFEDDLRFDGVDPFAEWELAWRP
                     LFRNTAGRLRPALDVQRSGEVFLDLDKQEWVLPREVGRLDARRLIRLPVWSSSIKVLV
                     GGFLEGVPKTRFAETGVFSMARGESVAHGTLVKPWVVQVDHGLFRNFVRFHYYDVANK
                     KRAAVPSHVVVEALGRPRLSPGHEPPSTDQKSVLQRRVAAGTPIDDSGNVYLLHERTE
                     QDSGDVHYYFATVERGQDGQGVLDADAAPAHRVQVKAGAAVPDNALLANGGVRRPASH
                     YYLPREWHSHGMLAFIGAPDGQPVERREFEALRTRGVERASPLCFHLDDMVLADENEF
                     ALQLNGKSRVALFDHWLHIRDPDPARPHVWRTLPQKNYFPAETAVVQEGQGIERRTRL
                     VYHDGRFHDLHERRMTIKSESLLGHTDCVGARVALLDDSTGRRLADYMHGIPNQTVVF
                     GSAEYHLIDASFMDHEFPATTSGAVNMKLMHLLVHIPLRLDGSRVSGAELDRMLPALT
                     HAAERWDQKHPGHPNAPPPDRVPCTMGLVPLTGAAAGDKVVRVRHYFGEGPSRADAVV
                     VHVRPAGGRASAGHYTPEITVFDGDIDESRTQFQAHLDDERRQNPAFARFTLAHELGH
                     KLGWPDEYDELMTGLASFSALRGRFLGLQQAIEGVIARPFATDEPSIMNSNSHPRLRH
                     YWIYANRLREEAKFREWLDGKDYVAIDTQTVPGTLMSSELPFVGPSVLPDEANHAWYR
                     PVAGPRPLHATNRADIAVYRLPQDQGTVRKMFLGPGGTRDTALPPNQRYDGIALVYTR
                     ILFNFADSLGETKRLECLEAIVFSARLMDVRFAYRKPKTGHLDYVERIRYMLQVRSAR
                     SNRPPTAEPCLQRIALSVSPLIESVQELAQNGERRPRDPHLTIDVVEQRESPHPLLDS
                     RHTGNLVLTPSEVVAFPVAILCYALAAKPVDANGRLKVRFSASDFARVATSVSQLLHD
                     PPGVQRAVESAP"
     gene            6813..7238
                     /locus_tag="VFQ61_09565"
     CDS             6813..7238
                     /locus_tag="VFQ61_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954743.1"
                     /translation="MGRGPATGEASNTNWDRRMQVDIVPSGVVSLEMMRDLGELQEEL
                     TEAGLDARGSHASIPGVKDGGLTIAIGIAGVVLSAVSSLVSVLTYWSSRKPSYSVKVE
                     AGSGTLTISDLDPSSVQDVLKRLKEVDPKSGLLVRVTRR"
     gene            7246..9855
                     /locus_tag="VFQ61_09570"
     CDS             7246..9855
                     /locus_tag="VFQ61_09570"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9954744.1"
                     /translation="MGRRLALVVSADSGSELSGGKRDAMRVFSVLADENLGACDPESP
                     QPIAACSSRARFHEAWHSLLTNWNPQDQLLFYFSGHGVMKHGRYTLAFGAAPSQHYVP
                     FDHLTSDLQAHGVRRAVVILDACHSGAALQAGAKGTDGELPIQTLPAGIVVLTSCRSA
                     EQSFEFEDGAGSVFTHLLTQGIRSGLGGKPTPDGLIGPDDLVEYINTRLAEPEYANYP
                     QRPSYQVSSADRTVWIARNRSVAPAEARPKVATRAYSIEQLHLLYDRTEKTRHPCPGA
                     TLDCLDWDLVAQYAEASRTPLESTESTESRDQAARRLGLYSSLSDDVLHNAAVLCFAR
                     APHALLQQGRALYLRGERSSTHALRTDVLGPLSQQVRELTQLVMTDVYRRWGWRPTDP
                     TATLLAETVREAVSNAVAHRDYDRSELVRVSMRDDCIEITNPGAFPRGTTFESLLAAG
                     NYSEPNDAAIAWYLTTLLAYEGVGRGFSVYNAFRAAAGDNALECHVQAELRSIRLVLR
                     IPTQIAPQLAPVPEKTEPPAAVPTGPGTLHGLGRDVVFGEPIHSTVAQPNLIPRTGTE
                     ELVGQRYRLGPTIGTGASGTVFLAEDTRLARQVAVKVLSKRFVTAEVRERFLQEAKLA
                     ARVVHPNVVAVYDVGEETDRLYIVMELVTGDNLDATIRGSERLREREPDLFFLQAAWL
                     IYDLAAALAAAHASLIIHRDIKPSNVIVEQSGSPRLLDFGVSRAASRDTDSRLTATGV
                     LVGTPYYMSPEQVRGEIEDERSDIYSLGVVMYELLCGRLPFEAQALPSLFIDITQGDL
                     VQPRRHAPRIPVELESICLRAMQRERVRRYPQADSMALELRQYLPSNPCHRLYSQDRA
                     KLWADFKARLDKA"
     gene            complement(9900..>10811)
                     /locus_tag="VFQ61_09575"
     CDS             complement(9900..>10811)
                     /locus_tag="VFQ61_09575"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HET9954745.1"
                     /translation="APRMASALALVCFMFVAGYCARRFNRLPDNAADVLNRFVIDICV
                     PATILRLVPKLTLGSHLLLLAITPWFFAGLAWAVARVAQKLLRLQREHTIALFLATSL
                     GNTSFLGFPLCQALLGEAAVPLAAVYDQLGSFLLLGTVAPVALARATAGSAPKWSELL
                     RRVLQFPPFLALLLALLLAALHVPHMAWLDVLLIGAAAPLVPLAMFAVGLKLRFSAPR
                     PRRVFALGLMLKLAIFPLLAWGISALLHAPPLVLKVNVLETAMPTMITAGALMMAQGV
                     AGELAAAFVGWGLVLALITIPAWAAIL"
BASE COUNT         2111 a         3563 c         3231 g         1906 t
ORIGIN      
        1 cctcgggcag atgttcgaag gtgagctcgg cccgctgctg ctcgttggct cgcgcatccc
       61 gtgcgagcgc gcgctcgcga gaaagagttc cgtcttcgct ctcgagcgtg tagcggtgag
      121 gcaacgagtt cgctcgcgcc aaattgaacg ggaaaagcag aatgaggtcg tgactgaggt
      181 caggcccggc ctggcacggg caacgctccg ccaccctctg atacacggca catcgatacg
      241 gaggtttgcc agcggcagcc tgctctgcgc tcggtcgcaa gttgcttctg caaggcccga
      301 gcgagcgcag ctggcagggt aagctcgcgg gctccgcggc agggtcgaat aggaaaacta
      361 ccgtgcgccg gctctcctgg tcacgagcgc tccgactcac ggggttgaac tctccgcaac
      421 ccatgaaagg agccccaccg aatgcgcacg aacctttcct tctccaccgg agtgcgtcca
      481 acgcgctttg cgtacgccaa gaacaactcc cggcgacctt cgggacccag taagtctttt
      541 tcgaccaacg gctgctgctc cgccggccga tccgggtact cgcccctttg aaagcgatga
      601 attgcctgta cggtctcggc atcccgtcgg ccgtgttccg gcacgggctg ccccgcgtcg
      661 tccgtcacgc cgagcgcatt cagcatgtgt tgagcctctt tcaaatccca cttttcggtc
      721 caattggcgc cgtcaccatg gccggagaac aactgttccc aagcctccgc gtcatgagta
      781 agcagcgcat gaaccgcctt ggcccgacgc tcactcaatt ctttgttgag cgcctccgag
      841 cccgtcacat ccgtgtgtcc aaagacgatt gccttgagcc ccgcgtcgct gcgcagagcc
      901 tctgccgtgc ccgccagcac ctccagcgcg tccgagcgca cgaaactgcg accgaactcg
      961 aaatatgcgc cgggcacctc gcgacactcg aaatagggag tcagcaccag agtcacgggg
     1021 gtctcagccg gcagcgccaa ctcatccaag aaggcttgct ccagattctt cgtgtgaacg
     1081 tggctgcctt gaaacggacg cggagctcgc ggcacctgcc atcgaggttt caacagtgtc
     1141 gtcagttcct ggcgatccag taatcgcgcc ttggcctgac gcgcgtcggg cacgtcatcg
     1201 acacgcacca gcccgaccga gttcgtcgtc agcacccgct ccgtacccga aatctcgagc
     1261 gccacccgcg ctccgtctac gggcgcgccc acctcgtcga tcaccctcaa agagaaccag
     1321 tcgagctcct tctctgcagc ctccggtcgg cgccttcggg gtgctggttc cccggcgcgc
     1381 tcggactcga cactgccgct ggccccttcc ggaacgccgt cactcggggc cgtgaacgga
     1441 tccacgaccc aatcgccgcc cgccagtgcg cgctccgcct ctcgcgccac acccgcgatc
     1501 cactgttgca tctggcgccg ggacatttgc ctgctcgcag gcgcacgccc gccttgcaga
     1561 gtcaacgcga ccgcaagcca atccagatct tcgaccccac gctccaacat gcggcacagc
     1621 tcagccgcga tccgcacatt gcgcgcccgc tgctcgtgac cgaactcggc cccgatctcg
     1681 cgctcgcgca gccgaacgcg agcgccgccc agcggcacca taaccccttc tcgaggaagc
     1741 atcttattgc cccttgctcc tatgccgaac tcgtgagggt ccggcgcagc aacgagcgcg
     1801 actccacgcc gcttcgaccc accagcgcag aaccttgtac cccccgcacg tcgcgcccac
     1861 cgcgcacgcc ccaccctgta accccggcac ctcggtcagc gagccacata gatccctcgg
     1921 ttcgtcgtca cccaaacgct cccgtcgtca tcgatcgtgg gtgcgctcgt caggtgctcc
     1981 cctttgggca cccgaaacgc ctgcaacacc gccccggact tcgacagaat ctgcacctga
     2041 ttgtatgcgc tcaacgccag ctcccccgac gcaaacgccg tccccaaaaa caggtgctcc
     2101 gagcgctgcg cgaacaccac ctgcccccgc tcgaccaacg ccagcccgta tcccgccagc
     2161 caaaaccgcc cttcgccgcc atcaatcggc ggctccacca ccgcgattgg aacggacgcg
     2221 cgctgcagtt cgcggccctc caagttcagg acgcgcagac ccgtagtcca ctgaggcggg
     2281 ggcggaggaa tcggtggcac ggggtccggc gagagctcag ccaaaggccg cggctccgcg
     2341 tcatccagat cgctgtccgt ccaagactct ggagcttcga gcaaagccat atgttccgct
     2401 ccgacggaca agtcatagct acgaaagccc aattggccct gctttcgaac caccatcttc
     2461 ttctgccccg cggcgtcgag aattacgaag cgcccgtcgc gcgccaccag cacgacatct
     2521 ccactatcga agattgcagc ggacacgtaa gcgtccttga aaacatatcc ggcatctccg
     2581 tacccctccg gatctggagt atccacgtcg ctcacgatcg cgcgacgtac cacgagcacc
     2641 gttgcgttga tgccctcatg ccgattcgtt ggatactgcg atacgaggat ggcatggccg
     2701 cgccgcacgc gcccccactc tcggacacta ttgcgaaaca gcagctgctc gttctcctct
     2761 tgaccgctct ggagaacagg agttccgcaa gggtacgcca ctcgctcacc aacgagcgcg
     2821 gcccgaggcc agccgccctc cttcacccaa accactgcgc cgctgcgccg attgaaggcc
     2881 aatgtgcgct cccccacact gaggatctgg tctccgaaaa gcagcagagc ggcgctgaat
     2941 gtcgagcccg attcttgccc atcccccatt cttcccaagc gccgcaattc acgatacttc
     3001 acccgtgagc gggacgcgcg ggtggagtcg acccactgaa tttcatgcgc actcgcggcc
     3061 ggaccgctca tcacgttctt cttcatagcg ccttctggtg cctttacggg ggagtgggcg
     3121 ggcattggag gcgggaccgt ggaggggttc cgggcgctcc gtggtatcgc cgtaggaacc
     3181 cgagccgatt cacgttcatt caccttcttg gcagacttcg cgcatgcacc agatatcatc
     3241 gtcagagctc ccgccaagac tagccagttc cgcccggtca cggcgcgctc tccacggcgc
     3301 gctgaacccc tggtgggtca tgaagcagct gcgagacgct agtggccact cgcgcgaagt
     3361 cagacgcaga gaagcgaacc ttaagacgcc cgtttgcgtc aactggtttc gcagcgagtg
     3421 cgtagcagag tatcgctact ggaaacgcca cgacctctga cggtgtaagc actaagttgc
     3481 cagtatgacg ggagtcgagt agtggatgcg gggactcgcg ctgctcgacg acgtcaatcg
     3541 tcaggtgcgg atcgcgcggc cggcgttccc cgttttgtgc tagctcctgc acgctctcga
     3601 tgagcggtga aacactcagc gcaatccgct gcagacatgg ttcagcggtc ggagggcggt
     3661 tggaccgtgc agaacgaact tgcaacatgt agcgtattcg ctccacgtag tctaagtgcc
     3721 cagtctttgg cttcctatat gcaaaacgga cgtccatcag acgagcactg aacacaatcg
     3781 cttcaagaca ttccagtcgc ttcgtttccc caagggaatc agcgaaattg aaaagaatgc
     3841 gcgtatacac cagcgctatt ccgtcatagc gttggtttgg cggaagcgca gtgtcgcgcg
     3901 ttccgcccgg accgaggaac atcttacgga cggtcccttg gtcctgtggc aagcgataaa
     3961 cggcgatgtc cgcgcggttg gtcgcgtgca gagggcgcgg tcccgccacc gggcgatacc
     4021 aggcgtggtt ggcctcatcg ggtagtacgc tcggccccac gaacgggagc tccgaactca
     4081 tcaaggtgcc cggaaccgtc tgcgtgtcga tcgccacata atccttacca tcgagccatt
     4141 ctcgaaactt tgcctcttct cgcagccgat tggcgtagat ccaatagtgg cgcaggcgtg
     4201 gatgactatt ggagttcatg atggacggtt cgtcggtggc aaaaggacgc gcgatgacgc
     4261 cttcaatcgc ctgctgcagt ccgaggaagc ggccccgcaa tgcgctgaac gaggccagtc
     4321 cggtcatgag ctcatcgtac tcgtccggcc agcccagctt atgaccgagt tcgtgcgcga
     4381 gagtaaaacg cgcaaaggcg ggattttggc gccgctcgtc atcgaggtgg gcttggaact
     4441 gtgtgcggga ttcgtcgata tccccatcga acaccgtgat ttccggagta tagtgcccgg
     4501 cggatgcgcg ccccccggca ggtctcacat gaaccacgac cgcgtcggcg cgggaggggc
     4561 cttccccaaa gtagtgacgg actctcacga ccttatcccc ggcggcagca cccgtgagcg
     4621 ggacgaggcc catcgtacaa ggcacgcgat caggcggcgg cgcgttggga tgccccggat
     4681 gcttctggtc ccagcgctct gcagcatggg taagagccgg caacatacgg tccagttccg
     4741 cgccgctcac ccggctgccg tcgagccgta aggggatgtg cacgagcagg tgcatcaact
     4801 tcatgttcac ggcgccgctg gtagtggccg ggaactcgtg gtccatgaat gatgcgtcga
     4861 tcaggtggta ttcggccgaa ccgaacacga cggtctgatt gggtatgccg tgcatatagt
     4921 ccgcgagccg ccgcccggtc gagtcatcga gcaaggctac ccgtgcaccg acacaatcgg
     4981 tatgtccgag cagcgactcc gacttgatgg tcattcggcg ctcgtgcagg tcgtggaaac
     5041 gcccgtcgtg atagacgagg cgcgtgcgcc gctcgatccc ctgcccctcc tgcaccacgg
     5101 ccgtctcggc cgggaagtag ttcttctgcg gcaaggtacg ccaaacatgc gggcgcgcgg
     5161 gatcgggatc tcgaatgtgc agccagtggt cgaatagcgc cacgcgcgat ttgccgttca
     5221 gttgcagcgc aaactcattc tcgtctgcaa gcaccatgtc atcgaggtgg aagcacagcg
     5281 ggctcgcccg ctcgacacct cgggtccgga gagcctcgaa ctctcgacgc tcgacgggct
     5341 gcccatccgg ggccccgata aatgccaaca taccatgaga gtgccattct cgcggcaagt
     5401 agtaatgcga agcggggcgc cgcacgccgc cgtttgcgag tagcgcgttg tcgggcacgg
     5461 ccgcgcccgc tttgacctgg acgcgatgcg caggcgcggc atcggcgtcg agtacgccct
     5521 ggccatcctg acctcgctcc acggtcgcga agtagtagtg cacgtcacca gaatcttgct
     5581 ccgtgcgctc gtgcagtagg tacacgttgc cagaatcatc gatgggtgtt ccggcggcca
     5641 cccgacgttg gagcacggac ttctgatcgg tactcggcgg ctcgtgcccg gggctcagtc
     5701 gagggcgccc gagagcttcg accactacat ggctcgggac agcagctcgc ttcttattgg
     5761 cgacgtcata atagtggaag cgaacgaagt ttcggaacag cccgtgatcc acttgcacga
     5821 cccaaggctt cacgagggtg ccgtgggcaa cgctctcccc ccgagccatc gagaatacgc
     5881 cggtctccgc aaatcgcgtt ttgggcaccc cttcgaggaa tccacccacg aggaccttaa
     5941 tcgaactcga ccagaccggc agacggatca agcggcgggc gtctagacgc cccacctcac
     6001 gcgggagcac ccactcttgc ttatcgaggt cgagaaacac ctcgccggac cgctgtacgt
     6061 cgagagccgg ccgaagacgc ccggccgtgt tgcgaaacaa cggtctccac gcgagttccc
     6121 actctgcaaa cggatcgaca ccgtcgaatc tcaggtcgtc ttcgaattcg atccacgaga
     6181 tcccatcctt gttgcttggt cccccaaaga tgggcgctag cgatcctggt ttcagattca
     6241 acagaccaaa ctgatgatcg ggcagaggca cgtcttgccc acgcctcgga tcgaagaact
     6301 gtccccgaat cacgaagcgc gccgccgcca aaggaaccac cagccccgcc gtagtccggg
     6361 taaccggacc cgtgggcatc agcgccggaa cgtcatttcc actcattcga cccaccaacg
     6421 ccaaagacca aacacccggg cacacaccta ggtccgtcga gaccgcctac cgcggtgcgc
     6481 tcgagattct tcctttcacg cgcttccgat ctccaagcga gccccgctag caattgaccg
     6541 cggcaaacca cccgagcaag gacgcaaagg aacttgctcc acccggaacc tccaattagt
     6601 aaacccgtgc atgggttagc gcatcgctcg cagagctgtc aagccgaagc gcgccccgag
     6661 gaagccggac cccgattcgc acgaagacgc ttcgctcgtt gcttctatgc accgcccacg
     6721 gttactcgta gatgttgcct acgtagaccg gatgaccatg ttcgggagtg gccctgacct
     6781 gcggcgcggc gcgcggcgta aattccgcta acatgggtcg tgggcccgcg acgggcgagg
     6841 cctcgaacac caattgggat cgaaggatgc aagtggatat cgtcccctcg ggggtggtca
     6901 gtctggagat gatgcgggac ctcggggagc tgcaggaaga gctcacggaa gcggggctcg
     6961 acgcgcgcgg ctcacacgct tcgatccccg gagtcaaaga cggtggattg acgatcgcga
     7021 tcggcatcgc tggcgtcgtg ctatctgcgg tgtcttccct ggtatccgta ctcacctact
     7081 ggagctccag aaagccgagt tactcggtga aggtggaggc cggctcgggc acactcacga
     7141 tctcggatct cgatccttcg agcgtacagg acgtgttgaa gcgcctgaaa gaagtcgatc
     7201 ccaagagcgg cttgctggtc cgcgtcactc gacgttgatc gcgccatggg tcgtcggctc
     7261 gccttggtag tgagtgcgga ctccggctct gaactgagcg ggggcaagcg cgacgccatg
     7321 cgcgttttct cggtgctcgc cgacgagaat ctgggcgcgt gtgatccaga gagccctcag
     7381 cccatcgcgg cctgcagttc ccgcgctcgc tttcacgaag cctggcactc actcctgacg
     7441 aactggaacc cccaggacca actgctgttc tacttctctg gacacggggt gatgaagcac
     7501 ggccggtaca cgctcgcctt tggcgccgcg ccctcccagc actatgtacc tttcgatcat
     7561 ctgacctcgg atcttcaagc ccacggcgta cgccgcgccg tggtcattct ggatgcctgt
     7621 cacagtggtg ccgcgctgca agcaggagcg aaaggaacgg acggggagtt gcccatccaa
     7681 acgctccccg ccgggatcgt ggtgttgacc tcttgcagaa gcgcggagca aagcttcgaa
     7741 ttcgaggatg gcgcaggcag cgtgttcacg cacctgctca ctcaaggcat ccgttccggg
     7801 ctcgggggca agccgacccc ggacggcttg atcggaccgg acgatctcgt cgaatacatc
     7861 aacacgagac ttgccgagcc ggagtacgcc aattatccgc agcggcccag ctatcaggtg
     7921 tcgtccgcgg atcgcacggt gtggatcgcc cgcaataggt cggtcgcgcc cgcagaggca
     7981 agacccaagg tcgccacgcg agcctattcc atcgagcagc tgcatctact ctacgataga
     8041 accgagaaga ctcggcaccc ttgtccaggc gccacgttgg attgtctgga ttgggatcta
     8101 gtcgcacagt atgcggaagc ttcacgcacc ccgctcgagt cgacagagtc gacagagtcg
     8161 agagaccaag cggcacgcag gttgggactc tattcgagcc tatccgatga cgtactccat
     8221 aacgcggccg tgctgtgttt tgcgcgagcg ccgcatgctt tgctgcagca agggcgagca
     8281 ctgtatctgc gcggcgaacg gagcagcact catgcactgc gaacagatgt gctcggccca
     8341 ctgtcacagc aagtgcgcga gttgactcag ctggtaatga ccgacgtata tcggcgctgg
     8401 ggttggcgtc cgacggatcc cacggcgacg ctgctggccg aaacggtgcg cgaggccgta
     8461 tcgaacgcgg ttgcccaccg cgactacgac cgcagcgaac tcgtacgtgt ctcgatgagg
     8521 gatgattgca tcgagatcac gaacccgggt gcgttcccgc gcggcacgac cttcgagtca
     8581 ctgctcgccg ccggcaacta ctccgagccc aacgacgccg cgatagcctg gtatctgacg
     8641 acgttgctgg cgtacgaggg cgtggggcgc ggattctcgg tctacaacgc cttccgcgct
     8701 gcggccgggg ataacgcgct ggagtgccac gtccaggctg agcttcgatc gattcggctc
     8761 gtgctgcgga ttcccaccca gattgctccg cagctcgcgc cggtgccaga gaagacggag
     8821 ccgccggccg cggtcccgac tggaccgggc acacttcacg gtctggggcg ggatgtggtt
     8881 tttggagaac cgattcactc gacggttgca cagcccaatc tgatcccgag gacgggcacc
     8941 gaagaactcg ttgggcagcg ttatcggttg gggcctacca tcggcacagg cgcgtctggt
     9001 accgtgtttc tggcagaaga tacccggctg gcgcggcagg ttgccgtcaa ggtgctttca
     9061 aagcgtttcg tcacggccga agtgcgcgag cggttcctgc aggaggcgaa gctcgccgcg
     9121 cgcgtggttc atccgaacgt ggtcgccgtg tacgacgtgg gagaagagac cgatcgcctg
     9181 tacatagtca tggagctcgt caccggtgac aacttggacg cgaccattcg cggttctgaa
     9241 cgtctgcgcg agcgggaacc cgatctcttc tttctacaag cggcgtggtt gatatacgac
     9301 ctggcggcgg ccctggccgc ggcacacgct agcttgatca tccatcgaga catcaagcct
     9361 tccaacgtga tcgtggaaca gagcggctcc ccgcgtcttc tcgattttgg cgtctcccgc
     9421 gcggccagcc gggacaccga cagtcgcttg accgccaccg gcgtgctcgt cggaacgcca
     9481 tactatatga gcccggagca ggtgcgtggg gagatcgagg acgaacgctc ggacatctac
     9541 agcctcggcg tggtgatgta cgagctgctg tgcggccggt tgccgttcga agcgcaggcg
     9601 ctgccaagcc tctttatcga catcactcag ggcgacttgg tgcagccccg ccgccatgct
     9661 ccacgaattc ccgtcgaact cgagagtatt tgtcttcgag ccatgcagcg cgagcgcgta
     9721 cgccgctacc ctcaggcgga ttcgatggcg ctagagcttc gtcagtacct gccaagcaat
     9781 ccgtgccacc gcctctattc gcaggatcgc gccaagttat gggccgattt caaggctcgt
     9841 ctcgacaagg cttagacgcg gcgcaagcgc tgaccggagc tgcgtgcgct ccggagcggt
     9901 taaagaatcg ccgcccaagc cgggatcgtg atgagtgcca atacgaggcc ccaaccaacg
     9961 aaggcggccg cgagttcacc ggccacccct tgagccatca tgagcgcgcc cgcggtgatc
    10021 atggtgggca tggcggtctc gagcacgttg accttcaaga ctagcggtgg cgcgtgcaga
    10081 agcgcggata taccccaagc cagcagcggg aagattgcca atttgagcat gagccccaga
    10141 gcgaatacac gcctcggacg cggtgcactg aaacgcaact tgaggccaac cgcgaacatc
    10201 gcgagcggta cgagcggagc ggccgccccg atcagcaata catcgagcca agccatgtgc
    10261 ggcacatgca aagcagcgag cagcagagcc agcagcagcg cgaggaacgg cggaaattgg
    10321 agaactcggc gcagaagctc gctccacttc ggcgcgctcc ccgccgtggc ccgagcgagc
    10381 gcgaccggcg ccaccgtacc cagcagtagg aaggagccga gttgatcgta gacggccgcg
    10441 agcggcaccg cggcttcccc gagcaacgcc tgacacagcg gaaaccccag aaacgaagta
    10501 ttgccgagcg aggtagccaa aaacaacgcg atcgtgtgct cacgttggag cctcagtagt
    10561 ttttgcgcaa ctcgcgcgac ggcccaggcc agccccgcga agaaccaggg ggtgatcgca
    10621 agcaaaagga ggtgcgaacc caacgtgagc ttgggaacca accgcaggat cgtggccggc
    10681 acacagatgt cgatgacgaa ccgattcagc acatcggccg cgttgtcagg caggcgattg
    10741 aagcgacgcg cgcagtagcc cgcaacgaac atgaagcaaa ccaaggctag ggcggaagcc
    10801 atccggggcg c
//
LOCUS       DASTKU010000265        12390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1613893, whole genome shotgun sequence.
ACCESSION   DASTKU010000265 DASTKU010000000
VERSION     DASTKU010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12390
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613893"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1253
                     /locus_tag="VFQ61_09580"
     CDS             252..1253
                     /locus_tag="VFQ61_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9954746.1"
                     /translation="MRRKKQRDDKIIHVVFGPGGGRIQRRPESESGSEATSENNGSSR
                     EPLTDLFTLTEIARLLDFSPTRLRTLDRAGVVSPSGRRRGRRAYTFSDLIALRTAKTL
                     LERKVRLRDVTRAIQALKHSLPRVTRPLQELRIVSDGQRVVVRTQEGSFEPLTGQMLL
                     DLEVKSLREDVVRVLRPSAGRERARTAYELYLRASQLDEDPRTMDEAERLYLEAVKLD
                     PWLAIAYTNLGNIRFRRQDTDGAEALYRKGLEIDPRQPEAQYNLGYVMLERGQPDAAI
                     PLFLGAIASDPKFSDAYFNLAMAYEQVGETQKARPYWKNYITLEPSGTWTEIAKRHL"
     gene            complement(1292..2149)
                     /locus_tag="VFQ61_09585"
     CDS             complement(1292..2149)
                     /locus_tag="VFQ61_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET9954747.1"
                     /translation="MPHALASLKVAALQLSSQASPAENLGKVSNLVRAAARAGAGFVV
                     LPENFAFMGTDGAKSAVAERLGDDRGPIQAALRELCVTERVSILAGGFPELSPDAQRP
                     FNTSALFDERGELVCAYRKLHLFDVDLGTHGALSESRTTSAGQEIVVAPVQGFQVGLS
                     ICYDLRFPELYRALVDRGAEILTVPAAFTLYTGKDHWHVLVRARAIESQCYVIAAAQW
                     GKHPEGRMTYGHALIADPWGTVIAECSDHEGFALATVERSFIQEVRSRIPCLSHRRLP
                     LSVPKDPVL"
     gene            2217..3599
                     /locus_tag="VFQ61_09590"
     CDS             2217..3599
                     /locus_tag="VFQ61_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9954748.1"
                     /translation="MSFTTGAMQEFGAAGRAGRSLSGLVLLFAETFGALPPAFPFTRD
                     RLVLGREPPADIVLPANAVSRVHAEVTWQGGHYVITDLNSRNGVLVNGRRVGQAALEP
                     LDEVRIGDVLFKYVDQNAEFYALYRIDGSMIPSARRYAQNPTALVGGFQMDCMVTELE
                     RIAPSELNVLLLGPSGTGKEVVASELHRLSARPGPFTAVNCAAIPANLIESELFGFKR
                     GAFSGADRDKPGIIRAAHRGTLLLDEIGDMPLDAQAKLLRVIQQKEVLPLGATQVERV
                     DVRIVSATHRDLSRMQREGRFREDLFARLNEYKVRLPPLHERKEDIYALVRHFLRSLG
                     RPDLMAGVPFMVALLHHDYPYNVRELEAAVKRAAALARGPQLEVDLLPDAIREALSDY
                     GRAALAANDRSEVAAEAFAPVPSEEELRRRLTAHGGNVAAVGRELGKARMQIHRWMKR
                     YGIEVDDYRG"
     gene            complement(3642..3911)
                     /locus_tag="VFQ61_09595"
     CDS             complement(3642..3911)
                     /locus_tag="VFQ61_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9954749.1"
                     /translation="MPEQRRSVQELESLGKRLGGIAVGEVLPGSAADLAGVELGDVVL
                     RVNGIATPTFEEFLVVAEAHLAHLEFEVFRDGHLLRLSDDPPASS"
     gene            4278..6614
                     /locus_tag="VFQ61_09600"
     CDS             4278..6614
                     /locus_tag="VFQ61_09600"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing)"
                     /protein_id="HET9954750.1"
                     /translation="MEPPTQEEPAALHEIAQSRYLNYALSVITARALPDVRDGLKPVQ
                     RRILYTMWQQNITADTKHRKCAKVVGDVMGNYHPHGDAAIYDALVRMAQPFAMRSPLV
                     DGSGNFGSMDGDPPAAMRYTECRLAGLSARLLEELEQDTVPFRPNYDGTKTEPVVLPA
                     RLPNLLVNGTTGIAVGMATNIPPHNPEEVCQASIRLLDALLEGKQLSSRELCRTIKGP
                     DFPTGGQLISTTEEIKQAYESGQGTFKVRGTWKEAGTTRNGKLVHITSIPYAVNKATL
                     VERIAEVVVSRAMPLIIDVRDVSTSDVCIELELKKEADEQKVLAYLYKHTPLQSNFAL
                     NMTCLVPTENPEVGRPERLDLSQILWHFLHFRLEVVTRRLAHELAALKKRIHILQGFE
                     KVFDALDQIIKIIRASEGKADAATKIMARFQLDAEQTDAILELKLYRLARLEILVIQK
                     ELKEKRKREKEIRTLLDERESRGRWAIVRGELVEIGEAFGKDGKRRTTIETSGSEVEF
                     SEEDLIVAEDNYVLLTVDGWVKRQKEIKDTATTRLREGDAVLACEIGSTRASMVFFSN
                     FGTAYTTRIIDIIATTGYGEPIQKLFKMKDGERIIAMYSLDARVRDASEPKHGKPRCH
                     AVAATSDGNALCFGLSAYLEPSTRAGRRFAKPSEGVEVVGVELGSGAETLIAATRDRR
                     ALLCRMDEIAYLSGPGKGVQLVKLEDEDVLIGFKLARSSDDALVVETSLGGEHKIAVN
                     KYELSSRGGKGREVTKRGTLVRVVRPLPPAPAPLEGAS"
     gene            6701..8611
                     /locus_tag="VFQ61_09605"
     CDS             6701..8611
                     /locus_tag="VFQ61_09605"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET9954751.1"
                     /translation="MQQRTYTAKDITVLEGLEPVRKRPGMYIGGVGSAGLHHLVWEIV
                     DNAVDEAMNGHASDIHVTLHKDGQSVTVSDNGRGIPVDVHPTHKKSALELIFSTLHAG
                     GKFDNQNYKTSGGLHGVGASVVNALSSKLIATVRSDGAEYKMEFRAGTPVSKLTKVGA
                     ARGSGTQVFFRPDPTIFPKIEFSADVIRDRLEIISYLHRGLKVHFVDEVNAQKHDFYH
                     ENGLVDYLGKLTALSGKSPVHDAPFVLSKEGDGRLEVALRWTESTEEVVKSYVNGIFT
                     GTGGTHENGFRTGLVKAVRNYIETHNLSPRGVTLAAEDIREGLYGIISVFVTEPQFQG
                     QTKDRLNNPEVQSQVDSAVRPALEQWLNQNRTMAEQIVARIILAARAREASRAASAQI
                     SRKSATSGRLTLPGKLSDCLSHDRSHTELFIVEGDSAGGSAKQGRDRNRQAILPLRGK
                     VLNTEGLALGKVLENKEIADLITALGCGVGANFDLGKLRYQRVVLLADADSDGHHITT
                     LLLTFLYRHLPELIRQGRVFVAMPPLYRIDIGKETYWAADDPDRDKILKEKQKGNAKA
                     DITRFKGLGEMMPKVLWDTTLNPKTRRLLRVEIQDALETDRIVNELMGKDASARFRFI
                     MDRADQAEELDV"
     gene            8691..11465
                     /gene="bamA"
                     /locus_tag="VFQ61_09610"
     CDS             8691..11465
                     /gene="bamA"
                     /locus_tag="VFQ61_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HET9954752.1"
                     /translation="MKNFPHGLLSAWQNLGTGLTRGGRVRQKLVSHSPLVPSLRKLGA
                     RPRSIATCLALLVMSTSTLAGAQAQPAAPPTPAVTKPAAKRPVTARPAPPKPVAAPND
                     GEEAAADPSAEPAPADATGESRPERPRVEIPPNAAEQAAGLPITAIQIAGNRRVTQDD
                     IRAYLRERVGQPFSPPALTQDVRELYAAGFFDDIEVDLDRTDTGVTLRFLVRERPSVS
                     SIEFDGNEEIDDDALKEGIELKAETVLSQPAVRRSVQKIRDLYAEKGYFLAQVESEVL
                     PQKNNEVRVVFKVKEQQRVSVRRITFIGNDHIPSDELRALMYTGNAGFFAFGSGGPFR
                     QDAFERDIAVISASYYDRGFLSVSINTPRVMLTPDKNGIEVAITIDEGPRFKIRQLRV
                     FERGADGREVEPIDGRRNLRMMVRAETGDYFNRAALLEDLQTIRTLYRDHGYANVDAT
                     PVTNVNPQTNEVDVIVPVERGPLVHFERIEVRGNSKTRDRVIRREMEIAEGQEFSESL
                     LERSRRRITALGYFERVDVSTEQGSAPDKMNVYVEVTEKPTGTFQIGAGFSSLENFIA
                     TAQVQQANLFGNGQSLSLQAQVSGIRQLVDIRLYEPYFLKSKFSASINLFSQLRVYTD
                     FSQTSTGGSLTFGYPLLEPELTASLTYTGQVDRVSTQAVSTWFGSGTGTATSVFARLP
                     LANLFNDGFTSSIRPGLVYDTRDNRLFPTSGIYLSASTEFATSALGSDNLFLRHRLLG
                     RFYYPLGGGFVLKLNSEAGHVTSPSSDGVPIFARFFLGGIFDVRGFRYRTLGPRVPLT
                     GNTDPNSLPIPNGANIGGNLMYFQNLELEIPVLESVGVRGVLFTDAGNAWNLEQNYCG
                     ANPGGQIYPETSPCFDGLKSLARLRTSWGFGIRWFSPLGPLRFEWGYPFKTLPYEEKH
                     VFEFTIGNFF"
     gene            11588..11947
                     /locus_tag="VFQ61_09615"
     CDS             11588..11947
                     /locus_tag="VFQ61_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954753.1"
                     /translation="MAAADPREAPTTDRTPGLTTTGAAASGPREVGREASRASLSPNS
                     DTLLSAPTPATREDFAFDERLSEAERRVAELLLRVEHLERAAGRPSQNRPPRALWFWF
                     VFLAGLALAWQILSRFR"
     gene            12073..>12390
                     /locus_tag="VFQ61_09620"
     CDS             12073..>12390
                     /locus_tag="VFQ61_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954754.1"
                     /translation="MLPLVGIIGWRGMVGSVLVERMLAEKDFDVIEPRFFSTSQAGGK
                     APNIGRDVPAVGDAKDLKALAQNEILISCQGGDYTKEIHGALRASGWKGYWIDAASAL
                     RMSD"
BASE COUNT         2418 a         3940 c         3968 g         2064 t
ORIGIN      
        1 ccgcgcgaat gcaagccctg agcgcgctct cgtaaacatg atccgggcag gattcgaaag
       61 aacgatcggg acacgatcag gaacacgtca ggaacaggat caggatcagg atcaggaaca
      121 catgcgcagc gattttatcc agagacgatc ggccggtccg aaagagtgag ctacccctct
      181 tgacagggga tcgtacgcag aagaattgaa agagcctctg gctcgcagta ctcaagcgat
      241 cgcggttggc aatgcgtcga aagaaacagc gggacgacaa gatcattcac gtcgtgttcg
      301 gacctggagg cggacgcatt cagcgccgcc ccgaatccga aagtggaagc gaagcgacgt
      361 ccgaaaacaa cggcagctcg cgtgagccac tcaccgattt atttacgctg acggagatcg
      421 cgcggctgtt ggatttctca cccactcggt tgcgcacatt ggatcgagct ggcgtcgtat
      481 ctccttcggg ccggcggcgc ggccgtcgcg cgtacacctt ttcggatttg attgcgctgc
      541 gcaccgccaa gacgttgctg gaacgtaagg tgcggctgcg cgatgtaacc cgggccatcc
      601 aagcgctcaa acactccctg ccgcgagtca cccggccgct gcaagagctg cgcatcgtct
      661 cggatgggca acgcgtcgtg gttcgtacgc aggaaggtag cttcgaaccg ctcaccgggc
      721 agatgctttt ggatctcgag gtgaagagct tgcgtgaaga cgtggtgcgc gtcctgcgcc
      781 cttccgcggg ccgagagcgc gcccgcaccg cttacgagct gtatctgcgc gcgagccagc
      841 tggatgaaga tccacgcacc atggacgagg ccgagcgcct ctatttggag gcggtcaagc
      901 tcgatccatg gctcgcgatt gcgtacacga atctcggcaa catccgcttt cgtcgccaag
      961 acaccgacgg cgcggaggcc ctctaccgca agggactcga aatcgatccg cgtcaacccg
     1021 aggcacagta caacctgggt tacgtgatgc tcgaacgagg ccagcctgac gcagccattc
     1081 cgttgttctt gggcgcaatc gcttccgatc ccaagttctc ggacgcgtac ttcaacttgg
     1141 ccatggccta cgagcaggtc ggcgagaccc agaaggcacg gccgtattgg aagaactaca
     1201 tcacgctaga gccgtccggt acctggaccg aaattgccaa gcggcacctg tagtcgccgg
     1261 gcggcaccgg cataacgcga acaggcccgc ttcagagaac tggatctttg ggcaccgaga
     1321 gcggcaggcg acggtgggag agacacggga tccggctgcg cacttcctga atgaaggagc
     1381 gctccaccgt ggcgagggca aagccttcgt ggtctgaaca ctccgcaatc acggtgcccc
     1441 agggatcggc gattaacgca tgaccgtagg tcatgcgacc ttcaggatgc ttgccccact
     1501 gggcggccgc gatcacgtag cactgagatt cgatggcgcg cgcgcgaacc agcacgtgcc
     1561 aatggtcttt gcccgtatac agcgtgaagg cggcgggcac cgtcaggatc tcggcgccgc
     1621 gatccacgag cgcgcggtac agctctggaa agcggagatc gtaacaaatc gagaggccta
     1681 cctgaaatcc ctgcacgggc gcgaccacga tctcctgccc cgccgaggtc gtgcgcgact
     1741 cggagagcgc tccgtgcgtc cctaaatcta cgtcgaacag gtgcagcttg cggtacgcac
     1801 acacgagctc accgcgctcg tcgaaaagcg cgctggtgtt aaaggggcgc tgtgcatcgg
     1861 ggctgagctc ggggaagccg cccgccagga tcgagacgcg ctctgtgaca cacagctcac
     1921 gcagcgccgc ctggatcgga ccgcgatcgt ccccgagccg ctcggcgacc gcgctcttcg
     1981 caccgtccgt gcccatgaac gcgaagttct ccggcaaaac cacgaatcct gcgccagccc
     2041 gagccgccgc ccgcaccaga ttcgaaactt ttcccaagtt ctcggcggga gaggcctgac
     2101 tagagagctg gagcgcggcg acttttagtg aagcgagggc gtggggcatg ggctgggaca
     2161 taggcagagt gggattgagc ctgggcaact agaagcgcca agtttatgct gagagagtga
     2221 gttttacgac cggtgcgatg caggaattcg gtgccgcagg gcgagcgggg cgctcgttgt
     2281 ctgggctggt gctcctgttc gcggaaacat ttggtgcttt gccccccgcg ttcccattca
     2341 cccgggaccg cctcgtgctc ggtcgggagc cgccggcgga tatcgtgctg cctgccaacg
     2401 cggtctcgag ggtccacgcc gaggtgacat ggcaaggggg tcattacgtc atcacggacc
     2461 tgaacagccg caatggcgtg ttggtcaacg ggcgccgcgt cggtcaggcc gccctcgagc
     2521 ccctcgacga agtgcgtatc ggcgacgtgt tgttcaagta tgtggaccag aacgccgagt
     2581 tttacgcgct gtaccgcatc gacggcagca tgatcccgag cgcgcgccgc tacgcgcaga
     2641 atccgaccgc gctggtgggc ggcttccaaa tggactgcat ggtgaccgag ctcgagagga
     2701 ttgcgccctc ggagctcaac gtgctgctgc tagggccgag cggtacgggc aaagaggtgg
     2761 tcgcgagcga attgcatcga ctgagcgcgc gcccaggccc gtttacggct gtaaattgcg
     2821 ccgccatccc cgccaacctg atcgaaagcg agctgttcgg cttcaagcgg ggtgcattct
     2881 ccggggcgga tcgtgacaag ccgggcatca tccgagccgc tcaccgaggc acgctgctgc
     2941 tcgatgagat cggagacatg ccgctcgacg ctcaagccaa gctgctgcgc gtcattcagc
     3001 aaaaggaagt gctgcctttg ggcgcgaccc aggtcgagcg tgtagacgtg cgcatcgtct
     3061 ccgcgacgca ccgagatttg tcgcgcatgc aacgcgaggg gcgctttcgg gaggacctat
     3121 tcgctcgctt gaacgagtac aaggtgcggc tcccgccgct gcacgagcgc aaagaggata
     3181 tctacgcgct cgttcgccac ttcctgcgta gcctggggcg accggatttg atggcgggcg
     3241 tgccgttcat ggtcgctctg ctccaccacg actatccgta caatgttcgc gagctggagg
     3301 cagccgtgaa gcgagccgca gcgctcgcac gggggcccca actcgaggtg gatttactgc
     3361 ctgacgcgat ccgagaagca ctgagcgact acggtcgcgc ggccctggcc gcgaacgatc
     3421 gcagcgaggt cgcggcggaa gctttcgcgc ccgtacccag cgaggaggag ctgcgtcgac
     3481 gcctgacggc gcatggcgga aacgtcgccg ccgttggacg cgagctcggt aaggctcgga
     3541 tgcagatcca tcgctggatg aagcgctatg ggatcgaagt cgacgactac cgcggttgaa
     3601 tcggtaggtc gctccctatc gaaggcctgc tctgagccat ttcacgaaga cgctggcgga
     3661 tcgtcgctga ggcggagcaa gtgaccgtcc cggaacacct cgaattcgag atgagccagg
     3721 tgggcttcag ccacgaccaa aaactcttcg aatgtgggag tcgcgatacc gttgacccgc
     3781 aacacgacgt cgcccagttc gacccccgcg agatctgcgg cgctaccggg caggacctcg
     3841 ccaacggcga tgccccccaa acgtttgcct aaggactcga gctcctgaac gctgcgacgt
     3901 tgctctggca ttgggatgga gggcggtttt ccatgaattg tgccagcgcc tagaggccgc
     3961 aaacccgcag aaattcagcg ggtaacgtcc agttccgggg cgggatccgt ctcgctctgg
     4021 tcccgtcgcg tctcgacctg ctccaactgt gacgggagca catcggcgga agcggctcgc
     4081 gggcggaagc ggccggcctg actgcccaat ggttgacttc ctgcggcgaa agacggtagc
     4141 gaaacgcggc cggtgcgcgc ttcgagtcgc cgcgccgggg agttgccaaa gctttgggta
     4201 cccgaaagaa aaccagtcag ggttcgaacg aagccacgcc cccgcggcgc ggaaaaggcg
     4261 gaaatcaagg tccgcccgtg gagccgccga cgcaggaaga gcccgcggcg ctacacgaga
     4321 tcgctcaaag ccgatacctg aactacgcgc tcagcgtgat cacggcgcgc gctctgcccg
     4381 atgtgcgcga cggcctgaag cccgtgcagc ggcgcatcct gtacacgatg tggcagcaga
     4441 acatcacggc cgacaccaag caccgtaagt gcgcgaaggt ggtgggcgac gtgatgggca
     4501 actatcaccc ccacggcgat gcggcgatct acgacgccct ggtgcgcatg gcgcaaccgt
     4561 tcgcgatgcg cagcccgttg gtggacggct ccgggaattt cggctcgatg gacggcgatc
     4621 cgccagcggc catgcgttac acggagtgcc ggctagcggg gctctcggcg cgcctgctgg
     4681 aggaactcga gcaggatacg gtgccatttc gcccgaacta cgacggcacc aagaccgaac
     4741 ccgtggtgct gcccgcgcgc ttgccgaacc tgctcgtgaa cggcacgacc ggcatcgccg
     4801 tgggtatggc gaccaacatt ccaccgcata accccgagga ggtgtgtcag gcatcgatcc
     4861 gcctgctcga cgcgctgctc gagggcaagc agctctcttc tcgagagctt tgccgcacga
     4921 tcaagggccc cgatttcccg accggagggc agctgatctc gaccacggag gagatcaagc
     4981 aagcttacga atccgggcaa ggcaccttca aggtgcgcgg cacttggaag gaagccggca
     5041 ccacgcgcaa cggcaagttg gtccacatca ccagcattcc gtacgccgtg aacaaggcca
     5101 cgctggtcga gcgcatcgcg gaggtcgtgg tgtctcgcgc gatgcccttg atcatcgacg
     5161 tccgcgacgt ctctacgagc gatgtctgta tcgagctcga gctgaagaag gaagccgacg
     5221 agcagaaggt actcgcttac ctctacaagc acactccgct ccaatcgaat ttcgcgctga
     5281 atatgacctg cctggtgccc accgagaatc cagaggtggg ccggcccgag cgcctcgatt
     5341 tgtcccaaat cttgtggcac ttcctgcatt tccggctcga ggtggtaacg cggcgcctag
     5401 cgcacgagct cgccgcgctc aagaagcgca tccacatcct gcagggcttc gagaaggttt
     5461 tcgacgccct cgaccagatc atcaagatca tccgggcctc cgaaggcaag gcggacgcgg
     5521 ccaccaagat catggctcgc ttccagctcg acgccgagca gaccgacgcg atcttggagc
     5581 tgaagctgta tcgcttggca cggctcgaaa ttctcgtcat ccagaaggag ctcaaagaga
     5641 agcgcaagcg cgagaaggaa atccggacgc tgctcgacga gcgcgagtcg cgcgggcgtt
     5701 gggccatcgt gcgcggcgag ctggtggaga ttggcgaggc gttcggcaaa gacggcaagc
     5761 gccggaccac gatcgagacc tcgggcagcg aagtcgagtt ctccgaagag gatttgatcg
     5821 tcgcggagga caactacgtg ctcctaaccg tcgacggttg ggtcaagcgc cagaaggaga
     5881 tcaaagacac cgcgaccacg cgcttgcgcg agggcgacgc ggtgctggcg tgcgagatcg
     5941 gctcgacccg cgccagcatg gtctttttct ccaacttcgg aaccgcctac acgacacgca
     6001 tcatcgacat catcgcgacg actggctacg gcgagccgat ccagaagttg ttcaagatga
     6061 aagacggcga gcgcatcatc gccatgtatt cgttggatgc tcgcgtccgc gacgcctccg
     6121 aaccgaaaca cggcaagccg cgctgccatg ccgtcgccgc gaccagcgat ggcaacgcgc
     6181 tgtgcttcgg actgtccgct tacttggagc ccagcacgcg cgcgggccgg cgctttgcca
     6241 agccgagtga gggggtagaa gtggtcggcg tcgagctcgg gtcgggcgcg gagacgctga
     6301 tcgcggcgac gcgcgatcgg cgcgccctgc tctgccgcat ggacgagatc gcttatctct
     6361 ccggcccggg caagggcgtc cagctcgtga agctcgagga tgaagacgtg ctgattggct
     6421 tcaagctcgc gcgaagctcc gatgacgcac tcgtggtcga gacgtctttg ggcggtgagc
     6481 acaagatcgc cgtcaacaag tacgagctgt cctcgcgcgg cggcaagggc cgcgaggtca
     6541 ccaagcgcgg caccttggtg cgcgtcgttc gcccgctacc ccccgcgccc gcgccgctcg
     6601 aaggggcgag ctgaaagcgg ctcagtcctg agtgacctga accccggctc gctcccctcg
     6661 ccctccaccc tccgcaattt cgactagacc taggattcgc atgcagcaac ggacgtacac
     6721 ggcaaaggac atcacggttc tcgaaggcct cgagcccgtg cgcaagcgcc ctggcatgta
     6781 catcggtgga gtgggctcgg ctggccttca ccatctggtt tgggagatcg tcgacaacgc
     6841 cgtcgacgag gccatgaacg gccacgcctc cgatattcac gtcaccttgc acaaggacgg
     6901 ccaaagcgtg acggtgagcg acaacggccg cggtatcccc gtcgacgttc acccgacgca
     6961 caagaagtcg gcgctggagc tgatcttctc gacgcttcac gcgggcggca agttcgataa
     7021 ccagaactac aagacctccg gcggcttgca cggcgtgggc gcgtccgtgg tcaacgcgct
     7081 gtcctccaag ctaatcgcca cggtcaggag cgacggcgcg gaatacaaga tggagtttcg
     7141 cgccggaaca cccgtcagca agctgaccaa ggtcggcgcg gcgcgcggct cgggaaccca
     7201 ggtgttcttc cgccccgacc cgacgatctt cccgaaaatc gagttcagcg cggacgtgat
     7261 ccgagatcgt ctggagatca tcagctacct gcaccgcggg ctgaaggtcc acttcgtaga
     7321 cgaggtcaac gcccagaagc acgacttcta tcacgagaat ggcctcgtcg attacctggg
     7381 caagctcacc gcgctgagcg ggaagagccc cgtgcacgac gcgccgttcg ttttgagcaa
     7441 ggagggcgac ggtcggctcg aggtggcgct gcgctggacc gagtccaccg aagaggtcgt
     7501 gaagagctat gtgaacggca tcttcacggg cacgggcggc acgcacgaaa acggcttccg
     7561 cacgggcctg gtcaaggccg tgcgcaacta catcgagacg cacaacctct cgccacgggg
     7621 cgtcacgctg gccgccgagg acatccgcga gggtctgtac ggaatcatca gcgtgttcgt
     7681 gaccgagccg cagttccagg gtcagaccaa ggaccgactg aacaaccccg aggtgcaatc
     7741 gcaggtggac tcggcggtgc gcccggcgct cgaacagtgg ctgaatcaga accgcaccat
     7801 ggccgagcag atcgtggcgc gcatcatcct agccgcgcgc gcgcgtgagg cctcacgcgc
     7861 ggcgtcggcc cagatctcgc gcaagagcgc gaccagcggt cggctcacgt tgcccggcaa
     7921 gctgagcgac tgcctgagcc acgatcggag tcacaccgag ctcttcatcg tcgagggtga
     7981 ctcggccggc ggctccgcga agcaaggccg cgatcgcaat cgtcaggcca ttttgccgct
     8041 tcgaggcaag gtcttgaata ccgaaggcct cgcgctcggt aaggtgctcg agaacaaaga
     8101 gatcgccgat ctgatcaccg cgctaggctg cggggtgggc gcgaatttcg atctcggcaa
     8161 gctccgctat caacgcgtgg tgctgctggc cgatgccgat tcggacggcc accacatcac
     8221 gactttgctg ctgactttcc tgtatcgaca cctgcccgag ctgattcggc aggggcgcgt
     8281 gttcgtggca atgccgccgc tctaccgcat cgacatcggg aaagagacct actgggccgc
     8341 cgacgacccg gaccgcgaca agattttgaa agagaagcag aagggcaatg ccaaggccga
     8401 catcacgcgc ttcaaaggtt tgggcgagat gatgccgaag gtcctatggg acacgacgct
     8461 caacccgaag acgcgaaggc tgctccgcgt cgagattcag gatgccttgg agacggatcg
     8521 gatcgtgaac gagctcatgg gcaaggacgc ctcggcccgc tttcgtttca tcatggatcg
     8581 cgccgatcaa gcggaagagc tcgacgtgtg attcggactt cgtacgtgat cggacatccg
     8641 ttgaatcccg aaaaacctca cggtcggcca cccgccccac agaggatttc ttgaagaact
     8701 ttcctcacgg gttgctctcg gcttggcaaa accttggcac tgggctaacg cggggcggcc
     8761 gcgtgcgcca gaagctcgtt tcccattcgc ccctcgtccc ctccctaagg aagctagggg
     8821 cgcgaccgcg ctcgattgcg acctgcttgg ctttgctcgt gatgagcacg agcacgctgg
     8881 caggggccca agctcagcct gcggccccgc ccacgccggc ggtgaccaag cccgctgcga
     8941 agcggccggt cacggcgcgt ccagcgccgc ccaagccagt ggcagcgccg aacgacggcg
     9001 aggaagcggc ggcggacccg agcgcggaac ccgcgccagc ggatgccacc ggggaatcgc
     9061 gccccgagcg accgcgggtg gagatcccgc ccaacgcggc cgagcaggcg gcagggctcc
     9121 cgatcacggc catccagatc gcgggcaacc ggcgcgtcac gcaggacgac attcgcgcgt
     9181 acctccggga gcgcgtcgga caaccgttct cgcctccggc actcacgcag gacgtgcgcg
     9241 agctatacgc ggccggcttc ttcgacgaca tcgaagtcga cctcgatcga accgacacgg
     9301 gagtgacgct gcgcttcttg gtgcgggaac gcccgagcgt ctcctcgatc gagttcgacg
     9361 gaaacgaaga gatcgacgac gacgccctga aagagggcat cgagctcaag gccgagacgg
     9421 tgctcagcca accggcggtg cggcggagcg tgcagaagat ccgcgatctc tacgcggaga
     9481 agggctactt cctcgcccag gtcgagagcg aggttctgcc ccagaagaac aacgaggttc
     9541 gcgttgtctt caaggtcaaa gagcagcaac gggtgagcgt gcggcgcatc acgttcatcg
     9601 gcaacgatca catcccgagc gatgagctgc gtgcgctgat gtacaccggg aacgcgggct
     9661 tcttcgcgtt cggctccggc gggccattcc gccaggacgc cttcgaacgc gacatcgcgg
     9721 tgatcagcgc gagctactac gaccgcggct tcttgtcggt gtcgatcaac acgccgcgcg
     9781 tgatgctgac gccggacaag aacggcatcg aggtcgcgat cacgatcgac gaaggcccgc
     9841 gcttcaagat ccggcagcta cgtgtgttcg agcgtggcgc ggacgggcgc gaggtcgagc
     9901 cgatcgatgg gcggcgcaac ttgcgcatga tggtgcgggc cgagaccggt gactacttca
     9961 accgggcggc gctgctggaa gacctgcaga cgatccgcac gctgtaccgc gaccacggct
    10021 acgccaacgt ggacgcgacg ccggtcacga acgtcaaccc gcagaccaac gaggtggacg
    10081 taatcgtgcc cgtcgagcgc ggcccgctcg tccatttcga gcgcatcgag gtgcgcggca
    10141 acagcaagac ccgcgaccgt gtgatccggc gcgagatgga gatcgccgaa gggcaagagt
    10201 tcagcgagag cctgctcgag cgttcgcggc gacgcatcac cgcgctcggc tacttcgagc
    10261 gcgtggatgt ctctaccgag caaggctcgg cccccgacaa gatgaatgtc tacgtcgagg
    10321 tcacggagaa gcccaccggc accttccaaa tcggcgctgg tttctcgagt ctcgagaact
    10381 tcatcgccac ggcgcaggtg cagcaggcaa acctgttcgg caacgggcaa agcctctctt
    10441 tgcaggcgca ggtcagcggc atccggcagc tcgtcgacat tcgcctgtac gagccctact
    10501 tcttgaagag caagttcagc gccagcatca acctgttctc gcagctgcgc gtctatacgg
    10561 acttctccca aaccagcacc ggtggctcgc tcaccttcgg ctatccgctc ctcgagccgg
    10621 agctcacggc ctcgctgact tacacgggtc aagtcgatcg cgtctcgacg caggccgtgt
    10681 ccacgtggtt tggcagcggc acgggcaccg ccaccagcgt gttcgcgcgc ttgccgctcg
    10741 cgaacctgtt caacgacggt ttcacgtcga gcatccgccc cgggctcgtc tacgataccc
    10801 gcgacaaccg cttgtttccg acgagcggca tctacttgtc agcctcgacc gagttcgcga
    10861 ccagcgcgct cggctcggac aacttgttct tgcgccaccg tctgctcggt cgcttctact
    10921 atccgttggg cggcggcttc gtgctgaagt tgaactccga agcaggacac gtgacgagcc
    10981 cctcgagtga cggtgtgccg atcttcgcgc gcttcttcct cggcggcatc ttcgacgtgc
    11041 gcggcttccg ttaccgcacg ctcggcccgc gcgtgccact cactggcaac accgatccca
    11101 acagcttgcc aatcccgaac ggcgcgaaca tcggtggcaa cctgatgtac ttccagaacc
    11161 tcgagctcga gattccggtg ctcgagtccg tcggggtacg tggtgtgctc ttcaccgacg
    11221 cgggcaacgc ctggaacctc gagcagaact actgcggggc gaatccgggc gggcagatct
    11281 atcccgaaac cagcccgtgc ttcgatgggc tgaagagcct cgcacgtctg cgtacatcct
    11341 ggggcttcgg catccgctgg ttctccccgc tgggtccgct gcgtttcgag tggggttacc
    11401 cattcaagac cctgccctac gaggaaaagc acgtgttcga attcacgatc ggcaacttct
    11461 tctgagcctg catcgcgatc cgtccgcgcc gacggctccc cgaacactcg agccgctcac
    11521 gagcggactc gtcggacttg ccgccaaagt tgcagcggga gccgtcgccg ttcgctagca
    11581 ttgggggatg gccgccgctg atccgcgcga agctccgacg accgatcgaa ctccgggtct
    11641 cacgaccacg ggcgcggccg catccggacc ccgcgaggtg ggtcgagagg cgtcgcgagc
    11701 gagcctgagc cccaactcgg acacgctgct ttcggcgccc acgccggcta ctcgtgagga
    11761 ctttgcattc gacgaacggc tctccgaagc cgagcgcaga gtggcggaat tgctgctgcg
    11821 cgtcgagcac ttggagcgcg ccgccggcag accgagccaa aaccggcccc cgagagcgct
    11881 ttggttttgg ttcgtgttct tggcgggtct cgccttggcg tggcagatcc tgtcccgctt
    11941 tcggtagacg aacgacggac acgagcgcaa ctcgcgtccg aaactgaaca ttggacgcga
    12001 gctcgactcg cgcccgagat gaacatgggc tccagcctgg ctcggaagcc ctttccgata
    12061 gaggatagga agatgttacc gctcgtagga atcatcggtt ggcgcggaat ggtggggtcg
    12121 gttttggtcg agcgcatgct cgccgaaaaa gacttcgacg tgatcgaacc ccggttcttc
    12181 tcgacctcac aggcgggagg taaggcgccg aacatcgggc gcgacgtgcc ggccgtcggt
    12241 gatgccaagg atctcaaggc gctcgcgcaa aacgagatcc tgatcagttg ccagggcggt
    12301 gactacacga aggaaatcca cggcgcgttg cgggcgagcg gctggaaggg ctactggatc
    12361 gacgcggcga gcgcgctccg catgtccgac
//
LOCUS       DASTKU010000266         5465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1167248, whole genome shotgun sequence.
ACCESSION   DASTKU010000266 DASTKU010000000
VERSION     DASTKU010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167248"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VFQ61_09625"
     CDS             complement(<1..964)
                     /locus_tag="VFQ61_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9954755.1"
                     /translation="MLEPKPERCPKECAPTGNWIFGRSLHLAVYALALSGTVLGCTRA
                     EVNPKTPPPANPSAPDAMPPSSWQPSRSSHGALRDTPGELTVLDWAGFKGAVSYTFDD
                     ANSSQIEHFPELRALGVRMTFYLITSKPEARDATWQQALRDGHEIGNHTHTHQIQGTL
                     EDIDAAQDFLERTLGVRPTTMAAPYGAAMYSALAPHRFFINRGVRNGLIAPNDASDPF
                     NLNCYVPEPNAVAAALNEQVDAARAAGKWRIILVHGFAGGTDQAYQPIELREFVSSVE
                     HAKGLGDVWIDTVENIGAYWLGQRAFSRASRELHGGTTTWRWQLP"
     gene            complement(1168..1698)
                     /locus_tag="VFQ61_09630"
     CDS             complement(1168..1698)
                     /locus_tag="VFQ61_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954756.1"
                     /translation="MRTRACQRFPGAFAKRMGLFISTLALSLAGCRGYETAALDSKLC
                     PVGFNDEQHTSIVAAVQAWNDTGVSDLSVVEPGTPHCIPIYAQAGPTSMAASWGDIGH
                     SVSGMTAPDASRIDLLVDSAWTDESQELQSVAMHEICHLLSGPAHIQDQMDLMFTHQN
                     NVTVLSDYDIDRVINR"
     gene            2241..2474
                     /locus_tag="VFQ61_09635"
     CDS             2241..2474
                     /locus_tag="VFQ61_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954757.1"
                     /translation="MNKAAILCLGVALSMIVACGGSNKPAENPDGPAENAGEKADEAA
                     KDAGQAAEDASEKAGEKMEDAGDKVKEKTKDED"
     gene            2605..3054
                     /locus_tag="VFQ61_09640"
     CDS             2605..3054
                     /locus_tag="VFQ61_09640"
                     /inference="COORDINATES: protein motif:HMM:NF021082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2383 domain-containing protein"
                     /protein_id="HET9954758.1"
                     /translation="MILTDDLKKQTVDQLNSFLRGELSAAETYRQAIAKLPTSSARPV
                     LEDCARSHEERASMLVREVRRLGGDPAQDSGAWGSFVKLLEGGAQLFGEKAAIAILEE
                     GEDHGRNDYQRDLPDLEPKAKSVVVTQILPEQLRTHSAISSLKKNLS"
     gene            3155..4648
                     /locus_tag="VFQ61_09645"
     CDS             3155..4648
                     /locus_tag="VFQ61_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954759.1"
                     /translation="MTRMTGKGFHGASSRAWELPNAFRCTAAGVPLLLAWSVVACSVD
                     SSGRTYGSGGSSQGGSGASGSGGKTNDSGAGGEIGGESGSNQGGAESHGGSAGNAGAA
                     GETTQVTGGAGGSSDGAGGGGGEPPVCSAELLESSVTGSTVNGEDRFAPSCAPGRGND
                     LAFRFTAPATDYYRFTTRGSNFDTVLSLRDGTCDGAELACNNNDGASAQSKIVVQLEQ
                     GSESIVVVDGQLGAKGTVELAVEAVTCPSLDLTDQTLPAELTTQGASNEPHDGGCGGE
                     TLSEQAVRWRASEAGLYRFSASSSIFGPALYLEDGPECGGSLRQCNFNVVNGYPAQVT
                     RWLNEGEVVTLIVDGKQGMNGLFNLDVEKLPGACPSQPALAKAAANMVRLDDSTGKTL
                     LSSSCNWAGNRLDSRDRPFPEHSYPVHVALKTDQFCAYYLTSDSPVSVYVLEGASCDG
                     REVLCQAQVTEYEWHLDESKNGDYVLVVEDASSFHSGAVYSLTTDCM"
     gene            4733..>5465
                     /locus_tag="VFQ61_09650"
     CDS             4733..>5465
                     /locus_tag="VFQ61_09650"
                     /inference="COORDINATES: protein motif:HMM:NF021519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HET9954760.1"
                     /translation="MKWFKCECGASVYFDNTGCLACGRSLGFAPELRQMLAFTLPSSQ
                     PGSVAESQPSATEAQPSSGAAEVPSNPAPPNPGMPVVATDAEGKKHRQCANTAEGVCN
                     WLVREDDDCPLCVACRLNETIPDLSIAENRAKWARLEAAKRRLVFTLTRLQLEVVPKS
                     EAPDHGLAFDFKADTPALKVFTGHDNGLITLNIDEADPVRREQARLSLNERYRTLLGH
                     LRHESGHYYWDRLIRDDEPLLQEFRS"
BASE COUNT         1068 a         1635 c         1790 g          972 t
ORIGIN      
        1 ccggcagctg ccaccgccag gtggtcgttc caccatgaag ctcgcgagac gctcgactga
       61 aagcgcgttg cccgagccag tacgcaccga tattctccac cgtgtcgatc cagacgtccc
      121 ccaagccctt ggcgtgttcg accgagctca cgaactcacg taactctatg ggttgatacg
      181 cttggtccgt gccgcccgcg aagccgtgga ccaggatgat acgccacttg cctgcggcgc
      241 gcgccgcgtc cacttgctcg ttcaacgccg cggccaccgc gttcggctcc ggaacgtaac
      301 agttcaggtt gaacgggtcg ctcgcgtcat tgggtgcgat taggccgtta cgcaccccgc
      361 gattgatgaa gaagcgatgc ggcgcgagag ccgagtacat cgcggcgccg taaggagccg
      421 ccatggtggt cgggcgaacg cccagcgtac gctccaggaa atcttgcgct gcgtcgatgt
      481 cttcgagcgt gccctggatc tggtgcgtat gcgtgtggtt gccgatctcg tgcccgtcac
      541 gcagcgcctg ctgccaggtg gcatcgcgag cctcgggttt gctcgtgatc aagtagaagg
      601 tcatgcgcac gcccagcgcg cgcagctctg ggaagtgctc gatttgcgaa gaattcgcat
      661 cgtcgaaggt gtaggagacg gcgcccttga agcctgccca atcgagtacc gtgagctcac
      721 ccggcgtgtc gcgtaaggct ccgtgggagg agcgagaagg ttgccagctg ctcgggggca
      781 tcgcgtcagg cgcgctcggg ttcgctggcg gcggagtctt cggattgacc tcggctcgcg
      841 tgcatccgag aacggttcca ctcagagcga gcgcgtagac cgccagatga agcgaacggc
      901 cgaaaatcca gttgccggta ggggcgcatt cctttggaca ccgctcaggc ttcggttcga
      961 gcaccggcgc atactgtgcg cgttccagcc cccagttgca atactcgcga tgacacggct
     1021 agcagggcgc aggtacatca gccagggtct gcaccgcaga tcggagctgg caacgcgcgg
     1081 ccgcagcgca ggttccggcc tgggccacgg taaagacgga tagcagcgcg agaggcgcgc
     1141 cgcagcggca agcgagcggt cgcgggctca acggttgatg acgcgatcga tgtcgtaatc
     1201 acttagaacc gtgacgttgt tctggtgcgt gaacatcagg tccatctgat cttggatgtg
     1261 agcaggtccg ctcagcaggt gacagatctc gtgcatcgcc acactctgca gctcctggct
     1321 ctcgtccgtc cacgccgagt cgacgagcag gtcgatgcgc gacgcatccg gcgcggtcat
     1381 gcccgacacg ctatgcccga tgtcacccca agacgcggcc atactcgttg gtccagcctg
     1441 cgcatagatc gggatacagt gcggagtgcc cggctcgact acgctcaagt cactcacgcc
     1501 ggtatcgttc caggcctgca cggccgcgac gatcgaggtg tgctgctcat cgttgaatcc
     1561 caccgggcag agcttggaat cgagcgctgc ggtttcgtag cctcgacaac cggcgaggct
     1621 cagcgctagc gtgctgatga acagccccat acgttttgcg aacgcgcccg ggaagcgctg
     1681 acatgcccgt gtgcgcacct gcgatttcga atgccgcgcg aggtcgcggc cggtagacaa
     1741 acctcggcta ttgcgtccca tccgttccgt ggagccgatt cgggcccgcg cattggaaga
     1801 cgcgcagcgg ggtcagttat cgaatcggcg ttcctgcggg aaactacggt ggacttgcga
     1861 agctcttcag tcccaatgct cacgaccgtc aaagacttgt cgcacggggt cgcagcgtag
     1921 aggttgctcc tgcccagtcg tgagtccccc gagttgcatt cactcattct gcgagagctg
     1981 gagccatgcc gcggaacgac gcgggttctg gatgaagagc tcgccgcacc gaagttcggc
     2041 aagcaggcgt gaaaccggag ggtaggtcgg gggagaccga ggggcgagtt tgggcgaggt
     2101 ggggcgccag tgcgccccaa acctgccgca cgatccaact tgcctccgca ttatcaccaa
     2161 cttcatcgga gtgtgcgtct ggcacatagg ttgctcaacg gctgcagcgc gtcccccatg
     2221 gggacagagg agtcgttgaa gtgaacaaag cagccattct ttgcctcggt gttgcgttga
     2281 gcatgatcgt cgcgtgcggc ggcagtaaca agcctgcgga gaaccccgat ggccccgcag
     2341 agaacgcggg agagaaggca gacgaggccg cgaaggacgc cggccaagct gctgaagacg
     2401 cctcggagaa ggccggcgag aagatggaag acgccggtga caaggtgaag gagaagacca
     2461 aggacgaaga ctgagcttcg ggcccaaagg acgcagggag gccgaaggct ctgcgggtgc
     2521 agtgagggga gctctgccga tcaggcgcgt tcgcaacgtg aatgtgcgct cgttcggtgg
     2581 agctcggtcg agaggagacc agttatgatt ttgactgacg atctgaagaa acaaaccgtc
     2641 gatcaactca attccttcct gcgcggcgag ctttcggcag ccgagacata tcgtcaagcc
     2701 atcgcgaagc tgccgacgtc gagcgcgcgt cccgtgctcg aggattgcgc gcgctcgcac
     2761 gaagagcgcg ccagcatgtt ggtgcgtgag gtgcgacggc ttggcggcga cccggcgcag
     2821 gactcgggag catggggaag cttcgtgaag ctgctggaag gaggcgcgca acttttcggc
     2881 gaaaaggcgg ccatcgcgat cctcgaagaa ggcgaagacc acgggcgaaa cgactatcag
     2941 cgcgacctgc ccgacctcga gccgaaagca aagagcgtag tcgtaactca gatactgccg
     3001 gagcagctgc gaacgcacag cgcaatcagc agcctgaaga agaacctgtc gtaaccgcct
     3061 tcccacgctt ccggaatgac gcggaagcgc tcctaccctg tgcgactgtc cgttgcctct
     3121 cggcagctcc gcccgactcg gctagagtgc gaggatgacg cggatgacgg gcaaagggtt
     3181 ccacggcgcg agttccagag cttgggagtt gccaaacgct ttccgatgta cggcggctgg
     3241 cgtcccacta ttgctcgctt ggagtgtcgt cgcgtgctcc gtggatagct ccggacgcac
     3301 ctatggctcc ggcggttctt cgcagggggg ctcgggcgcg agtggttccg gtggcaagac
     3361 caacgatagc ggagctggcg gggagatcgg tggggaaagc gggtcgaacc agggcggggc
     3421 cgaatcgcac ggaggtagcg ctggcaacgc gggcgcggcc ggcgagacca ctcaagttac
     3481 gggtggagcg ggaggttctt cagacggggc cggcggcggc ggcggggagc caccggtttg
     3541 tagcgccgaa ctcctagaga gcagcgtcac tggcagcacc gtcaacggtg aagatcgctt
     3601 cgctccgagc tgcgcgccgg gtcgagggaa cgacctcgca ttccggttca ccgcgccggc
     3661 aactgactac taccgcttca cgactcgcgg ttccaacttc gataccgtgt tgtcgttacg
     3721 cgatggcacc tgtgacggcg ccgagctggc ctgtaacaac aacgacggtg ccagcgctca
     3781 gtccaaaatc gtggttcaac tggagcaagg ctcggaaagc atcgtcgtag tggatggcca
     3841 actcggagcc aaagggacgg tagagctggc ggtagaggcc gtaacttgcc ccagcctgga
     3901 tctgacggat cagacgctgc cggcagagtt gaccactcag ggcgcatcga acgaacccca
     3961 cgacggcggg tgtggcgggg aaaccctctc ggagcaggcc gttcgctggc gcgcctccga
     4021 agcaggtctt tatcgtttca gcgcgagctc gagcatcttt ggccccgcgc tctacctcga
     4081 ggacggtccc gagtgtggag gatcgctgag gcaatgcaac ttcaacgtcg tcaatggcta
     4141 tccggcccag gtgacgcgct ggctgaacga aggtgaggtc gtcacgctca tcgtagacgg
     4201 caaacaggga atgaatggtc tgttcaacct cgacgtcgag aagctgccgg gcgcctgccc
     4261 atctcaaccg gcgctcgcca aggcggccgc gaacatggtc cgtctcgacg attccacggg
     4321 caaaacgctg ctctcgagct cctgcaattg ggccggcaat cggctcgata gcagagatcg
     4381 gccttttccc gaacactcgt atcccgtcca cgtggcgctc aagaccgacc aattttgcgc
     4441 ctactatttg acgagtgatt cgccggtttc cgtttatgtc ctcgagggcg cgagctgcga
     4501 cgggcgagag gtgttgtgtc aggcgcaggt gaccgagtac gagtggcact tggacgagtc
     4561 gaaaaacggg gactacgtgc tggtcgtgga agacgccagc tcgttccatt ctggcgccgt
     4621 ctattcgctc acgaccgact gcatgtgatg cgtaatcgga tcagctcttg gacatctcgt
     4681 cgaaccagag ccaagcgtcc tggaacggcg cctgcattgc tcgcgcccaa tcatgaaatg
     4741 gttcaagtgc gagtgtggag cctccgttta tttcgacaac accgggtgtt tggcttgcgg
     4801 ccggagcttg ggctttgcgc ccgaactccg gcaaatgctc gcctttactc tgccgagtag
     4861 ccagcccggt agcgttgcgg agagccaacc gagcgccacg gaagcgcagc ccagttccgg
     4921 agccgcggaa gtgccttcaa acccagcacc tccaaacccc ggtatgcccg tggtcgccac
     4981 ggatgcagag ggcaaaaagc atcgtcaatg cgccaacacg gccgaagggg tgtgcaattg
     5041 gttggtacgc gaagacgatg actgcccgtt gtgcgtggcc tgccgtttga acgaaacaat
     5101 ccccgatctt tcgatcgcgg agaatcgagc caaatgggcg cggctggagg cggccaagcg
     5161 tcggctggta ttcacactca cccgcctgca gctcgaggtc gtgcccaaga gcgaggcgcc
     5221 ggatcatggt ttggcgttcg acttcaaagc ggacacgccc gcgctcaagg tgttcaccgg
     5281 gcacgacaac ggcctcatca ccttgaacat cgacgaagca gaccccgtgc ggcgtgagca
     5341 ggcgcgcctg tcgctgaacg agcgttaccg tactctgcta ggtcacctgc gacacgagag
     5401 tggccactac tattgggacc gtttgattcg cgatgacgag ccgcttctgc aagaatttcg
     5461 atcct
//
LOCUS       DASTKU010000267         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1614016, whole genome shotgun sequence.
ACCESSION   DASTKU010000267 DASTKU010000000
VERSION     DASTKU010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614016"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..897
                     /locus_tag="VFQ61_09655"
     CDS             247..897
                     /locus_tag="VFQ61_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954761.1"
                     /translation="MHQILRSQSFRYCSALALFGTLLLASGNVHAQSIIKRPGAHPRY
                     SVELEPHLALQWSNRFGGGDGIGPGVRVNIPFLDNGPIRSINNNMAIGVGLDLTFGDN
                     DCRWWWGRYDRNVWLGRENCSGTEVWAPLTLQWNFFLTPVISVFGEPGFAIAHRRYSW
                     EWWCNGANGNICGYHDSTTDVELVFWGGARFMFSDTVGATVRIGTPYVSAGINFLL"
     gene            complement(925..2511)
                     /locus_tag="VFQ61_09660"
     CDS             complement(925..2511)
                     /locus_tag="VFQ61_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0569 family flavin-dependent
                     oxidoreductase"
                     /protein_id="HET9954762.1"
                     /translation="MPEMHFRVRWPHGLIENCYSPSWIIEEYLAAGQSYTVQDFLERT
                     RTALNLASERVRARYGFACSSALDQLQALEATARELALRGKAGRVQVLALIRHAARDA
                     RSAEREPFSVIVVGAGQAGLSASYWLKSHGVPHVVLEGQRIAHAWRSERWDSFCLVTP
                     NWQCQLPGHGYAGPDPTGFMLKDEIVRYVESYAERYELPVREGVQVTSIRQSGCPSPR
                     FEVDTTRGRFSADHIVIAAGGYHTPRFPEFAGDLPDGILQLHSVSYKNPEALPEGEVL
                     VVGTGQSGAQIAEDLLLAGRQVHLCVGSAPRCARTYRGRDVVEWLDEMGHYRIPITQQ
                     KDPEALRDRANHYVSGRSGGHDLDLRAFARAGMKLYGPLIGVESGKLRFEPRLKQYLD
                     AADDVYRSINRAIDDYIAQRGIPAPPGASYQPVWEPGDECLELPLAETQITSAIFCTG
                     FDTDYSWIEADIFDERGKPKHQRGVTPVPGLYFVGLPWLHTWGSGRFEAVGRDAEFVV
                     QELLSEERARAPRSGQLLTG"
     gene            complement(2504..>3300)
                     /locus_tag="VFQ61_09665"
     CDS             complement(2504..>3300)
                     /locus_tag="VFQ61_09665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9954763.1"
                     /translation="LRECQARSIRQADLLLSASQATRLDVIRQFGLESTVVKNGVCAS
                     RFARPIDLEKLTARLPALGPTLPRFVLSVGGVEPRKNSIQALAAFAEVALVNAELHWL
                     IAGGASIWEHTAYRARFATELSKLPTSIAARVHVLGAVSETELEALYSSSSLLLHPAE
                     QEGFGLCVLEALASGCPVIVPRGAPFDEYLDDRTAAVVDPHSTASIASALQAVLNWSA
                     SERASRRAAGLITARLHDWQSVARTHLAAYESAMRLTPSCTEVLHA"
BASE COUNT          600 a         1038 c         1049 g          613 t
ORIGIN      
        1 ggtttcgcgg gactcacgcg cccgatcgca agcaggatgc gtaccacgcc ctgtggtgag
       61 ctgcgctaaa gcactcatgg ggcgagcgct cgattcttct gattggaaga tcatgatgga
      121 cggcgtttca cgtcgtaacc gcgggcccgc ccgcccggcc tgaccgtgaa cgctcgcgtc
      181 gtgccgttta gtttgcggac ccttccgaag ctgacgtaga aatcagcaac tcacggaggc
      241 agcctcttgc atcagattct tcggtcccaa agctttcgct actgctctgc cctcgctctg
      301 ttcggcacgc tgttgctggc atccggcaat gttcacgcgc agtcgatcat caagcgtccg
      361 ggcgcgcacc cgcgttacag cgtcgaattg gagccgcacc tggcgctgca atggtcgaat
      421 cgttttggtg gcggggacgg catcggcccc ggcgtgcgcg tcaacattcc gttcttggat
      481 aacggcccga tccgcagcat caacaacaat atggcgatcg gcgtagggct cgatctcacg
      541 ttcggagaca atgattgccg ttggtggtgg gggcgatacg accgcaacgt ttggttgggc
      601 cgggagaatt gtagcggaac cgaggtctgg gcgccgctaa cgctacagtg gaactttttc
      661 ctgaccccgg tgatcagcgt gttcggagaa ccgggcttcg ccatcgctca ccgacgttac
      721 agctgggagt ggtggtgcaa cggcgcgaac ggcaacatct gcggctatca cgactcgacc
      781 actgacgtgg agctggtgtt ctggggtggc gcacgcttca tgttcagcga cacggtggga
      841 gcgacggtgc gaatcggcac gccctacgtc tcggcgggta tcaacttctt gctctgagcg
      901 cacttcccct gcagccttcg atgctcaccc cgtgagcagt tgccccgagc ggggagcgcg
      961 agcgcgttcc tcgctcagca actcttgcac gacgaattct gcgtcgcgac caacagcctc
     1021 gaagcggccc gacccccagg tgtgcagcca cggcaagccg acgaagtaga ggccgggaac
     1081 cggagtcact ccacgttggt gcttcggctt ccctcgttca tcgaatatgt cggcctcaat
     1141 ccagctgtag tccgtgtcga agccagtaca gaagatcgcg ctagtgatct gggtctccgc
     1201 caacgggagc tcgagacact cgtccccggg ttcccaaacg ggctggtagc tcgcgcccgg
     1261 cggggctggg atgcctcgct gggcaatgta atcgtcgatc gcgcgattga tgctgcgata
     1321 gacgtcatcg gccgcgtcga ggtactgctt gaggcgcggt tcgaaccgca acttgccgga
     1381 ttcgaccccg atcagcggcc catagagctt cattccggct cgcgcgaagg cccgaaggtc
     1441 caagtcatga ccaccgctgc gtccagagac gtagtggttg gctcggtcgc gcagcgcctc
     1501 tggatctttc tgttgagtga tcgggatgcg gtagtggccc atctcatcga gccactccac
     1561 cacgtcgcgc ccccggtacg tgcgcgcgca gcgcggagcg ctcccgacgc acaggtgaac
     1621 ttggcgccct gcaagcaaca gatcttccgc gatctgcgct ccggactgcc ccgtcccgac
     1681 gacgagcacc tccccttccg gcagagcttc tgggttcttg tagctgaccg agtgtagctg
     1741 caggatgccg tccggcagat ccccggcgaa ctcggggaag cgtggagtgt gataaccgcc
     1801 cgcggcgatc acgatgtgat cggcggagaa gcggccgcgg gtcgtgtcga cctcgaagcg
     1861 cgggctaggg cagccactct gacgaatcga ggtgacctgc acgccctcac gaaccggaag
     1921 ctcgtatcgc tccgcgtaac tctcgacgta gcgaacgatt tcgtctttga gcatgaaacc
     1981 ggttgggtct ggcccggcat agccgtggcc cggcagttgg cattgccaat tgggagttac
     2041 gaggcaaaac gagtcccatc gctcgctccg ccatgcatgg gcgatgcgtt ggccttcgag
     2101 taccacatgc ggcacgccat gggacttcag ccagtagcta gccgacagac cggcttggcc
     2161 agcgccgacc acgataacgc tgaagggctc gcgttcagcg ctccgggcgt cgcgcgctgc
     2221 gtgccggatc aaggcaagga cttggacccg accagccttt ccgcggaggg cgagctcgcg
     2281 cgcagtcgct tcgagcgctt gcagttggtc gagcgccgag ctgcaggcaa agccgtagcg
     2341 ggctctgacc cgttcagacg caagattcaa tgcggtgcgg gtgcgttcca gaaaatcctg
     2401 gacggtgtag ctctgcccgg cggccaagta ctcttcgatg atccaagacg gcgagtagca
     2461 attctcgatg agcccatgtg gccaacgaac gcggaaatgc atttcaggca tgaaggacct
     2521 ctgtgcacga cggcgtgagc cgcatggctg attcataggc ggcgaggtgg gttcgcgcga
     2581 cgctctgcca atcgtggagg cgcgccgtga ttagccccgc cgcacggcga cttgctcgct
     2641 cggatgcact ccaattcaga accgcttgga gtgcggaagc aatgctggcg gtcgagtgcg
     2701 gatcgacaac ggctgccgtc ctgtcgtcca agtactcgtc gaagggcgca ccgcgaggaa
     2761 cgatcacggg gcagcccgac gcgagcgctt ccagcacaca caacccaaat ccctcctgct
     2821 cggcgggatg tagcagcaac gatgatgagg agtagagcgc ctccagctcg gtctcgctca
     2881 ccgcgcccag cacgtgcacg cgggccgcaa tggaggtggg cagtttggag agctccgtgg
     2941 cgaagcgcgc ccgataagcc gtgtgctccc agatgctggc ccctcccgcg atgagccaat
     3001 gaagctcggc gttcaccagc gcaacctccg cgaaggcggc cagcgcttgg atgctgtttt
     3061 ttcgcggttc caccccaccg acgctcagca cgaagcgcgg gagggtcgga ccgagcgcag
     3121 gtaggcgggc cgtgagcttt tccaggtcta ttggtctcgc gaagcgcgag gcacacaccc
     3181 cgttcttcac gaccgttgac tccagcccaa actgccgaat cacgtccaag cgtgtggcct
     3241 ggctcgcgct caacagcagg tcggcttgcc gaatggatcg cgcttggcac tcacgcagat
//
LOCUS       DASTKU010000268         8679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_274047, whole genome shotgun sequence.
ACCESSION   DASTKU010000268 DASTKU010000000
VERSION     DASTKU010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274047"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="VFQ61_09670"
     CDS             <1..1342
                     /locus_tag="VFQ61_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9954764.1"
                     /translation="AAASSPLRLGMSGPLQGPSQDLGIEMRRGIMARLDAENQRGGLN
                     GRKLEFSCLNDNYDPTLAVENTRQLLNITQLEDGADQPDQRGPNGVLALLGSIGTPTM
                     LATAPIATKNRVVFFAPFTGSQKYLRDGTNSPYVYNFRAGYYDETNAMVDYMANYRSP
                     RIITGPDSYKHLIAFTQKDAYGDAGYDGLVTAYNTRVAALPQPDSTQPNPSIARLYYE
                     RESVSSVDPAVEQATALLADILGDGQTRQSVGIVMIDTYQPGNKFIRGVKDWLNQDAT
                     RAAQLDVLFMHVSFVGSDSLSKALTSPPESYVDVTDPAARRKKTYAADVMVTQVVPYY
                     KSQASGVAAYRSDLAAFDGGTTSFTSLEGYLAASVLIEGLKGIRGDISSEALVHALDS
                     NMSDIDVGIGAPLSFSSTNHQASRTVWGSVLTDDGTFKVPFMWNPKDKIIPGSE"
     gene            1584..2981
                     /gene="sthA"
                     /locus_tag="VFQ61_09675"
     CDS             1584..2981
                     /gene="sthA"
                     /locus_tag="VFQ61_09675"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HET9954765.1"
                     /translation="MLNDFDLVVLGGGPAGQKAALQGAKSGCRVLLVDRARTVGGSCL
                     RFGAIPSKALRETAVALHRLERLTGRASAPSGIDLGALLDRQEHIIHSHERYSHQQLS
                     RAGVTIWQGVAHFRSPHEVEVRQSEGGRDRVRARWFVIATGSYPMNNGSIPIDHEHVL
                     DSDSILSLTSLPESLLIIGSGVVAAEHASTFAALGTRVTLVDSAHRPLAFLDEELTSV
                     FVSEFSRLRGRYLGERELRHLRIAADGRVHVELDRGEKLKFDKVLSAVGRKAAISGLG
                     LEAAGVRVNDREFITVDENCQTNVKHIYAVGDVAGPPAMASTAMAQGRRAICHALGIQ
                     LREGRDVAPLGIYTIPEIASVGMTEDEATAKYGEIVVGRAPFHELTRGKLASAESGLL
                     KLVCDAQSGRILGVHAVGENATDLVHVGQVAMLGGVRYDAFIDTVFNFPSYAEAYRVA
                     TLDILSRERTRNAAE"
     gene            complement(3046..7311)
                     /locus_tag="VFQ61_09680"
     CDS             complement(3046..7311)
                     /locus_tag="VFQ61_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9954766.1"
                     /translation="MTTELTVLGRQCVLSERLGRGAAGDVWVARSATGEPSETFEWVL
                     KLAHDEGGYEWIASEAERLLCVDSPHCVRLLHAFTLSENVEAHAGGEETRFAAGTPCL
                     VFERSAGVALDPVRGGADRVQRLLQVARDVARGLADLHATGMAHGDIKPANILVSESQ
                     VDIAGNSRDSDLFATLVDFGLSGSLREETARGGTRRYLAPECFSGERAGDARFRDLYA
                     LGVTLAELASPEVAADASPERAFLGQHLPHEVKRLVAALLSGVPAARPSAEWVWGRAL
                     ALLGETESPEACHARRVSAIRRTYLTVRRAQLSLAASAAGSQIESEGVVGQWLRDALG
                     LFRSVAALRRRDAISKPIALADLDAVGRARWLVELCGVTAAAWPELPVSSDAELAQRL
                     LDLASEHDPSRVTFAELDAGARAAPSIASDWASSSSGVVRIALAMGRGPCSRAMLDAA
                     EQLLARDGDALPEAHAELGLALGRSLRLRGELGRALCVLDRVATPAARLEIAEVRRRA
                     GDALGASACIRELCSIDLPGDLAAKAQAICARLQLDTGDAAGALALLDENTGAAAASV
                     ASLEVRALALLGLGQKAAAHEAATRARLLAEGDEERARVEAVFGNLHHFEGQFDLALQ
                     AFRAASEHAARAGAALEEASYLTGVSAAAAQVGELGLAGSCARRALLLFQGLRRPREA
                     ARAQLSLASTLASVGARLEAGEQARECADRAEASGDVRCQAYAHLVWCDVAEDPSKAA
                     ECAHTARGLLEPTLGQGDGDDTLRVSARLHEYARVTDVAGIDSAAERPSAALDAVLEW
                     WAARARVAEARTSRELGADTARILRALLGAVGRAAPSTVRGPAFAAGAALAARLKDAD
                     AARRLSAAAADAARELLRRAGPDFEAQVRALPWVRMADLVQTSPFSPEQLTDVERLVR
                     ALGQRDRLRPLLEQVVDALVLWTGVERGLLLLRAPGGKLRPRAARNLFKADLVGPQLE
                     LSHSLSERALALGEPVVAVDATGDLPLVHESVHALKLRSVLAVPLIARGQALGVVYLD
                     DRMRRGAFGARELSWVRLVATLAALAIADARDQILLRRAARRATRAESRLREELSRRE
                     AELDVAVRELSRSRGARDTRFPYADIVGDSESVRRMLRLVDRVACSDVPVLIQGESGS
                     GKELVARAIHRHGQRQERPFVVENCGAIPEPLLESALFGHVRGAFTGADRARAGLFEV
                     AHGGTLLLDEIGEMSLGMQAKLLRVLQDGEVKAVGSDKARRVDVRVIGATHRDLEALV
                     AAGKFREDLYYRLNVISIRVPPLRERVGDIPILVKHFVARHAGERRVQVSRPALERFC
                     AYPWPGNVRQLENEIRRVLVLADEVVGEEHLSPELVQKTQGKAIDELNLRQRIDALEA
                     ELVRVALRRTEGNQTRAAELLGVSRFGLQKMMKRLEISSVSRA"
     gene            7664..>8679
                     /locus_tag="VFQ61_09685"
     CDS             7664..>8679
                     /locus_tag="VFQ61_09685"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9954767.1"
                     /translation="MSDDAAVLIIDDDDLVRRAFRRTLERGGYAVREAKNGRDGLAQA
                     ARQLPAAVLLDLRMPGLDGLDVLSGLVQDHPDLPVVVVSGQGTMEDVVEALRRGAWDF
                     LSKPVMDPEIFVRTIARGIEKATLIRQNREYSETLRGTNQRLSAALDELRNDEQAARA
                     LQFQLLPEDDLELCGYRFSRRLLPSHLLSGDFLDYFPLGERQVGFYLADVSGHGAASA
                     FVTAILTTLVSKYREGLVSREDATLLEPAQLLARLDADLAPLGLPKHVTMFYGVLDAD
                     RNQLRAASAGAFPFPLVRRGASGAGSELECSGRPLNLPGPRQFGTVETSLPSGSALLL
                     ASDGV"
BASE COUNT         1648 a         2974 c         2634 g         1423 t
ORIGIN      
        1 ggcggcggcc tcgagccccc ttcgccttgg catgagcggc ccattgcagg gcccgagcca
       61 agacctcggc atcgagatgc gacgcggcat catggcgcgc ttggacgcag agaatcagcg
      121 cggcgggctg aacggacgca agctcgagtt ctcgtgcctg aacgataact acgacccgac
      181 gctcgcggtc gaaaatacca ggcaactcct gaacatcacc caactggaag acggcgcgga
      241 tcagccggac caacgcggcc cgaacggcgt gctcgcgttg ttgggcagca tcggcacgcc
      301 caccatgttg gcaaccgcgc cgatcgcgac caagaatcgg gtggtgttct ttgcgccctt
      361 cacgggctca caaaaatacc tgcgggatgg caccaattcc ccgtacgtct acaacttccg
      421 cgcgggttac tacgacgaga ccaacgcgat ggtcgactac atggcgaact atcgctcgcc
      481 gcgcatcatc accggaccgg actcgtacaa gcacctgatc gcgttcacgc aaaaggacgc
      541 ctacggtgac gcgggctacg atggtctcgt caccgcttac aacacgcgcg tcgcggcgct
      601 accccagccg gactccaccc agcccaaccc ctcgatcgcc cggctctact acgagcgtga
      661 gagcgtgagc agcgtcgacc ccgccgtcga gcaagcaacc gcgctcttgg cggacattct
      721 gggtgacggc cagacgcgcc aaagcgtcgg catcgtgatg atcgatacct atcaaccagg
      781 gaacaagttc attcgcggcg tcaaagattg gctgaatcag gatgcgaccc gagcggcgca
      841 actcgacgtg ctgttcatgc acgtttcgtt cgtcgggagt gactctctct ccaaggcgct
      901 aaccagccct cccgagagct acgtggacgt caccgacccc gccgcgagac gcaaaaagac
      961 ctacgccgcg gacgtgatgg tcacgcaggt ggtcccctat tacaagtccc aggcttcggg
     1021 ggtagcagct tatcgcagcg acttggccgc cttcgatggg ggcaccacca gctttacttc
     1081 gctggagggt tacttggccg ccagcgtcct gatcgagggg ctcaagggca ttcgcggcga
     1141 tatctccagc gaggctctgg ttcacgcact cgatagcaac atgagcgaca tcgacgtggg
     1201 catcggtgcg ccactcagct tctcctccac caatcaccag gcgtcgcgca cggtgtgggg
     1261 cagcgttctg acggacgacg gcaccttcaa agtgcctttc atgtggaatc cgaaggacaa
     1321 gatcatccct ggatccgagt agttcgacgc acgaatcctg gatcggagtt cgcgtctcga
     1381 tctgaactcc gttaaccatt ccgaaacaac actactgcac acgagtggcg gctgacggtc
     1441 cacgcaccgg ctgtcggaaa acaccgacgg agaagcagtc gaaaagcgtc attcggattg
     1501 gacactccaa ttcgaattca tcgtcacgat ttctcgcctt ggacgggccc gacgcggctc
     1561 ggcctgacat gctttcgggt cccatgctga acgatttcga tctcgtggtg ctgggcggcg
     1621 gccctgcagg gcaaaaggcc gcgctgcagg gcgccaaatc tggctgtcgc gtgttgctcg
     1681 tagatcgcgc gcgtacggtt ggtggttcgt gcctgcgctt cggcgccata ccgagcaagg
     1741 cgctccgcga gaccgcggtg gcgctgcatc ggctcgagcg gctgacaggg cgcgcctccg
     1801 cgccctccgg catcgattta ggcgcattgc tcgatcggca agagcacatc attcacagtc
     1861 acgagcgcta tagccaccaa caactgagcc gcgccggcgt aacgatttgg cagggcgtcg
     1921 cgcacttccg ttctccgcac gaagtggagg tgcggcagag cgagggtggg cgggatcgag
     1981 tacgagcgcg ttggttcgtg atcgccaccg gctcataccc catgaacaat ggcagcatcc
     2041 ctatcgatca cgagcacgtg ctcgatagcg attccatcct ttcgctgacg agcctgcccg
     2101 agtctctgct catcatcggg tctggcgtgg tggctgcaga gcatgcctcc accttcgcag
     2161 ccctcggcac tcgcgtcacg ctcgtagatt ccgcgcatcg cccactcgcg ttcttggacg
     2221 aagaactcac cagcgtgttc gtctccgagt tttcacgact gcgcggacga tacttggggg
     2281 aacgcgagct acgtcacctc aggatcgcag ccgacggccg cgtgcacgtc gagctcgatc
     2341 gcggcgagaa gctgaagttc gacaaagtgc tctcggcggt gggacgcaag gccgccatca
     2401 gcgggctggg gctcgaggcc gccggcgtcc gggtcaacga tcgcgagttc atcacggtgg
     2461 acgagaattg ccagacgaac gtcaaacaca tctatgccgt gggcgacgtc gccggaccgc
     2521 ccgccatggc ctccaccgcg atggctcagg ggcgtcgcgc catttgtcac gcgctcggga
     2581 tccagctgcg tgaagggcgc gacgtggccc cgctcgggat ctacaccatc cccgagatcg
     2641 cgagcgtggg catgaccgaa gacgaggcga ccgccaagta cggcgagatc gtggtcgggc
     2701 gggccccatt ccacgagctc actcgcggca agctagccag cgccgagagc ggcttgttga
     2761 agctggtctg cgacgcgcag agcggccgca tcctcggcgt gcacgcggtc ggggagaacg
     2821 cgaccgatct ggtccacgtg ggacaggtcg cgatgctcgg cggcgtacgc tacgacgcct
     2881 tcatcgacac cgtcttcaac ttcccgagct acgccgaagc gtatcgcgtg gccacgttgg
     2941 acatcttgag ccgcgagcgg acccgcaacg cggccgagtg agcccacccc tcgagtgagc
     3001 tcaccagccg cgcgcccagc caagaccgcg gctgaattcg aaagctcacg ctctcgaaac
     3061 gctcgagatc tcgagtcgct tcatcatctt ctgtaacccg aagcgagaca caccgagcag
     3121 ctccgcagcc cgggtttgat tgccttccgt gcggcgcagg gccacccgca ccagctccgc
     3181 ttcgagggca tcgatgcgct gtctgaggtt caactcgtcg atggcctttc cctgtgtctt
     3241 ttgcacgagc tcggggctca ggtgctcctc accgacgacc tcgtccgcca acaccagtac
     3301 gcgcctgatc tcgttctcga gttgccgcac gttacctggc cagggatacg cgcagaatcg
     3361 ctccaatgcg ggccgtgaaa cttgtacgcg gcgctcgccc gcatgtcgag cgacgaagtg
     3421 cttcactagg ataggaatgt ctcccacccg ctcacgcaag ggcggcactc ggatcgagat
     3481 cacgttcagc cgatagtaaa ggtcctcgcg aaacttgccg gccgcgacca aagcttcgag
     3541 gtctcggtgc gttgctccaa tcacgcgcac gtccacgcgt cgtgccttgt ccgatccgac
     3601 cgctttcacc tcgccgtctt gcagcacgcg cagcagcttg gcttgcatcc ccaggctcat
     3661 ctctccgatt tcgtcgagca acagggtccc gccatgagcg acctcgaaga gccccgcacg
     3721 cgcgcgatcg gcgcccgtga acgcaccccg cacgtgaccg aacaacgccg attccagcaa
     3781 cggctccgga atggctccac aattttccac gacgaagggg cgctcttggc gttgaccatg
     3841 ccggtgaatg gcgcgggcca ccagctcttt tccggagcct gattcgcctt gaatcagaac
     3901 gggcacatcc gagcaagcaa ctcgatcgac gaggcgcagc atgcgcctca cgctctcgct
     3961 gtcccccacg atatctgcgt acgggaagcg tgtatcgcga gcaccgcgcg agcgagaaag
     4021 ctcacgcacg gcgacatcca gttcggcctc acgtcgagat aactcctctc gcaagcgcga
     4081 ctccgcgcgt gtagcccggc gcgccgcacg gcgcagcaga atctgatctc gagcgtccgc
     4141 gatcgcaagc gccgccaagg tggcgacgag acgcacccaa gacagttcac gcgcgccgaa
     4201 ggccccccga cgcatgcgat cgtccaggta gaccactccc aaagcttgcc cgcgtgcaat
     4261 cagaggcacg gccagaacgc tgcgtaactt caacgcgtgc acgctctcgt gcaccagcgg
     4321 gagatcgccc gtggcgtcta ccgcgacgac cggttccccg agcgccagcg cccgctccga
     4381 gagtgagtgg ctgagctcga gctgtgggcc cacgagatcg gccttgaaca ggttccgcgc
     4441 cgcgcggggg cgcaacttgc cgccgggagc gcgcaataac aagaggccgc gctctacccc
     4501 ggtccaaagc acgagcgcgt ccaccacttg ctcgagcaac ggacgcaatc gatcgcgctg
     4561 ccccagtgct cgcaccaagc gctcgacgtc ggtgagctgc tccggcgaga aggggctggt
     4621 ttgcaccaag tccgccattc tcacccaggg tagtgcgcgc acctgcgctt cgaagtcggg
     4681 gcccgcccgg cgcaggagct cccgggccgc gtcggcggcc gctgcgctca agcggcgcgc
     4741 cgcgtctgcg tctttgagac gcgctgccag cgctgctcca gccgcgaatg cgggaccacg
     4801 aacggtgctc ggcgcagctc gcccgactgc cccgagcagt gctctcaata tccgcgccgt
     4861 atccgcgccc aattcccgag acgtacgtgc ctccgcgacg cgagcgcgcg ccgcccacca
     4921 ttcgaggacg gcgtcgagcg ccgcgctcgg gcgctcggcg gccgaatcaa tgcccgcgac
     4981 gtccgtgacg cgcgcgtatt cgtggagccg cgccgagaca cgcaaagtat cgtcgccatc
     5041 accctgaccc aacgttggct cgagtaagcc acgcgccgtg tgcgcgcact cggccgcctt
     5101 agaagggtct tctgcgacgt cacaccagac tagatgcgcg taagcttggc accgcacgtc
     5161 cccgctcgcc tccgcgcgat ccgcgcactc gcgagcctgt tcacccgcct ccaaccgtgc
     5221 accgaccgaa gccaaagtgc tggcgagcga gagctgtgcc cgcgccgcct cccgaggccg
     5281 ccgtaggcct tggaacaaca acagcgctcg ccgcgcgcag gaacccgcga gcccgagctc
     5341 gcccacctga gctgcggccg cagaaactcc agtcaaatag ctggcttctt cgagggcagc
     5401 cccggctcgg gcggcgtgct ccgacgcagc acggaatgcc tgtaaggcca gatcgaactg
     5461 gccctcgaaa tggtgcaagt tgccgaacac ggcctctacg cgggcgcgct cctcgtctcc
     5521 ctcggccaac aagcgcgctc gagtcgcagc ttcatgtgcc gcggccttct gcccgaggcc
     5581 aagcagcgcg agcgcccgaa cctccaagct cgctacgctc gctgcggccg cgcccgtgtt
     5641 ctcgtccaag agcgccaatg cgcccgccgc gtcgcccgta tccagctgca atcgcgcgca
     5701 gatggcttgc gccttcgcag ctagatcgcc gggcaggtcg atcgaacaga gttcccgaat
     5761 gcacgccgac gcgccaagag cgtcgcccgc tcgtcgacgc acctcagcaa tctcgagccg
     5821 agccgccggg gtcgccacgc gatcgagcac gcacagggcg cgcccgagct ccccacgcaa
     5881 gcgtaaactt cttccaaggg ccaggcccaa ctcggcgtgc gcttctggca atgcgtcccc
     5941 atcgcgtgcc agcagctgct ccgctgcgtc gagcattgcg cgcgaacagg gcccccgtcc
     6001 catggcaagc gcgattcgga cgactccgga agaagaagaa gcccagtcgg aggcaatcga
     6061 aggggccgcg cgcgcgcccg cgtccagctc cgcgaacgta acccgcgagg ggtcgtgttc
     6121 ggacgccaaa tccagcagtc gttgcgcgag ctcggcgtcg gagctcaccg gcagctcggg
     6181 ccaggccgcg gccgtcacac cgcacagctc gaccagccaa cgcgcgcgcc ccaccgcgtc
     6241 cagatcggcg agcgcgatcg gtttggagat cgcgtcacga cggcgtagag ccgccacaga
     6301 tcggaacagc cccagcgcgt cccgtagcca ttgcccgacc acgccttcgc tctcgatctg
     6361 cgaccccgca gcactcgccg ccagagaaag ctgcgcgcgc cgcaccgtca aataggtgcg
     6421 gcggatcgcg ctcacgcgcc gcgcatgaca cgcctcgggc gactcggtct cacccaacag
     6481 cgccagcgcc cgaccccata cccactcggc gctcgggcga gccgctggca caccgctgag
     6541 cagcgccgcc accagtcgtt tcacctcatg gggtaagtgt tgtcccagaa acgctcgttc
     6601 aggtgaggca tcggcagcaa cctccggcga agcgagttcg gccagcgtca ctcccagggc
     6661 gtataggtct cgaaatcgag catccccggc gcgttcaccc gaaaagcact ctggcgcgag
     6721 atagcggcgc gtgccccctc gagcggtctc ctctcgtaga ctccccgata ggccaaaatc
     6781 cacgagtgtg gcaaacaagt cagagtcgcg tgagttgccg gcgatatcaa cttggctctc
     6841 gctcaccaga atgttcgcag gcttgatatc gccatgagcc attccggtcg cgtgcagatc
     6901 ggcgagcccc cgggctacgt cacgggccac ctgtaaaaga cgctgcaccc gatcggcacc
     6961 gccccgcacc gggtcgagcg caactcccgc gctgcgctcg aataccaagc agggagttcc
     7021 agcagcaaac cgcgtctcct cccccccagc gtgggcttcg acgttctccg acaaagtgaa
     7081 ggcatgcagc aaacgcacgc aatgcggaga atccacgcac aacaagcgct ctgcttcgct
     7141 ggcaatccac tcgtatccgc cctcgtcgtg agccagcttc agcacccact cgaacgtctc
     7201 ggacggctcg cccgtcgcgc tgcgggcgac ccaaacatca ccggcggctc ctcggccgag
     7261 ccgctcgctc aacacgcatt ggcggccgag caccgtcaac tcagttgtca tatcgaggaa
     7321 cccccagaca ttcgggaatt cagccacaaa caagtcgcat agtcaaccaa gatgccagag
     7381 gactcagctc acttcaggat tcccaaaacg gccggttcgg aacccgacct ttgcctagac
     7441 gttcgcccgg agcttgccca gtagaccgaa accaactcgt ccacgcgagg tcgggtcatc
     7501 agcccgggac gttcgaatcg cgcagcaatc gagatgagcg aaaggacgag cactcgaaaa
     7561 ggagctcgcg acatgacgtg ggagcggcga ttgcatgtcg aactccgcga gacgaaatgc
     7621 ggcttacgtg gtgtaggcga tctgttatgt tgaaaaacgg tccatgagcg acgatgcagc
     7681 agtgctgatc atcgacgacg acgacctggt caggcgcgcg tttaggcgta ccctcgagcg
     7741 cgggggctac gccgtgcgag aagccaagaa cggccgtgat gggctagcgc aagcggctcg
     7801 tcaattgcca gccgcggttc tgctggatct gcgcatgcca gggctggacg gcctggacgt
     7861 actgagcggc ctggtgcaag accaccccga cctgcccgtc gtggtggtgt ccggacaagg
     7921 caccatggaa gacgtcgtcg aggcgctgcg acgaggtgct tgggatttct tgtccaagcc
     7981 cgtgatggac ccggagatct tcgtacgcac gattgctcgc ggtatcgaaa aagcgactct
     8041 gatccgtcaa aaccgtgaat acagcgagac actgcgcggg accaaccaac ggctctccgc
     8101 ggcgctggac gaactacgga acgacgagca agccgcccgc gcgttgcagt ttcaactgct
     8161 acccgaggat gatctggaac tgtgcggata ccgcttttca cgccgactcc tgccctcaca
     8221 cctgctgagc ggtgacttct tggactattt cccgttggga gagcggcagg tgggcttcta
     8281 tttagccgac gtctcgggac atggcgcggc ttctgcattc gtaactgcca ttctgactac
     8341 gctggtttcc aaataccgag aggggttggt ttcgcgagaa gatgcgacac tgctcgaacc
     8401 tgcgcagctg ttggcgcggt tagacgcgga tttggctccc ctcggcttac ccaagcacgt
     8461 gactatgttc tacggcgtgt tggacgccga tcggaaccag ctgcgcgccg cgagcgcggg
     8521 agcgttcccg tttccattgg tgcgacgcgg ggcctcgggt gcgggaagcg aactggagtg
     8581 cagtggtcgt cccttgaact tgccagggcc acgccagttc gggacggtag agacgagcct
     8641 gccgagcggc agcgcgctac tcttggcgtc ggacggcgt
//
LOCUS       DASTKU010000269        13805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_944103, whole genome shotgun sequence.
ACCESSION   DASTKU010000269 DASTKU010000000
VERSION     DASTKU010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13805
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944103"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFQ61_09690"
     CDS             <1..477
                     /locus_tag="VFQ61_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954768.1"
                     /translation="ASGPLLAGGCGSRHSLPVVRRLPVVQTAASEDAEAMARPAAHWV
                     AIGVALMVALFLPLSMLGVAVAQAGFKAPAAGALFALSASSVGSGAVLGRFGLKLKAP
                     GAALAGALGGAALLLLSRLGGNRVSGGLFLFEAGLLIGLGALFCFAGFRLGHRRRP"
     gene            complement(379..2829)
                     /locus_tag="VFQ61_09695"
     CDS             complement(379..2829)
                     /locus_tag="VFQ61_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954769.1"
                     /translation="MCCVTALFITHASPSFAQTNLLAGKSAIRSQGMKNARALTDEAK
                     APEGDEWNTSAAAIIGPEQGFIEFDLGQSETIKAAYLQGDNNDEYLLTVSEDRKTYAP
                     LWTAPTTPEAGLRERFTDQLNARGRYVRLTARGGDRSYSVTELQLFSSRPAKFPPGLR
                     IATGQAQNSLVRTQFMYLGFAFFVLLFGTFARSKAWVIGLLGLGVAVAAYLAYQSVEF
                     GWPLASREVAFARATAAAIALFAVCRVALPAKRWPAHRRIVAGSLMFSAALALAAFYN
                     LGRPQFWNHQEGRPTFVHTYDMRVYQPFAKYFHELRYDGVYPASVLTYAEDVYGGSLE
                     PMARVEVRSLKDHKARRVADVKDEMLAIKQRFTPERWEALRQDMKYFREVMGPEFIGT
                     LTDHGANATPVWVFFARLFLGHTSASDGVLTIAGLADAALFLLLGIAMWRTFGLLPAL
                     AALTVLGALDLYMFGTNWTGATLRHDWMAFLGFAACSLKAHRFRLAGVFMALAALIRA
                     FPVVALFGVALPAIWWVAETWRKEGKPPTIKRILERHKDAVRVLLSAAACVAVAIAVT
                     SALYSFESWLQWWQKVTLLNRDVGVNEVSLRALVAGVDSSSARAGQARFLILGSAKLL
                     CVVLVAIACRKRPLYQAMLLSLPLVQVMWNPSNYYSHFIFLLVLLGSGATKAQAALAH
                     EDEAARVEGLPLRLLQSAGPLLGLCIAGYWISLEPDLDRHFQDTTVTLFVALGWLMAN
                     VWRTDPAVRAFLKGEPTKSEPSEERGPRSEPPLREDADGDEAPSSRPAPVPQTKTREA
                     EQRPQPDQQAGLEQEEPA"
     gene            complement(3168..3815)
                     /locus_tag="VFQ61_09700"
     CDS             complement(3168..3815)
                     /locus_tag="VFQ61_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954770.1"
                     /translation="MAMPKATPATLGDDSPDSSGSISRRTEPFGAEPSRSEPNRSEPS
                     QTEQSSSEQSSSELSGVRPSVLPPGPLELPEDVASRFDAALAAHARTEAALTDLSRTV
                     KFLSASISSIRDAHQRLGSELGSLLGRLQNDDPSRALKRRIQHLEHVVVETSRSAARE
                     RDFLVADHDAFIFTLISEHEREIAALRERMTEFEPHSATRLTSTTGDLPDGPERG"
     gene            complement(4008..4268)
                     /locus_tag="VFQ61_09705"
     CDS             complement(4008..4268)
                     /locus_tag="VFQ61_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954771.1"
                     /translation="MDLAEWRSEVARLLEESAAALRNKSDRKCAVAFGTALGYLEKAR
                     ARGDVMANTLLAQFVLTEEDSTQRFTFAPGELEALNPKKTER"
     gene            complement(4331..5470)
                     /locus_tag="VFQ61_09710"
     CDS             complement(4331..5470)
                     /locus_tag="VFQ61_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET9954772.1"
                     /translation="MGRFPFPQNRRLPGLYYPAGANAADARAAFERFRDEIVTSEGAG
                     GFLRTRRPDTPDGVVNSTVSEGIAYGMIIAVMFDDQALFDGLYGYAMTWFNENGLMDW
                     YIAPDGSKRLGVGGATDSDEDMAWALVMAHRQWGGRGALSETYLSHAKRQIDRVYELE
                     VDHDRWPDMLIPGDDWRGQCVFNPSYFAPHQYRVFGEVSGNVQGWQRVIDRGYEIIEN
                     SLNDREGNRDNGLVPAWCDAEGNPVEAFPGAMQNYQYDSARTPFRLGQDFAYHAEPRA
                     RAYLQRISTFFANIGADRIVDGYTLRGEAAPDPKAIRPNPGSAVFVGCAAVGAMHDPR
                     FASLVDGAYARVQRGDLLARSRYYNHCWTVMSLLMMTGNLFDLPE"
     gene            5627..6256
                     /locus_tag="VFQ61_09715"
     CDS             5627..6256
                     /locus_tag="VFQ61_09715"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HET9954773.1"
                     /translation="MSRVDEELAQALAESESNSASSGSASSGSGAADSAPEGQPVTRD
                     VSDGGSSSRPRSKGSLGLLIGLLVMGGGILTLVMTSFDNSQVYARQVDQVLNELNKLQ
                     GRTVRVEGTLVKGTLRRRDEPCEYRFSMSKGSAVLPVRYPQCIVPDTFRDMPDMDVKV
                     TAEGKLTAAGELEAHQIFAKCPSKYEMQDRANKGEKMPHQASGPSASLH"
     gene            6307..7344
                     /gene="hemE"
                     /locus_tag="VFQ61_09720"
     CDS             6307..7344
                     /gene="hemE"
                     /locus_tag="VFQ61_09720"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET9954774.1"
                     /translation="MWDRFLRACRREPVDCTPVWFMRQAGRYMAEYRAIREKHTLLEI
                     CRQPELATEVTLQPVRAFGVDAAILFADILLPLEPMGASFEFAAGEGPVIHHPIRTAE
                     DIVKLRLFEPEEGLPHVLEALRLIRRELDGKTPLIGFAGAPFTLASYLIEGGKSSNYA
                     RTKRMMYREPETFHALLSKLAEVVRVYLRAQVQAGAQAVQLFDSWVGSLCPLDYQTYI
                     QPHVRHILQDVMQTGVPVIHFGTDTSNLLELMRDAGGTVIGVDYRTPIDQAIARLGDG
                     VAVQGNLEPLLLGAPKELLSTRIDDVLQRVGARRGHIFNLGHGILPETPPDSIQFVTD
                     YVHERTQRSTH"
     gene            7508..7581
                     /locus_tag="VFQ61_09725"
     tRNA            7508..7581
                     /locus_tag="VFQ61_09725"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7540..7542,aa:Gln,seq:ttg)
     gene            7807..8505
                     /locus_tag="VFQ61_09730"
     CDS             7807..8505
                     /locus_tag="VFQ61_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9954775.1"
                     /translation="MIDLEDANESRFIERLQQRDERAFLELVQLYQVRVYKLVLRMVG
                     RRDEAEDMAQEVFVQVFKAIGTFRGESKLSTWVYRIAVNLCKNRLKYLTRRKNEAQDE
                     FEPHDARGDLAQAHAVTTADVARPDHLVEGYQLERIVERAITRMDPDFREILVLRDIE
                     DLSYEELSEITGLPDGTVKSRLHRARAMLKAAVDAELGGKHPAGAKADPKPEATRAKQ
                     EPAKPKLETKGRAR"
     gene            8502..8855
                     /locus_tag="VFQ61_09735"
     CDS             8502..8855
                     /locus_tag="VFQ61_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954776.1"
                     /translation="MNEIPKTNGGEARVSDLGLELPGERDLRSVLRGALGEPEAMPDL
                     TKGVQQKIRARSGGKFFADGWSTVKHPPINTYLVTSLMMLFVLGVIYALLAPLSGAPE
                     PARAPKPVQVLPGAR"
     gene            8936..11392
                     /locus_tag="VFQ61_09740"
     CDS             8936..11392
                     /locus_tag="VFQ61_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954777.1"
                     /translation="MLQISSLNDGWQFMAKTWLTEQSYGFSRFEWLPAHVPGHVHLDL
                     VANGVIAHPFEAQYELGCRWVDEEDFVYRRTFSFEPDPELPQTRLVFRGLDGVCSVFL
                     NDAQIAEHDNMFVPLELDVSDKLQRGKNQLRLEFQSAARVGRERRARYFAAEGIPEQV
                     LRFDERAFVRKAQYMFGWDWGPRLISCGLWADVELQRGRGRIVDVHVTQTHLPNGSVE
                     LEFRSELEGQGQVVHEVEGVPGRVADGEKLLIERPNLWWPAGLGPQFLYAIRSWLLPT
                     SAAPDASLADSALDCRSFRVGLRRVRLLQEPDALGSSFEFEVNGRKLWAVGANWIPDS
                     SFPAQVDRARVRAQVERARDMNMNMLRVWGGGLYESDDFYDACDELGVLVWQDFPFAC
                     SYYPDDESACVALRREASVNVKRLRNRASLALWCGNNENLTMWEDKWGEASRHPARYY
                     GERLYDEVLPALLRELDPNRPYIPSSPFAGVAEGKANAGGSGDQHYWDVWHGRGDWKH
                     YADSTGRFASEFGFAAAPSSASLRRLAPNVRDVLALPVRDAMLRWHDKTSKGYETFIG
                     YVEQHYPAARNAEEWIYYSQLNQRDALRFGIEHYRRSEFCKGSVIWQLNDCWPVQSWA
                     VLDDCFQYKAAAFELRRLYAPALVSLEMTGREACVWAILDNASEPLLDKLQVQVHSLL
                     DGRTLHEFEQKLELMPGDRRVITRVPLERFDPKTSIVSARFHGRTTFRLLDEPKNVRL
                     SAPRLNARFASGRLMIESDVPVVDLCVWDEAGSLVLKDNFVTVPSGGSCATLFAQGVP
                     GHLRARSLQGHHAIRVSSAR"
     gene            complement(11462..12235)
                     /locus_tag="VFQ61_09745"
     CDS             complement(11462..12235)
                     /locus_tag="VFQ61_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET9954778.1"
                     /translation="MNRPLRIEVAGQTDVGRKRSHNEDNFAIMAEYGLYVVADGMGGH
                     ASGEIASKMAVDTLHEFFARTADDPERTWPYKMDRTRGYEENRLVTGIKLCNLRIYEQ
                     AQRNSKQRGMGTTLVALFAVEDGVYIAHVGDSRVYRVRERQIEQLTEDHSLLNDYKKM
                     KRLTEEEIANFPHKNVIVRALGMKDSVKVDTRFEAPRSGDVMLLCSDGLCGPVSDERL
                     LQIILSSRDLPTATQRLIETANENGGPDNITCVLARWID"
     gene            complement(12453..13490)
                     /locus_tag="VFQ61_09750"
     CDS             complement(12453..13490)
                     /locus_tag="VFQ61_09750"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HET9954779.1"
                     /translation="MSLAISTTAVAQPLLTARPPTPSPEKPQGSEGGKPSPASGASGS
                     ASAATPASPPPVEAPPSTPKATAQPEGIVLVERAGRTIALGSVLAVDGRVLSSWSRVT
                     GDGPLTLRYSDGSQEPARVGHFDAAQDLALLVPETRRWQRGLTALRGFPPAGVALTTF
                     ALAPNRTLLAVGQHTTLVQENGRWLLKLSQAVKPEAWGGPLLDAQGKSAGLVVSCGND
                     TPNCAVVRPVDELRAFLKTLPVTAGFALPRLGLAGRAIDTGTVRGLLLSSIEPDGPAA
                     ALKLRTGETTADGDVLVAVGGAPVPTPNELRAALARHRAGSKVELLVFSKGEYRTVPV
                     QLAAYKKPVTR"
BASE COUNT         2469 a         4383 c         4408 g         2545 t
ORIGIN      
        1 gcttcgggcc cgttgcttgc gggaggatgc ggcagtcgcc acagtctgcc tgtggtccgc
       61 cgcttacctg tcgtgcaaac cgccgcctcc gaagacgccg aggcgatggc ccgccccgcc
      121 gcgcattggg tcgcgatcgg cgtggctttg atggtcgctt tgtttctgcc gctctcgatg
      181 ttgggcgtcg cggtggccca agctggattc aaagccccgg ccgcgggagc tctcttcgcg
      241 ctgtcggcgt cgagtgttgg ctccggcgcg gtgctcgggc gtttcggcct caaactgaaa
      301 gcccccggtg ccgcccttgc gggagcactc gggggcgctg ctttgctgct gttgtcccga
      361 ctcggcggaa accgagtatc aggcgggctc ttcttgttcg aggccggctt gttgatcggg
      421 ctgggggcgt tgttctgctt cgcgggtttt cgtttggggc acaggcgccg gccttgagct
      481 cggcgcctcg tcgccatctg catcttcgcg cagtggtggc tcactgcgcg gcccgcgctc
      541 ttccgacggt tcgctcttgg tcggttctcc cttcaagaac gcccgaaccg cgggatccgt
      601 tcgccacacg ttggccatta gccagccgag cgcgacgaac agcgtgacgg tggtgtcttg
      661 gaagtgacgg tcgagatcag gctccagtga gatccaataa ccagcgatgc agaggcccag
      721 cagcgggccc gcgctctgga gcagccgcag cggcaagccc tccactcgcg ccgcctcatc
      781 ctcgtgcgcc agcgcggcct gcgccttggt agcgccgctg cccagcaaca cgagcaagaa
      841 gatgaagtga ctgtagtagt tggaggggtt ccacatcacc tgcacgagtg gcagcgaaag
      901 taacatcgcc tggtaaagcg ggcgcttgcg gcaagcaatt gcgaccagca cgacgcacag
      961 cagtttggcc gaacccaaga tcaagaaccg ggcttgccca gcgcgcgcag agctcgagtc
     1021 cacgcccgcc accaaagcgc gcaagctgac ttcgttcacg cccacgtcgc ggttgagcaa
     1081 agtcaccttt tgccaccact gcagccatga ctcgaacgag tacaaggccg aggtgaccgc
     1141 aatcgcgacg gcaacacatg cggccgcgct caacaacacg cgcaccgcgt ctttatggcg
     1201 ctccaggata cgtttgatgg tcggcggctt gccttctttg cgccacgtct ccgccaccca
     1261 ccaaatcgcg ggcagcgcga ccccgaacag ggcaactacg gggaaggctc gaatcagcgc
     1321 cgccagcgcc atgaacacgc ccgccaggcg aaaacgatga gccttcaggc tgcacgccgc
     1381 gaagcccagg aaggccatcc aatcatggcg aagggttgcc cccgtccagt tcgtaccgaa
     1441 catgtataga tcgagcgcgc cgagcaccgt caacgccgcg agcgctggaa gcagcccgaa
     1501 ggttcgccac atcgcgatcc cgagcagcaa gaacaatgct gcgtcggcta ggcctgcgat
     1561 cgttagcacc ccatcgctcg cggacgtgtg ccccaagaag agccgcgcga agaacaccca
     1621 aaccggcgtc gcgttcgcgc cgtgatcggt gagtgtcccg atgaactcgg gccccatcac
     1681 ctcacggaag tacttcatgt cttggcgcaa ggcctcccag cgctccgggg tgaagcgctg
     1741 cttgatggcc aacatctcgt ctttgacgtc cgcgacgcga cgggctttgt gatctttgag
     1801 gctccgcact tccacgcgcg ccatcggctc gagcgagccg ccgtacacgt cttccgcgta
     1861 agtcagcacg ctcgccgggt agacaccgtc gtagcgcagc tcgtgaaagt acttcgcgaa
     1921 cggctgatac acgcgcatgt cataggtgtg tacgaacgtc ggccgccctt cctggtgatt
     1981 ccagaattgc gggcgaccaa ggttgtagaa cgccgcgagc gcgagcgcgg cgctgaacat
     2041 gaggctgccc gccacgatgc gacggtgcgc cggccaacgc ttggcgggga gcgccacgcg
     2101 gcacacggcg aacaacgcga tcgcagccgc agtagctcga gcgaacgcca cttcgcgcga
     2161 ggcgagtggc cacccgaact cgacactctg gtaggccaga taggcagcca ccgctacgcc
     2221 caagccgagg agcccaatca cccacgcctt gctgcgcgcg aaggtgccga acagcagcac
     2281 gaagaaggcg aatcccaaat acatgaactg cgttcggacg aggctgttct gcgcttgccc
     2341 cgtcgcgatc cgcagcccgg gcgggaactt cgcggggcga ctcgagaaca gctgcagctc
     2401 ggtgacggaa taagaacggt caccgccgcg cgcggtcaaa cgtacgtaac gcccgcgtgc
     2461 gttcagctgg tcggtgaagc gctcacggag cccagcctcg ggcgtagtcg gcgcggtcca
     2521 cagcggcgcg taagttttgc gatcctcgga gacggtcagc aagtactcgt cgttgttgtc
     2581 gccctgcaga taggcggctt tgatcgtctc gctctgcccg agatcgaact cgatgaaacc
     2641 ctgctcaggg ccgataatcg ccgctgcgga ggtgttccac tcgtcaccct ccggggcttt
     2701 cgcttcatcg gtcagggctc gcgcattctt catgccttga gagcgaatgg cgctcttgcc
     2761 ggcgagcaga ttggtttgcg caaagctcgg gctcgcatgc gtgatgaaca gggccgtaac
     2821 gcagcacacc agccatgcga acgatacgta ccggagcggg ccgcgcgctg ttcttgcact
     2881 tggctgtgta cctgcgctac gccgcgtact cgcgtcatgc cgcgtacgat gcccgtcgtt
     2941 agccgatctc atctctcgta ggtccttccg ccagagcccc ggggtggccc caacggcgcc
     3001 cactttactc aaagagcggg gtcggcgctc gctccgtcga gccgtttgcg gaagctcgcg
     3061 aaaccaagcg cgaagtccag ccggcgcgcg tcagccgtgc gaccgcggct gccccatgcg
     3121 cgacgagcgg cctgaaccac cccagtccaa gtcgatgtga gcctgcctca gccgcgttcc
     3181 ggcccatcgg gcagatcgcc cgtcgtgcta gtcaagcgag tcgcgctgtg cggttcgaac
     3241 tcggtcatgc gctcgcggag ggccgcgatc tcgcgctcgt gctcggaaat cagagtaaaa
     3301 atgaaggcat catggtctgc gacgagaaaa tcacgctcgc gtgccgcgga gcgcgaggtc
     3361 tcgaccacga cgtgctcgag gtgctggatg cggcgtttca gggcccggct cgggtcgtcg
     3421 ttctgcagtc gccccaatag cgaacccagt tcggagccga gccgctggtg agcatcgcga
     3481 atgctcgaga tgctcgcgct cagaaatttc acggttcggc tcagatcggt gagcgcagcc
     3541 tccgtgcggg cgtgggcggc gagggcagcg tcgaaacgcg acgccacatc ctccggcaac
     3601 tcgaggggac ccggcgggag cacgctgggc ctcactccgc tcaactcaga actgctctgc
     3661 tcagaactgc tctgctctgt ttggctcggc tccgaacggt tcggctccga acggctcggc
     3721 tcggctccga acggctcggt tcggcgcgaa atggagccgc tcgagtcagg gctatcgtca
     3781 cccagagttg cgggggttgc cttgggcatg gccatgcggt cacctcgtca atgacttcta
     3841 gtggcgcagc gccggctcga acaggcaaaa tcgagcatgc gacgccgctc caacgagtca
     3901 ctgctctagc agagcctcag cgtctcgggt gatgacggtt gagcaacact ggcgaatttt
     3961 ggagctggcg cacgcggtgg cccgatagcc cctgtttcgc gctgctttca gcgttcagtt
     4021 ttcttggggt tcagcgcttc gagttcacct ggagcaaagg taaaccgctg cgtcgaatct
     4081 tcctcggtga ggacgaactg cgccagcaac gtgttcgcca tgacatcacc tcgagcccgt
     4141 gctttttcga ggtaccccag tgccgtgccg aacgccacgg cacatttccg atcgctcttg
     4201 tttctcagcg ccgccgcgct ttcttcgagc agtcgcgcga cttcgcttcg ccactctgcc
     4261 aggtccatga gatgatgcta cggccgctcg agggggcgcg cgagccccct ctggcccccg
     4321 ttcgtgtcga tcactccggc aaatcgaaaa gattgcccgt catcatcagt agcgacatca
     4381 cggtccagca gtggttgtag tagcggctgc gcgccaacag gtcccctctt tgtacgcgcg
     4441 cgtacgcgcc atccaccaag ctcgcgaatc gcggatcgtg catcgcgccg accgcagcgc
     4501 agccgacgaa cacggctgag ccggggttgg gccgaattgc cttgggatcg ggagcggctt
     4561 cgcctcgtag cgtgtagccg tccacgattc gatcggcgcc aatattggca aagaacgtcg
     4621 aaatgcgctg caagtacgcg cgggcacgcg gctcggcgtg ataggcgaag tcctgcccca
     4681 accgaaacgg cgtgcgcgcg gagtcgtact gatagttctg catcgcccca ggaaacgctt
     4741 ccaccgggtt gccctccgcg tcgcaccatg ccggcacgag gccgttatca cggtttccct
     4801 ctcgatcgtt caggctgttc tcgatgattt cgtaacctcg atcgatcaca cgctgccacc
     4861 cctgcacatt gcccgacacc tccccaaaca cgcggtactg atggggggcg aagtacgatg
     4921 ggttgaacac acattgcccc cgccagtcgt cgcccgggat cagcatgtcg ggccagcgat
     4981 cgtgatccac ctcgagctcg tacacgcgat cgatttggcg tttggcgtga gacaaatagg
     5041 tctccgagag cgcgccgcgg ccaccccact ggcgatgcgc catcacgagg gcccaagcca
     5101 tgtcctcgtc cgagtccgtg gccccgccca cgccgagccg cttggagcca tccggagcga
     5161 tgtaccaatc cattagcccg ttctcgttga accaggtcat cgcgtagccg taaaggccgt
     5221 cgaatagagc ctgatcgtcg aacatcactg cgatgatcat gccgtaggcg ataccctccg
     5281 agacggtcga gttcaccacc ccgtcgggtg tgtctgggcg ccgcgtgcgc agaaagccgc
     5341 cggctccttc gctcgttacg atctcgtcac ggaagcgctc gaaggccgcg cgcgcatcgg
     5401 cggcattggc tccggctgga tagtaaaggc ctgggaggcg gcggttttga gggaagggga
     5461 agcgacccat ggcccgcttc taccgcatcg agcacatgcg tgcgggcaag ttccggcgca
     5521 tttgccgcaa acatagaaag atccgtgggc cgcgtggaca acattcgcgc ccgtcttccg
     5581 gccgccggtg gacccaactg ggggtcggag ctaggctttc gccaacatga gtcgcgtcga
     5641 cgaagagctc gcccaggccc ttgctgagtc ggagtccaat tctgcatctt ccggctctgc
     5701 ctcctctggc tcaggagccg ccgattccgc gcccgaaggc cagcccgtca cgcgtgacgt
     5761 aagtgacggc ggctcgagtt cgcgaccgcg ctcgaaggga agtttgggcc tcttgattgg
     5821 cctgttggtg atgggcggcg gcatcctcac tttggtgatg acgagcttcg acaactcaca
     5881 ggtctatgcg cgccaggtgg accaggtatt gaacgagctg aacaagctcc aggggcgcac
     5941 ggtgcgcgtc gaggggaccc tcgtaaaggg cacgcttcgg cgtcgtgacg aaccctgcga
     6001 atatcgcttc agcatgtcga agggcagcgc cgtgctaccg gtgcgctacc cgcagtgcat
     6061 cgtgcccgat acctttcgag acatgcccga catggacgtc aaagtgacgg cggaaggcaa
     6121 attgacggcc gcaggtgagc tcgaagcgca ccagatcttc gccaagtgcc cgtcgaaata
     6181 cgaaatgcag gaccgcgcga acaagggcga gaagatgccg catcaagcga gcgggccgtc
     6241 ggcatcgctg cattgagccg cgacgaagct cttccgttcg gacgtttcct cacgtaacca
     6301 gtagccatgt gggatcgttt tctgcgcgcc tgccggcgtg agcccgtcga ctgcactccg
     6361 gtttggttca tgcgccaggc tggccgctac atggccgagt accgggccat ccgtgaaaaa
     6421 cacacgctac tcgagatctg tcggcagcct gagctcgcga ccgaagtaac gctccaaccg
     6481 gtacgcgcct tcggcgtgga tgccgccatc ttgttcgcgg acatcttgtt gccgctcgag
     6541 cccatgggcg cttcattcga gtttgcggcg ggtgaggggc cagtcattca tcacccgatc
     6601 cgcaccgccg aagatatcgt gaagttgcgg ctgttcgaac cggaggaggg gttgcctcac
     6661 gtgctggagg cgttgcgcct gatccgacgt gagctcgatg gtaagacgcc gttgattggc
     6721 ttcgcaggcg caccgttcac tctcgcgagt tacttgatcg agggcgggaa gagctcgaat
     6781 tacgcgcgca ccaagcgcat gatgtaccga gaacccgaga cgttccatgc gctgctctcg
     6841 aagctcgccg aggtcgtgcg ggtttacttg cgcgcgcagg tacaggcggg ggctcaagcc
     6901 gtccagctgt tcgactcctg ggtggggtcg ctctgcccgc tcgactatca gacgtatatc
     6961 cagccccacg tccggcacat cctgcaggac gtgatgcaga ccggcgtacc cgtcatccat
     7021 ttcgggaccg acaccagcaa tctgctcgag ttgatgcgtg acgccggagg gacggtgatt
     7081 ggcgtcgact atcgaacgcc gatcgaccaa gccatcgccc gcttgggcga cggggtagcg
     7141 gtccaaggca acttggagcc gcttctgctc ggcgcaccca aggagctgct cagcactcgc
     7201 atcgacgacg tgctccagcg cgtcggcgct cggcgtggtc acattttcaa cctgggccat
     7261 ggcattctcc cggagacacc gccggactcc atccagttcg ttaccgatta cgtccacgag
     7321 cggactcagc gctcgacgca ttgacgcgat atcttcgatt ggggagccgg gtgaccggtc
     7381 gaagtgggct gggccgtcgg ccacgcgggc agcgcaaaat cgtgtttcga tccgcttgtc
     7441 gccgaacgaa acccgactag tctgcgccca ccgattggtc gcgctcgaat gagcgggcca
     7501 tgcctgatcc ggcgtcgtct aaaggcagga caagggactt tgaatccctg aatcgtggtt
     7561 cgaatccatg cgccggaacc acccacactc gcggagatcc actgacccgc gcgacgcccg
     7621 agcctctcgg gagcgcgcaa cgcaggcaaa cggatcgatc cgacggagcc caagtgaagc
     7681 tgcgcgaggc aatggaagga gccggggcag cggccccggc agccgaggcg gggccgagca
     7741 ctatggagca tccgcccgcg atcgggaact tgcgtcccga gccgctgtcg cagactcttc
     7801 gagaacgtga tcgatcttga ggacgcgaac gagagccgct tcatcgagcg gctgcagcag
     7861 cgcgacgagc gagcgtttct cgagctcgtg cagctgtacc aggtgcgcgt ctacaagctg
     7921 gtattgcgaa tggtggggcg tcgagacgaa gccgaggaca tggcccaaga ggtcttcgtc
     7981 caagtcttca aggccatcgg tacctttcgt ggcgagtcca agctcagcac atgggtgtac
     8041 cggattgcgg tcaatctctg caaaaaccgc ctgaaatacc tgacccggcg caagaacgaa
     8101 gcccaagacg agttcgagcc acatgacgcg cgcggggatc tcgcgcaagc tcacgcggtc
     8161 accacggccg acgtggcgcg ccctgatcac ttggtggagg gttaccaact cgagcgcatc
     8221 gtggagcgcg cgatcacgcg aatggaccca gatttccgag agatcctggt gctgcgtgac
     8281 atcgaagatc taagttacga ggagctctcc gagatcaccg gtttgcctga tggcaccgtg
     8341 aagagtcgct tgcatcgggc gcgcgcgatg ctcaaagccg cggtggatgc cgaactcgga
     8401 ggaaagcatc ccgccggagc caaggcggat ccgaagccgg aagccacgag ggccaagcag
     8461 gaaccggcca agcccaagtt ggaaaccaaa ggtagagcgc gatgaacgaa atccccaaaa
     8521 ccaacggcgg cgaggcccgc gtttcggatc tcggcctcga gctaccgggt gaacgggatc
     8581 tccgttcggt gttgcgcggc gcgctcggtg agccggaagc aatgcccgat ttgacgaagg
     8641 gcgtgcagca gaagatccgc gcgcgaagtg ggggcaagtt cttcgccgac ggctggagca
     8701 ccgtcaagca tccccccatc aacacctacc tggtgacgag cctgatgatg ctgttcgtgc
     8761 tcggcgtgat ttatgccctg ctcgccccgc tctcgggtgc gcccgaaccg gcgcgcgcgc
     8821 cgaagccggt tcaagtgctt cccggtgcgc gctgagcgac cggagagctg tagctcgggt
     8881 tccagttcga attcgtttgt cgtacagcgc ggggttctag gctaatttgc ggcgcgtgct
     8941 gcaaatctca tcgttgaacg acggctggca attcatggcc aaaacttggc tcacggagca
     9001 gagctacggt ttcagccgct tcgaatggtt accggcacat gtgccgggcc acgtgcatct
     9061 ggatctcgtc gcgaatggcg tgatcgccca tccgttcgag gcccagtacg agctcggctg
     9121 tcgttgggta gacgaagagg acttcgtgta tcggcgtacc ttctctttcg agcccgatcc
     9181 cgagttgccc cagacacgct tggtatttcg agggctggac ggtgtgtgct ccgtgttctt
     9241 gaacgacgcc cagatcgccg agcacgacaa catgttcgtg ccgctcgagc tcgacgtcag
     9301 cgacaagctg cagcgcggta agaatcaact gcgcctggag tttcaatccg cggcgcgggt
     9361 tgggcgcgag cgccgggcgc gctatttcgc cgccgaaggc attcccgagc aggtgctgcg
     9421 attcgacgag cgcgccttcg tacgcaaggc gcaatacatg ttcggttggg actgggggcc
     9481 acggctgatt tcctgtggct tgtgggcaga cgtcgagctg caacgcggcc gcggacgtat
     9541 cgtcgacgta cacgtcaccc aaacccactt acccaatggc agcgtggaac tcgaattccg
     9601 ttcggagctc gagggccagg gccaggtcgt gcacgaagtc gagggcgtgc cgggccgggt
     9661 tgccgacggt gagaagctgc tcatcgagcg ccctaacttg tggtggcccg ccgggctcgg
     9721 tccgcagttt ctgtatgcaa tccggagctg gctacttccg acttcggccg cgcccgacgc
     9781 atctcttgcg gactcggcgc tggattgccg ctccttccgc gtaggtttgc gccgcgttcg
     9841 cttgctccaa gagcctgacg cgctgggctc gagcttcgag ttcgaggtca acgggcgaaa
     9901 gctgtgggcc gtgggtgcga actggattcc agactcgtcg ttccccgcgc aagtggaccg
     9961 cgcccgcgtg cgcgcccagg tggagcgtgc gcgcgacatg aacatgaaca tgctccgggt
    10021 ttggggtggt gggctgtacg agagcgacga tttttacgac gcctgcgatg agctcggcgt
    10081 cttggtttgg caggatttcc cgttcgcgtg cagttactat cccgacgacg agtccgcttg
    10141 cgtggcactg cgtcgtgaag ccagcgtcaa cgtgaagcgc ctccgtaacc gcgcgagtct
    10201 tgccctatgg tgcgggaaca acgagaacct caccatgtgg gaggacaagt ggggcgaggc
    10261 ttcgcgtcat ccagcccgtt attatgggga acgcctttac gatgaagtgc tccccgcgtt
    10321 acttcgcgag ctcgacccga accgccctta cattccgagc tcacctttcg ccggggtcgc
    10381 cgaaggcaaa gcgaacgccg gcggatccgg tgatcagcac tattgggatg tctggcatgg
    10441 ccggggtgac tggaagcact acgcggactc caccgggcgc ttcgcatctg aattcgggtt
    10501 cgcggcggcg ccatcctcgg ccagcctgcg ccggctcgcg ccgaacgtac gcgacgtgct
    10561 cgcgttgccg gtgcgtgatg ccatgttgcg ttggcacgac aagaccagca aaggttatga
    10621 aaccttcatc ggctacgtgg agcagcatta cccagcggct cgcaacgcag aagagtggat
    10681 ttactactcg cagctgaatc agcgggacgc tctgcgcttc gggatcgagc actatcgtcg
    10741 ttcggagttc tgcaagggct cggtgatttg gcagctgaac gactgctggc cggtgcagag
    10801 ctgggcggtg ctcgacgatt gctttcagta caaagccgcc gccttcgagc tgcggcgcct
    10861 gtacgcgccg gcgttggtgt cactcgagat gactgggcgt gaagcgtgcg tttgggccat
    10921 cctggataac gcgagcgagc cgctgctcga caagttgcag gtccaagtcc actcgctgct
    10981 ggatggccgc acgcttcacg agttcgaaca gaagctggag ctcatgcccg gcgaccggcg
    11041 tgtgatcacg cgcgtgccgc tcgaacggtt cgatccgaag acgagcatcg tgagcgcgcg
    11101 tttccacggc cgcaccacgt ttcgactcct ggacgagccc aagaacgtca ggctatcggc
    11161 cccacgcttg aacgcgcgtt tcgcgagcgg gcgtctgatg atcgagagcg atgtgcccgt
    11221 cgtcgatcta tgcgtctggg acgaagcagg cagcttggtg ctcaaggaca acttcgtgac
    11281 cgtgccctcg gggggtagct gcgcgacgct gtttgctcag ggcgtgcccg gacacctgcg
    11341 cgcccggagc ctccaaggtc atcacgccat ccgcgtgagc tcggcgcgct gagatttgcc
    11401 gacagtagcg ccggcatgcg agtcgacgag atcgacgcat gccgttcgca tacaacgacc
    11461 gttagtcgat ccagcgcgcg agcacgcacg taatgttgtc gggtccacca ttctcgttgg
    11521 cggtctcgat caacctttgc gtggcggtcg gcaagtcacg cgagctcagg atgatctgga
    11581 gcagtcgctc gtccgagacg ggcccgcaga ggccgtcact gcaaagcagc atcacgtcac
    11641 cactgcgcgg ggcctcgaag cgtgtgtcca ccttcacgct gtccttcatg ccgagcgcgc
    11701 ggacgatcac gttcttgtgc gggaagttcg cgatctcttc ctcggtcagc cgcttcatct
    11761 tcttgtagtc gttgagcaag ctgtggtctt ccgtgagctg ttcgatctgc cgctcccgga
    11821 cgcggtacac gcggctgtcg ccgacgtgcg cgatgtaaac gccgtcttcc actgcgaaca
    11881 acgcgaccag cgtggtgccc atgccgcgct gcttggagtt gcgctgcgcc tgctcgtaga
    11941 tgcgcagatt gcagagcttg atgccagtta cgagtcggtt ttcctcgtag ccccgcgtac
    12001 gatccatctt gtatggccac gtacgctccg ggtcatccgc cgtgcgcgcg aagaactcgt
    12061 gaagcgtgtc cacggccatc ttcgaggcga tttctcccga tgcgtggcca cccataccgt
    12121 cggcaacgac atacagcccg tactccgcca tgatggcgaa gttgtcttcg ttgtggctgc
    12181 gcttgcgacc gacgtccgtt tggccggcga cttctatcct gagaggccgg ttcattgttg
    12241 gctggaggtg ccttggggcg ttgcttgcct gaagggcttg ctggggtggg gcaccaattg
    12301 ttctattcag ccgacttcac gcggacttgt caattcacgg tgcggagact ggctggccgt
    12361 tctgtgtgac ctggagccgc gctctggagt gcgatagctg cgtctctgcg aaccagaatt
    12421 ccctatcgtc cgccatgccc cggagcaacg gctcagcgag tcacgggttt cttgtacgcc
    12481 gcgagctgca ccggcaccgt tcggtactcg cctttcgaga agacgagcag ctccaccttg
    12541 gaacctgccc gatgtcgggc caaggctgca cgcagttcat tgggcgtcgg gaccggagcg
    12601 ccaccaaccg ctacgagcac gtctccatcc gcggtcgtct cgccggtgcg cagcttcaac
    12661 gccgccgcgg ggccgtcggg ttcgatgctc gacaagagta gccctcgcac cgtaccggta
    12721 tcgatggccc tgcccgctag tccgagtcgg ggtagcgcaa acccggccgt gaccggcagc
    12781 gtcttcaaaa acgcgcggag ttcatcgacg ggcctcacga ccgcgcaatt gggagtgtcg
    12841 ttgccgcaac ttacgaccag ccccgcggac ttgccctgcg cgtcgagcaa gggcccgccc
    12901 cacgcttcgg gtttcaccgc ctgagatagc ttgagcagcc agcgtccatt ttcttgaacc
    12961 agggtggtgt gctgtccgac cgcaagcagc gtgcgattcg gagcgagtgc gaaggtcgtc
    13021 agcgccacgc cggctggggg aaagccgcgt aaggccgtca aaccacgttg ccaacgtcga
    13081 gtttcgggca cgagcaacgc tagatcttgc gcggcatcga agtggcctac gcgtgccggc
    13141 tcttggcttc cgtccgagta gcggagcgtg agcggaccgt cccccgtgac gcgcgaccag
    13201 gagctcaaca cgcggccatc gaccgccagc acgctaccca gcgcaatggt gcgacctgcc
    13261 cgctccacga gcacgatgcc ttcgggttgt gcagtggctt tgggcgttga cgggggcgcc
    13321 tcgaccggcg gcggggaggc aggcgttgct gcgctggcgg agccgctagc cccgctagca
    13381 gggctcggct taccgccctc tgagccttgg ggcttctcgg ggctaggcgt gggcggacga
    13441 gccgtaagta gcggctgagc gacggccgtc gtggaaattg cgagactcac gctgctggcg
    13501 agagcgagac gaagggctga ggtccgccaa gaccggcggc tcgagtgaag atggttccgc
    13561 cacatgcccg ggcagtttaa cccggatccg gcccgaaaag ccgagccccc ctggaagccc
    13621 caaaaaagca gccaaaacca gcgcgtgccg gtgttggggc ggaggagtgt agctaaccgg
    13681 cctctgagtg agcggcgctg gtgcgtggcc ggctcagacg gctgagtcca ccgctcgctt
    13741 caggacttcg attccgctcg ggcgcgccac gatcacgacg tccgcacgac cgcaaaacaa
    13801 tcccg
//
LOCUS       DASTKU010000270         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_274246, whole genome shotgun sequence.
ACCESSION   DASTKU010000270 DASTKU010000000
VERSION     DASTKU010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274246"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFQ61_09755"
     rRNA            complement(<1..423)
                     /locus_tag="VFQ61_09755"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1225..1872)
                     /locus_tag="VFQ61_09760"
     CDS             complement(1225..1872)
                     /locus_tag="VFQ61_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954780.1"
                     /translation="MISRRLFGRRDQAPSSFAPTLGRLCSSAGVVGAALVDADGEAVD
                     YAGRVDSFDIRVSAAEWRIILARLGRSSFGPWRGTHQLMVRGVNKSFGAVLLPEGYAL
                     VVQLLPRCFRVSPRALSEAVREICENAGLELPQNAAGQWRRVQVNESPAPQRRPESVW
                     VNRKWLPVEVLGRWVDPELLPNEIGYRVRLLGGEEITLVREPLAQWFADPGAALQ"
     gene            complement(1869..2783)
                     /locus_tag="VFQ61_09765"
     CDS             complement(1869..2783)
                     /locus_tag="VFQ61_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9954781.1"
                     /translation="MPRADRALEWLSRLLCGLSVACSPAARNDAAASGSGPASVHVDR
                     RIAAQLEQGLGVAPERALELATEDALLATELERTAPQRAHALERLALARALERSLSAH
                     AVERGPATDAEVAELTAERWWALDRPRLVQVQHAVVLTERSDAEARALCERIRDAVRD
                     VHGEAAFAAAVKRVSSGKFKVKVEKLDPVALDGRVVDPDRPPPSNAPPQAYALEVARA
                     AQQLQQPGEVSPVVQSPFGYHVLRLLRVIEPHQPSLEERRRLLTPEIDQRRALTEQNE
                     LLARVRREAAPDQARAALTLMGRLSVAP"
     gene            complement(2770..>2999)
                     /locus_tag="VFQ61_09770"
     CDS             complement(2770..>2999)
                     /locus_tag="VFQ61_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954782.1"
                     /translation="WFGCSERRFGVACALVMARSADPRAALLILFASLALAYGIQQLS
                     VASEIRGLYWLIAGGLGLRAANDRERFLASR"
BASE COUNT          529 a          970 c          983 g          517 t
ORIGIN      
        1 ttcatcgctc acgcggcgtc gctccgtcag gctttcgccc attgcggaag attcccgact
       61 gctgcctccc gtaggagtct ggaccgtgtc tcagttccag tgtggctgtt catcctctca
      121 gaccagctac ccgtctttgc cttggtgggc cgttacccct ccaactagct gatgggccgc
      181 aggctcatcc ccaagcgcga gctttcatga agaggcccgc tttggacaca atctcgaaaa
      241 accgtgccgt catgctgtat tagctctcct ttcggagagt tgtccaccac tcgggggttg
      301 attacctacg tgttactcac ccgtgcgccg ctttaccggg gccgaagccc tttctcgctc
      361 gacttgcatg tcttaggcgc gccgctaacg ttcgttctga gccaggatca aactctccag
      421 ttaaatcctg tagtcaatcg aggttcgcct gcacattcgg gttaggagtg tgcaagtgag
      481 gccactgagc tcgataggga gtcgctcggt tttcagcgag tgaatccaag ccctgaagat
      541 tgctccacag ggaaacccgg gcgccggtaa accgtgccag gttgtatcga gcgagtaagc
      601 ttcggtatcc tgcgtggcta gcgtttccgc cagtccacga ggtggcaacc tcgacgctta
      661 atgctctttg cgattcatcg tctcacgccg gctcggatct tcttttgcgg aagtcacctt
      721 ctcacgagga agctggccgg tgcggacagg gacccgcgat gtctatcgcg ggaataggac
      781 tcagttttca aggaccggcg gaaggcgttg ctgccgtccg aaggggaggc ggaaggtagg
      841 cggaggcacc cgccttgtca aactcgtttt taaaaaatct gagcgagagc attcgattcg
      901 gcgcagacgg ggagtagctg ctcatactgt caggagcggc gggcgacgag ccggccgcca
      961 ggggggcgcg gcggcgacgg ggcgcaacgg gcgcagcgcg tggcgtgtgc acacgcgcag
     1021 ccgcgaattc cttcacggac tcgagcgcgt ctcgtgcagc gtctgggcga cccgctccgg
     1081 cgctcgcgcc ggcgcttggg cgtggcgagg tcggactcag agtctgggaa cgggcaacgg
     1141 cgcatcgacg cgcgagacgc tcagccgttg ttcgagctgg cgaagctcct cagcggggtc
     1201 gcgcacacgc agctgccgca ctcgtcattg tagagcggcg ccggggtcgg cgaaccactg
     1261 cgcgaggggc tcacgaacca gggtgatctc ttctccgccc aaaaggcgca cgcggtagcc
     1321 gatttcattc ggcaagagct ctggatcgac ccagcgcccc aggacctcca ccggcagcca
     1381 cttgcgattc acccacacgg actcgggtcg gcgctgcggc gcgggtgatt cattgacctg
     1441 gacgcgacgc cactgcccgg ccgcgttctg cggcagctcc aggccggcgt tctcgcagat
     1501 ctcgcggact gcctccgata gtgctcgggg cgaaacgcgg aagcagcggg gcaagagctg
     1561 caccacgagg gcgtatccct cgggcaacag gaccgcacca aagctcttgt ttactccgcg
     1621 caccatcaac tgatgcgtgc ctcgccaggg cccgaagctc gaacgcccga gccgagccag
     1681 aatgatgcgc cattccgctg ctgaaacacg gatgtcgaac gaatccaccc gtccggcata
     1741 gtcgaccgcc tcgccatcgg cgtcgaccag cgcggctcca accacgcccg cggaggagca
     1801 caagcgcccg agtgtgggcg caaatgacga gggcgcttgg tcgcgccgac cgaacagcct
     1861 tcgcgagatc atggcgcaac cgacagccgc cccatgagcg tgagcgcggc acgcgcttgg
     1921 tcgggcgccg cttcgcgacg cacgcgcgcc aacagctcgt tctgctcggt gagcgcgcga
     1981 cgctgatcga tttcgggggt gagcaagcgc cgtcgttctt ccaagctggg ctggtgtggc
     2041 tcgatgacgc gcagcagccg taatacgtga tagccaaatg ggctctgcac gaccgggctc
     2101 acttcgccag gttgctgcaa ctgctgtgcg gcacgcgcga cttcgagagc gtaagcttgt
     2161 gggggagcat tcgagggcgg aggccggtcg ggatccacga cgcggccatc cagggcgacg
     2221 gggtcgagct tttcgacttt gaccttgaac tttccagatg agacgcgctt gacggcagca
     2281 gcgaacgctg cctcgccatg cacatcgcgc accgcatcgc gaatgcgttc gcagagcgcg
     2341 cgggcttcgg cgtcggatcg ttcggtcaag acgacggcat gctggacctg tacgagacga
     2401 ggacggtcga gagcccacca gcgctctgcc gtcagctccg ccacctccgc gtccgtggcg
     2461 ggtccgcgct cgaccgcgtg tgcagacaag ctccgttcga gggcgcgcgc gagggccagg
     2521 cgctcgagcg cgtgcgcacg ctgcggcgcc gtgcgctcga gctccgtggc cagcagggcg
     2581 tcctcggtcg cgagctccag cgcccgttcg ggtgcaacgc cgaggccttg ctcgagttgc
     2641 gcggcgatgc ggcgatcgac gtgcacggaa gcggggccgc tcccactcgc cgctgcgtcg
     2701 ttgcgtgcgg ctggactaca ggccacggac agcccgcaca gcaggcggct cagccactcg
     2761 agagcgcgat cagcgcgagg caaggaagcg ctcccgatcg ttggccgcgc gcaagcccag
     2821 gcctcccgcg atcagccaat acagccctcg aatttcggat gcgaccgaga gttgctggat
     2881 gccgtacgcc aatgccagcg aggcgaagag gatgagaagt gcggctcgcg ggtccgcgct
     2941 gcgcgccatg accagagcgc aggctacacc gaaacggcgt tcagagcagc caaaccaac
//
LOCUS       DASTKU010000271         6644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_52830,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000271 DASTKU010000000
VERSION     DASTKU010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52830"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFQ61_09775"
     CDS             complement(<1..693)
                     /locus_tag="VFQ61_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954783.1"
                     /translation="MNSPNTSRRPPHNSTQPIAVAGSHAGAARAAQTAEFEPQPRPSR
                     RARLGSWKHVLSVSANLLVGLALLSPNDALAKPKSKPKLPQSAALRLVRAERAAGNSG
                     SEDDASRAGDVTEHPVDPGLEQLGLPPDAPRPATGDENFGDTAGATPYHFKVLLQNRF
                     RRTAVDISPELHDLILNDFSGSTTGAASFEQRIRDDARENDGLGLQRAFLRADAELGH
                     SFSMKLLVDFAEL"
     gene            complement(1380..2342)
                     /locus_tag="VFQ61_09780"
     CDS             complement(1380..2342)
                     /locus_tag="VFQ61_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954784.1"
                     /translation="MASRPDSVNNQAMPARVASQRTRSSSALASRWSWLAFSCVLGVA
                     LQCTSETEVGRDWARAGAGGSTSAGGATSGAPSATGGEAPFVCTPTTCSGKSYACGNC
                     EDDDEDGLIDALDPECLGPCDDTEQSISSGPHTETNGQCRSDCYFDRNAGAGGDRCEW
                     SQRCDPLSVAPGYPPSGDPGCAYDAAASIQGQSCTALQTAQSETCLNNCLPLTPNGCD
                     CFGCCELPARSNNWVFVGSASGGVETCNVDKARDPAACHPCTPVRACLNPCERCEACV
                     GGARPALNCTPEAACEEGQTPCGFEQGCSGAYYCITGCCIAVPR"
     gene            complement(2314..3093)
                     /locus_tag="VFQ61_09785"
     CDS             complement(2314..3093)
                     /locus_tag="VFQ61_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954785.1"
                     /translation="MSEFRDPPRWVLAPHTPSSEGLQRLFQEAERDVPNDSQLAALES
                     RLRDLMGSGVAGPQALEPQPEPPLHATRAPLSRAGKLGLVGLAVGVVAAGVAVVVLVN
                     RKAPDPTPPVLPAPSRTITVPSQPAPLPPSPPAVDSSLVPPSPTPSSAEPAPAQEAEP
                     KRAQPPRVAPNTPSAASEAELLERARKALAQDPTAALALTRTHEVRFASGLLRQEREV
                     IAIEALRRLGRQAEASARAERFSKTYPGSAHRRGVETGLGQ"
     gene            complement(3090..3611)
                     /locus_tag="VFQ61_09790"
     CDS             complement(3090..3611)
                     /locus_tag="VFQ61_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9954786.1"
                     /translation="MSSSPETPDFDSVFKANARYIWRSLRYLGVREADLEDVCQEVFV
                     TAFRKLPSFEGRSSVRTWLYGICLRVASDFRRRAHVVRERPYAEPFAESPTLGSEPQG
                     NLEARHLLTSLLSQLDDDKREVLVLYEVEGFTMKEVAEILGCPQQTAYSRLYAAREKL
                     LTLAQERGSKAAP"
     gene            complement(3724..4785)
                     /locus_tag="VFQ61_09795"
     CDS             complement(3724..4785)
                     /locus_tag="VFQ61_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954787.1"
                     /translation="MTGALLGIALPSQALGAPRVHLQLEDASDSTGCISAPDLEKQVE
                     ARLGRRVFTDRPGELRLALKLARIGGTWSAKLTLSDPMGILGQRELRSRGKHCSSLDD
                     SLALVVALLVDTPTAPPSEPVPEAKPPKGPEAPSISGGKTAADPRQSADRARAAPSRP
                     TTGEPTPLTLPADTPARREPWQFEIGAHLLLTAGALPGLAPGGELAFSASAPRAPEVR
                     LSLDAYRSESASVVDRDAGARFFLARVGLEVCGNVLTGERWALRTCAGQHLGRLSAAG
                     YGFDQNVSRARVLDAVGGSVEGTLRASERLKFALAARVELPLQREKFIWRNETGTTDS
                     VFEQAWAFLAVKMGLRWSP"
     gene            4889..5869
                     /locus_tag="VFQ61_09800"
     CDS             4889..5869
                     /locus_tag="VFQ61_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954788.1"
                     /translation="MHSKLTLHTWQRPLWALLLLSPLGLGALGCSGRTQSDPGDMPAG
                     NSGLPGAGANSGGAAGAGARPGTSGGNGSGASAGSGASAGSGASAGDSTGAGTGSTGG
                     VATTQCNNGLDDDGDGLADGFDAECTGALDNDEGSFATGIPGDNRDPKWQDCFFDGNS
                     GAGDDHCRYSTGCLTGDLPSDSSQCQLAQSCLGFCAPLTPNGCDCFGCCTVVLGSGKE
                     IDVQIGSSCSLDKVMDAAACPVCQKSTQCQNDCGKCDLCAGKTAADLDPSCTPETPPP
                     PPGTEPPPPSTEPPPPSYTCEPGQAKCGPGLPACSGSEYCAQGCCILSIR"
     gene            complement(5960..>6644)
                     /locus_tag="VFQ61_09805"
     CDS             complement(5960..>6644)
                     /locus_tag="VFQ61_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9954789.1"
                     /translation="RNEVRVSTELDDQGRIVISIADTGSGMPPEVQRRLFTPFYSTKP
                     PGSGTGLGLAICHRIVSSLGGDISFESQLGKGTRFVVALPSAEGEVSPRSHGSNSRTS
                     FSNPARRSRVLVIDDDQAVGALVRRALLNDYDVVVVNSAEHALDMLQRGEQFDVVLCD
                     VMMPQMTGAEFYEAVLRANLSIENRIIFMTGGAFTANAREFLDRVNLPFLEKPFDLST
                     LRELVASKL"
BASE COUNT         1179 a         2108 c         2195 g         1162 t
ORIGIN      
        1 cagctcggcg aagtcgacca agagcttcat cgagaaggaa tgacccaact ccgcgtcggc
       61 tctcaagaac gcgcgctgca ggcccaaccc gtcgttctcg cgcgcgtcgt cgcggatgcg
      121 ctgttcgaag ctggccgcgc ccgtcgtcga acccgagaag tcgttcagga tcaagtcatg
      181 aagctcgggg ctgatgtcga cggccgtgcg ccgaaaccgg ttctggagca agaccttgaa
      241 gtgatacggc gttgcacccg cagtgtcgcc gaagttctcg tctccggtgg ctgggcgcgg
      301 tgcgtccggc ggcaggccca gttgctccag accagggtct accggatgct ccgtcacgtc
      361 tcccgcgcgg ctggcgtcgt cttcgctacc ggagtttccc gcggctcgct cggcacggac
      421 caagcgcagc gcagcgctct gcgggagctt gggttttgac ttcggcttgg ccagggcatc
      481 attcggtgac agcaaagcca acccgaccag caagttggcg ctgacggaga gtacgtgttt
      541 ccaggagcct agacgagccc ggcgcgacgg cctcggttgc ggttcgaatt cagccgtttg
      601 cgcggcccgc gcggccccgg cgtggctccc ggctaccgcg atgggttgag tactgttgtg
      661 aggggggcgc ctggaagtgt tcggagaatt cacgacgttc aactcagcgg gaggagtcgc
      721 atagcatgtc gaagacgacg tcgtgaaaga atgacgacgt gcgacaacgg cgttagacgc
      781 tgctgatgaa agatcgaagc gagctcggat tcaggcgcgc gagcactcac gatgtggtga
      841 taaatgtttt catggtgtga gctgtcaatg attaatgttt aaacctgaag tcgacgagta
      901 cgagcgcacc acgaaagacg ggccgcggat ctcgaggcgc ctccttcgga ttaggaggcg
      961 cgaattcacg ttgaagcggc aatcagttgg ggcgggcgcg agagcaactc tggatcacgg
     1021 gacggcaact ccggtgtgcc cgttttcggt ctccaccgag ctccgttgtc ggagttcctg
     1081 ggttcgatct ggtgttgccc agtcctcgcc gcgggcactt cgtccctgcg acttagggca
     1141 gggctgtttg ggatcacggc agctcttcga ggccgcgagt gcagccggct gtgcgtgggg
     1201 agagactcca aagcaggcac tcggtgtcag gcacgagaac gccgtcagcg tccgagcgca
     1261 gacgcgcgcg agccatgcca acgagatagc gcgatcagga ttgacgatca tcgttgatcg
     1321 tatgtctcga ggcccgcgct tccgtttacg agatcgacca acgcatcaag cactcgcact
     1381 caacgcggca ctgcgatgca acagcccgtg atgcagtagt aggcaccgct gcagccctgc
     1441 tcgaacccgc acggtgtttg cccttcctca cacgcggcct ccggcgtgca gttcagcgct
     1501 ggccgcgctc cacccacaca agcttcgcag cgctcgcagg ggttcaagca ggctcgcacc
     1561 ggcgtgcagg gatggcaggc agccggatcg cgcgctttgt cgacgttgca ggtctccact
     1621 ccgccgctcg ccgagcccac gaacacccaa ttgttgctgc gcgcgggcag ctcgcaacag
     1681 ccgaagcaat cgcaaccgtt gggcgtgagt ggcagacaat tgttcagaca agtttcgctt
     1741 tgcgcggttt gtagcgccgt acacgactgc ccctgaatgg aagccgccgc gtcgtatgcg
     1801 cagcccggat ccccactcgg agggtagccc ggcgcaaccg acaacggatc gcagcgctgg
     1861 ctccactcgc agcggtcgcc ccccgcgcct gcattccgat cgaagtagca atctgagcgg
     1921 cattggccgt tcgtttcggt gtgagggccg ctcgagattg actgctcggt gtcgtcgcac
     1981 gggcccaagc actcggggtc cagcgcgtcg atcaacccgt cttcatcgtc gtcttcgcag
     2041 ttgccacaag cgtaggattt gccagaacag gtcgtgggag tacatacgaa gggggcctca
     2101 cctccggtcg ccgacggcgc gccagaagtg gctcctcccg cactcgtgct cccgcccgca
     2161 ccggcgcggg cccagtcgcg cccgacttcg gtttcactcg tgcactgcag cgcgactccc
     2221 agaacacacg agaacgccag ccacgaccac ctgctcgcga gcgcggagct cgagcgggtt
     2281 cgttggctcg cgacgcgggc gggcatcgcc tggttattga ccgagtccgg tctcgacgcc
     2341 acggcgatgc gccgaacccg ggtaagtctt cgagaatcgc tcggcgcgtg cgctggcctc
     2401 agcttgtcgg cctagtcgtc ggagcgcttc gatcgcgatg acttcacgct cttgccggag
     2461 tagccccgaa gcaaagcgga cctcgtgggt gcgcgtgagg gctaacgctg cggtgggatc
     2521 ctgcgcgagc gctttgcggg ctcgttccag cagttcggct tcggacgccg cactcggcgt
     2581 gttgggcgca acgcgcggcg gttgcgctcg cttgggctcc gcttcctgag caggagcggg
     2641 ctccgcggac gagggggtgg gcgagggcgg aaccaagctc gagtccaccg ccgggggcga
     2701 aggcggaagc ggcgcgggct ggcttggaac agtgatggtc ctcgaaggtg ccggaaggac
     2761 gggcggggtc gggtctggcg cctttcgatt cacgagcacg accacggcaa cgcccgcggc
     2821 caccacgcca accgccagcc ccacgagtcc cagtttcccg gctcgcgaga gcggggcccg
     2881 tgtcgcgtgg aggggcggct cgggctgggg ttcgagggct tgcggtcctg cgacaccact
     2941 ccccatcaaa tcccgaagcc gcgactccag cgccgccaat tgagagtcat tcggcacgtc
     3001 tcgctcggct tcttggaaca gccgctgcag accttcggag ctgggggtat gcggtgcaag
     3061 cacccagcgg gggggatctc gaaactcgct catggggccg ccttcgagcc gcgctcttgc
     3121 gccaaggtaa gtagcttttc gcgcgcggcg tacagtcgcg aataagcggt ctgctgcggg
     3181 cagcccaaga tttcggccac ctctttcatc gtgaaaccct cgacctcgta cagcactaga
     3241 acctcgcgct tgtcgtcgtc gagctgcgaa agcaaagacg tgagcaggtg ccgagcctcc
     3301 aagttgcctt gcggctcgct tcccagagtc gggctctcgg cgaacggctc agcgtaaggc
     3361 cgctcccgaa cgacatgggc tcgtcgccgg aagtcggacg cgacccgcaa gcagatcccg
     3421 tatagccaag tccgcacgct ggagcggcct tcgaaactgg ggagctttct gaacgcggtc
     3481 acgaatactt cctggcacac atcctccaag tcggcctcgc gaacacccag atagcgaagc
     3541 gagcgccaga tgtagcgggc gttcgctttg aaaacgctat cgaagtcggg cgtttccggg
     3601 ctggagctca tgccgagaac tccatttgcg gtcgcgcacg agtgaggatc gagggcgcgg
     3661 aaatctgaag gtcgattgga gggtgattga ccatgagtgg cgcggcgccg gttcgttttc
     3721 tgatcacggg gaccacctca atcccatttt cacggccaag aacgcccagg cttgctcgaa
     3781 cacgctatcg gtagttcccg tctcgtttct ccaaatgaac ttttcacgtt gtaagggcag
     3841 ttcgactcgt gccgcgagcg caaatttcag ccgctctgat gcgcgcaggg tgccttctac
     3901 gctgcccccg accgcatcga gcacgcgggc gcgcgagaca ttctgatcga agccgtaccc
     3961 cgcggcgctc agcctcccca ggtgctgtcc tgcgcaggtg cggagggccc acctctctcc
     4021 cgtgagcacg tttccacaaa cctccaggcc gacccgggcc aagaagaagc gggctccggc
     4081 gtcccgatct acgacactcg cgctctcgct gcggtaagca tcgagggaca agcgaacttc
     4141 cggcgctcgc ggcgcgctgg cactgaatgc cagctcgccc cctggcgcaa ggccgggcaa
     4201 agcgcccgcg gtaagcaaaa ggtgcgcgcc gatctcgaat tgccacggct cccgtcgcgc
     4261 gggcgtatcc gccggcaacg tgagcggcgt gggttcccca gtcgttgggc gggatggcgc
     4321 ggcgcgcgcg cggtcggcac tctggcgcgg gtcagctgcc gttttgccgc cgctgatact
     4381 cggggcttcc ggtcccttcg gtggcttcgc ctccggaact ggctcactcg ggggcgcggt
     4441 gggcgtatcc acgagtaggg ccacgaccaa agccagtgaa tcgtccagag acgagcagtg
     4501 tttgccccgg ctcctcagct cacgctggcc gagaatgccc atcgggtcgg acagcgtgag
     4561 cttggcggac caagttcccc cgatgcgcgc cagcttcagc gccaaccgaa gctcgcccgg
     4621 tcgatcggtg aacacgcgcc gaccaagccg cgcctcgacc tgcttctcca gatcaggtgc
     4681 cgagatgcag cccgtcgagt ccgatgcatc ctccagctgc aggtgaacgc ggggagctcc
     4741 gagggcctgg gagggcagtg cgatgccgag caacgcacct gtcatggcga gaagcgttac
     4801 cgcccgtaat ctgcggctcg ggtgggctcg aaaccttttc gaggagggcg agtaagaaat
     4861 cccaccaggt ctcgccactg accgttcaat gcactccaag ttgactctgc atacctggca
     4921 acgcccactt tgggcactcc ttttgctgag cccgctcggg ctcggtgcgt tgggctgctc
     4981 tggacgcaca caatctgacc ctggcgacat gcctgcgggc aacagcggtc tgcccggcgc
     5041 gggggcgaac tcgggaggtg cggccggtgc cggcgcccgc ccaggaacga gcggaggcaa
     5101 cggcagcgga gcgagtgcag gcagcggagc gagcgcgggc agcggagcga gcgcgggcga
     5161 ctcgaccgga gctggtaccg gttccacagg cggcgtcgcc accacgcagt gcaataacgg
     5221 tctggacgac gacggggacg ggctcgccga tgggttcgac gccgaatgca ccggtgcact
     5281 ggacaacgac gagggctctt ttgcaacagg aatccccgga gataatcgcg atcccaaatg
     5341 gcaggattgc ttcttcgatg gcaactccgg cgcgggcgac gatcactgcc gctacagcac
     5401 aggctgcctg accggcgacc ttccaagtga ctcgtcgcag tgtcaactcg cacagagttg
     5461 ccttggtttt tgcgcaccgc ttacgccgaa cggctgcgat tgcttcgggt gctgcacggt
     5521 cgtgctcgga agcggcaaag agattgacgt ccagattgga tcgagctgct cgctcgacaa
     5581 ggtcatggac gcagccgctt gcccagtgtg ccagaagagc acgcagtgcc aaaacgattg
     5641 cggcaagtgc gatctttgcg cgggcaaaac ggcagcggat ctggatccga gttgcacgcc
     5701 ggagacgccg cctccacccc cgggaacgga gccgccgccg cccagcaccg agcctccgcc
     5761 gccatcctat acctgcgagc cgggccaggc gaagtgtggg ccgggcttac ccgcctgcag
     5821 tggttcggag tattgcgcgc agggctgctg catcctgagc atccgataac aaccgatgag
     5881 ccgtgatccc tgccggcggg gtagggatca cagccgggcc gagatcatcg cgaggcggag
     5941 atcaccgcga ggcgggagat catagcttgc tggccacgag ctcgcgcagc gtggagagat
     6001 cgaagggctt ctccaggaag ggcaggttca cgcggtccaa gaactcgcgc gcgtttgccg
     6061 tgaaggctcc gccggtcatg aagatgattc gattctcgat gctcaggttg gctcgcaaga
     6121 cggcttcgta gaattcggcg ccggtcattt ggggcatcat cacgtcgcag agtacgacgt
     6181 cgaattgttc gcctcgctgc agcatgtcga gggcgtgctc ggcgctattg accacgacca
     6241 cgtcatagtc gttgagcagg gcacggcgaa ccaacgcacc gacggcctgg tcgtcgtcga
     6301 tcacgagtac gcgcgatcga cgggccgggt tagagaacga tgtgcgggag ttgctgccgt
     6361 gtgagcgcgg gctcacttca ccctccgccg agggcaaggc gactacgaag cgcgtgcctt
     6421 tcccgagctg actttcgaag ctgatgtctc cccccaggga actcacgatg cggtgacaaa
     6481 tcgctagccc gagtcccgtg cccgagcccg gcggtttggt gctgtaaaag ggggtgaata
     6541 ggcggcgttg aacttcggga ggcatgccgc tacccgtatc ggcgatggag atcacgatac
     6601 gcccctggtc gtcgagctcg gtgctgactc tcacctcgtt gcga
//
LOCUS       DASTKU010000272         3064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_499354, whole genome shotgun sequence.
ACCESSION   DASTKU010000272 DASTKU010000000
VERSION     DASTKU010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499354"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..975)
                     /locus_tag="VFQ61_09810"
     CDS             complement(337..975)
                     /locus_tag="VFQ61_09810"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HET9954790.1"
                     /translation="MASTHFVPRARALLAQLEQADQSYGLFGARAHRYRLNPCVEEAA
                     LARRERELGVRLPEAYRQFLTELGNGGAGPSYGVFAFEGGESEDYTKYERLAHPFAYT
                     EEYNPTWLLDEDEDEGTEEEEDSSEDEDDPRHRYWEAFDDRGALYICHHGCGGRSFLV
                     VSGRAAGQVWCDGVADDGGYSPELDASGAPFTFDTWYLDWLERALASLAGPR"
     gene            1466..2041
                     /locus_tag="VFQ61_09815"
     CDS             1466..2041
                     /locus_tag="VFQ61_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ricin-type beta-trefoil lectin domain protein"
                     /protein_id="HET9954791.1"
                     /translation="MLALVAGCAAQDGTEDGRDGSSELAASKQSLSNPIQLVNFVSQD
                     QCLAVAAGLNKDGQAIVSWGCENSASQTWSFEGDQLRSNVGENKCLGVNEVARPKRFG
                     GYTDGARGVLRTCSAYAPEYVGWKKLAMPQYSIGDGSECFRLAPLFDQQKVLGVLGGV
                     VTSGKASGVWSDFQGLSHLDQVWCSRPAVPY"
     gene            2134..>3064
                     /locus_tag="VFQ61_09820"
     CDS             2134..>3064
                     /locus_tag="VFQ61_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9954792.1"
                     /translation="MRILFDESLVSRVSWVGLLVLSACEVSVDAPESTEPSAAASQQR
                     LANTSRGNHGTNGDYDGDGRTDYAVFQPRNSGNWRVRSSGVSAAVTEVQWGLPNDISV
                     PADYDGDGLTDIAVFRPGEGNWYTRFSSLRYVLSPTDYYNLTPVQWGLPGDTPVPGDY
                     NGDGFVDRAVFRPSDRNWYLNFSAQGLFFPTQGLGPQPAVHFGEPNDVPVPGDYDGNG
                     TTDLAVFRPGSPARWILRDPATGGELPGVEWESGVIPVPGDYDNDGATDMAVFDPRSG
                     NWRVRAPNGVVTQTHWGEFLDVPVPGDYDGDSQL"
BASE COUNT          591 a          885 c          973 g          615 t
ORIGIN      
        1 cgcgcggagc tcgtttcctc cgacaaccaa cgcaggcctg gaaccgcgaa atcaatctga
       61 catgcgcaag tcacctggcg ttgcgacatt gctgtcggag cgcgcgacgt tcacgcgacg
      121 aaaccgtcga gctgaacgga acttcaagga gatctgcgcc gcgccacccg ccacgctgtg
      181 cacatgtgaa tcaaacatcg cgggtcgcgt ggcgcccaag cagcgcgtcg cacggacgaa
      241 acgacttttt cgtcgtgctc ggtcagtcgg cggtcggtag ccgcgcgtct tggctcgggc
      301 tacgtcgatg tcgcgctctt gctcttgctc gccacttcag cgtgggcccg cgagcgaggc
      361 aagagctctc tcgagccaat cgaggtacca ggtgtcgaag gtgaacggcg caccggaagc
      421 gtccaactcc gggctgtagc caccgtcgtc cgcgacgccg tcgcaccaga cttgacccgc
      481 agcacggccc gaaaccacga ggaaggatcg ccccccacag ccgtggtgac agatgtatag
      541 cgcgcctcgg tcgtcgaagg cttcccaata gcgatgccgg ggatcgtcct cgtcctcgct
      601 agagtcttcc tcctcctcag tgccctcgtc ttcgtcttca tccagcagcc aggtcgggtt
      661 gtattcttcg gtgtacgcga agggatgagc gagccgctca tacttcgtgt agtcctcgct
      721 ttcgccccct tcgaaggcaa acactccgta gctcgggcct gctccgccgt tgccgagctc
      781 ggtgaggaat tgacgatacg cctcgggcag gcgcacaccg agctctcgct cgcggcgcgc
      841 caatgccgcc tcctccacgc acgggttcag tcggtatcga tgtgcccgcg cgccgaacaa
      901 tccatagctt tggtccgctt gctcaagctg cgccaagaga gcgcgcgctc gcgggacgaa
      961 gtgcgtggaa gccatggtgc ggatcatatc gcggagtgag cctcagctgg cgggggcgtg
     1021 ccgtggagct tccggcacga agagcgctcc ctgggcgaag agcctattcc gtgaatcgag
     1081 aatttgagca cgagagtaga tgtggggtcc ggttccgctc ggaagcgagg ccgaaaacat
     1141 gtccggctcc aacgcacacg cgcgattgaa gccgtgcgat cgccgaacgg tgggaacttt
     1201 ccgagaacgt ccgtggacgt tctcggacgc atccgagcac cgagcagtaa ccgccgcgca
     1261 aaagctctgg aattggtcga gaagggcggc aagttgattg cactcgtacg gcgaagccac
     1321 tggcatagtc ctcgcacgct catctacgag catattctga tcgacgaggc gcaggacttg
     1381 agttccgtcg agctcgcggt ccgcgttcgt ggatcggaaa ggataaatga tgagcactac
     1441 taaactgtgc gggttcgccg gtctcatgct tgcgctcgtc gctggctgtg cggcgcaaga
     1501 tggtactgaa gacggccgcg atggcagctc agagctcgct gcttccaagc aatcactcag
     1561 taatcctatt caattagtca acttcgtatc gcaggaccaa tgtctagcgg ttgcggcagg
     1621 gctcaacaaa gacggtcagg ccatcgtatc ttgggggtgt gaaaacagcg cgagtcagac
     1681 gtggagtttc gaaggcgatc agctgaggag caatgtgggg gagaacaagt gccttggtgt
     1741 gaatgaagtg gctcgtccga agcggtttgg cggctatacc gatggcgcgc gtggggtgct
     1801 tcgtacttgt tcggcgtatg ctcctgaata tgtcgggtgg aagaagttag cgatgccgca
     1861 gtacagtatc ggtgatggaa gcgaatgctt ccgcttggct ccactgttcg atcaacagaa
     1921 agttttgggt gtgctgggcg gcgtggtgac gagcgggaag gcctcgggcg tctggagcga
     1981 ctttcagggc ctttcacatt tggatcaagt ctggtgctcg cgtcccgcag tgccgtattg
     2041 attttcctcg tgctcgaagc acggcggcac gcgcagtctg cggacgcaag gcaggctcac
     2101 ttgggggcct caatctagtt tggagaatga acaatgcgaa tactgtttga tgaatcgctc
     2161 gtgtcacggg tttcttgggt gggattgttg gtcttgtcgg cttgcgaggt cagcgtcgac
     2221 gcaccggaat ctactgagcc gagcgcagcg gcgagccagc aaagactagc caatacctca
     2281 cggggcaatc atggaaccaa cggcgactac gatggagatg gacgcactga ttacgcagta
     2341 tttcaaccca ggaactcggg caattggcgc gtccgttcga gcggcgttag cgccgcagtc
     2401 actgaagttc agtgggggtt gccgaacgac atctcggtcc cagctgacta cgacggcgat
     2461 ggtctcacgg atattgccgt gtttcgacca ggggaaggca attggtatac acgcttcagt
     2521 agcttgcgtt acgtgctaag ccccacggat tactacaacc tgactcccgt ccagtggggt
     2581 ttgcccgggg acacccccgt tccaggcgac tacaacggcg acggtttcgt cgatcgcgcg
     2641 gtgtttcgac cgagcgatcg gaattggtac ctcaacttca gcgctcaagg gctgttcttc
     2701 ccaacgcagg ggttgggtcc tcaacccgcc gtgcattttg gcgagcccaa cgacgtgccg
     2761 gtgcccggcg attacgacgg caacggcacc actgacctag cggtattcag acccggctct
     2821 ccggcgcgat ggatcttgcg cgacccggcg acgggcggag aactgcccgg cgtggaatgg
     2881 gaatcaggcg tcatcccggt gcccggtgac tatgataatg atggcgcgac cgatatggcc
     2941 gtattcgacc cgcgttccgg caactggcgc gtgcgtgcgc cgaatggcgt tgttactcag
     3001 acgcattggg gtgagtttct cgacgtgccc gtgccgggcg actacgacgg cgactcacag
     3061 ctgg
//
LOCUS       DASTKU010000273         8460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_721056, whole genome shotgun sequence.
ACCESSION   DASTKU010000273 DASTKU010000000
VERSION     DASTKU010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8460
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721056"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..670)
                     /locus_tag="VFQ61_09825"
     CDS             complement(38..670)
                     /locus_tag="VFQ61_09825"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9954793.1"
                     /translation="MKSVAPLQSVSPFPTDRLGDAELVQAVLQGNSEAAGLVWDRYSP
                     LVRSVLRGNLGLDADVEDLLQEVFMVFLRSASELRSQSALRSFLVAVAVRLVLVELRR
                     RRVRRWITLSPDGELPEAAARPEDVEGAAALRGLYRLLERMPHRPRVAFVLRHVEGLE
                     LNEVARALNVSASTVKREIGRARKAIFTRAKRSEPALWQFLSRFEEDADA"
     gene            complement(791..1105)
                     /locus_tag="VFQ61_09830"
     CDS             complement(791..1105)
                     /locus_tag="VFQ61_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954794.1"
                     /translation="MTGTLSGAVQGIVGAATQTSDGLEIQVEDHLVTDEGCVLQTADV
                     VKLVPVPGLDNAFHQSTRFDIVSGTGKFVRANGSFMSHGEADLVRGTIVSRYEGRICG
                     IE"
     gene            complement(1102..1281)
                     /locus_tag="VFQ61_09835"
     CDS             complement(1102..1281)
                     /locus_tag="VFQ61_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954795.1"
                     /translation="MNYKSFLALPALSLIVLASYSPAVAHAPPDRTSKSCISIGGTIS
                     AAMVGPAGSQAVVGS"
     gene            complement(1434..2783)
                     /locus_tag="VFQ61_09840"
     CDS             complement(1434..2783)
                     /locus_tag="VFQ61_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954796.1"
                     /translation="MAHDLLADHGGPGGSGEEYVPPPVEPAPPQQGQYWKVASGFEHE
                     VWLVDQSNSPNKSFGGTLYVYDDEALRQNASAAVPQVVDLGGATTNLCLSQTGAAPVR
                     PHMLVFNHAQTHAVLAFVASGHVVIFDAATRSPVRCFRTEPGAGGARQAHAVWPAPDD
                     SYILVANQNGKKFERISTDFPSGTFVQEPGATLDLANCTTPNGFPCQDPALRPDNAPI
                     CPFVPENGFPAYVSLRGGGMLVVNPLTTPMSILAEYDADHIPRDGCGFIEAKGWVYGN
                     GGGGNPANPDGWFLYRLPVAGPYSALNPPNQPSPYVVAHDDRAPRDAHGVAKSKQDKY
                     VYFFDRAANVAEMYDAASAAYVKTENLVSPWSADPTVDLAGEAPDGKYVYASTRGPNP
                     LSGDPHASTGSTPGLLVIELLQDGRDLAVRGLARIHNVDAGGVERADAHAIRVRRVH"
     gene            3208..3399
                     /locus_tag="VFQ61_09845"
     CDS             3208..3399
                     /locus_tag="VFQ61_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954797.1"
                     /translation="MCLICIDFDRGALKPKEARRALREMRSQLDPDHAREVEQKLDLA
                     GEQAARDPAASSGPPASRP"
     gene            3851..5752
                     /locus_tag="VFQ61_09850"
     CDS             3851..5752
                     /locus_tag="VFQ61_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9954798.1"
                     /translation="MNEQIAAAPSGWMKARTGTLRWLATFRSAWRYGARTLRLVAQSS
                     RALLAAQVACTALVAVLPLGVAYVGKHIIDAVVAGQARATLRWVAAELALVLAVSAAQ
                     RSLGLVRQLLSAKLGADINLAILEKAQHLALPFFENTEFYDKLTRARREASSRPVSLL
                     TDCFALLASLVTLLGYGVLLTGFSAWVLPVLVVATVPSTLSEMHYSKLVFRVRNWRSP
                     DTRRLLYLEHVLANDEHAKEVKLFGLGPLLLGRYRTLAAEFYREDRKLALRRVVLTQS
                     LGVLGVAAFYATYAVMAWAAVQRQISLGELTLYVVSLRQGQQTFTSALSGIGNIYEHN
                     LYMSNLFEYLDMPAATLDAATPSPAIDTLEAREPSSRNAAEGLVSLPGERGVRFENVG
                     FRYPNSSTFALRGLDLYIAPGESLALVGENGAGKTTFVKLLTRLYEPSEGRILLDGKP
                     LRSYPIEDLRRRFAVIFQDFNQYQLRVRENVGFGSVHHLEDTARVQRAVERGGAAELV
                     EQLPKGLEQPLGHWFEKGSELSGGQWQRLALARAFMREEADMLILDEPTAALDASAEH
                     AVFQRFRELSRGRTSLIISHRFPTVRMADRILVLEHGKIVEAGPHEELLRLNGRYARW
                     FALQAEGYR"
     gene            complement(5792..6055)
                     /locus_tag="VFQ61_09855"
     CDS             complement(5792..6055)
                     /locus_tag="VFQ61_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HET9954799.1"
                     /translation="MALSDDFQAAQVTVKQLKRSPNTDELLQLYSLYKQASEGDVRGE
                     RPGMLDFKARAKYDAWTSRKGMTQEAAMSAYVALVSSLVKQYS"
     gene            6837..>8460
                     /locus_tag="VFQ61_09860"
     CDS             6837..>8460
                     /locus_tag="VFQ61_09860"
                     /inference="COORDINATES: protein motif:HMM:NF025174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET9954800.1"
                     /translation="MTTNQTAARQQVRSPARDSDRPLRSDSSPPESGPVPIRRSITPP
                     ADNSQTYRYVNVNEFQPNVPGWQEFGTVTQWDRVANNALRLTNAAGYAVQVSFLSTRA
                     LRVQFRPTPNAQYQLDGGSYAVVNRALAPVNLTIQEIDRGGATLYVQTGELDVYIGLA
                     PYGMAVYRNGQLITQDMYGTNLVFSNSAVACLRSAPGSENYYGLGEKAGAQLNKKYFT
                     LTCFNYDNFTYQGSAEDGIIPQGNAGGPLNPSEPLYNSMPFLLAVNTQQDSSGQPAYA
                     YGLFLDNVAQSYFNLGSNDYSDMSGKYYLGALFGDLDYYILVGEGSGSQAIRSVVSQY
                     TALTGRPAMPPKYALGYHQGCYGYYDVSILLGAAQAYRLSQIPIDGLHIDVDFQNNYR
                     TFTISPRKFPAPQAMFEILHQMGFKCSANITGIISANPLDEDGNTNTPYPARDAWVTV
                     SPSSQITVKPGVIPPFIYDTRQGEAESPNLFIANEGYGDEDTSLYPFGYNPYPYPTPL
                     YPQGQESLGTYGFYSDMGRPDVQQWWGQQYAYL"
BASE COUNT         1620 a         2674 c         2578 g         1588 t
ORIGIN      
        1 gcgcgactgc gccagcgccc ccagcacttc gaatggatca cgcatcggcg tcctcctcga
       61 agcggctcag gaattgccaa agcgctggct ccgagcgttt ggcccgcgtg aagatcgcct
      121 tacgagcgcg gccgatttcc cgcttcacgg tcgaagcgga gacgttcaga gcacgagcca
      181 cttcgttcaa ttcgagcccc tctacgtgcc gaagcacgaa ggcgacccgt ggccgatgcg
      241 gcattcgctc cagcaatcta tacagaccac gcagcgcggc cgcgccctcc acgtcttcgg
      301 gccgagcggc agcttcaggc aactcgccat cgggagacag tgtaatccag cgtcgcaccc
      361 gccgccggcg gagttcgacc aataccagac gcaccgccac cgcgacgagg aacgagcgta
      421 gtgcgctctg cgaccgaagc tcggaggcgc tccgcaagaa gaccatgaag acctcctgca
      481 gcaggtcttc tacatccgca tcgagcccta ggtttccgcg aagtacgctg cgaactagcg
      541 gcgagtagcg gtcccaaacc aggcccgccg cctccgagtt accctgaagc accgcttgca
      601 ccagctcggc gtcgcccaaa cgatcggttg ggaagggcga gacggactgc aggggggcaa
      661 cggacttcaa cactcgtcca gaagtgaccc tgaggggcga ggccgggtca ccaaaatgca
      721 ggctcggacg attctcagtt ctagaaaagt ctacttgggt tggagccgtg cctctcttgc
      781 ggcaagccgg tcattcgatc ccgcaaatac ggccttcgta gcgactcacg atcgttccgc
      841 gcaccaagtc cgcctctccg tggctcatga agctgccgtt cgcgcgtacg aacttgcctg
      901 tcccagaaac gatgtcgaag cgggtgctct gatgaaaggc gttgtccagc cccggtactg
      961 gcaccagctt cacgacgtct gccgtctgca acacgcaccc ctcgtcagtt acgaggtgat
     1021 cttcgacctg gatttcgagc ccgtcactgg tttgagtggc ggcgccgacg atgccctgca
     1081 cggcgccact caacgtgccg gtcacgatcc cacgacggcc tgtgaacccg cgggcccgac
     1141 cattgcagcc gaaatcgtgc ccccaatcga gatgcagctc ttgctggtgc gatccggcgg
     1201 tgcgtgggcg actgccggtg aatacgacgc gagcacgatc aacgacagag ccggtagagc
     1261 caagaagctt ttgtagttca tgatgcgttt tgcacgaatg gtgagagggc cagcccgcac
     1321 ccgcgcgagc tggcccaaga caccatgtct tcaccccgcc gaattcgtca tccgaatcgc
     1381 ggccgacttt tcggagcgat tctcatcgaa tggacggtct ctcgagcgat tcctcagtga
     1441 actcggcgca cgcgaatcgc gtgcgcgtcg gctcgttcga cgccaccggc gtcgacattg
     1501 tgaatgcgag ctagccctcg caccgccaga tctcggccgt cttgcagtaa ctcgatcacc
     1561 agcaatcctg gagtcgatcc ggtcgaagcg tggggatctc ccgagagagg attgggccct
     1621 cgcgtggacg catagacgta cttgccgtcc ggggcctcac cggccagatc gacggtcgga
     1681 tcggctgacc acggggagac cagattctcc gtcttcacgt atgcagccga ggctgcatcg
     1741 tacatctccg cgacgttggc cgcccgatcg aagaaataaa cgtacttgtc ctgcttcgac
     1801 ttcgcgacac catgtgcgtc gcgcggagcc cgatcgtcgt gcgcaacgac gtaggggctc
     1861 ggctgattgg gcgggttgag cgcactgtaa ggccctgcca ccggcagacg atacaagaac
     1921 caaccgtctg ggttggcggg gttgccaccg ccaccattgc cgtaaaccca gcctttggcc
     1981 tcgatgaaac cgcagccatc ccgtggaatg tgatcggcat cgtattccgc gaggatgctc
     2041 atcggagtcg tcagcggatt gaccaccaac attcctccgc cgcgcaggga tacgtacgct
     2101 ggaaagccat tctccggcac gaacgggcaa atcggcgcgt tgtcggggcg cagcgccggg
     2161 tcttggcagg ggaacccgtt cggcgtcgtg cagttcgcca aatccaaggt cgcgcccggc
     2221 tcttgaacaa aagttcccga cggaaaatcg gtagaaatgc gctcgaactt cttgccgttt
     2281 tgattggcga ccagaatgta gctgtcatct ggtgccggcc aaactgcatg cgcctgtctc
     2341 gcgccgcccg ccccaggctc ggtgcggaag caacgcaccg ggctgcgcgt tgcggcgtcg
     2401 aagatcacga catgccccga ggccacgaat gccaatacgg cgtgcgtttg tgcgtgattg
     2461 aagacgagca tgtgaggccg caccggcgcc gcccccgtct gactcaaaca caaattggtc
     2521 gtagcaccgc ccaaatccac gacctgggga acggcggcac tcgcgttctg gcgcaatgct
     2581 tcgtcatcgt agacatagag agtgcctcca aagctcttgt tcgggctatt cgattgatcc
     2641 accagccaga cctcgtgttc gaagcctgat gccaccttcc agtattgccc ttgttgcggc
     2701 ggcgcaggct ctacgggtgg tggaacgtac tcttctcccg agccccctgg cccgccgtga
     2761 tcggccaaca aatcgtgagc caaggtcgtg gcgacagcga gactggcaag ggccgcagcc
     2821 cgatacctcg accgcgctcc cggaatcgat agctttcccg atttcatata accctccgag
     2881 ttggagcgcg aagcctaaca ctcaagattc aacgttgtca cctgcgctcg cgtgcgtgcg
     2941 gtcagatccg atccgctcgc gtgtgagcga cctagcctct cgagttagac gctggccaga
     3001 gttcgctggc cagagttcgc tggcccagag gttcccggca aagctttgcg cggtgagcgc
     3061 gctatcgagc acgcgtcgcc acgactcctc gacgtccagc cgacttacac cgacctcgaa
     3121 gctgcggccc cctggcaatc tctcgcactt cgtcgcctac cgaactggat ggtggacaaa
     3181 tgcccgcgcc tccataaggt tacgtgcatg tgcctgatct gcatcgactt cgaccgcgga
     3241 gctctgaaac ccaaagaggc gcggcgcgcg ttgcgcgaaa tgcgctcgca actcgatccc
     3301 gatcacgctc gcgaggtcga acaaaagctc gacctcgcgg gagagcaggc cgcgcgggat
     3361 cccgcggcct cgtccggtcc gcctgcttct cgcccgtagc gttcgtgtgc gtgatgcagg
     3421 cgaatcgagc cctagccggg ctgccgaatg cccgagcgcc gacggcacga gcgggatcct
     3481 cgagcggggc agctgggcac aacgagatgg gcgcgattgg agggccggat aaggtgctcc
     3541 attggtggcc ggatcgcagg gcgcgataga gcgcagaacc gcaacgggca cggggggtag
     3601 ttggccaacg aacaacgggc gcgaggcgcg tcccaagggc acggccgcgc agcctcatga
     3661 cccttagcac gcttatccac cgcgccggcc ccctcaatat cgggtacgcg catgcgtcac
     3721 gcgcggattg cgtcgccgtc tcatcccttc gagagcggcg ttcgagccct cgtacgcagc
     3781 accgacgggc tgattgacgg cgttggcact agctgcgtct tggaatcgtc ttaagttcga
     3841 actggtcgag atgaacgaac aaatcgcggc ggcgccaagt ggatggatga aagcccgcac
     3901 gggaacgctg cgctggctcg ccacgtttcg ctccgcttgg cggtacggcg cacgcacctt
     3961 gcgcttggtc gcccagtctt cgcgcgcctt gctggcggcc caagtggctt gcacggcctt
     4021 ggtggcggtg ttgccgctcg gagtggcgta cgtgggcaag cacatcatcg acgccgtcgt
     4081 cgcggggcag gcgcgcgcca cgctgcgctg ggtcgcggcc gagcttgccc tggttttggc
     4141 cgtcagcgcc gcccagcgca gcctcggcct cgtgcggcag ctgctcagcg ccaaactcgg
     4201 cgccgatatc aacctggcga tcttggagaa ggcgcagcac ttggcgctgc cgttcttcga
     4261 gaacaccgag ttttacgaca agctgacgcg ggccaggcga gaagcgtcct cgcggccagt
     4321 gtcgctcttg accgactgtt tcgcgctgct cgcgagtttg gtcactctgc ttggctatgg
     4381 cgtgttgctg accggatttt ccgcgtgggt gctgccggtc ctggtggtgg ccacggtgcc
     4441 ctcgacactc tccgagatgc actactcgaa gctcgtcttc cgcgtgcgca actggcgatc
     4501 tccggatacg cgccggctcc tctatctcga gcacgtgttg gccaacgacg agcacgccaa
     4561 agaggtcaag ttgtttgggc tcgggccgct gctgctcgga cgctatcgga ctctagcggc
     4621 cgagttctat cgcgaagatc gtaagctcgc cttacggcgc gtggtgctca cgcagtcact
     4681 cggtgtcctc ggcgtggccg cgttctacgc cacttacgcg gtgatggcgt gggccgcagt
     4741 ccagcgccaa atcagcctcg gcgagctcac gctctacgtc gtttcgctcc ggcagggaca
     4801 acagactttc acctcggcct tgtccggtat cggcaacatc tacgagcaca acttgtatat
     4861 gtcgaatttg ttcgagtacc tcgatatgcc tgccgccacg ctcgacgcgg ccaccccgag
     4921 tccggcaatc gatactctcg aggctcgcga accgagctct cgaaacgcgg ccgaaggttt
     4981 agtctcactc cctggcgagc gtggcgtgcg cttcgaaaat gtgggcttcc gttaccccaa
     5041 ctcgagcacg ttcgcgctgc gcgggctcga cttgtacatt gcgccgggtg agagcctagc
     5101 gctggtgggt gagaacggtg cgggcaagac cacgttcgtc aagttactga cacgcctcta
     5161 cgagccgagc gaaggtcgca ttctactcga tggtaagccg ctccgcagct acccgatcga
     5221 ggacctgcgc cggcgcttcg cggtgatctt tcaggacttc aaccaatacc agctccgagt
     5281 gcgagagaac gtgggctttg gcagcgttca ccacctagaa gacacggcgc gggtgcaacg
     5341 cgcggtcgaa cgcggaggag cggcggagct cgtggagcag cttccgaagg gactcgagca
     5401 accattggga cactggttcg aaaagggatc cgagctctcc gggggacagt ggcagaggct
     5461 cgcgcttgcg cgggcgttca tgcgcgaaga agcggacatg ctgatcttgg acgaacccac
     5521 ggccgcgttg gacgcgagcg cggagcacgc cgtgttccag cgctttcgtg agctatcacg
     5581 cggccggacg agcttgatca tctctcaccg tttcccaacg gttcgcatgg cggatcgcat
     5641 tctcgtcctg gagcacggga agattgtcga agccgggcct cacgaagaac tccttcgttt
     5701 gaatggccgc tacgcccgct ggtttgcgct ccaagccgag ggctaccgct gaacgacgcg
     5761 gcagcctcca gcacgccctg cggtgaggcg atcaagaata ctgtttgacc aacgaactca
     5821 cgagcgcgac atacgcgctc atcgcggctt cctgcgtcat gcccttacga ctcgtccaag
     5881 cgtcgtactt ggctcgcgcc ttgaaatcga gcatccccgg tcgctctccg cgcacatccc
     5941 cctcgctggc ctgcttgtag agcgaatata gctgcagcaa ctcatccgta ttcggagaac
     6001 gcttgagctg cttgaccgtg acctgggccg cctgaaaatc gtcagaaaga gccatgcgcg
     6061 ccactatagc cgatcgccga ccccccgttg agcccgatga ccatgcgagt cccccgcgag
     6121 ccggcccaca ctccgagctg gcgcgcacgt tgcgcgcgcg atcgggcctg agcgagcagc
     6181 acggtatggc atggattcgg ctgacctgtg ctagtggcgc ggtcccatag gtaaacgatg
     6241 gtttgcgtag cacgaagatg gtgaggacca tccgtcgtga gaacaatatg ttttgtggtg
     6301 aatagagacg ggcgccctcg ttcggccgaa ccggacaacc tcgccgagat ggatgcgaac
     6361 atcagacgct cgatcgatgt ttcgattcgg agtagcccta cacagactct cgcgggcgag
     6421 aactagactg acctcggctt gccgcgggga aggtgaactc cgccgatttg caagcaggga
     6481 tccgggtaag tgagggcgcc gatcgggtac atggcggtat tgcttgcgag agcgccggtc
     6541 gcccgatgtg gctgccaagg actcaacttc gatgtgcgcg gagcatcggt tggcgtccga
     6601 atcggacatg ggttggctaa gttcgtttgc ggatgcgacg gagtccgact ctgagttgtg
     6661 agtcggccag tacgtcgcaa gtcgaatgtt cgacagagtc gtgcatgccc gcgcaaacga
     6721 ccaggcggca cattcgttgc tcaaggcggg gcccgccgcc gcccgcccaa tgtgattggg
     6781 tgggacggcg gacccttacc cacacggcac gcccacaccg cggagcaatg aacacgatga
     6841 ccacgaatca gacggcagcg cgacagcaag tgcgttcccc agcacgcgat tccgatcgcc
     6901 cccttcggtc ggattcgtcg ccgcccgaga gcggtccggt tccgattagg cggagcatta
     6961 ccccaccggc agacaactcg cagacctatc gatacgtgaa tgtgaacgag ttccagccca
     7021 acgtgcccgg ctggcaagag tttggaacgg taacgcaatg ggatcgggtc gccaacaatg
     7081 ccttgcgatt gacgaacgcg gccggatacg cggtgcaggt gagctttctc tcgacccgtg
     7141 cgttgcgggt gcagttccgg ccgactccaa acgcgcagta tcaactcgac ggggggtcgt
     7201 atgcggtcgt gaatcgagcg ctggcgccag tcaacttgac gattcaagag atcgatcgcg
     7261 gcggggcgac tctgtatgta caaaccggtg aattggacgt ttacattggc ctcgcgccgt
     7321 acggaatggc cgtgtatcgc aacggtcagt tgatcacgca ggatatgtac ggcaccaatc
     7381 tggtgttcag taatagcgcc gtggcttgcc tgcgcagcgc gcccggcagc gagaactact
     7441 acgggctcgg tgagaaagcg ggcgctcagc tgaacaagaa gtacttcacc ctgacctgct
     7501 tcaactacga taatttcacc tatcagggta gcgcggagga tggcatcatt cctcagggaa
     7561 acgccggcgg gccgctgaat ccgagcgagc ccctctacaa ctccatgcct ttcttgctgg
     7621 ccgtcaatac gcagcaggat tcgagcgggc agcccgccta cgcttacggg ctgtttctcg
     7681 acaacgtcgc tcagtcctat ttcaacttag gttccaatga ctactcggac atgtccggga
     7741 agtactactt gggcgcgctg ttcggagacc tcgactacta cattttggtt ggcgagggtt
     7801 ccggcagcca agccattcgc agcgtggtct cccaatatac ggccttgacg gggcgccccg
     7861 ccatgccccc gaagtacgcg ttgggctacc atcaaggatg ttatgggtac tatgatgttt
     7921 cgatcttgct cggggcggcc caagcctatc gcttgagtca gattccgatt gatgggctcc
     7981 atatcgatgt agacttccag aacaactacc gcacgttcac gatcagcccg cgcaagttcc
     8041 ccgcgcccca agccatgttc gaaatcctgc atcaaatggg gttcaagtgc agcgccaaca
     8101 tcaccggcat catctccgcc aatccattgg acgaggacgg gaacaccaac acgccgtacc
     8161 ccgcgcgtga cgcttgggtc acggtgagcc cgagctcgca aattacggtc aaaccaggcg
     8221 tgattccgcc ctttatctat gacacgcgtc agggtgaggc ggagtcgccg aacctgttca
     8281 tcgccaacga gggttacgga gacgaggaca cctccctgta tccatttggt tacaacccgt
     8341 acccgtaccc gacgccgctc tatccccagg gtcaggagtc actcgggaca tacggatttt
     8401 acagtgatat ggggcgaccg gacgtgcagc agtggtgggg ccagcaatat gcgtatctgc
//
LOCUS       DASTKU010000274         5916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1614804, whole genome shotgun sequence.
ACCESSION   DASTKU010000274 DASTKU010000000
VERSION     DASTKU010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614804"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VFQ61_09865"
     CDS             <1..689
                     /locus_tag="VFQ61_09865"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9954801.1"
                     /translation="LLEQAEHGTLFLGDICELSPALQTKLLRFVQERSFRRVGAATDI
                     VSQARVIATSTRNLDDAVRDGLLRPEFAYRLAVVMLEVPPLRERKADIPLLVEHFVAR
                     KASQGARLHAVSEAAMKRLCEHSWPGNVRELFNVLEAAAVVNNAEVIDETQLPLLSAP
                     RPVVDYRLPSQGIAFSELEREVLTQALRLAAGNQTRAASLLGLTRDQMRYRMAKFGLS
                     SRDIAGDRAA"
     gene            complement(924..1757)
                     /locus_tag="VFQ61_09870"
     CDS             complement(924..1757)
                     /locus_tag="VFQ61_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04290 family methyltransferase"
                     /protein_id="HET9954802.1"
                     /translation="MSDLARQIQELGPWFHNLHLPDGTQTCPEHPLGDFPRFKWRKLA
                     PHLPEDLHGATVLDIGCNAGFYSLELARRGAQVTALDVDEHYLAQARWAAQVCGLNQR
                     IRFERKQIYELATTSRTWDIVLFLGVLYHLRYPMLGLDIVSRCVGKTLVLQCLTAPGE
                     EIEFAPSDFPLLDREPLTRPGWPKLSFIEKKMAGDQTNWWAPNRACLEAMLRSAGMRI
                     TQRPQHEFYIAVPDREGASSMWTWNDSEYWAAVGASDSSPARREGEDAHSVPSKTASS
                     G"
     gene            complement(1754..2977)
                     /locus_tag="VFQ61_09875"
     CDS             complement(1754..2977)
                     /locus_tag="VFQ61_09875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9954803.1"
                     /translation="MSRPLQLVILGLSITSSWGNGHATTYRALVRGLAELGHRVSFLE
                     RDMPWYAENRDEHNPEGCTVALYSSIGELEEQHRDMVRNADAVIIGSYVPDGQEVMDW
                     VLSTARGLTLFYDIDTPVTLERLRRGEATYLNRDSVRRLDAYLSFSGGPVLHQIESEL
                     GARLALPLYCSVDAARYVPTGDQPARDFGYLGTYSADRQPTLDRLLTEPARHWSEGRF
                     VVAGPCYPQTEWPDNVQRIEHIAPGDHPSFYCSLRFTLNVTRADMIQRGYSPSVRLFE
                     AAACGVPIISDAWSGLEEFFTPGSEILLPRSGPDVLKILRGMPESERREIGQRARHRV
                     LNRHTSLHRALELEHYVTELQAGRTRGLRSWSVDSGASAPAAIVARRAAELPGAARAH
                     NPSPSQISGARLGDT"
     gene            complement(2974..4059)
                     /locus_tag="VFQ61_09880"
     CDS             complement(2974..4059)
                     /locus_tag="VFQ61_09880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9954804.1"
                     /translation="MGLNIAFFGSSLVSAYWNGAATYYRGVLRALYDRGHRTVFYEPD
                     AYSRQQHRDIADPDYASVVVYTGEDGLSSALEAARSADLVVKASGVGVFDAELERAVL
                     ELRRPGQNVVFWDVDAPATLARVRENPADPFRALIPRYDAVFTYGGGPPVIDAYTALG
                     ARSCVPIYNGLDPATHHPVPADSSFKADLGFLGNRLPDRERRVEHFFLSVAERLPERS
                     FLLGGSGWADKPRPENVRYLGHVYTHQHNAWNSSLLAALNISRDSMAEMGYSPATRVF
                     EAAGAGACIITDAWLGIEHFFEPDREILVARDAEEVARIVSELTPETSRRIGQAALQR
                     VLRDHTYAQRALEFERALGLSIMELAS"
     gene            complement(4050..5249)
                     /locus_tag="VFQ61_09885"
     CDS             complement(4050..5249)
                     /locus_tag="VFQ61_09885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9954805.1"
                     /translation="MIADLHRHKPRILMTTDAVGGVFSYCRALATQLTRRGFEVGIAT
                     MGPAPSHEQREQLRAISGTSVFESDYALEWMDDPWAQVDAAGEWLLAIEREFAPDIVH
                     LNGYCHAALPWRVPKVVVAHSCVLSWWKAVLGEPAPERYGEYAARVQQGLRAADCVVS
                     PTQSFLSSLRGCYSFGSRARVIWNGAESSAAPTSERRACFIAAGRMWDRAKNLTLLER
                     TAAELDWPVFVAGDVGPTPAQPSTENSARQAKREALKFLGRLSQSQLADILSSAAVFV
                     HPARYEPFGLSVLEAALSGCALILGDLPSLRELWADAAIYVDPDDAAGFLQACRALTR
                     DPERRQVLAARAQERAHAYSVERMGAAYQALYLGLLRARADRGRAASTSARLRTLEQS
                     GANTVWD"
     gene            complement(5319..>5916)
                     /locus_tag="VFQ61_09890"
     CDS             complement(5319..>5916)
                     /locus_tag="VFQ61_09890"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9954806.1"
                     /translation="KGAADQYVLDYARSFGLPTAVFRMSCIYGPHQCGNEDQGWVAHF
                     LMRALAGAEITIYGDGYQVRDLLFVEDLVEAMIRARDGMDSLRGQSFNIGGGVDNAVS
                     LREVLAQIRDLLGQEPRVSYGPVRTGDQRYYVSDIGKFRRATGWSPKVSVSEGLSRLY
                     GWLTRAGLPNARERNSLSDLSKELPKPTQARPNEMSAR"
BASE COUNT         1134 a         1956 c         1821 g         1005 t
ORIGIN      
        1 ggctgctcga acaagccgaa cacggcaccc tgttcttggg tgacatttgc gagctctcac
       61 cggcgctgca aacgaagctg cttcgcttcg ttcaggagcg cagcttccgg cgggtgggcg
      121 cggccaccga tatcgtgtcg caagctcgcg tgatcgcgac ctcgactcga aatctggatg
      181 atgccgttcg cgacggactg ctgcgtcccg agttcgctta tcgactcgcg gtggtgatgt
      241 tggaggttcc gccgctacgt gaacgcaaag cggatatccc gctgctcgtc gagcacttcg
      301 tcgcccgcaa ggctagccaa ggagcacggc tgcacgcggt gagcgaagcg gcgatgaagc
      361 gattgtgtga gcactcctgg ccgggcaatg tccgcgagct cttcaacgtg ctcgaagccg
      421 ccgcggtcgt gaacaacgcc gaggtgatcg acgaaaccca gctcccgctg ctcagcgcgc
      481 cgagacccgt cgtcgactac cggctcccct ctcaaggtat cgccttctcg gaactcgagc
      541 gcgaagtcct cacgcaagcc ctcaggctcg cggccggcaa ccagactcga gcagcctctc
      601 tgctcggtct gacgcgagat caaatgcgtt accgaatggc aaagttcggc ctcagcagcc
      661 gtgacatcgc cggcgatcgc gcggcctagc cgacgtcgac cctgcagtcc gcaaaatccg
      721 tttcctcctg gtggagggtc ggcgtccgca agggggatgc cggccctcct ttcgtatttg
      781 cgctcaccgt tcgctacttc gatacccggg aagcccgaga cgcgctgagg actggagtgc
      841 ggaggttccg ggtggagaaa caaaatgaaa gaggctggga gccatgctgt tcccagccct
      901 cgagtcattc cgcctagctg ttctcaacca gacgaggcgg tcttcgatgg cacggagtgc
      961 gcgtcctcgc cctcacgacg ggctggggat gagtccgatg cacccacggc agcccaatat
     1021 tccgagtcat tccaggtcca catgctcgag gcaccttcgc ggtcgggcac agcgatatag
     1081 aattcgtgct gcggacgctg agtgatacgc attcccgctg aacgcagcat ggcctccaaa
     1141 cacgctcgat tcggagccca ccagttcgtc tgatcgccgg ccatcttctt ctcgatgaag
     1201 gacaacttgg gccaaccggg ccgagtcagt ggctcgcggt ccaacagcgg aaagtcggag
     1261 ggcgcaaact cgatttcttc acccggcgcg gtcaaacact gcaagacgag ggtcttaccg
     1321 acgcagcgac tcacgatatc gagtcccaac atgggatacc gcagatggta gagcacccct
     1381 aggaacagca cgatgtccca ggtccgtgag gtcgtagcga gctcgtagat ttgcttacgc
     1441 tcgaagcgaa tccgctggtt caagccacaa acttgggcgg cccagcgggc ttgcgccaga
     1501 tagtgttcgt cgacgtcgag cgcggtcacc tgggcaccgc ggcgcgctag ctcgagactg
     1561 taaaacccgg cgttgcatcc gatatccagc acggtggccc cgtgcaggtc ttcggggaga
     1621 tgcggcgcga gcttgcgcca tttgaagcgc ggaaagtcgc cgagcggatg ctccggacag
     1681 gtttgagtgc catccggcaa gtgcagattg tggaaccacg gcccgagttc ctgaatctgg
     1741 cgtgcgaggt cgctcatgta tcccccagtc gggcgccgga aatctgcgac ggcgaagggt
     1801 tgtgagcccg agccgcaccc ggcaactccg cggcgcgccg cgcgacgatt gcggcagggg
     1861 cgctcgcgcc cgagtccacg ctccagctcc tgagcccgcg cgtgcgacca gcctgcagct
     1921 cagtcacata gtgctccagt tcgagcgcgc ggtgcaagga cgtgtgccga ttcagcactc
     1981 ggtgacgcgc ccgctgtcca atctcgcgtc gctccgactc cggcatgccg cgcaagatct
     2041 tgagcacgtc cgggccgctg cgcggcagca agatctcgct accgggcgta aagaattcct
     2101 cgagcccact ccaggcgtcg ctgatgatgg gaaccccgca cgcggccgct tcgaagagcc
     2161 gaacgctcgg cgaataccca cgctgaatca tgtccgcacg cgtgacattc agtgtgaagc
     2221 gcagggagca ataaaatgag ggatggtccc ccggcgcaat gtgctcgata cgctgcacgt
     2281 tgtcgggcca ctcggtttgg ggatagcacg gtccagccac gacgaagcgc ccttcggacc
     2341 agtgccgcgc cggctccgtg agcaaacgat ccaacgtcgg ttggcgatcg gcactgtagg
     2401 tgccgagata accaaagtct cgagcaggct ggtccccggt agggacgtag cgagccgcgt
     2461 cgactgaaca atacagaggc agagctagac gggcgcccag ctccgattcg atttgatgga
     2521 gcacggggcc gccgctgaac gacagatacg catctaggcg acgaacgctg tcgcggttca
     2581 aataggtggc ctcgcctctt ctcaggcgct cgagcgtaac cggagtgtcg atatcgtaga
     2641 acagcgtcag cccgcgggcg gtgctgagca cccaatccat cacctcctgc ccgtccggca
     2701 cgtacgagcc gataatcacc gcgtccgcgt tgcgcaccat gtcgcggtgt tgctcttcga
     2761 gctcaccgat cgacgaatag agggctacgg tgcagccttc cgggttgtgc tcgtcccggt
     2821 tctccgcata ccacggcatg tctcgctcca agaacgagac acggtgtccc agttcggcga
     2881 gaccacgcac caacgcacga tacgtggtcg cgtgaccatt gccccaggat gaggtgatcg
     2941 atagcccgag tatgaccagc tgcagtggac ggctcatgat gcaagctcca tgatcgacag
     3001 tccgagcgct cgctcgaact cgagtgctcg ctgggcgtag gtgtgatccc gtagcacgcg
     3061 ctgcagggcc gcctgcccaa tgcgccggct cgtctcgggc gtcagctcgg agacgatgcg
     3121 agcgacttcc tcggcgtctc gcgcgaccag aatctcacga tcgggctcga agaagtgctc
     3181 gattccgagc caggcatccg taatgatgca ggctccggcg ccggccgcct cgaagacgcg
     3241 tgttgcgggc gagtaaccca tctccgccat gctgtcgcgg ctaatgttca gggcggcgag
     3301 cagcgagctg ttccaggcgt tgtgctgatg cgtgtagacg tgtcctagat accggacatt
     3361 ctcgggccgc ggcttgtccg cccagccgct gcccccgagc aagaagctac gctctggcag
     3421 ccgttcggcc acgctcaaga agaagtgctc gacccgacgc tctcgatccg gcagacgatt
     3481 gcccaagaac cccaggtctg ctttgaagct cgaatcggca gggacggggt ggtgagtcgc
     3541 gggatcgagt ccgttgtaga tcggcacaca gctccgcgcg ccgagcgcgg tataggcatc
     3601 aatcacgggc ggaccgccgc cgtacgtgaa caccgcgtca tagcgaggga tcagggcccg
     3661 aaacggatcg gcagggttct cgcgcacccg agccagggtc gccggcgcgt cgacatccca
     3721 gaacaccaca ttctggccgg gcctacggag ctcgagcacg gcgcgctcca actcggcgtc
     3781 gaatacgccc acgccactgg ccttcacgac caggtctgcg ctacgcgctg cctcgagcgc
     3841 gctcgacagc ccatcctcgc ccgtataaac cacgaccgag gcgtagtcgg gatcggcaat
     3901 gtctcggtgc tgctgtcgcg agtaggcgtc gggttcgtag aacaccgtcc ggtggcctcg
     3961 atcgtagaga gcgcgcagca caccccggta gtacgtcgct gcgccattcc aatacgcgga
     4021 gactagactc gagccaaaaa aagcgatgtt caatcccata ccgtgtttgc tccggactgt
     4081 tcgagagttc gtagtcgagc cgacgtgctc gcggcgcgcc cgcgatccgc ccgcgcccgc
     4141 agtagcccga ggtagagggc ctgataagcc gcacccatac gctcgaccga gtacgcgtgt
     4201 gcccgctcct gagcgcgagc ggcgaggact tgccgccgct ccggatcccg ggtgagcgca
     4261 cgacacgcct ggaggaagcc agccgcgtcg tccggatcga cataaatcgc ggcgtccgcc
     4321 cacagctcgc gcagactcgg caaatcaccg aggatcagag cgcacccgga caaggccgct
     4381 tcgagcacac tcaacccgaa cggctcgtag cgcgcgggat gcacgaacac tgccgcgcta
     4441 ctcagtatgt ccgcgagctg cgattgagag agccggccta gaaatttcag cgcctctcgc
     4501 ttcgcttggc gcgccgagtt ctccgttgac ggctgcgcgg gagtcgggcc aacgtcaccc
     4561 gccacgaaca cgggccagtc cagctcggcc gcggtccgtt cgaggagagt caagttcttc
     4621 gcgcggtccc acatccgccc tgccgcaatg aagcaggcgc gccgttccga cgtgggcgcc
     4681 gcactagact ccgccccatt ccaaatgacc cgggctcgag atccgaacga gtagcacccg
     4741 cgtagactgg acagaaatga ctgggtcggc gagacgacgc agtccgccgc gcgaagccct
     4801 tgctgcactc gcgcggcgta ctcgccataa cgttcgggtg caggctcacc cagcaccgcc
     4861 ttccaccacg aaaggacgca cgagtgcgcg acaactactt tggggacgcg ccacggcaat
     4921 gcggcatggc agtagccatt caaatgaacg atgtccggag cgaattcccg ctcgatcgcc
     4981 agcagccact cacccgccgc gtccacctgg gcccaggggt cgtccatcca ctcgagagcg
     5041 taatcgcttt cgaacacgct cgttcccgaa atggcgcgca gctgctcgcg ttgctcatgg
     5101 gacggagcgg gacccatggt cgcgatcccc acctcgaaac cgcgtctcgt gagctgagta
     5161 gccaatgccc tacagtagga gaacacgccg ccgaccgcgt ccgtcgtcat caggatacga
     5221 ggcttgtggc gatgcaagtc agctatcacg gggcacctgt gcagttagct cgaggcgacg
     5281 gacgaacacg cgcaggattc gaccgtctcc aattcctttc aacgcgccga catctcgttg
     5341 gggcgggcct gcgtcggctt gggcagctcc ttgctgaggt ccgacaacga gttgcgctcg
     5401 cgagcgttcg gtagcccggc tcgggtaagc cagccgtaca agcgagacag cccctccgag
     5461 accgacactt tgggagacca gcccgtggcc cgccggaact tgccgatatc ggagacatag
     5521 tagcgctgat cacccgtgcg caccggcccg tagctgacgc gcggctcctg ccccaacaga
     5581 tctcgaattt gcgcgagtac ctcacgcaag ctcaccgcgt tgtctacccc gccgccgatg
     5641 ttgaaggact gtccacgcag cgagtccatg ccatcgcgcg cgcgaatcat ggcttccacc
     5701 aggtcctcca cgaacagcaa gtctcgaacc tggtagccgt ctccataaat cgtgatctcg
     5761 gctcccgcca acgcccgcat caggaagtgg gcaacccagc cttgatcctc gttgccgcac
     5821 tggtggggcc catagatgca gctcatgcga aacacggccg taggcagccc gaagctccgc
     5881 gcatagtcga gtacgtactg atccgcggcg cccttc
//
LOCUS       DASTKU010000275         4754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1615109, whole genome shotgun sequence.
ACCESSION   DASTKU010000275 DASTKU010000000
VERSION     DASTKU010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615109"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1046)
                     /gene="fabD"
                     /locus_tag="VFQ61_09895"
     CDS             complement(102..1046)
                     /gene="fabD"
                     /locus_tag="VFQ61_09895"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET9954807.1"
                     /translation="MIAWLFPGQGTQTVGMGKSLCAASPAARAVFEAADRALGERFTE
                     LVFEGPLEKLTYTANAQPAILTASMAALAALREAAPALPLPYAAAGHSLGEYSALVAA
                     GALAFEQAVQLVRLRGEAMQRAVAAGEGAMAAIMGSDPGAVEQLCADVRAELVGRVVS
                     PATFNAPGQIVIAGHADAVARAASLGKERKLKAIPLKVSAPFHCSLMAPAAEVLDRAL
                     AEVRFGPLGFPVVSNVDASANRDSAKIRSLLVRQVAGAVRWQESLQWMAAQGVSAALE
                     IGPGNVLAGLAKRGVPSLVVSPAFEASEISAVAQRGRA"
     gene            complement(1075..1296)
                     /gene="rpmF"
                     /locus_tag="VFQ61_09900"
     CDS             complement(1075..1296)
                     /gene="rpmF"
                     /locus_tag="VFQ61_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET9954808.1"
                     /translation="MPVPKRRTTSSKRDMRRANHDKVAPPNLTPCSNCSAPTLPHRVC
                     PSCGHYAGRAVVSQREAASGSGESDSASS"
     gene            complement(1320..1940)
                     /locus_tag="VFQ61_09905"
     CDS             complement(1320..1940)
                     /locus_tag="VFQ61_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HET9954809.1"
                     /translation="MASPLFTVSVRELERAEQRRRFELPLEWLDWAFAETEAKSDGQP
                     GTLEVHLKKNGREVLVKGTARAHVTMPCARTLDPVPVELTAEILLLLSPRAPAPVRAG
                     GRKARRERPSEPAETAGSPQEESLSSELAARDEYDGDHIELDGFVREYLLLELPLFPL
                     RSDLPFDPTPATDPRPNDRRPAAPGDTVDPRLLPLAALASQFKKAE"
     gene            2133..3467
                     /locus_tag="VFQ61_09910"
     CDS             2133..3467
                     /locus_tag="VFQ61_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954810.1"
                     /translation="MRAWLRTERGRTWLCAFGCYVVLLGIYAFIAGANRLTEHTPYNH
                     FALLAEAWLHGRLDLGGPPPDYAGNNDFALYQSKWYVPFPPLPAALILPLVYLAGSAD
                     RVRDAQFFVWLAPLGPALFFVVLETLRQRGRSVRASWQNLTLALSLALGSVYFFTAVQ
                     GSVWFAAHVVALACVAGYLLFAIDARHPWLAGLSLGLAFWARSVLMFGGAFFLCEAWR
                     VCRSATEDRSLARTESTWRRLRNAGRETDVRRYLLLCARFSLPLLALFALQLAHNYAR
                     FGDPLDVGYRHLQIAWQGRIHKWGLFHYHYLAKNLGVVLTSLPWIKPFRINDHGLALW
                     LTTPLYLLLLWPAKPRAGFVGLVLTAACVGIPELFYQNTGWMQFGYRFSNDYAPFLFA
                     ALALSGRRFGLCFWVFAVWGVAVNTFGAITFDRAAYAHFYYEQASQETLYQP"
     gene            complement(3886..4269)
                     /locus_tag="VFQ61_09915"
     CDS             complement(3886..4269)
                     /locus_tag="VFQ61_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954811.1"
                     /translation="MTSPYGAITIARNQSLTEQRITLYHELVHRYFSPRTGPLRRLRA
                     EVRISGYSRSALLRYLEEALAEGYGQLRGYGLDKALAAYRFPIDFGYVTVSRMMAEGQ
                     AIGSITLGGVLFYVSVSQGPIPRQK"
     gene            4362..>4754
                     /locus_tag="VFQ61_09920"
     CDS             4362..>4754
                     /locus_tag="VFQ61_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954812.1"
                     /translation="MRFASRDERTALTTQKHGLDRRYAQQCDRTREMLACFVQLSGRM
                     SVPGGGHELQELVCALKDCKADRDRSNAKEDDVHDFANAEEVRPRPYDQRTSNESDSR
                     WPEQALHNSSYLRWQVGQRPSSPPQAFRP"
BASE COUNT          819 a         1612 c         1454 g          869 t
ORIGIN      
        1 gctgtgtacc acgcttgccg gcgaggcaag cagaagaatt tccgtccggc cgctttgcgc
       61 ttcgcgtcac gcgcaaccaa gccgtctgga gccgtgcctc atcaagcccg gccgcgttgt
      121 gcgacggcag agatctcgct cgcttcaaac gccggactga cgaccaaaga gggaactcct
      181 cgtttggcga gaccagccag cacgtttcca ggaccaattt ccaaagcggc cgagacgcct
      241 tgggccgcca tccactgcaa gctttcctgc cagcggacag ctcctgcgac ctgacgaacg
      301 aggagcgaac ggatcttcgc cgagtcgcgg ttggccgaag catcgacatt gctcacgacc
      361 gggaacccca atggcccgaa ccgcacttcg gcgagggctc gatcgagcac ctccgcggcc
      421 ggcgccatga gcgaacaatg gaagggagcg ctcactttga gcgggatcgc tttcagcttc
      481 cgctccttgc ccaggctcgc agcccgcgcg accgcgtccg cgtggccggc gatcacgatc
      541 tggccagggg cattgaaggt ggcgggggac accacacgcc cgacgagctc tgcgcgcaca
      601 tccgcgcaca gttgctcgac tgcgcccgga tccgagccca tgatcgcggc cattgcgccc
      661 tcgcccgcgg cgacggcccg ctgcatcgcc tcaccgcgca agcgcacgag ctggaccgct
      721 tgctcaaacg ccagcgcacc ggcggccacc agcgcgctgt actcgccgag agaatgcccc
      781 gccgccgcat acggcaaggg cagcgcggga gctgcttcac gcaacgccgc cagcgcagcc
      841 atgctggccg tcaaaatggc cggctgcgcg ttggccgtgt acgtgagctt ctccagaggc
      901 ccctcgaaaa cgagctccgt gaagcgctcc cccaaggctc gatcggctgc ttcgaacacc
      961 gcgcgagcag ccggcgacgc cgcgcacagg ctcttgccca tgcccacggt ttgggtccct
     1021 tgacccggaa ataaccacgc gatcacgagc gtgcccgatc agcgtccagc ctgatcagct
     1081 ggatgcggaa tcgctctcac cgctgccgga tgccgcttcg cgctggctca cgacggctcg
     1141 ccctgcatag tgaccgcagc tggggcaaac tcggtgcggc agcgtcggtg ccgagcaatt
     1201 ggagcagggc gtcaagttcg ggggcgcgac cttgtcgtgg ttcgcccgcc gcatgtcgcg
     1261 cttgctgctc gtggttcgtc gcttgggtac tggcaccgct ctaactcctc gtccgcctgc
     1321 tactcggctt ttttgaactg gctcgctaga gcggccagtg gcaacaaccg cgggtccacg
     1381 gtgtcccctg gggccgctgg ccgccgatca tttggccgcg gatcggtagc gggggtcgga
     1441 tcgaaaggca agtcggatct gagtggaaag agcggcaact cgagcaaaag gtactcgcgg
     1501 acgaagccat ccagctcgat gtggtccccg tcgtattcgt cacgagcggc caactcgctg
     1561 gacaagctct cttcctgcgg ggaaccagca gtctcggccg gctcactcgg ccgctcacgg
     1621 cgcgccttgc gacctcccgc gcgcaccggc gcgggcgccc gtggactcaa tagaagcaga
     1681 atctccgccg tcagctccac cgggacggga tcgagcgtac gcgcgcaggg catggtcacg
     1741 tgcgcccgcg cggtcccttt gacgagcacc tctcgcccat tcttcttcag atgcacttcg
     1801 agcgtcccgg gctgtccatc gctcttggcc tcggtctccg caaacgccca gtcgagccat
     1861 tcgagcggca gctcgaagcg acggcgttgc tccgcgcgct ctagctcgcg cacggaaacg
     1921 gtaaatagcg ggctcgccat gccgccaacc tagcacgtgc gcatgcgccg tgtccgaaac
     1981 cggatttggc tcgcgcgcga acctcggctc gctagtcgga caggacacgc tgctcggagc
     2041 gcagtccagg cgttaccgcc ctgcggcggg cagacggcac gaaatgccga aggtttcgca
     2101 atccgtagcg agtcggatag ggtcgccgcc acatgcgagc ttggctcaga acggaacgcg
     2161 gccgcacctg gctttgtgcg ttcggatgtt acgtggtgct gctcgggatc tatgcgttca
     2221 ttgccggagc gaaccgcctc acggagcaca ctccgtacaa ccactttgcc ctgctcgccg
     2281 aagcatggct gcacggcaga ctcgacctcg ggggaccacc gcccgactac gcgggcaaca
     2341 acgacttcgc gctctaccag agcaagtggt acgtgccctt tccaccgctg ccagccgctc
     2401 tgatcctacc gctggtttac ttggcaggca gcgcggatcg ggtgcgcgat gcacagttct
     2461 tcgtctggct cgcgccgctg gggcccgcgc tgttcttcgt cgtgctggag acgctgcggc
     2521 agcgcgggcg cagtgtgcgg gcctcctggc aaaacctgac gctggcgctg agcctggcgc
     2581 tcggcagcgt gtacttcttc acggccgtcc agggcagcgt ctggtttgcc gcccacgtgg
     2641 tggcgctggc atgcgtcgca ggatatctgc tcttcgccat cgacgcgcgc cacccctggc
     2701 tcgcggggct ctcgctcggc ttggcttttt gggcgcgctc ggtgctgatg ttcggcggag
     2761 cgttctttct ctgcgaggcc tggcgggtct gccgcagcgc gaccgaagat cgatccctgg
     2821 ctcgaaccga gtcgacctgg cggcgcttgc gaaacgctgg gcgtgagacg gacgtgcgcc
     2881 gctacctgct gctttgcgcc cgcttcagtc tgcccctgct ggcgctcttc gccctgcagc
     2941 tcgcccacaa ctacgcgcgc tttggcgacc cgttggatgt cggctaccgg cacctgcaga
     3001 tcgcttggca ggggcgcatc cacaagtggg gcctcttcca ctaccactat ctcgcgaaga
     3061 acctgggcgt cgtcctcacg agcctgccgt ggatcaagcc gttccgcatc aacgatcacg
     3121 gcctcgcgct gtggctgacg acgcccctct acctgctatt gctctggccc gccaagcctc
     3181 gagccggctt cgtagggctc gtactgaccg ctgcctgcgt cggtattcca gagctctttt
     3241 accagaacac gggctggatg cagtttggct atcgcttctc gaacgactac gcgccgttcc
     3301 tgttcgcggc cctggccctt tcgggccgcc gcttcggcct ctgcttctgg gtgttcgccg
     3361 tctggggagt cgcagtcaac accttcggcg cgatcacctt cgaccgcgcc gcgtacgcgc
     3421 atttctatta cgaacaggcg agccaggaga cgctgtatca gccgtagggg agggagggct
     3481 ccgccatcgg cattcaggca gccagcggca gagctccgca ataccctccc gtgctgcaga
     3541 tgagggcgag acgcttgtaa cttgtacgtc gtgcgcagtc gtgaactctg aacgcaggca
     3601 acttgcgaac tgcgcgacca atgcctgagg gcacgatcag caatgacttg tcgagcccgt
     3661 ttgcgcgtca ccgaggtatg taaccctcgg aaagaacctc gccagtctcg gcatctacag
     3721 tgaatctggc cttggtcccc agctgacccg cgttcgtttc gatctcaaag cggtcatgct
     3781 gaccaaacca accacggcga tgcacgaccc acagcggctc accgaaactc cagccattca
     3841 gcgcagcgcg ctggcgtgcg atctcaattg ctctcgtttg gtcgatcatt tctgtctcgg
     3901 aatcggacct tgcgagaccg agacgtagaa gagtacgcca ccgagcgtga tgctcccgat
     3961 ggcctggccc tcagccatca ttcgggacac cgttacatac ccgaagtcga ttggaaatcg
     4021 atacgcggca agcgccttgt cgaggccgta cccccggagt tgcccgtacc cttccgcaag
     4081 ggcctcttct aagtatcgaa gcaacgccga gcgcgagtac cccgagattc tgacctcggc
     4141 tcgaagcctg cggagcggtc cggtccgagg cgaaaaatag cgatgaacaa gctcgtgata
     4201 cagcgtgata cgttgctcag tcagcgattg attgcgcgcg atagtgattg cgccgtaagg
     4261 cgacgtcaat ccggcatctc cgccaggaat atgggttgga cgtgcaggcg gagtgcatct
     4321 gctcctggcg gcgccgcctc catttcgatt cgagggtaga tttgaggttt gcctctcgcg
     4381 atgagcgcac ggccctgacc acgcagaagc acggcctgga tcgtcgatat gcccaacaat
     4441 gtgaccgcac gcgcgaaatg ctggcctgct tcgtccagtt gagcggtcga atgagcgtcc
     4501 ccggcggtgg tcacgaactt caggagctcg tctgcgccct caaagactgc aaagccgacc
     4561 gcgatcgctc caacgccaag gaggatgatg tccacgattt cgccaacgcc gaagaagtgc
     4621 gaccccgccc atacgatcag cgtaccagta acgagtccga tagtcgctgg ccggagcagg
     4681 ctctccacaa tagttcctac ctccgctggc aagtggggca acgaccctca tcgcctccgc
     4741 aagcctttcg gcct
//
LOCUS       DASTKU010000276        13304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_500755, whole genome shotgun sequence.
ACCESSION   DASTKU010000276 DASTKU010000000
VERSION     DASTKU010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13304
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500755"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VFQ61_09925"
     CDS             complement(<1..1076)
                     /locus_tag="VFQ61_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954813.1"
                     /translation="MAIAAGCQTTDNPAGGEPAATGGAHATPDETAGAGGQGTAVESP
                     PNGMKDDCADGWVESENTGDGRTTYSLHGVLPRPSLKGPTYPTCGDSPLRGAQRVYRL
                     DLGRQPGRFKLYAALDAEFGGALVIGRGDCADLEDLICTRGVNQPKEARLSAELDPGR
                     YFVVVRGAGLEDYGSYELQVTVEPLEADCRPASNKDCASATPLATDGRLLRTYLPVDC
                     DYEATNEVQESRFYALDLRAEPGTTGLHAQLDFVEEAPPEDSDTEYPTLRLWGLDPSG
                     ACSATQVADMGLFSQYGRSVSTALAPGLYALELESPPARPAYLRVEFHRPDCSGNVHD
                     TCANAEDIQLAGEQATVRGHTYCGR"
     gene            complement(1121..2836)
                     /locus_tag="VFQ61_09930"
     CDS             complement(1121..2836)
                     /locus_tag="VFQ61_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954814.1"
                     /translation="MQCTRRLVVYLIGWLMLGCAPAHPSTEEPERLVRADPPAIVPPA
                     PQCPVRVCAGACTSSAEGTEYTKLATCNEPLPLIARGSLQSGLAERVVTGHKLCGTSY
                     SSGAECGLDGEALTFELDLRDSPREVDLLVEFDAEFDPTLRLETGACEDPQVVACNRN
                     HTEQVERAVLSQRLKPGLYHLVAGAEGQHSEGGASAEGAFRLAVTARDPEARCQRPPP
                     NDTCATATPLDPTLEFRTLVGSTTCARDDAWSTLLCAYDNAPDVFYRIELPEELGPRI
                     LRASTDLPETNFDTVLYVLEGSHGHCFDVLACNDDVPEPVIASSPSEVSILLDPGEYY
                     LVVDGSYDTGDFGLNIEFSAPECLRNDDCSRALPLDPSFGNQTAPIDVTCATSTRVVE
                     SYEDDQPKDAFYRLDLSAATGLVRLNVDTDLDDVFGFSAGLIAEERPGICGQTLLTKG
                     FWRNGAVVEPGIYYIVISVPLEEWQRGNLEVEIEPVSEDELTSCVDAAMAADANLYCS
                     DDWEMNFMQFSACGLAPALQSCVCEAAPRCCTPAGRFAECIPAFEACNLFCPDFDLQA
                     YCEDA"
     gene            3156..4295
                     /locus_tag="VFQ61_09935"
     CDS             3156..4295
                     /locus_tag="VFQ61_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954815.1"
                     /translation="MTELAAGWRHVCVVFEDQRARCWGQNDRGQLGLGNKDTYGDDPT
                     ETLAALPDLPLERVSRISAGVHNTCAIVAASSAAPGQVHCWGSDQDGALGDRGSGDFG
                     DDEPVDNRRPVALPENALQIAVGGEFACALLVTGALHCWGDNWAKTLGIGDSTCDIGD
                     ERPCIGELGRSPNIPVGRLGTRPIVKLRASQSSACVVDNTGALRCWGRNSQSRLGYPE
                     LVQGEFLGAPPEPVELGSGVTIVDVALGLRHACALDSMGQVRCFGEQGPALGYGMLGE
                     EGVAGIGGIQTPASAYALREDRGIVDLGDLDGVAGSDRAQAIFAGGMSTCARMLDGGV
                     RCWGANSGGELGYGAYDAVGAIGDDSSPADEYVRLGVRANIFAEP"
     gene            4587..5450
                     /locus_tag="VFQ61_09940"
     CDS             4587..5450
                     /locus_tag="VFQ61_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954816.1"
                     /translation="MLLCQNRLGKFKGLSFARAFSLLSLPLCLGACGAVDPSAAASLE
                     DDQAATATESAALTADDGYVQSITLRMVGGDPVQTAYTHNGSRVQLDTTPSAGKATLD
                     APPAPGAQHRGKATLRVTCVNPNGVAIVKTQGGLYNLVGVGKATFTAPCDPNYRAAKS
                     ELILNPSDGIGDTVNYLQLAPSPSADRDFSIQIKVPGVAAATSSRNFVKYGYLTLNTA
                     QGSAWFRYFNASNDARNVLMSYRIQCSGTWTDTVTRDAVVGPQLPTNFVLSCPAGTVL
                     ERVDTRLQNIR"
     gene            5564..6097
                     /locus_tag="VFQ61_09945"
     CDS             5564..6097
                     /locus_tag="VFQ61_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954817.1"
                     /translation="MLRRSEPFRSAGVLEVVMPFGVHGSMKYLILFLPLVTLGHCKGR
                     DADEPAMLPDTERVSAASSTKLLQSSASPVALPAAPATGFASAPKATAEPGFCEVMCG
                     HTRALGCTALSECSASCTALLADQQCHDQLIHALSCMIAEPRTHWVCGEDGLAAIDSG
                     YCDAEQRAFVGCVQQAS"
     gene            6238..10986
                     /locus_tag="VFQ61_09950"
     CDS             6238..10986
                     /locus_tag="VFQ61_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HET9954818.1"
                     /translation="MQYASSISKRHLRIGFVGSASAFLLAMALSGCSSVTPSSTSDQE
                     TVSVAEQALTTSFSVITLSGNPNDTILSATGKLSIDSRVDIGDGSKLETVSSFGAGSA
                     NQTFLQSAAVIHGNLTSVAPVKLESQAKVEGFARSAGTITKDAGATIAVEYPSATNIT
                     SQTTSWNVNFPDTDGGAVTVNTLEQRTLAPGAWGDVQLIGGDTVEQGAKLTLTSGTYF
                     FKSFVSNPKTQIFLNKSAGPIYIYVKEKFTYKGGFVTTVGVATETLVAALGTDAAGSG
                     EAVVYLEAPMTGTVVAPNGTIDLRRPPNDQPHRGAFFGKNIHVFSDIDVYHAPFDFRC
                     SLGDQDRDGTDDCTDACPLDPSKTAPGLAGCGKSETDADGDKLPNVIDGCKNDAKNQV
                     EGQCGCAGSSAAPVGTPCTDSVCPSATNTTCDGAGVCGDPASCAPAASGCTVRRFQNS
                     IYWFCTSPVTWSQAASNCRAVSGRYLARINSRVENDFFAQSLGGKAWHGGNDSSTEGT
                     WRWSSASSNNGDPFWSGDGTGSSTSGRYAKWAQGQPTTANDCGAVQPASSGGQWTAEA
                     CTGALAYVCEQPVNFAPPPVRDLDLGKFFPERGGSTPPQQATSCVPEATALPWTKAQA
                     EQQITACNAAFNNGTCTETQSAGCSAACKGIATVPPAGSTGSCSKYTPDELAQCALRN
                     VVEAGCTAGVDCCSYVENEILSQSFETSVGTFTYVDDAFLGTTQPSYAAGQRVTSGGN
                     PGAAAQVTLGGIDNTLITNMSGGWRSNFTVPAVSDVILSFDYNLTQSPNYESSDISQV
                     MVRVDGVVKGLNLRDHVEQIAGNGDGGANISTGWRSARLNLGDLSAGPHVFSIGAYNN
                     AKNNANETTTLLIDNVRLIARDDTCAAGQICGPVYSSGCNPCDTVDANGKCTKNCPPP
                     VLRCGTRTAKCADTSISGSTCSMVELCSGPEATGSSDPRTNGDLTASPAFDPVTEFGQ
                     PTAPATKYYADPACETPPCSAGFNNRWCRYEPKIWNDPVVKRMNPEVIDPMPAKATRP
                     DTKRGESGSSALKFVFDPDAHLDYSLDPLGGGQTNFYLDTSASLLSTVGFDIGSDPLR
                     VKAKVDIVDALVKLHLDRCHATTLESKLVLFGDDFLPSLAPQLLFDEQIPNCEAAIDE
                     YGKALNRVRKAYKDAMELVRQYKARKSAAEKFPADFCQKLTAELPDFFPAGSCATETA
                     AATVNRFIDYYESEIKRVLEPANVQLTNKVLSVSGTPINVPSTPTRESETLVEVTFML
                     GPVPMRLEVEAFVEYGIGGNLRFELNPNALLANTGERETLANVDAHVDPYALSGITMF
                     VGAGFGWGDFGVSAGVEGAVTLGRLSLDNYAGAGVAVQVTKDDREFPSDVKAAVDQAT
                     ADGRLTLFPPAGAKKYDFSLQYNYGSKIVISEVLKGAIAGRVRLKMAFFSKEWRKVLF
                     TFPGFGPFEVPLISGGGNAPAFSIDPHKWGSFQVPFPFAKLARLNPLEVLTGTARAFD
                     TSNVEEPFYDNLCHCSNTAEPCTRRADCCDSANQICFSDPAVGGQGVCSACRQRAQTC
                     NSVADCCPGRGVTCSAPLGSTTKVCGVTAF"
     gene            11029..12999
                     /locus_tag="VFQ61_09955"
     CDS             11029..12999
                     /locus_tag="VFQ61_09955"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9954819.1"
                     /translation="MQSKIGVRGSLRSTVFRVVLVSLPLLAASGAGCSGDSKTADRGE
                     TGTPLQTELTANYARASVYEVELEAESEVGPGQALQLRVVGKLEAHPIPAARGQQGLV
                     LRMRDARAFSGAGATQEIQELSREIARAFFVTMEKGQTRELRLARGMSAAAASVQRTL
                     AAALQFSKPSGGNSWKGDEFDATGKYEVAYELSRAGAGASASAHVFSKKKLGYRALSA
                     NRTQLPAHVQVQSAQVSAQPDPSRSLNLHVDVRESDGEVALGPNLEIERVHYRERLEA
                     KLFQGAPVASKTTLTLTRLTGASPTAAPKETESVSGLVTLRGDAPYPAAAPVDLDRAR
                     TSGLTFEQVVAELETAEQGWAPPDNGRPTEKESPADAAARDRAQRLSKHFVSLEAMFR
                     RQPATVPLAIGKILAKSPAAAVITNALGSSGAPSAQPALLGLAKNRSVEDKVRAIAAN
                     SLIRLARPSEETVTALMTVVDDPVVGVHAVYALGSFARKLREDEPQLAERTARFLVSR
                     LSAVKDSAQKIHVLRGIANSAYVGAIPAVREVLSSKDALLRSAAVESLRLMQGPEVDA
                     LIADRIQDERKSVRNAALSTARVRAPSNELIRAVEGVAIRADDGYGRMEAVRLMGSWL
                     PRAPALREALLRIAQRDEEPRIRETAQKLIDG"
     gene            complement(13018..>13304)
                     /locus_tag="VFQ61_09960"
     CDS             complement(13018..>13304)
                     /locus_tag="VFQ61_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954820.1"
                     /translation="SWSKENAALEADRNRALEMSQIPSLDPSLRQRASANDDLLSSIR
                     LVDAVDVAMLTSGALALGVGAYLTLAPSPGSKPVQLRAASPGQFRLEYQF"
BASE COUNT         2521 a         4017 c         4281 g         2485 t
ORIGIN      
        1 cggccgcagt aggtgtgccc gcgaaccgta gcttgttcgc ccgccagctg gatgtcctcg
       61 gcgttggcgc aagtatcgtg tacgttgccg gagcagtccg gacgatgaaa ttccaccctc
      121 agatacgcgg ggcgagccgg cgggctctct agctccaatg catacagacc gggcgccagc
      181 gccgtactca cggatctccc atattgcgaa aataggccca tatcggccac ctgagttgcc
      241 gagcaagcac cggaagggtc gagcccccat agccggagcg tgggatactc tgtgtcggaa
      301 tcctcgggcg gagcttcctc gacaaaatct agctgcgcgt ggagccccgt agttccaggt
      361 tcagccctca agtcgagtgc atagaagcgc gattcttgga cctcgttggt cgcttcatag
      421 tcgcaatcaa cgggcaaata ggttctcagt aggcgtccgt cggtcgccag cggcgtggcg
      481 gaagcacagt ccttgttgga agcagggcgg cagtctgctt ccagaggttc gacggttacc
      541 tggagctcat aggagccata gtcctccaaa cccgctccgc gaaccacgac gaaataccgt
      601 ccgggatcga gctccgcgct gagccgcgcc tctttgggct ggttcacgcc tcgagtgcag
      661 atcagatctt ctagatcggc gcaatcgcca cgaccgatca cgagcgcgcc cccaaactcg
      721 gcgtccaagg cggcgtataa cttgaatcgg ccaggttgcc gtccgagatc gaggcggtag
      781 acgcgttgcg cgccgcggag aggtgagtct ccgcaagtcg ggtaagtggg gcccttcaag
      841 gaggggcggg ggagaactcc gtggagggag tacgtcgtgc gcccgtcgcc cgtgttctcg
      901 ctctccaccc agccatccgc acaatcgtcc ttcatcccgt tgggcgggga ttcaaccgcg
      961 gtcccttggc cccccgcgcc cgcggtttcg tccggcgttg cgtgggcgcc cccggttgcc
     1021 gccggctccc cacccgcggg gttgtccgta gtttggcaac cagcagcgat tgccattacc
     1081 aggagcgcgg ggcccaaggc cattccaaag tgcctgttcc tcatgcgtcc tcgcagtagg
     1141 cctggagatc gaaatctggg cagaacaggt tgcaggcctc gaacgcgggg atgcactctg
     1201 caaatcgacc cgctggcgtg caacaccttg gtgcagcctc acagacgcac gactgcagag
     1261 caggagccaa gccgcaggcg gagaactgca tgaagttcat ctcccaatca tcggagcaat
     1321 acaggttcgc gtccgccgcc atggcagcgt cgacgcagga agtcagttca tcctcggaca
     1381 caggttcgat ctcgacctcc aggttgcccc gctgccactc ctcgagcggc acggagatca
     1441 cgatgtagta gatgccaggc tccaccacgg cgccgtttcg ccaaaaaccc ttggtcagta
     1501 aggtctgccc gcagataccg ggccgctctt ctgcgatcag gcccgcggag aaaccgaaga
     1561 catcatcgag atcagtgtcc acattgagtc gcaccaatcc agtcgcggcc gacaggtcca
     1621 aacggtaaaa tgcgtccttc ggttggtcat cctcgtaact ttctacgact cgagtggagg
     1681 tggcgcaggt tacgtctatc ggagcggtct ggttgccaaa cgagggatcg agcgggagcg
     1741 ctcggctgca gtcgtcattt cgcaggcact ccggggcgct gaattcgatg ttcaagccga
     1801 aatcacccgt gtcatagctc ccgtctacca ctaggtaata ctcaccgggg tcgagcagaa
     1861 ttgagacttc ggaaggactg gaagctatca ccggctccgg cacgtcgtca ttgcatgcca
     1921 gcacgtcgaa acagtgccca tgtgaaccct cgagtacgta taagaccgta tcgaaattgg
     1981 tttccggcag atcggtagag gcgcgaagga tgcgagggcc cagctcttct ggtaattcga
     2041 tccggtaaaa gacatccggc gcattgtcgt aggcacaaag aagagtactc caggcgtcgt
     2101 ctcttgcgca ggtcgtgctc ccaaccaggg ttcggaactc gagcgttgga tccagaggag
     2161 tagcggtggc gcaggtgtcg tttgggggcg gtctttggca acgcgcttca ggatcgcgtg
     2221 ccgttacggc gagtcgaaac gctccttcgg cgctcgctcc cccctccgag tgttggcctt
     2281 cggcgcccgc taccaggtga tagagccctg gcttcagtcg ttgcgagagc accgcccgct
     2341 cgacctgttc ggtgtggttg cgattgcacg ccaccacttg cggatcttca caagcgcccg
     2401 tttccaggcg aagcgtcggg tcgaactccg cgtcgaattc gaccagaagg tcgacctccc
     2461 ggggtgagtc ccggagatcc agttcgaaag tcaatgcttc cccatcgagc ccgcactccg
     2521 ccccgctcga gtacgaggtg ccgcaaagct tatgaccggt gactacgcgc tccgcgagac
     2581 cggactgcag cgaaccccgc gcaatcaagg gcaaaggctc gttacaagta gccagtttgg
     2641 tgtactccgt gccctcggca ctgcttgtac aagcgccggc acagactctc actgggcact
     2701 ggggagcggg cggaacaatc gccgggggat cggccctgac gaggcgctct ggctcttccg
     2761 tcgaaggatg tgcgggcgcg cagcccagca tgagccagcc aatcaagtac accaccaagc
     2821 gacgcgtgca ttgcacggtc gcgatattta accgatactc tcgcctcgcg atagtgagac
     2881 ttactttaaa tcttgggctt tcgattttgc tccttgcctg tcatggaggg cagtcattcg
     2941 acttcaagga tccaccgggt tgcgggctcg attgtgctcc cgcctccgag ggcacgaaac
     3001 tcgccctagg tctcggtttt tcatgcagtc tgcgtctcgg agaggtgtcc tgttggggga
     3061 atggcacccc tgaaggcggt aaccccttct ggttaccaaa gggtgcttac tctccgggag
     3121 ctgcgggaac tctggacttt ggcacgtctc gccgtgtgac agaactagcc gccggttggc
     3181 gtcacgtctg cgtcgtgttc gaggatcagc gcgcgcgttg ttgggggcag aacgaccgcg
     3241 gtcaactggg tctgggcaat aaggatacgt atggggatga tccgacggag accttggccg
     3301 cgctaccaga tttgccgctc gagcgcgtct cacgcatttc ggctggcgtt cacaatacgt
     3361 gtgccatcgt ggcggcatcc tccgccgcgc ccgggcaagt gcattgctgg ggcagcgatc
     3421 aggacggggc tctcggagat cgagggtccg gggatttcgg tgacgatgag ccagtcgaca
     3481 atcgtcgtcc ggtggctctc ccggagaatg cactccagat tgcggtcggc ggcgaattcg
     3541 cgtgcgcgct gctcgtaacg ggagcgctgc attgttgggg cgacaactgg gccaagacgc
     3601 tcggaattgg ggacagtaca tgtgatatcg gggacgaacg accctgcatt ggagagttgg
     3661 ggcgctctcc gaatatcccg gtcggcaggc tgggaacccg gcccatcgtg aagctgagag
     3721 ccagtcagtc gtcggcctgt gtggtcgaca acaccggtgc cctacgttgc tgggggcgaa
     3781 actcccaaag ccggctgggc tatccggagc tggttcaagg cgagtttttg ggcgctccgc
     3841 ccgaacccgt cgagcttggg tctggcgtga cgatagtaga tgtagccttg ggattgcggc
     3901 acgcttgtgc actcgattcc atgggccagg ttcgctgctt cggagagcaa ggccctgcct
     3961 tgggctatgg catgctcggc gaagaagggg tcgctgggat cggcgggatt cagactcctg
     4021 cgagcgcata cgccctgcgt gaggaccgtg gaatcgtcga tttgggagat ctggacggag
     4081 ttgctggtag tgatcgcgct caggcgatct tcgcgggcgg gatgagcacc tgcgcacgca
     4141 tgctagacgg gggcgtacgc tgctggggag ccaactcggg tggtgagctc ggatatggcg
     4201 catacgacgc ggtcggcgcg atcggggacg actcctctcc cgcggatgag tatgtgcgtt
     4261 tgggcgtgcg cgcgaatatc ttcgccgaac cgtgatagtt ccgagttgcg cgagttgcgc
     4321 gagttgcgcg agttacccgc ctcgatgaac tcgatgacat gattctgtag gcagagcgcc
     4381 aatcgcaggt ctcgaaggct gggcgcggtt gtttccctgg ttttcgggtg gcttggcctc
     4441 gatgggtgcc gcgctggttg ctcagcagga agataggtcg agcgcggcac gccgagtgca
     4501 ttggcgggtg aagctgcgcg atccgcggac atgctgttga tcggatgcgt gcggggtgtg
     4561 tgaaccagaa gccaaaaggg aaaatcatgc tgctctgtca gaatagactc gggaaattca
     4621 agggactcag cttcgctcgt gctttctcct tgctatcgtt gccgctgtgc ttgggcgcgt
     4681 gtggggccgt ggatccgtca gcggcggcat ctctagagga tgatcaggcg gcaaccgcga
     4741 ccgaaagtgc ggcgttgacc gccgatgatg gatacgttca atccatcacg ctcagaatgg
     4801 tcggcggaga tccggttcag actgcctata cccacaacgg gagccgagtt cagctggaca
     4861 ccaccccgtc agccggtaag gcaactctgg atgcgccccc tgcgccgggc gcgcagcatc
     4921 gaggtaaagc cacactccgg gtcacttgcg taaatcccaa tggggttgcg atcgtgaaga
     4981 cccagggtgg tctctacaac ctggtggggg tgggcaaagc taccttcacg gcgccctgtg
     5041 acccgaacta tcgagccgcc aagagcgagc tcattctgaa cccctcggat gggatcgggg
     5101 acacggtgaa ttaccttcaa ttagcgccct caccgagcgc ggatcgggac ttttcgatcc
     5161 aaatcaaagt gccgggcgtg gccgccgcca ccagctctcg caacttcgtg aagtatggat
     5221 acctgacgct gaatactgct caagggagcg cctggtttag gtacttcaac gcttcgaacg
     5281 acgcacgaaa cgtgctcatg agctatcgaa ttcagtgcag cggaacctgg accgatacgg
     5341 tcacgcgcga cgcggtggtc gggccacaac tccccactaa cttcgtactc tcgtgtcccg
     5401 cgggcacggt gctggaacgg gtggataccc gcctccagaa cattcgctga gctacttcca
     5461 cgagtagccg tagagagccc cacccaacct ctcgcccgag tgtggcgttt caaagctcac
     5521 cagctctggc gggaggcttc gttgtctgcg ggatgctttt cggttgctcc gcaggtcgga
     5581 gccgttccga agtgcgggag tgctcgaggt cgtgatgccg ttcggagtgc atggttcgat
     5641 gaagtacttg attctgttct tgccgctagt gacactcggg cactgtaagg gacgtgacgc
     5701 tgacgagcct gcaatgcttc ctgacaccga gcgggtttca gccgcgtcct cgacgaagct
     5761 acttcaatct agcgcttctc cagtggcctt accggccgcg cccgctacgg ggttcgcgag
     5821 cgcgccgaaa gcaacagctg agccggggtt ttgcgaggtg atgtgcggcc atacgcgcgc
     5881 cctcgggtgc actgcgcttt ccgagtgttc ggcgagctgc acggccttgc tggcagatca
     5941 gcagtgtcac gaccagctga tccacgctct gagctgtatg atcgcggaac cgcgtaccca
     6001 ttgggtgtgc ggtgaggatg gcttggccgc gatcgattct ggatattgtg atgctgagca
     6061 gcgcgccttc gtaggctgtg tgcagcaggc gagctgaggc caggagctca gcattcgaga
     6121 accgggcgcg ctcggtgttc gcacgcgcca tggggaagtg ggctcgtgtt cgtgctcacg
     6181 cggtgaactg tggaggcgaa cgcccacgaa ttttcggctt tctgaagggt cacggtcatg
     6241 caatacgcat cttcgatttc taaaagacac ttgcgcatcg gattcgtggg tagcgcaagc
     6301 gctttcctgt tggcgatggc actctcgggt tgctcgagcg tcacaccgag ttcgacgtcc
     6361 gatcaggaga cggtctcggt cgccgagcag gcgctcacca cgtccttctc ggttataacc
     6421 ctgagcggta atccgaacga cacgatcctc tcggccacgg gcaagctgtc gattgattct
     6481 cgggtcgata ttggtgacgg ctccaagttg gagacagtga gctcctttgg ggcagggtcg
     6541 gcgaaccaga cgttcctcca atcggccgcg gtgatccatg gtaatctgac cagcgtcgcg
     6601 ccggtcaagt tggagtctca ggccaaggtg gaggggtttg cgcgcagcgc cgggacgatc
     6661 accaaagacg ccggcgcaac cattgccgtc gaatacccga gcgccaccaa catcactagc
     6721 cagaccacga gctggaacgt caacttccct gataccgatg ggggcgccgt gaccgtcaac
     6781 acgctcgagc agcgcacgct tgcgccgggg gcgtggggag acgtgcagct gattggcggg
     6841 gataccgtcg aacagggagc gaagctcacg ctcacctccg gcacctactt cttcaagtcc
     6901 ttcgtaagta accccaagac tcagatcttc ctgaacaagt ccgccggccc catctatatc
     6961 tacgtaaaag agaagttcac ctataagggt ggattcgtga cgaccgtagg ggtggcgacg
     7021 gagacgctcg tagcggcgct gggcacggat gcagccggta gcggcgaggc cgtggtgtat
     7081 ctggaggctc caatgaccgg aacggtggtg gctcccaacg gaaccatcga cctgcgacga
     7141 ccgcccaatg accaacctca tcgtggcgcg ttcttcggca agaacatcca cgtcttctcc
     7201 gacatcgacg tttaccacgc gcccttcgac ttccgttgct cactcggcga tcaagatcgg
     7261 gatggcaccg acgactgcac ggacgcctgc ccgctcgatc cgagcaaaac agcaccgggg
     7321 ctcgccgggt gtggcaagtc agagaccgac gcggacggcg acaagctgcc gaacgtgatc
     7381 gacggctgca agaacgatgc gaaaaaccag gtcgaaggtc agtgcggctg cgcgggttcg
     7441 agcgccgccc cggtcggaac cccctgcacg gacagtgttt gcccatcggc gacgaatacc
     7501 acctgtgatg gtgccggtgt ctgcggggac ccggcgagct gcgcgcccgc ggcgagcggc
     7561 tgtaccgtgc ggcgctttca gaacagcatc tattggtttt gcaccagccc agtcacctgg
     7621 tcgcaggctg ctagcaattg ccgcgcggtc tcgggtcgct atttggcgcg catcaacagc
     7681 cgcgtcgaga acgacttctt cgcgcaaagc ttgggcggga aggcctggca cgggggcaac
     7741 gatagctcga ccgagggcac ctggcgttgg agcagcgcga gcagcaacaa cggggacccg
     7801 ttctggtctg gagacggcac gggcagctcc acgagcgggc gttacgcaaa gtgggcacaa
     7861 ggccagccca cgacggccaa cgactgcggc gccgtacagc ctgcgagcag cggcggccaa
     7921 tggacggccg aagcctgcac cggggccctc gcttacgttt gcgagcagcc ggtcaacttc
     7981 gcgcctccgc cggttcggga tctcgatctt ggcaagttct tcccggagcg cggcggcagc
     8041 actccgcctc aacaggcgac gagctgcgtg cccgaggcta cggctctgcc ttggacgaaa
     8101 gcgcaggccg agcagcaaat cactgcctgt aatgcggcgt tcaacaatgg cacttgcacc
     8161 gagactcaga gcgctggttg cagcgctgca tgcaaaggga tcgccaccgt accgcccgct
     8221 ggttccacgg gatcgtgttc gaagtacact ccggacgagc tcgcccagtg cgcgctgcgg
     8281 aacgtcgtcg aggcgggctg caccgcgggg gtcgactgct gctcctatgt ggagaacgag
     8341 atattgagtc aatcgttcga gacgtcggtt ggcaccttca cctacgtcga cgatgccttc
     8401 ctcggtacta cgcaaccaag ctacgcggca ggccagcgcg tcacgagcgg cggcaacccg
     8461 ggcgcggcgg cgcaggtcac gctgggcggt atcgacaata ccttgatcac caacatgtcc
     8521 ggcggctggc ggagcaactt cacggtgccc gccgtgtcgg atgtaatcct gagcttcgac
     8581 tacaacctga cgcagtcgcc caactacgag tcgtcggaca tctctcaggt gatggtgcgt
     8641 gtcgacggcg tggtcaaggg cctcaacctc agggatcacg tcgagcagat tgcaggcaac
     8701 ggcgatggtg gggccaacat ctcgacgggc tggcgctccg cgcgcttgaa cttgggagac
     8761 ttgagcgccg ggccacacgt attctcgatt ggcgcctaca acaacgccaa gaacaatgcc
     8821 aatgagacaa ccaccctgct aatcgacaat gtccggttga tcgcgcgcga tgacacgtgc
     8881 gctgcgggcc agatttgtgg gcctgtctac tcttccgggt gcaatccgtg tgacacggtg
     8941 gacgcgaacg gcaagtgcac caagaattgc cccccgccgg tcctgcgttg cggcacacgc
     9001 acggcgaagt gcgctgacac cagcatctcg ggcagtacct gctcgatggt ggagctttgc
     9061 tccgggccgg aggcgacggg cagctccgac ccgaggacga atggagattt gacggcgagc
     9121 cccgcgttcg atccagtcac cgagtttggt cagcccacgg ctcccgcaac caagtactac
     9181 gccgatcccg cttgtgaaac tccgccctgc tccgcgggtt tcaataaccg ctggtgccgc
     9241 tacgaaccaa agatctggaa cgacccggtg gtgaagagaa tgaaccccga ggtgatcgac
     9301 cctatgcccg ccaaggcaac ccgtccggac acaaaacggg gcgaatcggg gagctcggcc
     9361 ctgaaattcg tgttcgatcc ggacgcccac ctcgactact cactcgatcc tctcggcggc
     9421 ggacagacca acttctacct ggatacgtcg gcgtcgttgt tgtccacggt tggcttcgac
     9481 atcggctccg acccactgcg ggtcaaagcc aaggtcgaca tcgtggacgc cctggtgaag
     9541 ctgcacctcg accgttgtca cgcgacgacc ctcgagtcca agctggtgct gttcggtgac
     9601 gacttcctgc cgtccttggc accgcagttg ttgttcgacg agcagatccc gaactgcgag
     9661 gccgcgatcg acgaatacgg caaggccctc aatcgcgtca ggaaggccta caaggacgcg
     9721 atggagctcg tgcgtcagta caaggctcgc aagtccgcgg ccgagaagtt ccccgcggac
     9781 ttctgccaga agctcaccgc ggagctgccc gacttctttc cggccggatc gtgcgcgacg
     9841 gagacggccg cggccacagt caatcgcttc atcgactact acgagagcga gatcaagcga
     9901 gtgctcgagc ctgccaacgt gcagttgacc aacaaggttc tgagtgtgtc tggcactccc
     9961 atcaatgtgc cttccactcc aacgcgggaa tcagagactc tggtcgaggt gaccttcatg
    10021 ctcggtccgg tcccgatgcg actcgaggtc gaggccttcg tcgagtacgg aatcggcggc
    10081 aatcttcggt tcgaactcaa tccgaacgcg ctgctcgcga acacgggcga gcgggagacc
    10141 ctggcgaacg tcgatgccca cgtggatccc tacgctctct ccggcatcac gatgttcgtc
    10201 ggcgcagggt tcggctgggg tgacttcggc gtctccgccg gtgtggaggg tgccgtgacc
    10261 ctcggtcgac tctctctcga caactacgcg ggtgcaggcg tcgccgtgca agtcaccaaa
    10321 gatgatcggg agttcccgtc cgacgtcaag gcggcggtgg atcaggccac tgcggatggg
    10381 cggctcacgt tgttcccgcc cgcgggcgcc aagaagtacg acttctccct gcagtacaac
    10441 tacggatcga agattgtcat cagcgaggtg ctaaaaggcg cgattgctgg tcgcgtgcgc
    10501 ctgaagatgg ccttcttcag caaggaatgg cgtaaggtgt tgttcacgtt ccccggcttc
    10561 ggtcccttcg aagtgccgtt gatctcgggc ggaggcaacg cgcccgcgtt cagcatcgac
    10621 ccgcataagt ggggcagctt ccaggtgccg ttcccgttcg ctaagctcgc tcgcctgaac
    10681 ccactcgaag tgctcaccgg aacggctcgt gcgttcgata ccagcaacgt ggaggagccc
    10741 ttctacgaca acctgtgcca ctgctcgaac accgctgagc cgtgcacgcg gcgagccgat
    10801 tgctgcgatt cggccaatca aatttgcttc tctgatccgg cggttggcgg gcaaggcgtt
    10861 tgcagtgcct gtcggcagcg agctcagact tgcaactcgg tcgctgattg ttgcccggga
    10921 cgtggggtga cctgttcagc accgctcgga tccacgacga aggtctgtgg ggtcactgcg
    10981 ttctgatgtg attcggatgg ctccagcgtg aactgccgag ggtttgtgat gcaatccaag
    11041 attggtgttc gaggttcgct gcgttcgacg gtttttcgag tcgtcctggt cagtcttcca
    11101 ctcctggccg ccagcggcgc gggctgcagt ggagattcga agacggcgga ccgcggagag
    11161 acgggtacgc ctctgcaaac agagctcact gccaattatg ctcgggcctc ggtctacgag
    11221 gtggagctag aagcggaaag tgaagtcggg cccgggcaag cgctgcagct tcgggtagtg
    11281 ggcaagctcg aagctcatcc gattccagca gcgcggggcc agcaggggct ggtccttcga
    11341 atgcgagacg cacgagcctt ctccggcgcg ggggcgaccc aggagatcca agagctatct
    11401 cgcgagatcg cgcgcgcgtt cttcgttacc atggagaagg ggcagactcg ggagctcaga
    11461 ctcgcccggg gcatgagtgc ggccgcggcg agcgtgcagc gcacgctggc ggcggcactg
    11521 cagttctcca agccgagtgg cgggaacagt tggaaaggcg acgagttcga cgcgacaggg
    11581 aagtacgagg tggcttacga gctcagccgg gccggcgcgg gagcaagcgc gtcggcccac
    11641 gtcttctcga aaaagaagct gggttatcgc gcgctctccg ccaacaggac ccaattgcct
    11701 gcccacgttc aggtgcagtc ggcccaagta tcggcccagc ccgatccgtc acggtcgttg
    11761 aacctgcacg tcgacgttcg cgaatccgac ggtgaggtgg cgctcggccc gaacttagaa
    11821 atcgagcggg tgcactatcg agagcgtctc gaagcgaagt tgtttcaggg cgctccggtg
    11881 gcgtcgaaga cgaccttgac cctcactcga ttgaccggag cgtcaccgac cgcggcaccc
    11941 aaggagacgg agagcgtgag cgggctcgtc accctgcgcg gagacgcgcc ctatccggcc
    12001 gcggcgcccg tcgacttgga tcgcgcgcgg acttcggggt tgaccttcga gcaggtcgtt
    12061 gccgagctgg agacagcgga gcaaggttgg gcgcctccgg acaacgggcg ccctacggag
    12121 aaggagagcc ccgccgatgc agccgcgcgc gaccgggcgc agcggctgtc gaagcacttc
    12181 gtgtctctcg aagccatgtt tcgacgacaa ccagccacgg tgccgctcgc gatcggcaaa
    12241 attctggcta agtctccggc ggccgcggtg ataacgaatg cgctcggctc ttcgggggca
    12301 ccatccgcgc agccagcgct gctcgggctg gccaagaatc gcagtgtgga agacaaggtg
    12361 cgagccatcg cggccaacag cctgatccgt ctagcccggc caagtgagga gaccgtaacg
    12421 gcgctgatga cggtggtgga cgatcccgtg gtgggcgttc acgcggtcta cgcgctcggg
    12481 agcttcgcga ggaagctccg cgaggacgag ccgcagctcg cagagcgcac ggcacggttt
    12541 ctagtgtccc ggctgagcgc agtaaaggac agcgcccaga agatccacgt actgcgggga
    12601 atcgcaaact ccgcgtacgt gggtgcgatc cccgccgtac gtgaagtgct gtcgagcaag
    12661 gatgccttgt tacgatcagc ggcggtggag tcgttgcgcc taatgcaggg acccgaggtg
    12721 gacgcgctca tcgcggatcg gatccaggac gagcgcaagt ccgtacgcaa cgcggccctg
    12781 agcaccgctc gagtgcgcgc cccgtcgaac gagctcatcc gcgccgtcga aggcgtggcc
    12841 atccgcgcgg acgacggcta cggacgaatg gaagcggtgc gcttgatggg tagctggctg
    12901 ccgcgagctc cagccctgcg cgaagccctg ctgcgtattg cccagcgaga cgaggaaccg
    12961 cgcatccgag agaccgctca gaagctaatc gatggttagc ccgagaatcc aagtaactca
    13021 aaattggtat tcgagtctga actgacccgg tgatgcagcg cgcagctgaa cgggcttgga
    13081 accgggactg ggggccaacg ttagataggc gccaacccct agggctaggg cgccagaagt
    13141 gagcattgca acatcgaccg cgtcgacgag ccgaatggac gacaggaggt cgtcattggc
    13201 gctggcccgc tgccgcagac tgggatccaa actcggaatt tgcgacattt ccaaggcacg
    13261 gttccgatcc gcttcgaggg cggcattctc cttcgaccag ctgt
//
LOCUS       DASTKU010000277        17012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1169494, whole genome shotgun sequence.
ACCESSION   DASTKU010000277 DASTKU010000000
VERSION     DASTKU010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 17012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17012
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169494"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..866)
                     /locus_tag="VFQ61_09965"
     CDS             complement(132..866)
                     /locus_tag="VFQ61_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HET9954821.1"
                     /translation="MGLLDIMRPEHSLGFEAGSAQDGARLAAVLALGVCTAVRQKAIT
                     PRYACEKLFRPSLEQTLRQLGVDSKLCDSIAQALELEDVSNLDPEALERSLDGLVTAL
                     TDFLAKEPDVELPERESWLRVDEPSQRFREGTFLDRLQLTWKQLQLNGASGIARVCGV
                     YEITDQHRIPGGKFKIKVLERESDFIALPNVCIRSPDGVPEWSCGLGPSELEALQDAV
                     TRVTSDLSARDEWSDESLEWSDPRDF"
     gene            complement(913..1962)
                     /locus_tag="VFQ61_09970"
     CDS             complement(913..1962)
                     /locus_tag="VFQ61_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954822.1"
                     /translation="MASPESGGPAVRFIVSCAALVACNALLGNEQPEKLAKGSGGQGG
                     KATTGQGGTAGRGGDPQTEGGASEGGSSADSSGGMSNAGEAGRGPSDAGGTAQVLFES
                     EFAKLDGWVAFQKTQFDADGGWKAGSTNRPTPDAVCPQPALDPWQVVSGLLQYSTYCY
                     LTGTRFPDQATQDRLVSPEFSGSDADFKATLSFKTSDNDVVGLVFRHADENNWAFFTT
                     LWDASWSSGEPGLRHALVSVKDGQFETLGRSISSVGYDCAPDCSACQPSGSAGAAGAE
                     AIYPGCTEAWQTLDVELSAGTLRAFVNGLLALEVTDAAGRIPARGSVGILTSSMNNGT
                     PPLRVQSLRVLGGVK"
     gene            2104..3171
                     /locus_tag="VFQ61_09975"
     CDS             2104..3171
                     /locus_tag="VFQ61_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954823.1"
                     /translation="MRSDYRSPSLSSALTLLLAASVLLHCGVTEKSAPTPNPATQGGS
                     GGRSSGTAEGGDDARAGEGGEAGVANGGASGVGGGASGSSGTLAPQGGTTLQTGDGGS
                     AGAIRGEGGEGNGQGGSEAGAGGAAGESTAPISGGRSGGSGGQSNASGGGGQTSASGG
                     RTNTGGGGTTAECSLNVHCSNASRPFCSPQGRCVQCLNEGQCPAPTAECLKRACSNYT
                     CTEVPQTANTACGDRASTTCSAPDTCDGRGNCIANHAAPNTACDDGQYCNGADRCNST
                     GVCEHAGSPCTAPLVCYPATNSCRCVNAGRSVADYCYSDPSQIEGLDRPATDTATAAC
                     GACGGFNRVSSGNCVDFVCKG"
     gene            complement(3230..4744)
                     /locus_tag="VFQ61_09980"
     CDS             complement(3230..4744)
                     /locus_tag="VFQ61_09980"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HET9954824.1"
                     /translation="MRWMRLGWTLGALVAGLVIAGPAVAQDLGLVTTLVHPNMRESKE
                     DRFGSGVAISGGTLFVGAKDEGGANDYSGAVHVFSDQDWSEVALLKSSNDRDIEFGAE
                     VQLDGDRALISNRAIDNGVVWFFERVGGVWTEKLRVQGVVNEAFGYSLALRGDFAFIA
                     ASRVGTGSVHVYRRMAETWSEVQVLTASDAQDGDFFGYAISFDGQRLAVSAPGNLLGP
                     KRCGVYTFSRQGDGFVEDGKLAGDPATQWFGADVGVSGEMLIVNAPPAYQVTGVGKAL
                     VYQRRGEEWELDSELTMEDDPSFPGALTLDGDKLWFGTRQSEPKGLNVYAFERGSEGW
                     QRGQMLTFPTPGMYELASWAVQDDTLVVGFWNGLRTESVQVYRGAGHQSSSGGGGASG
                     SGGAVSSGSGGAGIVAPGGTNSGGTTGENTAAMAGTNSSVSGGNGSIEPSSSGGASSR
                     GGETTTAQPANSESSDDGGCSVVTGRNSRAWPVAALALAVALVGRRRNRRAQLR"
     gene            complement(5001..5900)
                     /locus_tag="VFQ61_09985"
     CDS             complement(5001..5900)
                     /locus_tag="VFQ61_09985"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9954825.1"
                     /translation="MNTSFNSFARAFAAATLAIALLPGTAQATEGILQSTGSMTLTED
                     HFGTIFLFQANGTLDCQNHTIYNSTQEGICGDNFDEQCGVLVLASPDVHIVNCRVRYF
                     GIGMHVQSSNNVMIQSSDIRENFQGIRMRWVNNNAGHFFRDNYVGFNNEEGADIDDSS
                     GLQFLGNSQFRYNGRDGLDCGNSTVKILSGNAFRNKFNGYELDGCNGSILSNVLAQSN
                     GNTGISGEVRSGISLDATQNFVISNSVVNSNYRDGIRVANGSNNGRIEANSGAGNGEH
                     DAHQTSSVGNTWIGNVFGTTAGF"
     gene            complement(5918..6505)
                     /locus_tag="VFQ61_09990"
     CDS             complement(5918..6505)
                     /locus_tag="VFQ61_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954826.1"
                     /translation="MTIANKALFLLLLAGCANVPEPDESLAAHSSSATPARVADVARP
                     TVNPRVTASRQRFAESLDRYRRELVARMELSASDTAELLAILKEHDDARMRMVAELDT
                     NPGQTEQALALRADFAAVDPAAVEERISVLLGNDKYRKLTQLRETREQWLPAISPRQA
                     GGGPALRRIGEARLQLPSGPLKSEARSLHDAVDSH"
     gene            complement(6601..10023)
                     /locus_tag="VFQ61_09995"
     CDS             complement(6601..10023)
                     /locus_tag="VFQ61_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9954827.1"
                     /translation="MLTNLRSLRYRVPHGWTIACVLIGPWLSGCEGGSSPSLSSPVRA
                     SDPAFHASQAITSCGSRTTQTWGAGSFFTTAGDFFETGSRDRIWVTDANGDGVGDVIG
                     IGFDGTVWVATGTNGQYNSAVLFGATRFTTTARWFTNDYRQRVFVTDVTNDGIADIVG
                     VNDAGTVYFYLGTGTGFVQKTARSTVFAAATPNVGDGFWDAADSANAYAGTRVWLADV
                     SGDHKLDLVGVSNAGDIWVYPGTTNGFGAVAGPFPSAFKNSDGYFQTRDSNGLLAYHD
                     NVWVSDYTGDGVSDIVGIAPSGQVQLSRSLGNGTTVSFDSTITLGPTVFRRNAGWFDV
                     GSQPRIWLADVSGDGRSDVVGIAPPGQGDGDIWVAQSSPWSLVSKVWLHGSVLKTQND
                     WLSNAYHPRIWVGDVSGDKRADVVGVDRAGGVWVAKSLVEESDTSGTTSGSFLPSLRV
                     GVSDLGESGQVPGGYFDKAFKRRVWLADATNDGGLKDLIAIRPNANDGDITWTSVLPT
                     SVVSIADITRDQVRSGGVIPVQVKFSRAIDCAATPANALVVWEKFGSTNRKIISQSRT
                     QVDPRTCAFDVALLAAGDADVKLTKSVEGIGLGDRFGNTVDGNGNGFIDCARDKNERI
                     DYIYSSLVGGADKTSLDYADGPAPLFPSADLLPVPFKRFPNSPPPMARFILLGGGSRN
                     VAVLMSVELVGVNPGRLRDLISARTGIPTRNVVIAATHSHTVPHTLNLYTAPTYDDRS
                     FTGPAPALYEYPAQEYSYVQWIEDSIAEKVAATYASLVPVKVAVGTTTFEATNDAGPL
                     INRRDNAWQSAGSVIKQQCPASDQFLDPRIGMIAVYTASQAPLAMLVNFGLHPVIVPS
                     SLGMTPDFTGYMSQCLEDGCAGISASLPKFPTSLFVQAGGGDIIPTGTWTQTEADADR
                     ISRGYGRQIANRVLSKLPQLAISTTNGMSVKAKRTPHMFRGRGSCNQTTNCPGNESRP
                     DEMEVDWQPESTAMVIGAPNNTLLALGTVPGEAFSTLNPRTYANVTSAVGSGKTFLFG
                     YANGYFGYFPDTEARSCALALEQHNPADACSGEGPAYYCDANGDLQVPPVPGPRVVPG
                     ELCYAVFQCSDDAPLLYGTGPTFLGEEWTGAKTAGESIVDWNVSVLNQLATQ"
     gene            complement(10215..10547)
                     /locus_tag="VFQ61_10000"
     CDS             complement(10215..10547)
                     /locus_tag="VFQ61_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954828.1"
                     /translation="MAVPAAMRGQLVDEVGLGSSVSTSLVVDHEPERRTSTAWMFEAG
                     TDSGVFLPSLALGAGPLLRLGSGKAAPGARLQVALSFKLLSLGSSVDIHPGAKEELAS
                     FLWGQLSL"
     gene            complement(10754..11473)
                     /locus_tag="VFQ61_10005"
     CDS             complement(10754..11473)
                     /locus_tag="VFQ61_10005"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9954829.1"
                     /translation="MSTEPTSSKPPQGVPVRIAYEAWAAGYDDDENVTRDLDAEVLRS
                     AGLPLRGASVVEIGAGTGKNTAFLAEQASEVIALDLTPPMLERARQRCPAPHVRFIEH
                     DIREPWPVSTGRANVVVGNLVLEHIENLTPIFSEAARVLSPQGSLYLCELHPYRQLRG
                     TTARFQNDGESVRVEAYLHMTADYVMAARAAGFELVSMAEPNELGSPELALPRLLQLT
                     WVKAQRADTTEPSPGDSRLHR"
     gene            11667..13418
                     /locus_tag="VFQ61_10010"
     CDS             11667..13418
                     /locus_tag="VFQ61_10010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HET9954830.1"
                     /translation="MNPDAVPNLHRVASREASLAGGRGPLALGAWLVALLSSSAPAAQ
                     AKSFIDYLEPTPITCSPLSSASWGVAGVLPRDLCNGIESAKGAGVPPDYYYWDGQIIR
                     TKDGKYHMFMSTWAGSAGFNPGWTGSDAYHAISEQGVLGPYKRQGFVYTNNGSHKGHN
                     VTALELPDGSYAVVVSEIVPFTIYKSNSLDGPWTSCTASIQTNGVNAGTDSHYDSNVS
                     LTARHDGKFEIVQRHGLIAIADTLCGPYKMQKPTWAYPAANRPSIDSIYPRRTSIPDP
                     SITNPTYTWEEDPHIWISGGVYHVLYSGSGDRIGYHLYSPDGINNWKDGGLAFNPRMA
                     EKIFCYQDSTTCNKWYKMERPGVVLENGRVTHVTWAVADVDKDNQIPAGSNHGSKVIV
                     VPFDGVAFDADYGVNGNAGAGGAAAMGGSTSAGGRAGGGGLPGSGGTTSAGAGSGGTT
                     SSAGAQTGLGGAGPGGTGLGGTGLGGTGLGGTTGASGSAGLSSTGGSTDRVASGGKTN
                     AAAGGSTGTSEANGGLTASGGSVTAGNSGNSASSVAKDSGCSCSIPSNSPSGAWGSGL
                     LLGGLGTLLVKRRRQRR"
     gene            13620..15518
                     /locus_tag="VFQ61_10015"
     CDS             13620..15518
                     /locus_tag="VFQ61_10015"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9954831.1"
                     /translation="MKPPRNYRQCATSDGRATRIATTASLRAVLLLVLGCSSGAVTEA
                     PESGSLGGGGPQGNSGAGGAAANAGQGAVAQGANGQGASGQGAMPIGGASAAGATNTG
                     SAGQKDDPYAIPSSPPNSVVVPTPRVARLSRLQWSNAVRDLLKLADISEVDSGVTGDA
                     LIGFDNEAESLFVTEQLRQQLATAAEKLASKVTSDASALARLLPANAPSSGSARARAF
                     VTTFGQRAFRRPLTDAEVTTHLGLFDQGPTLYPGVDAFAAGASLVIQAMLQSPHFLYR
                     TELGTAAAGAATVALSDWEVASKLAFAITNTMPDDALLSAAAAGQLRDRASISAQAQR
                     LLEGATGTAGLRNFNLQVYRLGTYDGIERDATRFPDFKVNTPAAMKAEVLQFIDWLFT
                     QGRGIKDFYTTPVGFVNSLLAPLYGVSGNFSSEPAALSKVDLDPTKRSGLLTQAGFLS
                     SYISVGDEPDIIHRGVFIATRLLCKTLPPPDPAAAGKGLVDTPNLTNRERVEATTGKG
                     TCGAVCHGALFNPLGYAFENYDAVGKYRTQDQGKPVDAADSYMLDGQLKSFKNGVELS
                     ALLAEAKETHSCYLQNMLSYLQGQELSEQQRPLVDYYARLSRAGKVSLRDLELQIVTS
                     EAFLNRRP"
     gene            15554..17011
                     /locus_tag="VFQ61_10020"
     CDS             15554..17011
                     /locus_tag="VFQ61_10020"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9954832.1"
                     /translation="MNRRLVLRGACGLMLGLPLLETFMPRRAEAQTSTRSPFVLIVVG
                     DNGVVQAGVTLSGGGEPEKFWPTQTGALTKDKLLADKASRSIGELADYAEKLLIVRGI
                     NLPYNSTGCSHSAADAQILTAAKITAGSTNCKALGISVDTAIAKAKNPPGRDPLVLHA
                     GMFSPGGTGFDIPGYVSYITPQQPRVYIDSPYKAYQQIIGAVGNGTTGSSTDDPAQAL
                     RAARSKSINDILRPQIRALLARQDLSQSDRDRLDQHLTAIRDIEVKVSMTTAFTIPDA
                     DVATMKSMDPKPYDQSTRDAAVKLFMELMAFAAASDYTRVAVLKVGDRIDDHVYTYNG
                     KSAKFHDVSHRQVANAVDFHHYIDRMMLNQYKYLLDTLSKYNTPSGPLIDQGVAIWTN
                     QCATGAHSFSNIPWIQAGTANGFFKQGEYLVVGNGQQPGGSQDGKGGDGSVSGINKML
                     NTVLTAAGVTKTGGAPTDDFGEPSLPKGIFNSMLA"
BASE COUNT         3330 a         5475 c         5115 g         3092 t
ORIGIN      
        1 catcgacacc gcaagcgcga gcgttcaata atgctctcct gacacaaccg ttcgagtcag
       61 gatcgaatgt taaagggcac gagcgggggc gagtcctctg ctcccgttcg tgtccgcgtc
      121 ggcgaggcac atcagaagtc ccgggggtcg ctccactcca gtgattcgtc cgaccactcg
      181 tctctcgcgc tcaggtcaga cgtgactcgc gtcaccgcgt cctggagcgc ctccagttcg
      241 cttgggccca atccacaact ccactccggc acgccgtccg gggagcggat gcagacgttg
      301 ggtagcgcga tgaagtccga ttcgcgttct aacaccttga tcttgaactt accgcccgga
      361 atacgatgct ggtcggtaat ctcgtagacg ccgcacacgc gagcgatccc ggaggccccg
      421 ttcaattgga gttgcttcca ggtcaattgc agccggtcga ggaacgtgcc ctcccggaaa
      481 cgttgactcg gctcgtcgac gcgcagccag ctctcgcgct cagggagctc gacgtcgggt
      541 tccttcgcga gaaaatccgt caacgccgtc acgagcccgt ctaacgagcg ctcgagcgcc
      601 tcgggatcca agttggagac gtcttcgagc tccaaagcct gggcgatcga gtcacagagt
      661 ttcgaatcga cgccgagctg acgcagcgtt tgctcgagtg aaggccgaaa gagcttttcg
      721 caagcgtagc gtggtgtaat cgccttttgc cgaacggcgg tacacacgcc caatgccaac
      781 acggccgcca agcgcgcccc gtcctgcgct gaacccgcct cgaacccaag ggagtgctcg
      841 ggacgcatga tgtctaaaag tcccattcag aagtcctgtg gctcattcag ccctgcggtt
      901 cctgggttgc ctttacttca cccctcctaa tacacgtagt gattgaactc gcaaaggcgg
      961 cgtgccgttg ttcatgctgc tcgtcaagat ccccacactc cctcgcgccg gaatgcggcc
     1021 ggcagcgtca gtgacttcga gcgcgagcaa accattgacg aacgctcgca gggttccagc
     1081 tgacagctct acatccagag tctgccaggc ctcagtgcac cccgggtaaa tggcttcggc
     1141 ccccgcggcg ccagcgctgc cgcttggctg gcaggcgctg caatctggag cgcagtcgta
     1201 tcccaccgag ctgatgcttc ggcccaaggt ctcgaattgt ccatccttga cgctcaccaa
     1261 tgcatgccgc agtccgggtt ctccgctcga ccaactcgcg tcccacagcg tcgtaaagaa
     1321 tgcccaattg ttctcgtccg cgtgacgaaa aaccaagccc acgacatcat tgtcggatgt
     1381 cttgaaactg agagtggctt tgaagtccgc gtcagagccg gaaaactccg gtgaaacgag
     1441 ccgatcctgg gtcgcctgat cggggaagcg tgtccccgtc aggtagcagt aggtgctgta
     1501 ttgaagtagt cctgagacca cctgccaggg atccagagcc ggctggggac acaccgcatc
     1561 gggtgtcggt cggtttgtgc tacccgcttt ccagccacca tctgcgtcga attgtgtctt
     1621 ctggaaggca acccaaccgt cgagtttggc gaactcggac tcgaacaaga cctgcgctgt
     1681 tccgcccgcg tcggaggggc ctctgcccgc ctcacccgcg ttggacatgc ccccgctcga
     1741 gtccgcggag cttccaccct ccgacgctcc gccttccgtt tgagggtcgc ccccgcgtcc
     1801 ggcggtgccc ccctgccctg tggtggcttt accgccctgc ccgccgctac ctttagctaa
     1861 cttttcaggt tgctcgttgc cgagcagggc attgcacgcc accagcgccg cgcagctcac
     1921 gatgaaccgg actgcgggtc ctcctgactc agggctggcc atggccagag actgcgacta
     1981 gcgaccagag gtgtcaaacg gctagcgagt gcttcgcgag accccgcgct tcgctcccgc
     2041 acggcctctt ttgcagctag tccccatcgt cgcaattcga cgggctgcta tggttgctgg
     2101 cccatgcgtt ccgactatcg ctcgccatcg ctctcgagtg ccctcactct gctgctcgcc
     2161 gcgtccgtgt tgctccactg tggagttacc gagaagtcgg cgccaacacc caatccggct
     2221 acgcaaggcg ggagcggggg tcgctcatcg gggaccgccg agggcggtga cgacgcgcga
     2281 gcgggagaag ggggagaagc gggagtagcg aacggcggag cctctggggt gggcggcggc
     2341 gcgagcggtt cgagcggcac gctggcccct cagggcggaa cgacgcttca gaccggagac
     2401 gggggcagcg ctggagcaat caggggcgaa gggggcgagg gcaacggaca aggcggctcc
     2461 gaggctgggg caggcggcgc cgcaggcgag tccacagctc ccatcagcgg cggtcgaagc
     2521 ggcggaagcg gcgggcaaag caacgcgagc ggcggcggtg gacagaccag cgctagcggc
     2581 ggtcgaacga ataccggcgg aggcggaacg accgccgagt gctctctcaa tgtgcactgc
     2641 tcgaatgcaa gtcggccgtt ttgcagcccg caggggcgct gtgttcagtg cttgaacgaa
     2701 ggccagtgcc ccgcgcccac cgcggagtgc ctcaaacgag cgtgttcgaa ctacacatgt
     2761 acagaagttc cccaaactgc aaacaccgcc tgcggcgatc gagcgagcac cacgtgcagc
     2821 gcaccggaca cctgcgacgg tagagggaac tgcatcgcca atcatgcggc cccgaacacg
     2881 gcgtgtgacg acggccagta ctgcaacggc gccgatcgct gcaactctac cggcgtgtgc
     2941 gaacacgcgg gcagcccgtg tacggcgcct ttggtctgct accccgccac caactcttgc
     3001 cgctgcgtca acgccgggcg ctcggtcgcc gactattgct acagcgaccc aagccagatt
     3061 gaaggtttgg accgccccgc cacggacacc gcgacggcag cgtgcggggc ctgcggtggt
     3121 ttcaaccgtg tgagcagcgg caactgcgtg gatttcgtgt gcaagggatg aacgaggagc
     3181 cacggagaga atgaactctc tggacgcgag cagcccgagg cgcaacgctt caccgaagct
     3241 gagcgcgccg attgcgacgt cgacctacta gagcgaccgc gagagcgagc gccgcgacgg
     3301 gccaggcgcg tgagttgcga cccgtgacga cgctgcagcc gccgtcatcg gagctctcgc
     3361 tgttggcagg ttgtgctgtc gtcgtttcgc cacctctgga cgacgcgcct ccggagctcg
     3421 agggctcaat ggatccattg ccgccgctaa cactcgaatt ggttcccgcc atggccgcgg
     3481 tgttctcgcc ggttgttccg ccggagttcg tgccgcccgg cgcaacgatt ccggcgccac
     3541 cgctcccgct cgaaaccgcg ccaccgctcc cgctcgcacc gccaccacca ctcgagctct
     3601 ggtggccagc acctctatac acctggacgg actcggtccg gagaccattc caaaaaccaa
     3661 ctaccaacgt gtcatcctga acggcccaac tcgccaattc gtacataccc ggcgtaggga
     3721 aagtcagcat ttggcctctt tgccaaccct cgctcccacg ctcgaacgca tagacattca
     3781 gacctttggg ttcgctctgg cgcgtaccga accacagctt gtcgccgtcc aaggtgagtg
     3841 cgcctggaaa cgacggatcg tcctccatgg tgagctcact atcgagctcc cattcttcac
     3901 cgcgacgctg gtagacgagc gccttgccga cacccgtcac ctgatatgcc ggtggagcgt
     3961 tcacgatcag catttcgccg ctcactccga cgtcggcacc gaaccactga gttgccggat
     4021 ccccggcgag cttcccgtcc tccacgaacc catccccttg ccgggagaac gtgtagacac
     4081 cgcaacgctt cggcccaagc aggtttccgg gagccgagac cgccaagcgt tgtccgtcga
     4141 aggatatcgc ataaccgaag aagtccccgt cttgtgcgtc actcgcggtg agcacctgaa
     4201 cttcgctcca cgtctccgcc atgcgtcgat acacgtgcac gctacccgtc cccacccgtg
     4261 acgccgcaat gaacgcgaag tccccgcgca gcgcaaggct atagccgaag gcctcgttga
     4321 ccacaccttg gacgcgaagc ttctccgtcc aaaccccacc cacccgctcg aaaaaccaga
     4381 ccacaccatt gtcgatggcc cgattcgaga tcaacgcgcg atccccgtcg agctggacct
     4441 cggcaccaaa ttcgatatct ctgtcattcg agctttttag taacgccacc tccgaccagt
     4501 cctggtcgga gaagacatga accgcgccgc tgtagtcatt ggctcccccc tcgtccttcg
     4561 cacccacgaa cagcgtgccg ccgctgatcg cgaccccgct cccgaagcga tcctctttgc
     4621 tctctcgcat gttgggatga accaacgtcg tcaccaaccc gaggtcttgt gcaaccgctg
     4681 gccccgcgat gaccaagccc gcaaccagcg cacccaaagt ccaacccaac ctcatccaac
     4741 gcatgaccgg aagttacccg aacccgtcgc acaggactag tccgtaccga aaagcagcca
     4801 atttgtcgga ttcgaaagtc accgcacttc gaagagttgt tgccccccgc gtcttccggc
     4861 gcgacacggc ccgcgtcctc gggctccgcc ctgcgggcgc gggcgccccc aagcgtcggc
     4921 tccgccgccg cgggggcgcc cctctgtaac gccacgctcg gtcctcgtcg atgccggtca
     4981 cacgacgccg ccgagtgaac tcagaatccc gcggtcgtac cgaacacgtt ccctatccaa
     5041 gtattaccga cgctgctcgt ctgatgagcg tcatgttctc cgttgccagc accggagttg
     5101 gcttcgatgc gtccattatt cgacccattc gcaacccgga tcccgtcgcg atagttgctg
     5161 ttgaccacgc tgttggagat cacgaaattt tgggttgcgt cgagactgat gccgctccgc
     5221 acctcgccgg atatgccagt gttgccattc gattgcgcca agacattgct gaggatcgag
     5281 ccattgcagc cgtccaactc gtagccgttg aatttgttcc tgaacgcgtt accggacaga
     5341 atcttcaccg tgctattgcc gcagtccaat ccatctcgac cattgtagcg gaactgactg
     5401 ttgcctagaa actggaggcc gctcgagtcg tcgatatctg caccctcttc gttgttgaag
     5461 cccacgtagt tgtcgcgaaa gaagtgcccc gcattgttgt tgacccaacg cattctgatg
     5521 ccttggaagt tttcgcgaat gtccgagctc tggatcatca cgttgttcga gctctgcacg
     5581 tgcatcccaa ttccgaagta tcgcacccgg caattgacga tgtgaacgtc tggcgacgcc
     5641 aggaccaata ccccacactg ctcatcgaag ttgtcgccgc agatcccttc ctgggtcgaa
     5701 ttgtagatgg tgtggttttg gcaatccaac gttccgttcg cctggaacag aaagatggtt
     5761 ccaaagtgat cctcggttaa ggtcatcgat ccggtcgact gcaggattcc ttccgtggcc
     5821 tgggccgttc cgggcaaaag cgcgatcgcc agcgtcgcag ccgcgaaggc gcgagcaaag
     5881 gaattgaacg atgtattcat ctcattctcc tcgtgcatca gtgggaatca accgcatcat
     5941 gcagacttcg agcttccgat ttcagtggcc cggacgggag ctgcaagcgg gcctctccaa
     6001 ttcgtcgaag cgcgggtccg ccgccggcct ggcgcgggga gatagccggc agccattgtt
     6061 cacgtgtttc gcggagttgc gtcagcttcc ggtacttgtc attgccgagg agcacggaaa
     6121 tacgctcctc gaccgcggcc ggatccaccg ccgcgaagtc tgcacgaagg gccagcgcct
     6181 gctccgtttg cccgggattc gtgtcaagct cggcgaccat gcgcatccgc gcgtcgtcgt
     6241 gttctttcag aatcgcgagc aactccgccg tgtccgaggc agatagctcc atgcgcgcga
     6301 cgagctcgcg ccgatagcgg tccaggcttt ccgcgaaacg ctgccgcgac gccgtgactc
     6361 gggggttcac ggttgggcgc gccacgtcgg caacccgtgc tggcgtcgcg gatgaggagt
     6421 gggcggcgag tgactcatca ggctccggca cattcgcgca cccggcgagc aagagtagga
     6481 acaaggcctt attagcaatc gtcatcgata cctccaccaa aacagaactc acggacgaac
     6541 cagagaacga cgcggcgaaa gggcgagcag gtctcgctct ccgccgcgtc gcggctccat
     6601 tcactgcgtt gctagctgat tgaggactga cacgttccag tccacgatcg actcacccgc
     6661 ggtcttggct cccgtccact cctcgccgag gaacgtcggc cccgtcccgt aaagcaaggg
     6721 agcgtcgtcc gagcactgaa agacggcata acagagctcc cccgggacca cacgcggccc
     6781 tggaacaggg gggacctgca ggtcgccatt ggcgtcgcaa tagtacgcgg gcccctcgcc
     6841 actgcaggcg tcagccgggt tgtgttgctc gagagccaga gcgcacgatc tggcctcggt
     6901 atctgggaag tagccaaagt agccgttggc gtaaccgaag aggaaggtct tgccggagcc
     6961 cacggcactc gtcacgttgg cgtaggtgcg cgggttcagc gtcgagaacg cttctcccgg
     7021 aaccgttccg agggcaagca aggtattgtt cggcgccccg atgaccatag ccgtcgactc
     7081 gggctgccaa tccacttcca tttcgtcggg acggctttca tttccggggc aattggtcgt
     7141 ttgattgcag ctgcctcgac cccggaacat gtggggggtt cgcttggcct tcacgctcat
     7201 cccgttggtg gtggagatcg ctagttgggg caatttcgac aggacccggt tcgcgatttg
     7261 ccggccgtaa ccgcgcgaga tgcgatccgc gtcggcttcc gtctgggtcc acgtgccagt
     7321 tgggatgatg tcaccgccgc cggcttgtac gaacaaactg gtcgggaatt tgggcaagct
     7381 ggcgctgatg cctgcgcagc catcttccag gcattgcgac atgtagccgg taaaatcggg
     7441 agtcatgccg agcgaggagg gcacgataac cgggtgcagg ccgaagttca cgagcatcgc
     7501 gagcggtgcc tgcgaggccg tgtacactgc gatcatgcca attcgaggat ccaagaattg
     7561 gtccgacgca gggcattgct gcttgattac cgagccggcg ctctgccacg cgttgtctcg
     7621 ccgattgatc aaaggacccg cgtcgttggt cgcttcgaag gtcgtggtgc cgacggccac
     7681 cttgacgggt acgagcgagg cgtatgttgc cgcgaccttc tccgcgatac tgtcttcgat
     7741 ccactgcacg tacgagtact cttgtgccgg gtactcgtac aaagccggcg cgggccctgt
     7801 aaacgagcgg tcgtcgtagg ttggcgccgt gtagaggttc agagtgtggg ggacggtgtg
     7861 ggagtgcgtc gcggcgatca ccacgttgcg cgtcgggatt cccgtgcgag cactgattag
     7921 atcacgtagc cggcccgggt tcacgcccac caactcgacg ctcatcaaga ccgccacgtt
     7981 gcgcgagccg cccccgagca agatgaaccg cgccatcggc ggcggggagt tcggaaagcg
     8041 cttgaacggg accggcagca agtctgcgct cggaaacaag ggggccgggc catccgcata
     8101 gtcgaggctg gtcttgtcgg cgccaccaac caagctcgag tagatgtagt cgatgcgctc
     8161 gttcttgtcg cgcgcgcagt cgatgaatcc gttgccgttg ccgtcgacgg tattgccgaa
     8221 gcgatccccg agaccgatcc cttcgacgct cttggtgagc ttcacgtccg catcgcccgc
     8281 ggcgagcagc gcgacatcga acgcacacgt ccgggggtcg acctgcgtgc gactctggga
     8341 gatgatcttc cggttcgtgc tgccgaactt ctcccagaca acgagcgcgt tcgcaggcgt
     8401 cgctgcgcaa tcaatcgccc gactaaactt tacttgaacc gggatgacgc ccccgctacg
     8461 gacttggtcg cgcgtaatat cggcaatcga gacgaccgac gtcggcaaca cggaggtcca
     8521 agtgatatcg ccatcgttgg cattgggtcg aattgcaatc agatccttca agcccccgtc
     8581 gttcgtggca tcggcgagcc ataccctgcg cttgaaggct ttgtcgaaat agccaccggg
     8641 aacctgaccg gattctccga ggtcactcac gccaacgcgc aacgaaggca gaaaagaacc
     8701 ggaggtcgtc ccactggtat cggactcttc taccagagat ttggcgaccc atacgccgcc
     8761 cgcacggtcg acaccaacca cgtccgcacg cttgtctccc gatacgtccc ccacccaaat
     8821 gcgtgggtga tatgcgttcg agagccagtc gttttgcgtc tttaatacgg agccgtggag
     8881 ccagaccttg ctcactaggc tccagggaga agactgagcg acccaaatgt cgccatcccc
     8941 ctgtccagga ggggcaatgc ccaccacatc gctccggcca tcacccgaga cgtctgcgag
     9001 ccagatgcgc ggctgcgagc cgacgtcgaa ccaaccggcg ttccggcgaa acaccgtcgg
     9061 gccgagcgtg atcgtggaat cgaaggaaac tgtcgttccg tttcccaggg agcgtgagag
     9121 ctgcacttgc ccgctcggcg cgatcccgac gatatcactc accccgtcgc ccgtatagtc
     9181 gctgacccat acgttatcat gataggcgag caagccgttc gagtcgcgcg tctggaagta
     9241 tccatcgctg ttcttgaagg cactcgggaa gggcccggcg acggcgccga agccgttcgt
     9301 ggtgcccggg tagacccaga tatcacccgc gttcgagaca cccaccaaat cgagtttgtg
     9361 gtcgccactg acgtctgcga gccaaacgcg agttcccgcg tatgcattgg cggagtcggc
     9421 cgcatcccaa aagccgtctc cgacgttcgg agtcgccgcc gcgaataccg tgctgcgcgc
     9481 agtcttctgc acgaatccag tgcccgtacc tagatagaag tagactgttc ccgcatcgtt
     9541 gacccccacg atgtccgcaa tgccgtcgtt cgtaacatcc gtcacgaaca cgcgttgacg
     9601 atagtcattc gtgaaccagc gagccgtggt ggtaaatcgt gtcgcgccga aaaggacggc
     9661 ggaattgtac tgaccgttcg tgcctgtcgc gacccaaacg gttccatcga agccaatccc
     9721 gatcacgtca ccaacgccgt cgccgttggc atccgtaacc cagatgcgat cgcggctgcc
     9781 cgtttcgaag aagtcgcccg cagtcgtgaa gaaagagccc gcaccccaag tctgggtcgt
     9841 tcgggatccg caactagtga tggcttgtga cgcgtggaac gccgggtcgc tcgccctgac
     9901 gggtgaactg agcgatgggc tgctgccccc ctcgcaaccc gaaagccacg gcccgatcaa
     9961 cacgcacgcg atcgtccaac cgtgcggcac acggtacctc agactccgta gattggtcaa
    10021 catgcgggcg ccgcacagca tgcgcagtgc catcgccaga ggtggaccct tcggcttctg
    10081 tttcccgcgt tacgcgcagt tctagcgggc tcccatgacc gtgggcggcc gcggattgac
    10141 ctgatggacc actttggtgg tgcatgtgta gcagtccagg tggagcacgg attcggccca
    10201 tcccgtaacc cgcttcacaa tgacagctgc ccccataaga acgaggcgag ttcctctttt
    10261 gcgcccgggt gaatgtccac ggatgaacca agcgataaga gcttgaaaga caacgcgacc
    10321 tgaagacgcg cgccgggcgc ggctttgccc gatcccagcc gcaataaggg tccagctcct
    10381 agggcaagcg atggcaggaa aacccccgag tcagttccgg cttcgaacat ccatgccgta
    10441 gacgtgcggc gctcgggctc gtggtctaca accagagagg tcgagaccga actaccaagt
    10501 ccaacttcat ccaccaattg gccgcgcatc gcagccggaa ctgccaattc gaagctgctc
    10561 cgctggccgg taccccgcgc aggaccatgc aaaccttgcg aagctgatcc caaagacgaa
    10621 caccccaatc tcgcaagctc gcctagtacg ctgccaacgg cacatcactg ccacctagcc
    10681 gctgacctgt caaatacccg aggcattggt gattaaccca agccatcaca accatggtga
    10741 gttcgcttgg aaactacctg tgaagtcgcg agtcacctgg gctcggctcc gtcgtgtccg
    10801 cccgttgagc tttaacccac gtcagttgca atagacgagg cagcgcaagc tctgggctcc
    10861 ccaactcgtt gggttccgcc atgctgacga gctcgaatcc tgcggcgcga gcggccatga
    10921 cgtagtcagc tgtcatatgc aagtaggctt ccactcgaac gctctcaccg tcgttctgga
    10981 agcgcgccgt ggtgcctcgt aattgacggt acgggtggag ctcacacaaa tacaacgagc
    11041 cctgcggact cagcactcgc gcggcctccg agaaaatagg agtgagattt tcgatgtgct
    11101 cgagcaccaa gttgcccacg acgacgttcg ctctgcccgt ggacaccggc caaggctcgc
    11161 gtatgtcatg ctcgatgaat cgaacgtgag gcgctgggca acgttgccga gctcgctcga
    11221 gcatgggggg agtgagatcc aaggcaatca cttcgctcgc ctgctcggcg aggaaggcgg
    11281 tattcttgcc ggtaccagcg ccgatctcga ccacggatgc gcctcgcaat ggcagccccg
    11341 cgctacgcaa gacctctgca tctagatccc gagtgacgtt ctcgtcatcg tcataacctg
    11401 cggcccacgc ttcgtaggcg atccttaccg gaacgccttg cggcggcttc gatgatgttg
    11461 gttcggtgga catatcaagc tcggctctgc ctggactcta tctcagcgcc gtctcgacac
    11521 aagtatggcg acggggcctc tcggtcacgt gctgagacga cttcgcaccc gaacggcacc
    11581 caggtgagac aatttttcag ggaccgtaac gggattcggt tcgatttacc actaggcagt
    11641 agaccaaaga gcgatcgaac caagccatga atcctgacgc cgtacccaat ctccaccgcg
    11701 ttgcatctcg agaagcgtcg ttagccggtg gccgtggccc gctcgcgctg ggggcgtggc
    11761 tggtcgcctt gctctcgagc tcggcgcccg cggcccaggc caagtcgttc atcgactacc
    11821 tcgagccgac gcccatcact tgctccccgc tcagctcggc ctcttggggc gtggcgggcg
    11881 tattgccgcg tgacctttgc aacggcatcg agagcgcgaa gggcgcggga gttccgccgg
    11941 actattacta ctgggacggg cagataataa ggaccaaaga cggcaagtat catatgttca
    12001 tgagcacctg ggcgggctca gctgggttca atcccgggtg gacgggctcc gacgcttatc
    12061 acgccatcag tgaacagggg gtacttggac cgtacaagag acaggggttc gtctacacga
    12121 acaacgggtc gcacaagggc cacaacgtca ccgcgctgga gctgccggac ggcagctatg
    12181 cggtggtcgt gagtgaaatc gtgcccttca cgatctacaa gtcgaactct ctcgacgggc
    12241 cctggacgag ttgcacggcc tcaatccaaa cgaatggcgt caacgccgga actgactcgc
    12301 actatgactc gaacgtcagc ttgacggctc gccacgacgg caaattcgag atcgtgcagc
    12361 gccacgggct gatcgccatc gccgacacgt tgtgcggccc ctacaagatg cagaaaccga
    12421 cgtgggccta tccggccgcc aatcggccat ctatcgacag catctatccc cgccggacat
    12481 cgatcccaga tccgtcgatc acgaatccca cctatacctg ggaagaggac ccccacatct
    12541 ggatcagcgg cggcgtttac cacgtcctgt attcgggatc tggggatcgc atcggctatc
    12601 acctctattc gccggacggg atcaacaact ggaaggacgg aggcttggcg ttcaatccta
    12661 ggatggcgga gaagatcttt tgctaccaag acagcaccac ttgcaacaag tggtacaaga
    12721 tggagcggcc gggggtcgtg ctcgagaacg gccgtgtgac ccacgtgacc tgggcggtgg
    12781 cggacgtgga taaggacaac caaatcccgg cgggctccaa tcacggtagt aaggtgattg
    12841 tcgtgccttt cgacggggtc gcgttcgatg cggactatgg cgtgaacggc aacgccggcg
    12901 cagggggcgc cgccgcaatg ggcggaagta cgagcgcagg aggaagagcc ggaggcggcg
    12961 gattgcccgg aagcggcggt acgaccagcg ccggagcagg cagtggcgga acgacgagca
    13021 gcgcgggcgc acagactgga ctcggaggcg ccgggccggg tggtaccggg ctgggcggta
    13081 ctgggctggg cggtactggg ctgggtggca cgacgggcgc ctccggttcg gcggggctgt
    13141 cgagcaccgg cgggagcacg gatcgcgtcg caagcggcgg caagacgaac gcggccgcag
    13201 gcggctcaac gggcacgagt gaagccaatg gcggtctcac cgcatcggga ggttccgtga
    13261 ccgcgggaaa tagcggcaat tcggcctcca gcgtagcgaa agactctggc tgcagctgct
    13321 cgataccctc gaactctcca tccggcgcgt ggggttcagg gctgctgctg ggtgggttag
    13381 gaacgttgct cgtcaagcga agacgtcagc gacgttgagt gtgcacttgc atctgggttg
    13441 catacggccg ttcgatccgt ccctacgcca aaagtccttc taaacaaatc aatcgaaccg
    13501 gtcacgaatc ttctgaacaa ggcggcggct ccaagcagat ccccaggagc cagattttct
    13561 ttcggattgc gtagcggacc cgcgtcccac gcgatcgccg aaactcgagg aaaaatccat
    13621 tgaagccgcc tcgtaactac cgacagtgtg cgacatccga cgggcgtgcg acacggattg
    13681 cgacgacagc atcgctccgc gccgtcctct tgcttgtgct cggctgtagc tccggcgccg
    13741 tcacggaagc cccagagtcc ggctccctgg gtggtggagg cccgcaaggc aactcaggtg
    13801 caggcggcgc agctgctaac gccggtcaag gcgcggtcgc tcaaggcgcg aacggccaag
    13861 gcgcgagtgg ccaaggagca atgccgattg ggggagccag cgcggcagga gccacaaaca
    13921 ccggtagtgc gggtcaaaag gatgatccct atgcgatccc atcttctccg ccgaacagcg
    13981 tcgtagtgcc gactccgcgc gtcgcacggc tcagtcggtt gcagtggtcg aacgcggtgc
    14041 gcgatctgct gaagttagcg gacatttccg aggtggacag tggggtcacc ggtgacgcgt
    14101 tgatcggctt cgataacgaa gccgagtctc tgttcgtgac cgaacagctg cgacagcaac
    14161 tcgccacggc cgccgagaag ctggccagta aggtgacttc ggacgcgagc gccctggcgc
    14221 ggctgcttcc cgcgaatgcg ccgagcagcg ggagcgcacg agcccgcgcc tttgtcacta
    14281 cgttcgggca acgagcattc cgacggccgc tcaccgacgc agaagtgacg acacatttgg
    14341 gtctgttcga tcaagggcca acgctctacc cgggtgtcga tgcgtttgcg gcgggcgcca
    14401 gcctggtgat ccaggcgatg ctgcaatccc ctcactttct ctaccgcacc gagctgggta
    14461 ccgccgcggc cggtgcggcc acagtggctc tcagtgactg ggaggtcgcg tccaaactcg
    14521 cgttcgcgat caccaacacc atgccggatg acgcgctgct gagcgccgct gcggcggggc
    14581 agctgcgcga cagggcgagc atctcggccc aagcgcagcg cttgctcgaa ggcgcgaccg
    14641 gtacggcggg gctccgtaac ttcaacctgc aggtttatcg gctcggcacg tacgacggca
    14701 tcgagcggga cgcgacgcgc ttccccgact tcaaggtcaa cacgcccgcg gccatgaaag
    14761 cagaggtgtt gcagttcatc gactggctct tcacgcaggg gcgcggaatc aaggatttct
    14821 acaccacccc tgttgggttc gtgaactcgc tactcgcgcc tttgtacggc gtctcgggca
    14881 acttctcgag cgaacccgcc gctttgagca aagtcgatct cgatccgacg aagcgctcgg
    14941 gtctgctcac ccaggctggt ttcttatcgt cctatatctc ggtcggggat gagccggaca
    15001 tcattcatcg tggtgtgttc atcgccactc gcctgttgtg caagacgctc ccaccaccgg
    15061 atccggcggc ggccggcaag ggcctcgtcg atacgcccaa cttgaccaat cgcgagcgcg
    15121 tcgaggcgac gacgggcaag ggcacgtgtg gcgccgtttg ccacggagcg ctcttcaacc
    15181 cgctcggcta tgccttcgag aactacgacg cggtgggcaa gtaccgaact caagaccaag
    15241 gcaagccggt agacgccgcc gacagctata tgctcgacgg gcagctcaag agtttcaaga
    15301 acggtgtgga gctctcggcc ctactcgccg aggccaaaga aacccacagc tgctatctgc
    15361 agaacatgct gagctacctg cagggtcagg agctctccga gcagcagcgg ccgttggtag
    15421 actactacgc acgcttgtcc cgcgctggaa aggtctctct tcgtgatctg gaacttcaga
    15481 tcgtgaccag cgaagctttc ctgaatcgcc gtccctgaag gtgcaaccaa tgaatagcga
    15541 agaggctcgt aagatgaatc ggcgactggt gctccgcggg gcctgcggac tcatgttggg
    15601 attgccgctg ctcgaaacct tcatgccgcg ccgagcggag gctcagacct ctacacgctc
    15661 accgttcgtg ctcatcgtgg tcggcgacaa cggggtggtt caggccggcg taaccctgag
    15721 tggaggcggt gaaccggaaa agttctggcc cacccagacc ggagcattga ccaaagacaa
    15781 gctactcgcc gacaaggcct ctcgctcgat tggcgagctc gcggactacg ccgagaagct
    15841 gctgatcgtc cgcggcatca acctgcccta caactcgacg ggctgctcgc attcggcagc
    15901 cgacgcgcag atcctcaccg cggccaagat cacggccggc agcaccaact gcaaggctct
    15961 cggcatctcg gtggataccg cgatcgccaa agccaagaac ccccccgggc gagacccgct
    16021 cgtgctccat gcgggcatgt tctcaccggg cggcactggc ttcgacattc ccgggtacgt
    16081 ctcgtacatc acgccgcagc agccacgcgt ctacatcgat tcaccgtaca aggcgtacca
    16141 acagatcatc ggcgccgtgg gcaacggcac gaccggcagc tcgacggacg atccagcgca
    16201 ggcgctgcgg gccgcgcgta gcaagagcat caacgatata ctgcgaccgc agatccgagc
    16261 gctgctggcg cggcaggatt tgagtcagag cgaccgtgac cgcctggacc agcatctgac
    16321 tgcgatccgc gatatcgagg tcaaggtttc gatgacgacg gcctttacga tcccggacgc
    16381 ggacgtcgcc acgatgaagt cgatggatcc caagccgtac gatcagtcca cgcgcgacgc
    16441 ggcggtcaag ttgttcatgg agctcatggc gttcgctgcc gcctcggact acacacgcgt
    16501 cgcggtgctc aaggtgggcg accgcatcga cgaccatgtg tacacgtaca acgggaaatc
    16561 cgcgaagttc cacgacgtct cgcatcgcca agtcgcgaac gcggtcgact ttcatcacta
    16621 catcgaccgc atgatgctca atcaatacaa gtatctgctg gacacactgt cgaagtacaa
    16681 cacgccctcg gggccgctga tcgatcaagg ggttgccatc tggacgaacc aatgcgctac
    16741 cggagcccac tcgttcagca acatcccctg gattcaagcc ggcactgcca acggcttttt
    16801 caagcagggt gagtatctcg tggtcggcaa cggacagcag cccggcggca gtcaagacgg
    16861 caaaggcggc gacggctctg tctccggcat caacaagatg ctgaataccg ttctgaccgc
    16921 cgccggcgtc accaagacgg gcggggcccc gaccgacgat ttcggagagc cgtcgctacc
    16981 caagggcatc ttcaacagca tgctcgcgtg ag
//
LOCUS       DASTKU010000278        10597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_501129, whole genome shotgun sequence.
ACCESSION   DASTKU010000278 DASTKU010000000
VERSION     DASTKU010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10597
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501129"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFQ61_10025"
     CDS             <1..253
                     /locus_tag="VFQ61_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954833.1"
                     /translation="ALSGRIARISSDLASPYELREAFGEGAQLPSPSYRVEMSVVDDD
                     NLRRLSPRIRPGMLVTARFTLRRRTLVALVLDPLRRWLR"
     gene            274..2421
                     /locus_tag="VFQ61_10030"
     CDS             274..2421
                     /locus_tag="VFQ61_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HET9954834.1"
                     /translation="MSWFRRVPYIAQMELTECGAACLAMVLGYWGQHISLVEAREACG
                     VSRDGATAYAIAKAAQEFGLDVQALSLDAESLPELGVPAILYWEFNHFVVLESANAKG
                     GVVVDPSLGRYFVPSDRLKASFTGIAICARPGARFQPRKARRPSLNRYRDLLRKSLPG
                     LGVVFGASLMLDCAAFVFPAATQILVDLVIKPQQSLWLWVLALSLFAATLVKGTLVAL
                     RSWVLQSLQFVLDLHLMTGFVDHLLHLPLAFFQQRTPGDLARRVQDNVALREFFSNRS
                     IAALLDGLLLLGYASLMLAYHSLLGAIVVGLGMLRVVLLLGMRGQIQQLSVTELAAYG
                     REAGALVEALSAPEATKAFGTERRMLNRYTERLVLRANAGTARRRLTLGIGFLMIFLD
                     GAGKAMIVLFGGREVLADRMTIGVFSAFLMLQALFAGPLESLVSVLNDLQHALKQLQR
                     LDDVMETPREPSGARHPGKLRGEIRLENVSFRYDPNGAFVVRDIDLCIRPGEKVAIVG
                     LSGAGKSTLARLMLGMHLPSSGRVLIDGQDLRELDLQEVRRQFGVVLQEAFFLNDTVR
                     ENLSLAGNDLPIQALRRAAHVACIDDALEALPEGYETRLGRSGSRLSGGQRQRLAVAR
                     AIAHEPAVLLLDEATSSLDLANEARLHANLARLSCTRIVIAHRLATVLDADRIFVLDG
                     GSIVAEGKYDELAARPGLFHDLVRTLTPELAAE"
     gene            2418..4511
                     /locus_tag="VFQ61_10035"
     CDS             2418..4511
                     /locus_tag="VFQ61_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET9954835.1"
                     /translation="MKKLTAEVHPSNQNSVQQAAERTSSAAHANAVLESRVECAGEFL
                     RLRGIRVTRQQLREALVEDSSLAALAEEVTRRGCPVRLLHCQPEDVGCLDLPTLVGLV
                     DGSSAVLVRMTLRSVYLRLDDGRTVRVYRKRLGEVWTGEALELAPQLPAALSFRARIW
                     AILQRHPRVLAQFLLASGFFQALALGVPLLLRTALDTALPDGAESMLYLLTLGIVMGA
                     ASQAWFAWFREAALRFLHARVLTSLNRGLLEHVLHLPFSYHQQKGLGELTQVVTAGDQ
                     VSRLATDVALTPLTDGVLGLMSLALLVYLLPSAALVVLVACLVFVAGAVVVGKRQGSY
                     QTQELAAQAEQNGFLLELVAGAQVIKATGAEQPSVVRWIQHLAKERWPSLRRQRLGLW
                     LEVGGECLRNGLLMFLLAWGGRQALAGALSLGTLIAAIQLAEGFAGSTFRVADACVKL
                     YAARAYAITLENALARPTQPSLKPALDSRGLGDDAIVLEDVWFRYEPGSPWILKGFSL
                     RVRAGEEHRLMGPSGCGKTTLLRLIAGLYEPERGAVLIHGVDASVARRRVAYLPQETY
                     LFQGSIFDNLRVLSGGASRERILQAAQLTGLDELIRTLPMGYETVLPPGGYTFSGGQR
                     QLIALSACVASERNVLLLDEAMSHLDRLTQARLVQSPLFEGKTLVSVVHEVVAPEPSS
                     TASRVTIDEFDALPA"
     gene            5417..5749
                     /locus_tag="VFQ61_10040"
                     /pseudo
     CDS             5417..5749
                     /locus_tag="VFQ61_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5647..7089
                     /locus_tag="VFQ61_10045"
     CDS             5647..7089
                     /locus_tag="VFQ61_10045"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET9954836.1"
                     /translation="MAPAAAASWHAGPGRRKAWAQGEARCQGPSHPRARAQDSTPREG
                     TSHLSEARRAPKKSARVAWDCAATASGRRGALMALAEHVEPPVPITRACKALGISRAT
                     LYRSTTPPVPRLPREPRPVPRRLDEAERHAIVEALHSPEFVDQPPHEVYAALLSRGVY
                     LASIRTMYRVLAALGETQERRAQRTGVRHTKPTLEATAPNQVWTWDITKLAGPAPGIY
                     YCLYVILDMFSRYVVGWLLAESESTNLAKQLFAETLHRHGVEPNHLTVHSDRGSTMKS
                     EGLAQLLGLLGVTRSFSRPRVSDDNPFSEAQFKTLKYQPDFPERFDSLQHARAYCQPF
                     FGWHNDDHHHSGLALFTPADVFHGRVPAVAANRQAALDAAYVAHPERFPNGPPRVPLP
                     PEAVRINPLHASSDTSSPESSSPSAPHERHATGNVDGRHGARHGVMSDNSHITPTAKP
                     KPSSTLKHSAAHHPAAEPFSECRAQTPFPS"
     gene            complement(7524..8312)
                     /locus_tag="VFQ61_10050"
     CDS             complement(7524..8312)
                     /locus_tag="VFQ61_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9954837.1"
                     /translation="MTRKTAEQRDPNDPRERLRRLGLYGLIARWPEIATQPWLEQLLG
                     IEEAERKQRSLDRRLRNAKIGTFKPIDRFDWAWPKKIDRQAIEELFSLAFLDDGANAI
                     LLGPNGVGKSMILRNLAQHALLRGHTVRFTTASDMLAELAAQDSATALARRLRRWSLP
                     RLLCIDEVGYLSYSSRYADLLFEVVTRRYDAGRSIVVTTNKAFNEWSEVFPHAACTVT
                     LVDRLIHRSEIIEIDGDSYRLKEAKERTAAKVKQRKTKHGHTHP"
     gene            complement(8293..9795)
                     /gene="istA"
                     /locus_tag="VFQ61_10055"
     CDS             complement(8293..9795)
                     /gene="istA"
                     /locus_tag="VFQ61_10055"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HET9954838.1"
                     /translation="MIDKATEANILRLYYAEKWRIGTIATQLHVHHTTVQRVLAQAGL
                     EPQRVSQRPSMSDPYTPFIVETLEKYPSLCASRLFQMVRERGYPGGPDHFRRVVARFR
                     PRPKHEAFLRLRTLIGEQAQADWGAFGKLAVGRALRSLWAFVMVLSWSRQIFLRFYLS
                     AAMPSFVRGHADAFAFFRGVPRVILYDNLKSAVLERAAHAIHFNPRILELARHYKFEP
                     RPVAQARGNEKGRVERAIRYVRTSFFPARKYTDLDDLNRQAFDWMTGVAADRRAHEDP
                     SQRVRDAFSLEQSKLLPLPDEPFPSDEIVGVESGKTPYVRFDLNDYSVPHTHVRRSLT
                     VAASLDTVRVLDGTHVLATHSRCWDRAQQIEDPSHIQQLVSQKRRARAHRALDRLSSA
                     VPSAQALLGQAAERGGNLGNITGRLLVLLEQVGPSELEHAVACAVERNLPTVGAVRQL
                     LDQRFADRGLPPPISARLSNNERANRVTVRNHLLATYDNLRKTDNDQEDS"
     gene            complement(9792..10151)
                     /locus_tag="VFQ61_10060"
     CDS             complement(9792..10151)
                     /locus_tag="VFQ61_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954839.1"
                     /translation="MFQPWQCTLHVTSTAATLLLTDPTHDLLKARLNPCPSHPRALLT
                     LLEGLSLWGGDRLRVALSVEEGCLRGLGSTLLGDELWPAESALVQYDVVRPARPKRLD
                     GIGDFRQLRRVARRCGP"
BASE COUNT         1904 a         3319 c         3364 g         2010 t
ORIGIN      
        1 cgcgttgagc ggccgtatcg cccgcatctc gagcgacttg gcttcaccat acgagttgcg
       61 ggaagcgttc ggcgaggggg cacagctgcc gagtcccagc tatcgagtcg aaatgagcgt
      121 ggtcgatgat gataacttga gacgcctcag cccacgcatt cgtccgggca tgttggtcac
      181 ggcgaggttc acgctgcggc gccgcacgct cgtggcgctc gtgctggatc cgctacgtcg
      241 ctggttgcgc tgacacgggt tgcgggcaac agcatgtctt ggtttcgccg cgtaccgtac
      301 atcgctcaga tggagctcac cgagtgtggg gcggcatgcc ttgccatggt gctggggtat
      361 tgggggcaac acatctcgct ggtcgaggcg cgcgaggcct gtggtgtctc gcgtgacggc
      421 gccaccgcct acgccatcgc gaaagcagcc caagagttcg gactggacgt tcaggccctg
      481 agcctcgacg ccgagagttt gcctgagctc ggggtgccgg cgatcctgta ttgggagttc
      541 aaccacttcg tcgtgctgga gagcgcgaac gcgaagggcg gagtagtcgt cgatcccagt
      601 ctcggccgct acttcgtgcc gagtgatcga ttgaaggctt cgtttaccgg aattgcgatc
      661 tgtgcgcgac cgggagcacg cttccagccg cgcaaagctc gccggccaag cctgaatcgg
      721 tatcgcgatc tcctgcgtaa atccctgccc gggctgggtg tggtgttcgg cgcttcattg
      781 atgctggact gcgcggcgtt cgtgttcccg gccgcgacgc agatcttggt cgatctggtg
      841 atcaagccgc aacagagctt gtggctgtgg gtgctcgcgc tgagcctgtt tgcggcgacg
      901 ctagtgaagg gcacgctggt cgccctacgg agctgggtgc tgcagagcct ccaattcgtc
      961 ctcgatctgc atttgatgac gggcttcgtc gatcatctct tgcacttgcc tctcgcgttc
     1021 tttcagcagc gcacgccggg tgacttggcg cgccgggttc aggacaacgt ggcgctgcgc
     1081 gagttcttct cgaaccgctc gatcgcggcg ctcctggacg gtctgctctt gctcggctac
     1141 gcgagtctca tgctcgccta ccacagtctg ttgggcgcga tcgtggtggg actcggcatg
     1201 ctgcgggttg tgttgttgct cggcatgcgc ggacagattc agcaattgtc ggtcaccgag
     1261 ctcgcggctt acgggcggga agcgggcgcg ctcgtcgagg cgctctccgc cccagaagct
     1321 accaaagcgt tcggcaccga gcgacgcatg ttgaatcgat acacggagcg cttggtgctg
     1381 cgcgccaacg ccggtaccgc gcgccggcgt ttgaccttgg ggattggctt tttgatgatc
     1441 ttcttggacg gcgctggcaa agccatgatc gtgttgttcg gcggacgtga ggtgcttgcc
     1501 gatcgcatga ccatcggcgt gttcagcgcg ttcttgatgc tgcaggccct gttcgcgggc
     1561 ccgctcgagt ccttggtgag tgttttgaat gacctgcaac acgcgctgaa acagctgcaa
     1621 cggctagatg acgtgatgga gacgccgcgc gagccgagtg gggcgcggca cccgggcaag
     1681 ttgcgcggag agattcggct cgaaaacgtt tccttccgct atgaccccaa tggtgctttc
     1741 gtggtgcgtg atatcgactt gtgtatccgc cccggcgaaa aggttgccat cgtgggcctt
     1801 tccggagctg gcaagtcgac gctagcgcgt ttgatgttgg gcatgcactt gccgagctcg
     1861 ggtcgcgtgc tgatcgacgg ccaagacctg cgggagctgg acttgcagga agtgcgacgg
     1921 cagttcgggg tggtattgca ggaggcgttc ttcttgaacg acacggtacg agaaaacctc
     1981 tcgcttgccg gcaacgactt acccattcaa gccctccgtc gagccgccca tgtagcctgc
     2041 atcgatgatg ccttggaggc tcttccggag ggttacgaga cgcggctcgg tcgctcgggg
     2101 agccggcttt cgggagggca gcggcaacgg ctcgcggtgg cacgggccat cgcgcatgaa
     2161 cccgcggtgt tgctcctcga cgaggcaact agctcactcg atttggcgaa cgaggcgcgt
     2221 ctgcacgcga acctggcgcg tctctcgtgc acgcgtatcg tgattgcgca ccgcctggcg
     2281 acggtgctcg acgccgatcg aatcttcgtg cttgacgggg gcagcatcgt cgccgagggc
     2341 aagtacgacg agctcgccgc gcgcccgggg ctgttccacg acttggtccg cacgcttacc
     2401 ccggagcttg cggcggaatg aagaagctga cggcagaagt gcatccgtcc aaccagaact
     2461 ccgtccagca agcagctgaa aggacctcta gcgccgctca cgccaacgcc gtgctcgaga
     2521 gtcgcgtcga gtgcgccggg gagttcctgc ggctgcgcgg aatccgtgta actcgacagc
     2581 aactccgcga agcgctggtc gaagatagtt cgctggccgc tttggccgag gaagtcacgc
     2641 ggcgcggctg ccccgtgcgg ctgctgcact gtcaaccgga ggacgtgggc tgtctcgatc
     2701 tacccacgct cgttggtctg gtggatggct cgagcgcggt cctcgttcgg atgaccttgc
     2761 ggagcgtgta cctgcgcctg gacgatggcc gaaccgtgcg cgtctaccga aagcgactcg
     2821 gggaggtgtg gacgggcgag gcgctcgagc tcgctccgca gctgcccgca gctctgtcat
     2881 ttcgagcccg catctgggcc atcctgcagc gtcacccacg cgtgctcgcg cagtttctct
     2941 tggcgagcgg attcttccaa gctctggccc tcggtgtgcc tctcttgctg cggaccgcgc
     3001 tcgacactgc gttgcccgat ggcgcagaga gcatgctcta tctgctcacg ctcggcatcg
     3061 tgatgggcgc cgcctcacaa gcctggtttg cgtggttcag ggaagcggcc ttacgcttct
     3121 tgcacgctcg cgtgctcacc tccctgaacc gggggctgct cgagcacgtg ctgcacttgc
     3181 cattctctta ccatcaacaa aagggactcg gtgaactaac tcaagtcgtc accgccggtg
     3241 accaggtcag ccgcttggcc accgatgtgg ccctgacccc actcaccgac ggtgtgctcg
     3301 gtttgatgag cttggcgcta ctcgtatatc tcctgccgag cgccgcgctc gtggtgctcg
     3361 tggcttgttt ggtgttcgtg gctggagccg tggtcgtggg caagcgacag ggcagctatc
     3421 aaacccagga gctcgccgca caagcggaac agaacggctt tctgctcgag ctcgtagcgg
     3481 gtgctcaagt catcaaagcc acgggcgcgg agcagcccag tgtggtgcgt tggatacaac
     3541 acctcgccaa agaacgttgg ccgagcttgc gacgtcagag gctcgggttg tggctcgaag
     3601 tcggcggtga gtgtctacgc aatggtctgc tcatgttcct gctcgcgtgg ggagggcggc
     3661 aagcactcgc gggcgcgctt tctctcggca cactgatcgc cgcaattcag ttggccgagg
     3721 gcttcgcggg gagcaccttc cgcgtcgcgg acgcatgtgt gaagctctat gcggctcggg
     3781 cttacgcaat caccttggag aacgccctcg cgcgccccac gcaaccgagc ttgaaacccg
     3841 cgctcgactc gcgtggactg ggtgacgacg cgatcgtgct cgaagacgtc tggtttcgct
     3901 atgagccggg ttcgccctgg atcctcaagg gcttcagctt acgtgtccgt gcgggggaag
     3961 aacatcgtct gatgggtccg tcgggctgcg gcaaaaccac actgctgcgg ttgattgcgg
     4021 gcctctacga gccggaacgc ggggccgtgc tcattcatgg cgtggacgcc agcgtggcac
     4081 ggcgccgagt ggcgtatctg ccgcaggaaa cctatttgtt tcaagggtcc atcttcgaca
     4141 acttgcgagt gctctccggc ggagccagcc gcgaacgcat cctccaagcc gcccagctca
     4201 cgggcttgga cgagctaatt cgcaccttac caatgggtta cgagaccgta ttgcctccgg
     4261 ggggttacac cttctcgggc ggccaacgcc agctcattgc gctcagcgcg tgcgtcgcct
     4321 ccgagcgcaa cgtgctccta ttggacgaag cgatgtccca cctcgaccgc ctgacgcaag
     4381 cgcgcctggt acagagtccg ttgttcgagg gtaaaacgct ggtctctgtt gtccatgaag
     4441 ttgtggctcc cgagccctcg tcgactgcat cgcgcgtcac gatcgacgag ttcgacgccc
     4501 ttccggccta aaagcgtcgc gatcgagcgt ctccgcggac agcgcatgca tcatccccac
     4561 gcccacgggg gccgccatgt cgaggtcccg tcgcgattgc gcaccgccag acccaaatgt
     4621 gaaaccacgc tcctgaccga ttgcgctttg gcggccaccc tgtcgacgtg cgcgactgcc
     4681 gtcgcggcga cccttggcgc agccttcgtg accaactcca aagcttcgat accgcgccgg
     4741 cccccgaaat acaaaggaac gactcgcctg ggcgttctgc ttatgagagc cgtccgaacc
     4801 tggcgctggc gcccgatcct cgtcgaagcg catcctacgc ggcgcaaacc cagaccagcc
     4861 taatcccaac cgctttatga cggcacaagc cacatcgcgg gggagccctc cgcccagaat
     4921 agcgccacaa ggcggcgtcg cccaagacgc atttagcagc gggagaagac agcaaggcct
     4981 gcagccttct ccccatcgac gcccggcgga aagcggtgca gggtgcaggt gaaaaggagt
     5041 taggcgagcc tgtcgcttac gcgccctggc cacagctgcc tacacagttg cgtgcgtcac
     5101 ttgcacggag tcgttgtaca aggctatcgt cgacgccacg ggaccgacaa tggcaagcaa
     5161 aaaggctact gaaaagcgca gtgcctccaa taaaccgagt cgaaagaatc aaagggagga
     5221 cgccgacgcg caaaagaaga aagcgagggt caaattccct ttgcacccgc tcgaagcgtc
     5281 cttgcgaatt ccgcgcgtta tcctcgaaca gaacgccgga aaaaaatgca cggattcaca
     5341 agcggcgaag ttcgtagggc ccctctgtgc caacgtgggt tgagacagtc tcctctggaa
     5401 atctctgtcg agaagggtgg agaatggtcg tgaccccttg ccgaagccaa ccaacataca
     5461 tccgagcgcc gcgaaggcgc aggccgagag cggcgccgcc cacgagagta cggctaagcg
     5521 cactcggcgc aagttcaccg cagccgagaa gctgcgcatc ctcaaagaag ccgaagcctg
     5581 caccgggcgc ggtgaagtgg aggcattgct gcgccgagaa ggcgtctaca gttcgcattt
     5641 gagcaactgg cgccagcagc tgcagcttcg tggcacgcag ggcctggccg ccgcaaagcc
     5701 tgggcgcaag gcgaagctcg atgccaagga ccgtcgcatc caagagctcg agcgcaagac
     5761 agcacacctc gagagggaac ttctcatctc tcagaagctc gtagagctcc aaaaaaaagc
     5821 gcacgagttg cttgggattg cgctgccaca gcctccggac gaagaggagc gctgatggcg
     5881 ctcgccgagc acgtggagcc gcccgtgccg attacgcgcg cctgtaaggc gctcggcata
     5941 agtcgggcaa ccctgtaccg gtctacgact ccgccggtgc cgagactgcc gcgagagccg
     6001 cgcccagtcc cgagaaggct ggatgaggcc gagcgtcacg ccattgtcga ggcgctgcac
     6061 agcccggaat tcgtggacca accgccccac gaggtttatg ccgcactgct cagtcggggc
     6121 gtctatctgg cgtcgattcg gaccatgtac cgcgtcctcg ccgctctcgg agagacccag
     6181 gagcggcgag ctcaacgcac tggggttcgg cacaccaagc ccacactcga ggccacggct
     6241 ccgaaccagg tttggacgtg ggacatcaca aagctggcag gcccagcgcc gggcatctac
     6301 tactgcctgt acgtcatcct cgacatgttc agccgatacg tcgtcggctg gttgctcgct
     6361 gaatccgaga gcaccaacct cgccaagcag ctctttgctg aaacgctcca ccgccatggc
     6421 gttgagccga atcacctcac cgtgcactcc gaccgcggct ccactatgaa aagcgagggt
     6481 ctcgcccagc tcctcggact gctcggagtc actcgcagct tcagccgccc ccgcgtctcc
     6541 gacgacaacc ccttcagcga agcccagttc aaaaccctaa agtaccaacc cgacttcccc
     6601 gaacgcttcg actcgctcca gcacgctcgg gcctactgcc aaccgttctt cggctggcac
     6661 aatgacgacc accaccacag cggcctcgcc ctcttcaccc ccgccgacgt cttccacggt
     6721 cgcgtccctg ccgtggccgc caatcgccag gccgccctcg acgccgccta cgtcgcgcac
     6781 cccgagcgct tccccaacgg gccgccgcgc gtcccgctgc cgccggaggc cgtccgaatc
     6841 aatcctctcc acgcgtccag cgacacatcg agccctgaaa gctcgtctcc ctccgcgccg
     6901 cacgagcgcc atgctaccgg caacgttgat gggcggcacg gtgctcgcca cggggtgatg
     6961 agcgacaact cacacataac ccccaccgct aaaccaaagc cctcatccac actcaagcac
     7021 tccgcagctc atcaccccgc ggccgagcca tttagcgagt gcagagctca gacaccgttt
     7081 ccctcataga attcatacgc cgactgtctc aactgcgttg acacgttccg ctttacaata
     7141 tttgagtttc gtgagcggcg gagaagccca ttgcgcggcg acggaattgg cctgccctaa
     7201 cgtgaattgg cggcgaccag ctacgccgca cgacgccgga gaagcttctt cttgcctcgg
     7261 cctttggcgg ggcctggtcg gtccgttctc agcagagggc gtgcagcgag cttctccaca
     7321 acaaacgcgt gtacgtcatc ccaatcggtt gctcgaggtg cttccatgtg cttgttgtcg
     7381 aattgccgaa gaccaggacg tcacagccgg cacttctaag cgcctccacg ttcttgggag
     7441 tgtcaccgag cgcgtgaaac cgcggaagtc cggtgaagag gcacgcgggc gctctgcacc
     7501 ggattcgcga cggaggatgg cgctcagggg tgcgtatgac cgtgcttggt tttccgctgc
     7561 ttgactttgg cggcggtacg ttctttggcc tccttcaagc gataactgtc gccgtcgatc
     7621 tcgatgattt ccgatcggtg gatgaggcgg tcgacgagcg tgacggtgca ggcggcgtgg
     7681 gggaagacct cgctccactc gttgaaggcc ttgttcgtgg tcacgacgat ggatctgccg
     7741 gcgtcgtatc ggcgagtgac gacctcgaac aacaggtctg catagcggga gctgtaggag
     7801 agataaccga cttcgtcgat gcagagcaaa cgaggtagcg accatcgacg caagcgtcgg
     7861 gcgagcgccg tggcgctatc ctgcgccgct agctcagcca gcatgtcact ggctgtggtg
     7921 aagcggacgg tatggccgcg gaggagggcg tgttgggcga ggtttctcag gatcatgctc
     7981 ttgccgacgc cgttgggtcc gaggaggatg gcgttggcac cgtcgtcgag gaatgcgagg
     8041 ctgaagagct cttcgatggc ctgtcggtcg attttcttcg gccatgccca atcgaagcga
     8101 tcgatgggtt tgaatgtgcc aatcttggcg tttcgcagcc tgcggtcgag gctgcgctgc
     8161 tttcgttctg cttcttcgat gcccagcagc tgctcgagcc agggttgagt ggcgatttcg
     8221 ggccaccgcg cgatgaggcc gtacagaccg agacgacgga gtcgttcgcg cgggtcgttg
     8281 ggatcgcgct gttcagctgt cttcctggtc attgtcggtc ttccgtaggt tgtcgtaggt
     8341 tgcgagcagg tgattgcgaa cggtgacgcg gttggcccgc tcgttgttgg agaggcgagc
     8401 cgagatgggc ggcgggagcc cgcgatctgc gaagcgctgg tcaaggagct gacgtaccgc
     8461 gccgacggtt gggaggttgc gttcgacggc acaggcgacg gcgtgttcga gttcactcgg
     8521 gccgacctgt tcgaggagaa ccagtaaacg accggtgatg ttgccgagat tgccccctcg
     8581 ttccgcggcc tgacccagga gcgcttgcgc gctcggaacc gcgctgctga gtcgatcgag
     8641 ggctcgatgt gcgcgcgcgc ggcgcttctg tgagacgagc tgctgaatgt gggaggggtc
     8701 ttcgatctgc tgagcacggt cccaacatcg agagtgagtc gcgaggacgt gtgttccatc
     8761 gaggactcgt acggtatcga gggaggccgc gacagtgagg gagcgacgga catgggtgtg
     8821 cggcaccgaa taatcgttga ggtcgaaacg aacatagggg gtcttcccgg attcgacccc
     8881 tacgatctca tccgaaggga acggctcatc cggcaagggg agcagcttcg actgctccag
     8941 cgagaacgca tcacgcaccc tctgcgaggg gtcttcgtga gcgcgtcgat cggctgcaac
     9001 ccctgtcatc cagtcgaagg cttgccgatt gagatcgtcc agatccgtgt actttcgtgc
     9061 tggaaagaag ctggtgcgga cgtagcgaat cgcgcgctcg acccgtcctt tctcgttacc
     9121 ccgggcttgt gcgacgggac ggggctcgaa cttgtagtga cgcgcgagct cgaggatccg
     9181 ggggttgaag tggatggcat gagccgcccg ctcgagcacc gcgcttttca gattatcata
     9241 caagatgaca cgcggtacgc ctcgaaagaa ggcgaaggcg tcagcatgac cacggacgaa
     9301 gctaggcatg gccgcgctca aatagaaccg cagaaagatc tgccgcgacc aactcaggac
     9361 catcacgaaa gcccagagtg agcgcagcgc gcgcccgacg gcgagctttc cgaatgctcc
     9421 ccagtcggcc tgcgcctgct caccgatcag agtgcgcagt cgcaggaagg cttcgtgctt
     9481 gggtcgcggg cggaagcgag ccaccacgcg ccgaaagtgg tcggggcctc cgggatagcc
     9541 gcgctcgcga accatctgga acagccgact cgcgcacaga ctcgggtact tctctagcgt
     9601 ctcgacgatg aacggggtgt acgggtccga catcgatggt cgttggctca cccgctgcgg
     9661 ctcgagcccg gcttgggcca acacgcgctg aaccgtggtg tggtgcacgt gcagctgggt
     9721 tgcgatcgta ccgattcgcc acttctcagc gtagtagagc cgcagaatgt tggcttccgt
     9781 tgctttgtcg atcatggccc acaccgccgc gccacgcggc gcagttgcct aaaatcaccg
     9841 atcccgtcga gccgcttggg gcgggcgggg cgaacgacat cgtactggac cagcgcgctc
     9901 tcggcgggcc acagctcgtc gccgagcagg gtcgagccga gcccacgtag gcagccctct
     9961 tccacagaga gagcaacgcg gagacggtcc ccgccccata gactcaagcc ttccagaagc
    10021 gtcagcaacg cccgaggatg gctcggacat gggttgagac gtgccttcaa tagatcgtgt
    10081 gtcggatctg tcagcaacag tgttgccgcg gtcgaggtca cgtgaagcgt gcattgccag
    10141 ggctggaaca tcgccaggag cctccattgc tccgtgctga gtcgcgtctc tcgcgaccgc
    10201 agcctcctca tcgatgctgg ttgccagtcc ctgggtcgag ccctgttgag tcactatctg
    10261 cgccggatac ttggcccgtg ccttcctatc tcgcagtcgc tgctgacggt cagcgtgcaa
    10321 tcgccttccc cggcgggtct gctggaatgc actcgagtga aggcgccact gtcttcggcg
    10381 ggccgcgtct gcacacatcg ccgagcagta ccgttgacct cgctcgcacc gtgggcagac
    10441 catgcagact cgatggcagc cagcacagcg tataaaccgc cagacctctt cggaaatgcc
    10501 agcgcacact gccctcccga aaatcagatc ctatttctgg acgaaaccga acgaacctgc
    10561 gatacgcccc tatggcgcgt cgcacactgc gcgatcg
//
LOCUS       DASTKU010000279        12827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1169865, whole genome shotgun sequence.
ACCESSION   DASTKU010000279 DASTKU010000000
VERSION     DASTKU010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12827
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169865"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4616)
                     /locus_tag="VFQ61_10065"
     CDS             complement(<1..4616)
                     /locus_tag="VFQ61_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HET9954840.1"
                     /translation="MKSSDERLLRLLKEARYALEAERKRRTEPIAIVGIGCRFPGGAA
                     GPRAFWKLLEQGVDATCPVPLERWDARALYDPDPNAPGKCYTQRGGFLDQVSGFEPEF
                     FGISPREAAGLDPQQRLLLEVTWEALEDAGIPADRLAGSATGVWVGLSLDDYARRCIP
                     TGHLERIDAYTALGNARSVAAGRIAYVLGLHGPVLQLDTACSSSLVAVHLACQSLRSG
                     ECELAIVGGVNVMSSPEASVALCRLQALAPDGRCKTFDAAADGYGRGEGCGVVVLKRL
                     AAARAAGDHIHAVIRGSAVNHDGRSNGLTAPNGAAQEAVLRSALTDAGVDPAHVGYVE
                     AHGTGTLLGDPIEVLALSRVYGAARAPEQPLVLGSVKTNFGHLEAAAGVAALIKTALC
                     LEHGQLAPHLHFHKPNPKIPWAQLPVRVATQNEAFPRGQGGAFAGVSSFGISGTNAHV
                     VLEAVSSIASGPHAVARSAELVVLSARTEPALKALARELSSQLAARPELSLAELAFSS
                     MTARAAMDRRWAFAVSSRAAFEAALEAALAAENAPVTTTDSISRPKVAFIFPGQGSQW
                     PGMGRSLLLEEPVFREAMQACDAAISRETGWSVLTELESGAQFDRIEVVQPLLFAMQV
                     SLAALWRSWGVEPDVVVGHSMGEAAAACVAGSLSLEDGARVICRRSKLLRRISGKGEM
                     ALVELSIDEAKAALRGFEDRLGVAVSNARHSTVLSGEPAALASVLATLEARGVFCRRV
                     KVDVASHSPQVDPLLAELVSQLTDLAPVTETIPIYSTVTGRALSGSEQTARYWADNLR
                     KPVRFADAVEGLIEQGFRLFVELSPHPLLLSAIEELRGQAGVAGTAVGSLRREQPERL
                     ALLDALGALYTHGMPLQPERLFPNGMIGGKNGLRRIELPTYPWQRENYWLEAPVHLAG
                     SAPESGHPLLGVRVATAAAFAVYETLISEREPGWLVDHRVGGEAVLPAAAILELVRAS
                     AEDAHDGLSCEVSELVLASPLVLRAGQTTRLQLVLSRDSDSDALSATLCSQPADAVTG
                     TAWTRHATARAIPVSADVSGAALPKVPVGTLRARCSTSVDVERLHAGFLTGGIQYGSA
                     FRGLRYVSRGEREVIAELELHSSLSAQGYGVHPALLDAALQASAALLPLEATGQAFLP
                     FGCERYRVQRAAADNALVHVELIDASPEHLVVDVTLCEPTGEVIAEIDALHARRADVP
                     RLIERASSSKVTPNRHELYRLSWREAELRGIDTPEKSTAPLSQQRQASRWGIVALGPH
                     SSGDLHESANLHERSNLQRLGEALSDLGAAVDHLDLAALEASAVEHVVCVWDVGEDAT
                     AATRAAIQALQIVQALTRRNRPTRLWWITRRVFSELTDGVSEPAVVSPAAATVWGVGR
                     TLRQEHPELNCKLIDCDANEPEFAASVARILNAELAAQDEEDELRYCRGRRYVARLST
                     VPGHRPSTDSRASVLSASGTVLITGGLGALGLMLARGCAERGARRLLLVGRRGAETPG
                     TTEALAELQRIGAEVEVAAIDIADAASLARLIAAIPAEFPLRGV"
     gene            complement(4613..8365)
                     /locus_tag="VFQ61_10070"
     CDS             complement(4613..8365)
                     /locus_tag="VFQ61_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9954841.1"
                     /translation="MATSAPGSVPATFVQVLQARAEGQPDRVAYTFLHEGEVEAKSLT
                     FGELHQAALAIAHRLSWLGARGERALLLYPQGLEFISAFFGCLYAGVIAVPVSLPSRK
                     RGFEGLRRVAADAGAKWLLSTGDLLQQITRELAETPSAYAPTFLDTEACAVTGVSQPT
                     TLPRVGPEELALLQYTSGSTGAPRGVAVTHGNLVQNQIQIDEIFRHDPDRCAVSWLPM
                     FHDMGLGTVLQTLRLGARCVLMSPQSFLQSPVRWLRAISQYRAASSGGPDFAYDLCVR
                     RIRPEERAGLDLSCWRVAYNGSEPVRAATLQRFAEAFASTGFRFEAFHPVYGLAEGTL
                     IVTGEPASQPPVLEHFDARELELGRAVKSSKESSSLVGCGHAWAGTELCIVDPESLHS
                     CAPGRVGEIWVRGVSVAQGYWGKPRESRETFGATRADGEGPFLRTGDLGFVLDDRLFV
                     TGRRKDLIIIRGRNHYPHDLESSVSTSHPDLVPHACAAFSIETNQGEQLVVVQEVART
                     ALRTLDAAAVVRAIRRAVSEQHALHTHAIVLLKPSTLPRTTSGKVRRKACREAFLSNA
                     LPSVVAWTSPLPQDAAAHADGERQARAVKADALIDWLRRHAADLINAYSNAPRSSTPS
                     ALLRDLAKQGLLGMQVEPEYGGLGLGNVDTMRVLEQLAAIDFALALFVGLNNYLGIQP
                     IAKYARVQVKSLLLPGLAHGQEIAGFAFEEPTGRTPPGGVGMQAQAEGEDAWRLFGTK
                     YLDGFAQGANIVNVFAHHDEPPGISAFVVSSGLDGLRHVNDGLSMGLLGFARDTVVLD
                     GVLVGRESLLGNFGSGLDIAREAMMQTRLAIAAACIGGMKRCAQLAGRETPLHGGING
                     RLTPNPITLSRLGSVTASITALECLVRRVAQATDQGFAVPPEAFAACKVLGPDLLLRC
                     IDDVMRVGAGGEPYRLSALHRDAGLLRNFDGPPEALSELTGAVVMAGDTTLRQLVEEV
                     FGAPTLTRSIDTALDAVRQRMKNLSGALARRAQRWGHTRAGELTSWIVLLAAVDGARK
                     LEPSPDLERAYAWADAQFQQALSAVRLGTPSETAMLDYSDVAATFAAYASTIGELHPD
                     PRAEPDVYRIGAWKRPPESEPLPSVAPTSEATAAREPALRELKGWVVSWLARHLQIPV
                     ADVETGRSFADHGLDSIAAVELAKALSDRVGRKLDETVLWNFSTIDALVEHLVGPPRV
                     SRSEPPPPPPPVTPNFEAERPSASLPVEVRLDDEMARLERELRSRS"
     gene            complement(8875..9984)
                     /locus_tag="VFQ61_10075"
     CDS             complement(8875..9984)
                     /locus_tag="VFQ61_10075"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9954842.1"
                     /translation="MKLRSALSLRFTAWTLLAATLAPSLAFAEGDPSVPISESQDRTL
                     EDLIQQGIRLRKAGNDEAALQVFHDAERRNPDSVRVLLNIAGAAAAVGKWIECDAYFR
                     KVSSHRDDPYYQRHRAAITAIENTLARKVGRFTASGTPLGAEIRLNGELIGNLNMAEP
                     QTVEAGTYVLEVSQPGYYRVRRQITIQAGILARESIDLNELPPAAASAPASAQAGGDV
                     SSPLVQMESPSSAPIQPERAPARQVEPTSSGRWFTWTLAGLGLAAAATGGVAFAIREQ
                     HAKRWNDDNRCLPTTGKTREETCGDERKSAETAERVAIVGGAAAVVLGTGALIHWLST
                     RSSTSSSASGSASDSLARSTTCGVGLLSIGCQGSF"
     gene            10548..10952
                     /gene="apaG"
                     /locus_tag="VFQ61_10080"
     CDS             10548..10952
                     /gene="apaG"
                     /locus_tag="VFQ61_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HET9954843.1"
                     /translation="MDRESRGHSAAVTDGVRVTVEARYSAEQSLPAARRYVFAYTVRI
                     RNEGREGVQLRSRHWILTDSAGHTEEVRGEGVIGEQPVLPPGGQFEYMSAAVLKTPRG
                     EMRGSYQMVTWNGRSFDATIAPFQLALPYSLN"
     gene            11080..12654
                     /locus_tag="VFQ61_10085"
     CDS             11080..12654
                     /locus_tag="VFQ61_10085"
                     /inference="COORDINATES: protein motif:HMM:NF000658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HET9954844.1"
                     /translation="MSSSLELPAFKNRFVTELPGEADASRHSRAVHGACYSLVRPVPV
                     SAPSLVAYSRDLAERLRLDGGTLESSLWLGILSGNTVSADMQPYACCYGGHQFGTWAG
                     QLGDGRAITLGELVDREGCHQELQLKGSGRTPYSRTGDGRAVMRSSIREFLASEAMFG
                     LGVPTTRALSLVATGDRVLRDMFYDGNAREEPGAIVCRVAPSFVRFGNFEIFSSRGDL
                     ALLRRLVDYTIDALFPEIARTTPGLEKYAMWFAEVCERTALLVAHWQRVGFVHGVMNT
                     DNMSILGLTLDYGPFGFLDHFQADFTPNTSDPGRYRFMKQPMVAKWNLLKLAEALFPL
                     IESARPLEAGLTRFSEAFEAHQRRMQAEKLGLPDADLFDDLLEVLGAVETDFTLFFRG
                     LARVSTRSLQEDEELLAPLQNAFYDFPEMQGNDRQRVLAWLRRYVAAAQLQGLPDPER
                     RERMDRVNPKYILRNYQAQLASDAAEQGDFQPTRELLEVLSAPYDEQPEHESYAARRP
                     AWAKDRAGCSRLSCSS"
BASE COUNT         2344 a         4333 c         3989 g         2161 t
ORIGIN      
        1 atcacgcctc gcaacgggaa ctctgcagga attgccgcga tcagacgcgc caaagacgct
       61 gcatccgcga tatcgatcgc agccacctcc acctcggctc cgatgcgctg cagctccgca
      121 agggcctcgg ttgtgccggg agtttcggcg ccgcggcgac ccaccaggag caggcggcgc
      181 gcgccccgtt ctgcgcagcc acgcgcgagc atcaaaccca aggcgcccaa gccgccggta
      241 atcagaacgg tgcccgacgc cgagagtacg gaggctcgcg aatcagttga cgggcgatgc
      301 cccggcaccg tcgagagccg cgctacgtaa cgtcgtccgc gacaataccg cagttcgtct
      361 tcttcgtcct gggccgcgag ctccgcgttc aggatgcggg ccacgctggc cgcaaactct
      421 ggctcgttag cgtcgcagtc gatcagcttg cagttgagct cggggtgctc ctgccgaagc
      481 gttcgcccga cgccccatac cgtcgcggcc gcgggtgaaa ccacggcggg ctccgaaact
      541 ccgtccgtga gctccgaaaa aacgcgtctg gtgatccacc acaatcgagt cggtcgattg
      601 cggcgcgtga gcgcctgtac aatctggagg gcctgaatcg cggctcgggt ggcggcggtg
      661 gcatcctcgc cgacgtccca aacgcaaacc acgtgttcga ccgcgcttgc ttcgagggcc
      721 gccaaatcca aatgatcgac ggcagcgcca aggtcgctca aggcttcgcc gagccgctgc
      781 aggttggacc gctcgtgcag attcgctgac tcgtgcagat cacccgaaga gtgcggcccg
      841 agcgccacga tcccccaacg gctggcttgg cgttgttggc tgagcggggc cgtcgatttt
      901 tcgggtgtgt cgatccctcg cagctcggct tcgcgccagc tcaatcgata tagctcgtgc
      961 cgattgggcg ttaccttgga cgagctcgcc cgttcgatca atcgcggtac gtccgctcgc
     1021 cgcgcatgca gcgcatcgat ctcggcgatc acctcacccg tcggttcaca caaagtcaca
     1081 tcgaccacga ggtgctcggg actcgcatcg atcagctcca catggacgag cgcgttatct
     1141 gcggcggcgc gctgcacgcg gtagcgctcg catccgaacg gtagaaacgc ctgccccgtg
     1201 gcctcgagcg ggagcagcgc cgcgctcgcc tgcagcgccg cgtccagtaa cgcggggtgc
     1261 acgccgtagc cttgcgcgct caaggagctg tggagctcca gctccgcgat cacttcacgt
     1321 tcgccgcgcg acacgtacct gagcccacgg aaagcggaac cgtactgaat gcctcccgta
     1381 aggaaaccgg cgtgcaggcg ctccacatcc acgctcgtgc tgcagcgggc cctcaaagta
     1441 ccgacgggca ccttcggcag cgccgcgcca gaaacgtcag cgctgaccgg aatcgctcga
     1501 gccgtggcgt gccgcgtcca cgccgtgccc gtcaccgcat ccgcgggctg actgcacagg
     1561 gttgcgctca gcgcatccga gtctgaatcg cggctcaaca ccagctgaag ccttgttgtt
     1621 tgccccgcgc gcagcaccaa cggtgaagcg agcacgagtt cgctcacctc gcacgaaagc
     1681 ccgtcgtgcg cgtcttcggc ggacgctcgc accagctcga ggatggccgc ggccggcaac
     1741 actgcctccc cgccgacgcg atggtcgacg agccagcctg gttcgcgctc ggagatcaag
     1801 gtctcgtaaa cggcgaaggc ggccgcggta gccacccgaa cgcccaacaa aggatgcccg
     1861 ctctcgggtg cgctgcccgc caaatgaact ggtgcttcga gccagtagtt ctcgcgctgc
     1921 cagggatatg tcggcagctc gatgcgccgc agtccgttct tgcccccaat cattccattc
     1981 gggaagagcc gctcgggttg cagcggcatc ccgtgggtgt acagcgcgcc caaagcatcg
     2041 agtaacgcca accgctcggg ttgctctcgg cgcagtgatc cgacggcggt gcccgccact
     2101 cccgcttgcc ctcgaagctc ctcgattgcc gagagcaaaa gcggatgtgg gctcagctcg
     2161 acgaacaagc ggaagccttg ctcgatcaac ccttccacgg cgtccgcgaa acgcactggt
     2221 ttgcgcagat tgtcggccca gtatcgggcc gtctgctcgc tgccgctcag cgcacgcccg
     2281 gtcacggtcg agtagatggg aatcgtctcg gtcacaggag ccaagtcggt aagctgggag
     2341 acgagctccg cgagcaatgg atcgacctgc gggctgtggc tcgcgacatc gactttgacg
     2401 cgtcggcaga acaccccgcg ggcctcgagt gtggccaaga cgctcgctaa agctgcgggc
     2461 tcccccgaga gaacggtgga gtggcgcgcg ttactgaccg cgacgccaag ccgatcttcg
     2521 aagccacgca atgcggcttt ggcctcgtcg atcgagagct cgaccagcgc catctcgcct
     2581 ttaccgctga ttcgtcgcag cagcttgctg cgccggcaaa tcactcgcgc gccatcctcg
     2641 agcgagaggg agccggccac gcacgccgcg gccgcttcgc ccatgctgtg gcctacgacc
     2701 acgtcgggtt ctacgcccca cgatcgccag agggccgcga gcgaaacttg catggcgaag
     2761 agcagcggct gcacgacttc gatgcgatcg aattgcgcgc cactctcgag ctccgtcagc
     2821 acggaccagc ccgtctctcg gctgatggcc gcgtcacacg cctgcatcgc ctcccgaaat
     2881 accggctcct cgagcagcaa acttcgcccc atacccggcc attgcgagcc ctggccgggg
     2941 aagatgaagg ccaccttggg acgtgagatc gagtcagtcg tcgtaaccgg cgcgttttcg
     3001 gccgcaaggg cggcctcgag tgcggcctcg aacgcggcac gcgaagaaac ggcgaacgcc
     3061 caacgccgat ccatggctgc gcgggccgtc atcgagctga acgcgagctc cgccagggac
     3121 aactctggcc gtgccgcgag ctgcgacgag agctctcggg ccaaagcttt caaagcgggc
     3181 tcggtccggg cggagagcac gacgagctct gccgatcgcg ctaccgcgtg gggccccgaa
     3241 gcgatcgagc tcaccgcctc gagcaccacg tgcgcgttgg tcccgctaat cccgaaggag
     3301 ctgactcccg caaacgcgcc gccctggccg cgcgggaatg cctcgttctg cgtcgccacc
     3361 cgaaccggca gctgcgccca gggaatcttc ggattgggtt tgtgaaaatg aaggtgcggg
     3421 gcgagctgcc cgtgctcgag gcaaagcgcg gttttgatca aggcggcaac acctgccgcc
     3481 gcttcgaggt gcccgaagtt ggtctttacc gagccgagca ccaacggttg ctccggcgcg
     3541 cgcgcggcac cgtacacacg actcagcgcg agaacctcga tcggatcgcc gagcagggta
     3601 ccggtgccgt gagcttcgac gtaacccacg tgagcgggat cgacgccggc gtccgtgagc
     3661 gcgctgcgca gcacggcctc ttgggccgcg ccattcggcg ccgtcagccc gttgctccga
     3721 ccgtcgtgat tgacggcgct accccgaatc acggcgtgga tgtgatcgcc cgcggcgcgc
     3781 gcagccgcca agcgtttgag cacgacgacc ccacagccct ccccgcgtcc ataaccatcg
     3841 gctgcggcgt cgaacgtctt gcaacgccca tctggcgcga gtgcctgcaa gcgacacagc
     3901 gcgacgctgg cctccggcga agacatcacg ttgacgccgc ccacgatcgc gagctcgcac
     3961 tctcccgagc gcaggctctg gcaggccaag tgcacggcca cgagcgaaga agagcacgcc
     4021 gtgtccagct gcaaaaccgg cccgtgtagg cccaagacgt aagcgatgcg ccccgcggcg
     4081 acgctgcgcg cgttgccgag ggcggtatac gcatcgatgc gctcgaggtg cccggtcggg
     4141 atgcagcggc gcgcgtaatc gtccaagctc aagccgaccc agactccggt tgcgctgccc
     4201 gccaagcgat cggcgggaat tcccgcgtcc tcgagcgctt cccacgtcac ctccagcaac
     4261 aaccgctgct gtggatcgag gcccgccgcc tcgcgcggag agatgccgaa gaactccggt
     4321 tcgaaccccg agacctgatc gaggaacccg ccgcgttgcg tgtaacactt gccgggcgcg
     4381 ttcggatcgg gatcgtagag tgcgcgtgca tcccagcgct cgaggggcac cggacaggtc
     4441 gcgtcgacgc cctgttcgag taacttccag aacgcccgcg gccctgctgc tccgccaggg
     4501 aatcggcagc cgatcccgac aatcgcgatc ggctccgtgc gtcgctttcg ctccgcctcg
     4561 agcgcgtagc gcgcctcttt caataggcgg agcaggcgtt cgtcggagct cttcatgagc
     4621 gcgacctgag ctctcgctcc aggcgcgcca tctcgtcatc cagcctcacc tctaccggca
     4681 aggaggcgga aggccgctct gcttcgaagt tcggagtcac tggcggagga gggggcggcg
     4741 gctccgagcg ggagacccga gggggcccca ccaagtgctc gacgagcgcg tcgatggtcg
     4801 agaagttcca aagtacggtc tcgtcgagct tgcggcccac gcgatcggac agtgccttgg
     4861 cgagctccac cgcggcgatc gagtccaacc catggtccgc gaaggaacga ccggtctcga
     4921 catctgcgac cggaatctgc aggtgccgcg cgagccatga aaccacccag cctttgagct
     4981 cgcgcagcgc aggttcacgc gccgcggtag cctcgctggt gggtgccaca ctcggcagcg
     5041 gctcggattc gggggggcgc ttccaggccc caatgcgata gacgtccggt tcggcgcgag
     5101 ggtcgggatg cagctccccg atcgtgctgg cgtacgccgc aaaagtcgcg gcgacgtcgg
     5161 agtagtcgag catcgccgtc tcggacggcg ttcccagccg tacggcggag agagcttgct
     5221 ggaactgcgc gtccgcccag gcgtaagcgc gttccaaatc ggggctcggc tcgagcttgc
     5281 gggcgccatc caccgccgcg agcagcacga tccacgacgt tagttctccc gcgcgcgtgt
     5341 gaccccagcg ctgcgcgcgt cgcgccagtg ctccgctcaa gttcttcatg cgctgccgaa
     5401 ccgcgtcgag cgccgtgtcg atgctccgag ttagggttgg ggcaccgaat acttcctcca
     5461 cgagctgacg cagcgtcgta tcaccggcca tgaccacggc ccccgtgagc tccgacaaag
     5521 cctcgggtgg cccgtcgaag ttgcgcagca agcctgcatc gcgatggagc gcggaaagcc
     5581 ggtacggctc accacccgcc cccactcgca ttacatcgtc gatgcaacgc aagagcaggt
     5641 ctggacccaa aaccttgcac gcggcgaacg cctcgggggg caccgcaaaa ccttggtcgg
     5701 tggcctgtgc gacccggcgc accaggcact cgagcgcggt gatgctcgcg gtgaccgatc
     5761 ccaagcgcga gagcgtaatt gggtttggcg taaggcgtcc gttgatgccg ccgtgcagcg
     5821 gagtctcccg cccggcgagc tgggcacagc gtttcatccc gccgatgcac gcggcggcga
     5881 tggccaagcg cgtctgcatc atggcttcgc gagcgatatc caggcccgat ccgaagtttc
     5941 ccagcaaact ctcgcggcca acgagtaccc cgtcgagcac gacggtgtcg cgggcgaaac
     6001 cgagcagccc catcgagagg ccgtcattga cgtgcctcaa cccatccagc cccgaggaca
     6061 ccacgaacgc gctgatgccc ggaggctcat cgtggtgcgc aaacacgttc acgatgttgg
     6121 cgccctgcgc gaagccatcc aagtacttcg tcccgaacag gcgccaggca tcctcaccct
     6181 cggcttgggc ttgcatccca actccaccgg gcggagtgcg ccctgtcggc tcctcgaacg
     6241 cgaaacccgc gatctcctgg ccgtgagcga gccccggcag cagcaaagac ttgacctgta
     6301 cccgcgcata cttcgcgatc ggttgaatgc cgaggtagtt gttcaagccg acgaacagag
     6361 ccaaagcgaa atcgatcgcg gcgagttgct cgagcactcg catggtgtct acgtttccaa
     6421 gcccgaggcc gccgtactct ggttccacct gcatgccgag caggccctgc ttggccaaat
     6481 cccgaagtag cgcgctgggc gtcgaggaac gcggagcgtt cgaataggca ttgatcagat
     6541 cggcggcgtg tcgccgcaac caatcgatga gcgcatccgc tttgacggcc cgcgcctgtc
     6601 gctctccgtc ggcatgcgca gcggcgtctt gcggcaaggg cgacgtccac gccaccacgg
     6661 aaggcagcgc gttgctcaga aacgcctcgc gacaggcctt tcgccgtact ttcccgctcg
     6721 tcgtacgcgg caaggtcgag ggcttgagta gcacgatggc gtgagtatgc agcgcgtgct
     6781 gctcggagac ggcgcgccgg atggcacgaa ccaccgcggc cgcatccaac gtccgcaacg
     6841 ccgtgcgcgc cacctcttgc accacgacga gctgctcgcc ctggttggtc tcgatcgaga
     6901 acgccgcgca cgcgtggggc accaaatcgg ggtggctcgt cgaaacgctg gactcgagat
     6961 cgtggggata gtgattacga ccccgaatga tgatcaggtc cttccgacgg cccgtcacga
     7021 acaggcgatc gtcgagaacg aaacccaagt ctccagtcct gaggaacggc ccctcaccat
     7081 ctgcccgtgt ggcgccgaac gtttcgcggc tctcgcgcgg cttgccccaa tagccttgag
     7141 ccacactcac gccgcgcacc catatctcac ccacgcggcc cggggcgcaa ctgtggagac
     7201 tttccggatc cacgatgcaa agctcggtgc cggcccaagc gtgcccgcac cccaccaaac
     7261 tcgagctctc tttcgaagat ttcaccgcac gtccgagctc cagctcacgc gcgtcgaagt
     7321 gctcgagaac gggcggctgc gacgcaggct cgccggtcac gatcaaggtg ccttccgcca
     7381 acccgtacac cgggtgaaag gcctcgaagc gaaaaccagt gctcgcgaac gcttcggcga
     7441 aacgctgcaa ggtcgcggcc cgcaccggtt cactgccgtt gtacgccact cgccagcagg
     7501 agaggtctag cccggcgcgc tcctcggggc ggatgcgccg cacgcacaag tcataggcga
     7561 aatccggccc tccgctgctc gccgcacggt actggctgat tgcgcgcagc catcgcaccg
     7621 gactctgcag gaaagactgc ggagacatca aaacacagcg cgccccgagc ctcagcgtct
     7681 gcagcactgt tccgaggccc atgtcgtgga acatcggcaa ccacgagacg gcgcagcgat
     7741 ccggatcgtg ccggaagatt tcgtcgatct ggatttgatt ttgaaccagg ttgccgtgcg
     7801 taacggccac gcctcgaggc gccccggtgg agcccgacgt atattgcaga agcgccagct
     7861 cctcggggcc aacacgaggc agtgtggttg gctgcgaaac tccagtcacc gcgcacgcct
     7921 cggtatccaa gaacgtcgga gcgtaagcgc tcggtgtctc ggccagctcg cgcgtgattt
     7981 gctgcagcag atcccccgtc gacagcagcc atttggcccc agcatccgcg gccacccgcc
     8041 tcagcccctc gaagccgcgc tttcgactcg gcaagctgac cggcaccgcg atcacgcccg
     8101 cgtagagaca cccgaaaaac gcggagatga actccaaacc ttgcgggtac agcagcaacg
     8161 cgcgctctcc ccgcgcgccc aaccagctga gccgatgcgc gatcgcgagt gcggcctggt
     8221 gcagctcccc aaaagttaga ctcttggcct cgacttcgcc ttcatgaaga aaggtatacg
     8281 caacgcgatc cggttggcct tcggcgcgtg cctgcaggac ttgtacgaat gttgcgggta
     8341 cggagcccgg cgctgaagtt gccaagtctt ccgactatac gcggcccgtc cactcaatca
     8401 accggatccc gagccgtctg acatttcacg cacgcgcaga tctaagcttg gtcatctcgc
     8461 gtgttgccga ggcatgtcct cggttacata tctcacggtc ggagcgctca tgtccgtcgg
     8521 tcgatttcga cggctcgagg ccctctgcgt aacgacttcc tctcggaaat cacttgtggg
     8581 ccacagccac gagcgtaacg ccagccgtgt tggatgacgg caatctgact aatctccgcg
     8641 aatcccgcag ccaggccttc cgagcgccca ctcgaacccc gagtttcgtt cgtctgtccg
     8701 ggaggttcta tgccccgagg gtaaatgctt cgattgggct ggtcagcaat ctgacgcaaa
     8761 aactctcatg acgcggtgaa gcttccttcc tagccgcgct cccgcggtga cccgtggtct
     8821 ttcaaccggc gtagcgccca cgctcggctc gtgcgggtcc tcgcttcagc cgcctcagaa
     8881 tgagccctga cagccgatgc tgagcagccc aacaccacaa gtggtagagc gggcgaggga
     8941 atccgaggcg gagccggacg cgctgctcga cgtggacgaa cgcgtgctca accaatgaat
     9001 caaagcacca gtacccagta ctacggccgc ggcgccgccg acgatcgcga cgcgctcggc
     9061 ggtttcggcc gacttccgct catccccgca agtctcttcg cgagtcttcc ccgtcgtagg
     9121 caaacatcga ttgtcgtcgt tccaacgttt ggcgtgctgt tcgcgaatcg cgaacgcgac
     9181 gcctcccgtc gcggcggcgg ccaagcccaa tccggccagc gtccacgtaa accagcgacc
     9241 agaactcgtt ggctctacct gccgtgcggg agctcgttcc ggctgaatcg gggcactgct
     9301 cgggctctcc atctgcacca agggtgaaga cacgtctcca ccggcctgag cggaggcggg
     9361 cgcggaggcc gcggcgggcg gtaactcgtt caggtcgatc gactctcgag ccagaattcc
     9421 ggcctgaatc gtaatctgtc ggcgaacccg gtaatagccg ggctgggaaa cctcgagcac
     9481 gtaggtaccg gcctcgacgg tctgaggctc ggccatgttc aggttcccga tcagctcgcc
     9541 attgagccga atttctgcgc cgagcggggt tccagacgcc gtgaatcggc caaccttgcg
     9601 agccagagta ttttcgatgg ccgtgatcgc ggctcgatga cgctgatagt aaggatcgtc
     9661 ccggtgtgaa ctcaccttgc gaaagtatgc gtcgcactcg atccacttcc cgactgccgc
     9721 cgccgcgccc gcaatgttca ggagcacgcg taccgagtct ggattgcgac gttcggcgtc
     9781 gtgaaatacc tgcagggctg cctcgtcgtt ccccgctttg cgcagccgaa ttccctgttg
     9841 gatcaagtcc tccagcgttc gatcctgcga ttcggaaatg ggtacactcg gatccccctc
     9901 cgcgaatgcc agcgacggtg cgagcgtcgc agccagaaga gtccaagcgg tgaaacgaag
     9961 cgataaagca gagcgaagct tcatggagaa gaatcggttc gaaccctagt accccgcttg
    10021 ctcgaagcca aggccttcct ggtgcgtcac ccaggagcaa caaattgacc acactggact
    10081 tgcggcttgg ggcaaaccgc tggattggcg gcaggacggg gaagccgccg aatcgcggaa
    10141 ttcagttgcc taaactacct ccgccgaagc gcgcaccagc gcatgtagcg gaggaggcgc
    10201 gaccgttccc ctcgtcctcg gctccactat cgttgccgag ctctaaccgg ctccaccggc
    10261 gcggagcgcg ggcgagacga gcgccggggg tcgataaccg cctcccgtgt cgcgaatcag
    10321 acatcgaaca ccggacgctg agtcccgtcc gtttcccggc gctgagtcga gcacgtttcg
    10381 gccggtcttt gacgcgcccg ccctggaaaa gtccttcgtc aggcgcggac gctttcggtg
    10441 gcacgagcat tgcccaagtc gcgggccgtg tccgtgatgg ctgggcgcat cccggcgttc
    10501 gccaatcgtc atcctcaggg agtcagaaat ctacaggagt cagtgacatg gatcgagaat
    10561 cgagaggtca ttccgcagcc gtcaccgacg gggttcgggt cacggtcgag gctcgctaca
    10621 gcgccgagca atccctaccc gcagcgcgtc gctacgtgtt cgcctataca gtgcgaatcc
    10681 gaaacgaggg gcgcgaaggc gtgcagctgc gcagccggca ctggatcctc acggatagcg
    10741 cgggccacac cgaagaggtg cgtggggaag gcgtcattgg cgaacagccg gtgctacctc
    10801 caggcggtca attcgaatac atgagcgccg ccgttctcaa gacgcctcgc ggagagatgc
    10861 gcggcagcta ccagatggtg acctggaatg ggcgcagctt cgacgcaacc atcgcgccat
    10921 ttcaactcgc cctaccctac tcactgaact gagcgcttac ccaataggat ggatgccgga
    10981 gtcgtgagga gcggggccac agctgcgcgg agagagaacc agctgcgcgg agagagaacc
    11041 agttgcacgg agagagaaat gggccaagag caccaggcca tgtcttcctc gctcgagctc
    11101 cctgccttca agaaccgctt cgttacggag cttccggggg aagcagatgc ctcgcgccac
    11161 tcgcgcgcgg tccatggtgc ctgctattca ctcgtacgcc ccgtgccagt ctctgcgcct
    11221 agcctggtgg cttactctcg ggatctagcg gagcggctcc ggttggacgg gggaacgctc
    11281 gagtccagcc tctggctggg catcctctcg gggaacaccg tgtcagccga catgcagccg
    11341 tacgcctgct gttacggggg ccaccagttc ggcacttggg cagggcaact gggggacggg
    11401 cgagccatca cgctgggtga gttggtcgac cgagagggtt gccaccaaga gctacaactc
    11461 aagggttctg ggcgaactcc gtattcccgg accggagacg ggcgcgccgt catgcgctcg
    11521 tccatccgag aattcctagc tagcgaggca atgtttgggc taggcgttcc tactacgcgc
    11581 gcgctcagcc tagtggcgac gggcgatcgg gtgctgagag acatgttcta tgacgggaac
    11641 gcacgtgaag aaccgggcgc catcgtgtgc cgcgttgccc cttcgttcgt gcgctttggc
    11701 aatttcgaga ttttcagttc acggggagat ctcgccctcc tgcgccgcct cgtcgactac
    11761 accatcgacg ctctgttccc cgaaattgcg cgcaccacgc cggggctcga gaagtatgcg
    11821 atgtggtttg ccgaggtctg cgagcgcacc gcgctcctgg tcgcgcactg gcaacgagtc
    11881 ggcttcgttc atggcgtgat gaacacggac aacatgtcga ttttggggct cacgctcgac
    11941 tacggaccat tcgggttcct cgaccacttt caagcggact tcacgcccaa cacctcggac
    12001 cccggacgct atcgcttcat gaagcagccg atggttgcca agtggaatct cttgaagctc
    12061 gccgaagcgc tcttcccgtt gatcgagagc gcccgcccac tcgaggccgg gctgacgcgc
    12121 ttcagtgaag cgttcgaggc ccaccaacgt cgcatgcaag cagagaagct tggcttgccg
    12181 gatgcggact tgttcgatga cctgctggag gttctcggcg cggtcgaaac cgacttcaca
    12241 ctcttcttcc gtggactggc gcgcgtctcc actcgtagct tgcaggagga tgaggagttg
    12301 ctcgctccgt tgcagaacgc gttctatgac tttccggaga tgcaaggcaa cgaccgacag
    12361 cgtgtcttgg cctggttgcg ccggtacgtc gcagcggctc agctacaagg gcttcccgac
    12421 ccagaacggc gcgaacgcat ggatcgggtg aaccccaagt acatcctgcg aaactaccaa
    12481 gcccagctgg cgagcgatgc cgctgagcag ggagatttcc agcctacacg cgagctcctg
    12541 gaggtgctga gcgccccgta cgacgaacag cccgaacacg aatcgtacgc ggcgcgacga
    12601 ccagcctggg ccaaggatcg cgccggctgc tcacgcttgt cttgcagtag ttgaggcgtt
    12661 ggattcggtt cactcggccc gaatccagcg ggccgcggct cattcgcgca gaggggccac
    12721 tcgagccggg attggatgct ctccccacgc gctcgtgccg ttgagcttct gcttcccctt
    12781 catttccagc cctctgaaca tcgagcaccg ctcgaaaacc tccaacg
//
LOCUS       DASTKU010000280         3641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_276592, whole genome shotgun sequence.
ACCESSION   DASTKU010000280 DASTKU010000000
VERSION     DASTKU010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276592"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VFQ61_10090"
     CDS             complement(<1..405)
                     /locus_tag="VFQ61_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954845.1"
                     /translation="MAAAFAAAIGMAALWGFTVDDAWISARVASNLARGAGYRFNSDG
                     PVVDAVTPLGWAPLLALFAARGSTAALLAARWIGALTWLVAAAGLGVKWSRLGLRAAI
                     TGTTAIALTVPLGAWAVSGMETGLVIALGTVAL"
     gene            630..2918
                     /locus_tag="VFQ61_10095"
     CDS             630..2918
                     /locus_tag="VFQ61_10095"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9954846.1"
                     /translation="MSAIEVVVRPRSQAPQPENPSALRGLFDILVDGLSLSTRLGEGQ
                     SLTLLCDLAQAVSGLGRGKKSRATLPIYTEDEAWELGLESDATEVLLSVYRVGPRPEV
                     LLHERRLDAAVLRTTLLRAMDEAIAQSANRTATAALSGARSLLTGLIGHSERRAAPRR
                     LCRVEPKAVGTLAFSAEAPFRLGETQDGGALAASPHVERADLHALLVRGSAFATARGR
                     TLTIEGVYPFLIAEQLLALAEDVLEAWQAERSLVRLVTVDGARLGVQRSGAGGLTLTF
                     RPVQGARAAGGVALPELTPQRFIKAAVRFARALAEQFISLDPIQSQNLRLLSLVREAG
                     ELETAARDAALDDSLTNPEPEAYKTFGLPRPPAERRLWANGGKMRFLPRWAATVPGLD
                     LRATFHCGSRIVVGGQRETACLESQSGRVLWRIPGSRAACVATPVGIARVHQDGLVEL
                     FDLDRGEMRVATRLRPRATSGAAGALVNAPGLPRLLVLAEGDRSVTALDLVSGEVRWR
                     FSARRPTQYRLRRAGRLLLAAGGDTALIALDVVTGEVVWRVRDRRAFTGELCVDKDSA
                     FALVGGPIGSCKLLHVDLWSGAVRFERELEDRPLAGQAPLITANRVMLPIRDRRGLGV
                     VALERESGVPCWRQEPGFTSGTTSFLAVDDLLVANTASGAFLALEAETGHLRYNHVFS
                     RNVDSDQPRRLEPVLRNGALFVPQHRVHVVRPRDGEVIGCVPSDLIPDLLRVDECCTV
                     FIAEESGHLAAFGVAPKLELVR"
     gene            complement(2929..3384)
                     /locus_tag="VFQ61_10100"
     CDS             complement(2929..3384)
                     /locus_tag="VFQ61_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954847.1"
                     /translation="MGQFKAFLLLAISLVITLARRLFSRRGIGITAFRENYDADGLPP
                     VSAEERRELTSFQRCIACGLCDRGEGQRIAASRGSYRGVMSLMLAGSRSMPDFRASAH
                     AFSFVPESVLAEKEALCPTQVPMRQIARFVVAKAAAVGGPLPTMVPPDG"
BASE COUNT          621 a         1165 c         1201 g          654 t
ORIGIN      
        1 gagcgcgacg gtgcctagcg caatcacgag ccctgtctcc attccagaga cggcccacgc
       61 accgagcggc accgtcaacg caattgccgt cgttccagtg attgcggcgc gcaggcctag
      121 ccgcgaccat ttgaccccca accccgcggc cgcgacgagc catgtcagcg cgccaatcca
      181 acgggccgcc agcaaggccg cggtagaacc cctggctgcg aacagcgcga gaagcggcgc
      241 ccagccgagt ggcgttaccg cgtccacaac cgggccgtcg ctgttgaatc gatagcccgc
      301 tccccgagcc aagttcgaag cgacgcgagc gctgatccaa gcgtcgtcca ctgtgaagcc
      361 ccacagcgcc gccatgccga tggccgccgc gaatgctgcg gccacggccc ccgcggcgaa
      421 aaccgtcagt tgcggttttg ttgacctttg cggagtgctt tcgaagaaca agattcgtcc
      481 gaaaatcctg gggcagcgta gatgacgccg cgggctttgg cattgcgagc aaggggctac
      541 gccgacccgg gtttccctgt ccagtcttga gccaaagatg ccgagaagta gggctttatc
      601 cgccgtttac ctacgatgta cggcgtccga tgtcagccat cgaggttgtt gttcgtcccc
      661 gctcccaagc cccccagccg gagaaccctt ctgcgcttcg tgggcttttc gatatcttgg
      721 tcgacgggct gagcctgtcc acccgcctgg gtgaaggcca aagcctgact ctgctgtgcg
      781 acctggctca ggccgtcagc gggcttgggc gcggcaagaa gagccgcgcc acgttgccta
      841 tctataccga agacgaagcg tgggagctcg ggctcgagag cgacgcgacc gaggtcctgc
      901 tgagcgtgta tcgggtcggg ccacgccccg aagttctgct ccacgagcgt cgcctcgatg
      961 ccgccgtcct gcgcaccacg ctactccgcg cgatggacga agcgatcgcg caaagcgcga
     1021 atcgtaccgc gaccgcggcc ttgagtggtg cgcgtagcct gctcacgggg ttgatcgggc
     1081 attccgagcg gcgggcggcg ccgagacgtc tttgccgagt ggagcccaaa gcggtcggga
     1141 cgttggcctt ctcggccgag gcacccttcc ggctcggcga aactcaggat ggcggggcgc
     1201 tcgccgcttc tcctcacgtg gaacgcgcgg acctccatgc attgctggta cgcggcagcg
     1261 cgttcgcgac cgcgcgcggt cggacgttga ccatcgaggg cgtctacccc tttttgatcg
     1321 cagagcagct gctcgcgctg gccgaggacg tactcgaagc gtggcaagcg gaacgctcct
     1381 tggtgcggct cgtcaccgtg gacggcgcgc ggctcggcgt tcaacgctct ggggccggtg
     1441 gcttgacgct gacgtttcgc ccggtgcaag gagcgcgagc cgcgggcggg gtcgcgctgc
     1501 ccgaacttac tccgcagcga ttcatcaaag ccgcggtgcg ctttgcccga gctctggccg
     1561 agcaattcat ctcgctcgac ccaatacaga gtcagaactt gcgcttgctc tcgttggttc
     1621 gagaagcagg ggagctcgaa accgcggccc gcgatgcggc cttggacgat tcgttgacca
     1681 acccggaacc cgaagcgtac aagacgttcg ggctcccacg cccgcctgcc gaacggcggc
     1741 tttgggccaa cggcggcaag atgcgttttc tgccgcgctg ggccgcaacc gtgcccggtc
     1801 tcgacttgcg ggcgacgttt cattgcggat cgcgcatcgt cgtgggtggc cagcgcgaga
     1861 ccgcgtgcct cgagagtcaa agcgggcggg tgttgtggcg aattccggga agtcgcgcgg
     1921 catgcgtggc taccccggtc ggcatcgcgc gagtgcatca ggatgggctc gtcgagctgt
     1981 tcgatttgga tcgcggcgag atgcgagtag caacgcggct gcgcccgcgt gccacgagcg
     2041 gggccgcggg ggcgctggtc aatgcaccgg ggttgcctcg tctattggtt ctggccgaag
     2101 gagaccggtc cgtcaccgcg ctcgatctcg tttccggcga ggtgcgctgg cggttcagcg
     2161 cccgccggcc cacccagtat cgtctgcgac gcgccggtcg tttgctgctc gccgccggtg
     2221 gcgacacggc gttgatagcg ctcgacgtgg tgaccggaga agtggtttgg cgggtccgag
     2281 atcggcgcgc cttcaccggt gagctttgcg tcgacaagga cagcgcgttc gctctggtcg
     2341 gcggccccat cggcagctgc aagctgttgc acgtggattt gtggagcgga gcggtgcggt
     2401 tcgaacgaga actggaagat cgaccgcttg ccggacaggc cccgctgatc accgccaatc
     2461 gggtgatgct accgattcgg gatcggcgag ggctaggcgt ggtcgctttg gagcgcgaga
     2521 gcggcgtgcc ctgttggcgt caagagcctg gctttacgag cggaacgact tcgttcttgg
     2581 ccgtggatga cctgctggtg gcgaatacgg cgtccggagc ctttctcgcg ctcgaggccg
     2641 agacaggtca ccttcgctat aaccatgtct tttcccgcaa cgtcgattcc gatcagccac
     2701 gacggctgga gccggttcta cgcaacggcg cgttgttcgt tccgcaacac cgtgtgcacg
     2761 tggtccgccc tcgagacggg gaagtgatcg gttgcgtccc gagcgatctg atcccggatc
     2821 tgctgcgagt cgacgagtgc tgcacggtgt tcatcgcgga agagagcggg cacctcgctg
     2881 cgttcggggt cgccccaaag ctcgagctcg ttcgctgacc taataaactc aaccgtctgg
     2941 tggcaccatc gttggcagtg gccctcctac ggccgctgcc ttggccacga cgaagcgggc
     3001 gatctggcgc atcggaacct gggtcggaca gagagcttcc ttttctgcca agaccgactc
     3061 cggaacgaag gaaaaggcat gagcggaagc tctgaagtcc ggcatgctcc gcgagcctgc
     3121 cagcatcaag ctcatcactc cgcggtagct gcctcgagag gcggctatgc gctgcccctc
     3181 tccgcggtca cacagtccgc acgcgataca gcgttggaag gatgtcagtt cccgacgctc
     3241 ctcggcagag accggaggca aaccatcagc gtcgtagttc tcccggaatg ccgtgattcc
     3301 gatacctcgt cggctgaaca ggcgccgtgc gagcgtaatc acgagtgaga tggccaggag
     3361 cagaaacgct ttgaactgac ccattgaggc tctgtctatc cgagctaccg atccgaaacg
     3421 cgcgaactag gcgtagcgct ggtgctgtcc aagcatactg gccaagtgtg ctggccaagc
     3481 atactgacca aaactgggga acggactcga attgaagata gcctaagggg tggagtccgc
     3541 caccggcaag ccaagagcgg ctcgcgccag gtcgcgcccc aaattctcac ctgctgcgcg
     3601 caacgcaccc gtttggcgta cccaatccgc ggactcgcgc t
//
LOCUS       DASTKU010000281        10917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_55553,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000281 DASTKU010000000
VERSION     DASTKU010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10917
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55553"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VFQ61_10105"
     CDS             <1..1021
                     /locus_tag="VFQ61_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954848.1"
                     /translation="SRMLSGVLSVCVFGIGCARNEVVSLHPPSSPLQLARAGLYPETL
                     AYDPAGSRFLVGSFREGAIYEVHADGHVTSVVDDPRLCSVLGIAVDDRRGRLWAVNSD
                     LGACVRASPVGPKNLAAVGVYDLRSGAPLQYVDLTQALPSLPTGPHLLNGLALDGAGN
                     AYVSDSFSPAIYRIDAAGNPELFLQTPEFAGEGVNLNGLVVHPAGYLLVIKKSDGALF
                     KVPLQDPERYARVSLSEPLFGGDGLVLVDDRNLVVVSNQTPQHASNSALVLRSTDDWA
                     RASVVARQALGDVYPTTAALRGADLYVIHSKLNELIQAPPDQKSKYNAFATIQQIGHV
                     QHAAW"
     gene            complement(1057..3315)
                     /locus_tag="VFQ61_10110"
     CDS             complement(1057..3315)
                     /locus_tag="VFQ61_10110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HET9954849.1"
                     /translation="MKRKLPGLKAGPRSSSSKRGNSEVWVGKLNMNPRGFGFVAEPGK
                     GDAYISPDSIGGALHGDRVEIEIVGRDSRGAEGRIRQILERRPARVGGVLRRRGKSAW
                     LEPDDSRIRGPIVIAANSKQGRDGEAAVVSITRFPEFANENPEAELLDVLGEPGDPQV
                     EVAKILLREGVEEAHPEPAVQAAESMFARLVRPGADGRRDLRHVPLPTIDPEDARDHD
                     DAVWVERRGEGYRAYIAIADVSAYVEPGSALDDEASLRGCTIYLPDRAIPMLPSALAA
                     DLCSLLPERERLCLCVIADLDGAGDVESFEIVEGIMRSAAMLTYGGVARALGFTEEPP
                     RSAQAEAMRKDLKVLDELARKLRKARLARGALDLDLPEPKLVLDPDSGTPLDVKRRTQ
                     DPGIRRAYSMIEELMLLANEVVARFLGERKSPAVYRIHAAPDPLKLERLQAIAEKLGL
                     AVDVDALREHGGVARFLAKIHKHPKKQVLESLLLRSLMQATYDTNNVGHYGLASEAYV
                     HFTSPIRRYPDILVHRAVKHLLAGGKPKTTPTALEALADQARTSSERERRALDIEREV
                     VDLYRTLLMRERVGDILEGSVAALVGSGAFVTLDDPFVEVLVRFESMGPDRYELSEDE
                     LSVVGIRSSDRINLGDRVRVKIEDVSILRRTVYAARVLSDEQLEKLADSPSAPTDSGA
                     DASTERRGRKRVFTVEANYAGKPSKSGGFRGASGRSGTATRPDRGGKQVKQDPRASRR
                     KREDRKRRHKQR"
     gene            3574..4677
                     /locus_tag="VFQ61_10115"
     CDS             3574..4677
                     /locus_tag="VFQ61_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9954850.1"
                     /translation="MAPGKALLLLRSGLLVALAGCAALTIEYRSSNPSFCSVNSGCAA
                     LRRTPLAYLWGAGITLPELGLIAVSLVFALSLTQKPRIAAILTLVAGAVGALLIVAQA
                     LFLNAFCWLCMTVDGSSLLAALGAAAWLRARKDEAPPTYGLKTWAWAALAGVAVVAPV
                     AWPSVKPAPPVPPSILAQYVAGKINVIEFADFECPWCRSLHARLKPLLAEYGERVHFV
                     RLNMPLERHPSAKGAALAAICSEPSGKAPALAEFMFTTEDLSPENIRREVANLGLDLA
                     EFDRCLAAPATQQRLTREMDILKQAGFEGLPTTYVGSRRLIGAQPDEVFRNALDRAAR
                     GDGNRGVPGWGYLLIVLGVVASILRFGLERRTG"
     gene            complement(4777..4992)
                     /locus_tag="VFQ61_10120"
     CDS             complement(4777..4992)
                     /locus_tag="VFQ61_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954851.1"
                     /translation="MVHIRGTTYRVVRLKRGAYDVVRVLDDCRVGSFSLAPHFKTLPL
                     CIDDELMRAIARAAIRSGRTTWDPSGQ"
     gene            complement(5119..6276)
                     /locus_tag="VFQ61_10125"
     CDS             complement(5119..6276)
                     /locus_tag="VFQ61_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HET9954852.1"
                     /translation="MLERLQQGRVPAKPHTVFHSDSGGLLYEECVTRGGFEGAYTIVY
                     HQHRPHAARAIPAPCQFRVPRAEPERALLRRHYRCGEVSRAGEHSTLDGRVPLLFSRE
                     LVLGFVQPTQDDRVYFVNADGDELFYVHEGSGQLRSPQGDLTFNQGDYVLVPKGTLYR
                     VLLGASDRHALLWLESHVDFGIPPTFKNGAGQLRMDAPYSHRDFRTPEFKGPNDEGLR
                     DVVVKRGSRFFGFRFEHSPLDLVGWDGAVYPVVLPILAFQPRVGQIHLPPTAHMTFQS
                     RSLIVSSFVPRPLDFHESANPCPYPHSSVDVDEVLFYSAGSFLSRRGIGAGSLSHHPA
                     GIPHGPHPGAYESGPTKRSTDELAVMLDSSLPLEATPEAVSIEDAAYHESF"
     gene            complement(6276..7457)
                     /locus_tag="VFQ61_10130"
     CDS             complement(6276..7457)
                     /locus_tag="VFQ61_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9954853.1"
                     /translation="MARVESIGIRRLEAIHYYVHDLERSQRFYTDKLGFACTARSGAE
                     LVEGGHQRALVFESGNCRVVCSSPVGEGGRAHRYLAKHPDGVGSLIFEVENIERAFAL
                     LETRGGTPIHEIQETRTPTGGFKSFVIATPFGDTTFRFLERTGDFTLYPGFEALAAPV
                     SDVTAPFLTFDHVTSNFETMSPALLWLEHVLGFERFWHVEFHTDDVRQRVDKAGSGLR
                     SVVMWDPQSQVKFACNEPYRPNFKASQINVFHEQHRGDGVQHVALTVRDILSCVERLR
                     TRGVEFMPTPKAYYDALRARLTELGVGDIAEPVARLEELGILVDGAGRGRYLLQIFLK
                     DSAHTYGSEEAGPFFYELIQRAGDPGFGAGNFRALFESIEREQLSERDREPEATTKGA
                     S"
     gene            complement(7502..8473)
                     /locus_tag="VFQ61_10135"
     CDS             complement(7502..8473)
                     /locus_tag="VFQ61_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9954854.1"
                     /translation="MRFASLRDGSRDGALVLVDARHERCVSTAAIVGTLQEALDIWAE
                     CLPQFDALQDRLERDQVPSQPFDPQLCGAPLPRAYEWIDASTYLNHVELARRSRGAAL
                     PPDLQTSPLVYQGGSSVLSGPTEPLRLLDASFGLDFEAELAVVLSDVPLGTNAHSARD
                     RIQLMMLANDVTYRNLVPGELEKGFGFFQSKPATAFSPLAVTPDELGSAFRDSRAFLT
                     LRCELNGERVGELQTGPEMHFSFAELIAHISRTRAFCAGTILGSGTISNRDATRGVAC
                     LVERRMRERLESGQAVTPYLKTGDRVLIRAFAADGKDVFGQIDQTVI"
     gene            8596..10356
                     /locus_tag="VFQ61_10140"
     CDS             8596..10356
                     /locus_tag="VFQ61_10140"
                     /EC_number="1.14.16.-"
                     /inference="COORDINATES: protein motif:HMM:NF010657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0009072 - aromatic amino acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid hydroxylase"
                     /protein_id="HET9954855.1"
                     /translation="MSVSAPKVAPLQPAEAVPRHLRRFVVEQDYSQYNVVDQAVWRFV
                     LKHTHARLISTAHPAYHSGLSATGISLERIPSIEEMNERLARFGWGAVCVDGFLPPRA
                     FQSFQAERILPIAGEIRTREHLVYTPAPDIIHEASGHAPILTDPVFSRYVQRMGWLGR
                     QAFTLPSEERVYQAIYNLSEIKENPASDAAKVQQAEAELALAVKHAGEPSEAARLSRL
                     YWWTAEYGLIGRPEAYQIYGAGLLSSLWESYSCHAASVSKRVLDETCMDVAYDITRTQ
                     PVLFVVPSFEALHGLLDRVERGMAAQVGGRYALEHATRSAEPATFEFESGARLYGVLN
                     GFAGERTKRLQFGGSVRAVWRMGEPSIALGADLCIVAGRTLDGQGLAEWMVQGSHGAS
                     GRVQVQFAEGTSLVGRLAAQAVTGPDGAARCLQLEDVELRSPDSPPRFFREYTLLALG
                     EFVTARAGADSDLERELSLYERTLSTEQKTPSPWRVPRVRVFAEPELRLQALYSRWER
                     VRESAFAEQSMEIRALYAALAEYPNEWLLRLQLLQGATRMPELADLQRTLRGELERLE
                     LLFEGREPIETGLREVGHAA"
     gene            complement(10540..>10917)
                     /locus_tag="VFQ61_10145"
     CDS             complement(10540..>10917)
                     /locus_tag="VFQ61_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954856.1"
                     /translation="HPEPQKLLHLAVLESAKAPLDVPTSDHVFTLIGLATGQQNAELV
                     RQGLRSPDQKLRGTALEYLESLLPEGVRVAIVTALEHNTVERAEHSRPEVARPETELC
                     DELRRSLRGHEIPRRLEEDLDDT"
BASE COUNT         1890 a         3422 c         3568 g         2037 t
ORIGIN      
        1 ctcgcgcatg ctctcgggcg tgctctcggt atgcgtgttc ggcatcgggt gcgcgcgcaa
       61 cgaagtggtg agcctgcatc cgccgagttc accgctgcag ctcgcgcgag cggggctgta
      121 tccagaaacc cttgcctatg atcccgcggg ctctcggttt ctggtcggtt cgtttcgcga
      181 aggcgccatt tacgaagtgc acgcagacgg gcatgtcacg agcgtggtcg atgatccgcg
      241 cttgtgttcg gtgctcggca ttgcggtcga cgaccggcgt ggtcggttgt gggctgtcaa
      301 ttccgacttg ggcgcttgcg tcagagcctc gcccgtgggt ccgaagaacc tcgcggcggt
      361 tggagtctat gacttgcgga gcggcgcgcc cctccaatac gtcgatctca cgcaggcgct
      421 acccagcctg cccacgggcc ctcatctttt gaacggcttg gccttggacg gcgccggtaa
      481 cgcttacgtg agtgacagct tctctccagc tatctaccgg atcgatgcgg cgggaaaccc
      541 agagctcttc ctgcaaactc cagaattcgc aggcgaaggc gtgaatctga acgggctggt
      601 cgtacacccc gcaggttatc tgctggtgat caagaaaagt gacggggccc tattcaaggt
      661 cccgttgcag gatcccgagc ggtacgcgcg tgtgtcgttg tcggagccgc tgtttggagg
      721 cgatggcctg gttctggtgg acgaccgcaa cctggtcgtc gtctccaacc aaacgccaca
      781 gcacgcttcg aactcggcct tggttctgcg gagcacggac gattgggccc gggccagcgt
      841 ggtcgctcgc caagcccttg gcgacgtata ccccacgacg gccgcgctcc gaggagcgga
      901 cctctacgtg atccacagta agttgaacga gctaatccaa gcgccgcccg atcaaaaatc
      961 caagtacaac gccttcgcca caatccagca aatcgggcac gtgcagcacg cggcgtggta
     1021 accgcggccg cgcgggcccg agaggatttc ggcagctcag cgctgcttgt gacgtcgctt
     1081 gcggtcctca cgcttgcggc gtgaagctcg cgggtcttgc ttgacctgct tccctcccct
     1141 atcgggacgg gttgcagttc cgctgcggcc gctcgcgccg cggaatcccc cgctcttgga
     1201 gggtttgccc gcgtagttgg cctcgacggt gaagacgcgc ttgcgcccgc ggcgctcggt
     1261 gctggcgtcg gcgccggagt cggtcggggc gctcggagaa tcggccagct tttccagttg
     1321 ttcgtcggac aacacgcggg ccgcatagac cgtgcgccga aggatcgaga catcttcgat
     1381 cttcacgcgc acgcggtccc cgaggttgat gcgatcggac gagcggatgc ccaccacgct
     1441 cagctcgtcc tcgctcagct cgtagcgatc gggacccatg ctctcgaagc gcaccagcac
     1501 ctcgacgaac gggtcatcga gcgtgacgaa ggcgccgctg cctaccagcg ccgccaccga
     1561 gccttcgagg atgtcaccta cgcgctcgcg catcagcagc gttcggtaga ggtccaccac
     1621 ttcgcgctcg atgtcgagcg cgcgccgttc gcgttcactc gacgtgcgcg cttggtcggc
     1681 caaggcctcg agcgcggttg gcgtggtctt gggtttgccg cccgcgagca agtgcttgac
     1741 ggcgcggtga acgaggatat ccggataacg ccggatcggc gacgtgaagt gcacgtaagc
     1801 ttcggacgcc agcccgtagt gaccgacatt gttggtgtcg tacgtggcct gcatcagtga
     1861 gcgtagcagc agactctcga gcacctgctt cttcgggtgc ttgtggatct tggccaggaa
     1921 gcgagccacg ccgccgtgtt cacgtagcgc gtccacgtcg acggcgagcc ccagtttctc
     1981 ggcgatggct tgcaagcgct cgagcttgag cgggtcgggc gcggcgtgga tgcggtacac
     2041 ggcggggctc ttgcgctccc cgaggaaccg cgcgactacc tcgttggcga gcaacatcag
     2101 ctcttcgatc atcgaatacg cgcgccgaat cccggggtct tgggtgcggc gtttcacgtc
     2161 gagcggagtg cccgaatccg gatcgagcac gagcttgggc tcgggcaaat cgagatcgag
     2221 cgcgccgcgc gcgaggcgcg ctttgcgcag cttgcgcgct agctcgtcca gtaccttgag
     2281 gtccttgcgc atggcctctg cttgcgcgct gcgcgggggt tcctcggtga agccgagggc
     2341 gcgcgccacg ccgccatacg tgagcatcgc cgccgagcgc atgatgccct cgacgatctc
     2401 gaaagactcg acgtctcccg ccccgtccag atccgcgatc acacacaggc acaagcgctc
     2461 gcgttccggc aacaacgagc agagatcggc ggccagcgcg ctcggcaaca tcggaatcgc
     2521 ccgatctggc aggtaaatcg tgcagccgcg caaagaggct tcgtcgtcca gcgcggagcc
     2581 aggttctacg taagcgctga catccgcgat cgcgatatac gcgcggtaac cctccccccg
     2641 gcgctcgacc cagacggcgt cgtcatggtc gcgggcgtct tccggatcga tcgtcggcag
     2701 cggcacgtgg cgcaggtctc ggcgcccgtc cgctccgggc cgcaccagcc gcgcaaacat
     2761 gctctctgcc gcttgcacgg cgggttccgg atgcgcttct tctacgcctt cgcgcagcag
     2821 gatcttggcg acctcgacct gggggtcacc aggctccccc agcacatcga gcagttcggc
     2881 ttcggggttc tcgttggcga attcagggaa acgcgtgatg ctgaccacgg cggcctcccc
     2941 gtcacgcccc tgcttcgagt tggccgcgat cacgatcggg ccccgaatgc gagagtcgtc
     3001 gggttcgagc caggcgctct tccctcgcct tcgcagcacg ccgcctactc gcgccggccg
     3061 ccgttcgagg atctgccgaa tccgcccctc tgccccgcga ctatcacggc ctacgatttc
     3121 gatttctaca cggtcgccgt gtagtgcgcc cccgatcgaa tccggggaga tgtaggcgtc
     3181 ccctttacca ggctcggcga cgaagccgaa gccacgaggg ttcatgttta gttttcccac
     3241 ccagacttct gagtttccac gcttcgagga gctcgagcgc gggccggctt tgagccccgg
     3301 cagttttctt ttcatgtagc gccccgtata gcctggaatt cgtgatctgg cccgcctttg
     3361 agccggggac aggatgcccc aggtcggccc aaagcaggcg tcatttgctt tcttctctcg
     3421 cctgtccgtg gcggttcgaa gcccgggaaa cggtccgaag cgagcttgaa gacagcgccg
     3481 tggtgcggcg gctcggccgg cctgcgctga cactcgcgcg gtctccgcag gttccctaga
     3541 agcacgggtg ccggatacgg taagaccgat accgtggccc cgggcaaagc tcttctgctg
     3601 ctccgttccg gactactcgt agccctcgct ggctgtgcgg cgctgacgat cgagtaccgc
     3661 agcagcaacc catctttctg tagcgtcaac tccggatgtg cagcgcttcg ccgcacgcca
     3721 ctcgcgtact tgtggggggc cggcatcacg ctgccggagc tgggattaat cgcggtgtcc
     3781 ctggtttttg cgctcagttt gactcagaag cctcggattg cggccattct cacgctggta
     3841 gcgggagcgg tgggggcctt gttgatcgtg gcgcaagccc tgttcctcaa tgccttctgt
     3901 tggctttgca tgaccgtgga cgggagttcg ctgctcgcgg cattgggagc cgcagcctgg
     3961 ctgcgcgctc gcaaggacga ggcgccccca acatatgggc tgaaaacctg ggcgtgggcg
     4021 gcgttggcgg gtgtggcggt ggtcgcgccc gtagcttggc ccagcgtaaa gccggcgccc
     4081 ccggtgccac ccagtattct cgcgcagtac gtggccggca agatcaacgt gattgagttt
     4141 gccgacttcg agtgtccctg gtgccgtagc ttgcacgccc gcctcaaacc gctgctcgcc
     4201 gagtacggcg agcgcgtgca cttcgtacgc ttgaacatgc cgctcgagcg gcacccatca
     4261 gccaagggag cagcgctggc cgcgatttgc tccgagccca gcggtaaggc ccccgcgctc
     4321 gcagaattca tgttcacgac ggaggacttg agccccgaga acattcgacg tgaggttgcc
     4381 aatctcgggt tggacttggc cgagttcgat cgctgcttgg ccgctccagc gacgcagcag
     4441 cggctgacgc gagagatgga tatcctcaag caagccggtt tcgagggact gccgacgact
     4501 tacgtgggca gccggcgttt gatcggcgcg cagccggacg aggtctttcg caatgccttg
     4561 gatcgcgcgg cgcgcggcga cggcaatcgt ggcgttcccg gctggggtta cctgctgatc
     4621 gtgctcggcg tagtggcgag tattctgcgt ttcggcctag agcgccgaac gggctgagcg
     4681 atttgtcgct tggccatcgg gcgacggttt gcttgcctcc cggagctcac ggcccgctca
     4741 ccctccgacg ttactctggg ctgttactct gggctattac tgcccggagg gatcccacgt
     4801 cgtacggcct gagcggatcg cagcgcgcgc gatggctcgc atcaattcgt cgtcgatgca
     4861 gagggggagt gtcttgaaat gcggagctag actgaagctc cccacacggc agtcatcgag
     4921 tacacgaacc acgtcgtacg cgccgcgttt caagcgaacc actcggtagg tggtgccacg
     4981 aatatgaacc atcccgcgcg gtgattggga catctacgtc agttgagtca attaccccaa
     5041 ttccatgacg cactgttgag aaaacggagt tcgtcgattc gaaacgcgcg gagctaaggc
     5101 actcggtgcg agctcgcttc agaagctctc gtgataagcg gcgtcttcga tactcacagc
     5161 ctcgggggta gcctctaggg gcagactcga atccagcatg accgcaagct cgtcggtaga
     5221 gcgttttgtg ggaccgcttt cataagcgcc ggggtgtgga ccatggggaa tgccggccgg
     5281 gtgatgcgac aagctccccg caccaattcc gcgtcgcgag aggaaactcc ccgcggagta
     5341 gaacagcacc tcatccacat cgacggagct gtgcgggtat ggacacgggt tggcggactc
     5401 gtgaaagtcc agcggacgag gcacgaagct cgagacgatg agactcctac tttggaaggt
     5461 catgtgggca gttgggggca aatgtatttg tcccactcga ggttggaacg cgagaattgg
     5521 caaaaccacg gggtacacgg cgccatccca gccgaccagg tcgagggggc tgtgctcgaa
     5581 tcgaaagccg aagaagcggg accctcgctt gaccaccacg tcgcgcaggc cttcgtcatt
     5641 cggacctttg aactcgggtg tccgaaaatc acggtgcgag tagggggcgt ccattcggag
     5701 ttggccggcc ccgttcttga acgttggcgg tatgccgaag tccacgtggc tctcgagcca
     5761 gagcagcgcg tggcgatcac tggccccgag cagcacccga tagagcgtgc ctttgggcac
     5821 cagcacgtag tcgccttgat taaaagtcag atcaccctga gggctacgga gctgtcccga
     5881 cccttcgtgc acgtagaaca gctcatcgcc gtctgcgttg acgaagtaca cgcggtcgtc
     5941 ctgggtcggt tgcacgaagc cgagcacgag ctcccgactg aacagcaatg ggacacgccc
     6001 gtccaaagtc gagtgctcac ccgcgcgact cacctccccg cagcggtagt gtcgacgcag
     6061 cagcgcccgc tcgggctccg cgcgtggcac cctgaactgg cacggcgcgg gaatggcacg
     6121 cgcggcatgg gggcggtgct ggtgatagac aatcgtgtag gcaccctcga agccgccgcg
     6181 ggtgacgcac tcttcgtaca gcaaaccacc gctgtccgag tgaaacacgg tgtgaggctt
     6241 ggccggcact cggccctgct gcagacgttc gagcatcagc tcgctccttt tgtcgttgcc
     6301 tccggctccc gatcgcgttc gctaagttgt tcgcgttcga tgctctcgaa cagcgcgcgg
     6361 aaattgccgg ctccgaagcc cgggtcaccc gcgcgttgaa tcagctcgta aaagaatggt
     6421 cccgcttctt cactaccgta ggtgtgcgcc gagtccttca ggaagatctg gagtagatat
     6481 cgcccgcgcc cagccccatc caccagaatg ccgagctcct ccagccgtgc aaccggctct
     6541 gcgatgtcac ccacgcctag ttcggtcagc cgtgcccgca acgcatcgta atacgctttc
     6601 ggcgtcggca tgaactcgac gccgcgtgta cgcagccgct cgacgcaact caggatgtcc
     6661 ctgacggtga gcgcgacgtg ctgaaccccg tccccgcgat gctgctcgtg aaagacgttg
     6721 atctgagagg ctttgaagtt gggtcgatac ggctcattgc aggcgaattt gacctggctt
     6781 tggggatccc acatcaccac cgagcgcagg cccgagcccg ccttgtccac tcgttgccgc
     6841 acgtcatccg tatgaaactc cacgtgccaa aaacgctcga acccgagcac gtgctccaac
     6901 caaaggagcg ccggagacat ggtctcgaag ttgctcgtga cgtggtcgaa ggtcaggaac
     6961 ggcgccgtga cgtccgaaac cggcgcggcc agggcctcga agccgggata tagcgtgaag
     7021 tctccggtac gctcgagaaa gcgaaatgtg gtgtccccga atggagtggc gatcacgaag
     7081 ctcttgaagc cacccgttgg ggttcgggtc tcctggattt cgtggatggg cgtgccaccg
     7141 cgcgtctcca gcaatgcgaa ggcacgctcg atgttctcga cttcgaagat cagactgccg
     7201 accccgtcgg ggtgcttcgc gagatagcgg tgtgcgcgcc caccctcgcc gactgggctc
     7261 gaacacacga cgcggcaatt cccagactcg aataccagcg cgcgttgatg gcccccttcc
     7321 acgagctcgg cgccactgcg cgccgtgcag gcgaaaccga gcttgtccgt gtagaaacgc
     7381 tgactgcgct cgaggtcgtg gacgtagtag tgaatggcct cgagacgacg aataccgatc
     7441 gattcgacgc gcgccatgac actcaggata acgttgcggc gcgctcacac aaggcgacgc
     7501 ctcaaatcac cgtctggtcg atctggccaa atacgtcctt tccgtcggca gcgaaggcgc
     7561 gaatgagcac gcgatccccg gtcttcagat acggagtgac ggcttgcccc gattcgagcc
     7621 gttcacgcat gcggcgctcg accaggcacg ccactccgcg cgttgcgtca cggttcgaga
     7681 tcgtcccact acccaggatc gtacccgcac agaaggcacg cgtgcggctg atgtgggcaa
     7741 tcagctccgc gaacgaaaag tgcatctctg gccccgtctg cagctcgccg acacgctcgc
     7801 cattcagctc gcagcgcaag gtgaggaacg cgcgtgagtc acggaacgcc gaccccaatt
     7861 cgtccggggt gaccgccaag ggggagaacg ccgtggccgg cttggattgg aaaaagccaa
     7921 agcccttttc cagctcgccc ggcaccaagt ttcggtaggt cacgtcgttc gccaacatca
     7981 tgagctgaat gcggtcgcgt gcggaatgcg cattcgtgcc gagcgggacg tccgatagca
     8041 ctacggcgag ctcggcttcg aagtcgagac cgaaactcgc gtcgagcagc cgcagtggct
     8101 cggtcgggcc ggacaggacg gaagaaccgc cctgatagac caacggcgag gtctgcaaat
     8161 cggggggcag cgcagccccg cgggagcggc gcgcgagttc gacgtggttc agatatgtcg
     8221 aggcatcgat ccactcgtaa gctcggggca gaggtgcgcc acaaagctgc ggatcgaagg
     8281 gttggctcgg gacttggtcg cgctccagtc gatcctgcag cgcgtcgaat tgcggcaggc
     8341 attccgccca aatgtcgagc gcttcctgca gcgtacccac gatcgcggcc gtcgacacac
     8401 accgctcgtg gcgagcatct actaaaacca aggctccatc tctcgagcca tcacgtaagc
     8461 tagcgaatcg cattcggtgt catcctgtcc ggctcgcgct cggggcgaga ggccgcgggg
     8521 cattcttcga ttgcagcacg ccttagtgtg agctgacacg agccgacgcg cagcggccga
     8581 gctaagattg ggaacatgtc ggtctcggct cccaaagtgg cgccgttgca gcctgctgaa
     8641 gcggtgccga ggcacttgcg gcgcttcgtc gtcgagcaag attattcgca gtacaacgta
     8701 gttgaccaag cggtgtggcg cttcgtgctg aagcacacac acgctcgtct gatttcgacc
     8761 gcgcatcctg cataccattc tgggctatcc gcgacgggga tatcgctcga gcgtattccg
     8821 agtatcgaag aaatgaacga gcgcctggcg cgcttcggct ggggagcagt gtgcgtggat
     8881 gggttcttgc ctccgcgcgc cttccaatcc tttcaagccg agaggatttt gcccattgcc
     8941 ggtgagatcc gaactcgaga gcacctggtg tacacgccgg cgccggacat catccacgaa
     9001 gcctcgggac acgcgccgat cctgacggac cccgtctttt ctcgatacgt gcaacgcatg
     9061 ggctggctgg gccggcaagc cttcacgttg ccctccgagg agcgcgtgta tcaggccatc
     9121 tacaacttga gcgagattaa ggagaacccg gcgtcggacg cggcgaaggt ccagcaggcg
     9181 gaagccgaac tcgccttggc cgtgaagcat gcgggggaac cgagcgaagc ggcccgcttg
     9241 tcgcgcttgt actggtggac ggcagagtac ggcttgattg gtcgccccga ggcgtaccag
     9301 atctatggcg cagggctgct ctcgtcactt tgggagagct acagttgtca tgccgcgagc
     9361 gttagcaagc gggtcttgga cgagacgtgc atggatgtgg cttacgacat cacccgcact
     9421 cagcccgtgt tgttcgtggt gccatcgttc gaggcgctgc acggcttgct cgaccgggtg
     9481 gagcgcggca tggccgcgca agtggggggg cgctatgcgc tcgagcacgc gacgcgcagc
     9541 gcggagccag ccactttcga attcgagtct ggcgcacgcc tatacggcgt tttgaacggc
     9601 ttcgcgggag agcggacaaa acggctgcaa tttggcggat ccgtacgggc ggtctggcgc
     9661 atgggcgaac cgagcatcgc gctcggggcc gacctctgca tcgtcgcggg ccgcactctg
     9721 gacgggcaag gcctggcgga atggatggtc caggggtctc atggtgcttc cgggcgcgtg
     9781 caggttcagt ttgcagaagg gactagcttg gtgggccgcc tcgccgcgca agccgtgacc
     9841 ggcccagacg gagccgcgcg ctgtctgcag ctcgaggacg tagaactccg cagcccggac
     9901 tcccctcctc ggtttttccg cgaatacacg ctgctcgctc taggcgaatt cgtaactgcg
     9961 cgcgcagggg ccgacagcga tctcgaacgc gagttgtctt tgtacgagcg tacgctctcg
    10021 accgagcaaa agacgccgtc gccctggcga gtgccgcgcg tccgcgtgtt tgccgagcct
    10081 gagctgcgcc tgcaggcgct ctactcgcga tgggagcgag tgcgggagtc ggccttcgcg
    10141 gagcagagca tggagatacg agcactctat gccgcactgg cagagtatcc gaacgagtgg
    10201 ctactgcgac tccagctact gcagggcgcg acgcgcatgc ccgagctcgc ggacttgcag
    10261 cgaaccctgc gcggggagct cgagcgcctc gaactgctgt tcgagggtcg ggagccaatc
    10321 gaaacggggc tgcgcgaagt cgggcacgca gcctgaactc cttcgagtga ccgactcgtg
    10381 tggcgccgcg ggatgtcttc agcgcgtggc cctgagcggt tattggaggg gccggcgcgg
    10441 tgaaggaggg ggaacgcggg ggttagggct gcgctggtgg gggatgggac gggcggcgcg
    10501 cccgtggtga ggtggccgac aaagggcttg ccgcggcgat caggtgtcgt cgagatcttc
    10561 ttcgagacgg cgcgggatct cgtgaccccg gagcgagcga cgtagttcgt cgcagagctc
    10621 ggtctccgga cgcgcgacct cgggtcggga gtgctcggcc cgttcgacgg tgttgtgctc
    10681 gagtgcggtg acgatggcaa cgcgcacgcc ttccggcagc aggctctcca ggtattcgag
    10741 cgcagtgcca cgcagcttct ggtctgggct gcgcaggcct tggcgcacga gctcggcgtt
    10801 ctgttggcct gtggcgagcc cgatcagggt gaacacgtgg tcgctggtgg gcacgtcgag
    10861 cggggccttg gcgctttcga gcacagccaa gtgcagcagt ttttgcggct cggggtg
//
LOCUS       DASTKU010000282         8314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_723344, whole genome shotgun sequence.
ACCESSION   DASTKU010000282 DASTKU010000000
VERSION     DASTKU010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723344"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="VFQ61_10150"
     CDS             <1..1377
                     /locus_tag="VFQ61_10150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9954857.1"
                     /translation="PEVSRVHARISSTPSGQFEIHDLDSKNGVFVNGARVTHCQLDFG
                     DKVGIGQKALFEFTGFNAVEDYIVQRQRYEAIGRLGVGIAHDLNNVLAALDAGAAFLR
                     DLPTERRLGDKDVRECIADLSLAAVRASELTRGILSFARGSSAQRGAVDLSALVHEVV
                     RMLRHTLDHSVRVEANTDAQSWVYGSHSELHQILLNLCLNARDAMPQGGTLRLFAARL
                     KQAPQSAGELAGKVVAALSVADSGVGMDDELQRRIFEPFFTTKERGAGYGVGLATVRE
                     IVSRHGGVITVESTPGQGSRFTVYLPSHELDDARRTRHSVPSLMPSRPLDLVKILLVD
                     DEQIVRRSVARLLRQAGFQVVEAESGADALAMYQRSPFDLVLLDVDMPDMDGEQTQMR
                     LVALDPYVRIVFASGHHEPLREAAVRARGALGFLQKPFALESLVEIVNSVLLVEVPDE
                     LDEPTRPR"
     gene            complement(1418..2095)
                     /locus_tag="VFQ61_10155"
     CDS             complement(1418..2095)
                     /locus_tag="VFQ61_10155"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET9954858.1"
                     /translation="MTESSPSDPALEALFDAGFPRKPVDELRANVQREVEACLSSSDP
                     SSALWHAHELAERALHQIERERRHLKTVPRVACRAGCSSCCFLRVEVTAAEAELLWPL
                     VRDLELLDKVRATSERVSGLDRLARLRERVPCALLSEAGSCLVHERRPFACRAANSLD
                     EKACEAHVASANVLGEVPIDPVPFAVQRATALGFGSGLRSHGLPAVVGELHAMLCACQ
                     GPGRETR"
     gene            complement(2092..3672)
                     /locus_tag="VFQ61_10160"
     CDS             complement(2092..3672)
                     /locus_tag="VFQ61_10160"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET9954859.1"
                     /translation="MTPFEHAVRRRRTFAIISHPDAGKTTLTEKLLLFGGAIQAAGAV
                     KARGEQRRARSDWMKVERERGISVTASAMTFEYDNITFNLLDTPGHQDFSEDTYRTLT
                     AVDSAIMVIDAAKGIEEQTRKLFEVCRLRNVPIITFVNKLDREGRDPFDLMDEVEQTL
                     ALDVTPASWPIGMGKGFLGCYDLFHDRLVLMERARERLEDAGETCEGLDDPKLDQLLP
                     EDRVRALRDEVTMARGLCPDFDLQSYREGHLTPVFFGSAISSFGVRELLLGLAELAPS
                     PRPFNTKERTVEPAEERVTGFVFKVQANMDPKHRDRVAFVRLCSGHFRRGMKLFHSRS
                     GGMLAVHNPVLFLAQDRELADEAVAGDIIGIPNHGKLGIGDSLTEGEELHFTGIPRFA
                     PELLQRVRATDPMRTKHLARALEQLAEEGVARIFKPRMGGYYIVGVVGALQFDVLADR
                     IKSEYEIPVLFEPAGLFTARWVSAQDPKQIETFMSRNEAYVADDHNGDPVFLARNAWH
                     LETTQKDFPELSFQKTRE"
     gene            3832..6375
                     /locus_tag="VFQ61_10165"
     CDS             3832..6375
                     /locus_tag="VFQ61_10165"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HET9954860.1"
                     /translation="MSVRRSTLVLASTLGAVAVVWFAWPTRPRDAQRPNPKLPYASRG
                     VTISSVSQPGLSKAESVAAETGDWLVQNGRLSFSVGADAAGLERRAHHGTLLDLALGD
                     FNADELVEFRPVVRIANSPLPLNMVGVSLLTEGTFPFLRVDEKSRDGQLEVMTDYLAP
                     PGQAFIEVVTRLHNAGEVRVSAVELGERVRWPGGALFSPRVGFPRAPVRAELNWLARE
                     GAQLTYALVFPRGPVQGSFLFDRVGPVGQETLAQVGDAEPGQTIEHRRLLLVVPNVPP
                     APGAKSTSPGPGGPSGQGLGGAAALAWKAMQRELGYVSGRIEAQVAWAVVEADYPDGK
                     PALIVQARTDGSFELPLVPGEYRITLRTPGGKDSVDVRVAAGQTVSTHLIAPVPGRLS
                     YSVTDEAGAALPARILLRGVAPTPDPELSASDPSAVSRNVIYSRGGTGHVELPPGHYR
                     AVVSHGSEYEIASQDIEVDGEDGASLRAVLQHSVETGGWLACDFHLHQAPSHDSSVSL
                     PDRVLSLLAEGIEFAVATDHNHVTDFMPAVLEQSAAPMLGVASGVEVTTSSWGHFNAY
                     PYPIKGNPPPFSGVSPIEIFASIRARAPGAVIQVNHPRMPGVGYFNRIELDPQTGAAA
                     TEGASWSFDAVEVVNGYDLEANELIQQNLQEYFTLLNFGRRITATGNSDSHRLLINWA
                     GYPRTYVRVNDEHPGSVNPADVARAIVEGHASVSNGIFLAVVANGTLGPGDMVTGRRV
                     DLQVQARAPRFVDVEEIEVWVNGTVRDHTRGLSRASSVRMALRSQLSVDRDAWIVVVA
                     RGKDPMDSVFLNRRVLPFAFTNPIYVDADEDGTFRAPLAPELPTAPLAH"
     gene            complement(6397..7353)
                     /locus_tag="VFQ61_10170"
     CDS             complement(6397..7353)
                     /locus_tag="VFQ61_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954861.1"
                     /translation="MRILSALMLGYYFFVGRVGGDAGVVPGGTPVVPTGMTAAARAEL
                     DRAVGVAAAELAPREVSPPLRKVTLGGRVISIPEGCRSVEKPYNLVIHFHGAPTSMEP
                     AFEKSGIGGVLAILNLGIGSGKYEDAFQAPSAYPELLRRVSEVVREMCPGADASGRFK
                     HVALSAWSAGYGSVWRILDRQNHADQVDAVLLADGLHAGFEPDGERTRTVNSAQMAAF
                     ALYADRAASGEKLFAITHSSILTPYASTTETSSYLLEQENVARVALNAQGPRANMMLI
                     SRGDRGNFHVQGYAGNDKPDHCDHLHAIGDTLFPYLRERWSR"
     gene            7710..>8314
                     /locus_tag="VFQ61_10175"
     CDS             7710..>8314
                     /locus_tag="VFQ61_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9954862.1"
                     /translation="MQVYGMRIDGRVVSSDVTREVRSPYDGSLVGQVALGNEGHLAGA
                     VASASRAFGDFSRSPTHVRATLLERIAQGIGARSEEIAHLITRESGKPIRYSRAEVLR
                     GQSTFTLAAAEARAFGGEVLPADQLPGQEGRWCLYKRVPRGPVAAISPFNFPLNLVAH
                     KVAPALAVGATVVLKPAAQAPLTAHLLSEIVDQAGAPAGVF"
BASE COUNT         1430 a         2654 c         2659 g         1571 t
ORIGIN      
        1 ccagaggtct ctcgcgtaca cgctcgcatc tcgagcaccc cttcgggcca attcgagatt
       61 catgatctcg acagcaagaa tggagtgttc gtcaatggtg cgcgggtcac gcattgtcag
      121 ctcgacttcg gtgacaaagt cggcatcggt cagaaagctc tcttcgagtt caccggcttc
      181 aacgcggtag aggattacat cgtacagcgg cagcgctacg aggccatcgg gcggcttggc
      241 gtaggcatcg ctcacgatct gaacaatgtg ctggccgcgc tcgatgccgg cgccgccttc
      301 ttgcgggacc tgcctacgga acgtcgactg ggagacaagg acgttcgcga atgcatcgcc
      361 gatctctcgc tcgctgcggt gcgagcaagc gagttgacac gaggtattct ttccttcgcc
      421 cgaggcagct ccgcgcagcg gggggcggtc gacctatcgg cactggtgca tgaggttgtc
      481 cgcatgttgc gtcacacgct ggatcacagc gttcgcgtgg aggccaacac cgatgctcag
      541 tcttgggtct atggtagcca ctccgagctg catcagattc tgctgaatct ctgcctcaac
      601 gcgcgggacg cgatgccgca aggcggaacg ctgcggctct ttgcggcgcg cttgaagcaa
      661 gcgccgcaga gcgcaggcga gctcgcgggc aaggtcgtgg ccgcgctctc ggtcgcggac
      721 agcggcgtcg gtatggatga cgagcttcag cgccgcatct tcgagccatt cttcacgacc
      781 aaggagcgcg gcgccggtta cggcgtcggt ttggcgactg tgcgcgaaat cgtttctcgg
      841 catggagggg tgatcacggt cgagagcacg cccggccagg gcagccgttt caccgtctat
      901 ttgccgagcc acgagctgga tgacgcgcgc cgcacccgcc actcggtgcc gtcgctgatg
      961 ccgtctcggc cgctcgatct ggtaaagatt ctgctcgtcg acgacgagca gatcgtgcgt
     1021 cgtagcgtcg caaggctact tcgccaagct ggttttcagg tcgtggaagc ggagagcggc
     1081 gccgatgcgc tggcgatgta ccagcgttcg ccgttcgacc tcgtcttgct cgacgtggac
     1141 atgcccgaca tggacggaga gcagactcag atgcggctgg tcgcgctcga tccgtacgta
     1201 cgcatcgtgt tcgcgagcgg gcaccacgaa ccgctgcgcg aggccgccgt gcgcgcgcgc
     1261 ggcgcgctgg gcttcctgca gaagccgttc gcattggaaa gcttggtgga gatcgtcaac
     1321 agcgtgctgc tggtcgaagt ccccgacgaa ctggacgagc ccacgcgccc gcgctgacgc
     1381 tcgtcgaggc cgtcgcgttt cgcggccttc aactcagtca gcgcgtttcg cggccgggtc
     1441 cctggcaagc gcagagcatc gcgtgcaatt ctcccaccac ggccggtaag ccgtggctcc
     1501 gcagtccgct gccgaagccg agggccgtgg cgcgttgaac cgcgaagggg acgggatcga
     1561 tcggcacctc gccgagcacg ttggcgctcg cgacgtgcgc ttcacaggct ttctcatcga
     1621 gtgagttcgc cgcacgacac gcgaacgggc ggcgttcgtg caccaaacac gagccggctt
     1681 cggagagcag cgcacagggc acgcgctctc gtagccgggc gagccgatcc aatccgctca
     1741 ctcgctcact cgtggctcgg accttgtcta gtagctcgag gtcgcgcacg agcggccaca
     1801 gcagctcggc ctccgcagcc gtgacctcca cgcgcaggaa acagcaactg ctgcagcccg
     1861 ccctacaagc tactcgaggc accgtcttca agtgccggcg ctcgcgttcg atctggtgta
     1921 gcgcccgctc ggcgagctcg tgcgcgtgcc acagtgccga gctcggatcg ctgctgctca
     1981 agcaagcctc tacctcgcgc tgcacgttcg cgcgcagctc atccaccggt ttgcgaggaa
     2041 agccggcgtc gaacagggcc tccaaggctg ggtcgctcgg gctggattcg gtcactcgcg
     2101 agtcttctgg aaggacagct ccgggaagtc tttctgagtc gtctccaagt gccaggcatt
     2161 tctcgccaaa aataccgggt cgccgttgtg gtcgtccgcc acgtacgcct cgttgcggct
     2221 catgaatgtc tcgatttgct tcgggtcctg ggcgctgacc cagcgcgccg tgaacaaacc
     2281 cgcgggctcg aacaggactg ggatttcgta ctcgctcttg atgcggtccg cgagcacatc
     2341 gaactgcagc gcacccacca cacccacgat gtagtatccg cccatgcgcg gcttgaaaat
     2401 gcgcgccaca ccttcctccg cgagctgctc gagcgcgcgc gccagatgct tggtgcgcat
     2461 cgggtcggtc gcgcgcacgc gctgcagtag ttccggcgcg aaccgcggga ttcccgtgaa
     2521 gtggagctcc tcaccttcgg tcaatgaatc gccgattccg agcttgccat gattgggaat
     2581 tccgatgatg tcccctgcca ctgcttcgtc cgcgagctcg cgatcttggg ccaagaacaa
     2641 cacgggattg tgcactgcca acatgccgcc cgagcgcgag tgaaacagct tcatgccgcg
     2701 tcgaaaatga ccggagcaga gccgcacgaa cgccacgcgg tcgcggtgct tggggtccat
     2761 gttcgcctga accttgaaga cgaaccccgt cacccgttcc tccgccggct ccaccgtgcg
     2821 ctccttggtg ttgaagggcc gcggtgatgg cgcgagctcg gccagaccga ggagcagttc
     2881 gcgtacaccg aagctgctga tggcgctgcc gaagaagacc ggggtcaagt ggccttctcg
     2941 gtagctctgc agatcgaaat ccggacacaa accacgcgcc atggtgacct cgtcccgtaa
     3001 ggcgcggacc cgatcttccg gcaagagctg gtcgagtttc ggatcgtcga gcccttcaca
     3061 ggtctccccg gcgtcctcca agcgttcgcg tgcgcgttcc atcagcacca accggtcgtg
     3121 gaacaggtcg taacaaccca aaaatccttt ccccattcca atcggccagc ttgcgggggt
     3181 cacgtcgagc gccaacgttt gttcgacctc atccatcaga tcaaacggat cgcgaccctc
     3241 gcgatccagc ttgttcacga acgtgatgat gggcacgttc ctcaatcgac acacctcgaa
     3301 gagcttgcgc gtctgctcct cgatgccctt ggccgcatcg atgaccatga tcgccgagtc
     3361 caccgccgtc agcgtgcgat aagtatcttc gctgaagtct tggtggcccg gcgtgtcgag
     3421 taggttgaat gtgatgttgt cgtactcgaa cgtcatcgcg gacgcggtaa ccgagatgcc
     3481 gcgctcgcgt tccactttca tccagtccga gcgcgcccgg cgctgctcac cgcgagcctt
     3541 caccgcaccc gccgcttgaa ttgccccacc gaacaacagc aacttctcgg ttagggtggt
     3601 ttttcccgcg tcaggatgag agatgatggc gaaggtccgg cggcggcgaa cggcgtgctc
     3661 gaagggagtc atgaggcccg cctctcttag cttggatttt cctggccgca tgaggcatgc
     3721 cgcgaagacc tcaaatcttg gtgcggccca cgggtcggca tgcctgctcg cggacagatc
     3781 tctaggcaga gtgctctgcc ccgcgggcgc tctcggtgta gcctccgcgc cgtgtcggta
     3841 cgacgcagca cgcttgttct cgcctcgacc ctgggtgcgg tggccgtcgt ttggtttgcc
     3901 tggcccactc gcccccgcga cgcccagcgc cccaacccca agctgcccta cgcatcacgc
     3961 ggggtaacga tttcatccgt gtcacagcca gggctttcca aagccgagag cgttgcggcc
     4021 gagacggggg actggctggt acagaacggg cggctctcct tcagcgtcgg tgcagatgcg
     4081 gcgggtctgg agcggcgcgc ccatcacggc acgctgctcg acttggccct gggagatttc
     4141 aacgccgatg agttggtgga gttccgcccg gtcgtgcgca tcgcgaatag cccgctgcct
     4201 ttgaacatgg tgggggtgag cctgctcacg gaaggcacgt tcccgtttct gcgcgtcgac
     4261 gagaagagcc gcgatggcca actcgaggtc atgaccgact atctggctcc gcccggacaa
     4321 gccttcatcg aagtggtcac tcggcttcac aacgccggtg aagttcgggt gagcgcggtc
     4381 gagctaggag aacgagtgcg ctggcctggc ggcgcgcttt tctctccccg cgttggtttt
     4441 ccacgcgcgc cggtacgggc cgagctcaat tggttggcgc gcgaaggggc gcaactaacc
     4501 tatgccttgg tctttccacg cggcccggta caaggatcgt ttctcttcga ccgggtgggt
     4561 cccgtagggc aggagacgtt ggctcaagtg ggtgacgcgg agccggggca aaccatcgag
     4621 catcggcgtc tgttgctcgt cgttccgaat gtcccgcccg cgccgggcgc gaaatcgacc
     4681 tcccctggac cgggtgggcc ttcagggcag gggcttggcg gagccgcggc gttggcgtgg
     4741 aaagcaatgc agcgagagct tggctacgtg agtggtcgca tcgaggccca agtagcgtgg
     4801 gccgtcgtcg aggccgacta ccccgatggc aagcccgcgc tgatcgtgca ggctcgcacg
     4861 gacggctcgt tcgagttgcc gctggtcccc ggcgaatacc ggatcactct gcgcactccg
     4921 ggtggaaaag actcagtgga cgtgcgggta gcggccggac agaccgtctc gacgcatctc
     4981 atcgctcccg taccaggacg actcagctat tcggtgaccg acgaggcggg ggccgccttg
     5041 cccgcgagga tcttgctccg cggggtcgct cccactccgg atcccgagct gtccgcgagt
     5101 gatccgagtg cagttagccg caacgtcatc tattcccggg gcggaacggg tcacgtcgaa
     5161 ctcccccccg ggcactatcg agccgtggtc agtcacggct ccgagtatga aattgccagc
     5221 caggacatcg aggtggatgg cgaagacggt gccagtttgc gtgcggtctt gcagcattcg
     5281 gtggagaccg gcggttggct cgcttgcgat ttccatctgc atcaggcgcc ttctcacgac
     5341 tcctcggtga gtttgccgga ccgcgtgctc tccctgctcg cggagggaat cgagttcgcg
     5401 gtcgcaaccg accacaatca cgtgaccgac ttcatgcccg cggtgctcga acaaagcgca
     5461 gctcccatgc tcggagtggc gtcgggcgtg gaggtaacga cctcgagctg ggggcacttc
     5521 aatgcgtacc cgtacccaat caagggcaat cccccgccgt tcagcggagt cagtccaatc
     5581 gaaatcttcg ccagcatacg tgctcgtgct cccggcgcgg tcattcaggt caaccatcca
     5641 cgtatgcccg gagtcggcta cttcaaccgg attgagctcg atccgcagac gggagctgcg
     5701 gcgacggaag gtgcgtcgtg gtcgttcgac gcggtggagg tggtgaacgg ctatgacttg
     5761 gaggcgaacg agctgattca gcagaattta caggagtact tcacgctgct gaacttcggg
     5821 cggcgcatca ccgcaaccgg caactcggat tcccaccgct tgctgatcaa ttgggcggga
     5881 tacccgcgga cctacgtccg cgtgaatgac gagcaccccg gctcggtcaa tcctgcggac
     5941 gtggcgcgcg ccatcgtcga gggccacgcc agcgtctcga acggcatttt cctagctgtt
     6001 gtcgcgaatg gcacgctggg tccgggcgac atggtgacgg ggcgtagagt ggatttacag
     6061 gttcaggctc gcgccccgcg cttcgtagac gtggaagaaa tcgaggtctg ggtcaacggt
     6121 acggttcgcg accatactcg gggactatcg cgggcctcgt cggtgcgcat ggcattgcgg
     6181 agccagcttt ccgtcgatcg ggacgcctgg atcgtggtcg ttgcccgcgg caaggacccg
     6241 atggactcgg tctttttgaa tcgccgcgtg ttgccgttcg cattcacgaa cccaatctac
     6301 gtcgacgccg acgaggacgg cacgttccgc gcaccgctcg cgccagagct accgacagcg
     6361 ccgctcgcac actgacgcgc ggaaacgggc cgcagctcag cgtgaccagc gttcgcgcaa
     6421 gtatgggaac aacgtgtcac caatcgcgtg caggtgatca cagtgatcgg gcttgtcgtt
     6481 gccggcgtag ccctgcacgt ggaagttgcc gcgatcgccg cgcgaaatga gcatcatgtt
     6541 ggcgcgcggg ccctgagcgt tgagcgcgac gcgcgccacg ttctcctgtt cgagcaggta
     6601 actcgacgtc tccgtggtgc ttgcgtaggg cgtcaggatt gacgagtgcg tgatggcgaa
     6661 cagcttctcg cccgaggcag cgcgatcggc atatagcgca aacgcagcca tctgagccga
     6721 gttgacggtg cgggtgcgct ccccgtcggg ctcgaagccc gcgtgcaaac cgtccgcgag
     6781 cagaaccgcg tccacctgat cggcgtggtt ttggcgatcc aagatgcgcc atacgctgcc
     6841 gtagcccgcg ctccaggcgc tcaacgccac gtgtttgaat cgaccgctgg cgtctgcgcc
     6901 ggggcacatc tcgcgcacga cctcggagac tcgacgcagc agttccggat aagccgacgg
     6961 cgcttggaac gcatcttcgt attttccaga cccgatgccc agattcagaa tcgcgagcac
     7021 cccgccgatg ccgctctttt cgaaggccgg ctccatcgac gtgggagcgc cgtgaaaatg
     7081 gatcacgagg ttgtatggct tttctaccga gcgacacccc tcggggatgg agattacgcg
     7141 cccgcccagc gtcaccttgc gcagcggagg gctcacctcg cgaggcgcca actcggcggc
     7201 ggccacgcct accgcgcgat cgagctctgc gcgcgcagcg gcagtcatgc ccgtaggcac
     7261 taccggtgta cctccgggta cgacccccgc atcacctccg acgcgcccga cgaagaagta
     7321 atagccgagc atcaacgccg aaagaattct catcggagtc gtatttataa aagaggccgg
     7381 aacggctcgt ctaccgcaaa ctcaacacga tttgcgatcg gtcacacctt gccagtgggg
     7441 ctgagttttt cccccgcctt tcctctggtc tggattgttc tggtcccatt gagatcggtc
     7501 gaggggcagc cggagcgcct ccagacggcg ctcttcctcc gcgaatttcc gcgaatgcgg
     7561 cgcagctcgt ggcgaatggc tactgtccat caggggcatt tcgagcgtcg agccgtggtt
     7621 tcaggtcact cgagccacga gcttgaaagc gcgtacctag tcagttgggt ttctggcgcg
     7681 ggcacatttt cggcggtatt cgtaaaatca tgcaggtata cgggatgagg atcgacggcc
     7741 gggtcgtgtc gagcgacgtg acgcgcgagg tgcggagtcc gtacgacggc tcgctcgtcg
     7801 gacaggtcgc actcggaaac gagggccatt tggcaggcgc cgtcgccagc gcatcccgag
     7861 ctttcgggga cttttctcgg agcccgaccc atgtgcgtgc gacgttgctg gagcgaatcg
     7921 cgcaggggat cggcgctcga tcggaggaaa tcgcgcacct catcacccgc gagagtggta
     7981 agccaattcg ctactcacgc gcggaggtgc tgcgtggaca gagcaccttt accttggccg
     8041 cggcagaggc gcgcgctttt ggcggggagg tcttgccggc ggatcagctg cccgggcaag
     8101 aagggcgttg gtgcctttac aagcgcgtcc cgagggggcc ggtggcggcc atttctccct
     8161 tcaatttccc gttgaatctg gttgcccaca aggttgctcc ggcgcttgcc gtcggtgcca
     8221 ccgtggtcct gaaacccgct gcgcaggccc cgctaaccgc gcaccttctc tccgaaatcg
     8281 tcgaccaagc gggcgcgccc gcgggcgtct tcaa
//
LOCUS       DASTKU010000283         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_723496, whole genome shotgun sequence.
ACCESSION   DASTKU010000283 DASTKU010000000
VERSION     DASTKU010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723496"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VFQ61_10180"
     CDS             complement(<1..1076)
                     /locus_tag="VFQ61_10180"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HET9954863.1"
                     /translation="MRSPSYERAMRWGISRPADSALLFTCLYFALACSQQSGGGQQSG
                     GAESKGGGATASGGTTSRGGGSSTQGGGVASGGAPGAGGAIGQGGSGQSGGAQSSGGS
                     GGMASDGGSSSPTRGGAANGGGSSMQSGGAASGGGNTAKGGGAGTSGGSDGGSGGTGG
                     SAASDCTPKGRAHNPLVTQIFTADPNAIVYGDRVYVYTSHDVDGQTGFDMVDYHAFSS
                     DDLVNWQDHGVIIQASELPWATNLYAPGACAKNGKYYLYIPNSGSGIGVAVADKPGGP
                     FVDPLKKALITKSFPNANVPWLFDPACFVDDDGQAYLYFGGGDDGGQNARVVRLNDDM
                     ISIKDSSATKIPTTAFFEASFMHKR"
     gene            1269..2354
                     /locus_tag="VFQ61_10185"
     CDS             1269..2354
                     /locus_tag="VFQ61_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954864.1"
                     /translation="MTPTIGANNATGFVSGELGWTLSTPLRRTLARSPRETIALPTPE
                     AVISRIGLETKRRLARCEAHGLHYDSDAAGCVLCRKSLAPAPSRAPVAATPLEQPTLG
                     TGTWLRLVLLVPLVGLVAVLTAANEGRPLSAMHAASAFGSILLAALLCGIVGRWRNNR
                     AFSKVALVFALLSLGLQLADWTRARTAAARVKGAANTSTSGGAAALQPVRAPLGNIEL
                     RLPASWTLLDPGRMGPYSAGAASPRGDAVFAQTEPLGRYEARVDLAVYGELVRSRKLG
                     PDYALRYGLPEARTISGVPAERCSMEGTFKGAPMNGYLYVVKGATGFVNLVAYATPER
                     WQTMEQELVTLVEHSRLDEGATETDAP"
     gene            2427..>2757
                     /locus_tag="VFQ61_10190"
     CDS             2427..>2757
                     /locus_tag="VFQ61_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9954865.1"
                     /translation="MVGIDLGTTHSSVALLRDAEVHVIQNTHDEVLTPSAVALDPHSG
                     KMLVGRAAKDLLSLHPERGASSFKRWIGTSRTQRIGDREFNATELSAFVLDALRADAE
                     RALGKRVE"
BASE COUNT          464 a          910 c          847 g          536 t
ORIGIN      
        1 cgcttgtgca tgaacgaggc ttcgaagaag gcggtcgtcg ggatcttggt cgcgctcgag
       61 tccttgatgc taatcatgtc gtcgttcaga cgaacgacgc gcgcgttctg gccaccgtcg
      121 tctccaccgc cgaagtagag gtacgcttgg ccatcgtcgt cgacgaagca cgcggggtcg
      181 aacaaccaag gcacgttggc gttgggaaag ctcttcgtga tcaaagcctt cttgagcgga
      241 tcgacgaaag gaccgcccgg cttgtcggct accgcaaccc caatccccga accgctattg
      301 gggatgtaga ggtagtactt gccgttcttc gcgcacgctc ccggcgcgta cagattggtg
      361 gcccaaggca actcgctcgc ctggatgatc acgccgtggt cttgccagtt caccaagtcg
      421 tctgacgaga acgcatggta gtcgaccatg tcgaagccgg tctggccgtc gacatcgtgt
      481 gacgtgtaga cgtacaccct gtcgccgtag acgatcgcgt tcgggtccgc ggtgaagatc
      541 tgcgtgacca acggattgtg ggcacgtcct ttgggcgtgc agtcggacgc ggcgcttcca
      601 ccggtccctc cagagccgcc gtcgctcccg ccgctcgttc cagccccgcc gcccttcgcc
      661 gtgttgccgc ccccgctcgc ggccccaccg ctctgcatgg aacttccgcc accgtttgcc
      721 gcaccaccgc gggtcggtga agagcttccg ccgtcactcg ccattccgcc gcttcccccg
      781 ctgctctggg cgccgccact ttgcccactg ccaccctgtc caatcgctcc gcctgcgccg
      841 ggtgcgcccc cgctcgcgac gccgccgccc tgcgtagaac taccgccgcc gcgactcgtg
      901 gtgccaccgc tcgccgtagc accaccgcct ttgctttctg caccgccgct ttgctgacct
      961 cctccactct gttgtgagca ggccaacgcg aagtacaggc acgtgaacag caaagccgaa
     1021 tcggcgggac gcgaaattcc ccaacgcatg gctcgctcat aggatggact gcgcatcctc
     1081 gcaagcaatt gcgatgtgta ttcgactgtt ggagttcagc gatgcattcg tgatgcttcg
     1141 tttacgaaat atgagcgatg tcgctgacgg aaacgccaac actgcgccac tgcgcgccga
     1201 gcgcgagcgc ggagtgaggg taagactctt gatcatctgc tcccggatcc gagagatgtt
     1261 gggttcggat gaccccgacc atcggagcaa acaacgcaac cggttttgtt tccggcgagc
     1321 ttggatggac gctttcaaca ccgctgcgac gtacgctcgc ccgtagccct cgtgagacga
     1381 ttgcgttgcc cacccccgag gcagtaatct cgcggattgg cttggaaacg aaacgacgat
     1441 tggcgcgctg cgaggctcac ggcctgcatt acgattccga cgctgctggc tgcgtcctct
     1501 gccgcaagtc tttggcgccg gctccctcgc gggcccccgt cgccgcgaca ccactagagc
     1561 aacctaccct cggaacgggc acctggttgc gccttgtctt gctcgtgccc ctggtgggcc
     1621 tcgtcgccgt gctcacggcc gcgaacgagg gtcggccact gagtgcgatg catgccgctt
     1681 ccgcgttcgg aagcatcctg ctggcagcgc tcctctgcgg catcgttggg cgttggcgta
     1741 acaaccgagc tttcagcaag gtggcgctgg tgttcgctct gctttcgcta gggctacagc
     1801 tcgcagattg gacccgggcg cgtacggccg ccgcgcgcgt caagggcgct gcgaacacga
     1861 gcacgtcggg cggtgctgcc gcgctgcagc ccgttcgcgc gccactcggc aacatcgaac
     1921 tgcgcctgcc cgcctcgtgg acactgctag accccggcag gatgggtccg tacagcgctg
     1981 gagctgcctc gccccggggc gacgcagttt ttgcacagac ggagcctctc ggccggtatg
     2041 aagccagggt cgatttggcg gtttacggtg aattggtccg ctcccgcaag ctcgggcctg
     2101 actacgctct tcgttacgga ttgccggaag cgcgcacgat ctcgggcgtg ccggctgagc
     2161 gttgttcgat ggaaggcacg ttcaagggcg cgccaatgaa cgggtatctc tacgtcgtca
     2221 aaggggctac gggcttcgtc aacctggtcg cttacgcgac gccagagcgc tggcagacga
     2281 tggaacaaga gctcgtcacg ttggtcgaac attcacggct cgatgaaggt gcaaccgaaa
     2341 cggatgcccc ttgagccgga ttcagattca ggagcgttga tctcgggctt cggtacttct
     2401 cagcattcgg ggatgccttg gagatagtgg tcggaatcga tctaggaacg acgcacagct
     2461 cggtggcgct gttgcgcgac gccgaggtgc acgtgattca gaacacgcac gatgaggtcc
     2521 tcacgccctc ggcggtcgcg ctcgatcccc attccggcaa aatgctcgtc ggccgcgccg
     2581 caaaggatct cctctcgctc catcccgagc ggggtgcctc gagcttcaag cgctggatcg
     2641 gcacgtcgcg aactcagcgt atcggcgacc gcgagttcaa cgcgaccgag ctctccgcat
     2701 tcgtgctgga tgcactgcgt gcggatgccg aacgagccct cggaaagcgc gtcgaac
//
LOCUS       DASTKU010000284        27689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_946990, whole genome shotgun sequence.
ACCESSION   DASTKU010000284 DASTKU010000000
VERSION     DASTKU010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 27689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27689
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946990"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="VFQ61_10195"
     CDS             complement(<1..1724)
                     /locus_tag="VFQ61_10195"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HET9954866.1"
                     /translation="MFTKTEGFRHDSIADAQEFFSELPAEQRISATLTEDSAVFDDAT
                     LSAYEAVVFANTTGDVLAPAQQAALQRYLRGGHGFVGIHAAADTEAGWPWYVGLVGAT
                     FVSHPESPLEVDVHVEEGTHPAVQPFTPQFRFEDEWYNFDRNPREVATVLLSIDERGF
                     TVPNTPPGPSMGADHPVAWYQQYEGARSFYTNLGHRPQTWHDTRFQQHILEGIRWAAR
                     GGGFGRAVLTRNAKSPIALAVRPDGDVYFIERTGELKLWRARTGAVEVAFRVEVDTAQ
                     ENGLLGLALDPSFAKNGFVYLYYSAPLSGEDVAGGPPGRNELARFKSRADGHLDEASR
                     QILLEVPSERRCCHEGGSLAFAADGTLWLATGDNTNPFDSAGAAPLDERTGRETFNAQ
                     RTSANPFDLRGKILRIRPDGGVPEGNLFGADERDGRKEIYALGVRNPYRLAADPKLDR
                     VYFSDIGPDAVTDSDRGPRGYDEINWARPGNYGWPYCIAEQLPYANYDFANQSIGAPY
                     DCEGTIPAWMAYDYGTPRYPALGPGLLTDGSVVGRAAIAGAVYRAPAGARFAFPSRFD
                     GALLISDWT"
     gene            2215..3675
                     /locus_tag="VFQ61_10200"
     CDS             2215..3675
                     /locus_tag="VFQ61_10200"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9954867.1"
                     /translation="MAAQAGASGQPSLPLSEDGRVPPDRLTLGTKLAYAVGGTSDIFG
                     HWLYNTLVDPVYNVFLGLAPTQISITRALTLLVDACSGLFFGWLSDNTRTRWGRRRPY
                     VLVGSILAGAGLPCLFLARPSWSSEATFWYMLVSACLYAPLIACFNTPFQSLGAELTP
                     DYNERTSVMSYKGVIQKIAGALITGALWFATRPMFNNPVTGKPDVARGAMWAAALAGG
                     WMAFSGIVNFAFVPERYYSKAKQQARASFIGMFRDALSCRPYLILLGAALMYAVPTGL
                     VGTLGFYALTYHVFAGDMTRASTLLFWGGIAYTVLGVMGIAAANHMARRFGKHRTLIG
                     ALLIGLVAFASSFWLYTPTYPYLSMVTGGLNGFSATGLWVVLPAMTADVVDYDELHSG
                     KRREGAYTATFSWVMKVGMMLSMLIGGPLLELTGFNAKLGGAQAPSTILWIRILFAGI
                     PVTALVLALLLIRLYPLGQARVTEMRRELERRRGAV"
     gene            complement(3596..4498)
                     /locus_tag="VFQ61_10205"
     CDS             complement(3596..4498)
                     /locus_tag="VFQ61_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954868.1"
                     /translation="MISLLETHLRFGRGAQSTGPSTWRAIGLLATISSAALGFASGDF
                     LAPGSSAAKTLLRPATYAFAISLPVYASLIAYGSSALRADQRALRMHDRLVPWLVATN
                     LLGLFGNLASRHGWSTASALALLGQLLASVVLFQIANVHVQMQRLNPLWRVPFSLFLG
                     WLFVKALAHVGPVLAPLGGEGLPNSLFWPTLVLAALGATSWFIAAEAEEGIVSAVLAW
                     ACVAISIENFRFGSVFVGAVALFVGLGSGLIALATESQRDWRRFTLLIKTRSALLAAK
                     RPHVGAPARDASRSPELARADIGE"
     gene            complement(4526..5311)
                     /locus_tag="VFQ61_10210"
     CDS             complement(4526..5311)
                     /locus_tag="VFQ61_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954869.1"
                     /translation="MAIKHLTAPLLAGGVLLGYAAWRAQSSRAKRARTGSSSRHASET
                     SDSPLAGDTAVSTDIPPESARERRSAIENVVFDPETLYDSANLQENPASEEAIMQDEA
                     MLQGIFDVPELDPEREIPPARFARDGVPSRSGFFSEPTTLRNEPKPESSPLVDFASRT
                     NDTIEHEEYEQYQAEGAMAPEDIGVRWLARATEALSPYHTAFTRSHALKEDWVEAGLV
                     AQASMRAADGGELEFDLDDLEEILIEEIPESAVPTNRFGSPRR"
     gene            complement(5375..5635)
                     /locus_tag="VFQ61_10215"
     CDS             complement(5375..5635)
                     /locus_tag="VFQ61_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET9954870.1"
                     /translation="MSIILFLVFGFIVGLLARALMPGRQSMGMLMTTLLGVVGSFVGG
                     LIGSFISGNSVDRVHPAGLLGSVIGALIALAIFVSVGRRRTI"
     gene            5922..7661
                     /locus_tag="VFQ61_10220"
     CDS             5922..7661
                     /locus_tag="VFQ61_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954871.1"
                     /translation="MRWRSLARGAAWCVAGVVGLELLYVVGANAVLHTSLISNAVNAV
                     EGMHFEYSGAYSLYPGRVHVTGYQHRVETYDVEFELTIEHATMNVRLLELASRKFHVD
                     RLQADGVAFRMRHKVHSVGRNGERLKAYPPIEGFKDPPLYQGDRTPATPDADYHSWQV
                     GIRNVSARVRELWILEYRYRGEGVATGSFVLQPERFLILEPATLKLGPGKVTAGPVLA
                     ARKASGELKCAVPYLDFRKTKGLEVLHGIVAKADVTWEDGDLGFLNLYTQPSLGVTLS
                     GSSHLKVSWTADKAVVPPRSQVVLDSPDATGRSDRFTVKGPLHATLERPSDAEQLVAL
                     VELNGAKVSHASSKRPSPRLERFNARAVLGGVDLAKPIELAEASLRAKANIPSLSWFA
                     AIADPSAARHIEGQGDVSLEFERDERGTGKGEAVLVLDDVVLRSGEDEAKTAGLLSTR
                     FKTQEQPRPFLQGGLSVKLAQVNAPDLLPGIAKLAADALTARELNANVAYHAASTGLT
                     LEMTQASWGALRGRGFFFAGTGRDSAGALLVSAGPARVGVRMRDGQLESSPLVGSDWL
                     DRVWDAQRGMVKD"
     gene            complement(7761..7958)
                     /locus_tag="VFQ61_10225"
     CDS             complement(7761..7958)
                     /locus_tag="VFQ61_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET9954872.1"
                     /translation="MNWDLVEGKWKEIKGQVRSKWAKLTDDDLEAVAGRKDMLVGRLQ
                     QRYGLKRDAAEAEVDTWIKEV"
     gene            complement(8002..8355)
                     /locus_tag="VFQ61_10230"
     CDS             complement(8002..8355)
                     /locus_tag="VFQ61_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET9954873.1"
                     /translation="MQNRNLLMSLGTAIAASKVAKTISGLELEDVLGTVGLSRRRSHV
                     LENIAFVGLGVLIGAGVTALFAPMSGRETRQRIGEEATKFGQAAKQAVIEQKDDALRS
                     LSTVANGAVANQSRS"
     gene            complement(8589..9143)
                     /locus_tag="VFQ61_10235"
     CDS             complement(8589..9143)
                     /locus_tag="VFQ61_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954874.1"
                     /translation="MSLKPQLTYFLGGALSFALALTIAKRFQKTSQTLPEQHSPENTD
                     RIGGGWSDKSLSVSALDASGWTHAEVVDVDAVDEDELPHELVIDSSVVEPGFVDTSFA
                     AADQAEIANSEADPQAALAPEDLGVAWLSRATQTTGTGVPAAPEGADDQDSLEDVTDP
                     SGRPTDPMLGQVFATRFVREGNPR"
     gene            complement(9186..10169)
                     /locus_tag="VFQ61_10240"
     CDS             complement(9186..10169)
                     /locus_tag="VFQ61_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9954875.1"
                     /translation="MKHLSILSGLALLAITACGSNAPSPALVDARRAVDQARQSDAQR
                     VAPDRVLSAQQALDAAERAHAEDAGSLDERSLAYVALRKAELAEAYGSLLQNEQRQQQ
                     AQQSYVSTQDRLRREAENRADYTQRSLERTENALAQTRGAVDQERAARMEAEKRAGAA
                     LQSLREIAQVKEEARGNVILLEGSLLFATAKSELLPVARQKLDQVADALKQVDSDQRI
                     LVEGYTDSRGADDANQRLSEQRAESVRSYLVSRGVKADMISSVGRGEQQPIADNNTAE
                     GRASNRRVEIIVQPKGAGQPSSTQQSNSQNAPGSQSGTGTPATQNTQGTTR"
     gene            complement(10228..10572)
                     /locus_tag="VFQ61_10245"
     CDS             complement(10228..10572)
                     /locus_tag="VFQ61_10245"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HET9954876.1"
                     /translation="MLGRAISVLVVGSFVACASQGQAPSVTDAHSAISAAQAVGAERE
                     PKAALHLKLARDQTAQAEAKIRSGDNHEAKLLLDQAKVDAELALVLTREAAARAQAEQ
                     ALQKVQQLQQNQ"
     gene            complement(10948..11580)
                     /locus_tag="VFQ61_10250"
     CDS             complement(10948..11580)
                     /locus_tag="VFQ61_10250"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HET9954877.1"
                     /translation="MPPRSPATSGSVTNSTAGAALPPPQTPSSAVAPVVTTAVGAPTS
                     SDSREVGTGERYAGWRATIQRWISNSARRAGEPATAAIAMGLLAFTASMGMIVGFSER
                     WQLMINTCALFVTFGMVFLIQLTQARNNREIERKLDLILRGSGVTDLDLASLPEAQLV
                     SLDARIRQLTAGRLQDAYDDQGPTQVLDRQVVDRSADSAVRRSPVTRRAG"
     gene            complement(12067..12231)
                     /locus_tag="VFQ61_10255"
     CDS             complement(12067..12231)
                     /locus_tag="VFQ61_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HET9954878.1"
                     /translation="MLYWTVVLFVVALIAAIFGFGGIAASAAGLAKILFFVFLVLAVV
                     SLLLGRRAPT"
     gene            13051..13836
                     /locus_tag="VFQ61_10260"
     CDS             13051..13836
                     /locus_tag="VFQ61_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9954879.1"
                     /translation="MHHTALEMQRNRKEQDVKDHGTNGKSKAAEVARHSIEEALRELA
                     ATRDEAKVQAHLLSLEAKQRLAQVESSLRQFEQRLMDTSSPAEGLISRAKEFTHVLRD
                     LLGESANKATLSTPVRVLMSRGVRTCSEHDSLKAAAQIMWEADCGAVPVVSDGNRLVG
                     IITDRDVCMAAYLQGVDLSHAQVGSAMSRDLAVVGPDEPARVATEIMRRKRVRRVPVV
                     ERDGILVGLISLADLVRLAEPSQTWLTETLSRICEPNREKLES"
     gene            13921..14232
                     /locus_tag="VFQ61_10265"
     CDS             13921..14232
                     /locus_tag="VFQ61_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954880.1"
                     /translation="MKNSILSSGVLAITLSVAALSIVGCSAADKVENKISCNSVCNRY
                     KDCFDSDYDVDACSDRCEAETNADEDKDRRLEVCKACIDDRSCSSATFNCATDCAGVI
                     N"
     gene            complement(14402..15949)
                     /locus_tag="VFQ61_10270"
     CDS             complement(14402..15949)
                     /locus_tag="VFQ61_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954881.1"
                     /translation="MKLRSSSERARVLNRVQIVGHRIDSNQGVLGRTARRVRLSSFAA
                     GAAVLLGAVSQQACSGEVGAEPGTGGGVSPGNLGGSGGVLAAGGKAGASGGSTGGRSG
                     NGGAPGVGGNQSAGSSAKGGSVATGGSSSGASTGGASGGASNGGAAGTPTGGSSSGGA
                     SGAGKKAADIAKRLGRKPNFLIGMGNDLDNDHSKDGAYTLGVTMDLHYAYMVGLKGLG
                     GWPDWNAEGTFVNILADSAKAKGVTPMYTLYSMAAQGENNAAALTDNSYMQAYWDGAK
                     LLFQRLGDFNGPALVQFEPDFWGFMQQTSKSNPSNQKVNVSALAPDCAALPNNLVGMG
                     KCLVTLGRKYAPKAVLAFEVSQWADPDPVKVAKFLLEVGASDADLLTTDILDRDAGCF
                     EAHTDPACQRNDGPWYWDETNKTSPNFHEYLAWIKKATDAAGLPILWWQTPFGVPSST
                     PGGTDGKYRDNRVKYIFEHVQEFIDAGGVGATFGVGSGNQTYITTDGGQFKNAVTKYY
                     ASPVPLP"
     gene            16093..16686
                     /locus_tag="VFQ61_10275"
     CDS             16093..16686
                     /locus_tag="VFQ61_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954882.1"
                     /translation="MKECVVEVIRVDRARTRLGTISGHRITRAGPSPELTARAAEGGA
                     RLILVKPYRARHRALEHDTLAELSMTPHDESNLNRPRSAASAASSEVASAAALATPAP
                     RSSPVRRTALELAIGLVAGVVGASVFGPSMISFWYEPPSKDAFSCASSVEQALKDFLK
                     LQLGLALVGALVLVLASFLVRRFFRQRRERRSSNTGA"
     gene            16826..17254
                     /locus_tag="VFQ61_10280"
     CDS             16826..17254
                     /locus_tag="VFQ61_10280"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET9954883.1"
                     /translation="MPTASSEHEYPVLIVERHLDTFGHVNNATYLELFEEARWDLIVA
                     NGYGIEKIRETRKGPTILEVRLEFKRELLNRQRIVIRTRIESYSGKVGTLVQVMQNDA
                     GDLFCVGTFLIGLFDLDARKLILPTSEWRKAVGLELSERP"
     gene            17295..19397
                     /locus_tag="VFQ61_10285"
     CDS             17295..19397
                     /locus_tag="VFQ61_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET9954884.1"
                     /translation="MTAEIPPRPATLTEKILARHALGPLPSVITPGASLRLRVDFTLA
                     SELAWNGMNRTYERLGRPDLYDRERFYIALDHTVDPQSLAHDPKTQRLVQLARQLRDE
                     HRLPHFFEPNTTILHTTFYRDFVQPGQLILGADSHTTSHGALGAFAIGLGGADVLAAM
                     LSGETWLEVPQCLEVRYVGSPSFGVLGKDMILATLQQLGRNTLALERAVEFTGDVMGL
                     SLDTRFAICNMTAELGGLCGLFEADARARAWLATRPRSFDEAVYERADAGAEYAARVR
                     VDLTGLLPKLAEPFSPDNVVDVAERTGLALDGCFVGACTTTEEELVLAGLLLERAWAS
                     GMRPEARDSGRRLLVPGNREIASKLQESGLLTSYERAGFVVDPPGCSMCLGVASRRAG
                     AGETWLSSQNRNFPNRMGAGSLAYLASACTVAASAFEMKLRDPSRWFEQIDRARLARI
                     LAGGPGGAAPQVTSAKFATERSPLQLQQGNRAVESADSSLPQATQGSASISEVAADDS
                     AAVFGKAQRRVTTRLQGRVQRFGDAVDTDAIIAGEFCHLQEPAELGRHCLKYVRPDFV
                     TRVGQGQNIVVAGQGWVSGSSREQAVWALQGAGVQLVIARSFAFIHKRNLVNEAFPFL
                     ELADPAFYDLAVEGRELSVDFDSGEVLDVESGQVFSGLPPRGAARRLFEAGGLIATFE
                     RNEGSSTRFQSGFSRTSK"
     gene            complement(19367..19852)
                     /locus_tag="VFQ61_10290"
     CDS             complement(19367..19852)
                     /locus_tag="VFQ61_10290"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9954885.1"
                     /translation="MQDIVALVESAYRGDSSRSGWTTEADLLDGQRTDVAEVEALIAA
                     TASRVVIARRAGDLVACVCLKSAAEHVYVGMLAVQPRLQGHGLGRALLTEVENIVRRE
                     YLPPKLRMTVIAQRSELIAYYERCGYRRTGEREAFPYGEPRFGLPRRADLYFEVLEKP
                     L"
     gene            20128..20262
                     /locus_tag="VFQ61_10295"
     CDS             20128..20262
                     /locus_tag="VFQ61_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954886.1"
                     /translation="MTTSNEKRRSATAHTRENVYDVADRTGWPELYRTSHGPGLFSEP
                     "
     gene            20252..21067
                     /locus_tag="VFQ61_10300"
     CDS             20252..21067
                     /locus_tag="VFQ61_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954887.1"
                     /translation="MSHEPDPNGASGAGAAPAGGNTATSSSSSSGGVVHQGGGTQSGG
                     TQSGGTTGSGTQPPPDPLALHQSCAGTASGGVLDSIEATVRWINAQPKPLTLACFLQS
                     LQKPLKLNATYSIVSAQPSMGTRSPRLFIFFDGLILSVVPEGTGSSLLELGQLDGETR
                     TVKAEIQFPVTQTLAPEAPFERVLYMQAFATVCGGCHGSEARYESIAYAKAYVSKAIR
                     PEPSYAVPLSTLQRELAVCDDTLEFDRCEMLRALQQGGPISQDFPPGMSTSFF"
     gene            complement(21260..22732)
                     /gene="ahcY"
                     /locus_tag="VFQ61_10305"
     CDS             complement(21260..22732)
                     /gene="ahcY"
                     /locus_tag="VFQ61_10305"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET9954888.1"
                     /translation="MSEIITYKKAPVAKDGKSDYVVRDLSLAAFGRKEIDLAETEMPG
                     LMATREEFAQRQPLKGARIAGSLHMTIQTAVLIETLEALGAEIRWCSCNIFSTQDHAA
                     AAIADRGVPVFAVKGETLEEYWDYSWRVLQWSDGGTPNLILDDGGDLTLLVHLGVKAE
                     KDASFLNEDPGHEEGRILYALVKRAIKERPGFFAQAAKNIRGVSEETTTGVHRLYQMA
                     QKNELLFPAINVNDSVTKSKFDNLYGCRHSLPDGIMRATDVMLSGKVVVVAGYGDVGK
                     GCAQAMKGQGARVLITEIDPICALQAAMEGYEVTTMNKAAPRGDIFVTTTGNEFVITE
                     EHMNAMKHRAIVCNIGHFDSEIEIANIRKHRWIEVKPQVHEVVWPNGKSIIVLAEGRL
                     VNLGCATGHPSFVMSNSFTNQTIAQVDLWLAAQGKPTLSGIGGYERGRVYTLPKKLDE
                     KVARLHLAKLGVELEELTDRQAQYLGIPKEGPYKPEHYRY"
     gene            23749..23919
                     /locus_tag="VFQ61_10310"
     CDS             23749..23919
                     /locus_tag="VFQ61_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954889.1"
                     /translation="MPDDIHLSNSSIRAEQSVKIATLINSHEIGPGQLPENSTVAGCS
                     QTGSRPKPKLKQ"
     gene            24128..25189
                     /locus_tag="VFQ61_10315"
     CDS             24128..25189
                     /locus_tag="VFQ61_10315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9954890.1"
                     /translation="MGQTIEPTLGYSARNGTAVSSTARINASDAGQGPAQTQIRKRRV
                     SVIIPTRDRPDFLAEAMTSVRALEGPDLEFEILIGDNGSNPDTPKVAERFGARYFKVE
                     RQGAAAARNAGMRAATGDYIAHLDDDDLWTADHVRPHLALMEANPEIYTVVGRILCTD
                     TERKPIGEAWPQSVPDHGDLFMTFMSYFPQVGGTISRAAVRETVGLFDETLLGDADWD
                     WQLRIAKKHRIGFAPVTCVLFRQRPSGEADAVHLRRIPHTRRIFLRHALPEWRRWNSP
                     TGLYRSYNKSMAPYFIYFVNTAHARAERGDYRGALAAIGHAVKLVPVRGLREFARSSD
                     LRGSLFRAVLGGRLSQKTH"
     gene            25228..26028
                     /locus_tag="VFQ61_10320"
     CDS             25228..26028
                     /locus_tag="VFQ61_10320"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9954891.1"
                     /translation="MKQTIKGLLRRSGLRRSDVAAARMYCERQALARWGRRRPRQQGR
                     ILCYHSVGQPSFGVNDIPPQAFRQQLEWALKQGYRFVPAEKIARDGGDPRSLAVTFDD
                     AFKTVGTQAAPILAEYGIPWSVFVVTNWSDGGGGWAPETFLNWSELRELADKGAEIGS
                     HSVSHPDFGYLDGAHTRDELRRSREIMEERLGFAPHSFAIPFGQSKNWSPVATEAARE
                     AGYRIVYAQAEGSRPMFTVPRTFITHYDNGRVFEAALQGVFDHWEEWV"
     gene            complement(26160..27275)
                     /locus_tag="VFQ61_10325"
     CDS             complement(26160..27275)
                     /locus_tag="VFQ61_10325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9954892.1"
                     /translation="MLLYTNTRFVGGMEEHIELIARHLDRRRFEVFSICPALDAVKPF
                     AENLQRYSDHFAAITFDRFHLQNWPVLMQQIRDWKIDVMHMHNGYYRGQNLSYLAAFA
                     AGVRKVYITEHLAPEQREMRHHELLRNFTARIASGIICVSEKNYLARKEFLYTPPDRT
                     FIVKNGVDLDDFTPIPEPTLAELRARYSLPEDAEVVGTMVRFESNKGLEYLVDAMVKI
                     RAARPKAYLLMVGDGSLRGELEAQVERVGLKEFTRFTGFQTEPRPFFGLMDAFVLPVP
                     IGSMSIALLEAMAMSRAVVITFGGKGEAVIHEQTGLCAEPRNSSSIAESVIRILESKS
                     FKEQLGQAARKHVEEEFSAATTARRLEEIYQRNLTRS"
BASE COUNT         5341 a         8620 c         8548 g         5180 t
ORIGIN      
        1 gtccaatcgc tgatcaacaa ggcgccatcg aagcggctcg ggaaagcgaa gcgcgccccc
       61 gcgggcgcgc gatacaccgc ccccgcgatc gcggcgcgcc ccacgaccga gccatccgtg
      121 agcagcccgg gaccgagcgc cggataacgg ggcgtgccgt agtcgtaagc catccaggcc
      181 gggatcgtcc cttcgcagtc gtacggagcc ccaatcgact gattggcgaa gtcatagttt
      241 gcgtaaggca actgctcggc gatgcaataa ggccaaccgt agttgcccgg ccgcgcccag
      301 ttgatctcgt catagccacg tgggccgcga tcgctatccg tcactgcgtc gggaccgatg
      361 tcgctgaagt acacgcggtc gagcttggga tccgcggcca aacggtaggg gttgcgcacg
      421 cccaaagcgt aaatttcctt gcgaccgtcc cgttcgtccg cgccgaacaa gtttccctcc
      481 ggcacgccgc catcgggacg gatgcgcaag atcttgcccc tcaaatcgaa gggattggct
      541 gacgtgcgct gcgcgttgaa ggtctcacgc ccggtacgtt cgtcgagcgg cgccgcgcct
      601 gcagaatcga agggattggt gttatcaccc gtcgcgagcc acagcgtgcc gtcggccgcg
      661 aaggccagag acccgccttc gtggcagcag cgacgttcac tcggtacctc gagcaagatc
      721 tggcgagaag cctcgtccaa atggccatcg gctcgcgact tgaagcgcgc caactcgttg
      781 cgtccagggg gcccgcccgc aacatcctca ccggagagcg gcgccgagta atagagataa
      841 acgaagccat ttttcgcgaa cgaggggtcg agcgcgagcc ccagcagtcc gttctcctga
      901 gcggtgtcca cctcgacgcg aaatgccacc tcgacggcac cggtgcgagc ccgccagagc
      961 ttcagctccc ccgtacgttc gatgaagtac acgtcaccgt ccgggcgcac cgcgagagcg
     1021 attgggctct tggcattacg ggtgaggacg gctcgcccaa aaccgcctcc gcgcgcggcc
     1081 caacgaatgc cctccaggat gtgctgctgg aagcgcgtgt catgccaagt ctgcggccga
     1141 tgtccgagat tcgtgtagaa cgaacgcgcg ccttcgtatt gctggtacca ggcgaccggg
     1201 tgatccgcac ccatcgaagg gcctggcgga gtattgggta cggtgaaacc gcgctcgtcg
     1261 atgctgagca ggacggtcgc cacttcacgc gggttgcggt cgaaattgta ccactcgtcc
     1321 tcgaagcgaa actgcggagt aaagggctgg accgcaggat gcgtgccctc ctccacatgg
     1381 acgtctacct ccagggggga ttccggatgg ctcacgaacg tcgcgcccac cagcccgacg
     1441 taccagggcc accccgcctc ggtatcggca gccgcgtgga tccccacgaa gccatggccc
     1501 ccgcgtaaat atcgctgaag tgcggcctgc tgggcaggag ccagcacgtc tccggtggtg
     1561 ttggcaaaca ccaccgcttc gtaagcgctc aacgtggcgt catcgaacac agccgaatct
     1621 tcggtcaggg tcgcgctgat ccgctgctcg gcgggcagct cggagaagaa ttcctgagca
     1681 tcggcgatcg agtcgtgtcg aaagccctct gtcttcgtga acactagaat tcgaatcgag
     1741 ctcggcaact ctggagagtg gtccggcgaa ggctccgccg gctctgtgct gtcgacgtcc
     1801 cgacaagcgg ataacccaac gccgagactc gtcagcagta tcgaaaccgt tcggagcggc
     1861 gcattccaga gtttggctct ccacgtccgg ccacgagccc gtgctggcgg aggcagcatc
     1921 gaacctttac ctacgcagag aaaccgccga acgggctcga gagactggcc agaagcagcc
     1981 ggacgcaaat cactcatcac aactcgcttt cacgccgagc gtcctagaaa gcaaggggtt
     2041 tcgctccgaa gcttgacctc gcaggcgcct tcgcgttctg tgttcgcctt gaacgactca
     2101 gcccgaactc ccgcccaaac tggttcaact caagcaggtt ctccagtaac cccagcaaac
     2161 gcgcggcaag gagcgaacgc acgtgacggt gagagtgctc cacccgcaag caacgtggcg
     2221 gcccaagcgg gcgcatcggg tcagccgagc ttgccgctgt cggaagacgg gcgagtgcca
     2281 cccgaccggt tgacgctcgg caccaagctc gcatacgccg tcggcggcac cagcgatatc
     2341 tttggacatt ggctttacaa cacgctggtc gaccccgttt acaatgtatt cttggggctc
     2401 gcgcccactc agatcagcat cacgcgtgcg ttgactctgc tggtggacgc ctgcagcggg
     2461 ttgttcttcg gttggctctc ggacaatacc cgaacccgct ggggccggcg gcggccttat
     2521 gtgctggtgg gctcgatttt ggccggagcc ggcctgccgt gcctgttttt ggctcggccg
     2581 agctggagca gcgaggcgac gttctggtac atgctcgtct cggcctgcct ctacgctccg
     2641 ctgattgcgt gtttcaacac accttttcaa agcctgggcg ccgagctaac gccggactac
     2701 aacgaacgaa cctcggtgat gtcctacaag ggcgtgattc agaagatcgc cggggcgctg
     2761 atcacgggtg cgttgtggtt cgccacccga ccgatgttca acaacccggt gacggggaaa
     2821 ccagacgttg cacgtggcgc catgtgggca gcggcgcttg ccggtggctg gatggcgttc
     2881 tccggtatcg tcaactttgc gttcgtgccg gagcgctact actccaaggc gaaacaacaa
     2941 gcgcgcgcca gcttcatcgg tatgtttcgg gatgcgttga gttgccgccc ctatctaatc
     3001 ttgttgggcg cggccctgat gtacgccgtg ccgaccggac tggtcggcac cctgggattt
     3061 tacgcgctga cttaccacgt gttcgcgggc gacatgacgc gcgcatccac gctcttgttc
     3121 tggggcggca tcgcatacac cgtactcggc gtgatgggca ttgccgccgc caatcacatg
     3181 gctcggcgct tcggcaagca ccgcacgctc attggtgcac ttctgattgg actcgtggcc
     3241 ttcgcgtcga gcttttggct ctacaccccg acgtacccgt acctgagtat ggtaacggga
     3301 gggctgaacg gtttctccgc taccggcctt tgggtggtgc tcccggccat gacggcagac
     3361 gtggtcgact acgacgaact gcacagcggc aagcgtcgcg aaggtgcata caccgccaca
     3421 ttttcttggg tcatgaaggt aggaatgatg ctcagcatgt tgatcggcgg acccttgctg
     3481 gagctgaccg ggttcaacgc caagttgggc ggagcccaag cgccgagtac gatcctgtgg
     3541 attcggatcc tcttcgcagg aattccggtc acggctctgg ttttggcgct ccttctcatt
     3601 cgcctatatc cgctcgggca agctcgggtg accgagatgc gtcgcgagct ggagcgccga
     3661 cgtggggccg tttagcggcc agaagggctg agcgcgtctt aatcaagagg gtgaaccggc
     3721 gccagtcgcg ctgcgactcc gtcgcgagag cgatcaatcc gctcccaagt cccacgaata
     3781 gcgcgacggc accgacgaag accgatccaa agcggaagtt ctcgattgaa atcgccacgc
     3841 aagcccaagc caagacggca gacacgatgc cctcttccgc ttcggccgcg atgaaccacg
     3901 aggtcgcgcc gagtgctgcg agcactagcg tcggccaaaa cagggaattg gggaggcctt
     3961 cgcctcccag tggggccaaa actggtccca catgcgccaa tgccttgacg aagagccaac
     4021 cgagaaacaa cgagaacggc acgcgccata gcggattgag ccgctgcatt tggacgtgca
     4081 cgttggcaat ttgaaacaga accaccgaag cgagcagctg ccccagtagc gccagcgccg
     4141 atgccgtgga ccagccatgg cgggacgcca agttaccaaa caggccgagc aggttggtgg
     4201 caacgagcca cggcacgagc cgatcgtgca tgcgcaaagc gcgctgatcg gcgcgcagcg
     4261 cgctcgagcc gtaggcgatc agggacgcat acacgggaag cgaaatcgcg aaggcataag
     4321 tcgcaggccg aagcagggtc ttggctgccg aagagcctgg cgcgagaaag tcaccacttg
     4381 caaagcctaa cgcggcgctc gagattgtcg caaggagccc aatcgcacgc caagtgctgg
     4441 gcccggtgct ctgagcgccg cgtccgaatc taagatgtgt ctctaatagc gaaatcaacg
     4501 ttcgagtgca gttggttaca tccggctagc gccggggtga gccaaatcga ttcgtgggta
     4561 ccgccgactc tgggatctcc tcgatcagga tctcttctag atcatccagg tcgaactcga
     4621 gctctccccc gtctgcagcg cgcatcgagg cttgagcgac taggcctgct tccacccaat
     4681 cttctttcaa agcatggctt cgcgtgaaag ccgtgtggta cggagacaac gcctccgtgg
     4741 cgcgagccaa ccaacgtacg ccgatgtcct ccggcgccat cgcgccttcg gcctggtact
     4801 gctcgtattc ctcgtgctcg atcgtgtcgt tggttcgtga cgcgaaatct acgaggggac
     4861 tcgactcggg ttttggttcg ttccgaaggg tcgttggttc agaaaagaac cccgaccgac
     4921 tcggaacacc atctcgagcg aatcttgccg gcggtatctc gcgctccgga tcaagctccg
     4981 gaacgtcgaa gattccctgc agcatcgcct cgtcctgcat gatcgcctcc tccgaggcgg
     5041 ggttctcctg caggtttgca ctgtcgtaca gagtttccgg gtcgaaaact acgttctcga
     5101 tggctgaccg tcgctcccga gcggactccg gcggaatgtc tgtggaaaca gccgtgtcac
     5161 cggccagcgg agagtcagac gtttcggaag catgacggga tgatgaccct gttcgtgcgc
     5221 gcttggcacg cgacgattgg gctcgccatg cagcgtaacc cagcaggaca ccgcccgcga
     5281 gtagcggggc ggtcagatgt ttgattgcca tgcgcgcgtt ccctgaaggg caagacctaa
     5341 gtcgcaattc gagtcgagat tcgcactcgg cacatcagat ggtacggcga cgaccaacgc
     5401 tcacgaaaat cgcgagagcg atcaaggcgc caatcacgct gcccagcaag cccgcgggat
     5461 gcacgcgatc tacgctattg ccgctgatga agctaccgat caagccgccg acgaaggagc
     5521 ctacgacgcc cagcagggtc gtcatcagca tgcccatgct ctggcgccca ggcatcagcg
     5581 cgcgcgccag aagaccgacg atgaatccga aaaccaggaa taggatgatg ctcatgcctg
     5641 gacactgagc aactcgcgtg ccgagtgctt cgatcgcgcg ccgagcggaa cacgcaggaa
     5701 tgagccgcgc gccgcagctc gttcttccac atctcgcaga cccttcagga gcccgtctta
     5761 gtaaaacggc ggagcaaagt gccctttcat gtggcgcgtt tgttgccacc gcactgccag
     5821 catgcgacgc cacgtcgata gcttcatagc tcccggagta ccgcttgaac tcgaaccttg
     5881 accagcggct acgcacgatg ggctctgcaa ttcgccgcac tatgcgctgg agaagcctgg
     5941 ctcgcggcgc ggcgtggtgt gtcgcgggcg tcgtaggact cgaacttctg tacgtggtgg
     6001 gcgccaacgc cgtcctgcac accagcttga tttccaacgc ggtgaacgcg gtagagggca
     6061 tgcacttcga gtactctggt gcttatagcc tctatccggg ccgcgttcat gtgaccggct
     6121 atcagcatcg agtagagacg tacgacgtcg agttcgagct cacgatcgag cacgccacga
     6181 tgaatgttcg actcctcgag ctcgcctctc gcaagttcca cgtcgaccgc ctgcaggcgg
     6241 atggtgtggc ctttcggatg agacacaaag ttcactcagt ggggcgcaat ggcgaacgac
     6301 tcaaggccta cccgcccatc gagggtttca aagatccgcc gctgtaccaa ggcgatagaa
     6361 cgcccgccac acccgacgcc gattatcact cctggcaagt tggcattcgg aacgtttccg
     6421 cgcgcgttcg cgaactctgg atcctggaat atcgttatcg cggcgagggt gtagcgacgg
     6481 gctcgttcgt gctgcagccc gaacgctttc tcatcctcga accggcaacg ttgaaactgg
     6541 gtcccgggaa ggtgaccgcc ggtccagtgc ttgcggcacg taaggcgtca ggtgaactga
     6601 aatgcgcggt cccttacttg gacttcagaa agaccaaggg gctcgaggtg cttcacggga
     6661 tcgtggcgaa agccgacgtg acttgggagg acggtgacct cggctttctg aatctctata
     6721 cccagccgag cttgggagta accctcagcg gctcctctca cctgaaggtg tcttggaccg
     6781 ccgataaagc cgtggtacct ccgcgctccc aagtggtttt ggacagcccc gatgcaacgg
     6841 gtaggtcgga ccgattcacg gtaaaaggcc cgctccatgc gacactcgag cggcctagtg
     6901 atgccgagca gctggtcgct ctcgtagagt tgaatggagc taaggtttcg cacgcgagtt
     6961 cgaagcggcc gagcccgcgg ctcgaacgct tcaacgcccg cgccgtgttg ggcggagtcg
     7021 atttggcgaa gccgatcgag ctcgccgagg cgtcactgcg agccaaggcg aatatcccca
     7081 gcctctcttg gttcgccgca atcgccgatc cgtcggcggc gcggcacatc gaaggacaag
     7141 gagacgtcag cctggaattc gagcgtgacg agcgtggaac gggcaagggc gaagccgttc
     7201 tcgtgttgga tgacgtggtt cttcgctccg gtgaggacga ggccaaaacc gcaggtctgc
     7261 tgagcacgcg gttcaaaact caggagcagc cgcgaccctt ccttcagggg ggtttgagcg
     7321 tgaagctagc tcaggtcaac gcccccgacc tgctgccagg cattgcgaag ttagctgcgg
     7381 acgcgttgac tgcacgggag ctgaacgcca acgtagcgta tcacgcagca tccaccggcc
     7441 tcactttgga gatgacccaa gccagttggg gagcgctccg cgggcgtgga ttcttcttcg
     7501 cgggcaccgg ccgggactcg gctggcgctc tgctcgtgtc cgcgggcccg gcccgagtgg
     7561 gggtccgcat gcgcgacggc cagctcgaga gctcgccact cgtgggttcg gattggctgg
     7621 atcgagtttg ggatgcgcag cgcggaatgg tcaaagattg aacggctgtc ttctgccttc
     7681 cgcgcagaca aaaacgaacc gcccactctt cggagtgggc ggttgatcct cacgctgatt
     7741 tccgagcagg gctcagagac tcagacttcc ttgatccacg tgtcgacctc ggcttcggcc
     7801 gcgtcacgtt tcagcccata gcgttgctgc aagcgcccaa cgagcatgtc cttgcgcccg
     7861 gctaccgcct ctagatcgtc gtcagtgagc ttggcccatt tcgatctgac ttggcctttg
     7921 atttccttcc acttaccttc gactaagtcc cagttcatga tgccctccgt tgaactgctg
     7981 aggctagaga atgttggagt ttcaggagcg cgactgattc gcgactgcgc cattggcgac
     8041 cgtggagagc gagcgaaggg cgtcatcctt ctgctcgatc acggcctgct tggcggcctg
     8101 accgaacttg gtcgcttcct cgccgatccg ttgacgagtc tcacggcctg acatcggcgc
     8161 gaatagcgcg gtcacgccag caccaatcag cacacccaag ccgacgaacg caatgttctc
     8221 caggacatgg ctgcggcggc gcgaaaggcc gaccgtgccg agcacatctt ccagctcgag
     8281 accggagata gtcttggcga ctttggatgc tgcgatggcc gtaccaaggg acatgagaag
     8341 attgcgattt tgcatgatgg gtatgttcct tttctggtcg aactgactgc gagtggttgt
     8401 cagacgactt gtcacgtaca agccgactca ctgtttacta gcgatcgatc gtctctcgaa
     8461 tcgccgcact tgaagtggtt ggcttgcatc gacggcttac gcaccactca agcaccgcgc
     8521 actcaaaaag caccatccat gccaggccgc tccgacactc ccgaacaggg gagcgcctgg
     8581 tcatgggtct agcgtgggtt tccctctctg acgaagcggg ttgcgaaaac ctggcccaac
     8641 atagggtccg tggggcgacc ggacggatcc gtaacgtcct ccaaggagtc ctgatcgtcg
     8701 gctccctcgg gcgcagcggg cacgcccgtg cccgtggttt gtgtggcgcg cgacaaccac
     8761 gccacgccaa ggtcctccgg cgcgagcgcc gcctgcggat ctgcttctga gttggcgatc
     8821 tcagcttggt cggcagccgc aaaggacgtg tcgacgaagc ccggctcgac gacactggag
     8881 tcgatgacca actcgtgtgg aagctcgtcc tcgtcgaccg catccacgtc cactacttcg
     8941 gcgtgagtcc agcccgaagc gtccagcgcc gataccgaca ggctcttgtc ggaccaaccg
     9001 ccgccaattc ggtcagtgtt ctctggcgag tgctgctctg gcaacgtttg cgatgtcttc
     9061 tggaagcgct tggcgatggt gagcgccaga gcgaaactga gcgcgccgcc cagaaagtag
     9121 gtcagttggg gtttgagaga catgactggg ttcctgagtt gagagcagcc tcgagacgga
     9181 acagatcagc gcgtggtgcc ctgcgtgttc tgagtggcag gcgtgcccgt accactctgc
     9241 gaccccgggg cgttctggct gttggattgt tgagtactgc tgggttggcc tgcacccttg
     9301 ggctggacga tgatttccac gcggcggtta ctggcgcgcc cttctgcagt gttgttgtcg
     9361 gcgatgggtt gctgttcgcc gcggcccacc gaagagatca tgtcggcttt gacgcctcgg
     9421 ctaaccaggt atgatcgcac ggactcggcg cgctgctcgg agagtctctg gttggcgtcg
     9481 tcggcacctc tcgaatccgt gtagccttcg accagaatcc gttgatcact gtcgacctgc
     9541 ttcagcgcat ccgcgacctg atcgagtttt tgccgtgcga ccggcaagag ctctgatttc
     9601 gcggtagcaa acagcagcga tccttcgagt agaatcacgt tcccgcgtgc ctcctccttg
     9661 acctgcgcga tctcgcgcag actctggagc gccgcgccgg cccgcttctc ggcctccatt
     9721 cgcgccgctc gttcttggtc taccgcgccg cgggtttgag cgagtgcgtt ctccgtccgc
     9781 tcgagggagc gctgggtgta gtcagcgcga ttttcggcct cacgccgcag gcgatcttgt
     9841 gtagagacat acgattgctg agcctgttgt tggcgttgct cgttctgtaa caaggaaccg
     9901 taagcctccg ctagctcggc tttgcgcagc gccacgtaag ccagactgcg ctcgtccaac
     9961 gagccagcgt cttctgcatg cgcgcgttct gcagcgtcca gcgcctgttg ggcgctcaat
    10021 acgcgatccg gagccacccg ttgcgcatcc gattggcgcg cctgatcgac ggcacgccgc
    10081 gcatccacca gagccgggct tggtgcgtta gagccacaag ccgttatcgc gagaagggcc
    10141 agaccagaca agatactcaa gtgcttcatg ggagatacct atttggattc gaaatcgtga
    10201 gttggattga acaaatcgag cggcgggtta ttggttttgc tggagttgct ggaccttctg
    10261 cagcgcttgc tccgcttggg ctcgcgcggc ggcctcacgg gtcaagacca gcgctagctc
    10321 tgcatcgacc ttggcctggt cgagtagcag cttggcttcg tgattgtcgc cactgcggat
    10381 cttcgcttcc gcttgcgcgg tttggtctcg ggccagcttc aaatgcagag ctgccttcgg
    10441 ctcccgctcg gcccctactg cctgagcggc gctgattgct gaatgggcat ccgtgaccga
    10501 tggcgcctgg ccttgagaag cgcaggcgac gaacgaaccg acgactaaaa ccgaaatcgc
    10561 acgacccaac atggaaattt gacctccaga aagtcggcaa cgtattcgcg gccggacgag
    10621 cacattgcgc gaccagcgag cgagtggcta ccggaatacc tgtcatcaaa agcaactcgc
    10681 atgcccgtaa ttcccgcaat cctttgagga atccgaatga cgaagtacgt gagttcacac
    10741 gctttccgac aggtcggatg cgcggctgca gcgcgacccg accgagggca gaaactcagt
    10801 cgtttcctgg ggcagacctt cgtccgacag gcacaaaaag ggacaacggg agccaggcgt
    10861 tttttcagcc tggtcccgtt gcgaagtttg tgtgggttta gaaggagagc cgcgcgtcgt
    10921 ctacgaatct cggaagcaac tttgatttca gcccgcgcgg cgagttactg gagaacgccg
    10981 gactgccgag tccgcggagc ggtctacgac ttgacggtcg aggacctgag taggcccctg
    11041 atcatcgtag gcatcttgca gacgccctgc agtaagctgc cgaatacgcg catccaacga
    11101 taccagctgc gcttctggca aggaagccag gtctaagtcg gtcactccgg agccacgcaa
    11161 gattaggtcg agcttgcgct cgatctcccg gttatttcga gcttgcgtga gctgaatcag
    11221 aaataccatg ccgaatgtaa cgaatagagc gcaggtgttg atcatcagct gccatcgctc
    11281 ggaaaacccg acgatcatgc ccatcgatgc ggtaaacgcc agcaagccca tggcgatggc
    11341 ggcggtcgcc ggttcaccgg cgcggcgcgc actgttgctg atccagcgct ggatggttgc
    11401 tctccaacca gcgtagcgct ctccggtccc gacttcgcgc gaatcgctcg aggtcggagc
    11461 accaactgcc gtcgtgacta ctggagctac ggcggagctg ggcgtctggg ggggcgggag
    11521 cgctgcaccg gcggtgctat tcgtaacgga gccgctcgta gcagggctac gcggtggcac
    11581 aggaactctc ggcagagcgg gagcatgcac cgcgccaagg ggcgccggac tcgcggccac
    11641 aggagcggca accgcgcctt gcggcgagac cgccgggagc ggcggcatcc ctttgaccgg
    11701 aagcgtgcgt tggccggcgt gtgaacgaga ggtcgaattc gggggggcgg ggcgaaagac
    11761 agggggtgag ttcaaagcaa caaccgttcg gctaagagcc accaatttgc atcggtggtg
    11821 gggtggagtt cgaatgagga gtattcaggt tcgaaataga ctccgaaagg ttcgaatgcg
    11881 gagccccgag agaacgaccg cgggtgcggc gggacgctcg gctgcggagc tctctcgctc
    11941 tgcagatcga acgccccgga cacccgcgcg tggagccgcc ggcgccggga tgaaagcggg
    12001 ccgaggggga aaggggggca ttctcaaggg catctgagct ccggggccgt tccacgcggg
    12061 tcagcttcac gtcggagcgc gacggccgag cagcagcgaa acgactgcca aaaccaggaa
    12121 tacgaagaac agaatcttgg ccaatccggc ggcactcgcg gcgatgccac caaagccaaa
    12181 gatcgcagcg atcagagcaa ccacgaaaag tacgacggtc cagtacaaca tgggaatgtc
    12241 tcctgatatg cgcgaagcgc aaatactcgt gatgagaagc gggttggccc gctcgggcta
    12301 cgcagccacg tatagcgaag gccatgccaa gcggagctcc gcgagccggc gcacctcgga
    12361 cgccgcgcag agcagttgcg gggcggagcc gccggcaacg cgagccgaag gagcctctaa
    12421 agccgaagca ctacgtaacg cacagaaaat ccgaaagtaa gcgcggccag tcttttcgcg
    12481 gtcacgccga gcatgcgcgt ctgaacggcg ccgcaacccc ttcgaccggg agcttcagca
    12541 acacgccatt gctcgctgta gcgaggcgta ctggcaaagc ggggctaatt cgtcggtcgt
    12601 cgtcctggta ccgcgcggtt ttccgatctt cggcggtccg acgggaaacg ttcacgatcg
    12661 ccccactgcg gcgaccacgc gaggtcaccc aggattcgcc ccggtcgcgg gatcacgagt
    12721 ccggtgaacg tgagtaagag gtggagctct ccaacagcaa cggcctcgcg tcgtgcgcag
    12781 caccggtgaa cgatgaagtc acgcgcagag ccccctgccc ccccttccct ccatgcaacg
    12841 caagctcacg ggcacagtgg agtcagaggt gttgagtcgg aggtgctgag caggatgtgg
    12901 tcgagagaga tgttcggcag atgttcggca gacattccgc aatgaccttc aggcaaacgg
    12961 cgatgttgca gtgctgctct gcgagcacta ctcgatcgga tgaacccaag ctgctcgtgg
    13021 ggctctctgc gacgcggcat ggcacgagcc gtgcaccaca ctgctctcga gatgcagcgc
    13081 aacagaaaag agcaagacgt caaagatcat ggcaccaatg gcaagtcgaa ggctgcagaa
    13141 gtcgctcgcc actccataga agaagccctc agggaactag cggcaactcg cgatgaggcc
    13201 aaggtgcagg cgcacctcct atcgctcgaa gccaagcaac gcctcgcgca agtcgagtct
    13261 tcgctgcgtc agttcgagca acgtttgatg gacacctcga gtcctgccga agggctaatc
    13321 tcgcgtgcga aggagttcac gcacgtgctg cgcgatctct tgggagaaag cgccaacaaa
    13381 gccacgctct cgacccctgt acgcgtactg atgagccgcg gagtacggac ttgctcggaa
    13441 cacgattcgc tgaaggccgc agctcagatc atgtgggaag cagattgcgg cgcggtgccg
    13501 gtggtctcgg acggcaatcg cctggtcggg atcatcaccg accgcgacgt gtgcatggcc
    13561 gcgtacctcc agggcgtcga tctctcccac gcgcaggtgg gatcggccat gtcgcgcgat
    13621 cttgcggtgg tcggtcccga tgagccggct cgagtcgcca ccgagatcat gcgtcggaag
    13681 cgtgtacgtc gtgtgcccgt cgtcgagcgc gacggcatct tggtcggctt gatttctctc
    13741 gcggatttgg tgcgtctcgc ggaaccctct caaacttggc tgaccgaaac gttgtcccgc
    13801 atttgcgagc ccaatcgaga gaagctcgag agctgacggg cacgctggat ccagaaaagt
    13861 cttagcggct tgcgggctga ccgcgagctg cttggtcgta cccattcgga gaaacaaact
    13921 atgaaaaatt cgatcctatc ttctggcgtt ctcgcgatca ctctttccgt cgccgcccta
    13981 tcgatagtcg gctgtagtgc cgccgacaag gtcgagaaca agataagctg caacagcgtc
    14041 tgcaatcgct acaaagactg tttcgacagc gactacgacg tcgatgcttg cagcgaccgc
    14101 tgtgaagccg aaacgaacgc ggatgaagac aaggatcgtc gactcgaagt ctgcaaggca
    14161 tgcatcgatg atcgctcatg ctcgagcgca acgttcaact gtgcgaccga ctgcgctggc
    14221 gtgatcaact gagcccgctc tccgcatcgc ggctcgtgcg ccgcgctagc acacagctgt
    14281 cgtccagtca tatctcgcgc tgctcgttct attgagcaga actcgagtac agctgtgtgc
    14341 gcgggcgcgc gctgacgcag accgttagct caccgagtgc ccgaccgtca gggtcagagt
    14401 ctcacgggag cggtaccggt gacgcgtaat acttcgtaac cgcgttcttg aactgcccgc
    14461 cgtcggtggt gatataggtc tggttgccgg agcccactcc gaaggtcgcg ccaactccgc
    14521 cggcatcgat gaactcttga acgtgttcga agatgtactt gacgcgatta tcacgatact
    14581 tcccgtccgt tcctccgggc gtgctgctcg gaacgccgaa cggtgtctgc caccaaagga
    14641 tcggtaagcc cgcggcgtcg gtggccttct tgatccaagc taggtactcg tggaagttgg
    14701 gcgaggtctt gttggtttcg tcccaatacc acggcccatc attgcgctgg catgcagggt
    14761 cggtgtgcgc ttcgaagcaa cccgcgtcgc gatcgagaat gtccgtggtg agcaggtcag
    14821 cgtcgctcgc cccgacctcg agcaggaact tcgcgacctt gaccggatcg gggtcggccc
    14881 actgcgatac ctcgaaagca agaaccgcct tgggcgcata cttgcgaccc aacgtaacga
    14941 ggcacttgcc catgcccacg aggttgttcg gcagggccgc gcaatccggg gctagcgcgg
    15001 atacgtttac cttctgattg ctcgggttgc tcttggacgt ttgctgcatg aagccccaga
    15061 agtcgggctc gaactgcacc agagccgggc cattgaagtc accgagtcgc tgaaacagca
    15121 gcttcgctcc atcccagtag gcctgcatgt agctgttgtc ggtcagcgcc gccgcattgt
    15181 tctcgccctg cgcggccatc gaatacaagg tgtacatcgg ggtgaccccc ttggcctttg
    15241 cgctgtcggc gaggatgttc acgaacgtgc cttcggcgtt ccagtctggc cagcccccta
    15301 gccctttgag gccgaccata taggcatagt gcagatccat ggtcacccct agggtatatg
    15361 caccgtcctt gctgtgatca ttgtcgagat cgttccccat cccgatcaag aagttgggtt
    15421 tgcgcccgag gcgcttggcg atatcagccg ccttcttgcc ggctccgctc gccccgcccg
    15481 agctactgcc gccggtgggt gtgcccgccg cgccgccgtt cgaagcacct cccgaagctc
    15541 cccccgtcga agcgccggag ctcgagccgc cggtggcgac ggaaccaccc ttggccgagc
    15601 tgcctgcgct ctgatttccg cccacgcccg gtgctccacc attcccggag cgaccacccg
    15661 tgctgccgcc ggatgcgcca gccttgccgc ccgctgccag cacgccacca gaaccaccta
    15721 ggttgccggg agaaacgccg ccaccggtgc caggctcggc ccctacttcg cccgagcacg
    15781 cttgttgaga tacggcacca aggagtaccg cggcgccagc ggcgaaagac gagaggcgaa
    15841 ctcgtcgagc ggttcgaccg agaaccccct gattgctgtc gattcgatgg ccaacgatct
    15901 gaacacgatt gagcacccga gctcgttcag aagaactgcg cagcttcatg ggaggacagt
    15961 attccgtctg agtgtgctcg cttcaaccgc gtcgtcgtgt gctgactcga gtcgacacgt
    16021 cagctgcgtg acgcgacgaa acccaccctg aatgaattgc ctacgtcgct acgaagtgag
    16081 cgcgcagatc gagtgaaaga atgcgttgtc gaggtcattc gagtcgatcg agcgcgcaca
    16141 cggttaggca cgatttcagg ccatcgaatc acgcgcgcag gcccctcacc tgaactaacc
    16201 gcccgcgccg cagaaggggg cgcgcggctg atcctcgtca agccttaccg cgcccgacat
    16261 cgggctctag agcatgatac cctcgccgaa ctgagcatga ctccgcatga cgaatccaat
    16321 ctgaatcggc cccgcagcgc cgcgagcgcc gccagttccg aagtagcaag cgcggctgcg
    16381 ctcgccaccc ccgccccgcg atcgagcccc gtgcggcgca ctgctctcga gctcgcgatc
    16441 gggctggttg cgggcgtggt gggagcgagc gtgttcggcc cgagcatgat cagcttctgg
    16501 tacgaaccac ccagcaaaga cgccttctcg tgcgcctcta gcgtcgagca agcactgaaa
    16561 gacttcttga agctacagct cggcctcgcg ctcgtcggcg cactcgtgct ggttctagcc
    16621 tcgttcctgg tgcgacgctt cttccggcag agacgcgagc gccgaagcag caataccggt
    16681 gcctgagccg cgttgacacg gcttgcggga cacgccgccg aagaaaacgg ccgcaggaac
    16741 acagccctcc ctcggctctg caccggggac cgcgaggcaa ggccctgcgt ggcagcgcct
    16801 ccgcattgtg gcagtagtag gggccatgcc aactgccagc agtgagcacg agtatccggt
    16861 tctgatcgtg gaacgacact tggacacgtt tgggcacgtg aacaacgcca cctacttgga
    16921 gttgttcgag gaagcgcgct gggacctgat cgtggcgaac ggctacggaa tcgagaagat
    16981 tcgcgagacg cgcaaagggc cgaccatcct ggaggtgcgg ctggagttca agcgtgagct
    17041 gctgaaccga cagcgcatcg tgatccgaac ccgaatcgag tcgtactccg gcaaagtggg
    17101 gacgctcgtt caggtcatgc agaacgatgc gggggacctg ttctgcgtcg ggactttcct
    17161 gatcggcctg ttcgatttgg acgcgcggaa gctgatccta cctacgtcgg agtggaggaa
    17221 agccgtaggg ctcgagctct ccgaacgccc atgacccgcc ctcgaagacc gcctcgactt
    17281 actcgccgtg aagcatgacc gccgagattc caccccgccc cgccacactg accgagaaga
    17341 ttctggcgcg ccacgcgctc gggccactcc cgagcgtgat cacccccgga gcttcgctgc
    17401 gactgcgggt agacttcaca ctagctagcg aactcgcctg gaacggcatg aaccgtacgt
    17461 acgagaggct gggccgaccc gatctctacg atcgagaacg cttctacatc gcgctcgatc
    17521 acacggtaga tccacagagc ttggcccacg accccaagac ccagcgactg gtgcagctag
    17581 ctcgacagct gcgggacgag caccgcctcc cccacttctt cgaacccaac acgaccatcc
    17641 ttcacaccac cttctatcga gacttcgtcc agccgggcca gctcatcttg ggtgctgact
    17701 cgcacacgac cagccacggc gcactcggcg cgttcgcaat cgggctcgga ggcgccgacg
    17761 ttctggccgc catgctgagc ggtgagactt ggctcgaggt gccgcagtgc ttggaagtgc
    17821 gttacgtcgg tagcccgagc ttcggcgtgc tcggaaaaga catgatcttg gccacgctcc
    17881 agcagctcgg ccggaacacg ctcgcgctgg agcgggccgt ggagttcacg ggggacgtga
    17941 tgggcctgtc gctcgacacc cgtttcgcca tctgcaacat gaccgcagag ctcgggggac
    18001 tgtgcgggct gttcgaagcc gatgcgcgag cgcgggcatg gctcgcgaca cgcccgcgct
    18061 cgttcgatga agcggtgtat gaacgagcgg acgctggcgc cgagtacgcc gctcgcgtgc
    18121 gtgtcgattt gaccggcctg ctcccgaagc tcgctgagcc gttttcgccc gacaacgtgg
    18181 tcgacgtggc ggagcgcaca ggcctggctc tggacggctg cttcgtcggc gcctgcacga
    18241 cgaccgaaga ggagctggtg ctcgcggggc tgttgttaga gcgcgcatgg gcctccggta
    18301 tgcgccccga agctcgcgac tcgggccggc gcctactggt acccggcaac cgggagatcg
    18361 catccaagtt gcaggagtcc ggattgctca cgagctacga gcgtgcgggt ttcgtggtgg
    18421 atccgccggg ctgctcgatg tgcctgggcg tcgcgagccg gcgcgccggt gccggtgaaa
    18481 cttggctttc ctcacagaac cggaatttcc cgaaccgaat gggagcgggt tcgctcgctt
    18541 atctcgcgag cgcttgcacg gtcgcggcga gcgcgttcga aatgaagctg agagacccaa
    18601 gccgttggtt cgagcaaatc gaccgtgcgc gcttggcacg aatcctcgcc ggaggccccg
    18661 gaggcgcagc tccccaggtg acctccgcaa aattcgcaac cgagcgtagc ccgctgcagc
    18721 tccagcaggg caaccgagcc gtcgaaagcg ccgattcgag cctaccgcaa gcgacccaag
    18781 gctcggcctc gatttcagaa gtggccgcag acgacagtgc cgcagtgttt ggtaaagcac
    18841 agaggcgcgt gaccacgcgc cttcagggcc gcgtacaacg gttcggggac gccgtcgata
    18901 cggacgccat catcgccggt gagttctgcc atctccaaga gcctgccgaa ctgggtcgcc
    18961 actgcttgaa gtacgttcgc ccggacttcg taacgcgggt tgggcagggc cagaacatcg
    19021 tggttgcggg ccaaggctgg gtctctggca gttcacggga acaggcggtt tgggcactcc
    19081 aaggggcggg tgttcaactc gtaatcgcgc gcagctttgc cttcatccac aaacggaacc
    19141 tggtcaacga agcgttccct ttcctcgagc tcgccgatcc cgccttctac gatctagcgg
    19201 tcgaaggtcg ggaactcagt gtcgatttcg actcgggtga ggtgttggac gtggagtccg
    19261 gccaggtatt ttccgggttg ccgccgcgcg gagcggctcg ccggctgttc gaggctggcg
    19321 gcctaatcgc aaccttcgaa cgcaatgaag gttcatccac gcggtttcaa agcggcttct
    19381 ccaggacctc gaaataaaga tctgcgcgcc ggggcaggcc gaatctcggc tctccatagg
    19441 ggaaagcctc ccgctcgcca gttcttcggt aaccgcagcg ctcgtaatac gcgatcagtt
    19501 ccgagcgctg tgcgattacg gtcattcgca gcttgggtgg gagatattcg cgccgcacga
    19561 tgttctcgac ttccgtgagt aacgcgcgtc ccaaaccatg cccctgaagc cgaggttgca
    19621 ccgcaagcat acctacgtag acgtgctccg ccgcggattt caggcacacg cacgcgacga
    19681 ggtcacccgc tcggcgggcg atcacgacgc gcgacgcggt cgcagcgatc aatgcttcca
    19741 cttcagcgac gtcagtgcgt tgaccatcca gcaaatccgc ttcggtggtc caccctgaac
    19801 ggctcgaatc accgcggtac gcgctctcga ccaaagcgac gatatcttgc acgtcgcccc
    19861 actgcgcacg cgcaaaactc gcccttgcag acaacggagg tatcggccca gacggctcgc
    19921 gattcgacat cagctcacgt ccttctcggt aaaggctctg tactcatagc gcaggccgca
    19981 cttcgggaac gcggccattt tgcaccaaaa gcgcaatacg actgaccgct gttctcactc
    20041 caataccctc atccctgcgt ggcaacggaa accgcgaaag aattgcgttc gtgagctctc
    20101 gagctgacgg gcgaagactt gcgtgccatg actacttcga atgagaagcg ccgcagtgcc
    20161 acggctcaca cgcgcgagaa cgtttacgac gtggcagacc gtacgggttg gcctgagttg
    20221 tatcggactt ctcacggtcc tggcttgttc agtgagccat gaaccagatc cgaacggagc
    20281 gagcggcgcc ggggccgctc ccgcaggcgg aaatacagcc acgagtagct cttcttcgag
    20341 tggtggagtc gtccaccagg gtggtggcac gcagagtggt ggcacgcaga gtggtggcac
    20401 caccggctcg ggcacccaac cgcccccgga tccgctcgcc cttcatcaaa gttgcgcggg
    20461 aaccgctagc ggcggggtgc tcgacagcat cgaggctacg gtccgctgga tcaacgctca
    20521 gccgaagccg ctcacgctcg cgtgttttct gcagagctta cagaagccgc tgaagctcaa
    20581 cgcgacctac agcatcgtga gtgctcagcc gtcgatgggg acccgcagtc cgcggctctt
    20641 catcttcttc gatgggctga ttctgtccgt agtgcccgaa ggcaccggtt cgagtttgct
    20701 cgagctcggt cagctcgacg gcgagacgcg caccgtcaaa gccgaaatcc agttcccagt
    20761 cacgcagacc ctcgctccgg aagcaccgtt cgaacgcgtg ctgtatatgc aggcctttgc
    20821 cactgtttgc gggggctgcc acggcagcga ggcgcgttac gagtccattg cgtacgccaa
    20881 ggcttacgtg tcgaaagcca tccgtccgga gccgtcctac gccgtaccgt tgtcgacttt
    20941 gcagcgcgag ctcgcggttt gcgatgacac cttggagttc gaccgctgcg agatgttgcg
    21001 cgcgctccag caagggggcc ccatttcgca ggattttccg ccgggaatga gcacttcctt
    21061 tttctgactt gcgagcgcga gctgcgctcg tgtcactcca ttccgcattg gccaagacca
    21121 ccgcctcacg ggcggcaccg tgccccaccc cgccgatatc agaaagcccg tccgacggcc
    21181 gttttgcgac cgttcgaacg ggctatgttc gcgagctaga gctcgcctct cggcttcggg
    21241 cgagacgcga aattcaggct tagtagcggt agtgctcggg cttgtacggg ccttctttgg
    21301 gaatgcccaa gtactgcgcc tgacgatcgg tcagctcctc gagctccacg ccaagcttcg
    21361 ccaagtgcaa gcgggcaacc ttctcgtcga gcttcttcgg caaggtgtag acgcggccgc
    21421 gctcgtaacc accgattccg gatagagtgg gtttgccctg tgcggcgagc cacaagtcta
    21481 cctgcgcgat ggtctggttc gtgaaggagt tggacatcac gaagctgggg tggccggtgg
    21541 cgcatcccag gttcacgagc ctgccctcgg cgagcacgat gatggacttc ccgttgggcc
    21601 acaccacctc gtgaacctgg ggtttcacct cgatccagcg atgcttgcgg atgttcgcga
    21661 tctcgatctc cgagtcgaag tgcccgatgt tgcacacgat cgcgcggtgc ttcatcgcgt
    21721 tcatgtgctc ctcggtgatc acgaactcgt tgcccgtcgt ggtcacgaag atatcgccgc
    21781 gtggagcggc cttgttcatg gtggtgacct cgtagccctc catcgccgcc tgcagcgcac
    21841 agatggggtc gatctcggtg atcagcacgc gggcgccctg gcctttcatg gcctgcgcac
    21901 aacccttacc gacgtctcca tagccggcta ccactacgac cttacccgag agcatcacgt
    21961 ccgtcgcgcg catgatgccg tcggggagag agtgccggca gccgtagaga ttgtcgaatt
    22021 tggacttggt aaccgagtcg ttgacgttga tggcgggaaa caacagctcg ttcttctgcg
    22081 ccatctgata caggcgatga acgcccgtgg tcgtctcttc ggaaacaccg cgaatgttct
    22141 tggccgcttg ggcgaagaag ccgggccgtt ccttgatcgc acgcttgacc agcgcataca
    22201 ggatgcgccc ctcttcgtgc ccgggatctt cgttcaagaa cgaggcatcc ttctcggcct
    22261 ttacgccgag atgaacgagc aaagtgagat ctccgccgtc atccaggatc aggttcgggg
    22321 tgccgccatc cgaccattgc agaactcgcc aggagtagtc ccagtactcc tcgagcgtct
    22381 cgccttttac agcgaacact gggacgccgc gatctgcaat tgcggccgcc gcgtggtcct
    22441 gagtgctgaa gatgttgcat gaacaccaac ggatctccgc cccaagcgcc tcgagcgtct
    22501 caatcaacac cgccgtctgg atcgtcatgt gcaacgaacc cgcgatgcgt gcgcccttca
    22561 gcggctgcct ttgcgcaaac tcctcgcgag tagccatcaa acccggcatc tcggtttcgg
    22621 cgaggtcgat ctctttgcgc ccgaaagcag cgagggaaag atcgcgaacc acgtagtcgg
    22681 actttccgtc tttcgctacg ggcgcttttt tataggtgat gatttcggac attcaggtct
    22741 ccttcagaag cactcgggaa tttacgagca atcaacactg cttcgctgcc tcgaaccgag
    22801 attcgcccaa ctagcacgaa ccggcctccc taacgaccaa accctaacga ccaaacccag
    22861 tcggacgcgg aagtttatgc cagtctcgaa gcttggctag agcaaccggg ctagctacgc
    22921 cgcgcgtcgc ccatgcggtt gcgcgaatcc agctgtctgg caggggagca tccgagttcg
    22981 cgcacgccgc accccaaacc acagcatctt cacgacgaat cgagcgcgta ggataggtcg
    23041 ggagagctgg aatcacgcga gatctgagct caaagtgggc agcgccgcag ggtatgggga
    23101 cgcacgcgtg cggcgtcgag tcctagacag gtcgcgggtt ctgtcgccct gcgccttgga
    23161 cgctcgtctg gctcgccggt tcgacccggg agtaggtctc gcgtcacttg cggggcacgc
    23221 cccccagtct cgtcaaggac ccgtgactgc cggcccagcc gcccgtcgtt gctgttcgat
    23281 cgggaaaccc gtagcgcaga atcgggagcg acgctggagg cagagcgttg cagagcagag
    23341 cggtgcaggc agagcgctgc aaagcagggc ggtgcaggca gagcggtgca ggcagagcgc
    23401 tgcaaagcag ggcggtgcag gcagagcgct gcaaagcagg gcggagcagg cagagcgccg
    23461 agagcaagtg ccgcttagcc aatgctgtcc gttgaccagg caactgcgca agacggaatg
    23521 catgcagagc tctcgaatgg aacggggatg gtccatcttt cagaggcgct cgcgaaccac
    23581 cgctccgccg agcgagatac gttcagacga acgcttcata aagaacggcg caagccagtg
    23641 agcgcaatcg caagcagccg acttcatccg cttcgctctc gtcgtacttt gaaacgcggc
    23701 gcgcgaaaga attctttcgg tcctttcgca agaatctttc ggagtctcat gccggatgac
    23761 atccacttga gcaattcctc gattcgcgcg gagcagtccg taaaaatcgc taccttgatc
    23821 aactcacacg agattggccc gggccagcta ccggagaact cgaccgtagc tggttgcagt
    23881 caaaccggca gtcgtccgaa gcccaaactc aaacagtgac tcttgacgca gaacgctcga
    23941 gatagttctg tgtaggttga gggaagcggt ggcgcgggcg tccggcgcac gcgctcccaa
    24001 cacatacccc gcagacgttc tgacaaacgc aaccccaagc acccctcccg cacacgagcg
    24061 caagctcaga cgaacgagcg caagctcaga cgaacgagcg caagctcccg ccatggagtt
    24121 tcgatagatg ggccaaacga tcgagccgac cctcggctac tcagcgagaa acggaaccgc
    24181 ggtgtcgagc accgcgagga tcaacgccag cgatgcgggt cagggccctg cccagaccca
    24241 aatccgcaaa cgacgcgttt cggtaatcat cccgacccgg gaccgcccag actttctcgc
    24301 cgaggccatg acgagcgtgc gtgctctcga gggtcccgat ctcgagttcg agatcttgat
    24361 cggcgacaac ggctcgaatc ccgacacgcc gaaagtcgcc gaacgctttg gagctcggta
    24421 cttcaaggtg gagcgacaag gggccgccgc ggcgcgaaac gcggggatgc gggccgcaac
    24481 gggggactac atcgcgcacc tcgacgacga cgatctctgg acggccgatc acgtgcgccc
    24541 gcacctcgcc ctcatggaag ccaaccccga gatctacacg gtggtaggcc gcatcctgtg
    24601 caccgatacg gagcgcaaac ctatcggtga agcgtggccg cagtcggtcc cggaccacgg
    24661 ggatttgttc atgactttca tgagttactt cccgcaagtg ggaggaacga tctccagggc
    24721 tgcggtgcgg gagaccgtgg gtttgttcga tgaaactctc ctcggcgacg cggactggga
    24781 ttggcagctt cgtatcgcca agaagcaccg catcggcttc gctccggtca cgtgcgtgct
    24841 gttccgacag cgcccatccg gtgaggcgga tgccgtgcat ttgcggcgca ttccgcatac
    24901 ccgccgaatc ttcctgcgtc acgcgctccc cgaatggcgg cgctggaact cgccgacggg
    24961 gctctatcgc tcgtacaaca agtccatggc gccgtatttc atctacttcg taaacactgc
    25021 gcatgcacgc gcggagcggg gagattatcg gggcgccctg gccgccattg ggcacgccgt
    25081 aaagttagtg cccgtgcgtg ggctccgcga gttcgcgcgg agctccgacc tgcgcgggtc
    25141 cctatttcgg gcggtgcttg gcgggcgtct cagtcagaaa actcattagc cgcacgggaa
    25201 gacctgagct aggactccgg tcgggtcatg aagcagacca tcaaaggctt gttgcgccgc
    25261 tctggactgc gacgtagtga tgtggcggcg gcgcgtatgt actgtgagcg ccaggcgctg
    25321 gcgcgttggg ggagaaggcg tccgcgacaa caagggcgca tcctttgcta ccactccgtg
    25381 ggtcagccga gcttcggagt gaacgacatc ccgccccaag cattccggca gcagctggaa
    25441 tgggcgctaa agcaaggcta ccgcttcgta ccggcggaaa agattgcgcg cgacggcgga
    25501 gatccgcgct cgctggccgt cactttcgac gacgccttca agaccgttgg gacccaagcg
    25561 gcccccattc tcgcggagta cggcattccg tggtctgtat tcgtcgtcac caactggtcg
    25621 gacggcggcg gcggctgggc gcccgagacc ttcctgaatt ggtccgagct ccgcgagctt
    25681 gccgataagg gcgcggaaat tgggagtcac tcggtttcac atccggactt cggctacttg
    25741 gacggtgccc acactcgaga cgagctgcgc cgctctcgcg agatcatgga ggagcgcttg
    25801 gggttcgcac cgcacagctt cgcgatccct ttcggtcaat ccaagaactg gtcgcctgtt
    25861 gccactgaag cggcgcgcga agcaggatat cgaatcgtgt acgcgcaggc ggagggctcg
    25921 cggccgatgt ttacggttcc gcggacattc atcactcact acgataatgg ccgtgtgttc
    25981 gaggccgccc ttcagggcgt gttcgatcac tgggaagagt gggtctgaga gcggttgctc
    26041 cggagcgctg atcggctccg cacgcgtagc cgtcgtcgtg cttcgctcgg gcaaggcggc
    26101 ccgagcgaag acttcgccga agcccgcgcg gctacatgag accgcgcggc tagacgagac
    26161 tacgagcggg tcagattgcg ctgataaatc tcttccagac gccgcgccgt agttgccgcc
    26221 gagaactcct cctcgacatg cttgcgcgcg gcttgaccga gctgttcttt gaaggacttg
    26281 gattcgagga ttcggattac ggactccgcg atggagctcg agttgcgtgg ctcggcgcac
    26341 agcccggtct gctcgtggat caccgcttct cccttgcccc cgaacgtgat caccacggcc
    26401 cgactcatgg ccattgcttc gagtagcgca atcgacatcg agccgattgg caccggcaat
    26461 acgaatgcat ccatcaaacc gaagaatgga cgcggctcgg tctgaaatcc ggtgaagcga
    26521 gtgaactctt tgaggcccac ccgttccact tgcgcctcta gctcaccacg caagctgcca
    26581 tctccaacca tgagcagata ggctttcggc cgcgccgcgc gaatcttgac catggcgtcc
    26641 accaggtact cgagcccctt gttggactcg aagcgcacca tggtgccgac tacctctgcg
    26701 tcctcgggca gagagtagcg cgcccgtagc tccgccaaag taggctcggg aattggagtg
    26761 aagtcgtcca ggtcgacgcc atttttgacg atgaaggtac ggtcgggcgg agtgtacaaa
    26821 aactccttgc gcgccaagta gttcttctcg gacacgcaga tgatgccgga cgcaatccga
    26881 gccgtgaagt ttcgcaacag ctcgtggtgg cgcatctccc gctgctcggg ggcgaggtgc
    26941 tcggtgatgt aaaccttgcg cacgcccgcc gcgaacgcgg ccaagtacga gaggttctgg
    27001 ccacgatagt agccgttgtg catgtgcatc acgtcgatct tccagtcgcg gatctgctgc
    27061 atcagcacgg gccaattctg cagatgaaaa cgatcgaacg tgatggcggc gaagtgatcg
    27121 ctgtagcgtt gcaggttttc tgcgaacggc ttgacggcgt ccaacgcggg acagatcgag
    27181 aacacctcga agcggcgccg gtccaggtga cgcgcgatga gctcgatgtg ctcctccatg
    27241 ccgccgacga agcgtgtatt agtatagagt aacacgcgga cccttcgccc caacggcaac
    27301 tccggagcaa cgaagcggcc agagcgatcc ggatctcgcg aagaagacgg ctcagccgat
    27361 tccggctcga cgggccgccc cgacccggtg accctggatt gaggcgcggt agaggcttcc
    27421 gagttcccta atgatgcggt ggcggtttcc atacttccag gctccgtgtg taactcgcga
    27481 gaatagatcc ggcacaagcc gtgaggcaag gtaacttagt cgcctacatc ggagctttcg
    27541 atatcagtcg atatcaaagc tcgagcgcgc actcgtggag ggcctgagct gtgtggcgcg
    27601 agacgcggct cttgggctta gaaagcgccg gtttcttccg gctcccgctt cgatttgccc
    27661 gggctgttca gctgttctga tgtgttctg
//
LOCUS       DASTKU010000285         3976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1840451, whole genome shotgun sequence.
ACCESSION   DASTKU010000285 DASTKU010000000
VERSION     DASTKU010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840451"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..409
                     /locus_tag="VFQ61_10330"
                     /pseudo
     CDS             279..409
                     /locus_tag="VFQ61_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612006.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1187..3364
                     /locus_tag="VFQ61_10335"
     CDS             1187..3364
                     /locus_tag="VFQ61_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C, phosphocholine-specific"
                     /protein_id="HET9954893.1"
                     /translation="MSSGNRREFLRGFAALSAAGATLTFPPEIQRALAISPHRRTGTI
                     QDVEHVVILMQENRSFDHYFGTLAGVRGFGDRFPIPVPNVAGRRRKSVWQQPNAKADQ
                     SPAWLVPFPLNTQQKFEHMRVTGTPHSWRDAQYAWADGKLEAWPRFKNNHSLGYFTRE
                     DLPFQFALAEAFTLCDAYHCSFQGGTNPNRLFLWSGTNDGRAAGNGPAIDNSYDNLEH
                     DPSGGYTWLTYSERLQSAGVSWQVYQNMADNFTDNPLAGFRRFRDAYAGAAGALAVLK
                     QRGLSTRDLELLKQDVLNGSLPQVSWIVATAEGSEHPGPSSPAQGAEYTARVLDALTA
                     NPEVWSKTVLFVNFDENDGFFDHVPPPAPPSLVRAMSARADAADEFAPAGGSTADTTG
                     EYHEFLGLDEDAALENKPYGLGPRVPMYVVSPWSRGGWVNSEVFDHTSVIRFIEARFG
                     VREPNISAWRRTVCGDLTSAFDFGRSDPQSFVDELPDTVDSAARARALPSTTTPAAPE
                     TIQLPLQDYGVRLSRALPYELNVHARVDVRERSVELTFSNTGHVGAVFHVYDRRRLDR
                     GPSRYTVEPGKQLRDTWLADERSADYDLWVLGPNGFHRHFTGNFWSMLRPYRNPEVRI
                     VYESARGGLGIQLRNPSQAACSAIVESNAYFFAERTLLALPAGHSVHHEWSLRRSANW
                     YDFTLRIQELPGYARRFAGRVETGRDSFSDPAMGGQALIDWTG"
     gene            complement(3327..3902)
                     /locus_tag="VFQ61_10340"
     CDS             complement(3327..3902)
                     /locus_tag="VFQ61_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET9954894.1"
                     /translation="MATWLWTWLAPVEPVLRLLIATLLGALVGWDRQHQNKPAGTRTH
                     MLISLGAAAFTLGPFSVAAMDLESTSRVIQGVAGGMGLLAAGSIIQSKDKVSGITTAV
                     SVWACGGIGAACGLGNYLVATSAAVIATLVLLIGRMHDMWERSRIKPAASSSLVSTDR
                     ARSGHTADAAPGYPTQAHSLSPSNQSAPDHP"
BASE COUNT          711 a         1224 c         1316 g          725 t
ORIGIN      
        1 ccgcccattc aactgagcgc gccgttcagc gcgggggcgt gccagcacag gcgtggtgca
       61 ctcggcactc tcgataacgc gctggccctc gatgtcgccg tgtcgcctgt gcgctcgcgc
      121 cgttcaggct cgtccctcac atttagatca cttcgtcgac gggcgcgttt acctgctggc
      181 accgttctga tctcggtgtc cgcagaagag aaggccgggt ggagtcgtcg caaaggtcgt
      241 ttcaagcaaa cacggactca aagcataacg ggcccgtcgc tcccaacctg cttgcgcgcg
      301 acttctccga aaccgagccc aatcgcgcca ggtgaccgac gtcgcggccg tcgcgacggg
      361 cgagggctgg ctcaatctgg ccgtggtgct cgacttgttc tcgcgccgcg ccgcgccgcg
      421 ccgcgtcgat ggcggatggc ggggacacca gctcgaacag agaccctgct agcgcccgaa
      481 gcgttgagcc aaactccgcg ctcacccgcg gagaagcccg agcgggaagg gtgattgttc
      541 ggaacgccct tgccagaagc ttcttcgcga ctctccggag agcgcaccct gtccacgtgt
      601 tcgcggagac ttcatgcgag caatacctgg cgactggctg cgcgcatacg tgggcgaagg
      661 ccccggcgac cggcgacgac tgctcgacgt tagacctcga gctgtacggc gtggcattca
      721 acccctgagt cgtcttccgc ggtgaggagg cgcaacttcg tatcctggcg cggccgctga
      781 agatggcccc ggatgacctc gaatgggttc acctgtgagg ctctgaattg ttggccgctt
      841 cgtgaacggg cgcgccgttc ggtgaggttg cggtgtttgc gcagacgaga cgcccgcggt
      901 gctttccaat accgcgcagg cccctcgcag tcgttactca ttgttcctgc gcgcccacgg
      961 gttccacgag tgtctccgtg cttcaccccg gtcagacgtg gcgttgttac gggggccggc
     1021 gcggtagggt ggggatttgg gacgaactcg gtctgcgctc gggagcacga gggtcgggca
     1081 gcgcgcacgg aaacagagtg gccgacggat tgttggcgag cgagagaggg tgaattggtt
     1141 cggggttgtc acgagcgcgc caaaggggga gcggcagatt gcaaacatga gttctggaaa
     1201 ccgcagagag ttcctgcgcg gctttgctgc gctctcggcc gccggagcga cgctgacttt
     1261 tccaccggag attcagcgcg cactggcgat ttcaccgcac cggcgcacgg gcacgattca
     1321 agacgtggag cacgtggtga ttctgatgca ggagaaccgc tcgttcgatc actacttcgg
     1381 gacgttggca ggggtacggg gtttcgggga tcgtttcccg atcccggtgc caaatgtggc
     1441 aggccgcagg cgcaagagcg tatggcagca accgaacgcc aaggcggacc agagcccggc
     1501 gtggctcgtg ccttttcctc tgaacacgca gcagaagttc gaacacatgc gcgtcacggg
     1561 cacgcctcac agctggagag acgcacagta tgcttgggcc gacggcaagc tcgaggcttg
     1621 gccgcgattc aagaacaatc actctctggg ctacttcacg cgggaagacc tgccgttcca
     1681 gttcgcgctg gcagaggcat ttacgctgtg cgatgcctat cactgctcgt tccaaggcgg
     1741 cacgaacccg aatcggctat ttctttggag cggcacgaac gacgggcgcg ccgccgggaa
     1801 cggccccgct atcgataact cgtacgacaa cttggagcac gatccgagcg gcggctacac
     1861 ctggttgacg tatagcgaac gactgcagag cgcgggcgtg agctggcaag tctaccaaaa
     1921 tatggcggac aacttcaccg ataacccgct ggctggtttt cggcgcttcc gcgatgctta
     1981 cgcgggcgcg gccggagcgc tcgccgtgct gaagcagcgc gggctcagca ctcgagatct
     2041 ggagctgctg aagcaagacg tgttgaacgg ttcgctgccg caggtgtcct ggatcgtcgc
     2101 gacggcggag ggctcggagc accctgggcc gtcgagccct gcccaaggcg cggagtatac
     2161 ggcgcgcgtg ctcgatgcgc tgacggccaa tccggaggtg tggagtaaga cggtgttgtt
     2221 cgtcaacttc gacgagaatg atggcttttt cgatcacgtg ccgccgccgg ctccaccgtc
     2281 gctcgtccgc gcgatgtcag ctcgcgctga cgccgccgat gaattcgccc ccgcgggcgg
     2341 ctcgaccgcc gataccaccg gtgaatatca cgagtttctg ggcctcgacg aagacgctgc
     2401 cctcgaaaac aagccgtatg ggctgggccc gcgggtgccc atgtacgttg tttctccgtg
     2461 gagccgcggt ggttgggtga actcggaggt cttcgaccac acgtcggtga ttcgcttcat
     2521 cgaggcgcgg ttcggggtcc gcgaaccgaa catctccgct tggcgtcgta cggtctgcgg
     2581 agatctcact tcggcgttcg acttcgggcg gtcggatcct caatcgttcg tcgacgagct
     2641 gccggatacg gtcgattcgg cggcccgggc ccgcgcgttg ccgagcacca ccacgccggc
     2701 agcgcccgaa acaatacagc tcccacttca ggactatggg gtgaggctat ctcgcgcgtt
     2761 gccgtatgag ctcaacgtgc acgctcgcgt ggacgtgcgg gagcgcagcg tggaactcac
     2821 gttctcgaac acggggcacg tcggcgccgt attccatgtc tatgaccgcc ggcgtctgga
     2881 ccgaggccca agccgctaca ccgtagagcc gggcaaacaa ctccgcgaca cttggctcgc
     2941 agatgaaaga agcgccgatt acgatttatg ggtgctagga ccgaatggtt ttcatcggca
     3001 tttcaccggc aacttctggt cgatgctgcg gccgtaccga aatccggagg ttcggattgt
     3061 gtatgagtcc gcgcggggtg ggctggggat tcagctccgg aacccaagcc aagcggcgtg
     3121 cagcgcgatc gtggagtcga acgcctattt tttcgcggag cgcacactgc tcgctttgcc
     3181 agcgggccac tccgtacatc atgaatggtc gctgcgccgg agcgccaact ggtacgattt
     3241 cacgctcagg attcaggagc ttcccggcta tgcgcggcgt ttcgcgggtc gtgtggagac
     3301 cgggcgggac tcattcagcg acccggctat gggtggtcag gcgctgattg attggacggg
     3361 ctgagggagt gcgcctgcgt cgggtagccc ggggcggcat ccgcggtgtg tccgcttcgg
     3421 gcgcgatccg tggagaccaa agacgacgag gccgcgggct tgatccgtga tcgttcccac
     3481 atgtcgtgca tgcggccgat gagaagaacg agagtggcaa tcacggccgc cgaggtcgca
     3541 accaggtaat tgccgaggcc gcatgccgcc ccgatgccgc cgcaagccca gacgctcacc
     3601 gccgtggtga tcccggagac cttatccttg ctctgaatga tgctgccggc cgccaggagc
     3661 cccatgccgc ccgctacacc ttgaatcaca cgcgaagtgg actcgagatc catggccgcc
     3721 accgagaagg gacccagggt gaacgcggcc gcacccagcg atatcaacat atgggttcgg
     3781 gtgccggcag gtttgttttg atgctggcga tcccagccca ccaacgcgcc caacaacgta
     3841 gcgatcagca agcgcaacac gggttcgaca ggagcgagcc aagtccaaag ccaggttgcc
     3901 acgcggcgga agtgagcaag gcgcatgccc tagacaggcg aaggctgcga ttcagccgag
     3961 cagcctttct cgtcct
//
LOCUS       DASTKU010000286        11975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1171256, whole genome shotgun sequence.
ACCESSION   DASTKU010000286 DASTKU010000000
VERSION     DASTKU010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11975
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171256"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VFQ61_10345"
     CDS             <1..816
                     /locus_tag="VFQ61_10345"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET9954895.1"
                     /translation="PIRPWNNVPVLSYLFLRGRASCCQVPISPRYPLVELSGGLLAWA
                     ILRCIVFELPVGASLGQALGLFGLYLALGLALLALAFIDLEYMLLPDSLTLGGALLGL
                     LSAGFRGPGYTASLLGALLGFLLIYLPFDLLHRLLRGYPGMGLGDAKLTLLAGAWFGA
                     RGALFALFAGAVQATLVVLAVFLARGKIEEPEAVRLERQELEAAIENATGEERAELER
                     ERELDPAMRAPETGLHRARVPFGPFLALATLEYALLERAGLSSLFDVFTDAWS"
     gene            807..1241
                     /locus_tag="VFQ61_10350"
     CDS             807..1241
                     /locus_tag="VFQ61_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954896.1"
                     /translation="MVLRTGVVTLRRVVLLIACLEMGGLGACKRASPPTRPVAEEFIE
                     VPYPPPPAQIEEIQPELAGRPECRYVDGHYEWRSRRWQWVTGDWFVTPRDCRHAAGVL
                     SYGRPPNPRLYYTPPRWYPTNGNAACEPAVPCLNRLPQNNSP"
     gene            1205..2128
                     /gene="prmC"
                     /locus_tag="VFQ61_10355"
     CDS             1205..2128
                     /gene="prmC"
                     /locus_tag="VFQ61_10355"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET9954897.1"
                     /translation="MPEPPSPEQQPLSSPEPWSIARVLRFATEDFEKRRFSSPRLDAE
                     LLLGHVLGVDRVRLILDATRPLTEPELQAYRELIKRRRRGEPVAYLRGQREFYGRVFR
                     VDRRVLVPRPETELLVEVALTRTRARSLYGRALDLCTGSGCVATTFARERRTWRVTGA
                     DISEPALQVARDNALRLGAIHNLDFVQGDLFEGVPPKARFELVTANAPYIPHQDIAGL
                     DSDVRDFEPKLALDGGADGLVIIRRIVAQASARLVPGGVLALEVGYDQAPRVSELLHA
                     AGFDAVQIQKDYAGHQRIVSAKSSSTGNSTP"
     gene            complement(2159..2800)
                     /locus_tag="VFQ61_10360"
     CDS             complement(2159..2800)
                     /locus_tag="VFQ61_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954898.1"
                     /translation="MRLLSVWLGLATSAVVFLPAVSAFAAEDDWDGGYETKAERRSGF
                     TASVGLGFGLGSAVGYPNEISKFDDPAYESNTGAALGMQTSIWAGGALRDWFNFGLGL
                     NSLSATKGALKARGSSFIAHIEAFPLWSLGGKARDLSFFTEIGAGSMNIEGGPEKAEG
                     GLMSTLTLGSSFELLRLGSFALGPTVAGTYMYSQSLEAYGVMAGIRASFYGGP"
     gene            3193..4191
                     /locus_tag="VFQ61_10365"
     CDS             3193..4191
                     /locus_tag="VFQ61_10365"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HET9954899.1"
                     /translation="MELYTLKNRNGLTVQVTNYGATVTSVLVPDRNGTFDDIVLGFDH
                     LDAYLTNGAFLGATVGRVANRIRNAKFELDEKTYSLAANDPPHHLHGGKQGFDKVLWS
                     AAPFDSPEGPGLRLTYAAKDGEEGYPGNVQVSTIYTLSHDDELRVEMEASTDQTTLVN
                     LAHHTYWNLAGKAAPPVLDHELTLFASNFTPGDPVVPTGVVEPVQGTPFDFTRGKPIG
                     KDLKAVGGNPVGYDHNFVVDGDPHTLRPVARVREPRSGRVMTLDANQPGVQFYSGNFL
                     DGSLKGKAAHYQQYTGFCLETQKFPNSVNVPAWREEAILRPGQVYKHLMIHRFRSE"
     gene            4223..4612
                     /locus_tag="VFQ61_10370"
     CDS             4223..4612
                     /locus_tag="VFQ61_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9954900.1"
                     /translation="MPTVLCDSSTTKAMAFIATERPERALAFYRDVLGLRLLEDTPFA
                     LVFDAFGTMLRVQKAHSVVLAPYTSFGFEVKDIRATAQALAMEGVKGVRYSHFDQDEL
                     GIWLAPGGARVFWFKDPDGNLLSLTQF"
     gene            complement(4678..5001)
                     /locus_tag="VFQ61_10375"
     CDS             complement(4678..5001)
                     /locus_tag="VFQ61_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9954901.1"
                     /translation="MTFGREVRRRRAALGLTLEQLAERAGLTPNYIGTVENGRRDPSL
                     STVLALARGLGAAPGELLGGVGELSPTSTEAATLIETLLPDVQVAILELLRMLSRKRR
                     VTREP"
     gene            5589..7364
                     /locus_tag="VFQ61_10380"
     CDS             5589..7364
                     /locus_tag="VFQ61_10380"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9954902.1"
                     /translation="MNANRSHAVSQTPILLSRGKRALLSATACACTTLALIGCNRSNS
                     SSLSKLADDNNVPEFPAPAENGPKLGCVANATPIFEKATTHSKVIGFLHAGASVARSE
                     APVRKARDCESGYFAIFPRGFVCLNQGATLDMAHPTLAAMAIQPALDRSLPYTYGRAR
                     ASSALLERDARQSDAVVEVQKFAQGSGAAVVGSWTARELSRETERERVESQKSGDSRK
                     DSDSAAVLAKEVLAKEVPAREQDSSPPQRLALLTSGRFARARDLEAARPSSFAGQSLA
                     ENGAALPVAFVIKRGVFKYRVSGDVLEKAEQLEFHDKFGLTSRYRTQDGNRFWLVQGE
                     PERWVRERDVTRASARLKLPEFVSAPGANDAQRWIDISVGSGTLVAYVGKRPVYTTLV
                     SVGRDRLAEYVTDGAAVTRQGTFEVVAKHVSWLDLGAIGVAAMAANPNDAASKANADA
                     GGTVRNAAFASDTNNAAAPATDSGPTTAGLNPVASIQLFSRLSDRSSKLAFYDVPWLV
                     ELSSGQFLHGAYWHDRFGIEHGPGDVALSPTDARWLFEFVTPTLPKGWHAVGNGSADK
                     VAAGGKAVGVDDGGSVEKTLVVIHK"
     gene            complement(7398..8261)
                     /gene="metF"
                     /locus_tag="VFQ61_10385"
     CDS             complement(7398..8261)
                     /gene="metF"
                     /locus_tag="VFQ61_10385"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET9954903.1"
                     /translation="MKISDKLASSARPAFSFEFFPPKDAQGVERLFETAGKLARYSPA
                     FVSVTYGAFGSTRGLTVDLVKRIKRELGLETMAHLTCVGAERHEIAEVLDQLARGEID
                     NVIALRGDPPQGSTTFTPAANGFSHASELVQFIKSRWSFCVAAACYPEKHPEAASLER
                     DLDWVKHKTELGAELLLTQLFFDPNVYFRFVERARERGVRVPIVPGVMPITNLSQVKR
                     FTAMCGASIPSELLGRLEASGGDTEAVQRIGVEYAVAQCRRLLEGGAPGIHFYTLNRS
                     RATMDVLEQIA"
     gene            8352..9827
                     /locus_tag="VFQ61_10390"
     CDS             8352..9827
                     /locus_tag="VFQ61_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET9954904.1"
                     /translation="MHIHIVAVSGTGMGSLAGLLVELGHEVSGSDIAFDPPMGPALRE
                     YGVRCLTGFDARHLEPAPDLVVIGNVCRPDNPEARAALERGIPYTHIAGALSRFALEN
                     TSPLVVAGTHGKTTTTALSAFLLDKTGFAPGFLIGGLPKDFARSFRAKGPRRLGQLPA
                     AEGHAAHRRKTPFVIEGDEYDTAFFEKTAKFLHYRPEVAILTSIEHDHVDIYPTWSAY
                     VEAFRRFVALLPEEGLLIANAADPVVRELAREARAEVAYFALDGEDTGGTAPHWLASP
                     AQVGPTGTSFDLFAGGVSCGRLALSVPGRHNLKNALAALAAAAQGYGAELREIGPLLA
                     QFQGVRRRQDLLGTPRGIFVYDDFAHHPSAVRETLLALRQRHSTGKLIAVFEPRSATA
                     CRKLHQDDYPAAFGPADFVLLAPLGRSQLPPGEALDLSVLVSDLRKLGKDAELAPDLS
                     GIPQRIAELAAPGDVVLLLSNGSFGKIPERLPGVLAGLSAT"
     gene            9846..10346
                     /locus_tag="VFQ61_10395"
     CDS             9846..10346
                     /locus_tag="VFQ61_10395"
                     /inference="COORDINATES: protein motif:HMM:NF002486.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HET9954905.1"
                     /translation="MNRLTCPCGKGESLETCCGPFLNGSKIPQTAEELMRSRYTAFAV
                     GNVDYIVDTHDPDRRGEVDRNNTEAWSKQSEWLGFELVKSELGGPSDDAGVVEFVAKY
                     RAKGVTIAHRERSVFRKQNGRWWFVDGQIVNGPPVRREGPRVGRNDPCPCGSGKKYKK
                     CHGAEA"
     gene            10402..>11975
                     /locus_tag="VFQ61_10400"
     CDS             10402..>11975
                     /locus_tag="VFQ61_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954906.1"
                     /translation="MPEPSRVQADEPSAIERPTLLVIGRSDSFQEALVERLSQRALNV
                     ETALPERAVEATVVTAPDLIVLGPGLGEKGLLEQLVSSAVSSVIPIVLLQDEEPLDAR
                     LRAFRRGAAAVVARTASVDALAERLATLAREIPERDGDVGSEVVSALAGSAGEATLEE
                     FVRMLASELRSGILSVESGVVNAPVRLVLGAGRPLLSAVDEFVARISQHVLHAEPLRY
                     EFDDRAGGTLAVWDELPSRGRLGRGDVSGLRLMLCDDDPARIDQVAQQLRERGAHVAV
                     CSLSPDALELADLRQFDPEVVVMSEVAIAGAGYALLRRMKQDLRLRWASLLVVRWEQI
                     FELDRTVPGFDRLTAMLADLAEVDRGMSDRAELGDAFDARLEVSGPARCLRALARTGR
                     ALSLTIQHARAELRVELSRGLVVSAWARSRQDSSTWEGALALSVMLGLRTGRVHVTPI
                     EQPSAANLMAEVDLALDWAERETSPLAPSRPSSDALSVAGARAAVVVSPTLAPALDPA
                     TQSAFEVASATSWPVA"
BASE COUNT         2051 a         3671 c         4000 g         2253 t
ORIGIN      
        1 ccaatccggc cttggaacaa cgtgccggta ctgagttact tgtttctgcg cgggcgggcc
       61 agctgttgtc aggttcccat ctcgccgcgc tatccgttgg tcgagctgag cggcgggctc
      121 ttggcttggg cgattctgcg ctgcatcgta ttcgagctgc cggtcggcgc tagtttaggg
      181 caggctctcg gcttatttgg gttgtatctc gcgctcgggc tagcgctgct cgcgctcgcg
      241 ttcatcgatc tcgagtacat gttgttgccg gattcgctga ccttgggcgg cgcgctgctg
      301 ggcctgttgt cggcaggttt tcgcggaccg ggctacacgg cgtcgctgct cggtgcactg
      361 ctcggttttt tgctgattta cctgccgttc gatttgttgc accgcttgct ccggggttat
      421 cccggtatgg gcttgggtga cgccaagctg acgctcctcg cgggcgcttg gttcggcgcg
      481 cgtggcgcgc tgttcgcgct gttcgcgggc gccgttcagg ccaccctggt ggtgttggcg
      541 gtgttcctgg cgcgtggaaa aatcgaggag cccgaggcgg tgcgcttgga gcggcaggaa
      601 ctggaggcgg caatcgagaa tgcgacgggg gaagaacgag ccgagctcga gcgtgaacgc
      661 gaactcgacc cggcgatgcg tgcgcccgag acggggttgc accgcgcgcg ggtgccgttt
      721 gggccgttct tggcgctcgc gactttggag tatgcgctgc tggaaagagc ggggctgtcc
      781 agcttgttcg acgtgtttac ggacgcatgg tcttgagaac gggtgtagtt accttgcgcc
      841 gtgtcgtgct gctgattgcc tgtctcgaga tgggaggatt gggcgcgtgc aagcgagcct
      901 cgccgccgac gcgcccggtc gcggaggagt tcatcgaagt gccgtatcca ccgccccccg
      961 cgcaaatcga ggagattcag cccgagcttg ccgggcgacc ggagtgccgg tacgtggacg
     1021 ggcactacga gtggcgaagc cgccgctggc agtgggtcac gggcgattgg ttcgtcactc
     1081 cacgggactg ccgccatgcg gcgggcgtgc tttcatatgg tcgcccgccc aacccgcgcc
     1141 tttactacac gccgccccgc tggtacccga cgaatggtaa tgccgcctgt gagcccgccg
     1201 tcccgtgcct gaaccgcctt ccccagaaca acagcccctg agctctccgg agccgtggtc
     1261 gatcgcgcgg gtgctgcgct tcgcgactga ggacttcgag aagcgacggt tttcgagccc
     1321 gcgtttggac gcggagttgc tgctcggtca cgtgctcggg gtggatcggg tgcggttgat
     1381 cctggatgcg acgcgaccgc tcaccgagcc cgagctgcag gcttatcgag agctgatcaa
     1441 gcgacgtcga cggggggaac cggtggccta cttgcgcggt caacgcgagt tctatggccg
     1501 tgtgttccgc gtcgatcgtc gcgtgctcgt gccgcggccg gagaccgagt tgttggtgga
     1561 agtggcgctg acccgaaccc gcgcgcgttc gctgtacggt cgcgcactcg atctgtgtac
     1621 tggctctggt tgtgtggcca cgaccttcgc gcgcgagcgc cggacgtggc gggtcacagg
     1681 cgcggatatc tccgagcctg ccctgcaggt agcgcgggac aacgcccttc gactcggcgc
     1741 cattcacaac ctcgatttcg tgcaaggcga tctgttcgag ggggtgccgc ccaaggcacg
     1801 cttcgagctg gtgaccgcga atgcgcctta cattccgcac caggatatcg ctgggctaga
     1861 cagcgatgtc cgcgatttcg agcccaagct cgcgctggac gggggcgccg acggtttggt
     1921 cattatccgc cgcatcgtgg cccaagcttc ggcgcgtcta gttccaggag gagtgctcgc
     1981 gctcgaggtg gggtacgacc aagccccgcg agtctctgaa ttgctacacg ccgcggggtt
     2041 cgatgcggtg cagatccaga aagattatgc gggccaccag cgcatcgtca gcgccaagag
     2101 cagcagcact gggaacagca cgccctaacc gatctcgcgc ccgagcgagc ctgcagcgct
     2161 acggcccgcc gtagaagctg gcgcgaattc cggccattac gccgtaagcc tcgagcgact
     2221 gcgagtacat ataagttccc gctacggtgg ggccgagcgc gaacgagccg agccgcagca
     2281 gctcgaagct gctgcccaaa gtcaacgtgc tcatcaggcc gccttcggcc ttttccgggc
     2341 caccctcgat gttcatgctg cccgcgccga tctcggtgaa gaagctcaag tcacgggcct
     2401 tgcccccgag cgaccagagc ggaaacgcct cgatatgcgc gatgaaggag ctgcctcgcg
     2461 ccttcagcgc acctttggtg gcggagaggc tgtttaggcc caagccgaag ttgaaccaat
     2521 cgcgcagggc gccgccggcc caaatagaag tctgcatgcc gagcgcggcg ccggtattcg
     2581 actcgtacgc gggatcgtcg aacttggaga tctcgttcgg atacccgaca gcgctgccaa
     2641 ggccaaatcc caagcccacc gaggccgtga agcccgagcg tcgctcggcc ttggtctcgt
     2701 agcctccgtc ccagtcatcc tccgccgcaa aggcggaaac ggccgggaga aatacgacgg
     2761 ccgaggtggc caagcccaac cagactgaga ggagcctcat ggccccgagt ttgtcggctg
     2821 gttgccgagt tccgtcaagc tcctccccat tcaaaaaagt ggcttcagag aagccggctg
     2881 gaaagatggg cggtgcgccg gccgaagatc cgaggcccgc cgcgagctca gaaggctcta
     2941 gccagcagcc tgctcgaggg ctttcgggcg gccgtcggtc gaagtgacgc ttgccgtcgg
     3001 ggcgagcctt cggtccgctg cgcgagggcc attcgcggct ctgctgttac aagccagctc
     3061 cgctggtacc gatgcgacgg ccgcgctcga cccagcgggc aacccgctct cgagcgagcc
     3121 tcaacgaacg tcagttggcc cggaatcagc gcggcccagg cgacgtcatt tgatacactg
     3181 cgtggcaccg ccgtggaact ctacacgctc aagaaccgaa acgggctcac tgttcaggtc
     3241 accaactacg gggccaccgt cacttcggtg ttggtgcccg atcggaacgg cacgttcgac
     3301 gatatcgtgc tcgggttcga tcacctcgac gcgtatctca ccaatggcgc tttcctcggg
     3361 gcaaccgtgg gccgcgtcgc gaaccgaatc cgcaacgcga agttcgagct cgacgaaaag
     3421 acctattcgc tggccgcaaa cgatccccca caccacttgc acggggggaa gcaaggcttc
     3481 gataaagtgc tgtggagcgc cgcgccgttc gactccccag aggggccagg gctgcgcttg
     3541 acctacgcgg cgaaggacgg cgaagagggc taccccggca atgtgcaggt atcgaccatc
     3601 tacacgttgt cgcacgacga cgagcttcgg gtggagatgg aggcgagcac ggatcagacg
     3661 accctcgtca atctcgctca tcacacttat tggaacttgg cgggcaaagc ggcgccgccg
     3721 gtgctcgacc atgaactcac gttgttcgcg tcgaacttca ccccgggcga tccggtggtc
     3781 ccgacgggcg tggtggagcc cgtacaaggc acaccgttcg atttcacgcg cggtaagccg
     3841 attggcaaag atttgaaggc ggtgggtggc aatcccgttg gttacgatca caacttcgtc
     3901 gtcgatggtg acccccacac gttgcgaccc gtcgcacggg tgcgtgagcc gcgctccggg
     3961 cgagtcatga cactagacgc caatcaacct ggcgtgcagt tctattcggg taacttcctg
     4021 gacggcagct tgaaaggcaa agccgcccac tatcagcaat ataccgggtt ctgtttggag
     4081 acccagaagt tcccgaactc agtcaacgtg cccgcctggc gagaagaggc tattctgcgt
     4141 ccaggccaag tctacaaaca tctaatgatc catcgcttcc gctcggagta actgtggagt
     4201 gagggatcgt cagggggaaa tcatgccgac ggttctctgc gattcatcaa cgaccaaggc
     4261 gatggcgttc attgctacgg agcgccccga gcgagcactc gcattctatc gcgacgtgct
     4321 cgggcttcgt ctgctcgaag atacgccgtt cgcgctcgtc ttcgacgcct tcggcacgat
     4381 gctgcgcgtg cagaaggcac actcggtggt actcgcgccg tacacgagct tcggcttcga
     4441 ggtgaaagat attcgtgcca ctgcgcaggc gctggccatg gagggcgtga agggcgtccg
     4501 ctactcacat ttcgatcaag acgagctcgg gatctggttg gcccccggcg gggcgcgcgt
     4561 gttttggttc aaggaccccg acggcaacct gctctcactg acgcagttct gatcggcgca
     4621 gttcagtcct ggttcttgcc gcgagatcga tagcgcggcc ccgttgtcag cgcgatctca
     4681 aggctctcgc gtcacccggc gcttgcgcga taacatccgg aggagctcga ggatcgcgac
     4741 ctgcacgtct ggaagcagag tctcgatcag agtcgctgct tccgtcgagg tggggctaag
     4801 ttccccaact cctcccagta actcgcccgg tgctgcgcca aggccacgcg ccaatgccaa
     4861 gaccgtgctc agagagggat cacggcggcc gttctcgacg gtaccgatat aattgggggt
     4921 taagccggcg cgttcagcga gctgctcgag ggttaaaccg agcgctgcgc gacgccgacg
     4981 aacttcacgt ccgaacgtca ctggaaccaa ggttctcttc gttcctgtgc ctgacatgaa
     5041 ccactgatag tgttgacctt caacacgatc gacagaagaa acgcgggtca caaccgaccg
     5101 acgtaccaaa atggccgtcg ggcactcgtt caggcgatgc ttcgttcgag gttgccaagc
     5161 acgttggaaa ctgcggtttt tgtgccttag ggtacgagat gaatcgttca cgccacgcgc
     5221 tggcccccct tcgacgatga tcgatcccgg gctcgcgccc ggcttctagc cagccgaatc
     5281 gcactcgaga ttcgagccga atctacgcag ccagctcgag tcagtagcca gttctggcga
     5341 gggcagtggc gcaccaatgc gcgcacaaaa gttggccacg agttgcgcct tcaggcaagc
     5401 actctgcggt gaaatcccgt ttcgcacgag cccaggtgac gcgctcggta agcgccgaat
     5461 ccaactctgt cggtgccccg gtcagcttat ccagcggctt tttggcacgg ctgaggcacc
     5521 tccgtaagcc ctcctccgct tcgcgcctcg gaacggctgc ttcgagtgtc tccctcgtac
     5581 tcacgcgcat gaatgcgaac aggagccatg cggtctcgca aacgccaatt ctgctttcgc
     5641 gcggcaaacg cgcgctgctg tctgccacgg cttgcgcctg cacgacgctc gcgctcatcg
     5701 ggtgtaatcg ctccaattcg agttcgctga gtaaattggc ggatgacaat aacgttccgg
     5761 agttccccgc tcctgcggag aatgggccca aattgggttg tgtcgcaaac gccacaccca
     5821 tcttcgagaa ggccaccacg cattcgaagg tgattgggtt tttgcacgcg ggcgcgagtg
     5881 tcgcgcgcag cgaggcgccg gtccgcaaag cgcgagactg cgaatctggc tacttcgcga
     5941 tatttccacg cggtttcgtg tgcctcaacc aaggggccac actggacatg gcccatccga
     6001 ccctcgcggc gatggcaatt cagcctgctc tcgatcgttc gctcccatat acctacggac
     6061 gcgcacgcgc gagcagtgca ctcctggaac gcgatgcccg acagagcgat gccgtggtcg
     6121 aagtccagaa gttcgcccag ggttccggcg cggccgtggt cggatcgtgg actgctcgtg
     6181 agctgagccg ggagacggag cgcgaaagag ttgagtcaca gaaatccggt gattcgagaa
     6241 aagactctga ctccgcggct gtcctcgcca aggaagtgct cgccaaagaa gtgcccgcgc
     6301 gggagcaaga cagtagccca ccgcaacgtc tcgccctgct cacgagcggg cgctttgcgc
     6361 gagcgcgcga cctggaagcc gcgcgccctt cgagttttgc cggtcagagc ctggctgaaa
     6421 acggggcggc gttgcccgtc gcgttcgtca tcaagcgtgg tgtcttcaag taccgagttt
     6481 ccggcgacgt gctggagaag gccgagcagc tagagtttca cgacaagttc ggactgacga
     6541 gtcgctaccg cacgcaggat gggaatcggt tttggctcgt tcagggtgag ccggagcgct
     6601 gggtgcgtga acgggacgtc acgcgcgcca gtgctcgctt gaagttaccg gagttcgtga
     6661 gcgcgccggg cgcgaacgac gcgcaacgct ggatcgacat ctccgtcggc tcgggcacgc
     6721 tcgtggcgta cgttgggaaa cgcccagtct ataccaccct cgtcagtgtg ggccgggacc
     6781 gcttggctga gtacgtaacg gatggggctg ccgttactcg tcaaggtacg ttcgaggtgg
     6841 tggcgaagca tgtgtcttgg ctcgatttgg gggccatcgg agtagccgcg atggcggcga
     6901 atcccaatga cgccgcatcg aaagcgaacg ccgacgcggg cggcaccgtg cgcaatgccg
     6961 cgttcgcgag cgacacgaac aacgccgctg cgcccgctac ggattcgggc ccgaccaccg
     7021 cgggtctgaa tccagtcgcg agtattcagc tgttctccag gctctcggat cgctcgagta
     7081 agctcgcctt ctatgacgtg ccatggctgg tggagctctc ttccggtcaa ttcttgcatg
     7141 gcgcctactg gcacgaccgc tttggaatcg aacacggccc aggggacgtg gctctctcac
     7201 cgactgatgc gcgctggctc ttcgagttcg tgacacccac tctgcccaaa ggctggcacg
     7261 cggtcgggaa cggcagcgcg gacaaagtcg ccgccggcgg taaggcggtt ggagtcgatg
     7321 acgggggaag cgtcgagaag acgctggtcg tgattcacaa gtgatggaga cctcgagcgg
     7381 tgcgtccgcc gattcggtca ggcgatttgc tctaaaacgt ccatcgtggc gcgagaacgg
     7441 ttgagcgtat aaaagtggat gcccggcgcg ccgccttcga gcaacctccg gcactgcgcg
     7501 acggcatatt ccacaccgat gcgctggacg gcttccgtat caccgcccga tgcttcgagg
     7561 cgccccaata actccgaagg gatactggcg ccgcacatgg ccgtgaagcg tttgacctga
     7621 gatagattgg tgatcggcat cacgccgggc acgatcggta cccggacccc gcgctcgcgc
     7681 gcgcgctcca cgaaacggaa gtacacgttt ggatcgaaga acagctgcgt cagcagcagc
     7741 tccgctccga gctcggtttt gtgctttacc caatccagat ctcgctcgag gctggctgct
     7801 tccgggtgct tctcgggata acacgcggcg gccacacaga aggaccagcg cgacttgatg
     7861 aactgcacga gctccgacgc gtgggagaag ccgttggccg ccggcgtaaa ggtggtacta
     7921 ccttgcggcg gatcaccgcg cagcgcgatc acgttgtcga tttcgccgcg cgcgagttgg
     7981 tccagcactt cggcaatctc gtggcgctcg gccccgacac aggtcaggtg cgccatggtt
     8041 tcgagcccga gctcgcgctt gattctcttg accaagtcca cggtcaggcc acgcgtgctc
     8101 ccgaaggcgc cgtacgtcac cgagacgaac gcgggcgagt aacgcgcgag cttgccggca
     8161 gtctcgaata aacgctctac gccttgtgcg tccttgggcg gaaagaactc gaacgagaag
     8221 gccgggcggg ccgaggacgc gagcttgtcg gagatcttca tggcgacgag ggcgcgaata
     8281 ctacacgcag tcgggatttt tgccgctagt ccttcgtggc ggatcgaaaa acctcgctat
     8341 agttggccgg catgcacatc cacatcgtgg ccgtctcagg tacgggcatg ggatcgctag
     8401 ccgggttatt ggtcgagcta gggcacgaag tttccggcag cgacatcgcg ttcgatccgc
     8461 cgatgggacc ggcgctgcgg gagtacggcg tacgttgctt gactggcttc gacgcgcggc
     8521 acctggagcc cgcgcccgac ttggtcgtga tcggaaacgt gtgtcgaccg gacaatcccg
     8581 aggcccgcgc ggcgctcgag cggggcatcc cgtacacaca catcgcggga gcgctgtcgc
     8641 ggtttgcgct cgagaacacc tcgcccttgg tggtagcggg cacgcacggt aagacaacca
     8701 cgacggctct gtccgcgttc ttgctcgata agaccggctt tgcgccgggt tttctgattg
     8761 gcggtttgcc caaggacttc gcgcgcagct ttcgggcgaa agggccacgg cgcttgggcc
     8821 agctcccggc cgcggagggc catgcggcgc accgccgtaa gacgccattc gtgattgaag
     8881 gggacgagta cgatacggct ttcttcgaga agacggcgaa gtttctccac tatcggccag
     8941 aggtggcgat cctgacttcg atcgagcacg accacgtcga catctacccc acttggtcgg
     9001 cctacgtgga agcgtttaga cgcttcgtgg cgctgttgcc ggaggaaggc ctgttgatcg
     9061 cgaacgccgc agatcccgtc gtgcgagagc tcgcccgcga agctcgcgcg gaagtcgcgt
     9121 atttcgcgtt ggatggtgag gatacgggtg gaacggcgcc gcactggttg gcatcgccag
     9181 cacaagtggg gccgaccgga accagcttcg atttgtttgc gggcggggtg tcctgcggcc
     9241 gcttggcgtt gagtgtcccc gggcggcaca acctgaagaa tgcgctggcg gctttggcgg
     9301 cggcggccca gggctacggg gcggagctgc gcgagatcgg cccattgctc gctcagtttc
     9361 aaggggtgcg gcgtcggcag gatctgctcg gcacgccacg cggaatcttc gtctacgacg
     9421 acttcgccca tcacccgagc gcggtccgcg agacgttgct cgcgctgcgc caacgtcatt
     9481 cgacggggaa gctcatcgcc gtgttcgagc cgcgcagcgc gactgcgtgc cggaagctcc
     9541 accaagacga ctacccagct gccttcgggc ccgcggattt cgtactgctg gcgccgctcg
     9601 gtcgcagcca gctgccacca ggcgaagcgc tcgatttgtc cgtcctggtc agcgacctcc
     9661 ggaagcttgg taaggacgcc gaattggcac cggatctgtc cggaataccc cagcgaatcg
     9721 ccgagttggc cgcgcccggc gacgtggtcc tgctgctctc gaatggatca ttcggcaaaa
     9781 tcccggagcg cctgcccggt gtgctcgccg gcctgtccgc gacctgatat agagccgccc
     9841 gcgaggtgaa tagattgact tgcccctgtg gaaaaggtga atcgctcgag acctgttgcg
     9901 gcccgttctt gaacgggtcc aaaatcccgc aaacggccga agagctgatg cgttctcggt
     9961 acaccgcttt cgcggtgggg aatgtcgact acatcgtcga cacgcatgat cccgatcggc
    10021 gtggtgaagt cgatcggaac aacaccgagg cttggtccaa gcagtccgag tggttgggct
    10081 tcgagctcgt gaagtcggag ctcggtggtc cgagcgacga cgctggcgtg gtggagttcg
    10141 tggccaagta ccgcgccaag ggcgtgacga tcgcacatcg ggagcgttcg gtgtttcgga
    10201 agcagaacgg gcgttggtgg ttcgtcgacg ggcaaatcgt gaacggcccc cccgtgcgtc
    10261 gcgaagggcc gcgcgtaggt cgcaacgacc cgtgtccctg cggtagcggc aagaagtaca
    10321 agaagtgcca cggcgcggag gcatagggcg tgtgggcagg ctgccctgtc cccgccctgg
    10381 ttccacgtga agggggggcg agtgccagag cctagccgcg ttcaggctga cgaaccgagc
    10441 gcgattgaac ggcccacgct gctcgtgatt gggcgctccg atagcttcca agaagcgttg
    10501 gtcgagcgcc tttctcagcg cgctttgaat gttgaaacgg ctttgccgga gcgcgcggtc
    10561 gaagccacgg tggtgacggc tccggacctg atcgtgctcg ggcccgggct cggcgagaag
    10621 ggcttgctcg agcagctcgt gagttccgcc gtaagctcgg tgatcccgat cgtgctgctg
    10681 caggacgagg agccgctcga cgcgcgcttg cgggcttttc gtcgcggtgc ggccgcggtc
    10741 gtggcccgca ccgcgagcgt ggacgcgttg gcagagcgct tggccacgtt ggcgcgcgag
    10801 atcccggagc gcgacgggga cgtcggctcc gaagtcgtat ccgcgttggc gggtagcgcg
    10861 ggcgaggcga ccctcgagga gttcgtgcgc atgctcgcga gtgagctgcg ctcggggatt
    10921 ctctccgtgg agagcggggt ggtaaacgca ccggtgcggc tcgtgttggg cgccgggcgg
    10981 ccgttgctgt ccgcagtaga cgaattcgtg gcgcgcatct cgcagcacgt gctgcacgcg
    11041 gagcctctgc gctacgagtt cgatgatcgc gcgggtggca cgttggctgt gtgggacgag
    11101 ttgcctagtc gaggacgcct gggccgtggg gatgtgagtg ggctgcggct gatgttgtgc
    11161 gatgacgatc ctgcgcgcat cgatcaggtg gctcaacagc tcagagagcg cggcgctcac
    11221 gtcgcggtgt gctcattgtc tcccgacgcg ctggagctcg cggatctgcg gcagttcgac
    11281 cccgaggtgg tggtgatgag tgaggtcgcc atcgccggcg cgggttacgc gctgctgcga
    11341 cgaatgaagc aagatttgag gctacgctgg gcctcgctgt tggtggtgcg ttgggagcaa
    11401 atcttcgagc tcgatcgcac ggtgcccggc ttcgatcgtt tgacggccat gctcgcagac
    11461 ttggccgagg tcgatcgcgg tatgtcggat cgcgccgagc tcggcgatgc tttcgatgcg
    11521 cgcttggagg taagtggtcc cgcgcgttgc ttgcgcgcgc tcgcgcgcac gggtcgtgcc
    11581 ctcagtctga ccatccagca cgcgcgcgcc gagctgcggg tcgagctgtc gcgcgggctg
    11641 gtggtgagcg cttgggctcg ctcgcggcaa gactcgagca cgtgggaggg cgcgctcgcg
    11701 ctgagcgtca tgctgggcct ccgcacgggg cgggtgcacg tgacgcccat cgagcagcct
    11761 agcgccgcca atctcatggc ggaggtcgat ttggcgctgg attgggcgga gcgggagaca
    11821 tccccgctgg ctccatcgcg cccgagttcg gacgcgctgt ctgtcgcggg ggcccgcgct
    11881 gcagtggtgg tctcgccaac cttggcacca gctctggatc ctgcgacgca atcggcgttc
    11941 gaagtcgcga gcgcgacgtc ttggccagtg gcgga
//
LOCUS       DASTKU010000287         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1840954, whole genome shotgun sequence.
ACCESSION   DASTKU010000287 DASTKU010000000
VERSION     DASTKU010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840954"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1086
                     /locus_tag="VFQ61_10405"
     CDS             244..1086
                     /locus_tag="VFQ61_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9954907.1"
                     /translation="MHVTFYGVRGSVPAPGPTTARYGGNTSCVEVRLSDGSVLALDAG
                     TGLRALGNSLVREGKDARVHLLLSHTHWDHVMGLPFFAPLWKAGNHLVVYPLANDAQE
                     RFQRNIFDDIHFPVSVNDIPAKLELAKPDGTPWRIGSALVRPIQLNHPGGAQGFRIED
                     ADGSSLAYLTDNELNAETQVVATETLARFAEGVDLLIHDSQYLVSDMPHKLGWGHSIV
                     DDVLSLGLLAQPKRLALFHHDPDRSDEDLDALGQSSARWIRERTASVDLVVASEGLSF
                     RLGR"
     gene            1390..>2432
                     /locus_tag="VFQ61_10410"
     CDS             1390..>2432
                     /locus_tag="VFQ61_10410"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954908.1"
                     /translation="MKYGYFDDANREYVITNPKTPVKWINYVGTMRFGGIVDHTGGSL
                     LCKDDPALNRIVKYIPQLPASEFKGETLYVRVRDVGRDGSQRIFSPFFVPTLDPYDRY
                     ECHVGLGYNRYVSEFHGVRTEITVFVPYGAHTLVRAIKFTNLRQEPIELDAIPVVEYT
                     HFDALKQFNNADWVPQTMQSRALRESDGRVVLLQYPFMNKDRQVNYLTSNRPVSSFES
                     DRKRFLGDNEYGTFQRPLALLSDELENYEALRGDNVGALLHRLGRFESGETKTLIVQL
                     GQAESAEAARRASSQYLTDEAVESELRRQRVFWDEFLSKLQVKTPDKSFDSMVNVHNP
                     RQCHTTKNWSRYLS"
BASE COUNT          481 a          752 c          732 g          467 t
ORIGIN      
        1 gcccggcaag aggactcccg gtgagctcga ttcaacccga tcagccgtaa gcccagtcac
       61 ccactggacc caattcagcg gctcaaccca gtccgcgagc ccatcccggt caggcagaca
      121 gataagggat cggggcaccg agcaaaccgt cacccctatt cctgaccaag cttcccgccc
      181 agtggccgga cctcaccgtc cccctcgcag acccgccgcg cattggatag agtaaaggtt
      241 ccgatgcacg ttacctttta cggggtccga ggttcggtgc ctgcacctgg gccgaccacc
      301 gcccggtacg gcggcaacac ctcgtgcgtc gaagtccgtt tgtcggacgg cagtgtgttg
      361 gccttagacg cgggaacggg cctgcgagca ctcggcaact ccttggttcg agaaggtaaa
      421 gacgcgcgcg tccacttgct tttgtcgcat acgcattggg atcacgtgat ggggctcccg
      481 tttttcgccc cattgtggaa ggccggcaat cacctcgtgg tgtatccgct ggccaacgat
      541 gcccaagagc gctttcaacg caatatcttc gacgacatcc atttccccgt cagcgtcaat
      601 gacatcccgg ccaaactaga gctcgcgaaa ccagacggaa cgccgtggcg gatcgggagc
      661 gcactggtac gccccattca gttgaaccat cccgggggcg ctcaggggtt tcggatcgag
      721 gacgccgacg gttcatcgct cgcatacctg acggacaacg agctgaacgc ggaaacccaa
      781 gtcgtggcta ccgaaaccct ggcgcgattc gccgagggcg tagatttgtt gattcacgac
      841 tctcagtatt tggtcagtga catgcctcat aagctcggct ggggacattc catcgtagat
      901 gatgtgttgt cgctcggcct gcttgcgcaa cccaagcggc tcgcgctgtt ccaccacgat
      961 ccggaccgga gtgacgaaga cctcgacgcg ctgggacaaa gctccgcacg ttggattcga
     1021 gagcgcaccg cgtcggtgga tttggtggtt gcgagcgagg gtctctcttt cagactcggt
     1081 cgttgaggcc tgactagaac cgcagacgcg ctcacgctgg tgcgcagcgc gccgcggtta
     1141 gtgcgctgac gtgcaggctt gctcgatttc tagccggttc cgcgcgcgca gcagcgacgg
     1201 tgtgtggctc gagttccctc atttccgtcg acgagcggca acgaaaaatt gtgctgattc
     1261 ggcgagacgg ttggttggcg cggcaggtga ttcgggtaca cttcgcgccg ccgtacccca
     1321 tgggggtgac ggtcgctttc tgctgcaact tgcttcaaaa ccccccgccc cagtgtttat
     1381 ggcctcatca tgaaatacgg ctacttcgac gacgcgaatc gcgagtacgt aattacgaac
     1441 ccgaagacac ccgtaaagtg gatcaactac gtcggcacga tgcgcttcgg tggcatcgtc
     1501 gatcacacgg ggggtagcct gctctgcaaa gacgatccgg cgctgaatcg catcgtaaag
     1561 tacatccccc agctcccggc gtcggagttc aaaggcgaga cgctgtacgt gcgcgtgcgg
     1621 gatgtcgggc gcgacgggtc ccagcgcatc ttctcaccgt tcttcgtgcc gacgctcgac
     1681 ccgtacgacc gttacgagtg ccatgtgggc ttgggctaca accgttacgt gagcgagttt
     1741 cacggcgtgc gcacggagat caccgtgttc gtaccgtacg gcgcccacac gctggttcga
     1801 gcgatcaagt tcacgaactt gcgccaagag ccgatcgaac tcgatgctat ccccgtcgtc
     1861 gagtacacgc atttcgacgc gctcaagcag ttcaacaacg ccgactgggt cccacagacg
     1921 atgcagagcc gcgcgttgcg cgagagcgac gggcgcgtgg tgctgctcca gtatccgttc
     1981 atgaacaagg atcggcaggt caactacctg acctcgaatc ggccggtatc ttcgttcgaa
     2041 tcggatcgca agcgcttcct gggagacaac gaatacggga cctttcaacg tccgctcgcg
     2101 ctgctgagtg atgagctcga gaactacgaa gccttgcgcg gcgacaacgt cggcgcgcta
     2161 ctccaccgtc tgggccggtt cgaaagcgga gagacgaaga ctttgatcgt gcagctcggg
     2221 caggccgaga gcgcggaggc cgcccggcgc gcgtctagcc agtacttgac cgatgaagcg
     2281 gtcgagagcg agctccgacg ccagcgcgtg ttctgggacg agttcttgtc gaagctgcag
     2341 gtcaagaccc cggacaagag cttcgactcg atggtaaacg tgcacaaccc gcgtcagtgc
     2401 catacgacca agaactggtc gcgctacttg tc
//
LOCUS       DASTKU010000288         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_502820, whole genome shotgun sequence.
ACCESSION   DASTKU010000288 DASTKU010000000
VERSION     DASTKU010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502820"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFQ61_10415"
     CDS             <1..181
                     /locus_tag="VFQ61_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HET9954909.1"
                     /translation="GEAREVELFVMVLGASSYTYAEATLTQRLPDFVGSTIRGFEYFD
                     GVPEMLVPDQLRSAV"
     gene            227..427
                     /locus_tag="VFQ61_10420"
     CDS             227..427
                     /locus_tag="VFQ61_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954910.1"
                     /translation="MAQHYGVVVMPARPRRPKDKAKVEVGVQVVQRWIVARLRNRKFF
                     DLGELNQAIWELLEELNARPFQ"
     gene            590..1090
                     /locus_tag="VFQ61_10425"
     CDS             590..1090
                     /locus_tag="VFQ61_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954911.1"
                     /translation="MHAEVEVCATATLVEIFQAGERVASHRRSLERRGSTVTEPGHRP
                     ANHRHQVWPPERLIAWGASFGPSVARVVELTLGRYVNPEQGYRACLGLMRTAQRHGAA
                     RMNAACERALSVNIVGGPRRKYIEAILKRGLEREGVPVAPTPRTSPLTHENVRGGDYY
                     DKETVH"
     gene            1087..1854
                     /gene="istB"
                     /locus_tag="VFQ61_10430"
     CDS             1087..1854
                     /gene="istB"
                     /locus_tag="VFQ61_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HET9954912.1"
                     /translation="MTTIEETLTKMTELRLTSMAMAVRELMQTAPGHQLSFEEKLGIV
                     VDREWTERDNRRLARRLKDAKLVTRASLENVLCDPERGVDKATIRQLATGKWIQAKHN
                     IIIVGKTGVGKSYLAAAVADSACRTGNRAAFFRVPRLLEELALARAAGEYGALLGKLS
                     RVHVLVLDDFLLNPMTDAERRDLLEVIEDRYDRSSTVITTQVPTKTWHEALVDPTIAD
                     AICDRLVHNAQLLKLGGPSLRRKNAIPPEKTATQNPS"
     gene            complement(1925..>2144)
                     /locus_tag="VFQ61_10435"
     CDS             complement(1925..>2144)
                     /locus_tag="VFQ61_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954913.1"
                     /translation="RCLLACSFDDQLRLPRGSGKLGCDCSIGLCTFLVQGLPGARALR
                     CDGPPCTFQSPLQVQDRPILRIPVDLST"
BASE COUNT          435 a          677 c          694 g          338 t
ORIGIN      
        1 gggggaggcg cgagaggtgg agctcttcgt gatggtgctt ggcgcgagca gctacaccta
       61 cgcggaggcg acgctgacgc agcgactgcc ggacttcgtg gggtccacga ttcgcgggtt
      121 tgaatatttc gacggtgtac ccgagatgct cgtgcccgat caattgcgca gcgccgtgta
      181 gggccctgat cgctacgagc cggacatcaa tgcgacgtac ctggagatgg cgcagcacta
      241 cggggtggtg gtgatgccgg cgagacctcg acgtccaaag gacaaggcca aagtcgaggt
      301 cggcgtacaa gtggtccagc gctggattgt ggctcgtttg cgcaaccgca agtttttcga
      361 cttgggcgag ctgaatcaag ccatctggga gctgctcgaa gagctcaatg cgcgaccatt
      421 ccagtagctc gagggcagcc gcgagtcggc tttcgcagct ctcgacaagc ccgcactgcg
      481 aacgctaccg gcgctgcgct atgagctcgc cgagcgcaag accgcgcggg tcaacatcga
      541 ctaccacgtc gagtacgatg gtcgctacta cagcgtccct tacgagcgcg tgcacgccga
      601 ggtcgaagtc tgcgccacgg cgacactggt agagatcttt caggcgggcg aacgagtcgc
      661 gtctcatcgg cgcagtctgg agcggcgcgg gagcacggtg acagagcccg gtcaccgccc
      721 cgcgaaccat cggcatcaag tgtggcctcc cgagcggctg atcgcctggg gcgcgagctt
      781 cggtccgtcc gtggcccgag tcgtggagct gacgctgggg cgctacgtca acccggagca
      841 gggctaccgg gcctgtctgg gattgatgcg aacggcccag cgccacggtg cggcgcgcat
      901 gaacgcggct tgcgagcgag cgctcagcgt gaacatcgtc ggcgggccac ggcgcaagta
      961 catcgaggcc atcctcaagc ggggcctcga gcgcgaagga gtaccggtcg cgccaacgcc
     1021 ccgcacttcc cccctcacgc acgagaatgt gcgtggcggt gactactacg acaaggagac
     1081 cgtgcattga cgaccatcga agaaaccctg acgaagatga ccgagctgcg gctcaccagt
     1141 atggccatgg cggtgcgaga gttgatgcag accgcgccgg gccatcagct atccttcgag
     1201 gagaagctgg gcatcgtggt tgaccgcgag tggacagagc gtgacaatcg gcgcctcgcg
     1261 cgacgtctca aagacgccaa gctcgttact cgcgcctcgc tcgagaatgt cttgtgcgat
     1321 ccagagcgtg gcgtcgacaa agccaccatc cgccagctcg ccaccgggaa gtggatccag
     1381 gccaaacaca acatcatcat cgtgggaaag accggcgtag gaaagagcta cttggccgca
     1441 gccgtcgccg actcggcatg tcgcaccggc aatcgggctg ccttctttcg agtcccgcgg
     1501 ctgctggaag agctcgcact ggcgcgagct gcaggtgagt acggcgctct cctgggcaag
     1561 ctctcgcgcg tgcacgtcct cgtactggac gacttcctgc tcaatccaat gacggacgcg
     1621 gagcgtcgag atctgctcga ggtgatcgaa gatcgctacg atcgaagcag caccgtgatc
     1681 accacgcagg tgccgacaaa aacttggcac gaggcactcg tggaccccac tatcgcggac
     1741 gccatctgcg accgactcgt acacaacgcg cagttgctca agctcggcgg cccatcgctc
     1801 cgtcgaaaga acgccatccc gccagagaaa accgcaaccc agaaccccag ctgaccccag
     1861 accctcgtcg ctgcgctccg gcagtgctcg agtccgtcgg aacggctgct caagtccgcc
     1921 cggattaggt gctcaagtcg accggaatgc gcaatatcgg ccggtcctgg acttggagcg
     1981 ggctctggaa ggtacacggc ggaccatcgc atcggagagc gcgagcgcca ggcaaaccct
     2041 ggaccaagaa cgtgcacaga ccaatgctgc aatcgcaacc caacttgccg ctgccgcgcg
     2101 ggagtcggag ctgatcgtcg aacgagcacg cgagcaagca ccgg
//
LOCUS       DASTKU010000289         5541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1841153, whole genome shotgun sequence.
ACCESSION   DASTKU010000289 DASTKU010000000
VERSION     DASTKU010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841153"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1113)
                     /locus_tag="VFQ61_10440"
                     /pseudo
     CDS             complement(55..1113)
                     /locus_tag="VFQ61_10440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
     gene            complement(1188..1946)
                     /locus_tag="VFQ61_10445"
     CDS             complement(1188..1946)
                     /locus_tag="VFQ61_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HET9954914.1"
                     /translation="MSDGSAIEWTDATWNPVRGCTKISPGCKHCYAQTFSERFRGVPG
                     HPFEQGFDLRLVETALELPVRWRSPRRIFVNSMSDLFHEEVPEDFIRRVFDVMSRASQ
                     HQFQLLTKRSKRMAEIAGRLQIPRNVWMGVSVENERYVRRLGELRTVNAAVRFVSVEP
                     LLGPIRHLPLDGIHWVIVGGESGPRARPMNPVWVRAIREQCQAAGVAFFFKQWGGVQK
                     SRTGRVLDGRTWNEMPEYKLQLVSKERSLAVVNG"
     gene            4293..4913
                     /locus_tag="VFQ61_10450"
     CDS             4293..4913
                     /locus_tag="VFQ61_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954915.1"
                     /translation="MSTFEELASELTLRDSARAILRRHLLPEFGALPIDQLDVERIER
                     FKAERMAAGTHPKTLNNLLVVIRAVLQLAVERGAIAAVPIVRSLPLAERARAALPLDQ
                     ARALARVTEPVFHTMALLALLCGLRLRELRNLRWSDLQLEKATLRVGTREFPLTRPLL
                     LALSEQPQLSPWLFTLDGNQLTHGSAGKYCATPSIQYLFDLSLYSV"
     gene            complement(5292..>5541)
                     /locus_tag="VFQ61_10455"
     CDS             complement(5292..>5541)
                     /locus_tag="VFQ61_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954916.1"
                     /translation="IQGGHQVPIRKIVDRWARSIANAASIAAEVDRLYLYDNSVDDRD
                     AQLVIRATAGHVVRQYAEPSLWMGPIIQRLSTEDVNFE"
BASE COUNT         1094 a         1812 c         1550 g         1085 t
ORIGIN      
        1 gtcggtaagt cgataccgag cagcggattg agcatctttg ccgccggcag ggcttcacgg
       61 ggcgaagcgc agcctaagtt gtggatcagc gtacgtccct ggtcgacgag tcgccggcgg
      121 cgagaccgcc gagatctggc cagtgcgctc aagagcggcc aatacctgct tcttatagtg
      181 cgtttctcgg taaggcgtct ccgcgagcac gaagtcctcg accgccgcga ccgtcgcttc
      241 ggtgccggcg aaccgcccgc gtatcgctcg ctgaagcgcg gcaaaatccg gctggggact
      301 gaatagaagc gtctggcctg ggttggtagc atccgagaat cgaaactcgc cggactggtc
      361 gactttccac atggcctcct tcatcttcgc catgcccttc ggactgttcg tggcgaagaa
      421 gagaaagtag tcagtcacgt cgttgtcgtt tcgcatttcg aacgagcgca cgtactttgc
      481 tccagcggct gtcctcagct ggcgcaggta caggccgtgg aggaactgct tgcgcgcctt
      541 cggctccacg atcgcctcca tctggcgcca atcgccggtg ccaaacagcg cgtcgaagtt
      601 ctcctgctgg tcgggatgcc caatgaagcg gttgatttcc tcgaacatga agttgatgaa
      661 aacctcgcaa ctcgggttgg cgaggatctc cttcaccaaa tcgaacggca cgccagtcca
      721 cccgaaaggg tctataaacg cgaacgtcgg tggcagcgga cgattctgct tcttgtagaa
      781 gtcgagtagg tgctctcgaa acccttgttc gaaagtcttc tcccccacga ccttcgctcg
      841 aaaattggtg ggcaggctca gggcgatcac gagttcttcg agcttcgccg cacggtcggc
      901 gtccttctcg atgaacaaga agagcagcgt ggcactgatc ttcgactgat actgaagcgc
      961 tgccttgagc gcgatgatcg gggagccatc ttcgcccccg gagtaaaggc caggcccagc
     1021 gaacccatcc acgtaagcga tggtcgggaa gctgcccaag ctcaggatgg gtgtccacgc
     1081 atctagatag cgacgtagga tctggtgctt ggcccgcgtg tgcggatcga gagctcaaac
     1141 tgtcgttttc ggcgccgaca ttccccgctc ctttgctcgg tattcttcta cccgtttaca
     1201 actgcaaggc tgcgctcttt cgaaacaagc tgcagcttat actccggcat ctcgttccac
     1261 gtccgaccgt cgagaacgcg tcccgtgcgg cttttctgaa cgccccccca ttgcttgaaa
     1321 aagaacgcga ccccggcggc ttggcattgc tcgcggatgg cacgcaccca taccggattc
     1381 atcggacgag cacgcggacc actctcgcct ccgacaatca cccaatgaat cccgtcaagc
     1441 ggtaggtggc ggatgggacc gagaaggggt tcgacgctca cgaaacgcac ggccgcgttc
     1501 acggtgcgta gctctccgag gcgccgaaca tagcgctcgt tctccacgct gacccccatc
     1561 cacacgttgc gcgggatttg gagacgaccc gcaatctcag ccattcgctt cgatcgcttt
     1621 gtgaggagct gaaactgatg ctgcgacgcc cgagacatca cgtcgaagac gcgcctaatg
     1681 aaatcttcgg gcacctcctc gtgaaagagg tcggacatgc tgttgacgaa gatgcgccgc
     1741 ggcgagcgcc agcgcacggg cagctcaagc gcggtctcca ccagccgaag atcgaagccc
     1801 tgctcgaagg ggtggccagg gacacctcgg aaacgctcag agaacgtctg cgcgtagcag
     1861 tgcttgcagc cgggcgaaat cttcgtgcaa ccccgtacgg ggttccacgt cgcgtcggtc
     1921 cactcgatgg cacttccgtc agacaccaat cacctgaaca cgtgcacagt atcctattta
     1981 ccgcgcaaag caagccagaa aggcccctca aagaggccga gttcacgcga ttccggatgc
     2041 acatgcaagg gcctatcgcg cgagcctggc cgtttgcgcc cgagtcgttc gccacgatcg
     2101 cttccacgag gtcggcggtt cggcgagctg cgctcggcgt ccattgcgaa ccaatccgat
     2161 cgcaatgtga caacgcttcc agcttcgtcg gcaaacggtc tcattccacg acgcgagcgc
     2221 gacaacgcgc gctctgggcg gctaaagtcc atgcgtgttg cgtcacaata tctgagccgg
     2281 aaagttgcgc ctgtcccggt gcctctgcag ccgagcggcg tgaagtcgag cgccccggac
     2341 gctccgttga aacgaggcac tcgccgcgcg catctgagct atcccgctcg cacgagcgca
     2401 gccgctcccg cagtagcgat agagacctta ccatcgcgcg accacgcggc gccgcttgaa
     2461 ggctcggagt gcaatccggg ctttcgcctt acttggcatg ctcattctgt ttcggcggcg
     2521 tcagtccacg tgggccactt tggtgcggag atttggcggc caccgcgcta tcagccccaa
     2581 ttacgtggat tgggagagag ccagcgaccg ctccagcagg tcatgaaccg ccgatgccgt
     2641 tagaaacact ccccttagcg agtgcacggc ggtgcacgct tggcgccttg cgtcggcacc
     2701 ggacgcgtat ccaagtcctg ctgcagctgc gcgcgcaagc gcgccaggcc cggatgctcc
     2761 gagatttggt tccgccccct aagcccgccc tgaatccatc gcgcctatgc tttcccgctt
     2821 tcaaatgtcc ccaatggcat gccggccagt cttgcagcca ccccgtgacc gctcattcac
     2881 catactctaa acccagcagc gctctcccaa ccggcgctac tcaattacct gcgttgcagg
     2941 gcgtagcccg caaaaggcat ttggcacaca atcgctctcc gcagtcgctc tcccgcagtc
     3001 gctgggtaga gaatcagttc cacaaaaccg atgcagacgc ccatgacacg ggcccctgcc
     3061 cctccttggg tctccgaacc ggcacaaacg caatctcaat atctcgaaag cccgctcgtg
     3121 cataccgccg caaacctcct tgcactacca acatggctag caaggccgtc tattcaaccc
     3181 gcgtcgatct tcgtttccca ttcgcgagtc atcgctactc agccgccctt cggcccagtg
     3241 aattctccta ctcgagagag aatccgtatc aattcacgct gaccggagtc tcctgcttcc
     3301 ccagggcgcg ctcctggtcc cgcgggggta caatcagcgc tgctggcgta gctctttgag
     3361 tagtcacggg tgaaccgtcg cgctgcggtg cggatgctcc ccgagcgacg gtcgatatcg
     3421 agtatcgaat caacactcac cctcccctga cggttccacc gtcaccctgt catccacgcc
     3481 gaccaaaaca tgacgggtca accgtcaccc tatcatcgac gccgaccgaa aagtgacggg
     3541 tcaaccgtca ccccatcatc cacgccgacc gaaaagtgac gggtcgaccg tcacctttgg
     3601 gtcgaccccc atccaaaatt cgctcacggc tgctctcacg gagtctcgga gaggaccgaa
     3661 tcaactgcgg gctgcagaaa atgaaaagga agtcttgtcg ccagggcagc cgggtactgt
     3721 ttttgagatt cgctatcacg ccgatctgca gccgggagct gactccatat tgagaattga
     3781 gtgatttctt aggggccgat cgtcgaattg gcttatttta atccgatctg ggcacacccc
     3841 gcccgtcgca cgcattccaa tctcgttaat acaaacacac ttccttcgac acttgcgcat
     3901 ccgaagttac aaaagtcagt gtcacgggtc cattcggcta gtcagcgttg ttgcgggcga
     3961 ccgtctactc ggggtgtagg acacccgcac ttgaggactg cacccattct ttaactctcc
     4021 ctcacttccc agttgcacat cccactgctc gcacgtatag gagagaaagt cgcccgaggg
     4081 ccgcgttgga gcgcgacccc cgagctcacc caacacagga gtatacgtcc tatgcgggct
     4141 aaagatcagg gtaggtgtac gcgtgcgcgc acacaagggg ttcgcacgcg ttttctcgtc
     4201 catgttcgaa atgggagcca gaagccgccc aagcggtcgg gccgaaggct ccgtgacggt
     4261 tcgaccgtcc cgtcgcaagg agggcggttg cgatgagcac tttcgaggag ctcgcgtccg
     4321 agctcacact ccgtgactca gcgcgcgcca tcttgcgacg gcatctctta ccggagtttg
     4381 gggcgctccc gatcgaccaa ctcgacgtcg agcgcatcga gcgcttcaag gccgagcgaa
     4441 tggcggccgg cacgcatccc aaaacactca ataatctgct cgtcgtgatt cgcgccgtcc
     4501 tacagctcgc ggtcgaacgg ggagccattg cagccgttcc catcgtgcgc agccttcccc
     4561 tcgccgagcg cgcccgcgcc gcgctcccgc tcgatcaggc acgagcgctc gcccgtgtca
     4621 ccgaaccggt ctttcacacc atggccctgc tcgccctcct gtgcggcctg cgattgcgag
     4681 agctccggaa cctccgctgg agtgatctcc aactcgagaa ggccaccttg cgagtcggca
     4741 cccgggaatt cccactcact cgcccactcc tactggccct ctccgaacaa ccccaactca
     4801 gcccttggct attcaccttg gacggcaacc aactcaccca cggctcggct gggaagtatt
     4861 gcgcaacacc ttcgattcaa tatctcttcg atctctcgct ctacagcgtt tgattctgca
     4921 tactcacgga gaacgggcgc cagacggacg ttgagaccct tcatgactcc gtaagttcca
     4981 cggcggggcg gcacacgtcg gtgtcgcccc ggtcgtccct cggtggcggt ggttccgagt
     5041 cgtgaacctt tgcttgtcga cgagcggcgc gatccgcgcg caattgatga agctcgacac
     5101 gccgcccccg cttgcgcaga ctcgcccgaa cgctcgatgc ggattggggt cagccgaaag
     5161 ctcagggctc cgtgccgtct cgccccgtaa tggccaccag tggaacgaat gcggcccact
     5221 cgcgagtatc acagccccat ttgcaaccgg caggttgagt tcgaccgcaa ctgaatggcc
     5281 ggaccgaacg attattcgaa attgacgtct tcagtgctga gccgttgaat gatagggccc
     5341 atccatagcg aaggctccgc gtactgtcga acaacatggc cggcggttgc acggattacg
     5401 agctgagcgt cgcggtcgtc gaccgagttg tcgtagaggt agagacggtc gacttctgcc
     5461 gcgatggacg cagcattggc aatcgagcgc gcccagcgat cgacgatttt gcggatcggc
     5521 acttggtgac caccttggat g
//
LOCUS       DASTKU010000290         9068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_278073, whole genome shotgun sequence.
ACCESSION   DASTKU010000290 DASTKU010000000
VERSION     DASTKU010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278073"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..2301)
                     /gene="pta"
                     /locus_tag="VFQ61_10460"
     CDS             complement(151..2301)
                     /gene="pta"
                     /locus_tag="VFQ61_10460"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET9954917.1"
                     /translation="MPHPIYLAPSGSQVGLTSVALGLVRSLDKRGMRVAFFKPIAQME
                     RNQHERETGPERSTYFIRKTTSLQPTDPIPLETAERLLLAHRTDELLERVLRDYHVSA
                     RDADVVVIEGLVHSPNLPFEVELNLELIQALGADVVLVASQGQNTTADLDSLLELSAE
                     QYGGEHGTRLVGCIVNRVPRNDSNTPEDYLRETYQRSQFIHSGKIRLLGAIPNEPDLS
                     ACRTVDVARHLGAEVLFEGELTKRRVKRFTLLARTVPNLLYDLIPGSIMVTPADRSDI
                     ILATALAALNKIPIAGLVLTGDMPLPEAVLKLCKPAIATGLPVLRVRTDSWQTSLSLF
                     RMSSEVPVDDLERVQRSMDLIAEYVDPGLVTRRSAAPIEARLSPAAFCYRLTEWAKRA
                     QARIVLPEGTEPRTLRAAVICAQRGIARPVLLGDPAEVRQVAEGLELELPTNLEILDP
                     SKIRERYVDPLVEMRRTKGLLRGAAAELLEDNVWLGTVMLALGEVDGLVSGAVHSTAN
                     TLRPALQIIKTKPGARVVSSVFFMCMPEQVLVYGDCAVNPDPDAETLSDIAIQSAESA
                     LQFGLAARVAMISYSTGDSGSGSDVDKVRAATQLAKQRRPELEIDGPLQYDAAAIADV
                     ARQKAPTSPVAGRATVYVFPDLNTGNTTYKAVQRSAHVISIGPVLQGLNRPVNDLSRG
                     ALVEDIVYTIAITAIQANAETEALMAAAPKSASQ"
     gene            complement(2363..3541)
                     /locus_tag="VFQ61_10465"
     CDS             complement(2363..3541)
                     /locus_tag="VFQ61_10465"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET9954918.1"
                     /translation="MQILVVNCGSSSLKLSVVEPASGDSVLSGLAERLGTEQAVLTWK
                     TPGAGSRTLAVPGETHAGILKRAWSEVDASRIAAVGHRVVHGGEAFQESAVIDAAALA
                     AIEAASPLAPLHNPANLAGIQAAMQSFPKLVQVAVFDTAFHQTLPEAAFLYAIPYELY
                     TKHRVRRYGFHGTSHRYVAHIAAERLERPFDEVNLITAHLGNGCSVCAIRAGRSVETS
                     MGLTPLEGLVMGTRSGDVDPNLHVFLRDQAGLGLDQVIDLLNKKSGLLGLSGESNDMR
                     TLLDLEQRGHARAHLAIEVFCHRLAKCVLACGAGLERLDALVFTGGIGENAAPIRARV
                     LERLGLLGARLDPELNAQHGRTSGGRITLPDSQLVSMVVPTNEELIIAREAARFCEAV
                     "
     gene            3740..4054
                     /locus_tag="VFQ61_10470"
     CDS             3740..4054
                     /locus_tag="VFQ61_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954919.1"
                     /translation="MMQDIRYEQRPDGKLAGWFASPADKVLLRKCSDVLVSQFGAEPC
                     EHFDALDQSFWDFNVAALRITLHLDRAVGLAVVSNDATSPAHNLVRRFAEHLHALLLR
                     GL"
     gene            complement(4040..5578)
                     /locus_tag="VFQ61_10475"
     CDS             complement(4040..5578)
                     /locus_tag="VFQ61_10475"
                     /inference="COORDINATES: protein motif:HMM:NF017730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HET9954920.1"
                     /translation="MARLEFEFRFKPGDPGARRRAPGEPFRLLILADFSGRTKLDRVA
                     PKPVKVDAEHLDRALSRFEARVQLELPGVGPVTFSPRGLEELHPDGLLRSLSIFQEPL
                     RLRRAIAQGNASAEVLEQARAFLRSQGAAAAEVPVANAPTAATTPASAKPAENEHDTL
                     ARLLGGIGGKGAPGGEAAAPRAPSALQTIIERAVKPHLAEGPSLERDVVVRELDRVVN
                     NLLQAILEDPRYRSLEVAWRGAERVVRALDTDEIIEIALLDLSQDELRAAFGTSGELG
                     TEEERARAELVQRLPQETRYTCFAAALSFGPDDIASLAGLASWALRSGAFLLADAAPS
                     LLGIKTPSAAGDPRLWDPEPLPALWHALRASSLGKHVGLVYPRVLLRLPYGKTTDPVE
                     GIAFEEANGSGSSVARCFGSGALTVCEMLGRGYREYESDYLQSFEPELTDLPAYTYLD
                     DGEKKLVPTTEVTLSERAMAACLEEGILPLIPRRDRASVQLPQLASLASPAAALSEVS
                     SESA"
     gene            complement(5751..7037)
                     /locus_tag="VFQ61_10480"
     CDS             complement(5751..7037)
                     /locus_tag="VFQ61_10480"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HET9954921.1"
                     /translation="MTNHRELTTGRLGRLARLAGLGARTGLSLVASRGVEAAAAQAAE
                     VLGSLRGLAAKAGQMASYIDGFVPEGQRAAYEAALSSLRRSAPTSSPEEIRALFESDL
                     GAPTRELFEQFSEEPFASASIGQVHRARLRDGRDVAVKIQHPGIDRAVESDLENASVL
                     RSVIGAALPRGTDTRRVFEEIRARFREELDYRLEAERQAAFAELFASDEAVRIPAVVA
                     SHSSRRVLTSELAHGETLEQAALRSEAERAAYARTLWHFVFKGVLVGRMFNADPHPGN
                     YLFGEGGQITFLDFGCVELISEDRLAPARSMHVAAILRDEERFKDGVRRLLATRGGNY
                     ERVVLEYVRHTFTPMFESPFNITPEYVTSVVQGVRALKSQMLARDSSFVMPPPGLTMM
                     NRLQFGFYSVLARLNVTVDYAAVEQALLQEAGVPIL"
     gene            7471..7971
                     /locus_tag="VFQ61_10485"
     CDS             7471..7971
                     /locus_tag="VFQ61_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954922.1"
                     /translation="MARFIKFASLLLSASIIVVGCGDDDDSNSTPPATKCKSPYAGAT
                     EAQLKAATTSGACQGDTEAICKNDMITLAATCGVSCVQTSSDALEQAACTQSCMKMNA
                     EPDPSDACLTCYMLSVQCAATNCFAECAADGNTPTCQSCRVEKGCTDAFYTCSGLPEP
                     TADGGV"
     gene            7968..>9068
                     /locus_tag="VFQ61_10490"
     CDS             7968..>9068
                     /locus_tag="VFQ61_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954923.1"
                     /translation="MKDREHRRHGRARPEFARYGAGAAIVLAGMSVCGVAHAAGYDTP
                     MLYSARHLGMGGSAIGYVDDPSALFHNPAGLAAVRHAGVLGDFSLLLGHVHGSPNVLA
                     QNRTSELTVAPFFLVGGAFRVHRLLTVGLGIYPIASSGATYEYGQPGFEDTTKLLFVE
                     GSLAVGVNLAPRLRLGLGYRVTFVELERFSGNRDDGTTPFLDFRLRGHNFGGFRVGLQ
                     FDATPDWKVGVSYRHQVTTRVENELGVALRAPYEDVHTEFLLPSKLGLGTRYDLDGTG
                     VPVSAALDLEYTFGSQNVGTPLIGVPRGGTDSIAVPNVFEWDDSLTARVGLEYRVGQY
                     LTRGARVATDAANLPDAGWAFRLGYVLDTRATN"
BASE COUNT         1654 a         2895 c         2936 g         1583 t
ORIGIN      
        1 gaggctcggg taccgggcgt gagcccgtga ttgggggcgg cgcgcgcggt gcccgggcgt
       61 gcttcgggcg cacacggctg cgccaggaag aacaggcggc ggtcggtcgc gcccgtaatt
      121 tcgaggccgc attctccgaa ccgtaaccga ctactgcgag gcgctcttgg gagccgccgc
      181 catgagcgct tcggtttcgg cgttggcctg aatggccgtg atcgcgatcg tgtagacgat
      241 gtcctccacg agcgcgccgc gagacaggtc gttgacgggg cgattcaagc cctgaagcac
      301 ggggccgatg ctgatgacat gcgcgctgcg ctgcacggct ttgtaggtgg tgttgccggt
      361 gttcaaatcg gggaacacgt acacggtggc gcgcccggca acggggctcg tgggcgcttt
      421 ctgccgagcc acgtccgcga tggcggccgc gtcgtattgc agcggaccat cgatctcgag
      481 ctccgggcgg cgttgcttgg cgagctgggt ggcggctcgc accttgtcga catccgagcc
      541 cgaacccgag tctccggtgg agtagctgat catcgccacg cgcgccgcga ggccgaactg
      601 aagggcagac tcggcgctct gaatcgcgat gtcggacaga gtttcggcgt ccggatccgg
      661 gttcaccgcg cagtccccgt acaccagcac ctgctcgggc atgcacatga agaacaccga
      721 cgaaacgacg cgcgcgcccg gcttggtttt gatgatttgc agcgcgggac gcagtgtgtt
      781 cgcggtggag tgaaccgctc cagagacgag cccgtctact tcgccgagcg ccagcatcac
      841 cgtgccgagc cagacgttgt cttccaggag ctcggccgcc gccccgcgca ggagcccctt
      901 ggtgcgccgc atctcgacca gcggatccac gtagcgctcg cggatcttcg aagggtctag
      961 gatttccagg ttggttggga gctcgagctc caggccttcg gcaacctgac gaacctcagc
     1021 cggatctccg agcaagacgg gccgcgcgat gccgcgctga gcgcaaatca cggcggctcg
     1081 taaagttcgc ggctccgtgc cctccggcag cacgatgcga gcctgggcgc gcttggccca
     1141 ctcggtcagg cggtagcaga aggcggccgg cgacaagcgc gcttcgattg gcgcggccga
     1201 gcggcgcgtg accagacctg ggtccacgta ctcggcgatc aagtccatgc tgcgctgcac
     1261 gcgctccagg tcatccaccg gcacttcgga gctcatgcgg aacagggaaa gcgaggtctg
     1321 ccagctgtcg gtgcgcacgc gcagcaccgg cagcccggtg gcgatcgccg gtttgcacag
     1381 tttgagcacc gcctcgggca gcggcatgtc tccggtcagc accaagccgg cgatcgggat
     1441 cttgttcaac gcggcgagcg cggtcgcaag aatgatgtcg ctgcgatccg caggtgtcac
     1501 catgatcgag ccggggatca ggtcgtacag caggttcggg accgtacggg caagcaacgt
     1561 gaagcgcttg acccgccgct tcgtcaattc gccttcgaac agcacctccg cccccaagtg
     1621 acgggcgaca tctacggtgc ggcaggcgct caggtcgggc tcgttgggaa tcgcaccgag
     1681 cagccgaatc ttgccgctgt ggatgaactg cgatcgctga taggtctcac gcagatagtc
     1741 ctcgggcgta ttcgagtcgt tgcgcggaac ccggttgacg atgcaaccga ccaaacgcgt
     1801 gccatgctct ccgccatact gttccgcact gagctcgagc aacgagtcca gatcggcagt
     1861 ggtgttctga ccctgagacg cgaccagcac cacgtccgcg cccagcgcct gaatgagctc
     1921 gaggttgagc tccacttcga acggcaggtt cggggagtgg accaggccct cgatcactac
     1981 aacgtccgcg tctcgagccg agacgtgata gtcgcgcaag acgcgctcca agagctcgtc
     2041 cgtccggtgc gcgagcagca atctctcggc cgtttccagc gggatcggat cggtgggctg
     2101 cagggaggtc gtcttgcgaa tgaagtaagt cgaacgctcc ggcccagtct ctcgttcgtg
     2161 ctgattacgc tccatctgcg cgatcggctt gaagaaggcc acgcgcatgc cgcgcttgtc
     2221 gagcgaccgg accaggccca aggcgaccga agtgagaccg acctgacttc cgctaggtgc
     2281 caagtagatg gggtgaggca tgtggagctc gcgtttaggc aggttcggca aagaggtacg
     2341 gttttctagc agcaatcggc gctcagaccg cctcgcaaaa tcgggcggcc tcgcgggcaa
     2401 tgatcaattc ctcattcgtg ggcacgacca tcgatacgag ttgcgagtct ggtagagtga
     2461 tccttccccc actcgttcgg ccgtgctgcg cgttcagctc ggggtccaag cgcgcaccga
     2521 gcaagccgag ccgctccaag acgcgtgcgc gaatgggcgc ggcattctcg ccgatgccac
     2581 cggtgaagac cagcgcgtcc aggcgctcga gcccggctcc gcaagccaac acgcacttgg
     2641 cgagacgatg gcaaaacact tcgattgcca agtgggcgcg cgcgtgtccg cgttgttcga
     2701 ggtcgagcaa cgttcgcatg tcgttgctct ctccagacaa cccgagcagg ccgctcttct
     2761 tgttcagcag gtcgatcacc tggtccaagc ctaggccagc ttggtcgcgc aaaaaaacgt
     2821 gcaggttggg atccacgtcg ccgctgcgag tgcccatcac caagccctcg agcggagtga
     2881 gtcccatgct cgtttctacg ctgcgacctg cgcgaatggc acagacgcta cagccgttcc
     2941 ccagatgggc ggtgatcagg ttgacctcgt cgaacggccg ctcgagccgc tcggcggcga
     3001 tgtgcgcgac gtagcggtga ctagtgccat ggaagccgta acgtcgcacg cgatgcttcg
     3061 tatagagctc gtacggaatg gcatacagaa aggccgcctc gggcaacgtc tggtgaaacg
     3121 ccgtgtcgaa caccgccact tgcaccagtt tcggaaagct ctgcattgcg gcctgaatgc
     3181 ccgcgagatt ggcaggattg tgcaatggag cgagcgggct tgcggcctcg atggcggcga
     3241 gggccgcggc gtcgatcacg gcgctctctt gaaatgcttc cccaccgtgc accacgcgat
     3301 ggccaacggc ggcaatgcgg gatgcatcca cttcggacca cgcgcgcttc aagatcccgg
     3361 cgtgggtttc gccgggcacg gcgagcgttc gtgagcccgc ccccggtgtc ttccaggtca
     3421 gtaccgcttg ttcggtgccg agccgttcgg ccaaacccga aagcacggaa tcgccagaag
     3481 cgggttcgac gacggacagc ttgagcgaag agctgccgca gttgacgacg agaatctgca
     3541 tggcggcaca accatacgcc gttcgtcact ggctgcatgc tcgcaggcga tatcgcgcat
     3601 gcggaaagtg cgtgtttcgg cgaaaccctg ctcgggtcac acaccgaaat gacgaaagcc
     3661 gcgcgggctg gctgaccggg ctgtcacccg tgatggttcc cgcaattggc tcgaaaccgg
     3721 cagggtttgc tagatgggaa tgatgcagga cattcgttac gagcaacgcc cggatggcaa
     3781 actcgcgggc tggttcgcct cgcccgcaga taaggttttg ctccgcaagt gctcggacgt
     3841 cttggtgtcc cagttcggtg cggagccttg cgagcacttc gacgcgctcg accaatcatt
     3901 ctgggatttc aacgtggccg cgctgcgtat cacgctgcac ttggatcgcg cagtggggct
     3961 ggccgtcgtc agcaatgacg cgactagccc cgcacacaac ttggtccggc gcttcgccga
     4021 acacttgcac gcgctgctgc tacgcggact ctgaggaaac ttcgctcaag gcagccgcgg
     4081 gcgaggccaa ggaggccagc tgaggcaact gcacgctcgc gcgatcacgc cgcgggatga
     4141 gcggcagaat gccttcttcc agacaggccg ccatcgctcg ctcggacaac gttacttcgg
     4201 tcgtcggcac gagcttcttt tctccgtcat ccaggtaagt gtatgcgggc aaatcagtga
     4261 gttcgggctc gaaggactgc aggtagtccg actcgtactc gcgatagccg cggcccaaca
     4321 tctcgcaaac ggtcaatgct ccgctgccaa agcagcgggc caccgaagaa ccggagccgt
     4381 ttgcttcttc gaacgcgatc ccttccacgg gatcggttgt cttcccgtag ggtaagcgca
     4441 acagcacccg cggatacacg agccccacgt gcttgccgag cgagctagcg cgcagcgcgt
     4501 gccagagcgc gggcaacggc tccggatccc agagcctcgg gtcgccggct gcgctggggg
     4561 tcttgattcc gagcaggctg ggcgcagcat ccgcgagcaa aaaggcgccg ctacgcaggg
     4621 cccaactcgc aagcccggcc aagctcgcta tgtcgtcagg cccgaagctg agcgccgccg
     4681 caaagcacgt atatcgggtc tcttgcggca ggcgctgcac gagctcggcg cgcgcgcgct
     4741 cctcctccgt gcccagctcg ccgctagtgc cgaaggcggc gcggagttcg tcttggctca
     4801 gatcgagcag cgcgatctcg atgatctcgt ccgtatccaa ggcccggacc acgcgctcgg
     4861 ccccacgcca agcgacctcc agcgaacgat agcgcgggtc ttcgaggatg gcctgcagaa
     4921 gattgttcac cactcggtcg agttcgcgga cgacgacgtc gcgctccaag ctgggtcctt
     4981 ccgcgaggtg cggcttcacc gcgcgctcga tgatggtttg cagagcgctg ggcgcacgcg
     5041 gggccgccgc ctcgccaccg ggcgcgcctt tgccgcctat cccaccgagc aaacgagcta
     5101 gcgtatcgtg ttcgttctcg gcgggcttcg cgctagccgg agtcgtggct gccgtaggcg
     5161 cgttcgcgac gggaacctct gctgcggcag caccttggct acgcaggaaa gcccgagcct
     5221 gctccagcac ttccgcgctc gcgttgcctt gtgcgatggc gcgcctcagc cgcagcggct
     5281 cttggaagat cgagagcgag cgcaaaagcc cgtccggatg aagctcttcg agcccccgcg
     5341 ggctgaacgt gacgggtccc acgcccggca gttcgagctg cacccgagct tcgaaccgac
     5401 tgagcgcgcg atctaggtgc tcagcgtcca ctttcacggg tttgggtgcc acgcgatcga
     5461 gcttcgttcg accggaaaag tcagccaaaa tcaacaggcg aaacggctcc ccgggtgcgc
     5521 gccgccgcgc gccggggtct ccaggtttga agcgaaattc gaactccagg cgtgccatag
     5581 ggctggttgt accccagctc cgtggcccaa gccgagcctg gatcgttcgg gagcgcacct
     5641 acggccgtgt tctcagccta gattcagcga ccgggagccg agcgcgtggg cgcgccagat
     5701 tgggcgagaa cgcgaagaat ctcctacttc ggggtcgcgg gcagtaaggc tcagaggatc
     5761 ggaacgcccg cttcctgcaa aagggcttgc tccaccgcgg cgtagtcgac cgtgacattc
     5821 aagcgcgcga gtaccgaata gaacccgaac tgaagccggt tcatcatggt caagcccggg
     5881 ggtggcatta cgaagctcga gtcgcgcgcg agcatttgag acttgagcgc gcgcacaccc
     5941 tggaccaccg aggtgacgta ctccggagtg atattgaagg gtgattcgaa catcggcgtg
     6001 aaggtatggc gtacgtattc gagcacgact cgttcgtagt tgccgccgcg cgtcgcgagc
     6061 aagcgcctca cgccgtcctt gaatcgctct tcgtcccgca agatcgcggc aacgtgcatg
     6121 gagcgtgcgg gggccagacg atcttcggag atgagctcga cgcaaccaaa gtcgaggaac
     6181 gtaatttggc cgccctcacc gaatagataa ttgccaggat gcgggtcggc gttgaacatg
     6241 cgcccaacca gcaccccctt gaacacgaag tgccacaaag ttcgcgcata cgctgcgcgc
     6301 tcggcttcgg agcgcagcgc cgcctgttcg agcgtctcgc cgtgggcgag ctcgctggtc
     6361 aacacgcgac ggctggagtg actggcaacc acggccggga tccgcaccgc ctcgtcactc
     6421 gcaaacagct cggcaaatgc ggcttggcgc tccgcttcca agcgatagtc caactcctcg
     6481 cgaaaacggg cccggatttc ctcgaacacg cggcgggtgt ccgttccccg cggcaacgcc
     6541 gcgccgatta cgctgcgcaa gacggaggcg ttctccaggt cactctccac ggcgcgatcg
     6601 atgccggggt gttggatctt caccgcgacg tcccggccgt cgcgcagccg cgcgcggtgc
     6661 acttggccga tcgacgcgct cgcaaacggc tcttccgaaa attgctcgaa cagctcacga
     6721 gtgggcgcac cgagatcact ctcaaaaagc gcgcgaattt cctcaggtga ggaagtaggc
     6781 gcggatcgcc gcaagctgct caaagccgct tcgtatgcgg cgcgttggcc ttccggcacg
     6841 aagccgtcga tgtagctcgc catttgtccg gcttttgctg ccaagccgcg caacgatccc
     6901 agaacctccg ccgcctgggc ggccgcagct tcgacaccgc gcgaagcgac cagactgagc
     6961 cccgtccgag cgccaaggcc tgccaaacga gccaaacgcc ctaagcgacc ggtggtaagc
     7021 tctctatgat tcgtcacgcc cgagtcatgg caccgcgcct cgcgttccga aagtggtccg
     7081 gtaccaggaa caggccggta ccaggaacag gccggtacca ggaataccag gaagaggcca
     7141 cggcgcaccg gaagcagcat ggcgcgcgag ctcctacgca cttgcccttc atgcgcaggc
     7201 gcggatacac tgggcgtttg aatgcgcgag ggataccgtc ttgtcggagc atttggcggg
     7261 agcgttcgct gaacgcatct gctgagagca agccatgcgg gctttgaccg tgattcaccg
     7321 ctcaccccta tcgaccgagc tacgcgccgg ggcgcatcca aacgagcgaa gcgccgttaa
     7381 cccagcgaaa gccctgaaaa gagcgaggca ctcgtaacct agacgagccg ccccaagacg
     7441 tttccgaacg tatcgagaaa gaggtttaga atggccagat tcatcaagtt tgcgagctta
     7501 ctactgagcg cctcaatcat cgtcgtgggc tgtggtgatg acgatgactc gaacagcacg
     7561 cccccggcga ccaaatgcaa atcgccgtat gcgggcgcaa ccgaagctca gctcaaagcc
     7621 gcgaccacga gcggggcttg ccaaggcgat acggaagcca tttgcaagaa cgacatgatc
     7681 acgctagccg caacttgcgg cgtgagctgt gttcagacct cgtccgatgc cctcgagcaa
     7741 gcagcctgta cgcagagctg catgaagatg aacgcagagc cagatccgag cgacgcctgc
     7801 ctcacgtgtt acatgctgtc cgtacagtgc gcggccacga actgtttcgc ggagtgtgcg
     7861 gcggacggca acacccccac ctgccagtcg tgccgcgtag agaagggctg cacggacgct
     7921 ttctacactt gcagcgggct acctgagccc accgcggacg gcggcgtatg aaggatcgag
     7981 agcatcggag gcacggccgt gctcggcccg agttcgcccg atacggggcg ggcgctgcca
     8041 tcgttttggc cggaatgagc gtgtgcggtg tggcgcacgc cgcgggctac gacaccccga
     8101 tgctctattc cgctcgtcac ctgggcatgg gcggcagtgc gatcggttac gtggacgatc
     8161 cctcggccct attccataac ccggcgggct tagcggcggt gcgccacgcg ggggtgctcg
     8221 gggatttttc cttgctcctc gggcacgtgc acggcagccc caacgtgctc gcgcagaacc
     8281 gtacctccga gctcaccgtg gcgccgttct tcttggttgg aggcgccttc cgggtgcacc
     8341 ggctgctcac ggtcgggctc gggatttacc cgattgcttc gtctggagcc acgtacgaat
     8401 acggacaacc cgggttcgaa gacacgacca agctgttgtt cgtggagggc tcgctggccg
     8461 taggcgtcaa cttggcgccg cggctgcgct tggggctcgg ttatcgagtc acgttcgtgg
     8521 agctcgagcg gttcagcggc aatcgggacg acggcactac cccgttcttg gacttccgcc
     8581 tgcgcggcca caacttcggc gggttccggg ttggcctgca gttcgacgcg acgccagact
     8641 ggaaggtcgg cgtttcgtat cgacatcagg tcaccacgcg cgtggagaac gagctcgggg
     8701 ttgcgctgcg ggcaccttac gaggacgtgc ataccgagtt tctattgccg tccaagctgg
     8761 ggttgggcac acgctacgac ctcgacggta ctggcgtgcc ggtgagcgcg gccctagacc
     8821 tggaatatac gtttggtagc cagaacgtcg gcactccgct gattggagtg ccgcgcggcg
     8881 gcaccgattc gatcgccgtc ccgaacgtgt tcgagtggga cgactcgctg acggcccggg
     8941 tcggcctcga atatcgagtc ggtcagtatc tgacgcgagg agctcgcgtt gctacggacg
     9001 ccgcgaacct gcccgacgcg ggttgggcct ttcgcctggg ctatgtgctc gacacgcgag
     9061 ccaccaat
//
LOCUS       DASTKU010000291         6364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1618566, whole genome shotgun sequence.
ACCESSION   DASTKU010000291 DASTKU010000000
VERSION     DASTKU010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618566"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..880)
                     /locus_tag="VFQ61_10495"
     CDS             complement(11..880)
                     /locus_tag="VFQ61_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET9954924.1"
                     /translation="MASPSTTADAVIPQRSSADDNYLVHDKGLLSWIFTLDHKRIGLM
                     YLASVLVAFALGGFFALAVRTELLTPGRTIMDADTYNQMFTLHGAIMVFLVIIPGIPA
                     ALGNIIMPLQLGAKDVAFPRMNLASYHLWVIGALFMLSSLAINAVDTGWTFYTPYSTS
                     TATAVIPAAFGAFILGFSSIFTGLNFIVTIHKMRPPGMTWFRMPLFVWALYSTALIQV
                     LATPVIGITLLMMIIERAFGLGIFDPALGGDPVLFQHFFWFYSHPAVYIMALPAFGVI
                     SEMIPPFARKPIF"
     gene            complement(896..1894)
                     /gene="coxB"
                     /locus_tag="VFQ61_10500"
     CDS             complement(896..1894)
                     /gene="coxB"
                     /locus_tag="VFQ61_10500"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET9954925.1"
                     /translation="MTRLSSLLLTVGEQPKNLPKVPESDTFWMPLDASVTTRSVDWIY
                     YFLYWMSVISCVLVFGAMILFIVKYRARSRSANQKVERTSDHSTALEVTWSVLPLFVV
                     IALFVWGFRGFVDLRTVPKNAIEIRATGQKWKWLFTYPNGYVDDQLHVPQDRPVRMLI
                     NSVDVLHSLYIPNFRTKMDAVPGRYTDLWFQANQPGEFPVFCAEYCGTSHSNMLTQVV
                     VHEPGGYEKWLNDASVWKGTPEELGANLYAKQGCNTCHSVDGSPLIGPTWKGLFGKTE
                     NTSAGAVKVDENYLRESIVDPTAKVVTGFTPSMPTYQGKLKDAELDGIIAYIKSLK"
     gene            complement(1891..2781)
                     /locus_tag="VFQ61_10505"
     CDS             complement(1891..2781)
                     /locus_tag="VFQ61_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET9954926.1"
                     /translation="MASWAASVRATLVVGLLSVVSSTTLVSSTVAAKPVPESERVERT
                     DPVPKQLQGVEVNERLGQVLPKTLGFRDHRGNDVVLGQYFDGKHPVILTLNYSNCPML
                     CSLQLSQLVNSLKQVAWGVNRDYRVVTVSLEPNEPLELQQRTESRYLTQYGRPEARGG
                     WAFLSGSSGNVRAYADAIGFSYRYVDERKEFAHPAAIVLAAPDGTIVRYLYGLQYDTQ
                     TLRLALTEASRGEIGSTIDKLILYCFHYDSSQGRYAPVAQKIMKLAGAVSVVLLAGFL
                     FVMRRSEGKRKRQLLESTAT"
     gene            complement(2786..3388)
                     /locus_tag="VFQ61_10510"
     CDS             complement(2786..3388)
                     /locus_tag="VFQ61_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954927.1"
                     /translation="MATEEDKVNTGVLMTLVGVGTLAVLGVAVALTALTRGELGEAAG
                     ERGESASYEYRALKKAQLDKLGQGTPIEQSMQQVVSRLKADPSSATPPGSASAAPAAN
                     SPADSGAQAPGGIPSSQPGATGAAPITGGTPPAVDGAPPTKNAKESGGAAPSPNTPGP
                     KAVSPQPVPPTHSQPTQPLAPTRPSPNPPAQPGSAPQPHG"
     gene            complement(3399..4718)
                     /locus_tag="VFQ61_10515"
     CDS             complement(3399..4718)
                     /locus_tag="VFQ61_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954928.1"
                     /translation="MATSKDSKDSKGPSSKAPAGKESTSKAAPARDAADEAADRFGDN
                     IRAGSLASGVLTKAGAAAAVGLGATVVLGMMQGDGFKRFSYSYLTAYLWALAIGLGAV
                     FWVTLQNLVNAKWSVALRRVGELLATSIPVLAILALPVVVPIFLGNDIMYVWANHETV
                     AGDHVLHHKAGYLNSGFFLVRFVFYFGFWTLLGRYFLKQSLLQDKSGAASIAERMQKV
                     AGPGMIAFALTLTFCAFDLIMSLDPYWFSTIFGVYYFASCVLAVHATLILVLMWLQGR
                     GRLSKSITPEHFHDLGKMMFAFTVFWAYIGFSQFMLMWYANIPEETAWYKERFAGDWG
                     GVSWALLILHFVVPFFGLLSRHVKRHRKALAFWAVWVLCEIYLDMYWLVMPNVQKEEL
                     HVHLMDITAWIGMAGAVVAALAFAAKNVNLVPVKDPRLPRSLAFENI"
     gene            complement(4753..5970)
                     /locus_tag="VFQ61_10520"
     CDS             complement(4753..5970)
                     /locus_tag="VFQ61_10520"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HET9954929.1"
                     /translation="MSDAQVKKTEGSPGEAPLHGLLAEYQSPWDLVDASKKVRDAGFE
                     RWDTYSPFPIHGIDNAMGIRMTVLPWLTLAAGLVGLFFAATLQYWTNGVDYPWIISGK
                     PFWSIPANVPIMFELMVLFAGITTVVSMLVLNGLPELSNPLDLKERFGRATDDRFFLM
                     IQASDPKFDGHKTRALLESTHPVVLDEVKEDRVTSNDPPKFIAYVMIIMTAAAAIPFA
                     MAAKARFSTSRSPRIHVIGDMDWQPKYKAQRRNPAFADERATREQVPGTVAVGEFRAD
                     DHFYTGKLAGAYATAFPAQVPASEATVARGRERFGIYCAPCHGLAGDGDGMIHKRAEE
                     LAASGRGKGMAWVQPSNVNQDYIKAQPVGQLFETISNGVRNMPGYASQIEPADRWAIV
                     MYLRALQNRSPNK"
     gene            complement(5986..>6364)
                     /locus_tag="VFQ61_10525"
     CDS             complement(5986..>6364)
                     /locus_tag="VFQ61_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HET9954930.1"
                     /translation="MITCNVIAPQFFWVKKLRRNPWVTLAIGMLINVGMWFERFVIIC
                     TSLTRDFLPSSWGYYIPTPIDFMTLGGSFGLFFTLFLLFCRYLPMVAISEVKAFHPSV
                     LAHAHGHGDHHDDHAGQPAHAGH"
BASE COUNT         1235 a         1958 c         2045 g         1126 t
ORIGIN      
        1 accacgctgg tcagaagatc ggcttgcggg cgaacggagg gatcatctcc gagatcacgc
       61 cgaaggccgg cagcgccatg atgtacacgg cggggtgcga gtagaaccag aagaagtgct
      121 ggaacagcac cgggtcaccg ccgagcgccg gatcgaagat accgaggccg aacgcgcgct
      181 cgataatcat catgagcagg gtgatgccga tgacgggcgt cgctagcacc tgaatcaagg
      241 ccgtggaata cagggcccag acgaacaacg gcatgcggaa ccaggtcatg ccgggcggac
      301 gcatcttgtg gatggtcacg atgaagttca gccccgtgaa gatcgagctg aagcccagga
      361 tgaacgcacc gaacgcggcc ggaatcaccg cggttgcggt agaggtgctg tacggcgtgt
      421 agaaggtcca gcccgtgtcc accgcgttga tggcgaggga cgagagcatg aacagcgcgc
      481 caatcaccca caggtgatag ctggccaggt tcatgcgcgg gaacgccacg tccttggcgc
      541 ccagctggag cggcatgatg atgttgccca gcgccgccgg gatgccgggg atgatgacga
      601 ggaacaccat gatcgcgccg tgcagcgtga acatttggtt gtaggtgtcc gcgtccatga
      661 tcgtgcgccc gggcgtgagc agctcggtgc gcaccgcgag ggcgaagaaa ccaccgagcg
      721 cgaacgccac cagcacggag gccaggtaca tcaacccgat gcgcttgtga tcgagcgtga
      781 agatccagga caggagcccc ttgtcatgga ctaggtagtt gtcgtcggcg ctgcttcgtt
      841 gcgggatgac cgcgtcagcc gttgtactgg gtgatgccat gagaagcctc tgttcctact
      901 tcagggactt gatgtaggcg atgatgccgt cgagttcggc atccttgagt ttgccttggt
      961 acgtcggcat ggatggggtg aagcccgtca ccaccttcgc ggtgggatcc acgatcgact
     1021 cacgcaagta gttttcgtcg actttcaccg cgccggcgct ggtgttctcg gtcttgccga
     1081 acaggccctt ccaagtcgga ccgatcagcg gcgagccgtc gacggagtga caggtgttgc
     1141 agccttgctt ggcgtacaga ttggcgccga gctcctcggg cgtgcccttc cacaccgaag
     1201 catcgttgag ccacttctcg tacccgcccg gctcgtgcac gaccacctgg gtcagcatgt
     1261 tggagtgcga ggtgccgcag tactccgcgc aaaaaactgg gaactcgccg ggctggttcg
     1321 cctggaacca caggtccgta tagcggcccg gtacggcgtc catcttcgtg cggaaattcg
     1381 ggatgtatag gctgtgcagc acgtccaccg agttgatgag catccgcacc gggcgatcct
     1441 gcggcacgtg cagctgatcg tccacgtaac cgttggggta agtgaacagc cacttccact
     1501 tctgccccgt agcgcggatc tcgatggcgt tcttcggaac cgtgcgcagg tcgacgaacc
     1561 cgcggaagcc ccacacgaac agcgcaatca cgacgaacag cggcagcacc gaccacgtca
     1621 cctcgagtgc ggtgctgtga tcgctcgtgc gctccacttt ctggttcgcg ctgcgcgagc
     1681 gcgcccggta cttcacgatg aacaggatca tcgcgccgaa cacgagcacg cacgagatca
     1741 cactcatcca gtaaaggaag tagtagatcc agtcgacgct gcgcgtcgtc accgaagcgt
     1801 ccagcggcat ccagaaggta tcggactccg gcaccttcgg caggttcttc ggctgctcgc
     1861 cgacagtcag taagaggcta ctcaatcggg tcatgtggct gtgctctcca aaagctgccg
     1921 tttgcgcttg ccctcggatc ggcgcatcac gaataggaac cccgccaata ggacaacgct
     1981 gaccgcgccg gcgagcttca taatcttctg cgccaccggc gcatagcggc cttggctgga
     2041 gtcgtagtga aaacagtaga ggattagctt gtcgatcgtg ctgccaatct caccgcgcga
     2101 ggcctcggtg agggccaagc gcaaggtctg agtgtcgtac tgcagaccgt acaagtaacg
     2161 aacgatcgta ccatccggcg ccgcgagcac gatcgcggcg gggtgagcga actctttgcg
     2221 ctcgtcgacg taacggtacg agaagccaat cgcgtccgca taggctcgaa cattccccga
     2281 agaccccgag aggaacgccc acccgccgcg ggcttcgggc cgcccatact gcgtgagata
     2341 gcggctctcc gtgcgctgct gcagctcgag cggttcgttc ggctcgaggc tgaccgtaac
     2401 gactcggtag tcgcggttca cgccccaggc cacttgcttc aagctgttca cgagctggct
     2461 gagctgaagg ctgcagagca tcggacagtt cgagtaattc agcgtcagga ttacggggtg
     2521 cttgccgtcg aagtactggc cgagcacgac gtcgtttccg cgatggtcgc ggaagccgag
     2581 cgtctttggc aaaacctggc ccaagcgctc gttgacttcg acgccctgga gctgcttcgg
     2641 tacgggatcc gtgcgttcga cgcgttcgct ttccggcact ggcttcgccg caacggtgga
     2701 cgaaaccaga gtggtgctgg aaaccacgct gagcaggccg acgaccagtg tcgcccgcac
     2761 ggaagcagcc caagacgcca ttcaattagc catgcggttg tggagcggac ccgggctggg
     2821 ccgggggatt gggcgaaggt cgcgtcgggg cgagcggctg ggtcggttgt gaatgcgtcg
     2881 gcggaacggg ctgcgggctc accgctttcg gcccgggcgt gttcggggat ggtgccgcgc
     2941 cgcccgactc tttcgcattc ttggtgggag gggcgccatc caccgcgggc ggggtgccgc
     3001 cggtgatcgg agccgcgccg gtggcgcccg gctgcgagct cgggatcccg ccgggggctt
     3061 gagcaccaga gtccgccgga gagttagccg ctggagcagc actcgcgctg ccgggcggag
     3121 tcgcgctgct cggatcagcc ttcaaccgac tcaccacctg ctgcatcgac tgttcgatcg
     3181 gagtgccttg cccgagcttg tcgagttgag ccttcttgag cgctcggtac tcgtagctcg
     3241 cggattcacc gcgttcgccc gcggcttcgc ccaactcgcc gcgggtgagc gccgtgagcg
     3301 ccaccgcgac gcccagaaca gcgagcgtgc cgacgccaac cagcgtcatc agaacgcctg
     3361 tattgacctt gtcttcttcg gtagccatga cccgttcctc agatgttctc gaaagcgagc
     3421 gagcgcggca ggcgcgggtc cttgacgggc accaggttca cgttcttcgc cgcgaacgcg
     3481 agggccgcga cgacggcgcc ggccatgccg atccaggccg tgatgtccat gaggtggacg
     3541 tggagctctt ctttctgaac gttcggcatc accagccaat acatgtcgag atagatctcg
     3601 cacagcaccc aaaccgccca gaaagccagc gccttgcggt gccgcttcac gtggcgtgag
     3661 agcagcccga agaacggcac cacgaagtgc aggatcagga gagcccaaga aacgccgccc
     3721 caatcgccgg caaagcgctc cttgtaccag gctgtttctt cgggaatgtt cgcgtaccac
     3781 atcagcatga actgggagaa cccgatgtag gcccagaaca cggtgaacgc gaacatcatc
     3841 ttgcccaagt cgtggaagtg ctcgggggtg atgctcttgc tcaaacgtcc gcgcccctgc
     3901 agccacatca ggacgagaat gagcgtggcg tgcaccgcga gtacgcagct cgcgaagtag
     3961 tagacgccga agatcgtgct gaaccaatac ggatccagcg acatgatcag gtcgaaggcg
     4021 cagaaagtca gcgtcaacgc gaacgcgatc atgcccggcc ccgccacctt ctgcatgcgc
     4081 tcggcgatcg aagccgcgcc ggacttgtcc tggagcagtg actgcttgag gaagtaacgc
     4141 ccgagcagcg tccagaagcc gaagtagaac acgaagcgca ccaggaagaa gccagagttc
     4201 aagtacccgg ctttgtggtg cagcacgtgg tcccccgcga cggtctcgtg gttcgcccac
     4261 acgtacatga tgtcgttgcc gaggaagatc ggcacgacca cgggcagggc caagatcgcg
     4321 agcacgggga tgctcgtcgc gagtagctcg ccaacccggc gcagggccac gctccacttc
     4381 gcgttcacca ggttctgcag tgttacccag aacacggcgc ccaagccgat cgcgagcgcc
     4441 cacaggtagg ccgtcaagta cgagtaagag aagcgcttga agccatcgcc ctgcatcatg
     4501 ccgagcacga cggttgcgcc gagcccaacc gcagccgcgg cccccgcctt ggtgagcacg
     4561 ccgctcgcga gcgagcccgc gcggatattg tcaccgaagc ggtcggcagc ttcgtccgcg
     4621 gcgtctcgcg ccggcgcggc cttggaggtc gattctttgc ccgccggagc cttcgagctc
     4681 gggccctttg aatctttgga gtctttcgag gtcgccatga tgtctcggaa atcctattcg
     4741 ttccgcccag actcacttgt tcgggctgcg attctggaga gctcgcagat acatcacgat
     4801 ggcccaacga tcggccggct cgatttgcga agcataacca ggcatgtttc ggacgccgtt
     4861 gctgatcgtc tcgaacagct gtccgacggg ctgagctttg atgtagtcct ggttgacatt
     4921 gctgggttga acccaagcca tgcccttgcc acgaccgctg gccgcgagct cttccgcgcg
     4981 cttgtgaatc atgccgtcgc catcgccggc gaggccatgg caaggagcgc agtagatccc
     5041 gaagcgctcg cgaccgcgtg cgaccgtggc ctccgacgct ggcacctgtg cggggaatgc
     5101 ggttgcgtag gcgcctgcga gcttgccggt gtagaagtga tcgtccgcgc ggaactcgcc
     5161 gacggcaacc gtgcccggca cctgctcgcg cgtcgcccgt tcgtccgcga acgcgggatt
     5221 gcggcgctga gccttgtact tcggctgcca atccatatcg ccgatcacgt ggatgcgcgg
     5281 cgagcgtgag gtggagaagc gcgctttggc ggccatcgcg aacgggatcg ccgccgccgc
     5341 cgtcatgatg atcatgacgt aagcgatgaa cttgggcgga tcgttggagg tgacccggtc
     5401 ttccttcact tcgtcgagca ccacgggatg cgtggactcg agcaaagcgc gcgtcttgtg
     5461 accgtcgaac ttcggatcgc tcgcctggat catcaggaaa aagcggtcat ccgtcgcgcg
     5521 accgaatcgc tccttgagat cgagcgggtt cgagagctcg ggcagcccgt tcaggaccaa
     5581 catcgagacg acggtggtga tgcccgcgaa cagcaccatc agctcgaaca tgatgggtac
     5641 gttggcgggg atgctccaga acggcttacc gctgatgatc cacggataat cgacgccgtt
     5701 ggtccaatac tgcagggtcg ccgcaaagaa caacccgacc aggcccgctg ccaacgtcag
     5761 ccagggcagc accgtcatgc ggatgcccat cgcgttgtcg atgccgtgaa ttgggaacgg
     5821 agagtacgtg tcccaacgct cgaaacccgc gtcccgcacc ttcttggagg catcgaccag
     5881 atcccaaggt gactggtact cggcgagtag cccgtgcagc ggggcctcac caggtgagcc
     5941 ttcagtcttc ttgacttgcg cgtcgctcat cgtcgtatcc agaaatcagt ggcccgcgtg
     6001 tgcaggttgg ccagcgtgat cgtcgtgatg gtcgccgtga ccatgggcgt gcgccagcac
     6061 gctcgggtgg aaggctttga cctcggagat cgcaaccatc ggcaggtagc ggcagaacag
     6121 caggaacagg gtgaagaaga gcccgaagct gccgcccaag gtcatgaagt cgatcggagt
     6181 cgggatgtag taaccccagc tcgagggtag gaagtcacgg gtcagcgacg tgcagatgat
     6241 cacgaagcgc tcgaaccaca tgcccacgtt gatcagcatg ccgatcgcga gcgtcaccca
     6301 ggggttacgg cgcagcttct tcacccagaa gaactgcggc gcgatcacgt tgcaggtaat
     6361 catc
//
LOCUS       DASTKU010000292         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_57278,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000292 DASTKU010000000
VERSION     DASTKU010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57278"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFQ61_10530"
     CDS             <1..249
                     /locus_tag="VFQ61_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9954931.1"
                     /translation="CDGFVIQEHHLPEPVRVVTRSAPTEKPTLAMAVARLERQMIEEA
                     LRDARGNCARAARVLGTTERIVRYKAGKYGIDPGQYRE"
     gene            315..1133
                     /locus_tag="VFQ61_10535"
     CDS             315..1133
                     /locus_tag="VFQ61_10535"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET9954932.1"
                     /translation="MSRELSRLVEKHGGQPMSTPAVRECPELTPDIARQLVDDLASDR
                     HEIVVFMTGVAVSLLFEVAEQIGRRPDLVAALKRLTTVCRGPKPTAALRGFGVPPTLS
                     ARDPYTSAELIDAMSGIELAGRRVILLHYGERSETLAETLLARKANLEELWLYRWLMP
                     EDTDGLLSLVTRIVQGSVDALAITCQIQFRHLYAVAEQHGLHGKLVRALNDRVLVAAV
                     GPTCEAVLMAYGVEGAIMPDHPKMGPLVLTLMRHLEQRARRAEGAVEAVVQLTH"
     gene            1225..2259
                     /locus_tag="VFQ61_10540"
     CDS             1225..2259
                     /locus_tag="VFQ61_10540"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9954933.1"
                     /translation="MSVFGFGVIGTGQIARAFVAALAESQRCRVVHVLGSTPAKGHAF
                     AEQFGVARSAERVETLLEDPSVHGVYVAVPHPLHERFTSQALAHGKHVLCEKPLTLDS
                     RAAERLIAQARSARVILMEAFMYRCHPVIPALLDELQSGSIGMPRRLESDFCFEAPYD
                     PTGRLFCKELGGGAIWDVGGYPISLARLIAGTLEARPYAEPELVQVEGEVGPSGVDVF
                     ASAELRFASGFTARARCATRRALGNRSVIMGDAGQIEVPDLWLPGGERLGKTQGFVVR
                     GSVGDASARAVPPIRTVQVSAALAPFALEAERLLDAVELGEVPFPALDAGDTLGNLRT
                     LEAWLAALAH"
     gene            complement(2292..3083)
                     /locus_tag="VFQ61_10545"
     CDS             complement(2292..3083)
                     /locus_tag="VFQ61_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954934.1"
                     /translation="MKDQTVRCWGSNTSGQVGDGTYSSASNPVPVTGLAGITQLAVGR
                     DHRCALLNSGAVRCWGEGEFGQLGNGQLYTTSSTPVPVTGVSDAVQLAAGYQHTCALL
                     KNTTVTCWGWNGTGQIVGGSGNVATPVGQSLSRITQVVGTAYGTCAIQATTVQCWGMM
                     LRGGNAKLRQMSNLPSIAQLSAGQSHVCALSNAGTVYCWGENDFGQLGASEPATTEAP
                     IQILGLTDVARLVSGSTHTCAQLKDNSVKCWGGGYGASPTVVFDP"
BASE COUNT          538 a          934 c         1060 g          599 t
ORIGIN      
        1 tgcgacggct tcgtcattca agagcaccac ctcccagagc cggtgcgggt ggtgacgcgc
       61 agtgcgccta cggagaagcc cacgctcgcg atggcggtgg cgcgcctcga gcggcagatg
      121 atcgaagagg cgttgcggga cgcgcgcggc aactgcgcgc gcgcggcgcg agtgctgggc
      181 acgaccgagc gcatcgtgcg ctacaaggcc ggcaaatacg gcatcgatcc cggtcaatac
      241 cgagaatagg gaagccactc caaatgacga tcttcgacgc gcgtcggcca gtcgtagctc
      301 tgctcgaatc ccggatgagt cgcgaattat cgcggctcgt cgagaaacat ggcggccaac
      361 cgatgagcac gcccgccgtg cgtgagtgcc cggagctcac gccggacatc gcgcggcagt
      421 tggtcgacga cttggcttcg gatcgccacg agatcgtcgt gttcatgacg ggggtggcgg
      481 tctccttgct attcgaggtt gcggagcaaa ttgggcgccg tccagacttg gtggccgcgc
      541 tcaagcgcct aaccaccgtg tgccgtggtc ccaagccgac ggcggcgctg cgcggtttcg
      601 gggtgccacc gacgttgtct gcacgcgacc cgtacacatc ggccgagctg atcgacgcga
      661 tgagcggtat cgaactcgcc gggcgtcgcg tgatcctgct gcactacggt gagcgtagtg
      721 agaccttggc ggagacgctg ctcgcgcgaa aggcgaacct ggaagagctg tggctttatc
      781 gctggctgat gccggaagac accgatggct tgttgagcct ggtcacccgc atcgtgcaag
      841 gcagcgtgga tgcgctcgcc attacctgcc aaatccagtt ccgacacttg tatgcggtgg
      901 ccgagcagca cgggttgcac ggtaagctcg tacgcgcact gaacgaccga gtgctagtcg
      961 cggcggtagg cccgacttgc gaggcggtgc tgatggccta cggagtggag ggggccatca
     1021 tgcccgatca ccccaagatg gggcctctgg tgctcacgct gatgcgtcac ttggagcagc
     1081 gcgcgcgccg cgccgaaggt gcggtggaag ccgtggtgca gctcacgcat tgaggctcga
     1141 atttttgggg gccgcgcacg ccgctcggca atcctgctcc cggggcactt ggtgcagtcc
     1201 gggacaacgg ctatgtgttg accgatgtcg gtcttcggtt ttggcgtcat cggcacgggt
     1261 caaatcgcgc gggccttcgt cgccgcgctc gctgaatcgc aacgctgccg ggtggtgcat
     1321 gtgctcggga gtactccggc gaagggccac gccttcgcgg agcagttcgg ggtggcacgc
     1381 tcggcagagc gcgtggagac gttgctggag gatccgagcg ttcacggcgt gtacgtggcg
     1441 gtcccgcacc cgctccatga gcgcttcacg tcgcaggcgc tggcgcacgg caaacacgtg
     1501 ctttgtgaaa agccgctgac cttggactca cgagccgcgg agcgattgat cgcgcaggcg
     1561 cgctcagcgc gcgtgatcct aatggaagcc ttcatgtatc gctgccaccc ggtgatccct
     1621 gcgctgctag acgagttgca gtcgggtagc attgggatgc cgcgacggct cgagtcggat
     1681 ttctgcttcg aggcacccta cgatccgacg gggcgcctct tttgcaagga gctggggggc
     1741 ggtgcgatct gggatgtggg tggctaccca atttcgttgg cgcgcttgat cgcgggcacg
     1801 ctcgaggcac gaccctatgc ggagccagag ctggtccaag tggaaggaga agtgggcccc
     1861 tccggcgtcg atgtgttcgc ttcggcggag ctgcggtttg cgtcggggtt tacggcgcga
     1921 gcgcgctgcg ccacgcgacg cgcgctcggt aatcgcagcg tgatcatggg ggacgcgggg
     1981 cagatcgaag taccggatct ttggctgccg ggtggtgaac ggctgggcaa aacgcaaggc
     2041 ttcgtggttc ggggctcggt gggtgacgcg agcgcgcggg ccgttccgcc gattcgaacg
     2101 gtccaagtga gcgcggcact tgctccgttc gcgctcgagg ccgaacgact gctggatgcg
     2161 gtagagctcg gggaggtgcc tttccctgcc ttggatgccg gcgacacgct gggaaatttg
     2221 cggacattgg aggcatggct cgcggctctg gcacattagc gattgcgtgg ccacgcaagt
     2281 gatggcgctc gttacggatc gaagacgacg gttggcgaag ctccatagcc gccgccccaa
     2341 catttgacgg agttgtcttt gagctgagcg caggtgtggg ttgacccgga aacgagtcgc
     2401 gcgacatcag tcaatccgag aatctgaatc ggggcctcag tggtcgcggg ctcggatgcg
     2461 cctagctgac cgaagtcgtt ctctccccag cagtacacgg tgccagcgtt gctcaatgca
     2521 catacatggg actgaccggc ggacagttgc gcgatagagg gcaagttgga catttgccgc
     2581 aacttcgcgt tgcctcccct aagcatcatg ccccagcact ggacggttgt ggcttgaatt
     2641 gcacacgttc cataggcggt gccgacaacc tgcgtgattc gcgaaaggct ttgaccgaca
     2701 ggcgttgcga cgtttccaga gccgccgaca atttgacctg ttccgttcca gccccaacac
     2761 gttacggtcg tgttcttgag cagagcgcag gtgtgctggt agcccgccgc caactggact
     2821 gcgtcgctca cccccgtcac tggcactggc gtcgaggaag ttgtgtacag ctgaccgttt
     2881 ccgagttggc caaactcacc ttctccccaa catctgactg cacccgaatt gagcagggca
     2941 caccgatgat ctctgccgac tgccagttga gtaatgcctg ctaacccggt gactggcacc
     3001 gggttgcttg cgcttgagta cgtcccgtcc ccgacctgac cgctagtgtt gctcccccag
     3061 catctgaccg tctgatcttt catgatcacg caaggcgagg caatttccag aacactgagc
     3121 cccgcgagct g
//
LOCUS       DASTKU010000293        10250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_57354,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000293 DASTKU010000000
VERSION     DASTKU010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10250
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57354"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFQ61_10550"
     CDS             complement(<1..618)
                     /locus_tag="VFQ61_10550"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HET9954935.1"
                     /translation="MSEQGGSPEGGSGPLTPRVEGDASGATALPSDPSPDNVTASKPM
                     DATRADVAEAPVKGSASASEPKAAQPKLVSGSGRPEEDTARAGEQRFFVRVLMRERNA
                     GAIYDAAKETSTPNHLQYGRFLPHERIQAFGNPDPHRLRALIQLFRSAGLEVHELENS
                     PLVMAVGNRLTLSTFFESDALERVLQKAARGTLVPPWEWSDQHLRD"
     gene            740..1456
                     /locus_tag="VFQ61_10555"
     CDS             740..1456
                     /locus_tag="VFQ61_10555"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HET9954936.1"
                     /translation="MYQKPHQKSERAIPKEYRFVRLPLPEAAHSALEAARREAADSDL
                     EFVASQWKWHVETRFHVLRGGPTRGFPGSELVSGSGVDHPNLQRLPSIRRFLELAFPE
                     PPVLAWLGDSPPGARIFLHVDNTKHWDEHHRIHVPLLTHGEARLCVDGFFQHLEYGSA
                     WLLNNSVPHGAINRGPRRVHLMLDLRASEWMADWLRRGTSESGTPDSLALAELGRDPL
                     SALSSGDCFDSARLSRLISQ"
     gene            1558..2997
                     /locus_tag="VFQ61_10560"
     CDS             1558..2997
                     /locus_tag="VFQ61_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET9954937.1"
                     /translation="MAELRRAPFAPNSRYAIDADICRRLLTIALSRGGQYADLFFEYR
                     VGAGITFEEGITRSASRGVSMGLGVRVLMGDATGFAYVEDLAWEAMQRAAETAAQIAS
                     GGKSVPAVGVQPVVTAERYLLDAPSIDVSGMEKRRLLERASQKAHQLDSKIVKVEASF
                     SEELREILIATSDGRMAYDVQPLMRFGVRAVAEREGRRESGSSGGGGRMSLAYFSDKS
                     PEYHAEKAVHQALVLLDARPAPAGQMEVVLAPGDSGILLHEAVGHGLEADFNRKGTSN
                     YTGQVGNVVASPLCTVVDDPTLLMSRGTINIDDEGNEPTRSVLIEDGLLRGYMQDRMS
                     AHHFAVNPTGNGRRQAFNCVPMPRMTNTVLLAGPHDPAEIVRSVKRGILAKSFGGGQV
                     DISNGDFVFSLTESYLIEDGQVTAPLKGVNLIGNGPETLREVTMLGNDVAVSDGIWTC
                     GKDGQSVPVGVGCPTIKIARITVGGTEIS"
     gene            3050..3826
                     /locus_tag="VFQ61_10565"
     CDS             3050..3826
                     /locus_tag="VFQ61_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9954938.1"
                     /translation="MLDREDGASSTLRQLGATVRTLDLWDDPVNLISDDDDDLGIRPR
                     ALVFEALDRPDLAIAALRAVRKEPYFDNVGALISVTVGQIARIDPSSGFDDFVLHPYV
                     PEELYGRIRALEWRRSEFATEERHKVAGIVVDKAGHEVYVDGKPVQLTAKEFALLAYL
                     CERRGKVLSREHLLARVWGNRYEGGPRTVDIHVRRLRAKLGAALPLETLRGSGYKLRA
                     PDLEEKIERVSVENAGPAETDASEPESGPDEEDEGDDAEE"
     gene            3947..5341
                     /locus_tag="VFQ61_10570"
     CDS             3947..5341
                     /locus_tag="VFQ61_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954939.1"
                     /translation="MQPLRALLRCASVLGFGLASSMASGREPKQPDAETRDTYVLRAH
                     GETHALLFQRALLPGSRGALVSTETLLPVYQYWVLSARDIDATWQRDSLDVDVALWGR
                     AALFDNDREGRLNGDVQSANVRYRHGPAALRLGRQHVAGGAARYARFDGANLELSLGN
                     ELSLSAYAGFAVMPRWNERPGYQYLGAVTDSEIRELARTPLASRDAQWLTGGRVAWAR
                     RDIGLGLTWHERHETGGVSHRSLGLDTRARIGAFGVGGNAILELESSRFADASLFVDY
                     APLRNIDVSLEYLHTEPALLLSRQSVLSVFSRETYEETGGFTRYRPTPELLLEAGGFV
                     QWFASGRPGRRAEFIASSWLDAERRSLLRANYTRVITPDNAYWSLRGSLLRQLGTGFR
                     ATVDAYLYLYDHAIEGYRSATVYAGTLSYQPLRELNVLWGASLARTPLAGLDAQTQLR
                     VACDFDWVTHGGRR"
     gene            5338..6411
                     /locus_tag="VFQ61_10575"
     CDS             5338..6411
                     /locus_tag="VFQ61_10575"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET9954940.1"
                     /translation="MKDELRGAHWLWSVVLIVAIVGACVRARTARFPHRPHVAEGTCQ
                     TQCLTCTSCHAHATEGKPTGGTAGVKGALAHAALPEASLCETCHHDEPKKLATRVAPP
                     TPDETPIRFDHDRHLAMPGIAGQCVGCHAGVVESGKPNLPPMTQCFSCHEHEQQWQRA
                     QCAPCHADRELRSLIPRSFLPHDQAFARRHGQAANQQMSLCRSCHAPADCEACHDTAQ
                     ELPIERRLPEKVERRFVHRGDYLTVHGIEASSQPSRCLRCHEVQTCDSCHSARGVSAG
                     LMAGRNPHPPGWVGANTQSFGFHGREARRNILSCAGCHDQGPATNCIRCHRVGGYGGN
                     PHPAGRWPGADPASETMCRYCHE"
     gene            6404..8131
                     /locus_tag="VFQ61_10580"
     CDS             6404..8131
                     /locus_tag="VFQ61_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxxxCH/CxxCH domain-containing protein"
                     /protein_id="HET9954941.1"
                     /translation="MNDRLPAILASLGSWLTALTSCLALWGSLLCGSCLERRASVEDT
                     SEVRRCAQCHGDPKRVGDYLERSAPPNDLRSAAASDSPGVGAHSIHLRDSETHSALAC
                     TECHVVPTRVEDVGHADTPLPAELNFGPLASKDGRQPTYDPASRTCSNTWCHRSADAV
                     WTAPHDSEQACGSCHGLPPEKPHPQSERCFACHAEVIDEARHIVAPKLHVDGIVQYQA
                     GPCSSCHGRGKASAPPLDTQGNSSPSSRGVGAHEAHLSGGSNARPLECSECHHVPERI
                     DEPTHADGLPAEVSLIGVAQSHDFSARWQPSEARCSDTWCHGPTATTSRSSPRWDQPQ
                     TLDCTGCHGMPPAAPHPAATQCGFCHSAVVSSDHLTIHDRNRHVDGTVDVAVGNSCTG
                     CHGSTDAASAIQAAPPRDLMGGTEPSSRGVGAHSIHLNGTENSRPVPCETCHVVPTRV
                     LAEGHLDGEPPDVVFSGLASAFGAAPKYIEGRCADTACHGAFFPEGHRSGGSRTTPEW
                     TNPRSSEAACGSCHSLPPPRPHPYAENSPDCSSCHENVAPDNRSFVRPDLHVDGVVTF
                     TLPQNSSGP"
     gene            complement(8146..10053)
                     /locus_tag="VFQ61_10585"
     CDS             complement(8146..10053)
                     /locus_tag="VFQ61_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954942.1"
                     /translation="MWLVLAALLAAAYACSDRETNREPAPPSDGKTTGAYVSVPESWQ
                     LARSIPGHRGHVIEKKVPCVRCHSLTKDGMGPVTPDRCASCHEREAQLQHAVELAHGR
                     FGPSAGSDCQSCHAFSVEDAREVHASSSPLPASNQGSAPAGKQLQAAAPTDCIRCHAQ
                     PLGHTPAIRVHETSECIKCHQVHLEPRPMATACPECHSKVHTRHAAQGHSDVQVCTAC
                     HTNPHEPAERAKATCQECHSKQSPRIPATALFATTTALDMPGVGTVSRTPSAAAASGA
                     PSNTGRGHTACTDCHRAHEFGADQAANCRTCHSSLPVMGAAQNPAHQRCINCHSPHNV
                     RGTPQGACASCHKQVHSDHPTRAGAPCTGCHDPHPQQAAVGAASSGASHPETARPCSS
                     CHQSAPSDRDFHQGVSCRECHRPHEFLANAKAACSECHLSQVTHTASAAGHADCQGCH
                     RGLPHAPAVARSACDACHRYEARQVHAGHENCRQCHEPHSGAVETACSTCHGAESASA
                     PPGHQKCTGCHDQHAALPVKASCGSCHRQEQQSAHGKIASGCGSCHRPHGPTGPSQVP
                     ACTSCHQISNLGGLHEVAKHAGSCTSCHSGHGARDAVGRATCLGCHSDRTQHFPDANR
                     CTSCHLFGARR"
BASE COUNT         1822 a         3255 c         3330 g         1843 t
ORIGIN      
        1 gtcgcgcagg tgttgatccg accactccca cggcggaacc aacgtgccgc gcgcggcttt
       61 ctgcaacact cgctccagcg cgtccgattc gaaaaaagtg gagagcgtga gacggttgcc
      121 caccgccatc accagcggtg aattctcgag ttcatgcacc tcgagcccgg ccgatctgaa
      181 gagttggatg agtgcgcgca gccgatgcgg gtcgggattg ccgaacgcct gaattcgctc
      241 gtgcgggaga aacctcccat actgcaagtg gttgggcgtc gaggtctcct tggccgcgtc
      301 gtaaatcgcg cccgcgttac gctcgcgcat cagaacgcgc acgaagaagc gctgctcgcc
      361 cgcgcgggcc gtgtcttctt cgggccgccc cgaaccactg actagcttgg gctgcgcggc
      421 cttcggttcc gacgccgacg cggacccttt cacaggcgcc tcggcgacat ccgcgcgggt
      481 cgcatccata ggcttcgaag ccgtcacatt gtctggcgaa ggatcgcttg gcaaagccgt
      541 ggccccgctc gcatcgcctt cgactcgtgg ggtaaggggc cccgagccgc cctccggact
      601 gccgccctgt tcgctcattg gtcctcgtcc tccaaagctt ggtcgcacct gcaccgccgg
      661 ctatgatggt gctcgaatgc tcctcgatgc tcttcgatgc ttcctcgtat gccgcacgta
      721 ggcgcgagat actcgccgaa tgtaccagaa gccgcatcaa aagagtgaac gcgcaattcc
      781 gaaagaatat cggttcgtgc gcttgccctt gcccgaagcg gcacattcgg ccctcgaagc
      841 ggcgcgccga gaggccgccg acagcgactt ggagttcgtc gcgagccaat ggaaatggca
      901 cgtggaaact cggttccacg tgctccgcgg gggaccgaca cgcggctttc ccggtagcga
      961 gttggtgtcc ggctcgggcg ttgaccatcc caacctgcag cgtctcccct cgatccggcg
     1021 ctttctcgag ctggcttttc ccgaaccccc tgtgctcgct tggctgggcg acagcccgcc
     1081 cggagctcgc atcttcctgc acgtcgacaa caccaagcac tgggacgagc atcaccggat
     1141 tcatgtccct ctgctcacgc acggcgaggc gcgtttgtgc gtcgacgggt tcttccaaca
     1201 cctcgagtat ggaagtgctt ggttgctgaa caacagcgtc ccgcacgggg caatcaatcg
     1261 ggggcctcgt cgcgtgcact tgatgctcga cctccgcgcg agcgaatgga tggctgactg
     1321 gctgcggcga ggtacttcgg agtccggaac gcctgattca ctcgcgttgg ccgagctcgg
     1381 gcgcgatccg ttgagcgcgt tgagctccgg tgattgcttc gatagcgcgc gcttatcgcg
     1441 cttgatttcg cagtagccac cgccgtcacc atccccattc ggggccgcca tgttggattg
     1501 acctctgccc atgcgggtac cttgacgagg ctcctgatcg gccgaagact gccgttcatg
     1561 gccgaattgc gccgcgctcc gtttgctccg aactctcgtt acgccattga cgccgacatt
     1621 tgccgtcgcc tgctgaccat cgcgttgtcg cgaggcgggc agtacgccga tttgttcttc
     1681 gaataccgcg tgggcgcggg catcaccttc gaggagggga taactcgcag cgcttcgcgc
     1741 ggtgtgagca tgggcctggg cgttcgcgtg ctgatggggg acgcgaccgg cttcgcttac
     1801 gtggaagatc tggcgtggga agccatgcaa cgtgccgccg agaccgccgc acagatcgcg
     1861 agcggtggaa agagcgtgcc ggccgtaggc gtgcagcccg tcgtcaccgc ggagcgatac
     1921 ctgctcgatg cgccgagcat cgatgtctcg ggcatggaga agcggcgcct gctcgagcgc
     1981 gcgagtcaga aggcgcatca gctcgactcg aagatcgtca aggtcgaggc aagcttctcg
     2041 gaagaactac gtgagatctt gattgccacg agcgatggtc gcatggcgta cgacgtgcag
     2101 ccgttgatgc gtttcggcgt ccgcgccgtg gccgagcgcg aggggcgtcg agagtcaggc
     2161 agcagcggcg gcggcggccg catgtcgtta gcctactttt ccgacaaatc acccgagtac
     2221 cacgcggaaa aggccgtgca tcaagccctg gtgttgctcg atgcgcggcc agcccccgcg
     2281 gggcagatgg aagtggtgtt ggcgcctggt gatagcggca tcctgttaca cgaagccgtg
     2341 ggccacgggc tcgaagccga cttcaaccgc aaaggcacca gcaactacac cgggcaggta
     2401 gggaacgtcg ttgcaagccc gctctgcacg gtggtcgatg atccgactct gctcatgagt
     2461 cgaggcacga tcaacatcga tgacgaaggc aacgaaccca cgcgcagcgt gctgatcgag
     2521 gacggcttgc tgcgcggcta catgcaggat cgcatgtcgg cgcatcactt cgctgtgaac
     2581 cctacgggta atgggcggcg acaggcattc aactgcgtac ccatgccccg aatgaccaac
     2641 accgtcctcc tggccggtcc gcatgatcca gctgaaatcg tgcgcagtgt gaagcgcgga
     2701 atcctggcta agtccttcgg tggtggccag gtggacatct cgaatggcga cttcgttttc
     2761 tctctgacgg agagctactt gatcgaagat ggacaggtca cggcacctct gaaaggtgtc
     2821 aacttgattg gtaacggtcc cgaaacactg cgcgaggtaa cgatgttggg aaatgacgtg
     2881 gctgtttcag acggaatttg gacgtgcggc aaagacggcc aaagcgtccc cgtcggagtc
     2941 ggctgcccaa ccatcaaaat tgctcggata accgtcggtg gcactgaaat ctcctgattt
     3001 tacgacgcgc aaagccaaaa gcttcgttgc tgtggttggg catggtccca tgctcgaccg
     3061 cgaagacgga gcgtcttcga cgttgcgcca actaggtgca acggttcgca cactcgactt
     3121 gtgggatgac ccggtcaatt tgatctccga tgacgatgac gacctgggga ttcggccgcg
     3181 tgcgctagtt ttcgaagcgc tcgatcgacc ggatctggcc atcgcggcgc ttcgcgccgt
     3241 gagaaaggag ccctacttcg acaacgtagg cgccttgatt tccgtcacgg tcgggcagat
     3301 tgctcgtatc gacccatcga gtggcttcga tgacttcgtg ttgcaccctt acgttccgga
     3361 ggagctgtat gggcgcattc gtgctctcga gtggcggcgc agcgagttcg ccaccgagga
     3421 acgccacaag gtggctggca tcgtcgtcga caaggcgggc cacgaagttt acgtcgacgg
     3481 caaacccgtg cagctcacgg ccaaggagtt cgccctacta gcttacctct gcgagcgtcg
     3541 cggcaaggtc ctctcccgtg agcacctgct agcccgcgtg tggggaaacc gctacgaagg
     3601 tgggcctagg accgtcgaca tccacgtgcg caggttacgc gcaaagttgg gcgcggcgct
     3661 cccgctcgaa acactgcgcg gcagtggata caagctgcgg gcccccgatc tcgaggagaa
     3721 gatcgagcgg gtgagcgtcg agaatgccgg tcccgcggag acggacgcga gtgagccgga
     3781 gtccggcccg gacgaagagg acgaaggcga cgacgcggag gagtaggcct ggaatctgtc
     3841 agctttcgtc aggctgacag attccgcccg tcacacagga tattgtgtgt gaccgcgcga
     3901 atcgtttcga tcgcgagccg gtcgcgttag aagtgggggt tgcccgatgc aacccctcag
     3961 agcactcctc cgctgtgcat ccgtgctcgg cttcgggctg gcgagcagca tggcttcggg
     4021 gcgcgaaccc aagcagcccg atgcagagac acgcgacacg tacgtgttgc gtgcgcacgg
     4081 cgagacgcac gcgctccttt ttcaacgcgc actcttgccc ggcagtagag gcgcgctcgt
     4141 ttccactgaa acgctgctgc cggtctacca gtattgggtg ctgtcggcgc gagacatcga
     4201 cgcaacctgg cagcgcgaca gcctcgacgt cgacgtagcg ctgtggggcc gagccgcgct
     4261 gttcgacaat gatcgcgaag ggcgcctgaa cggggatgtt cagagcgcga atgttcgcta
     4321 ccggcatggc ccggccgcgc tgcgtctcgg gcgacaacac gttgcggggg gcgccgcgcg
     4381 ctacgcgcga ttcgacggtg cgaacctgga actgtcacta ggcaacgagc tctcgctcag
     4441 cgcctatgcc gggttcgcgg tcatgccacg ctggaacgag cgacctgggt atcaatatct
     4501 aggagcggtc accgactccg aaataaggga gctggcgcgc acaccactag cgagccgaga
     4561 cgcgcaatgg ttgaccggcg ggcgcgtcgc gtgggctcgt cgcgatatcg gtttgggcct
     4621 gacttggcac gaacgacacg agaccggtgg cgtatcccac cgaagtctcg ggctcgatac
     4681 gagagctcga atcggagcct tcggggtggg cggcaacgcg atcctggaac tcgagtcgag
     4741 tcgtttcgcc gacgcgagtc tgttcgtgga ctacgctcca cttcgaaaca tcgacgtctc
     4801 gctcgagtat ctgcacaccg agccggcgct gctgctctcg cgtcaatccg tactctcggt
     4861 attcagccgc gagacttacg aggagaccgg cggcttcacc cgatatcggc caaccccaga
     4921 gctgctgtta gaggccggcg gcttcgtaca atggtttgcg tcgggccgcc ctgggcggcg
     4981 tgccgagttc atcgccagta gctggctcga cgcggagagg cgctctttgc tacgcgcgaa
     5041 ctatacgcgc gtgatcacgc cggacaacgc ttattggtcc ctgcgcggct ccctgctgag
     5101 gcagctcggg acgggctttc gagccacggt ggacgcttat ttgtacctct acgaccacgc
     5161 gatcgaaggc tatcggagcg cgaccgttta cgcgggcacc ctctcttatc agcctttgcg
     5221 agagctgaac gtactatggg gggcttcgct ggcacgtact ccgttagccg ggctagacgc
     5281 gcagacgcag ctccgcgttg cctgtgattt cgactgggta acgcacggag ggcgccggtg
     5341 aaagacgagc ttcgaggcgc acattggctg tggagcgtcg tgctgatcgt ggcgatagtc
     5401 ggagcctgcg ttcgcgcgcg aactgcacgt ttcccgcacc gaccgcacgt agcggaggga
     5461 acttgccaga cccagtgttt gacctgcacg agctgtcacg ctcacgcgac cgagggcaag
     5521 ccgactgggg gaaccgccgg ggtgaagggt gcgcttgcgc acgcagccct tcccgaagcg
     5581 agcctgtgcg aaacctgtca ccacgacgag cccaagaaac tcgccaccag agtcgcgccc
     5641 ccgacgcctg acgagacccc aatccgtttc gaccacgacc ggcatttggc catgccgggc
     5701 atcgcggggc agtgcgtggg ctgtcacgcc ggcgtggtgg agagcggcaa gccgaatcta
     5761 ccgcccatga cccagtgctt cagctgccac gagcacgagc agcagtggca acgcgcgcaa
     5821 tgcgcccctt gccatgccga tcgcgagttg cggagcttga tcccgcgcag ttttctgccc
     5881 cacgatcagg ctttcgcgcg tcgtcacgga caggcggcga atcaacagat gtcgctgtgt
     5941 aggagctgcc acgcgccggc cgactgcgag gcctgccatg acacggcgca agagctgccc
     6001 atcgagcgtc gactgccgga gaaggtggaa cgtcgtttcg tacatcgcgg agactacctg
     6061 accgtgcatg ggatcgaggc gagctcccaa cccagccgct gcctgcgttg ccacgaagtg
     6121 cagacctgcg atagctgcca cagcgctcgt ggcgttagcg cagggctaat ggcagggcgc
     6181 aacccgcacc caccaggttg ggtcggcgcg aatacccagt cctttggttt ccacggccgc
     6241 gaagcgcggc gaaacatctt gagttgtgcg ggctgtcacg atcaggggcc cgcaaccaac
     6301 tgcattcgtt gtcaccgggt cggcggctac ggcggcaacc cgcaccccgc cggtcgttgg
     6361 ccaggagcgg accccgcgag cgaaaccatg tgccggtatt gccatgaatg atcgattgcc
     6421 cgcgatcctc gcgagtctag gcagctggct tacggcgctt acatcgtgcc tcgcactgtg
     6481 ggggagttta ctgtgcggca gctgcctcga gcgccgcgcc tccgtggagg acacgtcgga
     6541 agtgaggcgc tgcgcccaat gccacggcga tccgaagcga gtcggggatt acttggagcg
     6601 ctccgccccc ccaaacgatc tcagaagtgc ggctgcgagt gacagcccag gtgtcggcgc
     6661 gcactcgatt cacctgcggg attcagagac acactcggca cttgcttgca ccgagtgtca
     6721 cgtggtcccc acgcgagtcg aggatgtcgg gcatgccgat acgccgctgc cggccgagct
     6781 gaacttcggc ccgctcgcga gcaaagacgg gcgccaaccc acttatgatc ccgcctcccg
     6841 gacttgcagc aatacctggt gccatcgctc ggcggacgcg gtctggacgg ctcctcacga
     6901 ttcagagcaa gcctgtggtt cgtgccacgg tttgcccccg gagaagcctc acccgcaatc
     6961 cgagcgttgc ttcgcgtgtc atgcggaagt gattgacgag gcacgccaca tcgtagcgcc
     7021 gaagctgcac gtggacggca tcgtgcagta tcaagcaggg ccgtgttcga gttgccatgg
     7081 gcgaggcaaa gcctcggctc cccccctcga tacgcagggc aactcgtccc cctcgtcacg
     7141 cggcgttgga gcacacgagg ctcacttgtc aggcggtagt aatgctcgac ctctcgaatg
     7201 cagcgagtgc caccacgtgc cagagcgaat cgacgagccc acccatgcgg atggtttacc
     7261 cgcggaagta agcctaatcg gcgtggccca aagccatgac ttcagcgcgc gctggcaacc
     7321 gagcgaggcg cgctgcagcg atacttggtg tcatggaccg accgcaacca cgagccgctc
     7381 gtcgccacgc tgggatcagc cgcaaacgct ggactgcact ggctgtcacg gcatgcctcc
     7441 tgcagctccg caccccgccg cgacccaatg cgggttctgt cactcggccg tcgtctcgtc
     7501 cgaccacctc acgattcacg accgcaatcg gcacgtggac ggcacggtcg atgtcgcagt
     7561 gggaaactcc tgcaccggtt gccacggctc caccgacgcc gcgagcgcga ttcaagccgc
     7621 tccgccgcgc gatctgatgg gcggtacgga gccatcgtct cgcggcgtgg gcgcacacag
     7681 tatccatctg aacggaacgg agaattcgcg cccagtaccg tgcgagacgt gccacgtcgt
     7741 gcccactcgg gtgctggcgg aggggcatct cgacggcgag ccgccggacg tcgtgttctc
     7801 cgggctcgcc agcgcgttcg gagccgcgcc caagtacatc gagggtcgtt gcgcggatac
     7861 ggcttgccac ggcgcatttt tcccggaagg ccatcgctcg ggtggcagtc gaaccactcc
     7921 ggagtggacc aacccccgca gcagcgaagc ggcgtgcgga agctgccaca gtctgcctcc
     7981 gccccggccg cacccttatg cggagaactc acccgactgc agttcctgcc acgagaacgt
     8041 cgctccggac aatcgaagct tcgtacgccc agacctccac gtagatggtg tggtcacatt
     8101 cacgcttccg caaaactcga gcggcccctg aagcaagtgc gcagttcagc gccgcgcacc
     8161 gaacagatgg cagctcgtgc agcgattggc atccggaaag tgctgagtgc ggtcgctgtg
     8221 acagcccagg caagtcgccc tacccactgc gtcgcgcgcg ccgtgcccag aatgacagct
     8281 cgtgcacgag cccgcatgct tggccacttc gtggagcccg ccgagattgg agatctgatg
     8341 acagctcgtg caggccggta cctgcgaggg accggtgggg ccgtgcggtc gatgacaact
     8401 tccgcagccg ctcgcgatct tcccatgagc ggactgttgc tcctgtcggt ggcagctgcc
     8461 gcagctagcc ttgactggta gcgccgcgtg ctggtcgtga catccagtgc acttctggtg
     8521 gcccgggggt gcgctcgcgc tctcggcgcc gtggcaagtc gagcacgccg tctcgaccgc
     8581 gccgctgtgg ggctcgtggc actggcggca gttctcgtgg cccgcgtgca cctgacgggc
     8641 ctcgtaccga tggcacgcat cgcacgctga ccgggctacc gcaggagcgt gcggtagacc
     8701 ccgatggcac ccctggcagt cggcatgtcc ggccgcgctg gccgtgtgtg tgacttgtga
     8761 aaggtggcac tcggaacacg ccgccttggc attcgccaga aactcgtgcg ggcggtggca
     8821 ctctcggcac gaaacccctt gatgaaagtc tcgatctgaa ggcgccgatt ggtgacacga
     8881 actacaaggc cgggcggtct ctgggtgcga ggcgccgctc gaggccgcgc ccaccgccgc
     8941 ttgctgtgga tgagggtcat gacagcccgt gcacggtgca ccagcgcgcg tggggtgatc
     9001 actatggact tgcttgtgac aactcgcgca cgccccctgc ggagtgccgc ggacgttgtg
     9061 tggagagtgg cagttgatgc agcgctgatg ggctgggttc tgggcggcgc ccatgacggg
     9121 caaactggag tgacacgtac gacagttggc agcctgatcg gcgccgaact catgagcgcg
     9181 atgacagtcg gtgcacgcgg tatgccctcg gcccgtgttc gatggagcgc ccgaagcggc
     9241 cgcggccgag ggggttcgag agacggttcc tacaccgggc atgtccagcg ccgtcgttgt
     9301 cgcgaataag gcggttgcgg gaatgcgcgg cgattgcttg gagtggcact cctggcaggt
     9361 cgctttggcg cgttctgccg gttcgtgagg attggtgtgg cacgcggtac aaacctgcac
     9421 gtcggaatgc ccctgcgccg cgtgtcgagt gtgaaccttc gaatggcact cgggacaagc
     9481 ggtggccatg gggcgaggct cgaggtgcac ctggtggcac ttgatgcact cgctcgtttc
     9541 gtgcactcgg atggcaggag tgtgccccag gggctgggcg tgacaacgaa tgcagtcggt
     9601 cggcgcggcc gcttgaagct gcttgcctgc cggggcggag ccttgattac tcgccggcaa
     9661 aggcgatgag gaggcgtgca cctcccgcgc gtcttccacg ctgaaagcgt ggcagctctg
     9721 gcaatcgctc cccgctgaag gcccgaagcg cccatgggca agctcgaccg cgtgctggag
     9781 ctgagcctcg cgctcgtggc aactggcaca gcgatcggga gtcacggggc ccatgccgtc
     9841 cttcgtcagg ctatggcagc gcacacacgg caccttcttc tcaatcacat ggccccggtg
     9901 ccccgggata ctgcgagcaa gctgccaaga ctcgggcacg gatacatacg cccccgttgt
     9961 cttgccatcc gacggcggag cgggctcccg attggtctct cgatcgctgc acgcgtaagc
    10021 ggcggcgagc agcgcagcaa ggacgagcca caccgaatgc actggaggat gcgcttcgac
    10081 caacagaatc caccaataga aacactcagc atatccaaca cgccgcgtcc tagtcgaccg
    10141 ctttccgccc ccgagttgga gaaacggtga ttacggccgc ggtctcggcc cgaacgtttt
    10201 tcgaggggcg gaatcggctc gtgtcccgcc ttgtcggtgt tcaaggcacc
//
LOCUS       DASTKU010000294         9121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_503514, whole genome shotgun sequence.
ACCESSION   DASTKU010000294 DASTKU010000000
VERSION     DASTKU010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503514"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..968
                     /locus_tag="VFQ61_10590"
     CDS             192..968
                     /locus_tag="VFQ61_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954943.1"
                     /translation="MIAEGAPLTWVQHANDLEAAERWGATCRTVGADHNETIVAILKW
                     PNDCKDLARRLGGRWPIVERFDDPDLLKLGAKLADADGPTVVAELVDFVSERMTGVGT
                     ALKTAVEAIKAGRGTSRITKNRRHADRLSALASAPTPARALEWLEGVLAHREDWWLYR
                     RECVFQLREALRHCSGATLAELPDMVAAARTRARHRGRYTHRRTIGTPLLVKGLEFDH
                     AVLLWEPNHLSAQGLYVALTRASKSLTIVSRTRTLIPACA"
     gene            complement(1162..2343)
                     /locus_tag="VFQ61_10595"
     CDS             complement(1162..2343)
                     /locus_tag="VFQ61_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954944.1"
                     /translation="MVRRIVESIQHERDFKVLCDGLLRFEFGHLVQTFASAGADRGVD
                     AEYHGLVDGVRGRWLFQYKFRSPIEGVSRRRSWLGGCYLRSGERKGEFDKPGVAEADG
                     YLLLTNIPVTVGLVGQLRDAWLSRKANAPFCVWDPSRLNVLLKGREHLARSWTGAREA
                     HCQRAIVGPLWAWIEDALRVTSDWSSDPLWPLAIEDHDQRVPTAAFHQSFVVRHGVKI
                     QPRVAELRVATRDPLFRYAASIAFPNALAPLQAVDGAVKLLCDSIRQEIVGVQRELGR
                     RLPKLSQLREHDPQEVALALAYCVLEVAWGFPGRGFHSVKSGRFIANGTKIVCEDPAL
                     ADAEPVLDELVGALERGVVPETVATARTAVEGIIFDWAERMYEVVTFGLDAQSSTGET
                     D"
     gene            complement(2802..4154)
                     /locus_tag="VFQ61_10600"
     CDS             complement(2802..4154)
                     /locus_tag="VFQ61_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET9954945.1"
                     /translation="MADKIPAKVSGGRPATGSIVWEDPETKTRPRGVRVTRADGRRRV
                     IRFDPGTSPEEALTLAPVMAERARLAVDASAGETVAEYAARWCSWREARGLSSAKLDG
                     ARLGCHVFPILGVLGMRDVTRDDVRRLVSALDNKARVGFYNETGGKRRPFAWKTAVNV
                     WSIARAMFRDACSAKRVDLRVRDDNPADGVPGPDIGAHKAKSYLWPSEFTALVSSEAV
                     PLAWRRMFAVTTYLYARAGEVNALRWEDVDLERAVVHVHASVNRDTGTRKATKTGDAR
                     RVPIERELIPLLRAMHDETGGRGVVAPVRATDRKLSRQLRRCLALAGVRRAELFVMGD
                     PTRKAITFHDLRATGITWCAVRGDDPLKVKQRAGHRTFSTTEGYIREAENLRDGFGEV
                     FPPLPPSLIVSTKPKNRRRVSASVSAFGSVPVALLAKNRRDLVEAPGIEPGGAICRLA
                     "
     gene            complement(4887..5630)
                     /locus_tag="VFQ61_10605"
     CDS             complement(4887..5630)
                     /locus_tag="VFQ61_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954946.1"
                     /translation="MRAKKKGEYRIRSFLNGQDHVTGRTFGDGSALMGRVYDKTLELK
                     QRHLPDSEKTLTEHAAWRAAGWDGKKPVTRVEFEMKRDALESFGIETADGLTDERLRE
                     LWSYLTGKWLRYSDGKAGRRERCQVDPRWHLIQSIPFATGVARPLERIHGRKRGATPE
                     QACGVVLSALSAVRALPKESTAKTVAHDVAMAFERMFEERFTPEQALLAVEGYRASQA
                     EIAEAMRNGQRPSACLPSKLREVLRKRSA"
     gene            complement(7201..7890)
                     /locus_tag="VFQ61_10610"
     CDS             complement(7201..7890)
                     /locus_tag="VFQ61_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954947.1"
                     /translation="MLQGKVQKPQQGRGEPRARAKLRAKRYATWKELQAATIEWCRLE
                     APRLNPRFNVADFERQARKWARFDDEPRRLWEALEAILERIEAFLVANPDSPSESIYA
                     GMLDRFMTSKIEGNDGKLTYSIRERLRHGRDRYRGTPRTSLRVKIVSLFSVGPNPIWP
                     DKKPRVRELAVLSLLAGCIPEGEKRRQEGAVGDADRPTIAEVLEAEQTNIRAVLKKLR
                     KEKKEEPAASG"
     gene            complement(8092..8904)
                     /locus_tag="VFQ61_10615"
     CDS             complement(8092..8904)
                     /locus_tag="VFQ61_10615"
                     /inference="COORDINATES: protein motif:HMM:NF039499.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HET9954948.1"
                     /translation="MSGDSHEDSLNLLKACSLNCKQLAVFVGQSVRPILDRELNALPY
                     RRICHALFLRVQAWLNSLSKLDEPGDFQAASAAARGIFETAIDMVLIQHQEGQSVIKL
                     MAWERSCKLKYAERHSAHSNELHVIEFVRDNKRAILSERESTWGKNKNGKPIAPERWT
                     GRNLEQDAKAADAYVPKASFVRFYSESYVPACWYVHGSGVVGILEVPAAFFPTLSALA
                     LHKVCVFGLVCTEYALRLFNVFDEIGQSRFARLQSEMGTLALAVSSRGLAKP"
BASE COUNT         1646 a         3061 c         2731 g         1683 t
ORIGIN      
        1 agcgcttcgc gcgcttctgg cgccagtgct cgatttggcg gcggacgggc tcgacgcgat
       61 cttcaggaag gcgggtcttc ttgcgtccca agggggctcc tgtcgtggcg ggcgtgaccc
      121 gcccatgcta cccgccgcga caccacaact catgcacgct caccggaagg acacaaacaa
      181 ctgggcgcct cgtgatcgcg gagggcgcgc cgctcacgtg ggtgcagcac gcgaacgacc
      241 tcgaagccgc cgagcggtgg ggagccacgt gccgcacggt cggcgccgac cacaacgaga
      301 ccatagtggc aatcctaaag tggcccaacg actgcaagga tctagcgaga cgcctcggag
      361 ggcgatggcc cattgtcgag cgctttgatg atccggatct gctgaagctc ggcgcgaaac
      421 tggccgacgc agacggtccc acagtagtcg ccgagctcgt cgactttgtg tccgagcgga
      481 tgaccggcgt cggcacggcc ttgaagacgg cggttgaggc gatcaaggcc ggtcggggca
      541 cgtcacgcat cactaagaac cggaggcatg ccgatcgcct cagtgcgctc gccagcgctc
      601 caacgccggc gagggcgctc gagtggctcg aaggtgtcct tgcgcaccgc gaagattggt
      661 ggctctaccg ccgtgaatgc gtttttcagc ttcgcgaagc actccgtcat tgcagcggcg
      721 ctactctagc cgagctgcct gacatggtcg cggcagcacg tactcgagcg cgtcaccgag
      781 gccgatacac ccaccgccga accatcggta cgccgctgct cgtgaagggc cttgagttcg
      841 accacgcagt gcttctctgg gagccgaacc atctctcggc ccaagggctc tacgtggcgc
      901 tgacacgcgc ctcgaagtct ctgactatcg tctcacgtac ccgcacgctc atcccggcgt
      961 gtgcgtgaaa gcgagccaac aaccgccttt caggacactc gctcgcgccg gcgacgcatg
     1021 tgcggtgcgc ggccgagtcg cttggcgccg gtttaagttc gagtaggcgt tggccgcgag
     1081 gtagccgacc tgcatgagca gccccttgcg ctcgccggga tcgaagtcga tgcgcgcgaa
     1141 gacccgctac ttgataggct gtcaatccgt ttcaccggtc gaagactgcg catcgagccc
     1201 gaacgtcacg acttcataca ttcgctcggc ccagtcgaaa atgatccctt cgaccgcggt
     1261 gcgagcggta gccacggtct cgggaaccac accccgttcc aaggcgccaa ccaactcgtc
     1321 gagcacaggc tctgcgtccg ccagcgccgg atcttcacat actattttcg taccattggc
     1381 gatgaatctg cctgacttga cggaatgaaa tccgcgaccg ggaaaccccc acgccacctc
     1441 caaaacacaa tatgccagtg caagggcaac ttcctgtggg tcgtgctctc gcaactgact
     1501 cagctttggt aaccggcggc ccagctctcg ctgtacgcct acaatctcct gccgaataga
     1561 atcacaaagt agcttcaccg ctccgtcgac cgcttgcagc ggcgccaacg cgttcggaaa
     1621 tgcgatcgac gccgcgtatc gaaatagagg gtcgcgtgtt gctacccgaa gttcggcgac
     1681 gcgcggctgg attttgaccc catgccgaac aacaaagctc tggtgaaagg ccgcagtcgg
     1741 gactctttga tcgtgatctt cgatggcaag gggccaaagc ggatcgctgc tccagtccga
     1801 ggtaacgcgg agagcgtcct cgatccaagc ccagagcggc ccgacaatag cacgctggca
     1861 gtgggcttcc cttgcgcccg tccaggaccg cgcaaggtgc tcacgccctt tcagcagcac
     1921 gttgagccga gaaggatccc agacgcaaaa cggcgcattg gcctttcggg acaaccatgc
     1981 atccctgagc tgcccgacga gcccgacagt gaccggaata ttcgtcagca agaggtagcc
     2041 gtcagcctcg gcgacacccg gcttatcgaa ttcccctttc ctttcgccag accgcaggta
     2101 gcacccgcct agccacgatc ggcgacgcga gacaccttcg atcggagatc ggaacttgta
     2161 ctggaataac caacgccccc ttactccgtc tacgagtccg tggtactcgg cgtcaacgcc
     2221 acgatctgct cctgcactcg cgaacgtctg cacaagatgg ccgaactcga aacgcagaag
     2281 cccatcgcag agcactttga agtctcgctc atgctggatg ctctcgacga tgcgccgcac
     2341 cacaggtatg ctccttccgc tgccaacagg gctccagggt cgccgaagca accgggctac
     2401 tgcaagcatg aaaacctcca tcaccgggac tgctctgcga tccggtgctt tcgctcttgt
     2461 gatgagttcc agagcaagcc aacgggtgcc cacaaccggc ggccggcgcg cccgttgcgg
     2521 gcgggacgcg cccgcgatcg atcccgcgag gcaacgggcg tgacggccgc cgatgcacga
     2581 gcccccaaga aaatgcggac ccggtggagt acaagaccga acagccgcac gccttgggaa
     2641 cctcgaaacg cacgtgttca tgcgcctgaa cccccgccgc aagcagagca tctcatgttg
     2701 cgcgtatgtc ccgcgacctc gccctttttg aaggatccgc ccggtaccag acgggtccag
     2761 ccaaacagaa atcgagcata atttccccca ctccgcatga atcacgcgag tcggcagatc
     2821 gcgcctccgg gttcgattcc cggcgcctcc accaaatccc gcctgttttt ggctagaagt
     2881 gccaccggca cggagccaaa agccgaaacc gacgccgaaa cccggcgtcg gttttttggc
     2941 tttgtgctga cgatcaggga cggcggcagc ggcgggaaca cctcgccgaa gccgtcgcgt
     3001 aggttctccg cctcgcggat gtagccctcg gtcgtcgaga acgtgcggtg ccccgcccgc
     3061 tgtttgactt tgaggggatc gtcaccgcgg acggcacacc aggtgattcc cgttgctcgg
     3121 aggtcgtgga acgtgatcgc cttgcgcgtc gggtcgccca tcacgaacag ctccgcccgc
     3181 ctcactcctg caagcgcgag gcagcggcgg agctggcgcg agagcttgcg atcggtagca
     3241 cggaccggcg cgacgactcc gcgtccgccg gtctcgtcgt gcatcgcacg cagcaatgga
     3301 atcagctctc gctcgatcgg gacgcgccgc gcatctcccg ttttcgtcgc cttccgggtg
     3361 ccggtgtctc ggttcacgct ggcgtggacg tggactaccg cgcgctccag atcgacgtcc
     3421 tcccaccgaa gagcgttcac ctcgccggct cgcgcgtaga ggtacgtcgt caccgcgaac
     3481 atgcggcgcc acgccagggg gacagcttcc gaagatacga gcgcggtgaa ctcgctaggc
     3541 cacagatacg acttcgcctt gtgcgcgccg atgtcaggtc cgggcacacc gtccgcgggg
     3601 ttgtcgtccc ttacacggag gtccacccgc tttgcgctgc atgcgtcgcg gaacatagcc
     3661 cgcgcgatgc tccagacatt cacagcggtt ttccacgcga acggccgcct tttgccgccc
     3721 gtctcgttgt agaaaccaac cctcgctttg ttgtcgagtg ccgacacgag acgccgcaca
     3781 tcatcgcgcg taacgtcgcg catgccgagc acgcctagta tcgggaacac atggcagcca
     3841 agcctcgcac cgtcgagctt tgcggatgaa aggccgcgtg cctcgcgcca cgaacaccaa
     3901 cgcgccgcgt actcggcgac ggtctcgcct gcgctcgcgt ctacggcaag gcgggcacgt
     3961 tcagccataa ccggagcgag cgtgagcgct tcttcgggcg acgtgccagg gtcgaagcga
     4021 atgacccgac ggcgcccatc ggcgcgcgtg acgcgcacgc cgcgcggccg tgtcttcgtc
     4081 tcggggtctt cccagacgat cgacccggtt gctggccttc caccgctgac ttttgctggt
     4141 atcttgtctg ccatcggagc tcaaccctcc ggtcatgccc ccggccgttg gtagcggcgc
     4201 gggggcttct tctttacttc gattcctgcc gatttgctgc tctgttattc gcctacttca
     4261 ccgtgaaatt gggctcgatg actttttggc gcggcgtgag tgcggcgccg accgtggcga
     4321 taccgcggcg cccaggacgt acctccgcga tctagtgcgg cgtcagcgcg gcgccagcga
     4381 gcaactggcc acaaactgcc tccgaactgg cagcgaacgt acgtccaagg gcaccttgtc
     4441 gatgtcaatg gacagctcgt gtgcgcgacg cctggcggcg actcggtgga cgctcggacc
     4501 gaggagcctc aaggcagcgt ctgtgttggc gtccgtacag cgacccaaca atccgcggac
     4561 tgcgaggcgg accttgcgca cgctgaggcg ggggcgattc aagagcacgt gaacgacggc
     4621 tgccgcaacc ggaagaagtc tttcgcgctc tgctttggac agcagcacgg ctgtctggga
     4681 atcggagatc tgaaacgaca ctttgcacca ccgacaatcg aggacgggac tccgtggaac
     4741 cccacccgga ggagcgatgt tcccgcctat gagggcaagt gagctgcggc acccgggaca
     4801 aactccattc gcaatgcatt cacaaagcgg agggcccaga gggacactac cggccaggtt
     4861 ccctctcgct tctctcggcc ggcccttcat gcactcctct ttcgaagcac ttcgcgcagt
     4921 tttgaaggaa ggcaggcgct cggacgctgg ccattgcgca tcgcttcggc aatttccgct
     4981 tgtgatgcgc ggtatccttc gacagcgagt aacgcttgct ctggcgtgaa gcgttcttcg
     5041 aacatccgct cgaacgccat cgccacgtcg tgagcgacgg tcttcgcggt gctctccttc
     5101 ggcaacgcgc gtacagcgga gagcgcgctc aagactaccc cgcatgcctg ctctggcgta
     5161 gcaccccgtt ttctcccatg gatgcgttcc agcggtcggg ccacgccggt tgcaaaggga
     5221 attgactgta tcaagtgcca ccgtggatcg acttggcacc gttcccttcg gccggccttg
     5281 ccatcgctgt atcgcagcca cttccccgtc agatacgacc acagttcgcg aagccgttcg
     5341 tcggtgagcc catcggctgt ttcgatgcca aaggactcga gcgcatcacg cttcatttca
     5401 aattccacgc gcgtcacggg ctttttgccg tcccaacctg cagccctcca ggcggcgtgc
     5461 tccgtcaacg tcttctcgct gtctggtaaa tgtctttgct tgagttcgag cgtcttgtcg
     5521 tagacgcggc ccatcagcgc cgagccgtca ccgaaggtac gccccgtcac gtggtcttgc
     5581 ccgttgagaa aggaacggat gcgatactcg cctttctttt tcgcccgcat gatccaacgg
     5641 gaggtctcgg tgttctcgaa gcctacgacg tcagcgcaca tgtcgaaccg ccggaggcgc
     5701 tcgcgaacgg gctcgtggga gccgccaccg ctaagcaccg acgcaagccg cggccccgtc
     5761 gtccgtagcg catcgacgat cgttcggccg atgcgcatgc actcggtgtg tcccttcgtc
     5821 gcgatggcca gagcttccca cttgatttcc acatcccagc cccgctcgtc gaggccgaca
     5881 aggagggtgc agtccccatt cctcagcagg aacgcgcccc cgcgtgcgcg gcgtgcgtcc
     5941 agcgcaaatg ggaacggccc aacgctgaat ggcacctgag ggactcgccc aggccccgcg
     6001 tgattcagcg cgacttctgc gtctcgaagc atttcgtcca tgcaggcgtc gaaatcagtc
     6061 ggagctgact cgcccgaggc cccgaagcgc accgcaccat tcgcccggtc gtaggctgcc
     6121 cggaaccacc cgagcgtctc ttggtccagg cccgcttggt atccgacggt gagcgcgtcg
     6181 actcggtgcc caaggacgca aactggacta gaatcggagc ctccgttggt gttctgcccc
     6241 ccctcccatg cgacggagcc ctggcctttc cttggcacca gtcttttcgg tgccttagct
     6301 ggcacggctg gtgtttgcga ccccgtgata ctcgacgggg tctcgaaggt ctttcgcccg
     6361 tccccgcggg gctctgcccc gcaccccgca ccagggggct tcgccccctc gccgctatcg
     6421 cggctcaccc ctgtcccctc gaaccgaacg ctgcctccct cgaccgttgc tgagtgcgct
     6481 cgtcgtccga cctggcctcg gtcggcaggc gccggctcga ggggacaccg cggccgtggt
     6541 gagcggccga ggccagggcg ggcgacaggg ctcaccgtgc gcctttcacg acgcgtaggc
     6601 cggcgcgagc gagcaacgcg tcggcctttg catcgagagt agtcggctca agcgatacgc
     6661 ccgatttcgg ccccgcgggc cgcacgcgcg ccgcatgcca ggcgccgcag gagcacaccc
     6721 actcgcggcc ctcgcgacgc gcaccaacga tgcacccgga gcgacagagc tctgcaaaac
     6781 ggcgacgcga acaacgcggc ggcagatcgc gagacgagta cgtctcacgt tgccccgcag
     6841 tcaaaatgcg cgctgcgatt cgatcggcga gcgaatcaat caatgcgtcg atggtgtcgc
     6901 gcagggaaga tgtgcccgtt gcgctggcgg agcacgtggt catcgcgtcc gctcgcggag
     6961 gggcggcgtc gctgtttcgg tgcgtagctc acgaatcgca cgctcgatgc gagccgcggt
     7021 cgttgagtgc tggggacggc tgtcgaggta ggcgcggacc gtgcgcggat cgcagaaagc
     7081 gcgggtagct acctctcgga gttcgtgagg cgtcaggttt gcgtgagaca cgctgcaaga
     7141 ctgcggcggt cctgaggcac gagtacccgt gccccgagca ccaccttcgg ttacgggggt
     7201 tcagccgctc gcggccggct cctctttctt ctccttccgc aactttttca gcaccgctcg
     7261 aatattcgtc tgttcggctt ccagcacctc ggcaatcgtt ggccgatcgg cgtctcccac
     7321 tgctccctcc tggcgacgtt tctcgccttc ggggatgcat cccgccagta gcgacaagac
     7381 agcgagctcg cgaacacgcg gtttcttgtc gggccatatc gggttgggcc cgacggaaaa
     7441 gagcgacacg atcttcacgc gcaagctcgt gcggggcgta ccgcgatagc gatctctgcc
     7501 atgcctcagc cgctcgcgaa tcgaataggt gagcttgccg tcgtttccct caatcttgga
     7561 tgtcatgaat ctatcgagca tccccgcgta gatgctttcg gagggggaat ccggattagc
     7621 gacgagaaac gcttcgatgc gttcaaggat cgcctccagc gcttcccaaa gccggcgcgg
     7681 ctcgtcatcg aagcgggccc acttccgcgc ctgtcgctcg aaatccgcga cattgaacct
     7741 cgggtttagt cgtggcgcct ctagccgaca ccattcgatt gtggcggcct ggagttcctt
     7801 ccaggtcgcg tatcgcttag ctcggagctt tgcccgcgca cgaggctcgc cgcgaccttg
     7861 ctgcggcttc tgcacctttc cttgtagcat tgatgaaagt ccgatcatgc actgctggtg
     7921 agacagccgc tcgggccgtg caccgcgtct gccagcagcg tcgaggctga cacgacatcg
     7981 ccgcgttcgt caacgcccgg acagcgtcac cgcgcgtgac cccgcccacg agaggtggcg
     8041 tgcaatgagc agaataagca aaccccgctc ccttggatcc atgccgatgc gtcacggctt
     8101 cgcaaggccg cgactcgaca ccgcaagtgc tagcgtgccc atttcgcttt gcagccgagc
     8161 aaagcgtgac tggccgattt catcgaacac gttgaagagc ctgagcgcgt actcggtgca
     8221 cacaagtcca aaaacacaga ccttgtgtag cgccagcgcg ctcagcgtag gaaagaacgc
     8281 cgcgggaacc tccaatatcc cgacaacccc actgccgtgc acgtaccagc aggctggcac
     8341 gtagctctcg ctgtagaacc tcacgaagct cgccttcggt acatacgcgt cggctgcctt
     8401 cgcgtcctgc tcaaggttgc gcccggtcca ccgctccgga gctatgggct ttccgttctt
     8461 gttcttaccc cacgtcgatt cgcgctctga caggatcgcg cgcttgttgt ctcgcacgaa
     8521 ttcgatcacg tgcaattcat tgctgtgggc cgagtgacgc tccgcgtact tgagcttgca
     8581 ggagcgttcc caagccatga gcttgatgac cgactgccct tcttggtgct gaatgagcac
     8641 catgtctatt gcagtttcaa agatcccacg cgcggctgcg ctcgccgcct ggaaatcgcc
     8701 aggctcatca agcttgctga gtgagttgag ccatgcttgg actcgaagga atagcgcatg
     8761 gcagatccta cgatacggga gagcattcaa ctcccggtcg agaatgggcc gcacgctctg
     8821 acccacgaaa acagccagct gcttgcagtt gagcgagcag gccttgagca agttgagtga
     8881 gtcctcatgt gaatcgccgc tcattgcgca attgtacctg aagcggcgca gctcgctccg
     8941 ccgcaacgat gacacgaccc aatctggcgc ctcccaggtt acatcgtgtg cttccggcaa
     9001 gcgtgcatga gccgttgccg cgcgtcgggt agcgtgagcg ggtccacccg ccacgacagg
     9061 agcccccagt gggacgcaag aagactcgcc tgctcgaccc ccgcgtcgag cctctccgca
     9121 a
//
LOCUS       DASTKU010000295         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1842401, whole genome shotgun sequence.
ACCESSION   DASTKU010000295 DASTKU010000000
VERSION     DASTKU010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842401"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..1202)
                     /locus_tag="VFQ61_10620"
     CDS             complement(285..1202)
                     /locus_tag="VFQ61_10620"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9954949.1"
                     /translation="MRIFITGATGFIGSHVAFELLNAGHELVAAARNPDKLPELSRHP
                     RVHRVTARLTERAPMRDALQGCEACVHIALGWGDTPVDMLMQDTLPSVALLEDCAAAG
                     VQRFVYTSSTAALGPFFERMNEAHPTRPNDYYGATKAATESFVLAVGAKTGMRCNVIR
                     PGYTFGNPVVAGAPTQPDPRFRDIARAARENREIRVTRGDGTQFVWAGDLARLYRAVL
                     ESEHQREVYYGLGQSFVSWEQVAARARELSGSSSPIVLEEPREPVPPGRDARPNLFEL
                     SKIAEHFGLSFDAQPRIDEHLAYFLAEPS"
     gene            complement(1299..3380)
                     /locus_tag="VFQ61_10625"
     CDS             complement(1299..3380)
                     /locus_tag="VFQ61_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9954950.1"
                     /translation="MSDRQQPITSLHEHASSGEHIGTPDSNLERTPHEWAAREALLRQ
                     LMQERARLSRELGERVKELSLLHQASRLLRRAGPVDLTLLQDVCLMLPAAWQYPEVCE
                     ARIRYGDLDARTAGFRGSDWRQEARFRTADGRVGVIEVVYLEERPTAGEGPFLLEERR
                     LIQSLADMLAVHADQRCVEAELRHSQQKFATVFLAAPVALCITRQADGQMLDLNYEFE
                     RLFGYSRAEAIGHTTIELGMWPNPDDRKAYVYEIPSGVLHVAEMRLRHRDGRIIPLRN
                     SLHRLDLNGETCVLSAFIDMSAQLERQALEQQLQHAQKMEAVGRLAAGVAHDFNNLLA
                     VITMCSDVLREDLPANSGAQTELLEIGEAAERAEHLTRQLLAFSRQQVLEPRLLSANE
                     LVSKLQNMLQRVLGADVELRTKLDPACGMIKADAGQLEQVIINLALNGRDAMPEGGQL
                     QIATACCDAPPEAGQSSGPWLVLSVRDTGTGMDAETRARIFEPFFTTKPPGKGTGLGL
                     STAYGIVRQSGGVLDVTTELGRGSTFRIFLPCADGSEELPVTPTLQRRTSGGNEVVLV
                     VEDQPAIRHVIHKILARLGYQVLEAASPHLALEVSRRHSGPIHALLTDLVLPGGSGRK
                     LGAELLLQRPRLRVLYMSGFTDDAAVSGGALEPGSAFIQKPFSAAMLAARLREVLDRA
                     PRSSTVDHRAG"
BASE COUNT          659 a         1163 c         1175 g          686 t
ORIGIN      
        1 cgccacgata ttgcgccaaa ggtaagacga gctcgggttg ccatgcagaa acagcaacac
       61 tggccctttg ccttggtcga cataggccat ctggtggtca cggacggtca cgaaatgctt
      121 cgaactcgag gcgctcatgg ggaatctcct gagcccgagg tccaaatgct gggctcaccc
      181 ggttggagac gggggctcgc gaaaacgtga ccagagcacg gccttttttt ccaaatctct
      241 ggcaggcccc ttggctcgcg tcccatctcg agccgagccg gggctcagct cggttcagcc
      301 aagaagtagg ccaaatgctc gtcgattctc ggctgggcgt cgaagcttag gccaaaatgc
      361 tcggcaatct tgctgagctc gaacaagttc gggcgggcat ctctgcccgg cggcacgggc
      421 tcccggggct cttccagcac gatggggctc gacgagccgg aaagctcgcg agcgcgcgcc
      481 gccacttgct cccagctgac gaaggactgc cccaagccgt agtagacctc gcgttgatgc
      541 tccgactcga gcacggctcg atacagccga gccaaatctc cggcccagac gaactgcgtg
      601 ccgtctccgc gcgtcacgcg aatttcgcgg ttctctcgag ccgctcgggc aatgtcgcgg
      661 aagcgggggt ccggctgcgt gggcgcgccc gcgactacgg ggttgccgaa ggtataaccg
      721 gggcgaatca cgttgcagcg catgcctgtt ttcgctccca ccgcgagtac gaatgactca
      781 gtcgcggcct tggttgcacc gtagtaatcg ttgggacggg tcgggtgcgc ctcgttcatg
      841 cgctcgaaga acggacccaa cgccgccgtc gacgaagtgt agacgaaccg ctgcaccccg
      901 gccgcggcgc agtcctcgag caatgcaacg ctggggagcg tgtcctgcat caacatgtcg
      961 acgggcgtat cgccccaacc cagcgcgatg tggacgcacg cctcgcaacc ttgtagcgca
     1021 tcacgcattg gggcgcgttc cgtgagtcga gccgtcaccc ggtgaacgcg cggatgccgt
     1081 gagagctccg gcaacttgtc tggattacga gcggcagcca cgagctcgtg gccggcgttc
     1141 aacaactcga aagcgacatg agaaccaatg aagcccgtag ctccggtgat gaagatgcgc
     1201 acgcgatcgc tccgcctggc tgaggtggtg tgcaggccgg cccagcatac tcgcaaaatc
     1261 acccccgggt agttcgccgc atctcggccc ggccgtgatc agcccgcgcg gtgatctaca
     1321 gtggacgagc gaggcgcgcg gtccaaaacc tcgcgtaggc gcgccgccag catcgccgcg
     1381 gaaaacggct tctggatgaa cgccgagcca ggttcgagcg cgcccccgct caccgccgca
     1441 tcgtcggtaa agcccgacat gtagagcacg cgcagccgtg ggcgttgaag caagagctcg
     1501 gcccctagtt tgcgaccgct gccaccgggc agcaccagat ccgtgagcag cgcatgtatc
     1561 gggcccgagt gccgacggct cacctccaac gcgagatgtg ggctggcggc ttccaagacc
     1621 tggtatccaa ggcgcgccaa tatcttgtgg atgacgtgcc gaatcgcggg ctggtcttcg
     1681 accaccaaca ccacttcgtt tccgccggac gtgcggcgct gcagtgttgg ggtcactggt
     1741 aactcttccg agccgtctgc gcagggcaag aatattcgaa aggtgctgcc tcgcccgagc
     1801 tccgtggtca catccagcac gccgccgctt tggcgaacga tgccgtaggc ggtggacagg
     1861 ccaagtcccg tgcctttacc tggaggtttg gtggtgaaga agggttcgaa gatccgggcg
     1921 cgggtctcgg cgtccatccc ggttccggtg tcacgcaccg atagaacgag ccacggtccc
     1981 gagctttgtc cggcttctgg aggtgcgtca cagcaggccg tcgcgatctg cagctggccc
     2041 ccttcgggca tggcgtcgcg cccgttgagc gcgaggttga tgatcacttg ctcgagctgc
     2101 ccggcatcgg ccttgatcat tccgcacgcc gggtcgagtt tcgtgcggag ctcgacatcg
     2161 gccccgagca cgcgttgcaa catgttctgc agtttggaaa cgagctcgtt tgccgagagt
     2221 agcctcggct cgagcacttg ctgacgactg aaggcgagga gctgacgagt taggtgttcg
     2281 gctcgttccg ccgcttctcc aatctccagc agctccgttt gtgctcccga gttggccggt
     2341 aagtcttcgc gcagcacatc gctgcacatc gtgattacgg ccagcaggtt gttgaaatcg
     2401 tgcgctacgc cggcggctaa tcgacccacg gcctccatct tttgcgcatg ctggagctgt
     2461 tgctcgagcg cctgccgctc gagttgggcg ctcatgtcga tgaaggccga gagcacgcag
     2521 gtttcgccgt ttaaatctag tcggtgcaac gagttgcgga gcggaatgat gcgcccgtcg
     2581 cggtggcgga gacgcatctc cgcaacatgc aagactccac tcgggatctc gtacacgtaa
     2641 gccttgcggt cgtcgggatt gggccacatc ccgagctcga tcgtcgtgtg gccgatcgct
     2701 tctgcacgcg agtagccaaa cagcctctcg aattcgtagt tcaaatcgag catctggcca
     2761 tctgcttgac gagtgatgca gagcgccacc ggggctgcca aaaatacggt cgcgaacttc
     2821 tgctggctgt gccgcagctc cgcctcgacg caccgctggt cggcgtgcac ggccagcatg
     2881 tccgccagcg attggatcaa ccgccgttct tcgaggagaa acggcccttc ccctgcggtt
     2941 gggcgttcct ccagatagac cacctcgatc acacccacgc gcccgtccgc ggttcggaag
     3001 cgcgcttctt gtctccaatc actgccgcgg aatccggccg tgcgcgcgtc gagatcgccg
     3061 taacggatgc gcgcctcgca gacttccgga tactgccaag ccgcgggcag catcagacat
     3121 acgtcttgga gcagcgtcaa atccacgggg ccggcgcgtc gaaggaggcg tgaagcttgg
     3181 tgcaaaaggc tcagctcctt cacgcgctcg ccgagctcac ggctcaagcg tgcgcgttcc
     3241 tgcatcagct ggcgaagtag ggcttcacgc gcggcccatt cgtgaggcgt gcgttctaaa
     3301 ttggagtcag gggtgccgat gtgttcacca ctactcgcgt gttcgtgcag cgaagtgatg
     3361 ggctgctgtc gatcgctcac gagagcctcc gtgcggatgc tacgtcggtc tcggtcggag
     3421 aagaagaatt tctcctcgcc gcgtcccgag gcacagtggc agcgctgtcg gaaaccgtcg
     3481 cggagcttca cggcgcaccc aagttggtgg gctgggcgag tccacgagcg gagagggcgc
     3541 ggatccgtgg tcggcgcatc atgaacgacg atgtaggtcc gagttgtcgt ggtgcatcac
     3601 ccgctgggta gagtcgtgag cggcaaatcg actgcagctc caggccgcgt actcatcggc
     3661 gcgccgcgtg atgctaccct cga
//
LOCUS       DASTKU010000296         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_504563, whole genome shotgun sequence.
ACCESSION   DASTKU010000296 DASTKU010000000
VERSION     DASTKU010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504563"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFQ61_10630"
     CDS             complement(<1..500)
                     /locus_tag="VFQ61_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9954951.1"
                     /translation="MSTFVLIHGGGDVGWSWHLVEAELRARGHEVVAPDLPGDDDSLE
                     LEDYADAVLAAVGDRRDLVVVGHSFGGFTAPLVAARRPVVALVFVAGMVPSPGEAPNA
                     WWERTGYSKAVQEQAALDGGVTGNEDPYVCFYHDVPRELAKQALSKERAHPSAASGAS
                     PWPLEAL"
     gene            850..1764
                     /locus_tag="VFQ61_10635"
     CDS             850..1764
                     /locus_tag="VFQ61_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9954952.1"
                     /translation="MSELVKAVAEARRNFMDLVAEVRPELHRYCARMTGSVFDGEDIV
                     QDTLAKAYYALAELESVPPLRPWLFRIAHNTAIDFLRRYDQRFLDPLAEVAESSLAAE
                     PELDGTRVEAALQRFTALPPLQRSALALKEVLGYSLEETAVVMGTSVPAVKAALVRAR
                     ANLARANPPSNEPVELAELGRLRRYADLFNARDWEGLSQLFGEETQLDVVSREQRRGR
                     AATKYFTYYAKLAPVEELRAQVGQVDGEPALAIFRAGADRQPAYFIRLEWREGALVRV
                     SDWRYVPYIADSAVFTPSDAMENFRSDS"
     gene            1819..2577
                     /locus_tag="VFQ61_10640"
     CDS             1819..2577
                     /locus_tag="VFQ61_10640"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9954953.1"
                     /translation="MMTTLQDQKVIVTGGSRGLGLGIVEALVEQKARVTVVARNAAPL
                     EQVSRRLGVHAIQGDITDRELAAGLLREIRPSVLVLNAGATPVMGSLLDLTWAEFSGV
                     WDVDVKAGLQWVQEALRLPLPRGSQVILSSSGAALAGSPLSGGYAGAKRMLWMLASYA
                     NTVSQRLNLDLRFKALIPQQMIGETELGHSAASAYAAQKGISVETFLDGFGARMPPRK
                     VGEHVVSILTDPRHRTGVAFGIKGDHGIVSLDGN"
     gene            complement(2547..>2999)
                     /locus_tag="VFQ61_10645"
     CDS             complement(2547..>2999)
                     /locus_tag="VFQ61_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954954.1"
                     /translation="ATRNVESPRDVQSFWNFVEQSSTRGFEFMLEDPVAANLMQCLQR
                     EQSLLAELRSSDLLESIDEFYAELTRRGQELGAVRVDLPAELLVALVRDLAMTFDRWF
                     ITARAGAANGKPGPSPAAAARAFTDTAQRLCRPTRAAHAQLPSSDTIP"
BASE COUNT          525 a          891 c         1027 g          556 t
ORIGIN      
        1 agcgcttcga gcggccacgg agaagcgccg ctcgcggccg aggggtgggc ccgctctttg
       61 ctcagcgctt gctttgccag ctcccgtggc acgtcgtgat agaagcagac ataagggtct
      121 tcattgcccg ttacgcctcc gtcgagcgcc gcttgctcct gcacggcttt cgaatagccc
      181 gtgcgctccc accaggcgtt gggcgcctca cccggagaag ggaccatgcc ggctacgaac
      241 acgagggcga ccacgggtcg ccgcgcggcc acgagcggag ccgtaaaacc tccgaaagag
      301 tggcccacca cgaccaagtc tcggcgatcg cctacggccg cgagcaccgc atctgcgtag
      361 tcctccagct cgagggaatc atcgtcccca ggcaaatcgg gtgcgacgac ttcgtgcccg
      421 cgtgcgcgca gttcggcctc caccaggtgc caagaccaac caacgtcccc cccgccgtgg
      481 atcaaaacga atgtgctcat gtgcgtagct ccgctctccg tgcatgacac agaaggctac
      541 gctttaaaac gcgccaaatt ccgcatgtcg cggcgggcgc aggcgcggtg cggctggatg
      601 catgcgaggg caggggctgc gctggcggtt ggatgggtgg gcagtcgcgc tcgttcactc
      661 tttggcgtcg aagagcagag tcgttccccg gtcgaggcag ccggtttggg ggtagccgtg
      721 ctcacgggtg ctccggcaca gtcgtaccct gggccggact ctgcgttgcg agattcgtac
      781 gcgtgagcgc atccttttga gtcgctgaat cgtcatggct cgaggcgcat gcaaaaggat
      841 actgaacgga tgagtgaact cgtgaaggcc gtggctgagg cgcgccgcaa cttcatggac
      901 ctggtggcgg aggtacgccc cgagctacac cgctactgcg cgcgcatgac cggatcggtg
      961 ttcgacggcg aagacatcgt acaggacacc ttggcgaagg cttattacgc gttggcagag
     1021 ctggagtccg tcccgcccct gaggccgtgg ttgtttcgta tcgctcataa cactgcgatc
     1081 gacttcctga ggcgctacga ccagcgcttc ctcgaccccc tggcggaggt ggcagagtct
     1141 tccttggcgg cggaaccgga gctggacgga acccgcgtag aggccgctct ccagcggttt
     1201 acggctctgc cgcctctgca gcgcagcgcg ctcgccttga aggaggtgct gggctactcg
     1261 ctcgaagaga ccgcggtggt gatgggaacg agtgtcccgg cggtcaaggc cgcgttggta
     1321 cgtgcgcgcg ccaatctcgc ccgcgcgaac ccgccgagca acgagccggt ggaactcgcg
     1381 gagctcgggc gcttgcgccg ctacgccgat ttgttcaatg cgcgggattg ggaggggcta
     1441 agtcaactgt tcggggagga aacccagctc gacgtggtct cacgcgagca gcgacgtggg
     1501 cgggccgcga ccaagtactt cacttattac gcgaagctcg ccccggtcga ggagctgcgc
     1561 gcccaggttg gacaagtcga cggtgagccg gccctcgcga tcttcagagc gggagccgac
     1621 cgtcaaccgg catacttcat tcgactggag tggagggagg gtgccctggt ccgagtttcg
     1681 gattggagat acgtgcccta cattgcggac tcggctgtct tcacgcccag cgatgcaatg
     1741 gagaactttc gctccgattc gtgactggca gccgcatcct tccggcgccc tggatcgtgt
     1801 tctaaccgaa ggagcacgat gatgacgacg ttgcaggacc agaaggtgat cgttacgggt
     1861 gggagccgcg ggttgggctt ggggattgtc gaagcgttgg tagagcaaaa agcgcgtgta
     1921 accgtggtgg cgcgcaacgc cgcccctttg gagcaagtct cgcgaagact cggggtgcac
     1981 gcgattcagg gggacattac ggatcgagaa ctagcggccg ggttgttgcg ggagattcgc
     2041 ccttccgtgc tggtgttgaa tgcgggcgcg accccggtga tgggctcgct tctggatttg
     2101 acttgggccg agttcagcgg cgtgtgggac gtcgacgtca aagccggtct tcagtgggtc
     2161 caagaagcat tgcgcttgcc gctgccacgc ggaagccaag taatcctgag ttcgagcggg
     2221 gccgcgctgg ccggctcgcc tctttcgggt ggttacgccg gtgccaagcg aatgctttgg
     2281 atgttggcga gttacgcaaa tacggtgtcg caaaggctga atctcgatct tcgtttcaag
     2341 gccctcattc cgcagcaaat gatcggagag accgagttgg gtcacagcgc ggcttcggcc
     2401 tatgcggcgc agaagggtat tagtgtggag acgttcctcg acggcttcgg ggcccgcatg
     2461 ccgccgcgaa aggtcggcga acacgtggtc tcgattctca ccgatccgcg ccatcgaacg
     2521 ggtgttgcat tcgggatcaa aggtgatcac gggatcgtgt cgctcgacgg caactgagcg
     2581 tgcgcggctc gagtgggacg gcatagccgt tgggccgtgt cggtgaaggc gcgcgcggcg
     2641 gcagcggggc tcgggcccgg tttgccgttc gcggcacctg cgcgtgcagt gatgaaccag
     2701 cgatcgaagg tcatcgcgag gtcccggacg agcgcgacga gaagctctgc gggtagatcg
     2761 acccgcaccg cgccgagctc ctgcccgcga cgggtgagct cggcgtagaa ctcatcgatc
     2821 gactcgagca gatcgctcga gcggagctcc gcgagcagcg attgctcgcg ttgcaggcac
     2881 tgcatcaagt ttgcggcaac cggatcttcg agcatgaact cgaacccgcg ggtgctgctc
     2941 tgttcgacga agttccagaa actttggaca tcgcgcgggc tctcgacgtt gcgagtggc
//
LOCUS       DASTKU010000297        12115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1842871, whole genome shotgun sequence.
ACCESSION   DASTKU010000297 DASTKU010000000
VERSION     DASTKU010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12115
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1842871"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VFQ61_10650"
     CDS             complement(<1..857)
                     /locus_tag="VFQ61_10650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9954955.1"
                     /translation="MSTTQASRQEIDDVSDTARWVAAYRAWETKRRDALFHDPYAARL
                     AGEKGQRIARGMRGFNGSHNGWPIVTRTKILDDLILSAVQDGCTCVLNLAAGFDTRPY
                     RLPLPADVRWFEADLPTILEEKEVELANEQPRCALHRIAVDLTDPIQRAALFDRVDQA
                     GRSTLVITEGLVVYLEPSSVHELAVALFARANFTHWALDFSSPAILRDLQRSMGQALT
                     RAPMKFAPADGLQFFETIGWRAQRVHSLFHEGARLRRVPWPLRLLSAFPPPPAHALGK
                     NRWSGVVCLG"
     gene            complement(1034..1804)
                     /locus_tag="VFQ61_10655"
     CDS             complement(1034..1804)
                     /locus_tag="VFQ61_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9954956.1"
                     /translation="MTNSSRPRIALITGGSRGLGRSMALHLADHGVDSIVTYRSARTE
                     ADQVVAALRAKGRLAAALPLDVSDSATFPSFVTGVREALRQTWQRERFDFLVNNAGTG
                     LHAKFTETTEQQFEELVKVHLKGPFFLSQQLVPWIEDGGRILNVSSGLARFTFPGYSA
                     YAAMKGAIEVLTRYMAKELGARRITVNTLAPGAIETDFGGGAVRDNAELNRIIASQTA
                     LGRAGLPDDIGGAVAALLSPGCHWLNGQRIEFSGGMML"
     gene            1918..2817
                     /locus_tag="VFQ61_10660"
     CDS             1918..2817
                     /locus_tag="VFQ61_10660"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9954957.1"
                     /translation="MSLDLETLRVFVKVAELGSFTRAGQHLRLSKSRVSLRVQELETR
                     VGSRLLQRTTRAVQLTPDGEQFCERARRLLNDAEELAAFLQAPSALRGRVRLDLPIAL
                     ARNLVIPRLPEFLTAHPQLELELSTTDRRVDLVQEGFDCVVRIGNLADSGLVARRLGE
                     LDLVNCASPSYLLKRGIPRSLADLAKHQVVHYSLRFGADAPSFEYHDGERYRELPMAS
                     VITVNNGDAYRDACLAGLGITQIPRIGARSSLASGALVEILPDFAAAPMPIWLVQGHT
                     RNVPKRVRVVMNFLAQLIERDYG"
     gene            3028..4329
                     /locus_tag="VFQ61_10665"
     CDS             3028..4329
                     /locus_tag="VFQ61_10665"
                     /inference="COORDINATES: protein motif:HMM:NF025268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9954958.1"
                     /translation="MSTSSSALIAPASGESQPAARWFEDRAQAAFELAEQRGQLVFAD
                     LWAPWCHTCLSMREHVLDVRRVPSLGRVVLLAIDTERAENASFLQQYPVGVWPTFYLI
                     DPRTRSVRGRWLGAASPEQLDRFIGDGAQPGSQAAILLSEADAAAAKRAWLQAAKFYR
                     GALESAPAGWPRRGDVLVSLASSLYKQESFEECLQLALSAVQWVPPSISAADFAATTL
                     GCAERAGEGSKDSARRVRQALAGLLQGLCASAAPGASPDDQADACGSLRRAREALGDR
                     EGARQAAEQGLAVIERATLGAKPDSQLIYDWERTSNLVYLGRKREALELLMQRERDCP
                     QSYNPAHYLARLYRDAGEWEAGLAAIERALAKAYGPRRAGLVGVKVDLLRGAGRTSEA
                     RATLEQQLSEYRALPEGQKQPEAEATVQKRLSDWPLPQLEH"
     gene            complement(4342..4755)
                     /locus_tag="VFQ61_10670"
     CDS             complement(4342..4755)
                     /locus_tag="VFQ61_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954959.1"
                     /translation="MAELTTRTARVWLGEDDGLVHLQPLARREQTLDDAVENVQAVKS
                     VCAGVVRPLLIHFEAATAQTPECRAHYLSDEATSAVSAVAIVTNSLLGRIVGNLMIGM
                     HTRGAPVRLFDCTEKASAWLASYLPTVARVAGARR"
     gene            complement(4968..5921)
                     /locus_tag="VFQ61_10675"
     CDS             complement(4968..5921)
                     /locus_tag="VFQ61_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET9954960.1"
                     /translation="MTRPNSSVTQVSAPALGVPSPAGARPTIKTRGLSGIYSAPPLHW
                     VGDGFRVAGYLSQIPDAVRQLSPFLLLDYHPPYEYPPTRRARGVGVHPHRGFETVTLA
                     WQGSVAHHDSAGGGGVIGPGDAQWMTAASGVLHKEYHEQNFAERGGVFHMAQLWVNLP
                     RAHRMAPPRYQGILASDMGRVDLPHAAGSVRILAGEYGNARGPAKTFTPISIFDLTLT
                     ARGSADFTIPSKHNLAALIMQGNVRLNDAPAREMDFAVFANDGDRLHIEADSDAQVLL
                     LGGEPIDEPVVQYGPFVMNTEPEILEAFRDFSSGRFGHLAD"
     gene            complement(6031..6507)
                     /locus_tag="VFQ61_10680"
     CDS             complement(6031..6507)
                     /locus_tag="VFQ61_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9954961.1"
                     /translation="MTRTTLRTVDRFAHSHAQYAPTLLRLGLGAVFLAHAYAKLTIFT
                     LPGTRAFFEAHGFPGWSAYPVFALELIGGVSLLLGLYSRVAALALIPVMLGALVPHLA
                     NGWMFTNSGGGWEYVAFLLIALFTQLLLGDGAFALSHGRAAEKGTNWESPSVRAAS"
     gene            complement(6622..7542)
                     /locus_tag="VFQ61_10685"
     CDS             complement(6622..7542)
                     /locus_tag="VFQ61_10685"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET9954962.1"
                     /translation="MPDPLLDDLLALLYLVRVVECGSFTKAAAKLGVSKSVVSEKVSA
                     LETRLGEQLLLRTTRNVTPTHAGTRVYSHARQMVEAGRQATRDSSAASGGVVRLSAPV
                     SLGQLFLARPIAEFLSAHPRSRIELLLNDRLVDLIEERIDLALRITKLKDSSLLARRL
                     GWTTIHVCGSPSYFAEHGRPKRPEDLVRHNCMRYAYLRPDHEWRLYSEGGRVPLELTG
                     SFETTNGTMLREAAIAGAGLAILPRFMIADALRAGTLETVLDSFGPRPMGIYAVRSGR
                     PTPPKLVAELIAAFEAAFQGPDWQAPVPRA"
     gene            7690..8103
                     /locus_tag="VFQ61_10690"
     CDS             7690..8103
                     /locus_tag="VFQ61_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9954963.1"
                     /translation="MELTHIRLLVYDFEQTFKFYRDLIGLKPQADTPQGPYAKFSFAE
                     GAAAISLQQRSHFIENSVPLRERSSGLAAGTDNALIAIRVASVAEEVERLRQSGAEVT
                     GPRDLFGRMTCAWLRDPEGNLIELQEWVNAGARKS"
     gene            complement(8078..9211)
                     /locus_tag="VFQ61_10695"
     CDS             complement(8078..9211)
                     /locus_tag="VFQ61_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET9954964.1"
                     /translation="MAKSQPTPAAKLKNILDGRFVEQLASELQAAHPQFDAKRFTRAS
                     RQGLEELELTPRAWHVAEALHQYLPQPFPRAAEVLIRSLRPELADTESLGLSPLQYLP
                     HVFYVQKYGLDHFEPAMALQRELTKRFSAESSIRAFLVRHPEATYARLQQWALDPNVH
                     VRRLVSEGTRPRLPWAPRLRAFQQDPRPVLELLELLKDDSERYVQRSVANNLNDIAKD
                     HPDLVLDTCERWNAGASTSRAWIIRHGLRSLIKQAAPRALALVGAGKRARVEIEAVRL
                     TPPRPKLGETVRFEVALRSISTREQDLLVDYVVHFVKFQGHTRPKVFKLKRLLLEPKS
                     RTELRGKISFAPMTTRQHYPGTHRLELLVNGAPYPLTTFELRR"
     gene            9275..10039
                     /locus_tag="VFQ61_10700"
     CDS             9275..10039
                     /locus_tag="VFQ61_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET9954965.1"
                     /translation="MVTESVTIVSRAERLLQLSDLLRGTEGSTVAQLANQLGVSRRTL
                     LRDLASLRDRGMPISSDVGRGGGVRLEGDRGLTALHLSVSEVVAIWLAARLSREASEL
                     PWAEAANSGMAKLLASLPKPKSRALRALCRRVIVGRPASANVRAGIGAPPPELLRLFE
                     EAFSAGLGLAFHYTDREQKVSIRRVEPHGLLVETPAWYVLARDIDKAEPRMFRMDRIA
                     RARILPDVEFKPDLRVIQAQFADLSGWHPLAGHWLT"
     gene            complement(10065..10700)
                     /locus_tag="VFQ61_10705"
     CDS             complement(10065..10700)
                     /locus_tag="VFQ61_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9954966.1"
                     /translation="MTSLRSYEDPCGIARALDVVGERWGLLIVRELVFGPKRFTDLRT
                     GLRGASPNVLSQRLRELEAAGVVQKRADTAGTYELSDWGRELHPILVALGRWGAQSPV
                     RPAGELSVDALIIALETTFEPNESWTDRYVFELRLSGDSFILEVEGPNLTITRGTPGH
                     PNAQILSDPATLRAVVFGGRSTALGGIELRGDRRLAKRLLRQFPRPNMIQR"
     gene            10814..11380
                     /locus_tag="VFQ61_10710"
     CDS             10814..11380
                     /locus_tag="VFQ61_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9954967.1"
                     /translation="MRRVRVFENVSLDGYFCDERGDMSWAHRSDPEWNTFVAENASGE
                     SQLVFGRVTYQMMAGYWPTPMAFQQNPRVAERMSATKKYVCSRSLSAADLGWQNSELV
                     SDLVSGVQALKREAGPDLVILGSGSLVAQLSEAGLVDEYQVVLQPIVLGKGKSLFSGV
                     THRIPLTLKRSRCFGNGNAVLWYEPSVG"
     gene            11502..11879
                     /locus_tag="VFQ61_10715"
     CDS             11502..11879
                     /locus_tag="VFQ61_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954968.1"
                     /translation="MRQVDPLLRWCENPFFLLGLMPTASERDIEREGRKLLGQLELGL
                     RSARWARTPLGEVERTPDSIRSAIAELRDPQKRRVHAIWAALPIHSPGVWGPTDSEGH
                     APPTGVTEEPFLEAMSAAGWPGL"
     gene            11936..>12115
                     /locus_tag="VFQ61_10720"
     CDS             11936..>12115
                     /locus_tag="VFQ61_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954969.1"
                     /translation="MFPVGAIVAALFAARRSSPAMKPSRALVLGSKYVVQWQGEEKHM
                     TWSLALVLIMPLALVA"
BASE COUNT         2237 a         3657 c         3955 g         2266 t
ORIGIN      
        1 cccaagcaga ctacgcccga ccagcggttt ttgcccaagg cgtgcgcggg gggcggcgga
       61 aaggcggaga gcagccgcag aggccaaggc acgcgacgca gccgcgctcc ttcgtgaaaa
      121 agggaatgca cccgctgcgc acgccagcca atcgtctcga agaactgcag gccgtctgcc
      181 ggagcaaact tcatcggcgc ccgcgtcaat gcctggccca tgctgcgctg taggtctcgc
      241 aaaatcgcgg gtgaggaaaa atcgagcgcc cagtgcgtga agttcgcgcg agcgaacaac
      301 gctacagcga gctcgtgcac ggagctcggc tccagataca ccacgagccc ctccgtgatg
      361 acgagtgtac tgcgaccggc ctggtccact cggtcgaaca aggctgcgcg ctggatcggg
      421 tcagtcaggt cgaccgcgat acgatgtaag gcgcagcgcg gttgctcgtt cgcgagttcg
      481 acttccttct cctcgagtat ggtcggcaga tctgcctcga accaacgcac gtcggcgggg
      541 aggggtaatc gataaggccg agtgtcaaag cccgcggcca agttgagtac gcaggtgcac
      601 ccatcctgaa ccgcagataa aatcaggtcg tccaaaatct tggtgcgcgt gacgatcggc
      661 caaccattgt ggctaccgtt gaatccacgc atgccacgcg cgattcgctg gcccttttct
      721 ccggccaagc gcgcggcata aggatcgtga aagagcgcat cgcgtcgctt ggtctcccac
      781 gcccgatagg ccgccaccca acgcgcggtg tcggatacgt cgtcgatctc ctgtctcgat
      841 gcttgagtcg tgctcatgat tcccgcgcgc agccaccgtc ggtggggcca aatcgaacgt
      901 tggtcgtcga ttgttcctgg attgctcctg aatattaccc ggcttaaatc agctgtcaat
      961 attagccggg taaaatattc agggccgcgt ccgacggccg gcgcgcttcg gggtgcaggc
     1021 gcccttcggg aagctacagc atcatgccgc ccgaaaactc gatccgctgc ccattcaacc
     1081 aatggcagcc cggagacagc agggcggcga ccgcaccgcc gatgtcgtct ggaaggcccg
     1141 ctcggccgag tgctgtctgc gaagcgatga ttcgattgag ctccgcattg tcgcgcacgg
     1201 cgccgccgcc gaagtcggtc tcgatcgcac ccggcgccag cgtgttgacc gtaattcgtc
     1261 gcgccccgag ctctttggcc atgtagcgtg tcaagacctc gatcgcgccc ttcatggctg
     1321 cgtaagccga gtagcccggg aaggtgaaac gcgcgaggcc gctcgacacg ttgagaatcc
     1381 gccccccgtc ctcgatccaa ggcacgagct gttgtgacag aaagaacggt cctttgaggt
     1441 gcaccttcac gagttcctcg aactgctgct cggtcgtctc ggtgaacttc gcatgcaaac
     1501 cggtgcccgc gttgttgacg agaaagtcga agcgttcacg ctgccacgtc tgccgaagcg
     1561 cttcgcgcac cccggtcacg aagctcggga aggtggcgct gtccgaaaca tcgagcggga
     1621 gcgccgccgc aagacgacct ttggcacgca gcgccgccac cacttggtcc gcctcggtgc
     1681 gtgccgaacg ataggtgacg atgctgtcca caccgtggtc ggcgaggtga agggccatac
     1741 tgcggccgag cccgcgactt ccaccagtaa tgagcgcgat acgaggacgt gatgaattgg
     1801 tcatgttgga acctcgagtc ggttggagtt cgatctcgtt tcggtcgaga acttagagtc
     1861 aggcgccgct cttagaaagc cttggcattc ggacagattg tttcgtattg ggaaacaatg
     1921 tctttggatc tggaaacgct gcgagtgttc gtgaaggtgg cggagttggg tagcttcacc
     1981 cgcgccggac agcatttgcg gctctcgaag tctcgcgttt cattgcgtgt tcaggagctc
     2041 gagacccggg tggggagtcg cctgttgcaa cggacgacgc gcgccgtgca gcttacgccc
     2101 gacggcgagc agttctgcga gcgcgcacgc aggctgctga atgatgcgga ggagctcgcc
     2161 gcgtttctgc aggcgccgag cgcgctacgt gggcgcgtac ggctggactt gccgatcgcg
     2221 ctcgctcgca acttggtgat cccgaggcta ccagagtttc tgaccgccca tcctcagctg
     2281 gagctcgagt tgagtaccac cgatcggcgc gtggacttgg tgcaggaagg gttcgattgt
     2341 gtagtccgga tcggcaatct tgccgattcg gggctggtcg cgcggcgctt gggtgagctc
     2401 gacctggtga attgcgcgag cccttcttac ctgttgaagc gcggtatccc tcgcagtctg
     2461 gccgacctgg cgaagcacca agtagtccac tactcactgc gtttcggtgc cgacgcgccg
     2521 tcgttcgagt atcacgatgg cgagcgttac cgagagttgc cgatggcgag tgtcatcacg
     2581 gtcaacaacg gagatgctta tcgtgacgca tgcttggccg gcttgggcat cactcagatc
     2641 cctagaatcg gggctcggtc cagtctcgcg tcgggggccc tcgtggagat cctgccggac
     2701 ttcgcggcgg ctcccatgcc gatctggttg gttcaagggc atacccgaaa tgttccgaag
     2761 cgggtgcgcg tggtaatgaa cttcttggcc cagctcatcg agcgagacta cggctgagct
     2821 tccggtgctc cgttcaccga gttcggcccc acgggactgg atgttgcggt gctggcagcg
     2881 catactcacg ccgtcgtgat cccgaccaac tttacccagg ctcggcattg ggcgcggggc
     2941 ctgctgctat ggtgcgtgct gtcttcttgc ggcgcgccgg cagctccgcg ctcgacgacg
     3001 cgcgtggggc gaacaccggt gaacgctgtg tcaacgtcgt ccagcgcgtt gatcgctccg
     3061 gcttcgggcg agagccagcc ggccgcgcga tggttcgaag atcgggccca ggccgcgttc
     3121 gagctcgccg agcaacgtgg tcagctcgtg ttcgcggatt tatgggcgcc gtggtgccac
     3181 acttgccttt cgatgcgcga gcacgtgctc gatgttcggc gcgtgccgtc tctcggtcga
     3241 gtggtgttgc tggccatcga taccgagcgc gcagagaatg cctccttcct ccaacagtat
     3301 ccggtcggag tctggcccac gttctacttg atcgatccgc gcacccgttc ggtgcgtgga
     3361 cgttggctcg gtgcggcgtc gcccgagcag ctcgatcgct tcatcgggga tggtgcccag
     3421 cctgggtccc aggccgcgat cctgttgagt gaggcggatg ccgcggcagc gaagcgagct
     3481 tggctgcagg ccgcgaagtt ctatcggggc gcgttggagt ccgcacccgc gggttggccc
     3541 cgtcgcggtg acgttctggt ctcgctggct tcctcgctgt acaagcagga aagcttcgag
     3601 gagtgcctgc agcttgctct cagcgcggtg cagtgggtgc cgccatcgat tagcgccgcg
     3661 gacttcgcgg cgacgacctt gggctgtgcc gagcgggcgg gcgaaggctc gaaggactcg
     3721 gcgcgtcgag ttcgacaagc tttggcgggg ttgctccaag ggctatgcgc gagtgcggcg
     3781 ccgggcgcga gcccggacga ccaagccgac gcatgcggca gcctgcgccg cgctcgcgaa
     3841 gcgctgggtg atcgcgaggg ggcgcggcaa gccgccgagc aggggctcgc cgtcatcgag
     3901 cgagcgacac tcggcgcaaa gccagactct caactcatct atgattggga acgcacttcc
     3961 aatctcgtct atctgggacg caagcgggag gctctcgagt tgctcatgca gcgagagcgg
     4021 gattgtccgc aaagctacaa tcccgcgcac tacttggcgc gcctgtatcg agacgcaggg
     4081 gagtgggaag caggcttggc cgcgatcgag cgagcgctcg cgaaagcata tggcccgaga
     4141 cgcgcagggc tagtgggcgt caaggtggat ctcctgcgcg gcgccgggcg cacgtcggaa
     4201 gctcgcgcta ctctggagca acagctgtcg gagtatcgcg ctctgcccga gggtcaaaaa
     4261 cagccggaag cggaggcaac ggtccaaaag cgactgagtg attggccact cccgcagctc
     4321 gagcactagc gcgttttcga ttcagcggcg ggcccccgcg acccgcgcaa cggtgggtag
     4381 gtagctcgca agccaagcgc tggccttctc ggtgcagtcg aacaagcgta ccggggctcc
     4441 gcgcgtatgc atgccaatca tgaggtttcc gacgatgcga cccagcagcg aattggtcac
     4501 gattgcgacc gcgctcacgg cgctagtcgc ttcgtcagag aggtaatgcg cgcggcactc
     4561 cggcgtctgt gccgtggcgg cttcgaagtg aatcagcaat ggccgtacca ctcccgcgca
     4621 gacgctcttg actgcctgca cgttctcgac ggcgtcatcc aaggtttgtt cgcggcgcgc
     4681 gagaggctgc aggtggacga ggccgtcgtc ctctccgagc cagacgcgcg ccgtgcgcgt
     4741 tgtaagttca gccatggacc gattttcgtc tcgagtcgac ctggggggac gaacgtaata
     4801 cggcccgccc acgccgttga tgaggctcgt gcggtactgc gctgcaaact ctacggatgt
     4861 tgtggtcgcc tgacgcacct acttccgggg gaccgagctt acaaccgcta gccgaccgag
     4921 cccccccgtc gatgaaccgc gccttcatca gtccctccga cgtacaatca atccgcgaga
     4981 tggccaaacc tgccgctgct gaagtcccga aaagcttcga ggatctccgg ctcggtattc
     5041 atgacgaaag gaccgtattg cacgacgggc tcgtcgatgg gctcaccgcc cagcaagagc
     5101 acttgtgcgt ccgagtccgc ctcgatgtga agtcgatcgc catcgtttgc gaataccgcg
     5161 aaatccattt cccgcgcggg agcgtcgttc aaacgaacgt tgccttgcat gatcaaagcc
     5221 gccaggttgt gcttcgacgg tatcgtaaag tctgcgcttc ctcgcgcggt tagagtcagg
     5281 tcgaagatgg atataggggt gaaggtcttc gccggaccgc gcgcgttgcc gtactcccca
     5341 gccaagatcc gcacagatcc cgcggcgtgc gggaggtcca cccgacccat atcggaagcc
     5401 aagatgccct ggtaccgtgg cggcgccatc ctgtgggccc gcggcaggtt gacccagagc
     5461 tgcgccatgt ggaagacgcc gccgcgctcc gcgaagttct gctcgtgata ctccttgtgg
     5521 agtactcccg aggcggcggt catccattgc gcgtcaccgg ggccaatcac gccgcctcca
     5581 ccggcgctgt catggtgggc cacgctgcct tgccaagcca aggtgacggt ctcgaagcct
     5641 cggtgagggt ggaccccgac gccccgcgcg cgtctcgtcg gcggatattc atacggtggg
     5701 tggtagtcga gtagtagaaa tgggctcagc tggcgtaccg catccggaat ctgcgagagg
     5761 taccccgcca cacgaaagcc atcacccacc cagtgcagtg gaggggcgga ataaatgccg
     5821 ctcaagccgc gagttttgat cgtgggccga gcgccggccg gcgatggaac accgagcgcc
     5881 ggcgctgaaa cctgagttac ggaggagttg ggacgggtca tgttctgcct cggaatgcac
     5941 tgtggtcacg cgacccgagc cgcacaacat gcgcccccgg cacacactga tgtcgagaat
     6001 cgaacaatcc ccaaagagag gccggaccgc tcaagaggcc gcgcgtacgc taggtgattc
     6061 ccagttggtg cccttctcag cggccctgcc gtgggaaagc gcgaaggccc catctcccaa
     6121 cagcagctgt gtgaacaggg caatcagaag aaacgcgaca tactcccagc cgccgcccga
     6181 gttggtgaac atccagccat tggcgagatg ggggacgagt gccccgagca tcacggggat
     6241 cagcgcaagc gcggcgacac gtgagtacaa accgagcagc aggctcaccc cgccgatgag
     6301 ctccaacgcg aaaacggggt aggcgctcca gcccggaaag ccatgcgctt cgaagaacgc
     6361 gcgcgtcccc gggagcgtga agatagtgag tttggcgtag gcatgcgcga ggaacacggc
     6421 gccgagcccg agccgcagca gggtgggcgc gtactgcgcg tgagagtgag cgaatcgatc
     6481 gaccgtccga agagtagttc gtgtcatagg cagtactttg ggcccgagcc ttcgcggtcg
     6541 gaaggtagtg gcgtacgaac gcagcgttcg ctggcgcgaa caccaatcga ggtgcctctg
     6601 cccgattcgt tgcgctcgac tctaagctct cgggacgggg gcttgccagt ctgggccttg
     6661 aaaggcggct tcgaaggcgg ctatcaactc tgcaaccagc tttggcggcg tgggtcgccc
     6721 ggagcgaacg gcgtagatcc ccattgggcg tggaccgaac gagtccaaca cggtctcgag
     6781 cgttcccgct cgcagcgcgt ccgcgatcat gaagcgcggt aggatcgcga gccctgcacc
     6841 ggcgatcgct gcctcgcgca acatggtgcc gttggtggtc tcgaacgaac ccgtcaattc
     6901 caaaggaacg cggcccccct cactgtaaag gcgccattcg tgatccggcc gcagataggc
     6961 gtacctcatg cagttgtggc gaaccaagtc ttcggggcgc ttggggcggc catgttcggc
     7021 aaagtagctc ggtgagccgc agacgtgaat ggtagtccaa cccaaacggc gcgcgagcag
     7081 gctcgagtct ttcaacttgg tgatgcgcag ggccagatcg atgcgttctt caatcaggtc
     7141 taccaatcga tcattcagaa gaagttcaat gcgggaccga ggatgtgcac tcaagaactc
     7201 ggcgatcgga cgtgccaaga agagctgccc caaactgacg ggtgcgctga gccttactac
     7261 gcccccggat gccgccgaac tatcgcgggt cgcctgacgg ccggcctcga ccatctgacg
     7321 ggcgtgtgag tagactctgg tgccggcatg agtgggagtg acgttgcggg tggtgcgcaa
     7381 cagcagctgc tcaccgagcc gcgtctctaa ggccgagacc ttctcgctca cgacggactt
     7441 cgagacaccc agcttggccg ccgccttggt gaacgagccg cattcgacca cccgcaccaa
     7501 gtacagcaag gcgagcaggt catcgagcag aggatccggc atgagggagc ttcggtacga
     7561 attcgggcct gccgcaagct cgaaatcagc gtcagatcga gctgccttcg gcggccatct
     7621 ggagattcac tcgaatcatc gacgcgaagg ctgcgccgac gatcgagaat tgctaacagg
     7681 tcaagctcga tggaactcac tcacattcgc ctgctcgtat acgacttcga gcaaaccttc
     7741 aagttctacc gggatttgat cgggctgaag ccgcaggcgg acacgccgca gggcccctac
     7801 gcgaagttca gtttcgcaga aggcgcagcg gcaatttcct tgcagcagcg cagtcacttc
     7861 atcgaaaata gcgtgccgct gcgcgaacgt tcgagtgggc tggcggccgg cactgacaat
     7921 gctttgattg cgatccgggt tgcgagcgtc gcagaagagg tggagcgcct gcggcagagt
     7981 ggggctgaag tgactggtcc gcgcgatctt ttcggccgaa tgacgtgcgc gtggctccgt
     8041 gatcccgagg gcaatctgat cgagcttcag gagtgggtca acgccggagc tcgaaagtcg
     8101 tgagcgggta aggcgccccg ttgacgagca gttctaggcg atgcgtgccc gggtagtgct
     8161 gtcgcgtggt catgggggcg aaagagatct tgccgcggag ctcggtccgg ctcttcggtt
     8221 ccagcagcag ccgcttgagc ttgaagacct tcgggcgagt atggccttgg aacttcacga
     8281 agtgaaccac gtagtctacg agcaggtcct gctcgcgcgt gctgatgctg cgcagagcta
     8341 cctcgaaacg tacagtttct cccagcttgg ggcgcggagg tgtcagtcgc accgcctcta
     8401 tctcgacgcg ggcgcgcttt cccgccccga ccaacgcgag cgcgcgcggg gccgcctgct
     8461 tgatcaagga tcgcaaacca tgacggatga tccaggctcg gctcgtcgaa gcaccggcat
     8521 tccagcgctc acacgtatcg agtaccaagt ccggatggtc tttggcgatg tcgttcaagt
     8581 tgttggcaac tgatcgctgc acgtaacgct cggaatcgtc tttgagcaac tctaacaact
     8641 cgagtaccgg gcgaggatcc tgctggaagg cgcgcaaccg tggcgcccaa ggcaaacggg
     8701 gacgcgtgcc ttccgaaact agcctgcgca cgtgaacgtt cggatcgagc gcccattgtt
     8761 ggagccgggc atacgtcgcc tccggatgac ggacgagaaa agcgcgtatg ctggattcag
     8821 ccgagaatcg cttggtgagc tcgcgctgta gggccattgc tggctcgaag tggtctagcc
     8881 cgtacttctg cacgtaaaaa acgtgaggta gatattggag tggagatagg cccaaagact
     8941 ccgtatcggc gagctcgggt cggagcgaac ggatgagcac ctcggccgct ctcgggaatg
     9001 gctgcggcaa gtattggtgc aacgcctctg ctacgtgcca agcacgtggg gtgagctcga
     9061 gttcttccag cccttgccgg ctcgctcgtg tgaatctctt cgcgtcgaat tggggatgcg
     9121 cggcctggag ctcgctcgcg agctgctcga cgaaacgccc gtccaggata ttcttgagct
     9181 tggccgcagg agttggttgg gatttggcca tcgagtgctt tccggtagcg cagcgagaag
     9241 gcgctacgac ccgaggccgc ttgactagcc cccaatggtg acagaatctg tcaccattgt
     9301 gtcgcgtgcc gaacgattgt tgcagctctc cgatctgctc cgaggaaccg aggggagtac
     9361 ggtggctcag cttgcgaatc aactcggagt gagccgccgc accctgctcc gagacctcgc
     9421 ctccttacgc gatcggggca tgccaatctc gagtgatgtc gggcgcggcg gcggcgttcg
     9481 gctggaaggt gaccggggcc tgacggcttt gcacttgtcc gtatcggagg tggtcgcaat
     9541 ctggctcgcc gcgcgcctat ctcgtgaggc gagcgagttg ccgtgggcgg aggcggccaa
     9601 ttcgggcatg gccaaattgc tggccagctt gcccaaaccg aagtctcggg cgcttcgcgc
     9661 actgtgtcgg cgcgtgatcg ttgggcggcc tgcttccgcc aatgtgcgcg ccgggatcgg
     9721 ggcgcctccg ccggagctat tgcgcctctt cgaggaggca ttctcggcgg ggctcgggct
     9781 ggcttttcac tatacggatc gagagcagaa ggtgagtatc cgccgggtgg agccgcatgg
     9841 gctactcgtc gaaaccccag cctggtatgt gctcgcgaga gacatcgaca aagccgaacc
     9901 gcgcatgttt cgtatggatc ggattgcgcg ggcccggatc ctgccggacg tagaattcaa
     9961 gccagatttg cgcgtcatcc aggcgcaatt tgcagacctg agcggttggc atcctcttgc
    10021 ggggcattgg ctgacatgag ggctgcgaag cggtgggcga ccgctcagcg ctggatcatg
    10081 ttagggcggg gaaactgacg gagcagacgc ttggccaacc tacgatctcc gcgcagctcg
    10141 atgcctccga gcgccgtcga gcggccaccg aacaccaccg ccctcagcgt cgccggatcg
    10201 ctcaagattt gggcgttcgg gtgtcctgga gtgcctcggg tgatcgtgag atttggtccc
    10261 tccacctcca ggatgaagct gtcaccactc aaccgcagct cgaagacata gcggtccgtc
    10321 catgactcat tcggctcgaa cgtggtctcc agcgcgatta tgagcgcatc gacgctcagt
    10381 tcaccggcgg gcctgaccgg cgattgcgcg ccccagcggc ccaaagcgac cagaatgggg
    10441 tggagctcgc gaccccagtc gctcagctcg tacgtgcccg cggtatcggc gcgcttctgc
    10501 acgacaccgg ccgcctccag ttctcgtaaa cgctgagaca gaacgttggg gctcgcgcct
    10561 ctcaagccgg ttcgaagatc ggtgaagcgc ttgggtccaa acacgagctc tcgtacgatg
    10621 agcaggcccc agcgctctcc gactacgtcg agtgcgcggg caattccgca agggtcttcg
    10681 tagcttcgca agctggtcat gggagccgat catacttgga cttgcagaaa tgaacaaaat
    10741 agtcctagct taggactaag ctccagggca gagcgagccc gcaagattgg gctctggcga
    10801 aggaaacagg atcatgcgca gagtacgtgt cttcgagaat gtgtctttgg acggttactt
    10861 ctgtgacgag cgaggtgaca tgagctgggc gcaccgctcc gacccagaat ggaacacctt
    10921 cgtggcggag aacgcgagtg gtgagagcca gctagtgttc ggccgcgtca catatcagat
    10981 gatggccggc tactggccaa cccccatggc ctttcaacag aatccccgcg tggcggagcg
    11041 gatgtccgcg accaagaagt acgtttgttc tcgctccctg agcgcggccg acttgggctg
    11101 gcaaaactcc gagctcgtgt ccgatctcgt ttccggcgta caagcgctca aacgagaggc
    11161 ggggccggac ctggtgatct tggggagcgg aagtctggtc gcgcagcttt ccgaagcggg
    11221 tctcgtcgat gaataccaag tcgtcttgca gcccatcgtg ctcgggaagg ggaagtcctt
    11281 gttctcgggc gtgacccatc gcatcccgct tacgctgaag cgcagccggt gcttcgggaa
    11341 tggcaatgcc gtgctctggt acgaacccag cgtcggataa cgtgcgcggc agaccggatg
    11401 cgcacgggcc gtgcgcatca ccggaacgat agattgcgag cggcgcgccc tagctcgagt
    11461 cgaccgccac gtgctacgtg tgtgattcag gtgtgcggaa aatgcggcaa gtggacccat
    11521 tgcttcgttg gtgcgaaaac ccattcttcc tactcggctt gatgcccacg gcgtccgaac
    11581 gcgacatcga acgcgagggg cgcaagcttc taggtcagct cgaactcggc ctacgctcgg
    11641 cacgttgggc gaggactcca cttggcgagg tggagcgcac acccgattcc atccgctcgg
    11701 caatagcgga gctgcgcgat cctcagaagc gccgagtcca cgccatttgg gctgcactcc
    11761 ccatccactc gccgggggtt tgggggccta ccgattccga aggccacgct ccgcctaccg
    11821 gcgttaccga ggagccgttc ctagaggcca tgtctgcggc gggatggccc gggttatgag
    11881 cggctcgatc aatccaggga agcacgcctg cttggcggag agagcgggtt gagcgatgtt
    11941 tccagtcggt gccatcgttg ccgccctttt cgcggctcgg cgttctagtc cggcgatgaa
    12001 gccgagccgg gcgctggtac tcgggtcgaa gtatgtggtg cagtggcaag gcgaagaaaa
    12061 gcacatgacg tggtctctcg cgttggtgtt gatcatgcct ctggcgctcg tggcg
//
LOCUS       DASTKU010000298        10665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1173527, whole genome shotgun sequence.
ACCESSION   DASTKU010000298 DASTKU010000000
VERSION     DASTKU010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173527"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="VFQ61_10725"
     CDS             complement(<1..1418)
                     /locus_tag="VFQ61_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9954970.1"
                     /translation="MPAAPIGAANAPPDLLALAEVGRELTSGGVLGDAFTRAMRSLER
                     RLGVRRATLCVADPDRRTLDIEATYGLERGHFRPRFGSGVAGRVADSGRPIVVPTLRH
                     EPMALSELTDPQAWSEGDWTLLCVPLSMGGRNVGAFSAYFARGDSQENGGRLGVLEIV
                     ASLLGQALRAADLERTKLPEERSSDEPAGRSERALFEYANMIGTSAAMRQVYEQVGQV
                     ARTNATALIRGESGTGKELVAHAIHNNSLRARQPFVKVNCAALPETLFESELFGHERG
                     AFTGAHARRKGRFELAQGGTLFLDEIGELSPTTQAKLLRVLQSREFERLGGSETLRTD
                     VRIIAATNKEMERAVANGTFREDLYYRLNVFTISLPALRERRADTPALAEYFLTKYAA
                     EHRRKIGRISSAALELLTGYTWPGNVRELENVIERAVVVCDGFVIQEHHLPEPVRVVT
                     RNAPTEKPTLAMAVARLERQMI"
     gene            1589..2308
                     /locus_tag="VFQ61_10730"
     CDS             1589..2308
                     /locus_tag="VFQ61_10730"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9954971.1"
                     /translation="MERALSKLGAASRSVAHRAILNGQVTVGGRICRDPGLRVSPERD
                     VIALDGLRLRRAKRLVLVVHKQRGTLTARRGSTDRPTVFDVIPPELGELHAVGRLDFA
                     TSGLLLLTNDTRLSAFLTDPLNGIPRVYRVSVRGEFESARVAQLLAGVEDAGERLFAE
                     HAELSKASGRESHLRVTLSEGKNREVRRLFAALGHEVTRLCRVAYGGIELGELAPGKH
                     RVLSEPELKRAFPTYPGWRSR"
     gene            complement(2452..3168)
                     /gene="phoU"
                     /locus_tag="VFQ61_10735"
     CDS             complement(2452..3168)
                     /gene="phoU"
                     /locus_tag="VFQ61_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET9954972.1"
                     /translation="MPLSKMAQHKHIDLEFEGELERVRERVLLMGAHVEEMLGRAMRA
                     FEENNAGLARVTIDSDAVVDQLEISIDEACLQILARRQPVASDLRFVTTTLKLVTDLE
                     RIGDLCVNLCERVIECAEEPPAPRGSSPIPRIATAAHAMLHDALDAFVAGDLHKAQDV
                     LGRDRLVDAYYAQLFPELVARMMADPSVVFRSTRLLSIGKYLERIADHATNIAEMVVF
                     MVKGEDVRHVDPSNPPPATH"
     gene            complement(3187..4077)
                     /gene="pstB"
                     /locus_tag="VFQ61_10740"
     CDS             complement(3187..4077)
                     /gene="pstB"
                     /locus_tag="VFQ61_10740"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HET9954973.1"
                     /translation="MQAPIQLDPGAAFRKSRASESSRPDASGAPPAERRSRREDVPLD
                     ALPSKMRAQGLHAHFGSTEVLKGIDLTIAERHVTAVIGPSGCGKSTFIRCLNRMHEVV
                     PGARATGRVLLDSEDIYGARVDSVRVRRRVGMVFQKPNPFPTMSIRDNVLAGLRLNGL
                     RLKDADTVVEKALRQVALWDEVKDRLLRAGTSLSGGQQQRLCIARSLALSPEVLLMDE
                     PCSALDPIATARVEELIHELRTSYTIVIVTHNMQQAARVADFTAFFYMGKLVEHDRTD
                     VIFTRPKERQTEDYITGRFG"
     gene            complement(4151..4993)
                     /gene="pstA"
                     /locus_tag="VFQ61_10745"
     CDS             complement(4151..4993)
                     /gene="pstA"
                     /locus_tag="VFQ61_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET9954974.1"
                     /translation="MNDYTRRKLLDRLVRGACVLATMLALVPLGSVLYYVFIRGIGGL
                     SWEFFTELPKPVGETGGGMANAIVGTLKLVGLAGLFGIPPGVLAGIYLAEFPRARLAH
                     AVRFCADVLSGIPSITVGLFVYALVVMTTKRFSAFAGGLALAVLMLPTVTRTTEELLK
                     LVPEALREAALGLGVPKWRATLRVMLRTALPGILVGVMLAVARVAGETAPLLFTSFNN
                     RYWADSLNQPTASLPVNIFTYAVSPYEDWHRQAWAAALVLLVIVLVLDVASRLLTRDT
                     RGSR"
     gene            complement(5006..5992)
                     /gene="pstC"
                     /locus_tag="VFQ61_10750"
     CDS             complement(5006..5992)
                     /gene="pstC"
                     /locus_tag="VFQ61_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET9954975.1"
                     /translation="MAQARDASLYSPRSSWLGRFGLRGNAGDLLFRGVTMLFAIGVVL
                     VLGATAFEMLRASELAIRTFGFEFVTGTTWDPVRQVFGALPFIFGTVVSSLLALVIAV
                     PISFGIAIYLSELAPGWLRQPVSFLIELLAAVPSVVYGLWGIFALSPWLRETVQPALQ
                     AMFGFVPLFQGPRYGFGMLAAGIILAIMIVPTVSSVGREVLRAVPANLREGALALGAT
                     RWEAVRFAVLPYASSGLVGAVILGLGRALGETMAITMVIGNRPEISASLFAPGHTMAS
                     VIANEFTEATDDLYLSALAQMGLLLFGVTVLLNVLARLLVWRVGRGAASAQT"
     gene            complement(6008..7138)
                     /gene="pstS"
                     /locus_tag="VFQ61_10755"
     CDS             complement(6008..7138)
                     /gene="pstS"
                     /locus_tag="VFQ61_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET9954976.1"
                     /translation="MTTRRALLLGSLAASAIWLATACNKSSPPDERASKDGEASRKQD
                     AAKPAAAKGITLNGAGATFPFPLYSKWMSEYNKANPDVRINYQSIGSGGGIRQVVAGT
                     VDFGATDAPMKEEESKGAPGKLVHIPATLGSVVVAYNLEGVSGHLNLTSEALSGIFLG
                     TVKKWNDKKISAQNPGVKLPNQDIAVVFRTDGSGTTAVFTEYLSTVNPAFKEKVGAGK
                     SVKWPAGLGAKGNEGVTGQVKTTPGAIGYMELAYATQSKLPTAAIQNKASKFVAPSAA
                     GATAAAESVEMSEALHVSLADAAGEAAYPIASYTYLLVYEDSKDSEKGQALAKFLWWA
                     LNDGQRFAADLDYAPLPDKVQAKVRERLKTLSSGGKRLLDGV"
     gene            complement(7250..8551)
                     /locus_tag="VFQ61_10760"
     CDS             complement(7250..8551)
                     /locus_tag="VFQ61_10760"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET9954977.1"
                     /translation="MHTLQRSLLGLALIAVASPVLAQGVPEPAVPAPAPTPPPVAPGL
                     EPPAPATAPSAPPELAPNQALAAPPATFAVAPAASAPAAIPAAVEEPKPTLPDRLAQG
                     KDGGFLQFSGLLQFWAFYSHDSENGTAPASHTMTGRLRRAEIKLKGEIIPKVFGYTLM
                     LDGAKAFSGGNATLTVVDTSKTTVGTTTTTVPRSDTPLQDFYITFMTDYADFSVGQFK
                     TPISLEGVTSAARLMLPERSRVATTFGDRRDIGLRIDKKLGNYFYYYAGVFNGNGQSP
                     TDNDNDKDVALRLETYPIEGLTLAGMGYTTVGDRVTVRDRLEADLRYDAHGVLAQAEY
                     IHAWDGVRNNGHRTEGHGAYGALGYTFDKRVQPVVRVGFVNPDIRSSKEANSFKGIYE
                     GGVNYYIKGHEARVGLAIAVFDPKTTGPTHVEGTLAVQAAY"
     gene            complement(8718..10118)
                     /locus_tag="VFQ61_10765"
     CDS             complement(8718..10118)
                     /locus_tag="VFQ61_10765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9954978.1"
                     /translation="MKRGGRLQRFALSLAVNGVCLGIPLWLALDGSAQGEAERQRLGL
                     AGLVGILASSTFFASWAERERSRRWQALDVTAGHLAKLRWDSDEVVASARILTHSERE
                     IERGFDALARVLVNLREQRDRLGGILAAMEEGVLLLDAQGRIALTNPALREMLLIGPD
                     AVGRTLLEVIRRTELKELFDRAEATKEPVSCELEAGTLKPRRLLVRVASVAGEPAQHF
                     GVFIDVTEVRKLESMRRDFVANVSHELRTPVTAIRSAAETLQVGLPEDPAVLRQFIGI
                     IDRNAVRLHDLVEDVLSLSHIESQKLRLNMEPLELRSVCSQVSSLFRERAERKQIRLE
                     NTISSAGARVQADRRALEHVLGNLVDNAVKYCAAGATVSVEARDVGESFELSVRDDGP
                     GIEARHLPRIFERFYRVDAGRSRDVGGTGLGLSIVKHLVEAMGGNVKVESEPGRGTRF
                     FFTLRKAELEVRQAVA"
     gene            complement(10115..>10665)
                     /locus_tag="VFQ61_10770"
     CDS             complement(10115..>10665)
                     /locus_tag="VFQ61_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9954979.1"
                     /translation="DLMLPDISGTEVCKTLKEGATTKSIPVLMVTARGEEIDRVVGFE
                     IGADDYVVKPFSVRELLLRVQAVLRRGKPESEGQDSHIEFGRLRIDRGAHRVWVEGQE
                     IELTALEFKLLVTLYERRNRVQTRSALLDDVWGIQADITTRTVDTHVKRLREKLEAAR
                     DYVETVRGVGYRFVASPEEAAT"
BASE COUNT         2021 a         3541 c         3272 g         1831 t
ORIGIN      
        1 tcaatcattt gccgttccag gcgggccacg gccatggcga gcgtgggttt ctcggtgggc
       61 gcattgcgcg tcaccacacg cacgggctcc gggaggtgat gctcttgaat cacgaagccg
      121 tcgcacacga ccaccgcgcg ctcgatcacg ttctcgagct cacgcacgtt tccgggccac
      181 gtgtagccgg tcaacaactc cagcgctgcg ctcgagatcc ggccaatctt gcgccgatgc
      241 tcggctgcat acttggtcag gaagtactcg gccaaggccg gggtgtccgc gcgccgctcg
      301 cgcaacgcgg gcagagatat cgtgaagacg ttcaggcggt agtagaggtc ttcgcggaac
      361 gtcccgttgg caacggcgcg ctccatctct ttgttggtgg ccgcgatgat ccgcacgtcg
      421 gtgcgcagcg tctcactgcc acccaatcgt tcgaactccc gcgactgtag aacacgcaac
      481 agcttcgcct gcgtagtagg cgagagctcg ccgatttcgt ccagaaacag cgtgccaccc
      541 tgagcaagct cgaagcgccc cttgcgccgt gcgtgggcgc cagtgaacgc cccgcgctcg
      601 tgcccgaaca actcggactc gaacaacgtc tccggcagcg ccgcgcagtt gaccttgacg
      661 aaaggctgtc gcgcgcgaag cgagttattg tgaatcgcgt gtgcgaccag ctccttgccc
      721 gtgccggact ctccgcgaat cagcgccgtc gcgttggtgc gcgcgacttg gcccacctgc
      781 tcgtagacct gccgcatggc ggcgctggtc ccgatcatgt tcgcgtactc gaacagcgct
      841 cgctcggagc gccccgcagg ctcgtcgctg ctacgttcct cgggaagctt ggtgcgctct
      901 aaatctgccg cacgcaaggc ctgcccgagc agcgaagcca caatctcgag cacccccagt
      961 cgaccaccgt tctcctgcga atcgccgcgg gcaaagtatg ccgaaaatgc accgacattc
     1021 cggccgccca tcgacaaagg cacacatagc aacgtccagt caccctcgct ccaggcctga
     1081 gggtccgtaa gctcggacag cgccatcggc tcgtggcgca gcgtgggaac cacgatcggt
     1141 cgcccgctgt ccgccacgcg gccagccaca ccactgccga aacgcggccg aaaatggcct
     1201 cgttcgagac cgtaggtcgc ctcgatatcc aaggtacgcc ggtcgggatc tgcgacgcac
     1261 agggttgcgc ggcgcacacc gagccgccgt tcgagtgagc gcatcgcgcg cgtgaacgca
     1321 tcgcccaaaa ccccgccgga ggtgagctcc cgccctacct ccgccaacgc caagagatct
     1381 ggtggagcat tggctgcgcc gatcggagca gccggcatag aagaatcgaa tcgacggaga
     1441 ggcacgagcg agacggctct catttatcgg gtttatgctg tccgagtaaa ccttgccggc
     1501 acgtttccga cagatgaact ctgatgcaac tcgcccgcga acgagaaaca cctcagaaac
     1561 agaacccact cgctctggaa ctgtttcctt ggaacgcgcg ctgtcgaagt tgggtgccgc
     1621 gagccgtagc gtcgcgcacc gagcgattct gaatgggcaa gtaacggtag ggggaaggat
     1681 atgtcgcgat ccgggacttc gggtcagccc cgaacgcgac gtgattgctc tcgatggtct
     1741 gcgcttgcgc cgcgccaagc ggctagtgct cgtcgtgcac aagcaacgtg gcacactcac
     1801 cgcgcgtcga ggaagcacgg atcggccgac cgtgttcgat gtcatccccc cggagttagg
     1861 agagctccac gccgtgggcc gcttggattt tgcgaccagc gggctgctcc tcttgacgaa
     1921 cgacacgcgc ctatccgcgt tcttgaccga tcccctgaac ggcatccccc gtgtgtatcg
     1981 cgtgagcgtg cgcggtgaat tcgaatccgc gcgcgttgct cagctcttgg ctggcgtcga
     2041 ggacgccgga gagcgcttgt tcgcggagca cgcggagctc tcgaaggcga gtggccgaga
     2101 atcacacctc agagtgacat tgagtgaggg caagaaccgg gaggttcgcc gccttttcgc
     2161 ggcgctgggc cacgaagtca cccgtttgtg tcgtgtcgca tacggcggca tcgagctcgg
     2221 tgaactcgcg cccggcaaac accgcgtgct ctccgaacca gagctgaaac gagccttccc
     2281 gacctacccg ggctggcgtt ctcggtgaat ggaggccgcg ccagcacggg cgcgaagcct
     2341 ggccgtgccc ctaccagcgc agcctaaacc cccgctctac ggacacacac cgcgccggcc
     2401 ccccaagtcg acgcgcatcg tcgaggagcg cgcgagccgc ggtccactcc gtcaatgcgt
     2461 agcgggcggc ggattggagg ggtccacgtg gcgcacgtct tcacccttga ccatgaacac
     2521 gaccatctcc gcgatgttgg tcgcgtgatc tgcgatgcgc tcgaggtact ttccaatcga
     2581 gagcaggcgc gtcgagcgga acacgacgct cgggtctgcc atcatccgcg ccaccagctc
     2641 ggggaagagc tgcgcgtaat aagcgtcgac caggcggtct cgtccgagca cgtcttgggc
     2701 cttgtgcaag tcaccggcca cgaacgcgtc gagcgcatcg tgcagcatgg cgtgtgcggc
     2761 cgtcgcgatg cgtgggatcg gtgaggagcc ccgcggagcg ggcggctcct ccgcacactc
     2821 gatcacgcgc tcacacaggt tcacgcatag atccccgatt cgctccagat ccgtgacgag
     2881 cttcaaggtc gtggtcacga agcgcagatc ggaggccacc ggttgtctcc gcgccaagat
     2941 ctgcagacac gcctcgtcga tgctgatctc gagctgatcc accaccgcgt cggagtcgat
     3001 cgtgacgcgc gccaggcccg cgttgttttc ttcgaaggca cgcatcgcgc ggcccagcat
     3061 ctcctcgacg tgcgcgccca tcaacagcac gcgctcgcgc actcgctcga gctccccttc
     3121 gaactccaga tcgatgtgtt tgtgttgcgc catcttgctg agtggcatgc cgggttgcct
     3181 cgcctttcaa ccgaaccggc cggtgatgta gtcctcggtc tgtcgttctt tcgggcgcgt
     3241 gaagatcacg tccgtgcggt cgtgctcgac caacttgccc atgtaaaaga acgccgtgaa
     3301 gtccgccacg cgcgcggctt gctgcatgtt gtgcgtgacg atcacgatcg tgtagctggt
     3361 gcgcagctcg tgaatcagct cctccacgcg cgcggtcgcg atcgggtcca gcgcggagca
     3421 cggctcatcc atcagcagca cttccggcga aagggccaaa gagcgggcga tgcacaaacg
     3481 ctgctgttgc ccgccggata ggctggtgcc cgcgcgcagc aaccggtcct tcacctcgtc
     3541 ccagagcgct acttgacgca gagccttctc gaccacggtg tcggcgtcct tgagccggag
     3601 gccattcagc cgcaagccgg ccagcacgtt gtcgcgaatg gacatcgtgg gaaacgggtt
     3661 cggcttttgg aacaccatcc cgacgcgacg acgaactcgc accgagtcga ctcgcgcccc
     3721 ataaatatcc tccgaatcca acagaacgcg gcccgtggcg cgcgcgccgg gtaccacctc
     3781 gtgcatccga ttgagacagc gaatgaaggt cgatttgcca cagccagagg gaccgatgac
     3841 cgcggtgacg tggcgctcgg cgatcgtcaa gtcgatgcct ttgagaacct ccgtactgcc
     3901 gaaatgagca tgcagccctt gtgcgcgcat cttgctgggc agcgcgtcta ggggcacgtc
     3961 ttcacgccgg ctccggcgct ctgcgggggg agctcccgaa gcgtcggggc gcgacgactc
     4021 cgaagctctc gattttctga atgccgcacc cgggtcgagc tgaattggag cttgcatggg
     4081 acttctccga agctgaaagt cgttgactga acggcagggg cctgagcgtg attggcgagt
     4141 gagcgtctcg tcagcgcgat ccacgcgtgt cacgcgtcaa gagccgtgag gcaacatcga
     4201 gcacgagtac gatcacgagc aagacgagcg ccgcggccca agcctgacga tgccagtctt
     4261 catacggaga gacggcgtag gtgaagatgt ttaccggcaa agaagccgtg ggttgattca
     4321 gtgagtctgc ccaatagcgg ttgttgaacg acgtgaagag caagggcgcc gtttccccgg
     4381 ccacccgcgc caccgccagc atgacgccca ccagaatgcc gggcagagcc gtgcgcagca
     4441 ttacccgcaa cgtcgcccgc catttgggaa cgccgagtcc gagcgcggcc tcccgcagcg
     4501 cctccggcac gagcttgagc agctcctccg tggtacgcgt caccgtgggc aacatcaaca
     4561 cggcgagcgc gaggccaccg gcaaaagcag agaagcgctt ggtggtcatc accacgagcg
     4621 cgtagacgaa caagcccacc gtaatagagg ggatccccga caatacgtcc gcgcaaaagc
     4681 gcaccgcgtg ggccagacga gcccgtggaa actccgccaa gtagatgccc gccaacaccc
     4741 cgggagggat tccgaacagg ccagccaggc ccaccaattt gagcgtacca actatggcgt
     4801 tggccatccc gccgccggtc tcgcctacgg gcttgggcag ctcggtgaag aactcccaac
     4861 tcaagccgcc aatgccacgg atgaagacgt aatacagcac gctaccgagc ggcactaggg
     4921 ccagcatcgt ggccagcacg caggcgcccc taaccaggcg atcgaggagc tttcttcgcg
     4981 tgtagtcgtt catgtcgaag gcatctcagg tttgcgcaga cgccgcgccg cggcccacgc
     5041 gccacacgag cagacgcgcc agtacattga gaagcaccgt gacgccgaac aacaacagtc
     5101 ccatctgcgc gagcgcagag agatacaaat cgtccgtcgc ctccgtgaac tcgttggcga
     5161 tcacgctcgc catggtgtga ccgggcgcaa acaacgaggc actgatctcc ggccgattgc
     5221 cgatcaccat cgtgattgcc atggtttctc cgagcgcccg cccgagcccc aagatcaccg
     5281 cgcccacgag cccagagctg gcatacggca acacggcgaa acgcacggct tcccagcgcg
     5341 tcgcgccgag cgcgagcgcc ccttccctca agttcgcggg aaccgcgcgc agcacctccc
     5401 gacccaccga agacacggtg ggaacgatca tgatcgcgag gatgatgccc gccgccaaca
     5461 taccgaaccc gtagcgcggg ccctgaaata ggggcacgaa gccgaacatg gcctgcaagg
     5521 cgggttggac ggtctcgcgc aaccacggcg acagcgcgaa gatcccccag agcccgtaga
     5581 cgacgctggg cacggccgcc aggagctcga tcaggaagct gaccggctga cgcaaccagc
     5641 ccggcgccag ctcggacaag tagatcgcga tgccgaacga aatgggtacg gcgatcacga
     5701 gcgcgagcag tgatgacacg accgtcccga agatgaacgg caaagcgccg aacacttgcc
     5761 gcacgggatc ccacgtcgtg cccgtcacga attcgaagcc aaacgtgcga atcgcgagct
     5821 cggatgcgcg cagcatctcg aacgcggtgg ccccgagcac caacaccacg ccgatcgcga
     5881 aaagcatggt gacgccccgg aacagcaaat cccccgcgtt acctcgcagg ccaaagcggc
     5941 cgagccagga ggagcgcggt gagtaaagcg aggcgtcgcg agcttgtgcc atgcgcgggc
     6001 tcgctgacta caccccgtcg agcaggcgct tgccgcccga gctcagggtc ttgagccgtt
     6061 ccctgacctt cgcctgcacc ttgtccggca gcggcgcata atcgaggtct gccgcgaagc
     6121 gctggccgtc attcaaagcc caccacaaga acttcgcgag cgcctggccc ttctcggagt
     6181 ccttgctgtc ctcgtacacc agcaagtacg tgtacgaagc aatcgggtaa gcagcctccc
     6241 cggcggcgtc cgcgagagac acgtgaagag cctcggacat ctccacactt tcggcggcag
     6301 cggtagctcc ggcagcggag ggtgccacga acttggacgc cttgttctga atggctgccg
     6361 tcggcagctt gctctgcgtc gcgtacgcaa gctccatgta accaatggca ccgggcgtgg
     6421 tcttcacttg tccggtgacg ccttcattgc ccttggcgcc cagcccggca ggccatttga
     6481 cgctcttgcc ggcacccacc ttctctttga aggcgggatt cacggtcgat agatactcgg
     6541 taaacacggc tgtagtgccg ctcccatccg tgcggaacac taccgcgatg tcttgattcg
     6601 gcagcttgac gccagggttc tgcgcggaga tcttcttgtc gttccacttc ttgacggtgc
     6661 ccaagaagat tcccgacagc gcctctgaag tcaggttcag gtgcccagac acgccctcca
     6721 agttgtaggc gacgaccacc gagccgagcg tcgcggggat gtgcactagc ttgcccggtg
     6781 cacctttgct ctcctcctcc ttcatcggcg cgtcggtggc cccgaagtcc accgtgccgg
     6841 cgacgacttg ccgaatgcca ccgccggacc caatcgactg gtagttgatg cggacgtcgg
     6901 ggttggcttt gttgtactcc gacatccact tggaatagag cgggaacggg aaggttgcgc
     6961 cggccccatt caaggtgatg cctttcgccg ccgccggctt cgcggcgtct tgtttgcggc
     7021 tagcttcgcc gtccttgctg gctcgttcgt ccggaggaga agacttgttg caagccgtcg
     7081 cgagccaaat agcgctcgcg gccaaactac caagcaggag tgcacgtcgg gtggtcatgt
     7141 cttgcgtagg cctaacagcg ccgcacgcgg agcgcggggc acggtcgcaa caggtgcgtg
     7201 acacttggat gaccgaaccc agcgatcccg ctcgactgac tagcgcggct cagtatgcgg
     7261 cttgaacggc gagcgtgccc tcgacgtgag tgggccccgt cgtcttcgga tcgaagacgg
     7321 caatcgcgag accgacgcgc gcctcgtgac ccttgatata gtagttcacg ccaccttcgt
     7381 agatgccctt gaagctgttg gcttccttcg agcttcggat atccgggttc acgaagccga
     7441 cgcgcaccac cggctgcaca cgcttgtcga aggtgtagcc gagtgcgcca tacgcgccgt
     7501 ggccttcggt gcgatggcca ttgttccgaa cgccatccca agcatggatg tattcggctt
     7561 gagccaaaac cccatgcgcg tcgtagcgaa gatccgcttc gaggcggtca cgcacggtga
     7621 cgcggtcccc aacggtggtg tagcccatac ccgcgagagt gagcccctcg atcgggtacg
     7681 tctcgaggcg tagcgctacg tccttgtcgt tgtcgttgtc ggtcgggctt tggccgttcc
     7741 cgttgaatac gcccgcgtag tagtagaagt aattgccgag cttcttgtcg atgcgcagac
     7801 cgatgtcgcg ccgatcacca aaggtggtag cgacgcgcga gcgctccggc agcatcagac
     7861 gggccgcgct cgtcactcct tccaagctga tgggggtctt gaactggccg acggagaaat
     7921 ctgcgtagtc cgtcatgaac gtgatgtaga agtcctgcag cggagtatcc gagcggggta
     7981 ccgtggtcgt ggtggtccca accgtggtct tggacgtatc caccaccgtg agcgtcgcgt
     8041 tgcccccgct gaacgccttc gcgccatcga gcatcaacgt gtagccgaag accttgggga
     8101 taatttcacc cttcagtttg atctcggcgc gcctcagtcg cccggtcatg gtgtgcgagg
     8161 cgggggcagt gccgttctca ctatcgtggc tgtagaaggc ccaaaactgg agtagcccgc
     8221 taaactgcaa gaagccgccg tctttgccct gggccaaacg atcggggagc gttggcttcg
     8281 gctcctccac cgcggccggg atcgcggccg gcgcagatgc ggcgggagcc acggcgaacg
     8341 ttgccggagg agccgccaga gcctggtttg gcgcaagctc gggcggcgcg gagggggcgg
     8401 tcgcaggggc gggcggctcg agaccaggag cgaccggcgg cggcgtggga gcgggcgccg
     8461 gtaccgcagg ctcgggaacg ccctgcgcga gaacgggcga agcgacggcg atcagagcga
     8521 gtccgagtaa cgagcgttgc agtgtgtgca tgggcacttc ctttcgaggc gcgacaaaac
     8581 acaccgcgcg tgacgccagc gaaacgccag ggtgacttga cggtaaaccc tgacgaaccg
     8641 gcgtctgttt cgcgaaattc gcgcgacgcg aatcaccctc gagagcgaag ccgcaactgc
     8701 aataggttaa gcgtgggtca cgccacggct tggcgcactt cgagctcggc cttgcgaagc
     8761 gtgaaaaaga accgcgtacc ccgaccgggt tcgctctcga ctttgacgtt gccccccatg
     8821 gcctcgacta aatgcttgac gatggagagc ccaagacccg tgccgcccac gtcgcgggaa
     8881 cgccccgcgt caacccgata gaaccgctcg aagatgcggg gcaggtgacg cgcctcgatg
     8941 ccgggaccgt cgtctcgcac cgaaagctcg aagctctcgc ccacgtcgcg cgcctctacc
     9001 gagacggtcg cccccgccgc gcaatacttc accgcgttgt ccaccaggtt gccgagcacg
     9061 tgttccagcg ctcgtcgatc ggcttgcacc cgagccccgg cggaagaaat cgtattctcc
     9121 aggcgaatct gcttacgctc tgctcgctcg cgaaacaaac tcgagacctg gctgcacacg
     9181 ctgcgtagct ctagcggctc catgttcaaa cgcagcttct gggattcgat gtgcgacagc
     9241 gaaagcacgt cttcgaccag gtcgtgcaat cgcacggcat tacggtcgat gatcccgatg
     9301 aactgccgca gcaccgcggg atcttccggc aagccaacct gcagcgtttc tgcggcggag
     9361 cggattgcgg tgaccggtgt acgcagctcg tgcgagacgt tggcgacgaa gtcgcgccgc
     9421 atcgattcca atttgcgtac ctcggtcacg tcgatgaaca cgccgaagtg ctgcgcaggt
     9481 tcacccgcca cgctcgcaac ccgcacgagc aggcgtcggg gcttgagcgt gcccgcctcg
     9541 agttcacacg aaaccggttc tttggttgcc tcggcgcgat cgaacagctc tttgagttcg
     9601 gtgcgccgga tcacctccaa cagggtgcgt cccaccgcgt ctggaccaat caacaacatc
     9661 tcgcgtaacg ccgggttggt gagcgcgatt cgaccctgcg catccaacaa caacaccccc
     9721 tcctccatgg ccgcgaggat accgcccagg cgatcacgct gttcccgcag gttcaccaac
     9781 acccgagcca gagcatcgaa gcctctttcg atctctcgct cggagtgggt caaaatgcgc
     9841 gcggaagcga cgacttcatc cgagtcccag cgcagcttcg cgaggtgccc cgccgtaacg
     9901 tccagcgcct gccagcggcg cgatctttct cgctcggccc agctagcaaa aaacgtggag
     9961 ctcgccaaaa tccctaccag gcctgccaat cccagtcgtt gacgctcggc ttcaccctgg
    10021 gcggagccat ccagcgcgag ccaaagcggg atccccaaac agacgccatt cacggccagc
    10081 gagagcgcga agcgctgcag gcgcccgcct cgcttcaagt cgcagcctct tcgggggacg
    10141 cgacgaagcg atagccgacc cctcgcacgg tctccacgta gtctcgcgcg gcctcgagct
    10201 tctcccggag ccgcttcacg tgcgtgtcta cggtccgcgt cgtgatgtcc gcctggatac
    10261 cccatacgtc atcgagcaag gcactccgcg tctggacccg atttcgacgc tcatacagcg
    10321 tgactaggag cttgaactcg agcgcggtca gctcgatctc ctgcccttcg acccacacgc
    10381 ggtgcgcgcc gcgatcgatg cgcagccgcc cgaactcgat gtgcgagtct tgcccctcgc
    10441 tctccggctt gccgcgacgc aaaaccgcct gcacccggag cagcagctcg cggacactaa
    10501 acggcttgac gacgtagtcg tcggccccaa tctcgaagcc cacgacgcga tctatctctt
    10561 ctccacgagc cgtaaccatc aagacgggaa tcgacttggt ggtcgcgcct tctttcaggg
    10621 ttttgcagac ttctgtgccc gagatgtcgg gcagcatcag atcca
//
LOCUS       DASTKU010000299         3574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_59233,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000299 DASTKU010000000
VERSION     DASTKU010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59233"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VFQ61_10775"
     CDS             complement(<1..370)
                     /locus_tag="VFQ61_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9954980.1"
                     /translation="MSTSHTGAPASHVSLGQQFRRHAPRYVVGLLLLATYQTAQYWFD
                     TRLRTAINAALTHERDAATWLGVELVATACVALLVRVASRMAIFNAGRYAEYELRQSL
                     SERLLLLGPSFYRRMSTGEIM"
     gene            complement(419..1243)
                     /locus_tag="VFQ61_10780"
     CDS             complement(419..1243)
                     /locus_tag="VFQ61_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9954981.1"
                     /translation="MQNRATLPSPTDDIPNQSDRRARPVTVEDINVVFQPIVDLGAGW
                     TVAYEALVRCKWPGFENPMRLFDWAQNENCIGHLGRVIREVAFGRCDDAPLFVNIHPR
                     ELNEGWLVRPDDPMFFHEHPVYVEVTESAAFDYFDLCLNVLREVSSRGGVYLVVDDLG
                     AGYSNLKRVLDLEPRIVKLDLALVRGIEKNPRQRTLVRQVVALCQELGADVVAEGIET
                     VDELLAVRDTGAHYGQGYLFARPAYPIPRATWPLPRNESRRGRLAGEPNSHAKHRV"
     gene            complement(1339..>3574)
                     /locus_tag="VFQ61_10785"
     CDS             complement(1339..>3574)
                     /locus_tag="VFQ61_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9954982.1"
                     /translation="GFLEAAVDVRVSELVQSSTRNVLLHVHEGKPVRVIGREYPCLGP
                     ERTPADIGSEVDSFLSELPGGTLLEPVDARSVDALFGPKHAGGSRPSPYRLNPWSVYS
                     PDVYEKAAEHLKELFRSEGYLSASVGPVFVQRRRCQKGTMDGQCIPVGPRVRPQLQCS
                     YDPQGLPAEEPAIDPKLSCVEDRSKGVYCEPEVIVNLPIKLGPRSFVQGVEIEGNSSI
                     PKSELLSEANLPDGAPLSQSEVDRARRRLLDRYQEEGFAFAELDSDLEFSPDHRHAKV
                     RFLVSERKRVRVSRIVVRGARTTSENLIRSRIALVSGDLYRRSLVRKTEERLSTLGVF
                     SSVTVTLEDPYVPASEKVVVITVQEKKPQYLDVRPGFSTGEGFRIMFEYGHRNLGGQG
                     VQLTLRSQLGFLPPELIIESDVRRKYEGLGPDELLERRNTATVEFPDIGLGPLFRLRV
                     EGVDVRDNSRDFGLSKDAGILSLFFLPERRLSFQIAGSLERNDATIFGQERECAPVGA
                     FRVPQVPTFVVAQRVGANWDRRDNPLDATSGTFVSASAEYVRARPIGVGTCASGDFIR
                     YTNRVAGYVRLSQRGLALAASFRWGFNQQLRSGSHTYPDRLFFMGGVDSLRGFLQDSL
                     VPEDVARRIMSGQDTIQSIPIRGGDLFVNPRVELRIPLTEDVQTALFVDAGNLWTEPS
                     NIDLSKLRYATGTGLRVGTPIGPLVFDYGFNVDRVLEELLRRARKRPWEDGIGAFHFS
                     IGLF"
BASE COUNT          721 a         1184 c         1068 g          601 t
ORIGIN      
        1 tcatgatctc gcccgtgctc atacgtcgat agaacgaggg gcccagcagc agcaagcgct
       61 ccgagagcga ctgacgcagc tcgtattcgg cgtaacgtcc ggcgttgaag atggccatgc
      121 gcgaagccac ccgcaccagc agcgcaacgc aagcggtagc cactagctcc acgccgagcc
      181 acgtcgccgc gtctcgttcg tgggttagcg cggcgttaat cgccgttcgc agacgcgtat
      241 cgaaccagta ctgggccgtc tgataggtgg cgaggagcag caaacccacg acgtaacgcg
      301 gcgcgtgtcg acgaaactgc tgccccaagc ttacgtgcga agccggggcc ccggtgtggg
      361 acgtgctcat ggtcggctga ctttacggtc cccgcggcga acgtcgagcc cgtcacgctc
      421 aaacgcgatg cttcgcgtga gagttcggct caccagccaa gcgaccgcga cgagattcgt
      481 tccgcggaag tggccacgtc gcgcgcggaa ttggatatgc gggtcgcgca aacaagtagc
      541 cctgcccgta gtgcgcaccg gtatcgcgaa cggccaacag ttcatcgacc gtctcgatcc
      601 cttcggccac cacatccgcg ccgagctctt ggcaaagcgc cacgacctgc cgcacgagtg
      661 tccgctgccg cggattcttc tcgatgccac gcaccaacgc caagtccaac ttcacgatgc
      721 gcggctcgag gtcgagcaca cgcttgaggt tggagtagcc cgcacccaaa tcgtccacga
      781 ccaagtacac gccgccgcgc gacgacacct ctcgcagaac gttcaagcac aagtcgaagt
      841 agtcgaaagc ggctgattcg gtgacctcca cgtaaaccgg atgctcgtgg aagaacatgg
      901 ggtcatcggg gcgaaccaac cagccctcat tcagctcgcg tgggtgaata ttcacgaaca
      961 ggggtgcatc gtcgcagcgc ccgaacgcta cttcacggat cacccgcccg aggtgaccaa
     1021 tgcagttctc gttctgggcc cagtcgaaca agcgcatcgg attctcgaaa ccaggccatt
     1081 tgcagcgaac cagagcttcg taggccaccg tccagcctgc ccccagatcc acgatgggtt
     1141 ggaacaccac attgatgtcc tcgaccgtga ccgggcgagc ccggcgatca ctctggttgg
     1201 gaatgtcgtc cgttgggctg gggagagtgg ctcgattttg catgtacccc aagcgcacaa
     1261 cggcctgccc attcggagag tttaccgctg aacacgaagg tccagagaat cagccgcgac
     1321 gcgaaagccc ctcgccgttc agaacagccc gatgctgaag tggaacgcac cgatgccatc
     1381 ttcccacggg cgtttccggg cgcgccgcag cagctcttcg agcacccgat cgacgttgaa
     1441 accatagtcg aacaccaacg gaccaatcgg cgtacctacg cgcaacccgg taccggtcgc
     1501 gtagcgcagc ttgctcaagt cgatgttgct cggctccgtc cacagattgc ccgcatccac
     1561 gaacaacgcg gtctgcacgt cttcggtcaa aggaatccgc aactctacgc gcggattcac
     1621 gaacaaatca ccgccgcgga tcgggatgct ctggatcgtg tcctgccccg acatgatgcg
     1681 acgagcaacg tcctccggca cgagtgagtc ttgcaggaat ccgcgcaagc tatccacgcc
     1741 gcccatgaag aacaagcggt ccgggtaagt gtgggacccg ctgcgcagct gctggttgaa
     1801 gccccaacga aagctcgcgg ctagcgccaa cccgcgctga gacaagcgca cgtaaccggc
     1861 gactcggttg gtatagcgaa tgaagtcccc cgatgcgcac gtgcccacgc cgatcgggcg
     1921 ggcccgaacg tactccgcgc tcgccgacac gaacgtaccc gacgtcgcgt cgagcggatt
     1981 gtctcgacgg tcccagtttg cgcccacgcg ctgcgcgacc acgaacgtgg gaacctgggg
     2041 aacgcggaaa gcacctacgg gtgcacactc tcgctcttgc ccgaagatgg ttgcgtcatt
     2101 gcgctcgagg gagcctgcga tttgaaacga cagccgccgc tcgggcaaaa agaacagcga
     2161 aagaatgccg gcgtctttgc tcaagccgaa gtcgcgcgag ttgtccctca cgtccacgcc
     2221 ctcgacgcgc agccggaaca acggccccag accaatgtcc gggaattcga cggtcgccgt
     2281 gttgcgccgc tcgagcagct catcggggcc gagcccttcg tacttgcggc gcacgtcact
     2341 ctcgatgatc agctcgggcg gcaagaagcc gagctggctg cgcaacgtga gctgcacccc
     2401 ctgcccgccc aagttgcggt gcccgtactc gaacatgatc cggaagcctt cgcccgtgct
     2461 gaaaccaggg cgcacgtcca agtactgagg ctttttttct tgcaccgtga tgacgacgac
     2521 tttttcgctc gccggaacgt aggggtcctc gagtgtaacg gtgacgctcg agaacacccc
     2581 gagggtgctc aggcgctcct cggttttgcg caccaagctt cggcgataca agtccccgct
     2641 caccaacgcg atccggctac ggatcaagtt ctcgctggtg gtccgcgcac cacgcaccac
     2701 gatgcgagac acgcggacgc gcttgcgttc actgaccagg aaacgcacct tggcgtgccg
     2761 gtggtcgggg gagaattcga ggtccgaatc gagctcagcg aaggcgaaac cttcttcttg
     2821 gtagcgatcg agcaagcgcc ggcgggcgcg atctacctcg gactgagaca acggcgcccc
     2881 gtcgggcaga ttggcttcgg acagcaactc ggacttgggg atactggagt tgccctcgat
     2941 ctccacccct tgcacgaagc tgcgaggccc gagcttgatc ggtagattga cgatcacctc
     3001 gggctcgcaa tagacacctt tgctgcgatc ctcgacacac gagagcttgg ggtcgatggc
     3061 gggctcttcg gcgggtaatc cctgcggatc gtaactgcac tgcagctgag gtctgacgcg
     3121 cggccccacg ggaatgcact gcccatccat cgtgcccttc tgacaacggc ggcgctgcac
     3181 gaacaccggg cccacgctgg ccgacaagta gccctccgat cggaagagct ccttgaggtg
     3241 ctcggccgcc ttctcgtaaa catcggggct gtagacggac cacggattga gtcgataggg
     3301 agatggccgt gatccgcccg cgtgtttcgg cccgaacaag gcgtccaccg agcgcgcatc
     3361 caccggttcg agcagcgtgc cgccgggcag ctcggacaaa aagctgtcga cctcgctccc
     3421 aatgtccgcg ggcgtccgct ccggccccaa acagggatat tcacgcccga tcacgcggac
     3481 cggcttgccc tcgtgcacgt gcaacagcac gttgcgtgtc gaactctgga cgagctcgct
     3541 gacgcgcacg tcgacggccg cttccagaaa gccg
//
LOCUS       DASTKU010000300         5169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1620543, whole genome shotgun sequence.
ACCESSION   DASTKU010000300 DASTKU010000000
VERSION     DASTKU010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620543"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VFQ61_10790"
     CDS             complement(<1..1246)
                     /locus_tag="VFQ61_10790"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9954983.1"
                     /translation="MRDLLGDGTRPADRFVPESEQSGFTNGAESTLLSAVVIDDFELA
                     ATDLAKAATSTSKLAKTVGCDPSATAGQDACAKAFIRSFGARAFRRALDDAQLADYEA
                     LYASSKASAGFAVAIELVLRALLQSPYFLYRVELGSPGALNGAVASLTPEEMAVRLSF
                     LLWGSVPDAPLQQAAREGRLNTAAEVRAQATRMLDDERGRALFGDFHVQWAQLEGLPN
                     QTKPPPFSPEIGRLLIEETEQFVDQVLRKGDGLLSTLLTTPVTYLNQPLAEFYGVKGV
                     TGQSFRPFMFPAGQRAGLLTQGSIMANFAHGTEPSQVLRGKFILAQLACSPPPPPPDN
                     VNATLPAPDPTKSARQQLVELTGTGSCVGCHSMLNPAGFAFEHFDGLGRYRTTERGLA
                     VDPSSSVIVPVDMAGNYGNHEDF"
     gene            1829..3280
                     /locus_tag="VFQ61_10795"
     CDS             1829..3280
                     /locus_tag="VFQ61_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET9954984.1"
                     /translation="MMGQRFCRYGRSGSALLIAVWCITTPACSSDTGTAKGSAKGGGG
                     SSGNSAIASGGRSAGTSGSGESGTTAAGSASGGTSLAGNTSSGARAGSDGTAGTMRPV
                     GGDGGSGATPGVEGGNTSLAGRDGTMTGGNSGAGGNSGAGGNSAAGAPGSSGMSAQGG
                     RALTDPGTEGDGDFEVGPTYSTQPELTDRGARKGKSFQFSMALADSEFFDGKDATLDP
                     SKPVNATRSISVYVPDAYRDGTEAPVLVIQDGPGELNLVRNALDNLTIATDPARKLPP
                     FVAVAVQNGGNDAQGSERGLEYDTLSERYARFIDLEVLPAVEQNPSVKAAYPNLRLTK
                     DARGRAALGCSSGGAAALTMGWFRPDLFSRIITYSGTFVDQQDSDAPEEAQYPLGAWE
                     YHSSESLIANSAKKDLRIFLNVNENDLRSTDAESTHHNWVMANRRTAAALKDKGYHYR
                     FVFGRGRGHCDAGVKSATLADALIWVWRGYPPI"
     gene            3580..4359
                     /locus_tag="VFQ61_10800"
     CDS             3580..4359
                     /locus_tag="VFQ61_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954985.1"
                     /translation="MKNSPHFVGLACVCLAGAAAATGWWRTRAEHSSDPTELRPTPTI
                     REPAVRVDARKPAPRGSVDEPATLAREVEDPALTRLKVALESGDARTRVEGIRQVAAE
                     GVYEALPLLLERGLDRDPDSAVTLIRAIGILTRLAPETDRAGATRRLQTWLAHESVRA
                     ERGADANTVALLKALASIGGADARLTLRNTLDAARLPLPMQTLIVQQLGEQRDPRDAP
                     ALERFLARVETQTAETAMDRELQAEAKAAARDALAGLLARH"
     gene            4441..>5169
                     /locus_tag="VFQ61_10805"
     CDS             4441..>5169
                     /locus_tag="VFQ61_10805"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954986.1"
                     /translation="MSDAGGESEPFGNTEQAVVSGSTCDPDNATGAVSAPARAFLDVI
                     AWAEGTSGHGKDGYNITFGYHTFSSCDRHPNILVCEGICSHAAGRYQFQPATYADYHM
                     PNFWPENQDRAARLLALDHIDLPDRVLTATEFANAVAGLNRVWASFPPSYYNQPTRTL
                     AELREKYCSFISCGTPDAGPPPVRYQVSGGDFNGDGKTDLLTLSPNADGAWNAWAALE
                     LSNSSNTFTSARWNAATPQHMRNGG"
BASE COUNT          984 a         1598 c         1682 g          905 t
ORIGIN      
        1 ggaagtcttc gtgattcccg tagttgccgg ccatgtcgac gggcacgatg acgctcgagc
       61 ttggatccac ggccaggcca cgctccgtgg tgcgatagcg acccaacccg tcgaagtgct
      121 cgaacgcgaa gccggcggga ttgagcatcg agtggcaacc gacgcaggac cccgtaccgg
      181 tcagctcgac caattgttga cgagcgctct tcgtgggatc cggcgccggc aatgtcgcat
      241 tgacgttgtc gggtgggggc ggcggcggcg agcacgcgag ctgcgcgaga atgaacttgc
      301 ctcgcagaac ctgagacggc tccgtaccgt gcgcgaagtt ggccatgatg ctgccctggg
      361 tgagcaagcc ggcacgctgc cctgcaggga acatgaaggg gcggaagctc tgcccggtca
      421 ccccctttac accatagaac tcggcgagcg gttgattcag atacgtcacg ggcgtggtga
      481 gaagcgtgga cagtaagccg tcccccttcc tcaaaacctg atcgacgaac tgctcggtct
      541 cttcgatgag tagccgtcca atctcgggtg agaacggtgg gggcttggtt tgattgggca
      601 gcccctcgag ctgcgcccat tggacgtgaa agtcgccgaa cagtgcgcgg ccgcgctcgt
      661 cgtcgagcat gcgagttgct tgtgcccgca cctccgcggc cgtgttcagc cggccttcac
      721 gcgcggcttg ctgaaggggt gcgtccggga cgcttcccca caataagaac gagagccgca
      781 ccgccatctc ctcgggcgtg agtgaagcga ccgccccgtt gagggcccct ggcgagccaa
      841 gctccactcg gtacaggaag tacggcgatt ggagcagcgc gcgcagcacc agctcgattg
      901 caacggcgaa ccccgccgag gctttcgaac tcgcatagag cgcctcgtag tcagccaact
      961 gggcgtcgtc gagcgcgcgt cggaaggcac gtgcgccgaa gctgcggatg aaggccttgg
     1021 cgcaggcgtc ttgacccgca gttgcgctcg gatcgcagcc gacggtcttc gcgagcttgg
     1081 aggtagacgt cgctgctttt gcgagatccg tagccgcgag ctcgaagtcg tcgatcacga
     1141 cggcgctgag caacgtgctc tcggctccgt tggtgaaacc ggattgttca ctctctggga
     1201 cgaagcgatc cgcggggcga gtgccgtccc ccaagagatc tcgcactaca ttgttgtatt
     1261 cgcgcctcgt gagtcggcgg atgggcgacc caccgacttt gggggctccg caatcgagcg
     1321 aggcgcccgc gaagcccccc gccgttgagt tggggaccgt actcgaagtg ggcggagtag
     1381 aagtcgagct tcccgaacct ccacccccaa ctccgggctc agcgaagccc gaaccgccca
     1441 gtgggcgtgt cgattgcccg ccggaggaag acgatgcgga accagcatga ttcggctcgc
     1501 ctggactctc gcgcgaggaa cacgcggaga cgagcagcac cagcgcgaga gttgcggagg
     1561 agctccgtgg ctggtgcttg tagagtggag gttcggcgcg acgaggccgc ccttctagat
     1621 cgaggcgaag agagcgcact ccaggtactg ccgggggctc gcgcgctttg tgaaacattc
     1681 gtcatcgcgc cgatcacatt gagcgcgttc aaaccgcttg ctcgaaggtc gcgcggcagt
     1741 attttgaacg tactcgacga actaacgtcg tatcacgcca acgcgcagcc gcactttgct
     1801 gattgcgcct gggccgagtg aatggatcat gatgggacaa cgattctgcc gttacggccg
     1861 ttctggttcg gctctgctca tcgcggtttg gtgcatcacc actcccgcct gctcctctga
     1921 tacaggcact gcgaaaggtt ccgccaaagg gggcggtggc agcagcggga actcggcaat
     1981 tgccagcgga gggcgttccg ccggaacgag cggcagcggc gagtctggaa cgacggcggc
     2041 cggttcggcg agcggcggga cgtctcttgc tgggaacaca tcgagcggtg cccgtgcggg
     2101 ttcggacgga actgcgggaa cgatgcgccc ggttggcggc gacggcggca gcggcgcaac
     2161 acccggagtc gagggcggta atactagcct cgccggacga gacggcacga tgacgggagg
     2221 taacagcgga gcgggaggta acagcggagc gggaggtaac agcgcggcag gagcgcccgg
     2281 gagctcgggt atgagcgcgc agggcggtcg cgcgctcacg gacccaggta ccgaaggaga
     2341 cggtgacttc gaggtgggcc caacttacag cacacaaccg gagttgaccg atcgcggcgc
     2401 gcgtaaaggc aagagcttcc aattctcgat ggcgctggct gatagcgagt tcttcgatgg
     2461 caaggacgcg acgctggacc cgagcaagcc cgtcaacgcg acgcgcagca tctcagttta
     2521 cgtcccggat gcttatcgtg acggcacaga agcccccgtg ctcgtgattc aggacggtcc
     2581 cggggaactg aatctcgtgc gaaatgcgtt ggacaatctc accattgcga cggaccccgc
     2641 acgaaagctc cctccgttcg tcgcggtcgc cgtgcaaaat ggcggcaacg acgctcaggg
     2701 cagcgagcgt ggcctcgaat acgatactct ttcggaacgc tacgcgcgtt tcatcgacct
     2761 cgaagtactg cccgccgtcg agcagaatcc cagtgtgaag gccgcctatc cgaacttgcg
     2821 tctcacgaag gatgcccggg gccgcgctgc gctcggctgt agctccggag gcgccgccgc
     2881 actcacgatg ggctggtttc gacccgatct attcagccgg atcatcacct actcggggac
     2941 cttcgtcgat cagcaggaca gcgacgcgcc ggaggaggcg cagtatcccc tcggagcgtg
     3001 ggaataccat tcgagcgaat ccctcattgc aaacagcgcc aagaaggacc tccgaatctt
     3061 cctcaacgtg aatgaaaacg accttcgctc cactgacgcc gagtccacgc accacaactg
     3121 ggtcatggcc aatcggcgca ccgcggccgc tctaaaggac aaggggtatc actaccgctt
     3181 cgtattcggg cgtggccgcg ggcactgcga cgcgggggtc aaatccgcaa cgctcgcgga
     3241 tgccttgatt tgggtctggc gcggctatcc acccatatag gtcaaccagg tgaggtgcgg
     3301 cgcgtccgac gatcgattcc gagtttgaac ccgctcgtgc agttgaatcg gcggttccgt
     3361 cgctcgagtc cgacgtccgc cagtctgctt gctcccgggt tcaggtgatt cggggtacca
     3421 agccatccgg ccgtcccggt cgatggcaat cttggtcgtc tttcgcccgc gaccgaacct
     3481 aaagcagctc cgacgcattc tgtaggtacg agtttctgaa gcgtcaaacg agctgctgtg
     3541 ttcgcagtct gaatgcggcg gctccactgg agatcctgct tgaagaacag ccctcacttc
     3601 gtaggcctgg cctgcgtgtg cctcgcggga gcggccgccg cgaccggatg gtggcggacg
     3661 cgcgcggagc actcctccga tccgaccgag ttgcgtccga cgcctacgat tcgcgaaccc
     3721 gcagttcgtg tcgatgcgcg gaagccagcc ccgcgcggaa gcgttgatga gcccgcaaca
     3781 ctcgctcgag aagtagagga tcctgcgcta acgaggctga aggtcgcgct ggagtcgggc
     3841 gatgcgagaa cccgcgtcga gggaattcga caggtggccg ccgaaggagt ttacgaagcg
     3901 ttaccactcc tactcgaacg aggtctcgac cgagatccgg actccgcggt gacgttgatt
     3961 cgcgcgatag ggatcttgac gcgccttgcc ccggagacgg atcgcgcggg cgcgactcgt
     4021 cgactacaga cttggctggc tcacgaatcg gtgcgagcag agcgtggcgc cgacgcgaat
     4081 actgtcgctc tcttgaaagc gctcgccagc atcggcggcg cggatgcgag actgacccta
     4141 cggaacacgc tggacgcggc gcggcttcct ctgccgatgc agacgctgat tgtacagcaa
     4201 ctgggcgagc agcgagatcc acgagacgcg cccgctctgg aacgtttcct tgcgcgcgtc
     4261 gaaactcaga ctgcggagac tgcaatggat agggaactgc aagccgaggc caaagccgcc
     4321 gcccgtgacg cgctcgccgg cctcctcgct cgccattgaa agaaggaaat accgatgaga
     4381 ataaagggtt gtttgagtcc actaataggg acggtttgga ccgtgttcgc ctgctccagc
     4441 atgagcgatg ctggcggaga atcagagcca ttcggcaata ccgagcaggc ggtagtgtcc
     4501 gggtcgacgt gcgatccgga caacgcgact ggggccgtct cggcgccggc ccgggcgttt
     4561 ctcgatgtga tcgcgtgggc agagggcacc tccggtcacg gcaaagacgg ctacaacatc
     4621 accttcggtt atcacacctt cagttcgtgc gaccggcacc cgaacattct agtttgtgaa
     4681 ggcatctgtt ctcatgcggc gggacgctat caattccagc ccgcgacgta cgccgactac
     4741 cacatgccga atttctggcc ggagaatcag gatcgcgcgg cgcgcctgct cgccctagat
     4801 catatcgact taccggatcg cgtgctcacg gccacggaat ttgcaaatgc agttgcgggg
     4861 ttgaaccgcg tctgggcatc tttcccgcca tcctattaca atcagccgac gagaaccctc
     4921 gcggagctac gcgagaagta ctgcagcttc atctcgtgcg gcacgcccga cgcgggcccg
     4981 ccgccggtgc ggtatcaggt ttcggggggt gacttcaacg gtgatggcaa gacggactta
     5041 ctcacgttga gccccaacgc cgacggcgcc tggaacgcct gggccgcgct cgagctctct
     5101 aactccagca acaccttcac ctctgcgcgt tggaacgccg ccactcctca gcacatgcgc
     5161 aatggcggt
//
LOCUS       DASTKU010000301         7702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1843339, whole genome shotgun sequence.
ACCESSION   DASTKU010000301 DASTKU010000000
VERSION     DASTKU010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7702
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843339"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VFQ61_10810"
     CDS             <1..867
                     /locus_tag="VFQ61_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954987.1"
                     /translation="ALPNAADRLILPGSRRVRTFDFARGRTRVHVLSGCSPNSTLREI
                     ELRGQGRPNVRQVGPFPRLLEQDAQRGWYEEEILEGWCLARLPPWFDRARLLRQALSE
                     LDRFAAPSRVEVAAHAWVDERRAWFAEQLAEEQPRMAKSLPQATLDCLAELASEGPRL
                     RVQLAHGDLQEGNVFALREGGVLILDWEFSHLSFAEYDRLVLGLWMRSGGNILARLRA
                     FVHGEDAHLALAGLPCGYPERRRLVARLLLEELRFFLVESGARDDGYSQGLQAVFQAA
                     QGFVDAVMQERL"
     gene            1011..1478
                     /locus_tag="VFQ61_10815"
     CDS             1011..1478
                     /locus_tag="VFQ61_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954988.1"
                     /translation="MPGTHGRVTGRWAAVTIRPLISSLLWVVACTHHPPSHTAAEYLG
                     GIQDALSATQPRILPTLIITWEADEPDLGQCKRVSGEVVIYLHMGRILQAARSESEVR
                     PLVAEVLSHELAHANMTCTDADHYRMPRPSVVGLEKPDGLKRRFAWETVRPDR"
     gene            1559..2017
                     /locus_tag="VFQ61_10820"
     CDS             1559..2017
                     /locus_tag="VFQ61_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET9954989.1"
                     /translation="MDTPERVLRQSRIIAVVGCSRNPEKSAHSVPKRLRERGYRIIPV
                     NPNVEEVFGERCFSTLGEVATPVDLVLVFRPSEEAPALAQTAIRLGARAFWLQLGLRS
                     EEARRIAGGAGLDYVEDRCAGVEAAQHGIQVSVELDPQSRSPSAPQGSRG"
     gene            2561..4054
                     /locus_tag="VFQ61_10825"
     CDS             2561..4054
                     /locus_tag="VFQ61_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954990.1"
                     /translation="MVKTSTVGVRGLAAIVVTLAAGVWACGESHVALGGGQASVNDAC
                     TRSSECAPSLVCSFSRCHEECTTTADCSNGQRCVGAGNGVRVCQLPAEAQCGGTASCA
                     DGQLCSADNQCREACTSDTACPYGQRCTAAGVCAEPVEVDENGNLKIPGGSPTPVGSS
                     TVPPQGSGGATSTAPSSGTTASAGATNDHPSASGGMNAGGTASSEGGAGGDPDVEEGG
                     AGAGGQTTTMNPVVVHETCDDADRGNATRDKALSIALNTKVQSCFQTDADAEYFEFTV
                     PEAPQQGGYVVVSATDVNFNGAVDMTVESAADDLPIRVWKGLATEDVYGWFAASPGST
                     YRVLLKPNMSTGRISYVFRARFSGVPDEYAPNQTRDTAKRIEVGDSIQAFTFAGHATS
                     EAPMSTAFEDWYMLRLEAGKIEARLDPAADVRGRITLYRSTGEYVTDTFQGNPGVTLT
                     LTKSKLVAGDYYLRVDNFSDPPASSGEGPTPPPHATNPYTFTITQPL"
     gene            4566..5522
                     /locus_tag="VFQ61_10830"
     CDS             4566..5522
                     /locus_tag="VFQ61_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9954991.1"
                     /translation="MTSAAVTELEVPTTLRLSGTLRGNQETDLAANAAGRVLATLVER
                     GNQVSPNQVLARLDVRAAALSAAEARAQAETVTAQEENARRECARYEQLKAKGAVSDF
                     EYERITDQCRTLPLSREAASARARLAAQNVGDGIIRAPFAGVVSERYVEVGQYVRQDT
                     RVVSIASLDPLRLELSVPEAEVGRVKMGADISFRVAAYPDRKYHGTVRYMAGALRSST
                     RDLVVEALVENADKTLLPGMFADVELTTGTQKLPSVPSNALMNRDGQAHAFFVVEGRA
                     EERILSLGPSVADRVSVQRGARVGDQVVVKPSESLANGQRVR"
     gene            5541..>7702
                     /locus_tag="VFQ61_10835"
     CDS             5541..>7702
                     /locus_tag="VFQ61_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9954992.1"
                     /translation="MQWLARLCVRRPVFAGVLMLVVVVLGLVGYTRLGLDQFPNIDLP
                     FVLVTTRLDGAAPEEVETDISDKIEGAVNTIEGVDELRSISSEGFSQVSVVFKLDKNG
                     DVGAQDVRDKVNGILKDLPKGIDPPVISKVDPQAAPVLLVALKSKRDIRDLTELADKR
                     VRRQIESTSGVGQVTIVGGRARQIHIYLNPVRLRSYNLSAVDVQRAVASQNLSTPGGS
                     LDTGPESITLRVQGRVEDVGSLERIVVRERDGHAIRISDVGRVIDGAEDVASVASYDG
                     ERTVVMSVRKQSGTNTVQVVDQVKKRLEEVRRGLPKGVDLEIVRDNSQSIRTGVDSVK
                     EHLAVGAVLAAFVVLLFLGNARSTIIAAIAIPISIIGTFALMWLEGFSLNMLTLLALA
                     LAVGIVIDDAIVVLENITRHVEEKGTKPFPAAVIATKEIGLAVLATTLSLMAVFVPVA
                     FVGGIPGRFLKNFGYTMAFSVGVSLFVSFSLTPTLCARFLRPHGHAPSRMAGLVDLFY
                     RPIERVYMRLLGWSMRNRWAIVVASALSLGSCVPLMKRVPTSFIPIDDQAQFEVSLRT
                     PEGTSAEETALVAERVAQEVRRLPGARHTVVTVAGGDQQTENLASIYVALADPKEREL
                     SQYQLMDRARKEVLAKLPKELRVSVAEVAAFSTGASSAAVQYVLAGPDLRKIEEYVGR
                     MGPDIRKSPAVVDFDTNLIVGKPQVKVEIDRDRASDLGV"
BASE COUNT         1361 a         2261 c         2639 g         1441 t
ORIGIN      
        1 gctctgccga acgccgcaga tcggctcatt ttgcctggat cacgtcgggt gcggacgttc
       61 gactttgcgc gcggacgaac tcgggttcac gtgctctcgg gttgctcacc gaacagcacg
      121 ctgcgcgaga tcgagctgcg cgggcaaggc cgcccgaacg tgaggcaggt ggggcctttc
      181 ccccgattgc tcgaacagga cgcccagcgc ggttggtacg aagaggaaat cctggagggc
      241 tggtgtttgg cacggctacc gccgtggttc gatcgcgctc gcttgctccg gcaggcgctg
      301 agcgagttgg accgctttgc ggccccatct cgagtcgaag tggccgcgca cgcctgggtc
      361 gatgaacgaa gggcgtggtt tgcagagcag cttgcagaag aacagccgcg catggcgaag
      421 tcgctgcccc aagccaccct cgattgcctc gcggagctgg cgagcgaagg accccgcctt
      481 cgagtgcagc tcgcccacgg ggacctacag gagggcaatg tattcgcgct ccgcgagggc
      541 ggagttctga tcttggattg ggagttcagt catctctcgt tcgccgaata cgatcggctc
      601 gtgctcggcc tctggatgcg ctcggggggc aacatcttgg cgcggctcag agccttcgtt
      661 catggcgaag acgctcacct ggcgctcgcc gggttacctt gcggctaccc ggagcggcgc
      721 cggctcgtgg cccgcctgtt gctcgaggag ttgcgcttct ttctcgtcga atcgggagct
      781 cgtgacgatg gctattcgca ggggctccaa gccgtctttc aggcggcaca aggcttcgtg
      841 gacgccgtga tgcaagagcg cttgtgagcg agtacggatt tcggcaggcg ttgcacccgt
      901 tttgaggact cgttcatcgc tggtgggatg cggggcccac tcactctcac gtgctccact
      961 ctttctcgtg ggctgcggcg tttcgcaggg tgctgccggc gtggtgcaga gtgccgggaa
     1021 ctcacggccg agtgacgggg cgatgggcgg ccgtaacgat ccgcccgctt atctccagcc
     1081 tgctttgggt ggtggcctgc acgcaccacc cgccgagcca taccgctgcc gagtatctcg
     1141 gaggcattca ggacgcgctt tccgcgaccc agccacgcat tctgcccacc ctgatcatca
     1201 cctgggaggc cgacgaaccc gacctaggtc agtgcaaacg ggtatccggc gaggtcgtga
     1261 tctacctcca catgggccgg attctgcaag ctgctcgctc cgagtcggag gtgcgcccgt
     1321 tggtggcgga ggtgctgagc cacgaattgg cccacgccaa catgacgtgc acggacgcgg
     1381 atcactaccg aatgccgcgc ccgagcgtgg tgggtctcga gaaaccggat gggctgaagc
     1441 ggagattcgc ttgggaaacg gtaagaccgg ataggtaaga gcatttcctt aattgccagc
     1501 agtcttcggc tgaatcttgg ccgagtagct gggagcggtg tagccatgct cggaagccat
     1561 ggatactccg gagcgcgtcc tgagacagtc acggatcatt gccgtggtgg gctgcagccg
     1621 taacccggag aaatcagccc acagtgtccc caagcgcttg agggaacggg gatatcgaat
     1681 cattcccgta aaccccaacg tcgaagaggt gttcggcgaa cgctgcttct cgaccttagg
     1741 cgaagtggcc actccagttg atttggtgct cgtgttcagg ccgtcggagg aggctcctgc
     1801 gctggcccag acggcgatcc ggctaggtgc acgcgccttt tggctgcaac ttggcctgcg
     1861 ctcggaggaa gcgcgtcgta tcgccggagg ggcggggctc gattatgtcg aggatcgctg
     1921 cgccggcgta gaagctgccc agcacggcat tcaggtttcg gttgagctcg acccgcaatc
     1981 ccgtagtccg tcggcaccgc aggggtctcg tggatagcac cgccgtttgg ctatgagggc
     2041 tccttccagc atgggattgc ctgtggatgt gttgaggatt cttgtcgatc tgtccctggc
     2101 ttttgggggc cacacacagc gatttctgac tctgaggagt gctgttcgga aagcgcttaa
     2161 gcgatcttcg tagaggtgaa tgtgtgtgtg ctcgacgcga agcaaggcga gaaatcgagc
     2221 aagaatttag gcacgagcgt gatcgcacgc ggcttcgaga ggcacctagg atgtccgttc
     2281 ggcgttgggg atatcccgag tgctgctgcg gaaaaacggc attggtgctt gtccccagcg
     2341 ccgtcgcact cgcgaacggg agctgcacgt aacgcacgat ccagccaaat tcagcggtga
     2401 tcgcgctgcg cgtgtgctgt tcgttggtgg cacactcatg cacacaccga gcgtgcgccg
     2461 ctcacaattc agcgagaaaa gctgcgggat gacactgtca aagtattgct ttgataccag
     2521 ccctgaagta cgttccgggt tgagaagagg gagaacttgc atggtgaaaa ccagcacagt
     2581 tggcgtccgc ggtttggcag ctatcgttgt caccctcgcg gctggagttt gggcctgcgg
     2641 cgaaagccac gtcgcattgg ggggcggtca agcttctgtg aatgacgcct gcacgcgcag
     2701 ctcggagtgc gcgccctcct tggtttgctc gttcagtcgc tgccacgaag agtgcacgac
     2761 cacggctgat tgcagcaacg gccagcgctg cgtcggggcc ggcaatgggg ttcgcgtttg
     2821 ccagcttccg gccgaagcgc agtgcggggg gaccgccagt tgtgctgacg gccagttgtg
     2881 ctccgctgac aaccagtgcc gcgaagcttg cacctcggat acggcctgcc cctacggcca
     2941 acgctgcacg gctgccggcg tatgcgcgga gcccgtggaa gtcgacgaga acggcaacct
     3001 gaagattccc gggggaagcc ccacgccggt tggatcgagc acggttccac ctcaaggatc
     3061 tgggggcgcc acctcgacag caccttcatc cggtacgacg gcgtccgccg gtgccaccaa
     3121 cgatcatcct tctgccagcg gcggcatgaa cgcaggaggg accgcttcca gcgaaggcgg
     3181 cgcgggcggt gatcccgacg tcgaagaagg tggcgcgggc gcgggcggtc agaccaccac
     3241 gatgaacccg gtcgtggtgc acgagacctg cgacgacgca gatcggggca acgccacgcg
     3301 ggacaaggcg ctgagcatcg cactgaacac gaaagttcag agttgcttcc aaacggacgc
     3361 ggacgcggag tacttcgagt tcacggttcc cgaggcgccc cagcaaggtg ggtacgtggt
     3421 cgtgagcgcc acggacgtga acttcaacgg cgccgtcgat atgactgtcg agagcgcggc
     3481 tgacgatctg ccgattcgag tctggaaagg gttggcgacg gaagacgttt atggctggtt
     3541 tgcggcttcg ccgggcagca cgtaccgcgt gcttttgaag cccaatatgt cgacgggtcg
     3601 gatctcgtac gtgttcaggg ctcgcttcag cggagtgccg gacgagtacg cgccgaacca
     3661 aacgcgcgat acggcgaagc gcatcgaggt gggagactca atccaagctt tcacgttcgc
     3721 aggtcacgcg acgtccgaag cgccgatgtc cacggcattc gaagactggt acatgctgcg
     3781 tctggaggcc gggaagatcg aggctcgttt ggacccagcc gcagacgtgc gcggccgtat
     3841 cacgctctac cggtccacgg gcgagtacgt gactgacacg ttccagggca accccggcgt
     3901 gacgctgacg ctgaccaaga gcaagctcgt cgcgggtgac tactacctcc gcgtcgacaa
     3961 cttctctgac ccgccggcgt cttcggggga ggggcccact ccgccacccc acgcgactaa
     4021 cccgtatacc ttcacgatca cccaacctct gtaagtcgcc tagtccgcat gcccaccctg
     4081 gccggatcga aacagggctt tacgggcggg ctatctgcaa gtcgtcaatc gttggtacgc
     4141 cgggcacggc ggttggccgg gcggttggtg gcccggtgcg ggctcagctc aacacaacac
     4201 ggctttcgaa ggctccacgg caagtggggg tgggccgctt caagaatgtt gaaggtagtc
     4261 accgtgcccg agcatgctcg atgggcgatc tggttcgagc tggatcactc gcagtcttcg
     4321 cggttgagca gcggaagtga ttgtcgcccg cggtgagggt gcggccgtca ctgtggggga
     4381 cgcgggatcg aaaaaaacac gagccattcg gaggctcggg gtccataaat ggcggcgcca
     4441 tgcttcacgc tgcccggtcc gctgctcttc ctcttctcct atctctggct tgtgctcttc
     4501 cgtccggctg caagcgtaat gttcaggaag tgagcgacgc ggggcgtact gaaactccag
     4561 cggaggtgac ttccgcggcc gtgaccgagt tggaggtgcc tacgacgctc cgcctctccg
     4621 gtacgctgcg cggcaatcaa gagacggatc tcgccgctaa cgcggctggg cgggtgctcg
     4681 cgacgctcgt ggagcgcggc aaccaagtgt cgcccaatca agtcctggcg cggctggacg
     4741 tgcgggccgc ggccttgtct gcagcagagg cgcgtgccca agcggagacg gtcaccgctc
     4801 aagaagagaa cgcccggcgc gagtgtgccc gctatgaaca gctcaaagcc aagggcgcgg
     4861 tcagcgattt cgaatacgag cgcattactg accaatgccg cacattgccg ctgtctcgcg
     4921 aggctgcgtc ggcgagggcg cgcttggccg ctcagaacgt gggcgatggg atcattcgcg
     4981 ctccttttgc aggggtggtg agcgagcgtt acgtggaggt ggggcagtac gtgcggcagg
     5041 acactcgagt ggtctcgatt gcgtctttgg acccactgcg cctcgagctt tccgtgcccg
     5101 aggcggaagt gggcagggtc aagatgggcg cggacatttc gttccgcgtc gccgcgtatc
     5161 ccgaccgcaa gtatcacggc accgtgcgct acatggccgg cgcgctacgc tccagcaccc
     5221 gcgatttggt ggtcgaagct ttggtcgaaa atgcggacaa gacgctgctc ccgggcatgt
     5281 tcgccgatgt ggagctcacg acgggaacgc agaagctacc cagtgtgccg agcaacgctc
     5341 tcatgaacag ggacgggcaa gcgcacgcct tcttcgtggt cgaaggacga gccgaggagc
     5401 gcattctgtc gctgggaccg agcgtcgcag atcgcgtgag tgtgcaacgc ggagcgcgag
     5461 ttggggatca ggtcgtggtg aaaccgagtg agtctctagc taacggccag cgcgtgcgct
     5521 gatcgggaag gcaaaatccc atgcaatggt tggctcgttt gtgcgttcgc cgcccggtat
     5581 ttgcgggcgt gttgatgctg gtcgtggtcg tgctcgggct ggtgggttac acccggctcg
     5641 ggctggacca gttcccgaac atcgacttgc cgttcgtgct ggtgacgacg cgcctcgatg
     5701 gcgcggctcc cgaagaagta gaaaccgaca tcagtgacaa gatcgaaggc gcggtcaaca
     5761 ccatcgaagg tgttgacgag ctgcgttcga tctcgagcga gggcttttct caggtttcgg
     5821 tggtcttcaa gctcgacaag aacggggacg tgggtgccca ggacgttcgc gacaaagtga
     5881 acggcatcct gaaagacctg ccgaaaggca tcgatccgcc cgtcattagt aaggtcgacc
     5941 cgcaggcggc gcccgtgttg ctcgtagcgc tgaagtcgaa gcgggatatc cgcgacctca
     6001 ccgagcttgc ggacaaacgg gtgcgccgac aaatcgagag cacctcgggc gtaggccagg
     6061 tcacgatcgt gggcggtcgc gcccggcaga ttcacattta cctgaacccg gtgcggctcc
     6121 gctcgtacaa cttgagcgcg gtcgacgtgc agcgcgcggt cgcttcgcaa aacttgtcga
     6181 caccgggcgg cagcctggat accggccccg agtcgatcac gctgcgcgtg caaggtcggg
     6241 tcgaggacgt gggcagcctg gagcgcatcg tggtgcgcga gcgtgacggg cacgccatcc
     6301 gcatttcgga cgtggggcgg gtgatcgacg gggccgagga cgtcgcgagc gtcgcgagtt
     6361 acgacggcga gcgcacggtg gtgatgtcgg tccgcaagca atccggcacc aacaccgtgc
     6421 aggtcgtgga tcaagtgaag aagcgcctcg aggaggtacg acgaggcctg ccgaagggcg
     6481 tcgacctcga aatcgtgcgc gacaactcgc agtcgattcg gaccggcgtg gactcggtga
     6541 aggagcactt ggccgtcggc gcggtgctcg ccgcgttcgt ggtgctgctg ttcttgggca
     6601 acgcgcgcag cacgatcatc gcggccatcg cgattccgat ctcgatcatc ggcacgttcg
     6661 cgctgatgtg gctcgagggc ttcagcttga acatgctcac gctgctagcc ctggcgctcg
     6721 cggtgggtat cgtgatcgac gacgcgatcg tggtgctgga gaacatcacc cgccacgtgg
     6781 aagagaaggg gacgaagccg tttccggcgg cggtgattgc gaccaaggag atagggctgg
     6841 ccgtgctcgc gaccacgctg tcgctgatgg ccgtgttcgt gccggtggcg ttcgtcggcg
     6901 gaatccccgg gcgcttcctg aagaacttcg gctacaccat ggccttctcg gtcggggtgt
     6961 cgctgttcgt gagcttctcg ctgacgccga cgctgtgcgc gcgcttcttg cggccgcacg
     7021 ggcacgcgcc gagccgcatg gctgggttgg tggatctatt ctatcgcccg atcgagcgcg
     7081 tctatatgcg cctgctcggg tggtcgatgc gcaatcgctg ggcgatcgtg gtggcgagcg
     7141 cgctttcgct cgggtcgtgt gtcccgttga tgaagcgggt tcccacgagc ttcatcccga
     7201 tcgacgatca ggcgcagttc gaggtgagcc tgcgtacgcc ggagggcacc agcgccgaag
     7261 agacggcctt agtggccgag cgcgtagccc aggaggtgcg tcgcttgccg ggggcccggc
     7321 atacggtggt gaccgtcgcc ggaggtgacc agcaaacgga gaacctcgcg tcgatctatg
     7381 ttgcgctggc cgatccgaag gagcgcgagc tctcgcaata tcagctgatg gatcgagcgc
     7441 gtaaagaggt gctcgcgaag ctgcccaagg agttgagggt ctcggtcgct gaggtggcag
     7501 cgttctcgac gggggcttcg tccgcggcgg tgcagtacgt gctcgccggg ccagacctgc
     7561 gcaaaatcga agagtacgtg ggacgcatgg ggcccgacat tcgcaagagc ccggcggtcg
     7621 tcgatttcga taccaacctg atcgtgggca agcctcaggt gaaggtcgag atcgatcgag
     7681 atcgtgcttc ggatctgggc gt
//
LOCUS       DASTKU010000302         5153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1621032, whole genome shotgun sequence.
ACCESSION   DASTKU010000302 DASTKU010000000
VERSION     DASTKU010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1621032"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..773)
                     /locus_tag="VFQ61_10840"
     CDS             complement(78..773)
                     /locus_tag="VFQ61_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954993.1"
                     /translation="MAQIRSVRCVAQVLFALGAVACGGGDKPANSQNLQQQQCPPGQY
                     FDGQLCQTPGAATGQPQPQGVPATGTPTAGVPTAGTPTVATPPLATSAPGTPAQQLDP
                     TAAAAATQLLGALAQSSAPGAKPVGTAIAGNFQQGQTLETQVQMNPGKCYTVVGAALP
                     TVTNLDIELVPVMPIPGVSPVLAADQTQGSTAVVAPAPNCYKWALPVGAPVKVVLRVS
                     AGQGMAAAQVYEK"
     gene            complement(1041..2225)
                     /gene="moeB"
                     /locus_tag="VFQ61_10845"
     CDS             complement(1041..2225)
                     /gene="moeB"
                     /locus_tag="VFQ61_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HET9954994.1"
                     /translation="MSKTYSDLFADVRKNVRTISLDELKQRLEKQAERPFTLVDVREK
                     EEYRAGFIPSAVHISRSFLEMQAEQKLPDKSAEIVLYCAGGTRSAFAARTLADLGYTN
                     VLSANPGFVRWKDLKYPIESPANLTPAQLDRYSRHILLPEVGERGQEKLLQARVLLLG
                     AGGLGAPASLYLAAAGVGTLGLVDADTVDASNLQRQIVHATSRLGMPKVDSAEVALKD
                     LNPDVKIVKYQERVNSGNVDRIFGDKWDVIIDGCDNFPTRYLVNDASLFHRIPVVHGS
                     IFRFDGQVTTFKPFVGPCYRCLYPEPPPPHLAPSCAEAGVLGILPGIIGVLQATEAIK
                     LILGQGDPLVGRLLTYDSLKMSFRVLKLRRDKNCPTCGENPSITSYIDYEGFCAGGPA
                     HS"
     gene            2680..4383
                     /gene="pilB"
                     /locus_tag="VFQ61_10850"
     CDS             2680..4383
                     /gene="pilB"
                     /locus_tag="VFQ61_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HET9954995.1"
                     /translation="MSQNRLGELLVREKLISLQQLRKAQDEQKRTGQNLGYTLARLGY
                     VQDGEITNFLSAQYRLPAINLDEYEIDQDVLKLVGREVCEKHRIIPVSRSGSSLIVAM
                     SDPTNLNAIDDIKFLTGYNVEPVVASETAIQAGIERYYNAGPSYEEVMADFDLGDEDI
                     DFSADEEEISAVELERASADAPVVRLVNVLLLNAIRKGASDIHCEPYEKRFRVRYRID
                     GVLHEEMAPPLKLKNALVSRLKIMSQLDIAERRLPQDGRIKLKLGKGREMDFRVSVLP
                     TMWGEKVVLRLLDKGNLQLDMTKLGFDVAPLKDFQWAINQPWGMVLVTGPTGSGKTTT
                     LYSALSDLNKPGVNISTAEDPVEYNLPGINQVQMHDDIGLNFAMSLRSFLRQDPDVIM
                     VGEIRDFETAEIGVKAALTGHLVLSTLHTNDAPATISRLLNMGVEPFLITASVNLVLA
                     QRLARRICNDCRRESDVEPQTLIDLGCSQEQIATGKIYRGAGCATCNSTGYKGRVALY
                     EVMRFHDDLKEMVLQGASAAELKAAAIKRGMITLRMAGIKKMLEGITTPEEILRCTMA
                     D"
     gene            4404..>5153
                     /locus_tag="VFQ61_10855"
     CDS             4404..>5153
                     /locus_tag="VFQ61_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET9954996.1"
                     /translation="MSGVEEGQKVNLHQLLRAMIEKGASDMHITTGAPPLLRIDGAVV
                     PLKLPPLSPIETKQLCYSVLTEEQKIQFEKNKELDLSSGVKSLSRFRANIFMQRGAVS
                     GAFRAIPFKILSFDELGLPPAVAELTTKPRGLVLVTGPTGSGKSTTLASIIDKINSET
                     RQHIMTVEDPIEYLHPHKRSIVNQREVGSDTSSFKYALKYVLRQDPDVVLVGEMRDLE
                     TIEAALTIAETGHLVFATLHTNSAIQSINRII"
BASE COUNT          986 a         1714 c         1550 g          903 t
ORIGIN      
        1 agctctgttt gcccgagcga gcggcaaacg ccgctcgctc ggccctggtg ggcccattca
       61 gcccaactca gtgcaattca cttctcgtaa acttgagcgg cggccatgcc ttgcccagcg
      121 ctcacgcgca gcaccacttt cacgggcgcc ccgaccggca gtgcccactt gtagcagttg
      181 ggtgcgggcg ccacgacggc cgtcgagccc tgagtttgat cggccgcgag caccggtgac
      241 acccccggga tcggcatgac aggcacgagc tcgatatcga gattcgtaac cgtcggcagc
      301 gccgcaccca ccaccgtgta gcacttgccc gggttcatct gcacctgggt ctcgagcgtc
      361 tgaccttgct gaaagttgcc ggcaatcgcg gtgcccacgg gcttcgcgcc gggagcgctg
      421 gattgcgcca aagcaccgag cagctgcgtc gcggcggcgg ccgccgtcgg atcgagctgc
      481 tgcgcaggcg tacccggagc agacgtcgcg agcggagggg tcgcgaccgt cggcgtccct
      541 gccgtaggca cccctgcggt gggcgttccc gtagccggca ccccttgcgg ctggggctgt
      601 cccgttgcgg cgcccggggt ttggcacagc tgcccgtcga agtactgacc ggggggacat
      661 tgctgctgtt gaagattctg gctgtttgcg ggcttgtcac cgccaccaca ggcgacggcg
      721 cccagcgcga atagaacctg ggcgacgcag cgaacggacc gaatctgagc catctcttga
      781 gctactccat ttcgcgacgc cgagccgggg tcgcgcgcgg cagtctataa cagagtcgct
      841 ccggaaatca ccgggcctcg gctcgcgcgc ggttcccctg caaacccgca gaaaatcggg
      901 ctccgacgaa cgaagcccga ttttcagctc aacgtttctc gccgacgagg gcgattgttg
      961 gcgaaaggaa cccgctcagg atggccgcgg gactcgatgt gctaccgagt gtgcaagcgc
     1021 cttcgcgctc atcgaaaagc ctaactatgt gcgggaccgc cggcgcagaa cccctcgtaa
     1081 tcgatgtagc tcgtaatgct ggggttttca ccgcacgtcg ggcagttctt gtcccgacgc
     1141 agcttcaaaa ctcggaagct catcttgagt gagtcgtacg tcagcaagcg gcccaccaga
     1201 ggatctccct gaccgaggat tagcttgatt gcctcggtcg cctgcaacac accgatgatt
     1261 ccgggcagaa tcccgagcac gccggcttcg gcgcaggaag gagccaggtg cggcggaggc
     1321 ggctcaggat acaagcagcg gtagcacggc cccacgaacg gcttgaacgt cgtgacctgc
     1381 ccatcgaagc ggaagatcga gccatgcacc accggaatgc ggtggaacaa cgaggcgtcg
     1441 ttgaccaggt agcgcgtcgg gaagttgtcg cagccgtcga tgatcacgtc ccatttgtcc
     1501 ccgaagatcc ggtcgacatt gccgctgttc acgcgctcct gatacttcac gatcttcacg
     1561 tcggggttca gatccttgag cgcgacctcc gcgctatcca ccttcggcat cccgagccgc
     1621 gacgtagcgt gcacgatttg acgctgcaaa ttcgaggcgt ccaccgtgtc cgcgtccact
     1681 agcccgagcg tgcccacgcc cgcggccgcg aggtagagcg acgccggcgc gccgagcccg
     1741 cccgcgccca gcagcaaaac ccgcgcttgc agcagcttct cctgcccacg ctctccgacc
     1801 tccggcaaca agatatggcg agagtagcga tcgagctgcg caggtgtgag attggcaggc
     1861 gactcgatcg ggtacttcag atctttccaa cgcacgaagc ccgggttggc gctgagcacg
     1921 ttcgtataac ccaggtccgc cagcgttctc gccgcgaacg cgctgcgcgt gccgcccgca
     1981 caatacagca cgatttcggc ggacttatcg ggcagctttt gctcggcctg catctccagg
     2041 aagctgcgag aaatatgcac ggcagacggg atgaatccgg cccggtactc ctccttctcg
     2101 cgcacgtcga ccagcgtgaa aggacgctcc gcctgcttct ccaggcgctg cttgagttcg
     2161 tcgagcgaga tcgtgcgaac gttttttctg acgtcggcga atagatccga gtaggtcttg
     2221 gacatccggc ttccttaaag tcgcattttc cgatgtggaa attcgggatt gggagttttc
     2281 ggcctttctg ccccctcgtc aaccccctag ctcgggcagt gaggcacgtt tctacgcgcg
     2341 gacgcaggac gagcccgcga tcgagccgca tacgccctgt tttcagtata gttacgcagc
     2401 gcgcgctcat gcctctcgct ttccagcttc gctccatctc cgccggcttc gagccctgga
     2461 tgccgcccgt cacgggcctg ctctcccgtc cggacgagag cttcccgttc cacgtcagga
     2521 gcacgcgggg attaacttgt ctcgtgactc tgtcgcattc tgaggaaact cccgccagcc
     2581 caccacttca tcccgctccc cgcgcacgcg cccactccgg tctcgaggtc gccttgaggc
     2641 ggtacggcct ggtcactctc tgcgcaagga atcgcactca tgtcgcaaaa ccgtctcggt
     2701 gagctgctcg ttcgagagaa gctcattagc ctgcagcagc tccgcaaggc tcaagacgaa
     2761 cagaagcgga ccgggcagaa cctcggctac acgctggccc gcctgggtta cgtccaagac
     2821 ggggagatca cgaacttcct gtcggcccag tatcgcctgc ccgcgatcaa cctggacgaa
     2881 tacgagatcg accaggacgt gctgaagttg gtgggccgcg aggtctgcga gaaacaccgc
     2941 atcatccccg tttcgcgctc gggttcatcg ctgatcgtgg cgatgagcga tccgaccaac
     3001 ctgaacgcga tcgacgacat caagtttctg accggctaca acgtcgagcc ggtcgtcgcg
     3061 agcgagaccg ccatccaggc gggcatcgag cgctactaca acgcgggccc ctcctacgag
     3121 gaggtgatgg ccgacttcga cctcggcgac gaagatatcg actttagcgc cgacgaagaa
     3181 gaaatcagcg cggtggagct cgagcgcgcc agcgccgacg cgcccgtggt gcgcttggtc
     3241 aacgtgctgc tcttgaacgc gatccgcaaa ggcgcgagcg atatccactg cgagccttat
     3301 gagaagcgct tccgcgtgcg ctaccgcatc gacggcgtgc tgcacgagga gatggcgccg
     3361 ccgctcaagc tcaagaacgc gctcgtgagc cgcctcaaga tcatgagtca gctcgacatc
     3421 gccgagcgtc gcttgccgca agacgggcgc atcaagctca agctcggcaa aggtcgcgag
     3481 atggatttcc gcgtctcggt cctgcccacg atgtggggag agaaggtcgt gctgcgcttg
     3541 ctcgacaagg gcaacctgca actcgacatg accaagctcg gcttcgacgt cgcccccctg
     3601 aaggactttc agtgggcgat caaccagccc tggggcatgg tgctggtgac aggaccgacc
     3661 ggctccggca agaccacgac gctctactcc gccctgagcg acctcaacaa acccggcgtc
     3721 aacatcagca ccgccgaaga cccggtcgag tacaacctgc ccggcatcaa ccaagtgcaa
     3781 atgcacgacg atatcgggct caatttcgcg atgagcctgc gctcgttcct gcgccaagat
     3841 ccggacgtga tcatggtggg cgagatccgc gacttcgaga cggccgagat cggcgtcaag
     3901 gccgcgctca ccgggcactt ggtgctctcg acgctgcaca ccaacgacgc gcctgccacc
     3961 atctcccggc tgctcaacat gggcgtcgag ccgttcttga tcacggccag cgtcaacctc
     4021 gtgttggcgc agcgcctcgc ccgccgtatc tgcaacgact gccgccgtga gtcggacgtg
     4081 gagccccaga cgctaatcga cctcggctgc tcacaggagc aaatcgcgac cggtaagatt
     4141 tatcgcggcg ccggctgcgc cacctgcaac agcaccgggt acaagggccg cgtcgcgctc
     4201 tacgaagtga tgcgcttcca cgacgacctg aaggagatgg tgctccaggg tgcgtccgcg
     4261 gcggagctca aggcggccgc catcaaacgc ggcatgatca cgctgcgcat ggctgggatc
     4321 aaaaagatgc tcgagggcat caccacgcca gaagaaatcc tgcgttgcac gatggccgat
     4381 tgaattccta cgcgagaggt tccatgagcg gcgtcgaaga gggccagaaa gtcaacttgc
     4441 atcaactgct ccgcgccatg atcgagaagg gcgcgagcga catgcacatc accaccggcg
     4501 cgccgccttt gctgcgcatc gacggtgcgg tggtgccact gaagctcccg ccgctctccc
     4561 cgatcgaaac caagcagctc tgctattcgg tgctgaccga ggagcagaag attcagttcg
     4621 agaagaacaa ggagctcgat ctctcctccg gagtgaagag cctgtcgcgc ttccgcgcca
     4681 acatcttcat gcagcgcggc gcggtctcgg gagccttccg cgccatccca ttcaagatcc
     4741 tctccttcga cgagctcggg ctgccgccgg ccgttgccga gctcaccacc aagccgcgcg
     4801 ggctcgtgtt ggtgacgggc ccaaccggct ccggcaagag caccacgctg gcctcgatca
     4861 tcgacaagat caactccgag acgcgtcagc acatcatgac ggtcgaggac cccatcgagt
     4921 acctgcatcc gcacaagcgc tcgatcgtca accagcgcga agtcggcagc gacacttcga
     4981 gcttcaagta cgctctgaag tacgtactgc ggcaggatcc ggacgtggtc ttggtgggtg
     5041 agatgcgtga cctcgagacg atcgaggccg cgttgaccat cgcggagacg ggtcacctcg
     5101 tcttcgcgac gctccacacc aactccgcca ttcagagcat caaccgcatc atc
//
LOCUS       DASTKU010000303         6222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1174642, whole genome shotgun sequence.
ACCESSION   DASTKU010000303 DASTKU010000000
VERSION     DASTKU010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174642"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VFQ61_10860"
     CDS             complement(<1..809)
                     /locus_tag="VFQ61_10860"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9954997.1"
                     /translation="MRHSLCTDAGERSASSHLEFDTTFVRSVPAQTGEESLFAGPESA
                     ARRAHRDRVGLGGLALLALIGLTVGGFRQSSSVTADPTAGLPRNSFHAAESSAPKLDA
                     TQRIQACIAGEGTVCNALGVEIIQHEGFSGPNLQAARVSFDRACNAGNPIGCNNLGVV
                     YENTRSEFGEDLDAKAAKLFRKACDGSSGDGCSNLGVMYENGWGIPQSLTRAREMYLR
                     ACDSGNALGCSNLGALYAEGRGVPKDLPRAQGLFDRACSGNSEVGCHNLAVV"
     gene            complement(982..3201)
                     /locus_tag="VFQ61_10865"
     CDS             complement(982..3201)
                     /locus_tag="VFQ61_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9954998.1"
                     /translation="MKRSVRLAGLRASKVFASRFFASGTTVQFARSNAATQPDFAKPD
                     SARVARASWWLLTAPVLVASPLMGCSGSIDDTGNLPGSGGFPVNPMGGAAGIGGALSG
                     GDRGGFAGAAPQGGAIGSGGSAAQGGAAAGTLTTGMGGSSTATAGAAGAGACQAGQAS
                     CSGSCVDLQSNAQNCGQCGRPCSADQVCSAGQCTCAAPRAVCSGKCVDLQTDAANCGT
                     CGTPCGAGLACAAGLCQCAAGQLRCGAACVDPMKDLQNCGGCGTPCALGQSCTAGQCV
                     ESNGGAAGPDGCSGLAQNVTLSQVDVFQSVRVRVMNAGQAVAAAARNTDVVAGREAMF
                     RLFATVGSGWVARNISGRVLVENNGQVDVFYTKKSLAGSSQEGTLDSTIQVKVPKEKI
                     TTATRYQVELVECGGAPGGAVQSPKFPATSSTPLEARTTGVVKVRVIPLTSGSNKPDT
                     SEAALQIYRDEVMAIYPVTDVQITVGDSLSVADPKDWTTMLDQVRAKRRQDAPAADIY
                     YYGLLKPTNTLREYCGSGCTAGIGYVVTQGSGSQQAAQRAALGLGFSDNASAETMAHE
                     VGHNHGRSHAPCVQGGTISGVDAAFPQKDGTIGVYGWDLRKSALIPPTYTDMMGYCSK
                     QWISDYTYKGILNRVASLNTTAMASEVVRSELIQPYRVLLIEPKGARWGIPISEPALP
                     SGEPELADLLDATGQTVSQVEVYRTAISDIEAFSVEVPEPPAGVVAIRVAGAAEILF"
     gene            complement(3996..4856)
                     /locus_tag="VFQ61_10870"
     CDS             complement(3996..4856)
                     /locus_tag="VFQ61_10870"
                     /inference="COORDINATES: protein motif:HMM:NF019274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HET9954999.1"
                     /translation="MAKLNSGGWSKSLHQLGGMSLSLSTGGLLLSLLMGGCSGGPPPQ
                     SCWRDGTEYKSGSPVPSGDCNTCSCSDGQVVCTLIFCVDPPPRSCGGLLGAGCEPGEF
                     CNYEPGQMCGAADQTGTCQAVPEICPDIYSPVCGCNDRTYPNACYAARDGVGVSAEGE
                     CAPPAGSCEVDGVIYPDGASGIPAPDGCNTCFCSNGSLACTKIGCPPKKGCGGRLGNT
                     CAKDEYCAYVNGDCGRADGTSTCETRPDACTREYRPVCGCDGKTYSNLCTAASAGQGT
                     LHEGPCESVD"
     gene            5112..5957
                     /locus_tag="VFQ61_10875"
     CDS             5112..5957
                     /locus_tag="VFQ61_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9955000.1"
                     /translation="MNHSIQNSHYTFGDTDLAARRLVILSGVFDPYSREFITQWAPVQ
                     PRFAIDLGCGPGATTRLLHEASGAQSTLGIDSSQNHLARARERAVPGLEFECHDLREH
                     PYSFGPAELIFARFILTHLADPAHTLAGACARLQSNPAGGPSRLLVQETSRMESEYPI
                     LSRYYELVFELQRRVGQAMYIGREFGDIAERALREAETAMGPSVRIAHVSERRFEVEA
                     RNMAELHLMNLRTWREDPVAKAAFDADELDHLDRGLSDILHGQVTVPPVQLGLGELVL
                     ERVRD"
     gene            6057..>6222
                     /locus_tag="VFQ61_10880"
     CDS             6057..>6222
                     /locus_tag="VFQ61_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9955001.1"
                     /translation="MDLTDDQWEVVRKFIPGPEKMRTSEVGGRPWRDPRDVLNGVLWV
                     LRTGAPWADLP"
BASE COUNT         1159 a         1960 c         1928 g         1175 t
ORIGIN      
        1 actaccgcta ggttgtgaca gccgacttcg ctgttgcccg aacaggcgcg gtcgaacaag
       61 ccctgagcac gcggaagatc tttgggcaca ccccggcctt cggcgtagag cgcgccgagg
      121 ttggagcagc cgagcgcgtt gccgctgtcg cacgcacgca ggtacatttc tctcgcgcgg
      181 gtgaggcttt gcgggatgcc ccagccgttc tcgtacatca ctccgaggtt gctgcagcca
      241 tcgccgctgg aaccgtcaca agcttttcga aacaacttcg cagctttggc gtccaggtcc
      301 tctccgaact cggagcgcgt gttctcgtac acgacgccga ggttgttgca gccaatcggg
      361 ttgcccgcgt tacacgcgcg atcgaaggag acccgagctg cctgtaggtt aggaccggag
      421 aagccctcgt gttgaatgat ctcgaccccg agcgcgttgc acacggttcc ttcacccgca
      481 atgcacgctt gaatacgttg cgttgcgtcg agtttcggcg cagaagactc cgccgcgtgg
      541 aaagaattgc gcggcaaacc cgcggtcgga tcggcagtga cggaactcga ttgtcgaaaa
      601 ccgccgacgg tcaggcctat caaggccaac aacgctagcc cgccaagccc gacgcgatcg
      661 cggtgagcgc ggcgagccgc cgattctggc ccggcaaaca ggctctcttc acccgtttgc
      721 gcagggacac ttcgaacaaa cgtggtgtcg aactccaaat gggagctggc acttcgctca
      781 ccggcatcgg tgcaaagtga atgtctcatg ggaacctcac ctcagagtgt gagcatgcgt
      841 cgtgccatgc tctgccctcg ctactctcgg tctactgtga gtgcgaactc gagaatggcc
      901 cgagttcgcc caaaccaagt ttacgggaga atgtgaggtg cctttacccg gtgagtgccc
      961 ggaaaaccgt gtgcgctcga attagaagag aatttcggca gcgcccgcga ctcggatcgc
     1021 aacgacaccc gctggtggct ccggcacctc gaccgagaag gcctcgatat cggaaatcgc
     1081 ggtacggtaa acctcgacct gcgacacggt ttggcccgtg gcatccagca aatccgcgag
     1141 ctcgggctca cccgagggaa gagccggctc gctgatggga ataccccaac gcgcgccttt
     1201 gggctcgatg agcagcacgc gatagggctg aatcaactcg ctgcgcacca cctcggaagc
     1261 catggccgtc gtgttgagcg aggccactcg gttgagaatg cccttgtacg tatagtcact
     1321 gatccattgt ttggagcagt aacccatcat gtcggtatag gtcggcggaa tgagggcaga
     1381 cttacgcaag tcccaaccgt atacgccaat cgtgccgtct ttctggggaa aggcagcgtc
     1441 cacgccgctg atcgtgccgc cctgcacgca gggagcgtgc gagcgaccgt ggttgtgacc
     1501 cacttcgtgc gccatcgtct ctgccgaagc gttgtcgctg aagcccaaac cgagcgcggc
     1561 gcgctgggcg gcttgttggc tgcccgagcc ctgggtcacc acgtaaccaa tgcccgccgt
     1621 acagccactg ccgcagtact cgcgcagagt attcgtaggc ttgagcaggc cgtagtagta
     1681 gatgtcggcg gccggggcgt cctggcggcg cttggcccga acctgatcca gcatcgtggt
     1741 ccaatctttg ggatcggcca cgctgaggga gtctcccacg gtgatctgga cgtcggtgac
     1801 gggatagatc gccatcactt cgtcccggta gatttgaagc gcggcctcgg aagtgtccgg
     1861 tttgttgctg ccggaggtga gggggatcac gcgaaccttc accacaccgg tcgtgcgagc
     1921 ctcgagtggg gtgctcgaag tcgcggggaa cttggggctc tggaccgcac ctccgggagc
     1981 gcctccacac tcgaccaact ccacctgata gcgggttgcc gtggtgatct tttccttcgg
     2041 tactttgacc tgaatggtcg agtcgagcgt gccctcttgc gaagagccag cgagcgactt
     2101 cttcgtgtaa aacacgtcca cctggccgtt gttctcgacc agcacgcgcc ccgagatatt
     2161 gcgcgcgacc caaccgctgc cgaccgtcgc gaacaacctg aacatggctt cgcggcccgc
     2221 caccacatcg gtattgcgag ctgcggcagc caccgcctgc cccgcgttca tgactcggac
     2281 gcgcacggat tggaacacat cgacctgact caacgtgacg ttctgtgcga ggccactaca
     2341 gccgtcagga cctgcggctc cgccgttgct ctcgacacat tgcccggcag tgcagctctg
     2401 acccaaggca cacggtgtgc cgcaaccacc gcagttttgg aggtccttca tcggatcgac
     2461 gcacgctgcg ccgcaacgga gctggcccgc cgcgcattga caaagtccgg ccgcacacgc
     2521 gagcccggcg ccgcaggggg tgccgcacgt tccgcagttc gccgcgtcgg tttggaggtc
     2581 cacgcatttg cccgagcaca ccgcgcgtgg tgccgcgcaa gtgcattggc ccgcgctgca
     2641 cacctggtct gcgctgcatg gccgcccgca ttgtccgcaa ttctgggcat tgctctgcag
     2701 atccacacag cttcccgagc agctggcttg cccagcctga cacgcgccag caccagccgc
     2761 ccccgccgtc gccgtgctgg agccgcccat gccggttgtc agcgtgccgg cggcagcgcc
     2821 gccctgggcc gcgctgcctc cgctgccgat tgctccaccc tgcggcgccg ctcccgcgaa
     2881 tccgccgcga tcaccgccgg agagcgcgcc tccgatgcca gccgcgccgc ccatgggatt
     2941 gaccggaaag ccgccggaac cgggcagatt gccagtatca tcgatgcttc cggaacagcc
     3001 catcaagggg ctcgcgacca aaacgggtgc ggtcagcaac caccaactag cgcgagctac
     3061 acgcgctgaa tctggcttcg cgaaatctgg ctgcgtcgcc gcattggatc gcgcaaactg
     3121 gaccgtagtt ccggacgcga aaaatctgga cgcgaaaacc ttcgacgcac gcaaccctgc
     3181 gagccgtacg gagcgcttca tgtgggcgtc agtgtagcgc tgacgctagc cgtgcgctcg
     3241 tgattctgat gctcggcgag tgcgaatcga gctcacttat cccgcggtgg tcgcatgagc
     3301 ctggtggcgc gaatgggcga tgcggacgct cctttcggaa tgttcgaatc agcctaaaat
     3361 ccctgcattc tcagagggaa tcttccttaa ctcaccgtca cggcagtcac cccgaagagg
     3421 gacacaaacg cggatatgcc ttcagttgca ctcacgcttt tgcgtgtgcc tggtcgtgcc
     3481 gtaattcggt tacccggatc ggctgcatcg cggagcgcgc acgcggagcc gtggaaattg
     3541 cggggcgcgc acgcggagcg gtgggaatca cggagcggtg gcactgccta gcgccagcgc
     3601 tgccaagcag tgggctgcac ggcatcactg cattcggcca acacaaagca gtttattagg
     3661 tggcggtgag ttggttagga acgcgagacg gaggcgcaag acgcgcatgc gaccttcacg
     3721 agaagcgccc gcgaaaattg cgagccaccc atttcgcgca cttctggcac ggcgaagctc
     3781 gagagagctt ccgacatgac gctcggactt cggctcactc gacgtgacag agatcggaca
     3841 cgtggcatgg ggcgccaccc gccagtcagc agttttcgaa ctggcgggtg tcatgcccgt
     3901 gcgctcgcgc tcggtgcgtt cttggctccg tacgccgttg ttatgagcgt tctcggctcc
     3961 gtatcgggtt cttcgctacc cctcttgggg ctgcgtcaat cgaccgactc acacgggccc
     4021 tcgtgtaagg tgccctgtcc ggcggaggcc gcggtgcaga ggttcgagta ggttttgccg
     4081 tcgcaaccgc ataccggcct gtattctcgt gtgcaggcgt cggggcgcgt ctcgcaggtc
     4141 gaagtgccat ctgctcgccc acagtcacca ttcacgtagg cacagtactc gtccttcgcg
     4201 caggtgtttc caagtctacc gccgcagccc ttcttgggcg ggcacccgat cttcgtacaa
     4261 gccaagctcc cgttggaaca gaagcaggtg ttgcagccat cgggcgcagg aatgccggaa
     4321 gcaccatccg ggtagatcac gccgtcgact tcgcagctac ccgcgggagg tgcacactcg
     4381 ccttcggcgg acacgcccac gccgtctcga gctgcgtagc aagcgttggg ataggtgcga
     4441 tcgttgcagc cgcacaccgg tgaatagata tccgggcaga tctccggaac ggcttggcag
     4501 gtgccggttt gatcggcggc gccgcacatc tggccgggtt cgtagttaca gaattcccct
     4561 ggctcgcagc ctgcaccgag caagcccccg cacgatcgcg gcggcggatc cacacagaaa
     4621 atgagggtgc aaacgacctg cccatcactg cagctgcagg tattgcagtc cccggaagga
     4681 actgggctcc cgctcttgta ttccgtgcca tccctccagc aggactgcgg cggcggccca
     4741 ccgctgcacc cgcccatcag cagggagagc aagaggccgc ccgtcgagag gctcaggctc
     4801 attccgccga gttgatgtaa agacttcgac caaccacctg aattgagttt cgccatgcac
     4861 gaaggctaac agctttgagg ctcggcgagc ggtctccgat tgaccgatcg ccgagactaa
     4921 gttaaagaca gcgctccgat gacgaactcg ccccgcgcaa cttatccgga tcgccacatg
     4981 tggtggccgg cgttcgctgg gcgatcgctg aatcatcggt aacctaaagc actggtgaag
     5041 gaagcgagca gcccgcgaga gctgctcgtg atttcaggca cttgcagcgt ttgcgggcgc
     5101 gaacgagagc catgaatcat tcaatccaaa acagccacta cacctttggg gacaccgatt
     5161 tggcggcgcg tcgcctagtc attctatcgg gcgttttcga cccgtactcg cgggagttca
     5221 tcacgcagtg ggcgccagtt cagccgcggt tcgccatcga cttggggtgc ggtccgggcg
     5281 ccaccactcg cttattgcac gaagcgagcg gcgcccagag cacactaggc atcgattcat
     5341 cgcagaatca cttggctcgt gcgcgcgagc gtgccgtacc aggcctagaa ttcgagtgcc
     5401 acgacttgcg ggaacacccg tattcgtttg gtccagccga gctgattttt gcgcggttca
     5461 ttctcactca cttggcagat cccgcccata cgttggcggg agcctgcgca cggctccagt
     5521 ccaatccagc cggtgggcct tctcgcctcc tcgtgcagga gacctcgcgg atggaatcgg
     5581 agtatccaat tctgagccgc tactatgagt tggtcttcga gctgcaacgc cgcgtggggc
     5641 aggccatgta cattggtcgc gagttcgggg acatcgcgga acgagctctg cgggaggccg
     5701 aaacagcgat ggggccgagc gtccgtatcg ctcacgtcag cgaacggcgc ttcgaggtgg
     5761 aggcgcggaa catggccgaa ctgcacctga tgaatctgcg gacttggcgt gaagacccgg
     5821 tcgcaaaagc agcgttcgac gcagacgagc tcgatcacct ggatcgaggg ttgtcggaca
     5881 tactgcatgg gcaagtgacc gtgcctccag tccagctggg gctgggcgag ctcgtgctcg
     5941 aacgcgttcg agactagagc ccatctcata acctcgcagc gtgcaagtga tcgattttgc
     6001 tccatctttc acgagggtct ttccattggg ctggaaacct gtttgtaagt tcgttgatgg
     6061 acctgacgga cgatcagtgg gaagtggttc ggaagttcat tcccggcccg gaaaagatgc
     6121 gcacgagcga agttggtggc cggccgtggc gagatccacg agatgttctg aatggggtgc
     6181 tttgggtctt gcggacgggc gcgccatggg cggatctgcc gt
//
LOCUS       DASTKU010000304         4058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_506150, whole genome shotgun sequence.
ACCESSION   DASTKU010000304 DASTKU010000000
VERSION     DASTKU010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506150"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFQ61_10885"
     CDS             <1..492
                     /locus_tag="VFQ61_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="low-density lipoprotein receptor class A
                     repeat-containing protein"
                     /protein_id="HET9955002.1"
                     /translation="GMGTAGSGAGGASFVPGDFECPDGSRIGPERVCDARPDCSDNSD
                     ESARICGEQPPPFICSNGQVAASDAWCNGIIECQDRSDEDAMRCQGASYFTCASGERL
                     FASSVCNAVLNCRDGSDESPALCRNITAPLFRCEDGRALDPSQLCDGVNDCASGKDEY
                     CGK"
     gene            640..1359
                     /locus_tag="VFQ61_10890"
     CDS             640..1359
                     /locus_tag="VFQ61_10890"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HET9955003.1"
                     /translation="MQEALAFRCPICGGPTEAAARRCEYCRSALATLRCAACFHLNPG
                     NAKHCGGCGFELGLEPLATTSTLHCPSCSAAMEALSEAAGTLYVCGSCEGQFVEHRLM
                     SALVERQERVQTELVTGPPRAKHIVGSVAYRACPICSALMWRKNFGARSGVIVDVCAR
                     HGSFMDPGELPDILTFVSRGGLERERREKADQARKKDVALRALPESRDPQNPLELSLS
                     ELGLADVVQFFRNLLGRGPGQ"
     gene            complement(1333..2100)
                     /locus_tag="VFQ61_10895"
     CDS             complement(1333..2100)
                     /locus_tag="VFQ61_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955004.1"
                     /translation="MTSDREPRRWLEDASSDPELRELLQAGCKEEPSRGAIRAAPAAI
                     AALLSTHAVSGAAAAAVATKGAASLAGASGVATTGLVVKWVAVGALIGTSVSMAAFTI
                     KSSPESSSRSKGAIAQVQSVPARETTHERVAPPVSGDAVPAPAASSPVAMPEPSGGGR
                     PNSRADLSREVSELDAARAALARGHAEQALLILNAMELLPNRALVPEASVLRVRALLR
                     VGQREQAERVAERFLERAPNAPQSRVLRALLAGTASE"
     gene            complement(2097..2675)
                     /locus_tag="VFQ61_10900"
     CDS             complement(2097..2675)
                     /locus_tag="VFQ61_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9955005.1"
                     /translation="MTLPHAFNTPPSEADAPLERVASTEQIREVVEREYAVVWRFLRR
                     LGVPEAHVDDAAHSVFARVLAKDSVILAGAERAYLMKAAFHISFEYRRSQKRAYARSS
                     AQDADELSSREPLPDELLAQRERRRMLDAALQRLSPELRAVFTLFELEGLSFTEISQA
                     LDIPRGTVASRVRRARELFTEAVQRLTRGALP"
     gene            complement(2754..3881)
                     /locus_tag="VFQ61_10905"
     CDS             complement(2754..3881)
                     /locus_tag="VFQ61_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955006.1"
                     /translation="MSVRRWLLRCGWVSRFGRRSRRDAGLVVGAAIFWAASAASATEV
                     QARVEWLGVCGNPEELARETSQRGVELTDGAPAGTLAPEVVRVQVQVTERRGSHPLAA
                     ELVLEDPQGGHEQRSLEARSCGELRRGVAWMLVTLAREAAAPSRGPGQGPPADSAWSA
                     PSTSPAFPDLHSGPQPSSRPMMPCPPPPAPHGRSLWAPRQPRGRSTTGDAARLGFALV
                     TGFGFADRPSLGPLLYLDREIESLNLRLKLSAFQLSTLPFRADAATLSIDRLAMRFGA
                     HWSPPALPTLSLGLSAEAGRLIAAGSGLKVSRTDAASWFALEPAVVLRWPLFRKLLWL
                     DLGLSAAYTPFKYQFEYGTGQRVSQAGPFEARFQAGLSVEL"
BASE COUNT          765 a         1411 c         1234 g          648 t
ORIGIN      
        1 ggaatgggca ccgcgggttc tggcgcgggc ggcgccagct tcgtccctgg tgatttcgag
       61 tgccccgacg gcagccggat tggtccggag agagtctgtg atgccagacc cgattgctcc
      121 gacaacagtg atgagagcgc caggatctgc ggagagcagc ctcctccgtt catttgctcg
      181 aacgggcaag tcgccgcgtc ggatgcttgg tgcaacggca ttatcgagtg tcaggatcgc
      241 agtgacgaag acgcgatgcg ctgccaaggc gcgagctact tcacctgcgc gagcggcgag
      301 cggctcttcg ctagttcggt gtgcaacgcg gtcttgaact gtcgcgatgg cagcgacgaa
      361 agtccggcgc tgtgccgcaa tatcacggct ccgctcttcc gctgcgaaga cggccgtgcg
      421 ctggacccga gtcagctgtg cgacggcgtg aacgactgcg cgagcggaaa agacgaatac
      481 tgcggcaaat aaccggtctt ccaggcccgc gccatcgggc cctggagctc tcgcgcccta
      541 cacgtagcct acagtgagag gccgtgatcc tgcgtattcg acgtcacctg acgtttgccg
      601 cccagcgctc gacgcttcaa ggtgtgctcg agatcgctga tgcaagaagc cctggccttt
      661 cgctgcccga tttgtggggg gcccaccgaa gccgccgctc gacgctgcga gtactgtcgc
      721 tcggccctgg cgactttgcg ctgcgcggcc tgcttccact tgaacccagg caacgccaag
      781 cattgcgggg gctgcgggtt cgagcttggc ctcgaacccc tcgccaccac ctccacgttg
      841 cactgcccta gctgcagtgc agcgatggag gcgttgagcg aggcggcggg gaccttatac
      901 gtctgcggca gctgtgaggg acagttcgtc gagcaccgtt tgatgagcgc cttggtcgaa
      961 cgccaagagc gcgtgcaaac ggagctcgta accggcccgc cccgcgccaa gcacattgtc
     1021 ggttcagtcg cgtaccgcgc gtgtccgatc tgcagcgcgc tcatgtggcg gaaaaacttc
     1081 ggcgcccgga gcggcgtaat cgtcgacgta tgtgcgcgcc acggctcgtt catggacccg
     1141 ggagagttac ccgatatcct cactttcgtg agtcggggtg gcctggagcg cgaacggcgc
     1201 gagaaagcgg accaagcccg caagaaggac gttgcgctgc gcgctttacc ggagtcgcgc
     1261 gatcctcaga acccactcga gctcagtctc tccgagttgg gcctcgccga cgtggtgcag
     1321 ttcttccgaa acctactcgg acgcggtccc ggccaatagc gcccgcagca cacgcgactg
     1381 aggtgcattc ggtgcacgtt cgaggaagcg ctcggccacc cgctccgctt gctcgcgttg
     1441 ccccacccga agcagcgccc gcacgcgaag cacactcgct tctggcacca gcgcgcgatt
     1501 gggcagcagc tccatcgcat tcaggatgag caacgcttgc tctgcatgcc cgcgcgcgag
     1561 cgcggcccga gctgcatcca actcgctgac ttcacggctc aaatcagccc tggagttcgg
     1621 cctaccgcct ccactcggtt cgggcatcgc tacgggcgag ctcgccgcag gcgccggcac
     1681 cgcgtcaccg gagacagggg gggcaacacg ctcgtgagta gtctctcgag cgggaacgga
     1741 ttgaacctgc gcaatcgccc ccttcgaacg ggacgaagac tcgggactgg acttgatcgt
     1801 gaacgcagcc atcgaaacag atgtcccgat cagcgctcct accgccaccc atttcactac
     1861 cagcccggtg gttgcgacgc cgcttgcgcc agccaggctc gcggcccctt tggtggccac
     1921 cgccgctgcc gcggcgcccg agactgcgtg ggtggaaagc aacgccgcga tcgctgccgg
     1981 agctgcgcgg atcgcacctc ggctgggctc ctccttgcac ccagcttgca gcaactcccg
     2041 aagttccggg tcactcgagg cgtcctcgag ccagcgtcgg ggttcgcgat cggacgtcat
     2101 ggcaaagctc ctcgcgtcag ccgctgcact gcttccgtga acaactcgcg tgcgcgccga
     2161 acgcgcgacg ccacagtgcc tctcggaatg tccagcgcct gcgatatctc cgtaaaactg
     2221 agcccctcta gctcgaacaa cgtaaacact gcgcgcagct cgggcgacaa ccgctgcaac
     2281 gccgcatcca gcatgcggcg gcgctcgcgt tgtgccaaca actcatcggg cagcggctca
     2341 cggctgctca gctcatccgc gtcttgcgcg gaggaccgtg cgtaagcccg cttttgactg
     2401 cgccggtact cgaaggagat gtggaaggcc gccttcatca agtacgcccg ctccgcgccg
     2461 gccagaatca ccgaatcttt ggccagcacc cgtgcaaaca ccgaatgagc ggcgtcgtcg
     2521 acgtgcgcct cgggcacccc caagcgccgc agaaatcgcc acaccaccgc atactcacgc
     2581 tcaacgacct cccgaatctg ctccgtcgat gccacacgct ccagcggcgc atcggcctca
     2641 ctgggaggag tgttgaacgc atggggcaag gtcatccggt atgaccaagc tccgagtgga
     2701 ccattcggat caactcaacc acgagaacgc acaaatgcta ggcccaccgc gcattacaat
     2761 tcaaccgaca gcccggcttg gaaccgcgct tcgaacggcc cggcttgaga cacgcgctgc
     2821 cccgtcccgt attcgaactg atacttgaac ggagtgtagg ccgcgctcaa gcccagatcg
     2881 agccaaagca acttacgaaa caacggccat ctgagcacga cggctggctc cagcgcgaac
     2941 caactcgccg catccgtgcg cgacaccttc aaccctgaac ccgctgcaat caagcgaccc
     3001 gcctctgcgc tcaagcccaa actcaaggtg ggcagcgcgg gagggctcca gtgagctccg
     3061 aaacgcatcg ccaaccggtc gatggagagg gttgccgcgt ctgctcgaaa gggcaacgtg
     3121 gagagctgaa acgcgctgag cttgagccgg aggttcaagc tttcgatctc acgatccaga
     3181 tacaacagcg gtccgagtga gggtcgatcc gcgaatccga aacccgtcac caaagcgaac
     3241 cccagccgtg ccgcatctcc ggtcgtcgaa cgcccgcgcg gctggcgtgg tgcccacaaa
     3301 ctcctgccat gaggagcggg cggaggtgga cagggcatca tcggtctaga agaaggctgg
     3361 ggtccggagt gcagatctgg aaaagccggt gacgtcgaag gagcactcca ggcgctgtcg
     3421 gcgggcgggc cctggcccgg accgcgcgac ggcgcagccg cctcgcgagc caaggtcacc
     3481 agcatccagg ccactcctcg gcgcaactct ccacacgaac gcgcctccaa actccgctgc
     3541 tcgtggccac cctgcggatc ctcgagcacc aactcggctg cgagcgggtg cgagccacgc
     3601 cgctcggtaa cctgcacctg cacccggacc acctccggag cgagggttcc ggcgggcgcg
     3661 ccgtcggtca gctccacccc ccgttgactc gtctctctcg ccaactcctc gggattcccg
     3721 caaaccccca accactccac ccgagcctgc acctcggtgg ccgacgccgc cgaagccgcc
     3781 cagaagatgg ccgccccaac gaccagaccc gcgtccctcc gagagcgccg cccaaaacgg
     3841 gagacccagc cacaacgcag aagccaacgg cgaacagaca ctttacccgg tattagcccg
     3901 agcaactcca gaacccaacc cgatcgcacc cgccggagaa gcgcccttca ggtcccgtac
     3961 gaggttggtg gttccgtcgg tcgtcaggtc cgtcattccc ggtccggtca ggtgagggcc
     4021 aggtacggcc agatccggta cgaccttgcg tttgtcgc
//
LOCUS       DASTKU010000305         6485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1174985, whole genome shotgun sequence.
ACCESSION   DASTKU010000305 DASTKU010000000
VERSION     DASTKU010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174985"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(529..1824)
                     /locus_tag="VFQ61_10910"
     CDS             complement(529..1824)
                     /locus_tag="VFQ61_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955007.1"
                     /translation="MRFQGLLPSALLVAVCSSVVGCQPQGPVRAALKEDLPSLRAEIL
                     AAEARGKLDRKSVLSVAEAVAQREAASANGPTGVEHVHALRGCAKPLLGVLRSLSERH
                     DEVGAEALLARIQQGDVQLASLTKYANDADPAFRAVAARGLIRAADGPARRRSFTDPD
                     QRVRRAALEAARVAHDAYDLEGLLEVVRLDPDSLSRSWAARAIGAIGGEQAVLGLKDR
                     YERADAVTKLAILEAWAAPATLATGGERELARIAAQPSGIAALTAAQLLRTQPLSKDR
                     ARDGAWSTRIRVGIENGTSDEKRVAIGFAELSDPATVKAVEAAARDLDSSVQLTALRR
                     LTELPATRNAAFARLRSLAQGKTPEAAEVQLTLAELGERSVLPVIEQTLERGPSTLRS
                     RAALALFGIGREARAARALADADPSVRISVSCGMLARSR"
     gene            complement(2060..3733)
                     /locus_tag="VFQ61_10915"
     CDS             complement(2060..3733)
                     /locus_tag="VFQ61_10915"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET9955008.1"
                     /translation="MSRRTKFVLVTGGVVSSIGKGLTSASIGALMEARGLRVTHMKLD
                     PYINVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTTCRLSRQANVTTGRIYEAVI
                     SKERRGEYLGATVQVIPHITDEIKARIHEATKGADIAIVEVGGTVGDIESLPFLEAIR
                     QLKLEAGTENALSVHVTLVPHIVTAGELKTKPTQHSVREMREIGIQPDILICRSDRPI
                     ARGLKEKIALFSNVPVDSVISAVDVSCIYELPLVLHAEGLDEQIAERLNIWSREPEIG
                     AWKRTAEKFKKPANGSVRIGVVGKYVHLKDSYKSLHEALVHGGLANDVAVQLEYIDSE
                     QLERGSAAALLSGLDAVLVPGGFGDRGAEGKISAIRYAREHQVPFFGICLGMQLAVIE
                     VARNVCGLKGASSTEFDKESPHPVIDLMPDQRGVRDKGGTMRLGSFPCALKAGSRAFE
                     IYGKEEISERHRHRFEFNNEYREQLMRAGLVLSGTSPDDRLVEIVELPGHPYFVGCQF
                     HPEFKSRPTAAHPLFSSFVRAAVRRRDEIARSARGEGSSPGHPSESTVN"
     gene            complement(3862..4524)
                     /locus_tag="VFQ61_10920"
     CDS             complement(3862..4524)
                     /locus_tag="VFQ61_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955009.1"
                     /translation="MKRATSFACALLLLWEGVARADDPQASPPAGAGARPATAPSLGA
                     APTQTPIPAPASAGSDPMALPPSSSSASSAAAAEPDALPPPSARPLVLLAGVGTFALW
                     YGAALAQSYAWPKADEADKLRIPVAGPWMTLAHAGCSDEEPKCTTVLGVVRGALAGLS
                     GIGQVGGLALLSEALFLRTGSSGSQSALETRSSAPIHASVKGVTFATERDGLRVGVYG
                     SF"
     gene            4567..6276
                     /gene="dacB"
                     /locus_tag="VFQ61_10925"
     CDS             4567..6276
                     /gene="dacB"
                     /locus_tag="VFQ61_10925"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HET9955010.1"
                     /translation="MRRRRVSLEVATTFEAVLSYGVPFRDDTSVQANIFRHLSEDCQA
                     LDIQRGPQAAVRATAASFSPGVRWSAPCSQAVLSTASRSPAALSRRALLAGTFAFSTG
                     AFQRSALAQAASVSDALRASFDKLVSWAQRQRVALGAAVLEVSNGQPLLASGELLPLN
                     PASNQKLLTMAAALERLGPAQRFSTSLHGRLTGDTLSELVLRGDGDPELTVTRLERMV
                     RSLADQGVRRVAGDVLVDQSAFDARWDPPAYEQRPRDEAAYRAPVSAVALDGNLLVLH
                     VFPSRVGASGEPSPARLWLEPPGLAQIEGSVSTVSSSPAGVSFSVLSKDGKLVARVGG
                     SVLASGGEVSFSRRLPEPELAAGRVLLRLLSDHGIVVSGRLRSGGAEIQSELVALRSR
                     SLGELLLPLGKQSDNFAAEMLLKALGARSTASSGTSAAGAAVLMDYLQKRGLLLPGTT
                     ITNGSGLFDANRLTALALARVLREAYLDPRSGPEFLAALSIGGTDGTLSHRFGALNAS
                     RRVRAKTGTLSDVIALSGFVFGSSMASARCFSFLLNGVSGHQTEAKNLVDRVVLALAA
                     EAP"
BASE COUNT         1111 a         2171 c         2031 g         1172 t
ORIGIN      
        1 ccaatcacga cccgagacgc tggattttgc gccacttcga cgctagccgt tcgctcgagc
       61 cagttcgccc gtaagccagt ccgttggtga gcctctcgcc cgcgaaccga gccgcgacgc
      121 gtgcatcatg cctcgtaggt aacgagcttc ccattggagt ggagctccca gcggcgctgc
      181 tgtaggtgtt atggagcttg gcagcggcgt ttctgatcgg cgttcttcga gactcgattt
      241 gctgtagtcg gtctgcaagg tagcttcccg caacgaatca gcgcctgcgc tcatgtgctc
      301 gaagagtgcg gaggtcgacg ctcgcgcggt tcgcgtgtac ccctgaggta tagagcgggt
      361 cagttggcga gaggcgctga cgcactgggc gcagcgcccc aggtgcggct cgctacacac
      421 gtcgttcatc tcagttggcc cagtcgcgca aaaccggagc aacttgcgca ctggtaaccc
      481 tccgcacgcg gctcccaagc cacggccgcc cggttcgaaa attcgagttc agcgcgaacg
      541 ggccagcatg ccgcaggata cgctgattcg aaccgacggg tctgcgtcag ccaatgcccg
      601 agcggcgcgg gcctctcgcc cgatcccgaa cagcgccaac gccgcccgcg aacggagcgt
      661 gctcgggccg cgttccaacg tctgctctat caccggcaaa acgctgcgtt cacccaactc
      721 cgccaaggtc agctgcacct ccgcggcttc gggcgttttg ccctgagcga gcgatcttag
      781 cctggcgaag gcagcgttcc gagtcgctgg caactccgtc agcctacgca aggccgtgag
      841 ctgcacactg ctgtccagat cacgagccgc ggcctcgacc gccttcaccg tcgccgggtc
      901 tgaaagctcc gcgaacccga tcgcgacccg cttctcgtcg gaggtgccgt tctcgatacc
      961 gacacggatt cgagtactcc acgctccgtc gcgcgcacgg tccttggaaa gtggctgagt
     1021 tcgcaagagc tgtgcggccg tcaaagccgc gatgccgctc ggttgcgccg cgatccgagc
     1081 gagctctcgt tcgccccccg tcgcgagcgt ggccggcgcg gcccatgcct ccaaaatagc
     1141 gagcttcgtg acggcatccg cgcgttcgta gcgatccttg agccccagca cggcttgctc
     1201 cccgccaatc gcgccgatcg cgcgagccgc ccagctacgg ctcaacgagt ccggatccaa
     1261 gcgcacgacc tcgagcaatc cttctaaatc gtatgcatcg tgcgcgacgc gcgctgcctc
     1321 cagagccgcg cgacgcacgc gctgatcagg gtcggtgaac gagcgcctgc gtgcagggcc
     1381 gtctgccgcg cgaattagcc cgcgtgcggc cacggcgcgg aacgccggat cggcgtcgtt
     1441 cgcatacttc gtcaggctcg cgagctgcac gtcgccttgc tggatgcggg cgagcagagc
     1501 ttcggccccc acctcatcgt gccgttcgga cagacttcga agcacgccca agagcggctt
     1561 cgcgcaccca cgcagcgcgt gcacgtgctc gacacccgtg gggccattcg ccgaggccgc
     1621 ttcgcgctgc gcgacggcct cggccaccga gagcacgctc tttcgatcga gcttaccccg
     1681 cgcctcggcg gcgagaatct ccgcgcgcaa gctcggcagg tcctctttga gcgccgcacg
     1741 caccggcccc tgtggttggc acccgacgac cgaggagcac accgcaacga gcagcgcaga
     1801 cgggagcaag ccttggaacc gcattctttc gcacctatcg ttttggctga gaagctagtg
     1861 ccgcctcatc tgattttcga gccctcgcag ccgtccgata cgcgccggat gagcccctcc
     1921 aatcttcgcg attcgaccgt gtgcgatgaa ttggccaacc gaattggtca acctgggggc
     1981 gttgacggat tcgtgcgcgc cggataggcc gagcttgtct agccagcgct gcttgcgaac
     2041 ccgtttcggc ctgaagggat cagttgaccg tgctctccga cgggtgaccc ggtgaggagc
     2101 cctcaccgcg cgccgaacgc gcgatttcat cgcggcggcg caccgcagct cgcacgaagc
     2161 tcgagaagag ggggtgagca gcagtcggcc gactcttgaa ttccgggtgg aactgacagc
     2221 ccacgaagta ggggtgaccc ggcaactcga cgatctcgac gaggcgatcg tccggactgg
     2281 tgccgctcaa caccaggccc gcgcgcatca actgctcgcg atattcgttg ttgaactcga
     2341 agcgatgacg gtgccgctcg gagatctctt ccttgccgta aatctcgaag gccctgctac
     2401 ccgccttcaa cgcacacggg aaggagccca ggcgcatggt gccgcctttg tcgcgcacgc
     2461 cacgttgatc cggcatcaga tcgatcaccg ggtgcggtga ttctttgtcg aactcggtag
     2521 agctcgcgcc cttcaagccg cacacgttgc gtgccacttc gatcaccgcc agctgcatgc
     2581 cgaggcaaat tccgaagaac ggaacttgat gttcacgcgc gtagcggatc gcggagatct
     2641 tgccttccgc accacggtcg ccaaaaccac cgggcaccag caccgcatcc agaccactca
     2701 gcaaagcggc cgcgctgccg cgctcgagct gctcggagtc gatgtactcg agctggaccg
     2761 ctacgtcatt ggcaagcccg ccgtgcacga gcgcttcgtg caaagacttg tagctgtctt
     2821 tcaggtgcac gtacttgccc acgacgccga tgcgcaccga accgttcgcc ggcttcttga
     2881 acttctcggc ggtgcgtttc caagccccga tttcgggctc gcgcgaccaa atgttcaaac
     2941 gctccgcgat ctgctcgtcc aaaccttcgg catgaagcac cagcggcagc tcgtagatgc
     3001 aggagacgtc caccgcggaa atgacgctat ccaccggaac gttggagaac agcgcgatct
     3061 tttctttcag gccgcgcgca atcgggcgat cgctgcgaca gattagaatg tcgggctgga
     3121 taccgatctc gcgcatctcg cgcaccgagt gctgcgtggg cttcgtcttc agctcgcccg
     3181 ccgtcacgat gtgcgggacc agcgtcacgt gcacactgag tgcgttctcc gtgccggctt
     3241 cgagcttcag ctgacgaatg gcctccaaga acggcaagga ctcgatgtca ccgacggttc
     3301 cgccgacctc gacgatcgcg atgtccgcac ccttggttgc ttcgtggatg cgcgctttga
     3361 tctcgtcggt aatgtgcggg atcacctgca ccgtcgcgcc gaggtactcc cctcgccgct
     3421 ccttgctgat cacggcttcg tagatgcgcc cggtggtcac gttggcctgg cgactcagcc
     3481 ggcaagtggt gaagcgctcg taatgaccta gatctagatc cgtctcggcg ccgtcatcgg
     3541 tcacgaagac ttcgccgtgc tgatacggcg acatcgtgcc cggatcgacg ttgatatacg
     3601 gatccagctt catgtgcgtc acccgcaacc cgcgcgcctc catgagcgcg ccgatcgagg
     3661 ccgacgtgag acccttgcca atggaggaga cgactcctcc ggtgaccagg acgaacttgg
     3721 ttcgtcgact caccggcata cctccgtcac gcgcaggtgc gtaccacgcc tctgcaggcc
     3781 gcgcatccca ttcgaagcat cgttccagga tttcaatgtc acggtctcac tctttcgata
     3841 aagcacgcac gcggcgcgcg atcagaacga accgtacaca ccgacgcgca gcccatcgcg
     3901 ctcggtagca aatgtgacgc cttttacgga agcgtgaatc ggcgcactgc ttcgcgtctc
     3961 gagcgctgat tgtgacccag atgagcccgt gcgcaaaaac agagcttcac tcagcagcgc
     4021 gagcccacca acctgaccga tgccagagag gcccgccaat gcgccgcgga ccacacccag
     4081 cacggtggtg catttgggct cttcgtcgga gcaaccggcg tgggccagcg tcatccacgg
     4141 tcctgccacg gggatgcgca acttgtcggc ttcatcggcc ttgggccacg catacgattg
     4201 tgcgagggcc gcaccatacc agagcgcgaa cgtgccaacg cccgccagca ggaccagcgg
     4261 gcgcgccgat ggcgggggca aggcatccgg ttcagctgct gccgcactgc tggcggagct
     4321 actgctgggc ggcagcgcca tcggatcgga acctgcggag gcaggcgccg ggataggtgt
     4381 ctgcgttggg gcagcgccca agctgggagc ggttgcaggc cgagcgcctg cgcccgcggg
     4441 agggctcgcc tgaggatcat cggcccgcgc caccccctcc cagagcagga gtaaggcaca
     4501 cgcgaaggag gtagcgcgct tcacggggcg tgtttcttgc gcgagtgctc ctcgaacgca
     4561 agcggaatga gaaggcgtcg agtttcgcta gaggtcgcga cgacgttcga agctgttcta
     4621 agctacgggg tgccattccg ggacgacacc agcgtgcaag cgaatatttt tcgtcatctc
     4681 tccgaggact gccaggccct cgatatccag cgcggtccac aggcagcagt gcgtgcgacc
     4741 gcggcttcct tttcgccagg cgtgcgttgg tcagccccgt gttcgcaagc agtgctctcg
     4801 accgcctcgc gttcgcccgc agcactttca cgacgcgcgc tgctggcggg cacgttcgcc
     4861 ttcagcacgg gcgcattcca gcgttcggcc ttagcgcaag cagcgagtgt ttccgacgcg
     4921 ctgcgggcca gcttcgacaa actcgtcagc tgggcccaac gccaacgagt ggcgctcggt
     4981 gcggcggtgc tcgaggtgag caacggtcag ccgctcctcg cttcgggtga gctgctgccg
     5041 ctcaaccccg cgtccaacca aaagctcttg acgatggccg ccgcgctcga gcggctcgga
     5101 ccagcgcaac gtttctcgac ctcgctgcac gggcggctga cgggcgacac tctgagcgag
     5161 ctggtgctgc gcggtgatgg ggatcccgaa ctgacggtca ctcggctgga gcgaatggtg
     5221 cgttcgctcg ccgatcaagg cgtgcggcgc gtggccggcg atgtactcgt cgatcaaagc
     5281 gcgttcgatg cgcgctggga tccccccgcg tacgagcagc gtccgcgtga cgaggcggct
     5341 taccgcgctc cggtcagcgc cgtcgcactg gacggaaacc tcttggttct ccacgtcttc
     5401 ccgagtcgtg tgggggcgtc gggtgagcca tcacccgcca ggctttggct ggaaccaccc
     5461 gggctcgcgc agatcgaggg cagcgtcagc accgtctcga gcagccccgc cggcgtaagc
     5521 ttcagtgttc tctcgaaaga cggcaagctc gtcgcccgcg tgggtgggag cgtgctcgct
     5581 tcgggcggtg aagtgagctt ctctcggcgg ctgccggaac cggagctcgc ggccggacga
     5641 gtcctcctgc gcctactttc cgatcacggc atcgtcgtgt cagggcgact gcgttcgggc
     5701 ggcgccgaga tccaaagtga gctggtggcg ctgcgctcgc gctcactcgg tgagctactg
     5761 ctgccgctcg gcaagcaaag cgacaacttc gctgcagaaa tgctgttgaa ggcactgggc
     5821 gcgcgcagca ccgcgagcag cggcacctct gcggcgggag cagccgtgct catggattac
     5881 ttgcaaaaga ggggtttgct gctgccagga accacgatca cgaatggctc gggcttgttc
     5941 gatgccaacc gcttgacggc gctggccctc gcgcgcgtgc tacgcgaggc atacctggat
     6001 ccgcgttcgg gtccggagtt cctcgcggcg ctctcgatcg gcggaaccga tggcactttg
     6061 agccaccgct tcggggcgct caacgcgagt cggcgcgtcc gcgcgaagac gggaacgctt
     6121 tcagacgtga tcgcgctttc gggcttcgtg ttcgggtcca gcatggcgtc ggcccgctgc
     6181 ttctcgttcc tactgaacgg agtgtcgggg catcagactg aagccaagaa cctcgtcgat
     6241 cgagtcgtgc tagcgctcgc cgcggaagcc ccttgagtct gccgcgccac cgtcggcaag
     6301 cgagactact gaatccgtac ggctcacaaa gtagcaaagc ccgcggcgtt ggttgcgccg
     6361 cgggctttgt ccttcgcgcg tctatcgcta cttcgccgcg ctcggcggat ttacctgctg
     6421 gaagtaatcg atgtccttct gatactcccc gcggtcgttc acgctcagcg agaggaagag
     6481 aatca
//
LOCUS       DASTKU010000306        18549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1398987, whole genome shotgun sequence.
ACCESSION   DASTKU010000306 DASTKU010000000
VERSION     DASTKU010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 18549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18549
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398987"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VFQ61_10930"
     CDS             complement(<1..884)
                     /locus_tag="VFQ61_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HET9955011.1"
                     /translation="MEEQDRLTDVGQTIPHGLGSVYALPRLSLVVEREAGKPSERRLE
                     LDGDRIRVGSHPSNEVVLEDRLVSRFHCTLTLSRGAWAITDSGSLNGTFVSGVRIRDA
                     LLPTRECEIRVGASVLRIAPLDTANLVRVPIVSRFGELYGTSLAMRRVFDVLERVSQT
                     DATVLVEGESGTGKELVAHEIVRRGTRAQAPFLTIDCSSISPGLIESELFGHARGAFT
                     GADRVRIGAFEAAHGGTVFLDEIGEMPLEMQPKLLRALEAREIRRAGENDVKKVDVRV
                     IAATNRNLEREVNRGRFR"
     gene            complement(1035..1925)
                     /locus_tag="VFQ61_10935"
     CDS             complement(1035..1925)
                     /locus_tag="VFQ61_10935"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9955012.1"
                     /translation="MSLLPRRFGGMLLVLGCTISWLPMPARAEANGTDDMLFVPGGTF
                     AMGADAEGEQDERPRHSVEVSGFWLDRTEVTQSAYRTCVAAKACKPPASLRGSPLVNG
                     RASEFERGDHPVVGVSWFDANDYCRFRGRRLPSEAEWERAARGDDARRFAWGNEVPDP
                     TRHGVFGGRATTERVGSFPDGRGPYGHDDLAGNVWEWVADEYDPFAYRRASASTGQPG
                     TCAQILATLAELRATHQQGFTGKNPIPVECERVLRGGAYNYGSAGMRASNRVHHPARF
                     RVAVAGFRCAMDAPVTAGPG"
     gene            complement(1936..2640)
                     /locus_tag="VFQ61_10940"
     CDS             complement(1936..2640)
                     /locus_tag="VFQ61_10940"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET9955013.1"
                     /translation="MPTPNTAPAPRTQRGSPRMFESDFLERFSHVHPATPAVLYLPLA
                     AFSLYWAHFQNGIGFWRLALELFVGYLVWTLFEYWLHRLLFHMRVVGPKTARAYFFIH
                     GVHHDYPWDRTRLVIPPGASLFLCVVVYGMFRVILGGHDMFGPFAGFVVGYVLYDSVH
                     WYVHARAPKNAFGRWLRREHMVHHFKEPTSRFGVSCPWLDYVFGTRGRPVGARESDAS
                     ERNAQEQSPEHIPQAS"
     gene            2993..4798
                     /locus_tag="VFQ61_10945"
     CDS             2993..4798
                     /locus_tag="VFQ61_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955014.1"
                     /translation="MPEDVKPAVRSSPLQTAKAWLVRHGALLAYGVLALGLLAWVAWD
                     AFSVRLVTFGSTADYWEHSATLRALIESPFHPRNPHLVSAAPSPRFIPSFLLCALLAK
                     AMGGDALFAMGLAASLNTTLLCLAIYAFFRGYFRSRVAALYGLLFMFASARDAFHYSN
                     VYQLKVFFSVASYPSTTALALVLLGFAWEVRLLRVSVRRRWPELLGFSLYQAVILVTH
                     PLTAMMTFAGVPLLALTDRTHAGGWRALLRAIWKARPRMFAKTPTSTAPAAGCDGPIQ
                     AQPGENGPSLRARAEIALTTPCSLLLAQGWPYFSVWEVLRGGQHGDEPAWLEEALSTA
                     IKGDFGNSHLFYRWPDLSATLYLALLGVPVLLYLLARAQHLFLVLGAVAMLIPFFVNA
                     YVPLPLGHRFVLLAIFFLQAAVVWFLLALTPGTPQTFGILAEGSTAPRRVARWMALAL
                     VLGFLGYAVELNIDKARARFESTEQRYKGFDSPVVRYCRRIAELAGPDAVVLGPADLS
                     WPVPTFGPKVVSLLHENPLVPDAKRRIYDVKRFLDPRVQEEERLQIVQRYRVTHVLLK
                     GNAGGQLGEFLQAHARRTALPTGKSLFTLVSAGGH"
     gene            complement(4770..5777)
                     /locus_tag="VFQ61_10950"
     CDS             complement(4770..5777)
                     /locus_tag="VFQ61_10950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9955015.1"
                     /translation="MVPENAPSARHEIELSVVAPMYNEETNIEVFAERVSEVLESLQL
                     EFEIVLVDDGSRDGTWRSICESARRHPRLKGVKLSRNFGHQGALFAGLHHAEGRAIVT
                     MDGDLQHPPSAIPELLAAWRSGYKVVNTRRSDSADTSFFKRFTSRAFYWLFSGLSGVR
                     MEAGASDFRLLDRSALDALLSMGDADLFIRGLVNWLGFTTITLPYQAQNRHSGVPKFT
                     FKKMLRFSTGAMLSFSVVPLRLGIGIGLITSFFAFLELCYILFTYFTGHAIPGWASVM
                     TVMSLMFGVLFVLLGVIGAYLGKIYEILKNRPRFVIEALTTTTAIESDRRFSGRLRSP
                     A"
     gene            5915..7738
                     /locus_tag="VFQ61_10955"
     CDS             5915..7738
                     /locus_tag="VFQ61_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955016.1"
                     /translation="MRSHLLAWPTALRDRLLAWPSTLKDHLRSLSVTELRRKIRDTSP
                     LGWLYFASCVLVIGVFLAYFSSWWSGYAPALLDTNLQTDDARTALFPFHRYAPGAPLA
                     NDPIANEMIEYQPYAFRLIYRIGVPLKGLLWAAKLAQFVCLSIVAYAAWVMLRSRRAG
                     LGPALLFLFAFLHDNFVMNRIGGGLPRSFGFPAMSVWLAGVLAHNLRARRAGAIIAAF
                     TYPSALAMILAAEGMYTLRGLARPGWRTTWRRLRHYLLLVAACAALFAPTALLGASDG
                     GPVHTLAQAEQEPAFSKAGRLWLLPLPNAGAVFGEHLTQTFEPLGESPVSSLRDALDP
                     RHHEVALVMVGITLLLPLLGMAQAPLGVLAFAGSAFLMYALSIVFAFRLYSPERYYSF
                     GMHVVGLGLFVNALGLTLPRLQPRIRRLVRNCVVALTIFLVWGGLGDGLAPRGRMAMT
                     IDYRRRAPLWEFIRTLPKDVRIASFISDGDDIPLFAQRANNGGFETLQPWLTLSWARQ
                     KARAEDTLRAFYATDHETVLSYARKYRVSHLLVNRSRYRGDIIRQSRSFEPLTTFARE
                     LLERADLNGLVLANIPAPAIIYSHGNLDLVDVEKLQRAWDN"
     gene            7775..8752
                     /locus_tag="VFQ61_10960"
     CDS             7775..8752
                     /locus_tag="VFQ61_10960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9955017.1"
                     /translation="MKRRLFPFFLAGAVALTGSAGTHYARATVVERIVAVVGERAILL
                     SDLRTRARPILLQVHQSVPEGAQRNAAISQVYKTVLDRLIDEELQFRAAQQAKLTVST
                     KEIDEALSRLATQNKLALPDLLAEAERSGLTEASYRDELRRQLLEAKLVNTRLQGRIR
                     VVDEDLRQAYQKLAMEERQRLSMTPAWILIPAGKDSAEQRRQRELAERVSREAQTQDF
                     AKLAAQYSDDKASRATGGRLASTRPAELPPALARVALGLEVGQVSAPVRYGDHYAVLK
                     VLARDASELPDFEQARQELTERVYIDKMTQAKKTWLDGLRRQHHVEIRL"
     gene            complement(8749..10224)
                     /locus_tag="VFQ61_10965"
     CDS             complement(8749..10224)
                     /locus_tag="VFQ61_10965"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9955018.1"
                     /translation="MFAASVGIGVYAVRRGGSVASAPGVLTASGSAPVLGSAAPRSPA
                     IQEEHDAPPVMPQPLAEVAGRTRQEVVTPAAWPSFPLGRLAGPAAGGALLPVGAQSMA
                     VGTDQAVATLTLFGDLQCPYTRAALRTALRQSSRFGSALRVSFHHRPLVEHPLAANAA
                     AILAAVAERRGGAAAFAVLAAAAQSDAEPSLDEIDRWLRAAGVPESANALLASGQGKA
                     RLLADLHLAAELSVLRTPTWFVNGRRLSGDRGDEDIERLVLQEGPAVRWLRAQGHVDD
                     RAYALRVQKNLIGVEGEASDRSCVPTDAAPQIGPRDAPLTLVEFADFESGPCQELAQN
                     LALVLPRYADSVHRVFYALPKAREPRSQLAAASGLAIRALSGDAGFWQVHAVLRSAEG
                     RLDTRLHRAAAQLGLSDEQIRSAAAQPVRDFELKRAAGLAESLAIAYVPTFFLNGRRF
                     EGLRSVAELQRIFTEELATARRLERAGTPPAAFGRLLCGEL"
     gene            10492..11595
                     /locus_tag="VFQ61_10970"
     CDS             10492..11595
                     /locus_tag="VFQ61_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955019.1"
                     /translation="MTPSSSPSHSQAIGRAWLRLLWRLTLAACVISGVGAFASEAAAQ
                     SIAITNTNTIVRRSNRPSGLLTTTINQADCAANDTLGIKMNVVVGGLSSPKVSVVAST
                     MAACDTDTAALADANRCWPVDETTSFTETFELRVQKIIKPRADGGDVCDSTDDSIIGV
                     SLYFLLMDGATVNKSTKLNVKYDLAGPAPPTGLSIGSGENKLYPKWPLKTNSFELIKQ
                     YNVYCELASPDSVVTGAGDGAAGAGTSGGCTSENLVPGKPAPDDLRKGFSAPVAGKGV
                     AKGLENYSVYACGIAVVDKNENVGVLSELVCGMPEPVTGFYDAYRGAGGEAGGGYCAF
                     GRQPAAAGAGLIALAGAGLVARRRKRVGRGQGA"
     gene            11595..12377
                     /locus_tag="VFQ61_10975"
     CDS             11595..12377
                     /locus_tag="VFQ61_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_2562 family outer membrane beta-barrel
                     protein"
                     /protein_id="HET9955020.1"
                     /translation="MIRKLGRRPCSCLGLGTCSALLAGAVLLTGTPAAAQDTTLGFAR
                     HKPYVESPQSVAFELRFGRYIPDVDDGLGEAPYNTIFGSKNRFYGGFEIDWQALRIPY
                     LGTLGAGYGLGYTISSAKAISVTGDRSDEDTTLQILPMYLVAVLRADVVAKHTVIPVV
                     PYAKLGLGYALWRTEDGGDTPRVDGVIGRGRSYGYHAALGGMFMLDWFDVADARTADA
                     NIGLNHSYVFAEWFVSKLDGFGSKDQLNVGTSTWTAGLAFEF"
     gene            complement(12639..13412)
                     /locus_tag="VFQ61_10980"
     CDS             complement(12639..13412)
                     /locus_tag="VFQ61_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955021.1"
                     /translation="MATAVLRVPDPRVVPLPLPFVTPAGPQIFVHEGARQALERRFMA
                     AFGGPVQLAVTDNRRRMVTHSCTKGTLQVRVHMMFLGAADRVRDALVAYVVRGDKEAS
                     QIVGEYIDQNLHRIRASRPVPGPLKTRGEVHDLVPILAEINAEYFGGALSDVLITWGR
                     RSATRGGTRHSIKLGSYSAVERLVRINPVLDKPWVPRYFVAYIVYHELLHHVVPEVRS
                     GGRTILHPPEFQRREREFRHFERAVAWEHKHIDRLLRSR"
     gene            complement(14096..15310)
                     /gene="recA"
                     /locus_tag="VFQ61_10985"
     CDS             complement(14096..15310)
                     /gene="recA"
                     /locus_tag="VFQ61_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET9955022.1"
                     /translation="MSVLDEKQKTKALELAIASVQKEFGDGAIMRLKDGEKIGGEVQV
                     VPTGSLGLDVALGIGGYPRGRIIEIYGPESSGKTTLTLHAIASVQRRGGVAAFIDAEH
                     ALDVTYAKKLGVKTEELLISQPDYGEQALEIADMLVRSGAVDLVVVDSVAALVPKAEI
                     EGDMGDSHVGLQARLMSQALRKVTGSVSRSQCLIFFTNQIRMKIGVMFGSPETTTGGN
                     ALKFYASVRLDVRRVGAIKEAGSEGKDPVVIGNRTRVKVVKNKLAAPFREVEFDILYG
                     QGVSRSGEVLDMSVDANLVQKSGAWFAADGERIGQGRDNARQYLEQHPELLERLEQRV
                     LQQHGILRGAAGDPKAEAGRTEKDAKSDPKDSKADSKGKGEAGEGKAAPDAKAPPNGA
                     SSAARRPMPRPS"
     gene            complement(15632..16189)
                     /locus_tag="VFQ61_10990"
     CDS             complement(15632..16189)
                     /locus_tag="VFQ61_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955023.1"
                     /translation="MKMLSNFHVVASLCLVAAVGLGLGQGCTTDDSDDTSTGGKASGG
                     KTGATGGAGGQAGEGTTEGGTTSAEGGQTSQAGETSTGGAATGSGGASAGGETANGGA
                     AGAPDTCQSPAEGQSDAKGMSCADYCAAFFESCADYNDANETFENEASCLTYCSNYSD
                     EQLCCRAHYVMAPTCAKAAGDSECN"
     gene            complement(16281..17501)
                     /locus_tag="VFQ61_10995"
     CDS             complement(16281..17501)
                     /locus_tag="VFQ61_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET9955024.1"
                     /translation="MLTLALTILWSLMVSFACSIAESVVLSISHAQIQALGKSKAAKI
                     LRHFKREVDIPIAAIVAFHTVAHTVGASVSGAIYVHVFSEQTLWVFSLVFTVLVLIFS
                     EIVPKTLGVTYVAQFAVPVAYTVSALVVVLKPVIWVTNLFAKLLRRGQEAPVTSIDEI
                     RLLVAFGRNEGALGGRLADMIEGAARLRELTAYDVMVPRAQVIALSGDRGLEENLAVI
                     RRSGHSRFPFSAEGDIDKVEGVVLAKELFFALRESGDDVDFRKLLSPLLVVPTSAPLE
                     RVLRTFQEERRHMAIVVDEYGGTQGIVTLEDVLEEIVGEIEDESDRVDPSIVRRAPGV
                     FSCRGSAETRKVFDLLNIESESEMVTLAGFVAELVGRVPRVGDNVEWEGHRFSVTRAS
                     ARRAERIEVRRLQS"
     gene            complement(17536..>18549)
                     /locus_tag="VFQ61_11000"
     CDS             complement(17536..>18549)
                     /locus_tag="VFQ61_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955025.1"
                     /translation="PLSKPEDELIEAMKKGPVALDVDRMWQLLGFNGPAVQVQDADGR
                     PLAIGLEDLLGGLEKHWQEDASDLNRGRLFAQELMKYARFEKAEKTLAKIVALGGTGE
                     DWLALGVTQLQQEKWDKAEGTLRGAQNLLPDNPFPLLHLAKAYQGKGNPTEERAMVEK
                     AIELAPNSVDAWAYLFSQIQRNESEEAAERAVTQLANNEANRKTAAPFIAVQGIYAAN
                     EETRDKALDWAKKAVERNDNDPLALICLSALYGQRGELDRVIEVLSRHESKMANDVRL
                     AHNYFEALFQKRQLEKVTKLLNALAGSQNREVKQFAVERSRMVAQYLQQQQQQLAGSQ
                     RKA"
BASE COUNT         3430 a         6033 c         5636 g         3450 t
ORIGIN      
        1 tcgcgaaagc ggccacgatt gacctcgcgc tccagattgc ggttcgtggc cgctatgacg
       61 cgcacgtcca ccttcttgac gtcattctcg ccggcgcgtc gaatctcccg cgcctccaga
      121 gcgcgcagta gcttcggctg catctcgagc ggcatctcgc cgatctcgtc caaaaagacg
      181 gtgccaccgt gggcagcctc gaacgctccg atccgaactc ggtctgcacc ggtgaaagca
      241 ccgcgcgcgt gcccgaatag ctcgctctcg atcagcccgg gagagatcga actgcaatcg
      301 atcgtcaaaa atggggcttg cgcgcgtgta ccccgacgca cgatctcgtg agcgaccagc
      361 tctttgccgg taccgctctc accctcgacg agcacggttg catccgtttg agagactcgc
      421 tccagcacgt cgaacactcg acgcatcgcc agtgacgtgc cgtacagctc cccgaaacgc
      481 gagacgatcg gcacgcgcac caggtttgcg gtatcgagcg gcgcgattcg gaggacgctc
      541 gcgcccaccc gaatctcgca ctcgcgagtg ggcagcaacg catcccgaat ccgcacgccc
      601 gagacgaacg tgccattcaa ggaaccggag tccgtgatgg cccaggcgcc gcggctcagc
      661 gtgagggtgc aatggaagcg ggacaccaac cgatcctcca gcaccacctc gttgcttgga
      721 tgggagccca cgcgaatgcg gtccccatcg agttcgaggc gacgttcgct gggcttaccc
      781 gcttcccgtt cgaccaccag cgagagcctg ggcagcgcgt acaccgaacc caaaccatgc
      841 ggtatcgtct gacccacgtc ggtaaggcgg tcttgctctt ccatcggtcc tcatcctgct
      901 ctgcgaacgg ccaaaattat agcggaaaag gcaagcggcc gtgttctacc cattcggcga
      961 cccagtttcg cccgccgggc agcacgcgcg ggctgcgctt cgacggccgc tccgcttcct
     1021 gagacgagtg cgactcagcc cggcccggcc gtcacgggcg cgtccatggc acagcgaaaa
     1081 cccgcgactg caacgcgaaa ccgagcaggg tggtgcaccc ggttcgaggc tcgcatgccc
     1141 gcacttccgt aattgtatgc gccccccctc agcacgcgct cgcactcgac ggggatgggg
     1201 tttttgccgg taaagccctg ttgatgcgtg gcccgtaact ccgcgagcgt cgccagtatc
     1261 tgcgcgcacg tccccggctg gcccgtactc gcgctggcgc gccgatacgc aaacggatcg
     1321 tactcgtcgg cgacccactc ccaaacattg ccggccaaat cgtcatgacc atagggcccc
     1381 cgtccgtcgg ggaacgaacc cacccgctca gtggtggcgc ggcccccgaa aacgccgtgt
     1441 cgcgtgggat cggggacttc attaccccag gcaaatcggc gtgcgtcgtc gccgcgcgcc
     1501 gcgcgctccc actcggcctc gctcggtaga cgtcgacccc tgaagcggca gtagtcgttg
     1561 gcgtcgaacc aggacacccc gacgacgggg tggtctccgc gctcgaactc cgacgcacga
     1621 ccgttcacga gcgggcttcc gcgcaagctc gccggaggct tacacgcttt cgcagccacg
     1681 cacgtgcgat atgcggactg cgtcacctcc gtgcgatcga gccagaatcc cgaaacttcg
     1741 accgagtgac gaggtcgctc gtcctgctca ccttcagcgt cggctcccat cgcgaaggtt
     1801 ccccccggga cgaacaacat atcgtccgtc ccgtttgctt ccgctcgcgc cggcatgggc
     1861 aaccaactga tggtgcaacc gagcaccagt agcatcccgc cgaatcgcct gggcagcagg
     1921 ctcatgctct cgaactcagc tggcctgcgg gatgtgctcc gggctctgct cctgcgcatt
     1981 gcgctcgctc gcatcggatt cgcgagcgcc caccggacgc ccgcgcgttc cgaacacgta
     2041 atctagccag gggcagctca cgccaaagcg gctcgtgggc tccttgaaat gatgcaccat
     2101 gtgctcccga cgcagccagc ggccgaacgc attcttgggc gcgcgcgcat gcacgtacca
     2161 atggacgctg tcgtagagca cataaccaac cacgaagccg gcgaacggcc cgaacatgtc
     2221 gtgcccgccc aggatgaccc ggaacatgcc gtaaacgacg acgcacagga agagcgacgc
     2281 tcccggcgga atgacgagcc gcgtgcggtc ccagggataa tcgtggtgca cgccgtgaat
     2341 gaagaagtag gcacgcgccg tcttgggccc caccactcgc atatggaaca agaggcggtg
     2401 caaccagtac tcgaacagcg tccaaaccag ataaccgacg aagagctcca gcgcgagacg
     2461 ccagaaacca atcccgttct gaaagtgcgc ccaatagagc gaaaacgccg ccaaaggaag
     2521 atagagcacg gccggagtgg ccgggtgcac gtgcgaaaag cgttcaagga agtcggactc
     2581 gaacatgcgg ggtgagccgc gctgggtccg cggggcgggc gccgtattgg gtgttggcat
     2641 cacagtctct tcgacggtcg ttgtgaacgg gccttttctg aaccgtttct ttggcgttct
     2701 gtagcggcta cacggggaac gtctactgtt tcgtccacgt cactcgatct ttcgcaacgt
     2761 ggagcgacaa tgccgcttcg ttctctcgtg cgtgcgaagg cattgcagga gcacgctctc
     2821 agccaaacct cgctcgtttc tggcccgtct caagctcaag cggcgaataa cgtgctccca
     2881 cagcccatcg gcggcaaggg caaaccacga tttcaggcgc acgaatcggc cacccgggca
     2941 aagaacgtcg cttgatcgcg ccgcgtgcac atgacaacca gctgcgcgac cgatgccgga
     3001 ggacgtgaaa cccgcggtgc gcagtagccc ccttcaaacc gcaaaggctt ggcttgttcg
     3061 ccatggcgcg ctcctcgctt acggggtcct cgcgttgggg ttgctagcct gggtcgcttg
     3121 ggatgccttt tccgtgcggc tcgtgacgtt cggatcgacc gcggactact gggagcactc
     3181 cgccacttta cgggcgctga tcgagagccc gttccaccct cggaatcccc acctcgtcag
     3241 cgcggcgcca tcgccacgct tcattccctc gttcttgctt tgcgcactcc tcgccaaagc
     3301 catgggtggt gatgcgctgt tcgcgatggg gctcgcagcc agcttgaaca cgacgctgct
     3361 ctgcttggcc atttacgcgt tctttcgcgg ctactttcgc agccgagtgg cggcacttta
     3421 cgggctgctc ttcatgttcg cgagcgcgcg cgacgccttt cactattcga acgtctacca
     3481 gctcaaggtc ttcttcagcg tcgccagcta tccctccacc acggccttgg ctttggttct
     3541 gctcggcttc gcgtgggaag tgcgcttgct tcgagtttcc gtgcggcggc gctggcccga
     3601 gctgctcggt ttctcgctgt atcaagcggt cattctggtc actcacccac tgaccgcgat
     3661 gatgaccttc gccggtgtgc cgctactggc gctgaccgat cggacccacg cggggggctg
     3721 gcgcgcgttg cttcgggcca tttggaaggc tcggcctcgc atgtttgcaa agacgcccac
     3781 cagcacggct cctgccgcgg gctgtgatgg gccgattcag gcgcagcccg gcgagaacgg
     3841 gccgagcttg cgagcgcgcg ccgaaatcgc gctgaccacc ccctgctctt tgctactggc
     3901 tcagggctgg ccttactttt cggtttggga ggtgctgcgc ggcggccagc acggagacga
     3961 gccggcctgg ctggaggagg ccttgtcgac cgcgatcaaa ggcgatttcg gcaactccca
     4021 tctcttctat cgctggcccg acttgtccgc gacgctctat ctcgcgctgc tcggggtgcc
     4081 tgtcttgctg tacttgctgg ctcgggcgca gcacttgttt ttggtgctgg gcgcggtcgc
     4141 gatgctgatt ccgttcttcg tgaacgctta cgttccgcta cccctggggc atcgtttcgt
     4201 cctgctcgcg atcttcttcc tacaagccgc ggtcgtgtgg ttcttgctcg cgctcacgcc
     4261 ggggacgccg cagacgttcg gaatactggc ggagggctcg accgcgccgc gccgcgtggc
     4321 ccgatggatg gcgctcgcct tggtgctcgg cttcctcggt tacgcggtgg agttgaacat
     4381 cgacaaagcg cgagcgcgtt tcgagagcac cgagcagcgc tacaaaggct tcgacagccc
     4441 ggtagtgcgc tattgccgcc gaattgcaga gctggctggc cccgatgcgg tggtgctcgg
     4501 tcccgccgac ttgagctggc ccgtacccac ctttggcccc aaagtcgtat ccttattgca
     4561 cgagaacccg ctcgtgcccg acgcgaaacg gcggatttat gacgttaaac gcttcttgga
     4621 ccctcgcgtc caggaagaag aacgcctgca aatcgtgcag cgttaccgcg tgacccacgt
     4681 tctgctgaag ggcaacgctg ggggtcagtt gggcgaattc ctgcaagcgc atgcacgtcg
     4741 aacggcactc cccacgggca agtcgctgtt cacgctggtg agcgcaggcg gccactgaaa
     4801 cggcggtcgc tctcgatcgc cgtcgtggtc gtgagcgctt cgatcacgaa gcgcggccgg
     4861 ttcttcagga tctcgtagat cttgcccaag taggccccga tcacgcccag taacacgaag
     4921 agcacgccaa acatcagaga catcaccgtc atcaccgagg cccagcccgg aatggcgtgc
     4981 cccgtgaagt acgtgaacag aatgtaacag agctcgagaa acgcgaagaa gctcgtgatc
     5041 agaccgattc cgatgcccag ccgcagcggg acgacggaga aggaaagcat ggcccccgtc
     5101 gagaagcgca gcatcttctt gaacgtgaat ttcggcactc ccgagtgccg attctgggcc
     5161 tgatacggca gggtgatcgt agtgaagccc aaccagttga cgaggccgcg aatgaacaga
     5221 tccgcgtctc ccatgctgag cagagcgtcc aacgccgagc gatcgagcag ccggaaatcc
     5281 gaagccccgg cctccattct gacgcccgat aggccagaaa aaagccaata gaacgcgcgc
     5341 gaggtgaagc gcttgaagaa gctcgtatca gcgctgtcgg agcgccgtgt gttcacgacc
     5401 ttgtaaccgc tccgccaagc cgcgagcagc tccgggatgg cgctcggcgg gtgttgcagg
     5461 tccccgtcca tggtgacgat tgctcgccct tcggcgtggt gcaagcccgc gaagagagcc
     5521 ccctgatggc cgaagttgcg agacagcttg acgcccttga ggcgggggtg gcggcgagca
     5581 ctttcacaga tggaccgcca ggtcccgtcg cggctgccgt catccacgag cacgatttcg
     5641 aactcgagct gtagactctc cagcacctcc gaaacccgct ccgcgaacac ctcgatgttg
     5701 gtctcctcgt tgtacatggg agccaccacg gaaagctcga tctcgtgtcg ggcactcggg
     5761 gcgttttctg gaaccatggg ccgccgatac tactcgcggg tcttggtcgc aaagtgaatc
     5821 gtgaacccga acccccctgg ccattttcag cgttctgcac gccgccgtct cacttttatt
     5881 gcgggcttcg gcgtgctcgg ctagcacgct cgccgtgcga agccatctcc tcgcctggcc
     5941 gacagcgctc cgtgatcggc tgctcgcctg gccgagcacc ctgaaagatc atttacggtc
     6001 gcttagcgtc accgagctgc gacgcaagat ccgcgacacg agcccgctcg gatggcttta
     6061 cttcgcgagc tgtgtgctcg tgatcggcgt gttcctggcc tacttctcga gctggtggag
     6121 cggctacgcg cccgcgttgc tggataccaa cttgcagacg gacgacgcta gaacggcgct
     6181 gttccccttt catcgctatg cacccggcgc gccgctcgcg aatgatccga tcgccaacga
     6241 gatgatcgaa tatcagccgt atgcatttcg gctgatctat cggatcggag tgccactgaa
     6301 gggcctgctc tgggccgcga aattggccca gttcgtgtgc ctgtccatcg tggcatacgc
     6361 tgcctgggtc atgctcagga gtcgacgcgc gggcctgggc ccggcgcttc tgttcctatt
     6421 cgcattcttg cacgacaact tcgtgatgaa tcggatcggc ggaggcctgc cgcgcagctt
     6481 cggttttcca gccatgtcgg tctggctcgc cggtgtgctc gcccataacc tgagagcccg
     6541 ccgcgcaggt gccatcatag ctgcgtttac ctatccttcc gcgctcgcca tgatcctcgc
     6601 cgcggagggc atgtacacgc tgcgcgggct tgcaagaccg ggctggcgca cgacttggcg
     6661 caggctgagg cactatttgc tactcgtggc cgcgtgcgcg gctctttttg cgccaacggc
     6721 gctgttggga gcgtccgacg gcggccccgt acacacgctg gcccaggccg aacaggaacc
     6781 agcattcagc aaagcgggca gattgtggtt gcttccgtta cccaacgcgg gcgccgtatt
     6841 tggagagcac ctgacgcaaa ctttcgagcc gttgggtgag tccccggtga gcagcctacg
     6901 cgacgcgctg gatccacgtc atcacgaagt cgccttggtg atggtcggga tcacgttgct
     6961 gctcccgttg ctcggcatgg cccaagcgcc gctcggcgta ttggcgttcg ccggctccgc
     7021 gttcctgatg tacgcgctct cgatcgtgtt cgcgttccgt ctctattcac ccgagcgtta
     7081 ctacagcttc ggaatgcacg tggtggggct agggctgttc gtaaatgcgc tcgggctcac
     7141 gttgccccgg ctgcaaccac gcatccggcg cctggtgcgg aactgcgtcg tcgcgctgac
     7201 catttttttg gtttggggag ggctcggtga cgggctggcg ccgcgcggcc gcatggctat
     7261 gaccatcgac tatcgacggc gcgccccgct ctgggagttc attcgaacct tgccgaagga
     7321 cgtccgcatc gcgtcgttca tcagcgatgg agacgatatt cccttgttcg cgcagcgcgc
     7381 caacaatggc ggcttcgaga ccctacaacc ctggctgacg ctctcgtggg cgaggcagaa
     7441 ggcgcgcgcc gaggacacgc tgcgcgcgtt ctatgcgacg gatcacgaga cggtcctgag
     7501 ctatgcgcgc aagtatcggg tgagccattt gctcgtgaac cgctcgcgct atcgagggga
     7561 catcatccga cagtcgcgct cgttcgagcc gctcaccact tttgcgcgtg agcttctgga
     7621 acgggccgac ctgaacgggc tggtgctggc caatatcccg gccccagcca tcatttattc
     7681 gcatggaaac ttggacctgg tggacgtcga gaagctccaa cgcgcttggg ataactgacg
     7741 gccagcacgg ttcgtgctac ctgggaccct catcgtgaag cgccgcctgt ttccgttctt
     7801 cctcgctggg gctgtggccc tgaccggctc cgccggaacc cactacgcgc gagccactgt
     7861 cgtggagcgc atcgtcgccg tagtgggaga acgcgccatc ctcttgagcg atctcaggac
     7921 acgcgctcgc ccgatcttgc ttcaggtgca ccagagcgtg cccgagggcg cacagcgcaa
     7981 cgcggcgatt tcgcaggtgt acaagacggt gttggatcgc ctgatcgacg aagagctcca
     8041 atttcgggcc gcgcagcagg caaagctgac cgtcagcacc aaagagattg acgaggcttt
     8101 gtcgcgtctc gcgacgcaga acaagctcgc gctgcccgat ttgttggccg aagccgagcg
     8161 ctcgggactc accgaggctt cctatcggga cgagctgcgg cgccagctgc tcgaagccaa
     8221 gctggtcaac acgcgcttgc agggtcgcat ccgcgtagtg gatgaggacc ttcgccaggc
     8281 ttatcagaag ctcgcgatgg aggagcggca acgcctgtcc atgacgcccg catggatcct
     8341 gattccggca ggtaaagact cggctgagca gcgccggcag cgtgaactcg cggagcgtgt
     8401 gtcccgcgaa gcgcaaacgc aagacttcgc gaagctcgcg gcgcaatact cggatgacaa
     8461 ggcttcgcgc gccaccggtg ggcgcttggc gagtacgcgc ccggctgaac ttccaccggc
     8521 tctcgcgcgc gtcgcgctgg gtctggaggt ggggcaggtc tccgcacccg tgcgctacgg
     8581 cgatcactac gcagtgctca aggtgttggc gcgcgatgcg agcgagctgc cggacttcga
     8641 acaggcgcgc caggagctca ccgagcgcgt gtacatcgac aagatgactc aggcgaagaa
     8701 gacctggttg gacggcttgc gccgccaaca tcacgtcgaa attcggctct agagctcccc
     8761 gcacaataga cgtccgaacg ccgccggcgg cgtgccggct cgctccaagc gacgcgcagt
     8821 agccaattct tcggtaaaga tgcgctgtag ctccgcgacc gagcgcaacc cctcgaagcg
     8881 acgaccattc aagaagaagg tcggtacgta cgcgatcgcc aagctttcgg ctaaacccgc
     8941 ggcgcgtttg agctcgaagt cgcggaccgg ttgcgccgcc gcacttcgaa tttgctcgtc
     9001 gctcaagccg agctgcgcgg cggctcgatg caaacgcgta tccaagcgcc cctccgcaga
     9061 acgcaacacc gcgtgcacct gccaaaaccc ggcatcgccg gacaacgcgc gaatggccag
     9121 ccccgacgcg gcggcgagct gggatctggg ctctcgcgcc ttgggtaagg cgtagaagac
     9181 tcgatgtacc gagtccgcgt atcgcggcaa caccagagcc agattctggg ccaactcctg
     9241 gcatggtccg gactcgaaat ccgcaaactc gaccagggtc aacggagcgt cacggggccc
     9301 gatttgtggg gcggcgtccg tcggcacgca gctccgatcc gaagcttcgc cttccacccc
     9361 gatcaggttt ttttggaccc gcaacgcgta ggcccgatca tccacatgcc cttgcgcacg
     9421 cagccaacgc acggcgggcc cctcttgcaa gacgagccgc tcgatgtcct cgtcaccacg
     9481 atcgccggag agccgacgtc cattcacgaa ccacgtcggg gttcgcagca cggacaactc
     9541 tgcggccaag tgtagatccg cgagcaagcg agccttaccc tgaccactcg cgagcagcgc
     9601 gttggcggac tcgggcactc cggccgcccg cagccagcga tcgatctcat ctagactcgg
     9661 ctcggcatcc gactgtgcgg ccgcggcgag caccgcaaaa gccgcggctc ccccacgccg
     9721 ctcggccact gcggccagaa tcgctgccgc attggccgcg agagggtgtt ccaccagagg
     9781 gcgatgatgg aagctcacac gcaaagcgga cccgaagcgc gaactctggc gcaacgccgt
     9841 cctcagcgcg gctcgcgtgt aagggcactg cagatccccg aaaagagtca gcgtagccac
     9901 tgcctgatcg gtgcctaccg ccattgactg ggccccgacc ggcaacagcg ctccgcccgc
     9961 ggcgggccca gccaagcgcc ctaatgggaa actcggccaa gccgcagggg tgaccacttc
    10021 ctggcgcgtc cgcccggcaa cttcggccag cggctgcggc atcactggag gcgcatcgtg
    10081 ctcttcctga atcgcgggcg agcgaggcgc cgcgctcccg agcactggtg ccgaaccaga
    10141 agccgtcaac acacccggag ccgaggccac cgagccaccc cgtcgcaccg cgtagacccc
    10201 gatccccacc gacgcagcga acatagcggg agcagcgatc cagatcagac ctagcgccca
    10261 ccgctccgcc caagactttc gaaccggggg cgaagcatcg gcgggcattc cccttgaatc
    10321 taacaggcga ctgacacttt ggcagtccgg acctggggct ccctcgacgg gatgccagaa
    10381 agtccagcga gtgcagccga acatcccatt cgcacgtgcc cagcccagcg aattgcccga
    10441 gcaatacatc cgttgcacgc tggcacggcc tgtgctctta aggaccaagc cttgacaccc
    10501 tcatcttctc cgtcgcactc ccaagcgata ggccgcgcgt ggctgcgtct gctctggcgc
    10561 ttgaccctcg ccgcctgcgt aatcagcggc gtcggcgcat tcgcaagcga agccgcggcg
    10621 cagagcatcg cgatcacgaa tacgaatacg atcgttcgcc gctccaatcg cccgtcgggc
    10681 ctgttgacga ccacaatcaa tcaggccgat tgcgctgcga atgacacgct gggcatcaaa
    10741 atgaacgtcg tcgtcggcgg gctgtcgagc ccgaaggtat ccgtcgtcgc ctcgacaatg
    10801 gcagcttgtg acacagatac cgcggcactg gcggatgcga accgttgctg gcccgttgac
    10861 gaaacgactt cattcaccga gacgttcgag cttcgagtcc agaaaatcat caagccccgt
    10921 gccgatggcg gggacgtttg cgactcgacg gacgactcga ttatcggcgt gagcttgtat
    10981 tttctactta tggacggcgc gacagtgaac aagtcgacca agctaaacgt caagtatgac
    11041 ctcgctggtc cagccccgcc gaccggactc agcatcggca gtggcgaaaa taagctctat
    11101 cccaagtggc cgctcaagac gaacagcttc gagctaatca agcaatacaa cgtttactgc
    11161 gagctcgcgt cacctgactc cgtcgtgacg ggtgcaggtg atggtgcggc cggtgccgga
    11221 acgagcggag gctgcacctc agagaacctg gtgcccggaa agcccgcgcc agatgatctg
    11281 agaaagggct tcagcgcacc cgtggctggc aagggagtag ccaaaggctt ggagaactac
    11341 tcggtctacg cgtgcggcat cgctgtggtc gataagaacg aaaacgtcgg cgtgctctcg
    11401 gagttggtgt gcggcatgcc tgaaccagtc acgggatttt acgacgctta ccgaggtgcc
    11461 ggcggggaag ccggcggcgg ctactgcgcc ttcgggagac agcctgcggc agctggagcg
    11521 gggctgatcg cgttagctgg ggcggggctc gttgcgcgcc gccggaagcg tgttggacga
    11581 ggtcagggcg cgtgatgatc cgaaaattgg gccgaagacc atgttcatgt ctcgggctcg
    11641 gcacctgttc agcgctcctc gccggtgccg tcctgctcac cggcacgcca gccgctgccc
    11701 aagatacgac actcgggttt gcacgacata agccttacgt cgagtctcct cagtcggtgg
    11761 ctttcgagct ccgttttgga cgctatattc ccgatgtgga tgatggcctt ggggaggcgc
    11821 cttacaacac catctttggc tcgaagaacc gcttctacgg gggattcgag atcgattggc
    11881 aggcgctgcg cattccgtac ctaggtacgc tgggcgcagg ttatgggctc ggctacacga
    11941 tatcttccgc caaagccata tccgtcacgg gagatcgcag cgatgaagac accacgctgc
    12001 aaatcctgcc gatgtatctg gtcgcagtgt tgagggcgga cgttgtggcg aagcacacgg
    12061 tgatcccggt cgtcccgtac gcaaagctag gcctcggcta cgcgctgtgg cgcacagaag
    12121 acggcggaga tacaccgcgc gtcgacggcg tcatcggccg gggtcgctcc tacggttatc
    12181 atgcggcgct cggcggaatg ttcatgctgg actggttcga cgtcgcagat gcccgtaccg
    12241 ccgacgcgaa tattggcctg aatcactcgt acgtcttcgc ggagtggttc gtcagcaagc
    12301 tcgatgggtt cggctcaaaa gaccaactga acgttgggac gagtacgtgg acggctgggc
    12361 tcgcgttcga gttttgagct ggagtcgctc aagttaagaa cggctcctga gacatgagtt
    12421 ctcggaggtg gccttggtca cctccgggaa cggttgctcg agccgcgcag aataacgact
    12481 cagcgtcgca ccggactcgc gtgcacgagc gcgaccggca ggtcttggct gcgcccggtc
    12541 gagtgcggct tagcccccac cccgcggagt gatctagatc gcccttcgct gggcagagcg
    12601 gaggctggcc tagaggtgct agccgggtcc ggatgccgtc agcgcgaccg caaaagacgg
    12661 tcgatgtgct tgtgctccca cgcgacggcc cgttcgaaat gccgaaactc acgctcgcgg
    12721 cgctggaact cgggcgggtg caggatcgtg cgccccccgc ttcggacttc cggaacgacg
    12781 tggtgcagga gctcgtggta cacgatgtag gcgacgaagt agcggggaac ccagggctta
    12841 tcgagcaccg ggttgatccg cactagtcgt tcgacggcgc tatagctgcc cagcttgatg
    12901 ctgtgtcgag ttccaccccg ggtagccgag cgccgacccc aggtgatcag cacatccgag
    12961 agcgccccac cgaagtactc cgcgttgatc tccgcgagaa tgggcacgag gtcgtgcacc
    13021 tctccccggg tcttgagcgg ccccggcaca ggccgagaag cgcggatccg gtgcagattc
    13081 tggtcgatgt actcgccgac gatttgagag gcttctttat cgccgcgcac cacgtacgcg
    13141 accaaggcgt cccgcacgcg atccgcagcg cccagaaaca tcatatgaac ccgaacctgc
    13201 aaggtcccct tcgtgcagct gtgggtgacc atgcgacgcc ggttgtccgt cacggcaagc
    13261 tgaacggggc caccgaacgc agccatgaag cgacgttcca acgcttggcg tgcaccttcg
    13321 tgcacgaaga tttggggacc ggcgggtgtt acgaaaggca gcggcagcgg gacaacgcgc
    13381 gggtcaggaa cgcggagaac tgcagtggcc atccgcgggg caagcgagcg tcggacacgc
    13441 gctgacgacg gtttgggcgc ggtaatccaa gggaagccga gttggggcgt catggccgag
    13501 tgtcaaatgc cagtagggtg caggatccga tgcgtcaagg aaacgagtaa agttgattga
    13561 ttccgaagag ttatcaggtt tttgacccat catcccaggc gcgcacctaa gtactcgttc
    13621 tgccttgggt tccggtcgac ccagggggcg ttatccgagg gaaaggcgcg attggcctcc
    13681 gcatgaggat aggggcgtaa gaccccgaaa gacctctctc gcactcgctt ataactgcaa
    13741 aatttcacat acaacgccac aaagacgcac acgcaccgag ctgcgcgcgc agcagcttcg
    13801 cgtatcgaat tgcgtttgga ggcgagcaac agaagccaaa gttgcccggt attcgcgcgc
    13861 tttgagtagc tggcgtgacc tgtgcaacga gcaatcgatc gacggaagct ccagacaaac
    13921 gagcgcacac ggccacgtcg cttcgacgct catgcgatga gcgtcgagct ctgcagactc
    13981 atcgctgcat cgcgacgagt ttggtggtct acgcgtcgtg catcgccagg agcctgacag
    14041 tagagaggag agctccgtgc cccacacact cgctcattct cgaaggtcga acggctcagc
    14101 tcggtcgggg catggggcgg cgtgctgctg aggaagcgcc attgggtgga gcctttgcat
    14161 cgggcgcagc cttcccttca cccgcctcac cttttccctt tgagtccgcc ttggaatcct
    14221 tcgggtctga tttggcatcc ttctcggtgc gccctgcctc ggctttggga tcccccgcag
    14281 ccccgcgtaa gatgccgtgc tgctgaagca cacgctgctc caaacgctcc aacagctccg
    14341 ggtgctgttc gagatattgc cgagcattgt cgcgcccttg accaatccgc tcgccgtccg
    14401 cggcaaacca cgcaccgctc ttctgaacta ggttggcgtc cacggacatg tcgagcacct
    14461 cgcccgatcg gctgacgccc tgcccgtaga gaatgtcgaa ctcgacttcg cgaaacggcg
    14521 cggccaactt gttcttgacg actttgacgc gcgtacggtt gccaatcacg accgggtctt
    14581 ttccttccga tcccgcttct ttgatggcgc caacccgacg aacatctagc cgcacggaag
    14641 cgtagaactt gagcgcgttg ccgcccgtgg tcgtctccgg acttccgaac atgactccga
    14701 tcttcatacg aatttggttc gtgaagaaga tgaggcactg cgaacgcgaa accgacccgg
    14761 tcaccttgcg caaagcttgg ctcatcaagc gagcctgtag accgacatgg gagtctccca
    14821 tgtcgccttc gatctctgct ttggggacga gagccgccac agagtcgacc accaccagat
    14881 ctacggcacc cgagcgcacc aacatgtctg cgatctcgag ggcttgctca ccgtagtcgg
    14941 gctggctgat gagcagctct tccgttttca caccgagctt tttggcatag gtgacatcga
    15001 gcgcgtgctc ggcatcgatg aaggctgcaa ctccgccgcg gcgctggacg ctcgcgatag
    15061 cgtgcagagt caaagtggtt ttaccgctcg actcagggcc gtaaatctcg atgatgcgcc
    15121 cgcgcgggta accaccgatg cccaacgcaa cgtcgagccc gagcgatccg gtcggcacga
    15181 cctggacctc tcccccaatc ttctcgccgt ccttcaggcg catgatcgcg ccgtccccga
    15241 actctttttg aacgctggcg atggccagct ccagggcttt agtcttctgc ttctcgtcca
    15301 atacgctcat gtgacctccc ggacagcgac gggaccgata tactgttcag ctgttcagat
    15361 gtcaacgaaa gggccctgag cgggacgaac gctgtgacag catttgtctt agcgaagcct
    15421 gctggtacgg atgctcgtta gctgaatcga gcagtaaacg gcaggttcgg gaggggacgc
    15481 caaggagcct gcagggggat agcactcgcc atccccctcg gctcttcccg gaagcagatt
    15541 tagtgaacga ggcggggtcc cgcttctcca ctccgtggct tcgacacggt tcggaaccac
    15601 accgttctga tcgaaacggt tcggagccga ctcagttgca ctcgctgtcg cccgccgcct
    15661 tggcgcaggt cggcgccatg acgtagtggg cgcggcagca cagctgctcg tcactgtaat
    15721 tggagcagta cgtcagacag cttgcttcgt tttcgaaggt ctcgttcgcg tcgttgtagt
    15781 cggcacagct ctcgaagaag gccgcgcaat agtccgcaca gctcatgcct ttggcgtccg
    15841 actgtccttc ggctgggctc tggcacgtat ccggggcacc cgcggcgccg ccgttggccg
    15901 tttctccgcc cgcgctcgcg ccgcccgaac cggtggcggc gccacctgtg ctcgtctcac
    15961 ccgcttggga tgtttgaccg ccctctgccg aagtcgtgcc gccttccgta gtgccttcgc
    16021 cagcttgtcc accagcgcca cccgtggcgc ccgtcttccc accggaagct ttgccacccg
    16081 tgctggtgtc atctgaatcg tccgtggtgc atccctgacc aagcccgaga cccacggccg
    16141 caaccaaaca aagactcgct acgacgtgaa agttagaaag cattttcatg gcggggagca
    16201 tcgagcgggt tcacacgagc tgtcaagtct ggaagaaacc gttcaaggac gctgcaaagc
    16261 cgagaaatgc cccggaaacg tcaactttgc agacgcctta cttcaatgcg ctcggcgcgg
    16321 cgtgcactcg cgcgcgtgac gctgaagcga tgaccttccc actccacgtt gtctccgacg
    16381 cgcggcacgc gccccaccaa ctcggcgacg aaacccgcca acgtcaccat ctcgctctct
    16441 gactcgatgt tcagcaagtc gaaaactttc cgagtttctg ctgagccacg acaggagaac
    16501 acgccggggg cgcgccgcac gatgctggga tccacacgat cgctctcgtc ttcgatctcg
    16561 ccgacgattt cttcgagcac gtcttccagc gtgacgatgc cctgggtgcc gccgtactcg
    16621 tcgacgacga tcgccatgtg ccgacgctct tcctgaaaag tacggaggac gcgctcgagc
    16681 ggagccgacg ttggcaccac cagaagcggg ctcaagagct tccgaaaatc gacgtcatcc
    16741 ccggactcac gcagcgcgaa gaacagctct ttcgccaaga ccacgccttc gactttgtcg
    16801 atgtcgccct cggccgagaa aggaaagcgg ctgtgcccag agcgccgaat caccgccaag
    16861 ttctcttcga gaccgcgatc gccggacagt gcgatcacct gtgcgcgcgg caccatcaca
    16921 tcataggcgg ttagctcgcg cagccgcgct gcgccctcga tcatgtccgc caaacggccg
    16981 cccagagcgc cttcgttgcg cccgaaggcc acgagtaacc gaatctcgtc aatcgaggta
    17041 accggtgctt cttggccccg ccgcaagagc ttggcgaaca agttggtcac ccaaatgacg
    17101 ggcttgagca ccacgaccag cgcgctcacc gtgtacgcga cgggaaccgc gaactgcgcc
    17161 acgtaggtca cgcccaacgt cttcggcacg atctcggaaa agatcagcac caagaccgtg
    17221 aacacgagcg agaagaccca gagcgtctgc tcgctgaata cgtgcacgta gatggcgccg
    17281 ctcacggacg cgccaaccgt atgcgcgacc gtgtgaaaag ccacgatagc cgcgatcgga
    17341 atatccactt cacgcttgaa gtggcgcagg atcttggcgg ccttcgattt ccccagcgct
    17401 tggatttgtg cgtgcgaaat gctgagcacg accgactcgg cgatcgagca cgcaaacgaa
    17461 accatcagcg accacagaat ggtgagggcg agcgtcaaca tggcggtcgc gcacgccctc
    17521 ccatgcgtcg cgctttcaag ccttgcgctg agagccggcc agctgttgct gttgctgctg
    17581 caggtactgc gcaaccatgc gcgagcgctc cacggcgaac tgcttcactt cgcggttttg
    17641 cgagcccgcg agcgcgttca aaagcttcgt gaccttctcc agctggcgct tctggaacaa
    17701 tgcctcgaag tagttgtgag cgaggcgcac gtcgttggcc atcttcgatt cgtggcgcga
    17761 caacacctcg atcacgcgat ccagctcgcc gcgctgcccg tagagcgccg acaaacaaat
    17821 gagagcgagt gggtcgttgt cgttccgctc gaccgccttc ttcgcccaat ccagagcctt
    17881 gtcacgcgtc tcttcattgg cggcgtagat gccctgcact gcaatgaagg gggccgccgt
    17941 cttgcgattg gcttcattgt tggcgagctg agtcacggcg cgctccgccg cttcttcgct
    18001 ttcgttgcgt tgaatttggc tgaacagata agcccaggcg tccacgctat tgggagcgag
    18061 ctcgatcgcc ttctcgacca tcgcgcgctc ttcggttggg tttcccttac cttggtaggc
    18121 cttcgccaga tgcagcagcg ggaacggatt gtcgggcaga aggttctgtg cgccgcgcag
    18181 ggtgccctcg gccttgtccc atttttcctg ctggagttgg gtcacgccga gcgcgagcca
    18241 gtcttcgccg gtgccgccca gggcgacgat cttggcgagc gtcttctccg ccttctcgaa
    18301 gcgggcgtac ttcatcaact cctgagcaaa tagacgccca cggttgagat ccgaggcgtc
    18361 ttcctgccag tgtttttcca ggcccccgag cagatcttcg aggccgatcg ccaacggacg
    18421 gccatctgcg tcctgcacct gcacggcagg gccgttgaag ccgaggagct gccacatacg
    18481 gtcgacatcg agcgcaacgg ggcccttctt catggcctcg atgagttcgt cttccggctt
    18541 cgagagcgg
//
LOCUS       DASTKU010000307         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1399102, whole genome shotgun sequence.
ACCESSION   DASTKU010000307 DASTKU010000000
VERSION     DASTKU010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399102"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFQ61_11005"
     CDS             complement(<1..402)
                     /locus_tag="VFQ61_11005"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9955026.1"
                     /translation="MAVDEDNRGTKNVLKDIYLLGYVSQVLTTTLAALAEPNRLGIVD
                     FLREGPQPVAAIGEKVGLGQPQVSKHLRVLRDAGLVLVEPRGRERHYVLRAEPLRELD
                     AWLDKFRVNWGKRFDQLDNLLRELKTKGNRNA"
     gene            complement(520..1428)
                     /locus_tag="VFQ61_11010"
     CDS             complement(520..1428)
                     /locus_tag="VFQ61_11010"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9955027.1"
                     /translation="MAHDSDRHLLDGLGVLRAVVEAGSFVGAGNRLGLTQPAVSRAIA
                     RLEARVGVRLFRRTARSISLTDEGSRFYETISPHLRAIEDATTQAGEAKAKVRGRLRV
                     NVDPGTAQEVLVPRLTPFLEQHPELFVELIVRDRLGDLVREGFDLAVRVGTPEPSALK
                     ARLLTRSRILSVASPAYLSRHGTPRRPSDIENHRCILFRDPSTGSHFGWDFVRGRKTV
                     SVNVKGKLMVNQFGLMLAACLAGEGIAQVLEFHARELLANGTLVQVLQDWADETYPIY
                     AYHHSAQLISAKVRAFLEFVVAATRA"
     gene            1656..2552
                     /locus_tag="VFQ61_11015"
     CDS             1656..2552
                     /locus_tag="VFQ61_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9955028.1"
                     /translation="MRIFVTGATGFIGSAVVRELLGAGHEVLGLARSDAGAASLQGLG
                     VEVQRGSIEDLESLKRGAAATDAVVHTAFNHDFTRFKQSCEDDRRAVEALGAALAGTP
                     RPLIVTSALGVLPKGTLLTEDVVVSPGPNAHPRSATEEAADAVAARGVRVSVVRLPPS
                     VHGDGDPHFVSMLIRHARAKGCSAYLDTGENHWSAVHRLDAAQLYRAILERRPEGGRY
                     HAVAEEGIPFRELAAAIGHRLGVPVISVARSEAVAHFGWLGNFTAMDLRASSARTRER
                     LGWNPKQVGLLQDLEQDHYFVS"
BASE COUNT          531 a          898 c          854 g          501 t
ORIGIN      
        1 tgcattccta tttccctttg tcttcagttc acgcagcagg ttgtccaact ggtcgaaacg
       61 cttgccccaa ttcacgcgaa acttgtcgag ccacgcgtcc aactcgcgga gcggctcggc
      121 acgcaatacg tagtgacgct cgcgtccgcg gggttccacc agcacgagcc ccgcgtctcg
      181 aagcacccga aggtgtttag agacttgcgg ctggccgagc cccaccttct cgccaatcgc
      241 agcgaccggc tgcggacctt cccgaagaaa gtcgacgatt ccgagtcggt tgggctccgc
      301 gagcgcagca agagtcgtcg tcagcacctg cgatacatat cctaaaagat atatatcttt
      361 caagacattc ttggttccgc ggttatcttc gtccaccgcc atcgttggcg cgctacacgc
      421 aagcgacgtc tgcacgcagt gcgccgaaga cgctaaaact cacggcagcg gggggctgag
      481 gctcccgtga acggctcgcg gcttccccga gcccagagct cacgcgcgtg ttgcggccac
      541 cacgaactcg agaaatgcgc gaaccttcgc cgagatgagc tgcgcggagt ggtgataggc
      601 gtaaatcgga tacgtctcgt ccgcccaatc ctgcagcacc tgtacgagcg tcccgttggc
      661 aagcagctcg cgggcgtgaa actccagcac ttgtgcaatg ccctcgccgg cgaggcaggc
      721 ggcgagcatg aggccgaact gattgaccat cagtttgccc tttacgttca cggacaccgt
      781 cttgcgaccg cgcacgaaat cccaaccgaa gtggctaccc gtagacggat cgcggaaaag
      841 aatacagcga tggttctcga tgtcgctcgg cctacggggc gtgccgtgcc ggctcaggta
      901 cgcgggcgac gccacgctca ggatgcggga ccgcgtcagc aagcgagcct tgagcgcaga
      961 aggctcggga gtcccgacgc gcaccgcgag atcgaagccc tcgcgcacga gatcgcccag
     1021 cctatcgcgc acgattagct ctacgaatag ctccgggtgc tgttcgagaa acggtgtcaa
     1081 gcgcggcacg agaacctcct gcgcagtgcc gggatccaca ttgacgcgaa gacgcccgcg
     1141 caccttggcc ttcgcctcac cagcttgcgt cgtcgcgtcc tcgatggctc gaaggtgggg
     1201 cgagatcgtc tcgtagaagc gactcccctc atcggtgagt gaaatcgagc gcgcggttcg
     1261 gcggaacaaa cgcactccca cgcgcgcttc gagccgtgcg atggcgcggc tcacggccgg
     1321 ctgcgtgagt ccgagccggt tgcctgcgcc gacgaagctc cccgcctcca ccactgcacg
     1381 aagtaccccc aaaccatcga gcaaatgccg gtccgaatcg tgagccatac ttctgtgcat
     1441 tcttatcatg gcgagcacga tcgttgcggg ggtctcaatg gcagtgcatt ttcttccggg
     1501 tcacgagcac agcaggccac gagatcgtcc cctaacgtgc ggccgcgggt cctttccgtc
     1561 ggcctcggta cgtcagtcgg cgatcgagct gttacccgat gcgcgggtga cgttcgcggt
     1621 cgtgtttcag ctcgttctga aagagagcca tcactatgcg tattttcgtt actggagcca
     1681 cgggtttcat cggttcagcc gtggttcgcg agttactcgg ggccggacac gaggtgctcg
     1741 ggctcgcgcg ttcggacgcg ggtgctgcat cgctccaggg gctcggtgtc gaggttcaaa
     1801 gaggctcgat cgaagacctc gagagtctga agcgaggtgc cgccgcgacg gatgccgtag
     1861 ttcacaccgc gttcaaccat gatttcacca gattcaagca gagctgcgag gacgaccggc
     1921 gcgccgtcga agcgctcggg gccgcacttg caggaacccc gcggccgctc atcgtgacat
     1981 ccgccctcgg ggtattgccg aagggcaccc tcctcacgga ggatgtcgtc gtatcgccgg
     2041 ggccgaacgc gcatccacgc tccgcgaccg aggaagccgc ggacgcggtc gcagcgcgtg
     2101 gggttcgggt gtccgtcgta cgattaccac cctcggtaca cggggacgga gatccgcact
     2161 tcgtatccat gctgatccgc cacgcgcgag cgaagggctg ctctgcctac ttggacacgg
     2221 gagaaaacca ctggtccgcg gtgcaccggc tcgacgccgc gcagctctat cgcgccattc
     2281 tcgagcggcg acccgaggga ggccgatatc acgcggtggc cgaggagggc atcccgttcc
     2341 gcgaacttgc cgcagccatc ggtcaccgcc tcggcgtgcc cgtcatcagc gtcgcgcgca
     2401 gcgaagcagt ggcgcacttc ggttggctcg gcaacttcac ggcgatggac ctccgggcgt
     2461 cgagtgcacg cacgcgcgag cggctcggct ggaacccgaa gcaggttggc ctcctgcagg
     2521 acctcgaaca ggatcactac ttcgtaagct gaaatggtca aagctgtgca cccgctcaga
     2581 acgagcgccg tgggtctatg ggcgaaacct aaacctctcc taagctcgac ctaaagaccc
     2641 tcaccctttg ggccctcatg atgtccccag atgcgagcac tgaacgcaaa caagggagag
     2701 gtcatgaaga acgaatgctc gagcgctggt ccaaacatta tatccatggg ctcgattttc
     2761 acctggagca ggggcctgcg ttct
//
LOCUS       DASTKU010000308        16259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1844789, whole genome shotgun sequence.
ACCESSION   DASTKU010000308 DASTKU010000000
VERSION     DASTKU010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16259
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1844789"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFQ61_11020"
     CDS             <1..460
                     /locus_tag="VFQ61_11020"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955029.1"
                     /translation="PGTGAYMSPEQVRGEELDPRADLYSAAVVLYEMVCGTTPFDTGQ
                     RDELMLRAAQIEEPPPPLSLRVDGIPGELEMVLARALAKDRRHRFTTALELGEAVRKA
                     MGQAPAANWAAQLEFSALAKTISAAMPQQSDAQIAQRAERLRTAMMSPAR"
     gene            672..2822
                     /locus_tag="VFQ61_11025"
     CDS             672..2822
                     /locus_tag="VFQ61_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04551 family protein"
                     /protein_id="HET9955030.1"
                     /translation="MSIRTCMVLSAFASLLAANSALAQNAAPKPPPAPKAPAAAAPAP
                     AAPVPAPNAPAEGAPARPELPADAGAVGDEEAAGAEPGAVPAAPAAPTAATPPSRTVL
                     PAFPEPGNDARTLSRMGTERPEAAKAKEESDVFAEDWWSHVRPVVELHGNFRVRSELF
                     YRFSLGRVDPPNAEGILWPRPPDSPYASDPTNPYNSDPPRLCTADEAGTGDSSSLSSP
                     VPCRSNTQAGANMRFRLNPEIHISDNLRILSQIDLLDNLVLGSTGMGTASGSPDASLD
                     IHNAARAVDATMDTQNATVSGTRNSFSDTIRVKRAWAEYMSPVGALRFGRMPNHWGLG
                     MVNHSGDGYDDDYQSTIDRVQFIAGIKPLDLYASAAWDFPYEGPTSAVIGRTIPREYD
                     LGQLDDVDQYTFSIARRKSPELLRLALTRGEVVVNGGLQLTYRKQLLQAQTATTALQT
                     SGIPLEYVRRDSHYYLPDLWLQVLYKQFRFETEIATVQGSIANPDQSPSPINTDRDLT
                     RPRFKIRQWGFVGEIEQRLIEDRLRFGAGFGWASGDPDEESMIAASSGNTFGQLGGDR
                     TLSSFRFNPAYRVDLILFRNLLTRVQGAYYVRPNISYDFVHDSTGQRIGGSIAGIWSR
                     ASQFVQAPGHDRDLGIEINGALYYQSKDGSLNDDRNRMGGFYARLEYGILFPMAGLGY
                     GNVRAADLNGKLNRNDAASTSAAQTLRLFLGAAF"
     gene            2966..3904
                     /locus_tag="VFQ61_11030"
     CDS             2966..3904
                     /locus_tag="VFQ61_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955031.1"
                     /translation="MNRRLPLTRFRRFAGIVCAWWLCAACSSEDIASSPASGGQTNSG
                     SGGQTAASGGTSGGSTASCDPGPGYQTDESPQDVRRVTAKLLDASGAPLAQELVQVCG
                     LDICVSGKTGNDGSIAIQTQQAIKRPALKFGEGLNSARFAWLIPKSEPEFDAGEVRGV
                     RLPSLGESVPLSAGETATQGGLSLKLAAGTSIAIDVLTFQKPAEKGLRAVRVPLDEAP
                     EAVPRDRGFELLYAATPTETHFCPPAELSVENSENWEPGTEVEVFLHGVDVRQEFAPY
                     GEFAKVSDARVSEDGKSIVTTEPGLPVLGVLAFKRK"
     gene            3915..4571
                     /locus_tag="VFQ61_11035"
     CDS             3915..4571
                     /locus_tag="VFQ61_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955032.1"
                     /translation="MRSLASSSACLVLGGLLSACGSNRPELPAGFELSVGCSAPDYPL
                     GPYGTEPGSVVKNACFPGLRDPARSGRTESELEALSLADFYDPSGERYAVLLLNTAAV
                     WCSACRVEHEDLSGRLANLSGRKLGVLGALFQDRDRNPAQFSDLALWTETFATPFPMA
                     LDPSYQLGVYASAETAPLNLVIDTRDMRILQKYIGDQAEVLWPYLDSILPGSAASANP
                     "
     gene            4582..6486
                     /locus_tag="VFQ61_11040"
     CDS             4582..6486
                     /locus_tag="VFQ61_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955033.1"
                     /translation="MRLRVGASAAGSGHVAAAWLVASTLSCWPSLGFAEESRPLVQFT
                     QASSVLYNFDNRDTRPGQVGSLANDHSGMLYNRSNLQVTSGRWTGALRVDQVWFFASP
                     EPLGIARQLQSLERFGDEASRQEFERNKIAEAGLELSNRYINWVYPAQYRLTYTTPEL
                     ELSAGDGYAQFGRGLVLSLRKLDEIASDTTLRGLRVTARKRVGSARIQATALGGSLNP
                     LRIDEASGRYLGVNSSVTPGWLAITEAGMPRAVHTDFTSDSNDCSTLATCSYAPDRLV
                     GGQFLLDVSGVTLGTQAAWLVRGVPLSNDLVRTAQSVGIASQSFEIPSFAKHGSLYLE
                     GAVQKLSRRDQDAGTDFRMGHAIYGTLSWVEPRFSLLFEAKHYRRFFPLAANVSNARV
                     REFSLLQYNAPPTTEESWNDTQFGGLNTCVSGARLKGQVFATRAHAFYGWVSHYQSFA
                     ESVRNERCDTARENANLIWDTAIGLDLRPSSRRARWDLNLGVRFDDTHRELAGPSGPT
                     TVFYRELALRYNITEPLSAKVSLELQGVHRRRRETVGGPEQDFVEGRHSTAFEWGERF
                     GAGIGLEYDTRPDTPHGYVNGMVTYRPSDAWSLALFAGQRRGALRCVGGVCRVYPPFE
                     GVRLDATFRY"
     gene            complement(6539..7135)
                     /locus_tag="VFQ61_11045"
     CDS             complement(6539..7135)
                     /locus_tag="VFQ61_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9955034.1"
                     /translation="MSQIARVASFIGLLAVGSCSCTPAGSTNAIPQGSAPAANEAGSV
                     RQVGPAQAPPDFELRALDGSSVRLSDYLGKQVVLIDFWATYCEPCLRAMPTLDALYRK
                     HRARGFVVLGVAIDPADSISEVRADVQKLGVSFPVLLDQDTRVVAAYNPKTSAPFSVL
                     IGRDGRILRKKEGYSSDSGANLERDIRGALDQQVASGP"
     gene            complement(7132..8073)
                     /locus_tag="VFQ61_11050"
     CDS             complement(7132..8073)
                     /locus_tag="VFQ61_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955035.1"
                     /translation="MSRLSPALPAYYLLSALTSTNQDDFKLAAAFRSIMPRFSRHTFG
                     VLGPLITLLALGGSSSLIWGCSSEDLASETGGASAIANTGANTNTGGASGTAQAGTDR
                     GGTATTSAGGGNTGGVLGSRVSEPARVDYPSGPYGRGIGATISNLSFLGFRAPTQAGY
                     DLSKLEVVRLSDFYNPGGSRNDVKVIVLNSSAVWCSVCRAEYQRLQASDIYAKYRSAG
                     IEMVGLLFEDQDANPAQPSDLVLWGRDILVPRYKAVEFPLLLDPGFKSGVYFTSDATP
                     MNMLIDAHTMQILNITMGFDVSGADDYFERLLEWTQR"
     gene            8087..9442
                     /locus_tag="VFQ61_11055"
     CDS             8087..9442
                     /locus_tag="VFQ61_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955036.1"
                     /translation="MKFSTLPVVLLLLSILPAASCGSGEAQPACRDLDCKTPERCVEG
                     ACRPPCEGQDDCPIGQNCVEQNLDSGNPQSFCVVLDYARNGSTGQQEPCTADSQCDSL
                     RGYRCQAGRCASTTAPTQTPKLFTSCEKDADCDLTRGHQCIEGACRVACSGHFDCTPI
                     GLCESVAGGTYCSQGPAAKPGQYYASCVNGTADCDSSAGFVCVGSGVGDLDAYCTTDC
                     AGTADCPTGFACETLGATPCEEACGLQGQAVAGCVKSSEIGAGRRYHCQQPIGLVRSV
                     CRKREFCDTCERDEDCRSVPGGICARDQSGAKNCTVECDPSVDSCPWGAATECGLWDE
                     QRKLFTCAHRFGSCRGRGNACDPCANTADCMGKGYCTQASFTGEQFCIDLTLECDCGA
                     DADASGTCTGHGCPDSPGGRPMLCYSDSRFPGDPLSHRCVAAGSGGNPIGPSQSGCWV
                     R"
     gene            9705..10868
                     /locus_tag="VFQ61_11060"
     CDS             9705..10868
                     /locus_tag="VFQ61_11060"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9955037.1"
                     /translation="MSAPLPSQPETVLDGKYRLGQTLAVSSFGAVYDAEDLSVGKRVA
                     LKVLHRALDADGRTTFFNASRAVARLSHPNLVSILAIGGSDEHAPYLVMEHLAGETLQ
                     ELVTREGPLSALKLADLALDLFAGLAALHAGGVLHGNLSPRNVFVTYPRPQRPQLKLL
                     GLGVPSACREPTREPGYMRAASQLGAATTTERSDVYAASAILFFMATGRAPTSPIDPL
                     MTPLPDSIRECVSQGMGSDPLVTPSSAADLMQQLRQRFSNSADALSQSGRQPLLREGY
                     NSVLQTRTLDVLGAAASGAPRPALLRAAASDRLVSDSLLLSPRVPKPPGSPKLELGLD
                     FMPSPGDPVLQAELEKRHLPTEVPVKSRWFGALSATALGFTVGVALARLLGLI"
     gene            10911..11795
                     /locus_tag="VFQ61_11065"
     CDS             10911..11795
                     /locus_tag="VFQ61_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET9955038.1"
                     /translation="MADLQDLSIPTRPIQPDDPVPEVKNIILVMSGKGGVGKSTVATN
                     LSLALQATGKSVGLLDADIYGPSIPTMLGIAGRPMSNGEKIEPLERYGLKLMSIGFML
                     DDPKSAVVWRGPMLHGALLQFLKDVAWGPLDFLLLDLPPGTGDVALTLSQKVRASGAV
                     VVTTPQEVALQDVYKSVSMCQKVGIPILGVVENQSYFLCDGCNKRHELFGAGGGAKIA
                     EYAEAPLLGQIPLNPAVREWGDAGTPIVHAAKGSPAAQAFMDVAARLLETMAAQGEVD
                     GLAIDRSGGKNRHLPITR"
     gene            12210..14006
                     /locus_tag="VFQ61_11070"
     CDS             12210..14006
                     /locus_tag="VFQ61_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET9955039.1"
                     /translation="MSHEQSDTPDIAQSNSASSESAAGESAAGNAAATVNTPAEGAAA
                     SSSEGGSTEARAAEAAPVEGGDEGEDGEETDAEGASEGEAGKEAASADGVAKKKRRRK
                     RKKKKPEGAAAHAPGSEGEPRESTRPHRASERAPFHVGEEVFGKVTAVIDTAIMVDLS
                     GKALAIFDRSEMEPDDLVPVPGERFVARVHNDGTRGGLVVLTRKPLREEETKAKLEAA
                     FKNGELVSGLITGVIKGGVEVDIGGIRAFAPASGMDLHPGQANFTTLLGQRMDFKLVS
                     VDKSGREVVVTRRPMLEAEAHERRKRALSLLTEGQVLPGVVRTVVEWGAFVALPEAEN
                     LEGLVHVSEVSHDPRAPITELVKPGDRFEVKIIKIDEKGKIWLSRKALVDDPWASARR
                     KFEVRSRHTGKVTRLEKFGAFVELEPGIEGLLHVADLSLERVEHPNLVLKEGQDLEVL
                     VHHFDPRSKKITLHPAPPADRPEEAPQKILRNALIKAEVVKGEAAGVVVRVLGVTGRA
                     ARGFIPAGQTGTNRGTDLRKAFKPGSVIDVKVLDIDPRSGEPKLSIRGFKEDEERRAH
                     NEYRQKLKEEGGFGTLGDLLKNKLRAGEEREV"
     gene            14176..14760
                     /locus_tag="VFQ61_11075"
     CDS             14176..14760
                     /locus_tag="VFQ61_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9955040.1"
                     /translation="MSEQTDRELVEAAQNGDAEAFGVLMRRHQRRVYRLALHLVRNAA
                     EAEDVTQETFVRAYGALDRFDGRSEPFTWIYRIAVNLSLNAIRARKPTDRSAPPDDPR
                     VEPLLVERRAKLASPEAASEDKELAQALLSSIDRLSDVLRTTLVLVTVDGLPQADVGR
                     ILGCSEGTVAWRVHEARRKLREYMSELGHGENDG"
     gene            14753..>16259
                     /locus_tag="VFQ61_11080"
     CDS             14753..>16259
                     /locus_tag="VFQ61_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955041.1"
                     /translation="MAERSPSLGDRAEALLSSFGMPEFDEEALAARILARLDPSARGS
                     TDSALLQPPLPDDAGEPGALPLSVRAELLDLESHSRLLPADVLQPVVRAPDVVSVPAL
                     SPNELSPGALPAPLVPAGVGSGNTLASDGERPTEVTELPAGLSLSGSSEGTSAAGLPG
                     AGLPGAGLPGAESGAAALLRPALGGLTPAPPPSGQSLAALARAVAQSGSLGKGARSQV
                     TDIAKESLSVAAAARAQTDAIVERVRSAEKAPESVPPPATPARVLPATPASLTTATTA
                     RSAPPPRTRSPGNWQWLALAGTLALAAGVVLVLRGSEKSASAPPALAETRAQAASPSP
                     SLGAGPAPVEGALGEPHAASASPSDAPAVAAHEASAPQAEARAALAIAPRSAANSGSV
                     ERTNPGFGADGAASPERPLARAPSSARPARPQPESIVLEETPTASQPDANSATNPEAH
                     LKPAAGSAAAGGLPDKPATGAVQAALGSVMGAARACVAGAATPTPAQIVFGS"
BASE COUNT         2932 a         5209 c         5298 g         2820 t
ORIGIN      
        1 ccccggaacg ggcgcataca tgtccccgga acaggtgcgg ggcgaggagc tcgatccgcg
       61 cgccgatctc tactcagccg ccgtggtgct gtatgaaatg gtttgcggca ccaccccgtt
      121 cgacacgggc cagcgcgacg agttgatgct gcgagccgct caaatcgagg aacccccgcc
      181 ccctttgagc ctgcgcgtgg acggcattcc gggcgagctc gagatggtgc tggcgagggc
      241 tttggccaaa gatcggcgcc atcgcttcac gactgctctg gagctgggag aggccgtgcg
      301 taaagccatg gggcaagccc cggccgccaa ttgggcagct cagctagagt tctccgcgtt
      361 ggcaaagacg atttcggccg caatgcctca gcagtccgac gcgcaaatcg ctcagcgggc
      421 cgaacgattg cggaccgcga tgatgtctcc cgctcgttga gcggccatcg agcggccatg
      481 ccgcgttact tgtggcgact gcagggtaag ttcggaccag gaccaagagg ctcaccccca
      541 gctaggccgg tcgctccgcc cgcagaagcg agccgctccg aatctcccct cttgccctcc
      601 cctgccccgc atgcgaaagg accgctcgga cttgcccggg ttttagcgcg gtgaggcctc
      661 ggattttgac gatgtcgatt cgtacttgca tggttttgag cgcgttcgcg tcgcttctgg
      721 cggcaaactc cgctctcgca cagaacgcag ccccgaaacc tcccccggct cccaaagcgc
      781 ctgcggctgc agcgcccgct cctgctgctc ctgttccagc acccaacgcc cccgcggagg
      841 gtgcgcccgc ccggccagag ttgccggcgg atgccggagc ggtaggggat gaagaagccg
      901 ctggcgcaga acccggagca gttccggcag ctcctgcggc acccacggcg gcgacgcctc
      961 cgtcccgcac cgtgttgccg gcgtttccgg aacctggcaa cgacgcacgc acgctcagcc
     1021 gcatgggcac agagcggccc gaagccgcca aggccaaaga agagagcgat gtgttcgcgg
     1081 aagactggtg gagtcacgtg cgccccgtcg tggagctgca cggaaacttc cgcgtgcgca
     1141 gcgagctctt ctatcgattc tcgctcggtc gagtcgaccc gccgaacgcg gagggaatcc
     1201 tgtggccgcg tccgcccgac agtccttatg cgagcgatcc cacgaacccc tacaatagtg
     1261 atccgccgcg cctctgcacc gcagacgaag caggtacggg ggacagctct tcgttgtcgt
     1321 cgccggtccc ctgccgcagc aacacgcagg ccggcgccaa catgcgcttc cggttgaatc
     1381 ccgagatcca catctcggac aacctgcgca ttctgtctca gatcgatctg ctggataatc
     1441 tggtgctcgg ctccaccggc atgggtacgg cgagtggttc accggacgcg tctttggata
     1501 ttcacaacgc cgcgcgcgcc gtcgatgcga cgatggatac ccagaacgcg accgtcagtg
     1561 ggacgcgcaa ctcgttcagt gacacgatcc gcgtcaaacg agcctgggcc gagtacatga
     1621 gccccgtggg tgcgttgcgc ttcggacgca tgccgaacca ctggggtctc ggcatggtga
     1681 accactccgg agacggctac gacgacgact accagtcgac gatcgatcgg gtgcagttca
     1741 ttgcgggtat caagccgctc gatctgtacg cctcggcggc ttgggacttc ccgtacgagg
     1801 gacccactag tgcggtgatt gggcgcacga ttccgcgaga gtacgatctg ggacaactcg
     1861 acgacgtcga ccaatacacg ttctcgattg cacgccggaa gagccccgag ctgctgcgtc
     1921 tagcgctcac gcgcggcgag gtggtggtca acggcggcct ccaactcacg taccgcaagc
     1981 aactgctgca ggcccagacc gcgacgacag ccctgcagac ctccggcatt cccctcgaat
     2041 acgtgcgacg ggactcgcac tactacctgc ccgatctctg gttgcaggtg ctctacaagc
     2101 agttccgttt cgagacggag atcgcaacgg ttcagggttc catcgcgaac cccgatcaaa
     2161 gcccgagtcc gatcaacacc gatcgcgacc tcacgcgccc ccgcttcaag atccgtcagt
     2221 ggggcttcgt gggcgagatc gagcaacggc tgatcgaaga tcgactccgc ttcggcgccg
     2281 gcttcggctg ggcctccggt gatccagacg aggagagcat gatcgccgct tcgtccggca
     2341 acaccttcgg gcagctcggc ggggaccgca cgctctcgag cttccgcttc aaccccgctt
     2401 accgcgtcga tctgattctg ttccggaacc tgctgacccg agttcaaggc gcctattacg
     2461 tgcgcccgaa catctcctac gatttcgtgc acgacagcac cggacagcgc atcggcggca
     2521 gcatcgcggg gatttggtcg cgagccagcc agttcgtgca ggcgccggga cacgaccggg
     2581 atctcgggat cgagatcaac ggcgcgcttt actaccaatc caaggatggt tctctgaacg
     2641 acgaccgcaa tcggatgggt ggtttctacg cgcggctcga gtacggcatc ctgttcccga
     2701 tggccggttt gggctacggc aacgtgcgcg ctgcggatct gaacgggaaa ttgaatcgta
     2761 acgacgcggc cagtacctcc gcggcccaga cgctgcgctt gttcctcggc gccgcattct
     2821 gacggtccga atccacccgg cgcgctttga atcttcgcgc gccgggcttc gggcgtgccg
     2881 aagcaagaag cttggcgatt tggcgtgccg tgagtgatat ggggggttcg gggcttcccg
     2941 cccgggtttc cgctatactg ccggcgtgaa ccgacgactt ccgctcactc gcttccgccg
     3001 attcgcgggt attgtttgtg cctggtggtt gtgcgccgct tgcagcagtg aggatatcgc
     3061 gtcgagcccg gccagcggcg ggcaaaccaa ctctggcagc ggcggccaaa cggccgcgag
     3121 cggtggtacc agcggaggct ccaccgcgag ctgcgatccg ggccccgggt atcagacgga
     3181 cgaaagcccg caggacgtgc ggcgcgtcac cgccaagttg ctcgatgctt cgggagcccc
     3241 gctcgcgcag gagctcgtcc aggtctgcgg actcgacatc tgtgtctccg gtaagaccgg
     3301 caacgacggt tcaatcgcga tccagacgca acaagccatc aaacgccccg cgctcaagtt
     3361 cggcgagggc ttgaatagcg cccggttcgc ctggctcatc cccaagagtg aaccggagtt
     3421 cgacgccggc gaggtgcgtg gagtgcgttt gccgtcgctc ggtgagagcg tgccgctgag
     3481 cgccggcgag accgcaactc agggcggcct gagcctgaag cttgcggcgg gaacgagcat
     3541 cgcgatcgac gttttgacgt ttcaaaagcc agctgagaag ggcttacgcg ccgttcgggt
     3601 gcccttggac gaagcgcccg aggcggttcc gcgagaccgc ggcttcgagc tgctctacgc
     3661 cgcaaccccg accgagacgc acttctgccc gcccgccgag ctcagcgtgg agaacagcga
     3721 gaactgggaa ccgggcacgg aagtggaagt gtttttgcac ggcgtggacg ttcgccagga
     3781 gttcgcgccg tatggcgagt ttgccaaagt gagcgacgca cgcgtgtcag aagacggaaa
     3841 gagcatcgtc accaccgagc ccgggttacc ggtactcggc gtgctggctt tcaagcgcaa
     3901 gtaggcgacc gccgatgcgc tcgctcgctt catcctcggc gtgtctggtg ctcggcggtt
     3961 tgctgagcgc gtgcggctcg aaccgtccag agctacccgc cggtttcgag ctcagcgtcg
     4021 gctgctcggc gccggactat cccctcgggc catacgggac agagcccggg agcgtggtga
     4081 agaatgcgtg cttcccgggg ctgcgcgatc cggctcgcag cgggcgcacc gagtcggagc
     4141 tcgaagcgct ctccctcgca gatttctacg atccctcggg tgagcgctat gcggtgctgt
     4201 tactcaacac ggccgccgtt tggtgctcgg cgtgccgcgt ggaacacgaa gacctcagcg
     4261 ggcgtttggc gaacttatcg ggccgcaaac tgggggtact gggcgcgttg tttcaggatc
     4321 gggatcgcaa tccggcgcaa ttttccgacc tcgcgttatg gaccgaaacc ttcgccaccc
     4381 ccttcccgat ggccctggat cccagctatc aacttggcgt ctacgcttcc gcggagaccg
     4441 ccccgttgaa cctggtgatc gacacgagag acatgcgcat tttgcagaag tacatcgggg
     4501 atcaagccga agtgctgtgg ccgtatctgg attcgatatt gccgggctcc gccgcgtcgg
     4561 cgaacccctg aggcccgggg agtgcgcctc cgcgtcggtg cgtctgccgc tggctcgggc
     4621 cacgtcgctg cagcttggct cgtggcgagc acgttatctt gctggccatc gcttggcttc
     4681 gcagaagagt cgcggcccct cgtgcagttc acgcaagcct cgagcgtgct ttacaacttc
     4741 gacaaccgcg acacgcgccc aggacaggtg ggcagcctgg ccaacgatca ctcgggcatg
     4801 ctctacaacc gttccaacct gcaggtcacc tcgggccggt ggacgggcgc attgcgggtg
     4861 gatcaagtgt ggttctttgc gtctccagag cccctcggca tcgcgcggca gctccagtcc
     4921 ttggagcgct tcggtgatga agcctcacgc caggagttcg aacggaacaa gatcgccgag
     4981 gcggggctgg agctctccaa ccggtacatc aactgggtct atccagctca gtatcgactg
     5041 acatacacca ctcccgaatt ggagctctcg gcgggggacg gctatgccca attcgggcgc
     5101 ggcttggtgc tcagcctccg caaactggac gagatcgcga gtgacacgac cttacgcggc
     5161 ttgcgggtca ccgcacgcaa gcgtgtgggc agcgcgcgca tacaagccac cgcgttgggc
     5221 ggctctttga acccgctgcg aatcgacgaa gcgagcggcc gctacctggg cgtgaactcc
     5281 tcggtaactc ccggttggtt ggcgatcacc gaagccggga tgccgcgcgc ggtgcacacc
     5341 gatttcacga gcgacagcaa cgattgctcc acgctggcca cctgcagcta tgcccccgac
     5401 cgtctcgtgg gtgggcaatt cctcctcgat gtctccggtg tcaccttggg gacgcaggcg
     5461 gcgtggttgg tgcgcggcgt gccgctctcg aatgacctgg tgcgaaccgc gcaaagcgtc
     5521 gggatcgcca gccaatcctt cgaaattccg tctttcgcca agcatggctc gctctacttg
     5581 gaaggggccg tccaaaagct ctcgcgccgc gatcaggacg ccggcacgga ctttcggatg
     5641 ggtcatgcca tctacggaac gttgagctgg gtcgagcccc ggttctcact gctcttcgag
     5701 gccaagcact atcggcgctt cttcccgctc gcagccaacg tgagcaacgc gcgcgtacgc
     5761 gagttctcgt tgctccaata caacgcgcct ccgaccaccg aggagtcctg gaacgatacc
     5821 cagttcggcg gactcaacac ttgcgtctcg ggagcgcggc tcaaggggca ggtattcgcg
     5881 acccgcgccc acgccttcta cggctgggtc tcgcattacc agagctttgc cgagtcggtc
     5941 cgcaacgagc gctgcgacac ggcgcgcgag aacgccaacc tgatttggga cacggcaatt
     6001 ggtctggact tacgcccgag ctcacggcgc gcgcggtggg acctcaactt gggtgtgcgc
     6061 ttcgacgaca cgcatcgaga gctcgcgggc cccagtgggc caactacggt gttttatcga
     6121 gagctcgcgc tgcgctacaa catcaccgaa ccgctcagcg ccaaagtgag cttggagtta
     6181 caaggcgtgc atcgccgccg gagagagacc gtgggtggcc ccgaacagga tttcgtcgag
     6241 ggccgtcatt cgaccgcgtt cgagtggggt gagcgctttg gggcgggcat cggtttggaa
     6301 tacgacacac gccccgacac accacatggt tacgtgaacg gcatggtaac gtatcgacct
     6361 tcggacgcct ggagtttggc actttttgcg ggacaacggc gcggcgccct gcgctgtgta
     6421 ggcggcgtgt gccgcgtcta cccaccgttc gaaggcgtgc gcctggacgc gacgtttaga
     6481 tactgatggc acgattgcca taggcactgc tgccattcgg cagctccgga ttcatcgact
     6541 agggaccgct cgcaacttgt tgatccagag cgcctcgaat gtctcgctcc aggttcgcgc
     6601 cgctgtcact cgagtagcct tctttcttgc gcaagatgcg gccgtcgcgc ccgatcaaca
     6661 cgctgaatgg tgccgaggtt ttcgggttgt atgcggccac cacgcgcgtg tcttggtcca
     6721 agagcaccgg aaagctcacg ccgagtttct gaacgtcggc ccgcacctcc gagattgagt
     6781 cggccggatc gattgcgacc ccgagcacca cgaacccgcg cgcacgatgc ttgcgataca
     6841 acgcatccag cgtcggcatc gcccgcagac acggctcgca gtaagtggcc cagaaatcta
     6901 tcaggacgac ctgcttgccc aagtagtcag acaggcgaac gctcgaacca tccaaggccc
     6961 gaagctcgaa gtccggggga gcctgcgccg ggccaacttg gcgcaccgag cccgcttcgt
     7021 ttgcggccgg cgctgagccc tgcgggatcg cgttcgtgct tccggcgggg gtgcaggagc
     7081 acgagccgac cgcaagtaaa ccaatgaaac tcgcaactcg tgcgatctgg ctcaccgctg
     7141 ggtccattcg agcaagcgtt cgaaataatc gtcggcccca gagacgtcga agcccatcgt
     7201 gatgttcaga atttgcatcg tgtgggcatc aatcaacatg ttcatcggag tggcgtctga
     7261 cgtgaagtag accccagact tgaaccctgg atcgagcagc aacgggaact ccaccgcctt
     7321 gtagcgcggc accaagatgt ccctgcccca cagcaccaga tcgctcggtt gagctggatt
     7381 ggcatcttga tcttcgaaca gcaaccccac catttcgatg cctgcgctcc gatacttggc
     7441 atagatatcc gaggcttgta gccgctggta ttcggcgcgg cacacgctgc accagaccgc
     7501 cgaggaattg agcacgatga ccttcacgtc attgcggctg cccccgggat tgtaaaagtc
     7561 cgagagccgc acgacttcga gcttggatag gtcgtatccg gcctgtgtcg gcgcgcggaa
     7621 acccaaaaac gacaggttcg aaatcgtagc gccgattcca cgaccgtacg gacctgacgg
     7681 gtaatccact cgagccggct cggacaccct actacccagc actcctcccg tgttcccgcc
     7741 cccggcggag gtcgtagccg taccgcctcg atccgtgcca gcctgagccg tgccgctcgc
     7801 gccgccggtg ttcgtgttgg ccccggtatt tgctattgcc gaagcacctc ccgtctcgct
     7861 cgccaggtct tcactggagc aaccccagat gaggctcgat gagcctccga gcgcgagcaa
     7921 ggtgatcagc gggccaagca caccgaacgt gtgtctcgag aaacgcggca tgatgctccg
     7981 aaatgcagct gcgagcttga agtcgtcttg gttcgtggaa gtgagcgccg agagcaagta
     8041 gtatgcgggc agagctggac ttagtctgct catagccgga attctcatga aattctcgac
     8101 tttgcctgtg gtcctgctgc ttctttccat cttgccggca gccagctgtg ggtcgggcga
     8161 ggcccagcct gcctgccgag acctggattg caagacgccc gagcgctgcg tggagggggc
     8221 ttgccggcca ccttgtgaag gtcaggacga ttgcccgatt gggcaaaact gtgtcgagca
     8281 aaatctggat tcaggcaacc cgcaaagctt ctgcgtagtg ctcgactacg cgcgcaacgg
     8341 cagcacgggc caacaagagc cctgcaccgc ggactcacag tgcgactcgc tgcgaggcta
     8401 tcgctgtcaa gcgggccgct gcgcgtcgac gactgcgccc actcaaactc cgaagttgtt
     8461 cacgagctgc gagaaagacg ccgattgtga tctcacgcga ggccaccagt gcatcgaggg
     8521 ggcgtgccgc gtcgcgtgca gtggccactt cgactgcacg ccgattggtc tctgcgagtc
     8581 agttgccggc ggcacgtatt gcagccaagg gcccgctgcc aagcccggtc aatattacgc
     8641 gagctgcgtg aacggcaccg ccgactgcga ctccagcgcg gggttcgtgt gcgtcggcag
     8701 cggcgtggga gacttggatg cttactgcac gacggactgc gcgggaacgg cagattgccc
     8761 cacggggttc gcctgcgaga cgcttggagc tacaccgtgt gaggaggcct gcgggctgca
     8821 agggcaggcc gtcgcgggct gcgtcaagag ctccgaaatc ggcgccggtc gccgctacca
     8881 ttgccagcag ccgattgggc tggtgcgcag cgtgtgccgc aagcgtgaat tttgtgacac
     8941 ttgcgagcgc gatgaagatt gccgcagcgt tcccggcggg atctgtgcgc gcgatcagag
     9001 cggcgcgaag aactgtacgg tggagtgcga tcccagcgtg gactcgtgcc cttggggtgc
     9061 agcaacggag tgcgggctgt gggacgaaca gcgcaagctc ttcacgtgcg cacaccggtt
     9121 tggcagttgc cgcggacgcg gcaacgcctg tgatccctgc gccaacacgg cagactgcat
     9181 ggggaagggc tactgcaccc aggcgtcgtt cacgggtgag cagttctgta tcgatctcac
     9241 actcgagtgt gattgcgggg cagacgccga cgcgagcggc acgtgcacgg ggcacggctg
     9301 ccccgattca ccgggaggcc gcccgatgtt gtgttactcc gacagtcgtt tccccggtga
     9361 ccctttgagc caccgctgcg tagccgcggg cagcggtggg aacccgatcg ggccgagcca
     9421 gagtggctgc tgggtgcgct gactttttgc ggcggagcgg ggggccggcg cggtggcgcg
     9481 gggctgaggt cgcgcctggt ctgcgctcgg gtagccaacg aacgagcttc gcgcacgtta
     9541 catcgtggcc aacgaaaagc tacgcttcac agccgtcgtt gcaggggtga ctcgagtcca
     9601 aatgtcccgt cgcgaggcgt tctggatggc ccgtccgcag catgccggac gcgcccctgc
     9661 attgctaaag gccagcgaca atcagccgtt cagcgagaac ggtgatgtcc gcgccgttac
     9721 cgagccaacc ggaaaccgta ctcgacggca agtatcgcct tggccagacg ctcgcggtct
     9781 cgagctttgg ggccgtctac gacgctgagg acttgagcgt tggtaagcgg gtggcgctca
     9841 aggtgctcca ccgcgccttg gacgcggatg gccgaacgac attcttcaat gcgagccgcg
     9901 cggtggctcg tctgtcccac cccaatctgg tctccatctt ggccatcggc ggcagtgatg
     9961 agcacgcccc ctatttggtg atggagcacc tggcggggga aacgcttcag gagctggtga
    10021 cccgagaggg gcccttgtcc gcactgaagc tggcagatct ggcactggac ctgttcgccg
    10081 gactggcggc gctgcacgcg ggcggggttc ttcacggtaa cctttcgccg cggaacgtct
    10141 tcgtcacgta cccgcgcccg cagaggcctc aattgaagtt gctggggctg ggcgtgccct
    10201 cggcgtgccg agagcccact cgagagcctg gatacatgcg tgcggcgagc cagctcgggg
    10261 cagcgacgac gacggagcgg agcgacgttt acgcggccag cgcgattttg ttcttcatgg
    10321 cgaccggacg cgctccgacg tcgccgatcg acccgttgat gactccgcta ccggactcga
    10381 ttcgagaatg cgtctcgcag ggcatgggaa gcgacccctt ggttactccg agcagcgcgg
    10441 cggatctgat gcaacagctg cggcaaagat tctcgaacag cgccgacgct ctgagccaaa
    10501 gcggccgtca gccgctgcta cgggagggct acaactcggt gctccagacg cgcacgctgg
    10561 acgtgctggg cgccgccgcg tcgggagcgc ctcgcccggc cctgctccga gcggccgcga
    10621 gcgaccggct ggtgtccgac tcgctgctgt tgtcgccgcg ggtcccgaag ccacccgggt
    10681 ctcccaagct ggagctgggc ttggacttca tgccgagccc cggcgacccg gtactgcagg
    10741 cggagctgga aaagcgtcat ttgccaaccg aagttccggt gaaaagtcgc tggttcggcg
    10801 cgctctccgc cacagccttg gggtttaccg tcggcgtggc tctcgctcgg ttgctcgggc
    10861 tgatttgaca gcctgaccgg caaggcttac agccgtgccg cgagctagcc atggctgacc
    10921 ttcaggactt gtcgattccc acccgcccca ttcaaccgga cgaccccgtg ccggaagtca
    10981 aaaacatcat cctggtgatg agcgggaagg gcggagtcgg aaagagcacg gtcgcaacga
    11041 atctgtcgct cgctctgcag gccaccggca agagcgtcgg tttgctcgac gccgatattt
    11101 acggcccgag cattcccacc atgctgggaa tcgccggtcg cccgatgagc aacggcgaga
    11161 agatcgagcc gctcgagcgc tacggtctca agctgatgag cattgggttc atgctcgatg
    11221 atcccaagtc cgccgtcgtg tggcgcgggc cgatgctgca cggcgccttg ctccagtttc
    11281 tgaaggatgt cgcgtggggc ccgctggact tcttgctcct ggatttgccg cccggtaccg
    11341 gcgacgtggc gctcacgctt tctcagaagg ttcgcgcatc gggcgccgtg gtggtcacca
    11401 ccccccaaga agttgcactg caggacgtct acaaatctgt atccatgtgc cagaaggtcg
    11461 gcattccaat cctgggtgtc gtggagaacc agagttactt cctgtgtgac ggctgcaata
    11521 aacgccacga gctgttcggc gccggcggcg gcgccaagat cgccgagtac gcggaggcgc
    11581 cgttgctcgg acagattccc ctgaaccctg ccgtccgaga gtggggagac gccggcacgc
    11641 ccattgtcca tgccgcaaaa ggatctccgg cagcccaggc gttcatggat gtcgcagcgc
    11701 ggctgctcga gaccatggcc gcccagggtg aagtagacgg tctggcgatc gatcgtagcg
    11761 ggggcaagaa ccgacacctg cccatcaccc gctaaatttc gggtgcagat tcatctgcat
    11821 aacatcagat cacgccacga gacccctccg cgccgaaccc cggatcgtgc tacgggaagc
    11881 cggggatttc ttctacccag atttctgatc gtcccagtcc ccgatcgaac cctgataacc
    11941 tcgagtctgt cggacgagcc tgttgggtag aaccctgcaa tcagcacacc cgtaaatcag
    12001 cacctcgtag aacagtaccg cgtcgaaaca ctgcactgaa aaggcgctgt agagacactg
    12061 caccttcgaa agactgcacc cctcgaaagt gcatcgccga aaccgctgag ccgattcacc
    12121 gcctcggaac gtccacctac ccgctagctt ccatcgcctc cccagattcc ctttcatagt
    12181 gaggcgcgat atacgctgga gcgtccgtca tgagtcacga acaatccgat acgcccgaca
    12241 ttgcacagag taacagcgca tcttccgaga gcgctgcggg cgagagcgct gcgggcaatg
    12301 ctgcggcaac cgtcaacact ccggctgaag gagcggccgc aagcagctcc gaaggcggtt
    12361 ccaccgaggc gcgggcagcc gaggcagccc ccgtggaagg cggggatgaa ggagaggacg
    12421 gcgaagagac ggacgcggaa ggcgcgagtg agggcgaggc cggtaaagaa gccgcgagtg
    12481 ccgacggggt ggccaagaag aagcgccgcc gcaagcgcaa gaagaagaag cccgaagggg
    12541 cggctgcaca cgcgccaggc tcggaaggtg aaccccggga gagtacgcgc ccgcatcgcg
    12601 ccagcgaacg cgcgccgttc cacgtggggg aagaggtgtt cggcaaggtc accgcagtga
    12661 tcgacacggc gatcatggtg gatctctccg gcaaggcgct ggcgatcttc gatcgtagcg
    12721 agatggagcc ggatgatctc gtccccgtgc cgggcgaacg cttcgttgca cgggtgcaca
    12781 acgacgggac ccgtggtggt ttggtggtcc tcacccgcaa gccgctacgc gaagaggaaa
    12841 ccaaggccaa gctcgaagcc gcgttcaaga acggggagct ggtttccggc ctgattacgg
    12901 gcgtgatcaa gggcggggtg gaggtcgaca tcggcgggat ccgcgcgttc gcgccggcgt
    12961 cgggtatgga tctccacccg ggccaagcca acttcacgac cctgctcggc caacgcatgg
    13021 acttcaagtt ggtgagcgtc gataagagcg gccgcgaagt ggtcgtgacc cgacggccca
    13081 tgctggaagc ggaagctcac gagcggcgca aacgcgcgtt gtcgctgctg accgaaggtc
    13141 aggtgctgcc gggcgtggtg cgcacggtgg tggagtgggg cgcgttcgtt gccctgcccg
    13201 aggcggagaa tctcgaaggc ctcgtgcacg tgtccgaggt gagccacgat ccgcgcgcgc
    13261 cgatcaccga gctcgtgaag cccggcgatc gcttcgaagt caagatcatc aagatcgacg
    13321 agaagggtaa gatttggctc agccgcaagg ctctagtcga tgatccttgg gcttcggctc
    13381 gccgtaagtt cgaggtccgc tcgcgtcaca ccggaaaggt cacgcggctg gagaagttcg
    13441 gtgctttcgt ggagctcgag cccggaatcg aagggctgct gcacgttgcc gacctgtcgc
    13501 tcgagcgcgt cgagcacccg aacctggttt tgaaagaagg ccaggatctc gaggtgctgg
    13561 tgcaccactt cgatccgcgt tccaagaaga tcaccctgca ccccgcccca cccgcggatc
    13621 gaccggaaga agctccgcag aagatcttgc gcaacgcgct gatcaaggcg gaggtggtga
    13681 agggcgaggc ggccggtgta gtcgtgcgcg tactcggggt tacggggcgc gctgcccggg
    13741 gcttcatccc cgcgggccaa accggtacca accgaggaac tgacctacgc aaagccttca
    13801 agcccggtag cgtcatcgac gtaaaggtgc tggacatcga cccgcgttcc ggcgagccga
    13861 agctcagcat ccgcggtttc aaagaggacg aggagcggcg cgcccacaac gagtaccgac
    13921 agaagctcaa ggaagagggc ggcttcggta cgttgggcga cctgctcaag aacaaactgc
    13981 gcgccgggga agagcgggaa gtctagagct gctctcgagt tgagctgctc taaagattga
    14041 caggagtggc tggaagttct ccagccactc ggccgacccc aaggcacgtg gaggttggtt
    14101 gtccaatagc gcagtggtga attatcgtgc tgcagtagaa agccagattc gccgggccac
    14161 ggacccgtcg ctgggatgtc ggaacagacg gatcgcgaac tcgtcgaggc agcacaaaac
    14221 ggagacgcgg aggcgtttgg ggtgctcatg cggcggcacc aacgccgggt gtaccgattg
    14281 gcactgcatc tcgtgcgcaa cgctgcggaa gccgaagacg tgacccagga aacgttcgtg
    14341 cgggcttatg gcgcgctcga ccgtttcgac ggtcgcagcg agcctttcac ttggatttac
    14401 cgcatcgcgg tgaacctctc tctgaacgcg attcgcgcca gaaagccaac ggatcgttcg
    14461 gcgccacccg acgatcctcg cgtggaaccg ctgctcgtgg aacggcgcgc caagctcgcg
    14521 agtccggagg ctgccagtga ggacaaggag ctcgcgcagg cgttgctcag cagcatcgat
    14581 cgtctgagcg atgtgctgcg cacgacgctg gtgctggtga cggtagacgg gttaccgcaa
    14641 gcggacgttg gccggattct cggctgcagt gaggggacgg tcgcgtggcg cgtccacgag
    14701 gcgcgccgca agctgcgtga atatatgtcc gagctcggac atggagagaa cgatggctga
    14761 gcgcagtcca agcttgggcg atcgcgcgga ggcgctgctt tcgagcttcg ggatgcccga
    14821 gttcgatgag gaggctctgg cggctcgcat ccttgcgcgc ctcgacccga gcgcccgagg
    14881 cagtacggac tcggcgttgt tgcaacctcc cctaccggac gatgctgggg agccgggagc
    14941 actcccgctc tcggtgcgcg ccgagctgct cgatttggag tcgcattcgc gcttgttacc
    15001 cgcggatgtg ttgcagccgg ttgtgcgagc tccggacgta gtttcggttc ccgcgctgtc
    15061 gcccaacgag ctctccccgg gtgccttgcc ggcgccgctg gtacccgcag gagtgggcag
    15121 cgggaacact ctcgcgagcg acggcgaacg cccgactgaa gtaacagagc taccggcggg
    15181 gctgtccctc tcggggtcat ccgagggcac atccgcagca ggcttgccgg gcgcaggctt
    15241 gccgggtgca ggtttgccgg gtgcagagtc tggtgcggca gcgctgctgc ggcccgcact
    15301 cggcggcttg acgccagccc ctcctccgtc cggtcagagc ttggccgcct tggcccgcgc
    15361 ggtcgcccag agcggcagct tagggaaggg cgcgcggagc caggtcacgg acatcgccaa
    15421 ggagagcctg tcggtcgcgg cggcggctcg tgcgcaaacc gatgccatcg tcgagcgcgt
    15481 tcgaagcgcg gaaaaagcac cggaaagcgt gcctccaccc gccacgccgg cgcgggtctt
    15541 acctgccacg cctgcttcgc tgaccaccgc cacgacggcc cgcagcgcgc ctccaccgcg
    15601 tacccgctca cctgggaatt ggcagtggct cgcgctggca ggcaccttgg ccctcgccgc
    15661 gggtgtagtg ttggtcctgc gcggctccga aaagtcggcg agcgcaccgc ccgcgcttgc
    15721 ggaaacgcgc gcgcaggcgg ccagcccgtc accgagttta ggcgcgggac cagcgccggt
    15781 cgaaggtgca ctcggggagc cgcacgccgc ctccgcatcc ccgagtgacg ccccggccgt
    15841 cgccgcgcat gaagcctcgg ctcctcaagc cgaggcccgc gccgcgctcg cgatcgctcc
    15901 acgcagtgcg gctaactcgg gctcggtcga acgcaccaat ccaggctttg gcgccgatgg
    15961 cgccgcgagc cccgagcgtc cgctggctcg cgcgccgagc agtgcgcgtc cagcccggcc
    16021 ccaacccgag tcgatcgttc tcgaagaaac gccgacggcc agccaacccg acgcgaactc
    16081 ggccaccaat cccgaggcgc atctcaagcc ggcagcgggc tcggccgcgg caggagggct
    16141 acccgacaag cccgcgaccg gcgcagtaca agctgccttg ggcagcgtca tgggcgcggc
    16201 ccgcgcctgt gtagcgggcg cggcgacccc aacccctgcg cagatcgtgt ttggatcgg
//
LOCUS       DASTKU010000309         5910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1399277, whole genome shotgun sequence.
ACCESSION   DASTKU010000309 DASTKU010000000
VERSION     DASTKU010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399277"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1648)
                     /gene="csx17"
                     /locus_tag="VFQ61_11085"
     CDS             complement(<1..1648)
                     /gene="csx17"
                     /locus_tag="VFQ61_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csx17"
                     /protein_id="HET9955042.1"
                     /translation="MSNAVRATHPIHEVELTGCRPEPLASYLKALGILRVVAQQKDPR
                     ARGCFRGEQFILQSALDRNALVSFFEIEYRPTPVIAPWNGGSGFYPKDNRKAADAIVS
                     SKDARLQEYAETIRIAREFVAAKGWTERPADENKQLMIASMRACLPDSALAWLDAAIV
                     LGNDRVLFPPLLGTGGNDGRLDFSNNFQQRVFEVLTKSPQTPALESSLFGVAQTSQFE
                     GAMGQYQPAANDRTNPWDFILLIEGALLFAAAATRRYENLGSAALAFPFHARAAGGLA
                     TVTEGDENESRDELWLPLWTSPASLPELRHLFAEGRATVGAGSNARSASTALDFARAV
                     SSLGVDRGIRSFVRVGFHVRNGLAYYATPLGRFATRQVPAARLLDDIDTWFDRLRRAA
                     GSNNCPARVSLAKRRTEQSLFEAAQTGRLSPVLLELGATERALAMSLGFTQKQFLQPV
                     PRLQSAWSQAVAENSAEQRLGAALAMRVGMRRRLIPLEASGWAFGRADQPDFVFSDRP
                     LVENLHALLLREQLEELQNASPKNRASVPDEICSLSDIAAF"
     gene            complement(1641..3998)
                     /gene="cas3"
                     /locus_tag="VFQ61_11090"
     CDS             complement(1641..3998)
                     /gene="cas3"
                     /locus_tag="VFQ61_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HET9955043.1"
                     /translation="MRFEEFFESATGRAPYSYQRTFATSESLPDLLEAPTGSGKTATA
                     VLGWMWRRLHGSESQRAEAGSRLIFCLPMRTLVEQTERVVLGYREKLGLSETLGVHVL
                     LGGAVDDRWEGFPDENAILIGTQDQLVSRALMRGYGMSRYLWPVHFALLNSDCTWVMD
                     EVQLMGVAASTAAQLQAFREGWSVAGHCKTVWMTATLSEERLRTVDNVRPFTRQPFEA
                     KEPALLKRLGARKALSSASLKVAKKTSLQALAREIAAAHEAGSLTLVVVNRVARAQEL
                     RALLAKEKGIESVALVHSRFRPAERRRIQEAALNGSFHGVLVATQAIEAGVDISAKTL
                     FTELAPWASLVQRFGRLNRAGEHAEAKAVWIDLDTTDPEVALPYETDELDAARLKLQS
                     LTDVGPASLAAVPKAPEPVALPVLRKRDLLELFDTEPDLAGRDIDVSRYIRATDDRDV
                     QVAWRELGKEPPGEDAPDLHRDELCSIPIWAFKRLAKDQSVWRFESLHGRWVKTEESR
                     LAPGMTLLVDVNVGGYDSELGFTGEKRDVPKALRLAARPPDDDESDRLTYGSSDYVTL
                     RMHSEDTAAEMQRLMDRLAPLLRGSVQTEELIRAARFHDLGKVHSDFQRMLLAALSDD
                     DPRRNGGPWAKSDGQSRTRNPRRFFRHELASALTWLAEGGSSLGAFLIAAHHGKVRLS
                     LRARPGEEPPSDVSGPVRFAHGVCDGDLLPQTDLGDGVIVPPQKLSLACMELGGGGEG
                     PSWADRMQQLLSELGPFRLAYLEMLIRIADWRASQKRAQGVNLDV"
     gene            complement(5643..>5910)
                     /locus_tag="VFQ61_11095"
     CDS             complement(5643..>5910)
                     /locus_tag="VFQ61_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HET9955044.1"
                     /translation="SSFETTTHVTRVQLRNLGSQPKAIEVVERIPVSEIEKVVIELDR
                     ELTRPAPTRFESGFVTWQIQLAAYSSLELQLTHIVRRHSDVHGI"
BASE COUNT         1129 a         1934 c         1735 g         1112 t
ORIGIN      
        1 tgaacgcagc gatgtcgctc aaactgcaga tttcgtccgg cacgctggcg cgattctttg
       61 gcgaggcatt ctgtaactct tcgagctgct cgcgaagcag gagcgcgtgg agattctcaa
      121 ccagagggcg atcgctgaat acgaagtccg gttgatcggc ccgcccaaaa gcccagcctg
      181 aagcctcgag cgggatcaag cgtctgcgca tgccgacgcg catagctagt gcagcgccga
      241 gcctctgctc cgcagaattc tctgcgaccg cttgggacca ggcgctttgc aagcgaggca
      301 ccggctgtag gaactgcttc tgagtgaagc cgagtgacat cgcgagcgcc cgctcggtgg
      361 ccccgagctc gagcaggaca ggagataagc ggcccgtctg cgccgcttcg aagagagact
      421 gttcagtgcg gcgcttggcg agagaaactc gcgctgggca gttgttgctg ccagcggcac
      481 ggcgtagccg gtcgaaccaa gtgtcgatat catcgaggag tcgtgctgcc ggaacttggc
      541 gggtcgcgaa gcggccgagt ggcgttgcgt agtaagcaag gccgttgcgg acatggaacc
      601 ccacccgcac gaagctgcgg atcccacggt cgacgcccaa cgagctcacg gcccgggcga
      661 agtcgagcgc ggtcgaggcg ctgcgcgcgt tcgagccggc gcccaccgtg gcgcggccct
      721 ctgcgaatag atgacggagc tcgggtaaac tcgcagggct cgtccaaagt ggtaaccaga
      781 gttcgtcacg gctctcattc tcatcccctt cggtgacggt ggctaagccg cccgctgcac
      841 gagcgtggaa tgggaaagcg agggccgctg agccgaggtt ctcgtaacgt cgggtggcgg
      901 ccgccgcaaa caacaaggca ccttcgatca gcaggatgaa atcccacgga ttggtgcggt
      961 cgttcgcggc cggctggtat tggcccatgg caccctcgaa ttgagaggtt tgggctacgc
     1021 cgaacaacga ggactctagg gctggagtct gcgggctctt cgtgaggact tcgaacacgc
     1081 gttgttggaa gttattgctg aagtccaaac gcccgtcgtt gccgcccgtg cccaggagcg
     1141 gagggaacag cacccggtca ttgccgagca cgatcgcggc atcgagccac gctagcgctg
     1201 aatcgggcag gcacgcccgc atgctcgcga tcatcagttg cttgttctcg tctgccgggc
     1261 gctccgtcca acccttcgcg gccacgaact cccgagcgat tcgaatcgtc tccgcgtact
     1321 cttggaggcg agcgtctttg ctgctcacga tggcgtccgc cgccttacga ttgtccttcg
     1381 gatagaaccc gcttcccccg ttccagggcg caatcaccgg cgtggggcga tactctatct
     1441 cgaagaagct caccagagca ttgcgatcga gggcgctctg caagatgaat tgttcgccgc
     1501 ggaaacagcc gcgcgctcgg ggatctttct gttgggcaac aacgcgcaaa atgcccaacg
     1561 ctttgagata cgaagcgagc ggctccggtc tgcaaccggt gagctcgacc tcgtgtatcg
     1621 gatgagtggc tcgcaccgca ttagacatcg aggttcaccc cctgcgcgcg tttctggctg
     1681 gctcgccaat cggcaatgcg gatcaacatc tcgagatacg ccagccggaa tgggccgagt
     1741 tcactcagca gctgttgcat ccgatccgcc caggaaggcc cttcgccacc cccgcccaat
     1801 tccatgcacg ccaacgagag cttttggggc ggcacgatca cgccatcgcc caagtcggtt
     1861 tggggcagca aatctccgtc gcagacgcca tgggcgaaac gcaccggccc actcacgtct
     1921 gacggaggct cttcacccgg acgcgcgcgc agcgagaggc gaaccttgcc gtgatgggcg
     1981 gcaatcagaa acgctcccaa cgacgaacct ccttcggcaa gccacgtcag tgcactcgcg
     2041 agctcgtggc gaaagaagcg ccggggattg cgggtgcgtg attgaccatc gctcttggcc
     2101 caagggcccc cgttgcggcg cggatcgtca tcggacaatg cggcgagcag catccgctgg
     2161 aaatcagagt ggaccttgcc tagatcgtgg aagcgagccg cacgaatcag ctcctctgtt
     2221 tgaacgctgc cccggagcaa aggcgcgagc cggtccatga gcctttgcat ctccgcggcg
     2281 gtgtcctcgg aatgcatccg caacgtgacg taatcgctcg agccataagt cagacgatcg
     2341 ctctcgtcgt cgtcgggcgg gcgcgcggcg agacgtagcg ctttgggtac atctcgcttc
     2401 tcgcccgtga agcccaactc ggagtcatag ccacccacat tgacgtcaac tagcaaggtc
     2461 atcccggggg caagccgtga ctcttcggtc ttgacccagc gaccgtgcag gctctcgaat
     2521 cgccacaccg attgatcttt ggccagccgt ttgaacgccc aaatcggaat cgagcacaac
     2581 tcatcacggt gcagatcggg cgcgtcttcg ccaggcggct ccttgccgag ctcgcgccac
     2641 gccacctgca cgtctcgatc gtccgtggcg cggatatagc gactcacgtc gatatcgcgc
     2701 ccggccagat ccggctctgt atcaaacagc tcgagcagat ctcgtttgcg caacaccggt
     2761 agggcgaccg gctccggcgc tttcgggacg gctgcgagcg aagccgggcc cacatccgtg
     2821 agcgattgca gtttgagcct cgccgcatcg agctcatctg tctcgtaggg cagggccact
     2881 tccggatcag ttgtatccag atcgatccaa acggccttcg cctccgcgtg ctcgccggcg
     2941 cgattcaggc gaccgaagcg ttgcaccagc gacgcccacg gggcgagctc cgtaaagagc
     3001 gtcttggccg agatatccac cccggcctct atggcctgtg ttgccacgag caccccatga
     3061 aacgagccat tgagcgccgc ctcctgaatg cgacgccgtt cggccgggcg aaagcgcgaa
     3121 tgcaccaacg ccaccgattc aatacccttc tccttagcga gcagagccct gagctcctgc
     3181 gcccgcgcca cgcgattgac caccaccagg gtcaaagacc cagcttcgtg cgcagcggca
     3241 atttcgcgtg cgagtgcctg aagcgacgtc ttcttcgcca ccttcagtga cgcgctggac
     3301 aatgccttac gcgcgcccaa ccgcttcaac aacgcgggtt ccttcgcctc gaacggctgc
     3361 cgagtgaacg gccgaacatt gtccaccgtg cgcaaacgtt cctcggagag cgtggccgtc
     3421 atccagaccg tcttacaatg cccggcgacg ctccagcctt cacgaaatgc ctgcaattgg
     3481 gctgcggttg aagccgcaac acccatcagc tgcacctcat ccatgaccca agtgcaatcg
     3541 ctattgagca gcgcgaagtg caccggccag agataccggc tcatgccgta gcctcgcatc
     3601 agcgcgcgag acaccagctg gtcttgtgtg ccaatcagaa tcgcgttctc atctggaaat
     3661 ccctcccatc ggtcatccac ggcgccgccg agcaacacat gcacaccgag cgtctctgac
     3721 aacccgagct tctcccggta accgagcacc acgcgctccg tttgctcgac cagtgtgcgc
     3781 atgggaaggc agaaaatcag cctacttcct gcctctgcgc gttgcgattc actgccgtgg
     3841 agcctccgcc acatccaccc caacacggca gtcgccgtct tgccggaccc cgtcggtgcc
     3901 tccagcaaat ccggcaagct ctcgctcgtg gcgaacgtgc gttgatacga gtagggcgcc
     3961 cgtcccgtgg ccgattcgaa gaactcttca aagcgcatgc ttcccaccgg gcgagcttca
     4021 ttcggagcgc tcgccttcta tgaccgatga ctttcggcga tccgtgaggg gcgcgtcaac
     4081 cccttcaagt aacaatcgag ttggttactg atcgatccga tcgcgtccca atcgaatccc
     4141 gtcagctttg tgacgccacc cgactgtctt tctcgtccgc cgctcgggtt tgccggtttt
     4201 tgcgctcgtt gacgcgccgc ctattagcct ttctcacaat ttccaacgtt ccctcgcgcg
     4261 accaggagtc accctgctga tgcgtacttg ccgaatgagc ctcggttcca tcgccaagta
     4321 ttgagtcgaa cgctcggttg ttagccgttg gactagtgtg ctcaattcct acctggtctt
     4381 cctcggcacc ggacacatcc cggtctgtca gacagtgctc caacaccacg ggttgggagg
     4441 ccatcgttgt tagtgcgcga ggagcacccc cttagatgac ggattcgttc ttcacactcg
     4501 ggccgtctgc gcggtgtcag ccggcttggt aaaaaatccc gttgaagaaa gtaccattga
     4561 cggttcgccg agaccgacgg cgtttcgcta tcctcgtcgg attgacggcg ctcgcgcaga
     4621 cggagagagc ttcagtccga gctctcgacg cacaccctca tccaaggcgg cagagccact
     4681 tcaccgctct cgttcggcgg cgtcgcgcac cggaccgtac tgatgcattg gcttgatttg
     4741 gaacggcaga gttgccgtgc atagggctcg caatcgacat cggtattgca gagcatcatc
     4801 gtagcgtcca agttgcacgt gttggcgcaa acggtggcct gggcaccgat tgaatcactc
     4861 gtgcagcact tctggcttcc acaatcgttg ggttgggtgc attggtaaac ggcctggaaa
     4921 cttgccgggc tccccgccac gagcttctcc gagagacgtt cggcctcttg aactcccata
     4981 catcgcgggg aatcgctcca tgcgcaatat gggctctctg gggggcagct ttggccggga
     5041 gagcacgtcg ccagcgcgtc gacgcggcaa tacccgtccg agcaccgctg tcctcccggg
     5101 caaagcactc cgtctggccc ggagcacgcg atagccgcac agttgtcatt cacacgctca
     5161 cacgaatgaa cggtgtttcc cgacgcgaaa attcgacagc aggccaggcc accagagcag
     5221 tcactcgaat catcgcacgc ataccggccg tcgtaatcac cccgcgcggg gcggcaggcc
     5281 cagtccgccc cattgtctac ttgcgtgcaa acctcttcac ccgctctgca gcgagcacgt
     5341 ccgcacgcaa tcgtcttcat cgtagatcct ttcgcgctgt cccgagggac gaacctcgca
     5401 gcgggcgatg ccgcaaaaag ggcgtgccga ccacgagggc cgtcctcgga cattggagtt
     5461 ctgcaaccgc tcaagatcgc caagacgagc agcgccctca ctagctccat actccgctgc
     5521 gtgagtttgc gggacccatc gtaaatcaac gatgaccaga gccctgcgcg cggacccttc
     5581 cccgttctct cgcgagccgc cacctccacg ctgcgcatgc gggtcgtcgc gctccggctg
     5641 actcaaatgc cgtgcacgtc ggagtggcgc ctcacgatat gcgtgagctg gagctccaat
     5701 gaagagtaag cggctagctg gatctgccag gtcacgaagc ccgattcgaa gcgtgtaggc
     5761 gccgggcgcg tgagctcccg atccagctca atcaccactt tctcgatctc agacacgggg
     5821 atgcgttcga ccacctcgat ggccttcggt tgcgagccca gattacgcag ctgaacgcga
     5881 gtgacgtggg tggtggtctc gaaggaactc
//
LOCUS       DASTKU010000310         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_282024, whole genome shotgun sequence.
ACCESSION   DASTKU010000310 DASTKU010000000
VERSION     DASTKU010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282024"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1317)
                     /locus_tag="VFQ61_11100"
     CDS             complement(211..1317)
                     /locus_tag="VFQ61_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955045.1"
                     /translation="MLNRSVFVCFPLLFGACSGTSTGDSQGTGGTTASGGSTASGGST
                     ASGGSGISANGGATATGGAVTSGGASMTSGGVAASGGSQSSASGGVSAAGSAATSGGK
                     SAGGDAGATSASGGSGGSSSGACTRSDLQATVNAYLKALVAHDATMAPLASSARYTEN
                     TKEVKVGEGLWKTAGEVKLQRDLLDTVQCSTVSQVVLPESGADIVFTQRLKVEGGKIT
                     ESEAVITRKGDWNFDATGYLNTKSKTWDVLPADQRTPRDTLISMPKVYWDYFKENKPP
                     MPPFGDGCKRVEGGKGAPINCTVGIPSGVTISDRRFWADEEAGVSAGISIFGGTSGLL
                     DVHYFKMVSGTIRDVYAMTVNTSYMGTGWPGGVP"
BASE COUNT          411 a          739 c          755 g          474 t
ORIGIN      
        1 gctgcgaagg ccgatttctc tgcgggctct tcgatctcaa agcgcgacgt ctcatcgcgc
       61 cgacacccga gtggctggcc gcggtcggtt gtccgccgag ctcttagagg gccgcgaatg
      121 gatcggcgta catcagcgcg aggggatcat ctttctccga cgcgcccgct cggatgagca
      181 gtcttcaggt gctttgcggc tcgggtccgt tcacggaacg ccgcccggcc accccgtgcc
      241 catgtagctc gtgtttacgg tcatggcgta gacgtcgcgg atagtcccac tcaccatctt
      301 gaagtagtgc acgtcgagca ggcctgaagt ccctccgaag attgaaatac ccgcggagac
      361 gcccgcttct tcgtcggccc aaaagcgccg atccgagatc gtaactccgc tcggaatacc
      421 gacggtgcag ttgatcggcg cgcccttgcc gccctcgacc cgcttgcaac catcgccgaa
      481 cggcggcatc ggcggcttgt tctccttgaa gtagtcccaa tacaccttgg gcatgctgat
      541 cagcgtgtcg cgcggcgtgc gctggtctgc gggcaggacg tcccaggtct tcgacttcgt
      601 gttgagatac ccggttgcat cgaagttcca gtcgcccttt cgggtaatga cggcttccga
      661 ctcggtgatt ttaccgcctt cgaccttcaa tcgttgggtg aacacgatgt cggcaccact
      721 ctcgggcaac accacctgcg acacggtgga gcactgaacc gtgtcgagca gatcccgttg
      781 cagcttcact tcgccggcgg tcttccaaag cccctcgccg actttgacct ccttcgtgtt
      841 ctctgtatag cgcgcgctgc tcgcgagcgg cgccatcgtg gcgtcatggg cgacgagcgc
      901 cttgaggtag gcgttcacgg ttgcttggag gtcacttcgc gtgcacgcac cgctgctgct
      961 gccgcccgag ccgccactcg ctgaggttgc accggcatct ccgcccgcgc tcttgccgcc
     1021 gctcgttgca gcgctgcccg ccgcgctcac tccgccagat gcggaactct gggagccgcc
     1081 gctcgcggcg acaccaccgc tcgtcatgct ggcgccgccg ctcgtaacgg cccctcccgt
     1141 tgcggtagcg cctccatttg cgctgatacc agacccaccg ctcgcagtag agccgccgct
     1201 cgcagtagag ccgcccgatg cggttgtgcc tccggtcccc tgactgtcac cagtgctcgt
     1261 gccggagcac gcaccgaaca agagcggaaa acagacgaac accgagcggt tcagcatgac
     1321 tcctccacga gaggtgcgtt cgtgagtcaa ctagcagccg ctcccaagcg attcaagctc
     1381 gcgcgcgaac tcgtgtcgtg tcgttcgagc ggagtttcca tgctttccga gcacagaaga
     1441 tcttcgcgct cgagacatac gcgcgcgcaa gccgcagagt agccgcctgc ggtcggtgga
     1501 gtgcgcagct gcttccgcgc gaacatacgg gattgcgaaa acggcacatg ccgagtggcg
     1561 gaattggcga gagaagattg gggcgttgcc gtaactctcg cggtcagctg gcacaatcat
     1621 gaaagaatgc tctgtgagcg cgagagtatt tcagcgcgga gccgatcgcg aagccgattg
     1681 gcgggcatgg ggttccgcga ttcgactcag gtcctgaagc tccgctgctg ccgcgtgcct
     1741 tcgggcttcg tgaccttggt ggagaagcgc tgggtggggc gggcgccctt ctggtggcaa
     1801 gccggggcgt ttgcaacggt tcggccgcgt cggttcacga ttgcatcgcg cgtcggggct
     1861 ttgcaacatc gttgcccgga acgagcatga cgtttcgggc gcggtgatgc cgcgctcgca
     1921 atgcgcggag gtagattgca ggaatggtga ggagccgttc ggctcgagtc agaaagacat
     1981 tgagattgag aactcgattg gccgtgagcg cgagaggcga atagggaagg acttcgttgc
     2041 gagcttgggt gtgtagttct cgagccaagc cctccgagtc gctctaaatg cactggatgg
     2101 agtcaccgta cgagagcggg cgcgagcggt tctgccaggc gattgcgcca gtctctacgc
     2161 atcccttgcg taatctcgac ccagctgcgc agatctttca tctgcacgag aactcaagaa
     2221 actgcgctgg gtgccgctag caggtatgtg gtcgaggcgc gccgagatgg gccaccgtcg
     2281 agcgccgatt ctcgagagat gcgtatgttt ggttggcgcg agagctacaa ggtcgacatt
     2341 cgaaggatcg acgaacagca tcgagagctg ttcgcgctg
//
LOCUS       DASTKU010000311        13409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_728743, whole genome shotgun sequence.
ACCESSION   DASTKU010000311 DASTKU010000000
VERSION     DASTKU010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13409
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728743"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1670)
                     /locus_tag="VFQ61_11105"
     CDS             complement(<1..1670)
                     /locus_tag="VFQ61_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET9955046.1"
                     /translation="MDRVAAVESLARCVALGDDVDGALSRLEGQFRGTNEEPDAARLA
                     SAVARIYPLLPEHPQLQSSALDPLLESLEQLARKKAVDVARVLDSVALALASLDEAAK
                     PVVDEGNPAAHHEPNWSGKIAEDDLVLLPEFVLEAREQLGSAEQQMLFLEKVPGDSNA
                     LNTAFRGLHTVKGVAACLGITPIAELSHELETLLDRARQNVPLDQNRMADVVMRGIDR
                     LRNLVEWLAPVTAGGNPDGEWPKVGDLLEEARGVVSATARIDASQTAAVAALEAVVSK
                     PPAKAPESQVSPSIPGSVKPEPPASVMPNSMMGFSPTVAPTRSFTNSTLDQLTSDLRS
                     PGSPAASSLGHPPQGVGLEGERAAPAPPRPAPGPLPTWAPGANRRSRAPETMMPGNQV
                     AAASTVKVDTRKLDALMDLVGELVVTQSMVAQDPDLASLSSQRLTQSLALLTRTCKEL
                     RRVAMSTRMVPIAATFDKMARVLRDVSRALNKPAELVVEGADTELDRNVVETLHDPLL
                     HMIRNSIDHGLETSADRLRLGKPHAGRVTLRAYHEGGQIVVEVSDDGKG"
     gene            complement(1857..2948)
                     /gene="hisC"
                     /locus_tag="VFQ61_11110"
     CDS             complement(1857..2948)
                     /gene="hisC"
                     /locus_tag="VFQ61_11110"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET9955047.1"
                     /translation="MSKFWSPRVRSLSPYVPGEQPKLEQLTKLNTNENPYGPSPRVRA
                     AVEAALGDSLRLYPDPNSEALCACIARYYGVSPEQVFVGNGSDEVLGFTFLTFFDQAE
                     PILFPDVTYSFYPVYCNLYGLRYEALPLGPKFELDLGAYRRKNGGIVFANPNAPTSRA
                     VSRAEIAQQLLAHNTDSVVVVDEAYVDFGAESVIPLCREYPNLLVVQTLSKSRSLAGL
                     RVGFAIGDAELIAGLNRVKNSFNSYPLDRLAGAAAIAAFEDEAHYQSTRARVIDSREW
                     LTRELERLGFEVLPSAANFVFARHPAWTGSVLMRELRQRAVIVRHFDKPRISDFLRIS
                     VGTDAECQRLVRCLTEILGSSSDRTREQL"
     gene            complement(3045..3398)
                     /locus_tag="VFQ61_11115"
     CDS             complement(3045..3398)
                     /locus_tag="VFQ61_11115"
                     /inference="COORDINATES: protein motif:HMM:NF016427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 domain-containing protein"
                     /protein_id="HET9955048.1"
                     /translation="MASSEPLKPALAQVARSLLAEASHVLSLDVIAERLATLAVDTDE
                     IEQLFDYLEAHGKTVGDPGVGAASETLGAVLTAARSLKRELGRAPNSREIAEHAALPI
                     DAVRRALLFAKIVQR"
     gene            complement(3514..4185)
                     /locus_tag="VFQ61_11120"
     CDS             complement(3514..4185)
                     /locus_tag="VFQ61_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955049.1"
                     /translation="MAFGSVFSICVAACNGGEHVLGRDCPELIDDMEDGNPSIISKGR
                     VGEWFSYNDRSDGSIQRPPASAEVFPMETLSPPRGSSLRAVHTSGSGFTGVWGAGVGF
                     DLQLKNAYDASKYRGISFLARAGEDADLLGRTLRVNVPDGNTAAAGGRCEGEQCNDDF
                     GKEVQLGNEFIQYELDFDELTQAGWSGKLFEKLTLKELYGIRFQSQPNSKFDFWIDDV
                     AFTCR"
     gene            complement(4251..5276)
                     /locus_tag="VFQ61_11125"
     CDS             complement(4251..5276)
                     /locus_tag="VFQ61_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955050.1"
                     /translation="MTQFRRLIEMGDEELACSLIAAMQEEMPPAGSLARAGAAVGLPA
                     ALIAAPASLLAAPGAVSGVVVSGVGAGLAAGAGLGTPASVAVAGAAAPVVAASASALP
                     GGLLPATLSALFKPFLMGLLGGAFALGGVQLALKTAPRAVEARSMEAGSEASDARGLA
                     RSAGERNGSNAAALESPPPIGALASPRGGDSAIATDPSDGGAARAAQRPISASAQPRR
                     TDSGRAEFGSGSKPVGARATTTSLDNGEVLAITPSVTAALPDAPPEPSAAPEVAKKPG
                     PSALVAEIALIDQARTALLTGKPERVLAVLALYRGTSFLPEAEKLRSQAEEQLRKRKM
                     AETQMAP"
     gene            complement(5273..6010)
                     /locus_tag="VFQ61_11130"
     CDS             complement(5273..6010)
                     /locus_tag="VFQ61_11130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9955051.1"
                     /translation="MTLTYGIGAYASRGSTSASRQPSPLESREPLADRERDAPASEPP
                     TALEPPTRSGVIHNPEPFRPPMDASQRDAWLAELLRLHHACVWRTLRRLGVSEDRADD
                     AAQEVFIVASRKLDQIQPGRERNFLLSSALRVAANYRRSSRSRREVHDEELLSEQRDP
                     GPDAAHALDQKRLRQLVDGLMESWPEELRTSFVLFEMEGLSVPEIAELTEAKTGTVAS
                     RLRRARELFLAGAKRLRARGLLDGSRL"
     gene            complement(6044..7171)
                     /locus_tag="VFQ61_11135"
     CDS             complement(6044..7171)
                     /locus_tag="VFQ61_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955052.1"
                     /translation="MLSTAVWMGLTGAGAAMPSASAQESGHLPGEPLGHASGNQGSGK
                     QESGQGAPAAQPVYPFRVVFSAPAGCGSAEFFSRQVRRRTRRLEPAKRDQRAVTFFLY
                     LVRSEGAPGEDARGEGAPARGEASVRGQLGVGQPDGTLSFREVPGVNCNEVVDAMALI
                     AALTFDLAANTEALPPMPAEPERESPEEAPDSVSGHRGTELGFSFGTRAGVRSAVSPD
                     LALNVSVEAALTFLGGEIQPGIRLSGHAARGPKVQAAAGDAQFTLLAARIAGCPTRWA
                     LRSNWYATPCAWAELGVLNGSGSRIVVHNTERVLWGAAGLALHSELQIVGPLWLAAEL
                     SGFFPFRHDVFRFEPKIPDNELFRVPSTGALAALGLGLHFF"
     gene            7514..8506
                     /locus_tag="VFQ61_11140"
     CDS             7514..8506
                     /locus_tag="VFQ61_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955053.1"
                     /translation="MDLDLEIAHEGVPPGAPTTEARTEEARVAPVKTAGAHALRGPRA
                     FEAEPSRQLNDANSTQPNQSGEFPVPNAQTNPRGAELTPSNEGTERRESRPGLSAEQL
                     GIAGTNPFVTIPSRPTETPAGRARREQRELSARVERSLTDVISAQDQKRGLGPEGAVL
                     RTLEAEVLASNTAPNSSAVFDAMTDTAGYLVGLQLVEANSDSLFWHRLAESTLSRLGK
                     QRLRGSHGKNGLTLRIRVTSRIALPSGRDPGLAIDAFGLPIKKGAGKRSSRLSLFDVK
                     PQLKSTETTLPDGRVISTPTLQLFSLGRLEADPADIGAQPRRMVHAHVERSWQN"
     gene            9042..9164
                     /locus_tag="VFQ61_11145"
     CDS             9042..9164
                     /locus_tag="VFQ61_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955054.1"
                     /translation="MSIEWLALIASPVKVSRRVDVTGTKTNVTHTVNVTEDQQP"
     gene            10009..10374
                     /locus_tag="VFQ61_11150"
     CDS             10009..10374
                     /locus_tag="VFQ61_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955055.1"
                     /translation="MNTRVNRNGNHAGTSANPRGANLVKTRKPKRRGVPEPERRAVAA
                     EIRALLQAEDETGRRLWTQTSLGERCGGVSQQMIYLAQEPDGVGPSVRDGLLDFTGMT
                     MHQLLEKHGQLGRGGGGRS"
     gene            10423..10728
                     /locus_tag="VFQ61_11155"
     CDS             10423..10728
                     /locus_tag="VFQ61_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955056.1"
                     /translation="MDRHTAGPGKGGDALDPLAPFRRAMEALCDDGFPKAAVQKIVAE
                     LAFGSQVGRGGAAVPQRDALDIYRNARSRLEQGGDGKPKSGRAVARASGVQVSRRER"
     gene            10810..11898
                     /locus_tag="VFQ61_11160"
     CDS             10810..11898
                     /locus_tag="VFQ61_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HET9955057.1"
                     /translation="MSASSSVQHLVEPFSLGKSQLANRVVMSPMTRCRAIGNLPNALM
                     AEYYSQRAEAGLIVTEGTSPSPNGLGYARIPGCFDAAQVAGWRLVTSAVHDAGGKIFV
                     QLMHTGRVSNALNMPPGAQVVAPSAVALTGSIWTDSAQQQPYAVPRAMLLAEVREAIV
                     EYTRSARLAIEAGFDGVELHGANGYLIDQFFNTATNQRTDEYGGSVENRIRFAVEVAR
                     SVGEAIGFDRVGMRLSPYGTFNDMRPDPQMDEMYERLALELHRLGLVYLHVINHGDPS
                     SPEVRQSVRRAIRNTFKGALILAGRYDVAGAERELAEGNADLIGFGGAFLSNPRLVSK
                     IRAGSPFIPSNPATYYTPGAEGYTDYPV"
     gene            complement(12055..12672)
                     /locus_tag="VFQ61_11165"
     CDS             complement(12055..12672)
                     /locus_tag="VFQ61_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9955058.1"
                     /translation="MVLSLEAPKARGPAGKTPSNNLLNQPKAPADSLARAQVAYWYRT
                     ELPRVMRLVRRLGVASADVEDVAHEVFIVLQSRFTRLDRDAGLHFWLRAVTFRVCSNY
                     RRRAARRRSESLAEVERGAAAGLLEHHSPRPDECYLSNERRELLHRALARLDPCKREV
                     FLLSEMNDCTALEIATRMQVSPNTVASRLRAARRQIAQRLRGVRA"
     gene            13110..>13409
                     /locus_tag="VFQ61_11170"
     CDS             13110..>13409
                     /locus_tag="VFQ61_11170"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HET9955059.1"
                     /translation="MLNQSTESELNSLLEAEQHNAAIGLALRCYGPEVFSYLIVLLRD
                     KGLAEEAYSMFTEALVRGIRSFKQQCPFRAWAYIVAKNEALRVMRQRRSLLPLGST"
BASE COUNT         2555 a         4354 c         4135 g         2365 t
ORIGIN      
        1 cccttcccgt cgtccgacac ctccaccacg atctggcccc cttcgtggta ggcgcgcagc
       61 gtgacgcgac cagcgtgcgg cttaccgaga cgcaaccgat cggcgctcgt ttccaggccg
      121 tgatcgatcg agtttcgaat catgtgcagc agagggtcgt ggagcgtctc gacgacgttg
      181 cgatcgagct ccgtgtccgc cccttccacc accagctcgg cgggtttatt gagtgcacgc
      241 gagacgtccc gcagcacccg cgccattttg tcgaaggtag ccgcgatcgg caccatgcgc
      301 gtggacatcg cgacccgtcg cagctccttg caggtgcgcg tgagcagggc gaggctctgg
      361 gtcaggcgtt gtgacgagag gctcgccaga tctggatcct gcgcgaccat gctctgcgtc
      421 accacgagct cacccaccaa atccatcagc gcgtcgagct tacgggtatc gactttgacc
      481 gtagatgcgg ccgccacttg gttgccgggc atcatcgtct cgggcgcgcg cgatcgacga
      541 ttggcgccag gcgcccaggt gggcagtggg cctggagcgg gacggggcgg agcaggcgcc
      601 gcgcgctccc cctcgagccc cacgccttgc ggcgggtgcc ccaagctact cgccgcgggt
      661 gagcccggcg agcggaggtc agacgtgagt tgatccaacg tcgagttggt gaacgagcgc
      721 gtcggcgcga cggtgggaga gaaccccatc atcgagttcg gcatcaccga cgcgggcggc
      781 tccggtttga ccgatcccgg aatcgagggc gagacctgcg attctggcgc cttcgccggc
      841 ggtttcgaga ccaccgcctc gagggcggcg acggcggcgg tttgtgaagc gtcaatccgt
      901 gccgtggcgg ataccacgcc tcgggcttcc tcgagcaaat ccccgacctt gggccattcc
      961 ccgtctgggt ttccccctgc ggtgacgggc gccaaccact cgacgagatt gcgaaggcga
     1021 tcgatgccgc gcatgaccac gtcggccatg cggttttgat cgagcggcac attctggcga
     1081 gcacgatcca ggagcgtctc gagctcgtgc gagagctcgg caatcggcgt aatcccgaga
     1141 caggcggcga cgccttttac ggtgtgcagc ccgcgaaacg cggtattcag agcgttgctg
     1201 tcgcccggaa ccttctccaa gaacagcatc tgctgctcgg ctgatcccag ctgctcgcgt
     1261 gcttccaaca cgaactccgg caacagcacc aagtcgtcct cggcgatctt tccggaccag
     1321 ttcggctcat ggtgcgcggc cgggttacct tcgtccacga ctggcttggc cgcttcatcg
     1381 agcgaggcca gcgcaagcgc aacgctatcg agcacccttg caacatccac ggccttcttg
     1441 cgcgccagct gttcgagcga ctcgagcagc ggatcgagcg cgctgctctg cagctgcgga
     1501 tgctccggaa gcagcgggta gattcgggcc accgccgaag ccaagcgagc cgcatccggc
     1561 tcctcgttgg tgcccctgaa ttgcccctcc agcctagaca acgcgccatc gacgtcgtca
     1621 ccgagtgcga cgcatcgagc taagctttcg actgcagcaa cgcgatccat ccggcgaaca
     1681 ggacggcatc atggctgggc aattaaggga tttccgccac accccacgtc cacctatcgg
     1741 gcgcggggca ccgcgcaggc tccacccgca gccgagcgtg aggagcagcc acatgtcgac
     1801 gcattcgctc cgacacgcgc cgcatgagca caatcactca gcttgcgatt gggacttcag
     1861 agctgctctc gggtgcggtc cgagctcgac cccaagatct cggtcaggca gcgtaccagt
     1921 ctttggcact ccgcgtccgt acccacactg atgcgcagga aatccgagat tctcggcttg
     1981 tcgaagtgtc gcacgatgac cgcgcgctgg cgcagctcac gcatcaagac ggaacccgtc
     2041 cacgcggggt ggcgagcgaa aacgaaattc gccgccgacg gcagcacctc gaatcccaag
     2101 cgctcgagtt cacgtgtaag ccattcgcgg ctgtcgatga cccgcgctcg agtcgattga
     2161 tagtgggctt cgtcttcgaa ggccgcgatc gccgccgcgc ccgccaaacg atccaaagga
     2221 taggagttga agctattttt cacgcggttc aagcccgcga ttaactccgc gtcaccgatc
     2281 gcgaacccca cccgcagccc agcgagggag cgggatttcg agagcgtctg caccacgagc
     2341 aggttcgggt actcgcggca gagcgggatc acggactcgg ccccgaagtc cacgtaggct
     2401 tcgtcgacca cgaccaccga gtcggtgttg tgcgccaaca actgctgcgc aatctcggct
     2461 cgactcaccg cacgactcgt aggcgcgttg gggttcgcga acacgattcc gccgtttttc
     2521 cggcgatatg cgccgaggtc gagctcgaac ttcggaccga gcggcaaagc ctcgtatcgt
     2581 aagccgtaca aattgcagta caccggataa aagctgtacg ttacatcggg aaacaggatg
     2641 ggctcggcct gatcgaagaa agtcaggaac gtgaagccca gcacttcgtc cgagccgttt
     2701 ccgacgaaga cctgctcggg tgacaccccg taatatcggg caatgcacgc gcacaaggcc
     2761 tcgctattcg ggtccgggta gagccgcaga gagtccccca aggcggcttc gacagcagcg
     2821 cgcacgcgcg gcgacggccc atatggattc tcgttcgtgt tcagtttggt gagctgctcg
     2881 agcttcggtt gctcaccggg aacgtaaggc gaaagagaac gaacacgcgg gctccagaac
     2941 ttgctcatgg ctgctcgcgc aggtttccca gactccggtg cacaaatcaa cggagatggc
     3001 gcgtatcccc gagcgggcgc ttcactcggg gcctggtcga actctcagcg ttgaacgatt
     3061 ttcgcgaaca acagagctcg ccggaccgcg tcgatgggca gggccgcgtg ctcggcgatc
     3121 tcgcgagaat tcggggctcg gccgagttct cgtttcaagc ttcgcgccgc cgtcaacacc
     3181 gcccccaggg tctcggatgc tgcacctacg ccggggtcac cgacggtctt accgtgagcc
     3241 tccagatagt cgaacagctg ctcgatttca tcggtatcca ccgcgagcgt cgccaggcgt
     3301 tcggcgatca cgtcgagcga caacacatgg gaagcttcgg ccagcagcga tctcgcgact
     3361 tgagcgagcg ctggtttgag gggttccgac gaggccatgg ctggtgctag tgctagcagc
     3421 tcgacgggcg tcgaactacc atcccatagc ttcgagagcg tgcacgaggc gtcgcgcgcg
     3481 cccagccgcc gcgagcatta gcggcggagt cgctcatcga cacgtgaacg cgacgtcgtc
     3541 gatccagaag tcgaacttgg agttgggctg actctggaag cgaatgccgt agagctcttt
     3601 caaggtgagc ttttcgaaaa gcttacccga ccaccccgct tgagtgagct cgtcgaagtc
     3661 gagttcgtac tgaatgaact cgttccccaa ctgcacttcc ttaccgaagt catcgttgca
     3721 ctgctctccc tcgcagcgcc cgcctgcggc ggcggtgttg ccgtcgggca cgttgacgcg
     3781 cagcgtgcgg ccgagcaggt cggcatcttc gcccgcccgt gccaggaagc tgatccctcg
     3841 gtacttggag gcgtcgtacg cgtttttcag ctggagatcg aagcccacgc ccgcccccca
     3901 cactccggta aaaccagaac cggaagtatg aacggcacgc aggctggatc cgcggggcgg
     3961 agatagcgtc tccatcggga acacttccgc ggatgcggga ggtctctgaa tactcccatc
     4021 gctgcggtcg ttgtacgaaa accactcgcc gacgcgccct ttggaaatga tggaggggtt
     4081 tccatcctcc atatcgtcga tcagctctgg gcagtcacga cccagcacgt gctccccgcc
     4141 gttgcaagcc gcaacgcaga tcgagaacac ggagccgaac gccaaagcaa ccgctcgctc
     4201 aaccgacggg gcgaaccacc gatcgtggcc tcgatgcatg atggctgaac ctacggcgcc
     4261 atttgcgttt cggccatctt ccgctttcgc agctgttcct cggcctgaga acgcaacttc
     4321 tcggcctcgg gcaggaagga cgtcccgcga tatagtgcga gcaccgccaa gacgcgctcg
     4381 ggcttgcccg tcagcaaggc cgtgcgtgct tgatcaatca aagcaatctc tgccaccaag
     4441 gcggagggac caggcttctt cgcgacctcc ggcgccgcac tcggctccgg cggcgcgtcc
     4501 ggcagcgccg ccgtgacgct cggggtgatc gcgagaactt cgccgttgtc cagcgaagtc
     4561 gtcgttgcgc gggcgccgac gggcttgctg ccagagccga actccgctcg cccggaatcg
     4621 gtgcgcctcg gctgagcaga agcagagatc ggacgctgag ctgcacgagc ggcgcccccg
     4681 tcactgggat cggttgcaat cgcggaatcg ccgccgcgag gcgaagccaa tgctccgatt
     4741 ggaggcgggc tctccagcgc agcggcgttc gacccatttc gctcacccgc gctccgcgcc
     4801 agaccgcgtg catcgctggc ttccgatccg gcttccatag aacgcgcttc gacggcccga
     4861 ggcgccgttt tgagcgcgag ctgcacgcct ccgagcgcaa acgctccgcc cagcaatccc
     4921 atcagaaatg gcttgaacag ggcgctgagg gtcgcgggga gcaacccacc gggaagagca
     4981 gaagcagaag ccgccactac tggcgccgcc gcgccggcca cggccacgct cgcaggagta
     5041 ccgagcccgg cacccgccgc caagcccgcg cccacgcccg aaaccaccac acctgaaact
     5101 gcaccgggtg ccgccaacaa tgacgctggc gcggcgatca gcgcggcggg caaacccact
     5161 gcagccccgg ctcgcgcgag agagccggct ggcggcatct cctcctgcat agcggcgatg
     5221 agtgagcagg ctagctcctc atctcccatc tcgattaaac gtcggaattg agtcacaggc
     5281 ggctcccgtc cagcaagccg cgcgcgcgca aacgcttggc tccagcaagg aaaagctcgc
     5341 gagctcggcg caaacgcgac gccaccgtgc ccgtctttgc ttcggtcagc tcagcgattt
     5401 ctggaacact gagcccttcc atctcgaaca gcacgaatga cgtgcgtagc tcctcgggcc
     5461 aactctccat caagccgtcg accagctgac gcaaccgctt ctgatcgagg gcgtgcgccg
     5521 cgtcgggtcc gggatcgcgc tgctccgaca agagctcctc atcatgcact tcacggcgag
     5581 agcggctcga ccggcgatag ttggccgcca cgcgcagcgc ggagctcaac aggaagttcc
     5641 gctcgcgacc cggctggatt tgatccagct tgcgcgacgc cacgatgaag acctcctgag
     5701 ccgcatcatc tgcccggtct tcagagaccc cgagtcggcg cagtgtgcgc cacacgcagg
     5761 cgtgatgcag cctcagcagc tcggccagcc acgcatcacg ctggctcgca tccatgggcg
     5821 ggcgaaacgg ttcggggttg tgaatcactc cactgcgagt cggaggttct agcgcggtcg
     5881 gaggttcgct ggcgggcgca tcgcgctctc gatcggcgag cggctcccga ctctcgagcg
     5941 gggaaggctg gcgagaagcc gacgtcgagc cgcgcgacgc gtaagcaccg atgccgtaag
     6001 taagagtcac cggtgggagt atgtgcccag attgaagggc cgatcagaaa aagtgcagcc
     6061 ccaatccgag cgcagcgagc gccccagtgg agggtactcg gaacagctca ttgtctggaa
     6121 tttttggctc aaaccgaaat acatcgtgcc gaaacgggaa gaagccgctg agctcagccg
     6181 cgagccagag cggaccgacg atctgcaatt cgctgtgcaa agccaagccc gcggcgcccc
     6241 acaaaacacg ctccgtattg tgcacgacaa tgcgagaccc agagccgttc agcaccccca
     6301 gctccgccca ggcgcaaggc gtggcgtacc aattcgagcg cagcgcccag cgggtggggc
     6361 agccggcaat gcgagcggcg agcagggtga actgcgcgtc cccggcagcg gcctgaacct
     6421 tgggcccacg tgccgcgtgt ccggacaacc tgatccccgg ctgaatttca ccgcccagaa
     6481 acgtgagtgc ggcctccacg ctgacgttca aggctaggtc cgggctcacc gcgctacgaa
     6541 cgcccgcacg cgtcccaaaa ctgaaaccca attccgttcc gcggtgaccc gagaccgagt
     6601 ctggagcttc ctctggggac tcgcgttctg gttcggccgg cattggcggc aacgcctcgg
     6661 tgttggccgc gagatcgaac gtcagcgccg cgatcaatgc catggcatcg accacctcgt
     6721 tacagttgac gcccggaact tcccgaaaag atagcgtccc atcgggctgc cctaccccca
     6781 actgaccacg cacggacgcc tcgccgcgtg cgggcgcccc ctcgcctcgc gcgtcctcgc
     6841 ccggtgcccc ctcgcttcgt accagataca aaaagaacgt taccgcgcgc tgatcgcgct
     6901 tcgcgggctc gagacgccgc gtgcgtcgtc gcacctgccg agagaagaat tccgcgctcc
     6961 cgcagccggc tggagcagag aacacgacgc gaaacggata caccggctgg gccgccgggg
     7021 cgccctgacc cgattcctgc ttacccgagc cttgatttcc cgatgcgtgg ccgaggggct
     7081 cgccaggaag gtgaccggac tcttgcgcgg aagcactggg catcgccgcg ccggcgccgg
     7141 taaggcccat ccagacagcg gtcgaaagca cggctacggg ccttaggaca cggcgaagcc
     7201 aagtgttcaa ccagcccctc cgcgatggac aaactgagca ctcggcacgg aaacccacca
     7261 gtaccttgaa cctgcacgtt cgtctaccgc ccgtgcggtg gtgccccacg gttgctgggg
     7321 tcgaaccggt tgctaccact ctctagtggt ggctgggtct cagagcgtgc gcgcctccaa
     7381 gcgagaggcc tcgcggctgc gatattggat cgcacttgcc gcgcttttgc acgttcctgt
     7441 cacgtgtcta tcgagcctgg tccccctacg agacgtcacg cgccagcacc ggccgcccga
     7501 tgaagctgtc ggaatggacc tagatctcga gattgcgcac gaaggcgtgc cgccgggcgc
     7561 gcccacgacc gaagcgcgga cggaggaagc tcgcgtcgcg ccagttaaaa cagccggagc
     7621 tcatgctttg cgagggccgc gcgcattcga ggccgagccg agtcggcaac tcaacgatgc
     7681 gaactccacg cagccgaacc aatcgggtga atttcccgtt cccaatgcgc aaaccaatcc
     7741 gcggggagct gagctcacgc cttcgaacga ggggacggag cggcgcgaat cacgtccagg
     7801 gctgagcgcc gagcagctgg ggattgccgg gaccaatccg tttgtcacga ttccgagccg
     7861 tccgaccgaa acgcctgccg ggcgggctcg acgagaacag cgcgaacttt cggcacgggt
     7921 cgagcgaagc ctgaccgacg tcattagtgc ccaagatcag aagcgcggac tcgggccaga
     7981 gggagcggtg ctgcgcacgc tggaagccga agtgctcgcc agcaacacgg cgcccaattc
     8041 gagtgcggtg ttcgacgcaa tgacggacac cgccggatac ctagttggtt tgcagctcgt
     8101 ggaagcaaac tccgattccc tgttttggca ccgacttgcg gagtccactc tttctcgctt
     8161 gggcaagcaa cgtttacggg gcagccatgg caagaacgga ttgacgcttc gaattcgagt
     8221 tacatcgcgt atagctctgc ccagcggacg cgatccggga ctcgctatcg acgcctttgg
     8281 attgccgatc aaaaagggag cgggaaagcg ctcttcacgc ctttcgttgt ttgatgtgaa
     8341 accgcagctg aagagcaccg agaccacgct cccggacgga cgagtgatca gcacaccgac
     8401 gttacagctc ttctcgctcg ggcgcttgga agccgatccg gccgatattg gcgctcaacc
     8461 tcgccgcatg gtccacgcac acgtggaacg aagctggcag aattgaatcg ctcccaaact
     8521 cttggaatgg cttgacccgg agccaacgga cccctttgag gcacgataaa tcgcgtcgct
     8581 ggggacaagg cggtcctgcg aatctctgaa aacgcgcccg ggttttcccc agcatgtcgc
     8641 tcttgaacgc cggcgcgccc gggccaactg gacccgggcg cgaataccct acccaagctg
     8701 ctagccgagt ggcgcttcaa caagatgtca aacgtgacgg ggtgcgcccg ttttcgccgc
     8761 tacaaaccca acctgctccc gggaccaaat gcaggattgc gtgcacacct tgagctgctg
     8821 cagtattcac gttctcgatg gctcgcacgc ggcaatcttt gaaatgaatt tcacggaggc
     8881 tccggtcctt cgacatacct ggggcgcgag aggtacagtc agtcacacgc agatattcga
     8941 gggttttggc tggttcgggc ccgagttctg gccgcaaggg cccgttgaac gcgcccatat
     9001 ccacgctctc tggccccagg cttcagcatg ttatgaaaat catgagcatc gaatggctcg
     9061 cgctcatcgc gagcccagtc aaggtctcga ggcgggtgga tgtcacgggc acaaaaacga
     9121 atgtcacgca cacagtgaac gtcacggaag accagcagcc ctgatttgct agcttctttc
     9181 gactccgtcg gaggcgaggc tgattctgaa gtataggctg gaggtgcgag gggatttggt
     9241 gatgagagcg gcgacgatgc aggaacttct tcagcgcgag cacccgatga aaagccacga
     9301 gcaccggaat gcgaggcgag tgctgccggc aggctcgctg gaacctgttc cacacgcagc
     9361 gtcaattcct cgcaggactc taagattaac ggcaggagcg acaagaaagg gctgcccgcg
     9421 acgccttccc gaacgctact tttgtcgata ttcacgacca gcaggacccg cgtgccaatg
     9481 acttttcgca gactcttcaa ttggcgaaac agcgcagcca aatcctccga tgcccaggaa
     9541 tgccgccaac gcagcacagc aatttggccc ctgccggcgc gcgaggggga tttcctgccc
     9601 tcccgttctg acttgcaagt ttgccgttcg aggtcaggag ttcccaagcc ggtaacggct
     9661 cccgggtctt ttagcttcaa tcgtgacttt tggctgtgct cttctcccgc gccttctctt
     9721 gtaagttccc ccgtgtggtc aagttcgccc gtgctgtcgc gagtactcag gctcttcttt
     9781 cgttccacgt cgttgccctc ccgtttcgtg aagtgtcgaa atacgtacgc cttgcccagt
     9841 tctcgaatga ccttctctag aatgactttt ggtcacccga cttttggtca ccgtttcccc
     9901 cgggagttac cccagcggca ctcctcggct atacaattaa ttttgttctc gtgcaaccca
     9961 ggctcctctg agcaccagtg gattgcgccg taactccagg agtgttccgt gaatacccgc
    10021 gtcaatcgca atggcaatca tgccggcacc tccgctaacc cacgcggcgc caacctcgtg
    10081 aaaaccagga aacccaagcg tcgcggagta cctgaaccgg aaaggcgcgc cgtagcggcc
    10141 gaaatccgtg ctcttcttca agccgaagac gaaaccggtc gccgactctg gactcaaacc
    10201 tcgctcggcg aacgctgtgg tggcgtgagc caacaaatga tctacctcgc ccaagagcca
    10261 gacggtgtgg gcccgagcgt tcgagatggc ctgctcgact tcacgggcat gaccatgcac
    10321 cagctcctgg agaagcacgg gcagctcggg cgaggcggcg gcggccgcag ttaggggctc
    10381 tcgagctctc tggaccggag cccgcgctcc gccttgcggg tcttggatcg acacacggcc
    10441 gggcccggta agggtggcga cgcgttggac cccttggccc cctttcggag ggcgatggaa
    10501 gctctgtgcg acgacgggtt ccctaaagcc gcggttcaaa agatcgtggc cgagctcgcg
    10561 ttcggatctc aggttgggcg cggtggggcg gccgttcctc agcgggacgc cttggacatc
    10621 taccgaaacg cacgctcgcg tctcgagcag ggcggagatg ggaaaccgaa gagtggccgc
    10681 gccgtagctc gagcatcggg cgtacaggtg tcacgccggg aacgctgaag agggcgccga
    10741 acgcgccgta gggcgaacct ctccgatcgg gtacttgtga ccgtttgggg cgcgccctac
    10801 caaacgctca tgtcagcatc gtcaagcgtc cagcacctcg ttgaaccgtt cagtttaggc
    10861 aagtcacagc tggccaatcg cgtcgtcatg tcgccgatga cgcgctgccg cgcaatcggg
    10921 aatctgccca acgcgctcat ggcggagtac tactcccagc gagcagaagc cggattgatc
    10981 gtgacggagg gcaccagccc gtcacccaat ggcttgggct acgctcgaat cccgggttgc
    11041 ttcgacgcag cgcaggtcgc cggctggagg ctggttacgt ctgccgtaca cgacgcgggc
    11101 gggaagatct tcgtgcagct catgcacacc ggtcgtgtat cgaacgcatt gaacatgcca
    11161 ccgggcgcgc aggtggtggc gccatcagcc gtcgcgctga cgggctcgat ttggaccgat
    11221 tccgctcaac aacagcctta cgcggtgccg cgcgccatgc ttctggcaga ggttcgagag
    11281 gccatcgtgg agtacacgcg ctcggcgcgc cttgcgatcg aggcgggctt cgacggcgtc
    11341 gagcttcacg gcgccaatgg ctacttgatc gatcagttct tcaacaccgc taccaatcag
    11401 cgcaccgacg agtacggggg cagcgtagag aaccggatcc gcttcgcggt agaggttgca
    11461 cgcagcgtcg gcgaagcgat cggcttcgac cgagtgggaa tgcggctgtc cccctatgga
    11521 acgttcaatg acatgcgccc cgacccccaa atggacgaga tgtacgagcg cctagcgctc
    11581 gaactccacc gcctgggatt agtgtatctg cacgtgatca accatggcga cccgagctcg
    11641 ccggaagtcc gtcagagtgt gcgccgcgcc atccgcaaca ccttcaaggg cgcgctcatt
    11701 ttggcgggcc gatacgacgt ggcgggcgct gaacgtgagc tcgccgaagg caacgcggac
    11761 ctaatcggtt tcggcggcgc atttctctcg aacccgcgcc tcgtcagcaa gattcgcgct
    11821 ggctcaccgt tcatcccgtc taacccggcg acgtattaca cgccgggagc cgaaggatat
    11881 acggactacc ccgtttgagt tcctccgcgg gtgcgcgtga acggcagcgc aactggcgtg
    11941 gccaaaggtc acacctgatt ccaaggtggc gagcggagga agatcgtgag agcgcccgct
    12001 gagccgagct agcttcaaag agtcggctcg ggcgctctct tcaacgtctt cgagttatgc
    12061 gcgaactcct cgcaagcgtt gcgcgatttg ccggcgcgct gcccgcaagc gagaggccac
    12121 ggtatttggc gaaacctgca ttcgagtcgc aatctccagc gcggtgcagt cattcatttc
    12181 ggagagcaag aacacttccc gtttgcacgg atcgagccga gccaacgcgc ggtgcagcag
    12241 ctcgcgtcgc tcgttggaca agtagcattc gtcgggacgc ggggaatggt gctccaacag
    12301 gcccgccgcc gctccacgct ctacttcagc tagggattcc gagcgccttc gtgcggcgcg
    12361 tcgtcggtag ttgctacaaa cccgaaaggt gacagcgcgt aaccagaagt ggagaccagc
    12421 gtcgcgatcg agcctcgtga accgcgactg cagcacgatg aagacctcat gagccacgtc
    12481 ctccacgtcc gcggaagcta cgccaagccg tctcacgagg cgcatcacgc gcggcaattc
    12541 ggtgcgatac caataggcca cttgagctcg agcgagcgaa tcggcgggcg ccttgggctg
    12601 attcagcagg ttgttcgatg gtgttttgcc ggccggtccg cgtgccttcg gcgcttcgag
    12661 ggaaaggacc atttgtttcg aaaggcggcg gggtcgcggt ttagcggcag aatcttggtt
    12721 aatcacgggt ccactccgtt caacgtctgc tcgacgtgtc ggtccggagc tacggagtgg
    12781 gcggttgccc aaatgtgttg cctcgatgcg agcgctctcg ttttttttcg cgttctcgcg
    12841 gcccctcccc cgcccgcgga gcagaccaaa gaattgtcga ctctgcctgg attccgcctt
    12901 tctgcaccgc taccgcggcc tttacgctcg tgggtgtgtg cagaacggcg gtgggtcagg
    12961 gcgattgggg gcttggcaaa gctgtggctc cgacgcctca actcaccccc aatcgggcct
    13021 gggcggccag gcaatacgta agcgtagcct tcagccgttc gatcggctta acatctgggg
    13081 tcaagtctcg agccggggag ccttccgcga tgttgaatca gagcacggag agcgagctca
    13141 atagcctctt ggaggcggag cagcacaacg ccgcgattgg gctggccctg cgttgctacg
    13201 ggcccgaggt attcagctat ctcatcgtgc tgctgcgcga caagggcctg gccgaagaag
    13261 cgtattcgat gttcacggag gctctcgttc ggggcattcg aagcttcaag caacagtgcc
    13321 cattccgcgc ttgggcatac atcgtagcga agaacgaggc cctgcgcgtg atgcgccaac
    13381 gccgcagcct actcccgctc ggcagcacc
//
LOCUS       DASTKU010000312        16169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_506884, whole genome shotgun sequence.
ACCESSION   DASTKU010000312 DASTKU010000000
VERSION     DASTKU010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16169
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506884"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..450
                     /locus_tag="VFQ61_11175"
     CDS             109..450
                     /locus_tag="VFQ61_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955060.1"
                     /translation="MRCRAGTWIEGGWPADIWRAGILCCGLLGLGSSATGCARRAPGP
                     DECRAFAARVVGIRQSSDLELRSARERYLLLTRECLTTPFDREFLQCTELGRGFRACH
                     VQFEMRRRAKQ"
     gene            578..2146
                     /locus_tag="VFQ61_11180"
     CDS             578..2146
                     /locus_tag="VFQ61_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955061.1"
                     /translation="MKTAHAEETADAEGTADAEGTAELVRSGFTPRVLGGLRSTWPVA
                     LVAAIALVLLLALWSRVPTVQRLEGWFWPLGDGKLLPSLLLGVALFSFPLVIRRVADV
                     DGPKSHKRGLVLLLCLGFAYQHGLALVEHRGLDKMQERIELTGHGEFAATAARRLSVW
                     KTLVDYEHFVQAPDQRYARSKPPGQLLFYFAAAGAARQVMPLIWDPKPPQDMSLRHFR
                     LIHFATLFFPAIGLLTLIPLGYLGRLFLGERLALWPALLYLTLPGPALVQLHLDQVLY
                     PLVATTFWACCAQAARSPSGDLKPWVWCALMAWLGCFVSFSLLPMLLVGLGIVLGLAQ
                     PSAREDWLRLGRGLGVSVVAGLGLSLLAFVTIHYEPWTAYRRCMENHQEWKQWRESMR
                     WQWAKLDLIEFAYWLGVPTVALWLRAAFPSMRQGGQPARGRASVAAAANWLGGYPGLA
                     RITLLTVLLAAVLGKTEGEVARLWLFLVPTVCVGAAGALASMELGSAQARLTALSACG
                     LVWTYSLRAVQDFY"
     gene            2154..4295
                     /locus_tag="VFQ61_11185"
     CDS             2154..4295
                     /locus_tag="VFQ61_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET9955062.1"
                     /translation="MTTKLPILGCCCERSMYTARLRARLRWCVGLLSLATACSNNDAP
                     SGGTAVGCGNSAAGNSTANGGNTAKGGTAARGGSAANGGSAANSGSGNTSIAGSSTAN
                     GGAGATESEGPGEPLEPPAAWQVSDACRAQVDQLLEALSLAEKIGQMTLAERSVISAN
                     QVTQYFIGAILSEGGSYPLDGSGSAWADMTDRLHEAALQTPHAIPLIYGSDGVHGHTW
                     VPGAVIFPHNIGLGATRDRELVSDAARITARELRATGVDWTFSPVVAAPRDERWGRTY
                     EGFSEDPELAGELGAAAVMGYQGGQRLDGERNRAGTSVLATAKHFAGDGATACGSSPS
                     RLTPNALLDEGDVQLDEETFRRVAVAPYVQAIASGVGSIMVSYSSYRGVLMHRNGELL
                     TGLLKGELGFEGFVVSDYRAIEALSSSRHEQVVLAANAGIDMFMEREGWYSVITELKA
                     AVGSGEVSQSRIDDAVRRILSVKCEMGLLRPDYTGLVDRASIAEIGSEEHRAVARRAV
                     QNSLTLLENDGILPLAKSGRIHLAGSAANSVARQCGGWTMTWQGAGAEGTSVLEGFRE
                     VVQDRTEVTYSPDGTGAAGADVAIAVIGEAPYAEWFGDRVDLSLSAEDDTTLRNLSEA
                     GVPVVAVLISGRPMIITPQLPLARAWVAAWLPGSEGAGIADVLFGDVRPTAKLGHSWP
                     ARMDQIPINIGDSDYETDPPLYPLGYGLTYP"
     gene            4541..6277
                     /locus_tag="VFQ61_11190"
     CDS             4541..6277
                     /locus_tag="VFQ61_11190"
                     /inference="COORDINATES: protein motif:HMM:NF027224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HET9955063.1"
                     /translation="MSRESVHQRALGYRTLGLGSVVFCCSALIGACTPDPAKPNPQSS
                     GGAMSTGGRSSHSGGQSPGQSGASSETAGESNVGEGGAGGAGGTTPGSSGGPTGAVSS
                     TGGQGSLGGSAGRGGQDNRGGASAGGDVTEEPGGAGAGGDAGGGPSTETIVEEVEVDR
                     VWSGHPVQFPLLTRGDRQFVAYYDGNRQMTFAMRKLGEKTWQKNQLPSVTNWDSHNWI
                     RVALDSDDQIHVSGNMHNANLVYFRTRNPLDITSFSKYTSMVGTNENSMTYPEFFHNA
                     SGDLIYNYRNGGAGDGNTYFNQYSTQSQKWSRLYTLTDGEGLRNSYPVGPVLGPDGYW
                     HIVWIWRDSADAETNHDVSYARSKDLLTWESAAGAPIALPIKLSTSDVVDPVPVNGGA
                     INNNTKIGFDAENRVIISYHKFESPTGATQLYNARFEDGAWKVYQTTKRWGFRWAFQG
                     QGTLVFPIEFEGVKALRDGTLIQRYYHAYETNVEGQVGWGCFRLNPKTLVAEELIEQQ
                     LPYPRELDAATTVSSAGILASRWASDSGSSPDPSIYYMARWETLDSNRDQERPAAPEP
                     TPLKVYAFRYSK"
     gene            complement(6338..7345)
                     /locus_tag="VFQ61_11195"
     CDS             complement(6338..7345)
                     /locus_tag="VFQ61_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955064.1"
                     /translation="MLHSTLRATVSFSVLVGGMPVRHVLRIVESRCFGMLTCVGLVLA
                     LHPSRARAADSDQCIDSFEQAQELRKRGKFVAATEQLIRCSQLSCPAFIVKDCTSWFG
                     EVQAAQPSVVLSARQGAQTLNDVQVFADNVLLSERLDGRSLPIDPGQHEFRFQAAGKE
                     PVLVSVLIIEGEKHKPVIAEFPAPAPAIVESVPKPGESPAAPPDARVSSDKSSVATSS
                     DASLPERGRSVPVASYILGGAGLLAVGGGVVLRVIGANQFDDLKNSCGTTCATGDVDD
                     VKTKYTLSKVAFGVGAAALITAGALYFVNPSKSSPASSAWFIAPVIAPSGVGTVASAR
                     F"
     gene            7822..9495
                     /locus_tag="VFQ61_11200"
     CDS             7822..9495
                     /locus_tag="VFQ61_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET9955065.1"
                     /translation="MKPQAYLARSCFFALLACAHACDPDFASLTKDTGKGGTGASAGN
                     PSNGGSGGTTTEGSTQNSGGTEPRGGSSHSGGAHTAGQPGSSNSGGDGGDGGTTLPTQ
                     GGTVGTGVETGGSAGVGGDEPDPEQCSPTAPLGAGPLLRYDFDSQTGSMISDSSESEN
                     HGTLNGGRWVSAARHGGGIEFYAEDQYIALPERLIDGLDRLTVSAWLNLAANPAGSTL
                     FDFGADSANHVFLKTNTGTGISFGVVLKGQEQRAFSARALPLGVWQHVAAVIQRGRVI
                     LYINGLEQARADVTFSASSLSGQLKNWVGRSQDGSGNLSGALDDLRVYGRALERGEIQ
                     DLATPDYRYFPFDEGCGTEIHDISPGALKGELPKGGTWTTGRVGGALELDGKQQYVML
                     PAGIVESCDELTLAFWAQRTRNTGVSEHILDFGQNQRTFLYVTPQARGAGLARFAIKL
                     NGDEDDITAEQALNGNGPLSVGLWQHVAVTLRAGVGTLYVDGTQYDSRPIALKASALG
                     KTMINTIGRSQYFNVSLGPDSFYAGRIDELRIACRAFSAEEVGLISMLR"
     gene            9714..11036
                     /locus_tag="VFQ61_11205"
     CDS             9714..11036
                     /locus_tag="VFQ61_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET9955066.1"
                     /translation="MSWWLGLSLVAGGSFVISACGDDDDTPAPCSGSSCASGGKTGSG
                     GARSGGSSSVSGGETQGTSGGATAKGGTGNTTSATGGSTSGGSTSGGNGDGGTTAQPE
                     GGTQANGGTQANGGTTQGTSPEGGMAGGGAGGDAGGDAGAGQGGATGQGGTTSVVGGG
                     GAGGDTDPPVLAFNVTQGPNQLNIAPGGVNFATLDVQNQSEIPIQVTVAATSPNGAEA
                     TPLMQVLPLAVDQKSTATLRLNGTNVTTGTGALSVTVSASGIDSISRTFGLTFSADMA
                     QNLFAATSPWPKAIASSYQWPDDPTKQLPPKNAFDGQAGTFWVSKGRNAGEGPKPDAP
                     EWLGVDFGAAVTVGKVVMTPRAQRGPTAYTVEVSDDQMAWDKVAEEPTAADVPVTTTL
                     ASPVTKRYLRLLITGSRDPGNSTDCMPPCDPRNVQIISLEVFPPAASM"
     gene            11134..12900
                     /locus_tag="VFQ61_11210"
     CDS             11134..12900
                     /locus_tag="VFQ61_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9955067.1"
                     /translation="MTAGLTNQGLRSEVSLDSPNTIGKYRLVAELGRGGAACVYLAVA
                     RGQSGINKLVVLKRLLPQLAEDPIAVSSFLDEARLAAQLNHPNVVQTYEVGSEGEHYF
                     LVMEYLEGQSLSRILRAVGYTFPQAIHLRILVDVLEGLHYAHELASYEGAPLRLVHRD
                     VSPQNVLVTYDGQVKVLDFGIAKATISSTHTATGMMKGKVPYMAPEQMNGGDLDRRAD
                     IFSVGSMLWGAVAGQKLWKGLTDVQIVSRVLSGQIPSPREVNPNCPAELARITQRALA
                     LNPDDRYSTAHEFQQDLEQYLERHASVRRRDVGLFVSQTFTEPRAELKSLVERELSMV
                     SSEQEIAEQEVASSLRRGFSVSRDNPATIEAFRAMATQPVPLPEPQPSRPRAKRRWWA
                     AATLALLGFAGWWHLRSEPPAAPTAARSLQVQPKALVVDPTPITQGAVVATSASSEPG
                     PDAAPIARAKIRVSVSPQQAQLTLDGTRVTAEDLLELPIDGREHVLMASAPGYETMTR
                     NFEVTSALETIEVALNPARRSAASTPVQNKRPAVSAASVPASDADDPIDMRVAEPAAR
                     KDCDNPFFVDARGIKRVRPECR"
     gene            complement(13103..15682)
                     /locus_tag="VFQ61_11215"
     CDS             complement(13103..15682)
                     /locus_tag="VFQ61_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET9955068.1"
                     /translation="MAPPGDTVTLSLDRFAAEARGVIVAAQALADERRHLQVEPLHVF
                     ASALARNPSVAEVLRSSGADPAEFSALMERALGGLPRSNEPAFLSPATLELLRRTERE
                     AEREKREQILVQDVLNALSQELRGALGEILSAFRIGPGALRPHMSALRSRPRDEPAAA
                     EPLAPTEYLRRMPAQDSNPGSVAVVGRAVELRRLLTVLVRSNKSHPLLVGEPGVGKRA
                     LVRLLAQRIAQGAVPDVLTRAGVFELELAAIAAGARLRGEVEDRMRKLLNDLRSRREP
                     SVWFVHAIDQLFGGTGPLAGLPDLLRPVLERGELRLISTTTPDGLRKLHEKDPHFARL
                     LTELNVDEPSPDQALEMVRAAAALLEEHHRVAIAEDAIASSVELSRRYLQERHLPDSA
                     IDLLDESAAQRRLTIDSLPEAADFELRRFESLEAQLALDGDPREAERHPVRLRLQAEH
                     RQLSPKVAETRKAAEARRQSRRKLLEVQADVAQQRAAQSELSPILQAKLAEAEREAEA
                     VGAATKPPLVNAEDVAATLAAWTQIPVQRMMEGEADRLLRMDERLGERVIGQDHAVSS
                     VARAVRRGRVGLRDPRRPIGSFLFLGPSGVGKTELAKALANLLFDDERALTRLDMSEF
                     MERHMAQRLIGAPPGYADSEQGGFLTEAVRRRPYSVLLFDEVEKAHQDVFNLLLQLLD
                     EGRLTDGRGRTADFSNTVVILTSNIGSDRVLATEPALFDSEPGREALRDVLLDKLREF
                     FRPELLNRLEEVLVFRPLSRAHLQKIVDLELKNVSKLLDTRSITLQATDAAKARLVEL
                     GYEPALGARPLRRTILHHVQDPLAEALLAGKFLPNSQVTLDVAGDELVLRPQTDSPAQ
                     PSS"
     gene            15798..>16169
                     /locus_tag="VFQ61_11220"
     CDS             15798..>16169
                     /locus_tag="VFQ61_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET9955069.1"
                     /translation="MPSFDEFPPWFWYGVLVPFGLLWGSFLNVVIYRLPRDMSVVRPG
                     SRCPACGTPIRPWNNVPVLSYLFLRGRASCCQAPISPRYPLVELSGGLLAWAVLRCIV
                     FELPGGAGLGQALGLFGLYLGL"
BASE COUNT         2900 a         4804 c         5467 g         2998 t
ORIGIN      
        1 gctgctcacg ttgactcgca aactcgacga ccgcctcgac cgccggggca ttcagtgagc
       61 ccccgccagc ggtgccgcgc gcgccgccga gcttactacg ttcgaggttt gcggtgccgc
      121 gcgggaacgt ggatcgaagg cggctggccc gctgatattt ggcgcgcggg catcctgtgt
      181 tgcggattgt tggggctcgg ctcgagcgcg accggctgcg cgcgccgcgc gcctggcccc
      241 gacgaatgcc gcgccttcgc ggcgcgtgtc gtggggattc ggcagagttc ggatctggag
      301 ctccgctccg cgcgagaacg ctacctgttg ctcactcgcg agtgcttgac cacgcccttc
      361 gatcgagaat ttctgcaatg tacggagctc gggcggggct tccgtgcatg ccacgtgcag
      421 ttcgagatgc gtcgccgcgc gaaacaatag ttcaatcgaa acccgagcgg ccgtagcggg
      481 aacgccgctt gacgccgcag agggggcgag cgatcaggcg ccgtggaaac tcgcaatctg
      541 cgcacttcag ggtggagccc gtgaaccgcg gagcacgatg aaaaccgcgc atgctgaaga
      601 aacagcggat gccgaaggaa cagcggatgc cgaaggaaca gcggagctag ttcggagcgg
      661 cttcacgccc cgcgtgctcg gcggtttacg cagtacttgg cccgtcgctc tggtggcagc
      721 gattgccctc gtgttgctgt tggctctgtg gtcgagggtg ccgaccgtgc agcgcttgga
      781 aggctggttc tggccactcg gtgacggcaa actgctgccg agcctgctcc tcggcgttgc
      841 actcttcagc ttcccattgg tgattcgacg agtcgccgac gtggatggtc cgaagagcca
      901 caagcgtggc ctcgtcttgc tgctgtgctt gggcttcgcg taccagcacg ggctcgcgct
      961 agtcgagcac cgcgggctcg acaagatgca ggagcgcatc gagctgaccg ggcacggtga
     1021 atttgcggcc accgccgcgc gacgcctgag cgtttggaag acgctggtcg attacgaaca
     1081 cttcgtgcaa gcgcccgatc aaagatatgc gcgatcgaag ccgccggggc agctgctctt
     1141 ttacttcgcg gcggcggggg cggctcgcca ggtaatgcca ctcatctggg atcccaagcc
     1201 gccccaagac atgagtctac gtcacttcag gctcattcac ttcgcaactc tgttttttcc
     1261 cgccatcggg ctgctcacgc tcattccgtt ggggtacttg gggcgactat tcctcgggga
     1321 gcgtctcgcg ctctggccgg cgttgctcta cctcacgctg cccgggcccg cgctcgttca
     1381 gctgcacctt gaccaagtgc tttatccgct cgtggcgacc acgttttggg cttgctgcgc
     1441 gcaggcagcg cgctcgccca gcggtgactt gaagccgtgg gtgtggtgcg cgctgatggc
     1501 ttggctcgga tgcttcgtgt ccttctcgtt gttacccatg ctcttggtag gcttgggaat
     1561 cgtgttggga ctcgcgcagc ctagcgcccg tgaagactgg ttacgtctag ggcgcggctt
     1621 gggcgtctct gtcgtcgcgg ggcttggact cagcctccta gcgttcgtca cgatccacta
     1681 cgaaccttgg actgcgtacc gccggtgcat ggagaaccac caagaatgga agcaatggcg
     1741 ggagagtatg cgctggcagt gggccaagct cgatctgatc gagttcgctt attggctcgg
     1801 ggttccgacc gtggcgttgt ggcttcgcgc ggcgttccca tcgatgcggc aaggaggaca
     1861 gcccgctcgg ggtcgtgcgt ctgtggcagc ggcggcgaac tggctcggcg gctacccggg
     1921 cctggcccgc atcacattgc tcacggtttt gctcgccgcg gtgctcggca agaccgaggg
     1981 cgaggtcgcg cgtctgtggc tgtttttggt cccgaccgtg tgtgtcggcg cggccggagc
     2041 gctggcgagc atggagctcg gcagcgcgca agcgcgcctg acggcgctca gtgcctgtgg
     2101 cctggtgtgg acctactcac ttcgagccgt gcaagatttc tactgagagc gccatgacaa
     2161 ccaaacttcc catcctcgga tgctgctgtg agcgcagtat gtacacggct cgtttgcgag
     2221 cacgtctgcg ttggtgcgtg gggctgctga gtctggccac ggcttgttcg aacaacgacg
     2281 ctcccagcgg tggcacggcc gttggttgcg gcaactcagc ggctggaaac agcacggcca
     2341 acgggggtaa cacggccaag ggtggcactg cggctcgggg cggcagtgct gctaacggcg
     2401 gcagtgctgc taacagcggc agtggcaaca ctagcatcgc cggcagtagc accgcgaacg
     2461 gcggcgcggg ggctacagag tcggaggggc caggcgaacc gctcgaaccg cccgcggctt
     2521 ggcaggtttc ggacgcgtgc cgagcgcagg tcgatcagct gttggaggcg ctcagcttgg
     2581 cggaaaagat cgggcagatg acgctcgctg agcgcagcgt catctctgcg aatcaggtga
     2641 ctcaatactt cattggagca atcctcagtg aagggggctc gtatccgctg gatggctctg
     2701 gcagcgcgtg ggcagacatg accgaccgct tgcacgaggc ggcgcttcag acaccacacg
     2761 ccattcctct catctacggc tcggacggtg tgcatggaca cacgtgggtg ccaggagcgg
     2821 tgatctttcc gcacaatatc ggcttgggcg ctacccgcga tcgcgagttg gtgagcgatg
     2881 ctgcccgcat caccgcacgt gagttacgag ccaccggcgt ggattggacc ttctctcccg
     2941 tcgtggccgc gccgcgagac gagcgctggg gccgcaccta tgaaggcttc tccgaggatc
     3001 cggaactagc cggagagctc ggcgcggcgg cggtcatggg ataccaagga ggccaacgcc
     3061 tggacggtga acgtaaccgc gccggcacct cggtgctggc gacggccaag cacttcgcgg
     3121 gtgacggcgc gaccgcctgt ggtagctcgc cgagtcggct gactccgaac gcgctgctcg
     3181 atgagggcga cgtgcagctg gacgaggaga cctttcgtcg cgtcgccgtg gccccctatg
     3241 tgcaagccat cgccagtggc gtcggttcaa tcatggtgag ctactcgagt tatcgcggtg
     3301 tgctcatgca tcgaaacggc gagctcctga ccggcctgct caagggcgag ctcgggttcg
     3361 agggcttcgt ggtctcagat taccgagcca tcgaggcgct atcttcgagt cgccacgagc
     3421 aagtggtgct ggcggcgaat gccggtatcg acatgttcat ggagcgtgag ggttggtaca
     3481 gcgtgattac ggagctgaaa gccgccgtcg gaagcggtga ggtttcgcag agccggatcg
     3541 acgacgccgt gcgacgcatc ctgagcgtga agtgtgaaat gggcttactg cgcccggact
     3601 acaccggctt ggtcgatcgc gcatcgatcg ccgaaattgg ttcggaggaa caccgcgccg
     3661 tcgctcggcg agccgtgcaa aactcgctca cgttgctcga gaacgacggc atcttgccgc
     3721 tcgccaagag tggacgcatt cacctcgccg gctcggccgc aaacagcgtc gcgcgacagt
     3781 gcggcggctg gaccatgacg tggcagggcg ccggtgccga aggtacctcc gtcctagaag
     3841 gctttcggga ggtggtccag gaccgcaccg aggtaactta ttcgccggat gggaccgggg
     3901 ccgcgggtgc cgacgtcgcg atcgccgtga ttggagaggc gccctatgcc gagtggttcg
     3961 gtgatcgcgt ggacctgagc ctatcggccg aggacgacac gaccctacgt aacctgagcg
     4021 aagcaggagt tcccgtggtg gccgtgttga tttcgggccg tccgatgatc atcactcctc
     4081 aattgccgct cgcccgagct tgggtggccg cctggttgcc gggaagcgag ggggctggga
     4141 tcgctgacgt gctgttcggg gatgtccggc ctactgcaaa gctgggccac tcttggccgg
     4201 cccgcatgga ccagatcccg atcaatatcg gggattcgga ttacgaaacg gatcctccgt
     4261 tgtacccact cggctacggc ctcacctacc cgtgaggcgg atgctgaacc agcaatccaa
     4321 gttggattga gcttcagtgg ggtcggcatc agtcccaaat catgccgaca tatgctccgc
     4381 cttgaggttg ataactcgag gttgatacgg agccatgcgt cttgctacgc tgggcctcat
     4441 gaccacgagg ggcgtcaccc gcagggctcg ttggctgatg catgactcgc agaacgattg
     4501 ttcgtcggcg ccaaagccag gtcgggtgag ccgaggtcgg gtgagccgcg aatccgttca
     4561 ccaacgagcg ctgggctatc gaacgctcgg gctgggctcc gtggtcttct gctgttcggc
     4621 gctcatcggc gcgtgcaccc ctgatccagc caagcccaac ccgcaaagtt ctggcggtgc
     4681 catgagcacc ggtggaagat cgagccactc gggtggccaa tcgccggggc agagcggggc
     4741 tagttccgag acggctggcg agagtaatgt tggcgaagga ggcgcgggcg gcgcaggcgg
     4801 tacgactcct ggctcgagtg gcggcccaac cggtgccgtg tcctcgaccg gagggcaggg
     4861 cagcctaggc ggcagcgccg gtcgcggtgg acaggataac cgtggcggcg cttcggcagg
     4921 gggcgatgtc acggaagaac cgggcggcgc gggagcgggt ggagatgccg gtggcggtcc
     4981 ctccactgaa actatcgtgg aagaggtcga ggtcgaccgc gtgtggtccg ggcatcccgt
     5041 gcagtttccg ctgctgacgc gcggtgatcg tcaattcgtc gcctactacg atggcaaccg
     5101 gcaaatgacc ttcgccatgc gtaagcttgg cgaaaagact tggcaaaaga accaactgcc
     5161 gtccgtgacc aattgggata gtcacaattg gattcgagtc gcgctcgact cggatgatca
     5221 gattcacgtg tccgggaaca tgcacaacgc aaacctggtg tacttccgga cgcgcaatcc
     5281 tctggatatc acctcgttca gcaaatacac cagcatggtg ggtacgaacg agaacagtat
     5341 gacgtacccg gagttcttcc acaatgcgag tggggacctg atttacaatt accgcaatgg
     5401 cggggcgggt gacggtaata cctatttcaa tcagtactcg actcagtccc aaaagtggtc
     5461 gcgcctgtat accctcacgg acggcgaagg gctgcgaaac tcgtatccag tgggccccgt
     5521 gttggggccg gacggttatt ggcacatcgt ttggatttgg cgagatagcg ccgacgccga
     5581 gaccaatcat gatgtctctt acgcgcgcag caaagatctg ctgacttggg agtccgccgc
     5641 cggtgcaccg atcgcgctgc cgatcaaact gagtacgtcg gatgtcgtgg atccggttcc
     5701 ggtgaacggc ggtgcgatca acaataatac gaagattggg ttcgacgccg agaaccgcgt
     5761 gatcatcagc tatcacaaat tcgagagccc cactggcgcg actcagctct acaatgcgcg
     5821 cttcgaggat ggcgcctgga aggtgtatca gacgaccaaa cgttggggct tccgttgggc
     5881 gttccaggga cagggaacct tggtgttccc gatcgagttc gaaggcgtca aggcgttgcg
     5941 ggatggcacg ctgattcagc gctattatca cgcttacgaa accaacgtcg aaggccaggt
     6001 ggggtgggga tgtttccgac tcaacccgaa gacgctcgtg gcggaggagc tgatcgaaca
     6061 gcagctcccg tatccccgag aattggatgc cgccacgacc gtgagctcgg ccggcattct
     6121 ggcttcacgc tgggcgtcgg attctgggtc tagcccggac ccgagcattt actacatggc
     6181 gcgctgggag acgctcgatt cgaatcgcga tcaggagcga cccgcggcgc cggagccaac
     6241 tcctttgaag gtttatgcct tcaggtattc caagtgaccc ttggagttcg gccccgaggc
     6301 gtgacggctc acgccgcata gtgcgggccg actcccacta gaaccgagcg ctcgcgacgg
     6361 tgccgacgcc cgagggggca atgacgggtg cgatgaacca ggcgctgcta gcaggcgatg
     6421 atttcgaggg gttcacgaaa tagagcgccc ccgccgtaat caacgccgcg gcgccaaccc
     6481 cgaaagcaac cttggacaag gtgtacttcg tttttacgtc atcgacgtcc cccgtggcac
     6541 acgtggttcc gcaagaattc ttcaaatcat cgaattggtt ggcgccgatc actcgcaaca
     6601 ctacgccacc cccgaccgcc aacaggcctg caccacccaa tatataactc gccacgggaa
     6661 cgctccgccc tcgctccggg agacttgcat ccgaagatgt agcaacagat gacttgtcgg
     6721 atgaaacccg ggcatcggga ggagcagctg ggctttcgcc tggcttggga accgattcca
     6781 caatggccgg agccggcgcg gggaactcgg caattaccgg cttgtgcttc tcgccttcga
     6841 tgatcaggac cgaaactagc accggctcct tcccggccgc ctgaaaccgg aactcgtgtt
     6901 gacctggatc gatgggcaac gagcgcccat ccaaacgctc actgagcaac acattgtccg
     6961 cgaacacctg cacgtcattc agggtttgcg ccccctgtcg cgcactcaac acgaccgacg
     7021 gctgggctgc ctgcacctca ccgaaccagc tcgtgcaatc cttcacgatg aaggccggac
     7081 agctgagctg ggaacaacga atcagctgct cagtagctgc gacgaatttg ccgcgtttgc
     7141 gcagttcttg cgcttgttcg aagctatcga tgcattgatc cgagtcggcg gcccgggcgc
     7201 gcgacggatg caaagccaac accaggccca cacaggtcag cattccaaag cagcggctct
     7261 ctacaatgcg caagacgtga cgcacgggca tgcctccaac caaaaccgag aatgaaactg
     7321 tcgcccgcag ggtactgtgc agcatcgaac ttgtcactca cgggaggtct tcgcgaaccg
     7381 taattcgcac ttggtaccta tgactttcga gattaaccta ggcgcactgt gaatgggcgt
     7441 tctgctccga tgggctgcgg cggcgcgcac gcgccaaaga aagcgcgggc ttgcgagctg
     7501 gacagcagct caggtctccc gggccgctgg gtagagtcta caatctgctc gcttcgggcc
     7561 gcggtgcggc ggtcccctcg cgttgataac ctgatttctt acggttcgat actgacctga
     7621 tgatggccag aaactaggca accccgccgc aagccaagga caatgagcag ctcgtcttgc
     7681 tggttactca cctcgacgcc gctttggaac tcgaaactcg acgacacgca gcattcaacc
     7741 ctacttcgaa tccgatccag catcgaatcc aatccaactt ggagtgctgg gctcaatgac
     7801 tgaatgggct cgagaaaacc cgtgaaaccc caggcttact tagcgcgctc ttgttttttt
     7861 gctttgctgg cgtgtgcgca tgcctgcgat ccggacttcg cttctttgac gaaggatact
     7921 ggtaaaggcg gcacgggggc atcggccggc aacccatcca acggcggctc gggcggcacg
     7981 acaaccgagg gttcaactca gaattccggg ggaaccgagc cacgcggtgg aagcagccat
     8041 tcgggcggag cacacactgc cggccagccg gggtcctcga actctggggg agacgggggc
     8101 gacggcggga ccacgctgcc aacccagggt gggaccgtgg gaacgggcgt cgaaacgggt
     8161 ggcagcgcgg gcgtgggagg cgatgagccg gatccggagc agtgctcgcc gacggcgccg
     8221 ctcggtgcgg gaccgctgct tcgctatgat ttcgattcgc aaaccggaag catgatttca
     8281 gactcttccg agtcggagaa tcacggcaca ctcaacggcg gtcgttgggt atcggctgct
     8341 cgtcacggag gcggcatcga gttctacgcc gaggatcaat acattgcctt acccgaacgt
     8401 ctgatcgatg gactcgatcg cctgaccgtg agcgcgtggc tgaacttggc tgcaaatccg
     8461 gcgggctcga cgctgttcga cttcggggca gactccgcga atcacgtgtt tttgaagacc
     8521 aacacgggaa cgggcattag cttcggggtc gtgctcaaag gccaggaaca gcgtgcattt
     8581 tcggcgcggg cgctacccct aggggtctgg cagcacgtcg cggccgtgat ccagcgcggc
     8641 cgcgtgattt tgtatatcaa tgggctcgag caggctcgag cggatgtgac gttttcggcc
     8701 tcgtccctgt cagggcagtt gaagaattgg gtggggcgca gccaggacgg cagtgggaac
     8761 ttgagtgggg ctctggatga tctccgcgtt tatgggcgtg cgctcgagag gggcgagata
     8821 caagacctag cgacgcccga ttacaggtac ttcccgttcg acgagggctg cggcactgaa
     8881 atccacgata tttcccctgg cgcgctgaaa ggcgagttgc caaaaggcgg aacatggacc
     8941 acggggcgcg tgggcggcgc gctcgaactc gacggcaaac aacaatacgt aatgttgccg
     9001 gcaggtatcg tggagagttg tgacgagctc acactcgcct tctgggctca acgtactcgc
     9061 aacaccggtg tttccgagca catcttggat ttcggccaga atcagcgcac attcctgtac
     9121 gtcacgccgc aggcgcgagg ggctggtctc gcgcgtttcg ccatcaaact gaatggcgac
     9181 gaggacgata tcacggcgga acaagctctg aacgggaacg ggccgctctc cgtaggcctg
     9241 tggcaacacg tggcggtgac gttgcgagcc ggcgtcggaa ccctgtacgt tgatggaact
     9301 cagtacgatt ctagaccgat cgccctcaaa gcctctgcct tgggcaagac catgatcaat
     9361 accattggtc ggtcacagta cttcaatgtg tcgctcggcc ccgattcctt ctatgccggg
     9421 cggattgacg agctgagaat cgcgtgccgc gctttctcgg cggaagaggt gggactgatt
     9481 tccatgcttc gctgatgctt tcaggcttgg ctgttcgcag tgacaccctg agggtacgaa
     9541 tcctaccttc atgagtgata gttgcacttc atggatgaca ttggtgcgtg cattctggca
     9601 tgacgtgtcg aggtggtagg aatgcgcggg ggtgagcccc gccgtccgcg cctcattggc
     9661 tgacttcatc aaactcgaac caagatgcac gaggtagcgt gatgcttcgg aacatgtcgt
     9721 ggtggctggg gctttcgttg gtggccggcg gcagcttcgt aatttcggcg tgcggtgatg
     9781 acgacgatac gccagcgccg tgctcgggca gctcttgtgc gagcggcgga aagacgggca
     9841 gcggtggggc gcgcagtggc ggtagctcat ccgtgtcggg cggagagacc cagggcacga
     9901 gcggcggcgc caccgccaaa ggcggaaccg gtaacacgac gagtgctact ggtggcagca
     9961 cctcgggcgg cagtacgtcg ggtggcaacg gagacggcgg aaccaccgca caacctgaag
    10021 gcggaacgca agccaacggt ggaacccaag cgaacggcgg caccacgcaa ggcacctctc
    10081 ccgaaggcgg tatggccggc ggcggagcgg ggggcgatgc gggtggagac gcaggcgccg
    10141 ggcagggcgg tgcaaccgga caaggcggaa ccacgtcagt cgttggtggt ggaggagccg
    10201 gcggtgatac cgatcctccc gtccttgcat tcaacgtcac gcaaggcccg aaccaattga
    10261 acatcgcgcc cgggggagtg aacttcgcga ccctggacgt gcagaatcag tccgaaattc
    10321 cgatccaagt gaccgtggcc gccacgagcc cgaatggggc cgaggccacg ccactcatgc
    10381 aggtgctgcc gttggcggtg gatcagaaat ccacggcgac tctgcgcctc aacggcacca
    10441 acgtcacgac cggtacggga gcgctctcgg tcaccgtctc ggcgtcaggc atcgattcca
    10501 tttcgcgcac cttcggcctg actttctcgg cggacatggc gcaaaatctt tttgcggcca
    10561 cctcgccgtg gccgaaggcc atcgcgagct cgtatcagtg gcctgatgat cccacgaagc
    10621 agctgccgcc caagaacgcc tttgatgggc aggcgggcac gttctgggtc tcaaaaggaa
    10681 gaaacgcggg tgagggcccc aagccagacg ccccagagtg gttgggcgta gattttgggg
    10741 ccgccgtgac cgtcggcaaa gtcgtgatga cgccccgagc gcagcgcggg ccaaccgcct
    10801 acacggtcga ggtctcggat gaccagatgg cttgggacaa ggttgcagag gaacccaccg
    10861 cggcggacgt tccggtcacc accacgctgg cgagcccagt cacgaagcgc tatctgcgtt
    10921 tactgatcac gggcagccga gatccgggca actcgacgga ctgcatgccc ccctgcgatc
    10981 cacgcaatgt tcaaatcatt tcgctagagg tcttcccccc ggcggcgagc atgtaacgcg
    11041 cccgctggga tgctgctttg ccttgcgagt ccccgtagca ttggggctcg caaggcagca
    11101 tcatagcccc cgacaccgag cccccagatg cccatgactg ctggattaac caaccaaggt
    11161 ctacgtagtg aggtgagcct cgactcgccc aatacgattg ggaagtatcg gctggtggct
    11221 gaactcgggc gcgggggcgc ggcgtgtgta tacctggccg tggcgcgcgg gcagagcggc
    11281 atcaataagt tggtggtgct caagcggctg ctcccgcagc ttgccgaaga cccgatcgcg
    11341 gtgagcagct tcttggatga ggctcgcctc gcggcgcagc tcaaccaccc gaacgtggtg
    11401 cagacctacg aggtgggctc ggaaggtgag cactacttct tggtgatgga gtacctggag
    11461 gggcaatcgc tgtcgcgcat cctgcgcgcg gtcggctaca ccttcccgca agccattcat
    11521 ttacgcatcc tcgtggatgt gctggagggg ttgcattacg cgcacgagct cgcgtcgtac
    11581 gaaggcgcgc ccttgcggct cgtgcatcgt gacgtgtctc cgcaaaatgt gctcgtgacc
    11641 tacgacggtc aggtcaaggt gctcgacttc ggcatcgcca aggcgaccat ctcttcgacg
    11701 cacaccgcga ccggcatgat gaagggcaag gtgccgtaca tggcgcccga gcaaatgaac
    11761 ggcggcgatc tcgatcggcg cgcggacatc ttctcggtgg gctcgatgtt gtggggcgcc
    11821 gtcgccggtc agaagctctg gaaaggcctg actgacgtgc agatcgtgtc gcgcgtgttg
    11881 agcgggcaaa tcccgtcacc ccgcgaagtg aacccgaact gccccgctga gctggcgcgc
    11941 atcacgcagc gcgcgttggc gttgaacccc gacgaccgtt actccaccgc gcacgagttc
    12001 caacaggacc tcgagcagta cttggagcgg cacgcctcgg ttcgacggcg agacgtgggc
    12061 ctgttcgtgt cccagacgtt caccgagccg cgcgccgagt tgaagagcct ggtcgagcgc
    12121 gagctctcga tggtctcttc ggagcaagag atcgccgaac aagaagtggc ctcgtctctt
    12181 cggcgtggtt tcagcgtgag ccgcgataac ccagccacga tcgaggcgtt ccgtgcgatg
    12241 gcgacgcaac cggtgccgtt gccggaaccg cagccttctc gtccccgcgc gaagcggcgc
    12301 tggtgggcgg cggccacgct ggcactcttg gggtttgcgg gttggtggca tttgcgttcg
    12361 gagccgcccg cggccccaac ggctgcacgt tcattgcagg ttcagcccaa ggcgctggtc
    12421 gtcgatccga cgccaatcac gcaaggcgcg gtggtagcga cgtccgcgag ctcggagccg
    12481 ggccccgacg ccgcgccgat agcgcgcgcg aagattcgtg tgtcagtgag ccctcaacaa
    12541 gcgcaactga ctctcgatgg cacgcgcgtg acggctgaag atttgctcga actcccgatc
    12601 gacggccgcg agcacgtgct gatggcgagc gcgccgggat acgagacgat gacgcgtaac
    12661 ttcgaggtca cctcggcgct agagaccatc gaagtcgcat tgaatcccgc gcgacgttcg
    12721 gcggcgtcta ctcccgtgca gaacaagcgc cccgccgttt ccgctgcgtc tgtgccggca
    12781 agcgacgcgg atgatccgat cgacatgcgg gtagcggagc cggctgcacg caaggattgc
    12841 gataatccct ttttcgtgga tgcgcggggc atcaagcgcg tgcggcccga gtgccgttga
    12901 agaccgcagg aaagagtcga agcctcgctg cggcgtgacc tctagtgcgg ttgcggtggg
    12961 ggccggcgcg gtgaaggggg attagaggga agagtgactg cgctggcgag tggagtctgt
    13021 cggtcagcgc gcacgttatc tcgcggccaa cgcagaatgc ggcgacgcac gtgccctaac
    13081 cgacttgctg tgtcgaccca gattacgagc tgggttgtgc gggggagtcg gtttgagggc
    13141 gtagtacgag ttcgtcgccc gcgacgtcga gggtgacctg cgaattgggc agaaatttgc
    13201 cggcgagcag cgcttccgcg agcgggtctt ggacgtggtg aagaatggtg cggcgtaagg
    13261 ggcgtgcccc gagcgcgggc tcgtagccga gctcgaccaa gcgcgctttg gcggcgtcgg
    13321 tggcttgcaa ggtgatcgag cgggtgtcga gcagcttgct gacgttcttg agctcgagat
    13381 cgacgatctt ctgcaggtgc gcgcgactca aagggcggaa gacgagcact tcttcgaggc
    13441 ggttcaggag ctcggggcga aagaactctc gcagtttgtc gagcagtaca tcgcgcaggg
    13501 cttcccgacc gggttcgctg tcgaacaagg cgggctcggt ggcgagcacg cgatcggagc
    13561 cgatgttgct ggtgaggatg acgacggtgt tcgaaaagtc agcggtgcgg ccgcggccgt
    13621 cggtcaggcg cccttcgtcg agtagctgca agagtaggtt gaaaacgtct tgatgcgcct
    13681 tctcgacttc gtcgaagagc aatacggagt aggggcgtcg gcgcacggct tcggtgagga
    13741 agcccccttg ttcgctgtcg gcgtagcctg ggggagcgcc gatcaagcgc tgggccatgt
    13801 ggcgctccat gaactcgctc atgtccaggc gggtgagggc gcgctcgtcg tcgaatagta
    13861 ggttcgcgag cgccttggcg agctcggttt tgccgacgcc gctcggcccg aggaataaga
    13921 acgagccgat cgggcggcgc ggatcacgta ggcccacgcg gccgcgacgg accgcgcgcg
    13981 cgactgagga gacggcgtgg tcttgcccga tcacgcgctc accgagtcgt tcgtccatgc
    14041 gcagcaggcg atcggcttcg ccttccatca tgcgctgcac gggaatctgg gtccatgcgg
    14101 cgagggtcgc ggcgacgtcc tcggcgttga ccaggggagg tttggtggcg gcgcccacgg
    14161 cctccgcttc gcgctcggct tcggctaatt tcgcctgcaa gatagggctg agttcgcttt
    14221 gagcggcgcg ctgctgggcg acgtcggctt gtacttccaa gagcttacgc cggctctggc
    14281 gccgcgcttc cgcagctttg cgcgtctcgg cgaccttggg gctcagctgg cgatgctcgg
    14341 cttgcaaccg taaccgcacg gggtgacgct cggcctctcg aggatcccca tccaaggcaa
    14401 gctgggcttc caaggattcg aagcggcgga gctcgaagtc ggcggcttcg ggtaggctgt
    14461 cgatggtcag acgacgttgg gcggcgctct cgtccagcag atcgatcgcg gagtcgggta
    14521 gatgccgctc ctgcaggtaa cggcgggaga gctcgacgct gctggcgatg gcgtcttcgg
    14581 cgatggccac gcggtggtgc tcttcgagca gggccgcggc cgcgcgcacc atctcgaggg
    14641 cttggtcagg gctgggttcg tcgacgttca gttcggtcag gagccgggcg aagtgcgggt
    14701 ccttctcgtg gagcttgcgc aggccgtccg gggtggtggt ggagattaag cgcaactcac
    14761 cgcgttcgag cacaggacgc agtagatcgg gcaggccagc gaggggaccc gtgccgccga
    14821 acagttgatc gatggcgtgg acgaaccata cgctgggttc acggcgtgag cggaggtcat
    14881 tcagcagctt gcgcatgcgg tcttcgactt ctccgcgcag gcgcgctccg gcggcgatgg
    14941 ccgcgagctc gagctcgaag acacccgcgc gggtgagcac gtctggcacc gcgccttggg
    15001 cgatgcgctg ggcgagcagg cgtacgagcg cgcgcttgcc gaccccgggt tctccgacga
    15061 gcagcgggtg ggatttgttg ctgcgcacga ggacggtgag caagcggcgc aactcgacgg
    15121 cgcggcctac taccgcgacg ctccccggat tggagtcctg ggcaggcatg cgtcgtagat
    15181 attccgtggg cgcgagcggc tcggctgcgg ccggttcgtc acggggccgg ctgcgcaagg
    15241 cagacatgtg cgggcgcaga gcaccagggc cgatgcggaa ggcactcaag atctcaccga
    15301 gcgcaccgcg gagctcttgc gacagggcgt tcaaaacgtc ttgcaccagg atctgctcgc
    15361 gtttctcgcg ctcggcttcg cgctcggtgc ggcgtagcag ctccagagtg gccggggata
    15421 gaaacgcggg ctcgttggaa cgcggcaggc cgccgagcgc ccgctccatc aaggcgctga
    15481 attcggccgg atccgcgccg ctactgcgca atacttcggc tacagaggga ttgcgcgcaa
    15541 gcgcgctcgc gaacacatgc aaaggctcga cctggaggtg gcggcgttcg tctgccaatg
    15601 cctgtgcggc cacgatcacg ccgcgcgcct cggcagcaaa acggtccaac gagagggtga
    15661 cggtgtcgcc agggggggcc atgagcgagc aaggctagag caacgttcgc cctgagacca
    15721 gtatggcgac gagcaaggcc gtacggctga gttgcacggc tgagttgcac ggctgagttg
    15781 tgccatgatg ggggcacgtg ccgagcttcg acgagtttcc cccttggttc tggtatggcg
    15841 tgctggtgcc gttcgggctg ttgtggggca gcttcttgaa cgtggtgatc tatcgcttgc
    15901 cgcgtgacat gagcgtggtg agacctggct cgcgctgccc ggcgtgtggc acgccgatcc
    15961 ggccttggaa caacgtgccg gtcctgagct acttgttcct gcgcgggcgc gccagctgtt
    16021 gtcaggctcc tatctcaccg cgctatccgc tggtcgagct gagcggtggg cttttggctt
    16081 gggcagtctt gcgctgcatc gtgttcgagt tgccgggcgg cgcaggcttg gggcaggcac
    16141 tcgggctgtt cgggttgtat ctcgggctc
//
LOCUS       DASTKU010000313         4201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_952571, whole genome shotgun sequence.
ACCESSION   DASTKU010000313 DASTKU010000000
VERSION     DASTKU010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952571"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VFQ61_11225"
     CDS             <1..434
                     /locus_tag="VFQ61_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955070.1"
                     /translation="GGKSSSVAGSGGAGASSGGAVTTYIEPACPDVPASPPAFECDPL
                     SADDALQCDEGFACYPYVTHPFGDGCGTQLVGSMCMGIGSGEQGDNCGGSLGCAPGFI
                     CVVGSHPGRRCAQLCRLDGTANCPAGLICSETDVEPFGVCS"
     gene            447..2006
                     /locus_tag="VFQ61_11230"
     CDS             447..2006
                     /locus_tag="VFQ61_11230"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HET9955071.1"
                     /translation="MRFLGWMALWIGCAACGSRTQLDGAASASGGGTPNPMGCEADAE
                     CGQSDACQTWRCVSRSCQAVQTKCDDGDACTEDRCDAQAGCSHTPLTEDSDGDGHRSP
                     RAGYAASMPGACGDDCDDTSALAHPGRVEVCDGVDNDCNGVVDDGAEYVATSKPPVRV
                     SSNVYQRAARGGLAALDDEFAFTYTGFDGSAHGVLKRVTSGGAALSEADVTRINADSY
                     AGPLVWSGAALATAWEDARQAQNYEIYFARFDRQGQKLGPDLRVTNADSFSLNVTLRW
                     NQSEYVLAWDDRRGDEFAFGTDPARIFGQRIAADGRLLGENVVLIEDEAGAQSPALAV
                     SPTRIGLAYTSFTDTSYVGFRVFDSRFEAVGEVTAGFAPDVESPSVYWVGDRFVVLWS
                     TSRALEPGNAIWAASFDSEGRMLAKPQPITQDATYARSPAALSLGDRLLLVWADDHDG
                     NYELYAQTVDRNLKVLTRRARLTRTEAPTLSPILAPSPNGQVAVLFDDWSSNNRQVYF
                     TSLECKPVGLR"
     gene            complement(2108..3043)
                     /locus_tag="VFQ61_11235"
     CDS             complement(2108..3043)
                     /locus_tag="VFQ61_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HET9955072.1"
                     /translation="MLDQLPREVSIYEVSPRDGLQNEAKPIPVEGKRELVLALVAAGL
                     HRVELTSFVSPRWVPQLADAEELARSLPPPEGVTFSALCPNAKGLERALSVGFKEIAV
                     FLSASETHNLKNTHKSIAQSLDTFAEVVPPALAANMRVRAYVSTVWGCPYEGDVEVSR
                     SLALTQQLLELGCYQVSLGDTIGVGTPLQTRSILRSFLSEVPSERLALHFHDTRGTAL
                     ANALIGLDLGIRDFDSSVAGVGGCPYAPGAAGNLATEDLVYMLEGMGVKTGVDLDRLI
                     EAGRVAERVIGRTLPGKVHQAGPRALRPARSLAAR"
     gene            complement(3161..>4201)
                     /locus_tag="VFQ61_11240"
     CDS             complement(3161..>4201)
                     /locus_tag="VFQ61_11240"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET9955073.1"
                     /translation="AISAQKRPEDRVADALSQVRGAYSLLCLTPHSMIAARDPQGFRP
                     LCLGQMPGQGAYAIASEPSSFDLIGAEYLRDIEPGELVIIDRDGIRSLRPFADSRRST
                     CIFEFVYFARPDSFIDGISVYEARKNLGRALAAQHPVQADVVIPVPDSGVPATIGYSE
                     ASGIRFELGLMRSHYVGRTFIEPQQSIRHFGVRLKLNPISSVLRGKRVIVVDDSIVRG
                     TTSRKIVKMLRAAGAAEVHMRISSPPSKWPCYYGIDTPTRAELIASTHSVEEINQYLT
                     SDSLGYLSLEGMVQSVLKTERRVPNSSRALPVLQSKPMNQHSFCSACWTGEYPVEFSP
                     PPRQRQMRLLDI"
BASE COUNT          700 a         1265 c         1455 g          781 t
ORIGIN      
        1 ccggcggaaa gtcctcgagc gtcgccggga gcggaggcgc tggggcgtcc agcggtggtg
       61 cggtgacgac ctatatcgag cctgcttgcc ccgatgtgcc tgcttcaccc ccggcattcg
      121 agtgtgaccc gctgagcgca gacgatgcct tgcaatgtga tgagggtttc gcgtgctacc
      181 cgtacgtgac gcatccattc ggagatggct gtggcactca gctggtgggc agcatgtgca
      241 tgggcattgg atccggcgag cagggagaca actgcggcgg tagcttgggc tgcgcaccgg
      301 gcttcatttg cgtggtgggc tcccatccgg gccggcgttg cgctcagctg tgccggttgg
      361 acgggaccgc gaattgcccc gccgggctca tctgctccga aaccgacgtc gaacctttcg
      421 gggtctgtag ctaaggccct tttcaaatgc gttttctcgg ttggatggct ttgtggatcg
      481 gctgcgccgc ttgtgggagc agaacgcagc tggatggcgc ggcatcggcg tctggggggg
      541 gcacgccaaa ccccatggga tgtgaagcgg acgccgaatg tggccagagc gacgcctgcc
      601 aaacctggcg ttgtgtctcg cgcagttgcc aagcggtaca gaccaagtgc gacgatgggg
      661 atgcttgcac ggaggaccgc tgtgatgccc aagctggctg ctcacacacg ccgctcacgg
      721 aggactcgga tggcgacggc caccgcagcc cgcgcgcagg ctacgccgcg agcatgcccg
      781 gcgcgtgcgg tgatgattgc gacgatacga gtgcgctcgc tcacccgggc cgggtcgagg
      841 tttgcgacgg agtggacaac gactgtaacg gtgtagtcga cgacggcgcc gagtacgttg
      901 ccacgagcaa gccgcccgtg cgcgtgtctt ccaacgtgta tcagcgtgcg gcgcgcggcg
      961 gcttggcggc gttggacgac gagttcgcct tcacgtacac gggcttcgat ggctccgcgc
     1021 atggagtgct gaagcgcgtg acttcgggcg gagccgcgct ctcggaggcc gatgttacgc
     1081 gcatcaacgc cgattcgtac gcggggccat tggtgtggtc cggagcggcg ctggcgaccg
     1141 cctgggagga tgctcgtcag gcccagaatt acgagattta cttcgcgcgc ttcgatcgtc
     1201 agggccagaa gctcgggccc gacttgcgcg tgaccaatgc ggatagcttt tcactcaatg
     1261 taacgttgcg ttggaaccag tccgaatacg ttttggcgtg ggatgatcgg cgcggagacg
     1321 agttcgcgtt tggcacggat ccggcgcgga tctttggcca gcggattgcg gccgatgggc
     1381 ggttgctggg agaaaatgtg gttctgatcg aggacgaagc gggcgcacag agccctgctt
     1441 tggcggtttc tccgactcgg attgggctcg cgtacacatc gtttacggac accagctacg
     1501 ttggctttcg ggtgttcgac tcacgcttcg aggcggtcgg cgaagtcacg gctggctttg
     1561 cacccgacgt ggagagcccg tcggtgtatt gggtggggga ccgtttcgtg gtgctatgga
     1621 gcacgtcacg ggcgctcgag ccgggcaatg caatctgggc agcgagcttc gactcggaag
     1681 gccgcatgtt ggcgaagccg cagcctatta cgcaggatgc gacgtacgca cgctcacccg
     1741 cggccctgtc gctcggggat cggttgttgc tcgtttgggc ggacgatcac gacggcaact
     1801 acgagctata cgcgcagacc gtggatcgga acctgaaggt gttgacgcgg cgcgcgcgct
     1861 tgacccgcac ggaggcgccg acgctctctc cgattctcgc gccgagcccc aacgggcaag
     1921 tggctgtgtt gttcgatgat tggagcagca acaaccggca agtgtacttc acgtcgctcg
     1981 agtgcaagcc cgtcggtttg cgttgagcgc gccggtaagc tgcgctggat tacttgcgcg
     2041 cagctattaa ttgcgcgcaa ctaggctgcg ctggtgaatt agcgcgcagc taggctgcgc
     2101 tggtgaatta gcgcgcagct aggctgcgcg ctgggcgcag ggctcgcggt ccggcttggt
     2161 gcaccttgcc cggtaaggtg cgtccgatca cacgctcagc gacgcggccc gcctcgatta
     2221 agcgatctag atcgacgccg gtcttaacgc ccatgccttc gagcatgtaa accagatctt
     2281 ccgtggccag gttgccggcc gcaccgggcg cgtaggggca gccgcccact ccggcgacgg
     2341 aagagtcgaa atcgcgaatg ccgagatcga ggccaatcag ggcgttggca agcgcagtcc
     2401 cgcgcgtgtc gtgaaagtga agcgccaagc gctcggacgg cacctcactc aagaagctcc
     2461 gcaggatgct gcgggtctgc agcggggtgc cgacgccaat cgtgtccccg agagaaactt
     2521 ggtagcagcc gagttcgagc agctgctggg tgagggcgag tgaacgcgac acctcgacgt
     2581 ccccttcgta cgggcagccc caaacggtcg acacatacgc gcgcacgcgc atgttcgcag
     2641 ccaacgcggg cggcaccact tcggcaaagg tgtccagcga ttgcgcgata ctcttgtgcg
     2701 tgttcttgag gttgtgggtc tcggacgccg aaaggaacac ggcgatttct ttgaacccga
     2761 cgctgagcgc ccgctcgagg cctttggcgt tggggcacag cgcgctgaac gtcacccctt
     2821 cgggtggtgg tagcgagcgg gcgagctcct ctgcgtccgc gagttggggt acccagcgcg
     2881 gagacacgaa agacgtgagc tcgacccggt gtagccccgc cgcaaccaag gccaacacga
     2941 gctctcgctt gccttcgaca gggatgggct tggcttcgtt ttgcagacca tcgcgcgggg
     3001 agacctcgta gatggatact tcgcgcggta gctggtccaa cacggcccgt ctaagctagc
     3061 acgccaggcc caagtggagg cgggcgccgg cttgggcgcc cccctcacgg gcctgatctg
     3121 ctcgcccccc tctcccgcgg acgggcccca gctcctcgag ctagatgtcg agcaggcgca
     3181 tctggcgctg gcgtggcggt ggggagaact cgaccggata ctcgcccgtc caacacgcgc
     3241 tacagaacga gtgctggttc atcggtttgc tctgcagcac gggcagcgcg cggctgctgt
     3301 tcggaacgcg gcgctcggtc ttgagtacgg actggaccat gccttcgagc gacaagtagc
     3361 cgaggctatc gctggtcagg tattggttga tttcttccac gctgtgggtc gaggcgatca
     3421 gctcggcgcg ggtgggcgta tcgatgccgt agtagcaagg ccacttggag ggcgggctag
     3481 agatgcgcat atgcacctcg gccgcgcccg ccgcacgcag catcttcacg atcttgcggc
     3541 tggtggtgcc acgcacgatg ctgtcgtcca cgacgatcac tcgcttgccc cgcagcacgc
     3601 tgctgattgg gttgagcttc aagcgcacgc cgaagtggcg gatgctctgt tgcggctcga
     3661 tgaaggttcg tccgacgtaa tggctgcgca tcaagccgag ctcgaagcga atgccggacg
     3721 cctccgagta gccgatggtc gcgggcacgc ccgaatccgg caccgggatg accacgtcgg
     3781 cttgcaccgg gtgttgtgcg gccagggccc ggcccaggtt tttgcgagct tcgtagacgc
     3841 tgatcccgtc gatgaaagag tccggtcgtg cgaaatacac gaactcgaaa atacaggtgc
     3901 tgcgccgcga gtcggcgaac ggccgtaggc tgcggatgcc gtcacgatcg atgatcacca
     3961 gctcgcccgg ctcgatgtcg cgcaggtact cggccccgat caggtcgaag ctcgacggct
     4021 cgctggcgat cgcgtacgcg ccctgccccg gcatctgccc caggcacaag gggcggaagc
     4081 cttgcgggtc tcgcgctgcg atcatcgagt gtggcgtgag gcacagcagc gagtatgcgc
     4141 cgcgtacttg gctgagcgcg tccgccaccc gatcttcggg ccgcttctgc gcgctgatgg
     4201 c
//
LOCUS       DASTKU010000314         7033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1622848, whole genome shotgun sequence.
ACCESSION   DASTKU010000314 DASTKU010000000
VERSION     DASTKU010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622848"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..1033
                     /locus_tag="VFQ61_11245"
     CDS             449..1033
                     /locus_tag="VFQ61_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9955074.1"
                     /translation="MSQYADGDDRAFAVVFEILGPRLLRFLRRLSGSDALAQDLLQDT
                     LLRIHQARGSFRPGASVLPWAFAIARNVFLDSARARKRSPIKSHADDADTSPEPVVSA
                     EAESHVLAREAARVVERALMAMSSVRREAFVLVRFEGLSIVDAAEVLGTTPNAVKLRA
                     FQAYEVIREELARLGGTARPLAGSAARASSGSAP"
     gene            1095..1853
                     /locus_tag="VFQ61_11250"
     CDS             1095..1853
                     /locus_tag="VFQ61_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955075.1"
                     /translation="MSDSDSDFDFDPRLLESIPDPLGPRAGSSASMRFEQIGAELPVG
                     IQPALQALPTGSARRTRTELQRARWIAVCGSLLWLGMQLGLSGIRGDMSRVPWGYTLA
                     LVGLPCVAGIGCIAVALAPGRLGLGLRTGLLMMLALLCPAGFLLVGGLQPLPYPEVPL
                     GNFQFGLLCFNVAMGWTLLPLVLAAYALKNSFVGRAGYRSALVGGGIGLIVASASALR
                     CPLSAVSHVVFSHGGAVIASALLGAYALSRVTRV"
     gene            2018..2650
                     /locus_tag="VFQ61_11255"
     CDS             2018..2650
                     /locus_tag="VFQ61_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9955076.1"
                     /translation="MSESSFGARSSALARGASSVRSPMPASVAPSALDTPDEQLALSY
                     IVHDDKQAFRALYERLYPRLLRIAQRRVRCSALAADLVQQAFLNAHAARERFEPGSAY
                     GPWITRILMNLLLDQYRAGRRRVVVDLDPQELPAVEHATSAERLQDIQRARRALERLR
                     PRQRQVLEMHWLEERSFPEVAARLGERVVTVKVRAHRACSELRRELDRVA"
     gene            complement(2666..3403)
                     /locus_tag="VFQ61_11260"
     CDS             complement(2666..3403)
                     /locus_tag="VFQ61_11260"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HET9955077.1"
                     /translation="MTGPDPSTSHPDLFEIPDPLASGASPPLPAVHLPVERPTRRERQ
                     RSRHYAFVFGAAWLVLDLTWFGLRGDLNRLPVWYWLATVASPVLAAVISVVVALAPGA
                     RGLGAKGGLLATIAILPSAALFFSALFLTSHALTPELASNADPAGQGSVIQGTWVDAA
                     YCFNVGLAFAGPPALAALLALRRSFVVNARWRAALIGSAAGLCAAALLNLHCSVVGPW
                     HVAAAHTGATLVFALLGAFAFARYLRA"
     gene            complement(3530..5464)
                     /locus_tag="VFQ61_11265"
     CDS             complement(3530..5464)
                     /locus_tag="VFQ61_11265"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET9955078.1"
                     /translation="MSEFAIAVNDSNEWLEAYGFGGFASGTTGRIRTRRYHALLLAAA
                     SPPTDRYALVQGFVGWVASPRGEEEIWPQRYAGGFETGLQTRSVIYRHDPWPSWRIEL
                     NSGRILQVELFVPRGTPAVVLRFELEGDVADTKLRLRPLLSGRGFHATHHENPGFCFE
                     PEAVGNALRFKPYPGVPAVLSLSNGRFEFSPDWYRKFTYIEEDARGLAPTEDLASPGV
                     LEFDLSQGPADWILKADLPLGIDVRSRAASEFANEVRSLELQRRLAHTERLDRSAEAF
                     LVKRGPGRSLIAGYPWFGDWGRDTFIALRGLCLARGDLDTAAAILLEWAGVVSRGMLP
                     NRFTESATDAPEYNSVDAALWYVLAATELLDAAKGECLSDQDRKRLERAILEIVSGYS
                     AGTRFGIRCTGDGLLAAGTPGLQLTWMDAKVGDWVVTPRIGKPVEIQALWISALQSAS
                     RLNSEYARLESRARHHFEALFWNEEQGCLYDVVDVDHESGRVDQAFRPNQIFAAGGLP
                     VDLLGPARARRVVNAVEQKLWTPLGLRSLAPHEPGYVPRYRGGVAERDGAYHQGTAWP
                     WLIGPFVEAWLKTRPATPASRAEAKQRFLLPLQEHLAHAGLDHVSEIADAEAPHVPRG
                     CPFQAWSLGEMIRLQRLLSS"
     gene            5710..>7033
                     /locus_tag="VFQ61_11270"
     CDS             5710..>7033
                     /locus_tag="VFQ61_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HET9955079.1"
                     /translation="MNKRGLSFGAWFAVSVGASALLSGCSSGSGTPGGGGAAPIGGSP
                     SSGGNASSGGASNSNAGTGAVQPGAGNGGSTAQGGQTAGGGVGNAGVGNAGVGNAGAG
                     SPPGGTAGDWKVGLTQGPNGLIPWIVVDQFGYRAQDPKVALLRDPRQGYDAATDFTPG
                     ASYALVDAKTGAKVKQGAPQAWNNGAVDPKSGDAVWSFDFSDVTALGTYYVLDEQTGR
                     RSPEFEIGPGVYRNVLRHAMRTFFYQRAGFEKKAEFAGAEWADAASHLQPGQDGETHD
                     WLDKTNAAKVKDLRGGWYDAGDYNKYTSWHARYLITLLRSFVRHPAVFGDDSGIPESG
                     NGTPDVLDEVKFGLDWLARMQNEDGSLLCVQTLGSGSPPSSAKDPSYYGPPTTAATLA
                     GAAAFAYAARVFSARSEASLKQAAEDYVARAKKAWTFADGNPSLTYFNN"
BASE COUNT         1293 a         2260 c         2221 g         1259 t
ORIGIN      
        1 catcccggcc accttcattc tcattcccga ggctcagcac ggtgcgatgg gcccgacgcc
       61 agagcaaacc atgggtcaag ctttggattg gctggcgcag aatagaaagc ctctcgttca
      121 gcaagcagcc tctgccccca gctcggggta acagcgtcag cttcggggct tgacggatag
      181 gactccgttc ctgcgggccc gccgtaccac ttttacttcc gcaaagaggc gcgttccgcc
      241 tgaacgagtc tttcagcgga aacctgcgtt gcgggccctc ggctgaacgc tcgacctgag
      301 cgccaggggg aaatcgaacg ggtgcgtgga ctccggtcag taatgccgca ctaggagtgg
      361 tataccgcgg cgccaggtga gtcgggtgcc tccgcatggc gaacaacccc cgtctgacgc
      421 cgcctctttg gccgcagcag accgtgcaat gtcccagtat gcggacgggg acgatcgcgc
      481 cttcgcggtc gttttcgaaa tcttagggcc gcgtctgttg cgctttctga ggaggctgtc
      541 tggaagcgac gcgctcgccc aagatctttt gcaggatact ttgttgcgaa ttcaccaggc
      601 gaggggaagc ttccggcccg gcgccagcgt gctgccctgg gctttcgcga tcgcccgcaa
      661 tgtatttttg gattcggctc gggctcggaa acggtcccca atcaaatccc atgcggacga
      721 tgcggacact tctcctgagc cggtcgtttc ggcggaggcc gagagtcacg tgctcgctcg
      781 ggaggcggct cgcgtcgtgg aacgggctct gatggcgatg tcgagcgttc ggcgcgaggc
      841 attcgtactg gtccggttcg aaggcttgag catcgtggat gcggccgagg tcctgggtac
      901 cactcccaat gccgtgaagt tacgagcatt tcaggcctac gaggtaatcc gcgaggagct
      961 cgctcgtcta ggaggcactg ctcgcccctt ggccggctcg gccgcgcgag cgagctcggg
     1021 aagcgcccct tgattatttc tgacaccaag ccagccgccg ctcgtacgcg cagtatcccg
     1081 caatgatgtg acctttgtcc gactccgact ccgacttcga cttcgaccct cgtttgctcg
     1141 aaagcattcc tgacccgctc gggccgcgcg ccggaagttc ggcctccatg cgattcgagc
     1201 agatcggcgc agagctgccc gtgggaatcc aaccggcatt gcaggctctg ccgaccggga
     1261 gcgcgcggcg cacgcgcacc gagctccagc gcgcgcggtg gatcgcggtt tgcggcagtc
     1321 tgctgtggct aggaatgcag ctcgggctat ctgggattcg aggcgacatg agtcgggtgc
     1381 cttggggtta caccttggcg ctcgtcggcc tcccgtgcgt ggccgggatt ggttgcatcg
     1441 cggtggccct cgcaccaggc cgcttgggcc tgggccttag aacggggttg ctcatgatgt
     1501 tggcgctgct ttgccctgct gggttcctgc tcgtgggtgg gctccagccg cttccgtacc
     1561 ctgaggttcc gctcggcaac tttcagttcg gactattgtg tttcaacgtc gcgatgggct
     1621 ggacgctgtt gccgttggtc ctcgcggcgt acgctttgaa gaacagcttc gtcggtagag
     1681 cgggatatcg aagtgcactc gtcggcggcg gaattggctt gatcgtcgcg tctgccagcg
     1741 cgctccgctg ccctctttcc gcggtttctc acgtcgtctt cagtcatggt ggcgccgtca
     1801 tcgcgagcgc cctgctcgga gcttacgcgc tgagccgcgt gacccgcgtg tgagttaatc
     1861 agagtccgcg ccggcgcggt cgcgatcatc caaaaaagcg cagacccgct cgattgttta
     1921 acaccgacct gattggcctg cgtagtggtg gctgactgcc accgccggca cctcgattgg
     1981 ggggtgtccc tgctcggtgg actcacagga aaatcgcatg tccgaatctt ctttcggcgc
     2041 gcgcagctct gcgctcgcgc gcggggcctc gtccgttcga agtccgatgc ccgcctccgt
     2101 cgctccaagc gcattagaca cgccggacga gcagctcgcg ctgagctaca tcgtccacga
     2161 cgataaacag gcgtttcgcg cgttgtacga gcgactgtat ccacgcttgc tgcggatcgc
     2221 gcagcgtaga gtgcgctgtt ccgcgcttgc cgccgatcta gtgcagcaag cgtttctgaa
     2281 cgcgcatgcc gcgcgcgagc gcttcgagcc gggcagtgca tacggcccat ggattacccg
     2341 tatcctcatg aatctgctct tggatcagta ccgcgcgggg cgacgccgcg tagtggtgga
     2401 cttggatcct caggagctgc cggcggtaga acacgctact tccgccgagc gtttacaaga
     2461 cattcaacgc gcacgccggg cgctcgagcg gctgcgaccg cggcaacgcc aagtactcga
     2521 gatgcactgg ttggaggaac gctcatttcc agaagtggcg gcccgcttgg gggagcgcgt
     2581 ggtgacggtc aaagtacgcg cccaccgcgc ttgttcggag ttgcgtcgcg aactcgatcg
     2641 cgtggcttga gccgattacg agcgctcagg cgcgcaggta gcgagcgaac gcgaacgcgc
     2701 cgagcaacgc gaataccagc gtggcgcccg tatgagcggc cgcgacgtgc cagggcccga
     2761 ctacgctaca atgcagattc aagagcgccg ctgcgcacaa gcccgcggca gagccaatca
     2821 acgccgcgcg ccagcgcgcg ttgaccacga agctgcgacg gagggcgagc agcgccgcga
     2881 gcgcaggcgg accggcgaag gccaacccga cgttgaaaca atacgcggca tccacccaag
     2941 ttccctggat cacgctgccc tgcccggcgg gatccgcgtt gctcgccagc tcgggagtga
     3001 gggcatgact ggtgagaaac agtgccgaga agaacaacgc ggcactgggc aggatcgcga
     3061 tcgtcgccag tagcccgccc ttcgccccca ggccccgtgc gcccggcgcg agcgcaacca
     3121 ccaccgagat caccgcagcg aggaccggcg aggcgaccgt cgccagccaa taccagaccg
     3181 gaagacgatt caaatcgccg cgcagcccga accacgtcag atcgagcact agccacgctg
     3241 ccccaaacac gaaggcatag tggcgcgagc gttggcgctc gcgccgcgtc ggacgctcca
     3301 cgggcagatg cacagccggc aaaggcgggc tcgcgccgct agcgagtgga tccgggattt
     3361 cgaacaaatc cggatgcgag gtcgatggat cggggccagt catcgtgccg tgtcagtgac
     3421 gattataggc gccgtgcgcg attgcgcgag acctatcctt acgcgcgaca gcggcgggtg
     3481 ctcgaaccta gttggccatg aaaccgctct tggcacgtgc tcccgcgatt cagctcgaaa
     3541 gtagccgctg cagccgaatc atctccccga gcgaccaggc ctgaaagggg cagccgcgtg
     3601 gcacgtgcgg agcctctgca tcggcaattt cgctcacgtg gtcgagaccg gcgtgggcga
     3661 ggtgctcctg aagaggcaga aggaagcgtt gctttgcctc ggctcgagag gctggcgtgg
     3721 ccggccgcgt cttgagccaa gcttcgacga acgggccaat cagccaaggc cacgccgtcc
     3781 cctgatgata ggctccgtcg cgttcggcga cgcccccgcg gtaacgcggc acatagcctg
     3841 gttcatgagg cgcgagcgac cgaaggccga ggggtgtcca caatttttgc tcgactgcgt
     3901 tcaccacgcg ccgcgcccgg gcaggaccaa gcaagtccac gggcaggcca cccgccgcga
     3961 agatctggtt cggtctaaac gcctggtcta ctcgacccga ctcgtgatcg acgtccacga
     4021 catcgtaaag acagccctgc tcttcgttcc agaacagcgc ttcgaagtgg tgacgcgctc
     4081 ggctctcgag cctcgcatat tcagagttca atcgggaagc cgactgcaaa gcggaaatcc
     4141 agagcgcctg aatctccacg ggcttgccaa tgcgcggtgt gaccacccaa tcaccgactt
     4201 tggcgtccat ccacgtcagc tgcaggcccg gagtacccgc cgcgagcaac ccgtctcccg
     4261 tgcatcgaat tccaaatcga gttcctgcgg agtatccact gacgatctcc aagatcgcac
     4321 gctccagccg cttgcgatcc tggtccgaca aacattcccc tttcgctgcg tccagcaact
     4381 cggtggcggc aagcacgtac cacagcgccg catcgacgga attgtactcg ggcgcgtcgg
     4441 tcgcgctctc cgtgaagcga ttcggcaaca tgccgcgtga cacgactcct gcccactcca
     4501 agagaatagc ggcggccgta tcgagatcac ctcgcgcgag acacaacccc cttagcgcga
     4561 tgaacgtgtc acggccccag tctccaaacc aaggataacc agcaatcagg ctgcgacccg
     4621 ggccccgctt caccaggaac gcctcggcag agcgatcgag gcgttccgtg tgggcgagac
     4681 ggcgctgcaa ctcgagcgag cgcacctcat tcgcgaactc gctcgcagcg cgggatcgaa
     4741 cgtcgatgcc caggggtaga tccgccttca aaatccaatc cgcgggacct tggctcagat
     4801 cgaactccaa cacacccggg gacgcgagat cttccgttgg cgccaaccca cgcgcgtcct
     4861 cctcgatgta agtgaacttt cgataccaat cgggcgagaa ttcgaatcga ccgttgctga
     4921 ggctcagcac cgctggaact cccgggtacg gcttgaatcg cagcgcgttc ccgaccgctt
     4981 ccggttcgaa gcagaatcct ggattctcgt ggtgcgtggc atgaaagcca cggccggaga
     5041 gcagcggtcg caaacgcaac ttcgtgtcgg ccacatcacc ctcgagttcg aagcgcagaa
     5101 cgaccgcggg cgtgccccgt ggtacgaaca gctctacttg gagaattcgg ccagaattca
     5161 gctctatgcg ccagctcggc caagggtcat gccggtagat gactgatcgg gtttgcaacc
     5221 cagtctcgaa tccccccgcg tagcgttgtg gccaaatctc ctcctcaccc ctagggcttg
     5281 ccacccaacc cacgaagcct tgtaccagcg cgtaccggtc ggttgggggc gatgccgcgg
     5341 ccaagagcag tgcatgatag cgacgcgttc taatccgccc ggtcgttccg ctcgcgaaac
     5401 cgccgaaacc ataggcttcg agccactcgt tagagtcatt gaccgcgatc gcaaattcgc
     5461 tcattgctcc gtattcctga cagccattct gtcagaatcc actcacaccg cgtagtcgga
     5521 cacgggcccg cagtggacac gccccgacta gacattccga tcatgccgat gcaccaccgc
     5581 cgaggcccgg tgcggcgtcg caatcactcc tgtcatgtgg cagggcacgc caatggggcg
     5641 gctcgatcca gatcgattcg ctgctaaggt gggcctgcgc ttctcgagcg ctcgccaatt
     5701 agtgacgaca tgaacaaacg cggattgagt tttggtgcgt ggttcgcagt aagcgtgggc
     5761 gcgagtgcgc tcctcagcgg gtgcagctcc ggtagcggca caccgggtgg tggcggagcg
     5821 gcgccgatcg gtggtagccc ctcgagtggc ggtaacgcta gctctggagg ggcttccaac
     5881 tccaatgctg gaactggcgc cgtgcagcct ggtgccggca acggaggctc cactgcgcag
     5941 ggtgggcaga cggctggcgg gggcgttggc aatgctgggg tcggcaatgc tggcgtcggc
     6001 aatgccggcg cgggcagtcc ccctggcggc accgcgggcg attggaaggt aggtctcacg
     6061 caagggccga acggcttgat cccttggatc gtcgtcgacc aatttggtta tcgcgcacaa
     6121 gatcccaagg tcgcgctact ccgcgaccct cgacaagggt atgacgcggc cactgatttc
     6181 acgcccggag cgagctacgc cttggtcgac gcgaagaccg gcgccaaggt caaacaggga
     6241 gcaccgcagg cttggaacaa cggcgcggtc gacccgaagt caggggacgc cgtgtggtcc
     6301 ttcgacttct ccgacgtgac ggcgctcggg acttactacg tgctggacga gcagacgggc
     6361 aggcgctcac ccgagttcga gattggcccc ggcgtgtatc gcaacgttct ccggcacgcg
     6421 atgcgcacct tcttctatca gcgcgccggc ttcgagaaga aggcagaatt tgcgggcgcc
     6481 gagtgggcgg atgccgcgag ccacttgcaa cccggtcaag acggggaaac ccatgattgg
     6541 ctcgacaaga ccaacgccgc caaagtcaag gatctgcggg gcggttggta cgacgctggg
     6601 gattacaaca agtacaccag ctggcacgct cgctatctaa tcacgttact gcgcagcttc
     6661 gtgcggcacc cggccgtatt cggggacgac tctggcatcc ccgaatcggg caatggcact
     6721 cccgacgtgc tcgacgaagt gaagttcgga ttggactggc tcgctcgaat gcagaacgag
     6781 gacggctcgc tgctttgcgt acagacgttg ggttcgggga gcccgccatc gagcgccaaa
     6841 gatccgagct attacgggcc acccaccacg gccgcgacgc tggcgggcgc ggctgcgttt
     6901 gcttacgctg cgcgcgtatt cagcgcgcgc tccgaggcca gtctcaagca agcggccgaa
     6961 gattacgtag cccgcgccaa gaaggcttgg accttcgcgg acggcaatcc gtcgctcacc
     7021 tacttcaaca acg
//
LOCUS       DASTKU010000315        12507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1623340, whole genome shotgun sequence.
ACCESSION   DASTKU010000315 DASTKU010000000
VERSION     DASTKU010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12507
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623340"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2632)
                     /locus_tag="VFQ61_11275"
     CDS             complement(<1..2632)
                     /locus_tag="VFQ61_11275"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HET9955080.1"
                     /translation="MSSLKKFKPRYWRSFAELENTPEFQEFVEREFNEPLEHAPPNSP
                     ERRRFMELMGASFALMGAAGCRWQEDKLLPLSRRPEGITPGVPRRFATAMDLAGNAVP
                     LHVTNYDGRPIKVEGNPVHPESLGACFAYHQASVLGLYDPDRSDQVKQAGKASSLNEF
                     EVFAGALFADLRAKRGAGLKIVSERTSSPTFAWLKQRLLTELPAAKWATFEPTAKHYA
                     RAGSKIAFGAALRTHLVPEQARVIVTLDADFVGPSFPQALTNARRFARTRVAEGPMSR
                     LYAIESGFSHAGSLADHRLAFRAEQIKAVAAHLDAEISAKARALPEYGPAQAKPGAPF
                     LEAPQAQKFFAALVKDLLANVGHSLVIAGLDQPPEVHALVHRMNTVLGNVGRTVLYTE
                     DPDADEPDDVDALKALTEGLTGEDTLLILGGNPAFTAPADVPFGSSISKVKNSIHLSL
                     YNDETSHLCSWHVPALHYLESWGDVRGYGGRVSLVQPLIQPIYGGRSLIEMLAFVLGE
                     KPDGRALVRSRFPGPPNDERLFQRALDQGFVDGSDARVVEPTFRGLPSFPFTPRELGT
                     EGAANGSYELVFTPDPKVFDGRFANNGWLQELPGSFTKLGWDNAVLVPFGVAEEIGLT
                     DGHLVDLTLGGRTIRLPGVIVPGQAEGSLRVAVGYGRTHAGRVGGSTAMEVEPVGANA
                     YGIRTSESYHVTKGATLRVVGEGPRLAISQDLYGIDKIGQEGIADRVGELYREGHLKE
                     YREEPEFARHKVHHPPLLSLWVPPVSYEGHKWGMSIDLNKCIGCSACVTACQAENNIP
                     VVGRKPMSMGREMLWLRIDRYYIGDPKTADVGFQPVPCQQCENAPCEQVCPVGATMHS
                     SEGLNDMVYNRCIGTRYCSNN"
     gene            complement(2620..3270)
                     /locus_tag="VFQ61_11280"
     CDS             complement(2620..3270)
                     /locus_tag="VFQ61_11280"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET9955081.1"
                     /translation="MKLFVFPRWANKARQLAGVALGVTPLYLVGLVWYGASPKTIDAG
                     YMPKQPVPYSHALHVGQLGMDCRYCHNTVEKAAVAAIPPTQTCMNCHAKVHTQSPYLA
                     PVRQSYESGGPVEWIKVHDLPDYVYFNHSAHVVRGVGCVSCHGRVDQMEEVFQKEPLS
                     MDWCLSCHRNPEPNLRPPEEVTNMTWKAPGDPVEFGKSLREKNHIAPPTDCSTCHR"
     gene            complement(3976..5442)
                     /locus_tag="VFQ61_11285"
     CDS             complement(3976..5442)
                     /locus_tag="VFQ61_11285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9955082.1"
                     /translation="MTIPAKATAGSAAKKTARATPPAEAAEPGGSPNPGMKEEDDFVD
                     EVVEPVAVRGGGSPTESPAVDTEEYIDGDLGTMTQETSAGTDVDSATDSTLSRYFRDM
                     ATHQVMGPDEELQAAQAVELAEVQLWSALLAYVPVAELILDALERDVAQMNPPEERPE
                     ILQAAELRRLSKTHKKQRSKFHADQQREWSELTESLARAVRLPDSDRVWMSNALRIAR
                     QGVEETVDDEPVRVSLPDTPAYKRYLEKVRQADHIQRHAKNRFVKANLRLVVSIARRY
                     NRGRLPLIDLIQEGNIGLMKAVERFDHTRGYRFSTYASWWIRHAISRALADKGRAVRI
                     PVHMLDTYNRVSRATQTIIARTGREPTIEELEKETGVPRDKLDKVKDLYADTPFSLDR
                     PVGDEDGRRFIDFLVEENALSPYDCLANQKWAEEVQRLLGTLTPIESRIIRWRFGLDN
                     EDELTLKEIGDKYNLSRERIRQLQEQAIGKIRKQMREF"
     gene            complement(7611..7967)
                     /gene="rplT"
                     /locus_tag="VFQ61_11290"
     CDS             complement(7611..7967)
                     /gene="rplT"
                     /locus_tag="VFQ61_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET9955083.1"
                     /translation="MSRVKRGPSPRRRHKRVLEQVEGFWGGRKNHYRQAMHALWKSWQ
                     YAYAGRKLRKRDFRRLWIARINAACRLNGTSYSRFISSLKQAGIELDRKVLADMAVND
                     AGAFRKLTETAAQRPS"
     gene            complement(7985..8182)
                     /gene="rpmI"
                     /locus_tag="VFQ61_11295"
     CDS             complement(7985..8182)
                     /gene="rpmI"
                     /locus_tag="VFQ61_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET9955084.1"
                     /translation="MPKMKTNRAASKRFRLTGGGKIRRPKGGAQHGFIGKSRKRRRRL
                     RDNDIVAQGTERRIKILLPYG"
     gene            8532..11882
                     /gene="carB"
                     /locus_tag="VFQ61_11300"
     CDS             8532..11882
                     /gene="carB"
                     /locus_tag="VFQ61_11300"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET9955085.1"
                     /translation="MPRRNDINRILLIGSGPIVIGQACEFDYSGTQGAKALTSLGYDV
                     VLVNSNPATIMTDPELVRRTYIEPLTVDTVSAIIERERPDAVLPTLGGQTALNLALEL
                     HERGVLSRHGVKLIGAQVDAIRKAEDRLLFKEAMKNIGLECPRSGYATSVDEARKIVE
                     TTGYPVILRPSFTLGGSGGAIVYRPEDLDTKVSWALQQSPTHQVLVEESVLGWKEYEL
                     EVIRDSADNFIVICSIENLDPMGVHTGDSITCAPAMTLTDREYQKLRNAARDVMHEIG
                     VETGGSNVQFAVDPKTGRVLVIEMNPRVSRSSALASKATGYPIAKIAAKLAVGFTLDE
                     LTNDITGTSAAFEPTIDYVVVKWPRFAFEKFPGADPRLGPQMKSVGEAMAIGRTFPEA
                     LQKAARSLETGKTGLVSLKDRVDYRLLASEPEVQRDLTRDAPPAAKPRPTLPPASSDE
                     LGRALTRVVTIPTADRLFYVADALRAGISLEKVNQLTGIDPWFLSQIERLIDQEKKIE
                     AAPQLDAELLLESKRLGFSDAQIADLRHSTQGEVRAQRESLGIRSVFRRVDTCAAEFP
                     AKTPYLYSTYETEDEAEVTARKKVIILGGGPNRIGQGIEFDYCCCHAVFALRELGIET
                     IMVNCNPETVSTDYDTSDRLYFEPLTLEDVLSICKEEASSGELLGVIVQFGGQTPLRL
                     AVPLERAGVRLLGTSADAIDRAEDRERFDALLSKLGLKRPHAGIAKSVEEALRIGREI
                     GFPVLVRPSYVLGGRAMMTCYSDSELEAYVGLAIEAAREEGGSQTLLIDQFLSHAIEV
                     DVDLVGDGTSAVIGGVMQHIEEAGVHSGDSTSVLPPHSLDPDVVAVIESQAKALALEL
                     GVVGLMNVQFAVKDREVYVLEVNPRASRTVPFVAKATGRPLAKIAAKVMVGRSLAELG
                     IGDGALPTHVAVKESVFPFSKFPGVDTILGPEMRSTGEVMGLAMTFPGAFDKSLSASG
                     FALPDRGRAFISVKDEDKAQACHVARRLRNLGFEVMATGGTAQTLERARIPVSRINKV
                     LEGSPHVVDAIREGLIQLVVNTTQGTKAIADSYSIRRNALLANIPYFTTMAAALAAVD
                     ALEFANQAKGQVHVRSIQEWHARSGEGA"
     gene            11953..>12507
                     /locus_tag="VFQ61_11305"
     CDS             11953..>12507
                     /locus_tag="VFQ61_11305"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HET9955086.1"
                     /translation="MEARTQAAGDTEDALDTFQDYELGEAAAAARLAELEGLYRRSHA
                     EPRLAVLLARAWNRVSFEFILDAHEQALELGDKPNAAYHLARARAGFERAMYFADSWL
                     GTKDGRFAELVKVPAEFGPYLSKAFGSDSAGALLFGGVARVGYASTSPDPAHASNVRR
                     VGRMMLERAEILAPSLLPGLADLFI"
BASE COUNT         2339 a         3896 c         3977 g         2295 t
ORIGIN      
        1 agttgttcga gcagtaccgc gtgccgatgc agcggttgta gaccatgtcg ttgaggcctt
       61 cgctcgagtg catggtcgcg cccaccgggc aaacctgctc gcaaggtgcg ttctcgcact
      121 gctggcaggg caccggctgg aacccgacgt ccgccgtctt gggatcaccg atgtagtaac
      181 ggtcgatgcg cagccacagc atctcgcgtc ccatcgacat cggtttgcgc ccgaccacgg
      241 ggatattgtt ctccgcctgg catgccgtga cacaagcgct gcatccaatg cacttgttca
      301 ggtcgatgct cattccccac ttgtgccctt cgtaggacac cgggggcacc cacaggctga
      361 gcagcggcgg atgatgcacc ttgtggcgcg cgaactcggg ctcctcgcgg tattccttca
      421 agtgcccttc gcggtacagc tcaccaacgc gatcggcgat accttcttga ccgatcttgt
      481 cgatgccgta gaggtcttgg ctgatggcga ggcgcggccc ctcgcccacc acgcgcaagg
      541 tcgcgccttt ggtgacgtga tagctctcgc tcgttcgaat gccgtaggcg ttcgcgccaa
      601 cgggttccac ttccatcgcc gtggaaccac ccacgcgacc ggcgtgggtg cggccgtagc
      661 ccaccgcgac gcgcaggctg ccttcggctt gcccgggaac gatcacgccg ggcaggcgaa
      721 tcgtgcggcc gccgagggtc agatcgacga ggtggccgtc cgtgagccca atttcctcgg
      781 ccacgccgaa aggcaccagg accgcgttgt cccagccgag cttggtgaag ctgccgggca
      841 gctcctggag ccagccgttg ttggcgaagc gaccgtcgaa caccttcggg tctggcgtga
      901 agacgagctc gtagctgccg ttcgcggcgc cctcggtacc cagctcgcgg ggcgtgaacg
      961 ggaacgacgg caagccccgg aaggtcggct cgaccacccg agcatccgag ccgtcgacga
     1021 atccttgatc caacgcgcgc tggaacagcc gctcgtcgtt cggcggccct gggaagcggc
     1081 tacgcaccaa cgcccgaccg tcgggcttct cgcccaggac gaacgccagc atttcgatca
     1141 aactgcgccc gccgtagatc ggctggatca gcggctggac cagagatact cggcccccgt
     1201 agccgcgcac atccccccag ctctccaagt agtggagcgc cgggacgtgc cagctgcaaa
     1261 gatgcgaagt ctcatcgttg tagaggctca ggtgaatgct gttcttcacc ttgctgatcg
     1321 acgatccgaa cgggacgtcc gcgggcgcgg tgaaagcggg attgccgccg agaatcagca
     1381 gcgtgtcctc accggtcaag ccctcggtca acgccttcag ggcgtcgacg tcgtccggct
     1441 cgtccgcgtc gggatcttcc gtgtacagca cggtgcggcc cacgttgccg agcaccgtgt
     1501 tcatgcggtg cacgagcgcg tgcacctctg ggggttgatc caacccggcg atcactagcg
     1561 agtggccgac gttggcgagc aggtctttga ccaacgcggc aaagaacttc tgagcctgcg
     1621 gcgcctccaa gaagggagcc ccgggtttgg cctgagcggg gccgtactcg ggaagggccc
     1681 gagctttcgc gctgatctcg gcgtcgagat gggcggccac ggctttgatc tgctcggcgc
     1741 ggaacgccag gcggtggtcc gcgagcgacc ccgcgtgaga gaagccgctc tcgatcgcat
     1801 agagcctgct catcggcccc tccgcgacgc gcgtgcgcgc gaaacgacgt gcgttggtca
     1861 gcgcctgcgg gaaactcggc cccacgaagt ccgcatccaa cgtgacgatc acgcgagcct
     1921 gctctggaac caggtgcgtg cgcagagcag cgccgaaggc gatcttgcta ccggcgcgcg
     1981 cgtagtgctt ggccgtgggc tcgaaagtcg cccactttgc cgcgggcaac tccgtcaaca
     2041 ggcgctgctt gagccaagcg aacgtcggcg aagaggtgcg ttcggagacg attttcaggc
     2101 ccgcgccgcg cttggcgcgc agatctgcga acaaggcgcc cgcgaacacc tcgaactcat
     2161 tcagagagct cgccttgccc gcttgcttca cctgatcgct gcggtcgggg tcgtagagcc
     2221 caagcacgct cgcttgatga tacgcaaagc aggcgcccag gctctccgga tgcaccgggt
     2281 tgccttccac cttgatcggg cgcccgtcgt agttcgtcac gtgcagcggc accgcgttgc
     2341 cggctaggtc catcgccgtc gcgaaacggc gtggaacccc gggagtgatg ccttccggac
     2401 ggcgcgagag cggcagcagt ttgtcctctt gccaacggca accagcggcg cccatcagcg
     2461 cgaaggacgc acccatcagc tccatgaagc ggcgccgctc cggagaattc gggggcgcgt
     2521 gctcgagagg ttcgttgaac tcacgctcga cgaactcttg aaactccggc gtgttttcga
     2581 gctcggcgaa gcttcgccag tagcggggct tgaacttctt cagcgatgac atgtcgagca
     2641 gtccgtcggg ggagcgatat ggttcttctc acgcagactc ttgccgaatt ccacgggatc
     2701 tccgggtgcc ttccaggtca tgttggtgac ctcttctggc gggcgcagat tcggctcggg
     2761 attgcggtgg cagctcaagc accaatccat gctgagcggc tccttctgga aaacttcttc
     2821 catctgatcc acgcggccat ggcagctcac gcagccaacg ccacggacca cgtgggcgct
     2881 gtgattgaag taaacgtagt cgggcaggtc gtggaccttg atccactcca cggggccccc
     2941 gctctcgtag ctctgccgta ccggcgcgag atacggactc tgcgtgtgca ccttcgcatg
     3001 gcagttcatg caggtctgcg tcggcggaat cgctgccacg gccgcctttt ccacggtgtt
     3061 atggcagtaa cgacaatcca tcccgagttg cccgacgtgc agcgcgtgac tgtacggaac
     3121 cggttgcttc ggcatgtatc cggcgtcgat cgttttgggc gacgccccat accaaaccag
     3181 acccaccaag tagagaggcg tcacgccgag ggcgacaccc gccaattgcc gggccttgtt
     3241 agcccaacgc ggaaaaacga aaagtttcat ggcaaaggac cagaggctcg gaactcgaca
     3301 cgccgcccag cgcatgcgct ggcgcgagac ctatgagttc cccacgctaa atcccttgaa
     3361 cagcaccccg aacgcccatc agccgcggct acgacattta tgtcaggcgc ggggttcagg
     3421 atgggaaggg ggccgtcagg gcgcacgcga ggaagctcgg cgcactttcc gacagctcac
     3481 ggggcttaca gctttacagc tgttttgcgc agcgattacc gccgctctga gctccggtca
     3541 cgggtcggtt ccgtgcgcac agaaaggtac ttctgtttga cgcgcgccct tatgcgcccg
     3601 ctgcccagaa atgtaaatgg ttagtcgcga aaccagcgca agtccgcgta tctaatacag
     3661 attacgttct gattagacct ctgatccggt caaccggacc gaacttttac gggcgacaac
     3721 ctacagggaa gcgcaaagat ccagcgagca cgagcccccg tgatttctcg agttggatct
     3781 gctcgagtag atgctcgcga attctccgac gatggacgaa acccgagcca ccactgccgt
     3841 ggagctcggg cctcgatttc gcgccctttt ggcgctcggt ttgatttcgt ctctcgctca
     3901 gcgtgcgtcg cgtaacgttc cgtaaccgat ttggtttatc tcgcgactcg ctcgagttgc
     3961 tggtcgtatt gttgctcaga actcacgcat ctgcttgcga atcttaccga tcgcctgctc
     4021 ctgaagctga cggatacgct cccgagacag attgtacttg tcaccaatct ccttgagcgt
     4081 gagctcgtcc tcgttatcga gaccgaagcg ccagcggatg attcgggact cgatcggggt
     4141 gagcgtgccg agcaggcgtt ggacttcctc cgcccacttt tgattggcca agcaatcgta
     4201 cggagacaag gcgttttcct cgaccaggaa gtcgatgaat cggcgcccgt cctcgtcgcc
     4261 cactgggcga tcgagagaga acggcgtatc cgcatacagg tccttgacct tgtcgagctt
     4321 gtcgcgcggg acccccgtct ccttctcgag ctcctcgatg gtgggctctc gaccggttcg
     4381 ggcgatgatg gtctgggtcg cgcgggatac gcggttgtac gtatccagca tatgaaccgg
     4441 aatgcgcacc gcgcggccct tgtccgccag ggctcggctg attgcgtggc ggatccacca
     4501 cgaagcgtag gtcgagaagc ggtaaccacg cgtgtgatcg aagcgctcca cggctttcat
     4561 caagccgatg ttgccctcct gaatcaaatc gatgagcggc aaacgaccgc ggttgtagcg
     4621 acgcgcgatg ctcacgacca aacgcaggtt ggctttgacg aagcggttct tggcgtggcg
     4681 ctggatgtga tcggcctgac gcaccttctc cagataccgc ttgtatgccg gcgtatccgg
     4741 tagagacacg cgcaccggct cgtcgtctac ggtctcttcc acgccttggc gagcaatcct
     4801 caaggcattg ctcatccaga cccgatccga gtccggcaac cggacggcgc gagccaggga
     4861 ctccgtcagc tcactccatt cgcgttgctg atcggcgtga aacttggagc gctgcttctt
     4921 gtgcgtcttc gacaggcgcc gcagctccgc ggcctgcagg atctccggac gctcctcggg
     4981 cgggttcatc tgcgccacgt ctcgctcgag cgcgtcgagg atgagttccg ctacggggac
     5041 gtacgcaagc agggccgacc agagctgaac ttctgcgagc tcgactgctt gagcggcctg
     5101 gagttcctcg tccggtccca tcacttgatg ggtggccatg tcccggaagt atcgagacag
     5161 cgtggaatcc gtagccgaat cgacgtcggt tcctgcgctc gtctcctgag tcatcgtgcc
     5221 gagatcaccg tcgatgtact cttcggtgtc gacggccgga gattccgtcg ggcttcctcc
     5281 gccccgtact gctacgggct cgacgacttc atcgacgaaa tcgtcttctt ccttcatccc
     5341 ggggttgggc gaacctcccg gctctgcagc ttccgcaggc ggggtggctc gggcagtctt
     5401 cttcgcagca gatccagcgg tggctttggc aggaatggtc actgacgctt cctcctcaga
     5461 ctgattctta agcaaaccac gtgccttcgt cttggacggc gacgtggcgg gaacgcccac
     5521 ggtgcgtgag acctcgtcgt caacggtatg cttggcgtcg cccgcgggct tcggctcgcc
     5581 ggtgtgcttt ggctcgaccg cttgctcggc ccccaaagcg tgcttcgctt cggcgccgtg
     5641 cttcggttca tgtgccgctt tgctccctcc ggccgccttc gatttggacg aacccccaga
     5701 tttgcgacgc ggggtgctgg taactgaggg ggtgcgagca gtcattggaa aaactctggt
     5761 gagggttacg cctgcagaag aagactcaga agggatgcct gcgctgcgtt ggctgctaaa
     5821 gtcacgggcg cgaccccgaa acttgggtta gcaacacacg gaacggcagg gatgttccct
     5881 cgcgatagcg aggatgagcg atctcgtgcg gagaaagctc gtaccaacaa gccgcggaac
     5941 ggaactgatc gcgaacagga acagaagcgc cggaccaaac gaggttcagc gcgcaatcac
     6001 tcgagggccg ggcccgagca cgggacgggg aacggcgagg ctaaagagaa aggcagaaac
     6061 ggccgggtag ctgcgcggtc gcgcgaatag agggcgaata gagggccggc aaacgcaggg
     6121 tcgaaagaga cacacttggt cagagggaca gatcgagcga atcacagacg gaaaagaaag
     6181 ctcggagaag aatgagagcg tgttttcgtc tctgtagacg accgagttag acgaacgatt
     6241 gccccaacat atgtgttcga cacgcaataa agaggggacc gttcgacgag gctccgtaaa
     6301 aaagacagac tggggaaaaa cgctactggg aaactgccgc aacatagaac cagaggagca
     6361 aaggtgcggc cgcggttacg ggaatgcgca gagcccgcta agatagcggt cgttcgaagt
     6421 caactgttcg cggaagcgct agagacttcc gacagttctc tagtattgga atttatctct
     6481 cttgttctgg tgtaagtgca ccgctcgtgg gcggcagagg caggattgga gatgccgcca
     6541 tcaacagacg tgctatccga tgggggtacc tctccggtgg gcttggggag ccaccgataa
     6601 cgaacgctcg acagaaaaaa gatcgagaag tcttcgaacc caaactctgc gacctcggcc
     6661 cataacttcg gtccgagtga aagctccgtc aaggtgtcag gagcgtagct tgcgtgttgc
     6721 cgtgagtacg caatcgttgc agactcgcgg catcagcggc acaccctgtg ccgttgcgat
     6781 cagatgatcg gtgtgtggcg tggggtgcga tctggaacgc tgcagataca tattggaacc
     6841 cgagggtggt gcgagaaggc aagactggag caaggccggc cggctactca ggaagtgtgt
     6901 gagcggcttg cggccaagtt cggaggtggg cctgctcgag acaagacgcg ttggatggag
     6961 acggcgcgga ccttggcggg caaaagtcgg cacgaacaga aacaaggccg gaagcgaacg
     7021 agacgccgtt accttgacgc cgagaggcgt ccggtcatgc gtttcccttg agcacgtagc
     7081 gagccaactc cgacggacta cccgtcgcgc cagaccagag atgccttggg ctcacaccag
     7141 ctagtgtctc gaggcccgcc gaagccgtca atgaagcggc cgaatcgagt ttagagcgac
     7201 ttgatgacgc cgacgcggcg agtgccctgg ccgatggcga tccctcgctg ggatgagcac
     7261 gcccaagggc tgccacaaag cgagaacggc gatacgggaa gtctgccgcc cgttgcccgc
     7321 acggaaacca cattcaccgg cgtcttggag ggcaccaccc actgaggaac cgcgcggcac
     7381 cgagctggtc cgcgacgact cacgccataa ttccgctcca aggaactacc gcgagctcgc
     7441 ccgggacacc aagtagggcc tttgcccaac cgcagccaac ttgggtggca catggccaac
     7501 tcgatagccc taaacccgga tcaacgagat tgactcggca ccaaagcgaa agccgcgtgg
     7561 tgcatcacca cgcggcttca catcacgctc gaggtcgcag cgcgtttgct tcagcttggg
     7621 cgctgagctg cagtctcggt cagcttgcgg aacgcgccgg catcgttgac ggccatgtcc
     7681 gccagcacct tgcgatcgag ctcgatcccg gcttgcttga gcgacgaaat gaagcgcgag
     7741 tagctggtgc cgttcaaacg gcaagcggcg ttgatgcggg caatccacag acgacggaag
     7801 tcacgcttgc ggagcttgcg tccggcgtag gcgtactgcc aggacttcca cagcgcatgc
     7861 atggcctggc ggtagtggtt cttccgacct ccccagaacc cttcgacctg ctcgagaacg
     7921 cgcttgtggc gccgtcgggg actaggacct cgtttaacgc gggacatact gttgctcctc
     7981 tatctcagcc gtacgggaga aggatcttga tgcgacgctc ggttccctga gccacgatgt
     8041 cgttgtcacg caaacgacgg cgtcgcttgc gcgacttgcc gatgaatccg tgctgcgcgc
     8101 cacccttggg gcgtcgaatc ttcccgcccc cggttaggcg gaaacgcttg ctggcagcgc
     8161 gattggtttt catcttcggc ataggaaatc cactctcaaa ttctcgagta ccaaattctc
     8221 ggttgacgca tgcccgtacc ctgacgggcc gtccgggagc gcgctcttat gaaggaaccg
     8281 cccgctttcg tcaaggggac ggcgcgcgct tccatacacc agggcttttt gctttgtgaa
     8341 gtccatgttg gagggagggc tcggaaagtc catgacgggt gatcttcccc gagcggccat
     8401 gcggccgccg cgcgctccag cggcacgcct tccttctaat accgtgacag ctgacggcga
     8461 ataagaattg gaagcattgc tgaagatcga gcgcatcggc gacttgtgcg tgtgctaagg
     8521 cgcattcccc gatgccgagg cgaaacgaca tcaaccgaat tctgctcatt ggctcgggtc
     8581 cgatcgtgat cggacaggcc tgtgagttcg actactccgg aacacaggga gccaaagcgc
     8641 taacctcgct tggctacgac gtggtcttgg tgaactcgaa ccccgccacg atcatgactg
     8701 acccggagct cgtgcggcgc acgtatatcg agcccctgac ggtcgacacg gtctcggcca
     8761 ttatcgagcg agagcgaccg gacgcggtgc tcccgacgtt gggcgggcaa actgcgctca
     8821 acctcgcgct cgagctgcac gaacgcggcg tgctctcgcg ccacggcgtg aagctgattg
     8881 gcgcgcaggt cgacgcaatc cggaaggcgg aggatcgcct gctgttcaaa gaggcgatga
     8941 agaacatcgg cctcgagtgc ccgcgctcgg gctacgcaac cagcgtagac gaagcacgca
     9001 agatcgtgga gaccacgggc taccccgtaa tcttgcgacc gagtttcacg ctcggcggca
     9061 gcggcggcgc aatcgtctat cgcccagagg acctggatac gaaggtatca tgggcgctcc
     9121 agcagtcccc gacccatcag gttttggtcg aagagagcgt actcggatgg aaagagtacg
     9181 agctcgaggt gattcgcgac tcggcggaca acttcatcgt gatctgctcg atcgaaaacc
     9241 tcgatccgat gggggtacac acgggcgact cgatcacttg tgccccggcc atgacgctga
     9301 ccgatcgcga gtatcagaag ctgcgcaacg cggcgcgcga cgtgatgcac gaaatcggcg
     9361 tggagaccgg aggctcgaac gtccagttcg ccgtggaccc caagacgggg cgcgtgctgg
     9421 tgatcgagat gaatccgcgc gtttcgcgct ccagcgcgct cgcctccaag gccaccgggt
     9481 atcccatcgc aaagatcgcc gcgaagctcg cggtgggctt cacgttggat gagctcacca
     9541 acgacatcac cggcacgagc gcggcgttcg agcccacgat cgactacgtg gtcgtgaagt
     9601 ggccgcgctt cgcgttcgag aagtttccag gagccgatcc gcggctcggc ccgcagatga
     9661 agagcgtggg cgaagccatg gccatcggcc gcaccttccc cgaggcgctg cagaaggccg
     9721 ctcgctctct ggagacgggc aagaccggtt tggtgagcct caaagatcgg gtggactacc
     9781 gcttgcttgc gagcgagccg gaagtgcagc gcgatctcac gcgtgacgcg ccccctgccg
     9841 ccaagcctcg ccccaccttg cctcccgcga gctcggatga actgggccgt gcgttgacga
     9901 gggtggtcac gatcccgacc gcggatcgac tgttctacgt ggcagacgcg ctgcgcgcgg
     9961 gcatttcgct cgagaaggtg aaccaactga cgggcatcga cccttggttc ctctcgcaga
    10021 ttgagcgcct gattgaccaa gagaagaaga tcgaggcggc gccgcagctc gacgcggagc
    10081 tgctgctgga gagcaagcgc ttgggcttca gcgacgcgca gatcgccgat ctacgccaca
    10141 gcacgcaggg cgaagttcgt gcgcaacgcg agtcgctcgg cattcgttcg gtgttccgcc
    10201 gcgtcgatac ttgcgccgcg gagttccccg cgaagactcc gtacctgtac tcgacctacg
    10261 agacggagga cgaagccgag gtcaccgccc gcaagaaggt gatcatcctc ggtggcggtc
    10321 cgaaccgcat cggccaaggc atcgagttcg attattgctg ctgtcacgcg gtgttcgcgc
    10381 tgcgggagct cgggatcgag accatcatgg tcaattgcaa tcccgagacg gtctcgacgg
    10441 actacgacac ctcggatcgc ctttacttcg agccgctcac gctcgaagac gtgctctcga
    10501 tctgcaagga agaggcaagc tcgggcgagc tgttgggcgt gatcgtgcag ttcggcggtc
    10561 agacaccgct gcggctcgcc gttccgctcg agcgcgcagg ggtgcgtctc ctcggcactt
    10621 cggcggacgc gatcgataga gccgaggatc gcgagcgctt cgacgctctg ctctccaagc
    10681 tggggctgaa gcgcccgcac gccggcattg cgaagagcgt ggaagaagcg ctccggattg
    10741 gtcgcgagat cgggttcccg gttctggtgc gcccctccta cgtgctcggc ggtcgcgcca
    10801 tgatgacctg ctacagcgac agtgagctcg aagcttacgt cgggctggcc atcgaggcgg
    10861 cacgcgaaga gggcgggtca cagaccttgc tcatcgatca gttcttgagc cacgccatcg
    10921 aagtagacgt cgacctggtc ggcgatggca ccagtgcggt gatcggcggg gtcatgcagc
    10981 atatcgaaga agcgggggtc cactccggtg actcgaccag cgtgctgccg ccgcactcgc
    11041 tagaccccga cgtggtcgcc gtgatcgaga gccaagccaa ggccctcgcc ctggagctcg
    11101 gcgtggtggg tctcatgaac gtgcagttcg cggtgaagga ccgcgaggtg tacgtgctcg
    11161 aggtcaatcc gcgcgcgtca cgcacggtgc cgttcgtagc caaggccacg gggcgacccc
    11221 tcgccaagat tgccgccaag gtgatggtgg gtcgcagctt ggccgagcta ggcatcggcg
    11281 acggcgcgct gcccactcac gtggccgtca aagaatcggt cttcccgttc tccaagttcc
    11341 cgggtgtgga cacgatcctc ggccccgaga tgcgctcgac cggcgaagta atgggcttgg
    11401 cgatgacgtt tcccggagcg ttcgacaagt ccctctctgc gtcgggcttt gcgctccccg
    11461 accgggggcg cgccttcatc agcgtaaagg acgaggacaa ggcgcaggct tgtcacgtgg
    11521 cgcgccggct tcgcaacctc ggcttcgagg tgatggccac gggcgggacc gcccaaaccc
    11581 tggagcgcgc gcgcatcccg gtttcgcgca tcaacaaggt gctcgaaggc tcaccgcacg
    11641 tcgtggacgc aatccgagag ggcctgattc agctcgtggt gaacacgacc caaggcacca
    11701 aagccatcgc ggacagctac tcgattcgac gcaacgctct gctcgccaat atcccctact
    11761 tcacgactat ggctgccgca ctcgccgcgg tggatgcact cgagttcgcc aaccaggcga
    11821 agggccaagt gcacgttcga agcatccagg agtggcacgc tcgctccggc gaaggagcct
    11881 agactgcgcg cgtgggcgtt ctcagcgttg ctggcccttt cgggctcgct cggctgcgcc
    11941 gaggcttccc tcatggaggc ccggacgcag gctgctggcg acacggagga cgcactcgat
    12001 acgttccaag actacgagct cggcgaggcc gctgccgcgg cgcgcctggc cgagctcgaa
    12061 ggtctgtatc gtcggtcgca cgccgagccg cggctggcgg tattgctcgc ccgggcttgg
    12121 aaccgcgtca gcttcgagtt cattctcgat gcgcacgagc aagctttgga gctcggagac
    12181 aagccgaacg ccgcgtacca tctggcacgt gcccgcgcag gcttcgagcg cgccatgtac
    12241 ttcgcggatt cgtggctcgg cacgaaggac ggccggttcg cggagctcgt gaaagtgccg
    12301 gccgaattcg gcccctattt gagcaaggcc ttcgggagtg actccgcagg cgcgctgctg
    12361 ttcggcggtg tcgcacgggt gggctacgcc agcaccagcc ccgatcccgc ccacgcctcg
    12421 aacgttcgcc gggttgggcg catgatgctg gaacgagccg agatcttggc gccatctctg
    12481 ctgcccgggc tggcggactt gttcatt
//
LOCUS       DASTKU010000316         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_953312, whole genome shotgun sequence.
ACCESSION   DASTKU010000316 DASTKU010000000
VERSION     DASTKU010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953312"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VFQ61_11310"
     CDS             complement(<1..966)
                     /locus_tag="VFQ61_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET9955087.1"
                     /translation="MSWSSIALVCIPLLPALAGLVPFRRARTASVASVGALALAAAAA
                     AYTFWVAFTSQAPNVARFALSTLPSRPLSIGFMADRPSASLALLITGLGWCVLRFSAR
                     YLQGDPRQAQFLRGVALTLAAVTALCLAGDVVVLLVAWVATSVLLHRLLVYYADRPRA
                     VQAAKTKLVFSRLAELCLVAALLLLVERLGTLELPAMFSRAQQASTADLALPALLLVG
                     CALFKSAQFPFHAWLPDTMEAPTPVSALMHAGIINAGGFLVIRFSPVVGQVPAALNML
                     VVVGTLTAVFGAFVMLTQPAVKRSLAYSTVAQMGFMLLECGLSLFG"
     gene            1040..1930
                     /locus_tag="VFQ61_11315"
     CDS             1040..1930
                     /locus_tag="VFQ61_11315"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9955088.1"
                     /translation="MPGQLNYHHLRYFHAIAKAKSMRQAAERLRLSPPALSAQIKLLE
                     SQLGHRLFERAPSGPTLTEAGRIAFDYAETIFRTGDELEGVMLQGAPQPRRTLRVGAV
                     ATLSRNFLIGAFQPLLARSDVGLTVRSGALSDLLALMQAHEIDVVLSNQPAPRGPHSS
                     CHSQLLAEQPVSLVGPSRWKRRSFRFPEECATVPLALPGLDSNLRAAFDALMDEAGIR
                     PLIAAEVDDMAMLRLLARETDGLTLVPRIVVKDELERGTLIEKRKLRRLKETFYAISP
                     SRRFPNTLVRDLVDHVASRG"
     gene            2198..3631
                     /locus_tag="VFQ61_11320"
     CDS             2198..3631
                     /locus_tag="VFQ61_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET9955089.1"
                     /translation="MQFSQLTSSFCIESSSTQRVRAVASFAGRCVIRQALCLALASLG
                     VTGCSQHDESSPHGSRAPTESYEGTGAKPEESRKILDWKGYTWVVSDGAMAGNMQADP
                     ANVFVKNDEVHLRLVKQPDGSWKGAELRAQGRLGFGTYQWQVDAPLDSFTDAVLGLFN
                     FGRADQIGEEYQNEIDIEFARWGYEQNPNLWWTVWPSVGSTPTHETRSFSLNGGTRST
                     SRFLWSRTKVSTFFLEGYAPLDSEGSQLVTSWTFQPDQQEAIPQQALPLLMNLWCAQK
                     DENTQCALTQDVEVVIHDFQFVPLNETPDEPVPQPPLVSDPIGDPELNLAREATAYAW
                     SANTESQSNDHRVESAGLNDGDSTSGVMLNDAGEDGKALWEAAGLLWSSPRTISSVAF
                     TNGALDSNGNGYFQSSCRLQFTTDGNSWRDSAWNSSPDYPYSAEAGGQTYTFSGPEFQ
                     ALGVRVAGETGDESWSASIAELQAKGK"
BASE COUNT          785 a         1228 c         1220 g          605 t
ORIGIN      
        1 gccaaacagg ctaagtccgc actcgagcag catgaagccc atctgtgcga cggtggagta
       61 agccagcgag cgcttgaccg ccggctgcgt gagcatcacg aaggcaccga acaccgcggt
      121 cagcgtgccg actaccacca gcatgttcag cgccgcgggt acctggccca cgacggggct
      181 gaagcgaatc actaggaacc cgccggcgtt gatgattccc gcgtgcatca gtgcggacac
      241 cggcgtcggc gcctccatcg tgtccgggag ccaagcgtga aagggaaact gcgcggactt
      301 gaacagcgcg caacccacca acaagagcgc cggtaacgcg aggtccgcgg tcgaggcttg
      361 ctgggcgcgt gagaacattg cgggcagctc gagcgtgccg agccgttcga ccagcagcag
      421 cagcgccgcg accaggcaaa gctctgccaa acggctgaaa acgagcttcg tcttcgcagc
      481 ttgtaccgct cggggccgat cggcatagta gacgagcagc cgatgcaaca gcacgctcgt
      541 tgcgacccag gctacgagta gcaccacgac gtcgcccgcc aggcacaggg cggtgaccgc
      601 cgcgagcgtc agggccacgc cccgtaagaa ctgcgcctgc cgcggatcac cctgaaggta
      661 gcgcgccgag aagcgcaaga cgcaccaacc gagcccagtg atgagcagcg cgaggctcgc
      721 cgaaggccga tccgccatga acccgatgga aagcgggcgc gagggcaacg tgctcagcgc
      781 gaagcgcgcc acgttcggtg cttgcgaagt gaaggctacc cagaaggtat aggcggccgc
      841 cgcggcagcg agcgcgagcg cgccaacgct tgcaaccgag gcggtgcggg cgcgccgaaa
      901 tggcaccagc ccggcgaggg cgggcagaag agggatgcac acgagcgcga ttgaggacca
      961 gctcatgagg gggattcata cgcagccggc gcattaagcg cgatgacata gtagttaccc
     1021 gagtgttaga tttatcttca tgcccgggca gctcaactac catcaccttc gctacttcca
     1081 cgccatcgcc aaggccaaga gcatgcgcca agcggctgag cggctgcgac tctctccgcc
     1141 ggcgttgagc gcccaaatca agctgctcga atcgcagctc gggcaccgcc tgttcgagcg
     1201 cgcgccctcc gggccgaccc tcaccgaggc cgggcgcatc gcgttcgact atgccgagac
     1261 catattccgg acgggtgacg agctcgaggg ggtcatgctg caaggcgccc ctcagccgcg
     1321 gcgtacgctg cgagtgggtg cggtcgccac gctgtcgcgc aacttcctga tcggcgcttt
     1381 ccagccgctg ctcgcgcgca gcgatgtcgg cctcacggtg cgctcgggcg cgctttcgga
     1441 cctgctcgcg ctgatgcaag ctcacgagat cgacgtcgtg ctgtcgaacc agcccgcgcc
     1501 ccgcggccct cacagctcgt gccacagcca gctgcttgcc gagcagccag tgagcctcgt
     1561 cggcccaagc cgctggaagc gacgtagctt tcgattcccc gaagagtgcg ccaccgttcc
     1621 gctcgcgctt cccggcctgg atagcaactt gcgcgccgcg ttcgacgcac tgatggacga
     1681 agccggcatc cggccattga tcgcagccga ggtcgacgac atggccatgc tgcgcctgct
     1741 ggcgcgcgaa acggacggct tgaccctggt gccccgcatc gtggtcaagg acgaactcga
     1801 gcgtggcacc ttgatcgaga agcgtaagtt acgccgcctc aaagaaacct tttacgccat
     1861 ctctccgagc cgccgcttcc cgaacacgct cgtgcgcgac ctcgtcgatc acgtcgcctc
     1921 gcgcggatag gggcctcgag cgaaccgagg ctcacccact tcttgcggga cgaacactgg
     1981 gggggtgagg ctcatcgagc cgaacaccat tgaaaccgct cgcgacaacg tcccaaccaa
     2041 acgaacctct cattgaacga atccaaaatc gacaagacgc atgtcgatta gtcagtgcgc
     2101 aaacgctcac ccgaactcca aggcgacacc gagcagcaga ccgctacggt ctcggccgcg
     2161 cagcggcgcg gtgcgctcga tttcgaggag tcatgtcatg caattcagtc agctcaccag
     2221 ctctttctgc atcgaatcgt cgtcaactca acgtgtgcgc gcggtggctt cgttcgcggg
     2281 gcgatgcgtg atccgtcagg ccctctgctt ggcgctcgcc agtctcggcg tcactggctg
     2341 ctcgcagcac gacgaaagct ctccgcacgg ctcgcgggcg cctaccgagt cttatgaagg
     2401 taccggcgca aaacccgaag aatcccggaa gatcttggac tggaagggat acacgtgggt
     2461 ggtgtccgat ggcgcgatgg caggcaacat gcaggcggat ccggccaacg tcttcgtgaa
     2521 gaacgacgaa gtgcacttgc gcctcgtcaa gcagcccgac gggagttgga agggtgccga
     2581 gctgcgcgcg caaggcaggc tcggctttgg gacctatcag tggcaggtgg atgcgccact
     2641 cgattccttc accgacgcgg tgctcggcct gttcaacttt gggcgcgccg accaaatcgg
     2701 cgaggaatac caaaacgaaa tagacatcga gtttgcacga tggggctacg agcagaaccc
     2761 aaacctgtgg tggacggtgt ggccttcggt gggttctacg ccgacgcacg agacgaggag
     2821 cttttcgctg aacggcggca ctcggtcgac ctcgcggttc ctttggagca gaaccaaagt
     2881 cagcacgttc tttttggaag gctacgcgcc cctcgacagc gagggaagtc agctcgtcac
     2941 gtcgtggact ttccaaccgg accagcagga ggccattccg cagcaggcgc tgccgctgct
     3001 catgaacctc tggtgcgctc aaaaggacga gaacacccaa tgcgcgctca ctcaagatgt
     3061 cgaggtagtc atccacgact ttcaattcgt tccgttgaac gagacgccgg acgaaccggt
     3121 cccgcaaccg ccgttggtca gtgacccaat tggcgatccg gaattgaacc ttgcgcgcga
     3181 agccaccgcg tacgcgtggt cggcaaatac ggagagtcaa tcgaatgacc atcgcgtcga
     3241 gagcgcgggt ctgaacgacg gggattcaac gagcggcgtg atgctgaacg atgctggcga
     3301 ggacggcaaa gccttgtggg aagcggcggg gctactctgg tccagcccaa gaacgattag
     3361 cagcgtcgca ttcaccaacg gtgcgctgga cagcaatggc aacgggtact tccaaagcag
     3421 ctgccgcttg cagttcacta ccgacggcaa ctcttggcgc gattcggcgt ggaactcgag
     3481 cccggactat ccttactcgg cggaagcggg aggccaaacc tacacgttca gcgggcctga
     3541 atttcaggcg ctgggagtgc gagtcgccgg cgaaaccgga gacgagtcct ggagcgccag
     3601 catcgctgag ctccaagcca agggaaagtg agttgagttt cgtcgagccg gagcactcac
     3661 gacactggac gctctggccg cggaactcga gccgaacatg ggcccgaacg ctgcgaggac
     3721 tatcgtcgcg cgtcgcgacg catcgctcgc gaagcccagt cagtcactcc cggttcggtg
     3781 atcggggcaa cgacgccgtc ccggttcggt gatcgggaca acgcggcact cgcgaggg
//
LOCUS       DASTKU010000317         4593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1400831, whole genome shotgun sequence.
ACCESSION   DASTKU010000317 DASTKU010000000
VERSION     DASTKU010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400831"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(405..542)
                     /locus_tag="VFQ61_11325"
                     /pseudo
     CDS             complement(405..542)
                     /locus_tag="VFQ61_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            complement(756..1871)
                     /locus_tag="VFQ61_11330"
     CDS             complement(756..1871)
                     /locus_tag="VFQ61_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955090.1"
                     /translation="MKPIARNSISRGLGAVLRLVGPLSITASSWAPFAAIGTVGCSGV
                     ESQPDTSRSRAPRTKAQAITTNCSMDLVGLPCDPDGGGDATECEGLCWIGADRSAQCL
                     PIASAGLSAEDMNGRICGDASGSSCEKSCENGQCVAKNARLGAACRPLGLGNACEGLC
                     TLRSGSPYCELVTACREVGVHGCFLRACELTDNSKTGCVDYPLDAGTRCDDGNACSDQ
                     DTCDGHGQCNSGVESCIGTGGAGAGGQDSGAAGGRNAEAGAAGAVTVAGTTGEEEAGS
                     GPGGAAGQPAIGPAGESHSGGSDSLGGAAGAAGASGSGETKPRTPSNRASQISGGGCQ
                     LHGLRVAAHHSSLLGLLGALALRVARRRRRGSTHIAV"
     gene            complement(1868..3583)
                     /locus_tag="VFQ61_11335"
     CDS             complement(1868..3583)
                     /locus_tag="VFQ61_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9955091.1"
                     /translation="MRRFFSSLVLALSPMLLARAAFAETTTRGFALDRFEPSERGSRW
                     FTLESLDFGLSRRAARTSGFAAGAVFDYARQPLVFRDPEGNRTADYVGHQLFAHLGAS
                     VAVLPNLRLALNLPVALVDSGDGGRIADTQYALSGGAQLGDVRIGVLYRLLRLPTNLL
                     RAGVSLDFGLPTGSRSAFTGDGGVRISTNLHVAGDVSKVSYGLRLGPRWHAQTDNFAG
                     VPFGADLVFAGSLGLRALSDRLLLGPEFFASTVLSDGGDGFFAPNSTPLEVLLGAHYD
                     LESDVRIGVGAGRGLTQALGTPAFRVVASVEWVPGGLSDKAPATPPDTDRDGIADAYD
                     ACPLQPGIASKLLAENGCPPRDTDLDTVPDALDACPAEPGVAANSPRENGCPRKPPIV
                     DQDHDGVLDPEDDCPTEPGQASAHGCPLVKISAGQIVIVEQVQFNKGSADIAEESHHV
                     LEEVERVISAHPELTKISVEGHTDARGPRDLNQLLSARRAAAVVTWLVKRGVAAKRLS
                     SRGFGPDRPIADNEQEEGRQKNRRVEFRIVELDGKPTQTDKPAKPTPEKAPPQKPAQP
                     GETAP"
     gene            complement(3883..>4593)
                     /locus_tag="VFQ61_11340"
     CDS             complement(3883..>4593)
                     /locus_tag="VFQ61_11340"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9955092.1"
                     /translation="TGGALGCSTDEPQAPARARGALLFDDPQLSRSSSNRYACSTCHD
                     TEASPSGSPGTTASKLRKPGAPLAGVTQRASYWNGQENDLLRAMDACLFYFMGSSEKL
                     DPTEPGARALYAYLESLEPGNAAPVPFSLVRNVEDVPRGDASAGGTLFELSCAGCHGT
                     LHDGQGRLTERAPILPEQTLAEHQGYSARLLRLVFIEKTRHGAFFGYGGTMPPFSKEV
                     LSDAELGSILEYLELRGE"
BASE COUNT          813 a         1523 c         1437 g          820 t
ORIGIN      
        1 ctctgccgcc ttttcggcgg cagagctgtt ttgtgcggga ccgctcgggt gttgcttctc
       61 gaaccgcctc gacgagcaaa gccgcactcg accgacacgg caagcgcgtc gtcgtcaaga
      121 ctctgcctca tccgctgaaa cactggagtc cagactaggg tctggagcgg ccattggtcg
      181 ccttctcacc gcgtggtcgt cttctccgcg acgcaccgca aatcaggggc tagcaaatcg
      241 agagcccctg atttcagagg agcgcagtac cgattgggta aagacgtacg gaaccagcgg
      301 cgtcgcgggg atcgctacgc gcgcggggcg tcgctgcgcg ggctcgtggc gggcgtcttg
      361 ctgggatttc ccaactcgct ggaggctttg gaagcgtcac cgagctcgtc cccgatctct
      421 ttcttgaaag agcggatgcc ttgaccaagg cctttgccaa cgttcgcgag gttcttgggg
      481 ccgaacagca gcagaaccac gaccagaacg agtaaccaat gtacggggct gagtgatccc
      541 attcgacgcc tccttctcgc atcgaaactg atgcggacgg caggttcgaa cctacttcaa
      601 actcataggt gcggcaacct gcggagccca cgaacccagg cattcaagcc gtttgtcgtg
      661 cgttgccccg ccgcgcttct cgggcgcagg ggctagtgca ggccaagcac atcgagtatc
      721 aagccgaatg gaccacattt ccgaatcgag gaaatttaca cggcaatgtg cgtcgaaccg
      781 cgccggcgcc gccgcgcgac ccgcagcgcg agtgcgccca aaagacccaa gaggctgctg
      841 tggtgagcag cgactctcag cccatgcaac tggcacccgc cgccgctgat ctgactcgcg
      901 cggttgctcg gggttctcgg cttggtctca ccggatcctg aggcgccggc agcgcccgct
      961 gccccgccca aggaatctga gccgccggag tgagactccc ccgctgggcc aatcgcgggc
     1021 tgccccgctg cccctccggg cccgctcccg gcctcttcct cgccggtcgt tcccgcgacc
     1081 gtgaccgcgc cggccgcgcc ggcctccgcg ttgcgtccac ctgcagctcc ggaatcttgg
     1141 ccacccgcgc ccgcgccgcc cgtgccaatg caactctcga ctccggagtt gcattggccg
     1201 tgcccatcac acgtgtcttg atcgctgcag gcattgccgt catcgcagcg cgtgccagcg
     1261 tccaatggat aatctacgca gccggtcttg ctgttgtccg tgagttcgca agcgcgcagg
     1321 aaacagccgt gaactccgac ttcgcgacac gcggtcacga gctcacaata cggggacccg
     1381 ctgcgcaacg tgcacaggcc ttcgcaagcg ttgcccaacc cgagcggacg acatgccgcg
     1441 ccgaggcgcg cattctttgc cacgcattgg ccattttcac acgacttctc acaactcgaa
     1501 ccgctcgcgt ctccgcagat tcggccattc atatcctcgg cgctcaaccc tgctgaggcg
     1561 atcggaagac actgtgcgga gcggtccgcg ccgatccaac acagcccctc acactcggtc
     1621 gcgtcaccgc cgccgtccgg atcacaaggc agacctacca aatccatgct gcagttggtg
     1681 gtgatggctt gggctttggt gcggggggcg cgcgagcgag atgtgtcggg ttgcgattcc
     1741 actcccgaac acccgaccgt gccgatcgcc gcgaatgggg cccaactact agcggtgatc
     1801 gagagcgggc caaccaagcg caacactgcc ccaaggccgc gtgagatcga gtttcgggca
     1861 atcggtttca tggtgcggtc tcgcccggct gggctggctt ttgggggggc gccttctctg
     1921 gcgttggctt ggctggttta tctgtttggg tcggcttgcc atcgagctcg acaatgcgga
     1981 actccacgcg acgattcttc tgccgccctt cttcttgttc attgtcggca atcggtcggt
     2041 ctgggccaaa acctcgcgaa gataggcgct tcgcggccac gccgcgcttg acgagccagg
     2101 ttacgactgc ggcggcccgg cgggcgctca acagttgatt caagtcccgc gggccccgcg
     2161 cgtccgtatg gccctcgacc gagatcttcg tgagctccgg gtgcgcgcta atcactcttt
     2221 cgacctcctc cagtacgtga tggctctcct ccgcgatgtc ggcgctgccc ttgttgaact
     2281 gcacctgctc cacgatcacg atctggccgg cgctgatctt gacgagcggg caaccatggg
     2341 cgctcgcttg gcccggctcc gtagggcaat cgtcttccgg gtcgaggacg ccatcgtgat
     2401 cttgatcgac gatgggtggc tttcggggac agccgttttc tcggggtgag ttggctgcaa
     2461 ctccgggttc cgcggggcag gcgtcgaggg cgtcgggcac ggtgtccaaa tcggtatcac
     2521 ggggcggaca gccattctcg gcgagcaact tgctggcgat gccaggctgt aaggggcacg
     2581 catcgtatgc gtctgcgatc ccatcgcgat cggtatcagg aggtgtcgcg ggggccttgt
     2641 cactgagccc gccaggcacc cattctacgc tcgccacgac gcggaaagca ggtgtcccca
     2701 aggcttgggt cagcccgcga cccgcgccga caccgatccg cacgtctgat tccaaatcgt
     2761 agtgcgcgcc gagcagaacc tcgagcgggg tgctgttcgg ggcaaagaag ccatccccgc
     2821 catcgctcag caccgtggat gcaaaaaact cgggcccgag cagcaagcga tccgatagcg
     2881 ctcgcagccc gagcgaaccc gcgaaaacca agtccgcgcc gaacgggaca cccgcgaagt
     2941 tgtcggtttg tgcgtgccag cggggcccga gccgaagccc atagctgacc ttcgagacat
     3001 ccccggctac atggaggttc gtagagattc gaaccccacc gtcgcccgtg aacgcggagc
     3061 ggctgcccgt aggtaagcca aaatcgaggc tcacccccgc gcgcaatagg ttcgtcggca
     3121 gacgaagcag ccggtaaagc accccaattc gcacgtcccc gagctgggct ccaccgctca
     3181 atgcgtactg cgtgtccgcg atgcgcccgc catctccgct gtcgacgaga gccaccggca
     3241 aattgagcgc gagccgaagg ttgggcaaaa ccgcgacgct tgctcccagg tgggcgaaca
     3301 gctggtgccc aacatagtca gcggtgcggt tgccctcggg atctcgaaac accagcggtt
     3361 ggcgcgcgta gtcgaacacg gctcctgctg cgaatccgct ggttcgcgcc gcgcgtcgac
     3421 tcaacccgaa gtcgagggac tcgagcgtga accagcggct tccgcgctcg gatggctcga
     3481 atcggtctaa ggcaaagccg cgcgtcgtcg tctcggcgaa cgcagcccgc gccaagagca
     3541 ttgggctcag ggccaatacc aaacttgaaa agaagcgacg catgtgagac cgagcttcgc
     3601 tcccggacgt tcgggttaac gagccagacg gcctgagagc aaacgtgtcc tctatcaagc
     3661 gggacacggg aatccaatgg tcctgacgtc ggggccgctg ggtgtccacg ttcgtcaggg
     3721 aaattgtcgg ctgattctgc tcctcctaga agcagaaccg gctgaactcg taacacctcg
     3781 gcgagtctag tgagtggatt gcgccgagtg gccgagggaa accggttgcc tcgcgcccga
     3841 gcacgcctgg agtgccggtc gagctctcgg gccccgcgct ttctactcac cccgaagctc
     3901 gagatactcg agaatcgagc caagctccgc gtcactcagg acttctttgg agaacggcgg
     3961 catggtccca ccgtagccga agaatgcgcc gtgccgcgtc ttctcgatga acacgagccg
     4021 tagcaaacgt gcggagtaac cctgatgctc ggcgagtgtc tgctccggca aaataggcgc
     4081 tcgctcggtc agtcgcccct ggccgtcgtg aagcgtgccg tgacagccgg cacagctgag
     4141 ctcgaacagc gtcccgcctg cgctcgcatc cccccgcggc acgtcttcca cgttgcggac
     4201 gaggctgaag ggaacgggtg cggcgttgcc cggctccaag ctctcgaggt aggcgtagag
     4261 cgcccgcgcc cccggctcgg tagggtcgag cttctcgctc gaacccatga agtagaacaa
     4321 gcaggcatcc atggcgcgca acaaatcgtt ctcttgcccg ttccagtagc tcgcgcgctg
     4381 ggttacgccc gccagcgggg cgcccggctt gcgaagcttc gacgccgtgg tgcctgggga
     4441 gccggatggc gaagcttccg tgtcgtgaca cgtgctgcac gcgtagcgat tgctactcga
     4501 gcgggaaagc tggggatcat cgaacagcag cgctccccgg gcgcgagcag gggcttgggg
     4561 ctcgtccgtg ctgcaaccca gcgcgcctcc ggt
//
LOCUS       DASTKU010000318         3127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1846822, whole genome shotgun sequence.
ACCESSION   DASTKU010000318 DASTKU010000000
VERSION     DASTKU010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846822"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFQ61_11345"
     CDS             complement(<1..421)
                     /locus_tag="VFQ61_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955093.1"
                     /translation="MRLVISSKGSCRSGQPVSMAREAGGAQLQKPIQLPKAQRTRVLS
                     STMILVGGLCVSALLGACNKDGPKPAPETRALEPAGQGREGHADVQAREAAPAAAAPA
                     AAGPTTPAGTAAPSTISETNFDLSLAAKDGCSVGKPAQ"
     gene            complement(469..1800)
                     /locus_tag="VFQ61_11350"
     CDS             complement(469..1800)
                     /locus_tag="VFQ61_11350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9955094.1"
                     /translation="MKACPQCGRLYPDEGGFCPVDGSALGRATQVPVAVDASDSRIGQ
                     LICNRYQVRRVVADGGMGRVYEALDLQTKQNLAVKVLHQDVASDEVAVERFKREFEVS
                     KQLPHDYIVEVYDFQPTSDSTYAIAMEYLYGEELRATLKREGVISPARLVRMLSQIAL
                     GLDEAHSRNFVHRDLKPDNLFLCQTSDGDIVKILDFGSVKDKGETAKKLTVLGTTIGS
                     PFYMAPEQAQGLDTLDQRADVWALSAIVYECVTGQVPFYGTNGPSILLEILTKEPRPA
                     SEAGKARKYPVPPTLDRVLQKAFKKSPALRTATVGLLADEVGQAYGLSGDHRGWATLP
                     EGQMVQQIDATLPGLMTAPAPAPAKKTSTDDFFGESQALGDSVPPFAAKPAAPSGNAV
                     LASSGTASGAAVRTSDFEAAGIPKRGVPKSLWVSGAVAVGILLVLVVVLLR"
     gene            complement(1853..2599)
                     /locus_tag="VFQ61_11355"
     CDS             complement(1853..2599)
                     /locus_tag="VFQ61_11355"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HET9955095.1"
                     /translation="MTIQKQIEALETLAALDAELGVLNEALNQEREGLTSKQSQLSSL
                     DERYERGRKGVEEMEKSRNDLVGELRQMNFQVERSREKLPRCRTEREANAVQRELEEL
                     RKLVRDREQEIEKIEALLTQARDESTRLSSQRQNLSGELGSQQGEVMSRLATSEQSVS
                     EKNAVRAGLVKQIDPQLYRRYELIRTRRGSAIAHTSNGTCSACHMQLQPMLFQKLMRA
                     DAFGQCPSCNRILYYRPAVPGEVENSSESP"
     gene            complement(3025..3098)
                     /locus_tag="VFQ61_11360"
     tRNA            complement(3025..3098)
                     /locus_tag="VFQ61_11360"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3062..3064),aa:Ile,seq:cat)
BASE COUNT          521 a         1033 c          941 g          632 t
ORIGIN      
        1 cctgagcggg cttgcccaca ctgcatccgt ccttggccgc caacgacaag tcgaaattgg
       61 tttccgatat ggtgctcgga gctgcggtgc cggcgggtgt cgtggggccc gcggctgcgg
      121 gcgcagcagc tgctggtgcg gcttcccgag cctgtacgtc cgcatgtccc tcacgtcctt
      181 gccctgccgg ttcgagggca cgggtttccg gtgcgggttt cggcccgtcc ttgttgcacg
      241 caccaagtag cgcgctgacg cacagcccgc caaccaggat catcgtgctc gagagtacgc
      301 gagtgcgctg cgctttcggc agctgtatcg gcttctgcaa ctgggccccg cccgcctccc
      361 gagccatgga gaccggttgc ccactgcggc acgaaccttt gctcgaaatc acgaggcgca
      421 ttccgatcgc ttaccacacg cgcggcgatc aggaaaacct cggaaaaatc agcgcaatag
      481 gaccactacc aagaccagca agatccccac ggccaccgcg ccactcaccc acaagctctt
      541 cggaacaccg cgctttggaa tgccagcggc ctcgaagtca ctggttcgca ccgcggcccc
      601 tgaagcagtt cccgaagacg cgagcaccgc gttcccggac ggggcagcgg gtttcgctgc
      661 gaacggcgga acggagtccc ccaaggcctg actctctccg aagaagtcgt ccgtggatgt
      721 cttcttcgct ggcgccgggg cgggagccgt catcagtccg ggcagcgtgg catcgatctg
      781 ctgcaccatt tggccttcgg gcagcgttgc ccagccgcga tgatccccgc tgagaccata
      841 ggcctgtccc acttcgtctg ctagcagccc cacggtggcg gtgcgaagag cgggcgactt
      901 cttgaaggcc ttctgtaaga ctcggtccaa ggtggggggt accggatact tgcgagcctt
      961 gccggcttcg ctcgccgggc gaggttcctt ggtcaggatt tcgagcaaga tactgggccc
     1021 gttggtgccg tagaacggaa cctggcccgt gacacattcg taaacgatcg cggacagcgc
     1081 ccacacgtct gcgcgctgat ccagtgtgtc gagcccctgc gcttgctccg gcgccatata
     1141 aaacggcgaa ccgatggtcg tgcccagcac cgtcaacttc ttcgcggtct cgcccttgtc
     1201 tttgaccgag ccaaaatcga ggatcttgac gatatccccg tcactggtct ggcatagaaa
     1261 caggttgtct ggcttcagat cgcgatgcac gaagttgcga gagtgggctt cgtccagccc
     1321 gagcgcgatt tggctgagca tacgtacgag gcgcgctggc gagatcaccc cttcgcgctt
     1381 cagcgtggcc cgcaactcct cgccatacag gtactccatc gcgatcgcgt acgtggagtc
     1441 cgaggtgggc tggaaatcgt acacctcgac gatgtagtcg tggggcagct gcttactgac
     1501 ttcgaactct cgtttgaaac gctcgacggc cacctcgtca ctggcgacgt cctgatgcag
     1561 gaccttgacc gcgaggttct gcttggtctg aagatcgagc gcctcgtaca cccgccccat
     1621 gccgccatcc gcaaccaccc gccgaacttg atagcggttg cagatcagtt gcccgatccg
     1681 gctgtcactc gcgtccacgg ctactgggac ctgcgtggct cgtccgagtg cagagccgtc
     1741 gacagggcag aagccaccct cgtcgggata aaggcgaccg cattgggggc aggctttcac
     1801 gcttcacccc tcgggaagat tgaaccgacc aaagggcgaa gcatgcctgg cctcacggac
     1861 tctccgagga gttttcgact tcgccgggga ctgcggggcg atagtagagg atccggttgc
     1921 agctcgggca ctgaccgaat gcgtccgcgc gcatgagttt ctggaacaac atcggctgca
     1981 gctgcatatg acaagcgctg cacgttccgt tgctggtgtg cgcaatcgcg ctgcctcgcc
     2041 gtgtgcggat caattcgtag cgacggtaca gctgcggatc aatctgcttg accaggcccg
     2101 cgcgtaccgc gttcttctcg gagaccgatt gctcagacgt ggcgagccgg ctcatgacct
     2161 cgccttgttg agaaccgagc tcgcccgata ggttctgccg ctgcgacgag agccgcgtcg
     2221 actcatcgcg tgcctgcgtg agcagagcct cgattttctc gatttcctgc tcacgatctc
     2281 ggaccagctt gcgcaactct tccagctccc gttgcaccgc gttggcctcc cgctccgtgc
     2341 gacagcgcgg cagcttttcg cgcgagcgtt cgacctggaa gttcatctga cggagctccc
     2401 caaccaaatc gttgcgggac ttctccatct cctcgacccc tttgcggccc cgctcgtaac
     2461 gctcgtccaa actggagagc tggctctgtt tgctggtaag accttcgcgt tcctgattca
     2521 gagcctcgtt cagaacgccg agttcggcgt cgagtgccgc aagcgtttcg agcgcctcga
     2581 tttgcttctg gatagtcact ttctgaatcg gctccttcgg aattaagcaa tgcgggcggc
     2641 cgggggcgcc gccccgaacg accaaaacct tagactacta cgactctgcg tccggtgaga
     2701 tgcgcgcctt gccgtcggcg tttcaacgcg cggctcggac gcggctttct ctccgagttc
     2761 attcttgaat tccgtgccca ccgttggcgc gaccaccggc gccgtcgccg tgagccccat
     2821 caaggcggtc gtgcccagcg ccgctgtagt cctcagcgcc gctgtagtcc tcagcgccgc
     2881 tgtagtcctc agcgccgctg tagtcctcag cgccgctgta gtcctcagcg ccgggctggt
     2941 tctcggtgcc gccgttgaga gcggtgctgc accccgcgct ctaaccaccc gcggggctac
     3001 tttggtccgc ggggctgtac gctgtgggcc cacctgggtt cgaaccagga acaacccggt
     3061 tatgagccgg gggctctgac cgattgagct atgggcccgg tctctagcca tttgcgccgc
     3121 cgtattc
//
LOCUS       DASTKU010000319         3301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_508750, whole genome shotgun sequence.
ACCESSION   DASTKU010000319 DASTKU010000000
VERSION     DASTKU010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508750"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFQ61_11365"
     CDS             <1..333
                     /locus_tag="VFQ61_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955096.1"
                     /translation="PPPSSRSQSPTPPPAIESHASTHSVAPTNGASVRPVMQSFRPSQ
                     PAPEIRNATPPAEHVGQRAAARVSVLTNSDQPGLFTVRVLADGEGVQAPAVEAFLVFS
                     EPNVRISR"
     gene            373..1644
                     /locus_tag="VFQ61_11370"
     CDS             373..1644
                     /locus_tag="VFQ61_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955097.1"
                     /translation="MLSKCSKTSFGIRSSVWLFFSAFGGSLIFGCGSDDLDSNASGGS
                     SSGGAASGGASAGKGGTAGTGGTSTGGATGGSGNTGGSGNTGGSAGSGGSSTGGGSSI
                     PDKDCRLASDCGVANAVCDPADGKCKAYQCDATHDCPADQICIAQTENARGGVCHDFC
                     VPFEDTCPNNGECVSLDIDDTEGFCLPRGSVGRGESCEQASISSSCTAGLICASLNEQ
                     SECTQACSFFGAGPACPSDQRCLIFGLCSAPEGDPAGLNEGCDESALPGDFCGDDKTK
                     LAGVCLPFGENDAPTCLQLCSVAQAKCSGPSFQCQQFTTDNLALGVCVDVGECGDGGK
                     EYDDCDACIKAETAGEGCCADLTAECADNQECEGFGTCVTDCGANATEACFDSCVTDY
                     PNGVELFLAWLECSSGSPSGFVGACGVVCAQ"
     gene            complement(1839..3089)
                     /locus_tag="VFQ61_11375"
     CDS             complement(1839..3089)
                     /locus_tag="VFQ61_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ricin and poly(3-hydroxybutyrate) depolymerase
                     fusion"
                     /protein_id="HET9955098.1"
                     /translation="MRTSLHSRSFALMLVSAGLACVGACGTENGVPGGGGTPSVPGAG
                     GQSTSATGGAVASTGGATASGSGGMSAGMSPGGSAAGGSSSSSSAGNPGSGGVIAGGA
                     SSGGKSSAGSSSGGSGGKSSMPNSGGSSSGGKAAGGSSSGGSSAAGAAGSGGAAPGTR
                     SAGCGKTTTLMNGRASIDVSGTAREYILALPTGYDPSRAYRLIFGWHPLGGSAQQVAS
                     GGYYGLKTLAGDQAIFVAPEGLPFSGSNLGWGNDNGRDLAFLQAMLDRFRGELCIDEG
                     RIFSTGFSFGGMMSFAVGCSSMSMMRAIAPMAGNSMVSGCQSGDRPVALMGFHGVDDT
                     VVLISGGRTARDVFVKRNGCSSETMPVEPSWCGGLKDNYKPCSCVSYQGCKEGYPVIW
                     CEYKAGHMAAPSSGATLWEFFSQF"
BASE COUNT          587 a         1073 c         1028 g          613 t
ORIGIN      
        1 ccgccgccga gctcgcgctc tcagtcgccg actccacctc ccgcaatcga gagccacgcg
       61 agtacgcatt cggtggctcc aaccaatggg gccagcgtgc gacccgtcat gcagagcttt
      121 cggccctccc agccggcgcc cgaaattcgc aacgcaaccc ccccggcgga gcacgtaggg
      181 cagcgtgccg cggcgcgcgt gagtgtgctc acgaacagcg atcagccagg cctgttcacc
      241 gttcgcgtct tggccgacgg tgagggagtt caggctcccg cggtggaggc cttcttggtt
      301 ttttccgaac ccaacgttcg catctctcgt taaccatcta accctcgtca ccactcaccc
      361 cggggtctcc tcatgctgtc gaagtgctcc aaaacgtctt tcggaattcg cagttctgtg
      421 tggctcttct tttcggcgtt cggtggcagc ttgatctttg gctgtgggtc ggacgacttg
      481 gattccaacg cctcgggcgg aagctcgagt ggtggcgcag ctagcggagg agcgagcgcg
      541 gggaagggtg ggacggcggg caccggtgga acgagcactg gcggcgccac gggtggttct
      601 ggaaacactg gcggctccgg aaacaccggc ggtagtgcgg ggagcggagg ctcctcgacc
      661 ggtggtggca gcagcattcc cgacaaggat tgccggctcg cttcggattg cggcgttgcg
      721 aacgcggtgt gtgatcctgc ggatggcaaa tgcaaagcct accagtgcga tgccacgcac
      781 gactgcccgg cagatcagat ctgcatcgcg caaacagaaa acgcgcgagg tggcgtttgt
      841 cacgacttct gcgtcccgtt cgaagatacg tgtcccaaca acggcgagtg tgtctcgctc
      901 gatatcgacg atacggaagg gttctgtttg ccgcgcggct ccgtagggcg aggcgagagc
      961 tgcgaacagg cgagtatctc gagcagctgc acggcggggc taatttgtgc gagcttgaac
     1021 gagcagagcg aatgcacgca agcctgctcg ttctttggtg cgggacctgc ttgcccgagc
     1081 gatcaacgct gcctgatctt cggtctgtgc agcgctccag agggcgatcc tgcggggctg
     1141 aacgagggct gtgatgagag cgcgttaccc ggagacttct gtggcgacga caagaccaag
     1201 ctggctggcg tgtgcttgcc attcggagag aacgatgctc ctacttgcct gcaattgtgc
     1261 tcggtggcgc aggcgaagtg cagcgggcct tcttttcagt gccaacaatt cacgaccgac
     1321 aaccttgcgc tcggtgtctg cgtcgacgtc ggcgaatgtg gagacggcgg gaaagagtac
     1381 gacgactgcg acgcatgcat caaagcagag accgcaggcg aaggttgctg cgcggatttg
     1441 accgcggaat gcgccgataa ccaggaatgt gagggcttcg gcacttgcgt gaccgactgt
     1501 ggagcgaacg ctaccgaagc ttgcttcgac tcgtgcgtga ccgattaccc gaacggagtg
     1561 gagctgttcc tagcgtggct ggagtgcagt tcgggttcac cgagcggctt cgtgggcgct
     1621 tgcggtgtgg tgtgcgccca atgaattcga gcgcgcgatc cctaagcgtc tgatacagcc
     1681 cccgaccgcg taaccacgca aaaggggccg ctcgctcgag cggcccctgc gcggtgcccc
     1741 tactgtgctt ttactgtgct cctcgtgacc tctctcgggc ggtgggggga accgacggaa
     1801 cccccgcacc tgctgcagcc tcgccgattt tgcgcctctc agaattgaga gaagaactcc
     1861 cagagcgtcg cgccggagct cggagccgcc atgtggcctg ccttgtattc acaccagatc
     1921 acgggatacc cctccttgca accctggtag ctcacgcagg agcacggctt gtagttgtct
     1981 ttgagccccc cgcaccagct cggttcgacc ggcatggttt cgctcgaaca accgttgcgt
     2041 ttgacgaaca catcgcgagc cgtccgacct cccgagatga gcacgacggt gtcgtcaact
     2101 ccgtggaaac ccatgagcgc caccggccga tcgccgctct gacagccgct caccatcgaa
     2161 ttaccggcca tcggtgcgat ggcccgcatc atactcatgg acgagcagcc cacggcaaag
     2221 gacatcatgc cgccgaagct gaatcccgtg gagaatatgc ggccctcgtc tatgcaaagc
     2281 tcgccccgga agcggtccag catggcttgt aggaaggcca agtcgcgacc attgtcgttg
     2341 ccccagccca ggttgctgcc gctgaacggc aggccctctg gcgcgacgaa aattgcctgg
     2401 tctcccgcca gtgtctttag gccgtagtaa cccccgctcg ccacctgctg cgctgagccg
     2461 cccagcggat gccaaccgaa gatcaatcga tatgcgcgcg acggatcgta gcccgtcggc
     2521 aacgccagaa tgtactcgcg tgcggtgccg ctcacgtcga tactcgctct accgttcatc
     2581 aacgtagtcg tcttgccgca gcccgcgctt cgtgttccgg gggctgcgcc gccgctgcct
     2641 gctgcgcccg cggcgctcga cccgccagag ctagaaccgc cggcggcttt gccgccactg
     2701 ctcgagccgc ccgagttggg catggagctc ttacctccgg agccgcccga actggagccg
     2761 gccgagctct tgcctcctga gctggcgccg cccgcgatga caccccccga acccggattg
     2821 cccgcgctgg agctgctcga gcttcctcct gccgcgctgc ctcccgggga cattcccgca
     2881 ctcattccgc cagagccgct agctgtcgcc cctccagtgg atgcgactgc gccaccggta
     2941 gcagaggtgc tctggcctcc cgcgccgggg acgcttggcg tgccgccgcc ccccgggacg
     3001 ccgttttctg ttccgcaggc tccgacacaa gcgagacccg cactgacgag catgagagcg
     3061 aacgagcggg aatgaagcga cgtgcgcata gaaacctcct acgagaactc cgactactag
     3121 ttagtagtct caggcctcaa tatcagggca gccgcggctg ctcacaggcg ccaatttcga
     3181 ccctcgatca atcgcggaaa cttttgccca tactcgccac ggcggcgcgt ttggcggtcg
     3241 gtcataggca gacctttcca gggtcggagc acctgcatac aggccacacc gtaatcgctc
     3301 c
//
LOCUS       DASTKU010000320         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1401806, whole genome shotgun sequence.
ACCESSION   DASTKU010000320 DASTKU010000000
VERSION     DASTKU010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401806"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1569)
                     /locus_tag="VFQ61_11380"
     CDS             complement(139..1569)
                     /locus_tag="VFQ61_11380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HET9955099.1"
                     /translation="MHPETERKIANILAHEFDEPGVTIPFTKRLARENGWTESYSARV
                     INEYKRFLALAVAGDPMVSPSDAVDQAWHLHLQYSARYRKFCQNVLQQNLDHRPSEGG
                     EAELSRYKLAYGQTLERYEELFGEPPPADIWPAPSERFRQEHAVRVTIDDHWVIAKAQ
                     FVHRTLLVLVAAIALIASLSTSISGATYLYGYVTLWFACLIAAFVARRVADGPEPEPY
                     PTLDHYELLQLASGPLGAVDGAVARLIAEQAIEFEPSLQRLKLRGPLPATATDLERRV
                     YEKIPEESSGISVRELRRTARELTPFTTRRLKELGLITERHFDNAFWLALVAPAFALI
                     RIVSRLGTDKPIGFLLLLFAVGLFVAFRTSRRSGRTRWGDQLLARARAEHPNQPAVAP
                     TSSTQIPLSLALFGATAVTVPIAASWLDWVSSKRYTASTSSGACASGCGGDGSGCGGG
                     DGGGGCGGGDGGGCGGGCGGCGGGGD"
     gene            complement(1611..>2649)
                     /locus_tag="VFQ61_11385"
     CDS             complement(1611..>2649)
                     /locus_tag="VFQ61_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955100.1"
                     /translation="ILLMAAAQRAELRRSVLDVNAYAASGQAAAVLYQLREYADTVER
                     AASDPAVRALTRGPLKRPFPGTNDGADQDPCRSQVSLEVPTALEPYSSRFATLTVLDE
                     QGCARARISEEAAPAEYARHSYSWRDYFASAARDAERPGRITYVRPAYRSSVSQQIKF
                     AVSRPLFEGERWIGVVYGSMVAASTLDLPRMKRSDTSERMTVLIGPFEGERSGPQDPY
                     TGQEFTLLAHPRLERGRKVSLTPSTARELVQALGRPGKDQRQFELSTSLPYQRADHVD
                     PLLGGRWLAAFAPVGATGYVVLVQTRDDLAVRPSNGLWRTGFVLAFGSAALLGVWGAF
                     FVWRWRREHPR"
BASE COUNT          470 a          833 c          883 g          463 t
ORIGIN      
        1 gtgctcgcgc tgctgcgcgc ggccgaggtc ctcaacgcca tgctcgctcc gcgggagctc
       61 cgctgcgcgt ggcggaggag cctttcgtcc tcggttctct gcgcggagca agcccttcaa
      121 cgtgcgcaag cgttcggttc aatccccgcc gccgccacat ccaccacacc ctccgccgca
      181 tccgcccccg tcgccacctc cgcagccacc accgccatct ccgcctccgc agccgctacc
      241 gtcacctccg cagccactgg cgcacgcgcc gctggacgtg ctcgcggtgt agcgcttgct
      301 gctgacccaa tcgagccagg atgcggcgat cggcacagtg acggcagtgg cgccgaatag
      361 tgcgagcgat agcgggatct gggtactgga ggtgggggcg acggcgggtt ggttggggtg
      421 ttcggcgcgg gcacgcgcga gcaattggtc accccatcgg gtgcggccgc tgcggcgaga
      481 cgtgcgaaag gcgacgaaca atccaactgc gaacaggagt aacaagaagc caatgggctt
      541 gtccgtgccc aagcggctca cgattcggat tagggcgaat gcaggtgcaa cgagggcgag
      601 ccagaaggcg ttgtcaaaat gccgttcggt aatgaggccc agttccttta gccgtcgtgt
      661 ggtgaacggc gtgagctcac gcgcagtccg gcgcagctct cttaccgaga tccccgatga
      721 ctcctcgggg atcttctcgt acacccgacg ttctagatcg gtggccgtcg ccgggagtgg
      781 tccgcgaagc ttcaagcgct ggagtgaggg ctcgaattcg atagcttgct ctgcaatcag
      841 gcgcgcgacg gcaccgtcaa ctgcgccgag cggcccggat gcgagctgca gtaactcgta
      901 gtggtcgagc gtcggatacg gctcgggctc gggaccgtcg gccacgcgcc gcgcaacgaa
      961 ggcggcgatc aaacaggcaa accacagggt gacgtagccg tatagatagg ttgctcccga
     1021 gatgctcgtg gacagactgg ctattagcgc aatcgcagcc acgagcacca gtagagtccg
     1081 gtgcacgaat tgagccttgg caatgaccca atgatcatcg atcgtgaccc gcacggcatg
     1141 ctcctgtcga aatcgctcgc tcggagctgg ccagatgtcc gcggggggag gttcgccgaa
     1201 gagctcttcg tagcgctcga gggtctgccc gtacgcgagt ttgtagcggg aaagctcggc
     1261 ttcgccgccc tcgctcgggc ggtgatcgag attctgttgc agtacgttct ggcagaactt
     1321 gcggtatcgc gcggagtact gcaggtgcaa gtgccaggct tggtcgaccg cgtcggaggg
     1381 ggagaccatt gggtcgccgg ccacggctaa cgccaagaag cgcttgtact cgttgatgac
     1441 gcgcgcacta tatgattccg tccaaccatt ctcgcgcgcg agacgcttgg tgaagggaat
     1501 agtgaccccg ggctcgtcga actcatgggc caggatgttc gcaatctttc gttcggtttc
     1561 aggatgcata taaccctcca ccgaaatctt tacgtgcgac gtggagttcc ttaccgcgga
     1621 tgctcgcgcc gccagcgcca cacgaagaag gccccccaaa cgccgagcaa tgccgcggag
     1681 ccgaaggcca gcacgaaccc ggtgcgccaa agtccattgc ttggacgtac ggcgaggtca
     1741 tcgcgggttt ggacgagcac gacatagccg gtggccccga cgggggcgaa ggccgcaagc
     1801 caacgtccgc cgagcagcgg atccacgtgg tctgcgcgtt gatagggcag ggacgtgctc
     1861 agttcgaact ggcgctggtc tttgcccggg cgtccgaggg cctgcactag ctcgcgcgcg
     1921 gtgcttgggg tcaagctgac tttgcgccct cgctcgagcc gaggatgcgc gagcagggtg
     1981 aattcttggc ccgtgtacgg atcttgcggt ccagagcgct cgccttcgaa agggccgatc
     2041 aacacggtca tgcgctcgct ggtgtcacta cgcttcatgc gcggcagatc cagcgtcgac
     2101 gccgcgacca tgctgccgta cacgacgcct atccaacgct cgccctcgaa cagcggtcgc
     2161 gaaaccgcga acttgatttg ctgggacacc gaggagcgat acgcagggcg cacgtacgtg
     2221 atgcgcccag ggcgctccgc atcacgcgcc gcgctggcga agtagtcacg ccagctgtag
     2281 ctgtgccgcg cgtattcagc gggcgccgct tcttcggaga tgcgcgccct cgcgcagccc
     2341 tgctcatcga gcactgtaag ggtggcaaaa cggctggagt acggctcgag cgcggtggga
     2401 acctcaagtg acacttgcga gcgacatgga tcctggtcgg cgccatcgtt cgtgcccggg
     2461 aatggcctct tcaaggggcc gcgcgtgagc gcgcgcacgg ccggatcgga agcggctcgt
     2521 tccacggtgt cggcgtactc gcggagttgg tagagcacgg cggcggcttg tccactcgcc
     2581 gcgtaggcgt tcacgtcgag cactgagcgg cgtagttccg cgcgctgcgc cgcagccatg
     2641 agcagaatc
//
LOCUS       DASTKU010000321        15798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1624556, whole genome shotgun sequence.
ACCESSION   DASTKU010000321 DASTKU010000000
VERSION     DASTKU010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 15798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15798
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624556"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..987)
                     /locus_tag="VFQ61_11390"
     CDS             complement(73..987)
                     /locus_tag="VFQ61_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9955101.1"
                     /translation="MTTTLLGVDDSKTMRKVLEITFAGENYKTVLAANADDAMSKLQV
                     ERPSVMLVDAALEGTNGYELCQRAKAAAPGVFVILLSSKQQPYDKARGSQVGADDYID
                     KPFDTQQLIDKVSSVLRRPQAPAPAPVVAPPPAAQPPIAARPADVAAAPRQRTQTIAY
                     GSHVSPPQGASPVAPVIPAGGMAAVPPRSSPATASSAFSPAARPAPAPITLDSVPPPA
                     ATPAPAPAAPLSSPPKPAAAAPATNGADFAPKAEFSQKLSGLGLTPEQVTAVLALSRE
                     VVEKVVWEVVPTLAETMIREEIQRLTRD"
     gene            1460..2506
                     /gene="hrcA"
                     /locus_tag="VFQ61_11395"
     CDS             1460..2506
                     /gene="hrcA"
                     /locus_tag="VFQ61_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET9955102.1"
                     /translation="MNPLQKRARQVLCALVTEFIGTGEPVGSRTLTRKYGFTLSPATI
                     RTVLADLEESGYLVQPHASAGRVPTPQAFRIFIDALMQERTLPENEAHRINEHFTELR
                     PGADVLRETGKLLSDLSGAPAVLIRLRADQRALSKIHFIRTRPQELLAVLVATDGTVD
                     NRFIQVDRPGDDAELDRLHRLLDEVVSGKTLRDVRDHFAREAENSHDEIARLNRLAHD
                     LLGAAAQGLERRADVVIEGQARLLDQPEFGSVNSLRELMAAFEDRERLVKLLDQAIFA
                     DRVNVFLGEETRQMVGCAVSLVAAAYRDEDGAPTGAVGVIGPERMDYPFVVPLVGATA
                     EAMTVMLQKKREPS"
     gene            2572..3945
                     /locus_tag="VFQ61_11400"
     CDS             2572..3945
                     /locus_tag="VFQ61_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9955103.1"
                     /translation="MVDAQAAGSSARSSSTLPAPAPERPIDRALSHVARGEPAEALRY
                     AIPLLEQEPRNALRCLVLGRALSIGGRSAVAERVLRRAIDLAVEQSNLPLAVAAAAFL
                     DLPADEKRSVFEPLAQAFARGSERLSDRRVVPPSFPAPAEAFEPLPESVDAEALLGRA
                     ERVVPEDSSSAEFGSRRSLLPQPLFSTLIADDLCSFMSIFDARVVEQGTCIIEEGELG
                     DQAFVVARGEFDVQKRGARDGEEPVHLARVGAGALIGEMALLSRAPRVATVVAARPGI
                     VLIAQKSALDQLVSRAPEIGRRFADHCKRRMVENLVRTSEMFRAASPGERPALVERFV
                     LRTFEPGEKLATQGVPSEGLFLIASGDVAIQHVDGAERTLVAKLGPGDVVGEIALVFR
                     RSSVADAVAQQPTVTLCLPRERFPELVRFHQKVFCDLYELAAKRDQEIEFIAQEEATE
                     GEDFVIV"
     gene            4130..4762
                     /locus_tag="VFQ61_11405"
     CDS             4130..4762
                     /locus_tag="VFQ61_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HET9955104.1"
                     /translation="MTRCSPARAFGSLLTATALLFSASAFAQTPTRIGVVDTQRAIME
                     TEDGLRAQATLKKLFDSRQRELDKKQEELQKERDDIEKQKDVLSKAALAKRVDKWQRE
                     MVQLQTVFVEYNKELQKKQNELTQPIFQKAMGLIRRLATQEAVDVIVDKQAVPYVRSD
                     LDLTDKVIILYNQGVAPEQDPKAKPAKPSTAVPTLKAPDATPPAVPAPKP"
     gene            complement(4989..7535)
                     /locus_tag="VFQ61_11410"
     CDS             complement(4989..7535)
                     /locus_tag="VFQ61_11410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9955105.1"
                     /translation="MPIDREKVLQAAQKYVEKKKYDGAIAEYQKIVQEDPNDARTLLK
                     IGDLQARLQAYPEAIATYDRVGQYYANQGFSLKAIAVYKQIREIIKKHAPELVDRYAH
                     IGPKLAEIYTQLGLTSDALSAWDEVAARLQRAGRDREAVDVFRKMVELDRQNPLPHLR
                     LAEACCRVQALDDAVDSFWTAADLLLNLGRQDDALKVIERILHFRPDPRYARVAAELY
                     LARPTREDGLQALAKLQLCFQADPKDLETLGLLGQAFTSIGQADKAVEVYKEMARVAR
                     ELGRVDVYDGLIEHLRQVAPEDDQVRAFESFLPPSPPIESEGPQSIDDSEVELLDDAL
                     LKSDPRGLSNPGSSPDVMLIDDSDEEVPEELESPSSFDARNHAKKATVDAESFRKLRL
                     YPRAIETLRSALRIDPRSTEIRVKLREILLESGDSAGALAESVNLAALYLDQGDGGAA
                     EPLLRQVLENEPGHVGARALLQQLPQNQRRSEGALPRAPVATRFENYEDYDPSAPLPS
                     YDLEEVSADQALYHRAPEPERHSATQAEAPLPSFPLGSEEPFEGAIEDLDEIQDVEEL
                     PPQGAIEAPAADSDALEQALEEAEFFVARGLFDDAKAILEEQRTRTPGHRLVLERLRE
                     IEELKLKSGRSQTIERSQIGGTKDSFLDLTASLNALDALELPEDQAPRHRTTADAEQV
                     FAQFKAGIRAQVSENDSATHYDLGVAYKEMGLIHDAVHELEIAARDPDRECMCHAMVG
                     LIHLEQNQLDRAAESYIRALAAERKTVEQEMNLYYDLGNVYEMKQKTEDALYYFQKIA
                     RRDPGYRDIAERIAVLSAPKKPGREPSGTRAVNDDEFERVFDDLFESKIK"
     gene            complement(7767..8699)
                     /locus_tag="VFQ61_11415"
     CDS             complement(7767..8699)
                     /locus_tag="VFQ61_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET9955106.1"
                     /translation="MRLRSHRVAERFLEAEAYRLAASLSFYSLSSMFPLMIVALAAAD
                     TLLGDSAELRNTLISAVNGTNSPALRALLEDTLREVLDHAPEQHLRIIVGLLGAVFGA
                     SGIFLELVAAMDKLFRVAPPKGSFLHQVWYALRGRGSAILLVFGTSLVLLCGTVVLGT
                     IDVIAARLPAWAESLPGLVTDVSSVGVTWIFLTLCYRVVPNTRVPIRAAMWGAGVAAI
                     IMRAVRWPVTWVVAHVTTYSAYGVVGTLLVLVTWFYVVSSVLLLGAAWTAVHGAVEEL
                     GIPPETRASDPETEPQKDTRGASSGNSQRPTVST"
     gene            10140..10418
                     /locus_tag="VFQ61_11420"
     CDS             10140..10418
                     /locus_tag="VFQ61_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955107.1"
                     /translation="MRRSLAAARNEESEKPNELLIPERLGIHVSQGPAARGGTHPEFL
                     GFLGERLNLSESDAVEVLGRLLSEYRAKSKYEISMLSPVRKCGERFEP"
     gene            complement(10434..11312)
                     /locus_tag="VFQ61_11425"
     CDS             complement(10434..11312)
                     /locus_tag="VFQ61_11425"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9955108.1"
                     /translation="MTSANPDRPEERSGERTGEVIGQRYRLGNLIGRGGQGAVYRARD
                     LKAGDEVAIKILREFNPRDVNLRERMLREAHALTVLRGTAAVQVLHQAWANDGSYCLV
                     MELLEGQNFEDHLRQREARGEKLNLAELSRYLEPVVATLERAHHAGILHRDLKPSNLF
                     ILHTGQVRLMDFGFAKFTRMASLTQAGLVAGSPNYIAPEIWQGRTDFDQRIDVYSLAA
                     VSFRALAGRTPFTSERLDELLNQVTRAPRPKLSPERPDLPRAVDDWFEHGLAIDPSHR
                     FERVRAMWNALDILAH"
     gene            complement(11342..13381)
                     /locus_tag="VFQ61_11430"
     CDS             complement(11342..13381)
                     /locus_tag="VFQ61_11430"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HET9955109.1"
                     /translation="MKLRPGGRMRAEWGRHDFESFVACLRFVQARHFGGIASLRSVWT
                     RLLLRPVARKLLAEHGLQPPADALLFQLDPEEKDLEHRFTTEEAWSAAREAASSGLVR
                     ICEQRSSDLAHRLGAAFEFLGNLELDTRGEGLSIVGAGRNKRTGTFYTPAAVAREVAR
                     RALDEFARVRGPELAPLWVCDPACGSGAFLLEFTRELWLRRQATATGDSSYEGVLRSS
                     VCGIDLDPLAIAVCELCLWSAVGRSETPARGLVRLHAGDALTGRGFAERPYLAEPSAL
                     DLRAAFPDRIDDGFDLIIGNPPWVAFAGRAAQPLEPELRRFFARAYAGFRGYPTLQAA
                     FVERALELAPRGIVALLVPSPIADLDGYRPIRRRLFTTHRPAEPLLEFGQDAFSEVTQ
                     PCFALVATPRAKSTGEPPPPSSNAPNDDRAWLLSERPRLAQSPAPLRIPDVLERLRDG
                     PRLPAELFGEMGLQSTRLVTEHLFLRNSAPSGDFTYPLLEGKDVSEYRVGPVRLYLRP
                     DPDLLARARCRLRAEQDYQRVALVVRQTARAPIAALHNGLPFRNSLLAGFRTDPWPAD
                     LLVGLLNSSLYRALHVSRQRDARQSTFPQVKLAHLRSLPRPPFDETLWARLRELVQRA
                     SMSGADPDLKRAIDAAVFSLFGLNSSDAERVVEFLASRSPDLASCIQARPRHDSS"
     gene            complement(13378..14481)
                     /gene="pdxA"
                     /locus_tag="VFQ61_11435"
     CDS             complement(13378..14481)
                     /gene="pdxA"
                     /locus_tag="VFQ61_11435"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET9955110.1"
                     /translation="MPSPSRTRGTPIAHRAHSPEPSLLVVSVGCPAGIGPEVSVRAAA
                     NLPKIPCVLVGDAEVIARAAELSGVSSKKLVPFDGSAPSAGRIAVLQAGPRLSRRDLN
                     PSRPSPRAGEAQLAYIERAYELVAKRPERALVTGPVSKAAIAHSGLARARGFLGHTEW
                     LRDLDGKRASVMCFHSEKLVSALVTTHLPISAVPQAIRSADVATASVELARLLLRLGT
                     HRPEIAICSLNPHAGESELLGKEERRAILPGIEAAQRKLGKTALLVGPVGAETAFRKA
                     HAGGYDGVIAMYHDQATIPMKLVAFGDAVNVTLGLSIVRTSVDHGTAYDIAWKGQANA
                     SGMEAAMKLAARLVETQPPATRQPKRLKKSGRA"
     gene            complement(14589..>15798)
                     /locus_tag="VFQ61_11440"
     CDS             complement(14589..>15798)
                     /locus_tag="VFQ61_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9955111.1"
                     /translation="IWTEPWEATLVNRELETRSKPHQLQKLESLALLAGGIAHDFNNL
                     LVGILGNVGLLLLDTPEGSPLLPGLEDIQSAALRAADLTKQMLTYSGRGRFVAQNTDL
                     NRWVEEVVKLFSSALAQNCSLDLRLGPAPLFVEADPSQLRQLILNLLTNAADALADRA
                     GRITVATGHLHADHKYLETCFADDTVLTEGEYAFIEVTDTGPGISPGMVDRIFDPFFT
                     TKFQGRGLGLPAVLGILRGHGGAIHVKSEPGKGAAFRALLPVVAPRAEKPSIPSSERG
                     LSFVGTGCILVADDEETVRTISERVLNQAGFDVLLASDGTEALALYRANSSKVSLVLL
                     DMTMPGMTGKEVFEAIRNVRSDARVLLTSGYTEEDVTSNFAARSLSGFLPKPWLPGEL
                     LAAIKRALEN"
BASE COUNT         2939 a         4893 c         5040 g         2926 t
ORIGIN      
        1 cgccgcagga ggagcgacga tcgggcgccc gagtcgcccg aatgattcgc tccgttcctc
       61 gttcagtttt ggctagtcgc gcgttagacg ctgaatttct tcccggatca tggtttcagc
      121 caaggtcgga acgacctccc aaacgacctt ctccacgacc tctcgggaga gcgcgagcac
      181 tgccgtcact tgctccggag tgagacccag gccggagagc ttctgagaga actcggcctt
      241 cggagcgaag tcagcgccgt tcgtggccgg cgctgccgcg gccggcttcg gcggcgagga
      301 caaaggcgcg gctggggcag gagcgggggt agccgcgggt ggcggcaccg aatccagggt
      361 tatcggtgcc ggtgcaggcc ttgcggcagg cgaaaacgct gacgaggccg tagcgggact
      421 cgagcgcggt ggaacggctg ccattccgcc cgcagggatc accggggcga ccggcgaagc
      481 gccctggggc gggctcacat ggctgccgta cgcgatggtc tgggtgcgtt gtcgcggcgc
      541 cgccgccacg tctgcgggcc gcgctgcaat cggtggttgt gccgcaggcg gcggagcgac
      601 cacgggcgcc ggcgcgggcg cttggggacg cctcagcacg ctgctcacct tgtcgatgag
      661 ttgctgcgtg tcgaaaggtt tgtcgatgta atcgtcagcg cctacttgcg agccgcgagc
      721 cttgtcatac ggctgttgtt tactcgaaag taggatcacg aatacgccgg gcgcagccgc
      781 tttggcgcgc tggcaaagct cataaccgtt cgtgccttcc agcgcggcat cgaccagcat
      841 gacgctgggt cgctccacct gcagcttgga cattgcatcg tccgcgttcg cggccaaaac
      901 ggtcttgtag ttttcgccag cgaaggtgat ttccagcacc ttgcgcatag ttttgctgtc
      961 gtcgacgccc agcagcgtgg tggtcacaga gcctccaatg agattttacg aacggctaag
     1021 aatcatcggg ggccgggcaa accctgtcaa ggttttcacg gggcgggtgg ttgtgcgctg
     1081 gatgaagcac atgcgactcc aacgcgactt atcgaagtca gccattttcg ccggggcagc
     1141 tctgtgggcc ctcgaggcgg ggcttactgg cggcctttgc ggactgggac tgtaggtata
     1201 gttcgtagtc aggggttcac gggcagcggt tcacggactc ggctagagcg gatctgcagc
     1261 ggcgttgctc gcgttcactc gaccgtgtct gcacgaccat gtctgcagtg cgcgggcggc
     1321 tcttccgcga ggatttaggc caaatctacg ggggttacag gcgggcggca gtctctcggc
     1381 gactcggcgg cctgcgtcgt ggaccagcca gagcggacgg gatttgactt gaggccgttc
     1441 gtgcggccaa aattctcgcg tgaatcctct acagaagcgt gcccgtcagg tgctctgtgc
     1501 tctcgtcact gagttcatcg gaacgggcga gccggtcggg agtcgcaccc tcactcggaa
     1561 gtacgggttc acgttgtccc ccgccacgat ccgcacggtg ctcgctgatc tggaggaatc
     1621 tggctatttg gtgcaaccgc atgcgtcggc tggccgggtg ccgacgcccc aagcatttcg
     1681 catcttcata gatgccctga tgcaagagcg cacgctccca gagaatgagg cccatcgaat
     1741 caacgagcac tttaccgagc tgcggcccgg tgccgacgtg ctgcgtgaga cgggcaaatt
     1801 gctgtcggat ttgagcggag cccccgccgt cctaattcgc ctgcgcgccg atcaacgagc
     1861 tctcagcaag attcacttca ttcggacccg cccgcaggag ttgctggcgg ttctcgtcgc
     1921 gaccgacggc accgtcgata atcgcttcat tcaagtggac cgtcccggcg acgacgccga
     1981 gctcgatcgc ctgcatcgtt tgctcgacga ggtcgtttcc gggaaaacgc tacgcgacgt
     2041 gcgcgatcac ttcgcgcgtg aagcagagaa cagccacgac gagatcgctc ggctcaaccg
     2101 cctcgcgcac gacctgctcg gcgccgcggc ccagggactc gagcgtcgcg cagacgtggt
     2161 gattgaaggg caggcacgcc tactcgatca gcccgagttc ggaagtgtga acagcctgcg
     2221 cgagctgatg gcggccttcg aagaccgtga gcgtttggta aagctcctcg accaagccat
     2281 cttcgcagat cgcgtgaacg tcttcttggg tgaggagacg cggcagatgg tgggttgtgc
     2341 cgttagcttg gtggccgccg cctaccgcga cgaagacggc gctcctaccg gggcggtcgg
     2401 ggtaatcggt ccggagcgta tggactaccc cttcgtagtt cccctcgtag gtgccaccgc
     2461 ggaggccatg actgtaatgc tccaaaagaa gcgcgagcct agctaaccca cccgcagcaa
     2521 cagaaggcgt gatcagcgga gcgggagtga tagccttggt cgctattcat catggttgac
     2581 gctcaagctg ccggttcgtc cgcacgctcc tcctcgaccc tgccggcgcc cgctcccgag
     2641 cggcctatcg atcgtgcgct ctcacacgtg gctcgtggcg aaccggccga ggcgttgcgt
     2701 tacgccattc ccttgttgga gcaggagccg cgcaatgctt tgcgttgcct ggtattgggg
     2761 cgcgccttga gtattggcgg gcgcagtgca gtcgccgagc gcgtgttgcg tcgagccatc
     2821 gatttggcgg tcgagcagag caacctgcca ttggccgtcg cggcggcggc attcttggat
     2881 ttacccgcag acgagaagcg gtcggtgttc gagcccctgg cccaggcttt cgcccgcggc
     2941 tcggagcgct tgagtgatcg tcgcgtcgtt ccacccagct ttcctgcgcc cgcagaggct
     3001 ttcgaaccct tgcccgaatc cgtcgatgca gaggcgttgc ttggtcgagc ggagcgggtc
     3061 gtgccagagg actcgagcag cgcagagttc ggctcgcgac gatcgctgct cccgcagccg
     3121 ctgttctcga cgctgatcgc ggacgatctc tgctccttca tgtcgatctt cgacgcgcgc
     3181 gtggtggagc aaggcacctg catcatcgag gagggagagc tgggggacca agcgttcgtc
     3241 gtggctcggg gcgagttcga cgtgcaaaag cgtggcgcac gggacggcga agaaccggtc
     3301 catttggcgc gcgtcggggc tggtgctctg atcggagaga tggctctgct ttcacgggcg
     3361 cctcgcgtag ccacggtcgt ggctgctcgc cccggcatcg tgctgatcgc gcaaaagagc
     3421 gcgctagatc agctggtgag ccgtgccccc gagatcggcc gccgcttcgc cgatcactgc
     3481 aagcgccgga tggtcgagaa cctggtgcgc accagcgaga tgtttcgggc cgcgagcccg
     3541 ggagagcgtc ctgctttggt ggagcgcttc gttctgcgca cgttcgagcc gggggagaaa
     3601 cttgcgacgc aaggcgtgcc gagcgaggga ttgttcctga tcgcttcggg tgatgttgcc
     3661 attcaacacg tcgacggggc cgaacggact ttggtcgcga agctgggccc cggggacgta
     3721 gttggtgaga tcgccctggt cttcaggcgc agttccgtcg cggacgccgt cgcgcaacag
     3781 ccgacggtca ccttatgtct accgcgagag cgtttcccgg agctcgtgcg cttccatcag
     3841 aaggtgtttt gcgacctgta cgagctcgcg gccaagcgtg atcaagagat cgaattcatc
     3901 gcgcaagaag aggcgaccga gggcgaggac ttcgtgatcg tgtgatgccg ctagattccg
     3961 agtgcgggcg tttcggtggt cggtggaggc ggccggtcgc cgacggagcc gcgccggacc
     4021 ggcaaagcgt cacgtcgtga gggtgcgcct gcgcattttg agcgcgagtc gagccacgcc
     4081 gcgcggactg accttgaccg cctcccgggc cgaggctacg cccccgcgca tgacacggtg
     4141 ttcgcccgcc cgagccttcg gctccttatt gaccgctacc gcccttctct tcagtgcttc
     4201 ggcttttgcg cagaccccga cccgaatcgg cgtggtcgat acccaacgcg ccatcatgga
     4261 gacggaagat ggcctgcgcg cccaggctac gttgaagaag ctcttcgaca gccgtcagcg
     4321 cgagctcgat aagaagcagg aagagctaca gaaagagcgc gacgacatcg aaaagcagaa
     4381 ggatgtgctc agcaaggccg cgctcgccaa gcgggtcgac aagtggcaac gcgagatggt
     4441 gcagctgcag accgtgttcg tcgaatacaa caaagagctt caaaagaagc agaacgagct
     4501 cacccagccg attttccaga aggccatggg gctcatccgt cgcctcgcga cccaagaggc
     4561 cgtggacgtg atcgtggaca agcaggccgt tccatacgtg cgcagcgatc tcgatctgac
     4621 ggacaaggtc atcattctct acaatcaggg cgttgcgccg gaacaagatc ccaaggcgaa
     4681 gccggccaag ccgagcacgg cagtgccgac gctgaaagcg ccggacgcga ccccgccggc
     4741 ggtcccggcc ccgaaaccgt gagccgaggc gcgccccact gaagccttgg tgtgagaaaa
     4801 cctggatgag agaccgcccg gcggggcact ctcatccagt cgtcgtggtg cgcggggcgg
     4861 ccgtgggctg atcggcaagc gttccttgga tcagcaagga tcattaggaa gcatgacgcg
     4921 accagcgagg tcagcggcgg ctccaagctc gccggacgtt ggcggtatcg aagctccgag
     4981 cgcctcgcct acttgatttt gctctcgaac agatcgtcga acacgcgctc gaactcgtcg
     5041 tcgttgactg cccgtgtgcc gctcggttct cgcccgggct tcttcggcgc ggagagaacg
     5101 gcaatgcgtt ccgcaatgtc tcgatagccg ggatcgcgac gcgcgatctt ctggaagtag
     5161 tacagcgcgt cctcggtctt ctgcttcatc tcgtaaacgt tgccgaggtc gtaatacagg
     5221 ttcatctcct gctcgactgt cttacgctcc gcggcgagcg cccggatata cgactccgcg
     5281 gcgcgatcga gctggttttg ctcgagatga atgagcccca ccatggcgtg gcacatacac
     5341 tctcgatcgg gatcgcgcgc cgcaatctcc agctcgtgca cggcgtcgtg gatgagcccc
     5401 atctccttgt acgctacccc taggtcatag tgggtcgcgc tatcgttctc ggagacctga
     5461 gcgcgaatac ccgctttgaa ctgcgcgaaa acctgttcgg cgtccgcggt cgtgcgatgc
     5521 cgcggcgcct gatcttccgg gagctctagc gcgtcgagcg cgttcaacga ggccgtgaga
     5581 tcgaggaagg agtctttggt cccgccgatt tgcgagcgct cgatggtctg gctgcgcccg
     5641 ctcttgagct tcagctcttc gatctctcgc aggcgttcga gcaccaagcg atgccctggg
     5701 gtgcgcgttc gctgctcctc gaggatggcc ttggcgtcat cgaacaatcc tcgcgcgacg
     5761 aagaactctg cctcttcgag cgcttgctcg agtgcgtcgg agtctgccgc gggagcttcg
     5821 attgcgcctt gaggcgggag ctcttctacg tcctgaatct cgtcgagatc ctcgatcgcg
     5881 ccctcgaacg gctcctcgct gcccaagggg aagctcggta atggcgcctc ggcttgtgtc
     5941 gccgaatgcc gctccggttc cggcgcgcgg tgatagagcg cctgatcggc cgatacttct
     6001 tctagatcgt aggaagggag gggcgcgctc gggtcgtagt cttcgtagtt ttcgaagcgg
     6061 gttgcgaccg gtgcgcgcgg taaagccccc tcgctgcggc gttgattctg tgggagctgc
     6121 tgcaagagag ctcgcgcacc cacgtgtccg ggctcatttt cgagcacctg gcgcagcaac
     6181 ggttcggcgg cacccccatc accttggtcc agatacaagg ccgcgagatt gacggactct
     6241 gcgagtgcgc ccgccgagtc cccagattcg agcagaatct cacgaagctt gacgcgaatc
     6301 tccgtcgagc gcggatcgat gcgcagcgcc gaacgcaagg tctcgatggc tcgtgggtag
     6361 agccgcagct tgcggaagga ctcggcgtcg accgttgcct tcttggcgtg atttcgcgca
     6421 tcgaatgaag acggtgactc gagctcctcg ggcacctcct cgtccgagtc gtcaatcaac
     6481 atgacgtccg gagagctgcc gggattcgag agcccgcgtg ggtccgactt caacagcgcg
     6541 tcatcgagca gttcgacttc ggaatcgtcg atcgactgcg gcccttcgct ctcgatgggc
     6601 gggcttggag gcaagaagct ctcgaacgcg cgaacctggt catcctcagg ggcaacctgc
     6661 cggaggtgct cgatcaaacc gtcgtagacg tccactcgac cgagctcgcg cgccacgcgc
     6721 gccatctcct tgtagacctc gaccgctttg tcggcttgac cgatcgacgt gaacgcttgc
     6781 cccagcaagc cgagcgtctc gagatccttg ggatcggctt ggaagcaaag ttgcagcttg
     6841 gccaaggcct gcaacccatc ctcacgggtt ggcctcgcca ggtacagctc cgccgcgacc
     6901 cgcgcgtagc gcggatctgg tcgaaaatgc aggatgcgct cgatcacttt cagcgcgtcg
     6961 tcttgccgac cgaggttcaa cagcagatcc gccgcggtcc agaacgagtc caccgcatcg
     7021 tccaaggcct gcactcggca gcaggcttcc gctagccgca agtggggcag cggattttgg
     7081 cgatcgagtt ccaccatttt gcggaacacg tccaccgctt cgcgatctcg ccccgcgcgt
     7141 tgcagtcgcg cggccacttc gtcccaagcc gagagcgcat cgctcgtcaa cccgagctgg
     7201 gtgtagatct cagctagctt cgggccgatg tgcgcgtaac ggtcgaccag ctccggcgcg
     7261 tgctttttta tgatttcgcg gatctgcttg tagaccgcga tggccttcag cgagaaaccc
     7321 tggttcgcgt agtactgccc cacgcgatcg taggtcgcaa ttgcctcggg gtaagcctgc
     7381 agacgcgcct gaagatcacc aatcttcaaa agagtccgag catcgttcgg atcctcttga
     7441 acgatcttct ggtactccgc gatcgcaccg tcgtacttct tcttctcgac gtacttctgt
     7501 gcggcttgga gaactttctc gcggtctatc ggcactggct gacaaggaag cggcgtgggg
     7561 gttggcccga cgccggacca ggccgctcga caggagttta ggggccattc cagagggggt
     7621 caagtaaagc tcgggaaact ttggataatt tccgcgagaa tagcggcgtt ggtcaggagc
     7681 tcttgaacgc ctggcgttgc cctgtgtcag ggctcagcag ggctgccccg cgggggcgtg
     7741 gtcagcgcag gcgcgggggg agtggttcac gtgctcaccg ttggtcgctg actgtttccg
     7801 gagctcgcgc cgcgggtatc tttctgcggt tcggtctccg gatcgctcgc gcgggtctcc
     7861 ggcggaattc caagttcttc gacggccccg tgcaccgcgg tccaagcggc tccgagcagc
     7921 aaaacgctgc tcacaacgta aaaccaggtg accaagacga gcagggtacc aaccacgcca
     7981 taggccgaat acgtcgtcac gtgcgccacg acccaagtga cgggccagcg caccgcgcgc
     8041 atgatgatgg cagcgacgcc ggcgccccac atcgccgccc gaatggggac tctcgtgttg
     8101 ggtaccacgc ggtaacagag agttagaaat atccaggtca cccccacact ggagacatcg
     8161 gtgactagcc cgggtaggga ttccgcccag gcgggtaggc gagcggcaat gacatcgatg
     8221 gtgcccagaa ccacagttcc gcagagcagc accaagctcg tcccgaacac cagcaggatc
     8281 gcagacccac gtcctcggag cgcgtaccac acctggtgca agaaggagcc tttgggcgga
     8341 gcgacccgga atagcttgtc catcgccgcg acgagttcca ggaagatgcc gctcgcgcca
     8401 aagacagcac caaggagccc cacaatgatg cgcaggtgtt gctcgggggc gtgatcgagc
     8461 acctctcgca gcgtgtcttc caatagagca cgcaacgctg gcgaattggt cccgttcacg
     8521 gccgagatca gggtgttacg cagttccgca gaatcaccga ggagcgtgtc cgccgcggct
     8581 agcgcgacga tcatcaacgg gaacatggaa gaaagcgagt agaacgagag cgaagccgct
     8641 aagcgatacg cttccgcctc caagaagcgc tccgcgactc ggtgtgaccg gaggcgcaac
     8701 cggctgagcc aggaatctcg cttcaaccag gagctgtacc gatctgaaag cgcgttgtac
     8761 ttcacgtccg tcgatgatcg ccacccggtg gctgcggatg caactccgcg cacgaagctg
     8821 cttcaaaaca gctactccgc gctcacgtcc cgttatgcgt cgctcgcctt tcgtgcaggt
     8881 tgcgagaatg agggtccctc gagctatccc ccggattcga ggcaccccat tcgcagctgt
     8941 cgagtttcgt cgttagtacg cgaatcgggc gagccaaccc ttcagaccac ccccgttgcg
     9001 acaaggccac cctctctcga accggaaacc cggcgtgcaa acaatcgaca agcacacatg
     9061 atctcgaaca gctgcagctt tcctcggcaa ccgagctgct cttaaagcgc atctctctag
     9121 tccgttacgg agggcacccc gattgtgctg gaaccagcac atgaatctcg acggttatgg
     9181 aagcgacagc atcgggaaac ctgaagtctt ttctattggc cgtagcggcg tgtaacgggt
     9241 ccaaaatcac tcagcgcttc tgcgccgaga acgcgaaatt ttagcgcgct gctgctcatt
     9301 cgaacgcgtt actcgcgcga cccaaccgct gctgaatgct cgaccaaaca gacgcataaa
     9361 cagagcgggc aggcgcgcat aaacagcgcg ggcaggcgcg cataaacagc gcgggcaggc
     9421 gcgcataaac agcgcggcca ggcgcacaaa caaacagcgc ccaaaacaga accgaccgga
     9481 gtacgaaaac agccagctaa tcgcgcgtcg aagaacgccg gcttgaaaga tcttgcttct
     9541 tgcggttgcg ccgagatgca cggcttcgaa aatgcacggc ttcgaaatgc acggcattcg
     9601 aaatgcacgg cttcgagatg cacggcgcaa gcaggatcca aacgagctac gcggccaagt
     9661 ctggcgatac tcgacctaga tcttttgttg tttgaaaggt gctttcgacg gcgattgttg
     9721 ttattctccg ccttcactcc ggatgcgact ggggcatcgg ccgcggtggc ttgcacacgg
     9781 acatgtgctc gcaatgggac cccgggtagc aggtcgaggt tcaacgaggc cctttgggtg
     9841 tttaggtggt cgacggcgcg acagcggcca acaccggaaa ccttacgatg agttccgttt
     9901 cacggaatca gtattctcta aagtgcacac atcatcttgc atgtacccag cgcagcgcga
     9961 gtacccagcg cagcgcgaat acccagcgca gcgcgaatac acgacgcagt gggaactcga
    10021 atttcagttg cgcacacgaa caaccagcgg cttacgccgt aacccaagcg cttacgcacg
    10081 ccgagtatca acgctcgaaa ccgttttgag aaatttcgtc caggagagga ggtagtcgcg
    10141 tgagaagatc ccttgccgca gctaggaacg aagaatccga gaagcctaac gagctactca
    10201 tccctgagag attaggcatt cacgtttcgc aggggcccgc cgcacggggc ggtacacacc
    10261 cagagtttct aggtttcctc ggagagcggc tcaacctgag cgagagcgat gcagtcgagg
    10321 tattgggtcg gttgctttcg gagtatcgcg ccaagtccaa gtacgagatc agtatgctca
    10381 gccccgtacg taaatgcggt gaacgcttcg aaccgtagcg ccttgcgctt cgctcaatga
    10441 gcgagaatat cgagcgcgtt ccacatggca cgcacccgct cgaaacggtg gctcggatcg
    10501 atggcgaggc cgtgttcgaa ccagtcgtcg acggcgcgcg gcagatctgg gcgctctggg
    10561 ctcagcttcg ggcgcggcgc gcgagtcacc tgattcagaa gctcatccaa tcgttcgctc
    10621 gtgaagggtg tacggcccgc tagggcgcgg aaagaaacag cggcgagcga gtagacgtcg
    10681 atgcgctggt cgaagtccgt acgtccttgc cagatctcgg gcgcgatgta gttcggtgaa
    10741 cctgctacta ggcctgcttg ggtgaggctc gccatgcgag tgaatttggc gaacccgaag
    10801 tccatcagtc gcacctgccc cgtgtgcagg ataaacagat tgctgggctt caggtcgcgg
    10861 tgaaggatgc cggcatgatg ggcccgttcg agagtggcca ccaccggctc caggtagcgg
    10921 ctcagctctg ctagattgag cttctcgccg cgcgcctcac gttgtcgcag atggtcctcg
    10981 aagttttgcc cttcgaggag ctccatcacc aggcaatacg aaccgtcgtt ggcccaggct
    11041 tggtgcagaa cctgaaccgc cgccgtgccg cgcagcaccg tgagcgcgtg ggcttcgcgc
    11101 agcatccgct cccgtaagtt gacgtcgcga gggttgaatt cgcgcaagat cttgatggcg
    11161 acttcatcgc ccgctttgag atcccgtgcg cggtacacgg cgccctgacc acccctcccg
    11221 atcaagttcc ccaggcgata gcgctgacca atgacttctc cggtgcgttc accagagcgc
    11281 tcttcgggac gatcggggtt cgcgctggtc atggcagtgc cgaacaggcg tctccgttag
    11341 attagctgga atcgtggcga gggcgagcct ggatgcagga cgcgagatcc ggtgaccggc
    11401 tggcgaggaa ctccacgacc cgctctgcat cgctcgagtt cagcccgaac agcgagaaaa
    11461 cggcggcgtc gatggctcgc ttcagatctg gatctgctcc cgacatgctg gcgcgttgca
    11521 ccagttcacg caggcgcgcc cacaaggttt catcgaaggg agggcgaggc aagctgcgca
    11581 ggtgcgcaag tttcacttgc ggaaacgtgc tttgacgggc gtcgcgttga cgcgacacgt
    11641 gcagcgcgcg gtagagcgag ctgttcagta gtccaaccag cagatcggcg ggccaaggat
    11701 cggttcgaaa accagccagc aacgaattgc ggaaaggtaa cccgttgtgg agtgccgcga
    11761 tgggggcccg tgccgtttgc ctcaccacta gcgcgacgcg ctggtaatcc tgctctgcgc
    11821 gcaagcggca gcgagctcga gcgagcaggt ccgggtcggg cctcaaatag aggcgcaccg
    11881 ggcctaccct gtattcggag acgtctttgc cctccaggag gggatacgtg aagtcgccgc
    11941 tcggtgccga gttacggagg aagaggtgtt cagtgacgag ccgggtagac tgtaacccca
    12001 tctctccaaa tagctccgcg ggcagccgcg gaccatcccg gaggcgctcc aagacgtcgg
    12061 ggattcgcag gggcgccggc gactgtgcca agcggggccg ctccgacagc aaccaagctc
    12121 gatcatcgtt cggcgcgttg ctgctagggg gcggcggctc gcccgtgctc ttggcgcgag
    12181 gcgtggccac caatgcgaaa cagggttgtg tgacctcgga aaacgcgtct tgcccgaact
    12241 ccagcaaagg ctccgccgga cgatgcgtcg tgaacaagcg cctccggatt ggtcgatagc
    12301 cgtccagatc tgcgatcggg ctcggcacga gcagggccac gatccctcgc ggtgcgagct
    12361 cgagagctct ctccacgaaa gccgcctgca acgtgggata gccgcgaaag ccagcgtacg
    12421 cgcgcgcgaa gaaacgacgt agctcgggct cgagaggctg ggcggcccgg cccgcaaacg
    12481 cgacccaggg cggattgccg atgatcaaat cgaatccgtc gtcgatgcga tccgggaatg
    12541 cggcacgaag atccagcgcg ctgggctcgg cgaggtatgg gcgttccgcg aatccacgtc
    12601 cggtcaaagc atcaccggcg tgcagtcgca ccaagcctcg tgccggcgtc tctgagcgac
    12661 ccacggccga ccagaggcag agctcacaga ccgcgatggc aagcggatcc aggtcgatcc
    12721 cgcagaccga gctgcggaga acaccctcat agctcgagtc gccggtcgcg gtggcttgtc
    12781 gtcgcagcca cagctcgcgc gtgaactcga gcaagaaagc accggagcca caggcgggat
    12841 cgcagaccca aagcggggcc agctccgggc ctcgcactcg agcaaactcg tccagggcgc
    12901 ggcgcgccac ttcgcgcgcc actgccgcgg gcgtgtagaa ggtgccggtg cgcttgttgc
    12961 gtcccgcacc cacgatcgaa agtccttccc cgcgggtatc cagctcgagg ttgccgagaa
    13021 actcgaacgc ggcgcccagg cggtgggcga gatcagagct gcgttgctcg cagatgcgga
    13081 ccaagccgga actcgccgcc tcacgcgcgg cgctccaggc ttcttcggtc gtgaagcgat
    13141 gctcgagatc cttctcctcg ggatccagct ggaagagtag ggcgtcagcg ggaggttgaa
    13201 ggccgtgctc tgcgagcaac ttccgagcga cgggccgcaa gaggagccgt gtccagacgc
    13261 tgcgcaagct cgcgatgccg ccgaagtgtc gagcctggac gaacctcagg caggcgacga
    13321 acgactcgaa atcgtggcgc ccccactcgg ctcgcatgcg cccccccggc ctgagcttca
    13381 tgcacgccca gatttcttca ggcgcttcgg ttggcgcgtg gcgggcggct gggtctcgac
    13441 gagtcgagcc gccaacttca tcgcggcctc catgccgctc gcgttcgcct gacccttcca
    13501 cgcgatatcg tatgcggtac cgtggtccac gctcgtccgc acgatggata aaccgagcgt
    13561 cacgttgacg gcgtcaccga atgcgacgag cttcatgggg atcgtggctt gatcgtgata
    13621 catggcgatg acgccgtcgt agccgcccgc gtgtgctttc ctaaacgccg tttcggcacc
    13681 gacgggtccc accaacaacg ctgtctttcc gagcttccgc tgcgcggctt cgatccccgg
    13741 gagaatcgca cggcgctcct ctttgccgag cagctcactc tctcccgcat ggggattgag
    13801 cgagcagata gcgatttcag ggcgatgcgt cccgagtcga agcagaagcc gagctagctc
    13861 cacgcttgcc gtggccacgt cggcggatcg aatggcttgc ggcacggccg agatcggaag
    13921 gtgagtggtc acgagggcgg agacgagctt ttccgagtga aagcacatga cgctggcccg
    13981 cttgccatcg aggtcgcgga gccactcggt atgtcccaaa aagccccttg cccgagccag
    14041 acccgagtgc gcgatcgccg ctttgctgac ggggcccgtg accagggcgc gttctgggcg
    14101 tttcgccacg agttcgtagg ctcgttcgat gtacgcaagt tgggcttctc ccgctcgcgg
    14161 cgacggccga gaggggttca agtcgcggcg cgaaagccga gggccagcct ggaggacggc
    14221 gatcctccct gcgctaggag cgctgccgtc gaagggcacc aacttcttac tactcacgcc
    14281 cgaaagttcg gctgctctgg cgatcacctc cgcgtcaccc accaaaacgc agggtatctt
    14341 aggaaggttc gccgctgcac gaaccgagac ctcagggccg atgcccgcag ggcagcccac
    14401 ggatacgacg agcagggatg gctcaggcga atgagctcgg tgcgctatcg gcgttcctcg
    14461 cgttcggctt ggggacggca tcgaagcgct cgttacatcg ctagattgcg gggggcaagt
    14521 gctctagacc tcacccccgc gcgccacacc tggtgcccga gccttagccc cctcctgcgc
    14581 agctcgcgtt agttctccaa ggctcgtttg atcgcggcca gaagttcacc gggcagccag
    14641 ggtttgggca agaagcccga aagcgagcgg gcggcgaaat tggacgtaac gtcctcttcc
    14701 gtgtacccgc tcgtgagcaa cactcgggcg tcgctccgta catttcggat tgcctcgaac
    14761 acctctttgc cagtcatgcc gggcatcgtc atgtccaaaa gaacgagtga aaccttcgac
    14821 gaatttgccc gataaagcgc caatgcctcc gtcccgtccg acgccaggag cacatcgaat
    14881 cccgcctggt tcagcactct ttcgctgatc gtgcggaccg tctcctcgtc atccgccacg
    14941 agaatgcatc cggttcctac gaagctcagt ccgcgttcgg agctcgggat ggagggtttt
    15001 tccgcccgcg gtgcgacgac tggcaggagc gccctgaagg ctgcgccttt ccccggttca
    15061 cttttgacat ggatggcacc accgtgacct cgcaggatcc cgagtacggc aggcaagccg
    15121 agccctctgc cttgaaactt cgtcgtgaag aatgggtcga agatccgatc gaccatgccg
    15181 ggggaaattc ctggtcccgt gtcggttact tcgatgaagg catattcacc ctcggtcaat
    15241 accgtgtcgt ccgcaaagca cgtctccagg tacttatggt ccgcgtgcaa gtgaccggtc
    15301 gcgaccgtga tccgtcctgc ccggtccgcg agggcgtcag ccgcgttggt tagcagattc
    15361 aggatgagct gcctgagctg ggacggatcg gcttccacga agagtggcgc ggggccgagg
    15421 cgcaagtcca agctgcaatt ttgcgccaac gccgaggaga acagcttcac gacttcctcc
    15481 acccagcgat tgaggtccgt gttttgcgct acgaagcggc cacgcccaga gtaggtgagc
    15541 atttgcttcg tgagatccgc ggctcgcaag gctgcggact ggatgtcctc caggcccggc
    15601 agaagcggcg agccttcagg cgtatctagc aagagaaggc ctacgtttcc gaggatccct
    15661 acgagcaagt tgttgaagtc gtgcgctatg cctccggcca agagcgcaag gctctcgagc
    15721 ttttgcagct gatgcggctt cgaccgagtc tcgagttcac gattgacgag agtggcctcc
    15781 cagggctcgg tccagatc
//
LOCUS       DASTKU010000322         3740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1177977, whole genome shotgun sequence.
ACCESSION   DASTKU010000322 DASTKU010000000
VERSION     DASTKU010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177977"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2494)
                     /locus_tag="VFQ61_11445"
     CDS             complement(<1..2494)
                     /locus_tag="VFQ61_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955112.1"
                     /translation="MRLLTRFPLGSGSSRALSGAVLLLPLASLAASSCSQRSVAVQLR
                     PLQASGQVSYICRDEGGHGRPIAECNSEALDRGTRELYALLTQTVSGEVAVINVPWAP
                     TTAETGRWFVDQDPTSPGYNFIRVGSRPGSIVSSPGGEATFVTVGEAGKPGIFALPTS
                     CVGRPEPSAPRRDLTTWPACSLTSSPGEMKLVVQAPQDGAIYESCLGSALEQPRVDDP
                     ARECPADLTTEPGPKGRRKLVVTLPDEGKLVVVDAQWLLDQPQGSFGRCQIEAEHPLE
                     VQLPASPVKPVFPPDLANACSEALPSPPVPPAFAPRPSGLALSEDRLYVGDRSAPVIH
                     VLDMSNVCTPKELQPLLPQSFERPGRVVTTTRLAVSPLTPLGKRFLYAVDELDTPRSS
                     LMAFDVSPGSTERTPIVRPGAEYMPYEAPDRIQFAAPVRDVAFIERDRPVVDPVTGAI
                     TVGTACDPEPKSSSLGVAYRSNSDYTRGARARELRGVFGAALLTNGRVQIVDVEDFDA
                     PCRRPVNARMSPDKFSGCADTREFNYYAVSSSTSSLRIVTDELSCRMVEPHRARSSTL
                     AIASADAFTEAPSLRSWPTFKNAPGAEEPGPLDGPQMLGVDFDTRPSSINATDVNVNP
                     MGDKVHLGRARVYLGNTLYDSQYRAENPLSVNPNTATNHSLVLPFVEPRAYNVNEDLL
                     LTYQGQITGTVPAGFLRQEGERWILSDPAFAFCDTGVHDVELMRELGAERFALAGDAL
                     ESFAVEHADRLVLRGDFPAEEDGYWDSLAQVDGRDIDRNFCIEQFGRFDADKLDERRK
                     FRILDAEQQRLEIEPMPRNDRVSPELRMQYAHA"
     gene            complement(2497..>3740)
                     /locus_tag="VFQ61_11450"
     CDS             complement(2497..>3740)
                     /locus_tag="VFQ61_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955113.1"
                     /translation="PGSGRIFVPVRGDATLHWADVGPDGHVACGQENNGGACDDLHRA
                     GNDPEQENTRDLRLGAEPFGIAASWNGEAIMVTNQTSGAASLFVNPWLNQENDAGPKL
                     EFRLEQLSSRPVGVTAVPPPARILAQPAVRNGYAPAFLVTFRNTPEVVLVRYYSDSEY
                     VGRQEGSPNNVASPSRPFVTRLGTAFASPNSPGTDSRGIVADDASRRELEQRCFAAAG
                     ITDSCLSDYGVNGKEGCIAQAGGAGAALDDCLAQASRQPLDFFVASRSPASLLVGSSW
                     VTNDAIGNNEIPSFYDSIPLTVGPSRVVTGEVIVGGDAQVITDAQEDTLIRERRVFVV
                     SFDSRRVFIYDPVRRRLDAEVTTGRGPYAISVDSKHGHLYIGQFTDSFISVVSLDRRH
                     PRTYAQVLATIGAPSAPRTSK"
BASE COUNT          692 a         1226 c         1183 g          639 t
ORIGIN      
        1 aggcgtgggc gtactgcatc cggagctccg ggctgacccg atcattccgc ggcatgggct
       61 cgatctcgag ccgctgctgt tccgcatcca aaatgcggaa cttgcgacgc tcgtccaact
      121 tgtccgcgtc gaaacgaccg aactgctcga tgcagaagtt gcgatcgata tcccgcccgt
      181 cgacctgcgc gagcgagtcc cagtagccgt cttcctccgc ggggaagtca ccgcgaagta
      241 ccaatcggtc cgcgtgctcg actgcgaacg actcgagcgc gtcacctgcg agcgcgaagc
      301 gctcggctcc tagctcacgc atcaactcga cgtcgtgcac cccggtgtca cagaacgcga
      361 aagcgggatc gctcaggatc caacgctccc cttcctgcct caggaagccc gcgggaaccg
      421 tgcccgtgat ttgaccttgg taagtgagaa gcagatcttc gttgacgttg taggcccgtg
      481 gctcgacgaa cggcagaacc agtgagtggt tggtagccgt gttcgggttc acggacagcg
      541 ggttttcagc gcggtactgg ctgtcgtaaa gcgtgttacc gagatatacg cgtgcacgac
      601 cgaggtgcac cttgtccccc attgggttga cgtttacatc ggtggcgttg atcgaggagg
      661 gcctagtatc gaaatccacg cccagcatct ggggtccgtc gagcggaccc ggctcttccg
      721 cacccggggc attcttgaag gtgggccaac tcctcagcga cggcgcttcc gtgaaggcgt
      781 ccgccgaggc gatcgcaagc gtgctcgaac gggcacgatg cggctccacc atccggcagc
      841 tgagctcgtc ggtgacgatt cgcaacgacg acgtcgacga agataccgcg tagtagttga
      901 actccctggt atccgcacag ccgctaaact tgtcgggcga catccgcgcg ttgactggcc
      961 gacggcacgg cgcatcgaag tcttcgacgt ccacgatctg cacgcgaccg ttggtcaaca
     1021 gtgcggcccc gaaaacgccc cgcagctcgc gcgcgcgcgc cccgcgcgtg tagtcactgt
     1081 tggaacggta ggccacccca agcgagctag atttcggctc cggatcacag gccgtgccca
     1141 ccgtaatcgc ccccgtcacc ggatcgacga ccgggcggtc tcgctcgatg aacgcgacat
     1201 cccgcaccgg agcagcgaat tgaatccgat caggggcctc gtagggcatg tactccgcgc
     1261 cgggccgcac gatgggcgtg cgctccgtcg agccggggct gacgtcgaac gccatcaatg
     1321 acgaacgagg tgtatccagc tcgtccaccg cgtatagaaa gcgcttgccg agcggggtga
     1381 gcgggctcac ggccaagcga gtcgtcgtca cgacccggcc cggtcgctcg aaggattgag
     1441 ggagcagcgg ctgcagctct ttgggcgtgc agacgttact catgtcgagc acgtgaatga
     1501 ccggcgcgga gcggtcccca acgtagagtc tgtcctcact cagcgccaac ccactcgggc
     1561 gcggcgcaaa agcgggcggt accggcggag acggcaaggc ctcgctgcaa gcattggcca
     1621 aatccggcgg gaatactggc ttcacggggc tggccgggag ctgcacctcg agcggatgtt
     1681 ccgcctcgat ctggcaacgg ccgaagctgc cttgcggttg atccaacagc cactgcgcgt
     1741 cgacgacgac cagcttgccc tcatcaggca gggtcaccac cagcttacgc ctgcccttgg
     1801 ggccgggctc ggtcgtgaga tctgctggac actcgcgagc tggatcatcg acgcggggct
     1861 gctccagcgc ggacccgagg caactctcgt agatagctcc atcctgcggc gcctgaacga
     1921 cgagcttcat ctctccgggc gaagaagtca gcgagcatgc gggccaagtc gtcaggtcgc
     1981 ggcgcggcgc gctgggttcc gggcggccaa cgcagctcgt cggcaacgca aaaatgcctg
     2041 gtttacccgc ctcgccgacc gtcacgaagg tcgcctcccc tcccgggctg cttacgatgc
     2101 ttccggggcg ggacccaact cggatgaagt tgtagccggg cgaggtcgga tcctgatcga
     2161 cgaaccagcg cccggtttcg gcggtggtcg gagcccaagg cacgttgatg accgcgacct
     2221 cgcccgacac ggtttgggtc agcagtgcgt acaactcgcg ggtaccgcga tcgagcgctt
     2281 cgctattgca ctctgcgatc gggcgcccgt gtccaccttc atcccgacag atgtagctca
     2341 cctgcccgct tgcctgcagc ggacgcagct gcacggcaac cgagcgttgg ctgcagctag
     2401 acgcggccag cgaggccagg ggcaaaagca gtacggcgcc cgaaagcgcc ctcgaggatc
     2461 ccgaacctaa gggaaagcgc gtcaacaatc tcatgatcac ttggacgtcc tgggggccga
     2521 cggtgcgcca atcgtggcga gcacctgggc gtacgtacgg gggtgacgcc gatccaaact
     2581 cactacgcta atgaacgaat ccgtgaactg accgatgtaa aggtgcccgt gcttcgaatc
     2641 cacgctgatc gcgtacggac cgcgaccggt ggttacttcg gcgtcgagcc gacgccgcac
     2701 cggatcgtag atgaagacac gccgtgaatc gaaagaaacg acgaacaccc gtcgctcacg
     2761 gattagcgtg tcttcctgcg cgtcggtgat tacttgcgca tcaccgccga cgatcacctc
     2821 gcccgtgacc acgcgcgacg gccctacggt gagcggaatg ctgtcgtaga acgaagggat
     2881 ctcgttgttc ccgatggcgt cgttggtcac ccatgaactg cccacgagca gactcgccgg
     2941 tgagcggctc gccacgaaga aatcgagcgg ctgcctggag gcctgcgcca ggcagtcgtc
     3001 cagcgccgcc ccggcgcccc cggcctgcgc aatgcaaccc tctttgccat tcacgccata
     3061 gtcactcagg cagctatcgg tgatcccggc cgcagcaaag cagcgttgct ccagctcacg
     3121 gcgcgaagcg tcgtcggcaa caattccacg cgagtcagtg cccggcgaat tcggactcgc
     3181 aaaagcggta ccgaggcggg tgacgaacgg gcgggacggg ctcgcgacat tgtttggaga
     3241 gccttcctgc ctgcctacgt actccgagtc gctgtagtag cgcaccagca ccacttccgg
     3301 cgtgttgcgg aaggtgacca gaaacgcggg cgcgtatccg ttgcgtaccg cgggctgtgc
     3361 gagaattcga gcaggcgggg gcaccgcggt aacccctact ggtcgcgaag aaagctgctc
     3421 caatcggaac tccagcttcg ggcccgcatc attctcttgg ttgagccaag ggttcacgaa
     3481 cagcgaggcc gcgccgctgg tctggttggt gaccatgatg gcctcaccat tccagctcgc
     3541 cgcgataccg aaaggctcgg ctcccagccg cagatcgcgc gtgttctcct gttcgggatc
     3601 gttgcccgcc cggtgcaagt cgtcgcaggc gcctccgttg ttctcttggc cgcacgcgac
     3661 gtgcccatct ggtccgacgt cggcccaatg cagcgtcgcg tcgccacgaa ccggaacgaa
     3721 gatacggccg ctgcccggca
//
LOCUS       DASTKU010000323        23767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_63658,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000323 DASTKU010000000
VERSION     DASTKU010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 23767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23767
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63658"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VFQ61_11455"
     CDS             complement(<1..999)
                     /locus_tag="VFQ61_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET9955114.1"
                     /translation="MSASSSIANAQQQSPKKNSLFRREALDHHLTRELEIDGQVLRVV
                     PPWSWTLFWMLVAIVASTVVVSFVAEVELTSRGKGVLRAPGGVRTLQTQVGGVVTEVS
                     AKSGQSVLVGEPLVKLESAGVQAALLEADRQLELVRSNLKSFTTKQEEIHRTRTALLR
                     SHAELLKQRVASQENTIARLEAKVGSYNALNDKGMVGAIARDEVVEELDRARRQRIEL
                     DESLSQTSLELATLNSAHQSELWKAEQQIQEAETKRTALAFSLQQTLIQAPQDGYVEA
                     VLVRAGDVITAGAPVGKLISTGAAKHVVAFLPEKDRAFVEIGSRVRLEVDQLPYSEF"
     gene            1089..2471
                     /locus_tag="VFQ61_11460"
     CDS             1089..2471
                     /locus_tag="VFQ61_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HET9955115.1"
                     /translation="MKICIFLGPSLAIEEARRLLPEATFLPPAKMGDICTLASSHDPP
                     DVIGVIDGVFEQVPSVWHKEILFAISRGIQVYGASSMGALRAAELHPFGMRGVGRVFE
                     AYACGEYEDDDEVAVVHAAASAGHKPLSEAMVNIREGLRLARQDGLIDDPTHQSLIDM
                     AKGQFYPERSWRGLFRLVSQSGIAKERLTRLAEFIAERDPNIKRADAMALLERVRADV
                     ATAAPPYEPDYQFSSTVFWKKLQQSASSHGPSIGALGQVANGAAAQNAVLIHAKVAEP
                     RWREVQRDGLLRVLIQDETRRRGLEADEQRISAAAQSFRRERGLVSGEATRAWLTENE
                     LSREEFSSLMEIEALYELVLEQERARVDQIAISELKRAGRLGELRREVNVKISHLAQL
                     GIDGPNVEDTGNSPTELLKWYETQFGALQCPLSEHALRIGFASSADFLRAVAELYLAE
                     RQAMRTAAAE"
     gene            complement(2582..2953)
                     /locus_tag="VFQ61_11465"
     CDS             complement(2582..2953)
                     /locus_tag="VFQ61_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955116.1"
                     /translation="MRIWLFFQEGGWSMWFVLLLGIITLAAAGGFARHPHSAREQVVT
                     FFSRATICAIVSGVALNLATVGSQVSTRPEWAQSPQIHLLVLSGIAESLAPAILGFSF
                     LAVSWVLMAVGQRRFNSTHAS"
     gene            3149..3928
                     /locus_tag="VFQ61_11470"
     CDS             3149..3928
                     /locus_tag="VFQ61_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9955117.1"
                     /translation="MLIFLSRQRAPSVDRSVHEPAPAAPGRPAPPFGEAAEPDVPDTH
                     ATARALEAAAMPEPHAGTTELPGEESAEASREPADSLTIAALARGDRRAFDAVYEQHR
                     APLYTFLLRLSGRAALAEDLLQETWLRLVHHAAALPPGTQLRPWLFTVARNLHTSHRR
                     WALLDGSRLLDLAFQPRGSSPSPLEAMAATEAQRALERALTRLPLADREALLLCVVSG
                     FEPSEAASILNISAEATRQRLARARAKLKVLLRSNEASDEP"
     gene            complement(4388..5827)
                     /locus_tag="VFQ61_11475"
     CDS             complement(4388..5827)
                     /locus_tag="VFQ61_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955118.1"
                     /translation="MKPIGLIAPALLATLSWGCGFESRGDGQLRVELLEPAGFYADGM
                     SRRIVRVSFEHRITAGKPLRATTSLGIINPAADDAGTRRLELQTSHGSPIDFVLYAEQ
                     VAGVGRITVEGPGGLRQDADFVLEPVNDELSILVSAGPYIADGAHETTLTIGLTSGSP
                     AFRDVLVKTSHGILSPDKANEAARARTVRVAPNRPRDLTWILGNSAGNAVVSAELGNV
                     LETEIVQLDQALRDLTIGAPESALADDQTVVPILLDYAAPLGELETVTLTTTLGELNA
                     AGTTPEERRTKRITVRGQETALVNLSVGRQPGPALLTAHSGTTPDRAVSLWLQPALPD
                     RIALELLDGSVLDSSHSQLQIRSTLTRDAGRGLVSAGTTIWLHPCCEIDSGSLEDCTR
                     YLAVPSFVQDVDGDGRESAIAALTPAGTNLVQTAASGQQKNVAVTLFAFVMEEGSPSI
                     ACTDWQNEPPRRVAAWQQIALALARPAQP"
     gene            complement(5824..6081)
                     /locus_tag="VFQ61_11480"
     CDS             complement(5824..6081)
                     /locus_tag="VFQ61_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955119.1"
                     /translation="MPRILEANLKTPGVYEVRVRPDGSAAASGRILASADELGLEVRA
                     VGTWCSKDNEDGCGEANTDGGVWLFAEQTARSPAFPIVRIS"
     gene            complement(6091..8040)
                     /locus_tag="VFQ61_11485"
     CDS             complement(6091..8040)
                     /locus_tag="VFQ61_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955120.1"
                     /translation="MPSLAWRMRGRSSLGVALVSAQVFWSGAPLCAEPGGIEIDVSEI
                     YLDTTTGVLSGRMRQGFDDKDINRVALIAPENSHVTLSYWPARNCPGGDRPSAPPPPG
                     AEQRSGAPTGAVVVKNDVRVQKFVTRVGPLTVGQHYCLHYEIQSMKGLSDADRQRVDA
                     ALSATISILGEMALGSRANLQRHFAEKLAGSPAREDESFSCTELLATRRLNLEPREFA
                     TLFHCELGWPLNRWVVADPVDGRWTTIRSSIERYINHDKALKRSLSAAWQSRVNAWSA
                     RDEIIESLESGLFNQQTRLPDRDLVYDPLFGVELSALRAALRDPELKRQFQNPKLDTS
                     YAEFAHEVWVQRARLLGPNPKSRNALSPPPVESRLNAFCSSPIEPRIVVVRRAELEAA
                     KASFWSALEQGVPAAQSHIPKLPAASELQKYPPNESDRELIIQLSQNLYAIDTAFERY
                     KRNAERARTELLLSAGYLDFRNHIRELVDSSGAVGFALKTRFAERFPFWVTGDLGVAT
                     AFFASEPKTDPRLGMSLYFGLNFYLTELDKSEPLHITDWRGRDWARRFAIAAGITLTD
                     PSSMEDYGLDGVIGARMLMFGGGLRLTDYVRLNGGGLLYTQRDPNPLSDSARLKVAPY
                     MSASLDVDVIGTVSGWFGKARSSAP"
     gene            8503..10344
                     /locus_tag="VFQ61_11490"
     CDS             8503..10344
                     /locus_tag="VFQ61_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955121.1"
                     /translation="MRRIADRSAVGVLLLLAACSEKAEVTQISAGGAPPAGGTSSGGG
                     SANSGGSSPGGQSSGGATLLPVGGMSSCRTRTCRELGWACGYMLDECGSEKVTNCADE
                     GLSCGTNEVCLGGVDAPTRCVTGGGQDCALCDKIPKCSKENGGEATHLTGRVVTPGRD
                     DANKANQVGVPNALVYITRSADAAELPAVPTGIPSGKTSCDRCEDQELGPVLAGTVTD
                     ATGRFQLDEFIPVGTEFLLVVKAGRFRRAVKYTLPEDAACKTTELPSQLPENPTRLPR
                     SMTDGLAVNIPRIAVSTGQIDAMECVFEKMGLAHGEFGNPTSDGSGARIQLYRGGPMT
                     RPSGARIDAATPHASELYGSLQQLESYDLLVSDCEGQSWDGTFDERRADGAKLREFVN
                     RGGRLFASHLSFSWLHENGTQTYSPDSAIGTGLGPAAEWATMTDMSTTGTGVISVGRP
                     HASPRIANFTDWMVNEGITTEPDHEFDIIEPRSQATALGDSSEEFVFQADGKERVQQF
                     SFNTPYGAPAEAACGRVAYSGFHVSVGGGTSPFGDATFPEHCMGDLTKQEKVLLYMLF
                     DLGACIGVEPPPTCTPATCQSLGASCGFAPDGCGNVLDCGACSGPPK"
     gene            complement(10357..11130)
                     /locus_tag="VFQ61_11495"
     CDS             complement(10357..11130)
                     /locus_tag="VFQ61_11495"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9955122.1"
                     /translation="MQILKTMLLSSGLSIMACGASDASFDQAEMDSRDSPIIGGAVDT
                     GDPSVVAIFSHPRNSYYGSLCTGSVIAPRVVLTAAHCLDPRVAGSGNVVDVYRGTSLN
                     SATRLAVSSVAFDPAFDPDNLDRGHDVGVVILSQATTLKPLPFNRDPNTAPKQGSTVR
                     IVGYGSSTHADSGAGTKRSVSTTVGGVSSSFLLIGGSNQQTCHGDSGGPAFQNINGVE
                     TLVGLTSFGMDRSQTNVCFGGGYDTRVEQVLGFIDKYLQ"
     gene            complement(11486..12442)
                     /locus_tag="VFQ61_11500"
     CDS             complement(11486..12442)
                     /locus_tag="VFQ61_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET9955123.1"
                     /translation="MILLSVPHRIEHRIRAFVSVAAIAIGCARHESQFTTTMPTVGNP
                     AIAFGSGLPKVQPGSGILKLEREMFGRLNRDRAERGLPPLAYDERLAEIGRFHSSDMR
                     DKGFFAHESPTTGALVDRLNRAGYLFITARENLAEAPDVQKGEDTLLASPGHFANIMA
                     PNISHVGIGIVKGGLADASNLLITQVFATPAHIESTAVARSGFIQQIQKQRAERGLPA
                     AAPHAFLNNLANEHIAELGDENRPERVNAVAQKITAVAASSPHNAAQSILVVAQLLPD
                     SSNFEVPGLLLAQKATQFGLAVRRAKSANGRPLLQLLLIVSR"
     gene            12533..13159
                     /locus_tag="VFQ61_11505"
     CDS             12533..13159
                     /locus_tag="VFQ61_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955124.1"
                     /translation="MKVSATSPARDEIVLPFPDAPGVAAYIRSTLLTSSLQSLRARGL
                     EEKHSRLLPPEHRDTILSCVAGQWLPIAVGAAHYEACGKLGLSAAEQHEVGRSVSKQI
                     HETLLGLTVKMARGVGLTPWTLLAKGNTLFSRLFRGGGTQITRLGASSARIEIAGLPL
                     LEIPYFRNAVSGTYAAGIELLGRGASVRLRASDGASFATRCTFFAEWS"
     gene            complement(13184..14515)
                     /locus_tag="VFQ61_11510"
     CDS             complement(13184..14515)
                     /locus_tag="VFQ61_11510"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9955125.1"
                     /translation="MKAVVLSVSPNDRPSALEAQGSLDGALERAGFDERRSFDVGAMT
                     LGFCQGEFDCWLRTPGRCKILDAEQEILASIPAADALVLCGPAAFGGFSWSLKRAIDR
                     LIGLISPFFERRAELTHHRPRYQRYPRLFSVGVGRDISPPERATFDALNDANALNLLA
                     PARSAALLDEAERELWPEQLRGALERQLPAGAGILDRAELRQDLYSAALPDPGFPVRR
                     IRRAALLIGSAKAKGTSASEQLARALARGLAEQGVTCELHFVTEFVHAERGAEAAAQI
                     SRADLFVLASPLYVDAFPALATRALELVAEARASQGQDAAFLPLVNCGFPEPEHTRTA
                     LRIARHFARSARYDFAGALPLGGGGIITPDRSLAEATPPVSHVVRALALTAPALATGQ
                     PVPKAAFNAITSPALPETLYRVMGNWGMRYVAYRNRVAQRELSATPFSSRR"
     gene            14594..15157
                     /locus_tag="VFQ61_11515"
     CDS             14594..15157
                     /locus_tag="VFQ61_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF99 family protein"
                     /protein_id="HET9955126.1"
                     /translation="MTSPITNVIGFDDAPFPTDFRGNVRLVGTICARTRLDGVVSGWV
                     RRDGVNATQCMIEVIRNSQFNGAVRAVLLQGIAVAGFNVVDIHRLCAELAVPVVVVTR
                     RRPRMDRVKTALFHTPRAELKWSWIEAAGEMEKLDHVWVQRAGIGIDETRALIDATTL
                     HGNLPEPLRLAHLIAGGVTTGKSRGRA"
     gene            15243..16679
                     /locus_tag="VFQ61_11520"
     CDS             15243..16679
                     /locus_tag="VFQ61_11520"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HET9955127.1"
                     /translation="MTRQARTFEATSLTCSVLVSALLGCSASEQPILPGGLAGDNGSA
                     FPGQGGNHGVNQGGSIGSPTGTGSSVSTGSPVAGRNAGGATGVGGQGSVARGGAASSA
                     GAAARGGAPAQPSAAGYLHVEGNQLLDDAGHVARFTGVNWFGFETSNQSPHGLWARDY
                     RSMLKQIRDVGFNTVRIPFSDRILRPDAAASSVNTYGADPYDQTDPMNADLVGKTPLE
                     MLDAVVAAARELGLKLILDNHSRKPDGYMEEEVWYSSDVTEEQWIADWVSLAERYRGN
                     PTVAAMDLDNEPHGIATWGTGDAKTDWNSAAERCGNAILEANPDVLIIVEGVAKVGQD
                     GYWWGGNLSGVKTHPIQLSNPKKLVYSAHEHGPEVFDQTWFGSADFPANLAGVWEQHF
                     DFIMKQNMGHILLGEFGLRERDTAQGRSGKWFDTVLTGLAGSYSWTFWCWNPNSGDTE
                     GILGYDWLTPKQWKLDALAPYLAPPIGG"
     gene            complement(16723..17649)
                     /locus_tag="VFQ61_11525"
     CDS             complement(16723..17649)
                     /locus_tag="VFQ61_11525"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HET9955128.1"
                     /translation="MQFASSTNASTAMRRNAPSRAVLVRDMDCKSMKREHGNCNVSTT
                     TAESWEHLQQLLFEGSWDPDLERFRSQFVFRGLDRADYALTTSLMRMGGRYLDIEGHM
                     IRNLRKYASRVVVSRDSDWHWVTVGQHHGLPTRLLDWTYSPFVAMHFATANCRHFQSD
                     GAIWMVHMTDAMALAPPAFQEALKSLGGYGLDLDHLPEVCDSLKSFSAAADSPFVIFF
                     EPPSIDERIINQYAVFSALSDPGLSMDDWLLRPGCEGRVRYKKIVLPAKLKWEVRDKL
                     DHANITERMLFPGLDGLCAWLRRYYGPKNPSQ"
     gene            17673..18215
                     /locus_tag="VFQ61_11530"
     CDS             17673..18215
                     /locus_tag="VFQ61_11530"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9955129.1"
                     /translation="MTFKTVPEPRHDDHARGPAGAAITIIQYGDYACGHTRASHSIVQ
                     RLLAENPDARLVFRHFPLSHLHPDAEELARVTEAAAGLDRFWPVHDALMSQRAAPDET
                     TIQQAVQEARIDLKQLVSRMDDEALLRRVQRDVAEGREAGVHSTPTFFFNGTLHDGKY
                     DYETLRQKLAQARGPKQSDR"
     gene            18326..18784
                     /locus_tag="VFQ61_11535"
     CDS             18326..18784
                     /locus_tag="VFQ61_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9955130.1"
                     /translation="MHPQAAWQLIEVTDSRGEVIEPEWLARAEAVHRQLRPQLPPEYT
                     PALQRVFDGGGRMVVASDGQHVMGLAVYRTQINTFAGFHLYVDDLITDEASRSRGVGQ
                     ALLGHCEARARALGCKLLTLESGVQRQRAHRFYFREGLAIDAYSFSKPLV"
     gene            complement(18948..19274)
                     /locus_tag="VFQ61_11540"
     CDS             complement(18948..19274)
                     /locus_tag="VFQ61_11540"
                     /inference="COORDINATES: protein motif:HMM:NF021593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HET9955131.1"
                     /translation="MTPNPAPTHRRNTVVIVGGKGGLEARYRETVEQNGFDCRYYEDR
                     VPGKLGARNSTVALVIVIVSMVSHPLMARARELAGSAAPIVYLNSPSISAVRQTVTKH
                     LVPAVG"
     gene            complement(19457..21430)
                     /locus_tag="VFQ61_11545"
     CDS             complement(19457..21430)
                     /locus_tag="VFQ61_11545"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HET9955132.1"
                     /translation="MLGVAIGMMSSISAREPALSEQRRTQALLVFPALAASTLSTLVA
                     GWLWLSSAVFLGVVFASVYARRYGSRATALGTIATFAYFDAEFFRAPVSAIPAIVGSV
                     AIGVAIAFVVRFAVVRETPSQLIDSTLACVPLVIRKLVRELAGVCARPERGSRSLGVR
                     RALLRLNEVALTLDEHLQNGGDSGRLPIRLAALDLELAAGQLARQVRASLGQKEDGAG
                     RCQQLMQSLAQFERSLEPSHPPGLDRVAPGELRGVDCSALKLQFDRLMHALARVQELG
                     AARDPRVPSAVPRAAPNGNQPNGNQRIAEPVAVEVNTAAQTPSSIAVSTRQAFQATLA
                     CALAILLGYRLSPDRWYWAVITAFVVFTQVQTVSDTLLRAWHRVLGTLIGVLAGSLLA
                     HVLTGHRWLELGCVFFFIFLGFYLLRVSYAGMVFCITALLAVLYSLLGRSSAGLLELR
                     VLETLVGAAAAVAAAALVLPTPALPRVRTQAAQLLLELEAYLKLALDSQREAIELLES
                     GRKLEADLASLVLAARPLMNRLFVSGRRMAQFVDSLSTLVFVVRQRTNELRGALLVAS
                     QESASRASVNQQIQDISCKLRALSRRAETVSAGRARAVAHTLPLASAGGRVQRDRTSG
                     TGEPSGSAGATSLDRLEQAIVDVDSAARAWQAS"
     gene            complement(21789..22589)
                     /locus_tag="VFQ61_11550"
     CDS             complement(21789..22589)
                     /locus_tag="VFQ61_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955133.1"
                     /translation="MPRFRYVSAFSCVVISALCSLPSHASAQEQAPGDKLQLNKPDLE
                     GKPEAPPAPVSGAPKLVGLSFLAGGLLLDLGDLNDRLEATGHPNSVPGLFPTIGGQGF
                     GLFSHFLIGGSGVGFLPRSTRGAGDTDVSVSGAWGSFDFGYQLIRKNGFLLAPIASIG
                     GYGMTVNVGSRVDTRFDDALRSPGRGTTLTSKGVVGGISILANLILLSRKAPVDTAQS
                     GWALGVRVGGLYGFPYRDWHADNGKASDGPTVGLRGAYAMASLSLGSW"
     gene            23326..>23767
                     /locus_tag="VFQ61_11555"
     CDS             23326..>23767
                     /locus_tag="VFQ61_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET9955134.1"
                     /translation="MARSSLGRVGATIARALVPACLCTCVALPAMAKPWKGAELITRQ
                     TFRYGAFEARIRAARGSGMVTPFFLWKDGSERADQLWQEQDFEIFGRDGSLQTQLMTP
                     GSNDQHRTEHVVNHWLPQAAFERYYTYRMEWTPTRLAFYIDGNLM"
BASE COUNT         4620 a         7526 c         7330 g         4291 t
ORIGIN      
        1 gaactccgag taaggcaact ggtcgacctc gaggcgcaca cggctgccga tctccacgaa
       61 tgctcggtcc ttctctggta aaaaggccac cacatgcttg gccgccccgg tcgaaatcag
      121 cttgcccacc ggcgcacccg ccgtgatgac gtcccccgcg cgcaccagta ccgcttctac
      181 gtacccgtcc tgcggcgcct gaatcaaggt ctgctgaagg gagaaggcga gtgcggttcg
      241 cttggtctct gcttcttgaa tctgctgctc cgccttccac agctcggatt ggtgtgccga
      301 attgagcgta gccaattcga ggctcgtctg ggacaggctt tcatcgagct cgatgcgctg
      361 gcgccgggct cgatcgagtt cctcgacgac ctcgtcgcgc gcgatcgcgc cgaccatgcc
      421 tttgtcgttc agcgcgttgt aggacccaac tttcgcctcg aggcgtgcaa tcgtgttctc
      481 ctggctcgcg actcgctgct tcaggagctc cgcgtgactg cgcagcagcg ccgtacgcgt
      541 gcggtgaatc tcctcttgtt tggtcgtaaa ggacttcaag ttggagcgaa ccagctcgag
      601 ttgacgatct gcctccaaca gggccgcctg tactccggcg gattcgagct tcaccaaggg
      661 ctcaccgacg agaacgctct gccccgactt ggccgagacc tcggtcacga ccccgccgac
      721 ctgggtctgc agagttctca ctccaccggg tgcacgaaga acacccttgc cacggctcgt
      781 gagctcgact tcggccacga atgacacgac gaccgtggac gcgacgatcg caaccagcat
      841 ccagaacaga gtccaagacc aaggcggcac gacccgcagc acttgtccgt cgatctcgag
      901 ttcgcgggtg aggtgatgat ccagcgcctc ccgtctgaat agcgaattct ttttgggtga
      961 ctgttgctgg gcgttggcaa ttgatgacga agcactcacg ttcagctaag aatcagcgta
     1021 ctcgcagctt ggtgctgccg tccataggac accacgcgtg ggacccctgc gcacgcaagc
     1081 aatcacctat gaagatatgc atattcttgg gccctagtct ggcaatcgag gaagcgcgcc
     1141 gcctgctgcc cgaggcgaca ttcctgcctc ccgcgaagat gggagatatc tgcacgctcg
     1201 cttcgagcca cgacccgcca gacgtaatcg gcgtaatcga tggtgtgttc gagcaagtgc
     1261 cgtccgtttg gcacaaggaa atcctattcg cgatctcccg tggaattcag gtttatggtg
     1321 cgtccagcat gggtgcgttg cgagcggcgg agctgcatcc attcggaatg cgcggcgtcg
     1381 gacgcgtatt cgaagcgtat gcgtgcggag aatacgagga cgatgacgaa gtggccgtcg
     1441 tgcatgcggc tgcatccgcc ggtcacaagc ctttatccga agccatggtc aacatccggg
     1501 aagggctgag gctcgcgcgt caagacggtc taatcgacga cccgacccac caatcactca
     1561 ttgacatggc caagggtcag ttttaccccg agcgctcgtg gcgcggactt ttccgcctgg
     1621 tctcccagag cgggatcgcc aaggaacggc tgacacgact ggccgagttc attgccgagc
     1681 gcgatccgaa catcaagcgt gcggacgcga tggcactgct cgaacgtgtt cgcgcggacg
     1741 ttgccaccgc ggccccgccg tatgagcctg actatcagtt ctcctctacc gtgttttgga
     1801 agaagctgca gcagagtgct tcgagtcatg ggccttccat tggagcgctg ggccaggtcg
     1861 caaacggcgc cgcggctcaa aacgcggtgc tgattcacgc gaaggtcgct gagccgcgct
     1921 ggcgagaagt tcaacgtgat ggcctgctga gggtgttgat tcaagacgag acgaggcgcc
     1981 gtggcttgga ggctgacgag cagcgcatct cagccgccgc ccagagtttt cgccgagagc
     2041 gcgggctcgt gagcggcgag gcaacgcgcg catggctcac cgagaacgag ctctcgcgag
     2101 aagagttctc gtctttgatg gagattgagg ctctgtacga gttggttctc gaacaggagc
     2161 gagcgcgggt ggatcagatc gcaatctccg aattgaaacg cgccggtcga ctcggagagc
     2221 ttcgacgcga agtgaacgtc aagatcagtc acctcgcgca acttgggatc gacggcccga
     2281 acgtggaaga cacgggtaac tcccccaccg aactcctgaa gtggtacgag acacagttcg
     2341 gagctctgca gtgcccgttg agcgaacacg cgctgcggat cggatttgcg agctccgccg
     2401 acttcctgcg tgctgtggcg gagctttact tggcagagcg ccaagccatg cgtacggcag
     2461 cggccgagtg atcgagctgt gagtgacgcg agagcacgat gtcggttccg cgctctcctc
     2521 cactcgctgg gtcttcagct cgctgggtct tcagctcgtt aggctggagc tcgcgtgggc
     2581 ttcagctcgc gtgcgtggaa ttgaaccggc gttggccaac agccatgagc acccaggaca
     2641 ccgccagaaa ggagaaccca agaatggctg gcgcgaggga ctcggcgata cccgacagca
     2701 ccagcaaatg tatttgaggg ctctgtgccc actcggggcg agtagacacc tgggagccca
     2761 cggtagccaa gttcaaggct acgccggaca cgattgcgca tattgtggcc ctcgagaaga
     2821 atgtcaccac ctgttcgcgc gcggaatggg ggtgacgcgc aaaccccccc gccgccgcca
     2881 acgtgatgat cccgagcagc aggacgaacc acatcgacca accaccttct tggaagaaca
     2941 accagatcct catcgcaccg tactcctatc tccgtctgct attcaacgtc gcctgtcgac
     3001 tcaacgtggc ctctcgactg aacgtcggct gtggacccag gctcatccac ggaagctcgt
     3061 ggccgagcgt gctccggcct caccccacac gacgctcggt cctggccagc gtgacgaact
     3121 tcaatcattt tccctgttcg cccgaggcat gctgattttt ctgtcacgcc aacgcgcgcc
     3181 gagcgtcgat cgcagcgtgc acgaaccagc accggcagcc cctgggaggc ctgcgccacc
     3241 tttcggggag gccgcggaac cggacgtgcc cgatacgcac gccaccgcac gcgccctgga
     3301 agccgccgcg atgcccgagc cacacgcagg taccacagag ctgccaggtg aagaatccgc
     3361 cgaggcctcg cgtgaaccgg cggactcact caccatcgca gctctcgcgc gcggagaccg
     3421 gcgcgccttt gatgccgtct acgagcaaca tcgcgcacca ctctatactt tcctgttgcg
     3481 tctcagcggt cgagcagctc tggctgaaga ccttctgcag gagacctggc tgcggctcgt
     3541 ccatcacgcg gccgcgcttc caccgggaac tcagcttcga ccgtggctat tcaccgttgc
     3601 ccgcaatttg cacaccagcc ataggcgctg ggcgctgctg gatgggtcgc ggctactgga
     3661 cctcgcattt cagccccgcg gctcgagtcc ttcgccgctc gaagcgatgg ctgccaccga
     3721 ggctcagcgc gcgctcgaac gcgccttgac caggctcccc ctggcagacc gcgaggcgct
     3781 gctgctttgc gtcgtctccg gattcgagcc ctctgaggcg gcgtccatct tgaacatcag
     3841 cgccgaagcg acccgtcaac gcttggcgcg agcacgagcc aaactcaaag tactcctgag
     3901 aagtaacgag gcgtccgatg aaccctgaac tcgagttgcg gaaactgccg tctcgtgacg
     3961 tgcccgaaca tgtgactgcc caaatccgcg agcgagccta cgcggcgcta cgagaaaaag
     4021 cgcggagagg aggtccggac ggggcgccag ctcgtttttc ctcgcaccgg ctatttggtg
     4081 agactgcact gctgttcgcg gcaagccttt tgtacctgct gggaaccgtc cgcaatacaa
     4141 tctacttatt cccgtgaaga cagcctagag gccatcgcga ttggagcgct ggagagcacg
     4201 gcgacatcga gtttctgaaa ggggcggcac tagttctggc gcagcaagac gctggccgag
     4261 agcgaagcgg ctcagtttct ctccatgtcg gtagccttgg tagagaccct gtgttgtccg
     4321 aagctcgcca acgtggcatt aattccaagt tcgaatcggc tgcggccggt cccccaacgt
     4381 caaggactca cggttgggcc gggcgggcga gcgcgagtgc gatctgctgc caggccgcaa
     4441 ctctgcgtgg tggctcgttt tgccaatccg tgcaggcgat tgaggggctc ccctcctcca
     4501 tcacgaacgc gaacagggtc acggccacat tcttttgctg cccagaggcc gcggtctgta
     4561 cgagattggt gccagcgggg gtgagagcgg caatcgcgct ctctcgccca tccccgtcta
     4621 cgtcttggac gaagcttggc accgctagat agcgagtgca gtcctccaag ctcccggaat
     4681 cgatctcaca acatgggtgc agccatatcg tggttccggc cgatacgagg ccccgtccgg
     4741 cgtctcgcgt caacgtggac ctaatctgaa gctgagagtg gctcgagtcc aacacgctgc
     4801 catccaagag ctccaaggcg attcggtccg gtaacgcggg ctgcaaccac agcgagaccg
     4861 ctcgatccgg agtcgtcccg ctgtgggccg tgagtagggc tggccccggc tgccgaccga
     4921 cactcaggtt caccagcgcg gtttcctgtc cgcgaaccgt aatccgcttg gtgcgcctct
     4981 cttccggcgt cgtacccgcc gcgttcaact cgccgagagt ggtcgtgagc gtgaccgtct
     5041 ctagctcgcc gagcggcgcc gcgtaatcca agagaatcgg cacgaccgtt tggtcgtcgg
     5101 ccagtgctga ctcgggcgct ccgatcgtga gatctcgcaa ggcttggtcc aactgtacga
     5161 tctccgtctc tagtacgttg cccagctcgg cggagaccac ggcgttcccc gcagagttcc
     5221 ccaagatcca cgtcaagtct cggggtcgat tgggggccac tcgaaccgta cgcgcgcgag
     5281 ccgcttcgtt ggctttgtcg ggtgagagta tgccgtgcga ggtcttcacg agcacatctc
     5341 ggaaggcggg actgccggat gtgaggccaa tggtcaaggt cgtttcgtgg gcgccatccg
     5401 cgatgtatgg gcccgcagag accaagatgg agagttcgtc gttcaccggc tccaagacga
     5461 agtctgcgtc ttggcggagt ccacccggcc cctccacggt gatccggccg acacccgcga
     5521 cttgctcggc atacaagacg aagtcgatcg gggagccatg gctcgtctgc agttcgagcc
     5581 tgcgggtgcc cgcatcgtcc gccgctgggt tgatgatacc gagtgatgtc gtggcgcgga
     5641 gcggcttgcc cgccgtgatc ctatgctcga agctcacccg tacgatacgc cggctcattc
     5701 cgtccgcgta gaagcccgcc ggctccagca gttccacgcg cagctgcccg tcgccgcggg
     5761 actcgaagcc acagccccac gagagcgttg ctagcagcgc aggcgcaatc aaccctatcg
     5821 gtttcatgag atcctgacga ttggaaatgc gggggaacgc gcggtctgct ccgcaaacag
     5881 ccagactcct ccgtccgtgt tcgcctcccc acaaccatcc tcgttgtcct tggagcacca
     5941 ggtgcccacg gcgcgcacct cgaggcctag ctcgtcggcg ctagccaaga tgcgccccga
     6001 cgcggcggcg ctgccgtccg gccgcactcg cacctcgtat acgccggggg ttttgagatt
     6061 tgcttccagt attctcggca tgattgaacc tcaaggcgcc gaacttcggg ccttaccaaa
     6121 ccagccactg acggtgccta ttacatcgac atccagcgag gcgctcatgt acggggctac
     6181 cttcaaccgc gcggagtcgc tgagtggatt gggatccctt tgggtgtaga gcaagccgcc
     6241 accgttcaga cgtacgtagt cggtcaagcg caggccgccc ccgaacatca acatacgggc
     6301 cccgatcacg ccatcgagcc cgtaatcctc catgctggat gggtcggtca aggtgatgcc
     6361 ggcggcgatg gcgaagcgcc gcgcccagtc gcgacctcgc caatccgtga tgtgtagggg
     6421 ctcggattta tcgagctccg tgagatagaa attcaacccg aagtacaggc tcatgccgag
     6481 ccgtggatcg gtcttcggct ccgaggcaaa gaacgcggta gcgaccccca aatcgccggt
     6541 cacccaaaaa gggaaccgct ccgcgaatcg agtcttgaga gcaaacccca ctgcgcccga
     6601 agagtcgact agttcccgaa tatgatttct gaagtctagg tagcccgcgc tcagcaacaa
     6661 ctccgttctt gcccgctcgg cgttgcgttt gtagcgttcg aacgccgtat cgatcgcata
     6721 caagttttga ctgagctgaa tgatcagctc gcggtcactc tcgttcggag gatacttttg
     6781 cagctcggaa gctgcgggca gtttcgggat gtgcgactgg gccgcgggca cgccctgctc
     6841 gagcgccgac cagaagctgg ccttcgccgc ctccagttcc gcgcgcctca ctacgacgat
     6901 gcgtggctcg atgggactgc tgcaaaatgc attcaggcgt gactcgacgg ggggtgggct
     6961 cagcgcattg cgcgacttgg gattcggacc cagcaagcgt gcgcgctgca cccagacttc
     7021 gtgggcgaac tcggcatagg acgtatcgag tttcggattc tgaaactggc gcttcagctc
     7081 tggatcgcgc agcgcggctc gcagtgccga gagctccact ccgaacagcg gatcgtagac
     7141 caagtcccga tccggcaagc gggtctgctg attgaataaa ccactttcga gtgattcgat
     7201 gatctcgtcc cgcgcagacc acgcatttac gcgcgactgc caggccgcgc tcagtgatct
     7261 cttgagcgcc ttgtcgtggt tgatgtagcg ctcgatcgag ctgcggatcg tggtccatct
     7321 accgtccacg ggatccgcga ctacccagcg gttcaaaggc catccgagct cgcagtggaa
     7381 taaggtggca aactcgcgtg gctcgaggtt cagcctgcgc gtagcgagca gctcggtgca
     7441 ggagaatgac tcatcttcgc gcgcaggcga gccggcgagt ttctctgcga agtgccgttg
     7501 caggttggcg cggctcccga gcgccatctc tcccagaatc gagattgtcg cactcaaggc
     7561 tgcgtccacg cgctggcgat cggcgtcact cagccccttc atggattgga tttcgtagtg
     7621 caagcaataa tgctgaccga ccgtgagggg ccccacccgg gtgacgaact tctgcacgcg
     7681 aacgtcgttt ttaacgacca ccgctccggt gggcgccccg ctgcgttgct ctgcgccggg
     7741 cggaggcggc gcgctgggcc gatcgccacc cggacaattt ctggcaggcc aatacgataa
     7801 cgtgacgtgc gagttttcgg gcgcgatgag cgcaacccga ttgatatcct tgtcgtcgaa
     7861 gccctggcgc attcgccccg agagcacccc cgtcgtggtg tcgagataga tctctgatac
     7921 gtcgatttcg atgccacccg gctctgcgca taaaggagca ccgctccaga aaacttgagc
     7981 cgacaccagc gcgacgccca gtgaactccg cccgcgcatt ctccaagcca aagaaggcat
     8041 gaggagccgc tcaatgtagc cctcccgcgc agctgcactc aataggcatt caggccccac
     8101 gcttcagatg gctgtcattc tcgaagccat ctcacattca accactccgt accgagagcc
     8161 cctccggcga ctaaccccta tggggatgga tattgggtcg ggctgagtcc aacccgagcg
     8221 cgtcgccgcc cgcaccgccg agcgcgttgg gttgcccggc gcgttggcgt tggccccaca
     8281 cttgttgctc cgctttcaca gcagcccggc agccgagggc ttcaccttcg ggtttgggtt
     8341 acgggcaggt gtcgagaccg cgatctgaac gaaaaccgct tcgatggctt gttcttttgt
     8401 actcgagcgg tagccgttcg aagggtgctt cctcgaaatg ttacgcaagg gtgagctact
     8461 tcgatggctt ttccccaccg aggagtcaaa ggtagaccat atatgcgtcg tattgctgat
     8521 cgttcggccg taggggtcct ccttctcctc gctgcctgct ccgagaaggc cgaagtcacg
     8581 caaatcagcg cgggcggggc gcctccggcg gggggcactt cgtctggcgg aggttccgcg
     8641 aactcaggcg gctcgagccc gggtgggcag tcgagcggcg gggccacgct cttgccggtg
     8701 ggcgggatgt cgagctgccg tacccgaacg tgccgtgagc tcggctgggc ctgcggatac
     8761 atgttggatg agtgcggcag tgagaaggtc accaattgcg ccgacgaggg gctgagctgc
     8821 gggaccaacg aggtgtgtct cggcggcgtc gatgccccta cgcgctgcgt gaccggcgga
     8881 gggcaggact gcgccctttg cgacaagatc cccaagtgct ccaaggagaa tggcggggag
     8941 gcgacgcacc tgaccgggcg cgtagtgacc cccggccggg acgacgcgaa caaggccaac
     9001 caggttggag tgcccaacgc actcgtgtac atcacgcgca gtgcggacgc agcggaacta
     9061 ccggcggtac ccacgggcat tccgagcgga aagactagtt gcgaccgctg cgaggaccaa
     9121 gagctcggcc cggtcctcgc aggcaccgtg acggatgcca cgggtcgctt ccagttggac
     9181 gagttcatcc ccgtgggtac ggagttcttg ctggtggtca aagcggggcg gttccggcga
     9241 gccgtcaaat acacattacc ggaagacgcg gcttgcaaga ccacggagct gcccagccaa
     9301 ttgccagaga atcccacacg cctgccacgc tcgatgaccg acggccttgc cgtcaatatc
     9361 ccgcgcattg ccgtatcgac ggggcaaatc gatgccatgg agtgcgtgtt cgaaaagatg
     9421 ggcttggcgc acggtgagtt cggcaacccc acttcggacg gcagcggggc gcgcattcag
     9481 ctctatcgcg gcggcccgat gacgcgccca tcgggcgccc gcatcgacgc ggcgacgccg
     9541 cacgcttccg agctctacgg cagtctccaa caactcgaga gttatgatct gctggtctcg
     9601 gactgtgagg ggcagtcctg ggatggcact ttcgacgagc ggagagcgga cggcgccaag
     9661 ctccgagaat tcgtgaatcg tggcgggcgg ctatttgcaa gccacttgag cttcagctgg
     9721 ctgcacgaaa acggcaccca aacctactcc ccagattcgg cgatcgggac cgggctgggc
     9781 cccgccgcgg agtgggcgac catgaccgat atgtccacga cggggacggg agtgatctcc
     9841 gtgggtcggc cccacgcgag cccgcggatc gcgaacttca cggactggat ggtcaacgag
     9901 ggcatcacga ccgagcccga ccacgagttc gacatcatcg aacctcgcag ccaagccacg
     9961 gcgctcgggg attcttcaga agagttcgtg tttcaagcgg atggcaaaga gcgcgttcaa
    10021 cagttttcgt tcaatacgcc gtacggcgcg cccgcagagg cagcctgcgg gcgcgtcgcg
    10081 tacagcgggt tccacgtctc ggtgggtggc ggaaccagtc cttttggcga cgccacgttc
    10141 cccgaacact gcatgggcga cctgacgaag caggagaagg tcctgctgta catgctgttc
    10201 gaccttgggg cttgcattgg ggtagagccg ccgcccacct gcacgcctgc gacttgccaa
    10261 agcttgggcg cgagctgtgg attcgccccg gatggctgcg gcaacgtgct cgattgcggc
    10321 gcctgttccg gccctccgaa gtagagccca cgccctctat tgcagatact tgtctatgaa
    10381 cccgagcact tgctctacac gagtgtcgta cccaccaccg aaacagacgt tcgtctgtga
    10441 acgatccata ccgaaagacg tgagaccgac cagggtctcg acgccattga tattctggaa
    10501 cgcggggcca ccagagtcgc cgtggcaggt ttgttggttc gatcctccga tcaacaagaa
    10561 cgaggaggac acgcctccga ccgtggtcga gacggatcgt ttggttccgg cgccgctatc
    10621 cgcgtgcgtg ctcgagccgt aacctacgat gcgcacggtc gaaccctgct tcggggccgt
    10681 gttcggatcg cgattgaagg gtagcggttt gagcgtcgta gcttgcgaca ggatcaccac
    10741 tcccacgtcg tgacctcggt cgagattgtc tggatcgaac gcgggatcga acgccaccga
    10801 ggatacggcg agccgggtcg cgctgttgag agaagtgccg cgatagacat ccacgacgtt
    10861 tccactcccc gcgacccgcg ggtccaaaca gtgagccgcc gtcaacacca cacggggggc
    10921 gatcacgcta ccggtgcata gggacccata atatgagtta cgaggatgac taaaaatggc
    10981 aaccacggaa gggtcaccgg tgtctaccgc gccgccgatg atcggtgagt cgcggctatc
    11041 catctcggcc tgatcgaagc tcgcgtcgga agcgccgcaa gccatgattg agagcccact
    11101 cgacaacaac atggttttca gaatctgcat gtatcgactc ggacaccgaa tcaccctcga
    11161 agattggcaa aaatcgtttg tgcaattttc gccgattgac cgctcccaca cacacctcct
    11221 agggattacg aacgagctaa caaagcaagc gaccgctccg gattcaggaa cacgagatgt
    11281 ggattcgagc gggtaaacac aagcccaccg cgtcaaggct ttggccgcca acccacgaat
    11341 tccgacgagg tctcgagcat aagagtggcg agcccctgaa tggacatcga aggctgcaga
    11401 ccctagggga tgaacgaccg ttcgagtttc gccgccttcg cagcttgggc ctaccgatac
    11461 gggcctgtcc gatacccgat ctcgatcagc ggctaacgat cagcaggagc tgcaacaacg
    11521 ggcgcccgtt ggcgctcttg gcgcggcgca ccgcgaggcc gaactgagtc gccttttgcg
    11581 ccagcaacag gcccggaact tcgaaattcg aagaatccgg gagcagctgc gccacgacca
    11641 gaatcgactg cgcggcattg tgcggggagg aagctgcgac ggccgtaatc ttctgagcca
    11701 ccgcgttgac gcgctcgggt cggttctcgt ccccgagctc cgcgatgtgt tcgttggcga
    11761 ggttgttcaa gaaggcatgc ggcgcggccg cgggcaggcc gcgctcggct cgttgcttct
    11821 gaatctgctg aatgaagccg ctccgcgcca ccgcggtcga ctcgatatgt gcgggcgtcg
    11881 cgaacacctg agtgatcagc agattgctcg catcggccag tccgcccttc acgatcccaa
    11941 tcccgacatg gctgatgttg ggcgccatga tattggcgaa gtgcccagga ctcgcgagca
    12001 gcgtgtcctc tcccttttgc acgtctggcg cttcggccag gttctctcgc gcagtaatga
    12061 acaggtagcc agcacgattc agccgatcca cgagagcacc cgtagtgggt gattcgtgcg
    12121 cgaagaagcc cttgtctcgc atatccgagg agtgaaaacg cccaatttcc gcgaggcgtt
    12181 cgtcgtaggc gagcgggggt aagccgcgct ctgctcgatc acgattgagc cgcccgaaca
    12241 tttctcgctc cagcttcagg atgccggacc cgggctgcac cttcgggagc ccactaccaa
    12301 aagcgatggc agggttaccc acggtgggca tcgtggtggt gaactgactc tcgtgacggg
    12361 cgcagccgat cgcgattgcg gcgacgctga cgaaagccct aatccgatgc tcaatccggt
    12421 gcggaaccga aagcaaaatc acgccgaagc gtacccgatc gccctcgcgt ccaccatatg
    12481 ggcaaacgtg ccaccgttac caggctcgag aggctggccg tctgggatgg ggttgaaagt
    12541 cagtgccact tctcccgctc gcgatgagat cgtgctcccg ttcccggacg ctccgggggt
    12601 ggccgcatac attcgaagca ccctgctcac gtcttcctta cagtctctcc gagccagagg
    12661 cctcgaggaa aagcatagtc ggctcctccc ccccgagcac cgggacacca tcctgagttg
    12721 cgtcgcgggc cagtggctgc cgattgcagt cggggccgcc cactacgagg cgtgtgggaa
    12781 gctggggctg agcgcggcgg agcagcacga agtcgggcgc agtgtctcca agcaaattca
    12841 tgagacgctg ctcgggctga cggtgaaaat ggcacgcggc gtaggcttga cgccttggac
    12901 gctgctcgcc aaaggcaaca cgctgttctc acggctcttc cgtggcggcg ggacgcagat
    12961 cacgagactt ggcgccagca gcgcgcgcat cgagatagcg gggctcccgc tgctcgaaat
    13021 cccgtacttc aggaacgcgg tgagcggtac ttatgcggct ggaatcgagc tcctgggtcg
    13081 cggagcctcc gtccgcttgc gtgcttcgga cggagccagt ttcgcgacgc gctgcacgtt
    13141 cttcgctgag tggtcctagg gcccgcgtac gacctacctg caattagcgg cgcgacgaaa
    13201 agggtgtggc actcaactcg cgttgggcaa ctcgatttcg atatgccaca tagcgcatgc
    13261 cccagtttcc catcacgcga tagagcgttt cgggcagcgc gggcgaggtg atggcattga
    13321 atgccgcctt gggcaccggt tgccccgtgg ccagcgctgg cgcagtgagg gcgagcgcgc
    13381 gaaccacgtg agacaccgga ggcgttgcct ccgcgaggga gcgatcgggc gtgatgattc
    13441 ctccaccgcc gagagggaga gcgccggcaa agtcgtagcg tgccgatcgc gcgaagtgac
    13501 gggcgatgcg cagcgccgtg cgcgtgtgct cgggctccgg aaagccgcaa ttcactagcg
    13561 gcaggaaggc cgcgtcctgg ccctgacttg cgcgcgcttc agccacgagc tccaaggcgc
    13621 gcgtcgccaa agcggggaag gcatcgacgt acagcggcga tgcgagcacg aacagatccg
    13681 ctcgtgagat ctgcgccgcc gcctccgcgc ctcgctcggc gtgcacgaac tcggtcacga
    13741 agtgcagctc gcacgtcacg ccctgctcgg cgaggccacg cgccaatgcc cgcgccagtt
    13801 gctcggaagc gctggtcccc ttggctttgg cgctgccaat cagcagcgcc gcgcggcgaa
    13861 tccgtcgaac cggaaagccc gggtccggca aagccgctga atataagtcc tgtcggagct
    13921 cggctcggtc cagaatcccc gccccggccg gcagttgacg ctcgagtgcg ccccgcagtt
    13981 gctcgggcca cagctcgcgt tccgcttcgt ctagcagcgc agcgctacgc gcgggtgcaa
    14041 gtaggttcag agcgttggca tcattcaggg catcgaacgt ggcacgctct ggaggactga
    14101 tgtctcgacc cactccaacc gagaacaagc gcggatagcg ttgataacga gggcggtgat
    14161 gggtcagctc ggcgcgccgc tcgaagaatg gtgagataag cccgatcaaa cgatcgattg
    14221 cacgcttgag gctccaagag aacccgccga acgcggccgg gccacacagc accagcgcgt
    14281 ccgcagccgg aatcgaggcc aggatctcct gttcggcgtc caagatcttg caacgccccg
    14341 gcgtcctcaa ccaacaatcg aactccccct ggcagaagcc gagcgtcatt gcccccacat
    14401 cgaagctccg tcgttcatcg aagcccgctc gctccaaagc accgtccagg ctgccctggg
    14461 cttcgagcgc gctgggccgg tcattcgggc tcacggagag gacgacggct ttcatgacta
    14521 ctctgactta acgactgtaa ccactccgag caaccggcgc gctcgactca cctaacaagc
    14581 tacaatcgga gacgtgacgt cccccatcac caacgttatc ggtttcgatg acgctccctt
    14641 ccccactgac ttccgcggca acgtgcgatt ggtcggcacg atctgtgcgc gaacacgact
    14701 cgacggcgtg gtctcgggct gggtgcgtcg tgatggcgta aacgccaccc agtgcatgat
    14761 agaggtgatt cgaaacagcc aattcaatgg cgcggttcgg gcggtgctgc tgcaaggcat
    14821 cgccgtcgcg ggcttcaacg tggtcgacat ccacaggttg tgcgcggaat tggcggttcc
    14881 ggtggtggtc gtaacgcgac ggaggccgcg catggatcgg gttaaaaccg cgctattcca
    14941 tacgccgagg gcagagctca agtggagctg gatcgaggcc gccggtgaga tggagaagtt
    15001 ggatcacgtt tgggtgcaac gggccggaat cgggatcgac gagactcgag ctttgatcga
    15061 cgccaccacg ttgcatggca acttgccgga gccgctgcgc ctcgcacacc tgatcgcggg
    15121 gggcgtcact acgggcaaga gccgtgggcg agcctaacgc cgatcagccg cgcggtgctt
    15181 actaccctcc gggatgatgc cgctcatgcg cggcctcgaa gaaatggggg agcctgtagc
    15241 ccgtgaccag acaagcgcga acgttcgagg ccacatccct cacttgctcg gttctagtct
    15301 cggcgctgct cgggtgcagc gcgagcgagc agcctatttt gccgggaggg ctcgccggag
    15361 acaacggcag tgccttcccg ggccagggag gcaatcacgg cgtcaaccag ggcggctcca
    15421 tcggtagtcc aaccggaacc ggctcttcgg tgagcacggg gtcgcccgtc gcaggccgga
    15481 acgcgggcgg cgctaccgga gtgggcggac aaggctcggt cgcccgcgga ggcgccgcaa
    15541 gcagcgcagg ggccgcggcg cgtggcgggg cgccggctca gccatcggcg gcaggatatc
    15601 tgcacgtgga aggcaatcag ctgctcgacg acgcgggcca cgtcgcgcga ttcacgggtg
    15661 tgaattggtt cggattcgag acatcgaatc agtcgccgca tggtttatgg gcccgtgact
    15721 accgcagtat gctgaaacag attcgggacg tcggattcaa caccgtccgt attcccttct
    15781 cggaccgcat cttgcggccc gacgcggcgg cctcttcggt caacacctac ggggccgatc
    15841 cgtacgatca gacggacccc atgaacgccg atctggtcgg gaagacgccc ctcgagatgc
    15901 tcgacgccgt cgtggcggcc gcgcgagaac tcgggttgaa gctgatcctg gacaatcact
    15961 cgcgcaaacc ggacggttat atggaagaag aagtctggta cagcagcgac gtgaccgaag
    16021 agcaatggat tgcggattgg gtgtcgctcg cggagcgcta ccgtggcaat ccaaccgtcg
    16081 cggccatgga tctcgacaat gagccccacg gcattgcgac ttggggcacg ggtgacgcca
    16141 agacggactg gaactccgcg gccgagcgtt gcggcaatgc cattctcgag gctaacccgg
    16201 atgttctaat catcgtggag ggagtcgcga aggtcgggca agatggctat tggtggggcg
    16261 gcaacctgag tggcgtgaag acgcacccga tccagctctc gaaccccaag aagctcgtct
    16321 actcggcgca cgagcatggg cccgaagtgt tcgaccagac ttggttcggc agcgccgact
    16381 ttcccgcgaa cctggccggg gtctgggaac agcacttcga cttcatcatg aagcaaaaca
    16441 tggggcacat tctcctcggc gagttcgggc tgcgcgagcg agataccgct cagggccgct
    16501 ccggcaaatg gttcgacacc gtgctcacgg gcctcgcggg ctcgtactcc tggacgtttt
    16561 ggtgttggaa ccccaactcg ggtgataccg aaggcatcct cggctacgat tggctcactc
    16621 cgaagcagtg gaagttggac gcgctcgcgc cctatctggc cccacccatc ggcggttgaa
    16681 gagagctccc tgttacgcgc aagctcggcg ctacgcccga acctattggg acgggttctt
    16741 cggaccgtag tagcgccgca gccaagcgca caagccatcc aagcccggga agagcatgcg
    16801 ttcggtgatg ttggcgtgat ccaacttgtc acgcacttcc cacttcagct tggccggcaa
    16861 gacgatcttc ttgtagcgaa cgcgcccctc acaacccggc cggagcagcc agtcatccat
    16921 gctcaagccc gggtccgaga gtgcggaaaa aactgcgtac tggttgatga tgcgttcgtc
    16981 gatcgagggc ggttcgaaga agatgacgaa cggcgaatcc gcggctgcgc tgaaagactt
    17041 cagactgtcg cagacctccg gtaagtggtc gagatcgagc ccatagccgc ccagggactt
    17101 cagcgcttct tgaaaggccg gcggggcgag cgccatcgcg tccgtcatat gcaccatcca
    17161 aatcgccccg tcggactgga agtgtctaca attggcagtc gcgaagtgca ttgcgacgaa
    17221 cggcgagtaa gtccagtcca acaagcgtgt cggcaagcca tgatgttggc ctacggtcac
    17281 ccagtgccag tccgagtccc tcgaaaccac gacccgggac gcatatttgc gcaggttccg
    17341 gatcatgtga ccttcgatat cgagatatcg cccacccatg cgcatcagac tggtcgtgag
    17401 cgcgtaatcg gccctatcca gacctcggaa gacgaactga gatcgaaagc gctcgaggtc
    17461 aggatcccaa gatccctcga acaatagctg ttgcaggtgc tcccaacttt cagccgtcgt
    17521 cgtagagaca ttgcagttcc cgtgctctcg tttcatcgat ttacaatcca tatcacgcac
    17581 gagcacggct cgggatgggg cattcctgcg catggcggtg ctggcattgg tcgagctcgc
    17641 gaattgcatg gcggccttcg tcggagaagc caatgacctt caaaacggtt cctgaaccac
    17701 ggcatgacga ccacgcacgc gggcccgccg gggctgcaat caccatcatt caatatggtg
    17761 attatgcctg tgggcacacc cgcgcctcgc actcgatcgt acagcgcttg cttgcggaaa
    17821 acccggacgc caggctcgtc tttcgccact ttcccctgag tcatttgcac ccagatgcgg
    17881 aggagctggc gcgggtcacc gaagccgccg caggtctgga tcgcttctgg cccgtgcacg
    17941 atgctttgat gtcccagcgg gcggccccgg acgaaaccac catccaacaa gcggtccagg
    18001 aagcacgaat agacctgaag cagctcgtgt ctcgaatgga cgacgaggcc ttgctgcgtc
    18061 gagtacaacg cgacgtggcc gagggtcgcg aggcgggcgt gcacagcacg ccgaccttct
    18121 tcttcaacgg gacgctgcac gacgggaagt acgactacga gacgttgcgc cagaagctcg
    18181 cgcaagcacg tggaccgaag caatccgacc gttgaactcc gggtgtgagt cgccggtgaa
    18241 agtcgctgcc atcgagcggc taggccagag cgcggctgcg cgagccactt gacggattga
    18301 cccgatggtc tgctaaatcc gaggcatgca tccccaagcc gcctggcagc tcatcgaggt
    18361 gacggactct cgcggtgagg taatcgagcc cgagtggttg gcgcgtgcgg aggcggtaca
    18421 tcgccagttg cgcccgcagt tgccgcccga gtacacacca gccctccagc gcgtgttcga
    18481 tgggggtggg cgaatggtcg tggcgagcga tggccagcac gtaatgggcc tggcggtcta
    18541 tcgcacgcag atcaacacct ttgcgggctt tcacctgtac gtcgacgacc tgatcactga
    18601 cgaggccagc cgctctcgtg gagttgggca agccctactc ggccactgcg aggctcgagc
    18661 gcgagccctc ggttgcaaac tactcacctt ggaatccggt gtgcaacgcc aacgtgcaca
    18721 cagattctac tttcgcgagg gcctcgcgat agacgcttat agcttcagca agcctctggt
    18781 ctaatgcacg ttggccattg cgccctgcgt aagtaacgcg tgtccggtcg ctcgtcaccg
    18841 acgctctcga aattcaccca tgcgagctat gatcattctg agcactcaat ctgacggccc
    18901 ctccgaaccg gcttgcgtcg ggagcgtcaa aaccaaacag aaccgagcta gccgacggca
    18961 ggcacgaggt gcttggtgac ggtctggcga acggccgaaa tggaggggct gttgaggtat
    19021 acgatagggg ccgcgcttcc cgcgagctcg cgcgcgcggg ccatcaacgg atgcgaaacc
    19081 atggagacaa tgacgatcac cagggcaacg gtgctgtttc gggctcccaa cttgccggga
    19141 actcgatcct cgtagtagcg gcagtcgaag ccgttctgct ccacggtttc tcgatagcgt
    19201 gcttccaagc cgcctttgcc gccaacgatc acgaccgtgt tacgcctgtg ggtgggtgcg
    19261 ggattcgggg tcatgcaccc gagcatcgca agccgcgcgc cagcgcccgt tgcccgcggg
    19321 gaccgcagga ttttcgctga ttcatggctt ggcgcttcta ttccccgcca tgttgacgcc
    19381 gcgccaaacc acttttcgac aagctgacaa ccggaatgct cgtgggcccc ctgtgcgcag
    19441 accggggaag cgagcatcaa ctcgcttgcc aggcgcgtgc tgcgctatcc acgtcgacga
    19501 tggcctgctc caatcgatcc agtgacgtcg cgccagcgga gccgctgggc tcgcccgtgc
    19561 cggaagtgcg gtcgcgctga acacggccac cggcgctggc gagcggcaac gtatgagcca
    19621 cggcgcgggc tcggccggcg ctaacggtct ctgctcggcg cgacagcgcg cggagcttgc
    19681 acgagatgtc ctgaatctgc tggttcacgc tggcccgaga ggcgctctcc tgcgaggcga
    19741 ctagcaaagc accgcggagt tcattggtgc gctgccgaac gacgaacacc agcgtcgaca
    19801 gcgagtccac gaactgagcc atacgccggc ccgagacgaa cagacgattc attagcgggc
    19861 gtgcggccag caccaaagag gcaaggtccg cttcgagctt tcgtcccgac tccaagagct
    19921 caatcgcttc ccgctgcgag tcgagcgcca gcttcaaata ggcttccagc tcgagcagca
    19981 gctgtgccgc ctgcgtgcgc acgcgaggca gagccggcgt gggcagcacg agagcagccg
    20041 ccgctacggc cgccgcggcc ccaacgagag tctcgagtac gcgcagctcc agcagaccgg
    20101 cgctgctccg cccgagcagg gaatacagaa ctgcaagcaa tgccgtgatg cagaatacca
    20161 tccctgcgta ggacacccgc agtaggtaaa agcccaaaaa gatgaagaag aatacgcacc
    20221 ccaactcgag ccatctgtgg ccggtcagca catgcgccaa cagcgagcct gccagcacac
    20281 caatcaacgt gccgagtacc cggtgccagg cgcggagcaa cgtgtccgaa accgtctgaa
    20341 cctgggtaaa caccacgaag gcggtaatca ctgcccagta ccagcgatcg ggggacaacc
    20401 ggtacccgag caagatagcg agtgcacaag ccaacgtcgc ttggaaggcc tgacgtgtac
    20461 tgaccgcgat ggacgacggg gtttgggcgg ccgtgttcac ctccacggcc accggctccg
    20521 ctatccgctg gttcccgttc ggctggtttc cgttcggagc tgcgcgcgga acagcactgg
    20581 ggactcgcgg atcacgagca gcgcccaatt cttggactct tgccaacgcg tgcatcagcc
    20641 gatcgaactg cagtttcaac gccgaacaat ccacccctcg gagctcgccg ggcgcaacgc
    20701 gatccaaacc ggggggatgg ctcggttcga gactacgttc gaactgcgcc aaggactgca
    20761 tgagttgctg gcagcgccct gcgccatcct ccttctgccc gagtgaggca cggacctgac
    20821 gcgcgagttg acccgcggcg agctccaaat cgagcgcggc caaccgaatc ggcagccgcc
    20881 ccgagtctcc gccattctgc aagtgttcgt cgagcgtgag cgccacttcg ttcagtctca
    20941 ggagcgcacg gcgcaccccg agacttcggc tcccgcgttc gggtcgcgca cagactcccg
    21001 caagctcgcg aaccaatttg cggatcacga gcggcacaca ggccaaagtg ctgtcgatca
    21061 gctgactcgg ggtttcccgc actacggcga agcgaaccac gaaagcaatc gcgactccga
    21121 tcgcgacact gcctacaatc gccggaatcg ccgatacggg ggctctaaag aactccgcat
    21181 cgaagtaggc gaaggtcgcg atcgtaccga gcgcggtggc tctcgagccg tagcggcgag
    21241 cgtatacact cgcgaacacc acccccaaga acaccgccga ggacagccac agccaccccg
    21301 ccaccagcgt cgagagcgtg gacgcagcca atgcggggaa caccagtaga gcttgagtcc
    21361 gtcgttgctc ggacagcgcg ggctcgcgcg ctgaaatcga gctcatcatc ccaatcgcca
    21421 ccccaagcat gggcgcagcg ctaggccatt ccagagatcg cgccaagcca aacagaacca
    21481 ggacgctcag agcagcggtc aacgccgcac gggcccccat tcgtacccgc gccagatcgg
    21541 catcggcaat ccgcagctcg tccaaccagg caaacgggcg cgagcgagta gacgcttttc
    21601 ctttcgagcg cgcaacaggt gtgtgagcaa cctgaacgcg cgtgtcagcc tcagcgcgat
    21661 tctgtagcat gcggattgta cgggcgccga tttttcgggc cgcgttcacg caatggtgct
    21721 gcaagcacca tgcgcaggcc gccaggcgat ggtgggcttc ggctattctt cggctaatcg
    21781 catcgaactc accaacttcc caggctcagt gaggccatcg cgtaggctcc ccgtaaaccg
    21841 acggtggggc cgtccgaggc cttgccgttg tccgcgtgcc aatcgcgata aggaaagcca
    21901 tagagcccgc ccacgcggac accgagagcc cagccggatt gggccgtatc gaccggggct
    21961 ttgcggctga gtaagatcaa attcgccaag atcgagatcc cgccgaccac gcctttgctc
    22021 gtcaaggtcg tgccacgacc cgggctgcgc agcgcatcgt cgaaccgagt atcgacccgg
    22081 gaccccacgt tcacggtcat accatagccg ccgatcgacg cgatcggcgc caacaagaag
    22141 ccgttctttc ggatcaattg atatccgaaa tcgaagcttc cccaagcgcc actcaccgag
    22201 acgtccgtgt cgccggcccc tctcgtgctt cgcggcaaga agccaactcc cgaaccaccg
    22261 atcaagaagt gactgaatag cccgaagccc tgcccaccga tggtcggaaa cagccccggc
    22321 acgctgttag gatgcccggt tgcttctaaa cgatcgttta gatcccccag atccaaaagc
    22381 agcccgccag ccaagaagct caatccaacc agtttcgggg caccgctgac tggggccggg
    22441 ggcgcctcgg gtttgccttc gagatccggt ttgttcagct gcagtttatc ccctggcgcc
    22501 tgctcctgcg cggaagcgtg acttggaagc gagcacaggg cagagataac gacgcaggaa
    22561 aacgcgctga cgtagcggaa tcgcggcatg tgcatcctct cgatgatgtc tctcgatgtt
    22621 cgagcttgct tggtgtgcgt gagttgcccc gcatgcgggc atacacgacg agcgacacga
    22681 cggtgtctta cacaaagcaa acggagtgcc ggacaggcag gtgttagttc cggccacgat
    22741 tctgcgcgaa tcttcgagga aaaagccggt tcgtccgcct tcgacgtgga aatcacgcgc
    22801 aagcacgcct cacgtttaat agaacctgag gtgcaaatgc gatttgtacg cgaatccacg
    22861 aggtcacgat tggtctgggt gatttgccgc gttaccccat tcgcctcgat gatcgtgcct
    22921 tgcctctgaa tgggcacacc cctccggctc tcgagtaaag ctggtgctgc tcaacacacg
    22981 ttcgatgaag acgtgagctt cctcgcctgc gggactcact ctttcagttc acagggcatc
    23041 gcgggtagag ctctgggccg cgcacgcgcc gaagcgcgca actccgcacc atggtcaact
    23101 cgcttcgtgc gcgtcagttt ctcgcgaggt ccgcgcccga ttcttactaa gaacctatcc
    23161 cggtaagagc gccggagact agctcccatt gggatagttc caatgggtcc gcttctgagc
    23221 gagtcgactt tttcctcgtg gcccacgcct tgctttgtcg agttggcgcc gcgctgtgcg
    23281 ggagtcttat ttcaacaaag ggtttgaaac agcatgtcac cgtcgatggc taggagttct
    23341 ctcgggcgag tgggagctac gattgcgcgg gctttggtgc ccgcgtgttt gtgcacttgt
    23401 gtggcgttgc cggccatggc gaagccgtgg aagggcgcag aactcatcac ccgtcagacc
    23461 ttccgctatg gggcgttcga agccaggatt cgcgccgcgc gcgggtcagg catggtcacg
    23521 ccgttcttcc tctggaagga tggctcggag cgcgccgatc agctctggca agaacaagac
    23581 ttcgagatct tcgggcgcga cggctccttg caaacccagc tcatgacccc agggtccaac
    23641 gaccaacacc gcaccgaaca cgtggtcaac cactggcttc cacaggccgc gttcgagcga
    23701 tactacacct atcgcatgga gtggaccccc acccgcctgg ccttctacat cgacggcaac
    23761 ctcatgc
//
LOCUS       DASTKU010000324         8263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1178524, whole genome shotgun sequence.
ACCESSION   DASTKU010000324 DASTKU010000000
VERSION     DASTKU010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178524"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VFQ61_11560"
     CDS             <1..1182
                     /locus_tag="VFQ61_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9955135.1"
                     /translation="FVIGRGQQQRVFRRKLLDRGRSRALALGWMFVAATSCKGWFESG
                     SVKGKPGSSAGTTVAAPPGLPAGAPVPAGAPSSFADLVARADPAVVFIKTQQEREGFG
                     GRVLGTALGSAFIYDSNGLILTNHHVIEGATDIQVIIGQSRVLSAKVIGKDPPTDVAV
                     LKVEATELPTLPLGDSDAVRVGDWVIAIGNPFGLTHTVSAGILSAKDRTNDQLRESGG
                     GLDTEGGYYDFLQTDASINPGNSGGPLLDSAGRVVGINAAIRDRANSIGFAIPINMVR
                     EILPRLLQDGEIRRSAIGVKVATVLPDYVSKLGLPGPGGALVRAVVAGGPADRAGVAV
                     DDVIVAFDNKPVPAPEKLRWLASLAGVDRTVTLKIVRGGRTLELRVKLNALAAPARTP
                     E"
     gene            1508..2176
                     /locus_tag="VFQ61_11565"
     CDS             1508..2176
                     /locus_tag="VFQ61_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9955136.1"
                     /translation="MNSPDGRASNRVQREADAELDRELIQRAQSGDTAAFRQLVERHQ
                     RRAFAIAVALVKDEEEAREVVQEAFIRVYRGLERFHGGSSFFTWLYRIVTNLAIDVIR
                     KPSRRDTSLEETIAQEDAYPEDSPFSLLEDVDPLDAMHRGQLRANIEAALEELPPYHR
                     SVIVMREVLGMSYEEMAEAMSVSKGTIMSRLFHARRKLQKTLLACYVNAFDEEPELEE
                     TDKP"
     gene            2173..2940
                     /locus_tag="VFQ61_11570"
     CDS             2173..2940
                     /locus_tag="VFQ61_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955137.1"
                     /translation="MKREELIMRHYDGELDAGRVPELERSLASDPRARTELEQLELLG
                     SLVREARAIPDIDFSARIFEKLGLSADGALGTSQGADCSASSEKNSVPESSAGPAPFG
                     APAPIVERSGMGTAAVERAVVERAVVERALVERAAPSAPRHSRRIRPALALAGVSSAL
                     ALAAAVALWLTRPAPSAFQAVAAQVSSPTSDIAAPAPAISVPDPEPPEQSVAVERVDF
                     GSSQGAIFLVPNADERTLVVWTLDELTDDDEGEEADL"
     gene            3005..3586
                     /locus_tag="VFQ61_11575"
     CDS             3005..3586
                     /locus_tag="VFQ61_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955138.1"
                     /translation="MKARYILQLSLPAFCLLAVARKEVSARAPSFAEPPPRTAPAAKS
                     AGKPGAGAQAAPSTSGAAPAPQTHFAAEVLVLHATNEKKGIDPRIAAMPELRKPPFSS
                     YDSYALLERNQFSLDPRHPKTLTLPNGRVLETRLLEILATDSVRLSASINQPGGKEFL
                     PLLEVKAKLGQAFIVAGQRYKRGILVLVIRVSH"
     gene            complement(3681..4928)
                     /locus_tag="VFQ61_11580"
     CDS             complement(3681..4928)
                     /locus_tag="VFQ61_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9955139.1"
                     /translation="MNEDASEFTPSEFAHRRGPNALRSTSTSTETKLPLEALLDLSQE
                     LEPEASVEELVALFTTRLTTLLPGCQIDIRLDPGHFPAASASSAAPRSGYRVVDDSRP
                     DSERPVSDLLDQDTTHVIPLLGLGASCMRVEGSHGDPPSLWEIELCCKAAQVLGRALH
                     RSLAHHCLELRASEADRLRAQLVHSDRLATLGQLVAGVVHELNNPLTAILAYSEHLRG
                     TLERRPVGPEDLQRLQRIQEAAQHILGFSRDLSSYARAQRNEPRRVPLREIVERALAL
                     CEHDLNSSGIRFELNAVADLPALCGVPGQLTQVFVNLFSNAIQAMPNGGLLFVEAALD
                     STGRSLCIHVRDTGVGISAAHLPRVFEPFFTTKPDGQGTGLGLAIVRELVEAHRGCVT
                     VESEPPGGTTFTLRLPLDSEVTA"
     gene            complement(4925..6955)
                     /locus_tag="VFQ61_11585"
     CDS             complement(4925..6955)
                     /locus_tag="VFQ61_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9955140.1"
                     /translation="MASEREILIVDDDNLSRKLLRNALAAAGMNVVALGGGREAIAYL
                     ESRIPRALLLDLVMEPPDGYAVLAHVRQSSRLAELPVVVLTGLDSEEEIQRIFASGAD
                     DYVHKPFRPTELVLRIKSQLRMREAVERLSQRERDQDIVLGLTQQLASTLDVRAILFT
                     VVERVAKLARVDRCSIVLFADSGQLGYVLATSDDPRLRDLPIDLAKYPEIREVLANGR
                     NLVIRDAERHPLLEVVRRDEPARGFHSLALVPIVHDLGPMGVLFLRARAKAELSDSML
                     ALVSTLANATAIALRNARILENLQRQSEQTAVARDDAERRVRLFQRYADFFESAAEAM
                     LVIDRTGRILFANPRSVEVTGLSHDELLSLDLVRLFDPSEQPRTQLLLQGFTRGQYPR
                     DIDLALRRRREVQRSELVTVSASFSSVLHEQDAIFISLRDVTQERHTAVELVRTKEFL
                     ESVIDSSVDGIVSANLRGKVLLFNRAAARIFGYGPDDVVGKLRVDKLYPPGGAREVMQ
                     KILNPDLFGYGRLEDYRVDMLAADGSLIPVQLSASLVLDDGRPIGSVGIFTDIREKLR
                     IQSELERAQEELRLREKQAMLAELAGTTAHELNQPLTSVLGYAALLKRKLADTPEHLP
                     LLDVIIEQADRMAKIVKKVGHITHYETKSYVGTTKILDLDKASGLDPEGLPE"
     gene            7432..8232
                     /locus_tag="VFQ61_11590"
     CDS             7432..8232
                     /locus_tag="VFQ61_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955141.1"
                     /translation="MAKRKELGVDCRTVRNPTERVVGVAAQELGLEPTRGGPMQPQEQ
                     ASTSLPQATALPQPMALPHAKVLPQAGSSPGASAEPLEELGDDAIIAQQTVAELPKRE
                     QINEEARSVVISDLPPPRSGQRAQRGRGDPTIVIRDRRELDAARQRFLEEHRRSQEQE
                     KAHQAAQKSRSMYYVMGLGLLAFAIGGGVALAKSRHQRAVTQQRIATQRELQASRAAA
                     ARRATAVIAPRAPGQANPASGASAAEASDSPAAEEREAAPNRVPRGQP"
BASE COUNT         1556 a         2553 c         2692 g         1462 t
ORIGIN      
        1 ttcgtgatcg ggcgcggaca acagcagcga gtgtttcgcc gaaagctgct ggatcgcggg
       61 cgaagtcgag cgctggcgct ggggtggatg ttcgtcgccg cgaccagctg caagggctgg
      121 ttcgaatccg gaagcgtaaa aggtaagcct ggaagcagcg ccggtacaac ggtagcggcg
      181 ccgccagggc tgccggcggg tgcacccgtt ccagcgggtg caccgagtag cttcgctgac
      241 ttggttgcac gcgcggatcc ggcggtcgtc ttcatcaaga ctcagcaaga gcgcgagggg
      301 ttcggagggc gcgtgctcgg cactgcgctc ggaagtgcct tcatctacga ttccaacggt
      361 ctgatcctca cgaatcatca cgtaatcgaa ggcgcgaccg atattcaggt catcatcggt
      421 cagtcgcgcg tgttgagcgc gaaagtgatc gggaaagatc cgcccacgga cgtagccgta
      481 ctcaaggtgg aggcgacgga gctgcccacg ttgccgctcg gtgactctga cgccgtgcgc
      541 gtaggcgact gggtcatcgc gatcggtaac ccgttcggat tgacgcacac ggtctcggcc
      601 gggatcctct cggcgaagga ccgtacgaat gaccagctcc gcgagagtgg gggcggcttg
      661 gacaccgagg gcggctacta cgattttctc cagactgacg cgagcatcaa ccccggcaac
      721 tctggtgggc ccttgctgga tagcgcggga cgggtggttg gaatcaacgc cgctattcgc
      781 gatcgcgcga atagcatcgg gttcgcgatt cccatcaaca tggtgcgcga gatcctgccg
      841 cggttattgc aagacggtga aatccgccgc agcgcgattg gcgtgaaggt cgcgacggtg
      901 ctcccggact atgtctcgaa gttgggctta ccgggaccgg gtggcgcctt ggtgcgggcc
      961 gtggtcgcgg gagggcccgc ggacagagcg ggcgtcgccg tcgacgacgt tatcgtggcg
     1021 ttcgacaaca aaccagtgcc cgcaccagaa aagctgcgct ggctggcgag cttggccgga
     1081 gtggatcgca cggtgaccct gaaaatagtg cgaggcgggc gaactctgga gctgcgagta
     1141 aagctaaacg ccctcgctgc cccggcccgc acgcccgagt agaggcctgg ctgcgcaaag
     1201 ggccgagtaa acgggcactc gtcgaagctc gtgcctgcgc cgaactggct gagtaaaagc
     1261 agcgcgcggg gcaaacccaa ggcgcacccc ctcgttcaat gctagcagac tcacttccga
     1321 gcccaggttt ctgaggaatg tctcgcagga gcatgcgtac gttctatgtg cgatggcgct
     1381 ccacgcagag ctgtcggccg gtgcggcgag cttgcccacg gcctcgccgg gtatccaggc
     1441 ccagccgccc tccttcggac gagtccgaac aattcgaccg aatctgtcga atcagggggg
     1501 cgtgaccgtg aatagccccg acggccgagc gtcaaaccgg gtgcagcgcg aagctgacgc
     1561 ggaactcgat cgtgaactca tccaacgtgc gcaatctggt gacactgctg cctttaggca
     1621 gctggtcgaa cggcaccagc gtcgggcttt cgcaatcgcc gtggcgctcg tcaaagacga
     1681 ggaagaggcc cgggaggtcg tgcaagaggc ctttattcga gtctatcgcg ggctcgaaag
     1741 gtttcacggc ggctccagct tctttacttg gctgtatcgg atcgtcacca atcttgcgat
     1801 cgacgtcatc cgcaagccct cccggcgtga cacttcactc gaagagacca ttgcgcagga
     1861 agatgcctac cccgaggaca gccctttctc gctgctggag gacgtcgatc cactcgacgc
     1921 aatgcatcgg gggcagcttc gagcaaacat cgaagcggca ctcgaagagc tcccacccta
     1981 ccaccgaagc gtcatcgtca tgcgcgaggt gctcggcatg agctacgaag agatggccga
     2041 ggcgatgtcg gtgtccaaag gcacgatcat gagccggctc tttcacgcgc ggcgcaagct
     2101 gcaaaaaacg ttgctggctt gctatgtgaa cgcattcgac gaagaacccg agctagaaga
     2161 gactgataag ccatgaagcg ggaagagctc atcatgcgcc actacgatgg cgagctcgat
     2221 gcggggcgag tgcccgaact cgagcgctcg ctcgcgagtg acccgcgcgc tcgtaccgaa
     2281 ctcgagcagc tcgagctgtt gggaagcctg gtgcgagaag ctagagcgat tcctgacatc
     2341 gacttttcgg cacggatctt cgaaaaactc gggctctcgg cggatggcgc ccttggcact
     2401 tcccaaggag cggattgctc cgcatcgtct gagaagaatt cggtgcccga gagttcggcg
     2461 gggcccgctc cgtttggcgc gccggcgcct atcgtcgaac gttccgggat gggaactgcc
     2521 gccgtggaac gcgccgtggt ggaacgcgcc gtggtggaac gcgccctcgt cgaacgggca
     2581 gctccctcgg cgcctcggca ttctcgcagg atccgccctg cgcttgcgct cgcgggcgtg
     2641 agctcggcgc tggcgttggc cgcggcggtt gccctatggt tgactcgtcc ggcgccctct
     2701 gcgtttcagg ccgtagcggc ccaagttagc tctccgacgt cggatatcgc cgcgccggcg
     2761 cccgcgatct cggttccgga tcccgaaccg ccggaacagt ccgtggccgt cgagcgagtg
     2821 gacttcggct cgagccaagg tgccattttc ttggtgccga acgcggacga gcgcactcta
     2881 gtcgtgtgga ctctcgacga gctgaccgac gatgatgagg gcgaagaggc tgatctgtga
     2941 ttctgcctga atccagcatc ggctcgagca tgctttactc gctggggaag attcgttcgc
     3001 gcgcatgaaa gctcgttata tccttcagtt gtcgctgccc gccttctgtc tgctcgcagt
     3061 ggctcgcaaa gaagtcagcg cacgcgcccc gtccttcgcc gagccgccgc cgcgaaccgc
     3121 tcccgccgcg aagagcgccg gcaagccggg tgcgggcgcg caagcagctc cgtctacgtc
     3181 gggcgccgca cctgcaccac agacgcactt cgccgccgag gtactcgtgc tgcacgcgac
     3241 caacgaaaag aagggaatcg acccgcggat cgccgcgatg cccgagctgc gcaagccgcc
     3301 cttttcttcg tacgacagct atgcccttct cgagcgaaat cagttctcgc tcgacccgcg
     3361 gcatccgaag acgctgacgc tccccaacgg gcgtgtgctg gaaacgcggt tgctagagat
     3421 tttggctacc gacagcgtcc gactttccgc cagtatcaat caaccgggtg gaaaggagtt
     3481 cctgccgctg ctcgaagtga aggccaagct agggcaggca ttcatcgtgg caggccaacg
     3541 ctacaagcgc ggaattttgg tgctcgtgat tcgtgtcagt cactgagtcg agccgcggca
     3601 cagcccggga gcgacagagt tagcgaacga cagagttagt gccgaagtcg cccatcgcgt
     3661 cgcgtcggcc gcaagccgga ctaggccgta acctcactgt ccagcggcaa gcgcaacgtg
     3721 aacgtggtgc cgcctggggg ttcactttcg accgtgacgc aacctcgatg cgcttccacg
     3781 agttcacgca cgatggccaa ccctaggccg gtgccttgcc cgtcgggctt tgtcgtgaag
     3841 aacggctcga aaacacgcgg tagatgggca gcgctgatgc ctacccccgt gtcccggacg
     3901 tgaatgcaga ggctgcgccc ggtgctgtcc agcgcagctt ccacgaacag cagccctccg
     3961 ttcggcatcg cctgaatcgc attcgagaac aaattcacga acacttgcgt cagctgacca
     4021 ggcacgccgc agagcgccgg caggtcggcc accgcgttga gctcgaagcg gatgccgctc
     4081 gaattcaagt cgtgttcgca tagcgcgagc gcgcgctcga caatctcgcg cagcggaacc
     4141 cgccttggtt cgttgcgttg cgcgcgagcg tagctcgata gatcccgcga gaaacccagg
     4201 atgtgctgcg ccgcctcctg aatccgctgc aagcgttgca gatcttcggg ccccactggt
     4261 cgccgttcga gcgtaccccg aaggtgctcc gagtaggcca aaatggcggt gagcgggtta
     4321 ttgagttcgt ggaccacgcc ggccacgagt tggcccagag tcgcgagacg atcgctgtgg
     4381 acgagctgcg cgcgtaaacg atcggcttcg gacgcgcgca gctcgaggca gtggtgggcc
     4441 aagctgcgat gcagcgcgcg ccccaacacc tgcgccgctt tgcagcataa ctcgatctcc
     4501 cacaggctcg ggggatcgcc gtgactgcct tccacccgca tgcacgaggc tccaagtccg
     4561 agcagcggga tgacgtgcgt agtgtcctga tcgagcaagt ctgaaacggg tcgctccgag
     4621 tccgggcgcg aatcgtccac gactcggtag ccagagcggg gcgcggcgct gctggccgac
     4681 gcggcgggga agtgaccggg atcgagcctg atgtcgattt gacatcccgg gagcaaggtc
     4741 gtgagccgag tggtgaagag cgcgaccagt tcctctacgg aagcttccgg ttcgagctcc
     4801 tgactgaggt cgagcaacgc ttcgagcggc agcttcgtct cggtcgaggt cgaggtggag
     4861 cgaagcgcgt tagggccgcg ccgatgcgcg aactcactgg gcgtgaactc cgaggcgtcc
     4921 tcgttcattc gggcaagccc tccggatcca atccgctcgc tttgtcgaga tccaaaatct
     4981 tggtcgttcc gacgtagctc ttggtctcgt agtgagtgat gtgccccacc ttcttgacga
     5041 tctttgccat tctgtccgct tgctcgatga tcacatccag caaaggtaag tgctcgggcg
     5101 tatcggcgag cttgcgcttg agcaaggcgg cgtagccgag aacgctggtg agaggttggt
     5161 tgagctcatg agcggtggtg cccgcgagct ccgcgagcat ggcctgtttc tcgcgtaaac
     5221 gaagctcttc ctgcgcgcgc tcgagttccg actgaatccg caacttctcg cgaatgtcag
     5281 tgaatattcc gaccgatccg atcggcctgc cgtcgtccag caccaaggac gccgagagtt
     5341 gcaccggaat caaagagccg tcggcggcca gcatatcgac gcgatagtct tcgagccgcc
     5401 cgtaaccgaa taggtccggg ttcaagatct tctgcatgac ttcgcgggct ccaccgggtg
     5461 gatacagctt gtcgacgcgc aacttgccga cgacatcgtc tgggccgtag ccgaaaatac
     5521 gggcggcggc tcgattgaac aacaagacct tgccgcggag gttggccgag acgatgccat
     5581 cgacggagct atcgatcacg gactccagga actccttggt gcgcacgagc tcgacggccg
     5641 tgtgccgctc ttgagtgacg tcgcgcaggc tgatgaagat tgcatcttgt tcgtggagaa
     5701 ccgagctgaa actggcgctc acggtcacga gctcactgcg ctgaacctcg cgccgccgac
     5761 gcaacgccag atcgatgtcc cgcgggtatt gcccgcgcgt gaagccttgg agcagtagct
     5821 gggttcgggg ttgttcggag gggtcgaata gccgcaccaa atccagggac agcagctcat
     5881 cgtggctgag gcccgtgact tcgaccgagc gtgggttggc gaacaagatg cggcccgtgc
     5941 ggtcgattac cagcatggcc tccgcggcac tctcgaagaa atcggcatat cgctggaaca
     6001 accgtacgcg ccgctcggcg tcgtcgcgag ccacggcggt ctgctcgctt tgccgttgca
     6061 ggttctccaa gatgcgcgca ttgcgcagcg cgatcgcggt ggcgttggcc agagtgctga
     6121 cgagcgcgag catggagtcc gagagctcgg ccttggcacg cgcacgcagg aacagcaccc
     6181 ccatgggccc caagtcatgg acgatgggca cgagcgcgag tgagtggaaa ccgcgagcgg
     6241 gctcgtcccg ccgcacgacc tcgagcagtg gatgtcgttc ggcatcccga atcacgaggt
     6301 tgcgaccatt ggccaatacc tcgcgaattt ctggatattt ggccaaatcg atcggcaagt
     6361 cacggagccg ggggtcatcg ctagtcgcca gcacatagcc gagctggccc gagtccgcga
     6421 acagcacgat gctgcagcgg tcgactcgtg ccagcttcgc cactcgctcc acgacggtga
     6481 aaaggatggc gcgcacatcg agcgtggagg cgagttgctg ggtaagaccg agcacgatgt
     6541 cttgatcacg ttcgcgttgc gaaagccgct cgacggcttc gcgcattcgc agctggctct
     6601 tgatgcggag cacgagctcg gtgggacgga agggcttgtg aacgtagtcg tcagcgcccg
     6661 acgcgaaaat gcgttggatc tcttcttcgg aatcgagacc agtcagcacc actacgggca
     6721 gctccgcgag gcggctggat tggcgcacat gggccagcac cgcgtagcca tccgggggct
     6781 ccatcacgag atccaacagc agcgcgcgtg ggattcgtga ctcgaggtat gcaatggctt
     6841 ctcggccgcc gccgagtgcg accacgttca tacccgccgc cgctagcgcg ttgcgtagca
     6901 gttttcgact caaattgtcg tcgtcgacga ttaggatttc gcgctcggag gccacgcaga
     6961 gctctcgacc aagatcgttt ggagaacttt cgcgcgctca cgctaccatg gcaacacggg
     7021 ttcgagcagc ttgggttgaa aggggcgtgg gggcggggcc cggagctccg ccagccgaac
     7081 ttgcgctcaa tccagcagcg ctgcggccta tgggtccggt ttcactcatc gcatctcagt
     7141 cgacgatttc tgcggctaag cgagctttcc ggcgcttggt tcgccggatt tatgagccga
     7201 atttgcgccg tgcccgggcg ggcgcttctg tgttgcgcgc agatttggcc tgtttagcgg
     7261 gcgctactgg cggcggcccc actactctga tcggctcgag ggctgcgaga aggcgggttc
     7321 gctaccgaaa caggcatgaa atcccccgtc cgtgcccact aagagttggc tcggagagcc
     7381 cgagagcaag acaaattcct atgggcgaac gaaatatcca ggcgcagagc aatggctaaa
     7441 cgcaaggaac ttggggtaga ctgccggacc gtgcggaatc ccaccgagcg tgtagtcggc
     7501 gttgccgccc aagaacttgg gctcgagccc acccgtggag gccccatgca gccccaggag
     7561 caggcgtcca cgtcgttgcc gcaggccacg gcgttgccgc aacccatggc gttgccgcac
     7621 gccaaggtgc tgccgcaagc tgggagctct cccggcgcat ccgcggagcc gctcgaggag
     7681 ctcggcgacg atgcgatcat cgctcagcag accgtagctg aactcccaaa gcgcgagcag
     7741 atcaacgagg aggcgcgatc ggtcgtcatc tccgatctgc ccccgccccg ctcaggccaa
     7801 cgcgcgcagc ggggccgtgg cgatccgacg atcgtgatcc gggatcgccg agagttggac
     7861 gccgcacgac agcggttcct cgaggagcac cggcgtagcc aagagcagga gaaggcgcat
     7921 caagccgccc agaagagccg ctcgatgtac tacgtaatgg gactcgggct gcttgcgttt
     7981 gcgattggcg gtggagtggc gctcgccaag tctcgccacc agcgcgcggt aactcagcaa
     8041 cggatcgcga cgcaacgaga gctgcaagcg tcgcgggcgg ctgcagcccg gcgagccaca
     8101 gcggtaatcg cgccacgagc accgggccag gccaatcccg catcaggtgc cagtgctgcc
     8161 gaggcttccg attccccggc tgcagaagag cgagaagccg cgcccaatcg cgtgccgcgc
     8221 ggccagccgt agcgcaccac ctgcagagca aagagctctg tct
//
LOCUS       DASTKU010000325         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1625217, whole genome shotgun sequence.
ACCESSION   DASTKU010000325 DASTKU010000000
VERSION     DASTKU010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625217"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="VFQ61_11595"
     CDS             <1..1287
                     /locus_tag="VFQ61_11595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9955142.1"
                     /translation="LTGVFPVHDATGAITLSGAVLIEISDRKRLEAQRAALLDSERAA
                     RIEAERAATLKDQFLATLSHELRTPLNAILGWARIARQSAARPPELERPLEVIERNAR
                     AQAQLIEDLLDISRIVSGKMRLTKEAIELTQVAQAAVTAAAPAAEAKQIQLGFEAQPA
                     LPPIDGDPTRLQQVVSNLLSNAIKFTQRGGSIQVTLTRRGSNLELRVTDDGAGISPEF
                     LPHVFDRFRQADSSTTRRHGGLGLGLSIVRQLVELHGGTVLAESPGPQKGASFIVSLP
                     IVVDADRARDSGMLRWLLPEAALSGVRVLIVDDEPDAREFVGRILMDLGAQTRLAASV
                     DEALDCLHPEPAHIIVSDISMPGRDGYELIHAVRQLYDGKTVPAIALTAFARSEDRMR
                     ALSAGFQLHVAKPVDPHELTLAVASLVGRTGAAPAA"
     gene            1397..1900
                     /locus_tag="VFQ61_11600"
     CDS             1397..1900
                     /locus_tag="VFQ61_11600"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET9955143.1"
                     /translation="MGSSSPVTPAGFAGYAGMQPFSSSIGSRNEIGSKLRAHFALPAV
                     ALACLGALAACNRNDNKTTAQSESTAAQQPAIVTHDSSRPLELTVTSDGFVPAQVKVE
                     AGKPLTLWVTRKADPTCATEIVIKDYGINKPLPLNERVEVVFTPKPGKIRYACAMDMI
                     AGELIAQ"
     gene            1911..>2359
                     /locus_tag="VFQ61_11605"
     CDS             1911..>2359
                     /locus_tag="VFQ61_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955144.1"
                     /translation="MQVARCKMARVEVFALALACALGLSARASADPANAASQSPPGGS
                     SRGAVASPTVLPSDATLDQLIRDSIAARPELARAKATLRASQERVPQASTLPDPMVQV
                     GIQNDGFKSIEVGRMETSYISLMASQTFPWPGKRDLRGRIAALDIGEA"
BASE COUNT          437 a          781 c          740 g          401 t
ORIGIN      
        1 ctgacgggtg tgttcccggt ccacgacgcc accggcgcga tcacgctctc cggcgcggtc
       61 ttgatcgaga ttagcgaccg caaacgactg gaggcgcagc gcgcggcctt gctggacagc
      121 gagcgcgcgg cgcgcatcga ggcggagcga gccgccacgc tgaaagacca gttcttggcc
      181 actctatccc acgaactccg cacaccgctg aatgccattc tgggttgggc gcgtatcgcg
      241 cgacagagcg ccgcccgccc ccccgagctg gagcgacctt tggaggtgat cgaacgcaac
      301 gcccgagcgc aggcccagct gatcgaggac ctgttggaca tttcccgaat cgtcagcggc
      361 aaaatgcgac tcaccaagga agccatcgag ctaacacagg tggcacaggc cgccgtaacc
      421 gcagccgcgc ccgcggccga agccaagcag atccagctcg ggttcgaggc tcaacccgca
      481 ttgcccccca tcgatggaga tccaacgcgc ctgcagcaag tcgtgtccaa cttactctcc
      541 aacgccatca agttcaccca gcggggcggc agcatccaag tgacgctgac tcggcgcggg
      601 tccaacttgg agctgcgcgt caccgacgac ggagccggaa tttctcccga gtttctcccg
      661 cacgtgttcg atcgatttcg ccaagcggac tcgagtacca cccggcgcca cggcggcttg
      721 ggcctggggc tatccatcgt caggcagctc gtggaactcc acggaggcac cgtgctcgcc
      781 gaaagcccgg gcccccaaaa aggagcgagc ttcatcgtct cgctgcccat cgtcgtagac
      841 gccgatcgcg ctagagattc tgggatgctg cgttggctat tgcccgaggc tgcgctgtcg
      901 ggagtgcggg tgttgatcgt ggatgacgag cccgacgcac gcgagttcgt cggacggatt
      961 ttgatggatc tcggcgctca gactcgcttg gccgcgagcg tcgacgaagc gctggattgc
     1021 ctgcaccccg agcccgctca catcatcgtg agtgacatca gcatgcccgg tcgggatggc
     1081 tacgaattaa tccacgcggt ccgtcaactc tacgacggga agaccgtccc cgcgattgcg
     1141 ctaacggctt tcgctcgctc cgaagaccgg atgcgcgcgt tgagcgccgg gtttcagctg
     1201 catgtggcca aaccggtgga tccgcacgag ctcacgctcg ccgtggccag tttagtgggg
     1261 cgaaccggcg cggcgcctgc cgcttgacgt ttgtcaggta cgcccagctc agctccgttc
     1321 gtcttggcga ggtgcgggtc ggggggccgc gtcccgcgag aatgggtgag tcagggggag
     1381 tccggcaatt cgcgatgtgg gcagctcgtc acctgtcacg ccggcaggct ttgctggcta
     1441 tgcgggtatg caacccttct cctcctcgat tggcagcaga aatgagatcg gcagcaaact
     1501 ccgggctcac ttcgcccttc cggccgttgc cttggcctgc ctcggtgcgc tcgccgcctg
     1561 taatcgtaac gacaacaaga ccacggccca atccgaatcg accgcggccc aacagccggc
     1621 aattgttacc cacgactcct cgcgtccgtt agaactcacc gtaactagcg acgggttcgt
     1681 gcccgctcag gtcaaggtgg aagccggcaa gccgctgacc ctctgggtca cacgcaaagc
     1741 cgacccgacc tgtgccacgg aaatcgtcat caaagactac ggcatcaaca agccgcttcc
     1801 tttgaacgag cgagtcgaag tcgtgttcac gcccaagccg ggcaagattc gatatgcgtg
     1861 cgccatggac atgatagccg gcgaactcat cgctcagtag tgtgcgtccc atgcaagtcg
     1921 cgagatgcaa gatggcgcgc gtggaggtgt tcgcgctggc cctggcgtgc gccctcgggc
     1981 tgtcggcgcg ggccagtgcg gacccggcca atgctgcgag tcagagtccg cctggaggtt
     2041 cgagtcgcgg cgcggttgcg agcccgaccg tactgccctc ggacgcgacg ctcgaccagc
     2101 tgatccgcga cagtatcgca gcgcggcccg agttggcccg cgccaaagca accctccgcg
     2161 cgagccagga gcgcgtaccc caagccagca cactgccgga tcccatggtg caggtgggca
     2221 tccaaaacga tggcttcaag agcatcgaag tcggccgcat ggagaccagt tatatctccc
     2281 tgatggcctc gcaaaccttc ccttggccgg gtaagcggga ccttcgaggg cgaatcgcgg
     2341 cgctcgacat cggggaagc
//
LOCUS       DASTKU010000326         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_510083, whole genome shotgun sequence.
ACCESSION   DASTKU010000326 DASTKU010000000
VERSION     DASTKU010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510083"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFQ61_11610"
     CDS             complement(<1..896)
                     /locus_tag="VFQ61_11610"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET9955145.1"
                     /translation="MREIQRQRPDTPATVSELPGPFPALGPHSLVVPRAVAESSSLET
                     TQSASESLVEALWALGVRRAYAVFGGGIAPFCEALSRSPIQLLHFRHETGAAFAAVED
                     SLASGQLTVVVATTGPGITNLYTGMAAARSEGARVLFVSGVTSAAQRGRGAFQETSAS
                     STLAPLFDKGSVFHYAGVLEDPAELAPTVSRLASGMTRENGFVAHLGLPMSLQTARAS
                     RTSRPAPTTVERGGVLPEAVERCVEILGREPFVIWAGFGARHAARGVRQLAERTGAHV
                     FASPRAKGVMPENHPLYLGVSGL"
     gene            1198..2289
                     /locus_tag="VFQ61_11615"
     CDS             1198..2289
                     /locus_tag="VFQ61_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET9955146.1"
                     /translation="MLRIEKLGAPPWPTLDPFLFCVHHRDAYPAGNGELGPVADLSSR
                     QLGNDFSGREGWSMYHGRVVPGFPRHPHRGFETITVVRRGLIDHSDSLGAAARYGQGD
                     VQWLTAGQGIVHSEMFPLLDANAPNPLELFQIWLNLPSSSKFVEPRFSMLWSDTIPKR
                     AYRDAQKRQTLVTAVAGQIRDLIPPAPPPNSWAARPAGQIAIWTLKLDSNASFELPAA
                     DATLRVPNRILYFFAGATLRLSGYDSQSAARARSSVTGRSASREIERGHLAELRATQP
                     VLIENGAADAEILVLQGAPIGEPVVKYGPFVMTTEQEIRQAYADYRRTEFGGWPWPSP
                     GPVHGASDSGRFARHADGRLERANRSSSG"
BASE COUNT          546 a          991 c         1023 g          606 t
ORIGIN      
        1 aggccgctga cccccaggta cagcgggtga ttctcgggca ttacgccttt ggctcgggga
       61 gaggcaaaca cgtgagcacc ggtacgctct gccagctgac gtacgccgcg cgccgcgtgt
      121 cgcgcgccga aaccagccca gatcacgaac ggctctcgcc ccaaaatctc tacacagcgc
      181 tctaccgcct ccggtaaaac cccacctcgc tcgacggtgg ttggagccgg tctcgaggtt
      241 cgcgacgctc gtgccgtttg tagactcatg ggcaatccga ggtgcgcgac gaagccgttc
      301 tcgcgcgtca tgccgctggc caaacgcgag acggtgggcg cgagctcagc gggatcttcg
      361 agtactcccg cgtaatggaa cacggagccc ttgtcgaaca agggggcgag cgtagagcta
      421 gcgctcgtct cttggaacgc gccccgcccc cgctgcgccg cggaggtgac ccccgatacg
      481 aacagcacac gcgcgccctc gctgcgggcc gcggccatgc cggtgtatag gttagtaatg
      541 cccgggcccg tggtcgctac cactacggtt agctgcccgc tcgcaagtga gtcttcgaca
      601 gcggcgaaag cggcgcccgt ctcgtgccgg aagtgcagga gttgaatcgg gctgcgcgag
      661 agagcttcac aaaagggggc aatccctccc ccgaagacgg cgtaggcgcg acgcactccc
      721 agcgcccaga gtgcctccac caggctctcc gacgcgctct gcgtcgtttc gagtgacgat
      781 gactcggcaa cagcgcgagg cacgaccagg ctatgcgggc cgagcgcggg gaatggaccg
      841 ggtagttcgg acaccgttgc tggcgtgtcg ggtctctgac gctggatttc tctcatcggg
      901 cgtccctcat ggtcgatgta accgctccag atgtggccgg gctcgatgtg gactgagtca
      961 ggcgctacgc agctgaccaa cgaccttgtc ggctgttgtc cgtgctgaac gccgagcgtg
     1021 cgggcaagtt aatagaggtc tcaagaaaat ttgtcgaatc cacgccgcgc ttgccaacct
     1081 gaatggccgt cctattcctt cgggatgcgc cgtcgtcatc tactgcaagc catcgcgggc
     1141 acaccggtct tggctgcgtg cgagcggtca cggaagttgg agccgcgacc agacccagtg
     1201 cttcgaattg agaaactcgg cgcgcctccg tggccgaccc tggatccctt tcttttctgt
     1261 gttcaccatc gagacgccta tccggcgggt aatggggagc tcgggcccgt tgcggacctc
     1321 agctcgcgcc agcttggcaa cgacttttcg ggtcgcgaag gctggagcat gtatcacggg
     1381 cgagtcgtac cgggctttcc gcgacatccc caccgcggct tcgagacgat cacggtcgtt
     1441 cggcgaggtc tcatcgatca ttccgattca ctcggggccg ccgcgcggta cgggcaaggg
     1501 gacgtgcaat ggctcacggc cggtcagggc atcgtgcact ccgaaatgtt tcccctgctc
     1561 gacgccaacg ctccgaaccc gctcgaactg tttcagattt ggctgaatct tccgagctcc
     1621 agcaagttcg tcgaacctcg cttcagcatg ttgtggagcg atacgatacc caagcgcgct
     1681 tatcgtgacg cgcagaagcg gcagacgctg gttacggcgg tggcggggca aatccgcgat
     1741 ttgattcccc ccgcgccacc gccaaactcc tgggcagcgc gaccggcagg gcagattgcg
     1801 atatggactc tcaaactcga tagcaacgca agcttcgagt tgccggcggc ggacgcgacc
     1861 ctacgagttc cgaatcgaat tctctacttc ttcgcaggcg cgaccttacg actgagtggc
     1921 tacgactcgc agagtgcggc gcgcgcgcgt tcgtcggtca cagggcggtc cgcttcgcgc
     1981 gaaatcgagc gaggccactt ggcggagctg cgagcgacgc agccggtgtt gatagagaac
     2041 ggcgccgccg acgctgagat tctcgtgctg cagggcgctc caatcggaga gcccgtggtg
     2101 aagtacggcc ccttcgtcat gaccaccgaa caggagatcc ggcaagccta cgccgactat
     2161 cgcaggaccg agtttggcgg ctggccgtgg ccgagtccgg gcccggtaca cggggcttcg
     2221 gacagcgggc gattcgcgcg ccacgcggac ggccgcttgg agagagccaa ccgatcttcg
     2281 tcaggttgag tacgcgggtg cgcccccggt cttaggcgtc accgcacgcc cgcccgcgcc
     2341 gagtcacgtg ggcgccttct tcgtgaatgg ggaagaggag aagcccctca attgcgattc
     2401 tcggtcgctg ggggccgccc cgagccgggt gtacccgcgc gggcgagcgc gactttggcc
     2461 cctagttgca gcctccatcg ctcttggttg gatgccctgt gagccgaggt tcgcgtgttg
     2521 aagtcggaat attccactct cgataagcct cgactttcga agctcgcatc acgatgagac
     2581 gctcgggccg ccgaagtctg ggtccttcag agggtgcgat ggctccacgc aatccattgc
     2641 tggaactggt tccggtcagc ttcgaggatc gcgagcgctt gcggcgacga ttttctggta
     2701 gcggttccag ggctgagttc tgcgtacctg cgccctgtgg gctccactcc gcgccgcgca
     2761 atttccctga ttttaggctc tgttcctgta tgggtggcgg cgatgcggtc aattgtcgac
     2821 aggtttgccc gggatagagc tccgcgaaat cgcggcattt cgggagcggc gcgcctgatc
     2881 attgccagtg acgtgcttga ctgactgagt ggagggggtt aatgaaggtg ccgagttcgc
     2941 aacccattgg tgttgcgtat ttacgaattc cgtgcagacc cgagccgagg cgctccgatc
     3001 agggctcctg catggtgctt atcgcctacg gcggcttacg aaagtcgtat tggggcccag
     3061 tattcgccgg gtcgtgagga ttgccgagcc cctgagattt tgcactgtcc cagccgattc
     3121 gctggcggga ggcggagctg gcgcactcac cagctcggct aaccca
//
LOCUS       DASTKU010000327         6059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_63959,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000327 DASTKU010000000
VERSION     DASTKU010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63959"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFQ61_11620"
     CDS             complement(<1..319)
                     /locus_tag="VFQ61_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955147.1"
                     /translation="MLRENCDARKPTPSQALQSPQLCRVARIAANCGLFVAQFLIACS
                     GSQSDPGVKSAGGAAGETIGGMSNLGTAQGNGGASSVMGGGAGLSTAGSGPNTVHVGA
                     GGAS"
     gene            complement(499..2988)
                     /locus_tag="VFQ61_11625"
     CDS             complement(499..2988)
                     /locus_tag="VFQ61_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9955148.1"
                     /translation="MPAINSSPGGRQRTRVALLMRNLGYSYQDSLLQGVHESCQELGY
                     DTICLIGGVLCASDPRNSAYSVIGEGDVDAVIFASGTLGGGEDSPQVQATFARFAGLP
                     RCNISYAQAGVPSICVDNASGVYASTRHLIEKHGRRRLAFLSGPGEESRQRRNGFERA
                     HLDCGLAVDPSWILEGDFSCESGMAAVARLGTPDAPAIDALVAANDWMAMGVLEEAER
                     RGFAIPEQIALIGFDDSDQARFTTPALTTVRQPIAELGRAAVRLITAQLAGEGVPELG
                     YLQTELVVRHSCGCLGHALSPDPTTAKTQAHLLELLGSHRASCQAALVNSMPGLDAVA
                     EFQDQLGWSGELIDALLQDLGSDGGAFVGVLERLLHYSARLGNAKSWHGALSALRGAA
                     IRSGATDISEWFRFESLIERAHIVIGSHAERLQGRRRIENDVQMHALEALGNTVRTAL
                     DYDALSRGLAPHLGSLGLERCWVTLTGEKVDARSVSRLVVPCNEQGDTARGPLEFTTG
                     LVLPTQQMLARPTTYVVLPMIFGNETLGFCILPHGGGEPGSYHQICAPIAATVKAVSL
                     LRTVVTEVTRREAVERERLAQEMAIAQRIQTAIFPKDLRVPGLEVSAAMIPATEVGGD
                     YFDVLPTAEGCWLGIGDVVGHGLPTGLVALMIQSIVAAAAQRGATASPVELWCDVNSV
                     LFDNVRTRLGQNEHATLTLMRYWTDGRVLMAGAHEDLILLRTCAESSEMIQTPGVWAG
                     ITKELSPEAMIQYELQLDPGDLLLLYSDGVTEAMNQDLQMFGVERLRRVFEAHRQKSA
                     YAIREAIMSEVSNWTRSRDDDVTVAVLRYRG"
     gene            3454..5352
                     /locus_tag="VFQ61_11630"
     CDS             3454..5352
                     /locus_tag="VFQ61_11630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9955149.1"
                     /translation="MYKRLPGSGTSTKKLQQLAGRAPSRLAGWGKRSIGYNLSSGAKV
                     WTCCRGVPLRALALSFLCTLVLLIAHAAFELGGAGSAALSLSFVPGLLALLALGPALG
                     WAVSGLMLASMAWLFVVRPPLLRYDLLRFIDQSAMVVFAAGLAHALHRSFGWYEMAIA
                     RRQAVLLDMQEKRTNIAAAIFEQLDPLSSQLVATLSPPTPDRQAIRTLFSRLIEHLRR
                     AKELARTDETDSPDLQEPDRDQLIRCRTMRVWLRLGAFLMAFFVIRNLVAGTPFLPSV
                     FSLAFCLFFDIWLGSSQCSKYLESTALAIGLSASGPMIAHIHAYGGRADAPPLVVTPG
                     IVLFTALLSQGPATWAVVALNVAVLAWVGLGSETDATGSSRLTDLALSFLVIVVAIRS
                     VFALRRRYALALLEQAQALARALRQHRRLAGTLFHDVSNHLQALSLHLEVESTRADLD
                     HALSLSRRVQRLIRLSKEFLLNPQEGPSPALGAVTLDEAVKLLLEAFAPRLGAKQAQL
                     LAGPGLDLRVRAQPELLIESVLGNLLSNAIKFSPLGAQITLKAERIGRSVRIVICDSG
                     RGIPPEVVKRLGQDCVVPSALGTAGERGQGYGLQLVHEHLQRMGGRLELSNRREGGTE
                     AAVWLETA"
     gene            5405..>6059
                     /locus_tag="VFQ61_11635"
     CDS             5405..>6059
                     /locus_tag="VFQ61_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955150.1"
                     /translation="MSLRLRSGRLLGCLVSALFVACQSPVLRAPAAPAGAEGSVLPLA
                     RVRLFENGIGYFERSGTLTATAALLPVPASHVDDALKTLVVLSNSSMTVSGIEFASLV
                     SGGTARALAGLPLSDETPLGYGDVLSSLEGYRVRLTTKSGAQFSGRLIDVQRFERAAA
                     TCQPRAPNAVQAPEGAETRAAIHESEAPNAPTGSVEDHWLFVVSDVGSLRRIPASETT
                     "
BASE COUNT         1075 a         1976 c         1841 g         1167 t
ORIGIN      
        1 cagatgctcc acccgcccct acatggaccg tatttggccc agagcctgcg gtcgagagcc
       61 cagcaccacc tcccatgaca ctcgaagcac cgccgttccc ttgggcagtc ccaagatttg
      121 acatgccccc gatcgtctca cctgccgcgc cgccggccga cttcacccct ggatctgatt
      181 gactgccgct acaggcaatc aagaattgcg ccacgaacaa cccgcagttg gcggcaattc
      241 gagccactct acacagctgc ggggactgca gggcctgact cggggtgggc tttcgtgcgt
      301 cgcagttctc gcgcagcatg acccacgagc attcaccggc gcgcgcgcct cgtcacgcca
      361 attcaaccgc cccgttgact agcttcactc ggttcctatg cccgcggacc gcatgttgtt
      421 ccagaatgtg cgactgcgcg tggctcgacc accggtcagg accccgctcg cttcgacgtg
      481 ccaagcgcct ggtcgcgctc aacctcggta gcgtagaact gcaaccgtga cgtcatcgtc
      541 tctcgatcgt gtccaattgc tgacttcgga catgatcgcc tcgcgaatgg catacgcgct
      601 cttctgccgg tgtgcctcga agacgcggcg caaacgctcc accccaaaca tctgcagatc
      661 ctggttcatg gcctccgtca cgccatccga gtagagcagc aacagatctc ccggatcgag
      721 ctgcagctcg tattgaatca tcgcctctgg ggacagctcc ttggtgatgc ctgcccagac
      781 gcccggcgtt tggatcatct cgctgctctc cgcgcaggtg cgcagtagaa tcaagtcttc
      841 gtgcgcgccc gccatcagca cacgaccatc tgtccagtat cgcatcaacg tcaacgtagc
      901 gtgttcgttc tgacccaagc gcgtgcggac attgtcgaag agcaccgaat tgacatcaca
      961 ccagagttcg accggcgagg cggtggctcc gcgctgcgca gctgctgcca cgatgctttg
     1021 gatcatgagc gccaccagtc ccgtcggaag gccatgtccc accacatcac caatgcccag
     1081 ccagcaacct tcggcggtgg gcaacacgtc gaagtagtcc cctcccacct cggtcgcggg
     1141 aatcatggcg gcgcttactt ccagcccggg cacgcgcaag tctttcggaa aaatggcggt
     1201 ctgaatgcgt tgggcaatgg ccatctcttg cgctaaacgc tcgcgctcca ccgcctctcg
     1261 gcgcgtcacc tcggttacga ccgtgcggag cagcgatacc gctttgaccg tggccgctat
     1321 cggagcgcag atctggtgat agcttccggg ttcgccgccg ccatggggca gaatgcagaa
     1381 tcccagcgtc tcattgccga agatcatcgg cagcacgacg taagtggtcg gccgagccaa
     1441 catttgctgg gtcggcaaca ccaagccggt ggtgaactcc agtgggcctc gtgccgtgtc
     1501 gccttgctcg ttacacggca ccaccaatct gctcaccgat cgcgcgtcta ccttctcgcc
     1561 ggttagtgtg acccaacatc gttccaggcc gagggacccg aggtgcggcg cgagcccgcg
     1621 gctcagcgcg tcgtagtcca atgccgtgcg cacggtattg ccgagcgcct ctagggcgtg
     1681 catttggaca tcattctcga tgcgcctacg cccctgcaaa cgttctgcat gactgccaat
     1741 cacgatatgc gcgcgttcaa tcagggactc gaaccggaac cactcggaga tgtccgtcgc
     1801 tcccgagcgg atcgctgccc cccgcagcgc ggacagcgcg ccatgccagc tcttggcatt
     1861 ccccagacgg gcgctgtagt ggagaaggcg ctccaacact ccgacgaatg cacccccgtc
     1921 gctcccgaga tcttgcagta gcgcgtcaat cagctctccg ctccagccga gctggtcttg
     1981 gaactcagcc accgcgtcga gccccggcat ggagttcacg agtgcggctt ggcacgaggc
     2041 gcgatgagag ccgagcagct cgagaaggtg agcctgagtc ttggcggtgg tcgggtccgg
     2101 cgaaagcgcg tgaccgagac aaccgcagga gtggcgcacg accaactccg tttgcaggta
     2161 gcccagctcg ggcacccctt cgcccgccaa ttgggccgtg atcaagcgca cggcggcgcg
     2221 ccctagctcc gcgatgggtt gccgaaccgt agtgagggcc ggtgtggtga atcgagcctg
     2281 gtcgctatcg tcgaaaccga tcagcgcgat ctgctcggga atggcgaacc cgcgtcgctc
     2341 tgcctcctcg agcaccccca tggccatcca gtcgttcgcg gccacgagag cgtcgatcgc
     2401 gggtgcgtct ggcgttccca gacgggccac cgcagccatc ccgctctcgc agctaaagtc
     2461 accctccaga atccaggacg ggtcgaccgc caatccacag tcgaggtgcg cccgctcgaa
     2521 gccgttccgg cgctgccgtg actcttctcc gggcccgctc aggaaggcca gccgtcggcg
     2581 gccatgcttc tcgatcaggt gtctcgtgga tgcgtaaaca ccacttgcgt tgtcgacgca
     2641 gatactcggc acgccagctt gagcgtagga gatgttgcaa cgagggagcc cggcaaaacg
     2701 tgcgaaagta gcttggacct ggggagagtc ctcccctcct cccagcgtgc cggacgcgaa
     2761 gatcacggca tcgacgtcac cttcgccgat cacggaatag gcgctattgc gtgggtccga
     2821 ggcgcacaac acgcccccga tcaggcaaat cgtatcgtag cccagctctt ggcagctctc
     2881 gtgaacgcct tgcaacagcg agtcttggta cgaatacccg agattgcgca tcagcagcgc
     2941 gacacgagta cgctggcgcc cccccgggga cgaattgatc gcgggcatgg ttggctcaat
     3001 cataatacac aagggttatt ggcgctcgac tgcgcgaccg cattcttcgc gtcgactttg
     3061 gcatcgcggc gcgaatgtaa gcctgccatc tcggattggt gcgaggcatg gttgaaccgt
     3121 gattcgtcgc gcgccacggt caagtcacgc ggcgccctcg aacacgatcg gaagtcaccg
     3181 tcgcatgtcg ggagtgggcg tgggtctgct tgttggtcgc ggttccgttg ctcgccgcgg
     3241 cgcttgcccg acacgtaccg ttgctccgga ccgctccgtt gatcgaaatc cgtgttactg
     3301 gcgccgccga gccagagacc acataacggg cgcgagctgg taagccagcg gccaaagcat
     3361 gcggcccggt cacgcaccgc cgtgccggtt gggcgcagcc cgctgcgtcg ctcagcccag
     3421 ctccacccac attggggtgc aagtcggctg gccttgtaca agaggctccc cggttcaggg
     3481 acatcaacca agaaactgca acagctagcg gggcgcgccc catcgcgatt ggcaggctgg
     3541 ggcaagcgca gtattggcta taacttgagc tcgggagcca aagtttggac gtgctgtcgc
     3601 ggggtgccgt tgcgcgcttt ggccctgtcg ttcctctgca ctctcgtgct gctaattgcc
     3661 catgcagcgt tcgagctggg tggcgcgggc agcgccgctc tgagcctgag tttcgttccc
     3721 ggtttactgg cgctgctggc gttggggccc gccctgggtt gggccgtgag cggcttgatg
     3781 ctcgcgagca tggcgtggtt gttcgtcgtc cgtcccccgc ttctgcgcta tgatttgctg
     3841 cgcttcatcg accagagcgc gatggtggtc ttcgcagccg ggttggcgca cgctctgcat
     3901 cgcagtttcg gctggtacga aatggcaatc gctcgccgcc aagcggtgct gctggatatg
     3961 caggaaaagc gaacaaacat cgcggccgca atcttcgagc agctggatcc attgtcctcg
     4021 caactcgttg ctacgctgtc cccgcccacc cccgatcgcc aagcgatccg aacgcttttc
     4081 tcacgactga tcgaacatct tagacgcgcc aaagagctcg cccggacgga cgaaaccgac
     4141 agcccggatc tccaagaacc cgatcgggac caactcattc ggtgccgaac gatgcgagtg
     4201 tggctgaggc tcggcgcgtt cctgatggcc tttttcgtga tccgcaacct agtcgccggt
     4261 acgccattcc taccgtccgt cttctcacta gcattttgcc ttttcttcga tatctggctg
     4321 ggcagctcgc aatgcagcaa atacttggaa tcgaccgcgc tggcaattgg gctatccgca
     4381 tctgggccca tgatcgcgca catccacgct tacggtggaa gagccgacgc tccgccgcta
     4441 gtggtcacgc ctggaatcgt gctgttcacg gccctactga gccaaggccc cgcgacttgg
     4501 gcggtggtcg cactgaacgt agccgtgctc gcttgggtcg gtctcggcag tgaaaccgat
     4561 gcaacgggct ccagccgact cacggacttg gccctcagct tcctggtaat cgtggtcgcg
     4621 ataaggagtg tcttcgcgct tcgccgacgt tacgccctcg cgctgttgga gcaagcccaa
     4681 gcgctggcgc gggcgttgcg gcagcatcgc cgtttggcgg ggactctatt tcacgatgtg
     4741 agtaatcact tgcaggcgct gagcctgcat ctggaagtcg agagcacccg agcagacctg
     4801 gatcatgcct tatccctgag ccgtcgtgta caacgcttga ttagactatc gaaggagttc
     4861 ctcctcaatc cgcaagaagg gccgtcaccc gcgctcggcg cggtgactct ggatgaagcg
     4921 gtcaagctgt tgctggaggc ctttgcgccg cgcctcggag ccaagcaggc gcagctcttg
     4981 gccggccccg gactggatct tcgcgtccgc gcgcaacccg agttgctgat cgagtccgta
     5041 cttgggaacc tattgagcaa tgccatcaag ttttctcctc tcggagcgca gatcacactg
     5101 aaagcggagc gcatcggacg aagcgtgaga atcgtcatct gcgactccgg ccgcggcatt
     5161 cccccggaag tggtcaagcg tttgggacaa gactgcgtgg tccccagcgc gctcggcacg
     5221 gctggggagc gcggtcaggg ttacggactg cagctggtcc acgagcacct gcaacgtatg
     5281 gggggccggc tggagctcag caatcgacgg gaggggggta cggaggccgc ggtctggctc
     5341 gagacggcat aggcgtagtc gcccgcaggg aacctttggg agtttgcgct gtcccaccgc
     5401 aagcatgagt ttgaggcttc gttccggccg ccttttgggg tgcctagttt ctgcgttatt
     5461 cgtggcgtgc caatcacccg tgctgcgggc gcccgcggcg ccagccggcg ccgaagggag
     5521 cgtacttccg ctggcacgtg ttcgcctatt cgagaacgga attggctact tcgagcgctc
     5581 cggaacgctc accgccacgg cggcgctcct gcctgtaccc gcgtcccatg tcgatgacgc
     5641 actaaagaca ttagtcgtgc tctcgaacag ctcgatgacg gtctccggga tcgagttcgc
     5701 gagcttggtg agcggtggga cggcccgtgc tttggcaggg ctgcccttga gcgatgagac
     5761 gcctctcggc tacggagacg tcctgagcag cctcgaagga tatcgcgtgc gcctcaccac
     5821 caagagtggt gcccagttct ccggtaggtt gatcgacgta cagcgcttcg agcgtgccgc
     5881 cgccacgtgt cagccccgcg caccgaacgc cgtccaagct ccagaggggg cggagaccag
     5941 agctgcgata catgagtccg aagctccgaa tgccccaact gggtccgtag aagatcattg
     6001 gctgttcgtg gtgagcgacg tgggtagctt gcgtcgaatt cccgcctccg aaaccaccg
//
LOCUS       DASTKU010000328         6234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_732018, whole genome shotgun sequence.
ACCESSION   DASTKU010000328 DASTKU010000000
VERSION     DASTKU010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732018"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            548..913
                     /locus_tag="VFQ61_11640"
     CDS             548..913
                     /locus_tag="VFQ61_11640"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9955151.1"
                     /translation="MATKGTKRIGQLIRSARMHRGLTQKDLARKLGVRSGSYVGMLES
                     GLCKGIGISRLAEIAGYLDVDGLDLYRWMAAAGHLPPNLFGALMKRPDRWDDVMSLLG
                     PAVGGSRAPRSSRRPRARS"
     gene            1891..4509
                     /gene="clpB"
                     /locus_tag="VFQ61_11645"
     CDS             1891..4509
                     /gene="clpB"
                     /locus_tag="VFQ61_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HET9955152.1"
                     /translation="MRMDRLTTKSQEALRAAIDQASRRGNPEVIPEHLLLSILEQEGG
                     VGSALLQRAGADPKAVASDFSKRLEGLPRVSGGAEPSLGRRAVPLMNHAEDEAKRLKD
                     DYVSVEHFLLAGAKHDKDVQAVFDRNGVSYDKLVRALAEIRGSQRVTDQDPEGKFQAL
                     EKYTRDLTALAKRGKIDPVVGRDDEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIAEGI
                     AHRIANGDVPESLKDKRILALDLASMVAGSKFRGEFEDRLKAVLKEVEASSGAIILFI
                     DELHTLVGAGAAEGAMDAANMLKPALARGELRAIGATTLDEYRKHIEKDAALERRFQP
                     VMVSEPTVEDTIAILRGLKERYEVHHGIHIQDGALIAAAKLSQRYITDRFLPDKAIDL
                     VDEAASQIKMQIDSLPFDIDQIERKLLQLRIEEQALNKERDKGSQARLQDLKKEISEL
                     NTERDRMRAQWMREKELISKIRDRQARLEELRLEEERARRVGDLGKAAEIQYGKLPEL
                     ERAIEAERAELGEVQKNGAYLKEEVGEEEIAAIVSKWTGVPVSKMLEGEVQKLLHMEE
                     NLHRRVVGQAKAISAVANAVRRSRAGLGDTGRPIGSFLFLGPTGVGKTETARALAEFL
                     FDDERAMIRIDMSEYMERHAVSRLIGAPPGYVGYDEGGQLTEPVRRRPFSVILFDEVE
                     KAHPDVFNTLLQVLDDGRLTDGQGRTVDFKNTVVILTSNVGTQELSEIEERRDLDEAT
                     RAQLAQSVAMTALKQQFRPEFINRLDDIVVYHRLSREHVHRIVDIQLSRLAQRLALRE
                     IGLSLSDPAKDYLGEVGWDPQFGARPLKRAIQKHLEDPLAQRVLAGEFLPSDTVVVDR
                     GPEGLTFTRRAASSLN"
     gene            4595..4774
                     /locus_tag="VFQ61_11650"
     CDS             4595..4774
                     /locus_tag="VFQ61_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955153.1"
                     /translation="MFSKLLLLGLLGLLLTKVSLRTHLRELGKKLDRVVNATLIAIVI
                     VYFAQLLYMWLGSRP"
     gene            complement(4821..5564)
                     /locus_tag="VFQ61_11655"
     CDS             complement(4821..5564)
                     /locus_tag="VFQ61_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955154.1"
                     /translation="MAKVERALLRSLALLVIPVAAALLLVGLLIANRTSWSAQEIRHR
                     SDLAEQCDSALAEASRALESGLFETALARLESNRKCDGELEFDAARAEALAFLDRVLE
                     AESLVSSVLARSPHQPSGLRARAASSISRRQFQIAEASIRELDQVHPKHVSTLYLKAL
                     TAMRRAQYNAARTGFLGVLRANRRHVGARFALVVLTSQSGVSDEARHHLRELLEIAPA
                     DDPRRAQAERLIDAPVVSPNSRVLKLGSD"
BASE COUNT         1258 a         1863 c         1976 g         1137 t
ORIGIN      
        1 tgatctgaca ggcctactgg ccgctctgct cctgccgagt gggtggctct cgtgggtcac
       61 acggctactc cgactcggag caccagacct cgactgcgtg aaagctcgcg cgacatccct
      121 gaggttgatg tcggggattc gcgatcgatc tcgagttacg cgggcattgt atggagtccc
      181 atccaaaagg gttggaacac caaccaagca actttctcgc gcgactcgcg tggtacgggt
      241 cataaaaata tcgaaattcg acacagtgat tggggcgcct cgcccgcacg tgagtgggcg
      301 acctttggcg tcagtgtggg cgaacatcaa tctagactgc actcccacgg gtggaatgga
      361 tgaattcatt cgtctggcga ctttcgccac tacccatgag gcaggagttg gagttagtct
      421 gccggtgctt gagagagaaa cgtcgagcaa tcgctcgaac ctctctcgtc gcggtaattc
      481 gaaagaaagg aggggccatc tggcccgccc ggcaagaagc accggctcag atggcacggg
      541 aatatccatg gctacgaaag gcacgaaacg cattggtcag ttgatccgat ccgcccgtat
      601 gcaccggggc cttactcaaa aggatctcgc tcgtaagctc ggagttcgct ccggcagcta
      661 cgtggggatg ctggagtccg gtctgtgcaa ggggatcggc atttcgcggc ttgccgagat
      721 cgcgggctac ctcgatgtcg atgggctgga tctctatcgc tggatggctg ccgctggtca
      781 tctcccgccg aatctgttcg gtgcgctgat gaagcgcccg gatcggtggg acgacgtgat
      841 gtcgctgctc ggtcccgctg tcggaggttc gcgagcaccg cgttcttcgc gccgtccacg
      901 cgccaggagt tgagtcgtgc cgtgtgggct gcccaaagcg aaaaaatagg gcagcccaca
      961 cgttttgtca gaacgccatc tcctgcttcg ggcatcgccg tggcggctcc taccgcgctc
     1021 atgaccacgc gcagatccga gctgcatcag cgcttcaccc gcggtcgacc gtgcaccacg
     1081 ccactaccca tccggcgaag tcaaaaagcg agtgcaccgg tcgagccccg cggacctgca
     1141 aatcacactc ggctattgga gcggtgagct cactcgacgg ggctctgtta tcgaaccggg
     1201 ttattgtatc gatccgggtt attgagcgcc gggcttcatc ggagcagccg aaagctggat
     1261 aaccgcacct cccgaggaca gcgtctggag gagtacagct cgagagtagt agagcggcta
     1321 gcgcggcaaa tcctcgcagc atccggacca ggttcgaaga accgtgatcg ctcctcgtcc
     1381 ggagagcctc agggacggct atagttttcg cctccaccga gtcgggagga aagggtagct
     1441 cgacgcgagc tgacgatttc tttcatttcg taccctcaat cgtcagcact tagagcgaaa
     1501 cctcgtattg actgtttcgc tctgccgctc aagccctggt gcgagccgtc aagcagtcgg
     1561 gagggcagag tcgggagggc agagtcggga gggcagagtc ggcgaggcac ctcaggactg
     1621 gtgcgatgac ttcgccccta cgggcctgag aggtcattcg ggactcgtgg ggaccgctca
     1681 gggctcgatt gcacgccgta agcacgacga accgtgggtt gagcatggcg ggcaggccgc
     1741 ccccttgatt cggcgagacc ccgacttagg ttgggctggc acgaaacctg ccgttaacag
     1801 gacgctcacg cctacgttct ctcgcgtcgc tcttcgcgcg aacgcactga tcgcctgcac
     1861 ggcggagttt cgcccgacgg agtctccaac atgcgtatgg atcgcctaac caccaaaagc
     1921 caggaagcct tgcgcgccgc catcgatcag gcgagccgtc gaggtaaccc cgaagtcatt
     1981 ccggagcact tgctgctatc catcctggag caagaggggg gtgtcggcag cgctctgctt
     2041 caacgtgcag gagcggatcc aaaggccgtc gcgtcggact tctcgaagcg tctcgagggt
     2101 ctgccgcgcg tatccggtgg cgccgagccg agcctcggta ggcgcgccgt gcctctgatg
     2161 aatcatgcgg aggacgaagc aaaacggctc aaagacgact acgtgtccgt cgagcacttc
     2221 ctcctcgcgg gcgccaagca cgataaggac gtgcaagccg tcttcgatcg caacggagtt
     2281 tcgtacgaca aactagtgcg ggcgctggcc gaaatccgcg gcagccagcg cgtcaccgac
     2341 caggaccccg aaggcaagtt ccaagcgctg gagaagtaca ctcgagatct gaccgcgctc
     2401 gccaaacgcg gcaagatcga cccagtcgtt ggacgagacg atgagattcg acgcgtgatg
     2461 caggtgctct cgcggcgcac caagaacaat ccagttctaa tcggtgaacc tggcgtgggg
     2521 aagaccgcca tcgcggaggg cattgcccat cgtatcgcga acggggacgt gcccgaatcg
     2581 ctcaaagaca agcgcattct cgcgctcgat ttggcgagta tggtggccgg ctccaagttt
     2641 cgcggtgaat tcgaagaccg cctcaaggcg gtcctcaagg aagtcgaggc ttcgtccggc
     2701 gccatcatct tgttcatcga cgagctgcac acgctcgtgg gcgccggcgc ggcagagggc
     2761 gcgatggatg cggcgaacat gctcaagcct gcgctcgcgc gcggcgagct gcgcgccatc
     2821 ggcgcgacca cgctcgacga gtatcgcaag cacatcgaga aggacgcggc cctcgagcgg
     2881 cgcttccagc cggtcatggt gagcgagccc accgtggagg atacgattgc gatcctacgt
     2941 ggcttgaagg agcgttacga agtgcaccac ggaattcata tccaagatgg tgctctgatt
     3001 gcggccgcca agctctcgca gcgttacatc accgaccggt ttctgcccga caaagccatc
     3061 gatttggtgg acgaggccgc cagccaaatc aagatgcaaa tcgacagctt gcctttcgac
     3121 atcgatcaaa tcgagcggaa gctgctccag ctacgcatcg aggagcaagc gctgaacaaa
     3181 gaacgggaca agggcagcca ggcgcgcctc caagatctga agaaagagat ctcggagctg
     3241 aacacggaac gcgatcgcat gcgtgcccag tggatgcgag agaaggagct gatctcgaag
     3301 atccgcgacc ggcaagcccg tctcgaggag ctccggctcg aagaggagcg cgcgcggcgc
     3361 gtcggggatc tcggcaaggc ggccgagatc cagtacggca agctgcccga gctcgagcga
     3421 gcgatcgagg ccgaacgcgc ggagctcggg gaggtccaaa agaacggcgc ctatctgaag
     3481 gaagaggtgg gcgaagaaga gattgctgca atcgtctcca agtggaccgg tgtacccgtc
     3541 tcgaagatgc tggagggtga ggtacagaag ctcctgcaca tggaggaaaa ccttcaccgc
     3601 cgcgtggtgg ggcaagccaa ggccatctcg gcggtggcca atgccgtgcg gcgctcgcga
     3661 gccggcctgg gagatacggg tcgtccgatc ggtagctttc tttttctggg ccctactggc
     3721 gtaggaaaga ccgaaacggc gcgggcgctc gcggagttcc tgttcgacga cgagcgcgcc
     3781 atgatccgca tcgatatgag cgagtacatg gagcgccacg cggtctcgcg cttgatcggc
     3841 gcaccgcctg gatatgtcgg ctacgacgaa ggtgggcagc tgaccgaacc ggttcggcga
     3901 cgtcctttca gcgtgatctt gttcgacgaa gtcgagaagg cccaccctga cgtcttcaac
     3961 acattacttc aggtactcga cgatggccgc ttgacggatg gccaaggccg cactgtcgac
     4021 ttcaaaaata cggtggtgat cctgaccagt aacgtgggta cccaagagct ctcggagatc
     4081 gaggaacgtc gagatttgga cgaggccaca cgggctcagc tcgcgcagag cgtggccatg
     4141 accgcgctca agcagcagtt caggccggag ttcatcaatc gcttggacga tatcgtcgtc
     4201 tatcaccgcc tgagtcgcga gcatgtgcat cggatcgtcg acatccagct ctcccggttg
     4261 gcgcagcgcc tcgcgctgcg cgagatcggg ttgtccttga gcgaccctgc caaggattac
     4321 ctcggtgagg tgggctggga tccgcagttt ggcgcccgcc cattgaagcg ggcgatccag
     4381 aagcacttgg aagatccgct ggcacagcgc gtcctggcgg gtgagttctt gccttcggat
     4441 acagtcgtcg tcgatcgtgg acccgagggc ctcaccttta cgcgacgcgc tgcctcctcc
     4501 ctgaactgat ccgaaggaac cccagcgagc gcggtgtcag aaacagcgtc cgcgggcttg
     4561 accgtccgcg gcgctgttgg gagcctgaga gcaggtgttt tccaagcttc tgctgctcgg
     4621 cttgttgggc ctgctgttga ccaaggtttc tctgcgtact cacctgcgcg agctcgggaa
     4681 gaagctagac cgagtcgtga acgcgaccct catcgcgatc gtgatcgtgt acttcgctca
     4741 gctgctgtac atgtggctgg gatctcggcc gtagatccgc acccgagctt ggtcactgcc
     4801 gagcttggtc actgccgagc ctagtcactg ccgagcttga gaaccctcga attcggactg
     4861 accacgggcg catcaatgag gcgctcggct tgcgcgcggc gggggtcgtc ggcgggagcg
     4921 atctccaaca gctcgcgtaa gtgatgccgt gcttcgtcgg atacgccgct ctgtgaggtg
     4981 agcacgacca acgcgaatcg tgcacccacg tggcgccgat tggcgcgcaa tacacccaga
     5041 aagcccgttc gcgcagcatt gtactgggcg cggcgcatgg ccgtcagtgc tttcaagtaa
     5101 agtgtgctga cgtgtttggg gtgcacctga tccaactcgc gaatgctcgc ctccgcgatt
     5161 tgaaattggc gtctcgagat ggagctggct gcgcgagcac gcaagcccga gggttgatga
     5221 ggcgaacgtg cgaggaccga gctcacgagc gactccgctt ccaaaacgcg gtccagaaag
     5281 gccaatgctt ccgcccgcgc ggcgtcgaac tcgagctctc cgtcgcactt gcggttcgat
     5341 tccaagcgag cgagcgccgt ttcgaacaac ccgctctcga gtgcgcgtga ggcctctgcg
     5401 agcgctgagt cgcactgctc tgctaaatcc gatcggtgcc tgatttcctg agccgaccac
     5461 gacgtgcgat tcgcaatcag gagcccaact agcagcaagg ctgcagccac cgggatcacg
     5521 agcagagcga gggatcgcaa gagggctcgt tcgaccttgg ccattagagc tcagctccgg
     5581 gcttaccgcg agcaagggat tgtctcggtg gggcgagggt gtactcgagg acagggttgg
     5641 acacgcgcgg gctccaagat ccacggtctg gcgcccctgg gcaatggcag acgcaacact
     5701 cccgtcccaa aggtccaatc gggcggtgaa cgcgctgcga actccgtcca gtcgacatca
     5761 cagcggcgac atccaccagc tttgccagcc caggagagag ctgaccgtcg aggtcatacg
     5821 ccgcgcgggc gaagccctga tcgggatcag tgaggcgggt caggcggatc cgctagcgaa
     5881 cccgagctcg ttctcaatgg ccaagtttcc gaagagcgcg cttcgattga acctaagcgg
     5941 cgtggatcca caactgctcg tctcggtact cttagtgcgg gtgcaaccgc tattggcagc
     6001 gccgcgatcg gaccggttgg tgaccggcag tgtcgtcaca ggacgtagtc aaccgaaccc
     6061 attttgcggc ttcgcatgct ggccacctga tcacctgatt cgctgcctta cacgacgcgc
     6121 aatgttcgag aaacgactac gggcccagaa aaacacggga aaaagccgca caaatcggac
     6181 cgcagaaacc catcgtgctc tcgttgacct tgcttccggg caatagtgct ctgc
//
LOCUS       DASTKU010000329        13283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_732538, whole genome shotgun sequence.
ACCESSION   DASTKU010000329 DASTKU010000000
VERSION     DASTKU010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13283
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732538"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFQ61_11660"
     CDS             <1..229
                     /locus_tag="VFQ61_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1585 domain-containing protein"
                     /protein_id="HET9955155.1"
                     /translation="GLGELSEILSKDERLEACVVKKMMTYALSRELVATDAGYVAQIQ
                     AAWSGGKKNLTALLEQIVLSPPFRSRRGEAP"
     gene            275..1678
                     /locus_tag="VFQ61_11665"
     CDS             275..1678
                     /locus_tag="VFQ61_11665"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9955156.1"
                     /translation="MSSIHRRRFLQGAGIGLALPWLETLAPRDARAQAQPTKRRYIML
                     YFPNGTADFWRPAAAGTGDAWKLSPILEPLAPVKKYLTVLSNVSNYAPFGGHVEPSHS
                     NLGAATWTCVKPSGDGNANSGISVDQVIANRIGSSTRLASLQVGLSTLDSYTDGLPGQ
                     HSRSMSWKSANQPLYKIVNPQAVFDRLVSNVSSGSGTEDAAAARRRALKRSALDYVLK
                     DASELQKKLSVSDRARLDQFLSSVRSLEKRVADSGVVAQPLECGSIARPSEAYTVGNV
                     GPGYSREKHASLMIDLVVMAIRCDVTRVVSFMLDDARSDFAYDFLKERKFTDAGSTEG
                     SGTVGGYHGLQHAGDRNNGFATIGWWNAQKAAQLAQQLQQIDEGAAGNALDNSVITFA
                     SGMKGGNHDARELPIALIGGGGKGASGTVLKTDRHVAFANETRLADVHLTILKQVFDC
                     PETSFGASSGILPELVA"
     gene            1680..2927
                     /locus_tag="VFQ61_11670"
     CDS             1680..2927
                     /locus_tag="VFQ61_11670"
                     /inference="COORDINATES: protein motif:HMM:NF027798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,3-glucanase family protein"
                     /protein_id="HET9955157.1"
                     /translation="MARGARLRLSVCSSVLVVLACGSSSGSPGSSGGTPPSPGGTSGA
                     GSVSSSGGVAFGSGGSESSGGAPQPSRAGAGGATVAHTGGASSRGGSASSGGSLTAGE
                     SPSRGGASQVGGALHSGGTLHSGGSASTAGSGSSGGVYVVERDPNAPGILIHVENRCP
                     FDLWIRGAGSGAVLQPENARLSTGQAQDYLAPDQWSAARVTAYGEAPNAQGQPQHELD
                     KVEMTLGKKIINYNITYVDWLALPVELRGIGSGSDCRVVGCYASVADVAARCPAGLMS
                     GERCLSAGNFCSRSENQSSAFCTALDEKIAACAADPKSFPGCEGAQGAKTPEAYGCSG
                     FFAQSPKWCAALNRGMLEAPDDGDITKYYRQPPFNAYAKWVHDVCPGIYAFPYDDYGK
                     TNESGFHACTGGTQLNITFCPNG"
     gene            3117..3761
                     /locus_tag="VFQ61_11675"
     CDS             3117..3761
                     /locus_tag="VFQ61_11675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9955158.1"
                     /translation="MTQRSPTLTHADGMLVEGVEDPRANFREVYDTHFAFVWRAVAHR
                     GVPRSALDDVVQEVFLVVHRKLGEFEGRSSLRTWLAGIVRRVVADHVKKRSNRAVAQE
                     VLEEHPAESGAEPGAQAERRAAVALVEELLQKMSAVQREVFVLYELEQMTTREIAALT
                     GSNENTVQTRLKAARKVFQQGLARHRADFAKSSGASRGSAAPAGIAASKDLEGA"
     gene            3758..4498
                     /locus_tag="VFQ61_11680"
     CDS             3758..4498
                     /locus_tag="VFQ61_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955159.1"
                     /translation="MMELDSDARRLLELAREARTPTLADKIRIESALSQALQAAAAGG
                     ATGSVATGGGAAASTTKSTVGLATAKWLAALSVPALVVGALQLRNPETGEPSGIGAAS
                     VVATRRVASNQVVVTPSAAHSTPQTDPGFEPAVASHGDFVSRNAGSRARSASKPSAPD
                     SLNQELDLLHAAQKAWRSGQAKQALVLIERHQREYPRSVLGLERDALRILSLCAAGRE
                     GEAKALARRFNGRRSPLGAAVEQSCAAD"
     gene            4524..5561
                     /locus_tag="VFQ61_11685"
     CDS             4524..5561
                     /locus_tag="VFQ61_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955160.1"
                     /translation="MRRLPPLVLLLWWFALPNSARAEERLPLELDWEAPAECSNADSV
                     HAELARIAHVRAGREVQPLAAKVRIRKLGPRYRLLLHITQGGSSSERTLAASECRALQ
                     REVTLVLALAFGEGVELTTDEPAEVRERHGEDEPQGTRIDEPTPPVAAPPPEPAPVQV
                     RTPKPTPPRRSPAPANRFRWDWLAGGGALWRALPVTSPVAFLGADVGFDSWWLQPRVQ
                     LIPGVNRELPRGIQTRYDGLGATLAGCSGAALASLRLATCLSLSATALRGRASGAVEE
                     ENAIAPWYTAGAHVSLLWPARGWLGGGVEGAFSYSLSRPRFRINGLADVHTVPRVAPA
                     LSVYLRVRLEP"
     gene            complement(5583..6320)
                     /locus_tag="VFQ61_11690"
     CDS             complement(5583..6320)
                     /locus_tag="VFQ61_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955161.1"
                     /translation="MILRRAAAAYSFGLLFAGCQDNPYTIGHFRDDLCATREQDLVCS
                     GFEQPDLSDWGEQVLVNAGSLAQTEQVSRRGRGALLAGSTDRDSAAAVAKEFSSVTSG
                     SAFLRVFMLIPSGVETKTTNVLFLGDQPAPDPFKGIDFNFESDAPEIFVPENDPVRFT
                     STSLVVPRDRWFCFEADVLVSEAAGAVQIRIDGAVALDQTGLDTLPPQGVHLIRAGVD
                     WSSKQTTPFAIYMDDLVLSAAPVGCGD"
     gene            complement(6388..6645)
                     /locus_tag="VFQ61_11695"
     CDS             complement(6388..6645)
                     /locus_tag="VFQ61_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955162.1"
                     /translation="MAQSTFGTPYARRSWSSLYPEPERPVDEEYVRSVKARRMGARLL
                     FGLAVAGVLTLLWFEAKALSAAPAPLTSLSSGTYVSAEAAH"
     gene            7097..7990
                     /locus_tag="VFQ61_11700"
     CDS             7097..7990
                     /locus_tag="VFQ61_11700"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9955163.1"
                     /translation="MSWNYAGWSGIVYSKNANSKELARHGLAAYARHPLLSTVEIDRS
                     YYEPLPAHAFADFAKQVPDSFRFVVKAHEDCVVRRYPQHARYGKKRGQVNSLFLDAGY
                     ATEHVVAPTVEGLKHQLGVLLFQFPPQDVGSPLGFARQLEAFLRRLPAGVVYAVELRN
                     RELLGRAYAEALAATGAVHCHNVWGEMPALLEQIRALPPVARRPLVIRWLMRPGVDYQ
                     SAGARYSPFDRLVDEDVSHREQVARIVTRASEHGVPAFTLIDNKAEGCAPESAARLAR
                     CIVEKKRTATAQSEPRPVKLE"
     gene            complement(8013..8948)
                     /locus_tag="VFQ61_11705"
     CDS             complement(8013..8948)
                     /locus_tag="VFQ61_11705"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET9955164.1"
                     /translation="MKDALTRIAIFYDGNFFHVVSNYYAYNHARAARLSISGLHEFVK
                     KRVAYHEHVDVRLCQIVDVHYFRGRFSAQESSEAGKLLAERLFEDVLVREGVTTHYLP
                     MGYAGAEKGIDVWFALEAYELAIYKRLDVCVLVAGDSDYVPLARKLNALGTRVMVLGW
                     DFEYTDQQGRLRTTQTSPALLAEVTYPILVSNLIEDKTQKDLVAELFLPTRDPSRAAA
                     PPSVPTPGPSRTTKASERQQGKIQNLVVREAENKRFGFIAPDAGGENIWFGESDIDDV
                     SFDALQRGDRVSYELGSNWLGACAKHVIRVDPDET"
     gene            complement(8987..9697)
                     /locus_tag="VFQ61_11710"
     CDS             complement(8987..9697)
                     /locus_tag="VFQ61_11710"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9955165.1"
                     /translation="MRSFFSARGTRAPAWGVVWLVTLGSGCSRKPAEPAEPTAAQRAP
                     VSAPSTPSALGSARLGAPAPDFELADTEGQPVKLSSFRGKRVVIEWFNPGCPFVKASH
                     TKGSLVNSAARQQANGVVWLAINSAAPGKQGSGAEVNREARATYNLQHPVLLDETGQV
                     GRAYGAAHTPHMFVIDQNGILVYRGAIDNSPDGEGESPSGGKLVNYVEAALTDLGAGR
                     AVSTPETEAYGCTVKYGS"
     gene            complement(9798..10901)
                     /locus_tag="VFQ61_11715"
     CDS             complement(9798..10901)
                     /locus_tag="VFQ61_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955166.1"
                     /translation="MNFKPLLGTLCLTLLASRSFAQPSPNSTPDLETAKLETAARSAN
                     ERSANERSATQHAELGRRLAFAGDLDAALLEFQSSYELIPGGPILYDIASVHLARGDY
                     VAAIAFLRQYLDESTAETSGGDNAKLESETQRQLRNRAMRDLEHAQTRVATVRLEGDL
                     TGAELLVDEQSRGESLDSVVLVNPGTRRLLIRKRGFKPVRTVLTLSMGESRLLSVSLQ
                     PSASARPNTDSDAPSRTRAVFLPRDQAARSAAPVGSPTNLGLWVSVVATGVAAGATLA
                     SSVLTQRASKEYTQASQASPSSPSRISETRGALKRTALVTDMCGALTALGVGATIYFA
                     VTGGREPVDRRGARLWTIRTGPAGLGWQVQGLF"
     gene            complement(10991..12850)
                     /locus_tag="VFQ61_11720"
     CDS             complement(10991..12850)
                     /locus_tag="VFQ61_11720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9955167.1"
                     /translation="MSHPKLPAIEKSSALAKSPLVERGPMRLGRYELVQKLTSGGMAD
                     VFLAHQPGPFSAGKLLVIKHLRGGMVDDDEFVQMFADESRIAVRLSHPNVVQTYEAVV
                     EGDDYYLVLEFLDGKPLHQLLNQVSRARMPLALHLWILRQVLSGLHYAHELLDFDGTP
                     MSIVHRDVSPSNVFVTYEAGVKLLDFGIAKSLGAMSATREGVIKGKLGYAAPEQCLCK
                     PVDRRTDLYAVGVMLWEAIAGQKRPMGQTQASAYQARIQGTELRIEQVVPSAPRELIT
                     ITNRALEQRPEARFQSALEFGQALDHYLRGIGFENGQEYLRSFMHEQFGSEMEEMRRR
                     IEDHLGHSRAHKTQRALAADRAALSATSPESLSQPIPIHELSAEGSTLKPPLLESAAP
                     VRLSARPRGWALAAAGGAVLLILAGTVASRGGGTKTAPSSSSAAAHESHAVTLVPARP
                     AMEAASSTRSTVVDGAVVMGQVLVSIEAKPSSAELRLDGRRISNPYRAFHGKDARSHV
                     LTVSQRGYQTSQRDLLFDRDLELTLSLTTQASLRAPANPRLSVKESGRNSRSTATRQA
                     SAPVVAPQNSALPSEAEAKARPASKNDRLQPGDVLRASDRPSREIDNSDPYAL"
BASE COUNT         2375 a         4023 c         4406 g         2479 t
ORIGIN      
        1 ggggctgggc gagctctccg agattttatc caaggacgaa cgtctcgaag cctgcgtggt
       61 gaagaagatg atgacttacg ctctgtcccg cgaactcgtc gcgacggacg cgggctacgt
      121 tgcgcaaatc caagccgcct ggtctggcgg aaagaagaat ctcactgcat tgctcgagca
      181 gatcgtgctg agcccgccgt ttcgctctag gcgaggagaa gctccgtagg gctgccgtcg
      241 gaacccttct cgagatgaca cggagaacat gagtatgagt tccattcatc gtcgtcggtt
      301 tttacaaggt gcaggcatcg ggctggcctt gccttggctc gagactctgg cgccacgtga
      361 cgctcgcgct caggcgcagc ccacgaagcg ccgctacatc atgctgtact tcccgaatgg
      421 aacggcggac ttctggcgac cagccgccgc gggtacgggg gatgcttgga agctctcacc
      481 gattctggag cctctcgcac cggtgaagaa gtacctcacg gtgctgtcca atgtctcgaa
      541 ctacgcgccg ttcggcggcc acgtcgagcc cagtcattcg aacttggggg cggcgacctg
      601 gacctgcgtg aagccgagcg gagacggcaa cgcgaacagc ggaatctcgg tcgatcaggt
      661 gattgccaat cgcatcggga gctcaacgcg attggcttcg ctgcaggtgg ggctctcgac
      721 gctcgactcc tatacggacg gcttgcccgg gcagcactcg cggagcatgt cctggaagtc
      781 ggcgaaccag ccgctgtaca agatcgtcaa tccgcaggcc gtgttcgatc ggctggtatc
      841 caacgtctcg agcggctcgg ggactgaaga tgccgccgcc gcgcgccggc gcgcgctgaa
      901 gcggagtgct ctcgactatg tcctgaagga tgccagcgag ctgcagaaga agctcagcgt
      961 gagtgatcga gcgcgcttgg accaatttct gagctcggtt cgtagcctgg agaagcgcgt
     1021 cgcggactcg ggagtcgtgg cgcaacctct cgaatgtggc tcgatcgcgc gccccagcga
     1081 ggcctatacg gtgggcaacg tggggcccgg ctacagccga gaaaagcacg cgagcctgat
     1141 gatcgatctg gtggtgatgg cgattcgctg cgatgtgacc cgcgtcgtca gtttcatgtt
     1201 ggatgacgca cggagtgact tcgcttacga ctttttgaag gaacgaaagt tcactgacgc
     1261 ggggtcgacc gagggcagcg gcaccgtcgg cggctatcac ggcttgcagc atgccgggga
     1321 tcgcaacaac gggtttgcga cgatcggctg gtggaacgcg caaaaggcag cgcagctcgc
     1381 gcagcaactg cagcaaatcg acgaaggggc ggccggtaac gcgctcgaca acagcgtgat
     1441 cacgtttgcg agtggaatga agggaggaaa ccacgacgcc cgcgagctgc cgattgcctt
     1501 gattgggggc ggcggcaagg gcgcgagcgg caccgtgctc aagaccgatc gccacgttgc
     1561 gtttgccaat gagacgcgcc tggccgacgt tcacttgacc attctcaagc aggtgttcga
     1621 ctgcccggag acgagcttcg gggcaagctc tgggattttg ccggagttgg tggcctgaca
     1681 tggctcgcgg agctcgattg cgactcagcg tttgctcgag cgtgttggtc gtgctcgcgt
     1741 gcggctcttc gtcgggttcg cctggttctt ctggaggaac cccgccgagc cctgggggta
     1801 ccagcggggc aggctcggtg agcagcagcg gtggagtcgc gttcggcagt ggcggctctg
     1861 agagcagcgg cggtgcccca caacccagcc gggccggggc cggtggggct acggttgcgc
     1921 acaccggcgg cgcttcgagt cgaggcggct ccgccagctc gggcggatcg ctgactgcag
     1981 gggaatcgcc gagtcgcggg ggggcgtcgc aggtcggtgg cgcactccat tcgggcggca
     2041 cgctccattc gggagggagc gcgagcactg cgggttccgg ttcgagcggt ggagtgtacg
     2101 tcgttgaacg cgatccgaac gcccccggta ttctgatcca cgtggagaat cgttgcccat
     2161 tcgatctctg gattcgagga gcgggcagcg gagccgtgct ccagccggag aatgctcgac
     2221 tgagcacggg tcaggcgcag gactacctgg caccggatca atggtcggcg gcccgcgtga
     2281 cggcttacgg agaagccccc aacgcgcagg ggcagccgca gcatgaactc gataaagtcg
     2341 agatgacgct cggcaagaag atcatcaact acaatatcac ttacgtagat tggctggccc
     2401 tgccggtaga actgcgcggc atcggctcag gctccgattg ccgcgtagtg ggttgttacg
     2461 cgtcagttgc agacgtggcg gcgcgctgcc cagcaggtct aatgtctggt gagaggtgct
     2521 tgtccgccgg aaacttctgc tcacgaagtg agaaccagag ttcggcgttt tgcacggctc
     2581 tcgatgagaa gatcgcggcg tgcgccgccg accccaagag ctttcctggc tgtgagggcg
     2641 cgcagggcgc aaaaactccg gaagcatacg ggtgctctgg attcttcgct cagagcccca
     2701 agtggtgcgc ggcgctgaat cgcggaatgc tggaggctcc ggatgacggc gacattacca
     2761 agtattatcg tcagccaccc ttcaacgcct acgccaagtg ggtgcacgat gtttgccccg
     2821 ggatctatgc gtttccgtat gacgactacg gcaagaccaa cgagagcggg tttcacgcct
     2881 gcaccggggg cacccaactc aatatcacct tctgccccaa cggctgaacg ctagtctacg
     2941 aacaggtcga tacgtgatga ctgtcgcagg tttcaggaca aagacgccga actcgacgtg
     3001 ccaacgcgag ccggcgtgag cgtctggacg gtacgatgct accttcccag ttgacccgtg
     3061 cggacacggc gctagccccg actccccgcg aaacaccgcg gcgcgaagca aagcgtatga
     3121 cccagcgaag cccgactctc acccacgcgg acgggatgct cgttgaagga gtagaggacc
     3181 cgcgcgccaa cttccgggaa gtttacgaca cgcactttgc tttcgtgtgg cgcgcggtgg
     3241 cccatcgagg ggtgcccagg tccgcgctcg atgacgtggt tcaggaggta ttcctggtag
     3301 ttcaccgcaa gctgggcgaa ttcgagggcc gctccagctt gcgcacgtgg ctggccggaa
     3361 tcgttcggcg ggtggtggcc gaccacgtga aaaagcgcag caatcgagcg gtggcacagg
     3421 aggtcctgga ggagcatccg gcagagagcg gggccgagcc gggggcgcaa gccgaacggc
     3481 gggcggcggt cgcgctggtg gaagagctac tgcaaaagat gagcgccgtg cagcgcgaag
     3541 tattcgtgct ctacgagctg gagcaaatga ccacccggga gattgcggcg ctgactggca
     3601 gcaacgaaaa cacggtacaa actcggctca aagcggcacg taaagtgttt caacaggggt
     3661 tggcgcgcca ccgcgctgat tttgcgaaga gcagcggggc ttccaggggt agcgcggctc
     3721 cggcaggtat tgcggcttcg aaggacttag agggagcatg atggaactcg actcggacgc
     3781 tcggcggctc ctcgaactgg ctcgtgaagc gcgcactccg acgttggcgg acaagatccg
     3841 aatcgagagt gcgctttcgc aggctctgca ggctgcggcc gcgggaggcg ccacgggctc
     3901 tgtggcaacg ggaggcgggg ccgccgcctc gacgaccaag tcgacggtcg ggttagcgac
     3961 cgcgaaatgg ctcgcggcgc tctcggtgcc ggcgctcgtg gtcggtgcac ttcaactgcg
     4021 aaatcctgaa acaggcgagc ccagcggtat tggagcagcg agcgtggtcg ccacccgtcg
     4081 cgtggcctca aaccaggttg tggttactcc ctcggccgcg cactcgaccc cgcaaaccga
     4141 tccgggcttc gagcccgccg tggccagcca cggcgacttc gtatcgagaa atgccggctc
     4201 tcgcgctcgt tctgcctcca agccttcggc tccggattca ctgaatcagg agctggattt
     4261 gttgcacgcg gcgcagaagg catggcgaag tggtcaagcc aagcaggcct tggttctgat
     4321 cgagcgacac cagcgcgagt acccgcgctc ggtgctcggc ctcgagcgag acgccttgcg
     4381 aattctgagc ttgtgcgccg ccggtcgcga gggcgaagcc aaggcgctgg cccgaaggtt
     4441 caacggccgc cgctctccgc tcggcgctgc ggtcgagcaa agttgcgccg cggactaagc
     4501 gggggcgtag tacgctgccg aaggtgcgtc gtctccctcc gctggtgctg ctgctgtggt
     4561 ggttcgctct tccgaactcc gctcgcgccg aagagcggct acccctggag ctcgattggg
     4621 aagctccggc ggaatgctcg aacgcagact cggtgcacgc tgagctcgcg cgtatcgcgc
     4681 acgtacgcgc ggggcgggag gtgcagcctt tggcggcgaa ggtgcggatt cgaaagctcg
     4741 gcccacgtta ccgtcttttg ctgcacatca cgcagggagg gtcgagcagt gagcgcacgc
     4801 tggctgcgtc ggagtgtcgc gccttgcaac gggaggtcac gttggttttg gcgctcgcct
     4861 tcggcgaagg tgtggagcta acgaccgatg agcccgccga ggttcgagag agacatgggg
     4921 aagacgagcc gcagggaact cgaatcgatg aaccgacgcc gcccgtcgcg gctccgcctc
     4981 ccgagcctgc tcccgtgcag gtccgcactc cgaagccaac gccgccccgg cgctcaccgg
     5041 cgcccgcaaa tcgctttcgc tgggactggc ttgcgggcgg tggcgcgctc tggcgtgcgc
     5101 tgcccgttac gtcgccggtg gccttcctgg gcgcggacgt tggttttgac tcgtggtggt
     5161 tacagccgcg tgtgcagctg attcccggcg tgaatcgtga gctgccgcgc ggcattcaga
     5221 ctcgatacga tggacttgga gcaaccctcg ccggctgctc cggcgccgcg ctcgcctcgc
     5281 tgcgattggc aacgtgtctg tctctctcgg ctacggccct gcgcggccgg gcgagcggcg
     5341 ccgtagaaga agagaacgcg atcgcgccgt ggtataccgc gggtgctcac gtcagcttgc
     5401 tttggccagc ccgggggtgg cttggcgggg gcgtcgaggg tgctttcagt tactcgcttt
     5461 cgcgtccgcg ctttcgcatc aacgggttgg cggatgtgca taccgtccct cgcgtcgccc
     5521 cggcattatc cgtgtacttg cgcgtccgcc tagaaccttg aagagggctc ttggggctgc
     5581 gcttagtctc cgcatcccac gggcgccgcg gagagcacca gatcgtccat gtaaatcgcg
     5641 aacggtgtgg tctgtttgga cgaccaatcc acgcccgctc tgatcaagtg cacgccttgg
     5701 gggggcagcg tgtcgagacc ggtttgatcc agtgcgactg cgccatcgat tctgatttgc
     5761 actgcgcccg cagcttcgga caccagcacg tccgcctcga agcagaacca tcgatcgcgc
     5821 ggaacgacta gactcgtact cgtgaagcgc accggatcgt tttcggggac gaagatttcc
     5881 ggagcatcac tctcgaagtt gaagtctatg cctttgaaag gatcgggcgc gggttgatcc
     5941 cccaagaaaa gtacattggt agtcttggtc tcgactccgc tcgggattag catgaacacg
     6001 cgcaaaaagg ccgagcccga ggttacggag gaaaactcct tggccaccgc ggccgcgcta
     6061 tcgcgatcgg tactaccagc caggagcgcc ccgcgcccgc ggcgcgagac ctgctccgtt
     6121 tgagccagag aacccgcgtt caccagcact tgctcgcccc agtcggacaa atccggctgc
     6181 tcgaaccccg agcaaaccag atcttgctct cgagtggcgc agaggtcgtc tcgaaagtga
     6241 ccaatagtgt agggattgtc ctggcaccct gcgaacagca acccgaacga atacgccgcg
     6301 gctgctcgac ggaggatcat gcttccgcaa ggctagcatc ggtgttctcg gccggccgta
     6361 ggcggccgat gggacgttta agcgtcctca gtgcgcggcc tcggcgctga cgtacgtccc
     6421 ggaggaaagc gaggtcaagg gggcgggggc cgcagagagg gctttcgctt cgaaccagag
     6481 cagggtcagg acacccgcca ccgcgagtcc aaacagaagc cgggccccca tgcgccgggc
     6541 cttcacgctc cgaacatact cttcgtcgac aggtcgctcg ggttcgggat aaagcgaaga
     6601 ccagctgcgt cgagcgtagg gtgtaccgaa cgtcgattga gccatgcctg gccatgagca
     6661 atgcttgtgc cagcgacacg gcgcgggacg tgccagtgcg taaccacatt tggacgtcaa
     6721 atctgggatt caggtcgctc caaacgccac agtgaggggc tttgaacgag cactgtcgag
     6781 gtccttgttc caacgcgcct tgcccgcggt aagggatgga gtaccgaagc gaagattgcc
     6841 gaagcgatga gcattcggag cgccgctagg cgaaactctt cggtgtgggt gagattggga
     6901 tgctgcgaag gcaactcggg tgcgtgtaga gtgccccacg agcgtggcaa aaagtcgatc
     6961 tgttcaaccc cacttaccaa ctctcttccc gtcctcggga gcgccctccc gtgtggacgt
     7021 ggtggaagcg gcggagtcga gtcccgagct ggtcggcctc ggggggcagc ttccgagcac
     7081 aatccgactc gggacgatgt cgtggaatta cgcgggctgg agcggcatcg tttatagcaa
     7141 aaatgcgaac tccaaggagc tagcccggca cgggctcgcg gcgtatgcgc gccacccgct
     7201 cttgtccacc gtggagatcg accgcagcta ctacgagccg ctgccagcgc atgcttttgc
     7261 cgactttgcc aagcaggtcc cagactcctt ccgtttcgtc gtcaaggcac acgaggattg
     7321 tgtcgtacga cgttatccgc agcacgctcg ctacggcaag aagcgcggcc aagtgaactc
     7381 tctgttcttg gacgcggggt atgcgaccga gcacgtcgtc gcacccaccg tcgagggctt
     7441 gaagcaccaa ctcggggtgc tgctgttcca gtttcctccg caagacgtgg ggtccccgct
     7501 gggtttcgcc cgtcagctgg aggccttctt gcggcgctta cccgcggggg tggtctatgc
     7561 ggtagagctc cgcaatcggg agctgctcgg gcgcgcgtac gccgaggctc tagcggcgac
     7621 gggggcggtg cattgtcaca acgtgtgggg cgagatgccg gcgctgctcg agcagatcag
     7681 ggctctgccg ccggttgcgc gtcgtccgct cgtgatccgg tggttgatgc gaccgggggt
     7741 ggactatcaa tctgcggggg ctcgctactc gcctttcgac cgcctcgtgg acgaggacgt
     7801 gagccatcgc gagcaggtgg ctcgaatcgt gacgcgagcc tcggagcacg gcgtgccggc
     7861 gtttaccttg attgacaaca aggccgaagg ctgcgccccg gaaagcgcgg cgcggctggc
     7921 gcgctgtatc gtcgagaaga agcgcacggc gaccgcgcag tcggagccgc ggccggtgaa
     7981 gctggaatga gaatggcgct acgtgccgct ggctaggtct cgtccggatc gacgcggatt
     8041 acgtgcttgg cgcacgcccc gagccaattg gacccgagct cgtagctgac tctatcgccc
     8101 cgttggagag cgtcgaaaga gacatcgtcg atatcggatt cgccgaacca gatattctct
     8161 cccccggcgt ctggagcgat gaagccgaag cgcttgttct ctgcctcccg aaccaccagg
     8221 ttctggatct ttccctgctg tcgctcgctg gccttggttg tccggctcgg gcccggcgtg
     8281 ggcacgctcg gcggagcggc ggcacgtgac ggatcgcgcg tgggcaagaa caactccgct
     8341 accaagtcct tctgggtttt gtcctcgatc agattgctca ccagaattgg ataggttacc
     8401 tccgccagca acgctggtga agtttgagtc gtgcgcaaac ggccctgttg atccgtgtat
     8461 tcgaagtccc agcccagcac catcacgcgg gtgcccaacg cattcaactt gcgcgctagc
     8521 gggacgtaat ccgagtcccc ggctaccagc acgcatacgt ccagtcgctt gtagatcgcg
     8581 agttcgtacg cttctagcgc gaaccaaacg tcgatgccct tctcggcgcc tgcatagccc
     8641 atcggtaagt agtgggtcgt gacgccttca cgaaccagca cgtcttcgaa cagcctctcg
     8701 gccagcagct tgcccgcctc ggatgactct tgggcgctga atctgccgcg gaaatagtga
     8761 acgtctacga tttggcacag tcgcacgtcg acgtgctcgt gatatgcgac gcgcttcttc
     8821 acgaattcgt ggaggccgct gatgctgagc cgcgcggccc gcgcgtggtt gtaggcgtag
     8881 tagttgctga cgacgtggaa gaagttcccg tcgtagaaga tggcgattcg cgtcagggcg
     8941 tctttcatgc ggtctccgcg gagggcgatc gactgttgcc ggaggttcac gagccgtact
     9001 tcacggtgca gccgtaggcc tctgtttccg gagtgctcac ggctcggcct gcgccgagat
     9061 ccgtcagcgc cgcctccacg tagttgacga gcttgccccc gctcggagac tcaccctcgc
     9121 cgtcgggcga gttatcgatg gcgccgcggt agaccagtat cccgttctga tcgatgacga
     9181 acatatgcgg ggtgtgggcg gcgccgtaag cgcgcccgac ctgtcccgtc tcgtccaata
     9241 gaactgggtg ctgcagattg taagtcgccc gagcctcccg attgacctcg gcgcccgatc
     9301 cctgtttgcc cggagccgcc gagttgatcg cgagccacac taccccgttg gcctgctgtc
     9361 gagccgcgct gttgacgagc gaacctttgg tgtggctcgc cttcacgaac gggcagcccg
     9421 ggttgaacca ttcgatgacc acccgtttgc cccgaaaact ggagagcttg acgggctgac
     9481 cctctgtatc ggccagttcg aagtccgggg ctggagctcc aagccgcgcg gaaccaagcg
     9541 cagacggcgt gctcggagcg ctgaccggag cccgctgtgc ggcggtaggt tcagccggtt
     9601 cagctggctt gcggctgcac cccgaaccca gcgtcacgag ccaaaccacg ccccaggcgg
     9661 gcgcgcgcgt tccgcgcgct gaaaagaaag atctcatccc gcgcatcgta gggcgacggt
     9721 cgcttgaccg gaaggggcaa gtcgcctcga ttcggtcagg cttcagtcgc tggcgcggca
     9781 gtcggagccg cgctgcccta gaatagcccc tgcacttgcc aaccgagtcc agccggtccc
     9841 gtgcgtatgg tccagagtcg agcgccacga cgatcgacgg gttcgcgacc gcccgttacc
     9901 gcaaagtaga tggtcgctcc tacgcccagc gccgtgagtg cgccgcacat gtccgtcacg
     9961 agcgcggtcc gcttgagcgc accacgcgtc tcgctgatgc gggaagggga gctcggtgaa
    10021 gcctgcgagg cttgtgtata ctctttgctc gccctttgcg tgagtacgct ggatgccagc
    10081 gtcgcgcctg cggcaactcc ggtcgcgacc acggaaaccc ataaccccag gttcgtgggg
    10141 gagcccacag gggccgcact gcgcgcggcc tgatctcgcg gtaggaaaac agcccgcgtc
    10201 cgagatgggg cgtccgaatc cgtatttggt cgagcggatg cactcggttg gagagagaca
    10261 ctcagcagcc tcgattcgcc catcgagagc gtaagcactg ttcgtaccgg cttgaagccg
    10321 cgcttgcgga tgagcagacg ccgagtcccg ggattgacca gtacgaccga atcgagcgat
    10381 tcgccgcggc tctgttcgtc caccaacagt tcggcgcccg tgagatcgcc ctctagacga
    10441 acggtggcca cccgcgtctg cgcatgctcg agatcacgca tggcgcgatt tcgtagctgc
    10501 ctctgtgtct cgctttcgag cttggcgttg tctccgcctg acgtttcggc ggtagactcg
    10561 tcgagatact gtcgaagaaa ggcgatggca gcaacgtagt cgccccgcgc aaggtgcacg
    10621 ctagcgatgt cgtataggat gggaccgccg ggtatcagct cgtatgagct ctggaactcg
    10681 agtaaagcag catccaaatc cccggcgaag gccagccttc tgccgagctc ggcgtgctga
    10741 gtagcactgc gctcgttggc actgcgctcg ttggcactgc gtgccgcggt ctcgagcttt
    10801 gcagtctcga ggtctggcgt cgagtttggg ctcggttgcg cgaacgagcg cgatgcgagc
    10861 aaggtgaggc agagggttcc caacagcggc ttgaaattca tgagctctaa aacccgagtg
    10921 tcacccgagc cgtcattctc cggagtggat gagcgattcg attccgggtg tctgcctttc
    10981 gaataattcg ttacagcgca tagggatcac tattgtcgat ctcgcgcgac gggcggtcgc
    11041 tcgctctcag aacatcccca ggctggagtc gatcattctt ggacgccggc ctcgccttcg
    11101 cttcggcttc gctcggaagc gctgagttct gcggcgcgac gacgggtgcg ctcgcctgcc
    11161 gggtggccgt ggatcgcgag ttgcggcccg attctttgac cgagagccgc gggttggcgg
    11221 gggcacgcaa actcgcttgg gtagtcaagg agagcgtcag ctccagatct cgatcgaaca
    11281 gtaggtcccg ctgagacgtc tggtagcctc gctgcgacac ggtaaggacg tggctgcgcg
    11341 cgtctttccc gtgaaaggcg cggtacgggt tcgagattcg ccgcccgtcc aacctcagtt
    11401 cggcgctgct gggcttcgcc tcgatactca ccagaacctg gcccatgacc accgccccat
    11461 caacgaccgt acttcgggtg gatgaagccg cttccatcgc gggccgagcg gggaccaggg
    11521 tcaccgcgtg ggattcatga gctgctgctg aactggacga gggtgcggtt ttcgtgcctc
    11581 caccgcgaga agcgacggtt cccgcgagta tcaacaaaac tgccccgccc gcggcggcca
    11641 aagcccagcc tcgaggacgt gccgagaggc ggacgggggc ggcgctctcg agcagcggag
    11701 gcttgagtgt cgatccttct gcactcagct catgaatggg aatgggctgg ctcagcgact
    11761 cggggctcgt ggcggacaac gcagcgcgat cagccgcgag cgcgcgttga gtcttgtggg
    11821 ctcgcgagtg gcccaggtga tcctcgatgc gtcggcgcat ctcctccatt tcggagccga
    11881 actgctcgtg catgaagctg cgcaggtact cctgcccgtt ctcgaagccg atgccgcgaa
    11941 ggtagtggtc caatgcttgg ccgaactcga gcgcagactg aaacctcgcc tccggccgtt
    12001 gttctagtgc gcgattggtg atggtgatga gctcgcgcgg agcagacggt acgacctgtt
    12061 cgatgcgcaa ctcggtgcct tgtatgcgcg cttgataggc gctggcctgc gtctgcccca
    12121 taggtcgttt ttgaccggcg atggcttccc aaagcatcac tccgaccgcg tatagatccg
    12181 ttcggcggtc gaccggcttg cacaggcact gctcgggggc cgcatagccg agtttgcctt
    12241 tgatgacgcc ctcgcgggtg gcggacattg cgcccagtga cttggcgata ccaaagtcga
    12301 gcagcttgac ccccgcctcg taggtcacga acacattgga tgggctcacg tcccggtgga
    12361 cgatgctcat cggggttccg tcgaaatcca agagctcgtg tgcgtaatgt aggccagaga
    12421 ggacttgcct caatatccag aggtggagcg cgagcggcat tcgagctctg gagacttgat
    12481 tgagcaattg gtgcaggggc ttgccgtcca gaaactcgag caccagatag taatcgtcac
    12541 cctcgacaac ggcctcgtag gtttgcacca cgttcggatg gctgaggcgc accgcgattc
    12601 gcgattcatc ggcgaacatc tgaacgaact cgtcgtcgtc gaccatccca ccacgtaggt
    12661 gtttgatcac gagcagcttt cccgctgaaa atggcccggg ctggtgcgcg aggaacacgt
    12721 ccgccatgcc cccggaggtc agtttttgta cgagctcata acgcccgagg cgcatcgggc
    12781 ctcgctcgac gaggggcgac tttgcgagag cggacgactt ttcgatggca ggaagcttgg
    12841 ggtgggacat cgaagaggct ttctgttctg cggactgcag ctggctactc tgcaggtggc
    12901 tactccgcag gtggctactc cctgagttcg cggcgctatg cggatcccgg catcatgcgg
    12961 atgcctggtg ccatgcggat gcctggtgcc atgcgggttc taaagaccta cctccgagca
    13021 cgtaggagcg ctggggcggg gatctcgtag gaatagagcc agcaaccttc cgttcaggcg
    13081 gatagctgac gaacggagtt cgcgcgctct atcgcgaaac accatgaata acgttccgtg
    13141 tcatcacgca atagccccgg cgcccaggct tgggtgacgc gcaggactgc ggtgacgtac
    13201 gcaggccctc gagcgagagc ggccgtgttc gagcaagata tcgagcgagg tgtctatgtg
    13261 tgagcgacag gcgcgcggtc aca
//
LOCUS       DASTKU010000330         3051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1625868, whole genome shotgun sequence.
ACCESSION   DASTKU010000330 DASTKU010000000
VERSION     DASTKU010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625868"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VFQ61_11725"
     CDS             complement(<1..954)
                     /locus_tag="VFQ61_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9955168.1"
                     /translation="MKRRRTTVALLVDRLMSEYAVMLRRSIERAATRNDVSIVVAVGQ
                     SVRDKEPAAATQNQIYSLINQDSVDGVIIVSAILGHYCGIEGLVQMCRGYAPLPVCSL
                     GVELPGVPSIIIENQGGTRLGVEHLIDVHGSRRIALIAGPETSVESDLRIEGYRAALE
                     RRGLTVDESLIAHGTFTLPSGRDCMIELMQRGVSFDAVVSANDDMALGAMDVLRESNL
                     RVPRDLLVLGFDDINSAHFARPPLSTLRQPIWWMGESAVDSILRQLRGEAVPLVQAGA
                     VELVRRESCGCGYRATTIHQSKGSLRPSVHGIIQERRAEIAQ"
     gene            complement(1002..1370)
                     /locus_tag="VFQ61_11730"
     CDS             complement(1002..1370)
                     /locus_tag="VFQ61_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955169.1"
                     /translation="MLRVKRVALESFGIEPFVTEDRMILRLSGTGDMVAVEPLKDCLD
                     EARKDIARLSFKSLEVDIRALYLLNSSCIKALVRFIFVVQQDGPKFGIRFLVDKNLTW
                     QARALSALTRMAPDLVTIAS"
     gene            complement(1372..1875)
                     /locus_tag="VFQ61_11735"
     CDS             complement(1372..1875)
                     /locus_tag="VFQ61_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955170.1"
                     /translation="MNEPRPSATLAVTMSSFPSARIVGAVSKLVSEFCRSLIEDPDVA
                     ARFHMAAQELAENLAKYSSGPHVSISAELIGTDTSAILRLQAKNRSTPQQLEAVALRL
                     QELTSAEDPIELYDRLIRETAPLDDVSGLGLARIRAEGGLDVDYMIDGNELTISVHAS
                     VMPQGAA"
     gene            complement(2035..>3051)
                     /gene="chrA"
                     /locus_tag="VFQ61_11740"
     CDS             complement(2035..>3051)
                     /gene="chrA"
                     /locus_tag="VFQ61_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HET9955171.1"
                     /translation="GPNSTELAIHIGWARRGAAGLVIAGVSFIVPAMLMTGACGYLYV
                     RFGALPSAQWLLYGVKPVMLAVVGQAVWGLLPKAASTTALRVLGAAALVGSALAINEL
                     AVLFGSGFISVLWASTRSPARDSGSTNGQAPLGLGPLALADFTVRKAAPAAGAGLSAA
                     GLHVTAPALFWVFLKIGSVLFGSGYVLLAFLRADLVERMGWLTEGQLMDAIAVGQVTP
                     GPVFTTATFVGYVLAGPSGAALATLGIFLPAFVFVALSGPWVPRLRASRTASAFLDGV
                     NVASLALMALVTVQLGMAAVIDVPTLFLALASAILLLKYRVNSTWLVALGAFVGWCVH
                     AAGL"
BASE COUNT          641 a         1047 c          881 g          482 t
ORIGIN      
        1 ctgcgcgatc tcggcgcggc gctcctggat gatgccgtgc acgctcggtc gtagcgagcc
       61 cttggactga tggatggtgg tggctcgata accgcagcca caggactctc gacgcaccag
      121 ctcgaccgcg ccggcttgca ccagcggcac ggcctctccc cggagctgac gcaagatgct
      181 gtcgaccgcg gattccccca tccaccaaat cggctgacgc aacgtggaca agggcggccg
      241 cgcaaaatgc gccgagttga tgtcgtcgaa gccgagtacg agcaggtctc ggggtaccct
      301 caggttcgac tcgcgcagca cgtccatcgc cccgagagcc atgtcatcgt tcgcggacac
      361 caccgcgtcg aagctgacac ccctctgcat cagctcgatc atgcagtcac ggccggaggg
      421 cagcgtgaac gtgccgtgcg caatcaagct ctcgtcgacg gtcaagcctc gtcgctcgag
      481 cgccgcacga tagccctcga tgcgcaggtc ggattccacg ctcgtctcgg ggccagcgat
      541 caaggcgatg cggcgcgagc catggacgtc gatcaagtgc tccacgccga gccgagtgcc
      601 gccctgattt tcgatgatga tgctcgggac ccccggcagc tccaccccca agctacacac
      661 cggcagaggc gcgtatccgc gacacatctg gacgagcccc tcgatgccgc agtagtggcc
      721 caagatcgcg gacacgatga tgacgccgtc caccgagtct tggttgatta gcgagtaaat
      781 ctgattttgc gtcgcagcgg ccggctcctt gtcgcgcacg gattggccca ccgcaactac
      841 gatactcacg tcgttgcgcg tggccgcgcg ctcgatcgag cggcgcagca tcactgcata
      901 ctccgacatc aaccggtcga cgaggagggc taccgtggta cgccgccgct tcatggccgt
      961 tgctccaggc tcgtggattt cggcaaagct atgctccgcg ctcagctcgc aatggtgacc
     1021 aggtcaggcg ccatgcgcgt caacgccgag agagcgcgcg cctgccacgt gaggttcttg
     1081 tcgaccaaga agcgtatgcc gaactttggg ccatcctgtt gcacgacgaa gatgaagcga
     1141 accaaggcct tgatacagct cgagttcagc agatagagag ctcgaatgtc gacttcgagg
     1201 ctcttgaagc tgagccgcgc gatgtccttc cgcgcctcgt ccaaacagtc cttcagcggc
     1261 tcgaccgcga ccatgtcgcc ggtgccggac aaccgcaaga tcatgcggtc ttccgtcacg
     1321 aagggctcga tgccgaacga ctccaaagct actctcttga cccgcagcat gttaagcggc
     1381 tccctgcggc atgaccgagg catgcactga gatggtgagc tcgtttccgt cgatcatata
     1441 atccacgtcg agccccccct cggcccgaat tctcgcgagc cccaggccag aaacgtcatc
     1501 gagcggcgcg gtctcgcgaa tcaatcgatc gtaaagttcg atcggatcct cggcgctcgt
     1561 caactcctgc aggcgcaagg ccacggcctc gagctgctgg ggcgtgctcc ggttcttggc
     1621 ctgcaagcgc aagatcgcgc tggtatccgt gccgatcagc tcggccgaga tactgacgtg
     1681 cgggccagaa gagtatttgg ccaggttctc tgcgagttct tgagctgcca tgtggaagcg
     1741 ggcggcgacg tccggatcct caatcaaact gcgacagaac tccgagacca gcttcgagac
     1801 ggcgcccacg atccgggcgc tcgggaacga actcatcgtt accgccaagg tggcgctggg
     1861 gcgaggctca ttcatcggtc gggacatcgc gtcgcggtac aaccaccgcg cttttcgaca
     1921 acggctgcgc ccgacagccg gttaagcaca gttcgacgca caacgacgtg aacagcggac
     1981 ggcgccgcac acctactctg ccccacaaac aactgcagtg gccgcgccct cccttcaaag
     2041 tccggccgcg tgaacgcacc accctacgaa cgctcccagg gcaaccaacc aggtcgagtt
     2101 cacgcggtat ttgagcagca agatcgccga agcgagtgcc aggaacaggg tcggaacgtc
     2161 aatcacggct gccatcccga gttgaacggt caccaaggcc atcaaagcca gtgaggccac
     2221 gttgaccccg tccaagaacg cagacgcagt ccgtgaagct cgcaaacgag gcacccacgg
     2281 gccactcagg gccacgaaaa cgaaggcggg caaaaagatg ccgagtgtgg ccaacgccgc
     2341 accactcggc cccgccagga cataaccaac gaaggtcgcc gtggtaaata ccggacctgg
     2401 cgtcacctgc cctacggcga tggcatccat gagctgcccc tcggtcaacc agcccattcg
     2461 ctccaccaga tccgctcgca aaaacgcgag taacacgtag ccgctcccaa acagcaccga
     2521 tccgatcttc aggaataccc agaacagagc aggggccgtc acgtgaagac cagccgcact
     2581 caaccctgcg cctgccgctg gcgctgcttt gcgtacggtg aaatcggcta acgccaacgg
     2641 acccaggccg agaggagctt ggccgttcgt gctaccgcta tcccgcgccg gactgcgcgt
     2701 cgacgcccag agcaccgaaa taaagccact cccgaacagc accgccagct cgttgatggc
     2761 aagtgcagag cccaccagcg ccgccgcccc aagcacgcgc aaagccgtgg tactcgctgc
     2821 ctttggcaag agcccccaca cggcctgccc gaccaccgcc aacatcaccg gctttactcc
     2881 gtagagtagc cactgagcgg acggcaacgc gccaaaacgg acgtacagat aaccgcacgc
     2941 accggtcatc aacatggccg gcacgatgaa gctgacgccc gctataacca gccccgcggc
     3001 gcctcgccga gcccaaccga tatgaatcgc cagctccgtc gagttgggcc c
//
LOCUS       DASTKU010000331         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1403492, whole genome shotgun sequence.
ACCESSION   DASTKU010000331 DASTKU010000000
VERSION     DASTKU010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403492"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VFQ61_11745"
     CDS             complement(<1..784)
                     /locus_tag="VFQ61_11745"
                     /inference="COORDINATES: protein motif:HMM:NF025696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9955172.1"
                     /translation="MNPILDALRTLGGAASNESLDAKVMEAMRLSPTVLAIPHKPDRP
                     EYSEVAYRIGWARTYLKYAGLVTNGLRGSWTLTEQGAVTTNVDAYALASRYARSRNRT
                     ADDVDGPDSDSEHPDSAMREPEALTPLAPALRADITSRFDGLLSRDAVLRKDQAYACY
                     RRFRERFGPEVLRSLEGERLLTHMHGRHSRDSLVYWLEFKDDEDFPARFGSISGGSAL
                     KFGMYQSKQGEFWTGTGQKQVPLTLAQAIEYASRQRDELIRGA"
     gene            1125..1319
                     /locus_tag="VFQ61_11750"
     CDS             1125..1319
                     /locus_tag="VFQ61_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955173.1"
                     /translation="MPVFPDRNEAPRHGAQARVAQPLGLACGESGIVSDATLVLQVVQ
                     KRDIQKLADTLEGGEAAQAE"
     gene            complement(1750..2454)
                     /locus_tag="VFQ61_11755"
     CDS             complement(1750..2454)
                     /locus_tag="VFQ61_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9955174.1"
                     /translation="MTESANPRVALVTGSSEGIGFAIAQKLAALGITTVVTARALDRA
                     ESASRRIEGSAAIALDVTDAASVRSAFDSIAQRFGRLDILVNNAGINVDGQQLPSNTD
                     FELLHRTFETNFFGAWRVTSHAVPLMLQHGYGRVVNMSSGMGAITEAAGSEWPAYRTS
                     KTALNSLTRAFAAELEGTGILVNAACPGWVRTKLGGQGAPRSPEEGADTPVWLATISS
                     DGPSGGFFRDRAPAAW"
     gene            complement(2509..>3277)
                     /locus_tag="VFQ61_11760"
     CDS             complement(2509..>3277)
                     /locus_tag="VFQ61_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9955175.1"
                     /translation="PNEGQALWRTHMLQVHLGAPMWCRQKQAGEGTEQTFRRGQLTLV
                     PALVDAACWRRTPVSFARLQLPNALLAEVGLERMEPERMFHRFDDPVIRELTFSILER
                     AGIEGEASLCYVQSASLVIAQRIAQLTRQSRQRRFIPSAGRGLPEPALRRVLARLHEA
                     LDRNPSIEELAALAGLQAAHFSRMFRRSTGLPPHRYLQQLRLEKARALLEAGEHSVLE
                     VALAVGFSNPSHFAAFYRKHCGLSPSEQRRRAARVLR"
BASE COUNT          640 a         1043 c          972 g          622 t
ORIGIN      
        1 cggcgccgcg aatcaactcg tcgcgttggc gactcgcgta ctcgatggcc tgggccaagg
       61 tgagcggaac ctgcttctga cccgtccccg tccaaaattc gccttgcttg gattggtaca
      121 tcccgaactt cagtgcactg ccgcccgaga tgctgccgaa gcgcgcggga aagtcttcat
      181 cgtctttgaa ctccaaccaa tacactaagc tgtcgcgtga atgccggcca tgcatgtgcg
      241 tgagcaagcg ctccccttcc aggcttcgaa gaacctctgg tccgaagcgc tctcgaaacc
      301 gtcggtagca agcgtacgcc tgatctttcc gcagcaccgc atcgcgactc aacagcccgt
      361 cgaaacgcga tgtaatatct gcgcggagag cgggtgccaa aggtgttagg gcctctggct
      421 ctctcattgc cgaatcagga tgctcggagt cggaatccgg gccgtccacg tcgtctgcag
      481 tccgatttcg agatcgggcg tagcgcgatg ccagcgcgta ggcgtcaacg ttggttgtca
      541 ccgcaccttg ctccgtgagc gtccaacttc cccgcaaacc attcgtcacc agccccgcat
      601 acttcaagta cgtgcgcgcc caacctatcc gatacgcgac ttcgctgtac tcaggccgat
      661 ctggcttgtg cggtatcgcg agcaccgttg gagaaagacg catcgcctcc atcaccttag
      721 cgtctagcga ctcattgcta gcagccccgc ccaatgtccg aagcgcatcg aggatcggat
      781 tcatgtactc ctggaatacc ggaaccctaa ctgatgcctc ggttcgatcg gagccttctc
      841 caattgcgtg agacgggttc gagctcaagc cagcctcctc ttgtcgagtg gaagatgacc
      901 tcgaatcaga gcgcgcaacg cgaggccgca caagcctgat ctgatctgca aacctgccgc
      961 gttcgcgaag cgctgaccgc cgcatcccca acacgagacg gcgagatccg tcaaaccctt
     1021 acaggattgt catactaaac gactatgaga gtccaacggc tcagccgacg cgcggccgac
     1081 gtgggccact aagctgtgtt cggtaacccc ctggacactc cagtgtgcct gtgtttccgg
     1141 accgcaacga ggccccacgc cacggcgcgc aagcacgtgt cgcgcagcca ctcggcctcg
     1201 catgcggaga gtctgggatt gtcagtgacg cgacgctcgt gttgcaggta gtccagaaac
     1261 gcgatattca gaaactcgcc gacacgctcg agggcggaga agccgcgcaa gcggagtagt
     1321 aggctgttgc caatcgggat gtggggtatt ggaccgagtc gcgagcgggt cttcccctgg
     1381 cttcgcgaag tcggcggaca cttcacgatt tatggtaaag ggcgctctac tactgtgatg
     1441 gttcggattg cgaatcattg agccttgctc tcactctgag aacccggcgt gagctggcat
     1501 tctgaacgcc gctcagggtc gtggctcgcg tttgtcgccg catgtagaag accgccgccc
     1561 ccccaaagcg gcgaagcaca ccgggagctt cggaaccgcc ggtcacaccc gctagtttgt
     1621 gagaagttgg agcgcgcgga attgatctac gttctgaacc tgtgcgatcg ccgcttcttc
     1681 ctcgcaattg agaagaacgg cgattgggcg ccgtttcagc ggcccatcgt cgcaagcgcg
     1741 aagctcacgt taccaagcag ccggcgcccg atcgcggaag aatccgccgc ttgggccgtc
     1801 gctcgatatc gtcgcgagcc aaaccggggt atcggcgcct tcttcgggag agcgcggcgc
     1861 gccttgcccg cccaacttgg tgcgcaccca gccggggcac gcagcgttga cgaggatccc
     1921 cgttccttcg agttctgccg cgaacgctcg cgtcaaagag ttcagcgcgg tcttggacgt
     1981 gcgatatgct ggccattcag agccagcggc ctccgtgatc gctcccatcc cactcgacat
     2041 attcaccacg cgcccgtaac catgctggag catgagtggc acggcatgag atgtgacccg
     2101 ccaagccccg aaaaagttcg tttcgaacgt gcgatgaagc agctcgaaat ccgtgttgct
     2161 cgggagctgc tggccgtcca cgttgattcc ggcgttgttc accaagatat cgagtctccc
     2221 gaagcgctgt gcgattgagt cgaatgcact tcggacgctc gcggcgtcgg ttacgtcgag
     2281 cgcaatcgcc gccgatcctt caatgcgccg cgaggcggat tcggcacgat ccagagcccg
     2341 tgcggtaacc accgtcgtga tgccgagcgc cgcgagcttc tgcgcgatgg caaagccaat
     2401 tccctcgctg gagccggtaa ccagggccac tcgcgggttc gctgattcag tcatgcgtgc
     2461 ctacgtagtg gcgaacggga gggtttggct acccaattca tgctgtcact atcgcaaaac
     2521 tcgcgcggcg cgccgacgct gctcgctcgg cgacaagcca caatgcttgc gatagaaagc
     2581 agcaaaatgg ctcggattgg agaagcccac tgccaacgca acctcgagga cgctgtgttc
     2641 acctgcttct agcaaggccc gagccttctc cagcctcagc tgctgcaggt accgatgcgg
     2701 gggcagcccg gtggagcgcc tgaacatgcg cgagaaatgt gcggcttgta ggcccgccag
     2761 agcagccagc tcctcgatgg aggggtttcg atccaatgct tcgtgcagcc gcgcgagtac
     2821 tcgtcgcaaa gccggttcgg ggaggccgcg cccggcgctc gggatgaacc ggcgttgccg
     2881 gctctgccgc gtgagctgag caatgcgttg tgcgatgacc aagcttgccg actgcacgta
     2941 gcaaaggctg gcctcccctt cgatacctgc gcgctccaga atcgagaacg tcagctcccg
     3001 aatgaccgga tcgtcgaagc gatgaaacat acgttccggc tccatccgtt cgagcccgac
     3061 ctcagcgagc aatgcatttg ggagttgcag gcgcgcgaag gacacgggcg tcctgcgcca
     3121 gcacgctgcg tccaccagtg ccggaacgag cgtaagctgc ccacggcgaa aggtctgctc
     3181 cgtaccttcg ccagcctgct tctggcggca ccacatggga gctccgagat gaacctgcag
     3241 catatgggta cgccacagcg cctgaccctc attgggc
//
LOCUS       DASTKU010000332         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_510901, whole genome shotgun sequence.
ACCESSION   DASTKU010000332 DASTKU010000000
VERSION     DASTKU010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510901"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VFQ61_11765"
     CDS             complement(<1..139)
                     /locus_tag="VFQ61_11765"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9955176.1"
                     /translation="MGASAPSGGRRAPIVGINVTPMVDVVLVLLVIMMVSAVYIVSQS
                     LK"
     gene            complement(146..862)
                     /locus_tag="VFQ61_11770"
     CDS             complement(146..862)
                     /locus_tag="VFQ61_11770"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9955177.1"
                     /translation="MIIEKLLKVALLGASWVMYLMIALSIFSMGAMVERWWFFSKRKV
                     DADELGDKLLDSLERGDREGALKWVESSKSRSFEAEVLSPALRYLDSGAEALADAIEA
                     EWIRWRMEMERGVTLLGTLGNNAPFIGLLGTVIGVIIAFNQLGSSQAAAAMGGVMAGI
                     AEALVSTAVGLFVALPAVVAYNLIQKKIGDVESNVQVLNRKLSAFLKAQQHAHPLGHP
                     HAVHAHAHLRPVPAADAITG"
     gene            complement(859..1869)
                     /locus_tag="VFQ61_11775"
     CDS             complement(859..1869)
                     /locus_tag="VFQ61_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955178.1"
                     /translation="MMRGVALGLPLSLCLACGGQDSVIGPSEPFRVRNAQFIPGALPG
                     EPAADSAADPESPLRVSLELKNSVLLPGQGGKSLSGRAGADAWAIGVTLEGAGRGYYV
                     VPVGALDSATGELTWSATADFDESLTEGKRRLLAVAIDPSGVAGQQAARDVCIASPVH
                     PRSCSAKATPPKTVISLEWDTNADLDLQVLTPEGRLVTPKRPSTQDPEGETSAVAAGR
                     IDRDSNANCVADGIRRENLSWPTDAPLGTFGVFVNLADACKQPAVRFRVSVYTAAETA
                     PESEIEADAETEANHALVLRSEVIGELLDWQANPGSGRGMFVSEFTFPIPESPSLQGS
                     AP"
BASE COUNT          389 a          671 c          590 g          354 t
ORIGIN      
        1 ccttcaagct ctgagagacg atataaaccg ccgagaccat catgatcacg agtagaacca
       61 ggactacgtc caccatcgga gtgacgttga tcccgacgat cggtgcgcgg cgtccgccgc
      121 ttggtgcgct tgcgcccatg actcatcacc ccgtaatcgc gtccgccgcg ggaacgggtc
      181 gaaggtgggc gtgcgcgtgc acggcgtgcg gatggccgag cggatgcgcg tgctgctgcg
      241 ccttcaagaa ggcgctgagt ttgcgattga gcacttggac attgctctcc acatcgccaa
      301 tcttcttttg gattaggtta taagccacca ccgccggcag cgcgacgaac aggcccaccg
      361 cggtcgagac cagcgcttcc gcaatacccg ccatcacgcc gcccatggcg gccgcggcct
      421 ggctcgaacc gagctggttg aaggcgataa tcacgccgat cacggtcccg agcagaccga
      481 tgaacggcgc attgttgccg agcgttccaa gcagcgtgac cccgcgctcc atctccatcc
      541 gccaacggat ccattctgct tcgattgcgt cggccagcgc ctctgcgccg gaatccaggt
      601 agcgcagcgc gggtgacaac acttcggctt cgaacgagcg gctcttcgag ctctcgaccc
      661 acttcaaggc cccttcccga tctccgcgct ccaatgaatc gagcagtttg tccccgagct
      721 cgtcggcgtc gaccttgcgc ttggagaaga accaccagcg ctcgaccatg gcgcccatcg
      781 agaagatgga gagcgcaatc atgagataca tgacccagct tgcgccgagc agtgcgacct
      841 tgagtagctt ttcgataatc acggggcgct cccttgtaag gatggggact cagggatggg
      901 gaatgtgaac tcggacacga acatgccgcg gcctgaacca ggattggctt gccaatccaa
      961 caactcgcca atcacctcgc tccgcaacac gagcgcgtgg ttcgcctcgg tttcggcatc
     1021 agcttcgatt tccgactccg gagctgtctc ggccgcggta tacaccgaga cccggaagcg
     1081 caccgcgggt tgcttgcaag catcagccag gttgacgaag accccgaatg tgccgagcgg
     1141 cgcgtccgta ggccaactca gattctcacg ccgaatgcca tcggcgacac agttggcgtt
     1201 cgagtcgcga tcgatgcgcc ccgcggcaac cgcgctggtt tcgccttctg gatcttgcgt
     1261 cgaggggcgc tttggtgtca cgagccgccc ctccggagtc aacacctgca aatcgagatc
     1321 ggcattcgta tcccactcca gcgagatcac ggtcttgggg ggcgtcgcct tcgcgctaca
     1381 ggatcgcgga tggaccgggc tcgcgatgca cacgtcgcgc gcggcttgtt gcccggcaac
     1441 tcccgacgga tcgatcgcga ccgccagcaa ccgacgtttg ccctccgtaa gactctcatc
     1501 gaagtctgcc gtcgcgctcc acgtcagttc gccggtcgcg gaatcgagcg caccgacggg
     1561 aactacatag tagccgcgcc ccgcgccttc gagcgtaacc ccgatcgccc aggcgtccgc
     1621 acccgctcgc cccgatagcg atttaccgcc ttggccgggg aggagaaccg agttcttcag
     1681 ctcgagactt acccgtagtg gcgactcggg atcagccgct gagtcggcgg cgggctcgcc
     1741 cggcaacgcc ccgggaatga actgcgcatt gcgcactcga aacggctcac tcggaccgat
     1801 taccgagtct tgtccgccac aagcgaggca gagagagagc ggaagcccca acgcaacacc
     1861 gcgcatcata gattcacctg caagcgcagc gtccaggcat cgttcttcag gttggagggc
     1921 acaccttgct cgtcgcgagc caagtagtcc cgcacgaagt cgtattgaaa cgacagccgc
     1981 gcccggtccg gtagcgttaa ccca
//
LOCUS       DASTKU010000333         3768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_510904, whole genome shotgun sequence.
ACCESSION   DASTKU010000333 DASTKU010000000
VERSION     DASTKU010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510904"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VFQ61_11780"
     CDS             complement(<1..702)
                     /locus_tag="VFQ61_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9955179.1"
                     /translation="MEQLKKATRIELFSFKTPAMRAFHMSWLAFFACFFAWFAVAPLM
                     PVIKGDFGLTKGQIANINIAAVGITIIARLLIGPLCDKYGPRATYTALLALGSVPVIG
                     ISLAHSYETFLFFRLLIGAIGASFVVTQYHTSVMFAPNVVGTANAASAGWGNSGGGVT
                     QSVMPWALAALLSLGIDKSSSWRISMLVPGVLMVVVAYFYWKYTQDCPQGNYKELRAA
                     GVEVESGKKGGWAVFK"
     gene            1516..2202
                     /locus_tag="VFQ61_11785"
     CDS             1516..2202
                     /locus_tag="VFQ61_11785"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HET9955180.1"
                     /translation="MAYAISGDAWGTGAPVAPRKHALHLASLSPTQCRSELARRRIET
                     KPARSATPGVATPLRLSGSLQGVRFRTPGGNSVYGILDCRLVLALDELSRALVNVEVV
                     EVHVDNFYRPHAHLPGRKDKPSQHSYGLAADIEAFTLRDGTVLRVEQDWTPALGEPPC
                     GEEAEGTTGRGALLRKIVCDTLALGIFQHVLTPNTDAAHRNHLHFDIARSRMNARPSR
                     VKQPRPSTRG"
     gene            2486..2992
                     /locus_tag="VFQ61_11790"
     CDS             2486..2992
                     /locus_tag="VFQ61_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955181.1"
                     /translation="MKSKIFVFAVALAGGLFLEAGANATPIPGWAGMPDPMTETACWA
                     QDGARLRLAPACFDSNYHTFSIPLNPLTYGNQGLVAVSWTQNVASCGGGVCVGFGDES
                     ARAISFNPDGTISGLGSWNATAGTQLQTISYPIGGTVMLQVRMKTNSTNSHWVSSVSG
                     NGVYIDPQ"
     gene            2997..3620
                     /locus_tag="VFQ61_11795"
     CDS             2997..3620
                     /locus_tag="VFQ61_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955182.1"
                     /translation="MSSFTKWMSAVALCGAALGLVLLLREPARARPKPPVTEADSEPE
                     TPPPAALPAQPAAEVRSRPAPTPRLPASAKQAQLPAPSAHTAEAPFLQLFDADRAASS
                     ESWALRNAMMEAFQRQPRSAVTVEAMDCRFRLCRMSLLFDSVESDKTTLDAVFVKTTN
                     NDYPLGFGAVSIPERVKLPDGRVRATVYVAREGELAPPRPIADAPLE"
BASE COUNT          735 a         1206 c         1128 g          699 t
ORIGIN      
        1 cttgaagaca gcccagccgc ccttcttgcc gctttccacc tcgacgccag ccgcgcgcaa
       61 ttccttgtag ttaccctgcg gacagtcctg ggtgtacttc cagtaaaagt aggcgacgac
      121 gaccatcaac acgcctggaa ccagcatcga gatacgccag ctcgatgact tgtcgatgcc
      181 gaggctcagt agcgccgcga gcgcccacgg catcaccgat tgcgtgacac cgccgcccga
      241 gtttccccaa ccagcgctcg cggcgttggc cgtacccacc acgttcggcg cgaacatgac
      301 cgacgtgtgg tactgcgtca cgacgaagct cgcgccgatc gcgccaatca gcagcctgaa
      361 gaagaggaac gtctcgtagg agtgcgcgag agaaataccg atcacgggaa cggagccgag
      421 ggcgagcaga gcggtgtacg tcgcgcgcgg cccgtacttg tcgcacaggg ggccaatcag
      481 gagacgcgcg atgatcgtga tgccaaccgc cgcgatattg atgttggcaa tctggccctt
      541 cgtcagaccg aaatcgccct tgattaccgg catcaaaggc gcaaccgcga accaggcgaa
      601 gaagcatgca aaaaacgcga gccaactcat atggaaggcc cgcatcgccg gcgtcttgaa
      661 cgagaacagc tcgatacgcg tcgctttttt cagttgttcc accgtctacg attcctgctt
      721 caggcccgtc gattgggcat ttcacgaaag gcactcacac gagcgctcct cacctagccg
      781 gacccaagat agggactcca agtagcgagg agatttcgta gagatgaatg gacctcattt
      841 tttgcgacgt ttgtgtcgac tgaaaaaagc gcaccgaaaa catcccgaaa ctcaggctag
      901 gccgccgata gggcgctttt tctgcgtctc ctcgagattc ttggcttaca gcgcactcct
      961 cgagctagcg gcggcgacaa aaatgtctgc tttggcgcga ccgagtctct cgcgagcaac
     1021 gacgttcttc ctctgaatgc tcggtggcga gcgagctccg atggcatctc tcctgagcgc
     1081 gacattccgg caactcacct tgtgattggc aacgcgccct ccgatgcttt cctcgtcgtc
     1141 gagtttcaac agtcgaagtt gctcgacgag gttccgcaac cgcgtgcgcc tcctttgcga
     1201 tgcactcgcc tttcgtgtcc attgcggcgt cctgttcccc gcagtcgtca ctgcaaggtg
     1261 ggtgttcaaa agagagcgca ctgagtttgg ctcgtgaccg tcgacgcgaa cggacggatc
     1321 gatttcatcg tcgctcgcag actgctgttg tgagcaggcc agcgcgaagt tcatgcacgt
     1381 gacgagatga tcgagcgctg aactgcgagc ctacgagagg ttgcccgcga tgggggaggc
     1441 agctaaggtt tcgccctgac cattgctcgt cacctccagt tcttttcgaa actcgttgcc
     1501 gcctgcgcgt tcgcgttggc gtacgcgatc agcggggacg cttggggcac cggtgcgccg
     1561 gtggctccgc gaaagcatgc actgcacctg gcgagtctgt cgccgaccca atgccggagc
     1621 gaacttgcgc gccgacgcat cgagaccaag cccgcccgct ccgcgacccc cggcgttgcg
     1681 acaccgctca ggctttcggg ctcgctgcag ggcgtccgct ttcgaacacc aggcgggaac
     1741 agtgtttatg gcattctaga ttgtcgcttg gtccttgcgc tcgacgagct gtcgcgcgcg
     1801 ctcgtcaatg tcgaagtggt cgaagtgcat gtcgataact tctaccgtcc ccatgcgcac
     1861 ttacctggac gaaaggataa gccgagtcag cactcgtacg ggctagccgc cgacatcgag
     1921 gcgttcactc tgcgcgacgg aacggtgctc cgcgtggagc aggattggac gcctgcactc
     1981 ggtgagccgc cttgtggcga ggaggccgaa ggcacgacgg gccgcggtgc cttgctgcgc
     2041 aagatcgtgt gcgacaccct agcgctcggc atctttcagc atgtgctgac cccaaacacc
     2101 gacgctgcgc accgaaacca ccttcacttc gacatcgcgc gcagcaggat gaacgcgcgg
     2161 ccttcgcgcg tgaagcagcc tcgcccttcg acccgtggat gagggagccc cgggctcgat
     2221 ttcgacgacc tggggggagt ggcgcacagc gaggctctca aaatctcgcg gccggacacc
     2281 ggacaccaga gcgcgccgcg aacgtgtccg gtgctcgctc gaagccgcat cgcgctcacc
     2341 gcgggaatgt gagccagcgc cgcaatcgac cgcgtttgcc gcgcaaatcg ttaaaccaag
     2401 aaggctggac caactgctgg cagcgcgcgt tcagcgcggc acgtcgcttg ctgaaggcgc
     2461 ggctccaacc cgtgaggtaa gcaacatgaa gagtaagata ttcgtctttg cagtagcgct
     2521 ggctggtggg ctgttcctcg aagcaggcgc caacgccact cccattccag gctgggccgg
     2581 gatgcctgac cccatgacgg agaccgcctg ctgggctcaa gacggcgcca ggctgcggct
     2641 cgccccggct tgcttcgact caaattatca cacttttagc attccattga atcctctcac
     2701 ctacggcaat cagggtctcg tcgctgtcag ctggacgcag aacgtcgcca gttgtggcgg
     2761 cggcgtgtgc gtcggctttg gggacgagtc cgcgcgagcc atctcgttca atcccgacgg
     2821 cacgatatcg ggactaggta gctggaacgc caccgccggc actcaattgc agacgatcag
     2881 ttatccaatc gggggcacgg tgatgctgca ggttcgaatg aagaccaact cgaccaacag
     2941 ccactgggta tcttcagtca gcgggaacgg cgtctacatc gacccgcagt aaagccatga
     3001 gctcattcac caagtggatg agcgcggtcg ccctgtgcgg ggccgcgctc gggctcgtct
     3061 tgctactgcg ggaacccgcc cgggcgcggc caaaaccgcc cgttaccgag gcagactcgg
     3121 agcctgaaac cccaccacca gcggctctcc cggcacaacc ggccgccgag gttcgctcca
     3181 ggccagcgcc cactccacgt ctgcccgcga gcgcgaagca ggctcaactc ccagcgccga
     3241 gcgcacacac tgcagaggcg ccctttctgc agttgttcga tgcggaccga gccgccagca
     3301 gcgagtcctg ggcgttgcgc aacgcgatga tggaggcttt tcaacggcag ccgcgctcgg
     3361 cagtgacggt cgaagcgatg gactgtcgct ttcgcttgtg tcggatgagc ctattgttcg
     3421 atagcgtcga gtccgataag accacgctcg acgccgtctt cgtaaaaact acgaacaacg
     3481 actaccctct gggcttcggg gccgtcagca ttccagagcg tgtcaagtta cctgacggcc
     3541 gcgtgagagc caccgtctac gtcgctcgcg agggtgagct cgctccgccg cgcccgatcg
     3601 ccgacgcgcc attggagtga tgtagagcgg gtttaggtcc cgctcgcgag tacccgacgt
     3661 gtgcccgcta tacccgatct tcgttcgcga acgcagggtg ccgccctcga aacaaaaagg
     3721 aaatcgcgtc cactggacac gctgaagacc tcctggcgtg tccggtgt
//
LOCUS       DASTKU010000334        11592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_510925, whole genome shotgun sequence.
ACCESSION   DASTKU010000334 DASTKU010000000
VERSION     DASTKU010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510925"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..2074)
                     /locus_tag="VFQ61_11800"
     CDS             complement(146..2074)
                     /locus_tag="VFQ61_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HET9955183.1"
                     /translation="MTQPVNTPVHESKRKLPLVAAQAATAAKGSLTDVDAALKAFEAE
                     ERKRLGLPEEEHWIEDMANLSFSKREKGKITLLIGGLTMAHDFLVEGAFKSLGYNVVA
                     LDCPDYEALQVGKEFGNRAQCNPTYFTVGNLVKCLIHLRDKKGLSARQIIDEYVFLTA
                     GACGPCRFGMYVTEYRKALRDAGFDGFRVMLFQQQGGLSQATGEESGLELNPKFFLSL
                     LKALLAGDIINGIGYRLRPYETVPGSTDAAMKRAKQICYDALHDRRSILLALMRAKRE
                     FAKVEVDRAQLKPKVAIIGEFWAMTTEGDGNYQLQRFVEKEGAEADIQFVSAWILYNI
                     WEVAFDTKSRELLRQHDGGRSGLAGSSEFAIFQREVGLWAADKALRAAFQTFAYAGGF
                     YGYHLPDMGEVADIAAPYYNNDLRGGEGHMEVGKLILNVVKNKATMTLSVKPFGCMPS
                     SGVSDGVQSLISERYPGTIFCAVETSGDGAVNFQSRVQMYLFKAKQAAQAEFEKALAE
                     TGLDLEGFRALLKKHPRYASALHHPPHRGGTTTTDLLFEVAELAGLTPVQRVQRSLTN
                     AAKATGALVTEGVRQAPQSARAIGKFSSRAARELFGIAKEKAPVVAEQAREKLGRVLP
                     FTRAAARDAETTVPVAAE"
     gene            complement(2142..5786)
                     /locus_tag="VFQ61_11805"
     CDS             complement(2142..5786)
                     /locus_tag="VFQ61_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HET9955184.1"
                     /translation="MSDLVIGMDVGSTTVKAVVIDPETRAILWHDYQRHHTKQPEKVL
                     EFLRRILAAFPDHSSESFRLFLTGSGSGPLAPSTGGKFVQEVNAVTLAVEDRHPDVLS
                     VIELGGQDAKIIMFKEDKNTGQKTAGTSMNDKCASGTGATIDKCMIKVGAEPGFATSL
                     HFNDAKLHHVAAKCGVFAETDIVNLVKTGIPKDEVLNSLADAIVLQNLSVLTRGNTLK
                     PKVLLLGGPNTYLPFLRECWQQRIPETWRDRGFDYPKDVPIEELIFVPPNADLYAAYG
                     AAMYGILEGGEAGKFAGVESLEDFIANGRRARLGEQAGPPLSSTADETRSFVESYRIP
                     RFEPPALRVGQVVRGVIGVDGGSTSSKAVVIDESGEIVCKAYQLSKGNPIQDTKDLLA
                     KIQDHITSQGAVLECIGFGATGYAADVLEQTVLADVNIVETVAHMMSAVHFFGDIDVI
                     CDIGGQDIKVLFMKNGDIQNFKLSNSCSAGNGMLLQAMADQFGIPVTEYADQAFKAEL
                     APKFSYGCAVFLDSDRVNFQKEGYQKEELLAGLAQVLPKNVWQYVVQIPRLASLGRKF
                     VLQGGTQYNLAAVKAQVDYIKERVPDAEVFVHPHTGEAGAIGAAIETLRRYRRSRTTR
                     FIGVEATLALDYTTKNDESTVCHFCPNECKRTFIDTARPDGSTARYISGFSCEKGTVE
                     SKDAMLALVAERKKTAAEYPNLVDYESKRAFMHFYTPEPLPAAGSAVRDVRVVKGLFR
                     VKREEYSRPFVRSSAEAEKRRKSIRVGMPRVLNMYSTAPFFRTYFESLGLGRSNIVFS
                     EHTSEEMWVEGGKYGSVDPCFPSKVVQAHVHNLLFHKHEPERNRALDYVFFPILTHVA
                     NFVVDAMDNASCPIVAGTPDVVKAAFTKEVDFFAQRGIEYLDPALSFMEPNLLARRMF
                     ETWGARLGITEDENDHACKQGWLALQEFERDLQEKGRAILETVEAEDRIAILVLNRPY
                     HADPGLNHGIPEEFQVLGYPILSVRSIPKDREYLDRYFKDELEKGIIKSPLELNHIWP
                     ENYSVNSTQKVWGANFAAHHPNVAVLDLSSFKCGHDAPTYGMIDSVLQTSKTPAAALH
                     DIDANKPGGSIKIRVKTYAHSLRLQQERLEDVARKRRELAVNLDRKRLELLQLRHAQL
                     EAIMRRDADLDRQIAELSERVRSYEAQISAPRDVPEGVVQLGRKREDGSIQRLSSKVS
                     RETVAVPAAE"
     gene            6267..6941
                     /locus_tag="VFQ61_11810"
     CDS             6267..6941
                     /locus_tag="VFQ61_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9955185.1"
                     /translation="MSRSPRARAELAKAERREQILKSAREVFARRGYHATTIDDIVAD
                     AGLARGTFYLYFDDKRAIFSELIERFAARITQAIQRIDPNDPERSVDLQARDNIRGIL
                     RTCLAERTMTKLLLTDVAGADPNFDQKLRAFYETVSQLLAESLKDGQALGIVAHGEPR
                     VLACLTIGALKELLYQAVTLGFAEETADVLTQQMYGFLSRGYLRVDTPKSKPKPERAR
                     RNRRRT"
     gene            7187..10099
                     /locus_tag="VFQ61_11815"
     CDS             7187..10099
                     /locus_tag="VFQ61_11815"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET9955186.1"
                     /translation="MSHDHSESTPNTLSLSFLEGLYAEFIKDPSSVPAEYREYFANIE
                     PTEDFARTPRLGPAFRPASLFNPPAPVAAAPAPRAVARVNGNGHAAAAADQLHEDAIH
                     AAPAHATRGQTLSGVSDAAIRQDRLDALIRAYRVRGHMIAKIDPLGLPRPSQPELDPE
                     FYDLYADDLERKFSSRTIFGAETLTLREILNRLRNTYCRSIGVQFMHIDDLAVKNWLQ
                     DRMEGSENRIQLSRGEQLRILTKLTDAVIFEEFIQKKFVGAKSFSLEGSESLIPLLAF
                     AIDRAAEHEFDEIVLGMAHRGRLNVLANIMGKSPRDIFREFQDSDPELFFGSGDVKYH
                     LGHSSDFIASNGGKVHLSLCFNPSHLEYVNPVVMGRARAKQDRVGDVARSKKLAIMIH
                     GDAAFAGEGIVQETLNLSQLDAYTVGGTIHVIVNNQIGFTTPPQQSRSTTYATDVAKM
                     LQIPIFHVNGEDPEAVAQVVRLALDFRDTFLRDVVIDMYGYRRHGHNETDEPAFTQPL
                     LYRAIQARKSVRDGYLEHLLRLNGVTREEADRITIERREHLEAELNEASRKDFVRLQD
                     WLGGYWKGYRGGPEADVADVDTGLDRARASQLLLKQTELPESFRPHKQIEKLLGQRRA
                     MANGERPLDWGTAELLAFGSLVTEGTRVRLTGQDSGRGTFSSRHAVLHDFENGHTHIP
                     LCHLDPKQAPIEIYNSALSEAGVLGFEYGYSLDWPDALVLWEAQFGDFANAAQVIIDQ
                     FIVSAEDKWKRLSGLVLLLPHGYEGQGPEHSSARIERFLTLASEENIQVAQPTTPAQY
                     FHLLRRQVLRPWRKPLVVFTPKSLLRHPQCASPLDEFTSGHFQRVISDSSVKKASRVL
                     LCSGKVYYDLEKRRTELGRHDVALVRMEQYYPLPDVYLRAAIDQYSDGTPVVWVQEEP
                     ENMGAWRLLRARFGPVLFGRFPFSGVCRAESASPATGSASCHKLEQQELLERAFAG"
     gene            10135..11421
                     /gene="odhB"
                     /locus_tag="VFQ61_11820"
     CDS             10135..11421
                     /gene="odhB"
                     /locus_tag="VFQ61_11820"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF004309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HET9955187.1"
                     /translation="MSIELKVPPVGESITEVQIGEWLKSEGDLVKRDEVIVKIETDKV
                     TVDLPAPVSGKLLQIKVKPGQAAAVGDVIGYLEEGAAAEAPKSAPKAEPAAKAEPAPK
                     AEPAPAPKPAQPEATTGSRDVRDTLPPQAFARVMPSARRALKERGLRAEDVEATGPGG
                     RILKEDVMRAKGPEGRSEHAPEHAARPAAPAGQQIERAEDVVAMSPFRKRIAERLVES
                     QNTAALLTTFNEIDMSGVMALRKEYQEPFQERYGIKLGFMSFFVKAAIDALKLVPAVN
                     AEIRGKDIVYKNYYDIGVAVGGGKGLAVPVIRNAERLSFAEIEQSIADFGKRAKDNKL
                     ELSELLGGTFTISNGGIYGSLLSTPIINPPQSGILGLHAIQDRPVAVNGQVVIRPMMY
                     IALTYDHRLVDGREAVTFLKRIKQCLEQPARMLVEV"
BASE COUNT         2215 a         3692 c         3500 g         2185 t
ORIGIN      
        1 tgagcgatac ggcagagccc ctattggtcg actcgttcga gtggtcaccc aagcgcggca
       61 cgtaggtagt ccaacgccaa gcgagcgctt agacgcgccc tcgaggggcg gccccagcgc
      121 tcactcggca ttcaacgact tcttctcact cggccgcgac gggaacggtg gtttcggcat
      181 cccgcgccgc ggcgcgggtg aacggcagca cgcgccccaa cttctcgcga gcttgttcgg
      241 cgaccaccgg cgccttttct ttggcgattc cgaacagctc tcgcgccgcg cgcgaagaaa
      301 acttaccaat cgcccgcgcc gactgcggcg cctgtcggac gccttcggtc accagcgcgc
      361 cggttgcttt cgccgcgttc gtgaggctgc gctgtacgcg ctgcacgggc gtgagcccag
      421 cgagctccgc gacctcgaac aacagatcgg tcgtcgtggt tccgcctcgg tgcggcggat
      481 gatgcagcgc cgacgcgtac cgaggatgct tcttcagcaa cgcgcggaag ccctccaaat
      541 cgagccccgt ctccgcgagc gccttctcga actcggcttg agccgcctgt ttggccttga
      601 acaagtacat ttgtacacgg ctctggaagt tgaccgcgcc gtcaccactc gtctccaccg
      661 cacagaagat cgtgccgggg tagcgttcgg aaatcagcga ctgaactccg tcgctcacgc
      721 ctgagctcgg catacagccg aacggcttga cgctcaaggt catggtggcc ttgttcttca
      781 ccacgttgag gatcagctta ccgacctcca tgtggccttc accgccgcgc agatcattgt
      841 tgtagtaggg cgcggcgatg tcggcgacct cgcccatgtc cggcaggtga tagccataga
      901 agccgcccgc ataagcgaac gtctggaagg ccgcgcgcag cgccttgtcg gccgcccaca
      961 ggcccacctc gcgttggaag atcgcgaact cgctgctgcc agcgaggccc gaccggccgc
     1021 catcgtgctg acgtagcaac tcgcgcgact tggtatcgaa cgctacttcc cagatgttgt
     1081 agagaatcca cgctgacacg aactgaatat cggcctctgc gccttccttc tcgacgaagc
     1141 gctgaagctg gtaattgcca tcgccctccg tggtcatggc ccagaactca ccgatgatgg
     1201 ccaccttggg cttgagctgc gcgcggtcta cttcgacctt ggcgaactcc cgcttcgcgc
     1261 gcatcaacgc gagcaagatg gaacgtcggt cgtggagcgc gtcatagcaa atttgcttcg
     1321 cgcgcttcat cgccgcgtcg gtcgagccgg gcacggtctc gtagggccgc aaacgatacc
     1381 caatgccgtt gatgatatca ccggccaaca gtgccttgag caaggacaag aagaacttcg
     1441 ggttcagctc gaggccggat tcttccccgg ttgcctggct caggccgccc tgctgctgga
     1501 acaacatcac gcggaaaccg tcgaagcccg cgtcgcgcag cgccttgcga tactcggtca
     1561 cgtacattcc gaagcggcac gggccgcacg cgcccgccgt taggaacacg tactcgtcga
     1621 tgatctgccg cgcgctcagg cccttcttgt cgcgtaggtg aatcaagcac ttcacgaggt
     1681 taccgacggt aaaatacgtc ggattgcact gcgcacgatt gccgaattcc ttgccgacct
     1741 gcagcgcctc gtagtcggga caatccagcg ccacgacgtt gtaaccgagg ctcttgaaag
     1801 cgccctcgac gaggaagtcg tgagccatcg tcaaaccgcc gatcagcagc gtgatcttgc
     1861 ccttctcacg cttcgagaag gacagattgg ccatgtcctc gatccagtgc tcctcttccg
     1921 gcaggcccaa acgcttgcgc tcctccgcct cgaaggcttt gagcgccgcg tcgacgtcgg
     1981 tcagcgatcc cttcgccgcc gtcgcggcct gcgccgcgac gaggggaagc ttcctcttcg
     2041 actcatgaac tggtgtgttt acgggttgtg tcatggcttc ctcctcgtgt gaggtattct
     2101 gaacgcacaa tcgcgcagcg aggcccgtgc gcggcctgct gtcattcggc tgccggaacg
     2161 gccacggtct cgcggctcac cttggaactc agacgttgaa tgctgccgtc ttcgcgcttc
     2221 ctaccgagct ggaccacgcc ttccggcaca tcgcggggag cgctgatctg cgcctcgtag
     2281 ctgcgcaccc gctccgaaag ctcggcaatc tggcgatcca aatcagcgtc tcgacgcatg
     2341 atcgcctcga gctgagcgtg ccggagctgc aacaactcga gacgcttgcg gtccagattg
     2401 acggcgagct ctcgccgttt ccgagccacg tcctccagcc gctcttgctg cagacgcaac
     2461 gaatgagcgt aagttttgac tcggatcttg atcgagccgc ccggcttgtt tgcgtcgata
     2521 tcgtgcagcg cggcggccgg ggtcttggac gtctgcagca cgctatcgat catgccgtag
     2581 gtgggcgcgt catggccgca cttgaagctc gagaggtcga gcaccgcaac attgggatga
     2641 tgagccgcga agttcgcgcc ccataccttc tgagtgctgt tgaccgagta gttctccggc
     2701 caaatatggt tcagctcgag cggagacttg atgatgccct tctcgagctc gtctttgaaa
     2761 tatcgatcta ggtattcacg atcctttgga atcgaacgaa cgctcaagat cggatatccg
     2821 agcacctgga actcttcggg aatcccgtga ttgagccctg gatccgcgtg gtacggccga
     2881 ttcaggacca agatcgcgat ccgatcttcg gcctccaccg tctccaagat cgcgcgccct
     2941 ttctcttgca agtcacgctc gaactcctgc agcgcgagcc agccttgttt gcaggcgtgg
     3001 tcgttctcgt cctcggtgat accgagccgg gcgccccacg tctcgaacat gcgacgggcg
     3061 agcaggttcg gctccatgaa cgacagcgcc gggtcgaggt attcgatgcc ccgttgggcg
     3121 aagaaatcga cttctttggt gaaggctgcc ttcaccacgt cgggcgtgcc ggcgacgatt
     3181 gggcacgagg cgttgtccat cgcgtccacc acgaagttgg caacgtgggt gaggatcggg
     3241 aagaagacgt agtcgagcgc gcgattccgc tctggctcgt gcttgtggaa cagcaagttg
     3301 tgaacgtggg cctgcaccac cttgctgggg aaacacgggt cgacgctgcc gtacttgccg
     3361 ccttcgaccc acatctcctc ggacgtgtgc tcgctaaata cgatattgga gcgaccgagc
     3421 ccgagcgact cgaagtacgt gcggaagaac ggcgccgtgc tgtacatatt gagcacgcgc
     3481 ggcatgccaa cacggatgct cttacgacgc ttctcggcct ccgcgctcga tcgcacgaac
     3541 ggccgcgagt actcctcgcg tttgacccga aacagccctt tcacgacgcg cacgtcacgc
     3601 accgccgagc ccgccgcggg cagcggctcc ggcgtgtaga agtgcatgaa cgcacgcttc
     3661 gattcgtagt cgaccagatt gggatattcg gcggccgttt tcttacgctc ggcgaccagc
     3721 gcgagcatgg cgtccttgct ctcgaccgtg cccttctcgc aggagaaacc ggagatgtag
     3781 cgcgcggtcg atccgtccgg gcgcgccgta tcgataaaag tgcgtttgca ctcgttcgga
     3841 cagaagtggc aaacggtcga ttcgtcgttc ttggtcgtgt agtcgagcgc gagcgtcgcc
     3901 tccacaccga tgaagcgcgt cgtgcgcgag cgacgatagc gccgtaacgt ctcgatcgcg
     3961 gccccgatcg cgcccgcttc gccggtgtgc gggtgcacga acacctccgc gtcgggcacg
     4021 cgctccttga tatagtcgac ctgcgctttg accgcggcca ggttgtactg cgttccccct
     4081 tgcaatacga acttgcgccc gagcgaagcc aagcgaggaa tctgcacgac gtactgccag
     4141 acgttctttg gcagcacttg cgcgaggccc gccagcagct cctctttctg gtagccctcc
     4201 ttttggaagt tcacacgatc ggagtcgaga aacaccgcgc agccgtagct gaatttcggg
     4261 gccaattcgg ccttgaatgc ttgatcggcg tactccgtga cggggatacc gaattgatcc
     4321 gccatcgcct ggagcagcat gccgttgcca gcgctgcacg agttggacag cttgaaattc
     4381 tgaatatcgc cgttcttcat gaacaggact ttgatgtcct gcccgccgat gtcgcagatc
     4441 acgtcgatgt cgccgaagaa gtggacggcg ctcatcatgt gagccacggt ctcgacgatg
     4501 ttcacgtccg cgagcaccgt ctgttcgagc acgtccgcgg cgtagccagt cgcgccgaag
     4561 ccgatacact ccaacaccgc gccttggctc gtgatgtggt cctggatttt ggccagcaga
     4621 tccttggtgt cctggatcgg gttgcccttg gagagctgat acgctttgca gacgatttcg
     4681 ccgctctcgt cgatcaccac ggccttggag gaggtcgagc caccatccac gccgatcaca
     4741 ccgcgcacca cttgccccac ccgtagcgcg ggcggctcga agcgcgggat gcggtagctc
     4801 tccacgaagg agcgcgtctc gtctgccgtc gatgacagcg gcggccccgc ttgttcaccc
     4861 aagcgggcgc ggcgaccgtt ggcgatgaag tcttccaaag actccacgcc ggcaaacttc
     4921 ccggcctcgc cgccctcgag gatgccgtac atcgccgcgc cataagcggc gtagaggtcg
     4981 gcattcggcg gcacgaaaat tagctcctcg atcggaacgt ccttgggata atcgaagccg
     5041 cgatcgcgcc aggtctctgg gatgcgctgc tgccagcatt cgcgcaggaa gggcagatac
     5101 gtgttgggcc cgcccagcaa cagcaccttg ggcttcagcg tattgccgcg cgtgagcacg
     5161 ctcaggtttt gaagcacgat cgcgtccgcc agggagttca ggacctcgtc cttggggatg
     5221 cccgtcttca ccaggttcac gatgtccgtc tcggcgaaca caccgcattt ggccgcaacg
     5281 tggtgcagct tcgcatcgtt gaagtgcaag ctcgtggcga agcctggctc ggcgcccacc
     5341 ttgatcatgc acttgtcgat ggtggccccg gtgccggacg cacacttgtc gttcatggag
     5401 gtccccgccg tcttttggcc ggtgttcttg tcctccttga acatgatgat cttggcgtcc
     5461 tgaccaccga gctcgatcac gctgagaacg tcgggatggc gatcttcaac ggccagggtg
     5521 accgcgttca cctcctgaac gaacttgccg ccggtcgagg gggctagagg gccggagccc
     5581 gatcccgtca agaacaaccg aaaggactcc gaagagtgat cgggaaacgc cgccaggata
     5641 cggcgcaaga actcgagcac cttctcgggt tgcttggtgt gatgccgctg atagtcgtgc
     5701 cagagaatgg cccgagtctc gggatcgatc acgaccgcct ttacggtggt cgacccgacg
     5761 tccatgccta tcacgaggtc gctcatcaca tgccctttct acgagctgac gtgctttcca
     5821 gatccgcggc gctgctcttg tccgaactgt cttgtctgaa cgtttgctga acgtgccgtc
     5881 tgtgccgtac gcgccgattg tcgaacgagc catcccaaga atgacgctga cgtcagtgtt
     5941 tagtccggaa cacccgcctc tgacaagcaa acccctaccc gcgcttacgt ttcggaacgt
     6001 cgctgcttgc aaacccggga aacttcgagc caaagacgcg ggagggcctg actacatcgg
     6061 cgccacaacg agcggctctg ttggggcccc gcctacgcgt ggaacgcccg gcccggcaac
     6121 tgaccgcggc cgctcggcgc tccattcccc gcgacttgcg gtcggcccga acccgcgtcg
     6181 ccttcacgga ctcgaggcgc cccccgcgct ccccacgctt cacgagccaa gtcgcgcgga
     6241 gctggttaag atgcggcccc ttgtcaatga gtcgtagtcc gcgagcacgc gccgagctgg
     6301 ccaaggccga aaggcgtgaa cagattctga aatcggcgcg tgaggtgttt gcccggcgcg
     6361 gatatcacgc caccacgatc gacgatatcg tggcggacgc cgggctcgcg cgcggcacgt
     6421 tctatttgta cttcgatgac aagcgcgcca ttttttcgga gctcatagag cgcttcgcgg
     6481 cgcgcatcac ccaagcgatt cagcgcatcg acccgaacga tcccgagcgc tcggtagact
     6541 tgcaagcccg cgacaacatc cgcggcatac tgcgcacgtg tttggcggag cggacgatga
     6601 ccaagctgct gctcacggat gtggccgggg ccgatcccaa cttcgatcaa aagctgcggg
     6661 cattctacga gacagtcagt caactcctcg ccgagagcct caaggatggg caagcgctcg
     6721 gtatcgtcgc gcatggtgag ccacgggtgc tcgcttgcct caccatcggt gcactgaaag
     6781 agctcctcta tcaagccgta acgctcggtt tcgcggagga gaccgcagac gttctcacgc
     6841 agcagatgta tggattcctg agtcgaggtt acttgcgcgt cgacacaccg aaatcgaagc
     6901 ccaagcccga gcgcgcccga cgcaaccggc gtcgaacttg aacctacgag cgttacagat
     6961 acggattccg aagcgccaca atcggcgaca cgcacctcgg aatttacaag aactgcggca
     7021 aatttaaacg ctgaatgttt ccgcttcatt gcaagagcgc cggccgccgg acccgactca
     7081 cttattgggt caaagcgtcg caaacgaaag cttgacccgt ttcgcgaccg ggcccattcc
     7141 atgcgtcgct tccgacccga cgcgcacgag gttttccaaa cccccaatgt cacacgatca
     7201 tagcgaaagt actccgaata ccctgagcct ctcgttcctc gaagggctct acgccgaatt
     7261 catcaaagac ccgagcagtg ttccagcaga gtatcgggag tacttcgcca acatcgagcc
     7321 caccgaagac tttgcgcgga cgccgcggct gggtccggcg ttccgccccg ctagtttgtt
     7381 caatcctcct gccccggttg cagccgcgcc tgcgccgagg gccgtcgctc gggtaaacgg
     7441 aaacggacac gccgcggcgg cggctgacca actgcacgag gatgcgattc acgccgcgcc
     7501 cgctcacgcc acgcgtgggc agacgctgag cggcgtatcg gatgccgcaa tccgacagga
     7561 tcggttggac gccctgattc gcgcctaccg cgtgcgtggg cacatgatcg ccaagatcga
     7621 tccgctgggc ttgccgcgcc cgagtcagcc cgagctcgat ccggagtttt acgacctata
     7681 cgccgacgat ttggagcgga agttctcgag ccgcaccatc ttcggcgccg agacgctcac
     7741 gctgcgcgag attttgaatc gtctgcgcaa cacttattgc cgctcgattg gcgtgcagtt
     7801 catgcacatc gacgatctgg cggtgaagaa ctggctccaa gatcggatgg aaggctccga
     7861 gaatcggatt cagctgagcc gaggcgagca gctccgcatt ctcaccaagc tgaccgacgc
     7921 ggtgatcttc gaggagttca tccagaagaa gttcgtgggc gcgaagagct tctcgctcga
     7981 gggctccgag agcttgatcc cgctgctggc cttcgccatc gatcgcgcgg cagagcacga
     8041 gttcgacgag atcgtgctgg gtatggctca tcgcggccgt ttgaatgtgc tcgctaacat
     8101 catgggcaag agcccgcgcg acatcttccg tgagttccaa gactcggatc cggagttgtt
     8161 tttcggcagc ggcgacgtga aatatcacct cgggcacagc tcggatttca tcgcatcgaa
     8221 cggcggcaaa gtgcacctct cgttgtgctt caacccgagc cacctcgagt acgtgaatcc
     8281 ggtggtgatg ggccgcgccc gcgccaaaca agaccgagtc ggcgacgtgg cgcgcagcaa
     8341 gaagctcgcg atcatgatcc acggggacgc ggccttcgca ggcgagggca tcgtccaaga
     8401 gacgctgaat ctgagccaac tcgacgcgta cacggtgggc ggcacgattc acgtcatcgt
     8461 aaacaaccaa atcggattca cgaccccacc gcaacaaagc cgctcgacga cttacgcgac
     8521 cgacgtcgcg aagatgctgc aaattcctat cttccacgtg aacggcgaag atccggaagc
     8581 ggtggcccag gtggtgcgct tggcgctgga tttccgcgac accttcctgc gcgatgtcgt
     8641 gatcgacatg tacggctatc gccggcacgg ccacaacgag acggacgagc cggcgttcac
     8701 gcagccgttg ctgtatcgcg cgattcaggc acgcaagagc gtgcgcgacg gctatctgga
     8761 gcacttgctg cgcttgaacg gcgtgactcg agaggaagcg gaccgcatca ccatcgagcg
     8821 ccgcgagcac ctggaagcgg aactcaacga ggctagccgc aaggatttcg tcaggttgca
     8881 ggactggctc ggcggctatt ggaagggcta ccggggtggc cccgaagccg acgtggccga
     8941 cgtcgatacg ggcttggatc gtgcgcgagc gagtcagttg ctactaaaac agaccgaact
     9001 tcccgagagt ttccgtccgc acaagcagat cgaaaagttg ctcgggcagc gccgggcgat
     9061 ggccaacggg gagcgcccac tcgactgggg aaccgccgag ttgctggcct ttggatcact
     9121 cgtcaccgaa ggcacccgcg tgcgcttgac gggtcaggat tcgggccgtg gcacgttcag
     9181 ctcgcggcac gcggtgctac acgatttcga gaacgggcat acccacatcc cgctctgcca
     9241 cctcgatccg aagcaagcac caatcgagat ctacaactcg gctttgagtg aagccggcgt
     9301 gctcggcttc gaatacggct acagcctgga ttggccggat gcgctcgtgt tgtgggaagc
     9361 gcagttcggc gacttcgcca acgccgcgca ggtcatcatc gatcagttca tcgtcagcgc
     9421 cgaggacaag tggaagcgac tgtccggcct cgtgctcttg ttaccccacg gttacgaggg
     9481 ccagggtccg gagcactcga gcgctcgcat cgaacgtttc ctgacgttgg cgtccgaaga
     9541 gaacatccag gtcgcccagc ccacgactcc ggcccagtac ttccatctgt tgcgccgcca
     9601 ggtgctgcgc ccctggcgca agcctctggt cgtcttcacg cccaagagcc tgctgcgcca
     9661 cccgcaatgt gcgagcccgc tcgacgagtt cacgagcggt cacttccagc gcgtgatcag
     9721 cgattcgagc gtcaagaagg caagtcgagt gctgctgtgc agcggcaagg tctactacga
     9781 cttggagaag cgtcgcacgg agctggggcg gcacgacgtc gcgctggtgc gcatggagca
     9841 gtactacccg ttgcccgacg tttacctgcg tgccgccatc gaccagtact ccgacggtac
     9901 gccggtggtc tgggtccagg aagagccgga gaacatgggt gcctggcgcc tactgcgcgc
     9961 ccgcttcggt cccgtgttgt tcgggcgctt cccgttcagc ggcgtgtgcc gcgctgaatc
    10021 tgcaagtccg gcgactggat ctgcgagctg tcacaagctc gaacaacaag agttgctcga
    10081 gcgtgccttc gccggatgac ttcgtcacga gcttgaacta aggaattggt tggcatgtcc
    10141 atcgagctga aagtccctcc cgtgggcgag tccattaccg aggtacaaat cggtgagtgg
    10201 ctcaaatccg aaggtgatct cgtgaaacga gacgaggtca tcgtgaagat cgaaaccgac
    10261 aaggttacgg tcgatctccc tgcgccggtc agcggcaagt tgctgcaaat caaggtcaag
    10321 ccgggtcaag cggcggccgt cggtgatgtg atcgggtact tggaggaggg ggccgccgct
    10381 gaagccccca aatctgcgcc caaggccgag ccagcggcga aggcagagcc cgcgcccaaa
    10441 gcggagcccg cacccgcccc gaagcccgct caaccggagg cgaccacggg atctcgagat
    10501 gtacgcgata ccttgccgcc gcaagccttc gctcgagtca tgccgtcggc tcgtcgcgcg
    10561 ctgaaagaac gcggcttgcg cgcggaggac gtggaagcca cgggccccgg agggcgcatc
    10621 ttgaaagaag acgtgatgcg agccaagggc cccgaaggcc gctcggagca tgcccccgag
    10681 cacgccgcgc gccccgcggc gccagccgga cagcagatcg agcgcgccga ggacgtggtg
    10741 gcgatgagcc cattccgcaa gcgaatcgcg gaacgtctgg tggagtcgca gaacacggcg
    10801 gcgctgctca cgaccttcaa cgaaatcgac atgtccggcg tgatggcgct gcgcaaggaa
    10861 tatcaagagc cgttccagga gcgctacggc atcaaacttg gtttcatgtc cttcttcgtg
    10921 aaggccgcga tcgacgcgct caagctggtg cccgccgtga acgcggaaat ccgcggcaaa
    10981 gacatcgttt acaagaacta ctacgatatc ggggtcgcgg tcggtggagg caaggggctc
    11041 gccgtgcccg tgattcgcaa cgccgagcgc ctcagcttcg ccgaaatcga gcaatcaatc
    11101 gcggatttcg ggaagcgcgc caaggacaac aagctggagc tttcagagct gctcggcggc
    11161 accttcacga tctcgaacgg cggaatctac ggttcactcc tctccacacc aatcatcaac
    11221 ccgcctcaaa gcggcatcct gggtttgcat gccattcaag accggcctgt cgccgtcaat
    11281 ggccaggtcg tgattcgacc catgatgtac atcgcattga cctatgatca tcgcctggtc
    11341 gacgggcgcg aagccgttac cttcttgaag cgcatcaagc aatgtctgga gcagccagca
    11401 cgcatgctgg tcgaagtgtg aagcgcttca gcgcgttgaa tctacggaag gaacacggat
    11461 gatccaccac gatctgatcg tgatcggcgc cggtccggct ggctatgtcg cggccatccg
    11521 agcagcccaa ttgggcttga acgtggcttg tgtggagcag gaacccgagc tcggaggcac
    11581 ttgcctacga gt
//
LOCUS       DASTKU010000335         3900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_64994,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000335 DASTKU010000000
VERSION     DASTKU010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64994"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFQ61_11825"
     CDS             complement(<1..635)
                     /locus_tag="VFQ61_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HET9955188.1"
                     /translation="MSASTHPSVMPSSQAPSSAGSAAGHAQAEAHGEEENLLEHEYDG
                     IREYDNPLPRWWVLLFWGSFWFALAYILTFHVFDRAPSVKQSYEAEMRVVAEERAQAA
                     LREKVSEESLAKLTGPAVEAGKGIFQARCVQCHAAEGQGLIGPNLTDNSWIHGQGTLM
                     DIYKTVSEGVLAKGMPAWGLQLRPDELRQVVAFVGSIRGKNVPGKAPEGTPV"
     gene            complement(632..832)
                     /locus_tag="VFQ61_11830"
     CDS             complement(632..832)
                     /locus_tag="VFQ61_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955189.1"
                     /translation="MRLSDVMSAMHLTSYAEVALVIFLTVFTIIAIDLFRNKRIESAR
                     FMPFEDGTPETHPSSPTAEKQP"
     gene            complement(871..3153)
                     /gene="ccoN"
                     /locus_tag="VFQ61_11835"
     CDS             complement(871..3153)
                     /gene="ccoN"
                     /locus_tag="VFQ61_11835"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF011053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HET9955190.1"
                     /translation="MSSRTEKFAYDDAVVRKFAFASALWGAVAMLAGLWLALMLVVPT
                     LNFGLPQLTFGRLRPLHTNAAIFAFAGNAIFAAVYYSSQRLLKARMFSDKLSTFHFWG
                     WQAIIVAAALTLPFGITQSKEYAELEWPIDIAIAVVWIAFGVNFFGTIAKRRERHIFV
                     AIWFYIATIITVALLHVFNNLVIPAGFLKSYSVYAGVQDAFMQWWYGHNAVAFFLTTP
                     FLGLMYYFLPKAANRPVFSYRLSILHFWSLVFIYIWAGPHHLHYTALPEWASTLGMLF
                     SLMLWMPSWGGMINGLLTLRGAWNKVSEDPVLKFFVVGITFYGMSTFEGPMLSIKSVN
                     ALSHYTDWTIAHVHGGALGWNGFMSWGMLYWLAPRIFQTKLWSKKLAETHFWIGTIGI
                     LLYIVSIYAAGVTQGLMWRAFDETGRLAYPDFIETVVRLMPMYWVRALGGLLFLGGVI
                     LGIVNLFMTHATRPAKYAPVEAEAPALEPYEGHTPKLVIPRHGTMLDFAYKVRYFAEA
                     QWHRVWERRPLVLTIWVVVSVAVASLFEIIPTFLIRSNVPTIASVHPYTPLELLGRDV
                     YVSEGCYNCHSQMVRPIRAETERYGEYSKPGEFVYDHPFQWGSRRIGPDLHRVGGKYP
                     HLWHVRHMQEPASTTPQSIMPPYPWLFEDQTPFAETQRRVDAMAMLGVPYGDAVNHAE
                     AMAREQAAQVAKEIQAQGGPKGLEDKKIVALIAYLQRLGTDIKKTAPSSPAPGATPRA
                     NTDSPSNTHAALSIEQRSAQ"
     gene            complement(3377..>3900)
                     /locus_tag="VFQ61_11840"
     CDS             complement(3377..>3900)
                     /locus_tag="VFQ61_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HET9955191.1"
                     /translation="MSSGTVNSGTVIWITGLPSSGKSTLAARLVEELRRLGRAACLLD
                     GDEVRDRLVPKLGYSDADRHAFYQTLANLAGLLACQKLDTVVAATAQRSEFRAYARNR
                     APRFLEVYVDTPREECAKRDAKGLYAASAAHSVTNLPGFDSEYEAPLAPDVVAHGGYD
                     DEALARICARVVG"
BASE COUNT          751 a         1180 c         1273 g          696 t
ORIGIN      
        1 acgggggtgc cttcgggcgc cttgccgggc acgttcttgc cgcgaatgct gcccacgaac
       61 gcgactactt ggcgtagttc gtcggggcgc agctgtaggc cccaggcggg catgcctttg
      121 gccaacacac cttcggacac ggtcttgtag atgtccatca aggtgccctg gccgtgaatc
      181 cacgagttgt cggtcaggtt cggtccgatc aagccctggc cttccgccgc gtggcactgc
      241 acgcagcgtg cttggaagat ccctttaccg gcctccacgg cgggtccggt gagcttggcc
      301 aagctctctt cactgacctt ctcacgcagg gcggcctgcg cgcgttcctc ggcgaccacc
      361 cgcatttcgg cttcgtagct ctgcttcacg gacggagcgc gatcgaatac gtgaaaggtc
      421 aaaatgtagg ccagcgcgaa ccagaaggaa ccccagaaca acaagaccca ccaacgcggc
      481 agcgggttgt cgtactcgcg tatgccgtcg tactcgtgtt cgaggaggtt ctcctcttcg
      541 ccgtgggctt ccgcctgggc gtggccggcg gcagagcctg cgctcgacgg cgcctgcgaa
      601 ctcggcatga cggacggatg agtcgaggcg ctcatggctg tttctcggcg gttggcgagg
      661 aggggtgggt ctcgggcgtt ccgtcctcga acggcatgaa tcgggcggat tcgatgcgtt
      721 tgttacggaa caagtcgatg gcgataatcg tgaacacggt caggaagatg accaaggcga
      781 cctccgcgta ggaggtcaag tgcatggcgg acatgacgtc ggacagtcgc atgattgctt
      841 gactccagtc ttgccggtgt aagcgcgtgc ttattgagcg ctgcgctgct cgatggacag
      901 cgcggcgtgc gtgttcgagg gcgagtcggt gttggcgcgc ggcgtggcgc cgggagcggg
      961 gctgctcggg gcggtcttct tgatgtccgt tccgagccgc tgcaggtacg cgatcagggc
     1021 cacgatcttc ttgtcttcga gccccttcgg cccgccttga gcttgaattt ccttcgcaac
     1081 ctgggccgct tgttcgcgag ccatggcctc ggcgtggttc accgcatcac cgtacggaac
     1141 gcccagcatg gccatcgcgt ccacgcgacg ctgggtctcc gcgaacggtg tctggtcctc
     1201 gaacagccac gggtaaggcg gcatgatcga ttgcggggtg gtagaggcgg gttcttgcat
     1261 gtggcgcacg tgccagaggt gcgggtactt gccgccaacg cggtgcagat cgggtccgat
     1321 gcggcgtgag ccccactgga acgggtgatc gtagacgaac tcgcccggct tcgagtactc
     1381 gccgtaacgc tcggtttccg cgcggatcgg ccgcaccatt tgcgagtgac agttgtaaca
     1441 accctccgag acgtacacgt cgcgcccgag cagctcgagc ggcgtgtaag gatgaacgga
     1501 ggcgatcgtc ggcacgttcg agcggatcag gaaggtcggg atgatctcga acagcgaggc
     1561 gaccgcgacc gacaccacca cccagatggt gagtacgagc ggacggcgtt cccaaacgcg
     1621 gtgccactga gcttccgcga agtagcgaac cttgtaagcg aagtcgagca tcgtgccgtg
     1681 gcgcggaatc accagcttcg gggtgtgccc ttcatagggc tcgagcgcgg gcgcttcggc
     1741 ttcgaccggc gcgtacttcg cgggacgcgt cgcgtgggtc atgaacaggt tcacgatgcc
     1801 gaggatcacg ccgcccagga acagcaagcc gccgagcgcg cgtacccaat acatcggcat
     1861 caggcgcacc acggtctcga tgaagtccgg gtaagccagg cgccccgtct cgtcgaacgc
     1921 acgccacatc agcccttgag tgacaccggc cgcgtagatc gacacgatgt agagcaggat
     1981 gccgattgtg ccgatccaga agtgggtttc ggcgagcttc ttggaccaca gcttggtctg
     2041 gaagatgcga ggcgcgagcc agtaaagcat gccccagctc atgaagccgt tccaaccgag
     2101 cgcgccgccg tggacgtggg cgatggtcca gtccgtgtag tgcgagagcg cgttcacgct
     2161 cttgatcgag agcatcgggc cctcgaaggt ggacatgccg tagaacgtga tgccgaccac
     2221 gaagaacttg agcaccggat cttcggacac cttgttccag gcgccacgca acgtgagtag
     2281 accgttgatc atgccgcccc aggaaggcat ccacagcatc aagctgaaca gcatgccgag
     2341 cgtcgaagcc cactccggca gcgccgtgta gtggaggtga tgcgggccgg cccagatgta
     2401 gatgaagacc aacgaccaga agtgcaggat cgagagccgg taggagaaga ccggtcgatt
     2461 ggcggccttg ggcaagaagt aatacatcaa gcccaggaac ggagtggtca ggaagaacgc
     2521 gacggcgttg tgcccgtacc accattgcat gaaggcatcc tgcacgcccg cgtagaccga
     2581 gtagctcttc aggaaccccg ccggaatcac caggttgttg aacacgtgca acaacgcgac
     2641 ggtgatgatg gtggcgatgt agaaccagat cgcgacgaag atgtggcgct cccggcgctt
     2701 ggcgatggtc ccgaagaagt tgaccccgaa cgcgatccaa accaccgcga tcgcgatgtc
     2761 gatgggccac tcgagctccg cgtactcctt ggattgcgta atgccgaagg gcagggtgag
     2821 tgcggccgcc acgatgatgg cttgccaacc ccagaagtgg aatgtactca gcttgtcgct
     2881 gaacatccgc gccttcagta ggcgctggct cgagtagtaa acagccgcaa agatcgcgtt
     2941 gcccgcgaac gcgaagatgg ccgcgttcgt gtggagtggg cgtaagcgac cgaaggtgag
     3001 ttgcggtaag ccgaagttca acgtcggaac tacgagcatc agcgccaacc acaaaccggc
     3061 caacatcgcg acggctcccc agagcgccga ggcgaatgcg aatttcctga caaccgcgtc
     3121 gtcgtacgcg aatttttccg ttcgggagga catccgcggc gctgttgttc aagagccgtg
     3181 ccatcgcggc gcggcccatt cgaagccgat cgggcccagc ctttcacccc tcgggtagta
     3241 tctttacccc gcgatttctg cgcgacgtag aaccagagcg caagcgttgc gcagagacat
     3301 gagcgagctc agcagggggc gcggcagttg tcggttcgag gctcggtcct gacggacgac
     3361 gggcctcacg cgcagatcag ccgacgactc gcgcgcagat acgagcaagt gcctcgtcgt
     3421 cgtacccgcc gtgggcgacc acatccggtg ccagcggggc ctcgtactca ctgtcgaatc
     3481 cgggcaaatt cgtgacgctg tgggcagcgc tcgctgcgta gagccccttg gcgtcgcgtt
     3541 tagcgcattc ctctcgcggg gtgtccacgt aaacctccaa gaaacgtggc gcacgattcc
     3601 tggcatacgc acggaactcc gatcgttgcg ctgtcgcagc tacgacagtg tcgagttttt
     3661 gacaagccag gagcccggcc aagttggcca gggtctgata gaaggcatgc cggtcggcgt
     3721 ccgagtagcc gagcttgggt actaggcggt cgcgaacctc gtccccatcc agcagacagg
     3781 cggcgcggcc aagtcgtcga agctcctcga ccagccgcgc cgccaaagtc gacttacccg
     3841 atgacgggag ccccgtaatc cagatcaccg tacccgagtt caccgtaccc gagctcatgg
//
LOCUS       DASTKU010000336         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_511332, whole genome shotgun sequence.
ACCESSION   DASTKU010000336 DASTKU010000000
VERSION     DASTKU010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511332"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..748)
                     /locus_tag="VFQ61_11845"
     CDS             complement(179..748)
                     /locus_tag="VFQ61_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955192.1"
                     /translation="MRAVCVACLAWALSSGCGGRTENDPIYWSNSGTFGIAGRNAAGQ
                     GGSAGHGGATSNGGAPGTGGAMGRGGAASTGGVAAQGGSRPGTGGVGSGGLGGGQGTD
                     ACLSMCAGMTRACSQVEDWDELSCRRECLNQYAAAGPTCQPKRLEAMACLNRVLARPN
                     AVCEDLAVAIAFGCSSAIEAADECNSRRE"
     gene            complement(802..1728)
                     /locus_tag="VFQ61_11850"
     CDS             complement(802..1728)
                     /locus_tag="VFQ61_11850"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955193.1"
                     /translation="MNLAALDLGSNSFHLLVARVGAGGALTKLASHKEVLRLGAVVQE
                     HGHLPEAHYAAALAAVGTMVSVARAFEAERIIAVGTSALRDASNGRAFCSEAARSFRI
                     PVELISGRREAELAYLGARSGLAGLPERVAVVDIGGGSVEIAVGDGRRCLHIESLPLG
                     FLRLAKALAASGPDGAERLRAEVMREIGPVAARLQALRPEAWIFSGGTARAVGKLVSG
                     DGEPVVAADVERVTQLLERADRSHLVELGVEATRVDAIGLGASVLGALVEGFALHAIR
                     ISPRGLREGVLLRELFPEPWSLQEKRVLPMTA"
     gene            1905..>3482
                     /locus_tag="VFQ61_11855"
     CDS             1905..>3482
                     /locus_tag="VFQ61_11855"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET9955194.1"
                     /translation="MATELFPAGAADVLYLVDLSSYVLRAYHAVAPLSSPTGEPTHAV
                     HGTVSMLERLLRERRPALFAIAMDSGRATFRKEIYAEYKAHRPPAPDDLKIQMNRLEQ
                     IVRAFQVPVLKTDRVEADDLIASCVRTARVEGLRVVIVAGDKDLMQLVGPDVIMYDTM
                     RERVFGVAEVEERFGVRISQVRDYLALTGDSSDNIPGVPSVGPKTAKELLVAYGDLDG
                     IYRQVEQVQRKALRETLQANREQAFLSQRLVTLKDDCDIEFTRERFSRGPRDVGTLRT
                     IYSELGFQRQLTQLELESSSAPAAPADTDTGNEAADAGPAAASANAPAVAPPLAEPER
                     YELVREPQQLLAFLNGARERGHVGVQLFTEGALASRGRLVGAAFCVEPGHAVYAPFAH
                     RNLESGAQISLAEAKALLTPILGDPSIKKFGLDLKSSAVAAARANMNLEGFAFDAGIA
                     SYLLDPEAQHTREALAQRELGVVPTTYDELTRRARGKRVAFDELTPEEARVYAAACAD
                     FAVRLEARMAVRLSEEGL"
BASE COUNT          641 a         1184 c         1074 g          583 t
ORIGIN      
        1 gcgcgaagtt catcagcgat ggtgcagacc aacacgccgg aatcagctgg gaacaaagcc
       61 tgccccacgc ctcccgggcc ttcctagagc tgtacgaacg gcttccgcga tagcgtctcc
      121 ccgtctgcgt ggacccctcg cgtgagctca ctcgcgacgg ggtcaccgag gtcggggctt
      181 actctcgccg tgagttacac tcgtcagcgg cttcgattgc actcgagcac ccgaacgcga
      241 tggccaccgc taggtcttca catacggcgt tcggtctcgc caaaaccctg ttcaaacaag
      301 ccatcgcctc gagtcgcttg ggttggcatg ttggccctgc cgccgcatat tgattcaagc
      361 actcgcgacg gcacgaaagc tcgtcccaat cttcgacctg cgagcaggcg cgcgtcatcc
      421 ccgcgcacat gctgaggcag gcatcggtac cctgtccgcc tccaagcccg ccggaaccaa
      481 cccctccggt tcccggcctg ctgccgccct gagccgctac tcctcccgta ctcgcggcgc
      541 ctcccctccc catggcgccg cccgtacccg gggccccgcc gttgctcgtg gccccgccgt
      601 gaccggcgct accgccttgg cccgcagcgt tgcgcccggc aattccgaag gtacccgagt
      661 tggaccagta aattggatcg ttttcggtcc gaccaccgca acccgaggac agagcccacg
      721 ccagacaagc cacacacacc gcgcgcatca gcgcgatcgt agcgcagcat tagccaaaag
      781 aaagccgcgc cgacacgcgg ctcatgcagt cattggaagc acccgctttt cctgcaatga
      841 ccatggctcc gggaatagct cgcggagcaa tacgccttcg cgcaatccgc gcggggagat
      901 ccggatcgcg tggagagcaa acccttcgac caaggcaccc agcaccgaag ccccgagccc
      961 gattgcatcg acgcgcgttg cctctacgcc aagttccact aggtgcgagc ggtccgctcg
     1021 ttccaggagc tgcgtgaccc gctctacgtc cgccgccacg actggctccc cgtccccaga
     1081 taccagcttt cctactgctc gtgcggtacc gccagagaag atccatgcct ccggacgtaa
     1141 ggcttgcagc cgcgcggcga cggggccgat ttcgcgcatc acttcggcgc gcaaccgctc
     1201 ggcaccatcg gggccgctcg cagcgagagc cttggcgagc cgcagaaaac cgagcggcaa
     1261 gctttcgatg tgcagacaac gacgaccgtc gcccacggca atttccaccg atccgccacc
     1321 gatatccacg acggccacac gctccggtaa cccagcgaga ccgctgcgcg cgcctagata
     1381 cgcgagctcg gcctcacgcc gaccggagat gagttcaacc gggatgcgga aggagcgagc
     1441 ggcctcactg cagaaagccc ggccgttgct tgcgtcacgc agcgcgctcg tgccgacggc
     1501 gatgattcgc tccgcctcga acgcacgtgc cacggacacc atggtgccca ccgcggcaag
     1561 cgctgcggcg tagtgggctt cgggcaaatg cccgtgttct tgtacgaccg ctccgagtcg
     1621 taacacctct ttgtgagagg ccagcttggt gagcgcgccg cccgcgccca cgcgcgccac
     1681 caacaggtgg aatgaattgg agccaagatc caaggctgct aggttcatct gcccgagcag
     1741 agtgaacaat caaggtggcg atggggcgcc cgagtcgtgt cgatctcgtg acgggtgaat
     1801 ttttctaggc ccccctgcgg agcaggaatt cacgctgccg cgggaatcga agggaattgc
     1861 ggcttggtga cgggctccgc ccggagtaag agcgcgtcga gtcgatggct accgaactgt
     1921 ttcccgctgg cgcggcagac gtgctctatc tcgtggacct ctcgagctac gtgctcaggg
     1981 catatcacgc tgtggctccg ctctcgagcc ccaccggcga gccgacccac gccgttcacg
     2041 gcacagtaag tatgctcgaa cggctgctgc gcgagcgtcg gccggcgttg ttcgcgatcg
     2101 cgatggatag cggacgcgcg acttttcgga aggaaattta cgcggagtac aaggcgcatc
     2161 ggcctccggc gcccgatgac ctgaagatcc agatgaatcg gctcgagcag atcgtgcgag
     2221 cttttcaggt tccggttctg aagaccgacc gagtcgaggc agacgatctc atcgcgagct
     2281 gcgtacgcac ggcacgcgtc gaagggctgc gggtcgtcat cgtcgctgga gacaaggatt
     2341 tgatgcagtt ggtcggcccc gacgtgatca tgtatgacac gatgcgtgag cgcgtcttcg
     2401 gggtggccga ggtggaggag cgcttcgggg tgcgaatcag ccaagtccgg gattatctcg
     2461 cgctcactgg cgatagctcc gacaacatcc ccggcgtgcc gtcggtgggg ccaaagacgg
     2521 ccaaagagct gctggtggct tatggcgatc tcgatgggat ctaccgccaa gtcgagcaag
     2581 ttcagcgcaa ggcgttgcga gaaactctgc aggcgaaccg cgagcaagca ttcctgagcc
     2641 agcgcctcgt aacgctgaag gacgattgtg acatcgagtt cacgcgcgag cgcttctccc
     2701 gcggtccgcg cgacgtcggc acgcttcgca ccatttactc cgagcttggc tttcaacgcc
     2761 agctgaccca gttggaactg gagagtagca gcgcccccgc cgcccccgcc gacactgata
     2821 ccggaaacga ggccgccgac gcaggccctg cggctgcgag cgccaacgcg cccgccgtcg
     2881 cgccgccgct cgctgagccc gaacgctacg agctcgttcg cgagccgcaa cagctgctcg
     2941 catttctgaa tggcgcgcgt gagcgcggcc acgtcggcgt gcagttattc accgaaggag
     3001 cgctcgcttc ccgggggcgc ttggtagggg cggcattctg cgtcgagccc ggtcacgcgg
     3061 tctacgcgcc ctttgcacac cgcaatttgg aatctggagc ccagatttct ctcgccgaag
     3121 ccaaagcgct actcacaccg atcctgggcg accccagcat caagaagttc gggctcgatc
     3181 taaagagcag cgccgtggcg gccgcgcgcg ccaacatgaa cctcgaaggg ttcgccttcg
     3241 acgccggcat tgcgagctac ctcttggatc cggaagctca gcatacgcgc gaggcgctag
     3301 cccagcgtga gctaggggtc gtgccgacta cgtacgatga gctcacccga cgagctcggg
     3361 gaaaacgcgt cgcgttcgac gagctgacac cggaggaagc gcgcgtctac gccgccgctt
     3421 gtgccgactt cgcggtgcga ctcgaggcgc gcatggccgt gcgcttgtcc gaggaggggc
     3481 tg
//
LOCUS       DASTKU010000337        13775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_956831, whole genome shotgun sequence.
ACCESSION   DASTKU010000337 DASTKU010000000
VERSION     DASTKU010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13775
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956831"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(513..1370)
                     /locus_tag="VFQ61_11860"
     CDS             complement(513..1370)
                     /locus_tag="VFQ61_11860"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9955195.1"
                     /translation="MSRLCAPARPLPVPVRRFALSVLASEILRDVAVTLQARGIPVMP
                     LKGVLFQRLLYADPTERLLSDVDVLVPESEFEVAIQALRDASFCAPRVGRSLVEVALR
                     SPKGLDVDLHRRLFSPARYRLETDAVFRRATRDDRLLGVPLYIAHPLDTAAHLIGKFV
                     SDHVVADSLKRLRELLLWVEHCDIQPEALASHLDAHGLSRAARYALGCGNEVLGNPFF
                     SATLAALPPDPIGRACAHVARSLMPRWRRTPLAAAPSHLLNDSIFRASASAFWSAFNR
                     WQSTRYTVS"
     gene            2715..3020
                     /locus_tag="VFQ61_11865"
     CDS             2715..3020
                     /locus_tag="VFQ61_11865"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HET9955196.1"
                     /translation="MANELLGALSGAESIEQCDRTASRVIDGKAVIITIDRNELHVLN
                     DVGTFVWQLADGRSLSAIADEVVQEFDVDREQATLDVCAFAQQLCSVGAVTLSKRKD"
     gene            3022..4314
                     /locus_tag="VFQ61_11870"
     CDS             3022..4314
                     /locus_tag="VFQ61_11870"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9955197.1"
                     /translation="MRNVESIRRNKPYQKKNSAAKTVSQLAALSGTPYSAMVEIADRC
                     NEACVHCYQVQGQKGELDTEQWKRIFRELAEMGVLFLTISGGEPTLRKDFIELVAFAR
                     QLRFAVKIYSNALNITPALAAELGRLAVHEVQISLYSHLARVHDSVTRVPGSFDRVVA
                     ATTHLRRVGVKVLLKTPLLSFNSSDFREYVDFVQSLDAEYALDPTLNPREDGSLEPTK
                     FGIRKRDYFAVRRDGRMARLAGTAPKPIRSDAERMPCGACRGSVHVEPNGELRPCTLW
                     NVPTGSALNGVKEAWHNDATATAIRSLTWKDLPDCSVCDLKRYCQRCFADAEQYAGNA
                     LAPYAQACRVARWQYELVHGVEPEIDRDADSCAVMPVGPFRATGSHRFSVAGDDPPSA
                     RRGIQGHEVLSAGTSRAAPQCAISPQLLQIRRHRSRSP"
     gene            4424..8296
                     /locus_tag="VFQ61_11875"
     CDS             4424..8296
                     /locus_tag="VFQ61_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HET9955198.1"
                     /translation="MGTRKRLSAWFAAGTVAIGGGVVGCGGNDQTTVCVGPGCPQYDG
                     AGGDNFFRSGGSGESGGSGERAGSDSAGTANVDPADAGNEPPDADAPGGDAGVPDGGA
                     NPCQIAFLTPEAPASGNLTLDSASDTDGEQCGLEFTIEVSVSSNADTVTLFVNDTPLS
                     TANVVGGTASFNPGLGNRGTVSNALRAKATMADGSTCSVDFPTEILVDCPGPSCNIVF
                     PVASQNGYLNRNQDSNLSAAGLQTGIQVNTEVANVDQTVRLEIDGGFGAAPDQTVVDD
                     NGNGKAIFPSVTLNEGARSVRAECRDAFGLVTLSPVVDWNIDLTPCTLSIDSVAGGAD
                     PITGLNDSDSDAANGLQVALSGQMLGADCRTLRVGSCGDSWTEIALDPPPNGSFSLPI
                     TLAGVTGDVELCAEVSDAAGNLASAQTLVAVRVDSPVVAITSPANGTQFNVSTTCNTS
                     VDVTCSDVGVPVVLLVDGVETATQLCQNDNTASFPLSLATKNNGNPTTVGVQQTADGL
                     VSALASISVQADCQAPVPSISAPGCGTQLALPENDLVAGTPNNLDADVEVSNDGVPLV
                     TLTVSRGASSSDFQADGGAGATTTFSSLDLGGPGNIDLVACATDQAGNQGCTANCALS
                     IAAHPSIAITSPLNLASLSSAGDCAGGTAGLQITVQGTTNAAAGSPVQVSIGSGGLQT
                     VTADGVGFTACVAAPEGNGQTLTVSVTDQATGLSSSASIQVNVDTHAPNTSIAAPTFS
                     VVGRRQGTVRLNWQSVLDSDGDPLAHYRLRCAATDITGASSWNSATEIPVATIPAPTA
                     GNAETEVFGSFQTGKTRFCMIRGEDDAGLITPIAAGLSVVVTNPFNVQEYSVTDDVGV
                     NTNLALTPIGDINGDGRADFAYGATNIGTEIFLGRTDLDDSPSDVLTPDFHITNGGAV
                     GATLGFGAEVSGLGDINGDSRDDFAISARGGNTVFVFFGRASDNPWTNMNLTSMTPCQ
                     ADLCLVGSGLSNGQGLFGWDIDSANFDGTGPMDLVISARILNSTQANGGRVYVLLGGV
                     QLGTSGTTISVPADSADGFIIDPPASRTNFGISVTTMTGTGELDDLVIGANGGAAGST
                     SAGVFRVAGRTHTGAGTGMLTISTTSADEFSVGDIGEFGSPVRALGDFDGDGRADVAI
                     FRNRGSGNGVDLYRGLASGGFDADPSNRLIFPNDLLPGLDDNYGTFMARSFFPAFGLL
                     GDLDGDGISELLIGGTTPNTGTRAVGSLFYGSQGSPGRSRSQSDMTFTPSNAQVVPNF
                     VGDINDDTFPDFAVLDSGSGLNKVVLLY"
     gene            complement(9564..10520)
                     /locus_tag="VFQ61_11880"
     CDS             complement(9564..10520)
                     /locus_tag="VFQ61_11880"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET9955199.1"
                     /translation="MATIQNLARLLIAGLPRSGTTLLATLLAAQRGIHFLTDYFPAFL
                     EAQRRLHKTWRSSLNASERRIALALVRDQFLRVRHPVLVKLNAFSSIDELHRLVLAEL
                     VSGEDHWVGHKLLLSPPQLRATLEETDLHCLVLLRDPRDAALSFYHRTGGGIEAYANN
                     WRDTARACRELQGHSRLLTLRFEDLVAEPEATFRRLGSWLQLELDPQMAQLRFQRSRA
                     HGTTEWHENSAFQDVTARFDRKPIGRWTLQPESLIVRYAGWVTRRELASWNYAPADRY
                     SAGEQLRFALLQALERGEHWAHESVHTAGRLIKRRLQMPQAI"
     gene            complement(10530..11114)
                     /gene="cysC"
                     /locus_tag="VFQ61_11885"
     CDS             complement(10530..11114)
                     /gene="cysC"
                     /locus_tag="VFQ61_11885"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HET9955200.1"
                     /translation="MGSERGATIWLTGLPCSGKSSIARLAAAELQRRGRRVEILDGDA
                     VREQLSRGLGFSREDRDTNVQRIAFVAQLLTRHGVIVLVAAISPYAQARSEARARIGD
                     FIDVHVDCPLAECERRDVKGLYAKARAGTIAAFTGVSDPYEAPTAPELVLQTLEHSPE
                     QSARQLLNYLERQGYVREAESAAGWVVAGARISG"
     gene            11250..11690
                     /locus_tag="VFQ61_11890"
     CDS             11250..11690
                     /locus_tag="VFQ61_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HET9955201.1"
                     /translation="MSSPPAASLPRSDLPARLIIELLRSGQPARFIARGGSMWPSVPA
                     RSQIEVEPCAPAELRVGQLGAFERQGQVVVHRVLRITAEGIQFRGDNLDHRDGIISPS
                     QVLGRARVVARRRWRVRWPERGELRRALRALLRLAAARLSRAWP"
     gene            complement(11773..12189)
                     /locus_tag="VFQ61_11895"
     CDS             complement(11773..12189)
                     /locus_tag="VFQ61_11895"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HET9955202.1"
                     /translation="MTDDAVPAKLAIVRENLEKLERIPQASWQEFDADFRNLDSALHR
                     LQTTIQALIDIACYLVALRGLGVPRTSLDALEKLEAAALLPQGSAQRFAPIVGFRNRI
                     VHLYDRIDPQIVYAILTQNRGDLVELARLLASTLKS"
     gene            complement(12186..12620)
                     /locus_tag="VFQ61_11900"
     CDS             complement(12186..12620)
                     /locus_tag="VFQ61_11900"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9955203.1"
                     /translation="MSGAVLDPAIAGIIGKFPEIAAAWLFGSEARGDAGPTSDIDIAL
                     LFEKRGTTALQAYELLGRLAAQLERVAPGRGIDLVLIEQQGPVFQHRVLEEGRLVYDG
                     DPRRRVDFESDAMVRFLDFEPSHRLASRYATAGLERWLEAQR"
     gene            complement(12734..13144)
                     /locus_tag="VFQ61_11905"
     CDS             complement(12734..13144)
                     /locus_tag="VFQ61_11905"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HET9955204.1"
                     /translation="MTDLDLLEKKLAFVESCVSELRRLAVADKIDSDVKERRFIEHTL
                     QLAIQACLDAASHVVSDHRLGEPSTTRELFTLLARAGRLSQELAQKLSAAAGFRNILV
                     HGYAEVDSRVTRDVLENHLGDLLAFAAELRGQRG"
     gene            complement(13141..13548)
                     /locus_tag="VFQ61_11910"
     CDS             complement(13141..13548)
                     /locus_tag="VFQ61_11910"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9955205.1"
                     /translation="MALRPLSPREIAAAVAARIPSQVAAVYLFGSQARGTARPDSDVD
                     LGLLYVDPPESTLLAQPFLLEAELSVALGRPVQCVVMNEAPPDLVHRILRDQQLLLDR
                     DPALRVRFEVAARNRYFDLKPFLDRYRRRTRVA"
BASE COUNT         2673 a         4376 c         4261 g         2465 t
ORIGIN      
        1 tcctttcgag ccgctgccta cacccgcgcc gtcccgctga acatccccct ctggatccag
       61 cccagcgcca gcgctcgatg gccctcgctc agcgggtcac gcccacgcgt ttctggaatc
      121 agctacgagg cgcggtaaca gtcacccgca agcccatttc tccatgcatt ccgcagggtc
      181 ccgtgcaagc gccaacctcg acgtcgctcg tggaggggtt acactgaacc tgctctgcca
      241 tcatgccttg atggccgccg cgctcgcgag agccaagagc gtcaccgccg accacgtgca
      301 ggcagccctc gcggaagtca gctgacgcgc gggctcgagc gcttcgccca gctgcaccac
      361 aagtcccgac ggggagtcct tcctcgccgg gacttcccta tttcggccac cacaccctcg
      421 gcccacccgg gcgccgcagt gtgagaaacc atcagctcgc catcacgacc gaattcgaga
      481 aactcccggt ggaatagcgt gagaaacaac agtcaggaaa ccgtgtagcg cgtactttgc
      541 cagcgattga atgcggacca gaaggcggag gcgcttgccc gaaaaatgga atcgtttaga
      601 aggtgggagg gagccgcggc caggggggtc cgacgccagc gcggcattaa acttcgagcg
      661 acgtgggcac aggctcggcc gatcggatcg ggcgggagtg cggccagcgt tgcgctaaaa
      721 aatggattcc cgaggacttc attaccgcag ccaagagcat accgcgccgc ccgactcaac
      781 ccatgagcgt ccaggtgact ggccagagcc tctggttgga tgtcgcagtg ctcaacccaa
      841 agcagaagct cacgtagccg tttcaagctg tccgccacca cgtgatctga cacgaacttg
      901 ccaatcaagt gcgctgccgt atcgagagga tgagcgatat acagcggcac gccgagcagg
      961 cgatcatctc gcgtcgcgcg tctaaaaacc gcgtctgtct ccaaccgata gcgagccggg
     1021 ctgaacagcc gccggtgcag gtcgacatcg agccctttgg gcgagcgtag ggccacttct
     1081 accaagctcc gacctaccct cggtgcgcag aatgaggcgt cacgaagagc ctggatggcg
     1141 acctcgaact cgctctcagg aaccaagacg tcgacatccg aaagcaatcg ttcggtcgga
     1201 tcagcgtaca ggagccgctg gaacaacaca cccttgagcg gcatcacggg aatgccccgg
     1261 gcctgtagcg tcacagcgac gtcgcgcaga atttcggacg ccagcaccga aagcgcgaag
     1321 cgccgaactg gcacggggag aggacgagcg ggagcgcaga gtcgcgacat cgaaacccgg
     1381 gattagcacg tagtttagag ccgtgggctg ccggctcgcc atgcggcgcc gaacttagcc
     1441 tccgacacgc cgaccgcggg tacccaactc gctggtgctc gcgatcgcaa ggacaggccg
     1501 cccaactttg cccacttacc cagggctgga ttacatccaa aaccctacac gtggcggaag
     1561 ggtgggcaag cgtgcgcgtg accgtggtgg caagcgacca ccggacttga cgcgggggtg
     1621 agctaaggcg agcattcgat tcgcttagtg gctaattcga cacgatgagc tttgaaaagc
     1681 aaacaccccg cgcgtgttgg cacactctgg ctgcagtagc ttctagaagc tggttgccga
     1741 ccgtactccg atgaagctct tattcgcagg cggccgcttc gatctccggg ggccttccca
     1801 actcctacag cagtttgagc gcgtaccgct gagccaccgc atttcggaac tgcggagtga
     1861 agttcagcaa caatcgagct ggccagcgac tgaatccctt gacgacaacg gcaccggaaa
     1921 cacggctctc tcgaacgtcg agtgtattct ctcttcactg ggagaccgga actcgacgga
     1981 acgcgcgcat ctcgacgcac gcgctgtcga gtgggaatgg acgggcgcgt ccggctggtt
     2041 gcgcacgcgt ttggcgatgg cacgcgtgcg agccacccac agcggcttgt gcgccgaagc
     2101 caatattgct cccggggttc gaagcgcaga gagcctcctc accggtctcg ccgccttgct
     2161 cctcgatcag gctggaggag tgattcttca cgccgccagt gtcgatctac cagcaggagt
     2221 ggtggcgatg atcggcccgt caggggcggg caagagcacc gcctgtctgc aggtagacgg
     2281 agccaagtca ttttctgttg atcggctggc cgttagaccc attccctgcg gcggctggat
     2341 ggcgtatccc ctaccggggg gaacagattc cagtcagcgt cttcctccct gcgagtttct
     2401 gcagcggcct ttgcgggcta tctttcgagt tcgccaaacc gccggctgcg cccgaatcga
     2461 ggcctgcaag cgtccactcg cactggcgat gctgagggag tcctcgttac acacgtcgcg
     2521 ctcggctgag gctgaaaacg accttttgga gcggctccaa ctgctgacac aacaagtacc
     2581 gttcgcaatt ctccattcca gcctcgggtc tttgcttcgc cccctgctcg accgttggtt
     2641 gtcgacgcgg tcgcaagtat cctcgggctg acgcaatgag atgatctggg cgcatgacac
     2701 ggagaacgca ataaatggct aacgagttgt tgggagcact ctccggagcg gagagcatcg
     2761 agcaatgcga ccgcacggca agccgcgtaa ttgacggaaa ggccgtcatc atcacgattg
     2821 accgcaatga gttgcacgta ctcaatgacg ttggaacgtt cgtctggcag ctggcggatg
     2881 gccgttcgct atcggccatt gctgacgaag ttgtgcaaga gttcgacgtc gatcgggagc
     2941 aagccacgtt ggacgtatgc gcattcgccc aacaactgtg ctcggtgggg gctgtaacgc
     3001 tttccaagag gaaagactga cgtgcgcaac gttgagtcga ttcgacgtaa taagccgtac
     3061 cagaagaaaa acagcgcggc gaagacggtg tctcagttgg ccgcgttgtc aggaacaccc
     3121 tacagcgcga tggtggagat cgcggaccgc tgcaatgaag cctgtgttca ctgctatcag
     3181 gttcaaggtc agaaaggcga actcgacacg gaacagtgga agcgcatctt ccgcgagttg
     3241 gcggaaatgg gagtcctttt cctaaccatt tccggcggcg aacctacgct tcgcaaggac
     3301 tttatcgagc ttgttgcgtt cgctcgacaa ctgcgttttg ccgtcaaaat ttactccaac
     3361 gcgctcaaca tcaccccggc actggcggcg gaactcggtc gtctagcggt acacgaggtg
     3421 cagatcagct tgtactcaca cctcgcgcgc gtgcacgatt ctgtcacacg cgttccaggg
     3481 tcctttgacc gcgtcgtggc tgcaacaact cacctgcgcc gcgtaggcgt gaaggttctg
     3541 ctcaagaccc ccctactgag tttcaactcc tcagattttc gcgaatatgt ggacttcgtc
     3601 cagagcctgg acgccgagta cgccctcgat cccacgctca atccacgtga ggatggtagt
     3661 ctcgaaccca ccaagtttgg catacggaag cgcgactact ttgccgtaag acgcgacggg
     3721 agaatggcca ggctggcggg aaccgcgccc aagccgatac gttccgatgc cgagaggatg
     3781 ccttgcggtg cctgccgagg ctcagtccat gtggaaccga acggcgagct gcggccatgt
     3841 acgctctgga acgttccgac gggcagcgca ctaaatggtg ttaaggaggc ttggcacaat
     3901 gacgccacgg cgacggcgat tcgctcgctc acctggaagg atttgcccga ttgcagtgtg
     3961 tgtgatctga agcgctactg ccaacgctgc ttcgccgacg ccgaacagta cgccggcaac
     4021 gcactcgcac cttatgcgca ggcctgtcgg gtggcacgct ggcaatacga actggtgcac
     4081 ggtgtcgagc cagaaattga tcgcgacgcc gactcgtgtg cggtcatgcc cgtgggacca
     4141 tttcgagcga cgggctcgca tcgtttctcg gtcgccggag atgatccgcc ttctgctcgc
     4201 cggggcattc aggggcacga agtgctctca gcgggcacat ccagagccgc gccccaatgt
     4261 gccatttccc cccagctcct gcaaattcgc cgacacaggt cccgctcccc ttaggcgctc
     4321 tcccagcaag cccgtctagc aactaacaaa accggatcgc tccgggagct accatttctg
     4381 ggctccgatg tgaggggttg gggtcaggcg gagacaacca cgaatgggca cgaggaagcg
     4441 cttatcagct tggtttgcag ctggaaccgt cgctatcggt gggggagtag tcgggtgcgg
     4501 cgggaacgat cagaccaccg tctgtgtcgg ccccggctgc ccacagtatg acggcgctgg
     4561 cggcgacaat ttctttcgca gcggcggttc cggtgagagc ggcggctccg gtgagcgcgc
     4621 tgggagcgac agtgccggca cggcaaacgt cgaccctgcg gatgcaggca acgaaccacc
     4681 ggatgcagac gcgcccggag gggacgcggg tgtccccgac ggcggggcaa atccttgcca
     4741 gatcgccttc ctgactcctg aagcgccggc gtcgggtaat cttaccttgg acagtgccag
     4801 cgataccgat ggcgagcagt gcggcttgga gttcactatc gaggtcagcg tcagcagcaa
     4861 cgcagatacg gtcacgctgt tcgtcaatga tactccgctc agcaccgcga acgtcgtggg
     4921 tgggacagcc agcttcaacc cagggttggg gaaccgcggc accgtctcaa acgcgctgcg
     4981 cgcgaaagca acaatggcgg acggtagcac gtgctcggtg gacttcccaa ctgagatcct
     5041 cgtcgactgc cccggaccga gctgcaatat cgtttttcct gtagccagtc agaatggcta
     5101 tctaaatagg aatcaagata gcaacctttc cgcagcgggc ctgcaaacgg gcatccaagt
     5161 caacacggag gtagcgaacg tcgaccagac cgtgcgcctc gagatcgacg gtggctttgg
     5221 tgcggcacct gatcagacgg tggttgacga caacggcaac ggcaaggcga tcttcccgag
     5281 tgtcacccta aatgaaggcg ctcgcagcgt gcgcgcagaa tgccgcgatg cctttgggct
     5341 tgtcacgctc tcgccggtcg tggattggaa tatcgatctg acgccttgta ccctctccat
     5401 cgacagcgtc gctggggggg ctgaccccat caccgggctc aatgacagcg acagtgacgc
     5461 cgccaacggg ctccaggtag cactgagcgg gcagatgctg ggtgcggact gccgcacgct
     5521 gcgagtgggg tcctgcggag acagctggac cgagatcgct ttggacccgc cgccgaatgg
     5581 gagtttttca ctgccgatta cgctggccgg tgtgaccggc gacgtcgagc tgtgcgcgga
     5641 agtgagcgac gccgcaggga atctggcatc ggcacagacg ctagtggcgg ttcgtgtcga
     5701 ttcgcctgtg gtcgccatca cctcccccgc caatggaaca caattcaacg tctccaccac
     5761 ctgcaacacc agtgtcgacg tgacttgctc ggacgtgggg gttccggtcg tgctgctggt
     5821 agatggcgtg gagaccgcca cgcaactctg ccagaacgat aacaccgcta gcttccctct
     5881 cagtctcgct accaagaaca acggcaaccc tacgactgtt ggcgttcaac aaaccgcgga
     5941 tggcctcgtc agcgcccttg cgagcattag cgtacaggcg gattgccagg ctcctgtccc
     6001 cagtatctca gcgccaggtt gtgggacgca gctggcgctc cccgaaaacg atctggttgc
     6061 cggcactccg aacaacctcg acgcggacgt ggaggtatcc aacgatggtg tgcccctcgt
     6121 cactctgacc gtcagccgtg gtgcatccag ctcggatttt caagccgacg gcggcgccgg
     6181 cgcgacgacc acattctcgt cactcgacct gggtggtccc ggcaacatcg accttgtcgc
     6241 gtgtgctacg gaccaggcgg gcaatcaagg ttgcacggcg aactgcgcgt taagcattgc
     6301 cgctcatccg agcatcgcca tcacaagccc attgaatctc gcttctctgt cgtccgccgg
     6361 cgattgcgcc ggcggcacgg cgggtctgca aattacggtt cagggaacca cgaacgccgc
     6421 tgcaggcagt ccggttcaag tgagcatcgg ctcgggcggc ctccagactg taacggcaga
     6481 tggcgtgggc tttactgcct gcgtggcggc accggagggc aacggtcaga ccctgaccgt
     6541 gtcggtcacc gatcaagcta ccgggctcag cagcagcgcg tcgatacagg taaatgttga
     6601 cacccacgcg cccaatacat cgattgccgc gccgaccttc agcgtagtgg gacgccgtca
     6661 gggaaccgtt cggctgaact ggcagagcgt gctcgattcc gatggtgacc cactcgcgca
     6721 ctatcggctg cgctgtgccg caaccgacat cacgggtgca tccagttgga atagcgctac
     6781 ggagattccg gtcgccacca ttcccgcccc gaccgcgggc aacgcggaaa cggaggtctt
     6841 tggctccttt caaacgggga agactcgctt ttgcatgatc cgtggcgagg acgatgccgg
     6901 cctgatcact ccgattgcgg ccggactaag cgtagtggtg acgaatccct tcaatgttca
     6961 ggaatactcc gtgacggatg acgtaggggt aaatacaaac ctcgcactca ccccgatcgg
     7021 ggatatcaac ggagacgggc gggcggactt cgcctatggt gctaccaaca tcgggaccga
     7081 gatctttctt ggccgcacgg atctggatga cagcccgagc gatgtgttga cccccgattt
     7141 ccacataacg aacggggggg ctgtcggcgc cacgctcggg tttggcgcgg aggtctctgg
     7201 cctcggcgac atcaacggag actcacgcga cgacttcgcc atcagtgcgc gcggcggcaa
     7261 tactgttttc gtattctttg gccgagcgtc tgacaaccct tggactaaca tgaacctgac
     7321 cagcatgact ccctgccagg cggatctctg cttggtgggc tccggactta gcaacgggca
     7381 ggggttgttc ggttgggata tcgactcggc gaacttcgat ggcaccggtc cgatggacct
     7441 agtgatttcg gcgcgtattt tgaacagtac acaagccaac ggtggccgcg tctacgtgct
     7501 cctgggtggc gtacagctag ggacgtcggg aacgaccatc agcgttccgg cggacagcgc
     7561 cgatgggttc atcatcgatc cacctgcctc tcgcaccaac ttcgggatca gcgtcaccac
     7621 gatgacggga acgggtgaac tcgacgacct ggtcattggt gccaacgggg gagcggctgg
     7681 ctccacctcc gctggcgtct tccgcgttgc cggtcggact cataccggcg ctggaacggg
     7741 aatgttgacc atcagcacaa ccagcgcgga cgagttctcg gtgggtgaca tcggtgagtt
     7801 cggcagcccc gttcgagcgc tgggagattt cgatggcgac ggtcgagccg acgttgccat
     7861 cttccgcaac cgcggctcgg gcaatggggt cgacctgtac cgcggacttg caagcggtgg
     7921 cttcgacgcg gacccatcca atagactcat cttccccaat gacttgcttc cggggctgga
     7981 cgataactac ggcaccttca tggctcggag cttcttccct gccttcgggc tcctcgggga
     8041 cttggatgga gatggaattt cggagttgct catcggtggg accacgccaa acaccgggac
     8101 gcgggcggtt ggtagcctat tctatggaag ccaaggtagt cctggtcgct cccgctctca
     8161 gtccgacatg acctttacgc ccagcaacgc ccaggtagta cccaacttcg tgggcgacat
     8221 caacgatgat accttccccg actttgccgt gttagactca gggtcaggcc tcaataaggt
     8281 cgtgctcctc tactaagtgc tgcgaccgaa gaatggtcat gctgcggtcc atgccaccag
     8341 atggcagctc agacacgggg gtgggcagct gagccaagcg gcgactccgg tacgggaact
     8401 gacgctgcgg cggacggaga caagggagcc tcgacgacag aagtaatggc gaaacaccta
     8461 gtgcacagga gcgtgctacg ttctgggggg gcgtccgtgc tactacggca tggccagtcc
     8521 gttttgagac gcgacactat tgcgccgaag ttggcacgga aaatcgcctg agattactga
     8581 gaacggtgat gaaggatcgg aaggctcaac cccaaagagc agatatggca cggcctcgcc
     8641 gcccctatgc tgcacccacc atcgaggaaa cgtcgcaatt cgaaaccctt gcgctcgcct
     8701 gcacaaaatc cgatccggat aactgcggca acgcctatgc tcccgatcca ggcagctata
     8761 gcggaagcta gcggcaagct caacgtgctc tgctctgcac gccatcgagc ttgcggcata
     8821 tggaacgtgc tgagccatcc cgacgagcga tcccgttctg tcggataact gtggtattgg
     8881 cgaggtttac tgacttacct atgccccgcg ctgagcacgc cgcggcatcg ccagccacag
     8941 cgcttgattt tgatcccagt gtcccgccga gctcggcaaa ttttgggctt gcgcagcccg
     9001 ctgtgttttg accgcaatga tgggccaagt cccctgaaac gcgaaggtct cgcgctattc
     9061 ggtcattcca tgtcgactct ctggaatcca agcgggagca acctgagcgt cgagattgca
     9121 ctctgccttc ggctgcgggc tctgcaacgg ctggagctgt tcggctggta taccgacggc
     9181 gctcttcgac gataacagat ccgatggggc ctgctggtct caacacgtgt ctgaacagcg
     9241 cattgggctt gtaactaggc cgaaatgggc agcgccgtca cccccgacta cgttggagcg
     9301 gtgatgaagg atcgccacgc gcaaccgaag caggcagatg tagctcggct ccgccgacgg
     9361 tacgcggccc ccgctatcga cgagacctcg ccattcgaaa ctctggcgct ggcgtgcacg
     9421 aaggcggacc cggacagttg cgggtacgct ggtgcgccgg acccaggtag ctacacaggg
     9481 agctaaaggt aaacgccacc ttaagtgcgt cgcgcaccat cacggcagct tcgcttcgtc
     9541 gcccccctgt cgtgataacg tgctcagatc gcctgcggca tctgtagcct tcgcttgatc
     9601 aaacgtccag ccgtgtgcac cgactcgtga gcccaatgct caccgcgctc gagtgcctgc
     9661 aaaagcgcaa atcgaagttg ctcgccggcg gagtagcggt ccgccggtgc atagttccag
     9721 gaggcgagtt cgcggcgggt gacccacccc gcgtaacgga caatcaacga ctccggctgt
     9781 aacgtccagc gaccgatggg ttttcgatcg aagcgggcgg tcacgtcctg gaacgcagag
     9841 ttctcatgcc attccgtcgt gccgtgcgct cggctgcgct ggaagcggag ttgggccatc
     9901 tgtggatcga gctcgagctg gagccagctt cccaaacgtc ggaacgtcgc ctccggctcg
     9961 gccacaaggt cttcaaaacg caaggtcagc aagcgggaat ggccttggag ttcgcgacag
    10021 gcccgagctg tatcacgcca attgttggcg taggcttcga tgccaccgcc cgtgcggtgg
    10081 taaaaggaca aagcggcgtc gcgggggtcc cgcagcaaga ccagacagtg aaggtccgtc
    10141 tcctccaggg tggcccgcag ctgcggcggc gagagcaaga gcttatggcc cacccaatgg
    10201 tcctccccgg agacgagctc cgccagcacc aagcggtgca actcatcgat actggaaaat
    10261 gcattcagct tcaccagcac cgggtgacgc acgcgcaaga actggtcccg cacgagcgcg
    10321 agcgcgatac gccgttcgga tgcatttagc gaagacctcc aagtcttgtg caggcgccgt
    10381 tgcgcctcca agaaggccgg gaagtagtcg gttaggaaat gaatcccccg ctgcgccgcg
    10441 agcagcgtgg ccagaagtgt agttccgctg cgcggcaggc ctgcgatcag caggcgagcg
    10501 agattctgaa tcgtcgccac cgcgaatccc taaccgctaa tgcgggcgcc ggcaaccacc
    10561 catcccgcgg ccgattcagc ctcgcggacg tatccctgcc gctccaggta attgagcaat
    10621 tgccgggcgc tctgttcggg cgagtgctcc agagtctgca gcaccagctc cggggcggtc
    10681 ggggcctcgt acggatcgct cacgccggta aacgcggcga tggttcccgc acgcgccttg
    10741 gcgtacagcc ccttcacgtc gcggcgttcg cactcggcga gcgggcagtc cacgtgcaca
    10801 tcgatgaagt cgccgatgcg cgcgcgcgcc tcgctgcgcg cctgcgcgta ggggctgatg
    10861 gccgccacca gcacgatcac gccgtggcgc gtgagcagct gcgcgacgaa ggcgatgcgc
    10921 tggacgttgg tatcacgatc ctcccggctg aagcccagac ccctggagag ctgctcgcgc
    10981 accgcatccc catccaggat ctccacgcgc cgcccgcgcc gctggagctc cgcggcagcc
    11041 agccgcgcga tgctcgattt gcccgagcag ggcagcccgg tgagccagat cgtcgcgccg
    11101 cgctcgctgc ccatggcgtc atcatgtgaa gcgtccgatc gcgtcgtcca gcgaaaaaaa
    11161 ccctccatcg ccccatcacg cccgcatgct agggtggtcg agcgcggaca gcgcccgtgg
    11221 cgcatcgccg tcacctacct cgtcgcccca tgtcgtctcc gcctgccgcc tccctgcccc
    11281 gctccgatct tccagcacgc ctgatcatcg agctgctgcg cagcgggcaa cccgcccgct
    11341 tcatcgcccg gggcggcagc atgtggccga gcgtgcccgc gcgctcccag atcgaggtcg
    11401 agccctgcgc acccgcggag ctccgggtcg ggcagctcgg ggccttcgag cgccagggcc
    11461 aggtggtcgt ccaccgcgtg ctgcgcatca cggcggaggg gatccagttt cggggcgaca
    11521 acctcgacca ccgcgacggc atcatctccc ccagccaggt gctcgggcgc gcgcgcgtgg
    11581 tggcccgccg gcgctggcgc gtgcgctggc ccgagcgggg cgagctcagg cgcgcgctgc
    11641 gggcgctcct gcgcctggcc gctgcacggc tctcgcgcgc ctggccgtga cgcgcgcccg
    11701 ctcagggtcc cgaggtgccg cgcggtgtcc gctcggagga ggcccggcca aaaaacaaga
    11761 cgcaggtact cttcaggact tcagcgtgga tgcgagcagc cgcgcgagct cgacgaggtc
    11821 tccgcgattt tgcgtcagga tggcgtaaac gatctgggga tcgatgcgat catagaggtg
    11881 cacgatgcga ttgcgaaaac caacaatggg agcgaaacgc tgcgcagatc cctgaggcag
    11941 caacgctgca gcttccagct tttcgagcgc gtcgagactc gttctcggta ctcccaatcc
    12001 ccgcagcgcc accagatagc aagcgatatc gatcaaggcc tgaatcgtcg tctggaggcg
    12061 atgtagcgca ctgtccagat tgcgaaagtc ggcatcaaac tcttgccagg acgcctgggg
    12121 aatgcgctcg agcttctcca ggttctcgcg cacgatcgcg agcttggcag ggaccgcgtc
    12181 atcggtcatc gctgcgcttc cagccagcgc tcaaggcccg ccgttgcata ccgagacgcc
    12241 aatcggtgcg atggttcgaa atcgagaaag cgaaccatcg catccgactc gaagtccacc
    12301 cggcgtcgcg ggtccccatc gtacaccagc ctgccctctt ccaggactcg gtgctggaac
    12361 acgggcccct gctgctcgat cagcaccagg tcgatgccac gcccgggagc caccctttcg
    12421 agctgagcag caaggcggcc tagcagttca taggcctgca atgcagtggt tccgcgcttc
    12481 tcgaacaaca gtgcgatgtc gatgtcgctc gtgggccctg cgtcaccgcg cgcctctgat
    12541 ccaaacagcc aggccgcggc gatctcggga aacttcccga tgattcccgc gatggctgga
    12601 tccagaactg cgccggacat ggagtccact ctagcacagc ggtcgctgat tggtgtccgc
    12661 cacaaccact cgactggccc aacgttcaac gacgaagggt cctccgcctg gcggtagcaa
    12721 ccaccgccga gcttcagccc cgttgccctc gcagctccgc cgcgaaggcg agcaggtcac
    12781 ccaggtggtt ctcgagcacg tcgcgggtca cgcggctgtc gacctcggcg tagccatgca
    12841 cgaggatgtt tctgaatccc gccgccgcac tcagcttctg cgcgagctcc tgcgacaggc
    12901 ggcccgcacg ggcgagcagc gtgaacagct cgcgcgtggt gctgggttca cccaggcgat
    12961 gatccgaaac gacgtgcgag gccgcgtcga gacatgcctg aatggccagc tgcagcgtat
    13021 gctcgatgaa ccggcgctcc ttcacatcgc tgtcgatctt gtccgcgaca gccaggcgcc
    13081 ggagctcgga aacgcagctt tcgacgaagg ccagcttctt ctccagcaga tccagatctg
    13141 tcacgcgacc ctcgtgcgcc ttcgataccg atccagaaag ggcttcaggt cgaagtaccg
    13201 gttgcgggct gctacctcga agcgcacccg gagggcagga tcgcgatcga gcagcagctg
    13261 ctgatcgcgc agaatcctgt gcaccaggtc gggaggagcc tcgttcatca cgacacactg
    13321 cacggggcgg cccaacgcca cgctcagctc ggcttccagc aagaatggct gtgccaggag
    13381 cgtgctctcc ggcggatcga cgtagagcag cccgagatcg acgtcgctgt caggccgcgc
    13441 agttcctcgc gcctggctgc caaacaggta cacggcagcc acctggctgg ggattctggc
    13501 ggcgacggcg gccgcgatct ctcgaggcga aagcggacgc agggccatac gccgagctta
    13561 gtgccggaag ctcggatcgt ccagcgggct gaaatccagg cgctccgcta cggcgtgacc
    13621 gccgccgcgg gccccagatc gaagctgagc gcgatctgat ccgcgctgag cgccgcgcca
    13681 tagatgcgga actcgagcag gctgccctcg aacaggggat cggtcgagaa ttgcgattgg
    13741 cccagccagt tgttcacgtc gctgagctcg gagag
//
LOCUS       DASTKU010000338         6721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1180219, whole genome shotgun sequence.
ACCESSION   DASTKU010000338 DASTKU010000000
VERSION     DASTKU010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180219"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VFQ61_11915"
     CDS             complement(<1..798)
                     /locus_tag="VFQ61_11915"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET9955206.1"
                     /translation="MPIVDLPLAELLTYPGRNPRPDDFDSFWQAALAELDRLDPRIEL
                     RPAKLQASFAECFHLFFTGVGGARVHAKYLRPRQRAARGPALLEFHGYTGHSADFASR
                     LGYVAAGFSVLSLDCRGQGGQSEDVGGVKGTTHSGHIVRGLADTPEKLYYRNVFLDTA
                     QLARIAMQLDGVDPARVGARGASQGGALTLACAALEPRIALAAPVYPFLCDYQRVWEM
                     DLAKDAYLELRTFFRHHDPTHDQVDEWFRRLGYIDIQHLAPRVRGEVS"
     gene            complement(1006..2397)
                     /locus_tag="VFQ61_11920"
     CDS             complement(1006..2397)
                     /locus_tag="VFQ61_11920"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9955207.1"
                     /translation="MEEAGTVLIVDDDATVGQHISSELSGNGWQVELTHDPDAALKLC
                     EQRTFDALILDLLRPASDGASMIERLRAVTPRECRPVTILMSEQLDVRSTVRAVQAGA
                     FDVLQKPVSTADLDERLRAGLRTREREVPAQSMRNPLGDAAERILGETALVASVREQI
                     RSVARFPDLSVVIIGETGTGKELVAEAIHTLSQTGDPFVTVNCAAIPEPLFESELFGH
                     DAGAFTGARGAHAGLLEVAGRGTVFFDELGEMPPTLQPKLLRALETRSFRRVGGSKDL
                     PFRARVVSATNRRLNGREGVVRSDLYFRLAGFTIVTPALRERTTDIDVLARHFLSEFS
                     ERYPEAPAALTEAALDALHAYDWPGNVRELKAVVQQSAVLSRSGSVGAEEVTTVLRER
                     RNEGDPSLAPPASSQSRMLAAPAAGEPLRAFERRMIESAWETSGRNLSAAARTLGLPR
                     TTLRDRLRKYGLI"
     gene            complement(3047..4114)
                     /locus_tag="VFQ61_11925"
     CDS             complement(3047..4114)
                     /locus_tag="VFQ61_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955208.1"
                     /translation="MIHRSLDSVSAAASESVPDAAPNSALGSASVGLDAPVCGGAFGG
                     VKGARRRSVRAMFLAASVLAAGALASCGETEDDSPGPSGSGGLGSGGVSGRGGGGATA
                     RGGASQMGGRSSGGSGGSSARGGRSGTEVAGAAGSEAAGAPSAGASGLPEGGQSGSSE
                     GGQGGESSGPSCGNGVRDSGEMCCTRAFVDTQIEAVFGKAVAQAKGTHSCVEGEQSTV
                     LQVCDTARCIDDEPGCDAEIVGASADYDASTRTVTGNIDMTVRGHMTIGSGRLSVACD
                     FTAVIDDLAYSADVVSVVSADAAQISLENLQLDYTVQVQDCDAVPGADAALGEAAKGL
                     IEPRIVQQISETFQRRIDCPF"
     gene            4718..>6721
                     /locus_tag="VFQ61_11930"
     CDS             4718..>6721
                     /locus_tag="VFQ61_11930"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HET9955209.1"
                     /translation="MSSHPQSPDSAKNPRELLEEAALACVLASSGDSAALERARTALA
                     ALCDGLDGTEHHELALLALTEVANERGNPGPAAERLRRAGEHLDVLQTQLEAPQADAP
                     ELLDPRVSTLPRHPSVRLAASERAEASRTNAPVEPAASATPAVAAPAPMAAPTPTAAT
                     PPSPAAPPSPPTPPSPAVRASVPAGPAGDPELLDLFGDFLAESLEGLDKTDEILLGAE
                     QGLESPENVNALFRVFHTIKGVSAFLGATDVTALAHATESLLDQVRQGQKRLQGSVLD
                     RAFEASSAMRRLLDSAREAVEQGRSIPRDPTIVTLISRINSSTGATVKPPAGASHAPL
                     PNLKGQAPSMRPSIVAAPAPRAPEPRPAVLPAEPAPDSPALEHKKSTAAKAAPPSVHA
                     PSLQDPAAPVDRAVGTSAAEPPTAPLSIAPAKAAAEGHGKIRETVKVDLERVDSVVEM
                     VGELIIMESMVVSSPELASLASLKLKNYLGQLTKISRDLQDVVMRMRMVPVRGVFNKM
                     ARMVRDLGRKTGKLVQLVTSGENTEMDRSMVERLEDPLVHMIRNAIDHAIESPAERRE
                     LGKTAIGTLRLSATHEGGSVAIELTDDGRGLQRDAILKKARSKGLIPESNDGLTDREV
                     FGLIFLPGFSTAAQVSELSGRGVGMDVVKRNIESMRGRVLVNST"
BASE COUNT         1240 a         2329 c         2052 g         1100 t
ORIGIN      
        1 ggacacttcg ccccggacgc ggggggccag atgctgaatg tcgatgtagc ccaaacgtct
       61 aaaccattcg tcgacctgat cgtgcgtcgg atcgtgatgc cgaaagaagg ttcggagttc
      121 caagtaggcg tctttcgcca agtccatctc ccaaacgcgc tggtagtcgc agaggaaggg
      181 ataaaccggc gccgccagcg cgatgcgcgg ctcgagcgcg gcgcaggcca aggtcagtgc
      241 gccgccctga gatgcccctc gagccccgac gcgtgctgga tccacaccat cgagttgcat
      301 cgcgatccgc gccagctgtg ccgtgtccaa aaagacattc cggtagtaga gcttctcggg
      361 cgtatcggcc aagccgcgca cgatgtgccc cgagtgagtg gtgcctttta cgccaccaac
      421 gtcttcgctt tgcccccctt ggcctcggca atcgagcgag aggaccgaaa aaccagccgc
      481 gacgtagccc aggcgagagg caaagtccgc gctgtgcccg gtgtagccgt gaaactcgag
      541 caaggccggg ccgcgcgccg cacgctggcg tgggcggagg tacttcgcgt gaacgcgtgc
      601 gccccccaca cctgtaaaaa acaggtgaaa gcactcggcg aaggacgctt gcagtttggc
      661 cggacggagt tcgatgcgcg gatcgaggcg atcgagctcg gccagtgcag cctgccagaa
      721 tgagtcgaag tcatccggac gaggattacg tcccgggtag gtcagaagct cagcgagcgg
      781 caaatcgacg atcggcatag cgctgcatgc ttacctcaaa ggtaggccgc cgcgttcgcc
      841 caaatgcaag gcaagcctcg catttgggcg cgctccgctc tggtggcgaa cctgcgcgcg
      901 atcatcccca ccgcgcggtc ggttcgagtg cgcccgtgtc caacgtgagt tggaggcccc
      961 gtcactcagg agcgaagcgt gtgctgcatc ccctcggata ctccctcaga tgaggccgta
     1021 cttgcgcaaa cggtcgcgca gcgtggttcg tggcaagccg agcgtgcgag cggctgcact
     1081 caagttgcga ccggaggttt cccaggcgct ctcgatcatg cgtcgctcga atgcccgaag
     1141 cggttcgcct gcggccggcg ccgcgagcat tctggactga ctacttgccg ggggtgccag
     1201 actgggatcg ccctcgttgc gacgttccct cagcacggtc gtgacctcct ccgcgcctac
     1261 gctacccgag cggctgagca ccgccgactg ctgcaccacc gccttgagtt cgcggacgtt
     1321 gccgggccag tcgtaggcgt gcagggcgtc gagtgcggcc tcggtcaggg cggccggcgc
     1381 ctccggataa cgctccgaga attcgctcag gaaatgccga gccagaacgt cgatgtcggt
     1441 cgtgcgctcc ctcaaggccg gcgtcacgat cgtgaagccc gccaagcgga agtacagatc
     1501 cgaacgcacc actccttctc gcccgttcag gcgccggttg gtcgcagaca ccacgcgcgc
     1561 ccggaagggc aggtccttac tgccgcccac gcgccggaaa ctgcgagtct ccaacgcacg
     1621 cagcaacttg ggttgtaagg tcggcggcat ctccccgagt tcgtcgaaga agaccgtacc
     1681 gcgacccgcc acctccaaaa gcccggcgtg agccccgcgc gcacccgtga acgcgcccgc
     1741 gtcgtgcccg aacagctcgc tttcgaagag cggctcggga atcgcagcac aattcacggt
     1801 gacgaacgga tctcccgtct gactcaaggt gtgaatggcc tcggccacca actccttccc
     1861 cgtacccgtt tcgccgatga tgacgacgga cagatccgga aagcgcgcga cactgcgtat
     1921 ctgctcgcgc acggacgcga ccagggccgt ctcgcccagg atgcgttcgg cagcgtcgcc
     1981 gagcgggttc ctcatcgact gggctgggac ttcgcgttca cgcgtccgca gaccggccct
     2041 gagccgctcg tccagatccg cagtcgagac cggcttctgc aagacgtcga atgcgcccgc
     2101 ttgcacggct ctcaccgtgc tgcggacatc cagctgctcc gacatcaaaa tcgtgacggg
     2161 gcggcactca cgcggggtca ccgcgcgcag ccgctcaatc atcgatgctc cgtcgcttgc
     2221 aggccggagt agatccaaaa tcagtgcgtc gaacgttcgc tgctcgcaca gcttcaacgc
     2281 cgcgtcggga tcgtgggtca gctcaacttg ccaaccattg cccgataact cggacgagat
     2341 gtgctgtccg accgtggcgt cgtcgtcgac gattagaacg gtgcctgcct cctccatatt
     2401 tttcctctac cgctgcttgg gtccgcggta cgtagagcat gtctctgaga cttcggatct
     2461 gtcaacactc gtcgagcgtc gtcggttcct ggcaagtcga attcttggaa tagggggtgt
     2521 ctcgcgagtg ggaccatgga gtgtgttcgg atcactccca cttacgtgtc tcgatttggc
     2581 gcaagagaga acgcgctaaa tcgcgcaaaa tcagccaaaa acgccggtag tgagctgatt
     2641 cacgctgctt ctcttacact gctacccggt cgcatttgcc gcgagtttac gggtcagaac
     2701 tggcgcgttg tatccgacac tcgactacgt aacatcagag ccactcacgc ggtccaggcg
     2761 ggtgtctaag ggctgtggcc ctgcacgcgg atgcccgcga aatcgcattt caaacgcgag
     2821 tgccagttcg acacgccccc gcaccgcagg gcgagcccca cgaatttacg tcgcgcctgg
     2881 ttgcgagccg cattcgaatc gcgaaagcgg ggccacttcg gtgtcagtcc cggcgccctc
     2941 ccccgagcat gcgttagccc acgctcattc agccgctcgc gcgcccgcac gccgcgcgaa
     3001 tcgctgcacc acgcgcgcac gagaacggcg gaatgggcgg cgagaatcag aatggacaat
     3061 cgatacgacg ttggaacgtc tcactaatct gctgcacgat acgcggctcg atcaggccct
     3121 tcgccgcctc acccagagcc gcgtccgccc ccggcaccgc gtcgcaatcc tgcacttgaa
     3181 cggtgtaatc gagctgcaga ttctcgagtg agatctgcgc ggcgtccgcc gaaaccacac
     3241 tgaccacgtc cgcactgtac gcgagatcgt caattaccgc ggtgaaatcg caggccaccg
     3301 agagccggcc gctgccaatg gtcatgtgac cacgaaccgt catatcgata ttgccggtga
     3361 ccgtacgcgt gctcgcatcg tagtcggcgc tcgcgcccac gatctccgcg tcgcagccgg
     3421 gttcatcatc gatgcagcgt gcggtgtcgc agacctgaag caccgtcgat tgctcgcctt
     3481 ccacgcagga gtgcgtgccc ttggcttggg ctacggcttt gccgaatacc gcttcgattt
     3541 gagtgtcgac gaaggctcgc gtgcagcaca tttcaccgga gtcacgcacg ccgttgccgc
     3601 aactcggccc gctgctttca ccgccttgac caccctcgga agagccgctc tgaccgccct
     3661 cgggcagccc gctcgcgccc gcgctcggag cacccgccgc ctccgaaccc gccgcgcctg
     3721 ctacttcggt gccgctgcga ccgccgcggg cgctgcttcc tccactccct ccactcgatc
     3781 taccgcccat ctgcgaagca ccaccgcggg ccgtcgctcc accacctccg cgcccgctca
     3841 ctccaccaga accgagcccg ccgctgccgc tcggtcccgg ggaatcgtcc tccgtctcac
     3901 cgcagctcgc gagcgcgccc gccgcgagga cagacgctgc caagaacatg gcacgcacgc
     3961 tgcgccgccg tgcgcctttc acacccccga atgcgccacc gcacaccgga gcatcgaggc
     4021 cgacgctcgc ggagcctaga gcagaattcg gagccgcgtc aggaacggat tcggaagcag
     4081 cggcggaaac agagtcgagc gaacggtgaa tcatgaagag gcgccttacc cccgggacag
     4141 ctacacgggt gaggaatagg agcacgcccc acaatgcggg gcaacaccga tgcccgccca
     4201 ctcgtcacgg aacggcagtg acggacaatt gcgccggcat ggcagccgaa tttcgcaatg
     4261 agctcgaggc agacgtgcat agagctgcac ggcgacggag tttcagcccg gatttcgcgc
     4321 ccacgacgga cgaagtcggc aagccgacgc gacatcgact gcagattggc tgttggcgcg
     4381 cgcagccata gcaaggctat cgcgcccacg tggccccacg gcgaagctga gatcgcgagg
     4441 aattgccggc ttcgaactta ggggcgcgaa atcggtttca ccagcctcga cgcgccccaa
     4501 ctcgcataca tcgagtcagc aacggtgcca tgccccgtcg ttcgtctcct ctggcgatgt
     4561 tcgctcggtc atgagacttc gcctgtgttg cgggcgcgca agtgggagag gttcttaacc
     4621 aagttgagct caaagccgct gatgcatgtg ccgtcgctgt tcgaagccgc tccgctccta
     4681 ggccacacca gcgatgagca gcgcactctg ctcgcccatg tcgtcgcatc cgcaatcacc
     4741 cgactccgcc aaaaatccgc gtgagctcct cgaggaggcc gctctggctt gcgtcctggc
     4801 gagctcgggc gatagcgcag ccctcgagcg cgcgcgaacg gcgctcgccg cgctgtgtga
     4861 cggcctcgac ggcacggagc accacgaact cgctctgctc gcactgaccg aggtcgccaa
     4921 cgagcgcggg aatccgggcc cagccgccga acgcctaaga cgcgccggcg agcacttgga
     4981 tgttctgcaa acccaactgg aagcgcccca ggcggacgcg ccagagttgt tggacccacg
     5041 cgtcagtaca ctcccgcgcc accccagcgt acgtctcgcg gcgagtgaac gagccgaagc
     5101 atcgcgcacg aatgctccgg tcgaacccgc cgcgagcgcc accccggccg tggccgcgcc
     5161 cgcgccgatg gcggcgccga ccccgacggc ggcaactcca ccctcacctg cagccccgcc
     5221 ctcacctccc actccgccct cacctgcggt gcgcgcttcg gttccggcag ggcccgccgg
     5281 ggaccccgag ctactcgatt tgttcggcga cttcctggcg gagagtctgg agggtttgga
     5341 caagacggac gagatcttgc tcggcgccga gcaaggcctc gagtctcccg agaatgtgaa
     5401 cgccctgttt cgggtgttcc acaccatcaa gggcgtgtcc gcttttttgg gcgcgaccga
     5461 cgtgaccgcg ctcgcgcacg ccaccgagag cctgctcgac caggttcgcc aaggccaaaa
     5521 acgactgcaa ggttcggttc tggatcgcgc tttcgaggcc tcctcggcca tgcgccgctt
     5581 gctggactcg gctcgcgaag cggtcgaaca aggtcgctcg atcccgcgcg atccgacaat
     5641 cgtcacgctg atctcgcgca tcaatagctc taccggcgcg accgtgaagc caccggcggg
     5701 agcctcgcac gcgcctctgc cgaacctcaa gggccaggcg ccgagcatgc gcccgagcat
     5761 cgtagcggcg cccgcgccgc gcgcgcccga gccgcgtccc gcggttctgc ccgcagaacc
     5821 ggcgccggac tcacctgcac tcgagcacaa gaaaagtacc gccgcgaaag ccgcaccgcc
     5881 ttcggtgcac gcgccgtccc tccaagaccc tgcggccccg gtcgatcgag ccgtgggcac
     5941 gtccgcggca gagccaccca ccgccccctt gagcatcgcg ccggccaagg ctgcggccga
     6001 gggtcacggc aagatccgcg agaccgtcaa ggtcgatctg gaacgagtcg acagcgtcgt
     6061 cgagatggtc ggcgaactca tcatcatgga atcgatggtg gtgagttctc cggagctcgc
     6121 gagcctggcc tcgctcaagc tcaagaacta cctcggccag ctcacgaaga tcagccgcga
     6181 tctgcaagac gtcgtgatgc ggatgcgcat ggtccccgtg cgcggcgtgt tcaacaagat
     6241 ggcgcgcatg gtgcgcgatc ttggccgaaa aaccggcaag ctcgtgcagc tcgtcacctc
     6301 cggcgagaac accgaaatgg accggagcat ggtggagcgg ctcgaagacc cgctggtgca
     6361 catgatccgc aacgccatcg atcatgcgat cgaatccccg gctgagcgcc gagaactcgg
     6421 caaaaccgcg atcggcacct tacgcctgtc cgcaacccac gagggcggca gcgtcgccat
     6481 cgagctcacc gatgacggcc gtggcctaca gcgagacgcc atcctgaaga aggcccgctc
     6541 caaagggcta atcccagagt cgaacgacgg cttgaccgat cgcgaggtgt tcgggctgat
     6601 tttcttgccg ggcttctcca cggccgctca ggtcagcgag ctctcggggc gcggcgtcgg
     6661 catggacgtc gtcaagcgca atatcgagag catgcgcgga cgggtgctcg tcaactctac
     6721 g
//
LOCUS       DASTKU010000339         4180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1404220, whole genome shotgun sequence.
ACCESSION   DASTKU010000339 DASTKU010000000
VERSION     DASTKU010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1404220"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VFQ61_11935"
     CDS             complement(<1..509)
                     /locus_tag="VFQ61_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955210.1"
                     /translation="MFELRLGMLRQVCLGVSALLFGVACSAEADSADGLEQESVGDAQ
                     QAVTAGAWESWLPMPMNANGFEGPPATCLATPGGALVVGRDRITNRFRVSQFQGNPFS
                     AWTDFGAMTFASKPAAAALDGLFFQPEAQWNRQVAVVGRASDNRYYVRIGRLDSTPPL
                     TSNPTVVHD"
     gene            complement(1361..2524)
                     /locus_tag="VFQ61_11940"
     CDS             complement(1361..2524)
                     /locus_tag="VFQ61_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9955211.1"
                     /translation="MTDGKVTLQGEATGIAGLADGNATPKIMSGIPLSVAGGTNLGTH
                     QSVWNVESGMEFSIASADSLGARVSPRFGGVVYGPGGFYVGLRGGPQLPLSVERHEVV
                     ALSLEGIANIYTGGAFAGKPNFGGVLSLSWEYFGSFNIPSGRPLRSEEGTPARAKLRA
                     GNSWRARTSHAAEQALELTDAERRELGATWLEDARMEHASIAAFAQLTLELLELGAPA
                     RLIEATQRAALDETRHARICFEQAAAHLNMQIEPASLTLPTRDDHRTSYEAVAVSSAL
                     EGCIGERLAAALLRHRARHASSPELARALSGIQRDEERHARLAWEVIQFCIDRCGSKV
                     LRSITAAFATHQNYTYSPTPLLCAHGWPNTQFAETAQHRIRTQVERRIAAMQS"
     gene            3035..3919
                     /locus_tag="VFQ61_11945"
     CDS             3035..3919
                     /locus_tag="VFQ61_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955212.1"
                     /translation="MPSLLHEGIIELIRDRPEVAAKLLSELLHVEIPQFAEARLAEAN
                     LTQLLPVEYRADAVVLLVEGKPVFGIVIEAQLQPDERKSFSWPVYAVTARARYECPCV
                     VIVITPESSLADWASKWIDLGGGMQWRPFVVGPEGIPVITDPQQASRVPELAVLSAMA
                     HGRGSPEVAAAIALAAVEGLAGLPEEPRVLYCAFIESALSDAARKAIQMPLAEKFFSD
                     TQRHAFARGQAEGEIKGRAEGRAEAQAEFVVHVLTIRGLSLSAPQRERILTCQDSEQL
                     MRWLDLAMSCGNVDELLG"
     gene            3954..>4180
                     /locus_tag="VFQ61_11950"
     CDS             3954..>4180
                     /locus_tag="VFQ61_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955213.1"
                     /translation="MASWRWASLTRIAYFKHVVVFVEIRPLTVAQGRSPEPQISCISV
                     IESALSDAARKAIQMPLAEKFFSDTQRHAFA"
BASE COUNT          754 a         1252 c         1350 g          824 t
ORIGIN      
        1 caatcgtgca cgaccgtggg gttcgacgtg agcggaggcg tactatcgag ccgaccgatc
       61 cggacataat atctgttgtc cgacgccctg ccgaccacgg cgacctggcg gttccactgc
      121 gcttccggct gaaagaacaa cccgtcgagc gcagcggcag cgggcttcga ggcgaaggtc
      181 atcgctccga agtccgtcca ggcagagaac gggttaccct ggaactgact gactcgaaag
      241 cgattcgtga tgcgatcacg tccgaccacg agcgcgccac ccggcgtggc gaggcaagtc
      301 gcgggtggtc cttcgaaacc gtttgcgttc atcggcatgg gcagccaaga ctcccaagca
      361 cccgcggtca ccgcttgctg cgcgtctcca acactctctt gttcgagacc atccgccgaa
      421 tcggcttctg cgctgcacgc taccccgaac agtagcgcgc tcacccctaa acacacttga
      481 cgtagcattc ccaatcgtag ttcgaacatg tcgccatact cctatgggtt cttcgcgacc
      541 cctgcttttg cttgtctcac cgcggtcgga gcgcagagcg aatgctgccg ggagaacctc
      601 atatgggtgc tcccacaaaa ctcaattaca ccaggaccac acctctccag atcccgtcgc
      661 tctactccga aagcgtacgg caatataggc tcaccttatt gcgctcagca atcacgattt
      721 gcgcactccg gtcatatcgt gaccgagctg aagctcatgt cgctggtgtc gaactcaccg
      781 ccttccgtca tcggcgcgaa attcggttcg aggccgccga cgaccgggaa ctgactctct
      841 tggggagcgc attgacggct ctccagcgca agttctcaat tacgagccaa cggccttcct
      901 gcctcatgga cagttgtgcc attgcgcaag agtggcgtgt tcacacaacc gacaggtctt
      961 tagggatctc ctcgacggag gtctctcgaa tcggcccgtc tcgatcccgg gttgtgattc
     1021 gcagaacgcg gcattgcgcg actcaccgcg agcgccagcg cggtgcgatt ggcgctcgcg
     1081 agaaatgagg actgagcctt cttctcgctc tagcgcgctt cgcgcccgtg gactccttgg
     1141 cgcagacggg aatggatttc gcgcctcctg ctcagttagc ccgcccagca acatcgccga
     1201 atcaagacca gggacttccc aatgatggcc tgcgacgaac aaatcgtaat cttctatgag
     1261 tcgggccaca tcgctaaatc gagattggcc cggatggctg ccgacgaggt tcgcggcgag
     1321 agacgcgcgg cgcgagcaat gtgcgcaaac cacgacagtg ctaactctgc atggcggcga
     1381 tgcgacgctc aacctgggtg cgaatgcggt gttgagctgt ttcggcgaat tgggtattgg
     1441 gccagccgtg ggcgcagagg aggggagttg gggagtaggt gtagttttgg tgcgttgcga
     1501 atgcggcggt gatgctgcgc aggactttgc tgccacagcg gtcgatgcag aattggatga
     1561 cttcccacgc gaggcgcgcg tgacgttctt cgtcgcgttg gatgccgctt agggcgcgcg
     1621 cgagctcggg gctggaggcg tggcgagcgc ggtggcgcaa cagcgcggcc gcgagccgct
     1681 ctccaatgca gccttccaac gccgagctga cagcgacggc ctcgtacgag gtgcggtggt
     1741 cgtctctcgt gggaagcgtc aggctcgcgg gctcgatctg catgttgaga tgcgcggcgg
     1801 cttgttcgaa gcagatccgc gcgtggcgcg tttcgtccaa cgccgcgcgt tgcgtcgctt
     1861 cgatcagccg ggcgggcgcg ccgagctcga ggagttccag cgtgagctgc gcgaacgcgg
     1921 cgatggaggc gtgttccatg cgcgcgtctt cgagccaggt ggcgccgagc tcgcgacgtt
     1981 ccgcgtcagt gagttcgagc gcctgctccg cggcgtgtga ggtgcgcgcg cgccaggaat
     2041 tgccggcgcg cagttttgcg cgagcgggcg tgccttcctc gctgcgcagc ggccgaccgc
     2101 tgggaatatt gaagctgccg aagtactccc agctcagact gagcacgcct ccgaagttgg
     2161 gttttccggc gaacgcgccg cccgtgtaga tattggcaat gccctcgagc gatagcgcga
     2221 ctacctcatg gcgttcgacg gagagtggca gttgcggtcc gccgcgtagg ccgacgtaga
     2281 agcctccggg gccgtatact acgcccccga agcgtgggct cacgcgtgcc cccagggagt
     2341 ccgctgaagc gatcgaaaac tccatgccgc tttcgacgtt ccaaaccgac tggtgtgtac
     2401 ctaggttggt gccaccggcg acgctcaacg ggatgccaga catgatcttc ggagtggcgt
     2461 tgccatcggc gagcccggcg atgcccgtgg cttcaccctg gagcgtaacc ttcccatcgg
     2521 tcatgatcgt cgctttcggg ccgccgcgca ggctggcgcc gcaaccgggc aatgcgaaca
     2581 agactggtgc aactgaaagc caacggaccc caaccattcc gcgaggctag cgcaacgcgg
     2641 gtggatcagc tgcaagaaaa gtgtccgggg tgccggccgc cgctggcatc cacgacgcac
     2701 gataaccgcg agcctacggc cccggtcgga agggcctacg ttctaggatt tgagtaacgc
     2761 ctcgagctcg gcgacgcggg ctaactttcg gtacgtcgct tttggcgctg gagcggccgc
     2821 gtgcttcttt agcccgcatc tgtgctagac gcgcttggag gcatgacccc gcgcgttgcg
     2881 aagcgtcgct gtcagccatg gattgcgaac tcgcctgcag tgcttgggtc agaatcagat
     2941 gctgagtttg cgcgcacggg atatccctgg agcgtagaca atttttgttg ttttcaggct
     3001 tgctcgttct cttctgaaat gacatgtagc tggtgtgcca tcactgcttc atgaggggat
     3061 catcgagctg attcgcgacc gtccggaggt cgcggcaaag ctgttgagcg agcttttgca
     3121 cgtggagatt cctcagtttg ccgaggcgcg cctcgcggag gcgaacctga cgcagctgct
     3181 gccggtggag taccgggctg acgccgtggt tctgctggtc gagggtaagc cggtgttcgg
     3241 tatcgtgata gaggctcagc tgcagcccga tgagcggaag tccttctcgt ggcccgtgta
     3301 tgcggttacg gctcgggccc gatacgagtg cccgtgcgtg gtgattgtga tcactcctga
     3361 gtccagcctg gcggactggg cttccaaatg gattgacctc ggcgggggca tgcaatggcg
     3421 accctttgtg gtcgggccgg agggaattcc ggtcatcacc gatccgcaac aggcgtcccg
     3481 ggttccggaa ctggcggtgc tgtcggcgat ggcacacggc cggggtagcc cggaagtcgc
     3541 ggccgccatc gcgctggccg cggtggaggg cctcgcgggt cttcccgagg agccgcgggt
     3601 gctatactgc gccttcatcg aatcagccct gagcgacgca gcaagaaagg ccattcagat
     3661 gcccttagcc gagaagtttt tcagcgacac ccagcgccac gcattcgcta gaggtcaggc
     3721 cgagggtgag atcaaaggca gagctgaagg cagagctgag gcgcaagcag agttcgttgt
     3781 gcacgtgctg acgatccgtg ggttgagcct gtcggcccct caacgcgaac ggatactcac
     3841 ctgtcaagat agtgagcagc tcatgcgttg gctggacttg gcgatgtcct gcggcaacgt
     3901 ggacgagctt ctgggttgag gcgaggcttc gcctactgcg ctgggtgtcc agggtggcga
     3961 gctggcgttg ggcatctctg acgagaatcg cctatttcaa acacgtcgtc gtatttgtgg
     4021 agattagacc cctgactgtc gcgcagggga gatccccaga gccgcagata agctgtattt
     4081 ccgtcatcga atcagccctg agcgacgcag caagaaaggc cattcagatg cccttagccg
     4141 agaagttttt cagcgacacc cagcgccacg cattcgctag
//
LOCUS       DASTKU010000340         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1850744, whole genome shotgun sequence.
ACCESSION   DASTKU010000340 DASTKU010000000
VERSION     DASTKU010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850744"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFQ61_11955"
     CDS             complement(<1..620)
                     /locus_tag="VFQ61_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955214.1"
                     /translation="MTGGWFGRRSFASAVFAGGVLATWTSFLHAAPGLLLDATPLLTQ
                     SEANSGWRSVLVRLENPSAQTLRGHVELASRPSWARATWAKTGAEFTTRVPFSLAPKA
                     RVSFEAPTRGFGEGAPTVRVTAVSETNEPLADVVMPSVHSADAVVLDLQNPSRVAAAL
                     RRATFVSDRTPPFASRGRVSELGVSVPQVDPTTSDLILPQIPAGYGG"
     gene            complement(692..1726)
                     /locus_tag="VFQ61_11960"
     CDS             complement(692..1726)
                     /locus_tag="VFQ61_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9955215.1"
                     /translation="MESIEARLAWAQSSCERLKASIGQVIVGQSQVVDQVLSCLIAGG
                     HVLLEGNPGLGKTLLVRTLAACLDLRFSRIQFTPDLMPSDITGNNVLVLNQSGAGTDG
                     QRHFELYRGPIFGQVVLADEINRATPKTQSALLEAMQEHAVTIAGTRHVLEEPFFVLA
                     TENPIEMEGTYPLPEAQLDRFLFKVLVPSPDETGLQRILRQTTGAPTAEPTRILNVAE
                     VLELRILCRSVEVSDAVLGYAARLVSATSPDSERATARVKYAVRYGAGVRGAQALILG
                     AKAGALLQGRGHVSFADVQQVALPVLRHRILRSFEGEADGVSSDQVIRELLESVPTLP
                     SHVENAVSAR"
     gene            complement(1900..3057)
                     /locus_tag="VFQ61_11965"
     CDS             complement(1900..3057)
                     /locus_tag="VFQ61_11965"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955216.1"
                     /translation="MAEEKNTDRVRGRLASSPGGGDEATAGRATVEGAATNATALDRM
                     TDGLRSWRRAEAGLLIAPFERHPEAARYLQITRHEALRQGLVRGADVRRFERFVVLDA
                     LIYPRASFEMLVVCGGLNQWLFLLDDACDTHPEIATSEPRLRALIEPAFQALISGRLP
                     GPSSPFAEFSALVGARLRARASARLWNRFLEHTFDCLFRGCLSSSQASSCALPLDTLR
                     ELRRLDSKVDLLIDLVELSRTGELNPEEQSQPAVRELRELVVDQLTLLNDLVSYNREV
                     DGTGSRFNLLHALRTQRWDGSLERAKVALVSELNALAARFEAVASTLGGRLALYAEGM
                     RHLLGGYQRFAFHSGRYHHPAAHLPELRQIPLDDPGFAAARETESMEALPA"
BASE COUNT          619 a         1095 c         1020 g          561 t
ORIGIN      
        1 ccgccatagc cagccggaat ctgcggcaaa attagatcgg aagtcgtcgg gtcgacttgc
       61 ggaacggaga cgccgagctc gctgacgcga ccgcgactcg caaagggcgg cgtccgatcc
      121 gaaacgaaag tcgcacgccg cagggcggcc gcaacccggc ttgggttctg caggtccaag
      181 accacggcgt cggccgagtg cacgctcggc atcaccacat ctgccagcgg ctcgttggtc
      241 tcagagaccg ccgtcacgcg caccgtcggc gcgccctcgc cgaagccgcg cgtgggagcc
      301 tcgaagctga cccgagcctt aggggccaaa gagaagggga cgcgcgtggt gaactcggcg
      361 ccagtttttg cccaggtggc ccgcgcccaa ctcgggcggc tcgccagctc cacatgcccg
      421 cgtagcgtct gggcggaagg gttttcgagc cgcaccagga cgctgcgcca ccccgagttt
      481 gcttcggact gggtcagaag cggcgtcgca tccaatagca agccgggggc cgcgtgtagg
      541 aaggaggtcc aagttgcgag cacgccaccc gcaaatacgg cgctcgcaaa cgagcgtctc
      601 ccaaaccagc caccggtcaa cgcaacactc ctgaacgtag cggagcccgg ccgtcctaaa
      661 atcctcgatc cccgacgccg ctgccggtcc ttcatcgggc gctcaccgcg ttttccacgt
      721 ggctcggaag tgtgggcacc gactcgagca actcgcggat tacttgatcg gaagaaacgc
      781 cgtcggcttc accctcgaag ctgcgcaaga tgcggtgtcg cagaacgggc agcgcgacct
      841 gctgcacgtc cgcgaaagaa acgtggcccc gtccttgaag aagcgcgccg gctttcgcgc
      901 ccaaaatcaa ggcctgggcc ccgcgtacgc ccgcaccata ccgcaccgcg tatttcacgc
      961 gcgcggttgc gcgctccgaa tccggagacg tggcgctcac gaggcgggcc gcataaccga
     1021 gcactgcgtc cgaaacctcc acgctgcggc acagaatcct cagctccaac acttcggcca
     1081 cgttgaggat gcgcgtgggc tctgccgtgg gcgcacccgt ggtttggcgc aaaattcgtt
     1141 gaagcccagt ttcgtcggga gagggaacca acaccttgaa caggaaccgg tcgagctgcg
     1201 cctccggcaa tggataggtg ccctccatct cgatcggatt ttcggtggcc aatacgaaga
     1261 agggctcttc gagcacgtgc cgggtgcccg cgatggtcac ggcatgctcc tgcatggcct
     1321 cgagtagcgc actctgggtc ttgggcgtgg ctcgattgat ttcgtccgcc agcaccactt
     1381 ggccgaagat ggggccacgg tacagctcga aatgacgctg tccgtcggtg cccgcgcccg
     1441 attgattgag cacgagcaca ttgttccccg tgatatcgct cggcatcaaa tccggtgtga
     1501 actggatgcg tgaaaagcgc aggtccaagc aagccgccag cgtgcgcacg agcagtgtct
     1561 tgccgagccc ggggttcccc tcgagcaaga cgtgtccgcc cgcgatcaaa cacgaaagca
     1621 cctgatcgac cacctgtgat tgcccgacga tcacttggcc gatgctggcc ttgaggcgtt
     1681 cacagctgct ttgggcccag gccaagcggg cctcgatact ttccatcgcc cgctactata
     1741 ctcagaattc tgagcggcgg gctcggcgtc acactcctag cgcctcacga gcgtgacgtt
     1801 ctagcggctc gtcattgggg cttggcgcac cgtccgagtg gaggcaccag cccgttcgga
     1861 ttccgtggcc ccgtcggacg tttggtgacg tccacggcat caagccggca gagcttccat
     1921 cgattccgtc tcacgcgccg ccgcaaaccc cgggtcgtct agcgggatct gccgcagctc
     1981 gggcaagtgc gcagcggggt gatggtaccg gccgctatgg aaagcgaagc gttggtatcc
     2041 tccgagcaag tggcgcatgc cctcggcata gagtgccagc cgacccccta gggtagatgc
     2101 gaccgcctcg aagcgagcgg cgagcgcgtt cagctccgaa accagcgcga ccttggcgcg
     2161 ctccagggac ccatcccagc gctgagttcg cagcgcgtgc agtaggttga agcggctccc
     2221 agtaccatcg acctctcgat tgtacgagac gagatcattc aagagagtca gctggtccac
     2281 caccaactct cggagttccc ggaccgctgg ctgcgactgt tcttcggggt tcaactcacc
     2341 cgtgcgggac aactcgacca agtcgatcag caggtccact ttggagtcca ggcgtcggag
     2401 ctcgcgcagg gtgtccaggg gcagcgcgca gctcgaggct tggctcgagc tcagacaacc
     2461 acggaacaag cagtcgaagg tgtgctccag gaaccgattc cagagccgag cggatgctcg
     2521 cgcgcgcagt cgcgccccca ccagcgcgct gaactccgcg aacgggctgg atggtccggg
     2581 taagcgcccc gagatcaggg cctgaaaagc cggctcaatc aaggctctca gtcgcggttc
     2641 ggaggtagcg atttcgggat gcgtgtcgca ggcatcgtcc agcaggaaca accactggtt
     2701 gagcccaccg cagaccacca acatctcgaa gctcgctcgc ggatagatca gcgcatcgag
     2761 caccacgaag cgttcgaagc gccggacgtc cgctccgcga accagacctt gacgtagagc
     2821 ttcgtgacga gtgatctgca ggtaacgagc cgcttccggg tgtctttcga atggggcgat
     2881 caacaagccc gcctctgcgc gccgccagct gcgcaggcca tccgtcatgc ggtcgagcgc
     2941 ggtggcgttc gttgctgctc cctcgaccgt tgctcggcct gccgtcgctt cgtcaccgcc
     3001 gcccgggctg gatgctagcc gccctcgtac tcgatccgtg tttttctcct cagccatctg
     3061 caaccccaac ctgaatcgcc gagccgcgga gttcgagtca cgacgctcaa tcccgaagcc
     3121 ctgcttccca aactcacatc accaagtgga acagctagcg cctcgccaag atcacgtcaa
     3181 gccgcgcagt ggccggctgt tttcgccggc tgctctgcga aggctggttg gggcacgtgt
     3241 ctttctatga tgcgctcgta ctcgggaaac acccggtggc tcgggaaaga ctgcg
//
LOCUS       DASTKU010000341         9107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_957737, whole genome shotgun sequence.
ACCESSION   DASTKU010000341 DASTKU010000000
VERSION     DASTKU010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9107
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957737"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2359)
                     /locus_tag="VFQ61_11970"
     CDS             complement(<1..2359)
                     /locus_tag="VFQ61_11970"
                     /inference="COORDINATES: protein motif:HMM:NF016615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HET9955217.1"
                     /translation="MPLPAVRPATLDSTGSDHELKVAPRLAVAESPCIGTEPSQVPPI
                     RHAPQVFTRAARWPVQTLEALAAPELAELAGQLDESDPAAWSEYVVRFDHERQEQLRT
                     LWRLTAQDPSFMRALVVSNPDLAQRALAAEGILKQNKRARHLQTTLYRYLARAAGRVE
                     PCELWAGVTLANFGESTRVEAAPAPVRFAPDLRVFQSLLRALAAREDYRKVGLWKVNV
                     TLERDVEGYFTFWARNAQGQVSRKRMRPPAAVAQVLKTIRGVPAASFGDLCERAGADS
                     GLDVDTVEAVLLACIDGGILVGGLDLPLRYSSSWEALAQAACALHGPERAAWWVAYRK
                     LRRFCRKLERELDALAPSELLLRFDRAAQVIRELAADLNLGCPELPRVVLRCDAGLPF
                     RVTLGVEAQRAIATAIAEYEQFQRKYGLGYTLRSALVDEYVDGALLRRPLSAVEPLVP
                     EPSPTEPLLTWESHERLMRAPEAYRQRLQSWSELLQGQPVTLDGNRATETAVSPLASL
                     IFGLNGSRVVIQGATDEVAAIYSRYVPLWNPITRAPRDPFYGWFTLQLAEISHRTGLV
                     LCELLNPCEANPNALVRPRYTEQCVSVWGAVAGELGLFRAETFVDSRSRTLLLGTAPQ
                     TQPAAVLSFASANLGYRDPVCQALLFTGFRAGRLAHLRAESMSFSIELESGRPSPEIK
                     TPQGHVIRDRRTVVSGADLTQLIEASPELRFVRWQKLARRHGWAAMLHVARDFETPLL
                     VPRDCPIALQALFEGASHVSALVVEERADQAFLEDDHGRRYITQFV"
     gene            complement(2634..2807)
                     /locus_tag="VFQ61_11975"
     CDS             complement(2634..2807)
                     /locus_tag="VFQ61_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955218.1"
                     /translation="MAKDDKKNEKVKVVIDIEDLRDIASESAEREDLAGKGAGARASA
                     QIKQAGDTIMCPW"
     gene            3323..5458
                     /locus_tag="VFQ61_11980"
     CDS             3323..5458
                     /locus_tag="VFQ61_11980"
                     /inference="COORDINATES: protein motif:HMM:NF023417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0009820 - alkaloid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan dimethylallyltransferase family
                     protein"
                     /protein_id="HET9955219.1"
                     /translation="MQTMLDFGTRQLESLIDATGLSLDRAVARRTFEDLASSWRDRPV
                     ESVPRWSGLTDDCTPIEYSIVLGGRQPELRVSVEAQADPASPFTYWASGQRLCERLES
                     SYGAELAVLRRIEDLFRPSDPAAYTAMYHGAVFRPSGKPLFKVYLSPPAQGAEYANQA
                     VEKACERLGLLGSWRRIAATLRPRDSLIGLSVDVVPELGARLKLYVRHREPTLQDLER
                     ASSLADEYVPGDVTRFVAAMLGVSAEQLPSGVVLRRPPVSTYNLSAGQHGSEPHGSEQ
                     HGAVPRVTFHIPVFPFCVENDGMARDRIVKLLHDHRLSSDVYERCLGALANRDLYAEQ
                     GIHSYVSFQREDGHARITTYFCPRMYRHTYGPLALDPGFPWPSPVKAERPAKQMPAAP
                     AEPKRERAPEDTAPADDSSAPAVVLNRAARVRAIRRDGNIVGYQVAAPRRNVGLIDTR
                     VLADDSPLTWRALEQVLRPHHGAEVELNDEQLKQLLELGLLVRSQEHPARVTFDTGLL
                     GVMHEPAVATGHAGKDPLPRRLAQTRFAFERSGFGVVRGLLPPELLSALQTYYRGMVR
                     EGYLPHGDPQSKRYYCHNEEVAVRLHQLLLPIAQQIVPEPIKPSYSYFACYTEGAELA
                     RHTDREQCEVTLSLAIDATPQNTQDSAWPLNIERADGSIEAVLLAPGDGAFLRGRVLP
                     HFRAPLPAGRTSTSVFFHFVPAEFSGSLD"
     gene            complement(5653..6861)
                     /locus_tag="VFQ61_11985"
     CDS             complement(5653..6861)
                     /locus_tag="VFQ61_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955220.1"
                     /translation="MQAFQDLGQSIQALWKNERFELQAFARIASAALHGSQLHQRVKV
                     TDLLEWLRTTPCLPEQHDLAGKFAQPPLTVYRGDGFFIDALFWNEATTSIHQHRFSGA
                     FSVLSGSSLHTEYSFEQRERINQRLLLGDLRLRQIELLQEGDVRPIHSGSAFIHSVFH
                     LERPTVSVVVRTIRDPGTDPQYDYHRPGLAIDAHGEYPRRERLRQTFELLKNGIPAQY
                     LPFVREVALDDPELAYWALGHAYGRCTAEEWAELVSATAKRHGPAIHRFVESFEERAW
                     ERQLIARRANTTVADQRFFLAVLLNAPDRSTARDLIQARFPHQGADEALVDLTFGLHS
                     KSGSELSPATHEFVRLVLTGADFGDVMTQLDQRYASAEIRAQAPALESLHGSFRSSPL
                     FRRLFVDSAA"
     gene            complement(6898..7332)
                     /locus_tag="VFQ61_11990"
     CDS             complement(6898..7332)
                     /locus_tag="VFQ61_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955221.1"
                     /translation="MTLIGPPHPMADSDFSPDAQDSLFWASETIAVSRKKVLFRLLAN
                     QAAERLGLTPTAAEIQAESRRFRLKYGLAAPDNFRAWLARTALSLEQFSELMRDFVVV
                     AQLERRYASEVDAGLNHQQAVYTAHRWAAEHAPSPPHAPPER"
     gene            complement(7329..8834)
                     /locus_tag="VFQ61_11995"
     CDS             complement(7329..8834)
                     /locus_tag="VFQ61_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9955222.1"
                     /translation="MLLKKRLSDTVQLAVSYEDGTRYGSVLTGRCDQLIRQKLRHVAE
                     LRRERGLAHVVERSLEILTEAMRETSLGALYVRDGQLREACLYPEREAVRPASLLFFR
                     SPSEAQEFPLSPGLTAELATWCGEWQQGASAPQAPEARQLWDALDAAGLLTDEPPLPP
                     VEGEMVFGGHAMLRVGRTQRVLVDPFVLPRHSRYPASYQPFTYSELAPTAICITHSHP
                     DHFDVGSLLRFGPDIPIFVPAVPRESILAVDMAYRLRELGFRDVRPLPWFSEADLGSA
                     RISSLPFYGEQPTSGESLHPEVRNMGNLYVIECEGRRCAVTADAGWDRDGSTADLALL
                     SRRKQGALDVLFGGYRSWAMYPIQYLFCSVTSYLLFVPRDQWHVRQRIMNGLEELFDT
                     AERWQARHVVPYADGGAPWYWERGLGPRLDGAEAATSGRGRPNRHFDPDPEDAIALQG
                     MRSTAADGSPIASPTRVQLMRPGQALDFSATPLRQFDTPGHVWPYGSEARP"
     gene            complement(8824..>9107)
                     /locus_tag="VFQ61_12000"
     CDS             complement(8824..>9107)
                     /locus_tag="VFQ61_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955223.1"
                     /translation="FAIVEGRHPYANFFAHVAGKGLLRRLNGEAQPEALYSCGDLRLF
                     EKIIGVRDGRVYVPPVQGWELFECLPEPLTYFLRYPRKWGADLEAAGAA"
BASE COUNT         1716 a         2887 c         2905 g         1599 t
ORIGIN      
        1 gcacgaattg ggtgatgtag cggcgtccat gatcatcctc gagaaaagcc tgatccgctc
       61 gttcttccac gaccagcgcg ctgacgtggc tggccccctc gaacagggcc tggagtgcaa
      121 tggggcagtc gcgaggtacc agcagcgggg tctcgaaatc gcgcgcaacg tgcagcatgg
      181 cggcccagcc gtgtcgacgc gccaacttct gccaacgcac gaagcgcaat tcgggagagg
      241 cctcgattaa ctgcgtgaga tccgcgccgg agacgaccgt tcgtcggtcc cggatcacgt
      301 gtccttgcgg cgttttgatc tccggcgaag gacgccccga ctcgagctcg atggagaagg
      361 acatgctttc ggcacgcaag tgggcgagcc gccccgcgcg gaaccccgtg aacaacaagg
      421 cttggcacac cggatctcga taacccaaat tggcagacgc gaaagacagg accgccgcgg
      481 gttgggtttg cggagcagta ccaagaagga gtgttcgaga gcggctatct acgaacgttt
      541 cagcgcggaa caagccgagt tcgccggcaa ctgcgcccca cacggagaca cactgttcgg
      601 tatatcgcgg acgaaccagt gcgttcgggt tggcttcgca gggattcagg agctcacaca
      661 gcacgagccc cgtgcggtgc gagatctccg ccaactgcag cgtgaaccag ccgtagaacg
      721 ggtcgcgcgg cgcgcgggtg ataggattcc aaagcggcac gtagcgcgag tagatcgcgg
      781 ccacctcatc ggtggcgccc tgaatcacca cgcgcgagcc gtttagcccg aagatgagcg
      841 aagcgagcgg cgacacggcg gtttcggtgg cgcggttgcc atccaaagtc acgggctggc
      901 cctgtagcag ctcactccag ctctgcagcc gttgccggta ggcctcggga gcacgcatca
      961 ggcgctcgtg ggactcccac gtcaacagcg gctccgtggg agagggttcc gggacgagcg
     1021 gctcgaccgc gctcaacggc cgtcgtaaga gtgcgccatc cacgtactca tcgacgagcg
     1081 cgctgcgcag cgtgtagccg agcccgtact tacgttgaaa ctgctcgtat tcagcgatcg
     1141 cggtcgcaat tgctcgttgc gcctccacgc cgagcgtgac gcggaagggt aaacctgcgt
     1201 cgcatcggag cacgacgcgc ggcagttcgg gacaaccgag attcagatcg gccgcgagct
     1261 cgcggatcac ctgcgccgct cgatcgaagc gtagcagcag ctcactcggg gcgagcgcgt
     1321 cgagctcgcg ctcgagcttg cggcagaaac gccgaagctt gcggtaggcg acccaccacg
     1381 cagcgcgttc tgggccgtgc agcgcgcatg cggcctgagc caatgcttcc cagctcgagg
     1441 aataacgcaa tggcaggtcc aagccgccaa ccaaaatgcc cccgtcgatg caggccagca
     1501 gcactgcctc gacggtatcg acatctagac cagagtctgc accggcacgc tcgcataggt
     1561 cgccgaagga ggcggctggc acccctcgaa tcgttttcag aacctgcgcg acggcggcgg
     1621 gcgggcgcat gcgtttgcgg ctcacctggc cttgcgcgtt gcgggcccaa aaagtgaagt
     1681 agccttccac gtcccgctcc agggtgacgt tgaccttcca gagcccgact ttgcggtagt
     1741 cctcgcgcgc ggcaagggcg cgcaacagac tttggaagac acgcagatct ggtgcgaagc
     1801 gcacgggtgc gggcgcagct tcgactctcg tgctctctcc aaaattcgcc aaggtcacgc
     1861 cggcccacaa ctcacacggt tcgacccggc ccgcggcgcg ggccaagtag cggtataacg
     1921 tggtctgcag gtggcgagcg cgcttgtttt gcttgaggat tccctcggcg gccagagccc
     1981 gctgcgcgag atccgggttg gaaaccacca gcgcacgcat gaagctcgga tcctgtgccg
     2041 tcagacgcca caacgttctg agttgctcct ggcgctcatg atcgaatcga accacatact
     2101 cgctccaagc cgcaggatcg ctctcgtcga gttgaccggc caactcggcc agctccggcg
     2161 cggcgagcgc ttccaacgtc tgcaccggcc agcgcgcggc ccgcgtaaac acctgagggg
     2221 cgtgtcgaat cggcggcact tggctcggct cggtaccgat acagggagac tcagccaccg
     2281 cgagacgcgg cgctactttc aactcgtggt cggagccggt ggaatcgagg gttgcggggc
     2341 gcacggctgg aagcggcatc acgagttctg ctcgatctag tttctggagc tcagtgcgag
     2401 tctgcaattc ggtgtaatcg gtaattgact gtaattggcg agacaccggg attcgggagc
     2461 atgggattgc tggcacgaca gcaggcgcgc gggcccacgc gacgaatcga agttcttggc
     2521 ttcgttaccg tcgcgccagc tcatttgaac gactcactgg gtgaccgagg cgatcaccca
     2581 gcttctcgaa ggtgagagcg cctagcggga gtggctccag ccaggacgcc gtctcaccag
     2641 ggacacatga tcgtgtcgcc cgcctgcttg atctgggccg aagcacgggc gcccgcgccc
     2701 ttgccggcta gatcttcacg ctcggcgctc tcgctggcga tgtcgcggag atcctcgatg
     2761 tcgatcacaa ctttgacttt ttcgttcttc ttgtcgtctt tggccatggg gttccttcag
     2821 gggttttagc cctgtattcg aaagccaccg cgtgagcgcg tagctccgca cccggttgga
     2881 cgcagagcat gctccacgcg aatggcgaca cactggctcg ccgcaccagc gcagcgagtc
     2941 agctgagtga ggaccgtaca cgatcgaaaa atgaattcca tggttgtcag ttttggtgtt
     3001 tcacaaaatt cgttgaggcc gactccggtg ttgtgaagtt tctcaattgc gattccgaac
     3061 ctctatgagg aactcctacc gttctcggcg cttcgctctc cgatggcccg ccgggccgcg
     3121 tggatcgaga tggggcgtac gagtgtccgg caggcgagcg cgttttgggc cgaactctcc
     3181 ggcaaattgc tactgactag ctttcacgag cccgctatca cggagcagtc gctcgcgtga
     3241 gcggacgcca tgttcggtag ccgcttatga gcccgtcagc gtgccctcac gccctgaact
     3301 caactcacac agagaactcg ccatgcagac catgttggat tttggcaccc ggcagcttga
     3361 gtcattgatt gacgccacag ggctctcgct cgatcgggcg gtcgcgcgtc gtaccttcga
     3421 agatttggcc tcttcctggc gagatcggcc ggtcgagagt gttccgcgtt ggagtggact
     3481 tacagacgat tgcactccaa tcgagtattc gattgtgctg ggcggaaggc agcccgagtt
     3541 gcgggtgtcg gtcgaggcgc aggccgaccc cgcgtctccg ttcacttact gggcttcggg
     3601 tcaaaggctc tgcgaaagac tggaaagctc ctatggtgcg gagctcgcgg tgctgcgtcg
     3661 aatcgaagat ctattccgtc cgagcgaccc cgctgcgtat accgcgatgt accacggagc
     3721 ggtgtttaga ccgtccggta agccgctctt caaagtctat ttgagccctc ccgcgcaagg
     3781 cgcggagtac gccaatcagg cggtcgagaa ggcttgtgag cgacttggac tgctgggctc
     3841 ctggcggcgc atcgcggcca cgcttcggcc tcgagactca ctgattgggc tttcggtcga
     3901 tgtggtgccc gagttgggtg cgcgcctgaa attgtacgtg cggcatcgtg aacccacgct
     3961 gcaggatctc gagcgggctt cgagcctggc cgacgagtac gtacccggcg atgtcacccg
     4021 cttcgtggcc gccatgctgg gcgtctccgc cgagcaactg ccgagtgggg tcgtgcttcg
     4081 gcggccgccc gttagcactt acaacctcag cgcggggcag catggctctg aaccgcatgg
     4141 ctctgaacag cacggcgcgg taccgcgcgt gaccttccat atcccggtat tcccattctg
     4201 cgtcgaaaac gacggcatgg cgcgggaccg catcgtcaaa ctcttgcacg accatcgcct
     4261 gagcagtgac gtttacgagc gttgcttggg cgctctcgcc aatagggact tatatgcaga
     4321 gcaaggcatt cactcttacg tctcgttcca gcgcgaggac ggccacgcgc gcatcaccac
     4381 ctatttctgc ccgcgcatgt accgccacac ctacggccct ctggccctgg atccaggctt
     4441 tccgtggcct tcgccggtga aggcagaaag gccggccaag caaatgccgg cggccccagc
     4501 cgaacccaag cgcgagcgcg cacccgaaga caccgccccg gcggatgatt cgagcgcgcc
     4561 cgcggtcgtc ttgaaccgcg ccgctcgcgt gcgcgccatt cgtcgggacg ggaatatcgt
     4621 cggttatcaa gtggcggcac ctcgtcgcaa cgttggactg atcgacacgc gggtgctcgc
     4681 cgatgatagc cctctcacct ggcgagcgct cgaacaggtg ctgcggcctc atcacggcgc
     4741 agaggtcgaa ttgaacgacg agcaactcaa acaactgctg gagctcgggt tgctcgttcg
     4801 gagccaagag caccccgcgc gggtgacctt cgatactggc ctactcggtg tgatgcacga
     4861 accggctgtg gccacgggcc atgctgggaa agacccattg ccgcgccgcc tagcgcagac
     4921 ccgtttcgcc ttcgagcgga gcggcttcgg ggtggtgcgt ggcttgctac caccggaact
     4981 gttgagcgcg ctgcaaacct actatcgcgg gatggtgcga gagggatact tgccgcacgg
     5041 cgacccgcaa tccaagcgct actactgcca caacgaggaa gtcgcggttc ggctccacca
     5101 gctgctgttg cccatcgctc agcagatcgt gcccgagcct atcaagccct cgtattcata
     5161 cttcgcctgc tatacggagg gagccgaatt ggcccgccac accgaccgag aacagtgtga
     5221 ggttacgctc tcgctcgcga tcgacgccac gcctcagaac acccaagaca gcgcctggcc
     5281 gctcaacata gaacgtgcag acggcagcat cgaagccgtc ttgctcgcgc ccggcgatgg
     5341 cgcatttctc agaggccgcg tgcttcccca cttccgcgcg cccttaccgg cgggcagaac
     5401 cagcacctcc gtcttcttcc acttcgttcc tgccgagttc agcggcagct tggattgagg
     5461 tctgagtcgt tcgcgagtgg gtgagccatc gaggggggct cgcctgctcg cgagtagcac
     5521 tggttttgct ggccaccgag tcaacgggcg cgccgtgcgg cccaacacgt cgccagcgca
     5581 gccgaagcgc tcacaccgca cgtccgtcac cgcgcaggcc cccttcagtg cgaactcacg
     5641 agagctctga atctaagccg ccgaatccac gaacagacgg cgaaataacg gcgaacttcg
     5701 aaacgagccg tgcagggact cgagtgccgg cgcctgggcg cggatctcgg ccgaggcata
     5761 acgctgatcg agctgcgtca tgacgtcccc aaagtccgca cccgtgagca ccaagcgcac
     5821 gaactcatgc gttgcgggcg agagttcgga gccggacttg gaatgcaaac caaaggtcag
     5881 atcgaccagc gcttcgtccg cgccttggtg cgggaagcgc gcctgaatca ggtcgcgcgc
     5941 ggtcgatcga tcgggcgcgt tcaagagtac cgccaagaag aagcgttggt cggccacggt
     6001 ggtgttggcc cggcgcgcaa tcaactggcg ctcccaggcc cgctcttcga agctctcgac
     6061 gaagcggtga atggcggggc cgtggcgctt cgcggtcgca ctcacgagct ccgcccactc
     6121 ctcggccgtg caccggccat acgcgtgacc gagcgcccag taggcaagct cgggatcatc
     6181 gagcgcgacc tcacgcacga agggcaggta ctgagccgga atcccgttct tcagaagttc
     6241 gaaggtttgg cgtagccgct cgcgtcgcgg gtattcgccg tgtgcgtcga tggcgagacc
     6301 ggggcgatga tagtcgtatt gcggatcggt gcccggatct cggatcgtgc gaacgaccac
     6361 ggaaaccgtc ggtcgttcca agtggaacac cgaatgaatg aacgcactgc cggagtgaat
     6421 cgggcgaacg tctccctctt gcagcagctc gatttggcgg agcctcaggt cgcccagaag
     6481 caagcgttga ttgatgcgct cgcgctgctc gaagctgtat tcggtgtgca ggctcgagcc
     6541 ggacagtacc gagaaggctc cactgaagcg gtgttgatgg atcgatgtcg tcgcctcgtt
     6601 ccagaacaag gcgtcaatga agaagccgtc ccctcgatac acggtgagcg ggggttgggc
     6661 gaacttgccc gcgagatcgt gctgttcggg taaacacggc gtggtgcgca accactcgag
     6721 caggtccgta actttgactc gctggtgcag ctggctccca tgcaacgccg cgctcgcgat
     6781 gcgcgcaaat gcctggagct cgaagcgctc gttcttccac agtgcttgaa tcgactgacc
     6841 caaatcctga aatgcttgca tgacagcgcg tgaacctggt caacggagaa tgggctctca
     6901 gcgttccgga ggtgcgtgcg ggggggaggg cgcgtgttcc gcggcccagc ggtgcgcggt
     6961 atagacggcc tgttgatggt ttagcccggc gtccacctcg gacgcatagc gacgctcgag
     7021 ctgggccacc accacgaagt cgcgcatcaa ttcgctgaac tgttcgagcg agagcgcggt
     7081 gcgagcgagc caggcccgaa agttatccgg cgcggcgagc ccgtacttga gtcgaaagcg
     7141 ccgggattcc gcctgaatct ccgccgcggt gggggtcaag ccgagccgct ccgcggcttg
     7201 attggccagc aggcgaaaga gcaccttttt gcgactgacc gcgatagtct cgctcgccca
     7261 aaacaacgaa tcctgagcgt ccggggagaa gtcggagtcg gccatcgggt gcggtggtcc
     7321 gatcagcgtc atggacgagc ctcgcttccg taaggccaga cgtgccccgg ggtatcgaac
     7381 tggcggaggg gggtggcgct gaagtccaga gcctgcccag gacgcatcag ctgcacgcgg
     7441 gtcggggacg caattgggct gccgtcggcc gcggtcgagc gcatgccctg aagcgcgatg
     7501 gcgtcttcgg gatccggatc gaagtgacga ttgggacgac cgcgacccga agttgcggct
     7561 tccgcgccat ccaagcgcgg gccaagtcca cgctcccaat accaaggggc tcccccgtct
     7621 gcgtacggca cgacatgccg agcctgccag cgttcggccg tatcgaacag ttcttcgagc
     7681 ccgttcatga tgcgctgacg cacgtgccac tggtcccggg ggacgaacag caaatagctc
     7741 gtcacggaac agaacaagta ctgaatcggg tacatggccc aggagcgata tccgccgaac
     7801 agcacatcca acgcgccttg ttttctgcgg gagagtaggg ccaagtcggc ggtgctcccg
     7861 tcgcgatccc agcccgcgtc cgccgtgacc gcgcagcgtc gcccttcgca ctcgatcacg
     7921 tagaggttgc ccatatttcg gacctccggg tgcaggcttt ctcccgaggt gggttgctcg
     7981 ccgtagaacg gcaggctcga gatgcgcgcc gagccgagat ccgcctccga gaaccacggc
     8041 agcggccgaa cgtcgcgaaa gccgagctcg cgcagacgat aggccatatc cacggccaag
     8101 atggattcac gcgggaccgc gggcacgaag atcgggatat cgggaccgaa gcgcagcagg
     8161 ctcccgacgt cgaaatggtc ggggtggctg tgcgtgatac aaatggccgt tggcgcgagc
     8221 tccgagtacg taaacggctg ataagaggct gggtagcgcg aatggcgcgg caaaacgaag
     8281 ggatccacga ggacccgttg ggtgcgcccg acgcgcagca tggcgtgccc accgaacacc
     8341 atctcacctt ccaccggggg caacggtggt tcgtcggtga gtagcccggc ggcgtcgagc
     8401 gcgtcccaca gctggcgtgc ctcgggcgct tgcggcgcag aggcaccctg ctgccattct
     8461 ccacaccagg tggcaagttc cgcggtcagg cccgggctga gcggaaactc ctgcgcctcg
     8521 gacgggctgc gaaagaacaa caacgaagcc ggtctcacgg cttcgcgctc aggatacaaa
     8581 caggcttctc gcagttgccc atcacgcacg tacagcgcgc ccaagctcgt ctcgcgcatg
     8641 gcctcggtga gaatctcgag cgaacgctcg accacgtggg ccaagccacg ctcgcggcgt
     8701 agctcggcca cgtgtcgcag cttctgacgg atgagctggt cgcagcgccc cgtcagaaca
     8761 ctgccatagc gcgtgccgtc ctcataagag acggccaact gcaccgtgtc ggagaggcgc
     8821 ttcttaagca gcaccggcgg cctccagatc cgccccccac ttgcgcggat aacgcaggaa
     8881 gtacgtcagt ggctccggca agcactcgaa tagctcccag ccctgaaccg gaggcacgta
     8941 gacgcgccca tcgcgcacgc cgatgatctt ctcgaacagc cgcaaatcac cgcaggaata
     9001 gagggcctcg ggttgggcct ctccattcaa gcgtcgcagc agccctttgc ctgccacatg
     9061 cgcgaagaag ttcgcgtacg gatggcgccc ttccacgatc gcaaagc
//
LOCUS       DASTKU010000342         8523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_66182,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000342 DASTKU010000000
VERSION     DASTKU010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8523
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66182"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..682)
                     /locus_tag="VFQ61_12005"
     CDS             complement(155..682)
                     /locus_tag="VFQ61_12005"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HET9955224.1"
                     /translation="MTRAALPVSSPSLTAGSPAPDNELWFAALERRLTAEAQSQRPTI
                     PERLTVLFDPSCALCRRARAWMLGQPTYVELEFLPANSADANHRYGALPWLGDELVVV
                     SNHGQVWVGPAAFITCLWALVEWRPWSYRLAGSFSGLAERFFLTISKNRKVLSGMIEH
                     ECEDGHCRIGASRAR"
     gene            complement(679..1161)
                     /locus_tag="VFQ61_12010"
     CDS             complement(679..1161)
                     /locus_tag="VFQ61_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955225.1"
                     /translation="MADTSRFLLLAYFIYAVASFALTVFLARTLFKNGEVFLEDVFPD
                     NSRMAASVNRLLVVGFYLLNLGYAFLTLQSHETVNSALVAFEVLSSKLGQLLVTLGIV
                     HFGNLFILHRMRRRGQIHLAPPPVAPQLWRPQAPIGNPGASAAAALANGPSGSMPGAI
                     "
     gene            complement(1280..2812)
                     /locus_tag="VFQ61_12015"
     CDS             complement(1280..2812)
                     /locus_tag="VFQ61_12015"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9955226.1"
                     /translation="MSSACSRCGLVCPGLGIALCPRCLLSADAEDGDSEDDVSDVDNA
                     SLRAAALEVPGLRLARVLGRGAMGQVWLARHVRLDRDVAVKVIAPELALDAAFVARFE
                     REARALARLNHPHVVSIYDFGVTEQGMLYLVMEYAPGGTLSERIPMRPAAAVEVLLVL
                     CDAVFSAHQSGLVHRDIKPHNVLFDAHGRAKVADFGLARGFELDATDGLTRSGLVVGT
                     PPYMAPEARAGAPPHPAADQYALGVLLRELVTGTPAPSDRPLPEALRGIVRRATAEAA
                     TDRYADVKSLQTALRGVLSTLGETKERAAGDRRSTHIGLPPEERSWLQAVSLTFAGAN
                     ALALYAFLTSFTPRVLPSEDTLPMIAFGVRALPDGRVFSRARFETWPTLAAGAGFAIA
                     LAAYGLLRRHWRIAGLEQPQPDAEIESGTRALQLGIVLFALFIAHRVLALTPLRAVSP
                     FLPVLGGTLEFLLLYLVWHSLLEAVRCQRSWLKEYRVWLGLLLGLIPPGFHFFELLLK
                     SP"
     gene            complement(2809..3531)
                     /locus_tag="VFQ61_12020"
     CDS             complement(2809..3531)
                     /locus_tag="VFQ61_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9955227.1"
                     /translation="MPEQDPSPGRADVFPATRWTLILRAAESPEARRVALESLVAPRW
                     RALYAMARKKGFSKERAEDAVQSFVERLASGDALARLDPSRGKLRAYLSAAFSKHLLN
                     VHVHEAALKRGAGRRSVDLDAIEASVPAWGDTPEEVFDRAYAIALYQSALEDLRAEFA
                     AGDRTGPFEILERLFRFGETESYAELAPRYGLTVPQLKSLVHRAKRRFQQLFRTRIAE
                     TVDDAADVDAELGAVLSLLAGK"
     gene            complement(3620..4960)
                     /locus_tag="VFQ61_12025"
     CDS             complement(3620..4960)
                     /locus_tag="VFQ61_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9955228.1"
                     /translation="MTRRSDPPDVPPEPVYVIEAIPVPGEHEVSREEIRMEGIPTPPH
                     LRIFAELGRGAMGRIHPATDRNLLRHVALKRLDRELAEQPFYRDGFVAEAQITGQLEH
                     PNIVPVHELSIDPNGVPYFTMKLVEGESFARWLNHASRPPGSTDRIDPALDVLLKVCD
                     AVAYAHHRGVLHRDLKPENVMVGSFGQVYLMDWGLSRLTKTRPASGASAQMEAPGPVG
                     TPEYMAPEQARGNPAEMTERSDVFGLGAILYEIVSGKRPYGEAPNQDVVLERARQGAV
                     VPIGVATAGLFVSKRLLRIVEKATAADPAQRYESPTALGRDIQDFLRGELHLPRKFFL
                     PGACIIREGDVGDAAYMIVSGTCRAFRQVGDKVEPLATMNAGDVFGEMALLMDEPRAA
                     SVEAVSAVTVLVLDKATLAEGLGLTGWTAALVHALAQRFRDLERQVRNSGMLRS"
     gene            complement(4967..7420)
                     /gene="pheT"
                     /locus_tag="VFQ61_12030"
     CDS             complement(4967..7420)
                     /gene="pheT"
                     /locus_tag="VFQ61_12030"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET9955229.1"
                     /translation="MKVPFSWLMQYFASPLDPLEVAERLTLSGIEVEEVRGVGALDPN
                     VVVGEVREVSALPGGAGFRLVVDTGGTRSIVTRAEGVSVGQKLAVALPGATLLSSNLE
                     QLEDVSESEVRGVRSAGMLASAATLGIGSDAEHAFLFGADAPIGAPVSQVFSATKSEA
                     DWVLLLAILPNIARCQSMLGVAREVAALLAQPLTPPAPIAAIRISHEGPSKLAPQIQA
                     PDVGRVLRLTLIENVQVTESPRWLKRRLLLAGMTPINNVVDASNYVMLELGEPSHPYD
                     ADLLPSCDLAVRRARAGERLLTLQQAEGEEPMALPEGVPLIVSADRPVAIAGVIGGRE
                     SAISTRTTRVLLEAAAFDYVAIRRSQQAAKVYSEASARFSRGVNPELPALASARFIEI
                     LRETSPGLRVVDQNEVSLGIPEPRRLELSLSELNDSLGTQLDLDAAYECLSRIGLEVL
                     QEPAAARLIVNVGNDRADITVPRDLVEEVARLAGYDRIPETLPKEPIPERRHDDWVSR
                     REALRDLLVRLGLQEILSYSLTSPEVEARLGAGHPGIELPAHVGLLNPVSVERSVLRS
                     SLFGGLLQAMAVNLKHSPLCRMFEIGPAFHPRASARELPTEELRVTALLGGLASEPGL
                     QDAKPRALDFFDAKAVLEAMFAGLGLPGIELEVGDAAPYRPGAVARVLRGGRSYGWIG
                     AIHPKVLSAFDLAGRPVFAFDLDLEALLRDAPPRRSFSDYDRLPSIELDVAMILDRAI
                     QAGRIRSVARQAAGELLRDVEVFDQFFSAEFGEDRKAIAIRLRLNAGQRTLEMAEAVQ
                     VRERVARALESELSARIRE"
     gene            complement(7547..>8523)
                     /gene="pheS"
                     /locus_tag="VFQ61_12035"
     CDS             complement(7547..>8523)
                     /gene="pheS"
                     /locus_tag="VFQ61_12035"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET9955230.1"
                     /translation="ELSDEKALEGWYRKHLAPSGTMNQWRKRIGAEPVEQRKAFGAKV
                     NAAASALDAAFRGQKQRISELELSRAIQRESIDVTLPSRSVVTGGYHPITTAIRQVEK
                     VFHEMGFTTFESPHAELDEFNFELLNMPADHPARDMQDTFYLSDRTVLRTHTSAGQVR
                     AMRLYAPEPVRVILPGLCYRHENITTRSEIQFHQIEGLLVGKTVRLSDLKGVLLRFAR
                     SMFGRDQQIRLRGSYFPFTEPSVEVDIRCTLCGGKGCRVCKNSGWLELLGAGLVHPVV
                     LKNGGYDPNTVRGIAFGMGIERMVMLLQGIDDIRHFYQNDLRFLRQFR"
BASE COUNT         1645 a         2862 c         2654 g         1362 t
ORIGIN      
        1 atgtggccca cgacgtgcga ttcaaccctc cgagcacggg aagtgtcgaa atccagattc
       61 cggtgcactt cgtagagatg cccccggagc ccacgccacc gaagtccgca agcggaagca
      121 aagccacagg caatgtctcg ccccgtaggt agcttcatcg cgctcgcgat gccccaattc
      181 gacagtgacc atcctcacat tcgtgctcga tcatgccgct caagaccttt cgattcttcg
      241 agatggttag gaagaagcgc tccgcgagcc ccgagaacga acccgcgagt cgatacgacc
      301 aggggcgcca ttcgacgagc gcccataagc acgtgatgaa cgccgccggc ccgacccaga
      361 cttgcccgtg gttggacacg acgaccagct cgtctccaag ccagggcaac gcaccgtagc
      421 gatggttggc atcggctgag ttcgcgggta agaactcgag ctctacgtac gtgggctgcc
      481 ccagcatcca agcccgagct ctacggcaca gcgcgcagct cggatcgaac agcaccgtca
      541 aacgctccgg gatcgtcggt cgttgggact gggcctcggc ggtcagccgg cgttcgagcg
      601 cggcgaacca aagctcgtta tccggcgcgg gcgagcccgc cgtcagagac ggcgagctga
      661 ctggaagtgc cgctcgggtc atatggcccc cggcatcgat ccagaaggac cgttcgccaa
      721 agctgcggca gcgctagctc caggattgcc gatcggcgct tgaggacgcc aaagctgcgg
      781 ggcgaccgga ggcggcgcca ggtgaatctg cccgcgccga cgcatgcggt gcaggataaa
      841 caggttcccg aagtgcacga tgccgagcgt gaccaacagt tggccgagtt tgctcgataa
      901 tacctcgaac gcgacgagcg ccgagttgac cgtctcgtga gactgaagag taagaaacgc
      961 ataccccagg ttcagcagat agaagcccac caccagtaag cgattgacgg atgcggccat
     1021 gcgcgagttg tctggaaaca cgtcttctag aaagacctcg ccgttcttga acagggtccg
     1081 cgccagaaac acggtcagcg caaagcttgc gacggcgtaa atgaaatagg ccagcaacag
     1141 gaagcgagat gtatccgcca tggagagttc ctttcgggtt cacctccctc tacagagctc
     1201 tttgccagcg gttgcgtgtc ctcgtcgatt ttccgttcag ggctcgattc ccgatactct
     1261 gggcacactc cgggcgcttt cagggcgatt tgagcagcag ctcgaagaag tgaaagccgg
     1321 gcgggatcag cccgagcagc agcccaagcc aaactcgata ttccttgagc caggagcgtt
     1381 gacagcggac ggcttcgagc aggctgtgcc acaccaagta gagcagtaga aactccaagg
     1441 tcccaccaag cacaggcaaa aacgggctca cggcccgcag cggcgtgagc gcgagcaccc
     1501 ggtgggcgat gaacagcgcg aacagcacga tacccaactg caaggcgcgc gttccactct
     1561 cgatttctgc atcgggctgc ggctgctcga gccccgcgat ccgccagtga cggcggagaa
     1621 ggccataggc ggcaagcgca atcgcgaagc cagcccccgc cgccaacgtc ggccaggtct
     1681 cgaagcgagc gcgcgagaac acccgcccat caggtaaggc gcggaccccg aacgcgatca
     1741 tgggcagtgt gtcctcgctc ggcagtaccc gcggagtgaa cgacgttaga aacgcgtaaa
     1801 gcgccagcgc gttagcgcca gcaaacgtca agctgaccgc ctgtagccag ctccgctctt
     1861 cgggaggcaa accgatatgc gtgcttcgcc ggtcacctgc tgcgcgctct ttcgtttctc
     1921 ccaaagttga gagcacgccc cggagcgccg tctgcaaaga cttcacgtcc gcatagcgat
     1981 cggtcgcggc ttcagcggtg gcgcgtcgta cgattccccg caacgcctcc gggagcggtc
     2041 ggtccgacgg cgcgggcgtc cccgtgacca actcgcgcag gagcacgccg agcgcatact
     2101 gatcagcggc cgggtgaggc ggcgcacccg cgcgcgcctc gggggccatg tatggcgggg
     2161 ttcccaccac caaacccgac ctcgtcagac cgtcggtggc gtcgagctcg aagcccctgg
     2221 ccaagccgaa gtcagcaact ttggcgcgac catgagcatc gaacaagacg ttgtggggct
     2281 tgatatcgcg atgcacgagc ccgctctggt gcgccgagaa gaccgcatcg cacagcacca
     2341 acagcacctc gaccgcggcc gccggacgca tcggtatccg ttccgacaac gtgccccccg
     2401 gcgcgtactc catcacgaga tagagcatgc cctgctccgt cacgccaaaa tcgtaaatgc
     2461 tcacgacgtg gggatgattc agtcgagcca acgcgcgggc ttcgcgctcg aaccgcgcca
     2521 cgaacgccgc gtcgagcgcc agctctgggg caattacctt cacggccacg tcgcgatcga
     2581 gccgcacgtg acgcgcgagc cagacctggc ccatcgcccc ccgccccagc acgcgcgcca
     2641 gacgtagccc gggcacttcg agcgctgccg cgcgcaacga tgcgttatcg acgtcgctca
     2701 cgtcatcctc cgaatcacca tcctcggcgt cggcactcaa caggcaacgc ggacagaggg
     2761 caatcccgag ccccggacaa accagcccac agcgtgaaca agcgctgctc atttgccggc
     2821 cagcaaagac agcaccgcgc cgagctctgc gtccacgtcg gccgcgtcat ctaccgtctc
     2881 cgcgatgcga gttcggaaga gctgttggaa gcggcgttta gcgcggtgaa ccaacgactt
     2941 gagttgcggg accgtgaggc cgtagcgagg cgcgagctcc gcgtaagatt cggtttctcc
     3001 gaatcggaat agtcgctcca gaatttcgaa cggcccggtg cggtcgcccg cggcgaattc
     3061 agcgcgcaga tcctcgagcg cgctctggta caaggcgatc gcgtacgcgc gatcgaagac
     3121 ttcttccggc gtatcacccc acgccggtac gctggcttca atcgcgtcga gatcgacgga
     3181 gcgccggccc gcgccacgtt tgagcgcagc ctcgtggacg tgcacgttga gcagatgctt
     3241 ggagaaggcg gcgctgagat aagcgcgcag cttgccgcgg ctcggatcca accgcgcgag
     3301 agcgtcaccg ctggccaagc gttccacgaa gctctggacg gcgtcctcgg ctcgctcttt
     3361 cgagaacccc ttcttgcgag ccatggcgta cagcgcacgc cagcgcggcg ccacgagcga
     3421 ttcgagcgcg acgcggcgtg cttctgggct ttccgcggcc cgcaagatca aggtccagcg
     3481 tgtagccgga aagacatcgg cgcggccggg gctggggtcc tgctcgggca tcccgcgcat
     3541 tataatgtga gccccgccgt tggcgcgcca agcttctcga gccctccccg tggggcccag
     3601 ccacgggcgc ttatcgcgct cagctccgta gcatgccgga attgcggact tgtcgttcca
     3661 ggtcccggaa gcgctgagcc agcgcgtgaa ccagagcggc agtccagccc gtgagcccca
     3721 ggccctcggc gagcgtcgct ttgtccagca cgagcacggt cacggcactg acggcctcga
     3781 cgctggccgc gcgcggctcg tccatgagta acgccatctc gccgaacaca tcccctgcgt
     3841 tcatggtcgc gagcggctcg accttgtctc caacctggcg aaacgcgcgg cacgtgccgc
     3901 tcacgatcat gtaagcggcg tcacccacgt caccctcgcg gatgatgcaa gcaccgggca
     3961 gaaagaactt gcgcggcagg tgcagctcac cccgcaagaa gtcttgaata tctcgaccca
     4021 gcgccgtggg gctttcgtag cgctgcgccg gatcggcagc cgtcgctttc tcgacgattc
     4081 ggagcaggcg cttggacacg aacaagcccg cggtagcgac gccgatcggg accactgcac
     4141 cttggcgggc gcgctccaag accacgtctt gattcggggc ctcgccgtac gggcgcttgc
     4201 cgctcacgat ctcgtagaga atcgctccca acccgaacac gtccgagcgc tccgtcatct
     4261 cggcgggatt gccccgcgcc tgctcgggcg ccatgtattc gggtgtcccg acgggcccgg
     4321 gcgcttccat ctgcgcggat gcgccggaag caggccgagt cttggtcaga cgggagaggc
     4381 cccaatccat caagtaaacc tgcccgaagc taccgaccat cacgttctcg ggcttcaaat
     4441 cgcggtgcag cactccacga tggtgcgcgt acgcgacggc gtcacacacc ttgagcagta
     4501 catcgagcgc gggatcgatg cgatcggtcg aaccgggcgg acgtgacgca tgattcagcc
     4561 agcgcgcgaa gctctctcct tccacgagct tcatcgtgaa gtaaggcacc ccattcggat
     4621 cgatggacaa ctcgtgcacc gggacgatgt tggggtgctc gagttgcccg gtgatctgcg
     4681 cttcggcaac gaaaccatcc cgataaaatg gctgctccgc cagctcccga tcgaggcgct
     4741 tcaacgcaac gtgtcgcagc agattacgat cggtcgcggg atgaatgcgc cccatcgcgc
     4801 cccgccccaa ttccgcgaag atccgcaggt ggggcggcgt aggaatgccc tccatccgga
     4861 tttcttcgcg gctgacctcg tgctcccccg gcaccgggat ggcttcgatc acgtaaaccg
     4921 gttcgggcgg cacatcgggc gggtcagatc tgcgtgtcat ggggcttcat tcacggattc
     4981 gcgcggagag ctcgctctcc agcgcgcgag ccacgcgctc gcgcacctgc acggcttccg
     5041 ccatttcgag cgtgcgctgg ccggcgttca aacgcaatcg aatcgcgatg gccttacggt
     5101 cctctccgaa ctcggcgctg aaaaactggt cgaaaacttc gacatcacgc aacaactccc
     5161 ccgcggcctg gcgtgccacc gagcgaatcc gtcccgcttg gatggctcgg tccaggatca
     5221 tcgccacgtc gagctcgatg gacggcaggc gatcgtaatc gctgaagctc cgccgcggcg
     5281 gtgcgtcgcg cagcagggcc tcgagatcga ggtcgaaagc gaatacaggg cgacccgcca
     5341 aatcgaacgc cgataatacc ttggggtgaa tagcaccaat ccaaccgtag ctgcggccgc
     5401 cgcgcaggac gcgcgccacc gcgccggggc gatacggcgc ggcatccccc acctcgagct
     5461 cgatgccggg cagcccgagc cccgcaaaca tggcttcgag cacggccttg gcgtcgaaga
     5521 agtcgagtgc acgcggcttg gcatcctgca agccgggttc gctcgccaaa ccgcccagca
     5581 acgcggtgac gcggagttct tcggtaggca gctcccgagc gctggcgcga gggtgaaacg
     5641 caggcccgat ctcgaacatg cggcacagcg ggctgtgttt gaggttcacg gccatcgcct
     5701 gcagcagtcc gccgaacagg gagctgcgca gcacagagcg ctcgacgctc accgggttga
     5761 gcagccccac gtgggccggc agctcgatcc cgggatgccc ggcccccaga cgcgcctcca
     5821 cctccggtga agtgagtgag tacgacaaga tctcctggag ccccaggcgc acgagcaagt
     5881 cgcgcaaggc ctcgcgccgc gaaacccaat catcgtggcg ccgctccgga atcggctcct
     5941 tgggcaaggt ctcgggaatg cgatcgtaac ccgcgagccg cgccacttct tcgaccagat
     6001 cgcgtggcac ggtgatgtcc gctcgatcat tgccgacgtt cacgatcaga cgcgccgccg
     6061 cgggttcttg gagaacctcg agcccaatcc tcgaaaggca ttcgtatgcc gcatccagat
     6121 ccagctgggt gcctagacta tcgttcaatt cggagagtga tagctcgagt cgacgcggct
     6181 ccgggatgcc cagcgagacc tcgttctgat ccacgacgcg cagcccgggc gaggtctccc
     6241 gcagaatctc gatgaagcgc gcagacgcca aggcgggcag ctcggggttc accccacggc
     6301 tgaagcgcgc cgaagcttcg ctataaacct tggccgcttg ctggctgcgc cggatcgcca
     6361 cgtagtcgaa cgcggcggcc tcgagcagca cgcgcgtagt ccgcgtcgaa atcgcgctct
     6421 cgcgcccgcc gatcacaccc gcaatcgcca ccggtcgatc ggcagaaacg atcaacggaa
     6481 cgccttcggg caacgccatg ggttcctctc cctccgcctg ctgcagcgtg agcaagcgtt
     6541 cgcccgcgcg cgcgcgccgc acggccaggt cacagctcgg cagcagatcg gcgtcgtacg
     6601 gatgtgacgg ctcgcccagc tcgagcatca cgtagttcga ggcatcgacg acgttgttga
     6661 tcggcgtcat tcccgcgagc agtaagcgac gcttgagcca gcggggggat tcggtgacct
     6721 gcacgttctc gatcaaagtc agccgcagca cccggcccac gtcgggcgct tggatttgcg
     6781 gcgcgagctt ggatggtcct tcgtgcgaga tccggatcgc cgcgattgga gccggtggcg
     6841 tgagcggttg cgcgagcaga gccgcgacct ctcgcgccac ccccaacatc gactggcagc
     6901 gcgcgatgtt cggcaaaatg gcgagcagca ggacccagtc cgcttcgctc ttcgtggcgg
     6961 agaacacctg actaacgggc gcgccgattg gcgcatccgc gccgaacagg aaggcatgct
     7021 cggcgtccga gccgattccc aacgtggccg cgctcgccag catcccggcc gagcgcacgc
     7081 ccctcacctc ggactcggac acgtcctcga gctgctcgag gtttgacgag agcaacgtgg
     7141 ctccgggcag cgcgaccgcg agcttctgcc cgacgctgac accctcggcg cgcgtcacga
     7201 tgctgcgcgt gccgcccgta tctacgacca aacggaaacc cgcgcctccg ggcagcgcgg
     7261 agacctcgcg cacttcaccc accaccacgt tcggatcgag cgcgcccacc ccacgcacct
     7321 cttcgacttc gatgccggaa agagtcaggc gctcagccac ctcgagcgga tcgagggggc
     7381 tcgcgaagta ctgcatcagc caggagaacg ggactttcat gacgatcgat cagggtagta
     7441 ggcggccaag taggctaaaa cggaacgagg agggagccgc gattcgggag cggtgattgg
     7501 agaccggcga accgatcgtc acgcgcaacc cgagcggcca cgaacatcag cgaaactggc
     7561 gcaagaagcg caaatcgttc tgatagaaat gtcggatgtc gtcgatgcct tggagcaaca
     7621 tgaccatgcg ctctatgccc atgccgaacg cgatgccgcg tacggtattc gggtcatagc
     7681 caccgttctt cagaaccacc ggatgcacga gcccggcccc gagcagctcg agccagcccg
     7741 agttcttgca gacgcgacaa cccttgccgc cgcatagcgt gcagcgaata tccacttcca
     7801 cgctgggctc ggtgaacggg aagtagctcc cgcgcagacg aatctgctga tcacggccga
     7861 acatgctgcg cgcgaagcgc aagagcacgc ccttcaaatc gctgagccgc acggtcttgc
     7921 ccacgagcag cccttcgatt tggtggaact gaatctccga gcgagtggtg atgttttcgt
     7981 ggcggtaaca caggccgggt aggatcacgc gcaccggctc gggcgcgtac agtcgcatcg
     8041 cgcgcacctg cccagccgag gtgtgggtcc tgagcaccgt gcggtcggac agatagaagg
     8101 tgtcctgcat gtcgcgtgca gggtggtccg cgggcatgtt caggagctcg aagttgaact
     8161 cgtcgagctc cgcgtgcggg gactcgaagg tggtgaagcc catctcgtgg aacaccttct
     8221 ccacctgacg aatcgcggtc gtgatcgggt gataaccgcc cgtcacgacg ctgcgcgagg
     8281 gcaacgtcac gtcgatgctc tcgcgctgaa tggcgcgcga aagctcgagc tccgaaatgc
     8341 gctgtttctg gccgcgaaag gcggcgtcca aagccgacgc cgcggcgttc accttggcac
     8401 cgaaggcttt gcgttgctcc accggctctg cgccgatgcg cttgcgccac tggttcatcg
     8461 taccggaggg agccaaatgc tttcggtacc aaccttcgag cgccttctcg tcactcagct
     8521 cgg
//
LOCUS       DASTKU010000343         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1181281, whole genome shotgun sequence.
ACCESSION   DASTKU010000343 DASTKU010000000
VERSION     DASTKU010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181281"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..1531
                     /locus_tag="VFQ61_12040"
     CDS             398..1531
                     /locus_tag="VFQ61_12040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9955231.1"
                     /translation="MTSSGGRSGSGGTSTTAGTSTSGGAGSTSGGATNGSGGGSAGST
                     SAGGTGAPEVQLIGRMDKSDSAGPRFAWSGSGVVARFEGTSVAASLKGGQQYTVLIDG
                     ELKPKLVAKDGTNSLASGLTSGAHTVELYRRTEANQGEAQFLGFDFGGGRLLPPPPRA
                     TRRLEIVGDSITCGYGNEGSDMNCPFSPDTENHYATYGAIAARNLNAELVTVAWSGKG
                     VVCNYGDDTNSCSDPMPTYYERTLPDRADSRFDFSTYQADAVIINLGTNDFSTTMDPT
                     AAQFEKGYEDLLKLIRSKHTKATILCTVAPLLSGDDLMKAQTSIANVVKKLNDAGDSK
                     VKTFTLGAVASAEGFGCDWHPSIASHARMGAELTTVLKSTLNW"
     gene            complement(1609..>2498)
                     /locus_tag="VFQ61_12045"
     CDS             complement(1609..>2498)
                     /locus_tag="VFQ61_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HET9955232.1"
                     /translation="NNDDAKQPGSKGLAAGQQEVDDKGRLAFKVEAAAYLFERTGEES
                     YRDFFDKNYATVTPNYASYWEIERHEAALDYAMQKGATASVVSDIKSKFLAQIKGEGA
                     VLQSATTHQEAYRAPLSDYTWGSNQVKAGAGRLLLLIEDYELEPATAPAARAAAADYL
                     HYLHGVNPLGLVFLTNMDKAGAENSAKTLYHTWFAYRSAKWQEVGATTPGPAPGFLVG
                     GPNPSYTLDGCCTDGSKCYGSADFSFCSLALAPPLDQPPAKSYLQFNHGWPANSWAVT
                     ENSNGYQVQYIRLLSSFID"
BASE COUNT          478 a          709 c          848 g          463 t
ORIGIN      
        1 gatgagaaac agttgcgccg cgaacgggga acagagtccc acattcgccc caatgaaaca
       61 cgactctggc caatccaccc cgcgcacggg ttgcgcgcga agctgatcgc gacttcgaaa
      121 aagagctgat cgacaccaag cgatggagta tttgcgctgg cctcgagcat cgggcctggt
      181 catgctctgc gcgatggatg cattagcgag gtattggcgg tcgtctgcgc gcggggcggc
      241 cctggtttcg atctggtgtg gcgtcgcgag cagtgggtgc tcggctggcg cgggacccat
      301 cgaagttgcg ggtggcagtt cctttggcgg tttctcaggc gcatcgggag caggcgggag
      361 cggcggctcg agcggaagcg caacgagcaa cgcgggcatg acctcgtccg gagggcgcag
      421 cgggagcggt ggaacgagca cgaccgcggg taccagcacg agcggaggcg cgggcagcac
      481 gtcaggcggt gcgaccaacg gcagcggcgg cgggtcagcc ggcagtacat cagccggcgg
      541 cactggggca ccggaagtgc aactgatcgg tcgcatggat aagagcgaca gcgcggggcc
      601 gcgttttgcg tggtccggct cgggcgtcgt ggcgcgcttc gaagggacgt cagtggcggc
      661 ttcgttgaag ggtggacagc aatataccgt gttgatcgac ggagagctaa aacccaagtt
      721 ggtcgcgaag gatggaacca actcgctggc ttcgggtttg acgagcggcg cccacaccgt
      781 ggagctttat cgcagaacgg aagccaacca gggtgaagct cagttcttgg gattcgattt
      841 cggaggaggg cgtctgttgc cacccccgcc gcgggccacc cgtcggctcg agattgtcgg
      901 ggactcgatc acctgcgggt acggaaacga aggctccgat atgaactgcc ccttctcccc
      961 agatacggag aatcactatg cgacttatgg ggcgatcgcg gctcgcaact tgaatgcaga
     1021 gctcgtcacg gtagcctggt ccggcaaggg cgtagtctgc aattacgggg atgatacgaa
     1081 cagctgcagc gatccgatgc ccacctatta cgagcgcacg ttgcccgatc gcgccgacag
     1141 tcgcttcgat ttctcgacct atcaagccga cgccgtgatc atcaatctgg gcacgaacga
     1201 cttcagcacg acgatggatc cgacggcggc gcagttcgag aagggctatg aggatctgct
     1261 gaagctgatc cgaagcaagc acacgaaggc cacgatcctg tgcacggtcg cgccgctgct
     1321 cagcggtgac gatttgatga aggctcagac ctcgattgca aatgtggtca agaagctgaa
     1381 cgacgccggc gacagcaagg tgaagacctt cacgcttggc gcggtggctt ctgcggaggg
     1441 atttggctgc gattggcacc ctagcatcgc gtcgcacgca cgcatgggcg cggagctcac
     1501 caccgtactc aagtccaccc tgaattggtg agcttcttgg agccgaaacg gcgcgaggcc
     1561 agaacccgtg agttccggcc tcgctatttc ttcggccaat gaacgcgctc agtcgatgaa
     1621 cgacgagagc aatcgaatgt actgcacctg atagccgttg ctgttctcgg tgacggccca
     1681 ggagttcgcg ggccagccgt gattgaattg caggtacgac ttggccggcg gttggtcgag
     1741 cggcggcgcg agggcgagcg agcagaagct gaagtccgcc gaaccgtaac acttgctgcc
     1801 gtccgtgcag cagccatcga gtgtgtagga tgggttaggg ccaccaacga ggaagcccgg
     1861 ggccgggccc ggagtggtcg cgccgacctc ttgccacttg gcactgcggt acgcgaacca
     1921 cgtgtgatag agcgtcttcg cggagttctc cgcgccagcc ttgtccatgt tggtgaggaa
     1981 cacgagaccg agcgggttca cgccgtgcag gtaatgcagg tagtccgcgg ctgcggcgcg
     2041 cgcagccggg gccgtggcgg gctcgagctc gtagtcctcg atgagcagga gcaatcgacc
     2101 ggcgccggct ttgacttggt tgctacccca agtgtagtcg gagagcggag ctcgataagc
     2161 ttcttgatgg gtcgtggccg attggagaac ggcgccttca cccttgattt gggccaagaa
     2221 cttgctcttg atgtcgctca ccacgctagc ggtagcgcct ttttgcatgg cgtagtcgag
     2281 ggccgcctcg tggcgctcga tttcccagta agaagcgtaa ttgggggtta cggtggcgta
     2341 gttcttatcg aagaagtcac ggtaactctc ctcgcccgtg cgctcgaaca agtaagcggc
     2401 ggcctcgacc ttgaaggcca agcgcccctt gtcgtcgacc tcttgttgac ccgcggctag
     2461 gcccttcgat cccggctgct tggcgtcgtc gttattga
//
LOCUS       DASTKU010000344         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_512677, whole genome shotgun sequence.
ACCESSION   DASTKU010000344 DASTKU010000000
VERSION     DASTKU010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512677"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2088)
                     /locus_tag="VFQ61_12050"
     CDS             complement(<1..2088)
                     /locus_tag="VFQ61_12050"
                     /inference="COORDINATES: protein motif:HMM:NF020926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-binding domain-containing protein"
                     /protein_id="HET9955233.1"
                     /translation="MKQALQGVKEQIKEYTEQIKTLEARIRELEENPSSASTVLDDLG
                     KPIADAEAELVELRDTERAYRRLRAELQNKYPLNVLADAGVIPNYAFPEPGVTLSALL
                     RGPAQSGEPADPTEAAGGPPAGKQAKRGTESDRKGGKRVEYLRSASQALREFAPFNTF
                     YAEGHKIRVSQLDLGSRASAIETWRLCKACHHMEPELPNTPAANECPRCENTSWPDVG
                     QRRALVRFRRAVSIMNRLDASTAEDSEERDRESYRLVELIDVGPENWSGTRLVRRSDL
                     VFGVELLQRQLLREVNFGRRGDIAKSAQLVGQDIPQRGFLTCRLCGRVSDSEARAEHT
                     PLCPVRRKGEKPAFERLYLYREFHSEAIRLLLPVSQLEEPALLHSLKAALFLGFRKKF
                     LGHPDHLTITLANEPRGSELRRFLVVYDTVPGGTGYLADLWKQDGMLDVLERARDAMQ
                     SCRCTEDESKDGCYRCVYAYQQSHEIPHISRSRAIELIDAILAARADLQDAHTLSEAP
                     IDDLTESELERKFLRALEERASVPGHGWTSTQQRGKACHVLTTPNSQWLIEPQVDLGE
                     ESGVHRASRPDFLIRCLRAHEELPIAVFCDGLRYHVCPDQEISLLGEDIGKRRAILES
                     GRFRIWSVTWKDLESSSGVTTLLTRVQRQLYNQFFAKDPEAHWLREDDLRSRSSFDLL
                     WEYLEHPNAEVWAG"
BASE COUNT          374 a          675 c          682 g          419 t
ORIGIN      
        1 gccggcccac acctccgcgt tgggatgctc caagtactcc cagaggaggt cgaaactgga
       61 acggctacgc aggtcatcct cccggagcca gtgcgcctcg ggatcctttg cgaagaattg
      121 attgtagagt tggcgctgca cccgcgtgag cagcgtggtg accccggagc tactctcgag
      181 atctttccaa gtcaccgacc agatccggaa tcggccagac tcgaggatgg cccggcgctt
      241 gccgatatct tcccccagta aactgatttc ttggtcagga cacacgtggt agcgcagacc
      301 atcgcagaac acggcgatgg gcagctcctc atgggcgcgc agacaccgta tcaagaagtc
      361 cggccgggac gcgcgatgaa ctcctgattc ttccccgaga tccacctgtg gctcaatcaa
      421 ccattggcta ttgggggtgg tgagcacgtg acaggctttg ccgcgttgtt gggtcgaggt
      481 ccagccatgg ccgggcaccg aggcgcgctc ctccagggca cgcaagaact tgcgttcgag
      541 ctcgctctcg gtcaggtcat cgatcggtgc ttccgagagc gtgtgggcat cctgcaagtc
      601 ggctcgcgcc gccaggatcg cgtcgatcag ctcgatggcc cgtgaccgag agatgtgcgg
      661 gatctcgtgc gattgttgat aggcgtagac gcagcggtaa caaccgtctt tgctctcgtc
      721 ttcggtgcag cgacagctct gcatggcgtc tcgggctcgc tccagcacat ccagcatccc
      781 gtcttgtttc cagagatcgg cgaggtaccc ggtcccgccg ggcacggtgt cgtaaaccac
      841 caagaagcga cgaagctcgc tgccgcgcgg ctcgttcgcg agcgtgatcg tcaagtgatc
      901 ggggtggccg agaaacttct tgcgaaatcc caggaacagg gctgctttta ggctgtgcag
      961 caaggccggc tcttcgagct gggacacagg caagagcaaa cggatggcct cggagtggaa
     1021 ctctcgatac aggtacagcc gttcaaaggc aggcttctcc cctttgcggc gcaccgggca
     1081 gagcggagtg tgctccgccc gggcttcgga gtccgagacg cgcccgcaga gcctgcaggt
     1141 gagaaagccg cgctgcggaa tgtcttgtcc gacgagctgg gctgacttcg cgatgtcgcc
     1201 gcgccggcca aaattgacct cgcgtagcag ctgtcgctgc aaaagctcta cgccaaagac
     1261 caaatcggag cgccgcacga gccgagtacc ggaccagttc tcgggcccca cgtcgatcaa
     1321 ctcgacgagg cggtagctct cgcgatcgcg ctcctcggag tcctctgcgg tgctggcgtc
     1381 gaggcggttc atgatcgaga ccgcccgtcg gaagcgcacg agcgcgcggc gttgccccac
     1441 atcgggccag ctcgtgttct cgcagcgcgg gcattcgttc gccgccggcg tattcggtag
     1501 ctcgggttcc atgtggtggc aggctttgca gagccgccac gtctcgatgg ctgaagctcg
     1561 gctcccgaga tccagctgtg agacacggat cttatggcct tcggcgtaaa aggtgttgaa
     1621 cggcgcaaac tcgcgaaggg cttggctggc cgagcggagg tactcgacgc gcttgccgcc
     1681 tttccgatcg gactctgtac cccgtttcgc ctgcttgccc gcaggagggc cgcccgccgc
     1741 ctcagtggga tctgccggct ctcccgactg ggccggtccg cgcaacagcg cactcagggt
     1801 cactccgggc tctgggaatg cataattggg aatcacgccg gcgtcggcca gcacgttgag
     1861 cgggtacttg ttctgaagct cagcgcgcaa gcgccgatac gcgcgctcgg tatctcgtag
     1921 ctcgaccagt tcggcttcgg catcggcgat cggtttgccg agatcgtcca acacggtgga
     1981 tgccgagctg gggttctcct ccagctcgcg aatccgtgcc tcgagcgtct tgatctgttc
     2041 ggtgtactct ttgatttgtt ccttgacgcc ttgcagggcc tgcttcatgg gcgccgccaa
     2101 accttcaccc gaacccaaat ggcgcaggcg ctggacgttc gcctcgctca
//
LOCUS       DASTKU010000345         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_958180, whole genome shotgun sequence.
ACCESSION   DASTKU010000345 DASTKU010000000
VERSION     DASTKU010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958180"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1904)
                     /locus_tag="VFQ61_12055"
     CDS             complement(<1..1904)
                     /locus_tag="VFQ61_12055"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9955234.1"
                     /translation="MSQALEKPLALYTPFQFPAQAAIFSVAVDGTDQPAKYISHASWG
                     ESQQQPGVGTAWLLLVDADARMGSGFDDAKQVAQQFIQSLGPNDIVNVMFFNDRQIVK
                     DSRWMPASQRARAVQFVQSVAGTFPSSARNRTLLTLIKNGATDAFKSLGNQGEGVQVP
                     LHQAMVLLSSGFAGTDPSSTGPAGLQLNQYLSNGRFPEDNTALPKAPVPVVSIYFPHQ
                     TYQEFALNSLEFMQNLVNTELGGFFTVVQKGGGTRAPQIVNAVRQRFAKMQIVKWKVS
                     CVAPSITQSFKLVFNNTGTPVLGDNSFKDVPIGIDPSTWPLDVNVEYTVDSAKRQNGV
                     YPGGKFKVFGDFCWGGDKSRAEVYFLPAGQQLPTALAGTDLDKAKRTQQQLIAMGMKG
                     TADEASDTYVTFVAPDNEKILHGSGEQAVVRLVLYDNRAHRTSGVTADTVLKLKGIPA
                     PLPIMSILIGLLGVVVIALLVVIVLRSGGRKRGGAAPLAPIVAGGPPAYAPPPGYGAP
                     PPGPSPYAAAPPAYAPPQQPVPPMQPPQPAYGGGMPSSAVLQGAAGTFTILNGQELKA
                     GRDPNQCGIVLHEPRVSGVHATLKLEGGQLLIRDENSNNGTLVNNVRLSPSVWSPVPS
                     GSLLRLGPVEL"
BASE COUNT          342 a          629 c          667 g          379 t
ORIGIN      
        1 agctcgacgg gaccgagccg cagcagtgaa cccgagggca cgggggacca aaccgacggc
       61 gagagccgca cgttgttcac cagggtaccg ttgttgctgt tctcgtcccg aatcaaaagc
      121 tggccacctt ccaacttgag cgttgcgtgc accccagaga cccgaggttc gtgcagcacg
      181 ataccgcact gattgggatc gcggccagcc ttcagctctt gtccgttcag gatcgtgaac
      241 gtgccggccg cgccctgcag gaccgcggaa gagggcatgc cccctccgta ggccggctgc
      301 ggaggttgca tgggtggcac aggttgctgc ggcggtgcgt aagcgggcgg tgcagcggcg
      361 tagggcgacg gtccaggcgg tggcgcaccg taacctggag gcggcgcata agcgggcggc
      421 cctccggcca cgattggggc gagcggcgcg gcaccacctc gtttgcggcc accgcttcgc
      481 aggacgatca cgacgagcaa cgcaatgact acgaccccga gcaagccgat cagaatggac
      541 atgatgggca acggcgcggg gatgcccttg agcttcagta cggtgtcggc cgtaacaccg
      601 ctcgtgcggt gagcgcggtt gtcgtaaagc accagccgca ccacggcttg ttcgccggag
      661 ccatgcagga tcttctcgtt gtccggcgca acgaacgtca cgtaagtgtc gctcgcctcg
      721 tcggcggtgc ccttcatgcc catcgcgatc agctgctgct gcgtgcgctt ggccttgtcg
      781 agatcggtcc ccgccagcgc cgtcggaagc tgctgccccg cgggcaagaa gtaaacctcg
      841 gctcggctct tgtccccgcc ccaacagaag tcaccgaaga ctttgaactt gccgccggga
      901 taaacgccgt tttggcgttt cgcgctgtcg accgtgtatt cgacgttgac gtcgagcggc
      961 caagtcgacg gatcgatccc gatcggcacg tctttgaagc tgttgtcgcc cagcacgggg
     1021 gtgccggtgt tgttgaagac gagcttgaaa gactgcgtga tggagggcgc cacacaagac
     1081 accttccatt tcacgatctg catcttcgcg aagcgctgcc gaactgcgtt cacgatttgc
     1141 ggcgcacggg tgccgccgcc cttctgcacc acggtgaaga agccgcccag ctcggtgttc
     1201 accaggtttt gcatgaactc gaggctgttc agcgcaaact cttggtacgt ctggtgcggg
     1261 aagtagatgc tgacgaccgg taccggggcc ttcggcagcg cggtgttgtc ctcgggaaag
     1321 cgcccgttcg aaaggtattg attgagctgc agcccggcgg ggcccgtgct cgaaggatcg
     1381 gtgccggcga agccgctcga gagcagcacc atggcttggt ggagcggcac ctgcacgcct
     1441 tcgccctgat ttccgagcga tttgaaggcg tcggtcgcgc cgttcttgat cagcgtgagc
     1501 agcgtgcggt tgcgcgcgct cgacgggaac gtacccgcca cgctctgcac gaactgcacc
     1561 gcgcgggcgc gctggctcgc cggcatccag cgggagtcct tcacgatctg ccgatcgttg
     1621 aagaacatca cgttgacgat gtcgttcggg ccgaggctct ggatgaattg ttgcgcgact
     1681 tgcttggcgt catcgaagcc gctgcccatg cgagcgtccg cgtcgaccag gaggagccag
     1741 gccgttccga cgccgggttg ctgctggctc tcaccccaag aggcatgcga gatgtacttg
     1801 gcgggctgat ccgtgccgtc caccgccacg ctgaagatcg cggcctgggc cgggaattga
     1861 aagggcgtgt agagcgcgag cggcttctcc agcgcctggc tcatgcaatc caattgcgcg
     1921 ttgcccgtga gccctgcgca agggccaatc gcgtcgctca cgcgtttgct ctgcgagagc
     1981 tcgatcaccg tcgtcagtac gggatttccg ttttctt
//
LOCUS       DASTKU010000346         4551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_66494,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000346 DASTKU010000000
VERSION     DASTKU010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4551
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66494"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="VFQ61_12060"
     CDS             <1..1481
                     /locus_tag="VFQ61_12060"
                     /inference="COORDINATES: protein motif:HMM:NF036933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IgGFc-binding protein"
                     /protein_id="HET9955235.1"
                     /translation="VVVSNPQPDVSTHVSIEQDDSAPGEEPQIRSVARATIPPFSLRV
                     FKLGPREVDGSAPGTFDTGTGTALTRAAFRIQSDMPVVAYQFNPLENVSVFSNDASLL
                     KPVEALIPSTAELSDSYVVLGWPQTIARSSDPATNFDEDLRAFLTLVGTQRETHVRIH
                     TTARVIAGGPVPETEAGGVIEAVLGPFEVLNLETGDFGADFTGTRIAADGPVVVFSGS
                     EASDAPRFDELSERSCCADHLEEQLDPIRTAGQSFIATVSPNRMKALVGAGAEFAEVL
                     PQPEYFRLIAATALGARVQTTLAGHEQLELAEKGSFVDLTSTRDFAIWSDAPVMMMSV
                     SPSQAAASIPTILPGGDPSSLIVPPTEQFRSTYVFLTPDKYSFDFVRIIAEPATKIVF
                     DGEPLETLEGCSSSALELSGGSAAAANELAGEWIVHTCQLSFPIIDSTQTAPQNLRAG
                     RQNDGVHRLAASRPVGVLVDGFDAYVSYAYAGGTELEYIVPE"
     gene            complement(1504..3507)
                     /locus_tag="VFQ61_12065"
     CDS             complement(1504..3507)
                     /locus_tag="VFQ61_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955236.1"
                     /translation="MLNVADELSRVRRVSVSDWRHSRRARHLLAACSFLAACAARLTA
                     ARADWLVPDQARSSDARAAQPASRDVRTSKPGTTTILYADLDDDDDDGLADAQQPQLR
                     ASQHGDLLWLRAPQSNARFPILTDRVRVVVGSQPLPLGARVPATTRLGLQGVAAGHVP
                     SEAVGGFGDLSVIEFLAMDMHGSRVDLARSHASLSRVLPDFLATEPSPAAEAMTRVGL
                     DEDALRWVAIGESTQLPEQVDIVSLAADGHGLDELRRVELWAMPCPSHVAPELACRGT
                     ALIRAVVDRIDRNHPESARRSLRAEVGGRVQVRVGERKVSSIRVGGPRSTAVGPIGRY
                     RAKLAVHLFREALDGPPALPGSDAQALERARAEVQAASLLWGQCGIHFGLERDLMVEL
                     LNPPVPYLLSLGSLLGIPASGGELHFRVGARRVHVSTRAGQTPLEVATRVVAALSRLG
                     LRPELSRNPRVEPGALETADVLVFESADTSSQLRRHPVRIEPDGQLALNTDPSLSVQI
                     GSVDFADGLSHFDDYDSAAGTLEERTLLKANLDDDPASIDVFIVPTFSGYGRVGESFL
                     DGLRSTVQNAVIIDRSAVLSGIQSHVLAHEIGHILLNMPGHPDDYGVDQPSALMDADA
                     TDPSIFGPRRLSIAECERVLRERGPSAPAPLLQEWPLFAPPRR"
     gene            3757..4179
                     /locus_tag="VFQ61_12070"
     CDS             3757..4179
                     /locus_tag="VFQ61_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955237.1"
                     /translation="MNRFLLLVAFGTWVLACKSEDQAPPQQGGYPPQYPQQQYPQQQY
                     PQQQYPQQQYPQQQYPQQQYPTAAQPAPVVVPTAAAPATTAPAGTMSTPSPLAFPCSS
                     DAQCFTHRCNTQVGRCAWPCQSDADCVTGRRCQASQCL"
     gene            4235..>4551
                     /gene="cyaY"
                     /locus_tag="VFQ61_12075"
     CDS             4235..>4551
                     /gene="cyaY"
                     /locus_tag="VFQ61_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HET9955238.1"
                     /translation="MDEQTYESLAYPELKALVSALDALELEGLEAELASDILTMEFED
                     GTRYVVNSHRAARQIWMAAERSAWHFDWQPAERHWVATKTGDELWSTLARLIGSKLGR
                     EIA"
BASE COUNT          842 a         1489 c         1436 g          784 t
ORIGIN      
        1 cggtggtggt cagcaatcct cagcccgatg tctcgaccca cgtcagcatc gagcaggacg
       61 actctgcgcc cggcgaagag ccacagatac ggagcgtggc ccgagccacg attcccccgt
      121 tttctttgcg cgtattcaag ctcggcccgc gcgaggtgga tggctccgct ccgggcacct
      181 tcgacacggg caccggcacg gcgctgaccc gcgcggcctt ccggattcag agtgatatgc
      241 cggtagtggc ctatcagttc aatccgctgg agaacgtcag cgtcttctcc aatgacgcct
      301 ctctgctcaa accggtcgag gcgctgatcc catccaccgc cgagctgagc gattcgtacg
      361 tcgtgttggg ctggcctcag acgatcgccc gctccagtga tccggccacc aatttcgatg
      421 aggatttgcg agcattcctg acattggtgg gcacccaacg cgagacgcac gtccggatcc
      481 acaccaccgc gcgcgtgatc gcgggaggcc ccgtgcccga gacggaagcg ggtggagtga
      541 tcgaggcggt gctaggcccc ttcgaggtgc tgaacctaga gaccggagat ttcggcgcgg
      601 atttcacggg gacacgcatc gcggcggacg gcccggtcgt cgtatttagc ggcagcgagg
      661 ccagtgacgc ccccaggttc gacgagttgt ccgagcggag ctgttgcgcg gaccacctcg
      721 aggaacaact ggatccgatc cgcacggcgg gccagtcgtt cattgccacc gtttccccca
      781 atcggatgaa ggcgctagta ggcgcggggg cagagtttgc cgaagtgctg ccgcaaccgg
      841 aatactttcg gttgatcgcg gcgaccgcgc tgggcgctcg cgtgcaaacc accttggcgg
      901 gccacgagca gctcgaactc gcggagaaag gcagcttcgt ggatcttacg agcacccgcg
      961 actttgccat ttggagcgac gcgccggtta tgatgatgag cgtgtccccg agtcaggccg
     1021 ccgcaagcat cccgacgatc ctgcccggag gagacccgag ctcgctgatc gtgccaccca
     1081 cggagcagtt ccggtccacg tacgtgtttc tcacacccga caagtacagc ttcgacttcg
     1141 tacgaatcat cgcggaaccc gcgaccaaga tcgtcttcga cggcgaaccg ttggagactc
     1201 tcgagggttg ttccagcagc gcactcgagc tcagcggggg cagcgcggcc gccgccaatg
     1261 agttggccgg cgaatggatc gttcacactt gccaactcag cttcccgatc atcgactcga
     1321 cccaaacggc tcctcagaat ctgagggccg gcagacaaaa cgacggcgtg caccgtctcg
     1381 cggcgagccg tcccgtcggt gttctcgtgg atggcttcga tgcctatgtc agctacgcgt
     1441 acgccggtgg caccgagctc gagtacatcg ttccggagtg aattgaatga gttctcgctc
     1501 ggctcagcgc cgcgggggcg cgaatagagg ccattcctgc agcagcggcg cgggggcgct
     1561 cggcccacgt tcgcgcaaga cccgctcgca ttcggcgatg gaaagacgac gcgggccaaa
     1621 gatgctcgga tcggtcgcgt cggcatccat caacgcgctg ggctggtcca ccccgtaatc
     1681 gtcggggtga cccggcatgt tgagcaagat gtggccaatt tcgtgggcta gaacatgact
     1741 ctggatgccg gacagtaccg cgctgcgatc gatgatgacc gcgttttgca ccgtgctgcg
     1801 tagcccgtcc aagaaagact ctccgactcg cccatagccg gaaaacgtcg gcacgatgaa
     1861 gacatcgatg ctggccggat cgtcgtccaa gttggctttc agcaacgtcc gctcctcgag
     1921 cgttcccgcc gccgaatcgt agtcgtcgaa gtgcgataat ccatccgcga aatccacgct
     1981 gccgatctga acactcaggc tcggatcggt gttgagagcc aactgcccat cgggttcgat
     2041 ccgaacgggg tgccttcgaa gctggcttga cgtgtccgcg ctttcgaaaa ccagcacgtc
     2101 cgcggtctcg agcgcccccg gctcgactct tgggtttcgg ctgagctccg ggcgcagccc
     2161 caagcgcgac aacgcggcga ccacgcgggt cgccacttcg agcggcgtct gacccgcgcg
     2221 cgtcgagacg tgcacacgac gcgcgccgac gcggaagtgc aactcacctc cactcgcggg
     2281 gatccccagc agcgatccga gcgacagcag atagggcacg ggcgggttca gaagttccac
     2341 catcaaatcg cgctccagcc cgaagtgaat tccgcactgc ccccagagca ggctcgccgc
     2401 ctgcacctcg gcgcgggcac gctccagtgc ttgcgcgtcc gagccgggca aggcgggcgg
     2461 gccgtcgagt gcttctctga aaagatggac cgccaacttc gctcgatagc gtccgatcgg
     2521 cccgacggcg gtggagcgcg gccccccgac gcgaatgctg gagaccttac gctcgcctac
     2581 ccgcacctgg actcggccgc ccacctcggc ccggagcgac cgtctggctg attctggatg
     2641 attacgatcg atgcggtcga cgacggcacg gatcaacgcc gtcccgcggc aagctagctc
     2701 cggagcgacg tggctcgggc aaggcatggc ccaaagctcg acgcgacgta gctcatccaa
     2761 gccgtgaccg tccgccgcca gcgaaacgat atctacttgt tctggcaact gagtggattc
     2821 accaatcgcc acccagcgca aagcgtcttc atcgagcccg actcgcgtca tggcctccgc
     2881 ggctgggctg ggctcggtgg cgagaaagtc gggcaatact cggctcaatg acgcgtgcga
     2941 gcgggccaaa tccacgcgcg agccgtgcat atccatggcc aggaactcga tcaccgagag
     3001 atccccaaaa ccgcccacgg cttcgctggg aacgtgccct gctgcaacgc cctgcaatcc
     3061 gagccgcgtc gtggccggga cgcgtgcgcc aagcggcaga ggctgcgatc ccacgactac
     3121 ccgcacccga tcggtgagga ttggaaaacg agcgttggac tgtggtgccc gcagccagag
     3181 caggtccccg tgctggctcg ctcgcagttg gggctgctgt gcgtccgcca ggccgtcgtc
     3241 gtcatcatcg tcgaggtcgg catacaagat ggtcgtggtg cccggcttac tggttcggac
     3301 gtcgcggctt gctggctggg cagcgcgcgc gtcgctgctt cgcgcttggt cggggacgag
     3361 ccaatccgcc cgtgccgctg taagacgtgc ggcgcacgcg gcgaggaatg aacatgctgc
     3421 gagcagatgc cgcgctcgcc gcgagtgcct ccagtcggac accgaaactc gacggacgcg
     3481 gctcagttcg tcggcaacgt tcaacacgac ctccgtcgct ctcccgcatc gtacaatggc
     3541 tctgcgcgaa gcgcccgccc gggcagggct cagcgggcgg ttgcacggcg ggaatgggaa
     3601 catttcggct tttcggtcga ggcctccagc cgccccgggc tccactcgag cgggcgagcg
     3661 gattcgctta agactcgcat ttggagagca gttggtgtgg actgtcggcg tatgaagaag
     3721 cgtgcctcag aggccactct tcacggagga cgacagatga accgcttcct gcttcttgtt
     3781 gcattcggaa cctgggtgct cgcttgcaaa agtgaggatc aagccccgcc tcaacagggt
     3841 ggctatccgc cccagtatcc tcagcagcag tacccgcagc agcaatatcc tcagcaacag
     3901 tacccgcagc agcaatatcc tcagcaacaa tacccgcagc agcaataccc gacggccgct
     3961 caacctgcgc cggtcgtggt tccgaccgcg gccgcgcccg ccacgacggc gccggcgggt
     4021 accatgtcca ccccatcgcc actggcattc ccatgctcga gcgacgcgca gtgtttcacg
     4081 catcgctgca acacccaggt gggtcgctgc gcatggccgt gccagtctga cgcggattgc
     4141 gtgaccggaa ggcgctgcca agcttcacag tgcttgtgag gcttcgcttc gtggtgccgg
     4201 tgaggctttc gcggttgtaa cggaagagaa gactttggac gaacagacgt acgaaagttt
     4261 ggcttacccc gaactcaaag cgttggtctc cgcactcgac gcgttggagc tcgaggggtt
     4321 ggaagcggag cttgccagcg acattctgac gatggagttc gaagacggaa cccgatacgt
     4381 cgtcaactcg caccgggccg cgcgccaaat ctggatggcg gcagagcgct cggcctggca
     4441 cttcgattgg caaccagccg agcgtcactg ggtcgcgacc aaaacgggag acgagttgtg
     4501 gtccaccctc gcccgcttga ttggcagcaa actcggacgc gagatcgcct g
//
LOCUS       DASTKU010000347         5765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_734752, whole genome shotgun sequence.
ACCESSION   DASTKU010000347 DASTKU010000000
VERSION     DASTKU010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734752"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(369..1904)
                     /locus_tag="VFQ61_12080"
     CDS             complement(369..1904)
                     /locus_tag="VFQ61_12080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9955239.1"
                     /translation="MTTESNPRYDEVPYQSHPFLRSHPRWLATIGAVFGMTAPAPDRA
                     RVLELGCASGGNLIPMAESLPDSQCVGVDLSSRQIQDGQRFRQASGLSNVELKQASIL
                     DIDESYGQFDYIICHGVYSWVPRNVQQRILEICGKLLTPQGLAYVSYNTLPGWGMLGV
                     LRDMMRFHASRFTEPSRQIAQSKALLNVLAKLVPSEDNPYGMLLKQSSQKLRDAADWY
                     IYHEHLEENNDPVYFHEFVTRAGMAGLQFLGEADLLSMLTNRLPPEVQAALKEVSTNI
                     VHSEQYVDFFRNRTFRETILCRRSVALNRVIDAEKIKPLSFATDLEAQASPVDVRNPG
                     EVTFSAPQRPSVGVSDPIFKAALVALRAAWPRYVPVLELQAQVRGRLELAPASEQNTR
                     EFIDRLWSWYTNHLVQMTSTPVHCANSASERPVASAVARGQLSLGRTLLTDLLHRPVR
                     LSAFEAAVLQRLDGSHDRAALSQALAGNRAVQELLAKEGRSLDASVQTALNRFAGLAL
                     LKS"
     gene            complement(2226..2888)
                     /locus_tag="VFQ61_12085"
     CDS             complement(2226..2888)
                     /locus_tag="VFQ61_12085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9955240.1"
                     /translation="MTQAVLLFGHADSDESSPSLNQLLAQRYTEGYIAAGGTVRRFDI
                     ATLQFDPVMRRGYRASQPLEPDLERVKEAIEQAQHVTWVFPTYWASPPAIVRGLFDRL
                     FLPRWAFSYEKDQPLPKGLLKGRSSRVVMTMDSPWFWYTFVNQRCIHRSFGGASLKFC
                     GFSPVQFTTVHGVRSLSPAARATWQERVYKLGIEDATRPSPRPASRLAPAPSARLDAP
                     RS"
     gene            2961..3869
                     /locus_tag="VFQ61_12090"
     CDS             2961..3869
                     /locus_tag="VFQ61_12090"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9955241.1"
                     /translation="MTHGRRERSLARWDDVHWFLVVLRKGTFTAAARALRTDQSTVSR
                     RIAALEAELGLALFERGRRNLVPTEAAQRLSETAQRIEAEIGHFADDVLGMHSEAVSG
                     RVRLALTEELAISFVIPKVLPKLRAAHPALAVDLVTSYRAADLMSHEADIALRFFQTP
                     RGDLVGRRIARLPTAILSSRRYAKRAAGLGLDELDWIQVELDGLAAVEKTWLESVTRR
                     APAMICSSYQVQLSAIRSGLGVGIGPAIQTALDKEFVVLDRPGVTPPVLDLYLVTRRS
                     IRGLPRIVAVMDALSDEIGGVNDERG"
     gene            complement(3901..>5765)
                     /gene="tgt"
                     /locus_tag="VFQ61_12095"
     CDS             complement(3901..>5765)
                     /gene="tgt"
                     /locus_tag="VFQ61_12095"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET9955242.1"
                     /translation="LEDLGARLLLANTYHLLQRPGLDALRRTGGLARWMGWSGGILTD
                     SGGFQIYSLAHACTIDDAGARFRPHPDGPVLTLTPELAIGAQEVIASDIMMVLDQCIP
                     TTADRETTRMALERTNAWAVRSLAARRDPERALFAIVQGGGFEDLRRQSAQALTAVEG
                     FDGFAIGGLAVGETRAEREDLTATVTELLPLDRPRYLMGVGTPIDLLEAVHRGVDMFD
                     CILPTALAQRGVAFTSRGRVELRRGVYGGDDGALDPVCRCEACRQVPRSYLHHLIKTG
                     EPTGWSLLAFHNLRFYTDLMLEMREAIRGGRFERLYGELKERLTAEDPSHAPGPAPRA
                     RRRSSRAIERGAFAIQLAPNGRGRIRHVGSGEVMHPVQDPNEEAQRLYVDQPRAIQRA
                     LAGAPITVWDVGLGAAHNAMALVSAISKTPGAGEVTLVSFECDTDALALALAHPRLFP
                     HLRHPAPHRLLARAAFAAPGLSWQLLLGDVRDQLGGAPAPDVIWWDPFSSSVDRELWT
                     LETFTRVAAYLSKPCELVTYSRSTAVRAALLGAGFYVARGVASGPKSETTHALFGVSD
                     PQSIGYALLDGSWLRQRQRSSAAYPADVEGSHRADFDARLTQHPQFAASARPAL"
BASE COUNT         1032 a         1815 c         1905 g         1013 t
ORIGIN      
        1 accttcagtc tcacagaaag gactttgcaa cgaatccctg ccgacctcga gcagacctcg
       61 tccgtcgtcg gcacgagatc cgggcgccgc cctcctcgtc ggtgtcgtgc gctcatgagc
      121 aagtctgtgt cggaggactc cacgtgagtg ggccatgatt ccgatcatct gttacgggcc
      181 gccctgcaac gtgcgcgact tcgcacgcgg tgcccccgag cgcggcccca ccgcgctcca
      241 tctctcgtta caccgcgccg gcccccgcct cgtacaagcg tctgctttgg aaagcacggc
      301 agcgcccgcg ttcagggatc aaaagctgcg agggcgaaag cgaagcaaca ggacgcccag
      361 caagccgctc agctcttgag tagcgcgagc cccgcgaacc ggttcagcgc cgtttgcacg
      421 ctggcatcca gagatcggcc ctctttcgcg agcagctcct ggacggcacg atttccggcc
      481 agagcttggc tcaaggcggc gcggtcgtgc gaaccgtcga gccgctgcag cacggcggct
      541 tcgaacgcgc tcagccgaac cggccggtgc agcaggtccg tgagcagggt tcgacccagg
      601 ctgagttgcc cgcgcgccac cgcgctcgcg acgggacgtt cgctggcgga gtttgcgcag
      661 tgcacgggcg tcgaggtcat ctgtaccagg tggttggtgt accaactcca gaggcgatcg
      721 atgaactcgc gggtgttctg ttcgctcgca ggggccagtt cgaggcggcc acgcacctgc
      781 gcctggagct caaggacggg cacgtagcgc ggccaagccg cgcgcaaggc aaccaacgcc
      841 gccttgaaaa tcggatcact cacgccaacg ctgggccgtt gaggcgcgct aaacgtgacc
      901 tcgcccgggt tacgcacgtc gacggggctg gcctgcgctt ccaagtcggt cgcgaacgac
      961 aagggtttga tcttctctgc gtcgatgacg cgattcagcg ccacggagcg gcggcaaaga
     1021 atcgtctctc ggaaggttcg gttgcgaaag aagtcaacgt actgctcgga gtggacgatg
     1081 ttggtcgaga cctcctttag cgcggcttgt acttcgggcg gcaagcggtt cgtcagcata
     1141 ctcagtaagt cggcctcacc caagaactga agccccgcca tgcccgcgcg cgtgacgaac
     1201 tcgtggaaat agacagggtc gttattttcc tcgagatgct cgtgataaat gtaccagtcc
     1261 gccgcgtccc gcagcttctg gctgctttgc ttgagcaaca tcccgtacgg gttgtcttcg
     1321 ctcggtacga gcttggcgag tacattgagc aaagctttgg attgcgcgat ttgccggctc
     1381 ggctccgtga agcggctggc gtgaaaccgc atcatgtcgc gcagcacccc cagcatgccc
     1441 cagcccggca acgtattgta gctgacgtac gcgaggccct gcggcgtgag cagcttgccg
     1501 cagatttcga ggatacgctg ctgcacgttg cgcggtaccc aagagtagac accgtggcaa
     1561 atgatgtaat cgaattgccc gtagctctcg tcgatatcta gaatcgatgc ctgtttgagc
     1621 tcgacgttgc tcagtccgct ggcttgccgg aagcgctgcc cgtcctggat ttgccggctc
     1681 gaaagatcga cgccaacgca ctggctgtcg ggtaacgact ccgccatcgg gatcaggttc
     1741 ccgccgctcg cgcagccgag ctccaagacc cgcgctcgat cgggtgcggg cgcagtcatt
     1801 ccgaacacgg cgccgatggt ggcgagccag cgcgggtgac tgcgcaggaa cgggtggctc
     1861 tggtagggaa cttcgtcgta gcgcggattg gactctgtgg tcatgaccct ctcgcgtccc
     1921 aacggccact gtgaggcgcg actacaccgg ggaagatgat gaaagcgcga gcgcagctcc
     1981 caaagctgcc caatccagct ccgtgcgtca ggcggatgac gatcgcgatc gctatcccgc
     2041 gcccgcctgg cgaggaccgc cagatcgcgc gagtcgttgc ggcgggggca ggcgcggtga
     2101 ttgggggtta cgtgattgga agtgactgcg ctcgcgccgc cgatcttcgt tcgctcgcgc
     2161 ccgtcacgag agcggccgaa atggagaatc acggagccag agtggtgatg ttcctgtcca
     2221 caccctcacg aacgaggtgc atcgagccga gcagaggggg cgggggctag ccgcgaagcc
     2281 ggtcggggcg aggggcgcgt cgcgtcttca atgccgagtt tgtacactcg ctcctgccac
     2341 gtagcgcgcg cggcggggga aagggagcgc acgccgtgaa cggtcgtgaa ctggaccggg
     2401 gagaatccac agaacttgag tgacgcgccg ccgaaacttc tgtggatgca gcgctggttg
     2461 acgaaggtgt accaaaacca ggggctatcc atggtcatca ccacgcgcga cgaacgtccc
     2521 ttcaagaggc ctttcggcaa gggctggtcc ttctcgtagc tgaaggccca acgcggcaga
     2581 aacaagcggt cgaacaggcc acgtacgatg gctggcggcg aggcccagta ggtggggaac
     2641 acccacgtga catgctgggc ttgctcgata gcctccttga cgcgctcgag gtcgggttcg
     2701 agcggctggg aggcgcgata tcctcggcgc atcaccgggt cgaactgcag ggtggcgata
     2761 tcgaagcggc gcaccgtgcc gcctgccgcg atgtaaccct cggtatagcg ctgggccagg
     2821 agttggttca aagacggcga ggattcatcc gaatctgcgt gtccgaagag gagtacggct
     2881 tgcgtcatgg cacccactac atgggataag tccgagcggt gggctaggct cacttctgca
     2941 tgcctgctat gcagcgctgc atgacccatg gcaggcgcga gcgctctttg gctcgttggg
     3001 atgacgtgca ctggttcctg gtggtactgc gaaagggaac ctttaccgcg gccgcgcgcg
     3061 cgctgcgcac ggatcagtcc acggtgagcc gtcgcatcgc cgcgctcgaa gccgagctcg
     3121 gcttggccct gttcgaacgg ggtcgccgca atttggttcc caccgaagcc gcgcaacgct
     3181 tgagtgagac cgcgcagcgc atcgaggcgg aaatcggcca cttcgccgat gatgtgctcg
     3241 gtatgcactc ggaggcagtc agtgggcggg tgcgactcgc cctgaccgag gagcttgcaa
     3301 tcagcttcgt gatcccgaag gtactaccca aactgcgtgc ggctcatccg gcgctcgcgg
     3361 tcgatctcgt cacgagctat cgcgcggcag atctcatgag tcacgaagca gacatcgcgc
     3421 tgcgtttctt ccaaaccccc cgcggggacc tcgtcgggcg gcggatagcg cgcctgccga
     3481 cggcaatcct cagctcgcgg cgttacgcca agcgtgcggc cgggctcggt ctagatgagc
     3541 tggattggat tcaggtcgag ctcgacgggc tggccgccgt ggaaaagacc tggctcgagt
     3601 cggtcacgcg gcgcgcgcca gccatgattt gttcgagcta ccaggttcag ctgtccgcga
     3661 tccgctcggg cttgggcgtg gggatcgggc ccgcgattca aaccgcgcta gacaaagaat
     3721 tcgtggtttt ggatcgcccc ggcgtgaccc cgccggtgct cgacttgtac ctggttactc
     3781 gacgatccat tcgcgggttg cctcgcatcg tcgccgtgat ggacgccctg tcggacgaga
     3841 ttgggggcgt gaatgacgaa cgggggtgag cccgactgcc gtgccgtcga ttcgcatcgt
     3901 tcaaagtgcg gggcgcgcag acgccgcaaa ctgcgggtgt tgggtgagac gcgcgtcgaa
     3961 gtccgcccgg tgcgagcctt cgacgtcggc gggataagcg gcgctcgagc gttgtcgctg
     4021 ccgcagccag ctgccgtcca gcagcgcgta cccgatgctt tggggatcgc tcacgccgaa
     4081 tagcgcgtgc gtggtttcgc tcttcggccc cgatgcaaca ccacgcgcga cgtagaaccc
     4141 ggcgccgagc agcgcggctc tcaccgccgt cgagcgggag taggtgacga gttcgcaggg
     4201 cttgctgagg tacgccgcta cgcgcgtgaa ggtctctagc gtccagagct cacggtcgac
     4261 actggaggag aacgggtccc accaaatgac gtcgggggca ggcgcgccgc caagctggtc
     4321 gcgaacgtca cccagaagaa gttgccaact caggccaggg gccgcaaagg cagcgcgcgc
     4381 cagcaatcga tggggggccg gatgacgaag gtgcggaaac aatcgaggat gcgcgagcgc
     4441 taacgcgagt gcgtcggtgt cgcattcgaa agatacgagc gtgacttcac ctgccccagg
     4501 ggttttggag atcgcggaga ccagcgccat ggcgttgtgg gcagccccaa gcccgacatc
     4561 ccacaccgtg atcggggccc ccgcgagagc gcgctggatg gcccggggtt ggtcgacgta
     4621 gagccgctgc gcctcctcgt tcggatcctg gacgggatgc atgacctcgc ccgagcccac
     4681 gtggcgtatg cgaccgcggc cattcggtgc caattggatc gcgaaagctc cgcgttcgat
     4741 ggcgcggctg gatctccttc ttgctctggg ggccgggccg ggagcgtggg agggatcctc
     4801 cgccgtcaag cgctccttga gttccccata cagcctctcg aagcgacccc cgcggatggc
     4861 ttcccgcatc tcgagcatca agtcggtgta gaaccgcaga ttgtggaagg ccaagaggct
     4921 ccaccccgtt ggctcaccgg tcttgatcag atgatgtaag tacgaccgag gcacctgacg
     4981 gcaggcttca cagcggcaca ccggatcgag ggctccatcg tcgccaccat acactccacg
     5041 tctgagctcg acccgcccac ggctggtgaa cgccactccg cgctgggcga gcgcagtcgg
     5101 gaggatacaa tcgaacatgt ctactcctcg gtgaacggcc tcgagcagat cgatgggagt
     5161 cccgactccc atcaggtagc gtggacgatc gagcggcagc aattccgtga ccgttgcggt
     5221 caggtcttcg cgctccgcgc gcgtctcgcc cacggccagt ccaccaatcg cgaacccgtc
     5281 gaagccttcg actgcggtca acgcctgggc actctgacgc cgtagatcct cgaacccacc
     5341 tccctgcacg atcgcgaaca gtgcgcgttc gggatcgcgg cgggctgcca acgagcgcac
     5401 ggcccaagcg ttcgttcttt cgagcgccat gcgcgtggtc tcacgatccg ccgtcgtggg
     5461 gatgcattga tcgagcacca tcatgatgtc actcgcgatc acctcctggg cgccaatcgc
     5521 cagctcggga gtcagggtga gcaccggacc gtccggatgc ggtcggaagc gggcccccgc
     5581 atcatcgatg gtgcaggcgt gggcaagcga atagatctga aaccctcctg aatcagtgag
     5641 tatgccgccg ctccacccca tccaccgcgc gagcccaccg gtgcgccgga gtgcgtcgag
     5701 cccggggcgt tgcagcaagt gataggtgtt tgccagcagc aggcgcgctc ccaagtcttc
     5761 gagtt
//
LOCUS       DASTKU010000348        10020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1851859, whole genome shotgun sequence.
ACCESSION   DASTKU010000348 DASTKU010000000
VERSION     DASTKU010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10020
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1851859"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..2022
                     /locus_tag="VFQ61_12100"
     CDS             370..2022
                     /locus_tag="VFQ61_12100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9955243.1"
                     /translation="MNATGLIETSSIRRAYRRLFELGRGGMARVYLAESLASGVRKLV
                     VLKILNPELCANPEMRASFRREAELSAQMNHPNVVQVLEVVEYAETPVIVMEYLDGAS
                     LSALLKYAGKHLSLRLRMHILAQVLAGLHHFHELQDLDGTPLNTVHRDVSPQNIMILH
                     DGPVKVLDFGIAKVNAPDNQVTRTGLIKGKIHYMPPEQLLGEAGLDRRADVFAVGVLL
                     WEAAAGRRMWEGKTEADVLRCMITGELPKVEDFAQDVPESVLQIVRRATHIDRTQRFS
                     SAQDMQVAIERGLAELGWAVQQRDLEEFMACHLGDRRRAHDLKVRDALRTPTSSDVMM
                     YCTPVTQGWAGPESGVDPSRITAAGSERAVSRVFRTSRRSWGWALGLLGALALLGAWL
                     ARREVRPAQGSLASPSTAPVHPESVELEVEASPPNAEIYLDGERLGVGRYSGSRRFAP
                     PKAVLEVSAPGYAKERKELSLVRDTALQIVLKPELSVQSTPVFAASADPVTGDKTESP
                     TRSAGPRTRRPGGSTSARGSRGCNPPYTFSADGVKTYKPECF"
     gene            2065..3156
                     /locus_tag="VFQ61_12105"
     CDS             2065..3156
                     /locus_tag="VFQ61_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET9955244.1"
                     /translation="MIDSRLARPLRGFRWLGFWFGCFACSLQMPSEDEVFAPKTAETS
                     DGGNTADMTGSIGGSSSMGGAPAGGKSAVGANGGRSVDVGGGSSTGGSGRGGSSQGGS
                     TNGGATKGGAHVGGVGGASEGGSSDGGSSGSAGEAGIGTGGGSSEGLVAHFEFEETTG
                     TVAANTVDQSRNASFVGTCSHVPGKRGNAVGIRNLENGTLDYVELPEDIFSGLKATTI
                     AMWVRDLSTARKGGRLFDFSRGAEETLYFAPDQPNPETSTNGGHLGGIHGGVSFVDIW
                     LTKSLTDKAWHHVAVTWSASSIDLYIDGSRAGGKSNPGVIASDLGATSTNWLGRTLDD
                     AFIALYAEMDDLRIYDRVLSRAEISSLYR"
     gene            complement(3260..3586)
                     /locus_tag="VFQ61_12110"
     CDS             complement(3260..3586)
                     /locus_tag="VFQ61_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET9955245.1"
                     /translation="MSDADDSDRTMPDDSESEQAIAVLPIEDSIDLHGFQPRDILDVV
                     VDYLEAAQLRGFREVRLIHGRGTGTQRARIRALLAELGRVESFRDAPAARGGWGATLV
                     YLKALE"
     gene            complement(3673..5757)
                     /locus_tag="VFQ61_12115"
     CDS             complement(3673..5757)
                     /locus_tag="VFQ61_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9955246.1"
                     /translation="MTELRNVQRDHWMRVVSVRTRLWLGSALFITLMITTGVFAIKRF
                     EALGAVLQIATREVPAKARASDALADAVHRGAAAKLALFTTDAPELTAQYAADVARAR
                     EGINSAYKELDRLFAPERHPDAASLAALDEVKALRKAHVAAFDGAAQQKKRGDVSGAE
                     HRLRREVLPSLAAYIAGIQRLGQLQQQYLEREAQSTQAHIKEARRWVISLCALAVVIG
                     AVVAFLISKSIAEPLRRLTQGSETLSRGEGDADFQGELGSDEVGVLARSMARMAQAEC
                     EMANVARRLCEGDLSVPIRLRGPRDMLGGAMVRLRDTITELQATVQALTESARAGKLA
                     ERAPAQKFNGAFQSLVGGLNATLDALLEPVTLATQKLELLAGGDLSLRMPEHFAGDHA
                     VLSRALNSAAGALDRTIQDVDMSAAQVSTASTQIAAGAQTLAQGASEQVRALDQILES
                     FREIERATSQTSQQASLADRCMREARGSCDRGGEVMRELSEAMVRIRDSSAATARILK
                     TIDEIAFQTNLLALNAAVEAARAGEAGKGFAVVADEVRALAQRSAEAARQTSTLIAQA
                     VSATEDGGRLHSAVAEQLTQIDSRVRETSDAMATVSSAAARQHGNIQNIGRVVDTLNG
                     AIQQVAAHSEEAASAAQELAAQAESLTHVVSQFHVSDSFYVQAKRPTRSSSGGVSQGT
                     RNRADSRGRLSA"
     gene            complement(5974..6423)
                     /locus_tag="VFQ61_12120"
     CDS             complement(5974..6423)
                     /locus_tag="VFQ61_12120"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9955247.1"
                     /translation="MPYSSTPYLYFKGDCEAALEFYERCGLGKVRELRRYEGTPALAR
                     AGAEWTRKVLHSLFDGESRDIQGERVRFYASDGPDSEPMKGCALLIEEAEVERASRLF
                     DELSRGGRVTVAFDLQFWGDYYGNFTDKFGVQWAILTRSPPGRGPHE"
     gene            complement(6544..8643)
                     /locus_tag="VFQ61_12125"
     CDS             complement(6544..8643)
                     /locus_tag="VFQ61_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955248.1"
                     /translation="MSETPERPPNVPRSARWSPSEQVWEQYSLDEFGQVDGEYRAFRR
                     DGSLWFSCVFANGARSGPFQAFHPGGTLAREGQFVEGELDGLIVAYRSDEAGAEQLRP
                     CCVPETARRMHARYERGRQMWERFLDEEGRLLLSDGSLAPTRPEGLPEHASYDELGGR
                     WVCFDSEPSEKNDPSGGDALGTSPGDPAGGLTGTARYFDMNGRLVQEDTYVNDCKLRT
                     RSYWPNGTLRRDESLDSRGRLHGARFVRYGSDEPSPYLDPRIVTEHSEYAHGQPVGIG
                     VFALEGGGELRISRGPACEEVTVLESRAFRAEVQDSKSWLRVAQELEKGGQIREALCV
                     AARAAGTGESRSPLESCLTRYVMPVRTAEASALARSLEQDEPPSISQVLSALLLGAEP
                     GPTLRILSQILPKRLAHAAHDLASAAIVFSAGDFRPYLSRAFRRLELGQVEAALADAA
                     RVSEGSEGLAAFVREYAELLFPKWRFLPAREVIVGSFEGMSESPRQSIDQIRRVIQVY
                     ATRLGRLRSLALAHLPSSSQPDWLPPLLSELLPQGAVALTRYSARIVDTADDGEEISE
                     VHVDESAALELDGLDEIQSRARSEWAALTWLCWAAGLDGVGLPERLGQRQDFSAAASM
                     IVARAARAQDAIQTGGLRARTQGIPGFEWEGYPIDSAPAAFVRLANEEYAELRALFLW
                     LCFAENHSPFQSDLRAQ"
     gene            complement(8836..9429)
                     /locus_tag="VFQ61_12130"
     CDS             complement(8836..9429)
                     /locus_tag="VFQ61_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955249.1"
                     /translation="MFYSKDWIWVAFSLGISASCSGEADFERSSASEDEWSSLAQTLS
                     RDCVGASPDEIIPGLTPSTANGVSRQSPRTYNNRRCYKAFIYQYQDLGPFDHFEAKYV
                     EDSVNLTQSECEARWFGSYLFSDRGTGTWTLECGAFLSGVWNASQGTCKFPGATYGGG
                     PDCKAGRAGWTYKFAGSARMQPTSAAPTAEYVFTASR"
BASE COUNT         1924 a         3232 c         3015 g         1849 t
ORIGIN      
        1 tacagatgag ccccgtcgtc gagccgagcg tcaaggcgtg acgagcaaac cgctagttga
       61 acacagaact tcgataacga gccacgtgac tgtattctgg ttcggtgtcg agcggggatc
      121 gaaatgaggg ggcatcgcgt ccgcggaaac gatgtcactg tgtggccctg cttcgcaaca
      181 cccatcaggc gtgagcctta tccgggctcc taaaacacga ggcaagccgc gcttcgttcg
      241 ctcgagactc gagcgtgcac ccgacgacca cacaatccca ccgtgtgaga acactctagc
      301 tcgcgtgccg tcaaagatcg gacaccaatc gatacactat cgaacacagc gtgttaccct
      361 cacgctgtca tgaacgcgac gggtctcatc gagaccagct ctattcgtcg tgcttaccgc
      421 cggctgttcg aactcggccg cggtgggatg gctcgggtgt acctcgccga gagcctcgcg
      481 tctggggtcc gcaagctggt ggttctgaag atcctcaacc cggaattgtg cgcgaatccc
      541 gagatgcggg cttccttccg ccgggaagcc gagctctcgg cgcagatgaa ccacccgaat
      601 gtggtgcagg tcctggaggt ggtggagtac gccgagaccc cagtgatcgt gatggagtac
      661 ttggacggcg cgtcgctctc ggcgctgctc aagtatgcgg gtaagcacct gtctctgcgg
      721 ttgcgcatgc acattttggc gcaggttttg gccggactgc atcacttcca cgagctccag
      781 gatttggacg gcacaccgct caacaccgtt caccgcgacg tgtcgccgca gaacatcatg
      841 atcctgcacg acgggccggt gaaggtgctg gacttcggga tcgccaaggt caatgcaccg
      901 gacaatcagg tcacgcggac cggcttgatc aagggcaaga tccactatat gccgccggag
      961 cagttgctcg gcgaggccgg cctggatcgc cgtgccgacg tgtttgctgt aggcgtgctg
     1021 ctatgggaag ccgcggcggg gcggcgcatg tgggagggca agaccgaagc ggacgtactg
     1081 cgctgcatga tcacgggcga gctgcccaag gtcgaagact tcgcgcaaga cgtcccggaa
     1141 tcggtgcttc agatcgtgcg gcgcgcgacc catatcgatc gcacgcagcg cttctccagc
     1201 gcccaggaca tgcaggtcgc catcgaacgc ggtctggccg agctcggctg ggcggtacaa
     1261 caaagggatc tcgaggagtt catggcgtgc cacttgggag atcggcgacg tgcgcatgat
     1321 ctcaaggtgc gcgatgcgct gcgtaccccc acgagctcgg acgtgatgat gtactgtacc
     1381 ccggtcaccc aaggctgggc tggccccgag agcggtgtgg atccgtcccg catcactgcg
     1441 gcgggttcgg agcgggcggt ctcacgcgtg tttcgaacca gccgccgttc ctggggctgg
     1501 gcgctcgggc ttttgggcgc gctagctctg ctaggcgctt ggcttgctcg tcgcgaggtg
     1561 cgtcccgcgc aaggcagctt ggcctctccg tccaccgctc cggtgcaccc agagagcgtc
     1621 gaattggagg tggaggcctc tccgcccaat gccgagatct acctagatgg tgagcgcctc
     1681 ggtgttggcc gctattcggg atcgcgccgc ttcgcccccc cgaaggcagt gttggaagtg
     1741 agcgctcctg gttacgcaaa agagcgcaaa gagctttccc tggtccgcga tacggccctg
     1801 caaatcgtgc tcaaaccgga gctcagcgtg caatcaacgc cggtgttcgc agcgagtgca
     1861 gatcccgtaa caggggataa gacagaatcg cccactcgct cggccggacc gcgcacacgg
     1921 cggccaggtg ggtcaacttc cgctcgaggt tcgcgtggct gcaacccgcc ctatacattc
     1981 agcgcggacg gagtaaagac ctacaagcct gagtgctttt gagctgccta ggatcgggcc
     2041 cctcgagaca ccagcaattc tggcatgatc gactcgcgct tggctcgacc actacgaggc
     2101 tttcgctggc tgggcttctg gttcgggtgc ttcgcgtgct cgctgcagat gccctccgaa
     2161 gacgaggtat tcgcgccaaa gaccgcggag acctcggatg gcggcaatac tgcggacatg
     2221 acgggtagca tcggcggctc gagctccatg gggggtgctc cggctggagg caagtcagca
     2281 gtgggcgcca atggcgggcg ctcagtcgat gtcggaggag gctcgtctac gggcggctct
     2341 ggccgaggcg gctcgagcca aggcggttcc acgaatggcg gtgcaaccaa aggcggcgcc
     2401 cacgttggcg gtgttggtgg cgcgtcagag ggtgggtcat cggatggtgg ttcatccgga
     2461 agcgctggcg aagctgggat tggaaccgga ggaggctcgt cggagggcct cgtggcgcac
     2521 ttcgagttcg aagagacgac cggcaccgtc gccgccaata cggtggatca gtcgcgaaac
     2581 gcgagcttcg taggcacttg ttcacacgtg cccggcaagc gcggcaacgc ggtcggcatc
     2641 cgaaacttgg agaatgggac gttggattac gtcgagctcc ccgaagatat ctttagcggt
     2701 ctcaaggcca ctaccatcgc gatgtgggtg cgcgatctct ccaccgcccg taagggcggc
     2761 cgtcttttcg acttcagtcg cggtgcggaa gagacgctct attttgctcc ggatcagccg
     2821 aatcccgaga catcgaccaa tggcgggcac ctcggcggca tccacggcgg ggtatcgttc
     2881 gtggatattt ggttgacgaa atcgttgacc gacaaagcct ggcatcacgt cgccgtaact
     2941 tggagcgcct ccagcatcga cttgtatatc gatggttccc gggccggcgg caaaagcaac
     3001 ccaggagtca ttgcctccga tctcggagcc acttcaacca attggttggg acgcacgctc
     3061 gacgacgcct tcatcgccct ttacgccgag atggacgacc tgcgcattta cgaccgagtg
     3121 ctcagccgag ctgagatctc cagcctatat cgctgaggtc gcgcgacgtg cccctcccgg
     3181 cggcgcgcga gcgccgggag caatgatgca gagcgcgatc aggtttgcct gaggctgctc
     3241 acaagggatc cgcgcgtacc tactcgagtg ctttgaggta aacgagtgtg gcgccccagc
     3301 cgccccgcgc tgccggggcg tctcgaaagg attcaacacg gccgagctcg gccaatagcg
     3361 ctcggatccg ggcgcgctgg gtgcccgtgc cacgaccatg aatcaagcgc acttctcgga
     3421 accctcgcag ctgcgcagcc tctagatagt cgaccactac atcgagaatg tcccgcggct
     3481 gaaaaccatg aagatcgatt gaatcctcga tcggcaagac agcaatagcc tgctcacttt
     3541 ccgagtcatc gggcatggtg cgatcggaat cgtcagcgtc gctcatggtc accgagcagt
     3601 gtactgccgg gagaaccgga gtacccgccg cacactgcac gaagagcgag cgggcgcttc
     3661 gaagcgccga tttcaggcgc tcaaacggcc cctggaatcc gctctgttgc gcgtcccctg
     3721 cgacacgccg ccgctcgagc ttctcgtcgg ccgcttcgct tgaacgtaga aggagtcgct
     3781 cacatgaaac tgagagacga cgtgtgtgag tgactcggct tgtgcggcga gctcttgagc
     3841 cgcgctcgcg gcttcttccg agtgcgccgc aacctgctgg atagccccgt tcagagtgtc
     3901 cacgacccga ccgatgtttt ggatgtttcc gtgctgacgc gccgccgcag aagacacggt
     3961 ggccatcgcg tcgctggtct cgcgaacccg ggagtcgatt tgagtcagtt gctccgcgac
     4021 ggcgctgtgc agccgccccc catcctccgt cgcgctcacc gcttgggcga tcagagtcga
     4081 ggtctgacgc gcggcttccg cgctgcgctg cgcgagcgct cgaacttcgt ccgcgaccac
     4141 cgcgaaacct ttgcccgcct cccctgcgcg ggcggcttct accgcagcgt tcagggcgag
     4201 caggttggtc tgaaacgcaa tctcgtcgat ggttttcagg atgcgcgcgg tcgcggcgct
     4261 cgaatcgcgg atccgcacca tcgcttcgga gagctctcgc atcacctcgc ctccgcgatc
     4321 acagctcccc cgggcctcac gcatgcagcg atcggcgagc gaagcctgtt ggctcgtctg
     4381 cgacgtggca cgctctattt cacggaacga ttcgaggatc tgatcgagag ctcgcacctg
     4441 ctcggaggca ccttgcgcca gcgtctgcgc gccggccgcg atctgagtcg acgccgtact
     4501 tacctgagcc gcggacatgt ctacgtcctg gatcgtgcgg tcgagcgcgc cagcggcgct
     4561 attgagagcg cgtgagagta ctgcgtggtc tcctgcgaag tgctcgggca ttcgcaagga
     4621 gaggtcgcca ccggccagga gctccaactt ttgcgtggcc agcgtcaccg gctctaatag
     4681 agcatcgagc gtggcattca agccgcccac caacgactga aatgcaccat tgaacttctg
     4741 cgccggcgcg cgttcagcga gcttgcctgc acgtgcggat tcagtgagcg cttgcacggt
     4801 ggcctgcagc tccgtgatcg tgtcccgcag ccggaccatg gcgccgccga gcatgtctcg
     4861 cggtccgcgc agccggatcg gtacgctcag atccccttca cacaggcgcc gcgccacatt
     4921 ggccatctcg cactcggctt gggccatgcg cgccatactc cgggcgagta caccgacttc
     4981 gtcgctcccg agctcacctt ggaagtccgc gtcaccctcg ccccgtgaaa gggtctccga
     5041 accttgggta agacgccgga gcggctccgc gatgctcttc gagattagaa acgccacgac
     5101 cgcgcctatc acgaccgcca aagcacaaag ggagatgacc cagcgacgcg cctccttgat
     5161 gtgagcttgc gtggactgag cctcgcgttc cagatactgc tgctggagtt gtcccaggcg
     5221 ctgaatgcct gcaatgtagg cggccaatga gggcaaaact tcgcggcgca accgatgctc
     5281 tgcgcccgac acatcaccgc gcttcttctg ctgcgcagcg ccgtcgaacg cggccacgtg
     5341 ggctttgcgc agcgccttca cctcgtccag ggccgcaagc gaggccgcat ccggatgtcg
     5401 ctcgggggca aacagacgat ccagctcctt gtaggcggag ttgatacctt ctctcgctcg
     5461 cgctacatcg gccgcgtatt gagccgtgag ctcgggagca tcggtcgtga agagtgcgag
     5521 tttcgcggct gcgcctcgat gcaccgcatc tgccaaagca tcactggctc gagccttcgc
     5581 cgggacctcg cgtgtagcaa tctgcagcac cgcgccgagt gcttcgaaac gtttgatggc
     5641 gaagacaccc gtcgtgatca tcagagtgat gaacaaggcc gaaccgagcc acaaacgtgt
     5701 acgcacggaa accacccgca tccagtgatc tcgttgaacg ttccgtagct cggtcatccc
     5761 ctcaaatagc ggtcgcgggg cccctcgggt taagcggccg gcgagcccag catggcagcc
     5821 gccgtgcgcg acgacctcac cgggctcgtt gatctctgcg gtgcgaaccc agtgctcaac
     5881 actgggcgtg tcgtccagaa cacggacatg ctcgcgacgg gtaataaggc tcgagtccac
     5941 cgcgacatct cacgatcggc tatccgagcg gtcctattcg tgaggtcccc ttcccggagg
     6001 cgagcgggtc agaatcgccc attggactcc aaacttgtcc gtgaagttcc cgtagtaatc
     6061 cccccagaac tgcagatcga acgccaccgt gacgcgcccc cctcgagaca gttcgtcgaa
     6121 tagtcgcgac gcacgctcga cttccgcctc ctcgatcagc agcgcgcagc ccttcatggg
     6181 ttcggaatcc ggtccgtccg atgcgtaaaa ccgtactctt tcgccctgga tgtctcgcga
     6241 ctcgccatcg aaaagtgagt gcagcacctt gcgcgtccac tcggcgccag cacgcgcgag
     6301 cgcgggcgta ccctcgtaac gcctcagttc gcgaaccttg cccaagccgc agcgctcata
     6361 aaactcgagc gcggcttcgc agtcgccttt gaagtataag tatggtgtcg atgagtatgg
     6421 catcgcccga gatcacctcc tgcagctcgc acccgcgaat gcctgaatgc ggtcctcgac
     6481 caaagcgaac ggaatgccta tagcgagctc agccctcgcc atcaactaca aatgtagtcc
     6541 aactcactgc gcgcgaagat cgctctgaaa tggcgaatgg ttctcggcaa agcagagcca
     6601 cagaaagaga gctcggagct ctgcgtactc ctcgttggcg agccgtacga aggccgctgg
     6661 agcactgtcg atcggatagc cctcccattc gaaaccgggt attccctgcg tgcgcgcccg
     6721 cagcccaccg gtctggatcg catcctgcgc ccgtgccgca cgagcaacaa tcatgctcgc
     6781 cgctgccgaa aagtcctggc gctgcccaag ccgctcgggc aagcccaccc cgtcgagccc
     6841 ggcggcccag cacagccaag tcaacgcggc ccactcggag cgagcgcggg attggatttc
     6901 atctaggccg tccagctcga gagcggcgct ctcatcgacg tgcacctcgg aaatctcctc
     6961 accgtcatct gccgtatcca cgattcgggc ggaatagcgc gtgagcgcca cggcaccctg
     7021 cggcaacagc tcggaaagaa gaggcggtaa ccaatctggt tgcgagctgc tcggcaagtg
     7081 cgcgagcgcc aaagacctca gccgcccgag cctcgtggca tacacttgga tcacccggcg
     7141 aatctgatcg atggattgcc tgggactctc actcataccc tcgaagctcc cgacgatcac
     7201 ctcgcgcgct ggaagaaagc gccatttcgg gaagagcagc tcggcatact cacgcacgaa
     7261 ggcagccaac ccctcggagc cctcgctcac ccgtgccgcg tcggccaacg cggcctcgac
     7321 ttggccaagt tctagccgtc gaaaggcacg gctcaggtac ggtcgaaagt cccctgctga
     7381 aaaaacaatc gcggcggacg ccaagtcatg agcagcgtgt gccaagcgct tgggcaaaat
     7441 ttgagacagg atgcgcagcg tgggcccggg ctctgctccc agcagcaaag ccgatagcac
     7501 ctgggaaatc gaaggcggct cgtcctgttc caggctccgt gccagagcgg acgcttcggc
     7561 ggtcctcact ggcatcacgt accgagtcaa acacgactcc agcggtgagc gggactcacc
     7621 cgtacctgcg gcacgcgcgg ccacacatag agcttcgcgg atctgcccac ctttctccag
     7681 ctcttgtgcg actcgaagcc acgacttgga atcttgaacc tccgctcgga acgcgcgcga
     7741 ctcgagcacg gtcacctcct cgcacgccgg ccctctcgaa atccgaagct cacccccacc
     7801 ctcgagagca aacacgccaa tgccgactgg ctggccatgt gcatactcag agtgttcggt
     7861 tacgatgcgc gggtcgagat acgggctcgg ctcgtctgac ccgtagcgga cgaatcgggc
     7921 gccgtgcagg cgccccctcg agtccaggct ttcgtcgcgg cgcagcgttc cattcggcca
     7981 atagctgcgc gtgcgcagct tgcagtcatt cacgtaggtg tcctcctgaa cgagcctgcc
     8041 attcatatcg aagtagcgcg ccgtgccggt caggcccccc gccgggtcgc cggggctagt
     8101 gcccaacgca tcgccaccag aaggatcgtt cttttcggag ggctccgaat cgaagcatac
     8161 ccaacgcccg cccagctcat cgtaagaggc gtgctcgggc aagccctccg ggcgggtggg
     8221 agccagggag ccgtccgaga gcagcaagcg gccttcttca tcaagaaagc gctcccacat
     8281 ctgacgccct cgctcgtacc tggcgtgcat tcggcgcgcg gtttcgggca cgcagcaagg
     8341 gcgcaattgt tccgcgcccg cttcgtccga tcgatacgcc acgatcagcc cgtccaactc
     8401 gccttctacg aactggccct cccgagccaa cgtgccgccc gggtgaaagg cctggaaggg
     8461 acctgaccgg gctccgttgg caaacacaca cgaaaaccaa agtgatccgt cccgccggaa
     8521 ggcacggtat tccccgtcca cttgtccgaa ctcatcaagt gaatactgtt cccaaacctg
     8581 ctcgctcgga gaccatctcg ccgatcgcgg cacgttcggg gggcgttcgg gggtttcgct
     8641 catgacttgc tgcggaggcg actgcgctag tcggagcgag agcatagcaa gcaatggctc
     8701 ttacactggc ccttcgggtt gaaactgttt caactggcgg gttctaggat cacccgccgg
     8761 gcgagctccg ggtcgttctt cggtctcggc tattttcggt tcacgctgct agcggagctc
     8821 agcaaagatg cgcagtcagc gtgacgcagt aaatacgtac tctgcagtcg gcgctgctga
     8881 ggtgggctgc atccgggcgc tgcccgcaaa cttgtaggtc cagcccgctc ggcccgcctt
     8941 gcagtccgga ccaccaccgt acgttgcacc cgggaactta caagttccct gactggcatt
     9001 ccaaaccccc gacagaaagg cgccacactc cagcgtccaa gttcccgtcc cacgatccga
     9061 gaacaagtac gaaccaaacc agcgcgcttc gcactcggat tgcgtcaaat tgacggagtc
     9121 ctcgacgtac ttggcctcga agtgatcgaa gggtccgagg tcctgatact gataaataaa
     9181 agccttgtag caacggcggt tgttgtaggt gcgcggtgat tgtcgggaga ccccgttcgc
     9241 agtggacggt gtaagaccag gaatgatctc gtccgggctc gcgccgacgc aatcccggct
     9301 caaggtttgc gcgaggctac tccattcgtc ttccgaagcc gagctccgct caaaatcagc
     9361 ttcaccagag caactcgccg agatccccag cgagaacgcg acccaaatcc aatcttttga
     9421 atagaacatg cttcaatctc caaatatgaa ttgaacgata gaacgagtgc agcaacgcac
     9481 ccgtagcgct cccctcatcg accgattcga cacttctcgc agaccaactc accatatccc
     9541 acgccatcga gacagcgtgc gatgtgcgac atccgcgatt gatcgctaaa actgcgagtt
     9601 ggtcgccagt cggcttgcga ttggtcgccg agtggattgt gaaactggac tgaccaatta
     9661 gtatcgcgaa actcgctgcg tcggcgctct cgaacgaacg gcacggccgc tagcccagca
     9721 gaggctccag aagcagccgg cgctacggat tcgcattctt aggcactcgc tcacgcgagc
     9781 cgaatcaacc ctcaagttat tcttgcaacc ttaagataga gagtcactcc tgtcaaactt
     9841 gattacaagg ttcgatgacg gacaaggttc gatgacggac aaggtttgat gacgaccttg
     9901 cgctgctgac actgctgctg accgcgctct gctccgctca actgacggct cttcgctcga
     9961 ctgatggctc ttcgctcgac tgatggctct tcgctcgact gatggctctt cgctcgactg
//
LOCUS       DASTKU010000349         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_67273,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000349 DASTKU010000000
VERSION     DASTKU010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67273"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1383
                     /locus_tag="VFQ61_12135"
     CDS             205..1383
                     /locus_tag="VFQ61_12135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HET9955250.1"
                     /translation="MTSTTNCAPPPPRSLAELLTFDDSKLARAWERGRVPISDLYEAY
                     IDGRVDIPSDSWDTLFASRHALLSFRLTQSHVKWAFTNFLPEVALHSLRQDRRIVSEH
                     YDRGNDFFNWFLGESMVYTAASFESPSMGLEEAQYRKIDRCCEKLQLGPGQTLLDIGC
                     GWGTFVARAARDFGVRAVGVTLAREQVEFGTERIRAYGVTGRAQVEVQDYRAVSGRFD
                     KIVSLEMVEHVGVKNLSRFFQKVSELLSDDGLFVLQWTGIRRLYQPQNPLSALTLAPE
                     DLMWALFMNRYIFPGADASLPLSSMLRAAEDAGFEIEDVERMSAHYILTLRAWRERWI
                     ANRAAVVAKYGERWHRLWSFFLYWAALMGEEGAGFTYQVVMHKNRVRFPRLELEFGKR
                     "
     gene            1881..>3088
                     /locus_tag="VFQ61_12140"
     CDS             1881..>3088
                     /locus_tag="VFQ61_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955251.1"
                     /translation="MFSKLASSKLAPSMVKRNLARTRPARFPLATVLGSIGVLACGQI
                     YEDPTPGSGGTSGGTGGKTGVTLTGGAGGGSSGSGGSGGAGRGGTAGTTGITLVTGGS
                     SSGGRTSAGGAAGQGAAPSGGSAGKGGAGGAGGTTVAGGSIGWAGAGGDWGDVGDYVA
                     LRESSWNVRLAMNVAPVSTYPVSCPKAEFTLHVKPVGDTLEVISGRNGSVMTGQLLRT
                     PRLETTYGLGDQLGFPTRGECTFSTIGIKEIALWGWDENADGQADSIAGEGKAQAMFI
                     MGDVGITVQMTFRLTGNPDTAPPTILPPSELHPLDGVMLTASEPLSLKSGAGLNSETG
                     AARVPLTANVTDDAAIGSFSSARILDFGSRWRVTANGSDLAGLPFDQALTPSAVVLSD
                     PGVFAQDGFEG"
BASE COUNT          561 a          874 c         1059 g          594 t
ORIGIN      
        1 ccgagctctt tccgagctcc aggcgctcga gcaattggtt ataggacgtc ccgtggaagc
       61 cgttgcgggc aaactccgcg gcggccactc gaaggatcgc ctcctgacgg tctgatggaa
      121 gccgctcgaa gcgtcgccgg gccacggtgc cttgctagca caaacatcac tgtactgtcg
      181 gtacaaccaa tggtacagga cggtatgacc tcgacgacga attgcgcgcc gccacccccc
      241 cgaagcctgg ctgagcttct caccttcgat gactcgaagc tggcccgcgc ttgggagcgc
      301 gggcgggtgc cgatctcgga tctctacgaa gcctatatcg acgggcgagt ggacattccg
      361 agcgatagct gggacacgct cttcgcctcg aggcacgcgc tactctcttt tcggctcact
      421 cagtcgcacg tgaagtgggc cttcacgaac ttccttcccg aggtcgccct tcactcgctg
      481 cggcaggatc gtcggatcgt gagcgagcac tacgatcggg gtaacgactt cttcaattgg
      541 ttcctcggtg aatccatggt gtacaccgcg gcgtcattcg agagtccgag catgggcctc
      601 gaggaggcgc aatacagaaa gatcgatcgc tgctgcgaga aattgcagct cgggcccggg
      661 cagactttgc tcgatatcgg atgcggctgg gggaccttcg tggcgcgtgc tgcccgcgac
      721 tttggagttc gggcggtcgg ggtgacgctg gctcgagagc aggtggagtt cgggacggaa
      781 aggatccgcg cctacggcgt gacggggcgc gcgcaggtcg aagtgcaaga ctatcgcgca
      841 gtgagcggac gcttcgacaa gatcgtaagt ctcgagatgg tcgagcacgt aggcgtgaag
      901 aacctctcgc gcttctttca aaaggtctcc gagctcctca gcgatgacgg gctgttcgtg
      961 ctgcaatgga ccggcatccg ccggctctac cagccgcaga atccactcag cgctctcacg
     1021 cttgcgcccg aagatctgat gtgggcgctg ttcatgaacc gctacatctt ccccggagcc
     1081 gacgcgagcc taccgctctc cagcatgctc cgggcggccg aagatgcggg cttcgagatc
     1141 gaagacgtag aacgaatgag cgcacactac attctgacgc tccgcgcttg gcgcgagcgc
     1201 tggatcgcca accgcgcggc ggtcgtggcg aagtatggcg aacgctggca tcggctgtgg
     1261 tccttcttcc tctattgggc ggccttgatg ggcgaagagg gggcaggctt tacttaccag
     1321 gtggtgatgc acaagaaccg cgttcgcttc ccacgattgg agttagagtt cgggaagcga
     1381 taattcccgc gctctcggcg tgggttactc gagagctgcc gcggcggtga cggatcggag
     1441 agatgggtgc gaggaggaga ggacgaggca agtaaacgag gcaagtagac actctcgact
     1501 cgtcccgctc aaccaagtga gatctggttc ggcattctcg cgcgctcggc ccttcggctc
     1561 cgctcctttg ctggcatgcg cgagctagct gctgcaatct acggttcggg aaactcatgg
     1621 attggtgatc tccgcaccca atggacttac tccggttggc cttcaaatcc cgatcgtgaa
     1681 gagcgtcgct agttttggga cgtggcattc gtcgcgctcg tttggaaacg gcccaaactt
     1741 cgaggagtaa gctcgagccg ccgcctttgt ggcgcgagct tgtgccgagt tgtgccaagg
     1801 gcgcgagttg cttcggattt attcgcgtga aagtggcctg ttgagcttgg cgtaaagttt
     1861 gctgtaactg tcggcatcgc atgttcagca aactcgcgtc cagcaagctc gcgcccagta
     1921 tggtgaaacg caacctcgcg aggacccggc ccgctcgctt tccgcttgcg acggtgctgg
     1981 gctcgattgg ggttctggct tgtggccaga tttacgagga tccaacgcca ggcagtggcg
     2041 gcaccagcgg cgggacgggt gggaagacgg gcgttacttt gacgggagga gcgggcgggg
     2101 gcagctcagg aagtggagga agtggaggcg caggccgcgg cggaacggcg ggaaccacag
     2161 ggatcacgct ggttacgggc ggtagttcct ccggcggaag gacgagcgcc ggaggtgccg
     2221 ccggacaggg cgcagcgccg agcggcggaa gtgcgggcaa gggcggcgct gggggcgctg
     2281 ggggcacgac ggtcgcaggc ggcagcatcg gctgggcggg agccggtgga gattggggag
     2341 acgtgggcga ttacgtcgct ttgcgcgaga gctcttggaa cgtgcggttg gccatgaatg
     2401 tggcgcccgt ctcgacctat ccggtgtctt gcccgaaggc agagttcacg cttcacgtga
     2461 agcccgtcgg ggacactctc gaggtgatct cggggcgcaa cggcagcgtg atgaccggac
     2521 aactcttgcg cacgccgcgg ctcgagacga cgtatggttt gggggatcag cttggttttc
     2581 ccacgcgcgg agagtgcact ttcagtacga tcggcatcaa agagatcgcg ctctggggtt
     2641 gggacgagaa tgcggatggg caggccgact cgatcgctgg cgagggcaaa gcacaagcca
     2701 tgttcatcat gggtgacgtg ggcatcacgg tgcagatgac gttccggctg acgggcaatc
     2761 cggataccgc gccgccgacg attttgcccc cgtccgagct tcatcccctc gatggcgtga
     2821 tgctcaccgc gagtgaacct ttgagcttga agagcggcgc ggggctgaac agcgaaacgg
     2881 gagcggcgcg agtcccactc actgcgaatg tcacggacga cgcggccatt gggtcattct
     2941 cgtcagcaag aatcttggac tttggtagcc gttggcgcgt gaccgcgaac ggttccgacc
     3001 tggcagggct gccgttcgat caagcgttga ccccgagcgc ggtcgtgctg tccgatccgg
     3061 gagttttcgc tcaggacggc ttcgaagg
//
LOCUS       DASTKU010000350        13395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_735594, whole genome shotgun sequence.
ACCESSION   DASTKU010000350 DASTKU010000000
VERSION     DASTKU010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13395
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735594"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..820)
                     /gene="map"
                     /locus_tag="VFQ61_12145"
     CDS             complement(53..820)
                     /gene="map"
                     /locus_tag="VFQ61_12145"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET9955252.1"
                     /translation="MHLAMAIEILSPFEIEHMRRAGRAAAATLAYVGSQLRPGMSTAE
                     IDRLVREDTLRRGGRPSQLGYQGFPAAVCTSRNQVVCHGVPSPHEVLVPGDIINIDVT
                     TNLDGYHGDTSAMFTIGEVRPEAQFLVDVARRCRDAGIAVIRDGARLGDIGAAIGEVA
                     NQEGVSVVTELGGHGIGRVMHAAPHVPHTGRKGAGIRLRAGMALTVEPMVNLGKAATT
                     TLKDGWTVVTADSTLSAQWEHTVVVTREGHEIMTVSE"
     gene            906..1244
                     /locus_tag="VFQ61_12150"
     CDS             906..1244
                     /locus_tag="VFQ61_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9955253.1"
                     /translation="MQTGIVSLSQLRYFVAVAEEGHVGRAAHRLHVAQPPLSRQIRAL
                     EDEVGAALFTRTPRGMTLLPAGQVFLDHARAILSAVDRAVEAVRFEQDSATAQSAPLT
                     RPRASGRAAL"
     gene            complement(1207..5571)
                     /locus_tag="VFQ61_12155"
     CDS             complement(1207..5571)
                     /locus_tag="VFQ61_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET9955254.1"
                     /translation="MSQLSADKLPAGSLAPSAVVGLGASAGGLDALSEFFASTPPFTG
                     MAFVVVQHLSPDYKSLMPELLARHTAMPISRVQHGMRIEPNTVYLMPAQRDMSVVDGR
                     LHLQDRAEGGLHHPIDFFFCSLAALGERAVGIVLSGTGSDGTRGCKEIHRRGGLVLVQ
                     EPGTAAFDGMPASAIEGGKPDHVLSPQRMAQVILERVGEDVGARVNEVRESAALERLI
                     GLMSQVTQIDFSIYKPATLVRRSQRRMQELGLQDWDAFVEFMQQHSPEVERLAHEALI
                     GVSSFFRDAAAFDFLQSEVLPHLLKTDSFDGSLRAWCAACSTGQEAYSLAITAFDVAE
                     SLSVPLKLKLFATDVSPRAIELASSGVYSRELDKQVRSERLSRYFQPCRAGYQVVRSL
                     RQAVVFSVHNLLRDPPFSRLDLVSCRNLLIYLQPHAQRNVLTRLALALRPGGILFLGS
                     SENIGDLGDSFEALDARGRIYRRKPTALRSYVSTSGFYSTRRAANAEPQEQRRLIEEA
                     YRTLVQLDSPVSVIINDQNRIVHTTGDAGEFFALPVGDASLDILHLARGNLSTLLSAA
                     IAKGRRDQRAVEYADVDLRLGTERARLDLRVVPLGDSAAGRHQLVSIRKHAAEATRSR
                     DSDVLVGDREQLDALQNELSSTRENLQAAIEELETANEELQASNEELVASNEELQATN
                     EELQATNEELLTANAESERRIQDLVELNADMENLMRSTNVGTLFLDEQLDVRKFNPPV
                     CEFLSLLERDLGRPVAHIRHGLQGVELARLAQHVLESGASEQVPATCGERHVLLRAVP
                     YQLRQATRGVVISFIDVTELSVARAELEGVMDCLPEQIAVLDANGVIVRVNSAWRAFA
                     AQNGCAERAASPGVSYLDVTTRACAEQPEAERVLVGLREVLAGRRERFTHEYRCDSPS
                     EERHFILDATRVADAGAVISHKNVTPIARAAQQAQNSAALYRLLFAMSQEALALIAPD
                     SGAVVEANPAMAELAGRTADELVGIDLGRLLRPVESGWEPLLSSALRNRSVDGLSVLI
                     GYGNTERAAKVSLSALEVDQRRLVLLRLCEPVTERLLDERAALRSRLSQAQKMEALGL
                     LAGGVAHELNNVLTAILGVAYARQAELDSKDPLAQDFDIVRAACNRGADLTRNLLGFA
                     RAGSVAREHFELADVAKEVVSLVRRNAPPNVTIALRIGDSCFVRGDRSQIAQALMNLC
                     INAVDAVRDAGTVEVEVKCGRLRDGTAQAGEFSFLRVIDNGIGMDEGTRDHAFEPFFT
                     TKARGVGSGLGLSMVYGVSKSHGGFAEIESTPGKGTVVTFALPKAQSPSKTEADSTPA
                     RGAGEHVLVVDDEEMVRRAMARLLDRHGFIVESVNSGREALNRLKSPDELPVELVMLD
                     IVMPELDGLGTLEAIRALYPTLPVLLYSGYPDRAPHKILEIDPHTAFCTKPVDPGHLT
                     KTMLLLLKGQRGPMRVGV"
     gene            complement(5911..6594)
                     /locus_tag="VFQ61_12160"
     CDS             complement(5911..6594)
                     /locus_tag="VFQ61_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9955255.1"
                     /translation="MRSLPPLYVSALAETGRECAKGVSSNTTSSVGLDVCGGGACADV
                     HGAVLDLGRRGECDWGAAAPRLSKRLFGAVEPEGRVCGGAASDPKTQELTGYLSATGV
                     GELLDAPIYRDGAMIGVVCHEHCGGTRRWTEMEASFASAVADTLTNFDRTSGAGGAAR
                     SAGGERQAEARMEKMQALQRLARAVAHDLNNVLTQSRSFMSARMLCPCRVMKFLLVSM
                     ATTDFVTLA"
     gene            6660..7271
                     /locus_tag="VFQ61_12165"
     CDS             6660..7271
                     /locus_tag="VFQ61_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9955256.1"
                     /translation="MGEVYERLLRAIARNDDEIRFAVRCGSRVYGTATPASDEDFFVV
                     LRDREAKRDLVWVDHMNVVIHGTGAFEAALAEQSVFALECLCTPAEHRLKEATPRFPL
                     EVRRARLFDSVKDRSDSDFKKALKRYDEDPAAARKRVFHALRVLSFARQLLEAGRITD
                     FSAANGWWEELADATELTPQDIERRFGSIREELITQLGALTRR"
     gene            complement(7303..7956)
                     /locus_tag="VFQ61_12170"
     CDS             complement(7303..7956)
                     /locus_tag="VFQ61_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HET9955257.1"
                     /translation="MSAKYPRSFHLPWSPGGTSDDRRLLDVSGLIDVEIVITEKCDGS
                     NLTYTRDHVFSRSHAGPPGHRSFDLAKATHARLAHLISEGLSVFCEYCYAVHSLVYEA
                     LPDYSLVFGVRDDVRGMWWDWDMVAAQALELGLPTVPLLFRGRVSDESALHELTEALS
                     NETSAFGGMREGVVVRRAGEFEDSQFSRSLAKWVRRDHVTTDEHWMHQVIQVQRLRE"
     gene            8019..9134
                     /locus_tag="VFQ61_12175"
     CDS             8019..9134
                     /locus_tag="VFQ61_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9955258.1"
                     /translation="MLDELLSPTPPEYRVDWESLQAFEWVRALEACPQDPIHHAEGNV
                     WIHTRMVLETLAEMAAWRALPPGDQHAVYLACLLHDVAKPATTRIEEDGRVTAKGHSR
                     RGEVLARRLLWELGVPFATRELVCGLIRYHQLPFYLIERPDAQRLTAEISLVARCNLL
                     GIVAEADIRGRLCSDLPRILDNIALYREFCAEEGCLAKPRAFASAHTRVQYFRADGRH
                     PDVEVWDDTRSDVVVMSGLPGSGKDTYIRENFPEWPIVSLDGLREELDVDPTDNQGPI
                     VQLARERAREHLRRGERFVWNATNICKRFRGPVLSLMADYHARMHVIYVESPAVRLFS
                     QNRNRPAMVPEKAIRGMLERWEIPDLTEAHEVTYIAE"
     gene            complement(9176..9949)
                     /locus_tag="VFQ61_12180"
     CDS             complement(9176..9949)
                     /locus_tag="VFQ61_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9955259.1"
                     /translation="MHTAGRRGATRVVSFGLMRTPAPTPDDPTVYPVEEPVGEEILQC
                     WMLEVLRPLIERWLAERGTPCFVGSDQFIYFEKGQVSQRIAPDIYVLPDVPQDLDIPS
                     WKIWESGIHPSFALEICSQDWRKDYEVAPRLHHRIGTEELIVVDPDYLKRREGYRLQR
                     FERTSRGLELQERTQADRVRSRVLGCFVRLVGTERPRQRVRLALDPDGDILFPTDSER
                     AEEQRQRADEQRKRADAERQRAEAALARIAELEALLERR"
     gene            10930..12027
                     /locus_tag="VFQ61_12185"
     CDS             10930..12027
                     /locus_tag="VFQ61_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955260.1"
                     /translation="MSIRNLKAFGLGSLLLMAFGCSAEAGENFESQESQSTQQAIVVS
                     PQKLHVVGVTSSGGLRHALRTGTSWSGFGDVEGQAGDIGTVTDAQAEISLGGLHVIAI
                     AGGALFHTIRSETSWQPFVNMAVPLGTVGTYNSVALANGTAGTHVCVTTTAGGLFHTI
                     RSSTSWQPWGDVKLASNSNPGSLKRVACSVHSVVVGQVPAPPGGISLPIFGDELHVVA
                     LTTSGVPYHAIRHPNGSWDTLNNVNVPTGNTTPFSDIDIDVDPNGELHVVGTGSNLQY
                     HSFRTSGGWSVFGDIEQPAGDPGNQQSGAAVALDEGLYVFDQLSNGGLMMTRRGSGAW
                     DAFMDVRVATGSTESFKKVSTAGESAVIGPN"
     gene            12416..13261
                     /locus_tag="VFQ61_12190"
     CDS             12416..13261
                     /locus_tag="VFQ61_12190"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HET9955261.1"
                     /translation="MNHQPTPGGGAPAHTERAAGSVLSPAPLSSVAAPTPTRGLELTE
                     LKGLELDGMHVDGVAIELDLASAVLEPRAVPDRRLTSLAADPTFWVAVNAGYFERDGS
                     PSGLLASSARRYGLLDRRGGSGIVIVSAERAQLVPFEGFPSNSFRGELAVQSGPRLIE
                     PNGSLGMRSDDGQRAPRTVLCVMHQGRSLALVLLWSREEPARGPGLMTVARLLTGPSP
                     RGSERWCEQALNLDGGPSTGIVYSRAFTRRVTPEHLPLGPVPWALVARPRVGTANPSS
                     APPQP"
BASE COUNT         2588 a         4384 c         3976 g         2447 t
ORIGIN      
        1 gaagcccagg attcggaagc ccgaattcag agactcgaat cgtgggtcaa ggctactccg
       61 atacggtcat gatttcatga ccttcgcggg tcaccacgac cgtgtgctcc cactgcgcgg
      121 atagtgtgct gtcggcagtc acgacggtcc aaccatcctt caaagtcgtc gtagcagcct
      181 taccgaggtt caccatcggc tccaccgtga gtgccatgcc agctcgcaag cggatgccag
      241 cgcccttgcg acccgtgtgc ggaacgtgag gcgcggcgtg catgacccgg ccgatgccgt
      301 gtccaccgag ctccgttaca acgctgacgc cctcctggtt agctacctct ccaatcgccg
      361 cgccaatgtc tccgagacgg gcgccatctc taatgactgc gatccctgcg tcacgacagc
      421 ggcgcgcgac gtccacgaga aactgtgcct caggcctcac ctcgccaatc gtgaacattg
      481 cgctggtgtc cccgtgatag ccatcgaggt tcgtcgtcac gtcgatgttg atgatgtcac
      541 ctggcacgag cacctcgtgc gggctcggga cgccgtgaca cactacctgg ttgcgactcg
      601 tgcaaacggc agccggaaag ccctgatatc cgagctgact gggccggcct ccacgccgca
      661 gtgtgtcttc ccgaaccagg cgatcgatct ccgccgtcga catacctggg cgcaactgcg
      721 agcccacata cgccaatgtg gccgcggcag cgcgaccggc gcgccgcatg tgttctattt
      781 cgaacgggct caggatctcg atcgccatcg ccaagtgcat gaaccagccc tgggtgggcc
      841 gtccaatacc ctttcgatct cgaccggatc ccgttaaacc tcccttgcaa ggagcggggg
      901 gcgcaatgca aactggcatc gtgagcctgt cgcaacttcg ctatttcgtt gccgttgccg
      961 aagaggggca cgtcggccgc gccgcgcacc gcctccacgt ggcgcagccg cccctcagcc
     1021 gccaaattcg cgcgctggaa gacgaggtcg gcgcggcgct cttcacgcga accccccgcg
     1081 gcatgacgct gctccccgcg gggcaggtat ttctcgacca cgcacgagcc atcctctccg
     1141 cggtggaccg cgcggtcgaa gccgtgcgct tcgagcaaga tagcgcgacg gcccagagcg
     1201 ctccgctcac acgcccacgc gcatcgggcc gcgctgccct ttgagcagaa gcagcatcgt
     1261 cttcgtgagg tgtcccggat cgaccggctt ggtgcaaaac gcggtgtggg gatcgatctc
     1321 cagaatcttg tggggtgccc ggtctgggta tccagagtag agcagcaccg gcaaggtcgg
     1381 ataaagagcg cggatcgcct cgagtgttcc caggccatcg agttcgggca tcacgatgtc
     1441 gagcatcacg agttcgacgg gcagctcatc gggggacttg agccgattga gtgcctcgcg
     1501 tccgctattg acgctctcca cgatgaagcc gtggcgatcg agtagccgcg ccatggcgcg
     1561 cctcaccatc tcctcgtcgt cgacgaccag gacgtgttca cccgctccgc gtgcgggagt
     1621 cgagtccgct tcggttttgg acgggctttg tgcctttggc agagcgaagg tcacgaccgt
     1681 acctttacca ggcgtactct cgatctccgc gaagccacca tgagacttgc tcacgccata
     1741 caccatcgat aggccgaggc cgcttccgac cccgcgtgcc ttggtcgtga agaacggctc
     1801 gaaagcatgg tcgcgcgtcc cctcatccat gccaattccg ttgtcgatga cgcgcaagaa
     1861 cgaaaactca cccgcttgcg cagttccatc ccgcaaccgc ccacacttga cctcgacttc
     1921 cacggtccct gcgtcacgca cggcatccac ggcattgatg caaagattca tcaaagcttg
     1981 agcgatttgg cttcgatctc cgcgcacgaa acaactatct ccgattcgca gcgcgatcgt
     2041 gacgttgggg ggggcgttgc ggcgcaccaa gctcacgact tccttcgcca cgtccgcgag
     2101 ctcgaagtgc tcccgagcca cgcttccggc gcgggcaaac ccgagcagat tgcgagtcaa
     2161 atcagcgccc cgattgcacg ccgcccgaac gatgtcgaag tcctgggcga gcggatcttt
     2221 cgaatccagc tcggcctggc gcgcgtaagc gacgcccaaa atcgcggtca acacgttatt
     2281 gagttcgtgg gccaccccac ctgcaagcag acccaaagct tccatctttt gggcttggga
     2341 caagcgcgag cgcagcgccg ctcgttcatc gagaaggcgt tccgtgactg gttcgcacag
     2401 ccgcagcagc accagcctgc gctggtccac ctcgagcgcg ctaaggctca ctttggccgc
     2461 tcgctccgtg ttgccgtaac cgatgagcac cgagagccca tcgaccgagc ggttgcgcag
     2521 ggccgaactg agcagcggct cccagcctga ttccaccgga cgaagcaggc gcccgagatc
     2581 gatacccacg agctcgtccg cggtgcgtcc cgcgagctcg gccatggccg ggttggcttc
     2641 caccaccgcg ccgctgtccg gcgcaatcag ggccagcgct tcttgactca tggcaaacag
     2701 cagccggtag agtgccgcgc tgttctgcgc ctgctgcgcg gcgcgggcaa ttggcgtcac
     2761 gttcttgtgc gagatcaccg ctcccgcatc cgccacccgc gtcgcatcca agatgaagtg
     2821 gcgctcctcg cttggagaat cacaacgata ctcgtgcgtg aaacgctcgc gacgccccgc
     2881 caacacctcg cgcaggccca ccagcactcg ttcggcctcc ggttgctcgg cgcaagcacg
     2941 ggtggtgaca tctagatacg acactccagg gctggccgcg cgctccgcac acccgttctg
     3001 cgccgcgaac gcgcgccagg cgctattcac tctaacaatc acgccgtttg cgtccagcac
     3061 ggcaatttgt tctggcaagc aatccatcac gccttcgagc tccgcgcggg ctacggaaag
     3121 ctccgtcacg tcaatgaagc tgatcacgac gccgcgcgta gcttgccgca actgataggg
     3181 cacggcccgt aacaacacgt gccgctcacc acacgtagcg ggcacttgtt cactcgctcc
     3241 actctccagc acgtgttgag ccaatcgcgc cagctccacc ccttgcagtc catggcggat
     3301 atgagcgacg ggccgcccca gatcccgctc gagcagagaa aggaactcac acacgggcgg
     3361 gttgaacttg cgaacatcca gctgctcgtc gagaaagagg gtgccgacgt tcgtactccg
     3421 catgagattc tccatgtcgg cgttcaactc gaccagatcc tgaatgcgcc tctcgctctc
     3481 cgcgttggcg gtcaacagct cttcgttggt cgcctggagc tcctcgttcg tcgcctgcag
     3541 ctcctcgttc gatgcgacca gctcctcgtt gctcgcttga agctcttcgt tggcggtctc
     3601 cagctcctcg atcgcggcct gcaggttctc tcgagtcgag ctgagctcgt tttgaagcgc
     3661 gtccaactgt tctcggtccc ccacgagaac gtcgctgtct cgagatcggg ttgcctcggc
     3721 cgcgtgcttt cgaatgctga ctagttggtg ccggcccgcg gcggagtctc cgagcggtac
     3781 gacccgcagg tccaaacgcg cgcgttcggt gcccagccgc aggtcgacgt cggcgtactc
     3841 gaccgcgcgt tggtcgcgtc gtcccttggc gatggcagcc gagagcagcg tcgataggtt
     3901 accgcgagcc aaatgcaaga tatccagact cgcgtccccc accggtagcg caaagaactc
     3961 gcccgcatcc ccggtcgtgt gcacgatccg attctgatcg ttgatgatta ccgacaccgg
     4021 cgagtccagc tgaaccaaag tacggtaagc ctcttcgatg agtcgtcgtt gctcttgcgg
     4081 ttcggcgttc gccgcccgcc gcgtagaata gaagccggac gtagagacgt aggagcggag
     4141 ggcggtcggc ttgcgccggt agatgcgccc ccgagcatcc agcgcctcga atgagtcccc
     4201 cagatcgcca atgttctcgc tgctgccgag gaagagaatt cccccgggtc gcaacgccag
     4261 cgcgagccgc gtcaaaacgt tccgctgcgc gtgcggctgc aagtaaatca gtaaattgcg
     4321 acaactcacg agatccagcc gcgagaacgg ggggtcgcgg agcaggttgt gcacgctgaa
     4381 caccacggct tggcgcaggc tccgaactac ctggtaaccg gcgcgacacg gttggaagta
     4441 ccgggacaaa cgctcactgc gcacttgctt gtcgagctcg cgtgaataga ctccgctact
     4501 cgcgagctcg atcgcccgtg gggacacgtc cgtcgcgaac agcttcaact tgagaggaac
     4561 actcaagctc tccgccacgt cgaaggcggt gatggccagt gagtaggctt cttgcccagt
     4621 ggaacaggcg gcacaccagg cgcgcagaga gccatcgaag ctgtcagtct tcaggaggtg
     4681 cgggagtact tcgctttgca aaaaatcgaa agccgctgca tcgcgaaaga aactcgagac
     4741 tccaattaga gcttcatgcg cgagccgttc gacctctggc gaatgttgtt gcatgaattc
     4801 gacgaacgca tcccagtctt gaagccccag ctcctgcatg cgcctttgcg agcgccgcac
     4861 taaagtcgct ggcttgtaga tgctgaaatc gatttgggtg acctgcgaca tgagcccgat
     4921 caaccgttcc agcgcggccg actcgcgaac ttcattgacc cgcgctccga cgtcttcccc
     4981 cactctctcc aaaatcacct gagccatccg ttgtggactc agcacgtggt ccggctttcc
     5041 cccttcgatc gcactcgccg gcatgccatc gaaggccgcg gtgccaggct cctgcaccaa
     5101 caccaatccc ccccggcggt gaatctcctt gcatccacgc gttccgtcgc tgccggttcc
     5161 agagagcacg atccccaccg cgcgctctcc gagagccgcg agcgaacaaa agaagaaatc
     5221 gatcggatga tgcaagcctc cctcggctcg atcttggagg tgcaaccgcc catccacgac
     5281 ggacatatcg cgctgcgcgg gcatgaggta cacggtgttc ggctcgatcc tcatcccgtg
     5341 ctgcaccctc gagatcggca ttgccgtatg ccgcgccaac agctctggca tcaggctctt
     5401 gtaatcggga gacaggtgct gcacgaccac gaacgccatt ccggtaaacg gaggcgtcga
     5461 agcgaagaac tcgctgagcg catcgagccc acctgcagat gcgcccaagc ccaccactgc
     5521 cgatggcgcc aaggagccag ccggcagctt gtcggcactc agctggctca cgcgggagta
     5581 cctctcgagt acaggagcat gatcgtcatg cgtttacgag gcagattgct acagcacttc
     5641 gcgagacgca aggcgtggac ggaaaccgtc agaccactat ccaaagacgc ggccgagcgt
     5701 tgtccaatac tcaagggcgg tctgcaggtc agcgcgtcat ggctttgact cgctcgcgaa
     5761 acccagattc gatatcagcc actgcgtcgg gtatccgcgc catctccgtg cgctgtctcg
     5821 gacgagtggg ctcgggctcc cggtcgagct gcgctggact cgcccaaacg cacgcggaca
     5881 aagcggatcg ctaatcggta gctgctcgcc ctatgccaga gtgacgaaat ccgtcgtcgc
     5941 catcgatacg agcaggaatt tcatgactcg acaggggcaa agcatccgag ccgacatgaa
     6001 cgagcgcgac tgagtgagca cgttgttcag gtcgtgtgcg acggctcggg ccaggcgctg
     6061 cagcgcttgc atcttctcca tgcgcgcttc cgcctggcgc tcgccgccag cggagcgcgc
     6121 agctccgccc gctccgcttg ttcgatcgaa attggtcagc gtgtctgcga ccgcgctcgc
     6181 gaagctagcc tccatctcgg tccagcgccg ggtaccgccg caatgctcgt gacataccac
     6241 cccgatcatg gcgccatccc ggtagatcgg cgcatcgagc agctcgccta cgccggtcgc
     6301 gctcaggtag cccgtcagtt cctgcgtctt cggatcagac gcggcacctc cgcaaacacg
     6361 accctccggc tcaacagcgc cgaaaagtcg cttggacagt ctcggcgctg cagcacccca
     6421 gtcacattcc cctcgcctcc cgagatcgag tactgcgccg tgcacgtcag cgcacgcccc
     6481 tcctccgcaa acatccaaac cgacactcga ggtcgtgttc gaagaaaccc ccttcgcgca
     6541 ttcccgaccg gtttcggcaa gggcactcac atacaacgga ggaaggctcc tcacatgcga
     6601 accagcccgc tgcgcgaatg attcacaggt cacggcacgc cgaactagta tcgacacgca
     6661 tgggtgaagt ttacgagagg ctgttacgcg cgattgctcg gaacgatgac gaaatccgat
     6721 tcgcggtgag gtgtggttcg cgggtgtacg gcacggcaac gcccgcctcg gatgaggact
     6781 ttttcgtcgt actccgagac cgggaggcaa aaagagatct cgtctgggta gatcacatga
     6841 acgtcgtaat ccacggtaca ggcgcgttcg aggccgctct cgccgaacag agcgtctttg
     6901 cactcgagtg tctatgtact cccgccgagc accgtctcaa ggaggcgacc cctcgattcc
     6961 cgctcgaagt gcggcgagcg cggctgttcg actcggtcaa agaccgctct gattccgact
     7021 tcaagaaagc cctgaagcgc tacgatgaag accccgccgc cgcgcgcaag cgagtctttc
     7081 atgccttgcg ggtgctctcg ttcgctcggc aactgctcga ggctgggcga atcactgact
     7141 tctcggcagc caacggctgg tgggaggagc tggccgacgc gaccgagctg acgccgcaag
     7201 acatcgaacg gcgctttggc tcgattcggg aagagctcat cacacaactt ggcgcgctca
     7261 cgcgacgtta gctgcacctt ccggagctct tccctcgaac gttcattctc gcaatcgttg
     7321 cacttggatg acctgatgca tccagtgttc gtcggtggtc acgtgatcgc gacgcaccca
     7381 cttggcgaga cttctcgaga actgcgagtc ctcgaactcc ccggcgcggc gaacgacgac
     7441 accttcacgc attcccccga acgcagacgt ctcgttcgag agcgcctccg tgagctcgtg
     7501 aagcgcagac tcatcggaaa cacggccacg gaaaagaagc ggcaccgtcg ggagcccgag
     7561 ctcgagcgcc tgtgccgcca ccatgtccca atcccaccac atcccccgta cgtcgtcccg
     7621 caccccgaac accagcgagt agtccggaag cgcctcgtac accaaagagt gaacggcata
     7681 acaatactca cagaacaccg acagtccttc ggagatcaaa tgcgcgagcc gcgcatgcgt
     7741 cgccttggcc aggtcgaagc tgcgatgacc gggaggaccg gcgtgcgagc gcgagaacac
     7801 gtgatcgcgt gtatacgtga gattactgcc gtcgcacttt tcggtgatta caatctccac
     7861 atcgatcagc cccgagacgt cgagcaaacg cctatcgtcc gacgtaccac cgggagacca
     7921 tggcaagtga aaggagcgcg gatacttcgc cgacacagcg ggcggaagat agcaggcgca
     7981 agccaacatc gccctcggat tctgctacaa cgccgatcgt gctcgacgaa ttgctgtcgc
     8041 caaccccgcc ggaatatcgg gtcgattggg agtcgctcca ggccttcgaa tgggtgcgtg
     8101 ccctcgaagc ttgcccgcaa gatccgattc accacgccga ggggaacgtc tggattcaca
     8161 cgcgcatggt tttggagacg ttggccgaga tggcggcctg gcgcgcgctc ccgcccgggg
     8221 accagcacgc cgtataccta gcttgcttgc tccacgacgt ggcaaagccg gcaacgactc
     8281 gaatagaaga ggacgggcga gtcaccgcta aagggcactc gcgccgagga gaggtactcg
     8341 ctcggcgcct gctttgggag ctcggggtgc cgttcgccac gcgggagctc gtatgtgggc
     8401 tcattcgata tcaccaactc ccgttctacc tgatcgagcg tccggatgcg caacggctca
     8461 cggcggagat cagcctggtc gcgcgatgca acctattggg catcgttgcc gaagcagaca
     8521 tccgtggccg actgtgcagt gacttgccgc gaatcctcga taacattgcc ttgtatcgcg
     8581 agttctgcgc tgaggagggc tgcctcgcta agccaagagc gttcgcctcg gcgcacacgc
     8641 gcgtgcaata cttccgcgct gacggccgtc atcccgacgt agaagtttgg gacgacacgc
     8701 gctcggacgt ggtcgtgatg tcgggattgc ccggttccgg caaagacacg tacattcgcg
     8761 agaacttccc cgagtggccc atcgtgtcgc tcgacggctt acgcgaagag ctggatgtgg
     8821 accccaccga caaccaaggt ccgatcgtcc agctcgcgcg agagcgcgcc cgcgaacacc
     8881 tacgtcgtgg cgaaagattc gtctggaacg ccaccaacat ttgtaagcgc tttcgtggtc
     8941 ccgtactctc gctcatggcg gactaccacg cccgcatgca cgtcatttac gtcgagtccc
     9001 ccgccgttcg cttgttttca cagaatcgca accgccccgc gatggtcccc gaaaaagcca
     9061 tccgcggcat gctcgagcgc tgggagattc cggatctcac cgaagcgcac gaggtgacgt
     9121 atatagcgga gtgagcgtgt cgacctctac tggtagtgcc agggcgccta caagctcaac
     9181 gacgttcgag caaggcctct agttccgcaa tgcgggccaa cgcggcttcg gcgcgttgcc
     9241 tctcggcgtc ggcgcgcttg cgctgttcgt cggcgcgctg gcgctgctcc tcggcgcgct
     9301 ctgagtcagt cgggaatagg atatcgccat ctgggtcgag cgctaggcgc acgcgttggc
     9361 ggggccgttc ggtgccgacc aagcgcacga agcatcccaa tactcgcgaa cgcacccggt
     9421 ctgcctgcgt gcgttcctgg agctcgagcc ctcgcgaggt gcgttcgaag cgctgcagtc
     9481 ggtagccctc tctgcgtttt aggtaatctg gatctacgac gatgagctcc tcggttccga
     9541 ttcgatggtg cagccgggga gccacctcat agtccttgcg ccaatcctgg gagcagatct
     9601 ccaaagcgaa gctcggatga atgccactct cccagatctt ccaggaaggg atatccaaat
     9661 cctgaggaac gtcgggtaac acgtaaatgt cgggcgcaat gcgctgggag acctgcccct
     9721 tctcgaagta gatgaactga tcggagccga cgaaacacgg agtaccgcgc tcggcgagcc
     9781 agcgttcgat gagaggtcgc agcacttcga gcatccagca ttgcaggatt tcttccccca
     9841 cgggctcctc cacgggatac accgtcgggt catcaggagt tggggctgga gtgcgcatga
     9901 gaccgaacga taccacgcgc gtggcgcccc gccgaccggc agtgtgcatg cagggccatc
     9961 tagcacgagg agacgagaat gcctgaacaa aagtgcgcaa atctccgcag gaatcagggt
    10021 tatcccgcgg gtcgcaacac agtcactcgg gatatcccga gtgccgtcaa ttggccctga
    10081 tgagttcatc gagagctgac gatgcagcga actccagaac ccgctcctcc ggtcggcaac
    10141 ggcgtgactg ccgtgactac ttcctcgcgc cgcccttctc caccacttgc tgaacagcgc
    10201 tcaagccgtg gtcgatcaac gcaatgaacg cggcgttggc ctttttggcg gagccgccgc
    10261 tggagaacac tctctcattt gcctgcggta gccgctcgtc cgtcaagctg caaaaacatt
    10321 acaagggggc cggcgcgacg ggcggagggc tgcccggagc gcgagggtga tgggcggcca
    10381 cgaaatcacg agcgcgaccc ctcgtccgag cgacacccga gcgcagccgc tctgctcacc
    10441 gcccgtccgc ccaccgcgca cgccgcacaa ggtaacgccc gcggcggatt ctgcacgagt
    10501 gcacggcccc acattcgccg accaaagggc tcgtcgcgcg ccggccgccc tagttactcg
    10561 cgtagaacgc caattggggt caggcggaga accgtctgcg ggccatgctc gcactccaaa
    10621 tctcggcgtc gcctaggtca cgttgatgac gcgcttcgat ccgcgcaccg ggtaaatgca
    10681 gtgaccggga gagggccggg agtgggccga atcgactcga atatatgtct cggtttgccg
    10741 ctacaggtgc ttcggtccat gcgtggctct tcattgcgac aatcgaccct gcgtgggcgc
    10801 tcacgaacgc gcagcagccc gcaattcaca aggcatttgt ggtttcctcc gctcgaaccc
    10861 aactcggcat gagggttgca gaaggcgaga cgcgctgctc acaagaggag acgcggaggt
    10921 acagaatcga tgtctattcg aaatctaaaa gcatttgggt tgggctcact gttgttgatg
    10981 gctttcggtt gctctgccga agcgggcgaa aacttcgagt cacaagaatc gcagagcacg
    11041 caacaagcga tcgtggtttc gccgcagaaa ctccacgtag tcggggtgac ctcatcgggt
    11101 ggccttcgcc atgcgctgcg cacggggaca agctggtccg gattcggcga cgtggagggc
    11161 caagcaggcg atattggcac cgtgaccgac gcacaagctg aaatctcgct cggcggactg
    11221 cacgtgatcg ccattgcggg cggagcgttg tttcatacga tccgcagcga aaccagctgg
    11281 cagcccttcg tgaacatggc tgtgccgcta ggcaccgtcg gcacttacaa ttccgtagcg
    11341 ctcgcgaacg gcaccgccgg gacacacgtc tgcgtgacga ccaccgccgg cgggctcttt
    11401 cacaccatcc gcagcagcac cagttggcaa ccctggggtg acgtcaagct cgcgagcaac
    11461 agcaacccgg gaagtctaaa gcgcgtcgct tgctcggtgc acagcgttgt cgtaggtcaa
    11521 gtgcccgctc cgcccggcgg gatctcatta ccaatctttg gagacgagct gcacgtcgtg
    11581 gcgctcacca cctcgggagt tccctaccat gccattcgcc acccgaacgg ttcttgggac
    11641 acgctcaaca acgtcaacgt gccgaccggc aacaccaccc cgttttcgga cattgacatc
    11701 gatgtagacc cgaatggcga actgcacgtg gtcggtacgg ggtcgaacct acaataccac
    11761 agcttccgca cctcgggcgg atggtcggta ttcggcgata tcgagcagcc cgcgggcgat
    11821 ccgggcaatc aacagagtgg agccgccgta gctctagacg aaggcctcta tgttttcgac
    11881 cagctgtcca atggcgggct gatgatgacc cgccgtggtt ccggcgcttg ggacgccttc
    11941 atggatgttc gagtggccac cggaagcact gagtcattta agaaggtatc cacggctggt
    12001 gagtctgccg tgatcggccc gaactgatct aaacgataca cgagttcgca aaaagggggt
    12061 gtggatccta accgagcggg cattcacccc cctccgctcg tgtcgaaacg cgacctagcc
    12121 gcgcgcacga tcacgagcgc ccgtacaacg aagagtcgat gcgggaatgg atttgcggcc
    12181 aggccctcga tcgggcatgg gcatggttga tgcctgagcc ttgcgaaact ccgacccaga
    12241 tagcttttga gctcaaaact ctatcgagtt cgcctgcaca cgactcattt tcgagttgga
    12301 cgacggtcta gccgtgcttc gaaagaagac caggagtcgg cgttggatta ggcaccgggc
    12361 cgtcattgct tgtctgctgg cagccggact gcccatggca tgctccaaat ccccgatgaa
    12421 ccatcagccc accccgggag gcggcgcgcc ggctcatacc gaaagagccg caggttccgt
    12481 cctgagcccg gcgccgctga gttcagtcgc agcgccaact ccgacgcggg ggctcgagtt
    12541 gacggagctc aagggcttgg agctcgatgg gatgcatgtg gatggcgttg cgatcgagct
    12601 ggacttagcc tctgcggtgc tcgagcctcg cgccgtaccc gatcgtcggc tcacctcact
    12661 cgcagcagac ccgacctttt gggtcgcggt gaatgcgggc tacttcgagc gagacgggtc
    12721 gccgtcgggg ctactggcga gctcagcccg ccgctatggt ctgctcgacc gacgtggcgg
    12781 ctcaggcatc gtgatcgtga gcgcggagcg agctcaactc gtgccattcg aaggctttcc
    12841 ttcgaattcg ttccgcggcg aactcgccgt gcaatccggc ccgcgtttga ttgagccgaa
    12901 tggcagcttg ggcatgcgca gcgacgatgg ccagcgagcc cctcggacgg tgctgtgcgt
    12961 catgcaccaa ggcagaagtc tagcgctggt gttgctgtgg tcgcgagaag agccggcccg
    13021 cggccccggg ctcatgacgg tggcccgcct cctgaccggg ccgagcccgc gaggttcaga
    13081 acgttggtgt gaacaggcct tgaacctcga cggcggtcct tccacgggca tcgtctactc
    13141 gcgggcgttt acccggcgag tcactcccga acatctgccc ttgggtccgg taccgtgggc
    13201 gctggtggcg cgaccccgag tcggaaccgc caacccatcg tcggccccgc ctcagccgta
    13261 aacaatccga attgtccatg gatgtccatc gagagcgccc gcggcaagaa tagtcacctg
    13321 attcgtgttc tacatcccgc ccggaagcgg ccagttcctg atcatgcgga gaacttcaac
    13381 accgaaagag tctgc
//
LOCUS       DASTKU010000351         4660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_67644,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000351 DASTKU010000000
VERSION     DASTKU010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67644"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFQ61_12195"
     CDS             <1..382
                     /locus_tag="VFQ61_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein"
                     /protein_id="HET9955262.1"
                     /translation="LFERLPPGAMVLLDELCSGTNPSEGEEIFELVVSMLGRLQPQAF
                     ITTHFLLFAARLERERKISGLAFLQVELDPDRRATYQFVPGVAQTSLAGHAAQRLGVT
                     AEQLASLIERNASKRLPAVYETQV"
     gene            391..1386
                     /locus_tag="VFQ61_12200"
     CDS             391..1386
                     /locus_tag="VFQ61_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955263.1"
                     /translation="MPTFLITRRMSPELAARVLASVRGQRAAPVARRSRPSTMAQLRL
                     AAVLLVAALVTSIVIWRQRSHEHLEAARAELLAAVRRQANSLNPSQKGIAARAELWIA
                     RFTGRYEGDWVDPDLQTEEQLTQRLAQPLLYVKGPIDGFRDSPQIAETARNSFKDAFV
                     ACLVRPPEARSESALLARVRQVGSRGEQSETLGHVQRLHGALAGLPFLGDAWAKHIQT
                     APDTLEIDKLRRLFEKAPIESAKKAAQSSILLLALDEPGELTVPAELDGERPHAVRAA
                     LIDLDQGRWLLRVRRTVDPRKLSSENRATRASALDACGFALEVRQAVSQVRSASR"
     gene            complement(1396..1863)
                     /locus_tag="VFQ61_12205"
     CDS             complement(1396..1863)
                     /locus_tag="VFQ61_12205"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET9955264.1"
                     /translation="MVTPVQAKRLALALPETEEKSHFGQPDFRVRNKIFGGLSRDLVT
                     ASVKLPSARQAELVESQPEVFSAATGAWGRSGWTIVQLDGVEADVLKELFTLSWQAIA
                     PRSLVEAQPSAAVNTHPRRVPTARTKSSPKAQTTPRKQAGKKRPKRVSRVSKE"
     gene            complement(1939..2559)
                     /locus_tag="VFQ61_12210"
     CDS             complement(1939..2559)
                     /locus_tag="VFQ61_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9955265.1"
                     /translation="MSITFYTAPWSSASPVQLALAELGVPHEAISLNLQAKEQKKAEF
                     LKLNPNGKVPTLVVDGTPMFEALAIMQWLGDRYGVEKGLWPEPSSKDRLTALSWSTWA
                     YVTMGSHLYRLVLTSSERADPALHSAKHAEQAKNELSALLRILDERLSAQPYLLGAKY
                     SLADLIVVNVVNFGKTVGMPVDGHANVQAWVARCFERPAYKQLYSP"
     gene            complement(2691..3935)
                     /locus_tag="VFQ61_12215"
     CDS             complement(2691..3935)
                     /locus_tag="VFQ61_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9955266.1"
                     /translation="MSPRSGPGSAATEPLAAAKAKALAEPGGSQHEELQIRRLQQVAR
                     SRPQQAEAWVELGKAWVIEARRSSDPGFYWNAEACAELALAIDGDSRPALNLRGLVLL
                     NGHRFRESEALTRSILDRDSDDAVAWGTLSDARLELGDLEGASEAAERMLSIKPNLPS
                     YSRLSYLRWLRGDEAGALESARLAIDAGGGARGTEALAWVLVQTAQLFWQRGDYPGAR
                     AGFEQALAVMPEYPPACVGLGKLALAEQQATRAVTWLKLAFSHSALAETAWLLSDALR
                     LTGDSAAAADWLSKAEALGQHDPRTLSLLYSVQNRNAARALELAEHELEVRADIYTLD
                     ALAWAQFRLGRYSEARQSIDRARRWGTRDARLLFHQGAIYRAVGELARGREWIATALR
                     LNPYFDVTEAAEARALLGRAPS"
     gene            4326..>4660
                     /locus_tag="VFQ61_12220"
     CDS             4326..>4660
                     /locus_tag="VFQ61_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955267.1"
                     /translation="MTLNSNPRAPAIFRRVWPTLGPVLLLMACGADRNPSGAALPSSE
                     PEPEPQTGPFKSLAVAARDSTTFTEPRAGVPLADGSVAFLAATTTEPERVGLFLQTAE
                     GPRLLYAGD"
BASE COUNT          848 a         1605 c         1404 g          803 t
ORIGIN      
        1 cctcttcgag cgcttgccgc cgggggccat ggtgctgctc gacgagctct gctccggcac
       61 caacccgtcc gaaggggaag agatcttcga actcgtagtc tcgatgctgg gccggctcca
      121 accgcaagcc ttcatcacga ctcattttct gctgtttgcc gcgcgcctcg aacgggagcg
      181 aaagatctcg ggcttggcct tcttacaagt cgagctcgac cccgatcgtc gtgccaccta
      241 tcaattcgtg ccaggggttg cgcagacctc actggcggga cacgccgccc aacggctcgg
      301 ggtgacggca gagcaactcg cgagtttgat tgaacgcaac gcctcgaagc gcctcccggc
      361 cgtctacgaa actcaggtgt gaggacgccc atgccaacgt tcctgatcac gcggcgcatg
      421 tctcccgagc tcgccgcgcg ggttctcgcc agtgtgcgcg ggcagcgcgc ggcccccgtg
      481 gcccggcgtt ctcgtccaag caccatggcc caacttcgtt tggcagccgt gctgctcgtc
      541 gcggccttgg ttacgagcat cgtgatctgg cgccagcgca gtcacgaaca cctggaagcg
      601 gcgcgagccg agctgctcgc ggcagttcgg cggcaagcga atagcctgaa tccgagccag
      661 aagggcatcg cagctcgcgc tgagctctgg atcgctcgct ttaccgggcg ctacgagggc
      721 gattgggtgg accccgatct gcagaccgaa gagcagctca cgcagcggct cgcgcaaccc
      781 ctgctttacg tgaagggtcc catcgacggc ttccgagatt caccgcaaat cgcggagacc
      841 gcgcgcaact cgttcaaaga cgcattcgtt gcctgcctgg tgcggccgcc ggaggcgcgc
      901 agcgaaagcg cgctactagc ccgcgtgcgc caggtcggct cgcgtggcga gcagagcgaa
      961 accctgggtc acgtgcagcg cctccatggc gcgctcgccg gcctaccctt cttgggagac
     1021 gcttgggcca agcacatcca aacggcgccc gatacactcg agatcgacaa actgcgccgc
     1081 ttgttcgaga aagcacccat cgaatcggcc aagaaagcag cccagtcgag tatcttgctc
     1141 cttgcactcg acgagccggg agagctgacg gtgcccgctg aactagacgg cgagcgtccc
     1201 cacgccgtcc gagccgcact catcgacttg gatcaagggc gctggttatt gcgcgtgcga
     1261 cgcacggtcg atccgcgaaa gctctcgagc gagaaccgcg ccactcgggc ttcggcgctc
     1321 gacgcctgcg gttttgcgct cgaggtgcgg caagccgttt ctcaggtgcg aagcgcttca
     1381 cggtgaagcg ccgttttact ccttcgagac cctcgaaacg cgcttcggcc gcttctttcc
     1441 ggcttgcttc ctgggggtgg tctgcgcctt cgggctggat ttggttcgag ccgtgggcac
     1501 acgtcgtgga tgtgtgttta ccgcggcgct cggctgcgcc tcgaccaagc tgcgcggcgc
     1561 aatggcttgc caggaaagcg tgaacagctc cttcagcacg tccgcttcga ccccatcgag
     1621 ctgcacgatc gtccaaccgc tgcgccccca agcgccagtc gccgcgctga acacctcagg
     1681 ttggctctcg accaactccg cctgacgcgc gctcggcaac ttgaccgaag cggtcacaag
     1741 atctcggctc agcccgccga agatcttgtt tcgcactcga aagtcgggct gcccaaagtg
     1801 cgatttctct tccgtctccg gaagggccaa ggccaaacgc ttggcctgca cgggagtcac
     1861 catgatccta ggatagacga tcggcgggcg ctcgacagtc ggtcgcgtca cccgcctcag
     1921 gtccttcaag cagagcgctc agggtgagta cagctgcttg taagccggtc gctcgaaaca
     1981 gcgggcaacc caggcctgca cgttggcgtg cccgtcgacc ggcatgccca ccgttttgcc
     2041 aaaattcacg acgttgacca cgatcaaatc cgcgagcgaa tacttcgcgc cgagcaagta
     2101 cggctgcgcg gagagtcgct cgtccagaat tctgagcaac gcggagagct catttttggc
     2161 ctgctccgcg tgcttcgcgc tgtgaagcgc gggatctgcc cgctcgctgg acgtcagcac
     2221 cagccgataa aggtgcgacc ccatcgtcac gtacgcccag gtcgaccacg acagcgcggt
     2281 caagcgatcc ttggagctcg gctcgggcca aagccccttc tcgacaccat agcggtcccc
     2341 gagccactgc atgatggcga gcgcctcgaa catcggggtg ccgtccacca ccaaggtcgg
     2401 caccttgcca ttaggattga gctttagaaa ttcagccttc ttttgctcct tggcttgaag
     2461 attcaaagaa atagcctcgt gcggaacacc cagctcggcg agcgcgagct gcacggggga
     2521 ggcactggac cagggggcgg tatagaaggt aatcgacatg gggatgcggc tcctgctttg
     2581 gttggctcgg tgcgtccgag cagcacccgg aagctactcg ggattgcgga cagaatcagt
     2641 ccgcaatgcg agcctgccgt ttttgtcggc tccagtggcg attctcgccc tcaggaaggg
     2701 gctcggccca acaaagctcg cgcctctgcc gcctctgtca cgtcgaagta gggattgagt
     2761 cgcaacgccg tcgcgatcca ctcccgtccc cgcgccagct cgccgacagc tctgtagatg
     2821 gctccttgat gaaacagcag ccgcgcgtcg cgggttcccc agcggcgcgc ccggtcgatg
     2881 ctttggcgcg cctcggagta acgccctaac cgaaactgcg cccaggccag agcgtccaac
     2941 gtatagatgt cggcgcgcac ctcgagctcg tgctcggcaa gctccagcgc acgcgcggcg
     3001 ttacgatttt gcaccgaata caagagagaa agagtgcgtg gatcgtgctg cccgagcgct
     3061 tccgccttcg agagccagtc cgcggccgcc gcgctatctc cggtcaagcg caatgcatcg
     3121 ctcagcagcc acgccgtctc ggccaacgcg ctgtgcgaaa aggccagctt caaccaggtc
     3181 acggcacggg tcgcttgctg ctctgccaac gccaatttgc caagccctac gcacgccgga
     3241 ggatactccg gcatcaccgc gagtgcctgc tcgaacccag cgcgggcacc cggatagtcc
     3301 ccccgttgcc agaacaactg cgccgtctgc accaagaccc aggccaacgc ctccgtgccg
     3361 cgcgcgccgc cgccggcgtc gatcgccaga cgtgccgatt cgagggcgcc cgcttcgtca
     3421 ccgcgcagcc accgcaaata cgagagtcga ctatacgagg gcaaattggg cttgatcgag
     3481 agcatgcgct ccgcggcctc actagccccc tcgagatccc cgagctcgag gcgtgcgtca
     3541 ctcagcgttc cccaggccac ggcatcgtcg gagtcacgat cgaggatcga ccgcgtcaat
     3601 gcttcggact cccggaaacg atgtccattc agcaacacca gcccacgcaa attcaaagcc
     3661 ggtcggctgt cgccgtcgat ggccaacgcc aactcggcac aggcctccgc gttccaatag
     3721 aagccagggt cgctcgagcg ccgcgcttcg atcacccaag ccttacccag ttcgacccaa
     3781 gcttccgctt gctgcggtcg tgaacgggcg acctgttgca gtcgtctaat ctgcagctcc
     3841 tcgtgttggg agccgccggg ttcggccagc gccttggctt tggcggccgc cagcggctcc
     3901 gtagcggctg aacccggtcc cgagcgcgga gacatcgagc gactgtgctc gccgtttaca
     3961 ctccccggcc ttgggttttc ggccccatcc gcaaacatgt gggaagagct ccagcccgca
     4021 gcgagcgcca agcacgaagc agccaaacca gccagccagt gacgagataa ccgcatgcac
     4081 cgacggttac gaccgtgcat gcctggcgga tcatcgcgcc ccccgcgagt ggcgacgatt
     4141 tactcaatca tcgtttcgat acacgcgtgg caaatccgaa ttgggagttg gcccgtagcg
     4201 agaacacggt accgccacgc gaagccaaac cgaggcccgc ctggcagcgc cgaaaaacac
     4261 ggcccgcctg tgcgggtcga aaacacgggg cccgcctggc aggccccatc gcaacaggag
     4321 cgcgtatgac cctgaattcg aaccctcgag cgccggcgat attccggcgc gtgtggccga
     4381 cgctcggccc cgtgctgctg ctcatggcct gcggcgcgga ccgcaacccg agtggcgccg
     4441 cgttgccttc gagtgagccc gagccagaac cgcaaaccgg cccgttcaag tcactggccg
     4501 tcgccgcgcg ggattccacc acgttcaccg aacctcgcgc tggcgttccc ttggcggacg
     4561 gcagcgttgc gttcctcgcc gcgaccacga ccgaacccga gcgcgtcggc ctcttcttac
     4621 aaaccgctga agggccccgt ttgctctacg ccggtgatca
//
LOCUS       DASTKU010000352         4701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1852661, whole genome shotgun sequence.
ACCESSION   DASTKU010000352 DASTKU010000000
VERSION     DASTKU010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852661"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFQ61_12225"
     CDS             <1..408
                     /locus_tag="VFQ61_12225"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HET9955268.1"
                     /translation="MTDGQALDVGIQLELGHARSAHRAAQLLRDAGLYNDALNRLYYA
                     LYHVVTALLLSSGVEPRRHRALPGLLGTHFAHALGPAELAAVARAATWRDLADYERTW
                     QANAEVTAQAFAEIEPLMARLHELLDAGASTPR"
     gene            complement(566..1540)
                     /locus_tag="VFQ61_12230"
     CDS             complement(566..1540)
                     /locus_tag="VFQ61_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9955269.1"
                     /translation="MERLDLNLLLALDVLLAESSVLGAARRMHLSASAMSRTLTRLRA
                     ATGDPLLVRAGRGLVATPHALALRDRVDALVKEASAVLRPAGHDVDPASLDATFTIRA
                     GRWFMESIGSSLVSATLAAAPQVRLRFLPKHDKDLRPLRDGTIDLEIGLVGELAPEVR
                     TQFLVRDDLVGVARVGHPIFSMRKITAKRFASLQHVMASQWGDFTGPVDESLAKLDLY
                     RIVAVVVPGFPDALRIARVTDLVTVAPRSMLEGPAPVEHETRAGLRAFALPVEVPEIR
                     VSAMWHPRLDADPAHRWLRRAVSRCFKDTLSKSGRTALPPANHDVRRT"
     gene            1838..2905
                     /locus_tag="VFQ61_12235"
     CDS             1838..2905
                     /locus_tag="VFQ61_12235"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HET9955270.1"
                     /translation="MQPAEAPGCSQGTPYSFWVKDGDPSKVVVIFSGGGACWSAETCA
                     LSETPSYRPFAGPDQNPSEWGGVFDTDNPENPLADYSFVFLPTCNGDVFLGDSITTYA
                     TPTSGPPADTFQILHEGYDNAMSALTWMFGTYRNPETVAVLGWSAGAIASPVYTHIVA
                     QHYPDAAVSHFGDGAGAYRVGERLEPLFESWGIANALKNIVGFEDMRVDGLSLEDLYI
                     RAAELHPDIIFHQYNERHDAIQALFMRLMGVDSPDVPANLDDAHAYIRAHVSNFRTYT
                     SWGHDEGIIGGYYDGVLAKNALENRGRPYVLDRLYTYQTNGVRFLDWFTAAVRGQPVE
                     DVTCEDCETPEYHWQRPRSAE"
     gene            complement(3482..3781)
                     /locus_tag="VFQ61_12240"
     CDS             complement(3482..3781)
                     /locus_tag="VFQ61_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955271.1"
                     /translation="MRDSKAWTSAAIELGLEVPLAGGAALGDLQAHPWLPQICLRQPH
                     EGGAEFNQPAHHPRGVLVARLDPHVQILSCSWPAVYANRVGTHDEEAGISGRECA"
     gene            complement(3880..4197)
                     /gene="tnpB"
                     /locus_tag="VFQ61_12245"
     CDS             complement(3880..4197)
                     /gene="tnpB"
                     /locus_tag="VFQ61_12245"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET9955272.1"
                     /translation="MIEAFIPTSATRVVVYLQPVNVRWGVDKLRAFCRDEMRMEPDDS
                     TCFLFVNRARDTLLLYHRGYTGEQTLIKKLDKGSFVLPAPEENGRPFAVLRPAMLGRL
                     FRS"
BASE COUNT          884 a         1440 c         1527 g          850 t
ORIGIN      
        1 atgacggacg gccaagctct cgatgttgga attcagctgg agctcgggca tgcgcgctct
       61 gcccatcgcg cggctcagct gctgcgcgac gccggcctct acaacgacgc gctcaaccgt
      121 ctttactacg cgctctacca cgtggtcacc gcgctccttt tgagctcggg cgttgaaccg
      181 cgacgacatc gagccctgcc gggtctgcta ggtacccact tcgcccatgc actcggcccc
      241 gccgagcttg ccgccgtcgc gcgagcggcc acgtggcgcg acttggccga ctacgagcgt
      301 acctggcaag ccaacgcaga agtgacggcg caagcgttcg cagagattga gcctttgatg
      361 gcgaggttgc acgagctgct cgacgccggt gcttcgacgc cccggtaacc ggttcgctgc
      421 ggcgagccat cgggtcccag tcagcgttgc cggagttggc gttggcttca atgtggccac
      481 cgctactcag cggtggagtc acccgcaaag cacccgttca gtgttccgcg atcatcgttc
      541 accctccaga ctcgcagtgt gccgctcacg tgcgtcgaac atcatggttt gcagggggga
      601 gcgccgtgcg gccagacttc gacagcgtat ccttgaagca cctcgagacg gcgcggcgaa
      661 gccaacggtg ggctggatcg gcgtcgagcc gtggatgcca catggccgag acacgaatct
      721 cagggacctc caccgggaga gcgaaagccc tgagccccgc tcttgtctcg tgctcgacgg
      781 gcgccgggcc ctcgagcatc gaccggggcg cgaccgtgac cagatcggtg acacgagcga
      841 tgcggagcgc gtcggggaac cccggaacga cgaccgcgac gattcggtaa agatcgagtt
      901 tcgcgaggct ctcgtctacg ggaccggtga agtcacccca ctgggacgcc atcacgtgct
      961 gtaatgacgc aaatcgtttt gcggtgatct tacgcatgct gaagatggga tgacctacgc
     1021 gagcaactcc cacgagatcg tcccggacca ggaactgcgt tcgaacttct ggagcgagct
     1081 cgccgaccag tccgatctcg agatctatgg ttccgtcgcg caggggtcgc agatctttgt
     1141 cgtgcttagg caagaagcgg agtcgaacct gcggcgcggc ggccagggtg gcggagacta
     1201 acgagctccc gatcgactcc atgaaccagc gcccggcgcg gatggtgaac gtggcatcga
     1261 gcgaggcggg atcgacgtcg tgtcccgctg gtcggaggac cgcgctcgct tccttgacga
     1321 gagcgtccac ccgatcgcgt agggccaacg cgtgaggcgt agcaacgagg ccgcgtccgg
     1381 cgcgtacgag cagcgggtca cccgtcgccg cccgaaggcg cgtgagtgtg cggctcattg
     1441 ccgaggcgct caggtgcatg cggcgggccg cgccgagcac gctgctctcg gcgagcaaca
     1501 cgtccagcgc gaggagtagg ttcagatcca gacgttccat gctgcatgga tatctcgagc
     1561 ggctggcggc ggcatagcgc tggacgcaac aataccgtgc acaccgcgcg tctggcgccc
     1621 gtcgacgatc ggacgaagat cccacgacat gtgtcgggcc gaaacgtggc ctcttccgat
     1681 ggccagcaac cagagatcgc cgataggcgc ttcattgaag gatctagtga tgcgaaccca
     1741 actcgttcaa tcttccagac gcgggatcgt ggctggcctc tcgatactcg ccgcgatcgg
     1801 gggcggcaca cctcggcccg ttgctgactg gcggaatgtg caacccgcag aagcgccagg
     1861 ctgttcacag ggcactccct actctttctg ggtcaaggac ggggacccct cgaaagtggt
     1921 tgtcatcttc tctggcggtg gcgcgtgctg gtcggccgaa acctgcgcat tgagtgagac
     1981 accctcttat cgcccctttg ctggccctga tcagaatccc agcgaatggg gcggggtctt
     2041 cgacacggac aacccggaaa acccgctcgc agactacagc ttcgttttcc ttcccacttg
     2101 caacggagat gtctttctcg gcgactcgat cacgacgtac gccacgccaa cgagcggccc
     2161 gcctgcggat acgttccaga tcctgcacga gggctacgac aacgcgatgt ctgccctgac
     2221 ctggatgttc gggacttacc gcaatcccga gaccgttgcc gtgctgggat ggagcgcggg
     2281 cgcgatcgcg tcgccggttt acacgcatat cgtggctcaa cactatccgg acgcagcggt
     2341 cagccacttt ggcgacggcg cgggtgccta tcgtgtgggc gagaggctcg aacctctctt
     2401 cgaatcctgg ggcatagcga acgcattgaa gaacatcgtc gggttcgaag acatgcgcgt
     2461 ggacggcttg tcactcgaag atctctacat tcgggcagcc gagcttcatc ctgatatcat
     2521 attccaccag tataacgaaa ggcacgatgc tattcaggca ctcttcatgc ggctgatggg
     2581 tgtggatagc cccgacgtcc cggcgaatct ggacgacgcg catgcctaca tccgcgctca
     2641 cgtttcgaac tttcgcacgt ataccagctg ggggcacgac gagggcatca tcggcgggta
     2701 ctacgacggc gtgcttgcca aaaacgcact cgaaaaccgc gggcgcccct atgtactgga
     2761 taggctatac acctatcaga caaacggcgt gcggttcctt gattggttca cggcggccgt
     2821 tcggggacag cccgttgagg acgtgacctg cgaggactgc gaaacgcccg aataccattg
     2881 gcagcgcccg cggtctgctg aatgacagcc cgtccgcgat cgcgcgcgac tgttcgcggc
     2941 ggtgctcttg gcgatggagt gcccgttccc agagcaccag atgagttata cggccggcga
     3001 gcgacggacg gcgctactta gtcctgatcg cgggcggcca cgagatcatc ggggcgagtc
     3061 agatgtctac gacggcggta gaacgagggg gggggcgacg gggagaggag ggagacggtt
     3121 caaatcgcgg cggcgcactg cggggctcta tcccgcgatt tcgttggagt ttgagcggcg
     3181 gcttggtgtt tacgctttgc ggcttcgggt tacggttggc tcgtgggaac cgcggggttc
     3241 cgtgatatcc tcgtacgagt gagcacctcg tccgaagcag aagccaggaa agcagcccga
     3301 gctcgcgcag aggtccgcat cttccgggag accgagagcc aggcggaagc cgatgccgac
     3361 gcgctctact gggatcgaat cccgattgat gagcgtgctg agttcgtttg gcgactcagc
     3421 ctggaattgc acgagctttc gaatccgacg cagcgctatg agccccgact ttctcgatct
     3481 gttacgcgca ttctcggccg ctaatgcccg cttcctcgtc gtgggtgcct acgcggttgg
     3541 cgtacacggc aggccacgag caactaagga tctggacgtg tgggtcgagg cgagcgacga
     3601 gaactccccg agggtgatgc gcgggctgat tgaattcggc gcccccctca tggggctgac
     3661 ggaggcagat ctgaggcagc caggggtggg cttgcagatc accgagggcg gctccgcccg
     3721 cgagcgggac ctcgagacca agctcgatgg cggccgaggt ccaagccttg gagtcgcgca
     3781 aggggccgcc gcgcgacggg cgcgcatgac tgccgtatcg cagtacacga gagcgatgcc
     3841 ggcgtgcgcg ataagcccga gcctcgaact cggccgacgt cacgaacgga acaagcgccc
     3901 cagcatcgct ggccgtagaa ctgcgaatgg gcgaccgttc tcttcgggag cgggaagcac
     3961 gaaggaaccc ttgtccaact tctttatcag tgtctgctca ccagtatacc cccgatggta
     4021 cagcagcaac gtgtcgcgcg cgcggttcac gaataggaag caggtgctat cgtccggctc
     4081 catgcgcatc tcgtcgcggc agaatgcccg gagcttgtcc acgccccagc gcacgttgac
     4141 gggctggagg tagacgacga ctcgggtggc agacgtgggg atgaacgctt cgatcatgag
     4201 cctccccata caccagcgac gttaagcaga gcggacagga tccgtcctag caaacgagac
     4261 tcccaccctc gcgagcacca ccggccttcg atgcaagccg cattggcgag gccccagtgc
     4321 gcggccattg tcgagcgaca atcgctggaa gagtagggct cgcgccgcgg aggaagcaag
     4381 cagcaagatt ggcgggttct cctcgtccaa gccgcggact ctggcgtgaa tggagggaaa
     4441 gccctgcgtg cgagcgtggc gcttgccgag gtcctaacac aaggcgttcc tgtctgctaa
     4501 gctgagtcca gcggatttct gaatcgtagg gagagctcgc atcgtcagtg aagccgccgt
     4561 caaagtcacc acgaacccga agacgcatgc tgttctttct cattgccgtc gccttcgcgc
     4621 tggtcttgtt tgcgggctgg tggatgtatc gttggcagta tcgaacggca gatcgagttc
     4681 tgcaggattg ggctattcag c
//
LOCUS       DASTKU010000353         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1406685, whole genome shotgun sequence.
ACCESSION   DASTKU010000353 DASTKU010000000
VERSION     DASTKU010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406685"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(694..1470)
                     /locus_tag="VFQ61_12250"
     CDS             complement(694..1470)
                     /locus_tag="VFQ61_12250"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HET9955273.1"
                     /translation="MSLLQRETVLSVHHWNDTLFSFKTTRARGLRFKNGHFLMLGLEV
                     EGRPLLRAYSLASANYEEHLEFFSIKVQNGPLTSRLQHLKPGDSVMVSQKPTGTLVLS
                     HLLPGRRLYLLSTGTGLAPFMSLIRDPEVYENFEQVVLVHGVRFKRDLAYSGYIENEL
                     PQHEYLGEEVRRKLLYYPTVTRESFRNQGRITDLLRSGKLAADLGLPSLDAERDRVMI
                     CGNPAMLGELVTDLETRGFQEGSSHERGHYTFERAFVARD"
     gene            1544..2449
                     /locus_tag="VFQ61_12255"
     CDS             1544..2449
                     /locus_tag="VFQ61_12255"
                     /inference="COORDINATES: protein motif:HMM:NF008095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9955274.1"
                     /translation="MRYTLRQLEVFLAVARHESVSRAAKDLAMSQSAVSGSLNDLEQA
                     FDVRLFERVGKRLRLNDLGRAFRPHAEALWDQARELENVIENRAGATPLRVGATLSIG
                     NYLMAPLLARFRREEPAIRVGLEVFNTEEIVRRVHNFEIDIGLVEGEVQGQDLRVTRW
                     CDDELYVFCAPWHPLAKKRALDEEDLKKAAWIVRERGSGTRQAFEHAMHGLLPELDIS
                     LELQHTEAIKSAVKAGLGVGCVSRVALVDELEFGSLVRCRVPGRDFRRAFYFVLHQDK
                     FQGPALKSWLNFCVRSVKGAPKARA"
     gene            complement(2530..>2710)
                     /locus_tag="VFQ61_12260"
     CDS             complement(2530..>2710)
                     /locus_tag="VFQ61_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955275.1"
                     /translation="MPRRLNGREISACALERSHSSPFRAEIPRSGHLVSALAMDGSAA
                     PTGNSGRRPWVPRAR"
BASE COUNT          499 a          864 c          842 g          505 t
ORIGIN      
        1 gggcgccgag cggacacgtg agggacaact ctggatgcta ggcggacgcg agtggtgccg
       61 atggcagtga tgtcgagaca gcagcttggg cgcggcagaa ccgcactccc tgtgtatcgg
      121 ggacactttt ttgcgtcaag ccgcgcgctt tccttgtgag ttcattcggc ggaactcctc
      181 ctatgcgcgc tgcactgatc ccagcccaat ccgagggccg tgctgtcccg cggctgcacg
      241 cgacggaacc cgatgtgaac attgttgtgg gagaaacgtc tgcctgacga atctcgaggc
      301 ggctcgaaaa ccgatggtcc gcgcgtaacc aacgtcgcgt cgcgagctgc cgtatcgcag
      361 gcggcggtgt tccaagctgc gacaaccctc ggagacgcac aacaaattac caagacccga
      421 tgactaaacc tccagggtta gcctcggcgg ctcgtctcgt ggttctgtat ccacgcgagc
      481 tttccaacgc caggtacggc gtaaccgtcg tcgtcttgaa acctccgggg cccgcgtgag
      541 tactcgcagt gccgctagag atccagagct gggacccgac agttgaaccg ctggcctctg
      601 gacgacgcga cctcgatcgg ctgacacgac gtggcttgct ggtttcgccg tgtcagccgg
      661 acgcgtggtc gcgcctccgg ggttctatcg acttcaatcg cgggctacga acgcgcgctc
      721 gaacgtgtag tgcccgcgct cgtgactcga accttcctgg aagccgcgcg tctccagatc
      781 ggtcacgagc tccccgagca tggctgggtt accgcagatc atgacgcgat ctcgttctgc
      841 atccaggctc ggcaacccaa ggtccgcggc caacttgccg ctgcgcagca ggtccgtaat
      901 ccgcccttgg ttgcggaacg actcacgggt gaccgtgggg tagtagagca gcttgcgccg
      961 cacctcttcg ccgagatact cgtgctgcgg cagctcgttc tcgatgtagc cagagtaagc
     1021 cagatcacgc ttgaagcgca caccgtgtac gagcaccacc tgctcgaagt tctcgtacac
     1081 ctcggggtcg cggatcaagc tcatgaacgg cgcgagtccc gtgcccgtgc tgagcaggta
     1141 caagcgcctg ccgggcagta ggtggctgag cacgagtgtg ccggttggct tctggctgac
     1201 catcaccgaa tcacccggct tcaggtgttg cagacgcgac gtgagcggtc cgttctggac
     1261 cttgatcgag aaaaactcga ggtgctcttc gtaattcgcg ctggccaagc tgtaagcgcg
     1321 caggagcggt cgcccttcca cttcgaggcc gagcatcaag aagtgcccgt tcttgaagcg
     1381 aaggccgcgc gcgcgcgtgg tcttgaagct gaacagggtg tcgttccagt ggtgcacgct
     1441 cagcaccgtc tctcgttgca gcaagctcat gcccctggtc atagagcagc ttgacccttc
     1501 ggtaaaacgg gttattccgt aaaccaactt cggaaacgcc gatatgcgct acaccctgag
     1561 acaactcgag gttttcttgg ccgtcgcccg gcacgaaagc gtgagtcgtg ctgcgaaaga
     1621 cctggccatg tcccaatccg ccgtcagcgg atcgctgaat gacctcgagc aagccttcga
     1681 tgttcgcttg ttcgagaggg tgggcaaacg tctgcggctc aacgacttgg ggcgcgcctt
     1741 tcggccccat gccgaggcac tctgggacca ggcgcgagaa ctggagaacg tgatcgagaa
     1801 ccgagcggga gcaaccccgc ttcgcgtcgg cgccaccctc agcatcggca actatctgat
     1861 ggccccctta ctcgctcgct tccggcgcga ggagcccgcc atccgcgtgg gcttggaggt
     1921 gttcaacacc gaagagatcg tgcgccgcgt gcacaacttc gaaatagaca tcggcctcgt
     1981 cgaaggcgaa gtccaaggcc aagacctgcg tgtcacccgc tggtgtgatg acgagctcta
     2041 cgtgttctgc gcaccgtggc atccactagc caaaaagcgc gcgctcgacg aagaggatct
     2101 caagaaggcc gcgtggatcg tgcgcgagcg cggctccgga acgcgccaag ccttcgagca
     2161 cgccatgcat ggcctattgc ccgagctcga catctccctc gagctgcagc acaccgaggc
     2221 catcaagagc gcggtcaagg cgggcctcgg cgtcggctgc gtctcccgcg tcgcgttggt
     2281 ggacgagctc gagttcggga gcctcgtgcg ttgtcgcgtg cccgggcgcg acttccgacg
     2341 ggccttctac ttcgtgcttc atcaagacaa atttcagggc ccggccctga agagctggct
     2401 gaatttctgc gtgcgaagcg tgaaaggcgc gcccaaggct cgcgcttgat ctgccgcctc
     2461 gaaataacga gcgcgagtct ccgtcgcaag cctcgtgagc gcagatgatc ggcatgagcg
     2521 ttcccgagct caccgcgccc gcggcaccca tggcctccgc cccgagttcc cggtcggcgc
     2581 tgcgctccca tccatcgcga gcgccgatac gaggtgcccc gagcgcggta tctcggctcg
     2641 aaatggtgaa gagtggctgc gctcgagcgc gcaagcacta atttcgcgcc cgttcagtcg
     2701 acggggcatc
//
LOCUS       DASTKU010000354         7550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1852937, whole genome shotgun sequence.
ACCESSION   DASTKU010000354 DASTKU010000000
VERSION     DASTKU010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1852937"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VFQ61_12265"
     CDS             <1..752
                     /locus_tag="VFQ61_12265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9955276.1"
                     /translation="LTRDGELSEFQPLTAPWRISSIPRTTRPTPEARHQDTLPTGNSR
                     QHRSHRLFDSGVVKMTGVLADAGCYDALRKMGMNTFLEAHAALIVRLARRVARPPTHP
                     EDIAQEAIASLLRWHREKGFDPEKIGNAEAYLRVVVRNTAAQAARRFRGEVPAPDRGS
                     HATQADETRPPDYSIRFADEALHARALLEQLKQRLRPREAVVLALLIEDEMSPDQVAQ
                     SLGMTMNNLYQIRHRIRAAARELLDASAQGV"
     gene            766..1503
                     /locus_tag="VFQ61_12270"
     CDS             766..1503
                     /locus_tag="VFQ61_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955277.1"
                     /translation="MSASEDRDFARLSELAGQAAPSVSGGEHLSASELQRVRTGSEEE
                     DLEHSYRHLATCSSCRARLVERPVLPGKVPSRLRNGAVAAAVVAAACFAGLYMASTRP
                     TLPEQLAIQQLSRSTLMGSDVAPAAEGGNLELSFVAPSSLCAFLLPVGSDGTLLAPLH
                     WFEHEGAKQTLQLAPRNFRAHQGSAEALIVFGSEAATAAVASAWQNHTPTAGNLTALQ
                     NQLAIWAAPQGARVQRIALSQPARDHD"
     gene            2835..3902
                     /locus_tag="VFQ61_12275"
     CDS             2835..3902
                     /locus_tag="VFQ61_12275"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET9955278.1"
                     /translation="MSPSVSVFRRVALLRRRRRASDPLVGLLFVYLSSSACSLQAPKE
                     DEVFGSQGGSSSLQMSGGTGGSGEPSSSSGRQGSSGQGAGASTGGSGGTRSGSDEGGS
                     PITPGGVTGASGGSTSGTSGETGSGGAPAPDIERGLVAHYAFEETSGDSVVNTVSASA
                     SGKCFGTCLHLSGTMGGAIGLRNSEENLNWVELPSGLLSGMSATTLSLWVRDLSASRK
                     DAPAFHFSRGTNEVFFFVPDDVVPDTSTSSGSLGVKHGGAQIISIRGTRSLTDRSWHH
                     VAATWTAAAVSLYIDGMRIGTQSSPGAVPDDLGPTSPNYLGRALSDTIPAINADLDEL
                     RIYDRALEAEELNLLYRQPSK"
     gene            4109..6151
                     /locus_tag="VFQ61_12280"
     CDS             4109..6151
                     /locus_tag="VFQ61_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955279.1"
                     /translation="MNRTAAAIALLLAAGACSREPSESAAGNKPEAQPATGGMAGASD
                     RGGTAGGSTEDSGGAPLGGGTSQPTSSGGANNAGAASIVSGGVTGRAGTSSVEQQGGT
                     SAGSSSSGSAGSNAGAPAVTRPFVCGPSKSPMFQSQEPLTMRIAANFSKLNSAATAEE
                     SGSSGTIELKGAEGVTQLSAKVVARGNSRFRYCQVRPFSLKFTEKQKGNLFEHLGKSV
                     KFVSHCVAKPDAPVNAQAPSLAAYEQRVLMEHTVYQTLDQLSVPSLKTRLVNLTYHDT
                     QTDEEETHPAFVLEPEDEMAERCGMVKVEDLAPGQGDNLKLNQYGVLLLHLLNNFILQ
                     YDWDFQRNVIPLAALDRGEKFNGVYDFDLVAVIRPDYSKNLGRTLAENAAGFADWLRR
                     NQSPALFNEVGSMLQRSDAMLATLQLPGLTEQNRALFTDWLRQFLPALEQFKACEGHG
                     NDATRPACYVPDDHGASPDTASTQQPGEWTTRIEPPGDTDMFSLQLEANQLYSLGASM
                     SLALRDDNGVLIAQSETIEPTSFRVPRSGKFYLEASMPGSSVVSPFHADPQKIDQSVY
                     LYADDAGTSLDTAVPLSMGQAFDGKWELSTGWDQDWFLVHAEPGSSLHVTLNEPHNGT
                     PMLVAQRQDGSDAEQELSLEYGDNDASSLLPEAGDYVLKLSHYGFDQPGTYRIEFK"
     gene            complement(6275..7048)
                     /locus_tag="VFQ61_12285"
     CDS             complement(6275..7048)
                     /locus_tag="VFQ61_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955280.1"
                     /translation="MTLRIIVNLGSGSDPRTLLVPRFFGPDLEGDDYVIFNMDSGHML
                     VSTVLQKRTLESLCGFVRLFVPIDKQDAIPKLLEKPTPSAHLVNLLFTHQPNLVPIIE
                     AMTLLERLALQFIEERLRSTPSRSATYKIQGTVIHVPDPKAGARELALPNFERSKADD
                     VQLPLGSVYANVAISPFGFHPITQATVELLRPKGYFVVIGNQPNKFFKFPAGDRKPGE
                     FGLEPCALPRCLDALNAAPSVHTNGGQVDKLLVSCFQLQ"
     gene            complement(7059..>7550)
                     /locus_tag="VFQ61_12290"
     CDS             complement(7059..>7550)
                     /locus_tag="VFQ61_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955281.1"
                     /translation="SQLKEAKELMLELIKHGTLPVGWELLQAGYGRTRADNPDSAGNL
                     NFKLSKFSNGRPATYYDTTAGENTSVLVLESSAAWTTLPGDSGGPLFAVNRVKGNTHV
                     IGVTLGSDVFATLQAEQQGLAANDTDGAHNNAATALDRVYDGWDELGSMVGLPLVRDH
                     FSL"
BASE COUNT         1509 a         2285 c         2353 g         1403 t
ORIGIN      
        1 ttcttacaag agacggggaa ctttccgagt ttcagccgct tactgcgccg tggcggattt
       61 cgtccattcc ccgtaccact agacccacgc cagaggcgcg gcaccaagac acactaccga
      121 cgggtaacag ccggcagcac cgctcccatc gtcttttcga ctctggcgtc gtcaaaatga
      181 ccggcgtact cgcggacgcg ggatgttacg atgcgcttcg caagatgggc atgaatacgt
      241 ttttggaggc gcacgccgca ttgatcgtgc gcctggcgcg gagagtggcg agaccgccca
      301 cgcatccgga agacattgct caggaggcaa ttgcgtctct cctgcgctgg caccgagaga
      361 agggcttcga tccggagaaa atcggtaacg ccgaggccta cttgcgcgtt gtggtgcgca
      421 acacggcggc ccaagccgcg cgccgctttc gtggtgaagt gccggctccg gaccgcggga
      481 gtcacgccac tcaagcggac gaaactcgac cgcccgatta ctccataagg tttgcagatg
      541 aggcactgca tgcgcgggcc ctactcgagc aattgaagca gcgcctccgc ccccgcgaag
      601 cggtggtgtt agcgctgctc atcgaggacg aaatgagccc cgatcaggtt gcgcaatcgt
      661 tgggtatgac gatgaacaat ctctatcaga ttcggcaccg cattcgcgcc gccgcacgcg
      721 agctgctcga cgcgtccgcg cagggggtgt aacttgcgga actctatgtc agccagtgag
      781 gaccgcgatt ttgcacgcct gagtgaactc gccgggcagg ccgccccgag cgtgagcggc
      841 ggtgagcacc tgagcgcgtc cgagctgcag cgagtacgga ccggttccga agaggaggac
      901 ttggaacact cctatcggca cctcgccacg tgtagttcgt gccgtgcacg cctcgtggaa
      961 aggccagttc taccgggcaa ggtgccgtcg cgactccgca acggcgcggt ggcggcggcg
     1021 gtggtagcgg cggcatgctt cgcggggctg tacatggcga gcacacggcc aacactgcca
     1081 gagcaactgg ccattcaaca gctatcacgg agcacactaa tgggatcgga cgtggctccc
     1141 gctgccgagg gcggaaacct ggagttgtct ttcgtcgctc cgagctccct atgcgccttc
     1201 ttgctgccgg tgggcagcga tggaacgctg ctcgccccgc tgcactggtt cgagcacgaa
     1261 ggcgcgaaac agaccctaca gctcgcgcca cgtaactttc gtgcgcacca aggaagcgcc
     1321 gaggccttga tcgtatttgg cagtgaggcc gcgacagcgg ccgtggcttc ggcctggcag
     1381 aaccacacgc cgacggcggg taaccttacc gcgcttcaga accaactagc catctgggcc
     1441 gccccgcagg gcgcacgggt gcagcgaatt gcgctgagtc agcctgcgcg tgaccatgat
     1501 tgagcgcact ctcggggcct tggatattcg cggatactgt gaacgcccct cgattcgagt
     1561 atgctgggaa gcatgagcgg attcgagaac tgggctttgc gcggagctat ccaactatcg
     1621 gtgattgccc ttgcgctagt gggctgcatg gcaccggtgg gcgtaacgac catgtcggtg
     1681 ccgcctgacg cggctgccac ctgttcatca cactgccaga ccatgggcat gcgcctcagc
     1741 gctgtcgcgg tcatggccaa caacgttggc tgcgtatgcg agccggctag gcccaacaca
     1801 cctatcgctc gaagcacccc cgcgcaggct ggagaacgaa gtaccccagt agcaggcatg
     1861 actgcactac tcatgcagca agaggcggaa gaggaagagc aagcccgtca aagcgaagct
     1921 caacgtcaac agactcgcgc acagcaacag cgacattgaa cgcagtcaat tcagtttggc
     1981 actgaacgcg tccgacttat ccggaagggc caccatgcct gcggcgcgaa tgtcgatttc
     2041 gtaagtcccg gagaccactc gtcgggtggc catcgtgaag aactgtaact gcctctcgcg
     2101 ctgaaactca gctcctcgca accccaagta ttctcgcgaa ccaagggcac acattaacga
     2161 aatctattgt tacaacccaa agcccgtgcg gtcagagaga gagaggttcc aatcatgtgc
     2221 ctgtggaagg tgtctcggga gcacaggagt gttgggacgg cgttcacgat tgttcgctac
     2281 tcgtcatcgc gagtaggtgt tgcgccgtcc gggcgcagtg cttctcggat atagcgcgaa
     2341 gtgtgccgcc gccgcgaaga ggattggaat ctgcttccgc aagactcaag gttcaccaga
     2401 cggcaactca cataagacca catctgtccc aaaagtccgg ggcttgtggt ggggatagga
     2461 cagttggcga gtgaggtctc ggcggaccac cttcgacgcc agccggaccg ctgagcttat
     2521 cgtcggccaa gccggcttcg cactaaatca gggggcttca ccggcagttc aacccgaggt
     2581 tcgagcaggg ccgcgcgccc gccatctgct cagctccgct tcgatgacac cggacggtgc
     2641 gtttctctct ccggggagtg tagcctgctg ccgtcgcggc ccgcgagcct tagcccaatc
     2701 cagtttgcgg ggtcgaagtt ttccgcgaag cgcctacact tttggcacga acggagctaa
     2761 ggacgtcagg aacgagcgcc ccagcgaagg ggcgcaaggc ctgcgccgga ctgactggct
     2821 aatcgaggtt gagaatgtcg ccgagcgtga gtgtgttccg tcgtgtggct ctgcttcgtc
     2881 gaagacgcag ggcatcggat ccgctcgtag gattgttgtt cgtgtatctc tcgtccagcg
     2941 cttgctcgct acaggcaccc aaagaggacg aggtgttcgg atcccaaggc ggcagctctt
     3001 cactccaaat gagtggcgga acgggaggtt ctggggagcc aagttctagc tccgggaggc
     3061 agggttcgag cggacagggc gctggcgctt cgacaggagg gtctggcggc actcgctccg
     3121 gttcggacga gggcggctcg ccgataaccc ctggaggtgt gaccggagcc agcggtggtt
     3181 ccaccagcgg gacttcgggc gaaacgggca gcggcggcgc tcccgcgcca gatatcgagc
     3241 gagggctcgt agcgcactat gccttcgaag aaacgagcgg ggacagcgtc gtgaacaccg
     3301 tgagcgcgtc cgcgagcggt aagtgcttcg gcacctgtct ccaccttagc gggacaatgg
     3361 gtggcgcgat cgggcttcgc aactcggaag agaacctgaa ttgggtagag ctgccgagcg
     3421 ggctcctgag tggaatgtcg gcgacgaccc tctctctctg ggtgcgcgat ctttcggcct
     3481 cccgtaagga cgctccggcg ttccacttca gtcgaggcac caacgaagtg ttcttcttcg
     3541 tgccggacga cgtagtcccc gatacctcga cctcgagcgg gtcgctgggc gtgaagcacg
     3601 gaggcgctca aatcatcagc attcggggga cccgaagtct gaccgacagg agttggcacc
     3661 acgtcgccgc cacctggacg gccgcagccg tgtccctcta tatcgacggc atgcgcatcg
     3721 gtacccaatc gagccctggt gcggtgccgg atgatttggg gccaacttct cccaattacc
     3781 tgggacgagc tttgtccgat accatcccag ccataaacgc cgatctcgat gaactccgca
     3841 tttacgaccg tgccctcgaa gccgaggaac taaacctgct gtatcggcag ccgtcgaagt
     3901 agacggggtg ctctgaatcg ttgccgcagg ggagggcccc gaaatgtcgc gcgcaagtta
     3961 acccgttctc gctcagaccg acacggacac ggttgagaac caaaccactc cgtgtgcacg
     4021 tcactactca cacccgtagg gtaaccatga gcgagatttc tgagcctgcg tatcgagagg
     4081 tccatcacca ttcacgggag agcctctcgt gaatcgcacc gccgccgcta ttgccttgct
     4141 tttggctgct ggcgcttgct cacgggagcc ctccgagtcc gccgccggca acaaaccaga
     4201 agctcaaccc gccacggggg gaatggcggg tgcctcggac cgaggtggca cggctggagg
     4261 ttcaaccgag gattcaggcg gtgccccgct tggaggtggt acgagccaac ccacgagttc
     4321 cggcggcgca aacaacgcgg gggcggcctc tattgtctcc ggtggagtaa cgggccgtgc
     4381 cggaacgagc agcgtcgaac aacaaggagg cacatccgcg ggctcgtcga gctccggcag
     4441 cgcaggctcg aatgcaggcg cgccggcggt gacgcgacct ttcgtatgcg gcccatccaa
     4501 gtcaccgatg tttcaaagcc aggagccgct cacgatgcgg attgccgcga acttcagcaa
     4561 gctgaatagc gcggccaccg ccgaagagtc gggctcctcc ggcaccatcg agctgaaggg
     4621 ggcagaaggc gtgacccagc tctcggcgaa ggtagtcgcg cgcgggaatt cccgattccg
     4681 atactgccag gtgcggccct tctcactgaa gttcaccgaa aaacagaagg ggaatttgtt
     4741 cgagcatctc ggcaagtccg tgaaattcgt atcgcactgc gtggccaagc cggatgctcc
     4801 cgtcaacgcg caagcgccgt cgctcgcagc ttacgagcag cgagtgttga tggagcacac
     4861 cgtctatcaa acgctcgatc aactgagcgt tccctcgctg aagacgcgcc tcgtgaactt
     4921 gacctatcat gacacgcaga ccgacgagga agagactcac ccggcattcg tgctcgaacc
     4981 cgaagacgaa atggccgagc gctgcggaat ggtcaaggtc gaagatttgg cgcccggcca
     5041 gggcgacaat ctgaagctca accaatacgg cgtactgtta ctacatctgc tgaacaactt
     5101 cattctgcag tatgattggg atttccagcg gaacgtcatt ccgctcgccg cgctcgatcg
     5161 cggtgagaag ttcaatggtg tatacgactt cgacttggtg gccgtgattc ggccagacta
     5221 ttcgaagaac ctgggtcgca ccctggccga gaacgcggca gggtttgcgg attggctgcg
     5281 tagaaaccaa tcccccgccc tattcaatga ggtcggctcg atgctacagc ggtccgacgc
     5341 catgctcgcc accctccagt tgccgggctt gaccgagcag aatcgcgcct tgttcactga
     5401 ctggctgcgc caattcttac ccgcgctcga gcagttcaaa gcatgcgagg gccatggcaa
     5461 tgacgccact cgtcccgcct gttatgtgcc ggacgaccac ggcgcctcac cagataccgc
     5521 aagcacacag caacctgggg agtggacgac gcgcatcgaa cctccgggcg atacggatat
     5581 gttctctctg cagctcgagg cgaatcagct ctattcgctc ggcgcctcga tgtcgctcgc
     5641 acttcgtgac gataacggcg tcctgatcgc gcaatccgaa accatcgagc ccacttcgtt
     5701 ccgagtgccc cgttccggca aattctatct ggaggcctcg atgccgggaa gctcagtcgt
     5761 ctcaccgttt cacgccgacc ctcagaagat agaccaatct gtctacctct atgcggacga
     5821 tgcgggcacc tcgctcgaca cggccgtgcc cttgagcatg gggcaagcct tcgatggcaa
     5881 gtgggaattg agtacgggtt gggaccagga ttggttcttg gtgcacgcgg agcctggttc
     5941 gtccctacat gtcacgctca atgagccgca caacgggaca cccatgctcg tcgcgcaacg
     6001 ccaggatggg tcggatgcag agcaggaact ctcgctcgag tacggcgaca acgatgcatc
     6061 gtccctgctc cccgaggccg gcgactatgt cctgaagttg agccattacg gcttcgatca
     6121 gcccggcacg taccggatcg agttcaaatg atgccgcctg cgcggtgaag ggacgggccc
     6181 tttgcggtgg cggctgcgct gacgagatgc agccgccgcg cttcgcgccc gctacacagg
     6241 tgcggccccc aatcgccggt tcgacgcctg accgtcactg aagctgaaag cagctgacga
     6301 gcagtttgtc cacctgaccg ccattcgtat gaaccgaagg cgccgcgttc agcgcgtcga
     6361 ggcagcgcgg gagcgcacac ggctcgagcc caaactctcc gggtttccga tcgcccgcgg
     6421 gaaatttgaa gaatttgttc ggctgattgc caatgaccac gaagtaacct ttgggacgca
     6481 gcagctctac ggtcgcttgg gtgatgggat ggaagccgaa tggactgatg gcgacgtttg
     6541 cataaacgga gccgagcggc agttgaacgt cgtcggcctt gcttcgttcg aagttcggga
     6601 gcgctaattc acgagcaccc gctttgggat ctggcacgtg gatcacggta ccctgaatct
     6661 tgtaggtggc gctccgcgaa ggggtggagc gcagccgctc ctcgatgaac tggagtgcca
     6721 accgctcgag cagagtcatg gcctcgatga ttggaaccag gttgggttgg tgggtaaaga
     6781 gcaagttgac gaggtgtgcg gagggcgtcg gcttctcgag aagcttcgga atcgcgtcct
     6841 gcttgtcgat gggcacgaaa agacgcacga acccgcacag ggactccagg gttctcttct
     6901 gcaggaccgt gctgaccaac atgtgaccac tgtccatgtt gaagatgacg tagtcatcgc
     6961 cctcgaggtc ggggccgaag aagcgcggaa cgagcagagt acgagggtca ctaccggaac
     7021 cgaggttcac gatgattctg agtgtcatgg ctgcgctctc acaggctgaa atggtctctt
     7081 acgaggggca gccccaccat ggagccaagc tcgtcccatc cgtcgtagac gcggtcgagt
     7141 gcggtcgccg cgttgttgtg cgcgccgtcg gtatcgttgg cggccaagcc ctgctgctcc
     7201 gcttgcaggg tggcaaatac atccgagccg agcgttacgc cgatcacatg ggtgtttccc
     7261 ttgactcgat tgaccgcaaa cagagggccc cccgagtccc cggggagagt cgtccaagcg
     7321 gcagatgatt cgagcacgag caccgacgta ttctcgccag cggtggtatc gtagtaggtc
     7381 gcgggacgcc cgttgctgaa cttggagagc ttgaagttta gatttccagc gctgtccggg
     7441 ttatcggcac gggtccgacc atagccggct tgcaacagct cccaacccac cggcagggtg
     7501 ccatgcttga tcagttccag catcagctcc ttggcttctt tgagctgaga
//
LOCUS       DASTKU010000355         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_68110,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000355 DASTKU010000000
VERSION     DASTKU010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68110"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFQ61_12295"
     CDS             complement(<1..641)
                     /locus_tag="VFQ61_12295"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9955282.1"
                     /translation="MRRVFLIARRELFAYFRSPLGAVILAGALLIDGVYFYWKGLSQK
                     QVSAEVLREFFNGTSGVIMAASLFLAMRLFAEERQTGTLVLLNTAAIKDWEIVLGKFL
                     SAFLVIALLTVLTAYMPALILISGKVSAGHVAVGYLGILLIGSAAISIGLFGSALARS
                     QVVAIIVSAALLAVMLLLWAVARSVDPPLNRYIAGLALHHDNFRPFMQGRLELG"
     gene            complement(638..1675)
                     /locus_tag="VFQ61_12300"
     CDS             complement(638..1675)
                     /locus_tag="VFQ61_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9955283.1"
                     /translation="MIEIQDLYKYYGDRRAIGPLTVNIESGEIVGLLGLNGAGKTTTL
                     RILACDLMPTSGSVKVGGHDVVAEPDAVRARTGYLPDRPPLYDDMSVVEYLGFAAQLR
                     GVSGDQVRRRVDDVLELTELGFVRNQIIGTLSHGYRQRVGIAQAIVHRPEFVVLDEPI
                     SGLDPVQIVEMRELVRSLRGEHTVIVSSHILTEISETCDRILVISDGRIAWGGTEKQL
                     FADFGRGMQVAITTRGAAAPRLEQLLRAVPGVSRVELVTPSEPGDEVVTVLLSAEGEV
                     RDAACRTLVQHDVAVLGVARQQGLENMILKLLGGGEDAQARRSRKKNSEVKPSESDSD
                     PAGAETNAEGA"
     gene            1998..>2759
                     /locus_tag="VFQ61_12305"
     CDS             1998..>2759
                     /locus_tag="VFQ61_12305"
                     /inference="COORDINATES: protein motif:HMM:NF019931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueG-associated DUF1730 domain-containing
                     protein"
                     /protein_id="HET9955284.1"
                     /translation="MPNQTKLAPPDPAEGVSENPDCESRAPEGIEHFMPSAGDSPPML
                     PRELTPIELTRELQGWAPELGFERLGVTSVEPMEAGRRRLEVFREQGYAGGLDYLMTG
                     SRHEPRRLMPSARSVVVALMAYSGPQAASEQDLIGRVARYARGEDYHIVLKQRLLALG
                     RRLADLVQRPLLGRACVDTAPLLERELAARAGMGFQGKSTMFIAPGTGSYLLLGELLL
                     ELELAPTTGASSGCGQCRACLDACPTQAFPSPYVLD"
BASE COUNT          505 a          932 c          846 g          476 t
ORIGIN      
        1 ccgagctcta gccggccctg catgaatggc ctgaagttgt cgtggtgcag ggccagccct
       61 gcaatgtatc gattgagcgg cgggtccacg gacctggcga cggcccagag caagagcatg
      121 accgcaagca gcgccgccga cacgatgatg gcgaccacct gcgaacgagc caacgccgaa
      181 ccaaacagcc cgatcgagat cgcagcactg ccgatcaaga gaatgcccaa gtaaccgacc
      241 gcaacgtggc ccgcgctcac cttgccgcta atcaggatga gagccggcat gtaggcggtc
      301 aacaccgtga gtagcgcgat caccagaaat gccgagagga acttaccgag aacgatctcc
      361 cagtctttga tcgccgcggt gttgagcaac actagcgtcc cggtctgccg ctcctcggcg
      421 aacaggcgca tcgccaggaa caaagaggcg gccatgatta ccccgctcgt tccattgaag
      481 aactcgcgca gcacctcggc cgaaacctgc ttttgggaca ggcccttcca atagaaatac
      541 acgccatcga tcagcagcgc gcccgcgagg atgacggccc ccaaaggcga cctgaagtaa
      601 gcaaacaact cgcgtctcgc gatcaaaaac acgcgcctca tgcgccctcc gcattggtct
      661 ccgcaccagc cgggtccgaa tcggattcgc tgggcttgac ctccgagttc ttctttcggg
      721 agcggcgcgc ctgcgcgtct tcgccgccac ccaagagctt caagatcatg ttctccaagc
      781 cttgctggcg tgccacgccc agcaccgcca cgtcgtgttg cacgagcgta cggcaagccg
      841 cgtcccgaac ttcaccctcg gcgctgagca acacggtcac cacctcgtcc ccgggttcac
      901 tcggcgtcac gagctccaca cgcgatacgc ctggcactgc gcgcagcagc tgttcgagcc
      961 gtggtgcggc ggcaccgcgc gtggtgatcg cgacctgcat gccgcgcccg aaatccgcga
     1021 acaactgctt ctcggtgccg ccccaggcga tgcgcccatc gctaatcacg aggatgcgat
     1081 cgcaggtctc gctaatctcg gtcaagatgt ggctggagac gattacggtg tgctcgcctc
     1141 tcaagctgcg cacgagctca cgcatctcca cgatttgcac cggatcgagc ccggaaattg
     1201 gctcgtccag gaccacgaac tcgggacgat gcacaatggc ttgcgcgatg ccaacccgct
     1261 gccgatagcc gtgcgacagc gtgccgatga tttggtttcg tacgaagccg agctcggtca
     1321 gctccagcac gtcatccacg cggcgccgga cctgatcgcc cgagaccccg cgcagctgcg
     1381 ccgcgaatcc cagatactcg actacgctca tgtcgtcgta gagcggcggt cggtcgggca
     1441 ggtaaccggt gcgtgcacgc acggcatccg gctccgcaac cacgtcgtgg cccccgacct
     1501 tgacgctgcc actcgtgggc atgaggtcac aagccaagat gcggagcgtc gtggtcttgc
     1561 ccgcgccgtt cagccccagc agtccaacga tctcacccga ttcgatgttg accgtcagag
     1621 gcccgatggc tcgtcgatcc ccgtagtact tgtagaggtc ctggatctcg atcacgttcg
     1681 tcccacgcta ggttgtagag cctgtgtgtt gttgagagcc tgtgttgtga gagcctgagg
     1741 tcgttgctga gagcctgagt ggcgcttggc cacccggttt ggtgcccggt ccgtatggtg
     1801 gccccagctc tgcgtagacc tccaccggca ccgcggttac cacaaagcgc ctggccccgt
     1861 taccacaaag gcacggccgg ggtgggacca ggaaccggcg caatttattc cccgctcgtc
     1921 cgattcccgt cctggttcgt tctttccacg tttttcgacc ggcgtcggct tgtccgtcgg
     1981 ccaaaatggg ccgcacgttg ccgaaccaaa cgaagctagc tccacccgac ccggcggagg
     2041 gcgtatccga gaaccccgac tgcgagagcc gagcgcccga aggaatcgag cacttcatgc
     2101 cgagcgcggg tgattcgcca ccgatgttac cgcgcgagct gacaccgatc gagctcacgc
     2161 gggaactgca aggctgggca cccgagctcg gattcgaacg gctgggcgtc actagcgtgg
     2221 agcccatgga agccgggcgc cgacgcctcg aggtgttccg cgagcagggc tacgcggggg
     2281 gcttggatta cctgatgacc ggctcgaggc acgagccacg ccggctgatg ccctcggccc
     2341 ggagcgtggt agtggcgctg atggcctatt cgggcccgca ggctgcgtcc gagcaggatt
     2401 tgattgggcg cgtggctcgg tacgcgcggg gcgaggacta ccatatcgtc ctcaagcagc
     2461 gcctgcttgc gctcggacgc cgcctcgccg acctcgtgca gcgccccttg ctcgggcggg
     2521 cctgcgtgga cacggccccc ttgctggaac gcgagctcgc cgcgcgcgcg ggaatggggt
     2581 ttcagggcaa atcgacgatg ttcatcgccc ccggtaccgg cagttacttg ctcttgggcg
     2641 agctactgct ggagttggag ctcgcgccga ccaccggcgc gagctccggt tgcggtcagt
     2701 gccgagcctg cctggacgcc tgtccaacac aagccttccc aagcccgtac gtgctcgac
//
LOCUS       DASTKU010000356         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_68218,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000356 DASTKU010000000
VERSION     DASTKU010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68218"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..2310
                     /locus_tag="VFQ61_12310"
     CDS             352..2310
                     /locus_tag="VFQ61_12310"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HET9955285.1"
                     /translation="MGRREFYSAMGLVRRRGRSRGRAWMLGAMVAYGVIGCSGDKEES
                     PSCEAGKEGCACYPNNSCNEGLSCLSKLCVDSGVDSSGGRGGSTSGGGSLQQGGEGDS
                     SSLGGSAGSGGTARGGGGGQGGSPTNGGSAADGGTQARGGAQQGGAQQGGAQQGGAAN
                     TPVQRYGALSVNGANLVDEQGKSVKLEGISSMWLNWDPTGYAQSKAALQYMRDSWNLR
                     VFRAAMGVDAEGAYLLDPDTAKAQVNRIVQNAIDLGVYVIIDWHDHEALAHADQARAF
                     FDEMSKKWGEYPNVMYEVFNEPLDLDWGSQLKPYHASLVDVIRKNDPDNIILLGTPNW
                     DQDVDVAAQSPLAGKNLMYTLHFYACSHAARERSKAQTARSRGLPMFVTEWGATHADG
                     GVDGVVCEGEARTWHDFLDQHAISWAAWKMDGCTDSSCLFKNRDVPVDGGWTAQQLNG
                     HAPLVIDEMKTGPESLAGTGGGGSGGSTSMGGASSGGTGGTAGGTTSFPPDPAGCELV
                     TSCTDCCETTGVYGLDPASMDATGRLVKSFSVEGGQARATFQFSELDEVGGIFFKLTS
                     PRSIESIGLTAGGDGDAFEVALVNGSGANGCLYDLSSAGLASEPYTCWENGAGPSAGE
                     PVDQIEVRVRALQSGPASLVVQELEFGP"
BASE COUNT          503 a          784 c          959 g          475 t
ORIGIN      
        1 ccatgactta cgcgatgcgc ctgtatgaga acgcctttgc gttcctacgc atgggctatg
       61 ccgcggcgat ggcgtggatc ctgttcttga tcgtcctcct cctgacttgg ctggcggtgc
      121 gtgtcgcgag ccgccgcgtc cattacaccg gaacctagtg tccagctgga tgcggtacgg
      181 gtgtaggttt gcgactcgga gctcgcgagc gagcggtgga ggcgtgggcg cgccatgccg
      241 atacccggtg ctcgctaggc acaggctttt cggctagagt gctcggcggg tagacagcac
      301 tccagcttgc gccgaagcaa gggacgagtg cggaagtttg ggaaggtatt catgggtagg
      361 cgagaatttt attctgcgat gggtttggtg cggaggcgtg ggcggtcgcg cgggcgcgct
      421 tggatgctcg gagccatggt ggcgtacggg gtgatcggct gtagcggcga caaggaagag
      481 agtcccagtt gcgaggccgg gaaagaaggc tgtgcctgct accccaataa ctcgtgcaac
      541 gaagggctca gctgtctctc gaagctgtgt gtggattccg gcgtagactc gagcggcggc
      601 cgtggtggaa gcacgagcgg aggaggcagt ctccagcaag gcggagaagg cgactcgagc
      661 tcattgggtg ggagcgccgg cagtggtggt acagctcgcg gtggcggagg agggcagggc
      721 ggtagcccca caaatggcgg tagcgccgcg gatggcggaa ctcaggcgcg cggtggggcg
      781 caacagggtg gcgcgcaaca gggtggcgcg caacagggtg gggcggccaa tacgcccgta
      841 cagcgctacg gcgcgctctc ggtcaacggc gccaacttgg tggacgaaca aggcaaatcc
      901 gtgaagctcg aagggatcag tagcatgtgg ctgaactggg atcccacggg gtacgcgcaa
      961 agcaaggccg ccttgcagta catgcgagat agctggaacc tccgggtgtt tcgggcggcc
     1021 atgggcgtcg acgctgaggg tgcgtacctg ctcgacccag acacggccaa ggcacaggtc
     1081 aatcgaatcg tgcagaacgc gattgatctc ggcgtctacg tgatcatcga ctggcacgat
     1141 cacgaggctc tggcgcacgc cgatcaagcg cgagccttct tcgacgaaat gtcgaagaag
     1201 tggggcgagt accccaacgt gatgtacgag gtcttcaatg aacctctgga cttggactgg
     1261 ggcagccaac tcaagcccta tcatgcgagc ctggtggacg tgatccgcaa gaacgatccc
     1321 gacaacatca tcctcctcgg cactcccaac tgggaccaag acgtcgacgt tgcggcgcag
     1381 agcccgctag caggcaaaaa cctgatgtac acgctacact tctacgcctg ctcacatgcc
     1441 gcgcgtgaac ggagcaaggc gcaaacagct cgctcccgtg ggctaccgat gttcgtgacg
     1501 gagtggggcg ccacccacgc ggacggaggc gtggatgggg tcgtgtgcga aggtgaggcg
     1561 cgcacgtggc acgacttttt ggatcaacat gcgatcagct gggcggcttg gaagatggac
     1621 ggctgcacgg atagcagctg cttgttcaag aatcgcgacg tgcccgtgga cggtggctgg
     1681 acggcgcagc agctcaacgg ccacgcgccc ctcgtgattg acgaaatgaa gacggggcca
     1741 gagtcgctgg cagggacggg cggaggcggc agcggcggca gcaccagcat gggtggagcc
     1801 tccagtggcg gcaccggcgg tacggcgggc ggcacgacct ccttcccgcc cgacccagcg
     1861 gggtgtgagc tggtgacatc ctgcaccgac tgctgtgaga cgaccggtgt atacggcttg
     1921 gatcccgcgt cgatggatgc aaccgggcgc ttggtgaaga gcttctccgt ggaaggtggc
     1981 caagcgcgcg ccaccttcca gttctccgag ctggacgagg tcggcggcat cttcttcaag
     2041 ctgacgagcc cgcgtagcat cgagtccatt ggtctcaccg cgggcggcga tggtgacgct
     2101 ttcgaggtgg ccttggtgaa cggctcgggc gcgaacggat gtctctacga tctgtcgagc
     2161 gcggggctcg cttcagagcc gtacacgtgc tgggaaaacg gagcggggcc ttcggcggga
     2221 gagcccgtcg atcagattga ggtgcgagtg cgcgcactgc agagcggtcc cgcatcgctc
     2281 gtcgtgcagg agctcgagtt tggcccctga acgctagagc tgccccgcgg gttcgattga
     2341 gcaaataccc ccgcagcgtg cctgcaacac gctgcgggag tcaccccgag gcgtctcgat
     2401 catccagcgc tcagtctccc caactcgcgc cggaagccgg ctcgctgcag aacggcgacg
     2461 cctctgatag tcgtttgatc ctgaccgttt ccggttcgag ggccgcacgg cgcggatctc
     2521 gcctttggcg ctgagatggt ggtaagcgaa agccatgtcg aggcccccgg cacacctccc
     2581 cttgcgtttt tacggacgca cgccgagagc cgccgcgccc attacgccgg agcatagtcg
     2641 ctcgtgagtg caggcacttc cccgttcagc agcagctttc gaagaaatcc gtggcggcga
     2701 gcctgggttc atatcgcgct c
//
LOCUS       DASTKU010000357         9510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_290183, whole genome shotgun sequence.
ACCESSION   DASTKU010000357 DASTKU010000000
VERSION     DASTKU010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290183"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VFQ61_12315"
     CDS             <1..956
                     /locus_tag="VFQ61_12315"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HET9955286.1"
                     /translation="WVEAAPDAPDAKRRALARIDALAKPSAILVSTTKFLDLESLAAV
                     TSRPGDLVGLHFASPIEQLKLLECARTPLTLPDALATVMKLGRALGKVTVAVRGHVAH
                     RLRAPYLREALFLLEEGALPQEVDLTLTEFGFRTGPFAALDLLGYEEILEERRVRFAQ
                     LTAREQACTLLEELTRLGRTGRASGLGFYRYEGGRAAPDPSIVPLLERHSKARATARR
                     AIAPEEIRERCVFSVINEAARILAEGVAARPLEIDLIGVHGFSFPTYRGGPLFFADQL
                     GLDQVIQRMQRLREQLGEEYWTPAPLIERLRAGGQGFYVGS"
     gene            970..3039
                     /locus_tag="VFQ61_12320"
     CDS             970..3039
                     /locus_tag="VFQ61_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HET9955287.1"
                     /translation="MGSARIHLERLFAPESVAVVGASAAPEKAGYQAMLALAGFAGEV
                     FAINAKADVVLGRKAYPSLRALGRPVDLVVFAIPAAACVSAVREAIEIGCGGGLVVSG
                     GFSESGPEGSVLEAELGRVCRESRFRLLGPNTAGFVNKAISLTASFLPSAERIPLGHI
                     AVVAQSAGINLTVSFLIAKLGHGVSCAVGLGNSVDIDAADTLEFAARQPATRAIALHL
                     EGLRNGRRLYETIRRVSPLKPVVVFTVGKTEIAEFARSHTGNLIGSYELRRSALEQAG
                     AVLVESTEELAVAAAALSSHRLPAKRHPGVGVITAQAGAGLAILDLLQSKHTSVPPLT
                     AVTEARLAELLPPMTYQRNPVDTGRPGSSFGEVVSTVAQDDAVDAVIVYALSEPAALR
                     PAESLPAVQHEVAKPILFGTMGPPDEVEETSEALRALGFYVAHSPEELARAANALTRD
                     ATLQAHLARKQERPLLAKTAHLEGPPDEHTAKRVLEALGVATPRRAACATREAAHDAF
                     RSLVKPVVAKILTSEIEHKTEVGGVRLNIHDDASLDVALAGLDAIPLASTRRYLIEEM
                     APPGLELIVSVRRDPSFGPVVALGLGGTLAELLRDTATRLAPLTKTEAEEMLDELRAA
                     PLFAGWRGSPKLDRTAVVDCLVAIGDFICQEPGVDELEINPLRVYPTGVLALDALLIV
                     GQNRAAE"
     gene            complement(3090..4058)
                     /locus_tag="VFQ61_12325"
     CDS             complement(3090..4058)
                     /locus_tag="VFQ61_12325"
                     /inference="COORDINATES: protein motif:HMM:NF006015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9955288.1"
                     /translation="MPASPKKKNVRALSSGRSAPAAKRSRVKHATAEVSSGASPIDLH
                     QVLAQRVRDARARRFMTRKALAQESGISLAYLARVERGSGNISLSLLQRLALALNLPI
                     ESFLSADHAPNADLTMIIEFLKRQPPERLALIRRQLFESHDQAQRIALVGIRGVGKST
                     LGPALAERLCIPFVELNREIEKEAGLGVSEIFTVYGQHGYRVIERRCLERIIVNHPKV
                     VLATAGGIVAETATYELLLNSFFTIWLKASPEVMFERVLAQHDARIASPELRTEAIEN
                     IERTLGARQHLYELAPAAFDTSGKTVEQIVRGLLGLLPAGVREPLV"
     gene            complement(4247..5035)
                     /locus_tag="VFQ61_12330"
     CDS             complement(4247..5035)
                     /locus_tag="VFQ61_12330"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HET9955289.1"
                     /translation="MSKQACTAWLEVGHEFAEFELEEVAWSGQELERLGRLVRERLAH
                     DSDLPRAARHVVFEQLSFVREVDDASLRFVLLPSVLRLRRGNCVGLGTLLVSLIQLAG
                     GDARGVLRPGHFHVRVRHQNVARNLEPLRGGEEMPDDWYESRFPFVGPVSYYARSLSS
                     NEVLGVLEYNIGNERRKGQRLGEAERAYTRAVSHFPEFAEAHASLGTVLHVQGKLGAA
                     ERAYRKALRMNRTLPGLEWNLELLLAERERAGTAQSAPGGLRGR"
     gene            complement(5095..7122)
                     /locus_tag="VFQ61_12335"
     CDS             complement(5095..7122)
                     /locus_tag="VFQ61_12335"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955290.1"
                     /translation="MTLQSKRAVVLTTLLVSCHPAARYPWTAPSRNAPFEHAEVRTLN
                     EDYQRTNAVVLLREVQTPTRAALDYAAYHANFIIADPEPEGELDPAAKPTFDQPAPFG
                     GVVDKSTLSADLYTTPLTESERTHPLSLAPMLEFLHSSKVDGAKELEQAGAQVRDEAW
                     GLPRRPGISNQSIAEVFVHASGGSRELWAKIEFQPWLKRAWSLPDQDGDGVPELYGRL
                     DPSVIKPAIWSSIEDDYRKRRLSREEVGTWANRLASYWYPSFNTDLVPPGTAWPDSHT
                     EAEVKKSLGERSYPAPTIVLRGKPQGNATYQVFFVKGLGDGESQPARAAGPALKLTKT
                     APTPQPDATRAAIERELREHGGSFERWAQSTAPAAESLRRHARSLRGKVKALEGKDGF
                     LFFKNSLEYAAAPELERQKKGKNPLPIIVEFQKALAAQGVDFLFVPVPTKVEVYPDRL
                     DPKLASYAGQVVNPQGRKFLLALANAGVEVVDLLPAFLAARRAQSGELVFQRQDTHWT
                     DSGLRLAADILSERIKKYPWYKELSRHAQRFTTREAKFQRLGDLHSRLPDAVKSRYEP
                     EQLIAQQVIGSNGEPYVDDPDSPIVVLGDSFTGVYQLMDAEHAGLSAHMAKDVGYPID
                     LVMSYGGGPNVRHKLMRRGTDALESKKLVVWVMTARDLYNYWEDWEPLGGK"
     gene            complement(7153..7932)
                     /locus_tag="VFQ61_12340"
     CDS             complement(7153..7932)
                     /locus_tag="VFQ61_12340"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9955291.1"
                     /translation="MHLAIVIPAYNAERKLEGVLDRIARVPLSCTKTIIVVNDGSRDG
                     TLGLLDDLIRRTDLAPLQVIHRAQNGGYGAAMQDGLEHARISQADVVACVHADGQYSP
                     EVLPMLLEALTARRLDILQGSRIAAGTALSGGMPLYKYIGNALLNQLENLTFGLDMTD
                     YHSGYLLYSRRALEVLSFGQLSKSFDFDLEVIASARAHGLAVAEAPVPTHYGDEISYL
                     NPVSYGLRVLRVLWRYQRGVYGPRPASNRGSALATDAPQRA"
     gene            complement(7934..9049)
                     /locus_tag="VFQ61_12345"
     CDS             complement(7934..9049)
                     /locus_tag="VFQ61_12345"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9955292.1"
                     /translation="MAAARIAVLGAGGFLGSHLVPRLLELGDVQVYAIDQDLHKLECR
                     DPRVKRRVARLEQPGLIEEVTAQSSVILSLTALCTPALYNTDPLAVIDANYTELLPLV
                     RASARHGARLIHFSTCEVYGRRSLDLTGLPRETMNEEANALFLGPVHNERWTYACAKQ
                     LLERVIYAHGAHGNLNYTIVRPFNVIGPRMDYMPGVDGDGVPRVLACFMAALLRGEDL
                     QLVDGGQQRRSFIAVDEFCAGVLRIVERGEACREEIINLGNPQNDVTIRELARALADE
                     YAGYTGQPRARLRDVSAAEFYGAGYDDTPVRVPDISKARRLLGFEPCTSLAEMLPGIV
                     RDYVRRYGQTSSYDQTSSYGENTIRPANPAKFALKAV"
     gene            complement(9049..>9510)
                     /locus_tag="VFQ61_12350"
     CDS             complement(9049..>9510)
                     /locus_tag="VFQ61_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955293.1"
                     /translation="DGKYLTITLRGSQRETGIWDIQGKRWHKTGLGCQVSFTPDNASI
                     YWVNPTGNGGSEVFMMPVTNGAASKDLSDDQLKFMDLPGRRSHEYFPRLSRDGKWMTW
                     GATQRGHDHDIADYEIYLWEVGTPADGAVRLTFHSGNDRWPDIHLDDEGGR"
BASE COUNT         1659 a         3057 c         3013 g         1781 t
ORIGIN      
        1 tttgggtcga agccgcccct gacgcgccag acgcgaagcg ccgtgcgttg gcgaggatcg
       61 acgcgctcgc gaagccatcg gccattctgg tgagcaccac caagttcctc gacctcgagt
      121 cgctggcggc cgtgaccagc cgacctgggg acctggtggg gctgcacttc gcgagcccca
      181 tcgaacagct caagctgctc gagtgcgcgc gtactccgct caccctcccg gacgccctcg
      241 caaccgtgat gaagcttgga cgcgcactgg gaaaggtgac ggttgcggtg cgcggccatg
      301 tcgcccaccg gctgcgtgca ccttacttga gagaggcact atttctgctc gaagagggcg
      361 cgttgcctca agaagtcgat ctcacgctga ccgagttcgg cttccggaca ggtccgttcg
      421 ccgccctcga tctcctaggt tacgaggaga ttctggagga acggcgagtt cggtttgcgc
      481 agctcacggc gcgtgaacaa gcctgcactc tgctcgagga gctcacccgg ctgggtcgca
      541 ccggtagagc ttcggggctt ggcttctatc gctacgaagg tggtcgggca gcgcccgatc
      601 cctcgatcgt gccactgctc gaacgacact cgaaggctcg agccacggcg cggagagcca
      661 ttgcccccga ggagattcga gagcgctgcg tcttcagcgt catcaacgaa gcggcgcgca
      721 tcttggcgga gggggttgca gcgcgaccgc tcgagatcga tctgattgga gtccacggct
      781 tctccttccc aacgtatcgc ggcgggccac tgttcttcgc ggatcagctg gggctcgacc
      841 aagtgattca gcgcatgcag cgcttgcgcg agcagctggg ggaggagtac tggactcccg
      901 cgccgctcat cgagcgcttg cgggcgggcg ggcaaggctt ctacgtcggg tcctgaaagc
      961 aaaccgccaa tgggatcagc tcgtatccac ctggagcgcc tgttcgcacc agagtccgtc
     1021 gcggtggttg gagcctcggc tgcgcccgag aaggcgggct atcaggcgat gctcgcactg
     1081 gccggctttg ccggcgaagt ctttgctatc aatgcgaagg ccgatgtagt gctggggcgt
     1141 aaggcgtacc cctcactgcg ggctctcggg cgccccgtag acctggtggt cttcgcgatt
     1201 cccgcggcgg cttgcgtcag cgcagttcgc gaggccatcg aaatcgggtg cggaggtggc
     1261 ctggtggtga gcggagggtt ctccgagtca gggccggaag ggagcgtgct cgaggccgaa
     1321 ctcgggcgcg tttgtcgtga gtctcgcttt cgtttgctgg gccccaacac ggctggtttc
     1381 gtcaacaagg ccatctcgct cacggccagc tttttgccat cggctgagcg gatcccgctc
     1441 gggcatatcg ccgtggtcgc gcaaagcgcc ggtatcaatc tcacggtcag cttcctaatc
     1501 gcgaagcttg ggcacggggt gtcttgcgcg gtggggctcg gcaactcagt ggacatcgac
     1561 gccgccgata cactcgagtt tgccgcccgt cagcccgcca cccgagccat cgccctgcat
     1621 ctggagggac ttcggaacgg gcgtcgtctg tatgagacca tccggcgcgt gtcacctctc
     1681 aaacccgtcg tggtctttac ggtagggaag acggagatcg cggagttcgc tcgatcgcac
     1741 actggaaact tgattggatc gtacgagctt cgccgcagcg ctctcgaaca agcgggagcc
     1801 gtgctcgtag aatctacgga ggagctcgcg gtggctgcgg ccgcgctctc gagccatcga
     1861 ttgccggcca agcgccaccc aggagttggc gtgattacgg cgcaggccgg tgcgggcctc
     1921 gcgattctgg atctgctcca gtccaagcac acgagcgtgc cgccgctcac agccgtcacc
     1981 gaggcccgcc tggccgagct acttcccccg atgacctacc agcggaaccc ggtagacacc
     2041 gggcgccccg gctcttcttt cggtgaggtg gtctcgacgg tggcgcagga cgacgcagtt
     2101 gatgcggtga tcgtctatgc gctcagcgaa cctgccgcac tgcgacccgc cgagagcttg
     2161 ccggcggtcc aacacgaagt cgcgaagccg attctgttcg gcaccatggg cccccccgac
     2221 gaggtcgaag aaaccagtga agcattgcgg gctttgggtt tctacgtggc gcactctccg
     2281 gaagagctcg cgcgcgccgc gaacgcgctg actcgagacg cgacgttaca agcacactta
     2341 gcgcgaaagc aggaacgtcc acttctcgcg aagaccgcgc acttggaggg cccgcccgat
     2401 gaacacacgg caaaacgagt gctcgaggcg ctgggagtgg cgacgccgcg ccgcgcggct
     2461 tgtgccacac gagaggccgc gcacgacgcc ttccgtagtc tagtaaaacc ggtggtggct
     2521 aaaatcctga cttcggagat cgagcacaag accgaggtcg gcggggttcg actgaacatc
     2581 cacgacgacg cgagtctcga cgttgccctg gcgggcttgg acgcgatccc cttggcctcg
     2641 acgcggcgct acctgatcga agaaatggcc cctcctgggc tcgagctcat cgtctccgtg
     2701 agacgagatc caagcttcgg gccagtcgtc gcgttgggcc tgggcgggac gctggccgag
     2761 ttgttgcgtg acaccgccac gcggctcgcg ccgttgacca agaccgaggc ggaggagatg
     2821 ctcgacgagc tccgcgccgc gccgttgttt gccggttggc gtgggagccc gaagctcgat
     2881 cgcacggcgg tcgtggactg tctcgtcgcg atcggcgatt tcatttgcca ggagcccggc
     2941 gtggacgagc tagagatcaa cccgctgcgc gtctatccaa cgggtgtgct ggcgctggac
     3001 gcactgttaa tcgtcgggca gaaccgggcc gctgagtagc agccgaaccg ctgaactcag
     3061 cctcttcgcc ggggccacgt gagcacagct caaaccagcg gctcacgcac gcccgccggt
     3121 aacagaccca acaggcctcg cacgatttgc tcgaccgtct tgccactcgt atcgaaggcg
     3181 gccggcgcga gttcgtagag gtgctgccgc gctccgagcg tgcgctcgat attctcgatg
     3241 gcttcggtgc gaagctctgg gctcgcgatg cgcgcatcgt gctgggcgag cacacgctcg
     3301 aacatgacct ccgggctcgc cttcaaccaa atcgtgaaga aggagttcag caggagctcg
     3361 taggtggcgg tctcggctac gatgccgccg gccgtcgcga gtacgacctt tgggtgattg
     3421 acgatgatgc gctcgagaca gcgtcgttcg atcacgcgat acccgtgttg cccgtaaacc
     3481 gtgaagatct cactcacgcc caagccggcc tctttctcga tttcccgatt gagctcgacg
     3541 aacggaatgc acagccgctc ggccagcgcc gggcccaagg tcgacttgcc gaccccgcga
     3601 atccccacca gcgcgatgcg ctgggcttgg tcatggctct cgaacagctg gcgtcgaatc
     3661 aacgccagtc gctctggcgg ttggcgcttc aggaactcga tgatcatggt taggtcagcg
     3721 ttcggggcgt gatccgcgct caggaagctc tcgattggga ggttcaaggc cagcgctagt
     3781 cgctgcaaca ggctgagtga gatgttgccg ctgcctctct ccacgcgcgc gaggtacgcg
     3841 agcgaaatcc cggactcttg ggcgagcgcc ttgcgcgtca tgaaacgtcg cgcgcgggcg
     3901 tcgcgcactc gctgcgccag aacctggtga agatcgatcg ggctcgcgcc ggaactcact
     3961 tcggccgtcg cgtgcttgac ccggctgcgt ttcgccgcgg gcgcgctgcg ccccgaactc
     4021 agggctcgta cattcttctt cttgggcgat gccggcatta cggctgcaag cgtagtggct
     4081 gagaccggcg ctgtcgcggt agcgcgcggc gcacgcttcg ggtaggccgg cctgctcggg
     4141 gcgggttcgc ccactccctg gctgatcgct cgtcgacctc cgacgccttc tcgtcaggcg
     4201 atcgggcccg cgtatcagtc gagcctatca gcagggcgcc gcggccctat cggccccgaa
     4261 gtccgccagg cgccgactga gccgtcccgg ctcgttcccg ctcggcgagg aggagttcga
     4321 gattccattc gagccccggc agggtgcgat tcatccgcag agccttgcga tacgcccgct
     4381 ctgccgctcc gagcttgccc tggacgtgca gcaccgtgcc aaggctggca tgcgcctcgg
     4441 cgaactctgg gaagtgactc accgcgcgcg tataggctcg ctcggcttca ccgaggcgct
     4501 gtcccttccg acgctcgttg ccgatgttgt actcgagcac tccgagaacc tcgttcgagg
     4561 acagtgagcg cgcgtagtag ctgaccggtc ccacgaacgg aaatcggctc tcgtaccaat
     4621 cgtccggcat ctcttcaccg ccccgaagtg gctccagatt gcgcgcaacg ttctggtgcc
     4681 ttactcgcac gtgaaagtga ccgggccgaa gcacaccgcg agcgtcgccc ccggcgagct
     4741 gaatcaagct gacgagcagg gtgccgaggc cgacgcaatt gccgcgccgc agtcgcagca
     4801 ccgacggtag cagcacgaag cgtaaactcg cgtcgtccac ctcgcggacg aagctcagct
     4861 gttcgaacac cacgtgccgc gccgcacgtg gcaggtcgct atcgtgcgca agtcgttccc
     4921 gcaccagacg gcctagtcgc tcgagctctt gcccgctcca agcgacctcc tcgagctcga
     4981 actcggcgaa ctcatggccg acttccaacc aggccgtgca agcctgtttc gacacgggcg
     5041 ctggcgctct cgagcacccc gcgaccagcg cgagccgaat cagcgcccgt cgattcactt
     5101 gcctcccagc ggctcccaat cttcccagta gttgtagaga tcgcgcgcgg tcatcaccca
     5161 gacgacgagc ttcttactct ccagcgcgtc ggtcccgcgt ctcatcagct tgtggcgcac
     5221 gttcggacca ccgccatagc tcatcaccag atcgatcgga taaccgacat ccttagccat
     5281 atgcgccgat agacccgcgt gctcggcgtc catgagttga tagacacccg tgaagctatc
     5341 accgagcacg acgatagggc tgtccggatc gtcgacgtaa ggctcgccgt tgctcccgat
     5401 gacctgctgc gcgatcagtt gctccggctc atagcggctc ttcaccgcgt ccgggagacg
     5461 cgagtgcaga tctccgagcc tttgaaactt cgcctcgcgc gtcgtgaagc gctgcgcatg
     5521 acgcgagagc tccttgtacc aggggtactt tttgatccgt tcgctcagta tgtcagcggc
     5581 gaggcgaagc ccggaatccg tccagtgcgt atcttggcgt tgaaaaacga gttcccctga
     5641 ctgagcccgc cgggccgcga gaaacgcggg cagtagatcc accacttcga cgccggcgtt
     5701 ggccaacgcg agtaggaact tccgaccctg agggttgacc acttgccccg cataactggc
     5761 gagcttcggg tccaagcgat ccggatagac ttccaccttg gtcggcaccg gcacgaacaa
     5821 gaagtccacc ccttgtgcgg ccagcgcctt ctgaaattcg acgatgatcg ggagtgggtt
     5881 cttacccttc ttctgtcttt cgagctcggg cgcggccgcg tactccaggc tatttttgaa
     5941 gaagagaaag ccatctttgc cttcgagggc ctttactttg ccgcgcagtg agcgcgcgtg
     6001 tcgtcgcaga ctctccgcgg ccggtgcggt cgactgggcc cagcgctcga aggagccgcc
     6061 atgttctcga agttcgcgct cgatcgcggc gcgggtcgca tcgggctgtg gtgtaggcgc
     6121 cgtcttcgtc agcttgaggg cagggcccgc ggcgcgtgct ggctgcgatt cgccatcacc
     6181 caaccccttc acgaagaaga cttggtaggt cgcgttgccc tgcggcttgc cccgcaacac
     6241 gatggtgggg gcagggtagc tgcgttcccc caagcttttc ttgacctctg cctccgtgtg
     6301 cgaatcgggc cacgcggttc ctggcggaac cagatccgta ttgaaggaag gataccagta
     6361 cgacgcgagg cggttcgccc aagtgccgac ctcctcccgg gataaccgac gcttacggta
     6421 gtcgtcctcg atgctcgacc aaatcgcagg cttgatcacg gacggatcga ggcgaccgta
     6481 gagctctggc actccgtccc catcttggtc cggtaaggac caagctcgtt tgagccaagg
     6541 ttggaattcg atctttgccc acagctcgcg gctcccaccg ctcgcgtgca cgaacacctc
     6601 ggcaatgctc tgattcgaaa tgcccggacg gcgtggaagc ccccaggctt catcccgcac
     6661 ttgcgcgcct gcctgctcga gttccttcgc cccatccacc ttgctagaat gcaagaactc
     6721 gagcatgggc gcgagggaga gcgggtgcgt tcgctcgctc tcggtcagcg gggttgtgta
     6781 gagatccgcg ctcagcgtgc tcttgtcgac gacgccgccg aatggcgcgg gctgatcgaa
     6841 ggtaggtttc gcggcagggt caagctcacc ctcgggttcc ggatcggcga tgatgaagtt
     6901 ggcatgataa gccgcatagt ccaacgctgc tcgcgtggga gtctgcacct ctcgcaggag
     6961 cacgacggca ttcgtgcgct gatagtcttc gttcagcgtg cgcacctcgg cgtgctcgaa
     7021 cggcgcgttt ctgctcgggg ccgtccacgg atagcgcgcg gcgggatggc aactcacgag
     7081 cagagtggtg aggacgacag cgcgtttgga ttgcagggtc atggctcggg gatggtctct
     7141 tcttgcaagc attcaggccc gctggggggc atcggtcgcg agcgcggaac cccggtttga
     7201 cgcgggtctg ggcccgtaga cgccgcgctg ataacgccac agtacgcgca agaccctgag
     7261 gccatacgaa acgggattca gatatgatat ttcgtcacca tagtgcgtgg gtacgggcgc
     7321 ttcggccacg gccaacccat gcgcgcgcgc gctggcgatc acctcgagat cgaagtcgaa
     7381 gctcttggac aactgcccga acgacagcac ttcgagcgcg cggcgcgaat agaggagata
     7441 gccgctgtga tagtccgtca tgtcgagccc gaacgtcagg ttctccagtt gattcagcaa
     7501 agcattgcca atgtacttgt agagcggcat acccccggac aacgcggtgc cggcggcgat
     7561 ccgcgagccc tgcaggatat ccaggcggcg cgccgtcaaa gcttcgagca gcatcggtaa
     7621 cacttccggg ctgtactgcc cgtcagcgtg cacacacgcg acgacgtcgg cttggctaat
     7681 ccgcgcgtgc tccagaccat cctgcatagc agcgccgtaa ccgccgtttt gcgcgcggtg
     7741 aatcacctgc aggggggcta ggtccgtgcg cctgatcaaa tcatcgagga ggccaagcgt
     7801 cccgtctcga cttccgtcat tgacgacgat aatggtcttc gtgcacgaca gcggcacacg
     7861 cgcgatgcga tcgagcacac cctcgagttt cctctccgcg ttgtaggccg gaatgacgat
     7921 cgcgaggtgc atgtcaaact gctttcaagg cgaactttgc cgggttggcg gggcggatcg
     7981 tattctcgcc gtaactcgat gtctggtcat agctcgatgt ctggccatag cggcgcacgt
     8041 agtcacgcac gatgccgggc aacatctcgg ccaaactggt gcatggttcg aagccgagta
     8101 agcgccgggc cttgctgatg tccggcactc tgactggcgt gtcgtcgtag cccgcgccgt
     8161 agaactccgc tgcggaaaca tcgcgcagcc gagctctggg ctggccggta tacccagcat
     8221 actcgtccgc aagcgcgcgt gcgagctcac gaatcgtgac gtcgttttgg ggattgccga
     8281 gattgataat ctcctcgcgg caagcttcgc cgcgctccac gatgcgcagc acgcccgcgc
     8341 agaactcgtc caccgcgatg aacgagcggc gctgttgtcc gccgtcgacc agctgcagat
     8401 cttctccgcg cagtagcgca gccatgaaac acgcgagcac ccgcggaacc ccgtcgccgt
     8461 cgacgccggg catgtagtcc atgcgcggcc caatcacatt gaatgggcgc acgatggtgt
     8521 agtttaggtt tccgtgcgcg ccgtgcgcgt aaatcacgcg ttcgagtagc tgtttggcac
     8581 aagcgtaagt ccaacgctcg ttgtgcaccg ggcccaagaa caaggcgttg gcctcctcat
     8641 tcatggtctc gcgcggtagc cccgtcagat cgaggctgcg acggccgtag acttcgcacg
     8701 tcgagaagtg aatcaagcgt gcaccgtggc gcgccgaggc acgaaccagt ggtaggagct
     8761 cggtatagtt cgcatcgatc accgccagcg gatccgtgtt gtagagggca ggcgtgcaca
     8821 acgcggtgag cgagagtatg acgctcgatt gcgccgtcac ctcttcgatc aaaccaggct
     8881 gctcgaggcg tgcgactcgc cgctttacgc gcggatcgcg gcactcgagc ttgtgtaggt
     8941 cctgatcgat ggcgtagacc tggacgtctc ccagctccaa caagcggggc acgaggtgag
     9001 aacccagaaa cccgccggct ccgagcacgg cgatgcgtgc cgccgccatt accggcctcc
     9061 ctcgtcatcc aggtgaatgt cgggccagcg gtcattgccc gagtggaacg tgagccgcac
     9121 ggcgccatcc gccggcgtgc cgacctccca caggtaaatc tcgtagtctg caatgtcgtg
     9181 gtcatgtccg cgctgagtcg cgccccacgt catccacttc ccatctcgcg acaggcgcgg
     9241 aaagtactcg tgtgaccggc gcccgggcag gtccatgaac ttgagctgat catcggagag
     9301 atccttgctc gccgcaccgt tggttacggg catcatgaag acttcgctgc caccattgcc
     9361 ggtggggttg acccagtaga tactcgcgtt gtcgggcgtg aaggacacct gacagccgag
     9421 gcctgtcttg tgccaacgct tgccttgaat gtcccaaatg ccagtctccc gttgcgaacc
     9481 gcgcagcgta atcgtgaggt actttccgtc
//
LOCUS       DASTKU010000358         7354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_960164, whole genome shotgun sequence.
ACCESSION   DASTKU010000358 DASTKU010000000
VERSION     DASTKU010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960164"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="VFQ61_12355"
     CDS             complement(<1..1318)
                     /locus_tag="VFQ61_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9955294.1"
                     /translation="MDPLVDFSLPVRSWFKKSFAAPTRAQELGFGPIHAGRSTLLLAP
                     TGSGKTLAAFLSSIDRLMTQPPTEGVRVLYVSPLKALAVDVERNLRAPIAGIQAAAAE
                     LGASVRSPDVAMRSGDTPVQDRARFQRHGGDLLITTPESLYLLLTSQAARHFAQLETV
                     IIDEIHALLPTKRGAHLFLSLERLEALRRSDRPLQRIGLSATQRPLDEAARLLAGGRV
                     TQSADEFVMREIEVVDARMPPRLQVAVRASARSGESASAGAEGEAAEPQKPARSAWPE
                     IHAPLLELIREHRSTLVFVNSRRLAERLAQSLNELEGTEIALAHHGSLAKDRRAFIED
                     QLKLGKLRALVATSSLELGIDMGAIDLVVQVEAPPSVASGLQRIGRAGHSVGALSKGV
                     VFPKHRGELPAAAVVVQGMQEGTVEATRYPRNPLDVLAQQIVAMVSV"
     gene            1443..2573
                     /locus_tag="VFQ61_12360"
     CDS             1443..2573
                     /locus_tag="VFQ61_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955295.1"
                     /translation="MLWQRALRFALWLVVCFCLVAARAEAQASSAFPAREPSALDSEI
                     SAAPARTPAAPAQLDCAELFGGKVCVTRSEAPECPSAAVIAARVLELGTTAVKLPLWV
                     EVEMGRTPGGALRARLRSTGFKAGERELAITSGECATLLDAVSLSLSVLLNLVPASKP
                     AANRGTANTTVPAPEEPTLPAHPEAHQASEQRARDATDRSHEARRAPPLHGFLAAGLG
                     LGSSVLDTPLSAVGSLRAGLLRGRAELGLEVFMLSKQTTAVDPGTIQVNLWGGAAMGC
                     ALFSLSRRVAWSPCVGLSLGRLGADAERLVASRATVRPYWAIRAGAAARFRVSRFGQF
                     ELRALLVAPLSHDSFSVGGHGEAYRTPSWGGILEAGPKLLIW"
     gene            2640..3269
                     /locus_tag="VFQ61_12365"
     CDS             2640..3269
                     /locus_tag="VFQ61_12365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9955296.1"
                     /translation="MSSFEHIYEAHFEFVWRTLRRLGVRPADLPDATQDVFVVVHRRL
                     PEFDGHAKVTTWLFRIAYRTARDRRRRAHVLHEHLDSTVVDTQLDEAPSASHRIELAD
                     DLALFDAALEPMPLEQRAVFTLFELEEQTGPEIAELLEVPLATVYSRLRLAREAFRRN
                     VLRFSAAPRARAGGGRDDQRVDHTERSGAAREPNADAQAAAKLKVEQNP"
     gene            3266..4186
                     /locus_tag="VFQ61_12370"
     CDS             3266..4186
                     /locus_tag="VFQ61_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955297.1"
                     /translation="MTEPKRWLNEPSALSPAERRALEAARRDRPNAAHKRAVWAAIAA
                     GLPVRAGLAKAVAGKTLAATAAPATPVSAVGIVPVLIKPLLLGFALAGMTVGGVRLIA
                     PARTPVPAALRSKDAAPRHGSNSTLGADRATTRPVTPPETTPPETTPPETTPPETTPA
                     AMHVDAPRLGNVGPGENPTRGVGSARRDGSRIKGDQVSPGARELDRNLAAARNDTEPS
                     VFALQEESHRLAEARRTLKSGQLQSAMHQLSAIESDYPNGILNEEREVLILETLSRLG
                     KRQAARQRAERFIERFPKSPHLRAAQSFLE"
     gene            complement(4199..5116)
                     /locus_tag="VFQ61_12375"
     CDS             complement(4199..5116)
                     /locus_tag="VFQ61_12375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET9955298.1"
                     /translation="MFGSSYIQRSVETSRLARSAARIFSEKLAESASLTRSGPRRLWN
                     VGMLLSVEVQRRLLAKSALNHPLLMDMNGSSLSIFVGGGSDEEVFREVFVDDEYGLVD
                     VTPQVIFDVGANVGYSALYFARKYPQARIFSFEPDPRNFRILQRNTASLSRITCFPMA
                     LASHDGEASFYVNRAQGMSSSLVNRAGFDRICVATKTLASVMRQTGVDFVDLLKFDIE
                     GGELALFRKQTELDRVGALIGEVHEDLMGCSLEQFLPLLSGFELNVRRLSACRALVSG
                     KRANGAGARLGAHDHRSFEPAGLRTTRVA"
     gene            5577..5927
                     /locus_tag="VFQ61_12380"
     CDS             5577..5927
                     /locus_tag="VFQ61_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955299.1"
                     /translation="MAAALGYFAGARGLIALGRANEPLASTPLARVGLALQQPGLQTS
                     EKTWAVLRSDVEAVRATLQPEERSVFDLIVAVRGLTNSGDSDWVRAEALCKGLQWSRC
                     DRAALEDLKRSSRP"
     gene            5924..6403
                     /locus_tag="VFQ61_12385"
     CDS             5924..6403
                     /locus_tag="VFQ61_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955300.1"
                     /translation="MKPAARRLVEWLAGPALLAIFAGILSATHRAPPPNEFGRRLASL
                     SEAQRRALEAAPMRAIAEVVWAFGNITVLKRNLESELTRTDDSEPQVRARLLLRRGII
                     EPNPEAQWALFAEACSADSTTCPQIKDLATREAEKRHVSPGSSLPLSLMGSAHPPVP"
     gene            6494..6808
                     /locus_tag="VFQ61_12390"
     CDS             6494..6808
                     /locus_tag="VFQ61_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955301.1"
                     /translation="MDINLAYRTYVGGTVPSAQKLRAQSHDIPPATLEAWISEFEKLH
                     RALEGYLEAGQVSDWKAETQDKVLAFARESHPWLDADSGRSLLSFVGWYGWHEGLIHL
                     GC"
     gene            7167..>7354
                     /locus_tag="VFQ61_12395"
     CDS             7167..>7354
                     /locus_tag="VFQ61_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955302.1"
                     /translation="MASVTIDPKSIRAFENDEAFEAWLSRNHDQKTELYLRIFKKDSG
                     KPTVTYAEALDVALCWGW"
BASE COUNT         1310 a         2412 c         2298 g         1334 t
ORIGIN      
        1 cgaccgacac catagccacg atctgctggg ccagcacatc gagcggattt cgaggatagc
       61 gggtcgcttc gaccgtgccc tcttgcatgc cttgcacgac caccgccgcg gctggcaatt
      121 cgccgcggtg cttggggaag actacgcctt tggagagcgc gcctacgctg tggcccgctc
      181 gcccgatgcg ttgcagcccc gacgcgaccg aaggtggcgc ctccacctgc accaccaaat
      241 cgattgcgcc catgtcgatg ccgagctcga gtgaagacgt cgcgaccagc gcgcgcagct
      301 tgccgagctt gagctggtcc tcgatgaagg cgcgtcgatc tttcgccagg gagccatggt
      361 gagcgagcgc gatttcggta ccctcgagct cattcaggct ttgcgccaac cgctccgcca
      421 agcgtcgact gttcacgaac accagcgtgg agcggtgctc ccgaatcaac tcgagcaatg
      481 gggcgtgaat ctccggccaa gcggagcgag ccggtttctg cggctcagcg gcttcgccct
      541 cggccccagc gctcgcactc tcacccgagc gcgcgctggc ccgaaccgcg acttgtaagc
      601 ggggcggcat ccgcgcgtcc accacctcta tctcccgcat cacgaactca tccgcgctct
      661 gcgtaacacg gcctccagca agcagccgcg ccgcttcgtc cagcggccgc tgcgtcgcgg
      721 agagcccaat gcgctgcaag ggccgatcgc tgcgtcgtaa tgcctccagc cgctccaagg
      781 acaagaacaa gtgagctccg cgtttggtcg gcagcagcgc gtggatctcg tcgatgatga
      841 cggtctcgag ctgcgcaaaa tgccgcgcgg cttgggaggt caaaagcagg tacagggact
      901 cgggggtggt gatcagcaaa tcaccgccgt ggcgttgaaa gcgcgcgcga tcctggacgg
      961 gagtatcacc ggagcgcatc gcgacgtccg gcgaacgaac gctcgctcca agctcggccg
     1021 ccgccgcttg aatgccggcg atgggcgcgc gcagattgcg ctcgacgtcc actgcaagtg
     1081 ccttcagagg cgagacgtac aagactcgaa cgccctccgt cggcggctgc gtcatcagcc
     1141 ggtcgatgct ggagaggaac gcggcgagcg tcttgccgga accggtgggc gctagcagca
     1201 gagtcgaccg ccccgcgtgg atcggcccaa acccgagttc ttgggcgcgg gtcggcgccg
     1261 cgaagctctt cttgaaccaa cttcggaccg gcaaagaaaa gtcgacgaga gggtccactg
     1321 cctggccagc atagtaccgc ttacctgcgc gtcgactggt ggagcctgcg cacgcctccg
     1381 aggctcacga ggcatcgcat gcgtgagatc acgccagcga tgatggcctc gtgcagcgcc
     1441 gcatgttatg gcagagagcc ttgaggttcg cgctgtggct cgtggtgtgc ttctgcctcg
     1501 tagcggctcg agccgaggca caggccagtt cggcctttcc cgctcgtgag ccgtcggcgt
     1561 tggattccga gatctccgcg gcacccgccc gaaccccagc cgcgcccgcc caactcgatt
     1621 gtgcggagct gttcggcggc aaagtatgcg tcacgagaag tgaggctccg gagtgtccat
     1681 ccgccgccgt gatcgcggct cgggtgctcg aactcggaac caccgcggtg aagctcccgt
     1741 tatgggtaga ggtcgagatg gggcgcaccc cgggcggcgc gttgcgcgcc cggctgcgtt
     1801 cgacaggctt caaagctggc gaacgcgagc tcgcgatcac gagcggtgaa tgcgcaacgc
     1861 tcctcgatgc cgtctcgctt tccctctcgg tgttactcaa tctggttccc gcttccaagc
     1921 cggccgcgaa ccgcgggacg gcgaacacca ctgtgcctgc gcccgaagag cccacactcc
     1981 cggctcaccc agaagcccat caggcttctg agcaacgcgc gagagacgca accgatcgca
     2041 gccatgaggc gaggcgcgct ccgcccctcc atggcttcct tgccgcgggc cttgggctcg
     2101 ggtcgtctgt tttggatacc cctctgagcg cggtcggatc cttgagggcg ggcttgctgc
     2161 gagggcgcgc cgaactgggg ctcgaggttt tcatgctctc gaagcagacc accgcggtcg
     2221 atcccggcac gattcaggtc aacttatggg gcggcgcggc catgggttgt gctctgttca
     2281 gcctctcgcg ccgggtcgcc tggagccctt gcgtgggcct ctccttgggt cggttgggcg
     2341 cggacgccga gcgtttggtc gcctcgagag ccacggttcg cccgtactgg gcgatccgcg
     2401 ccggcgccgc ggctcgtttc cgggtgagca gattcgggca attcgagctg cgcgccctgc
     2461 tcgtggctcc gctctctcat gactccttct cggtcggcgg gcatggggag gcctatcgca
     2521 ccccttcctg gggaggcatt ctggaggccg gaccgaaatt gctgatttgg tgatggaacc
     2581 cgcgtccagc tcccctcaac tcgaggaagt ggttcgttcc gcctgcagtt cgcctccgaa
     2641 tgtctagttt cgagcacatc tacgaagctc actttgagtt cgtgtggcgt acgctgcgcc
     2701 ggctcggggt gcgacccgcg gatttgcccg acgccacgca agacgtcttc gtcgtggtgc
     2761 atcgtcggct gccggagttc gatggccacg ccaaggtgac gacctggttg tttcgcattg
     2821 cctatcgcac tgcacgcgat cgtcgacgtc gcgctcacgt gctccacgag cacttggact
     2881 ccaccgtcgt ggacacccaa ttggatgaag ctccctccgc gagtcatcgc atcgagctcg
     2941 ccgatgactt ggctctgttc gacgccgcgc tcgagcccat gccgctcgaa cagcgcgcgg
     3001 ttttcacgct gttcgagctc gaggaacaga ccggtcccga aatcgcggag ctactcgagg
     3061 tcccactggc gaccgtctat tcgcggctac ggctcgcacg agaagctttt cgtcgcaacg
     3121 tacttcggtt tagcgcagcc ccccgagcgc gtgcgggtgg aggccgtgac gaccagcgcg
     3181 tcgaccatac agagcgcagc ggagccgcac gggaacccaa tgccgacgcg caagcggcgg
     3241 ccaagttgaa agtggagcag aacccatgac tgagcccaaa cgttggctca acgagccctc
     3301 cgccttgagc cccgctgagc gtcgggcgct cgaggccgcc cgtcgagacc ggcccaatgc
     3361 cgctcataag cgcgcggttt gggccgctat tgcggccggg ttaccagtgc gagctggtct
     3421 ggccaaagcg gtggccggca agacgctggc ggccactgcg gcaccggcga cccctgtatc
     3481 ggccgttggg atcgtgccgg tgctgatcaa accactgctt ttgggcttcg ctctggcggg
     3541 tatgacggtg gggggagtga ggctgattgc gccggcgcgc accccagtgc ctgccgcgct
     3601 gaggtccaaa gacgccgcgc cgcggcacgg ctcgaattcg acgcttggtg cggaccgagc
     3661 cacgactcgg ccggtcacgc ctcccgaaac cacgcctccc gaaactacgc ctcccgaaac
     3721 tacgcctccc gaaaccacgc ccgccgcgat gcacgtcgac gctcctcggc tgggcaatgt
     3781 cggtcccggt gagaacccga cgcggggcgt tggttctgcg cgccgcgacg gttcccgaat
     3841 caagggtgat caggttagcc cgggagctcg ggagctcgac cgaaatctag ccgcggcgcg
     3901 caacgataca gagccgtcgg tcttcgcgct tcaggaagaa agccacaggt tggccgaagc
     3961 acgacgaact ttgaagtctg gccaactcca gtccgcgatg caccaactga gcgctatcga
     4021 gagcgactat ccgaatggca ttttgaacga agaacgagaa gtcctgatcc tggagactct
     4081 atctcgcttg gggaaaagac aggccgcgcg ccagcgagcg gaacgtttca tcgagcgctt
     4141 ccccaagagc ccccatcttc gagcggcgca gagcttcttg gagtagcggc ttggtcgctc
     4201 aagcaactcg cgttgttcgc agccctgccg gctcgaacga tcgatgatcg tgtgccccga
     4261 gccgggcccc cgcgccgttg gcacgcttgc ccgaaaccag agcacggcag gcggagagcc
     4321 gacgcacgtt gagctcgaaa ccgctcagca aaggcaagaa ctgctccagc gagcagccca
     4381 tcagatcctc gtgaacttct ccaatcagcg caccgacccg atcgagctcg gtctgcttcc
     4441 ggaagagcgc tagctcccct ccttcgatat cgaatttgag cagatccacg aaatcgactc
     4501 cggtctgccg catcacactg gcgagcgtct tggttgccac gcaaatgcgg tcgaagccgg
     4561 cgcggttgac caaagaactc gacattccct gcgcgcggtt cacgtagaag ctagcctcac
     4621 catcgtggct tgcgagcgcc atggggaagc acgtgattct cgacaggctc gcggtgttac
     4681 gctgcagaat gcgaaagttc cttggatctg gctcgaacga gaagatcctc gcctgtgggt
     4741 acttgcgggc gaagtacaaa gcggaataac cgacgttcgc gcccacatcg aagatgacct
     4801 gaggtgttac gtcgactaat ccgtactcgt catccacgaa cacctcgcga aagacctctt
     4861 catccgagcc gccaccgacg aagatgctca atgaactgcc gttcatgtcc atgagcaagg
     4921 gatgattcag cgcagatttt gccagcaacc gcctctgtac ttccactgac agcaacatgc
     4981 ccacgttcca gaggcggcgc gggccagagc gcgtgagtga cgcagattcg gctagcttct
     5041 cggagaagat ccgtgccgcg gagcgagcaa gccgtgaagt ttcgacactt ctctgtatgt
     5101 acgatgagcc gaacatggcc ataagccctt cccttggaaa attgactgtc tccgactaga
     5161 acgagccgag ttgcgcgttc gtgttcggcg tcgttcgtcc tgccgcgcga ggcctactga
     5221 agccttgcgc tcgatcccca atgtccccgc ctatcgatat caataggcct tctcggatcg
     5281 aatttgaagt gcttggagca tctcatcggg attcaatgtc tttttacatg caatgccgga
     5341 ggccgcggca tgactcgcag tcgctggcga ggccgtaccc cttgatgctg gttgcggtta
     5401 gccctcccgg tggtgagcct gcactgttac cgcggctgtg caagtgcgtt acccatctgc
     5461 agcaggaatt cgcgcgctgc gaaattacgt gcccgagtga cgcaaccggt gtttaatgtc
     5521 tcgctcggaa ccgggctttg gctatgcgct tggcacttct gatcggaact tacgttatgg
     5581 cagcagcgct cggttacttc gccggcgccc gtggtctcat tgcgttaggg cgagcgaatg
     5641 aacccctcgc gtctacgccg ctcgcccgcg tgggcctcgc gctccagcag ccggggctcc
     5701 agaccagcga gaagacttgg gcggtcctgc gcagcgatgt cgaggccgtg cgggctacgc
     5761 tacaaccaga agaacgcagc gtattcgatc tgatcgtggc ggtccgtggg ctcaccaact
     5821 cgggcgacag tgactgggtg agagccgaag cgctgtgcaa aggcttgcaa tggtctcgct
     5881 gcgatcgcgc cgcgctcgaa gacctaaagc ggagcagtcg cccttgaagc ccgccgcgcg
     5941 gcgcctggtc gagtggctcg cagggcctgc gcttttggcg atcttcgccg gaattttgag
     6001 cgcgacccat cgcgcacctc cgcccaatga attcgggcga aggctcgcct cactctccga
     6061 agcacagcgc agagcactcg aggccgcgcc catgcgggcc atcgcggaag tggtctgggc
     6121 tttcggcaac atcactgtgc tcaagcgcaa cctcgagtcc gagctgactc gaaccgacga
     6181 ctccgagcct caggtccgtg cgcggctcct gttgcgtcgc ggcatcatcg agcccaaccc
     6241 cgaagcgcag tgggcgctat tcgccgaagc ctgctctgcg gattcgacca cgtgcccgca
     6301 aatcaaagac ctcgccaccc gcgaggccga gaagcgtcat gtctcacccg gcagttcgct
     6361 cccactttcc ttgatggggt cagcgcatcc gcccgttccg tgaccaaccg cgtcgagtta
     6421 gggagcgcgg tcgcagccgc gccgagttag gagcgcggtc gaagccgcgc cgcggtgcta
     6481 tgcccggtgc tggatggaca tcaatctggc ttatcgcacc tacgtcggcg gcaccgttcc
     6541 aagcgctcag aaacttcgcg cgcagtccca cgacatccct ccggcaactc tcgaagcttg
     6601 gatcagtgag ttcgagaaac tgcaccgcgc actcgagggc tatctcgaag ccggccaggt
     6661 ctcggactgg aaggccgaga ctcaagacaa ggtgctcgcg ttcgcacgtg aatcccaccc
     6721 ttggctcgat gccgactctg ggcgctcgct gctcagcttc gtcggctggt acggctggca
     6781 cgagggactc atccatctcg gatgctagcg ctccgaattt gggggtggcc tccaaacaac
     6841 gagcgcgcta cccggcgcgt ggctccggag cgcagctgga gtaagcgctg agttgccccc
     6901 ggcaccgcgc ccgcccgtcc cagtggacgc tacctttggt gcgcaaatgt ccttggcacc
     6961 cgccctcgaa gcctcgcgtc gcgccgcgat tcggagtggc gctgttgctc gaagagcccg
     7021 cagccaaggc cccgcaatga gctccgcgcc gggtccggag tgcggtccgg gccaaaaaca
     7081 cctacgagaa aagccaaatc gggccaattc cggggcgtga agatgcgtgg tggcgctcga
     7141 gcgcgctgcc gtctatgctc ctcttcgtgg ccagcgtcac tattgatccg aagtccatcc
     7201 gcgcattcga aaacgacgag gcgttcgagg catggctgtc tcgcaatcac gaccagaaga
     7261 ccgagcttta tctgcgcatc ttcaagaagg acagcgggaa gcctacggtc acctatgcgg
     7321 aggcgttaga cgtcgccctg tgctggggct ggat
//
LOCUS       DASTKU010000359         2716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1629953, whole genome shotgun sequence.
ACCESSION   DASTKU010000359 DASTKU010000000
VERSION     DASTKU010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629953"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1962
                     /locus_tag="VFQ61_12400"
     CDS             <1..1962
                     /locus_tag="VFQ61_12400"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9955303.1"
                     /translation="GWLRALNLPSHVLQDFLPAPLESARFEQRLYALPWFVDVGMLYY
                     RKDWFATAPATFSELFERARQATDQFGAREGFVFTGARYEGLTTVFVEVLGGFGGSIL
                     TPDSRASALDSPAALGALSWLTRAVQDSRVSPRTVLSSQEEQVRFEFQSGRAAFMRNW
                     PYAYALMQRDPSSAVRGKVGIAPMPHEVGGSSTAALGGQQLAINVHSRSPELAQSFIE
                     FACRPEQMLERAEIAAELPARRSVYEDPRLPAALAFPVNEILPVVERATARPTSPAYS
                     ELSSELQIQLHAALTQQLSPRAALRVAHERVTRVIRATGSPAAARSQANHPSQRWPAW
                     LWLVLGSLVTGGILGVLGRRPRSSLGGAQQAREQRLGSLLIAPTLLCVVLLTLVPLWS
                     AIADSLHAHDLRKPWLGRPFVGLANYSEVFGAGRFWGALVRTTLFTLVSVSLELVAGL
                     AGALLLARTFRGRGLMRALALLPWALPTVVVALTFRFLFDSRSGPVHLLVSGLGLIGR
                     ERDWFSDELLAWIPILLADVWKTSPFVTLLLLSGLSSIPPSVYEAAALDGATRMRQLW
                     YFTLPMLRQLIYVVVLLRALDAFRVFDLIYVLTQGGPGIATEPLAIYTHQVLLRNLDF
                     GHGAALSVVIFAFTLALCALALLLMRRKA"
     gene            1978..>2716
                     /locus_tag="VFQ61_12405"
     CDS             1978..>2716
                     /locus_tag="VFQ61_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9955304.1"
                     /translation="MTKPAAVRRTGGELASTRRPLLALARAAAAILLLCPFAFVCLAS
                     VLPEQSLFGGAKPGGSAGLFHYRELFRARGFATPLRNSLYVAGGTTALCVGLGALAAY
                     ALARLEFRGKRGVFGLLLAVSAFPQISVVEPLYALLRELGLINTYPGLVLAYVTFTLP
                     ISVLLLVSFFRELPREVEQAARMDGATSLQVLWHVILPLSVPGLVATGIISFVYCWNE
                     FLFALSFTVGGERQTLPVAIAMLRGRHQ"
BASE COUNT          393 a          872 c          862 g          589 t
ORIGIN      
        1 ggttggttgc gagctttgaa tttgccttcc cacgtgctgc aggacttcct gccggcacca
       61 ttggagagcg cacgtttcga gcaacggctg tatgcgctgc cgtggttcgt cgatgtcggg
      121 atgctctact atcgtaagga ctggttcgcg accgccccgg ccacattttc ggagttgttc
      181 gagcgcgcac gacaggcgac cgatcagttc ggcgctcgcg aaggctttgt ctttacgggt
      241 gcgcgatatg aggggcttac gaccgtgttc gtggaggttc tgggcggctt cggcggttcc
      301 attctcacgc cggatagcag ggcttcggcc ctcgactcac cggccgcgct gggggcgctg
      361 agctggttga cccgcgccgt tcaggactcg cgagtctcac cgcgcacggt gctcagctcc
      421 caagaggaac aagttcgctt cgagttccag agcggacgcg cggcgttcat gcgcaattgg
      481 ccgtacgcgt acgcgctcat gcagcgcgac ccgagctctg cggtgcgcgg caaagtcgga
      541 atcgcgccca tgcctcacga agtcgggggc agctccaccg cggcgcttgg cggtcaacaa
      601 ctggccatca acgtgcattc gcgctctcct gaattggcgc agagcttcat cgagtttgcc
      661 tgccggcccg agcaaatgct cgaacgcgcc gagattgccg cggagctgcc ggcgcgacgc
      721 tcggtgtacg aggatccgcg gctgcccgcc gcgttggcct ttccagtaaa cgaaattctg
      781 cctgtagtcg agcgagcaac ggcacgacct accagccccg cgtatagcga gctctcctct
      841 gaactacaga tccaactgca cgccgctctc acccaacagc tctcgccgcg cgccgcgctg
      901 cgcgtagcgc atgagcgcgt gacccgcgtg atccgagcga cgggaagccc cgcggcggct
      961 cgctctcagg caaaccatcc ttcgcagcgc tggcccgctt ggctctggct ggtgctcggt
     1021 tccttggtga cgggcgggat cctcggcgtg ctcggccggc gccctcgttc atcgctcggg
     1081 ggcgcacagc aagctcgtga gcagcgactc ggcagcctgc tgatcgcgcc caccctgctg
     1141 tgtgtagtgc tgctcacgct cgtcccgctc tggtccgcca tcgccgattc gctccatgcc
     1201 catgatctgc gaaagccgtg gctcggccgc cctttcgtgg gactcgcgaa ctactccgag
     1261 gtgttcgggg ctggccgctt ctggggggct ctcgtgcgca cgaccctctt tacattggtg
     1321 agcgtgagtt tggagttggt agccgggctg gctggagcat tgctcttggc gcgcacgttc
     1381 cggggtcgcg ggctgatgcg agcgctggcc ttactaccct gggcactgcc gaccgtcgtg
     1441 gtcgcgctca cgttccgctt cctgttcgat tcgcgctcgg ggccggtgca cttgttggtg
     1501 agcgggttgg gtctgatcgg cagagagaga gactggtttt ccgacgaact gctggcatgg
     1561 attccgatcc tgctcgccga tgtttggaag acctcaccct tcgtgacgct gctcctactg
     1621 tccggtctct cgagcattcc gccatcagtt tacgaagcag cggccctaga cggagcaact
     1681 cgtatgcgac agctctggta cttcacccta ccgatgttgc gacagctgat ctacgtagtg
     1741 gtattgctgc gggcgctgga tgcctttcga gtcttcgacc tgatttacgt gctcacccaa
     1801 ggtggtcccg gcatcgccac cgaaccgctc gcgatctaca cccaccaagt cttgcttcga
     1861 aacctggact tcggccacgg cgccgcgctg tcggtcgtaa ttttcgcgtt cacactggcg
     1921 ctgtgtgcct tggcactgct gttgatgcga cgcaaggcgt agccgagagc tggagctatg
     1981 accaagccag ccgccgtgag aaggactgga ggcgagctcg cgagcacgag acgtccccta
     2041 ttggcgctcg ctcgcgctgc ggcggcgatc ctgctgctct gcccctttgc cttcgtatgt
     2101 ttggcttctg tactacccga acagtctcta ttcggcggcg cgaagccggg cggttcggcg
     2161 ggactgtttc actaccgtga gttgtttcgg gcccgcggat ttgcaacgcc gctccgaaac
     2221 tctttgtacg ttgcgggcgg cacgaccgcg ctgtgcgttg gcctgggagc gctcgctgct
     2281 tacgccctcg cacgcctgga atttcggggc aaacgcgggg ttttcgggct cctgctcgcg
     2341 gtcagcgcgt tcccgcagat ctccgtcgtg gaaccgctct atgcgctgct gcgcgagctc
     2401 gggttgatca acacttaccc ggggctcgta ctcgcgtacg tcacgttcac gctcccgatc
     2461 tccgttttgt tgctggttag cttcttcagg gagctgcccc gcgaggtgga gcaagccgcg
     2521 cgcatggacg gcgccacgtc gctacaagta ctctggcacg tgatcctacc gctctcggtg
     2581 ccggggctcg tcgcgaccgg catcatcagc ttcgtctatt gttggaacga gttcttgttc
     2641 gcgttgagct tcaccgtggg aggcgagcgg cagactctgc ccgtcgcgat cgcaatgctg
     2701 cgggggcgac atcagg
//
LOCUS       DASTKU010000360         6320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1630045, whole genome shotgun sequence.
ACCESSION   DASTKU010000360 DASTKU010000000
VERSION     DASTKU010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630045"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFQ61_12410"
     CDS             complement(<1..485)
                     /locus_tag="VFQ61_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955305.1"
                     /translation="MRVLVLMAATAGCNSLLGNEEAHRDDATGGRATTTGGRATASGG
                     MGATDPQAEGGAGAGSESSGGRGGASLEPDSGAPNAGSGADGGVSGGSESSEGGASPE
                     LGGSSGNRGGASAGGASHGGTVNVSGGTVNASGGSATNGGTSTGGANSAGATSMGGAV
                     AV"
     gene            complement(790..1761)
                     /locus_tag="VFQ61_12415"
     CDS             complement(790..1761)
                     /locus_tag="VFQ61_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955306.1"
                     /translation="MLHNRTSPWLLAAGAAAAGLVVTGAVLSRRRARSGLFGKPSRSR
                     FESLEAQLDDLSSERDATQSLSDKNAEALRSEQKRYADMPVAAREALEASSRVHGIRS
                     FGGAASGEFSQNIADRTRTRQEQRSHEAGRNPWEDSGAITQQLALDDADIGQALNPSE
                     VPEVPLGVSADPEIENLLTTPGRAQATDSTGASVLDASSDLADSHEQINPATEDLPEI
                     TAQPTRNYAPSDDAYDDVSPEDLGAEYLLRATQGATAPHEADLAALPPELYQEEGLSV
                     VSEESISTAQADVMEEDVAMRMRARKPKPADVRPPSSDPEPNDAERE"
     gene            1933..2388
                     /locus_tag="VFQ61_12420"
     CDS             1933..2388
                     /locus_tag="VFQ61_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955307.1"
                     /translation="MRPRSLVKPLPLPLAAVIALCVSACGGGATSVLHGQGKPTGAGD
                     AELTIKNSSGASVAQLYITKTEVVEQAHKAGVSAGTEADRNLWGADELGNASIGDGST
                     WSALHLPPARYDVLVVATDHREQLVKRLNLQAGGKYVLELRDAWKRGRD"
     gene            2633..4204
                     /locus_tag="VFQ61_12425"
     CDS             2633..4204
                     /locus_tag="VFQ61_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955308.1"
                     /translation="MQSREPGSSLRTVTFLLFVAQVLRSAWLSDDAFYSIRAADNLAH
                     GFGLTFNPGERVQVFTNPLWTLLLSTLLVFFKSAYFVALLAGVVASAGAVLLAATRIA
                     RTASAAAVGILLCCLSRAFVDFSTSGLENSLGFLLLASFFWAYWAGRTWRRQIFWLAL
                     FGGATAFNRLDAILIVAPALAHALFVRRSLRSLGLTLLGFAPLLGWLLIAKLYYGVAL
                     PNTAYAKLNLALPSGQVWTQGLAFFTDSLNRDPITLLTCAWMIVACFRRLGPAQGADD
                     DRNQALFASLGALLYLVYVASVGGDFMSGRSLTLPFFVAVLVFVRCVGPQQSRAELQA
                     VAAIAAATVFVLPHGTMVEHTECVVPDSGVVNERYCYETHTALLYNLHSEFYKTHPYW
                     QEGARAAAENRRIVEAGAGLAAQAAGPRLYVLNGFAMGDALLSRIRFVPNGGWRIGHY
                     FRLNPPGYLESLESGRNELSDPCMRRFYAELRVLIREPLFSWRRIRRVFSASELDSYE
                     TWAASHCSGLDAAQH"
     gene            4366..6231
                     /gene="ilvD"
                     /locus_tag="VFQ61_12430"
     CDS             4366..6231
                     /gene="ilvD"
                     /locus_tag="VFQ61_12430"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET9955309.1"
                     /translation="MPEYRSRTTTHGRNMAGARALWRATGVRDEDFGKPIIAVVNSFT
                     QFVPGHVHLRDLGRMVAEEIERAGGIAREFHTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVETMVSAHCADGMVCISNCDKITPGMLNAAMRLNIPAVFVSGGPMEAGKVQLKDKSV
                     KKLDLIDAMVAAANPKVSDEDSERIERSACPTCGSCSGMFTANSMNCLTEALGLSLPG
                     NGTVLATHSARKDLFLRAGRLVIELVKRYYEQGDASVLPRSIANMKSFENAMALDIAM
                     GGSTNTILHLLAAAKEAGVPYDLSCIDKLSRRVPNLCKVAPSTEKYHIEDVHRAGGVM
                     GILGELDRAGLIHRDTPTVHEKTLGAALDRWDVIKNPEVGTFFRAAPGGVPTQTPFSQ
                     SRTWDELDVDRQQGCIRDAEHAYAKDGGLAVLFGNLAEQGAVVKTAGVDASILRFSGP
                     ARVYESQEDAVDGILGGKVQAGDIVVIRYEGPRGGPGMQEMLYPTSYLKSQGLGKACA
                     LITDGRFSGGTSGLSIGHVSPEAAEGGLIALVRDGDRIEIDIPERRLHLAVSDAELAE
                     RRSQEQARGGAAFTPQNRERVVSTYLRAYAALATSAAQGAVRDVSRLDSLTKTPR"
BASE COUNT         1086 a         2063 c         1995 g         1176 t
ORIGIN      
        1 accgcaaccg cgccccccat gctggtggcg ccagccgagt tggctcctcc agtgctggtg
       61 ccgccgttcg tggcagagcc gccgctagcg ttcacggtgc cgccactaac gttcacagtt
      121 ccgccatggc tagcacctcc cgcggacgca ccgccgcgat ttccggagga gcctcctagt
      181 tcggggctgg ctcctccttc agaggattcg ctgccgccgg agacgccgcc atctgcgccg
      241 ctacccgcgt tgggagcgcc ggagtccggc tctaacgagg ctccgccgcg ccctcctgat
      301 gattccgacc ctgcccccgc cccaccctcg gcttgcggat ctgttgcgcc cattccgccg
      361 ctcgccgtcg cgcgcccacc cgtcgtggta gcgcgcccgc cagtcgcgtc gtcacgatgg
      421 gcttcttcgt tgcccaggag cgagttgcag ccggccgtgg ccgccatcaa gaccagaacg
      481 cgcacgagtt ccgtcgcaga ccgacctcgt cgggggacaa gtccagggaa gcatcgggga
      541 gccacagcgc ggcagagtct acagcgaacc aggctcaaat caacgcaagc ggggactgcg
      601 ctcctcgcga gagcgcagcg gtcatctcgg gcaggcttgg tgatggttgc tccagcccga
      661 ctccaagcgc tgccatgctc ggacgccgat cactcccggc tatgagaacg tcgacggagc
      721 tgacccgcga gccgcgccgc ggattgggcc aaccatcact cgcaagggta atgatcggca
      781 gagacgtgct cattctctct cggcatcgtt gggctcggga tccgagctcg gcggtcgaac
      841 atcagcaggc ttgggtttcc gcgcccgcat tcgcatcgcc acgtcttctt ccatcacgtc
      901 cgcctgagcg gtcgagatgg actcctcact cacgaccgag agcccctcct cctgatacag
      961 ttcagggggc aacgccgcca gatccgcctc atgaggtgcg gtggctccct gcgtcgcgcg
     1021 cagcaaatac tctgcgccca agtcttcggg tgaaacgtcg tcgtaggcgt cgtcagaggg
     1081 cgcatagtta cgcgtcggct gagccgtgat ttccggcagg tcttcggtcg ccggattgat
     1141 ctgctcatgt gaatcggcca agtcgctgct cgcatcgagc acgctcgcgc ccgtggagtc
     1201 ggtcgcctgc gcacgcccgg gggtcgtgag taggttctca atctcagggt cagccgagac
     1261 gccgagcgga acttcgggaa cctctgaagg attgagcgct tgcccgatat cggcatcgtc
     1321 gagcgccaat tgttgcgtga tcgctccgga atcctcccag gggttccttc ctgcctcgtg
     1381 gctacgctgc tcttggcgcg ttcgtgttcg atccgcaatg ttctgcgaga attcaccgga
     1441 cgccgccccg ccaaacgagc gaatcccgtg aactcgggaa gaagcctcca gcgcttctcg
     1501 cgctgcgacc ggcatgtcgg cgtagcgttt ttgctccgag cgcaaagctt cggcgttctt
     1561 atccgacagc gattgagtgg cgtcgcgctc gctcgaaaga tcgtccaatt gcgcctccaa
     1621 agactcgaaa cgcgatcggg acggcttccc gaagaggcca cttctggcgc ggcgcctgga
     1681 caagacggct cctgtaacga cgagacccgc ggcggcggcg cccgcggcga gcaaccaagg
     1741 actggttctg ttgtgcagca cgattgcgtt cctttcttga cccgaaggcg gtccggtctt
     1801 cctcggtcat cctcccagca acccgcgtgc cgtcgtaggc cctcgtgctg agcgcggccg
     1861 gccccgcgaa acagatcccc agtggcccct cggaactggg agcgggagca gatcggttct
     1921 aagctccaaa caatgcgacc gagaagccta gtcaagccgc tcccgctccc gctcgccgcc
     1981 gtcattgcac tctgtgtgag cgcttgcggc ggtggcgcga catccgttct tcacggtcag
     2041 ggcaaaccga ccggcgcggg cgacgcggag ctcacgatca agaactctag tggagcgagc
     2101 gtcgcgcagc tttacatcac caagaccgag gtcgtcgaac aggcccacaa ggcaggcgtc
     2161 tccgcgggaa ccgaggcgga ccgaaacctg tggggagcgg acgagctggg gaatgcgagc
     2221 attggagacg gcagcacctg gtcggcgttg cacttgccgc ccgcacgtta cgacgtgctc
     2281 gtggtcgcaa ccgatcaccg cgagcaactc gtgaagcgac tgaatctgca agcaggtggc
     2341 aagtacgtgc tcgaactccg cgatgcgtgg aagcgaggtc gggactaggc aaggtcggaa
     2401 gcgtgctggg cgggcgcccg cagagccttg ctctggcggc agggagcgca taccccacgc
     2461 agtgcgagcg ggtgatggta tatggcgggt ccaaatggga ctcgatggga ccgacacagg
     2521 cgaaaggact ccggggaacg agatacccct acagcgctcc ggcggctcga agcatcgctt
     2581 tggcctccag tcgttacatg acgccgtagt gcgtatctgg cgttcccaga acgtgcaatc
     2641 ccgggagccg ggtagttcgc ttcgcacggt cacgttcctg ctgttcgtcg cacaagtgct
     2701 gcggagtgct tggctttctg acgacgcttt ctacagcatt cgagccgccg ataacctggc
     2761 ccacgggttt gggctgacct tcaatcccgg cgagcgggtg caggtcttca ccaatccgct
     2821 ttggacgctg ctgctgtcga cgttgctcgt gttcttcaag agcgcctact tcgtggctct
     2881 actcgcgggc gtggtggcga gtgcgggcgc cgtcctattg gccgcaacac gcatcgcaag
     2941 gaccgcgagc gcagcggcag tgggaattct gctctgctgc ctctcgagag cgttcgtaga
     3001 cttttcgacg agcgggctcg agaactcgct cggattcttg ctgctggcct cgttcttttg
     3061 ggcctactgg gcggggcgaa cctggcgtcg ccaaatcttc tggctcgcgc tattcggcgg
     3121 cgcgacggcc ttcaatcgac tggatgcgat actgatcgtt gcgcctgcgc tcgcccacgc
     3181 cttgttcgtg cgtcgctcac tgcgaagctt gggcctgacc ctactgggtt tcgcaccgct
     3241 actcggctgg ttgcttatcg ccaagctcta ctatggagtc gcgctcccca ataccgccta
     3301 tgcaaagctg aacctggcgc tgccatccgg ccaagtctgg acgcaggggc tcgcgttctt
     3361 cactgactct ctcaatcgag atcccatcac gctgctcacc tgcgcctgga tgatcgtcgc
     3421 gtgcttccgt cgccttgggc ccgcgcaggg tgccgacgac gaccgcaacc aagcgctgtt
     3481 cgcgagcctt ggcgccctac tttatctggt ctacgtagcg tcggtgggtg gcgacttcat
     3541 gagcgggcgt tcattgacgc tgccgttttt cgtggcggtg ctggtgttcg tgcgctgcgt
     3601 cggcccccag caatctcgcg ccgaactcca ggccgtcgcg gcgattgcgg ccgcaaccgt
     3661 gttcgtgctg ccacatggca cgatggtgga acataccgag tgcgtagtgc ccgatagcgg
     3721 cgtcgtgaac gagcgctact gctacgagac gcacaccgcc ctgctctaca atttgcattc
     3781 tgagttctac aagacgcatc cgtactggca ggagggcgcg cgagccgcag ccgagaacag
     3841 gcggattgtg gaggcgggcg ccgggctcgc cgcgcaggcg gcaggcccgc ggctctacgt
     3901 cctgaacggc ttcgccatgg gtgacgccct gctctcgcgc attcgtttcg tgccgaacgg
     3961 aggttggcgc attggccact acttccggct caatccgccc gggtacttgg aatcgctgga
     4021 gtccggccgt aatgaacttt cggacccgtg catgcgccgg ttctacgctg agttgcgcgt
     4081 cctcatccgg gagcccctgt tcagctggcg ccgcatccgg cgcgtctttt cagcctcgga
     4141 gctcgactcc tacgagacct gggccgcgag ccactgttca ggcctcgacg ctgcccagca
     4201 ctagtgcgac ggcgatgacc agcttcacgg ccaaagcgcc agtttcgcag cgccgcgggc
     4261 ctcatagcct gcacctgggc tgcgggaacg catgttgcgg aaaatgcaac gcggtcttgt
     4321 gggagtagcg acagacgcaa gagccgacgg tgctagcctg cgcgcatgcc cgagtaccgc
     4381 tctcgaacca ccacccacgg tcgcaacatg gctggagccc gcgcgctctg gcgtgccacg
     4441 ggggtgcgtg acgaagattt tggcaagccc atcatcgccg tcgtcaattc cttcactcag
     4501 ttcgtccctg gccacgtcca cctgcgcgac ttgggtcgca tggtcgccga ggagattgaa
     4561 cgcgcgggcg gcatagcgcg cgagtttcac accatcgcgg tcgatgacgg catcgccatg
     4621 ggccacgggg gcatgctcta cagcctgcct tcgcgcgagc tgatcgcaga ttccgtggag
     4681 accatggtgt cagcgcactg cgccgacggc atggtgtgta tctcgaactg cgacaagatt
     4741 acgcccggta tgttgaacgc cgccatgcgg ctcaatatcc ctgcggtatt cgtgtccggc
     4801 ggtccgatgg aggccgggaa ggtccagctc aaagacaagt ccgtcaagaa gctcgacctg
     4861 attgacgcca tggtcgcggc ggcaaaccct aaggtcagcg acgaagactc cgagcgtatc
     4921 gagcgctcgg cttgtcccac gtgcggctcc tgctcgggca tgttcacggc caattcgatg
     4981 aactgtttaa cagaggcgct cggtctgtcc ttaccgggta acggcacggt gctggccact
     5041 cactccgcgc gtaaggatct gttcttgcgg gcagggcgcc tcgtcatcga gctcgtaaag
     5101 cgttactacg agcaggggga tgcgagcgta ctgccgcggt ccatcgccaa catgaaatcc
     5161 ttcgagaacg cgatggcgct ggacattgcg atgggcggct cgaccaacac gatcctgcac
     5221 ctgctcgcgg ccgccaaaga agccggcgtg ccctacgatc tctcgtgcat cgacaagcta
     5281 tcgcgacgtg tacccaattt gtgcaaggtc gcgcccagca ccgagaaata tcatatcgaa
     5341 gacgtgcacc gcgcgggcgg cgtcatgggt attctgggcg agctcgatcg agccggcttg
     5401 atccaccgag ataccccgac cgtgcacgag aagacgctcg gggccgccct cgatcgttgg
     5461 gacgtgatca agaaccccga agtgggtacg ttcttccgag cggcaccggg cggagttccg
     5521 acgcaaacgc cgtttagcca aagccgcacc tgggacgagc tcgacgtcga tcgtcagcag
     5581 ggctgcatcc gtgacgccga gcacgcttat gccaaagatg gcggcctcgc cgtgttgttc
     5641 ggtaacttgg cggagcaggg tgcagtcgtt aagacggctg gtgtggacgc ttcgattctg
     5701 cgcttcagcg gccccgctcg cgtctacgag agtcaagaag atgccgtgga cggcatcttg
     5761 ggtggcaagg tgcaagccgg cgacatcgtc gtgattcgct atgaaggccc ccgcggtggg
     5821 ccgggcatgc aagagatgct ctacccgacc agctatttga agagccaggg actcggcaag
     5881 gcgtgcgcac tgattacgga cgggcgcttc tcgggaggca cctccggact ttcgattggg
     5941 cacgtatcgc cggaagccgc cgagggcggc ctgatcgcgc tggttcgaga cggagaccgc
     6001 atcgagatcg acatcccgga gcgtcgtctg cacctcgccg tcagcgacgc tgagctcgcg
     6061 gagcgacgct cgcaagagca agcgcgaggc ggagctgcct tcacgccgca aaaccgcgag
     6121 cgcgtcgtca gcacctatct gcgcgcgtac gccgcgctcg caaccagcgc ggcccaaggc
     6181 gccgtccgcg acgtctcacg cctcgatagc ctcacgaaaa caccgcgcta agctcagtca
     6241 agctccgtca cgtcggagcg cgttcaccgc gatagccctc gatactgggc gccactcatt
     6301 gagccgctcg cgaaccaacg
//
LOCUS       DASTKU010000361         5732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1854034, whole genome shotgun sequence.
ACCESSION   DASTKU010000361 DASTKU010000000
VERSION     DASTKU010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854034"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VFQ61_12435"
     CDS             complement(<1..428)
                     /locus_tag="VFQ61_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955310.1"
                     /translation="MSTARFALFSVLLGVLIWAANRYFRRWLVGAFALSPRWARILDV
                     ALYVALFGSVVRPFRPFTLVAASVELTVLLSLVPLWAIDITRWLLKRAAPPLPTPPAP
                     TTESAAISPAPTESPVPAPTSADPHPEIELPFSRRALLSRA"
     gene            complement(514..936)
                     /locus_tag="VFQ61_12440"
     CDS             complement(514..936)
                     /locus_tag="VFQ61_12440"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HET9955311.1"
                     /translation="MRPVQSSGERGKNTQSETLIFVYGTLRRGEANHALLGASTSRGP
                     GLTRPQFELFDLGEYPGMAPEGTTCVVGEVYAVSPETLRRLDRLERHPTWYERKPIQL
                     SDGLWVEAYVLPRAYCKRGHPIASGDWLTRGTGPTPTR"
     gene            993..1793
                     /locus_tag="VFQ61_12445"
     CDS             993..1793
                     /locus_tag="VFQ61_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955312.1"
                     /translation="MLLPSNMAANDSNTASERPANVSADAAANTENWAAARAELQGLV
                     EPMPSALRKLLTEEWSNAARFEHASIASFSRFSLELMAVGAPPEFLVGAHRAALDEIR
                     HAELSFALASVYAGKALGPAALPMDRVNFGPYELASIVEHTVEEGCVMETLAALEAQA
                     ALERAEPKAVQHVLSVVTRDEQAHATLAFRFVAFARARTGRALDAVIERGFETTMRRV
                     RAESPMVAPLDERLAAHGRLTGEERHALRQRALVGVLDQARGALLASS"
     gene            1925..2842
                     /locus_tag="VFQ61_12450"
     CDS             1925..2842
                     /locus_tag="VFQ61_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955313.1"
                     /translation="MRYVWLGRGLGEWLVRLALLGGSSLALVGGLAGCGGEDGSGPPS
                     AQSGNSNTGASSGSGGKNAGTGGKAQGGQLSSGGASGATSTPPNPSDKGYGEPVSGQY
                     HLGPVDFGETEWHNACAPAGGYRQALRDATGLGGEFLAGLSNEHAGGGSRCDACIAIR
                     TATGRAIVARVVTYGVEAEPGDIDVSPSVYEALNTGEYPRSMTWQFTDCPGSAKLQYE
                     FQTGANVWWTSFWVRNGKVPIEKVEVQSANNQEFLTLRRELDGTLNADSGFGEGTFTL
                     RLTGAGGRVLTDSFPKFEPGQLISSEKQF"
     gene            2923..3342
                     /locus_tag="VFQ61_12455"
     CDS             2923..3342
                     /locus_tag="VFQ61_12455"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HET9955314.1"
                     /translation="MHVRGTFEIKMQAEPPYDDVGGVPLSRASFEKQFSGALQATSQV
                     QMLAARTPIPNSAGYVALERIVGSLEGKQGSFVVVHVGIMTRGERSLQIRIVPDSGTD
                     QLQGIAGRMDIQIIEGQHFYELEYSLGAPAQNQGNAQ"
     gene            3679..4545
                     /locus_tag="VFQ61_12460"
     CDS             3679..4545
                     /locus_tag="VFQ61_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955315.1"
                     /translation="MNIRFRPLTLLGFLFTAQVLVFACGGTPGSGSLTTNSGGTSAIP
                     SPMGGKVSSGGTQGEPTIPSDEGGASSEPTAGATSAGGDSSSGGTSNSGGTTGGVETS
                     GGTTGMSSAGAGNEGGAEGLPEFCDDVTCKIDCTKQGSCPERVECPKGRNCEVTCGDD
                     ACAKGVQCNDETQACTVTCQGRNSCAGEVECAGKKACQVKCNGDDSCKSRIYGGSGER
                     IQVDCNAARSCSGGVLANAPQVAIFCNGDSSCRGDLQCNTDRCEVRCNSQNSCTGSVQ
                     CNAGQKIGACTR"
     gene            complement(4653..5180)
                     /locus_tag="VFQ61_12465"
     CDS             complement(4653..5180)
                     /locus_tag="VFQ61_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955316.1"
                     /translation="MTRYGTALVLCFGLLGPVAAIGVAHADLLPPDACQNEGETCDNA
                     TGGSTSREGVCLTRTCTRVGPPFTNCEGGAAGAAAGGTTATCGPTTTTYDCLRCIASS
                     GGSSSAGAENTAGATHTGGHTATGGTSSAGDSSKDDSSCDCAIGRRTSERGLALAMLA
                     IGAIAWRRARRRLQG"
     gene            5452..5661
                     /locus_tag="VFQ61_12470"
     CDS             5452..5661
                     /locus_tag="VFQ61_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955317.1"
                     /translation="MVGNSSPLSTASPEDFLHRHVAALHRKLHRIGVSASFGLSEAGI
                     EAAFERVTPAGHGRELLSELKSLGS"
BASE COUNT         1057 a         1738 c         1945 g          992 t
ORIGIN      
        1 gctcgcgaga gcaacgcgcg gcgtgaaaac gggagctcga tctcgggatg agggtcggcg
       61 gaggtggggg cagggacagg agattccgtg ggagccggcg agatggccgc gctctccgtc
      121 gttggcgcgg gtggcgtggg cagcggcggg gcggctcgct tcaacaacca gcgcgtgatg
      181 tcgatggccc acagcggcac gagcgacagt aggaccgtga gctccacgct ggccgcgacc
      241 aacgtgaagg gtcggaatgg ccgcaccacc gagccgaaca gcgcgacata gagagccacg
      301 tcgaggattc gtgcccagcg cgggctgagc gcaaaggcgc cgacgagcca acgtcggaag
      361 taccgattgg cggcccaaat caggacgccg agtagaaccg agaacaaggc gaagcgcgcc
      421 gtcgacatgt gcgcccagtc tagctagccc gtggctactt tcacgggctt cgcgggggtc
      481 gaggcgaacg cacgaatgcc cgagacggct ggactaccga gtcggggtcg gccccgtacc
      541 acgtgtgagc cagtcgcccg atgcgatcgg gtggccccgc ttgcagtacg cgcggggcag
      601 aacgtaagcc tccacccaaa ggccatccga gagttgaatc ggcttacgtt cgtaccaagt
      661 cggatggcgc tcgaggcgat cgagccgacg cagcgtctcg gggctcacgg catacacctc
      721 ccccaccacg caggtcgtgc cctcgggcgc cataccaggg tactcaccca ggtcgaagag
      781 ctcgaactgg ggccgggtca ggcccgggcc acggctggtg cttgccccga gtaacgcgtg
      841 gttggcttca ccccggcgta gggtcccgta tacgaagatt agcgtctccg actgcgtatt
      901 cttacctcgt tcacccgagc tctggacagg acgcactcgg cacatattga ccctgcgctt
      961 acacttggcc acgcctcgcg aacgcgcgag ccatgctact accaagcaat atggcggcaa
     1021 acgactcgaa cacggcttcg gagcggcctg caaacgtatc ggcagatgcg gccgcgaaca
     1081 cggagaactg ggcggcggcg cgcgctgagt tgcagggcct ggtggagccg atgcccagcg
     1141 cgcttcgcaa gttgctgacc gaggagtggt cgaacgccgc ccgcttcgag cacgcctcga
     1201 tcgcgtcctt ctcgcgcttc tcgctcgagt tgatggcggt cggggcgccg ccggagttct
     1261 tggtgggggc gcatcgagcc gcgctcgatg aaatccggca tgcggagctg agctttgcgc
     1321 tggcctcggt ctatgcgggc aaggcgttgg gtccggcggc tctgccgatg gatcgggtca
     1381 acttcggtcc ctacgagctg gcgtctatcg tcgagcacac ggtcgaggaa ggctgcgtga
     1441 tggagacgct ggccgcgctc gaggcgcagg cggctttgga acgcgccgaa ccgaaagcgg
     1501 tgcagcatgt gttatcggtc gtgacgcgcg atgagcaggc tcacgccacg ctggcgtttc
     1561 gcttcgtcgc ttttgcccgg gcgcgcaccg ggagagcgct ggacgcagtc atcgagcgtg
     1621 gtttcgagac caccatgcgg cgcgttcgtg cggagtcccc catggtcgcg ccgttggacg
     1681 agcggctcgc cgcccacggt cgcttgacgg gagaagagcg gcacgccttg cggcagaggg
     1741 ccttggtggg tgtgctcgat caggcgcgcg gcgcgctgct cgcgtcttcg taacggaaca
     1801 cgccgctttc gatgactggc ttccgtgtcg cgcacgaagt gcatgtagtg cagtctgcgt
     1861 ggcctgctcg ttgaacgaga gttcagcctt cttcgaactg aagcggtctg ttaggttcaa
     1921 cacgatgagg tacgtgtggc tgggacgcgg tcttggcgag tggctggttc ggttggctct
     1981 gttgggaggc agctcgctcg ctttggtagg cgggctcgcg ggttgcgggg gagaagacgg
     2041 atcgggaccg ccctcggcgc agagcggaaa ctccaacacc ggagccagct cgggatcggg
     2101 cgggaagaac gccggaactg gaggtaaagc ccaaggcggg cagctctcga gcggcggcgc
     2161 gagcggagcc acttcgacgc ccccgaaccc gagcgataag ggctacggtg agccagtgtc
     2221 cggccaatac cacttgggcc cggtcgactt cggcgagacc gagtggcaca acgcctgcgc
     2281 gcccgcggga ggctatcgtc aggcgctgcg ggacgcgacg gggttaggag gtgaattcct
     2341 ggctgggctc tcgaatgagc acgcgggcgg tggctcgcgt tgtgacgcat gcattgccat
     2401 tcgtaccgca acggggcgag ccatcgtggc gcgcgtggtg acctacggtg tagaggccga
     2461 gcctggcgat atcgatgtca gcccttcggt gtacgaggcc cttaacacgg gcgagtaccc
     2521 gcgcagcatg acgtggcagt tcactgattg ccccggcagc gcgaagctcc agtacgagtt
     2581 tcagacgggc gccaacgtgt ggtggacgag cttctgggta cgcaacggca aagtgccaat
     2641 cgagaaagtc gaagtgcaga gtgccaataa tcaagaattc ctgaccctac gccgcgaact
     2701 cgacggcacg ctcaatgcgg actcaggctt tggcgagggc acatttacct tgcggctgac
     2761 gggcgccggc ggtcgtgtgc tgacggactc gttcccgaag ttcgagccgg ggcagctgat
     2821 ctcgtccgaa aagcagtttt gaatcgtgag cgcgcctcga tgggcgtggc gctgtcggct
     2881 ttatacaagc gagctctcgc gtgcatgagc taagagccgc ccatgcacgt tcgaggaaca
     2941 ttcgagatca agatgcaggc agagcctcct tacgacgacg tcggtggggt cccgctgtcc
     3001 cgtgcctcat tcgagaaaca attctcggga gccctccagg cgacgagtca ggtgcagatg
     3061 ctggccgccc gtacgccgat cccgaactcg gcggggtatg tcgcgctgga acgaatcgtt
     3121 ggcagcttgg aaggcaaaca ggggtccttc gtggtcgtgc acgtgggcat catgacccgc
     3181 ggcgaaaggt cgctgcagat tcgaattgtg cctgactccg ggaccgatca gctgcaaggc
     3241 atcgcgggcc gcatggatat tcagatcatc gagggacagc acttttacga actcgagtat
     3301 tcgctgggag cgcccgcgca aaaccagggg aacgcgcagt aacgaccccg ttcgagagcc
     3361 attcgcgacg cagcgagtcg cggctgcaaa cgcggctttt ccaggcaagc ggcgtagaca
     3421 gacagccgga gcgggctgag caaaatgggg tgctgacctg acgaaggtcg tgctgttcgt
     3481 ttgaaataat cggtcatcac ggctatttga ccgaacgcaa cagagttgct ggtggaatcc
     3541 cccttggacg agctaaagcc gatgaggcct tccgattcga tgctcgcacc cgtgggtttc
     3601 caccacccac gcgcagcgcg tctctcgacc tcagctcccg acctcagctc ccgatttcag
     3661 ctctttaggt gtctggtcat gaatatccga ttccgtcctc ttacgttgct tggattcctg
     3721 tttacggctc aagttctcgt gttcgcgtgc ggaggcacgc ccggctctgg ttcgctgacg
     3781 accaacagcg gtggaaccag tgccatcccg agcccgatgg gtggcaaggt ctcctccggc
     3841 ggcacgcagg gcgaacccac gattccaagt gatgagggcg gagcatcgag cgagccaacg
     3901 gcaggggcga ccagcgcggg tggtgactcg agctccggcg gcacctcgaa ctcgggtggc
     3961 accaccggtg gcgtcgaaac cagcggcggc accactggca tgtcctcagc aggagctggg
     4021 aacgagggcg gggccgaagg cttacccgag ttctgtgatg acgtgacctg caagattgac
     4081 tgtacgaagc agggctcgtg tccggaacgt gtggagtgcc cgaaaggccg gaactgcgag
     4141 gtgacgtgcg gcgacgacgc atgtgccaag ggcgtccagt gcaacgacga gacgcaagcc
     4201 tgcaccgtga cctgccaggg acggaactcg tgcgccgggg aagtggagtg cgcgggcaag
     4261 aaggcttgcc aggtcaagtg caacggcgac gactcgtgca agtctcgtat ctacggcggc
     4321 agcggcgagc gcattcaagt ggattgcaac gcagcccgtt cctgcagcgg cggggtactg
     4381 gccaatgctc cgcaagttgc catcttctgc aacggcgaca gctcttgccg cggcgatttg
     4441 cagtgcaaca ccgatcgttg cgaagtgcgc tgcaattcgc agaactcctg cactgggagc
     4501 gtgcagtgca atgccggcca aaagatcggc gcctgcacgc gctgacggct cgctgacaga
     4561 gtgatggacc gtgctggcga gctggagctc accagcacgg tggtcagtaa ggctcggcag
     4621 gctgtccact gctcgtggta cgagcagcta gctcagcctt ggagccgtcg ccgcgcccgc
     4681 cgccaggcta tcgcgccgat cgccagcatc gcgagcgcca gacctcgttc gctcgtgcgc
     4741 cgcccgatcg cgcaatcgca cgaagagtcg tctttggagc tgtcgcctgc gctgctggtg
     4801 ccgccggtgg ccgtgtgacc tccggtgtgc gtagcgcccg ccgtattctc cgctcccgcg
     4861 ctcgagctgc cgccggaact tgcgatgcag cggaggcaat cataagtcgt ggtggtcgga
     4921 ccgcatgtcg cggttgttcc gcccgcggct gctcccgcgg cgcctccctc gcaattggtg
     4981 aatggcggtc caacgcgcgt gcacgtccgc gtcagacaca cgccttcgcg gctagtcgag
     5041 ccgccagtcg cgttatcgca agtctcgcct tcgttttgac aagcatcggg tggcaagaga
     5101 tccgcgtggg cgaccccgat cgctgccacc gggccgagca atccaaaaca aagcacgagc
     5161 gctgtaccgt atcgagtcat gcacacctcc tccactaaat aggattggga gtccgtctcc
     5221 ccagagaaac ccagaaatcc tcgaggccct tacgccggac tccaaacgat cgggccaaga
     5281 gaaaagcccg tagctcagct tgggtcacgt cgcaaagaag ggcgtggcaa aaagacaatt
     5341 ggacgagccg ggggacgtta cgtttcggta ccgaggttcg cgtgccaggg catcaggctc
     5401 gatcgcagca gccgcttgtg aaatcgcgag ccccgtccaa ggtttgatcc aatggtcggc
     5461 aattcgagcc ctttgtccac ggcctctcca gaggacttcc tgcatcgaca cgtggcggcg
     5521 ctccatcgca agctgcatcg cataggggta tcggcgagtt tcggtttgag cgaggcgggt
     5581 atcgaagcgg ccttcgagcg cgtgacgcca gcgggccatg gtcgcgagct gctcagcgaa
     5641 ctcaagtcgc tcggcagcta accctcggcg gcacacgcgc ggccttggcc acgcatgccg
     5701 caagagcgga gttcagccgt ggactttctg tc
//
LOCUS       DASTKU010000362         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1854374, whole genome shotgun sequence.
ACCESSION   DASTKU010000362 DASTKU010000000
VERSION     DASTKU010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854374"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFQ61_12475"
     CDS             <1..316
                     /locus_tag="VFQ61_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955318.1"
                     /translation="LTTVLSSRQAFHRSVLIEKLGVSDTATLERVHALSAGFIAKGFD
                     PESARRQALTILDGSVNLQASVMSFADSFWLVALVFLVTMPLLFLLGKGRPAGGAAVD
                     AH"
     gene            complement(367..1161)
                     /locus_tag="VFQ61_12480"
     CDS             complement(367..1161)
                     /locus_tag="VFQ61_12480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9955319.1"
                     /translation="MDLGLSGKRALVTGSTAGIGLAIATALAREGAHVIVNGRTQARV
                     DEAVNTIGRETSASRLSGIAADLGSEAGCKQLLGLVPEVEILVNNLGIFEVRPFEEIS
                     DRDWFRFFETNVLSGVRLSRAYLPAMRKRNWGRVVFISSESAIQIPTEMIHYGMTKTA
                     QLSIARGLAELLAGTGVTVNSVLPGPTSSEGVTGFVEEMAKARGVDAATIEAEFFKTA
                     RPSSVIQRFATPEEVAALVAYVCSVQASATTGAALRCDGGVVRAIV"
     gene            1412..>2042
                     /locus_tag="VFQ61_12485"
     CDS             1412..>2042
                     /locus_tag="VFQ61_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9955320.1"
                     /translation="MGIEDASLDQDAGASSSGESGETPSGGRSSGGTTSSYPQSGKSG
                     DSGNPNSGGMQPGAGGTRPGSGGRQSGGYSGTGGNSNTGQTQSGGATTTAGGTLNEAG
                     EAGAAESGGATTAGNAGRGGTTGSSGPTLHGKVIDFLGHGVPSAVVAVGNSETTTDSN
                     GLFTIQNVAATYDVAVTFSVPVRARTEEYTWVYQGLTRRDPTIQVYRGVE"
BASE COUNT          379 a          624 c          655 g          384 t
ORIGIN      
        1 gctgacgacc gtgctgtcgt cccggcaggc ttttcatcgt tccgttctga tcgagaagct
       61 cggtgtcagc gacaccgcaa ctctcgagcg ggtgcacgcg ctcagcgcgg gcttcatcgc
      121 gaaagggttc gacccggagt ctgcacgacg gcaagctctc accatcctcg acggcagcgt
      181 caatctgcaa gcgtccgtga tgtccttcgc tgactcgttc tggctggttg cgctcgtgtt
      241 cttggtgacg atgcctttgt tgttcctgct cgggaagggg cgccctgccg gcggtgcggc
      301 ggtagacgcc cactgagggc ctgaatttct cgttcaggcc gagagcaggt tcaactcagg
      361 gggcgactaa acaatcgctc gcacgacgcc cccatcgcag cgcaacgctg ctcccgtcgt
      421 ggccgaggct tgcacgctgc acacgtaggc gaccagagcc gccacctctt ccggggtggc
      481 gaagcgctgg attacggaag agggccgcgc cgtcttgaag aactcggcct cgatcgtggc
      541 ggcgtcgacg ccgcgggcct tggccatttc ttcgacgaac ccagtcactc cttcagatga
      601 agtgggcccc ggaagtacac tgttcacggt cactccggtt ccggcgagga gctcggccaa
      661 cccccgcgcg atcgaaagct gcgccgtctt ggtcatgccg tagtggatca tctcggtagg
      721 aatttgaatc gcagactcgc tcgagatgaa cacgacacgt ccccagttgc gcttccgcat
      781 cgcgggcaag taagcgcgcg ataggcggac accgctcaag acattggttt cgaagaacct
      841 gaaccaatca cggtccgaga tctcctcgaa cggcctgacc tcgaagatgc caaggttgtt
      901 gaccaaaatc tcgacttcgg gaaccaggcc aagcaattgc ttgcagcccg cttcggaacc
      961 gaggtccgcc gcgattccgg atagccgaga agcgctcgtc tcacgtccaa tcgtgttcac
     1021 cgcttcgtcc acccgagctt gggttcggcc gttgacgatg acgtgggctc cctcacgcgc
     1081 caaagcggtc gcgatcgcca agccgatccc cgccgtagaa cctgtaacta acgcacgttt
     1141 gccggaaaga ccgagatcca tgcgcgcatg ctcccatgcg cgggtcgcgc tgcaaagtgg
     1201 taacggtttc tactacggtt gagcgcggcg gccagttcgc gtttcgtctc gaagtggccg
     1261 agcgcggctt tgctcgcgga atcgttgcgc ggaatggcgc agcctcgagt atcatcgccg
     1321 agtcgtgcct agaaataagc gaagccgcag tcacctcggc cttggtctca ctggggtgct
     1381 tcttttcagc cctgtctcct gcgcatccat cttgggaatc gaggatgcca gtctcgacca
     1441 agacgcgggt gccagctcga gcggggagag cggggaaacg ccgagtggtg gtcgttcgag
     1501 cggtgggacc acgtcttcgt acccccagtc gggcaagtcc ggagactccg gtaacccgaa
     1561 ttcaggcgga atgcagccgg gagcgggcgg gactcgaccg ggatccggtg gtcgccagtc
     1621 gggtgggtat agcgggacgg gcggtaattc caacaccggc cagacgcagt ccgggggagc
     1681 gacgacgacc gcgggcggca ccttgaacga agccggagaa gcgggcgcgg ccgaaagcgg
     1741 gggagcaacg accgcgggta acgccggccg tggtggcacg acgggatcct ccgggcctac
     1801 tctgcatggc aaggtcatcg atttcttggg tcacggcgtg ccatctgcgg tagtggcggt
     1861 ggggaactcg gaaacgacca cggactccaa tggcctattt acgatccaga acgttgccgc
     1921 cacctatgat gttgcagtga cgttttcggt tccggtgagg gctagaaccg aagagtatac
     1981 ctgggtctat caagggctca cccggcgtga tcctacgatc caagtctatc gcggcgtgga
     2041 ga
//
LOCUS       DASTKU010000363         7388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_961056, whole genome shotgun sequence.
ACCESSION   DASTKU010000363 DASTKU010000000
VERSION     DASTKU010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7388
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961056"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VFQ61_12490"
     CDS             <1..749
                     /locus_tag="VFQ61_12490"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9955321.1"
                     /translation="NGRPKLIVPYRNGLTANALTAARASCTQIPAHHAAGDPVLIGLW
                     EQPTVISSTPEPTCTIGVEFHPEGLSNLFSDCLFQGASELTQRIVSLSDVLGATGRAL
                     GEHVVNACSPVAAAEIVQRFLRTRLARCPTEPPLIEAALGLLRNSGYRMEVSELERRM
                     GYSRRYLQSLFQRRVGLPPKRLASVLAFERLYRRFSQQKSVELLRRDALELFYDQSHF
                     IRNFRRFTGSSPSAFAELDNEFGRLFYVTR"
     gene            complement(908..2284)
                     /locus_tag="VFQ61_12495"
     CDS             complement(908..2284)
                     /locus_tag="VFQ61_12495"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HET9955322.1"
                     /translation="MSPIPELIELPATDGPFEAPNASDVDPAPGVVEIELEAKPTNVE
                     LAAGHVVAIWAYNGSLPGPTIRARVGDTLRVRFKNSLPEPTTIHWHGLRVPADMDGAP
                     ARQVPAGAEFIYEFILLDAGTFWYHPHVHSEQQVERGLYGAIVVTGADEPATSSDHVI
                     VLDDVLLGADWQLVRFTPMQALSGRQGNLVLVNGRAYPIAHLGAAGAHRFRFINAANA
                     RYFRIALPGESFVQIGSDGGLLEAPRAREELLLVPGERADVVWEAPAGGSPSAERPTF
                     EWRTLRYDRGHHTGDLPETSLFRATLEPGSFDPGSTAPLAGTPPHWATIPELPAPSRE
                     RQLRLEQATLAGRGMGPVFAINGQTYPNVTPLVSELGAVEQWAILNDTDMDHPFHLHG
                     FRFQLVEAPDPELLTFRAWRDTVNVPARQSLKFRVQFEAHPGTWMFHCHILEHAENGM
                     MGELEVMP"
     gene            complement(2383..2934)
                     /locus_tag="VFQ61_12500"
     CDS             complement(2383..2934)
                     /locus_tag="VFQ61_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955323.1"
                     /translation="MESIELDQVMRRARRAYELGRARRALMGVAPVVFIVACAACVTH
                     RPVSTLWFGLAAVAVGGLMLWYGRDPQRAVLPGVLAGLVPLILAIGANRIHVCGPHGC
                     GTLCVPACAIGGVVAGLAVAGVGQQRRAGAWFWLSASGLALLTGAMGCACIGYSGVLG
                     LLGGFLAGLVPALVRRALGRAPR"
     gene            complement(2921..3451)
                     /locus_tag="VFQ61_12505"
     CDS             complement(2921..3451)
                     /locus_tag="VFQ61_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9955324.1"
                     /translation="MPQNSEELDALMKRLAEGDRSVFTRVFELLWAPTKRLCSNLLKN
                     DADAADAAQEAMQKILERASDYDDTRPALPWALAIAGWECRTLARKRVRRREANAADL
                     PESAGENPEPEFVQRDLMAAALAALGELSESDRETLVATFWDEAASVSGATLRKRRER
                     AIERLRKTFKRLYGID"
     gene            4268..4510
                     /locus_tag="VFQ61_12510"
     CDS             4268..4510
                     /locus_tag="VFQ61_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955325.1"
                     /translation="MLRTQGSRLEEHLTSTSGRTELATEPPLDFLTYLEQRYQANRAL
                     VTSLLGEWLVRYEPCEQRAILVAPSALGNRVAPGAE"
     gene            4649..5041
                     /locus_tag="VFQ61_12515"
     CDS             4649..5041
                     /locus_tag="VFQ61_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955326.1"
                     /translation="MTKTTDTTAPADASSPAPVTDQVMQQLLGASPPRIVFLLRRGPR
                     YDDPATLPLQWEHARNMFSLLRAGTLLSVTALMDGTDVLGVGVLASGTRESAEALLAQ
                     DPAVRGGRVTVQFHTGASFRPEDLATAG"
     gene            complement(5094..5549)
                     /gene="aroQ"
                     /locus_tag="VFQ61_12520"
     CDS             complement(5094..5549)
                     /gene="aroQ"
                     /locus_tag="VFQ61_12520"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET9955327.1"
                     /translation="MTALKILVLSGPNLDRLGRREPHIYGSTTLAQIHSELESFAEQR
                     GVRVECRQSNHEGDLVDWIGASPDDGFHGILINPGALTHTSYALLDAIKGCGLPTVEL
                     HLSNPDAREEFRKRSRVAPACLGRIAGFGARSYWLALEGLLDHLARVRS"
     gene            complement(5559..5921)
                     /locus_tag="VFQ61_12525"
     CDS             complement(5559..5921)
                     /locus_tag="VFQ61_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955328.1"
                     /translation="MFKEALRSVVEGTDGGLAGLVMDLEGIALETYARQDSGFDIESV
                     GAEVSVVVKAIQRASEMLEAGPTREVAFKSDRLITLIRIINNSYFVAMALAPDGNLGK
                     GRYLLRVLAPRLEQELAS"
     gene            6185..>7388
                     /locus_tag="VFQ61_12530"
     CDS             6185..>7388
                     /locus_tag="VFQ61_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HET9955329.1"
                     /translation="MRARAGDPPRTMTQKILAGRADDPNLAGELVRVKVDQVVLARDP
                     AAAMGRVLELGGKKCAVEVGIAYDTACVTTPGDLPSSAAVKSLKEALNLGMLVGRPGI
                     GFPAAVHLERFGSPARLTVTDEPRLAAVGGAGMLSLVVPPSQLAEAIVAGAIWLRPPR
                     SVQVLLSGRVRPFVCVRDVGLELLRRGLRETIRRVDETYGAPVVVEFTGPSARLLSVA
                     ERAVLSALAPSLGASAAVFVSDEKTEVYLRDQRRSKAHRGLLPDPGAPCDDVISVDLS
                     AVDPLVMDADGNVRQVRELAGETVTQVVLGGDSGASLRDLLAAAALLKSKRVPPKLDL
                     LLSPPSRQALEVLAQSGALVDLIATGARLIEPDPRLVTGELYPPLGSGLSLRTFDPEP
                     NLAQTPKFV"
BASE COUNT         1354 a         2431 c         2316 g         1287 t
ORIGIN      
        1 cgaatggtcg ccccaagctg atcgtgccct atcgcaatgg gctgaccgca aacgcactca
       61 ccgccgcgcg tgcctcttgc acgcaaatac ccgcgcacca cgccgcgggt gatccggtcc
      121 tgatcggact atgggagcaa cccaccgtta tctcgtcgac gcccgaaccc acctgcacga
      181 ttggcgtcga atttcatccc gaaggattat cgaatctatt ttcggattgc ctgtttcagg
      241 gtgcgagcga gctcacacaa cggatcgtct cgctctcgga tgtgctcggc gcgacggggc
      301 gagcgctcgg cgagcacgtc gtcaacgcgt gctctccggt agccgccgcc gaaatcgtgc
      361 agcgcttttt gcgcacgcgg ctcgcgcgct gcccgaccga gccgccattg atcgaggccg
      421 cgctcggact cctacgaaac agcggctacc gcatggaagt cagcgagctc gagcgacgca
      481 tgggatactc ccggcggtac ctgcaaagct tgtttcaacg ccgcgtcggg ttacctccga
      541 aacggctcgc tagcgtgctc gcgttcgagc ggctctatcg ccgcttctcc caacagaagt
      601 cggtcgagct gctgcgtcgc gatgcgctcg agctgttcta cgaccaatcg cacttcatcc
      661 ggaacttccg gcgcttcacc ggctctagcc cgagcgcctt cgccgaactc gacaatgagt
      721 tcggccggct cttctatgtg actcgctgag cttcagctca cacgcccagt tttgggatgg
      781 ccaccgaaca acgtgcgcga cgcgcggcgc ggtgcccgac cgcgcagccc aagtgcccgc
      841 agtcccgtcc catcaccgcg ccggccccca atgaactcac cgtcggagcg cgcgggcctg
      901 cctactctca cggcatgacc tctagctcac ccatcatgcc gttttcagcg tgctccaaga
      961 tgtggcagtg gaacatccag gttccggggt gggcttcgaa ttgcacccgg aacttcagcg
     1021 actgtcgcgc tggaacattg accgtgtcgc gccaagctcg gaaagtgagc agctcgggat
     1081 cgggcgcttc taccagctga aatcgaaagc catgcaagtg gaacggatgg tccatgtccg
     1141 tgtcattgag gatcgcccat tgttccacgg cccccaactc gctgaccagt ggagtgacgt
     1201 tcggataagt ctggccgttg atggcgaaca ctggccccat ccccctgccc gcgagcgtag
     1261 cttgttcgag ccgcagctgg cgctcgcgac taggtgccgg cagctcggga atcgttgccc
     1321 aatgtggcgg cgtcccggcg agaggggccg tcgatccggg gtcgaacgag cccggttcga
     1381 gtgtcgctcg gaacaggctg gtctcgggca aatccccggt gtgatgacct cgatcgtagc
     1441 gcagcgtccg ccactcgaac gttggccgtt cagccgatgg cgagccgccc gcgggtgcct
     1501 cccaaactac gtccgcacgt tcgcccggaa ccagcagcaa ttcttcgcgc gcgcgcggtg
     1561 cctcgagtaa gcccccgtca ctgccgatct gaacgaacga ctcccccggc aaggcgatgc
     1621 ggaagtagcg ggcgttggcg gcgttgatga agcgaaatcg gtgagccccg gccgcgccga
     1681 ggtgcgcgat cgggtacgcg cgaccattga cgagtacgag gttaccttgg cggcccgaga
     1741 gcgcctgcat gggggtgaaa cgaaccaact gccagtccgc cccgagcaac acgtcatcca
     1801 acacgatcac gtggtcgctg ctcgtcgcgg gctcgtcagc gcccgtcacg acgattgctc
     1861 cgtacagacc tcgttcgacc tgctgctcgg aatgaacgtg cgggtgatac caaaacgtgc
     1921 ccgcgtcgag cagtatgaac tcgtaaatga actcggcccc ggctgggacc tggcgcgctg
     1981 gtgctccatc catatctgcc ggcacccgca agccgtgcca atgaatggtc gtgggctccg
     2041 gcagcgagtt cttgaagcga acgcgcagag tgtccccaac ccgagctcga atcgtggggc
     2101 cgggcaacga accgttgtag gcccaaatcg cgacgacatg gcccgccgcc agttcgacgt
     2161 tggtcggctt cgcttcgagc tcgatttcga ccacccccgg cgcgggatcc acgtcactcg
     2221 cattcggcgc ctcgaacggt ccatccgtcg caggtagctc gatgagttcg ggaatcgggc
     2281 tcatgtctcc catgctcatg tcgcgtggac cgctgcacgc gttgataagc cccaaactcg
     2341 agctcgcgag aaccgagcgc caccatggct tactctcgaa catcaacgtg gcgctcgccc
     2401 gagagcccgc cgcaccagcg ccggcacgag cccagcgaga aagccaccga gcagccccag
     2461 cactccagag tatccgatgc aggcgcagcc catggccccc gtcaacaacg ccagccccga
     2521 tgcagaaagc caaaaccagg ccccggcccg gcgctgttgg ccaaccccgg ccacggccag
     2581 gccagctacc acgcctccga ttgcacaggc gggcacgcac aacgttccgc agccgtgggg
     2641 cccacagacg tgaatccgat tggcaccaat tgccaaaatc aacgggacca aacccgccaa
     2701 tactccgggc aacacggcgc gctgcggatc gcggccatac cacagcatga ggccgcccac
     2761 ggctaccgcc gcgagcccga accacaacgt agatacgggt cgatgcgtga cacaggcggc
     2821 acaagccacg atgaaaacga ccggcgcgac ccccatcaga gcccgccgcg cacgcccgag
     2881 ctcgtaggcg cgacgagctc gtcgcatcac ctgatcgagc tcaatcgatt ccatacaacc
     2941 tcttgaatgt cttgcgcagc cgctcgatcg cgcgttcgcg acgtttgcgc agcgttgccc
     3001 ccgagaccga cgcggcctcg tcccagaaag tcgcgacgag ggtctcacga tcggactccg
     3061 agagttctcc gagggcggcc agggcggcgg ccatcaagtc tctctgtacg aactcgggct
     3121 cggggttctc gccagcagac tctgggaggt ccgccgcgtt cgcttcacgc cgccgcaccc
     3181 gcttgcgcgc caacgtgcga cattcccagc ccgcgatggc gagggcccat ggcaaagcag
     3241 ggcgggtatc gtcgtagtcg gaagcgcgct cgaggatttt ttgcatggct tcctgggccg
     3301 cgtcggctgc gtcggcgtcg ttcttcagca agttcgagca aagtctcttg gtcggcgccc
     3361 agagcaactc gaagacacgc gtgaagacgc tgcggtcgcc ttcggcgaga cgcttcatca
     3421 acgcatcgag ctcttcagag ttttggggca tgggagcatc gccgaccgca gcagcctccg
     3481 cgggaggtta ccaggtcaag acggtcccag agggcgtggg agcgggaaca cggtgggagc
     3541 ggcgggggct aaggcttggc gggctggggc gagcgagtgc tgcacggatc gaccagtggc
     3601 gcggaattgc gcggctggcg cggcctttca tcgtcgtagg cgggaggggc gccaggcgtg
     3661 acccggaaaa ttcttcagtt cgccggccac atgcccaccg gtctaccgtg tccgccggtc
     3721 tgcgtgtccg ccggtctgcg tgtccgccag gtggggtgtc cgccagttgg gctcccaacc
     3781 atctgtccgg agaacgacca ctctcgagca ccgcctcgac gactgggcaa gaagagtccg
     3841 aagcgtcgag tgtcatgcgc gaacttgcgt gcgacgcatt tgtatgctgc gcgacgaaag
     3901 cgtcgccccg gctcgctcac ggctcgtact gctgatttgc gaataggttt cgcggagcgc
     3961 tgcaccttta cgaggagtgc ggtcgtcgcg tccattcacg acctccgcgc cagccggcgg
     4021 agctcggaga ccgacgagaa acggacacag aacttcgtca atagagctcg ttttcagcgg
     4081 ctcgagaata ctttcggacc cagcgcctgt acgcacgaac tgcagtgcgc actcgcaatc
     4141 tcggcgcgtg aactcgaggt ttcgaagttt catatacaca ctcccgacat caaccctcca
     4201 cgaattcgga tttggatcgg agcttgcaac tcagagactc aggcgcagca accttcaagg
     4261 ggccgacgtg ctccgaaccc aaggcagtcg attggaagag catctcactt caaccagcgg
     4321 ccgcaccgag ttggcaactg aacccccgct cgactttttg acgtacctcg aacaacgcta
     4381 ccaagcgaac cgtgccttgg tcacgagcct gctgggggaa tggctggttc gttacgagcc
     4441 gtgcgaacaa cgtgcgattt tggtcgcacc ctcggcgctc ggaaaccgag tggctcccgg
     4501 cgccgaatga ggcggccggt gaagccccgc ggctaaaggg aggatcaaga cagcgcgcac
     4561 gctttcatgc tctcccggag cgctcccgtt ccgattcgta caatacgagt tggggaaaac
     4621 caggcaggtt gtgtcgagga agaacacgat gacaaaaacg accgacacga cagcccctgc
     4681 cgatgcaagc tcgcccgcac cggtgaccga ccaagtgatg caacagctgc tcggagctag
     4741 cccgccgcgc atcgtcttct tgttacggcg aggcccacgc tacgacgatc cagcaacgct
     4801 cccgttacaa tgggaacacg cgcgcaacat gttctctctg ttgcgcgcgg gaacgctgct
     4861 gtccgtcacg gcattgatgg acggtacgga cgtgctcggg gtcggagttt tggcgtcggg
     4921 cacgcgggag agcgccgagg cattgctggc tcaagacccg gccgtacgcg gcggccgcgt
     4981 gaccgtgcag tttcacaccg gcgcctcttt tcgccccgaa gatctagcca ccgcgggctg
     5041 agcgccagcg cactgtgggg ctgaggaaca gtcactccac gagcgaacgc acctcagctc
     5101 cgcacgcgcg ccaggtgatc cagcaagccc tcgagcgcga gccagtagga ccgggcgcca
     5161 aagccggcaa tccgcccaag gcaagccggt gccacccgag atcgcttacg gaattcctcc
     5221 cgcgcatcgg ggttggaaag atgcagttcg acggtcggca agccacaacc tttgatcgcg
     5281 tcgagcagcg catacgacgt atgcgtcaac gcgcccggat tgatcaaaat gccgtgaaaa
     5341 ccgtcgtccg gtgaagcgcc gatccaatcg acgaggtcac cctcgtggtt tgactgccgg
     5401 cactccacgc gtaccccacg ctgctcggcg aaagactcca gctccgagtg gatctgagcc
     5461 agcgtggtgc tgccgtagat gtgtggttcg cgtcgaccca gacggtccaa gttcggcccg
     5521 ctgagcacca aaatcttcaa ggccgtcacg acgcagactc agctcgcgag ctcttgctcg
     5581 agccgcggcg cgagcactcg aagcaggtag cgccccttgc ctaggtttcc atcgggcgcg
     5641 agcgccatgg ccacgaagta gctgttgttg atgatccgaa tcagggtgat caagcgatcc
     5701 gacttgaacg ccacctcacg ggtcgggcct gcttccaaca tctcgctggc gcgctggatg
     5761 gccttgacca ccacgctcac ttcggcaccc accgattcga tgtcgaagcc cgagtcttgg
     5821 cgcgcatagg tctcgagcgc gatgccctcc aggtccatga cgaggccagc taggcctcca
     5881 tcggtgccct cgacgacgct ccgaagagct tctttgaaca tcgggctagc ctagccggtt
     5941 tcgttttggt cgtgaattgg gtgtgtggtt ccgagcactt catgaagtca gacccacatt
     6001 gcccacgtga aggtcgacca gccaatcaga gaaggagagc ccgggggctt gtcgccgcgg
     6061 caggactctg taatggccga acggcgcggg gccgtacgac cttgcttgac ccttccgcgc
     6121 gcaaacggct accttcgccc tcgcccgatt cacagccaag tcgggcacta ggaacggaaa
     6181 tccgatgcgt gcgcgagcgg gggatccgcc ccgcacaatg actcaaaaga ttctcgccgg
     6241 gcgcgcagat gacccgaatc tcgccggcga actggtgcgt gtgaaggtcg accaagtggt
     6301 cttggcgcgc gaccctgctg cggcgatggg gcgcgtactg gagcttggtg gcaagaagtg
     6361 cgcggtcgag gtgggcatcg cctacgatac ggcctgcgtc accacaccgg gcgatctgcc
     6421 ttcatccgcg gcggtcaagt cgctgaagga ggcgctcaat ctgggaatgc tcgtgggcag
     6481 accagggatt gggttcccgg cggcggtcca tctggaacgc tttggtagcc ccgcgcggct
     6541 caccgtcacg gacgagccgc gtctggctgc ggttggcggc gcgggcatgc tctctttggt
     6601 cgttccccct tcgcagctcg ccgaggccat cgtggcgggc gcgatttggc tgcgtccacc
     6661 gcgtagcgtg caagtgctgc tatccgggcg cgtgcgtccc ttcgtgtgcg tgcgggacgt
     6721 ggggctcgag ctgctgcgac gcggtctacg cgagacgatt cggcgggtgg atgagacgta
     6781 tggggccccc gtggtcgtgg agttcacggg cccgagcgct cggcttctat cggtcgcgga
     6841 gcgcgccgtg ctctcggccc tggctcctag cctaggcgcg agcgcggccg tgttcgtcag
     6901 cgacgagaag actgaagtct atctgcgaga tcagcgccga tcgaaagccc accgtgggct
     6961 tttgcccgat ccgggcgcgc cttgcgacga cgtcatcagc gtagatctct cggccgtcga
     7021 cccgctggtg atggacgcgg atggcaacgt acggcaggtg cgcgagctgg ccggcgaaac
     7081 ggtgactcag gtcgtgttgg gcggagactc gggcgcgtcg ctgcgggatt tattggccgc
     7141 cgcggctctg ctgaagagca aacgagtacc tccaaagctc gacttgctgc tctcaccgcc
     7201 ttcacgacaa gcactcgagg tgctcgcgca atcgggcgcg ctggtggatc tgatcgcaac
     7261 gggtgcccgc ttgatcgagc ccgatccgcg attggttacg ggcgagcttt atccaccgct
     7321 gggctcgggg ctctcgctgc gcacgttcga cccggagccc aatttggcgc agacgccgaa
     7381 gttcgtgg
//
LOCUS       DASTKU010000364        12342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1854415, whole genome shotgun sequence.
ACCESSION   DASTKU010000364 DASTKU010000000
VERSION     DASTKU010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12342
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854415"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VFQ61_12535"
     CDS             <1..220
                     /locus_tag="VFQ61_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955330.1"
                     /translation="ANNEIKIWINSEAKTDLTVNTKSHGGNSTDFVFPTFNNIWFGWW
                     LYQDGPTPNMFDLWIDDIALSSDRLGCQ"
     gene            1264..1845
                     /locus_tag="VFQ61_12540"
     CDS             1264..1845
                     /locus_tag="VFQ61_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955331.1"
                     /translation="MSLEDYLGCLLGADGAVRCWGTALDQYDPVEPTLVPSLSGVIQV
                     SVGLGYACARTSGDGVKCWGHNQHGNLGDGTTEERLRPVAVSGLTNALEISAGAFHAC
                     AVLSDGTVRCWGNNRSGQLGDGTQGLNAISSTPVPVSGLRDVIHVAAGEYYTCVERKN
                     GDVMCWGYNGFGALGTGTTQDEIHPVLVDLGGG"
     gene            2534..3703
                     /locus_tag="VFQ61_12545"
     CDS             2534..3703
                     /locus_tag="VFQ61_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955332.1"
                     /translation="MMFRSATVSVVLAGLSGLFVGCGSESGSPSATGGSPGAGGGLGS
                     SGSLNASGTGNLATGGALASGGASGGGPSSGGAVSSGGLQSSGGVSSGGAANGGVFGS
                     GGSGTNSGGLSAGGAASGGTSAAGGSGGGFGGRGGKGGASSGGMSAGGAMSSGGASSG
                     GASTGTGGGTGSGSAKFSFFVTSLEAMRRLSKNQNGFGGDFRYGEADGLAGADKICTE
                     IAESSMPGSGAKGWRAFLSTSKVNAIDRVGMGPWYDREGRLVATGVQGLLRDRPDGDA
                     AVVSDLPNESGVPNRAGTGGGMDDNHDTVTGTNTQGKWDGGSTCSDWTSTTTSGGPRV
                     GHSWPANSGRNWMQAHNAPGCAPGVNLSLSSGAGSGNGIGNGGGYGGIYCFALMP"
     gene            complement(3800..4195)
                     /locus_tag="VFQ61_12550"
     CDS             complement(3800..4195)
                     /locus_tag="VFQ61_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET9955333.1"
                     /translation="MGQTESTTSTPNASEPRTYHGSCHCGAVKYDVDMTLDQLISCNC
                     SICSRTGAILGFVTSDKFKLLSGEDALSDYQFKTKTIHHLFCKNCGVRSFGRGKNTDG
                     TPMYAINVRCLDGVDVNQLKVQQFDGKSM"
     gene            complement(4271..6157)
                     /locus_tag="VFQ61_12555"
     CDS             complement(4271..6157)
                     /locus_tag="VFQ61_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955334.1"
                     /translation="MELVNKTARRIRIAHRSNGPPKILAPFARMSTTPSAVRELDVDP
                     WIALRFLSVERPLERDRPRLDLFLLAALPVALASGLVVYATAPNGFSLHQLAWLTLVL
                     TTMLTAFLWAVPLLRQYIPGLQSIGDFGDARDFIFRTSGILTVMLTGFGLPALAAWLQ
                     CNPQGSISALIQHMLSASSSNAALRGTALLVLYATFVGSAATLPALLFFLFSELRRDV
                     AKEQYRRDLLRLDPSLRNLEEVDTKYAAHLDEALSARKGPLFFVPVITSTFLLSLGWV
                     LATLPSMHVMAQVFGTNIATPVEVHKFLIPNREPFTFAFFGAYFFSLNMVFRRYVRAD
                     LGPKAYSHIAVRIIVASIFAWVISQTYPELGAPPVPLATDKAGEALAQSPWHLLLLVF
                     FVGIVPDTGVAFLHDLLKNRWVGAIVPSLKNRDPLNVLEGITLYDTARLLEEGVENVE
                     NLAHHNLTDLLLRTRIPASRLVDLVDQAILYLHTREDASESESGGALLGRLKANGVRT
                     ASDLLAAAARDAEGLESIATPTRIRLIVAAIEDDEWMPQIRSWRGLNATYGLVCDLND
                     VCTDVDRHGSTRLPHAQVTPTQRRPSPEPTTLPATEPATQPTDPPVSTSGRFSARGAT
                     GLQR"
     gene            complement(6406..8337)
                     /locus_tag="VFQ61_12560"
     CDS             complement(6406..8337)
                     /locus_tag="VFQ61_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET9955335.1"
                     /translation="MIGLVNLGKSYGARTLFEGTSLELLAGARYGLVGANGSGKTTLL
                     RILSGDEPASDGQVTLPKQARLGVLRQDQFANDSEAILDVAMSGDTLVTSALAEQARL
                     IESGAQPERVAELEDRIASLDGYTLKARASHVLEGLGIPVPQHSKPLWSLSGGFKLRV
                     LLAQVLIGGPDILLLDEPTNHLDILSIRWLEKFLAGYRGCAVVISHDQRFLDNVATHI
                     LDVDYQTVTLYPGNYSAFEKSKAETHARKAAEIARAEAAIAEKKAWIERFGAKATKAR
                     QAQSRLKQLERIEVDELEQSTRRAPHFHFEQERPSGKDVLEMQGISKSYGEKAVLRNV
                     SLTLRRGERLAVIGPNGLGKSTLLKIVTGKLEADAGKTQLGYEARLGYFAQDHHELLS
                     DPDARVLDVIWDTIPQETTSYVRGRLGNLLFTGQDVEKKVGSLSGGEAARLVFCRIMV
                     QKPNLLVLDEPTNHLDIESIQALIRALQTFEGTVLFVSHDRAFVAALATRILEVTPGG
                     FRAFNGTYAEYLAQSGDDHLDSNAVALRDKQEKSKAQAEKATSSALSWEEQKKRKNRL
                     KQLPNERDRVLAAIESAEARKAAIQAQWGDPTFYEQTAPSKIAELENEEKQLEAQIAS
                     WMAEWEALELELEALSNSG"
     gene            complement(8395..10446)
                     /locus_tag="VFQ61_12565"
     CDS             complement(8395..10446)
                     /locus_tag="VFQ61_12565"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9955336.1"
                     /translation="MRSIRLSPRFTSLHFAARFGWLVLASGTACDDGTRSPAGASGGT
                     TSGGSAAITGGSGGSGALPGTGGSTTGATECGAPDASLAKRERSELFESDHVPVFDLV
                     LPPDAWSELKRTARDEKYVEAKACYEGRAVGNVGLRFKGSYGTLLNCFDAAGNQKCPK
                     LSMKVKFDKYTADQRFFGMKVLNFHSMIWDPSHLRERIGYRLFRDMGVVAPQSFFATL
                     RVNGETQGLFSMVEEVDGRFTDDRFPKNGDGDLYKEAWPTSTDPEYYADHLETNEETA
                     THEVPLSLGAALNQASSAAEARSKLAEFVDLPNLYRYMAVDDAILNVDGVTAVYTDEN
                     SSYALIHNFYLYKEETRPQLWFIPWDLDSTFEFGTFGAVPHWTEKPADCSLHYPVWNG
                     GGIVYAPGCNILFQALASDLTEYRSAVQELLDGPLAEKPLLERIDQQAELIRAAAESD
                     PHGPSPETFEAAHQHLKTRIPLIRGRMQSLMEGRQPVPFAFEPRALNDFENVEEFELL
                     LGATLMFNQNSRVTYTLNRTDPLAGARDLRLEFSYRNETKPWDQWIYFSIPFRGAQID
                     ANALTGVRLRVRADRARTLRLDLESPRQTGTSAGVRFGWDIPVSDQSRLVEVHFADAK
                     VQSWAVDQGLDPHDDPHEVLGTMIGLALNPSCEGRNSAGFLPEGSSDDGQIELDDLEL
                     F"
     gene            complement(10629..10994)
                     /locus_tag="VFQ61_12570"
     CDS             complement(10629..10994)
                     /locus_tag="VFQ61_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955337.1"
                     /translation="MSTESPLSTEPEPNTAPLPTLHEAWLTRAEVEQLAKDVENAAEL
                     LDITLKSGGTRRAELAAGETCSLRQAVARLLRREVVGVQIRYRHAGEEWWDTLLSGES
                     GIRLVRVNHSRAVTPPGRG"
     gene            complement(11024..11797)
                     /locus_tag="VFQ61_12575"
     CDS             complement(11024..11797)
                     /locus_tag="VFQ61_12575"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF015828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HET9955338.1"
                     /translation="MTFDRPSVSPKRATWFGIIAVLTLSAVGFLGETARPSPAPWRPP
                     PAGSAVNAAVPRARSYSELGEVRRGPNGAMYGNAFVALRAAPDPIDSRLVPPAERIAT
                     VEERSRRRAYAGAPPVIPHAIAQRELNDCVSCHAYGVRIAGRTAPLPSHELYTNCVQC
                     HAVAPEFESAQVPDPKLSPEAGPSKVNEFAGLQSYGLGTRAWPGAPPTIPHPTQMSSQ
                     CTSCHGPQGPNGLRTPHPDRQSCLQCHARREPADSPPAL"
     gene            complement(11801..>12342)
                     /locus_tag="VFQ61_12580"
     CDS             complement(11801..>12342)
                     /locus_tag="VFQ61_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HET9955339.1"
                     /translation="VVEKDGVFRETKFRFAAFDDPYVKPGQDIDFYHSPTKDGRAQVW
                     FRPYVAPPERPDDEYPFWLCTGRVIEHWHSGSMTMRIPPLRRSMPNAYVEMHRDDAAQ
                     LGIQNGEHVIVQSRRGEIELPVWLDGRGSPPRGSLFVPFFDERLLINHVTLEAYDPFS
                     KQPDYKKCAVRVRRVARKT"
BASE COUNT         2224 a         3557 c         4180 g         2381 t
ORIGIN      
        1 ggccaataat gagatcaaga tttggatcaa cagcgaagcc aagacggatc tcacggtaaa
       61 tacgaagtct catggcggca actcgacaga cttcgtcttc ccgacgttca acaacatctg
      121 gtttggatgg tggctgtatc aggacggccc gactcccaat atgtttgatc tgtggattga
      181 tgacattgcg ctgagctccg accggcttgg gtgccagtag ccgctgccgc tctgcatagc
      241 ctttggaatg agggcgggcg gggttaggag cccgctctcg ttctagtatc gccgggacaa
      301 ctctgcggct ctgcgcttgc ctcttgccta gtgtcagggc taatttcgag cccgggtaca
      361 atgagcaggg cgcgctcgga taggaacatc acgatgtatg cggtgacggg taggggtggg
      421 aggtgggctc tgttgctaat cgctgccctc ggcaccgtca ctatccaatg caatgtgggg
      481 ggaaaccgag tcttccacga aattgaagac gggcagagcg gaagcgccgg tcgcgacgac
      541 agcgagggca actcagaagc ctccggcgga gcttccggag gcggggacgc gcgttcggag
      601 agcgccggcc gcgatgctgg ccactccggt cccgattcat ctagcggttc tacaggcgat
      661 tccgggcgtg tcgctgaggg ggctgcaggt tatgcgccgg atgacgaggg aggggcacgc
      721 ggcggttcag gtggcgatga cggcacgatg tctaccggtg gtagctccgg ggaaagacct
      781 gaattggaag ggagtgatga cggtggggcg aagggccagt ccacaccacc gcgaagtcgt
      841 atcgccgcgg gaagggacca cgcctgcgcg attcgagccg acagagcggt cgtttgctgg
      901 gggaacaaca ctgtcggtca actgggagac ggaagcagaa cgagttcctt caaaccagta
      961 caggttcccg gcattgccaa catcgaacaa atcgcggctg gcggtggatt gtcttgcgca
     1021 atatcgacga caggagactt gtactgttgg gggaacaacg agaatagctc tggactcgac
     1081 caagttggcg cagcccagct tctgccgcgt acggttttgc cggagtcaat ggtgcggagt
     1141 gtggcattgg gacgctcccg cgggtgtgct gtcacaggag caagagttct ttgttggtct
     1201 acaccaccga actggacagc cacggcaccc actatctttg cctcaacgat tgcggtggat
     1261 gccgtgtccc tcgaagatta cctcgggtgt ttgctgggcg cggatggtgc ggtgcgctgt
     1321 tggggaacag cgctcgatca gtacgatccc gtcgagccta cgctggtgcc cagcttgagt
     1381 ggtgtgattc aggtgagcgt cggattaggt tatgcatgtg cgagaacgag cggtgatggg
     1441 gtgaagtgtt gggggcacaa ccagcacgga aatctcggag acggcactac tgaagagagg
     1501 ctgcggcccg tggccgtatc cggcctcacc aacgcgctcg agatctctgc gggagcgttt
     1561 catgcgtgcg ctgtgctgtc ggacggcacc gtccggtgct ggggaaacaa tcgatctggg
     1621 cagttgggtg acgggacaca gggactgaat gcgatcagct caacaccggt tcccgtatcc
     1681 gggcttcgtg atgtcatcca cgtagcagcc ggtgaatact atacttgcgt cgagagaaaa
     1741 aatggggacg tgatgtgttg gggctacaat ggcttcggtg cgctcggaac gggcactacc
     1801 caagatgaaa tacatccggt gctggtggat ctgggtggtg gctagagcaa ggtctcgaat
     1861 ggagattggg tgattgtcta gaggtgttcc gctgaatctc cctgcgctaa gcccaacacc
     1921 tgccggtgat gacgatcgga gatcaatcgg gctccacggt tcgctatgac ggccgcagct
     1981 cggcatttca cggggcatcc gcacgcgcac gcgcacgcgg accgtgacgt gtaccggctc
     2041 ataacgtcgt gatcgtcgcg ccgcgtatgc ggcaccgaat cgccccaagt atgtgtgtgc
     2101 gaaaggacaa agcactcact ggactacgcg gcgcggtcac ctccgcgccg aatcctgggc
     2161 gcaaagctgg ccttggcggg ctttctgaag tctttgacag tagtgccagc ggtgacgtta
     2221 ttgagtgccg tttgcgcggt attgggggga tggttggcgg gatgggactg tgctggtggg
     2281 tgctgggacg agcggcgcgc ccgtggttgt gggcggcaag gaaagggact ttccttcgac
     2341 ggggataatg accgcatcac agtgccgggt gcgattcggg cggattcttg tgattgggtt
     2401 gcgtctttgc tggcgccgcg ggcgctggac ggaaagattt ttgcgttttg gtgaacgctg
     2461 caggggtggg ctgggcacat gacatttgga gcctctgcgg cgcgcttcgt ggacgcaggc
     2521 tctggagctc agcatgatgt ttcgttcggc gacggtttct gtggttctgg cgggtctctc
     2581 aggcctgttc gtgggatgtg gatcggagag cgggtctccc agcgcgacgg gtggtagtcc
     2641 tggcgcgggc ggaggtttgg gctcgagcgg ttcgctgaac gcgagtggga cgggcaatct
     2701 agctaccggc ggggcgttgg cttcgggtgg ggcttctggc ggtgggccga gctccggggg
     2761 cgcggtgagt tcgggcggct tgcagtcctc tggcggcgtg agctccggcg gggcggcgaa
     2821 tgggggagtg ttcggctcgg gagggagtgg aactaacagt ggcgggttat ctgccggcgg
     2881 cgcggcgagc ggtggtacga gcgccgcggg cggctctggg ggtgggttcg gcggacgcgg
     2941 cgggaagggc ggcgcttcga gcggcggaat gtctgcgggc ggcgcgatga gtagcggtgg
     3001 cgcttcgagc ggcggcgcga gcacgggcac cggcggcggg acgggtagtg ggtcggcgaa
     3061 gttcagtttc ttcgtgacga gtctcgaagc gatgcgacgg ctctcgaaga atcagaatgg
     3121 gtttggcggt gacttccgct acggagaagc agacgggctg gcgggcgcag acaagatctg
     3181 cactgaaatc gcggagtctt cgatgcccgg ttcgggagcg aagggttggc gcgcgttctt
     3241 gagtacgagc aaggtcaacg caatcgatcg agtgggaatg ggcccttggt acgatcgcga
     3301 ggggcgtttg gtggcgaccg gtgtgcaggg gctgctgcgg gatcgcccgg acggtgatgc
     3361 cgcggtggtg agcgacttac ccaacgagtc tggagttcca aaccgtgcag gtacgggggg
     3421 cggaatggat gataaccacg acaccgtgac gggcacgaat actcaaggca agtgggacgg
     3481 tggatccact tgctcggatt ggacgagcac gaccacttcg ggtggcccgc gcgtggggca
     3541 ctcttggccg gcaaacagcg gacgcaattg gatgcaggct cacaatgctc ctggatgcgc
     3601 tccgggagta aacctgtcat tgtcgagcgg ggccggcagc ggcaacggca tcggtaacgg
     3661 cggcggctac ggcggcatct actgctttgc tttgatgccc tgaatcggca cggtcgattg
     3721 gcgacgagtt catcccgtga aacgccagcg cgtgatgcgt ttagggcgtt cacgggacga
     3781 gctctcgcac gaccgagctt cacatgctct tgccgtcgaa ctgctgcacc ttcagctgat
     3841 tgacgtcgac gccgtcgagg cagcggacgt tgatggcgta catgggagtg ccatccgtgt
     3901 tcttgccgcg cccgaaggag cgaaccccgc agttcttgca aaacagatga tggatggtct
     3961 tggtcttgaa ctggtaatcg gacagagcgt cttccccgga cagcagcttg aacttgtcac
     4021 tcgtgacgaa gcccaagatg gcgcccgtgc gcgagcagat cgagcagtta caggagatca
     4081 gctggtctag cgtcatgtcg acgtcgtatt tgacggcgcc acagtgacag ctgccgtgat
     4141 aagtccgcgg ctccgatgcg ttgggggtcg aagtggtgga ttcggtttgg cccattggcg
     4201 tcccataccg cggctatgta cccgcggctt ggaaaagcgc gtcaattgcg ttggcctaga
     4261 gcagtgtgtg tcagcgttgc agcccggtgg cgccccgagc cgaaaaccgg ccggaagtac
     4321 tcaccggtgg atccgtgggc tgcgtggcgg gctcggtcgc aggcagagtg gtgggttcag
     4381 ggctggggcg gcgctgggtg ggggttacct gggcatgcgg taatcgtgtc gagccgtggc
     4441 ggtccacgtc ggtgcagacg tcattcaaat cgcaaaccaa gccgtaggtg gcgttgagcc
     4501 cacgccagga gcgaatctgg ggcatccact cgtcgtcttc gatggcggcg acgatcagcc
     4561 gaatccgcgt tggagttgcg atggactcca gcccttccgc atctctggcg gcggccgcca
     4621 acaggtcgct cgcggtgcga accccgttgg ccttgaggcg acccagcagc gcgcccccag
     4681 actcgctctc ggaggcatct tcgcgggtat gcagataaag gatggcttgg tctaccagat
     4741 cgaccaaacg tgaggcgggg atccgcgtgc ggagcagcaa gtccgtgagg ttgtggtggg
     4801 ctaggttttc gacattttcc acaccctcct ctagcagccg cgcggtgtca tagagggtga
     4861 tcccctctag cacgttgagc ggatcgcgat tcttcaggga cggaacgatc gctcccaccc
     4921 agcgattctt caggagatcg tgcaggaagg cgacgccagt atccggcacg atgccaacga
     4981 agaatacgag taacagcaag tgccacggag attgtgcgag cgcctctcca gccttgtccg
     5041 tggccagcgg cactgggggc gctcccaatt ccggataggt ctgactgatg acccacgcga
     5101 agatcgaagc cacgatgatg cgaacggcga tgtggctata tgccttgggc ccgagatcgg
     5161 cgcgcacgta gcgcctaaac accatgttga gcgagaagaa gtaggccccg aagaacgcaa
     5221 aagtgaaggg ctcccggttg gggatcagga acttgtgcac ctccaccggc gtggcgatgt
     5281 tcgtcccgaa cacctgcgcc atcacgtgca tcgacggcaa ggtcgccagc acccaaccga
     5341 gcgagagtaa gaacgtgctc gtgatgaccg gcacgaagaa caggggccct ttgcgcgcgc
     5401 tgagcgcttc atcgaggtga gctgcatact tcgtgtcgac ctcctccagg ttgcgcagcg
     5461 acggatccag gcgcagcagg tctcggcgat attgctcctt ggctacgtcg cgtcggagct
     5521 cggagaagag aaagaacaac agcgcaggta gcgtggccgc actgcctacg aaggtggcgt
     5581 acagcacgag cagcgccgtc ccgcgcagcg ctgcgttcga gctggacgcg gagagcatgt
     5641 gttggatcag cgcggagatg ctgccttgtg ggttgcactg taaccacgcg gccaacgcgg
     5701 gtagcccgaa ccccgtcagc atgaccgtca gaattcccga ggtgcggaat atgaagtcgc
     5761 gcgcgtcccc gaagtcgccg atgctctgca atcctgggat gtactggcga agtagtggta
     5821 cggcccacaa gaacgcggtc agcatggtgg tcaggaccag cgtcagccac gccagctgat
     5881 gcaacgagaa accgttcggg gccgtggcat aaacgaccaa gcccgaggcc aacgccaccg
     5941 gcagcgccgc caacaagaac aggtcgaggc ggggccggtc ccgttccagg ggtcgctcga
     6001 cgctcagaaa ccgcagcgcg atccagggat cgacgtcgag ttcacgcacc gcgctggggg
     6061 tggtcgacat gcgtgcgaac ggcgccagaa ttttaggcgg accattcgag cggtgcgcaa
     6121 tgcggatgcg ccgtgccgtc ttgttcacga gctccaagac gttctctcga gaccctgagt
     6181 cggattctga cgaaggctgg cgtggcatgt ttggagccca ctcgtatgcc gagccgcggg
     6241 ttcgcctcga cgccccaggg ccgagctacg gtaaagttcc ttcacgatac ggagtcgggc
     6301 cgtgagtggc catggtcatt cctcggatct gggcccaccg acgccctgtc gccctccagc
     6361 ggcgcggcgc attgccctca ggggcgctgg gctcgagctg cccaatcacc ctgagttcga
     6421 tagcgcttcg agttcgagct cgagcgcctc ccactccgcc atccagctcg cgatctgggc
     6481 ctcgagttgc ttctcttcgt tttcgagctc tgcgatcttg gacggcgcag tctgctcgta
     6541 gaaggtcggg tccccccact gggcttgaat agccgctttg cgcgcctcgg ccgactcgat
     6601 cgccgcgagc acgcggtcac gctcgttcgg cagttgcttc aggcggttct ttcgcttctt
     6661 ttgctcttcc caggagagcg cgctgctggt cgccttctcg gcctgggcct tgctcttttc
     6721 ctgcttgtct cgcagtgcca ccgcgttgga gtcgaggtgg tcgtccccgg actgggccag
     6781 gtattcggcg taagtgccat tgaaggcgcg gaagccgccc ggcgtcacct ctaggatgcg
     6841 cgtcgcgagc gcggccacga aagcgcgatc atgggatacg aacaacaccg tgccttcgaa
     6901 ggtttgcaag gcccggatca gggcctgaat agactcgata tcgaggtggt tggtgggctc
     6961 gtcgagcacc agcaagttgg gcttttggac catgatccgg cagaacacga gccgtgccgc
     7021 ttctccaccg gaaagcgagc ccaccttctt ctctacgtct tgcccggtga acagcaggtt
     7081 gccgagtctt ccgcgcacgt agctcgtagt ctcttgtggg atcgtatccc aaatcacatc
     7141 gagcacgcgc gcgtccggat cgctcaagag ctcatgatga tcctgcgcga agtacccgag
     7201 gcgagcttcg tagcctagct gcgttttccc ggcgtcggcc tcgagcttgc cagtgacgat
     7261 cttcaataac gtcgatttgc cgagtccgtt cgggccaatc accgcgaggc gttcaccgcg
     7321 gcgtagggtg agagagacgt ttcggagtac ggccttctcg ccgtaagatt tggagatgcc
     7381 ttgcatctcg agcacgtctt taccgctcgg tcgctcttgc tcgaaatgga agtggggagc
     7441 gcgccgggtg ctctgctcga gctcgtcgac ctcgatgcgc tcgagttgct tgaggcgact
     7501 ctgggcctgt cgggccttgg tggccttggc cccgaaacgc tcgatccagg ctttcttttc
     7561 cgcgatcgcg gcctcggcgc gcgcgatctc ggccgccttt cgggcgtggg tctcggcttt
     7621 ggatttctcg aacgcggagt agttgccagg atacaacgta accgtctgat aatcgacgtc
     7681 caggatgtga gtggccacgt tgtccaggaa gcgttgatcg tgggagatca ccaccgcaca
     7741 accgcgatag cccgccaaga acttctccag ccagcgaatc gacaggatat ccaagtgatt
     7801 ggtgggctcg tccaagagca agatgtcagg gccaccaatc agtacctgcg cgagcagtac
     7861 gcgcaacttg aaacctccgg acaaactcca gagtggcttc gaatgctggg gtaccggaat
     7921 accaaggcct tcgagcacgt ggctcgcgcg ggccttcaag gtatagccgt cgagcgaagc
     7981 aatgcggtct tcgagctcgg ctacgcgttc gggctgcgcg ccgctctcga tcagacgggc
     8041 ttgctcggca agggcgctgg tgacgagcgt gtcaccggac atcgccacgt cgagaatggc
     8101 ctcggagtcg tttgcgaact gatcttggcg caacacgccc aggcgcgcct gtttgggcaa
     8161 agtgacctgg ccatcgctgg ccggctcgtc gccggacaag atccgcagca aggtcgtctt
     8221 gccggatcca ttggcgccca ccaggccgta ccgtgcgccc gcgagcagct cgagcgaggt
     8281 cccctcgaac agggtgcgtg cgccgtagga tttgccgaga ttgacgagac cgatcatgag
     8341 ccggccccct agatagcagg gccgggcgag gcgcgcccgc gcgaggcggg ttcgtcagaa
     8401 gagctcgaga tcatcgagct cgatctggcc gtcatcggag ctgccttcgg gcaggaaccc
     8461 ggcgctgttg cgcccttcgc aggagggatt cagggcgagc ccgatcatcg ttccgagcac
     8521 ctcgtggggg tcgtcatggg gatccaggcc ttgatcgacg gcccacgact gcaccttggc
     8581 gtcggcgaaa tgcacctcca ccagtcgaga ttggtcactc accgggatat cccagccgaa
     8641 gcgcactccg gcgctcgtgc cggtctggcg tgggctctcc aaatccagtc gaagcgtgcg
     8701 ggcgcgatcg gcgcgcactc gaagccggac gccagtcaga gcgttcgcgt cgatttgggc
     8761 gccgcggaac gggatgctga agtagatcca ctggtcccac ggcttggttt cattgcggta
     8821 gctgaactcg agccgcaggt ctcgcgcgcc tgccagcggg tccgtgcgat tcaacgtgta
     8881 agtgacgcgg ctattctgat tgaacatcaa ggtcgcgccg agcaggagct cgaactcttc
     8941 gacgttctcg aagtcgttca gagcgcgggg ctcgaacgcg aacggaacgg gttgccgacc
     9001 ttccatcaga ctctgcatcc gacctcgaat caaaggaatg cgggtcttga ggtgctgatg
     9061 cgcggcctcg aaggtttccg gactggggcc gtgcggatcg gactccgccg ccgctcggat
     9121 caactcggcc tgttgatcga tgcgctccaa caagggcttc tcggcgaggg gcccgtccaa
     9181 gagctcttgg actgcgctgc ggtattccgt gagatccgag gcgagcgcct ggaacaggat
     9241 gttgcaaccg ggtgcgtaca caatgccgcc tccgttccaa accgggtagt gtagcgagca
     9301 atcggcgggt ttctctgtcc agtggggcac cgcgccgaag gtgccgaact cgaatgtgct
     9361 gtctagatcc caggggatga accacagctg cggacgtgtc tcttccttat agagatagaa
     9421 attgtgaatc aacgcatacg atgagttctc gtccgtgtag acggccgtca cgccatcgac
     9481 gttgagaatt gcgtcgtcca ccgccatgta gcggtacaaa ttggggagat cgacgaactc
     9541 ggctagcttg ctgcgggctt ccgccgcgga gctcgcctga ttcaacgccg cgcccaagct
     9601 caggggtact tcgtgggtgg cggtctcctc gttcgtctcc agatggtcgg cgtagtattc
     9661 gggatcggtc gacgtaggcc acgcctcctt gtagagatcg ccatccccgt tcttggggaa
     9721 acgatcgtcg gtgaagcgcc cgtctacctc ttccaccatg ctgaagaggc cctgagtttc
     9781 tccgtttaca cgcagcgtgg caaagaagga ttgcggggcg accacgccca tgtcccggaa
     9841 caagcgataa ccaatgcgct cgcgcaaatg ggaaggatcc caaatcatgg agtggaaatt
     9901 caggaccttc atgccgaaga agcgttgatc ggccgtgtac ttgtcgaact tgaccttcat
     9961 gctgagcttc ggacacttct gattgccggc cgcgtcgaag cagttcagca aagtgccgta
    10021 ggagcctttg aagcgaagcc ccacgttgcc aacggcgcgg ccctcgtagc aggccttcgc
    10081 ttccacgtac ttttcgtcgc gtgccgtacg cttcagctca ctccaggcgt cgggcggcag
    10141 gaccaagtcg aaaacgggga cgtggtccga ttcgaacagc tcgctgcgtt cgcgcttcgc
    10201 gagcgaggca tccggcgccc cgcattcggt cgccccggtc gtgcttccgc ccgtaccggg
    10261 gagagcgccg cttccccccg acccaccggt aattgcagcc gagcctccgc tcgtcgttcc
    10321 tccactagct ccggcgggag atcgcgtgcc gtcatcgcac gcggtcccgc tagccagcac
    10381 gagccagccg aagcgcgctg cgaagtgtaa cgaggtaaag cgaggcgaaa ggcgaataga
    10441 ccgcatggtt gttcgatgca cctgcgtgtc tcatgtcgaa cacgacccat gcagatcgaa
    10501 aaatcgtaca acgcgcatgc ttcccggtcc gatccccgag ttggaccgag ctggctgagc
    10561 cgatccagtc gagccggttt aggtgagccg agccagtgcg cgagctcgga gattgtagcg
    10621 cctaggatct atccccggcc ggggggtgtt acagctcggg agtggttgac tcgcaccagg
    10681 cgaatgcctg actcacccga gagcagcgtg tcccaccatt cttctccggc gtgccggtag
    10741 cgaatctgca cgcccactac ctcgcgccgg agcagccgag cgactgcttg gcgcagcgaa
    10801 caggtctcac ccgccgccag ctcggcgcgc cgcgtgccac cactcttcaa agttatatcg
    10861 agcagctcgg cggcgttctc cacgtctttg gcgagctgct cgacctcggc gcgtgtcagc
    10921 cacgcttcgt ggagcgtggg cagcggtgcg gtgttcggtt cgggttcggt actcaaagga
    10981 gattcggtac tcacggcagg catcgcgttg ggcaccttcg agctcagagc gcgggcgggc
    11041 tatccgcggg ttcgcgccgc gcgtggcatt gcagacagga ttgacggtcg gggtggggcg
    11101 tgcgcagtcc gttcggacct tgcggcccgt ggcagctggt acactggctg ctcatttgcg
    11161 taggatgggg gatggtcgga ggggcaccgg gccaagcgcg agttcccagg ccgtacgact
    11221 gcaggcctgc gaactcgttt accttggagg ggcctgcttc gggactgagt ttggggtcgg
    11281 ggacctgggc cgattcgaac tccggcgcga ccgcgtggca ctgtacgcag ttcgtataca
    11341 actcatggct cggcagggga gcggtgcgcc cagcgatacg cacgccatag gcatggcagc
    11401 tcacacagtc gttgagttcg cgctgcgcga tcgcgtgcgg aatcacgggg ggtgctcccg
    11461 cgtaggcgcg acgacgcgaa cgctcctcga ccgtcgcgat ccgctccgcg ggcgggacga
    11521 ggcgtgaatc gatcgggtcg ggggcagctc tcagcgcaac gaaagcattg ccatacatcg
    11581 cgccgttcgg gccgcggcgg acttcaccca gctcggagta gcttctggcg cgcggaaccg
    11641 cggcgttgac cgcggagccc gcggggggtg gccgccaggg ggccggcgaa gggcgcgcgg
    11701 tttctcccaa gaagcccacg gccgagagcg ttagcacggc gatgatgccg aaccacgtgg
    11761 ctcgtttggg gctgacgctt ggtcggtcga aggtcatggt tcacgtcttt cgagcgactc
    11821 ggcgcacgcg caccgcgcac ttcttgtagt ccggctgctt cgagaagggg tcgtatgcct
    11881 ccaacgtgac gtggttgatc aagagccgtt cgtcgaagaa gggaacgaac aagctccctc
    11941 gcggtgggct gccgcggccg tccagccata ccggcaactc gatctccccg cgtcgcgatt
    12001 ggacgatcac atgctccccg ttttgaatcc cgagctgggc cgcgtcatcg cggtgcatct
    12061 ccacgtaggc gttgggcatg gagcgacgca gcggtgggat ccgcatcgtc atactgccgc
    12121 tgtgccaatg ctcgatcaca cgaccggtgc agagccaaaa cggatactcg tcgtcggggc
    12181 gttcgggcgg cgcgacgtaa ggcctaaacc agacctgggc tcgcccgtct ttggtcggcg
    12241 aatgatagaa gtcgatatct tgtcccggct ttacgtaagg atcgtcgaaa gcggcgaacc
    12301 ggaacttggt ctcgcggaac acgccgtctt tctcgaccac gg
//
LOCUS       DASTKU010000365         4441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_737620, whole genome shotgun sequence.
ACCESSION   DASTKU010000365 DASTKU010000000
VERSION     DASTKU010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737620"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="VFQ61_12585"
     CDS             <1..1481
                     /locus_tag="VFQ61_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955340.1"
                     /translation="DSGRTYTKFSSIKNRASQSTYGYASISHKCLEPGYDLSLGRTIH
                     TYKLDVHTSQLVKQGKGGELVLLETGEFEETLRQLWYQHKGDPLIIENCRRRCAGGLY
                     SLHMEAVVAILYLGFAIDRPEFVRRRHLLSVTVSRVPCEPCARLLLGLLARFPELVLR
                     LKSSLLTADGTAGLKCLAAFQPPERLWFRFMPDDEVRRKFGPDEQSDAAEEVARWLHH
                     QAGFQPSTAAPHPSLLELATSNLDVGALQDGVRVFVESQLRTVTLPALWNLLSERVLI
                     QLLLVLAGGELDELLTKKVLTKKLTEKEMAYLRQCAGAAKKDESRPKVKRAAKPKDFA
                     NSLREAAKALATRAGDEATRKAIKKHVLDQIRISPSSWGQYQSKIKQATPAALDQEIE
                     TVKSLYRPFNVAGKSITLNWKEQGATRAELLTLAFPEQRALPEEREVELETGEKEEEG
                     EYWASDEAAPEARCLFCGVEVPAFTQFCNSSCEELCLQSGDW"
     gene            complement(1740..1955)
                     /locus_tag="VFQ61_12590"
     CDS             complement(1740..1955)
                     /locus_tag="VFQ61_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955341.1"
                     /translation="MNAITVAFANELEATRIKLNAACPGLTATDLNKFRGVRSVEQGA
                     RAAVRLALLDSNDPAGTFSNEDGPLPW"
     gene            2502..3302
                     /locus_tag="VFQ61_12595"
     CDS             2502..3302
                     /locus_tag="VFQ61_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955342.1"
                     /translation="MHDSVKAAWFEHSKKFEGRVPGMYCDILGLITCAQGNLIDPIGA
                     AEKLSWVLADGSPASIAQVRADWHTLKNDSARFAKLHFKNALAATKCRLTEETMDRLV
                     MAKLLENEAFLRKTFTKWDELPADAQLGIFSMAWACGPGFTAKFPNFTRLALAGDWVG
                     TVTKDATGGYPAKIREAGNPGVVPRNAANRLCFANAAAVLEQGLDVTELHWPDAVPPP
                     RAVAVAQDAKTASDVALDHFVTVELQRFLDDGPSAARDLRDFEEPHQA"
     gene            complement(3398..3841)
                     /locus_tag="VFQ61_12600"
     CDS             complement(3398..3841)
                     /locus_tag="VFQ61_12600"
                     /inference="COORDINATES: protein motif:HMM:NF040341.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HET9955343.1"
                     /translation="MFRWIGLSVLCAISSGCGSDADVGVKRQQWLKKAPSSYVVGTCT
                     TGFAIGACRLYAVQQGVVKAAEVKTPPDLMWKSSNDSQEPIARLFDSVERGQSGCKVS
                     VDFDETFSYPAKVYFDCGQEGSGEEVRCFQADAVDLEVCRAQTVR"
     gene            complement(3968..>4441)
                     /locus_tag="VFQ61_12605"
     CDS             complement(3968..>4441)
                     /locus_tag="VFQ61_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955344.1"
                     /translation="SGAFDAGFAVQGVVDGIYVLDSAGADRRTGDVVRVTGKLVDNFG
                     LLSIEPSSVAFLFRAPPLPPRASKTGSVGESSEGRLLHLRGTMVGPLVDDSPYGYKLT
                     LDDGSGPTQVFIYPGTNISVAGLVDGVTIDTQCFSSQFDTHYECDPPSPASFRIE"
BASE COUNT          857 a         1434 c         1417 g          733 t
ORIGIN      
        1 gggacagcgg ccgcacctat accaagttca gctcgatcaa gaaccgcgct tcccagagca
       61 cgtacggcta cgcgagcatc tcgcacaagt gcctcgagcc tgggtacgat ctgagcctcg
      121 gcaggacgat ccacacctac aagctcgacg tgcacaccag ccagctcgtc aagcaaggca
      181 agggcggcga gctggtgctg ctcgaaacgg gcgagtttga ggagactctc cggcagctct
      241 ggtatcagca caagggggat ccgctgatca tcgagaattg ccgcaggcgc tgcgcgggcg
      301 gtctctacag tctgcacatg gaggctgtcg tggccatcct ctatctgggc ttcgcgatcg
      361 atcggccgga gttcgtgcgc cggcggcacc tgttgtcggt gaccgtgagc cgagtcccct
      421 gcgagccctg tgcgcgcctc ctgctcgggc tcctcgcgcg ctttcccgag ctcgtgctgc
      481 gcctcaaatc gagcctgttg accgccgatg gcacggcagg gctcaaatgc ctggcggcct
      541 ttcagccgcc ggagcgtctc tggtttcgct tcatgcccga cgacgaggtg cggcgcaagt
      601 tcggcccgga cgagcagtcc gacgcagccg aggaagtggc gcgctggctg caccatcaag
      661 cagggttcca gccgagcact gcggcccccc acccgagctt gcttgagctc gcgaccagca
      721 acctcgacgt cggcgcgttg caggacgggg tgcgtgtctt cgtcgagagc cagctcagga
      781 ccgtgacgtt gccggcgctg tggaacctgc tgagtgagcg ggtgctgatc cagctgctgc
      841 tcgtcctggc cggcggtgag ctcgacgagc tgctgaccaa gaaggtgctg accaagaagc
      901 tgaccgagaa ggagatggcg tacctgcggc aatgcgctgg cgccgcgaag aaggacgagt
      961 cgcggccgaa ggtcaagcgc gccgccaagc cgaaggactt cgccaactcg cttagagagg
     1021 cggccaaggc gctcgcgacc cgcgccggcg acgaggcgac ccgcaaggcg atcaagaagc
     1081 acgtgctcga ccagatccgg atcagcccat cttcctgggg ccagtaccag agcaagatca
     1141 agcaagccac gcccgcggcg ctcgatcagg agatcgaaac cgtgaagagc ctgtatagac
     1201 ccttcaacgt cgcgggcaag agcatcacgc tgaactggaa agagcagggc gccacgcgcg
     1261 ccgagctgct gacgctggcg tttccggagc agcgcgcgct tccggaggag agggaagtgg
     1321 agctggaaac aggagagaaa gaggaggaag gggagtactg ggcgagcgac gaggcggcgc
     1381 cggaggcgcg ttgcctgttc tgcggcgtcg aggtgccggc gttcacacaa ttctgtaact
     1441 ccagctgtga ggagctgtgc ctccagtccg gagactggtg acgccgcgac ggggccgcgg
     1501 gggcgagcga gacacggccg agcgctcgag ctcgactcca acgctcaggg cggtcagcgc
     1561 gtgtgattca ccgccgggac cttgctggcg ctcgcgctca ggttgaccag gctgacgcag
     1621 gagacgggca agccgcgatc gactaaggtc tcgctcgcgc tcgcctcggt ccactcgagc
     1681 ccgcgtccga cctggcgcgg cgcggaggcg ggcgcgcagc tcggctcgac cagcgccgcc
     1741 taccagggca acggaccgtc ttcgttggag aacgtgccgg ctggatcgtt cgagtcgagc
     1801 agcgccagac gcacggccgc acgtgctccc tgttccacgc tgcgaacacc cctgaacttg
     1861 ttgagatcgg tcgcggtaag acccggacaa gctgcgttca atttgatgcg ggtagcctcg
     1921 agttcgttgg cgaacgccac ggtgatggcg ttcaaggcgg tcttcgaggg ctgtaggaga
     1981 cgctcttgat ggagcgaaac ggtgcgcgca cagaagttcg ccaggtcggc ttcgccaatg
     2041 ttcttacgat tcgaaggccg tgcgtcgctc gcctgcgagg cgctccgcga gttgctcggg
     2101 cgcttccgcg tcctcgtcgg gcggcgacgt gtcgacccac gggagcagat gttccggtgc
     2161 gaacgaggga aacgtgcagc agacaagatc tcgtgaaggg ttgcacgctt gcaacaagcc
     2221 cgagaaagtc agctccatgc gcagagcagc aacccgtgcg tccaaatcgt gtcaagcgtc
     2281 cggtgagaaa tctgcatgca tgatccggag tatcccgcag catcgccgca cacttagaac
     2341 gccgatcctc cctgaaagtt cccgaaacgc gcgcgtcgat atgttgctcg aaaaaaggtc
     2401 gcgaaccgcg aggcgccatg gacccgacaa tctgactatt gctttccgag gcgcccgacc
     2461 gaatagtgat ccgatctcga caacagagcg gctttcaact catgcacgat tcggtcaaag
     2521 cagcttggtt cgaacatagt aaaaagttcg agggacgcgt gcccggcatg tactgcgaca
     2581 ttctcggcct catcacgtgc gcgcagggca atttgatcga cccgattggg gcggccgaaa
     2641 agctctcctg ggtgctcgcc gatgggagtc ccgcgagcat cgcgcaagtg cgagccgact
     2701 ggcataccct gaagaacgac tccgcacgct tcgccaagct tcacttcaag aacgctctcg
     2761 ccgcgacgaa gtgccggcta accgaggaaa ccatggaccg tctggtcatg gccaagctcc
     2821 tcgagaacga agcctttctg cgaaagactt tcacgaaatg ggacgaactc cccgcagacg
     2881 cgcaactcgg gatcttctcc atggcgtggg cgtgcgggcc gggcttcacc gccaagttcc
     2941 cgaatttcac gcgcttggcg cttgcgggtg attgggtggg caccgtcacc aaagacgcga
     3001 ccggcggcta tcccgcgaag attcgcgagg ccggcaaccc cggagtcgtc ccgcgcaacg
     3061 ctgctaaccg gctctgcttc gcgaacgccg cagcggtgct cgaacaaggg ctcgacgtca
     3121 cggagctgca ctggcccgac gccgtgccgc ctccacgagc cgtcgccgtg gcgcaagacg
     3181 cgaagacggc ttcggacgtc gcactcgatc acttcgttac cgtcgaactc cagcggttcc
     3241 tcgacgacgg cccgagcgcc gcgcgcgacc tccgcgactt cgaagagccg catcaagctt
     3301 agagccagtc cacggcgctg cccgttgcct tcgaccggcc accggcttcc gccccctcgc
     3361 cggctgtgac gcacctgtcc ggagttgtgc cagcgactca gcgcaccgtc tgagcgcggc
     3421 acacctccag gtcgaccgcg tcggcttgaa aacagcgcac ctcttcaccg gagccctcct
     3481 gtccgcagtc gaagtagacc ttggcggggt aagaaaaggt ttcatcgaag tcgaccgaga
     3541 ccttgcagcc actctgtccg cgctcgaccg aatcgaacag gcgggctatc ggctcttgtg
     3601 agtcgttgct cgacttccac atcaggtccg gcggcgtctt cacctcggct gccttcacca
     3661 ccccctgctg caccgcgtag agccgacacg cgccgatcgc aaagcctgtc gtgcacgtgc
     3721 ccacgacgta actagaaggc gccttcttca gccattgttg acgcttaacc ccgacgtccg
     3781 cgtcgcttcc acatccgctc gaaatcgcgc aaagcacgct cagacctatc caccgaaaca
     3841 tgactccacc tttctagccc ctggggtccg ttacccgaag gaagcgtgac agcaaggcgc
     3901 gtgccggctc gttcacgatc gcgaaaaagc tcaggtggag gcagcaagct ggcgcgcgga
     3961 gccgccctta ctcgattcga aacgaagccg gcgagggagg atcgcactcg tagtgagtgt
     4021 cgaactggct gctgaagcac tgagtatcga tagtcacgcc gtccacgagg ccagccacgg
     4081 agatgttggt tcccggatag atgaagactt gcgttgggcc ggagccgtca tcgagcgtca
     4141 gcttgtagcc atacgggcta tcgtccacga gcgggcccac catggtgcct cgcaagtgca
     4201 agagccgccc ttcgctactt tcgccgaccg atccggtctt gcttgcgcgg ggcggaagcg
     4261 gcggcgcgcg aaacaggaac gcgacggacg atggctcgat cgagagaaga ccgaagttgt
     4321 cgaccaactt tccggtgacc cgcaccacgt cgccggttcg gcggtcggct cctgcagagt
     4381 cgagcacgta gatcccgtcg accacgcctt gtacggcgaa gccagcgtcg aaggcgcccg
     4441 a
//
LOCUS       DASTKU010000366         7346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_291802, whole genome shotgun sequence.
ACCESSION   DASTKU010000366 DASTKU010000000
VERSION     DASTKU010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291802"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..2407
                     /gene="fusA"
                     /locus_tag="VFQ61_12610"
     CDS             356..2407
                     /gene="fusA"
                     /locus_tag="VFQ61_12610"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET9955345.1"
                     /translation="MTYTTQGIRNIGLIGQTGGGKTLLLEALLLQAGVIRSKGSVQRG
                     NTVSDFGPQEKRLQHSLDVSVASFDWGAIHVNLLDTPGYPDFVGRALSALEAVETAAI
                     VVSATSGVDAVTRHMMDFARQRGLCRLLIVNKIDSREARTAEVLHELQEAFGRECLPL
                     NLPARGGTQVVDCFFKAKQEEATDFASVSETHTEIVDQVIDVDEALMAAYLEQGANLS
                     PEQLHDAFEKALRDGHLVPVCFVSAETGAGVPELLELIARLMPNPTEGNPPPFSRHGE
                     SGEVPVPVSADPLKHVVAHVFKVSIDPYVGKLGVLRVHQGTLNPGDQLFVGNARKAFK
                     LAHLYRLFGKDSTEMPRAVPGDICAISKVEELHFDAVLHDSHDEDHYQLKSVEFPPPM
                     LGLAIEPERRGDEQKLADALHKLAVEDPCVRIEHHPSMNETVLYAMGDLHLRVLLERM
                     TERHGVKVKTHPPRIPYRETITRTAEGHHRHKKQTGGAGQFGEVFLRVEPLERGAGFQ
                     FLDEVVGGAIPGQFIPAVEKGVRLVLEEGVVAGFPLQDVRVTVYDGKYHSVDSKEVAF
                     VAAGRKAFIDALRKAGPIVLEPVVRMEVTAPARAIGDVTGDLSSKRARISGTSAGSDS
                     ATIVALVPLAELSDYQSRLKSNTGGEGSYTMQLSHYDPVSTQRQQELVQAWKPRSENE
                     D"
     gene            2799..3941
                     /locus_tag="VFQ61_12615"
     CDS             2799..3941
                     /locus_tag="VFQ61_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955346.1"
                     /translation="MAIDRTGELNWPYGAEDVAGDGLAGFATAERKRDARTVYATTDN
                     ERLWVRLYVSDPGNPPNDLTAFLFVDADRNALSGGPARGAELDAKLPADPTPGGYEYA
                     LAFRGDGSAPRIWTWQPALASFQSTLLNASDADAEIGAEVDPIRLFDEVHGYLQVSLR
                     QALVALSGVCDGRLFLRTINESGAEGGQDVDVGAPFSCVADDLNQDRVPDVVVTSACQ
                     VNAQCPAGGVCAGGRCVVAAPCVDDGDCPADAACSSSEWCVARPGGSCSTNASCADLV
                     CRANRCDACSPSGSECGAGRVCALDGRCVGASGPSALPPALSPGEKVEGGALHCGLSR
                     PAGASTRGQALGIWFLVGLSIEGLRRWRRSSQMRRMNSDPRRGGRK"
     gene            3943..5388
                     /locus_tag="VFQ61_12620"
     CDS             3943..5388
                     /locus_tag="VFQ61_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9955347.1"
                     /translation="MRRTHWLCLGFGALALLRPARAQETNLDAERFKPAVTHDGFVTA
                     EGSAVRDREDPWEFGLYLNYARNTLVAVNGSGDVTRKFVAGRLGFDALASVTLAEPFA
                     IGLALPLFAAQTGDYSPSSAGLGDLRIVPKLRILDDRDGFGLSILAELRAPTHSGDFS
                     GGTRSVVFAPRVVADHRFAGGLRLGLNAGVLVREGTRFYNVHAASEFAYAGALGYRFG
                     GDAGKVELGAELAGAIGLVAASAAETPLEAFPYLKYNPTDEWELSAGPGIGLIAGYGV
                     PVVRGFLGVRFTPTSHDADHDGVPDRRDRCRNVAEDRDHVQDRDGCPEEDGDVDGIPD
                     SEDRCPNAKETINGFEDEDGCPDEGPARVIVEKGRIRILENIRFRKNSAEIDQDSHSI
                     LNQVALTMKANPSIKRVRVEGHTDETGTRDLNMQLSRERAESVRQYLVKRGVKPERLS
                     AAGYGPDRPLSRGSDADSLAKNRRVEFIVEQ"
     gene            complement(5501..5713)
                     /locus_tag="VFQ61_12625"
     CDS             complement(5501..5713)
                     /locus_tag="VFQ61_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955348.1"
                     /translation="MEQTYDYSGISVDRAIQEIQNDKRRKPAVVLAVIALGVIAAMGL
                     TYFSYRDVPAPLNPANARPHMVEPYH"
     gene            5917..6759
                     /locus_tag="VFQ61_12630"
     CDS             5917..6759
                     /locus_tag="VFQ61_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9955349.1"
                     /translation="MQRRKFGPLPVQVPVIGMGTWQLELDQPERSVAALRAGLDIGMT
                     HIDTAEMYGSGQAEALVGKAIEGRRAEVFLVSKVLPENASRKGTRQACERSLKQLRTD
                     YLDLYLLHWPSSHPLSETIAAFEELRSAGKIRAYGVSNFDVPDMEEALKLAGEGNLAC
                     NQVLYHLNERRIEHRVLPWCERNGVALVAYTPFGRAGFPPPGERGRVLARIAEKHRAT
                     PRQIALAFVTRNPLCFTIPKTSNIDHIRENAGAAGVSLDRSDLEALEASFPIGKLGST
                     LPTL"
     gene            complement(6798..>7346)
                     /locus_tag="VFQ61_12635"
     CDS             complement(6798..>7346)
                     /locus_tag="VFQ61_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955350.1"
                     /translation="GGAGGSDGTSETGGTAGASGTDGVSGSGGSESGGVGATGGSGTG
                     GAGGTVGVGGAGAMGGSGNGGTSGTAATSGAGQPGCTGCTPGETKPETQGCGPCGTGK
                     QTRTVTCTSSCQWTGDWGACIDPGTDKDHCSIVLDCVQLDHPRCVQKACTTAEAWAEC
                     QSESKTVCGSGSPAPSIANCGG"
BASE COUNT         1391 a         2223 c         2399 g         1333 t
ORIGIN      
        1 cgaggcacgt cgaccgtgct ccagaaggtt cgacagccga ctggtcgttc ccgcggtgag
       61 gtttgcaaag gcacgccttg ataccgaagg cgctggctga cttccgtcag gcagcgagga
      121 ccgcaccagg taccctggtc tgcattcggg tctgaacggt gtcagcgcgc ttgggtgctt
      181 cgtgaatcga tgcgccttct tcacacgatg cctcggagcg cgcgcgttgg acctggattt
      241 ctcgaaccct ctcgtgaaga gcgatctcac ttacttggcg cgaacaattg attggttctc
      301 cgggacagcg taacttcgaa tctcaaaagg catccgagcc aggcgagttc gcaccatgac
      361 ctacacgacc cagggaattc gtaacatcgg gctgatcgga cagacgggcg gagggaagac
      421 gttgttgctc gaggcgctgc tgctgcaagc cggcgtgatt cggagcaagg gcagcgtgca
      481 gcgcggtaac accgtgtcgg acttcggacc tcaggaaaaa cgcctccaac actcgctgga
      541 tgtgtcggtg gcgagcttcg attggggtgc gatccacgtg aacctgctgg atacgcccgg
      601 ttatcccgat ttcgtaggcc gagcgctgtc agccctcgag gcggtggaaa ccgcggcgat
      661 cgtggtgagc gcgacgagcg gagtggacgc ggtcactcgc cacatgatgg atttcgcgcg
      721 gcagcgaggg ttatgtcgac ttttgatcgt gaacaagatc gatagtcggg aagcgcgcac
      781 ggcagaggtt ttgcacgagc tgcaggaggc cttcggacgc gagtgcttac ccttgaattt
      841 gcccgcccgg ggcggcacgc aagtcgtcga ctgcttcttc aaggcgaagc aggaggaggc
      901 gaccgacttc gcgtcggttt ccgagactca caccgagatc gtcgaccaag tcatcgatgt
      961 cgacgaggcg ctgatggcgg cctacctcga acagggcgcg aacctttcgc ccgaacaact
     1021 gcacgatgct ttcgagaagg cgcttcgcga tggccacttg gtgcccgtct gcttcgtgtc
     1081 ggcagagacg ggcgccggcg tgcccgagtt gctggagctg attgcgaggc tgatgcccaa
     1141 tcccacggaa ggtaacccgc cccctttctc ccgccacggc gagagcgggg aggtgcccgt
     1201 accagtcagt gcggatccgc tgaagcacgt ggtcgcccat gtgttcaagg tcagcatcga
     1261 cccgtatgtc ggcaagctgg gcgtgctgcg cgtgcatcaa ggaaccctga atcccggaga
     1321 tcagttgttc gtgggaaacg cgcgcaaagc cttcaagctc gcgcacctct accgcttgtt
     1381 cggcaaagac tcgacggaga tgccgcgggc ggttccgggg gacatctgcg ccatctcgaa
     1441 ggtggaagag ctgcatttcg acgcggttct gcacgactcc cacgacgagg atcactatca
     1501 gctcaagtcc gtagagttcc cgcctcccat gctcgggctc gcgatcgagc cagagcgacg
     1561 cggggacgag cagaaactag cggacgccct gcacaagctc gcggtggaag atccgtgcgt
     1621 acgcatcgaa catcacccat ccatgaacga gactgtgctg tatgcgatgg gcgatctgca
     1681 cttgcgcgtg ctgctagagc ggatgacgga gcgtcatggc gtaaaggtca agacccaccc
     1741 accacgcatt ccctaccgag aaaccatcac gcgaaccgcc gagggtcacc atcgtcacaa
     1801 gaagcaaact ggcggcgcgg gtcagttcgg tgaagtgttc ttgcgagtgg agccgctcga
     1861 acgaggcgcg gggttccagt ttttggacga ggtggtcggg ggagcgatcc cgggccaatt
     1921 cattccggcg gtggaaaagg gagtgcgctt ggtactcgaa gagggagtgg tcgctggctt
     1981 cccgctgcaa gacgtgcgcg tgacggtgta cgacgggaag tatcactcag tggattccaa
     2041 agaggtggcg ttcgtcgcgg cggggcgcaa agccttcatc gatgcgctcc ggaaggcggg
     2101 gcccatcgtt ctggagccgg tggtgcgcat ggaagtgacg gcgccggcgc gcgccatcgg
     2161 tgatgtgacg ggcgatctct cgagcaagcg tgctcgcatc agtggcacga gcgcagggtc
     2221 ggattcggcc accatcgtcg cgctggtgcc gctggctgag ctaagcgact atcagtcgcg
     2281 cctcaagtcc aacaccggcg gcgaaggctc ttacacgatg cagctgagcc actacgaccc
     2341 agtctcgaca cagcggcagc aggagctcgt tcaagcctgg aaaccgcgca gcgagaacga
     2401 agactgagcg cctacggctc gattcacacg ttctgctcca atgtcgctca gcgtagccgg
     2461 gtgatattgc gcgggcaatg accacttggc tccgcactgg agatccgccc caagaggcaa
     2521 ttggcgcgct ggacgtcgac cgctattcaa gagcgaaaac cacgctgctc gggcgcgaag
     2581 gagtgtagcc tgcgctcacg atgcgcagac gctctgaatc acggcgctct gaatcacggc
     2641 aatccgaatc gcggcaatct gaatcacgcc gcaagacgca gattgattgg cgaggctgga
     2701 cggtagcatg ggcttgcata gcgggcgctc tatcgtggtc tccacgagcg gaagcggcct
     2761 ggcccggagc caggtcgcgg ccctcgctgc gacaaatcgt ggccatcgat cgaactggcg
     2821 agctcaactg gccttacggc gccgaggatg tagcgggtga tggcttggct gggttcgcga
     2881 ccgcggaacg caaacgcgac gcacgcacgg tctacgccac caccgacaac gagcgacttt
     2941 gggttcggct ctatgtctcg gatccgggca acccaccgaa cgatctgacc gcgttcttgt
     3001 tcgtggacgc ggatcgcaac gcgctcagcg gtggccctgc ccgcggcgcg gagctagatg
     3061 caaaattgcc agccgacccg actccgggcg gctatgagta cgcgctggcg ttccgtgggg
     3121 acgggagcgc gcctcgaatc tggacctggc aacccgcctt ggcgagcttc cagagcacgc
     3181 tcctcaacgc cagcgacgcg gatgcggaga tcggcgcgga agtcgatccg attcgcctgt
     3241 tcgacgaggt acacggctac ttgcaggtgt ccttacgcca agcgctcgtc gcgttgagcg
     3301 gcgtgtgtga tgggcgactg ttcctcagga cgatcaatga gtcgggcgcc gaaggcggcc
     3361 aggacgtgga cgtcggagcg ccgttctcct gtgtcgctga cgatctgaac caggatcggg
     3421 tcccggacgt cgtcgtgacc tccgcttgcc aggtgaacgc tcaatgcccc gcgggaggcg
     3481 tctgcgcggg cgggcgctgt gtggtagccg cgccgtgcgt ggatgacggc gattgccccg
     3541 ccgatgccgc ctgttcttca tccgaatggt gcgtagcccg ccccgggggc agttgcagca
     3601 cgaacgcgag ctgcgcagac ttggtctgcc gggccaaccg ctgcgacgct tgttcccctt
     3661 ccgggagcga gtgcggcgcg ggacgtgtat gtgcattgga cggccgctgc gtgggcgcga
     3721 gcgggccttc ggctctgcct cccgcgctgt cacccggaga aaaggtggaa ggtggcgcgc
     3781 tacactgcgg ccttagtcgc ccagccggcg cgagcactcg aggtcaggca ctagggatat
     3841 ggttcctcgt gggcctgagt atcgaggggc ttcgccgttg gcggcgaagc tcgcaaatga
     3901 gaagaatgaa ctcggatcca cgccgcggag gacggaagta acatgcgtcg cactcattgg
     3961 ctttgcctcg ggttcggggc gctcgcgttg ctacgtcccg cgcgggctca agagaccaat
     4021 ctcgacgcgg aacgcttcaa gcccgcggtg acacacgatg gcttcgtgac cgctgaaggc
     4081 tcggcggtgc gcgaccgcga ggacccctgg gagttcgggc tatacctcaa ctacgcacgc
     4141 aacaccttgg tagcggtgaa tggctccggg gatgtgacga gaaagttcgt cgccggccgc
     4201 ctagggttcg atgccttggc ttcagttacc ttggccgagc cgttcgcgat tggtctcgcg
     4261 ttaccgttat tcgccgcaca gaccggcgat tattcgcctt cctcggccgg tctcggggat
     4321 ttgcgcatcg tgccgaagct ccgcattctg gacgatcgcg acggcttcgg actgagtatc
     4381 ctggcagagc tgcgcgcgcc cacgcattca ggagacttct cgggagggac gaggagcgtg
     4441 gtatttgcac cgcgtgtggt cgccgatcac cggtttgccg gcggattgag gcttgggttg
     4501 aacgcgggag tgttggtgag agaaggcact cgattctaca atgttcacgc cgccagcgag
     4561 tttgcatacg ccggagcact cggctaccgc ttcggcggag acgccgggaa ggtggagctc
     4621 ggggccgagt tggccggagc gataggcttg gtcgccgcat cagctgcgga aacgcctctc
     4681 gaggcatttc cgtacctgaa gtacaatccg accgatgagt gggagctgag cgcgggcccc
     4741 gggattggct tgattgcagg gtacggcgtg ccggtggtgc gaggcttctt gggtgtgcgc
     4801 ttcacgccaa cttcgcacga cgccgatcac gacggcgtcc ccgaccgtcg cgatcgttgc
     4861 cgcaatgtgg cggaggaccg cgaccatgta caggatcgcg acggctgccc cgaagaggac
     4921 ggcgatgtcg acggaatccc ggacagcgaa gaccgttgcc ccaacgcgaa agagacgatc
     4981 aacggcttcg aagacgaaga cggctgcccc gacgagggcc cggcgcgcgt gatcgtcgaa
     5041 aaaggtcgca tccgcatctt ggagaacatt cggttccgca agaattcggc ggaaatcgac
     5101 caggattcac actcgatttt gaaccaggtg gcgctgacga tgaaagccaa ccccagcatc
     5161 aagcgagttc gcgtggaggg tcacaccgac gagacgggca ctcgagatct caacatgcag
     5221 ctgagtcgtg agcgcgccga gtcggtacga cagtacctgg tcaagcgagg tgtaaagccg
     5281 gagcggctga gcgcggccgg ttatggcccc gatcgtccac tctcgcgcgg tagtgatgcg
     5341 gacagcctcg ccaaaaaccg ccgagtcgag ttcatagtgg aacagtagct tgcggagcac
     5401 gcaccgacgc gcgcgatcgg aatatcgcgc gcgtcatcaa ggcacgcttc tcatgacccg
     5461 attaggcaac tgcaacacta caccgcaaca gctccgcatt tcagtgatac ggctcgacca
     5521 tatgtggccg cgcattggct ggattcagcg gcgccgggac gtctcgatag gaaaagtagg
     5581 tgagccccat agccgcaatg acgccgaggg cgatcaccgc cagaactacc gcgggtttac
     5641 ggcgcttatc gttctgaatt tcctgaatgg cgcgatctac ggatatgcct gaatagtcat
     5701 acgtctgttc catggcctct cctctctagg ctggtactcg aagcatacgg tgtgcccgag
     5761 aggggcatga cgcttgggtt cagccacggc acggggctga accgcagatt cgccacttgc
     5821 cttgtgcggc ggtgatcatt gccgcacacg cttgcccggc gcaggaatca aaacagaatg
     5881 atccacgaaa gccttgcgtg tggcacgcta ggcaccatgc agagacgcaa atttggcccg
     5941 ctgccggttc aagtgccggt aattggaatg ggaacttggc agttggagct ggatcagccc
     6001 gaacgcagcg tggccgcgtt gcgagcgggt ctcgacatcg gcatgacaca tatcgacacc
     6061 gccgaaatgt acggctcggg tcaagccgaa gcgctggtag ggaaagccat cgaagggcga
     6121 cgcgcggagg tcttcttggt tagcaaagtg ctgccggaga acgcgagtcg aaaaggcacg
     6181 cgccaagcct gcgagcgaag cttgaaacag ctgcgcacgg actacttgga tttatacctg
     6241 ctgcactggc cgagttccca cccgctctcg gagacgattg ccgccttcga ggagctacgc
     6301 agcgcaggaa agattcgagc gtatggggtc agtaacttcg acgtaccgga catggaagag
     6361 gcgctcaagc tggcgggcga gggcaatctc gcctgcaatc aggtgctcta tcacttgaac
     6421 gaacgccgca tcgagcaccg ggtattacct tggtgcgagc gaaatggagt cgcgctggtc
     6481 gcatatacgc ctttcgggcg cgctggtttt ccgccgccgg gcgaacgcgg ccgcgtgctg
     6541 gcgcgaatcg cggagaagca ccgagccacc ccgcgccaga ttgcgttggc cttcgttacc
     6601 cgtaatccgc tgtgcttcac gatcccgaag acgtccaaca tcgatcacat tcgtgagaat
     6661 gcgggggctg cgggcgtgag cttggaccga agtgatttgg aagctctcga ggcgtcattc
     6721 ccaattggaa agctcggttc gacacttccg acgctctgaa gagcagagcg gcgttcgaac
     6781 gtcagtagtc cagcgcacta gccgccgcaa ttcgcgatgc tcggggcagg agagccggag
     6841 ccgcagacgg tcttcgactc ggactggcac tctgcccacg cctcggccgt cgtgcacgcc
     6901 ttctgcacgc agcgagggtg atctagctga acacagtcca ggacgataga gcagtgatcc
     6961 ttgtccgtcc cgggatcgat gcacgcgccc caatcacctg tccattggca gctgctggtg
     7021 caggtgaccg tacgagtttg cttccccgtc ccgcatggcc cgcagccttg cgtctcgggc
     7081 ttcgtttcgc ctggcgtaca gcccgtgcat ccaggttgcc cagcaccact ggttgccgcc
     7141 gtgccgctgg ttcctccgtt accggagccg cccatggcgc cagctccacc aacacccacc
     7201 gtaccaccgg cgccgccggt cccgctaccc ccggtcgcgc caacgccacc gctttcgctc
     7261 ccgccgcttc cgctaacgcc gtcggtccca ctcgcgccgg cggttccccc ggtttcggat
     7321 gtcccatcac tgccgcccgc gccgcc
//
LOCUS       DASTKU010000367         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_291984, whole genome shotgun sequence.
ACCESSION   DASTKU010000367 DASTKU010000000
VERSION     DASTKU010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291984"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFQ61_12640"
     CDS             <1..189
                     /locus_tag="VFQ61_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET9955351.1"
                     /translation="EAAGMRVDYVELATPEELEPISDGSTVGERALLALAAFMGKTRL
                     IDNVVLGEDPPPIQETEG"
     gene            201..863
                     /gene="tmk"
                     /locus_tag="VFQ61_12645"
     CDS             201..863
                     /gene="tmk"
                     /locus_tag="VFQ61_12645"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET9955352.1"
                     /translation="MSETSRSQTFKVPSRRPGRFIVVEGIDGAGTTTQARHVAERLRG
                     LGKDVIATFEPSEGPVGVMIRAALEGRGRGFDWATLALLFAADRLDHLAANVQPHILS
                     GTWVISDRYTLSSLVYQSVTAPPEVPDALEWVRGLNRSARLPDLTLVLDVPSAVAHER
                     REKRGAPEELFDAASLQERLAQAYARAQQLAPEQRVVHVSGLGAEHEITDRLVAAILA
                     AL"
     gene            complement(1062..1928)
                     /locus_tag="VFQ61_12650"
     CDS             complement(1062..1928)
                     /locus_tag="VFQ61_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955353.1"
                     /translation="MMLRTMAPIVGALLLAMGAGACQVGEAPEDSASFDAALCSNSDG
                     TNYMLASLAAATAKELRRWQPGVDFREVYKCNYVYTGPSCTTVIELTPAGKSRCADGQ
                     CKNVQAILDFQKREANLSVKFPGGVVFRNDTFVNRLVANFRRQITCNSQPDNHNDSNC
                     PSEAHDLAFSYSAPGGCETDYWFHAYKAGTQQPLTYPRQLKNQLLMFGYLDGWQSNPY
                     LAFDVQGDDVKIDPGPGTIADDPSLTGACPVLGIDGRFSFWDISGQCCMYQGRAGRFW
                     RSAFSYYFYLCR"
     gene            complement(2148..3026)
                     /locus_tag="VFQ61_12655"
     CDS             complement(2148..3026)
                     /locus_tag="VFQ61_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HET9955354.1"
                     /translation="MSSTHLARCLPVLLLAACSGAPEMTSEEASDSSQENIVRGTVDA
                     GHPQVMALLVPRADGSQEFCTATLYARRTLLTAAHCLVGAELVLAYHGNDFMNAFDQL
                     GQDPKTWTNWAQAVDWKIHPKWDPKTWSADIAVVHIDRDPPFPPLPLATKEIGKSYVG
                     SMAQLVGYGGLASEADNATPIDAFIKRFGTTKYQGTPAASPLPPNPHPGLGIRAIREQ
                     LMQFDGKAPYVNACFGDSGGPALMSFAGKYQVVGVATWTGDYCRDFSYYTRVFDFAPF
                     LTEQALPKNATPPLPL"
BASE COUNT          601 a          981 c         1019 g          592 t
ORIGIN      
        1 gaggctgcag gcatgcgcgt ggattacgtg gagctggcga cgccggaaga actcgaaccg
       61 atttctgatg ggagcactgt gggcgagcgg gctttgctcg cgctggccgc gttcatgggc
      121 aaaacccggt tgatcgacaa cgtcgtactc ggtgaagacc cgccaccgat ccaagaaacg
      181 gaaggatgag cgataggagc atgagcgaaa caagccggtc ccaaactttc aaagtgccgt
      241 ctcggaggcc tggtcgcttc atcgtcgtcg agggcatcga tggggcggga accacgacgc
      301 aggcgcgtca cgtcgccgaa cgcctgcgtg gcctcggcaa ggacgtgatc gcgacattcg
      361 agcccagcga agggccagtc ggagtgatga tccgcgccgc gctcgagggt cgcggacgcg
      421 ggttcgactg ggccaccttg gccctactct tcgccgcgga ccggctggat cacctggccg
      481 ccaacgtaca accccacatc ctgtcgggca cgtgggtgat cagcgatcgc tacacgctct
      541 cgagcttggt ataccaatcc gtgaccgcgc cgcccgaggt ccccgacgct ctcgagtggg
      601 tccgcggttt gaaccgctcg gcgcgcttgc ctgatctcac gctcgtcttg gacgtgccct
      661 ccgcggtggc gcacgagcgg cgcgagaagc gaggtgcgcc agaggagctg ttcgatgccg
      721 ctagcctgca ggagcgtctg gctcaagcgt atgcccgcgc tcagcagctc gcgcccgagc
      781 aacgtgtggt gcacgtctcc ggtctcggtg cagagcacga gatcacggat cgcctggtcg
      841 ccgcgattct ggcggccttg tgagaagagt cgcgctcgca ggctagtccc gcccatcgcc
      901 cgcgctccgg ccaccagaag cccagaaccg cacgtcggtt ctgggcttct ttgcagttgc
      961 gacgccaata ggaacctacc agggagagct acgtgcagcg aacttcacga aggcggcgcc
     1021 ccaattcgga gaactagatt gaacgctggc ggctcgggcg ctcagcggca caagtagaag
     1081 tagtagctga acgccgaccg ccaaaagcgg cccgctcgac cctggtacat gcagcactgg
     1141 ccagagatat cccagaagct gaagcggcca tcgatcccga gcaccggaca ggcgccggtg
     1201 aggctcgggt catccgcgat ggtgccggga ccagggtcga tcttgacgtc atcaccctgc
     1261 acgtcgaacg ccaaataggg gttcgactgc cagccatcga gatagccgaa catcagcagc
     1321 tggttcttta gctgccgagg gtacgtcagc ggctgttgcg tgcccgcctt gtacgcgtgg
     1381 aaccaatagt ccgtctcgca gccgcccggc gcggagtagg agaaggccag atcatgggct
     1441 tcgctcgggc aattggaatc gttgtgattg tcgggctggc tgttgcacgt gatctgccgc
     1501 cggaagttcg cgaccagccg gttcacgaac gtatcgttcc tgaagaccac gccaccgggg
     1561 aacttgacgc tcagattcgc ctcgcgcttc tggaagtcca agatcgcctg gacgttcttg
     1621 cattgcccat cagcgcagcg gctcttaccc gcgggagtga gctctataac cgtcgtgcag
     1681 ctcggccccg tgtagacgta gttgcacttg tagacttcac ggaagtccac ccccggttgc
     1741 cagcgccgca gctccttggc cgtcgcggcc gcgagcgaag cgagcatgta gttggtacca
     1801 tccgagttcg aacatagagc tgcgtcgaag gacgcgctgt cttccggtgc ctcgccgacc
     1861 tgacaggcgc cagcccccat tgcgaggagc agggcgccga cgatcggagc catggttcgt
     1921 agcatcatga gttctccttt tgccgaatcg cgcagcttca cacccgaggg ttagctgcgt
     1981 tgacttactc tgcacatcgc gtgccgccgc gagcgagttc gtatccgcac tcgactcgct
     2041 cggtgattgc caatggccca caaaacaaga cgaccgagct gcgtccacaa ctcggtcgtc
     2101 tcacggctga gggcttgcca ctcagcgcaa tcgatgggtg gttagaacta gagcggcagc
     2161 ggcggagtcg cgttcttggg caaggcctgc tccgtcagga acggagcaaa gtcgaacacg
     2221 cgcgtatagt aggaaaaatc acggcagtag tcccccgtcc aagtggcgac acccaccact
     2281 tgatacttgc ccgcgaacga catcagcgcg ggcccgcccg agtctccgaa gcaagcgttg
     2341 acgtacggcg ccttgccgtc gaattgcatc aactgctcgc gaatcgcgcg gataccgagg
     2401 ccgggatgcg gattgggtgg taacgggctc gcggcggggg tgccttgata tttggtggtt
     2461 ccaaagcgct tgatgaaggc gtcgatcggg gtcgcgttgt cggcctccga cgcaagccct
     2521 ccatagccta cgagttgagc catgctgccc acgtaagatt tgccgatctc tttcgtcgcc
     2581 aaaggcagtg gtgggaaggg cggatcacgg tcgatgtgga cgacggcgat atccgcgctc
     2641 cacgttttgg ggtcccactt cgggtggatc ttccaatcga cagcttgggc ccagttggtc
     2701 caggtcttgg gatcttgtcc caactggtcg aaggcattca tgaagtcatt gccgtgataa
     2761 gccagcacga gttctgcgcc gaccaggcag tgcgccgccg tcaataacgt gcgtcgggcg
     2821 tagagcgtcg ccgtgcagaa ctcctgtgat ccgtctgccc gaggcaccaa cagggccatc
     2881 acctgcggat gcccggcatc cacggtgccg cgcacgatgt tctcctgcga tgaatcactg
     2941 gcttcctcgg aggtcatttc cggtgcacca gagcacgccg ctagaagcag gacgggcaga
     3001 cagcgggcga gatgagttga cgacattcgg gatctttctc ctggacgcaa ctcggccgtg
     3061 aacgcgcggg gtgcgcgagt ggtggccgaa tttcctactc gcagtggcga gttgcacgaa
     3121 gcgatccacg gcttacaggc tcggcgaatc ccgcctgttt cgagttcgtt cttaagcgtt
     3181 cagggtgaac tgc
//
LOCUS       DASTKU010000368        10165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_516253, whole genome shotgun sequence.
ACCESSION   DASTKU010000368 DASTKU010000000
VERSION     DASTKU010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10165
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516253"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2408
                     /locus_tag="VFQ61_12660"
     CDS             <1..2408
                     /locus_tag="VFQ61_12660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9955355.1"
                     /translation="GSAELARATEIGILLEDTQRIEAQFGPYKSDPDVLAIVVTDAHG
                     APLVLHGSLPQPLTEIFSGPEGGLSRLPNAYVSWRRAMVEGIEVGRVAVAASNLRLDE
                     GSRLERRILLGAFIGGLLAFAAALGFVAFYVRPVIRVTQAAFARLEKTTELALQAARM
                     KSEFLANMSHEIRTPMNGVLGMIELLNGTTLEAKQRRYTQTLQASAAALMTVLNDILD
                     FSKMEAGKLRLQPDATELRTVVEEVAELFGARAHLRNIELACHIDRELPRFVEVDSDR
                     LRQILSNLIGNAVKFTDHGQIVIRVKLLGPAGDSKSKRRVDVRFEVEDSGVGIDAATR
                     GRLFEAFSQADGSFTREHGGTGLGLAICSKLVELMRGSIGVESEVGCGSTFWFVLPLE
                     PVEMEQASERGLHLRRARTLIVDDNETNRLILEELLSSWGFPNQSVPDVDSALRALTR
                     AQRSGEAFGLVISDMHMPEVDGLTLARHVRGSEHGDLPLILLTSQSEELPPEELDLLD
                     GILQKPVRAEQLSSVIRRALHSGTARHSSPGESAESRTQKLAHSKILVVEDNPINQEV
                     ICEMLRELGIGAVVADHGAHALELLATHSFPVVLMDCQMPVMDGYTATREIRRRENGA
                     SRMPIIAVTAHAMQSERDKVQAAGMNDYISKPITRRALAAALQRWWPELAESAPISIV
                     NVPRTVLPQPSSLDRAVRRSAVVIRSFVRHAPSQIEAIQSALREGDAAALKQAAHLLK
                     GGCLAVGVPRMASLCARLEVNPVDRLELCSQLEREYAKVQARLEALLVSEPQSVEASR
                     YKR"
     gene            2723..3199
                     /locus_tag="VFQ61_12665"
     CDS             2723..3199
                     /locus_tag="VFQ61_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955356.1"
                     /translation="MKCPDDQQTGPLGTCVCKTGYVLGAAGCEPIPAGLGVDCSASGM
                     CGAEPYSYCMETDRGSYCTSKGCKTSADCAFGYACDVPSGVCLRPPVGLYMTCQSDKD
                     CADTEATYCDVFFSHSCLVQGCTVSPNNCFEGSECCDLSTFGVAQPLCIPAGSCTT"
     gene            3196..4749
                     /locus_tag="VFQ61_12670"
     CDS             3196..4749
                     /locus_tag="VFQ61_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955357.1"
                     /translation="MRTRLISNLVLGALVAAYLGASPAATAQTAAASPGASLPTSPVA
                     PAPAAAAAPESELEGPIPAAATAPALVAETPASAPAAPVEVGAAATSAADAADASASS
                     VPAAPASTPTAEEPLDLSQFTQSDTSSIGSDEGKLNLYGFSDFSYFLPLRRGIGITPY
                     PTFYVGNLNLYMATDLGADFRSMVEVRFSYLPHGATPLAADGFTALPRVNNTAPDYTD
                     LGRPLRWGGISIQRAWLEKTFHPLATVRVGHWLTPYGIWNVDHGSPVIISVRRPYIVG
                     ESFFPQSQTGIELYGSTAFGPAQVGYHLTLSNGRGPLDTYQDLDSNKAVGVRLWGKYD
                     SDFGTFTLGTSMYRGQYADRTATYVMNESGAFDSKWLPTVKYDELSLAADFKWEWKGA
                     LFQAEGILNDVTYKTEEKLRPVVTSAYGGPNGYTPDSRRSGAYGLIGYRTDFLGTMPF
                     FGGEFYHVGMHSFTPDAAAIWTGLNVRPTPRVVLKAVYSRSWFTNTSPFGASFESFEA
                     LDFQAAWGF"
     gene            4751..5242
                     /locus_tag="VFQ61_12675"
     CDS             4751..5242
                     /locus_tag="VFQ61_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955358.1"
                     /translation="MMTTRRQLFPMTLGLLAGLGLNRSVFAKDAPSRLAVVVAKSSPL
                     QSIGFHELKRLYMGETVNGPEGMRLVALNHAPLSTERVQFDKSVLGMSPEEVARFWID
                     RKIRGQSGAPKTVEPLDLLQRVVAQLRGAVGYCRLEDVKGELRVIKVDGRLPNEGGYP
                     VST"
     gene            5274..5765
                     /locus_tag="VFQ61_12680"
     CDS             5274..5765
                     /locus_tag="VFQ61_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955359.1"
                     /translation="MIGRRALFPLSAFVLAELGFAPSCWAAGEGGSRLAIVVAKGSPL
                     QSIGFHELKRLYMGETVNGPEGLRLLALNQSSVSAERMRFDRGVLGMTPEQAARYWID
                     RKIRGQPGAPKAVDMVEVLQRAVAQLRGAVGYCRLDEVRDGLRVLKIEGKLPTDAGYP
                     VSG"
     gene            5846..6856
                     /locus_tag="VFQ61_12685"
     CDS             5846..6856
                     /locus_tag="VFQ61_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9955360.1"
                     /translation="MTEALSLGVDLGGTKVEAVLLRRTPAGPGFEILTRERVATGSER
                     GYEAILTTTAELVRNVARSSGRPLLELAVGVGMPGSVSRTTGLVKNSNTVCLNGRPFR
                     EDLSRALGCPVAFENDANCFALAEAYLGAGRAHRKGIVFGVILGSGVGGGIVIDGRVW
                     PGPQNLAGEWGHHAVGPWARVEMLAGAAPSAAGFAACGLSPRARCYCGKMGCLELYAS
                     GVNVEREYERRTGRRRILKELVASRIDDVDAASVVDEMIEAIGRGLANVIDILDPSAI
                     VLGGGVSNLDILYGEGVARVARYLFNDELRTPILKHELGDSAGVLGAALLHTDEYTHA
                     SE"
     gene            complement(7021..7491)
                     /locus_tag="VFQ61_12690"
     CDS             complement(7021..7491)
                     /locus_tag="VFQ61_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9955361.1"
                     /translation="MKIAEIMTRDVSSCTPHDTLATAAQIMWENDCGAVPVVDRDGKL
                     VGIITDRDLAMAAQLQGVALRESSVASAMARDVKCCAPQDTPATVQALMQQHKIRRVP
                     VVDAERRLIGIISLGDLAYVMSSTQTLGGDGMTWTALAHTLAAVSQPRISRYRR"
     gene            7741..8553
                     /locus_tag="VFQ61_12695"
     CDS             7741..8553
                     /locus_tag="VFQ61_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9955362.1"
                     /translation="MDFAHYAQRPDAQLALLEGSVLIAQDEYPGFQLANAEAAIEELA
                     LPLESLQLSRMSARAQARALSEHLYVACGFRGDVETYHAPENSYLNRVLERRQGIPIS
                     LAVVYMEVARRLSVSARGVGFPGHFLVRLDTEHDSAIVDPFFGGEILDSARLEQLLGN
                     MRPRLKLHQDMLEPASVRAIIARMLTNLRAIYSARADAGRLLVVLDHLSDLLPDAVDV
                     VRDRGFLHARMGANAAAIADLQCYVDVLPHADDAEQIRAAIERMSGSSSTQN"
     gene            complement(8649..8960)
                     /locus_tag="VFQ61_12700"
     CDS             complement(8649..8960)
                     /locus_tag="VFQ61_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955363.1"
                     /translation="MAERGFARNLVSLLLIVGTGVGLYNVYSDNSELKAQAARLACSE
                     RPCDHELQRESRSPISQSFTFRVELKLPKDRRRATVDVDCRRAFWLVGEYECKAQGTL
                     P"
     gene            complement(8967..9563)
                     /locus_tag="VFQ61_12705"
     CDS             complement(8967..9563)
                     /locus_tag="VFQ61_12705"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9955364.1"
                     /translation="MERFRRFDEAYYHRYYESPSTRVISPEEHANLAEFVFAFARYNR
                     IEIKSVLDIGAGVGLWKYWIEKNTKSVQYTGTEVSQAMCKKHGHQFRDIARWRDRKKH
                     DLIVCQGVLQYLPDPDVAPAVANIAAMARGLVYFEVTTRTDLRERCDKTKTDSDIHVR
                     NGSYYRGIFAKHLVSVGCGLWWVKDRPPPFFELEMAGG"
     gene            complement(9760..>10165)
                     /locus_tag="VFQ61_12710"
     CDS             complement(9760..>10165)
                     /locus_tag="VFQ61_12710"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HET9955365.1"
                     /translation="ERLLKRVPLVKLIYTSIKDLIGAFVGDHRSFDRPVAMRLAADSP
                     VRMLGFVTREALHTLGFVEHVAVYLPQSYNFAGNLVLVPRALVEPLDVSSGELMTFIV
                     SGGVSGLGIGPSMLPPAPLSKGSPRREPPGRA"
BASE COUNT         1827 a         3090 c         3323 g         1925 t
ORIGIN      
        1 ccggcagcgc tgagttggcc cgcgccaccg agattgggat cctgctcgag gacacgcagc
       61 gaatcgaagc ccagttcggg ccatacaagt cagacccgga cgtgctcgcg atcgtcgtga
      121 ccgacgcgca cggagcgccc ctcgttctgc atggctctct gccgcagccg ctcaccgaga
      181 tattttcggg ccccgagggt ggtttgagtc gcctgcccaa cgcctacgta tcgtggcggc
      241 gcgccatggt cgagggcatc gaggtggggc gggtcgcggt ggcagcctcg aacctgcgcc
      301 tcgacgaagg ctcacggctc gagcgacgca ttctgctcgg cgcgttcatt ggaggcttgc
      361 tggcatttgc ggcggcactg gggttcgtcg ccttttatgt acggcccgtg atccgcgtga
      421 cgcaggctgc atttgctcgc ctcgaaaaga cgaccgagct cgcactgcag gccgcccgca
      481 tgaagagcga gttcctcgcc aacatgagcc acgagattcg tacgccgatg aacggcgtgc
      541 tcggcatgat cgagctgctc aatggcacca cgctggaagc caagcagcgc cgctataccc
      601 agacgctaca ggcctccgcg gccgccctga tgacggtatt gaacgacatc ctggacttct
      661 ccaagatgga agccggcaag ctccggctgc aaccagacgc gaccgagctg cgaaccgtgg
      721 tcgaagaggt cgccgagttg ttcggggcgc gcgcccatct gcgtaacatc gaattggctt
      781 gtcacatcga tcgggagctg ccgcgcttcg tcgaggtgga ctccgatcgt ctacgtcaaa
      841 tcttgtcgaa cttgatcggc aacgccgtca aattcaccga tcacgggcag atcgtgattc
      901 gggtgaagtt gctcgggccc gctggcgatt cgaaatccaa gcggcgggtc gacgtgcgct
      961 tcgaggtgga ggatagcggc gttggcatcg atgcggcgac acggggacgc ttgttcgagg
     1021 ccttttccca agctgacgga tcgtttaccc gcgagcacgg gggaaccggc ttggggcttg
     1081 ccatctgcag caagctcgtc gagctgatgc gggggagtat aggtgtcgag agcgaggttg
     1141 gttgcggcag caccttttgg ttcgttctgc cgctggagcc ggtcgagatg gagcaggcca
     1201 gcgaacgcgg cctgcatctc cgccgagcgc gcactctgat cgtcgacgac aacgagacga
     1261 atcgactgat tctggaggag ctgctctcca gttggggctt tccgaaccag agcgtacccg
     1321 acgtggacag cgcgctgcgc gcgttgacgc gggcgcagcg tagcggcgaa gcgttcgggc
     1381 tcgtgatttc ggacatgcat atgcccgagg tggacgggct cacgcttgcg cgccacgtgc
     1441 gaggtagcga acacggagat ctgcctctga tcctgctcac ctcgcaaagc gaggaactac
     1501 caccggagga gctcgatcta ctcgatggga tcttgcaaaa gccggtgcgc gccgagcaac
     1561 tgtcgagcgt cattcgacgt gcgttgcaca gcggtaccgc ccgccacagt agccctggcg
     1621 aatcggcaga gtcgagaacg cagaagctcg cgcattccaa gattctggtc gtggaggata
     1681 atcccatcaa tcaggaagtg atttgcgaga tgctgcgaga actggggatc ggggcggtcg
     1741 tggccgacca cggcgcgcac gctctcgagc tcttggcgac gcacagcttt cccgtcgtgc
     1801 tcatggactg tcagatgcca gtcatggacg gctacacggc aactcgcgag atccgtcggc
     1861 gcgagaacgg cgcttcgcgt atgccgatca tcgccgtcac ggcccatgcc atgcagagcg
     1921 agcgcgacaa ggtacaagcc gcggggatga acgactacat ctccaaaccc atcacaagac
     1981 gagcgctcgc cgcggccctg caacgctggt ggccggagct ggcggagagt gcgccgatct
     2041 cgatcgtgaa cgtgccgcgg acggtcttgc cacagccctc gagtttggat cgcgcggtga
     2101 ggcgcagcgc ggtcgtgatt cggagcttcg tgcggcacgc accgagccag atcgaggcga
     2161 tccagagcgc gctgcgagaa ggggatgccg ctgcgctcaa gcaggctgcg catctcctga
     2221 agggcggttg cctagcggtg ggtgtgccac gcatggcgag tctttgcgca cgtctggaag
     2281 tgaatccggt cgatcgcctg gagctgtgta gtcaactcga acgagagtac gccaaggtgc
     2341 aggcgcgctt ggaagcgttg ctcgtcagtg agcctcagag cgtcgaggcc tctcgataca
     2401 agcgatgaga gttggacgtc ggcgctgcgc atcggcctga gacggcgcta aagcacgaat
     2461 tcgttttgcc gtttgcttct cgcgaggcaa acacgtgaag tacttaggcg aagttctccg
     2521 acgcgggcgc tcgaggaccc gcggaagcaa agcttccacg cgaatccgag cggcggtgct
     2581 cgtctccttg atgggaagtg cgctcgcagc tgcgtgcaca ttcgatccga gtgaccgctg
     2641 cggtccgaat caacaagttt ggggcgacaa tgtgcgttgt gtctgcagcc cgggatatgg
     2701 gtggactcag agcgacggct gcatgaagtg cccggatgat caacagacgg gcccactcgg
     2761 aacctgcgtt tgcaagaccg gatacgtgct cggagccgcg ggttgtgagc caattccggc
     2821 ggggctcggc gtggattgca gcgcctccgg tatgtgcggt gcggagcctt attcctattg
     2881 catggagacg gaccgcggat cctactgcac gagcaaaggc tgcaaaacgt cggccgattg
     2941 cgcgttcggc tatgcttgcg acgtgccgag cggtgtgtgc ctcaggcccc ccgtcggttt
     3001 gtacatgacc tgccagtccg acaaggactg tgcggacacc gaagccactt attgtgacgt
     3061 cttcttctcc cattcttgct tggtgcaggg atgcacggtc tcgccgaaca actgcttcga
     3121 gggctccgag tgctgcgacc tgagcacgtt cggagtcgca caaccactct gcattccggc
     3181 ggggagttgc accacatgag aacacggctg atatcgaatc tggtgttggg cgcgctagtc
     3241 gccgcgtact tgggtgcttc tccggcggcc acggcgcaaa ccgcagctgc gagcccgggt
     3301 gcctcgctac ccactagtcc ggtcgccccc gcgccggcgg ccgcggcagc tcccgaatcg
     3361 gaactcgagg gcccgattcc cgcggcggct acggccccgg cgctcgtcgc ggagacgcct
     3421 gcgagcgcgc ccgccgcgcc cgtagaagtt ggtgcggcgg cgaccagcgc cgccgacgct
     3481 gcggacgcgt ccgcctcgag tgtgccggcg gcccccgcaa gcacgccaac ggcggaagaa
     3541 cccctggatc tctcccagtt cacccagagt gacaccagct cgataggctc cgacgaaggc
     3601 aagctcaacc tctatggctt ttcggacttc tcgtatttcc ttccgctccg acgcggaatc
     3661 gggatcacgc cgtacccgac tttctacgta ggtaatctga atctgtatat ggcgaccgat
     3721 ctgggcgcgg acttccggtc catggtcgaa gttcggttct cctacttgcc tcacggggcc
     3781 acgccgttgg ctgccgacgg cttcaccgct ttgccgcgcg tcaacaatac cgcaccggac
     3841 tataccgact tgggtcgtcc gctacgctgg ggtggcatct ccattcagcg ggcttggctc
     3901 gagaagacgt tccacccgtt ggcgaccgta cgagtaggcc attggctcac cccgtacggt
     3961 atttggaacg tagatcacgg ttcaccggtg atcatcagcg tgcgccggcc ctatatcgta
     4021 ggtgaaagct tcttcccgca gagccagact ggcatcgagt tgtacggcag caccgccttc
     4081 ggccccgcgc aggtgggcta tcacctgacg ctctcgaatg ggcgcggacc gctggatact
     4141 tatcaagatc tcgatagcaa caaggccgtg ggcgtgcggc tatggggcaa gtacgattcg
     4201 gactttggta cgttcacgct cggtacgtcc atgtatcggg gccagtacgc ggatcgcacc
     4261 gcgacctacg tcatgaacga gtccggagcc ttcgacagca aatggctgcc caccgtcaag
     4321 tacgacgagc tcagccttgc ggctgacttc aaatgggagt ggaagggcgc gctgttccaa
     4381 gccgaaggga tcttgaacga cgtgacttac aagaccgaag agaaactgcg gccggtcgtc
     4441 acatcggcgt acggcggccc gaatggatac acccccgaca gtcgtcgctc gggcgcgtat
     4501 ggtctgattg gttaccgcac cgactttttg ggcacgatgc cgttcttcgg cggtgagttc
     4561 tatcacgttg gcatgcactc ctttacgcct gacgcggcgg cgatctggac ggggctcaac
     4621 gtgcggccca cgccacgcgt ggtcctgaag gccgtgtata gtcgctcctg gttcacgaac
     4681 acatcgccgt ttggtgcgag cttcgagtcc ttcgaagcgc tcgatttcca ggccgcatgg
     4741 ggtttttgag atgatgacaa ctcgacgcca gctgttcccc atgactctcg ggctgcttgc
     4801 cgggctcggg ttgaatcgca gcgtgtttgc caaggatgcg ccatcccgcc tcgcggtcgt
     4861 ggtggccaag agtagccctt tgcagagcat cggtttccac gaactgaagc gcctgtacat
     4921 gggtgagacc gtgaacggtc cggaaggaat gcgcctggtc gcgctgaatc atgcgccgct
     4981 ctcgacggag cgcgtacaat tcgacaagtc ggtgctcggg atgtcgcccg aagaggtcgc
     5041 caggttctgg atcgaccgta agattcgcgg gcaatccggc gcgccaaaga cagtggagcc
     5101 gctcgatcta ttgcaacggg tggtggctca gctgcgtggc gccgtaggct attgtcgcct
     5161 ggaggacgtg aagggtgagc tccgcgtcat caaggtggac gggaggctcc cgaacgaagg
     5221 cgggtatccg gtgtccacgt gagcaaccat caccataaac aggcagttct gccatgatcg
     5281 gccggcgtgc gttgttccca ctctcggcct tcgtcttggc tgagctcggc ttcgcgccgt
     5341 cttgctgggc tgcaggcgag ggcggatcgc gtctcgccat cgtagtcgca aaaggcagcc
     5401 cgctgcagag tatcggcttt cacgagctaa agcgcctgta catgggtgaa acggtgaatg
     5461 gccccgaagg cctgcgcttg ctcgcgctca atcaatccag tgtgtccgcc gagcgcatgc
     5521 gtttcgatcg gggggtgctc ggcatgaccc ccgagcaggc ggcgcgctac tggattgatc
     5581 gcaagatccg cggccaacct ggcgccccaa aagccgtgga catggtcgag gtattgcagc
     5641 gcgccgtggc ccaattgcgc ggggcggtcg gttactgtcg actggacgag gtgcgtgatg
     5701 ggctacgcgt gctgaagatc gagggcaagc tgcccaccga cgcgggctat cctgtttcgg
     5761 gctgagcgcg cccgagccac tgccggtagt ctccaaagac ggcgggcgtg cgcccgtgcc
     5821 gatgcttggg ctaaggtgct ggcgcatgac cgaagcgttg tctctaggtg tcgatctcgg
     5881 cggaaccaaa gtcgaggccg tgttgctgcg ccgaacgcca gccggacccg gcttcgaaat
     5941 cttgacgcgc gagcgagtcg caacaggaag cgaacgcggc tacgaagcga tcctcaccac
     6001 caccgcggag ttggtccgga atgtcgcgcg gagcagcggt cgtccgctgc tcgagttggc
     6061 cgtgggtgtg ggaatgccgg gttcggtctc gcgaacgacc gggctggtca agaactcgaa
     6121 caccgtgtgc ctcaatggga ggccttttag ggaagacctg agtcgtgcgc tcgggtgccc
     6181 ggtggccttc gagaacgacg cgaactgctt tgcgttggcg gaggcctact tgggtgcggg
     6241 ccgggcccat cgcaagggga ttgtgttcgg agtaatcctg ggcagcgggg tcggcggggg
     6301 aatcgtgatc gacggacgag tttggccagg gccgcaaaac ttggcgggtg agtggggcca
     6361 tcacgcggtg gggccgtggg ctcgagtgga gatgctggcc ggagccgctc cgagcgccgc
     6421 tggattcgca gcctgtggtc tatctccgcg ggcacgctgt tattgcggaa aaatgggctg
     6481 tttggagctt tacgccagcg gggtcaacgt cgaacgcgag tacgagcgtc gcacggggcg
     6541 gcgcaggatc ctgaaagagc tggtggcgtc tcggatcgac gacgtcgatg cggcgagcgt
     6601 cgtggacgaa atgatcgagg ccatcggacg cgggctcgcg aacgtcatcg atatcctgga
     6661 tccgagcgcg atcgtgctcg ggggcggggt atcgaacttg gacattctct acggggaggg
     6721 tgtggctcgc gtggctcgct acctgttcaa cgatgaactc cgcacgccta ttctcaagca
     6781 cgaactggga gactcagccg gcgtacttgg cgcggccttg ttgcatacgg acgagtacac
     6841 acacgccagt gaatgagcag gcccccctcg ccgaaaagaa cggcccggtg cgaacccacg
     6901 atcctcgcgc atacgctgcg atgccccccg ccgtcgcgtc ccggcccgac gggccacgca
     6961 gagcgcagcc gcagccgcag cggcagagcg cagcgtagcg gcagagcgca gcgtagcggc
     7021 tcagcgccgg tagcgcgaga ttcggggctg gctcactgcg gccaaggtat gagccaacgc
     7081 cgtccaggtc atcccgtcac ccccgagggt ttgcgtcgag ctcattacgt aagccaaatc
     7141 cccgagcgaa ataatcccga tcaggcgacg ttcggcgtcg accaccggca cgcgccgaat
     7201 cttgtgttgc tgcatcaagg cttgcaccgt ggcaggcgtg tcttgaggcg cgcagcactt
     7261 cacatcgcgg gccatcgcac tcgccacgct cgactcgcgc aacgcgacgc cttgaagctg
     7321 ggcggccatc gcgaggtcgc gatcggtgat gatgccaacc aactttccgt cgcgatccac
     7381 gaccggcacc gcgccacagt cgttttccca catgatctgg gctgcggtgg ccagcgtgtc
     7441 gtgcggcgta cagctgctca cgtcgcgcgt catgatttct gcgatcttca cgagcgtctc
     7501 ctatttcgcc caagtcttgc gtaatgcttc gagctcggcg gggcacagct taccgacaca
     7561 agctccgctg cgcgagcgcg acgcgcacgg aagcgtcaga aggtaggcgt cctgagcacc
     7621 tcggtgtgcg tgattcgccg gggcagcgcg ggactgggcg gcatcgcgct gttccgctcc
     7681 gtggtcgtcg gctggaaggg cgggctgatt ggtgggctga ttcgtaggct atagtggggc
     7741 gtggacttcg cacactatgc tcagcggccg gacgcccagc tcgcgctgct cgagggcagc
     7801 gttctaattg cgcaggacga gtatcctggt ttccagctcg ccaacgccga ggctgccatc
     7861 gaggagttgg cgctaccgct cgagtcgctg cagttgagcc gtatgtctgc gcgcgcccag
     7921 gcacgagcgc tatccgaaca tttgtatgta gcctgcgggt ttcgcgggga tgtggagacc
     7981 taccacgctc cggagaactc ctatctaaac cgggtgctcg agcgtcgtca ggggattccg
     8041 attagcctgg ccgtggttta catggaggtc gcgcggcggc tgtccgtaag cgctcggggc
     8101 gtcggttttc ccgggcactt cttggtgcgg ctcgatacgg aacacgactc cgcgatcgtc
     8161 gatccgttct ttggcggtga aattctcgac tccgcccgct tggagcagct gctgggcaac
     8221 atgcgtccgc gcctgaagtt gcaccaggac atgctggagc ccgcgagcgt acgggcaatt
     8281 atcgcgcgca tgctgacgaa cctgcgcgcc atctattccg cccgagccga cgccgggcgt
     8341 ctgctcgtcg tactggatca tctgagcgac ctgttgccgg acgcagtgga cgtggtccga
     8401 gatcgcgggt tcttgcacgc gcgaatgggc gccaacgcgg ctgcgatcgc ggatctccag
     8461 tgttacgtcg acgtgctgcc acatgcggac gacgccgagc aaatccgggc ggctatcgag
     8521 cggatgtcgg gcagcagcag cacacaaaat tgagacctct gcaggcgcga cagtggattc
     8581 gggacgaaag tcagggcccg aagtagggcc ctccgaactc ccgccgtgcg ccaattctcg
     8641 tcaaaacttt acggtagtgt gccttgggct ttgcactcgt attcgccgac caaccaaaag
     8701 gcgcgcctgc agtcgacgtc caccgtggcg cgcctccgat ccttcggtag cttcagctcg
     8761 acgcggaacg tgaaggattg tgagatgggt gagcgtgact cacgctgcag ctcgtgatcg
     8821 cagggccgct cggaacaggc caaccgcgcg gcttgcgcct tgagctcgct gttatcggag
     8881 tacacgttgt acagcccaac gccggttccc acgatcaaca gcaggctgac gaggttacgc
     8941 gcgaatccac gttccgccat gcgccctcag cctcccgcca tttcgagctc gaagaacggt
     9001 ggcgggcgat ctttgaccca ccaaaggccg cagcccacgc tcaccagatg cttggcgaag
     9061 atcccgcggt agtacgagcc gttcctgacg tggatatcgc tgtcggtctt ggtcttgtcg
     9121 cagcgctctc gcagatccgt gcgcgtggtg acctcgaagt agaccaaccc gcgcgccatg
     9181 gccgcaatgt tggcgaccgc gggcgcaacg tcgggatcgg gcaagtattg gagcacgccc
     9241 tgacacacga tgaggtcgtg cttcttgcgg tcgcgccaac gcgcgatgtc acggaactga
     9301 tgcccgtgct tcttgcacat ggcctgactg acctcggttc cggtgtactg gactgacttg
     9361 gtgttctttt cgatccaata cttccacagc ccaacccccg ccccaatatc gagaacgctc
     9421 ttgatttcta tgcggttgta gcgagcgaac gcgaacacga actcagctaa gtttgcgtgt
     9481 tcttctggcg agatgacccg agtcgatggg gattcgtagt agcggtgata gtacgcttca
     9541 tcgaagcgcc ggaaccgttc catgaggagg attctactcg aacgtgggtg ccttccgtcc
     9601 acgttgccgc gctgagtttc taaagggtga cgcctcctgc gcggcttcag tgcgggcctc
     9661 gggagtgatg atcggtgtcc ggttggccga ctcgaggcgt cgaagtcttg gctttttcgc
     9721 atgcccgaga cccaaagtgg cgcctccgcc accgagtttt tacgcgcgac ccggtggttc
     9781 ccggcgtgga gagcctttgc tcaggggcgc cggaggcagc atcgaaggtc cgatccccaa
     9841 gccagatacc ccgcccgaaa cgatgaaagt catcaattct ccgctcgaaa cgtcgagtgg
     9901 ctctactaag gcccggggca ccaacaccaa gttacccgcg aagttgtagg actgcggcag
     9961 gtaaacggcg acgtgctcga cgaaacccag cgtgtgcaac gcctcacggg tcacgaagcc
    10021 gagcatgcgt accgggctat ccgcggcaag ccgcatcgcg acgggccgat cgaagctgcg
    10081 atgatcgcct acgaacgcgc caatcaagtc tttgatcgac gtgtagatca gcttcaccag
    10141 aggcacccgc ttgagcagtc gctcc
//
LOCUS       DASTKU010000369         7247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1855345, whole genome shotgun sequence.
ACCESSION   DASTKU010000369 DASTKU010000000
VERSION     DASTKU010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855345"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFQ61_12715"
     CDS             complement(<1..732)
                     /locus_tag="VFQ61_12715"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET9955366.1"
                     /translation="MFALFGALGECTLTRLAQAVTTQAIASSTLALCITYVVHGLLWA
                     GVGALSAAHPKLPVPVRNRIWKAALLGPFVTVLLASTPLQALARAPLAKFALPNFTLT
                     LSAIPPGSALSGSSAGAGSAGDAVAGITGSLSLTAVGLATMLGLLRFGAAAHVARSML
                     SRRRPVRDARIAARFKAMCVRMGASNLLLTESTRLLCPMVVGRREICIPEGLLAGLPD
                     AQIDAVFGHELAHIERRDGIWFPIAG"
     gene            complement(735..1106)
                     /locus_tag="VFQ61_12720"
     CDS             complement(735..1106)
                     /locus_tag="VFQ61_12720"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HET9955367.1"
                     /translation="MGTEALTDLQLAVMKALWKVGEGGVAEIVTAMAEQGRVLAPTTV
                     ATLLQRLSKQGWVAHHKSGRQLLYRAQISEKEAAQGVLQRVVQAFFGGKVSAVAAQLL
                     ESDGLTAAELAELRRLIDEKR"
     gene            complement(1217..1747)
                     /locus_tag="VFQ61_12725"
     CDS             complement(1217..1747)
                     /locus_tag="VFQ61_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955368.1"
                     /translation="MKAPLLIGLVALAACSPREPPHWPEGGARLAMGTARWTRPGDEP
                     IELRRDGRVFEGGDLLFVIDRVGRIVDRDYEPLAILLPDGQLVDVDAQGLGQIGLANA
                     APPWTEAAWLSVTADGHVIHFDPDGDRESLGNFEGCAGAVHRTCTVVAHMVQLRQVRT
                     NPSGHVRVGVGVGIGF"
     gene            complement(1859..2605)
                     /locus_tag="VFQ61_12730"
     CDS             complement(1859..2605)
                     /locus_tag="VFQ61_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9955369.1"
                     /translation="MSDASALEVDQLQVHYGGIAAVKGIHLRVARGEIVTLIGANGAG
                     KTSTLKAIVGLVPSTGQIRLFGEEVRGKPCHRIVARGVALVPEGRAVFASMSVLENLE
                     LGSFLKRDSKARAQRLERCIQLFPRLGERLHQEAGTLSGGEQQMLAIARALMGDPKLI
                     LLDEPSLGLAPKLVQQIFASIVEIAKTGITVLLVEQNTRLALETADRGYVLLTGEVVL
                     SGEAKRLAEDPRVRAAYLGEEVASSVPPSG"
     gene            complement(2602..3411)
                     /locus_tag="VFQ61_12735"
     CDS             complement(2602..3411)
                     /locus_tag="VFQ61_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9955370.1"
                     /translation="MSLLSVRGVDKTFGGLHAVSSVSLELRERGIFGLIGPNGAGKTT
                     LFNLITGVYRPDAGQVRFAERDITGLQPAQIAALGIARTFQNIRLFRAMTVEQNVMVA
                     GHHVHRASVLSAMFRTRRHAEDESALRSRALELLELFNLRDCASDIAGSLPYGSQRRL
                     EIARALMLGPKLLLLDEPAAGMNSQEAEGLKRQVRFLRDELGISLLLVEHNMSVVMGV
                     CDVVHVLDQGRTIAEGEPLSVQNNPRVIAAYLGRDTEPPPASGAAAPENAP"
     gene            complement(3408..4424)
                     /locus_tag="VFQ61_12740"
     CDS             complement(3408..4424)
                     /locus_tag="VFQ61_12740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9955371.1"
                     /translation="MANSSRPAPARIALTRAAWVLGSMLGLYALHALLSAVLVEYAQR
                     IFLLAGINVILAVGLNLINGTTGQFSIGHAGFMAVGAYTAAFVGAQLRHVLPAGDPLI
                     DAVTFNLALLSGALSAGLAGLVVGVPSLRLRGDYLAVVTLGFAEIIRISFNNATFLGA
                     ATGYFGEDAAGLPPYTNFFWVFGWAALIVVLISNLTFSQTGRSLVAIREDEIAAEAMA
                     TPTTRLKVLAFALSAAAAGIAGGLFAHMQAGIRPEDFKFEKSIDMIVMIIVGGLGSIS
                     GAVIGGVFVAVTLELMRDLAQYRLVLYALLLVVIMIVRPQGLLGGREIRGFLRSGRKT
                     GAGR"
     gene            complement(4424..5341)
                     /locus_tag="VFQ61_12745"
     CDS             complement(4424..5341)
                     /locus_tag="VFQ61_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9955372.1"
                     /translation="MTELVQQIINGLSLGSIYALIALGYTMVYGILRLINFAHGEVFM
                     VGAYAGYYAASMLGVEAYEASGQPFPALFALLVLLLAMLAAALLGVSIEFFAYRPVRS
                     APRLTPLITAIGVSLFLQNAAMLVFGPNPRRYPAIVREVRYELFGVIVTNVKLLIFGV
                     ALALMLALGFLVKRTWMGRAMRAVSLNLDAAKLMGIDTHRTIRHCFAIGSALAAAGGI
                     LFGLDQIMITPLMGVLTGLKAFVAAVLGGIGNIPGAVIGGLLIGLAEQLTAGYLSPDY
                     RDAITFVILILILLLKPEGLLGVVRQEKV"
     gene            complement(5338..6522)
                     /locus_tag="VFQ61_12750"
     CDS             complement(5338..6522)
                     /locus_tag="VFQ61_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9955373.1"
                     /translation="MNCTRRDLLATLSLAALPLAGLGCKKPGGGSGTSGAASGPGKSP
                     EIVIGHYASMTGSTAHFGQDTDRAARLFVKQVNQAGGVLGKPIKLVTLDTRGDSAEAA
                     NAVSRLIDVEKVTAIIGEVASSLSLAGGRVAQRRKIPMVSPSSTNPKVTEVGDYIFRV
                     CFLDPFQGKVMATFARQNLKFEQVAILKDVKNDYSIGLADAFRAAFTALGGKIAVEQS
                     YSQGDTDFSAQITAIKGANVQAMFVPGYYSEVGAIARTAERLGVKVPLLGGDGWDAPD
                     LVKIAGDALNGSYYSNHFALDAADSRAQTFVKDFQAMYGVAPTGLGALGYDALAVVAD
                     AIKRANSVAPDKIRDALASTQGFDGVTGKINIDAQRNAQKSAVVLKIEAGKPKYETTI
                     SP"
     gene            6680..>7247
                     /locus_tag="VFQ61_12755"
     CDS             6680..>7247
                     /locus_tag="VFQ61_12755"
                     /inference="COORDINATES: protein motif:HMM:NF037053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955374.1"
                     /translation="MIVVMEPDAPRSSVERVIAFLVSLGFDVHQSSGQERTILGVVGD
                     LTGNDISVVSELEGVAHVVRVSEPFRLASRRFRQRPTVVEGAFGSIGGERPWIALEPV
                     GPEGTRGADESNPPASRELPYEVAAGRPFDAAVTRSRYALNNLGSLAWLSIHPHPAQQ
                     ASAVCFVERAPSWGAKHWIAAAERELARG"
BASE COUNT         1412 a         2397 c         2209 g         1229 t
ORIGIN      
        1 gcccgcgatg ggaaaccaaa tcccatcgcg gcgttcgatg tgggcgagct catgcccgaa
       61 aacagcgtcg atttgggcgt ccggcaaccc cgccaggagc ccctccggaa tgcagatctc
      121 gcgccttccg accaccatgg gacagagcag ccgggtgctc tccgtaagca agaggttcga
      181 cgcacccatt cgaacgcaca tggctttgaa gcgcgcggcg atcctcgcat cccgtaccgg
      241 acggcgtcga ctcagcatgc tgcgtgccac gtgcgcagca gctccgaagc ggagtaagcc
      301 caacatggtc gcgaggccca cggcggtaag tgaaagcgac ccggtgatcc ccgcaaccgc
      361 atctcccgca ctcccggctc cggcgctaga cccagatagc gcggatcctg gcgggatggc
      421 ggacaaggtc agtgtgaaat tgggtagcgc gaacttcgcc agcggagctc gcgcgagtgc
      481 ctgtaatggg gtggacgcga gcaagaccgt aacgaacggg cccagcagcg cagctttcca
      541 gattcggttt ctgaccggca cgggaagctt cggatgagcc gcactcagcg cccctactcc
      601 cgcccagagt aggccgtgca cgacgtaggt aatacagagc gccagcgtgg aactcgcgat
      661 cgcctgggtg gtgactgctt gcgccaagcg cgtcagcgta cactcaccga gagctccgaa
      721 gagagcaaac atgctcaccg cttctcgtcg attaatcgac gaagctcggc gagctccgct
      781 gcagtcagtc catctgactc gagcaattgg gcggccacgg ccgagacctt gccgccaaag
      841 aacgcttgca ccacgcgctg cagaacgccc tgggccgcct ctttctcgga gatttgggcc
      901 cgataaagta gctgacgccc tgacttgtgg tgtgcgaccc agccttgttt gctcaggcgt
      961 tgcaatagag tcgccaccgt agtcggcgcg agcactcggc cctgttcggc catggccgtc
     1021 acgatctcgg cgactccccc ttcacccact ttccaaaggg ctttcatcac agcgagttgc
     1081 aaatccgtga gcgcttcagt tcccactact acaggtgtag ttcctggcgg acgggtcgtc
     1141 aactacaaat gtagtttata gatgcgcctg gttcctcggc gacgaaggcg cggcgtaacc
     1201 gcgctaggac gcgatcttag aagccgatcc ccacgcccac gccaacacgt acatgcccac
     1261 tcgggttcgt acgcacctga cggagctgaa ccatgtgggc cacgaccgtg caggtgcggt
     1321 gaaccgctcc cgcacagcct tcgaagttcc ccaacgattc gcgatcccca tccggatcga
     1381 agtggatgac gtgtccgtcc gcggtaacgg acaaccatgc ggcttcggtc caaggtggcg
     1441 cggcgttagc gaggccgatc tgccccaggc cctgagcgtc gacatccacg agctgcccgt
     1501 cgggcagaag aatcgccagc ggttcgtaat cgcgatccac gattcgaccc acgcgatcga
     1561 tgacgaacag caggtctcca ccttcgaaga cgcgaccgtc gcgccgcaac tcgattggct
     1621 catccccagg ccgggtccag cgcgcggtgc ccatagcgag ccgggccccc ccttcgggcc
     1681 aatgcggagg ttcgcgcggt gaacaggcgg ccagagcgac caaaccgatc aagagcggcg
     1741 ctttcacgcc gcagagaata ccacgcccat cgccaagggc cgccgccctc ggccagcgtt
     1801 agcctcagtg cgaaccaaag ccgatgcgaa ccaaagccga tgcgaaccaa ggagagcgct
     1861 aacccgaagg tggcacgctg ctcgcgacct cttcacccaa gtacgcggcg cgcacgcgcg
     1921 gatcctccgc cagccgtttg gcctcaccgc tcaataccac ctcacccgtg agcagtacgt
     1981 agccgcggtc ggcagtctcg agagccaacc gcgtgttttg ctccaccagc agcacggtga
     2041 tgccggtctt ggcgatttct acgatgctag cgaagatttg ctgcacgagc ttcggagcaa
     2101 gaccgaggct cggctcatcg agcaggatca acttggggtc gcccatcaat gcccgcgcga
     2161 tcgcgagcat ctgctgttcg ccgcccgaca gcgtgcctgc ttcttgatgc aagcgctcac
     2221 cgagccgcgg gaagagttgg atgcaacgtt cgaggcgctg cgcgcgggct ttggagtcgc
     2281 gcttcagaaa actgccgagc tccagatttt cgagcacgct catgctcgca aagaccgccc
     2341 tcccttcggg caccagggct acgccccgag cgacgatgcg atgacaaggt ttgccccgga
     2401 cctcttcgcc gaagaggcgg atctggcccg tggatgggac gagcccgacg atggccttca
     2461 aagtgctggt cttgccggca ccgttggcgc caatcagcgt gacgatttcg ccgcgtgcga
     2521 cgcggagatg aatgcctttg acggcggcga tgccgccgta gtgcacctgc agctggtcga
     2581 cctcgagcgc ggatgcatca ctcatggcgc gttctccggc gcagccgcgc ccgacgcggg
     2641 tggtggctcg gtatcgcggc ccaaataagc ggcgatgacg cgcggattgt tttgcacact
     2701 gagtggttcg ccttcggcga tcgttcgacc ctgatcgagc acgtgaacca cgtcgcaaac
     2761 gcccatgacg accgacatat tgtgctcgac gagcaacagc gagatcccga gttcgtccct
     2821 caagaaccgc acctggcgct tcaagccctc cgcttcttgg ctgttcatgc ccgcggctgg
     2881 ctcgtccagc aacaatagct tggggccgag catcaaggcc ctcgcaatct ccagccggcg
     2941 ctgcgagccg tacgggagcg acccggcgat gtccgatgcg cagtcgcgta gattgaacaa
     3001 ttcgagcagc tccagcgcgc gcgagcgcag cgcgctttca tcctcggcgt ggcggcgcgt
     3061 ccgaaacatc gcgctcaata ccgatgcccg gtgcacgtgg tgaccggcca ccatcacgtt
     3121 ctgctccact gtcatggcgc ggaataggcg tatgttctgg aaggtcctcg cgatgcccaa
     3181 cgccgcaatc tgcgcaggct gcaggcccgt gatatcgcgc tcggcgaagc gaacttggcc
     3241 cgcgtccggt cgatagaccc ccgtgatcaa gttgaagagc gtcgtcttgc ccgcgccgtt
     3301 cggaccaatc aggccgaaga tcccacgctc ccgcagctcg agactgaccg agctcacggc
     3361 gtgcaaaccg ccgaacgttt tgtcgacgcc gcgcaccgac agcaagctca tcgaccggct
     3421 cccgtcttgc ggccgctgcg cagaaaaccc cgtatttcac gcccgcccag caaaccttga
     3481 gggcgcacga tcatgatcac caccagcagc agcgcgtaaa gcaccaggcg atactgcgcg
     3541 aggtcgcgca tcaactcgag cgtgaccgcc acgaacacgc cgccgatcac cgctcccgag
     3601 atcgagccca agccgcccac gatgatcatc acgatcatgt cgatcgactt ctcgaacttg
     3661 aaatcttcgg gccgaattcc cgcttgcatg tgcgcgaaca ggccgcccgc gatgccggcg
     3721 gccgccgcgc tcaaagcgaa cgccaacacc ttgaggcgcg tggttggcgt ggccattgct
     3781 tcggccgcga tctcgtcctc gcgaatggcg accagcgaac gaccagtttg ggagaaagtc
     3841 aggttggaaa tcagaacgac gatcagcgcc gcccacccga agacccaaaa gaaattggtg
     3901 tacggcggga gccccgcggc atcctcgccg aagtagcccg tcgccgcgcc gagaaacgtc
     3961 gcgttgttga aggagatgcg gatgatctcg gcgaagccca acgtcacgac tgccaggtag
     4021 tcaccgcgca aacgcaggct cggcacaccg acgaccaacc ccgccaaacc ggcgctcagg
     4081 gctccgctca acagcgcgag attgaaggtg accgcgtcga tgagcgggtc gcccgccggc
     4141 aacacgtggc gcagctgcgc acccacgaac gcggccgtat acgcgcccac cgccatgaat
     4201 ccggcgtggc cgatcgaaaa ttgaccggtg gtaccgttga tcaggttcaa gcccaccgcc
     4261 aagatcacgt tgatcccggc gagcaggaag atgcgttgcg cgtactccac gagtacggcc
     4321 gagagcagcg cgtgcaaggc gtacaagccg agcatcgagc cgagcaccca tgccgcgcgt
     4381 gtcagcgcga tccgtgccgg agcaggccgg ctggagttcg ccattacacc ttctcctgac
     4441 ggaccacgcc gagcaagccc tccggtttga gcagcaagat caggatcagg atcacgaacg
     4501 tgatcgcgtc gcgatagtcg ggcgagaggt aaccggcggt gagttgctcc gccaaaccaa
     4561 tcagcaaccc tccgattacc gccccgggga tgttaccgat gccgcccagc accgcggcca
     4621 cgaacgcctt caagcccgtc agcaccccca tcagcggggt gatcatgatc tgatccaaac
     4681 cgaagagaat gccgccagcg gcggcgagcg ccgagccaat cgcgaagcag tggcgaatcg
     4741 tgcggtgcgt atcgatgccc atcaacttcg cggcgtcgag gttgagcgag acggcgcgca
     4801 tggcccgccc catccaggta cgcttgacca gaaaaccgag ggccaacatc agcgcgagtg
     4861 ccacgccgaa gatcagcaac ttcacgttcg tgacgatcac tccgaacagc tcgtaacgca
     4921 cctcgcgtac gatcgccgga tagcgtcgcg gattgggacc gaatacgagc atcgcggcgt
     4981 tctgcaagaa caggctgaca ccgatggccg taatcaaagg cgtgagtcgc ggcgcggagc
     5041 gcaccgggcg gtacgcaaag aactcgatgc tcaccccgag caacgccgct gccagcatgg
     5101 ccagcaacag caccaatagg gcgaacaggg ccggaaaggg ctggccactc gcctcgtacg
     5161 cctccacgcc gagcatgctc gcggcgtagt agccggcgta cgcgcccacc atgaacactt
     5221 cgccgtgcgc gaagttgatc aggcgcagga tgccgtagac catcgtgtag ccgagcgcga
     5281 tcaaggcgta gatcgagccc agcgaaagcc cattgatgat ttgctgtacg agctcggtca
     5341 cggagaaatc gtggtctcgt acttgggttt gccagcctcg attttcagga ccaccgcgct
     5401 cttttgcgcg ttccgctgcg cgtcgatgtt gatctttccg gtcacgccat cgaagccctg
     5461 agtgctcgcg agcgcatctc taattttgtc cggcgccact gagttcgccc gcttgatggc
     5521 atcggccacc acggcgagcg cgtcgtaccc aagcgcaccg agtccagtgg gcgccacgcc
     5581 atacatcgct tggaagtctt tgacgaaggt ctgggctcga ctatccgcgg cgtcgagcgc
     5641 gaagtggttc gagtagtagg agccattgag cgcgtccccg gcaattttga cgaggtcggg
     5701 cgcatcccaa ccgtctccac cgagcagcgg caccttcacc cccaagcgct ccgccgtgcg
     5761 cgcgatggcg ccgacctcgg agtagtagcc gggcacgaac atggcctgca cgttggcgcc
     5821 tttgatggcc gtgatttgcg cgctgaaatc ggtgtcgcct tgcgagtagc tctgctcgac
     5881 ggcgatcttg ccaccgagcg cggtgaacgc agcccggaag gcatccgcca agccaatcga
     5941 atagtcgttc ttcacgtcct tgaggatggc gacctgctcg aatttcaggt tctggcgcgc
     6001 aaacgtcgcc atcactttgc cttggaaggg atccaagaaa cacacccgaa aaatatagtc
     6061 accgacctcc gtaacctttg gattggtgga tgacggagac accatcggga tcttgcggcg
     6121 ttgcgcgacg cgcccgcccg cgagcgacaa gctggatgcc acctcaccga tgatggccgt
     6181 gaccttctcg acgtcgatga ggcgcgagac ggcgttggcc gcctccgcgg agtcaccccg
     6241 cgtatccaac gtcaccaact tgatcggctt accgagcacc ccaccggcct gattcacctg
     6301 tttgacgaac aagcgtgctg cacggtcggt gtcttgcccg aagtgcgccg tgctccccgt
     6361 catcgaggca tagtggccaa tcacgatctc gggagatttg cccgggccac tggccgcgcc
     6421 cgacgttccg gatcccccgc cgggcttctt gcagcccaaa ccagccaggg gcagggctgc
     6481 cagggagagg gtcgccaaga ggtcccgacg tgtgcagttc atgtgttctc agctccgggc
     6541 accgcaggtt caaattgcaa gcttcggttc ttgcggccgg cccggcctcg gaaactaggg
     6601 agggaaagca ccgggggtca agtctcgcgc gcctcacgcc tgctttttgt gaagcggatt
     6661 tgctaccctt ctctctctca tgattgtcgt gatggagccc gatgccccgc gctcgtccgt
     6721 cgagcgagta attgctttct tggtttcgct cggcttcgac gtgcaccaat cgagcggcca
     6781 agagcgaact attttgggcg tagtcggaga cttgactggg aacgacatct ccgtcgtcag
     6841 cgagctcgag ggtgttgccc acgtcgtgcg tgtgagcgag ccgtttcgtc tggcgtcgcg
     6901 tcgtttccga cagcgaccaa ccgtggtgga gggcgcgttc gggagcatcg gcggcgagag
     6961 gccttggatt gcgctcgaac cagtcggtcc ggagggcacg cggggtgcgg acgaaagcaa
     7021 cccgcccgcc tctcgcgaac tgccctacga ggttgctgcc ggcaggccct tcgacgccgc
     7081 ggtcacacgc tctcgctacg cgctgaacaa cctgggatcg ctcgcctggt tgtcgatcca
     7141 tcctcatccg gcgcagcagg ccagcgccgt gtgcttcgtg gaacgggctc cgagctgggg
     7201 ggcgaagcat tggattgcgg ccgccgagcg tgagctcgcg cgcgggg
//
LOCUS       DASTKU010000370         6169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_70559,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000370 DASTKU010000000
VERSION     DASTKU010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70559"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1275)
                     /locus_tag="VFQ61_12760"
     CDS             complement(154..1275)
                     /locus_tag="VFQ61_12760"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HET9955375.1"
                     /translation="MRTLNSASPSNESASPQLSPNRALKRGASWLLLSSLVLSFGLAC
                     GGASSNTPAAASASSSGSKEPADDAPLTDPPDPPKAEGNPLVGLKMYVDPESLAQLRV
                     NALKTTDQGKAAIIEKIAKQPQALWLGEWNTNIFRTAQYNVEKAKDQGAVPIFVAYNI
                     PNRDCGQHSSGGTKTKDDYRRWIRKLAAGIGDGAAVVIMEPDALPMIDKENCLSQEQK
                     EERLFLLRDAVKVLRQNPKTIVYIDAGHPRWNPAEYMATQLKKAGIEFANGFSLNTSN
                     YVGTEDNLEYGKKLSGLLGGVHFVIDTSRNGNGPPSDPNEWCNPPGRKIGKPPTLETG
                     EPLADGFMWLKRPGESDGECHGGPRAGVFWDEKALELAQ"
     gene            complement(1530..2207)
                     /locus_tag="VFQ61_12765"
     CDS             complement(1530..2207)
                     /locus_tag="VFQ61_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955376.1"
                     /translation="MRAKTILFLVTTLVLSACGGGSKPAEEPSTDAGSAEKSAPKKSE
                     SESESDAEETSKTDAKSEKKDESESSTSGPKVSRTPKDMLTAPDIIFMFSFNGSDMKE
                     KAEQECDKKAKDDPKKRAQCMSKAKEKIEADGMRFLQEKGKWYWLVVRRKGKTLVNLH
                     KVPFEFGKETDHSIVLTPSGKDEGTQRTGSLGETTIEVPNEYQIVIQDPTHGKMVYEA
                     KIGITGG"
     gene            2580..3893
                     /gene="mgtE"
                     /locus_tag="VFQ61_12770"
     CDS             2580..3893
                     /gene="mgtE"
                     /locus_tag="VFQ61_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET9955377.1"
                     /translation="MQEERLTTSELRDAWAVLSPDERMDGFRQIDPEEADDFFSNLTE
                     REQAQMLLNMQPRERRLWFRQLPPDDAADVVQQIPKEDRGPFLELLDDASRREVTALL
                     AYAEDAAGGLMSPRFARLRPEMTVDEAIRYLRRQASERLETIYYAYVLDQEQRLCGVV
                     SFRDLFASPANTLLADVMHTSVISVQDTLDQESVARIMAEHDLNAIPVVDEQGRMKGI
                     VTIDDVVDVVQREATEDIQKLGGMEALETPYMQTSLAALVRKRAGWLAVLFLGELLTA
                     SAMSRYEDEISKAVVLALFVPLIISSGGNSGSQATTLIIRAMALGEITLRDWWRIVRR
                     ELAAGAVLGIILSVLGFARVVAWQAMFGTYGAHYIAVAATVGVSLVGVVTWGTVTGSM
                     LPFVIRRFGFDPASASAPFVATLVDVFGLVIYFNVGRFLLSGGLL"
     gene            complement(3895..5154)
                     /locus_tag="VFQ61_12775"
     CDS             complement(3895..5154)
                     /locus_tag="VFQ61_12775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9955378.1"
                     /translation="MSVTQELSSFRRASMLGRLMPLLTGLGLTAVTGWLAVPLPFALG
                     SGFAGGVVTAVLGFGFARLRQQDSEARDEAEVLSLIADSTPDAVLFFDDTGRIRYANP
                     SARDLFFEGASPVGKNFLSLVKEAPAALREALLGDSERLFSIEVEGQFETFHVSRRTF
                     EREQELHTLLVVKHLTREIARREVEVLKRVIRVISHEVNNSLAPVSSLVHSARKIAQG
                     PTPENKLERVFQTIEERTRHLASFLEGYANLARLPDPKPRRVEWAGFFRQIAELHPGV
                     SFASPPDQPGYFDAVQVEQVLINLLKNAVEAGSPRSGVEISARVDEAGDTELRVRDRG
                     PGFSEEALRSALLPLYTTKERGSGMGLALCREIVEAHGGSIRLSNAPDGGGWVSLRLP
                     GKPREPGRHLSRSRLTLTRSGQSQARN"
     gene            complement(5151..>6169)
                     /locus_tag="VFQ61_12780"
     CDS             complement(5151..>6169)
                     /locus_tag="VFQ61_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9955379.1"
                     /translation="ELGVRVADVAATLRLFVGGAEVSTYVEHGETYTVFVRGNSRYRA
                     DLDGLSLVTVPSGKFGSVPLLDVVRPEPGTGPASIDRANRRRQVMLLANITPGASQGE
                     IAAAVEREIQALHLPPEYRAVPFGQTREMARTGKAFMLAFALSFIFMYLVLAAQFESW
                     LHPITILLALPLTLPYALVSVLVFRQQLDIYSMLGLLVLFGVVKKNSILQIDHINQLR
                     REGKDRLTAILEGNRDRLRPILMTTFAFVAGMVPLLVSSGVGAGFNRATAGVIVGGQL
                     LSLLLTLLATPVAYSLFDDAAEFVKRFSTRGAKQRSVERERDLRELDGLDAMADGTLG
                     SPAE"
BASE COUNT         1072 a         1976 c         1933 g         1188 t
ORIGIN      
        1 gcccttatat acgttctggc cccccggacc cgaagctcgc acggcggtcc atgcgagctc
       61 cgagcggggc acgaccaatc cgggggtgac gacgagatct tcagccattc aagatgtttc
      121 ccgcgtcacc attgccggac cgcctcggcg cgcttactgc gcgagctcga gcgccttttc
      181 gtcccagaac acgcccgcgc gagggccacc gtggcactca ccgtcggact caccggggcg
      241 cttgagccac atgaaaccgt ccgcgagcgg ctcgcccgtc tccagcgtcg gaggcttgcc
      301 gatcttgcgc ccgggcgggt tgcaccactc gttgggatca gaggggggac cgttgccgtt
      361 gcggctggtg tcgatgacga agtgcacacc accgagcagc ccgctcagct tcttcccgta
      421 ttcgaggttg tcctcggttc ctacgtagtt cgaggtgttg agcgagaaac cattcgcgaa
      481 ctcgatgccc gccttcttca gctgcgtcgc catgtattcc gcggggttcc agcgtggatg
      541 acccgcgtcg atgtacacga tggtcttcgg gttttgacgc agcaccttca cggcgtctcg
      601 gagcaggaac aggcgctctt ccttctgctc ttggctgagg caattctctt tgtcgatcat
      661 gggcagtgcg tccggctcca tgatcacgac cgcggcgcca tcaccgatgc cggcggccag
      721 cttccggatc cagcgtcgat aatcgtcttt ggtcttcgta ccacccgaag agtgctgccc
      781 acagtcgcga ttcggaatgt tgtaagcgac gaagatcgga accgcgccct gatctttggc
      841 cttctcgacg ttgtattgcg ccgtgcggaa gatgttggtg ttccactcac ccaaccacag
      901 tgcctgcggc tgcttcgcga tcttctcgat aatcgcggct ttgccctggt ccgtcgtctt
      961 gagcgcattc acccgcagtt gcgcgagtga ctccggatcg acgtacatct tgaggccaac
     1021 gagcgggtta ccctcggcct tcggcggatc gggcggatcg gtgagcggag catcatcagc
     1081 gggctctttg ctgccactgc tcgaagcact cgcggcggcg ggggtgttgc tagaagcacc
     1141 cccgcaagcg agaccgaacg acagcacgag tgaagacaag agaagccacg aagccccacg
     1201 cttcagtgca cgattgggag atagctgggg cgacgcggat tcgttggaag gagaggcgga
     1261 gttcagcgta cgcatcgtgg ggccatatgt tgcattccgc agggaaaagg caaccccaca
     1321 catgccgtgt ctccgcctca tgactagcct catgttggcg ctcggccgag ggaccaagca
     1381 gccgcgacga ccgacggctg ccgcattccg caggacatgg ctccctgcgg catggcgcaa
     1441 acaccgacgc tcgtttgatg caaattgctt gatccgtagt gtggatttgg cacgcgcgcc
     1501 tgttcttcca ggtgagcttc gggtcgctct cagccgccgg taatcccgat cttcgcctcg
     1561 taaaccatct tcccgtgtgt ggggtcttga atgacgatct ggtattcgtt cggaacctcg
     1621 atcgtcgtct caccgagact accagtgcgc tgtgtacctt cgtccttgcc ggagggcgtg
     1681 agcacgatgc tgtggtccgt ttccttgccg aactcgaagg gaaccttgtg caggttgacc
     1741 aaggtcttgc ccttacggcg cacgaccaac cagtaccact tgcccttctc ctgcaggaac
     1801 cgcatgccgt ccgcctcgat cttctccttg gccttggaca tgcattgggc gcgcttcttc
     1861 ggatcgtcct tggccttctt gtcgcactcc tgctcggcct tttccttcat gtccgagccg
     1921 ttgaaggaga acatgaagat gatgtcgggc gccgtgagca tatccttcgg ggtgcgcgag
     1981 accttgggtc ccgacgtgga ggactcggat tcgtccttct tctcgctctt ggcgtcggtc
     2041 ttcgaggtct cctcggcgtc ggactcgctc tccgattcgg attttttggg cgcgctcttc
     2101 tcggcgctgc ccgcatcggt gctcggctct tccgcgggtt tgctccctcc gccacaggcg
     2161 gacaggacca gggtcgtgac taggaacagg atcgtttttg ctcgcatggc acctttccga
     2221 cggctgccgg cagagcgacc ttgggcgaaa ggccccgcgc catccggggc tgatgcttcg
     2281 agtcgaccct ctgtcggtag cccgctctgg tagcacgggg gaaagtcggg agaaaacacc
     2341 tggtcgcgcc aattgggcac gcgaacggca acgggcgcgg agccaagatt ggcgccggat
     2401 tgccgtcacc acccggcggg ggtgacgctt gccctcttcg agggccatcg ggccccggtg
     2461 agccagggcg cgttctaggg gcctcgatat ttttgcgggt tatgcaaact cccggcttta
     2521 cgcgatctac gatgaggagt atcgagccgg cgcgctttcg gcgggcgcgt gaggatccca
     2581 tgcaagaaga acgtctcacg acttcggagc tgcgcgacgc gtgggccgtg ctgtctccgg
     2641 acgagcggat ggatggcttc cgtcagatcg atcctgagga agccgacgac tttttctcca
     2701 acctgaccga gcgcgaacaa gcccagatgc tgctcaacat gcagcctcgg gaacgccggc
     2761 tctggtttcg gcagttgccg cccgatgacg cagccgacgt ggtgcagcaa atcccgaaag
     2821 aggaccgtgg gcccttcctg gagctgctcg acgacgccag ccgccgggaa gtgaccgctc
     2881 tgctcgccta tgccgaggac gcggcgggtg gtttgatgag ccctcggttc gcgcgcttgc
     2941 ggcccgaaat gacggtggac gaggcgatcc gctacttacg gcgccaagcc agcgagcggc
     3001 tcgagaccat ctattacgcg tacgtgctgg atcaggaaca gcgcctgtgc ggcgtggttt
     3061 cgttccgaga tttgttcgct tcgccggcca atacgctgct cgccgacgtg atgcacacca
     3121 gcgtgatcag cgtgcaggac acgttggacc aagagtccgt ggcgcggatc atggcggagc
     3181 acgacctgaa tgccatcccg gtcgtggacg aacaaggccg catgaaaggt atcgtcacga
     3241 tcgacgacgt ggtcgacgtg gtgcagcgcg aggccaccga ggacatccaa aaattgggcg
     3301 gtatggaggc cctcgaaacc ccttacatgc aaacttcgct cgccgcgctc gtgcgcaaac
     3361 gggcggggtg gctcgccgtg ttgttccttg gggagttgct cacggccagc gcgatgagcc
     3421 gctatgaaga cgagatctcg aaggcggtgg tgctggcgtt gttcgtgccg ttgattatct
     3481 cgagcggtgg taactcgggc tctcaagcca cgaccctgat cattcgcgcc atggcgctcg
     3541 gcgaaatcac gctccgggac tggtggcgaa tcgtgcggcg cgaactggcg gcgggggccg
     3601 tgctcgggat tattctctcg gtgttgggtt tcgcgcgtgt ggtcgcctgg caggccatgt
     3661 tcggaaccta cggcgctcac tacatcgcgg tggcggcaac ggtgggggtg agcctggtcg
     3721 gcgtggtcac ctggggcacg gtgactggct cgatgttgcc cttcgtgatt cgccgcttcg
     3781 gtttcgatcc tgcgagcgcc tccgccccat tcgtcgcgac gttggtggac gtgtttgggc
     3841 tcgtcatcta cttcaacgtc ggacgcttcc tactgagcgg gggtttgctc taggctagtt
     3901 gcgggcctgg ctttgaccgg atcgagtcaa ggtcaaacgc gagcgcgaga ggtggcgtcc
     3961 cggctcccgc ggtttacccg gtaggcgcag tgacacccac cctcccccgt cgggcgcgtt
     4021 gctcaagcga atgctgcccc catgggcttc cacgatttcc cgacataacg cgagccccat
     4081 cccgctgccg cgttccttgg tcgtatagag cggcaagagc gcgctacgca gagcttcctc
     4141 gctgaaaccc ggccctcgat cgcgtacccg gagctcggtg tcacccgctt cgtccacgcg
     4201 ggccgagatc tccacgccgg agcgcggact gccggcttcg accgcgttct tgagcagatt
     4261 gatcaagacc tgctccacct gcacggcgtc gaagtacccg ggctgatcgg gcgggctcgc
     4321 aaagctgacc cctgggtgca gctcggctat ttgccggaag aaccccgccc attccacgcg
     4381 ccgcggcttg gggtcgggca gacgggccaa gttggcatag ccttcgagga agctcgccag
     4441 gtgccgcgtc cgctcctcga tggtctggaa gacgcgttcc agcttgttct ccggagtggg
     4501 cccctgcgcg attttgcgag ctgaatgaac gagcgagctg accggcgcga gcgagttatt
     4561 gacctcgtga ctgatgactc gaatgacgcg cttcagcacc tccacctcgc gacgcgcgat
     4621 ctcgcgcgtc aggtgtttta cgaccagcaa cgtgtgcagc tcctgctcgc gctcgaaggt
     4681 gcgtcgactc acgtgaaagg tctcgaattg tccttccacc tcgatgctga acaagcgctc
     4741 ggagtcaccg agcagcgctt cgcggagggc ggcgggtgcc tccttgacca agctcaagaa
     4801 attcttcccg accggtgacg cgccctcgaa gaacaaatcg cgggcgctcg ggttggcgta
     4861 gcgaattcgc cccgtatcat cgaagaagag caccgcgtcc ggcgtcgagt ctgcgatcaa
     4921 tgacaggact tcggcttcgt ctcgggcttc gctatcttgc tgccgaaggc gagcgaaccc
     4981 gaagcccagc acagccgtca ccacgccgcc cgcaaacccg ctgccgagtg cgaacggcag
     5041 cgggaccgcc aaccagcccg tcaccgccgt gaggcccaaa ccggtcagca gcggcatcag
     5101 acgacccaac atgctggctc gacggaatga cgagagctcc tgggtgacgc tcattcggcg
     5161 ggagagccta gcgtgccatc cgccatcgcg tcgagaccgt ccagctcacg taggtcgcgc
     5221 tcgcgttcca ccgaccgttg cttggcccct cgcgtactga accgtttcac gaactcggcc
     5281 gcgtcgtcga acagcgagta agcgaccggc gtcgccagca gagtcaagag caacgagagc
     5341 agctgaccgc cgacgatcac ccccgcggtc gcccggttga agccggcgcc caccccgctc
     5401 gagaccagga gcggcaccat gccggccacg aacgcgaagg tcgtcatcaa gatcgggcgc
     5461 aaacgatcgc ggttaccttc caggattgcc gtcagacggt ctttgccctc acgccgcagc
     5521 tggttgatgt gatcgatctg cagaatcgag ttcttcttca cgaccccgaa caacaccagc
     5581 aagcccagca tcgagtaaat gtccagctgt tgccggaaca ccaacaccga gaccagcgcg
     5641 tacggcaggg tcagcggcaa tgccagcaag atggtgatcg gatgcagcca ggactcgaac
     5701 tgcgccgcca gcaccaagta catgaagatg aaagacagcg cgaaggccag catgaaggcc
     5761 ttgccggtgc gggccatctc gcgagtttga ccgaacggga ccgcgcgata ctccggcggc
     5821 aagtggagcg cctgaatctc gcgctccacg gcagccgcga tttcaccctg agaggccccg
     5881 ggcgtgatgt tcgcgagcag catcacttgt cggcgtcggt ttgcgcgatc gatcgacgcg
     5941 ggaccggttc ccggctctgg ccgcaccaca tcgagcagcg gcacgctccc gaacttgccc
     6001 gacggcaccg tcacgagaga gagcccgtcc aagtcggcgc gatagcggga attgccgcgc
     6061 acgaacaccg tgtaagtctc gccgtgctcg acgtaagtcg agacctccgc gccgcctacg
     6121 aacagccgca acgttgcggc cacgtccgcc acccgcacgc cgagctcgg
//
LOCUS       DASTKU010000371         4758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_962197, whole genome shotgun sequence.
ACCESSION   DASTKU010000371 DASTKU010000000
VERSION     DASTKU010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962197"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="VFQ61_12785"
     CDS             complement(<1..1651)
                     /locus_tag="VFQ61_12785"
                     /inference="COORDINATES: protein motif:HMM:NF015390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HET9955380.1"
                     /translation="MNRLSLLGISAFLIALPFTACDRGNPVRVPCANAVACAQGQECS
                     GGYCVAIQACSTAEPCPAAQKCVNNVCRNSCVDDAPCTPLGLRCDTAVGSCLPTSVPL
                     GTGGMAGATSGGSGAGGSASTMPGGGTAGTTSVPGGTGGSVVTDGGMTAVGGAPTGGA
                     GGAGGASGEQGPSYSLIDDLEDGNGRILEIDGRTGPWYVVSDGSGSPSATGTLTPEAD
                     AENKAVHFKLGGDASSWGSGVQVDLKNPGDDAKADSRSGWDASAYTGLTFRAKGNGSL
                     RLKLNTSSVVSQEEKGTCASDCFNAHGANLTLGSSWKTYVVVFGSETSGLPEGEKLTL
                     AQEDGWGTKVEFKASELMGLTLQGSKAFELWMDDVKLFKKGSTNPSNGTGGGGGMGTG
                     GSSGGGGECKDEFWSGYAAGQGSVTRYDFSQGTSLPACAYVITHKGAGRDGDWDTVEG
                     ISTGDGGYFGAMNTSDYKNGAACGACVEVSYNGKKVVATIVDECPIGTNPVCTAGHID
                     LSRKAIRQLESNASLEHLGKGGNGSVSWRYVACPVTGNVEMR"
     gene            1653..1805
                     /locus_tag="VFQ61_12790"
     CDS             1653..1805
                     /locus_tag="VFQ61_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955381.1"
                     /translation="MSTMANGRGGKRKDSGNPCRITLAPTFTQEIALDDRGWVREKFH
                     PERSGA"
     gene            complement(1854..4520)
                     /locus_tag="VFQ61_12795"
     CDS             complement(1854..4520)
                     /locus_tag="VFQ61_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9955382.1"
                     /translation="MSSEELSRFARGRVGSTLKGKWLLERLLGVGAMTSVYAAVHHSR
                     RRAAIKILNPDFSAEPTIRERFLREAYTANSVRHPSVVLVEDDDVDEEGCGFLVLEYL
                     EGESLETRRRRLGVLAPEETLSLIDPVLDALAVAHARGIVHRDLRPEHLFVARDGLTR
                     VLDFGMARIRDIGSHSYWTRVDPRFRETAYLAPEQRNGAYSQVNSRSDLWSVGAVMFT
                     LLTGVYPQRGRASLDGFQDIPPAVMRIIERALAERNDERWPDARTMLLEVRRAYFRLE
                     AHSEPTLNGEATYSRSLSSMPVSQPFTIRQHEPFALTPSVMALEAARRDQDDSGPDEE
                     AQTSAEPPPSRRITLADAAEDEESTGRNWPPSEPPPASLADADADDERTGRDWPPLSQ
                     PPPILISPSARAHEVRNAVPPPGIEVNKAVKTLPSPSREATPRESTSREATSRESSSR
                     KARVETARESTSREARESIESTPSATDVPRPAPRRSGFSPLHTAAVVAFGALIGVGLG
                     LLPSRSTQKDLNGRGLSRRVTTRALVDGRSNAADNERLAASSSASLSSVPASTSALTG
                     SPSSGATESSGPPAPAQRAQASQSNPGANGTTRAEAIREELKPAHAVFGERTALESTG
                     SGVGSESSAVGAESSLSQGSVPNLEHREQPLAARLPAPEAAFTATPRVDPSGAAMPVS
                     IPDHARVPAPVPSVSVPSAPAPAPIAKADLAGTALANPPAANPQPTLSAGASETNAPL
                     PPSATAATPAASSGIAPKLETSSPQAANTPRVVNTSQAVSTAPTVSTAQAVSTAQAVS
                     TAPFPREKAVARLRAARKQADDCRFPSGPNGMGTLRVSFQPAGGAPKVVLSPPFAGTQ
                     TGRCVSAVFQAVRVETFQGEPVTLVQSFEIPK"
BASE COUNT          825 a         1550 c         1493 g          890 t
ORIGIN      
        1 ggcgcatctc gacgttgcct gttacggggc acgctacgta gcgccaggag acggagccgt
       61 tgccgccctt gccgagatgc tcgaggctcg cattggactc gagctgtcga atcgctttgc
      121 ggctcagatc gatgtgcccg gctgtgcaga cgggattggt gccaatggga cactcgtcga
      181 cgatcgtggc gacgaccttc ttgccgttgt aggagacctc gacgcaggcg ccgcaagccg
      241 caccattctt gtagtcgctg gtgttcatgg cgccgaagta cccgccatcg ccggtgctga
      301 tgccttcgac cgtgtcccaa tcgccgtctc ggccggcgcc cttgtgcgtg atcacgtacg
      361 cgcaggcagg cagactcgtt ccctggctga agtcgtagcg cgtgacggag ccctgacccg
      421 cggcgtatcc ggaccagaac tcgtccttgc actcgccgcc gcccccgctg ctcccgcccg
      481 tgcccatccc gccgcctccg cccgtgccat tggaggggtt cgtcgagccc ttcttgaaga
      541 gcttcacgtc atccatccaa agctcgaaag ctttggagcc ttggagtgtg aggcccatca
      601 gctcggaggc cttgaattcg accttggttc cccagccatc ttcctgagct agcgtcagct
      661 tctcgccctc gggcaagcca ctcgtctcgc tgccgaacac gacaacgtag gttttccagc
      721 tagaacccag ggtgagattg gcgccgtggg cattgaagca gtcgctcgcg cacgtaccct
      781 tctcctcttg gctcacgacc gacgaggtgt tgagcttgag tcggaggctc ccgttgcctt
      841 tggcgcgaaa cgtgaggcca gtataagcac tcgcgtccca accgctgcgg ctgtcagcct
      901 tggcatcgtc acccgggttt ttgagatcca cctggactcc cgaaccccag ctgctcgcat
      961 ctccgccgag cttgaaatga acggctttgt tctccgcgtc cgcttcgggc gtgagcgtac
     1021 cggtcgcgct ggggctgccc gagccatccg aaacgacgta ccagggacct gttcgtccat
     1081 cgatttcgag gatgcgcccg ttcccatcct cgaggtcgtc gatcaaggag tacgacgggc
     1141 cttgctctcc ggatgcgccg cctgcgccgc cagcgccacc cgttggcgcg cctccgacgg
     1201 cggtcattcc gccgtcggtc acgacgctgc caccagtgcc gccaggaaca gaggtggtac
     1261 ccgcagtgcc accgcccggc atcgtcgagg ccgagccgcc cgctccgctg cctcccgagg
     1321 tagctcccgc catgccgcct gttccaaggg gcacgctagt cggcaaacag ctgcccaccg
     1381 ccgtatcaca gcgcagaccg aggggagtgc agggggcgtc atcgacgcag ctattgcggc
     1441 agacgttgtt gacacacttc tgggcagcgg ggcacggctc ggccgtgctg caagcctgaa
     1501 tcgccacgca atagccaccg ctacattcct gcccttgagc gcacgcgacg gcattggcgc
     1561 atggcacccg aacggggttt cctcggtcgc aggccgtaaa cggcagggct ataagaaatg
     1621 cactaatacc gagcaggctt aagcgattca tcttgagcac catggcgaat ggccggggag
     1681 gcaagcgcaa ggattccggc aacccttgcc gtatcacgct ggcgccaacg ttcacgcagg
     1741 aaattgcgct cgatgatcgc ggctgggttc gtgaaaaatt ccacccggaa aggtcgggcg
     1801 catgagaagc tcgccgcgtc aagctgtcta aaagagtttc gcggcctccc gactcacttg
     1861 ggaatttcga agctctgcac gagagtcacc ggctctccct gaaatgtctc tacccgtacc
     1921 gcctggaaga ccgcggagac gcaacgtccc gtctgagttc cggcaaacgg cggggatagc
     1981 accaccttgg gcgcgcctcc ggcgggttgg aacgagacgc gcaaggtacc catgccgttg
     2041 ggtccacttg gaaaacggca atcatcggct tgcttgcggg ccgcgcgcaa acgagcgact
     2101 gccttctctc gcgggaatgg tgccgtgctt accgcttgcg ccgtgcttac cgcttgcgcc
     2161 gtgcttaccg ttggcgccgt gcttaccgct tgggaggtgt ttactacgcg tggtgtgttt
     2221 gccgcttgcg ggctgctcgt ttcgagcttg ggggcgatgc cggaacttgc ggcgggtgtg
     2281 gcggccgttg cgctcggcgg caatggagca ttcgtttcgc tcgccccagc acttagggtc
     2341 ggttggggat tcgcagctgg aggattagcc agtgcagttc ctgcgagatc ggctttggct
     2401 atgggggcgg gagcaggcgc actcggaaca gacacgctgg gtacaggtgc agggacgcgg
     2461 gcgtgatccg ggatcgaaac cggcattgca gccccgctag gatcaactct gggcgttgcg
     2521 gtaaaggcag cttccggagc cggaaggcgc gccgcaagcg gttgctcccg atgctccagg
     2581 ttgggaacgg agccctgact taacgaagac tcggcgccaa ccgcgctgct ctccgagcca
     2641 acgccggagc cggttgactc gagagccgtg cgctcgccga acaccgcgtg cgccggcttg
     2701 agctcttcac ggattgcctc agccctcgtc gtcccattgg cgccgggatt cgactgactt
     2761 gcttgcgctc gttgggctgg cgccggcggg cctgaagact cggttgctcc ggaactcggc
     2821 gagccggtga gcgccgacgt cgaagcgggc actgagctga gcgaagcact cgaagacgcg
     2881 gctagccgct cgttatccgc ggcgttgctt cggccatcca ccagcgcccg cgtcgtcact
     2941 cggcgcgaaa ggccgcgtcc gttgagatcc ttttgggtcg agcggctcgg gagcagtccc
     3001 agccctaccc cgatcagagc accgaacgcg accaccgcag ccgtatgcaa cggcgagaag
     3061 ccactccggc ggggagccgg tcgcggaaca tccgtggcgc tcggcgtgga ttctatcgat
     3121 tcgcgcgcct cgcgcgaggt tgattcgcga gccgtttcca cgcgagcctt gcgcgagctt
     3181 gattcgcgcg acgtcgcttc ccgagaagtt gactctcgcg gcgtcgcctc acgcgaaggc
     3241 gaaggcagcg tcttcaccgc cttgtttacc tcgatcccag gcggtggcac cgcgtttcga
     3301 acctcgtgag ctcgcgcgga gggcgagatc agtatgggcg gtggctgtga cagcgggggc
     3361 caatcacgac cggtgcgttc atcgtcggcg tcggcatccg caagcgaagc ggggggcggt
     3421 tcggaaggtg gccagttgcg gccagtgctc tcctcgtctt ctgcagcatc cgcgagcgtg
     3481 attctcctgg aaggcggtgg ctccgcgctg gtctgggctt cttcgtctgg acccgaatca
     3541 tcctggtccc gacgtgccgc ctcgagtgcc atcacgctcg gcgttagggc aaatggctcg
     3601 tgttggcgta tcgtgaaggg ctgcgagacc ggcatgctcg agagcgatcg cgaataggtg
     3661 gcctccccgt tcaacgttgg ttccgagtgc gcttccaagc ggaagtacgc gcgccttacc
     3721 tccaacaaca tcgtgcgcgc atcgggccag cgctcatcgt tgcgttctgc cagagctcgt
     3781 tcgatgatgc gcatcaccgc gggcgggatg tcttgaaaac cgtcgaggct cgcccgaccg
     3841 cgctgcggat acacgccggt caacaacgtg aacattacgg cgccgaccga ccataggtca
     3901 ctgcgcgagt tgacctgact gtaagcaccg tttcgttgct cgggcgcaag ataggccgtc
     3961 tctcgaaacc gaggatcgac gcgagtccaa tacgaatggg acccaatgtc gcggatgcga
     4021 gccatcccga aatccagcac cctcgtcagc ccatcgcgcg caacgaacaa gtgctctggc
     4081 cgcaaatccc ggtgcactat cccgcgggcg tgagccaccg ccaaagcatc cagcaccgga
     4141 tcgatgagcg atagcgtctc ctccggtgcg agtacgccga gccgccgacg ccgggtctcg
     4201 agtgattcgc cttctaggta ttccaacact aggaagccgc agccctcttc gtccacgtcg
     4261 tcatcttcga cgagcaccac actcgggtgg cgcaccgagt tcgcagtgta tgcttcacgc
     4321 aaaaagcgct cacggatcgt cggttcagcc gaaaaatcag gattcaagat cttgatcgcc
     4381 gcgcgccgcc gactgtgatg aaccgctgcg tagaccgagg tcatggcgcc cactccgagc
     4441 aagcgctcga gcagccattt gcccttcaag gtcgaaccga cccgaccacg ggcgaaccga
     4501 gacaactcct ccgaagacac tcgttactcc tgcggtagct ggtgtcgggt taggcgttgc
     4561 ggatcgaagc cgcccaacac cacgcatcag gacagaattt cacgtccgca gtatagccaa
     4621 accaaatcga aggggcacct gatccgcgct tccgaggtgg cgcgccccgg cccgctggcg
     4681 tcaaccacgg tgtgtaacgg atggtgacct agtgaacgtg ttgacgagcg gccgagacct
     4741 tcggcaccgt tccgccat
//
LOCUS       DASTKU010000372         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_516935, whole genome shotgun sequence.
ACCESSION   DASTKU010000372 DASTKU010000000
VERSION     DASTKU010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516935"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..>2729)
                     /locus_tag="VFQ61_12800"
     CDS             complement(19..>2729)
                     /locus_tag="VFQ61_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955383.1"
                     /translation="SDDEADPGLPWVTVRKAWFGAGGDLGEHAALTHEAARRAGLPSR
                     LGEPFWVQYSVPNSYIASKLLNGQGWIAGQRWMPQKFGAPGTRALRGITLYELAQIPD
                     LSLSIGDWAAGNEVCFLDDIEGAFGPPPGQPGFDPGNLPCHSYVEAIGTINATHFMPA
                     ARNVYEHYHRIALAEMARCNTLASALSVPYQSWDWETVESASDTELHECEREALAFEM
                     FAQHFLQDAWSTGHMWYRWGHPTLSGFPFLLDEDGGSWDRPARNIPSENLQGRRGMIG
                     GAVAALSGMVHGSKAIATAVVRENAPWLSKISPNVFGLIDDPLSAPFYEDPMPSPFDV
                     DTLKPVMWRGANGLLARGAGDLFANLVLQDTQPALNPWRDRLLMCTATSLREVYEAGP
                     KAHGPLGDFTGAFERSNFQFDRECWSQRVTNASMFGSIAPARLTYSVPFQQNDPVMST
                     LFTLVNGMLVDRLELSRRLPNQPDAQEFLASLKKRMVRDQIRAATDYALNMGIMNDNG
                     AESSQGVRALPIAGDDQIRMLGVTPNRPLTADPPAYYMDVATPALPEALTPNDYFLRH
                     MFWRSHIDELCAEPNLAAQLRERCISAAVSPGGDPDACTQCVQVAELHMPSCVDNFGP
                     SKCGALGISETGGVPQEYLDFTAQLDLGLCAMPAYYAAFHYCTGTEPRLLSQGMLEGD
                     HVLEQTVTQTVPCSTYHPFLPYPDAELHRTRYRIGVGVAEGSAQIGYNYLAPLVSAFE
                     EETTNTLLDESALCGGASTFPQLLSVRTTSTLDGAVANAIAPAPLWDSNSNGMKALDS
                     ATVRDYEDKLKVEFCGETQRLSFYNKTCEVALNVIQQGSLAALQGMPYRASDGAQVVA
                     SSSGDQPRCSVREPRVFKPSCSRGTCNASGLCTAQAAPSVVRYYAR"
BASE COUNT          529 a          839 c          841 g          520 t
ORIGIN      
        1 agagcgacgt aatagagtct accgcgcata gtacctcacc accgagggtg ccgcttgcgc
       61 ggtgcagagc ccgctcgcat tgcaagttcc gcgcgagcag ctcggtttga ataccctcgg
      121 ttcgcggacg ctacagcgtg gctggtcgcc actcgagctc gcgactacct gcgcaccgtc
      181 ggacgctcgg tatggcatgc cctgcaacgc cgccaagcta ccttgctgga tgacgttgag
      241 cgcgacttcg caggttttat tgtaaaagct caaccgttgc gtttccccgc aaaactccac
      301 cttcagcttg tcttcgtagt cgcgcacggt ggcgctatcc agggccttca tgccgttgga
      361 gtttgagtcc caaagcggtg cgggagctat cgcgttcgcg accgcgccat cgagcgtgct
      421 cgtcgttcgc acgctcaaga gctgtgggaa ggtcgaagcg ccaccgcata aggcggactc
      481 gtcgagcaac gtattcgtcg tctcctcctc gaatgcggag accagcggcg caagatagtt
      541 gtatcctatt tgcgctgagc cctcagccac gccgactcca atgcggtacc tggtccggtg
      601 aagctccgcg tcagggtacg gaagaaacgg gtgatacgta gagcatggca ccgtctgagt
      661 caccgtctgt tcgagaacat ggtctccctc cagcattcct tgtgatagaa ggcgcggctc
      721 ggttcccgta caatagtgaa acgcggcgta ataggctggc attgcgcaga gcccgaggtc
      781 gagctgggcg gtgaagtcca agtattcctg ggggactccg cctgtttcgg agatgccgag
      841 agccccgcac ttggacggtc caaagttgtc gacacacgag ggcatgtgca gctcggctac
      901 ttgcacacac tgggtgcagg cgtcgggatc tccgcccgga ctcaccgcgg cagaaatgca
      961 gcgctcgcga agttgcgccg ccaggttggg ctcggcgcag agctcgtcga tatggctgcg
     1021 ccagaacata tggcgcagga aatagtcatt cggcgtgagc gcctccggca acgccggggt
     1081 tgccacatcc atataatacg caggtggatc ggccgtgagc ggccgattcg gggtgacgcc
     1141 cagcatgcgg atttggtcgt cgcctgcaat tggcagcgcg cgaacgcctt ggctcgactc
     1201 agcgccgttg tcgttcataa tccccatatt gagagcgtag tcggtcgcgg cgcggatttg
     1261 gtcgcgaacc attcgcttct ttaggctcgc aaggaactct tgtgcgtcag gttgattcgg
     1321 cagtcgccgg ctgagttcga gccggtcgac gagcatccca ttgacgagcg tgaacaacgt
     1381 cgacatgacg ggatcgttct gttggaaggg caccgaatac gtgagccgcg cgggagcgat
     1441 tgagccaaac atcgaggcat tagtgacacg ttgcgaccag cactcccgat cgaactggaa
     1501 attcgaacgc tcgaacgcac cggtgaaatc ccccagcggg ccgtgagcct ttgggcccgc
     1561 ctcgtacacc tcacgcaagg acgtcgccgt gcacatgagc agtcgatccc gccatgggtt
     1621 tagcgcgggt tgcgtatctt gtaagacgag gttcgcgaac agatcacccg cgccgcgcgc
     1681 cagcaagccg tttgcaccac gccacatcac cggtttcaag gtatccacgt cgaaaggcga
     1741 aggcatcggg tcttcgtaaa acggcgcact gagcggatcg tcgatcaacc cgaatacgtt
     1801 gggcgagatc ttgctgagcc acggcgcgtt ctcgcgaacg accgctgtgg caatcgcttt
     1861 ggagccgtgc accatgcccg acagcgccgc tacggcgccg ccgatcattc cacggcggcc
     1921 ttggagattc tctgagggaa tgtttcgcgc cggcctgtcc cacgaacccc cgtcttcatc
     1981 gagcaagaag ggaaagccgc tgagtgtggg gtgaccccag cggtaccaca tgtggccggt
     2041 cgaccacgcg tcctgcaaaa aatgctgcgc gaacatctcg aacgcgagcg cctcgcgttc
     2101 gcattcgtgc aactctgtgt ccgacgccga ctcgacggtc tcccagtccc aggactgata
     2161 agggacgctg agcgccgagg ccagcgtatt gcagcgcgcc atctcggcga gcgcgatgcg
     2221 gtgatagtgc tcgtagacgt tcctcgccgc gggcatgaaa tgagtcgcgt tgatggtgcc
     2281 gattgcttcg acgtagctat ggcagggcaa gttacccggg tcgaagccag gctggcccgg
     2341 aggtggaccg aaggcgcctt cgatgtcgtc caggaagcac acttcatttc cagccgccca
     2401 gtcgccgatc gataacgata aatccggaat ctgggcaagc tcgtaaaggg tgatgccgcg
     2461 taaagcacgc gtcccgggag ccccgaactt ctgcggcatc cagcgctgcc ccgcgatcca
     2521 gccctggccg ttcagcaact tagaggcaat gtagctgttc gggacggaat actgaaccca
     2581 gaaaggttca ccaagcctgc tcggcagccc ggcgcggcgt gctgcttcat gcgtcaacgc
     2641 cgcatgctcg ccgaggtccc cgcccgcgcc gaaccatgct ttacgtacgg ttacccacgg
     2701 caacccgggg tcggcctcgt cgtcgctag
//
LOCUS       DASTKU010000373         7505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_70971,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000373 DASTKU010000000
VERSION     DASTKU010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70971"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..968)
                     /locus_tag="VFQ61_12805"
     CDS             complement(93..968)
                     /locus_tag="VFQ61_12805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9955384.1"
                     /translation="MDRGYELALAFCAAAQLGEADPDLALIEAELQVRWEAARSAWPE
                     LELEIWDFVRHLGRASAAGVLPPLANTADLALALSCARGQTRAIAIFHRAYGEVISRV
                     LSRRGANAGLAEDAAQLVHERLLVGSAAVPPKIWEYRGQGPLRSWVSTAAATTLLQLR
                     RSADRRREHSVEEDNSGELVAELDPELGYLKERYKTHVHDAIVAALGQLGDRERTLLR
                     LHLGQRMSIDQLGAMYRVNRATAARWLAAARAFLVERARGELKTRLRISDSECNSIVA
                     LVRSQLDVSILQHLS"
     gene            1032..2585
                     /locus_tag="VFQ61_12810"
     CDS             1032..2585
                     /locus_tag="VFQ61_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9955385.1"
                     /translation="MAPEPAEECLDENTIATFVAGALESRALGAVEAHLARCVQCQRL
                     VADAAYGAYGNSASATLEPNIAASPSPDAMKSSARKPVPVPRTGQLVDGKYRIERVLG
                     RGGMGTVLAAFHQELGHRVAIKLLHHAGSSAATRLLREAQACAQLANDHIVRAFDFGR
                     LPDGTTFLVMEYLEGQDLSRLIAVGPVPYAEASRYVYEACAALVEAHAAGIVHRDLKP
                     ANLFLVTRANAKPTIKLLDFGISKLMSPEAQDAASTLTSTGALLGSPLYMSPEQIRAS
                     KDVDARTDIWSLGVILYELLTGRPPFMAPTLAALSVAIATDAAPRPSTRHAGLPAALD
                     EIVLRCLSKDRSARFPSAAALAAALKGFCDAKDVHPGASTPSAFPQWKRPTRKLALGA
                     GVVVVAAGVAIYRWSYPPSTRDSRPDLNASLPALTKDSPHTELRPSEQKQPGAQPQPS
                     EQPRSIDPNPARVTVTENAPLLESSAPLPSSKPASGTTRERPARPKSQARPRPVPSKP
                     KLDPIGSPD"
     gene            complement(2601..3584)
                     /locus_tag="VFQ61_12815"
     CDS             complement(2601..3584)
                     /locus_tag="VFQ61_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955386.1"
                     /translation="MADLITVAEAALRIRSGQLLMIAGEEALLAELPAGSWIGGTTPY
                     FMAPSGGRADTKRVFVTELSGPVRGGTINVYAHDELESIPNDYARQGCSFIILPTGSA
                     THFRFANCASSLPGLFNCPLVGWNAGVMLNSESRSPRVFDGRTRRCLDDAAVVLHAEL
                     AEDYVARIDIVNPFQQGQGPKIAFPRSGFTVTECWVDGREQSFCEFLAKHQADTRWPL
                     VADYAGAAINVSFRNIDRENNTVHLYAPVFEGVEYRLAEPVADLAEVLAREFNERREQ
                     PAFTCNCVLNYLYAELEGKSTGEAAGPITFGEVAYMQLNQTLVYVTFDRAA"
     gene            complement(3577..3861)
                     /locus_tag="VFQ61_12820"
     CDS             complement(3577..3861)
                     /locus_tag="VFQ61_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955387.1"
                     /translation="MSVAVEEDLDKRSMPISRATKVPPITDPRWSEFISGRRNYELKC
                     LASRIMYGQVKLLAQRDPPHAVRMAYEYFQRNEALAAEDLRLIFESTSHG"
     gene            complement(4183..5697)
                     /locus_tag="VFQ61_12825"
     CDS             complement(4183..5697)
                     /locus_tag="VFQ61_12825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9955388.1"
                     /translation="MVPSLPASRKVWSVPTIVFGLLLVCYATLVWVYGRSPEIAEDWH
                     GWRQADTQVIARHFAEPGSSLFLPRIDWGGSKAGYVEAELQLYPWVVAWFLRLFGDAE
                     WPGQFVSLVCTLAAVAVLQRGFARNFGPLAASLGALALLSSRSIAYTATALQPEAMCL
                     LFYAIAWTQFQRYEETRGRAHLIWFAVAGVLAMLVKPTAAQIGISSFLLLALRSRRHL
                     AKPDPWLAWAGMLAVFGAYLLVARSVYEQYGNSFGVLFGSTSKIPKLEHLARLESIKG
                     AAMQSVIWGLGPVGALALLVLTVRRRVDAVLIALLAGNIAWTLLAIRQTSGIAGTHYH
                     VLMSVVTAYATALAVHTLPARGVLRVGAAVAVAATLVLVLRQRSHLRMAMAPNVSAAA
                     QTLSRYAHPGELVVVRSMSPARSQQWGVAVNYQDPRIHFLSHTHGWVLPFDEHSPTTL
                     AQAVAEGASYYVEIERAPDLGPFDTWLAEHATQLGANSYGGRLYALKPRLVSGL"
     gene            5892..6611
                     /locus_tag="VFQ61_12830"
     CDS             5892..6611
                     /locus_tag="VFQ61_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955389.1"
                     /translation="MKISGIACIFSALLAACGSSSEGADVGALGAERSDAGSATFSKE
                     LRVGDASGQNQIRLRVTARDAQALASYFSGRFVLEVWSAAQSASAPESSPGGSREARP
                     EFPTLPDLAAHAIGSVQPAPSVRLDIIEERLATEVTGYRVLDTAAVDTAAPSYRAPFT
                     IRYHETALDCVSVQRVSEFQKVYASIQSQTNSGDPWLTIASEVTIKNGDTLESCDYNS
                     YKVRARVEARNTNDYVLDYHN"
     gene            6628..>7505
                     /locus_tag="VFQ61_12835"
     CDS             6628..>7505
                     /locus_tag="VFQ61_12835"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9955390.1"
                     /translation="MAASSVAARYQSAGVWMRALSLPRVSSRCSQGLPALLACAFQLA
                     CGAEVREDPHRYAAPIVRGMAAGDMLSSLVALIREEHTPDYSSLAVACSGVLIASDVV
                     LTAAHCLQDPGLEPPDPRAAGEAVWHVSFEPDLTQIGLQTFELPSDAVHVREAVLHPE
                     FSYLEPAPIGLGPNHDLALLRLAEHVTSRVPATVLPATLRAALHAQREVVIGGYGLQN
                     PDGETSEAPHDPPGLRAVGRSLILEVAEQELRVGAPDARMGAASFNEFADKCSGDSGG
                     PSLIQLGNTEYVVGIA"
BASE COUNT         1404 a         2475 c         2328 g         1298 t
ORIGIN      
        1 tgggaccgca ctggcgcctc gaagctctcc ggcttcatgt tgctgctcga tgacctcgcc
       61 ttcttgcctc ccgaggcgca gtaaccgtcc gctcaactca ggtgctgcaa aatcgagaca
      121 tcgagttggc tgcgcaccaa cgccacgatg ctgttgcatt cgctgtcgct gattcgaagt
      181 cgcgtcttga gctcgcctcg cgcacgttct accaggaaag cccgggccgc cgcgagccaa
      241 cgcgcggcgg tggcccgatt gacacggtac atggcgccta gttggtcgat gctcatgcgt
      301 tgcccgaggt gcagacgcaa cagagtccgc tcgcgatccc ccagctggcc gagcgccgcc
      361 acgatcgcgt cgtgaacgtg tgttttatat cgttccttca gatagccgag ctccgggtcc
      421 agctctgcaa ccagctctcc cgagttatcc tcttccaccg aatgttcgcg ccgccgatcg
      481 gccgagcgcc ggagctgcag caaggtcgta gccgcggccg tcgagaccca actgcgcaac
      541 ggcccctggc cacgatactc ccatattttg ggtggcacag ccgccgaccc cacgagcaag
      601 cgctcatgca ccagttgcgc cgcatcctcc gccaaccccg cgttcgcgcc acgtcgcgaa
      661 agtacgcgcg aaatcacttc gccataggca cgatgaaaga tggcgatagc tcgagtctgc
      721 ccgcgggcgc agctcagcgc caacgctaga tccgcggtgt tggccagcgg aggcagcacg
      781 cccgccgcgg acgcgcgccc gaggtgtcgc acgaaatccc agatctcgag ctctagctcg
      841 ggccacgcac tccgcgctgc ctcccaccga acctgcaatt cggcctcgat gagcgctaaa
      901 tcagggtctg cctcgccgag ctgcgcggcg gcgcagaaag cgagcgccag ctcgtaaccg
      961 cgatccatcc tgcggccaaa cgtagcacga acttgcgatg ggcgagatct cgggggcatc
     1021 cttgaggggc gatggcgccc gaacctgctg aggaatgcct cgacgaaaat accatcgcca
     1081 ctttcgtggc cggtgcgctg gagtcccgag cgctaggagc agtggaagcg cacttggcgc
     1141 gttgtgttca gtgtcagcga ttggtggctg acgcggccta tggcgcatac ggaaattcgg
     1201 cgtcggccac gctcgagccg aacatcgccg cctccccctc ccctgacgcc atgaagtcgt
     1261 cagctcgcaa accagtgccc gtaccccgca cgggccagct cgtggacggc aaataccgaa
     1321 tcgaacgggt gttgggacgc ggcggaatgg gcaccgtgct ggcggccttt caccaggagc
     1381 tcgggcatcg cgtcgcaatc aagctcctgc atcacgccgg ctcgtccgcg gccacgcgct
     1441 tgcttcgaga agctcaggcc tgcgcacaac tcgcgaacga tcacatcgtg cgagccttcg
     1501 atttcggccg gctgccggac ggcaccacct tcctcgtgat ggaatacctg gaggggcaag
     1561 atctgtcgcg cctgatcgcg gtcgggccgg tgccctatgc ggaggccagt cgctatgtgt
     1621 acgaagcttg tgcagcactg gtcgaggcgc acgccgcggg catcgtgcat cgcgacttga
     1681 agcctgccaa tctgttcctc gtaacgcggg ccaacgccaa acccaccatc aagctcttgg
     1741 acttcgggat atcgaagcta atgagtccgg aggcccagga tgccgcttcg acgctcactt
     1801 ccaccggcgc gttgctcggc tcgcctttgt atatgtctcc ggagcaaatc cgagctagca
     1861 aggacgtgga tgcccgaacg gacatttggt ctctcggcgt aatcctgtac gagcttctca
     1921 ccgggcggcc accgttcatg gcaccgacgc tcgcggccct gtcggtagcg atcgcgaccg
     1981 acgcggcgcc tcggccgtcg acacgacacg cggggctccc ggcggctctg gacgagattg
     2041 tacttcgctg cctgagcaag gacaggagcg cccgcttccc ttcggccgcg gcgctcgccg
     2101 ccgcgctgaa aggcttctgc gacgcgaagg acgtacatcc cggcgcgagc acgccgagcg
     2161 cgttcccgca gtggaagagg cctacgcgga agctcgccct cggtgctgga gtagtcgtag
     2221 tcgcggcggg cgtcgcgatc taccgctgga gttacccgcc cagcacccgg gattccagac
     2281 ccgacctgaa cgcttctctc ccggccctta ccaaagactc accgcacacc gagctacgac
     2341 ctagcgagca gaagcagccc ggtgcgcaac cccagccgag cgagcaaccc cgaagtatcg
     2401 atccgaaccc ggcgcgagtc acggtgacag agaacgcacc tctgctcgag agctccgctc
     2461 cgctcccgtc gagcaagccg gcctctggca ctacccgtga gcgcccggcg cgaccgaaaa
     2521 gccaagctcg gccacgcccc gtgccgtcca aacccaaact cgatccgatc gggagccccg
     2581 attgaaggcg gcgacgccgg ttacgccgcg cggtcgaacg tgacgtaaac cagcgtctgg
     2641 ttgagttgca tgtacgcaac ctccccgaac gtgatcggtc ctgccgcttc tcccgtactc
     2701 ttgccctcga gctccgcgta caagtagttc aggacgcagt tgcacgtaaa cgccggctgc
     2761 tcacgccgtt cattgaactc gcgcgccagg acctcggcca aatccgcgac gggctcggcg
     2821 agccgatact ccacgccctc gaacacggga gcgtagaggt gcacggtgtt gttctctcga
     2881 tcgatatttc ggaaactcac gttgatggcg gcccctgcgt agtctgccac caagggccaa
     2941 cgggtatcgg cctgatgctt ggcgagaaac tcgcaaaacg actgttctcg accgtccacc
     3001 cagcactcgg tcactgtgaa cccggagcgc ggaaacgcga tcttcggacc ctggccttgt
     3061 tgaaacgggt tcacgatatc gatccgcgcc acgtagtcct cggctagctc cgcgtgcagc
     3121 accaccgccg catcatccaa gcagcgacgc gtccgaccat cgaacacccg cggactgcga
     3181 ctctcggagt tcaacatcac accggcgttc cagcccacca aggggcaatt gaacaagcct
     3241 ggcaacgaag atgcgcaatt ggcgaagcga aagtgcgtcg cgctccccgt cggtagaatg
     3301 atgaatgagc agccttggcg cgcgtagtcg ttcgggatcg actccagctc gtcgtgggca
     3361 tagacgttga tggtcccgcc gcggaccggc ccagacaact ccgtcacgaa tacgcgcttc
     3421 gtgtccgccc ggcccccact tggggccatg aagtagggcg tcgtcccgcc gatccaggag
     3481 ccagcgggca gctccgcgag cagcgcctcc tcgcctgcga tcatcaacaa ctgccccgag
     3541 cgaatgcgga gcgccgcttc cgccaccgtg atcaaatcag ccatgggagg tgctctcgaa
     3601 gatcaagcgc aggtcctcgg cggcgagcgc ctcgttgcgc tgaaagtact cgtacgccat
     3661 gcgcacggcg tgtggcggat ctcgctgtgc gagcaacttc acctgcccgt acatgatgcg
     3721 tgaagccaga cacttgagct cgtagttgcg tctgccgctg atgaactcgg accaacgtgg
     3781 gtcggtgatg ggagggactt tggtggcgcg agagatcggc atcgaacgct tgtcgaggtc
     3841 ttcttcgacc gcaacggaca acgcatctgg ctcttaagct agagactccc gcgcctgctt
     3901 ctcgcctcgc ccgtctcgta tcgatatcac tcagcgggtt cctagtccgc gcgtctcgcc
     3961 ccgtttcgaa ttcccgtcta cacccgtcca catctcgctc tgacgcgggc ggccacgtcg
     4021 tcgacgtgcg cgaagcccgc cgcgctccct cgacggcgca gaagcgggct ccctctgccg
     4081 cgcgcgatac cgcgccggcc ccccaagtaa cgccgtcgca gctgcctcgc attgcgccgc
     4141 ccaagttcca ctgcgatcaa gcggcgcagc ctcgcttcga agctacaggc cgctgacgag
     4201 gcgaggcttc aaggcgtaaa gtcggccgcc gtaagagttg gcacccagtt gcgtcgcgtg
     4261 ctcggcgagc caagtatcga atgggccgag atccggagcg cgctcgatct cgacgtagta
     4321 ggacgcgccc tccgccaccg cttgggcgag agtggtgggg ctgtgctcgt cgaacggcag
     4381 aacccagccg tgcgtgtgcg agaggaagtg aatgcgcgga tcttggtagt tgacggccac
     4441 gccccattgt tggctccgcg cggggctcat cgaacgaacg acgacgagct cgccggggtg
     4501 agcataacga gacagggtct gggcggcggc ggagacgttg ggcgccatcg ccatgcgcag
     4561 atgtgagcgt tgacggagta cgagcaccaa cgtcgcggcc acggccacgg ccgcacccac
     4621 gcgcaacacg ccacgcgcgg ggagagtgtg gaccgcgagc gcggtggcgt acgcggtgac
     4681 cacggacatc aatacgtggt agtgcgtgcc ggcgatacct gacgtctgcc gaatggcaag
     4741 cagggtccag gcgatgttgc cggccaatag cgcgatgagc acggcgtcga cccgacgacg
     4801 caccgtgagc acgagcaacg ccaacgcacc caccggacct agcccccaaa tcaccgactg
     4861 catcgcggca cccttgatgc tctcgaggcg ggcgaggtgc tcgagcttcg ggatcttcga
     4921 ggtgctgccg aacaacacgc cgaaggagtt accgtattgc tcgtacacgc tccgggccac
     4981 caacaaatac gcgccgaaga cggcgagcat gccggcccac gccaaccatg gatcgggctt
     5041 ggcgaggtgt cgccgggacc gcagcgcgag cagcaagaag ctcgagatac cgatttgcgc
     5101 tgcggtgggc ttcaccagca tcgccaagac cccggcgact gcaaaccaga tcagatgcgc
     5161 gcgcccacgc gtctcttcgt agcgctggaa ttgggtccaa gcgattgcgt agaacagcaa
     5221 acacatggcc tcgggctgga gcgcagtagc ggtgtacgcg attgaacgcg aactcagcag
     5281 agccagggcg cccaagctgg ccgcgagcgg cccgaagttt cgagcaaacc cacgctgcag
     5341 caccgcaacc gcggccaagg tacagaccag agaaacgaac tgacccggcc actctgcgtc
     5401 tccgaacagt cgcaaaaacc aagctacgac ccaaggatac agctgcagct ccgcctccac
     5461 gtagcccgct ttgctaccgc cccagtcgat gcggggcagg aacaagctcg agcccggctc
     5521 ggcaaagtgc ctggcaatca cctgggtatc tgcttgacgc caaccgtgcc agtcttcggc
     5581 gatttctgga gagcgcccgt acacccaaac cagggtggcg taacagacga gcagcaatcc
     5641 aaacacgatg gtcgggacac tccacacttt ccggcttgcg ggcaaactcg gcaccattgt
     5701 cgggcacctt actgaactca acccgggtgt gggaagccgt gaaagcctcg acaacaatcc
     5761 tactatgggg cctgcgggtt ttgttttggg ccacggatct cgagccgcgc tgccgggcgt
     5821 gtgcgaggtg aacgcgcggc gcggggtgga cccccgcgag tgaaaccgcg cctcgattca
     5881 ggagaattgt catgaagatt tcgggcattg catgcatctt ttccgcgtta ttggcggcat
     5941 gtggatcgag ctccgaaggg gctgatgttg gagcgctcgg tgcggagcga tcggacgctg
     6001 ggtcagccac cttctccaag gagctccgcg tgggtgacgc gagcgggcaa aaccaaatcc
     6061 gattgagagt tacggcgcga gatgcgcaag ccttggccag ctacttctcg gggcgtttcg
     6121 tgctcgaagt gtggtcggcg gcgcaatccg cgtccgcacc ggaaagctcc cctggcgggt
     6181 cgcgcgaggc tcgcccagaa ttcccgaccc ttccggattt ggctgcgcac gcgattgggt
     6241 cggttcaacc agcgccatcg gtacggctgg atatcatcga ggagcgtctc gccaccgagg
     6301 tcactggata tcgagtgctg gatacggccg ccgtagacac ggccgccccc tcgtatcggg
     6361 ctccgttcac gattcgctat cacgagactg cgttggactg tgtctcggtg cagcgcgtct
     6421 cggagttcca gaaagtctac gcatcgattc aatcccaaac caactccgga gatccttggc
     6481 tgacgattgc aagcgaagtg accatcaaga acggagacac tctggaatcg tgtgactaca
     6541 acagttacaa ggttagggct cgagtcgagg cacgcaatac caacgactac gtgctcgact
     6601 atcacaactg agtcgtgagc gtagcgcgtg gccgcttcat cggtggccgc gcgctaccaa
     6661 tccgctggtg tttggatgcg cgcgctctct cttcctcggg tctcgtcccg ctgctcgcaa
     6721 gggctgcccg cgctcctggc ctgcgcattt cagttggcgt gcggagcgga ggttcgtgag
     6781 gatccgcacc gctatgcggc gccgatcgtg cgcgggatgg ctgcgggtga catgctatcg
     6841 tcactcgtgg ccttgattcg tgaggagcac actcccgact attcgagttt ggcggtagct
     6901 tgcagcggtg tgttgatcgc ctcggacgtg gtgttgaccg ccgcgcattg cctgcaagac
     6961 cccgggttgg agccacccga cccgcgcgcc gcgggcgaag cggtgtggca cgtcagcttc
     7021 gagccggacc tgacccaaat tggtttgcaa accttcgagt taccttccga cgccgtgcat
     7081 gtgcgcgaag ccgtcttgca cccggaattt tcgtatttgg agccggcacc catcggcttg
     7141 ggaccgaatc acgatttggc cctgctcaga ctcgccgagc acgttacttc gcgggtgccc
     7201 gcgaccgtgc tcccggccac gctcagggct gcccttcacg cgcagcgtga ggtggtgatt
     7261 gggggatacg gcttgcagaa cccagacgga gagaccagtg aagctccgca tgacccgccc
     7321 ggtctgcgcg ccgtcggcag gagcttgatc ttggaggtcg cggagcaaga gttgcgcgtg
     7381 ggtgcgcccg atgcgcgaat gggggccgct tcattcaacg agttcgctga caagtgcagc
     7441 ggtgactctg gagggccaag cctgatccag ctcggcaata cggagtacgt ggtgggcatc
     7501 gcgag
//
LOCUS       DASTKU010000374         4753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_739228, whole genome shotgun sequence.
ACCESSION   DASTKU010000374 DASTKU010000000
VERSION     DASTKU010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739228"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VFQ61_12840"
     CDS             complement(<1..645)
                     /locus_tag="VFQ61_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955391.1"
                     /translation="MNSPRSNLGRGRWVFLVLSLVGSPLLAHCSSELGISPTESLETR
                     SSGLGAFNVLTRSYNNQRTGANLSETLLNQSNVNARQFGKLFQLPVDDEVYAQVLYAS
                     GVTINGAARNVIYVATVNNTVYAFDADAPGAPLWQRNFNGAGRAPRNYEVANGTACSR
                     YYRDFAGNIGIVGTPVIEPVSGTLFFVTRTLESEQMRYYLRAVSIVSGAERSGSP"
     gene            complement(1134..1679)
                     /locus_tag="VFQ61_12845"
     CDS             complement(1134..1679)
                     /locus_tag="VFQ61_12845"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9955392.1"
                     /translation="MAGISVAGSHGRRSLDLDLPLVPFIDFLLCLIAFLLVTAVWSQM
                     ARLNADARVPGRQDTVIDPSKERQLHVEVKGSEPFKLVWKQGNTVLNTFDVERRAVPF
                     GSGGDIRYPELAKVIEREWLQNGAHRTPADLKLDQAVLHTDNSAPFNELVAVMDAIYT
                     PKRTSSSREPTPAFNVTFAAD"
     gene            complement(1985..2680)
                     /locus_tag="VFQ61_12850"
     CDS             complement(1985..2680)
                     /locus_tag="VFQ61_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955393.1"
                     /translation="MQILDTLGSVIRLPPPPLWYVTNGDLTVGPVPTNLLIRGIEAGR
                     VPNFCEVKAGAGRWRGITAVREVAALYGKAKTPSEQFNEWSRSAEYIKDEAEFAHTLT
                     WLSALATSAENAMFHYRANFGRSLITRSVLGPMAADRLGYPLSEHDLVLKTARLARPI
                     FGPPFGPLEDALAKRFASSEGGSGAVAMIPFHIEGQLIAMLELSRPGHAFRRTDLQRA
                     ERIIKRALRKRHS"
     gene            3346..3951
                     /locus_tag="VFQ61_12855"
     CDS             3346..3951
                     /locus_tag="VFQ61_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955394.1"
                     /translation="MPLITLLGAGLLASAAQTAPAAVLGPAPTMQSDRVSTLASTDSK
                     NSCTPAPDRRAYFSARLALGLSSLKIYSVPISSREISLGLGARSGAQAAFFSFTWNQG
                     ETQFGLDTKSWHLALGGETSYSRLRGGAELRFGRMIIQSLTRDENLSSLSIGPALYAQ
                     LDVIRLRHTDALYLEAAGRADYYSPWVFEGSVKLGYRFSND"
     gene            complement(4012..>4753)
                     /locus_tag="VFQ61_12860"
     CDS             complement(4012..>4753)
                     /locus_tag="VFQ61_12860"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9955395.1"
                     /translation="ANRRSSPVQAVRTAITLLGLKRVASIAVGVACRSLFDVEVRVQH
                     ELLPGWWERLFHCAMTEAFAASFVAMEHNRAASDGIFLAAMLHDIGKSLALRSLAALT
                     ISGQVQALPEDETIEELLRRLRVPVGVAALTALNMPENLVVLCGHQDDEDLPETKEWT
                     DVHVVRLVSALNDLRLATLNTEQPIRFLLGAVRSMHLSPDAVVAIARQVSEHAAQVGL
                     LFSTTDGAEEIGYLDFVARCVQQANEKS"
BASE COUNT          935 a         1429 c         1481 g          908 t
ORIGIN      
        1 cgggctgccc gaacgctcgg cgccactcac tatgctgacg gcgcggaggt aatatcgcat
       61 ctgctcgctc tcgagagtgc gagtcacgaa gaacagggtg ccggagaccg gctcgattac
      121 gggggtgcca acgatgccga tgtttccggc gaaatctcgg tagtatcggc tgcaggcagt
      181 gccgttggcg acctcgtagt tgcgcggtgc gcgacctgcg ccattgaagt ttctctgcca
      241 gagcggagcg cccggcgcgt cggcatcgaa ggcataaacc gtgttgttga cggtcgcgac
      301 gtagatcacg ttgcgggccg cgccgttgat ggtcaccccc gaggcataca aaacttgggc
      361 ataaacctcg tcgtccaccg gcagctgaaa cagcttcccg aactggcgag cgttgacgtt
      421 gctttggttc agcagcgttt cactcaggtt tgcgccggtg cgctggttat tgtagctgcg
      481 cgtcaggaca ttgaacgcgc cgaggcccga gctgcgcgtt tcaagtgact ccgtgggtga
      541 aatccccaac tccgagctgc aatgggcgag gaggggtgag ccaactaggg ataacaccag
      601 gaacacccat cgaccccgac cgagattgct gcgcggcgaa ttcatgctgc ctccgaaccc
      661 gccaatttac atttcggctc atgtccgtca atcgggcaga tgatgcgccg acagcccaca
      721 agcaaatcat gattgctgcg tggaatcacg cagcaggttc acgcagcagg ttcacgcagc
      781 ggtatcacgc agcggtatca cccagcgcta tcagctcaat cacccggtaa gcgtacactc
      841 gctatgcgga tgagcgatat agagtcattc tgcggcgggc tgaacgctgc gggcgttcgt
      901 cttgtccatc ctgtgctcgt ctagctcgaa agcggatgaa cgaaggcgcg ctttggcctg
      961 ccccaccccg cgtttgggga gccaagtgag agcgagcaga tctagaaaca gtgcggacag
     1021 gggttcggag tacactgtgt ggcggacgga gcccagcgaa gaggcaccgt ccgccagaga
     1081 tacgtcacgc gcccgtgggg caataaggct cgggcaacgg acctcaccgc gtctcaatct
     1141 gcggcgaagg tcacattgaa ggcgggtgtt ggctcacggc tcgagctggt gcgcttcggt
     1201 gtgtaaatcg cgtccatcac tgcgaccagt tcgttgaaag gcgccgagtt gtccgtatgc
     1261 agcaccgcct gatcgagttt gaggtcagca ggtgtgcggt gtgcgccgtt ttggagccac
     1321 tcgcgctcga tgaccttcgc caattccgga taacgaatgt ctcccccgct accgaacgga
     1381 acggcgcgcc gctccacatc gaaggtattc aaaacagtat tgccttgctt ccacaccagc
     1441 ttgaagggct ccgagccctt cacctcgacg tgcagctgtc tttccttact cggatcgatc
     1501 accgtgtcct ggcgtccggg cactcgcgcg tcggcattga gccgcgccat ctgcgaccac
     1561 acggccgtga ccagcaggaa ggcgattaga caaagcagaa aatcgatgaa tggaacgagt
     1621 ggaagatcca gatcgagcga tcgtcgaccg tgacttccag cgactgagat cccagccatt
     1681 tttcacctcg ctagctcgaa gccacttggt ggttggcttc ggcgtgccga aagacacggg
     1741 gcgaggggca atcattggct cacgacgtca gaaaacgtga gccctatgaa cgccattcga
     1801 gacctcggct ttttgctcgt ggtcgggaac tttggcaatc gagcgttgtc gatgcgcggt
     1861 cgagaacggc gcaacctcga agccgattca cgaagcgagc ttatcgcgag tcggcggcgt
     1921 tcgaagactg ggaacatcga gtcaccgcac gtcgcgtagc ccgcgtcgaa ggatgctccg
     1981 caggctagct gtgccgtttt ctcaaggcgc gcttgatgat ccgctcggcc cgctgcaggt
     2041 ccgtacggcg gaacgcatga ccggggcgcg agagttctag catcgcgatg agttgaccct
     2101 ctatgtggaa ggggatcatc gcaaccgcgc cgctgccgcc ttcactgctc gcgaagcgtt
     2161 tggccaaggc gtcttccaga ggaccgaatg gcggcccgaa gatcgggcgc gccagccgag
     2221 ccgtcttcaa cacgagatcg tgctccgaaa gcgggtaacc caaacgatcc gcagccattg
     2281 ggcccagcac cgagcgagtg atgagtgagc ggccaaaatt ggctcgatag tggaacatgg
     2341 cattctcggc gctcgtggcg agtgccgaga gccaggtgag cgtgtgcgcg aattcggcct
     2401 cgtctttgat gtactcggcg gaccggctcc actcgttgaa ctgctcggag ggggtcttcg
     2461 ctttgccgta cagggcggcc acttctctga cggcagttat cccgcgccag cggcctgctc
     2521 cggctttgac ttcgcagaag tttggaacgc ggccggcttc gatgccgcga atcaataagt
     2581 tcgttggaac cgggccgacc gtgaggtccc cgttcgttac gtaccaaagg ggagggggcg
     2641 ggagccggat gacgctgccg agcgtgtcca gaatctgcat ggcgggggat tctcaatcga
     2701 gcacccacgg ctggccaaca aaccggtggc gccgaacacg aggctgcgca attggctcgg
     2761 aacgcgcgga tggggggcta tgcaagcggt tccaactcgc ggaccgaagc gccgagtggc
     2821 gcccccaagg cctcgaaccc tgagttttgc tggcacaggt ccgtgctcga cactgtgagc
     2881 ctgagactac accgtcggcg ggatttggcc tgatttcctc acgaaacttt cgttcgaccg
     2941 aaaacgcgcc gcgggcattc agcttgcaaa ctcggcgaac atgtttgcgg gaatgcttgc
     3001 cagcacgctc cggcgtcggg tcccggacgg cgttagggcc gttttacttg gcggcaccat
     3061 cggactctta ttgagccacg gtcactcgct aggctttcga caagacgaac tcgaatgtga
     3121 ggaggcggta gctcgcctac aggaatgctg ctctggtctc gacgtgagca gattcgactg
     3181 tgacttcacg cccgcaggtt gtgagagcga ggcgagctat ccggaaattc acgaagacca
     3241 gagcgcgtgt gttcgaggtc tatcctgcgc agctattcgg gagagcggag tttgcgagcg
     3301 tgcggcggaa aacaaaccgt actccagggg tggaggcctc gagctatgcc tttgataact
     3361 ctactcggtg cgggcctgct cgcgagtgcc gcgcaaaccg cgcccgcagc ggtgctcggc
     3421 cccgcgccga cgatgcagtc cgatcgagtc tccactctcg ccagtacaga ttcaaagaac
     3481 tcatgcacgc ccgcgccgga tcgacgagct tacttctcgg cgcgcctcgc cctcggcttg
     3541 agttccctca aaatctacag cgttccgatt tccagtcgcg aaatcagcct cggtttgggg
     3601 gcccgttcgg gagcccaagc ggccttcttc agtttcactt ggaaccaggg cgagacccaa
     3661 ttcggactcg acaccaaaag ctggcacctc gccctcggtg gagaaaccag ctacagccgt
     3721 ctgcgcggcg gagccgagct ccgcttcggc cgcatgatca tccagagcct cacccgagac
     3781 gagaatctaa gctcgctttc gatcggcccg gccctctacg cgcagctcga cgtgattcgc
     3841 ctgcgccata ccgacgccct ctacctggaa gcggccggaa gagcggatta ctactctccc
     3901 tgggtgttcg aggggagcgt gaagctcgga tatcgattct cgaacgactg atggtcgaaa
     3961 gccttccctc gccgggactc gccagcgctg ggtcgggcgc gtttctcggt ctcagctctt
     4021 ttcgttggct tgttgaacgc agcgcgctac gaagtctaga tagccaatct cttcggcgcc
     4081 gtcggtcgtg gaaaacagca gtccgacctg cgcggcgtgt tcgctcacct gtcgggctat
     4141 cgccaccacc gcgtcaggac tcaagtgcat cgaacgcacg gccccgagta agaaacgaat
     4201 gggttgctca gtgttgagtg tggccaagcg caggtcattt agggcggata ccagacgcac
     4261 cacatgaaca tcggtccatt ccttcgtctc gggcaggtct tcgtcgtctt ggtgaccgca
     4321 cagcacgact aggttctccg gcatattcaa cgcagtgagc gccgcgaccc caacgggaac
     4381 acgtaagcgg cgcaagagct cttcgatcgt ttcgtcttcg gggagcgcct gcacttgccc
     4441 cgatatggtg agcgcggcga gggaccggag cgcgagcgat ttgccgatgt cgtgcaacat
     4501 ggccgccaaa aaaatgccgt cggaagcggc acggttgtgc tccatggcca cgaaggaggc
     4561 cgcgaatgcc tcggtcatgg cgcaatgaaa caggcgttcc caccagcccg gcaggagctc
     4621 gtgctgaacg cgaacctcga cgtcgaaaag gctgcgacat gccacgccga ccgcgatgct
     4681 cgccacgcgc ttcaagccga gtaacgtgat ggccgtgcgt acggcttgaa ccggggaaga
     4741 gcggcgattg gcg
//
LOCUS       DASTKU010000375         9363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1632191, whole genome shotgun sequence.
ACCESSION   DASTKU010000375 DASTKU010000000
VERSION     DASTKU010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9363
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632191"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFQ61_12865"
     CDS             <1..582
                     /locus_tag="VFQ61_12865"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET9955396.1"
                     /translation="LRLEMRAEIRRIHRELGRTTIYVTHDQDEALSLSDRIVVMRQGI
                     AQQIAPPKVVFEMPSNLHVARFMGYRNVLDLDVAARESQRARVLGPGIELWGTVAEPF
                     TGTRASVAIRPDEMTVVAPGSPNSLAATVVSSEYGGRDSLVEVLAASGTRLYVRTSQH
                     VNPGDTISISAPPERVLVYPVEAPATTAPVRPN"
     gene            590..2341
                     /locus_tag="VFQ61_12870"
     CDS             590..2341
                     /locus_tag="VFQ61_12870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9955397.1"
                     /translation="MMSPTSTAGLPNAATARERAFRVDPGVVLVLPATLFLLSLFVYP
                     FLYGLVLSLQPNQGGVFENYIKFFSGQQWVTILTTLKLALPATLINTGLSLPIAYRLR
                     AKTFYQRFVTTSLVVPVALGTVLIAEGMLTYFGPRGWVARTLEFLHLAESVRLTHNYW
                     GVLISLVISGFPFVFLLTLSYITGIDPGLARAASTLGASPWKQFKEIYLPLVIPGLEM
                     SFCLAFVQAFSVFPSAVLLGAPAGPTRVISIEAYEAAFERYDYSMASAIALIMGFCQL
                     MIVAPMLGLRSLSYRGPASGGAGMSAPPAEQVDRPWYERAVGVGLLGLVAFFVLNVAL
                     MSLAVLINSFGKRWLGTWFPEGFTTHWYAEAWNEFRLGEVLGVTVEVVLAVVFLSLAI
                     GVPAAYSLARRDFRGKRPLLLLFLLPLMVPPITYGIPLATVMYKVQLAGTLWGVILAN
                     TIPALPFVIMVMVPFIEQIDPRLESAARVLGASTGRVFRHVLLPLLAPGMLAAALLVM
                     VRTIAMFELTFLTAGPDSQTLVVALYYAVFAAGVRASQSVDAMAMVYMAFTSLWLIVA
                     LCFVNPTQMVSRAREQQ"
     gene            2649..3614
                     /locus_tag="VFQ61_12875"
     CDS             2649..3614
                     /locus_tag="VFQ61_12875"
                     /inference="COORDINATES: protein motif:HMM:NF021454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HET9955398.1"
                     /translation="MRSISQARAVRWWGLWSACWLAVMLFGGSLAWAQGSPPAADAKA
                     EPAEQQPETLPAPWESGPKAVSLGHEVTVDLPAGYAYLPPEPARKVLERNGSFHNDDV
                     LGLLASTDPQQEWFVVTSFEESGFIKDDEKLDGDELLSSMRDGLDEMNEERKKHGFKP
                     LALDGWGEPPRYDQGRHHLVWALIVSDPEGKSVNLNTRVLGRRGYVSLNLVTDPKNLA
                     QYRHHGTALLTATHFANGARYEDFNEKTDKMAEYGLAGLIMAGAGLGAAKLVKLGLLA
                     KFSKVIIGVLIAGKKVIVVAVLALAGIAKRLFSGRRAETPPSPGA"
     gene            3711..5159
                     /locus_tag="VFQ61_12880"
     CDS             3711..5159
                     /locus_tag="VFQ61_12880"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9955399.1"
                     /translation="MSQPYEPGSVLAGKYRVERILGQGGMGIVVAALHLSLDELVAIK
                     FLLPEALHNPQVVGRFEREAKAAAKIRSEHVARVRDVGKLDWGTPYMVMEYLEGRDLG
                     QILRQRGCFPLQEAVDYLLQACEALAEAHALGIVHRDLKPSNLFLTARADGSACIKVL
                     DFGISKVLGAGSGNYAEMGMTRSTMVLGSPLYMSPEQLSSSRDVDTRTDIWSLGVILF
                     ELLTGRPPFAAQSLPQLCMTIMTQGAPNMRETRPDLPIGVEQIVAQCLQREPKQRIQS
                     VAVLAQMLTQFAPKRSMLSVERIARVAQSSGMTALGSNDAVVERPFGLDGTLAEFGRT
                     TRDRRTSQRNRMLAGVSLLALGAVGGGYVWFNRGLARLESHVSGASSAPALETHANSG
                     RVVLTAPVPTAAPPAAAAPAPTASAPPVEAVSSTPAPLPPPAASVEGPRASGAAKRVS
                     ESSPAKPKTDKGAAPTGSSKAPNNNSPWGGRL"
     gene            5254..6303
                     /locus_tag="VFQ61_12885"
     CDS             5254..6303
                     /locus_tag="VFQ61_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9955400.1"
                     /translation="MNRLFLTCAALSSVLGVTLAHAEADRGAAVVLFDQAQALIESGK
                     FSEACPKFAESYRLDPQLGVLLHLAECYERTGRFASAWVAFRDAIELAERRSDGRAAA
                     AREHASALEPKLSHLSVVVPSAALVDGLKVQRDGIELSPALWGSPLPVDPGEHVIEVS
                     APGKQSFRSTVSVKDPGEVASVNVPVLSDLASKNEAPTALDVNVASPPRSSFLSPPTR
                     TWLGIGALGLGATSAIVGIVFHAQRASKIDDRDAICPSSMNCTRDEANRIDQLTDEAR
                     SAATIGTVGLAIGGAFIATGAVLLLTAPRDGGAPREAAIGRGLTLSPVFSPALGHAGS
                     APRATLQGLVMRGSF"
     gene            complement(6350..7228)
                     /locus_tag="VFQ61_12890"
     CDS             complement(6350..7228)
                     /locus_tag="VFQ61_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955401.1"
                     /translation="MHDANQPPCPEPTSASKRGRLRRASGLALLLVMAAGVAFVAGRS
                     SAAGVPLKQPLTYSGSLFDDSGQPLSGKHEIVLRLLPTDSDDASPLCETTAQIEPIGG
                     QFDVSLNAKCTAAFQNSPDVWLEAIVDSESMGRTKTGVVPYALEAQRAKVADLASAAT
                     DDLAKELDSIRTRLDGLSCPKNYYQAGPHLCVESGALHAPAAPWDAIGACSAQGDHLC
                     SQMEMQQVCASRGVAGSTVPADFNPYSGEARGMYSDHAADDTYVTWNVGACNANNDGL
                     PVAVLDGVPLPFRCCR"
     gene            complement(7249..7752)
                     /locus_tag="VFQ61_12895"
     CDS             complement(7249..7752)
                     /locus_tag="VFQ61_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955402.1"
                     /translation="MAFRSLFLLLAGFPVLGSACVSDRKVSIIDETASGGATAEGGRS
                     QGGRTSTNTNTNASGGDSSAQGGSAGAGGASCETGAARCNGNTPEHCEEGEWLPRAEC
                     EGDRPICQNGVCSNMRSSGHLSWQSPAPVTSGQPYRLLHHGISGDKALHAKVGGQWFT
                     LRGALHP"
     gene            8212..>9363
                     /locus_tag="VFQ61_12900"
     CDS             8212..>9363
                     /locus_tag="VFQ61_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955403.1"
                     /translation="MWDRVFRSTNNGWSLSLAVGLIAGYLGCSASDSTSTRGGPNASH
                     SFGGSKGQDPPVVLPNPDPPPEQEREAAYRLPVLSGRWIFAANPESGWVAAIDSRDAS
                     VALSRAGAQPRYLAALPPADGASARALVLDPGTAQARELRLTQTGLKPSQPLQTHRGA
                     NALSVSEDGGWALIWSDARQLEALDPVDSLQDVTLLAFGSASDEPPASTRLSVGYRPK
                     QVIFERGKRRLFVVCERTISIVELGAEPYVDRDVSLPVAAGQAPTDISLTPDGTLALY
                     RVEGQTEVHWLHLEDLTHGVVAFRNPVTDLDLMQDGLRAVVVARGRSIVRQTSADPAG
                     GASNAEVGGASGASGVVALVGGEAGASSGGVGGEPITSGGASGEFPLEVE"
BASE COUNT         1490 a         2782 c         3279 g         1812 t
ORIGIN      
        1 ctgcgcctcg aaatgcgcgc cgagatcagg cgcattcatc gcgagctggg tcgcaccacg
       61 atatacgtga ctcacgatca ggatgaggcc ctctcgttat cggatcgcat tgtcgtaatg
      121 cggcagggta tcgcgcagca gatcgcgccg cccaaggtcg tattcgagat gccgtcgaac
      181 ctgcacgtgg cgcgcttcat gggatatcgc aacgtgctcg acttggacgt cgcggcgcgc
      241 gagagccagc gggcgcgggt gttgggcccc ggtatcgaac tctggggtac ggtcgcagag
      301 ccgttcacgg gaacgagggc atccgtggcg atacggcccg acgagatgac cgtcgtggca
      361 ccggggtcgc ccaacagtct ggcggcaacc gtggtctctt cggagtacgg cggtagggac
      421 tcgctcgtag aggtgctcgc cgcgagtggg acgcgtctgt acgtgcgcac gagccagcac
      481 gtgaatcccg gggatacgat ttcgatctcg gcgccgcccg agcgggtgct cgtctatccc
      541 gtcgaggctc ctgcaacgac cgcccccgtt cgccccaact aggctgccca tgatgtctcc
      601 cacgtccacc gctgggttac ccaacgcggc cacggcacgc gaacgcgcgt tccgcgtcga
      661 tcccggagtg gtgctggtct tgccagccac tctgttcctg ttgagcctgt tcgtataccc
      721 gttcctgtac gggctggtgc tctcgttgca gcccaaccag ggcggtgttt tcgagaacta
      781 catcaagttc ttcagcggcc agcagtgggt gacgatactc accacgctga agctcgcgtt
      841 gcccgcgacc ttgatcaaca cgggcttgtc tttgcccatt gcttatcggc tgcgggcgaa
      901 gactttttat cagcgcttcg taaccacgtc cctcgtggtg ccggtggctt tgggtacggt
      961 gctgatcgcg gaaggcatgc tgacgtactt cggtccgcgg ggttgggtcg cgcggacgct
     1021 cgagtttcta cacttagcgg agagcgtacg gctcacgcac aactattggg gcgtgctgat
     1081 ttcgctggtg atctcaggct tcccgttcgt tttcttgctc acgctctcgt acatcacggg
     1141 catcgatcca gggttggctc gcgcggcttc tacattggga gccagcccct ggaagcagtt
     1201 caaagagatt tatctcccgc tggtcatacc tgggctcgag atgtccttct gcctggcatt
     1261 cgtgcaggcg ttctcggtgt tcccgtcggc ggtgttgctc ggcgctcccg ccgggcccac
     1321 gcgtgtgatt tccatcgaag cttacgaggc ggctttcgaa cgttacgatt actcgatggc
     1381 ctcggcgatc gcgctgatca tgggcttctg tcagctcatg atcgtggcgc cgatgttggg
     1441 gctcaggagt ttgagctacc gcggcccggc ttcgggtgga gcgggcatga gtgcgccgcc
     1501 tgccgagcag gtggaccggc cctggtacga gcgcgccgtc ggcgtgggcc tactcggctt
     1561 ggtcgccttc ttcgtgctca acgtcgcgtt gatgagcttg gccgtgctga tcaactcgtt
     1621 cggcaagcgt tggctcggga cctggttccc ggagggcttc accacccact ggtatgccga
     1681 agcctggaac gagtttcggt tgggtgaggt gctcggcgtt accgtagagg tggtgctggc
     1741 ggtagtgttc ctctccctcg cgattggtgt gcccgccgcg tacagcctcg cgcggcgcga
     1801 cttccgcggt aagcgcccct tgctgctgtt gttcttgctg ccgttgatgg tgccgcccat
     1861 cacgtacggc atcccgctcg cgacggtgat gtacaaggtg cagctcgcgg gcactttgtg
     1921 gggcgtgatt ctggccaaca cgatcccggc tttgccgttc gtgatcatgg tgatggtgcc
     1981 gttcatcgag cagatcgatc cgcgcttgga atcggcggcg cgggtgctcg gagcgtccac
     2041 ggggcgcgtg ttccggcacg tgctgttgcc cttactcgcg ccgggtatgt tggcggcggc
     2101 gttgctcgtc atggtgcgca cgattgcgat gttcgagctc acgttcctga cggccgggcc
     2161 cgactcgcag acgttggtcg tcgcgctcta ctatgcggtg ttcgcggccg gcgtacgcgc
     2221 gtcgcaatcg gtggatgcga tggcgatggt gtacatggcg ttcacgtcgc tctggttgat
     2281 cgtggcactg tgtttcgtga acccaactca gatggtgtcg cgcgcgcgcg agcaacagtg
     2341 aaggcggcgt ccaacgcacg cagctcttga tgcagcgggt ggataatcgc atataaaggt
     2401 gcgcgcgcct cggcacggcc gttcaggggt gccgcttgcg cggtggtgtc gggcgtgagc
     2461 gggcactacg gctgcgctcg cgggggttct gactggtgtc gcgcccgcta attcgggcgg
     2521 cctgctcata gtcattctcg atcgggatcc gagggccaat cggccgttgg caccgaatga
     2581 gcgctgcggt agtgtggccg agagcggctg gtgggtcgcg ggaaggttgg tgtgcgttga
     2641 agttccaggt gaggtcgata tcgcaagctc gggccgtgcg ttggtggggg ctctggtcgg
     2701 cgtgctggct ggcggtgatg ttgttcggtg gttcgctcgc ttgggcacag ggcagccccc
     2761 cggcggccga cgcaaaggcc gagcctgccg agcaacagcc ggagacgttg ccggcgcctt
     2821 gggagagtgg gccgaaagcg gtgtcgctgg gccacgaggt gaccgtggat ttgccggcgg
     2881 gctacgcgta cttgccgccg gaaccggcac gtaaggtttt ggagcgtaac ggctcgtttc
     2941 acaatgacga cgtgctcggg ctgctggcga gcacggatcc gcagcaagaa tggttcgtgg
     3001 tgacgagctt cgaggaatcg gggttcatca aggacgatga gaagctcgat ggggacgagt
     3061 tgctttcgtc catgcgcgac gggctggacg agatgaacga ggagcgcaag aagcatggct
     3121 tcaagccgct cgcgctggac ggctgggggg agccgccgcg ttacgatcaa ggtcggcacc
     3181 acctggtgtg ggcgttgatc gtgtcggatc cggaaggcaa gtcggtgaac ctgaataccc
     3241 gagttttggg gcgccgcggc tacgtctctc tgaacttggt gactgatccc aagaacttgg
     3301 cccaataccg gcaccacggg accgcgttgc tcaccgcgac gcacttcgct aacggggcgc
     3361 gttacgagga cttcaacgag aagaccgaca agatggcgga gtatgggctg gcgggattga
     3421 tcatggccgg cgcggggctc ggggcggcaa agctcgtgaa gctgggcctg ctcgccaagt
     3481 tctcgaaggt catcattggg gtcctgattg cgggcaagaa ggtgatcgtg gtcgcggtgc
     3541 tggctttggc cggcatcgca aaacggctgt tttcggggcg gcgagcggag acgccgccga
     3601 gcccgggtgc ctgacggcgg gcagccgatt cggctctggc gaaaacggga aaggcctgaa
     3661 cggaaatcgc ggctgggcga gcgcggatga gctggtaatc ttggttggcg atgagtcagc
     3721 cctatgagcc aggatcggtg cttgccggca agtaccgcgt tgagcgcatc ctgggtcagg
     3781 ggggcatggg gatcgtagtc gcggcgctcc atttgtctct ggacgagctg gtcgcaatca
     3841 agttcctgct gccggaggcg ctccacaatc cgcaggtggt ggggcgcttc gagcgggagg
     3901 cgaaagcggc ggcgaagatc cgcagcgagc acgtggcgcg cgtacgagac gtgggcaagc
     3961 tcgactgggg cacgccttac atggtcatgg agtatctcga ggggcgagac ctcggccaga
     4021 tcttgcggca acgcggctgt ttcccactgc aagaggcggt cgattacctg ctgcaagctt
     4081 gcgaagcgct ggcggaggcg cacgcgctcg ggatcgtgca ccgtgatctc aagccctcga
     4141 acttgtttct gaccgcgcgc gccgatggct cggcctgcat caaggttttg gattttggga
     4201 tttcgaaggt gctcggggca ggcagcggca actatgccga gatggggatg acccgctcga
     4261 cgatggtgtt gggctcgccg ctctacatgt cacccgagca gctctcgtct tcacgcgatg
     4321 tagatacgcg cacggatatt tggtcgctcg gggtgattct gttcgagttg ctcaccgggc
     4381 ggccgccttt tgcggcgcag agcttgccgc agctgtgcat gacgatcatg acgcagggcg
     4441 caccgaatat gcgcgagacg cggcccgact tgcccatcgg cgtcgagcag atcgttgctc
     4501 aatgcctgca acgcgagccc aagcaacgca tccagtcggt ggcggtgctg gcgcagatgc
     4561 tgacgcagtt cgcgccgaaa cggtcgatgc tctcggtcga gcggatcgca cgggtggccc
     4621 aatcctcggg catgactgcg ctcgggagca acgacgccgt cgtggagcgt ccgttcgggc
     4681 tcgacggtac gctcgcggag ttcgggcgta cgacgcgcga tcgccgaacc tcgcagcgca
     4741 atcggatgct ggcgggggtc tcgctcttgg cgctgggcgc ggtgggcgga ggttatgtgt
     4801 ggttcaatcg tgggctcgcg cgcttggaat cgcacgtgag cggggcctct tctgcgcccg
     4861 cgctcgagac ccacgcgaac tcgggtcgcg tcgttctcac cgcgcctgtt ccgaccgcag
     4921 cacctccggc ggctgctgcc ccggcgccaa cggcttccgc gccgccagtc gaagcggtga
     4981 gctcgacgcc ggcgccgctt ccgccaccgg ccgcgagcgt cgagggtccg cgcgcgtcgg
     5041 gggcggccaa gcgtgtttcg gagtcgagcc cggcgaagcc caaaacggac aagggcgctg
     5101 cgccgacggg tagctcgaaa gcaccgaaca acaacagccc ctggggcggg cgcctctaag
     5161 agcccgcggt ttccgccggc tctgcgcacg cggaaaaggc cgccccgccg cgagtcttcc
     5221 ctttgccggt gcaggcgagt atcaacgggg ttcgtgaaca ggcttttcct cacttgcgca
     5281 gccctctcga gcgttctggg ggtgaccctg gcccacgccg aggcagaccg tggtgcagcc
     5341 gtggtcttgt tcgatcaggc tcaggcgctg atcgagtccg gtaagttctc cgaagcttgc
     5401 ccgaagttcg cggagagtta tcggctcgac cctcagctgg gggtgctgtt gcacttggcc
     5461 gagtgttacg agcgtaccgg tcggttcgcg agcgcttggg tggcgttccg tgacgcgata
     5521 gagctcgcgg agcggcgttc cgacgggcgc gccgcggccg ccagggagca cgcgtcggcg
     5581 ctcgagccca agctctcgca cctctctgtg gtggtcccga gcgcagcctt ggtggacggc
     5641 ctcaaggtgc aacgcgacgg tatcgagttg agtccggcgc tttggggatc cccgctgccg
     5701 gtcgatccgg gggagcatgt catcgaggtc tctgctcccg gtaaacagag ctttcgaagc
     5761 accgtgagcg tgaaggatcc gggcgaggtg gcgagcgtga atgtgccggt tctttcggat
     5821 ctggcctcca agaacgaggc gccaacggcg ctcgacgtga acgtcgcttc tccgccacgg
     5881 agcagcttct tgtccccacc aacccggact tggctcggca tcggagccct cggtttggga
     5941 gcgacgtccg ctatcgttgg catcgtgttt cacgcgcaac gcgcgagcaa gatagacgac
     6001 cgcgacgcga tctgcccgag cagtatgaat tgcactcgcg acgaggcgaa ccgcatcgat
     6061 cagctcaccg atgaggctcg gagcgcggcg acgatcggga ccgtggggct cgcaatcggc
     6121 ggtgctttca tcgcgaccgg tgcggtgctc ttgctcacgg caccgcgcga cggcggggca
     6181 ccgcgcgagg ctgcgattgg aagaggattg accctgagcc ccgtgttcag tccggctttg
     6241 gggcacgcgg ggagtgcgcc ccgcgcaacc ctgcagggat tagtgatgcg gggaagcttc
     6301 tgaatcgaga cgtcgctcgg ttcgcgctcg catggagcgc gactcaggct catctgcagc
     6361 agcggaatgg gagcggaacc ccatcgagga ccgcgaccgg taagccgtcg ttgttcgcgt
     6421 tacaggcgcc gacgttccac gttacgtagg tgtcatcggc ggcatgatcg ctgtacatgc
     6481 cccgagcttc gcccgaataa ggattgaagt cggcgggcac ggtgctgccg gcgacgccgc
     6541 gtgacgcgca cacttgctgc atctccattt gtgagcacaa gtgatcgccc tgcgccgagc
     6601 aagcaccgat cgcgtcccaa ggtgccgccg gggcgtgcag cgcgccgctt tcgacgcaga
     6661 gatgcggtcc cgcttgatag tagttcttgg ggcagctcaa gccgtctagg cgcgtgcgga
     6721 tagagtcgag ctctttagcc aggtcgtcgg tcgcggcgct cgcgagatct gcgaccttgg
     6781 cgcgctgcgc ttcgagcgca tagggaacca ctccagtctt cgtgcgaccc atcgactcgg
     6841 aatcgacgat cgcttcgagc cagacgtcgg ggctgttttg aaaggccgcg gtgcacttcg
     6901 cattgagcga aacatcgaac tgtcccccga ttggttcgat ctgtgcggtg gtctcgcaaa
     6961 gggggctcgc gtcgtctgaa tcggtcggca gaagccgcag gacgatttcg tgcttgccgc
     7021 tcaacggctg cccagagtcg tcgaacaggc tgccggaata ggtgagtggc tgcttgagcg
     7081 gcacacccgc ggcgctggat cgaccggcca cgaaagcaac gccagcggcc atcacgagca
     7141 gcagcgcgag gccgctcgca cggcgcagtc gcccacgctt cgaggccgat gtgggttcgg
     7201 ggcagggggg ctgattagcg tcatgcatgt tgaagctcac gagtccgatt aggggtgcaa
     7261 agcaccgcgc agggtgaacc actggccgcc caccttggcg tgcagtgcct tgtccccgga
     7321 gatgccgtga tgaaggagac ggtagggctg acccgaggtg accggggccg ggctttgcca
     7381 actgagatgt cctgagctcc gcatgttcga gcagactccg ttttggcaga tcgggcggtc
     7441 accctcgcac tccgctcgag gtaaccactc gccctcctcg caatgttcag gagtattgcc
     7501 gttgcaacga gctgcaccag tttcgcaact ggcccctccc gctccggcgg agccgccttg
     7561 ggcgctcgag tcacccccgc tcgcgttcgt gttcgtgttc gtgctcgtgc ggccgccctg
     7621 agaacgacca ccttccgcag tggccccgcc gctcgccgtc tcgtcgatga tgctgacctt
     7681 gcgatcggac acgcaagccg agcccagaac cgggaagcct gcgagcagga ggaagagcga
     7741 tcggaaagcc atggggatcg agagcggctg cgagttcaaa gagagggtgt tcctgggttt
     7801 taccgaaggt cgtcccgttc gcgcaattct ctcgtttcgg cccttcaata aaccctcatt
     7861 tcgcggcgag ccggcgtcgg gccagcggac gcgatcgagt tcgaggcgca atctgagcgg
     7921 ctctgctgga actacgacac gattgtcagg ggggcgctcg agaggcaggg cgagaaagag
     7981 ggcgcgcgaa caccagcttg cgatgggcgc ggctcttgct cagcagcggc ccaagcctcg
     8041 ctaacgcttg cgcagccttg ctaatgccgg ccgcgtgcct gttcgcgggg tgacttcggg
     8101 agtgtcgcga ctggcgggcc tccagcgaga agatctgttc gaaacgtgaa gcatgttccc
     8161 gacgaggcgc agcgtgggcg cgtcgccggg cccaggtcag gtggagtcag catgtgggac
     8221 cgagttttcc gcagcactaa caacggctgg agcttgagcc tcgcggtggg attaatcgcg
     8281 ggttatctcg ggtgttctgc ctcggattcg acctccacgc gcggaggacc gaatgcgagc
     8341 cattcattcg gaggttccaa agggcaagat ccaccggtgg tcctgcctaa tcccgatcct
     8401 cctccagagc aggaacggga agccgcctat cggttgcccg tgctgagcgg ccgttggatc
     8461 ttcgccgcga acccggagag cggctgggtt gcggccatag atagtcggga cgccagtgtt
     8521 gcgctgagcc gagccggtgc gcagccacgc tatctggcgg cgctgccgcc tgcggatggt
     8581 gcctcggcgc gagccttggt gctcgatccc ggcacggccc aggcccgcga gttgaggctc
     8641 acgcaaacgg gtttgaagcc gagtcagccc ctgcagacgc atcggggtgc gaatgcgctg
     8701 agcgtgagtg aagatggggg gtgggcgttg atttggtctg atgctcgtca gctggaagcg
     8761 ctcgatccgg tagacagcct gcaagacgtg accttgctgg ccttcggctc ggcatcggac
     8821 gagccgcccg cgtcgacgcg actcagcgtt gggtatcgcc ccaaacaggt gattttcgag
     8881 cgtggtaagc gccggctgtt cgtggtgtgt gagcgcacga tcagtatcgt ggagctcggg
     8941 gccgagcctt acgtcgatcg cgacgtcagt ttgcccgtgg ctgcgggtca agcgcccact
     9001 gacatctcgt tgactcctga tggcacgctg gcgctctacc gcgtcgaggg tcaaaccgaa
     9061 gttcattggc tccacctgga ggacctgacc catggagtgg tggcctttcg aaatccggtg
     9121 accgacctgg atctgatgca ggatggcttg cgcgccgtcg tcgtggcgcg aggtcgatcc
     9181 attgtccgtc aaacgtcggc ggacccagcg ggtggcgcgt ccaacgccga agtgggtgga
     9241 gcgagcggcg cgagcggtgt ggttgcgctg gtgggaggcg aggccggcgc aagctctgga
     9301 ggcgtgggag gcgagccgat cacgagcggc ggagcgagtg gggagttccc actagaggta
     9361 gag
//
LOCUS       DASTKU010000376         4394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1632242, whole genome shotgun sequence.
ACCESSION   DASTKU010000376 DASTKU010000000
VERSION     DASTKU010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632242"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VFQ61_12905"
     CDS             <1..1071
                     /locus_tag="VFQ61_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955404.1"
                     /translation="TAKRGIMKVSIVGFSLQGKELARIGGVELNLVDASNEPPPSLPG
                     KEPPMRCYDPGDCPNELKGSPACPGTKAVAGAKTWGAACTASADCQKGLVCMSGTCEQ
                     PPKCDSGAECASGECANGVCVVTDPGEPTKTIGPPKYNWIGLHLALDSMFVRDAVGVC
                     GNTTDDAGDYSCFEGGNEYKGQPNRASAGNVKSGFRFGTIRALLSYERWFGRLSAGAR
                     LGFAIRGAPKGFMPVHAELRLLYSMRPDPLSKRIRPYAGLFAGLMEVQASSRATVIDC
                     IEESLVARQACIEQSDSRTLTTTQAAIKRFDAFRDGSSFGFGPTLGLYTALANDSALN
                     FNLNLVLPNMAFQLSAGYLMGL"
     gene            complement(1207..2154)
                     /locus_tag="VFQ61_12910"
     CDS             complement(1207..2154)
                     /locus_tag="VFQ61_12910"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET9955405.1"
                     /translation="MTRLFRSRKLSSILDAVGNTPLLRLERVARSAPSVEVYVKLEFA
                     NPGGSVKDRPALRMMLDAIADGRLTPDKTLIDSTSGNTGVAYSLIGAALGHKVQLVMP
                     RNVTKARKDITQAFGTELIFSDPMEGSDGAIRLVRKIVSENPERYFYPDQYSNPSNPL
                     AHYLGTGAEILDALGDRITHFVAGLGTSGTMMGTTRRLHEHSRPVHCVAVEPAEALHG
                     LEGLKHMASSIVPPIFDRTTPDEILPVNTEDGWNMADRVAAEEGLHVGHSTGANIFGA
                     VKIAERLHREQGGGCVVTIACDRGDRYFAPLKWERRYTW"
     gene            complement(2151..2432)
                     /locus_tag="VFQ61_12915"
     CDS             complement(2151..2432)
                     /locus_tag="VFQ61_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955406.1"
                     /translation="MADPRYDRQCRLREVGELGQARIESSAARVPPGPSAAPELAFLA
                     RAGVERAAVGTGIEREFSHAVHFRFSAPAALARGAEGALLHLLHALNSE"
     gene            complement(2445..2717)
                     /locus_tag="VFQ61_12920"
     CDS             complement(2445..2717)
                     /locus_tag="VFQ61_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET9955407.1"
                     /translation="MATVRIPTPLRTLTAGEEQVQATGDTVRAVIETLEQKHPGIRDR
                     LLDEKGVRRFVNIYVGDEDIRFLDGLDTQLKASDEISIVPAIAGGR"
     gene            complement(2727..3512)
                     /locus_tag="VFQ61_12925"
     CDS             complement(2727..3512)
                     /locus_tag="VFQ61_12925"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HET9955408.1"
                     /translation="MTPASSSRDSQQRVRSSHGALTGRRVLVVGVGGLGCAALVGLRD
                     SGATFVLADDDRVDLSNLHRQLLFTERDVGRDKLDAARDALLTYGVRAQDVELVRSRF
                     LPENARQLARSVDLVLEGADNFATKFLAADACFLERKPVVHGSAIRFEATAWCVAAAA
                     RPCYRCLFEDVPASGERQLSCNEAGVLGPVVGLCGAFMAELALRYFTGLDAQHDCIHT
                     LDGRRNRVRSIRVAARAGCALCGDQPSIDEIIENRYTAPSCAA"
     gene            complement(3509..>4394)
                     /gene="cysK"
                     /locus_tag="VFQ61_12930"
     CDS             complement(3509..>4394)
                     /gene="cysK"
                     /locus_tag="VFQ61_12930"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET9955409.1"
                     /translation="LVEVRRLERDKPRARVFGKLEAMNPGGSVKDRICLAMIESAEQE
                     GRLLPGGVVVEPTSGNTGIGLALVCARRGYRCVLTMPESMSLERRQLLESFGAELVLT
                     PEAEQMDGAIRKAREIAQQTPKSFMPQQFENGANPEVHARLTAEEVLHAMGNEPIHAF
                     VAAVGTGGTVSGVGRVLKQRVPNCRVIAVEPETCATISRGERGPSKIQGIAAGFVPKN
                     YDGSVVDEVRLVSDERAYQTKRALSRNEGLLVGISAGANVAIALDVARELGPHKNVVT
                     VLCDTGERYFSLDEYFAK"
BASE COUNT          782 a         1438 c         1381 g          793 t
ORIGIN      
        1 accgccaaac gcgggatcat gaaggtcagc atcgtcggct tctcgctgca gggcaaggag
       61 ctcgcgcgaa ttggaggcgt cgagctcaac ttggtagatg ccagcaacga gccgccacca
      121 tcattgccgg gcaaagagcc gccgatgcgt tgctatgacc cgggtgactg cccgaacgaa
      181 ctcaagggtt cgcccgcgtg cccgggaacc aaagccgtag cgggcgccaa gacctgggga
      241 gccgcttgta ccgcgagcgc ggattgtcag aagggtctgg tctgtatgtc gggcacctgc
      301 gagcagccgc ccaaatgcga ctctggcgcg gagtgcgcat ccggagaatg cgcgaacggc
      361 gtttgcgtgg tgaccgatcc gggtgagccg accaagacca ttggtcctcc caaatacaac
      421 tggatcggtc tccatttggc gctcgattcg atgttcgtgc gagacgcggt gggggtgtgc
      481 ggcaatacga ccgatgacgc cggtgactat tcctgtttcg aaggcggcaa cgagtacaag
      541 ggccagccga atcgcgccag cgccggtaac gtcaagtccg gcttccgctt cggcaccatt
      601 cgcgcgctgt tatcttacga gcgctggttt ggtcggctgt ccgcgggggc acgtttgggc
      661 ttcgcgatcc gcggagcacc caaaggtttc atgcccgtgc atgccgaact gcgtttgttg
      721 tactcgatgc gtcccgatcc gttgagcaag cgcattcgcc cgtatgcggg cctattcgcg
      781 gggttgatgg aggtgcaggc ttcgagccga gccaccgtga tcgattgcat cgaggagtcg
      841 ctcgtcgcgc ggcaggcctg catcgaacag tccgattctc gcaccctgac gaccacccag
      901 gcagcgatca agcgcttcga cgcattcaga gatggaagca gctttggctt cgggccaaca
      961 ctggggctct atacggcgct cgccaacgat tcagccctga acttcaatct gaatctggtc
     1021 ctgcccaaca tggccttcca actctccgct ggttacctga tgggtctatg acgcgccgcg
     1081 atcgcggcga ctgacacctc accgagagag ccgcctcgga gcggctcgtc tactgggcag
     1141 cgcccgaggc ggcggctcat cgcgcgcagg cagcagcgag ttcgggttgg agcggtctcg
     1201 gggcgctcac caggtgtaac ggcgctccca tttgagcggc gcaaaatagc gatccccgcg
     1261 atcgcacgcg atcgtcacga cgcagccgcc gccctgttca cggtgcaggc gctccgcgat
     1321 tttcacggcc ccgaagatgt tcgcgcccgt ggagtgtccc acgtgcaggc cttcctccgc
     1381 ggccacgcga tcggccatgt tccagccgtc ttccgtgttc acgggcaaaa tttcgtctgg
     1441 cgtcgtgcga tcgaatattg gcggcacgat cgagcttgcc atgtgcttga gaccttcgag
     1501 gccgtgcagt gcctcggctg gttctaccgc cacacagtgc acggggcgcg agtgctcgtg
     1561 gagccgtcgg gtggtgccca tcatcgtgcc gctcgtgccc aagccggcca cgaagtgcgt
     1621 gatccgatcc cccaacgcat ccaaaatctc cgctcccgta cccaagtaat gggccagagg
     1681 attggacgga ttactgtact gatcgggata gaaatatcgc tccgggtttt cggagacgat
     1741 cttgcgaacc agtcggatcg ccccgtcgga gccttccatc ggatcgctga agatcaactc
     1801 ggtgccgaag gcctgcgtga tgtccttgcg ggccttggtc acgttgcgcg gcatcacgag
     1861 ctgcaccttg tgcccgagcg ccgcgccgat cagagagtac gcgactccgg tgttgccact
     1921 cgtggagtcg atcagggtct tgtccggggt gaggcgaccg tctgcgatcg cgtcgagcat
     1981 catgcgcaat gccgggcgat ccttcaccga accgcctgga ttcgcgaact ccagcttgac
     2041 gtagacctcc acgctcggcg ccgagcgggc cacccgctcc aagcgaagca gcggcgtatt
     2101 gccgacggcg tcgaggatcg aagagagctt acgggatctg aaaaggcgag tcattcggag
     2161 ttcaaggcgt gcaagaggtg gagcagagcg ccctcggcac cgcgcgccaa cgccgcgggt
     2221 gccgagaatc gaaagtgtac ggcgtgcgaa aactctcgct cgatgccggt gccaacggcc
     2281 gcacgctcca ccccggcgcg agccaggaaa gcgagctcgg gagcggcgct cggcccaggc
     2341 ggaacgcgcg cggcggagct ctcgatacgc gcctggccca gctcgccgac ttcccgcagc
     2401 cggcactgcc gatcgtagcg aggatcagcc atacgagact cagttcagcg gccgcccgcg
     2461 atggcaggaa cgatgctgat ttcgtcgctt gctttcagct gagtgtcgag accgtccaga
     2521 aaccggatgt cctcgtcgcc cacgtagata tttacgaagc gccggacacc cttttcatcc
     2581 aacagacgat cccgaattcc ggggtgcttc tgctcgagcg tttcgatcac ggcgcgcacg
     2641 gtgtcaccgg tcgcttgaac ttgctcttca ccagcggtca gagtgcggag gggggtcgga
     2701 atacggacgg tggccatggg atctccttaa gctgcacagc tcggcgccgt gtagcggttt
     2761 tctatgattt cgtcgatgct cggctggtcg ccgcagagcg cgcagcccgc tcgcgccgca
     2821 acgcggatcg aacgcacgcg gtttcgccga ccatcgagcg tgtgaatgca atcgtgctgg
     2881 gcgtctagcc cggtaaagta acgaagcgcg agctcggcca tgaaggcacc gcacaggccc
     2941 acgaccgggc cgagcacgcc ggcctcgttg cacgacaact ggcgttcccc acttgccggc
     3001 acgtcttcga acaggcagcg gtaacaaggt cgcgccgccg cggcgacaca ccaagccgtg
     3061 gcttcgaagc ggatagcaga gccgtgtacg accggcttgc gctcgaggaa gcacgcgtcc
     3121 gcggcgagga acttggtcgc gaaattgtct gccccttcta ggacgagatc gacgctacgt
     3181 gcgagctgac gcgcgttttc cggcagaaag cgcgagcgca cgagctccac gtcttgcgcg
     3241 cgtacgccgt aggtgagcag ggcatcgcga gcggcatcga gcttgtcgcg gccaacgtcc
     3301 cgctccgtga acagcagctg gcggtgcaga ttcgacagat ccacgcgatc gtcgtccgcg
     3361 agcacgaagg ttgccccgct atcgcgcagg cccaccaacg ccgcacatcc caagccgcca
     3421 acaccgacca cgagcacacg tcgccccgtg agcgcgccgt ggctggagcg aacccgttgt
     3481 tgactgtctc ggctcgaaga ggcgggggtc acttggcgaa gtactcgtcg aggctaaagt
     3541 agcgttcacc ggtgtcgcac agcacggtga ccacgttctt gtgaggccca agctctcgcg
     3601 ccacgtccaa cgcgatcgcc acgttggccc cggcgctgat cccgaccagc aagccttcgt
     3661 tgcgcgacaa cgcgcgcttg gtctgatagg cgcgctcgtc gctcacgagc cgcacctcgt
     3721 ccaccacgct cccgtcgtag ttcttgggta cgaagccagc cgcgatgccc tggattttgc
     3781 tcggcccgcg ttccccgcgg gaaatggtgg cacacgtctc cggttcgact gcgatcacgc
     3841 ggcagtttgg cacgcgttgc ttcaagactc ggcccacccc gctgacggta ccgccggtcc
     3901 ccaccgccgc gacgaaggcg tgaatcggct cattgcccat ggcgtgcaga acttcctcgg
     3961 ccgtgagccg cgcgtgcacc tcggggtttg ccccgttctc gaattgctgc ggcatgaaag
     4021 acttgggagt ctgctgcgcg atctcgcgag ctttgcggat cgcgccgtcc atctgctctg
     4081 cttccggcgt caacaccagc tctgcgccga aagattcgag cagttggcga cgctccaggc
     4141 tcatgctctc cggcatcgtc agcacgcagc gatagccccg ccgagcacag accagcgcaa
     4201 gcccaatgcc ggtatttcca ctggttggct ccaccaccac gccgccgggc agtaaccgcc
     4261 cttcttgctc ggcgctctcg atcatcgcca ggcaaatgcg gtctttgacc gagccaccag
     4321 ggttcatggc ttccagcttg ccgaagacac gcgcgcgcgg cttgtcccgt tcgagacgtc
     4381 gcacttcgac cagc
//
LOCUS       DASTKU010000377         6121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1186012, whole genome shotgun sequence.
ACCESSION   DASTKU010000377 DASTKU010000000
VERSION     DASTKU010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186012"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFQ61_12935"
     CDS             <1..479
                     /locus_tag="VFQ61_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955410.1"
                     /translation="GLSGLAGNLAVPLLGLSLAGALVLVSVDRRRIPRKLRDALGLEG
                     VGPLVPLRLPFFQVLYWGTWAAHGYLLAIALGAPPREALSTAGFSPLANVLGFAALLA
                     PAGVGVREAVLMAGLGPVLGAPGAIAAAVLSRVVSLAVDVGTCLLMRAARNRSAAP"
     gene            667..2235
                     /gene="acpA"
                     /locus_tag="VFQ61_12940"
     CDS             667..2235
                     /gene="acpA"
                     /locus_tag="VFQ61_12940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HET9955411.1"
                     /translation="MGKNQVFWSSLRRRGGLGLFTLGVVLTACEEDRAPQGPITRLPA
                     SVTHHRADQPVSPLGTPCPPKAVTGQPLPPINELNPAGLSKLKHFVIIYMENHSFDNM
                     YGQFPGADGIVNSNCEATSALQRDARNLPYSVLPNPSVANWPFPALPNRAFPIAPRVP
                     PSVATIDLVHRFYTQQAQINFGGMNKFVAYSDARSLTMGFYDTMLLPVPAIAANYTVC
                     DRFFHSAFGGSFLNHMWLVGARSPEFPGAPPNIVEPGLPIEYDPALPFPINADGSFTG
                     ADYEVTPDGFAVNTLYSVNTPHPLDPALVPQRLPAQTYATIGDRLSDAGVSWAWYSGG
                     WDDAIAYSTGAAPAVLDEFQFHHQPFAYFANYGDGTPGRAAHLKDETEFLRAVKQGTL
                     PEVSFVKPVGIDNEHPGYTDVLRGENYLADLIQSIMMGPNWPDTAIIIAYDENGGFAD
                     HVPPPVIDRWGPGTRVPAIVISPYARRGFIDHTQYETVSILTTLERRYGLPPLTSRDA
                     NANDLSPAFDFDTE"
     gene            complement(2297..3430)
                     /locus_tag="VFQ61_12945"
     CDS             complement(2297..3430)
                     /locus_tag="VFQ61_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET9955412.1"
                     /translation="MGKVVNMAARRGSGALEKALRALAIAGAAWSCSVNEKQRCYDEC
                     PDSGGRAGARGPSPSVASGGKTGSSTTGTSGGAGGGASGGDTATTAGTSSSSSNAGAG
                     GTAGAPATGASGGQRTSGGTAGAASGGLTSTLDGGGAGGEEPTGSGGSGTGGRAELLR
                     DCRLLLHMDEAKWNELTGSVRDASGSGNHGTAKGGATTGAMGKFDRAGAFDGNGYVDL
                     GDNERLEPGDAFTLALWVYPMGMTGDSAGLVAKRWAFGDRSAFALFLMDQNLVYVDVD
                     GEGERFTSSQALTAESWHHVALVFDGGAPAEQRVRLYLDGQLDSTHAEASSSIPDYDS
                     PFEVGRLRDGGGNLIGQLDEVGFWQRALTTQEITQLSSATEGL"
     gene            complement(3637..3786)
                     /locus_tag="VFQ61_12950"
     CDS             complement(3637..3786)
                     /locus_tag="VFQ61_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955413.1"
                     /translation="MKRVLLITGAGRGISARCGNRAIGGKERQLDSDEASFTTGALVD
                     VSGRR"
     gene            complement(3783..4715)
                     /locus_tag="VFQ61_12955"
     CDS             complement(3783..4715)
                     /locus_tag="VFQ61_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9955414.1"
                     /translation="MRGSSEVQVRAAVEGDYPEYCRLFPELGVDDPVPPRELWVNLLM
                     PMTLVAVPARSTGVPTNSVPSANSEPGPDCILGYCFFGVLTDCGYIRNLVVAPEVRRR
                     GVGRALMQAMASYLLAHGKSSWCLNVKSDNQAAIKLYGACGMRIRYRSWALKLPWSAL
                     SSLRDAELVHGNSTDPHAAIVVRVSPLPLEHEAEYEARLDLPRGLIAEGRASGRLLLE
                     ARGWSDDPKLDTSGGVALGLAILNPKMPGAFPFRVLHACAALPLLERIRSQVPESAML
                     NLVVEEDRSTCERLLSAGAQLKFDMVHLAGALAR"
     gene            complement(4715..5287)
                     /locus_tag="VFQ61_12960"
     CDS             complement(4715..5287)
                     /locus_tag="VFQ61_12960"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET9955415.1"
                     /translation="MDGIQGLENLDLRCSGCGECCKRLRVTLTHRDLARLRSFTSEPA
                     SRWVDWLAPDLVDMQGEPESFVELREGRRLMVLAHAQGACWFLSADQRCSVYAARPRD
                     CRVFPYDWQREREGRVVRLALLPEPNCVLQTGPRSSLPLELAEDDERWAELRQYQDLV
                     ARWNRLASRRRRFGHRLGSAQEFIEYLEVK"
     gene            complement(5501..>6121)
                     /locus_tag="VFQ61_12965"
     CDS             complement(5501..>6121)
                     /locus_tag="VFQ61_12965"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955416.1"
                     /translation="LQADLRGVRSAKWITVISLTERLGYPQPVGNDSFSDASLGFGFG
                     AWGARRGIVRRAERRRDHSSGGSFACAVVAGSARCWGNNDSGQLGNDSVVRSSIPVPV
                     RGLESIVSAVSAGAYHSCAVVDGAAWCWGDNQYGQLGDGSTLDSLVPVRVAGLTSGVS
                     AIASGTYHTCAIVKGRVRCWGHNDSGQLGLGDNQDSLVPMVVTWPL"
BASE COUNT         1163 a         2131 c         1726 g         1101 t
ORIGIN      
        1 gtggcttgtc tggcttggca ggcaacctcg cggtgccgct gctgggcttg tccctggccg
       61 gcgcgctcgt gctggtgagc gtcgatcgcc ggcggatccc acgcaagctc cgagacgctt
      121 taggcttgga gggagtgggc ccgctcgttc cgttacgttt gcccttcttc caggtgttgt
      181 actggggcac ttgggccgcg cacggctacc tgcttgcgat cgcgctcggc gcaccaccgc
      241 gtgaagcgct atccacggct gggttctcgc cactcgcgaa cgtcttggga ttcgcggcgc
      301 tgctcgcgcc agctggagtt ggcgtgcgcg aagccgtgtt gatggcggga ctcgggccgg
      361 tgttgggcgc gcccggcgcg atcgcggccg ctgtcctctc ccgagtcgtt tcgttggcgg
      421 tggatgtggg cacttgcctg ctgatgcggg cggcgcgcaa ccgatctgca gccccatgat
      481 ccacgctcga aacggttcaa cactgtaaag atctgcgtat ccggatcagc accgcctccg
      541 cggttccctg agcgcggtaa tctccgctcc gcgagccgtc atatccaggt agcgctccga
      601 tcccggtagc tgtcatatcg aggtagccgg ttggagctcg ccgatctaaa cctcaggaga
      661 gttctgatgg gaaaaaacca ggtgttctgg agctcgctcc ggcggcgggg tggtctcggg
      721 ttgttcactc tcggggttgt tctcacggct tgtgaggagg accgagcgcc gcaagggccg
      781 atcacgcggc tgccagccag cgttacgcat catcgcgccg accagcccgt gagtccgctc
      841 ggaacgccgt gcccaccgaa agcggtcacc gggcagcctc tgccacctat caacgaactt
      901 aaccccgcgg gcctctcgaa gctcaagcac ttcgtgatta tctacatgga gaatcacagc
      961 ttcgacaaca tgtatgggca attccccggg gcagacggca tcgtgaactc gaactgtgag
     1021 gccacgagcg cactacagcg ggacgcccgg aacctgcctt actcggtgtt accgaatccc
     1081 agcgtcgcaa actggccttt ccccgctctg cccaatcgcg ctttcccaat tgcgccgcgc
     1141 gtaccgccga gcgtcgcaac tatcgatctg gtgcatcgct tctataccca acaggcccaa
     1201 atcaattttg gcggcatgaa caagttcgtc gcatacagcg acgctcgttc cctcactatg
     1261 ggcttctacg acaccatgtt attgccggtg ccggcgatcg ccgccaacta cacggtttgc
     1321 gaccgcttct tccactctgc cttcggcggc tcctttctca atcatatgtg gttggtcggc
     1381 gcacgcagcc ccgagtttcc tggggctccg cccaacatcg tcgaaccggg gcttccaatc
     1441 gaatacgatc cggcgctgcc gtttcccatc aacgccgacg gcagcttcac gggggcagac
     1501 tacgaggtga cgccggacgg cttcgcggtg aacaccttat acagcgtgaa caccccgcat
     1561 ccgctcgatc ccgccctcgt gccgcagcga ttgcctgcgc aaacctacgc cacgatcgga
     1621 gaccggctct ccgatgccgg tgtgagttgg gcttggtact cgggaggttg ggacgatgca
     1681 attgcttact ccaccggagc ggcgccggcc gtgctggacg agttccaatt tcatcaccaa
     1741 cccttcgcct atttcgccaa ctacggtgac ggaacgcccg ggcgcgctgc gcacttgaaa
     1801 gacgaaacgg agtttctacg agccgtcaag cagggtactt tgcccgaggt ttcgttcgtg
     1861 aagccggtcg ggatcgacaa cgaacatccc ggttacacgg acgtgctgcg cggcgagaac
     1921 tacctggcag atctgattca atcgatcatg atggggccga actggccaga cacggcgatc
     1981 atcatcgctt atgacgaaaa cggcggattc gcagatcatg ttccgccccc cgtcatcgac
     2041 cgctggggcc ctggcacgcg cgtgccggca atcgtgatct ctccgtacgc acgtcgcggg
     2101 ttcatcgatc atacgcagta cgaaaccgta tccatcctca cgactctaga acgtcgttat
     2161 ggtctgccgc cgctcacatc acgcgacgcc aacgcgaacg acctcagccc cgcattcgat
     2221 ttcgacaccg aatagcaggg ccgctcgagc gcactcagcg cccgagccag ccccacgctc
     2281 acgtgagagc gctgcgctat agaccttccg tggccgagct cagctgcgtg atctcctgcg
     2341 tcgtgagcgc gcgttgccaa aacccgactt cgtcgagctg accaatcagg ttacccccgc
     2401 cgtctcgcag tcggcccact tcgaaaggcg aatcgtagtc gggaatggat gagctcgctt
     2461 cggcgtgagt cgaatccaat tgcccgtcta gatagagccg cacgcgctgc tcggcgggcg
     2521 cgccgccatc gaacaccagc gcgacgtgat gccagctctc cgccgtcaaa gcttggctcg
     2581 atgtgaagcg ctcgccctca ccatcgacat ccacgtaaac caagttctga tccatgagga
     2641 agagcgcgaa cgcgctgcgg tccccgaatg cccagcgctt ggcaacgagc cccgcgctgt
     2701 cccccgtcat tcccatcgga tagacccaga gcgctagcgt gaaggcgtcc ccgggctcca
     2761 gccgctcgtt gtcgcccaag tcgacgtaac cgtttccatc gaaagctcct gcgcgatcga
     2821 atttgcccat cgcgccggtg gtggcgccac ctttggcggt gccatggttt ccggagccgg
     2881 atgcatctct gaccgaaccg gtgagctcat tccacttcgc ttcgtccatg tggaggagca
     2941 gccggcaatc ccgcagtaac tctgctcttc cgcccgtgcc cgagcctccc gatcccgtag
     3001 gctcctcacc acctgcgccg ccgccgtcga gcgtcgatgt caggccaccg ctcgccgcac
     3061 ccgctgtgcc tccgctggtg cgctgcccgc cgcttgcgcc agtcgccggg gcccccgccg
     3121 tgccgccggc gccggcgttg gaactcgaac tggacgtgcc ggccgttgtt gcggtgtctc
     3181 cccccgatgc accgcccccg gccccaccgg atgtgccggt cgtcgaagat cccgtctttc
     3241 ctcccgatgc cacgctcggc gaaggaccgc gagccccagc tcgaccgccg gaatccgggc
     3301 actcgtcgta gcatcgctgt ttctcgttga ccgaacagct ccacgccgcc cccgcgatag
     3361 ccagcgccct cagcgccttt tcgagtgcgc cgctaccgcg ccgggcagcc atatttacca
     3421 cttttcccat gacaccactc tccgtgcggg ggacagtcta aaacaacttt ccctcgaaac
     3481 aaccgctcga cccgcaatta aacgtcggca cccaagacga ggctgaggtc gcgaggaact
     3541 gcccgcgtcg agcgaggagg cgcgaaatcc ggattggagg agcaatggag tcatagcatg
     3601 ggggtgaggc cgccttgcgt cgcatcgaga cggcggtcag cgccgaccag aaacatcgac
     3661 caacgcgccc gtcgtgaacg aagcttcgtc ggagtcgagt tgcctctctt tgccgccaat
     3721 cgcgcggttg ccgcaccgcg cgctgattcc tcgccccgct cccgtaatca acaacacccg
     3781 cttcatcgcg ctagcgcccc ggccagatgc accatatcga acttcagttg cgctcctgcg
     3841 gatagcagcc gctcacaggt ggagcgatct tcctcgacca ccaggttcaa cattgcgctt
     3901 tccggcacct gacttcggat ccgctccagg agcggcaacg ccgcgcaagc gtgcagaacc
     3961 cgaaacggga atgcgccagg catcttcggg ttcaaaatgg ccagccccag cgccactccg
     4021 ccgctcgtat cgagcttcgg gtcgtccgac caccctcgcg cctcgagcag caaccgccct
     4081 gacgcgcgcc cctcggcgat gagacctcgg ggtaaatcca gacgggcctc gtactcggct
     4141 tcgtgctcga gtggcaacgg gctcacacgc acgacaatcg ccgcgtgcgg gtcggtggag
     4201 ttgccgtgaa ccaactccgc atcgcgcagg gaagaaagtg cactccaggg caacttcaag
     4261 gcccaggacc gataccgaat ccgcatgcca caggctccat agagcttgat ggccgcctgg
     4321 ttgtcggact tcacattcag acaccaactc gacttaccgt gagccaggag ataactcgcc
     4381 atggcttgca tcaatgcacg gcccacacca cgacgccgca cctcgggagc aacgaccagg
     4441 ttgcggatgt agccgcaatc cgtgagcact ccgaagaagc agtatcccaa gatgcagtcc
     4501 ggtcctggct ctgagttcgc ggaagggact gagttggtag gtaccccagt acttcgagcc
     4561 ggcaccgcga ctaaagtcat tggcatcagc aaattcaccc agagctcacg aggcggcaca
     4621 ggatcgtcca ccccgagctc gggaaaaagc cgacaatact ctggatagtc gccttcgacg
     4681 gccgctcgga cctgtacctc actcgaaccc cgcatcactt tacctccaaa tactcgatga
     4741 attcctgagc cgagcccaag cgatgaccga aacgcctgcg tcgcgacgcg agtcgattcc
     4801 agcgagcgac caagtcctgg tattgacgaa gctccgccca acgctcgtca tcctccgcca
     4861 gttccagagg cagcgaggaa cgagggccag tctgcaagac acagttcggt tccgggagca
     4921 acgccagccg cactacgcgc ccctcgcgct cgcgctgcca atcataggga aacacgcgac
     4981 agtcccgcgg acgcgccgcg tacaccgagc aacgctgatc cgctgagaga aaccaacagg
     5041 ccccctgcgc gtgggccaaa accatcaagc gccgaccttc cctgagctcc acgaagctct
     5101 cgggctctcc ctgcatatcc acgagatccg gagccaacca atcgacccag cgtgacgcag
     5161 gctcggacgt gaaagaccgc aggcgcgcga gatctcgatg cgtaagagtc acccgcagcc
     5221 gcttgcagca ttccccgcaa ccgctgcagc gcaggtctag attctcgagc ccttgaatac
     5281 catccaaatc ggcctcgagc gtagcggctc ccctgcggac tgtcgagcag gccgactacc
     5341 acgcgcccgc accggcgaag cattggcttt ttcgtcggct ttacggaatg ggcgcgcgta
     5401 ccggggggtc gctcaacgcc accgcgcctg gttcgccccc gacctggcgt cgtttacccg
     5461 gttcagccgg cttcaagcct cgaacgagct ggtcagtgcg tcagagtggc cacgtcacca
     5521 ccatcggcac caagctgtct tgattgtctc caagacccag ctgcccagaa tcgttgtggc
     5581 cccagcatcg aacgcgccct ttgacaatcg cgcaggtgtg atacgtgcct gacgcaatgg
     5641 cggacactcc gctcgtgagc cccgcaaccc gaacaggaac taggctatcc aaggtggagc
     5701 cgtccccgag ttgaccatat tggttgtctc cccagcacca ggctgcaccg tcgacgacgg
     5761 cacaactatg atatgcgccc gccgataccg cgctcacgat cgattcgagc ccgcgcaccg
     5821 gcaccgggat cgaacttcga accaccgagt cgttacccaa ctgcccagaa tcgttgtttc
     5881 cccaacagcg cgccgaaccg gcaaccaccg cgcaggcgaa cgaccctccc gacgaatgat
     5941 ctcgccgtcg ttcagccctc cgcacgatcc cacgacgagc cccccaagcg ccgaagccaa
     6001 aacccaaact cgcgtccgag aacgaatcat ttccgaccgg ttgaggatat ccaagcctct
     6061 cagtgagcga aatgacggtt atccacttcg cgcttcgcac tccgcgtaaa tcagcctgta
     6121 a
//
LOCUS       DASTKU010000378         4234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1410294, whole genome shotgun sequence.
ACCESSION   DASTKU010000378 DASTKU010000000
VERSION     DASTKU010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410294"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1524
                     /locus_tag="VFQ61_12970"
     CDS             187..1524
                     /locus_tag="VFQ61_12970"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9955417.1"
                     /translation="MWLYPREIFVRFTPDALTLGRASDCGVVLEGGLVSRVHATVRPL
                     GPLFVLRDEGSRNGTYQNGEAVREAPLVNGDIVRIGEWVGCVIELDEAVAASGAYFRS
                     EEGGLLLGPRSQESWQRAARVAESSAPILIEGPTGTGKEVFARAIHARSRRGGSFVAV
                     NCAALPESLVESHLFGHAKGSFTGATQSTEGLIGAAQRGTLLLDEIIDLPTHQQAKLL
                     RVLEESSVVRVGETTPRPIDVRFVAACQEPLWKRIEKGSFRADLLGRLAGFTLKLSPL
                     RERREEIPRLFLQAFAAAGGDAQRIKSSAIEALCLESWPLNVRQLVHVARYAATGMVP
                     GAPITRSSLSEALGEAPPQLERQSESPPDEPAIIVSEAAAEPRSEEPDSAESRLGRRR
                     MLWLRRQEEQLSQLLNALKQSKGNVSMAARSVGLSRSAATRLLEAKAQREGER"
     gene            complement(1543..3033)
                     /locus_tag="VFQ61_12975"
     CDS             complement(1543..3033)
                     /locus_tag="VFQ61_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955418.1"
                     /translation="MNDACSVGYCDTELGCVRQRKTLAGLGCASPLKLDLNNGAGHGR
                     ADTTCGAPRAELVTRGAPGNAAWFEIDLTEFKQPVTADLLVGAAFPVDSVLLKGQCED
                     SFVVEYAHAENWAGEPGAVRLTKELEPAVYRLIVASLAPDQLGPIELAARITEASKSL
                     DPFDLGLAKSCQELIELRSEPGVQVRLDLITEVTPIAACAQKYYRSAPCYSLDLRDRE
                     VASLVQAEVRGGSAAWTPFESAGTCEGDGFFSALLAPARYKLWLSTNHSNEPVALRLT
                     IVDRSCEPYPNDACERAIPILLNDEHRAEITGDASCGTLRVQSIWGSQEPQLFYRLDL
                     SEWAGPVRLRIREKSGSGAKVLVLESSQDSSCSESLHQCEDCVVAPRAYLLAVQGSTV
                     YDFELSASSVPPPSAATCIPNSVMSCAWHSVRECQQYRLNHPRCVAVLESCGLDWPPL
                     KELCQSHPECCDEALATPNAPNFCQDLFIAAGAACPDCLDSGCWLY"
     gene            complement(3417..>4234)
                     /locus_tag="VFQ61_12980"
     CDS             complement(3417..>4234)
                     /locus_tag="VFQ61_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955419.1"
                     /translation="APGLYALELESPPARPAYLRVEFHRPGCSGNVHDTCANAEDIQL
                     TGEQATVRGHTYCGREQYGREHCVGPSPKRFYRLDLRDRKDRVRLRAKIPPRDLGFYA
                     AVTLLRDEGPDCDALLLQCFDSMGNGDGWPLVDSIVEPGVYFLVVEGVELGAAGEFTL
                     EVDVSDWRTRRFSPCYGGDIEGCLYNTNDAADVCWSNPLHESCATMFTSCGLSPETQA
                     CICDSDPVCCAGTDEDAERCAPVFASCNFFCEDVRWADPFIDRSRLFSLLYDP"
BASE COUNT          805 a         1281 c         1399 g          749 t
ORIGIN      
        1 gccaagcatg actggcccat gtgagcaacc ggaacgatcc gggatgacgt tttcgtcggc
       61 aggggctgag ccggagtgat agcctgccgc ggtgtaatgg tgaggtaccc cgctcgcgag
      121 gagagtttcg aaactgcgcc cgctacgggc agtcatgctc cagaccgtcc caggagtctt
      181 ggaatcttgt ggctgtaccc acgggagata ttcgtacggt tcactccgga cgctttgacc
      241 ctcggtcggg cctcggactg cggcgtggtg ctggagggag ggctggtctc gcgcgtgcac
      301 gcgaccgtgc gtccgctcgg acctctgttc gtgctgcgag atgaaggcag tcggaatggg
      361 acttaccaga acggtgaggc cgttcgggag gctccgctcg tcaacggcga catcgttcgg
      421 atcggcgagt gggtcggttg cgtgatcgag ctcgacgagg cggtcgctgc ttccggcgcg
      481 tactttcgca gcgaagaagg agggctgctg ctcgggccga ggtctcaaga gtcttggcag
      541 cgcgctgcgc gagtcgcaga gagttccgca cccattctga tcgaagggcc gactggaacg
      601 ggcaaagagg tctttgcgcg ggcgattcac gcgcgcagtc ggcgcggcgg ctctttcgtc
      661 gcggtaaatt gcgcggcgct tccggagtcg ctcgttgaat cccacttgtt cgggcacgcc
      721 aaggggtctt tcacgggcgc cacgcagtct acggagggac tgattggggc ggcccaacgc
      781 ggcaccctcc tgctggacga gatcatcgat ttgccgacgc atcagcaggc gaagctactt
      841 cgggtcctcg aggagtcctc cgtggtgcgt gtcggtgaaa caacgccgcg gccgattgac
      901 gtgcgcttcg tggcggcgtg tcaggagccg ctctggaagc ggatcgaaaa gggttctttc
      961 cgggccgacc ttttgggtag gctcgcgggc ttcacgctca agctgagccc gctcagggaa
     1021 cgtcgtgaag agatccctcg cttgttccta caggcgttcg cggccgccgg cggggacgca
     1081 cagcgcatta aatcgagtgc catcgaggca ttgtgtctag agtcgtggcc gctcaatgtt
     1141 cgtcagctcg tgcatgtggc gcgctacgcg gcgacgggca tggtgcctgg tgctccgatc
     1201 acgcggagct cgctgagtga agccttgggg gaggccccac cgcagctcga gcggcaaagc
     1261 gagtcaccgc cggacgagcc cgcgatcatc gtaagcgaag ctgcggccga gccgcggagc
     1321 gaagagccgg actccgccga gtcgcgactg gggcgccggc gcatgctctg gctgcgccgg
     1381 caagaagaac aactcagtca actgctgaac gccctgaagc aatccaaggg caatgtatct
     1441 atggccgcgc gtagcgtagg cctgtctcga agcgcagcca cgcgactact cgaggcgaag
     1501 gctcaacgtg agggtgagcg ctagatcagg tttatcggcg cgctagtata accagcatcc
     1561 actgtcgagg cagtcgggac aagcggctcc ggccgcgatg aacaggtcct gacaaaaatt
     1621 gggggcgttc ggcgtagcga gtgcctcgtc gcagcactcc ggatgcgact ggcaaagctc
     1681 tttcagaggc ggccaatcga ggccgcagct ctccaatacg gcgacgcagc gcggatggtt
     1741 cagcctgtat tgctgacatt ctctgaccga gtgccaggcg cagctcatca cggagttcgg
     1801 aatgcaggtc gccgccgaag gcggcggcac gctactggcg ctcagctcga aatcatagac
     1861 agtgctgcct tgaacggcga gcaggtaagc gcggggggcg acgacacaat cctcgcactg
     1921 atgcagtgat tcggaacatg acgagtcttg ggatgattcg agcacgagaa cctttgcgcc
     1981 gctaccggac ttctcgcgga tgcggagcct gaccgggcca gcccattccg aaagatcgag
     2041 tcgatagaag agttgtggct cctgcgaccc ccaaatgctc tggactcgca gggtcccgca
     2101 gctcgcgtct ccggtgattt cggcgcggtg ttcgtcgttc agaaggattg ggattgcgcg
     2161 ctcgcaggcg tcattggggt acggctcaca cgagcggtcc acgatcgtga gtcgaagggc
     2221 aacgggttca ttcgagtggt tggtcgacag ccacagctta tagcgcgccg gtgccagtag
     2281 tgcgctgaag aatccatcgc cttcgcaagt gcccgccgat tcgaacggtg tccacgccgc
     2341 agatccccct cgtacttctg cttgaacgag cgaggcgacc tcgcgatccc taagatccaa
     2401 cgagtagcac ggcgccgatc gatagtattt ctgcgcacaa gccgcgattg gagtgacctc
     2461 ggtaatgaga tccagacgca cctgaacgcc gggctccgac cgaagctcaa tcaactcttg
     2521 gcaggacttc gcgagaccca agtcgaaggg gtccaaggac ttggaggctt cggtgatccg
     2581 cgctgcgagc tcgatcggtc cgagttgatc aggggcgagg ctcgcaacaa tcagcctgta
     2641 aacggccggc tcgagctcct tcgtcaaccg aaccgcaccg ggctcacccg cccaattctc
     2701 cgcgtgggca tactcaacga cgaacgagtc ctcgcattgc cccttcagaa gcaccgagtc
     2761 gacggggaag gcggcgccca cgagaaggtc tgccgttacg ggctgtttga attccgtgag
     2821 atcgatttcg aaccacgccg cgttgcccgg agcaccgcgg gtgacgagct cggctcgagg
     2881 ggcaccgcaa gtcgtgtctg cccgcccgtg tccggcgcca ttgttcaggt cgagttttag
     2941 gggggaggcg caaccgaggc ccgctagggt cttgcgttgg cgcacacagc cgagctctgt
     3001 atcgcagtaa ccaacggaac aggcatcatt caagtcggaa cagtcgggcg ggtgagccgg
     3061 aacgcagcga ccgtcttcgc accggccttc cccgcagggg ccggcttcgt cagcgcaggg
     3121 ctttccgttt tcaacggctc tgagctggca cgaggttccc cactcgctgc actcaccaac
     3181 ggtgcagggg ccgttcaggt tggcgcaact cgacgcgtcg caggtcgctg caggcacggg
     3241 cggcaattcg ggcggcgccg tcgcggcggg cgagggggcg ccctgttcgg cgcgagctcc
     3301 gagcgcgcaa gcatggattg cccgaagcgc gagcagagac gcgtagacgg agacccatcg
     3361 agcaccacga gtgaattgca ttggagcagg ggacgttcgg tgcaaagcgg gctcgctcac
     3421 gggtcataca acaacgagaa gagccgactc cgatcaatga aggggtcggc ccagcgcaca
     3481 tcctcgcaga agaagttgca gctcgcgaac accggagcgc accgctccgc gtcttcgtcc
     3541 gtgcccgcac agcagactgg atccgaatcg cagatgcagg cttgagtctc gggagaaagg
     3601 ccgcaggaag tgaacatcgt cgcgcagctc tcgtgcagtg ggttgctcca acatacgtcg
     3661 gctgcgtcat tggtgttgta gaggcaaccc tcgatgtctc cgccgtagca cggggaaaac
     3721 ctcctcgtcc gccagtcgct gacatccacc tccaaggtga actcgcccgc agcgccgagc
     3781 tcgacgcctt cgaccacgag aaagtacacg ccgggttcga cgatcgagtc gacgaggggc
     3841 cagccgtccc cgttgcccat cgaatcgaag cattggagta gcaacgcatc acaatcggga
     3901 ccctcgtcgc gcagtaaggt tacggccgca tagaacccga gatcgcgagg cgggattttc
     3961 gcccgaaggc gtactcggtc cttccgatca cgcaaatcca gacggtagaa gcgcttcggg
     4021 gagggcccga cgcagtgctc tcggccatac tgctcacggc cgcagtaggt gtgcccgcga
     4081 accgtagctt gttcgcccgt cagctggatg tcctcggcgt tggcgcaagt atcgtgtacg
     4141 ttaccggagc agcccggacg atgaaattcc accctcagat acgcgggtcg agccggcggg
     4201 ctctctagct ccaatgcata cagaccgggc gcca
//
LOCUS       DASTKU010000379         6514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_739612, whole genome shotgun sequence.
ACCESSION   DASTKU010000379 DASTKU010000000
VERSION     DASTKU010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739612"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFQ61_12985"
     CDS             <1..556
                     /locus_tag="VFQ61_12985"
                     /inference="COORDINATES: protein motif:HMM:NF013938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955420.1"
                     /translation="PYFEVRAIAARHANLFKLLPLVEDPDETVRSSVATRVPQSVLRR
                     MVNDPHREVRIRVAQRLAPPSLVLMLRDEDYYVRVWVVRRIAPALLPRFVDDPEREVR
                     LEVAERLSPGALGLLADDQDHSVRRAVARRAPDRLLEQLAHDPVWEVRWEVAQRANKR
                     VCELLKFDPEPEVAFAAIERLKQL"
     gene            complement(734..2476)
                     /gene="nifA"
                     /locus_tag="VFQ61_12990"
     CDS             complement(734..2476)
                     /gene="nifA"
                     /locus_tag="VFQ61_12990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HET9955421.1"
                     /translation="MSTSFDLSPMEPVNVGERTGSTRSISAARRAEVQLYGVYEISKL
                     LASPARLELTLAHVVHLLASFLDMRHGLIALLDEYGAPEIVVGSGWSEATARRYFEHL
                     PERAIGRIVATEMPLVVRSIREDPLWENWDFSESGVGCDVSFVGVPIRDNGRVIGTLT
                     IDREARPEDFFALDEDVRFLTMVANLVGQTIRLQRLVARDRERLMEDRRRLERELEQA
                     VPERKESVLNGIVGDSTALHSVIEKIRVVARSNSTVLLRGESGTGKELFARALHDLSP
                     RKGQAFVKVNCAALPESVLESELFGHEKGAFTGAAAMRKGRFELAHGGTLFLDEIGEI
                     SASFQAKLLRVLQEGEFERVGGTSTLKVDVRLVAATNRNLEEAVANGEFRADLYYRIG
                     VIPIFLPALRDRKSDIPILADEFLRRFNGEHGLNLEFARDGLDVLSSCLFPGNVRELE
                     NCVRRTATLANGRAIRADDFACTKDECLSAALWQHPQRAATMPPGFVPLPITRGPRSG
                     PFANEVRPSSPPHPIGDEETEFGEDSSDERQRLIQAMEAAGWVQAKAARLLGLTPRQI
                     GYALKKYDISIKRF"
     gene            3020..4183
                     /gene="nifV"
                     /locus_tag="VFQ61_12995"
     CDS             3020..4183
                     /gene="nifV"
                     /locus_tag="VFQ61_12995"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR02660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HET9955422.1"
                     /translation="MQRPPIVVNDSTLRDGEQAPGVAFRIDEKVAIARALEAAGVDEL
                     EAGIPAMGAREVEAMQAVQAALEKARAIAWCRMTDADVEAALASGLSRVNLSIPVSDR
                     QMRVKLGVGRRAVLGRIQRTVRYAIDRGLVVHVGGEDASRADLDFVCRAVVAAEEAGA
                     VRFRFADTVGVMDPFLTHAVFQRLCAETDLELEFHGHDDLGLATANTLAAVRGGATHV
                     SVCVLGLGERAGNAPLEEVAAALTAIEGLRTGIDPTRLPALAEQVAVASGRPIPDGKA
                     IVGRSAFTHESGIHVAGLLKDRLTYEALPPERFGRSRRIVLGKHSGRAAIDHALQLLG
                     VAADDRSLGVVLERVREHAERTKAAIGNNELLEFYVAARASSEHRRSSRTRTR"
     gene            5804..>6514
                     /locus_tag="VFQ61_13000"
     CDS             5804..>6514
                     /locus_tag="VFQ61_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9955423.1"
                     /translation="MNRRLLDLVSRVQASTDIPSLLSAVMDCTRDVTGAQVAGVAVLV
                     NQGYQVSQVGAPDYLLESYTQFTVSAADPVHEALTQQHGVIRTDDLFTPSAWHGHPFY
                     QQVEAPSGLDSYMAAEIVDAKGVRGFVGASRRTGQPPFSSADASLLQTACLHISAALT
                     RLGSVIPLQSLTPTEREVVRLVARGFTNQQIGQARGVTAHAVKKTLERLFEKIGVTSR
                     TELIAALGVSTEPSRWGRA"
BASE COUNT         1235 a         2073 c         2052 g         1154 t
ORIGIN      
        1 tccctacttc gaggtgcgcg ccatcgcggc ccgtcatgcg aacttgttca agctgcttcc
       61 cctggtggag gaccccgacg agacggtgcg aagctcggtg gcgacgcgcg tgccgcaaag
      121 cgtactccga cgcatggtca atgatccaca ccgcgaggtt cgtattcggg ttgcgcagcg
      181 cctagcgcca ccatcgctcg ttctgatgct gcgggacgag gactactatg ttcgcgtctg
      241 ggtcgtgcgg cggatagcgc ctgcgctgtt gccacgcttc gtggatgacc cggaacgcga
      301 ggtgcggctc gaggtggcgg aaaggttgag cccgggcgca ctcggcctcc tcgctgacga
      361 tcaggatcac tccgtgcgcc gggcggtggc tcgtcgtgcg ccggatcgcc tgttggagca
      421 gctcgctcac gaccccgttt gggaggtgcg ctgggaggtg gcgcagcgag cgaacaagag
      481 agtatgcgaa ctgctgaagt tcgaccctga gccggaggtc gctttcgcgg cgatagagag
      541 actcaagcag ctctgaactt cgctaccgcg tccgctgccg ctctgagctc cgctgcctct
      601 ctgcgctccg ctgccgctct ggcggcagcg gcagctcgag tcgacggttg ctcgaggctc
      661 gtcctgcttt ccgatgagct ctccgctgct cggtgagatc ccgcgaaagg ggtagccgta
      721 attgcagctg ggctcaaaag cgtttgatcg agatatcgta tttcttcagc gcataaccaa
      781 tttggcgcgg ggtcagcccg agcaagcggg cggctttggc ttggacccag cccgcggctt
      841 ccatcgcttg aatcaggcgc tggcgctcat ccgaggaatc ctcgccgaac tcggtctcct
      901 cgtctccgat cgggtgcgga gggctcgaag gccgaacctc gttcgcgaaa ggccctgagc
      961 gcgggccccg cgtgatgggc agaggcacga acccaggagg catagtcgcc gcgcgttggg
     1021 gatgctgcca gagcgccgcg gagagacact cgtccttggt acacgcgaaa tcgtccgcgc
     1081 ggatcgcacg gccgttcgcc agcgtggccg tgcgacgaac gcagttttcg agctcgcgca
     1141 cattgcccgg gaatagacag gacgaaagta catccaaacc gtcgcgcgcg aactctagat
     1201 tcagcccgtg ttcgccgttg aatcggcgca gaaactcgtc ggccaagatc ggaatatccg
     1261 acttgcgatc tcgcagcgcg ggcaagaaga tcggaatcac accgatgcga tagtataggt
     1321 ccgctcgaaa ctcaccgttt gctacggcct cttccaggtt gcgattggtc gcggccacca
     1381 gcctcacgtc caccttgagc gtgctggtac cgcctacgcg ctcgaactcg ccttcctgca
     1441 acacgcgtag caacttcgct tggaaggagg ccgagatctc accgatctcg tccaagaaca
     1501 gcgtgccgcc gtgcgccagc tcgaagcggc ctttccgcat ggccgcggca ccggtgaacg
     1561 ctcccttctc gtgaccgaag agctcggact cgagcacgct ctccggcagc gcggcgcagt
     1621 tcaccttcac gaaggcttgg cccttacgcg gggataggtc atgtaacgcg cgcgcgaaca
     1681 gctcttttcc cgtcccggac tcgccgcgca gcaacaccgt cgagttgctg cgggccacga
     1741 cacgaatctt ctcgatcacc gagtgcagag ccgtgctgtc gccgacgatg ccgttgagca
     1801 cgctctcctt gcgctccggt acggcctgct cgagctcccg ctccaagcga cgccggtctt
     1861 ccatcaagcg ctcccgatct ctggcgacca gccgttgcag gcgaatggtc tggccgacga
     1921 ggttggcgac catcgtcagg aagcgcacgt cttcgtcgag cgcgaagaag tcttcgggcc
     1981 gcgcttcgcg gtcaatcgtg agcgtgccga tgacacgccc gttgtcgcga atgggtacgc
     2041 cgacgaacga gacgtcgcag ccgacgcccg actcggagaa atcccagttc tcccacagcg
     2101 ggtcttcgcg gatgctgcgc actacgagcg gcatctcggt cgcgacgatg cgaccgatag
     2161 cgcgctccgg aaggtgctcg aagtagcggc gcgcggtcgc ctcgctccag cccgaaccca
     2221 ccacgatttc tggcgcgccg tactcgtcga gcagcgcgat caagccgtgg cgcatgtcga
     2281 ggaagctagc gagcaggtga accacgtgcg ccaacgtgag ttcgaggcga gccggcgagg
     2341 ccagtagctt cgaaatctcg tacacgccgt agagctgcac ttcagcccgg cgcgccgcgc
     2401 taatcgaacg ggtactcccc gttcgttcac cgacgttgac cggctccatg ggggagagat
     2461 cgaaggatgt actcatgcgg agtttccgga aatcttggac acaatctggg gctcttcgat
     2521 gtctaccagt tgcactattg cgggatggcc cccgcagtgt ttcgtgacgc gcgctcgtgt
     2581 aacttgcgcg acgctgggcg acattcgcac tcggccgctt gctcgctcat cacgttccgg
     2641 ctcgctcatg ccgtacgtgg ctcgagctcg tttctggaat tgactcagct gccaaggacc
     2701 atcgaagtct ttagatccat ctatcgacgg gattagcaag cgccgcgcca tcgaaagccg
     2761 agtcacggtc acgcaacggc ggggatcagg tgccgagttc gcttcacggc cgcgctgtag
     2821 ccagcgtttc gccgaatatg gacgcagtct gcacgcatgt cttgtcgcgt ttcttacaaa
     2881 ccgcgcgtgg ttcacgacaa tctccgcaga aattgcgcgt ggcatgcgca atgcttttga
     2941 ggctgcttcg aggcagcagc gccggcagtc gtgcctgctc ggtaaaccta gccgcccgag
     3001 gagcagtccc gtgatcttga tgcaacgccc tccaatcgtg gtcaacgaca gcaccttgcg
     3061 tgacggagaa caggctcctg gggtggcgtt cagaatcgac gagaaggtcg cgatcgcgcg
     3121 agcgctggag gccgcgggcg tggacgaact tgaagctggc atccccgcga tgggcgcgcg
     3181 agaggtggaa gcgatgcagg cggtgcaggc cgcgctcgag aaggcgcgag cgatcgcctg
     3241 gtgtcgaatg accgacgcgg atgtcgaggc cgcacttgcc agcgggctgt cgcgcgtgaa
     3301 cctctcgatt cccgtgtccg accggcagat gcgcgtcaaa ctcggcgttg gaaggcgcgc
     3361 agtgctcggt cggattcaac gcacggtgcg ctacgccatc gaccgcggct tggtggttca
     3421 tgtcggcggc gaagacgcga gccgcgcgga cttggatttc gtatgcagag ccgtggtggc
     3481 ggccgaagag gcaggcgcgg tccgttttcg attcgcagac acggtcggag tgatggatcc
     3541 gttcttgact cacgcggtct ttcaacgtct gtgcgcggaa acggatctcg agctcgagtt
     3601 ccatggccat gatgacctcg ggctcgccac ggccaatacg ctggctgcag tgagaggagg
     3661 cgccactcac gtgagcgtct gcgtactcgg tctgggtgag cgcgcgggca acgcaccgtt
     3721 ggaggaggta gctgcggcgc tcacggccat agaaggccta cgaactggga tcgatcctac
     3781 gcgactgccc gctctcgcag agcaggtcgc cgtcgcttcc ggccgcccca ttccggacgg
     3841 aaaggcaatc gttggacgta gcgcgttcac ccatgagtcg ggcattcacg tggcagggtt
     3901 gctcaaagat cgcctcacct acgaggccct gccgccggaa cgattcggac gctcgcgccg
     3961 catcgtgctc ggcaagcact ctggtcgcgc tgcgatcgat catgcacttc aactcctcgg
     4021 agtggcagcg gacgaccgta gcctcggcgt agtgctggag cgcgtgcggg agcacgccga
     4081 acgaacgaag gccgcaattg gaaacaacga gctgctcgag ttctatgtgg cggcacgcgc
     4141 cagctccgaa caccgacgca gctctcggac gcgcacccgc tgagtgggaa gacactacca
     4201 acttggctag cacttcgaat acccgggttc ggcccatgtc gccgactggc tacatcgaac
     4261 aaccgttgag tgcgcgcttg ttccaaacac ctggccacgt gagccagtaa ggaaggagcg
     4321 cacagcgaga agctcgcatg cgcgattcga atgttgccag tcaagccggt gattcttgaa
     4381 ccgatagaga cgcccccgct ctgtcgggcg agccgcccgc gaaggcacag aagcccgtaa
     4441 ttttgggccg attgcgcatc ggccacgaga ccgacaggca tggcgcgctt cgagcggggg
     4501 ccctgttcga ccggtggcct gttcaagcgg tggtgcgctt cgagcggtgg ccctgttcga
     4561 gcggtggccc tgttcgaacg atggtgcgct tcgagagatc gggtcgcctc gaagccacgg
     4621 cactttgggg aacgccattg gcaattccgg tggtgctcgg cgcgtcgacg ctgaggtaac
     4681 cggtgccacc gcgcgaagcc gtcctgcagc gaagtctgct atcggcgcta ttgatcggtg
     4741 gcccaggcat agcttccaag cgtctgcgct ccgcgagcag agacagagca gtagacccca
     4801 cccgaccagc cgcgcctgca agaggctgcg cgcgcggtaa caccgaagga gggcgatcac
     4861 tcgccccacc aaagacttcg gtagacgctg cgcacgaagc gcacgaaccc acgggtcgtg
     4921 tcgcaaaacc gcgctcccaa ccctgcgcct gacgcttcgt gagcacctcg tagcgcaacc
     4981 tccccgccgg agaactcggc cacccgagaa cactacaacc cgagagcggt gcggtcgcga
     5041 agaaacccag aagcaggcgg actacttcgg aaaacccggg gcttgctcct cgatatcggc
     5101 gcgcgcccga gttaaagtcg cggcaggctc ccgttccagg ttcaaaaaac aaactcaact
     5161 cgagtccaaa atgctccccg cccccggcac gctgaacgtc tcacccgtcg cgcgtcgatc
     5221 tccctccggc gcgcgccggt tccaagaagc gccgccgaaa aaaaatcaga gcggtgcccg
     5281 cgatcgtcgg ctaaggtact cttcggtatt gaactccaag gggccgccgt cacgtcactt
     5341 gcatcgtcgc ggcgcatagc tcaaacccaa gccagacaga atcgcgaccg accaggttaa
     5401 cgtatcagca accagcgagg gcagttacaa cgagggcagc agcagaaccg aaggcactag
     5461 aaccgaaggc actagagcac tgagggcagc acgacgaggg cagcacgacc ggcaggcgag
     5521 cacgtgggct tcgaaagggc accagaacac ttggagccag ctttgaaagc acaagctctc
     5581 gagcacgagc tcggaagaac tcgagcgcga gctcggaaca gctcggaaga acaggtgcag
     5641 tcctcggtca tcacgcaacc gctcgcactc gcggtgtctt gactctcaac gccagggctc
     5701 agcgtcccgt gaatacaagg accatgcgcc agtacctcac cttcttttgc caagccgccc
     5761 cacctcgcaa gcacgagact tccaacccga acactgacta ggcatgaacc gccggctatt
     5821 agacctcgtt tcacgcgttc aggcgagtac agacattccg agtctgctca gtgcggtcat
     5881 ggactgcacg cgagacgtga ccggtgcaca agtggcaggc gtggctgtcc tggtgaacca
     5941 gggctaccag gtgagccagg tgggggcacc agattacttg ttggaaagct atactcagtt
     6001 cacggtctct gccgccgacc cggtccatga ggccctcact caacagcatg gggtgatccg
     6061 tactgacgat ctgtttacgc cgtctgcgtg gcacggccat ccgttctacc agcaggtgga
     6121 agcaccgtcg gggctcgact cctacatggc cgccgagatt gtcgacgcca aaggcgttcg
     6181 tggcttcgtc ggagcgtcgc gcaggaccgg ccaaccgcca ttctcgagtg ccgacgcgag
     6241 cctgcttcaa acggcttgcc tccatatttc cgcagctcta accagattgg gtagcgtcat
     6301 cccgctccag tcgctcactc cgaccgagcg cgaggtcgtg cgcttggttg ctcgtggctt
     6361 cacgaatcag caaattgggc aagcgcgtgg tgtgacagcg catgccgtga agaagaccct
     6421 cgagcggctg ttcgaaaaga tcggggtcac ttcgcgcacg gagctcatcg ccgcgctcgg
     6481 ggtctctacg gagccgtcgc gttgggggcg cgcc
//
LOCUS       DASTKU010000380         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_517685, whole genome shotgun sequence.
ACCESSION   DASTKU010000380 DASTKU010000000
VERSION     DASTKU010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517685"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2876)
                     /locus_tag="VFQ61_13005"
     CDS             complement(<1..2876)
                     /locus_tag="VFQ61_13005"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9955424.1"
                     /translation="MALARSEPRVPPLVLAPGARVEIRGEEWIVRKAEPTSTGDTAVH
                     VTGLSELVRNRNAIFLTALERDLRVLEPEETLLAQDPSPGYRRTRLYLESLLRQSPPT
                     DSRLYRGHRGAMNRAEYQLVPAAKALAQARPRILIADAVGLGKTIEVGVLLSELIRRG
                     RGRRILVVALKSVLEQFQEELWARFTIPLVRLDSLGLERVQRKIPANHNPFYYFDRVI
                     ISIDTLKKDEKYRRFLNDCHWDAVVVDECQHVAVRGGVGPAQRSQRAKLASLLARTSE
                     ALILTSATPHDGKKESFGSLMNLLEPTAIADDTDYTASDVAGLFVRRFKKDVQAQVRG
                     SFQERQIRSLHVDAGSEENAVFELLHTAQFKTIHPRRPRAPNAEPVRETTEPEAATEY
                     RGSGGGILFRTLLLKAFLSSPEALIETVDARLSHKRLVPLSRAGRSDFDTESETDPDA
                     QHDVKLLTTLRRAAEKVVIKKNSKLQRLLQLLGEMGVGRDSRERVVIFSERIATLELL
                     QKQLQPALQLKPQNVAVFHGTLDDQEQQKLVKDFGNEKSSVRVLLASDAASEGVNLHH
                     FCHHLIHYDLPWSLITLEQRNGRIDRFGQTRVPELHYLLTRPANPVIRGDLRVLDVLI
                     DKERAAHENLGDVRWLMNLHDAEAEEDRVAEGISQHEPAEVILPDPEPETDFLATLVS
                     EWQKEARDDHRGRPLEVSSETVLGNDGISPNAVIVPEPCEPLRLFANDLEFAKEAFLE
                     LVESEPSGLPAEARLKAPQWEDQIRGFNLVAPLDLRERYRFLPPELHQRGNDWTFKLT
                     CDRELVQKALERSRQSENGWPEYELFWEQHPIAEWLNDRMAAHFRRHEAPLLSLPRGL
                     TPGECAFVFQGVLSNQRSQPMLVEWFAVIFGLEDGSRVEPLTRLIERVQLTEALVNPG
                     RTLDVTRPTALRGAAVAAAREHMTHLREARRERLTPVLKAGQQRL"
BASE COUNT          517 a          895 c          929 g          578 t
ORIGIN      
        1 aggcgctgct ggccggcctt cagcacgggc gtgaggcgct cgcggcgcgc ttcgcgtaag
       61 tgggtcatgt gttcgcgggc ggccgcgacg gcggctccgc gcagggccgt gggacgcgtc
      121 acgtccaagg tgcggcctgg attgaccagg gcttccgtga gctgcacgcg ttcgataagg
      181 cgggtcaagg gttcgacccg gcttccatct tctaacccga agattaccgc aaaccactcc
      241 accagcatgg gttggctgcg ctggttcgag agcacacctt gaaagacgaa ggcgcattct
      301 ccgggggtga ggccgcgcgg cagggacaag agcggcgcct catggcggcg gaagtgcgcg
      361 gccatgcggt cgttcagcca ctcggcgatc gggtgctgct cccaaaacag ctcgtactca
      421 ggccacccat tctcgctttg acggctgcgc tccagagcct tctgcacgag ctcccgatcg
      481 caggtcaact tgaaagtcca gtcgttgccg cgctgatgca gctccggcgg taagaagcgg
      541 tagcgctctc gcaaatccaa cggagccacg aggttaaagc ctcggatctg atcctcccat
      601 tgcggggcct tcaggcgtgc ctccgcagga agcccgctcg gttcactctc gacgagctct
      661 agaaaggctt ccttcgcgaa ctccaagtca ttcgcaaaga gccgcaaagg ttcgcacggc
      721 tccggcacga tcaccgcgtt cggtgagatg ccatcgttac cgagtacagt ttccgagctc
      781 acctcgagcg ggcggccacg gtggtcgtcc cgcgcctcct tctgccattc cgagacgagg
      841 gtcgcgagga agtctgtctc tggctcgggg tcgggtagga tgacctcggc gggctcgtgc
      901 tggctgatgc cctccgcgac gcggtcctct tcagcctcgg cgtcatgtag gttcatgagc
      961 caacgcacat cacccaagtt ctcgtgcgca gccctttctt tgtcgatcag cacgtccaac
     1021 acgcgcaggt cgccgcgaat caccgggttt gctggacgag tgaggagata gtgcagctcc
     1081 ggaactcggg tttgtccgaa gcgatcgatg cgcccgttgc gctgctcgag cgtaatcaga
     1141 gaccacggta aatcgtagtg aatcaggtga tggcagaaat gatgcaggtt cacgccttcg
     1201 ctggccgcat cgctggcgag tagaactcgc accgagctct tctcattgcc gaagtccttg
     1261 accagctttt gctgttcttg gtcgtccaag gtgccatgaa acacggcgac attctggggc
     1321 ttgagttgca gggcaggctg gagttgtttt tgcaggagct cgagcgtcgc gatgcgctcg
     1381 ctgaagatga ccacacgctc gcggctgtcg cggccgacac ccatctcgcc gagcaattgg
     1441 aggagtctct gaagcttgct gttcttcttg atgacgacct tttctgcggc tctgcggagg
     1501 gtggtgagta gcttgacgtc gtgttgcgcg tcgggatccg tttcagactc cgtatcaaaa
     1561 tctgaacgcc cagctcgaga caggggcacc aatcgcttgt ggctgagccg tgcgtcgacg
     1621 gtctcgatca gagcttccgg agaggacaag aaggctttga gcaggagcgt acggaataga
     1681 atgcctcctc cagatcctct gtattccgtc gcggcctccg gctctgtggt ttcacggacc
     1741 ggttcggcgt tgggcgcgcg ggggcgtcgc ggatggatgg ttttgaactg tgcagtgtgc
     1801 agaagctcga atacggcgtt ttcctccgag ccagcatcga catgcagaga ccggatttgg
     1861 cgctcttgga acgagccgcg gacctgagct tgcacgtcct tcttgaagcg ccttacgaac
     1921 agccccgcga cgtcactcgc tgtgtagtcc gtatcatctg caatcgcggt gggttcgagg
     1981 agattcatca agctaccgaa gctctccttc ttgccgtcgt ggggcgtcgc gctggtcaga
     2041 atcagcgctt cggaagtccg ggccagcaag ctcgcgagct tggcgcgttg cgagcgctgg
     2101 gccggaccaa cgccgccgcg cactgccacg tgctggcact cgtccacgac gaccgcatcc
     2161 caatgacaat cattgagaaa ccgccggtac ttttcgtcct tcttgagcgt gtcgatggag
     2221 atgatgaccc ggtcaaagta gtagaacggg ttgtgattgg cgggaatctt gcgctgcacg
     2281 cgctccaggc cgagggagtc caaccgcacg agggggatgg tgaagcgcgc ccacagctcc
     2341 tcttggaact gctccagcac gctcttcaag gcgaccacca ggatgcgccg gccgcgccca
     2401 cgccggatca gctcgctcaa taggacgccg acctcgatcg tcttgccgag ccccacggcg
     2461 tcggcgatca agatgcgagg ccgcgcctgg gcgagcgctt tggccgcggg caccaactga
     2521 tattcggcgc ggttcatcgc gccgcggtgg ccacggtaga ggcgcgagtc cgtggggggt
     2581 gattgacgga gtaggctctc cagatagagc cgagtacgtc ggtaacccgg cgaaggatcc
     2641 tgcgcgagca acgtctcttc cggctcgagt acccgcaggt cgcgctcgag ggccgtcaga
     2701 aagatggcgt tccggttccg aaccagttca ctcaaaccgg tcacgtgcac cgcagtgtcc
     2761 ccggtcgacg tgggctcggc ttttcgaacg atccactcct cgccacgaat ctcgacccgc
     2821 gcgcccggcg ccagaaccaa tgggggaact cgtggctcag aacgagcgag tgccatgctt
     2881 ctcctggagc gacgctcctg ttccctgccg gcgattcga
//
LOCUS       DASTKU010000381         7457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1632754, whole genome shotgun sequence.
ACCESSION   DASTKU010000381 DASTKU010000000
VERSION     DASTKU010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632754"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1055..1897
                     /locus_tag="VFQ61_13010"
     CDS             1055..1897
                     /locus_tag="VFQ61_13010"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9955425.1"
                     /translation="MIIDTHTHFYDPSRPAGVPWPSVNDALLYRPFLPEEYARVIKPF
                     GVVGTVAVECNRPVEQDNAWVLEQADRNAILQAFVGYLDPAAPWFQNELAGCLKHRAF
                     RGLRIHSRDVPSILSSAGGTRGLEVLAECGGVVEVISGRETYANTLALCRAVPKLTVV
                     LNHLGKTCFDDPEQELWQTLRRLASYRNVVAKVSGLLEDAPLDSRSELVYRAGVRRCY
                     EVFGEERLVFGSNWPVTLRYASLEELFRFALELLHSLPATAASAVLLHNAQRVYRLNG
                     HPLG"
     gene            1930..4035
                     /locus_tag="VFQ61_13015"
     CDS             1930..4035
                     /locus_tag="VFQ61_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HET9955426.1"
                     /translation="MGRRVIPVEQMEVAECGIACLAMVLGYHGAFLRLDELRRVCGTS
                     RDGNSALDLVNAAQSLGLDAIGKKAPVDYLREAQLPCILHWQMNHFVVLEGFRKNRAK
                     IVDPALGRREVGPEELHSSFTGVLLEFSRTEQLRRRKRPALGYGRYVKAVLSRPNAVV
                     FVLSIAVARQILAVVYPTLTRVLVDEVIRPQRQRWLLPVLGVLVVTTVLQLILLRYQS
                     RCLARLHATLGAELSGKLGEHLFRLPLEVLMSRQHGDLLERVQQHGLLKSQMTDALTG
                     FLKLMFLVSLAGLMLVYSPGLAAVVLAVSALRVAILLAVRRPLRQLATEELSAAGRER
                     GCLLEATAGAELLRGFRAEERVQARYTKRLAKRINCSIRAQRFRSGSEHWLSTFGGLT
                     LAGALAFGGQLVTTGQMTPGVFAAFVAMLNLWEEPLGAVVSSVENWIRCRSILERCDD
                     ILSTPRVESPESGPTAVHGHLVLRDVGYRYGTRGPWLFRNVTLELRPGEHCAIVGPSG
                     HGKSTLAKLLCGLLLPTEGQVFIDGVEPQRFPSDVLARDFGVVLQGPVIVDGTLGENV
                     SVRVPGLDQSGIHDALRTCCLEHVVGQLTRGVHTPLRAEQPIFSGGEMQRLALAQAVA
                     GRPRMLLLDEATSSLDADTEAHVMANLRELDATIVSVAHRPGAVRGADRLLVVAGGAV
                     QEFDRSYAGMDLSFEQVGA"
     gene            4032..5678
                     /locus_tag="VFQ61_13020"
     CDS             4032..5678
                     /locus_tag="VFQ61_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9955427.1"
                     /translation="MSASAGLDGIADFPPGASFRRMLTALLKQERRVFYKLSLASGVG
                     ALLALAPPLAVQYAMDDAVPNRKPQLLVLLAVAVCVLTFNSAWVGWIELSTQVLFKNH
                     AEARCLRSLLGRLLRAPFAKIQEKDSGWALETLRKAAQIVDSAATSVVSLLTRALTSL
                     SSFVLLAIWFPALAAACALGMTLMITASMWIAHVESTRRLKLLQASGDQVLFLHTLLR
                     SLTSVRALGATRRFGARWQTLLEREARAELQVTLAELDRSQLLDAGQRFLAVAVNCIL
                     AFRCVAGVTSVGVMMTSTLLLGNVLQSFVSISGTLLEFVALRSQFARVDELIRLQPDR
                     DAGQSVVAQSAGAQSAGAQSAGALHQIRLDRVFFRYPEASRWVLQDYSCHFEIEGFHR
                     ISAASGAGKTTLLRLIAGLLTPQSGAVRIGGREVADVSQLVLYSPQLNALLEGSLGDN
                     LRLLSGRDLEHVMAVAEATGLSQLVRTLPMGLDTLITAGGANLSAGQRQLVILTAAFA
                     SDKPILLLDEPTSQLDSTTRAAIQWEWLARSKTVLLVEHH"
     gene            5764..6264
                     /locus_tag="VFQ61_13025"
     CDS             5764..6264
                     /locus_tag="VFQ61_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955428.1"
                     /translation="MTDIQDDKLPYVACAAFCELDSKVLMLLDEGNDPSEGRDNARGE
                     RRCGNQVLAQPVGRLQPREDLIQATARIVRADTGLEISIHSMLDPYVWLMTNGYSIIR
                     FCFIAQVVGGRLTGQGSCKPTWYSQGELREHEGLLRNPATVRAVSDYISGVRHPLDRV
                     QTFRCW"
     gene            6633..>7457
                     /locus_tag="VFQ61_13030"
     CDS             6633..>7457
                     /locus_tag="VFQ61_13030"
                     /inference="COORDINATES: protein motif:HMM:NF015150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HET9955429.1"
                     /translation="MRVDARPAISIAEFRRGSEDERLAIAMRAHELYERERYLLIVDH
                     GIDDAILSQMPGVAEPFFRSPLSTKLRCLARSGLGYLPSPDSMNLPVANNPNAPHEQF
                     IAHLDAAKNPWPTELPGFAETVSVYNRACEQLCHVLAEMMALGCGVPVSHVREREEAP
                     NASFLKFIYWRASPGSRRGEYRLAPHHDNAAVSLLKNPERANGLRVQRRDGTWQEAYA
                     FPGALVVLFGEQMANWTEGRVTPSWHQVVSGEAELGGPAEPGGPAEPGGPAEPGGPA"
BASE COUNT         1309 a         2134 c         2488 g         1526 t
ORIGIN      
        1 ggccgcaaaa cgattttgcg cgccgatggc ataattttcc ccagttcacg aggtaaggcc
       61 acgccgggcc ctgcgcggca gatctgcgcg ggttcaggca cgcgcagatc tgccgcgcta
      121 accggcgact ggtggacaca atcagcgcgc ccaaacttgc tcgtcgaccg gggcccgcgc
      181 ggtgcggagc tgtcaccggc gcgtcgagcc ccctcgccca cgcccctcag tcgagttcct
      241 tcaatgcggg aattcggaat cacatgttcg agcctctccg agcgcgggta ttgggaggcg
      301 ggccagcgct cagcgcgcat aacccggctg attccgtgtt tggggacgag tcgagtcgaa
      361 agcaagagaa agacacgtca gttcgagctc gtgagttgaa gtcgagagcg acccgcacgc
      421 ttcagtacag gtcatggcga aattgccgat tgaccgacta taggctgagt gttattcatc
      481 caagagcgcg ggttaaaggt atattcgttt ggaggctcgt tgaaagatcg ctaaaaggac
      541 cgctgtattt gcgagcgtcg agtcgaaaca gttgggtgta aagcacaggg gtatgaggca
      601 ctgggtgtga ggcacgtgga tatgcctgct ggcatggata caagttgcgt cgatacaggc
      661 gatagcgacg agccatagct cgtcgacagg cgatagcaac gagccatagc agcagtcaaa
      721 gtagtcccgg ttgctccggc gttgggtggt ggcgcgcgta gcttgggtat gcgccgaggc
      781 cttcgaggtg tggatgatcg gcaaacctca agtgtacagc tcgtggtgag ctttgggttg
      841 aacgttcatg agagagaatg atgcgagagg aacagacctc tcggtggttg ggttgcttcg
      901 gtgaatcttc ttcttccggc aactagggag ttcgtggggt gacagattat gatcgatcgg
      961 gctgaatgcg cgtcacgaga ggtggtactt ccgtgagcgc caacgatcgt cgtgctggtt
     1021 ggggcagacg cagagttcga gtgaagcgga accgatgatc atcgatacgc atacgcattt
     1081 ctacgatccg tcgagaccgg cgggggtgcc gtggccatcc gtaaacgatg cgctgttgta
     1141 tcggccattc ctacccgaag agtatgcgcg ggtcataaaa ccgttcgggg tcgtcggaac
     1201 cgtggcggtc gagtgcaatc gacccgtcga acaggacaat gcttgggtat tggaacaagc
     1261 ggaccgcaat gccatcctcc aggcgttcgt gggctatctc gatccggcgg ctccttggtt
     1321 tcagaacgaa ctcgcgggct gcttgaagca ccgggctttc cggggtctac ggatccactc
     1381 tcgagacgtg ccctcgattc tatcctccgc cggtggtacc cggggcctcg aggtgctcgc
     1441 cgagtgcggc ggcgtcgtgg aggtgatcag cggtcgcgaa acttatgcga atacgctcgc
     1501 gctgtgccgg gctgtcccca agctcacggt cgtgctcaat catctgggaa aaacctgctt
     1561 cgacgatccc gagcaggagc tgtggcagac cctccgtcgt ctcgcatctt accgcaacgt
     1621 ggtggctaag gtctccgggc tactggaaga cgcgccgctc gactcgcgga gcgagctcgt
     1681 ttaccgcgcg ggcgtgcgtc gctgctacga agtgttcggc gaagagcggc tggtgttcgg
     1741 ctccaattgg ccggtcacct tgcgatacgc ctccctcgaa gagctgtttc gatttgcgct
     1801 cgaacttcta cacagtctgc cagcgacggc cgcaagcgcc gttctgctcc acaacgccca
     1861 gcgggtgtac cggttgaacg gtcacccgct ggggtgaagc caacgagccc gagagaaagg
     1921 agaacgaggt tggggcgccg agtcataccc gtcgagcaaa tggaagtggc cgaatgcggc
     1981 atcgcgtgct tggcgatggt ccttggctat cacggcgcct tcctgcgctt ggatgagctt
     2041 cgccgggtgt gcggcacttc gcgcgacgga aacagtgcgc tcgacttggt caacgccgcg
     2101 cagagcttgg gcctcgatgc gatcggtaag aaggcaccgg tggattacct gcgggaggcc
     2161 cagctaccct gcattctgca ctggcagatg aatcacttcg tggtgctcga aggttttcga
     2221 aagaaccggg caaagatcgt ggatccggca ctgggccgcc gcgaagtggg ccccgaggag
     2281 ctccactcgt cgttcacggg cgtgttgttg gagttctctc gaacggagca gctgcgcagg
     2341 cgcaagcgcc ccgccctcgg ctacggacgc tatgtcaagg cggtgctcag ccgaccgaac
     2401 gcggtcgtgt tcgtgctttc gatcgccgtg gcgcgacaga ttttggccgt ggtttacccg
     2461 acgctcacgc gcgtgctggt ggacgaggtg attcggccgc aacgtcaacg atggctcctt
     2521 ccggtgctgg gtgtcctcgt cgtcacgacc gtgctgcagc tgattttgtt gcgttatcaa
     2581 agccgctgct tggcacggct tcacgccacg cttggcgcag agctgtccgg caagctaggc
     2641 gagcacctgt tccggttgcc gctcgaggtc ttgatgagcc ggcagcatgg tgacctgctc
     2701 gagcgcgttc agcagcatgg gctgctcaag tcgcagatga cggacgcgct gacgggcttc
     2761 ttgaagctca tgttcctggt ctcattagct gggctgatgc tggtgtactc gccgggcctg
     2821 gccgcggtcg tgcttgccgt gagcgcgttg cgggtagcca tactgctggc ggtgagacgt
     2881 cccctgcgcc agctcgcgac cgaggagcta tcggcggcgg ggcgcgagcg cgggtgcttg
     2941 ttggaggcca cggccggcgc ggagctgttg cgtggcttcc gcgccgaaga acgggtccag
     3001 gctcgttata ccaagcgcct ggcgaagcgg atcaactgct cgatccgcgc acaacgcttc
     3061 cgttctggtt cggagcattg gctatccacc ttcggtgggc tcaccctggc cggcgcgctc
     3121 gcatttggcg ggcagctcgt gacgacgggg cagatgaccc ccggtgtgtt tgccgctttc
     3181 gtcgcgatgc tcaatctgtg ggaggaaccg ctaggcgcgg ttgtctcgag cgtcgaaaac
     3241 tggatccgtt gccgcagcat tctggagcgc tgcgacgaca ttctgagcac tcctcgcgtc
     3301 gagtccccgg agagcgggcc caccgccgtt cacgggcacc tcgttctacg ggacgttgga
     3361 tacagatacg gcacccgcgg gccgtggctc ttccgcaatg taacgttgga gcttcgcccc
     3421 ggggagcact gcgcgatcgt cggaccgtcc gggcacggca agtcgacttt ggccaagctg
     3481 ctctgcgggc tgctgctgcc tacggaaggt caggtattca tcgatggcgt ggagcctcag
     3541 cgcttcccgt ccgatgtcct ggctcgggac ttcggcgtgg tgttgcaggg cccggtgatc
     3601 gtggatggta ctctagggga gaacgtgtcg gtgcgcgttc ccgggttgga ccagtccgga
     3661 atccacgacg cacttcgaac ctgttgcctg gagcacgtcg tgggccagtt gacgcgtggc
     3721 gtgcataccc cgctgcgcgc cgaacagccc atcttctcgg gcggagaaat gcagcgtttg
     3781 gctctggccc aagcggtcgc aggccggccg cgcatgctgt tgctcgacga ggcaaccagc
     3841 tctctcgacg ccgacaccga agcgcacgtg atggccaacc tgcgtgaact cgacgcaacg
     3901 attgtgtccg tcgctcatcg gccgggcgcc gttcgcggcg cagatcggct gctcgtggtg
     3961 gccggcggcg cagttcagga gttcgatcgc agctacgcgg gcatggatct cagcttcgag
     4021 caggtgggcg catgagcgcg agtgcgggtc tggacggaat tgctgacttt ccgccgggag
     4081 ccagcttccg gcgaatgctg acggcgctcc tgaagcaaga acgacgtgtg ttctacaagc
     4141 tgagtctcgc gagcggcgtg ggtgcgctgc tcgccctggc gccgcccttg gccgttcagt
     4201 acgcaatgga cgacgcggtc cccaatcgca agccccagct gctcgttctg ctcgcggttg
     4261 cggtctgcgt cctgaccttc aactcggcct gggtcggttg gatcgagctg tcgacgcagg
     4321 tgctgttcaa gaatcacgcg gaagcgcgtt gcctgcgcag cttgctcggc cgtttgctgc
     4381 gcgcgccgtt cgcgaagatc caggaaaagg actcaggctg ggcgctcgag acactgcgca
     4441 aagcagctca gatcgtggac agcgccgcga cgagcgtggt ctcgttgctc acgcgcgcgc
     4501 tgacgagcct ctcctccttc gtgctgctgg cgatttggtt tcccgcgctc gcggccgcgt
     4561 gtgctctcgg catgacgctg atgatcaccg ccagcatgtg gatcgcacat gtcgagtcga
     4621 cgcgacggtt gaagctgctg caggcttccg gtgaccaggt gctcttcttg cacacgcttc
     4681 tgaggtctct aacctcggtg cgagcgctcg gcgcgacgcg ccgcttcggc gcccggtggc
     4741 agactttgct cgagcgcgaa gcgcgcgccg aactccaagt gaccttggcc gagctcgatc
     4801 gaagtcagct actcgatgcc gggcaacgct ttctggcggt ggccgtcaac tgcatattgg
     4861 ccttccgttg cgtggccggc gtgacctcgg tgggcgtgat gatgacgtcc acactgctgc
     4921 tgggcaatgt tttgcagagc ttcgtttcca tctcgggcac gctgctggag ttcgtggctc
     4981 tgcgttcgca atttgcgcga gtggatgagc tgatccgctt gcagcccgac cgcgatgcag
     5041 gccaatcggt tgtagctcag tcggctgggg ctcagtcggc tggggctcag tcggctgggg
     5101 ccctccacca gatacgactc gatcgagtat tctttcggta tcctgaggct tcacgctggg
     5161 tactgcaaga ttactcctgc cacttcgaaa tcgaaggttt tcacaggatt agcgccgcat
     5221 ctggcgcggg caaaacaacc ctcttgcggc tgattgccgg cttgctgacg ccacaaagcg
     5281 gggcggttcg gattggagga cgagaggtcg ccgacgtgag ccagctcgtt ctctattcgc
     5341 ctcagctgaa tgctctcctg gagggcagtt tgggagacaa cctgcgcttg ctgagcgggc
     5401 gagatctgga gcacgtcatg gctgtcgccg aagctaccgg tcttagccag ctcgttcgga
     5461 ctctacccat gggactggac actttgatta cggccggggg agcaaacttg tccgcagggc
     5521 agcggcagct cgtaattttg accgcggcgt tcgcctcgga caaaccgatc ttgctcttgg
     5581 atgaaccaac gtctcagcta gattcgacga cacgcgccgc gattcaatgg gagtggttgg
     5641 cacgctcgaa gacggtgctc ttggtcgagc atcactgaac ggcttcgcgt tgccacacga
     5701 acgcgaggaa tcgagcttag aaatcgcgtt cacgggtgca gatagggaag aaggtgagga
     5761 tcattgaccg acatacaaga cgataaactg ccgtacgttg cctgcgccgc gttctgcgag
     5821 ctggattcca aggtgctcat gctcctcgac gaaggaaatg atccctcgga ggggcgcgat
     5881 aacgcacgtg gagagcggcg ctgcggaaat caggtgctgg cacagccggt ggggcgcctt
     5941 cagccccggg aggatctgat acaggccacc gcccgcatcg tgcgcgcgga caccggtctc
     6001 gaaattagca ttcacagtat gctcgatccg tacgtctggc tgatgaccaa tggctactcc
     6061 atcatacgat tttgtttcat tgcccaagtc gtgggggggc gcctgacggg gcaaggctcg
     6121 tgtaaaccca cttggtatag ccaaggcgag ctgcgggaac acgagggcct cctgcgaaat
     6181 cctgccacgg ttcgcgccgt ctccgactac atatcgggag tacggcatcc attggatcgg
     6241 gttcagactt ttcgttgttg gtagtgcggg gtcgaccacg gcgcggggtg gaacgtaagt
     6301 cggttagtcc gtgagccgtt gggtgagttg ttggtgatct cggtcccgtg ccgggtttca
     6361 acttgacgac acgatacgag aacggtactt tgcaaatggg agctggttgc ggtgttatga
     6421 atggggtctg agacgagtct ctcggatctg ggcggtatcc aagtcagaat agttcgtgaa
     6481 acgaatctcg ggtctggtac gcccagcgtt gatacctggg tgtggtcgat gggtcgtttt
     6541 ggcgagtcgc gtctttgctt aggcaacagc gcgggtctga agggagcagc tgtgggcggt
     6601 agcggcggtg cacctcatcg aaggagagca ggatgcgtgt agatgcgcgt cccgcgatat
     6661 ccatcgcgga gttcaggcgt ggttcagaag atgagcgctt agcaatcgcg atgcgggccc
     6721 atgagctcta cgagcgcgag cggtaccttc tgatcgtaga tcacggcatc gatgatgcga
     6781 tcttgtcgca aatgccgggc gtggcggagc cgttcttccg ctcgccgcta agcacgaagc
     6841 tgcgctgcct cgctcgctcc gggctgggct acctgcccag tcccgattcg atgaatctac
     6901 ccgtcgccaa caatcccaac gcgccgcacg agcagttcat cgctcacctg gacgcggcga
     6961 agaatccatg gcccacggag ctgccgggat tcgcagagac cgtcagcgta tacaaccgcg
     7021 cgtgcgagca gttgtgtcac gtgttggcgg agatgatggc gctcggttgt ggagtgcccg
     7081 tatcgcacgt gcgagagcgc gaggaggcgc ccaacgcctc atttctgaag ttcatctatt
     7141 ggcgggcgtc accgggatcg cggcgggggg aataccggct cgcccctcat cacgacaacg
     7201 cggctgtttc gttgttgaag aacccagagc gtgcgaacgg tctccgcgtc cagcgccgag
     7261 atggaacttg gcaggaggct tacgcctttc cgggtgcgtt ggtcgtgctt tttggcgaac
     7321 agatggccaa ttggacggaa ggtcgcgtga cgcccagctg gcatcaagtg gtgagtggag
     7381 aggccgagct gggcggacca gcagagccgg gtggaccagc agagccgggt ggaccagcag
     7441 agccgggtgg accagca
//
LOCUS       DASTKU010000382         3450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_517972, whole genome shotgun sequence.
ACCESSION   DASTKU010000382 DASTKU010000000
VERSION     DASTKU010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517972"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFQ61_13035"
     CDS             complement(<1..341)
                     /locus_tag="VFQ61_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955430.1"
                     /translation="MSSDDAPTALRAVLAPAGGIWKAAAPPLNAADSSAHGSQGERKG
                     SNAQHNGSNGGAAAGEPGVSAGAPEGSHLVVEHLVRQFGDYVALSDVNLSIGRGHVAV
                     IIGGSGAGKTTL"
     gene            369..656
                     /locus_tag="VFQ61_13040"
     CDS             369..656
                     /locus_tag="VFQ61_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955431.1"
                     /translation="MTEEHLSALLSAAEAKKNDQGFLQPSEGRTITLYMASGGASLQV
                     SKIEALRTERDLVHARTTKGELFVLALVDVFAGSVDGPSSGGRKAGFAAER"
     gene            896..1675
                     /locus_tag="VFQ61_13045"
     CDS             896..1675
                     /locus_tag="VFQ61_13045"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET9955432.1"
                     /translation="MSEEDLLRLGSRTFRSRLIVGTGKYASVEQTRLALEATGAEIIT
                     VALRRVDLKDRGPGSLSELLMSGRYTLLPNTAGCYTADEALRTLRLARELGIANLVKL
                     EVIGDPKTLYPDNEQTLEAAKQLVKEGFTVLPYCIDDPIVCRKLEDIGCAAVMPLAAP
                     IGSGLGIRNPYNLRIILENSKVPVIVDAGVGIASDAAIAMELGCDGVLMNTAIAGAKN
                     PVLMAEAMREAVSAGRKAFRAGRMPRRLYANASSPESGLIE"
     gene            1810..3015
                     /locus_tag="VFQ61_13050"
     CDS             1810..3015
                     /locus_tag="VFQ61_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9955433.1"
                     /translation="MSQAFTSYQRRLFVFLSVACFFEGYDFFALGQLLPNLRAHFGLS
                     PSQGAAMVGVVNAGTVLAYAMVGLADRWGRRRVLTTTILGYTLFSLFSGLAPNAVVFT
                     LCQLVARVFLIGEWATSMVIAAEEFPAERRGMVIGVISAVTSLGSIFCAGLVPTLLRT
                     PLGWRSVYLAGTLPLLLIAYARRGLRETARFQESRTPPPGLTGLVRTKSFRRVLVLGA
                     VWFLCYVCSQNAIFFWKEFALAERGMSDADVGKMVVVAALSAMPFAFASGVLLDKVGR
                     KIGTILILSLLSLGVFIGYTAHSKAALGLALALATIGANSMLSALNTLTTELVPTEQR
                     GAAFAWANNLIGRVGYWLSPFAIGLLVEDVGWGNVLRWTAFFPLAACVLIAILLPETK
                     GRELEETAA"
     gene            3012..3413
                     /locus_tag="VFQ61_13055"
     CDS             3012..3413
                     /locus_tag="VFQ61_13055"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET9955434.1"
                     /translation="MSLLLRQPWLRAVPAVVYAGLVFYGGTAQAPLLPPTPVLSSDKV
                     LHGLAFAGLEGLFELWGLELQAKARRVAAVCASIALGGLLELVQLALPYRSADWFDWM
                     ADAIGAVFGALFITLLARTALARGLGAWLWH"
BASE COUNT          540 a         1007 c         1203 g          700 t
ORIGIN      
        1 agggtggtct ttcccgcgcc cgagcccccg ataatgaccg cgacgtggcc ccgaccgatg
       61 ctcagattga cgtcggaaag cgccacgtag tccccgaact gacgcaccag gtgctccacc
      121 accaagtgcg aaccttccgg cgcccctgcc gagactccag gctcgccggc ggccgcaccg
      181 ccattggagc cgttgtgctg ggcattggat cccttgcgct ctccctggga gccgtgggcg
      241 gaagaatcgg cggcgttcaa tgggggtgcg gcagctttcc agataccacc ggcaggagca
      301 agcaccgcgc gaagggccgt cggcgcgtcg tcgcttgaca tgtggcgggg actttccatg
      361 ctccggccat gaccgaagag cacctgagcg cattgctgtc agcggccgag gcgaagaaga
      421 acgaccaggg attcctgcag ccctcggaag ggcgcaccat caccctgtac atggcgtcgg
      481 gcggagcgag cctccaagtc tccaaaatcg aggcactgcg caccgagcgg gatctggtgc
      541 atgcgcgcac gaccaaagga gaactattcg ttctggccct agtcgacgtg tttgcgggtt
      601 ccgtcgacgg tccgagctcg ggtgggcgta aggcaggttt tgccgcagag cgctgagccc
      661 aattgaaggg cgctgagggg aaccgtcgct gggctgctgc cgcaggcgtt cacgcatcgg
      721 gcaacttggc gcgggacagg ctgaggtcgc gcttggaccg gggagggtgg cggtctgatg
      781 attggctcac cgcgggcggg ctcggtgagc cggcgagcgg ccacggcttc gtgcatcgcg
      841 ggggaaatgc ctcgtgagtc ttgccgcgga ggaatcgcgg cggtacgggg ctcgtatgag
      901 cgaagaggat ctgctgcgcc taggttcccg gactttccgg tcgcgactca tcgtgggcac
      961 cggcaaatat gcgagtgtcg agcagacgcg tttggcgctg gaggcaacag gcgcagaaat
     1021 catcacggtc gcgctgcgtc gggtcgatct gaaagatcgc gggccgggca gcttgtccga
     1081 gttgctgatg agcggtcgct acacactgct gccgaacacg gccggttgct acacggcgga
     1141 cgaggcgctg cgcacgctgc ggctcgcgcg cgaactcggc atcgcgaacc tcgtcaagct
     1201 ggaggtaatc ggagatccga aaaccttgta tccggacaac gagcagacgt tggaggcggc
     1261 caagcagctc gtcaaagagg gtttcaccgt gctgccatat tgcatcgacg acccgatcgt
     1321 ttgccgcaag ctggaagaca tcggctgcgc ggcggtgatg ccgctcgcgg ccccgatcgg
     1381 atcgggcctg ggcatccgca acccctacaa cttgcgcatc atcctcgaga actcgaaggt
     1441 gcctgtgatc gtcgatgcgg gcgttggaat agcgagtgac gcggctatcg cgatggagct
     1501 cggctgcgac ggggtgctca tgaacaccgc gattgcgggg gcaaagaatc ccgtgctgat
     1561 ggcagaagcc atgcgggaag ccgtgagcgc tgggcgcaag gcgtttcgtg ccggccgcat
     1621 gccgcgccgt ctgtacgcga atgcgtcgag cccagagtcc gggttaatcg agtagcgcct
     1681 tggtgtgggg acgcggctcc tcgacgcggc ggagccattt tcttttacgt tgaatccggt
     1741 agagcctgtc tcaatcatcg ggccgggctc tgaggcgtaa cgcagttatc gaactcggtg
     1801 agttggatct tgagccaggc tttcacgtcg tatcagcggc gtctgttcgt gttcctgagt
     1861 gtggcgtgct tcttcgaggg gtacgacttc ttcgcgctcg gccaattact tcccaatctg
     1921 cgcgctcact ttgggctgag cccgtctcag ggagcagcaa tggtcggtgt cgtcaatgcg
     1981 ggaaccgtgc tggcgtatgc catggtgggt ctcgcggatc gttggggaag gaggcgcgtg
     2041 ctcaccacga ccatcctcgg ctacacgctg ttctcgctgt tcagcggcct ggcgcccaac
     2101 gccgtggtgt tcaccctgtg tcagctcgtg gcgcgtgtat tcctgatcgg cgagtgggcg
     2161 acgagtatgg tgatcgcagc cgaagagttc cccgccgaac gcagaggaat ggtgatcggg
     2221 gtgatcagcg cggttacgag cttgggctcg atcttctgcg ccggtctggt gccgacgttg
     2281 ttgcgcactc cgctcgggtg gcgctctgtt tacctggccg gcacgctacc gctgttgttg
     2341 attgcctatg cgcgccgcgg gttgcgtgag acggcgcgtt ttcaggagag ccgcactcct
     2401 ccgcccggtc tcaccgggct cgtgcgcacg aagtctttcc gtcgcgtgct ggtcttgggg
     2461 gcggtttggt tcctttgcta cgtgtgctcg cagaatgcga tcttcttctg gaaagaattc
     2521 gctctcgcgg agcgtgggat gagcgacgcg gacgtcggga agatggtcgt ggtagccgca
     2581 ctctcggcca tgccgttcgc gtttgcctcg ggcgtgctgc tcgacaaggt gggtcgcaag
     2641 attggcacca tcctgatctt gtcgctgctc tcgttgggtg tcttcatcgg atacacggcc
     2701 cacagcaagg ccgcattggg gctggcgttg gcgctcgcca cgattggcgc caacagcatg
     2761 ctgagcgcgc tgaatactct tacgaccgag ctggtaccga ccgaacagcg cggggccgcc
     2821 tttgcttggg ccaacaactt gattggccgc gtcggctact ggctatcgcc gttcgcgatc
     2881 ggtctattgg tggaagacgt aggttggggg aacgtgctgc gttggacggc attctttccg
     2941 ttggcggcct gcgttttgat cgccatcctg ctgcccgaaa cgaaggggcg tgagctcgag
     3001 gaaaccgcgg cttgagctta ctgttgcgac agccgtggct gcgcgccgtc ccggcggtgg
     3061 tctacgcggg tttggttttc tacggaggca ccgcgcaggc gccgctcctg ccgccgacgc
     3121 cagtcttatc gagcgacaag gtgttgcatg gtttggcgtt cgccgggctg gagggattgt
     3181 tcgagctttg ggggctggag cttcaggcga aggctcggcg cgttgccgcc gtctgcgcga
     3241 gtatcgcgct gggcggccta ctcgagttgg ttcagctcgc attgccttac cgcagcgcgg
     3301 actggttcga ttggatggcg gacgccatcg gcgcagtctt cggtgccttg ttcatcacgc
     3361 tcctggcgcg cactgctctg gcgcggggat tgggggcatg gctgtggcac tgacctgcgg
     3421 cggcctgcgg cggcactgac ttgggatgca
//
LOCUS       DASTKU010000383         9030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1186693, whole genome shotgun sequence.
ACCESSION   DASTKU010000383 DASTKU010000000
VERSION     DASTKU010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186693"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VFQ61_13060"
     CDS             complement(<1..781)
                     /locus_tag="VFQ61_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET9955435.1"
                     /translation="MRSPLLGLGFWLLSACSSTGTPLPSEGSGGSGPAGSPGIGGSTS
                     SGGSSSGGASAAQGGASSGGLSTTGGLSSATGGSGSGGSPGASGGSVSSGGSAGLSGG
                     SGGSAGLGGASSSGGSTANTGGGVNGGTAGAAAGAGGSTQGSGGSAPGSAGSSGATMK
                     GGVPDSVISQATKAADLVAAWKLGWNLGNSLDSNGCGAAGAETCWGNPMVTQGMLTYV
                     TSLGFNVVRIPVTWSEHLGPSPSFTIDATWMARVDTVVNYAI"
     gene            complement(806..973)
                     /locus_tag="VFQ61_13065"
     CDS             complement(806..973)
                     /locus_tag="VFQ61_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955436.1"
                     /translation="MSDAKVVEGLRPVANREQNKLRRGRSVLLGGAGVPRWNVHSTAL
                     VSLAELAGLSR"
     gene            complement(980..2083)
                     /locus_tag="VFQ61_13070"
     CDS             complement(980..2083)
                     /locus_tag="VFQ61_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955437.1"
                     /translation="MSTSTDTNTMSGGAGAGASTAGLGGSGPGVIGGTAAGGASGRVG
                     AESGGRATDGGQAGAGAAGSQSGGVTGEGGRTASAGMSSTGGRSSNERSISFGTLLDA
                     EAVIRAAQALAQAQANPNAAEWRSKGDQHRSYRFAEADTTAPYRLYVPTTWDGQSMLT
                     LVMFLHGSGANENTYVDQNNKQLINLAQQGGFVLVAPLGDKGAYGNFLRLAAPFGKPD
                     EAAKLMAQVTADSEKTNELSERDVINVLELVLNEYPINRGAVFLTGHSMGSGGTWYIG
                     GKYAKYWAGLAPMSGPFVQESGYPWESVRPISLFVTEGTQAPSLEGSRALRTWLDKNG
                     FKVEYKEVNADHGGMVSLVLPSVFDFFVSLNAK"
     gene            2574..2837
                     /locus_tag="VFQ61_13075"
     CDS             2574..2837
                     /locus_tag="VFQ61_13075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HET9955438.1"
                     /translation="MSSSIHGHEVLEMMVTAGIPFTSASLRDAIIQRFGVEARFHTCS
                     ARDMDAAALVDFLSARGKFVPVSDGFVFSGKSCSHGDDADHHH"
     gene            complement(2847..3671)
                     /locus_tag="VFQ61_13080"
     CDS             complement(2847..3671)
                     /locus_tag="VFQ61_13080"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HET9955439.1"
                     /translation="MLSTSVAAAEAPLLQSFPMLEGRAAQAWRHQPAFRRPRHFHPEP
                     ELNLVLRGHALIRVGELRIEAGPGALLCFQPGQDHELEYASDDLDLFVLALRPELAAK
                     ISSWPSAHAIQPVKLSTQETNQLGNRLAASSELRDRNAVEGLIADVFERMGPRLRQAH
                     VVSRRAMGELTRDPSASERELALLARSNPSQVSRLFKRDLGLRLVEYRARQRLMHFID
                     GVDAGQSFTQAALEAHFGSYAQCHRVFQRIMRCAPRDYFAGARTHVDARLALDALK"
     gene            3870..4685
                     /locus_tag="VFQ61_13085"
     CDS             3870..4685
                     /locus_tag="VFQ61_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET9955440.1"
                     /translation="MRMVLCAGLALVCAACGASSPSAAPEQGGEPPQSIGTFRLLYRD
                     DFDTLDASRWQAMTHSWDSNLALFSSDSVAVQDGKLQLSLLPAPTGTKDSSGATKSFL
                     GAEVRSRDTLTYGRVRARIKFARGPAVISSLVTIYTPWPADNWNELDIECLGKAPQSL
                     QFNTQVYLGTLPAASTPVSPSPAEKKVDLGFDPSEDFHDYDIEWTPSGARFSVDSRVL
                     HTWTQHIDLMKLPQNVLLTIWASSSAGWAGAVGPETSSAKAYYDWVELYRYEP"
     gene            4682..5890
                     /locus_tag="VFQ61_13090"
     CDS             4682..5890
                     /locus_tag="VFQ61_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET9955441.1"
                     /translation="MSGWMPRARRVAWCAPVLVACTSANPMQLGGAGASSSAGVANAS
                     GGAGQSASSGGGATAAGSPSGGTGAVGTSTTGTGTAAGGALPTGGSRGDEQHSGGNSS
                     SGAQQGQGGARSSTTAAGGIANASGGASSSGGQASSAGTPNGGAGAPMGGTSASGGSS
                     QAGAAANGGASDPFTLSWQDEFETLDTARWQLQNFTWEGNQAQFTPQNAAVANGILTL
                     SLTTAESGAAKPYLGVEMRSAKTLLHGKVSARMRFAKGPGVVSGLVLFYTPYPNCDWN
                     EIDIEHLGNSSTTSQLNAMVFTGTPMANCTTSVSPTQDPQIVNLGFDAEADYHVYDIE
                     WTPSKIRYLVDGAVLREWTRQIELMKRPLTILLTIWASNSASWAGAIGPTSVPARASI
                     DWIRVYDWRG"
     gene            complement(6134..7243)
                     /locus_tag="VFQ61_13095"
     CDS             complement(6134..7243)
                     /locus_tag="VFQ61_13095"
                     /inference="COORDINATES: protein motif:HMM:NF043005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCE4755 family polysaccharide monooxygenase-like
                     protein"
                     /protein_id="HET9955442.1"
                     /translation="MNQGWSRTRALGAGAFIPSLGLTFAALLTVAPAGAHFKLEAPDA
                     WAEQDNLGNPQKAGPCGERDSGTTDTAKPTNKVTAYKAGDTITIKLTETVPHPGHYRV
                     ALAKTRAELPPDPKVTAGSTACGSAEIDMTPEFPVLVDGALQHTQNLSGQQTISVKLP
                     SDFSCENCTLQVIEFMSNHGLNNPGGCFYHHCANISVSATGDSGTGGAGTGGSGQGSG
                     GQGGSPQAGSTQGGASAAGATNGGAAAGGSMSSGGTLAQTGGASGSGGASATGAATSS
                     GGVSNTGGAAAPGGSSAGGAPAQGGASSAGNPSTGSAGTPASSAGNSGNPPSGGDDDG
                     GCSIGSLPSAGSSGLGLAFAAAALGFARRRRTRRG"
     gene            complement(7437..>9030)
                     /locus_tag="VFQ61_13100"
     CDS             complement(7437..>9030)
                     /locus_tag="VFQ61_13100"
                     /inference="COORDINATES: protein motif:HMM:NF014335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HET9955443.1"
                     /translation="QVLGNKTAQGNFMHRILQLSMLCGTGVLSVPALAANQDTQGPEE
                     LRLAQAPTNPVDSVPPAADRPAELAPAPAATQPSTAAAAGQATTGQTGTAQQTEVEQE
                     RTAAPTSPPLRPEVMVDAAAPPKPVVGDVSLHGYFRIGWGMSSEKGRQVCFQVAGAMS
                     KFRLGNECEQYGEFHFAAPMYVAKDGTVANVHFMPAVYIPTSRVGYPGAINSNAGETW
                     NGASWGFPFLYMDVQNMPGLSGGTAWVGRRYYKREDWNATDFFYWNPSGLGAGIEDIH
                     IGEELRFSYAAFTVDPPAVAPPVATPWLPSIVPQNSVGIRNDFQLRGIRLYPGGELQL
                     GANVVANWSDNPATHGGWGGLVRHVQDVANGNNKLAVQYGRGAGVGFGIPDAPTADSK
                     TSRLRIVDVLTIDPVDWFGAQAGFVYQHDKMTGGSQDWISAGLRSSFAFSEHGKVVLD
                     IGHDNVKPSDGPRRTLTKFTLAPTLSAGRGVMTRPELRLFCTVAMWNAAARAAGVDSE
                     GIYTNTDKKVGATFGVHSEVWW"
BASE COUNT         1595 a         2914 c         2819 g         1702 t
ORIGIN      
        1 tgatggcgta gttcacgacg gtgtcgacgc gtgccatcca cgtggcatcg atcgtaaagc
       61 ttgggcttgg gccgaggtgc tccgaccagg taacggggat gcgcaccacg ttgaatccca
      121 gtgacgtgac ataggtcagc atgccctggg tcaccatcgg gttgccccag caggtctccg
      181 caccggcggc tccgcagcca ttcgaatcga ggctatttcc caggttccag ccgagtttcc
      241 aggctgcgac gagatcggcc gccttggtcg cttggctaat caccgagtca ggcacgccac
      301 ctttcatcgt agctcccgaa ctgcctgcgg atcccggtgc ggagccaccc gagccttgcg
      361 tggagccgcc ggcaccagca gcagctccgg cggtgccacc gttcactccg ccgcccgtgt
      421 tggcggtcga accgcctgag ctcgaggcgc cgccgagccc ggcggagcca cccgagccgc
      481 cgctgagtcc cgcggagcca ccgctcgaga cggagccccc gctcgcgcct ggcgaaccgc
      541 ccgagcccga gccgccggtc gcgctcgaga gcccacctgt ggtgctcaga ccacccgaac
      601 tcgcgccgcc ctgagccgcg ctcgcgcccc ccgaactact tccgccggag ctggtggagc
      661 ctccgattcc aggcgatccg gctggacctg agccgccgct accttcgctc gggagaggcg
      721 tgccagtcga gctacacgcc gagagcaacc agaaaccaag accgaggagt ggagagcgca
      781 tgccgtaccg tgtcctagcc ctccctcagc ggctcagccc tgcgagttcc gccagagaaa
      841 cgagggcggt ggagtgaacg ttccagcgcg gtacgcccgc tccaccgagc aggacggacc
      901 ggccgcgacg aagtttgttc tgctcgcggt tcgcgaccgg gcgtagtccc tccaccactt
      961 tggcgtcact cactggtcct cacttcgcat tgaggctaac aaagaaatcg aagacgctgg
     1021 gcagcaccaa gctcaccatc ccgccatggt cggcgttgac ctccttgtac tcgactttga
     1081 agccgttctt gtcgagccaa gtacggagcg ctctgctccc ctccaaggaa ggggcttggg
     1141 tgccctcggt cacgaataga gaaattgggc ggacgctctc ccacgggtag cccgactcct
     1201 gtacgaatgg cccagacatc ggcgcgaggc cggcccaata cttcgcgtac ttgccgccga
     1261 tgtaccaagt gcctccgctg cccatcgagt ggcccgtaag gaacaccgcg cctcgattga
     1321 ttggatattc gttgagcacc aactcgagca cattgatgac gtctcgttcg ctgagctcat
     1381 tggtcttttc gctgtccgcg gtcacctggg ccatgagctt ggccgcttca tcgggttttc
     1441 cgaatggggc tgccaatctc aggaagttgc cgtacgcacc tttgtcaccg agtggcgcta
     1501 ccaagacgaa gccaccttgc tgcgccagat tgattagctg cttgttgttt tgatcgacgt
     1561 acgtgttttc gttggcgccc gagccgtgca gaaacatgac cagcgtcaac atggactgcc
     1621 cgtcccaggt cgtgggaacg tacaatcgat aaggcgcggt cgtgtcggcc tctgcaaaac
     1681 gataactccg gtgttgatct cccttcgaac gccattcggc ggcgtttgga ttggcctgcg
     1741 cttgggccag ggcttgggcg gctctgatga ccgcctcggc atcgagcaac gttccgaacg
     1801 agatcgatcg ctcattgctg gagcgcccac ctgtgctgga cattcctgca gatgcggtgc
     1861 gcccgccttc acccgtgacc ccgccggatt ggcttccggc agcgcccgcc ccagcttggc
     1921 cgccgtccgt cgctcgaccg ccactctcgg caccgactcg ccccgaagct ccgcctgctg
     1981 cagttccacc gataacgcct ggtcccgagc cgccgagtcc ggcggtgctc gcgcctgctc
     2041 cggcacctcc ggacatggtg ttggtgtcgg tgctcgtgct cattccgccg gaagggtcga
     2101 acacagcacc gacgcctccg ctcgtgcttg agccgccgac gccagacggc ccggtcgtag
     2161 cgccgcctcg ttgggttgcg ctcgtggctg agccgctact cgttccgccc ccggcctgtg
     2221 gcggggttgg tccttgatcc cctgctgatt ggctgcatcc gcatggggcg atactcagca
     2281 ttagaaataa gagagagtgg cggcgtaagc gtgcagtcat cacgtttcgg gctccggttc
     2341 cggggactaa ccccaatgcc ggcagtggct cgcatcgctt caaatcgggt caaaaacgag
     2401 atcatgcccg aggttaccgg gtgcgcaaaa tgtgcgcagt ggaaagttct gctcaccagg
     2461 aacaccgtgc gatgagtctc cgggttcgga gacgggctcc agttgacgct acctcgaacc
     2521 agagcgtgac atccatcaag gcagtgcgaa gcgataggca ctaaagcttc cccatgagct
     2581 cctcgatcca cgggcatgaa gttctcgaga tgatggtgac tgcgggtatc ccattcacct
     2641 ctgcgtcatt acgcgacgcg attattcaac gcttcggcgt cgaagcgcgt ttccacacct
     2701 gttctgcgcg cgacatggat gcagcggctc tcgtcgactt cttatccgcc cggggcaagt
     2761 tcgtgccagt ttcggatggc ttcgtgtttt cgggtaagtc ctgctctcac ggcgatgatg
     2821 cagaccatca ccattgattc tcgagcttac ttcagtgcgt cgagcgccaa tcgcgcatcg
     2881 acgtgggttc gcgcgcccgc gaagtaatct cgaggggcgc agcgcatgat gcgctgaaac
     2941 acacggtgac attgagcgta gctgccgaaa tgcgcctcga gcgcggcttg ggtaaagctc
     3001 tgtcccgcgt cgaccccgtc gatgaagtgc atcagacgct ggcgggcccg atactccacg
     3061 agcctgagcc cgagatcacg cttgaacaag cgactgactt ggctcgggtt gcttcgggcc
     3121 aacagcgcga gctcacgctc ggaggcgctt gggtctcggg tgagttcgcc catcgcacgt
     3181 cgactcacga cgtgggcctg gcgcaggcgc ggccccatgc gttcgaacac gtccgcgatc
     3241 aagccctcta ccgcattgcg atctcgcagc tcgctcgagg ccgcgagtcg attacccagc
     3301 tggttggtct cttgtgtgga gagcttcacc ggctggatcg cgtgagccga cggccagctc
     3361 gatatcttgg ccgcgagctc gggcctgagc gccaacacga agagatctag gtcgtcactc
     3421 gcgtactcga gctcatggtc ttgcccgggc tgaaaacata gcagtgcgcc aggccccgcc
     3481 tcgatccgca gttcgccaac cctgatgagg gcgtgtccgc gcagcaccag gttcagctca
     3541 ggctcgggat gaaaatggcg cggccgtcgg aaagccggct gatgccgcca cgcctgggcc
     3601 gcccgccctt cgagcatcgg aaaactttgg agcaacggtg cctcagcagc ggccacactc
     3661 gtggatagca tcgcggacga gccttgccga gggaaacacg gccgagggaa acggcccagc
     3721 gaaatacggc cgcacgcaat gctctaaagc accttggcgc tcaacaccgc ccgactaaca
     3781 cggccgagca aacgtgccga gcgagatccg ttcggccaaa gctcaaaaag tgagatgtta
     3841 ttcgagggcg ctggacgcat acgtcgcgca tgagaatggt gttgtgcgcc ggtttggctt
     3901 tggtgtgcgc agcatgcggg gcatccagcc cgtctgcggc acccgagcag gggggcgagc
     3961 cgccccagag cattggcacg tttcggctgc tgtatcgcga cgacttcgac actctcgatg
     4021 cctcgcgttg gcaggccatg acgcactcct gggatagcaa cctggcgctc ttctcctcgg
     4081 atagcgtcgc ggtgcaggac ggcaagttgc aattgagttt gctcccggca cccaccggaa
     4141 cgaaggactc gagcggagcg accaagtcct tcttgggagc cgaagtgcga tcgcgcgata
     4201 ctctgactta tggtcgggtg cgtgcgcgga tcaagttcgc ccgtggcccg gcggtgatct
     4261 cgtccctcgt cacgatctat acgccttggc ccgcggacaa ctggaacgag ctcgatatcg
     4321 agtgcctcgg gaaggcgccg caaagcttgc agttcaatac ccaagtctat ctcggcacac
     4381 tgccggcagc ctctacgccg gtgagcccga gccctgcgga aaagaaggtt gatttgggct
     4441 tcgacccaag cgaggatttt cacgattacg atatcgagtg gacgccttcg ggagcgcggt
     4501 tttcggtcga ttcgcgggtg ctgcacacgt ggacgcaaca catcgacctc atgaagctgc
     4561 cccagaacgt tctactcacg atttgggcct cctcgagcgc gggctgggca ggggccgtgg
     4621 gtcccgagac tagctcggcc aaggcctact acgattgggt cgagctgtac cggtacgagc
     4681 catgagcggg tggatgcctc gggcgcggcg ggtggcttgg tgcgcgccag tcttggtggc
     4741 gtgcaccagc gcgaatccga tgcagctcgg tggcgctggc gcaagctcga gcgcaggcgt
     4801 ggcgaatgcg agcggcggcg ctgggcaaag cgcaagctcg ggaggaggcg ctacggccgc
     4861 tggctctccg tcgggaggca ccggcgcggt cggcaccagc acgaccggca ccggcactgc
     4921 tgccggtggc gcgctaccga ccggcggcag ccgcggtgac gaacaacaca gtggcggcaa
     4981 ctcctcgagc ggcgctcaac aagggcaggg tggcgctcgg tcttccacta cggcggcggg
     5041 tgggatcgcg aacgcaagcg gtggtgccag ctcgagcggt ggacaagcaa gcagcgcagg
     5101 cacaccgaat ggcggagccg gagcaccgat gggcggtacc agcgcgagcg gcggctcttc
     5161 acaagctgga gcggctgcga atggaggcgc cagcgacccc ttcacgctgt cttggcaaga
     5221 cgagttcgag acgctcgata ctgcaaggtg gcagctccag aacttcacct gggaaggtaa
     5281 ccaagcgcaa ttcacgccgc aaaatgcggc cgtcgcgaac ggcatcctga cgctctcgtt
     5341 gaccaccgcc gaaagcggag ccgccaaacc gtacctcggc gtcgagatgc gctctgccaa
     5401 gacgctgctg cacggcaaag tgtctgcgcg catgcgcttc gccaaaggcc ccggtgtcgt
     5461 atccgggttg gtgctgttct acacgcctta tcccaattgc gactggaacg agatcgacat
     5521 cgagcacctg ggcaattcca gcaccacctc ccaactaaac gccatggtct tcacggggac
     5581 acccatggcg aactgcacga cgtccgtgtc gcccactcaa gatccgcaga tcgtcaatct
     5641 gggtttcgat gccgaggccg attatcacgt ttatgatatc gagtggactc cgagtaagat
     5701 tcgatatcta gtcgacggag cggtgcttag ggaatggact cggcagatcg agctcatgaa
     5761 gcgcccgctc acgattttgc tcacgatctg ggcttccaac tccgcttctt gggccggggc
     5821 catcgggcca acgtccgttc ccgctcgcgc cagcatcgat tggattcgag tgtacgactg
     5881 gcgcggctga gagctgctct ataactagaa tcatcggggc agtagcaccc aagcgttcgg
     5941 ccttctccga gggcctgcgc ggtgcacggg tgaacggagg ggactggggc tgcgctgtcg
     6001 cgcgcctgca tcgggtggcg cgcccgtata cgatgtggct cgagctgctg aggctctgaa
     6061 gaagaatgcc tggggtgaag gcgagtgcgg cggacgctcg cactcgcccg gttcactcga
     6121 gcgttctacg cgctcagcca cgtcgcgtgc gccggcggcg tgcaaaacct agcgcggcgg
     6181 ccgcaaaggc gaggccgagc ccactcgatc cggcgcttgg tagcgagccg atcgagcagc
     6241 caccgtcgtc gtccccaccg gaagggggat ttccggagtt gccggcgctc gatgctggcg
     6301 tgcccgcgct gcccgtactg ggattaccag cactcgacgc gccgccctgt gctggcgcac
     6361 ctcccgcact cgaaccaccc ggggccgcgg ctccgccagt gttggatact ccgccgctac
     6421 tggtggctgc gcccgtcgcg cttgcgccgc cgctcccgga cgcgccaccc gtttgggcca
     6481 gagtgccacc ggaactcatg ctgcctccgg cggcggcacc tccgttggtt gcccccgccg
     6541 ccgaagcgcc gccttgggtc gaaccagctt gcggcgagcc gccttgtccg ccgctgcctt
     6601 gtccgctgcc gccggtaccc gcgccgccgg tgccggagtc tccggtcgcc gagaccgaga
     6661 tgttggcgca gtggtggtaa aagcaacctc ctggattgtt gagtccgtga ttcgacatga
     6721 actcaatcac ctgcagcgtg caattctcgc agctgaagtc gctcggcagt ttaacgctga
     6781 tggtttgttg accactcagg ttttgggtgt gttgcagtgc tccatcgacc aacaccggaa
     6841 actccggcgt catgtcgatc tctgcgctcc cgcaggcggt cgatcccgcc gtgactttcg
     6901 gatctggggg tagctccgct cgtgttttgg ccagagctac ccggtaatgc ccgggatgag
     6961 gcaccgtctc ggtcagtttg atggtgatcg tgtcacctgc cttgtacgcg gtgaccttgt
     7021 tcgtgggctt ggctgtatcg gtggtaccgg agtcgcgctc tccacagggg cccgctttct
     7081 gcgggttgcc taagttgtct tgctccgccc aggcatccgg cgcttccaac ttgaaatgcg
     7141 cgcccgcggg agcgaccgtc aacagagccg cgaaagtgag cccgagagag ggaatgaacg
     7201 cgcccgcgcc gagcgcccga gtacgagacc agccttggtt catgatgcac gaaggttgca
     7261 ccatcgagcc acggtttgca ggcgcgtttt gcgcgaccag cctgattccg ggagccttct
     7321 agagtacgga ccttcgagag aagaaacttc taatgtgtcg gaaagagtgc tgatggcgcg
     7381 ccgcaatctt gtcacgtcga ccgccgaaat tatcggctcg cgttgcaatc actccatcac
     7441 caccaaacct ccgaatgtac gccgaatgtg gcgccgactt tcttatccgt attcgtgtag
     7501 atcccctccg aatcgacgcc ggcggcgcgg gcagcggcgt tccacatggc gaccgtgcaa
     7561 aataggcgta gctcgggtcg cgtcatgacg cctcttcccg cgctcagggt cggagcgaga
     7621 gtgaacttgg tgagcgttct acgtggaccg tcactcggct tgacgttgtc gtgaccaatg
     7681 tccagcacca ccttgccgtg ctccgagaac gcgaagctac tgcgaagacc cgcggagatc
     7741 caatcctggc tcccgccggt catcttgtcg tgctggtaga cgaagcctgc ctgcgccccg
     7801 aaccaatcga ccggatcgat cgtcagcacg tctacgatgc gtaaccgact cgtcttgcta
     7861 tcggccgtgg gcgcgtccgg aattccgaat ccgacgcccg cccctcgccc gtactgcacg
     7921 gccagcttgt tgtttccgtt cgcgacgtct tgcacgtgtc gcacgaggcc gccccatccg
     7981 ccgtgagtgg ctgggttatc actccagtta gcgacgacgt tggctccgag ctgaagctca
     8041 cccccggggt acaggcggat tccacggagc tgaaagtcgt ttcgaatgcc gactgaattc
     8101 tgcggcacta ttgacggcaa ccagggagta gcaacgggcg gtgcaaccgc gggcgggtct
     8161 accgtgaacg ccgcatagct gaaacgtagc tcctcaccaa tatggatgtc ctcgataccg
     8221 gcgccgaggc ccgaggggtt ccagtagaaa aagtcggtcg cgttccaatc ttcgcgtttg
     8281 tagtaccgcc gacccaccca cgcggtgccg cccgacagtc cgggcatgtt ctgcacatcc
     8341 atatacaaaa atgggaaacc ccagctggcg ccgttccagg tttctcccgc gttcgagttg
     8401 atggcccccg gataacccac tcgagacgta ggaatataga ccgccggcat gaagtgaacg
     8461 ttggctaccg tgccgtcttt cgccacgtac atcggcgcgg cgaaatggaa ctcaccgtac
     8521 tgctcgcact cgttgccgag gcggaacttg ctcatggcgc ctgcaacctg aaagcaaact
     8581 tgccggccct tctcgctgct cataccccag ccaatgcgga aatagccgtg gagcgagacg
     8641 tcccccacga caggcttggg cggagccgcg gcatccacca taacctcagg gcgcaacggc
     8701 ggagaggttg gcgcggcggt tcgctcctgt tcgacctccg tctgttgtgc cgtgccggtt
     8761 tgtcctgtgg tcgcttgtcc cgcggccgcc gctgtagacg gctgggtcgc ggccggagcc
     8821 ggcgcaagct ctgcgggtcg atccgccgct ggcggcactg aatcgaccgg gttggtgggt
     8881 gcctgagcaa gtcgcagctc ttccgggccc tgcgtgtcct gattcgcggc cagcgcgggc
     8941 acggataaca caccggtgcc gcacaacata gacagttgca gtattcgatg catgaaattc
     9001 ccttgtgctg ttttgttccc caggacttgg
//
LOCUS       DASTKU010000384        29217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_963440, whole genome shotgun sequence.
ACCESSION   DASTKU010000384 DASTKU010000000
VERSION     DASTKU010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 29217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29217
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963440"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VFQ61_13105"
     CDS             <1..955
                     /locus_tag="VFQ61_13105"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET9955444.1"
                     /translation="SSTEFYGKFTCEPLERGFGITIGNSLRRVLLASLQGAAISAVRM
                     DGALHEFTTIPDVVEDVTDIILNLKEVVLKVESPRTYTVRLEKEGPGVVTAGDIKVVD
                     GLSVLNPDHVVATLDKQGPLSMELTVSVGRGYVPAERNKSPTMAIGTIPIDALFSPIR
                     KVNYTVQNARVGQVTDYDKLVLEVWTNGAVRPVDAVAYAAKIMKEQLSIWINFEETEE
                     AAHPSPGGDEQPLNENLFRSVDELELSVRSANCLQNANITLIGELVQKTEQDMLKTKN
                     FGRKSLKEIKEILATMQLGLGMKFDSWPAMLERWKQQQQQG"
     gene            960..1475
                     /gene="rplQ"
                     /locus_tag="VFQ61_13110"
     CDS             960..1475
                     /gene="rplQ"
                     /locus_tag="VFQ61_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET9955445.1"
                     /translation="MRHKKAGRQFGRNTSHRRAMLRALTANLFAHERIETTDAKAKEL
                     RRVAERLITRAARLGAVAYTPHDELNAADRAKRLAAQRQVARFLRRFATVKKDGQDQK
                     IDLLEKVFVDFAIRFNGRPGGYTRIIKLGRRRGDNAAMSLIELVEGNPVPPRRPSQKK
                     AKETAEPAASE"
     gene            1662..2474
                     /locus_tag="VFQ61_13115"
     CDS             1662..2474
                     /locus_tag="VFQ61_13115"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9955446.1"
                     /translation="MSSSAHSSNPPNLGHVPGGELPAADLRLVAPECRYEVEDGRVVY
                     VPPADEPHGVKHAAVAAIVKAHARDGYEVAVDMLTRTSEMDDIAPDVSVFPLERHADG
                     GRQLEELALEIVNTQRLNDAGRKASKLIGRGVRRVFAIDVTRQRVFEWSTELGTWAIL
                     GDAAEIVDRTLAAPLPVAALVRAVLTDDAVARALLVKENAVLQAALAARHAQGQVKGK
                     ADALLTVLSARGLSPTADQRARILSCESEAELERWLRVALRCQSMDELFDAR"
     gene            complement(2495..4165)
                     /locus_tag="VFQ61_13120"
     CDS             complement(2495..4165)
                     /locus_tag="VFQ61_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955447.1"
                     /translation="MPPIPAAPRVSLDKPSQPQLAELDARLASLTSDDPATREAAARE
                     LLEVDAHWVPAIAFRLESVAERADKEAMKRLFLDARKAAREQPREDEADEKRDDKRAR
                     PDYLELLVTRKSAQNKAYSDLLAVVGMSRMLSQIGSVEAVRTLLTVYVRFGEFLRIHT
                     QRELERLGDKALPALIEARRHPAEKIAQWARRQLDMLGRAVPSEAVRVTDLQVLADVL
                     RAYGRNRDPDAVRIIVSFANSERAVVRDAARQAIGLMGEVSMWQLRDTYEAVVGKKPQ
                     RDWSWERTARELFGEFDRARNQAVLKSYDAGRLARAKGDLPAMRAAFDQVLTENPNFE
                     PKAELIGGYLEFVKRYAQEKPDEARVALARAARLTDDPAEKARLESQLTLLRAEHSLH
                     EGVADATLFERALELDSGNAQARSKLAELKAGKVVKENQRTRYVAAGSVLLAALAGII
                     ALLRKPRPDVPEEKETAGRALVQREDPRPAPTQPTPAPTQPTQTQQSTANSTHGTPEA
                     PTAAEPESMSSAPQSIASTPDSPTQAAPSTAPENPPVSGAPDDPDKTS"
     gene            complement(4344..4622)
                     /gene="yccX"
                     /locus_tag="VFQ61_13125"
     CDS             complement(4344..4622)
                     /gene="yccX"
                     /locus_tag="VFQ61_13125"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET9955448.1"
                     /translation="MALKQVQLFVRGRVQGVYFRASAQREARRLGLTGWVRNRPDGSI
                     EIVAEGEEVQIRELYGWAQKGPSAARVDRVETRWRGYTGEFPDFRIVD"
     gene            4750..6603
                     /locus_tag="VFQ61_13130"
     CDS             4750..6603
                     /locus_tag="VFQ61_13130"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9955449.1"
                     /translation="MSSEPEPLRLDEDSRTRELRDALRREERISAALREVGIALGSTL
                     DLDDLLELILEKLTVLVEADRATLYLLDESRHEMVSRLVVGGQVRSIRMKVGHGLAGT
                     VALTGRPIRVRDASRDPRFEGDWDLLTGYHTTSVLAAPLKNHLGRTIGVIQVLNKKTG
                     EEFSASDEAILMSLSTQAAVAIDNSRLFLSLVQKNKQLSDATDQLERRVRDLSLLFDL
                     ERATAHATNQDTLVRATLERAAPACDARGGALLLVAEETGDLVLHLYDREASDELSHM
                     GIAAGEGLLGSCMETSSELLLTEAASDARYSSRVEGRFPFTPAAFLGVPLEGEPGPIG
                     AIALFNPRSQRAFDEDDRALLRLVAANVSTAVRLFNASAAREREERLSAIGRLLSQVI
                     HDFKSPMTVISGSVQLMAEEENPRKRQEYTEEILKQFDVLTAMQREVLEFARGERSIF
                     ARRVYIRKFFADLARQIELEIGEKPVKLEVNVDSKLVARFDEGRLARAIHNLARNAVE
                     AMRDKGGVLSIRARKDKSDLVISVSDTGPGIPKEIEGRLFQSFVTANKAGGTGLGLAI
                     VKKIVDEHGGSVKVKSSPKGTRFVMRLPQAGPPHSAAKPGSARNRPPSRAG"
     gene            6621..7739
                     /locus_tag="VFQ61_13135"
     CDS             6621..7739
                     /locus_tag="VFQ61_13135"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET9955450.1"
                     /translation="MTASRRSLPMVDRGSLAQPLFESRKAELETCAYCPKLCRAACPV
                     SDAEWSESVTPWGKMSSVYVAARHAAVPDGASAELPWACTGCLACRERCDHRNPVRDT
                     LFEARAAYVEAGAAPAGSERVRLRFGARIEATTQRQRELLNSELSGASSAEQPDVDLL
                     LGCAYVNHAPEAVRAALRAARNLFGRVRVIEGCCGAPLDAAGAPEAARATREKLLGSL
                     SPGARRIALDAGCTYSLRQDGFVPFSRTALDALATWASFPSFRGERLRYHDSCSLGRG
                     LGEYEAPRALLRRALGGEPLEFPRRREFAACSGGGGLLPLTRPETARAIAKDRVLEHE
                     RSGGGKIVTACAQSLRSLKQAGADVIDLSEIIRSLSER"
     gene            7736..9304
                     /locus_tag="VFQ61_13140"
     CDS             7736..9304
                     /locus_tag="VFQ61_13140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9955451.1"
                     /translation="MKEHSAVRQSVVARVLLSYALVVLGSALVATWSVLALRSGANEA
                     ALMRSGYHPLALAVRDFAAQQDIYNSQLNHITTARNPADLRAWLSATLTLGRPKMFGK
                     LKAAIGAAFPDEDNREMLRVRRELLAEVGAIERWFAGDQEPLGRLLDAIARRDDDAAE
                     LLRDELVERGARSSSRLVRLETRVEHEVDQLLNQARGRERLALGLLIVFSGVSLLVGL
                     LMALYARRVLRPLAAVTERAKSVAGGDLEPHAPIVSGDEIGELSTTFEAMVSAIARAN
                     EQLVQNERLATIGKMAAHVTHEIRNPLSSIALNLELLEDELGAQGDEARGLLRAISGE
                     VERLNALSQQYLSFARQKSVGMFEEDLAELVREACDFVRREFAKSEVQLTFECESGIG
                     PVRLDEGQIKQALFNLLRNARDAMPHGGTVQVFVRSLGDAVEITIEDEGVGLDQKARE
                     RLFEPFFTTKRHGTGLGLPITRKVVEAHGGSIEFSDRSPRGTRVSLRLPRQSSPKRSE
                     PDLQLASAATPAAS"
     gene            9347..9973
                     /locus_tag="VFQ61_13145"
     CDS             9347..9973
                     /locus_tag="VFQ61_13145"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HET9955452.1"
                     /translation="MSEPINIVLATTNAGKVRELAQLFASSPVRLMSVKDVLGAALDV
                     DETGTTFEENARIKARAIARATQQLALADDSGLEVDALQGRPGVYSARYAGPGARDAD
                     NNEKLLRELAAVPSALRTARFRCVLALVEPVRFEEVISSGTCEGRITNAPRGQQGFGY
                     DPLFEVARFPGLTLAELDAERKNSISHRASAARALLGAWSKLLAPRAV"
     gene            complement(10021..10869)
                     /locus_tag="VFQ61_13150"
     CDS             complement(10021..10869)
                     /locus_tag="VFQ61_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955453.1"
                     /translation="MSQAPEGGPAYYFEPPSAEGAEPEETREPQNEPPRSGVGGQAGP
                     GGQPPYYGPRLVWEPPPPPVPRHTAPRTSFWLGGRVGWFVPFGNAFSKNVYRGSYTES
                     VGVRLRDYASSGPFFEVDVGARIGRHYTVFGLWERAELGIGNGDRAGLNQVYTNLGGQ
                     TGGDTDFWAAAVRISSDPDEVGLVSELGIGYRRARIGFEGGEHLELTQGTFEARIGVG
                     ADIRVNRRFSLSPMLTLGVGMFGQVDLVRRNGDAVDLLRKDYNSDGHGWFTLHLGGHF
                     DIAGRP"
     gene            10917..11054
                     /locus_tag="VFQ61_13155"
     CDS             10917..11054
                     /locus_tag="VFQ61_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955454.1"
                     /translation="MAEPQALRAQAAWVRAREARPRPVLPVSEVLVLLPVLALALIAR
                     S"
     gene            11232..11510
                     /locus_tag="VFQ61_13160"
     CDS             11232..11510
                     /locus_tag="VFQ61_13160"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HET9955455.1"
                     /translation="MQQHWKSLGGPGTLGLEIALSVALGLFGGQWLDKKFGTAPWLTW
                     IGLAYGLAAATRAIYRALKQSKRELEELDREEQRAREKFNDEKPSHKR"
     gene            11482..11859
                     /locus_tag="VFQ61_13165"
     CDS             11482..11859
                     /locus_tag="VFQ61_13165"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HET9955456.1"
                     /translation="MMKSQATKDEASIAPVLWAVAIVGVLLALASASLFGSRGFTSTL
                     IGAALAVANLWAMARLVRGLLGAGPGRAAWGPLGMVKIAALFLVLWIILKRGYADVLP
                     LALGYAALPVGIVLAQLRNGGDN"
     gene            11861..12637
                     /gene="atpB"
                     /locus_tag="VFQ61_13170"
     CDS             11861..12637
                     /gene="atpB"
                     /locus_tag="VFQ61_13170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET9955457.1"
                     /translation="MPEHSTWLTLVLAHMRETLDHNAHALGDSVLGHHAPTWQSFEPI
                     IAALFIFLLVILVAARVRSRLSRVDEAVIPEERLTLRTFMETFLSYFYDLAKSVMGPE
                     RAKRYFPVVGTAAMFVFFSNVMALIPGMPVATSHLNITLGSALVVFVLFNAYGLMAQG
                     SGYIKHLAGPKLWLAPLIFPLEVASLCIRPVTLAVRLMVNMAVDHVLLSLFLGLVALL
                     VPIPIMLLGCLVVVVQTLVFTLLTCIYIGLATEGHEEHAH"
     gene            12775..13101
                     /locus_tag="VFQ61_13175"
     CDS             12775..13101
                     /locus_tag="VFQ61_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HET9955458.1"
                     /translation="MSKKKLALATVALSTLVSSTAFAQDAAAQSGGSGAQAMAALAAG
                     IAIGLAVIGGGLGQGRAAAAALEGISRNPGAAPRIQTPMILGLALIESLVLFAFVIAF
                     QLQGKV"
     gene            complement(13192..14439)
                     /locus_tag="VFQ61_13180"
     CDS             complement(13192..14439)
                     /locus_tag="VFQ61_13180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9955459.1"
                     /translation="MLLGSARAAAEGQGEPAFLNAGAGQHLSSWQSVEILRDDEPLYQ
                     QPSSRAARRGAARVSAQLPLFGGQRGPGCEGLWLQVGPSAFVCESGVRLSKLAPLRAS
                     EREPSLTSGLPYRYFYVARDGSFGYRGLETAEEGVPDAQFQPGFALAITRVAQHPNGD
                     PFGLTQRGFWVPLRDLSEAHAIEFRGADWSDELAWVTHEAAPVFAEVSRSSEAQRELR
                     REPGVTLPRLTQLRVLERRAVGGTQYARIAQERWLLSAHVTQPRAAAPPEELRTGERW
                     LDVDLSQQTLVAYAGTLPVFATLISSGRGREGSDTATPRGVHRVWVKLRTSDMSNLDD
                     AKARENYALEAVPWVMFFEKGYGLHGTFWHSRFGEVRSHGCVNLTPRDAERLFHWTSP
                     RLLPGWTAVFPTPYEPGTLVRVR"
     gene            14736..15905
                     /locus_tag="VFQ61_13185"
     CDS             14736..15905
                     /locus_tag="VFQ61_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HET9955460.1"
                     /translation="MAKHEPWGPMAVHGSGRYTFFRRTVIGFYIVAILGLALLMVVHE
                     TGHLAAARAFGMRVLRFSIGFGPPLWRYQSKKSGTVYQVAPIPFVAYVQIDGMNPFEE
                     ADPDDKGSYANASLLGRIVTIFAGPLANYLFASVLFFVAFFVGGETVPTTTVELLDGP
                     AKAAQMQNGDTIVAIDGVAVTDWEQMRSLILARSGKALNVEVTRGAERKTFSVVPVVR
                     EGRAMIGVAPVYKHVSLGARESLIRSVESPAMVVAQLVTGVGRMLAGKEKPQLTGPVG
                     IVKEAKRAAERSFTEYFRLLAILSAYLGGFNLLPIPALDGARLGFLGYEAVTRRKPNA
                     KVEAQVHFVGLLMLLALIFVVSSKELFSHEEGPGGAAPARPANSATSPAAPKPKP"
     gene            complement(15944..16645)
                     /locus_tag="VFQ61_13190"
     CDS             complement(15944..16645)
                     /locus_tag="VFQ61_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955461.1"
                     /translation="MSRWAQRKFEVLIVLGSLVLFAFGIGGAMAAYPGGTHLDPRTHG
                     YDFTGNFWCDMLRDPALNGSPNSQGAGLAALSLWIIGAGLLPFWGILAESCSERGSKS
                     WLRPTIRVLGISGSFGLMLVALLPSDRFPIWHGLAVTIGGTSGVVAAAVAIVASVVNG
                     SGPRYAARLGALTLGAAVANLVQYARQIWFDASYVALLPALQKIASLCFFLWVATLSV
                     SALRSKRRLPPPVNS"
     gene            complement(16788..17468)
                     /locus_tag="VFQ61_13195"
     CDS             complement(16788..17468)
                     /locus_tag="VFQ61_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET9955462.1"
                     /translation="MIRIRNLSKSFAERLVLSDISAEVPAGSVIALVGASGSGKSTLL
                     RCLNALESFDRGQIEIAGHRLDARTGEHELNRLRAAVGMVFQEYHLFPHLSVLDNVAL
                     APRVVSKLERKQANRVAMGWLERVGLADRARARPQELSGGQRQRVALARALAQSVRVL
                     LLDEPTSALDPALRFEVGELLRELTRASSAADAPLTSVLVTHDLELAHKLADEVWELA
                     GGQLRLPS"
     gene            complement(17495..19081)
                     /locus_tag="VFQ61_13200"
     CDS             complement(17495..19081)
                     /locus_tag="VFQ61_13200"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET9955463.1"
                     /translation="MKIACLSLAATGLFLAGSSVARANAPSTPGPAGFASPASTAALA
                     GSRASKQSVLSPELRWGGDLQGGEPYVFEAEGAPDGIRGFEVDIARALARRLDRKAHF
                     VQTDWSKLVPALERRDFDIILNGLEDTPERRARLGLSRPYFVYGETLTVRRTSPLVGF
                     EGLAQRRVGTLSQTVAEKLLREHDAEVVLYEGQQEPYLDLLAGRVEAVLLDHPIAQRY
                     GCNVSGLTCVSPDLARGHYVIATLPTERGLLESVDRALEGMRRDGELRHILEQWKLWD
                     ERQAEPSTDESTAADLAGKARGNSAGEARRVPSLPHEEVSFESPRWSAHLRLFLEGAL
                     LTLLLSLASFALASPLGISMAIVRLYGGRVARSCASAYVELFRGTPLLLQLYVLYFGI
                     SDYVRLSPIFAAVLGLTLNYGAYEAEVHRGALLALPRGQLEAARALGFSRWQALRHVL
                     LPQSLRQALPAATNDFVALLKDSSQVSVITVVELTKRMTIVAVEVRDWMVPGLLCAAL
                     YFILGFPLTRLSRRLERRAA"
     gene            19164..19838
                     /locus_tag="VFQ61_13205"
     CDS             19164..19838
                     /locus_tag="VFQ61_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955464.1"
                     /translation="MFPTGTPRQTCFSRVGCCAASVLTGLALLACTPNTVDLGAGPRE
                     YVAADYDVVLRNWTRSAELLAVSQMDSVLNVTATYESWDFRWAYAVRYAEDYRLTVDQ
                     RHDLLARTLAETRDHHAFYVALYAQKWKWNDLTAKQPAWIVRLIDDEGTETAPSEIEV
                     VRRPGAIELTYFPYTTAWRSAFRVQFPKIRADGRPTISAGARWFGLRFAGAQGNQELI
                     WEVAQR"
     gene            complement(19868..22237)
                     /locus_tag="VFQ61_13210"
     CDS             complement(19868..22237)
                     /locus_tag="VFQ61_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9955465.1"
                     /translation="MMHTAPPVFPQALDDDNDDVGWALETAKVQWTRGAYADAIEWLH
                     RAIEAAIDSGRAIRARHLTQQTNALETALQDGWIPDVQEFASEGEAEDGAEPLDLDAY
                     EEISVDVDVESLPPHARHSDAMELDASDLIEDVEEEPVSVLPIHAVRPAPAGSNSPSL
                     GSPQRSARNSALPRPAAGRSSVPAPRPSSAHAQPAHRVATTLRGPAPNLTYGSKAPQS
                     QFHQQQAPQPRSQQQQPSQAQLRQAPQPQLGRSWRQEEELPTENAFDPSAIAEPISSM
                     TSRSLPAFPLEEDTNQTELPLPSFALPDEVEPELKTMPESELPPPIAGVPPLNQLEPA
                     ARRREHETTDIEELDPDSLEPETESDAHATIEPEPLTELEPISEIEAISELEQEQERV
                     SAVSPSEVELEHVEPEQTGELLHGAEPENSLEPQQEPETTLISEMLLLSEPPRDSEHT
                     IVQAGPPEEETAQELYAAAPLPDEQATHEELPRESEEPRSASEHGPVSTAPENDGPRA
                     SIHPATTTEPSPRGRFASELEPTLVRSNPTLPARVGDIELATIAGLEDLPEAAQQRLA
                     TGAWVTTLAPEEEVTHFGLALIVEGTAAVMSTVADASGAQLAARDLALSHGHLQDSVA
                     LRLVAQGSGARIATWSEQELEAVLRDCPWVYDDLKTSGDRLQALTGITVGPMGEQLDD
                     MLRAMVVERCSVKHYLPHEVIAEAGRPVPGMIVLGAGRIEVLEPSSERIEENLKPGEF
                     LFPQAILQASPAPATARAGQSGALVVFAERRVAHELLVSVPPLLGIFAQ"
     gene            complement(22265..25042)
                     /locus_tag="VFQ61_13215"
     CDS             complement(22265..25042)
                     /locus_tag="VFQ61_13215"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET9955466.1"
                     /translation="MCPMGVRLFSPRSLHGSESVGTSSLPYGRWREASALLLFAGALF
                     VTLALASFEVDPNDPQVHGSDLSGVTGACIARYLVQGFGAVAWLLPVELVFVGLPLLR
                     GRTHPQLGLRVAGDLVVAIVLAALGQVAVPDGLVFGRAPFAGNVGLLFGELMREAFST
                     AGSFLVGPTVVALILIGRSSFSFIAFAERLSLWMRRIFALLAGFAGRVADAFREAAEL
                     RFRERSARKERPPVPIERPDPDALVLLQLASDDTEFQRVERTGVPPLTVSEALKKRRR
                     SLTDEAETPTQAGPEVLRLAQTPAPDAAAQEDTAGTRPRRSRRQKPKLDEARNEDRAL
                     EEAEPTLSVDARGPEPQPPEAQETGAPASEVGEREPSRSRGKRSAEEPAPAESAPEPP
                     SPRSSEPEIVDTQHERAVERAEITVAPVRDDFRLPAFDLLEESPPNNGAVSEEKLREN
                     GVLLVKTLLDYGVQATVEAILPGPTVTLYEVSCEAGTKVSKVAALADDLALGLSRKVR
                     IIAPIPGKNRIGFELPNEERIPVSLRELVEDQRFERLAEKAPLPVVLGRDIVGKPFYA
                     DLASMPHVIVAGATGAGKSVGLNVMLSSLLFKRTPQELRLLMIDPKVVELAPFDGIPH
                     MLLPVVTDMKQAATALRWAVDEMERRYQLFANAGTKNISTYNLWVKRVQDGELPPPKP
                     GEVEALSPEGAVISVPAAKQGSDGTSLPGKLPFIVVVVDEFADLMMQQGKEVEASVAR
                     LAQKARAAGMHVVLATQRPSVDVITGMIKANFPTRIGFRVAQKVDSRTILDEQGAEHL
                     LGRGDMLVKLNGSTETYRVQCPFVSEEEVSRLTDYLRAQATPQYYDAILTAKDDDDAA
                     ESDEEPLDPRFDEACRIVADTQRCSTSWLQRKMTIGYNRAAKIVEMMEKRGMVGPPNG
                     ARDREVFLPPI"
     gene            25273..26955
                     /locus_tag="VFQ61_13220"
     CDS             25273..26955
                     /locus_tag="VFQ61_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955467.1"
                     /translation="MSPNTTGLYYEDCSLLRETPRREIERTEQIGMHLGAPMVRVLGA
                     NVLVLALALGGCRTSEDDVHRWANTVQGPRKLLAVLVHQKYPVELRVEAAATLISMKP
                     RSGRRIGIQGSEDQPGLMDALAQLPPAARNAIVSRLVPRLLAEMKKPPPVAQAGQPAP
                     ADPSVPYKDAAFSLLTHNSGSLLADASQNAALREALASWASTNFAERLDDSSQLFGVE
                     QMMRELRADGVRSLPTLIAPGASKIDRIAELIADFGDPTTRMAASQKLVQVAKEVDSE
                     AWINQKAPAVEAANRVSKITVTKERFRKQLEQYQEEELLRVFTSMKKVGGKPVADYLL
                     QFAQDKSRGEKRRAAAMAALQGNLDRNNPAHAEAVLTIAQAADTPDQLRDAAFARVGE
                     FPRQMILSKLYGLFRNENWKVRWVAAELVLKTIETSDLPEFFKQLGQVGKGDGFAITE
                     PLRYGALIANMKGPPVREIVDKYAGTQHPVPVRMTALGYYFEAGSAADLPKIQPYEKD
                     AAKAPTCREGAKECEWKCEVVSGGSRETKDISTVGDFVSYCIKPAMESRAKK"
     gene            27093..28010
                     /locus_tag="VFQ61_13225"
     CDS             27093..28010
                     /locus_tag="VFQ61_13225"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9955468.1"
                     /translation="MTDPNKKLSRNFQCREALWQKFEQMAKELECSVDYLINDAMKQY
                     ARQRGYASVATSLGPGATSMSSQAPGSTAPGSSPNAITAPPAAPAANMAGMNMAPPPP
                     INQGIHAPPPPTGNVPLPPPGASRAGRPTPPPRKMGPPGPPPPPVAAHAAGSPAVHAP
                     PPPPPVAAHAAPPPPPPPGVAPPPRRGPPPLAPTRTLPPLPGGSGSQRAPAAGGALSI
                     HYQGEVFPVNKERFIIGRGKQNSDLTIKDPNVSRQHAMIEFSNGQYYIVDMGSTNGVE
                     FNGQRVQRRAITEGDIFKVCDHELRFSYQ"
     gene            complement(28157..28831)
                     /locus_tag="VFQ61_13230"
     CDS             complement(28157..28831)
                     /locus_tag="VFQ61_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955469.1"
                     /translation="MPLTRNPSALRPPQSLPLSGGLSSTALRTALALCLAACAFASTR
                     RAAADTLLIDRPGAHPRYSFEIEPHLALGLVDPPGPGHGTGYGLGARATIPLIYNGFV
                     ATINNSIGVGFGLDFVHYGNVRGRCNRLDLMGNCQDSVSDVSVDALWLPVVMQWNFWL
                     SRNWSVFGEPGIAFRYESNVAGDKELHFEPLQLYLGGRLHFSDAMTLTMRVGYPTFTV
                     GLSFLL"
BASE COUNT         5207 a         9285 c         9442 g         5283 t
ORIGIN      
        1 ttccagcacg gagttctacg gtaagttcac ctgcgagccg ctcgagcgtg gcttcggtat
       61 cacgatcggc aattcgctcc gtcgcgtgtt gctcgcttcg ctgcagggtg cggcgattag
      121 cgcggtccgc atggacggcg ctctgcacga gttcaccacc atcccggacg tcgtcgaaga
      181 cgtcacggac atcatcctca acctgaaaga ggtggtgttg aaggtggagt cgccccgcac
      241 gtacaccgtt cgcctcgaga aggaaggccc gggcgtcgtg acggcaggcg acatcaaggt
      301 cgtcgacggc ctgagcgtcc tcaaccccga ccacgtcgtt gcgacgctgg acaagcaggg
      361 gccgctctcg atggagctca cggtcagcgt cgggcggggc tacgtgcccg ccgagcgcaa
      421 caagagcccg acgatggcga ttggaacgat cccaatcgac gcgcttttct caccgattcg
      481 caaggtgaac tacacggttc aaaatgcgcg cgtcggtcag gtcaccgact acgacaagct
      541 ggttctcgag gtctggacca acggcgcggt gcgcccggtc gacgcggtgg cttacgccgc
      601 gaagatcatg aaggaacagc tctcgatctg gatcaacttc gaagagaccg aggaggctgc
      661 gcatccgtcc cctggtggtg acgagcagcc gctcaacgag aatctgttcc gctcggtgga
      721 cgagctcgag ctcagcgtcc gctccgctaa ctgcttgcag aacgccaaca tcacgttgat
      781 tggcgagctc gtgcagaaga ccgagcaaga tatgctgaag accaagaact tcggccgcaa
      841 gtcgctcaag gaaatcaaag agatcctggc gaccatgcag cttggattgg gtatgaagtt
      901 cgatagctgg ccggcgatgc tcgaacgctg gaagcaacag cagcagcagg gctgagatca
      961 tgagacacaa gaaagcaggc cggcagtttg gtcgtaacac cagtcatcgg cgcgcgatgc
     1021 tgcgcgcgct gactgcgaat ctgttcgcgc acgagcgcat cgaaaccacg gacgccaaag
     1081 ccaaagagct gcggcgcgtg gcggagcgct tgatcacgcg ggcagcgcgg ttgggtgcgg
     1141 tggcgtacac cccccacgat gagttgaatg cagcggatcg cgcgaagcga ctcgccgcac
     1201 agcggcaggt ggcgcggttc ctccgccgtt tcgcgacggt gaagaaggat gggcaggatc
     1261 agaagatcga tctcctcgag aaggtcttcg tggactttgc cattcgtttc aacggtcgcc
     1321 ccggtggcta cacccgcatc atcaagctgg gtcgccgtcg tggagacaac gccgcgatgt
     1381 cgctcatcga gctggtcgag gggaacccgg ttcctccgcg ccgcccgagc cagaagaagg
     1441 cgaaagagac cgccgaaccg gccgcgtctg aatgacgtga gctgaggctc ggtttcacgg
     1501 cgaggcagcc aatcccatgc gggtcggctg cctcgtttcg tttgtggttc cgagccgcgg
     1561 cggtgtgcct gttgccggct gtgtggctcg ccacgggctg tggcaatgcc gggcggtcct
     1621 ggtctccagt ccttcccgtg gggccgtgct agtctagcgc cgtgtcgtcg tcggcccact
     1681 cttcgaaccc ccccaacttg ggacatgtgc cgggtgggga gctcccggct gcagatttgc
     1741 ggttggtagc accggagtgt cgctacgaag tggaagacgg gagggtcgtt tacgtgccgc
     1801 cagcggacga accgcacggg gtgaaacacg cggccgtggc cgcgatcgtg aaagcgcatg
     1861 cccgagatgg ctacgaggtg gcggtggata tgctgacccg cacctcggag atggacgaca
     1921 tcgcgcccga cgtcagtgtg ttcccgttgg agcgtcacgc agacggaggg cgtcaactcg
     1981 aggagttggc gctggagata gtgaacactc agcggctgaa cgatgcgggg cgcaaggctt
     2041 cgaagttgat cgggcgtggg gtacgtcggg tgtttgcaat cgacgtgaca cgccagcgcg
     2101 tgttcgagtg gtcaacggag ctcggtacct gggccatctt gggagacgct gccgagatcg
     2161 tcgatcgcac gttggccgcg ccgctgcccg tagcggcgct ggttcgagcg gttctaacgg
     2221 acgatgccgt ggcgcgcgcg ctcctggtga aagagaacgc tgtgctccag gctgcgttag
     2281 ccgctcgaca tgcccagggg caagtcaagg gcaaggccga cgcgctactc acggtattga
     2341 gcgcgcgtgg cctcagccct acggcggatc aacgtgctcg catcctgagt tgcgagagtg
     2401 aagcagagct cgaacgttgg ttgcgggtcg cgttgcgctg ccaatcaatg gacgagctgt
     2461 tcgacgctcg atagcgcgcg tcgaggtcgc ggccttaact ggtcttgtct gggtcgtcgg
     2521 gtgcgccgct caccggaggg ttttctggcg ccgtactcgg cgcagcctgg gttggcgagt
     2581 ctggcgtgga agcaatactt tggggcgcag acgacatcga ctcaggctcg gccgcggtcg
     2641 gagcctcggg ggtgccgtgg gttgaattcg cagtcgactg ctgtgtctgc gtcggctgcg
     2701 tgggtgcagg cgtgggctgc gtcggtgcag gtcgtgggtc ttcccgttga acgagcgcgc
     2761 ggccggcggt ctctttctct tcaggcacgt cgggtcgcgg tttgcgcaat agagcgatga
     2821 tgcctgccag ggccgccaag agcacgctgc cggccgcgac atagcgcgtc cgttggtttt
     2881 ccttgacgac cttacccgcc tttagctcgg ccaatttact gcgcgcctgc gcgttccctg
     2941 agtccagctc gagggcgcgc tcgaacaacg tcgcgtcagc caccccctcg tgcagactgt
     3001 gctccgcgcg cagcaacgtc agttggctct cgagtcgcgc tttttcagcc ggatcatcgg
     3061 tcagacgcgc cgcacgcgcc agcgcaactc gagcttcgtc gggtttctct tgcgcgtaac
     3121 gcttcacgaa ctcgaggtag cctccgatga gctccgcctt gggctcgaaa ttcgggtttt
     3181 cggtgagcac ctggtcgaac gcggcgcgca tggcgggcag gtcacccttg gcgcgtgcca
     3241 ggcgcccggc atcgtaggat ttcagaacgg cctgattgcg ggctcgatcg aactctccga
     3301 acagctctcg cgcggtgcgt tcccaagacc aatcgcgctg cggtttcttg ccgactacgg
     3361 cctcgtaggt gtctcgcagc tgccacatgg agacctctcc catcaagccg atggcttggc
     3421 gcgccgcatc tcgcacgacg gcacgttcgc tgttggcgaa cgagacgatg attcggaccg
     3481 cgtctggatc gcggttacgg ccgtaagcgc gcagcacgtc cgccagcacc tgcaagtccg
     3541 tcacccgcac ggcttcgctc ggcacggctc gacccagcat atccagctga cgtcgcgccc
     3601 actgcgcgat cttctccgcc ggatgtcgac gcgcctcgat cagcgcgggc agcgctttgt
     3661 cgcccagcct ctcgagctca cgctgggtat ggatgcgcag gaactcgccg aaccgcacgt
     3721 aaaccgtgag tagggtccgc accgcctcga cacttccaat ctgagacagc atgcggctca
     3781 tccccaccac cgccagcagg tcggaatagg ccttgttttg ggctgacttt cgcgtgacga
     3841 ggagctccag atagtctggg cgagcgcgct tatcatcgcg cttttcgtcc gcttcatcct
     3901 cgcgaggttg ctctcgggcg gccttgcggg catcgaggaa cagcctcttc atcgcctcct
     3961 tgtcagcgcg ctccgccacg ctctccaggc ggaacgcgat cgccggcacc cagtgcgcat
     4021 cgacctccag cagttcgcgc gccgctgcct cccgggtcgc gggatcgtca gacgtcaagc
     4081 tcgccaggcg tgcgtccagt tccgccagct ggggctggct gggcttgtct aggctgaccc
     4141 gcggcgccgc cggaataggc ggcaagcttt ctgcagaacc ggtcgtgtgg gctcgcgccc
     4201 gaggtgccgc tgacagtgcc gcggcgccga tgagccctgc cacgagccaa gcgatgacgc
     4261 cggtcaggag ccttcgcgag ccaacccgcc ggaacggcgc gcaccacggt cgtgcgaccg
     4321 tggttggccg cgcgcgggct cggctagtcc acgattctaa aatcgggaaa ctcgccagtg
     4381 taacctcgcc agcgggtctc tacgcgatcg acacgggccg cgctgggacc tttctgtgcc
     4441 cacccgtaaa gctcgcggat ttgcacctct tcaccttcgg caacgatctc aatcgagcca
     4501 tccggtcggt tgcgaaccca tccggtgaga cccaggcgcc gagcctcgcg ctgggctgaa
     4561 gcgcggaagt agacgccttg aactcgtccg cgcacgaaca gctgaacctg cttcagcgcc
     4621 atccggcggg ggtatttagc gggacactcg cccacaagca acctcgactc acgacgtatg
     4681 gcgactcggc cgtctggcca gggcgcgccc cggtggatca aggctacgtt ttcgtgatgg
     4741 gatatccggg tgagctccga gcccgagcct ctgcgcctgg atgaagattc gcgcacgcgc
     4801 gagcttcgcg acgctttgcg cagggaagag cggatttctg cggccctgcg cgaagtgggc
     4861 atagccctgg gcagcaccct ggacctggat gacctgctcg agctcatcct cgagaagctg
     4921 acggtcctgg tggaggccga tcgggccacg ctgtacctgc tggatgagag ccgccacgaa
     4981 atggtgagcc ggctcgtggt gggcggtcag gtccgctcga tccgaatgaa ggtgggccac
     5041 gggcttgccg gtaccgtggc gctcacggga cgtccgatcc gggtccgaga cgcgtcacgg
     5101 gatccgcggt tcgaggggga ctgggatctg ctcacgggtt accacacgac cagcgtactc
     5161 gcggccccgc tcaagaatca tctcgggcgg accatcggcg tgatccaagt tctcaacaag
     5221 aagacggggg aggagttcag cgcgtcggac gaggcgatct tgatgtccct cagcacccag
     5281 gccgccgtcg cgatcgacaa ctcgcgcttg ttcttgtcgc tggtgcaaaa gaacaagcag
     5341 ctctcggatg ccaccgacca gctcgagcgc cgggtgcgcg atctctcgtt gctcttcgat
     5401 ctggaacgag cgacggctca cgccaccaac caagacacgc tcgttcgcgc caccttggag
     5461 cgagccgccc ccgcttgtga cgcgcgaggc ggcgctctgc tcttggtcgc cgaagagacc
     5521 ggtgacctgg tgctgcatct ttacgatcgc gaagctagcg acgagctatc ccacatgggc
     5581 atcgcggcgg gcgagggcct gctcgggagc tgcatggaga ccagcagtga gctcttgctg
     5641 accgaggcgg cgtcggatgc gcgctacagc tctcgggtcg aggggcgatt cccgttcacg
     5701 ccggctgcgt ttttgggggt gcccttggag ggcgagcccg ggccgattgg agccattgcg
     5761 ctcttcaatc cgagatcgca gcgcgccttc gacgaagacg atcgtgctct gttacgcttg
     5821 gtggctgcca acgtctccac ggccgtgcgt ctcttcaacg cgagcgccgc tcgcgaacga
     5881 gaggagcgcc tatccgccat tggccgcctg ctctcgcagg taattcacga tttcaaatcc
     5941 ccgatgaccg taattagcgg ctccgtgcag ctgatggccg aggaggagaa cccccgcaag
     6001 cgtcaggagt ataccgagga gatcctgaag cagttcgacg tgctcaccgc catgcaacgc
     6061 gaagtcttgg agttcgcgcg cggcgagcgc agcatttttg cgaggcgcgt ctacattcga
     6121 aagttcttcg ccgatctggc gcggcagatc gagctcgaga tcggggaaaa accggtcaag
     6181 ctagaggtca acgtcgactc gaaactggtg gcgcgcttcg acgagggacg cttggcgcgt
     6241 gccattcaca atctggcgcg caacgccgtc gaagccatgc gtgacaaggg cggagtgctc
     6301 tcgatccgcg cgcgtaagga caagtcggac ctggtcatca gtgtctccga tactggcccc
     6361 gggatcccca aggaaattga gggtcgattg ttccagtctt tcgtgaccgc caacaaggct
     6421 ggaggcaccg gattgggctt ggctatcgtc aagaaaatcg tcgatgaaca cgggggatcc
     6481 gtcaaagtca aatcttcacc caaaggcacg cgcttcgtaa tgcgcttgcc ccaagcgggg
     6541 ccgccccact ccgcggccaa gccaggatca gcgcgcaatc gtccaccgag cagggctggt
     6601 tgatcgcgca aagagcttgc atgactgctt ctcgccgctc gctaccgatg gtcgatcgag
     6661 ggtcgctcgc tcagccattg ttcgaatcgc gcaaggcgga gctcgagacc tgcgcgtact
     6721 gccccaagct gtgccgcgcc gcatgtccgg tatctgacgc cgagtggagt gagagcgtca
     6781 cgccatgggg caagatgagc tccgtgtatg tcgcagcacg gcacgccgcc gtgcctgacg
     6841 gcgcgagcgc agagcttccc tgggcctgca ccggttgcct tgcgtgtcgc gagcgctgcg
     6901 atcaccgaaa tccagtgcgg gacacgttat tcgaggcgcg cgccgcgtat gtcgaagccg
     6961 gcgcggcccc cgcgggttcg gaacgggtgc ggcttcgatt cggcgcccga atcgaagcca
     7021 ctacgcaacg ccagcgcgag ctcttgaact ccgagttgtc gggcgcgtcc agcgccgagc
     7081 aaccggacgt cgatctgctg ctgggttgtg cctacgtgaa tcatgcgccg gaggccgtgc
     7141 gcgccgcttt gcgagcagcg cgcaacctgt tcgggcgagt gcgcgtgatc gagggttgct
     7201 gcggcgcgcc tctggacgcg gcgggggcgc cggaagcggc acgagccacg cgcgagaagt
     7261 tgctgggctc gctatcccca ggggcgcgtc gcatcgcgct cgacgccggt tgcacctatt
     7321 cccttcggca ggacggcttc gttccgttct ctcgcacggc cttggatgcg ctcgcgacat
     7381 gggcaagctt tcccagcttc agaggtgaga ggctccgcta tcatgactcg tgttcgctcg
     7441 gccgcggact cggggaatac gaagctccgc gtgccttgtt gaggcgggcc ttgggtggcg
     7501 aaccgctgga atttccgcgt cgacgtgagt tcgctgcgtg ttcgggtggc ggcggcctat
     7561 tgccgctgac ccgcccggag accgcgcgcg ccatcgcgaa agatcgcgtg ttggagcatg
     7621 aacgctcggg cggcggtaag atcgtcaccg catgcgccca gtctctcaga agcctcaagc
     7681 aggcgggggc cgacgtgatc gatctgagcg agataattcg cagcctcagc gaaagatgaa
     7741 agagcactcg gcggtgcggc agagcgtcgt tgcgcgggtg ttgctgtctt atgcgctggt
     7801 cgtgctcggt tcggcgttgg tcgcaacgtg gagcgtgttg gcgctgcgct ctggcgccaa
     7861 cgaggccgcc ctgatgcgga gcggctatca cccgctcgcc ttggcggtgc gcgatttcgc
     7921 ggcccagcag gacatctaca actcccaact caatcacatc accaccgcca ggaatcccgc
     7981 agatctaagg gcttggttgt ccgccacgct gacgctcggg cggcccaaga tgttcggcaa
     8041 gctcaaggcc gcaatcgggg ccgcgtttcc cgatgaagac aatcgggaga tgctgcgcgt
     8101 acggcgtgag ctgctcgcgg aggtgggcgc gatcgagcgt tggtttgcgg gtgaccaaga
     8161 gccgctcggg aggttgttgg acgcgatcgc gcggcgcgac gacgatgcgg cggagttgct
     8221 gcgggacgag ctggtcgagc gcggcgcgcg cagctcttct cgactggtcc gcctggagac
     8281 gcgcgtggag cacgaggtgg atcagctcct caatcaggcc cgcggccgcg agcggctggc
     8341 cttgggcttg ctcatcgtgt tttccggagt gagcctgctc gtcggtctgc tcatggcgct
     8401 ctacgcgcgc cgcgtgttgc gcccgcttgc cgcggtgacc gagcgcgcga agtcggtcgc
     8461 gggaggggac ttggagcccc acgcgcccat cgtctcgggt gatgaaatcg gtgagctctc
     8521 gaccacgttc gaggccatgg tctcggcgat cgcgcgcgcc aacgagcagc tcgtgcagaa
     8581 tgagcggctg gcgacgatcg gaaagatggc ggcccacgtg acacacgaga tccgtaaccc
     8641 cctctcctcc atcgcgctga atctcgagct gctggaggac gagctcggcg cgcaaggcga
     8701 tgaagcacgc ggcctgttgc gggcgatctc cggggaggtc gagcgcttga acgcgctgag
     8761 ccaacagtat ctgtccttcg cgcgccagaa atccgtgggt atgttcgagg aagatttggc
     8821 tgaactggtg cgcgaggctt gcgatttcgt gcgtcgcgaa ttcgcgaaga gcgaagtcca
     8881 actcactttc gagtgtgagt cgggcatcgg ccccgtgcgt ctcgacgagg ggcaaatcaa
     8941 gcaggccctc ttcaacctct tgcgcaacgc tcgggacgcc atgccgcatg gcggaaccgt
     9001 gcaagtcttc gtccgttcgt taggggatgc cgtagaaatc acgatcgagg acgaaggcgt
     9061 ggggctggac caaaaagcgc gcgagcggct gttcgagccg ttcttcacca ccaagcgcca
     9121 cggtaccggt ctcggtctgc ccatcacacg caaggtcgtc gaggcgcacg gcggcagcat
     9181 cgagttttcc gaccgctccc cgcgtggcac acgcgtgagc ttgcggttgc cgcggcagag
     9241 ctcgcccaag cggagcgagc ctgatttgca gctcgcttca gcggccactc ccgccgcgtc
     9301 ctgagcggcg ggggcgcggc gcggcgctcg tgatacgaag cagcacatgt ccgagcccat
     9361 caacatagtt ctggccacga ccaacgcggg caaagtgcga gagctcgcgc aactgttcgc
     9421 ttcatcaccg gtgcgattga tgagcgtcaa agacgtgctc ggtgccgctc tcgacgtcga
     9481 tgaaacgggt actaccttcg aagagaacgc gcgtatcaag gcgcgcgcca tcgcgcgggc
     9541 cactcagcaa ttggcgctcg ctgacgacag cggcttggag gtggacgcgc tgcagggacg
     9601 gccgggtgtc tactctgctc gctacgcggg gccgggtgca cgcgacgcgg acaacaacga
     9661 aaagctgttg cgcgaactcg cggcggtacc gagcgcgcta cgtacggcgc gtttccgctg
     9721 cgtcctcgcg ctggtcgagc cggtgcgctt cgaggaggtc atcagctccg gtacctgtga
     9781 agggcgcata acgaatgcgc cgcgcggaca gcaaggattt ggctacgatc ctctgttcga
     9841 ggtcgctcga ttcccagggc tcaccctcgc cgagctcgat gcagagcgaa agaactcaat
     9901 cagtcatcgc gccagcgccg cgcgcgcgct gctcggcgcg tggtccaagc tgcttgcgcc
     9961 gcgcgcggtc taatcgttcc agcgtacgct ccagagcgag ccgaccgccc cggaacctgg
    10021 ctatgggcga cccgcgatat cgaaatgccc gcccagatgg agcgtgaacc aaccatgccc
    10081 gtccgagttg tagtccttac gcagcaagtc gacggcgtcg ccgttgcgcc gcacgagatc
    10141 gacttggccg aacatgccga cccccaacgt gagcattgga gacaacgaga aacggcgatt
    10201 gactcgaatg tcggcgccaa cgccgatgcg tgcttcgaac gtgccttgcg tcagttcgag
    10261 gtgctcgcca ccctcgaagc ctatgcgcgc gcgccggtag ccgatgccga gctcgctcac
    10321 caaaccgact tcgtcgggat ccgagctgat acgcacggcc gcggcccaaa aatccgtgtc
    10381 gccgccggtc tgcccaccca agttcgtgta cacctggttg agcccggcgc gatcaccgtt
    10441 cccaatcccg agctctgcgc gctcccatag tccgaacacc gtatagtgac ggccgatgcg
    10501 ggctccgacg tccacctcga agaacggccc cgaggacgca tagtcgcgca ggcgaacgcc
    10561 gacgctctcc gtataagagc cgcggtacac attcttcgag aatgcattgc cgaacggcac
    10621 gaaccatcca acgcgtccac ccagccaaaa agaagtgcgt ggtgccgtgt gccgcggcac
    10681 cggcggcggt ggtggctccc aaaccaaacg cggtccgtag tagggcggct gccctcccgg
    10741 ccccgcctgg cccccgacgc ccgaacgcgg cggttcgttc tgcggctctc gagtttcttc
    10801 cggctcagcc ccctctgccg agggtggctc gaagtagtaa gcgggcccgc cttcgggagc
    10861 ttgcgacacc gcgggcggtg gaggcgccgc cgcgggcggt ggaggcgtcg gcgtcggtgg
    10921 ctgagccgca ggcgctgcgg gcgcaggcgg cttgggtgcg ggcgcgggag gcgcggccgc
    10981 gaccggtgct gccggtgtcg gaggtgttgg tccttttgcc ggtgctggcg ctggcgctga
    11041 ttgcgcgtag ctagccgatg cccataagcc gaaagcaaac gcgagaggcg cggtgcgcgt
    11101 catggcggaa cgatagcaaa agccctcggt gttgttccaa tcggaggcac tgcacgttgt
    11161 tcggccgcca gcggggcacg ttgaccaaac tcatcgcgtc gtggtaagcc gccgccgctc
    11221 ccgcgaggaa tgtgcagcag cattggaaaa gccttggcgg cccgggaact cttggcctcg
    11281 aaatcgcgct cagcgtggcg ctggggctct ttggcgggca gtggctcgac aagaagttcg
    11341 gcacggctcc ctggctgact tggattggac tcgcatacgg tcttgcagcc gcgacgcggg
    11401 ccatctatcg cgcgctcaag caatccaagc gcgagctcga ggagctggac cgcgaagaac
    11461 agcgagctcg ggaaaagttc aatgatgaaa agccaagcca caaaagatga agccagcatc
    11521 gcccccgtgc tgtgggcggt cgcgatcgtc ggcgtgctct tggcgttggc ctcggcgtcg
    11581 ttgtttggct cgcgtggatt caccagcacc ttgattggcg ccgcgctcgc ggtcgcgaat
    11641 ttgtgggcca tggctcgact ggtgcgcggt ctgttgggcg caggacccgg ccgtgccgca
    11701 tggggcccgc tcggcatggt gaagattgcg gccctcttcc tggtgctctg gatcatcttg
    11761 aagcgcggtt acgccgacgt gttgccgctc gcgctcggtt acgcggcgct cccggtcggc
    11821 atcgtgctcg cacagcttag aaacggcgga gacaactgaa atgcccgaac acagtacttg
    11881 gttgacgttg gtgctcgcgc acatgcgcga aactctcgat cacaacgcgc acgcgctcgg
    11941 tgactccgtg cttgggcatc acgcacccac ctggcagagc ttcgagccca tcattgcggc
    12001 gctcttcatc ttcttgctgg tgatcttggt cgcggcgcgc gtgcgctcgc ggctctcgcg
    12061 cgtggatgaa gcggtgattc cggaagagcg tctcacgctc cgcacgttca tggagacgtt
    12121 tctctcgtat ttctacgacc tcgcgaagag cgtgatgggg ccggagcgcg ccaaacgcta
    12181 cttcccggtc gtgggcaccg ccgccatgtt cgtattcttc tcgaacgtga tggcgctaat
    12241 cccggggatg ccggttgcta cgagccattt gaacatcacg ctgggatcgg cactggtcgt
    12301 gttcgtgctg ttcaacgcgt acggcttgat ggcgcagggc tccggctaca tcaaacactt
    12361 ggcaggcccc aagctgtggc tcgctcccct gattttcccg ctggaagtcg cgtcactctg
    12421 cattcggccg gtgaccctgg ctgttcgtct catggtcaac atggccgtgg atcacgtgct
    12481 cttgagtttg tttctggggc tcgtcgcgct gctggtgccg attccgatca tgctgctcgg
    12541 ttgtctggtg gtcgtggtcc agacgttggt attcacgctg ttgacctgca tctatatcgg
    12601 gctcgcaacc gagggccacg aagagcacgc ccactgagcc accaaaaacc ggtggcgagc
    12661 agagcaaaaa agcactaaag ccgagcccgc aagaaagctc tatggaggac ctgccgggct
    12721 ctcgctgaaa ctgaggtcct ttcttagccg cgcaatcata aggagacgaa gatcatgtcc
    12781 aagaagaagc tggcacttgc caccgtcgca ctgagcacgt tggtatcctc gacggcgttc
    12841 gctcaggatg ccgccgctca gtcgggaggc tcgggggcgc aggcgatggc cgcgctcgca
    12901 gccggcatcg cgatcgggct ggcagttatc ggtggcgggc tcggacaggg ccgtgcagct
    12961 gccgctgctc tcgaaggcat cagccgtaac cccggcgctg ccccccgcat ccagaccccg
    13021 atgattctgg gtctcgctct gatcgagtcg ctcgttctgt tcgcgttcgt gattgccttc
    13081 cagcttcaag gcaaggtctg agtcccggcg cggttttccg cgcgggctct cttgtcgaat
    13141 cgcgtcggat agccattgcg cggcggtgct gctcagcggt gccgccccag gctatctcac
    13201 ccgcacgagc gtgccgggct catagggcgt tgggaaaaca gccgtccagc ccggcagcaa
    13261 gcgagggctg gtccaatgaa acagccgctc tgcgtcgcgt ggggtgaggt ttacgcagcc
    13321 atggcttcgg acctcaccaa agcgcgagtg ccagaacgtg ccgtgtaacc cgtagccctt
    13381 ctcgaaaaac atgacccagg gcacggcttc gagcgcgtaa ttttcgcgcg ctttggcgtc
    13441 atccaggttg ctcatgtcgc tggtgcggag tttcacccaa acgcggtgaa ctccgcgagg
    13501 cgtcgcagtg tcgctgccct cacgtcctcg tccgctagag atgagcgttg cgaaaaccgg
    13561 cagcgtgccc gcgtacgcga ccagagtctg ttggctcaag tctacgtcga gccaacgctc
    13621 tcctgtgcgc agctcttcag gaggggcggc cgcacgtggc tgcgtcacgt gtgcagacaa
    13681 gagccagcgc tcttgtgcga ttcgagcgta ttgggtgccg ccgacggcgc gtcgctcgag
    13741 cactctgagt tgagtgagac gcggcagcgt cacgccgggt tctcgccgta gctctcgctg
    13801 cgcttccgat gagcgcgata cttcggcgaa gacaggcgct gcttcgtgcg tcacccaggc
    13861 caattcgtcc gaccaatctg cgccacgaaa ctcgatcgcg tgtgcttcgc tcaggtcacg
    13921 tagcggaacc cagaagccac gctgagtaag accgaagggg tctccgttgg gatgttgcgc
    13981 gacccgagtg atggcgagcg cgaaaccggg ttggaattgc gcgtcgggaa cgccctcttc
    14041 ggcggtttct aacccgcgat aaccgaagga gccatctcgc gcgacgtaga agtaacgata
    14101 gggcagcccc gacgtaagcg acggctcgcg ctcgctggct cgcagcgggg ccaacttgga
    14161 aagcctgacc cccgactcgc agacgaaggc gctggggcca acttgcaacc acaggccttc
    14221 acagcccggt cccctttgcc ctccgaagag cggaagttgc gccgacacgc gggccgcgcc
    14281 tcgacgcgcg gcacggctcg acggttgctg gtaaaggggc tcgtcatcac gcagaatctc
    14341 gacgctttgc caggagctca gatgctgacc cgcccctgcg ttcaggaacg cgggttcccc
    14401 ctgcccctcg gcggcggccc gcgcggagcc gagcaacaat cctattccga caacccaaga
    14461 gaccagcccg ttggctcctt ggcaggcccg cgcccccggc gcttgcccct cggctgacac
    14521 cgcagcccgg cccactgact cagccccacg atatcctgct tgtgcacgac gtgcagccgg
    14581 cagcggcccg agtgcgccgg cgcaactgac gagttttcct cgatttctcg agatgcaccc
    14641 gtgcggcatg cttggcgcct ttgtcatgag ggtgaggaga cgcgccttcc ccgttcgctc
    14701 gctcgaccga gctctagacg tggagcagcg tcggcatggc gaagcatgag ccatggggcc
    14761 ccatggcggt tcatggatcg ggacgctata cctttttccg gcgcactgtg atcggatttt
    14821 atatcgtcgc aattttgggg ctggctctac tcatggtcgt tcatgagacg gggcatctgg
    14881 cggcggcgcg tgctttcggc atgcgcgtat tgcggttcag catcggattc ggaccgcctc
    14941 tttggcgtta tcagtcgaag aagagcggca cggtctatca agtcgcgccg attcctttcg
    15001 ttgcctacgt gcaaatcgac ggcatgaacc cgttcgaaga ggccgatccg gacgacaagg
    15061 gcagctacgc gaatgcgtcc ttgttggggc ggatcgtcac catctttgcc gggccgctcg
    15121 cgaattacct ctttgcgtcg gtgttgttct tcgtcgcctt cttcgtcggc ggcgagaccg
    15181 ttcctaccac gacggtcgag ctcttggatg ggccggcgaa ggcggcccag atgcaaaatg
    15241 gggacaccat cgtggccatc gatggggtcg cggtgacgga ttgggagcag atgcgaagtc
    15301 tcatcctcgc ccgttcgggc aaggcgctga acgtcgaggt aacgcgcggt gccgagcgca
    15361 agaccttcag cgtagtaccg gtggttcgcg aaggccgcgc gatgatcggc gtggcgcccg
    15421 tgtacaagca cgtttcgctc ggtgcgcgtg aatccctgat tcgctcggta gagagccccg
    15481 ccatggtggt ggctcagctc gtgacgggcg tgggtcgaat gcttgcgggc aaagagaagc
    15541 cgcaactcac cggccccgtt ggcatcgtca aggaagccaa gcgcgcggcg gagcggagtt
    15601 ttacggagta tttccggctg ctcgcgatcc tgagtgctta cttgggcggc ttcaacctat
    15661 tgccgatccc cgcgttggac ggagctcgac tcggctttct aggttacgaa gccgtgacgc
    15721 ggcgcaagcc caacgcgaag gtagaggcgc aagttcactt cgtgggcttg ttgatgctgt
    15781 tggcgttgat cttcgtcgtc agctcgaaag agcttttctc gcacgaagag ggtccgggcg
    15841 gcgcagcccc ggctcgtccc gcgaactccg cgacttcacc cgcggcgccc aagccgaagc
    15901 cctgaggcgt tctcggccgg ctcgatgagc tgcctcgcct tggtcacgag ttgacgggcg
    15961 gaggcagccg acgtttgctg cgtaaggccg agacgctcaa ggttgccacc cacaagaaga
    16021 agcagagtga cgcgatcttc tggagcgcgg gcagcaacgc gacatagctc gcgtcgaacc
    16081 aaatctgtcg cgcatactgg acgaggttcg cgaccgccgc gccgagcgtg agcgcgccta
    16141 acctagcggc gtaacggggg ccgctcccgt tgacgacgct tgcgacaatc gcgacggcgg
    16201 ctgcgaccac gccggaagtt ccgccgatgg taaccgccag cccatgccaa atcgggaatc
    16261 gatccgaagg caggagcgcg accagcatca agccgaacga gcctgaaatg cccaagacgc
    16321 ggatggttgg tcgcagccaa gacttgctcc cgcgctcact acacgattct gccaggatgc
    16381 cccagaacgg cagcaagcct gcgccgatga tccacaagga cagcgcggcg agccccgctc
    16441 cctgcgagtt cggggacccg ttcaacgcgg gatcccgcag catatcgcac cagaaattgc
    16501 cggtgaaatc gtagccgtgg gtgcggggat cgaggtgtgt tccgccggga taggcggcca
    16561 tcgcgccacc gatcccgaag gcgaacaaaa ccagactacc gagaacgatc agcacctcga
    16621 atttgcgctg cgcccacctg ctcatggctg ctcgagttta acttggattt ggagggtggg
    16681 actttgaaag ccttgtcccg aaagtaggat ttgtgcggcg ggtggcggga tgcattccgg
    16741 catcccctcc ggcatgcact gccttgctgg attccacggc cgcgcaccta gctcggaagt
    16801 cgaagttgcc cgcccgccag ttcccagact tcgtccgcca gtttgtgcgc caactccagg
    16861 tcgtgtgtca ccaacaccga agtcagcggt gcgtccgcag cgctgctggc tcgggtgagc
    16921 tcacggagca gctcacccac ctcgaagcgt agggccggat cgagggcact cgtgggttcg
    16981 tccaacagca aaacccgtac gctttgtgcc aaggcgcggg ccaaggctac gcgctgtcgc
    17041 tggccacccg aaagctcctg cgggcgagct cgagcgcgat cggccaagcc gacgcgctcg
    17101 agccagccca tcgccacacg gttcgcctgt ttgcgttcga gcttggaaac cacgcgcggt
    17161 gccagcgcga cattgtcgag caccgaaaga tgcggaaaca ggtggtactc ttgaaagacc
    17221 attccaaccg ccgcgcgcag gcggttcaat tcgtgttctc cggtgcgagc atccagccgg
    17281 tgcccagcga tttcgatttg accacgatcg aagctctcga gcgcgttcag acaacgcaag
    17341 agggtgctct tcccggagcc cgacgcacca accaaagcaa tcaccgaacc cgcaggaact
    17401 tcggcgctga tgtccgacaa caccagccgt tctgcaaacg acttgctcag gtttcgaatg
    17461 cggatcatgg gcacgggtta gctcacgagg cggcctaggc ggctcgacgt tcgagtcttc
    17521 gggagagtcg ggtgagcgga aatcccaaaa tgaagtaaag cgccgcgcac agcagcccag
    17581 gcaccatcca gtccctcact tccacggcta cgatggtcat ccgcttggtc aactccacca
    17641 ccgtgatgac gctcacctgc gatgagtcct tgagcagcgc gacgaagtcg ttggtagcag
    17701 cgggcagcgc ctggcgcagc gactgcggga gcaaaacgtg gcgcaacgcc tgccacctcg
    17761 aaaacccgag agcgcgcgca gcttcgagtt gcccgcgcgg cagcgccaac aaggctccgc
    17821 ggtgcacttc ggcttcgtaa gctccgtagt tcaacgtgag cccgagcacg gccgcaaaga
    17881 tcgggctgag cctcacgtaa tcggagatgc cgaagtacaa cacatagagt tgcaggagca
    17941 gaggtgtgcc tcggaatagc tcgacgtaag cgctggcgca gctccgcgcc actcgcccac
    18001 catacaagcg aacgatggcc atcgagatgc caagcggtga cgcgagcgcg aaagacgcca
    18061 gcgagagcag tagcgtcaac agtgcgcctt cgagaaacag tctcaagtgc gcagaccagc
    18121 gaggtgactc gaaggagacc tcctcatgcg gcagagaggg aactcgtcgc gcctcgcccg
    18181 cagagttgcc cctggctttt ccagccaaat cagccgcggt gctctcgtcg gtgctgggtt
    18241 cggcctgccg ctcgtcccaa agcttccact gctccaagat gtggcgcagc tcaccatcgc
    18301 ggcgcatgcc ctcgagcgcg cgatctacgc tctccaacaa tcctcgttcg gtgggaagcg
    18361 tagcgatcac gtaatggccg cgcgccaaat ccggactgac gcacgttagc ccgctgacgt
    18421 tgcaaccata acgctgagca atcgggtgat cgagcagtac cgcctcgact ctgccggcga
    18481 gcaagtccag atatggttcc tgttggccct cgtacagcac cacctcggca tcgtgctcac
    18541 gcagcagctt ctcggcgacg gtttggctca acgtgccgac gcggcgctgc gcgagcccct
    18601 cgaacccgac gagtgggctc gtgcgacgca ccgtcaaagt ctcgccgtaa acgaagtacg
    18661 gtctggatag tccgagccgg gcccggcgct cgggcgtgtc ttccagccca ttcaggatga
    18721 tgtcgaagtc cctgcgctcg agcgctggaa cgagcttcga ccaatcggtt tggacgaagt
    18781 gggctttacg atccaagcgt cgcgccaagg ctcgtgcgat atccacttcg aagccgcgga
    18841 tgccgtctgg cgcgccctcc gcctcgaaaa catacggctc tcctccctgc aaatccccgc
    18901 cccaccgcag ctcgggcgaa agcacgcttt gcttcgaggc cctcgagccg gcaagcgctg
    18961 cagtgctggc agggctggcg aagccggcag ggccaggcgt gctgggtgcg tttgcgcgtg
    19021 caaccgaact gcccgcgagg aacaggccgg tggccgccaa cgacagacac gcgattttca
    19081 tcccagaagc cagcgcagcg taatccaaaa agccagcgaa ctactcgaac cttttcggaa
    19141 agcggtacta cactgggcgt cgaatgtttc cgaccggcac tccacgccag acttgcttct
    19201 cgagagtggg ttgctgcgcc gcgagcgttc tgacgggact cgcgctgctg gcctgcacgc
    19261 ccaacacggt ggacttgggc gcagggccgc gcgagtacgt cgccgcagat tacgatgtgg
    19321 tgttgcgcaa ctggacccgc tctgcggagc tactggccgt ctctcagatg gacagcgtgc
    19381 tcaatgtgac cgcaacctac gaatcgtggg actttcgttg ggcgtacgcg gttcgctatg
    19441 cggaagacta tcgcttgacc gtcgatcagc gccacgatct cttggcgcgc acgctcgcgg
    19501 agacgcgcga tcaccatgcg ttctatgtcg cgctctacgc tcaaaaatgg aagtggaacg
    19561 atctgaccgc caaacaaccc gcgtggattg tgaggctgat cgacgatgag ggtacggaga
    19621 ctgcccccag tgaaatcgaa gtggtccggc gccccggagc gatcgagctc acttatttcc
    19681 cgtacacgac ggcgtggcgc agcgcttttc gggttcagtt tccgaagatc cgtgctgatg
    19741 gtcgacccac catctctgcg ggcgcgcgtt ggttcgggct ccgtttcgcg ggcgcacagg
    19801 gcaatcaaga attgatttgg gaagtggcgc agcgttgatg cacgcgctac ggaacggtcc
    19861 aagcccacta ctgggcgaaa attccgagta ggggagggac gctgacgagc agttcgtggg
    19921 ccacacgccg ctccgcgaac acgacgagtg cgccactctg gcccgcgcgc gcggtggccg
    19981 gtgcgggcga cgcctgcagg atggcctgtg gaaacaggaa ctccccaggt ttcaaatttt
    20041 cctcgatccg ttcgctcgac ggctcgagaa cttcgatacg acccgctccc agcacgatca
    20101 ttcccggcac gggccgacct gcctccgcga ttacctcgtg ggggaggtag tgcttcacgc
    20161 tgcagcgctc cacgaccatg gcgcgcaaca tgtcatccag ctgctcaccc atcggtccta
    20221 cggtgatgcc agtaagcgcc tgaagccggt cgccggaagt cttgagatca tcgtagaccc
    20281 acgggcaatc gcgcagaacg gcctcgagct cctgctcgct ccaagtcgca atgcgtgcgc
    20341 ccgaaccttg ggccaccagg cgcaaagcaa ccgagtcctg caggtggccg tggctgagcg
    20401 ccaaatcgcg agcggcgagc tgcgcgcccg aagcatctgc gaccgtggac atcacggctg
    20461 cggtcccttc gacgatcaaa gccaagccga agtgagtcac ctcttcttcc ggcgccaacg
    20521 tcgtaaccca agccccggtc gcgagccgtt gctgcgccgc ttccggtagg tcctcgagcc
    20581 ccgcgatggt cgcgagctcg atgtcgccca cgcgcgccgg cagagtcgga ttggagcgaa
    20641 ccagcgtggg ttcgagctcg gacgcgaagc gcccgcgcgg tgaaggctcg gtcgtggtcg
    20701 ccgggtgaat gctcgcgcgt gggccgtcgt tttcaggggc cgtcgagacg gggccatgct
    20761 cggaagcgga gcgcggttct tccgactcgc gcgggagttc ctcgtgcgtg gcctgctcat
    20821 ccggcagtgg cgcggccgcg taaagctcct gcgcggtctc ctcttcgggg ggccccgctt
    20881 gcacgatcgt gtgttccgag tctcgcggcg gctccgaaag cagcagcatc tcggaaatca
    20941 gggtcgtctc cggttcctgc tgcggctcga gcgagttctc cggctctgct ccgtggagca
    21001 gttctccggt ctgctctggt tcgacgtgct cgagttcgac ctcgctcgga gagactgcgg
    21061 aaacgcgctc ttgctcttgc tctagctctg aaattgcttc gatttcagag atcggttcga
    21121 gctccgtcag cggctcgggc tcgatcgtgg catgcgcatc actctccgtc tccggttcga
    21181 gtgaatcggg atccagctcc tcgatgtccg tcgtctcgtg ctctcgtcga cgcgcggcgg
    21241 gctcgagttg attcagggga ggcacaccgg cgatgggcgg cggcagctcc gattcgggca
    21301 tcgtcttcag ctcgggctcg acctcgtcgg gtagagcgaa gctggggagc gggagctccg
    21361 tttggttcgt gtcctcttcg agcggaaaag ccggcagaga ccggctcgtc atgctcgaga
    21421 tcggctccgc aatcgccgac ggatcgaacg cgttctccgt gggcagctct tcctcctggc
    21481 gccagctgcg tccgagctgc ggttgagggg cttgcctcag ctgtgcctgg ctaggttgtt
    21541 gttgttgcga ccgcggctgt ggcgcctgct gttggtgaaa ctgtgactgg ggcgccttgc
    21601 tgccataagt caggttgggc gctggaccac gcagcgtggt cgcaacgcga tgcgcgggtt
    21661 gcgcatgcgc gctcgatggt cgcggcgcag ggaccgagct acggccggcg gccgggcggg
    21721 gcaacgcact attgcgcgcc gagcgttgcg gcgatcccag tgacggagaa ttcgaaccag
    21781 ccggggccgg ccgcacggcg tgaattggga gaaccgagac gggctcctct tcgacgtcct
    21841 cgatcagatc cgaagcatcc agctccattg cgtccgagtg ccgcgcgtgc ggcggcaagg
    21901 actcgacatc gacatcgacc gagatctcct cgtaggcatc gaggtccagg ggctctgcgc
    21961 catcctcggc ttctccttcc gaggcgaact cctgcacatc cggaatccaa ccgtcttgta
    22021 gcgccgtctc gagcgcgttc gtttgctgcg tcaggtgccg agcgcgaatc gcgcggccgc
    22081 tatcgatggc cgcctcgatt gctcgatgaa gccattcgat tgcatctgca tatgcccccc
    22141 gggtccattg gaccttggcc gtctcgagcg cccaccccac gtcatcgttg tcgtcgtcga
    22201 gcgcctgcgg gaaaacgggc ggcgcagtat gcatcatgcc gaaactccct catagcacgt
    22261 gccttcatat gggaggaagg aacacttcac ggtctcgagc gccgttcggt ggtccgacca
    22321 tcccgcgctt ctccatcatt tcgacgatct tagctgcgcg attgtagccg atcgtcatct
    22381 ttcgttgcag ccaagaggta gagcagcgct gcgtgtctgc cacgatccgg caggcctcgt
    22441 cgaatcgcgg atcgagcggc tcctcgtcgg attctgccgc gtcatcatca tccttcgcgg
    22501 tcagaatggc gtcgtagtat tgcggtgtag cttgggcgcg taggtaatcc gtcaaccggc
    22561 tcacctcttc ctcgctgacg aaagggcact gaacgcgata cgtttccgtc gagccgttga
    22621 gcttcacgag catgtcgccg cgccccagca ggtgctcggc gccttgttcg tcgaggatag
    22681 tccggctgtc gactttctgc gcgactcgaa agccgatgcg ggtcgggaag ttcgctttga
    22741 tcatgcccgt gatcacgtcg acgctcggcc gctgcgtcgc gagcacgacg tgcattccgg
    22801 cggcgcgggc cttttgggcc agacgcgcca cggaggcttc cacttccttg ccctgctgca
    22861 tcatcaaatc tgcgaactca tccaccacca ccacgataaa gggcagtttg cccggcaacg
    22921 acgtgccgtc actgccctgc ttcgcggcgg gtacgctgat gacggctccc tccggtgaga
    22981 gcgcctccac ttcgcccggc ttcgggggcg gcagctctcc gtcctgtacg cgcttgaccc
    23041 agagattgta ggtcgagatg ttcttggtgc cggcgttcgc gaacagctgg taacggcgct
    23101 ccatctcgtc cacggcccag cgcagagcgg tggcggcctg cttcatgtcc gtcacgaccg
    23161 gtaagagcat gtggggtatt ccgtcgaacg gcgcgagctc gacgaccttg ggatcgatca
    23221 tgagcaggcg cagttcttgc ggggtgcgct tgaagagcag gctcgaaagc atgacgttca
    23281 agcccacgct cttgcccgcg ccggtcgcac ccgccacgat gacgtgcggc atcgaggcca
    23341 ggtcggcgta gaacggtttg cctacgatgt ctcggccgag caccaccggt agcggcgcct
    23401 tctctgccag tcgttcgaag cgttgatcct cgacgagctc gcgtaggctg accgggattc
    23461 gttcctcgtt cggcagctcg aacccgatcc gattctttcc gggaatcggc gcgatgatgc
    23521 ggaccttccg cgacaagccc aacgccaaat cgtcggctag cgccgccact ttgctgacct
    23581 tggtgccagc ctcgcaggat acctcgtaga gcgtgacggt ggggcccggc aagatcgcct
    23641 ccaccgtggc ttgcactccg taatccagca gcgtcttcac caacagtacg ccgttttctc
    23701 gcagcttctc ctcgctcacg gcgccgttgt tcggagggct ctcttcgagc aaatcgaagg
    23761 ccggcaaacg gaagtcgtcc cgtactggcg caaccgtaat ttcggcgcgc tctacggcac
    23821 gctcgtgttg tgtgtccaca atctcgggtt ctgagctgcg cggcgagggt ggctccggag
    23881 cgctctcggc gggcgcgggt tcttcagcgc tgcgcttgcc tcgggatcga cttggctcgc
    23941 gttctccgac ctccgacgcg ggggcgccgg tctcttgtgc ttctggcggc tgtggctctg
    24001 gtccacgcgc gtccaccgat agcgtgggtt cggcctcctc gagcgcgcgg tcctcgttgc
    24061 gggcttcgtc gagctttggt ttttgtcgac gcgaacggcg cggtcgcgtg cccgccgtgt
    24121 cctcttgcgc ggccgcatcg ggcgctggcg tttgagccaa ccgcagcacc tcggggccag
    24181 cctgagttgg cgtttctgcc tcgtccgtca gggaacgtcg acgcttcttc aatgcttctg
    24241 aaacggtcag cggcggtacc ccggtgcgct cgacccgctg aaactcagtg tcatcgctag
    24301 cgagctgcag cagcacgagg gcgtccggat caggccgctc gattggtacc gggggccgtt
    24361 ccttgcgggc gctacgctcg cgaaagcgca gctccgccgc ctcgcgaaag gcatctgcta
    24421 cgcgccccgc aaagcccgcg agcaacgcaa atatccgccg catccagagc gacagacgtt
    24481 ccgcgaaagc gatgaaggag aagctgctcc gaccgattaa gatcagcgcc accacggtcg
    24541 gccccaccaa gaacgagcct gccgtcgaaa atgcttcgcg catcagctct ccgaatagga
    24601 ggccgacatt tcccgcgaag ggcgcgcgac cgaacaccag accatcggga accgcaacct
    24661 gccccagtgc ggccagcacg atcgcgacga cgagatctcc tgccacccgt aacccgagct
    24721 gcggatgcgt gcggccgcgc aacagcggca atccgacgaa taccagctcg accggcagca
    24781 accaggcgac ggccccaaat ccctgaacca agtagcgcgc gatacacgcg ccggtgacac
    24841 cgctcagatc gctaccgtga acctgcgggt cgttcggatc cacttcgaag ctcgctagcg
    24901 cgagcgtgac gaacaacgcg ccagcgaaca gcagcaacgc tgaagcttct cgccagcggc
    24961 cgtatggaag cgaactggta cccacactct cggagccgtg aagcgatcga gggctgaaca
    25021 gacggacgcc cattgggcac atacacttac atgaacctac ctatgcgtat caagtttcgt
    25081 tggttggggg cattaagccg cgccgccgga tggcgccgaa tcgctgagaa aacgcgctct
    25141 gctggggtgg aggagggcag tgttgaccga gccagcccgc cgtctcccgc ggtggatcaa
    25201 acgatagagt ggagagccgg ccgaggtgtg tcgtggcaaa gcaccctgcc tgagacaaca
    25261 gcgttcgccc gcttgagtcc gaatacgacc gggctgtact atgaagactg ctcgctcctt
    25321 cgcgaaaccc cgcgtcgcga gatcgagaga acggagcaga ttggtatgca cctgggggct
    25381 cccatggttc gggtgctcgg cgcgaacgtt ctagtcctag cgctggcact cggcggatgc
    25441 cgaacgagtg aagatgacgt gcatcgctgg gccaacacgg ttcaggggcc acgcaaattg
    25501 ctagcggtgc tggtgcacca aaaatatccg gtcgagctca gggtcgaagc cgcagcgacg
    25561 ctcatcagca tgaagccgcg gagcgggcgc cggattggaa tccagggctc tgaagatcag
    25621 ccgggactaa tggatgcctt ggcgcagctg cctcctgccg cgcgtaacgc gatcgtcagc
    25681 cgattggtgc cgaggctgct agccgagatg aagaagccgc cgcctgtcgc gcaagccggt
    25741 caacccgcgc ctgcggatcc ttcggtgcct tacaaagacg cggcgttctc gttgctgacg
    25801 cacaactccg ggtcgttact cgcggacgca tcgcagaatg cagctttgcg tgaggcgctg
    25861 gcgagctggg caagcacgaa cttcgccgag cgtctcgacg actcgtccca gctgtttggc
    25921 gtagagcaaa tgatgcgtga gcttcgtgcg gacggggttc ggtctctgcc gaccttgatc
    25981 gcgccgggcg cgagcaagat cgatcgcatc gcggagctga tcgctgattt tggcgatccg
    26041 actacgcgca tggccgcgag tcagaagctc gtgcaggtgg cgaaggaagt cgactcggag
    26101 gcctggatca accagaaggc gcccgccgtg gaggcggcca atcgggtctc caaaatcacg
    26161 gtcaccaagg agcgcttccg taagcagctc gagcaatacc aagaggaaga gctgctgcgc
    26221 gtgttcacct cgatgaagaa ggtgggtggc aagccggttg cggattactt gcttcagttc
    26281 gcgcaagaca agagtcgggg agagaagcgg cgcgcggcag cgatggccgc gctgcagggc
    26341 aacctcgacc ggaacaaccc tgcccatgcc gaagccgtgc tgaccatcgc tcaagccgcc
    26401 gatacacccg accagcttcg ggatgcggcg ttcgcgcgag tgggcgagtt cccgcggcag
    26461 atgattctga gcaagctgta cggcttattc aggaacgaaa actggaaagt acgctgggtc
    26521 gcggccgagc tcgtcctgaa gaccatcgaa accagcgatt tgcccgagtt cttcaagcag
    26581 ctcggccaag tgggcaaagg cgacggattc gccatcaccg agccactccg atacggtgcg
    26641 ctgattgcga acatgaaagg cccgcctgtc cgcgagatcg tggataagta cgctggcact
    26701 cagcaccccg tgccagtacg catgaccgcg cttggttact atttcgaagc tgggtcggcc
    26761 gcggatctac ccaagattca gccttacgaa aaagacgcag ccaaggcacc cacgtgccgc
    26821 gaaggggcca aggaatgcga gtggaaatgc gaggtcgtga gcggcggctc tcgcgaaacc
    26881 aaggacatct ctaccgtggg tgatttcgtt tcgtattgca tcaagcccgc gatggagagt
    26941 cgcgcaaaga agtgaaagag cccgagcagt cggcggagac tgaagtagaa tggcgcccct
    27001 ccggctcccg acagccggtc gagccaaccc gacgctaacg gaacaagcca ccttgaaacg
    27061 tttcgcgacg taacgcgcga gcgggacccg tgatgaccga tccgaacaag aaactctctc
    27121 ggaattttca atgccgcgag gcgctctggc aaaagttcga gcagatggcg aaagagctcg
    27181 agtgctcggt cgactatcta attaacgacg cgatgaagca gtacgcccgc cagcgcgggt
    27241 acgcttcggt ggcaacctcg ctgggccccg gcgccactag catgtcgagt caagcgcctg
    27301 gatcgacggc gcccggatcg tcgcccaatg cgatcacggc accgcctgca gcgccggccg
    27361 cgaacatggc ggggatgaat atggcgccgc cgcctccgat caatcagggc attcacgctc
    27421 cgcctccgcc gaccggaaat gttccgttgc cgcctccggg tgctagtcgg gcagggcgtc
    27481 caacgccgcc tccgcgcaag atgggccccc ccggcccgcc gcctcccccg gttgcggccc
    27541 atgccgccgg ctcgcccgcc gtgcatgcac cgccgcctcc tcctccggtc gcggctcacg
    27601 cggcgcctcc gccgcctcct cctcccggtg tcgcccctcc gcctcgacgc gggccgccgc
    27661 cactcgctcc gaccagaacc ctgcctccac tgcccggtgg gtcgggttca cagagggctc
    27721 ccgccgcggg cggcgcgcta tctatccact accagggtga agtgttcccc gtgaacaagg
    27781 agcgcttcat catcggtcga ggcaagcaga acagcgacct aacgatcaaa gatcccaatg
    27841 tatcccggca gcacgccatg atcgagttct cgaacgggca gtactacatc gtggatatgg
    27901 gttcgaccaa cggcgtggaa ttcaacggtc agcgcgtgca acgtcgcgcc atcaccgagg
    27961 gcgacatctt caaggtctgc gatcacgaac tgcgcttctc gtaccagtga gtgaggcgcg
    28021 ccgacgccgg acgaggaacc tgccaaatgg acctcgtccc agcgcttcgg cgagcacctc
    28081 cgagagcagt tccgagagca gttccgagat cacactgccc agaaaccacc tccaaatcgg
    28141 ccgaaagcag gatgctctag agcaggaaag acaacccgac cgtgaacgtc gggtatccca
    28201 cccgcatcgt cagggtcatt gcgtcgctga agtgcaggcg gccgcccagg taaagctgaa
    28261 gcggttcgaa atgcagctcc ttgtcgcccg cgacgttgga ctcatagcga aacgcaatcc
    28321 ctggttcgcc aaagacggac cagttgcgac tcagccagaa gttccactgc atgacgaccg
    28381 gaagccaaag cgcatccacc gatacgtcag acacggaatc ctgacagttg cccatcagat
    28441 cgaggcggtt gcagcgccct cgcacgttgc cgtagtgcac gaagtcgagg ccgaacccaa
    28501 cgccgatgct gttgttgatg gtcgcgacga acccgttgta aatcagggga atcgtcgcgc
    28561 gagctcccag gccgtagccc gtgccatgcc ccggtccagg gggatcgacg agccccagag
    28621 ccaggtgcgg ctcgatctcg aaactgtaac gcggatgagc gccggggcgg tcgatgagca
    28681 acgtgtcggc cgcggctcga cgagtcgacg caaaggcgca ggccgcaagg catagcgcga
    28741 gcgccgtgcg taaggcggtc gaactcaaac ctccagagag cggaaggctt tggggcgggc
    28801 gcagtgcact ggggtttcgg gtgagtggca ttgcagcggc ggaaagcatg cgcgacgcgg
    28861 cggtatgctg gcaagtgtgc atcccacatc cacgccgcgc cggcttcagt cttcgtgatg
    28921 cggccgcgga ggttggaacg ccccatccca gcgatcagct gtcctgaagc ccgggggagg
    28981 cccctattgc ggggccgagg cgtcgctgct actacggcca cgcgctgctc ttccgctccg
    29041 agggcctcgg tgtgcgagag ggggtgcagt ctcgcgcagc acagtcgctg ctgcgccgtt
    29101 cgaggtaatc gccgatgagt cagcccaatc cgcccagtgc accaggcaac ggacagccca
    29161 gtcttcaact ccaaggcggc gccgccatcg tggcgcccgt gagcgctttc cccggcg
//
LOCUS       DASTKU010000385         4213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_72865,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000385 DASTKU010000000
VERSION     DASTKU010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72865"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VFQ61_13235"
     CDS             complement(<1..972)
                     /locus_tag="VFQ61_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955470.1"
                     /translation="MHLRASLGTGLLVLAWGVSACGGGANKPATTPLNVESAPAATAA
                     PQPDLSPVAQPPDLVAVARVARAQSFVETLASWAGIPTSLLNTLPAEVQALSGVIDWS
                     APVEAAAVLDRHSKRKSAAPEGVFSVGITSLAKALDLAREHGAKPAEVSPSIYRLDFD
                     EEFSCALAPALGPSRLRLVCGNGWTAVEDLLPYATRGLPTQPLAQQDVYLALNAAPLQ
                     ARYGEEIGSIRLLTGLLLRQIQSDNPRLDRALTDSAYAIADELKALALDAQRFELTGH
                     LDTTKNNLSLGYALQFAGEQSFFVQINKDLAARARPAPEVWFSLPKTA"
     gene            complement(1222..3141)
                     /gene="dnaK"
                     /locus_tag="VFQ61_13240"
     CDS             complement(1222..3141)
                     /gene="dnaK"
                     /locus_tag="VFQ61_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET9955471.1"
                     /translation="MARIIGIDLGTTNSVVAVMEGKEPKVIVNEEGSRLTPSVVAWDD
                     RGEILVGQIAKRQAVTNPENTVYSSKRFIGRRFEEVEKETSRVPYKVVKGKNGDAWIE
                     ARGKAMAPPEVSAKVLMKLKKAAEDYLGEKVTEAVITVPAYFNDSQRQATKDAGKIAG
                     LEVRRIINEPTAAALAYGLDKKKNEIIAVYDFGGGTFDISILEVGDNVVQVISTNGDT
                     HLGGDDVDHLIMEWLIGEFRKDTGIDVSKDKMVIQRLKDASEQAKIELSSKQETTINL
                     PFLTADASGPKHLSKQLTRPKLEQMIRPLVDRSMEPVRRALDDAKKKPSEIDEVVLVG
                     GSTRIPLVQETVQKFFGKEPHRGVNPDEVVAVGAAVQGGVLAGDVQDVVLLDVTPLSL
                     GVETLGGVMTVMIPRNTTIPTQKKEIYSTASDNQTSVEIHVLQGERAEARYNRTLGKF
                     HLEGLPAAPRGVPKVEVTFDIDANGILSVGAKDLATGKDQRITITASSGITQDDIERM
                     VKDAASHEAEDKRRREEIDRRNKLDSMCYTLERQIADNKDKLGGVDVSSIEKLIKEGR
                     EAIEKQDDAKVSDVLTRLEKEAHNMAAKLYESAGAAPGGAPGAGAPGAGAGGAASGGE
                     GKKGDVIDAEFEETN"
     gene            3437..3509
                     /locus_tag="VFQ61_13245"
     tRNA            3437..3509
                     /locus_tag="VFQ61_13245"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3470..3472,aa:Ala,seq:cgc)
     gene            complement(3659..>4213)
                     /locus_tag="VFQ61_13250"
     CDS             complement(3659..>4213)
                     /locus_tag="VFQ61_13250"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9955472.1"
                     /translation="CEGDSGGPALDADGKVVGVVSRSGDACEEPVYGTVTAWRDLIIR
                     VAKRAATLGGYEPPSWVQTGSSEGPNVEPPCEGCAGNGPENGFGGRGTLPSGQGGSTG
                     TGSSARPSGGSVASGGRPSMTGVPATLPASGASDSEPTPDEELEASCNMGHAPPTATS
                     KMGVLLGVVGVGALRRRRRVEKRR"
BASE COUNT          702 a         1443 c         1225 g          843 t
ORIGIN      
        1 cgcggtcttg ggtagcgaga accagacttc gggcgccggc ctcgcgcgag ccgcaagatc
       61 cttgttgatc tgcacgaaaa acgactgttc gcccgcgaac tgcagcgcgt atcccagcga
      121 caggttgttc ttggtcgtgt ccaggtgccc cgtaagttcg aagcgttgag cgtccagcgc
      181 aagcgccttg agctcgtccg cgatcgcgta ggccgagtcc gtcaaggctc gatctagcct
      241 tggattgtcg ctctgaatct gccgaagcaa cagcccggtg agcagccgaa tggagccaat
      301 ctcctcaccg tagcgggctt gcagaggcgc cgcattgagc gccagataaa catcctgctg
      361 cgcgagcggc tgcgtcggca gcccgcgcgt tgcatagggc agcagatctt ctaccgccgt
      421 ccaaccgttg ccacacacga gccgcaagcg gctcggtcca agtgccggag ccagcgcgca
      481 actgaactcc tcatcgaagt ccaagcggta gatagagggc gagacctccg cgggcttcgc
      541 gccatgttct ctcgccaggt ccagggcttt cgccaaactc gtgatgccaa cggagaacac
      601 gccctccggc gcggcgctct tccgtttgga atgccggtcg agcaccgcgg ccgcctcgac
      661 cggcgcgctc caatcgatta cgccagacag cgcttggacc tcggcgggca acgtgttcag
      721 cagactcgtg ggaatgcccg cccacgacgc caaggtctcc acgaaactct gcgcgcgcgc
      781 cacccgcgcc acggccacta agtccggcgg ttgcgctacg ggcgagagat cgggctgggg
      841 cgctgcggtt gcagccggtg cgctctccac gttgagcggc gtggttgccg gcttgttcgc
      901 ccctccaccg caagcggaca caccccaagc gagaaccaga agcccagtgc ccaacgacgc
      961 gcgcagatgc atgtgccggg cactgtatcc gagttaggcg caattttcgt ccacgaaatg
     1021 gcccctacat gcgcctcccc cgagcccagc cggaagaact tgcctaccaa tgaaaacgcc
     1081 cgcgaactca atcgagatcg cgggcgcctc tgggctgctt cgttgcctgg cccgccggcg
     1141 cgtgcggcgg accaagctag tttcttcgct cgagagccgt tgctcggctc tcgatgctga
     1201 gcgggagctc agttccttgc ttcagttcgt ttcttcgaac tcggcgtcga tcacatcgcc
     1261 cttcttgcct tcgccgccgc tggccgcgcc acccgcgccc gcacccggag ctccggcgcc
     1321 cggcgcacca ccgggggcgg cgcccgcgga ttcgtagagc ttggccgcca tgttgtgcgc
     1381 ttccttctcc aagcgagtga gcacatcact gactttggcg tcgtcctgct tctcgatcgc
     1441 ctcgcgcccc tccttgatca acttctcgat cgaggagaca tctacgcccc cgagcttgtc
     1501 cttgttgtcc gcgatctgac gctcgagcgt gtaacacatg ctgtcgagct tgttgcgccg
     1561 atcgatctct tcgcgacggc gcttgtcctc cgcttcgtgc gaggccgcgt ccttgaccat
     1621 gcgctcgatg tcgtcttgcg tgatgccgct cgaggccgtg atcgtaatgc gctgatcctt
     1681 accagtggcg agatccttcg cgcccacgga gaggatgccg ttcgcgtcga tgtcgaacgt
     1741 aacctccacc ttcggcactc cgcgcggagc ggccggtagc ccttcgaggt ggaacttacc
     1801 gagcgtgcgg ttatagcgcg cttctgcgcg ctcaccctgc agcacgtgaa tctcgacgct
     1861 ggtttgattg tcgctggcgg tcgagtagat ctccttcttc tgggtcggga tcgtggtgtt
     1921 gcgcgggatc atcaccgtca tcacgccgcc cagcgtttcc acgccgagcg acagcggcgt
     1981 cacgtcgagc agcacgacgt cttggacatc acctgccaga acgccgcctt gcacggccgc
     2041 acccaccgcc accacctcgt cggggttcac gccacggtgg ggttccttac cgaagaactt
     2101 ctggaccgtc tcctgcacga gcggaatacg tgtggaacca ccgaccagaa cgacctcgtc
     2161 gatttccgac ggcttcttct tcgcgtcgtc cagagcgcgt cgcaccggct ccatcgagcg
     2221 gtcgaccagg ggtcgaatca tctgctcgag cttcggccgc gtcagctgct tcgagaggtg
     2281 cttcgggccg ctcgcgtcgg cggtcaagaa gggcaggttg atcgtggtct cttgcttgct
     2341 ggagagctcg atcttggctt gctccgaggc gtccttcaga cgctggatca ccatcttgtc
     2401 tttggagacg tcgatgccgg tgtccttgcg gaactccccg atcagccact ccatgatcag
     2461 atgatcgacg tcgtcaccac cgaggtgggt gtcgccgttc gtggaaatca cctggacgac
     2521 gttatcgccc acttcgagga tcgagatgtc gaacgtgccg ccaccgaagt cgtagacggc
     2581 gatgatctcg ttcttcttct tgtcgagtcc gtacgccaac gccgcggccg tcggctcgtt
     2641 gatgatgcgg cgcacctcga ggccggcgat cttgcccgcg tccttggtgg cttggcgctg
     2701 cgagtcattg aagtaagcgg ggaccgtaat cacggcctcg gtgaccttct cacccaagta
     2761 gtcttccgcc gccttcttga gcttcatgag caccttcgcg ctcacctcgg gcggggccat
     2821 cgccttgccg cgagcttcga tccaggcgtc cccgttcttg cccttcacga ccttgtaggg
     2881 cacccgcgag gtctctttct ctacctcttc gaagcggcga ccgatgaaac gcttcgacga
     2941 atagaccgtg ttctctggat tggtgacggc ctggcgcttt gcgatttgcc ccaccaggat
     3001 ctctccacgg tcgtcccagg caacgaccga aggcgtcaaa cgtgatccct cttcgttcac
     3061 gatgaccttg ggttctttgc cctccatcac cgcgacgacg ctgttggtgg tacccagatc
     3121 gataccaatg attctggcca ttttgttctc ctaaaagtgc gcggttcgcg cccgtcgtgt
     3181 tggatgacga aacgggacgc cggcccgtgg ttccgccgga ccgtcgaatt cgtgcggcaa
     3241 cataaccacg cggatcccga ggtcaactgc gttcctcaac cctgtttctc gacccctccg
     3301 agaactcgtc cactcaaccc cagcgcgatc gacacccccg gcgctcgaac ccgacgcgac
     3361 aaaaaatcac cacgcgcgct cagtaacccc attgacaggc gcggcaggaa gactaagttc
     3421 gcgggccttc actcccgggg ccgtagctca gctgggagag cgccgcgttc gcaatgcgga
     3481 ggtcaggggt tcgatccccc tcggctccac cggaatcacc cgatcccgga tcggaaaata
     3541 ctcatctgaa tcttttcgac gttcggatga gctgcacgga agaaaggctc gcgtttacca
     3601 cgcgggcctt tcttgttttc aactcatcgc ttcggcaacg agcgaaatgc agctgtgttc
     3661 agcggcgctt ctcgacgcga cgacgacgac gtagagcgcc cacgccgacc acgcccaaca
     3721 gcacgcccat cttgctcgtc gcggtgggcg gtgcgtgtcc catgttgcag ctcgcctcga
     3781 gctcctcgtc gggcgttggt tcggaatccg aagcccctga tgccggcaag gtcgccggaa
     3841 ctccggtcat ggaaggtctt ccgccagatg ccactgaacc gccactaggg cgggcagagg
     3901 aaccggttcc ggtcgagccc ccctgcccgc tcggcaatgt gccccgaccg ccgaaaccat
     3961 tctctggacc attgccggcg caaccctcac atggcggttc gacgttcggc ccttccgagc
     4021 tacccgtttg cacccagctc ggtggttcgt agcctcccaa cgtggccgct cgctttgcga
     4081 cgcgaattat caagtcgcgc cacgccgtga cagtcccgta gaccggctcc tcgcaggcat
     4141 ctccgctgcg ggagaccacg cctacgactt ttccatccgc gtccagcgcg gggccacccg
     4201 aatccccctc gca
//
LOCUS       DASTKU010000386         4550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_740991, whole genome shotgun sequence.
ACCESSION   DASTKU010000386 DASTKU010000000
VERSION     DASTKU010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740991"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..89
                     /gene="rrf"
                     /locus_tag="VFQ61_13255"
     rRNA            <1..89
                     /gene="rrf"
                     /locus_tag="VFQ61_13255"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(779..2080)
                     /locus_tag="VFQ61_13260"
     CDS             complement(779..2080)
                     /locus_tag="VFQ61_13260"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9955473.1"
                     /translation="MSSLAEAPLALFVLQAISIVGLSRLLGSVARAIGQPMVVAEIAV
                     GVALGPSLFGWLAPGAFAAVFPPSSLAPLGLLSQVGLLLFMFLIGLEFDPQLLKGRGR
                     ASIAISHASIAAPFGLGTLLALYLYPRVAPAHVSFLPFALFLGAAMSITAFPVLARIL
                     VERGLLDSRVGMLTLACAAVDDVTAWCILAFVVAVVRESGTSAFWTCALALAYIGFMC
                     VVIRPLLMRLARYGEAKGHGATDNYLAVILLLALLSAWITERIGIHALFGAFLLGAVA
                     PRTDGFAKRVAGKLETVVVVLLLPLFFAFSGLRTDLQLLNSPESWWMCGLIVFVACLG
                     KFGGSSVAARLSGLSWREASTLGILMNTRGLMELIVLNTGLELGVISPKLFTMMVIMA
                     IITTFMTTPLVLKLYPRRLAKPAEDVASFTLLESPVREPSP"
     gene            complement(2250..3506)
                     /gene="fabF"
                     /locus_tag="VFQ61_13265"
     CDS             complement(2250..3506)
                     /gene="fabF"
                     /locus_tag="VFQ61_13265"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET9955474.1"
                     /translation="MLEPVSGGTRRRVAITGLGTVNPCGNDMASTWRSVLEGRSGIGP
                     ISRFDASTLESRIGGAIQAFDASRYLTEKEQSRCDLFIQYAVAAAAEAMASSGLIETR
                     LEPEDREMFGVMIGSGLGGLGTVESSALAPRLSGMFYPATLVNLASGQVSIKWDLRGP
                     SGAVSTACATGAHAIGDAARLIMHGYATRMLAGATEAPLSRLGVGGFDALRALSRRNT
                     EPARASRPFDRDRDGFVISEGAAVLVLEEYESARARGAPILAEIVGYGSSSDAHHVTA
                     PRPDGAGAASAMRRALADARVAPSQVGYLNAHGTSTQLGDRVECQAIRSVFGADTQRL
                     KVSSTKSTTGHLLGAAGALELALCALALRARMAPPTINVENLDPACELDVTPNVPSRI
                     DSEYALSNNFAFGGLNASILLKAHSR"
     gene            complement(3499..3768)
                     /locus_tag="VFQ61_13270"
     CDS             complement(3499..3768)
                     /locus_tag="VFQ61_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET9955475.1"
                     /translation="MLDLRVRELIAAQLRRQHEEIRDELHVADDLGADSLDQTELLLA
                     FEQEFDLKLEMPATGLGTVEQTIERLRRALEGQAVLDQEQSSSRA"
     gene            complement(3875..>4550)
                     /locus_tag="VFQ61_13275"
     CDS             complement(3875..>4550)
                     /locus_tag="VFQ61_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HET9955476.1"
                     /translation="GLSVWMPSFFGTAGRKLSGPYIAGSMLRACVMREFSVFKKNTNS
                     PVTSWLRALARHVHRECGGRGVGALGMCLTGGFALAMMVEESVIAPVLCNPSLPFALT
                     PARKGDLGVDSATLARARARSIEGGVCLMGLRFTEDIAVPHERFQRLREEFGEHFIAI
                     EINSGSGCAEGLSRLSHSVLGADFVADPQHPTALAESRAVTFIRDRLMAPPQRTSPGL
                     ELATAD"
BASE COUNT          903 a         1418 c         1419 g          810 t
ORIGIN      
        1 ccgatcccat ctcgaactcg gaagtcaagc tcttcggagc cgatggtact gcacgggcaa
       61 ccgtgtggga gagtaggacg ctgccgggct aattgcctga aggggccctg aggtaacact
      121 cagggccttt tctttatcta atgagtaggt cggctggacg gttgaattcg gttgcaacgc
      181 acgctcattg agttcctagc tgggaagttt atcgccgctt tctggggcgg accccggatc
      241 gtctttggcc ttctcttggg gtttttcatt gggtcgggct cgttgttctt gcccgctctg
      301 acgttggtcc ccttgctgtt cagtcgactg tttctggctg tccccggcag tttgattttg
      361 gttcatatcg tttctcctca ccgggaccac tgcagagccc gtgccatgcg ttgcgcgagc
      421 cccttgcgag gcacgccatg tttttgggaa acatcaccgc aggtcgcggt ggtccgtctg
      481 ggagacagcg agctcgcgtg gtcgagatcc gcgtgattgc cggcaagcgc gtgagcgctg
      541 cgcgtgagct ccccggtcga ggcggacgag tcggcagttt tggggcgcgt agcgtgagcg
      601 gcgagcgcgg ccggctgcga cgaataggcg cgtgtaagcc aaacttgcag cccccgctcg
      661 gttctcaagc agggctcgtg gtaactctag tcactcgcgc aacggtgctt tcttgccgat
      721 gtccggggtc ccttggacga tcgattccca gtaaacctcg aggatcacgg agagcgcttc
      781 acggagacgg ctcacgaaca ggggactcga gcagcgtgaa gctcgcgacg tcctctgccg
      841 gtttggccaa cctgcgcggg tagagtttca ggacgagggg tgtggtcatg aacgtcgtga
      901 tgatcgccat gattaccatc atcgtgaata gcttcggaga gatgaccccg agctcgagcc
      961 ccgtgttcag aacgatgagt tccatcaggc cacgggtatt catgaggatt ccaagcgtgc
     1021 tcgcctctcg ccaactcagc cccgagagcc gagctgccac gctgcttccg ccaaacttgc
     1081 cgaggcaggc gacgaagacg atgaggccgc acatccacca ggactcgggg gagttcagca
     1141 gctgaagatc ggtccgcaga ccgctgaacg cgaagaacag ggggagtagc aacacgacca
     1201 cgacggtctc gagcttaccc gccacccgct ttgcgaaccc atcggttctc ggcgcaacgg
     1261 cgcccaacaa gaacgcgcca aacagcgcgt gaataccgat gcgctccgtg atccacgcgg
     1321 agagcagcgc gagcagcaga atgaccgcga ggtagttgtc cgtcgcgcca tggcccttgg
     1381 cttcaccgta tcgcgccagc cgcatcaaca gcggtcgaat gacgacgcac atgaaaccga
     1441 tgtaggcgag ggccagggcg cacgtccaaa aggcggaggt gccgctctct cggaccacgg
     1501 cgaccacgaa ggccaggatg caccaagccg tgacgtcgtc gacggccgcg caagccaacg
     1561 tgagcatgcc cactcgcgag tcgagcagcc cacgctccac caggatccga gccagtacgg
     1621 ggaacgcggt gatgctcatc gccgcgccga gaaacagcgc gaacggaaga aacgatacat
     1681 gtgcgggcgc aacacgcggg taaagataga gggccagcaa cgttcccaag ccgaacggtg
     1741 cggcgatgct cgcgtggcta atcgcaatcg atgcccggcc tcgtcccttg agcagttgcg
     1801 ggtcgaactc caggccgatc aagaacatga acagcagcag acccacctgg ctgaggaggc
     1861 ccagaggagc caatgagcta ggtgggaaga cggctgcaaa cgcacccgga gcaagccagc
     1921 cgaagagtga cgggccgagt gcgacgccta ccgcaatttc cgccaccacc atcggttggc
     1981 caatggcgcg agccaccgac cccaaaaggc gcgagagccc gacaatcgag atggcttgca
     2041 ggacgaagag cgcgagaggc gcttcagcta aagagctcat gcgccgtccg caaccgcgtt
     2101 gggatacgat cgacgggggg atcggcgaga gtggacggcg aaaaactggt gctgcatgtc
     2161 acctcggcgt ggcgtggcgt ggacatggcc agtcgctggg aaagaagcgg caacgaactg
     2221 gcgcatggca agggctcttt tcgggtccac tatcgagagt gggccttcag gaggatgctt
     2281 gcattcaagc ccccgaaagc gaagttgttg ctcagcgcat attcggagtc gatccgactc
     2341 gggacgttgg gcgtgacgtc gagctcgcaa gcggggtcga gattctcgac attgattgtg
     2401 gggggcgcca ttcgagctcg cagtgcgaga gcgcaaagcg cgagctccag agcgcccgcg
     2461 gcccccaaga ggtggccggt tgtagacttc gtggagctca ccttcagacg ctgagtatcg
     2521 gcgccgaaca cgcttcgtat ggcctggcat tccacccgat cgccgagttg cgtggacgtt
     2581 ccgtgagcat tcaaatagcc gacctggctg ggcgcaactc gcgcgtcagc gagcgccctt
     2641 cgcatcgcgg aggcggcccc cgccccatcc ggccggggtg ccgtcacgtg gtgagcgtcg
     2701 ctgctggacc cataaccaac gatctcagcc aaaatgggcg cacctcgagc gcgagccgac
     2761 tcgtattctt ccagtactag tacggcggcc ccttcgctga tcacgaagcc atcccgatcc
     2821 cgatcgaacg gacgtgacgc tcgggcaggt tcggtgtttc gccgggagag agcccggagc
     2881 gcatcgaacc cacccacccc gagtcgtgac agaggcgcct cggtggctcc cgccagcatc
     2941 cgcgtggcgt agccatgcat gattaaccgg gccgcatcgc cgatggcgtg ggcaccggtc
     3001 gcgcaggccg tcgaaacggc acccgaaggc ccccgcaagt cccacttgat cgaaacctgc
     3061 ccagacgcga ggtttacgag cgttgccggg taaaacatcc cggacaatcg cggggcgagc
     3121 gcgctcgatt ccacagtccc gagcccgccc agccccgatc cgatcatgac cccgaacatc
     3181 tcgcgatctt cgggctcgag gcgggtctcg atcaatcctg aagatgccat cgcttcggcc
     3241 gccgccgcga ccgcatactg aatgaacaaa tcgcagcggc tctgttcctt ttcggttaga
     3301 tatcgcgatg catcgaaggc ctgaatcgcg ccgccgatgc ggctctctag ggttgacgca
     3361 tcgaagcgcg atatcggccc tatgccactt cgcccttcga gcaccgaccg ccaggtgctg
     3421 gccatatcgt tgccgcacgg attgacggtt ccgaggccgg taatggcaac acgacggcgc
     3481 gtgccgccac tgactggctc aagcacggct gctactctgc tcctggtcga ggacagcttg
     3541 cccctcgagc gcgcgtcgca gtctttcgat ggtttgttcc accgttccca acccggtggc
     3601 tggcatctcc agcttcaaat cgaactcctg ttcgaacgca agcagcagct ccgtttgatc
     3661 cagagagtcc gcgccgagat cgtcggccac gtgcagttcg tcgcggatct cctcgtgttg
     3721 ccggcgcagc tgcgcggcaa tcaactctcg tactcgtaag tccaacatat tcggctcctc
     3781 tttggggtat gcaattgggt tgggttgcta accggtgctg gtgccctgga ttcgacgggc
     3841 acccgcaagg gattggaact gcactgagca ggactcaatc ggcggtggcg agttcgagac
     3901 cagggctagt tcgttgaggc ggcgccatca agcggtcgcg aatgaacgtg accgcacgtg
     3961 actcggctag tgccgtcgga tgctgcggat ccgcgacgaa gtcggcgccc aaaaccgagt
     4021 gagacaatcg gctcaggccc tcggcgcagc cggatccaga gttgatttcg attgcaatga
     4081 agtgctcacc aaactcctct cgtagcctct ggaagcgctc gtgcggaaca gcaatgtcct
     4141 cggtaaaccg taggcccatc aagcagacac ccccttcgat actcctcgcg cgggcgcgag
     4201 ccagtgttgc ggagtctacc ccaagatcgc cctttctagc gggagtgagg gcaaacggaa
     4261 gggacggatt gcacagcacc ggagcgatga cgctctcttc gaccatcatc gccagtgcaa
     4321 agccacccgt gagacacatg cccaaagccc cgacacctcg tccgccacac tctcgatgta
     4381 cgtggcgcgc cagtgcgcgc agccaactcg tgactgggct attcgtgttc ttcttgaaga
     4441 cgctgaactc gcgcatgacg cacgcgcgga gcatgctgcc cgcgatgtaa ggcccgctga
     4501 gcttccgacc ggcggtgcca aaaaacgagg gcatccacac gctcagccct
//
LOCUS       DASTKU010000387        25562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1858075, whole genome shotgun sequence.
ACCESSION   DASTKU010000387 DASTKU010000000
VERSION     DASTKU010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 25562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25562
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858075"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="VFQ61_13280"
     CDS             complement(<1..1585)
                     /locus_tag="VFQ61_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955477.1"
                     /translation="MPRDAHIPLFLWVATALLIHLLGGNGAHRAADWMEERIELKRFV
                     EGVRSYVRSSTDTLEVSLVEDQVTPPEPERDPQTPQDAEADPKDETTEPEEKPKTAPP
                     KAPDPKKPEPEKDTPKPEERKPEPEPEPKPVIVEKKQPVEAQPLVERKQVAVRQHVED
                     KDQKDNPNADFISEHNNRVKEQTQSRLTSNDENDPKPTPGSNASGVGPEPGDSRENRI
                     AQSEDRPGEEARAPADDPASRKAQPSSESPNAGDVTARAQAHAAPARNGSQAGENKTE
                     PQQAVAQNSTPGSKGQAEQRAQEASPDWVGSERGGWDVPNARAELKEQEARKSQRKRE
                     LPRRKVRGYGDLLGLGAAGTTANGINLNLTPGLAVNAIGVDQLSRERRADAARRRSQH
                     LGSWKSTGIERWRSAIENYVASVKPGNQTALNTARSPFASYLASIHNRLHPIFADWFL
                     ASLDSLPQSHPMNNPTISTSLEIILDQDEGRIVNMGVTKTSGITAFDIAALDSVQRAS
                     PFGAPPREILSPDGNVYLHW"
     gene            complement(1677..2528)
                     /locus_tag="VFQ61_13285"
     CDS             complement(1677..2528)
                     /locus_tag="VFQ61_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9955478.1"
                     /translation="MTLLEGKVAIITGAGAGIGRATALLFAKEGASVVVNDVGAARDG
                     RGNDPGAAELVVDQIRAQGGTALASQHDISSPEGANELVRLAQEQLGGLDVLVNNAGI
                     LRDKTLLNMELADFEAVLRVHLTGSFLCLKAAAQVMKEQGRGGSIVNTTSVSGLLGNF
                     GQANYAAAKAGIYGLTRTASIELQRYSIRVNAVAPVAKTRMTEDLPMFAKIENTLTPE
                     HVAPAHLFLASDLARDVSGAVLSVAGARLSVYKVVESSGKFKESEDGVWTAREISDHF
                     AGISKLP"
     gene            complement(2545..3060)
                     /locus_tag="VFQ61_13290"
     CDS             complement(2545..3060)
                     /locus_tag="VFQ61_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955479.1"
                     /translation="MNYAGRSQRPRARRRRTLSSFPAFGRGLSIGLCLAGLAWVSPAR
                     AEDRFFGPDKTLHFGASMGLAAGGYAVSGLIWEEPWQRSVAGASLALSAGAAKELWDL
                     SGHGDPSWNDVAWDVIGTAVGVGLVLCVDLVLRHAEDRDGNRKQPSRTGATLGTPPLL
                     QKSQAKFQLRW"
     gene            3115..5187
                     /gene="metG"
                     /locus_tag="VFQ61_13295"
     CDS             3115..5187
                     /gene="metG"
                     /locus_tag="VFQ61_13295"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET9955480.1"
                     /translation="MTRRILVTSALPYANGHIHIGHLVEYLQTDMWVRFQRLRGHRVI
                     YMCADDTHGTAIMMRAQKEGKREEDVIAEMSSAHQRDFAEFEVAFDHYGSTNSEATRK
                     LCHEIWASLRRASLVKEREVTQLFDTKAGVFLADRFVKGRCPRCGAPDQYGDSCDKCG
                     STYSATELLEPVSALSQTTPEIRKASHLFVEIEALHGFLEEFVATPERLQAEVANYLK
                     GHFLSEPLRDWDVSRPAPYFGFEIPDAPGHYWYVWFDAPVGYLGATAEWCEKNGESFD
                     AWWRSADTEVHHFIGKDITYFHTLFWPAMLQTAGLTLPTRVQVHGFLTVNGEKMSKSK
                     GTFVLARTYLDALDPAYLRYYYASKLGPRVEDLDIALDEFVNKVNSDLVGKVVNLASR
                     TARFVEGTGLSKSYPDDGGLFAEGAAKGEEIAAAYEVRDFAKAMRIVMGLADRANEYV
                     DRMEPWKLKKDPARGAELQAVCSVVLNLYRQIIVYLAPVLPKLARDSAALLNAPITRF
                     EDAAVPLCGTPVAAFSHLMQRVDPKKLESVIELSRETPVMSNPSSVQNTEAQPVTSTA
                     TSTDDGSALAAEPLAAECSIEDFQKVDLRIARIVAAEHVPEAKKLLKLTVSLGGEDRR
                     TIFAGIKAAYAPEALVGRLVVVVANLAPRKMKFGVSEGMVAAAGSGGDQVFLLSPDAG
                     ARPGHRLH"
     gene            complement(5301..5777)
                     /locus_tag="VFQ61_13300"
     CDS             complement(5301..5777)
                     /locus_tag="VFQ61_13300"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9955481.1"
                     /translation="MSLDRNREVLAHLYGSFQRRDAQGMIDCYAEDAVFEDPVFGQLD
                     HELLTRMWRMFCERGQDLEVSFVVSPTENGGTVEWDARYTFRKTGRKVHNRIASLFEV
                     RDGKIVKQKDSFSLWRWSRMALGATGILFGWAPPVQRAIRSAAQKDLRSYSSSVSN"
     gene            complement(5817..7079)
                     /locus_tag="VFQ61_13305"
     CDS             complement(5817..7079)
                     /locus_tag="VFQ61_13305"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9955482.1"
                     /translation="MTDLENWFADVGAKLTGWLGSPEVLRVVTKGLRRCAPTIVLHGR
                     AFVTLRQDVLEVLSNDRDFGVTEVYDAKMRRTSGAFVLGMENTPQYRREAAFIRAAVH
                     AEDMSAIRASVGAHADELVTRAQPQGKIDAVEGFSHLIPLRLLRDYFGTPGPDWATLA
                     RWMRSIFWEIFLNFTDDVEVAQEAERSSAELRSYLENLVAQRKRELSEGQLKTDDFVS
                     RLVKQQQLAGARFEDGTPIDDATLVRNIGGVIVGAVDTQSKAITHALEQLIEHPGALR
                     AAQQAALDGDRELVAAYVWEALRFNPHNPILIRFCRNGAVIGAGTEREVVIPSGTTVF
                     VSTLSAMFDESVLDEPEVFRPGRPREHYLHFGHGQHRCFGEHINRVVLPEVIQRVLVL
                     RKLRVQPGEHVEYVGPFPKRFALEFDPS"
     gene            complement(7109..8020)
                     /locus_tag="VFQ61_13310"
     CDS             complement(7109..8020)
                     /locus_tag="VFQ61_13310"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9955483.1"
                     /translation="MKPRIKNLVFGGTGVRNAAYVGGILALEEAQLYREVEAFAGTSS
                     GSIIATLASLDYSPAEIRQHVLDLDFNRLQDGSVLGGPFRVLKRYGWHKAEYLTQTLG
                     ELIARKVNNPRASFADLRDAGAKRLKVIGTNLTRRSVRVFPDDDAWHMPVVDAVRISI
                     AIPLFFEACEFENEVYVDGGVLWNLPLEAFDTAEGANFETLGFLMSTKAATPRPLRSM
                     HDYVECLFGAMLNEQEVDLAHSPANRSRVIRIDDLGIPTTNFGITQEEKLALMERGAA
                     ATREYLSARPNYAPASGEPTIERRPVT"
     gene            8409..9707
                     /locus_tag="VFQ61_13315"
     CDS             8409..9707
                     /locus_tag="VFQ61_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955484.1"
                     /translation="MRQQTLTVVMPVRGTLELREALRARAALHEQLRQLLAQPALGIH
                     FARLAFLDEPEAGKHPSCFVFESNFDTKIADPREAMQEHLRALCELGTPAIADWLHYS
                     ESKLESASGRALADYLERHLVEATACYQGHVQRDLGRIRAEQALRDVIFTYLEGCSGH
                     APHVLYRKIREHVRERATRDPRLSGLDFDAPAPAQPDLEARSRALKSPVWPWIRNSDV
                     LLPAVLRLGRVAVWGKTDSYYELRKRQEAWTDRDREVFRDISRTEDFGAQNAITHVVS
                     LRDGDGRLGVMRTAHRYIARLAERYFNDVGQLGGIPSIHFAKWLLLDGGKRLLFLSNY
                     DSSWESYLGDFVDQAWIGLNLAWSCTAEYPATRHLVLGGAMDEERFKAWGRSCQRPTE
                     LFYSAYPDLSVAAINNNTAIRAGLHAPAPPDLDAWFRRLT"
     gene            9730..11244
                     /locus_tag="VFQ61_13320"
     CDS             9730..11244
                     /locus_tag="VFQ61_13320"
                     /inference="COORDINATES: protein motif:HMM:NF044706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955485.1"
                     /translation="MRTSLDLPDIQGLILSGYGDRPAARYGLFEIVDVARARRWLASL
                     VPELQFGEYRRSARHEQPLLKPLCANVAFTAAGFRTLGLTETALAGFSLAFQEGMNEP
                     ERARRLGDDGESAPERWEWGYPGQALHGVLAVFAGRDDAEEAEWEVIRAFLERQLCAE
                     HGVKALRVLDTLATNRASRREHFGFRDGITNPRIAGLSRGRENLIEPGEVLLGYKNGY
                     GHYPLSPEVQSSEDPDGRLPVSLELETRRDFGRNGSYLVFRQLRQNVSAFWAWMQETS
                     ARIPDAPRGVQGAVWLASKVIGRWPNGTPLTIYPEAPGPDAAAENDEFAFGEHGDAYG
                     SRCPIGSHIRRTNPRDTALPVPHDPELSGTPEDSGTKRRRMKLSDLHRILRRGRLYGD
                     PCHDRFDPSELCSGKDDGKDRGIHFLCFNANLSRQFEFIQSNWVNNPCFAGLSSDPDP
                     LLGARRAYPFGASDFTIQGYPPRRVRNLPRLVEVKGGAYFFMPGRSALLYLSRA"
     gene            complement(11294..12589)
                     /locus_tag="VFQ61_13325"
     CDS             complement(11294..12589)
                     /locus_tag="VFQ61_13325"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET9955486.1"
                     /translation="MELIRRKRDGGELSPEELRWMISGLLAGSVTDYQISAFLMAVYL
                     RGMSDAETVALTHVLRDSGKKLQLGSVKRPKIDKHSTGGVGDKISICLAPLVAACGVA
                     VPMISGRGLGHTGGTLDKLEAIPGFDVHLPARRFEQLVRNVGTCLIGQTDELAPADRK
                     LYALRDVTATVESIPLIVASILSKKLAEGIEGLVLDVKVGRGAFMKDEASARRLAKAL
                     IRVGRLSGIRVVALLTEMSTPIGLTVGNALETREAIDVLKGGGPSDTRELTLALGAEM
                     LRLGGVSTSAAQARSTLLGAIASGRALECFARVVHEQGGDASVIEHPERLPQARKQML
                     LKASEAGVVTEIDALELGLAAVELGAGRTRADQAVDPGAGIEILAPLGTRVRAGDALA
                     RIGAARTTLLDSAAARVSASFKLGKRARPACDRVLGVLR"
     gene            12831..13856
                     /locus_tag="VFQ61_13330"
     CDS             12831..13856
                     /locus_tag="VFQ61_13330"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing transcriptional regulator"
                     /protein_id="HET9955487.1"
                     /translation="MTETSRGRPSGKFTQHRRLDRLREILERHPRGLSLYELADALSV
                     TVRTMRRYLIEMEREYDLESIPTRGGGPLLWRIRPGEMPRKVELRRTQAYALLAARRL
                     FEPMRGSALFEEIDLAIGKLLSFAQRPGRGPNAGLADARLENRFLYLPHAPKNYTEKT
                     EELDDLFQAVSDLRPLTMTYRSAGKAEEERITIHPYALVLHRDSIYCVAFHVGRGEVR
                     TFVLDRMRDTECATTERFDLPEAFAVDEYFQGEFGIWKGSTPQRVVIEFDATAAEYVR
                     MRKVHATQKLSQIQGGGVRLTMTVGNLNPVVSWVLEWGHRARVISPPELLDRVREELG
                     RALGNYS"
     gene            complement(13979..14617)
                     /locus_tag="VFQ61_13335"
     CDS             complement(13979..14617)
                     /locus_tag="VFQ61_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET9955488.1"
                     /translation="MSFPRPHRSGSHRATGWALTLGLFLALTSDAAFADIYRSVGPDG
                     VISFSNSSKSGKLYVRTPKDRTRVAMPSDSSSERFSRYDSHIREAAALYQIPEELVRA
                     VIKVESDFDPRAVSPANARGLMQLIPETAERMLVTDIFDPRENILGGTRYLRVLANLF
                     SGDIQLTLAGYNAGEGAVMRHGGIPPYPETRDYVSRVLAYYRQYSAQRAPAR"
     gene            complement(14874..16865)
                     /gene="speA"
                     /locus_tag="VFQ61_13340"
     CDS             complement(14874..16865)
                     /gene="speA"
                     /locus_tag="VFQ61_13340"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HET9955489.1"
                     /translation="MNGAKLSAISLDAALDVSRRSATVEKQNGSPEWTLDRAAQRYQI
                     HGWGAPYFQVNARGHVQVTPDPERQQHIDLYELTQELTARGLEAPLLVRFPDIVGDRI
                     KRINEAFARAIAEYDYQGTYRGVFPVKVNQQRHLVEEIVEVGRKWNFGLEAGSKPELL
                     IALACMSDVGGLIICNGYKDRQYIETALLAQKFDKTVIVVLERLEELELVFRASEKLG
                     IRPLLGVRAKLTTRGVGRWADSAGDRAKFGLNTSEMIEVVDRLTERNMLDSLQLLHFH
                     IGSQISSIIPIKNAMQEASNVYVELAKMGCRMGYLDVGGGLAVDYDGSKTDFHASKNY
                     QLEEYAADVVAHIQAACSKADLPVPTLVSESGRAIAAHHSVLVFEVVGRNEVRFGEPL
                     EPKPEAHRLLKELYETYHGIQPKNVQESYHDASQAKEEAQSLFKYGYLTLRERAQVER
                     LYWNCCEKLQQTLRRLNFVPEELKHLDRTLSAIYYCNFSLFQSAPDIWAIDHLFPIMP
                     IHRLDEEPTVSATLADLTCDSDGMIDRFIDVEDVRSSLDVHTLKDNSEPYYLGMFLNG
                     AYQEILGDLHNLFGDTHAVHVALTEDGGYHVRHVIKGDSVSEVLRYVEYQPDSMVDAV
                     RRQAERAVSAGRLTNDQLRTLMRHYEDSLRSYTYLTGDD"
     gene            complement(16996..18006)
                     /locus_tag="VFQ61_13345"
     CDS             complement(16996..18006)
                     /locus_tag="VFQ61_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET9955490.1"
                     /translation="MHALFSAWSGGLGFGPAKPLTPEVTTSLLPVERIEPASYFSALL
                     GYPPLKALLPIPILAALTPIVFWFFRRTWEEVNREATAERALAGPQMDYRPAACFVIT
                     AIVLTVQEYYGGRSFYDQVIRPALSAVNWGPEGAHRFAKYDELWGYLWWSSARVIGYV
                     LFPLPLWKLLFPKDSLLDMGLRLRGLREHVWIYGLCLAVVVPAMFIVAREPDFGSYYP
                     FYKLSSRSYADWFAWEAMYIVQFFALEVFFRGFMLSALRRTLGASAIFAMALPYCMIH
                     YGKPYLEANGAIVAGVVLGSLSMRTRSIYAGFLVHVTVALSMDILALSHRNALPHVWF
                     AP"
     gene            complement(18015..19448)
                     /gene="gltX"
                     /locus_tag="VFQ61_13350"
     CDS             complement(18015..19448)
                     /gene="gltX"
                     /locus_tag="VFQ61_13350"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET9955491.1"
                     /translation="MKPRLRFAPSPSGYLHIGGARTALFNWLWARKTGGTFVLRVEDT
                     DQERSSLDSVRAILDALKWLGLDWDEGPEVGGPYTSYFQSERKAAYREAVEGLIREGK
                     AYRCYCTKEELDRAREELKQKNPKAAFVYPGTCRERTDRPDLPHVVRFKTPRDGSTDF
                     VDKVFGAISTPNAAQQDFVLVRTDGYPLYNLAAVVDDHAMDITLVARGRDHIGNTPQQ
                     VLLYRAFGWEPPEFAHLPMMLNSKGEKLSKRHASVAVQDYRERGFTPMGVLNYLVRFG
                     WSHGDQEIFSRSELVQLFDWDHVNKSDGRFDERKFADVAFEHLKRDELTPLAEYEQLV
                     QPFLAAQGHQAVDPARLRAAIPGIRERARTLADAATDMDFYFRAEPNLDEKAKQKFLT
                     PESAPRLRGLEQALRSVADWSPAALEACGAAYAEAEKISLKEFAQAARVALTGRSASP
                     PLYDVMAVLGREATLARLTRGAALAAG"
     gene            19699..20232
                     /locus_tag="VFQ61_13355"
     CDS             19699..20232
                     /locus_tag="VFQ61_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955492.1"
                     /translation="MKKSPLSAVKERFNSKDELVAAVQKLATEDMWLDRVSSTKGLGR
                     VANSKLLHLHDVLSDAKSRFGSRAKLIDAILTLQKRVKDAGYKSRLEGYALPRLLDLH
                     GTLSRTEKRAQAKAAGKKAPKKAAAPVAPPAAPAKKAAAKSVAPKAAPAAKAAAAKPA
                     AAAKPAAKPAKAAKKKG"
     gene            complement(20414..20932)
                     /locus_tag="VFQ61_13360"
     CDS             complement(20414..20932)
                     /locus_tag="VFQ61_13360"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HET9955493.1"
                     /translation="MPDFRVEHTFNCSEDTFWSKVFLDEEYNRKLFLERLKFSAWREA
                     SREERDQKLHRVVEAAPPMGEVPAALKAVIGDNAGYQERGVLDRAAHRYTVEVVPNRL
                     ADKIQVKLEMITVADPSSPERCKRTVKGSVTAKIFGVGGLLEKKIIADLEKSYSKSAE
                     FTNQFIAEKGLR"
     gene            21006..21728
                     /locus_tag="VFQ61_13365"
     CDS             21006..21728
                     /locus_tag="VFQ61_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HET9955494.1"
                     /translation="MTVARDTAVLAPLRRRTWLAQARVILQKDVAIEVATGEVVITSG
                     FFALLVAIVASLSFYGGPAMQRTVGAGVIWVSLTFSAVLALGKTWQRERDESALEGLL
                     VAPLSRSAIFAGKALGVLVFLWVIAGLLIPLTALLFSLDLLEVGPGLFLIAAAATPGL
                     AASGTLFGAMTVRTQARDLLLAVVLFPLLTPTLLCAVAATRELFAGTPVGELADYFKL
                     LLVFDVVFVSGGLSLFGTLIER"
     gene            complement(21759..23075)
                     /locus_tag="VFQ61_13370"
     CDS             complement(21759..23075)
                     /locus_tag="VFQ61_13370"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9955495.1"
                     /translation="MSFRQSALEDSVVITHDGHALHARPGESLAHALIAADRLVLSRS
                     PKLHRPRGPFCLTGECDGCLLRVDGVPNVAACQHRVCGGEVLQTQNVLGSREVDLLQA
                     NDFLFPHGIDHHRLFAGVRGVSALVTRLARRIAGLGNLPDLPAELGLESSDESWPALI
                     IGGGVAGLSAARGLGEKALLVEMAPELGGVERLLNPAESVRRVEDARNSGAQLRTRAT
                     AIGIYEPAPERALDEGHADAPRAGESPRTFEVLVARPGGMLRVRTRCLILACGSHAVI
                     PEFANSDLPGIFSARAGLELLRSGISPGERVAVLGLGPVADCAARELGSQLAVRLQNL
                     ETVLGAEGRTRVSGLRFREHDEGRRLKVDAVLCDGPPAPSFELAVQAGARVQFNPDLG
                     FVPEAREHDHCVASGVYCIGAAAGAAEAAEHQGYEAGRTLGNALRG"
     gene            23215..23784
                     /gene="nadD"
                     /locus_tag="VFQ61_13375"
     CDS             23215..23784
                     /gene="nadD"
                     /locus_tag="VFQ61_13375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HET9955496.1"
                     /translation="MRVAVFGGSFDPPHVAHVLLATYALSVGGFDRVLVVPVFAHAFS
                     KSLSPFAQRLRMCQLAFAPLRAVQVSDIEATLPTPSFTLSTLQAIRRELPDASLNLVI
                     GADALSETSKWHAFDEVARIAPPFAVGREGYDASGASHFALPRVSSTQVRKLLTSSDR
                     SAQRAELSELLPRSVLEYIEQHGLYPPPP"
     gene            23896..25332
                     /locus_tag="VFQ61_13380"
     CDS             23896..25332
                     /locus_tag="VFQ61_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955497.1"
                     /translation="MDLRTRTSLFCGVLALAIAASILLRGRPRRAQILFAALAGDMGL
                     WYLAQWQYHSGRADFWERSTAVLAVLLPQFALHLFDAIVPRVGRRSALLRVAGLLCVP
                     MLGLVLTEHRHGLVRSAVIVYVFGLIAAGLWALAQRGEHSGSRATQRRVRLLVLIGAL
                     AAAFSFADFLWFIGAPLPPVSAVLSIVFLFALSESLIRARLVDLYDLLGQILVSTALA
                     FLLAGIFYVFVVLLGGFDTMYLGAILAAIVILVLFEPLREKAESYTHKVFFRERVDLE
                     RAVTRARRALIHVLDVNEMQQVVLSALEASRRATGAALYTRHPSGRDFELGASFGPVA
                     PPRIEVATAHPLFERLDSSASIAFEEVAQRVVERRRVGLIREAEADERLLAAAGVLGS
                     FAQGLCLGVYGERHELLAILLVVDDRVRDAFSSDEVGLLEALAVQMGVVIENSRQHRR
                     LQEQDRLAALGQMAAGLAHEVKNPLGAI"
     gene            25375..>25562
                     /locus_tag="VFQ61_13385"
     CDS             25375..>25562
                     /locus_tag="VFQ61_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955498.1"
                     /translation="MNEREFVGIILEEVERLDRVVGSVLNYARPSKGDVGAVDVNAVV
                     RRTLTVLASNPVQGCEIV"
BASE COUNT         4398 a         8133 c         8098 g         4933 t
ORIGIN      
        1 cccaatgcag atacacgttc ccatccggag ataggatttc gcgcggaggc gcaccgaagg
       61 gtgacgcccg ctgcaccgaa tccaaggcgg cgatatcgaa ggcggtgatc ccggatgtct
      121 tcgtcacacc catgttcacg atgcggccct cgtcctggtc cagaataatc tcgaggctcg
      181 tgctgatcgt ggggttgttc atcgggtggc tctgcggcag cgaatccaag ctggccaaga
      241 accagtccgc gaagatcggg tgaagacgat tgtggatgct cgccaggtaa ctcgcgaacg
      301 gcgatcgggc cgtattcaac gcggtctgat tgcccggctt cacactcgcg acgtagttct
      361 cgatcgcaga ccgccagcgc tcgatgccgg tcgacttcca cgagccgagg tgttggctgc
      421 gccgtcttgc cgcgtcggca cgccgctcgc gcgacaattg atcgactccg atcgcgttga
      481 cggcgagccc cggcgtcaga ttgaggttga tgccgttggc ggtggtgccc gcggcaccca
      541 gcccgagcaa atctccgtaa ccgcggactt tgcggcgggg caactcgcgt ttgcgctggc
      601 tcttgcgcgc ctcttgttcc ttcagctccg cacgcgcgtt cggtacatcc cagccgccgc
      661 gctcggagcc gacccaatca ggcgacgcct cttgcgctcg ctgctccgcc tgacctttgg
      721 agcctggcgt cgagttctgc gccactgctt gctgcggttc agtcttgttt tctccggcct
      781 gcgagccatt gcgggcgggc gcggcgtgcg cttgcgcgcg tgccgttacg tctcctgcgt
      841 tggggctctc gctgcttggc tgtgctttgc gcgaggcggg atcatccgcg ggagctcggg
      901 cctcttcgcc ggggcgatct tcgctctgcg cgatgcggtt ttcgcgcgaa tctcccggct
      961 ctgggccgac cccgctcgcg ttcgagccgg gcgtcggctt gggatcgttc tcgtcgttcg
     1021 aggtgaggcg ggattgggtt tgctccttca cccgattgtt gtgctcggaa atgaagtcgg
     1081 cgttggggtt gtccttttgg tccttgtctt cgacgtgctg ccgcacggcc acctgtttgc
     1141 gctcgaccaa cggctgcgcc tccaccggct gcttcttctc gacgatcacc ggtttgggct
     1201 ctggctccgg ctctggcttg cgctcttcgg gcttcggagt gtctttctcc ggctccggct
     1261 tcttcggatc cggtgccttc ggcggggccg ttttgggctt ttcttcgggc tcggtggttt
     1321 cgtctttggg atccgcctcg gcatcttggg gcgtttgtgg gtcgcgctcg ggttccgggg
     1381 gcgtgacctg atcctccacc aaggacacct cgagcgtgtc cgtcgacgaa cgcacgtagc
     1441 tcctcacccc ttccacgaag cgtttgagct cgatgcgctc ctccatccaa tccgctgcgc
     1501 ggtgtgcccc gttacccccg agcaagtgga tcagcagcgc tgtggcgacc cagagaaata
     1561 aagggatgtg tgcgtcacgc ggcatggggg gagagcggac cttcgggcaa acctaacacc
     1621 cgctagatga cacagcaatt cacggctagg cgggctttat tggggtggca cgttcattat
     1681 gggagtttgc tgatcccggc gaagtgatcg gaaatttctc gtgcggtcca gacgccgtcc
     1741 tcggattcct tgaactttcc ggagctctcg acgaccttgt agacgctgag cctcgccccg
     1801 gccacggaca ggacggcgcc actcacgtcc cgtgccaaat cactcgccaa gaagaggtgc
     1861 gcgggagcaa cgtgctccgg cgtcaaggta ttctcgattt tcgcgaacat cggcaaatcc
     1921 tcggtcatgc gcgttttggc cacaggcgcc acggcgttta cgcggatgga ataccgttgc
     1981 agttcgatgc tcgccgtgcg cgtcaaaccg tagatgccag ccttggctgc cgcatagttg
     2041 gcttgaccga agttgccgag caaccccgag acgctcgtgg tgttgacgat gcttccgccc
     2101 cttccttgct ccttcatgac ctgggcggcg gcctttagac acaggaaact gccggtcagg
     2161 tggacccgga gcacggcctc gaaatcggcg agctccatgt tcaacagcgt cttgtcgcgc
     2221 aaaatgccgg cgttgttcac caacacgtcc agccccccaa gctgttcctg tgccaaacgc
     2281 acgagctcgt tggctccctc cgggctcgag atatcgtgct ggctggccag cgcggtgccg
     2341 ccctgggcac gaatctggtc gacgaccaac tcagccgcgc ccggatcatt cccgcggcca
     2401 tcccgagcgg cgccgacgtc gttgacgacg acgctcgctc cctctttggc gaacaacaat
     2461 gcggtggcgc gtccgatgcc cgcaccggca cccgtaatga tggcgacttt accttccaga
     2521 agcgtcattt gaggcagacg cttcctacca tcgaagctgg aacttggcct gagacttctg
     2581 caggagtggg ggcgttccga gcgtcgctcc ggttcggctc ggctgtttcc gatttccgtc
     2641 ccggtcttcg gcgtgacgca acacgagatc cacgcacaac acgagcccaa ccccgactgc
     2701 cgttccaatc acgtcccagg ccacgtcgtt ccaagacggg tctccatggc ctgacaaatc
     2761 ccagagctct ttggcggccc ccgccgaaag cgcgaggctc gctccggcta cgctgcgctg
     2821 ccaaggttcc tcccagatca ggcctgaaac ggcgtaacct cccgccgcga gccccatcga
     2881 agctccgaaa tggagtgtct tgtccgggcc aaagaaccga tcctcggcgc gcgctggaga
     2941 gacccaggcc aggccggcca ggcaaagacc gatcgatagc ccccgaccga acgcggggaa
     3001 gctcgagagc gtgcgacgcc tgcgagccct cgggcgctgg ctacggcccg cgtaattcaa
     3061 ccaagcgcct gcagtgcggt aaacgcccgc gagcagtgct atgaccgggc gctcatgacc
     3121 cgccgaattc tcgtcacgag cgcgttgccc tacgccaacg ggcacattca catcggccat
     3181 ctcgtcgagt acctccagac ggacatgtgg gtgcgcttcc agcgcctgcg tgggcatcgg
     3241 gtcatctaca tgtgtgccga tgacacgcac ggtacggcca tcatgatgcg cgcacaaaaa
     3301 gagggcaagc gcgaggaaga cgtgattgcc gaaatgagta gcgcgcatca gcgcgacttt
     3361 gccgaattcg aggtggcttt cgaccattac ggcagcacga actcggaggc cacgcgcaag
     3421 ctctgtcacg agatttgggc tagcctgcgt cgggcgtcgc tcgtgaaaga gcgcgaggtg
     3481 acccagctgt tcgacaccaa agccggcgtc tttttggcgg atcgcttcgt gaagggacgc
     3541 tgtccccgtt gcggcgctcc cgaccaatac ggcgactctt gtgataagtg cggcagcacc
     3601 tattcagcga cggagctgct cgagccggtg agcgcgttgt cgcagaccac cccggagatc
     3661 cgtaaggcat cgcacttgtt cgtcgagatc gaggctctgc acggctttct cgaggagttc
     3721 gtggccacgc cggagcgcct gcaagccgaa gttgcgaact acttgaaggg acacttcttg
     3781 agtgagcccc tgcgggactg ggacgtgtca cgtcccgcgc cgtacttcgg attcgagatc
     3841 ccggacgcgc ctgggcacta ctggtacgtt tggttcgatg cgccggtggg atatctcggc
     3901 gcgaccgcgg agtggtgtga aaagaacggt gagagcttcg atgcgtggtg gcgctccgcg
     3961 gacacggaag tgcatcactt catcggcaaa gatatcactt acttccacac gctattttgg
     4021 ccggcgatgc tgcaaaccgc ggggctcacc ttgcccactc gcgtacaggt gcacggcttc
     4081 ctgaccgtga acggcgagaa gatgtcgaaa tcgaagggca ccttcgtgct cgcccgcacg
     4141 tacctggacg ccttggatcc cgcgtatctg cgctactact acgcgagtaa gctcgggccg
     4201 cgcgtcgaag acctggatat cgcgctcgac gagttcgtga acaaggtgaa ctccgatttg
     4261 gtcggtaagg tcgtcaactt ggcgagccgc acggcgcgct tcgtggaggg gacgggtctc
     4321 tcgaaatcct atcccgatga cggcgggctg ttcgcggaag gcgcggccaa gggcgaggaa
     4381 attgccgccg cctacgaggt gagggacttc gcgaaagcca tgcgcatcgt catgggcctc
     4441 gcggaccgcg ccaacgagta cgtcgatcgg atggagccct ggaaactgaa aaaggatccc
     4501 gcccgcgggg cggagcttca ggccgtatgc agcgtcgtct tgaatctata tcggcaaatc
     4561 atcgtttatc tcgcgcccgt tctgcccaag ctcgcgcggg acagcgcagc gctgctgaac
     4621 gcgccgatta cccgcttcga ggacgccgcg gttccgctgt gtgggacccc cgtcgcggct
     4681 ttcagccacc tgatgcagcg agttgacccc aagaagctcg agagtgtgat cgagctcagc
     4741 cgtgaaaccc cagttatgtc caatcccagc tctgttcaaa ataccgaagc tcagcctgta
     4801 acctcgaccg ccacgagcac ggatgacggc tcggccttgg ccgcggagcc cttggctgcg
     4861 gaatgcagca tcgaggactt ccaaaaggtc gacttgcgca ttgcccgcat cgttgctgcg
     4921 gaacatgtgc cggaagccaa gaagctcctg aagctgacgg tgagcttggg cggcgaggat
     4981 cgacgcacca tctttgcagg catcaaagcg gcatatgcgc ccgaagcgct ggttgggcgc
     5041 ttggtggtcg tggtcgcgaa cttggcgccg cgcaaaatga agtttggcgt gagcgaggga
     5101 atggtcgcgg cggcgggctc gggcggcgat caagtcttct tgctctcccc agacgcgggc
     5161 gctcgccctg gccatcgttt gcattgaact gcggtgatcc gggcgcggcg gtgggcagtc
     5221 tgcatctgca acgcccaccg cgcgtcaggc ctcgtcactc gaaactgcag tcgttggagc
     5281 cgaacgactg agaacctcct tcagttggat acgctgctcg agtagctgcg cagatctttc
     5341 tgagctgcgc tgcggatcgc gcgctgcact ggcggcgccc aaccgaacag gatcccggtc
     5401 gccccgagcg ccattcggga ccagcgccac aggctgaagc tgtccttctg cttcacgatc
     5461 ttgccgtccc gcacttcgaa tagagacgca atgcgattgt gaactttgcg accggtctta
     5521 cggaacgtgt agcgcgcgtc ccactcgacc gttccgccgt tctccgtggg actcacgacg
     5581 aaggatactt ctagatcctg gccgcgctcg cagaacatcc gccacatccg ggtgaggagc
     5641 tcgtgatcca gctggccgaa gacgggatct tcgaatacgg cgtcttcggc gtaacagtcg
     5701 atcatgcctt gtgcgtctcg cctttgaaac gagccataca agtgggccaa gacttctcga
     5761 tttcgatcca agctcatcgt gtttccctca cgcgcttcgc tgcgctcccc ttggcgtcag
     5821 ctggggtcga actctaacgc aaaacgcttc gggaagggac cgacatattc gacgtgctct
     5881 ccgggctgga cccgtagctt gcgtaacacg agcacacgct gaattacctc cggcaggacc
     5941 acgcgattga tgtgctcacc gaagcagcgg tgttggccgt ggccaaagtg caagtaatgt
     6001 tctcgagggc gtccgggccg gaacacctct ggctcgtcca gcacgctctc atcgaacatg
     6061 gccgaaagcg tggacacgaa cacagtcgtg ccgctgggga tgaccacttc ccgctcggtg
     6121 cccgccccaa tcacggctcc gtttcggcaa aaccgaatca ggatgggatt gtgagggttg
     6181 aaccgcagcg cctcccagac gtacgcggct acgagctcgc ggtccccatc cagggccgct
     6241 tgctgcgccg cacgcagtgc tcccgggtgt tcgatcagct gctccagggc atgggtgatc
     6301 gctttggatt gcgtgtccac cgccccgacg atcacgcctc cgatgttgcg caccaacgtc
     6361 gcgtcgtcga tcggtgtgcc gtcctcgaag cgagcccctg cgagttgttg ctgtttgacg
     6421 agacggctca cgaagtcatc cgtcttcagc tgcccctcgc tcagctcgcg ttttctctgt
     6481 gcgactaagt tctcgaggta actgcgtagc tctgcgctcg agcgctccgc ctcctgtgct
     6541 acctcgacgt cgtccgtgaa gttcaggaag atctcccaaa agatcgagcg catccagcgc
     6601 gcgagcgtgg cccagtctgg accaggcgtg ccgaagtagt cccgcagcag gcgcagcggg
     6661 atcaagtgcg agaacccctc cactgcgtcg atcttgccct gcggctgtgc gcgtgtgacg
     6721 agctcgtcgg cgtgcgcgcc gacggaggca cggatcgcgg acatgtcttc cgcgtgcacg
     6781 gccgccctga tgaacgcggc ctctctccgg tattgcggcg tattctccat gccgagcacg
     6841 aacgcgcccg aggtgcgtcg catcttcgcg tcataaacct cggtgacgcc gaagtcgcga
     6901 tcgttcgaga gcacctcgag cacatcctgc cggagcgtta cgaacgcacg cccgtgcagc
     6961 acgatcgtgg gcgcgcaacg ccgcagcccc ttggttacca cccggagtac ctcgggtgac
     7021 ccgagccatc ccgtcagctt ggcacctacg tccgcaaacc agttttccaa gtcggtcatc
     7081 tgtggctctc catgcagcgc tcctttggct aggtaaccgg gcgccgttcg atcgttggtt
     7141 ccccactggc cggtgcgtag ttggggcgcg cgctcaagta ttcgcgagtg gcggcggcgc
     7201 cgcgctccat cagcgccagc ttctcctctt gggtaatgcc gaagttcgtg gtcggaatgc
     7261 caaggtcatc gatccgaatc acgcggcttc gattggccgg gctgtgtgcg aggtctacct
     7321 cttgctcgtt cagcatggcc ccaaacaaac actcgacgta atcgtgcatg gaacggagcg
     7381 ggcgcggggt cgcggccttg gtgctcatca agaaacccaa ggtctcgaag ttggcgccct
     7441 cagcggtgtc gaacgcctcc aacggcagat tccacagcac gccaccatcg acgtagacct
     7501 cgttttcgaa ctcacaggcc tcgaaaaata gcggaatcgc gatggagatc cgcaccgcat
     7561 ctaccaccgg catgtgccaa gcgtcgtcat cggggaacac gcggaccgag cgacgcgtca
     7621 ggttcgtccc gatcaccttg aggcgcttgg cgcccgcatc ccgcaagtcc gcgaagctcg
     7681 cgcgtggatt gttcaccttg cgcgcaatca gctcaccgag cgtttgggtg aggtattcgg
     7741 ccttgtgcca accgtagcgc ttcaaaactc ggaatggccc gcccaacaca ctcccgtcct
     7801 gtaaacggtt gaagtccaag tcgagcacgt gttggcggat ctccgcgggg ctatagtcga
     7861 ggctggccag cgtcgcgatg atcgagcccg aggaggtgcc cgcaaaggcc tccacttcgc
     7921 gatagagctg ggcctcttcc aaggccaaaa tgccgccgac gtaggcggcg ttccgcaccc
     7981 ccgtgccccc gaaaactagg ttcttgatgc gtggtttcat gctcgcgagt tcgtccaccg
     8041 attcattctt ccggcccggg cccggagtgc atcgtccgtt gcttcgtgcc tggcgatgtt
     8101 ccgcgcctgt attcgtcccc gagtccattc gtccccgagt ctattcgtcc ccgagtctat
     8161 attcggctgc gttgggtgac ggccgggtcc atgccggcgc gttcgcaaag tatcttcggg
     8221 ctcactgcgg acttcgtcaa ctcacaaaga catgggagcg aggcgctatc ctagttgcta
     8281 gcaacgctcg cgaatgacgc gagagcggct cggcttgcga agcgcgtcgt cggcatattc
     8341 ttgtagtcat ttgtttccga agtcggctcg cgcttgcgag tgatatcttc ggagtctatt
     8401 tccagggtgt gcgccagcag acactcacgg tggtgatgcc cgtgcgagga accctggagt
     8461 tgcgcgaggc attgcgggcg cgggccgcgc tgcacgagca attgcggcag ttactcgcgc
     8521 agcctgcact cggcatccat ttcgcgcgtt tggcgttttt ggatgagccg gaggcaggca
     8581 aacatccctc ctgcttcgtt ttcgaatcga atttcgatac caagatcgcc gacccgcgtg
     8641 aagcgatgca ggagcatttg cgcgcactgt gtgagctcgg gacgcccgcg atcgcagact
     8701 ggcttcatta ctccgagtcg aaactagaga gcgcgagcgg tcgagcgctc gccgactatt
     8761 tggaacgcca tctggtcgaa gccaccgcgt gttaccaggg gcacgtgcag cgggatctcg
     8821 gtcgtatccg cgccgagcaa gcgctgcgag acgtgatctt tacttatctc gaaggctgct
     8881 cgggccacgc gccgcacgtg ctgtatcgga agatccgcga acacgtgcgc gagcgggcta
     8941 cgcgagatcc tcgcctttcg gggctggact tcgacgcgcc cgcgccggcg cagccggatc
     9001 tggaggctcg tagccgggcc ctgaagtcgc ccgtatggcc gtggatcagg aatagcgacg
     9061 ttctgttgcc ggccgtgctg cggctcggtc gcgttgccgt gtggggaaag acggacagct
     9121 actacgagct gcgcaaacgc caggaagctt ggacggaccg agatcgcgag gtgttccgcg
     9181 atatttcgcg caccgaggat tttggagctc agaacgcgat tacgcacgtc gtgagcttgc
     9241 gcgatggaga cggtcgccta ggcgtcatgc gcacggctca ccgctacatc gcgcggttgg
     9301 cggagcgtta cttcaacgac gtcggtcaac tcggcggaat tccgagcatt catttcgcca
     9361 agtggttgct gttggatgga ggaaagcgac tgcttttctt gagcaactac gacagcagtt
     9421 gggagagcta cctgggggac ttcgtggacc aggcgtggat tggtttgaac ctggcatgga
     9481 gctgtaccgc cgagtatccg gcgacgcgcc atttggtatt agggggcgcg atggatgaag
     9541 agcgattcaa ggcttggggc cgctcctgcc agcgccccac cgagctgttt tacagcgcct
     9601 acccggacct cagcgtagcc gccatcaaca acaacaccgc catccgtgct gggcttcacg
     9661 cgccggcccc tccggatctc gatgcctggt tccggcgcct cacatgaact cgtctcgctc
     9721 gagaagccca tgagaacttc gctcgatctg ccggacattc agggtctaat cttgagcggc
     9781 tacggcgacc gcccggcggc gcgctacggc ctattcgaga tcgtggacgt ggcgcgtgcc
     9841 cggcgttggc tcgcgtcact cgtgccggag cttcaattcg gcgagtatcg cagatccgcc
     9901 cggcacgagc agcccttact gaagccgctc tgcgcgaacg ttgcattcac cgcggcgggt
     9961 ttccgcaccc tgggtctcac cgaaacggct ctggcggggt tcagtttggc ctttcaagaa
    10021 ggcatgaacg aaccagagcg cgcgcgtcgc ctgggtgacg acggggagag cgcgcccgaa
    10081 cgctgggagt gggggtatcc cgggcaagcc ttgcacggcg ttttggcggt ctttgcgggt
    10141 cgtgacgacg ctgaagaggc agagtgggag gtgatccgag ccttcttgga acgtcagctc
    10201 tgcgcggaac atggcgtcaa agccttgcgg gtgctggaca cgttggccac caatcgtgcg
    10261 agccggcgcg aacacttcgg gttccgcgac ggcatcacca atccacgcat cgcggggctc
    10321 tcgcgcggtc gtgaaaactt gattgagccg ggggaggtgc tgctcggcta caagaatggt
    10381 tacgggcact atccactctc gcccgaggtc cagagctcgg aagacccgga tggtcgcttg
    10441 ccagtttctt tggagctcga gacccgccgc gatttcgggc gcaacgggag ctatctggtg
    10501 ttccgtcagc tgcggcaaaa cgtgagcgcg ttttgggcct ggatgcagga aacgagcgcg
    10561 cgaatccctg acgctccgcg gggcgtacaa ggggcggtct ggctcgcgtc gaaggtaatt
    10621 ggacgttggc ccaacggcac tccgctcacg atctatcccg aagctccggg gccagacgcg
    10681 gccgcggaga acgacgagtt cgcgtttggt gaacatggcg atgcttacgg ctcgcggtgc
    10741 ccaatcgggt cccacatccg acgcaccaat ccgcgcgaca cggcgttacc ggtgccgcac
    10801 gatccggagt tgagcggaac acccgaagat tccggcacca agcgccggcg catgaagctt
    10861 tcggatctgc atcggattct cagacgcggt cgcctctacg gcgatccatg ccacgaccgc
    10921 ttcgacccca gcgagctgtg cagtgggaaa gacgacggca aggatcgcgg cattcacttc
    10981 ctgtgcttca atgcgaacct gagccggcag ttcgagttca tccaatcgaa ctgggtgaac
    11041 aatccttgct ttgccggctt gagtagcgat cccgatccgc tactgggcgc acggcgtgcc
    11101 tacccgttcg gggcaagtga cttcacgatt cagggctatc cgccccggcg agttcgcaat
    11161 ctgccgcggt tggtcgaggt caagggtggc gcctatttct tcatgccggg gcgcagcgcg
    11221 cttttgtacc ttagtcgcgc ctgactccac tcgcgtcgcg ggctttcacg gcgccacaca
    11281 ctcggtgttg aactcagcgc aacacaccca gcacgcggtc gcacgccggc cgcgcacgtt
    11341 tcccgagttt gaaactagcg ctcacccgcg ccgccgcgct gtcgagcagc gtggtgcgcg
    11401 cggccccgat tcgcgccaat gcgtcgccgg ctcggactcg cgttccgagc ggcgccaaaa
    11461 tctcgatccc ggcgcccgga tcgacggctt gatcggcgcg cgtccgtccc gccccgagct
    11521 ccaccgccgc tagaccgagc tctagcgcgt cgatttcggt caccacgcct gcttcactcg
    11581 ccttcagcag catctgcttg cgcgcttggg gcagccgctc ggggtgctcg atcacgctcg
    11641 cgtctccccc ttgctcgtgc accacccgag cgaagcactc gagcgctcgc ccgctcgcga
    11701 tcgctcctaa tagcgtgctc ctggcctgag ccgcgctcgt gctcacaccg cccagccgca
    11761 gcatctccgc tcccagcgcc agggtcagct cgcgagtatc gctcgggcca ccacctttta
    11821 gaacgtcgat tgcctcccga gtctcgagcg cgttacccac ggtcaggcca attggcgtgg
    11881 acatttccgt gagtaaggcc accactcgaa ttcccgacag gcggcccact cggatcagtg
    11941 ctttcgccaa gcgacgcgcg gaagcttcgt ccttcatgaa cgcgccgcgc ccgaccttca
    12001 cgtccagcac caagccttcg atcccctccg ccaatttctt cgacagaata ctggccacga
    12061 tcagcggaat cgattcgacg gtcgcggtca cgtcgcgcag cgcgtaaagc tttcggtcag
    12121 ccggggcaag ctcgtcggtt tgcccgatca ggcacgttcc gacgttgcgc acgagctgtt
    12181 cgaagcggcg tgcaggcagg tgcacgtcga agcccggaat ggcctccaat ttgtcgagcg
    12241 tgccgccggt gtgccccagg ccgcggcccg aaatcatcgg cacggccacg ccgcaggcgg
    12301 ccacgagcgg cgccaggcag atgctgatct tgtcgcccac acccccggtc gagtgcttgt
    12361 cgatcttggg tcgtttcacg gagcccaatt gcagcttctt gcctgagtca cgcagcacgt
    12421 gagtcagcgc cacggtctcg gcgtcgctca tgccgcgcag gtacacggcc atcaggaagg
    12481 cgctgatttg gtagtcggtc acgctgcctg cgagtaggcc ggagatcatc caacgcagct
    12541 cctccgggct cagctccccc ccgtctcgtt ttctgcgaat caattccacc gcgttcggca
    12601 cgcgggccac gctactcgaa accgggtccg ctgggtatct ggttcatggg tacggcgggc
    12661 gcagaccgca ccggggagtg cttgcggccc ggttccggag cttgcccggc attttgtatg
    12721 attgaggggt tggttcaaat cggatcggtc aaacaaagtc agctcgggaa ggcgcttcca
    12781 tcctgcgttc gaggcccgat ttggtagtct cggggctcgg agtggaagcc ttgactgaaa
    12841 cctctcgtgg ccgcccgagc ggcaagttta cgcagcatcg acggctggat cgtttgcgag
    12901 aaatcctcga gcgtcacccg cgcgggctct ccttgtacga gctggcggac gcgctcagcg
    12961 tgaccgtacg gacgatgcgc cgctacctga tcgagatgga gcgcgagtac gatttggagt
    13021 cgattcccac gcgcgggggt ggaccactgc tttggcgcat tcgccccggc gagatgccgc
    13081 gcaaggtaga gcttcgacga acccaagcgt acgcacttct cgccgcgcgt cgcctgttcg
    13141 agccgatgcg cggctctgcg ctgttcgaag aaatcgactt ggccatcggc aagctgctct
    13201 cgttcgcgca gcgtcccggg cgcggcccta acgcgggtct ggcggatgcg cgcttggaga
    13261 atcggttttt gtacttgccg cacgcgccca agaattacac cgaaaagacg gaggaactgg
    13321 acgacttgtt tcaagcggtc tccgatctgc gcccgctcac gatgacttat cgcagcgcgg
    13381 gcaaggcgga agaggagcgc atcaccattc acccctacgc actcgtgttg catcgggact
    13441 cgatttactg cgtcgccttt cacgtcggcc gaggtgaggt gcgcacgttc gttctcgatc
    13501 gaatgcgcga cacggagtgc gccacgacgg agcgcttcga tttgcccgag gcgttcgcgg
    13561 tggacgagta ttttcagggc gagttcggga tctggaaggg cagcacgccc cagcgcgtag
    13621 tgatcgagtt cgacgcaacc gcggccgagt acgtgcgcat gcgcaaagtg catgcaactc
    13681 agaagttgtc gcaaattcag ggcgggggcg tgcgcttgac gatgacggtg ggcaacctga
    13741 atcccgtcgt gagttgggtg ctcgaatggg ggcaccgcgc gcgcgtcatc tcgccgccgg
    13801 agcttctgga tcgagttcgc gaggaactcg gccgcgcgtt gggcaactac agttgagttg
    13861 gcctcgcgag ctggcgttcg aggcggcgtg gagaggaacc gcatgagctc gtccgcttga
    13921 cgaggcgccg acgggctcgg tgcactccgc aaggcgcgat agcaacccgc agccgaaact
    13981 agcgtgcggg tgcgcgctgc gcgctgtact ggcggtaata cgcaagcacg cggctgacgt
    14041 agtcgcgcgt ctccggataa ggagggatgc cgccatgccg catgacggcg ccttcgcctg
    14101 cgttgtatcc ggcgagggtc aactggatat ccccgctgaa caggttcgcg agcactcgta
    14161 agtagcgcgt cccacccagg atgttctccc gtggatcgaa gatatccgtt accaacatgc
    14221 gctcggcggt ttccggaatc agctgcatca agccgcgggc gttggcgggg gaaaccgcgc
    14281 gcggatcgaa atcactctcg accttgatca cggcgcgcac gagctcttct gggatttggt
    14341 acagcgccgc ggcttcgcgg atgtgcgagt cgtagcggga gaatcgctcg ctcgagctat
    14401 cactcggcat cgcgacccgg gtccgatcct tcggagttcg tacgtacagc ttgccgctct
    14461 tggaagagtt cgagaagctg atcactccgt cgggacccac cgaacgatag atatccgcga
    14521 aagcagcgtc gctcgtgagg gcgagaaaca gcccgagcgt cagagcccag ccggtggccc
    14581 gatgcgaccc tgaacgatgg ggacggggga aggacatgcg ttggaaggag cgaggcactc
    14641 ggatcaatct taccgagtca ccggctgggc taaccagaaa acctcgctcc cgagaagttt
    14701 gttcccactt cgccaccaaa tctgcgccgg tgtgcgggaa gcccagcgtg ggtcgcgtcg
    14761 cgggctccga gcaacgcagg ctcagcgcct gcgccgagct actcgcggca ccgaacccgt
    14821 tggccgagcc ggctcggaat gcgccgttcg cgcaccgagc cctttacgcc cgatcagtcg
    14881 tcaccggtga gataggtgta cgagcgaagc gaatcctcgt agtggcgcat caaggtgcga
    14941 agttggtcgt tggtcagccg gcctgcactc acggcgcgtt cagcttgccg acgtacggcg
    15001 tccaccatgc tgtctggttg gtattccacg taacgcaaga cctcggacac gctatcgccc
    15061 ttgatcacgt gacggacgtg gtacccgccg tcttcggtca gcgcgacgtg caccgcgtga
    15121 gtatcgccga ataggttgtg cagatcgccc aggatctcct ggtatgcgcc gttcaagaac
    15181 atcccgaggt aatagggttc gctgttgtcc ttcagcgtgt gaacatcgag cgagctccgg
    15241 acgtcttcga cgtcgatgaa gcgatcgatc atgccgtccg agtcgcacgt cagatccgcc
    15301 aaggtcgccg agaccgtggg ttcctcgtcc agccggtgaa tgggcatgat cgggaagagg
    15361 tgatcgatgg cccagatgtc cggcgctgac tggaatagag agaagttgca gtagtagatc
    15421 gcgctcagcg tgcgatcgag gtgcttcagc tcttcgggca cgaagttcag gcgtcgcaaa
    15481 gtctgctgca gcttctcgca gcagttccaa tacaagcgct ccacctgtgc gcgctcacgc
    15541 agggtcaagt aaccatactt gaagaggctc tgggcctctt ctttggcctg actcgcgtcg
    15601 tggtagctct cttgcacgtt cttcggctga atgccgtgat acgtctcgta gagctccttg
    15661 aggaggcggt gggcttcggg cttcggctct agtggctcac cgaaacgcac ctcgttgcgc
    15721 cccaccacct cgaagaccag caccgagtgg tgcgcggcga tcgcgcgacc gctctcactc
    15781 accagggtag gcaccggcag atcggccttg gagcaggcag cctggatgtg cgccacgacg
    15841 tcggcggcat actcttcgag ctggtagttc ttcgaggcgt ggaagtccgt cttggatccg
    15901 tcgtagtcga ccgccaagcc accacccacg tccaggtagc ccatgcggca acccatcttc
    15961 gcgagctcga cgtagacgtt cgaggcctcc tgcatcgcgt tcttgatcgg aatgatgctg
    16021 gagatttggc tgccgatgtg gaagtggagc agctgcagcg aatcgagcat gttgcgctcg
    16081 gtcaggcgat ccaccacctc gatcatttcc gaggtgttca gcccgaactt ggcgcggtca
    16141 cccgccgaat cagcccagcg ccccacgccg cgcgtcgtca gcttggcgcg cacgccgagc
    16201 agcggccgaa tgccgagctt ctcggaggcg cgaaagacca gctcgagctc ttcgaggcgc
    16261 tcgagcacca cgatcacggt tttgtcgaac ttctgcgcga gcagcgcggt ctcgatgtac
    16321 tgccggtctt tgtagccgtt gcagatgatc aagccaccca cgtcgctcat gcaggcgagc
    16381 gcgattagga gctccggctt cgagcccgcc tccagcccga agttccactt ccggccgacc
    16441 tcgacgatct cttccaccag gtgtcgctgc tggttcacct tgaccgggaa caccccgcgg
    16501 taggtgccct ggtaatcgta ctcggcgatc gcgcgagcga acgcttcgtt gatgcgcttg
    16561 atgcgatctc cgacgatgtc cggaaagcgg accagcagcg gcgcttccaa acctctcgca
    16621 gtcagctcct gcgtcagctc gtacaagtcg atgtgctgct ggcgttcggg gtccggggtg
    16681 acctgcacgt gcccgcgcgc attgacttga aagtaaggag ctccccagcc atggatctga
    16741 tagcgctggg ccgcgcgatc gagggtccac tcgggggaac cgttctgctt ctcgacagtg
    16801 gccgagcgac gtgaaacgtc gagggcggca tccagggaaa tggcagagag cttggcgccg
    16861 ttcatggtga tgtgcgcccg gaatatagct gacttttcga gcggctcgcg ccagatctca
    16921 cgaaaatagt gtgtcgaatt cgcgtcgccc gcgtcccacg atcccccgcc gctccggtgg
    16981 caccagagct gggcgctacg gcgcgaacca gacgtgaggc agcgcgttcc ggtgactcag
    17041 agccaggatg tccatcgaga gggctacggt gacatggacc aaaaagcccg cgtagatgct
    17101 gcgagtacgc atcgagagtg agcccaacac cacacctgcc acgattgccc cgttcgcctc
    17161 caagtacggt ttcccgtaat ggatcatgca gtaggggagg gccatggcga agatggccga
    17221 ggcccccagc gtgcggcgta gggcggacag catgaagcct cggaagaaca cctccagcgc
    17281 gaagaactgg acgatgtaca tggcttccca ggcgaaccag tccgcgtagc tgcgggagct
    17341 cagcttgtag aaggggtaat agctgccgaa gtccggctcg cgcgcgacga tgaacatggc
    17401 gggcacgacc accgccaggc aaagcccata gatccagacg tgctcacgga gaccgcgcag
    17461 ccgcagcccc atgtcgagca ggctgtcttt gggaaacagc agcttccaaa gcgggagagg
    17521 aaagagaacg tacccaatca cgcgcgcgct ggaccaccag agatatcccc agagctcgtc
    17581 gtacttcgcg aagcgatgcg cgccctccgg tccccagttc acggcgctca gcgcggggcg
    17641 gattacctga tcgtagaagg aacgcccgcc gtaatattcc tgcaccgtga gcacgatggc
    17701 cgtgatcacg aaacaggcgg cagggcggta gtccatctgc gggcccgcca aggcgcgttc
    17761 cgcggttgcc tcgcgattga cctcctccca ggtgcgccga aaaaaccaga acacgatcgg
    17821 cgtcaaggcc gcgaggattg gaatcggcag cagcgccttc agcggtggat agccgagcaa
    17881 cgcggagaaa taggaagcag gctcgattcg ctccaccggc aacagcgaag tcgtcacctc
    17941 tggcgtgagg ggctttgcgg gcccgaatcc gagcccgccg ctccaagcgc tgaatagcgc
    18001 gtgcatcgag ggtttcagcc agccgcaagc gcggcgccgc gggtcaagcg cgccagcgtc
    18061 gcctcgcggc cgagcacggc catcacgtca taaagaggtg gactcgcgct gcgtccggtc
    18121 agcgccacgc gggccgcttg cgcaaactct ttaaggctga tcttctcggc ctcggcgtac
    18181 gccgcaccac acgcctcgag cgcagcggga gaccaatcgg caacgcttct caaggcctgc
    18241 tccaggccgc gcaggcgcgg cgccgactcc ggcgtcagga atttctgctt cgccttttca
    18301 tccaggttgg gctcggcgcg gaagtagaaa tccatatccg tagccgcgtc cgcgagggtt
    18361 cgtgctcgct ctcgaatgcc cggaatcgcc gcacgcaggc gcgcgggatc gacggcctga
    18421 tgcccctgcg ccgccaagaa cggctgaacc agctgctcgt actcggcgag gggggtgagt
    18481 tcatccctct tcaagtgttc gaacgccacg tcggcgaact tccgctcgtc gaagcgaccg
    18541 tcgctcttgt tgacatggtc ccaatcgaag agctgcacga gctcgctgcg actgaagatc
    18601 tcctgatcac cgtgagacca gccgaagcgc accagatagt tgagcacgcc catcggggtg
    18661 aagccgcgct cccggtagtc ctgcaccgca acggaggcgt ggcgcttgct gagcttctcg
    18721 cccttcgagt tgagcatcat cggcaggtgc gcgaactccg gcggttccca accgaaagcg
    18781 cgatagagca gcacctgctg aggggtatta ccgatgtgat cacggccccg cgccacgagc
    18841 gtgatatcca tggcgtggtc gtccacgacc gcggctaggt tgtagagcgg ataaccgtcg
    18901 gtccgaacca gcacgaaatc ctgctgcgcg gcgttgggtg tgctgatggc gccgaacacc
    18961 ttgtccacga aatcggtgct cccgtcgcgg ggggtcttga agcgcacgac gtgcggcaaa
    19021 tcagggcgat cggtgcgctc tcggcaagtg cccggataga cgaaggccgc tttcggattc
    19081 ttctgcttga gctcctcgcg ggcgcggtcg agctcctctt tggtgcagta gcagcgatag
    19141 gccttacctt cgcggatgag gccttccacg gcttcgcgat aggcggcctt gcgctcgctc
    19201 tgaaagtacg aggtataggg gccgcccact tctggacctt catcccagtc caggccgagc
    19261 cacttgagcg catcgaggat ggcgcgcacg ctatccaggc tgctgcgctc ttggtcggtg
    19321 tcctcgacgc gcaaaacgaa ggtgccgccg gttttgcggg cccacaacca gttgaagagc
    19381 gccgtgcggg cgccaccgat atgcaagtac ccactaggcg aaggagcgaa gcgcaatctc
    19441 ggcttcatgt tgccgcccgc cgactaccac gcgggctgcg atgcgtcacc cgacgagtgg
    19501 ttcgtggtgt gcgtttctct cttgtcgggc gtgagtccgg cggccagcgg cgactgtgcg
    19561 gcgggcggta ggagctccgg gaccgcgata ttcctgcccg acgcagattc tgatccattc
    19621 gcagttgctc cgctggccgt ccgttgctag aaggccggcc ctccgtcggc cccttctcgc
    19681 gcggcctgga gtcgaacgat gaaaaagtcc cccctcagcg ccgtcaaaga gcgtttcaac
    19741 tccaaggacg agctcgtagc agccgttcag aagctggcca ctgaagacat gtggctcgac
    19801 cgcgtcagct ccaccaaggg cctcggccgt gtcgcgaaca gcaaactcct tcacctgcac
    19861 gacgtgctct cggacgccaa gtcgcgtttc ggaagccgcg ccaagctgat cgacgccatc
    19921 ctcaccctgc agaagcgggt caaggacgca ggctacaaga gccggctcga aggttacgcg
    19981 ctgccgcgtc tgctcgacct gcacggcacc ttgagccgca ccgaaaagcg cgctcaagcc
    20041 aaggccgccg gcaagaaagc tccgaagaag gccgctgctc ccgtcgctcc gcctgcggca
    20101 ccggccaaga aagctgcggc caagagcgtt gcgccgaaag ctgcacccgc ggccaaggcc
    20161 gccgccgcga agcccgccgc ggccgcgaag cctgctgcca agcctgcgaa agccgcaaag
    20221 aagaagggct gagcccttcg ttcgggcccc aagcccgcac ggtccgccgc aagcaagacc
    20281 atgcccaagc gccggagctc tcgtagcttc ggcgcttgcg tttgggcagt cggttcaatc
    20341 attcaacgcc gtgctacggc cgcacgcttg cggccgttcg ctacccgcgg cccaacccat
    20401 gggccccagg cgatcagcgc aggccttttt cggcgatgaa ctgattcgtg aactcggcgc
    20461 tcttggagta actcttctcc aagtcggcga tgatcttctt ctcgagcaga ccgccgactc
    20521 cgaaaatctt cgcggtcacg ctgcctttga cggtgcgctt gcagcgctcc ggggagctcg
    20581 ggtcagctac cgtgatcatc tcgagtttga cctggatctt gtccgctaga cgattcggca
    20641 cgacctcgac ggtgtagcgg tgcgctgcgc ggtccagcac gccgcgctct tggtaaccag
    20701 cattgtcccc gatcaccgcc ttgagcgccg cgggaacctc acccatgggc ggtgcggctt
    20761 cgaccacgcg atgaagcttt tgatcgcgct cctcgcgcga agcctcgcgc caggccgaga
    20821 acttgagccg ctccaagaac aacttgcggt tgtattcctc gtcgagaaat accttcgacc
    20881 agaaggtatc ctcgctgcag ttgaaggtgt gctcgacacg aaagtccggc atggcgggca
    20941 gcttaccaca gtgattttcg gcctcggcgg tgaagcaggg ctcgactaca cttttgcgcg
    21001 cccccatgac cgtcgcccgt gacaccgccg ttctggcccc gctacgtcgc cgaacctggt
    21061 tggcacaagc gcgcgtcatt ctccagaagg acgtggcgat cgaggtcgcg acgggagagg
    21121 tcgtgatcac ctcgggcttt ttcgctctgc tagtggcgat cgtcgcgtcg ctctcgtttt
    21181 acggcgggcc tgccatgcaa cgcacggtgg gagcgggcgt gatttgggtg agcttgacct
    21241 tctcggcggt gctcgcgctc gggaagacgt ggcaacgtga gcgggacgaa tctgcgctcg
    21301 agggtctgct ggtcgcgccg ctctcgcgca gcgccatctt tgcgggcaag gcgctcgggg
    21361 tgctggtgtt tttgtgggtg atcgccggcc tcttgatccc gctcacggcg ctgctcttct
    21421 cgctagattt gctcgaggtg gggcccggtc tattcttgat tgcggccgcg gctacgcccg
    21481 ggctcgccgc gagcgggacg ctgttcggtg ccatgaccgt tcgtacgcag gcgcgtgatc
    21541 tgttgctcgc ggtcgtcttg ttcccattgc tcacccccac tctgctctgc gcggtggcgg
    21601 ccactcgtga gctgttcgcg ggaacgccgg tgggagaact cgcggactat ttcaagctgc
    21661 tgttggtgtt cgatgtcgtg ttcgtgagcg gcggcttgtc actgttcggt acgctgatcg
    21721 aacgttgact ccgttgtatt ctgcacgcgc cgcgcgggct acccgcgcaa ggcgttcccc
    21781 agcgtgcgac ccgcctcgta gccctggtgc tccgcagctt cggctgctcc cgcggccgcg
    21841 ccgatacagt acacgccgga cgcgacgcag tgatcgtgct ctcgggcttc gggcacaaag
    21901 cccaagtccg ggttgaactg cacgcgggcc cccgcttgca cggccagctc gaagctcggt
    21961 gcgggaggtc cgtcgcacag cacggcatct accttcagac gccgcccctc atcgtgctca
    22021 cggaaacgca aaccgctgac ccgcgttctg ccctcggcgc ccaagacggt ttcgaggttc
    22081 tgcaagcgca ccgcgagttg ggacccgagc tcacgcgcgg cgcaatctgc gaccgggcca
    22141 agccccagca ccgcgacccg ttcgcccggc gatatcccgg aacgcagcaa ctctagaccc
    22201 gcccgcgcgc taaagatgcc cggcaggtcg gagttggcga actcggggat cacggcgtgg
    22261 cttccgcacg cgaggatcaa acagcgagtt cgaacgcgca gcattccacc gggacgcgcc
    22321 accagcactt cgaatgttct gggcgattct ccggctcgtg gcgcgtccgc gtgcccctca
    22381 tcgagcgctc gttctggggc aggctcgtag attccaatcg cggtcgcgcg ggtgcgcagc
    22441 tgggcacccg agtttcgcgc gtcttccacg cgccgaacac tctcggcggg gttcaggagc
    22501 cgttcgacac ctccgagctc cggagccatt tcgacgagca gcgccttttc tccaagcccc
    22561 cgcgccgcgc tgagtccggc gactccaccg ccgatgatca gcgcgggcca gctttcatcg
    22621 ctgctctcga gcccgagctc cgctgggagg tcgggcagat tgccgagtcc ggcgatacgc
    22681 cgggccaggc gcgtgaccaa cgccgacacc ccccggacac ccgcaaatag gcggtggtga
    22741 tcgatcccgt gcggaaagag gaagtcgttg gcctgcagca gatcgacctc gcgggagccc
    22801 agcacgttct gcgtctgtag aacctcccct ccgcacactc ggtgctgaca ggccgcaacg
    22861 ttgggaaccc catccacccg caacaaacag ccgtcgcact cgccggttag acaaaagggc
    22921 ccgcggggcc gatgcaactt ggggctgcgc gataaaacca gacgatcggc ggcaatcagc
    22981 gcatgggcca gtgattcgcc cgggcgggcg tgcagggcgt ggccgtcgtg cgtgatcacg
    23041 actgaatcct cgagcgcgct ctgacggaag ctcaccgctc gacgctatcg cgattcgggc
    23101 gcctcggacc cgaaaattgc ggtggacctc ctgccaagcg agctcttgcc aagcgagctc
    23161 tttccaattg aattctggcg cgaatggcgg gaaaagactc gcaaggctag cttgatgcgc
    23221 gtagcagtat tcggcggcag cttcgaccca ccgcacgtag cgcacgtact actggcgacc
    23281 tacgctctgt cggtcggtgg cttcgatcgc gtgctggtcg tgccggtgtt tgctcacgcc
    23341 ttcagcaagt cgctatcacc tttcgcgcag cgattgcgca tgtgccagtt ggcgtttgcg
    23401 cctctgcgag ccgtgcaggt gagtgacatc gaagcgacct tgcccactcc gagcttcacc
    23461 ctgagcacat tgcaggcgat ccggcgcgag ttgccggatg catctctcaa tttggtgatc
    23521 ggtgcggacg ctctgtccga gacttcgaag tggcatgcgt tcgatgaggt ggctcggatt
    23581 gcccctcctt tcgcggtggg ccgagagggc tacgacgcga gcggggcgag ccactttgcg
    23641 ctgccccgag tgtcttccac tcaggtgcgt aagttgttga cgtcgagcga tcgtagcgcg
    23701 cagcgcgcgg agttgagtga gctgctgcca cggtccgtgc tcgagtacat cgagcagcac
    23761 gggctttatc cgccgcctcc gtagctggca gcgccggagc tggcggagca gcgttgaagc
    23821 accgcctcat gtctggaacg ctcctaccgt tcgtggtctc gtgaacgggg cctgaacagt
    23881 gctaggctgc gcgccgtgga tctgcgcacc cgcacgtcct tgttttgcgg agttctggcg
    23941 ctcgccattg cggcgtcgat actcctgcgc ggcaggcctc gacgcgcaca gattctgttc
    24001 gctgcactcg ccggcgatat gggcctctgg tacctcgcgc agtggcagta tcacagtggg
    24061 cgagcggact tttgggagcg ctcgacggcg gtgctcgccg tgcttctgcc gcagtttgcg
    24121 ctgcacttat tcgacgccat cgtgccgcgc gtgggccgtc gatcggcgtt gctacgcgtc
    24181 gcggggctac tgtgcgtgcc gatgctcggc ttggtgctga cggaacatcg gcacggcctg
    24241 gtgcgcagcg cggtaattgt ctacgtcttc gggttgatcg cggcgggtct ctgggctcta
    24301 gcgcaacgcg gtgagcacag cggctctcga gcgacacagc gccgagtacg cttgctggtc
    24361 ttgatcgggg ccctggccgc ggccttcagc ttcgcggact tcttgtggtt catcggcgcg
    24421 ccgttgccgc cagtcagcgc ggtgctctcg atagtattcc tgtttgccct ttccgagtcg
    24481 ctgatccgcg cgcggctggt ggacctgtac gatttgttgg ggcaaatcct ggtgtccacc
    24541 gcgctcgcgt ttttgctggc cggtatcttc tatgtgttcg tggtgttgtt gggcggtttc
    24601 gacaccatgt atttgggtgc gatcctggcc gccatcgtga tcctggtgtt gttcgagccc
    24661 ctgcgtgaga aggcggagag ctacacccat aaggtgttct tcagagaacg tgtcgatctg
    24721 gagcgtgcgg tgacacgcgc tcggcgcgcc ttgattcacg tattggacgt aaacgagatg
    24781 cagcaggtcg tgctctcggc cctcgaagcg tcgcgtcggg cgacaggggc ggccttgtac
    24841 actcgtcacc cgagcgggcg tgatttcgag ctcggggcga gcttcggccc ggtggcaccg
    24901 ccccgcatcg aagtggcgac cgcgcacccg ctcttcgagc gcctggacag cagcgcgtcg
    24961 atcgcgttcg aggaggtggc ccagcgtgtg gtcgaacggc gacgtgtcgg cctaatccgc
    25021 gaggccgagg ccgacgagcg cttgctcgcg gcggccgggg tgctcggctc ctttgcgcag
    25081 gggctttgcc tcggggtata cggagagcgg catgagctgc tcgcgatctt gctcgtcgtc
    25141 gatgatcgag tgcgagacgc tttttcatcg gatgaggtcg ggttgctgga ggcgctcgcg
    25201 gttcagatgg gtgtggtgat cgaaaactcc cgacagcatc ggcgcttgca agagcaagat
    25261 cggctcgccg cgctcgggca aatggcggcc gggctggcgc acgaggtgaa gaacccgttg
    25321 ggcgccatct aaggtgcggc ccaactgctg ggagagcccg gcggcaaact agacgtgaac
    25381 gaacgtgagt tcgtcggtat catcctcgag gaagtggagc ggctcgatcg cgtggttggt
    25441 tccgtgctca actacgcgcg tccatccaag ggcgacgtcg gcgcggtgga cgtgaacgcg
    25501 gtcgttcgtc gcacgctgac cgtgctggca tccaatcccg ttcagggttg tgagatcgtc
    25561 ag
//
LOCUS       DASTKU010000388        11841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_964582, whole genome shotgun sequence.
ACCESSION   DASTKU010000388 DASTKU010000000
VERSION     DASTKU010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11841
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964582"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFQ61_13390"
     CDS             complement(<1..204)
                     /locus_tag="VFQ61_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955499.1"
                     /translation="MTSTANSSSVSVSAAPTSASPVEIRVQRTGSVAVDMPSYQSSGA
                     AGLDLEAALLTSVTLRPGERRLIP"
     gene            complement(296..2923)
                     /locus_tag="VFQ61_13395"
     CDS             complement(296..2923)
                     /locus_tag="VFQ61_13395"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9955500.1"
                     /translation="MLGRTASASEPLVGRDEELSLLRRAYHDVAEGVGSRIVTLVGPQ
                     GIGKRRLISELTQELGAGPLPARVCEGRPAAQSAAYGMFASLLRSRFEIPEGTESDVA
                     RTRLHAQVSAVLGDRRVGDVCYFLGELMGLAFAESPLTRAVADDPSQARLLRRAVVRS
                     FIEADAAKSCVCLVLYDLHATDEDSLELLAYLLENLRAKILVLGTARPEFLSRHPEWS
                     TLGGSRHALLELGPLSEDDSLTILQRLLAACHGGPPPRLLEAAISMAGGTPGHLVHMV
                     RVFRDAEVLVGNELEGFRVDLDRLASARLPLTVADAVELRILALAPHERRLLEYAAAL
                     GSVFWLNALVALTRIDRDPPEFWTRESLEDRERVKRTLTDLEQRDYVLRLPDSAFAGE
                     SEFVFKHNLERESLGRLTPAATTRRYHQTIADWLAQKETVRSQEEYTAMLARHLENAG
                     SLTRAAFTYIESGHKAREHYAAKRADEYYRKGLELLGEDDARIRIDALHNHGDVLVLL
                     GRTDEAFAAFREMRAIAYRLGLPTKGGAAHNRIGRLYRDTGSLGQAQQHFEAALSLFE
                     SGGDQRGVASCHDDIGRLLWMRGEYAAALDEMRHALEMRKTLGDGRSIALSLNNIGLV
                     WRDFGRVTEAHEALEAALKIRRELNDPLGIVESLNDLGQLAFDQNEPERALALFQEAR
                     EVASEVSEHNRIAIVLTNMGAAHLRLTDSDSAIRVLVQAEELCDELGDKLHLAEAKRM
                     LSKAYLVRGDLKRAREAIKRAVDLFGQVRSKPHLAMALRTLGEVTAAGAWGEGHEGKA
                     VDYFLRSIAICQEIGNEFEVAKSYRAFSSYVEGSPHYRHNPDIVREAKKLSAMADEIF
                     ARHRVEPSRADASTRVDRA"
     gene            3295..4143
                     /gene="accD"
                     /locus_tag="VFQ61_13400"
     CDS             3295..4143
                     /gene="accD"
                     /locus_tag="VFQ61_13400"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET9955501.1"
                     /translation="MTLPERTPAQSTSAEKRSVGKGLFSKCAGCGVVFTAEEFSSRWN
                     VCSSCGRHHPMGRAQWRELLLDDAALDVWDDHIAPIDPLKFEDGRPYRERVERAKRAT
                     STSEAVEIGLARIEGQRIGYGCFVFGFMGGSMGSVVGERITRLFERAASERLPVLLLH
                     ASGGARMQEGILSLMQMAKTVSALRRFRECRKPYISLMLNPTTGGVAASTAFLGDINL
                     VEPNALVGFAGPRVIQNTLRTQLPEGFQSSDFLLSHGMVDAIVPRAELRGKLVQLLRH
                     LGGDVA"
     gene            4140..5372
                     /locus_tag="VFQ61_13405"
     CDS             4140..5372
                     /locus_tag="VFQ61_13405"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HET9955502.1"
                     /translation="MTLDLKKTLERLYALGSRGAKLGIERMQAACARFGNPERAFTSV
                     HVAGTNGKGTVVAFVSAMGRAAGLRVGTYTSPHLHRFAERITIDGTPIDDAVLVPLLN
                     RVMDAAPDLTFFEVATLAAFIAFRDAGVQLAVLEVGLGGRLDATNVIPAPRVAAITRV
                     AFDHMAELGDTLTKIATEKAGIIKPGSAVVLGKLHPDGRAVMERRAAEVGARLVPLGS
                     PEPIPGAPLAYPRVALLGSNLAVAVTVGRELGWAPEVLAQGVENTVWPGRNELLHRNG
                     MDLTLLDCAHNPDGAVALSHALDPTVLADIESRREIALVFGALSTKNWRAMLRRLEQV
                     AGQRVYVAPPVAKAADPRQMLEVASGEVALSVEEALTRARELVGPKGVVVVAGSIFLV
                     GAARALLLNLPTDPPIAL"
     gene            5409..6776
                     /gene="glmM"
                     /locus_tag="VFQ61_13410"
     CDS             5409..6776
                     /gene="glmM"
                     /locus_tag="VFQ61_13410"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET9955503.1"
                     /translation="MTQRKLFGTDGIRGRANVAPMTPELALRLGRALAYVAGRGKSRA
                     PRVLIGKDTRLSGYMLETALAAGICAMGGRVMLCGPIPTPAVANLAQSMRADAGLVIS
                     ASHNPYADNGIKIFGPDGFKLPDSEEAEIEALLEDPRLDKLGRTGSKVGRAERIDDAM
                     GRYIVYTKNTFPRELSLHGIRVVVDAAHGAAYKVAPHVFEELGAEVVSLGVRPNGTNI
                     NKNCGALHPEHVAHEVKRKHAQLGIALDGDADRVIFVDEKGNIADGDSIMALCGLRML
                     TEKRLSKRTLVTTVMSNMGLEHALARAGGKLVRTAVGDRYVVEEMRRHGYNFGGEQSG
                     HLVFAEHATTGDGIVAALQVLAIILREGKPLSELCQVMERVPQVLENVSLPARRPIQE
                     MRELTRRIEQAERKLGKNGRVLVRWSGTEPKLRLMLEGPEPSILRSLVAEMKNAAKKD
                     LAASA"
     gene            complement(6907..8208)
                     /locus_tag="VFQ61_13415"
     CDS             complement(6907..8208)
                     /locus_tag="VFQ61_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955504.1"
                     /translation="MSASTGRKKTGFVIAWVMLLSAPALLTQCGKVPGANNLPSVPGV
                     PDVGGACPANIADAAAIMNANFGLEGELEGKVKAALAAGANLQKIAVDVEGEVTTACS
                     NLAKDLGATDEQLKPAEAGPGKGAEAACNVASKLIAETKAKAQGKVMVTAKPPQCAAS
                     MSAMADCAAKCDAKIKPGEAKVQCEGGKLSGKCEGKCEGECTVEGAAKCEGTCAANCT
                     GECKADFSGKCDGTCNGKCDGKDSKGKCAGTCDGTCAGKASGTCGGECKGDCSAGCTM
                     KAKAECNGTCSGGCSVEFKEPKCSGEVKPPEMSAECKANCDAEVKAKLECTPGHVAVK
                     AEGSADAQAAAKLKAALEKNLPVLLKVSVGMKDKLGSLAGNVQASLEGVKGAVTGGGA
                     AALKVGTCLAASIEAQAKASVSIKVSVQASASASASAKGST"
     gene            complement(8509..9003)
                     /locus_tag="VFQ61_13420"
     CDS             complement(8509..9003)
                     /locus_tag="VFQ61_13420"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET9955505.1"
                     /translation="MKLPIVLVPHPHVRVDTNVLGGSPHVVGSRVPVRRLWAFYRGGT
                     SVDKILKRFPQLGPAKVFDALAFALDNPEVIEADIRQEQELLELVTHSVRKKPGNDSQ
                     MELPFAGNIESVSRADASEAAKHGHSGEREPSSADREVSNRAAKTEPSELKSRSRKKP
                     ISER"
     gene            complement(9000..10259)
                     /gene="mltG"
                     /locus_tag="VFQ61_13425"
     CDS             complement(9000..10259)
                     /gene="mltG"
                     /locus_tag="VFQ61_13425"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET9955506.1"
                     /translation="MTAGSERPGSGKPGSSGGSNARPPRGGSSGEAARGGRSRRSRPP
                     SSRSLAPPGPRPRRRWTKARRLFWGGALCLVVAALSCAGAILVWATREAPGARAPVTM
                     SFTGTEDLAETGKRLGDRGLVTNAFLYSLYLEWFAGGLKVPKGAHLLETGLSPRELTQ
                     RIGRLPSRPRVKVAIPEGYTQLQIAERLQARGVCTAHEFLEAATDSALMAELTISGPS
                     AEGYLFPATYELFVNSEAAFTVRLMVAETRRRLARLSERSGRGIERVASERNWTEREV
                     LTLASIVEREAAVGEERPLIASVFYNRLDDSSFRPARMLQSDPTAAYGCGVARVPAQS
                     CQAFTGKVTPELLRDASNPYNTYRHPGLPPGPIANPGEDAILAVLNPARTDYLYFVAD
                     GRGRHRFSRSFEDHRRAIESPTLNSTP"
     gene            complement(10256..10678)
                     /gene="ruvX"
                     /locus_tag="VFQ61_13430"
     CDS             complement(10256..10678)
                     /gene="ruvX"
                     /locus_tag="VFQ61_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET9955507.1"
                     /translation="MRAAALDFGTVRIGLAVADELGLLAHPRPHLNGADLEGAFKQLE
                     ALVEAEGIELFVVGLPRSLDGKEGPAAKRARVFAKRLKGRTKARVVLVDEWLSTKEAT
                     QRLREQGLDSRAQRSRVDSAAAAVLLQSYLDRGQASGP"
     gene            10817..>11841
                     /gene="hisS"
                     /locus_tag="VFQ61_13435"
     CDS             10817..>11841
                     /gene="hisS"
                     /locus_tag="VFQ61_13435"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET9955508.1"
                     /translation="MQFRAVKGMNDIVPGDIERWQRLEAAFRKTVELHGYREVRTPIL
                     ESLELFVRSTGETTEIVEKQMFELERSGERLALRPEGTPGAARAYIGGNWHGREPVTR
                     WYYVGPMFRAEQPQRGRYRQFHQAGCELYGDTGPLCDAEMIDMLVGLFQRLGIQNLEV
                     KINSLGGAGTRARYREQLVQFLTPKQANLSEHAQRRLLDNPLRILDSKDPRDREAVQG
                     APTILDALEPEDRAHFEGLTRALDALGTPYQVDTGLVRGLDYYTRTLFEFVGSGGALG
                     TQNALLGGGRYDGMLRGLGGPDAPAIGFAMGLERVLLAMPEATPEKRNSVFIAPVGAR
                     AAVEGLV"
BASE COUNT         1997 a         3888 c         3822 g         2134 t
ORIGIN      
        1 cgggatcaag cgccgctcac cagggcgtag cgtgacggag gtcaacagag cggcttcgag
       61 atccagccct gccgcgcccg agctttggta gctcggcata tccacggcta cgctgcccgt
      121 gcgttgaacg cgaatctcga cgggcgatgc ggacgtgggt gcggctgaca cggataccga
      181 ggaggagttg gctgtcgatg tcatggcggc ccaagatata gcccggatcg cgcgctgcgg
      241 ttagattccg gcgcaattgg cgcccattgc gcgtaagtgc gcgatccgcc tgcgatcacg
      301 cacgatccac cctggtcgag gcgtcggcgc ggctaggctc cacgcgatgg cgcgcaaaga
      361 tctcgtccgc catcgcggac agcttcttcg cctcacgcac gatgtctgga ttgtggcggt
      421 agtgaggcga accctcgacg tagctcgaga aggcgcggta gctcttggct acctcgaact
      481 cgttgccgat ttcctggcag attgcgatcg agcgtaggaa atagtcgacg gctttgcctt
      541 cgtgtccttc accccacgcg cccgcggccg tcacctcacc gagcgtgcgg agcgccatcg
      601 ccaagtgggg cttgctgcga acctggccaa acaagtccac ggcccgtttg atcgcttcac
      661 gcgcgcgctt cagatcaccg cgaacgagat aagctttgga cagcatgcgc ttggcctccg
      721 ccaaatgcag cttgtcccca agctcgtcgc acagctcttc cgcttgcacc agcacgcgga
      781 tcgcgctgtc gctgtcggtg agacggaggt gcgccgcgcc catgttcgtg agcacgatcg
      841 cgatgcggtt gtgttcgctg acttcgctcg cgacctcgcg ggcctcttgg aaaagggcca
      901 gcgctcgctc gggctcgttc tggtcgaatg ccagctgacc gaggtcgttc aggctctcca
      961 cgatccccag cgggtcgttc agctcgcgcc gaatcttgag ggccgcctcg agcgcctcgt
     1021 gggcctccgt gacgcgcccg aagtcccgcc acacgagccc gatgttgttg aggctcaacg
     1081 cgatcgatcg cccatcgccc aaggtcttgc gcatctctag cgcatggcgc atttcgtcga
     1141 gcgccgcggc gtactctccg cgcatccaca gcagtcgccc gatatcgtca tggcaactcg
     1201 cgacgccgcg ctgatcacca ccactctcga acaaagagag cgccgcctcg aaatgctgct
     1261 gcgcctgccc gagtgatccc gtatctcgat acaggcgccc gatgcggttg tgcgcggcgc
     1321 cgcccttggt gggcagcccc aggcgatagg caattgcgcg catctcgcgg aaagccgcga
     1381 acgcctcgtc ggtgcgcccg agcaatacca acacgtcccc gtggttgtgg agcgcgtcga
     1441 tgcggatgcg ggcgtcgtct tcgccgagaa gctcgagtcc cttacggtag tattcgtcgg
     1501 cgcgcttggc cgcgtagtgc tcgcgcgcct tgtgtccgga ctcgatataa gtgaacgcgg
     1561 cccgggtgag cgaacccgcg ttctcgagat gacgcgccag catggccgtg tactcttcct
     1621 ggctgcggac cgtctctttc tgtgccagcc agtccgcgat ggtctggtgg tagcgtcgag
     1681 tcgtggcggc gggcgtgagc cggccgaggc tctcgcgctc caggttgtgc ttgaaaacga
     1741 actcgctctc gcccgcgaac gcgctatccg gaagccggag cacgtagtca cgctgctcga
     1801 gatcggtgag cgttcgcttg acccgctcgc gatcttcgag gctttcgcgg gtccaaaact
     1861 ccggcggatc gcgatcgatg cgcgtcaacg ccacgagggc gttcaaccag aatacgctgc
     1921 ccaacgcggc cgcgtactcc aacagccggc gctcgtgggg cgcgagcgcg aggatgcgga
     1981 gctcgaccgc gtcggctacg gtcagcggaa gccgcgcgct cgctagccga tccagatcga
     2041 cccggaagcc ctcgagctcg ttgccgacca gcacctcggc gtcccgaaag acccgcacca
     2101 tgtgcacgag atggccgggt gtgcctcccg ccatgctgat ggccgcctcg agcagccgcg
     2161 gtggagggcc gccatgacat gcggcaagca ggcgctgcaa aatcgtgagc gagtcgtcct
     2221 ccgacagggg cccgagttcg agcagtgcgt gccgcgaacc gccaagcgtg gaccactccg
     2281 ggtgccggct tagaaactcg ggcctggccg tcccgagcac caagattttg gcgcgcaggt
     2341 tctcgagtaa atacgcgagc agctcgagcg agtcctcgtc cgtcgcatgc aagtcgtaaa
     2401 gcacgaggca gacgcagctc ttggccgcat ctgcctcgat gaaggagcgc accacggccc
     2461 tccgcaacaa gcgggcttgc gaagggtcat cggcaaccgc acgggtcagt gggctttccg
     2521 cgaaggcgag ccccatcagt tcaccgagga aatagcagac gtcccctacc cggcgatctc
     2581 cgagcaccgc gctcacctgc gcgtgcaggc gagtccgcgc gacgtcggat tccgtaccct
     2641 cggggatctc gaagcgcgag cgcagcaagc tggcgaacat cccgtacgct gcggactgag
     2701 cggcgggacg tccctcacac acccgcgccg gcaacggccc tgccccgagc tcctgggtca
     2761 gttcggaaat caggcgtcgc ttaccgatgc cttgcgggcc gaccagcgtc acgatgcgcg
     2821 atcccacccc ttcggccaca tcgtggtacg cgcgccgcaa gagggagagc tcttcgtcgc
     2881 gtcccaccaa cggttccgag gcagatgccg tacggccaag catgtgtggg cggcatcatg
     2941 tctcggttgc ggctccagtc tcaagcgcag cggcaaaatg ccgtcgcgct gcgttcgctc
     3001 ggccggcgtt agcggtcagg tgttgccgct cggccggcgt tcggatcgaa gcactagagg
     3061 tctggactcg cgcccgacgg ggctcaaacc gggcaatttg catttaaatc gcccgtccag
     3121 ctctccgcca agtgccgcct tgcggccgtc cgcggcgagc ggctcgcagc ccgattcatg
     3181 cccgctcggc gaccggccgt ggcgcgctgc ggcgcgcctt tgctgatggg gctgttgcta
     3241 agtgctgcga tgtattagag cctcgagtcg gcttttgcta ggctgcttcc tctcgtgacc
     3301 ctcccggaac gtactccagc ccagtccacc agcgccgaga aacgctcggt gggcaaaggg
     3361 cttttcagca agtgtgctgg ctgcggcgtg gttttcaccg ccgaagagtt cagcagtcga
     3421 tggaatgtgt gcagcagttg cggccgccat cacccgatgg gccgggcgca atggcgcgag
     3481 ctcttgctcg acgatgcggc actcgacgtg tgggacgatc acatcgctcc catcgatccg
     3541 ctgaagttcg aagatggccg cccttatcgt gagcgcgtgg agcgtgccaa acgcgccacc
     3601 tctacgagcg aagccgtaga aatcggccta gctcgtatcg aagggcagcg catcgggtac
     3661 ggctgcttcg tattcgggtt catgggcggc agcatgggct cggtggtggg cgaacgcatc
     3721 acgcgcctgt tcgagcgtgc agcctccgag cgcttgcccg tactcctgct gcacgcctcg
     3781 ggcggagcac gaatgcaaga aggcatcttg agcttgatgc agatggcgaa gaccgtctcc
     3841 gcgttgcgtc gctttcgaga gtgtcgaaag ccctacatca gcttgatgct gaacccgact
     3901 accgggggtg tggccgccag cacggcgttc ctcggggaca ttaatctcgt ggagcccaac
     3961 gcactcgtcg gtttcgcggg gccgcgcgtg attcaaaaca cgctgcgcac gcagctgccc
     4021 gaaggcttcc agagctccga ttttctactc tctcacggca tggtggacgc aatcgtgccg
     4081 cgcgccgagc tgcgcggtaa gttggttcag ctgctgcgcc atctcggggg tgacgtcgcg
     4141 tgacactcga tctcaagaag actctcgagc gtctgtatgc attggggtca cgtggtgcga
     4201 agctcgggat cgagcggatg caggccgcgt gcgctcgctt tggaaacccg gagcgtgcgt
     4261 tcaccagcgt gcacgtcgcg ggaacgaacg gcaaaggcac cgtggtggcg ttcgtgtccg
     4321 ccatggggcg ggccgcgggg ctgcgcgtcg ggacctatac gtcgccgcat ctccatcgct
     4381 tcgccgaacg catcacgatc gatggcaccc cgatcgatga tgcggtgctg gttcctctgc
     4441 tcaaccgcgt gatggacgcg gcgccggatc tcaccttctt cgaggtggcg acgttggccg
     4501 ccttcatcgc cttccgtgat gcgggtgtac agctcgcagt gctcgaagtg ggcttgggag
     4561 gtcggctcga cgcgaccaac gtgattcctg cgccgcgcgt ggcagcgatc acgcgcgtcg
     4621 cgttcgatca catggccgag ctcggagaca cgctcaccaa gatcgcgacc gagaaggccg
     4681 ggatcatcaa gccaggttcg gcggtggtgc tgggcaagct tcaccccgac ggacgcgccg
     4741 tgatggaacg gcgtgcggcc gaggtgggcg cgcgcctggt tccactcggc agccccgagc
     4801 cgataccagg ggctcccctc gcctatccgc gggtcgcgct actcggttcg aacttggccg
     4861 tcgcggtcac ggtcgggcga gagctcggct gggcccccga ggttctggct caaggggtcg
     4921 aaaacacggt ttggccaggg cgcaacgagc tgctccaccg caatgggatg gatctgactc
     4981 tgctcgattg cgctcacaac cctgacggcg cggtggcgct ttcgcacgcg ctcgatccga
     5041 ccgtgctcgc cgacatcgaa tcccggcgcg aaatcgcgct ggtgtttggc gcgctttcca
     5101 ccaaaaactg gcgcgcgatg ctgcgtcggc tcgagcaggt tgccgggcag cgggtatacg
     5161 tcgcgccccc ggtagccaag gcggctgacc cgaggcaaat gctggaggtc gcgagcggcg
     5221 aagtggcgct cagcgtcgaa gaggcgctga cccgggcacg cgagctcgtg ggtccgaagg
     5281 gtgtggtggt cgtcgcaggc tcgatcttct tggtcggggc ggcgcgcgct ctgctgctca
     5341 acctaccgac ggatcctccg atcgcgctat gagccgcagc gctcatcgcg tccgcgcgat
     5401 ggactgatat gacacaacga aagctgtttg gaaccgatgg gattcgcggt cgcgccaacg
     5461 tcgcgccgat gactccggaa ttggcgctcc gcctcgggcg cgcgctggcg tacgtggctg
     5521 gccgcggcaa gtcccgggcg ccccgtgtgt tgatcggcaa ggatacgcgc ctctccggct
     5581 atatgctgga gaccgcgttg gcggctggca tttgcgcgat gggtgggcgc gtgatgttgt
     5641 gcgggccgat ccctacgccg gcggtcgcga acctcgctca gagtatgcgc gccgacgccg
     5701 ggctcgtgat cagcgcgagc cacaatccgt acgcggacaa cggcatcaag atctttggtc
     5761 cggatggctt caagctgcca gactccgaag aggccgaaat cgaggcgctg ctagaagatc
     5821 cgcggctcga caaacttggc cgcacaggct ccaaggttgg gcgcgccgaa cgcatcgacg
     5881 acgcgatggg ccgctacatc gtgtacacca agaatacgtt cccacgggag ctttccctgc
     5941 acggcattcg tgtggtggtc gatgcggcgc atggcgctgc ctacaaggtg gcgccgcacg
     6001 tgttcgagga gctcggcgcg gaagtcgtct cgctcggggt gcgaccgaat ggcaccaaca
     6061 tcaacaagaa ctgcggcgcg ctccaccccg agcatgttgc gcacgaggtg aagcggaagc
     6121 acgctcagct cggcatcgcg ctcgatggtg acgccgatcg cgtgatcttc gtcgacgaga
     6181 aaggcaacat cgccgacggc gactcgatca tggccctgtg cgggctacgc atgctgacgg
     6241 agaagcgcct cagcaaacgt acgctcgtca cgaccgtgat gagcaacatg gggctcgaac
     6301 acgcgctcgc acgagcgggc ggcaagttgg ttcgcacggc ggtgggagat cgctacgtcg
     6361 tcgaggagat gcgccgccac ggctacaact tcggcggcga acaatcgggg cacctagtgt
     6421 tcgccgagca cgccaccacc ggtgacggca tcgtcgcggc gctgcaggtg ctcgcgataa
     6481 tcctgcgcga gggcaagccg ctgagcgagc tgtgtcaggt aatggagcga gtgccgcagg
     6541 tgctcgagaa cgtgagcttg cctgcgcgtc gtccgattca ggagatgcgc gagctgaccc
     6601 gtcgcatcga acaggcggag cgcaagctcg gtaagaacgg ccgcgtgctc gtgcgttgga
     6661 gtgggaccga acccaagcta cgtctgatgc tcgagggacc cgaaccctcg atcctgcgca
     6721 gcttggtcgc cgaaatgaag aacgccgcca agaaggactt ggcggcgagc gcctgattcg
     6781 tcgtcggcag cggaagcggc cgacgattgg caggacgggg cgggctctcg cgtcgagccc
     6841 gagccggcga ctccggacga ggaaggcacc tacagggaag tgccttcccg tcgcgagtcg
     6901 gtcagctcag gtgctgccct tggccgaagc gctggcagac gcgctggctt ggacggacac
     6961 cttgatcgac accgaagcct tggcttgagc ttcgatcgaa gctgccaagc aggtgcccac
     7021 cttgagagcg gctgcgccgc caccggtgac cgcacccttc acgccctcga gcgaagcctg
     7081 cacgttaccc gcgaggctgc cgagtttgtc cttcatgccg acgctcacct tgagcagcac
     7141 cggcaggttc ttctcgagcg cggccttcag cttggccgcc gcctgcgcgt ccgcagaacc
     7201 ttcggccttc accgcgacgt gacccggcgt gcactccagc ttcgccttga cctcggcgtc
     7261 acagttggcc ttgcactccg cgctcatctc tggcggcttc acctcgccgc tgcacttcgg
     7321 ctccttgaac tccacgctgc agccgcccga gcaggtgccg ttgcactcgg ccttggcttt
     7381 catggtgcat ccagcactgc agtcgccctt gcactcgcca ccgcaggtgc cgctggcctt
     7441 gccggcacag gtgccgtcgc aggtcccggc gcacttgccc ttggagtcct tgccgtcgca
     7501 cttgccgttg caggtgccat cgcacttgcc cgagaagtcg gccttgcact cacccgtgca
     7561 gttcgcggca caggtgcctt cgcacttggc ggcaccttct acggtgcatt cgccttcaca
     7621 cttgccttcg cacttgccgc tgagcttgcc gccctcgcac tgaaccttgg cttcgccggg
     7681 cttgatcttg gcgtcgcact tggcggcaca atccgccatc gccgacatcg aagccgcgca
     7741 ttgcggaggc ttcgcggtga ccatgacttt gccttgcgct ttggctttgg tctcggcgat
     7801 cagcttggaa gcaacattgc atgctgcttc ggcgcccttg cccggaccgg cttcggcggg
     7861 cttgagctgc tcgtccgttg cacccaaatc cttggccaga ttcgagcaag cggtggtgac
     7921 ctctccctcg acatctacgg cgatcttttg caggttggcg cccgcggcga gcgcggcttt
     7981 caccttgcct tcgagctcgc cttcgagccc aaagttggca ttcatgatag cggcggcgtc
     8041 agcgatgttc gcggggcacg cgccgcccac atccggtact cccggcacgc tgggcagatt
     8101 gttggcaccg gggacctttc cacactgcgt gagcagcgcg ggcgctgaca acagcatcac
     8161 ccacgcgatc acgaacccgg ttttcttgcg acctgtcgaa gcggacatcg gtaatcttcc
     8221 tcctagcggc cgctcaggcc gcaacaccgt cggagaagct acacacattc cgggctatcc
     8281 aggaaccaaa aacaggcccg cactgacaga cctgcaattc cggccggtcg aacatgttta
     8341 gggtcggcca ttcagcccgc gagcaactcc gactgtcgaa ctcggacact gcctatgtgg
     8401 gtatgggcat ttcgagcttg ttttatgcgg tttccagctg tcgagtcggc ttattcattc
     8461 gcttcgataa gccgccccct gcggaaccta tcgttcaatt gccagttgtc agcgctcgct
     8521 gattggcttt ttgcgggagc gactcttgag ctccgatggc tcggtcttgg ccgcgcgatt
     8581 gctcacttcg cggtcggcac tcgacggttc gcgctcgccc gaatggccat gtttggcggc
     8641 ctcactcgca tcggccctgc tcacggactc gatattgcct gcgaatggca gctccatttg
     8701 cgagtcattg ccgggtttct tgcgcacgct atgagttacc agttccagta actcttgctc
     8761 ttgtcgaata tccgcttcga tcacctccgg attgtccaag gcgaaggcga gcgcatcgaa
     8821 gactttggcc ggaccgagct gcgggaatcg tttcaggatt ttgtccacgc tcgtgccccc
     8881 tcgataaaaa gcccataacc gccgcacggg cacgcgcgag ccgaccacgt ggggacttcc
     8941 accaagcaca ttggtgtcga cgcgaacgtg cgggtggggc accaaaacaa tcggcagttt
     9001 catggtgttg aattcagtgt gggggattcg atggcgcgac gatgatcttc gaagctgcga
     9061 ctgaagcgat ggcgaccgcg cccgtcggcg acgaagtaca gatagtcggt gcgagcagga
     9121 ttgagcaccg ccaaaatcgc gtcctctccc gggttggcga tcggcccggg cggcaaacca
     9181 gggtggcggt acgtgttgta gggattgctc gcgtcccgca gcaactcggg cgtcaccttc
     9241 ccggtgaacg cctggcagct ctgagcggga acgcgcgcca ccccgcagcc gtaagcggcc
     9301 gtgggatccg actgcagcat ccgtgcaggt cggaagctgg aatcatccag acgattgtag
     9361 aaaacgctcg caatcagcgg gcgctcctca cccaccgccg cctcgcgctc gacgatggag
     9421 gcgagggtta ggacctcgcg ttcagtccag tttctttcgc tggccacgcg ttcgatgccg
     9481 cgtccggatc gctcggataa ccgcgccagc cttcggcgcg tctccgccac catcaagcgc
     9541 acggtgaagg ccgcctcgga gttgacgaag agctcgtaag tggcgggaaa caaatagcct
     9601 tcggcgctcg gcccggagat cgtgagctcg gccatcagcg cagagtccgt cgcggcctcc
     9661 aagaactcgt gggcagtaca gacgccgcgc gcctgtagac gctcggcgat ctgcagctgc
     9721 gtgtagccct ctgggatcgc caccttgact cgaggtcgcg aaggcagccg cccgatccgc
     9781 tgggtcagct cccgcggtga cagtccggtt tcgagcagat gcgctccttt cgggactttg
     9841 agaccgccgg caaaccactc gagatagagg gaatacagaa aggcgttcgt gaccagcccc
     9901 cgatctccga gccgcttgcc cgtctcggcc aagtcttcgg tgcccgtgaa ggacatcgtg
     9961 accggcgctc gagcaccggg cgcctcgcgg gtggcccaga ccagaattgc ccctgcacag
    10021 gagagcgccg cgacgaccaa acacagcgct ccaccccaga acaggcgtcg cgccttggtc
    10081 cagcgacgtc gtggtcgggg ccccgggggt gcgagcgagc gtgagctcgg cggccgggaa
    10141 cgacgcgagc ggccgccccg agccgcttca ccgctggagc ctcctcgagg ggggcgggca
    10201 ttagaaccac cactcgagcc cggcttgccc gacccggggc gctcggatcc tgccgtcatg
    10261 gcccgctagc ctgcccgcga tcgaggtagc tctgcagcag aaccgccgcc gcggcgctgt
    10321 ctacccggga gcgttgggca cggctgtcca atccctgctc tcgcagccgt tgagttgctt
    10381 ccttggtcga gagccactcg tcgacgagca ccacgcgcgc tttcgttcgc cccttcaaac
    10441 gcttcgcgaa gacgcgggca cgcttggccg ccggcccctc cttcccgtcc aacgatcgcg
    10501 gcagacccac cacgaacagc tcgatgccct cggcttccac cagggcctcg agttgcttga
    10561 aggcgccctc cagatcggcc ccgttcaggt gagggcgcgg gtgggccagg aggccgagct
    10621 catcggcgac cgccagaccg attctaacgg ttccgaagtc cagcgcggca gcacgcatgc
    10681 aatgggctgc ctcttagcac gcctcgggcg tgcgaataat cccgccgttg cgcatcccga
    10741 gcttgacccg acccctgcca ccgagcataa gcggcgggcg cgcggcccaa cgctcgcgca
    10801 acgccccgga aaaatcatgc agtttcgcgc cgtcaagggg atgaacgaca tagtccccgg
    10861 agacatcgag cgctggcagc gcctcgaagc agcttttcgc aagaccgtgg agctccacgg
    10921 ttaccgcgaa gtgcgcaccc cgattttgga gtccctcgaa ctcttcgtgc gatctaccgg
    10981 cgagaccacc gagatcgtcg agaagcaaat gttcgagctc gaacgcagcg gcgagcgatt
    11041 ggcgttgcga ccggagggca ctcccggcgc agctcgcgct tacatcggcg gcaactggca
    11101 cggtcgcgag ccagtgacgc gctggtacta cgtgggcccg atgtttcgcg ccgagcagcc
    11161 gcaacgcggc cgctatcgtc agtttcacca agctggttgc gagctgtatg gtgacacggg
    11221 gccgctgtgt gacgccgaaa tgattgacat gctggtgggg ctgttccagc gcctcggcat
    11281 tcaaaacctc gaggtgaaga tcaactcgct cggcggtgcg ggtacccgcg cgcgctatcg
    11341 cgaacagctg gttcagttcc tgacccctaa gcaggccaac ttgagcgagc acgcgcagcg
    11401 acgcttactc gacaacccgc tccgcatcct cgactcgaag gatccccgtg atcgcgaggc
    11461 ggtgcagggc gcgcccacca tcttggatgc gctcgagccg gaagatcgcg cccacttcga
    11521 gggcctcacc cgagcgctgg acgcgctggg cacgccctac caggtggaca cgggcttggt
    11581 gcgcggcctc gattactaca cgcgaacgtt gttcgagttc gttggcagcg gaggggccct
    11641 cggcacgcaa aacgcgctgc tcggcggtgg ccgctatgac ggtatgctgc gcgggctcgg
    11701 cggtcccgat gcaccggcga ttggttttgc catggggctc gagcgcgttc tcttggccat
    11761 gccagaggcc acgccggaaa agcggaacag cgtgttcatt gctccagtag gcgcacgggc
    11821 ggcggtggaa ggcttggtat t
//
LOCUS       DASTKU010000389         3792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_73306,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000389 DASTKU010000000
VERSION     DASTKU010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73306"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFQ61_13440"
     CDS             complement(<1..644)
                     /locus_tag="VFQ61_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955509.1"
                     /translation="MAVRSAALHMAQGLRVLFISPLFSPFQIEVGEALNRCPGIDYRA
                     VFPMRLREGRRQPHWARYDYDASRYEVMPEAQEPEAWLVQRIRELRPDVLISGQSMGP
                     LYSAVLRTWPEQEYRLGFWMEPPDPFKPAARRVATKELTRLRLSLADFVFGIGLRAEQ
                     FYRSCAPQADVTLVPYGEDLSACFAASERDKPSDKLSFLFSGQLIPRHNIPWIL"
     gene            929..1708
                     /gene="rfbF"
                     /locus_tag="VFQ61_13445"
     CDS             929..1708
                     /gene="rfbF"
                     /locus_tag="VFQ61_13445"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HET9955510.1"
                     /translation="MKAVILAGGLGTRLSEETVLKPKPMVEIGGKPLLWHIMKIYSQH
                     GIVDFLICAGYRGYVIKEYFANYFLHMSDVTFDLRNNTLEVHRNQAEPWRVTIVDTGS
                     NTMTGGRIKRVAPYVGNETFLLTYGDGVADLDVQASIAFHRAQGRAATLTAAQPPGRF
                     GQLGISPNHEVHAFEEKPRGEGGWINGGFFVLEPSIFDFISGDETVWEREPLQALART
                     GQLSAYKHDGFWACVDTLRDRNYLEALWSADKAPWKTWRDS"
     gene            1708..2853
                     /gene="rfbG"
                     /locus_tag="VFQ61_13450"
     CDS             1708..2853
                     /gene="rfbG"
                     /locus_tag="VFQ61_13450"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HET9955511.1"
                     /translation="MSSAAQPRTEPGTSRVELGGSVNGDFWNRRRVLVTGHTGFKGAW
                     LTYWLARLGAEVAGYALEPATEPSLFRALGLDELCQHEIGDVRDAGHLGRVLRAFQPE
                     VVLHLAAQAIVLTSYEEPVETFDSNVMGTARLLNELRTCPSVRACVVVTSDKCYENRE
                     WIWGYRELDPMGGNDPYSASKGAAELVTHSFRQSFFSAGRYSEHRLCIASARSGNVIG
                     GGDFSAFRLVPDLARAFAAGLPAEIRNPNSVRPWQHVIDPLRGYLLLAQAGCEHGPRY
                     ARGWNFGPAESDTAPVAEVARRFAEAYGDGARCVIAPVSGAPHEAGLLRLDCSLARAE
                     LGFTPNVGLQNAVTATADWYREFFSGAPRNALRELTWGQLSRFGGVV"
     gene            2855..3775
                     /locus_tag="VFQ61_13455"
     CDS             2855..3775
                     /locus_tag="VFQ61_13455"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9955512.1"
                     /translation="MRVLVTGATGFLGAAVVRHLRHEGHETWAAERAPTSPSRPGMER
                     IQLDLSDRDRIEAVVKQVAPEAIVHCAALGTSAPERDFVALADVTITATLALHAAARR
                     AGVARFIHIGTAFEYGPHDAPVDESTPCNPLGAYAVAKAAASSLLMSAATMHGLPPVV
                     LRLFNLFGPGDKPGRLGSDLVACARSGNPLALSAGAQRRNFMFVEDAARVVSALLGLA
                     PEQNPAGRVLNVGFDDSCTVREFAERFARELGISALLRFGERTADRDKPHHLYSECRA
                     FAEFCRASGLTHLLTKTPLDRAAAQCLGSS"
BASE COUNT          716 a         1164 c         1239 g          673 t
ORIGIN      
        1 tgcaaaatcc acgggatgtt gtggcgggga atcagctgac cagaaaacaa gaacgagagt
       61 ttgtccgaag gcttgtcgcg ctcgctggca gcgaaacacg cgctcagatc ttctccgtag
      121 ggcaccaacg tcacatcggc ctggggcgcg cagcttcggt aaaactgctc ggcgcgcaga
      181 ccaatgccga atacgaagtc tgccagcgac aggcgtaatc gagtcagctc tttggtggcg
      241 actcggcgcg cggctggctt gaacggatcg gggggttcca tccagaagcc cagccggtac
      301 tcctgctcgg gccacgtccg cagtaccgct gaatagagcg gccccatgct ttgaccggaa
      361 ataagcacgt ctggccgcag ctcccggatg cgttggacga gccaagcctc aggctcttgt
      421 gcctccggca tcacttcgta gcggctcgca tcgtagtcgt aacgagccca gtgcggctga
      481 cgacgcccct cgcgcaagcg catggggaaa acggcgcggt agtcgatccc ggggcaacga
      541 ttcagagctt ccccgacttc gatttggaac ggtgaaaaca gcggagaaat gaagaggacg
      601 cgcagacctt gggccatgtg gagagccgcc gagcgcacgg ccatgtggcg cactactcga
      661 gcgatggggg actctacacg gccgggccgc gcggagccaa gctcatcagc cgcgccctca
      721 cggctatcct atggtacacg gcaaatccaa tgactttcct gcagtccgga gccgaaccga
      781 cttcgcgggt atgcgcgtat ccgcccgcag cggggcggag ggtccccgcg gagcggttct
      841 ccaccactga ttggtcgaag ccgagaacga gtgccgcgtg gcttaaggcc gcctccgtga
      901 gctcggcctc gatcggaaga ggcgagccat gaaggccgtg atcttggcgg gcgggctcgg
      961 aactcggctc agcgaggaga cggtgctcaa gccgaaaccc atggtagaaa tcggcggcaa
     1021 acccctgctc tggcacatca tgaagatcta tagccagcac gggatagtcg acttcctgat
     1081 ctgcgctggg taccgcggct acgtgatcaa ggagtacttc gcgaactact tcttgcacat
     1141 gtcggacgtg accttcgatc tgcgcaacaa cacgctcgaa gttcaccgaa atcaggcgga
     1201 gccgtggcga gtcacgatcg tcgacacggg ctcgaacacc atgacggggg ggcgcatcaa
     1261 gcgcgtcgcc ccctacgtcg gaaacgagac gttcctactc acttatgggg atggcgtcgc
     1321 cgacctcgac gtccaggcca gcattgcctt tcaccgcgcg cagggccgcg ccgcgacatt
     1381 gacggccgcg cagccgccag ggcgatttgg ccaactcggc atatccccca accacgaggt
     1441 ccacgccttc gaagagaagc cacggggcga agggggctgg atcaacggcg gatttttcgt
     1501 gctcgaaccg agcatattcg acttcatctc tggagacgag accgtttggg aaagagagcc
     1561 attgcaggct ttggcgcgca ctgggcaact ctcggcttac aaacacgacg gcttttgggc
     1621 atgcgtcgat acgctacgcg atcgcaacta cctcgaagcg ctgtggtcag ccgacaaagc
     1681 gccttggaag acctggaggg atagctgatg tcgagtgcag cgcagccgcg caccgagccg
     1741 ggaacttcga gagtagaact cggcggaagc gtgaacggtg atttttggaa ccgtcgtcgc
     1801 gtgctcgtca ccggacacac cggtttcaag ggcgcttggt tgacgtattg gttggcgcgg
     1861 ctcggagccg aagtcgccgg gtacgcgctc gaacccgcaa cggagccgag cctgttccgc
     1921 gcgttgggtc tcgacgagct gtgtcagcac gagatcggcg atgtccggga tgcgggccat
     1981 ttgggccgcg tgctgcgcgc cttccagccc gaagtggtgc tgcacttggc ggctcaagcc
     2041 attgtgctca cttcttacga ggagccggtc gagaccttcg acagcaacgt gatgggtacg
     2101 gcgcgactgt tgaacgagct ccgcacgtgc ccgagtgtgc gcgcctgcgt ggtggtgacg
     2161 agtgacaagt gttacgagaa tcgagagtgg atctggggct atcgcgagtt ggacccgatg
     2221 ggcggcaatg atccgtacag cgcgagcaag ggcgcggccg aactggttac acactccttc
     2281 aggcaatctt tcttctccgc cggtcgctac tcggagcacc ggctttgcat cgccagcgca
     2341 cgttccggga atgtgatcgg aggcggggat ttcagcgcgt tccggctcgt tccagacctg
     2401 gcacgagcgt tcgcggcagg tctgcccgcc gagatccgca atccgaacag cgtgcggccc
     2461 tggcagcacg tgatcgaccc gctacgcggc tatttactgc tcgcgcaagc gggttgcgag
     2521 cacggtcccc gttacgcgag gggatggaat ttcgggcccg ccgagagtga cacggccccc
     2581 gtcgcggagg tagcgcgacg cttcgcggaa gcctatggcg acggcgcacg ctgcgtgatc
     2641 gcgcccgtca gcggtgcgcc gcacgaggcg ggcctcttga gactggattg cagtttggcc
     2701 cgtgccgagc tgggcttcac gcccaacgtc ggcctgcaaa acgcggtgac agcaaccgcc
     2761 gactggtatc gcgagttctt tagtggagcg ccccgcaatg cgctgcgcga gctgacctgg
     2821 ggccagttat cgcgttttgg cggggtggtc tgaaatgcgc gtgctggtca ccggggcaac
     2881 cggctttttg ggggcagcag tagtgcggca cctgcgccac gaggggcacg aaacctgggc
     2941 ggcggagcgc gcgccgacct caccttcgag accgggcatg gaacggattc agctcgattt
     3001 gagcgatcga gaccgcatcg aagcagtcgt aaagcaggtc gcgcccgagg cgatcgttca
     3061 ctgtgccgcg ctcggcacct ccgcgcccga acgagacttc gttgcacttg cggacgtgac
     3121 gatcacagcc acgctagcgc tgcacgccgc cgcacgacgc gcgggcgtcg cgcgcttcat
     3181 ccacatcggc accgccttcg agtatgggcc acacgacgcc cccgtcgacg agagcacccc
     3241 ctgcaatccg ctcggagcct atgcagtggc caaagccgcc gccagcagtt tactgatgtc
     3301 ggccgcgacg atgcacggct tgccacccgt cgtactcagg ttgttcaatc tgtttggtcc
     3361 tggggacaaa ccaggtcgct tgggcagcga cttggtggcg tgcgcacgga gtggaaaccc
     3421 gctggcattg agcgccggcg cccaacgccg gaattttatg tttgtagagg acgctgcccg
     3481 ggtggtttcc gccctgctcg gtctagcgcc agaacaaaac cccgccgggc gagtgctcaa
     3541 cgtggggttc gacgattcct gtacggtgcg tgaattcgcc gaaaggttcg cgcgggagct
     3601 gggaatttcc gcactgcttc gcttcggaga gcgcactgca gatcgcgaca aaccccatca
     3661 cctgtactcc gagtgccgag ccttcgcaga attctgccgc gcctcggggc taacgcatct
     3721 gctcacgaag actccgttgg atcgggcagc cgcgcagtgt ttgggcagta gttaatcgca
     3781 gtcgggtcat ga
//
LOCUS       DASTKU010000390         5348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1858620, whole genome shotgun sequence.
ACCESSION   DASTKU010000390 DASTKU010000000
VERSION     DASTKU010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858620"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1609)
                     /locus_tag="VFQ61_13460"
     CDS             complement(194..1609)
                     /locus_tag="VFQ61_13460"
                     /inference="COORDINATES: protein motif:HMM:NF015617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-reducing end alpha-L-arabinofuranosidase
                     family hydrolase"
                     /protein_id="HET9955513.1"
                     /translation="MNLLRATFAISVTSFLAGCSSSPSPITNTGGAVGLGGLGSGGVG
                     AGGASTGGTSSTGNGGSQPSSSGGAQASGGQASGGTSSAGAAQGGSQASGGNASGGVP
                     NGGASTGGAGGSVGGAGAGGDTSRGGVPSGGAGNGGGGAGNGGRTNTGGGPAMGGGTS
                     AGGSGGSGGGGASCPLPSTFKWTSGGPAALPASGWDSIKDFTYSPHNGKHIIYMTMHS
                     GSSYGAAMMSFADWPAAANATQNKLSFNAVAPTLFYFSPKQLWVLAYQWGATGFSYRT
                     SSDPTNPSGWSGEKSLYASKTVPEGQSDTGVIDQQVICDAAKCYLYFNGDNGYIYRSS
                     MPIDSFPGEFPKATTVMKDTPQKIFEAVQLYSVKGSNKYLMLVEAQGNGRYFRAWTAT
                     DLGGTWTLLTDTFAAKSNTTFSQNWTNDISHGDLVRSGPEETFPIDPCNLQMIFQGRD
                     PNSNVAYDKLPYRLGLLTAAK"
     gene            complement(2129..2677)
                     /locus_tag="VFQ61_13465"
     CDS             complement(2129..2677)
                     /locus_tag="VFQ61_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955514.1"
                     /translation="MFGITAQGSQPMIRMMFQKWSIALLTVFGGVLSSACVHGASTIK
                     PRSIHASTLVGDWQGINDDEPRIFLMRVSESKKARIAMTVGPSNYVDVYAFESTALVI
                     KQDGLVLSAKGINGMRIELRISGVALPNEGMLTGKAVLWPGDGGKMELPVKFYKRRGG
                     YVQELLRLLQASERELSNDERG"
     gene            complement(2851..3339)
                     /locus_tag="VFQ61_13470"
     CDS             complement(2851..3339)
                     /locus_tag="VFQ61_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955515.1"
                     /translation="MLAEPPLEELAPPVVLLTPPLEELAPPVVLLAPPLRELAPPVAL
                     LAPPIVDPAPPLEFAPPIAELAPPVVLLAPPFAELAPPIVVMVAPPVVLLAPPFVTVA
                     PPLATVAPPVVELAPPVVELAPPVVGPPPDAPPPGGAPVSGLVELQAGSTDQVAQTRA
                     QA"
     gene            3338..4057
                     /locus_tag="VFQ61_13475"
     CDS             3338..4057
                     /locus_tag="VFQ61_13475"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET9955516.1"
                     /translation="MTGGATGANAPYKGVANSPAAQLKELGATWCYNWGTAPKSTDCA
                     DPLFVPMIWGSGDVAAAIKQIGAKGYKTVLGFNEPNKSDQANLTVAQAIALWPALTSD
                     PNIRVSSPAVSDDGRAWLEDFMTQAKAKGLRVDFIAMHWYGWNAGSCKASQLQGAVNW
                     ASKWGLPVWITEFGCMGSSNPDEQTVLSFYKDAVTMLQSHPLVERYAWYPWNTYNKLY
                     DDKGVQTALGAALQAAPQYRK"
     gene            4574..>5348
                     /locus_tag="VFQ61_13480"
     CDS             4574..>5348
                     /locus_tag="VFQ61_13480"
                     /inference="COORDINATES: protein motif:HMM:NF013559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HET9955517.1"
                     /translation="MSSKGVSGSVWVGSLLAAVACFSGCGAPVDEASIEDELGVSESE
                     LYPLASRIWRQTSISVCWEAPAVPDANQTTKRGWVKSQVAATWSAATRVQFTGWGDCS
                     ASPAQLRIAVKDEATAPHTIGLGRNLDGKPSGLTLNFTFNNWQNGANNTLDCRPILES
                     CIRKIAAHEFGHVLGFAHEHNRSDKPASCTDAPSGSLGDTLAGPYDPDSIMNYCNPVW
                     NNNGVLSAGDIKAARQYYGTSTESAAQRDTVNWGNGKIYF"
BASE COUNT         1066 a         1691 c         1611 g          980 t
ORIGIN      
        1 gcgccacggt tcagcccccg tggcgctcgc agctttcaca cctaacgtcg tacgggatca
       61 cccggctcac ctcggagcgg ctcggcgcca cggttcagcc cccgtggcgc tcgcagcttt
      121 cacatctaac gtcgtacggg atcaccccac atctaacgtc gtacgggatc accccccccg
      181 tgcggcggcc gcttcacttc gctgccgtca acaaccccag acggtaaggc aacttatcgt
      241 acgcaacatt cgagttcggg tcccgtccct ggaaaatcat ctgcaggttg cagggatcga
      301 tcgggaacgt ctcctccggg ccgctgcgca ccaagtcgcc gtggctaatg tcattcgtcc
      361 agttttggct gaacgtcgta ttgctcttgg cggcgaaggt atccgtcaga agcgtccacg
      421 ttccgcccag atccgtcgcg gtccaagcgc ggaagtagcg cccgttgcct tgggcctcga
      481 ccagcatcag atacttgttc gagcccttga cggagtagag ttggaccgcc tcgaagatct
      541 tttgaggcgt gtctttcatg accgtagtgg ccttggggaa ctcgccgggg aagctatcga
      601 ttggcatcga cgaacggtag atgtagccgt tgtcaccgtt gaaatacaga tagcacttcg
      661 ccgcgtcgca gatgacctgt tggtcgatta ccccggtatc actctgaccc tcgggcacgg
      721 tcttacttgc gtaaagcgac ttctcgcccg accacccact gggattagtt ggatccgacg
      781 aagtgcggta gctgaaaccg gtggcacccc attggtaggc gagcacccag agctgcttcg
      841 ggctgaagta gaagagcgtc ggcgccacgg cgttgaacga caacttgttt tgcgtcgcgt
      901 tcgccgccgc cggccaatcc gcgaagctca tcatcgctgc gccgtaagac gagccggagt
      961 gcatcgtcat gtagatgatg tgcttgccgt tatggggcga gtaggtgaag tctttgatcg
     1021 agtcccaccc acttgccggc aaagcagcgg gcccacccga ggtccacttg aaggtcgagg
     1081 gcaggggaca actcgcccca ccaccgccag agccaccgct cccgccagcg ctggtgccgc
     1141 cgcccatcgc gggtcctccg ccagtgttcg ttcggccacc gttgccggcc ccaccaccac
     1201 cattacccgc gccgcccgag ggcacgccgc cgcgcgacgt atcgccgccg gctccggcac
     1261 ctccgaccga cccgccagcc cctcccgttg acgcgccgcc gttcgggacg cccccggacg
     1321 cattcccgcc cgaggcctga gagccacctt gcgcagctcc ggcactcgac gtccctccag
     1381 acgcctgccc gcccgacgcc tgcgcgccac cagaagagct gggctgcgaa ccgccgttac
     1441 ctgtgctgga agtgcctccg gtgctcgcac cgccggcgcc cacgccgccc gagcccagac
     1501 cgccgagacc gaccgcacca cccgtgttgg tgatgggtga cggcgagctg ctgcagccgg
     1561 ccaggaacga tgtgacagag attgcgaatg tggcgcggag gagattcatc acttggcttt
     1621 gcctcggctt gcgtcagcga agatgtgcaa ttgacctttg cccgatgcag tgggatcaaa
     1681 ccgtgcaacc cgtcaccaaa aacatttcat ccaggctgag ctcaaatttc cagcctgtgg
     1741 cccggttgag cggcgggcat aaaacccgcg atttgagctc gtcgcgcgtt tcttgagctg
     1801 aaccccatca ctccagcgcc accttcgggt ctttccggtc cgaaattgag attagtgatg
     1861 ctctctaacg agggcacccc gcgtagcacg gagcctgcag cgaacagacc agatcggtcc
     1921 gcgcaggagg gccgatttgt ccctagtttc ctgctcccca gcgatggaac acattggcgc
     1981 gccgtgccgt gctcgctgcc gctgcgcgcg gccgaggccc tcaaggccat gctcgcgcct
     2041 ccggcgctgc gcgtggccga gggtcctgca ccgcagtggg gacgtgttct gcacgcatgc
     2101 agccgctcca cctctcggca ccggcggatt atcccctttc atcattagag agctcacgct
     2161 ccgacgcttg aaggagccgc aggagctcct gaacatagcc gccgcgtctc ttataaaact
     2221 tcactggcag ttccattttt ccgccatctc ccggccaaag cacggctttg ccggtgagca
     2281 tgccctcatt cgggagcgcc acaccggaga tcctgagttc tattcgcatt ccgttgatgc
     2341 ctttggcgga gaggaccaga ccatcttgtt tgatgacgag tgcggttgac tcaaaagcat
     2401 atacatcaac gtaattggac ggtccgaccg tcatcgcgat ccgagccttc ttcgattcag
     2461 agactcgcat caagaaaatc cggggttcat catcgttgat gccctgccag tcaccaacga
     2521 gcgtactcgc atggatagag cgcggcttta tggtcgaggc cccgtgtacg cacgcactcg
     2581 agagtacccc tccaaacacg gtgagaagcg cgatagacca tttctggaac atcattcgaa
     2641 tcatgggctg tgatccctgg gcggtgatcc cgaacaagaa gagaagaata ttatgcgaga
     2701 aagaaaatgc tgaatgtctg ggcctacttg tctcgtgcgg gatctgaagc taggagcacc
     2761 aagcagcgct gttggttccg tggcgttcaa caacctcacc aaaatgcgaa gaaacccagt
     2821 gacatccttc tcagcgatag gttctggccg ctacgcctgg gctctggttt gcgcaacttg
     2881 gtcggtgctg cccgcctgca gctccacgag cccggagacg ggcgcgcctc cgggcggagg
     2941 cgcatccggc ggtggcccca cgacgggcgg agccaactcc acaacgggcg gagccaactc
     3001 cacaacgggt ggagctaccg tcgcaagcgg tggagctacc gtcacgaacg gcggcgcgag
     3061 caacacgacg ggtggagcca ccatcacgac gatgggtgga gccaattccg cgaagggcgg
     3121 cgcgagcaac acgacgggcg gagccaattc cgcaatgggg ggtgcaaact cgaggggcgg
     3181 cgcgggatcc acgatgggag gcgcgagcaa cgcgacgggt ggagccaact ccctgagggg
     3241 tggcgcgagc aatacgacag gtggagccaa ctcctcgagc ggtggcgtga gcaacacaac
     3301 gggtggagcc aactcctcga gcggtggctc ggccaacatg acgggcggcg cgaccggcgc
     3361 gaatgctccg tataagggcg tcgccaactc ccccgctgct caactgaaag agctaggcgc
     3421 aacctggtgt tacaactggg gcactgcgcc gaaatccacg gattgcgccg atccgctatt
     3481 cgtccccatg atttggggct ccggcgacgt cgctgcggcc atcaaacaaa tcggggccaa
     3541 aggttacaag acagtgctcg gcttcaacga gccgaacaag tctgaccagg ccaacctaac
     3601 ggtggctcaa gcgatcgcgc tctggcctgc actcaccagc gaccccaaca ttcgagtgag
     3661 cagtcccgcc gtgtcggacg acgggcgtgc gtggctcgaa gacttcatga cccaagccaa
     3721 agccaagggc ttgcgcgtcg atttcatcgc catgcattgg tacggctgga acgcgggctc
     3781 gtgcaaagcc agccagctcc aaggggcggt caattgggcc tcgaaatggg gcctgccggt
     3841 atggattacc gaattcggct gcatgggctc cagcaacccc gacgaacaaa ccgtcctgag
     3901 cttctacaag gacgccgtga ccatgctgca aagtcaccct ctcgtcgaac gctacgcctg
     3961 gtacccctgg aacacgtaca acaaactcta cgacgacaaa ggcgttcaaa ccgcgctcgg
     4021 cgccgcccta caagccgcgc cgcaataccg aaagtagtca ggccgagcga cgcaaacaac
     4081 gcgcttccgg gctgagactc ggagtcacct cgcggggtga gagctgccct gctgagcgct
     4141 ttagcgtggt ctaggtggtc gaaacgctca aggggacggc gtgggggcgt agctgtcgcg
     4201 ggcgtccgag ctagcgtggt tcgggatcac tgggggttga cgcgcttgac ccgagttgct
     4261 cactttgcgc gagttgaccg agttggtcct ccagcaaata ggcaatctcg gtcctctgtt
     4321 ttaacggcag atgcgccaga acgccgcaaa gctgaagaga gaccgcccgc atccgtctgg
     4381 accccggacg gtacgggcgt tgcattgccg ccgcgacgtg cggcagtgtt ccgtgcgcca
     4441 gcccgcgcgg cacctcacct gaacttcacc ggcattcgca tgtccggatg caagaacggg
     4501 ataagtacga tcgagaattc aagtcagaca cttggattcc gaaccaattg atgagactga
     4561 gtaggagaaa aagatgagtt cgaaaggggt cagtgggtcg gtttgggtcg gttcgctcct
     4621 ggcggccgtt gcttgcttta gcggctgcgg cgcgccggtg gacgaggcga gcatcgagga
     4681 cgaacttgga gtctcggagt cggaactcta cccgctcgcg tctcgaattt ggcgacagac
     4741 gagcatctcc gtatgttggg aagctcccgc tgtccctgac gcgaatcaga ccactaaacg
     4801 cggctgggtg aagagtcagg tcgccgcgac ttggtcggcg gcgactcgcg tccagtttac
     4861 cggttggggg gactgctccg cgtcgccggc tcagctgcga atcgccgtta aggacgaagc
     4921 gaccgcccct cacactatag gtctcggacg caatttggat ggtaagccgt caggtctgac
     4981 gttgaacttc acgttcaaca attggcagaa tggcgcgaac aacaccctgg actgccgccc
     5041 gatactcgag tcttgtatca gaaagattgc agcccatgaa ttcggtcacg tgcttggctt
     5101 cgcgcatgag cacaaccgtt ccgacaagcc ggcgtcctgc accgatgcgc cgtcgggatc
     5161 gctaggtgac acgctcgcgg gtccctatga cccggattcg atcatgaact actgcaaccc
     5221 cgtttggaac aataacgggg tcctgagcgc gggtgatatc aaggccgccc gacagtacta
     5281 tggcacctcg accgagtcag ccgcccagcg tgacacggtg aattggggta acgggaagat
     5341 ctactttt
//
LOCUS       DASTKU010000391         5256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_519526, whole genome shotgun sequence.
ACCESSION   DASTKU010000391 DASTKU010000000
VERSION     DASTKU010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519526"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VFQ61_13485"
     CDS             <1..962
                     /locus_tag="VFQ61_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955518.1"
                     /translation="SSSGGSSSGGTTATSSSSGGSSNGGSSGGGSSSGGASSTTGGTQ
                     VSKSLSGDFTVKLTAASDGTPAFTTFSGRANDGPTPPTIVLKVAAESGDCKLSVPDAP
                     FCEKDCGQGVCTAENTCTPYPQGQDLGPLVVKGIGPSPLPLTGTPPNFSYTSGAALPN
                     PPCVEGADFSVEANGLSLTGKCIAPLSLKGADPLPIATGKSLDLTWDPPKTPGQSRVE
                     IYLDIAHHGGKKGQINCDVADTGSFSIPEPLVTQLINLGVAGFPTVQVTRASTAVSSA
                     LPNVRLRMTSMVERSIDTGVASCSEDIPCPNGEMCRLSDYTCR"
     gene            1138..3021
                     /locus_tag="VFQ61_13490"
     CDS             1138..3021
                     /locus_tag="VFQ61_13490"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HET9955519.1"
                     /translation="MTLGRPSRKAARKARAKHAQSKQAASFTPAQRALTWLAPATLGL
                     ACAGAGARVHVNQVGYERRAPKHAVIESEHALAGFRVVSTLDSRVLLDQPMAAQPPAD
                     GWDSGRFVYRADFSQVQEPGEYVIEAGTQRSIRFRIGEQLLFATTAPDVLAYFRHMRA
                     DAPEIWAADAQVPILESNEKRDVRGGWYDASGDVSKYLSHLSYANFLNPQQTPLVVWI
                     LGWTSQQAHGLLQRYGLQQAAAEEAAWGADFLLRMQDESGFFFATVFDGWSGDPSARK
                     LCAYSGIQGSLNTNYRAALREGGGLAIAALARAARGRAGAFPTDAYLQAAVKGFAHLK
                     QHNLEYVDDQTENVIDDYSGLLAATELFVSTSQTDYLEAARERAARLIRRLHPTGYFV
                     ADARERPFYHASDAGLPIVALTEYTKIEPDVTRIDETLSGIARHLNYWVSVTREVPNP
                     FGYARQHVAPGKTSFFIPHDNESGYWWQGENARLASLSAATWLGQRALARRPPSASKA
                     GADAVSPPALESFATDQLAWILGHNPVDVCMLQGFGANNPPPYSEQKRLGGTLRGGIA
                     NGITSRERDEQGFQWSKAVGPGREWERWRWLEQWLPHAAWYFMAITLAAQSEPLVSPG
                     AQP"
     gene            3018..4178
                     /locus_tag="VFQ61_13495"
     CDS             3018..4178
                     /locus_tag="VFQ61_13495"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9955520.1"
                     /translation="MSLLRVAWKNTRRNAGRTATTVLAVAISLVAYVLLQSVSFGYSE
                     QVRQTPNNRVVTRHKLGWAYNMPIHYAAEVRGVKGVVQAMGGRWVGLKVPLHEEFFFE
                     SLAVEPAEYVAMHYELAAPARDKAAFVGNRRGALVSEELAQKFGWKIGDSIVLKSRDY
                     PGEFNLIVSAIFHSTRYGYAQRAIFMHWQYLNESLPLRERERIDVISAQVADPSLGAR
                     LAKDIDIHFDSQDPQSFSMEDQALIASEVGRYGAILSALDTVSVLILGMVLLILGNTL
                     AMGVRERTREYGMLRAVGFAPRHVMALVLGEAALIGLAGGLVGLALAYPIVQRPLSRY
                     LEEAAGFAPLHVPLSVALSCVLAGAALGVLSASTAARGAAKLDVVQALRRVG"
     gene            4183..>5256
                     /locus_tag="VFQ61_13500"
     CDS             4183..>5256
                     /locus_tag="VFQ61_13500"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9955521.1"
                     /translation="MTLPIKYSLRSLWGRRTTTFSTAGGVALVVFVLAACLMLLNGLE
                     RTLGKTSSSSKVLVMQTDAFSEAGSRLRKDSLAHVQAAPGVLRDSDGAPRVTADSIVA
                     VQFTQAQNPSRIVSLQVRGVSPNFQRLRPEVRLVAGRLPRSGTDEAMVGRAIVSRYGG
                     LHLGGSFALKKNRDIRVVGVFEAQGMAFESEIWADIESVQSSLGWQGFVSSVTAELAS
                     PGQFDAFKIALESDKRLGYKVEREAAYYSRMASGLTSVITALGGAVTLIFSIGAMLGA
                     SITMYAAVNQRTQEIGVLRALGFGSAEVLTVFLLETGLVALSGASVGVLLSCFTPLVP
                     FATVNAMTGNEVIVPFVLSPGTLL"
BASE COUNT          939 a         1578 c         1748 g          991 t
ORIGIN      
        1 ccagctcgag tggcggtagc tcgagcggcg gtaccactgc cacctccagc tcgagcggcg
       61 gcagctcgaa tggcggtagc tcaggaggag ggagctccag cggaggggcc agctcgacta
      121 cgggtggaac tcaggtgagc aagagcttgt ccggggactt cacggtaaag ctcactgcag
      181 ccagcgacgg cacgcccgct ttcaccacgt ttagcggtcg cgccaacgac ggaccaacac
      241 cgcctacgat cgtcctgaaa gtcgcggccg aatcgggcga ctgcaagcta tcggtgcccg
      301 acgcgccgtt ctgcgagaag gactgcggtc aaggcgtgtg cacggcggag aacacctgca
      361 cgccttaccc gcaagggcaa gacttggggc cgctcgtggt gaaggggatc ggtccgagcc
      421 ctcttccgct gacgggtacc ccgccgaatt tttcgtatac ctctggcgcc gctctcccca
      481 acccgccctg tgtcgaaggc gctgattttt cggtagaggc gaacggcctg agcctgaccg
      541 gcaagtgtat agcgcccttg tcactcaaag gcgccgatcc gctaccgatt gcaaccggta
      601 agagcttgga cctgacttgg gacccgccca agacgcccgg gcaatcgcgg gtagaaatct
      661 acttggatat cgcgcaccac ggcgggaaga aggggcagat caactgtgac gttgcggaca
      721 ccggctcgtt ctcgattccc gagccgctcg tcacccaact catcaacctc ggagttgccg
      781 gttttccaac cgtgcaagtg acccgcgcgt cgaccgcagt atcgagtgcg ctacccaacg
      841 tgcgtctgcg aatgacttcg atggtggagc gcagcatcga caccggcgtc gcctcgtgct
      901 cggaggacat tccgtgcccc aacggggaga tgtgccggct atcggattac acttgccgct
      961 gagtcgttgg tttccgggtg cggctcgcgc tgcgttttgg catccgcgtg tggccaacgg
     1021 atgagcaaac tgcgtgtacg gtcacgaatg attgaaaaaa gagagaaata gctcactcgc
     1081 gacggattcc acgatcgcgg cgaggtgata ctctgcgcgc ctgtacggag gcgtcgcatg
     1141 acattgggtc ggccaagtcg gaaagcagct cgaaaggctc gagcaaagca cgcgcaatcg
     1201 aagcaggccg catccttcac gccagcccag cgggccctga cctggctggc tccggctaca
     1261 ctcggtctgg cgtgcgcggg cgctggcgca cgagtacacg taaaccaagt gggttacgag
     1321 cgccgcgcgc ccaaacacgc ggtaatcgag tcagagcacg cgctcgcggg ctttcgcgtc
     1381 gtgagcacgc tcgactcgcg agtgctgctc gatcaaccga tggcagccca gcccccggcc
     1441 gacggctggg actcgggtcg tttcgtttac cgggcggact tcagccaagt gcaagagccg
     1501 ggcgaatacg tgatcgaagc gggcacgcag cgatcgattc gttttcgcat tggtgagcag
     1561 ctcttgttcg cgacgaccgc gcccgacgtg ctcgcgtact tccgtcacat gcgcgcggat
     1621 gcgccggaga tttgggccgc cgatgcgcag gtgcccatcc tcgagagcaa cgagaagcga
     1681 gatgttcgag gggggtggta tgacgcttcc ggcgatgtct ccaagtacct gagccacctc
     1741 agttacgcga acttcctcaa cccgcagcaa actccgctcg tagtctggat tctcggctgg
     1801 acgagtcagc aagctcacgg cctgctgcag cgttatggat tgcaacaggc cgcggcggag
     1861 gaagcggctt ggggcgcgga cttcttgctg cgcatgcaag atgaaagcgg cttcttcttc
     1921 gccacggtgt tcgacggctg gagcggcgat ccgagcgccc gcaagctttg tgcatactcg
     1981 ggtattcagg gtagcttgaa caccaattac cgagcggcct tgcgcgaggg aggtgggctc
     2041 gccattgcag cgctcgcccg cgcggcgcgg gggcgagcgg gcgcgtttcc gacggacgcc
     2101 tacctgcaag cggcggtcaa aggtttcgcg cacctgaagc agcacaatct cgagtacgtc
     2161 gatgatcaaa ccgagaacgt gatcgacgac tactccggct tgctcgcggc gacggagctg
     2221 ttcgtgagca cgagccaaac cgactacctg gaagccgcgc gcgagcgagc ggcgagattg
     2281 attcgacgct tacacccaac gggttacttc gtggccgatg cccgggagcg cccgttctat
     2341 cacgcgtccg atgcggggct tccgatcgta gctttgaccg agtacacgaa aatcgagccc
     2401 gacgtgaccc ggatagatga aacgttgtcg gggatcgcac ggcacttgaa ctattgggtc
     2461 tcggtgacgc gcgaggtgcc caacccgttc ggctacgccc ggcagcacgt ggcgccgggc
     2521 aaaacctctt tcttcattcc gcacgacaac gagtccggtt actggtggca aggcgagaac
     2581 gcgcgcctgg cttccttgag cgccgccacc tggttgggac agcgggcgct ggcgcgacgc
     2641 ccgccgagcg cgtctaaagc tggcgcggat gccgtttcac caccggcgct cgagtccttc
     2701 gcgaccgacc aactggcttg gattttgggc cacaatccgg tggacgtttg catgctgcag
     2761 ggcttcggcg cgaacaaccc gccaccgtat tccgagcaga agcgtttggg gggcacgctt
     2821 cgtggcggca tcgccaacgg catcacgagc cgagagcgcg acgagcaagg cttccagtgg
     2881 agtaaggccg tgggcccagg tcgcgaatgg gaacgctggc gttggctcga gcagtggttg
     2941 ccgcacgctg cttggtactt catggcgatt acgcttgcgg cgcagtccga accgctcgtc
     3001 tcgccgggag cgcagccgtg agcctgcttc gtgttgcttg gaagaatacg cggcggaacg
     3061 ccggtcgcac tgcgacgacg gtgcttgccg tagcgatcag cttggtggcg tatgtgctgc
     3121 tccagagcgt cagtttcggc tactcggagc aagtccggca aacgcccaac aatcgagtcg
     3181 taacccgcca caagctcggc tgggcctata acatgccaat ccactacgcc gctgaggttc
     3241 gcggcgtgaa gggcgtggtg caggccatgg gcgggcgctg ggtggggctc aaagtgccac
     3301 tccacgaaga gttcttcttc gaatccctgg cggtcgagcc agccgagtac gtggccatgc
     3361 actacgagct agcggcaccc gcccgggaca aggccgcgtt cgtcggtaac cggcgcgggg
     3421 ctctggtgag tgaagagctg gcccaaaaat tcggctggaa gattggtgac agcatcgtct
     3481 tgaagagccg ggattacccc ggcgagttca atctgatcgt gtcagccatc ttccactcca
     3541 cacgatacgg ctacgcacag cgagccatct tcatgcattg gcaatatctg aacgagagct
     3601 tgccgctgcg tgagcgcgaa cgcatcgacg tgatctcggc gcaggtcgcc gacccgagct
     3661 tgggagcgcg gttggccaag gatatcgata tccatttcga ttcgcaggac ccgcagagct
     3721 tcagcatgga ggatcaggcg ctgattgctt ccgaggtggg ccgttacgga gcgatcctga
     3781 gcgcgctaga tacggtgagc gtgctcatat tgggtatggt gctgttgatc ttgggcaaca
     3841 cgctcgcgat gggcgtccgc gagcgcacgc gcgaatacgg tatgctgcgg gcggtgggct
     3901 tcgctccacg acacgtgatg gcgctggtgc tgggggaggc tgcgctgatc gggttggctg
     3961 gcggtttggt tgggctggcc ctcgcgtatc ccatcgtgca gcgtccgttg agccgctatc
     4021 tcgaggaagc ggccggtttc gctccgttgc acgtgccgct cagcgtggcg ctcagttgtg
     4081 tgttggccgg cgccgccctt ggggtattgt ccgccagcac ggccgcgcgc ggagcggcca
     4141 agctcgacgt ggttcaggcc ctcaggcgag tagggtgaga gcgtgacttt accgatcaag
     4201 tactctctgc gtagcctgtg ggggcgccgt acgaccacct tctcgaccgc gggcggcgta
     4261 gccttggtgg tgttcgtgct cgcagcatgt ttgatgttgc tcaacgggct cgaacgcacg
     4321 ctcggcaaga ccagctcctc gtcgaaggtc ctggtgatgc aaacggacgc gttctcagag
     4381 gccggatctc gcttgcgaaa ggattcgctg gcgcacgtgc aggccgcgcc gggagtgctg
     4441 cgcgactccg acggtgcgcc gcgcgtcacg gcggactcga tcgtagccgt gcagtttacg
     4501 caggcgcaga atccgtcccg catcgtcagc ttgcaggttc ggggcgtgag ccccaatttt
     4561 caacgtttgc gcccggaagt acgtttggtt gcgggccgcc tgccgaggag tggcacggac
     4621 gaagccatgg tgggccgcgc catcgttagc cgctacggag gcctacatct gggaggcagc
     4681 ttcgcgctga agaagaatcg agatatccgc gtcgtcggcg tgttcgaggc gcagggtatg
     4741 gccttcgagt cggagatttg ggcagatatc gagagcgttc agtccagcct gggatggcaa
     4801 ggcttcgttt cgtccgtcac agccgagctc gcgtcaccgg ggcagttcga cgccttcaaa
     4861 atcgcgctcg agagtgacaa gcgcctggga tacaaagtcg agcgtgaggc cgcgtactat
     4921 tcgcgtatgg cgagcgggct gacctcggtg atcacggcgc tagggggcgc cgtgacgctc
     4981 atcttctcga tcggagcgat gctcggggcg tcgatcacca tgtacgcggc ggtgaatcag
     5041 cgcacgcagg agattggcgt gctccgcgca ctgggcttcg gcagtgcaga ggtgctgacc
     5101 gtcttcctgc tcgagaccgg tctagttgcg ctttcggggg cctcggtcgg agtgctgctc
     5161 tcctgcttca ctccattggt gccatttgcc accgtgaacg cgatgaccgg caacgaggtg
     5221 atcgtgccat tcgttctttc acctgggact ttgctg
//
LOCUS       DASTKU010000392         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_295767, whole genome shotgun sequence.
ACCESSION   DASTKU010000392 DASTKU010000000
VERSION     DASTKU010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295767"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VFQ61_13505"
     CDS             <1..795
                     /locus_tag="VFQ61_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955522.1"
                     /translation="KRRNKDAAGGKGPGEDFFDQLVRLHQVYAVGFSDPNWAAQVRGE
                     GASTSTPSHRQRFIDLAREQLSASAIDSMLSAKQFSRVWDELVGILKQTNLVPAAQLK
                     LKFVGAEQERTLAAAVRELLHGSTPYNQRFDRYLSAFAAAFNQPAKWELATAPAAIVS
                     PREHICVELTTFRKQLKLLGTRRAIATQPSSADYTLLLANAQLIAKKLGEQGEAPRDL
                     LDIRDFIVLTLKPPSKAAAAPRPRAKAAARAPSEADDDIADDDQSE"
     gene            1399..3138
                     /locus_tag="VFQ61_13510"
     CDS             1399..3138
                     /locus_tag="VFQ61_13510"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955523.1"
                     /translation="MSKRLFTERSYCASYSRVIFAASFGACTMLGCASNDSPNGGTGG
                     RSASQGGALGSGHGGTNASGASAMSGGASALGGNQGTSSATGGSSGNGQSAGGNANGG
                     TTLGSGGNGAVSSGGRSTAGGSGGGSSAQGGGSNVSTGGNQGGGAAGAGGTTSTGMGG
                     VGGNYVGTKTPGTAQTGDVTVDPAKTHQVVDGFGEADVWQNSSSAAMQTLLWDPVNGI
                     GLTLLRIGIESKSGTSLIMGDAALQDGPACKKFAGDDCKVWAAPWSPPASMKNNDNVN
                     GPGGNNSCSGTNDTLKDGSYEAWASLLAAFPALYKQKSGVDLYAVSAQNEPDFNPNYE
                     ACCYDKNQMVKLINALGPKLAALDPPVKVLAAEPDTWGNLWGGDQYGPAILADAKASS
                     YVGPIATHDYGSSGVTRPSPPSNNTHHVWETECTPSDTGPITIATMIYAAFTSGGANA
                     WHYWWTQQLVPDVKSPPPQVYALGNFSKFVRPGYYRVDVSGAPTPSTSNPLVVAFSNL
                     RDGTVAIVVVNGGSARTLSFFVAGAAWPASVTPYVTTTSSKLAAGTAINVTAGRFSAS
                     LAAQSVTTFVGSP"
BASE COUNT          662 a         1125 c         1066 g          567 t
ORIGIN      
        1 aagcgccgga acaaagacgc agccggtggc aaaggtcccg gcgaagactt cttcgatcag
       61 ctcgttcgct tgcaccaagt ctatgccgtg ggcttctccg acccgaactg ggcggcgcag
      121 gttcgcggcg aaggcgccag cacgagcacg ccgagccacc gtcagcgctt catcgacctc
      181 gcgcgcgaac agctctccgc gagtgccatc gactccatgc tcagcgcgaa gcaattctca
      241 cgggtctggg atgagctcgt cggcatcttg aagcagacga atctggtccc cgccgcccaa
      301 ttgaagttga agttcgtcgg tgcggagcag gagcgaacgc tcgccgccgc cgtgcgcgag
      361 ctactccacg gaagcacccc ctacaaccaa cgcttcgatc gctatctcag cgcgttcgcg
      421 gccgcgttca atcagcccgc caagtgggag ctcgcgactg cacccgccgc catcgtttcg
      481 ccgcgggaac acatctgcgt cgagctgacg accttccgca aacagctcaa gctcctcggc
      541 acccggcgcg ccatcgcaac ccagccgagc agcgccgatt acacgctcct cctcgccaat
      601 gctcagttga tcgccaagaa gctcggcgag cagggtgagg ccccgagaga tctgctggat
      661 atccgcgact tcatcgtcct cacgctgaag cccccgtcaa aggcggccgc cgcaccccga
      721 ccccgcgcaa aggccgctgc ccgcgcgccg agcgaggccg acgacgacat cgccgacgac
      781 gaccagtccg agtaacgccg ttcgggaaag cccgctccgg cgttcgtgac cggagcggtg
      841 tttgcggctg cactgtggtg acggcttgta gggattgccc cgcgcgctcg cacggtgtcg
      901 ttcacgactg gatcgcgctc gagccacccc tcgcaggaag tggcctcacg cgcgcgcctc
      961 gctgcggtct cggcctgctc gcgactgact tgttcattcg cgggccctta cgcggtcagc
     1021 cacgccgttc aggcgggcgc actggcggca cccctgaacc cgcgacctcg gccgtgagcg
     1081 gtccgcagct cgccgtctca ccgctgcagc actcggcctg gttcgcgccg aacagcggac
     1141 atccgcgtcg cgcgcacgcc gcgtgtccgt gcacataaat cagctcggat ttgcacagcg
     1201 cgcagaccat ggtgcaacgc tttagcactg gtccctgagt tatcgagggg cgtaatgaac
     1261 tcttgcaatt cagagaacaa caaatcttaa gtcgctaaaa atatcatcat tgatgcgctc
     1321 attgcaggtc gcatcaccac tgacttccgt cgcacttctc gtgaaggaac ggaacatgac
     1381 ctggcactgc tgttcgccat gagcaaaagg ttgttcaccg aacgcagcta ttgcgcatca
     1441 tactccaggg tgatttttgc cgcgtcgttc ggtgcgtgca cgatgttggg atgtgcaagc
     1501 aacgacagcc ccaacggtgg caccggaggg cgcagcgcct cgcaaggcgg tgctctcggc
     1561 agcggccacg gtggtaccaa cgcgagcggt gcttcggcga tgagcggcgg agcctctgca
     1621 ctcggaggta accaaggcac gagctcggcg acaggtggct cttctggaaa cggccagtct
     1681 gcgggcggca acgcgaacgg tggcacaacc ctcggttcgg gcggcaacgg cgccgtgtca
     1741 tcaggtggta ggtctacggc cggcggctcc ggaggcggca gctccgccca gggtggtggc
     1801 tcgaacgtga gcaccggagg caaccagggt ggtggcgccg cgggcgcggg tggaacgaca
     1861 tccaccggaa tgggcggagt cggtgggaac tacgtcggga ctaagacacc gggaacggcg
     1921 caaacgggag acgtcacggt cgaccccgcc aaaacccatc aagtcgtcga tgggttcggc
     1981 gaagccgatg tttggcaaaa ctcgtcgtcc gcggcaatgc aaacgctgct ttgggatccg
     2041 gtgaacggaa tcgggcttac cctgctgcgg atcggcatcg agagtaagtc gggtacgagc
     2101 ttgatcatgg gcgacgctgc gctccaagac ggccccgcct gcaagaagtt cgcgggagac
     2161 gactgcaagg tgtgggctgc cccttggtcg ccgcccgcat cgatgaagaa caacgacaac
     2221 gtcaacggcc ctggcggcaa caacagctgc tccgggacca atgacacgct caaggacggc
     2281 agttacgaag cttgggcctc ccttttggcg gcgttccctg ccctctacaa gcaaaagagc
     2341 ggcgtcgatc tctacgccgt ttcggctcaa aatgagcccg acttcaaccc gaactacgag
     2401 gcgtgttgct atgacaagaa ccaaatggtc aagctcatca acgcgttggg gccaaagctc
     2461 gcggcgctcg acccgccggt gaaagtgctc gcggcagagc ctgacacttg gggcaatctt
     2521 tggggcggtg accagtacgg ccccgctatt ctcgccgacg ccaaagccag ctcctacgtt
     2581 gggcccatcg ccactcacga ctatgggagc tccggcgtca cgcgaccatc ccctccgtcg
     2641 aacaacaccc accatgtttg ggagacggag tgcacgccga gcgataccgg cccgatcacg
     2701 atcgcgacga tgatctacgc cgcattcacc agcggcggcg cgaacgcttg gcactattgg
     2761 tggacgcagc agctcgtgcc cgacgtgaag agcccgcccc cacaagtcta cgcgctcggt
     2821 aacttcagca agttcgtgcg ccccggctac tatcgcgtcg acgtatccgg cgcacccacg
     2881 ccctccacgt ccaatccgct ggtcgtcgca ttttccaatc tgagggacgg gacggttgcg
     2941 attgtcgtcg tcaacggtgg cagcgcccgc acgctctcgt tcttcgtcgc gggagcggca
     3001 tggccggcat cggttactcc ctacgtcacg accacgagct cgaagttggc tgcaggtacc
     3061 gccatcaacg tgaccgccgg ccgtttcagc gcgtctcttg ccgcgcagtc cgtcacgacg
     3121 ttcgtcggct cgccctagcc ccgcgcccgc gcgaagacga aacgttagcg ctcgcgcgaa
     3181 gacgaaacgt tagcgcccgc gcgaagacga aacgttagcg cccgcgcgaa gacgaaacgt
     3241 tagcgctcgc gcgaggcgaa aagttggcgc ccgcgcgagg cgaaaacgtt agcgcgtccc
     3301 gcaagcccag gcggggaagg ttttgcagcg cggcgcgtag aacgggtcac tgtctggctg
     3361 gtggggtcag acgtgagtga aatcaggttg gtttgggagg cagaatggga cgacagcaat
//
LOCUS       DASTKU010000393        31870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_295999, whole genome shotgun sequence.
ACCESSION   DASTKU010000393 DASTKU010000000
VERSION     DASTKU010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 31870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31870
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295999"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VFQ61_13515"
     CDS             complement(<1..803)
                     /locus_tag="VFQ61_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955524.1"
                     /translation="MAPASKLVEIHPASPQASYIDGNSEGTQGPLVSEDFVKDTRLRH
                     DRPTLPTSGVGREELRASALKPFLLRLKDERGDAAVRALLATSGIPYSVIEGDVGWVS
                     VAAARRALSALATALGPKSLESRGDWMTHPEVLGAYVRLLRSANNPKDAFRYLALNAA
                     ENTRVGTYELDVVSDGHAEIRYRPRPDAECEQNDPLLCSVRRAELVSIPKIWGLAEAS
                     ISHPRCIALGADECRYVLDWVAPRRPHWIAFGGLLGAASCGGAVSLSGS"
     gene            876..3143
                     /locus_tag="VFQ61_13520"
     CDS             876..3143
                     /locus_tag="VFQ61_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9955525.1"
                     /translation="MLAVDTWQVSSLSAARRQSASRIPVLHISAQNLRRLFLLLPIAW
                     LGLILLAAASVRWRAWPEEHRHGAPPPSASGLPTATSAPARATSFIESRRADAKILNP
                     LLQADSASNDLMQRRIFESLVDNDEQQRLVPRLAEAWQVTEKAYVWELPERRLANGSP
                     ATLENLRLALTSAAVPRAVASEIQLIDDSPAQERSFREEVLLDVKQTSGELTGRVRVP
                     RRVRLQLKHVVPDLFVQLAGLLGPDYPCPGSIDTAITLDHDPFGDRERALTALGKDRH
                     QLASPCEHNPILSFDLRKNVRFHDGVPFTSADVVFTYRALVDPKNNSPRISNYAPIRS
                     AVALGPHRVQFEYSRLHSTALLDWQMGIVPEHLLNARVLAREAERLAPAARARFSLRD
                     ASFNRHPVGTGPFRFVEWKSQQYAHVTRFDDYWGERPQLRDIYTRVLPDPLTEDLELQ
                     AGAIDSFEVLPYQVARFSHDPKFRLLTGAEGHYSYIAYNLQRPALQDVRVRRALGMAI
                     DVDAIIRYVLHGQGRRSSGPFYSHTPFADPTTPLLPYDPKGAAELLKAAGFRKNRQGW
                     FEKDGRVLEFTLLVNNGNPQRKLVATIAQEAWAKLGVRCTVQAFEWAVFLRTVMEAHD
                     FDAIVLAWVGADLSPDRFEIWHSSSVRGGQNMIGYSNPEADRLMEAIRSEYDRDQQIR
                     LARALHRTIAADQPYTFLYEPLTSRAVPKDLVMADPADPSRLVPVQASRAGEPLFHLN
                     AWRRRPAPASELVSQ"
     gene            3166..4128
                     /locus_tag="VFQ61_13525"
     CDS             3166..4128
                     /locus_tag="VFQ61_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9955526.1"
                     /translation="MWSYLLRTILQRGLTLIFISIVSFSLVHLAPGTPSEIDPMNPRM
                     RAEDVVRLRKAFHLDEPLYRQYALWVSDLGRGELRSFRDGAPVMSRIGERFRNSLPLF
                     AASTLLIWTLSFPLGIHAAVRRGSRFDRTTTVLAYALISTPSFFIAYLAVLIAVRVLG
                     LPVLGMSTFGVNYGSATRALLDRIWHVALPAGLAALSGVAVLSRYVRSQMLEVLTQDY
                     VRSARARGLPEDQVVYGHALRNALLPFITMFGLMLPGMLGGSVIIEQIFAWPGIGRLG
                     YEAIVARDYPMILTLNFLVAALTLFGVLLSDISYALADPRIRLS"
     gene            4146..5048
                     /locus_tag="VFQ61_13530"
     CDS             4146..5048
                     /locus_tag="VFQ61_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9955527.1"
                     /translation="MPTSSEPHFSPAREALRRFRGNGLAMLGYWGLLAVGAFALLGKF
                     LTEWWIVFDPARVRLEHKLLPPLSMRGEFFLLGTDELGRSLFARLLQGASVSLGIGLV
                     AMAISISVGILMGGIAGYYGQRKLAYIAIDSWVMRLTDVALCFPSFFLILTVIALLPS
                     SIFHVMVVIGLTHWMGTARLVRAEFLALRELDFVTSARALGFSDRRIIFRHILPNALA
                     PVFVTANIDVARAILTESALSFLGFGVDPPHATWGNILADGRAYILDAPWLFLIPGLA
                     ILGIVLAFNLVGEGLREAFHTKAR"
     gene            complement(5187..6230)
                     /locus_tag="VFQ61_13535"
     CDS             complement(5187..6230)
                     /locus_tag="VFQ61_13535"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9955528.1"
                     /translation="MTGTASGSRSQSPAPQTAESVTGRVTTSTASDISPYHPGESELQ
                     TRVGVREQAERMGRKLIRSYMPDEHQELFEKLNFIVVGSVDERGQRWASILVGVPGFM
                     RSPDPSTLRIDALPSSFDPLSSALRPNAPLGLLGIEFATRRRNRMNGWISECDEGGFA
                     VRVLQSFGNCPKYITPRKSVRVERAPQAPQREGPVLSAQARALIAEADTCFLASASLP
                     VDGTPSQASPPHGVDVSHRGGPPGFIRVRDTWAEGSNGAADVLMMPDYRGNNAFNTLG
                     NLLLEPGAGLTIFHPERGDLLSLTCRAELIWDGPELERHPHAQRVLALHVVDGVWVPG
                     ALPLRFDLIGGAP"
     gene            complement(6227..6874)
                     /locus_tag="VFQ61_13540"
     CDS             complement(6227..6874)
                     /locus_tag="VFQ61_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HET9955529.1"
                     /translation="MTTKSSSLVSDSASAIVLHRFGLSGHAHRVQLFLSLLGLPHRIV
                     EVNLLAGEHKKPEFLKKNPLGKVPVIEDGELTLWDSNAILMYLAQRYDRSRTYWPEEP
                     VAAARVQSFLSLAAGPLAYGPAALRVHALFKSRLDVEAAMTTTTNLFGYFENILSQQR
                     FLSAPHPTLADVACYTYTAHAPEGGFSLEPYPAVRAWLTSVEALPGFVPMQRTPS"
     gene            6956..7864
                     /locus_tag="VFQ61_13545"
     CDS             6956..7864
                     /locus_tag="VFQ61_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9955530.1"
                     /translation="MDHLESLRLFVKVAETGSFSQAARRAGCSASAATRAIGSLEARL
                     EAQLLHRTTRRVSLTEGGERFLQDCRRILLDLERAEASVSRAAEELSGPLSVTASVMF
                     GRLFIAPIAVSFLELFPKVQLRLMLVDHLVDMMAEGIDVAVRIAELPDSSLSATRVGS
                     VRRVVCASPDYLAQHGEPRSPADLANHEAIQYAGGGPQRWSFHWKNRTRVVSPKMRMT
                     VNSTEVATQFAVAGLGLTRALVYQLVPELRAGKLKIILTKYEPPAVPIHVLHRERPRT
                     SHKVRAFVDLVVQRLRAEAYLTEPPR"
     gene            complement(8059..8949)
                     /locus_tag="VFQ61_13550"
     CDS             complement(8059..8949)
                     /locus_tag="VFQ61_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9955531.1"
                     /translation="MGCRKDNADKPHPVESGPNKQSIKQNYEGLKTQVSALGTKLTSL
                     HKQLEELPQEIPAVSEMRAKLFAAEEVLGVTGAKLSWLAERVDANGSAGTVEDLRQLS
                     KEISDTGKEIQEVDRVGLELTHQLLPFRKMLASGAKDAAGGPAFARRLSTGYEIQGRK
                     DGLEQRLVEQIEDSKKKADQKSWFDLDRVVFHAGGADLDREQSKAQLQNLAEILKAYP
                     SVKLRIGSHTDNSGAAAGNKKLSVERAQAVKKELFGFGVAAARLETEGFGAEHPVCPA
                     NDTEECRARNRRTAVVVIAK"
     gene            9420..10940
                     /locus_tag="VFQ61_13555"
     CDS             9420..10940
                     /locus_tag="VFQ61_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET9955532.1"
                     /translation="MTRYRNSFLVSTILSLGLFAVIPARAASVTQVSRSTWGASGVPN
                     YVNMYIYVPDKLAANPPVVVACHSCGTPVSGYVNSISGIKAAADKNGFIIILPEATGQ
                     NCWDVGSKKSLTHDGGGDTQAVAQMVKYVLTKYSGDAARVYAMGGSSGAMMTQALMAV
                     YPDIFKAGSARAGVAAGCWSDGYDTSNQWSNNCAGGRTTKTAQQWGDLVRGMNQGYSG
                     PRRRIQLFHGNADATINFNNLGESVKEWTNVLGLSDMPTTSDSVTTSVATYKRRLWKN
                     TCDFTVLEAWEGQNGSHSMAYETDAILSFFGLDKAGGADPEQPCSGNGGSGGAGGTAG
                     ASSGGVGGNAGGASTAGGTSGGGALGGGGASSGGTAQAGRGGTSSAGASNSMGGVNAS
                     GGVVNSGGASSTQAGGMNSSNSGGLPAGGMSGTGGNPNATGGASPGGHPSGASGQATG
                     GAATASGGASNPSQSDDGCSVTGPTPKTNSVLSSAFALGLAVAGLRRRRQRGSHRI"
     gene            complement(10946..11836)
                     /gene="bla"
                     /locus_tag="VFQ61_13560"
     CDS             complement(10946..11836)
                     /gene="bla"
                     /locus_tag="VFQ61_13560"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HET9955533.1"
                     /translation="MLTRRLFMAASLFFATSAGCKRTSQGPAANSELPFGEALRRLET
                     AAQGKLGVYVIDTASGREFGHRADERFSMLSTFKLLASAYVLARVDRAEESLPRRVQY
                     TRQDLVDWSPVTEKHLEQGELSLEELCHAAMTTSDNTAANLILRSFGGPPALTRFVRT
                     LGDDVTRTDRFETELNTPSNQEPLDTTTPRAMARTIHRLVLGDVLSPKSREILQSWLL
                     ANTTGLKRLRAGLPASWKLGEKTGTTPRATHDVGVAWPPAHAPVVVTTYLASDTAAND
                     VREQTLAEVGRLLPALIRAT"
     gene            11933..12799
                     /locus_tag="VFQ61_13565"
     CDS             11933..12799
                     /locus_tag="VFQ61_13565"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9955534.1"
                     /translation="MDLPLNALRAFEVSARHLSFTAAARELHLTQAAVSQHVKNLESE
                     LGVLLFRRVPRGLALTDEGAALVPVLTESFERMAAVLDGIRGGQKREVVSLGVVNTFA
                     AGWLIPHLREFQQRYPLVDLRLFSNNNRVDLAAEGLDYAIRFGDGHWESVHAKLLLHA
                     PLTPMCAPALARRVRTPRDLARESLLRSYRAEEWETWFRAAGVEPPRLTGSRFDSSLI
                     LANAAENGAGIALLPARLFRREVHEGRLTQLFDIEVDAGSYWLTRLKTRRTTPAMATF
                     SQWILQEAPRST"
     gene            complement(12868..13257)
                     /locus_tag="VFQ61_13570"
     CDS             complement(12868..13257)
                     /locus_tag="VFQ61_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955535.1"
                     /translation="MPSAVEVAVETYIRAVGERDSVLRARLLEACFSADCRMVTQSRV
                     VRGPAEIHREIERFLTDPQLVRARVVSAIDARGSIFRYRTIVERADGPSLEFFDAGEI
                     DAEGRISLLLAFAGPLGEAEQPRELLR"
     gene            complement(13520..14209)
                     /locus_tag="VFQ61_13575"
     CDS             complement(13520..14209)
                     /locus_tag="VFQ61_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955536.1"
                     /translation="MKLMGMASAGILMTLLGCSGQSSDDGKTGTLALNLIGTDRAGEQ
                     YRLRNASFDLYGFPDYFEVVGKDEPPPPMGTGGAAGGGMTTPSAGFAGSVTGGSANYY
                     SESFSSEEDPDSATISRRLVPGYYYLTLSNSDFYMEHLTPSGPERVEKLVLLSGRTTS
                     AYIWNGGVTDVAYQFGINGDLIDFRSGELRVNIGIEHPGENMGTGGYPIGGFTSTGGS
                     AGAAAGGRASF"
     gene            15025..16611
                     /locus_tag="VFQ61_13580"
     CDS             15025..16611
                     /locus_tag="VFQ61_13580"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9955537.1"
                     /translation="MAYTISSSSSVHASWPEASLIPAIWSAQDNRSQYILERSGPAPA
                     PAEVELEHVTALEVIMLWGSQVLRVEHLNPPRNVSLGDRPLAANSAATRGTDFYMPSE
                     ALGRSSAEIVKVEGDQVHCSIFPGMRAFLEERGALREIELSHDLPVDVEQPEARRLSL
                     RSGQRVTLKIADFSLQIAPVSAGKPVDRGLRAAWNGAAAGYFGLSLGVHAGLLAAFAF
                     FAPPLGLSDADELDRDRLYVMQSYLDAAAERERLQEETPEVAAASKADGGTGSRSTGE
                     EGSMGNPTSRATQRRYAVKGPRDNPDPTLAREQAVKEAAQFGVIGLLSAGLAGDPNSP
                     TAIWGADHASGRDDLSARGNIWGEEIGEAFGAGGLSLTGVGEGAGGPGKGVGFGDVGT
                     IGHGRGLGDGQGFGNSVGGPGSRGHVGRVPTMHPGVTSVSGRLPPEVIQRVVRQNFGR
                     FRMCFNEGLRRNPNLEGRVAARFVIDRSGAVSAVSNGGSDLPDSAVVSCVLSAFYGLS
                     FPEPQNGIVSVVYPISFAPG"
     gene            16645..17505
                     /locus_tag="VFQ61_13585"
     CDS             16645..17505
                     /locus_tag="VFQ61_13585"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9955538.1"
                     /translation="MQIVILGAGFTGTAVTELALARGLRVLATTRDAERAHQLEQRGA
                     QVLLAPALPASDLLRQVSSESRVLVTFPPDGHTDAALAEALSVCARIVYISSTAVYGA
                     HTGLLDETTPVLPNTPRGERRFAAERVWHDAGATILRAPAIYGPGRGLHLRLTRGDLK
                     LGPNPTNTISRIHRDDLALAALEMLGRKLEHSTYLIGDLEPAPHLEVVNWLATELRLP
                     APVPDASVTPDETLTSDRQVNALRLWHELELRPRYPTYREGLRHCIRVDLPNWVGAAS
                     SDTVHSEGNG"
     gene            complement(17502..20429)
                     /locus_tag="VFQ61_13590"
     CDS             complement(17502..20429)
                     /locus_tag="VFQ61_13590"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9955539.1"
                     /translation="MITQSPSRSQRKAIGVLIDHADYLSAGYETQLRSGFHSACQEFG
                     LDLFFFFGRPLEDPGYRAQNSIYDLAHTDCLDGVIAVSPGLAAFTGAPGVQALLEKMV
                     GLPRCSLGMVIPGIPSVVADNTPGMRAAVEHLIDVHGCRRLAYLGGPSGNPDASLRRQ
                     VFLQVLAERGIVHDPALETECLFTLPRGIEAMREILSRGVAFDAVVGANDGIALGAID
                     ALKAAGWRVPRGVRVTGFDDAPLARICNPSLTTVRQPLVEMARCAVATVRAGILGQQV
                     PDQRVLPVTFVARRSCGCFEDMATRSKRSHGHDLDPDVRIRRVASCLKGLGSSAAVTA
                     EALVRAEQPIHVRDWLEDWMAQAAETSVDYDELDAALAELRRTAPSEYRHDDGWFEVF
                     ALTARERTYAQARNRAQADVGYWHLLRGGERLLTAFELSEVAHILATSLPRMGVHNAQ
                     LSLYTDDSRRQLRSFLCLRGGKAQAVREQPYAATELVPDLVGEDADSGTYFVWPLAVE
                     ADQFGIFVLQVDPRAAPHEMLREQVSAALKSVKLQREIIETTAAHERGTQERLATARR
                     MHALNVLAGGVAHDLNNSLGPLVGLPDVILRELEGSLGERSPTPLEVPEVRADIAAIK
                     TAALRAAQTIKDLLTSARQGRTSKDSLDLNRVVVACVQQERSHQAEGSISTQPFRKAG
                     RPEDAIHGVEIVHSLWPDALIVKASEAHLLRAISNLLRNAREAIDGRGRVSVSTRGVV
                     LQGPLAGYETIAAGSYAVVCVEDTGRGIPQADLGRVFEPFFSKKRTGEHSGSGLGLSV
                     VHGVVKEHGGFLDVESTPGVGTRFTLYFERSRESLRPREEPPPSVRSRKVRIWVVDDD
                     ALQLRTAARVLSHQGHEVTTETSGRRACERFELASRQQRESPCDLLILDVQLGEDVDG
                     LGVLEKVQRLFPEQRAIMVSGHATLPSTIESNSRFAAWLPKPYTLDQLGRAVQSALAD
                     AVV"
     gene            complement(20730..22175)
                     /locus_tag="VFQ61_13595"
     CDS             complement(20730..22175)
                     /locus_tag="VFQ61_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET9955540.1"
                     /translation="MVDAQAAHRTLALSALLVIALPGCLKTPDHVAASTLDAGLNPPA
                     NFELGGRPFCFAGANNYYLGYKPSASVDALLDSARALGFAVVRTWAFIDIGSLDGNVR
                     HTDPNADGKKDGVYYQYWDTKALRPAYNDGENGLRRLDYAVAKAAERGLKLILVLTNN
                     WEAFGGMDQYLAWFGRTKHHEFYTDPLVKQAYKNYVAHLIARKNTYSGKLYRDDPAIF
                     AWELANEPRCKGSGPGASGWTNQTIVTWADEMSAHIKSLDPNHMVSVGDEGFLDVGGE
                     HWAYKANDGVDHAALTALPDIDFGTFHAYPEDWGARLSWGEKWIADHARLARQLGKPT
                     ILEEYGVKVVRDDHGRIVKGLHDRLRAYERWNQLALRKGINGSMVWMLSDRDGPRLYE
                     DYDQYTVYRGDDTAKLLGAFASQFLTAAPACSAAGPSRDAPSEFVRVRRPALSNALAF
                     DGWAQPAAGHGRPSFPAGDRGFSMSFRRSLH"
     gene            complement(22296..22493)
                     /locus_tag="VFQ61_13600"
     CDS             complement(22296..22493)
                     /locus_tag="VFQ61_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955541.1"
                     /translation="MQARASDHDHGERDRIQLDRLDVTAASSLGLTSGMYERHAYEKR
                     RIELRLAAASAPMGQLTNRMT"
     gene            22861..23553
                     /locus_tag="VFQ61_13605"
     CDS             22861..23553
                     /locus_tag="VFQ61_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955542.1"
                     /translation="MRRASSTILLALALGACDATVEPIHVRSGCPARPNHEANTLARA
                     DIIDDFEDEDLDLLVANGRDGSWIVGWDMTSERPLTEGASTCAASGRAAGHFSASGFT
                     DWGANWTAVLRRESNGRAVPYDASDYSGISFWAAVGADAPVLTNLPVGVTTMDVAWNG
                     GVCSGTCMDYYRTTISVTRDWQRFVVRFEALAQRGFGDPLTALKRDQLVGFIVWPDRA
                     FDLWLDDVRFER"
     gene            complement(23605..24660)
                     /locus_tag="VFQ61_13610"
     CDS             complement(23605..24660)
                     /locus_tag="VFQ61_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955543.1"
                     /translation="MVCCVAAVWSLTTTAGAASEESGSSRVLVVESNRSSMLERVTAE
                     LTGLGFTVVRRAASGSLDDMARAEHASAAIRVLPSRKGVEVWMADATSGRSLLRQVIV
                     DEDPNGPNERLIALQTAELLRTSLLAQRDSASHELADPPKSELPPNQAQNAAVIPPEK
                     HRDDAPAASDSVAPRAGSFRESGMQAWLGALYSPGGAGAGLQLGLSLQHSLSERWGLL
                     LDLSAPLRSANISSVEGSASVGASFAGVGVFTRIRAANHPYIATLGAALALMRIGFDG
                     DARVPLQARDEHVVTGAAYLRADAAVEPLTWLRLGVRGTAGATAERVRVQFAGNDSGS
                     WGPGFFALFGLCEVALR"
     gene            complement(24705..26045)
                     /locus_tag="VFQ61_13615"
     CDS             complement(24705..26045)
                     /locus_tag="VFQ61_13615"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9955544.1"
                     /translation="MDSQKAGLTEVASKIQDALAAETHPSEQIRIARTRLLREVTTGQ
                     LARNSRGSSASVPRLGLWWVGAASGVAAIAAVLGWVLLRERPISFEIGAHPTTGQLGD
                     VVQANNGQAVPVRFSDGSSVVLAKEGRMRVLAADPSGARVLLEQGEVDVSVTHRAGKQ
                     TRWNFEAGPFQVHVIGTEFAMAWNPSLSKFSLRTRAGSVRVLGPCLDGPPGLQGSRAV
                     DAGARLEVNCPEAAPAERSDKFSLNTPAPGTAASAWSVDPGQASTLVRGRELEQVGTS
                     PEAQHPAVAPTSSLNSEPNWREALRSGRGADALRIAESSSFPAVCERATRSELLELAD
                     AARLSAKSQLATTALLALRKRFSGSTDAATAAFALGRIAFEQQHDYSRAARWFKAYMD
                     ELPGGPLIGDAAGRLMEAKIRQGDRAGARLEAERYLKRFPQGPYAAQARRISSE"
     gene            complement(26021..26632)
                     /locus_tag="VFQ61_13620"
     CDS             complement(26021..26632)
                     /locus_tag="VFQ61_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9955545.1"
                     /translation="MVTPLKFIGDEHALLEALRAGHPGAVAALYDQYSVHVHRTLRSA
                     LGPDADLPDLLQEVFIRALDHISEINDPLRLKAWLNTIAVFTARAHIRRRARRRWLSI
                     FSPQRLQEQELEPPCSDARFALRETYRLLDEMPVGERMAFVLRIIDGQTLPEAAAACG
                     VSLATFKRRLARAEKAFVERVSERPTLLTWLERGTRWTLRKQG"
     gene            27216..28832
                     /locus_tag="VFQ61_13625"
     CDS             27216..28832
                     /locus_tag="VFQ61_13625"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9955546.1"
                     /translation="MHQIKLGSSVPIALFLALTACSSEPNGGTTKPPGSGGQAGAPTS
                     GGSTATSGGKSASGGSGGASGGATNGSGGVSPSGGSTSGSGGTTVGGSMTAGGSAGTA
                     GVAAGGTQASGGANTSGGSSALGGTSSGGTGAIAGTSSSGGMAGSTGIAGASGTGGSA
                     GGGSMHWVGTWACGPQTTETNNLPPSPGLSGNTLRQYVHVTIPGKQLRIRLSNEFGTS
                     PVTFESVHVAASKGGGAIDTATDKTLAFSGMPNVTVPAGKTVLSDMFDYNLPALSDLA
                     ISIKFGTQSGDVTGHPGSRTTSYLQSGNAVSEASLASAAKTDHWYFITGVDVMAPESA
                     AAIVVLGDSISDGRGSTTNGNDRWPDALAKRLQANAATANVAVLNQGIGGNTVLAGGL
                     GPTAKARFDRDVLGQTGAKWLIIFEGVNDIGNNSTGNVANDLIAAFGQFIEKAHAKNM
                     KVFGAPITPFAGSQYDSSAHETTRQTVNQWIRTSGKFDAVIDLDAAVRDPNKQTALLG
                     SYESADKDHLHLGPAGYKAMADAVDLTLFK"
     gene            complement(29014..29829)
                     /locus_tag="VFQ61_13630"
     CDS             complement(29014..29829)
                     /locus_tag="VFQ61_13630"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET9955547.1"
                     /translation="MAFLDHPLMTEEFRIVAYRGGSAERPENTLEAFDRAASIAPSIV
                     LELDIRRTSDGVLVAAHDDDLGRTTGKPLCVSRISHAELAELDAGYGFERAGEFPFRE
                     RGSRVPRVEEVLSSFPNHCCVLDVHSDHPRVVREVVDLVKRQRAASRVVIASERPQIV
                     RELRKLQPGWLCCATSSELLSRVLLERVKLDAMAPRTGGMLMIPEMHRSLCVLTPRFV
                     QRARARNERIWVWVVEEVEEMRRLRGLGADGVFTPSPTQFWNATLAQGRSYPG"
     gene            complement(29987..31684)
                     /locus_tag="VFQ61_13635"
     CDS             complement(29987..31684)
                     /locus_tag="VFQ61_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HET9955548.1"
                     /translation="MTDASRLLSISSPRSTLSSRMAPMIAAAIAAAACSSQGGTPGAP
                     VDSGVSGGAAGGASGAAPHGGATQGSSAHGGSGNGGTGQPSGATGGVGGAGAGAAMGG
                     FTVNRQVPDWDAELRLPSAQDSNSDPHVFETTLDARVSEIEVVPGKRTPFWSYSGVLP
                     GPFIHVRRGDRAIIHFKNELPEPTTIHWHGLRIPSEMDGTPGHSQPEIEPGQSFTYDF
                     VVPDAGLFWYHPHVNSAKQVGNGLYAPFLVDEEEDKNVTAKLGDELTLVLSDASISES
                     GALEPADSGGDLGTLFGREGNTLLVNGKVQPTLKVRSGLLQRWRIVNTSRARYYQIAL
                     KEHRFRRIGGDGGRMEAPVDSDMVVVVPAERSDVLIVPVGEPGTRVPVRWVPYDRGFG
                     TTFARPEQDLFYIEFVGDPVEPPEALPSPQRSIEALDLQGATPVEMELTQNSGTGEPL
                     ELGINGIPASRAVPLQAALGETQIWTITNPTIDFAHPFHLHGYFFQVLDEDYRPVHPL
                     EWKDTADVPVKGTLRIGVRFDDRPGMWMYHCHLLDHADAGMMGMVEVGSEMSGHDMH"
BASE COUNT         5963 a        10409 c         9641 g         5857 t
ORIGIN      
        1 taactcccgg acaagctcac cgcgccaccg cagctcgccg ctcccaacaa tccgccaaaa
       61 gcgatccaat gtggccggcg gggcgccacc cagtccagca catagcggca ctcatccgcc
      121 ccgagcgcga tgcacctcgg gtgcgaaatg ctggcctcgg cgagccccca gatcttgggg
      181 atggaaacca gctcggcccg ccgcacgcta cagagcagcg ggtcattctg ctcgcactcc
      241 gcgtccggcc gggggcgata gcgaatctcc gcgtgaccat ccgatacgac gtcgagctcg
      301 taggtgccca cacgcgtgtt ctccgctgca ttcaaagcca agtagcggaa cgcatccttg
      361 ggattattgg cagagcgcaa cagacggacg taagcgccca aaacctccgg atgagtcatc
      421 cagtctccgc gcgactctag agatttgggc cccaaagccg tggcgagagc ggagagcgcg
      481 cggcgcgcgg ccgcgacgct gacccaaccg acgtctccct caatcacgct gtatggaatt
      541 cccgacgtgg cgagcagcgc gcgcaccgcc gcgtctccgc gctcgtcttt gagccgcaac
      601 aggaacggct tcagcgcgga cgcgcgcaat tcctcgcgtc caacgccgct cgtcgggagg
      661 gtgggtcgat cgtggcgcag ccgcgtgtcc ttcacgaaat cctcggaaac gaggggcccc
      721 tgagtgccct cggagtttcc gtctatatag ctcgcttgcg gcgacgctgg gtgaatttcg
      781 acgagtttcg acgcgggggc catccaccgc tcccgaaggc ccaatccaac ctcttagact
      841 ccgcgtaaaa cctgggtagg acgcatggct acagggtgct cgccgttgac acctggcaag
      901 tctcgtcgtt gtctgcggcg cggcggcaat cggcctcacg cattcccgtg cttcacatct
      961 cggcccagaa tctccggcgc ctgttcctgc tcctgcccat cgcttggctc gggctcatcc
     1021 tgctcgccgc cgcgagcgtt cggtggcgcg cttggcccga agagcatcgg catggagcgc
     1081 cgccaccttc tgcttcgggc ctgcctaccg ccacgagcgc tcccgcgcgc gcgacgagct
     1141 tcatcgagtc gcggcgggcg gacgcgaaaa tcctcaatcc cctgctgcaa gcagacagcg
     1201 cctcgaacga cttgatgcag cgccgcatct tcgagagctt ggtcgacaac gacgaacagc
     1261 aacggctagt tccccgactc gcagaagcct ggcaggtgac cgagaaggcc tatgtttggg
     1321 aacttccaga gcgaagactc gccaacggat cacctgccac actcgagaac ctgcggctcg
     1381 ctttgacctc ggccgccgtt ccccgggcgg tcgcctccga aatccaactg atcgacgaca
     1441 gcccggcaca ggagcgttcg tttcgagaag aagtcctgct cgacgtcaag cagaccagcg
     1501 gtgagctaac ggggcgcgtg cgggtaccgc gtcgcgttcg cctgcagttg aagcacgtcg
     1561 tgccggacct gttcgttcag ctcgcggggc tgctcggccc ggattaccct tgccccggct
     1621 cgatcgacac tgcgatcacg ctcgatcacg atccgttcgg cgaccgagag cgggcgctga
     1681 cggcgctcgg aaaagatcgt caccagctcg cctcgccttg tgaacacaac ccgatcctca
     1741 gcttcgactt gcgtaagaac gtgcgcttcc acgacggggt gccgtttacg tcggccgacg
     1801 tagtcttcac ctatcgagcc ctggtcgatc ccaagaacaa ctctccccgc atcagcaatt
     1861 acgctcctat ccgcagcgcc gtcgcgctcg gcccccatcg ggtgcaattc gagtattcgc
     1921 gcctgcactc gacggcacta ttggactggc aaatggggat cgtccccgaa cacctgctga
     1981 atgctcgcgt gctggcccgg gaggcggaac ggcttgcccc ggccgcgcgt gcgcgttttt
     2041 ctttgcggga tgcctcgttc aatcgacacc cggtcgggac ggggccgttt cgcttcgtgg
     2101 agtggaaatc acaacaatac gcccacgtca ctcggttcga cgactactgg ggagagcggc
     2161 cgcagctgcg cgatatctac acgcgcgtcc tgcccgatcc gttgaccgaa gatctcgagc
     2221 tccaagccgg cgcaatcgac agcttcgaag tattgcctta ccaggtcgca cgcttcagcc
     2281 atgatcccaa gttccggctg ctgaccggcg ccgaagggca ctacagctac atcgcctaca
     2341 accttcagcg ccccgcgctg caagacgtcc gggtgcgccg cgcgctcggc atggccatcg
     2401 atgtcgacgc gatcattcga tatgtcctgc acgggcaagg ccggcgcagc agcgggccct
     2461 tctactccca tacccccttc gccgatccga cgactccgct gctgccttac gacccgaagg
     2521 gcgccgcgga gctgctaaag gccgccgggt ttcgtaagaa ccgacagggc tggttcgaga
     2581 aggatgggcg ggtgctggag ttcacgctgc tcgtcaacaa cggcaatccg cagcgcaagc
     2641 tggtcgccac catcgcccaa gaggcgtggg ccaagctcgg agttcgctgc accgtacagg
     2701 ccttcgagtg ggccgtgttt ctgcgcaccg tgatggaagc ccacgacttc gacgcgatcg
     2761 tgctggcttg ggtgggggcc gatctcagtc cggaccgatt cgagatctgg cactccagct
     2821 cggtccgcgg gggtcagaac atgatcggtt attccaaccc ggaagcggac cgcttgatgg
     2881 aggccattcg cagtgagtac gaccgcgacc agcagatccg cttggcgcgc gcgctccacc
     2941 ggacgatcgc cgccgatcag ccttatacgt tcttgtacga gccgctcacc tcgcgtgcgg
     3001 tcccgaaaga cttggtgatg gccgacccgg ccgaccccag ccggctcgtc ccggttcagg
     3061 cctcccgcgc gggagaaccc ctgtttcatc taaacgcctg gcgcagaagg cccgcccccg
     3121 ccagcgaatt ggtcagccaa tgaagcggcg cacccgaact tagcgatgtg gagctaccta
     3181 ctacgaacga tccttcagcg cggtctaacg ctgatcttca tatccatcgt gtcgttctcg
     3241 ctcgtgcacc tcgcaccagg cacccccagc gagatcgacc ccatgaaccc gcgcatgcgc
     3301 gccgaagacg tggtgcgcct gcgcaaagca ttccatctcg acgagcccct gtaccggcag
     3361 tacgcgcttt gggtctcgga cctcggccgc ggtgaactgc gctccttccg ggacggcgcg
     3421 cccgtcatgt ctcgcattgg ggagcgcttc aggaactcgt tacctctatt cgcggcctcc
     3481 accctcctga tctggacgct ctccttcccc ctcggcatcc acgccgccgt tcgccggggc
     3541 tcccggttcg accgcacgac gaccgtgctc gcgtacgctc tgatttccac gccgagcttc
     3601 ttcatcgcct atttggcggt acttatcgcg gtgcgtgttc tcggcctacc ggtactcggc
     3661 atgtccacct tcggcgtgaa ctacggttct gcaacgcggg ctctgctcga tcgaatttgg
     3721 catgtcgcct tacccgccgg actcgcggcg ctgtccggag tcgccgtgct ctcgcgctat
     3781 gtgcgctcgc aaatgctgga agtcctgacg caggattacg tacgcagcgc gcgcgcccgc
     3841 ggcttaccgg aagatcaggt ggtctacggg cacgccctgc gcaatgccct gctccccttc
     3901 atcaccatgt tcgggctcat gctgccgggc atgctcggtg gttccgtgat catcgagcaa
     3961 atcttcgcgt ggccgggcat tgggcgtctg ggctacgaag ccatcgtcgc gcgcgattac
     4021 ccgatgatct tgaccctcaa tttcttggtg gcggctctaa cactatttgg cgtattgcta
     4081 tcggacatct cgtatgccct ggcggatcct cgaattcgcc tgtcctaggg tgcggacgga
     4141 cgctcatgcc cacctcctct gagccgcact tctctccggc gcgtgaggcg ttgcgacgct
     4201 tccgcggcaa cggcctggcc atgctcggat actggggcct cctggcggtg ggcgccttcg
     4261 ctctactcgg caagtttctg acggagtggt ggatcgtatt cgatcccgca cgcgtccgtc
     4321 tcgagcacaa gctcctgcct ccgctctcga tgcgaggtga attcttcctc ctcggcactg
     4381 acgagctcgg ccgcagtttg ttcgcacgtc tgctgcaagg cgcgtccgtg tcgctaggca
     4441 ttggcctggt cgcgatggca atctcgatca gcgtcgggat cctgatgggc gggatcgctg
     4501 gatactatgg ccaacgcaag ctcgcgtaca tcgccataga ctcctgggtc atgcgcctca
     4561 ccgacgtcgc gctgtgcttc ccctcctttt tcctgatcct aaccgtcatt gccctgctcc
     4621 catccagcat cttccacgtg atggtggtga ttggcctgac tcattggatg ggcaccgcgc
     4681 gcctggtgcg cgccgagttc ttggcgctgc gggagctcga cttcgtgacc tctgcccgcg
     4741 cgctcggctt ctccgatcgt cgcatcatct tccgccacat cctcccgaac gctctggcgc
     4801 ccgtattcgt caccgcgaac atcgacgtgg cccgcgccat cctcaccgag tctgcgctct
     4861 cgttccttgg ctttggcgtc gaccctccgc acgccacttg gggcaacatt ctggccgacg
     4921 gccgcgccta catcttggac gcgccgtggc tgtttctcat acctggcctc gccatcctcg
     4981 gcatcgtgct cgcgttcaat ctcgtcgggg aaggcctacg cgaggccttc cacaccaagg
     5041 cgcgttgatg ctccacgtgc aacgcttatg tagctcgttt cgggccactt cataacgtgc
     5101 gcgacccgcg tcaggaaacc ctcgagcgca gcctccgcca cccaagacac gtatcgccac
     5161 cgcgccggcc ccccccaata acgcactcag ggggcgcccc caatcaaatc gaagcgcagc
     5221 ggcagcgcgc cgggaaccca aacgccatcc accacgtgca aagccaaaac gcgctgagcg
     5281 tgcgggtgac gttcgagctc cggtccgtcc cagattagct ctgctcgaca ggtgaggctc
     5341 aaaaggtcac ctcgttcggg gtggaagatc gtgagtccgg cgccgggctc gagcagcaag
     5401 ttgcccagcg tattgaacgc gttgttcccg cgatagtcgg gcatcatcag gacgtccgcc
     5461 gcgccattcg agccttccgc ccacgtgtca cgaactcgga tgaagccggg tggtcctcct
     5521 cgatgcgaga cgtccacccc gtgcggcgga gaagcctgcg agggcgtgcc gtccacgggc
     5581 aggctcgcgc tcgccaagaa gcacgtgtcc gcctccgcga tcagagcgcg ggcctgcgcg
     5641 gagagcaccg gcccttcgcg ctgaggcgct tggggggcgc gctctacccg aactgacttt
     5701 cgtggcgtga tgtacttagg acaattgccg aaactctgca gcacccgcac cgcgaagccc
     5761 ccctcatcac actcactaat ccaaccattc attcggttgc gacgtcgagt tgcgaactcg
     5821 atacccagta ggccaagcgg ggcattgggt cgcaacgcgc tcgagagcgg gtcgaagctc
     5881 gaagggagag cgtcaatccg aagggtgctc gggtctggag aacgcatgaa acccggcacc
     5941 ccgactagga tcgatgccca gcgctgacct cgctcgtcga cactgcccac gacgatgaaa
     6001 ttcagctttt cgaacaactc ctggtgctcg tcgggcatgt agctccgaat gagcttgcgt
     6061 cccatccgct cagcctgctc tcgaacgcct acccgcgtct gaagttcgct ctcgcccggg
     6121 tgatacggtg agatgtcact cgcggtggac gtggtgacgc ggcccgtgac cgactccgct
     6181 gtttgcggcg ctggtgactg cgatctcgag ccggaggcgg tcccggtcac gacggggtcc
     6241 gttgcatggg cacgaagccg ggtagggctt ccacgctggt gagccacgct cgcactgccg
     6301 gatagggctc gagtgagaag cccccctccg gagcatgagc cgtatacgta taacaagcca
     6361 cgtcggccag agtggggtgg ggcgccgata gaaaccgctg ttgactcaaa atgttctcga
     6421 aataaccgaa caagttggtc gtcgtagtca tcgctgcttc tacgtccagt ctggatttga
     6481 acagcgcgtg cacgcgcaga gcggccggtc cgtacgcgag ggggcccgcg gcgagagaca
     6541 agaagctctg cacccgagcg gccgcgacgg gctcttccgg ccaataagta cgcgaccgat
     6601 cataacgttg tgccaggtac atcaagatcg cgttgctgtc ccacagcgtc agctcaccgt
     6661 cctcgatcac cggcaccttc ccgagcgggt tcttcttcaa gaactccggc ttcttgtgct
     6721 cacccgcgag tagattcacc tccacgatgc ggtggggcaa gccgagcaaa gagaggaaca
     6781 actgcacccg gtgcgcatgc ccggacaatc caaagcgatg aaggacgatg gcagacgcag
     6841 agtcggaaac gagcgaggat gatttcgttg tcatgcccgt aagatttgcg cccaaaccgc
     6901 agtttgctca atacacacga ttgacaaatc attattgcac tgtacgcaag aatgcatgga
     6961 ccacctggag agcctgcggc tgttcgtcaa ggttgccgaa acgggcagtt tcagccaggc
     7021 cgcgcgccgc gccggctgct cggcgtcggc cgccacccgt gccatcggta gcttggaagc
     7081 gcggctcgag gcccagctcc tgcaccgcac cacgcggcgc gtgagtctca ccgaaggcgg
     7141 cgagcggttc ctccaggatt gccgacggat cctgctcgat ctggagcgag ccgaagcgtc
     7201 cgtatcgcgc gcggcggagg agctcagcgg cccgctatcg gttaccgctt cggtgatgtt
     7261 cggacggctg ttcatcgcgc ccatcgcggt cagttttctc gagctgttcc ccaaggtgca
     7321 actgcggctg atgctcgtcg atcacctggt ggacatgatg gcggaaggaa tcgacgtcgc
     7381 ggtgcgcatc gccgagctgc ccgactcgtc tctcagcgct acgcgcgtcg gctccgtccg
     7441 tcgcgtggtt tgcgcctctc ctgactactt agcgcagcac ggtgaacctc gcagtccagc
     7501 cgatctcgcg aaccacgaag cgatccaata tgcgggcggt ggcccacaac gttggagctt
     7561 ccattggaag aacagaacgc gcgtggtctc ccccaagatg cgtatgacgg tcaatagcac
     7621 cgaggtggca actcagttcg cggtcgcggg cctagggctc acccgcgctc tggtctatca
     7681 gctcgtgccc gaacttcgcg ccggcaaact caaaatcatt ctgaccaagt acgagccccc
     7741 ggccgtgcct atccacgttt tgcatcgcga gcgcccacgc acctcgcaca aagtacgggc
     7801 gttcgtcgac ctagtcgtcc aacgcctgcg cgcagaagcc tatttgaccg agcccccgcg
     7861 ctgagagcct tagcgtccgt gaaaaaccaa agtcactgaa tcggaatcga gaattcctga
     7921 tcttttccga gctcagccgt gcaaaggacc ggcggatact cgatgaacat taggcgcagc
     7981 tctcggtccg tactcgtcgg cgttctctgc aacaccttgc ggatgatccc cgcgttagag
     8041 ccggtcgcag aggcttacct acttggctat caccactacg gccgttcggc ggttcctcgc
     8101 cctgcactct tccgtgtcgt ttgcggggca caccgggtgc tcggcaccga agccctccgt
     8161 ttctagccgg gctgccgcta cgccaaagcc aaacagctct ttcttcacgg cttgggcgcg
     8221 ctccaccgag agtttcttgt tgcccgccgc cgcgccagaa ttgtccgtat ggctaccgat
     8281 tcgtagcttc acgctcggat aggccttcag gatctccgcg aggttctgga gctgggcctt
     8341 ggattgttct ctatccagat cggcgccccc tgcgtggaac accacgcgat ccaagtcgaa
     8401 ccaactcttc tggtccgctt tcttcttcga gtcttcaatt tgctcgacca gacgctgctc
     8461 gagaccatct ttgcgcccct ggatctcgta acccgtggat aaacggcgag caaaggctgg
     8521 gccgcccgcg gcgtccttgg cgccggaagc cagcatcttt cggaacggta agagctgatg
     8581 cgtcaactcg agcccgacgc ggtcgacttc ttgaatctct ttgccggtgt ctgagatctc
     8641 tttcgagagt tgccgcaaat cttcgaccgt gcccgccgac ccattcgcgt ccacacgctc
     8701 ggcgagccaa ctgagcttcg cgccggtcac gcccaatact tcctccgccg cgaacaactt
     8761 ggctcgcatc tcgctgacag cgggaatctc ctgcggcaac tcttcgagct gcttgtgcaa
     8821 gctcgtcagc ttggttccga gcgcactgac ctgagtcttg aggccttcgt agttctgctt
     8881 aatcgactgc ttattgggcc cggactccac ggggtgtggc ttgtccgcat tgtccttgcg
     8941 acaacccacg acactactaa tcgtgatcaa aagacccagg gaaagaggca gtactcgcca
     9001 gttgaacccc gcaaaagcgc attctccggg gagttgcctc aggaagtgga atcgggatcg
     9061 aaccgtgaca tcagggacca ttgcttgccg cacgttagcc tatgtatcgg aagctcaacc
     9121 cgtatttgga gagttgcacg gggaatgcga gcatgagagc gctcgcaccc agccccgaaa
     9181 atcttcggca aagatcacct ctgaccgcaa ccttcggccg cgggcggatg acattttctt
     9241 gaacaccacg gagtatcacg aaaaatggcg gaacccgggg gaattcgcac caatgacctc
     9301 ctggcgcctc ctatcgccag aagtcgggtt tccgaaatga ccccaagccc gggcgggcat
     9361 tatgggaaac cgagcccccg ctgggaagcg tcgggaccgg tttggagagg tacgcggaca
     9421 tgactcgata tcgaaattct ttcttggtga gcacgattct ctccctcggt ttgtttgcgg
     9481 tcatacccgc acgagccgcg tcggtaacac aggtgagccg ctccacttgg ggcgcgagcg
     9541 gcgtgccgaa ctacgtcaat atgtatatct atgtgccgga caaactagcc gcaaatcctc
     9601 cggtggtggt ggcctgccat tcgtgcggca ctcccgtgtc cggctacgta aacagcattt
     9661 ccgggatcaa ggcggcggcc gataagaacg gcttcatcat catccttccc gaagccaccg
     9721 ggcaaaactg ttgggacgta ggctccaaga agtcactgac gcacgacggt ggaggagaca
     9781 cccaggccgt cgcgcagatg gtcaaatatg tgctcacgaa gtacagcggc gacgctgccc
     9841 gcgtgtacgc gatgggtggc tcgtccggcg caatgatgac tcaggcgctg atggccgttt
     9901 acccggatat cttcaaagcc ggctcggcga gagcgggagt tgcagcgggc tgttggtcgg
     9961 acggctatga cacgagcaac cagtggagca acaactgcgc aggcgggcga accacgaaga
    10021 cggcccagca gtggggagat ctcgttcgcg gcatgaacca gggctactcg ggccctcggc
    10081 gccgcattca gctgtttcac ggcaacgctg acgccaccat caacttcaac aacctcggtg
    10141 agtcggtcaa ggagtggacg aacgtgctcg ggctctctga catgccgacc accagcgaca
    10201 gtgtcaccac ttcggtagcg acctacaagc gccggctatg gaagaacacc tgcgacttca
    10261 ctgtgctcga ggcttgggaa ggacagaacg gtagccactc gatggcgtac gaaacggacg
    10321 ccatcttgag cttcttcggt ctcgataaag ctggtggtgc cgatcccgag caaccctgtt
    10381 caggcaacgg tggctcgggc ggagcgggcg gtacggctgg tgctagctcg ggaggcgtag
    10441 gcggcaacgc gggtggcgcg tcaacggccg gaggcacctc cggcggaggc gctttaggcg
    10501 gaggtggtgc ctcgagtgga ggaacggctc aggccggcag aggtggaacg agcagcgctg
    10561 gcgctagcaa ctcgatgggt ggagtcaacg cctcgggggg agtcgtgaac tccggcggcg
    10621 ctagtagcac ccaggcgggc ggcatgaaca gcagtaactc cggcggctta cctgccggcg
    10681 gcatgagcgg caccggcggc aatcccaacg cgacgggggg cgcgtcgccg ggtggccacc
    10741 ctagcggcgc gagcggccaa gctacgggag gcgctgccac agcctccggc ggagcttcca
    10801 atcccagcca atccgatgat ggatgtagcg tcaccgggcc tacgccgaag acgaattcgg
    10861 tcctgagcag cgcctttgca cttggcctcg ccgtggcggg cctacgtcgt cggcgccaac
    10921 gcggttcgca ccgaatctag acgcgtcagg tcgcacgaat caacgcggga agcagtcgtc
    10981 cgacttcagc gagagtctgc tcgcgcacgt cgttcgcggc ggtgtccgac gccaagtagg
    11041 tggtgaccac cacgggcgcg tgcgcaggag gccaggccac gccgacgtca tgtgtggcgc
    11101 gcggggtggt ccccgtcttc tcacccagtt tccagctcgc ggggaggcca gcgcgaaggc
    11161 gcttgagacc ggtcgtgttg gcgagcaacc agctctgcaa tatctcgcga gacttgggcg
    11221 aaagaacgtc tcctaacacc agccgatgga tagttcgggc catggcccgg ggggtagtcg
    11281 tgtcgagcgg ttcctggttg ctcggggtgt tcaactctgt ctcgaagcga tccgtgcgag
    11341 tcacgtcgtc ccccagcgta cgcacgaagc gggtcagagc gggggggcct ccgaagctcc
    11401 tcaagattag gttcgcggcg gtgttgtcgc tcgtagtcat tgcggcgtga cacaactcct
    11461 cgaggctgag ctcaccttgt tctaggtgct tttcggtgac tggagaccaa tcgaccaagt
    11521 cttggcgggt atattggaca cgccgcggca aagactcttc cgcgcgatcc acacgcgcca
    11581 gaacataggc gcttgccagc agcttgaacg tgctcagcat cgagaagcgc tcgtcagcgc
    11641 ggtgaccgaa ctcgcgccca ctcgcggtgt cgatgacata gacgcccagt ttgccctggg
    11701 ccgcggtttc gagccgacgc aaggcctctc cgaagggcag ctccgagttg gcggcaggcc
    11761 cttgactcgt ccgtttacag cccgcggaag ttgcaaaaaa cagcgaagca gccatgaaca
    11821 atcggcgagt gagcatgtga actcctttga atgcgcaacc tatcgggccc gtgcgcgccg
    11881 aagcaaacgg gattaatcga tagctgacca tagaaaatct tgggtatcgc tgatggacct
    11941 gccgttgaat gcattgcgag ctttcgaagt ttcggcccgt cacttgagct tcaccgccgc
    12001 cgcccgggag ctgcacctga cccaggccgc cgtgagtcaa cacgtcaaga atctggagtc
    12061 cgaactcgga gtgctgctct ttcgacgcgt gccgcgaggt ctggcgctca cggatgaagg
    12121 agctgcgctc gttcccgtat tgaccgagtc attcgagagg atggcagcgg ttctagatgg
    12181 cattcgcggc gggcaaaagc gtgaagttgt ctcgctcgga gtcgtcaaca ccttcgccgc
    12241 aggttggctc atccctcacc tgcgggagtt tcaacagcgc tatccgctgg tcgatctgcg
    12301 cctattctcg aacaacaacc gcgtggacct cgccgcggaa gggctcgatt acgccattcg
    12361 attcggggat gggcactggg agtcggtgca cgcaaagctc ttgctgcatg cgccgctcac
    12421 gccgatgtgc gcccctgcct tggcgagacg agtccgcaca ccacgcgacc tcgctcgtga
    12481 gtccctgttg cgctcctatc gcgcggagga atgggaaacc tggtttcgag ccgccggggt
    12541 cgagccgccg cggctgacag ggtctcgttt cgactcatcg ctaatcctgg ctaacgcggc
    12601 ggaaaacggc gccgggatcg cgctgttgcc agcccggctg tttcgtcgcg aagtacacga
    12661 aggtcggctc acgcagctgt tcgacatcga agtggatgcg ggcagctact ggctcactcg
    12721 cttgaagacg cgccgcacga cccccgcgat ggccacgttt tcacagtgga tcttgcaaga
    12781 agccccacgt agcacgtaaa ccaaacccgg cgtgggacca acgccgtgag aatgcgtgac
    12841 tgattagctc cgatacggag cggagcctca gcgcaagagc tcgcggggct gctccgcctc
    12901 cccgagtgga cccgcgaacg ccaagagcag cgagatacgc ccctcagcgt caatctcgcc
    12961 agcatcgaag aactcgagac tcgggccgtc cgcgcgttcc acgatcgttc gataccgaaa
    13021 gatgctgccg cgggcgtcga tcgcgctgac cacgcgagcc cttactagct gtggatcggt
    13081 caagaagcgc tcgatctccc ggtggatctc cgccggcccc cgaactacac ggctctgtgt
    13141 caccatccgg cagtcggcgc taaaacacgc ctcgagcagg cgcgcgcgca acaccgaatc
    13201 ccgctctcct accgctcgaa tatacgtttc taccgctacc tcgaccgcac tcggcatgac
    13261 gcatctttcc atgcggtccg ccctcgcggc aagcttcgaa ctttgcctga ccactgtgac
    13321 aagatggtag caaccgacgg tgtcggcaga gccggtgtcg gcagagccgg tctcggcacg
    13381 agcagaaggc tcaccgttga gtcagcgaca gcccttcccg gcgccatcca attcattgca
    13441 gggcatcgag tcggccgcgc tcctccggcc acccaaaccg cagctcgccg cggccgactc
    13501 gtattcaacc tgaagcaact cagaaggaag cgcggccgcc tgccgcggcc cccgccgaac
    13561 cacccgtgct ggtgaatccg ccaatcggat aaccgcccgt acccatgttt tctccggggt
    13621 gttcgattcc gatgttcacg cgcagctcgc cgctacggaa atcaatcaag tcgccattga
    13681 taccgaattg gtaagcaacg tcggtgaccc cgccattcca aatgtaagcg gacgtcgttc
    13741 gaccggagag cagcaccagc ttctctacgc gctcgggtcc gctcggcgtc aggtgctcca
    13801 tgtagaagtc ggagttcgag agggtaagat agtagtaacc gggcacgaga cgacggctaa
    13861 tggtcgccga atcggggtct tcctcgctcg agaacgactc tgaatagtag ttcgcgctgc
    13921 cacccgtgac ggagccagca aacccggcag atggcgtcgt catgcctccg ccagccgcgc
    13981 ctcccgtgcc catgggaggt ggcggctcgt ctttcccgac tacctcgaag tagtccggga
    14041 aaccgtacag atcgaaagag gcattgcgca atcgatactg ctcgcccgcc cgatcggtgc
    14101 cgatcagatt gagagcgagc gtacccgtct ttccatcatc cgaagactgc ccgctacacc
    14161 cgagtagcgt catcagaatc cccgcggatg ccatacccat cagtttcatt cgaacctcca
    14221 gtaaacttaa ggaactgctg aacccgttcc tgcactcgag cgaatgcacg aacgctcgct
    14281 gcgcgtaccc agaccggtgt caccgggcac gctcatcgga tcgtaggcac gccgcttctg
    14341 gtctgataac ggcgcgtcga ccccaccgtt gtgcaaggtc gatgccatga ccagcccaac
    14401 ctctttgggc ggtctccgcg agaaaatctg cggcacggaa agtcgggcgt cttgtgccac
    14461 gcgccggact gtgtcggctt gtgccaacga tggtcgctct ttcatccgtg cgcgccgtct
    14521 ctacccgagc ttccggctct tgtggcttgc tcatcatccc gggaacattc cgatcggagc
    14581 tgctgtctct cccaggcgag gccaatcccg catgaaccag aaagtgtaaa gggcctcacg
    14641 taagccgccg cgaacaccgg ctcctcttct ccaatcttgg gactggcacc gtctgaacgg
    14701 tactcgcgtg gaacgcatcg tgtgagacga tccgcacggg acattctgcg tggcggtcac
    14761 tcgtgagacg aggcgcaccg ccttactaag gcgacatcga gagttggcgg ctgcgttcga
    14821 tgctgatcgg accagggcag gcgcgcaccc atccggtgcg gcgcgagggg ttctaatcgg
    14881 ctcgactcca cagcttctac gacagttcgc cgcgcttggc ctgctcgcag ccgttgcccg
    14941 cgctcgagcc catcaccgcc tcaaagtcga tctcgcaccc gcgcgcatgg cgcgcgcgtg
    15001 cgcgtgtttc taggagaatc caccgtggca tataccatat ctagttcgag ctccgtgcac
    15061 gcgtcttggc ctgaagccag cttgattccc gcgatctgga gcgcccaaga caatcgttcg
    15121 caatacattc tggaacgcag cggtcccgcg ccagcgccgg ccgaagtaga gctcgagcac
    15181 gtcaccgcgc tcgaagtcat catgctttgg gggagccaag ttctgcgcgt cgaacacctg
    15241 aatccaccgc gcaacgtgtc tctcggggat cgaccactgg cagccaattc agcggcgacc
    15301 agaggcaccg atttctacat gccgagtgag gcactcggcc gatcttccgc cgagatcgtg
    15361 aaggtcgaag gcgatcaggt gcactgctcg atcttcccgg ggatgcgggc gttcctcgag
    15421 gaacgcggtg cactgcgcga gatcgagctg agccatgacc ttccggtaga cgtggagcag
    15481 cccgaagcgc gacgtttgtc gctgcgttca ggccagcgcg taacgctcaa aattgctgat
    15541 ttctcactgc aaatcgcgcc ggtttcggca ggcaagcccg tagatcgcgg cttgcgcgca
    15601 gcctggaacg gagccgcggc aggctacttc ggattgtctc tcggcgttca cgccgggttg
    15661 ctggcagcgt tcgctttctt cgcgcctcca ctcgggctat ccgatgcaga cgagctcgat
    15721 cgggatcgcc tgtacgtgat gcagagttac ctggatgcgg cagccgagcg cgaacgcctg
    15781 caggaagaaa cgccggaggt tgccgcggcc tccaaggccg acggtggaac cggcagccgc
    15841 tctactggag aagagggctc catgggcaac cccacttcgc gggcaacgca gagacgttac
    15901 gcggtgaagg ggccgcgcga caatccggat ccgacgctgg ctcgagagca agccgtgaaa
    15961 gaggccgccc aatttggcgt gattgggcta ctcagcgcag ggctcgccgg tgatcccaat
    16021 tccccgactg cgatttgggg cgcggatcac gcgtcggggc gggatgacct gagcgcccga
    16081 ggcaatatct ggggggagga aatcggtgaa gcctttggcg ccggtggatt atcacttacg
    16141 ggagttgggg aaggcgcggg gggcccgggc aagggagttg ggttcggaga cgtggggacc
    16201 atcgggcatg gtcggggctt gggagatggt caaggtttcg gaaacagcgt gggcggcccg
    16261 ggctcgcgag gccacgtcgg tcgtgtgccc accatgcatc ccggagtgac gagtgtgtcg
    16321 gggcggctcc cgcccgaggt gattcaacgc gtggtgcggc agaattttgg gcgttttcgg
    16381 atgtgcttca acgaggggct tcggcgcaat ccgaacctcg aagggcgtgt ggcggctcgc
    16441 ttcgtgattg atcgctcggg tgccgtgtct gccgtgagca atggcggctc ggacctgccg
    16501 gatagcgcgg tggtgtcttg tgtcttatcc gcgttttacg gcctcagctt ccctgagcca
    16561 caaaatggca tcgtgtcggt cgtgtatccc atctcgttcg cgccgggttg attcacgcgg
    16621 cgtcttcgtg gcaacttcgc cgcgatgcag atagtgatct tgggcgctgg ctttacgggc
    16681 actgcggtca ccgagctggc actggcccgg ggtcttcgag ttctggccac gacccgcgac
    16741 gcggagcgag cacaccagct tgagcaacga ggcgcccagg tgctgctcgc tcccgccctc
    16801 ccggcatcgg atctgctgcg acaggtctct tccgaaagtc gggttttagt gacgttcccc
    16861 ccggatggtc ataccgatgc ggcccttgcg gaagccctgt cggtgtgcgc gcgcatcgta
    16921 tatatctcgt cgacggcggt atacggcgcg cacaccgggc tcctcgacga gacgacaccg
    16981 gtgcttccca ataccccgcg aggagagcgc cgatttgcgg ccgagcgcgt ctggcacgac
    17041 gcgggagcaa cgatcctacg cgcgcccgcg atttacgggc ctggacgtgg cctacatcta
    17101 cgcctgacgc gaggcgactt gaagctcggc cccaacccga cgaacacgat atctcggatt
    17161 caccgcgacg acttggcgct cgcggcgctg gagatgctcg gccgcaagct cgagcattct
    17221 acctacttga ttggcgatct cgaacccgcg ccgcacctgg aggtcgtgaa ttggttggcg
    17281 accgagctac ggctgcccgc gccagttccc gacgcgagcg tcaccccgga cgaaacgctc
    17341 acgagcgatc ggcaagtcaa cgcgctgcgg ctctggcacg aactcgagct gcggcctcgc
    17401 tatcctacgt atcgtgaagg cctgcgccac tgtatccgtg tcgatctgcc aaactgggtt
    17461 ggcgccgcca gctcggacac ggttcattca gaggggaacg gttaaaccac cgcgtccgcg
    17521 agcgcgctct gcacggctcg accgagttgg tccagcgtat agggcttggg taaccaagct
    17581 gcgaagcgag agttcgattc aatggtgctg ggcagcgtcg cgtgaccact caccatgatc
    17641 gcgcgctgct ccggaaacaa gcgttgcact ttctccagca ctccgagccc gtctacatcc
    17701 tcccccagct ggacatccaa aatcaataag tcacaggggg actcacgctg ttgacggcta
    17761 gccaattcga accgctcaca cgcccttctc ccgctggtct cggtggtcac ctcgtgcccc
    17821 tgatgagaga gcacccgagc cgcggtccgg agctgcagcg cgtcgtcgtc gaccacccag
    17881 attcgcacct tgcgactgcg caccgaaggc ggcggctctt cgcggggccg cagggactct
    17941 cggctacgct cgaagtacaa ggtgaagcgg gtgcccaccc cgggcgtact ctcgacgtcc
    18001 aggaagcctc cgtgctcttt gaccacgccg tgcacgaccg aaagaccgag cccgctaccg
    18061 ctgtgctcac ccgttcgctt cttcgagaag aagggctcga acacgcggcc tagatccgcc
    18121 tgcggaatcc cccgtcccgt atcctccacg cacactacgg catacgagcc cgccgctatg
    18181 gtctcataac ccgccaacgg gccctgcaga acgaccccgc gcgtgctgac gctgacacgt
    18241 cctcgtccgt cgatcgcctc tcgcgcgttg cgcagcaagt tggagatcgc ccgaagcaga
    18301 tgcgcctccg aggctttgac gattagagcg tcaggccaga gtgagtgcac gatctcgacg
    18361 ccgtgtatcg cgtcctcggg ccgccccgct ttgcgaaagg gttgagtgga aatcgatccc
    18421 tccgcttgat gagatctctc ctgttgcacg cacgcaacca cgacccggtt caaatccaaa
    18481 gaatccttgc tcgtgcgtcc ttgccgagcg gaggtgagca ggtctttgat ggtttgggcg
    18541 gcgcgcaacg cggccgtctt gattgcggcg atgtcggcgc gcacctctgg gacctccaaa
    18601 ggcgttgggc tgcgttcgcc gagcgagccc tcgagctccc gcaaaatcac gtccgggagc
    18661 cccaccagcg gccctaagga gttattcaga tcatgagcta cgccgccggc gagcacgttg
    18721 agagcgtgca tgcgacgtgc ggtggccagg cgctcctgag taccccgctc gtgcgcggcc
    18781 gtggtctcga tgatctcacg ctgaagcttc acactcttca gcgcggcact gacctgctcg
    18841 cgcagcatct cgtggggggc tgcacgcgga tctacttgca gcacgaagat gccgaactga
    18901 tcggcttcca cggcgagggg ccaaacgaag tatgtcccgc tgtccgcgtc ttcgcccacg
    18961 aggtcgggga cgagttcggt cgccgcgtaa ggctgctcgc ggaccgcctg ggctttgccc
    19021 ccgcgcaggc acaagaagga tctgagctgc cgcctcgagt cgtccgtgta caacgagagc
    19081 tgcgcgttat gaactcccat cctcggtaat gaggtggcga gaatgtgagc cacttcactc
    19141 agctcgaacg ctgtaagcag gcgctcccca ccacggagta gatgccaata tcccacgtcg
    19201 gcctgcgcgc ggttcctggc ctgagcgtag gtacgctcgc gcgcggtcaa ggcaaacacc
    19261 tcgaaccagc cgtcgtcatg acggtattcc gagggagccg ttctgcgcag ctcggccaaa
    19321 gcagcgtcga gttcgtcata gtcgaccgaa gtctcagcag cttgggccat ccaatcctcg
    19381 agccagtctc gcacgtgaat tggttgctcg gctcggacga gtgcctcggc cgttaccgct
    19441 gccgacgatc ccagcccctt caaacaagag gcgactcttc ggatgcgaac gtccgggtcc
    19501 aagtcatgcc catgtgatct cttggagcgc gtcgccatgt cctcgaagca gccgcaagag
    19561 cgcctggcca cgaaggtcac gggcagaacg cgctgatcgg gaacctgctg cccgaggatg
    19621 ccggcgcgca cggtggcgac ggcacaacgc gccatctcga ccaggggttg tcgcacggtc
    19681 gttagagacg ggttacagat ccgagccaaa ggcgcgtcat cgaagccagt tacccggacg
    19741 ccgcgaggaa ctcgccagcc tgcggccttg agcgcgtcga tggccccgag agcgatccca
    19801 tcattggcac ccactaccgc gtcgaaggct accccgcgcg atagtatctc gcgcatggct
    19861 tcgatgccgc gcggaagggt gaagagacat tccgtttcga gcgcggggtc gtggacgatt
    19921 ccgcgctcgg cgagcacctg caagaagacc tgccgccgca ggctggcgtc gggattgcca
    19981 gagggccctc ccaagtaggc caagcgccga caaccatgaa catcgatcag gtgctccacc
    20041 gccgcgcgca tgccgggagt gttgtccgcc acgacgctcg gaataccggg aatcaccatg
    20101 cccaagctgc agcgcggcaa gcccaccatc ttctccaaca acgcctgaac gccaggcgca
    20161 ccggtgaaag ctgcgagccc gggagaaacc gcgatcacac catctagaca atcggtatga
    20221 gcgagatcgt aaatgctgtt ttgcgctcta tagccgggat cttcaagagg ccgcccgaag
    20281 aagaaaaaca agtcgagccc aaactcctga cacgcggagt gaaatccact gcggagctgc
    20341 gtctcgtaac cggcgctgag atagtcggca tgatcgatca gaacgccgat cgccttgcgc
    20401 tgagatctcg aagggctctg cgtgatcacg accgcttaaa gctagcagtc cgcgggttgc
    20461 agaacagcgc cgcaacctgt cagacacgac agagaccatt gcttacactt tcagacataa
    20521 cagggcagtt cgagaattca gatccgcgcg ctccacgcca acccacacgt agcgcggcaa
    20581 ggcgatgccg agggaatcga cccgttcagc gcccagtact ccgcgagaaa tctcctgcga
    20641 ccacgctcac agctccgcac cgaggcttgc gaaacgctcg gcgaaatccg cactgtcgag
    20701 cgggatctct gaaatcgtca acgctagggt caatgcaacg accgccgaaa cgacatcgag
    20761 aatccccggt ccccagcagg aaagctcggg cggccgtggc ctgcggccgg ttgagcccag
    20821 ccgtcaaaag ccaaggcgtt actcagagcg ggccgccgca cacgcacgaa ctcggaagga
    20881 gcgtcgcggg acggccctgc cgcactgcac gcgggggcgg cggtcaggaa ttgggaagca
    20941 aaggcgccga gcagcttggc ggtgtcgtca ccccgataga cggtgtactg gtcatagtcc
    21001 tcgtagagcc gcggaccatc tctatcagag agcatccaga ccatgctgcc gttgatgcct
    21061 ttgcggagcg ctaactgatt ccagcgttcg taggcgcgta gccggtcgtg caaccctttg
    21121 acgatccgcc cgtggtcgtc ccgaacgact ttcactccgt actcctcgag gatcgtgggt
    21181 ttgccgagct gacgggccag ccgcgcgtga tcggcgatcc acttttctcc ccaactcaga
    21241 cgggcccccc aatcttccgg ataggcgtga aacgtgccga agtctatgtc gggcaaggcg
    21301 gtaagcgccg cgtgatcgac accatcgttg gccttgtagg cccaatgctc gccacccaca
    21361 tccagaaatc cctcatcacc gaccgaaacc atgtgatttg gatctagcga tttgatatgg
    21421 gcggacattt cgtcggccca ggtcacgatc gtttggttgg tccagccgct ggcgccaggg
    21481 cccgaaccct tgcaacgcgg ttcgttcgcg agctcccaag caaagatagc cgggtcgtcc
    21541 cggtacagct tgcccgaata ggtatttttc cgcgcgatca ggtgcgcgac gtagttcttg
    21601 taagcctgct taacaagagg atcggtatag aactcgtggt gcttggtccg cccgaaccag
    21661 gcgagatatt ggtccatgcc gccgaatgct tcccagttgt tcgtcagcac caggattagc
    21721 ttgaggccgc gctcggccgc tttcgccacc gcatagtcta ggcgcctgag cccattctca
    21781 ccgtcattgt acgcggggcg aagagccttg gtgtcccaat actggtagta gactccatcc
    21841 ttcttgccgt ccgcgttcgg atcggtgtgt cggacattgc cgtcgagtga tccgatgtcg
    21901 atgaatgccc acgttcgtac caccgcaaat cccaaagcac gggcagagtc gagtagcgcg
    21961 tcgaccgaag cagaaggctt gtatcccaag taatagttat tggcgcccgc gaaacagaaa
    22021 ggacgacctc cgagctcgaa attcgcgggg ggattcaggc ccgcgtccaa cgtggacgcc
    22081 gcgacatgat cgggagtctt caagcacccc ggcagtgcaa tcaccaacag cgcgctcaac
    22141 gcaagcgtgc ggtgagctgc ctgagcatcg accataacct ccgcgtctta tcagatctgc
    22201 tcggcagctc gcagaacttt ctcgttgttg gcgagagagc acgccgcaaa atcgtgcgcg
    22261 attcggcccc gaatgcgact tcgcgtgctg cgatttcagg tcatgcgatt cgtaagctgg
    22321 cccattgggg ctgaagctgc ggcgagccgc agctcgattc ttcgcttttc gtaggcgtgc
    22381 ctttcgtaca tcccggaggt gagccccaag ctgctggccg cggttacatc gagacggtcg
    22441 agctggatgc gatcacgctc gccgtgatcg tgatcgctgg cgcgagcttg catccatctt
    22501 tcgtacactc gagcccgtgc gggttacgcg cgcccgcgct gacctttcgg gatgacttcc
    22561 gtacttcaag aaaaatgtga ctgggaacga caggctaccc accccgcacg gattgccgag
    22621 cgacagtttc gcaggccaag agtatgccca tccagaaact gcgccgcgcc acccacacat
    22681 gggctaatgt gacgcacttc gggcagcacg cccggagcgc tcttgtctcc gcagcagaag
    22741 aacccatgct cctctttggt cagcgagctc agatgcttcg aagttcagtc ggcgcgtacc
    22801 ctcgagcgga ctcggacgat gccgtcgcgg gcgcacgcgg cctcggaggt tgaaagagcc
    22861 ttgcgccgcg cctcttcgac catcctgctc gcgttggccc ttggcgcctg tgacgctacg
    22921 gtcgagccga ttcacgtcag gagcggctgc ccagcccgtc ccaatcacga ggcgaacacg
    22981 ttggcgcgag cggatatcat cgacgacttc gaagacgaag atttggatct gctcgtcgct
    23041 aacggtcgag atggctcgtg gatcgtaggt tgggacatga cctcggagag gccgctcacc
    23101 gaaggtgcga gcacctgcgc ggcgagcggc cgcgccgcag ggcacttctc ggcatccggt
    23161 ttcacggact ggggagcaaa ctggaccgcc gtgctgcgga gagagtcgaa cgggagggcg
    23221 gtcccgtacg acgcatccga ttactcgggg atctctttct gggcggcggt gggcgcggac
    23281 gcgcccgtgc tcaccaactt acccgttggg gtgacgacca tggacgtggc ctggaatggg
    23341 ggcgtctgct ccggcacttg catggactat tatcgcacca cgatctccgt gacgcgtgat
    23401 tggcagcgct tcgtagtccg cttcgaggcc ttggcgcagc gaggttttgg ggatccgctc
    23461 accgcgctaa aacgtgatca gttggtgggg ttcatcgttt ggcccgatcg cgcgttcgat
    23521 ttgtggctcg atgatgtgcg cttcgagcgc tgatcggtgc cccgccgctc tctttcgagc
    23581 atgggcgagg ttcagcccag agggtcagcg caacgcgacc tcgcacagcc cgaacagcgc
    23641 gaaaaatccg ggcccccagc tacccgaatc attgccagcg aactgcacgc gaacgcgctc
    23701 cgcggtcgcg cccgcggtcc cgcgcacgcc aaggcgcaac caggtcagcg gctccactgc
    23761 cgcgtccgcc ctcaggtagg cggcgccggt gaccacatgt tcatcgcgcg cttgcaacgg
    23821 cacacgcgca tcaccatcga aaccgattcg catcagcgcg agcgcagcgc cgagtgttgc
    23881 gatatagggg tgatttgccg ctcgtattcg ggtgaacacg cccacacccg cgaaggaggc
    23941 gcctacgctg gccgagcctt cgacgctcga gatgttagcg gagcgcagcg gagcactcaa
    24001 atccagaagc agcccccatc gctcactcag tgagtgttgt agtgacaacc cgagctgcag
    24061 acccgctcca gctcctccgg ggctgtacaa cgcgccgagc caggcctgca tcccggattc
    24121 ccgaaagctg cccgcacgag gagcgaccga gtcggaagcc gctggcgcgt catctcgatg
    24181 cttttccggt gggattaccg cggcgttttg ggcctgattc ggagggagtt cggatttggg
    24241 agggtcagcg agttcgtgac tcgcggagtc tctctgggca agcaggctgg ttcggagcag
    24301 ctccgccgtc tggagcgcaa tcagcctctc gttggggccg ttcggatctt catctacgat
    24361 cacctgacgc agcaaagatc gaccggacgt cgcatctgcc atccagacct cgacgccctt
    24421 gcggctcggt aatacgcgga tcgctgcgct cgcgtgctcc gcgcgggcca tatcgtccag
    24481 ggatccgctg gcggctctgc gaaccaccgt aaaccccaag ccggtcaact cggccgtgac
    24541 gcgctcgagc atcgagctgc gattgctctc gaccacgagc acacggctcg atccgctctc
    24601 ctccgacgct gccccagccg tcgtggtcag gctccagacg gcggcgacgc agcacaccaa
    24661 tcgggtaatt ctcggcgtcg cccgccggat ccccgcagct cgcctcactc gctggagatc
    24721 ctgcgcgctt gcgcggcgta aggtccctgc ggaaagcgct tcaggtagcg ctcggcttcg
    24781 agccgcgctc ctgcacgatc accttgccga atcttagctt ccatcaagcg cccagccgca
    24841 tcaccaatca aaggcccacc cggcagctcg tccatgtagg ctttgaacca tcgggctgct
    24901 ctggagtagt cgtgttgttg ctcgaacgca atgcgaccga gcgcgaaggc tgcagtcgct
    24961 gcgtccgtgc tgcccgaaaa gcgtttgcga agcgcgagca aagccgtggt agccagctgc
    25021 gatttggcgg agagccgcgc cgcgtcggcc agctcgagca gctccgagcg tgtggctcgc
    25081 tcgcataccg ccggaaaact ggagctctcc gcgattcgca aggcgtccgc cccacgacct
    25141 gaccgaagcg cctcacgcca gttgggctcg gaattcaggg agctcgtcgg cgcaaccgca
    25201 ggatgctgcg cttcggggct cgtccccacc tgctctagct cgcgaccgcg tacgagcgtc
    25261 gatgcttgac ccggatcgac cgaccatgcg gaagccgccg tccccggcgc gggggtgttc
    25321 aggctgaatt tgtccgaacg ctcggcgggc gcggcttctg ggcaattcac ttctaaccgc
    25381 gcgcccgcat ccactgctct cgagccctgc aatccaggcg gaccgtcgag acaggggccc
    25441 agcacacgca ccgaccccgc ccgagtgcgc aacgagaatt tgctcagtga aggattccac
    25501 gccatcgcga actcagttcc gatgacatgc acttggaacg ggccggcttc gaagttccat
    25561 cgcgtctgct tgccggcgcg atgagtgacg ctcacatcca cttcaccctg ttccagcaaa
    25621 acgcgcgccc cgctcggatc agcggcgagc acacgcattc gcccctcttt ggccaacacc
    25681 accgaacttc cgtccgagaa acgcaccggt acggcctgcc cgttgttggc ttgcacaacg
    25741 tcccccagtt ggcccgtcgt aggatgcgcg ccgatctcga acgaaatagg ccgttcgcgc
    25801 aacagtaccc acccgagaac agctgcaatc gcagcgacgc ccgacgccgc gccaacccac
    25861 cacagcccga gtcgaggaac gctcgccgaa gagccccgcg agttccgggc gagctgcccg
    25921 gtggttactt cccgaagcaa gcgagtgcgc gcgatccgga tctgctccga gggatgcgtt
    25981 tcggcggcca acgcgtcctg aatcttcgac gcgacctcag tcagccctgc tttctgagag
    26041 tccatctcgt ccccctttct agccaagtca ggagcgttgg gcgttcgctc actcgctcga
    26101 cgaacgcctt ctcggctcgc gccaagcgcc gcttgaaggt ggctaatgat accccgcagg
    26161 ccgcagcggc ctcgggcaga gtctgaccat caatgatgcg cagcacgaat gccattcttt
    26221 cgccgacggg catttcatcc aggagtcggt aggtctctct caaagcaaaa cgtgcgtctg
    26281 aacatggcgg ctccaactct tgttcttgta accgttgggg cgaaaatatg ctcaaccagc
    26341 gccgccttgc gcgccttcgg atgtgggcgc gagccgtgaa cacggcgatg gtgttcagcc
    26401 aagctttcag acggagcggg tcgtttattt ccgaaatgtg atcgagcgct cggatgaaga
    26461 cctcttgcaa taggtccggc aaatccgcat ccgggcccaa agcggatcgc agcgtgcgat
    26521 gcacgtgaac ggagtattgg tcgtacagtg cggcgactgc gcctggatga ccggcacgca
    26581 acgcctctag caaggcatgc tcatcgccga tgaatttcag gggtgtcacc atgcgctacg
    26641 actcgaaacg aatgactcgt tgttctgctc tgctggcttg ctgtgctcga tccgctcgct
    26701 gtgctcgatc cgctcgctgc ccgggtgctc gctgtgcgtt tgcgttcgtt ggctatcgct
    26761 cactgttcaa gggcctacgc ggtctgcgag ctcgcccggc tttcccttgg tttgccactg
    26821 ttcaagtgtg atgctcaccc ggttcgtcgt cgcatagtgt tcgccgagtc cgtgaaccgg
    26881 ctcagcgtac actttggtaa gagccgttca tttgaaaatg ccgacgcccg gcgggtggag
    26941 cctcagagtg agatcacgac gcccggcaaa aaccaactgc ccgacacccg acgctatgca
    27001 tcacccgggg acgagattgc cggtaggcct cggacctccg gcgcgttccc cgactggacg
    27061 acgggcccca cacggaagaa gctccatgaa gacgacagcc gctccaaggt gagccgattc
    27121 gtcaaggtta cggacacacg ctcattgggg atcgcacagg ctcgctctcg aggacggctt
    27181 ctggggcgtg cctctgacgg tccaccgggg acttcatgca tcagatcaag ctcggctcat
    27241 cagttccaat cgctcttttt ctggccttaa ccgcgtgctc cagtgagccc aacggaggca
    27301 cgaccaaacc tccgggaagt ggcggccaag cgggcgcccc cacatcagga ggcagcacgg
    27361 caacgtcggg tggcaagagc gcctcgggtg gaagcggtgg ggcgagcgga ggagcaacga
    27421 acggtagcgg tggcgtttcg ccgtcaggag ggagcaccag cggctcagga ggaactaccg
    27481 taggtggctc catgacggcg ggtggttcgg caggaacggc aggcgtggct gctggtggaa
    27541 ctcaggcctc gggtggagcc aatacctcgg gtggaagcag cgctttgggt ggaaccagca
    27601 gcggaggaac cggtgcgatc gccggcacct cgtccagcgg cggaatggca ggctctacgg
    27661 gcatcgcagg agccagcggc acgggcggta gcgctggcgg cggctcgatg cactgggtgg
    27721 gcacttgggc gtgcggaccc cagaccacag aaaccaacaa cctgcctcct tctccgggtc
    27781 tctccggcaa cacgctgcgc cagtacgtgc acgtcacgat tcccggtaag cagctacgca
    27841 tccgcctttc gaacgagttc gggaccagcc cagtcacctt cgagagcgtg cacgtcgcgg
    27901 cttccaaggg cggcggagcg atcgatacag caaccgacaa gacgctcgcg ttcagcggca
    27961 tgccgaacgt cactgtgccc gcgggcaaga cggtgctgtc cgacatgttc gactacaatt
    28021 tgccggccct ctcggacttg gcaatctcga tcaagttcgg aactcagtca ggtgatgtta
    28081 cgggccaccc gggctcgaga acgacttctt acctgcaatc cgggaatgcc gtaagcgaag
    28141 cttccctcgc ctccgcagcc aagacggatc actggtactt catcaccggg gtggacgtca
    28201 tggcgcccga atctgccgct gcgatcgtcg tgctgggcga ctcgatttca gatggccgcg
    28261 gctccacgac gaacggcaac gatcgttggc ccgacgcctt ggccaagcgg ctccaggcca
    28321 acgccgcgac cgcgaacgtc gcggtgctca atcaagggat cggcggaaac accgtactcg
    28381 ccggcggcct ggggcccacc gcaaaggctc gcttcgatcg cgacgtcttg ggtcaaaccg
    28441 gggccaaatg gctgatcatc ttcgagggcg tgaacgacat cggtaacaac agcacgggca
    28501 acgttgccaa cgacttgatc gccgccttcg gccagttcat cgagaaggcc cacgccaaga
    28561 acatgaaggt tttcggcgcg cccattacgc ccttcgcggg ctcgcaatac gatagctccg
    28621 ctcacgagac gacgcgacaa acggtcaatc agtggattcg gacgagcgga aagttcgacg
    28681 ccgtaatcga cctcgacgct gccgtgcgcg accccaacaa gcaaaccgcc ctgcttggct
    28741 cgtacgagag cgccgacaag gatcacttgc accttggccc tgcgggttac aaggcgatgg
    28801 ccgacgccgt agatctcact ctattcaagt gatgttcgac tgaatccctg agcgcaccca
    28861 cgagaagccc cgttcacatc tgaccatgtg caagccgacg gcaaacgatc atcagcatcg
    28921 tttttttcgg ggatgtacga gcgagcgcgg acgtggtggg cgcgcgctct aaacacccgc
    28981 gcccgggccc caagctggga cccacgacgg ccatcagcca gggtatgagc ggccctgagc
    29041 caaggtcgca ttccagaact gagtgggcga gggcgtgaac accccgtccg cgccgaggcc
    29101 tcgtaagcgc cgcatctcct ccacctcctc cacgacccaa acccagattc gctcgttgcg
    29161 ggcgcgagcg cgctggacaa agcggggcgt caacacgcag agcgatcggt gcatctcggg
    29221 aatcatcaac atgcctccgg ttcgcggcgc cattgcatcc agcttcacac gctcgagtaa
    29281 gacccgagac aggagctcac tcgaagtcgc gcagcacagc caaccgggtt gcagcttgcg
    29341 gagctcccgc acgatctgcg gtctttcact agcgatcacc acacgtgagg cggctctctg
    29401 gcgcttcacg aggtcgacga cctccctcac tacgcgaggg tgatccgagt gcacgtcgag
    29461 tacacagcag tgattgggaa aactcgagag cacctcctcc acccggggca cccgcgaacc
    29521 acgctcccga aaggggaact cgcccgctcg ttcgaagccg tacccggcat cgagctccgc
    29581 gagctcggca tgagagatgc gagacacgca aagcggctta cccgtagtgc gaccgagatc
    29641 atcatcgtgt gccgcaacca gtaccccatc cgacgttcgg cgaatgtcca gctccagcac
    29701 gatactgggc gcaatcgagg ccgcacggtc gaaggcttcg agtgtgttct cgggccgttc
    29761 tgccgatccg ccgcggtaag cgacgatgcg gaactcctcg gtcatcagcg gatggtccag
    29821 gaacgccatg cggcctaccc tagccgattg aagagctcgt gccttacgtc gcagggcgca
    29881 aacttgtttg cgcacccgtc gagtaaccag cgcccgagcc gcccccacga acgagcaatc
    29941 cgcagccaac ccaagctcga tgcggcaccg gcgcggcgag tacggctcaa tgcatatcgt
    30001 gccccgacat ctcggaacca acttcgacca tacccatcat gccggcatcc gcgtgatcca
    30061 aaaggtggca gtggtacatc cacatgccgg gccgatcgtc gaagcgaacc ccgattcgca
    30121 acgtaccttt gaccggaaca tccgcggtgt ctttccactc gagcggatga acgggacggt
    30181 agtcttcatc cagcacctgg aagaagtaac cgtgcagatg aaaggggtga gcgaaatcga
    30241 tcgtgggatt tgtgatcgtc caaatctgtg tctcacccaa agccgcttgc aaaggaaccg
    30301 cacgtgacgc cggaatgccg ttgattccaa gctccagggg ctcacccgta ccgctgtttt
    30361 gcgtgagctc catctcgacg ggcgtagctc cctgcaggtc caacgcttcg attgagcgct
    30421 gcgggctggg gagggcttct ggaggttcta caggatcgcc gacgaactcg atgtagaaca
    30481 ggtcttgttc cgggcgagcg aaggtagtgc cgaatccccg gtcatatgga acccagcgca
    30541 cgggcactcg cgttccaggc tccccgacgg gcacaatcag cacatcggac cgctccgccg
    30601 gaaccaccac caccatgtcg ctgtcgacgg gggcctccat gcgcccgccg tccccgccga
    30661 tgcgacggaa acgatgctcc ttgagcgcaa tttggtagta ccgagcgcgc gaagtgttca
    30721 cgatacgcca tcgctgcagc aaccccgacc gcaccttgag ggtgggctga accttgccgt
    30781 tgacgagtag ggtgttgccc tcccgcccga acagggtgcc gagatctccg ccagagtccg
    30841 cgggctcgag tgccccggac tcgctgatgc tggcgtcact caacaccaaa gtgagctcgt
    30901 ccccgagctt cgcggtaacg ttcttatcct cttcctcatc cacgaggaaa ggtgcgtaca
    30961 agccgttgcc cacttgcttc gccgagttga cgtgggggtg ataccaaaac aggcccgcat
    31021 ccggcaccac gaagtcatat gtgaatgact gtccgggttc gatctcgggc tgcgagtgcc
    31081 cgggcgtgcc gtccatctcg ctgggaatgc gcaaaccgtg ccagtgaatc gtagtcggct
    31141 ccggcagctc gttcttgaag tggatgatcg cgcggtcccc tcgacgcacg tgaatgaacg
    31201 gccctggtaa cactcccgag tatgaccaaa aaggcgtgcg tttcccgggc acgacctcaa
    31261 tctcgctcac ccgcgcgtcg agcgtagtct cgaaaacgtg aggatccgag ttcgagtcct
    31321 gtgcactcgg caaacgcaac tctgcgtccc aatccggaac ctgccggttc acggtgaagc
    31381 cgcccatcgc ggcacccgcc cccgcgcctc cgactccgcc ggtcgcacca ctcggttggc
    31441 ccgtgccacc gttcccgcta cctccgtgcg cactgctacc ctgggtagcg ccaccgtggg
    31501 gagccgcacc cgacgctccg cccgcagcgc cgccactgac gccgctatcg acgggtgctc
    31561 cgggagtgcc cccctgcgag gagcaagccg cagcggcaat ggccgcggct atcatggggg
    31621 ccatacgaga tgaaagggtc gagcgaggac tgctgatcga gagcaggcgt gaggcgtccg
    31681 tcatctcggc ggcagcttat caggcgcagc gcccaagtcc ggaaaacacc ccgaaataga
    31741 tccgactcgg ggcacttacc cggatgggat atgacagcga tcactgcacc agctgagctc
    31801 tactctcgat tggcctcttc tagagaatcc cagaaggcgc gcacgattcg tttactgagc
    31861 gcgcgccgct
//
LOCUS       DASTKU010000394         4527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1189130, whole genome shotgun sequence.
ACCESSION   DASTKU010000394 DASTKU010000000
VERSION     DASTKU010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189130"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            604..1182
                     /locus_tag="VFQ61_13640"
     CDS             604..1182
                     /locus_tag="VFQ61_13640"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET9955549.1"
                     /translation="MATTKRSETSPGTETASELISKRIEDLGDWRGEMLAKIRTLIKQ
                     TDPEILEEWKWMGTPVWSHDGIVCTGETYKSVVKMTFAKGAALEDPSRLFNSSLDGNV
                     RRAIDIREGEKINERALKDLFRAAISLNVSSKHARGKKPAEKPAAKKAAVPRAAAGKK
                     PAKAPAAAKKPAAAKKPTAAKKPASPVKRSKR"
     gene            1262..1708
                     /locus_tag="VFQ61_13645"
     CDS             1262..1708
                     /locus_tag="VFQ61_13645"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET9955550.1"
                     /translation="MSNLGPVGSAFSELVVEVFRLNGLALAAGDVLAAPAGLTSARWQ
                     VLGVIDHAPATVASIARTMGLTRQSVRQTTEALVTEGYASSQENPEHQRARLIEITAA
                     GRKALRAVERRHALWAERIGARIGASTLSAALPALRAAREAMEESS"
     gene            1705..2289
                     /locus_tag="VFQ61_13650"
     CDS             1705..2289
                     /locus_tag="VFQ61_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955551.1"
                     /translation="MKPVAIPLLPCRSLDDVLPFYEALGFEVTQRQERPNAYAAMRWR
                     GTDLHLFGVASHEASKNNSMCLLIVDEVEVLHARFAEALRRQLGKVPSQGAPRLTRMK
                     PNQTRFTAVDPAGNSVLFVRRGEPDPHEESRRLSPSLSPLGKAIRAAEVLQTFKNDDE
                     AAIKVLQTALRKAAQEDSAERERALALLEQLRNG"
     gene            2361..2756
                     /locus_tag="VFQ61_13655"
     CDS             2361..2756
                     /locus_tag="VFQ61_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9955552.1"
                     /translation="MVLPATVRAMERVIGIGGIFFKSRDPKALSAWYRDHLGVPLDEH
                     GMVTFPTEGDPGPCTLWQPFPQDTKYFAPSQSTFMLNFRVKDLRAMLAQLKAAGVSVE
                     EKVQEESYGKFGWLMDPEGNRIELWQPIP"
     gene            3033..>4527
                     /locus_tag="VFQ61_13660"
     CDS             3033..>4527
                     /locus_tag="VFQ61_13660"
                     /inference="COORDINATES: protein motif:HMM:NF039722.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6311 domain-containing protein"
                     /protein_id="HET9955553.1"
                     /translation="MAIPLLLGALSFFVLAGGGLLRPTNIRWLQHGDPATYYLGWLFY
                     RNSPWQLPLGLNPRYGLEIGSSIMFTDNIPLFAFVFKAFSRLLPEPFQYFGLWLLGCF
                     MLQAWFAWKLLGILTSKTALRAGGSLLFLLAPPFLWRLQGHYAMLDQWSLLAALYLCL
                     GSRTTARGASWPILALTVSLVHSYTTAMVLGLWLTDVFRRIVWPGRTRADWLQLVLVP
                     GAALFGFWQAGFFATGRGVDAEGFGRYRMNLTSLIDASGWSYLLPDIPERAGDYEGFV
                     FLGLGGILLALVSLPALRGAPAALRKKREYWPLLVALVAFTLFACSNRIGFATHELVI
                     PLPKSLIERANLLRSSGRMFWPVYYALFWVMIRGLIKLYSTRVASALMLTIVLLQAVD
                     TSAGWLPIRRDLQLSGSTWDTPLRSAFWSQASRQYTELRMVPPRNQAPGYAPLAYYAG
                     THGMGTNAIYLARVDLDRLNAATRAADRTIRLAHFDERSLLILDPRYL"
BASE COUNT          880 a         1407 c         1370 g          870 t
ORIGIN      
        1 gtaacaccca aagagcagca agcttcgcca tagcgcacac gagagactct ctttaagcta
       61 gcgctcggag ctcactagag cccgacggat tgtgagacgc ggctgatcga cgcccctcga
      121 ccttcaatcg tggacgcgcg acacgcgacg cgttcgccac gtggcggcgt gccaggcatg
      181 aatctgctta catcgagtct gccgttacga cggctgattc ttcgctgctt atcagctttg
      241 gattcgcaat cgatgaaggt gcggctggac gggactcgca ctcatgacga aactcgccca
      301 ctcgcccact cgcccactcg cccactcgcc gaagcagagc gcatcaaccc caccccggca
      361 gatgcgagga gtcgtgcgcg acacctcgcg agcctttccg cgaggtgcta cccgcggcag
      421 ttttggggtt atgcgcggct ggctgaatag attccgagca atgcggtgat gactccaatt
      481 ccaacaccac taaaacagta tttggaggga aagtttccct tcgggcggag ctcgaacgcg
      541 gagcaaaacg gcgctcgcga attcctgcca gcgtgcgagc gcttttgtgc gaatcattgc
      601 gacgtggcga cgaccaaacg atccgagacg agtccaggaa ccgagaccgc atctgagctg
      661 atcagcaaaa ggatcgaaga tctgggggat tggcgcggtg agatgctcgc gaagatccgc
      721 acgctcatca aacaaaccga tcccgagatc ctcgaagaat ggaagtggat gggcacgccg
      781 gtttggtcac acgatggcat cgtctgcacg ggagagactt ataaaagcgt cgtgaagatg
      841 acgtttgcga agggcgctgc cctcgaagat ccctctcgtc tcttcaactc cagcctcgat
      901 gggaacgtgc gtcgagccat cgacatccga gagggcgaga agatcaacga gcgggcgctc
      961 aaggacctct ttcgggcggc gatatcgctg aacgtctcga gcaaacatgc ccgaggcaag
     1021 aagcccgccg aaaagccagc tgccaagaaa gcagccgttc ctcgagccgc agcaggaaag
     1081 aagccagcaa aggcgccggc cgcagcaaag aagccggccg cagcaaagaa gccgaccgca
     1141 gcaaagaagc cagcctcccc cgtcaaacgc agcaagcgct gaagagcaat gcccgcaccc
     1201 ggcgctgcct ttcgatcgcg caaacattga caggttattg tcaaaatgac agtaccctgt
     1261 catgtccaat ctgggccccg ttggaagcgc tttctctgag ctcgtggtcg aggttttccg
     1321 gctcaatggg ctcgcactgg cggcgggtga cgtgctcgcc gcgccggccg gtctcaccag
     1381 cgcccggtgg caggtcctcg gggtgattga tcacgccccc gccaccgtcg catcgatcgc
     1441 gcgaaccatg ggcctcactc ggcaaagcgt tcgccaaacc accgaggctc tcgtgaccga
     1501 agggtacgca agctcgcagg agaacccgga acatcagcgg gctcggttga tcgagattac
     1561 ggccgcggga cgcaaggcgc tgcgcgccgt cgagcggcgt cacgcgcttt gggcagagcg
     1621 gatcggagcg cgcattggtg catcgacgct ctcggcagcg ttgcccgcgc tgcgcgcagc
     1681 gcgcgaagcc atggaggaga gctcatgaaa cccgtcgcaa ttccgttgtt gccgtgccgt
     1741 tcactggatg acgtactgcc attttacgaa gcgctcggtt tcgaggtaac gcaacgccaa
     1801 gagcggccga acgcctacgc tgccatgcgc tggcgaggca cggatttgca cctcttcggt
     1861 gtagcgagcc atgaagcgtc caagaacaac tcgatgtgtt tgctgatcgt ggacgaggtg
     1921 gaggtgcttc acgctcggtt cgccgaagcg cttcgacgcc agctcggcaa ggttccgagc
     1981 cagggtgccc ctcgactcac tcgaatgaag cccaatcaga cgcgctttac cgccgtcgat
     2041 cccgcaggga actcggtctt attcgtccgg cgcggcgagc ccgatcccca cgaggaaagc
     2101 cgcaggctga gcccgtcgct ctcgccgctc ggtaaagcga tccgcgccgc cgaggtgttg
     2161 cagacgttca aaaacgacga cgaagccgcg atcaaagtac tccagaccgc cttgcgcaag
     2221 gccgcgcagg aggattccgc cgaacgagag cgagcgctcg cccttttgga gcagctccga
     2281 aacggttagc ttaactgcgc actccaattt cgtcccaacg attctcgggt gcacccgagc
     2341 acgagaactg gatgcccacg atggttctac ctgctaccgt gcgcgccatg gagcgagtga
     2401 tcggaattgg aggcatcttc ttcaaatcac gtgatcccaa ggccctgagc gcttggtatc
     2461 gagaccacct cggtgtaccg ctggacgagc acgggatggt caccttcccg accgaaggtg
     2521 accccgggcc ctgcaccctc tggcagcctt tcccccaaga cacgaagtac ttcgctccca
     2581 gtcagagcac gttcatgctc aacttccgag tgaaagatct gcgcgcgatg ctcgcccaac
     2641 tgaaggctgc gggggtctca gttgaagaga aggtccagga agaaagctac ggcaaatttg
     2701 gctggctcat ggatcccgag ggcaaccgaa tcgagctgtg gcaacccatc ccgtagctca
     2761 tgagccggtg caacggcaga gccgacaggc cagtcagccg ttgtgacttg agggccgtga
     2821 tttgagttgt ttcaggtgag gagacagtgt ttaaggcagc ccgtgttgct cagaaatagc
     2881 aacgggctct cgcagcgcgc cgtgagcgct ggtgccagga cacaagactc gggcctagtc
     2941 cccgaatgta gctgagggta gtagtgcagg agcaagcgag cgatcctgac gcgccccgat
     3001 cgtcgccggc caagtgggag cgcgcacgaa acgtggcaat acccctgttg ctgggggctt
     3061 tgagcttctt cgttttggcc ggcgggggtc tgctccggcc aaccaatatt cgctggttgc
     3121 agcacggaga ccctgccact tactatctcg gctggctttt ctatagaaac agcccctggc
     3181 aactgccact cggcctcaat cctcgctacg ggctcgagat tggcagttcg atcatgttca
     3241 cggacaatat cccgttgttc gcgttcgtgt tcaaagcgtt ctcgcgattg ctgccggagc
     3301 cattccagta tttcggtctg tggctactcg gctgcttcat gcttcaggct tggtttgcgt
     3361 ggaagctgct gggaatactg acgtcgaaga ccgcgctacg cgcgggcggc agcctcctgt
     3421 tcctgctggc tccaccgttc ttgtggcggc tgcagggcca ttacgcgatg ttggaccagt
     3481 ggtcgttatt ggccgctttg tatctgtgct tggggagcag aacgacggcg cggggagcct
     3541 cgtggccaat tctcgctctt acggtctcac tcgttcactc ctacaccacg gccatggtgc
     3601 taggcctgtg gctgacggac gtttttcggc gcatcgtgtg gccaggacgg acgcgcgcgg
     3661 actggctaca gctcgtcctc gtgcccggcg cggccctgtt cggcttctgg caagccggct
     3721 tcttcgccac cggacgcggc gtggacgcgg agggcttcgg caggtatcgc atgaacctga
     3781 cgtcgctcat cgatgcgagc ggctggtcgt acttgcttcc ggacatcccc gagcgagcgg
     3841 gtgactacga gggcttcgtg ttcctcgggc taggaggcat cctgctcgcg ctggtcagcc
     3901 tgcccgctct acgcggggcg ccggcagcat tgcgcaagaa gcgtgagtat tggccgctgc
     3961 tagtcgctct cgtcgcattc acgttgttcg cgtgttcgaa tcgcatcggc ttcgcgacgc
     4021 acgagctcgt cattccacta cccaaaagct tgatcgagcg cgccaacttg ttgcgttcct
     4081 cggggcgcat gttctggccc gtttactacg cgctcttctg ggtcatgatc cgagggctga
     4141 tcaagctcta ctccactcgg gtggccagcg cgctcatgct taccatcgtg ttgctccaag
     4201 ccgtggacac gagcgccggc tggttaccga ttcgccgtga cctgcagctg agcggcagca
     4261 cctgggatac tccgcttcgc tctgcgtttt ggtcgcaggc atcgcgccag tacaccgagc
     4321 tgcggatggt gcccccccgc aaccaagcgc ccggctacgc ccccctcgcc tactatgcgg
     4381 ggacgcacgg catgggaacg aatgcgattt atctcgctcg ggtcgatctg gacagactca
     4441 acgccgcgac gcgagccgcc gatcgcacca tcaggttggc tcacttcgac gaacgtagtc
     4501 tcctcatcct cgatcctcgt tacttgc
//
LOCUS       DASTKU010000395         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1635582, whole genome shotgun sequence.
ACCESSION   DASTKU010000395 DASTKU010000000
VERSION     DASTKU010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635582"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFQ61_13665"
     CDS             <1..186
                     /locus_tag="VFQ61_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955554.1"
                     /translation="GPRAPSTVGRGDCVHQSPGSAADLRVAEPAQVCRAGPGVAYLVY
                     WGKLCHRRAKSFCGPLG"
     gene            317..1486
                     /locus_tag="VFQ61_13670"
     CDS             317..1486
                     /locus_tag="VFQ61_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955555.1"
                     /translation="MTNARAWITLWTALAGGGLLATGCGSDELTGPSAGESGGQSGGN
                     PATGGTGGASGGRTSGGANTQGGSTTGGASTAGTTSTGGKATNLGKDCVNDTNCGTNS
                     GLSCVTNSAIPQGICTAPCDTDSDCEVFSKGAKCDPGRFCFEGCEIGEQPVPKCHNRV
                     EFGCRPVSELETSTRCQTEAQCSNGVCDEDSGTCVTVVSACLPTCSNDDDCSGGYCNL
                     GTGECVDEKPKGDPIGKACSPDDDQCEGFCVGSGEDAFCSGYCQWGSPLACGWDGMGP
                     ADAACLFPPLFNSDPAGADAAFCGQLCDCDSDCRSPNMKCLSFAAYDLPELIDFWQRQ
                     GLCAQPSAEDAGRTLPTCDGQGGAGGGGNAGSGGEGGGAGNAGEPGSSGAGGSRG"
     gene            complement(1378..>2148)
                     /locus_tag="VFQ61_13675"
     CDS             complement(1378..>2148)
                     /locus_tag="VFQ61_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955556.1"
                     /translation="SARGSGLASAASVLAETRNYGYWPFRLCFEQGLRRAPKLQGRTL
                     FRASLFPTGRMARPVLVHTQLTDRDAVRCLSEAVRHLIYRHRPRRRLDFELSIQLWPG
                     DVPLPPLGVPPSYTRAQAKTVREWLRGRVTGLRTCCAAAVQRDRLVWGRIALQLKLAA
                     DASGERKITEVRELSRFPDPRAPACLREQLELGKLLPQELPEQFEFAVRCGAPPLPVL
                     PSDQPREPPAPDDPGSPALPAPPPSPPEPALPPPPAPP"
BASE COUNT          392 a          638 c          765 g          353 t
ORIGIN      
        1 gggccccggg cgccgagcac ggtggggcgc ggtgattgtg tccaccagtc gccgggtagc
       61 gcggcagatc tgcgcgtggc tgaacccgcg caggtctgcc gcgccgggcc gggcgtggct
      121 tacctcgtgt actggggaaa attatgccat cggcgcgcaa aatcgttttg cggcccgctc
      181 gggtgaggca accggtccgc ctgaacgtcc ggaagatcgc caaggtagtg acttgctggc
      241 ccttccgcgc aatacctttt gcgtgtcgca agccgctcaa tctttccccc ggcggctggt
      301 gagcgaggag agcttcatga cgaacgcacg agcttggatc acgttgtgga ctgcattggc
      361 gggcggaggc ctgctggcaa cgggctgcgg aagtgacgaa ctgacgggtc cgtcggcagg
      421 agagagcggg ggtcaaagcg ggggcaaccc ggccacgggc ggaacgggtg gagcgagcgg
      481 cggacgaacg agtggtgggg cgaacacgca aggcgggtcg actacggggg gcgcgtctac
      541 agcgggcacg acgagcactg gcggcaaggc gacgaatctt ggaaaagatt gcgtcaacga
      601 caccaattgc ggcaccaact cgggtctcag ttgcgtcacc aactcggcga tcccgcaagg
      661 catatgcacc gccccttgcg acaccgactc cgattgcgag gtgttctcca aaggagccaa
      721 atgcgatcca ggacgtttct gcttcgaggg ttgcgagatt ggcgaacagc cggtgcccaa
      781 gtgccataac cgcgtggaat tcggctgccg cccggtgagc gagctcgaaa cgagtacccg
      841 ctgtcaaacc gaagcgcagt gctcgaacgg cgtgtgcgac gaagattcgg gcacctgcgt
      901 gacggtggtg agcgcgtgct tgccgacctg ctcgaacgac gatgattgct ccgggggcta
      961 ctgcaacctg ggtacgggcg agtgcgtgga cgaaaagccc aagggcgacc caatcggcaa
     1021 ggcttgctca cccgacgacg atcagtgcga ggggttctgt gtcggctcgg gcgaggatgc
     1081 tttttgctcc ggttactgcc agtggggctc tccactggcg tgcggctggg acggcatggg
     1141 accggcggat gcagcgtgct tgttcccacc tttattcaac tcggatccgg ccggagccga
     1201 cgccgcattc tgcggtcaac tctgcgattg cgactccgat tgccgcagtc caaacatgaa
     1261 gtgcctctcg tttgcggcgt atgacttgcc cgagctgatc gacttctggc aacgccaggg
     1321 gttgtgcgct cagccgagtg ccgaggacgc gggccgtacg ctccccacct gtgacggtca
     1381 gggtggcgcg ggcggcggag gcaacgccgg ctcggggggc gaaggcggcg gagcaggcaa
     1441 cgccggcgag cccggatcat caggagccgg cggctcgcgc ggctgatccg aaggcagcac
     1501 tggaagggga ggtgcgccac aacgcaccgc gaactcgaat tgctcgggaa gttcctgcgg
     1561 caggagcttc ccgagttcga gttgttcgcg caaacacgcg ggcgctcgcg gatcgggaaa
     1621 gcgtgagagc tctcgaacct ccgtgatctt gcgttcgccc gacgcgtctg cggcgagctt
     1681 gagctgtagg gcgatgcgtc cccaaaccag ccgatcgcgc tggactgcgg cggcgcagca
     1741 ggttcgtagc ccggtcactc gaccgcgtag ccattctcga actgtcttgg cctgagctcg
     1801 ggtgtagctc ggaggcacac cgagaggggg cagtgggaca tcgcccggcc agagttgtat
     1861 cgagagctcg aagtccaaac gccgtcgagg gcggtgccgg tagatgaggt ggcggacggc
     1921 ttcgctcaaa cagcgaacgg cgtcgcgatc cgtgagttgc gtgtgcacaa gtacaggacg
     1981 cgccatacgg cccgtgggaa acagactggc gcgaaagaga gtgcgtccct ggagtttcgg
     2041 cgctcggcgc aagccctgct cgaagcacaa tcgaaacggc cagtatccgt agttgcgcgt
     2101 ctcggccagc accgacgccg cgctcgcaag tccagagccc cgggcact
//
LOCUS       DASTKU010000396         4565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1189604, whole genome shotgun sequence.
ACCESSION   DASTKU010000396 DASTKU010000000
VERSION     DASTKU010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189604"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..252)
                     /locus_tag="VFQ61_13680"
     CDS             complement(103..252)
                     /locus_tag="VFQ61_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955557.1"
                     /translation="MEVCFEELLIALADKLWKGVRNPRLEQRCIDAVAAALGQISMGR
                     FHPAR"
     gene            complement(464..1147)
                     /locus_tag="VFQ61_13685"
     CDS             complement(464..1147)
                     /locus_tag="VFQ61_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955558.1"
                     /translation="MLHPCVRLDGLLGELPPWDAYYADQEREFTIFFPAYAESGHYVT
                     VSQPDKFFEDCSSFYSHRHRPTVMDRTDGDARSGGRLALSHVAAACRRYARTEIVTLN
                     LRSQNLRVAGPADPGKGDAHPPGRGFATRLRAALCSGGIDATEPENWRDSGWLIQCTL
                     DFLSVDVVVAWVGVSSDGEWFVQIYPAKRPSPDATRACHAAAQCIHEALSAFGGFRDF
                     RWETDGDPR"
     gene            1677..2447
                     /locus_tag="VFQ61_13690"
     CDS             1677..2447
                     /locus_tag="VFQ61_13690"
                     /inference="COORDINATES: protein motif:HMM:NF025631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4360 domain-containing protein"
                     /protein_id="HET9955559.1"
                     /translation="MMKQTSWLYRGFSTRTLLATAGTAMACGCGAAESVPEASDTDQS
                     AVRKTTPDEAPTNIITVEDDLSPESRVKPTGPIVDAATASITAKLLTVNGSGCPAGSA
                     TLQPIPSANAFTITFQGYSAARGENLAAAETRRNCLFLLEVKAPTGYTYTPTSASLSG
                     DANLLPGSTGQAVVSYYFPGSPGTAVHSHSVAAGAGQWNTAGAFPSESLIYAPCGAAA
                     LLAINTSVAIPGESDSPAQSTLSLDPSSTVQFQLRECQ"
     gene            complement(2491..2931)
                     /locus_tag="VFQ61_13695"
     CDS             complement(2491..2931)
                     /locus_tag="VFQ61_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955560.1"
                     /translation="MAAVDNDSMGERADVEDFELRLHSYPHRVWGTLFVIAADDGEVR
                     WFAQSDEYRMSPTQNLELNPDAPYECRSVPESAVRALRSFLQQVTLPALPSGRTGLGG
                     AMHTLSARDRLTTVRWDWWSALPDAWSELLKVVNYMERPTDDFV"
     gene            complement(3073..3714)
                     /locus_tag="VFQ61_13700"
     CDS             complement(3073..3714)
                     /locus_tag="VFQ61_13700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9955561.1"
                     /translation="MTLTTSLTDWTDIDIAAHEVARGLGILPGHSTVADGKAWFWSDN
                     PLGNELFALLDRLTTLGFLEKRNEPDLQYRVAPQFRARLGIEDLWQRLSACSPVSLKL
                     VVLRCADLARSREFFEALGLVLTLEQHGSGPQHYSAQLGETVLELYPASGPAVPTRIG
                     IEVPDIQATIAAVAALGNYDYVVRFEPEREPSAALLRDPDGNKIHVSSRSHQR"
     gene            complement(3761..4387)
                     /locus_tag="VFQ61_13705"
     CDS             complement(3761..4387)
                     /locus_tag="VFQ61_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955562.1"
                     /translation="MQLPLNGGTLPVVIAALIATRTCGPRRRFRGKKRYFRRVLRDAA
                     SFQLEHADGGWWNLWHYHADWFGWGNKRWRYRREHLRALALVFLRICKDAAEFTGPFQ
                     TWIYLSGRDAGEDATYLHTPNSNHSPFPMVPHGDVDWDCEPLLPLFRDLLPELPLRIG
                     MCRVFDGYAEPPRVTTSFFIYSPGVGVPLEQPADSLKAKTLDVLGGRH"
BASE COUNT          955 a         1382 c         1380 g          848 t
ORIGIN      
        1 cgcaatctcg tcagcaccgc ggatcatcgt cgtgttgcct cgtgcgggtc gaagcttctg
       61 gtaagccggc gctggccgtc tgcggcgatt tgctcgaaat ggctatcgag cgggatgaaa
      121 acgtcccatc gagatttgcc ccagcgcggc ggcgaccgcg tcgatgcagc gctgctccag
      181 acgagggttg cgaacgcctt tccagagctt gtcggcgagc gcgatgagaa gttcctcgaa
      241 acaaacttcc atggcctccc agcgtgcagg gcaatcccta cacgaacgaa gtcagcgcgc
      301 aatggaaccc gaagctcaac cagcttgcgc aggagtagat cgccagcttc ggcaacgagt
      361 tcaacgtgct acagcaggcg catcggagcg ccgaggcgaa tgaggagttc acgcgcagca
      421 aggaacgagt cgggagtcat gaagcgtccg tcaccgcacc cagtcaacgg ggatcgccgt
      481 cggtctccca gcgaaaatcg cggaaaccgc cgaacgcgga gagggcctcg tgaatacatt
      541 gagccgccgc gtgacatgct cgagtggcgt cggggctggg gcgcttggct gggtagattt
      601 gaacgaacca ctcgccatcg ctcgagacac cgacccaggc tacgaccaca tccacggaca
      661 gaaagtcaag ggtgcactgg atgagccacc cagaatctct ccagttctct ggctcggtgg
      721 cgtcgatgcc gcccgagcac aatgcggcgc gaagacgcgt tgcaaagccg cgaccgggcg
      781 gatgcgcatc gccttttcca ggatcggcag gtcccgcaac gcgcaggttt tgagagcgaa
      841 gattcagggt cacgatttcc gtccgagcgt accgccgaca tgcagcagcc acatgggaaa
      901 gtgcgagcct tcctcccgaa cgagcgtctc catcagttcg gtccattacc gttggccgat
      961 gccggtgcga atagaaactg ctgcagtcct cgaagaactt gtcgggttgg ctcacggtca
     1021 cgtagtggcc actctcggcg taggcaggaa aaaagattgt aaattcacgc tcctgatccg
     1081 cgtagtaggc atcccagggc ggcaattcgc ccagcaaccc gtcgagacgc acgcaaggat
     1141 gaagcatcca agaagcgtga gcccgcattt ggctttcaag aggctccccg tttgctcccc
     1201 gcgcctgcgg tgtttccgtt cttctgacgc tctggcgctt gaagctcctg aatccgacat
     1261 gggcaggcca cacacggatt gcgtgcacaa ttcgcgccca gaagcggcaa tcgaagcgtg
     1321 ccgcgggcag gtcttttctg ctgccgcgct ttccatgaga tcgagagtac acgtctttcg
     1381 tcaactgagt tggtgacgaa gggtgggcca gttgtcgccg gtctggcggc ggagcacgag
     1441 cgctccacgc gagccgtgta tgggacctgc gtgcggctgt ctgcccgttg caccggaagt
     1501 tcgggcccgt tcacccgaaa gtgcccgagg ttacacgata ggagggcaac ccaacggcgg
     1561 agcgaatcgc gctgaacgct tcgccagcat cattcccgat gcgctccgat gctccggtct
     1621 agcgtctggt tcggagtctg cgcggaggcc tgtccgggga actcgagggt atgaagatga
     1681 tgaaacaaac gagctggctc tatcgtggct tctccactcg aactctgctg gccaccgcgg
     1741 gaacggcaat ggcttgcggc tgcggcgctg ccgaatcggt gcccgaagcc agcgatactg
     1801 accaaagcgc tgtccgcaag accacgccgg acgaggcccc gacaaatatc atcactgtag
     1861 aagacgactt gagcccagag agtcgtgtaa agccgacggg tccaatcgtc gatgcagcaa
     1921 cggcgtcgat cacggcaaaa ctcttgaccg tgaacgggtc gggttgcccg gcgggtagcg
     1981 cgacgctcca gcctattccc tctgccaacg cgttcacaat aacgttccag ggctactcgg
     2041 ccgcgcgtgg cgagaatctc gcggctgcgg agacgcgacg aaattgcctt ttcctactcg
     2101 aggttaaagc gccgacggga tacacctata ctcccacgtc agcctcgctg agcggcgacg
     2161 ccaatttgct acccggctcg actgggcaag ccgtggtttc ctattacttc cccggatcgc
     2221 caggaaccgc cgttcactcc cactcagtcg ctgcgggcgc cggccagtgg aacaccgccg
     2281 gagcattccc atcagaatct ctcatatacg ctccctgcgg cgccgcggcg cttttggcca
     2341 tcaatacgag cgtcgcgatc cctggcgaga gcgactcgcc tgcacaaagc acactttcac
     2401 tggatccgag cagcaccgtg caatttcagt tgcgagagtg ccaataggga ttcccgccgt
     2461 tcagaacaag aaacgctgag gtatccccgc tcacacaaag tcgtcggtcg ggcgctccat
     2521 gtaattcact accttgagca gttcggacca ggcgtcagga agcgcggacc accaatccca
     2581 gcgaacggtc gtcaacctgt cgcgtgcgct cagggtgtgc atcgcaccac cgagccccgt
     2641 gcggcccgat ggtagcgcgg gcagcgtgac ctgctggaga aagctgcgga gcgcgcgcac
     2701 ggcgctttcg ggcacggaac ggcattcgta tggagcgtcc ggattgagct cgaggttctg
     2761 ggtcggagac atacgatact cgtccgactg cgcaaaccat ctaacttccc cgtcatcagc
     2821 cgcgattacg aatagcgtac cccagacgcg atgaggatat gagtgcaatc tgagctcgaa
     2881 atcctcgacg tcggcccgtt cacccattga gtcattatca actgctgcca tgttagccac
     2941 gcttagtgct tctaggccac ttctgacgtg attactagag cgcggcagcc agacctagcc
     3001 tgagcggggc ttcgaaaccc aaacgaaccg aagagaattc tttccgcgcg cggcattaga
     3061 aaggaattcg gactaccgtt ggtgacttct cgatgaaacg tggatcttgt tcccatcggg
     3121 gtcgcgaagg agcgccgcgg atggctcccg ctccggctcg aaacgcacga cgtagtcgta
     3181 gttgccgagt gcggcgacgg cggcgattgt cgcttgaata tccgggacct cgattccgat
     3241 gcgggttggc acagcaggcc ccgaggctgg atagagttcg agaactgtct cgccaagttg
     3301 ggccgaatag tgctgtggtc ccgacccgtg ctgctccaac gtaaggacta ggcccaaagc
     3361 ttcgaagaac tctcgtgatc gagcgaggtc ggcgcaccgg agtacgacga gcttcagcga
     3421 caccggggag cacgcggaga gccgttgcca gaggtcctcg attccgaggc gcgcgcgaaa
     3481 ttgaggagcg acccggtatt gtaaatccgg ttcattgcgc ttctccagaa agccgagggt
     3541 ggtcaatcga tcgagcaacg cgaacaactc gttaccgagc ggattgtctg accagaacca
     3601 cgctttaccg tcggcgacgg tcgagtgccc tggcaagata ccaagaccgc gagcaacttc
     3661 atgggccgcg atgtcgatgt ctgtccagtc cgtgagcgac gtcgtcaaag tcatgtgttg
     3721 ctcggctaac tgcgccgcga tagattgtcc acgattcgga tcaatgtcgg cctcccagca
     3781 cgtccagggt tttggccttc agcgagtccg ccggttgctc taatggaacg ccaaccccgg
     3841 gcgagtagat gaagaacgac gtggtcacgc gaggcggctc cgcataacca tcgaagacac
     3901 ggcacatccc gattcgcaac ggtagctcgg gaaggagatc tctgaagagc ggcagaagcg
     3961 gctcgcaatc ccaatctaca tcgccgtgag ggaccatcgg gaacggcgag tgattgctgt
     4021 tcggtgtgtg caaataggtg gcgtcctcgc ccgcgtcgcg accagacaga tatatccagg
     4081 tttggaatgg cccggtgaac tcggccgcgt ccttgcagat tcgaaggaac actagggcca
     4141 atgcgcggag atgttctcgc cggtagcgcc agcgcttgtt tccccaaccg aaccagtccg
     4201 cgtgatagtg ccagaggttc caccaccccc cgtccgcgtg ttcgagttga aaggaggccg
     4261 catcccgcag cactcggcga aagtatcgct tctttccgcg gaaccggcgt cgcggtccgc
     4321 aagttcgagt ggctatcaat gcggcaatca cgactggcaa tgtaccgccg ttcaacggta
     4381 gttgcatcag aaacgaggta gcctgcccgc ttcaacgtcg agtgaggttg cgctccgcgc
     4441 cgtcttgcac cgagaagctg tagtggcgcc cgcaaaaatc atctacgtga acgtgctcgt
     4501 catcgagcgc gaacgcaccg gtgaatatgt cagcaccgcc ggtttgccat tggagtgaac
     4561 cttgc
//
LOCUS       DASTKU010000397         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1635745, whole genome shotgun sequence.
ACCESSION   DASTKU010000397 DASTKU010000000
VERSION     DASTKU010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635745"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFQ61_13710"
     CDS             <1..444
                     /locus_tag="VFQ61_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955563.1"
                     /translation="NRRPPQPRPDFIITGPGFVGSSFVGSRQSILLDAKYRDLWQNEL
                     PREMLYQLAIYAMSQPRGATAAILYPTEDSAAREALIDIRDPFDDSVRAHVALRPIVI
                     PSLLELIRSGDTRERDWARLGLFLVQGASGARWETPASQTFTTSI"
     gene            complement(498..2156)
                     /locus_tag="VFQ61_13715"
     CDS             complement(498..2156)
                     /locus_tag="VFQ61_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955564.1"
                     /translation="MRRAWGAGVGALLAACAPDSGWLDENPLEPNRFSAIDDRSVRVV
                     GGVFAELHDAGSHLLARRPDASADSLFVIHKAKGTLCELAGVVRYQYLPSPSLGRDPI
                     QLPEYIAFIAKGPKGEELRFGTLDCDVVLTLPNADLPLDLTPDGHSIVLANGQLLRIH
                     PVWSQTSVLSSQVTQVHPPAGTDAQWIVQTPDELSFFTRDWESSGRWDGEVTGIFATG
                     RTLYVSDAQGLTQLRTSDSGVELTRVLETDACMLAGFAWTGDAPWLAYYSPCADKRLV
                     ARTTQSNVSNPTYTLPVANVAPDQVRVVSRVSALQPSLRAYGLFDVDPESGLGTLRAI
                     HADGATPRVAGGAALDQLWFDSGTLYVVADLDQGRGRMLAIGADDSAREIASGVPRTD
                     DTSLGPRWFLGNYDGIAADLMRACPDCAAGDTEVIASRVPLTGYVQHSRADFDHALLS
                     DFDGTSGTLRLHAHPELLTEPGVRPAQFAFLEQLPLPGIAYIANPGAEFGSGQLRYTN
                     YELRASGLVGNHVSEFLIVLWPKPALIYTVQAGPEAGLWLARPK"
     gene            complement(2185..2544)
                     /locus_tag="VFQ61_13720"
     CDS             complement(2185..2544)
                     /locus_tag="VFQ61_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET9955565.1"
                     /translation="MRGALAHVTATQQHDSSVRQYLRYGPILYDKLRREVRAGNTRVD
                     LRPAELEILAYLLSNAHRQVTARELMQEVLHTAGDGAAARNQVYELRRKLRQAGVAEV
                     IRTEGRQGYRLCWKTPY"
BASE COUNT          567 a          841 c          890 g          531 t
ORIGIN      
        1 aaccgtcgcc cgccgcaacc acgtcctgac ttcatcatta cgggcccagg ctttgtggga
       61 tcgagtttcg tgggctcgcg tcagagtatc cttctcgacg cgaaatatcg agatctctgg
      121 caaaatgaac tcccacgcga aatgttatat cagctcgcta tttacgccat gagtcaacca
      181 cgaggtgcga cggctgcgat cctctatcca acagaggata gcgctgccag ggaggcgctc
      241 atcgacatcc gtgatccctt cgacgactcg gttcgggcgc acgttgcgct tcggcctatc
      301 gtcattccga gtctgctgga gctcatccga agcggtgaca cgcgggaacg ggactgggcg
      361 cgactgggcc tcttcctggt tcagggggca agcggcgctc gatgggagac gccagcgtct
      421 caaacgttca ccacctcgat atagcggcaa agtaaactga gcaaaacctc gacgggcggg
      481 ccctcttcag tttgaattca tttgggtcgt gcaagccaga gcccggcttc tgggcccgct
      541 tgcaccgtgt atatgagcgc gggcttgggc cagagcacaa tcaagaattc cgagacatgg
      601 tttccgacca agccggaagc tcgcagttca taattggtat agcgtagttg cccacttccg
      661 aattcggcgc cggggttcgc aatgtaggcg atgccgggca gtggaagctg ttcgaggaag
      721 gcgaattgag cgggacgtac gcccggctct gtcaatagtt cgggatgcgc atgcaatcgg
      781 agcgtgccgg aggtgccgtc gaagtccgaa agcagcgcgt gatcgaagtc cgcacgcgag
      841 tgctggacgt aaccggtcag tgggacccga gacgcgatga cttctgtgtc gcctgcggcg
      901 cagtcggggc aggcgcgcat caggtcggct gcgattccgt cgtagttccc tagaaaccac
      961 ctcgggccga ggctcgtgtc gtcggtgcga ggcacgccgg atgcgatctc ccgcgcgctg
     1021 tcgtccgcgc cgatagctag cattctgcca cggccctgat ccagatccgc gacgacgtac
     1081 agagttccac tgtcgaacca gagttgatcg agcgccgccc cgccagccac tcttggggtg
     1141 gcgccatcag catgaatggc gcgcaaggta cccagaccac tctccgggtc gacgtcgaaa
     1201 agcccgtacg cgcgcagcga gggctggagt gccgagacgc gactcacgac gcgcacttga
     1261 tccggggcga cgttcgcaac gggtaaggtg tacgtgggat tcgacacgtt ggactgcgtg
     1321 gttcgcgcta ccaagcgttt gtccgcgcag ggcgagtagt aggcgagcca gggggcatcg
     1381 cccgtccagg cgaagcccgc gagcatgcag gcatcggtct cgagcacccg ggtgagctcg
     1441 actccggaat cggaggttcg gagctgagta agaccctgcg cgtcgctcac ataaagtgtt
     1501 ctccccgtcg cgaatatccc cgtaacctct ccatcccagc gcccgctcga ctcccagtcg
     1561 cgggtgaaga acgagagctc gtccggcgtc tgcacgatcc actgcgcatc cgtgcccgcc
     1621 ggcggatgta cctgcgtgac ctgcgatgac aataccgagg tttggctcca tacgggatgg
     1681 atgcgcaaga gctgcccatt ggcaagcacg atactgtgcc cgtcaggcgt aaggtccagc
     1741 ggcaaatcgg cgttcggcaa cgtgaggacg acgtcacaat cgagcgttcc gaaccgcaat
     1801 tcctccccct ttggaccctt ggcgatgaaa gctatgtact ccggaagttg gatagggtcc
     1861 cgacccaagc tcggtgatgg aaggtattgg tagcggacta cgcccgctag ctcacacagg
     1921 gtgccctttg ccttgtggat cacgaagagg ctatcggcag acgcgtcggg ccggcgggcg
     1981 agcaaatgtg accccgcatc gtggagctcc gcgaacacac cccccacgac gcgcaccgat
     2041 cggtcatcga tagcgctgaa gcgattgggc tcgagagggt tctcgtcgag ccaaccagaa
     2101 tccggagcgc acgcggctag cagcgcacca actcccgcac cccaagcacg tctcacgttc
     2161 tgagcctttc taaagctgcg caattcaata gggagttttc caacacagcc ggtaaccttg
     2221 gcggccctcc gtgcggatga cctccgccac gccagcttgg cggagtttac gccgaagctc
     2281 gtatacttga ttgcgcgccg cggcgccatc gcccgcggtg tgaaggacct cttgcatgag
     2341 ctcgcgtgcg gtaacttggc ggtgcgcatt agacaatagg taggccagta tttcgagctc
     2401 ggccgggcgt agatcgacac gagtattgcc ggcccggact tcgcgccgca gtttgtcgta
     2461 gagaatcggg ccatagcgaa gatactgccg aaccgaactg tcgtgttgct gagtggccgt
     2521 tacgtgagcg agcgcgccgc gcatccgcca aaggagctcc caagtcccaa agggtctccg
     2581 aagatagtca aaacatactg ggaaaacctc gagttgcgga attggcgtga gcggggttcg
     2641 cggggccacg atgaatgtaa agcaattgcg ccgacttgcg attaccaggt agtcctgcaa
     2701 actagtgcac cgcatgtgag atgcgatcaa gcattcggcg gtggctgcgg atgatagcgg
     2761 cagcgggctt ggtccagatg aagggcgtcg ggtccttgtt ccacccggcg atgtaggaag
     2821 cgatgtggt
//
LOCUS       DASTKU010000398         5856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1860093, whole genome shotgun sequence.
ACCESSION   DASTKU010000398 DASTKU010000000
VERSION     DASTKU010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860093"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VFQ61_13725"
     CDS             <1..820
                     /locus_tag="VFQ61_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955566.1"
                     /translation="ANPRPTKTGGGFAFGSVTKDLRGTGSIRDLGGLLLSVNEADNLS
                     RFALATTQKLCFYANSSACDETDPEFRRVAQAFQARDYSFPVLIKELFASPLVTSAAP
                     TKTSEALGVTVSIARRDQFCQALSGRLGKPDICALSVPLPASAQKNILNIAGNIPADA
                     FGRGSESPVTPTDPTLFFRAASEMLCEDVATQVVEPSAGSGVFSTSDVPGSIAKMVEL
                     VMGYPPADPKNAEAAAILKAHYDAAVAQKANATNALRSTFVAACQSPTSVSFGL"
     gene            881..2344
                     /locus_tag="VFQ61_13730"
     CDS             881..2344
                     /locus_tag="VFQ61_13730"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HET9955567.1"
                     /translation="MLNRRDALLATLFGAGYVGVQAIATGLPAWFIANPKRATAQSLQ
                     CATDAVQNMQYLVVSASSNGDPINCNCPGTYEDATAVHPNDAQLAPTPVDLGGRTYTT
                     ALPWASKEGAGGLMPDAVLARTNFFHYRTGSVVHGDQAKVMKMMGATNNGEMLISLYA
                     KYLSQCLGTVQAEPISVGAGRNATELLSYAGRPAANVSPSSLKAMLGSSGGGTGGGRP
                     AGGGQFGGNSQAAALKSLRTIRDTTMDQLNQLAKRDSTNVQMQFLDALSRSQAQVRSL
                     QDKLATALGSVNGDDVKSQAYAAAALISANVTPVVTMRIAFGGDNHSDQNLAAEVAQH
                     VTGVQGIADLMTALQSLNLIDKVTFATLNVFGRNLNGIAKTDSRSGRDHYGNHAVCVL
                     IGKNIKPGVTGGVLPGSGGSGGPGGGSGSALGAAPIESETGLGSANGDIKPSETYGAL
                     ARTLGVALGLNAADVDKGLNANAGGKVVRTTVVNGTA"
     gene            complement(2391..2795)
                     /locus_tag="VFQ61_13735"
     CDS             complement(2391..2795)
                     /locus_tag="VFQ61_13735"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HET9955568.1"
                     /translation="MTRASHAKPSAPTPEIDRKQGHRREHARFSVDLDVSLGSEHNFY
                     SGFAENLSVGGVFVATHRLRAVGDSVEICVHLPDGSEVHAVGEVRWVRVFNAHSDTPP
                     GMGLHFLKLEPGSEQAIQKFLREREPIFYDGD"
     gene            complement(3079..4932)
                     /locus_tag="VFQ61_13740"
     CDS             complement(3079..4932)
                     /locus_tag="VFQ61_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955569.1"
                     /translation="MRSLLRLVRSLFIAVNLFARPRGMLGGVVLAACVFSACALSPEA
                     ANLIELREVTPRRLEVGDRMRITGRGFPEGRVAELTLRGELLQSGVEKRTGFVLRETA
                     KLVAPHAVEVAITAALAEKLCGPAPRHTTFRGDLELVFFPETTRGSRVSGRLSGVVLD
                     ISPEALHDNARSLEDDARRYAAFLGVTLVTGPSGLRVDTVEPKSRAFRSGMAAGDQVL
                     ELAGVVVRSATDFVPPPNARATELKLWRGNEALTLRVDSTGFRYSAPETLVWAAALVA
                     ALGFGFAGVGTALGRNLAFFENRVAARIRQLTLRLGSKSKSSALLGLAVQTLPEGIGV
                     HLVVLGAAATTTALVLGKSVVAAELDLVVLPAVTAVALLLTLLFTGAGARRWSLWRGL
                     AAAGTAVMANLPLLALWLAAGMHTRALRAEDALSEQGLLPFQWAAFREPILGLLALAC
                     LVGQVPSLRDREALLQGQSTRRQRLIDLAALAHMLSVLGVVSLLTLGGYGQDLDRVSL
                     SVRALAVIAALCKLFALWALVGGLRWLLSNVDWRGLRKAWITRWLVPAACLVGLRAAT
                     GALARHSTYGVLQQLAGPVCFTVFTAAIVYLGIRIVGRVKEPPQAATQPWL"
     gene            5118..5696
                     /locus_tag="VFQ61_13745"
     CDS             5118..5696
                     /locus_tag="VFQ61_13745"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HET9955570.1"
                     /translation="MSKIVERIEKLRAIHSDLLRIEPFQDIGLVGNPGATLAQIEAAE
                     ARIGMRLPPSYREFLRLHDGFPRFFEGTHLLGTRSLGDRSHDAFARAAFAGAESPAMA
                     STPGLASTMRRRRGFERVIPFGVDPQATTLFAFNPDVSRTGGECEVIAWIHELGVRRP
                     DFASFLDLLIEFAEHERTRAGGGSATSLRASA"
BASE COUNT         1076 a         1863 c         1905 g         1012 t
ORIGIN      
        1 ggccaacccc cgacccacca agaccggcgg tgggttcgcg ttcgggagcg tcaccaagga
       61 tttgcgcgga actggctcga ttcgtgatct cggtgggctg ctgttgtccg tcaatgaggc
      121 cgataacctg tctcgttttg cgctcgcgac tacgcagaag ctctgttttt atgccaactc
      181 gtcggcgtgc gacgagactg atccagagtt tcgtcgagtg gcgcaggcgt ttcaagctcg
      241 agactatagc ttccccgtgc tgatcaagga attgttcgcc tcgcccctcg tcaccagtgc
      301 cgcgccgacg aagacctccg aagctctagg ggtgaccgtg agcatcgcga ggcgagacca
      361 attctgccaa gccctgagcg gacgtttggg taagcccgac atttgcgcgt tgagcgtgcc
      421 tttgccggcc agcgctcaaa agaacattct caacatcgct ggcaacatcc ctgcggatgc
      481 gttcgggcga ggttccgaat cccccgtcac tccgaccgat cccacgctgt tcttccgcgc
      541 ggcgagcgag atgttgtgcg aggacgtggc cacgcaggtg gtagaacctt cggctggttc
      601 cggtgtgttc tcgaccagcg atgtgccggg cagtatcgcc aagatggtgg aactggtgat
      661 gggctatccg cccgccgatc cgaagaacgc ggaggcggcg gccattttga aagcgcacta
      721 cgatgcggcg gtggcgcaga aagccaacgc tacgaacgcg ctgcgctcca cgttcgtggc
      781 ggcctgccag tctcccacgt ccgtctcctt tggcttgtga gttttgggtc cgcagggctc
      841 ctctggacaa tgagtctgtt tttcgaagga agcacccccg atgttgaatc gacgtgatgc
      901 actgctcgcg accctgttcg gcgccggcta tgtcggcgtg caagcaatcg ctaccggatt
      961 gccggcgtgg ttcattgcaa accccaagcg cgcgaccgcc caaagcctac agtgcgcgac
     1021 cgacgccgtg cagaacatgc agtatctcgt ggtgagcgcc tcgagcaacg gcgacccgat
     1081 caactgtaac tgccccggca cctacgaaga tgcgactgcg gttcatccca atgatgcgca
     1141 gctcgctcca accccggtcg acctcggtgg aaggacgtac acgacggcgt tgccctgggc
     1201 atccaaagaa ggcgccgggg gcctgatgcc cgacgcggtg ctcgcgcgca ccaatttttt
     1261 tcactatcgg accggatccg tggtccacgg cgaccaagcc aaagtgatga agatgatggg
     1321 cgccacgaac aacggcgaga tgctgatttc gctgtacgcg aagtacctct ctcagtgttt
     1381 gggaaccgtg caggccgagc cgatctcggt cggcgcggga cgaaatgcga ccgagctttt
     1441 gagttatgcg ggcaggccgg cagccaatgt ctcgccgagc agcctcaaag ccatgctggg
     1501 gagtagcggc ggtggcacgg gcggcggccg ccctgctgga ggtggtcagt tcggcgggaa
     1561 ctcgcaggcg gcggcactga agtcgctgcg tacgattcga gataccacga tggaccagct
     1621 gaatcagctg gccaaacgtg actccaccaa cgtgcagatg cagttcctgg acgctttgtc
     1681 ccgaagccaa gcgcaagtgc ggagcctgca agacaagctg gcgaccgcgc tcggctccgt
     1741 caacggcgat gacgtgaaga gccaggctta cgcggcagcc gctctgatct ccgcgaacgt
     1801 cacgccggtg gtgaccatgc gcatcgcctt cggaggagac aatcactccg atcagaacct
     1861 ggcggccgag gttgctcagc acgtgacggg agtgcagggc atcgcggatt tgatgacggc
     1921 gctgcagagc ctgaatctga tcgacaaggt tacgttcgcg acgctgaatg tgttcgggcg
     1981 caacctgaac gggatcgcca agacggactc acgttcgggc cgcgatcact acgggaacca
     2041 cgcggtctgc gtgctcatcg gcaagaacat caagcccgga gtcacgggcg gagtcttgcc
     2101 gggctcgggt ggcagcggcg gtccgggtgg cgggagcggc agtgctttgg gcgccgctcc
     2161 catcgagtcg gagacggggc tcggtagtgc gaacggtgac atcaaaccga gtgagactta
     2221 cggcgccctg gctcgtacct tgggtgtggc tctgggtttg aacgcggcgg acgtggacaa
     2281 gggactgaac gcaaacgccg gcgggaaggt cgttcgaacc accgtcgtga acgggaccgc
     2341 ctaggcctcg tcccctttga ttcttcggcg gaagaatcgg gccgactggc tcaatcgccg
     2401 tcgtaaaaga tgggttcgcg ttcgcgcagg aacttttgga tggcctgctc tgaaccgggt
     2461 tccaacttca ggaaatggag acccatcccc ggaggcgtgt cactgtgggc gttgaacact
     2521 cgcacccatc tcacttcgcc caccgcatgg acttcggagc cgtccggtag gtgcacgcag
     2581 atttccacgc tatcgcccac cgctcgtagt cgatgggtcg ccacgaatac tcctcctacg
     2641 gagaggttct ccgcgaagcc ggagtagaaa ttgtgttcgc tgccgagact cacgtccaaa
     2701 tcgaccgaaa atcgggcgtg ttctcgccgg tgcccctgct tgcgatcaat ctccggggtg
     2761 ggggcagacg gtttggcgtg gctcgctcgg gtcataatgc accgcaggca cggtaaagcc
     2821 ggtcgcaagg tgtgagctcg gcgccgtgcc gctcctgcgg cagaaaacgg tcattgcagc
     2881 gcgagcttta gcgctggacg cctgcaggct cggagtgttt cagaaactcg cagggattaa
     2941 cgcccgggcc ggtttgctgt cccggtatgc gtctggccga gcggctagct gaggctgaag
     3001 cgttacgtac ccgcctgcgc aagcgtgcac ctcgtgaggc acctcccgtg cgagtgcggt
     3061 gacgcgcttc gaccggcgct acagccacgg ctgcgttgcg gcctgcgggg gctctttgac
     3121 acgtcccacg atgcggattc cgaggtacac gatggcagcg gtgaagacgg tgaagcaaac
     3181 cgggccggca agttgctgca ggaccccgta ggtcgagtgt cgagccaacg cgcccgtggc
     3241 ggcgcgcaat cctaccaagc acgccgccgg cacgagccag cgcgtaatcc aagccttgcg
     3301 caggccgcgc caatccacgt tcgagagcag ccagcgtaga ccccccacga gtgcccaaag
     3361 cgcgaatagc ttgcagagcg cggctatgac cgccagcgcg cgaaccgaga ggctcactcg
     3421 atcgagatcc tggccataac cccctagagt gagcagcgac accactccca aaaccgagag
     3481 catgtgcgcg agcgcagcga gatcgatcaa gcgttgccgg cgcgtgcttt ggccctgcag
     3541 caaggcctct cggtctcgga gcgagggcac ctgcccgacc aaacacgcca aggcgagtag
     3601 ccccaaaatc ggctcgcgaa acgcggccca ttggaagggc aagagtcctt gttcggatag
     3661 cgcatcttcc gcgcggagcg cgcgcgtgtg catgccggcc gcgagccaga gcgcgagcag
     3721 cgggaggttc gccattacgg ccgtgccggc ggccgcgagt ccccgccaca gggaccagcg
     3781 gcgggcgcca gctccggtga acagaagcgt gagcaacagc gcgaccgccg ttacggcggg
     3841 taatacgacc aagtcgagct ctgccgctac cacactcttt ccgagtacga gcgccgtggt
     3901 cgtggcggcc gctcccagca ctaccaggtg cacgccgatc ccctcgggaa gggtctgcac
     3961 ggccagcccg agtagcgcgg atgacttgga cttgcttccc aagcgcagcg tcagctggcg
     4021 aatgcgcgcc gccacgcgat tctcgaagaa ggctaagtta cggcccaacg ccgtacccac
     4081 gcccgcaaac ccgaagccga gtgcagcgac taacgcggcc gcccagacca aagtctccgg
     4141 cgcactgtag cggaagcccg tcgagtcgac gcgtagcgtg agggcttcgt tgccgcgcca
     4201 gagcttgagc tcggtagccc gcgcattggg cggcggcacg aagtcggtcg cgctgcgcac
     4261 gaccacgccc gccagctcca gcacctggtc tcctgcggcc atgcccgagc ggaaggcgcg
     4321 gctcttgggc tcgactgtat ctacgcgcag tcccgacggc ccggtcacca gcgttacccc
     4381 caggaacgcc gcatagcggc gcgcgtcgtc ttccaacgag cgcgcgttgt cgtgcaaggc
     4441 ttccggcgag atgtcgagca cgacaccaga caatcggcca ctcacgcgcg aaccgcgcgt
     4501 ggtttccgga aagaatacga gctccaagtc tccgcgaaag gtcgtgtgtc ggggggcggg
     4561 cccgcagagt ttctcggcga gcgcggctgt gatcgcgacc tctacggcgt gcggcgctac
     4621 gagtttggcc gtctcgcgaa gcacgaatcc ggtgcgcttt tcgacgccac tttgcagcag
     4681 ttcgccgcgc agggtgagct ccgccacgcg cccttcggga aacccgcgtc ccgtgatgcg
     4741 catgcgatcc cccacctcca gccgacgcgg tgtcacttcg cgcagctcaa ttaggttggc
     4801 agcttcgggc gacagcgcgc acgcggaaaa cacgcatgcg gcgagcacca cgccaccgag
     4861 catgcctcga ggccgggcaa acaggttcac ggctatgaac agcgaacgga ccaggcggag
     4921 caacgagcgc acgccgccga cgtatacatg gcgggccttg tccgggcgag aaacgcgctc
     4981 aatcagcgcg ttcggtgggg tccgcacgca ctctcgcagg ccgacttggc cctgcacaga
     5041 ggacggcaga ccaatgctgc agagaactgg gccagcgcgc gcggtctgcg atagggcgtg
     5101 acgacgtcgg aggcaccatg agcaagatcg tcgagcgaat cgagaagctg cgcgccattc
     5161 actcggacct gttgcgcatc gaacctttcc aggacattgg tttggttggc aatccgggcg
     5221 ctacattggc ccagatcgaa gccgcggaag cccggattgg gatgcgtttg cccccgtcct
     5281 atcgcgagtt cttgcgcctg cacgacggct ttccccggtt cttcgaaggc acgcacctgc
     5341 tgggcacgcg ttcgctgggc gatcgctcgc acgacgcgtt tgcgcgggcg gctttcgcgg
     5401 gtgccgaatc cccggccatg gcctcgacgc ccggactcgc ttcgaccatg cggcgccgcc
     5461 gtgggttcga gcgcgtcatc ccgtttggcg tcgacccgca agcgactacg ttatttgcat
     5521 tcaaccccga cgtctcacga acgggcggtg aatgtgaagt gatcgcgtgg attcacgagc
     5581 tcggcgtgcg gcgccccgat tttgcgagct tcctcgatct gttgatcgag ttcgcggagc
     5641 acgaacgcac ccgcgcggga gggggctcgg ctacgagcct gcgcgcaagt gcatgagctc
     5701 ggggggctcg cgaagcgctt gcgcgctgat cgcgatcgca ggctgtttca ccaccgcgcc
     5761 cgcttgggca agggacgagt tccctggcaa agttgcggcg ctgttcggct ccaagtgcgt
     5821 tccgccctgt aatctctgtc acatcagtcc gctggg
//
LOCUS       DASTKU010000399         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_296897, whole genome shotgun sequence.
ACCESSION   DASTKU010000399 DASTKU010000000
VERSION     DASTKU010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296897"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..537
                     /locus_tag="VFQ61_13750"
     CDS             106..537
                     /locus_tag="VFQ61_13750"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HET9955571.1"
                     /translation="MPSRHAAAGEEPISSIVQKRGQFDRIGGKRGVRRLVRAFYRHMV
                     NDPAAAELLQMHSDLASAEARLEMFLVEWLGGPRTYSQLRGNRRLRARHFAFRVGANE
                     RDVWLKCMLAALQETVQDAELRAELLTAFRTKAETIVNREN"
     gene            complement(634..1137)
                     /locus_tag="VFQ61_13755"
     CDS             complement(634..1137)
                     /locus_tag="VFQ61_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955572.1"
                     /translation="MSTPSLRLEDSIDFGALPEPINELLQRGVLAYRLSPEAAEEHFQ
                     RALALAPSALPVYFCLYKIHTYGNRLDAALSAAGAGLAEAARQAGWPADFEAWPTNAL
                     AEPNESARFALYTLKALAFIRLKREEVGEAARIIARLRALDPQGYVGWPVVAELLNAS
                     QRGLASS"
     gene            1355..1723
                     /locus_tag="VFQ61_13760"
     CDS             1355..1723
                     /locus_tag="VFQ61_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9955573.1"
                     /translation="MAALGYHRTDAEQALRLIFTAYQEDRPYTLFDVRDAHTFGHGHI
                     PTAQQLGEHDVGHWVRKLPRTQPVFIYCSRGFSSQTFAKTFADFGFSEVYSIDGGFPA
                     LLEAFRAARQAAENSAAAPV"
     gene            complement(1720..2382)
                     /locus_tag="VFQ61_13765"
     CDS             complement(1720..2382)
                     /locus_tag="VFQ61_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET9955574.1"
                     /translation="MQFKPKLYFSPGAVSLVTHIALEELGWPYELERVAIAEGQHLTD
                     RYRRVHPLGRLPALEIEPGIILTETPALLAYVAELAPELQLLPRERLLRARANEWMSL
                     FASAVHVAFITFFRPQRYTSDEAAAARLSLDGKQRFQEMLAYVESRLPDSGYLLGAQY
                     SLCDAYATVFFIWARHFELPVDELPKYRQLAKQVLGRPAVQRALEQEGLGTSREKNGG
                     ST"
     gene            2457..>2817
                     /locus_tag="VFQ61_13770"
     CDS             2457..>2817
                     /locus_tag="VFQ61_13770"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9955575.1"
                     /translation="MQGTLEVRDFRLVRAIAEAGGATHAGRHLNLSQSAVSHQLRGLE
                     QRVGVALFRRSGRKLAITPAGQRFLEVGRQVLGPLLQLELELRRASQQARPKLRVGTQ
                     CYTAYHWLPQVLSALAAQ"
BASE COUNT          544 a          907 c          848 g          518 t
ORIGIN      
        1 gctgcgcacg aggtttgcaa tcccctgggt cgagcttggc gctccgagcc tcgatccttc
       61 gagtctcgaa gcatctttcg accgagcgga acttaggaga gcatcatgcc gagccggcac
      121 gcagccgcag gtgaagaacc catctcgagt atcgttcaga aacgcggcca attcgaccgc
      181 atcggcggca agcgaggagt caggcgcttg gtgcgcgcct tctatcggca catggtgaac
      241 gatcccgccg ccgccgagct cctgcagatg cactcggacc ttgcttcggc cgaggctcgc
      301 ttggagatgt tcctcgtcga gtggttgggc gggccacgga cttatagcca gctacgcggg
      361 aaccgccggc tccgcgcacg ccatttcgcc tttcgggtgg gagcaaacga gcgcgacgtt
      421 tggctgaaat gcatgctggc cgcattgcag gagacggttc aagacgccga gttgcgtgca
      481 gagctactga ccgctttccg caccaaggcc gagactatcg tcaaccgaga gaattgaaga
      541 ggggttcccg cccgatacag cccgctccaa ggggggcagc ggatcaactc gaggccaagc
      601 ctctctgctc aactccaggc caagcctctc tgctcagctc gaggctaagc ctctctgcga
      661 tgcattcagg agctctgcaa ctacgggcca tcctacgtag ccttgcggat cgagagcgcg
      721 caggcgtgcg atgatccgag ccgcttcgcc gacttcttcc cgcttgagcc gaatgaacgc
      781 gagtgctttc aacgtgtaga gagcaaatct cgcggactca ttcggctcgg cgagcgcatt
      841 tgttggccaa gcttcgaaat cggcgggcca acccgcttgc cgcgccgcct ctgccagtcc
      901 cgcgccggcg gccgacaatg ccgcatccag ccgattgccg tacgtatgaa tcttgtaaag
      961 gcaaaagtaa acgggcaaag cactcggtgc gagcgcgagt gctcgctgga agtgttcttc
     1021 tgcggcctcg ggggatagtc ggtacgcgag cacccctctc tggagcagct cgttgattgg
     1081 ctcgggcaat gccccgaagt cgattgaatc ctccaagcgc agactgggcg tggacatgcg
     1141 ggcacggctc gaagcaaaaa cgagaccgcg gagccctgca gtcccaagcg gcgaatcacg
     1201 gaggctcgcg tctgcaaaca gacaagatgt ccgatcctcg acacgcagcc cgagcacgag
     1261 ccggtcgtct cgctctccat cgatggattt caagcgcttc gttgcgcaaa caccgactcg
     1321 accgagcggc accgttcttg ctccagggtg cgcgatggct gctcttggat atcaccgcac
     1381 ggatgccgaa caagcgctgc gactcatctt cacggcgtac caggaagatc gaccatacac
     1441 cctgttcgac gttcgggatg cccacacctt cggtcatggc catattccga ccgctcagca
     1501 actcggtgaa catgacgtgg ggcactgggt gcgcaagctc ccgcgcaccc agcctgtctt
     1561 catctactgc tcccggggct tctcgagcca gaccttcgcg aagacctttg ccgacttcgg
     1621 tttcagcgaa gtctacagca tcgacggggg cttcccggcg ctcctggagg cattccgagc
     1681 ggcgcggcaa gccgccgaga acagcgccgc ggctcccgtc taagtgctgc cgccgttctt
     1741 ctctcggctt gtgccaagcc cctcctgttc gagggcgcgc tgcacggcgg ggcgccccaa
     1801 aacctgcttg gcgagctgcc ggtatttcgg caactcatcc accggcagct cgaaatgtcg
     1861 cgcccagatg aagaacaccg tcgcgtaggc atcacacaac gaatactgcg ctccgagcag
     1921 gtagccgctg tctggtaacc ggctttcgac gtaagccagc atttcctgaa accgctgttt
     1981 gccgtcgagg ctcagccgcg ccgctgcggc ttcgtcggaa gtgtaacgtt gcggacgaaa
     2041 gaacgtgatg aatgccacgt gcacggcgct cgcgaacaag ctcatccact cgttggcccg
     2101 cgcgcgcagt agccgctccc gaggcagcag ctggagctcg ggggcgagtt cggccacgta
     2161 agccagcagc gcgggcgtct cggtgagtat gattcccggt tcgatctcga gcgcaggtaa
     2221 gcgtccgagc ggatgaacgc ggcggtagcg atccgtcagg tgctgcccct ctgcaatcgc
     2281 gacccgctcg agctcataag gccaaccaag ctcctccagc gcgatgtgcg taaccaggga
     2341 cacggcgccg ggcgagaaat acaacttggg tttgaattgc atgtcccttg gtagccgtcg
     2401 gccaagcatg agtaaagcgc attgatttca tagaaatcat caattctact catggtatgc
     2461 aaggcacact cgaggtccgc gatttccgtc tggtgcgcgc catcgccgaa gccggtggag
     2521 ccacgcacgc cggtcgacat ttgaacctca gccaatccgc cgtgagtcat cagctgcggg
     2581 ggctagagca acgcgtcggc gtcgccctgt tccgacgttc cggacgcaaa ctagcgatca
     2641 cgccggcggg gcagcgcttc ttggaagtgg gtcggcaagt gcttggtcca ctgttgcagc
     2701 tcgagttgga gctgcgtcgc gccagccaac aggctcgacc caagctgcgc gtcggcaccc
     2761 aatgctacac cgcgtatcac tggctccccc aagtgctgtc agcgctcgcc gcccaac
//
LOCUS       DASTKU010000400         3835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1635932, whole genome shotgun sequence.
ACCESSION   DASTKU010000400 DASTKU010000000
VERSION     DASTKU010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635932"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1980)
                     /locus_tag="VFQ61_13775"
     CDS             complement(<1..1980)
                     /locus_tag="VFQ61_13775"
                     /inference="COORDINATES: protein motif:HMM:NF022812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HET9955576.1"
                     /translation="MSRSIRSTMHYFALISLCAACGSQGEPMAQGGTAGDLNDAGKPG
                     VGGSPGAGGKPGHGGDTTVAGQATAGSGGTPFPSSGGAGTGGQATGGTTTLVTETVPA
                     ALVQALADAKAMNGATLQAAYPLSLKSKLAYDPLASAGVDLIQKSALALTNAELEKLG
                     TNGLVISRNRAFPTFSYGYQTIYSQDLPLYVSADSILFAVHRSFDTLLSALESAYLKP
                     ELGRLLDGMRTRLSTSAIEKPLASDLDLYLTVASSLLSNRVMPPVAGADPAEIQAMYD
                     LAKAGSGHRIVTWFGSSRDEDFSQFQPRGHYAGNVDLEAYFRAMMLLGRVDLRIVETR
                     SDGSQEFHREQFDAAVALNQLLGADRARWTGIDGTIGAFIGERDSMTPDELAQMLSKL
                     GASDLATTNALGDSTIIDELATGGWGAQRIASRIIIKNVEGPTLPLDRSFALFGQRYT
                     VDSHVFVNTTFDRVPGRMMPDPLDVTFAALANDAALPLLAPQLENASYAAGLARTRAL
                     VDAHEPEYWSGSLYTSWLSALRTLSPTATELAAQPSVVGTRAFQDRILNTQLASWAEL
                     RHDTILYAKQSYTSGNTCEFPDAYVDPYPALYARLSTFAERFQQIALGLPADNAVSSL
                     RQGIVSWSTNFETVMSYLERMAQNQRTGTPHDQELM"
     gene            complement(2093..3409)
                     /locus_tag="VFQ61_13780"
     CDS             complement(2093..3409)
                     /locus_tag="VFQ61_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955577.1"
                     /translation="MATQLASPPPAAAPAPAAPKTVPLMSKFDTTLYGFVEFDAINDS
                     TQSLNEPAGNAAIAKPGTFAGDHHRTTFGARNSRIGLKIAVPVSDDIKASGVFEVDFL
                     GNQPPTVSENQLFSTPGLRMRHMFVKVETPIIDALFGQTWQLFGWQGAFHPNTVEIQG
                     VPGEVFSRAPQARLSKTIKSDSVAFEIAVAAARSPQRDSGVPDGQAGMKLSFPGWKGV
                     RTAGSTGTAADPLTIGVSGVARRFKVPEFAAAPKGRRGKTGWGISLDALIPVIPASME
                     KRANALTLTGSFVTGTGIADLYSGLTGGVTFPALPNPNMVNPAPTYTSNVDAGLVTYD
                     AQGELHTIDWRSFIVGIQYYLPPSGKVWVSANYSQMKSENATSYVDAAGAAKIYNKSY
                     WADANLFVDATSAVRFGAEYAYFHQTYGDGVESKNKRFQFSAFLLF"
BASE COUNT          694 a         1145 c         1263 g          733 t
ORIGIN      
        1 catgagctcc tgatcgtggg gggtgcccgt gcgctggttc tgggccatgc gctcgaggta
       61 gctcattacc gtctcgaagt tggtggacca acttacgatt ccctggcgaa ggctcgagac
      121 ggcattgtcg gcaggtagcc cgagcgcgat ctgctggaag cgctcggcaa aagtgctcag
      181 tcgcgcgtag agcgcaggat aaggatccac gtaagcgtcg gggaactcgc aggtgttgcc
      241 cgatgtgtac gattgcttgg cgtagaggat ggtgtcgtgc cggagctccg cccaagacgc
      301 gagttgagtg ttcagaattc ggtcctggaa agcgcgcgtg ccgaccacgg acggctgagc
      361 cgcgagctcc gtcgccgtgg gcgaaagcgt gcgcagcgcg gataaccacg acgtgtagag
      421 gctcccgctc caatactccg gttcgtgcgc gtctaccaac gcccgggttc tggcgagccc
      481 cgccgcgtaa ctcgcgttct cgagctgagg agcgaggagg ggaagtgccg cgtcgttggc
      541 gagcgccgcg aaggtgacgt ccagcgggtc gggcatcatg cggcccggaa cgcggtcgaa
      601 cgtcgtattg acgaaaacat gactgtcgac ggtgtagcgc tggccgaaca gagcaaagct
      661 acgatcgagc ggtaacgtcg gaccttcgac gtttttgatg atgatgcggc tcgcgattcg
      721 ctgcgcgccc cagccgccag ttgcgagctc gtcgatgatc gtgctgtctc cgagcgcatt
      781 ggtcgtggcg aggtcactcg cgccgagctt ggagagcatt tgcgcgagct cgtcgggagt
      841 catcgagtca cgctctccaa tgaacgctcc gatggtgccg tcgattccag tccagcgagc
      901 gcggtccgcg ccgagcagct ggttcaacgc cacggcggcg tcgaactgct cgcgatgaaa
      961 ctcttgagag ccgtcactcc gagtctcgac gatgcgcaaa tcgacgcgcc cgagcaacat
     1021 catggctcgg aagtacgctt cgagatctac atttccagcg taatggcctc gcggctggaa
     1081 ctgggagaag tcttcgtcgc gcgagctgcc gaaccacgtc acgattcggt gaccactgcc
     1141 cgccttggcc aagtcgtaca tcgcttggat ctccgcggga tccgcgccgg ctacgggggg
     1201 catcacgcgg ttcgacagca agctcgaggc aaccgttagg taaagatcca gatcgctggc
     1261 gagcggcttc tcaatcgcac tcgtcgacaa tcgggtgcgc atgccgtcga gcagacgccc
     1321 gagctcgggc ttcaggtacg ctgactcgag tgcgctcaac agtgtgtcga acgaacggtg
     1381 aaccgcgaac agaatcgagt ctgcggaaac gtagagcggc agatcctgcg agtagatcgt
     1441 ttggtagccg taggaaaagg tcgggaaggc ccggtttcta gagatgacga gcccgttggt
     1501 gccgagcttc tcgagctccg cgttcgtcaa tgcgagcgca gacttttgaa tcagatccac
     1561 ccccgcagaa gcgagagggt cataggccag cttgctcttc aacgagagcg ggtacgctgc
     1621 ttggagcgtg gcgccgttca tcgccttcgc gtctgcaagt gcctgcacca gcgctgccgg
     1681 caccgtctcg gtcacgagcg tcgtcgttcc gcccgttgct tgtccgcccg tgcctgcgcc
     1741 gccgctcgag ggaaacggtg tccccccaga tcccgcggtc gcctgtcccg ctacggtcgt
     1801 gtccccgcca tgtccgggct tcccgcctgc accgggagag ccgccaacgc ccggcttgcc
     1861 cgcgtcattc agatcacccg ctgtgccacc ttgggccatc ggctcacctt gtgagccgca
     1921 cgccgcgcac agcgaaatga gcgcgaagta gtgcatggtg ctgcgaatgc ttctcgacat
     1981 ggcgaacctc gcggaagttc ttacgcaagc ttcatgccga attgcggcag cacctaggct
     2041 gcgtcgtgca agcgccgcag cgtggacgct tgcacgaccc atctcgactc gatcagaaga
     2101 gcaagaacgc cgagaactga aagcgcttgt tcttcgattc gacgccgtct ccgtaagtct
     2161 gatggaagta ggcatactcc gcgccgaagc gcacggccga cgtggcatcg acgaacaggt
     2221 tcgcgtcggc ccagtacgac ttgttgtaaa tcttcgcggc gcccgctgcg tccacgtacg
     2281 aagtggcgtt ctccgacttc atttgggagt agttggccga cacccagacc ttgccagagg
     2341 gtggaaggta gtactggatt cccacgatga aagagcgcca atcgattgtg tggagctcgc
     2401 cttgcgcatc gtacgtgacg agccctgcat ctacgttcga cgtgtacgtg ggagcgggat
     2461 tgaccatgtt cgggttcggt aacgcgggga aggtgactcc gccggtgagg cccgaataga
     2521 gatccgcgat gccggttccg gtcacgaacg agccggtcaa tgtgagagcg ttcgcgcgct
     2581 tttccatcga ggcggggatg actggaatca acgcgtcgag cgagatcccc cacccggtct
     2641 tgccgcgtcg gcctttgggg gcggctgcga actcgggtac cttgaagcgt cgcgcgacgc
     2701 ccgaaacacc gattgtcagc gggtcagccg ccgtaccagt gctgcccgcg gtgcgcactc
     2761 ccttccatcc ggggaagctc agcttcattc ccgcctgccc gtctggcacg cccgaatctc
     2821 gttgtggcga acgcgccgcc gcgacggcaa tctcgaaagc gacgctgtca ctcttgatgg
     2881 ttttggagag ccgcgcttgg ggtgcgcgag agaacacctc gccggggacg ccctgaattt
     2941 ccaccgtgtt cgggtgaaac gcgccttgcc acccaaagag ctgccaggtc tggccgaaga
     3001 gcgcatcgat gatcggcgtt tcgaccttca cgaacatgtg ccgcattcga agaccgggcg
     3061 tggagaacag ctgattctcg gaaaccgtcg gcggttggtt gccgagaaag tcgacttcga
     3121 agacgccgct cgccttgatg tcgtccgaaa ccggaacggc gattttgagt ccgatgcgcg
     3181 agttgcgcgc tccaaacgtc gtacggtgat gatcgccggc gaacgttccg ggcttcgcga
     3241 tggccgcatt gcctgccggc tcgttcaggc tttgagtcga gtcattgatc gcgtcgaact
     3301 cgacgaagcc gtagagcgtc gtgtcgaact tgctcatcag cgggacggtc ttgggggccg
     3361 cgggtgccgg cgcagccgcg gggggcggcg atgcgagttg cgttgccaca ggagatgcgc
     3421 tggtcgcggc cggtggcggc ggagctggtg cgggtgcggg gaactgcgct gattgctcag
     3481 gtgtgaccgg cttcgatgaa ggcgattcgg cttctacgcc tgcagcgggt ggggccggga
     3541 ctgctgcatc gggttgcggc ggtgccgatt gcgcccatgc agatcgagaa acccagacga
     3601 atccaagtat ggtcagcgat gagaagaatc tgtgcatttg tctttccgaa ggcttcagga
     3661 ggccgcgagg gtacaacttt tcccgaaacg cctgcgcgtc ttctgcgtga tcgctgaccg
     3721 tgatctcgct ctcgcaggcg cgccgattgt gacaacgcgg ttacagcgaa gtcgcagtca
     3781 cgccttgttc gcgagaatta tggcggaaaa gcaacgcgag cgggcgcgtc ggacc
//
LOCUS       DASTKU010000401         9129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1860214, whole genome shotgun sequence.
ACCESSION   DASTKU010000401 DASTKU010000000
VERSION     DASTKU010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860214"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VFQ61_13785"
     CDS             <1..952
                     /locus_tag="VFQ61_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9955578.1"
                     /translation="KDSETLVSAGGVSITVGEVQRRLNSLSEAQFAALGPAQEAPKRF
                     VEQVIVPELLSSLEARQKGLDKAPKFNDRERDVLRQSLDMALRDQLKKEKPVTPEDVK
                     AYYEQNKTRYQQPQRVRLWRILVDDEATARKLIEQAKAAQSTSKWGELARENSVDKAT
                     HLRQGDLGFVHPDGTTESPRLKVDAGLFRAAQAVKDGELVGQPVKEGGKFAVIWRRGS
                     LPERNRTLDQERESIQAVLERMRVEEARKGVIAGLRAQYVKDEHPDLLQQIPEGMFND
                     GNKSRRGGPGALPGPRPGMPPRPPGPGNPQNANPDAPELP"
     gene            complement(1040..1486)
                     /locus_tag="VFQ61_13790"
     CDS             complement(1040..1486)
                     /locus_tag="VFQ61_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955579.1"
                     /translation="MIACRRSALRTGAAALLACAFGVKPAFAGSYLNRAALLLHQARR
                     EGEYLRPRYGDKELARIVHEVARARLQAASGMMVPKEVVQAHPHLLLVLENYERAAEA
                     AINLMPEKFLLALQRAQDEERTFRVVMKQLGWDIPSVHKDDDGPNR"
     gene            complement(1483..2220)
                     /gene="rnc"
                     /locus_tag="VFQ61_13795"
     CDS             complement(1483..2220)
                     /gene="rnc"
                     /locus_tag="VFQ61_13795"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET9955580.1"
                     /translation="MSARQSSPPPASAELAARYGLSPDAPHLAQALTHPSYANERREA
                     LDNQRLEFLGDAVLGFCVSDLIYRRFPNADEGTLTRLRAQLVNAEALAEWARSTGVPD
                     ALRLGRGAGASGLRQSTNVLADAVEALIAACFLDSGLGGARAVCEGIVESRLEAFERV
                     GARDPKSELQERLQARGEPPPVYDLVESGGPAHERWFRVRVRVGEQVVGEGVGRSKRA
                     AERTAAEAALAGALAFLLLPSTPPEEA"
     gene            2383..3045
                     /locus_tag="VFQ61_13800"
     CDS             2383..3045
                     /locus_tag="VFQ61_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955581.1"
                     /translation="MAQLNACPFCRALFRPDEGSVCPDCDVALAPMHTLGLSAEAEQD
                     DLLSRPPQLPEHERLPFAFWGRKRGALVALAVLGLVLFFAPWVELIRPESVVRSGYDL
                     ARGRAGWLWGGAVGYLVLLPLALSRRSIFDMRGVRIAAAALASMTLIETLMMVLLPPQ
                     GHRLVPLELEWRWGLFASGVVSLLATFCGATFGGALPPLPVTPVELKPEVPMRRGKRT
                     LH"
     gene            complement(3102..4178)
                     /locus_tag="VFQ61_13805"
     CDS             complement(3102..4178)
                     /locus_tag="VFQ61_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955582.1"
                     /translation="MKARPAFYAGLVALVAATAHAEPLDPALERLVVDSSCHDASGRW
                     TPGAAGYCTPNNASFKKLVNQLGFAMAPNAFYAARTTGYGGFEVAIEASYTKISSDAE
                     YWRLGTQGKSDPNTRRAATENGSVPGLLSLYSIKLRKGFGFGLELGGQVGFMPGSSLL
                     SGGADVRFAILEGFRRGTLAMFPDVSVGAGVRTITGSSDLQLTVAGLDGRLSKPLPLQ
                     DTLVLTPWLGYQHLWIFGDSGLVDLTPGTNALQSCGYAGNNTPINPDPSKSALDGQPV
                     CTATGPNARGDFNNNAVFQHARLERHRLMIGAHLRYEMLLAGAQLATDLTAPADAQSS
                     TSDQNILKDESRQWTIALELGAAF"
     gene            complement(4245..4883)
                     /locus_tag="VFQ61_13810"
     CDS             complement(4245..4883)
                     /locus_tag="VFQ61_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955583.1"
                     /translation="MSDAAAFGAAISRPNQPGAAISRRRRPNSVACLASLLGGVPFTA
                     IALTLSSTTAHAAPQANTALVPGVCISQGSREDSGLAFCGAVRQDLLFLRGNPRAVGF
                     GPYASIGTLAFDDLRISLGASLLLPTLEDLPLVLSAGALTTNFGRVGVESSVFFGLRS
                     YNFHGTYNLAGGLLLQLQQDLDRDARRSVGLGIQVDAMVLALPVLLLWGATR"
     gene            complement(4923..5693)
                     /locus_tag="VFQ61_13815"
     CDS             complement(4923..5693)
                     /locus_tag="VFQ61_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955584.1"
                     /translation="MSWLRNSISLAGATTLLGLALSSCADNESMLFVQGVLAIDQTDC
                     VATPEANATMYPSGILDLSLTSRYSAALLVASQLTQRGSREQLRTESARLSLEGAEVT
                     LEDSKGPITSISPNPFTSRGTGLANPAAGTEPGYAAMFVDLVPASISPNLPDGQITAK
                     IRVFGTTLGGQEIESKEFRFQIQVCRGCLVDYGSGKGACVVTEDMQDSAVCLAGQDRR
                     VPCAYCSGGNGVCANVCNNCYQAEASGLCPNGKPSTCP"
     gene            complement(5856..7517)
                     /gene="aroB"
                     /locus_tag="VFQ61_13820"
     CDS             complement(5856..7517)
                     /gene="aroB"
                     /locus_tag="VFQ61_13820"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET9955585.1"
                     /translation="MFSDIQASFATVPARGLAPDPQQRQRSRETMRTALLLNGFMGTG
                     KTTVGRSLADRTGRPFIDLDQLVEARAGQSVEAIFRSRGEPAFRALEREALDSVLVGS
                     SARAPVVALGGGALLDRSTRLRALEHATIVTLQASPEEIKRRVGDASSRPLLAGNPEF
                     RIRELLESRAPGYAECHAQLETDGRSPASLAEKVHELWQRRACVVALGERSYVVEFGQ
                     GLIEQRLLAVLGRPSRTLLVTDRTVAALHGARIEKLLANLPFSSFAVSPGEEHKHIGS
                     VEQIWRAALAAGADRKSRFIGFGGGVVTDMAGFAAATYMRGVDWIGVPTTLLAMVDAS
                     VGGKTGVDLASAKNAVGAFHQPRAVLCDVNVLATESPRGFRSGLAEVVKTALIGDPGL
                     FELLETAPAGFSADSDLALEIVERSVRVKASVVSRDPHEHGARALLNLGHTVGHAIEA
                     AAGYTRLTHGEAVSLGLVAALRIGVKLGHTPVDLAQRVELLLAKLGLPTDLSREPLRE
                     AANLIGHDKKRGASKLKFIVAREVGRVEIEELELATIERETLALT"
     gene            7693..8109
                     /locus_tag="VFQ61_13825"
     CDS             7693..8109
                     /locus_tag="VFQ61_13825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HET9955586.1"
                     /translation="MRSPSSRAGSPSLHARGQRLAAQSGSSSNKSGATTPGVRELSTG
                     RTTVSVKVKPRASKSRILGLRDGALEVAVAAPPVDGEANAELLKTLATRLGVPKSQLE
                     ITSGATGRLKRITVSGLDEDELWQRLEIRPSPEPGG"
     gene            8057..>9129
                     /locus_tag="VFQ61_13830"
     CDS             8057..>9129
                     /locus_tag="VFQ61_13830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9955587.1"
                     /translation="MNSGKDWKFDQAPSREGEVSGGSQVLEALPSAWPRIADLSGCAT
                     RRKAAEALVPIVQSIFGAGRVLVYLVADAASRPFFRAAGSAITTSDPAFCDEFELLRY
                     ARQQGLEVLRLSLPREAVGIVLLDEFEDDATWVAPLSLAVSLAFSAVWAREQSSRGAM
                     RDASSSVYTLAYFVDVASRGLEVARRHERRFSVAMILIDPALGAGAEVANHLQLAAAS
                     SDIVARADDNEFYVLFPETDGVGAFFAKQRLIERLPGALPYHVSVGAACFPQDGTDVS
                     RLMQRAKLRADAFARAANRYFGMAELTLPVLVDTLLALGQSRAETKSFAESLRYLDVS
                     VADARALVLQCAREAVRAGAARV"
BASE COUNT         1573 a         2926 c         3025 g         1605 t
ORIGIN      
        1 gaaagacagc gaaactttgg tgagtgctgg cggcgtctca atcacggtcg gggaagtgca
       61 gcggcgtttg aactcgctct cggaggcgca gttcgcggcg ctcggaccgg cacaagaggc
      121 gcccaagcgc ttcgtcgagc aggtgatcgt gccggagctg ctgagctcgc tcgaggcgcg
      181 ccagaaggga ctggataagg cgcccaagtt caacgatcgc gagcgtgacg tgctcaggca
      241 atcactcgat atggcgctgc gggatcaact caagaaagag aagcccgtca cccccgagga
      301 cgtgaaggcc tactacgaac aaaacaagac gcgctatcag caacctcagc gcgttcgtct
      361 ttggcgcatc ttggtggatg acgaagcgac tgcccgtaag ctcatcgagc aggccaaagc
      421 agcccagagt accagcaagt ggggagagct cgctcgcgag aactcggtgg acaaggccac
      481 gcacctacgt cagggagacc tgggcttcgt gcatccagac gggactacgg agagcccacg
      541 cttgaaggtg gatgccggtc tgtttcgggc cgcgcaagcc gtcaaggacg gggagctcgt
      601 tgggcagccg gtcaaggaag gcggcaaatt cgccgtcatc tggcggcgcg gatctttgcc
      661 tgagcgcaac cgcacactcg accaagagcg cgagtcgatt caggccgtgc tggagcgaat
      721 gcgcgtcgaa gaggctcgca aaggggtgat cgccgggctg cgcgctcaat acgtcaagga
      781 cgagcacccg gacttgttgc agcaaatccc cgaagggatg ttcaacgacg gcaacaaatc
      841 gcgacgcggc gggcccggag ccttgccggg gccgcgcccg ggaatgcccc cgcgtccacc
      901 tggaccgggc aacccgcaga acgccaaccc agacgcccca gagctgccgt gagcgcaacc
      961 cgctgaacga gttgcgagct cgtggaagag tcgtggtgtc ttgccgccaa gcggctgagc
     1021 gagttcgctc ctcgctcgat cagcggttcg gaccgtcgtc gtccttgtgg acgctcggaa
     1081 tgtcccagcc gagctgcttc atcaccaccc ggaaggtgcg ctcttcgtcc tgcgctcgct
     1141 gaagcgccaa taggaacttc tcgggcatca agttgatggc ggcttccgcg gcgcgttcgt
     1201 agttctccag caccaacaac agatgcggat gcgcctggac gacctctttc ggcaccatca
     1261 ttcccgaggc cgcctgtagt cgtgcgcgcg cgacctcatg gacgatgcgg gccagctcct
     1321 tgtctccgta gcgcggacgt aagtactcac cttcacggcg agcctggtgc aaaagcagcg
     1381 ccgcgcgatt gagatagctg cctgcgaacg cgggctttac cccaaatgca caggcgagga
     1441 gagcggcggc gcccgttctc aacgcgcttc gcctgcacgc gatcatgctt cctccggggg
     1501 cgtagagggc aacaacagga aagcgagcgc cccggcgagt gcggcctccg ccgccgtgcg
     1561 ctccgcggcg cgcttggagc ggccaacgcc ctcgccgacg acttgctccc cgactcgaac
     1621 tcgcacgcga aaccaacgtt cgtgggcggg cccaccgctc tcgaccagat cgtaaacggg
     1681 tgggggctca cctcgcgctt gcagtcgctc ttggagctcg ctcttcggat cccgtgctcc
     1741 gacccgttcg aaagcttcca aacgtgactc gacgatgcct tcacagacgg cgcgcgcgcc
     1801 ccccaacccg ctgtcgagga aacatgccgc gatcagcgcc tcgacggcgt cagccagcac
     1861 gttcgtgctt tggcgcagcc cactggcgcc tgctccgcga cccaaccgaa gcgcgtccgg
     1921 gacacccgtg ctgcgcgccc attcggcgag cgcttcggcg ttgacgagct gcgctcgcaa
     1981 gcgcgtcaag gtgccctcgt ctgcattcgg gaagcgccgg taaatcagat cgctcacgca
     2041 aaaaccgagt accgcgtcac cgagaaactc cagacgttga ttgtccagcg cctcccgacg
     2101 ttcgttggca tagctcgggt gtgtgagtgc ctgtgcgagg tgaggtgcgt cgggacttaa
     2161 accgtaacgc gcagcgagct ccgcggaggc ggggggaggc gaggattgac gcgccgacat
     2221 gggcgatcac ggagggtagc ccgcccgggg ctccggcgcg atactctaag tgccgtgctc
     2281 gctcggaggg cacccggctc gcggcacgtt agggcgatgg tagcgccggc tcgggcgcat
     2341 ttaacgaacg agcgtctcct ctcacgagga taaggattcc caatggcaca actgaacgct
     2401 tgcccatttt gccgggcgct gtttcgcccc gatgaaggca gcgtctgccc ggattgcgac
     2461 gtcgcgctcg ctccgatgca taccttaggg ttgtcagcgg aggcggagca agatgacttg
     2521 ctctcgcgtc ctccacaatt gcccgaacac gagcgtctgc cattcgcttt ttggggcagg
     2581 aaacgtgggg ccctggtggc attggccgtg ctcggcttgg tgctgttttt cgcgccgtgg
     2641 gtcgagctga ttcgccccga aagcgtggta cggagtggct acgacttggc gcgtggaaga
     2701 gcgggttggc tgtggggcgg agcggtgggt tatctggttc tgcttcccct ggcgctcagt
     2761 cggcgcagca ttttcgatat gcgcggagtg cgcattgcgg ccgccgcgct ggccagtatg
     2821 accctgattg agacgctcat gatggtgctc ctgccacccc aggggcatcg tttggtgcct
     2881 ttggagctcg agtggagatg gggcctgttc gcgagcggag tcgtgagcct gttggcgacc
     2941 ttttgcggag ccacgttcgg tggagccttg cctccgcttc cggtgacgcc ggtggaactc
     3001 aagcccgagg ttcccatgcg ccgtggcaaa cgaactcttc actgaaggag ttgcgcgggt
     3061 caccaaggga tcgtgggctc accccgcccc aggtgggttg ctcagaacgc cgcgcccaac
     3121 tcgagcgcta tcgtccactg ccggctttca tccttcagga tgttctggtc gctcgtggag
     3181 gactgggcat ctgcgggcgc cgttaggtcg gtagcgagct gggcaccagc gagcaacatc
     3241 tcgtagcgca agtgcgcgcc aatcatcagc cgatgtcgct ccaaccgagc atgctgaaac
     3301 accgcgttgt tgttgaaatc gccgcgcgca ttcggaccgg ttgccgtaca cacgggttgc
     3361 ccgtccaaag cggacttgga cggatcggga ttgatcggtg tgttgttacc ggcgtagccg
     3421 caactctgca gagcgttcgt gcccggagtc agatccacga gccccgagtc gccgaagatc
     3481 cagaggtgct ggtaaccgag ccaaggcgtg agcaccaaag tgtcctggag cggcagcggc
     3541 ttcgagagac ggccatccaa gcccgcgacc gtcagttgca gatccgacga accggtgatc
     3601 gtgcggaccc cggcgcccac tgatacgtcg gggaacatcg ccaatgtgcc gcgccgaaag
     3661 ccctccagga tcgcaaatcg cacgtcggct cccccgctca ataggctgct gcctggcatg
     3721 aagccgactt gcccgccgag ctccaggccg aagccgaagc ccttgcgcag tttgatcgag
     3781 tagaggctca ataggccggg tacgctgccg ttctcggttg cggctcgtcg cgtgttgggg
     3841 tcgctcttgc cttgggtgcc gagccgccag tactccgcgt cgctgctgat cttcgtgtaa
     3901 ctcgcctcga tagcgacctc gaatccaccg tagcccgtgg tacgcgcggc gtagaaggcg
     3961 ttcggggcca tggcgaagcc gagttgattg accagcttct tgaacgaagc gttgttcggt
     4021 gtgcaataac ccgcggcgcc cggagtccaa cgtccgctcg cgtcgtgaca gctcgagtcc
     4081 acgaccaggc gctcgagcgc aggatccagg ggctccgcgt gtgccgtggc ggcgacgagc
     4141 gccacgagcc cagcataaaa agcggggcga gctttcattc tcgagggtcc aaaatgcgct
     4201 gagcggaggc tacttgccgg ccgccgggtg cgtcaagccg cccgtcaacg ggtggcgccc
     4261 caaagcagca aaactggcag cgccagcacc atcgcatcga cttggatacc caggccaacg
     4321 gagcgccgtg catcacgatc caggtcttgt tggagttgga gcaacaggcc gccggccaag
     4381 ttgtacgtgc cgtggaagtt gtagctgcgg agtccgaaga acaccgagct ctcgacgccc
     4441 acgcggccaa agttcgtggt cagcgcgcct gccgacagca cgagcggcag gtcttcgagc
     4501 gtgggtagca gtaagctcgc gcccagcgaa atccgcaggt catcgaacgc caacgttccg
     4561 atcgatgcgt acggtccgaa ccctactgcg cgcgggttgc cgcgcaggaa aagcaggtcc
     4621 tgtcggaccg cgccgcagaa cgcaagcccg gagtcttcgc gcgaaccttg cgagatacaa
     4681 acccccggca cgagcgcagt gttggcttgt ggtgccgcgt gcgccgtcgt cgatgacaac
     4741 gtgagcgcga tcgcggtgaa tgggaccccg ccgagtaagc tcgccaagca cgcgactgaa
     4801 ttggggcgcc gccgtcgaga gatcgcggcc ccgggctgat tgggccgcga tattgctgcg
     4861 ccgaacgcgg ctgcatcaga cacggctgcg cctcgatatc ttaacggagt ggggcgacgc
     4921 ggttacgggc aggtgctggg cttgccattg gggcacagcc ccgaagcctc ggcctggtaa
     4981 cagttgttgc agacgttcgc gcagactccg ttgccgcccg agcagtaagc gcaggggacg
     5041 cgccggtcct gcccagccaa gcagacggct gagtcttgca tgtcttcggt gaccacacag
     5101 gctcccttcc cactcccgta gtcgacgaga caaccccggc agacttgtat ttggaagcga
     5161 aactccttgg attcgatctc ttgtccgccg agcgtggtac caaaaacgcg aattttcgcc
     5221 gtaatctgcc catccgggag gttggggctg atgctggccg gcaccaagtc gacgaacatt
     5281 gcggcataac ctggctccgt gcctgccgcc gggttcgcca agcccgtacc ccgcgaggtg
     5341 aacgggttcg gagagatgct cgtaatcggt cctttgctgt cttcgagcgt cacctcggcc
     5401 ccctctagcg agagccgcgc gctttcggtt cgcagttgtt cgcgagaacc acgctgggtg
     5461 agctgactcg cgaccaagag tgctgcggag taccgactcg tgagcgacag atccaagatc
     5521 ccactcgggt acatcgtggc gttggcttca ggcgttgcga cgcagtcggt ctggtcgatc
     5581 gccaaaacgc cttgcacgaa cagcatgctt tcgttgtcgg cgcagctact cagcgcgagg
     5641 ccgagcaggg ttgtcgcgcc agcgagcgag atcgagttgc ggagccagct catcggagtt
     5701 gcagtctacc gcccgtgctt cgcgcatgcc aaaaatgtga gccggcccac ggtcgagccc
     5761 ggggcgcgag ctccgaatgt gcgtcagcgg cttagatcgg caggggtgcc gcccgggcga
     5821 cgagaaccgt cccgggcttg gtaacgagtt acggcttatg taagtgccag cgtctcgcgc
     5881 tcaatcgtgg cgagctcgag ctcctcgatt tccacgcgcc ccacctcgcg cgcgacgatg
     5941 aactttagtt tgctcgcgcc ccgcttcttg tcgtggccga tcaagttcgc cgcttcccgt
     6001 aaaggctcgc ggctcaggtc ggtgggcaac ccgagcttgg ccaacagcaa ctccacgcgc
     6061 tgggccagat cgacgggcgt atggcccaat ttcacgccga tgcggagcgc ggcaaccaac
     6121 ccgaggctga cggcctctcc gtgcgtcagg cgcgtgtaac ccgcggccgc ctcgatcgcg
     6181 tgtccgaccg tatgccccag gttgagcagg gctcgggctc cgtgttcatg tgggtcgcgg
     6241 ctcacgacgc tggccttgac ccgcaccgag cgctccacga tctcgagtgc caggtccgaa
     6301 tctgccgaga agcccgcggg cgcggtctct aggagttcga acaagcccgg gtctccaatc
     6361 aaagcggttt tgacgacctc ggccagcccg ctcctgaagc cacgcgggct ctcggtcgcc
     6421 agcacattca cgtcgcacag tactgcgcgc ggttggtgaa aggcgccgac cgcattcttg
     6481 gcagacgcca gatccacgcc ggtcttgccg cccaccgagg cgtccaccat ggcgagcaag
     6541 gtcgtcggca cgccgatcca atcgacgccg cgcatgtacg tggccgccgc gaagccggcc
     6601 atgtcggtga cgacgccgcc tccgaacccg atgaagcgtg acttccggtc cgcgcccgcc
     6661 gcaagtgccg cgcgccagat ctgttccacg ctcccgatgt gcttgtgctc ctcgccggga
     6721 gagacagcga agctcgagaa cgggagattg gccagcagct tctcgatccg cgcgccgtgc
     6781 agcgcggcca ccgttcgatc ggtgaccagg agcgtgcgtg acgggcgccc cagcaccgcg
     6841 agcaagcgct gctcaatcag gccttgcccg aactccacta cgtaggaccg ctcgccgagt
     6901 gccacgacgc aggcacgccg ctgccacagt tcgtgaactt tctcggccag ggacgctggg
     6961 ctgcgaccat cggtctcgag ctgggcgtgg cactccgcgt acccgggcgc gcgggattcc
     7021 agaagttctc ggatgcgaaa ctcggggttg ccggcgagca gggggcgaga gctcgcgtct
     7081 ccgacgcggc gcttgatctc ctccgggctg gcttgcaggg tcacgatcgt ggcgtgctcc
     7141 agcgcgcgca agcgcgtgga gcgatccaac agcgcgccgc cgcccagggc caccactgga
     7201 gctcgagcgg acgagcctac cagcaccgaa tcgagcgcct cgcgctccaa cgctcgaaac
     7261 gccggctctc cacgggaccg gaagatggct tccacgctct gcccggcgcg tgcttccacc
     7321 aactgatcca agtcgatgaa ggggcgcccg gtgcggtcgg ccaagctgcg gcccaccgtg
     7381 gttttgcccg ttcccatgaa cccattcaaa agcagagccg tgcgcatcgt ttcgcgcgaa
     7441 cgttggcgct gttgtgggtc aggcgccagt ccccgtgccg gcaccgtcgc gaacgaagcc
     7501 tggatgtcgg aaaacatgag ggcggatggg cggtgaggtg ggcacgggta ggcgcccgcc
     7561 gcgatcgtgg ccgctcggcc attcgcgagc cagccccgcg gttttgcgtg gcagttcgtg
     7621 tggggatcgt cgcgcgcgct ccccgctgcc agaagggctt cgcgcggcgt tccgttaggc
     7681 tatgctcgca gcgtgcgctc tccgagctcc cgcgcggggt ccccatcgct acatgctcgc
     7741 ggccagcgtc ttgctgctca gtcgggctcc agttccaaca agtccggtgc caccacgccc
     7801 ggggttcgtg agctgtcgac tggccgcacg acggtgagcg tcaaggtcaa accccgcgcc
     7861 tccaagagtc gtattttggg cttacgggac ggtgcactcg aagtcgccgt agcggctcct
     7921 ccggtggacg gggaggccaa cgcggagctg ttgaagacct tggcgacgcg gctcggtgta
     7981 cccaaaagcc agctggaaat tacttcgggg gccacgggtc ggctcaagcg catcacggtg
     8041 tctggcctcg acgaggatga actctggcaa agactggaaa ttcgaccaag ccccgagccg
     8101 ggagggtgaa gtgagcggag ggagtcaggt gctggaggcg ctgccgagtg cgtggccgcg
     8161 cattgcagac ctttcgggct gcgcgacgcg acgcaaggcg gctgaagcac tggtgcccat
     8221 cgtgcagagc attttcgggg cgggccgggt gctcgtgtat ctggtggcgg acgccgcttc
     8281 gcgcccattc ttccgggctg caggatcggc gatcacgacg agcgacccgg ccttttgcga
     8341 cgaattcgag ctgctccggt acgcgcgtca gcaggggttg gaggtgctgc ggctatcgtt
     8401 gccccgcgaa gctgtcggga tcgtgctgct cgatgagttc gaggacgacg ccacttgggt
     8461 ggcgccgctt tcgctcgccg tatccctcgc cttcagcgcg gtgtgggcgc gtgagcaatc
     8521 cagccgcggc gcgatgcgcg acgcaagctc gagtgtctac accttggcgt acttcgtgga
     8581 cgtggcttcg cgaggtctgg aggtagcgcg ccgacacgag cgccgcttct ccgtggcgat
     8641 gattttgatc gatcctgcgc taggggccgg cgcggaggtc gcaaatcatc tacagctcgc
     8701 ggcggccagc tccgacatcg tcgcgcgtgc ggacgacaac gagttctacg tcctgttccc
     8761 cgagacggac ggggtgggcg cattcttcgc aaaacagcgc ttgatcgagc gcttgcccgg
     8821 agcgctgcct taccacgtga gcgtcggtgc ggcttgcttt cctcaagacg gaaccgacgt
     8881 atcgcgcctc atgcagcgcg cgaagctccg cgccgatgcc ttcgcccgtg cggccaaccg
     8941 ctacttcggc atggccgagc tgacactgcc cgtgctggtg gatacgttgt tggcgctcgg
     9001 ccagtctagg gcggaaacca agtcgttcgc ggaatccttg cgctacttgg acgtgtccgt
     9061 ggcggacgct cgtgccttgg tgctgcaatg cgcgcgggag gcggtgcgtg ctggtgcggc
     9121 gcgcgtgtt
//
LOCUS       DASTKU010000402         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1414136, whole genome shotgun sequence.
ACCESSION   DASTKU010000402 DASTKU010000000
VERSION     DASTKU010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414136"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2501)
                     /locus_tag="VFQ61_13835"
     CDS             complement(<1..2501)
                     /locus_tag="VFQ61_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955588.1"
                     /translation="MRVSYIRSMGRNLNLGRGGVFGATGLIGLALLSACSQAGEVESP
                     QKATASRALVAAPAIGPEIGTDPPVPMQSNIGYNTVAASDGVGFLSVEVAGPSIRGTR
                     VDAQGNVLDLEWLDFGPGSNPSTNIWAISQSYPHVSFGGGHYLVMWSEYAVDDQNNSE
                     SRGIRARLVNPDGTFVGTQNFQVGSGTNMYSPSAVWDGQHFVVTWVGYDSEAQTSGVF
                     VSLLNADGSQVAGSERQATTRGHVTNPRIASRDGHTFLVWEEYGLTPEGFSSGYAIWG
                     TRLSSTGVALDPEGIELSGESTSSTYADVAVGSNDVLVVFQTYDSPYTVRGTLVTHDG
                     QVSKRDFAVSHSTADAANSSVAFDGTDYLVSWADSRGEQYAVYGQKVKPGVGAASDED
                     VKLAAQSPRTVGPISDHTNVVWNGSRYLVTFLGEGVFGSLLEKDLTAVDDSIPLTAIP
                     NKQGYPVSTWNGQNYVVTWTDERESFSDMAVRAVRIDTLGRNLDPAGIAVSPPENPAF
                     SHSLASTGDGSTLFLWSGVSDGPKQRILSSSGTLGAVTSFGDEQLSTVPLIASDGTNY
                     MAVFSTGDSSNGKIFAQAVSGAGVSSPPFDISPSTVNTAPTIFRGESGYWITYSNAGT
                     WLAPLGSAGQVGTPLQVSTQPVWLGTAGGETNALVVWSELDSKVIRARFLKGSAFSGE
                     PFEIATESLGYGATATWDGSSYWLAWETENHHVMGRNLAEDGTLGEISTWIGEGDCAG
                     VTLTSDRQGQLLLSYVRYTNMSNARRVYSRLIGRDAGTIPNGGSGGAGGGTSTNPGGS
                     GTGANAGASAAGASSGGTLSTSAGASSTGGATTASG"
     gene            2863..>3121
                     /locus_tag="VFQ61_13840"
     CDS             2863..>3121
                     /locus_tag="VFQ61_13840"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9955589.1"
                     /translation="MPAPTVRSYLAALAVSLASVSLTSWSASAEPVDYALDMSHSVVG
                     FKVPHLVVSSVSGRFKEVKSSTIKLDDQDLTKSQIAIEIKAD"
BASE COUNT          573 a         1053 c          901 g          594 t
ORIGIN      
        1 ccgctcgcgg tcgtagcgcc gcccgtgctg ctcgcccccg cgctcgtcga tagcgttccg
       61 cctgagctcg cgcccgcggc agaagcgccg gcgtttgcgc ccgtcccgct accgcccgga
      121 ttcgtgctcg tgcctccgcc cgcgccgccg ctacccccgt tgggaatggt cccggcgtct
      181 cgcccgatga gtcgggagta tacgcgccgc gcattgctca tgttcgtgta gcgcacgtaa
      241 ctcaagagca gctgaccttg tcgatcgctc gtcaacgtga cccccgcgca atctccctcg
      301 ccaatccacg tggagatttc tcccaaggtg ccgtcttcag ccaggttacg gcccatcaca
      361 tgatgatttt ccgtctccca ggctagccag taagagctgc catcccaggt tgccgtcgcg
      421 ccgtacccca ggctctccgt ggcgatctcg aagggttcac cgctgaaggc cgagcccttc
      481 aagaagcgcg ctcgaatcac cttggagtcg agctcactcc acaccacgag tgcgttcgtc
      541 tccccgcccg cggtgccgag ccaaaccggc tgagtcgata cctgcagcgg cgttccgact
      601 tgccccgcgc tgccgagcgg agcaagccag gtgcccgcgt tggagtaggt aatccagtat
      661 ccggactcac cacggaagat cgtgggcgcc gtgttgaccg tactcggtga gatatcgaaa
      721 ggtgggctcg acacaccggc cccgctcacc gcctgcgcga agatcttccc gttcgacgaa
      781 tcgccggtcg agaacaccgc catgtagttg gtgccgtcgc tcgcaatcag gggcaccgtc
      841 gagagctgtt catctccgaa ggacgttacg gcgccgagcg tgcccgagct cgagaggatg
      901 cgttgctttg gaccgtcgct cactccgctc cacaagaaca gggtcgagcc atctccggtg
      961 gacgcgaggc tatgactgaa tgcaggattt tcgggcggcg aaacggcaat tccggccggg
     1021 tccaggtttc gccccagggt gtcgatgcgc acggcgcgca cggccatgtc actgaaggac
     1081 tcccgctcgt ccgtccaggt aaccacgtaa ttttgcccat tccaggtcga gactggatag
     1141 ccttgcttat tcggaatagc agtgagtgga atggagtcat cgaccgcggt caggtccttt
     1201 tcgagcagac tgccaaatac accctctccc aggaacgtca ccagataacg cgagccattc
     1261 cacaccacat tggtgtggtc cgagatcggg cctacggtgc gcggcgactg cgcggcgagc
     1321 ttcacgtctt cgtctgaagc cgcgcccact cctggtttca ccttctgacc gtaaacggcg
     1381 tattgctcac cgcgcgagtc cgcccacgag accaagtaat cggtcccgtc gaacgccacc
     1441 gacgaattgg ccgcgtctgc agtcgagtgt gaaaccgcga aatcgcgctt cgaaacctgt
     1501 ccgtcgtgcg ttacgagggt accccgtacc gtgtacggag aatcataggt ttgaaacacc
     1561 accagcacat cattactgcc caccgcgacg tccgcgtacg tcgagctcgt cgattcgcca
     1621 gagagctcga tgccttccgg atctagagca actcccgtgc tggagagccg tgtgccccag
     1681 atggcatatc cggacgagaa cccctccgga gtgagcccgt attcctccca aaccaagaag
     1741 gtgtgcccat cgcggctcgc aatccgcgga ttggtgacgt gcccgcgggt ggtcgcttga
     1801 cgttcggagc ccgcaacctg gctgccgtcg gcgttcagca agctcacgaa tacgccgctc
     1861 gtttgggctt ccgaatcgta gcctacccaa gtgacgacga agtgctgccc atcccaaacg
     1921 gcggatggag agtacatgtt ggtcccgctt cccacctgga aattctgcgt gccgacgaaa
     1981 gtcccgtccg ggttgacgag gcgagcccga atcccgcgac tctccgagtt gttctgatcg
     2041 tcgaccgcgt actcactcca catcaccagg tagtgcccgc cgccgaacga gacgtgcgga
     2101 taggactgac tgatggccca gatattggtg ctcgggttgc tgcccgggcc aaagtcgagc
     2161 cactctagat ccagtacgtt gccttgcgca tccacgcggg tgcctcgaat actcggcccc
     2221 gccacttcga cggacaagaa gcccacgcca tcgctcgccg cgaccgtgtt gtagccgata
     2281 ttcgactgca tcggcacggg tggatcggtg ccgatctcgg gtccgattgc gggcgccgcg
     2341 acgagcgcgc gcgacgcggt tgccttctgc ggactttcga cctcaccggc ctgtgaacaa
     2401 gcgctcagca gcgcgagccc gatcaatccc gtcgcgccga agactcctcc ccgcccgaga
     2461 ttcaagttcc tgcccatact tcgaatataa gataccctca cgactcacct cctgcgccgg
     2521 ctaacttacg cgcgaactcc gcgcgacgct agcgaacatt gagtaacaca ccgagaatga
     2581 tccaaaagcg atccgagccc tgtgccctcg caccgtcacc gagcgcgcgc gaccgggttt
     2641 acccgtagcg ggtgcacccg agcgggcttg aaccgggtgg gcttgaagcg agcgggcttg
     2701 aagcgagcat aggtccgccc gattgttcca gcagcgcgaa aggtccgttc ctgccgcggg
     2761 acttgtcctg ggggcgaaca gcgccatgct ctggacctcg tcgagcttgg ctcgatcgat
     2821 tccatcgagc cttggctcgt tctgaaggag aagtttatcg acatgcccgc tccgaccgtg
     2881 cgttcttatc tcgcagccct cgccgtctcc ctggccagcg tctcgctgac ctcctggtcc
     2941 gcttcggccg aacccgtcga ctacgcgctc gacatgtcgc attcggtggt cggcttcaag
     3001 gttccccacc tggtggtcag cagcgtctcg ggtcgcttca aggaagtgaa gagctccacc
     3061 atcaagctcg acgaccaaga cctgacgaag agccaaatcg cgatcgagat caaggctgat
     3121 t
//
LOCUS       DASTKU010000403        15898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_297452, whole genome shotgun sequence.
ACCESSION   DASTKU010000403 DASTKU010000000
VERSION     DASTKU010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 15898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15898
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297452"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /gene="modC"
                     /locus_tag="VFQ61_13845"
     CDS             complement(<1..1041)
                     /gene="modC"
                     /locus_tag="VFQ61_13845"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HET9955590.1"
                     /translation="MDRVISPPADSRVRVDVTLGSLHLQLALELPGSGICALFGPSGS
                     GKTSLLRLLAGLERWPARVEFGGRVYQDSERGIFVPPHQRPVGYVFQEASLFPHLDVR
                     ANLEYGYRRNPRARLRLPFEQILELLGIGQLLSRSVDRLSGGERKRIAIARALLRGPE
                     LLLLDEPLAELDWARRAEILPYLERLRDELSLPMIYVSHSIEEVARLADHLVLIESGH
                     ALASGPVESVLTRLDVPTAHSDEASAILTGEISAHDPEDQLTRIEIGNEHLWVSRIER
                     PLGTRLRARILARDVSLSRERPAPTSILNLLHARVVEISDDGPSRVNVQLILGDGSTR
                     LLSRITRRSRAAL"
     gene            complement(1013..1690)
                     /gene="modB"
                     /locus_tag="VFQ61_13850"
     CDS             complement(1013..1690)
                     /gene="modB"
                     /locus_tag="VFQ61_13850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HET9955591.1"
                     /translation="MSDDDLTAIRLTVELATLTTSILILLGTPIAYWLATTRSPFRNW
                     VASVIGLPLVLPPSVLGFYLLLGLGPHGFLGRFTTALGLGPLAFTFTGITLGSVVFSM
                     PFVVQPLQNAFESIGTRPIEVAASLGAGPWDRFFSVVVPLARPGFIVAAVSGFAHTVG
                     EFGVVLMIGGNIPGQTRVVSLSLYDHVEALEYAEAHRLAGLLVVFSIVVLFVLNSFRG
                     SRYLSAR"
     gene            complement(1724..2341)
                     /locus_tag="VFQ61_13855"
     CDS             complement(1724..2341)
                     /locus_tag="VFQ61_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955592.1"
                     /translation="MIACAKVEPPGDLRSRFTIEVRVHSGPEPVAGAQLWRGGEHVAT
                     SSESGIARVSLTGEEGERAEIAIHCPAGYGSSTSKLSWTLHALSDAALIPRFDAACTR
                     VERRVVVGLRASNAGELPIRYLNQTVAKTDSSGVAHLELSVLAGQPVTLTLDTTRDAS
                     LLPRNPELTFVASDRSELVLIEQKFSRLVPPAKPLKPAPRVPQRL"
     gene            complement(2446..3456)
                     /locus_tag="VFQ61_13860"
     CDS             complement(2446..3456)
                     /locus_tag="VFQ61_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955593.1"
                     /translation="MNRISEIAFHGRFRMCVHRLLPTGAQLRLALYWACVFVLGAAWA
                     ARCVWAHAQEGVLAVGHELLGLAELTSRAEAIELNGVHVRHASLVTSNSVSATLDRFE
                     SHCLARPGVLAELSQWLTRSEPKRDSGVESHRTWRNGVWREDAGASGFVVCFVGKGAG
                     FGGRGFAVTTVLSQFVANPDLATFGHLRYFYAERVPTGTRAVTLWTDDPVNVRRMFPG
                     DQDASGSDSRWVPRPPNALRLLSAAAEALPLAVRIYDSAERAQVVQDFYERWAQPRGL
                     RRVTRGANSTSYLSENGAQLLVATHEHDARTTVTLSELAAGELLSAAFEAQRSSAEGT
                     RK"
     gene            complement(3527..4096)
                     /locus_tag="VFQ61_13865"
     CDS             complement(3527..4096)
                     /locus_tag="VFQ61_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955594.1"
                     /translation="MESARCSRAQQRGAALVEAMVVISSLMLGVLGLAFFRQVYVSEL
                     SAMRLARAAVLAHAMSGCRTNEPRSWLGRDRGRFEVLGSGQDPEVVSSSAQAASGTSD
                     ANAQRFLARLGSRAEGAGRRLAHPITRAALAGRVHVSSESEGVHRVLSQSIVARSYGS
                     CADPIAEDARIELLSSLKGEFSTFVAGAH"
     gene            complement(4111..5682)
                     /locus_tag="VFQ61_13870"
     CDS             complement(4111..5682)
                     /locus_tag="VFQ61_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955595.1"
                     /translation="MNTANDLAPSDGCRRTSPVFTPRTLDNDERGAILVLGIFMASAM
                     VGILWYVAGIGDALLLRERLQESADAAAFSAAVLHARGMNLIVLLNLLMASILALRVA
                     MKAIQAGLTVAGIVASLLPFAGQPVAALCFEGAAQMQSAVNATREPINRSISALSRLG
                     GVVRELTPAGAIAGSAQLGRRFAPLVSSTVAAGVASLGGLPVRYDEPGRLCRESGESV
                     GQIVTWLLHLPVDRAFFERASALVGKAVAAGGPYFCEMGFGAAPDFSEELEGLPGQAC
                     EEERRGITERRDQARESYLEACARYSAPCAGGSRPPTPAEHAELTELELSLRTAQAAL
                     GHFDAEQCERRKRAQAERAQDELGGLGGDRESSDSQRDQLRPWAIQSRFQNGNAVGQL
                     LALSEGRRDLLSRGLHGVSVAAPAHSKQLESLPPALAFAQAEYFFDCAGPWSNADCDA
                     GDSESMWHLRWRARLRRYSGGEQKLIESFGQLLGLSQGIARSVQGLAERGAPTPGNAE
                     LLRQLSRAAREPVIH"
     gene            complement(5666..6319)
                     /locus_tag="VFQ61_13875"
     CDS             complement(5666..6319)
                     /locus_tag="VFQ61_13875"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HET9955596.1"
                     /translation="MTPFETNCTVEPSLALASGRHPPATANHCALTRCLDDTGAVFVE
                     FLLAFMPVYVFFLCVIQLGLLFAVRLGVEHAAANAARAAAVVIGDDPKRYAGEAAHSL
                     QIGTGRRYRAVFDAALLSLSPWVLNGTLDQLELGLPEESARQGVAARRDRVQLRPMHG
                     ASVDKVRVNVQARAVCLIGLADRIVCSAAKGRSGLPSRTVRAEAVFPYQGANYEYSK"
     gene            6495..6692
                     /locus_tag="VFQ61_13880"
     CDS             6495..6692
                     /locus_tag="VFQ61_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955597.1"
                     /translation="MSKPTVEKRYHHSAILRDERASASVEYLVLVGAVGLSASVGMVA
                     VGVAILRSFDFVRALLLSPIP"
     gene            6763..6969
                     /locus_tag="VFQ61_13885"
     CDS             6763..6969
                     /locus_tag="VFQ61_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955598.1"
                     /translation="MQGESIEMPAQERTTARHRRGATMVEYIIVIGLVALLAIAAFKF
                     FGEKVGNKIKSQGNAVEKINDTAP"
     gene            6998..7546
                     /locus_tag="VFQ61_13890"
     CDS             6998..7546
                     /locus_tag="VFQ61_13890"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET9955599.1"
                     /translation="MLPFLCATLILCFVSTITDLRSARIPNWLTLGGAALGLIGHGAL
                     GWSWGGYPALGEALAGSLSGGLLCGLVPLVLYLKGGLGGGDLKLFVAIGVLCRPLLGI
                     EIQTYGLVTAAVIAPARLAYEGRLLRVALGSLQLATDMFRPKERRRAPPPELLASFRL
                     APAVLVGALLSLIHHSYELLPR"
     gene            7578..8348
                     /gene="cpaB"
                     /locus_tag="VFQ61_13895"
     CDS             7578..8348
                     /gene="cpaB"
                     /locus_tag="VFQ61_13895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HET9955600.1"
                     /translation="MKLSPTWVGLLLATAGVGLQLLYMRRFEADVSGGRKIELLVAAQ
                     PLERGKPVRAEMLGVRAVPQAYVDDRAVRAEDRDKVVNLRATNQVPALQSLAWTDFIA
                     VHDDQRDLSSLVQPGNRAMPIKVQSEESLELIRPGDFVDVLAVDADGREASVLLQRVL
                     VLAAGLETSVERSSEKSTGPSARLLTVSVTLAEAQLLGLAQTIGRLWVVVRGAEDPRV
                     FESPPDVSRSDLFDAHVRQNLQRNARHPVPIRLEPEVP"
     gene            8345..9739
                     /locus_tag="VFQ61_13900"
     CDS             8345..9739
                     /locus_tag="VFQ61_13900"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HET9955601.1"
                     /translation="MNGSPSAFAERPRARPRAAYSKLLSASLWVSAVLLFPSVSPAGS
                     SQRGSSEAVGEVSLSVGETRTLPARDVKNYSEGVQGVIDIKLTSDASQFVLVGRKPGS
                     TTLLLIKNDGSQSSWNIHVFSRAPERVEQELRELLNGLNVRSRRIGSQIVLDGVVAND
                     TELSRVQRITALYPEQVISLVQLATEGVPSPQSGNAKYLIRIDFYFVQYDKNSSYGVG
                     LAWPDSIAAGAVDFSYDFLANTARAATASVSKQPLPSLDLAARRGYAKVLKQAAVVTN
                     NDNEANFSNGGEQNFTVNTGLTVGVQRIPFGTDITVLPHYDPDAKTLSLKLAAEVSDL
                     TASVSGTALPGRSTSRLTTQVSLKLGQSLVLSGIHSHSLTHSVSGLPGLSQIPILGIL
                     FGSHTQAELETEGAIFVVPSVVQSLPNSERDLVDAALKRFETFDGGDLGQLRSFDRTP
                     HSEHGRASRGTHER"
     gene            9729..11369
                     /locus_tag="VFQ61_13905"
     CDS             9729..11369
                     /locus_tag="VFQ61_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9955602.1"
                     /translation="MSGNDEVGSIPPVQHRRLRLSRGEPCTIGRGLENTLVLDSPRVS
                     RHHATLELLGSQLRVADCSRNGSLVGDLYIQHAEVAVDPRTPIQIGEFVLTLEDETAS
                     TAELDFVAVPAQPASAPWPIESPRTSLELRREVHRLLLDHLDLSSTRAARLEDPTLRS
                     RVLSALRRIIHGLEARLDPGIDRDALIGELADEALGLGPLEGLLADPSITEIMVVDPD
                     TIYVEQRGKIVRSRARFTDDDRVRAIIERIVTPLGRRIDESSPIVDARLPDGSRVNAI
                     IRPLALRGSSITIRKFSKAPLALTDLVQFGALTPGMGRFLTRSVAARKNIVIAGGTGS
                     GKTTLLNVLSMAIPADDRIVTIEDAAELKLRQPHVVSLETRPSNMEGKGEYSIRDLVR
                     NALRMRPDRIVVGECRGGEALDMLQAMNTGHDGSLTTTHANSPREALARLETLVLMSG
                     LDLPVRAIREQIASAIHLLVQQSRLSDGSRKITSIAEIAGLGPSGEIEVVPLFEFVRN
                     GTSSSGEVRGEFRATGFLPSFLDQFRVLGLIQPGEPYL"
     gene            11366..12229
                     /locus_tag="VFQ61_13910"
     CDS             11366..12229
                     /locus_tag="VFQ61_13910"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9955603.1"
                     /translation="MRLPYSVETLEYFGLALTFSGLLLGSRAALAPGSGARRAYRAYT
                     AELDRKLAASFLCGSGPRIAALQLALGVGVVTLGLGLAMPYGCVALGPLALAPNWYLD
                     RKRRIHISSIEAQIDGFILGLANALKSVASPAAAIAQLGPALPLPMRLEVDKVNREVR
                     LGSSLDRALLNLSSRLRSSEVDTALSAVLIGLQIGGNLPSVLESTAATLREMKRLDGV
                     IRTKTAEGRAQLWVLALFPFVICLGFSAVDEQYFTPLRHGWLGSGLVCLGLGLWLVSL
                     LLARQILKVEL"
     gene            12235..13134
                     /locus_tag="VFQ61_13915"
     CDS             12235..13134
                     /locus_tag="VFQ61_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9955604.1"
                     /translation="MFSPLSILAVAAWACVFCGCFTLGWVAARAQLPERRRLGLRGVK
                     RAAALAERPAWSAIEPSVRWLGARVVPWLGPATRARLDRQLTLAGDALGLLPEEVTAM
                     SLMCGLAGGSIATVLGWMTGLGPTMTWVGALLGGLIPIWVLQSTGSDRVRYIERRLPH
                     AIDLLALAMGAGLDFPGSMSRVVEKAGTRRDPLVEEFALVLQCLQLGQTRRQALEQFA
                     ARTPSAAVTEFVAALIQAELRGHPVAEVLRIQSEVARRKRSLHAEESAARAGVAMMGP
                     LVLMFLGILVLIVGPMLIRLSGP"
     gene            13185..14048
                     /locus_tag="VFQ61_13920"
     CDS             13185..14048
                     /locus_tag="VFQ61_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955605.1"
                     /translation="MTTLKFDIQHAHGPRESVIVEGARAWIGSAAHCEVRLPIDQAAP
                     EHVLVELQGGGAQLHVHADAEAPRVNELPLSAADLVPGSVLQIGQVRLAVSWAEDDRT
                     GPNPESTARSNREVLFALGLVLLFLVLGFGMLSERATPLEQAPNTYPPLFAAPAASCP
                     DVDPGRARARGEEYLAVADTKRERMPFAIDEGVAAVGRYETASACFRVGAIEGRAREA
                     QQSADALKEQLSNEFRARRLRLSHLLRVQDRELAAEDVRALRQLLRAHQGPYVDWLEA
                     VSRQLESRSEE"
     gene            14045..14677
                     /locus_tag="VFQ61_13925"
     CDS             14045..14677
                     /locus_tag="VFQ61_13925"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9955606.1"
                     /translation="MSRRPRWPRADSAHRFRQALVFTIWLASLLPACAAPESSRRPAR
                     PPELEPKVLLARAEVARRLGDWTRAEQYYVVALESGGPPRWITQRLLEVCVADQRFPV
                     ALEYAEQYLARHPRDPELALAAASLHLALGGLDRARLLLEDILRSRPNWPEPHFALAS
                     VLRASERESGAPASGLADQHDFEYLKLSPTGPFAETARARSAPHEQLPIQ"
     gene            14674..15801
                     /locus_tag="VFQ61_13930"
     CDS             14674..15801
                     /locus_tag="VFQ61_13930"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9955607.1"
                     /translation="MISSPVFEQTLLEFFAPVLPFLQDPHVSEILINGPTRIYVERRG
                     QLELTAAQFPNREALHAALNNLSQYVGRPLNTECPLLEARLPDGSRVQAILPPIAADG
                     PHVAIRRFFKDTLTMSRLVEIGALSQVAATTLERCVAAQLNILVAGGTGSGKTSFLNA
                     LSGLIPDRERVVVIEDSREINFQKAHVVQLEARPADARGRGALGIRELFRATLRMRPD
                     RVVIGEIRGGEALDIVQAMTSGHGGCMGTLHASYPKDALTRLETMALMSDIDLPLAAL
                     RLQIGSGVQLLVQLSRLADGSRKVTHLTEVVGFDPGTQKYVLRHLFERRYRLPEARGG
                     EPVSDLLPTHELPCLQTRLLERGVTLPESLLHVHPSRAAGE"
BASE COUNT         2911 a         5241 c         4968 g         2778 t
ORIGIN      
        1 gagcgccgcg cgtgaacggc gcgtgatgcg cgagagtaaa cgcgtcgagc catcgccgag
       61 gatgagctgg acgttgactc ggctcgggcc gtcgtcggag atttccacga cgcgcgcatg
      121 tagcaagttg aggatgctgg tcggtgcggg ccgttcgcgg gaaaggctca cgtcccgcgc
      181 caagatgcgc gcgcggagtc gggtcccgag tggtcgctcg atgcgcgaga cccacaggtg
      241 ctcgttgccg atttcgatgc gcgtgagttg gtcctccggg tcatgcgcgc tgatctcgcc
      301 ggtcagaatt gcgctcgctt catccgagtg agcggtggga acgtcgagtc gagtgagcac
      361 ggactcgacc gggccgctgg cgagcgcatg gcctgactcg atcaggacta ggtgatcggc
      421 gaggcgcgcc acttcctcga tcgaatggct gacgtagatc atcggtaagg agagctcgtc
      481 gcgcagccgc tcgaggtacg gcagaatctc ggctcgtcgc gcccagtcga gctcggccaa
      541 aggctcatcg agcaagagca actcgggccc tcgcaagagt gcgcgcgcga tagcgatccg
      601 cttgcgctcg ccccccgaaa ggcgatcgac ggatcgcgag agcagctgcc cgatgcccaa
      661 caattcgagg atttgttcga agggcagtcg cagccgggcg cgtggattgc ggcgataacc
      721 gtattcgagg ttggcacgca cgtcgagatg cgggaacagg ctggcttcct gaaagacata
      781 ccccaccgga cgctggtgcg gcggtacgaa aattccccgt tcggagtctt gatacacacg
      841 gccgccaaac tcgacccgag ccggccagcg ttcgaggccc gccagcaaac gcagcaggct
      901 ggtcttgccc gaacccgagg ggccgaacag cgcacaaatg cctgaaccgg gcaactcgag
      961 tgcgagctgc aagtgcagcg aacccagagt gacatcgact cgcacgcgcg agtcagcggg
     1021 cggagagata acgcgatcca cggaaggaat tcagaacgaa tagaaccacg atcgaaaaca
     1081 cgaccagcaa gccagccagg cgatgagctt cagcatactc cagagcctcc acgtggtcgt
     1141 acaagctcaa ggagaccacc cgagtctgcc ccgggatgtt gccaccgatc atgagcacca
     1201 cgccgaactc accaaccgta tgagcgaagc cagataccgc cgccacgatg aagccgggac
     1261 gcgcgagcgg cacgaccaca ctgaagaagc gatcccaagg gcctgcaccg agcgaggcgg
     1321 ccacctcgat gggccgggtg ccgatcgact cgaatgcgtt ctgaagtggc tgcaccacga
     1381 atggcatgga gaacacgacc gagcccagtg tgatcccggt gaacgtgaac gcgagcgggc
     1441 cgagcccgag cgccgtcgtg aaccgcccca gaaagccgtg cggccccaag ccgagcagca
     1501 ggtaaaagcc gagcacgctg ggtggaagca ccaacggtaa accgatcact gacgcgaccc
     1561 agttgcggaa cggtgagcgc gtggtcgcaa gccagtatgc gatgggtgta ccgagcagga
     1621 tcagaatcga ggtggtgagc gtggcgagct cgacggtcaa gcgaatggcg gtgaggtcgt
     1681 cgtcgctcat cgaatgcgcc cagtgtatcc gacgtgctcg atctcacaag cgctgcggca
     1741 cacggggcgc aggtttcagg ggctttgcgg gcggcaccaa acggctgaat ttctgctcga
     1801 tcaacacgag ctcggagcga tcggaggcta cgaatgtcag ctccgggttg cgcggcagca
     1861 aggacgcatc tcgcgtcgta tccaacgtga gcgtgacggg ttggcccgcg agcacgctga
     1921 gttcgaggtg ggccacgccc gaggagtcgg ttttcgcgac ggtttgattg aggtatcgaa
     1981 tgggtaactc tccagcattg gaagcgcgca acccgaccac cacgcgacgc tccacgcgtg
     2041 tgcatgcagc atcgaagcgc ggaatcagtg cggcgtcgct cagggcatgc agggtccagc
     2101 tgagcttcga cgtcgaggag ccgtagccgg cggggcagtg aatcgcgatc tccgcgcgct
     2161 cgccctcctc acccgtgagt gagacgcggg cgattccaga ctcggagctc gtcgcgacgt
     2221 gctcaccgcc gcgccaaagc tgtgcgcccg ccaccggctc cgggccggag tggacccgaa
     2281 cctcgatcgt aaagcgggag cgaaggtcgc ccggaggctc gaccttcgca caagcaatca
     2341 acccgtgaac gaccccgaga gtgcagacaa gcttcgcagg cctcccgctg acggcatggc
     2401 aggatagccg cttcgagcac cgagcgggag agcttgaatc ggcggtcact tccgagtgcc
     2461 ttccgcagac gagcgctggg cctcgaacgc tgcgctgagc agctcgcccg ctgccagttc
     2521 ggacaaggtc accgtcgttc gcgcatcgtg ctcatgagtg gcgacgagga gctgcgcgcc
     2581 gttctcgctc aggtaactcg tcgagtttgc gccgcgcgtg actcggcgta atccgcgggg
     2641 ctgcgcccag cgttcgtaga aatcttggac gacttgggct cgctctgcgg agtcgtaaat
     2701 ccgcacggcg agcggcagcg cctccgccgc cgcactgagc aggcgtaggg cattgggcgg
     2761 ccggggcacc caacgtgaat cgctaccgct cgcgtcctgg tcgccgggaa acatgcgtcg
     2821 cacattgacc ggatcatcgg tccagagggt aacggcacgg gtgccggtag gtacgcgctc
     2881 cgcgtagaaa tagcggaggt gtccgaaggt cgccagatcc gggttggcca cgaactggct
     2941 gagtaccgta gtaactgcaa agccacggcc gccgaaacca gctcctttcc cgacgaagca
     3001 aaccacgaag ccgctggcgc cagcgtcctc tcgccaaact ccgtttcgcc acgtgcggtg
     3061 actctccacg ccggagtcgc gcttcggttc tgatcgggtg agccactggg agagctccgc
     3121 cagaacgccg ggtcgagcca gacagtgtga ttcgaatcga tcgagcgtcg cagacaccga
     3181 gttcgacgtc accaacgacg cgtgtctgac gtgaacgcca ttcagctcga tggcctccgc
     3241 gcgggacgta agctcggcca aacccagcag ctcgtgaccg accgcgagca ccccttcttg
     3301 cgcgtgagcc cacacgcacc gcgcggccca tgccgctccc agcacgaaca cgcaagccca
     3361 atacagagcg agcctgagtt gtgcgccagt cggcagaagc cgatgaacac acattcggaa
     3421 tctgccatgg aaagcaattt cgctgattct gttcatgtcc ctactcgata ttgaagactc
     3481 gcctatggtc cgattgacgg gcagaacgca gatagaggtc agcgcgtcag tgggccccgg
     3541 caacgaaggt cgagaactct cccttcagcg aactcaacag ctctatccgc gcgtcttccg
     3601 caatgggatc cgcgcaactg ccgtacgagc gagcgacgat cgactgagag agcacacgat
     3661 gcaccccttc gctctcactc gatacgtgga ctcgaccggc gagcgccgcg cgcgtgatcg
     3721 gatgggccaa acgccgaccg gcgccttcgg ctcgactgcc caaacgagct aagaagcgct
     3781 gcgcgttcgc atcgctcgtg ccgctcgcgg cctgcgccga actgctcacc acctcggggt
     3841 cttgtccgct cccaagcact tcgaagcgcc cgcgatcgcg ccccagccat gaacgcggct
     3901 cgttcgtgcg gcagccgctc atcgcgtggg ccaacacggc cgcccgagcc aaacgcatgg
     3961 cgctgagctc cgagacgtag acttgccgaa agaacgccaa tcccaagacc cccagcatca
     4021 aggacgagat cacgaccatg gcttccacca aagccgctcc gcgctgctgg gcccgagaac
     4081 atcgtgcaga ttccaatgaa tcaccggatt tcaatggatc accggctccc ttgccgcgcg
     4141 actcagctgt cggagaagct cggcgttgcc cggcgtcggc gcgccgcgct ccgccaggcc
     4201 ctgaacggac cgggctatcc cttgggatag gccaagcaac tgcccaaagc tctcgattag
     4261 cttttgttcg cctcccgagt agcgccgcag gcgcgctcgc caacgcaggt gccacatact
     4321 ctcgctatcg cccgcgtcac agtcggcatt cgaccagggc ccggcacaat cgaagaagta
     4381 ctccgcctgg gcaaaggcca acgctggcgg aagcgactcc aactgtttcg agtgggcggg
     4441 ggcggctacg ctcacgccgt ggagcccacg actcaatagg tcgcgccttc cttcgctcag
     4501 cgcgagcaac tgcccgaccg cgttcccgtt ctggaaacgc gactggatag cccacggccg
     4561 cagctggtcg cgctgggagt cggagctctc cctgtcgccc cccagcccgc cgagctcgtc
     4621 ttgcgcgcgt tcggcctgtg cgcgcttgcg gcgctcgcat tgctccgcgt cgaagtgacc
     4681 gagggcggcc tgcgcggtac gcaaggacag ctcgagctcc gtgagctccg cgtgctcagc
     4741 gggtgtgggc gggcgcgaac ctccggcgca cggcgcggaa tagcgggcac aggcctcgag
     4801 atagctctct cgcgcctgat cccgacgctc ggtgatgcca cgccgctcct cctcacaggc
     4861 ctggccgggg agaccttcga gctcttcgga gaagtccggt gcagcgccga agcccatctc
     4921 gcagaaatac ggcccacccg ccgcaacggc cttaccgacc agcgcgctcg cacgctcgaa
     4981 gaatgcgcga tctacaggca agtgcaggag ccacgtcacg atttgcccaa ccgattcgcc
     5041 ggattcccga caaagccgcc caggctcgtc gtaacgaacg ggcaggccgc ccaacgaagc
     5101 cacgcccgcc gccacggtac tcgagacgag cggcgcgaac cgccgcccca attgcgccga
     5161 tccggcgatg gctccggccg gtgtgagctc cctaaccact ccacccagtc gactcagcgc
     5221 ggagattgat cgattgatcg gttcgcgcgt ggcgttcacg gcactctgca tctgcgcggc
     5281 cccctcgaag cagagcgcgg ctacgggttg ccccgcgaac ggcaggagcg acgctacgat
     5341 accggccacc gtgagcccag cctggattgc cttcatcgcc acgcgcagcg ccaagatcga
     5401 cgccatcagc aagttcaaca gcacgattag gttcatgccg cgagcatgca ggacggcggc
     5461 gctgaacgcg gcggcatcgg cggactcctg caagcgctca cgcaacagca aagcatcccc
     5521 aatccccgcc acgtaccaaa ggatcccgac catggcgctc gccatgaaga tccctaaaac
     5581 gaggattgcg ccccgctcat cgttgtcgag tgtccgcgga gtgaagactg gggacgtccg
     5641 tcggcagccg tcgctcggtg ccaggtcatt tgctgtattc atagttggct ccctgataag
     5701 gaaacacggc ctcggcccgc acggtgcggc tcgggaggcc actcctgcct tttgctgcgg
     5761 agcagacaat tcgatcggcg agaccgatta ggcacacggc gcgtgcctgg acattcaccc
     5821 tcaccttatc cacgcttgct ccgtgcatcg gccgcagctg cacccgatcg cgccgagccg
     5881 cgactccttg gcgcgcggat tcctcgggca atcccagctc cagctgatcc agcgttccgt
     5941 tcaaaaccca ggggctcaag ctcaacagag ccgcgtcgaa gacggctcga tagcgccggc
     6001 ccgttccaat ctgtaaagag tgcgcggctt ctcccgcgta gcgcttgggg tcatcgccaa
     6061 tcacgacggc ggccgcacgc gcagcgttgg cagcagcatg ctccacgccg agtcgcactg
     6121 caaacaatag accgagctgg atcacacaca ggaagaagac atacacgggc atgaaggcga
     6181 gcaagaactc gacgaacacc gccccggtgt catccaaaca ccgagtcagc gcgcaatggt
     6241 tggcggtcgc tggcgggtgg cggccacttg ccaatgccaa agagggttca acggtgcagt
     6301 tcgtctcgaa tggagtcatg accgcagagc ggtgcaacgt tcgtgcagac cgggctggcc
     6361 ttgggtcgga cgagcaaagg ccggcaactc tcggccaaaa gcggctggat cgtgggcagc
     6421 accgcacctt cgcgatcgca atcactccag ctggcaccgg aattgctcaa gccaccccag
     6481 acaggcgaat gcgaatgtcg aagcctactg tcgagaaacg gtatcatcac tccgcgattc
     6541 tgagggatga gcgagcctcg gccagcgtcg aatatctcgt attggtgggc gcggtggggt
     6601 tgagcgcaag tgttggaatg gtcgcggtgg gtgttgcaat cctacgcagc ttcgacttcg
     6661 tgcgcgccct gttattgagt cccattccgt agtcatgccc tcgatgggaa gccgtcgcac
     6721 tccggagcat gtctcacgcg cgacccgccc gatcgaagag gcatgcaagg agagtccatc
     6781 gaaatgccag cccaagaacg gaccaccgcc cgccatcggc gcggcgcgac catggtcgag
     6841 tacatcatcg tcatcggcct ggtcgctctg ctcgcgatcg cggcattcaa gttcttcggt
     6901 gaaaaggtgg ggaacaaaat caaatcccaa ggcaatgcgg tggaaaagat caacgacacg
     6961 gcgccttgag cgcggccaat gaaactcgac taatccaatg ttgcccttcc tctgcgccac
     7021 cctaatccta tgcttcgtct cgacgattac agacttgaga tctgcacgaa ttcccaactg
     7081 gttgactttg ggtggcgcag cgctcgggct aatcggccac ggcgctctcg gctggagctg
     7141 gggcggctat ccggcgcttg gcgaggcact cgcgggcagc ttgagtggcg gcctactctg
     7201 cgggctcgta ccgctcgttc tctacctgaa gggcggcttg ggcgggggtg atttgaagtt
     7261 gttcgtggcg attggcgtgc tctgccgtcc cttgctcggt attgaaatcc agacctatgg
     7321 cctagttacc gcggccgtca ttgctcccgc gcggctggcc tacgaggggc ggctcttgcg
     7381 agtggcgctg gggtcattgc agctcgcgac cgacatgttc cggccgaagg agcgccgtcg
     7441 agcgccaccg ccggaactgt tggcctcatt tcggctcgcg cctgccgtgc tcgtcggcgc
     7501 actgctgagc ctcattcatc acagctacga gctactcccg cgctgatcgg agcgacatga
     7561 gggcacgact atcgaccatg aaactgagtc caacttgggt aggcctcttg ctcgcgaccg
     7621 cgggggttgg cctccaattg ctgtacatgc ggaggttcga ggcggatgtc tcgggcggcc
     7681 gcaagatcga gctcctggtt gccgcccaac ccctcgaacg cggcaagccc gttcgtgccg
     7741 agatgttggg ggtgcgcgcg gttccccaag cctacgtgga cgaccgcgcc gtgagggccg
     7801 aagatcgcga taaggtcgtg aatctgcgcg cgaccaacca ggtgccggcg ctccaaagcc
     7861 tcgcctggac cgacttcatc gcggtccacg acgaccagcg cgacttgagc tcgctcgttc
     7921 agcccggaaa ccgcgccatg ccgatcaaag ttcagtcgga ggagtcgctc gagctgatcc
     7981 gcccgggcga cttcgtggac gttctcgccg tcgacgcaga cgggcgcgag gccagcgtgc
     8041 tgctccaacg ggtgctagtg ctggcggcgg ggctcgagac gagcgtcgag cgatcttcag
     8101 agaaatccac gggcccgtcc gctcgcctcc tgacggtgag cgtgactttg gcggaagcgc
     8161 aactcctcgg gctcgcccag acaatcggca gattgtgggt cgtagtacgc ggcgccgagg
     8221 acccgcgagt gttcgagtcg ccacccgatg tcagccgcag cgatctgttc gacgcacacg
     8281 tgcgccagaa cctgcagcga aacgcgcgac acccggtgcc gatccgactg gagccggagg
     8341 tcccatgaac gggagcccaa gtgcgttcgc agagcggcct cgcgctcggc cccgcgccgc
     8401 gtactcgaaa ctgctgtccg cctcattgtg ggtttcggcg gtcctgctct ttcctagcgt
     8461 cagccctgcc ggtagctcac aacgcggctc gagtgaagcc gtcggcgaag tgtcgctgtc
     8521 ggttggtgag acacgcactc tgccagctcg agacgtcaag aactactccg aaggcgtgca
     8581 gggagtcatc gatatcaaac tgacctccga tgcttctcag ttcgtactgg tcgggcgcaa
     8641 gccgggctcg accactttgc tgctgatcaa gaacgacggc agccagtctt cttggaacat
     8701 tcatgtcttc tcgcgcgcgc ccgaacgtgt ggagcaggag ctacgcgagc tattgaatgg
     8761 tctgaacgtc cgctcacggc gcattggctc ccaaatcgta ttggatggcg tggtggccaa
     8821 cgacactgag ttgagccgag ttcagcgcat caccgcgctc tacccagagc aggtcatctc
     8881 actcgtccag ctcgccaccg aaggcgttcc ctcacctcaa tcgggaaatg ccaaatatct
     8941 aatccgcatc gacttctatt tcgtgcaata cgacaaaaac tcgagctacg gagtgggcct
     9001 cgcctggccc gactcgatcg ccgccggagc cgtcgacttc tcctacgatt tcttggccaa
     9061 cacggcgcgc gcggccaccg caagcgtcag caagcagccg ctaccctcgt tggatttggc
     9121 cgctcggcgt ggctacgcca aagtcttgaa gcaagcggcg gtggtcacca acaacgataa
     9181 cgaagccaat ttctcgaacg gcggagagca gaacttcacc gtaaacacag ggctcaccgt
     9241 cggggtgcag cggatcccgt tcggcaccga catcacggtt ctgccgcact atgaccccga
     9301 cgccaaaacc ctaagcctga aactggccgc tgaggtgagc gacctcacgg cgtcagtgtc
     9361 tggcaccgcc ctaccgggtc gttcgacctc gcggctcacc acccaagtca gcctgaaatt
     9421 ggggcaatcg ctggtgctct cgggcatcca cagtcactct ttgacccata gtgtttcggg
     9481 gctccccggc ttgagccaaa taccgattct cggaattcta ttcggttcgc atacgcaagc
     9541 ggagctggag acagaggggg ccatattcgt agtaccaagt gtcgtgcaga gcctgccaaa
     9601 ctcagagcgg gacttggtgg atgcggcctt gaaacgtttc gagacgttcg acggcggcga
     9661 tctgggccag cttcgctcct tcgatcgcac cccgcactcc gaacacgggc gggcctcgcg
     9721 aggaacccat gagcggtaac gacgaagttg gcagcatccc ccccgtgcaa caccgacggc
     9781 tccgcttgag ccgaggcgaa ccgtgcacga tcggtcgcgg cctcgaaaac acgctcgttc
     9841 tcgatagtcc gcgggtttcg cgccaccacg ccaccttgga actcctgggg tcccagctgc
     9901 gtgtcgccga ctgctctcgc aacggaagcc tggtcggtga tctgtatatc caacacgccg
     9961 aagtcgcagt ggatccgcgt actcccattc aaattgggga attcgtcctc acgttggagg
    10021 acgaaactgc gtccaccgcc gagttggatt tcgtcgccgt gcccgcgcaa ccggccagcg
    10081 ctccatggcc gatcgagtcc ccacggacta gtctcgagct gcggcgcgag gtccatcgct
    10141 tgctgctcga tcatctggat ctgtcttcca cgcgtgcggc gcgcctcgaa gatccgacgt
    10201 tgcgctcgcg agttctctcg gccttgcggc gaatcatcca cggcctagaa gcacgcctcg
    10261 accccggcat cgaccgagat gcgctgatcg gggaactggc cgatgaagcc ctggggctag
    10321 gcccgctcga agggctgctg gccgacccgt ccatcaccga gatcatggtg gtcgatccgg
    10381 acacgatcta cgtcgaacag cgcggcaaga tcgtgcgctc gcgcgcgcgc ttcacggacg
    10441 acgatcgagt gcgggccatc atcgagcgca tcgtcacccc gttgggccgc aggatcgatg
    10501 aatcgagccc gatcgtggac gcgcggctgc ccgatgggtc gcgggtcaac gccatcattc
    10561 gtccgctcgc actgcgcggc tcgtcgatca ccatccgcaa gttctccaaa gcgccgctgg
    10621 ccttgaccga tctagtccag ttcggtgccc tcacccctgg catggggcgc ttcttgacgc
    10681 gctcggtggc ggcgcgcaag aacatcgtga tcgccggggg caccgggagc ggcaagacca
    10741 cgcttctcaa cgtgctgtcg atggccatcc ccgcagacga tcggatcgtc accatcgaag
    10801 atgccgcgga gctcaagttg cggcagccgc acgtcgtctc gctcgaaacc cgcccttcga
    10861 acatggaggg taagggggag tattcgattc gcgatctggt ccggaatgcc ttgcgcatgc
    10921 ggcccgatcg catcgtcgtg ggcgagtgtc gcgggggcga ggcgttggac atgctccagg
    10981 ccatgaacac gggccatgac ggctcactca ccacgacgca cgccaactcc ccgcgcgagg
    11041 cgttggcacg tctagagaca ctcgtgctca tgtctgggct cgacttgccc gtacgcgcca
    11101 ttcgcgaaca aatcgcctcc gctatccacc tgctcgtcca gcagtcgcgg ctctcggatg
    11161 gcagccggaa gatcacttcc atcgccgaga ttgcggggct cgggcccagc ggcgaaatcg
    11221 aggtggtgcc gctcttcgag ttcgtgcgta acggcacttc gagcagcgga gaggtgcgtg
    11281 gagaattcag ggccaccggc tttctgccca gcttcctgga tcaattccgt gtgcttggcc
    11341 tgattcaacc cggcgagcct tacctatgag gttgccctat tccgtggaga ccctcgagta
    11401 cttcgggtta gcgctgacct tcagcggcct actactcggt tcgcgtgccg cgctggcgcc
    11461 cggttcgggg gcgcggcgcg cgtatcgcgc ctataccgcg gaactcgacc gcaagctcgc
    11521 ggcctcgttc ctctgcggct cgggcccgcg gatcgcggcg ctgcaactcg cgctgggagt
    11581 aggggtagtg acgctcggcc tcggcttggc aatgccgtac ggatgcgtgg cgctcggccc
    11641 gctcgcgctc gctcccaact ggtatctcga tcgcaagcga agaatccata tctcatcgat
    11701 tgaggcgcag atcgatggct ttattctcgg actggccaac gctctgaaga gcgtggccag
    11761 ccccgccgcg gcaatcgccc agctcggtcc ggccttgcca ctgccgatgc gtctcgaagt
    11821 cgacaaagtg aatcgagagg tgcggctcgg aagtagcctc gatcgcgcct tgctcaatct
    11881 atcttcgcgg ctgcgtagct ctgaagtgga tacggcgctc tcggcggtcc tgatcggcct
    11941 ccagattggc ggcaacttgc ccagtgtctt ggagagcacg gccgccacgt tgcgcgagat
    12001 gaagcgcctc gatggcgtga tccgcaccaa gaccgccgag gggcgcgccc aactttgggt
    12061 attggcgctc tttcccttcg tgatttgctt gggcttcagt gcggtcgacg agcaatactt
    12121 cactcccctg cgccacggtt ggcttggcag cggcctcgta tgcctcggac tggggctttg
    12181 gctggtctcg ctgctgctgg cccgtcagat cctcaaggtg gagctgtgat cgcgatgttc
    12241 agcccgctga gtatcttggc ggtcgcggcc tgggcctgcg tgttctgcgg ttgctttacg
    12301 ctgggttggg tggccgcgcg agcccaattg ccggagcggc gccggctcgg gctgcgtggc
    12361 gtgaagcgag ctgcagctct cgcggagcgg cccgcctggt ccgccatcga gccgtcggta
    12421 cgctggcttg gtgcgcgcgt ggtgccttgg ctcgggcccg cgacgcgcgc acggctggat
    12481 cgccaactta ccttggcggg cgacgctcta gggcttcttc cggaggaggt cacggccatg
    12541 agcttgatgt gcgggctagc gggcggctcg atcgccaccg tactcggttg gatgacgggg
    12601 cttggcccta ccatgacctg ggtgggcgcg ttgctcggtg ggctcattcc catctgggtt
    12661 ttgcaaagca cggggtccga tcgcgtccgg tacatcgaac gtcgtctgcc gcacgccatc
    12721 gatctgctgg cactcgccat gggtgcgggg ctcgatttcc ctggctccat gtcgcgcgtc
    12781 gtcgagaaag cgggcacccg acgtgatcca ctggtcgaag agttcgcgct cgtactccag
    12841 tgtttgcagc tcggacaaac tcgccgtcaa gcgctcgagc agttcgcggc gcgcacgccg
    12901 agcgctgcgg taaccgagtt cgtggcggct ctaattcagg ccgagttgcg cggccatccc
    12961 gtcgccgagg tgctgcgaat tcagtcggag gtggctcgcc gtaagcgcag cctgcacgcg
    13021 gaggaatccg cagctcgcgc cggagtcgcc atgatgggtc cgctggtatt gatgttcttg
    13081 ggcattctcg tcctgatcgt ggggccgatg ctgattcggc tgtctggtcc ttagaacctg
    13141 cctcagagcg accgtgtctc gtcccccgct cgattgagca caccatgacc accctcaaat
    13201 tcgatatcca acatgctcac ggcccgagag agagcgtcat cgtggaggga gctcgcgcct
    13261 ggatcggctc ggcggctcac tgcgaggttc gcctgccgat cgatcaagcg gcgcccgagc
    13321 acgtcctcgt ggaactacag ggcggtggag cccagctgca cgtccatgcg gatgccgaag
    13381 cgccccgcgt aaacgagctg cccctcagcg ccgccgatct cgtcccaggc tcagttcttc
    13441 agattgggca agtcaggctc gcggtatcgt gggcagagga cgatcgtaca ggtcccaatc
    13501 ctgaatcgac ggcgcgttcg aatcgagagg tgctgttcgc gttagggctg gtcttgttat
    13561 tcctcgtgct cggctttggc atgctgagtg agcgcgcaac tccgctggaa caagccccca
    13621 acacctatcc gcccctattc gcagcacccg ctgcaagctg cccagacgtc gatcccggcc
    13681 gcgcccgcgc ccgcggcgaa gaatacttgg ccgtcgcgga caccaaacgg gagcgcatgc
    13741 cattcgctat cgacgaaggc gtagcggcgg tgggccgcta tgagaccgcc tccgcctgct
    13801 tccgcgtcgg tgcgatcgaa ggccgcgcgc gcgaagctca gcagagcgcg gatgctttga
    13861 aggaacagct gtcgaacgag ttccgcgcgc gcagactgag actgtcgcac ctgctgcggg
    13921 tccaagaccg cgaacttgcc gcggaggacg tgcgtgcact gcgacaattg ctgcgcgcgc
    13981 accaaggacc ctacgtggat tggctcgagg ccgtctcgcg ccagctcgaa tcgcggagcg
    14041 aggaatgagc cgaagacccc gctggccacg cgccgactcc gcgcatcgat ttcgccaagc
    14101 actcgtgttc acgatctggc tcgcatcgct cttgcccgct tgcgccgcgc ccgagtcgag
    14161 ccggcgtccc gcgcggcctc cggaactcga acccaaggta ctcttggcgc gcgccgaggt
    14221 ggcccgccgc ctcggcgact ggacccgcgc ggagcaatac tatgtggtcg cgctcgagtc
    14281 aggcggcccg ccccgctgga tcacccaacg actgctcgag gtgtgcgtcg ccgaccagcg
    14341 cttcccggta gccctcgaat acgccgagca atacttggcg cgccaccccc gtgatccaga
    14401 gctcgccttg gcggccgcgt cgctccactt ggcccttgga ggcctcgatc gggcgcggct
    14461 gctgctggaa gacatcctcc gcagccgccc caactggcca gaaccccact tcgcgctggc
    14521 aagcgtcttg cgcgcctccg agcgagagag cggcgcacct gcgagcggcc tcgccgatca
    14581 gcacgatttc gaatacctga agctctcccc aacgggacca ttcgccgaaa cggcgcgggc
    14641 ccgcagcgcg cctcacgagc agctcccaat acaatgatta gctcacccgt gttcgagcag
    14701 acgctgctcg agttcttcgc tccagttctg ccgtttttgc aggatccgca cgtgagcgag
    14761 atcctcatca acggtccaac tcggatttac gtggagcgac gcggtcagct cgagctgacc
    14821 gccgcccagt ttccgaaccg agaggcgtta cacgccgcgc tcaacaatct gtctcagtac
    14881 gtgggcaggc cgctcaacac ggaatgtccg ttactcgagg ctcgcctccc cgacggctcc
    14941 cgcgtgcaag ccatcctgcc accgatcgcg gcggacggtc cgcacgtcgc tattcgccga
    15001 ttcttcaagg acacgttgac gatgtctcgg ctcgtcgaga tcggcgccct ctcgcaagta
    15061 gcggcgacca cgctcgaaag gtgcgtggcc gcgcagctca atattctggt ggccggcgga
    15121 accggcagcg gcaaaacctc cttcctgaac gccctctccg gcctgatccc ggaccgcgag
    15181 cgcgtggtgg tgatcgagga ttcgcgcgaa atcaactttc agaaggcgca tgtcgttcaa
    15241 ctggaagcgc ggcccgcgga cgcccgaggc cgtggcgcgc tcggtattcg agagctcttt
    15301 cgcgcgacgc tccgcatgcg ccctgaccgc gtcgtaatcg gggagattcg aggcggcgaa
    15361 gcgctcgata tcgtgcaagc catgacctcc ggccacggcg gctgtatggg caccttacac
    15421 gcgagttacc ccaaagacgc gctcactcgc ctcgagacca tggcactcat gagcgatatc
    15481 gatttaccgc tggcggcgtt gaggcttcaa atcggctctg gggtacagct cttggtacaa
    15541 ctgagtcgtc tggccgacgg cagccgtaaa gtgacccacc tcaccgaggt cgtcggcttc
    15601 gatccgggga ctcagaagta cgtgctgcgc catctattcg agcgtcgcta tcgcctcccc
    15661 gaagcccgcg gcggcgaacc cgtgagcgat ttgctgccca cccatgagtt gccgtgcctt
    15721 cagactcgac tgctcgagcg aggcgttacc ctgcccgaat ccctgctgca cgtgcatccg
    15781 agccgcgctg ctggcgagtg agcctccctc acccatggag agcaccatgt tcgacgatcc
    15841 accgacccta ccgcttcaac gcccccagcc acccctattt gcgaacccgg aagtcacg
//
LOCUS       DASTKU010000404         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_75758,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000404 DASTKU010000000
VERSION     DASTKU010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75758"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFQ61_13935"
     CDS             complement(<1..664)
                     /locus_tag="VFQ61_13935"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HET9955608.1"
                     /translation="MTRPAPAWLASFQAEFARALCLPLDRSKGRLEARTGEYATAWLG
                     QARTTARVPAAERVAVYNRQYWFRLFTALQTCYPLTARLFGFWEFNALGARFLQKQPP
                     KSWDIDDVPREFDQFLSAELCQSEELCQSEEPRLGQSLEFPERAALIEAARIDAAHHE
                     VLRAPRVEVYRPVAVSAGELSKLHLRPSPHWRIVVEHWPLVELRRELLRDRSEHRVPL
                     PHA"
     gene            complement(661..1488)
                     /locus_tag="VFQ61_13940"
     CDS             complement(661..1488)
                     /locus_tag="VFQ61_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HET9955609.1"
                     /translation="MTRSPPAIRRDALGLGLRRPHYGYIFEHWPAVDFFEIISENFLG
                     PAFLPQYHLSRVRERYPVVLHGVGLNLLGHAPLDESYLDALCRLAEASGAAYVTDHLC
                     WTGAHGVQHHDLLPTPYTEELVEFAAERAAYVQGRLGVPFGLENLSSYVEFESSTLSE
                     AEFYTRVVKAAGCWSMLDINNVYVSSQNHGFDPSSYLELVDFTRVLQVHLAGHTRTAN
                     GLLIDTHDHPVSNDVLALYRVAWQHGGPFPTLLEWDDQLPPMPELLGELERIREARA"
     gene            complement(1569..>2021)
                     /locus_tag="VFQ61_13945"
     CDS             complement(1569..>2021)
                     /locus_tag="VFQ61_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955610.1"
                     /translation="ELETYIVMWDRYHPYVGEHLVYRYSDPMFGGRHALVDYDQCRYS
                     PMMLFFARKAKTLPEIVIPLQFEGNYVAGGEVPAGEHGAHAKAPPHSEDSVTPQTSVT
                     PPRSAKQRLAKLVLSAISRLPEEWAYSMNARLRRWRLLKHTRKASFRF"
BASE COUNT          414 a          624 c          627 g          356 t
ORIGIN      
        1 tggcatgggg cagcggaact cggtgttcgc tgcggtcccg cagcagctcg cgacggagct
       61 cgaccaaggg ccagtgctcg acgacgattc tccaatgggg actcgggcgc aagtgcagtt
      121 tcgagagctc acccgcgctc accgccacgg gcctataaac ttccacgcgt ggtgctcgga
      181 ggacttcatg atgggcagca tcgatacgag ccgcttcgat caacgccgcc cgttctggga
      241 actctaagct ctggcccaat cgaggctcct ccgattggca gagctcctcc gactggcaga
      301 gctcagcgct caagaattga tcgaactcgc gagggacatc gtcgatgtcc cagctcttgg
      361 gcggttgctt ctggagaaat cgcgccccga gcgcgttgaa ctcccaaaaa ccaaacaatc
      421 gagcggtgag gggatagcag gtctgcaggg cagtaaatag ccgaaaccag tactgccggt
      481 tgtagacggc gacgcgctca gcggcaggaa cgcgcgcagt cgtacgcgcc tgccccaacc
      541 aggcggttgc atattcaccg gtccgcgctt ccagcctgcc cttcgagcga tcgagcggca
      601 agcacagggc acgggcaaac tcggcttgaa agctcgcgag ccaagccgga gcgggcctcg
      661 tcatgcgcgt gcctctcgaa tccgttcgag ctcgcccagc agctcgggca tggggggaag
      721 ctggtcatcc cactcgagca gcgttgggaa gggtccgcca tgctgccagg ccacgcggta
      781 cagcgcgagc acgtcattac tcacgggatg atcatgagtg tcgatcaaca acccattcgc
      841 cgtgcgcgtg tgccctgcga gatggacctg gagcacgcgc gtgaagtcca cgagctctag
      901 ataggagctg gggtcaaaac cgtgattttg gctggatacg tagacgttat tgatgtccaa
      961 catggaccag cagccagcgg ccttcacgac tcgggtgtag aactccgctt cgctgagggt
     1021 cgagctctcg aactccacat aactagacag gttctcgagc ccgaagggca cacccagccg
     1081 accttgcacg taggcggcgc gctccgcagc gaactcgacg agctcctccg tgtagggggt
     1141 tggtaacaaa tcgtggtgct gcaccccgtg cgctcccgtc cagcagagat gatccgtcac
     1201 ataggctgcg cccgaggcct cggcgaggcg gcacaaagcg tcgagataag actcgtccag
     1261 cggcgcgtgc ccgagcagat tcaagcccac gccgtgcaaa accaccggat aacgttcgcg
     1321 cacccgtgac aagtggtact ggggcagaaa cgccggcccc aggaagttct cgctgataat
     1381 ctcgaagaag tcgaccgccg gccagtgttc gaaaatatag ccatagtgcg gtcgtctcag
     1441 acctaacccc agcgcgtcac gacgaatggc ggggggactg cgagtcacgc tgagccaggg
     1501 catccgtgag tcgagagctt agcgcgcccg tcgcgtacgg gcgcgtgatc cccgcttcct
     1561 cagacggatc agaagcgaaa gctcgccttg cgtgtgtgct tcagcaagcg ccaacggcgc
     1621 aacctcgcgt tcatcgagta tgcccactcc tcgggcaacc gagagattgc ggagaggacc
     1681 agcttcgcta gtcgttgctt ggccgagcga ggtggcgtga ccgacgtctg cggggtcacc
     1741 gaatcctcgc tgtggggcgg ggccttcgcg tgcgcgccat gctctcctgc tggaacttcc
     1801 ccgccagcca cgtagttgcc ttcgaactgg agcgggatca caatctcggg cagggtcttc
     1861 gctttgcgtg cgaagaacag catcatcggt gagtaccgac attgatcgta gtcgaccaag
     1921 gcgtgtcggc ccccgaacat tgggtcagaa taccggtaaa ccaggtgttc gccgacatac
     1981 gggtgatacc gatcccacat cacgatgtaa gtttcgagct c
//
LOCUS       DASTKU010000405         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1190911, whole genome shotgun sequence.
ACCESSION   DASTKU010000405 DASTKU010000000
VERSION     DASTKU010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190911"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            440..979
                     /locus_tag="VFQ61_13950"
     CDS             440..979
                     /locus_tag="VFQ61_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET9955611.1"
                     /translation="MHPLHDILVTQSIEEFLPAVVEIPKGSKLKYELDKRTGLLMLDR
                     VLYSSVHYPANYGFIPQSYADDGDPLDILVLMQEPVVPLTIVRARPIGGFSMTDDKGV
                     DDKIVAVAVDDPAFSHLADVSELPLHIMREIKRFFEDYKALEHKMSVVEGLYDRKQAF
                     VVVRAALEQYRANTAWNKG"
     gene            complement(980..1723)
                     /locus_tag="VFQ61_13955"
     CDS             complement(980..1723)
                     /locus_tag="VFQ61_13955"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET9955612.1"
                     /translation="MALVEPQHALLQRTPGSHGSPPPAGAPEPSAPPSSTSIAPAAPA
                     NTAQSGARMGPWAKLIAKTCASLALLAGLAGIGAFVTWQQPQLRGMRIDARPSASGAW
                     LAASKPDKSNPGSEFTAQAAANPLAHESGPQVRAVVTSPASRLEPTVSPASSSAGSDT
                     TALPGLTADGKVILNLAGASELRRLPGVGAKRAEAILALRNKLGRFKSPNDLLRVRGV
                     GLRMLRRWLPLIVLDAPEPSTAGGSAQLR"
     gene            complement(1734..1985)
                     /locus_tag="VFQ61_13960"
     CDS             complement(1734..1985)
                     /locus_tag="VFQ61_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955613.1"
                     /translation="MENSRLKIAYVIAQRGGRNYWNRVGVAFLNNDGSLNVKLEAVPV
                     NGEVHIRDYVPREEFTPPPARERSGRGMPEEEEPIVELA"
BASE COUNT          398 a          736 c          744 g          462 t
ORIGIN      
        1 tcaaggtgcc actagacgcc ccgaatcgct cccgccgcct tcgatgcgga tcgttcggcg
       61 cctctcgatc aaggcccggc gcctgcctgg cggtgagcgc gtcgaccgtg cgggcgtttc
      121 ggtgaggtct cggggcaggc gtttacggtg accgctttta cggttgtcag cgttcacggc
      181 tttgagtagc gaagcaggct gggctcacag ggcgcaaaca ctgggtaatg acactggtgc
      241 caacaccggg cgccaacacc gggcgtcaac attgcccagc actatgcttt cgcaagcagc
      301 gcttccgcac tggtgagata cagctcgcgc gtgtgggcgg ccgataacgc gaatggctgg
      361 ccattccgtg cagatcgaaa acgccagatc ttggggcggc cgcgcttctt tctacaaacc
      421 caatgctagg atgcgccccg tgcatcccct tcacgacatc ctagtcactc agagcattga
      481 agagtttttg cccgccgtcg tcgagatccc gaagggctcc aagctcaagt acgagctcga
      541 taagcgaacc ggcctgctga tgctggaccg cgtcctctac tcgtcggttc actatcccgc
      601 caactacggg ttcattcccc agtcctacgc ggacgatggg gatccgctcg acatcttggt
      661 gctcatgcaa gagcccgtgg tgcctctcac catcgtgcgc gcgcgcccca tcggtggctt
      721 cagcatgacg gacgacaaag gcgtggacga caagatcgtg gcggtcgccg tcgatgatcc
      781 ggcattctcg cacttggctg acgtgagtga gttgccgctc cacatcatgc gtgagatcaa
      841 gcgcttcttc gaggactaca aggccctcga acacaagatg agcgtggtcg aaggccttta
      901 cgatcgaaag caagcttttg tcgtagtgcg cgctgccctg gagcagtacc gagccaacac
      961 ggcttggaac aagggctgat cagcgcaact gggctgaacc gccggcagta gaaggttcgg
     1021 gtgcatccag tacgatcagc ggcaaccagc gccgaagcat tcgcagcccc acaccacgga
     1081 ccctcagtag atcgtttggg ctcttgaaac gcccgagctt gttgcgaagc gcaaggattg
     1141 cttccgcgcg cttcgcgccc acgccgggca agcgtcgtaa ctcgctcgcc cccgcgagat
     1201 tgaggatcac cttgccgtcg gcagtgagtc ccggcaacgc cgtcgtgtcg ctgccggcgg
     1261 agctcgaagc gggagaaacc gtgggctcta ggcgcgatgc cggtgaagtg accaccgccc
     1321 gaacctgtgg accggattcg tgcgccagtg ggttcgctgc cgcctgcgcg gtgaactcgg
     1381 agccggggtt ggacttatcg ggcttactcg cggcgagcca agcgcccgag gcgctggggc
     1441 gcgcgtcgat gcgcatgcct cgcagctgcg gctgttgcca agtgacgaac gctccaatcc
     1501 cggccagccc cgcgagtagc gccaagctgg cgcacgtttt ggcgattagc ttcgcccagg
     1561 ggcccatgcg agcgccagac tgggccgtat tcgcgggagc ggcgggagca atggacgtcg
     1621 aggagggggg cgcgctgggc tccggcgcgc ccgccggagg cgggctcccg tgggacccag
     1681 gcgtacgttg gagtaacgcg tgttgcggtt cgacgagagc catccctttt ccctcaggca
     1741 agttccacga tgggttcctc ttcctcgggc atgccgcgcc ccgagcgctc gcgagcgggc
     1801 gggggcgtaa actcctcccg cggcacgtag tctcggatgt gcacttcgcc gtttacgggt
     1861 acggcttcga gcttcacatt gagcgatccg tcgttgttca gaaacgcgac gccgacccgg
     1921 ttccagtagt tgcggccccc gcgttgagcg atcacgtagg caatcttgag tctgctgttt
     1981 tccatgtatt gagtcctatt ctcttgaagc gcgggctgtt gaccgcgcgc actcaggctg
     2041 attgcgagcg ccgtgccgct ttcggaaaat gcttgttgcc gcgagttcac ccggcatttt
     2101 ctcggtcgat cacagtggcg agcgctcgtc gggggtggcg tcgcctcggc caacgtcact
     2161 cgatctcaca gcatgcgcgc ttgcgcgctc cgccgttcac cgagcatttg cgttgcgctc
     2221 tgcacatggt tgcgctgaat tgagcgcgag attctagagc tcgtgatgtt gctctacctg
     2281 ttcgtgacgc ataggccgat tccgtcggcc ttcgaacccg cgggtcgcac gccttaggtg
//
LOCUS       DASTKU010000406         7591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1637185, whole genome shotgun sequence.
ACCESSION   DASTKU010000406 DASTKU010000000
VERSION     DASTKU010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637185"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VFQ61_13965"
     CDS             <1..1147
                     /locus_tag="VFQ61_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955614.1"
                     /translation="GPDTKDPQDRRSAAPSPGGESDAKPPLEGCTPKCGAACGTDDGC
                     GSKCGCERGLECKEGVCLDPSCGPCRPNERCNAGKCECVPDCEGRACRDDGCGGQCQC
                     PDGTVLNPQQQPVPRDQCKDTCGSAHFQCGEVCGVNCGTCGEGQACDAGVCKCAPKCD
                     GTSCSDGCGGSCDCAGGTVCNAGNSCVKPAECKDTCESTGLRCGSICGQECGSCGNGQ
                     SCVDGQCREGVSCADCGLQIRLLERQVLANKIVRTKIAVDYQATSEETGPRLVDTRIG
                     ADHVVNLVEAEPGPALIQTGKDLFVDETTHRAWQEKPDESFQLLAYSVSGTLRVKSGR
                     LVTLTFDLAEPGPVRFSLLRHAQTFAPLDADNALQSSKYEQPLVVTR"
     gene            1144..6222
                     /locus_tag="VFQ61_13970"
     CDS             1144..6222
                     /locus_tag="VFQ61_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955615.1"
                     /translation="MNLLRSTPMHGLRAVARELPRTVLALVTAALGILSGGYAHAAAL
                     DQAGLDQLHVHVDCVERCELGRLATKSSCIQACGTAPTSWNKNLDSAVNRRDYQLALV
                     EFWDAGTLTKICYESGAVVPKGRCGGSDCLATHTAAQCADADADGIKAWQEALVGTSD
                     AAAQRLCASGSECNGFSEQCSYDPVLDLSYCKARASLSAFHLEKVEENASSVVVNVVF
                     DYAPVPPTILDIYVQFNGQALVLTDSRGLLAATSAGKTVEVRQPGDNLIRVTALGISD
                     SRVIQPGPIAELVFRRKTGDATSIQFSTVQSHREDSLAPSQGGHQIGLRQDSAWGAPV
                     PLSSSADSAQGHVLVHYDFESDKRPISESSVLAPVDICPLLRLSSGRSVLAGDCPSEP
                     AVPAGGPSDPAYAAAKRKRDRWISELSVLQKGVNVTTQSVLGVTGFASYFDGRNDHLE
                     LPLTFTQPVAFGGDFTEARQNFSISLWAYQEASEVANQEQVLYTRAAQASEISQFALL
                     TRANITNDTFDLLWLSGNLSDPGAVRTVLRTGLPNRKWTHLGLSMNAATRTANVYVDG
                     VATPPVTLAVGALVTCPQIQTGFTKRMTIHEEGDFASIQGTGPETLFLASAGGNGLYG
                     IESMDPNGLGRHDVLRLTDSSAKDPDYSPIVDRIVYSSNQSGNSEIWVAWSDGTHAQQ
                     ITDGFGSTADGIFARHPRWAPDASGIVFESNARDIDARDNLEGLGYQLYFIEYDPQAN
                     AVAIPVLGSPTTKLTQLDYAERVASGDINMYRLTQVGLAASNMQPQWLAGRTGTGDLA
                     KGELAFTSTDANGSNPKPQRLRVAKTYWQGNDANKEFLPLVADPLLVKNRSVLAARAV
                     RAAGQPVLERALLAEQLSESIAAPEYQVTFNPSGTCAGGGAAFTASVKYVNPTQPEKC
                     WDVNRNQICDPSEDKNRDNACNTLDCNATEFESIYVNYNAAQAVPDMSSVQIGAWVSA
                     NEKRLRAVQAFGATTDAVRLEMTSPVNSTPIPNGTELVQIKFCGSARPTTNVQKLVIK
                     QRFFVLTSITDEVTPANNRLQVDPFTLADPRIQSIAGAEFSPDASRLALSAIFDARPT
                     LMRTKDVSGTANADMLTTEAIKVEGMSWAGTTQFYPCNWVGSVRHPTSKLYLAAFAGA
                     LDEIHLVDYVRTQAAFVSESERGHERLVKEGREEAGALGGTPCTQDATCGDGELCVSG
                     ACQIVTCQPSNTYPCARGRCQRLAVEVSPTEQYACVSECQSDATCQEQQCANGPCRFC
                     DTTARTCSECRKVVENVGGLSLEYIQGCPDRNSFACDRGTCVSECYSFRNGETKYLCD
                     PATEYCRAGRCTLFDWNWTDFSPVSFAGAGEMTSHKIKPTTAISQLYPVEIKAFGSGD
                     YAHPPELIVEAQAPGVFGGNWFDIGRVVVSNETKSEAEEKPYVLNSPYPITTLRLRNI
                     VPVYENLDNAAMGLINGRSGEFCTGANCRFAAQGSRFWLGYEASIPGHVAKCQRDPGS
                     CQPGHVRYMRPGTPTLLILQVKVKNQEQMIGTWNNKICPYWNGSAAVAEPSDTSTPPI
                     PYPMIYGDAARENSNQKRKYYPSAPASSLLTFNASSSGFGLINCNYVDDTGSAAALAG
                     IQITVEGVSYTQLTEGDTITETANSCKVNVGTPQTPRYEACFEWSGADVSFDPFASEP
                     IPYRTLSLTRFKSFGWGNPGDATP"
     gene            6263..>7591
                     /locus_tag="VFQ61_13975"
     CDS             6263..>7591
                     /locus_tag="VFQ61_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955616.1"
                     /translation="MSRFRIERSHAWKFVLGAVGCAMVGTLFLQRASAQSTPDECALA
                     GKNCSFTISDNSIQKVPTLFKLQAQIAQAKIPVGDAVFTKLVVNIKSGNTVRCTESFD
                     QVRVRSGVLNIEIGRNIQGCDLDTTVARYNDLTFQVCIGDSTSEAGNCLKPIQFSSVP
                     YAIKASFASQAQESYRSEISARANYAQRLVADGTRLDQTKLGIGYYDFETPGSGKLSL
                     LTAVNPTQSADVKGGFLQWTPVDSANKFMNFSTKNPSTGNLEPFTKLLFHAGETNVWG
                     KFSVVDTAAFQKEAVFGRGLSVLTGNVSVNADATFQRPLTATNTVTTGGMLTANGGIT
                     AANGIKVNSGTLDVASSAAFGSTSASTAVTFRAGTTVDMSAAAQVILPTNTVLVPSVK
                     TLTASSSASCVTSAPAAGTTFCAISLMTPLATGVNKGCWIGTSTSPAGRTL"
BASE COUNT         1481 a         2361 c         2412 g         1337 t
ORIGIN      
        1 cggccccgat accaaagacc cgcaggatcg ccgctcagcc gcgccctcgc cgggtggcga
       61 gagcgacgcg aagccgcctc tcgaaggctg tactccgaag tgcggcgcag cgtgcggaac
      121 ggacgacggc tgcggctcca agtgtggctg tgaacgaggg ctcgagtgca aggagggagt
      181 ctgtttggac ccctcatgcg gtccttgtag accgaacgag cgctgcaatg ctggaaagtg
      241 cgagtgcgtg cccgattgcg agggtcgcgc ctgccgagac gatggttgcg gcggacagtg
      301 ccaatgcccc gacggtacgg tgctcaatcc tcagcaacag ccggtacctc gggatcaatg
      361 caaagacacc tgtgggagtg cccacttcca gtgtggcgaa gtctgcgggg tcaactgcgg
      421 gacctgcgga gaagggcagg cttgtgacgc gggggtctgc aagtgcgcgc ccaagtgcga
      481 cggaaccagc tgttccgatg gctgcggcgg cagctgcgac tgcgccggcg gtacggtgtg
      541 caacgccggg aattcctgcg tgaaacccgc cgaatgcaag gacacgtgtg agtctacggg
      601 gctgaggtgc ggctcaatct gcggccagga gtgcggcagc tgcggcaacg ggcagtcgtg
      661 cgtcgacggg cagtgcaggg aaggcgtgag ctgcgcggat tgcggtttgc agattcggct
      721 gctggagcgc caggtgctgg cgaacaagat cgtacgcacg aagatagccg tagactatca
      781 ggcaaccagc gaagagaccg gccctcgact cgtggacacg cggatcggtg ccgatcatgt
      841 cgtgaacctc gtcgaggccg aaccaggtcc cgccctgatt caaaccggaa aagacctatt
      901 cgtcgacgag acgactcatc gcgcctggca agagaaaccg gacgagagct tccagctgct
      961 cgcgtacagt gtctcgggca ctctgcgagt caagtccggt cggctcgtca cgctgacgtt
     1021 cgacctcgcg gagccaggcc cggtgcggtt ctccctgctg cggcacgcgc agacgttcgc
     1081 gccactcgac gcggacaatg ctcttcaatc ttctaagtac gagcaacccc tcgtggtgac
     1141 ccgatgaatc ttctgcgctc gactccgatg catgggcttc gagccgtcgc acgggaactc
     1201 ccgcgcaccg tgctcgcgct cgtgaccgcg gcgctcggga tcttgagcgg cggctacgcg
     1261 cacgctgcgg cgctcgatca ggctgggctc gatcagctgc acgttcatgt cgactgcgtc
     1321 gaacgttgtg agctcggtcg gctcgcgacc aagtcctctt gtattcaggc gtgtggcaca
     1381 gcccccacgt cttggaacaa gaatctcgac agcgccgtca atcgccgcga ttaccaactt
     1441 gcgctggtgg agttctggga cgcgggcacg ctgaccaaga tttgctacga gtccggggca
     1501 gtggttccca aagggcgctg cggcgggtcg gactgcctcg cgacgcatac ggccgcgcag
     1561 tgcgccgacg cggatgcgga tggaatcaaa gcctggcaag aggcgctcgt ggggaccagc
     1621 gacgcggccg cacaacggct ctgcgcttcc ggcagcgagt gcaacggatt ctccgagcag
     1681 tgctcgtacg atccggtgct cgatctctcc tactgcaagg cccgtgcgtc gctctccgcc
     1741 ttccatctcg agaaggtcga agagaacgcg agcagcgtcg tggtcaatgt ggtgttcgac
     1801 tatgcgccag tgccgccgac gatcctggac atttatgtgc agttcaacgg ccaagccctc
     1861 gtgctcacgg attcccgagg gctgctggcg gccacgagtg ctgggaagac ggtggaggta
     1921 cggcaaccgg gcgacaacct aattcgcgtc accgcgctcg ggatcagcga ctcgcgcgtg
     1981 attcaacccg gccccatcgc cgagctcgtg ttccgccgga agacagggga cgccacgagc
     2041 atccaattct cgacggtgca gtctcaccgc gaggacagcc tcgccccgtc tcagggtggc
     2101 catcagatcg ggcttcggca ggacagcgct tggggtgcgc cggttccgct ttctagcagt
     2161 gcggattcgg cgcagggcca cgtgctggtt cactatgact tcgagtcgga caagcgcccg
     2221 atctcggagt cctcagtgct ggctccagtc gatatctgtc cgctcctacg tttgagctcg
     2281 ggtcgctcgg tgctcgctgg ggactgccca tccgaacccg cagtgcccgc gggagggccc
     2341 tcggacccgg cgtacgcggc ggcgaagcgc aagcgcgatc gctggattag tgagctctcc
     2401 gtattgcaga agggggtgaa cgtcacgacg caaagcgtcc tcggtgtaac cggtttcgcg
     2461 agctacttcg atggtcgtaa cgaccatctc gagctgccgc tgacctttac gcagccagtg
     2521 gcgttcgggg gtgatttcac ggaggctcgc cagaacttct ccatttcctt gtgggcgtat
     2581 caagaggcct ccgaagtcgc gaaccaggag caggtgcttt acacgcgcgc cgcgcaggcc
     2641 agtgaaataa gccagttcgc acttctgacg cgagcgaaca tcacgaacga cacgttcgat
     2701 ctgctgtggc tctcgggcaa cctctccgat ccgggcgcgg tgcgcacggt gctccgcact
     2761 gggctaccga accgcaagtg gacgcacctg ggattgtcga tgaatgcggc cacccgcacg
     2821 gccaacgtct acgtggacgg cgtggctacc ccgcccgtga ccctcgcggt gggcgcgctc
     2881 gtgacctgcc cacagattca gaccggcttt accaagcgca tgaccatcca cgaggagggt
     2941 gatttcgcga gcattcaagg gaccggcccc gaaacgctgt tcttggcgtc tgccggcggc
     3001 aacgggctct acggcatcga gtcgatggac ccgaatggcc tcgggcgtca cgacgtgctc
     3061 cgcctcaccg acagctccgc gaaggatccc gactacagcc caatcgtcga tcgcatcgtc
     3121 tattccagca atcagagcgg gaacagcgag atttgggtgg cttggtccga tggcactcac
     3181 gcgcagcaga tcacggacgg cttcggtagt accgcggacg gcatattcgc gcggcatccg
     3241 cgttgggcac cggacgcctc cggcatcgtg ttcgagtcga acgcgcgcga catcgacgct
     3301 cgagataacc tggaggggct aggctatcag ctctacttca tcgagtatga tccgcaggca
     3361 aacgcggtcg cgatcccggt cctggggagc ccaaccacga agctcactca gctcgactac
     3421 gccgagcgcg tcgcgagcgg cgacatcaat atgtatcgcc tcacgcaggt gggcttggcc
     3481 gcgagcaaca tgcagcccca gtggctcgcg gggcgcaccg gtacgggcga cctagccaag
     3541 ggagagctgg cttttacgtc aaccgacgca aacggctcga atccaaagcc ccaacgcttg
     3601 cgcgtggcca agacctactg gcaaggtaac gacgcaaaca aggagttcct gccgctcgtg
     3661 gcggacccgt tgttggtcaa gaatcgcagc gtgctcgcgg cgcgcgcggt acgtgcggcg
     3721 gggcagcccg tactcgaacg tgccttactc gccgagcagc tctcggagag catcgccgcg
     3781 ccggagtatc aagtcacgtt caacccgagt gggacctgcg ccgggggcgg cgcggcgttt
     3841 acggcctctg tcaaatacgt gaatcccacc cagcccgaga agtgttggga cgtgaaccgc
     3901 aatcagatct gtgatccgtc ggaggacaag aaccgagata acgcctgcaa tacactcgac
     3961 tgcaatgcga ccgagttcga gtccatctat gtgaattaca acgccgccca ggccgttccg
     4021 gacatgtcgt ccgtgcagat cggggcgtgg gtcagcgcga acgagaagcg actgcgagcg
     4081 gtacaagcat tcggtgcgac gacggacgcc gtgcggctcg agatgacgag cccggtcaac
     4141 agtacgccca tcccgaacgg caccgagctc gtgcagatca agttctgcgg ttcagcgagg
     4201 ccgaccacca acgtccagaa gctcgtcatc aagcagcgct tcttcgttct cacgtcgatc
     4261 acggatgaag tcacgccggc aaacaaccgg ctgcaggtgg acccgttcac gctcgccgat
     4321 ccgcgcattc agagcatcgc gggcgccgag ttctcgccgg atgcgagtcg gctcgcgctg
     4381 tccgcgatct tcgatgcacg gccgacgctg atgcgcacca aggacgtcag cggcaccgca
     4441 aacgccgata tgctgacgac ggaggctatc aaagtcgagg gtatgagctg ggcgggtacg
     4501 actcagtttt acccctgcaa ctgggtgggc agcgtacgcc acccgacgag caagctgtat
     4561 cttgccgctt tcgcgggcgc actcgacgag attcacctcg tcgactacgt acgaacccag
     4621 gctgccttcg tttcggagtc cgagcgtgga cacgaacgac tggtcaaaga agggcgcgaa
     4681 gaggccggcg cgctgggtgg caccccatgc acgcaggacg cgacctgcgg cgatggcgaa
     4741 ctttgcgtct cgggcgcctg ccagatcgtc acctgtcagc ccagcaacac gtatccctgt
     4801 gcgcgcggcc gctgccagcg gctcgcggtc gaggtctccc cgactgaaca gtacgcctgt
     4861 gtgtcggagt gccaatcgga cgctacgtgc caggaacagc agtgcgcgaa cggcccgtgt
     4921 cgcttctgcg acacgaccgc acggacctgc agcgaatgcc gtaaagtcgt ggagaacgta
     4981 ggcgggctga gcctggagta catccagggt tgccccgatc ggaatagctt tgcatgcgac
     5041 cgcggaacct gcgtcagcga gtgctattcg ttccgtaacg gggagacgaa gtacctgtgc
     5101 gatccggcga ccgagtattg ccgcgccggg cgctgcaccc tgttcgattg gaactggacg
     5161 gacttctcgc cggtgtcctt cgcgggcgcg ggtgagatga cgagccacaa gatcaagcct
     5221 accaccgcga tctcgcagct ctatcccgtg gagatcaagg ccttcggatc gggcgactac
     5281 gcccaccccc cggagctcat cgtagaagct caggcgccgg gcgtgttcgg tggcaactgg
     5341 ttcgacatcg ggcgtgtcgt cgtcagtaac gagacgaagt ccgaagctga agagaaaccg
     5401 tatgtcttga attcaccgta tcccatcacg acgctgcgat tacgcaatat cgtgcccgtg
     5461 tacgagaact tggacaacgc ggccatgggc ctgatcaacg gtcggtccgg ggagttttgc
     5521 acgggagcaa actgccgctt cgccgcccag ggcagccgct tctggctggg atacgaggcc
     5581 tcgattccgg gtcacgtcgc gaagtgccaa cgcgatccag ggagctgcca gccggggcat
     5641 gtccgctaca tgaggccggg aacaccgact ctgctcatcc tccaggtgaa ggtgaagaat
     5701 caggagcaga tgatcgggac gtggaacaac aagatttgtc cgtactggaa tggaagcgcc
     5761 gcggtggcgg agccgtcgga cacgtccact ccgccgattc cgtacccgat gatctacggc
     5821 gacgcggcgc gagagaattc caaccagaaa cgcaagtact atcccagcgc gcccgcatcg
     5881 tcgctcttga cgttcaacgc gagcagcagt gggttcgggc tcatcaactg caactatgtc
     5941 gacgataccg gatctgccgc tgcgctcgcg ggtatccaga ttaccgtcga gggcgtttcg
     6001 tacacccagc tcacggaagg ggacacgatc accgagacgg cgaacagctg caaggtgaac
     6061 gtcggtactc cgcaaacgcc gcgctacgag gcgtgtttcg aatggtctgg cgcggacgtg
     6121 agcttcgacc cctttgcgag cgaacccatc ccgtaccgca cgctgtctct tacccgattc
     6181 aagagcttcg gatggggcaa ccccggagac gccaccccgt gagcgcgaga aaaactcaat
     6241 tggaacaagg aaggattaaa agatgagtcg ctttcgaatc gagcgctccc atgcctggaa
     6301 gttcgttctc ggggcggtgg gctgcgcaat ggttgggacc ctgtttctcc agcgcgcgtc
     6361 agctcagtcg acgccggacg agtgcgcgct cgcggggaag aattgcagct tcacgatctc
     6421 cgacaactcc attcaaaagg tgccgaccct attcaagctt caggcacaga tcgcccaggc
     6481 gaagattccc gtgggcgacg ccgtgttcac gaagctggtc gtcaacatca agagcggcaa
     6541 caccgtgcgt tgcacggagt cgttcgacca agtgcgcgtt cggagcggcg tattgaacat
     6601 cgagatcggt agaaacatac agggttgcga tctcgatacg accgttgctc gttacaatga
     6661 cttgaccttc caggtctgca ttggggactc gacatcagag gccgggaatt gcctgaagcc
     6721 gatccagttc agcagcgttc cctatgcaat caaggcgagc ttcgcgagtc aagcccagga
     6781 gtcttatcgc tcggagatct ccgcgcgggc caactacgcc caacgtctgg tggcagacgg
     6841 gacccggctc gatcaaacga agctcggcat aggctactac gacttcgaga cccccggatc
     6901 cggcaagctc tctctgctca cggccgtaaa ccccacgcag tcagcggacg tgaagggtgg
     6961 ctttttgcag tggactccgg tggattctgc gaacaagttc atgaactttt cgacgaagaa
     7021 ccccagcacc gggaacctgg aaccgttcac gaagctgctt ttccacgcgg gcgagaccaa
     7081 cgtctgggga aagttttcgg tcgtcgatac cgcggccttc cagaaggaag cggtgttcgg
     7141 gcgcgggctc agcgtgctca ccggcaatgt ctccgtgaac gcggacgcta cattccagag
     7201 accgctcacc gcgaccaata ccgtcaccac cggcggcatg ctgaccgcga acggcgggat
     7261 taccgcggcc aatgggatca aggtcaactc gggcacgctg gacgtcgctt cgtccgcggc
     7321 gttcggtagc accagcgcga gtaccgcggt gacctttcga gcgggaacca cggtggacat
     7381 gtccgccgcc gctcaggtca ttctgccgac caataccgtg ctcgttccaa gcgtgaagac
     7441 tttgacggcg agttcatccg cgtcttgcgt tacgagcgct cccgccgccg gcacgacgtt
     7501 ctgcgcgatc tcgctgatga ctccgctcgc gaccggcgtg aacaagggtt gttggattgg
     7561 aacctcgacc tcgcctgctg gtcgcacgtt g
//
LOCUS       DASTKU010000407         5367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1191272, whole genome shotgun sequence.
ACCESSION   DASTKU010000407 DASTKU010000000
VERSION     DASTKU010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191272"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VFQ61_13980"
     CDS             <1..1155
                     /locus_tag="VFQ61_13980"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9955617.1"
                     /translation="AGCSVILTAFRWVLSEWCSDGALGDPPNQRPAAGERAEKIMKAF
                     VTGSTGLLGNNLVRTLLAEGHEVLALARSREKAQRELGDTDAQIVIGDLTETRGFSEA
                     LRGAHVVYHTAAYFREYYGPGDHAALYRTNVEATLELARAALAAGVPRLIHTSSAAIV
                     GNQPDGSPGDESTPPSALVRDNAYVQSKIALEPLLEAFAREQGLFVASVLPGWMWGPH
                     DAGPTPSGQLVRDALRGKLPPAVPPGGAEVVDARDVAQAMIRIAARGRAGERYLVSQG
                     FLSLLEIVKLLGAVTGRSAPSATLPYPVALAFASVAEAWAKLTGTASAISLPAIRMMH
                     ARLCFSNYKFRTQLGVTPRPFEETLRDTVEWTVRRLHSGSTERTVTGGPA"
     gene            complement(1175..1606)
                     /locus_tag="VFQ61_13985"
     CDS             complement(1175..1606)
                     /locus_tag="VFQ61_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955618.1"
                     /translation="MLNTASVCVGRLVEIRVGAGYRSSADVDAVFAQIARAISTRPNQ
                     IWVAVADWRYCPIMSSDAAERALAGMTRNNPQVERSAALASAHSPTAVLQFTRLVRES
                     NNERRRLFDDASALIAWIGEVLTRPELTRLEEFLREAPPRR"
     gene            complement(1661..2911)
                     /locus_tag="VFQ61_13990"
     CDS             complement(1661..2911)
                     /locus_tag="VFQ61_13990"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9955619.1"
                     /translation="MPNVERSVWSRILRAGRSSPSIAAIALLLAGCSTPDEPSSEGIE
                     LGLLLPFTGADSATSSNFERAVLFALRGVNAGGGIGGRPLRVVSADTHSDPARARSSL
                     DQLVDAGVSVVIGPESADIAAQIAPELASRGVAFLSPLVGAAEDANVDCSVPWFRLAP
                     SAKTLGDALAKQLSAEAIQRVAILYEPRAYEQAMRSAVGNRFKALGGTVALEQELDPQ
                     AQGYGSAVLSAIQADVQAVVLATSPRTAALVVNDYRASSPVLPRWFLSPLLKTELLLA
                     NVPPEVLEGAIGVAPKIYERSREFVAEFAHYWEGDEPLEGAYFYYDAVGLLALALQRT
                     ALNHDGQIELKNLDSAVLQTATTRGVSVGWNEIASGLARVADGGNTYYSGLTGPLLLD
                     SCGPRTLGASTVWRIEGGVIVESE"
     gene            complement(2946..4214)
                     /locus_tag="VFQ61_13995"
     CDS             complement(2946..4214)
                     /locus_tag="VFQ61_13995"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9955620.1"
                     /translation="MKLERILKFASTCVAVWLGAVACSNADEQRPNTGDGGATGTAQR
                     SVEVFSWWIAPGEAEAFQSLVDLNKQLHPQTRVFNAALDSGDNARAELARRLDANDPP
                     DLFQQNAHDIAAFLTRRPGTLQPLDDLLAATGLDTAIQADVLKDVTFGGHVYGLPVNI
                     HRENALFYNKQLFKEAGIAVPTSTPELLAACETFKAAGVTPIATAHQGWILRILFNSL
                     AMGYMGSNAFNAFMSGGERDDVALRGAIDLFDDVLTKYVNDDAEEADFGWTDAAELVA
                     NGEAAMFFHGDWAKGFYGQLGWTAGVDFGVVGAPGASDMFWYGVDVFSLPTGAQNAPS
                     AQEFLTTIGSVQGQLAFNRIKGSTPVRLDVPKAQLDIEGRATLDDFANATYRTFVVSL
                     DAWDAAMLAFAEDHDKDALFQAYVDHPPQH"
     gene            4661..4888
                     /locus_tag="VFQ61_14000"
     CDS             4661..4888
                     /locus_tag="VFQ61_14000"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HET9955621.1"
                     /translation="MDPNTDQLRSQFEEFDLNGDGLLDSEEFHDLLGSLGVSANPDLI
                     EIAFGAIDLNSDGRIDFTEFCGWWKSRSEDG"
     gene            complement(4981..>5367)
                     /locus_tag="VFQ61_14005"
     CDS             complement(4981..>5367)
                     /locus_tag="VFQ61_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemopexin repeat-containing protein"
                     /protein_id="HET9955622.1"
                     /translation="QYTRLDKAGMKLDYAPRELPGGWNNWPAHWNSVTAAIKWTDGKL
                     YLFRGNEFVRLTGTTVDNGYPQPISNKFNIPYTSGFDYAFIGSNGKGYFFKGPDYVRV
                     TITNHQASVDANYPRPIVGRWPGVTF"
BASE COUNT         1015 a         1674 c         1675 g         1003 t
ORIGIN      
        1 gccggttgct ccgtcatctt gacggcgttc agatgggtcc tatctgagtg gtgttcagat
       61 ggcgcgctcg gcgacccgcc aaatcaacgg cccgcggcgg gtgagcgcgc ggagaagatc
      121 atgaaagcat tcgttactgg ctccaccggc ttactcggca acaatctcgt gcgcacgctg
      181 ctcgcggagg ggcacgaagt actagcgctg gcccgctcgc gagagaaggc gcaacgtgag
      241 ctcggcgata ccgacgcgca aatcgtgatc ggggacctga ccgaaacgcg agggttctcc
      301 gaggccttgc gcggcgctca cgtcgtttac cacacggccg cgtatttcag agagtactac
      361 ggcccgggcg accacgccgc gttgtatcga accaacgtcg aagcaacgct cgagttggct
      421 cgcgcagccc tcgccgcggg cgtaccaagg ttgatccaca cgagctccgc cgccatcgtc
      481 ggcaatcagc cagacgggag cccgggcgac gagagtacgc cgccatccgc actcgtgcgc
      541 gacaacgctt acgttcagag taagatcgcg ctcgaacctt tgctcgaggc gttcgcgcgc
      601 gagcagggct tgttcgttgc ctccgttttg cccggttgga tgtggggacc gcacgacgcg
      661 gggcccaccc cgagtggcca actcgttcgg gatgctctgc gcggcaagct tccgccggca
      721 gtgccgcccg gtggcgccga ggtcgtggac gcgcgtgacg tagcgcaagc aatgatccgt
      781 attgcagctc gggggagggc cggggaacgc tacctcgtga gccaggggtt tctctcgctg
      841 ctcgagatcg tgaagctgtt gggtgctgtc accgggcgct ccgcgccgag cgctaccctt
      901 ccgtatccgg ttgctctcgc cttcgccagc gttgcggagg catgggccaa acttacgggt
      961 acggcaagtg cgatttcgct gccggccatt cgcatgatgc acgcccgctt gtgcttctcg
     1021 aactacaagt ttcgcacgca actcggggtg actccgcgac ccttcgaaga gaccttgcgt
     1081 gatacggtcg agtggacggt acgaaggctg cactcgggct cgacggagcg aaccgtgacc
     1141 ggtgggcccg cttgacgaga ctacttggtt gcggctagcg ccgaggcgga gcttcgcgca
     1201 ggaactcttc caagcgggtg agctcgggac gagtcagcac ctcaccgatc caagcgatga
     1261 gcgcgctcgc gtcatcgaac aagcggcggc gctcattgtt gctctcccga accaagcgcg
     1321 tgaactgtag aaccgcggta ggagagtgtg ccgaagcgag cgccgcgctt ctctcgacct
     1381 gaggattgtt tctcgtcatg cccgcgaggg cccgctcggc cgcgtcactc gacatgatcg
     1441 ggcaatagcg ccagtcagcg acggcgaccc agatctggtt cggtcgagtc gagattgccc
     1501 gcgcgatttg cgcgaacacg gcatcgacgt cggcgctgga gcggtaacca gcacccacgc
     1561 gtatttccac caagcgaccg acgcacacgc tggcggtatt cagcatatcg tcttcaacgg
     1621 cgaacgtagg cgtcaataaa ggccactgag ggcgctggat tcactcggat tcgacgatga
     1681 ctccaccctc gatgcgccag accgtcgaag cgcctagcgt cctcggacca cagctatcga
     1741 gcaggagtgg gccagtcagt cccgaatagt aggtattgcc tccgtcggca acccgagcca
     1801 gcccagacgc aatctcgttc caaccgacgg agactccacg ggtagtcgct gtttgcagta
     1861 cggcactatc caggttcttc aattcgattt gcccgtcgtg attgagggcg gtccgctgga
     1921 gagccagcgc cagcagaccc accgcatcgt agtagaaata agcgccttcc agcggctcgt
     1981 cgccctccca gtagtgagcg aactcggcga cgaactcgcg ggaccgctcg tagatcttgg
     2041 gggctacccc gattgcgccc tcgagcacct caggggggac attcgccaac agcaactcgg
     2101 tcttcagcag cggagatagg aaccagcgcg gcaagaccgg gctcgaagcg cggtaatcat
     2161 tcactaccaa cgcggcggtt cgcggtgagg tagcgagcac cacggcctga acatctgcct
     2221 gaatcgcgct gagcaccgcg ctgccatagc cttgcgcctg gggatcgagt tcctgctcga
     2281 gcgccactgt gccgcccaag gccttgaagc gattgcccac cgcgctgcgc atcgcctgct
     2341 cgtacgcgcg tggctcgtaa aggatagcca cacgttggat agcctcggcg gacagctgct
     2401 tggcgagcgc gtcccccaag gttttcgccg acggcgccaa gcgaaaccag gggacgctgc
     2461 agtccacgtt cgcatcttcc gcggcgccca cgagcggcga caggaacgcg acgccgcgag
     2521 acgctagctc gggcgcgatt tgcgcggcaa tatctgcgct ctccgggccg atgaccacgg
     2581 acacgcccgc gtctacgagt tgatcgagag aactgcgcgc gcgtgcgggg tccgaatgcg
     2641 tatctgcgga gacgacgcgc agcggtcgtc cgccgatccc gccgcccgca ttgacccctc
     2701 tcagcgcgaa tagaaccgca cgctcgaagt tgctcgaagt agccgaatcc gcacccgtga
     2761 acggcagcag tagacccagt tcgatcccct cactcgatgg ctcgtccggg gtagagcaac
     2821 cggcgagaag caaggcaatc gccgcaatgg acgggctcga ccgacccgcg cgcagaatcc
     2881 tgctccaaac ggaacgctcg acattcggca tcttcggcac ccttacgccc aaagctggtg
     2941 tggcgctagt gctgcggtgg gtgatccaca taggcttgaa acagggcatc tttatcgtgg
     3001 tcttccgcga acgccagcat ggcggcgtcc caggcatcca gactcaccac gaaggttcgg
     3061 tacgtggcat tggcgaagtc gtccagagtg gctcgccctt cgatatccag ctgagccttc
     3121 gggacgtcca gtcgcaccgg cgtggagcct ttgatcctat tgaatgcaag ctggccttgc
     3181 acgctcccga tggtggtcag gaactcttgc gcgctcggcg cgttttgggc gccagtcggc
     3241 agtgaaaaca cgtctacgcc ataccagaac atatcggagg ctccgggcgc acccacgacc
     3301 ccgaaatcca cacccgcagt ccagccgagt tgcccgtaga agcccttggc ccaatcgccg
     3361 tgaaagaaca tcgcggcctc gccattcgcg accagctctg ccgcgtcggt ccagccgaaa
     3421 tcagcctcct ccgcgtcgtc gttcacgtat ttcgtgagta cgtcgtcgaa cagatcgatc
     3481 gcgccgcgca aagcaacgtc atcccgctca ccccccgaca tgaacgcatt gaaagcgttg
     3541 gaccccatgt agcccatggc caaagagttg aatagaatcc gcagaatcca gccttgatga
     3601 gcggtggcga tgggcgtcac gccagccgcc ttgaaggttt cgcacgcggc caacaactcc
     3661 ggcgtgctcg tcgggaccgc aatccccgct tctttgaaca gctgcttgtt gtaaaatagc
     3721 gcattctctc ggtggatgtt gacgggcaag ccgtacacat ggcccccgaa cgtgacgtct
     3781 ttcagcacgt ccgcttgaat tgcggtatcg agcccggtcg cggcgagcag gtcgtccaga
     3841 ggctggagcg tcccggggcg tcgggtcagg aaggcggcaa tatcatgcgc gttctgctgg
     3901 aagaggtctg gcggatcgtt cgcatccagg cgtcgggcca gctcggctcg ggcgttgtca
     3961 ccggagtcta gcgcggcgtt gaaaacgcgg gtctgaggat gaagttgctt gtttaggtcc
     4021 actagcgatt gaaaggcctc ggcctctccc ggagcgatcc accaggaaaa cacctctacg
     4081 ctgcgttgcg cggtgccggt cgcacctccg tcccccgtgt tcgggcgttg ctcgtctgcg
     4141 ttgctacacg cgaccgcgcc caaccagacg gctacgcacg ttgaggcaaa cttgaggatc
     4201 cgttctaatt tcatccgtgt gtgacctcga cggcgagttg gctcgcgtag atacgaccac
     4261 gccaggcgtg aaacttagat cggcgctgaa aggcacgata agccgccgca cgagacccta
     4321 gaacgactca cttcctcggg gcgattttgg gggccttggc gagaaccgat cgcagcgctg
     4381 gatcctgcag gttggaaacg agcgtctgat gtgggctagg atgcgcctga gcggccccgt
     4441 actgctccgc cggcgcggtc atgagcaagc cgaatcgagg acgtggtcat cgcgctgaaa
     4501 gcgaggttgg ggagctgctc gttgcggaag gatcgtccac gctcgtcgag ggtacggtcg
     4561 tccatgcggt caggcgccgc gagtgattcg ctgaaaccgc tgcggaaacc gtaagcgcag
     4621 gtgaccctgg agcgcgcgag ctttctgcta tacctggcgc gtggatccga atacagacca
     4681 acttcgtagc caattcgagg agttcgacct caacggggat ggcctgctgg attcggaaga
     4741 atttcacgat ctgctgggct cgctcggcgt ctcggcgaac ccagacctga tcgagatcgc
     4801 gtttggtgcc atcgacctga acagcgacgg gcggatcgac ttcactgagt tttgcggttg
     4861 gtggaaaagt cgaagcgagg acggatgatt cgggcccggc tcagcatcgc ctcaccgaaa
     4921 ctgaatcggt tacacctggt gacccggctt tgacggggcg actcgtcgga tgagtctatt
     4981 tcagaaagtg actcccggcc aacgtcctac gattggcctc gggtagtttg cgtcgacgct
     5041 tgcctggtga ttcgtgatgg tcactcggac gtagtcgggc cctttgaaga agtagccctt
     5101 tccgttgctg ccaatgaagg cgtaatcgaa gcccgacgtg tacggaatgt tgaatttgtt
     5161 ggaaatcggc tgcggatagc cgttgtcgac cgtggttccg gtcaggcgaa cgaactcgtt
     5221 ccctctgaag agatacaact taccgtcggt ccacttgatc gccgccgtga ctgagttcca
     5281 atgcgcgggc caattgttcc agccccccgg cagctcccgc ggagcgtagt cgagcttcat
     5341 tccagccttg tctagtcgcg tatattg
//
LOCUS       DASTKU010000408         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_522560, whole genome shotgun sequence.
ACCESSION   DASTKU010000408 DASTKU010000000
VERSION     DASTKU010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522560"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VFQ61_14010"
     CDS             <1..542
                     /locus_tag="VFQ61_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955623.1"
                     /translation="EYAALGGIVAGPEALRRFAGAATRNTDDHPVVAYSAPRITYAPV
                     SSPRERLMTLLQALEAHPDELLMAAPERDADRLIAYWNARNRFIELGRNVRPAPDVES
                     MLRRVRDPLLSVLRISRDFRPAYDPLLSMAVALSRSDAESASWLLAELQTIQPARREA
                     SRALAELAARPRRLDVYEH"
     gene            730..>2203
                     /locus_tag="VFQ61_14015"
     CDS             730..>2203
                     /locus_tag="VFQ61_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955624.1"
                     /translation="MRTVNKALLLALGFGALGTVAGCGERNTPSETNSDAVGSASFEV
                     RLGDGRAIDSARYTITGPKDFSKTGSINVSASNTLSATIPGLPAGGGYEIQVTAKTRE
                     GSVDCSGVAKFDVIARKTVNVVLALSCHEAPRLGSVSITGTLNLCPTIDGIGADPAEV
                     QVEATIALSSLAHDSDAGPKPLTYAWRATSGTFSDPAAPNPTFTCTKPGPVTLTLSVS
                     DGDPETSCADQLSTQITCSAGAKAPGSYAAGDFHNHSTCSDGAISMQKLVQKATGSGD
                     GSWGLDWFVQAGHGGNGNRNCTLVEDATLDTPAYPYVSSKSPTTSWEDSGISPKGDSS
                     GTSPKRNMWRWQSVQEFQYPLIEYLNAYKNLPLFLGLETVVPGHEHTSMSVITGQIPT
                     GLDSTKLPTTPGYTALGNANALANWEYCFDRGDADKSRGAENAWDCSVPGSANESDAS
                     WNVNAKKLLPAGGVGSGTRGHAKTLESLKWLAAYHPNTSYY"
BASE COUNT          446 a          726 c          660 g          371 t
ORIGIN      
        1 acgaatacgc cgccctgggt gggatagtgg caggccctga agcgctgcga cggtttgcag
       61 gcgcggccac gaggaacacc gatgatcacc cggtcgttgc ctactcggcc ccacgcatca
      121 cttacgcacc cgtatccagc ccacgcgagc gcctgatgac gctgctccaa gcgctcgaag
      181 cccacccgga cgagctcctg atggccgccc ccgagcgtga cgcggaccgg ctcatcgcgt
      241 attggaatgc gcgtaatcgc ttcatcgagt tgggccgcaa tgtccggccc gcgccagacg
      301 tggaatccat gctgcggaga gtgagagacc cactactttc cgtgctacgc atcagccgcg
      361 atttccgtcc cgcctacgat ccgctgctgt ccatggcggt ggcgctctct cggtcggatg
      421 cggagtcggc aagctggttg ttggccgaac tccaaacgat tcagcccgcg cggcgtgagg
      481 cgtcccgcgc gctagccgaa ttggcagctc gcccgcgccg actcgatgtt tacgaacatt
      541 gaccagtcct ttgcggcacg tcactccgtc ctcgaccgat tgcgccagcg gcggcaattc
      601 cgccaactct gattgtatcg ggtttgtcac ctgatttacc cgcggccgtc tcgcaactct
      661 ggtggattac gccggcaaac tgactacgcc gacaacctcg cgtgggtgcg tgtggagcgg
      721 agacgaagca tgagaaccgt aaacaaggca ctgttgttag cattgggatt cggggcgttg
      781 ggcaccgtcg cgggttgcgg cgagcgcaac acacctagcg aaacgaactc ggatgcggtg
      841 gggagcgcca gcttcgaggt tcgcctcggg gatggtcgag ccatcgatag tgctcgttac
      901 accatcaccg gccccaaaga cttctcgaag acaggctcaa tcaacgtctc cgcgtccaac
      961 actctcagcg ccacgatccc tgggcttccc gccgggggag gctatgaaat ccaagtcacg
     1021 gccaagacgc gcgagggctc agtggactgc tccggcgtcg ccaaattcga cgtgatcgcg
     1081 cgtaaaacag tgaacgtcgt gctggccctg agctgccacg aagcgccgcg cctgggctcg
     1141 gtttcaatca cgggaaccct caacctgtgt cccacgatcg acggcatcgg cgctgatcca
     1201 gcggaggtcc aggtggaggc gacgattgcc ctatcatctc ttgcgcacga cagtgatgcg
     1261 ggccccaagc cactcacgta tgcgtggcgc gccaccagcg gcaccttcag tgatccagcg
     1321 gcgcccaacc ccacgttcac ttgcacgaag ccaggaccgg ttacgttgac cctatccgtc
     1381 agcgacggcg atcccgagac ctcctgcgca gaccagctca gcacccagat tacctgcagc
     1441 gcgggcgcaa aagcccccgg cagctacgcg gcgggggact tccacaacca cagcacgtgc
     1501 tccgatggcg ccatttcgat gcagaagttg gtccagaagg cgaccggcag cggggatgga
     1561 tcgtggggcc tcgactggtt cgtacaagcg ggccacggcg gcaatggcaa ccgcaattgc
     1621 acgctggtcg aggacgcgac cttggacacg cccgcctacc cgtacgtcag cagcaagtcg
     1681 cccacgacga gctgggagga ctctggaatt agccccaagg gcgattcgtc cggtacgagc
     1741 cccaaacgca acatgtggcg ctggcaatcg gtacaggagt ttcaataccc gctcatcgag
     1801 tacctgaacg cttacaagaa tctaccgctg ttcctcggtc tcgagaccgt agttccggga
     1861 cacgaacaca catcgatgtc cgtaatcaca ggccaaatcc caaccggctt ggacagcacc
     1921 aagctgccaa ccactccggg atacacggcc ctcggcaacg ccaacgcgct cgccaattgg
     1981 gagtactgct tcgatcgcgg agacgccgac aagagccgtg gcgccgagaa cgcttgggat
     2041 tgttcggtgc cgggcagcgc gaacgagtcc gacgcgagct ggaacgtgaa tgccaagaag
     2101 ctccttcccg ccggcggcgt aggcagcggc acacgaggac acgcgaagac tctcgagtca
     2161 ctgaagtggc tggccgctta ccatccgaac accagctact atg
//
LOCUS       DASTKU010000409        12755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_298892, whole genome shotgun sequence.
ACCESSION   DASTKU010000409 DASTKU010000000
VERSION     DASTKU010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12755
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298892"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFQ61_14020"
     CDS             complement(<1..375)
                     /locus_tag="VFQ61_14020"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HET9955625.1"
                     /translation="MSPKKTAITTEKDESSATANQPLESASRGNGGETQQYQEAGNPT
                     LTTNQGVAIADNQNSLKAFPRGPTLLEDFILREKITHFDHERIPERVVHARGAGAYGY
                     FQPYDDRLAEYTVAKFLTDPAER"
     gene            complement(679..837)
                     /locus_tag="VFQ61_14025"
     CDS             complement(679..837)
                     /locus_tag="VFQ61_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955626.1"
                     /translation="MRGLLVGGSVGYDLWTLRTLGAGIGVRADYLPFAEMSGHAASIA
                     AFAGLTYY"
     gene            906..1172
                     /locus_tag="VFQ61_14030"
     CDS             906..1172
                     /locus_tag="VFQ61_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955627.1"
                     /translation="MGFLFSDIGYWRLGNCGAFVRSVGGSTAVGSRGVAQALLARLHL
                     SPGVVREANQTFEFYGLLSSWGGRELAQPSVAQTVAKEANAHRN"
     gene            complement(1755..2303)
                     /locus_tag="VFQ61_14035"
     CDS             complement(1755..2303)
                     /locus_tag="VFQ61_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9955628.1"
                     /translation="MSLDRPATDDEICVGNVMTHPVISLDVDASVHEALMLAKQRKIH
                     HFPLTFAGRLAGFVCTCDLERASLNDSLRSCMHREVLTTAPETSTRAAAQIMNEKRVG
                     SLLVTQGDRVLGIVTREDLSKDSSSGMTIEKTRCCCCQRHHRLRPTSDGRYVCEECAR
                     DRRHAAPSEHRFGGFDENGLGD"
     gene            2590..3291
                     /locus_tag="VFQ61_14040"
     CDS             2590..3291
                     /locus_tag="VFQ61_14040"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9955629.1"
                     /translation="MGAQWKQKGREQSADAKGRLFTKLSKEIMVAARAGGPDPAVNPR
                     LRLAIEQAKRASMTRDTLERAIKKGAGLLDATTQYETVVYEGFAPHQVPVIVECLTDN
                     KNRTAPNMRQLFRKGQLGSSGSVSWDFNRRGAIEASPPAGGQDPEEAAINAGAQEVEA
                     GEEGQSRFITDATDLDAVSKALTASGWNVTSANLVWIAKNPVKLEGAARSEVEAFLAA
                     IDDDDDVQHLYVGLA"
     gene            3336..3986
                     /locus_tag="VFQ61_14045"
     CDS             3336..3986
                     /locus_tag="VFQ61_14045"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HET9955630.1"
                     /translation="MLKLVGANLNYSSWTIRPWLALTHWGIEFGFHDVGLKTRPDWRE
                     RILSFSGAGKVPILIDGALSIHESLAICEYLSELHPAAKLWPGDPQLRARGRAISCEM
                     LSSFQALRSAMNCNLRGRAKQTPRSAAIDADVRRVLEIWDASLQSSGGPFLFGEFSIA
                     DCMYFPVATRFRTYGVELPEFARGYSRALFELPAVQKLEQIASAAVPIPEYDVALG"
     gene            4013..4552
                     /locus_tag="VFQ61_14050"
     CDS             4013..4552
                     /locus_tag="VFQ61_14050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9955631.1"
                     /translation="MTAKYVFMAGIGNSEPDHWQSLWARSTPGSVWVTPQSWSEPQRD
                     DWVAALVGAVRRVGESAVIVAHSLGCLATAEASADLRQLGVRGVFLVAIPDPASPRFP
                     SCARGFKPAVACHMAVPALVVASTTDPYAGLEYAEQTAQHWNSRFVSVGDKGHINLAS
                     NLGEWEEGRRLLNDFVATL"
     gene            complement(4561..5688)
                     /locus_tag="VFQ61_14055"
     CDS             complement(4561..5688)
                     /locus_tag="VFQ61_14055"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9955632.1"
                     /translation="MVLPSMNHRLKLQWGIFIGLVIFGVVFKCSSQGTVQEIDPSAPR
                     VAGVIAISTDANGVPVAPFQLDQPPPVLELTPEQQQLLRQYNSVRRNAHGGTKYKLAC
                     SYSRSGDVGAAIYWLQRAAKEDGVDPGWAARDPDLALVRADKRGPTLFSYLDRMAEYY
                     EHHPLDRQILSLPKSYRVGRPIPMLIGLHGFGSVPEDFSGDFELMADALGMALLAVSA
                     TITRGPESFAWREDVLADTSRIERAIAAVSEQVTVTSGQIYLIGFSQGGQLALEIAAR
                     QPERFRGAIAMSPGTVSSMERIAFADSKLSGRRFVIVVGQKEHARVLATAQNSNERLL
                     QFGAQVYYHVYPGVSMHAFPPDYSEVLPKWLSFLAGGEPPT"
     gene            6011..6379
                     /locus_tag="VFQ61_14060"
     CDS             6011..6379
                     /locus_tag="VFQ61_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET9955633.1"
                     /translation="MLEPCRVCGGDGRVGNAFGGSATTCPGCRGTGRRSNTESLFHDV
                     TKTKPSHHGPATKPGAPAKITAPTTIEGVSLGNEVKASGLSEDTKAKLIREIIEYEVS
                     HGKCTQTFVKKVRKQIRPAT"
     gene            complement(6425..8140)
                     /locus_tag="VFQ61_14065"
     CDS             complement(6425..8140)
                     /locus_tag="VFQ61_14065"
                     /inference="COORDINATES: protein motif:HMM:NF024847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HET9955634.1"
                     /translation="MTSILLISGLILAIVGIATVIVIILASERAKAVAQRDGEANARQ
                     AAESRAAVAEQEVSLLSRYRQVVDAEREAAEIRATAQAEHQRAREQVQSLLFHANRQA
                     TEAVQSSEQQAQSILAQAQAQAIGTLGASHKEAQQLLGDAKARAEATLQAAEQRHATI
                     LSEADRRAQEVAGEALRAQASLEKNRREARALQNVIEGYGDQYIVPAHSVLDDLAAEF
                     GFAEAGANLKLARERTRQLLKEGRAATCDYVEANRRETAVNFISDAFNGKVDSVLSRV
                     KEDNVGTLRQKILDAFALVNMNGQAFRNARITDLYLASRLDELRWAAAAQELKNRERE
                     EQRRIKERIREEERARKDYERAMREAEKEEEAIKKAMEKIQQSVAKASNEQRQRYEAQ
                     LAELQQKLTAAEEKNQRALSMAQQTKTGHVYIISNVGSFGEEVFKIGLTRRLEPLDRI
                     RELGDASVPFEFDVHAMISSEDAPALEHALHRHFLTSQMNKVNPRKEFFRLPLATIRS
                     EVEKLGIQASWTMTAAATEYKQSIAIDKLIRENPAQLQAWIGKQLVLDAQLGADEERE
                     LAEAS"
     gene            8883..9638
                     /locus_tag="VFQ61_14070"
     CDS             8883..9638
                     /locus_tag="VFQ61_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955635.1"
                     /translation="MPVALLAGLWGLVAGAALVLGALIGWFVDVPRRVVAAIMAFGSG
                     VLISALSFDLMDEAYTLAGFGPTGIGFVCGIVAYTGANIAVSRRGGKHRKASKGSQAS
                     EGEVSGSGLALAIGALLDGIPESMVIGVSLVGGHSVSLVAVIAVFLSNMPEGLSSSVG
                     MKRAGRSAAYVFGVWGSIALISGLAALIGYVVFRNGSPVAVAATSAVAAGAILAMVVD
                     TMVPESFAEEHELTGLVAGLGFLTAFALSRWTR"
     gene            complement(9654..10673)
                     /locus_tag="VFQ61_14075"
     CDS             complement(9654..10673)
                     /locus_tag="VFQ61_14075"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9955636.1"
                     /translation="MSFRVATYNAGLAVGLLPYATERAERVVTALSALEVDLLFVQEF
                     WLDSHWEFLRRALAPRLPYDARPSPILKRTAACQAEQLRPLVECASRWCAGRRDEDLA
                     RCVVEHCAPTALTLPTPCLNCIASHPEGTLEEIVGRCVGNSADSVSAGLGSSRYGGLI
                     AYGGSFGTGLIARTPLLDVDQLVFESSVNARGAIYARLGVPAFGELAVIATHLSPGGA
                     EHPPQVHRLLSWLADKAPNGPAILLGDLNMTPANPLFRLLESAGFSAPPPPHGFATYT
                     PNGLGTGEAPTAGIQLDHILLREIDAPAAARRVLDQPIHLSVGEQTVLTTLSDHFGVL
                     VEFDS"
     gene            complement(10739..11827)
                     /locus_tag="VFQ61_14080"
     CDS             complement(10739..11827)
                     /locus_tag="VFQ61_14080"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9955637.1"
                     /translation="MKPANWPAVVILRPCEGSDSELEANLLSTATAHYEGPRRVLVLV
                     PSYQDPAHSAAEWARAELFRRGIDVPFEVRVSSIRTDKNRKVAQLASVEAELHEEVIV
                     SADSDIRFDDDTLPALVRALVADPSCAAATAPQVEVAPRTLGDWASAALLSSTPHSFL
                     CLAGLAERARGSHNLCGAVVAYRSGPLREVGGFHALESYLGEDFEVARRLHERRYGIA
                     TVAVTCGNYDSGRSLREVVQRFARWSIVTRRQRPELHLTYFVFLGCTPLLAILTAVVA
                     WTQAPYWTISVGYGLISIAARLILAAVLRGHAGLKRDPLTLLANVLAGELLILAGALG
                     ALRATEVQWRGHRYRVHAGGHIERLHVA"
     gene            complement(12344..12577)
                     /locus_tag="VFQ61_14085"
     CDS             complement(12344..12577)
                     /locus_tag="VFQ61_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955638.1"
                     /translation="MIELRGELAGGLAEENTTGHGLYDFRVAGQNGLMPFGPLRIEAL
                     KSPIGVKSGNTIGVFEEGFEDWDRIGLSGERER"
BASE COUNT         2384 a         4054 c         3885 g         2432 t
ORIGIN      
        1 gcgttcggcg ggatcggtca gaaacttcgc gacggtgtat tcggccagcc ggtcgtcgta
       61 gggctggaag tagccgtagg caccggcgcc gcgcgcgtgc accacgcgtt cggggatgcg
      121 ctcgtgatcg aagtgggtga tcttctcgcg caggatgaaa tcctcgagca gagtcgggcc
      181 gcgcgggaag gctttgagcg agttctgatt atcggcgatc gcgacgccct gattggtcgt
      241 cagcgtcgga ttacccgcct cttgatattg ctgcgtctca ccaccgttgc cgcgtgacgc
      301 actctccaga ggctgattgg cagtcgcact cgattcatcc ttctcggtcg tgatcgcagt
      361 cttcttgggg ctcatccgta gtgctccgtg gcgctgtgtt gcggcattca gcaacgctcg
      421 tgccaatcgc gcctcacgcc aacacgatac gaatgctcga atgtctaatc ggatcggggc
      481 gcactgactc gactggcctt cgcgaaaacg cctcacccac gcacaaacga cgcaacgcaa
      541 cctggctcta actgctttcg ccgttcgata acgtgcgcga cgctcgtcga gttcacgctc
      601 ctgcgcagtc actcccgccc cctcgtcccc caaacaccgc gccggcgccc ccaataactc
      661 agacttcgaa gcgcaacctc agtaatacgt cagccccgcg aacgcagcga tgctcgcggc
      721 gtgcccgctc atctctgcaa acggcagata gtcggcgcgc acaccgatcc ctgcgccgag
      781 cgtgcgcagt gtccaaaggt cgtaacccac gctcccaccg accaataagc ccctcatcgt
      841 gttgtccaca cgtgatcctc cgacgacctc atcatgccac gcaatccgag tagaagctcg
      901 ggcagttggg cttcctgttc tcggacatcg ggtattggag actcggcaac tgcggagcat
      961 tcgtgcgctc ggtgggcggc tctacggcgg tgggctcgcg aggcgtcgcg caggcgcttc
     1021 tggcacgcct ccacttgagc ccgggtgtcg ttcgcgaagc gaatcagacc ttcgaattct
     1081 acgggctgct gagttcctgg ggcggtcgag agctcgcgca gccgagtgtt gcccaaaccg
     1141 tcgccaaaga agcgaacgcg caccgcaact gagatccgcg cggtttgcgt tgcagggggc
     1201 cttgccggtc ggcgcgctaa atggtcatgc gcgccgcttc ttcccgctcc gggtgcggtt
     1261 gtcaatgtcg atatggatcc ggaacgcggc gggtgacgcg cgccgactca ggacgttgcg
     1321 ttgcagggcg gaacaggcgc ggcaccaggt cgcgactgag ggcggctgcg actgcgcaaa
     1381 cgagggcaat cgacgcgcgg cgcgcacgta acaagggccg gcccaaaccc ttctcgaagt
     1441 cacacgggag acggagcgag cccgcgttcg cccgggcgtg caggcaagtc gcgactccga
     1501 gaagcgtttc gaagcgcggg cacacgaggg ctccggtcac taaccgcggg gcagtgtgcg
     1561 ctcggcgcag tcgaaacgct tctggtggct gagctcgcgc gcgcccccca agcgcgtcga
     1621 actcggtcgt actgttggat gcggtggctt ttaggtccgt ctaggcttgg gtcgagcttg
     1681 gctacgaact ctcacccacc ggcaattcgc acgcggtggt cctcagggag tggtttcagg
     1741 agccggggtg gttgtcagtc gccgagaccg ttctcgtcga acccgccgaa gcgatgctcg
     1801 gagggcgccg cgtgacggcg atccctagcg cactcttcac aaacgtagcg tccgtccgag
     1861 gtcggacgca ggcggtggtg acgttggcag cagcagcaac gggtcttttc gatcgtcatg
     1921 ccggaggagc tatccttcga caggtcctcg cgagtgacga tgccgagcac gcgatcgccc
     1981 tgcgtgacga gcagtgatcc gacgcgtttt tcgttcatga tttgcgccgc ggcccgagtg
     2041 ctggtctctg gagcagtcgt cagaacttcg cggtgcatgc aggaacgcaa cgaatcgttg
     2101 agggacgcgc gctcgagatc gcaagtgcat acgaagcctg ccaaacgccc ggcgaaggtc
     2161 agcgggaagt gatggatctt acgctgcttg gcgagcatca acgcttcgtg aacactggca
     2221 tccacatcga gactgataac cggatgggtc atcacgtttc cgacgcagat ctcatcgtcc
     2281 gttgcgggtc tatcgagtga catgatgacc ggctgcattg cacggcgcgc gccaatctcg
     2341 aaagacccag aaaccccgca agacccgatc cggcgccctt aaagacttgc agcgcagagg
     2401 gccgcccggc gcagaccttg cgcgaaaagg tcggttttct tggcggattc cgtcgcagag
     2461 gctgccccac gctgacacct ctcatgtctt tcggctggca cctgcgcgtc ggttgcctgg
     2521 cgctccccga ctcgccgttg tgcgaatgct gtttgcttcc ggacacgggc gcgctacgca
     2581 ccccgggcca tgggcgccca atggaaacaa aaaggtcgtg aacagagcgc cgacgccaag
     2641 ggtcggttgt tcacgaaact gtcgaaagaa attatggttg cagcgcgagc tggagggccg
     2701 gatccggccg tcaatccgcg gctccggtta gccatcgagc aggccaagcg tgcgtccatg
     2761 actcgcgata cgttggagcg cgcaatcaag aagggcgccg gcttgctcga cgccacgaca
     2821 caatacgaaa cggtcgtgta cgaaggtttc gcgccacacc aggtgccggt gatcgtcgag
     2881 tgcctcaccg acaacaagaa ccgcaccgcg ccaaatatgc gtcaactctt ccgcaaaggg
     2941 cagctcggct cgagtggctc ggtgagctgg gacttcaacc gtcggggtgc gatcgaagct
     3001 tcgccccctg cgggcggtca ggatcccgaa gaagcggcga tcaatgctgg cgcgcaagag
     3061 gtagaagcag gcgaggaggg gcagagccgc ttcatcaccg acgccacgga cttggatgct
     3121 gtctccaaag cgctcaccgc gagcggttgg aacgtcacca gcgccaatct cgtttggatt
     3181 gccaagaacc cggtcaagct ggagggtgct gcgcgcagcg aagtcgaggc atttctcgcc
     3241 gcgatcgacg acgacgacga cgttcaacac ctctatgtag ggctcgcctg agtcaagcgc
     3301 cagtccggta gtgcaactcg gagggatgag gatccatgct caagctcgtc ggcgcgaacc
     3361 tgaattattc gtcttggacc attcggccgt ggttggccct cacccactgg ggaatcgagt
     3421 tcggtttcca cgacgtgggg ctcaagactc gtccggattg gagggagcgc atcctttctt
     3481 tctcgggcgc gggcaaagtg cccatcctga tcgacggcgc cttgagcatc cacgaatcac
     3541 tcgccatttg cgaatacctc tccgagctgc atccggccgc caagctctgg cccggtgatc
     3601 cgcagttgcg cgcccgcggc cgcgcgattt catgtgagat gctctcgagc ttccaggctt
     3661 tacgtagcgc catgaactgc aacctgcgtg gccgcgccaa gcagacgccg cgttcggccg
     3721 cgatcgacgc cgacgtacgc cgcgtgctcg aaatctggga cgcctcactg cagtcctcgg
     3781 gtggtccgtt cctgttcggt gagttcagca tcgcggattg catgtacttc ccggttgcga
     3841 cccgtttccg cacctacggg gtggaactac ccgagttcgc gcgcggctat tcacgagccc
     3901 tcttcgagtt gcccgcggtc cagaagctcg aacaaatcgc gagtgcggcg gttcccatcc
     3961 ccgagtacga cgtagctttg ggctgaacat caccgcgaat cgatccgcac caatgacggc
     4021 gaaatacgta ttcatggccg gaatcgggaa ttccgagccg gaccattggc aatcactctg
     4081 ggcccgttcc actccgggct cggtttgggt cactccgcag agttggagcg aaccacagcg
     4141 tgacgactgg gtcgccgcct tggtcggagc ggttcgccgc gtcggtgagt ctgccgtcat
     4201 cgtcgcccac agcttgggtt gtctggccac ggccgaggcc agcgcggacc ttcgccaact
     4261 cggcgttcgc ggcgtgttcc tcgtggcgat cccagatccg gcgagcccgc gctttccgag
     4321 ctgcgctcgc ggcttcaagc ccgctgtcgc ctgccacatg gccgtcccag ccctggtcgt
     4381 cgccagcacc accgaccctt acgccggcct cgagtatgcc gagcaaaccg ctcagcactg
     4441 gaacagccgc ttcgtatccg taggcgacaa gggccacatc aacctcgcct ccaatctcgg
     4501 agagtgggaa gagggccgtc gattgctgaa cgacttcgtc gcgacgctct gaggcgctga
     4561 ttaagtcggg ggttcaccgc cggcaaggaa gctcagccac ttcggcagta cttcggagta
     4621 atcgggcgga aacgcatgca tcgagacgcc cgggtacacg tgatagtaaa cctgggcccc
     4681 gaactgcaac aatcgttcat tcgagttctg ggccgtagcg aggacacgcg cgtgctcctt
     4741 ctgccccaca acgatgacga agcgtctgcc cgataacttg gagtcggcga acgcaatccg
     4801 ctccatcgaa gacactgttc ccggtgacat cgcaatcgcc cctctaaacc tctcgggttg
     4861 ccgggccgcg atctccaagg ccagctgacc accttgtgag aatccaatca gatagatttg
     4921 cccgctcgtg accgttacct gctcggacac cgccgctatc gcacgctcga ttcgtgatgt
     4981 atcggccaac acgtcctctc gccaggcgaa gctctccggt ccgcgggtta tggttgcgct
     5041 gacggccagc agcgccattc cgagcgcatc cgccatcaac tcgaagtcgc cactgaagtc
     5101 ctcgggcacg gagccgaagc cgtgcaaccc gatcaacatc ggaatcgggc gcccaactcg
     5161 atagctcttg ggtaagctca aaatttggcg gtcgagcggg tgatgctcat agtactccgc
     5221 cattcggtcc aagtacgaga acaaggttgg cccgcgcttg tcagcccgca cgagcgcgag
     5281 gtctggatcg cgggcagccc atcccggatc tacgccgtcc tccttcgccg cgcgttgcag
     5341 ccaatagatt gcggcgccga cgtcccctga tctcgaatag ctgcacgcga gtttgtactt
     5401 cgttcccccg tgggcgttcc gccgaacgct gttgtactga cgcaggagtt gttgctgttc
     5461 aggagtcaat tccaagaccg gtgggggttg gtctagttgg aaaggagcaa cgggcacgcc
     5521 gttcgcatcg gtggagatgg caatgacgcc agcaactcga ggcgcggacg ggtcgatttc
     5581 ctggaccgtc ccctgggaag agcacttgaa tacgaccccg aaaatcacga gaccaatgaa
     5641 gatcccccac tgaagcttca agcgatggtt catcgacggt aaaaccatga gacgccttct
     5701 agtgcagcgc atataaaaca gcgacaagaa gcttcgcgcg ctgcaccgct caggaaaaag
     5761 gctagacggc gcatgccgcg aaagcggacc atcgcgaggc gatcgttgca gcgggtcacg
     5821 tccgcggcgc tggcaggcct ggtcgagaac gaccgagccc acgacaccgg gccagaaatc
     5881 tcggccaatg tttgcgcact ttctcgccaa atacggcggc ggtcgcagcc ggcgctcgaa
     5941 atgtctgggg ttcgcgctat gggtgaacga tggcccactc aaaaatccga atctgcatgg
     6001 gaactctgga atgttagaac cttgccgcgt ttgcggaggg gacgggcggg ttggaaacgc
     6061 attcggaggc agtgcgacca cttgccctgg gtgccgtggc acgggacgtc gctccaacac
     6121 cgaatcgcta ttccacgatg tcacgaagac caagccctct caccatgggc ccgcaaccaa
     6181 gccgggcgcc ccggccaaga tcaccgctcc caccacgatc gagggcgtct cactcggcaa
     6241 tgaagtcaaa gcctctggcc tctccgaaga caccaaagcc aaactgattc gggaaatcat
     6301 cgagtacgag gttagccacg gcaaatgcac gcagaccttc gtcaagaagg tccgcaaaca
     6361 gatccgtccg gcgacttgag ccactgcatg cgcgagcact aatcgctcag ggcgtgagtt
     6421 cgaactagga ggcctcggcc agttcccgct cctcgtcggc tcccagctgc gcgtcgagaa
     6481 ccagttgctt gccgatccaa gcctgcagtt gggccggatt ttcgcggatc agcttgtcga
     6541 tagcgataga ctgcttgtac tcggtagcgg ccgcggtcat ggtccagctc gcttgaatgc
     6601 cgagcttctc gacctcactg cggatggtcg cgaggggcag ccgaaagaac tccttgcgtg
     6661 gattgacctt gttcatctgc gacgtcagga agtgtcgatg tagagcgtgc tccagcgcgg
     6721 gcgcgtcctc gctcgagatc attgcgtgca catcgaactc gaacgggacg ctcgcgtcac
     6781 cgagttcacg gatgcgatcc aggggctcga ggcgacgagt gagtcctatc ttgaacacct
     6841 cctccccaaa tgatccgacg ttcgagatga tgtacacgtg gccggtcttg gtctgttggg
     6901 ccatcgataa cgcgcgctga ttcttctcct cggcggcggt gagcttctgc tggagctccg
     6961 cgagctgggc ctcgtagcgt tggcgctgtt cgttgctggc tttggccacg ctctgctgaa
     7021 tcttctccat ggccttcttg atcgcctctt cttccttctc tgcctctcgc atcgcccgct
     7081 cgtaatcctt cctagcccgc tcttcttcgc ggatgcgctc cttgattcgc cgctgctctt
     7141 cgcgctctcg gttcttcaat tcttgggctg cagccgccca ccgaagctca tcgagtcgac
     7201 tggcgagata cagatcggtg atgcgggcgt tgcggaacgc ttgcccgttc atgttcacga
     7261 gcgcgaatgc atctaggatc ttctgccgca aggtaccaac attgtcctcc ttgacccggg
     7321 acaggacgga atcgaccttt ccattgaagg catccgagat gaagttgacc gccgtctcgc
     7381 gacgattggc ctccacgtag tcacaagtcg ccgctcgccc ttccttgagc agctggcgtg
     7441 tcctctccct ggccaatttg aggttggcgc ctgcctcggc aaatccaaac tcggccgcta
     7501 agtcgtccag cacactgtgc gccggtacga tgtactggtc tccgtaacct tcgatcacgt
     7561 tctgcagagc ccgcgcctct cggcgattct tctccaggct ggcctgagca cgcagcgctt
     7621 cgccggctac ctcttgagcc cgtcggtccg cttcggacag gattgtggcg tgccgctgtt
     7681 ccgcggcctg aagggtagcc tcggcgcgag ctttggcgtc cccgagcagc tgttgtgcct
     7741 ccttgtgcga agcgcccaag gtgccaatgg cttgagcttg ggcttgggcc agaatcgatt
     7801 gagcctgttg ctcggacgac tgcaccgcct cggtcgcctg gcgattcgcg tggaacagca
     7861 gggattgcac ctgctcccgt gctctttggt gctccgcctg cgcagtcgct cggatctcgg
     7921 ccgcctcccg ctccgcgtca accacttgac gatatctcga caggagactc acctcctgct
     7981 ccgcaaccgc cgcacgtgac tccgcggctt gtctggcgtt ggcctcacca tcgcgctgcg
     8041 cgactgcctt ggctcgttca ctcgccagga tgatcacgat cacggtcgcg atgcccacga
     8101 tcgctaaaat caggccactg atcagcagga ttgaagtcat ttggggatct tcttctctcc
     8161 gtcggccgag ccgcgaacca cttcgagttc tcgattcgcc aatctagcgc tgcgtctccc
     8221 gcccgaagcc gtcccgggac ccatttaccg tcgaacatcc caaggcgctc cgctagcgcg
     8281 cagccgacac gtgtgaattc gacaactcta agccctttgc ccgcctcctt caagacgagc
     8341 cgcgtgagac ttcgccccgg cgtcaactcc ctgttggcgg tgtgccgtcg ctccgtgggc
     8401 gctctctccg cgttctgtca gcactctgtc agtactcggc gattaatact attgacgctc
     8461 agcctacaac tctgcttcgt tagtcattta cacatttgga cgcacacaga tgaccgcggc
     8521 ggccgcaatc accgggagcc gctagcggag tggactgaag aacccgatcg tctcgcgggg
     8581 ccctcttgtc ggaggcagaa gcgcgaggac ctgtccgagc gaaccccggc gatccagatc
     8641 cggtgtcgtg actatacttc gaccgaaggc gcgggcgtga aatgacgctt ggagcgtggt
     8701 cacgcttgtg agtgcgctga tccactcgag tcggcgcttc gcacaggtgc gcttgcccct
     8761 gcgcgcggcg tgatggtgac tttgcagctc gccagcaagg ggctagtctc gacgtcgtcc
     8821 ctttatcggt atcgacgact cgatgcgaaa acaggaacag gaacaggaac aggagcagag
     8881 ccttgcccgt cgcactatta gctggattgt ggggattggt ggcgggcgcg gcgctcgtcc
     8941 tgggagcgct gatagggtgg ttcgtggatg ttccgcgtcg agtcgtggcc gcgattatgg
     9001 ctttcggcag cggggtgctg atttccgcgc tctctttcga tctgatggac gaagcgtata
     9061 ctttggctgg ctttggaccc acggggatcg gattcgtttg cggaatagtg gcctacacgg
     9121 gcgcgaatat cgcggtctcg aggcgcggtg gcaaacaccg caaagcatcg aaaggaagcc
     9181 aggcctcgga gggagaggtg tccggtagcg gtcttgcgct tgcgattggc gctctcctcg
     9241 acggaatccc ggaatccatg gtgatcggcg tgagcttggt cggcggtcac tcggtcagcc
     9301 tcgtggcggt gatagccgtc ttcctctcga acatgcccga agggctctcg agctcggtgg
     9361 ggatgaagcg ggcgggcaga tcggcagcct acgtgtttgg ggtgtggggc tcgatcgcat
     9421 tgatttccgg gctcgcggcg ctgattggct acgtcgtatt ccggaatggt tccccggtag
     9481 ccgtcgccgc cacgagcgcg gtggcagcgg gagctattct cgccatggtg gtggatacga
     9541 tggtacccga gtcctttgcg gaagagcatg aactcacggg tctggttgcg gggctcggct
     9601 ttctcactgc atttgcgcta tcccgctgga cccgttgacg tgcggagttc acgctaggaa
     9661 tcgaactcga cgagtactcc gaagtgatcc gagagagtcg tgagcacggt ttgctcgccc
     9721 acggagaggt gaatgggttg gtcgaggacg cggcgagcgg cagcgggagc gtcgatttcg
     9781 cgcagcagaa tgtgatcgag ctgaatacct gctgtcggtg cctcgccggt gccgagaccg
     9841 ttcggagtat aggtagcgaa gccgtgcgga ggaggaggcg cgctgaaacc ggcgctctcc
     9901 aaaagtcgaa acagcgggtt cgccggcgtc atgttcaagt ccccgagcag gatcgcaggc
     9961 ccattggggg ccttgtccgc gagccagctc aataagcgat gtacttgggg tgggtgttcg
    10021 gctcctcctg gcgataggtg agtcgcgatc accgcgagtt cgccgaacgc agggacgccg
    10081 agcctcgcgt aaatggctcc acgcgcgttc acgctggatt cgaataccaa ctgatctacg
    10141 tccagcaatg gagtacgagc gatcaaccca gtgccgaagg agcccccgta agcaatcagc
    10201 cctccgtagc gcgatgaccc cagcccggcg ctcaccgaat cggcgctatt gccaacgcaa
    10261 cgcccaacta tctcttcgag tgtgccttcg gggtggctcg cgatgcagtt caaacatgga
    10321 gtgggtagcg tgagcgcagt gggcgcacag tgttcaacga cgcagcgcgc gaggtcttca
    10381 tctcggcgcc ccgcgcacca gcggctggca cactcgacca atggccgcag ctgctcggct
    10441 tggcaggccg ccgttcgctt caggatcggg ctgggacgtg cgtcgtaggg caggcgggga
    10501 gccagcgcgc ggcgcagaaa ctcccaatgc gagtcgagcc agaactcctg tacgaacagt
    10561 agatccacct cgagcgccga cagcgccgtc acgacgcgct ccgctctctc ggttgcataa
    10621 gggagcaagc ccaccgcaag ccccgcgttg taggttgcga cgcgaaatga catgggtcca
    10681 cctttcgaat cgagtctcgc actcagctcc gatgcgacgc gcggcgatcg tgcccgaatc
    10741 acgccacatg caacctctcg atatgacctc cagcatgaac tcgatagcga tggccccgcc
    10801 actgcacctc ggtggcgcgc aaggcgccca gagcgccggc cagaatcagc aactctccgg
    10861 ccaagacgtt cgcaagcaag gtgagagggt cgcgcttcag cccagcatgg ccccgcagaa
    10921 ccgccgctaa aatgaggcgc gcggcgatcg agatgagccc gtaaccgacg gagatggtcc
    10981 agtacggcgc ttgggtccaa gccaccacgg ccgtcagaat cgccaagagc ggcgtgcagc
    11041 cgagaaatac gaagtaagtg aggtggagtt cgggcctttg tcgacgcgtc acgatgctcc
    11101 aacgagcaaa gcgctgcacg acctctctca agcttcgacc actatcgtag ttgccacatg
    11161 taacggcgac cgtcgcgatc ccgtagcgcc gctcgtgcag gcgtcgcgcg acttcgaagt
    11221 cttcccccag gtaactctcg agtgcgtgaa aaccacccac ctcgcgcagc gggccgcttc
    11281 gatatgccac gaccgccccg cataggttat gactgccgcg cgcgcgctcc gccaaccccg
    11341 ccaaacacag gaacgagtga ggcgtggacg agagcagcgc ggcagacgcc caatccccta
    11401 acgtgcgtgg cgcaacttcc acctgcggcg ccgttgctgc ggcacagctc ggatccgcca
    11461 ccaaagcccg aaccaacgcc ggtagcgtgt cgtcgtcgaa gcggatgtca ctgtcggcac
    11521 tgacgatcac ctcttcgtgc aactcggctt cgacgctcgc aagttgcgcc acctttcggt
    11581 tcttgtcggt ccgaatgctc gaaacccgga cctcgaacgg cacgtctatg ccacgtcgga
    11641 acaactccgc ccgggcccat tccgcggcag aatgggctgg atcttgatag gacgggacca
    11701 acacgagcac ccggcgtggt ccttcgtaat gagccgtcgc cgtggacagc aggttggcct
    11761 cgagctccga gtcagagccc tcgcacgggc gcaaaatgac gactgcgggc cagtttgcgg
    11821 gcttcatcgt tgccgcgcgc cgcgtgcgcc atatcagcct cagcgcccat ctcgaggcaa
    11881 tgaccgaaaa ccctaaagca aagcccgccc aaacaaagag caccacagag agcgcgagta
    11941 gtgctgccgc catctgacgc ccatctggag caatatgcgt gcctccccga aacgcccatt
    12001 acgacacaga gataggcatt cgcccgttgc cccggattag actcttccca tgtctaagcc
    12061 gacccgagga acccgcaccg acagacttca ttggttgatg gtgggagtcg cgatcctagg
    12121 gtgcagtcga ggcgccccgg ggtcttctgg cgttagcaaa ccagtcggat ctgaagcagg
    12181 tcagttgcgc gggtctgcgt cgagccagtc cagcgccacg ccccgtgcag accgcgacag
    12241 ctccggactc gaactatccc aagccgcccg gaaactcgcc gccgtcgaaa ccttcgcgtt
    12301 gggtggagtc ggtatcgccg gagagcagag ccagggtgag aggctaacgc tcgcgctcgc
    12361 ccgagagccc aatgcggtcc caatcttcga aaccctcctc gaacacccca atcgtgttgc
    12421 ccgactttac gcctattggg ctcttcagag cctcgatccg gagcgggcca aacggcatca
    12481 agccgttctg gccagcgacc cggaaatcgt acaaaccatg tccggttgta ttctcttcag
    12541 caagcccacc cgcgagctcg ccgcggagct cgatcatttt ccccgaccca tacctcagcc
    12601 ttagtccgag cgtcaggctt ttaacaaggc cacttcgacg ccgtactcct cgaacagcgg
    12661 cccgaggcca ccgtcgctgg cgagagcctt gttgtcgacc gacttggtcc aggatcggcc
    12721 gagatgctga gagccgaatg agagatgttc gacgg
//
LOCUS       DASTKU010000410         8887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_968427, whole genome shotgun sequence.
ACCESSION   DASTKU010000410 DASTKU010000000
VERSION     DASTKU010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968427"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFQ61_14090"
     CDS             <1..456
                     /locus_tag="VFQ61_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET9955639.1"
                     /translation="YFARARVHLPVFPGAAALVARRARLGPVGIVSGALRDEIELGLQ
                     TLAVAEHVGFIVSAEDTSACKPDPEGYLRGLSELTNRIGEDAARRALVIEDSLAGVEA
                     ARAAGLTCVAVAHSYPKARLEAAGADCVVERLDELSDSLLESLYQRVHG"
     gene            548..1759
                     /locus_tag="VFQ61_14095"
     CDS             548..1759
                     /locus_tag="VFQ61_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955640.1"
                     /translation="MPALLAATTVALRVAQSEPEISAPSPPPTAQTASAQAIGIQASV
                     STLDFGLPPDFRAMRGVTLGPIESSLHPNRGYGSPAFTQAVSEVRRMGGSWISITPFG
                     RVLNLKPTGVALTFEAPFAQNRDALLKATRQAHAAGLKVLMVPHLWVETGEWRGEIEP
                     GDDAAWERWSRGYLEFVSAWAELAREAQVDLFAVGVELRSWLTTARAPSFQRVIEEVR
                     RIYQGPLTYAANWDDVEETTILGSLDYIGINAFFPLAEQPNAPKAALLERGRAVAERV
                     GQLAKAWQKPVIFTEFGYTTRPDPTLRPWEWPDSMKNVRVDQAAQAEAYEALLAPMID
                     QPWFAGAFVWRMYADPNDLSQEAEWGFSPRGKLAELTLRDAFAAHWASDGPRAVGASL
                     ASSGAERIGLY"
     gene            complement(1773..2915)
                     /locus_tag="VFQ61_14100"
     CDS             complement(1773..2915)
                     /locus_tag="VFQ61_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955641.1"
                     /translation="MSCLDMPGITDLGRYGATSAGEEESVANCFEAPSDYAELVQSPE
                     SGIELSIEDAFVVDQRAGLNANLTSLVPWLPHVGIHNDRNQKLTARVTLKEARFVTLV
                     GLASKLQGQKRERQCLEALCRADYTYVQKALVGIPSVVIRAENSTGKSAELGAWAVSG
                     DFAQRELSSGARELTSNKPVTLAIARGAFRTPQTERLCQFCGKRGQGCCAEAPSCDGG
                     LACIANQCVEVGGPGQPCDGESCSAGATCVRGRCELECGGRGQPCCGTSECSGRLRCA
                     EDPEDGLEHSVASQDVSVGGGFFGTSEDRTFGSASCGPLMRRQRFAVTKVAEGRGDCQ
                     KAWWFDPKNERDCRVAVHFEVSTFGTASCHVDVFALKPPKPRRCSP"
     gene            complement(3170..4867)
                     /locus_tag="VFQ61_14105"
     CDS             complement(3170..4867)
                     /locus_tag="VFQ61_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955642.1"
                     /translation="MRRVNTVSLISGVLAFVGAGSMLASSCAPTNLGCDAYLTCDTND
                     SRGGAAGDTGLPGSAGAGGSNGGAEQESGGAAGAGAAGASTVGGNTDCDTQASPSAAP
                     CLVDERYGVFVAPEGDDELGDGSRSAPFGSLQHALEVAVRVQRSRVFACATAGAFEEA
                     LAVDEALDGAALYGGFSCDDWSRVEQRTRVESPESWGARSEGLRRGFTAEDIEWVAAD
                     AAQSTAEGTAESSVALWVLESKGIVLRRVRLQAGAGGDGAPGEAGQSGRDGAPAPDAL
                     SGQDADCSEAAPTSQIGGEWPAEAVACGSSGGAGGQAFKSAVADGNRGSDGVPRTHVT
                     PANEHNAGDGATEVAQNAGSGRQGSNGDRGEPGAAAEELGIFTLEGYEPASGVPGTPG
                     YAGQGGGGGGASKGDGSCLGASGGGGGMGGCGGNPGTGGGGGGASIAVLSWNSSIRIE
                     SCELRAGDGGTGGEGGSAGPGGLGSAGAAGGLGDTVAGIGDGGQGGKGGHGGAGGPGS
                     GGSGGPSLALVFKGERPDLRDTDLVLGRAGGAGLGGKAGAERAPSGADAEAREVLEL"
     gene            complement(4871..6001)
                     /locus_tag="VFQ61_14110"
     CDS             complement(4871..6001)
                     /locus_tag="VFQ61_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955643.1"
                     /translation="MRTCSKEGRKPSLTGARRWIATLCSSVFLGPVAVPATSHALEPA
                     ILGAGATSSNGSSLDLNPIYRKAKVAMDQEDWQTAFNLMLQVWEHRRSADVSVNLGLI
                     EIELGRWRDAAEHFAFGLSHMPPDADPLLVELNADRLAECQKHVATLILDVEPASAVL
                     SVAGRVLEPSPVTPRQIFAEPGVLAVRAELEGYEPQSALVTAKAGEQLRLSWALRERL
                     QPIIVADPPRNLPPHPGFEWWPLAISGGLSLLAVGAGVGFHFDAAAARADADQLRGAL
                     RSELGADACWNVELAACRELADLRDRQASSNRASNAAFISAGVIGLAGFAVQMAVHRP
                     KWLGTSSDHQAAKPHPFLPRRAGFELPSISVSKNGVELSGRF"
     gene            complement(6148..>8887)
                     /locus_tag="VFQ61_14115"
     CDS             complement(6148..>8887)
                     /locus_tag="VFQ61_14115"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9955644.1"
                     /translation="TWRFRSSPRFESGMARSESELILIGGVSISLLSFALTWVLARTR
                     ARSVRLATEMTRTIRENQDRLSRLNALQSAILDGAPYAILSTDTQYTVRTMNPAAQRM
                     LGYSAQELARGIPLAELGVSSRERPPLPARNSTGRWATWDSILSAAERGSRADGELRL
                     VKKNGDEFVALVSMVNLARAAEGSGGSVIIITDISERRAQESALAERAALLEMSSRIG
                     LALSASDSMRTVLEGCARILLEGLGAALARIWTLDASGKVLEMQASAGLYTHTDGSHG
                     RIQVGALKVGAIAESRTPFLLNSVYGDSRIPDQDWARREGLVAFAGFPMLLGERLIGV
                     VAVFSRQTLSQTAWASLGAAADALAIGIERLRAAAGLEQKARELADAAAAMALARDAA
                     DDANRAKSEFLATMSHEVRTPLNGVIGLTGLLLDTPLSPEQREYATLVRKSGSALLAI
                     INDILDFSKIEAGRMLLEAIAFDLADWVFDTLDILAEQAWEKGIELNGVIDPELPRTV
                     IGDPLRFRQVLVNLVANAIKFTPYGEVLVAISALALSNELLTIRVSVRDTGIGIPLEI
                     QPRLFRAFSQADGSTTRKYGGTGLGLAISKRLCELMGGEIGFSSVPGNGSEFWFEIKF
                     GLPAEQRGEASDSNRLVGRLCGVSGSENTLAAMASSAQRLGLPFSGFRSAAEAIHGFR
                     QIDTGLPILAVLVDEVLPDSDGVSSCGALRGIDKLKAARFMLLVSGTPRGEVLRAQER
                     GVFVCVAKPVRDHRIVSGLQESRAEALVIAEEPRPVQTSLSRALAPSRAAATGDRARV
                     LVAEDNMVNQRLLARLLEKRGIFVDIVPNGREAIQAATRASYGLILMDWQMPEVDGLE
                     ATAAIRQAGCRVPIIALTASAMKGDQERCLAAGMDDYLAKPIAEADLFGILDRWLP"
BASE COUNT         1559 a         3167 c         2669 g         1492 t
ORIGIN      
        1 tatttcgcgc gcgcgcgcgt tcacttgccc gtattcccgg gtgccgccgc gctcgtcgct
       61 cgacgcgccc gactcgggcc cgtgggcatc gtctcgggcg cgctgcgcga tgagatcgag
      121 ctcggcttgc agacgttggc cgtcgccgaa cacgtggggt tcatcgtgag cgcggaagac
      181 acgtccgcct gcaagcccga tccagaaggg tacctacgcg ggctgtcgga gctcacgaat
      241 cgcatcggcg aagatgcggc tcggcgagcc ctggtgatcg aggattcact ggcaggagta
      301 gaagcggcgc gcgcggccgg gctcacctgt gtcgcagtcg cgcatagcta ccccaaggct
      361 cgactcgagg ccgctggcgc cgactgcgtc gtggagcgcc tggacgagct cagtgacagc
      421 ttgctcgaaa gcctgtacca gcgcgtgcat ggctgactcg acaggtcgcc cgcggcgggc
      481 ttggttcgcg atgcacactc gggctccgcg gatcgaatcg cccagggtaa aaaagcttcg
      541 acacgtcatg ccggccctgc tcgccgcgac caccgtcgcg ctgcgcgtcg cgcaaagcga
      601 acccgaaatt tcggcccctt ccccaccacc cacggcgcag accgcgtcgg cacaggcgat
      661 cggaatacag gcatcggtat cgacgctcga cttcgggtta ccccctgact tccgagccat
      721 gcgcggcgtg acgctggggc ccatcgagag cagcctgcat ccgaaccgcg gttacggctc
      781 ccccgcattc acgcaagcag tctccgaggt gcggcgcatg gggggcagct ggatcagcat
      841 caccccgttc ggtcgcgtcc tgaatttgaa gccaacggga gtggcgctca cgttcgaagc
      901 accgttcgcg cagaaccgcg acgcgctgct gaaggctact cgacaagcgc acgccgcggg
      961 cttgaaggtg ctgatggttc cgcacctgtg ggtcgagacg ggtgagtggc gcggcgaaat
     1021 cgagcccggt gacgacgccg cctgggagcg ctggagccgc ggctatctcg agttcgtcag
     1081 cgcctgggcg gagcttgcgc gcgaagcgca agtggacctg ttcgcagttg gagtggagct
     1141 gcgcagctgg ctcacgaccg cgcgtgcgcc gagttttcag cgcgtgatcg aggaggttcg
     1201 tcgcatttac cagggcccgc tcacgtacgc agccaactgg gacgacgtcg aagaaacgac
     1261 catcctaggt tcgctcgact acatcggcat caatgccttt tttccgctcg ccgagcagcc
     1321 gaacgccccc aaggccgcgc tgctcgagcg cggccgagcg gtggccgagc gcgttgggca
     1381 actcgcgaag gcctggcaga agccagtaat cttcaccgag ttcggctaca ccacacgccc
     1441 tgatccgacg ctgcgcccgt gggagtggcc ggactcgatg aagaacgttc gggtggatca
     1501 agccgcgcaa gccgaggcct acgaagcgct actcgccccc atgatcgacc aaccgtggtt
     1561 tgcgggcgcc ttcgtgtggc gcatgtacgc tgacccgaat gatctctcgc aagaggccga
     1621 gtgggggttc tctccgcgcg gaaagttggc cgagctaact ttgcgcgacg ccttcgccgc
     1681 gcattgggcg tccgatggcc cacgcgcggt gggcgcgtcg ctggcgagct cgggcgccga
     1741 gcggatcggg ctgtattgat cgtaggtcga ggttacggcg agcaacgccg cggcttcgga
     1801 ggcttcagcg caaagacatc gacatggcag ctcgcggtgc cgaacgtcga aacttcgaag
     1861 tgcaccgcga cgcgacaatc gcgctcgttc ttcggatcga accaccacgc cttctgacag
     1921 tcgcccctgc cttcggccac cttcgtgacc gcaaagcgct gacggcgcat caggggtccg
     1981 cagctcgcgc tgccgaacgt gcggtcttcg ctcgttccga agaatccgcc gcccacgctc
     2041 acgtcctgtg aagccacgct gtgctccagg ccatcctcgg gatcttcggc gcagcgcaaa
     2101 cgacccgagc actcgctggt cccgcagcag ggctggcctc gaccgccaca ctcgagctcg
     2161 cagcgtccgc gcacgcaggt agcgcccgcg ctgcaactct caccatcaca aggttgccca
     2221 ggcccgccca cctctacgca ttgatttgcg atacaagcga ggccgccgtc gcacgacgga
     2281 gcttccgcac agcagccttg gccgcgcttg ccacaaaact ggcagagccg ctcggtttgc
     2341 ggagtacgaa acgcgccccg cgcgattgcc agagtcaccg gcttgttgct ggtgagctca
     2401 cgagctccgc tcgagagctc gcgttgcgcg aagtcgccac tgaccgccca cgcgccgagc
     2461 tccgcactct taccggtgct gttctccgcg cgaatgacca cactggggat gcccaccagc
     2521 gccttctgaa cgtacgtata atcggcacgg cagagcgcct ccaagcactg gcgctcacgt
     2581 ttttgacctt ggagcttgct cgcgagcccc accagcgtga cgaagcgagc ctccttcagc
     2641 gtcactcgcg ccgtgagctt ctggttgcga tcgttgtgaa tgcccacgtg gggcaaccag
     2701 ggaactaggc tggtgaggtt agcgttcagc ccggctcgtt gatcgacgac gaacgcgtcc
     2761 tcgatgctca gctcgatgcc cgactccgga gattggacga gttcggcgta atcgctcggc
     2821 gcctcgaagc agttggcgac tgactcctct tcgcccgcgc tggttgcgcc gtaccggccg
     2881 agatcggtga tgccgggcat gtccaagcac gacatttccg ttccgattcg cgacgagcga
     2941 acggtcttgt cgagcatggg ctgaagtcgc tcgcaatacc ctgcctgcgg aggcaacgcg
     3001 accggtgcgg gcgcaggctg cggccgcggc gggctaccgc agcccatttc gagccctaag
     3061 gaggcaccaa tcaaaacctt tgaaagccag cttcgctgca aagcaacacc aggctgccac
     3121 ttgtaccacg cgcagggaag agttcggcga gtcgagttgg cgctggctcc tagagctcca
     3181 acacctcgcg tgcctcggca tcggctccgc tcggcgctcg ctcggcgccg gccttgccac
     3241 ccaacccggc tccgcccgcg cgccctagca cgagatccgt gtcacgcaga tcggggcgtt
     3301 cgcccttgaa tacgagcgcc aatgacggcc cgccgctgcc gccggagccc gggccacccg
     3361 cgcctccatg gccacccttg ccgccctgcc caccatcacc aatgccggca acggtgtcgc
     3421 ctagcccgcc cgccgcgccc gcgcttccga gaccgccggg gccggcactt ccaccctctc
     3481 ctccggtgcc accgtcgccc gcacgaagct cgcacgactc gatgcgaata gagctattcc
     3541 agctgagcac cgcaatcgac gcgccgcccc cgcctcctcc agttccgggg tttccgccac
     3601 agccgcccat tccgccgcca cctccgctcg cgccgagaca gctgccatcg cctttgctgg
     3661 cacccccgcc cccgccacct tgccctgcat agccgggcgt gcccggaacg cccgaggccg
     3721 gctcgtaacc ctcaagtgtg aatataccga gctcctcggc ggccgcacct ggttcccctc
     3781 gatctccgtt cgagccttgt cgcccgctgc ccgcgttttg agcgacttcg gtcgcgccgt
     3841 cgcccgcgtt gtgttcattg gcgggagtca cgtgcgttct cggcaccccg tcgctgcctc
     3901 gattgccgtc cgcaaccgcg ctcttgaatg cctgaccacc cgccccaccg ctggagccgc
     3961 aagccaccgc ttccgccggc cattctcctc cgatctgcga agtaggcgcg gcttcggagc
     4021 agtcggcatc ttggccgctc aaggcgtccg gagcaggggc accgtcgcgg cccgactggc
     4081 ccgcttcgcc gggagccccg tcgccaccgg ctccggcctg gagccgcacg cgccgcaaga
     4141 cgatgccctt cgactcgagc acccacaggg cgacgctgct ttctgccgtt ccctcggcgg
     4201 tgctctgcgc cgcatccgcg gcgacccact cgatatcttc ggcggtaaag ccgcgccgca
     4261 gcccctcact acgagccccc cagctctcgg ggctttcgac acgcgtgcgc tgttcgactc
     4321 ggctccagtc gtcgcacgag aatccaccgt aaagcgcggc gccgtcgagc gcttcgtcca
     4381 ccgcgagcgc ttcttcgaac gcacccgcgg tcgcgcaggc aaagacgcgc gaccgctgaa
     4441 cccggaccgc cacttcgagc gcatgctgca aactgccgaa gggtgccgag cgcgagccat
     4501 caccgagttc gtcatcgccc tccggcgcca cgaacacgcc gtaccgttca tccaccaagc
     4561 aaggcgccgc gctcggcgat gcctgcgtat cgcagtcggt gttcccacca acggtgctcg
     4621 cacccgccgc tcccgcgcca gcggctccgc cagactcttg ctcggcgcca ccgttcgacc
     4681 cgccagcgcc tgcgctcccg ggcagtccag tgtcgccggc cgcaccacct cgtgagtcgt
     4741 tggtgtcaca cgtcaagtaa gcgtcacaac cgaggttggt tggggcgcaa ctcgacgcca
     4801 gcatcgaccc cgcaccgacg aacgcgagca ctcccgaaat caaagagacc gtattcactc
     4861 gccgcatggc tcagaatctt ccggaaagct cgaccccatt cttggacacg ctgatactcg
     4921 gcagctcgaa gccggctcgc cgaggcaaaa acgggtgagg cttcgccgcc tggtggtctg
     4981 acgatgtccc cagccacttg ggcctgtgaa ctgccatttg taccgcaaac ccggcgagcc
     5041 caatcacccc cgccgagatg aaggcggcgt tggatgctcg attcgaactc gcttgccgat
     5101 cccgcaagtc cgcaagctcg cgacacgccg ccagctcgac gttccaacat gcgtccgcgc
     5161 cgagttccga gcgcaaggcg ccccgaagtt gatccgcatc cgctcgcgcc gccgcggcgt
     5221 cgaagtgaaa acctacgccc gcgccgaccg ccaataaact caagcccccg ctgatcgcga
     5281 gcggccacca ctcgaaacct ggatgcggcg gcagattccg cggcggatcg gccacgatga
     5341 tcggttgcag ccgctcgcgc agcgcccaag aaagacgcag ctgctctccc gctttggcgg
     5401 tcaccagcgc ggactgcggc tcgtagccct cgagctccgc acgcaccgct agaacccccg
     5461 gctccgcgaa aatctgccgg ggggtgactg ggctcggctc cagaacccga cccgccaccg
     5521 ataagaccgc gctcgccggt tcgacgtcca ggatcaacgt ggcgacatgc ttctgacact
     5581 cggcgagccg gtccgcgttc agctcgacca ggagcggatc cgcgtcgggg ggcatgtggg
     5641 acagcccaaa cgcgaagtgc tcggccgcgt cacgccaacg gccgagctca atctcgatca
     5701 accccaagtt gaccgaaacg tcagcagatc gccggtgctc ccaaacctga agcatgagat
     5761 tgaaggccgt ctgccagtcc tcttggtcca tggcgacctt ggccttgcgg taaattggat
     5821 tcaaatccag gctcgagccg ttgctcgacg tggcaccggc acccaaaatc gccggctcga
     5881 gcgcgtggct agtcgccgga actgcaaccg gtcccagaaa aacgctcgag cataacgtcg
     5941 cgatccatcg ccgcgcgccg gtcaggctcg gttttcggcc ttctttcgag caagtacgca
     6001 aggttcgcga ctacataaca gggccgcttc ccgagcacta gttttactag tcgacgccca
     6061 cgcgagtcgg tccggtgtgg ggctccgttg gcactcccgt tcgacgctgc cgacgcccgc
     6121 tggggttgaa cacaccgcgg gcccggctca gggtagccat ctgtccaaaa tcccgaaaag
     6181 gtctgcctcc gcgatgggtt tggccaggta atcgtccatg cccgcggcca agcaccgctc
     6241 ctggtcaccc ttcatcgcgc tcgccgtaag cgcaatgatc ggcacgcggc aacctgcctg
     6301 ccgaattgca gcggtcgctt cgagtccgtc gacctcgggc atctgccaat ccatcaggat
     6361 caatccgtag gaagctcgag ttgcggcttg gatggcttcg cgcccattcg gcacgatatc
     6421 cacgaagatc ccccgcttct cgaggaggcg agccaacaaa cgttgattca ccatgttgtc
     6481 ttccgcgacc agcacgcgcg cgcgatcccc agtggcggcg gctcgagaag gggcgagcgc
     6541 gcgcgacaag gaggtttgaa ccggacgtgg ctcctcggct atgaccaatg cttccgcccg
     6601 gctctcttgc aacccagaaa cgatgcggtg atcacgcacg ggcttggcga cacacacgaa
     6661 cacgccgcgt tcttgcgcac gcaatacttc gccacgcggc gtgccgctca ccagcaacat
     6721 gaaacgtgct gccttcagct tatcgatgcc gcgcaaggcg ccgcacgaac taacgccatc
     6781 cgagtccggc agcacctcgt cgaccagcac cgccaatatg ggcaaccccg tatcgatttg
     6841 acggaagcca tgaatcgcct ccgcagcgct ccggaagcca gagaacggaa gccccaagcg
     6901 ctgcgcggag ctggccatcg cggccagggt attttcgctc ccgctcacac cgcacaggcg
     6961 tcccacgagg cgattcgagt cgctcgcttc cccgcgttgc tccgcgggca aaccgaattt
     7021 gatctcgaac cagaactcgc tcccgttacc cggcacgctg ctgaagccga tctctccgcc
     7081 catcaactcg cagagtcgct tggagatggc gaggcctagg ccggtgccgc catacttgcg
     7141 ggtggtcgaa ccgtcggctt ggctgaacgc gcggaacagt cgaggctgga tctcgagcgg
     7201 gatgccgatc ccggtgtcgc gcaccgaaac ccggatcgtg agcagctcgt tgctgagcgc
     7261 cagcgcggag atcgcgacca gcacctcgcc gtagggcgtg aacttgatag cgttcgcgac
     7321 cagattcacc agaacttgcc ggaacctcag cggatccccg atcaccgtgc gcggcagctc
     7381 cgggtcgatc accccgttta gttcgatgcc cttctcccag gcttgctcgg ccaggatatc
     7441 caacgtatcg aacacccaat ccgcgaggtc gaacgcgatc gcctcgagca acatgcgacc
     7501 cgcttcgatc ttggagaaat ccaggatatc gttgatgatc gcgagtaggg ccgagccgga
     7561 ctttctgacc agggtcgcgt actctcgctg ctccggggat agcggcgtat ccaagagcaa
     7621 cccggtgagc ccaattacgc cgttgagcgg cgtgcgcacc tcgtggctca tggtggccaa
     7681 gaactcgctc ttggcgcgat tcgcgtcatc cgccgcatca cgagcgagcg ccatggccgc
     7741 cgcggcgtcc gcgagctctc gcgccttttg ctccaagccc gccgcggcac gcagccgctc
     7801 gatgccaatc gcgagtgcgt ccgcggctgc tcccagcgac gcccaggcgg tttgactcaa
     7861 agtctgccgg gagaatacgg cgaccacgcc aatcagacgc tcgcccaaca acatgggaaa
     7921 ccccgcgaac gctaccaagc cctctcgacg tgcccagtcc tgatcgggga tgcggctatc
     7981 tccgtaaacc gagtttagta ggaagggagt tctactctcg gcaatcgctc cgaccttcag
     8041 cgcgcccact tgaatccgtc cgtgcgaccc gtccgtgtgc gtgtagagcc cggcgctagc
     8101 ctgcatttcc agcaccttgc cgctcgcgtc gagcgtccaa attcgagcca aagcggcgcc
     8161 taggccctcc aacaagattc gggcacaacc ctcgagcacc gtgcgcatcg aatcggatgc
     8221 ggacagcgcg agcccgatgc gcgacgacat ttcgagcagc gctgcgcgtt cggcgagcgc
     8281 actctcctga gcacgtcgct cgctgatatc cgtgatgatg atcaccgaac cgcccgatcc
     8341 ttccgcggcc ctggctaaat tcaccatcga gaccagcgcg acgaactcgt ctccattctt
     8401 ctttacgagg cggagctcgc cgtcggctct gctgccccgc tccgccgcgc tcagtatcga
     8461 atcccaggtg gcccaccttc ccgtactatt ccgtgctggc agcggaggcc gctcgcgtga
     8521 ggacacaccg agctccgcga gcgggatgcc tcgtgctagc tcctgcgcag agtacccgag
     8581 cattctctga gccgccgggt tcatggtgcg caccgtgtat tgcgtgtcgg tcgagagaat
     8641 ggcgtagggt gcgccgtcca gaatagcgct ctgcagcgcg ttcaaacgcg atagtcgatc
     8701 ctgattctcg cggatcgtcc gcgtcatctc cgttgccaag cggacggagc gtgcgcgcgt
     8761 gcgcgcgagc acccaagtaa gagcgaaaga cagcagactg atgctgacgc caccgatcag
     8821 aatcagctcc gattcgctgc gcgccatccc cgactcgaaa cggggcgagc tcctgaaacg
     8881 ccaagtc
//
LOCUS       DASTKU010000411         7279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1862321, whole genome shotgun sequence.
ACCESSION   DASTKU010000411 DASTKU010000000
VERSION     DASTKU010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862321"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2041
                     /locus_tag="VFQ61_14120"
     CDS             <1..2041
                     /locus_tag="VFQ61_14120"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET9955645.1"
                     /translation="RHPGVLKVITTGNAPKLPGAKTKTNPNDRVLQLLQEEGVEYADQ
                     PVALVIADSLERAQEGAALVAISYDASDAKVDLAAAKGEGFAPKSLGPHGSTDSKRGD
                     PDAALAAAAARVDQTYQTPTQTHNPMEMHGLTVVWQGADHVTLYDTTQGIFGVRKKIA
                     TVFGIPPENVRVISKYVGGGFGCKGSPWSHVALAAMAARSVGRPARLVLTRQQMFSLV
                     GYRPHTIQHVVLGADRGGKLVAVKHEVWSETSRFDEFVEPCASVTRMLYACPNLQTSH
                     RLVRLDVPTPTFMRAPGESSGTFALETAMDELAYELKIDPVELRLRNYAERDEEEGKP
                     WSSKSLRECYRVAGQKFGWARRNANPRSLRQGRYLVGWGMASATYPARQSSASARARI
                     RKDGKLLVQAGSQDIGTGTYTIMAQIAADELGIPYEQVEFELGDTSLPETPVSGGSQT
                     ASSTGSAVKRAVSALKSKLVALAVADANSPLHGSKPEDVQAANGTLSVRNQAQKRETY
                     QALLARSAEPEISIEVKSEEKPERKQYSCHSFGAPFVEVRVDEELGEVRVTRVVAAYA
                     AGKILNQKTARSQYLGGIVWGIGFALQEHTVRDARSGRVVTRDLADYHVPVHADVPDI
                     DIIMVDEQDPYVNDIGAKGIGEIGITGVVAAIGNAVFHATGKRVRELPITLDKLI"
     gene            2093..3262
                     /locus_tag="VFQ61_14125"
     CDS             2093..3262
                     /locus_tag="VFQ61_14125"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET9955646.1"
                     /translation="MTELLRICEAGRKLASSDGRLLAADDERALWLATVVRVVGSGYR
                     RPGARLLFTRDRPVAGSVSGGCLEKDLIRTGPWLAARGPVVKTYDAREEDSAPVARTG
                     CGGSVDILIESLDPRLLESLGRALLEEARVLSITVHASRHRALRVGERMLFREHEAEP
                     VFETVHEPWFRREFARVSKEALRSGSGTCTWVSDSFDALVEVVEPPPHLFVFGLGEDV
                     RSVVELARTLGWNVSVAGPHVSVSARERFFGKAQLLEGPPSAVVQRLERCARPLALLM
                     SHDYERDKDALGALIGSRARYIGVLGPMRRTERLLTELEQSGRGVSPEMRSRVHGPAG
                     LHLGGDTAPEVALSIVAEAQAALWAASGVPLSLRDAAIHEPTPVVLTQAFEALGA"
     gene            3259..3894
                     /locus_tag="VFQ61_14130"
     CDS             3259..3894
                     /locus_tag="VFQ61_14130"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET9955647.1"
                     /translation="MSSTWACAVLAAGESRRLGSPKQLLLWQGESLLRRATRCALSCR
                     AGRVGLVLGAHRAAVSADVSGLEVEVLENSDYRQGIASSIRRAVTWAEQYSSRGLLLM
                     LCDQPRLTSSHLDALIDAADANGTLVCSRYEGQRGVPAVFPVRYFKALKRLRGDQGAR
                     VLLRGSDSGDADWARADASRAQRIVEIEWPEGAQDIDTVRDWRQLTGSSEI"
     gene            complement(3881..4531)
                     /locus_tag="VFQ61_14135"
     CDS             complement(3881..4531)
                     /locus_tag="VFQ61_14135"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9955648.1"
                     /translation="MKTSEANKKRTLRRSKLRARVGGRSARVVRDVLAAALQVFAERG
                     YAGLSMEEVAARANVNKTTVYRRWPTKSDLVGAALFTLRDEDPDPPDTGSLREDLFIT
                     LKLRAAQLGTAHRRAILNALLMSKSEPELQSLIEKLRRERPFLSRDLFERAIARQELP
                     PDANIELLSEALLGPLHHRVHWKQQTISDEFLRALVHLIVSGAQASSSPEASSKSR"
     gene            4678..5955
                     /locus_tag="VFQ61_14140"
     CDS             4678..5955
                     /locus_tag="VFQ61_14140"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET9955649.1"
                     /translation="MSSRSSRAALASQIVSDSDPPPSVSGPKPAASPRPSGKRRIVTM
                     GVVAIALLGGGSWWFAHRGLETTDDAQLDADVVAVPSRAGGTVVALHFKDNQRVDAGA
                     LLVELDPAQAQAKLAEAEAEVLAARASAEAADATAELVERKALADKNIARASLTGAAA
                     SVTATSDQVAEIQANLASATAQRDKAKLDFERAQRLVASGALASAELDAARTSLDSAQ
                     AAVDQASARMVTLQSSTSQARARVSEASARLGQAADTLPSQVVEAKARAAAARARVAT
                     SEAMRDLARLDLSYTKIYAPRAGIVSKRSVAVGQLVSAGQTVVMVVPGATRNGEVWVT
                     GNFKETQLAHMHVGQPATLTVDAYGQTLHGHVESFSGATGARFALLPPDNATGNFTKV
                     VQRVPVRVRLDDAPSELMMLPGMSVELTVNTRM"
     gene            5958..>7279
                     /locus_tag="VFQ61_14145"
     CDS             5958..>7279
                     /locus_tag="VFQ61_14145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET9955650.1"
                     /translation="MATAVSSAPVASVPVSSALSPKARRWLIAVSVALGALLEIVDTS
                     IVNVALPQMQASLGVTLSEAGWVITSYAIANVIILPLSAWLGDRFGKKRFFVFSLFAF
                     IAASILCGLSHSLGMLVFSRVLQGLFGGGLLAKAQSFLWETFPPEERGSAQALFGVVA
                     IAGPAIGPTLGGYIVTNLDWRWIFFINLPIGILAIFMAATFLPEDGARKATGSVDWLS
                     LAYLAVGLGALQTLLEEGYSESWFESSFISACAVTAAVCLVVFVYRQLNASQPVVDLR
                     VLRYRSLWAGCIVSAVIGMGLYGAMFAVPIFAQSVMRYTSQQTGMLMLPGALASAVAM
                     PLMARLLKRFDPRVLVAAGSLVVISALWMLSRLNPQTNEDELFWPLIIRGFGTVAMFL
                     PMSMATFASVPVQDVSKATGLYNLTRQLGGSIGIAVLTTVLSSRQAFHR"
BASE COUNT         1303 a         2159 c         2418 g         1399 t
ORIGIN      
        1 gcgccacccc ggcgtcttga aggtgatcac gacgggcaat gcacccaagt taccgggcgc
       61 aaagaccaag accaacccga acgaccgagt gctccaactc ctccaagagg agggagtcga
      121 gtacgcggat cagccagtag cactcgtaat cgcggattca ctggagcgag cgcaagaagg
      181 agcagcgctg gtcgcgatct cttacgacgc gagtgacgca aaagtggatc tcgccgccgc
      241 gaaaggtgaa ggttttgccc ccaagtcgct ggggcctcac ggctcgaccg actccaaacg
      301 cggtgatccg gacgcggcgt tggcagcggc cgcggcgcgc gtggaccaga cctatcagac
      361 gccgacgcaa acccataacc ccatggagat gcacgggctc accgtggttt ggcaaggggc
      421 agaccatgtg acgctctacg atacgaccca aggtatcttc ggagtacgca agaagatcgc
      481 caccgtgttt ggaatccctc ccgagaacgt gcgcgtcatc tccaagtacg tgggcggggg
      541 gttcggttgt aaggggtcgc catggtctca cgtggcgctc gcggcgatgg cggcgcgtag
      601 cgttgggcgg cccgcgcggc tggtgcttac ccgccagcag atgttcagct tggttggcta
      661 tcgtcctcat acaatccagc acgtggtgtt gggggccgac agaggcggca agctcgtagc
      721 cgtgaagcac gaggtttggt cggagacgtc tcgcttcgac gagttcgtcg agccttgcgc
      781 gagcgtgaca cggatgctgt acgcctgtcc caacctccag acttctcacc gattggtgcg
      841 cttggacgta cccaccccga cgttcatgcg agctccgggc gagtcatcgg gcacgttcgc
      901 gctggagacc gcgatggacg aactcgctta cgaactgaag atcgatccag tcgagttgcg
      961 gctccggaac tacgcagagc gagacgagga agagggcaaa ccctggtcca gcaagtcgct
     1021 ccgtgaatgt tatcgagtcg ctgggcagaa gttcggctgg gcgcggcgga acgcaaatcc
     1081 gcggtcactt cggcaaggca ggtacttggt cggttggggc atggccagcg cgacttaccc
     1141 tgcgcgccag agctctgcat cggcgcgagc gcgcattcgg aaagacggca agttgctggt
     1201 acaagccgga tctcaggaca ttggcacggg aacttacacg atcatggccc agatcgcggc
     1261 cgacgagctg gggattcctt acgagcaggt cgagttcgaa cttggggaca ccagcctccc
     1321 ggaaacaccg gtatccggcg gatcgcagac cgcatcgagt actggatcag cggtgaagcg
     1381 cgcggtctcg gcgctcaagt ccaagctcgt tgccttggcg gtggcggacg cgaattcacc
     1441 tttgcacggc agcaagccgg aggacgttca agccgcaaac ggtaccctga gcgtgcggaa
     1501 tcaagcgcaa aaacgtgaaa cctaccaggc cctgctggcg cggagcgccg aaccggagat
     1561 ttcaatcgaa gtaaagagtg aggaaaagcc ggagcgaaag cagtactcgt gtcactcgtt
     1621 cggcgcaccg ttcgttgagg tccgggtgga cgaggagctc ggtgaagtac gagttacccg
     1681 agtcgttgcc gcctacgccg cgggcaaaat cctgaatcag aagaccgcac gcagccagta
     1741 cctgggcgga atcgtgtggg gaatcggctt cgcattgcag gagcacacgg tgcgggacgc
     1801 gcgcagtgga cgggtggtga cgcgtgacct ggcggactat cacgttccgg tgcacgcaga
     1861 tgtgccagac atcgatatca tcatggttga cgagcaagac ccgtacgtca acgacatcgg
     1921 cgccaaggga atcggtgaga tcgggattac cggcgtcgtg gcggccatcg gcaatgcggt
     1981 tttccatgcc acgggcaagc gcgtacgtga actgcccatc acgctcgata agctgatttg
     2041 aaagtcggcg atgagaccgt cgtgacgaca tgaaccgatc ggggcgaacc ggatgactga
     2101 gcttctcagg atttgcgagg caggtcgtaa gctagctagc tcagatggcc ggcttctcgc
     2161 ggccgatgac gaacgggcgt tgtggctcgc aacggtcgtt cgagtcgtcg gctccggata
     2221 tcgaaggcca ggcgcgcggc tcctgttcac ccgggatcga cccgtggctg gctcggtcag
     2281 cggaggctgc ctggaaaagg acctgattcg gactgggccg tggctcgccg cacggggccc
     2341 cgtcgtcaaa acctatgacg ctcgggaaga ggactccgca ccggtcgcaa gaaccggctg
     2401 cgggggcagc gtggatatct tgattgagtc gttggatccg cggctgctcg agagcctcgg
     2461 acgggcgctg ctcgaagagg cccgcgtgct cagcatcacg gtccacgcga gtcgacatcg
     2521 ggctttgcgc gtgggagagc gcatgttgtt ccgggagcac gaggcggagc cggtattcga
     2581 gaccgtgcac gagccgtggt tccgccgcga attcgcccga gtgtcaaagg aggctctgcg
     2641 cagcgggtcg gggacgtgca cctgggtctc ggactctttc gacgctctag tcgaggtggt
     2701 ggagccgcct ccccacttgt tcgtgttcgg gctcggcgag gacgtcaggt cggtggtgga
     2761 gctagctcga acactggggt ggaacgtcag cgtggccgga ccgcatgtga gcgtttccgc
     2821 tcgggaacgc ttctttggaa aggcccagct cctcgaagga ccgccaagtg ccgtcgtaca
     2881 acggctggag cgttgcgctc gccctctcgc gctactcatg tcccacgact acgagcgaga
     2941 caaagacgcc ctgggggcgc tgatcggctc acgagctcgc tatatcggag tgctgggacc
     3001 catgcgcaga acggagcgtt tattgaccga actcgagcag tcgggtcgcg gtgtgagccc
     3061 cgagatgcgg tctcgcgtgc atggccccgc cggtttgcac ttgggtggcg acaccgctcc
     3121 cgaagtagcg ctctcgatag tggcggaggc gcaggcggcc ctgtgggcgg cgagcggtgt
     3181 cccactatcc ttgcgagatg cggccattca cgagcccact cccgtagtgc ttacgcaagc
     3241 gttcgaggcg ctcggggcat gagcagtacc tgggcgtgcg cggttttggc cgcgggggag
     3301 tctcgccgcc tgggctcacc caagcaactc ctgttgtggc agggcgagtc cctgttgcgt
     3361 cgggccacac gatgcgcgct ctcctgtcgc gccggccgag taggattggt gctgggcgca
     3421 catcgcgcgg cagtctcggc cgacgtatcg ggtctcgaag tcgaggtgct cgaaaactcg
     3481 gactatcgcc aaggcatcgc ctcgtccatt cgccgcgcag tgacttgggc cgagcaatac
     3541 tcgtcacgcg ggttgttgtt gatgctctgt gatcagcccc ggctcacgag ctctcatctc
     3601 gatgcgttga ttgatgcggc ggatgcgaac ggtacgttgg tgtgctcgcg ttacgagggt
     3661 cagcgtggcg tccccgccgt cttcccagtg cggtatttca aggcgttgaa gcgcttacga
     3721 ggagatcagg gcgcccgcgt gttgctaaga ggtagcgatt cgggagacgc cgattgggcc
     3781 agagcggacg cgagccgggc ccaaagaatc gtggagatcg aatggcccga aggggcccaa
     3841 gacatcgata cagttcgaga ttggcgccag cttacggggt ctagcgagat ttagagcttg
     3901 cttcgggcga gctcgaagcc tgcgcgccgg agactatgag gtggaccaga gcgcgcaaga
     3961 actcgtcaga aatggtttgt tgcttccaat gcacgcggtg gtgaaggggg ccgagcagcg
     4021 cttcactgag gagctcgata ttggcgtccg gcggtagctc ttgccgggcg atcgcgcgct
     4081 cgaacaagtc acgcgataaa aaaggccttt cccgtcgcaa tttctcgatc aaggactgga
     4141 gttcgggttc ggacttgctc atcagcaacg cattcaggat ggcgcgtcgg tgtgcggttc
     4201 cgagctgcgc cgcgcgcagc ttcaaggtaa tgaacaagtc ctcacgcaag gagccagtgt
     4261 caggaggatc gggatcctcg tcgcgcaaag tgaacaatgc ggcccccacc aggtccgact
     4321 tggtgggcca acggcggtac accgtggtct tgttgacgtt ggcgcgtgca gccacctctt
     4381 ccatcgaaag tccggcgtag ccccgttctg cgaagacctg aagcgctgcg gccagcacgt
     4441 ctcgcactac gcgcgcactt ctgccgccga cgcgggcccg cagcttggat cgcctaagtg
     4501 tgcgtttctt attggcttcg gaagtcttca cgttacttaa cgcaacaatc agtcgctttt
     4561 atagcgtcgg gtgctaacta aagcaaccaa ctgttgcgtt agagatctcg ccgggtccta
     4621 gctcgtcagc tgcgcagccc gcaacgtttc tgctggttcg cccagaagga atacagaatg
     4681 tcctctcgct catctcgagc cgcacttgct tcccaaatcg tctccgattc cgatccaccg
     4741 ccctcggtgt cgggaccaaa accggcagcg tcgcctcgcc cgagcggcaa gcgccggatc
     4801 gttaccatgg gggtggtcgc aatcgctctg ctgggagggg ggagttggtg gttcgctcac
     4861 cgaggcctgg aaacgaccga tgacgcgcag ctcgacgcgg acgtcgtagc agttccgagc
     4921 cgcgccgggg gaacggtggt ggcgctgcac ttcaaggaca accagcgtgt cgacgcggga
     4981 gcgctgctgg tcgagctgga tccggcgcaa gcccaggcca agctcgcgga agcggaagcg
     5041 gaagtgctgg ccgctcgcgc gtccgcagag gcggccgatg ccaccgcgga gctggtggag
     5101 aggaaagcgt tggcggacaa gaacatcgcg cgtgcctcgc tcacgggagc ggcggcatcc
     5161 gtgacggcta catcggacca agtcgcggaa atacaggcta atcttgcctc cgcaaccgct
     5221 caacgggaca aggccaagct cgatttcgaa cgcgcccagc gactggtcgc ttcaggggcc
     5281 ctggcgagcg cggagttaga cgccgcccgc actagcttgg acagcgcgca ggccgcggtc
     5341 gatcaagcct cggcgcgcat ggtcacgctg caaagctcga cgagtcaagc tcgcgctcgg
     5401 gtcagcgagg cttctgctcg gctggggcag gctgcggata cgttgccctc gcaagtcgtc
     5461 gaagcgaaag cgcgggccgc cgcggcgcgg gcgcgcgtgg ctacttcaga ggccatgcgc
     5521 gacttggctc gtttggatct gtcttacacg aagatctatg ctccgcgcgc gggcatcgtg
     5581 tccaagcgca gcgtagctgt cgggcagctc gtctccgcgg gccaaacggt ggtgatggtg
     5641 gtacccggtg cgactcgcaa cggcgaggtc tgggtgaccg ggaatttcaa ggaaacccag
     5701 ctcgctcaca tgcacgtggg tcagcccgcg actctcacgg tcgacgctta cgggcagacg
     5761 ttgcacggcc acgtcgagag cttctctggc gcaaccggcg cgcgctttgc gctattgccc
     5821 ccggataacg cgaccggcaa cttcacgaaa gtcgtgcagc gcgtgccggt gcgcgtgagg
     5881 ctggacgacg caccttcgga gctgatgatg ctgcccggaa tgtcggtcga gcttaccgtg
     5941 aatacccgga tgtagtcatg gctacagcag tctcttcggc gcccgtcgcc agcgttcccg
     6001 tgagcagcgc gttatctccg aaggcgcgtc gttggctcat cgcggtgagt gtcgcattgg
     6061 gggcattgct cgaaatcgtc gacacttcga tcgtgaacgt ggccttgccg cagatgcaag
     6121 cctcgctcgg ggtgactttg agcgaggcgg gctgggtgat cacgtcttac gccatcgcca
     6181 acgtcatcat cttgccgctc tcggcctggc tcggcgatcg cttcggcaag aagcgcttct
     6241 tcgtattctc tttgttcgcg ttcattgcgg catccatctt gtgcggtctc tcgcactcgc
     6301 tcgggatgct cgtcttctcg cgggtgctgc aggggctgtt cggtggcggg ctgctcgcca
     6361 aggcgcagtc tttcctttgg gagacatttc ccccggagga gcgcggtagc gcccaggcct
     6421 tgttcggtgt ggtcgccatc gccggaccgg caatcggccc caccctgggc ggctacatcg
     6481 tcacgaactt ggattggcgc tggatcttct tcatcaatct gccgattggc attctggcca
     6541 tcttcatggc cgccacgttt ttgccagagg atggcgcacg caaagccacc ggctcggtag
     6601 actggctctc actcgcgtat ctcgccgtcg gacttggcgc tctccagact ttgctcgaag
     6661 agggctattc ggagagctgg ttcgagtctt cctttatctc ggcctgtgcg gtgacggcgg
     6721 cggtttgtct cgtcgtattc gtctatcgcc agctgaacgc ctcccagccc gtggtcgact
     6781 tgcgggtgtt gcgttaccgc tcgttgtggg cgggctgcat cgtgtcggcc gtgatcggaa
     6841 tgggcttgta tggcgcgatg ttcgcggtcc ccatcttcgc tcagagcgtg atgcggtaca
     6901 cgtcccagca aacgggaatg ttgatgttac ccggtgcgct ggcatccgcg gttgcaatgc
     6961 cactcatggc gcgcctgctc aagcgcttcg acccacgagt gctcgtcgcc gcgggctcgt
     7021 tggtggttat ttctgcgctc tggatgctgt ctcggctcaa cccccaaacc aacgaggacg
     7081 agctgttttg gccgttgatc atccgcggct tcggcacggt ggcgatgttc ctgccgatga
     7141 gcatggctac ttttgccagc gttcccgtgc aggacgtttc caaggcgacg gggctataca
     7201 acctaactcg acagctcggt ggcagtatcg gtatcgcggt gctgacgacc gtgctgtcgt
     7261 ccaggcaggc ttttcatcg
//
LOCUS       DASTKU010000412         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1192370, whole genome shotgun sequence.
ACCESSION   DASTKU010000412 DASTKU010000000
VERSION     DASTKU010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192370"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1578
                     /locus_tag="VFQ61_14150"
     CDS             148..1578
                     /locus_tag="VFQ61_14150"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9955651.1"
                     /translation="MAELLTERPPMPVQQFELVVYLRQDSRVFLLTQGSSVGIGRSPD
                     NQIQIDDPSVSRHHARLRILDEVIVEDLGSANGTTLVRNARPRPDREDTDIRANQRLT
                     PGELASMTPGDVLRIGPVLLVLQKRGRSTPVEAPEPHGNPVNGGPAVLIDPEMRRVYE
                     LISRAAASDISVLILGETGAGKEVVAETVHRRSPRADKPFLRLNCAALPESLLEGELF
                     GHEKGAFTGAHSTKAGLIESTDGGTVFLDEIGELPLGTQAKLLRVLEERTVIRLGATK
                     PRRVNVRFVTATNRDLSREVRASRFRGDLYYRISGLVVRVPPLRKRRAEIEPLARHFL
                     KDFCARAGQAPPELAADAAELLYGYDWPGNVRELRNVMERAALLANGGQIKRDHVLLE
                     PALPELELEDADDFDAVTEVTKEKPQSLRPFSEEEAATGERDRVIRALESCGGNQTRA
                     AKLLGVSRRTLINRLEQFQLPRPKKG"
     gene            complement(1663..2709)
                     /locus_tag="VFQ61_14155"
     CDS             complement(1663..2709)
                     /locus_tag="VFQ61_14155"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9955652.1"
                     /translation="MSSFFSSSRAKFARMFAFGLGTSGLLAPVCALAAPPEASTQDAS
                     LKGGAAGERTEEEVRKAGEHYDRGLKLYTEGDHALAVIEFERAYRIVPDYRVLYNIGQ
                     VHIQLAHYAKARGALEQYVKEGGNQISADRRAAVQSDLEMLTARTATLRVQTNVPGAE
                     ILVDDSSVGVAPLDQPLLLDAGEHRVTVQMRGYETRTKQLTLAGRDNTELKVEIEKRA
                     EGQSSIIVQKVGAESDRTTWLWATWSATGAFAIASVTTELLGAKAASDLKNLRADPDA
                     TRSELDSASRRARTLLTVGDVLGGLAIASGGVALYMTLSSDKSEPEKDRAPVSKGRVA
                     LVVQPSYLGIRGSY"
     gene            complement(2706..>4265)
                     /locus_tag="VFQ61_14160"
     CDS             complement(2706..>4265)
                     /locus_tag="VFQ61_14160"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9955653.1"
                     /translation="PRIVAIKTALAEEAASEDFWKVLLDEARIASQIQHPNVCSIHAL
                     DRERGVVYLVMDYSDGGSLRELLDAEPSHRLQATFAARIVARVAAGLHAAHELVDQDG
                     SLLGVVHRDVSPQNILISAAGQVKIADFGVAKARGQLHAPTQTGEVKGKLSYMAPEQV
                     TTRDVDRRADVFALGCVLFEASTGERPFHGSDALATLYQLLEQPIKRPSELLPSYPPG
                     LEKILLRALDRDPDARYQTAEEMERALETWLASERAMVSEPDIAEVVRGALGAHIDAR
                     AESIRNAIQTLDTAPHHSVTPTVPTRPGIETLSGAASSTVPEAPATLPQPAPKRFPWA
                     VPLIAAAVIAGFVLVISRGKAPPVAAVSSAPLSAPKVEAAPVTPEAPRVRIAIRAQPE
                     EAAISIDDGPPVPSPYALEVQPEARVHRIRVSLAGYRERTEQIVFDASKEVAVLLEPE
                     ARPGRKAALRSAVVSSVRPNNPSVTNPEPAESPQPAPAPSRKVGELPTLTKKPPRVLD
                     SDNPFAAAEKP"
BASE COUNT          750 a         1370 c         1380 g          765 t
ORIGIN      
        1 atggtgaacg actcgtgcaa agcgctgaga tctccgccta atcctggtgg gcttctcgga
       61 caatgcgcgc tactctgagc acgggactgc gaccgctgta atccgcagcg ccaatcccag
      121 cagtacttgc aaccgagtaa acgtagaatg gcagaactac tcaccgagcg cccacccatg
      181 ccggtccagc agttcgagct cgtggtgtac ctgcggcagg attcgagggt attcctgctc
      241 acgcagggca gcagtgtcgg catcggacgc agcccagaca atcagatcca gatcgatgac
      301 ccttccgtct cgcgtcacca tgcgcggctc cgaattctgg acgaggtgat tgtcgaagat
      361 ttgggtagcg ccaatggcac gacgctggtg cgcaatgcgc ggccgcgccc agatcgcgaa
      421 gacaccgaca ttcgcgccaa tcagcggctc actccgggtg agctcgccag catgacgccg
      481 ggagatgtgc tccgcatcgg cccggtgttg ctcgttctgc agaagcgcgg tcgctcgacg
      541 ccggtggagg cgcccgagcc gcacggcaac ccggtgaacg ggggcccggc ggtgttgata
      601 gatcccgaga tgcgccgggt ttacgaactg atctcgcgtg cggccgcgag cgacatctcg
      661 gtattgatct tgggggaaac aggcgccggc aaagaggtgg tcgcggagac ggtgcaccga
      721 cgctcacctc gggccgacaa gccctttttg aggctgaact gtgcggcctt gccggagagt
      781 ttgttggagg gtgagctctt cggacacgag aagggcgcct tcaccggcgc ccactcgacg
      841 aaggctggat tgatcgagtc caccgacggg ggcacggtat tcttggacga aatcggggaa
      901 cttccgctcg ggacgcaggc caaactgctg cgcgttctcg aggaacgcac ggtcatacgt
      961 ctgggcgcca ccaagccgcg gcgcgtgaac gtccgattcg tcaccgcaac gaaccgcgac
     1021 ttgtcccgtg aggtgcgtgc ttctcgcttt cgaggcgacc tctattaccg aatcagcggt
     1081 ttggtcgtga gggtcccccc tctgcgcaag cgacgagcgg agatcgaacc cttggcgcgc
     1141 cacttcttga aggacttctg tgcccgtgcg ggccaagctc cgccggagct ggcggcggac
     1201 gcggcggagt tgctgtacgg ctacgattgg ccgggcaatg tacgcgagct acgcaacgtc
     1261 atggaacgcg cggcgctgct ggcaaacggt ggccagatca agcgtgacca cgtgctgctc
     1321 gagcctgcgt tgccggagct cgagctggag gacgcggatg acttcgacgc ggtcaccgag
     1381 gtgaccaaag agaagccgca aagcctgcgg ccgttctcgg aggaggaggc ggccacgggt
     1441 gagcgcgatc gagtaatccg cgcgctcgag agctgcggtg gcaatcagac gcgcgcggcc
     1501 aagctcctgg gggtgtcgag gcgcaccttg atcaaccgct tggaacagtt ccaacttccc
     1561 cgccccaaga agggctgaaa tctcggtgcg agcgaagaac ggtgagcgct tgctcaccgt
     1621 ctcttcagcc gccgttggcg tcgcttactc gcttgacctt gcctagtagc tgccgcgaat
     1681 cccgaggtaa ctaggctgca cgaccagcgc cacgcgaccc ttgctcaccg gcgcgcgatc
     1741 tttctccggc tcggacttgt cgctggagag ggtcatgtag agcgcgacgc caccgctcgc
     1801 gatggccaag ccgccaagga cgtctcccac tgtcagcaga gtgcgagcgc gacgcgaagc
     1861 cgagtccagc tcactgcgcg tagcgtcggg atctgctcgc aagttcttta ggtcgctcgc
     1921 cgcctttgcc cccaggagct cggttgtcac cgaggcgatc gcgaaggcac ccgtcgccga
     1981 ccaagtcgcc cagagccacg tggtccgatc gctctccgcg ccgacctttt gtacgatgat
     2041 tgagctctgg ccttcggctc gcttctcgat ctcgaccttg agctccgtgt tgtctcgacc
     2101 ggccagcgtg agctgcttcg tgcgcgtctc gtagccgcgc atctgcacgg tgacacgatg
     2161 ttcacccgcg tcgagcagca atggttgatc gagcggagcc acacccacgg agctatcatc
     2221 caccaggatt tcggctccgg gcacgttggt ttgcacacgc agagtcgcgg tgcgcgcggt
     2281 cagcatttcc aaatctgact gcaccgccgc gcggcgatcg gcggagatct ggttgccgcc
     2341 ctctttgaca tactgctcga gcgcaccgcg cgccttggcg tagtgcgcga gctggatgtg
     2401 cacttgcccg atgttgtaca gcacgcggta gtcggggacg atccggtagg cgcgttcgaa
     2461 ctcgatgacc gccagcgcgt gatcgccttc ggtgtagagc ttcaggccgc gatcgtagtg
     2521 ttcgccagct ttgcgaactt cttcttcggt gcgttcgccc gcagcgcccc ccttcaagga
     2581 ggcgtcctga gtcgaggctt cgggtggcgc ggcgagcgcg catactgggg cgagtagccc
     2641 gctcgttccc agtccgaatg cgaacatgcg agcgaacttg gcgcggctcg acgagaaaaa
     2701 cgaactcatg gtttctccgc agcggcgaaa ggattgtccg aatccagaac acgcggcggc
     2761 ttcttagtca acgtcggcaa ctcacctacc ttgcggctcg gcgcgggagc aggctggggc
     2821 gactccgctg gctcagggtt ggtcacgctc gggttgttgg gccgcacact gctcacaacg
     2881 gccgatcgca gcgccgcctt gcgtccagga cgcgcttccg gctcgagcaa gaccgcaact
     2941 tctttcgacg cgtcgaacac gatttgttcc gtgcgttcac gataacccgc caagctgacg
     3001 cgaatccggt gcactcgcgc ctccggctgg acctccaacg cataggggct tgggacaggg
     3061 ggaccgtcgt cgatcgagat cgccgcttcc tcgggttgag cccggatcgc gatacgcacg
     3121 cgcggcgctt ccggagtgac cggcgcagct tcgaccttcg gcgcgctcaa aggagcagag
     3181 ctgacggccg caacgggcgg tgccttccca cgcgagatca ccagcacgaa gccggcgatc
     3241 accgcggcgg caatgagggg caccgcccaa ggaaagcgct tgggtgccgg ttgcggcaac
     3301 gtcgccggtg cctcgggcac ggtactcgac gctgcccccg acaaagtctc gatgcccggg
     3361 cgcgtcggta cagtcggggt gacggaatga tgaggtgccg tgtccagcgt ttggatggcg
     3421 ttgcgaatgc tctcggcgcg cgcgtcgatg tgggccccga gcgcaccgcg caccacttcc
     3481 gcgatgtccg gttccgaaac cattgcgcgc tccgaagcca accaggtctc gagcgcgcgc
     3541 tccatctcct ccgcggtctg atagcgggcg tcgggatcgc gatcgagagc gcgcagcaga
     3601 atcttctcca acccgggcgg gtaactcggc aacaactcgc tgggccgctt gatcggctgc
     3661 tctaacagtt gatagagcgt cgcgagcgcg tcgctcccat gaaacggccg ctcaccggta
     3721 ctggcttcga acagcacgca accgagcgcg aacacgtcgg cgcgccgatc gacgtcacgg
     3781 gtcgtgactt gctccggggc catgtaggag agcttgccct tcacttcacc cgtctgagta
     3841 ggcgcgtgca actgcccgcg cgccttggcc acgccgaagt ctgcgatctt cacctgaccc
     3901 gccgcggata tcaggatgtt ctgcggcgag acgtcgcgat gcaccacccc gagcaacgag
     3961 ccgtcttgat cgaccaactc gtgcgcggcg tgaagaccgg ccgcgacgcg cgctacgatg
     4021 cgcgccgcaa acgtggcctg caggcggtgt gagggctccg cgtccagcag ctctctgagt
     4081 gagccaccgt ccgagtagtc catcaccagg tagaccactc cgcgctcgcg atccaacgca
     4141 tggatcgagc acacattggg gtgctggatc tgcgaagcaa tcctagcttc gtcgaggagt
     4201 accttccaga agtcttcgct ggctgcctct tcggcgagcg cggtcttgat ggctacgata
     4261 cgagg
//
LOCUS       DASTKU010000413         4101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1192372, whole genome shotgun sequence.
ACCESSION   DASTKU010000413 DASTKU010000000
VERSION     DASTKU010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192372"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFQ61_14165"
     CDS             <1..355
                     /locus_tag="VFQ61_14165"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9955654.1"
                     /translation="NDDNLPNKSIPTVDELPKNARRHGLLTCTSDAAVTPIEVVDSVV
                     LGTDPDAATVELSGPDVAPQHARIWSTAAGAFLLEDLGSASGTFVNEVRVQRRTLMLG
                     DRIRLGATLVFEFTF"
     gene            complement(517..1545)
                     /locus_tag="VFQ61_14170"
     CDS             complement(517..1545)
                     /locus_tag="VFQ61_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955655.1"
                     /translation="MTWSRAAGAQTLAPSVEAGGGPAVTAGALDPETRVLAREFAIRG
                     AEAYDAGDFEAALENFNRATAILDVPSIAVMQARTLVKLGRWLEGLDRFQQTARLDLD
                     ATAPLPYREAVAQAREEAEALRAQVPQVALLLGSDTESSGAELSVSFDEKPVPVALLN
                     ISRPVNPGSHTVRVSSAGRVFFERTLDVAPSQQIQVHIPPAPSAEAPSPAARRATPVT
                     GMSQTPSHGGAPHDEPRSVNWPLYGALGVAGLGFATAVTTAVLGSNHKATLDAACDSE
                     TNTCPPEYEDEISALNTERSLFYLSAGVTALAGGVAAYLWLTEEGDEAGAVALRLSPS
                     TLSFVGHY"
     gene            complement(1625..3010)
                     /locus_tag="VFQ61_14175"
     CDS             complement(1625..3010)
                     /locus_tag="VFQ61_14175"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9955656.1"
                     /translation="MSSMTQRRLRPVTASPESSPDVARVSPYLAGEVIGDRYRLDREI
                     GRGGMGVVWVAHSLVLGVDVALKLIHAETAGPGLATRMAREAHAAARLGHPALVRVFD
                     FGWTNRGDPFLVMEFVQGETLASKIQRDGPMPAIKAVQALLPIADGLRLAHERSIVHR
                     DIKPDNILLATDALGRIQPKLLDFGIAKVDHAPVNGKLTQMGAVLGSPEYMSPEQAQG
                     LEGVDARSDVWSLSVALYELLTGHVPFTGPNYNALMQSIIHEPAQPPQGGDSELWQVV
                     AKGLAKLPERRWASMSEFGEALALWLYDHGVKEDLSGNSIRAVWLDRPSLLQAAPSTG
                     APPPLNRRKTRGFEQTVERVGPNLLRNPTVRWVAGVLAVSALVFVGLDVFVRKEGGPA
                     PATVEAHAVGPSVAPLATLLEPSDPTQTPELGRARPEAAAPGPNEQPAPAKRNSRAPI
                     KTKKPKHDFGF"
     gene            complement(3293..>4101)
                     /locus_tag="VFQ61_14180"
     CDS             complement(3293..>4101)
                     /locus_tag="VFQ61_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955657.1"
                     /translation="GNLFPFASGSAIASRAENYGHLLLWPDGQSYRVLPMGSLRTLLT
                     EGRPDVTPLIHLDPEPHAGPDTLGRPTQVWTFETSRGKLHLHQAQVEESELGGPLLCR
                     FLLEWLSVAPSSTVCEQGLLPTRAEFESPRGAKLVWDTSELEVESNAEVPKLAVPPRD
                     ALFRQYGVPEPPPVLSPAELGLLRKAGKTGTLTAHNPTTQLQWLLLDGAPVGRIPPRS
                     AWTTAGLSQGTYFARLVDFFGVEAGNQPGFLAGDEATFGAPSSPAYEPEQ"
BASE COUNT          692 a         1313 c         1370 g          726 t
ORIGIN      
        1 caacgacgat aacctcccca acaagtcgat acctacggtc gacgaactgc cgaagaatgc
       61 ccgccgacac gggctgctca cgtgtacttc ggacgcggca gtcacaccta tcgaagtggt
      121 ggactcggtg gtgctgggca cggaccctga cgcggcgacg gtcgagctct ccggcccgga
      181 tgtcgcaccg caacatgcga ggatctggag caccgccgct ggggcatttt tgctcgaaga
      241 ccttggctcg gccagcggta cgttcgttaa cgaggtgcgc gtgcaacggc ggacgctcat
      301 gctcggggac cgaattcggc tcggtgcgac gctggtcttc gagttcacct tctagttaac
      361 tggtactttt tgacgatggc cgcccggctt ggaccgggga ttgcccaggg cggctggcgg
      421 tgggtctcgc gcgcttgtct cgccgaccag cgggccgcaa atccgggcct tgtcccacct
      481 ggcgtgggac aagcgtgact catttggggt gggtcgctag taatgtccca cgaagctcag
      541 ggtcgacggt gagaggcgca gcgcgactgc tccggcctcg tccccctcct cggtgagcca
      601 caaataggcg gctacacccc ctgccaaggc ggtgaccccg gcgctcaagt agaacagcga
      661 ccgttccgtg ttcaaggcgc tgatttcgtc ctcgtattcg ggtgggcagg tgttggtctc
      721 ggaatcacag gctgcgtcta gcgtggcctt gtggttgctg ccgagcaccg ccgtcgtcac
      781 cgctgtggcg aagccgaggc cagcgacgcc caacgcgccg tagagcggcc agttgacgct
      841 cctcggctcg tcgtgcggcg cgccgccgtg cgatggcgtt tggctcatgc ccgtcactgg
      901 cgtggctctg cgtgctgcag gagagggggc ttcggcgctc ggggcgggtg gtatgtgcac
      961 ctgaatttgc tggctcggcg cgacgtcgag cgttcgctcg aagaacaccc gacccgcgct
     1021 ggacacccgg accgtgtgcg aacccgggtt aacagggcgc gagatgttca acaaggccac
     1081 aggcacgggt ttttcgtcaa agctgacgct gagctccgcg cccgacgact cggtgtcgct
     1141 gcccaagagt aacgccacct ggggaacctg ggcacgcaac gcctcggctt cctcccgggc
     1201 ctgggcgacg gcctctcggt atggaagcgg cgctgtcgcg tcgaggtcga ggcgcgcggt
     1261 ttgttggaag cggtccaagc cctcgagcca gcgtcccaac ttcacgagtg tgcgcgcctg
     1321 catcacggcg atcgaaggca cgtcgaggat cgccgtcgcg cgattgaagt tctcgagcgc
     1381 ggcctcgaag tctccagcgt cgtaggcctc tgcgccgcga atggcgaact cgcgagcgag
     1441 cacgcgcgtc tctggatcga gggctcccgc ggtcaccgct ggtccgccgc ccgcttccac
     1501 gctcggggcc agcgtctgcg ctccggccgc gcgcgaccac gtcatcgcca agcacgcgac
     1561 ggcgccagcc agcgaaagta cccgaggtgg ctcgagccgt ctcaccgtac gccgcgcatt
     1621 tcccctagaa gccgaaatca tgcttgggtt ttttggtttt gatgggggct cggctgttgc
     1681 gcttggcagg tgcgggttgt tcatttggcc ccggcgctgc agcctcgggc cgagctcgac
     1741 cgagctctgg agtctgggtg gggtcgctcg gttcgaggag cgtcgccaag ggagccaccg
     1801 atgggccgac ggcatgggct tcgaccgtcg cgggtgcagg gcccccctcc ttgcgtacga
     1861 acacgtcgag accaacgaaa acgagcgcac tgacggccaa gactccggcg acccaacgca
     1921 ccgtcgggtt acgcagcagg ttgggtccga cgcgttcgac cgtctgttcg aagcctcggg
     1981 ttttgcggcg attcaacggg ggcggcgcgc cagtactcgg tgccgcctgc aacaggctcg
     2041 gacggtcgag ccaaacggcg cggatcgagt taccgctgag gtcttccttg actccgtggt
     2101 cgtagagcca cagcgcgagc gcctcaccga actcactcat cgaagcccaa cgtcgttcgg
     2161 gcagtttggc cagacctttg gccacgacct gccacagctc ggagtcaccg ccttgcggtg
     2221 gctgagctgg ctcgtgaatg atcgattgca tcaacgcatt gtagttgggc ccggtgaacg
     2281 gcacatggcc ggtcaagagc tcgtacaacg caacactcaa ggaccagacg tcgctgcggg
     2341 cgtctactcc ttctagacct tgggcctgtt ccggagacat gtactcgggg ctgcccaaca
     2401 ccgcacccat ttgcgtgagt ttgccgttca ccggcgcgtg gtcgaccttt gcgatgccga
     2461 agtcgagtag cttcggttgg atgcgcccaa gggcgtctgt cgcgagcaag atgttgtccg
     2521 gcttgatgtc gcggtgaacg atgctgcgct cgtgagccaa tcgcagcccg tccgcgatcg
     2581 gcagcaacgc ttgaaccgct ttgatggcag gcatcgggcc gtcgcgctgg atcttcgagg
     2641 ccaacgtctc accctgcacg aactccatca ccagaaacgg gtcgccgcgg ttggtccaac
     2701 cgaagtcgaa gacgcgcacc agggcggggt gtccgagccg ggcggcggcg tgggcttctc
     2761 gcgccatgcg cgtggccaag ccgggccccg ccgtttcggc gtggatgagt ttgagcgcca
     2821 cgtcgacccc caagacgagc gaatgcgcga cccagaccac acccatgccg ccgcgcccaa
     2881 tctcgcggtc tagccggtac cgatcgccga tcacctcacc cgccaggtag gggctgacgc
     2941 gcgctacgtc ggggctggac tccggcgaag cggtaacagg gcgaagccga cgttgcgtca
     3001 tggagctcat agaaacggcc gaccagagtg agcgttggat ctcactttag gcggaaacgg
     3061 tcagagcgtg agcgcggaac agattttctc gaggcgctag tccaccgagt acaggagcga
     3121 ccgaactttg tgcctgatcc aggtgccgac gtccagccta ggaaacggtt ccactccgac
     3181 tgtggtccaa gcggattcaa gggcgtttca gcccgttcgg aatcgaaacg cacactcggg
     3241 acccgcactt atgaaagtgc gagctgaggc ccgacgggat agctcgactc aactactgtt
     3301 cgggctcgta agcgggggag cttggggccc cgaacgtggc ttcgtctccc gcgaggaagc
     3361 cgggttggtt gcccgcctcg actccgaaga agtccaccaa gcgggcgaaa tacgtgcctt
     3421 ggctgaggcc tgccgtcgtc cacgccgagc gtggagggat acgtcccacg ggcgcgccgt
     3481 cgaggagcag ccactgcagt tgcgtggtgg ggttgtgggc ggtcaacgtg cccgtcttgc
     3541 cggctttgcg taacaagccg agctccgcgg ggctgagtac cggtggcggc tcggggaccc
     3601 cgtattgcct gaagagggcg tcccgcggcg ggacggcgag cttgggcacc tccgcgttcg
     3661 actcgacctc gagctcgctc gtgtcccaca ccagcttggc tccgcgcggc gactcgaact
     3721 cggcgcgcgt cggcaagaga ccctgttcac acaccgtcga ggagggcgcc acggacaacc
     3781 actccagcag aaagcgacaa agcaacggcc cgccaagttc actctcctcg acttgggctt
     3841 ggtgcagatg gagtttgccg cggctggtct cgaacgtcca aacttgcgtc gggcgcccaa
     3901 gcgtgtcggg ccccgcgtgt ggctccgggt ccaaatgaat gagcggcgtg acgtcggggc
     3961 gcccctcggt gagcagcgtc cgaagactgc ccatcggcaa cacgcgatag ctctgcccat
     4021 cgggccacag caacaagtgc ccgtagtttt cggcgcgtga cgcgatggcc gagccgctcg
     4081 cgaatggaaa tagattgccg t
//
LOCUS       DASTKU010000414         4879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_523176, whole genome shotgun sequence.
ACCESSION   DASTKU010000414 DASTKU010000000
VERSION     DASTKU010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523176"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="VFQ61_14185"
     CDS             <1..1356
                     /locus_tag="VFQ61_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955658.1"
                     /translation="GAFELFGWEVGVARSVVLAFSALLVFAVYDCTRLLASRTAGPSA
                     AHAGGAVSLAFLLLASSFAGLRVSVMIGIPALALAAFAFWAALRSQDSPERRRAWVAL
                     AGMAFAASLSTKLFTAFLLPLLLGCILQPELRTVTAVARRAKTLGALARGCALPLLFF
                     AAGFALMLSLTMAPAMRAEAWSQLYLTHAKAEGAYKSQYTVGVLLSRDWLLYVMAGLG
                     SLALIAVRGARIGLWFSAWLGLGALVLSRHTPLWAHHNQLMIVPAAVLAGCAVALVPA
                     LVPWSQFAARSVGFARSGAVLTGVALALAGLFAVSGERAELLLQPYGRSDAGDRAALK
                     KLQELGVRADGYMVSSRQIYAFYLGVPVPPTLSVTSAKRFRSGHLDASILNAEIRRHR
                     ARVVVMSQRWPKPVRDSVRRSLGAEYELIYQDRKNQDLAVYRLRDDAASGRPSLTAEA
                     R"
     gene            1382..1816
                     /locus_tag="VFQ61_14190"
     CDS             1382..1816
                     /locus_tag="VFQ61_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955659.1"
                     /translation="MFEQTERQFFAGTDVNPVITALCGGLQTQGFRGQQTAANTWQAQ
                     SLATSYGTSLRVTMWVVPSPSGFAVEARYSAEFENNALIVFAVLWFVFFPAAIVLAIL
                     GYQDVTNRLFTLRGSFWMPVASRIVAPNFGPPLGPGPMPPAA"
     gene            complement(1838..2293)
                     /locus_tag="VFQ61_14195"
     CDS             complement(1838..2293)
                     /locus_tag="VFQ61_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955660.1"
                     /translation="MRERPSNGAGDGPGSEELARVDDEALLCALVLAPSTFSRNRFFG
                     LFEGAERKRLRRRAARVRGIIRQLVHPERGQAEIVGERVLNDGRVLLRYTLDQLGYMR
                     TASLSPLEAATLRFALHRAGRGELPEEDRKLVETSLARLSGGLSVALEG"
     gene            complement(2277..3371)
                     /gene="prfA"
                     /locus_tag="VFQ61_14200"
     CDS             complement(2277..3371)
                     /gene="prfA"
                     /locus_tag="VFQ61_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET9955661.1"
                     /translation="MLPRAKLNSLVVRHAEVERLLCEPQVLSDPQRMNALNRERAQLV
                     PVVDAMRELEDIERRVREDKEVLDDPELGALARQELTELEAQQNTLEQQIRVLLLPKD
                     PNDEKNTILEIRAGTGGEEAALFAADLFRMYARYAEKRGWRVEILSSSEASAGGLKEI
                     IAGVTGQRVYSSLKHESGVHRVQRVPSTEAQGRIHTSTATVAVLPEADDVDFVLDEKD
                     LKIDIAASGGPGGQGVNTTNSAVQITHVPTGMIVKCQDERSQIKNKAKALKILRSRLL
                     EIEREKQAEAQRDERRGMVKGGDRSEKIRTYNFPQNRLSDHRIGLTLYELDRIIEGDL
                     DQLISALSAFHQAALLEASQADSAIYAGAP"
     gene            complement(3404..4564)
                     /locus_tag="VFQ61_14205"
     CDS             complement(3404..4564)
                     /locus_tag="VFQ61_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HET9955662.1"
                     /translation="MSNQPAVIGPDGVARPYIGGQALLEGVMMRSPHAFAAVVRRKNG
                     ELVVREEPMNDPRRGVRAWPLVRGVTALVEALRLGSRSLRFAAEIYEQDLEEEEARAG
                     TQSSPDAVRPPAAKPEQPSILSLLSLPIVALAMQDGSTPVSPKPKKEGSSALTFLSVA
                     FALGLFIALPQASAAGISALFKLDLDVRSAAFQALTGAAKLIIIIGYMLAIRRIPEIR
                     RVFQYHGAEHKSIYAYESGMPLSVENARSKTTLHPRCGTTFIVMVALVSVFVFSALGP
                     LLPQFGLGRVADNVLFFLLKLPFLPVIAAATFELQRFLARFGTRGPLSVLLWPGFLVQ
                     KITTIEPDDKQLEVALGALAVTLAREQGRVAGAEPTSDRTYPSYATLLAGHS"
BASE COUNT          716 a         1592 c         1584 g          987 t
ORIGIN      
        1 ggagccttcg agctcttcgg gtgggaggtt ggagtcgctc ggagcgtcgt cctggcgttc
       61 tcggctctgc tcgtgtttgc cgtctacgac tgcacgcgcc tgctcgcatc cagaaccgcg
      121 gggccatcgg ccgcgcacgc cggaggcgca gtctctttgg ccttcctgtt gctcgcctcg
      181 agcttcgcgg gcctgcgcgt cagcgtgatg attgggatcc ccgcgctggc tttggccgcg
      241 ttcgcgttct gggccgcgct tcggagccaa gacagccccg agcggcgtcg cgcttgggtt
      301 gcgttggccg ggatggcgtt cgcggcctct ttatcgacca agcttttcac tgccttcctg
      361 cttccgctgc tgcttggttg cattctgcag ccagagctac ggaccgtgac cgcggttgcc
      421 cggcgagcca agaccctcgg cgcgctcgca cggggctgcg cccttccgct cttgttcttc
      481 gcggctggct tcgctctgat gctgagcctc accatggcgc ccgccatgcg agccgaggcc
      541 tggtcgcaac tgtacctgac gcacgcaaaa gccgaagggg cctacaagtc gcagtacacg
      601 gttggagtac ttctttcgcg tgattggctg ctctacgtga tggcggggct cggttcgttg
      661 gcgctgatcg ctgttcgcgg ggcacgcatc gggctgtggt tctctgcctg gctcggcttg
      721 ggcgcgctgg tgctctcgcg gcacacgccg ctttgggcgc accacaatca gctaatgatc
      781 gtgcccgcgg ccgtgctcgc cgggtgtgcg gtcgcgctag tgccggcgct ggttccctgg
      841 tcgcagttcg cagctcggag cgtgggattt gcccgttcgg gcgcggtgct aacgggagtc
      901 gcgctggctc ttgcgggcct ctttgcggtg tcgggagagc gtgccgagtt gttgctgcag
      961 ccctacgggc gaagcgatgc gggcgaccgc gccgcattga agaagctgca agagctcggg
     1021 gtgcgtgcgg acggatacat ggtgagctcg cgccagattt acgctttcta cctgggcgtg
     1081 ccggtaccgc cgacgctctc ggtgacctcg gcgaagcgat ttcgctctgg gcacttggat
     1141 gcttccattt tgaacgccga gatccgtcgt catcgagccc gcgtggtcgt gatgtcccaa
     1201 cgctggccga aacccgtgcg cgactcggtc cgccgctcgc tcggagcgga atacgagctc
     1261 atctaccaag accgaaagaa ccaagacttg gccgtctatc gcttgcggga tgacgctgct
     1321 tccgggcgcc cgtcattgac cgcagaagcg cggtgacgcg ctctggatta ggcttcgtcc
     1381 cgtgttcgag cagaccgagc ggcagttctt tgcgggtacc gacgtcaacc cggtgatcac
     1441 cgcgttgtgc ggcgggttgc agacgcaggg gttcagaggc cagcagaccg cggccaatac
     1501 ttggcaggcc cagtccctgg ccaccagcta cgggacgtcg ctgcgcgtga cgatgtgggt
     1561 cgttccctcg cccagcggtt tcgccgtgga agcgcggtat tcggccgaat tcgagaacaa
     1621 cgcgctgatc gtgttcgcgg tgctttggtt cgtattcttc ccggccgcaa tcgtgctcgc
     1681 gatcctggga tatcaggacg tgaccaatcg cttgttcacg ctgcgaggat cgttctggat
     1741 gccggttgcc tcgcgcatcg tcgcgcccaa tttcggtcct ccgcttgggc cgggcccgat
     1801 gccgcctgct gcctagcggc ttcaagcgtc gaacggctca accctcgagc gcgacagaga
     1861 ggccgccgct gagacgcgcg aggctcgtct cgaccagctt gcgatcttcc tcgggcaact
     1921 cgccgcgccc ggcccggtgc agcgcgaagc ggagcgtggc agcttcgagc ggcgagagcg
     1981 aggctgtgcg catgtacccc agctgatcga gggtgtaacg aagcagcact cgaccgtcgt
     2041 tcaatactcg ctcccctacg atctcggctt ggccccgttc cggatgtacc agctggcgaa
     2101 tgatgccacg cacacgggcg gcgcggcgcc gcaaccgctt gcgctccgcc ccttcgaaca
     2161 agccgaagaa gcggttacgc gagaacgtcg aaggcgccag caccaacgcg cagagcaacg
     2221 cctcgtcgtc cactcgtgcg agctcctccg agcccggccc gtcaccggct ccgttgctag
     2281 ggcgctcccg catagatcgc cgagtctgct tggctcgctt cgagcagcgc cgcttggtgg
     2341 aacgccgaga gcgcagagat caactgatcc aagtcgcctt cgatgatgcg atcgagctcg
     2401 tacagcgtga gcccgatgcg gtgatcactc agccgattct gcgggaaatt gtaggtgcgg
     2461 atcttctccg agcgatcgcc gcccttgacc atgccgcgcc gctcgtctcg ctgcgcttcg
     2521 gcctgcttct cgcgttcgat ctcgagtagc cggctgcgca agatcttgag cgctttggcc
     2581 ttgttcttga tttggctgcg ctcgtcctga cacttgacga tcatgcccgt aggcacgtgg
     2641 gtgatctgaa ccgcgctgtt cgtggtgttc acgccttgcc cgccgggacc gcccgaagcg
     2701 gcgatgtcga tcttgagatc tttctcgtcg agtacgaagt cgacgtcgtc tgcttccggg
     2761 agcaccgcga ccgtggccgt agacgtgtgg attcggcctt gcgcttcggt gctgggcacg
     2821 cgctgcacgc gatgcacgcc gctctcgtgc ttcaggctcg agtacacgcg ctgcccggtc
     2881 acgccggcga tgatctcctt gagcccgccc gcggaggcct cgctgctgct caggatctcg
     2941 acgcgccagc cgcgcttctc cgcgtagcgc gcgtacatcc ggaacaaatc ggcggcgaac
     3001 agcgccgctt cttcgccgcc ggtcccggcg cggatttcga gaatggtgtt cttctcgtcg
     3061 ttgggatctt tgggcaggag caacactcgg atctgttgct ccaacgtgtt ttgctgagct
     3121 tcgagctcgg tgagctcttg ccgcgcgagc gccccgagct ccggatcgtc cagcacttct
     3181 ttgtcttcgc ggactcgtcg ctcgatgtcc tcgagctccc gcatggcgtc gaccactggc
     3241 accagctgcg ctcgctcgcg gttcagcgcg ttcatacgct ggggatccga gaggacttgc
     3301 ggctcacaca acaggcgctc cacctcggca tggcgcacca ccaggctatt cagtttcgct
     3361 ctcggcagca tctttgcggc tcaatccctc gaacgtgccc ggctcaactg tggccggcga
     3421 gcaacgtagc gtagctcggg taagtgcggt cgctggttgg ctccgctccg gcaacgcggc
     3481 cttgttcacg ggccaacgtt acggcgagcg ctcccaacgc cacttcgagc tgcttgtcat
     3541 ccggttcgat ggtggtgatc ttctgcacca agaaacccgg ccagagcaag acactcagcg
     3601 gaccgcgtgt accgaagcgg gcgaggaagc gttggagctc gaacgtggcc gccgcgatta
     3661 ccggcaaaaa cggcagcttg agcaagaaga acaacacgtt gtccgccacc cgcccgaggc
     3721 cgaactgtgg tagcagcggg ccgagcgcgc tgaacacgaa cacggagacc agcgccacca
     3781 ttacgatgaa cgtcgtgccg caacgcgggt ggagcgtcgt cttgctgcgc gcattctcga
     3841 cgctcagggg catgcccgac tcgtaagcgt aaatcgattt gtgctcggcc ccgtggtact
     3901 gaaacactcg ccggatttcg gggatgcgcc gaatcgccag catgtagccg atgatgatga
     3961 tgagcttggc cgctcccgtc agcgcttgaa aagccgcgct gcgcacgtcg aggtcgagct
     4021 tgaacaacgc gctgatcccg gccgcgctgg cctgtggcag cgcgatgaac aagcccagcg
     4081 cgaacgcgac gctcaggaac gtgagcgcgc tcgacccctc cttcttgggc ttggggctca
     4141 cgggcgtgga gccatcttgc attgcgagcg cgacgatagg cagggaaagc aggctcaaga
     4201 tcgacggctg ttcgggtttc gcggcggggg gcctgaccgc gtctgggctc gactgagtgc
     4261 cggcgcgcgc ttcttcctct tccagatctt gctcgtagat ctcggccgcg aaacgtaacg
     4321 aacggctccc gaggcgcagc gcctccacca gcgcagtcac gccgcgaacc agcggccacg
     4381 cgcgcacgcc gcggcgtgga tcgttcattg gctcttcgcg cacgacgagc tcgccatttt
     4441 tgcgccgaac caccgcagca aacgcgtgcg gcgagcgcat cattacgcct tcgagcaacg
     4501 cttgcccgcc gatgtaaggt cgggcaacgc cgtccggacc gatcactgcc ggttggtttg
     4561 acatctgccg gagtatagcc cgagtgtcgc ggtgcgctct gcttcccgcc gcgcagtgtt
     4621 gcgtttcccg cgccgctgcg tttcccgcgc cgctgcgttt cccgcgccgc cggcgccgtg
     4681 gttgccgcgt tgttggcggt ctctccgcgg agtttctttg gcgccctgtc tctctggcgc
     4741 cctgtttctt agcgccgttc tctcggtgat ttgcgccgac gtctcggttt tgtgactacg
     4801 acgtgcagtg gggaggggtc gccgttcaga gcagctgtcg agtcgacgta cgaacgcgca
     4861 ccagtcttgg ggggggggg
//
LOCUS       DASTKU010000415        20642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_299526, whole genome shotgun sequence.
ACCESSION   DASTKU010000415 DASTKU010000000
VERSION     DASTKU010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 20642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20642
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299526"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(487..1884)
                     /gene="sthA"
                     /locus_tag="VFQ61_14210"
     CDS             complement(487..1884)
                     /gene="sthA"
                     /locus_tag="VFQ61_14210"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HET9955663.1"
                     /translation="MPFDQEHDLVVIGSGPAGEGATMRAAKAGARVVIIERASAVGGG
                     CTHWATIPSKALRHAVTQVLSFRRSRLFARYANQVEATFPSMLRAAEGVIERQVTMRS
                     EFYSRNHVAVVHGHARFEGEHTVVVERDGTRRRIHAKKFILAVGSRPYRPADVDFSHP
                     CVHDSDTILKLDHTPDTITVYGAGVVGCEYASIFRNLGVKVDLLNSRDKLLSFLDDEI
                     SDALSYHLREQGIVIRHNEEYASVEPQADGVVTRLKSGKRVKSDILLWAQGRTGNTQD
                     LGLDSVGLAPDSRGQLKVDEYYRTSCEHIYAVGDVVGYPSLASASYDQGRFAAGHAIE
                     GKIDEKLVQDIPTGIYTSPEISSLGRTERELTAAGVPYEVGRSLFRSLARAQITGNTV
                     GMLKLLFHRETLQILGIHCFGDQAAEIIHIGQAIMAQPGSANTLAYFANTTFNYPTMA
                     EAYRVAALNGLNRLF"
     gene            complement(1971..2558)
                     /locus_tag="VFQ61_14215"
     CDS             complement(1971..2558)
                     /locus_tag="VFQ61_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955664.1"
                     /translation="MSRRFLAATCWLLACGPNAAPARDPASVGDSANQTGPAAPTQLS
                     LSESAATPAQGGSANANVRPIDLAAMAIAGSGPSLPAVSLRTLGLHIGGGPNDTAGKA
                     PFVTAIESRFEDFLSCYRWVDEPGKSGTFGVDLHIERRGGKPRVEEPRAGLSGDKFQA
                     CVVRAFENVQFPSPGKPTVISYSLRFSVDNKPGNP"
     gene            3008..4009
                     /locus_tag="VFQ61_14220"
     CDS             3008..4009
                     /locus_tag="VFQ61_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955665.1"
                     /translation="MMVDKKDALSVVKRSVQLQRVQAQGAPTQGARKVSSFAALERSQ
                     DEFAPYAHGPALVGPHAYWVSQKRLVRRRLDGTGELEVLTQDAQNATRVAGWMNGSEA
                     WVAYITQPDKEGARHAKLWTEGHGTHDLTPEGAGSSSVGLTRARGGVLAVTLDGRSGM
                     TPLHTRLLTSEPAGVRLGPDVVSWVGGSSQGTTELFTTTTSNDEAWAFVPIEQDVTHF
                     GLAQIQLGTAPNPDASAEFIVFANGMNASVVASVQACGQTWVAFARPVKAVEGTQGSS
                     PQELVLARLDATGLNQPTLLAKARVFADVSLASSTGGAMLAYTADHRTWAASLWCRS"
     gene            4133..5092
                     /locus_tag="VFQ61_14225"
     CDS             4133..5092
                     /locus_tag="VFQ61_14225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9955666.1"
                     /translation="MPKKTQASNSARARKSTSRRVPAASGARSSTSERKRKSPAPRVA
                     KAAGQGTRRKAKKARLTAATADRHVLYQKSVQDAAVEIDFIDRTFKKLRKRPPLSLRE
                     DFCGTGLLTTAWVASDAKRTGIGVDIDPEVLAWGKKHNLEPLGEEGARVSLRKQDVRT
                     PPTEKVDVVVAFNFSYWIFMTREEMRKYFALVRKSLKKDGVYFLDAYGGWESHQPMQE
                     PRSIRGGFKYVWDQATFDPITHRAENHIHFRFPDGTEMEKAFSYTWRFWSLPEIQELL
                     QEAGFSKVQVYWDTSEDENEENYKPVKHADNHPGWLAYIVAQP"
     gene            5089..6039
                     /gene="gluQRS"
                     /locus_tag="VFQ61_14230"
     CDS             5089..6039
                     /gene="gluQRS"
                     /locus_tag="VFQ61_14230"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HET9955667.1"
                     /translation="MTPTVGRLAPSPTGLLHLGHARSFLLAWWSARAQGGRVVLRLED
                     LDAERAHPEFIAGTLRDLEWLGLDWDGEALLQSSELERLNANIQSLLERGLAYPCTCT
                     RSDLQQSQSAPQQGTLELRYPGTCRGRYTSMAEAEARSGRRPGIRLVVPSGTQHFDDR
                     LRGPQTFDVQAETGDFLIARRSGMPAYQLAVVVDDAHQGVTEVVRGEDLLSSTARQIC
                     VRQALRLPEPRVAHVALVVDDTGRRLAKRAASLGLSELRARGIDAESVVAWAARTAGF
                     HELGARASAREVLEIFELDRWAKQEPRQVPAPTPDSLIQN"
     gene            6119..7546
                     /locus_tag="VFQ61_14235"
     CDS             6119..7546
                     /locus_tag="VFQ61_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HET9955668.1"
                     /translation="MTDAPAASPALPVTQFERRFKHPPGLVILFFAEMWERFSYYGMR
                     GLLKLYMANYLFVTARESLQGCRQRTPACTLVPGDPSQVLFWDSLRGLLPDNPQETAS
                     LLYGTYTALVYATPFFGGILADRYLGQRKAVVLGGVLMALGHFVMALENSFFIALGLL
                     ILGNGAFKPNVSTQVGALYPPNDPRRDGAYTIFYMGINLGAFICNLVCGTLAVLYGWH
                     YGFAAAGVGMLLGLSVYLAGQKYLAPDNLAQHPEQKGGHTEKLNPDERRRVLALVVLC
                     ALNIVFWAVYEQQGNTMQSWADEHTHWPTVFGFTIPSTWFQSINPLTIILLAPLLDRF
                     WAYQNRHGKEPSSVTKMALGCIVLGGSFIVMIVGASVVGDGKGSLFWPAFCTLLLTVG
                     ELYLSPIGLSLVTKVSPARIVSMMMGMWFLSSFLGNFLSGAIGVLYTRLPKEAFFGLL
                     TLLGVSAGLAMLAFNRPLRNAMGKH"
     gene            complement(7602..8750)
                     /locus_tag="VFQ61_14240"
     CDS             complement(7602..8750)
                     /locus_tag="VFQ61_14240"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9955669.1"
                     /translation="MNPALRVARLFGIDVRVHFSFLIVLLLGALEFGRLHGPRGAMFG
                     ALLVLALFACVTLHELGHSLVARRFGGHVQDIILLPIGGVARMLREPTRPIHELWVAV
                     AGPAVNVLIAIGLGVGLGLNVEQLQRGPEGLGLVERLDGRAFLAWLFTFNVSLALFNM
                     IPALPMDGGRVLRAVLAMIVDRARATVWSANIAQVLALGFVFLAFQLQNPILLVIFGF
                     VFLGAGQEKRDARASLLLSDMTAGEVCHPGLEVLSYTDTLGDVVDHALRGNQSVFPVI
                     AGSDVRGVVLRDEAVEAAMRLGLKAHVSAVLRSQLHVCTPDTPLTAVRELLATVALPV
                     VVADGSRLVGVLSREDWMRITQLAARLSGAGIRRPRSVPEPSAPASSP"
     gene            complement(9287..10006)
                     /locus_tag="VFQ61_14245"
     CDS             complement(9287..10006)
                     /locus_tag="VFQ61_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9955670.1"
                     /translation="MQLRVHAAGAFEPTPHWLVSDGDLIVGPIATELLVRGVRSGRIP
                     PECSVCSTAGGDFRRIDQVREIRAALSPAVSEAGPERRTGARSAVQWLSDARDANEVL
                     LLALHGACELAFAQVGALYRVRFPLDLPVLSASYGDPCLDLGEVVPRRDAAWQIAQEG
                     EPVALRPRLSAAARAMESRLCIDREPMGLAIVPIRTATELFGVVELARFDHPFRAADV
                     RALVPLMSAGVARLEELSPAA"
     gene            complement(10093..11433)
                     /gene="rlmD"
                     /locus_tag="VFQ61_14250"
     CDS             complement(10093..11433)
                     /gene="rlmD"
                     /locus_tag="VFQ61_14250"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET9955671.1"
                     /translation="MNPRHPEAIWHIDKLVPGGEGLARLADGSVGFAAGVLPGEHVEV
                     VGSERKKGFVRATRLRVVTPAAERCEPPCVYARDCGGCDFMHIRPERQLDFKAGLIRE
                     ALSRTGGFAEFPDITLHPSPKAQGYRVRLRVHLQPGGRVGFFSERSHHLVPIEACLMA
                     RPELNAALAQLRRIAAEHPKLAEAFGEAELRVAPAREGCVLRLVPRANAAGGPRKQSS
                     FASALAALEQVFAVHIDGQRDDSEQTYSLTFAAESAAGEPQLEAIRLQVPASAFVQVN
                     WEVNCALVEHIVGGALERRAKRFLDLYSGVGNFTLPLARAGLGGEAVEANRGAAEAAR
                     RGLAASNLPVEVRAEPAEQFVQRLATRVAQTGAREPFDLVVLDPPRAGARDLIEALAR
                     LAPRFIAYCACDPVTLARDLKSLCAGGYALDSIHAFDMFPGTHHVETLVWLRRM"
     gene            complement(11454..11828)
                     /locus_tag="VFQ61_14255"
     CDS             complement(11454..11828)
                     /locus_tag="VFQ61_14255"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9955672.1"
                     /translation="MKLHHLALGSPNVERAASFYVKVIGLRELQRHNDSSGELRSIWL
                     TLDDAILMIERTQEPLRPVSGVGSGLFLIAFRVQPDGRREIEQRLIEYGQIIEAASEW
                     TFYTRDPDGNRLAFSYYPLPTS"
     gene            12349..12651
                     /locus_tag="VFQ61_14260"
     CDS             12349..12651
                     /locus_tag="VFQ61_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955673.1"
                     /translation="MKAAVEPVRENATKDEPTNVRAREESPPSSRMVPAPAQRELRMN
                     REHRIHDKVAEARLLLDKLPEGDSRRRLLLAAQARQDEVLLDAILSTLTRDCGPSR"
     gene            complement(12697..13500)
                     /locus_tag="VFQ61_14265"
     CDS             complement(12697..13500)
                     /locus_tag="VFQ61_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955674.1"
                     /translation="MKTSSSSGANEAPRVTQDPFGLGQQKLAAQVGDFVLAPSRASLE
                     RAMNEPHGVHTFAYLGAHVEQVGETHARLRWSTAQRGNVPNELVIPITREAVAELGGV
                     LLTATPGFGLERVLVEAGGTPNAPRVISLDRACLTLDAAQPEPVTLEKGTFQPLRQAG
                     ELGATLSCRSEGHVMPWILTAKTPERWLAWGFSGQVRTFAASECKTLPILPEVRVGEN
                     VFVPVAGEYVNATVRRVEANTGRVFVQHDFAAEAREHCVPLTQVARELP"
     gene            13742..15145
                     /locus_tag="VFQ61_14270"
     CDS             13742..15145
                     /locus_tag="VFQ61_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955675.1"
                     /translation="MRTTSAKKLRASVIVPAAWAAICLLPASASGANAKSPKPGATSA
                     LAEARAASPNGATPARNSNFETRKLPNGATLRVSAGTQLTLGRPIKVQTGPGRWNQAQ
                     SVQLLEGRVEVDVPSQCQPSCPVYVQAPRRVGAVAKAGRSVVIASAERVTLVALEGEM
                     LAASGNDWRHLSEGTVREFAPGAGPVDHAVMKAPETTLSAPIALSLSGGKAGLTAKLS
                     AVPGAARYELDLYQLDGSKRTFLERRESDTPTTSFENLAPGSYGVVARAVERTGVEGQ
                     ESAQKTFRVVGAELPVGATLVEGGILLPVRERVRLSGTEGIEVSYGRTAEFIRAPSSI
                     GVIRNSPTLVRLRAEGSAEELAMTLSPRVMRADIELKPARARWPEDDVRVAVKLLDGM
                     NRPLPEGSPLKPTVFVNAKRVDIGWTRANGTLVATVPKSNERGPWVVRVEVIDDSGAV
                     VGRDFLEVAASAKRAAR"
     gene            15197..15943
                     /locus_tag="VFQ61_14275"
     CDS             15197..15943
                     /locus_tag="VFQ61_14275"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET9955676.1"
                     /translation="MSRSAPALRDDSLLPSLHAVHRTRIRPFQAVLFDLDGVLLDTET
                     LYTVGIQEVVGSFGKTYDWALKRDMMGKSELVGAHLLIERLGLPLHAEEYLARVNPVF
                     ERLFHSVEPMPGAVALVEYLLERQVPLAIATSSRSHLFALKSRPHGWLHSIRAVVCGD
                     DAQVKALKPAPDIFLAAAARLQVPPAACLVVEDSPAGVSSALAAGMQVVALPDPALGH
                     DAVAHATTIVSSHDELRNLLSALLEAGELP"
     gene            complement(15886..17631)
                     /locus_tag="VFQ61_14280"
     CDS             complement(15886..17631)
                     /locus_tag="VFQ61_14280"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HET9955677.1"
                     /translation="MDAGTQSFQRRWLVPIVLLSTAAFVVFWGAYAFDYFYGGKVYAT
                     PDRPAAGGPVDHYLRYDPGSITDAVSALAGMIAAVFGIVITVVSIIVQLSAGRYAGVA
                     RMFMRDRVNIGVMAFYVIACVCGIWMSVSVQHDFVPRATLTGMLLASSAGLLVMAPYF
                     GYVFWFVEPTNIIRRIEREAAATARTGAVGRRARGTWLAPWTLVSPAARAVRIDDAQA
                     AVVAAMEELTDITSNSISGKDKIIASGAVDALRDLALAYIPAKSQAIEGWFVLCQALR
                     KNPDFVAMDPESLADLEQRKTWVEWKVMREYLGIYNDALPSMRDINYLIAINTRYLGE
                     AAARARDPELVRLVFRFMNSYLRATLNARDVRTAYNVLNQYRLLIEAMLAAELHEFVS
                     EGVRHMTYYGHVSFDMKLSFVTETVAYDVSALCQSAHERGSAAELEILDQFLELDRPL
                     RPQGQQGALLGVRKAQVKLGAYYVAHAELEKARRIARDMSGEPRERLLAIRQALEAVT
                     SKDFWEIIDRGRNFEYMPPHQRAALNEFFSWFTEKPEREAQMKEGLLKEGQSREAQGS
                     SPASSSADSRLRSSS"
     gene            18081..19247
                     /locus_tag="VFQ61_14285"
     CDS             18081..19247
                     /locus_tag="VFQ61_14285"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9955678.1"
                     /translation="MLDHRLSREWSERPGTARRHAACDRGARRYRSTSHALLLMLSLN
                     ATAALGTVGSRAFAQTDEQRAAARSLATDGAAAFNEGRYKESVDYFTRAESLVHAPPH
                     LLFSARALEKLGLLVKAREHYLKVVKETLPPTAPQAFRDAQTAAAQELAKVEPRIGSL
                     TLQITGAEQAKDLQVQVDGVALPAVLIGVARPTDPGEHIISLSAPGYKAYSETVKLSD
                     GEKKAVTLALQPDANAALPAPPPVEGQVGPSRPAATSTVDLDTSATAPPSSGQALKIG
                     GYVGLGVGVVGLGLGTVFLLNSRSKRADADSAFAQCNQTGDCRENDTAAQRTADLDDQ
                     ARSAQTISTVGFVVGALGIAAGTTMLVLGNQRTQSGLLVQPYVALGRAGVSGSF"
     gene            19318..>20642
                     /locus_tag="VFQ61_14290"
     CDS             19318..>20642
                     /locus_tag="VFQ61_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955679.1"
                     /translation="MLAPLWKTSRWLTLAVLAGTAVTACASGADSSPDPDEAGVDLAD
                     SSQAITLCTTDGMACSVTTRETGVCVNGSCCTGCVNRVLIGTKGACAVGDSLTMCGSG
                     GDACRNCDDNNPCTADACTAKGCTHTIVEGSCSGGVCVQGQCCTGCIDGDGNCQKGLS
                     PDGCGRGGGKCTTCFDRQPCTFDLCLEGSCQYPQKDDNTSCESDKDVCNGESICRGGK
                     CVAGTPLNCDDNNPCTDDKCDPAAGCQKSPIEGSCNDGDPCTISDTCVEGSCKGGATA
                     TCNDNEVCTQDLCDSTGSGCKNVPIDQGKDCDDGNSCSTASICDNGKCVTTAGTACDD
                     NNPCTRNRCTGGAGCYYTDLEPVNTPCIPANNKCVLNATCNETGACTGGEEKSCDDNN
                     PCTEEHCDPQSGECVSSKLNTGECNDGSACTVDDSCQDGVCQGTAKECPT"
BASE COUNT         3906 a         6472 c         6674 g         3590 t
ORIGIN      
        1 atcgagttat gtttcgccgc cccgaatcta cacctcgcta tcacctcagt tagccacttc
       61 gctagttgct tggcggctgt tcactcggct gggtcactcg gctacactct ccatcgtcgc
      121 gacttcggag ttcgtggcga gcagtcgacg ggtaactcga ggtctgattt gcgccgtgca
      181 atcacgggcg cgccgcccgt caaagcccac gccagcgcag ccgctcacgc gcccagttct
      241 cccatcaccg cgcctgcgcc cccattcgac gcgccgcgaa gttgcccaca ccacgctcgt
      301 agactcgcct gttggcgacc tgagaaaggg agggccggcg cggctgcggg ctgcaactgc
      361 gcgggctccc gcgggcgcac ggcgcagccc cacgcggcgt cgcgctcttt gcaaagcggc
      421 ccgccccata ggaacgcaaa gcaggccctt gcttagaacc tcggctgggg aggcgcgaga
      481 ttcaggctag aacagtcgat tgagcccgtt gagcgccgcc acgcgatagg cttcggccat
      541 ggtcggatag ttgaaggtcg tattggcgaa gtaagcgagc gtgttcgccg agcccggttg
      601 ggccatgatg gcttggccga tgtggatgat ctcggcggct tgatcgccaa agcagtgaat
      661 gcccaggatc tgcagcgttt cccggtggaa gagcagcttg agcatgccca cggtgttgcc
      721 ggtgatttgc gcgcgcgcga gggagcgaaa caacgaacgg cccacctcgt agggaacgcc
      781 agcggcggtg agctcgcgct cggtgcggcc gaggctggag atctcgggcg aggtgtagat
      841 gccggtgggg atgtcttgga cgagcttctc gtcgattttg ccttcgatcg cgtggcccgc
      901 ggcgaagcgg ccctgatcgt agctggcgct ggcgaggctc ggataaccga cgacgtcccc
      961 cacggcatag atgtgctcgc aagacgtgcg atagtactcg tcgactttga gctggccgcg
     1021 gctgtccggc gccagtccga cgctgtccag ccccaggtct tgggtgtttc cggtgcgccc
     1081 ttgggcccag agcaggatgt cgctcttgac ccgcttgccg ctcttcaagc gggtcaccac
     1141 gccgtctgcc tgcggctcga cgctggcgta ctcttcgttg tggcgaatga cgatgccttg
     1201 ctcgcggagg tgataggaca aggcgtcact gatctcgtcg tccaaaaacg aaagcagttt
     1261 gtcgcggctg ttgagcagat cgaccttcac gccgaggttg cggaagatcg aggcatactc
     1321 gcagccgacc acgcccgcgc cgtagacggt gatggtgtcc ggggtgtggt ccagcttgag
     1381 gatggtgtcg ctgtcgtgga cgcaaggatg ggagaagtct acgtcagcgg ggcgatacgg
     1441 ccgggagccc accgcgagga tgaacttctt ggcatggatc cggcgacgcg ttccgtcgcg
     1501 ttcgaccacg accgtgtgct ccccctcgaa ccgcgcatgg ccgtggacga cggcgacgtg
     1561 gttgcgggag tagaactcac tgcgcatcgt gacttggcgc tcgatcaccc cttccgccgc
     1621 gcgcaacatc gaggggaacg tggcctcgac ctggttcgca taacgagcga acagccgact
     1681 acgccgaaac gagagcacct gcgtgaccgc gtggcgcaga gctttgctcg ggatcgtcgc
     1741 ccaatgcgtg caaccaccgc cgacggccga ggcccgctcg atgatgacga cgcgcgcgcc
     1801 cgccttggcc gcgcgcatgg tcgcgccctc accagcgggc ccactgccta tgacgactaa
     1861 atcgtgttcc tggtcgaacg gcaaagtacg ggggtcatag cgcgatccgc ggctcagact
     1921 ccagaaactg tttcaatcgc aggcggatcc gggtggaaag cggtcgtctc tcacggattg
     1981 ccgggtttgt tgtcgacgct gaagcgcaac gagtagctga tcacggtggg cttgcccgga
     2041 gacgggaact ggacgttctc gaacgcgcgc acgacgcagg cttggaactt gtctccgctc
     2101 aggcccgcgc gcggctcttc gacgcgtggt ttgccaccac gcctttcgat gtgaagatcc
     2161 acgccaaagg tgccgctctt gccgggttcg tccacccagc ggtagcagct caagaagtct
     2221 tcgaaccggc tctcgatcgc ggtgacgaag ggggccttgc cggcggtgtc gttggggcca
     2281 ccgccaatgt ggagccccaa agtacgcaag gaaaccgcgg gcaagcttgg tcctgagccc
     2341 gcgatggcca tggccgccaa gtcgatgggt cgcacgttcg catttgcgct gccgccttgc
     2401 gccggcgtgg ctgcggactc gctgagcgag agctgggtag gggcagcggg gccggtttgg
     2461 ttcgcggagt ctcccacgct ggcgggatcc cgcgcggggg ccgcgttcgg gccgcatgct
     2521 agaagccagc aagttgcagc caggaatctg cgcgacatgg cgagatgcta acgctgagtg
     2581 gctgacctag gatacccctc gcatgatggg ccggtacgaa atggttccga gcgaacacgc
     2641 agaaggccga actgcgaggt ctcgagtacg cgtgtcggtc ggccgctgtg ccgcgggttc
     2701 ggtcctggtg ctggcctccc taggcgtact gacgagctgt agagcgcgca ccgacccgga
     2761 caatgcaact cgtgctcgac cgtcgagtca gcctctggcc tcgacgccgc gctctgagcc
     2821 tagggcccaa cctacggccg gtccatcgag ctctggcagc gatactggca gcacgtcgaa
     2881 aggcgccgcg cggggcccgc gtcagctcga actgagcgga aacagcgccg gaagtggcgc
     2941 ttggacgatc gaaagcccga gcgcaattgc tccttccgga gccgcaagcg ctgacgcaaa
     3001 cggcgtcgtg atggtcgaca agaaggacgc gctgagcgtg gtgaagcgca gcgtgcaatt
     3061 gcaaagagtt caagcccaag gagcgccgac gcaaggagca cggaaagttt cgagctttgc
     3121 cgcactcgag cggagtcagg acgagtttgc gccgtatgcg cacggcccag ccctggttgg
     3181 cccgcatgcc tattgggtga gtcaaaagag gctcgtgcga cgtcgcctcg acgggaccgg
     3241 agagttggag gtactcacgc aagatgcgca aaacgcaacc cgcgtcgcgg gttggatgaa
     3301 cggcagcgaa gcctgggtgg cgtacatcac ccagcccgat aaggagggag ctcgccatgc
     3361 caagttgtgg acggagggcc atggcactca cgatttgacc ccggaaggag cgggctcgag
     3421 tagcgtgggg ctaacacgcg cgcggggagg cgtgctggca gtgactttgg atggccgcag
     3481 cggcatgacg ccgttgcata cgcgcctgct cacgagtgag cccgccggag tacggctcgg
     3541 ccccgacgtc gtgagctggg tgggcggctc ttcccaaggc accaccgagc tgttcacgac
     3601 cacgacgagc aatgacgaag cctgggcgtt cgtgccaatc gaacaggacg tcacccactt
     3661 cgggttggct caaatccagc ttggaacggc gccgaacccg gacgcgagcg cggagttcat
     3721 cgtgttcgcg aatggcatga acgcctcggt ggtcgcgagc gtgcaggcgt gcgggcagac
     3781 ctgggtcgcc ttcgcgcgac cggtgaaggc cgtcgaagga acgcaaggca gttcccctca
     3841 ggagctggtg ttggctcggc tagatgccac gggcttgaat cagccgacgt tgctggccaa
     3901 ggcccgcgtc ttcgcggacg tgtcgcttgc gagcagcacc ggcggggcga tgctcgccta
     3961 caccgccgac caccgtactt gggcggcttc gttgtggtgt cgcagctgag agctgatggt
     4021 tctcgaaagt gacgtccggg ctgaatcgag ccccttcaca cgagttcctg ctcctcggcc
     4081 gcgaaatctg ggttaaacag ggggcctgtt tcgcagccct cacgaaccac acatgccaaa
     4141 gaagacccag gcttcgaatt ctgctcgcgc ccgcaagtcg acctcgcgac gcgtacccgc
     4201 cgcaagcggc gctcgttcga gcacctccga acgtaaacgc aagagccccg cccctcgcgt
     4261 ggccaaagct gcaggccaag gcacccgccg taaagcaaag aaggcgcggc tgaccgcggc
     4321 cacggcggac cgtcacgtgc tttatcaaaa atccgtgcaa gatgcggcgg tcgagatcga
     4381 cttcatcgac cgtacgttca agaagctgcg caagcgtccg ccgctctcct tgcgcgagga
     4441 tttctgcgga actgggctgc tcacgactgc ctgggtcgcg agtgacgcga agcgcactgg
     4501 catcggcgtc gacatcgatc ccgaggtgct cgcgtggggc aagaagcaca acctcgagcc
     4561 gctcggtgaa gagggcgccc gcgtgagtct ccggaagcaa gacgtgcgca cgccgccgac
     4621 cgagaaggtc gacgtggtgg tcgcgttcaa cttcagttac tggatcttca tgacacgcga
     4681 ggagatgcgg aagtacttcg ccctcgtgcg gaaaagccta aagaaggacg gggtgtactt
     4741 cttggatgcc tacggcggtt gggagtccca ccagcccatg caggaaccgc gttcgatccg
     4801 cggcggcttc aagtacgttt gggatcaagc aaccttcgat ccgatcacgc accgcgcgga
     4861 aaatcacatc cacttccgct tccccgacgg gacggagatg gaaaaggcat tcagctacac
     4921 ttggcgattc tggtctttgc cggaaatcca agagctgctc caagaagccg gcttctcgaa
     4981 ggtgcaagtg tattgggaca ccagcgagga cgagaacgaa gagaactaca agcccgtcaa
     5041 gcacgccgat aatcacccgg ggtggttggc gtacatcgtc gcgcagccat gacaccgacg
     5101 gtggggcggc tcgcgccgag ccccacgggg ctcttgcacc tgggccacgc acgcagcttc
     5161 ttgctcgcgt ggtggtcggc ccgggcgcaa ggagggcggg tggtactgcg gctcgaagac
     5221 ctggatgcgg agcgggcgca tccggagttc atcgcgggca cgttgcgaga tctggagtgg
     5281 ttgggcttgg attgggatgg ggaagcgttg ctgcagtcga gcgagctcga gcgcttgaat
     5341 gcgaatatcc agtcgctgct cgaacgaggg ctggcatatc catgcacctg cactcgctcg
     5401 gatctgcagc aaagccagag cgctccgcaa caaggtaccc tggagctgcg ttatcccgga
     5461 acctgtcgcg gccgctacac gagcatggcg gaagccgagg ctcgcagcgg tcgccggccg
     5521 gggatccgtc tggtcgtgcc gtcggggacg caacacttcg acgatcggct gcgcgggccc
     5581 cagaccttcg atgttcaggc cgaaacgggg gactttttga tcgcgcgtcg gagcgggatg
     5641 ccggcttatc aactggcggt ggtggtagac gacgcgcatc agggcgtgac cgaggtggtg
     5701 cgcggggaag atctgttgag cagcaccgcc cgacagatct gcgttcggca agctttgagg
     5761 ttgcccgaac ctcgggtggc gcatgtggcc ttggtggtag atgacaccgg ccgccgtttg
     5821 gcgaagaggg ctgcttcttt ggggctgagt gagctgcgag cacgcgggat tgatgccgag
     5881 tccgtcgtgg cctgggccgc gcggactgcg ggtttccacg agctgggtgc gcgagcgagc
     5941 gcacgcgagg tactcgaaat cttcgagctc gaccgctggg cgaaacaaga gccgcgccag
     6001 gtgcccgcac ccacaccaga tagcctgatt cagaactgag accgcaagat ttcggcgcta
     6061 gcacctcgcg cgcagcgctc tggcgtgacg ggtgttgggc agtgtaacgt cgcaagtcat
     6121 gaccgacgcg cccgcagcta gcccggcact cccggtgacc cagttcgaac gtcgcttcaa
     6181 gcatcctccg ggattggtca tcctgttctt cgcggagatg tgggaacgct tcagctatta
     6241 cggcatgcgg gggctgctca agctgtacat ggcgaactac ctgttcgtga ccgcgcgcga
     6301 gtcgctgcaa ggttgccggc aaaggacgcc cgcttgcaca ctggtgccgg gagatccgag
     6361 ccaggttttg ttctgggact cgttacgcgg tttgctgccg gacaatccgc aggagaccgc
     6421 gtctttgctg tacggcacat acacggcgct ggtctacgcc acgccgttct ttggcgggat
     6481 tttggcggac cgatacttgg gccaacgcaa ggcggtggta ctgggcggcg tgctgatggc
     6541 gctcgggcac ttcgtgatgg cgctcgaaaa ctcgttcttc atcgcgctcg gccttttgat
     6601 cttgggcaac ggcgcgttca agcccaatgt ctcgacccag gtgggagcgc tgtacccgcc
     6661 gaacgatcca cgccgcgacg gggcgtacac gatcttctat atgggcatca atctcggcgc
     6721 gttcatttgc aacctagtct gcggcacgct cgcggtgctg tacggctggc actacgggtt
     6781 cgcagccgcc ggtgtgggca tgctgctggg attgagtgtg tacctcgcgg ggcagaagta
     6841 cctggcgccg gacaacctcg cgcagcaccc cgagcagaag gggggccaca cggagaaatt
     6901 gaaccccgac gaacggcggc gagtgctcgc gctggtggtg ctgtgtgcac tcaatatcgt
     6961 gttttgggcg gtgtacgagc aacaggggaa caccatgcaa tcttgggccg acgagcacac
     7021 gcattggccc accgtgttcg gattcaccat tccctcgacc tggtttcagt cgatcaaccc
     7081 actcacgatc atcctgctcg cgcccttgct cgataggttc tgggcctacc agaaccggca
     7141 cggcaaggag ccgtcgagtg tgaccaagat ggcgctcggc tgcatcgtgc tgggcggttc
     7201 tttcatcgtg atgatcgtgg gcgccagcgt ggtgggagac ggtaagggta gcctgttctg
     7261 gccggcgttc tgcacgttgt tactcacggt tggtgagctc tacttgtcgc cgattgggct
     7321 ctcgctcgtg acgaaggtct cgcctgcccg catcgtgtcc atgatgatgg ggatgtggtt
     7381 tctctcgagc ttcctcggta atttcctgag cggggcaatc ggtgtgctgt acacgcggtt
     7441 gcccaaggaa gccttcttcg gactactaac cttgcttggg gtcagcgccg ggctcgccat
     7501 gttggctttc aatcgcccgc tccgaaacgc gatgggtaaa cactgaagct cacgcccggc
     7561 gccatgggct ccagacgctc agaccgatcc caagagtgct cttaggggct gctcgcggga
     7621 gcggatggct ccggcaccga ccgtgggcgg cgaatacctg caccggagag gcgcgccgcg
     7681 agctgcgtga tgcgcatcca gtcctcgcgg gacagtacac cgaccaaacg gctgccgtcg
     7741 gccacgacca ccggtagcgc caccgtggcg aggagctctc ggacggcggt gagcggcgta
     7801 tcgggcgtgc aaacatgcag ttggctacgt aagacagccg atacgtgagc cttcaacccc
     7861 aagcgcatcg ccgcctcgac ggcctcgtcc cgcagcacca cgccgcgtac atcgctgccc
     7921 gcaatcaccg ggaacacgct ttgattgccg cgtagcgcgt gatcgaccac gtccccgagc
     7981 gtgtccgtat aagacagcac ctcgagcccg ggatggcata cctcgcccgc cgtcatatcg
     8041 gagagcaaca ggctcgcgcg tgcgtcccgc ttctcctggc ccgcacccaa aaacacgaac
     8101 ccaaagatca cgagcaagat tgggttttgg agctgaaaag ccaagaaaac gaaaccgagc
     8161 gccagaactt gcgcaatgtt ggcggaccaa acggtcgcgc gtgctcggtc gacgatcatg
     8221 gcgagcaccg cgcgcagcac tcggcccccg tccatcggca gggcgggaat catattgaag
     8281 agcgccagcg acacgttgaa ggtgaagagc cacgctagga aagcgcggcc gtcgaggcgc
     8341 tccaccaagc caagcccctc cggcccgcgt tggagctgct ccacgttcaa ccccaatccc
     8401 acaccgagcc caatcgcgat caacacgttc accgcgggcc ccgcgaccgc cacccacagc
     8461 tcgtgaatgg gtcgcgtcgg ctctcgcaac atgcgcgcca cccccccgat ggggagcagg
     8521 atgatgtctt ggacatgacc gccgaaccga cgcgcgacca gcgagtggcc gagttcatgc
     8581 aaagtgacgc aggcgaacaa cgccaggacc aggagagccc cgaacatcgc cccacgtgga
     8641 ccgtgcaggc ggccgaactc cagagcgccc agcaggagca ctatcaaaaa tgaaaagtgc
     8701 acccgcacgt cgatcccaaa caggcgcgcc acgcgcagcg cgggattcaa aggacgcccc
     8761 gcggcaggac gacggttcgg tagttctgaa gaaatccctg catggcgtgg ctcggacagg
     8821 ctttgggtgc agtgtgcggg caagaacggc ggttcagacc atccaaagcg cggaggagcc
     8881 gcgtctctgg ttgcctgatg tgcgtctgcc taggattgcg cgcaagtgca agcgctcacg
     8941 gccgctcgga tcgcgcttcc agcatcaatc ccgcgcccat cactcacatc ccaaatcagc
     9001 tcgttttttt tcgagagcga actgcgttgt ttcacaattg caatgcgcca ctacatgcga
     9061 gctcggtatg tcttcgaatt tagccgggtc tcgcacggca cggatactgc acttatgggt
     9121 gactgcaggc ggcgcccttg gtgccccccc ctccgcctcg gtcgccgcct cccttcttct
     9181 cagcggtctg gagcgttccc ctcgctccag gccgttgtct tttttgttcg atgaaattgg
     9241 gttggttgcg gatcggccgc cacctggccg agatccatcg gagcattcag gcggcgggcg
     9301 agagctcttc gagccgcgcc actcccgcag acatcaaggg caccagagct cgaacgtccg
     9361 ccgcacggaa cgggtggtcg aagcgagcca attcgacgac tccgaatagc tcggtagcgg
     9421 tccgaatcgg cacgatcgca aggcccatcg gttcgcggtc gatgcagagc cggctctcca
     9481 tggcgcgagc ggcggcgctg agtctaggtc gaagcgcgac gggctcgcct tcttgagcga
     9541 tttgccaggc tgcgtcccga cgcggcacca cctcacccaa gtctaggcaa ggatcgccgt
     9601 agctcgcaga gagaaccggc aagtccagcg ggaatcgcac tcgatagagc gccccgactt
     9661 gggcaaacgc gagttcgcag gccccatgca gcgcgagcag cagcacctcg ttggcatcac
     9721 gtgcatcgga gagccattgg accgcactgc gagctccagt ccgccgctcg ggtcccgcct
     9781 cactgacagc cggagataga gcggctcgaa tttcgcgcac ttggtcgatg cgccggaagt
     9841 caccgcctgc cgtcgagcag accgagcact cgggagggat gcgaccgctc cgaacccctc
     9901 gcacgagtaa ctcggttgca atgggtccga cgatcagatc tccatccgag accagccaat
     9961 ggggagtggg ctcgaaagca cccgctgcgt gaacacgaag ttgcatcgaa acctcctggg
    10021 caacccgccg gaggttggag taggctccca agccgggcca actttgtcga agcaatcttg
    10081 gaagcgcggc ccctacatcc tgcgcaacca aaccaaggtc tcgacgtggt gagttccggg
    10141 aaacatgtcg aaggcgtgga tcgagtcgag ggcatagcct ccggcgcaaa gggacttgag
    10201 gtcacgcgcg agcgtcactg ggtcacacgc gcagtaagca atgaaacgag gagcgagccg
    10261 agccaaggct tcgattaagt cccgggctcc agcgcgcggt ggatcgagca ccactaagtc
    10321 gaagggctcc cgcgcgccgg tttgtgcgac tcgagttgcg aggcgttgaa cgaactgctc
    10381 ggcgggctcg gctcgcactt cgaccggcag attcgaggca gccaggccgc gccgcgcggc
    10441 ctccgcagcg ccccggttgg cttcgacggc ctcgccgccc agccccgctc gggcgagcgg
    10501 gagcgtgaag ttcccgacgc cggagtacag atccaagaag cgtttcgccc ggcgttcgag
    10561 tgccccaccc acgatgtgct cgaccagtgc gcagttcact tcccaattga cctgtacgaa
    10621 ggcgctagcg ggcacctgca ggcggatggc ttcgagttgc ggctcgcctg cggcggattc
    10681 agctgcgaag gtcagcgagt aggtttgttc cgagtcgtca cgctgcccgt cgatgtgcac
    10741 ggcaaacacc tgctcgagtg ccgccaaggc tgacgcgaac gagctctgct tgcgcgggcc
    10801 cccggcggcg ttcgctcgcg gcacgagacg aagcacacac ccttctcgag caggagccac
    10861 gcgcaactca gcctccccga acgcttcggc gagcttcgga tgttcggccg caatgcggcg
    10921 aagctgcgcg agcgcagcgt tcagctcggg tctggccatc agacaagctt ctatcggcac
    10981 taagtggtgg ctacgctccg aaaagaaccc cacacggcca ccgggctgca agtgcacgcg
    11041 gagccggact cggtagcctt gcgccttcgg tgaaggatgc agcgtgatgt ccgggaattc
    11101 cgcaaaaccg cccgttctcg agagcgcctc gcgaatgagg ccggctttga aatccagctg
    11161 tcgctcgggg cgaatgtgca tgaagtcgca cccgccgcag tcacgcgcat aaacacacgg
    11221 tggctcacag cgttcggccg caggggtgac gacgcgcaac cgggtggcgc gcacgaagcc
    11281 cttcttgcgc tcggagccga cgacctccac gtgttcgccg ggtagcacgc cggcggcaaa
    11341 tccgaccgat ccgtccgcga ggcgggccaa accctctcct cctggaacga gcttatcgat
    11401 gtgccagatg gcctctggat gcctggggtt cacgttcgct tcgtggttcg atgtcaggac
    11461 gtcgggaggg ggtagtagct gaaggcgagg cggttgccat ccggatcgcg cgtgtagaac
    11521 gtccactcac tcgcagcctc gatgatttgg ccgtattcga tcagccgctg ctcgatctcc
    11581 cgcctgccgt cgggttgaac tcgaaatgcg atcaggaaca agccgctgcc cacccctgac
    11641 actgggcgca gaggttcttg cgttcgctcg atcatcagaa ttgcatcatc gagggtgagc
    11701 cagatcgaac gcagctcacc actcgaatcg ttgtggcgtt gaagctcgcg caggcctata
    11761 accttcacgt agaagctggc agcgcgttcc acattgggcg aaccgagggc gagatgatga
    11821 agcttcatgg tggcggtgat gcagcctttc atcgggacaa ctggcgcgaa atcggtgatt
    11881 cgtggcgtat gggcttcagg tactccgctg tcttactagc tgatgttcta cccgtgggtg
    11941 ctcggaatct tcacgcgctc gagagcaatt ggcagagcga gggtctaagc gaccggcaca
    12001 ttggaccgaa gccacagaca cgtgatcgga taggacagac acgtggtccg atgcgacaaa
    12061 cagatgtacg aaggggtgat gtgcgaactg tcggtgtggt tcgcgccgac gcgtgcattc
    12121 ccaacgctca cctacggacc accgccaata ccgacactac ggaaatgctc atgcgcaggg
    12181 gacacccctt ggttatcgat tgagagcact ccatgttcga aggtgccggc acccaatact
    12241 cacacgtgag ccgagtctga tagtcagcgg aatgcttatg acggaccagg cgggaatgaa
    12301 tgacccgccg ttacggttca tccaacggga ttgagcgagg agaaactcat gaaagccgcg
    12361 gtcgaaccgg ttcgtgaaaa tgccacgaaa gacgaaccga cgaacgtgcg ggcgcgggaa
    12421 gaaagtcccc cctcgtcacg catggtgccc gctccggcgc aacgcgagct tcgcatgaat
    12481 cgcgagcacc gcattcacga caaggtggcc gaggcgcgct tgctgctcga taagttgcca
    12541 gagggggatt cgcgtcgccg ccttttgctg gccgcgcaag cgcgtcagga cgaggtgctg
    12601 ttggacgcga tcctgtccac gttgacgcgt gattgcggcc cgagccgcta gcgctcacgc
    12661 ccgtctcggc agactcgagg gctccccaag tacctactac ggaagttcgc gcgcaacctg
    12721 ggtgagcgga acacagtgtt ccctcgcctc cgcggcaaag tcgtgttgga cgaacacgcg
    12781 gcccgtgttg gcctcgactc ggcgcaccgt cgcgttgacg tattcacctg ctaccgggac
    12841 gaagacgttc tctccgacgc gcacctccgg caggatcggt agagtcttgc attcagaggc
    12901 cgcgaaggtg cgcacttgcc cggaaaaccc ccacgcgagc caccgctccg gggtcttcgc
    12961 cgtcagaatc caaggcatca cgtgaccttc ggagcggcag ctgagggtgg ctcccagctc
    13021 ccccgcctgt cttaggggct gaaacgtgcc cttctccaac gtaacaggct caggttgggc
    13081 ggcatcaagg gtgagacaag cgcgatcgag cgagatgacg cgcggcgcgt tgggggttcc
    13141 gccggcctct accaacactc gctccaaacc gaatcccggt gttgcggtga gcagcacgcc
    13201 tcctagttcc gccactgctt cgcgcgtgat cgggatgacg agctcgttcg gcacgttccc
    13261 gcgttgcgcg gtcgaccaac ggagccgagc gtgcgtctcc cccacctgct ccacatgcgc
    13321 gccgagatac gcgaaagtgt gcacgccatg cggctcgttc atggctcgct cgagggacgc
    13381 ccgtgaaggc gccaacacga aatctccgac ctgcgccgca agcttttgct ggccgaggcc
    13441 gaaaggatct tgtgtcaccc gtggagcctc gttggctccg ctgctggagc tggttttcac
    13501 gatcggatcg agcgcgctcg cctcgtctga cgcggtgcga gcgcagccac aacacgcgcc
    13561 gaggcaaaat gcccgtaaaa agagcgctga ccaaggcctc cgactcggct gaagtccgag
    13621 cctaatcgtc cgtacaagtt ccatcaggag agctgcgttg atgagtcgag gtctagcatc
    13681 gctgcgggtt gtctctacaa gcccttcgtc tatgcctgcg cgtgagacag gtcgccagga
    13741 ggtgcgtacg acgtccgcta agaagctgcg cgcgtcggta atcgtccccg cggcctgggc
    13801 tgccatctgc ctgctgcccg cgagcgcttc cggcgccaat gccaagagcc ccaaaccagg
    13861 cgcgaccagc gcgttggcag aagcgagagc cgcgagcccg aatggggcca ctcctgcacg
    13921 caactcgaat ttcgagacga ggaagctacc caacggcgca acgttgcgag tgtccgctgg
    13981 aacccagctc acactcggcc gaccgatcaa ggttcagacc ggtccgggac gctggaacca
    14041 agcccagtca gttcaacttc tcgaaggtcg cgtcgaagtc gacgtaccga gccaatgtca
    14101 gccgagctgc cccgtttacg tgcaagcccc gcgacgggtg ggagcggtgg ccaaagcggg
    14161 gcggagcgtg gtgatcgcat cagcggagcg ggtcacgctc gtcgcgctgg aaggcgagat
    14221 gctcgctgcc agcggcaatg attggcggca cctctccgaa ggcaccgtgc gtgagttcgc
    14281 tcccggagcc gggcccgtcg atcacgctgt catgaaggca cccgagacga cgctgtccgc
    14341 accgattgct ctgagtttgt ccggtggcaa agccgggctg accgcgaagt tgagtgccgt
    14401 gcccggtgca gcccgatacg agttggattt gtaccagctc gacggcagca agcggacgtt
    14461 cttggagcga cgcgagagcg atacgccgac gacgagtttc gagaatctcg cgcccggctc
    14521 ttacggagtg gtagcgcgcg cggtagagcg taccggcgtc gaaggccaag agagcgccca
    14581 gaagacattc cgggtcgtgg gcgccgagtt gccggtgggc gctacgctgg tggagggcgg
    14641 gatcttgttg ccggtgcgag agcgcgtgcg gctctcgggc accgaaggca tcgaggtgag
    14701 ctacggaaga actgccgagt tcattcgtgc gccgagcagc atcggcgtga ttcgcaattc
    14761 gcccacgctg gtgcgtttgc gcgccgaagg ctccgccgaa gagttggcga tgacgttgtc
    14821 gccgcgggtg atgcgagccg atatcgagtt gaagccggct cgcgccagat ggcccgaaga
    14881 cgacgtgcgc gtcgcggtga agttgttgga cggcatgaat cggcctttgc ccgagggctc
    14941 tccgctcaag ccgaccgtat tcgtcaacgc gaagcgcgtg gatattggct ggacccgtgc
    15001 caacggcacc ttggtggcca cggtgccgaa gagcaatgaa cgagggccgt gggtcgttcg
    15061 cgtcgaagtc atcgacgata gcggtgcggt ggttggccga gatttcttgg aagtggcggc
    15121 aagcgcaaag cgagcggccc gctgactcga atctggattg ccacccgacc cgagctcgcc
    15181 taagcggtgg gtgccgatgt cacgctccgc gcccgctctt cgcgacgaca gcctcctgcc
    15241 ctctttgcac gccgtgcacc gcacgcgcat ccgcccgttc caagccgtat tgttcgactt
    15301 ggatggggtg ctgctggata ccgaaacact gtacacggtg ggtatccaag aggtggtcgg
    15361 aagcttcggg aaaacttacg actgggctct caagcgcgac atgatgggca agagcgagct
    15421 ggtcggcgcg cacttgctca tcgagcgact ggggcttccg ctgcatgccg aggagtacct
    15481 cgctcgcgtg aacccggttt tcgaacggct attccactcc gtagaaccca tgccgggggc
    15541 tgtggcgctg gtggagtatt tgttggaacg gcaggttccg ctggccattg cgactagcag
    15601 tcgctcccac ctcttcgcgc tgaagagccg cccccatggc tggttgcaca gcatccgcgc
    15661 ggtagtgtgc ggggatgacg cgcaggtcaa ggcactcaag ccggcacccg acatcttcct
    15721 ggccgccgcc gcgcggcttc aggtgccgcc cgcggcgtgc ctggtggtgg aggattcacc
    15781 cgcgggagtt agctcagcgc tcgccgcggg catgcaggtg gtagcgttgc cggatcccgc
    15841 gctcggccat gatgcggtgg cgcacgcaac cacgatcgtg agctctcacg acgagctgcg
    15901 taacctactg tccgcgctgc tggaggctgg cgagctgccc tgagcttctc ggctctgtcc
    15961 ttccttcaac aatccttcct tcatttgagc ctctctttct ggtttttcgg tgaaccatga
    16021 gaagaactcg ttcaaggcgg cgcgttgatg aggcggcatg tattcgaagt tgcgtccacg
    16081 atcgatgatt tcccagaaat ccttggacgt taccgcctcc agggcctgac gaatcgcaag
    16141 taggcgttcg cggggctcgc cagacatgtc ccgcgcgatg cgccgcgcct tctcgagctc
    16201 cgcgtgagcc acgtagtacg cgccgagctt gacttgagcc ttgcgcacac cgagcagcgc
    16261 cccctgctgg ccttgcgggc gcagcgggcg atcgagctcc agaaactggt cgagaatctc
    16321 gagttcggcg gcggagccgc gctcgtgggc gctctggcaa agggccgata cgtcgtacgc
    16381 caccgtttcg gtgacgaagg agagcttcat atcgaagctg acgtgcccat agtacgtcat
    16441 gtgtcgcacg ccctcggaca cgaactcgtg gagctcggcc gcgagcattg cctcgatcag
    16501 cagccgatac tgattcaaga cgttgtacgc cgtacgcacg tcccgcgcgt tgagcgtggc
    16561 tcgcaagtag gagttcatga agcggaagac gagccgtacg agctccgggt cccgcgcgcg
    16621 cgccgccgcc tctcccaagt accgcgtgtt gatggcaatc aagtaattga tgtcacgcat
    16681 gctcggcagc gcgtcgttgt agatacccag gtattcgcgc atcaccttcc actccaccca
    16741 ggttttccgc tgctcgagat ccgcgagtga ctccggatcc atggccacga aatcagggtt
    16801 cttgcgcagc gcctggcaca gcacgaacca gccttcgatg gcttgcgact tggccgggat
    16861 gtaagccagc gcgagatctc gcaatgcatc caccgcgccg ctcgcgatga tcttgtcttt
    16921 gccggaaatc gagttgctgg tgatgtccgt gagctcttcc atggccgcaa cgacggcggc
    16981 ctgcgcgtcg tcgatacgca cggcgcgcgc cgcgggtgac acgagcgtcc acggcgcgag
    17041 ccaagtaccg cgcgctctgc gccccacggc gcccgttcgc gccgtcgccg ccgcttcacg
    17101 ctcgatgcgg cggatgatgt tggtgggctc gacgaaccaa aatacatagc cgaagtacgg
    17161 cgccatcacg agcaacccgg ccgagctcgc gagcaacatg ccagtcaacg tggcgcgcgg
    17221 cacgaaatcg tgctgcacgc tgacgctcat ccaaattccg cagacacacg cgatcacgta
    17281 gaaggccatc accccgatgt tcacgcgatc gcgcatgaac atgcgcgcga cgccagcgta
    17341 gcgaccagca gacaactgca cgatgatgct gacgaccgtg atcacgatgc cgaacacggc
    17401 tgcgatcatg cccgctaacg cgctcacggc gtccgtgatg ctgcccggat cgtacctcaa
    17461 gtaatgatcg acggggccgc ccgccgcggg ccgatccggc gtggcgtaga ccttccctcc
    17521 gtagaagtag tcgaacgcgt acgctcccca gaaaaccacg aacgctgccg tggatagcag
    17581 gacgatgggg accaaccagc gtcgttgaaa gctctgcgta cctgcgtcca tgtcagcggc
    17641 cactgtagac agacgctggc cgaagaacta ccccgccgca cctcacccca agaacctccc
    17701 ccaaaaagct gcccagaaaa ccgcccgaac tccggcccct gcccgaggcc cgctccgcgc
    17761 gtcaactggg cccgcgcgtg gcagaagcaa aacgaacgct tgtcgtggcc agcacgttgc
    17821 gaattcaacc tgactgatac ccagtgtccg cgtgtgtcgc agaccgactg cgcgccgccc
    17881 ggcgagctcg gcctgacgag cgagtgaacc gagcgagtga acatacaggc gcactcggac
    17941 atagcgcgag catcgaaccg cctgaacgcg cgtcagtcag tcgggaggag gatcgaagaa
    18001 atccccagtc ggagcccggc gcttctcgta aagaaatcct tcttgtcgtg gaggaggccg
    18061 gataagcttc tgtgatcctc atgcttgatc atcgcttgtc gagggaatgg tccgagcgtc
    18121 caggcacggc gcgcagacac gcagcgtgtg accgcggcgc gcgccgctat cgcagtacga
    18181 gccacgcgct gctgctcatg ctgtcgctca acgccacagc tgcgctcggg acggttgggt
    18241 ctcgggcctt tgctcaaacc gacgagcagc gcgcggccgc gcgctcgctc gcaaccgacg
    18301 gagccgcggc cttcaacgaa ggccgttaca aggagagcgt ggactacttc acgcgggcgg
    18361 aatcgctggt tcacgcaccg ccgcacttgt tgttcagcgc gcgagccctc gaaaaattgg
    18421 gcctgctcgt caaagcccgc gaacactatt tgaaggtggt caaggagacg ctgccgccaa
    18481 ccgcgccgca agctttccga gatgcccaga cggcggccgc gcaggaactc gccaaggtgg
    18541 agccacgcat cggaagcctc acgctgcaaa tcacgggggc agaacaggcc aaagatcttc
    18601 aggtgcaggt ggacggcgtg gcacttcccg cggtgctcat cggagtggct cgcccgaccg
    18661 atccgggtga acacatcatc tcgctgagtg cgccgggata caaagcctac tccgagacgg
    18721 tgaaactcag cgatggagag aagaaggcgg tcacgctcgc gctgcaaccg gacgccaacg
    18781 cggcgctgcc ggcgccgcca cccgtcgagg gtcaggttgg cccgagccgc cccgccgcta
    18841 cgagcacggt cgatttggat acgagcgcca cggctccacc gagctccggt caagcgctga
    18901 aaattggcgg ctatgtgggc ttgggagtgg gcgtggtcgg gcttgggctc ggcacggtgt
    18961 ttctgctgaa ttcgcgcagc aaacgcgcgg acgcggactc agccttcgca caatgcaatc
    19021 aaaccggtga ctgtcgagag aacgacaccg ccgcgcaacg taccgcggat ttggatgatc
    19081 aagcgcgtag cgcacagacc atctcgacgg tcgggttcgt ggtcggagcg ctcgggatcg
    19141 ccgcggggac caccatgctg gtgctcggca atcaacgcac acagagtggc ttactcgtgc
    19201 agccctacgt agctctcggt cgggctggcg tcagtggttc attctgacgc ctcgcatcgc
    19261 ttgcttcgcc gcgaatctca actgttcgtc acgagttcac ttcaggagtt cctcagaatg
    19321 cttgccccgc tttggaagac gtcccgctgg ttgactttgg ctgtgttggc cggaactgcc
    19381 gtcacggcct gcgccagcgg cgccgattcg agccccgatc ccgacgaagc gggggtcgac
    19441 ctcgccgact cgagccaagc catcacgctc tgcacgacgg acggcatggc ttgctcggta
    19501 acgacgcgcg agaccggtgt ctgcgtcaat ggctcgtgct gcacgggttg cgtgaaccgc
    19561 gtcttgatcg gcaccaaagg cgcgtgcgcc gtgggagatt ccttgacgat gtgcggctcg
    19621 ggtggagacg cctgccgcaa ttgcgatgac aacaacccct gcaccgcgga tgcttgcacg
    19681 gccaaaggct gtactcacac catcgtcgag ggctcctgct ccggcggcgt gtgtgttcag
    19741 gggcaatgct gtacgggctg catagacgga gacggcaatt gccaaaaggg gctctcgccg
    19801 gatggttgcg gccggggcgg tgggaaatgc accacgtgct tcgatcgcca gccctgcacc
    19861 ttcgatttgt gcctcgaggg ctcctgccag tatccccaaa aggacgacaa cacctcgtgt
    19921 gagtccgata aggacgtctg caacggcgag agcatttgcc gaggcggcaa gtgcgtggcc
    19981 ggcaccccgc tcaactgcga tgacaacaac ccgtgcaccg atgacaagtg cgaccccgcc
    20041 gcgggctgtc aaaagagtcc catcgaaggc tcgtgcaacg acggcgatcc gtgcacgatc
    20101 agcgacacct gcgtggaggg aagctgcaaa ggcggagcaa ccgcaacctg caacgacaac
    20161 gaggtctgta cccaagacct ttgtgactcg accgggagcg gctgcaagaa cgtgcccatc
    20221 gaccaaggca aggactgcga cgacgggaac agctgcagca cggccagcat ttgtgacaac
    20281 ggcaagtgcg tcacgaccgc gggcaccgcg tgtgatgaca acaacccgtg cacgcgcaac
    20341 cgctgtacag gcggcgccgg ctgttattac accgatctgg agccggtaaa taccccctgc
    20401 attccggcca ataacaagtg cgtcctgaac gcgacgtgca acgaaaccgg cgcgtgcacg
    20461 ggtggcgaag agaagagctg cgatgacaac aatccatgca ccgaggagca ctgcgatccg
    20521 caaagcggcg agtgcgtgag cagcaagctg aacacgggcg agtgcaatga tggatcggcc
    20581 tgcaccgtgg acgattcgtg ccaagacggc gtttgccaag gcaccgccaa ggaatgccca
    20641 ac
//
LOCUS       DASTKU010000416        10722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_524029, whole genome shotgun sequence.
ACCESSION   DASTKU010000416 DASTKU010000000
VERSION     DASTKU010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10722
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524029"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1092)
                     /locus_tag="VFQ61_14295"
     CDS             complement(130..1092)
                     /locus_tag="VFQ61_14295"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9955680.1"
                     /translation="MANATLNGRVLDSAGLPIQNALLVLHSSISEAPASVREASTDER
                     GVFRVRGVPRHANVTLNVWARGFEPRRDVLAHLETHGSLEVSLRRLPPLHAQVLDPKG
                     DPLPSAVVVACTGRTRVELVSGVDGNLTLSPDAVDCLATAYHPRFAHSESVRVGAQGT
                     VYFVMKPGGTIEGFASDPSGGSLPGLEFTIDSFEPAMGELPVWDLSSFPAEPIPRAFK
                     LESLCPGVYTLSFRHRARSPSTITLHATQISGIVVRADTVTRGLHVVVGRTPIMPETG
                     NEPGEPGPALEVTTSAEEETPDPGEPESAASEIERANEETAATE"
     gene            1378..2112
                     /locus_tag="VFQ61_14300"
     CDS             1378..2112
                     /locus_tag="VFQ61_14300"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HET9955681.1"
                     /translation="MRTPEGWRWIGESRHGKLEPPVFEDLLPADILTAWGDPSEPATE
                     VLPEEDQCVARAVAKRRNEFRKTREYARRLLREFGVHDFALLPGLKREPVWPEGIVGS
                     VTHTDGFCAVAIGPAARYAGIGIDAEPNLPIQPDVARHVCLPEEYEQHGWISPLLAAR
                     LIFSAKESIYKSQFYLTRQYLGFFDVKVQIHENGSFVGRLLVDAGPLRRGAELKGAFR
                     VRDGLLVTSACLRQGAESSVAPATLD"
     gene            complement(2070..3116)
                     /locus_tag="VFQ61_14305"
     CDS             complement(2070..3116)
                     /locus_tag="VFQ61_14305"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HET9955682.1"
                     /translation="MNQPSAALDRMVPQRRFPKFDPFTLALFGCAVLGHLVPVRGAGA
                     RVADHVLDAGIFSVFFLNGARLSRAELWAGLRQTKLQLSVLLSTFVLFPVLGLVLRLA
                     TARLTTPELTTGLMFLCTLPSTVQSSIALTALAGGNIPAAVCAAATSNVLGVFATPLL
                     VGLLGLGHVGAGSLTGSIWGVVMQLLLPFGLGHASRFWTGAWVARQKKLLSWVDRATI
                     LLVVYTAFSRARIEGLWQATPTTALLGTLCLVGVLLVCALTLTTLLARRLGFRLEDEV
                     TLVFCGSKKSLASGVPIARVLFGAQAGSLGALVLPIMLYHQLQLFACAWLAQRYRRRL
                     DQSRVAGATLDSAP"
     gene            complement(3130..4383)
                     /locus_tag="VFQ61_14310"
     CDS             complement(3130..4383)
                     /locus_tag="VFQ61_14310"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET9955683.1"
                     /translation="MQGIYVFETAIGPCGIAWGPRGITGIQLPEASQKATLARLARRF
                     PKAEPSKPPPHVASAVTWIRGLLAGKAEDLSRIELDFADVSPFYRRVYEFARSIPPGT
                     TLSYGDLAARMGSAGSARAVGQALGKNPFAIVVPCHRVLAAGGKLGGFSAHGGTQTKQ
                     RLLELERDLMKREPSAPKTPEPSLRRPGTLGAFRFDVDAAVAELCANDRALARWIERI
                     GPFEMRVEETASLFMALARSIVYQQLSGKAAGTIFGRFCGLFPGSPTAILPALVDQKT
                     DAELRGVGLSRNKLLALRDLSAKAQAGVLPSVEAAHEIPEEELIERLTQVRGIGRWTV
                     EMLMMFRLGRPDILPLDDLGIRKGFQVAFKTPELPARAQLEKRGERWRPYRTVASWYL
                     WRIAEFEMTRSAAKKSPVKKTPAKP"
     gene            4621..5853
                     /locus_tag="VFQ61_14315"
     CDS             4621..5853
                     /locus_tag="VFQ61_14315"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9955684.1"
                     /translation="MILLSSFLLRPVRRATVYSVCGALALTAPALTAPAWAQPARAAR
                     PSQSSPVPSSEGDRSVSPASSAGGAPAAVSSKEGPTPAAVSGQAAPSDPDVAARREHA
                     KLHYTQGVEAYRQGQYQAAVDHLLEASKLIQSPALSYNIAVSYAAMQDERAALRWFRN
                     YLRESPQAPDAASVAQRIEHLERALQGKGVQQVTFISDPPGATLELDERPIGVAPFTL
                     EIVPGNHRIRALLRGYSAEESVFELRPDRARDVRLELKPEPSVLAANATGAPAAAASN
                     HLDSSAKVAQPPAVPEPSRPAAASTRVKPSPARWLGIASVGVGGAALGAALAFELMRR
                     SAEDDAREASQAAYQDRYDTMNSRKTTARVFLGAGSAFLVGGLAALIVDWKMTQRRSV
                     GVATCGLQGWCPVAQGAF"
     gene            7025..8590
                     /locus_tag="VFQ61_14320"
     CDS             7025..8590
                     /locus_tag="VFQ61_14320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9955685.1"
                     /translation="MSKPSSDPRTVYQASSMDVPVISQTFRNYVEGDVIGGKYQLLRI
                     LGSGGMGAVWIARNRLLDIDVCLKLIRQGANELEHRDRLQREARAAARLNDPAIVRVL
                     DFGTTDLGEPYIVMELLQGSDLRTVLMERERIPPEEAVQLILPVIRAIGTAHDAGIVH
                     RDVKPENIFVAKTTSGALQPKLLDFGIARLDADENARLTKTGAVLGSPAYMSPEQARG
                     EVTDYRCDLWAIVLVLHEMLTGEATFDAPNYNAALQLILGKDPAPIDRFGIHEPVLSS
                     VLEAGLNKDAKRRFQSAAQLGRALADWLLSRGVMTDLTGLSLREGWFGRIRTPAVPEQ
                     TRASANRVRLEHSDTQLVGTAETAGARVQKRNWKLAVGIGVLVPISAIGVLLARAGLF
                     EKTSEPTHPAGTALVSAATDQTQLVAVPSNIPPPPPEAPLQTGPEVTLAPGAAEKGAR
                     DEQPVTNAKSGHAAPAASENAAPKSAPKSNSPAPQKNGAAATTSKSATSAAPPVTPAP
                     APAKDDSFKDPFE"
     gene            complement(8606..9712)
                     /locus_tag="VFQ61_14325"
     CDS             complement(8606..9712)
                     /locus_tag="VFQ61_14325"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HET9955686.1"
                     /translation="MLGSLRAFAESAPVTALAPMQYITDAPFIRTIAHYGAPDLVFTE
                     YFRVHAQSRLERHILETIAEHGEARPIFAQMIGEDPEALARTARELSRFAVAGIDLNL
                     GCPAPLIYRKNVGGGLLRDPEHVNRILGTLRQAIPGVFTVKMRIGFDSTQNFEPILKL
                     VSAHSVDLLTVHGRTVKEMYHATVHYAEIRRAVQQVSCPVLANGEVSSAEKARWVLHE
                     TGARGVMIGRHAIRNPWIFSQCRELFAGRAPMRPTLADVREYIERLYFATRAVDALER
                     SHVTKLKKYLNFVGLGVDPAGQFLHAMRRASSERELFTVCDEHLLREPTTPFAPEPFP
                     NLHARPRCEAGAETSGDVAAAEPSAELESCYASL"
     gene            complement(9720..>10722)
                     /locus_tag="VFQ61_14330"
     CDS             complement(9720..>10722)
                     /locus_tag="VFQ61_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET9955687.1"
                     /translation="ALEVGLLAGREVLTGHELSDLSDEELDLRIQRIGVFARVTPAQK
                     VRVVRALQRRGGVVAMVGDGANDAPAIRLANVGIAIGERSTQAAQAAADIVLTDERIE
                     SLAQAIWEGRAMWSSVRDAVSILVGGNLGEIGFTLGVGMIDGRPPLSARQLLLVNLLT
                     DVAPAMAIALRPPSVDSLSALAQEGPERSLGKPLNRDIVARASVTALGASTAWALGRV
                     LSGRSRAGTVGLVALVGTQLGQTLLSGNFSRSVIATSLASTVALGAIVQTPGLSHAFG
                     CRPLGPLGWTAAIGSSTAATLLANYFPGFVDQAMQRLRFDKPVFVEDPEALPATEGG"
BASE COUNT         1973 a         3413 c         3543 g         1793 t
ORIGIN      
        1 gacgtgcgcg accgcaagtc gcgacacctc ccaggcgcag gctctcgtcc acggcttccc
       61 ccgatccacc gcgcctgccc cccgaaataa cgccgtcgca acgtcgccac tccgacctga
      121 gcgtcacccc tattctgtgg cggccgtctc ctcgttggca cgctcgatct cgctggcggc
      181 actctccggc tccccgggat ccggggtttc ctcctccgcg gaggtcgtca cttcgagcgc
      241 ggggccgggc tcgccaggtt cgttgcctgt ctcgggcatg atcggggttc ttccgaccac
      301 cacgtgcagc ccgcgcgtga ccgtgtccgc gcgcacgacg atgccggaga tctgggtcgc
      361 gtgcagggtg atggtcgacg gcgagcgggc ccggtgacgg aagctcagcg tatatacgcc
      421 ggggcacaag ctctcgagct tgaatgctcg cgggatgggc tcggcgggaa agctcgagag
      481 atcccagacc ggcagttcgc ccattgcggg ttcgaacgaa tcgatcgtga actccaagcc
      541 cggcaagctg ccgccgctcg gatcactggc gaacccttca atcgttccgc cgggcttcat
      601 cacgaagtag accgtgccct gggcgccgac gcgcacgctc tcggaatgcg cgaaacgcgg
      661 gtgataggcg gttgctaggc aatctaccgc gtcgggtgag agggtgaggt tgccgtccac
      721 tcccgagacc aactccacac gcgtgcggcc cgtacaggct acgaccacgg ccgagggcaa
      781 tgggtcgcct ttgggatcca acacttgcgc atgcaatgga ggcaagcgcc gcagcgagac
      841 ctcgagcgaa ccgtgagtct cgaggtgagc gagcacgtcg cgacggggtt cgaaacctcg
      901 cgcccacacg ttcagggtca cgttcgcgtg gcgcggaacg ccgcgcactc ggaagacccc
      961 acgttcatcg gtcgaagcct cacggacact tgccggcgct tcgctgatgc tcgaatgaag
     1021 caccagcaac gcgttttgaa tgggcaaccc tgccgaatcg agcacgcgtc cattcagggt
     1081 cgcattggcc atgtccggcg aatcgggctt tgaccgggca aacgcgggcg tggccgctcg
     1141 atgggggggc gtctctctct cggctcggcc actgctgcgc ggacggtgct cagggctgtt
     1201 cgggcgagcg caaccggctc cgctgaccat caacgagagc aagagcggaa gtagcaggag
     1261 cacgcggggt agtcgcacga ggcgtctgac aagttggttg cacgcaggtt ccagctcgac
     1321 gttctcagcg cgtgcgaaga catacgcggc accactcgag gtacgcacca caagagagtg
     1381 cgtacccccg aaggatggcg atggatcgga gagtctcgtc atggcaagct cgaacctccc
     1441 gtgttcgagg atctgttacc cgcagacata ctgacggctt ggggggaccc gagtgagccc
     1501 gcgacggaag tgctgcccga ggaggatcaa tgcgtggccc gcgcggtggc aaagcgccgg
     1561 aacgagtttc gcaagactcg ggagtacgca cggcgcttgc tacgtgagtt cggcgtgcac
     1621 gatttcgcgt tgttgcctgg gctcaagcgc gaaccagttt ggccggaggg catcgtgggt
     1681 agcgtgaccc acacggatgg cttctgtgca gtcgcgattg gacctgccgc gcgctacgca
     1741 gggatcggta tcgacgccga acccaacctg ccgatccagc cggatgtcgc acgtcacgtg
     1801 tgccttccgg aagagtacga gcagcacggc tggatctccc cgttgctcgc ggctcggctg
     1861 attttctcgg ccaaagaatc aatctacaag agtcagttct acctgacacg tcagtatctg
     1921 ggtttctttg acgtgaaggt gcaaattcac gagaacggct cgttcgtcgg ccgcttgttg
     1981 gtcgatgcgg gcccgttgcg gaggggcgcc gaactcaagg gcgctttccg ggttcgagac
     2041 ggcctgctcg tgacgtccgc gtgtctgcgt cagggcgcag aatcgagcgt agctccggcc
     2101 accctcgatt gatccagccg gcgccggtag cgctgcgcga gccacgcgca cgcgaacaat
     2161 tgaagctggt gatagagcat gatcggtaac accagcgccc ccaggctccc cgcttgcgcc
     2221 ccgaacaaga ctcgagcaat gggcacgccg ctcgccaggc tctttttgga gccgcaaaac
     2281 accaaagtga cctcgtcttc gagacgaaag ccgagtcgac gtgcgagcaa ggtggtcagc
     2341 gtgagtgcgc agacgagcag aacgccaacc aggcacaggg taccgagcag ggccgtggtt
     2401 ggcgtggctt gccacaaacc ctcgatgcga gcgcggctga aggctgtata gacaacgagc
     2461 aggatcgtgg cgcggtctac ccaggacaac agcttcttct gtcttgcgac ccaagctccc
     2521 gtccaaaagc gcgaagcgtg gcccaagcca aacggcagca acagctgcat cactacgccc
     2581 cagatgctgc ccgttagaga gcccgcgccc acgtgaccca ggccgagtag ccccaccagc
     2641 agcggcgtcg cgaatacccc gagcacattc gaggtggcgg ccgcgcagac ggcagcaggg
     2701 atgttaccgc ccgccaacgc cgtcaaagcg atggacgact ggaccgtgga agggagcgtg
     2761 cacaagaaca tgagcccggt ggtcaattcc ggcgtggtga gccgagcggt ggccaagcgc
     2821 aggaccaaac ccaacacggg gaaaagcacg aacgtgctga gcagcacgct gagctgcagc
     2881 ttcgtctgac gtaggcccgc ccacagctcc gcgcgggaca gtcgcgcgcc gttcaggaag
     2941 aacacgctga agatgccagc atccaggacg tgatctgcga ctcgtgctcc tgcgccgcgc
     3001 acgggcacga gatggccgag cacggcgcaa ccgaagagtg ccaacgtgaa gggatcgaac
     3061 ttcgggaaac gccgttgggg caccattcgg tcgagtgcgg cgcttggctg attcacggct
     3121 acgctcgatt catggctttg cgggcgtctt cttgacgggc gacttctttg cggcagatcg
     3181 cgtcatctcg aactccgcga tgcgccacag gtaccagctt gcgaccgtgc gatacggccg
     3241 ccagcgctcg ccgcgcttct ccagttgagc ccgcgctggg agctcgggcg tcttgaacgc
     3301 cacctggaag cctttgcgaa tgcccaggtc atcgagggga agaatgtctg ggcgacccaa
     3361 gcggaacatc atcagcattt cgaccgtcca gcggccaatc ccgcgcactt gggtgagccg
     3421 ctcgatcagc tcttcttcgg ggatctcgtg cgcagcttcg acgctgggca gtacacccgc
     3481 ttgggctttg gcactcaagt ccctcagcgc gagcagtttg ttgcgagaca agccaacccc
     3541 gcgtaactcc gcatccgtct tttgatccac cagcgcgggc aaaatcgcgg tcggggaacc
     3601 gggaaacaga ccgcaaaatc gcccaaagat cgtgcccgca gctttgcccg aaagctgttg
     3661 gtacacgatc gatcgtgcga gcgccatgaa caggctcgcc gtctcttcga cacgcatttc
     3721 gaagggccca attcgctcga tccagcgcgc cagcgcgcga tcgttcgcgc agagctcggc
     3781 aacagcggcg tccacgtcga agcggaacgc tcctagcgtg cccggacgac ggaggctcgg
     3841 ttcgggagtt ttgggtgcac tgggctcgcg cttcatcaag tcacgctcga gctcgagcaa
     3901 tcgctgcttg gtttgggtgc caccgtgcgc cgaaaagccg cccagcttgc cgcccgcggc
     3961 cagtacgcga tgacaaggca cgacgatcgc gaacgggttc ttccccaacg cttggcccac
     4021 ggcgcgagcc gaaccggcgg aacccatgcg cgccgcgaga tccccgtagg agagcgtcgt
     4081 gccgggcggg atgctgcgcg cgaactcgta cacgcggcga taaaacggag agacgtctgc
     4141 aaaatcgagc tcgatgcgcg ataggtcctc ggctttgccg gcgagtagcc cccgaatcca
     4201 agtcactgca ctcgctacgt gcggcggtgg tttgctcggc tcagctttcg ggaagcgccg
     4261 ggcaaggcgc gccaaggtag ctttctgact cgcctcaggt agctggatcc cggtgatgcc
     4321 gcgaggtccc caagcgatcc cgcacgggcc aatcgcggtc tcgaaaacat aaatgccttg
     4381 catcgtcagc gcgtcccacg atggctgcga acgcgtgctc gggcaagaag accgtcatcc
     4441 ggcacacagc gcaatagccc cccgcgtccc tggtgtggta gttcgcagcg aaactgcgcg
     4501 taaatgggtt cttcggcacg tggaccggcg cccacgcgcc aaattgcggc gcgcgcggag
     4561 ggcttggcgc agctccggga ctaggtgcaa actgaggcgt tcgccgtcgg gccttaccgt
     4621 atgatcctat tatcttcttt tttgctgcgg cctgttcggc gcgccaccgt ctactccgtt
     4681 tgcggcgcgc tcgcgctgac cgcccctgcg ctgactgctc ctgcatgggc gcaacctgcg
     4741 cgagctgctc ggccgagcca gtcgagtccg gtcccctcga gtgagggcga tcgctcggtc
     4801 tcgcctgcct cttccgcagg cggcgctcct gcggccgttt cttccaaaga agggcctacg
     4861 ccggctgcgg tttcggggca ggctgcgcca agcgatcctg acgtcgcggc gcgccgcgaa
     4921 cacgccaagc tgcactatac gcaaggcgta gaagcttatc gccagggcca gtaccaggcc
     4981 gcagtggacc acttgttgga ggcgtccaag ctgatccaaa gccccgcgct ctcttacaac
     5041 atcgccgttt cctacgcggc aatgcaagac gagcgcgccg ctttgcgttg gtttcgcaac
     5101 tatctgagag agtcgccgca agccccagat gcggcttcgg ttgcgcaacg catcgagcac
     5161 ctcgagcgcg ccttgcaggg taaaggcgtg cagcaagtga cgttcatttc ggacccgccc
     5221 ggcgcaacct tggagctgga cgagcgcccc atcggagttg ccccgttcac gctggaaatc
     5281 gttcccggta atcaccgaat tcgagcgctg ttgcgcggct acagcgcgga ggaatccgtc
     5341 ttcgagctgc ggcccgatcg agctcgtgac gtcaggctcg agttgaagcc cgagccttcg
     5401 gttctggccg cgaacgccac cggggcaccc gcggctgcgg cgtcgaacca cctggattcc
     5461 agcgctaagg ttgcgcagcc acccgcagta ccggagccct cgcgccccgc cgctgcgtca
     5521 acgcgcgtca agccgagtcc cgcgcggtgg ttgggcatcg cgagcgtggg cgtgggcggc
     5581 gcggcgctcg gcgcggcgct cgcgttcgaa ctgatgcggc gctccgcgga agacgacgcg
     5641 cgcgaagctt ctcaagccgc ctaccaagat cgctatgaca cgatgaactc tcggaaaacg
     5701 accgcgcgcg tatttctcgg cgcgggctcc gcgttcctgg tgggcgggct cgcggcgttg
     5761 atcgtggatt ggaagatgac tcagcgacgc tcggtgggag tcgcaacctg cgggctccaa
     5821 ggctggtgtc cagttgcgca aggagcgttc tgatgtggcg ctcattgaag tggggctcgc
     5881 tgggcgcgct attgcttacg gcttgcaaca gcgacctcaa gcgcggcgcg ctagatgagc
     5941 ggccctgccc atgcctctcg ggttacacct gcgacacgtc gcgcggtgaa cacggcgtat
     6001 gtgtgcgcac cggcgcggga ggaagctccg ggggcgtgac ttctacgggc gcgaacggtg
     6061 gcaccgccgg tctctcgaat ccggggggcg gctccgctac ggacaaagga gggacgtcct
     6121 cacgtggcgg gcaaacggct gccggtcagt ccgccggagg atcgtctggc gacgctccac
     6181 aaggcggtga aacggctgcg ggcggcacag agccggctgg cggaaccgga gggagttcgg
     6241 gcgggcaacc cccggagacg ggtggacaac cgagcggagg cacatcactg ggtggacaag
     6301 ggccgaacgg gggatcttcc gcaacgaccg gagacggcgg aagaacgacc tgttctgtcg
     6361 acgtctttcc taccgcgcct ggtgattgcc cgactgaatg cgagcgttgt agcgaaggtg
     6421 tctgttactt caggtgcgat gcgaccaccg gctgcgaaca aagcaccatc gcttgtccga
     6481 ccggctttgc ttgccacgtt ttatgcgaaa gccagcgagc ttgtcggaat acggtggtcg
     6541 agtgtccaca cacctatggt tgcacgctcg aatgccaagg cgtgcaggct tgtcaaaggg
     6601 cggcgctcaa ctgcagctca gatgggcctt gctcgctaaa gtgcggcagc caaacgcaaa
     6661 gttgtcagaa tgcgaaagtc aggtgtggag tcgatgcctg cgaaacgtcg tgctcgatgg
     6721 gtgataccgt ccaggttgac tgcaccccga cctgcagcgg cagttgtggg tgttccttgc
     6781 cttagtattc agtgcgacag gagaacgggg ctaaaggcga ctccgctggt caccaggcca
     6841 acgggctggc tcgcccgacg ccaatcgcac gcgcgcggca cgagtcaacc gcctcgcctt
     6901 ggcgggctag cgcgataaga tgacgggccg aaccggcggt gaggaagccc ccgagagaac
     6961 ccggtagcca gcagaaccgc cccatgcgca tcaggacacc tgaagacttg gggaaccgct
     7021 gaccatgagc aaaccttcga gcgacccacg taccgtctat caagcctcct ccatggatgt
     7081 gccggtcatt tcgcagacgt tccggaacta cgtggagggg gatgtaatcg gcgggaagta
     7141 ccagctgctc cggatcttgg gcagcggcgg gatgggtgcg gtttggattg ctcgaaaccg
     7201 gctcttggat atcgacgttt gtttgaagct gattcgccag ggggcgaatg agctcgaaca
     7261 tcgcgaccga ctgcagcgcg aggcgcgcgc cgcagcgcgt ctgaacgatc cggcgatcgt
     7321 gcgggtcttg gatttcggca ctaccgactt gggagagccg tacatcgtga tggagttgct
     7381 gcaaggcagc gacttgcgca cggtgttgat ggagcgagaa cgcattccgc ccgaagaggc
     7441 ggtccagctg attttgccgg tgatccgcgc gattgggacg gcgcacgatg ccggcatcgt
     7501 gcatcgcgac gtgaagccgg agaacatctt cgtggccaag accacgagcg gcgcattgca
     7561 gcccaagctc ttggatttcg ggattgcccg actcgacgcg gatgagaacg ccaggctgac
     7621 gaagacggga gcggtgctcg ggagccccgc gtacatgtcg ccggaacaag cgcgcggcga
     7681 ggtgacggac tatcgctgcg acctgtgggc gatcgtgctc gtgttacacg agatgctgac
     7741 gggtgaagcc acgttcgatg cgccgaatta caatgccgcg ttacagctga tcttgggcaa
     7801 agatcccgcg cccatcgacc gcttcgggat tcacgagcca gtgctgtcga gtgtgctcga
     7861 ggccgggctc aacaaggacg caaaacggcg cttccaaagc gctgcccagc tcggtcgcgc
     7921 gctggcggat tggctgttga gccgcggcgt gatgacggat ctgaccggcc tatccttgcg
     7981 cgaaggatgg tttgggcgca tccgcacacc ggccgttccg gagcagactc gagcgagtgc
     8041 caaccgggtg cggctggagc attccgatac ccaactcgtg ggaaccgcgg agaccgccgg
     8101 ggctcgcgtg cagaagcgga actggaagct cgcggttggg attggcgtgc tcgtgccgat
     8161 ttcggcgatt ggggttttgc tggcgcgggc gggcttgttc gaaaaaacca gtgagccgac
     8221 gcatcctgct ggaacggcgc tcgtgtcggc cgcgaccgat cagactcagc tggtggccgt
     8281 gccgagcaat ataccgcctc cgccgccaga ggcgccgctc cagacgggtc cggaagttac
     8341 cttggccccg ggcgcggcag aaaagggcgc tcgcgatgag cagcccgtaa cgaacgccaa
     8401 aagcggccac gctgcgccgg ctgcgtcgga aaacgcggct ccgaaatcgg caccgaagtc
     8461 caactcgccc gcgccgcaaa agaacggggc cgcggctacg acgtcgaagt ctgccacttc
     8521 ggcagcgccg cccgtcacgc ccgcaccagc tccggcgaag gacgattcgt tcaaagatcc
     8581 gttcgaataa gcgaacaaat cgacgtcaga gactggcgta acagctctcg agttcagcgc
     8641 tgggttcagc cgcggcgacg tcgccgctgg tttccgcgcc tgcttcgcag cgtggacgcg
     8701 cgtgcaaatt agggaacggc tcaggcgcga acggcgtggt cggctcccgc agcaaatgtt
     8761 cgtcgcagac cgtgaagagc tcgcgctcgc tactcgcgcg acgcatggcg tggaggaatt
     8821 gcccggccgg gtccacgccg aggccgacga agttcaggta cttcttcagc ttggtgacgt
     8881 gggagcgctc cagcgcgtcg acggcgcgag tcgcgaaata cagccgctcg atgtactcgc
     8941 gcacgtccgc gagcgtaggc cgcattgggg ctcgtcccgc gaagagctcg cggcattgcg
     9001 agaatatcca gggatttcga atcgcgtggc gcccgatcat cactccgcgc gcgcccgttt
     9061 cgtgcaaaac ccaacgggct ttttcggcgg agctgacttc gccgttggcg agtacggggc
     9121 aagacacctg ttgcaccgct cggcgaatct cggcgtagtg cacggttgca tggtacatct
     9181 ccttcaccgt gcgcccgtgc accgtcagga gatccacgga gtgcgcgcta accagcttca
     9241 ggatcggttc gaagttctgg gtggagtcga agccaatgcg catcttcacc gtgaacaccc
     9301 cggggatggc ctggcgcagc gtgcccaaga tccgattgac gtgctcgggg tcgcggagca
     9361 ggccgccgcc gacgtttttg cggtagatga gcggcgcggg gcaccccaaa ttcaaatcga
     9421 tgccggccac ggcgaagcgt gagagctcgc gtgcggtgcg cgcgagtgct tcgggatctt
     9481 cgccgatcat ttgggcgaag attggccgag cttcgccgtg ttcggcgatc gtctcgagga
     9541 tgtggcgctc gagtcgagac tgcgcgtgca cgcggaagta ctcggtaaac accaagtctg
     9601 gcgcgccgta atgcgcgatc gtccgaatga acggcgcgtc ggtgatgtat tgcatcggcg
     9661 cgagcgccgt gaccggcgcg ctctcggcaa aggcgcgcag cgagcccaac atgccgcggc
     9721 tacccgcctt ccgtggccgg caacgcctcc ggatcttcca cgaacacggg tttgtcgaag
     9781 cgcaagcgtt gcatggcctg gtccacgaat cccgggaagt agttggcgag caacgtggcg
     9841 gccgtggacg aaccgatagc ggcggtccag ccgagcggac caagcggacg gcagccaaat
     9901 gcgtggctca gccccggcgt ttgcacgatg gcccccagcg cgaccgtgga cgcgaggctg
     9961 gtcgcgatca ccgagcggct gaaattgccc gacaacaacg tctgaccgag ttgggtcccg
    10021 acgagcgcga ccaggcccac ggtaccagcg cggctacgcc cggagagcac gcgcccgagc
    10081 gcccaagcgg tgctcgctcc gagcgcggtg accgacgcac gggccacgat gtcgcgattc
    10141 aacggcttgc cgagcgagcg ctccgggccc tcttgggcga gcgcgctcag cgagtccaca
    10201 ctgggtggcc gcaacgcaat cgccatcgca ggtgcgacgt cggtcaacaa gttcacgagc
    10261 aacagctgcc gggcgctgag cggcgggcgg ccatcgatca tgccgacgcc caaggtgaag
    10321 ccgatttctc ctaggttgcc gccgaccaag atcgaaaccg cgtcgcggac cgaggaccac
    10381 atcgcgcggc cctcccaaat cgcctgcgcc aaggactcga tgcgctcgtc ggtgagcacg
    10441 atgtcggccg cggcctgtgc ggcctgagtg ctgcgttccc caattgcgat gcccacgttc
    10501 gccaagcgaa tcgcgggcgc gtcattggcg ccatcgccca ccatcgcaac caccccgccg
    10561 cgccgctgca gcgcgcgcac cacgcgcacc ttctgcgccg gggtgacgcg ggcgaacacg
    10621 ccgatgcgct ggatgcgcaa atcgagctct tcatcggaca agtccgagag ctcgtgaccc
    10681 gtcagtacct cgcggcccgc gagcagcccg acctccagcg cc
//
LOCUS       DASTKU010000417         4779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_969814, whole genome shotgun sequence.
ACCESSION   DASTKU010000417 DASTKU010000000
VERSION     DASTKU010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969814"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..1503
                     /locus_tag="VFQ61_14335"
     CDS             244..1503
                     /locus_tag="VFQ61_14335"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9955688.1"
                     /translation="MTTHTSALRPGIEERLNVMEASAAALKSHGLIHRVAEDARLDGR
                     RISVDGRALVNFGSCSYLGLETDARLKTAACEALLRFGTQFSSSRAYVSLPLYAEFEA
                     LLSELVGGYPVVVSQTTSLGHLAALPVLVGANDAVLFDVQVHSSLQAVLPTLRMAGVP
                     CEAVPHNRLDRLEERARALAERHARVFYLCDGVYSMHGDAVDVNGLCGVLDRIPNLHA
                     YVDDAHGVGWSGARGSGHVLGAGPLHPRMMVILGLSKSFGAAGAALVFPTRELAERVF
                     TCGSTLIFSGPLQPAQLGAGVASARIHLSPELEQLQAGLRQRIQCFDACATEQKLRTR
                     SQIQTPIRFVEVGSEEVAMNWGGALKRAGFFTNVAVYPAVPRRRAGIRLMLNLNQTLD
                     DVRGVVHELGRLTSKASSTIQAAPALT"
     gene            complement(1576..2337)
                     /locus_tag="VFQ61_14340"
     CDS             complement(1576..2337)
                     /locus_tag="VFQ61_14340"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9955689.1"
                     /translation="MILPGRLAATTLGDLLGQLHRGRVTGILELLEDSGSASGQLHRI
                     FLNEGLVDAAVTPLPAPRLGELLLCEGALTRAGLLQLSRLLIERPCARAGRLLLEQRL
                     SSPSAVRTGLHRQLRLRVDALFHLRHARVRFHVRAPAPEPDVDPLDAQEFLHGRPRAR
                     ADRKRDEPAQNAGHEGQPFTSREQIHTSDQRAALRVLGLPAGADLGDIQRAFRLSARL
                     FHPDRHPRATAQERAELLRHFAELSHAYHSLLRSA"
     gene            complement(2426..2977)
                     /locus_tag="VFQ61_14345"
     CDS             complement(2426..2977)
                     /locus_tag="VFQ61_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955690.1"
                     /translation="MSCFKHPSTLRGPVARRAALGALSVLAAVLPIEVRAQAFPSGAA
                     APSAPAPPQSNGIQPSSGGAATASRLGATLNCPPAQGAQRVVCALRVEAPAGYQISWV
                     DALVLDTPAFAGVLRSRVWARNARSSVAVEVPLALVALSPGSGWMTVRARAVLCPREG
                     TGRCRPFTREVRAEVVVEGTPRG"
     gene            complement(3001..4128)
                     /locus_tag="VFQ61_14350"
     CDS             complement(3001..4128)
                     /locus_tag="VFQ61_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9955691.1"
                     /translation="MESPQSNVPATTAPESVPPAPPTASSVLPSSPSPEPPRIAVVVN
                     GRAKNVTAEVISTLGQILSGGDLFVSRSLSDAKEIAKTLVQRRYGTVLTGGGDGTFTV
                     MVTEVVHEARRTGRPLPRFGLLKLGTGNSLAWVVGAGEVKGGELDADIEHLAQHAGSR
                     TLRLIEVEGIISPFCGLGADARVLADYGAVKERLSRGRLGRRLAPGAVSYTVAAVTRS
                     LPQQVLGRMPHCRIVNAGEPVLRMGAKGSVVGRPIERGEVVYEGPARLAAMSTIPYYG
                     YGFRMFPYAEERPDRMHLRVATISPLQFVRHFAEIWRGSFEDPGSVFDYLVEAIDIEL
                     DPPTPFQIGGDSRGDRSKLHVALCKNPVELVDLYAPPSVPA"
     gene            4218..>4779
                     /locus_tag="VFQ61_14355"
     CDS             4218..>4779
                     /locus_tag="VFQ61_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955692.1"
                     /translation="MVKSTAKRRSASPAAERATAQSEPARVTKQSVPPPSLSGANAEA
                     RSTARPLKAGKPQSVRASGGPAPGSREAAQQQAELDKSKADAGAEAAGPRRFRGAAPW
                     AARHAAKRAAEFAARNQEPPRPGSARATLRTPEQAESIKARVTELHQLLNSLRSTKKH
                     LKERFLEAGELLRRLRDEKLFEAKGYS"
BASE COUNT          879 a         1565 c         1578 g          757 t
ORIGIN      
        1 cgcgcagttg ggaaccgacg gcgcccggca cgagctgagt cttcggctcg cctgcggagc
       61 ccggaacggc agcctctcgc gcgctccgct ccggcggagc catgcaccga gccctaaccc
      121 gcacggatgg gggagtaatc gaggtaccca cccaaatgaa cggcgtgacc taagtcgcat
      181 gttgctcggc cgttgctccc cgagagtcga agcgggggca accgaaacag tcgaggacaa
      241 cgtatgacca ctcacacgtc cgctttgcgt cccgggatcg aggaaaggtt gaatgtgatg
      301 gaggcgagcg cggcggcgct caagtctcac ggcttgatcc atcgcgtcgc cgaggacgcg
      361 cgcttggacg ggcgtcgaat cagcgtggat ggccgcgccc tcgtcaactt tggcagctgt
      421 tcctatctgg ggctcgagac cgacgcacgc ctgaagacgg ccgcctgcga agccttgctg
      481 cgcttcggca ctcagttctc gtcttcgcga gcatacgtat cgttgccgct ctacgccgag
      541 ttcgaggcgc tgctgagcga gctcgtcggc ggctaccccg tggtggtcag ccaaacgaca
      601 tcgctcggac atttggcggc gctgcccgta ttggtcggcg caaacgacgc ggtgttgttc
      661 gacgtgcaag tgcacagcag tctgcaagcc gtgctgccga ccctgcgcat ggcgggcgtg
      721 ccgtgcgaag cagtgccgca caatcgcttg gatcgtctgg aggaacgagc tcgtgccctt
      781 gctgagcgcc atgcacgcgt cttctatctg tgtgacgggg tctacagcat gcacggcgac
      841 gccgtggacg tgaacgggct atgtggtgtt ctcgatcgaa ttcccaacct acatgcctac
      901 gtcgacgatg cccacggcgt aggttggtcg ggtgcgcgtg ggtcgggcca cgtgttgggc
      961 gcaggcccac tgcacccgcg catgatggtc attttgggac tgtccaagag cttcggagcc
     1021 gccggcgcgg cgctcgtgtt tccgactcgg gagctggcag agcgggtgtt cacctgcggt
     1081 agcacgctga tcttctcggg cccgctgcag ccggcccagc tcggagctgg ggtggcctcg
     1141 gcgcgaatcc acctgtcgcc ggagctcgag caactccagg cggggcttcg gcagcgtatt
     1201 caatgcttcg acgcctgcgc aacggaacag aagctccgca cgcggtccca gattcaaacc
     1261 ccgattcggt tcgtggaagt aggctccgaa gaggtcgcca tgaactgggg tggcgcgttg
     1321 aagcgagccg gcttcttcac caacgtagcg gtctatcccg cggttccccg ccgacgggcg
     1381 ggcatccgct tgatgttgaa cttgaaccag accctagatg acgtgcgcgg agtagtccac
     1441 gaactcgggc gtctcaccag caaggcctcg agcaccatcc aggccgcgcc tgctctaact
     1501 tagcgcctat cccaatctca ggaccgcaca catttttgtt gaaaaagatt gggatagttc
     1561 ttcgccgcag ggcaatcagg cgctgcgcag cagcgagtga taggcgtggc tgagctctgc
     1621 gaaatggcgc agtagctcgg cccgctcctg ggccgtggcg cgcgggtgac ggtccggatg
     1681 aaacagccgc gcgctgagcc gaaaggcgcg ctggatgtcg ccgagatcgg cgcccgccgg
     1741 caaaccgagc acccgcagtg cggcgcgctg atcgctggtg tggatttgct cgcgactggt
     1801 gaagggttgc ccttcgtggc cggcattttg cgcgggctcg tcgcgcttgc ggtcggcgcg
     1861 cgcgcggggc cgtccgtgca ggaactcctg cgcatcgagc ggatcgacgt cgggctcggg
     1921 cgctggcgcg cgtacgtgga agcgcactcg ggcgtgccgc agatgaaaga gcgcatctac
     1981 ccgcagccgc agctggcggt gcaagccagt gcgcacggcg ctaggagatg acagacgctg
     2041 ctctagcaac agacgccccg cgcgagcgca gggcctctcg atcagcaaac gcgagagctg
     2101 caacagaccc gcgcgcgtga gggcgccttc acaaagcagc agctcaccga gccgcggggc
     2161 gggcagcggc gtgacggccg catcgaccag accctcgttg aggaagatgc gatgcaactg
     2221 cccggaggca gagcctgagt cttcgagcaa ctcgagaatg cccgtaaccc gacctcgatg
     2281 gagctgtccg aggaggtcgc cgagcgtcgt cgcggcaagt cgacccggca agatcatgct
     2341 gcacggttaa caagagctag cccgactggc caagtttcgg cccagatcca cggccacaac
     2401 cagcgcgcac agcacagtgc gtcgctcagc cgcgaggagt accttcgacc acgacctcgg
     2461 cccggacctc gcgcgtgaac ggtcggcagc ggccagtacc ttcacgcggg cacagcaccg
     2521 cgcgcgctct caccgtcatc caacctgaac cggggctcag tgcgacgagg gccagcggaa
     2581 cctccaccgc aacgctcgat cgggcattac gcgcccagac cctggatcgg agcacccccg
     2641 cgaacgctgg cgtatccaac accagcgcgt ccacccagga aatctggtag cccgcagggg
     2701 cttccacgcg caacgcgcag accacccgct gcgcaccctg agcgggtgga cagttgagcg
     2761 tcgcgccgag cctggaagcg gttgcagccc cacccgagct cggctggatt ccgttggatt
     2821 gtggcggtgc gggcgctgac ggtgcggctg cgccgctggg gaaggcctgg gcccggacct
     2881 cgattggcag caccgcggcc aagaccgaaa gcgcgccgag tgctgcccgt cgagcaacag
     2941 gaccacgcag cgtcgaggga tgtttgaagc aggacatgag aaccgtacgc cacgctcgag
     3001 tcaggcggga acgctcggcg gcgcgtaaag gtccacgagt tccacggggt tcttgcacaa
     3061 cgctacatgt aatttcgagc gatccccgcg tgagtcccca ccgatctgga agggcgtggg
     3121 aggatcgagc tcgatgtcga tggcttccac caagtagtcg aaaacggatc cggggtcttc
     3181 gaagcttccc cgccagattt cagcgaagtg ccggacgaac tgcagcggcg agatcgtcgc
     3241 gacccgcaaa tgcatgcgat ccggacgctc ctccgcgtat ggaaacattc gaaacccata
     3301 gccgtaatac ggaatcgtcg acatcgcggc caagcgagcc ggcccctcgt agaccacctc
     3361 cccgcgttcg atgggacgcc ccaccacact gcctttggcg cccatccgca gcaccggctc
     3421 gcccgcattc acgattcggc agtgcggcat gcggcccaga acctgctgtg gcaacgagcg
     3481 ggtgactgct gcgacggtgt agctcaccgc gcccggcgca aggcgccgtc ccaatctccc
     3541 gcggctcagc cgctccttca ccgcaccgta gtcagcgagt acgcgcgcat cggcgccaag
     3601 cccgcaaaat ggcgaaataa tcccctcgac ttcgatgagg cgaagcgtgc ggctgccggc
     3661 gtgctgcgcc aggtgttcga tatcggcgtc cagctcaccg cccttcacct ctccggcgcc
     3721 gaccacccaa gccagcgagt tgccggtccc gagcttgagc aaaccgaatc gtggcaacgg
     3781 ccgcccggtg cgccgggctt catgaacgac ttccgtaacc attacggtga aggtcccgtc
     3841 cccgcccccg gtgagcaccg tcccgtaacg ccgttgcacc aaagtcttcg caatctcttt
     3901 ggcgtccgag aggctccgcg aaacgaacaa atcaccaccc gacaagattt gtccaagggt
     3961 cgagatcacc tccgcagtca cgttcttggc gcgaccattg acgactaccg cgatgcgcgg
     4021 cggttccggg ctcggtgaag acgggagaac cgacgaggcg gtgggcggcg ccgggggtac
     4081 ggattcaggc gcagtcgtcg ctgggacgtt ggactggggc gactccatga ttttggggtt
     4141 ccgagcgcgg agtatactag gctccggctc aggcgcttta tttggagcgc ggagcggttc
     4201 gccgcgagag ggttttcatg gtcaaaagca ccgccaagcg ccgttccgct agcccggctg
     4261 cggagcgtgc aactgctcaa tcggagccgg cccgggtcac gaagcagagt gtcccgccac
     4321 catcgctttc gggagccaac gccgaagcgc gctcaaccgc gcgaccgttg aaggccggta
     4381 agccgcaatc cgtcagggcc agtggagggc ccgccccggg gagcagggag gctgcgcagc
     4441 agcaagcgga gctggacaag agcaaggccg acgcaggagc cgaagccgca gggccacgtc
     4501 gttttcgggg cgcggccccc tgggcggcca gacatgcggc caagcgcgca gcggagttcg
     4561 ccgcgcgcaa tcaggagccg ccgcgccccg gtagtgcgcg cgcaacactg cgcacgcccg
     4621 aacaagccga gagtatcaag gccagagtta ccgagctcca ccagctgctg aattcgctgc
     4681 gctcgaccaa aaagcacttg aaggagcgct tcttggaggc aggtgagctg ctgcgccggc
     4741 tgcgcgacga gaagctgttc gaggccaagg gttacagct
//
LOCUS       DASTKU010000418         8282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_969846, whole genome shotgun sequence.
ACCESSION   DASTKU010000418 DASTKU010000000
VERSION     DASTKU010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969846"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VFQ61_14360"
     CDS             complement(<1..803)
                     /locus_tag="VFQ61_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955693.1"
                     /translation="MFARPQQLGHSKRFAQPAATLAIAAAPPLEDGLISADARNASRT
                     MSGGGRRGFSGLLGALGVGLVASLCWVTPAAAADETSDADTSADEKSDDKKEGEDANE
                     GADKETDGAAEAAAEAARAKAEADSTPVEKRGETYYFVGARYRLVVVPAFMMHLFADG
                     GTTVPVHSGGAEFGIRKDNFEYNLGLWLASYAMDPTKFKSKTDADDAWELVESKLKVL
                     YLTADFMWSQPVAKEFAINYGMGAGLGIVFGDLLRTQAYPDPNGPGGYSP"
     gene            1318..1752
                     /locus_tag="VFQ61_14365"
     CDS             1318..1752
                     /locus_tag="VFQ61_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955694.1"
                     /translation="MTLRSSIGLLMLWLTAGCGTHLVTRGADLYAGGYYIEAAEVFEH
                     SERRLPDYSTGERARYGLYRGATLLALGDVSRADNWLVYSAEIEKGHPSTLTDEERQM
                     LSRALELSVAHRTDRSRGVLQTPAKVAATAALPVNAHGVEQR"
     gene            complement(1925..4018)
                     /locus_tag="VFQ61_14370"
     CDS             complement(1925..4018)
                     /locus_tag="VFQ61_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HET9955695.1"
                     /translation="MDQSGTISRTNEPAAGSMVSLVASMQDFDRYRDLAWEGSFEEYL
                     QIVRERPQVTRNAFQRVYDMVISYGTEEYIDNKKKLVRYNFFRDEIEEGKDAVFGLDI
                     PLMRLMNVLKAAAEGYGPEKRVILLHGPVGSSKSTIARLLKKGLERYSQTTEGALFTF
                     KWVNLGESGLASGGADTFDSPMHEEPLRMIPQEWRERAIDKLGLSNDKFRVRIEGDMD
                     PASRFIFNGLLQRYQGDWGKVVSNHVRVKRLLLSEKDRVGIGTFQPKDEKNQDSTELT
                     GDINYRKIAEYGSDSDPRAFNFDGEFNIANRGIVEFVEVLKLDVAFLYDLLGASQEHR
                     IKPKKFAQTDIDELIIGHTNEAEYKRLLNNEFMEALRDRTIKIDIPYITRMTDEIRIY
                     EKDFNQEKVRGKHVAPHTLEVASMWAVLTRLEEPKKHNLALLQKMKLYDGKVLPGYTQ
                     DTVKELRKETKREGMEGISPRYVQDKISNALVSDVGEGTINPFMVLNELEKGLTHHSL
                     ITSEEQRKRYRELIGVVKREYEEIVKNEVQRAISADEEAIAKLAANYIDNIKAYTLKE
                     KVKNRYTGQDEEPDERLMRSIEEKIEIPENRKDDFRREIMNFIGAKAVEGKRFDWQTN
                     DRLRRALELKLFEDQKDSIKLKTLVSAVVDKETQEKIDIIKSRLMRYFGYNEVSATDV
                     LNYVASIFARGDAKG"
     gene            4652..6898
                     /gene="ppk1"
                     /locus_tag="VFQ61_14375"
     CDS             4652..6898
                     /gene="ppk1"
                     /locus_tag="VFQ61_14375"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HET9955696.1"
                     /translation="MSAPEPLRASLNLPSEIPVAVVPGRLDDPSLYFNRELSWLEFNA
                     RVLAEANDPQVPLYERLKFLAIFASNLDEFFMVRVAGLKAQLYGAVEEVSPDGMTPEQ
                     QLVAVVDRVHELMAEQYRIYTSALRPELAQAGVALIQPEELSTKEQAALDQHFIQNIY
                     PVLTPIAIDPVHPFPHIRNKAINVGVIFEKRLQSSEPSFGLVQVPTMLPRLIRVPREG
                     LRRAFVALEDVILRHTKTIFPQLKVIGQYAFRVTRNFDIEIDEEEGEDLLITLQAELR
                     RRERGHAVRLEVSGQAPRESLQWLCRELELESRDVYQAGGPLFIGDMTVLTSGDERRD
                     LRDEAFSPQYVPPLRDSEDFFETIKERDVLLHHPYESFEAVIDFLWQAAEDPRVLAIK
                     QTLYRTGGESPLVRALQRAAELGKHVTALVELKARFDEESNIQWARALERSGVNVMYG
                     LLGFKTHAKVLMVVRRERDRLRRYVHLATGNYNQQTARLYEDLSLFTAREEYGEDATA
                     FFNMLTGYSAPVRWNRWIVAPLGLHEAVLGLIARETEHALNGRPARIVAKMNSLVDAS
                     VIAALYAASQAGVEIDLLVRGICCLRPGLPGVSERIRVQAVIDRFLEHARIFYFQNGG
                     NAEVYCSSADWMPRNFRRRVEVMFPVVDDALKSRVIEEILGAMRGDRAKTWLLGESGA
                     YVRMGDATGLRSQQRFIDLARERARENEAILGLSKPVATPGGRALDKLRRRGGKKRKK
                     RNRGENDD"
     gene            7108..7635
                     /locus_tag="VFQ61_14380"
     CDS             7108..7635
                     /locus_tag="VFQ61_14380"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9955697.1"
                     /translation="MTSTSALKARAALGNRRSHGRAKLCVPVLLDTARSYFKAFSKDV
                     SESGIGIEVGGPVGAGNELEVGRCVELYFELPTGSGLELSAEVVRSEDDGIGLTFRGL
                     SPEASQALKHYCEQWKAELIQRCRTRASNDSSEPANASAASVARSFSARPIRAAQPAF
                     YDDSEMSSEIRIRYR"
     gene            complement(7673..>8282)
                     /locus_tag="VFQ61_14385"
     CDS             complement(7673..>8282)
                     /locus_tag="VFQ61_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955698.1"
                     /translation="IFVAWFAYFSLPAAYAFIWPIHARFPLLAMLLGVAVLPSISRRL
                     RWLVGATLTAVTLLTSWNLARLLREIERTEYAGFGSAIEAIPEGSHVVGLIYGARSAY
                     VRFAPYLHAVAWYQAERGGIVMFTFAEFPSSPFRFREHHRPPPVPERWEWLPQWVDTQ
                     RDLDFYDWAVVRGGPAVLPGFALHQRRGDWSVWRHIHEGVSE"
BASE COUNT         1555 a         2573 c         2535 g         1619 t
ORIGIN      
        1 ggggagtagc cgccgggccc gttgggatcc ggatacgctt gcgtgcgtag caagtcccca
       61 aagacgatgc cgagccccgc gcccatgccg tagttgatcg caaactcctt cgcgaccggc
      121 tggctccaca tgaagtccgc cgtcagatag agcaccttga gcttgctctc gacgagctcc
      181 caagcatcat ccgcgtcggt cttcgacttg aacttcgtcg gatccatcgc gtagctcgcg
      241 agccacagcc ccaagttgta ctcgaagttg tctttgcgga tgccgaactc ggccccgccc
      301 gagtgcactg gcacagtggt cccgccgtcg gcgaacaagt gcatcatgaa cgccggcacc
      361 accaccaatc gataacgggc cccgacgaag taataagtct ccccgcgctt ctcgactggt
      421 gtggaatcag cttccgcttt ggcccgcgcg gcctcggcgg ccgcttccgc cgcgccgtcc
      481 gtctccttgt cggcgccctc gttcgcgtcc tcgccctctt tcttgtcatc cgacttttca
      541 tccgcactcg tgtcagcgtc ggatgtttcg tccgctgccg cggccggtgt tacccagcac
      601 agggacgcga ctagacccac acccagagca ccgagtaatc cgctgaaccc tcgacggccg
      661 ccaccgctca tggtccgtga ggcgtttcgt gcatctgcgg agatcaggcc gtcttcgaga
      721 ggcggtgcgg ctgcaatggc gagcgtagcc gctggttgtg caaagcgctt ggagtgcccg
      781 agctgttgag gacgcgcaaa cattggaaaa cctccaagaa agagggctcg cacgtcattg
      841 gctcaactcg ggccacgggc gcgaaccgtt gagccatact ggcacggcaa tgtgccaaat
      901 ggcaaagcct tcgtagccga tcctcgcgct cttgggtctc tcccgagtcc gtcacaaacg
      961 gcaattgtca gcccgggcgc acgatcctcc gcgcatgcca ggggaaacgg ctacaccccc
     1021 actgagccaa ccgcacagtg ggtaacgcct tacgcaaagg ggatcctaat ccactgacgt
     1081 ggctgatgaa tcggtggtcc ttaacgaggt ttgtaagctc agcttggtaa cctcatcagc
     1141 gcgtaaagcc aaacacaggc acgggcacag tacggcgcag tacggcgcag atattgagca
     1201 tagaaaccgc acaggaacag agcccgtgaa gttgggtgcc ttacggttgc gcagaatgcc
     1261 cttcaaaata gagcttccga gtagcgactc gaaccctgca cagttaccat tcagctagtg
     1321 actttgcgtt cttcgatcgg cttgctcatg ctgtggctca ccgcgggctg tggcactcat
     1381 ctggtcaccc gcggcgcgga cctctacgcg ggtggctatt acatcgaggc agcggaggta
     1441 ttcgaacata gcgaacgccg cttgcccgat tactcgacgg gcgaacgcgc gcggtatggc
     1501 ctctatcgag gcgccacctt gctcgcgttg ggggatgtct cgagggccga caattggttg
     1561 gtttattctg ctgagatcga gaagggccat ccctcgacgc tcaccgatga agagcgtcag
     1621 atgctttccc gcgcgctcga gctgtccgtg gcacatcgca cggatcgctc gcgcggcgtg
     1681 ttgcagacgc cagccaaagt cgcagctacg gccgcgctac ccgtcaatgc gcacggcgta
     1741 gagcagcgct gacgcgcgca tcgagcccag gccgggctgg gaacacgacg ctccaccttt
     1801 gaatcacgcg ccagctcgat tcgcgggcgt tccgcagttc tccgagtgct cgaaacgact
     1861 ccgcccgact gcggaggtgt tccacagccg ggcggcttgg ggcatttggt gatgggccga
     1921 gcttttagcc cttcgcgtct ccgcgagcga agatcgaagc cacgtagttc aagacatcgg
     1981 tcgcgctgac ctcgttgtaa ccgaagtagc gcatgagccg agacttgatg atgtcgatct
     2041 tctcctgcgt ctccttgtcc accaccgcgg agaccagcgt cttgagcttg atgctgtcct
     2101 tctgatcttc gaacagcttg agctcgaggg cgcggcgcag gcgatcattc gtctgccaat
     2161 cgaagcgttt gccctcgacg gccttggcac cgatgaagtt catgatctcg cgccggaagt
     2221 cgtccttgcg gttctccgga atctcgatct tctcttcgat cgagcgcatc aagcgctcat
     2281 ccggctcttc gtcttggccg gtgtagcgat tcttgacctt ctctttgagg gtgtaggctt
     2341 tgatgttgtc gatgtagttg gcggcaagct tcgcgatcgc ctcttcgtcc gcgctgatgg
     2401 cgcgctggac ctcgttcttg acgatctcct cgtactcgcg cttcaccacg ccgatcagct
     2461 cgcgataacg cttgcgctgc tcttcgctcg taatcagcga atggtgggtc aggcccttct
     2521 cgagctcgtt caacaccatg aacgggttga tcgtgccctc gcccacgtcg ctcactagcg
     2581 cgttcgagat cttgtcctgc acgtagcgcg ggctgatgcc ttccatgcct tcgcgcttgg
     2641 tctccttgcg cagttccttc acggtgtcct gcgtgtagcc gggaagcacc ttgccgtcgt
     2701 agagcttcat cttctggagc agcgccaagt tgtgtttctt gggctcctcc aagcgcgtga
     2761 gcacggccca catcgaggcg acctcgagcg tgtgcggagc gacgtgcttg ccgcgcacct
     2821 tctcttggtt gaaatccttc tcgtaaatcc ggatctcgtc cgtcatccgc gtgatgtacg
     2881 ggatgtcgat cttgatcgtg cggtcgcgca aggcctccat gaactcgttg ttcagcaggc
     2941 gcttgtactc ggcctcgttg gtgtgcccga tgatcagctc gtcgatgtcc gtctgcgcga
     3001 acttcttggg cttgatgcgg tgctcttgcg aagccccgag caagtcgtac aagaacgcga
     3061 cgtcgagctt caaaacctcc acgaactcca cgatgccgcg gttcgcgata ttgaactcac
     3121 cgtcgaagtt gaaggcgcgc gggtcggagt ccgaaccgta ctccgcgatc tttcggtagt
     3181 tgatgtcacc cgtcagctcc gtcgagtcct ggttcttctc gtctttcggc tggaaggtac
     3241 caatgccgac gcggtccttc tcactgagca gcaggcgctt gacccgcacg tggttggaca
     3301 cgaccttgcc ccagtcaccc tggtagcgct ggagcaagcc gttgaagatg aaacggctcg
     3361 ccggatccat atcgccttcg atacgaacgc ggaacttgtc gttcgagagc ccgagcttat
     3421 cgatcgctcg ctcgcgccac tcctgaggga tcatgcgcag cggctcttcg tgcatgggcg
     3481 agtcgaacgt atcggcgccg ccgctcgcga gcccgctctc cccgaggttc acccacttga
     3541 aggtgaagag cgcgccctcg gtcgtctggg agtaacgctc gaggcccttc ttcaacagcc
     3601 gtgcgatcgt agatttcgaa gaaccgacgg ggccgtgcag caggatgacg cgcttctctg
     3661 gcccatagcc ttcggcggcg gccttgagca cgttcatcag gcgcatcagg ggaatgtcga
     3721 gaccgaagac ggcatccttt ccctcctcga tttcgtctcg gaagaagttg tagcgaacca
     3781 gcttcttctt attgtcgatg tactcctcgg ttccgtacga aatcaccatg tcgtacacgc
     3841 gctggaacgc attgcgtgtc acctgagggc gctctcgaac gatctggagg tactcttcga
     3901 agctgccttc ccaagccaga tcgcgatagc gatcgaagtc ctgcatggac gcaaccaacg
     3961 agaccatgct gcccgctgcg ggctcgttcg tgcgactgat cgtacccgat tgatccatat
     4021 tgtattccgc ctcgattcac gtcgaagtaa accacactga ccggccctcg tcaccattac
     4081 ctgcgaagcc accgaggaaa agggccgaag atgctcggac agcgagcgga gacgcgccgg
     4141 caacagtcgg ttcgcggccg gagccgtcag cggccgcgaa agagacgggt cggcactacg
     4201 agtgctcggg accgtggtcc cgagatgact ggtcggcact ggggcgtaga ttcaggccga
     4261 gcgcggccga cgcacgagcc gcaccgaatc cggcccgtgt gattcggcta gcaagcgcgc
     4321 gccatatcac cctcgtgagt atgcgtccgc caagggagcg cgttggtgaa gtacgcgagc
     4381 gtgcggacga agacccgcct tccgatgggt catgcaactc cgccgtgctc ggcaatcgaa
     4441 gtcgtcgacg cgactttcga cttacccggt cgagcccgac cgctccatgg cctgcgctct
     4501 acgtgaggcg tcagctcgtg caccgtaaaa caccgtgcag gtgagcggct ctctcggaaa
     4561 gagaggtccc tgacttgtga cgatcggatg acacgactta tgcttgaaga atctctccgc
     4621 atcctcgggc tatgctgaca cccgaacata catgtctgca cctgagcctc ttcgcgcctc
     4681 gctcaaccta cctagcgaga ttcccgttgc ggtcgtcccc ggccgactcg acgatccatc
     4741 gttgtatttc aatcgagaac tctcctggct cgagttcaac gcgcgtgtgc tcgcggaggc
     4801 gaacgacccg caagttccgc tctacgagcg cctgaagttc ctggcgatct tcgccagcaa
     4861 cctggacgag ttcttcatgg ttcgcgtggc cggcctgaag gcacagctct acggcgccgt
     4921 cgaggaggtc tcgcccgacg ggatgacccc cgagcagcag ctcgtggccg tggtcgatcg
     4981 cgtgcacgag ctgatggcgg agcagtaccg catctacacc tcggccttgc gccccgagct
     5041 cgcgcaagcg ggggtggcgc tcattcagcc cgaagaattg agcaccaagg aacaggccgc
     5101 gctcgatcag cacttcatcc aaaacatcta tccggtcctc acgccaatcg cgatcgaccc
     5161 ggtccatccc ttcccgcaca tccgcaacaa ggccatcaac gtcggcgtca tcttcgagaa
     5221 gcgcctgcaa tcgagcgagc cctcgttcgg cttggtgcaa gtgccaacga tgttgccgcg
     5281 cttgatccgc gtgccccgcg agggcttgcg gcgggcgttc gtcgcgctcg aggacgtgat
     5341 cctgcggcac accaaaacga tcttcccgca gctcaaggtg attggtcagt atgccttccg
     5401 cgtaacccgc aacttcgaca tcgagatcga cgaggaagaa ggcgaagatc tgctgattac
     5461 cttgcaagcg gagctgcgcc ggcgtgaacg cgggcacgcc gtgcgcctgg aagtgagcgg
     5521 ccaggcgccg cgcgaatcgc tgcagtggtt gtgccgtgag ctcgagctcg aatcgcgaga
     5581 cgtgtaccag gccgggggtc ccctgttcat cggggatatg acggtgctga cgtcggggga
     5641 cgagcggcgc gacctccgcg atgaggcctt ttcaccgcaa tacgtgccgc ccctgcgtga
     5701 ctccgaggac ttcttcgaga cgatcaagga acgcgacgta ctgctgcatc atccgtacga
     5761 atcgttcgag gcggtgatcg atttcttgtg gcaagccgcg gaagatccgc gcgtcctcgc
     5821 catcaagcag acgttgtatc gaaccggcgg cgagtccccg ctggtacgcg cgctgcaacg
     5881 cgcggccgag ctcggtaagc acgtgacggc gctggtggag ctcaaagcgc gcttcgacga
     5941 ggagagcaac attcaatggg cgagagcgct ggagcgctct ggcgtcaacg tgatgtacgg
     6001 cctactcggc ttcaagacgc acgccaaagt actaatggta gtgcgacgcg agcgagatcg
     6061 gttgcgccgt tacgtgcact tggccaccgg caactataac cagcaaacgg cgcggctcta
     6121 cgaagacctc tccctgttta cggcgcggga ggagtacggc gaagacgcga ccgccttctt
     6181 caacatgctg acgggctaca gcgcaccggt gcgctggaat cgctggatcg tcgcgccgct
     6241 cggcttgcac gaagcggtgc tcgggctgat cgcccgcgag accgagcatg ccttgaatgg
     6301 ccgccccgcc cgcatcgtcg ccaagatgaa ctcgctggtg gatgccagcg tgatcgccgc
     6361 gctgtacgcc gcttcccaag cgggcgtcga gatcgatctc ctggtgcgag gcatttgttg
     6421 cttgcgcccc ggcttacctg gcgtgagtga gcgaatccgg gttcaggccg tgattgatcg
     6481 cttcctcgag cacgcgcgaa tcttctactt ccagaacggc ggaaacgcgg aggtctattg
     6541 ctccagcgcg gactggatgc cgcggaattt ccggcgacgt gtcgaagtaa tgtttccggt
     6601 cgtggacgac gcgttgaagt cccgagtcat cgaggagatc ttgggagcga tgcgcgggga
     6661 ccgcgcgaag acgtggttgc tcggcgagag cggtgcgtac gtccgcatgg gtgacgctac
     6721 gggcctgcgc agccaacaac gtttcatcga tttggcgcgt gaacgcgcac gcgagaacga
     6781 ggccatcttg ggactgtcga agcccgtcgc gacgccaggt gggcgcgcac tagacaagtt
     6841 gcgacgtcga ggcggcaaga aacgcaagaa acgaaaccgc ggcgaaaacg acgactgatg
     6901 cgccgctcac aaaatggttg cggtcgagtg acggttttgc ccgtcgactc tcgtcagctc
     6961 gcgaaccctg tgacgcagcg gtggtgtgcg gatttcgcac ttcggcggat agtgagaatg
     7021 caatcgttcg ctgtagcaag ccacgtgggt ttggcctagt ctgcggattg gagatggcac
     7081 agcacgtgcc tcgtagggga tccgatcatg acttcaactt ctgccctcaa agcgcgtgcc
     7141 gcgctgggga atcgtcgttc gcatgggcgg gcaaaactgt gcgtcccagt gttgctggac
     7201 actgctcgga gttacttcaa agccttcagt aaggacgtgt cagagagcgg gatcggcatc
     7261 gaggtcgggg gacctgtcgg tgcaggcaac gagctcgaag tgggccgctg cgtagagctg
     7321 tacttcgagc ttccaacggg ctcgggcttg gagctgagcg ctgaagtcgt tcgtagcgag
     7381 gacgacggga ttgggctcac gtttcgcggg ctctcgccag aggcgagcca ggcgctgaag
     7441 cattactgcg aacagtggaa agcggagttg attcagcgct gccgaacgcg cgcctccaat
     7501 gactcctccg agcccgcaaa cgcatccgcc gcttcggttg cgcgttcgtt ctcggcgcgg
     7561 ccgatacgag cggcgcagcc tgccttctat gacgactcgg agatgtcgag cgagatccgg
     7621 atccgctacc gctagtccgt cgacgaaaga ctcgcgaaac gtacaggcgc gtctactcgg
     7681 acacaccctc gtggatgtgg cgccacacgg accagtctcc gcggcgttgg tgcagagcaa
     7741 aaccgggcaa tacggcagga ccgcctcgca ccacggccca gtcatagaag tcgagatccc
     7801 gctgcgtatc gacccattgg ggcaaccact cccagcgctc ggggaccggc ggggggcggt
     7861 gatgctcgcg aaacctaaag ggtgagctcg gaaactcggc gaacgtgaac atcacgattc
     7921 cgccacgttc cgcctggtac cacgccacgg cgtgaagata aggcgcgaac cgcacgtagg
     7981 cggagcgagc accgtaaatg aggcctacca cgtggctacc ctcggggata gcctcgattg
     8041 cggagccgaa gccggcgtat tcagtgcgtt cgatctctcg cagcaagcgc gcgaggttcc
     8101 aggacgtgag taaggttacc gccgtcagag tcgcgcctac caaccatcgt aaacgccgcg
     8161 aaatgctcgg caggacggcg acgcccagca acatggcgag tagcgggaag cgcgcgtgga
     8221 tcggccagat gaaggcgtaa gcagcgggca gcgagaagta ggcgaaccac gcgacgaaga
     8281 tc
//
LOCUS       DASTKU010000419         8626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_969988, whole genome shotgun sequence.
ACCESSION   DASTKU010000419 DASTKU010000000
VERSION     DASTKU010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8626
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969988"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VFQ61_14390"
     CDS             complement(<1..941)
                     /locus_tag="VFQ61_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955699.1"
                     /translation="MTHFSRGSSLVLDRARRFVPTSRIGRATATISLVAAFACSSKAP
                     SASGTQIDSAGSTNAPAGGTASGGANGTSIAGALVASGGRPTLPVAGSGNTPSTGTGS
                     SDGGSTSPPKEVTQECASSQTSSKDVTSVQPADIIFAIDTSTSMTAEIEFVQSYMNEF
                     SQMILNSGIDVRVILIAGPRDAVVGGGVGGAAGMGGAGGAAGMAGAAGAGGRRPAGGG
                     VVGGTLTQTVCIGPPLGSGQCPDDTKLPNYFHVQQYVDSHDALDVIVNAFPKYKEYLR
                     PNASKSFVVVSDDNAESTAAAFSSALTALDPVLFQKW"
     gene            2498..3190
                     /locus_tag="VFQ61_14395"
     CDS             2498..3190
                     /locus_tag="VFQ61_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HET9955700.1"
                     /translation="MGVNPRLPAKKDVALALLEQSTLYVHLDPRNQEVRVPPWFKKQP
                     QLVLQVGLNMAIPIPDLNVSDEGISCTLSFNRSPHFCWIPWGSVFALVGENGRAMVWP
                     DDVPKEVAAQSAQQQQKKEGPRLHSVPAEEPKAAQPSRPPAKPAAAATASEDSEKLQK
                     KKPRRAKKETGISVTDGSRTPSKPPVSERPRPAPALSASEPPAVAPAPRDSAPADSKR
                     KRDLPPYLRVIK"
     gene            complement(3274..3648)
                     /locus_tag="VFQ61_14400"
     CDS             complement(3274..3648)
                     /locus_tag="VFQ61_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955701.1"
                     /translation="MATACPAEEGESSCRKDADCPANYLCDSLVELCRPIEALSCHSP
                     ADCSDATCSRAGYCRAGSCDDPEVGCVEGYTCLARDGASVCEREGLGEGGAGGAGGTT
                     SSTSLEGSAGSIGQTSGEGGAN"
     gene            3993..6050
                     /locus_tag="VFQ61_14405"
     CDS             3993..6050
                     /locus_tag="VFQ61_14405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET9955702.1"
                     /translation="MDVRCNRCGTEYEFDDALISERGTTVKCTHCGHQFKVFGSRSEK
                     GLPERWTVRTSDGREIVYSDLRELQSDILERKLSALDRLSRGNQAARQLGAIAELAPF
                     FRGERSRKGRDSVEPPPPSEPDASTSPRAATPSAEPAPPLPEQEQTPHSGARSEQKPA
                     PASSRATGDAAAAAPTLPTSDDSTPAEKPAAPDVESERSALTGAAAEAAENSGAESSR
                     KPQGPQSPERTRDPSAANSPAALGAVSPVKSSPFTAHTPPASSPFSSSSASSSPFSSP
                     ISSTAHRSSPSNPRHHSYEELSAPPPSVGRRNLRSADEITGGPPSVGRRQLRSGNDLS
                     AAPPPVSRRKSYEELSASPPSVNRRNLRSYDELSGTPPSVSRRSLRSYENLMGLASVG
                     RHARSRWIAALVIAALAALFMLTVGRRYLPESVTAGRVGDGLRSHRVAELLREGQRLL
                     EDGDFEGASEPLLRASALSEKDPAVLAALARVATQRADVIWLKLRLLDPKLTEVVQAT
                     RKELGRRVGKARTAVDAAFVAAPEDIVVIRARVDALRLSGEATEAREWIRPIAANASD
                     PQNAYVLAALDLADASPSWASAIDRLRTAANGERTPGRAQAALIFALARSGRIADAEA
                     ELGRLDSGTTGGLLIDELRAFVKRSASPAGVPTETQSGKLNTAPPAGSGSPKAVQARG
                     EQP"
     gene            6047..7888
                     /gene="sppA"
                     /locus_tag="VFQ61_14410"
     CDS             6047..7888
                     /gene="sppA"
                     /locus_tag="VFQ61_14410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET9955703.1"
                     /translation="MNVSKLGPTHQWIKTWGLRRSALSLGVAALALAACSGRPKSTKP
                     SSEPAENERRILEFDLGAGAPEGASGGLFQTPAKRTYTGLVRALERGVESPLTAGVFL
                     HFGNQGVDFSRSEEIAELLGRYRKRNVPVVCHAHDLSNATSWLTLAGCNKVYVSAAGS
                     LETVGIAAELVHLKGLLDRLKIEVDFLSIGKYKSGAEPLLREEPSEQAKEALSSTLGS
                     MREAWLAGVELGRKGSAEKLELGPYSPEEARQLGLVDAIGYESDARAEIKRLAKTDRI
                     AHAFGPTRPEDSKGGIGELIRLLAQGGDDARGKPHIAVVPAEGAITIESSGPLESSGI
                     TSRALTKTLRRLREDSSVKAVVLRIDSPGGSPLASDLIWHELMELRKAKPIIASVGSM
                     AASGGYYIASGAHSIIADRTSIVGSIGVFGGKIVIGPALRQVGINSVAVPASPDPKAA
                     ARASYLSPFTPWDDATRERVRAHMQGIYDLFVARVAEARKMSLEAVRASAEGRIWSGT
                     QGKDRGLVDELGGLTRALELARKRAGLAGDVPVTIEGSREGLLEALLVGDDASEAEVR
                     AALVRYEQEHGWMREIPSEFTASVGTLSPLLTGETSVLALPFAVRVR"
     gene            complement(7938..>8626)
                     /locus_tag="VFQ61_14415"
     CDS             complement(7938..>8626)
                     /locus_tag="VFQ61_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955704.1"
                     /translation="PHSIKSGAWSPEAFLLNLAHFGFVFAIVRDAASLLGLALRVPAK
                     DTVRRVATEIAAADVPHSSPAAKKSSVLERVALRFDAQVAWALLGVLAAAVVNVALPD
                     ASLLPYVLTGTLGMAIFAASVPLHAAAAPPLALALMGKGLAWPAGFLLVWLAPLSAEL
                     DWKRRVPLAALGALSLELAARFWALPSPVMPTGFGTPAAALFLVLVAYRAYRVGLRGL
                     LATVAPHSPH"
BASE COUNT         1624 a         2782 c         2762 g         1458 t
ORIGIN      
        1 ctccacttct ggaataggac tggatccagc gcagttaggg cgctcgagaa cgccgccgcg
       61 gtcgactccg cgttgtcgtc actcacgacc acgaaggatt tgctcgcgtt cgggcgcaag
      121 tactccttgt acttggggaa cgcgttcacg atcacgtcca gcgcgtcatg gctatctacg
      181 tattgctgca cgtggaagta gttcggtagc ttggtgtcat ccgggcattg accggagccg
      241 agcggcggcc cgatacacac ggtttgcgtc agggtgccac cgaccacacc accaccggcg
      301 gggcggcgac cgccggctcc cgccgcgccc gccataccgg cggctccgcc cgcgccaccc
      361 ataccggcag cccccccaac gcccccaccc accacggcat cgcgcggccc ggcgatgagg
      421 atcacgcgca cgtcgatgcc gctgttgagg atcatctgag agaactcgtt catgtaggac
      481 tgaacgaact cgatctcggc cgtcatgctg gtcgaggtgt cgatcgcgaa aatgatgtct
      541 gcgggctgaa ccgaggtcac gtccttggac gacgtctgac tgctcgcgca ttcttgagtc
      601 acttctttgg gcggcgaagt ggagccgccg tcgctggaac cggtcccagt gctcggcgtg
      661 ttaccagacc ccgcgacggg caaggtgggc cgaccacccg aggcgacgag cgcgcccgcg
      721 atggaggtgc cgttcgcgcc accgctcgcc gttccgcccg cgggtgcgtt ggttgaacca
      781 gcggagtcga tctgcgtgcc cgacgcgctc ggcgctttgc tgctgcaggc aaaggctgca
      841 acgagactga tagtggctgt cgcccgaccg atgcgactcg ttggaacgaa ccgtcgcgct
      901 cggtccagga ccaaagaact cccgcgggaa aagtgggtca tcatcatatc cttgctaact
      961 gaggttcgat ggttggccgg ctcggtagcg aatctgtccg agaatggctc ggagcacttc
     1021 ggatctctcg cttgctctgc ctgtgctcgc ttttgcttcg ctcgtcggat ttcgcactca
     1081 cctactggtt ttgagatccg tcgcgcattt gagagatgtc gaagctggtt gatcagatga
     1141 gggctatcat cgtgaagtgt ccgaaccccg ctcgagcagg cggacagcgc aataatcgcg
     1201 aactgtgacg agaagccact tgcggctcga gttgaaggcc tcgagcacag tggccctctt
     1261 tcgagctaag tggctttttc gagcgaatga gctcatcgag cctgctggtt cgcgcgattc
     1321 ctgagttcaa acctatttcg tgtgcagacg aagcacattc gggtgatccc gatgcgatcc
     1381 tttcagccca ctcacctggc cgcccgtgct tcggaccatg ttctcgccga aatctacgac
     1441 cagaatgttg cggcttggcg cactcggaga aggtcgaacc aaactcccat gctgaacgag
     1501 tcgacggcgg ggtttgcccg agaacagggt ctgcccgaga acagggtctg cccgagaaca
     1561 gggttgcctt gagaaccaaa cccgcgatga cgggccttaa gtaccagcga ctcgattacg
     1621 acgcgcgttc acgagcagga cacacgggag gaagcaagag cgcgcaagca ggaagtggag
     1681 cgaacccgcg aattcagctc acgcgaggag taaggtacta catcgatcag gtgagttcac
     1741 gcgcaacttg caatacacga cacttcgggc ccacattggc actcaccctc ccgagtagcc
     1801 tttcggcctc tcgtcgtgcg acgagagcgg attaatgcac tcacggacga gcctcgtgac
     1861 gatcccgctg tctgatccgg tggcagcccg aatcgggcct tccccgacga ctcatccggc
     1921 cccgcgctcg tcgggaatcg cggtagtccg actccaatca tcatggttgg tgcacaccac
     1981 tcggctgcac cgactcgtgc cttgattggc ttggtcggcg agatcgcgcg tagtccttct
     2041 ccgcggcgcg cggtgtaggc gccctgatct agcgtaaggc ccaccgcgag ctcgcaaagt
     2101 gattcgaaca gcgcagaatg cgccatccga aagcaatttt gcgaagtccc gttacctcga
     2161 cctccatggg gcgcgacgga gttcgactgg tcgaaacggc tcgtcgaaac ggctctgagt
     2221 ctcgcgcgat cgcatcatgc attcagcacg ccgtttggag tccccgctgt tccgctgtcg
     2281 atctctacgg cgcggctacc ccaaccggga aggaagagcc gagaatcgcg acgcagagcg
     2341 gcccttagcc cgcccgctct agccctctcg ccacatggcc ttgttcatcc gagcagtcgt
     2401 cccagcacga ggcagcaccc gcggagtcgc aaaggtgagg cgtcctcggc tatggcgctg
     2461 gtgtttcgtt cgcagcggag ctatgtgggt gtaccatatg ggcgtgaatc ccaggctgcc
     2521 ggccaaaaag gacgtggcgc ttgctctcct cgagcagtcg acactttatg tgcatctgga
     2581 cccgcgcaat caagaagtgc gtgtcccgcc atggttcaag aagcagccgc agcttgtcct
     2641 gcaagtgggg ctcaacatgg ccataccaat ccctgacttg aacgtcagtg atgagggtat
     2701 ttcctgcacg ctgagcttca atcgttctcc gcacttttgc tggattccat ggggttctgt
     2761 gttcgcgctg gtgggtgaaa acgggcgagc catggtgtgg ccggacgacg tgcccaagga
     2821 agtcgcggcc cagtcggccc agcagcaaca aaagaaggag ggccctcgtc tgcactctgt
     2881 tcccgcagag gaaccgaaag cggcgcagcc ctcacgtccg ccagccaagc ccgcggcggc
     2941 tgccacggcc agtgaagact ccgagaagct tcagaagaag aagccacgcc gagccaaaaa
     3001 ggagaccggg atctcggtga ccgacgggtc gcgaacgccg agcaagcctc cagtttctga
     3061 gcggccccgt ccagctccgg cgctcagtgc gtccgagccg cccgccgttg cgcccgcgcc
     3121 gcgcgatagc gccccggcag attcgaagcg gaagcgcgat ttgccgccct atctgcgtgt
     3181 gatcaagtag agggcaaagc cctcttaggg ctcactcaac gagctttttt gctggtttca
     3241 tcgaacggcg cgctccggtg ctcgcggcgg ccttcaattt gcgccgccct ctccggaggt
     3301 ctgcccgata ctaccggcgc ttccttccaa cgacgtgctc gaagtagtgc cgcctgcgcc
     3361 gcctgcgccc ccttcaccca ggccctcccg ctcgcaaacg gacgctccgt ctcgagcgag
     3421 acacgtatag ccctcgacgc accctacttc cggatcgtca cagctgcccg cccgacagta
     3481 gcccgctctc gagcaggtcg cgtcagagca gtcagccggt gagtggcacg aaagtgcctc
     3541 gattgggcgg cacagttcca cgagcgaatc gcacaagtag ttcgcggggc aatccgcgtc
     3601 cttgcgacag ctgctttcac cctcttctgc ggggcaggcc gtagccatca gccctgctac
     3661 aagtgacgcg acagcccaat tcaagcgttt catcgtaagc acgtgcctct aaggtcgaag
     3721 cgggtcccct gaagagaggg gccccaagaa cggtcccaaa gcggaggcca agggtaccgt
     3781 attggtctaa ggaaagtatc ggcttacgaa agcccccgcg gcgggccccg ctcccaagca
     3841 gagcgggccg aagtgatcgg gctcagaaaa tcccgaaccc agatcgggac atgctagcgt
     3901 cccgaggtct gatgcggcac cctgaaacca cggcctttcg acggctcttc ccctacggag
     3961 agggcgctcc catcgcttga tggggttatc cgatggacgt tcgctgcaac cgctgtggaa
     4021 ccgagtacga gttcgacgat gcgttgatct cggagcgcgg gacgacggtc aagtgcacgc
     4081 attgcggtca ccaattcaag gtcttcggtt cgcgctcgga aaaagggctg ccggaacgct
     4141 ggacggtgcg aacgagcgac ggccgcgaga tcgtctatag cgatttgcgg gagcttcaga
     4201 gcgacatctt ggagcgcaag ctcagcgcac tggatcgctt gtcgcgaggg aatcaagccg
     4261 cgcgtcagct gggcgcaatt gcggagctag ccccgttttt tcgtggcgag cgctcgcgca
     4321 aggggcgcga tagcgtagag ccccctcctc cttcggagcc ggatgcttcg acgtctcctc
     4381 gtgcagcgac accctcggcc gagcccgcac cgccgctgcc cgagcaagag cagacacccc
     4441 actccggcgc gcgcagtgag caaaaacccg caccagcatc cagccgagca acgggggatg
     4501 ccgcagcggc cgcgccgaca ctacctacct cggacgactc gacgcctgcg gagaaacccg
     4561 cggccccgga cgttgaatcg gaacgctcgg cactcacggg agctgcagcc gaagctgccg
     4621 aaaacagcgg agctgaaagc agccgtaagc cacagggccc gcagagtccc gaaaggacgc
     4681 gcgacccgag tgccgcgaac tcgccggctg cgcttggcgc ggttagcccg gtcaagagct
     4741 ctccctttac ggcccacacc cctcccgcga gttccccctt ttcgagctca tctgccagca
     4801 gttcgccttt cagctccccg atctcgagca cggctcaccg cagctcgccc tcgaatccga
     4861 gacatcactc gtatgaagag ctcagtgctc cgcccccgtc cgtaggtcgg cgcaacttgc
     4921 gctcggcgga tgaaataacg ggggggccac cctcggtggg gcgtcgccaa ttgcgctccg
     4981 ggaatgattt gagcgcggct cctccaccag taagtcgacg caaatcgtac gaggagcttt
     5041 cggcctctcc gccttcggtc aatcgtcgga atctacgctc gtacgatgag ctttccggca
     5101 cgcctccgtc ggtgagccgc aggagcttgc ggtcctacga aaacctgatg gggttggctt
     5161 ccgttgggcg gcatgcgcgt tcacgttgga tcgcagcgct cgtgatcgcg gccttggccg
     5221 cgctcttcat gctcaccgtg gggcgccgct acttgccaga gagcgtcacg gcggggcggg
     5281 tgggcgatgg cttgcgaagc caccgggtcg cggagttatt gagagaaggc cagcgcctgc
     5341 tcgaggacgg cgatttcgaa ggcgcgagtg agcctttact gcgcgcctcg gcgttatcgg
     5401 agaaagatcc cgcggtgctc gcggccttgg cacgcgtcgc cacccagcga gcagacgtga
     5461 tttggctcaa gctgcgcctg ctcgatccca agctcacgga ggtggtgcag gccacgcgca
     5521 aggagctggg tcgacgcgtt ggcaaagcgc gcacggcggt ggacgccgcc ttcgtcgccg
     5581 ctccagaaga catcgtggtg attcgagctc gcgtcgacgc gttgagactg agtggcgaag
     5641 ccaccgaagc gcgggagtgg atccgtccga tcgcggcgaa tgcctccgat ccgcaaaatg
     5701 cgtacgtgct ggcagcgctc gatctggcgg atgcttcgcc gtcctgggcc agcgctatcg
     5761 atcgtttgag aacggccgcc aacggcgagc gcacgccggg gcgcgcgcag gcggcgctga
     5821 ttttcgcgtt agcccgctca ggtcgaatcg cagacgccga agcggagctg ggccgcttgg
     5881 actccgggac gaccggggga cttctgatcg acgagctgcg cgcgttcgtg aagcgatcgg
     5941 ccagcccggc aggcgtgccg accgagacgc aatccgggaa actcaatact gcccccccag
     6001 caggctcggg ctcgcccaag gcggtccagg cgcgcggcga acaaccatga atgtgagcaa
     6061 gttgggccca actcatcagt ggatcaaaac gtggggatta cgccgatcgg ccctcagcct
     6121 cggcgtcgcc gcgctggcgc tcgcggcttg ctcgggccgc ccgaagagca ccaaaccatc
     6181 cagcgaaccg gcggagaacg agcggcggat tctcgagttc gacctcggcg ccggcgcccc
     6241 ggagggcgca agcgggggtt tgttccagac gccggccaag cgcacgtaca cgggtttggt
     6301 gcgcgccttg gagcgaggcg tggagagccc actcacggcg ggggtcttcc tgcactttgg
     6361 caaccaaggc gtggatttct cgcgttcgga agagatcgcc gagttgctcg gacgttatcg
     6421 gaaacgcaac gtgccggtag tgtgccacgc gcacgatctg agcaacgcaa cgagctggct
     6481 cacgctggcg ggttgcaaca aggtctacgt cagcgcggcg ggttcgctgg aaacggtggg
     6541 catcgcggcc gagctcgtgc acctcaaagg cctgctggat cggctcaaga tcgaagtgga
     6601 ttttctatcc atcggtaagt acaagagcgg cgccgagccg ctgctgcgcg aagagccgag
     6661 cgaacaggcc aaagaggcgc tctcgagcac gctcggttcg atgcgtgaag cgtggctcgc
     6721 cggcgtcgag ctgggacgta aaggcagtgc cgaaaagctg gagctcggtc cctatagccc
     6781 ggaagaagcc aggcagctgg gcctggtcga cgcgattggc tacgagtcgg acgcgcgcgc
     6841 cgaaatcaag cgactcgcaa agacggaccg catcgcacac gccttcggcc caacccgacc
     6901 cgaggactcc aaaggtggaa ttggcgagtt gattcggctg ctcgcgcaag gcggcgacga
     6961 tgcacgaggt aagccgcaca tcgcagtagt gcccgccgaa ggcgcgatca ccatcgagtc
     7021 gtcggggccg ctcgagtcct cgggcatcac ctcgcgagcg ctcaccaaga cgttgcgtcg
     7081 gctgcgcgag gacagctcgg tcaaggcggt cgtgttgcgg atcgactctc ccgggggctc
     7141 cccgctggcg agcgatctga tttggcacga gctgatggag ttgcgcaaag ccaagccaat
     7201 catcgcttcg gtcggcagca tggccgcgag tggcggttac tatatcgctt cgggtgccca
     7261 cagcatcatc gcggaccgta cgagcatcgt cggctcgatt ggcgtgttcg gaggcaagat
     7321 cgtgattggc cccgcgctcc ggcaggtggg gatcaactcg gtggcggtgc cggcgagccc
     7381 cgatcccaag gcggccgcac gtgcctccta tttgtccccg tttacgcctt gggacgacgc
     7441 gacgcgcgag cgggtgcgcg ctcatatgca gggcatctat gatctgttcg tagcgcgcgt
     7501 tgcggaagcc aggaagatga gcctcgaggc cgtgcgcgcc agcgccgaag gtcgcatttg
     7561 gagcggtacg cagggcaagg accgcggtct cgtggacgag ctcggcggtc tcacgcgagc
     7621 gctcgagctc gcccgcaaac gtgcaggcct cgccggcgac gtgccggtca cgatcgaggg
     7681 ctcgcgcgaa ggcttactgg aagcgctcct ggtcggtgac gacgcgagcg aggccgaggt
     7741 tcgagcggcc ctcgtgcgtt acgaacaaga gcacggctgg atgcgcgaga ttccgagcga
     7801 gttcacggcc agcgtcggca cgctcagccc cttactcaca ggggagacct cggtcctggc
     7861 cttgcctttc gcggtacgcg tgcgttgagc gcgccggagc gtgaagaccg cgggctcgcg
     7921 ctcctagcca gcacgattca atgcggagag tgcggcgcaa ccgtcgcgag cagcccacgc
     7981 aaacccacgc ggtacgcgcg ataagccact agcaccaaga acaaagcggc cgcaggcgtg
     8041 ccgaaccccg tcggcatcac tgggctcggc aaggcccaga accgcgccgc gagctcgagg
     8101 ctcaacgctc caagcgccgc caacggcacg cgacgtttcc aatccagctc ggcgctcagg
     8161 ggcgccaacc acacgagcaa gaatcccgcg ggccaagcga gccctttgcc catcagcgcc
     8221 aacgcgagcg ggggtgccgc cgcggcgtgg agcggcacag aagccgcaaa gatcgccatc
     8281 ccgagcgtcc cggtgagtac gtagggcaag agcgaggcat ccggcaaagc aacgtttact
     8341 acggcggccg cgagcacgcc cagcagagcc cacgcaactt gagcgtcgaa gcgcagcgcc
     8401 acgcgctcga gcaccgacga cttctttgcg gcgggcgaag aatgcggcac atccgcggcc
     8461 gcgatctcgg tcgccactcg ccgcaccgtg tctttcgcgg gcacgcgcaa tgcgaggccc
     8521 agcaacgagg ctgcatcgcg cacgatcgca aacacgaagc cgaagtgggc gagattcaac
     8581 aagaaggctt ccgggctcca ggcaccactc ttgatcgagt gaggtg
//
LOCUS       DASTKU010000420         3544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1640286, whole genome shotgun sequence.
ACCESSION   DASTKU010000420 DASTKU010000000
VERSION     DASTKU010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640286"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VFQ61_14420"
     CDS             complement(<1..767)
                     /locus_tag="VFQ61_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955705.1"
                     /translation="MEGPILSHTRRIADQLAQEFRGNPERELEIWATTAQQREAMVVE
                     LYDAAAIENRFPKRAGEPEVSSVVRKAIGGIWAQERSHTTLVESLRIVDEARLTALRS
                     MLGAMEGRVTHTATAPSWTRSIASFLVGLGRTAGHAPEFTSGFRSLSLREFFRFSQEL
                     ETTAKEGYERIVELLDRLVPNDALAAIDSRQKAGAIKLGITAPYEFAKTLAEECFHAA
                     VFERLEAWLEPDGHSFEPLSAHDAVLQLRALAVEHLS"
     gene            complement(947..1954)
                     /locus_tag="VFQ61_14425"
     CDS             complement(947..1954)
                     /locus_tag="VFQ61_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9955706.1"
                     /translation="MRSNHLDVLRVVEAAYELDSTNEEWLKSIAEAVRPHLDDGFGLA
                     AFEFMRVGDEVPTVLQSQHLWMPESLAQVYPRMFASMPPELRLRPFRLGPCITGSEMM
                     NMKEDFANLPQMKNGLQKFGMFDSIWITATDPTGRGCGFHAGRKAIAWAGPAEKQTWG
                     QIAAHLASAIRLRYRLRQAEAREPLAAVFEPNGKLHDAVGEAQETEARALLRDAVLRI
                     ERARGQQRHEAPEQALREWKALVAGKWSLVDRVEHDGRRYIVARENEPRAPGPAALSE
                     RERQILGYAQLGHHNKLIAYELGIAQSTVRVLMSRAMSKLGVRTREELLETIRQVPLQ
                     P"
     gene            2208..2936
                     /locus_tag="VFQ61_14430"
     CDS             2208..2936
                     /locus_tag="VFQ61_14430"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9955707.1"
                     /translation="MTAQERQWIDTLTQRVEAASRVETAQHVDSGPASVERCSALWIA
                     LVSGGLQIVQALAQEQQSVLILKARAVPVPPLGPRTHQILERTLLGESRKVIAYETNV
                     SPSTVAFALKEALAGLGLRCRPSMVPSSLVILAHAACRGIPPTSSFISDGEHQDQHFT
                     VFTHVFEDAVLRGLSPSERVVVRLLASGRSCGDIAARRNRSCRTVVNQVAAACRRLGV
                     SGRLELLHRFATLAVSEPPQATNR"
     gene            3240..>3544
                     /locus_tag="VFQ61_14435"
     CDS             3240..>3544
                     /locus_tag="VFQ61_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955708.1"
                     /translation="MRTSLSERIDQLALVWLVVPRKESRLNGFVSQLKQLATGAEDRK
                     ELARDSIERLRKNRLVEEGALLKLTDEGRRRALEVLGVAKLPQGRGTLQWAKKVLLLR
                     "
BASE COUNT          689 a         1093 c         1079 g          683 t
ORIGIN      
        1 aatgagagat gttcgacggc gagcgcgcgg agttggagaa ccgcgtcatg agccgagaga
       61 ggttcgaagc tgtgaccgtc aggctctaac caggcttcta gccgttcgaa cacggccgcg
      121 tggaagcact cttcggcgag ggtcttggcg aactcgtaag gcgccgtgat gcctagtttg
      181 atggcaccgg ccttttggcg cgaatcgatg gcggcgaggg catcgttggg aactagacgg
      241 tcgagaagct cgacgatgcg ctcgtagcct tcctttgccg tagtctcgag ctcctgagaa
      301 aagcggaaga attcacggag gctcagcgag cgaaagccgc tcgtgaattc gggggcgtga
      361 cccgcagtcc gaccgagccc aaccaggaag ctggcgatgg atcgcgtcca gctgggggcg
      421 gtggcggtgt gagtgactcg accttccatc gcgccgagca tcgaacgcaa cgccgtaagg
      481 cgtgcctcat cgacaatccg gagcgactcc accagggtgg tgtgggagcg ctcctgcgcc
      541 caaatgccgc cgatggcttt gcgaactacg ctcgagactt ccggttctcc cgcgcgcttg
      601 gggaagcgat tctcgatcgc ggcggcatcg tatagctcga ccaccatcgc ctcgcgctgc
      661 tgggcggtgg tcgcccaaat ctcgagttcc cgctcgggat tgccacgaaa ctcctgcgcg
      721 agctgatcag caatacgccg tgtatgcgag aggatcggtc cttccatgtt cgagtccctt
      781 cggccctaga agtacgacat tcgcgacgag gaccttatag taaaaaatca ggagactcgc
      841 cgtgactgca ccagccgctt gcgaaaccgg agcaggaatg acagcttgtc ggccgtagcg
      901 aggctcgatg agccgagatg acgtgagcat ctcggccgca cgagcgtcac ggttgaagcg
      961 gcacttgccg aatcgtttcg aggagctcct cgcgggttcg gaccccgagc ttggacatcg
     1021 ctcgggacat caagacgcga accgtggact gcgcgatacc gagttcgtat gcaatgagct
     1081 tgttgtggtg gccgagctga gcatagccaa gaatctgccg ttctcgttcg ctgagcgcgg
     1141 ccgggccagg cgcacgtggc tcgttttcgc gagcgacgat gtagcggcgg ccgtcgtgtt
     1201 cgacgcgatc gacgagagac cacttgccgg ccaccaatgc tttccactca cggagcgcct
     1261 gctcgggagc ctcatgtctt tgctggccac gcgctctctc aatccgcaaa acggcatccc
     1321 gaagcaaagc tcgggcctcg gtttcctgcg cttcaccgac cgcatcatgc agcttgccgt
     1381 tcggctcgaa gacagctgcg aggggctccc gtgcttcggc ttgacgcaac cgatagcgaa
     1441 gccgaatggc gcttgccagg tgcgcggcaa tctggcccca agtctgcttc tctgccggcc
     1501 ccgcccaggc gattgccttg cggcccgcgt gaaagccgca gccccgtccc gtcggatcgg
     1561 tcgccgtgat ccagatcgag tcgaacatcc cgaacttttg caggccgttc ttcatctgcg
     1621 gaagattcgc gaagtcctcc ttcatgttca tcatctcgct gcccgtgatg cagggcccga
     1681 gccggaatgg tcgcagacgg agctccgggg gcatgctcgc gaacatgcga ggataaacct
     1741 gggccaagct ctccggcatc cagaggtgct ggctctgaag aacggtcgga acttcgtccc
     1801 ctacccgcat gaactcgaag gcagcgaggc caaagccgtc gtctaagtgc gggcgaaccg
     1861 cttcggcgat gctcttgagc cactcctcgt tcgtggagtc gagctcgtaa gcagcctcca
     1921 ctacgcgcag cacgtccaag tgattggacc tcattcgtga tcggaagata tcccaacccc
     1981 gtgatgcgac cagaggcctg tgctggcgtg tgagtttatt tcaagatagc gcaacaaaac
     2041 caggcgctta cgccggtgga tgtaagctcc tctttcgttg aaccttcggc ttttcgggcg
     2101 cacgctgggc atcactcacg gggcggcaac gcgcgcgaaa ccgctgacac tggctgtgcc
     2161 gcgccgagcg cgtgcctggg ctccgtgcgc tcaccggtca ggcccacatg acagcccaag
     2221 aacgccaatg gattgacacc ctaacccagc gagtagaggc cgcgtcgcga gtggaaacgg
     2281 cgcagcacgt cgactctggc ccggcgtccg tcgagcgctg ctcggcgctt tggatcgcgt
     2341 tggtttcggg cgggctccag attgtccaag ccctagcgca ggagcaacag tccgtgctga
     2401 tcctcaaggc acgagcggta cccgttcctc ctctcggtcc acgcacccat caaatactcg
     2461 agcgcactct gttgggagag agccgcaagg tgatcgcata tgagacgaac gtctcgcctt
     2521 cgacggtcgc ttttgcattg aaggaagccc tcgcgggcct cgggttgcgt tgccgaccat
     2581 ccatggttcc gtcgtctctg gtgattctcg ctcacgctgc gtgtcgcggg atcccaccga
     2641 ctagtagctt tattagcgac ggcgaacacc aagaccagca cttcaccgtt ttcacacacg
     2701 tgttcgaaga cgccgttctg cgggggcttt ccccgagcga gcgtgtcgta gtgagattgc
     2761 ttgcgagcgg ccgcagctgc ggtgatatcg ccgcccggag aaaccgctct tgtcgcaccg
     2821 tagtcaatca ggtagccgcg gcctgccggc gactcggagt ctctggtcgg ctcgagctcc
     2881 tgcaccgttt tgcgacactg gcggtgagtg agcccccgca agccaccaat cgctaaacga
     2941 ccggcgcgca ccgttttctt catcatcgcg gcccccggct caacactcaa gctgatcccg
     3001 cggctccgtt cgccactcgc gagaagggct gttagcgcgg tgtcatcata ccgactcacc
     3061 agacacaccc atcagcacca cccttacata ctgattctca caatcggcat atccagaaaa
     3121 caatgactat cgtcgagaca ggcaaaaacc aatacggtct ttgcgtttga ggacgacgtc
     3181 ggcgcgcata cccgcccggc tcggggctcg actaccattt gcaaatacta gggaggaata
     3241 tgagaacgtc tctaagtgaa cgtatcgacc aactcgcttt ggtgtggctc gtggtgccac
     3301 gtaaagagag tcggctcaac ggcttcgttt cgcagctgaa gcagctggca actggagcgg
     3361 aggatcgcaa agaactggct cgcgacagta tcgagcggtt gcgcaagaac cgcctcgtcg
     3421 aagagggagc tcttctcaag ttgactgacg agggtcgccg tcgtgcgctc gaggtgcttg
     3481 gagtggcgaa gctcccgcaa ggccggggaa ccctacaatg ggccaagaaa gtgctcctac
     3541 tgcg
//
LOCUS       DASTKU010000421         7385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_79135,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000421 DASTKU010000000
VERSION     DASTKU010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79135"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFQ61_14440"
     CDS             complement(<1..449)
                     /locus_tag="VFQ61_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9955709.1"
                     /translation="MGQRILVVEDEPAIAESVAYALTRDGFAVEIAATAAAAAPLIAQ
                     SELVILDLMLPDDSGFNLISRARGAGVVTPIIVLSSRDDESDRVAALEAGADDYVTKP
                     FSPREVVARVRAVLRRVHGSSSESHEHEPRIRVDLSTRRAYVEQREL"
     gene            complement(465..986)
                     /locus_tag="VFQ61_14445"
     CDS             complement(465..986)
                     /locus_tag="VFQ61_14445"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9955710.1"
                     /translation="MRTLLVLRHGKAVRDTSELSDHARPLKKKGVREAEVVGQYLRAN
                     GLLPDHVLCSTAERARDTAQRALRAAELEPPIEYLNELYLAEPPAYVEALRRHAADAE
                     RALVVGHNPGLEALVYLLTGEERVLDTGALVVCELPIDAFGELPAAGRGTSVRELPSR
                     IGKVVGFFQPQED"
     gene            complement(1147..4125)
                     /locus_tag="VFQ61_14450"
     CDS             complement(1147..4125)
                     /locus_tag="VFQ61_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9955711.1"
                     /translation="MQKTIAVLIARVDHVDDGYEAELRRAFASACAARQLSLCIVVGR
                     ALGESPHDRVYRLIQRGSVDGVILVTSGLAAHGGLAAVERLLPHFEGLPLCSFGVRVP
                     GVPSVVIDNSAGMRSAVEHAVLSHSIQRPVFIGGPDHDQDASERAAACRAVLGEYGLE
                     LPAEYELSAELGAASAQVAMRAFLARGLPFDAVLAVNDNVAVGASAALKEAGLRLPQA
                     ACVIGFDDLPSSRFSNPRLTTVRQPLDAMAERAVDLVLTQALGKSGVSAGKDEICELP
                     LSLLCRASCGCQSSELETRRRPHSAHDPAVLLEAEAQDLLRQLSRAFRLPQRESRAAA
                     ELLLRELARELSGQAGAFIAALERLMSQYAEALEIYEELQGVINLLRARLRSERVCLE
                     DLWHTARGAISSASTAEQARRRWSSEAAHARALASADRLSTAFDWASLKAALSAELTK
                     VARNAFISLYASPDERELRPFFCLSEGRLVEPRATRFRASSLLPPGEPCGERCHLLYV
                     FPLTFEHESLGVMVFELLPGVGTREILREQIGAALKSVALHREIVNKTALHERSVQER
                     EATARRMASLSVLAGGVAHDLNNALGPLVALPDILLTELDRVRDGASDVELRQDIETI
                     RTSALRATQTIKDLLALGRQGRTAREPLDLNQTVTACLAAESQCIDRAAQRGVRVEPE
                     LEAESLWIRASDAQLVRAVSNLLRNAIEATEGPGLIRIRTSSVRLLEPLSGYETIEPG
                     DYARVVVSDPGKGIEPADLARVFEPFFTRKRLSESSGTGLGLAIVHGVVKEHEGFVNV
                     ESRLGVGTQFTLYFRLTPDRPRPLRRRRPSGPRGARILVVDDDPVQLRTAERVLSRLG
                     YQVSTSQSGKRSFQRFSDELGGHENESPYDLVIMDVLLNEEDDGLTVFERMQTVSPAQ
                     RGIVVSGHAPTERVKRAISQGLAWLEKPYGADELAQAVQSALERPAPNASSALGAGGL
                     GSAGALGTSGSTAGRARVAS"
     gene            4298..5416
                     /locus_tag="VFQ61_14455"
     CDS             4298..5416
                     /locus_tag="VFQ61_14455"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HET9955712.1"
                     /translation="MSHPERDLYAQAGVDYGTMDPGKRRAQLAALRTARTLSEKGLSE
                     VTESRGESAYVVDFGDHYVSSVTEALGTKNLVADAVRPITGRSHYEQIGKDTVATVLN
                     DLSSVGGSPVSLTAYFGAGDSAWFQDEARFNDLITGWESACLEAGCAWGGGETQVLVG
                     MISPETVVLGGSAVGCIRPKPQLLAGRRIQAGDAILLLEASGIHANGLSLARSIAKQL
                     PRGYATPVPGDPQGRGFGEVLLDATPLYGPAVSALQSADIELHYAVNVTGHGLRKLMR
                     ADAALSYIVERMPAPNPIFEFLREATGMSELEAYGTFNMGIGYALYLRADHAPRALQV
                     LEARGIKAMLGGHIESGPKRVVLEPNGLEFSGETLAIR"
     gene            5511..6113
                     /gene="purN"
                     /locus_tag="VFQ61_14460"
     CDS             5511..6113
                     /gene="purN"
                     /locus_tag="VFQ61_14460"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET9955713.1"
                     /translation="MLKLGMLASHRGSTVERLVQACRDGRVRGEPRVVISNNSGARVL
                     EVAKAAQIPAFCIGGAAYASEELRDRAILETLQRHGVELVLLLGYMRKLGPETLRAYR
                     GRVLNTHPALLPKFGGQGMFGMHVHRAVVAAGETESGATLHVVDEWYDHGPVLLQRRV
                     PVLTGDDPEALQQRVQGIEHELLVEGLNSIESGQLVLPAP"
     gene            6280..>7385
                     /locus_tag="VFQ61_14465"
     CDS             6280..>7385
                     /locus_tag="VFQ61_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HET9955714.1"
                     /translation="MKSDVTVVCGVNWGDEGKGRMVDYLAEDAQIVARFQGGNNAGHT
                     IINQYGHFALHLVPSGIFRPSVYNLLGPGMVIDLEGLVDEMARLNQRGVSTERLLLSD
                     RATVSFPFHRKLDEWEEKRLAGRAYGSTKNGIAPAYGDRHLKKAVRIGDLFDRDGRRD
                     AIARAVEWANLLARDVYHEDGFTDAQMLAWCDQFLPRLEPHVTDTVTFLENAVQEGKN
                     VLLEAQLGALRDVYFGIYPYTTSSCALSAFAPIGAGLFGVKANRVVGVMKAFATCVGE
                     GPFVTRMTDEEASELRESAKEYGARTGRPRTIGHFDAVASRFGAQVQDTTELALTKLD
                     SLSGHKELKLCTAYNVDGKQTERFPVTGLYRATPV"
BASE COUNT         1301 a         2445 c         2380 g         1259 t
ORIGIN      
        1 tcgagctcac gctgttcgac gtaggcccgg cgggtgctca aatcgacccg aattcgcggt
       61 tcgtgctcgt ggctttcact cgagctcccg tgcacgcgcc gtagcaccgc ccgcacgcgc
      121 gccacgactt cacggggcga aaagggcttg gttacgtagt cgtctgcacc ggcttctagt
      181 gccgccacgc gatcggactc gtcatctcgg ctggacaaca cgattatggg cgtcactacg
      241 cccgcgcccc gagcccgcga gattaggttg aagccgctat cgtccggcaa catcaaatcc
      301 agaatcacga gctcgctctg ggcgatcagc ggagcggccg cggctgccgt ggcggcgatc
      361 tcgaccgcga agccatcacg cgtcagcgcg tacgccacgg attcagcgat ggcgggctcg
      421 tcctcgacga ccagaatgcg ttgccccatt tgatcgctcg cgcttcagtc ttcctgcggc
      481 tggaaaaaac cgacgacttt accgattcgc gagggcagct cgcgaaccga cgtcccccgc
      541 cccgccgccg gtaactcccc aaaggcatcg atgggcagct cgcacacgac gagcgccccc
      601 gtatcgagta ctcgctcttc gccggtcagg agatagacga gcgcctcgag cccggggtta
      661 tgacccacga ccagagcgcg ttcggcgtcc gcggcgtggc ggcgcagggc ttcgacgtaa
      721 gccggaggtt ccgccaggta caattcgttt agatactcga tcggcggctc tagctccgcg
      781 gcgcgcagcg cccgctgcgc ggtgtcgcgc gcgcgttcgg cggtcgagca caggacgtga
      841 tcaggcaaga gcccgttcgc cctgagatac tgacccacca cctcggcttc acgcacgccc
      901 ttcttcttca gcgggcgagc gtggtccgag agctcggacg tatcccggac agccttaccg
      961 tgtctcagca ccagcagtgt tctcatggcc gaaggttccg gggctagact agcgcgtccg
     1021 actgcagcgg gaccactgcc accccaaatc gccattgacc tagaatcgcc attgacctga
     1081 aattgccggg ccggggaacc gtgccgcgca acgtattccc cgcgcgagca acgccgggtg
     1141 ttcgattcag gaggcaaccc gagcgcggcc ggccgtgctt cccgaggtgc cgagcgcgcc
     1201 agctgacccg agcccgccgg caccgagcgc gctcgatgcg ttgggcgctg ggcgttccag
     1261 cgcactctgc acggcctgcg caagttcgtc ggcgccatac ggcttttcta gccaggcgag
     1321 gccctgcgag atggcacgct tcactcgctc ggtgggcgca tgcccgctca ccacgatccc
     1381 gcgctgcgcg ggcgaaaccg tctgcatgcg ctcgaagacg gtcaggccgt cgtcttcctc
     1441 gttcagaagt acgtccatga tcacgaggtc gtacgggctc tcgttttcat gcccgcccag
     1501 ctcgtcgctg aagcgctgaa agctacgttt accgctttgg ctggtggaga cttggtatcc
     1561 gagccgtgac aatacccgct cggccgtgcg cagctgcacg gggtcatcgt ccacgaccag
     1621 gatgcgtgcg ccgcgtggac cgctcgggcg acgccgacgg agcggccgtg ggcgatcggg
     1681 ggtaagcctg aagtacaacg taaattgagt tccaaccccc aagcggctct ccacgttgac
     1741 gaagccctca tgctctttca ccacgccgtg cacgatggcg agcccgagcc cggtgccgct
     1801 gctctcggac agccgcttgc gcgtgaagaa tggttcgaag acgcgggcca agtcagcggg
     1861 ctcgatgcct ttacccgggt cgctcacgac caccctcgcg tagtccccgg gctcgatggt
     1921 ctcgtagcca gacaacggct ccagcaagcg cacgctggag gtccggattc gaatcaggcc
     1981 ggggccctcg gtcgcttcga tggcattgcg cagaaggttc gacacggctc ggaccagctg
     2041 ggcgtccgaa gcccgaatcc acaagctctc ggcctcgagc tcgggttcta cccgcactcc
     2101 gcgctgcgct gcgcgatcga tgcactgcga ctcggccgca aggcaagcgg tcacggtttg
     2161 gttcaagtcg aggggttcgc gggcggtgcg cccttggcgc ccgagcgcca acagatcctt
     2221 gatggtttgg gtcgcgcgta gcgccgacgt gcgaatcgtc tcgatatctt gccgcagctc
     2281 cacatcgctc gcgccgtcgc gcacgcggtc gagctcggtg agcaggatgt cgggcagcgc
     2341 gaccaacggc ccgagcgcgt tattcaagtc gtgggccacg ccgcccgcca acacgctcaa
     2401 agaggccatg cgccgcgcgg tagcttcacg ctcttgaacg ctacgctcgt gcagcgccgt
     2461 cttgttcacg atttcccggt gtaaagcgac gctcttcaag gccgcgccga tctgttcgcg
     2521 cagaatttcg cgcgtgccca cgccaggcaa cagctcgaac accatcacgc ccaagctctc
     2581 gtgctcgaag gtcagcggga agacatagag caagtggcac cgctcgccgc acggctcgcc
     2641 cggaggaagc aacgacgacg cacgaaaacg ggtggcgcgt ggctcgacga ggcgcccttc
     2701 actcaggcag aagaacgggc gaagctcgcg ctcgtccggg gacgcataca gagaaatgaa
     2761 cgcgttccga gctactttcg tgagctcggc ggagagcgcg gctttcagcg aggcccaatc
     2821 gaaggcggtg gagagccggt ccgcgctggc gagcgcgcgg gcgtgcgccg cctcgctcga
     2881 ccagcgccga cgcgcctgct cggcagtgct ggccgagctg atcgcgccgc gtgccgtatg
     2941 ccagaggtct tccaaacaga cccgctcgct gcgcaaacgg gcgcgcaaga gattgatgac
     3001 tccctgcagc tcttcgtaaa tttccagcgc ctccgcgtac tgggacatca gtcgctcgag
     3061 agccgcgatg aacgcgccgg cttgaccgga gagctcgcgg gccagctcgc gtagaagcag
     3121 ctcagcggcc gcgcgcgact cacgctgcgg caagcgaaac gcgcgactca gctggcgcaa
     3181 cagatcctgc gcctcggcct cgagcagcac cgccgggtca tgcgcagaat gcggccgtcg
     3241 tcgcgtctcg agctccgagc tctggcagcc gcacgaggcg cgacacagca agctgagggg
     3301 gagctcgcaa atttcatctt tcccagccga gacgccgctc ttgcccagcg cctgcgtcag
     3361 caccaagtcc accgcgcgtt ccgccatcgc atcgagcggc tgacgcacgg tcgttaggcg
     3421 cgggttggag aagcgcgagc tcggcagatc gtcgaagcca atcacgcaag ccgcctgcgg
     3481 cagtcgcaat ccagcttctt tcagcgcggc cgatgctccg acggccacgt tgtcgttgac
     3541 cgccagcacc gcgtcgaacg gtaagcctcg ggcgaggaag gcgcgcatgg cgacttgcgc
     3601 agacgccgca ccgagttcgg cgctcagctc gtactcggca ggcagctcga gtccgtactc
     3661 gccgaggacc gcacgacaag ccgcggctcg ctcgctcgcg tcctggtcgt ggtcggggcc
     3721 accgatgaag acggggcgct ggatcgagtg cgagagtacc gcgtgctcca cggcgctgcg
     3781 catgcccgcg gagttgtcaa tcaccacgct gggcaccccc ggcaccctca ccccgaagct
     3841 gcacaaagga agcccctcga agtggggtaa gagtcgctcg accgcggcca ggcccccgtg
     3901 ggctgccagg ccggacgtca ccaagatgac gccgtcgacg ctgccgcgct gaatcagccg
     3961 atacactcga tcgtggggag actccccgag cgctcttccc accacgatac acaagctgag
     4021 ctggcgagca gcgcaagcgc tcgcaaaggc gcgcctcagc tcggcttcat agccgtcgtc
     4081 cacgtggtcg acacgggcga tgagaacagc gatggttttc tgcacggcga cgagctgggc
     4141 cggcgcaacc cggcgttgct tctcgttcct gtcccgagaa tctgcctatc ctcctgagcc
     4201 gaacgacgct gtgcgacgca agtttagcgt caggggtcta cgtgaggcgt cgttttcggc
     4261 ggacgacccc ggcaaagcgt cgtataggcg ccgggcaatg agtcaccccg aacgcgatct
     4321 ctatgcacag gccggcgtgg actacggcac catggatccc ggcaagcggc gcgcccaact
     4381 cgccgcgctg cgtacggccc gcaccctgag cgaaaagggg ttgtccgagg tgacggagag
     4441 ccgcggagag agcgcctacg tggtcgattt cggggatcac tacgtgagct ccgtcaccga
     4501 agccctcggc accaagaacc tcgtggccga tgcggtacgt ccgatcaccg gccgctctca
     4561 ctacgagcag atcgggaaag ataccgtcgc gacggtgttg aacgatctct ccagcgtcgg
     4621 aggaagtccg gttagcttga ccgcctactt cggcgccgga gactccgcgt ggttccaaga
     4681 cgaggcgcgc ttcaacgacc tcatcacggg ctgggaaagc gcatgcttgg aggcgggctg
     4741 cgcctggggc gggggtgaaa cccaagtact ggtcgggatg atctcacccg agacggtggt
     4801 gctcggcggc tccgccgtcg gttgcatccg cccgaagccg cagctgctcg ccgggcgccg
     4861 cattcaagcc ggagatgcga ttctcctctt ggaagcctcg ggcatccacg ccaatggcct
     4921 gtcgctcgcg cgttctatcg cgaagcagtt gccacgaggc tatgccactc ccgtgccggg
     4981 cgatccgcaa ggtcgcgggt tcggcgaggt tttgctggat gcgacgccac tctacggtcc
     5041 cgcggtgagt gccttgcagt ccgccgacat cgagctgcac tatgcggtga acgtgacggg
     5101 gcacgggctt cgcaagttga tgcgggccga cgccgccctc agctacatcg tcgagcgcat
     5161 gcccgcgccg aaccccatct tcgagttcct gcgcgaggcg acgggtatga gtgagctcga
     5221 ggcgtacgga actttcaata tgggcatcgg ttacgctttg tatctgcggg ccgatcacgc
     5281 gccgcgcgcg ctccaagtgc tggaagcgcg gggcatcaaa gccatgctgg gcggtcacat
     5341 cgagagcggc cccaagcggg tggtactcga gccgaacggc ctcgaattct cgggagaaac
     5401 tctggccatc cgctgaatgg ccgagcgcac tgtcgaaccg gctctccttc acgaggcagc
     5461 cagatgacga acctagccca tgcaggacaa gtcgagtatg gaggcgctcg atgttgaagc
     5521 tcgggatgct cgcctctcat cgcggctcga cggtggagcg tttggtccaa gcctgccgcg
     5581 acgggcgagt gcgcggagag ccgcgcgtcg tgatcagcaa caactcgggc gcgcgcgtgc
     5641 tcgaggtggc aaaggctgca cagattcccg cgttttgtat cggaggcgcg gcatacgcga
     5701 gcgaggagct ccgtgatcgc gcgatcctcg agacgctgca gcgccacggc gtggagctag
     5761 tgttgctgct cggctacatg cgcaagctcg gccccgagac gttgagggct taccgggggc
     5821 gcgttctcaa cacccatccc gcgctgcttc cgaagttcgg cggtcaggga atgttcggca
     5881 tgcacgttca tcgagccgtg gtcgccgctg gcgaaactga atcgggcgct actctgcacg
     5941 tcgtggacga gtggtacgac catggtccag tgctgttaca gcgccgcgtc ccagttctga
     6001 ccggagatga tccggaagca ttacaacaac gagttcaggg catcgaacac gagctactcg
     6061 tggaagggct caactctatc gaatccgggc agttggtcct gcccgctccc tagacgtagc
     6121 tgttttcgaa cacgactcga ggccggccga gcgcacttct gcccgcccat ttctgcgatc
     6181 cgatttgctt gtcgcgcgcg gtcgccgagg ttgcccggcc agatggccag cgctaagctc
     6241 cgccccgtcc tgagttcctg agtttggaga cgtatcggta tgaaatcaga tgtgaccgtg
     6301 gtgtgcggcg tgaattgggg cgacgagggc aaaggccgga tggtggatta cctcgcggaa
     6361 gacgcccaaa tcgtcgcgcg ttttcagggc ggcaataacg cgggacatac gatcatcaac
     6421 cagtacggtc acttcgccct gcatctcgtg ccctccggca tcttccgtcc ttccgtctac
     6481 aacctgctcg gtcccggcat ggtgatcgac ctcgaaggtc tggtggacga gatggcccgc
     6541 ttgaatcagc gaggcgtcag caccgaacga ttgctgctct ccgatcgcgc caccgtgtct
     6601 ttcccctttc accgcaagct cgatgagtgg gaggagaaac gcttggcggg ccgcgcctac
     6661 ggctcgacca agaacggcat cgcgcccgcc tacggcgatc gtcacttgaa gaaggcggtc
     6721 cgcatcgggg acctgttcga tcgcgatggt cggcgcgacg cgatcgcgcg cgcggtcgag
     6781 tgggccaacc tgctcgcccg ggacgtgtat cacgaggatg gtttcacaga cgcgcagatg
     6841 ctcgcctggt gcgatcagtt cttgccgcgc ctcgagccgc acgtcaccga cacggtgacg
     6901 tttctcgaga acgcggtgca agagggcaag aacgtgctgc tagaggccca gctcggtgcg
     6961 ctgcgtgacg tgtacttcgg catttaccca tacaccacgt cgtcttgtgc gctctccgcg
     7021 ttcgccccca tcggtgccgg cctgttcggc gtcaaagcca accgcgtggt gggggtgatg
     7081 aaagccttcg ccacgtgcgt aggtgaaggg ccgttcgtca cccgtatgac ggacgaggaa
     7141 gcgtccgagc tgcgagagtc tgccaaggag tacggagcac gcaccggccg tccacggacg
     7201 atcggtcact tcgatgcggt ggcctcgcgc ttcggcgccc aggtgcaaga caccaccgag
     7261 ctcgcgctca ccaagctcga tagcttgtcc gggcacaaag agctgaagct ttgcacggcc
     7321 tacaacgtgg acggcaagca gacggagcgc ttccccgtga ccggcttgta ccgcgcgacc
     7381 ccggt
//
LOCUS       DASTKU010000422        14094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1864528, whole genome shotgun sequence.
ACCESSION   DASTKU010000422 DASTKU010000000
VERSION     DASTKU010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14094
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864528"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /gene="xseA"
                     /locus_tag="VFQ61_14470"
     CDS             complement(<1..856)
                     /gene="xseA"
                     /locus_tag="VFQ61_14470"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET9955715.1"
                     /translation="MPNEDKTQHGPKRRVVLAQSGGPTENRTPAINPWRAFGIPCYTR
                     RAVSGGPSETVLSVAELGRQLKNATESLGLSLWVEGEVSSFKRAPSGHVYFTLKDQAA
                     DACIECVMYKLDAFRSLRHLAEGAKIQLCGRATVWAPRGRLQFVGHRARPAGRGALLI
                     ALEALKEKLAQEGLMDPARKRALPREPRVVGIVTSAAGAAFHDIRSVAFRRGAVKLVL
                     SSALVQGDGAPESLLKALDRIERYPGLDVVIVGRGGGSGEDLMAFNDERVVRRVAGFA
                     VPVVSAVGH"
     gene            973..2451
                     /locus_tag="VFQ61_14475"
     CDS             973..2451
                     /locus_tag="VFQ61_14475"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET9955716.1"
                     /translation="MFRKVLIANRGEIAIRIARTLREEGIASVGVFTEADHESPFIDL
                     VDEAVELGANARAYLDVDAIVGAARRVGANALHPGYGFLSENADLAEACEQAQITFVG
                     PSPRAIRAMGSKQRARQLMSQAGVPIVPGGDADTFERARESAARVGYPVLLKATDGGG
                     GKGMRRVDSEAELAAALERTQSEAEASFGSRRVHVEKALLNARHVEIQVLGDRHGRLV
                     HLYERDCSMQRRHQKIIEETPCPVLPEGTLDRMAEVALAGARALGYYSAGTFEFLLDA
                     SGEFYFLEMNTRLQVEHPITELVTGLDLVREMLRIAAGQALPDELPARRGAAIEARIY
                     AEEPARGFLPSPGRISHLREPSGPFVRIDSAIRSGTLVGTDYDPLLAKLSVWAPDRET
                     ARRRLLRALSEYRVGGLQTNLGYLMRLVQSPAFVAGEYDTRWVESNRQLWAEPALSAE
                     LGDALSAAICALESQSSNPERTRSPTVSGTSPWVLQERARLR"
     gene            complement(2460..3434)
                     /locus_tag="VFQ61_14480"
     CDS             complement(2460..3434)
                     /locus_tag="VFQ61_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955717.1"
                     /translation="MQPTRVRLGELLVEAQIITRDQLQQVLDRQKSDSRRLGTLLVEA
                     GFVTETQVTQILSQQLSVPWVSLYHVDFSRQLLNLVTEELADKYCLVPIFVRRVRGVG
                     ETLYVAMDDPSDEGALKEVAQYSGLSVRAMIAPPSDIRAAIRVYYNKGADSSSLSDAV
                     REFGTHSAPPERDAQSTSPPPAGQAFGAAGSLVQPPPSPVLQATAAEDEPPDSGPQIE
                     AREVSMPAPKRGRPRKMVTLTLLDGTQINLPAKPRREPSEPAPKDAANLTARDIVAAL
                     RAVSHGADASEILGGSVRWEAFFAALLSVMMKKHLIADWEFVDEFRKV"
     gene            3724..6390
                     /locus_tag="VFQ61_14485"
     CDS             3724..6390
                     /locus_tag="VFQ61_14485"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9955718.1"
                     /translation="MSSTSEAIRPCPHCGSSCAVQHQYCPTCGFPVGTVNTANDDRFV
                     GKTLPGGYHILDLISVGGMGRVYRAQQSALGRTVAVKIIHPHLLSDENSALRFMTEAR
                     AASQLNHPNSVAVFDFGRTDDGQPYLVMEFLRGKDLARVAYEEGALPFPRIVDVLRQV
                     LAALGEAHDLGIVHRDLKPENVILEPLRRGGDFVKVVDFGLAKLRADTQQPGVTSPGI
                     VCGTPDYMAPEQGRGDPIDGRSDLYAVGVMFFQLLTGRLPFEADSPTQVVMMHLTVPV
                     PDPRQVAPERHIPDSLVEVALKALAKDNRKRYQDAQEFSDALTRALTIAESVPPERAS
                     LPPQSIHAATVTCSACGQSVPPAKFCGECGARLPIRSGIPDGAAPVFPLPFVAREEDL
                     NWLDDKRRQLAEELVAARVVGEPGSGKSRLLAELLSLAKSENDAVAAAGPDPYHAEVA
                     YYALREAIKQLARLSPNDIEAGRFERVRPEARAGLDEIFGRQAHNDGAHSLFDRRETL
                     AEALRWALCLAANRSRRVLLTVDDIDDIDGPSRNALLDVISDPPVAAVLLVVSHRPGF
                     DASPLGPRTSPRMLGGLPPPAVSRLLAQRPRMERSLLGEGGKGVLPMYVDQVLRYSLE
                     GGSDPPQRLADLIALRVDTLESNARRTLQALAVLGDRVPAAVVAELLPRSELPPGSIE
                     DLVQKGMIERADGKLSCSHPLLREIVLGGIPVAVRRELHKKAIAVSEKLAQPLEACAA
                     HAYESGDSFQALVLLEQMAGRASQRGDSRTEVLCLRRCVEVARKEISRGELDDPLRAL
                     LIFSRKLGGALTRAGNFADAEGVLREAMDIAGPSGPDRARLLSALAHVAHGRKRDHEA
                     FTFLEQAIELARQSGAHELVANLSDTRRVWGI"
     gene            6422..7552
                     /locus_tag="VFQ61_14490"
     CDS             6422..7552
                     /locus_tag="VFQ61_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955719.1"
                     /translation="MTDESKRFITRWDLDKTYLRTSFDSLSDLLNTALESVEDKRTVP
                     GATAVLRELGEAGGTIHIVSGSPRQMRKRLTQKLELDRVVFRELTLKPNLSNLLRLRF
                     GALRDQLGYKLPELLEAHARETRDAAGPALPEVLVGDDSESDAFIYSLYADLCAGKID
                     SSELSEVLRVGKVREHSARRCLRALQELKRADNVAVILIHLDGQSPPSLFRPYGPRVV
                     PFFNYLQAAFVLLERGFLSARQVVAIAYDFHAHHGFTADVMARSYADLYRRGHADGSA
                     PARLLPALGGFHSPRVTEATSVSTPSPSEASEEEAPPLSLNSALWEAVREMQQHTAPG
                     PLLASNASKDGPPKLEPKLDYRVLAENHRGGQNRRKPGQRFR"
     gene            7807..8550
                     /locus_tag="VFQ61_14495"
     CDS             7807..8550
                     /locus_tag="VFQ61_14495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HET9955720.1"
                     /translation="MKTVERIQIGQLPVDVVDLNGALDVIDELVANGGGTVFTPNVDH
                     VVVAEQNARFRAAYSRASLSLVDGMPVLWAARLLGQPLPMKVSGSDLIEPLMQRAAQR
                     GYRVYLFGGDDGVAEKARDVLIQKFPGLNIVGWDNPRINVDAISDELLAKIRATNPQL
                     VLVALGAPKQELFCAEQREKLAPAVLLGIGASLDFVAGIRKRAPRWMSRMGLEWLFRL
                     AQEPRRLAHRYLVRDPQFLWVLGKQIAGK"
     gene            complement(8696..10045)
                     /locus_tag="VFQ61_14500"
     CDS             complement(8696..10045)
                     /locus_tag="VFQ61_14500"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HET9955721.1"
                     /translation="MGRTEHSRITAEFEGVELGDRRRNARLLLLAERLAEQPDASFPD
                     AMSNEAELEAAYRFFNNVKVEAAEILRPHVRQTVERAKALPVALVAHDSSTVSFDSEY
                     REGLTRRGSKQHFIVHCSLAMHADGSRRPLGLLAMSQHLPVSTMNKALQDRWAEHVRE
                     VHAHGLSPDNVVHLMDREGDDYEVLDLLKRLQTRFVLRVQHNRVLKNGTLRDSLANTQ
                     LRAEREVELSRRTAKESGPKQSKIHPPRENRLARLAIAAGSVTIPRSMSAKSSCAESL
                     SLNVVRVWEPSPPEGQAPVEWLLYTSEPIETPEQILQVVDWYRARWTIEEYYKALKTG
                     CALEKRQLGDLHALSNALALLAPIAWQLLLLKTESRVHPDAPATSMLDEDELRVLRLV
                     SQRRKLPDNPTMLDAMLAIAGLGGHLKHNGPPGWITLARGYEKLRNLTTGLRLAQST"
     gene            11341..13737
                     /gene="ppsA"
                     /locus_tag="VFQ61_14505"
     CDS             11341..13737
                     /gene="ppsA"
                     /locus_tag="VFQ61_14505"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HET9955722.1"
                     /translation="MASKERYVLAFKEVGLGDVALVGGKNASLGEMLQAFQAKGIRVP
                     DGFAVTVAAFREHISQNGLDGEIYAALDALDVKDVSALARVGAWIRERVRSVPLPEQV
                     KREVLDAYRALSEAYGESQTDVAVRSSATAEDLPTASFAGQQETYLNVRGAATVLDAV
                     RDCMASLFTDRAIVYRAERGFKHSAVALSVGIQKMVRSDLGSAGVIFTLDTETGFRDV
                     VLVTGAYGLGETVVQGRVNPDEFWVHKSTLLEGFAPIVRRERGEKAVRLVYAQGSTKS
                     VVEERVPRDLREQYVLSEAEVLELSRWALSIESHYSERHGRPTPMDIEWAKDGRTSEL
                     YIVQARPETVHAQAGPPRFEMYRLRGKAPLLVRGKSVGAKVTSGPVRVIHSASELASF
                     KPGEVLVAPMTDPDWEPVLKQAAAVVTDHGGRTCHAAIVSRELGIPCVVGSGSATQVL
                     ETGRVVTVSCAEGDEGHVYQGVVPFSREEIEPSKLPRPRVPVMLNVGNPDLAFLVGQL
                     PSAGVGLLRMEFVVSSWISVHPMALIHPERVEDPSVTAKIRARTAGYERPTEFFVERL
                     ASGVAQIAAAFYPRPVIVRMSDFKTNEYSGLLGGEYFEPVENNPMIGFRGASRYYDER
                     YREGFALECQALLRVRERMGLRNVKVMIPFCRTLEEGRRVLAELDRNGLRRGDNGLEV
                     YVMCEIPNNVVLAEEFSELFDGFSIGSNDLTQLMLGVDRDSELLAHVFDERDPGVKRM
                     IELVIERAHKRGRKVGLCGQAPSDHPEFARFLADAGIDSISVTSDALPKVVRILAKPV
                     "
     gene            complement(13747..>14094)
                     /locus_tag="VFQ61_14510"
     CDS             complement(13747..>14094)
                     /locus_tag="VFQ61_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9955723.1"
                     /translation="KLSGGQRQRIALARAILKNPPVLVLDEATSAVDNETEAAIQRSL
                     DKLVVGRTTLIIAHRLSTVRHADVIYVLEGGRIVEAGTHESLIAEGRQYSMLWRLQTG
                     ELPSSLPAAHPAN"
BASE COUNT         2465 a         4442 c         4627 g         2560 t
ORIGIN      
        1 cgtgcccgac cgcactcacg acggggaccg cgaagcctgc cactcggcgc accacgcgct
       61 cgtcgttgaa ggccatcagg tcttcgcccg aaccgccacc gcgaccgacg atcaccacgt
      121 cgagccctgg gtaacgctcg atacgatcga gcgccttcag caagctctcg ggggcaccat
      181 cgccttgcac gagcgcggac gataacacga gtttcaccgc gccgcggcga aacgcgacgc
      241 tccgaatatc gtgaaacgcg gccccggcgg ccgacgtcac gataccaact acacgcggct
      301 cacgcggcag cgcgcgctta cgcgccggat ccatcaggcc ctcttgagcg agcttctcct
      361 tcagggcctc gagcgcgatc agcagcgcac cccgcccggc cggacgcgcg cgatgcccga
      421 cgaactgcag ccgcccgcgt ggcgcccaca ccgtggctcg cccgcagagc tgaatcttcg
      481 cgccctccgc gagatgccgc agtgaccgga aagcatcgag cttgtacatg acgcactcga
      541 tgcaggcgtc cgcggcttgg tccttcaacg tgaagtacac gtgcccgctc ggagctcgct
      601 tgaaagagct gacctcgccc tcgacccaca gcgaaagccc gagtgactcg gtggcgttct
      661 tcaactggcg cccgagctcg gctacgctca aaacagtttc agagggccca ccgctcacgg
      721 cgcggcgggt atagcagggg atcccgaagg cacgccatgg gtttatggcg ggggttcgat
      781 tctccgtggg cccgccgctt tgcgcgagca cgacccggcg cttcgggccg tgttgagttt
      841 tgtcttcatt cggcacgcgc cagtggcatg ctgggccggc tcggcgggcg ccggccacca
      901 ggtgcggcac gttcgatgcc agccgaggcc ccaaccggtt tcgaagtgct cccatgataa
      961 aaacggctcg gcatgttccg taaagtcctg atcgccaatc gcggggagat cgccatccgc
     1021 atcgcgcgca cgctgcgcga ggagggcatc gcgtcagtgg gggtgtttac cgaagccgac
     1081 cacgagagcc ccttcatcga cttggtcgat gaagcggtag agctcggagc gaacgcgcgg
     1141 gcctatctcg atgtcgatgc cattgtgggc gcggcgcgcc gggtgggcgc gaacgcgcta
     1201 catccaggct acggctttct gtccgagaac gccgatctgg cagaggcgtg cgagcaggcc
     1261 cagatcacgt tcgtcggccc ctcgccgagg gcgattcgag cgatggggag caagcagcgc
     1321 gcgcgtcagc tcatgtccca agcgggcgtg ccgatcgtgc ccggagggga cgctgacact
     1381 ttcgaacgag cacgcgagtc cgcggcgcgc gtgggttacc ccgtgttgct caaggccacc
     1441 gatggcggcg gcggcaaggg catgcgtcgc gtggatagtg aagccgagct cgcggccgcg
     1501 ctcgagcgca cgcagagcga ggccgaagcc agctttggca gccggcgcgt gcacgtcgag
     1561 aaggcgctgc tcaacgcgcg acacgtagaa attcaggtac tcggagatcg acacgggcgg
     1621 ctcgtgcatt tgtacgagcg cgactgctcg atgcagcggc gtcaccagaa aatcatcgag
     1681 gagacgcctt gcccggtgtt gccggagggc accctcgacc gtatggcgga ggtagccttg
     1741 gccggcgcgc gagcgctcgg ttactactcc gcgggcacct tcgagttcct gctcgatgca
     1801 agcggtgagt tctactttct cgaaatgaac actcgcctgc aggtggagca cccgatcacc
     1861 gaactagtga cgggcctcga cttggtgcgg gagatgctgc gtatcgcggc ggggcaggcg
     1921 ttgccggatg aactgcccgc tcgacgcgga gccgcgatcg aagctcggat ttacgccgaa
     1981 gaacccgcac gcggcttttt accgagcccg gggcgcatca gtcatttacg tgagccgagt
     2041 ggccccttcg tgcgcatcga cagcgccatc cgcagcggca ccctggtcgg cactgactat
     2101 gatccgctgc tcgccaagct gagcgtatgg gcccctgacc gagagacggc gcggcgccgg
     2161 ttgctgcgcg cattgagcga gtaccgggtt ggcgggttgc aaaccaatct gggatatctg
     2221 atgcggctcg tccaaagccc cgccttcgtc gcgggtgagt acgacacgcg ctgggtcgaa
     2281 tccaaccggc agctgtgggc ggagccggcg ctatccgccg aactggggga cgctttgagc
     2341 gccgccatct gtgcgctcga aagccagagc tcgaacccag aaagaacgcg ctcccctact
     2401 gtctccggca cgagcccctg ggttctgcag gagcgtgcgc gcctgcgttg acgcacccgt
     2461 cacaccttgc ggaactcgtc cacgaactcc cagtccgcga tcaggtgctt cttcatcatc
     2521 accgagagca gcgcggcgaa gaaggcctcc cagcgtacgc tcccacccag gatttcgctc
     2581 gcgtccgcgc catgactcac ggcgcgcagc gccgccacga tgtcacgcgc cgtcaggttc
     2641 gcagcatctt tgggcgccgg ctccgagggt tcacggcgcg gcttggcggg tagattgatc
     2701 tgagtgccgt ccaacagcgt caacgtcacc atcttgcgcg gtcggcctcg cttgggcgcc
     2761 ggcatcgaca cctcgcgagc ttcgatctgg ggcccgctat ccggcggctc gtcctctgcg
     2821 gccgtggctt gcaagaccgg tgatgggggc ggctgcacta gcgatcccgc ggctccgaat
     2881 gcctgcccag ccggcggcgg cgaggtcgat tgcgcatccc gctcaggggg ggcgctgtgg
     2941 gtaccgaact cgcgcaccgc gtcggacagt gacgagctat ccgccccctt gttgtagtaa
     3001 acgcggatgg cggcgcgaat gtccgagggg ggcgcaatca tcgcgcgcac gctcaacccg
     3061 gagtactggg ccacctcctt cagcgcacct tcgtcgcttg gatcatccat cgccacgtag
     3121 agcgtctcac caaccccgcg cacgcgacgt acgaagatgg gaaccaggca atacttatcc
     3181 gccagctcct cggtgaccaa gttcaagagc tgacgcgaga agtcgacgtg gtagagcgat
     3241 acccaaggca cgctcagctg ctgactcaaa atctgagtga cctgcgtttc agtcacgaaa
     3301 ccggcctcga cgagcagcgt gcccagccga cgactgtcgc tcttctgccg atccaacact
     3361 tgctggagtt ggtcccgagt gatgatctgc gcttcgacga gcagctcgcc gagccgcacg
     3421 cgcgtgggct gcatttgaaa cactctaaaa cagttccatt gcgaatggcg cgagcggttt
     3481 ctctcttggc caaagggcac tcccacttgg ggggccgacc gacctacaat cgaaacctcg
     3541 acgaaaaccg tcacctgcta gaaagtagcg tcaagactgt tgaaactgcg tagcgcggaa
     3601 tagactcaat ccttcacgag cccactgcgg gcctggctcg cggcgggccg gccctggtcc
     3661 gtcccgctgg cgccgcagac cctcgaaacc ggcgtcgaac tcattctgtt agccctgccg
     3721 cacgtgtcct cgacttccga agccattcgc ccctgtccgc actgcggctc gtcgtgtgca
     3781 gtccagcacc aatactgccc cacgtgtggc ttcccggtcg gaacggtcaa taccgcgaat
     3841 gacgatcgtt tcgtgggcaa aacactaccg ggtggctatc acatcctgga cttgatcagc
     3901 gtcgggggca tggggcgcgt gtaccgtgcc cagcagagcg cgctcggccg caccgttgcg
     3961 gtcaagatca tccacccgca tctgctctcc gacgagaact cggcgctgcg cttcatgacc
     4021 gaagcgcggg ccgcgagcca gctcaatcac cccaactcgg tggcggtgtt cgacttcggc
     4081 cgcaccgatg acgggcaacc ctacttggtg atggagttct tgcgcggcaa ggacctcgcg
     4141 cgcgtcgcct atgaagaagg cgcgctgccg ttcccgcgga tcgtggacgt gctgcggcag
     4201 gtgctcgcgg cgctgggtga agcgcacgat ttgggcatcg tgcaccgcga tttgaagccc
     4261 gaaaacgtga tcctcgagcc actccggcgc ggtggagact tcgtcaaagt agtggatttc
     4321 gggctggcca agctgcgcgc cgatacgcaa cagccaggcg tcaccagccc cggcatcgtg
     4381 tgcggcacgc ccgactacat ggcgcccgag caggggcgcg gagatccgat cgacgggcgc
     4441 agcgatctgt atgccgtcgg ggtgatgttc ttccagctgc tgacgggtcg tctgcccttc
     4501 gaggccgaca gccccacgca ggtcgtgatg atgcacctca cggtgcccgt gcccgatccg
     4561 cgccaagtag cacccgagcg tcatatcccg gattcactgg tggaagtggc actcaaggcg
     4621 ctcgccaaag acaaccgtaa gcgttaccag gacgcgcaag agttctcgga cgcactcacg
     4681 cgggccctca ccatcgccga gagcgtgccg cccgagcgcg cttcgctgcc accccagtcg
     4741 atccacgccg cgaccgtgac ttgctcggcg tgcgggcaga gcgtgccccc cgccaagttc
     4801 tgcggtgagt gcggcgcgcg gctgccgatt cgctcgggca ttccggacgg cgcggcaccg
     4861 gtttttccgt tgccgttcgt ggcgcgggag gaagatctga actggctcga tgacaagcgc
     4921 cgccagctgg cggaagagct cgtcgccgcc cgcgtcgtgg gcgagcccgg ctcgggaaag
     4981 tcgcgcttgc tcgccgagct cctgtccctg gccaagagcg agaacgacgc cgtcgcggcg
     5041 gcggggccag atccttacca cgcggaggtg gcgtattacg ccctgcgcga agccatcaaa
     5101 caattggcgc gcctcagccc aaacgacatc gaggcaggcc gtttcgagcg cgtgcgcccg
     5161 gaagcacgcg caggcttgga cgagatcttc gggcgccaag cgcacaacga cggcgcgcac
     5221 tcgttgttcg accgccgcga gactctggcc gaagccctcc gctgggcgct gtgcctggcc
     5281 gcgaaccgct cgcggcgggt cttgctgacg gtcgacgaca tcgacgatat cgacgggccg
     5341 agccgcaacg cactgctgga tgtgatttcc gatcctccgg tcgccgccgt actgctcgtg
     5401 gtctcgcacc ggcccgggtt cgacgcgagc cccctcggcc cgcgcacttc gccgcgcatg
     5461 ctcggtggcc tgccgccacc ggctgtctca cgcctgctcg cgcagcgccc gcgcatggag
     5521 cgcagcctgc tcggagaagg tggcaagggc gtgctgccga tgtacgtcga tcaggtgctg
     5581 cgctactcgc tcgagggtgg cagcgatccg ccgcaacgcc tggccgatct gatcgcgctg
     5641 cgggtcgata cgctcgagtc caacgcgcgt cgcacgctcc aagccttggc cgtgctcggc
     5701 gaccgagtgc cggcggcggt agtagcagag ctcttgccgc gctcggagtt gccgcccggc
     5761 agcatcgaag atctggttca aaagggcatg atcgagcgcg ccgacgggaa gctgagctgc
     5821 tcgcatcccc tgttgcgcga gatcgtattg ggaggcatcc cggtcgcggt gcggcgtgag
     5881 ctgcacaaga aagccatcgc ggtgagcgag aagctagctc agccgctcga ggcctgcgcc
     5941 gcgcacgctt acgagagcgg cgactcgttt caggccctgg tgctgctcga acaaatggcg
     6001 gggcgcgcca gccaacgcgg ggactcacgc accgaagtgc tgtgtctgcg ccgctgcgtg
     6061 gaggtcgcgc gcaaggaaat ctctcgcggt gagctggacg atccgctgcg ggcgctgctc
     6121 atcttcagcc gcaaactggg cggcgcattg actcgggccg gcaacttcgc ggatgcggag
     6181 ggcgtgttgc gcgaggccat ggacattgcg ggcccgagcg ggcctgatcg cgcgcgattg
     6241 ttgtccgcct tggcgcatgt cgcgcacggt cgaaagcgcg accacgaggc cttcactttc
     6301 ctcgagcaag ctatcgaact ggcgcgtcaa tcgggtgctc acgagctggt tgcaaatctc
     6361 tcggacacac ggcgcgtttg ggggatttga agcgccagga cgcgagcgag ggagcgccga
     6421 ggtgaccgac gaatcgaagc gcttcatcac gcgctgggat ctcgacaaga cgtaccttcg
     6481 gaccagcttc gacagcctct ccgatttatt gaacaccgcg ctcgaatcgg tagaggacaa
     6541 acgcacggtt ccgggtgcga ccgcggtgct gcgcgagctc ggtgaagccg gcggcacgat
     6601 ccacatcgtg agcggcagcc cgcgtcaaat gcgcaagcgg ctcactcaaa agctcgagct
     6661 cgatcgtgtg gtgtttcgag agctgacgtt gaaaccgaat ctctcgaacc tattgcggct
     6721 gcgttttggg gccctgcgcg atcaactcgg gtacaagctg cccgagctct tggaggccca
     6781 tgcgcgagag acccgggacg ccgcggggcc cgcattgccg gaggtcctcg taggcgacga
     6841 ctccgaatct gacgccttca tttactcgct gtatgcggat ctgtgtgccg gcaagataga
     6901 ttccagcgaa ctcagcgagg tgctgcgcgt cggcaaagtg cgagagcaca gcgcgcgccg
     6961 ctgcctgcgc gcgctgcagg agctgaaacg agccgacaac gtcgcggtga ttttgattca
     7021 tctcgacggg caatccccgc ccagcctgtt tcggccgtac gggccgcgag tcgtcccctt
     7081 cttcaactac ctccaggcgg ccttcgtcct cttggagagg ggcttcttga gcgctcgcca
     7141 ggtggtggcc atcgcgtacg acttccacgc ccatcacggc tttaccgctg acgtcatggc
     7201 gcgcagctac gccgacctat atcgacgcgg ccacgccgac ggcagcgccc ccgcgcggct
     7261 tctacccgcg ctcggcgggt tccattcgcc ccgagttacc gaagccacgt ccgtctcgac
     7321 cccctcgccg agcgaggcct ccgaagagga agcgcctccc ctttctctca actccgcgct
     7381 gtgggaagcc gtgcgcgaaa tgcaacagca cacggccccg ggcccgctct tggcctcgaa
     7441 tgcctccaaa gacggcccgc ccaagctcga gcccaaactc gactatcgcg tgctcgccga
     7501 aaatcatcgc ggtggccaga atcggcgcaa acccggtcaa cgtttccgct gagaacctag
     7561 cccagtaatt cggataggtt ctaagagttc gcgtcggctc gctcgtcact cgattgcctg
     7621 cagtagaatg tgcgggctgg ccattgcata acgtgcgcgc tcggccactc ccctcgacac
     7681 cgtgggccag cccgccgcgc gatcccccgc accgcagccg cgcctgcccg tcgcaataac
     7741 gcgagccgct tccctctcgg gtcgcaatcc gctatcgaga aagcgggaga ctttgcgaga
     7801 aggcacatga aaacggtcga gcgtatccag attggtcagc tacccgtcga tgtggtcgat
     7861 ctcaacggcg cgctcgacgt catcgacgag ctcgtcgcca atggcggcgg cacggtgttt
     7921 acacccaacg tcgaccacgt cgtggtcgcc gagcaaaatg cgcgatttcg ggcggcctat
     7981 tcacgagcga gcttgtcctt ggtcgacggc atgcccgtac tctgggcggc gcgtctgctt
     8041 gggcagcctt tacccatgaa ggtgtcgggc tctgacttga tcgagccgct catgcagcgg
     8101 gccgcgcagc gcggttatcg tgtctacctg ttcggcgggg acgatggcgt ggccgagaag
     8161 gcgcgtgacg tcctgatcca gaaattcccg gggctcaaca tcgtgggctg ggacaaccct
     8221 cgcatcaacg tggatgcgat ctcggacgag ctgctcgcca agatccgcgc gaccaatcct
     8281 caattggtgc tggttgcgtt gggcgcgccc aagcaggagc tgttttgcgc cgagcagcgg
     8341 gagaagttgg cccccgcggt gctgctcggg attggcgcga gcttggattt cgtggccggc
     8401 atccgcaagc gcgcgccgcg ctggatgtcg cgcatggggc tcgagtggtt gtttcggctg
     8461 gcgcaagagc cgcgtcgact cgcgcatcgc tacttggtac gcgacccgca attcctgtgg
     8521 gtgctcggga agcaaatcgc ggggaagtga agcttgtttt tttgggaggg tctgatgcag
     8581 accctccccc cgtcggaagt gaattccgcc ggggaccccc ctcccaaccc gctggcgctg
     8641 cgcgcgcggg gccccagcct catcattgat cgcggaatag tcgaacaatt ccaatttacg
     8701 tggactgcgc taagcgcagt ccagtggtta gattccggag cttctcgtaa ccacgggcca
     8761 gcgttatcca accgggtggg ccgttatgtt tgaggtgccc gccaaggcca gcaatcgcga
     8821 gcatagcgtc tagcatggtt gggttgtcgg gcagtttgcg ccgctgagaa acgagtcgga
     8881 gtacccgcag ctcatcttcg tcgagcattg aggtggccgg agcatctggg tgtacgcgtg
     8941 actcggtctt caagagcagg agctgccaag caattggcgc gagcagcgcc agtgcattgc
     9001 tcaaggcgtg gagatcacca agctgacgct tctcgagtgc gcaaccggtc tttaatgctt
     9061 tgtagtactc ttcaatggtc caacgcgctc tgtaccaatc cacgacttgc aagatctgtt
     9121 cgggcgtctc aatcggctcg ctggtgtaga ggagccactc cacgggagcc tgtccttcgg
     9181 gtgggctcgg ctcccatact cgtacgacgt tcagcgatag cgattcagca cagctgctct
     9241 tcgcgctcat tgatcgcggg atcgtcacgg aacccgcagc gatggccaag cgagctagcc
     9301 gattctcgcg tggtggatgg atcttgcttt gcttgggtcc ggattctttc gctgtgcgac
     9361 gagagagctc cacctctcgc tctgctcgca gctgggtgtt cgccagtgaa tctcgaagcg
     9421 tcccgttctt cagaactcga ttgtgctgga ctcgtagtac gaagcgagtc tgcaaacgtt
     9481 tcagcagatc caacacctcg tagtcgtcgc cttcacgatc catcaagtgg accacgttgt
     9541 cgggagacag tccgtgcgca tgaacttcgc gcacgtgttc ggcccaacga tcttgcaagg
     9601 ccttgttcat cgtgctcaca ggcaggtgct ggctcatcgc taagagccca agtggtcggc
     9661 gcgacccatc cgcgtgcatc gctagcgagc agtggactat aaagtgttgc ttgctccctc
     9721 tgcgcgtcag cccttcccgg tactctgaat caaacgatac cgtcgatgag tcgtgagcaa
     9781 ccaacgcgac cggcaatgcc ttcgcacgct cgacggtttg tcgaacgtgg gggcgaagga
     9841 tctccgcagc ctctactttc acgttgttga agaatcggta cgccgcttcc agctcggctt
     9901 cgttcgacat cgcgtcagga aagctggcat ctggctgctc cgccaatctt tccgcaagca
     9961 aaagcagccg agcatttcgg cgacgatcgc caagctcaac tccctcaaat tccgccgtga
    10021 tccgcgaatg ttcagttctt cccacccgca gctttgatca tgctcagccg aatctttcaa
    10081 gaagatacga ccaatccata ggccccagcc ccgcttgcgg acgacgctct cgcgagcgct
    10141 gcgcgcgggg ccccagcccc gcttgcgaac ggcgctctcg cgagcgctgc gcgcggggcc
    10201 ccagccccgc ttgcgaatga cgctctcgcg agcgctgcgc tttcgaacgg cgcagtcgtg
    10261 aggcgctgcg cgcggggcca cctcccaacc cgcgagagcg cgcgcggggc cccagccccg
    10321 cttgcgaacg acgctctcgc gagcgctgcg cttgcggacg gcacactcgt gaggcgctgc
    10381 gcgcgtggcc cgagccccgc ttgcgaacag cgcacttgcg agcggtgcgc gcgggcccta
    10441 gctccgcttt cgaacggcgc agtcgtgagg cgctgagcgc gtggccgcgt accaacccgc
    10501 gagcgctgag cgcggggcct tagcctcgct tgcgaacggc ggagtcgtga ggcggtgggc
    10561 gcgggggcct agcccggctc gcgatgggcg agatgatgag tgatccgact cgcgaggtgc
    10621 caggctcatc gttgatcgcg gaacagtcga acagttccaa cttacaagga ccgcgctaag
    10681 cgcagtccag tggttagatt ccggagcttc tcgtggccgc gggccagaac tatccaaccg
    10741 ggtgggccgc taggtttgag gtgcccggca aggcacgtgt ggccgcgagc agggcgtcta
    10801 gaattggtgg gttggcgggc agtttgtgct gctgagcaag gaggcaggag tacctgcagc
    10861 tcatcttcgt cgagcatgga actggccgga gcgtgtaaga gccaagcgac gcggtgggac
    10921 tccgagcgcc cggaccacga atgggttcaa tcgagattgg gttcgagtgc ggctgtcggg
    10981 agttcagtgg gtgcgttgca gagtattgga gtcgggtggg gggggggggc ggagtgatgg
    11041 ttggtcaatt gcgcggttcg agtgcgtttg ctttggccgg actgctatga cggcataggt
    11101 gagtgggcgt ttaggtgctg gtcgtgaatc gcaatagggt ctgggcggcg aaagttggat
    11161 tgggcatctc gtgatgcaga agtcttgccg gtgggcatga ccggcgccgg gtgcgtggag
    11221 gggaggggag gccaataatg ggagtgtctg cgttgtcttg cgggcgcgct ttgtcctgcg
    11281 tgcagtgcag acgagcttcg gctgcgagcg tgaggctcga agcgcgaaag gagtgaacac
    11341 atggcgtcca aagagcgtta cgtgttggcc ttcaaggaag ttggcctagg tgatgtcgcg
    11401 ttggtcggcg gcaagaacgc atctctcggt gagatgctgc aggcgtttca agccaagggc
    11461 atccgggttc ccgacgggtt cgcggtcacg gtggccgcgt ttcgtgaaca catctcgcag
    11521 aacgggctcg atggcgagat ctacgctgcg ctggatgcgt tggatgtaaa ggacgtttcg
    11581 gcgttggccc gagtcggtgc ttggattcga gagcgcgttc gctcggtgcc attgcccgaa
    11641 caggtcaagc gtgaagtgct ggatgcttac cgggcgctgt cggaggccta cggcgaatct
    11701 caaacagatg tcgcggttcg ctccagcgcc accgcagaag acctgccgac ggcctcgttc
    11761 gcgggtcagc aagagacgta tctaaacgta cgcggcgctg ccacggtgct ggacgccgtt
    11821 cgggactgta tggcgtcgct cttcacggac cgcgcgatcg tgtaccgcgc cgagcgcggc
    11881 ttcaagcact cggcggtggc cctctcggtg gggattcaaa agatggtgag gagtgacttg
    11941 ggatcggcgg gagtcatttt cacactcgat accgagactg gttttcgaga tgtcgtgctg
    12001 gtgacgggag cttacgggtt gggagagacc gtggtgcaag gccgcgtgaa cccggacgag
    12061 ttctgggtgc acaagtcgac attgctggag gggttcgctc cgatcgtgcg ccgcgagcgc
    12121 ggtgagaaag cggtccgttt ggtctacgct cagggctcaa cgaagagtgt cgtcgaggag
    12181 cgcgtgccgc gcgacttgcg ggagcaatac gtgctgagcg aagcggaggt gctcgagctc
    12241 tcgcgctggg ctttgagcat cgaatcgcac tactccgagc ggcacgggcg cccaacgccg
    12301 atggatatcg aatgggcgaa ggacggccgc acttccgagc tttacatcgt gcaagcgcgt
    12361 ccggaaacgg tgcacgctca ggccggtcca ccgcgcttcg aaatgtaccg attacggggc
    12421 aaagccccgc tcttggtccg cggcaagagc gttggcgcga aggtcacgtc gggccccgtc
    12481 cgcgtcatcc actcggcttc tgagctcgcg tcgttcaagc cgggtgaagt gctggttgca
    12541 ccgatgacgg atcccgattg ggagcccgtg ctcaagcagg ccgccgcggt ggtgaccgat
    12601 cacggtgggc gcacctgtca tgcggccatc gtctcgcgtg aactcgggat tccgtgtgtc
    12661 gtcggatccg gcagcgcaac acaagtgctg gagaccggcc gggtcgtgac ggtgtcctgc
    12721 gccgaaggag acgaggggca cgtctaccaa ggggtcgtgc cattcagtcg agaagaaatc
    12781 gaacccagca aattgccgcg cccgcgcgtg cccgtgatgc tcaatgtggg caatccagat
    12841 ctggccttcc tggtcggaca actgccgagt gccggagtgg gcctgctccg catggagttc
    12901 gtcgtgtcga gttggatttc ggtgcacccg atggcgctga ttcatccgga aagggtggaa
    12961 gacccgagcg tcaccgccaa aatccgcgcg cgaacggcgg ggtacgagcg ccccaccgag
    13021 ttcttcgtcg aacgtctagc gagcggggtc gcgcagatcg cggccgcatt ctatccacgg
    13081 ccggtaatcg ttcgcatgag tgacttcaag accaacgagt attctggctt gctcggcggg
    13141 gaatacttcg aaccagtaga aaacaacccg atgatcgggt tcaggggggc ctcccgctac
    13201 tatgacgaac gctaccgcga ggggttcgcc ttggagtgcc aagcgttgct gcgagttcgg
    13261 gagcggatgg gactacgcaa cgtcaaagtc atgattccgt tctgccgaac tttggaagag
    13321 ggccgccgcg tgctcgccga gctcgatcgc aatggcctgc gacgcggcga caatggtctc
    13381 gaagtctacg tgatgtgcga gatccccaac aacgtggttc tggcggagga gttcagcgaa
    13441 ctcttcgacg gtttctcgat cggttcgaac gatctcacgc agctcatgct cggtgtggac
    13501 cgcgactccg agttgctcgc gcacgtcttc gatgaacgag atccaggggt gaagcgcatg
    13561 atcgagctcg tgatcgagcg ggcgcacaag cggggccgca aagtgggcct gtgtgggcag
    13621 gcgccgagtg atcatcccga gttcgcccgg ttcctggccg acgccgggat cgactcgatc
    13681 tcggtgacta gtgacgcctt gccgaaggtc gtacgaatct tggccaagcc ggtttagcgg
    13741 ggagtgctaa tttgcggggt gagctgccgg cagcgagctc ggcagctcac ccgtttgcag
    13801 tcgccacaac atcgagtatt ggcgaccctc ggcgatcagc gactcgtgcg tgcccgcttc
    13861 cacgatgcgg ccaccctcga gcacgtagat cacgtcggcg tgacgcacgg tcgagagccg
    13921 atgcgcgata atcaacgtgg tgcgcccgac gaccagcttg tcgagcgacc gctggatggc
    13981 cgcctccgtt tcgttgtcga cggcactggt tgcttcatcg agcaccaaga cgggaggatt
    14041 cttgaggatc gcacgggcca aggcgatgcg ctgccgctga ccaccggaga gttt
//
LOCUS       DASTKU010000423         6244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_525808, whole genome shotgun sequence.
ACCESSION   DASTKU010000423 DASTKU010000000
VERSION     DASTKU010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6244
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525808"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFQ61_14515"
     CDS             <1..377
                     /locus_tag="VFQ61_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955724.1"
                     /translation="AHPDVVTDVKAARRAPSDAPQPAATSRRVALHVEPADAHAFLAG
                     EDKGSSPVMLDVVSGQSVELEIRRDGYRSAKVIVDDSLDRRVVKLERIAPRAVYAAPP
                     PHTPRPASASQDVTIIDPWKKH"
     gene            440..1858
                     /locus_tag="VFQ61_14520"
     CDS             440..1858
                     /locus_tag="VFQ61_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET9955725.1"
                     /translation="MALSTQRLSQEVPKNVRGLCQRLHEAGHRSWIVGGCVRDELLLD
                     LPVPSGVEPRAAVSTRNDWDIATSAHPEQVMKLFSRVIPTGLQHGTVTVLMGKEQYEV
                     TTLRGETTYSDARRPDSVYFVDDIRDDLARRDFTINAIAYDVLEDRLIDPFGGIQDLV
                     ARRLKAVRDPNERFAEDGLRVLRAARFVALLELELETETARAIRPSLDSYRKVSAERV
                     RDEWLKTMKAARPSLAFEVMRQHGLLEITAPELLESVGCEQNRYHAYDVWGHALACLD
                     LSPRHPVLRVAGLLHDIGKPRTRAFSEKTKDYTFYEHERVGAEMVAPLLERLRFSNQE
                     RDHITALVRNHLICYDRAWSDAAVRRWLRRIGPELLPDLYHLAEADVRGKGRDVTSDL
                     QNIEALKAHVARILAAGAALNVRDLAISGRDLGKIGLKPGPLYGEILRRLLDEVVEQP
                     ELNRAELLLDRARQISDELAAS"
     gene            complement(1975..3912)
                     /gene="ftsH"
                     /locus_tag="VFQ61_14525"
     CDS             complement(1975..3912)
                     /gene="ftsH"
                     /locus_tag="VFQ61_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET9955726.1"
                     /translation="MKQPHKTLLLWVVLIFAFLAIWRFLSDAEGPQKQEISYSDFIAL
                     VKAPRDQRHIDEVEIKDRDYSFTVKNPGAKGPAEHGRTVGPVETPPEELLKYNVRVNY
                     KKDDGGSLVTPVLTILVPMVLVIVMFYLFMRQLQAGGGKAMSFGKSRARLLSESQNKV
                     TFADVAGIDEAKDEVEEIIAFLKDPKKFQRLGGRIPKGVLMMGPPGTGKTLLARAIAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKHAPCIIFIDEIDAVGRHRGAGLG
                     GGHDEREQTLNQLLVEMDGFESNEGVIIIAATNRPDVLDPAILRPGRFDRRITVPRPD
                     IRGREGILAVHTKKVPLASDVDLSILSAGTPGFVGADLENLVNEAALLAARQDKEAVS
                     MEDFELAKDKVLMGSERRSMVMSDLERRTAAWHEAGHTIVGKLVEGNDAVHKVSIIPR
                     GAALGVTMFLPTEDRHLMTKQQTLARIAMALGGRVAEETVFGEVTTGAQDDIKRATRL
                     ARAMVCELGMSKKLGPVAYGENEESVFLGREINNRREDYSEETAREIDNEVRDIVEAQ
                     YQVARQVIAEHRHGLDALATALLERETLDANEIEAVLAGKELPPRQRVIIPTWSEKRN
                     RDRSEKKRPASIFGTPKPAPG"
     gene            complement(4228..5166)
                     /gene="tilS"
                     /locus_tag="VFQ61_14530"
     CDS             complement(4228..5166)
                     /gene="tilS"
                     /locus_tag="VFQ61_14530"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET9955727.1"
                     /translation="MSRSHPPSVLTLVERVLRDEIVLGEQRRILVAVSGGMDSMVLLH
                     ALAHLRERLNVEVSAHGVDHGLRPEAAAELDLAESAARRWGVPFARTSVQLKPGGNLQ
                     ARARELRHRALRAASAEQRGAWIATAHHADDRAETFLLRLLRGTSPRGLAVLPAQAGV
                     LIRPLIRARRRDIQLHATRHDVPYANDPSNQDGRFLRVRVRQELVPLLERLSPGVIGH
                     LTALADELAEPQPEFPALPEPDLVLGREQRRQLSRALAEGNARIRVRVSGGRELRLNP
                     KTRSFEFVSASADTEAAAEGPSLRDRPIAGKPLDPK"
     gene            complement(5163..5951)
                     /gene="lipB"
                     /locus_tag="VFQ61_14535"
     CDS             complement(5163..5951)
                     /gene="lipB"
                     /locus_tag="VFQ61_14535"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET9955728.1"
                     /translation="MTGSLDGTPARWRPLWLGQQEYELVHELQEQLFEARKEQLIADT
                     VLFVEHPAVITLGRGARAEHVLLDETSRRAAGIALAATGRGGDVTLHAPGQLVCYPIL
                     ELPEGRRDVRRYVRDLCECMRRVAAEYGVSGGTLSDYVGLWVDADSPGDWPGEEAAVT
                     PVKIGAIGVRLSRWVTMHGFALNLTTDLSLFRSIVPCGIQQHGVGSVQTLSGVAPSVR
                     SAAELAVHHLGRIFDLPPSNLEDARLDRVRGELLRTEPTAHPSP"
     gene            complement(5948..>6244)
                     /locus_tag="VFQ61_14540"
     CDS             complement(5948..>6244)
                     /locus_tag="VFQ61_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9955729.1"
                     /translation="PVIPGVYLHHERWDGLGYPLGLRGEDIPIIARIISVADTYDAMT
                     SNRAYRRALPHELTVTEIERCAGTQFDPEVASTFVQHIERYREEQIDHGKEVPE"
BASE COUNT         1071 a         2065 c         1942 g         1166 t
ORIGIN      
        1 cggcgcatcc agacgtggtg acggacgtga aagccgcacg ccgcgcgccg tcagatgcac
       61 cgcagccggc ggccacgtcg cggcgcgtgg ccctgcacgt ggaaccggcg gacgcgcacg
      121 ctttcctcgc cggggaggac aaaggttcga gtccggtgat gctagacgta gtctctgggc
      181 agagtgtcga actcgagatt cgtcgcgatg gttatcgctc ggccaaagtg atcgtggatg
      241 attcgctcga ccgccgcgtg gtcaagctcg agcgcatcgc tccgcgcgcg gtgtacgctg
      301 cgccgccccc gcacacgccg cgtccggcgt cggctagcca agacgtcacc atcatcgatc
      361 cttggaagaa gcactgagtt aacggcagat ccgggtcgca gaatgcgcca gatgggggat
      421 ctgctaggtc tccgcctgcg tggcactctc gactcagcgt ctgtcccaag aagtccccaa
      481 gaacgtgcgt ggcctgtgcc agcgcttgca tgaggctggg catcgcagct ggatcgtggg
      541 gggctgcgtt cgcgacgagc tactgctgga tttaccggtc ccgtccggtg tcgagcctcg
      601 ggcggccgtg agcacgcgca acgattggga catcgcgacc agcgctcatc ccgagcaggt
      661 gatgaagctg ttctcgcgcg tgattccgac tggcctccag cacggcacgg tcaccgtgtt
      721 gatgggcaaa gagcagtacg aggtcaccac gctgcgcggc gagaccacgt attcggacgc
      781 acggcgaccg gacagcgtgt acttcgtcga cgacattcgt gacgatctgg ctcgccgtga
      841 tttcacgatc aacgccatcg cttacgacgt gctcgaagat cggttgatcg atccgttcgg
      901 cgggatccaa gatctcgtgg ctcgccgtct gaaagcggtg cgagacccga acgagcgctt
      961 cgccgaggac gggctacgcg tgctgcgtgc agcgcgcttc gtggcactcc tcgagctgga
     1021 gctcgagacg gagacggcgc gcgccattcg cccctcgctc gacagctatc ggaaagtcag
     1081 cgcggagcgt gtgcgcgacg aatggctcaa aaccatgaag gcagctcggc cgagcctcgc
     1141 tttcgaggtc atgcggcagc acgggctact cgaaatcacc gcgcccgagc tgctcgaatc
     1201 ggtgggctgc gaacagaacc gctatcacgc atacgatgtg tggggtcacg cgttggcgtg
     1261 tttggacctc agtccgcgcc atcccgtgct gcgcgtcgcg ggtctcttgc acgatatcgg
     1321 taagccgcgc acgcgtgctt tcagcgagaa gaccaaggac tatacgttct acgagcacga
     1381 gcgggtcggc gccgagatgg tcgcgccgct actcgaacgc ttgcgcttct ccaaccaaga
     1441 acgagatcac atcactgcgc tcgttcgcaa tcacctgatt tgctacgacc gcgcctggag
     1501 tgatgcggcc gtacggcggt ggctacgtcg cattggcccc gagttattgc ccgacctcta
     1561 ccacttggcg gaggccgacg tgcgcggcaa aggtcgcgat gtcacctcgg atctccagaa
     1621 catcgaggcc ctcaaggccc acgtcgctcg gatattggcc gccggcgccg cgctcaacgt
     1681 gcgcgatctc gccatatcgg ggcgagattt gggcaagatt ggcttgaagc caggtccact
     1741 ctacggagaa atcctgcgcc ggttgctgga tgaggtggtg gagcagcccg agctcaatcg
     1801 agccgaactg ctactcgacc gcgcccgcca aatctccgac gagctcgcgg cttcctaggc
     1861 tcgcattggc ggcagcgcca agctccgccc ccagcaaagt agaccccaaa atgcaacgac
     1921 gccggcactc atgtgagctg ccggcgtccg ttgcgcggtc tactcgttta gcggtcagcc
     1981 cggtgcgggc tttggggttc cgaaaatact ggctggacgc ttcttctcgg agcgatcccg
     2041 attgcgcttc tccgaccagg tcggaatgat gacgcgctga cgtggcggca gctccttgcc
     2101 tgccagcacg gcctcgattt cgttcgcgtc cagcgtctca cgctcgagca gcgcggtggc
     2161 gagcgcgtcg agcccgtgac ggtgctccgc gatgacctgg cgcgctacct ggtactgcgc
     2221 ttcgacgatg tcacgcacct cgttgtcgat ctcgcgcgcc gtctcttccg agtaatcctc
     2281 gcggcgattg ttgatttcgc gaccgaggaa cacgctctcc tcgttttcgc cgtacgccac
     2341 cggaccgagc ttcttgctca tgccgagttc gcacaccatg gcgcgggcta gccgagtcgc
     2401 gcgtttgatg tcgtcttgcg cgcccgtcgt cacctcgcca aacaccgtct cttctgcgac
     2461 gcgaccaccc agggccatcg cgatgcgggc cagggtttgc tgcttggtca tgagatgacg
     2521 gtcctcggtg ggcaggaaca tggtcacgcc cagcgcggct ccgcgcggga tgatggacac
     2581 cttgtgaacg gcgtcgttgc cctccacgag cttgcccacg atcgtgtgtc ccgcttcgtg
     2641 ccaagcggcc gtgcggcgct ccagatcact catgaccatc gagcgtcgct cgctgcccat
     2701 caacaccttg tccttggcga gctcgaagtc ttccatgctc accgcttcct tgtcttggcg
     2761 agcggcgagt agagcggctt cgttgaccaa gttctcgagg tcagcgccca cgaatccggg
     2821 ggttccggcg gacaggatgc tcaaatccac gtcggatgcg agcggcacct tcttggtgtg
     2881 aacggcgaga atgccttcac ggccgcggat atccgggcgg ggcaccgtaa tccgccgatc
     2941 gaagcgaccg ggccgcaaga ttgcgggatc cagtacgtcg gggcggttgg tggcggcgat
     3001 gatgatcacg ccttcgttgg actcgaagcc gtccatctcg accagaagct gattcagcgt
     3061 ctgctcgcgc tcgtcgtggc caccgccgag tccggcgccg cgatggcgtc caactgcatc
     3121 gatctcgtcg atgaagatga tgcagggcgc gtgcttcttt ccctgttcga acaggtcacg
     3181 cacgcggctc gcaccaacac ccacgaacat ctctacgaaa tcggagccgg agatgctgaa
     3241 gaacggaact cctgcttctc cagcgatagc tcgcgcgagc aacgtcttgc cggtgcccgg
     3301 agggcccatc atcagcacgc ccttggggat acgcccgccg agccgctgga acttctttgg
     3361 gtccttgagg aaggcgatga tctcttcgac ctcgtccttg gcctcgtcga tcccagccac
     3421 gtccgcgaag gtgaccttgt tctgcgactc actgagcaga cgcgcgcggc tcttgccaaa
     3481 gctcatcgct ttgccaccac ccgcctgcag ttggcgcatg aacaagtaga acatcacgat
     3541 cacgagcacc attggcacta ggatcgtgag aaccggcgtc actagcgagc cgccgtcgtc
     3601 cttcttgtag ttgacgcgaa cgttgtattt caagagctcc tcgggcggcg tctcgacggg
     3661 gccgaccgtg cggccgtgtt ccgcgggacc cttcgctccg ggattcttga cggtgaacga
     3721 gtagtcgcga tctttgatct cgacctcgtc gatgtgacgt tggtcgcgcg gtgctttcac
     3781 cagcgcgatg aagtcgctat acgaaatctc ctgtttctgt ggtccttcgg cgtcgctcaa
     3841 gaaacgccaa atcgccaaga aagcgaagat tagaacgacc cacaacagta gagttttgtg
     3901 cggctgcttc acccgaacga cctcgaggca cgagctttcg cgatcatctc gaccaaagca
     3961 tgccaccact ccccccgtgc gtcgatgggg gcacctcaaa tctcccgcat tggggctagc
     4021 tttttttgga ggctcgtttt cggcgcactt cctctccgcc aattgtggtg ttcagctgtc
     4081 cggcggcggg cggacgttcg tcacctcagc cgccgtttaa ctgtcgctga tggctcactt
     4141 tttcaggagc gcaacgtcgc ccagcgctga cgaaccggct cgtaaattgg ggcaattcac
     4201 cgcttaattt cgcgcccgag cgcgcgttca tttcgggtcg agaggcttgc ccgcgatggg
     4261 cctatcccta aggctcgggc cctccgcggc cgcttcggtg tccgcgctgg cggacacgaa
     4321 ctcgaaggat cgtgtcttcg ggttgagccg aagctcgcgt ccacccgaga cgcgcacgcg
     4381 aatgcgagcg ttgccctcgg ccagcgctcg tgacagctgg cgacgctgct cgcgtcccag
     4441 caccagatcc ggctcgggca atgctgggaa ttccggctgc ggttcagcca actcatccgc
     4501 caatgcggtg aggtggccaa tcacgcctgg cgagagccgt tcgagcaacg gcaccagctc
     4561 ctgccgcacg cgcactcgca aaaagcggcc gtcctggttg gatggatcat tcgcgtaggg
     4621 cacgtcgtgg cgagtcgcat gtagctggat gtcacgccgc cgggcgcgaa tcagtggacg
     4681 aatcaatacg cccgcttggg ccggcaacac cgcgagcccg cgcgggctcg ttccccgcaa
     4741 taggcgcagt aagaaagtct cggcccgatc atccgcgtga tgcgcggtag cgatccaggc
     4801 gcctcgttgc tccgcggacg ctgcccgcag ggcgcggtgg cgcaattcac gcgcccgcgc
     4861 ctgcaagttt ccccctggtt ttaactgaac ggatgtgcgc gcgaacggga ctccccagcg
     4921 tctcgcggcg ctctccgcca aatcgagctc ggccgcggct tcggggcgca ggccgtgatc
     4981 gacgccgtgt gcgctcactt ctacgttcag gcgttcgcgc aagtgggcga gcgcgtgcag
     5041 caacaccatc gagtccatgc cgcccgagac cgcaaccagg atccggcgct gctcgccgag
     5101 cacgatctcg tcacgtagta cgcgctcgac cagcgtcagt accgacggag ggtgagaacg
     5161 actcacggtg atggatgcgc ggtgggttcc gtccgcagca gctcgccccg aactcgatcc
     5221 aggcgagcgt cctcgaggtt gctcggaggc aaatcgaaaa tccgcccgag gtgatgcacc
     5281 gcgagctccg cggcgctccg cacgctcggc gccacgccgg acaaagtttg cacgctcccc
     5341 accccgtgct gctggatccc gcacggcacg atgcttcgga acaggctcaa gtcggtggtg
     5401 aggttcagcg cgaaaccgtg catggtgacc cagcgcgaca ggcgaacccc gatcgctccg
     5461 atctttacgg gcgtcacggc ggcctcttcg cccggccaat cgccgggcga atcagcgtct
     5521 acccagagcc ccacgtagtc agagagcgtg ccgccgctca ccccgtactc agcggccacg
     5581 cgccgcatgc actcgcacag gtcgcgcacg tagcgacgca cgtctcgtcg gccttcgggc
     5641 agctccaaga tcgggtaaca cacgagctgc cccggcgcgt gcagcgtcac atcgccccca
     5701 cgcccggtgg cggccagcgc gatccccgcc gcgcgtcggc tcgtttcatc gagcagcacg
     5761 tgctcggcgc gcgcgccgcg ccccaacgtg attacggccg ggtgctcgac gaacagcacg
     5821 gtatccgcaa tgagctgctc ttttcgggct tcgaacagct gctcttgcag ctcgtgcacg
     5881 agctcgtatt cctgctgccc caaccacaac gggcgccagc gtgcgggggt gccgtcgagc
     5941 gagcccgtca ttccggtact tccttgccgt ggtcgatctg ctcctcgcgg tagcgctcga
     6001 tatgttgtac gaaagtgctg gccacctccg gatcgaactg ggttcccgcg cagcgctcga
     6061 tctcggtgac cgtcaattcg tgcggcaacg ctcgacgata cgcgcgattc gaagtcatgg
     6121 cgtcgtaggt gtccgccacc gaaatgatgc gcgcgatgat gggaatgtcc tctccgcgca
     6181 gccccagcgg atagccgagc ccgtcccacc gctcgtggtg caagtacacg ccgggaatca
     6241 ccgg
//
LOCUS       DASTKU010000424         7434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_971969, whole genome shotgun sequence.
ACCESSION   DASTKU010000424 DASTKU010000000
VERSION     DASTKU010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7434
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971969"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFQ61_14545"
     CDS             complement(<1..838)
                     /locus_tag="VFQ61_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET9955730.1"
                     /translation="MAKIVGLPKLSPTMEEGTLSKWVKREGDDIAVDDVIAEVETDKA
                     TMEWRSFDRGVLLKILAPEGATVAAEAPIAILGQRGEDISQLLPSSVSAASAASPPSP
                     IAQAPTAPLTPRVTAPAAPPELSSPRVANAQQREPNSELRELANSPHEPSGSPQRTAS
                     EAGSARILASPSVRRLAREQSLDLRGKVGSGPGGRIIQRDLESLLQSSTRVVPTEPEV
                     TSHSPNRNIDTLGGRSEPKIVPLSAMRRTIARRLVESKQTVPHFYLTAEVRADALIAT
                     RQT"
     gene            complement(857..1885)
                     /locus_tag="VFQ61_14550"
                     /pseudo
     CDS             complement(857..1885)
                     /locus_tag="VFQ61_14550"
                     /inference="COORDINATES: protein motif:HMM:NF008854.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
     gene            complement(2030..3103)
                     /gene="pdhA"
                     /locus_tag="VFQ61_14555"
     CDS             complement(2030..3103)
                     /gene="pdhA"
                     /locus_tag="VFQ61_14555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET9955731.1"
                     /translation="MSSETPSRASSISEIAGAGESQKLAGDWGSATMSTPQWKGCYRA
                     MLLIRRIEEEAARAYSQGKIGGFLHLYIGQEAIAVGTEAALEKTDYMITTYRDHGIAL
                     ARGCSARSVMAELYGKDTGLVRGLGGSMHLFDVEHRVLGGHAIVGGHVPLAAGIAFRA
                     RYLGENAVTVCFLGDGATNIGGFHEGLSLAGLWRLPVIYVCENNRYSMGTPLTRTTPL
                     TDLTLKAPGYGMASDRFEGHDVLVVRERMLQAVERARSGGGPTLIEVMTYRFRGHSMS
                     DPGTYRTAEELDQFKRTADPCRVARDALLQAGTTEAELSELEASVESEVQDAVQFAED
                     SAPARETSIDAYVLAPARPAASG"
     gene            complement(3245..5380)
                     /locus_tag="VFQ61_14560"
     CDS             complement(3245..5380)
                     /locus_tag="VFQ61_14560"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HET9955732.1"
                     /translation="MSDPEIDLRLFQSSYFDEADEHLSTMEAALLELESNPSDAELLN
                     TIFRAAHSIKGGSGMFNLRQIQQLTHALESLLDPLRAGALAITPAIANLLFRSTDLLK
                     TLLQNAKAGSSETVDTSATLAELEKAYRAPRTPPAGGSPNVASPPQCPMKAQEPSSAL
                     QASAPHSQRFEVHFAPGAQLFESGVDPVLVVRNLCRLGENAEVRLRSESLPPLSRLNP
                     EACYLSWDIRLESQCCEAELRDVFAFVEDASTIEIRALPETERAQDLGIPAAAAPPPV
                     DEPGSPPPQQVPPPQVPAPQAAAEVDTHPREKLHEAAAATQSTLRVSTDKLDRLLNLV
                     GEIVIAQSMIVQALDHPAADTLERVRDAVVDMERNTRELQERVMSVRLVPMASVFNRF
                     PRLVRDLGLQCHKQVELRILGQETEIDKGIIEKLIDPLTHLIRNAIDHGIESPEARRA
                     AGKDETGTLTLCASHQGSSVVIEVRDDGRGLDTQRLRERGESLGLIQPGDSLSDDQIH
                     ALIFEPGFSTAERVSDISGRGVGLDVVRRNLDSANGSVVVTTERGKGTRILLKLPLTM
                     AILDGLSLRVGEQTFILPLLSITESFRPTRDQLGSIFGRSEVVRVRGEPIPLLRLYEA
                     LQVVPEKTDPLRATVVIVESGSDKVGVLVDELLGQAQFVIKSLEANFRKVECAMGATI
                     LGDGRVALILDVDSLIRRGMGSTHTALSA"
     gene            complement(5377..5670)
                     /locus_tag="VFQ61_14565"
     CDS             complement(5377..5670)
                     /locus_tag="VFQ61_14565"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9955733.1"
                     /translation="MQEQITRISLQATIDAVAALELAITARDWVALGCDLELECSALE
                     RIDAMALQVLLSLQKELATTNHTLRLSSVPSEVMQYLAYAGAAESLLGAGVKS"
     gene            complement(5763..6890)
                     /locus_tag="VFQ61_14570"
     CDS             complement(5763..6890)
                     /locus_tag="VFQ61_14570"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HET9955734.1"
                     /translation="MKAVGSSAADPARIRVLVVDDSALMRQLLRQLIENDPELCVVDT
                     ASDPLQAWNKIQSRTPDVLTLDVEMPKMDGLTFLDKLMRAQPLPVVMVSSLTEQGCDT
                     TLRALELGAVDYVCKPQMDLAQGMHELAHELRQKLKVAARARVQARLRSHSAPAPRKP
                     SVSPRGLEGARADARKVPGAKLDPSRTSPRVVAIGASTGGTEALKSVLTALPPDAPGI
                     VIVQHMPENFTRQFANRLNGLCRIEVREAADQDRITPGLALIAPGSHHMQVEKAVGGF
                     RVRLQAGPPIDHHRPSVDVLFESCAKTIGAQALGVILTGMGSDGARGLAAMRRSGAFT
                     LGQDEQTCVVYGMPKEALLRGGVARQAPLGQIASLIVAHTR"
     gene            complement(6887..>7434)
                     /locus_tag="VFQ61_14575"
     CDS             complement(6887..>7434)
                     /locus_tag="VFQ61_14575"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HET9955735.1"
                     /translation="RFNISSGEWHASATPARISTVLGTCVAVCLYDPVKQVGGMNHFM
                     LPQATGEEGPLAARYGVHAMELLVNTLMELGADRAGLRAKVFGAAHVIRGMESGEAVA
                     RKNAHFVRTFLEREAIPVEAEFLGGNQALVVQFFTHTGKARVKPLSVIPQQLIERDRS
                     GSSTMSAHEPVLFGAEQDVSS"
BASE COUNT         1340 a         2364 c         2319 g         1411 t
ORIGIN      
        1 atgtttgcct cgtcgcgatc aaagcgtcgg cccgcacttc cgccgtgaga tagaaatggg
       61 gtaccgtctg cttggactct acgagccggc gcgcgattgt gcgccgcatg gccgagagcg
      121 gcacgatctt cggttccgag cgcccaccga gagtatcgat attgcggttg gggctgtgac
      181 tcgtgacttc gggttcggtc ggtaccactc gagtcgatga ttgcagcaag ctctccaagt
      241 ctcgttgaat gatgcgcccg cccggcccgc tgcccacttt gccgcgcaaa tccagcgatt
      301 gttctcgtgc caagcgtctg accgaaggag acgccaagat ccgtgcggag cccgcttcgc
      361 ttgccgtacg ttgtggagag ccactcggct cgtgcggcga attggctagc tcacgcagct
      421 cgctgtttgg ctcgcgctgc tgcgcgttgg ccactcgcgg cgacgagagt tcgggaggcg
      481 cggccggtgc ggtgactcga ggcgtaaggg gagccgtggg tgcctgggct atcggcgaag
      541 ggggggacgc cgcggatgcg gcggatacgc tggagggaag tagctgacta atgtcctctc
      601 cacgctggcc cagaatggcg atgggtgctt cggcggcgac ggttgctcct tctggtgcga
      661 ggatcttgag tagaaccccg cggtcgaacg aacgccactc catcgtagcc ttgtcggtct
      721 ccacttcggc gatgacgtcg tctacggcga tgtcgtcccc ttcgcgcttg acccacttgg
      781 agagcgtgcc ctcctccatg gtgggtgaca acttgggaag tccaacgatc ttggccattg
      841 ttgatccagc ttgaggtcag tcgagataga gcacttgacg cagcgcttgc cggacgcgct
      901 cgggtgtaac gatcacctga ttttccagat tgagagcgta aggcatcggc acatccaggt
      961 tgctgacccg caacacgggg gcatccaagt agtcgaaggc tactcgctga atacggtcca
     1021 cgagctccgc gccgggccca ccatagggcc agtgctcgtg gacaatcacg cagcgattgg
     1081 tcttccgcac actggcaacg atggtttcgg tgtcgagtgg cctcaagctg cgcggatcga
     1141 tcagctccat cgagattccc tcacgctcca gctcgtcggc gagcttggcg agcatcggga
     1201 gcggtcgacc gaagctcacg acggtgacgt gccttccttc acgcacgata cgcgcagaac
     1261 cgaacgggat cagctcgtct cgaccggcct cgggaacttc gcccttgagc ccatacagcg
     1321 tctcgccctc tagaaagacg accggattgt catcccgtat tgctgtcttc aggagcccct
     1381 tcgcgtcccg cggactcgag gggtagacta cgtgtagccc ggggacatta caatagaagg
     1441 cctctacggc gtggctgtgt tgcgcggcca cctggcgagc agaggcgttc ggtccgcgaa
     1501 acacgattgg gacgcgaaac tgccccgcac tcatctgatg aattttcgcg gcgttgttta
     1561 cgatctgatc gaaagcaacc agggaaaagt tccaagtcat gaactccacg atgggccgca
     1621 gcccgaccat tgcggcccct acggccaacc cggcgaagcc cgcttcggcg atcggtgcgt
     1681 ccaccacccg tcgttctccg aagcgctcga gcaggccttg agtcaccttg tacgcgccct
     1741 gatagtatcc aacctcttcg ccaatcagaa acacgcgctc gtctcgttcc atttcctcga
     1801 gcatcgcctc gcgcagcgcc tctcgataac gtatctcacg catggtcttt gacttcctcg
     1861 ggaacggagt ggcttggatc gaactatccg ggcgtggtcg cttcgtcgct ggtgcagagt
     1921 catttcgcta acagggcgcg tccctccaga acgaagccct tcggagcgga ctggctttcg
     1981 agagaatcgg agtcgaatcc gggaacgcca gggcgccgaa tggctggact caccccgagg
     2041 cggccggcct ggcaggggcg agcacgtatg cgtcgatgct cgtctcgcgc gccggtgcgc
     2101 tgtcttccgc gaactgcacc gcatcttgga cttcggactc cacgctcgct tcgagctccg
     2161 agagctccgc ctcggtggtc ccggcctgca gcaaagcgtc gcgggccacg cgacacggat
     2221 ctgccgtgcg cttgaattgg tcgagctcct ccgcggtacg gtaggtgcca ggatccgaca
     2281 tcgaatgccc gcgaaagcga taggtcatca cctcgatgag cgtcggccca ccgccggacc
     2341 gggcgcgttc gacggcctgg agcatccgct ctctgaccac gagtacatcg tgcccttcga
     2401 aacggtcact cgccattcca tagcccggcg ctttgagcgt gagatccgtg agcggagtgg
     2461 tgcgggtgag cggagtgccc atcgaatagc ggttgttctc acaaacatag atgaccggca
     2521 gtctccacaa tccggcaagg gacaggcctt cgtgaaaacc accaatgttc gtggcgccat
     2581 cgcccaagaa gcagaccgtc accgcgttct caccgaggta gcgagcacgg aacgcgatgc
     2641 ctgcggcgag cggcacatgg ccacctacga tagcgtgtcc gccaagcacg cggtgctcca
     2701 cgtcgaacaa atgcatggag ccgccgagcc cccgcacgag gcccgtatct ttgccataaa
     2761 gctccgccat cacgctgcga gccgagcagc cccgagcgag tgcaatccca tgatcgcgat
     2821 atgtcgtaat catgtagtcg gtcttctcca acgccgcttc ggtgccgacc gcgatcgcct
     2881 cttgtccaat gtacaagtgc agaaatccgc cgatcttgcc ctgcgaataa gcccgggccg
     2941 cctcctcctc gatgcgccga atcagcagca tggcgcgata gcaccccttc cactgcggag
     3001 tcgacatggt ggcggagccc caatcgccgg cgagcttctg gctttcgccg gctccagcaa
     3061 tctcggagat ggacgaagct cggctcggcg tttcgctgct catctatcct cactcggcac
     3121 gatagccgag attgcggtcg atgacagctt atatcgagag ggcgtttata gacatagagt
     3181 cgtgaggcgg ggagccgcgc ggcgcattgc ctcgtgcgtg gaatctctca ctcgacgagg
     3241 ccgctcacgc ggacagcgcg gtatgagtgc tgcccatgcc gcgacggatc aagctatcga
     3301 cgtcgaggat taacgcaact ctgccatcac ccaggatggt agcccccatc gcgcactcca
     3361 ctttgcgaaa gttcgcctcc aggctcttga ttacgaattg cgcctgcccc agcaactcat
     3421 cgaccaagac tcctactttg tccgatccgg actcgacgat tacgaccgtc gcgcgcagag
     3481 ggtcggtctt ctccggcacc acctgtaacg cttcgtacaa gcggagcagc ggaatgggct
     3541 cgccgcgcac gcgcacgact tcactgcgtc cgaaaatcga gccaagctgg tcgcgggtag
     3601 ggcgaaaaga ctcggtaatg gagagcaacg gaaggatgaa cgtctgctcg ccgactcgca
     3661 gcgagagccc gtcgaggatc gccatcgtca gcggtagctt gagcaggatg cgcgtgcctt
     3721 tcccgcgctc cgtggtcacg acgacggagc cgttggcgct gtccaggttg cggcgcacca
     3781 cgtccaaccc gacccctctt ccagagatgt cgctcactcg ctccgcggtc gagaagcctg
     3841 gctcgaaaat tagggcgtgg atttgatcgt ccgacaagct gtcaccgggc tgaatcagtc
     3901 ccagtgattc gcctctttca cgcagtcgtt gcgtgtcgag gcctcgtccg tcgtcgcgca
     3961 cttcgatcac gacgctgctg ccctggtgag acgcgcacag agtgagagtc cccgtctcgt
     4021 ccttgccggc ggcgcgccgc gcctcggggg attcgatgcc gtgatcgatt gcgttccgaa
     4081 tcaagtgggt caagggatcg atcagctttt cgatgatgcc tttgtcgatc tccgtctctt
     4141 ggccgagaat acgtagctcg acctgcttgt gacattgcag gccgaggtcg cgcacgagcc
     4201 gagggaagcg gttgaataca ctcgccatcg gtaccaggcg cacggacatg acgcgctctt
     4261 gaagctcgcg ggtgttgcgc tccatgtcca ccaccgcgtc gcgcacccgc tccagcgtgt
     4321 ccgccgcggg gtgatcgagc gcctgcacga tcatggattg cgcgatcacg atttcgccta
     4381 ccaagttgag taggcggtcg agcttgtccg tcgatacgcg caaggtcgat tgggttgcgg
     4441 cggcggcctc atggagcttc tcgcgaggat gggtgtccac ctcagccgct gcttgtggcg
     4501 ctggcacctg cggagggggc acctgttggg gaggcggtga acctggctca tccactggtg
     4561 gcggggcggc ggctgcggga atcccgaggt cctgggcgcg ctccgtttcg ggtaacgcac
     4621 gaatctcgat cgtgctcgcg tcctccacga acgcgaatac atctcgcagc tcggcctcac
     4681 aacactggct ctcgagacgg atgtcccagc tcaggtagca cgcctctgga ttcaaacggg
     4741 agaggggagg cagggactca ctgcgtaggc gaacttccgc gttctcgccg aggcgacaca
     4801 gatttcgcac gaccaagacc ggatctacgc ccgactcgaa cagctgagcg ccaggggcaa
     4861 agtgcacctc gaagcgctgc gagtgcgggg cgctcgcctg aagcgcagaa ctcggctcct
     4921 gcgctttcat cgggcattgc ggcgggcttg cgacgttcgg tgacccgccc gcgggaggag
     4981 tacgaggcgc gcgataagcc ttttcgagct ccgccaaggt tgcgctggta tccaccgtct
     5041 cgctactacc ggccttggcg ttttgcagca gggtcttgag gagatcggtg gaacgaaaga
     5101 ggaggttggc gatggccggg gtaatcgcga gcgcgcctgc ccgcagcgga tcgagcagac
     5161 tctccagggc gtgggtaagc tgctggatct ggcgcagatt gaacatcccg ctgccaccct
     5221 tgatcgagtg cgccgcgcgg aaaatggtgt tcaagagctc ggcgtcggag ggattgctct
     5281 cgagctcgag cagcgccgcc tccatggtcg acaaatgctc gtcggcttcg tcgaagtagc
     5341 tgctctgaaa caagcgtagg tcgatctccg ggtcactcat gatttcaccc ccgcgccgag
     5401 taggctctcc gccgctcctg cataagccaa gtactgcatg acctcgctgg gtaccgagct
     5461 gagccgcaga gtgtggttcg tggtcgcgag ctccttctgg agcgacagca agacctggag
     5521 ggccatggca tcgatgcgct cgagcgcgct gcattcgagt tcgagatcgc agcccaacgc
     5581 gacccaatca cgagcggtga tggccaattc cagcgccgcg accgcgtcga tcgtggcctg
     5641 cagagatatc cttgtaatct gctcttgcac gcttccctac cttctatgtt acggccgcac
     5701 cgagccccga attaacctgc ggctcggtgc caccggcggt gcacgagaga cgcggcgcgc
     5761 cgtcagcgcg tgtgggcaac gatcaggctc gcgatctgtc ccaagggagc ctgacgggct
     5821 acgccacctc gcagcagcgc ctccttgggc atgccgtaaa cgacgcaggt ttgttcatct
     5881 tggccgagcg taaaggctcc gctgcggcgc atcgccgcga gcccgcgcgc gccgtcactt
     5941 cccataccgg tcaggatcac accgagcgcc tgcgccccga ttgtcttggc gcaggactcg
     6001 aagagaacgt ccaccgaagg gcggtggtgg tcgatcggtg gtccagcttg gagacgaacg
     6061 cggaatccgc ccaccgcttt ctcgacctgc atgtggtggc tgcccggcgc aatcagggcc
     6121 aagccgggcg tgatgcggtc ttgatcggcg gcttcccgca cctcgatccg gcacagcccg
     6181 ttcaagcgat tggcgaattg ccgggtgaag ttttcgggca tgtgctgcac gatcacgatg
     6241 cccggtgcat ccggaggcag cgccgtgaga acggacttga gcgcttcggt accccccgtc
     6301 gacgcgccga tcgcgaccac ccggggcgac gttctgctgg gatctagctt cgccccggga
     6361 accttgcgtg cgtcggcgcg agcgccttcg aggccccgag ggctgaccga aggcttgcgc
     6421 ggagctggcg ccgagtgcga gcgaagccgg gcttgtacgc gggcgcgcgc ggcgaccttg
     6481 agcttctgcc tcaattcgtg ggctagctcg tgcatgccct gcgcgagatc catctgcggc
     6541 ttgcagacgt aatctaccgc cccgagctcc aacgcgcgca aggtggtatc acagccttgc
     6601 tcggtcaggg acgagaccat cacgaccggc aacggctgcg cacgcatcaa cttatccagg
     6661 aacgtgaggc cgtccatttt cggcatctcg acgtctaggg tcaagacgtc gggagtgcgg
     6721 gactggatct tgttccacgc ttgcagtgga tcggatgccg tatccaccac gcacagctcc
     6781 ggatcgtttt cgatcaactg gcggagcagc tggcgcatca aggccgagtc atcgacgacg
     6841 agcacgcgaa tacgcgccgg atccgccgcg ctagaaccaa ctgctttcat gacgacacat
     6901 cctgttccgc gccgaacaac accggctcgt gtgcgctcat cgtcgaagaa cctgaacgat
     6961 cgcgctcaat caactgctgt gggattacgc tgagcggttt gacccgagcc ttgcccgtgt
     7021 gcgtgaagaa ctgcacgacc aaagcctgat tgccgccgag aaactcggcc tcgacgggaa
     7081 tcgcttctcg ctccaagaac gttcgcacga agtgcgcatt ttttcgggcg acggcctcgc
     7141 ctgactccat cccgcggatg acgtgggcgg ccccgaatac cttggctcgc aagcccgcgc
     7201 ggtcggcccc gagctccatc agggtgttga ccaggagctc catcgcgtgc acgccgtaac
     7261 gcgcggcgag cggaccctct tcgccggtcg cttgagggag catgaagtgg ttcatgccgc
     7321 ccacctgttt gaccggatcg tacaagcaga ccgcgacgca cgtgcccaag acggtgctga
     7381 tccgagcggg cgtcgcgctg gcatgccact cgccgctcga gatattgaac cggt
//
LOCUS       DASTKU010000425        10456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1865627, whole genome shotgun sequence.
ACCESSION   DASTKU010000425 DASTKU010000000
VERSION     DASTKU010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10456
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865627"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(499..897)
                     /locus_tag="VFQ61_14580"
     CDS             complement(499..897)
                     /locus_tag="VFQ61_14580"
                     /inference="COORDINATES: protein motif:HMM:NF022443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HET9955736.1"
                     /translation="MKHGRERLQTLGWIGLVIVLGGVSRLPAMPEFSHLYLGDVLWGS
                     LFYLITRFLLPTLSARSAWLMATLAAELVEFGELSQAPTLVSIRETRLGGLLLGHSFS
                     WSDVFCVALGSSLAFALQKPRSRSARLASP"
     gene            complement(1065..2363)
                     /locus_tag="VFQ61_14585"
     CDS             complement(1065..2363)
                     /locus_tag="VFQ61_14585"
                     /inference="COORDINATES: protein motif:HMM:NF015187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HET9955737.1"
                     /translation="MKTLRLSLGFVCTLFVACSAENTGLDPGASAGRSSAPPSAGGTS
                     GGNGAGAAPGAGSTSSSGVGGANMSTAGTPSASMGGVSASQGGRSTGNPIGGAQSAGA
                     SSIGSGGQANAGSAGGGASDHGGASNAGGSAQGEAGTAGKAAGGSSSGGGANEPGNGG
                     RAGASSGGTSSSNGGAPRGGASSSGGALNSGGATTNGGAANGGSSGSCVAWPSAKGEQ
                     SVSTTIKVSGNYDGGLKRFTGSGALGTSGQSEDQGPLFELESGATLSNVIIGSPAADG
                     VHCKGTCTLSNVWWEDVGEDAATLKGSSSSQVMTIDCGGAKKADDKVFQHNGPGTMII
                     RNFSVDDFGKLYRSCGNCKTQYERHSIFENVTAKGGSVLAGVNTNYGDSAKFSRVTIT
                     GKISICDRFTGNSTGDEPTKTGSGADGKYCIYQPSDITQK"
     gene            2792..4576
                     /locus_tag="VFQ61_14590"
     CDS             2792..4576
                     /locus_tag="VFQ61_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955738.1"
                     /translation="MIRYPLIALIAALVSVTGCSSNGDSTPIFDPGGGSSGARTGAGA
                     GALSGAGGSNTGATSAGSHFGGLGGTTTVPIASSGGLVNGGTTGTWLRNSGGTNGPTP
                     SGGTTSSPSGGASNASTGGIAQAGTGGRSSNSSGGTSNPANGGTSGSISGESGRASTG
                     GAGGSGLGGSSPTVPGELDLIAAPNGKDSAPGTIDQPTTLSAALTRVTAGHSIFLRAG
                     TYSSAVQLTIARDNSGAKDKPKRLAAYGSERPVLDFSTQTYGSGSNPRGLQINGNYWY
                     VKGLEVKGAADNGIYVAGSNNVIEACVTHGNRDTGLQIGRASSDQTNPADWPSNNLIL
                     NCESYDNYDAPPGSGENADGFAAKLTVGNGNVFRGCVSHHNIDDGWDLYTKTETGPIG
                     PVTIDRCVAYSNGTLTDGTSNDNGDRNGFKLGGDDIAVPHVVTRSVAFKNGKNGFTWN
                     SNPGAIRLSNNLAFDNAEGNFKFGDNSTNTAAVFTNNVSLWTSTGSTQSDKAMGTDVA
                     NSNVWWDKSKSSVNAKGLVATSADFASSLTTPKLSRAADGSLDFSTFSLASGSDLANA
                     GVVPEGSLPFEPADYYLGAPDLGAEETR"
     gene            4854..6566
                     /locus_tag="VFQ61_14595"
     CDS             4854..6566
                     /locus_tag="VFQ61_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955739.1"
                     /translation="MAEAVVTAGATGGTASASGGGSSGGGAPVAGASAAPYTSGGREP
                     GRACATASVWLGDLTLNQQDDVERHRGLARVAGSLRISGDVRDLAPLACLASIERDLS
                     LFELTELESLNGLETLRSIGRDLELRDNPRLDSLNALSSLKSLGGALYLSDNPRLAAL
                     TGLGGISGLRGLGIYKNDALNSLQGLPVIQSLEAGLTVSNNANLTSLAALTGLGSVGT
                     DLTLSDNPRLERLTGLDGLTQVGGDLQLGNNAQLHDLGALAKLRDIRGALAVFGNPRL
                     KQLDGLTGVGEVLTLTVSENESLLSLTGLSSLRRVVSRVYVHDNAALTNLDGLSGVEG
                     ASDLEISDNPALVQIDGMSGLRALSGGADVSRNAALASLDGLRNLVEVGGDLSIEGNP
                     RLSNVDGLSSLMRVGSLHLKDNTALRSLLGLSRLTYADSIVLEGLPVQDLAGFGGLSR
                     LNGSLTLTRDPNLTDLSGLGKLASVAGNVVIAADAHLVDLQGLPNLARIGGDFVVESN
                     PKLERLESLWSLAWIGGAFTVRDNPSLPACEPEQLRNQIGASNIQGPVLMSGNNGQGR
                     CGLL"
     gene            complement(6590..7711)
                     /locus_tag="VFQ61_14600"
     CDS             complement(6590..7711)
                     /locus_tag="VFQ61_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955740.1"
                     /translation="MSPRWRHAPSAPVKRCIGQHFLFQASEACLLSAVLLLAHCSVDA
                     DHVFKGSGGSSSTSGAGGKDSAGGTSGGGGAAGDTQAAGEAGNPQECRINAQCNGGQI
                     CLNRRCVDLGCYDGIQDQDETGTDCGGSMCEPCAAATGGKSAGSGGTASAPQGGTSSS
                     RGGTSSTNGGAGGAGGAGGTGGGSAVAECTPYKGALKVASKPFVNGYGVSGTWMGHAY
                     TYSYGAGVVVTPIPTETMSCFVGNQLCAAGSIAAYDSSGAGIGWSIAQARDDEAKPVS
                     VASVKLQLAGATAGMRVSLQPGDQTEGYCYTLSAADATTANAGLLNIPISSFQKFCYS
                     PEEAIPYTGQPVLAIDIFVLGDNAAAKQFDFCIVDVQGS"
     gene            8505..9524
                     /locus_tag="VFQ61_14605"
     CDS             8505..9524
                     /locus_tag="VFQ61_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955741.1"
                     /translation="MRQERVAALDRKYAAALGRARRLQQEGKVEEARKAFITAAGEGE
                     DVYHRAIGELAFLEFKHAMGSDQEVEDTLWVAAASEDPVVAGQAFFNLAALYARQGKA
                     EPERAALARSLARNPSVAAEKKLGRRSRCAVEVGSGPDADAKVVTGWAGVCQALDRCD
                     AKQPLDEAHARARSCVTTTARAGEPEEVHGCTEEPPWDSTYNYSLYRYWHGIIQPLPS
                     HQYWVYRAGEGGWSSVCSGDQRSTASLVQGVLWLHYTSTSSQVGRDRMAPQAGGVCWD
                     APEDNTHTFYSKTTGKKLATIRVLSQDEVQVEVSPDGRSVRLGGAHCSGRVPLDGSNR
                     LLADR"
     gene            9746..>10456
                     /locus_tag="VFQ61_14610"
     CDS             9746..>10456
                     /locus_tag="VFQ61_14610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9955742.1"
                     /translation="MQDDTLRYCVACRETLPGEARFCPACGHDSTLPECLPSPAVARQ
                     QVGRRDVDPEPDLPPMRLAPGTRLSVYRIESVLGEGGMGVVYRAYDEALSRPVAIKCL
                     HTNLAGDPEIRRRFEREARLLRTYSHPNVVSVFDFVEYEYLLAIVMELVEGPTLIRYL
                     QSWRGRPPLGEVRDLFGGVLDAMEAGHQRGIIHRDLKPENVLLMRVGAGLRAKVVDFG
                     IAKILEGTTYTVSGMFLGT"
BASE COUNT         1893 a         3175 c         3394 g         1994 t
ORIGIN      
        1 taacggcgcg cgccgcgact cggaacaata tcaccttggt aagtgattac aagactcatc
       61 gccagtcacg tgccttaccg aggcatcgag aatccacgac cggtgtggtt tggctggttc
      121 tctcggttta tggagcaatc ggctcgcaaa cttggcggta agagcgctcg cgctagcgaa
      181 cgggcggcta gcgcgagtgg ttcgtggcgc tcgcagctcg cactgacgag tgtttgcaga
      241 ctcgaatgcc gactagcatg aagcgtgttc ggatcgtgta aacgatgttt cggttcacat
      301 tagtgactga gccgaatctt acgaagaggc aggatttcga tgatctcttg cgacgcagga
      361 tgctcgtgta tgtggctgtg ttcgggagca ctcctcgagc ttcggacctg gcgccggagt
      421 cagtgagcta gtgtctcccc gagccagtgt ctccccgagc cagtgtctcc ccgagctagt
      481 gtctccccga gctaggtttc acgggctcgc gaggcgcgca cttctggacc ttggcttctg
      541 cagcgcgaaa gccaggctcg agccgagcgc tacgcagaag acgtcactcc acgagaagga
      601 atggcccagg agcaggccgc ccaggcgcgt ctcgcggatg cttaccaggg ttggagcttg
      661 ggagagctcg ccaaactcga ctagctcggc cgcgagtgtt gccatgagcc aagcggaacg
      721 cgcgcttagg gtgggcaaca ggaaccgcgt gatgagatag aaaaggctcc cccacaacac
      781 gtcacccaaa tacaagtgcg aaaactcggg catcgctggc agccgcgata cgccaccgag
      841 aacgatcacc aacccgatcc aaccaagcgt ctggagtcgc tcgcgtccgt gcttcattgg
      901 gtccagagaa gcacaactgc ggcggtgacg cctgcgccgg ttcgaggagc attcgtgcgt
      961 tctcgatgaa gaatctgctc ggtctctcct aaaaggcggc ccccctcgtc agatgagatg
     1021 cggcttcggc gtaacgacgc ggcttcgccg tacctggagc caggctactt ttgggtgatg
     1081 tcgctaggct gatagatgca gtacttgcca tccgcgccgc tgccggtctt ggtgggctcg
     1141 tctccggtgg agttgcccgt gaaccgatcg cagatggaga tcttgcccgt gatggtgacg
     1201 cgtgagaact tggcggagtc accgtagttg gtgttcacgc cggcgagtac gctgccgccc
     1261 ttggcggtga cattctcgaa gatcgaatgt ctttcatatt gagtcttgca gttgccgcac
     1321 gagcgataga gcttaccgaa gtcgtccacg ctgaagttgc ggatgatcat cgtgccaggg
     1381 ccattgtgct ggaatacctt gtcatcggcc ttcttcgccc cgccgcaatc gatggtcatg
     1441 acttgtgagc tgctcgagcc cttgagcgtc gcggcgtcct cgccgacgtc ctcccaccat
     1501 acgttcgaga gggtgcaggt gcctttgcaa tgaacgccgt ccgccgccgg ggagccaatg
     1561 atgacgttgg atagcgtggc tcccgactcg agttcgaaga gagggccttg gtcttcggat
     1621 tggccactcg tgccgagcgc gcccgaaccc gtgaaacgct tcaaaccccc gtcgtagttt
     1681 ccagaaactt tgatggtcgt ggagacagac tgctccccct ttgcgctggg ccacgccaca
     1741 caacttccac tggagcctcc gttggccgcg ccgccgttgg tcgtagcacc accgctgttc
     1801 agcgcgcctc cggatgaact cgcgccgccg cgcggagcgc caccgttact gctcgaagtc
     1861 cctccgctcg aagctccggc gcggccgcca ttgcctggtt cgttcgcgcc gcctccggag
     1921 gaggatccac cagcagcttt gcctgctgta cctgcttcgc cctgtgcgct tcccccggcg
     1981 ttgctggcac caccatggtc gctggcgccg ccgccggcgg agccggcatt cgcctgcccg
     2041 cccgagccga tcgaactcgc tcctgccgat tgcgctccgc cgatcgggtt ccccgtgctg
     2101 cggccgcctt ggcttgcgga aacgccgccc atcgacgcgc tgggtgtgcc ggctgtactc
     2161 atgtttgctc cgccaacccc ggagctgctc gtgctccccg cgcccggggc tgcccctgca
     2221 ccgttacctc cactcgtccc gccagcactc ggcggtgcgc tcgaacggcc tgccgacgct
     2281 ccgggatcga ggccggtgtt ttcagcgcta caggccacga atagagtgca aacaaagcct
     2341 agggagaggc gcagcgtctt catgacaagc tctccagtgt ccgcgacgcc ccgccgcggg
     2401 tcacgcaaag gtgcagctca ggcttgctcg aacctcgtat gcgttggtga aaaatctcag
     2461 gcacggcgca gcgccaaaag taaggagacc gctgatttca gaaaagcagc ggagaaggca
     2521 aaaagctgac ccactgagct gctcccgtac cacttgcaac tcatctgctg cgttcaagcc
     2581 gctgatagac ccgtccccgc tgagcgagtc ggcggcaacc ggttctcctg ctgggacggc
     2641 taaacgaacc tgcgtcaccg attctttgca ccggctctcc tccattcgtt cactcgggcg
     2701 aagtgaaccg agaatcttct gtggcggctg gtcgagagca cgcgagtctt ctgcgaggcg
     2761 caccgagctc aaattcgaga tgagaacacc catgatccga tatcccctga tcgcattgat
     2821 tgctgctcta gtgtcggtta caggttgttc gagcaacggc gattcaaccc caattttcga
     2881 tccgggcggc gggtcttccg gcgcccgaac gggtgcgggt gcgggtgcgc tcagcggagc
     2941 gggtggaagc aacacgggcg ccactagtgc gggctcgcac ttcggtggtc ttggcggcac
     3001 gacgaccgtt ccgatcgctt cgagtggagg cttggtgaac ggtggcacga cgggcacgtg
     3061 gctcagaaat tctggcggca cgaacgggcc cactccttcg ggcggaacga cgagcagccc
     3121 atcgggcggc gcaagcaacg cgagtacggg tggcatcgcg caggcgggca cgggcggtcg
     3181 cagctctaac tcgagcggcg gcacgtcgaa cccagccaac ggcggcactt cggggtcgat
     3241 cagcggagaa agcggaagag ccagtacggg tggggcgggt gggtccggcc tgggcgggtc
     3301 gagccctacg gtgcccggcg agctcgattt gattgcagcc cccaacggca aggacagcgc
     3361 gcccggcacc atcgaccagc cgacgacctt gagtgccgcg ctgacgcgcg tgaccgccgg
     3421 tcacagcatc ttcttgcgtg caggcacgta ttcttcggcg gtgcagctca cgattgctcg
     3481 cgacaacagt ggcgccaagg acaagcccaa gcgtttggcc gcttatggga gtgaacgtcc
     3541 cgtgctcgac ttttcgacgc aaacctacgg ttctggctcg aacccgcgtg gactccagat
     3601 caacgggaac tactggtacg tgaaaggtct ggaagtgaag ggggcggcgg acaatggcat
     3661 ttacgtggcg ggcagcaaca acgtcatcga agcgtgtgtc acccacggta accgagacac
     3721 cggccttcaa attgggcgag ccagctctga ccaaaccaac cccgcggact ggccgtcgaa
     3781 caacctgatc ctgaattgcg aatcttacga taactacgat gctccgcccg gaagcggtga
     3841 aaacgccgat ggttttgcgg cgaaactcac ggttggcaac ggcaatgtgt tccggggctg
     3901 cgtctcgcat cacaacatcg acgacggctg ggatctctac acgaagacgg agacgggtcc
     3961 gattgggccc gttaccatcg atcgctgcgt cgcctactcg aacggtacgt tgaccgacgg
     4021 aaccagcaac gacaacggtg atcgcaatgg cttcaagctg ggcggagacg acatcgcggt
     4081 gccgcacgtc gtgacccgct ccgtcgcgtt caagaatggc aagaatgggt tcacttggaa
     4141 cagcaacccc ggcgccattc ggctcagcaa caatctcgca ttcgacaacg ccgagggcaa
     4201 tttcaagttc ggggacaaca gcaccaacac cgccgcggtg ttcacgaaca atgtctccct
     4261 gtggacgtcc accggcagca cgcagagtga caaagccatg ggcaccgacg tggcgaacag
     4321 caacgtgtgg tgggacaaat cgaagtccag cgtgaacgcg aaggggttgg tggccaccag
     4381 cgcggacttt gcgagctcgc tcacgacccc caagctctcc cgcgccgcgg acgggagcct
     4441 ggacttctct acgttctcgc tcgcttcggg cagtgatctc gctaacgccg gggtcgtgcc
     4501 cgaaggtagt ttgccgttcg agcccgcgga ctactacctc ggcgcgccag acttgggagc
     4561 ggaggaaacg cgctgagggg attggctccg gctcgccgct cgttcggcgt ccgtcgagac
     4621 cgatggtgtc gactcgcaaa aacaatccgg tggttcggta actggtcgtg gctttcgccc
     4681 actggcttga acttcgcgcc tgttagtcgg tgcgaaacca agctcgagac aagagagggc
     4741 ccaatttcga gttggcgccg gtccgcgctc gatcttagat cgctcgcgtg ttggtcggtc
     4801 gctggcttgc tcttgctctg gttctatcct gtggtagccg aacgggcccc gtcttggccg
     4861 aagccgtggt gacggcaggg gcgacgggtg gcacggccag cgcctccggc gggggctcat
     4921 ccggcggcgg agccccggtt gcgggcgcat ccgcagctcc ctataccagc ggcggtcgtg
     4981 agccaggacg ggcgtgtgcg acggcttcgg tgtggctcgg ggatctgacc ttgaaccagc
     5041 aggacgacgt ggagcgtcac cggggcttgg ctcgagttgc gggcagcctc cggatatcgg
     5101 gcgacgttcg cgatctcgct ccgcttgcct gtctcgcatc gatcgagcgg gacctctcac
     5161 tgttcgagct caccgagctc gagagcctga acggactcga gacactacga agcatcgggc
     5221 gtgatctcga gttgagagat aaccctcgcc tcgatagcct aaacgccttg agcagcctga
     5281 aaagcctcgg tggcgccctg tacctgtcgg acaaccctcg gctcgcagcg ctgactgggc
     5341 tgggaggtat cagcggtctg cgtggactcg gcatctacaa gaacgacgcc ttgaacagct
     5401 tgcaggggct tccggtcatt cagagtctcg aggcggggct caccgtatcc aataacgcca
     5461 acttgacgag cctcgcggcg cttaccggcc ttggtagcgt ggggaccgac ctgactttga
     5521 gtgacaatcc gcggctcgag cgcttgaccg ggctcgatgg cctcacgcaa gtcgggggcg
     5581 atctccagct cggcaacaac gcgcagctcc atgatctagg cgcgctggcg aagttgcggg
     5641 acatccgcgg agccttggct gtcttcggca accccaggct gaagcagctg gatgggctta
     5701 cgggcgtcgg cgaagtcctg accctgacgg tctccgagaa cgagtcactt ctaagcttga
     5761 cggggttgag ctctctacgt cgtgtggtca gccgggttta cgtgcatgac aatgctgcgt
     5821 tgacgaacct ggatggcttg agcggagtgg agggcgcttc ggatctagag atttccgaca
     5881 atccggcgct cgtccaaatc gacggtatgt cgggccttcg tgcgctctcg ggcggggccg
     5941 acgtgtcacg caatgccgcg ctcgcgagcc tggatgggct gcgcaatctc gtggaagtgg
     6001 gcggggatct ctcgattgaa ggtaacccgc gtctctcgaa cgtggacggg ctgagcagct
     6061 tgatgcgtgt cggcagcttg catttgaaag acaatactgc gctcaggagc ctgcttggtc
     6121 tctcccggct gacctacgct gacagcatcg tgctcgaagg cctccccgtg caagatctcg
     6181 cagggttcgg agggctgagc cgtttgaacg gcagcctgac gctcacacga gacccgaatc
     6241 tcacggatct gagtggtttg ggcaaattgg cgagcgtagc tgggaacgtg gtgattgcgg
     6301 cggacgcgca cttggtggac ttgcagggac ttccgaatct ggctcgaatc gggggagact
     6361 tcgtggtcga gtccaacccg aagctcgagc gcctcgagtc actctggagc ctcgcttgga
     6421 ttggcggcgc gtttacggtt cgggacaatc cgtctctacc cgcctgtgaa ccggagcagc
     6481 tgcggaacca aattggcgca tcgaatatcc agggccccgt gctgatgtcg ggcaacaacg
     6541 gccagggccg atgcgggcta ctctgaagat acaccgggta gcgacgctgc tatgagccct
     6601 gcacgtcgac gatgcagaaa tcgaattgtt tggcagccgc attgtcgccg agcacgaaaa
     6661 tgtcgatcgc caacacgggt tgccccgtat atgggatcgc ctcttcgggc gagtaacaga
     6721 acttctggaa ggacgagatg gggatattca ggagaccggc gtttgcggtc gtggcatccg
     6781 ccgcgctgag cgtgtagcag tagccctcgg tttggtcgcc gggctgcagt gagacgcgca
     6841 tgcccgcggt ggccccggcg agctgaagct ttacggacgc tacgctcacc ggcttcgcct
     6901 cgtcatcgcg cgcctgggcg atgctccacc cgatgccagc gcccgagctg tcgtaggccg
     6961 caatgctgcc ggcggcgcag agctgattgc ccacgaagca gctcatcgtt tcggtgggta
     7021 tgggagtgac gaccacgccc gcaccgtacg agtacgtata cgcgtgaccc atccaagtgc
     7081 cgctcacgcc gtaaccattg acgaagggct tactcgcgac cttcagcgcg cccttgtacg
     7141 gcgtgcattc cgcgacggcg ctgcctcccc cagttccacc agctccacca gctccaccag
     7201 ctccaccatt cgtgctcgac gtgcctcctc ggctcgacga tgtaccgccc tggggagccg
     7261 acgccgtacc gcctgagccc gctgacttgc ctcccgtcgc cgcagcgcag ggctcacaca
     7321 tgctgccgcc gcagtcggtg cctgtttcgt cctgatcttg aatcccgtcg taacaaccga
     7381 gatccacgca gcgacggttc aagcaaatct ggccgccgtt gcactgcgcg ttgatccgac
     7441 actcctgcgg gttgcccgct tccccggcag cttgcgtgtc tccggccgcg ccgccgcctc
     7501 cgctcgttcc gccggcggaa tctttgccgc ccgctcctga tgtactgctc gaacccccgg
     7561 agcccttgaa gacgtgatcc gcatccacac tgcagtgggc cagcaggagt actgcactca
     7621 gcaggcatgc ctcgctggcc tgaaaaagaa agtgctggcc gatgcagcgc ttgactgggg
     7681 cagagggggc gtgtcgccag cggggactca tcgcgggagg atgacacacg gcccgcgacc
     7741 ggcgtacaag gtttttggcg tttctggcgc ttggaattgc gtgccaatcc gctcacattg
     7801 cgtaccgtgc ttctcgcttc tgaactgggc tgtcgccggg ttgttttcaa acggctgctg
     7861 ctcgagagct gcttttcgac ggtgttgcgc acgagctgtg ttgccctcca aagaggagat
     7921 ggctttctga gcgcgagccg aacggaatgg ccgctgaacc gaactacgct ctcagtgagc
     7981 ctctgttttc tcgaatcgat cactccattc ggaactctcg aagcgtagga cgatattgcg
     8041 actgacgaac ttgcttgaag tctcgggtga ggttcatggt ccgcctccag aaccgaaaac
     8101 ctcggtgcgg catggtcctc ctcggcccgg atttcacgcc gacggcggac accaaaaatc
     8161 aggctgtgag acccgcagaa tggccgggag cctatgatag tgatggtcaa atggcgacag
     8221 cggcaggaat tcgggattcg cggacagccc tggtgtgggg cccagtgtct agagccttga
     8281 tattggtggg tctactggcg ctccctagct gtcgggagcg gcgctcgatc cagaagtctg
     8341 agcagaagcc tggcccgcgc cctgctgccg cgctcgccgc ctctgcgtcc gttacgaacg
     8401 cagaggctgc tccgcaggga ccgccctcgg caccgaagtt accaagtcag ttggggccta
     8461 ccggggcgag cccaggaccg gcgccgaccg tatctgcaac tgaggtgcgt caagagcgcg
     8521 tcgcggcgtt ggatcggaag tacgccgcgg cgctcggtcg ggcgcggcgg ctccagcaag
     8581 agggaaaggt ggaggaagcg cggaaggcct tcatcaccgc ggcgggagag ggtgaagacg
     8641 tttatcatcg cgcaatcggt gagcttgcgt tcctggaatt caagcacgcg atggggtcgg
     8701 accaggaggt cgaggacacg ctctgggtgg ctgctgccag cgaggatccg gtcgtggccg
     8761 gtcaggcctt cttcaacctc gcagcgctgt atgcacgcca gggcaaagcc gagcctgagc
     8821 gcgcggccct tgctcggtcg ctcgctcgta acccgagcgt tgcggcggag aagaagttgg
     8881 gtcggcgctc tcgttgtgcc gtcgaggtgg gcagcggccc tgatgccgac gcgaaagtcg
     8941 tgaccggctg ggcaggggtt tgccaggcgc tcgatcgctg cgacgcgaag caaccattgg
     9001 atgaagcgca cgcgcgtgcc cgctcgtgcg tcacgacgac agcacgggct ggcgaacccg
     9061 aagaggtgca tggctgcacc gaagagccgc cgtgggactc gacctacaac tactcgctct
     9121 atcggtactg gcacggcatc atccagccgc tgccgagcca ccagtattgg gtgtatcggg
     9181 ccggcgaagg gggttggtcc tcggtttgta gcggcgacca gcggtccacc gcaagtctgg
     9241 tgcagggggt gctttggctc cattacacga gtacttcgtc ccaagtcggg cgtgaccgaa
     9301 tggcgcccca agcaggaggg gtatgctggg acgcacccga ggacaacacg catactttct
     9361 actcgaagac taccggtaag aagctagcga cgatacgagt gctatcccaa gacgaagtcc
     9421 aggtggaggt ctcgccggat ggtcgcagcg tccgcctggg tggagcacac tgttccgggc
     9481 gggtaccgct cgatggttcc aaccggctgc tcgcggatcg gtgaattttg cgagaaggag
     9541 cgcgcgcagg gaggctgagg gcctgcgtgg tgcggagggg gaatgcgtgg tcccagggct
     9601 gcgctagcga gttcacgcga tgggcggcgc gcgcgaaaac aaactggccc caaaaaagcc
     9661 tcggaatggg tgccatgccg taccgttccc gccgatgggc ctagctctgc tgcacctttg
     9721 ggcctgacgg cttatgcttg ccgcgatgca ggatgatacg ttgcggtact gtgtcgcgtg
     9781 tcgcgagacc ttgccggggg aggcgcggtt ctgtccggca tgtggccatg actctacgtt
     9841 acccgagtgc cttccgagcc cagccgttgc acgtcagcag gtggggcgac gcgacgtgga
     9901 tccggagccg gatctgccac ccatgcgtct agcgccggga acgcgccttt cggtttatcg
     9961 catcgagtcg gtgttgggag agggggggat gggtgtggtc tatcgagcct acgacgaagc
    10021 cttgagtcgg ccggtagcaa tcaagtgtct gcatacgaac ttggcggggg acccagagat
    10081 tcgtcggcgc ttcgagcgcg aagcgcggtt gctgcggacg tactcacatc caaacgtggt
    10141 cagtgtgttc gatttcgtcg agtacgagta cctgttggcg atcgtgatgg aactcgtgga
    10201 ggggccgact ctgattcgat atctgcagag ttggcgcggt cggccgccgc tcggagaagt
    10261 tcgtgatcta ttcggcggcg tactggatgc catggaagcg gggcatcaac gcggcatcat
    10321 tcatcgcgac ttgaagccgg aaaatgtgct gctgatgcgc gtcggtgcgg gcttgcgtgc
    10381 caaggtggtc gatttcggaa tcgccaagat tctggagggc acgacctata ccgtgagcgg
    10441 gatgttcttg ggcacg
//
LOCUS       DASTKU010000426         5740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1641977, whole genome shotgun sequence.
ACCESSION   DASTKU010000426 DASTKU010000000
VERSION     DASTKU010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641977"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..463)
                     /locus_tag="VFQ61_14615"
     CDS             complement(29..463)
                     /locus_tag="VFQ61_14615"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9955743.1"
                     /translation="MDLVAHAGVVIRAPRSAVWRALLAPESIPRILPVTEVVEPWRLG
                     RRFVWTFQLAEVEARVEGRVQRVEVEQLLEYDYVDPHSLHVLRKSNVHGVLIELSDHE
                     LGTQVTVTQDANLSAAAHAHAEGGWRLALANLKELVERAPRE"
     gene            1556..3310
                     /locus_tag="VFQ61_14620"
     CDS             1556..3310
                     /locus_tag="VFQ61_14620"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9955744.1"
                     /translation="MPAATMKPERAETHGDPTGMGAREGLTSSRYRVLAELGVGGMAE
                     VYVAVQLGQAGFRRLVVLKRLRQHLVQEPDLLQMFLQEARLAARLSHPNVINTHEVGH
                     DGEGHFMAMDYLPGHTYHALLLRAGRESLDFRVSLKILIEALKGLEYAHELKDFDGTP
                     MGVVHRDVSPGNLLVTYDGQVKVLDFGIAKIVNSSVETQTGVIKGKVSYMSPEQASCT
                     PVGRETDLFAIGVLLWEAAAGRRRWQGLPDIAVLKMLLGREQFSPPCAAQRGLPPEVD
                     TICMRAMAYEPADRYRSAREFRLDLERLAETVGNPVSQEQLGALVAELFADDRRQFQG
                     KIEQQLKRLQKEEDLTVSGLLNPGSGSRSDVFHVGRATLGETTREETQPGPPERSPVR
                     TRRTMAMIAILSAMAAGAATMALSTREPSPQGKLAASEAAPPPSSAVKQTEITLSTSV
                     TPAHAKVYLDGKQLETNPAILKLPRGVATSTVRAEADGFVEKTITFTHEDDRVIQWAL
                     APTETKEEPAARAATSRPQRGLRSGQRAPSADAEPGRAAVAPVVASSAPLPDKSAISP
                     SRKRPKASVSLDTENPWQ"
     gene            3378..4418
                     /locus_tag="VFQ61_14625"
     CDS             3378..4418
                     /locus_tag="VFQ61_14625"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9955745.1"
                     /translation="MRNARLTSLAVVCGLLASFSPALAQTAASAESGKAADGSKPAEV
                     KQSDAGVQQREEAQLRYSRALELFNEGSFEAASLELRRAYELAPSFRILYNIGLVNTE
                     LNDYAAALSAFERYLADGGPNVPPERQAEVRQQIERLSGRVGFLTVTTDPAGADVTLD
                     DLPLAKSPIATPIRVNAGRRRISVAMTGRLPQTRVIEVAGGDRVNLTFELNEPQSSAP
                     PSRPLLTDLQPPSSSPAAPSAPPPRAVPWAMWGVTGALAVGAGTLGIIALRERSDQKS
                     LQEKFGVTESELDHADTRVRRFALLTDVLMGATVIAGGVSLYLTLRTPNSGEGQVALA
                     VRPNGVDARLAF"
     gene            4507..>5740
                     /locus_tag="VFQ61_14630"
     CDS             4507..>5740
                     /locus_tag="VFQ61_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955746.1"
                     /translation="MNRRFTPKTVALASLFTLSGCSLLLKNDGKQCSTNADCSALGFQ
                     GSVCDIPAGVCKKTDTEGSGGQRSSGGANSSGTNSTAGTTQMVEGGAGGGRSATEGGA
                     GAGESGAGGDAGAAGAGGQSTIPPECGDQCSVPVGIRVNQTELLTYAGSSTAGVLHSD
                     VCPLDQVAIGVRSYIGTRVNSTFLQRMQVLCGTIVLSNARTGALTVKAGAATPVRGEG
                     FPDDTRVDRVCPANQVVVGHAAYYNSGGAVESLQLRCAPLAVEGAPGSYTVVTGTAQD
                     LEWAGPTTAANKTKAVDCAAGAIARGLQVRAGTVVNGLAAICGVPSYTYPVGANCAGA
                     SDCDSLNCSDNRCAKSSCEPSSDCSCSAFESRWYSFCKTPSGFYAALEVCRTSEGMLA
                     QVQDTFENGWLRLTANELK"
BASE COUNT         1066 a         1771 c         1831 g         1072 t
ORIGIN      
        1 ttcgggatcg ttcgagatct cccgagtttc attcacgagg agcgcgttcg acgagctcct
       61 tcaggttggc gagcgcgaga cgccagccgc cctcggcgtg cgcgtgggcc gccgcggaga
      121 ggtttgcgtc ctgcgtgacc gtcacttgcg tgccgagctc gtggtctgaa agctcgatga
      181 gcacgccgtg cacgttgctc tttcggagca cgtggagcga atgcggatca acgtagtcgt
      241 actcgagtag ctgctcgacc tccacgcgtt gcacacgccc ttccacccgc gcttcgacct
      301 ccgcgagctg gaacgtccac acgaaccgcc gtcccagccg ccaaggctcc acgacctccg
      361 tgactggcag aatgcgtggg atactctccg gtgcgagcaa ggcgcgccat accgcgctac
      421 ggggggcgcg gatgactact ccggcgtgcg cgacgagatc cacacctgcg ttttgtaggc
      481 agtctccgta agcgcaagct tcttgtgcga gctcagctgc gaagacgcga gccgtgcgtt
      541 ggcgaacgcg ctgcgcgtgg cttccccacg ccgccgggcg cggtgagacg ttgtctcgct
      601 cgcaaagcga atcgcggtgc tgcggaccgc gtgaaaatca gtgactggta ctcgagttcg
      661 cgaactaggg gtcaaaattg accccattgg ggactccgtc cgaccgacct cgttcgtccg
      721 tcctcgctag ttggctacat ggttaactcc gccgaggtca ccggtggtac tgacctggcg
      781 acgtcctcgg ctgtccgttc tcaaactact ccgtgcgacc agcatcgagc ggcgtcactc
      841 ttggtacgcg ggtggcgcct cggttccgca ccggtgaacg ctgacgtggt ttccagcagt
      901 tttctcagtc gttgggagcc ggccgagcaa cttactcact cctgccagta gcaaacgctg
      961 gtggttcgcg tcgcgatagc ccgctgacca aggggtttgc acacttgcga tgactcggtc
     1021 tcgtttgttg cctccgcggt gctcgaggag gcggttgccc ggtcttcgac cgcctcgtct
     1081 aaccatcgaa tgcggcatcc ctcaatcagt tcagtattgc ggggcgcact gcgggccccg
     1141 cacgatggcg accgcttttc aacttcgttt tggcgtgaag ctctgcgggc cgagcgtcgc
     1201 cagtcgcgtc gcgttagatg cgccatgatc gagccctgac ccgatggaaa caactctaat
     1261 ttgtgagccg aagtgcagct ctcacgccgt caccccccgc tgcttgccct ctgtcggcgg
     1321 gaggggctga acgacgcctg ctcgagccgc accgctccgg ctcggcaagt taccagccta
     1381 tgtataagcg catggttcgt cggccgcgtg tcttgccatt ctggacgtga cgtgcgctaa
     1441 tatggagttg ttttcaggcc tttcaccgcc aacttcggcc tcgtttgttc agcgggtaag
     1501 gtcctattcc tccagaccgt ttggatggtt cagggaccaa ctagggaaat ctggaatgcc
     1561 agcagccaca atgaaacccg aacgcgcgga gacccacgga gacccaactg gaatgggtgc
     1621 tcgtgaaggg ctcacttcga gccggtaccg cgtactcgct gagctcgggg tagggggaat
     1681 ggcggaggtg tacgtcgccg tccagctcgg ccaggccggg tttcgtcgtt tggtggttct
     1741 caaacggcta cgccagcacc tcgtgcaaga acccgatctc ctgcagatgt tcctccaaga
     1801 ggccaggctt gcagcgcgcc tcagccatcc gaacgtaatc aatacgcacg aggtaggaca
     1861 cgatggtgaa ggccatttca tggccatgga ctacctgccc ggccatacct accatgccct
     1921 actactgcgt gccgggcgtg agtccttgga cttccgcgtt tcgctgaaga ttttgatcga
     1981 agcgctcaaa ggactcgagt atgcgcatga gttgaaggac ttcgacggaa cgccgatggg
     2041 cgtagtgcac cgggacgttt cccctggcaa cctgctcgta acttacgacg ggcaggtcaa
     2101 ggtactcgac tttggtatcg cgaagatcgt gaattcgagc gtcgagacac agaccggggt
     2161 catcaaaggc aaggtctcgt acatgtctcc ggagcaggca agctgcacgc ccgtggggcg
     2221 cgagacggat ctattcgcga tcggcgttct tttgtgggag gccgcagcgg gtcggcggcg
     2281 ctggcaaggg ctcccagata tcgccgtgct gaagatgttg ttggggcggg aacaattcag
     2341 ccctccttgc gcggcccaac gtggtttacc tcctgaagta gacacgatct gcatgcgggc
     2401 tatggcgtac gagcctgccg atcgctaccg aagtgcccga gagttccgct tggatttgga
     2461 gcgcctcgcg gaaaccgtgg gcaacccagt cagccaagag caactcggcg ccttggtcgc
     2521 ggaactcttc gccgacgatc gccggcagtt ccaagggaag atcgagcagc agctcaagcg
     2581 ccttcagaaa gaagaggacc tcaccgtctc ggggctactc aacccgggat cgggctcacg
     2641 cagtgacgtg ttccacgtcg gtcgagcaac gctgggagag actacgcggg aggagacgca
     2701 gcccgggccg ccagagcgtt cccccgttcg aacgcgtagg accatggcca tgatcgcgat
     2761 tttgagtgcg atggcggcgg gcgcagcgac catggctttg agcacgcgcg agcccagtcc
     2821 gcagggcaaa ttagcggcga gcgaggctgc tccacccccc tccagcgccg taaaacagac
     2881 agaaatcacg ctcagcactt cagtaacccc ggcccacgcg aaggtgtact tggatggcaa
     2941 gcagctcgag accaaccctg cgatcctgaa gttgcctcgg ggcgtggcga cgagtaccgt
     3001 gcgggccgaa gcggatggat tcgtggagaa gaccatcacc ttcacgcacg aggacgaccg
     3061 ggtcattcaa tgggctttgg cacctacaga aaccaaagag gagcctgcag cgcgcgccgc
     3121 cacctccagg ccgcagcgag gtttgcgtag tgggcaacga gcgcccagcg ccgatgcaga
     3181 gcccggccgt gccgcggttg ctcccgtggt cgcttcatcc gctccacttc cggataagtc
     3241 ggcgatctcg ccctcgcgca agcgacccaa agcctcagtg tctttggata ccgagaatcc
     3301 ttggcaatag cgtgcctctc cccggggcgt cagaacgtgg gaagtcaatg agaacgatga
     3361 aacacggtca gctgaggatg agaaatgctc gcctgacttc gttagcggtc gtgtgcggcc
     3421 tacttgccag cttttcgcca gcgctcgctc aaaccgcggc ttcggcggag agcggtaaag
     3481 cggcggacgg tagtaagccg gcggaggtaa aacagagcga tgcgggggtc cagcagcgcg
     3541 aagaggcaca gcttcgctat tcgcgggcgc tcgagctctt caatgaaggt agcttcgaag
     3601 cggcctcgtt ggagctgcgt cgcgcgtacg agctcgcccc gagctttcgt attctctaca
     3661 acattggcct ggtcaacaca gagctcaacg actacgcggc cgccctcagc gctttcgagc
     3721 gctacctggc ggacgggggc ccgaatgttc cgccagagcg ccaggccgag gttcgtcagc
     3781 agatcgagcg cctctcgggg cgcgtggggt tcctgacggt tactaccgat cccgcgggcg
     3841 ccgacgttac tttggatgat ctaccacttg cgaagagccc gatcgcgacg ccgatccgag
     3901 tcaatgccgg acgccgacgg atctcggtgg cgatgacggg tcgacttcca caaacccgcg
     3961 tcatcgaggt cgctggtggg gaccgggtca acttgacgtt cgagttgaac gaaccgcaat
     4021 cctcagcgcc gccctcacgg ccgctgctca ccgatctgca gccgccgtcc tcgagccccg
     4081 ccgcgcctag cgctccgcca ccacgcgcgg tgccctgggc gatgtgggga gttaccggcg
     4141 cgttggcggt cggagcggga accctgggga tcatcgctct gcgtgaacgc tcggatcaaa
     4201 agtccctgca ggagaagttc ggggttaccg agtccgagct cgaccatgcc gacaccagag
     4261 ttcgtcgctt cgctctgctc acggacgtgt tgatgggagc aaccgtgatc gccggtggcg
     4321 tctcacttta tctaacgctc aggactccca actcgggaga aggtcaggtc gcgcttgcgg
     4381 tccgacctaa cggcgtcgac gcgcgcctgg cgttctgatg agcgtttttt cccgtgcctc
     4441 tcaatcggcg cacaactcag aatgcttttc cggccatccg catatccccc tttggtgagg
     4501 cttctcttga atcgccgctt tactcccaaa acggtagccc tggcatcgct cttcacgctc
     4561 agcggctgct cgctgctgct gaagaacgac ggcaagcaat gctcgacgaa tgcggattgc
     4621 tccgctctgg gtttccaggg cagcgtctgc gacatccccg cgggcgtgtg taagaaaaca
     4681 gataccgagg gctccggcgg gcaacgaagc tccggtggag caaactccag cgggacgaac
     4741 tctaccgcag gcacgacgca gatggtcgaa ggcggagcag gcggcgggcg atccgcaacc
     4801 gagggcggag ctggagcggg agagtccggc gctggcggcg acgcaggagc ggccggagct
     4861 ggtggacaga gcacgatccc gccagagtgc ggagaccagt gctcggtgcc ggtcggcata
     4921 cgggtcaatc aaacggagct cctgacctac gcggggtcga gtacggccgg tgtcctgcac
     4981 tcggatgtgt gcccgctcga tcaggtcgca atcggggtac gctcgtacat cggtacgcgg
     5041 gtgaactcca cgttcctgca acgcatgcag gtactctgcg gcacgattgt gctgagtaac
     5101 gctcgaaccg gcgcgctcac agtcaaagcc ggagcggcca cgcccgtgcg cggcgagggg
     5161 tttcccgacg atactcgggt agaccgtgtt tgcccggcga accaagtagt ggtcggacac
     5221 gcggcttact acaactccgg cggtgcggtg gagagcttgc agttacgctg cgcaccgctc
     5281 gccgtagagg gggcgccggg ctcgtacaca gtcgtcacag gtacagccca agatctagag
     5341 tgggcagggc ctacgaccgc cgcaaacaag accaaggccg tggactgcgc ggccggcgca
     5401 atcgcgcgcg ggctccaggt tcgcgccgga accgtggtga acggcttggc cgcgatctgc
     5461 ggagtaccaa gctacaccta tccggtaggg gccaactgcg cgggagccag cgactgcgat
     5521 agcttgaatt gcagcgacaa ccgttgcgca aagtcctcgt gcgagccctc ctcggattgc
     5581 agttgcagcg cgttcgagtc caggtggtat tcgttctgta agacgccgag tggcttttac
     5641 gctgcgctgg aggtgtgtcg aacctccgaa ggcatgctgg cccaggtgca ggataccttc
     5701 gaaaatggct ggctgcgttt gaccgccaac gaattgaagc
//
LOCUS       DASTKU010000427         9530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1420207, whole genome shotgun sequence.
ACCESSION   DASTKU010000427 DASTKU010000000
VERSION     DASTKU010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9530
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420207"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VFQ61_14635"
     CDS             <1..1248
                     /locus_tag="VFQ61_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955747.1"
                     /translation="PRVARLEQLDRYGEGTLLFRPGLPAEFARKVVAEPESSSATLEL
                     SLAFGLDSPLPQATWTVASRRAALSSLASLPGLSPFLPPARRERLQSMFDALSCEPRL
                     RGESGLATRIGLSRDSSGFELLAEIAAEPLASAESSLAAASTWIAEQSRATASRPLQS
                     AVCDAESGKFSLRTEAQPRPDQDSRSRAPTLDQVERDFEAQPLFARLGSGGTFSQLQR
                     VAPQLAFFVLSLPDLLNQGRPADLHSSVDPMLGSELSAFLDGLERELRQLSRAFLRDL
                     ERLSVPCELGFVYSEEHEPVRRLVYELSAELRGASAHARAGMLAALGLASSLFQRGIA
                     RLVQRLGLSDELEYFDPTRAYGSDLCRRFLTRAPASERMQSCLRIFRELPRLCHHLEG
                     RLCASEFQALGSLKRASLRIREL"
     gene            complement(1289..2242)
                     /locus_tag="VFQ61_14640"
     CDS             complement(1289..2242)
                     /locus_tag="VFQ61_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955748.1"
                     /translation="MFAESLVGFSPRPAFQVLRREPHALRVRVSRAALEPLCEGHFPG
                     DPIVPGAHLFALASDAGALLSSRRSDSLCTKRIGEVTQNEPVNAQFRQIVRPDHDIEI
                     ELCSYPIGALEHGEIRSSIYSVSESDCCARFRCLTRAGSLQRFSCPEPVSERRLNEVS
                     TQHSEGTEQIARLKHRGRALLLERELLVQDEQARLASEGPKQRSFGSRSLDQWHWPLL
                     LDGAAQAAGLIAKDAAGSGTTGLTTSTPSNASESNPTLVVVAYELKHLVRAQYAGRVE
                     FSASFRRRVLAMLELSVVARAPSSGRVLLETNIVLAPKPAR"
     gene            complement(2548..2739)
                     /locus_tag="VFQ61_14645"
     CDS             complement(2548..2739)
                     /locus_tag="VFQ61_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955749.1"
                     /translation="MIRQKVIRDRARLSPTKLLNPNLHAYVAREPGSCREHLAMETSR
                     LRTQAGWSRAPLVLGSKNA"
     gene            2926..4071
                     /locus_tag="VFQ61_14650"
     CDS             2926..4071
                     /locus_tag="VFQ61_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET9955750.1"
                     /translation="MTGRGAVTAYGVGFSEFVAGVETRRGALTEFGARANTRVSEPTR
                     PNWKAGLVTDLSAFRRTFPEIKPPLPASVTQMVMLAAHEALASAGIDTSEAPKPIGMF
                     FNRNRGPAAVVAKAMLPVLQGGAKKMSPLWFSQAVANAPIGALSVAMGLRGPHVLSMG
                     GGALQMAFDELSSERSSVVLCGALEEVEPYSFRAALENGFIGQAEADSSEAVASFGEG
                     AVCFVLESLASARARGAQPLAELVAVEQGLDEEVREDCQLRGFGQVSAAGLERLCRAA
                     MQAAQVRAKDLTFFVGGQNGQPALREAQRGALHALGRPDLAERDPSLSFLVGDGYGMA
                     ALLSVAWALHELNGATKLPPPAPAYALLTHTEFHGQHSAVVMRGVSL"
     gene            4068..4862
                     /locus_tag="VFQ61_14655"
     CDS             4068..4862
                     /locus_tag="VFQ61_14655"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET9955751.1"
                     /translation="MTATLGSLNRELDHTPSSPRKVALVTGASRGIGRAIARGLAQRG
                     CHVVINYAQNQAAAQAVHEELNRAGYACSIARSDVSNWDAVQDLMRSIRGELGRLDIL
                     VNNAGVLEEKLFFFQSLERFWEVMRINLGGTANLCKAATPLLGARKAGRIVNLSSIAA
                     SHGTTGLSAYACSKAGIEALTKVLARELASSGIRVNAVAPGLIQTEMTESLRSASTKP
                     EALRRQPVERLGRPEEVAELVSFLALDAPDYFTGEVLRLDGGAAIA"
     gene            4855..6120
                     /locus_tag="VFQ61_14660"
     CDS             4855..6120
                     /locus_tag="VFQ61_14660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET9955752.1"
                     /translation="MPERIDARRSREPIAITGMAAWTSYGRGLDALLHALATGRVTQH
                     APVGLDVDLDDPFLYARVARQIALPSELKTLWDRSPRPVTPEQWPTEVAVTCAGDALL
                     DAGRPEHHYDRARIAICNGTSHGSNHGLISYLRQSLSREPDPNSIADASSIVARRIAL
                     RSGALGPNYTLNTACSSGINAIGQGMNLLRSGRADCVIAGAHDTFSELSFVGFTSLKA
                     LDPNGCRPFDVQREGLTLADGAAYLVLERIGSARARGRKPLALITGYGCVSEAYHATA
                     PAPDGDGVLRGMLAALSEDGRAEELDLVFAHGTGTPANDAGELKAIQRLTAELDCGNP
                     LDVVSLKSQLGHALGAAGSLQIVAAAACLRAELTPGNIGLQSPISHSSKLRLRSTPAR
                     AVNRLVICNALGFGGSVATACLRLESEAV"
     gene            6149..6466
                     /locus_tag="VFQ61_14665"
     CDS             6149..6466
                     /locus_tag="VFQ61_14665"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET9955753.1"
                     /translation="MTTENISQLAERVAQVIIDRLELDEFNAQTFPKDAILFAKANQG
                     GLGFDSIGSLEIAAGLSDAFNLDLDDASESDFTSVNTLARYIARNLAGAGETTDGSFE
                     AAQ"
     gene            6463..7992
                     /locus_tag="VFQ61_14670"
     CDS             6463..7992
                     /locus_tag="VFQ61_14670"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9955754.1"
                     /translation="MRVEALGHASWLVRGGIASVLFDPLLFGEHQDGLYDVYPPRALD
                     LGSLPEVSALIVSHLHADHFDPDSLAFFPKDMPVIAADDPLLVGCLEGLGFEHILRAQ
                     NHVPIQVGDLSVTPTPASPGAPEHGFLVRHEDVTLWNMVDTFPEPDDIATVLEGLAKV
                     DLLIAPWQPLRDSALSAGEPIQFPYEMYTQILQQIAQIKPEMLALGAAGFRARGAAAF
                     TNHLMFPVTRERFIRDISQMVPALTTACLTLDPGDALTLGPQLVSVEKGLLPYCRTLE
                     PYDDRELAFRPFELGFEVKETRGSGFKRAECFEAVDAFFEQSLLDCVRDRARKFDLHH
                     RFQVIREYEVVFHGGDRAHWVLDFSGPRCELRRGGDPLATAHTVLPASLLVGLIAGTI
                     TWDYAVLSAELRRYDQTYLSNPGGVSRPRPGALFDPLVLVFGTQRAKEYALALALERV
                     QKDWQEASAAAATLPPPPVPAETDAVKVLDPSVIAMDVFQKLGRPMPAERLDPRSESN
                     P"
     gene            7989..8456
                     /locus_tag="VFQ61_14675"
     CDS             7989..8456
                     /locus_tag="VFQ61_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955755.1"
                     /translation="MKNTTRREILGKLGASAAALTTLSVAGVAAAQAQVAKVAPTKAI
                     LATEARTRVKLAPGDATVQINVGIDRKGFILQTQRLTGAEPKVGLTAEGKVLQLGLKI
                     KDGVVDVIGLDSVLNRPGNVAAEGRCTMRNLSQDAVVLPGVDLLKNTKALAKF"
     gene            8471..>9530
                     /locus_tag="VFQ61_14680"
     CDS             8471..>9530
                     /locus_tag="VFQ61_14680"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HET9955756.1"
                     /translation="MWTRSPNPATCWLVNPHLVRVLGSSTEVYLSTQPERTFELTRDT
                     FEFLVAFASARPLHEVLSSPTPDQLQTVAGLVENGLLVPAEAKLAQPTPPRARPVSLR
                     FCGAAPLISRPASPENEASLGDAVVVLGAKYDAGTLDRYHKGASRGPDAIRQESSQYP
                     LFPDLRTGRVLGFPDVEARRYLGAGIRLLDAGDVQAGGGTPNTTWHQGLREELRGLRK
                     RQAKVLLLGGDHSVSLASIQALQGSPLGVLQFDAHTDFGLVEHEAHLTHANVMRHVHR
                     EAHVAHLVCCGLRGFQQLPELEGVERYRSCSVGALRRMTSSELDEVLRPDLPYYVTVD
                     IDVLDAPLVPGTAVPEPDG"
BASE COUNT         1703 a         3073 c         3051 g         1703 t
ORIGIN      
        1 ccgcgggtcg cccggctcga acaactcgat cgctatggcg aagggacgtt gttattccga
       61 ccgggcttgc ccgccgagtt cgcacgcaag gtcgtggccg agccggagtc gagtagcgcg
      121 accctggagc tatctctggc cttcggactc gattcgcccc tgccccaagc cacttggacg
      181 gtggcgagcc gtcgcgccgc gctgagctcg ctcgcaagtc tgccggggtt gtctccgttt
      241 ttgccgccag cccggcgcga gcggttacaa agcatgttcg atgcgctgtc ctgcgagccg
      301 cgcttgcgcg gcgagagcgg gctggcgacg cgcattggtt tgagccgaga cagctcgggc
      361 ttcgagttgt tggcggagat cgcggcggaa cccctggcaa gcgcagagag ctccctagct
      421 gcggccagca cctggatcgc ggagcagtca cgcgcgaccg cgagccgacc actccaatcc
      481 gccgtgtgcg acgcagagtc cggcaagttc tcgttgcgga ccgaagctca gcctcgaccg
      541 gaccaagatt cgaggagtcg cgcgccgacc ctggaccagg tggagcgtga cttcgaagct
      601 caaccgttgt tcgcccggtt gggcagcggc ggcacttttt ctcagctcca gcgcgtggcg
      661 ccgcagctcg cgttcttcgt gctctcgttg ccggatctgc tcaatcaggg ccgccccgcg
      721 gacctgcatt cgagcgtgga tccgatgctc ggctcggagc tgagcgcgtt tttggatggg
      781 ctcgagcgcg agctgcgcca actcagccgg gcgttcttgc gcgacctgga acgcttgagc
      841 gtgccctgtg agctaggttt cgtgtactcc gaggagcacg aaccggtgcg tcgtctagtc
      901 tacgaactgt ccgcggagct gcggggcgca agcgcgcacg cgcgcgcggg catgctggcc
      961 gcgctcgggc tcgcgagttc gctgttccag cgtggaattg cgcggctcgt gcagcggctc
     1021 ggactctcgg atgagctgga gtacttcgat ccgacgcgcg cgtacggctc cgacttgtgc
     1081 cgcaggttct tgacgcgcgc ccccgcgagc gagcggatgc agtcatgtct gcgcattttt
     1141 cgagaactgc cgaggctctg tcatcacctc gagggccggc tctgcgcctc cgagtttcag
     1201 gccctcggca gcttgaagcg agcatcgctg cgcattcgcg agctctgatc gagccccact
     1261 ctgaccgcgg cgagccgcca tcgcaaggct atcgagcagg cttgggcgcc aacacgatgt
     1321 tcgtctcgag caacacgcga cccgagctcg gagcgcgcgc gacgaccgag agctccagca
     1381 tggcgagcac acgacgccgg aaagatgcgc tgaattccac gcgccccgca tattgcgccc
     1441 gcaccaagtg cttgagttcg taagcaacca ccaccagggt ggggttcgac tcgctcgcgt
     1501 tcgacggcgt cgaggtcgtc agtcccgtgg ttcctgaacc ggccgcatcc ttcgcgatga
     1561 gtcccgccgc ctgcgcggca ccatcgagca gcaacggcca atgccattgg tcgagcgagc
     1621 ggctaccaaa cgaccgttgc ttcggtccct cgctcgccaa gcgcgcttgc tcgtcctgga
     1681 cgagcaactc gcgctccagc aacagcgcgc gcccgcgatg cttcagacgt gcaatttgct
     1741 ccgtgccctc cgagtgttgc gtcgagactt cgttcaatct gcgctccgaa acaggttcgg
     1801 ggcaggagaa ccgctggaga gagcctgccc gcgttagaca acgaaatcga gcacaacagt
     1861 cggattccga cacggaatag atcgaactgc ggatctcgcc atgctccaag gcgccgattg
     1921 ggtaactgca caactcgatt tcgatatcgt gatcaggcct cacgatctgt cgaaactgcg
     1981 cgttcactgg ctcattctga gtcacctcgc cgatacgctt ggtgcagagc gagtccgacc
     2041 ggcggctcga aagcagcgcg ccggcgtccg aggccaacgc gaacaagtgt gcgcccggca
     2101 cgattggatc gcctggaaaa tgcccttcgc acaagggctc gagagcagcg cgcgaaacgc
     2161 gcacgcgtaa ggcatgcggc tcgcgccgaa gcacttgaaa tgccggacgc ggtgagaatc
     2221 caaccagaga ctctgcgaac atcgccagca cgatagtgca ttggcgtgaa gcgcgggcac
     2281 cacttgtgtt ctcacacctc gacttaggag gtgggatctt tcgaaaacct cgctgcagaa
     2341 gattgccact ctcggcttcg aatcgtttat gatgcgcggt ctagagggtc accactgtag
     2401 gcaaacgcac gcgcgaaggt acaaggcaca gcgcgaaggc acattgggca aatgtcacat
     2461 tgcgcaagtc caggcaggcg gagctgtgcc atggagtatc gaatacaaga caaatggtta
     2521 ctgaggattc gaccactcac gatccaacta cgcattcttc gagcccaaca cgagcggtgc
     2581 gcgcgaccac cccgcctgtg tacgcaggcg gctcgtctcc atggccaggt gttcacggca
     2641 cgagcctggc tcgcgcgcga cgtaggcatg cagatttgga ttcaatagct tcgtagggga
     2701 gagccgagct cggtctcgaa tcaccttctg tcggatcaat cattttccta ggccgatctc
     2761 gcattggcct tgtcaacttc gcgcgtcgtc ggcgcgaatt ccaggtcgaa atgatttgga
     2821 atcgacccgg cgttcctcaa tcacttggag cttcgacaaa ccgtggagcc tgcgattcaa
     2881 tcgacagaac ccgtggttcg ggcaacagag cggcaacgca tcgttgtgac cgggcgcggc
     2941 gcagtcacag cctatggcgt gggatttagc gaattcgtgg ccggagtcga aactcggcgt
     3001 ggcgcgctca cggagttcgg tgcgcgcgcc aacacgcgag tgtccgagcc cacgcgcccc
     3061 aactggaaag caggcttggt tacggatctg tcggcgtttc ggagaacgtt ccccgaaatc
     3121 aagccaccgc tacccgcgag cgtcacgcag atggtgatgc tcgccgccca tgaggcactg
     3181 gccagcgccg gaatcgatac gagcgaagcc cccaaaccga ttgggatgtt tttcaatcgg
     3241 aaccgcggcc ccgccgccgt ggtggccaag gcgatgctgc ccgtactcca aggcggcgcc
     3301 aagaaaatga gcccactctg gttttcacag gcggtagcga acgcgccgat cggagcccta
     3361 tcggtggcca tgggattgcg cgggccgcac gtattgagca tgggtggcgg cgcgctgcag
     3421 atggcgttcg acgagctaag cagcgagcgc tcttcggtgg tgctgtgcgg ggcgctggaa
     3481 gaagtagagc cctacagctt ccgcgccgcg ctggagaacg gctttatcgg ccaagccgaa
     3541 gcggacagct ctgaagccgt ggcgtccttc ggggaaggag ccgtctgctt cgttctagag
     3601 agcttggcca gcgcgcgcgc acgcggggct caacccttgg cggagctcgt tgccgtagag
     3661 cagggcttgg acgaagaagt gcgggaggac tgccaactac gtggcttcgg ccaagtgagt
     3721 gcggcggggc tcgagagact ctgccgggcc gccatgcaag cggctcaggt gcgcgcgaaa
     3781 gatttgacct tcttcgtcgg cgggcagaat gggcaaccgg cgctgcgcga ggctcagcgc
     3841 ggagcgttgc acgcgctggg ccgtcccgac ttggccgagc gcgatccgag cctctcgttc
     3901 ctggtcggcg atggctatgg catggcggcg ctactgagcg tggcgtgggc cctccacgag
     3961 ctcaacggcg cgaccaagct accgccgcct gcgccagcgt acgccttgct cacccacact
     4021 gagtttcatg gccagcacag cgcggtcgtg atgcgtggag tttccctatg accgcaacgc
     4081 taggatcgct gaatcgagag ctcgaccaca ccccgtcctc ccctcgtaag gtggccctgg
     4141 tcaccggagc ttcacgcggg atcgggcgcg cgatcgcgcg ggggcttgct caacgcggct
     4201 gtcacgtcgt gatcaactac gcccagaacc aggccgcggc gcaggccgtg cacgaggagc
     4261 tcaatcgagc ggggtacgcc tgctcgatcg ctcgctccga cgtgtccaac tgggatgccg
     4321 ttcaagacct aatgcgcagt atccgcggcg agctcggccg cctggatatc ctggtcaaca
     4381 acgcgggagt cctggaggag aagctgttct tcttccagtc tctcgagcgc ttctgggagg
     4441 tgatgcggat caacctgggc ggcacggcga atctttgtaa agccgcgact cccctgctcg
     4501 gtgctcgcaa ggccggacgc atcgtgaatc tgtcctcgat tgccgcgagc cacgggacga
     4561 cgggcctttc ggcatacgcc tgctccaagg cgggaatcga ggccctcacg aaagtgctcg
     4621 cgcgcgaact cgcgagctct gggattcgcg tcaacgccgt agctccgggc ctgattcaga
     4681 ccgaaatgac cgagtctcta cgcagcgctt cgacgaaacc cgaggccctg cgtcggcaac
     4741 cagtcgagcg acttgggcgc cccgaagagg tggctgagct cgtcagcttc ctggcgctcg
     4801 atgcacccga ctacttcacg ggcgaggtct tgcgactcga cggaggcgcg gccattgcct
     4861 gaacgaatcg acgcgcgccg atcgcgggag cccatcgcca tcacgggcat ggccgcatgg
     4921 acctcttacg ggcgcggtct agatgcattg ctccacgcgt tggcgacggg ccgcgtcacg
     4981 cagcacgccc ccgtcgggct ggacgtcgac ttggacgacc cgtttctata cgcgcgcgtt
     5041 gctcgccaga tcgcgctacc gtcggagctg aagacgctgt gggatcgcag ccctcgtccc
     5101 gttactcccg agcagtggcc cacggaagtg gcggtcactt gcgcgggtga cgcgctgctc
     5161 gacgcgggcc ggcccgaaca ccactacgac cgggcgcgga tcgcgatctg taacggcacg
     5221 tcacacggtt cgaatcacgg cttgatttcc tatcttcgac agagcctgag ccgtgaaccc
     5281 gaccccaact cgatcgccga cgcctcttcg atcgtcgcgc gtcgaatcgc gttgcgcagc
     5341 ggcgccctgg gcccgaacta cactctgaac accgcctgct catccggcat caacgcgatc
     5401 gggcaaggca tgaacctgct acgcagcggc cgggcagact gcgtgatcgc aggagcacac
     5461 gatacattct cggagctcag cttcgtcggc ttcacgtcgt tgaaggcgct ggatccgaac
     5521 gggtgccggc ccttcgacgt acaacgcgag gggctgacgc tggccgacgg cgccgcctac
     5581 ctggttctcg agcggatcgg ctcggcccga gcacgcggcc gcaaaccgct cgcactcatc
     5641 acgggctacg gctgcgtgag cgaggcctat cacgccaccg cgcccgcgcc ggacggcgac
     5701 ggcgtgttgc gcggcatgct cgcggcattg agcgaagacg ggagagccga ggaactggac
     5761 ctggtattcg cgcatggcac gggaactccc gccaacgacg cgggcgagct gaaagcgatc
     5821 cagcggctca cggctgagct cgactgcggc aacccgctcg acgtggtctc gttgaagagc
     5881 cagctcgggc acgcgctcgg tgcggcgggc tcgctgcaga ttgtggcggc ggcggcctgc
     5941 ttgcgcgcgg agctgacgcc aggaaacatc ggcttacaga gccccatctc gcattcgagc
     6001 aagctgcgct tgcggtcgac tcccgcgcgc gcggtgaacc ggttggtgat ttgcaacgcg
     6061 ctcggcttcg gcggcagcgt cgccaccgcc tgtctgagat tggaatcaga agcagtgtga
     6121 atcggaagca gcgtgaatcg gaggcagcgt gacaactgaa aacatttccc aactcgcgga
     6181 acgtgtggct caagtcatca tcgaccggct ggagctggat gaattcaacg cgcagacatt
     6241 cccgaaggat gccatcttgt tcgccaaggc gaaccagggc gggctcgggt tcgactcgat
     6301 aggtagcctc gaaatcgcgg ctggtttatc cgacgctttc aacctggact tggacgacgc
     6361 gagcgagagc gacttcacct cggtcaacac gttggcccga tatatcgccc gaaacttggc
     6421 cggcgcgggc gagaccaccg acggaagctt cgaggctgcc caatgagagt cgaagccttg
     6481 gggcatgctt cttggctcgt ccgcggggga atcgcgagtg tactcttcga tccgctgttg
     6541 ttcggcgagc accaggatgg tttgtacgat gtatacccgc cgcgcgcgct cgatttgggg
     6601 agcctgcccg aggtctcggc gctgatcgtg agtcacctcc acgcggatca cttcgaccca
     6661 gactcgctcg ccttctttcc caaagacatg ccggtgatcg ccgccgatga tccgctgctc
     6721 gtggggtgtt tggaggggct cgggttcgag cacatcctgc gcgcacagaa tcacgtgccg
     6781 atccaagtcg gcgatttgag cgtcacaccc acgccggcgt cgccgggcgc acccgagcat
     6841 ggcttcctgg ttcgccacga ggacgtgacg ctgtggaaca tggtggacac ctttcccgag
     6901 cccgatgaca tcgcgaccgt gctcgagggt ctggcgaagg tcgatctgct gatcgccccc
     6961 tggcagccac tacgagacag cgcgctctcg gcgggagaac ccatccaatt cccttacgag
     7021 atgtacacgc agattctgca acaaatcgcg caaatcaagc ccgaaatgct cgcgctcggg
     7081 gcggccggtt ttcgtgcacg cggcgccgcc gcattcacga atcatttgat gttccccgtg
     7141 acgcgtgagc ggttcatccg cgacatctct caaatggtgc ccgcgctcac caccgcctgc
     7201 ctgacgctgg atccggggga cgccctgacc ttgggcccgc agctcgtttc ggtggagaaa
     7261 ggcttgctgc cctactgtcg aaccctcgag ccgtacgacg accgtgaact cgcattccgc
     7321 cccttcgagc tcggcttcga agtcaaggag acccgcggca gcgggttcaa gcgtgccgaa
     7381 tgcttcgagg ccgtcgacgc attcttcgag caatccctgc tcgactgcgt gcgcgatcga
     7441 gcgcgcaagt tcgatttgca tcatcgattc caggtgattc gcgagtacga ggtcgttttc
     7501 cacggcggag atcgggccca ctgggtcctc gatttttccg gtccgcgctg tgaactcagg
     7561 cgtggcggag acccgctcgc gacggcccac accgtgttgc cagcgagcct gctcgtaggc
     7621 ttgattgcgg gaacgatcac gtgggactac gcggtcctga gcgccgagct gcgtcgctac
     7681 gaccagactt atctttccaa tcccgggggc gtgtctcgcc cgcggccggg cgcgctcttc
     7741 gatccgctgg tgttggtctt cggtacgcaa cgagcgaagg aatatgcgct cgctttggcg
     7801 ctcgaacggg tgcagaagga ttggcaggaa gccagcgccg cggctgcgac tttgccaccc
     7861 ccgccggtcc ccgccgagac tgacgccgtc aaggttttgg acccatccgt gatcgccatg
     7921 gatgtgttcc agaaactcgg caggcccatg cccgccgagc gcttagaccc tcgatcggag
     7981 agcaatccat gaagaacacc acacgcagag agattctcgg caagctcggc gcttccgcgg
     8041 ccgcgctcac tacgttgtcc gtggcgggcg tggccgcggc ccaagctcaa gtcgccaaag
     8101 tcgcgccgac caaagccatt ctcgcgaccg aggcccgcac gcgagtgaag ctcgcccccg
     8161 gagacgcgac cgtgcagatc aacgtgggca tcgatcgcaa aggcttcatc ctccaaactc
     8221 aacgtctgac cggcgccgag cccaaggtgg gattgaccgc ggagggcaag gtgctgcagc
     8281 tcggtctcaa aatcaaagac ggcgtggtcg acgtgatcgg cctcgacagc gtcctgaatc
     8341 gccccggcaa cgtggccgcc gaaggccgct gcacgatgcg caatctttct caggacgcgg
     8401 tggtgctccc gggtgtggat ttgctgaaga acaccaaggc gctcgccaag ttctgagccc
     8461 agagcttccg atgtggactc ggtcgccgaa ccccgcgacg tgttggctcg tgaacccgca
     8521 cctagtacgt gtgctgggtt cgtcgaccga ggtgtacctg agcacccagc ccgagcgaac
     8581 gttcgagctc actcgagata cattcgagtt cttggttgcg ttcgccagcg cacgcccgct
     8641 gcacgaagta ctatcctctc ccacgccgga tcagctgcag accgtagcgg ggctcgtgga
     8701 gaatggcttg ctggtccccg ccgaggccaa gctcgcccaa cctactccgc cccgggcacg
     8761 ccccgtttcg ctgcgcttct gtggggcagc gcctctgatt tcgcggccag cttcgccgga
     8821 gaacgaggct tcgctcggag acgccgtggt cgtgctcggc gcgaaatacg acgcgggtac
     8881 gctcgaccgc taccacaagg gcgcaagtcg cgggccggac gcaattcgac aagagagcag
     8941 ccaatatcct ctgttccccg atctgcgcac gggccgcgtg ttgggtttcc cagacgtcga
     9001 agctcgccgc tatctcgggg cgggcattcg cctgctggat gcaggagatg tgcaagccgg
     9061 cggcggaacg cccaacacca cctggcacca aggcctgcgg gaggaactgc gcgggctccg
     9121 aaagcgccag gccaaggtgt tgctcctcgg gggtgatcac agcgtgagct tggcctcgat
     9181 acaggcgctc caaggttcgc ccctcggcgt tcttcaattc gatgcgcaca cggattttgg
     9241 tctcgtggag cacgaagccc acctgacgca cgcgaacgtg atgcgccacg ttcaccgcga
     9301 agcacatgtc gcccacctcg tgtgctgtgg cctgcgcggc ttccagcagc tgcctgaact
     9361 ggagggcgtc gagcgttatc ggagctgctc cgtgggcgcg ctacgccgca tgaccagtag
     9421 cgaactggat gaagtattga gaccggacct cccgtactac gtgacggtgg acatcgatgt
     9481 gttggatgcg ccgctcgtgc ccggcacggc cgtgcccgag ccggacggcc
//
LOCUS       DASTKU010000428         3786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_749089, whole genome shotgun sequence.
ACCESSION   DASTKU010000428 DASTKU010000000
VERSION     DASTKU010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749089"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VFQ61_14685"
     CDS             complement(<1..1048)
                     /locus_tag="VFQ61_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955757.1"
                     /translation="MARFRGQALTVALLLATAGIWWTVNRHFPLPHWLLWGVLASWLA
                     SALWLASCAAVGWLVVQRLARDLGWLERLVIAIPLGVLTFAWCTFLLGLAGGLGRAAF
                     FLLPALFLLTLATSTARRDLKRAYRHRRALFEHAPVLRSRLALAAIAFGTIGLLLTYV
                     PILTPENIQHDARWYHLPIAQQYSSSGRIEPFAEGWILGTYPHLASLLYCWALTAPVG
                     ILGRLLLCAHLEYLLFLYTLAAIPVTIRALLGQRAFAGASWSWAAFFLYPGILVYDSN
                     LSTGADHVAAIWAPAGLLLVLRLWTSADLRRRSLSRKAGLLGAVAGGAALTKYSAICA
                     AAPLLIGLVWCCIRP"
     gene            complement(1187..2050)
                     /gene="nth"
                     /locus_tag="VFQ61_14690"
     CDS             complement(1187..2050)
                     /gene="nth"
                     /locus_tag="VFQ61_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET9955758.1"
                     /translation="MASAKPGASRRIVSDDVVSKSPSAVHAKRKAELERGESALSKAS
                     ARSSVKAGGPEAKPNPRGASKALAPQAALAVFEKLRTHHADAHCELDHQNPFQLLVAT
                     VLSAQSTDVMVNKITPDLFRRFPTPESLAQAKYEDVEAALNRIGMFRQKAKNIIGLSK
                     GLVEKHGGEVPRTLAELIELPGVGRKTANVVLGVAFGAPEGVVVDTHVQRLSQRLGFT
                     RETEPPEIEQALMQLFPRADWEPLPHVLIFHGRRICSAIKPACAACPVQGDCPSAFHA
                     ENVGRKAPRVR"
     gene            complement(2166..>3786)
                     /locus_tag="VFQ61_14695"
     CDS             complement(2166..>3786)
                     /locus_tag="VFQ61_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HET9955759.1"
                     /translation="LKETLGYFIEHRREVVTRRSRFELREAEAQREIVEGLGMAVTDV
                     DIVIRTIRESKDPDQARERLMALPLKGLEAFVRRAGRPEAEIQAAAERPEYTLSERQA
                     RAILEMRLARLTGLEQDKLAAEYGELCEEIARLRAILANEGLLMDVIVRELEEIREKH
                     GEPRRTEIIESDAEIQIEDLIQEEDMVVTISHSGYIKRTSVSTYKAQKRGGKGNKGME
                     ARDDDFVNQLFIASTHSFVFFFSNAGKVYVKKVYEVPQAARNAKGRAIVNFIELEEGE
                     KIAAITPVRGFEAGLFVTTITRGGQIKKTEVTEYENYREKGIIGVKISEGDQLLTAVV
                     TDGNAEFLIATRRGQSIRFDEQQVRSMGRNTAGVKAIELDEGDQVVALARTEPEHDQV
                     LAVCERGYGKRTPLAEFRTQNRGGKGIILIDASDRNGPVVGLGLVKASDEIMLITDRG
                     QTIRIRVDEIRETGRNAQGVKLMSVGEDERVVAAEPIGESSVPEVVAEGAEELAETVG
                     VEETAAAVSPEDESSDAAEPSPEPGDGEGSE"
BASE COUNT          648 a         1241 c         1179 g          718 t
ORIGIN      
        1 gcgggcgaat acagcaccag actagcccga tcaggagcgg cgcggcggcg cagatcgcgg
       61 agtacttggt gagagcggcc ccgccggcca cggctccaag caacccagcc ttgcgtgaaa
      121 gcgagcgcct tcgcaaatct gcgctggtcc acagccggag caccaataag agccccgccg
      181 gcgcccagat ggcagccacg tgatcggctc ccgtagagag gttcgagtcg tagacgagga
      241 tgcccggata caagaaaaac gcggcccagg accatgacgc tcccgcgaag gcgcgctgcc
      301 cgaggagggc gcggatggtt accggaatcg cggcgagcgt atacagaaac agcagatact
      361 ccaggtgcgc gcacagcaag aggcgcccca gaattccaac tggcgcagtg agcgcccagc
      421 agtacaacaa gctcgcgagg tgggggtacg tgcccaagat ccagccttca gcgaaaggct
      481 cgatgcgtcc cgagctcgag tactgctgcg cgatggggag gtgataccaa cgcgcgtcgt
      541 gctgaatgtt ctccggcgtg aggattggca cgtaggtcag caagagccca atcgttccga
      601 aggcgatggc ggccaacgcc aatcgtgagc gcagaaccgg agcatgctcg aagagagcgc
      661 gacggtgacg ataggcccgt ttcagatcac gccgcgcggt actcgtggcg agcgtcagaa
      721 gaaatagcgc ggggagtagg aagaaggcag ctcggccgag ccctcccgcg agcccgagca
      781 gaaacgtgca ccaggcgaag gtcagcacgc cgagcgggat cgcgatcacc aaacgctcga
      841 gccatccgag atcgcgggcc aagcgctgca ctacgagcca gcctactgcc gcgcagcttg
      901 ccagccagag cgccgacgcg agccaactcg ccaagactcc ccagagcagc caatgcggca
      961 agggaaaatg gcggtttacg gtccaccaaa tccccgctgt tgccagcaac agggcgaccg
     1021 tgagcgcctg gcctctgaat cttgccatgc ggggtcggct tatagcgcat cgctccgatc
     1081 catcctatcg agtcggggcg ggtccctcgt cgttcgagtt ggcgattcga ttgctggatc
     1141 gattcgattc agccgtgcaa agtcagcgca ggtagctcag cgtgagctag cgcacgcgcg
     1201 gagccttgcg gcccacgttc tcggcgtgaa aagccgaggg gcaatcgcct tgaaccgggc
     1261 aagctgcaca cgcgggtttg atggcactgc agatgcggcg tccgtgaaaa atcagtacgt
     1321 gcggcagtgg ttcccagtct gcgcgcggaa acaattgcat cagcgcctgt tcgatctcgg
     1381 gtggctccgt ttcgcgggtg aagccgaggc gctggctcaa gcgctgcacg tgcgtatcca
     1441 ccaccacgcc ctctggagcg ccgaacgcga ccccgagcac gacgttcgct gttttccgac
     1501 caacccccgg tagctcgatc agctccgcca aagtacgcgg cacctcgcca ccgtgcttct
     1561 cgaccaaacc tttcgagagc ccgataatgt tcttggcctt ttggcggaac atcccgattc
     1621 gattgagggc ggcttcgacg tcttcgtact tggcttgggc caggctctcc ggggtaggga
     1681 agcgacggaa cagatcggga gtgatcttgt tcaccatcac gtcggtgctc tgggcagaca
     1741 agaccgtggc aaccaggagc tggaacgggt tctggtgatc gagctcgcaa tgcgcgtccg
     1801 catgatgcgt tctcagcttc tcgaacacgg ccagcgccgc ttgcggagcg agtgctttgg
     1861 acgcgccccg ggggttcggc ttcgcctcgg gaccgcccgc cttcacgctg ctccgagcgc
     1921 tcgctttgct gagcgcgctc tcgcctcgtt cgagttcggc tttacgcttc gcgtgtaccg
     1981 cgctcgggct cttgctcacc acgtcatcac tgacaatacg ccgcgaggct ccgggcttgg
     2041 cactcgccac ggtgcgactg cgtggcccgg cagtcttgct cgccgctcgg aggctgctcg
     2101 catcggggcg ccgtgctttc gtagcgcgcc ctgtcttacg cgctgctttc gcagcgcgtt
     2161 ctgctttact cgctgccttc gccatcaccg ggttcgggag agggttcggc tgcatcgctg
     2221 ctctcgtcct cggggctcac ggcggcggcc gtttcttcaa cgccgaccgt ttcagcgagc
     2281 tcctccgcgc cttcggctac cacttcgggc acgctgctct cgccaatcgg ctcggccgcg
     2341 acgacgcgtt cgtcttcgcc gacgctcatc agcttgacgc cctgcgcgtt gcgccccgtc
     2401 tcgcggattt cgtcgacccg aatacggatc gtctgaccgc ggtccgtaat cagcatgatc
     2461 tcgtccgagg ccttcaccaa gccgagacct acgaccggtc cgttgcgatc gctggcgtcg
     2521 atcagaatga tgcccttgcc gccgcggttc tgcgtgcgga actcggcgag cggagtgcgc
     2581 ttgccatagc cgcgttcgca caccgcgagc acctggtcgt gctcgggttc cgtgcgtgcc
     2641 agtgctacga cttggtcgcc ctcgtccagc tcgatcgctt tcacgccggc cgtgttacgc
     2701 cccatggagc gaacctgctg ctcgtcgaag cggatgctct gaccacggcg cgtcgcgatt
     2761 aggaactcgg cgttgccgtc cgtcaccacc gcggtcagca gctgatcccc ctcggagatc
     2821 ttcacgccga tgatgccctt ttctcggtaa ttctcgtact cggtgacctc ggtcttcttg
     2881 atctggccac cgcgggtgat ggtggtcacg aacaagccgg cttcgaagcc ccgcacgggc
     2941 gtgatggccg cgatcttctc gccctcttcg agctcgatga agttcacgat cgctcgcccc
     3001 ttggcgttgc gcgcggcctg cggcacctcg tacaccttct tcacgtacac cttgccggcg
     3061 ttcgagaaga agaacacgaa gctgtgagtg gaggcgatga acagctggtt gacgaagtca
     3121 tcgtcgcgcg cctccatgcc cttgttgccc ttcccgccgc gcttctgcgc cttgtaggtc
     3181 gagacggaag tgcgcttgat gtagccggag tgcgagatcg tcacgaccat gtcctcttct
     3241 tggatcaggt cctcgatttg gatctcggcg tcgctctcga tgatttcggt gcggcgcggc
     3301 tcaccgtgct tttctcgaat ttcttcgagt tcgcgcacga tcacatccat gagcaggccc
     3361 tcgttggcca gaatcgcgcg cagccgagcg atctcctcac acagctcgcc gtactccgcg
     3421 gccagcttgt cttgctcgag accggtcaag cgcgccaagc gcatctccag gatggcgcgg
     3481 gcttgacgct cggacagcgt atattcgggc cgctctgcgg cggcttggat ctcggcttcc
     3541 ggccgacccg cgcggcgcac gaaagcttcc agccctttga gcggcagcgc catcaagcgt
     3601 tcgcgggctt ggtcgggatc tttggattcg cggatggtgc ggatcacgat atcgacgtcc
     3661 gtgaccgcca tgcccaagcc ttcgacgatc tcgcgctgcg cttcggcctc gcgcagctcg
     3721 aagcgcgaac ggcgcgtcac gacttcgcgg cgatgctcga tgaagtaccc gagcgtctct
     3781 ttcaga
//
LOCUS       DASTKU010000429         3152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1866233, whole genome shotgun sequence.
ACCESSION   DASTKU010000429 DASTKU010000000
VERSION     DASTKU010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866233"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VFQ61_14700"
     CDS             <1..1142
                     /locus_tag="VFQ61_14700"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET9955760.1"
                     /translation="DAFGAARRARRRGLLSLARAQDSAPLPEAWPSAMAEYLRTRDAV
                     EHSLERSNLCPLSERTAVEAELARLQARAAKAADELVRLSRGQLGAAESELREPGTGE
                     ALLLWMESNDGWHGFLQFEQSVEHFHTEPRPSGKAWLEPFESTLRRARRLSVLGLGEL
                     NREDVHSLSSAAPDRERLYVVYSLDLSPTSRHSEKERSVIVGDTLGDLPESSREVVRV
                     ARNWEAAGRDVITLLGADAQPARIRAALRDAKILHYAGHAHFEGPEGWGSYIPLQHAR
                     ITVADLLTWGEAPNEVVLSACDAALEPGLPHTAGLGIAQAFVLAGARAVVAPSRRISD
                     RAAAEFAESVSRRMSEGEDLTTAYHAVQATLARDSEARAFRLLVR"
     gene            complement(1330..2106)
                     /locus_tag="VFQ61_14705"
     CDS             complement(1330..2106)
                     /locus_tag="VFQ61_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955761.1"
                     /translation="MFARRSHRSVFAHLVFGACLSLLACSATQETDAPAQDVTAQSQS
                     QELVTVTTPDGALYTFLDAGGEVGVLVEAPESARAKLQLPDEGGSLKAFYERISGRPA
                     PQVLLDALSREENARGLSSANQTRIEPTPAAQFSKGVSAPLQGDVGIASQALTAAEFQ
                     DKFCNSGYDFLYCWPNTSGNPWVQIYSDVMHGAISAIDCAVRFRYRYEADGWKTFVDR
                     YATPGKWYHYYTGTGTRHRRFEVIGNDEGCRMHFAVWGNY"
     gene            complement(2253..>3152)
                     /locus_tag="VFQ61_14710"
     CDS             complement(2253..>3152)
                     /locus_tag="VFQ61_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955762.1"
                     /translation="RTLLSSADTSRALSLAVPAALLVVFLGIVQVRQRAQRQAYWSAL
                     REPSVEPLIRCIERSMAMAKRLPDVDAFSAQARAMAYAFYERHADAVTALESVDWLAK
                     APLVQAVGVSADSLVTLFCERDPDRAAELCRRALILADVSPLLPGAKQTRQYHATCLA
                     VAEALRGAPSGDSIAALESGARERRYPPLQLLAHFGLAVSAEGQGELLRAGGHREFLK
                     QRAPHAVALHRSREDYAVASPVSPHPDAALRAPSLRREMTRSDPRAASGKQSALRALR
                     PLLLLWGALLVAFLLIWRLLSSS"
BASE COUNT          557 a          959 c         1051 g          585 t
ORIGIN      
        1 cggacgcctt cggggcggcg cggcgagcca ggcgtcgcgg acttttgagt ttggcgcggg
       61 cgcaagactc cgctccattg ccggaagcgt ggcccagcgc gatggccgag tacctgcgca
      121 cgcgcgacgc cgtggaacat agcctcgaac gctcgaatct ttgcccgttg agcgaacgta
      181 cggccgtgga agcggaactg gctcggctcc aagctcgagc cgccaaagcg gccgacgaac
      241 tcgtacggct cagtcgaggc cagcttggag ccgccgagtc cgagctccgg gagccgggga
      301 cgggcgaggc gctgctcttg tggatggaat cgaacgacgg ttggcacggc ttcttgcagt
      361 tcgagcaatc ggttgaacac tttcacaccg agccgcggcc atcgggcaag gcttggctcg
      421 agcccttcga gtccactttg cggagggcgc ggcggttgag tgtacttggc ctgggggagc
      481 tgaatcgcga agacgtgcat tcactatctt ccgcggcgcc cgaccgagaa cggctgtacg
      541 tggtctactc actggatcta tctccaacca gtagacactc cgagaaagag cggagcgtaa
      601 tcgtcggtga caccttgggt gatctgcccg agtcttcccg agaggtggtt cgcgtggcgc
      661 gtaattggga ggccgctgga cgcgacgtca tcacgctgct gggcgccgac gctcaaccgg
      721 cgcgcatccg cgcagcgctg cgcgacgcca agatactgca ctatgccggc cacgctcatt
      781 tcgaaggtcc agagggctgg ggaagttaca ttccactcca gcacgctcgc atcacggtcg
      841 cagacttatt gacctggggt gaagctccca acgaggtcgt tctaagtgcc tgcgatgcgg
      901 cgctcgaacc ggggctgccc cacacggcag gactcggtat cgctcaagcc ttcgtcttgg
      961 ctggagctcg ggccgtggtc gcgccctcac gacgcatctc cgatcgggct gcagccgagt
     1021 ttgccgagtc agtatctagg cggatgagcg agggagaaga cctcacgact gcctatcatg
     1081 ccgtccaggc cacgctcgcg cgcgattcgg aggcgcgagc atttcgtttg ctcgtgcgtt
     1141 gagggtgcgt gattgccgag agtaccgagt tctgccgtgt gcgggatcag cttctctcgt
     1201 atgttttcga tgcgggtagg gttgggcctg agctcccgcg cattcggcac gggacgcagg
     1261 agctcgagcc cgctcgtgac ggggtcgctg tcgctcgtcg tgcttgaggt ctgctaagcg
     1321 cggtgcaact cagtaattgc cccagaccgc gaagtgcatg cggcagcctt cgtcgttgcc
     1381 aattacctcg aagcggcggt ggcgtgtgcc cgtgcccgtg tagtagtggt accacttgcc
     1441 cggcgtcgcg tagcggtcca cgaaggtctt ccagccgtcc gcctcgtagc ggtatcgaaa
     1501 gcgtaccgcg cagtcgatgg ccgagatcgc gccgtgcatg acatcgctgt aaatctgaac
     1561 ccaagggttg cccgaggtat tgggccagca atacagaaaa tcgtagccgc tgttgcagaa
     1621 cttgtcttga aactcggcgg cggtgagggc ttggctcgcg attcccacgt cgccttgcaa
     1681 aggtgcactc acgcccttcg agaactgcgc ggctggcgtt ggctcaatgc gggtctggtt
     1741 ggcggaagag agcccacgtg cgttctcttc acggcttaga gcatccagca gcacttgcgg
     1801 cgcgggccgt cccgagatgc gctcgtagaa ggctttcagt gagccgcctt catcgggtag
     1861 ttgcagtttg gctctggcgg attcgggcgc ctcgactagc acaccgactt cgcctccggc
     1921 gtcgaggaag gtatacagag cgccgtcggg cgtcgtgacc gtcacgagct cctgggattg
     1981 gctttgcgcc gtgacgtctt gagcgggtgc gtctgtttct tgggtggcgg agcaggccag
     2041 cagcgagagg catgctccga aaactagatg agcaaaaacg gatcgatggc ttcgtcgtgc
     2101 gaacatatcg aattctccca gtgagtggat tgggcgcgac caggtcgcac tgggttgttc
     2161 gcggctcgcc cgccgaatct ctcgctcttt ttggaatgag ctcgaatgtc tcgagcaagc
     2221 tcgttccccg ggctcgctcg gcagttgccc cactagctcg aactgagtag ccgccaaatc
     2281 agcaagaagg cgacgaggag cgcgccccag agtagcaaca gcggtcgcag cgcacgcaac
     2341 gcagactgtt tgcccgatgc ggctcgtgga tcgctgcgag tcatctcccg gcgtagactc
     2401 ggagcgcgga gcgcggcgtc agggtgcggg gagacgggcg aagcgaccgc atagtcctct
     2461 cggcttctgt gaagggccac ggcgtgcggt gcccgttgct tcaagaactc gcgatggccg
     2521 ccggctcgca acaactctcc ctggccttcc gcgcttacgg ccaagccgaa gtgagcgagc
     2581 agctgcaggg gaggatatcg cctctcgcgt gcgccgctct ccagcgcggc aatggaatcg
     2641 ccagacggcg cgccgcgcaa ggcctcggca acggcgaggc acgtggcgtg gtactgccgg
     2701 gtctgtttcg cgccaggtaa gagcggactc acgtctgcca agatcaatgc tcgtcggcac
     2761 agctctgcgg cgcgatccgg gtctcgctcg cagaacaacg taactaggct atcggcggag
     2821 acgccgacgg cctgaacgag cggggccttg gccagccaat ccacggactc gagcgccgtc
     2881 acggcgtcag cgtgtcgctc gtagaaggcg taagccatgg cccgggcttg cgcactgaag
     2941 gcgtccacgt ccggaagccg cttggccatc gccatcgatc gctcgatgca acgaatcaga
     3001 ggctcgacgc tgggttcacg caaagcggac cagtaggcct gcctttgtgc cctttgtcgc
     3061 acctggacga tacctagaaa aacaacgagc agcgccgccg gcacggccag ggacaacgct
     3121 cgcgaggtgt ccgcggaact caatagggtg cg
//
LOCUS       DASTKU010000430         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_527178, whole genome shotgun sequence.
ACCESSION   DASTKU010000430 DASTKU010000000
VERSION     DASTKU010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527178"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VFQ61_14715"
     CDS             <1..680
                     /locus_tag="VFQ61_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9955763.1"
                     /translation="AMVGFGDGEWRHKDVWKHTKQVVVQTSPRVELRYAALFHDIGKV
                     KTRSISPDGEVHFFGHAEVGARMFDKLERRERLFGADPALLEEVRFLVLHHLRASQYD
                     GQWTESAVRRFAREMGPYLEDLLCLSRADITTKRPEKKRKIIGLIDELSTRITDLAAL
                     DAREPPLPTGVGNALMTAFNLPPSRKIGEIKRALEACVESGELPARLESEAYVQFVAE
                     HRERFGL"
     gene            775..>2255
                     /locus_tag="VFQ61_14720"
     CDS             775..>2255
                     /locus_tag="VFQ61_14720"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9955764.1"
                     /translation="MHQPGEPTIKPVFLLAGLVKTAHEFGKFARLCGLSGCLVAAGAC
                     SSNTDSGTVPSFVRGGTSGVGGRTAQVGGSQQGGASGAGNGGASTLTQTGGASGATVG
                     SGGATQPPYVEPSETLDLNAVPSSVPTVRLDVDPAAIATLEQNPFDAADVTGTFTDSS
                     GVRYEGIQVNYRGAYQLQNLIRGEGSQRNWKLKFAKAQMYRARREWNFNYEPHIRQEL
                     AYDLMRFAGLKVPSARHVVLMVNGQRSGLFLEYEDPDNKDFLQDKFADDSGDLYKAST
                     DIPGQTPYFGTTEYLGDQDSAYLQRYQKKTNNDKAPEDYAVLRAFLQGLNSTPESELE
                     AWVTQHFDAQKFVRYLAVDNFISGWDGMPQRPKNYWLYEVRAAGRWVFIPWDMDATFQ
                     VRTTRLNPMGTNASIFYQLDAFESYQLSMNEGQERPLVRRLLKIPAIRTAYLQAYKTA
                     LTTYLEKTYLLNRISRLQALVEAQASADELMELQDARSDMQQF"
BASE COUNT          455 a          746 c          667 g          387 t
ORIGIN      
        1 aagccatggt cggcttcggg gacggcgagt ggcgccacaa ggacgtttgg aaacacacca
       61 aacaagtggt cgtgcagacc tcgccccgcg tggagctgcg ttacgccgcg ctgttccatg
      121 acatcggcaa ggtgaagacc cggagcatca gccccgacgg tgaagtgcat ttctttggcc
      181 acgccgaagt cggagctcgc atgttcgaca aactcgagcg ccgtgagcga ctgttcggcg
      241 ccgaccccgc gttgctcgag gaggtgcggt tcttggtgct gcaccacctg cgcgcgagcc
      301 aatacgacgg ccaatggacc gagagcgccg tgcggcgttt cgcgcgggag atgggcccct
      361 acctcgagga cctgctgtgc ctgtcgcgag ccgacatcac caccaagcgc cccgaaaaga
      421 agcgcaagat cattggtctg attgacgagc tctcgacgcg catcaccgat ctcgccgcgc
      481 tcgacgcgcg tgaaccgcct ttacccaccg gggtcggtaa cgccttgatg acggccttca
      541 acctgccgcc gtcgcgtaag atcggcgaga tcaagcgcgc cctcgaggcg tgtgtggaat
      601 cgggcgagct ccccgcgcgc ctggagtccg aagcctacgt tcagttcgtt gccgagcacc
      661 gcgaacgttt cggtctctaa caatccaaca acctgctgag ttagcgcatg ctcgacgttg
      721 cgcgtgcgca cttcacgcgc cacgctgcgc gttcaccgat cgaaggttgc gtgaatgcat
      781 cagccgggcg agcccacaat taagcctgtt ttcttactcg cgggactcgt aaaaaccgct
      841 cacgaattcg gcaaatttgc ccgcctttgc ggcctctctg gctgcctcgt tgcggccggt
      901 gcctgcagct ccaacacgga ttcgggaacc gttcccagct tcgtgcgcgg cgggaccagt
      961 ggcgtcggtg gtcgcaccgc tcaagtcggt gggagccagc aaggtggggc atcgggcgcc
     1021 ggcaacggag gtgcatcgac gctcacccaa acgggtggtg cgtcgggcgc cacggtcggg
     1081 tccgggggcg cgacgcagcc cccctatgtg gagccgagcg aaacgctcga cctgaacgcg
     1141 gttccaagca gcgtgccgac cgtgcgcctg gacgtggacc ccgccgccat tgcaaccctg
     1201 gagcagaacc cgttcgacgc cgccgacgtc accggcacct tcacggactc gagcggggtt
     1261 cgttacgagg gcatccaggt caactatcgt ggcgcctatc agcttcaaaa tctaattcgc
     1321 ggcgagggct cgcaacgcaa ctggaagctg aagttcgcca aggctcagat gtaccgcgcg
     1381 cggcgcgagt ggaacttcaa ctacgagccg cacatccgcc aggaactcgc ctacgacttg
     1441 atgcgctttg cgggcctcaa ggtgccctcg gctcgccacg tggtcttgat ggtgaacggc
     1501 cagcgttcgg gcttgttcct cgagtacgaa gacccggaca acaaagactt cctgcaagac
     1561 aaattcgcgg acgactccgg cgatctgtac aaggccagca cggacatccc gggccaaaca
     1621 ccgtatttcg ggaccaccga gtacctcggt gatcaagact cggcctacct gcagcgctac
     1681 caaaagaaaa ccaacaacga caaggcgccg gaagactacg cggtgctgcg cgcgttcttg
     1741 cagggcctga acagcacccc cgagagcgag ctcgaagcct gggtcactca gcacttcgac
     1801 gcgcagaaat tcgtgcgtta tctcgccgtc gacaacttca tttcgggttg ggacggcatg
     1861 ccgcagcgac ccaagaacta ttggttgtac gaagtccgcg cggccgggcg ctgggtgttc
     1921 atcccctggg atatggacgc caccttccaa gtccgcacca cccgcctgaa cccgatgggc
     1981 accaacgcat cgatcttcta ccaactcgat gccttcgaat cgtatcaact cagcatgaac
     2041 gaagggcaag aacgccctct cgtccgccgc ctgctcaaga tcccggctat ccgcacggcc
     2101 tacctccagg cctacaagac cgcgctgacc acgtacctgg agaagaccta cctcctcaac
     2161 cgcatctcac ggctccaggc cctggtcgaa gctcaagcta gcgccgacga gctcatggaa
     2221 ctgcaagacg cacgcagcga tatgcagcag ttcat
//
LOCUS       DASTKU010000431         5601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1420607, whole genome shotgun sequence.
ACCESSION   DASTKU010000431 DASTKU010000000
VERSION     DASTKU010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420607"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /locus_tag="VFQ61_14725"
     CDS             <1..1636
                     /locus_tag="VFQ61_14725"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9955765.1"
                     /translation="EPSCGNRLIEGSEQCDDGNTTSGDGCRSNCTIEKCGDGIKDGGE
                     QCDDANTTSGDGCSKLCLLETVCGNAVKEGLEQCDDGNNVSGDGCSSACQKEAVCGNA
                     VRESPEQCDDGNKLSGDGCDASCRVEGRCGDGVLGGAEQCDDGNITSGDGCRSNCSLE
                     SCGDAIKDPTESCDDGNTASGDGCSKLCQLETFCGNGAKEGTEECDDGNNVSGDGCSS
                     ACRIEPICGNSVKEGAEECDDGNKLSGDGCSSSCRIEAVCGDGKIGGAETCDDGNVLS
                     GDGCRADCSVERCGDRVVDAKEQCDDGNTVSGDGCSNTCTLETFCGNAKKEGVEECDD
                     GNNLSGDGCSSACRTEAVCGNRVVEGTETCDDGNKQAGDGCSATCRLENVCGDGVIGG
                     AETCDDGNTVSGDGCRSNCQREICGDGVRDALEQCDDGNTVSGDGCSRSCKIEIFCGN
                     GRVDQATEQCDDGNNTSGDGCSSTCQKEAVCGDGKLSGNEQCDDGNKTSGDGCSATCR
                     LEQYCGDGHLDSGEACDDGNTSNGDGCSSNCQFEVR"
     gene            complement(1709..4672)
                     /locus_tag="VFQ61_14730"
     CDS             complement(1709..4672)
                     /locus_tag="VFQ61_14730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9955766.1"
                     /translation="MLTSYPGRLYDGEVLEQIGLESLEYLMQELARLLGPIELGACRM
                     GVFTWDIACRNADGPFTVQLPLAMDRPGRRGRSKAEVPRLNLEHTRYFRERGLRRFVV
                     EPREYLTLGGNVPAAIFGALPEHHPVLFGCGAAQIEVSEGQQAWRVRMGSATTAELLA
                     EMIAALVYHYEPELDGGTTLADVCVNDGDFVARRRPDGSFELRLLAARQRESGVPRNR
                     LLLYLIQWMAYEDWVIDGNLVGLPVLISNPAVTFHGVLRGLRYRYRDLGQDEADAERD
                     GRQWIFEFGRSREGRAYRPWVERFLDLDLPLQFGRDPREHWWRLIPLQTKLGLLELRA
                     RQAPNSAEVSSAGALRAFLERISREIGVRWPEHVEQPSINDLDREALGSLMSEVGIVD
                     GSRREVADALIQGWPYRNFEQVLRRAGDGRALRKLKGRIAFGHVVPSADQGTLKSLPG
                     YAKPPSTRLPSPRANTSPTNTQPASAMPVSAMSASEPSRPPKHLSLANPELYSVLPLA
                     KGLESVASSRFPSFEAYMDAALHDATWGYYSRHVVIGPGGHFETNPEALSPNYGRWVA
                     AGAFRCWSELCSRAKLDSSEIFPVVEFGAGNGRLARDFLDAVTHHASDPENPQSSAWR
                     RFADRMRYRIYEMSSSLRERQRELLGASVVVGEGDARHPLRTLNRDFPEGLRGFVVTN
                     EVPDAFGVHKVLLTSAGAFVALVVPRAAPALGESMPSPLAERLRRVDQELRNSFSWHG
                     HAEDLYMDHPTWSAVMAQLSESRVEARDTLLDRLWFEECYVPVRCVPELHSHVVTNAV
                     QYATALAAEDSGVVTYVNLHAERFIRELGSSLCAGSIVTIDYGDTTWNLIQGGRRGEF
                     PFRIYKDDPQEYAPRPNDPYSAPGTQDMTADVNFTALALAGQSAGLEILHFGPERDVS
                     GAELTEVLKAAADQPVFAKFLGNPVFKVLVLGAGVSNVFSGPLMSELPLVHSEQALPK
                     SRRSLIAEIVQALVA"
     gene            5082..>5601
                     /locus_tag="VFQ61_14735"
     CDS             5082..>5601
                     /locus_tag="VFQ61_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955767.1"
                     /translation="MIRIEHVTCVRMGSVALSLWATLACSAGSDLEPSERVGESGSLA
                     AVESAVCRNGAPAPPRSVLDRAHIAEVEIRTSGPDWEALRYEGKSLFDNFIDDRRKLD
                     PFPYRTYAAEVIVDGVRYSNTGLRKTGFLGNLSAIKPSLVVEFGLGPHTPTLGRLREM
                     VLNNDLEDASQVR"
BASE COUNT         1163 a         1730 c         1750 g          958 t
ORIGIN      
        1 ggaacccagc tgcggcaata ggctcatcga gggcagcgag caatgcgatg atggcaacac
       61 cacatccggt gatggctgtc gctcgaactg tacgatcgag aaatgtgggg atggtatcaa
      121 ggacggcggc gagcaatgcg acgacgccaa cacgacctcg ggtgacggct gcagcaagct
      181 gtgtctgctc gagaccgtgt gtggaaacgc cgtcaaagaa ggactcgagc aatgcgatga
      241 tggcaacaat gtctcgggcg atggctgttc ctccgcctgc cagaaagaag cggtttgcgg
      301 caacgcagtg cgcgagagcc cggagcagtg cgacgacggc aacaagctga gcggtgacgg
      361 ctgcgacgcg agctgccgag tggaaggtcg ctgcggcgat ggcgtgctcg ggggagccga
      421 gcaatgcgat gacggcaaca tcacgtccgg tgacggctgc cgcagcaact gcagcctcga
      481 gtcgtgcgga gatgccatca aggatcccac tgagtcttgc gacgatggca acaccgcctc
      541 cggcgacggt tgcagcaagt tgtgtcagct ggagaccttc tgcggtaacg gtgcgaagga
      601 aggaaccgag gagtgcgacg acggcaacaa cgtctcgggc gacggctgct catcggcctg
      661 cagaatcgag cctatttgcg gcaattccgt gaaagaaggc gcagaggagt gtgatgacgg
      721 taacaagctg tccggagacg gctgctcctc cagctgtcga atcgaagcgg tgtgcggcga
      781 cggcaagatc ggtggagcgg agacgtgcga cgacggaaac gtcctctccg gagacggctg
      841 ccgggcggac tgcagtgtcg aacgctgtgg cgaccgcgtc gtcgacgcca aggagcagtg
      901 cgacgacggc aacacggtgt ccggcgacgg ttgcagcaac acctgcaccc tggaaacgtt
      961 ctgtggcaat gccaagaaag agggcgtcga ggagtgcgac gatggcaaca atctatccgg
     1021 tgacggctgc tcctcggcct gccgaaccga agccgtgtgc ggcaaccgcg tcgtagaagg
     1081 cactgaaacc tgtgacgacg gcaacaaaca agcaggcgac ggctgcagcg caacgtgcag
     1141 gctcgagaac gtctgcggag atggcgtgat cggcggggcg gaaacctgcg atgacggcaa
     1201 caccgtatcc ggggatggct gccgttcgaa ctgccagcgc gagatatgtg gagacggcgt
     1261 ccgcgacgcg ctcgaacagt gtgacgacgg caacaccgtg tctggtgacg gctgcagtcg
     1321 aagctgcaag atcgagatat tctgcggaaa tggtcgagtc gatcaggcga ccgagcaatg
     1381 cgacgacggc aacaacactt ccggggacgg ctgctcctcg acctgtcaga aagaggccgt
     1441 gtgcggcgac ggaaagctga gcggcaacga gcagtgtgac gacggcaaca agaccagcgg
     1501 tgacgggtgc agtgccacgt gtcgcctcga gcaatattgt ggtgacggac acctcgatag
     1561 cggcgaggcg tgtgacgacg gcaacaccag caacggcgac ggatgtagct cgaactgcca
     1621 attcgaggtc cgctagttta catggccgct agtttacatg gccgctagtt tacatggccg
     1681 agcggtccat gtcccccgac cgctcgcctc acgccactag cgcctgcacg atctccgcga
     1741 tcaaactgcg ccgtgacttc ggaagcgcct gctctgaatg gacgagaggc aactcggaca
     1801 tgagtgggcc cgaaaacacg ttgctcactc cggccccgag gaccagcacc ttgaacactg
     1861 ggtttccgag aaatttggcg aacacgggct ggtccgcggc ggccttcaaa acctccgtca
     1921 actccgcccc actaacgtct cgctcgggcc cgaaatgcaa gatctccaac ccggcacttt
     1981 gccccgccag ggccaaggcg gtgaagttga cgtcggcggt catgtcctga gttccagggg
     2041 cagagtacgg gtcgttcggg cgaggtgcat actcctgcgg atcgtccttg tagatccgaa
     2101 aaggaaactc gccgcgtcgc ccaccctgga tcaagttcca ggtcgtgtcc ccatagtcga
     2161 tggtcacgat gctgcccgcg cacagcgaag agccgagctc cctgatgaag cgctccgcgt
     2221 gcaggttgac ataggtcacc acaccagagt cctcggcggc gagtgcggtc gcgtactgaa
     2281 cggcgttggt cactacgtgc gagtgcagct cgggtacgca gcgcacgggt acgtagcact
     2341 cctcgaacca gagccgatcc agcagagtgt ctcgagcttc cacgcgggat tcggacagct
     2401 gggccattac cgcggaccaa gtcggatggt ccatgtaaag atcttcggcg tggccgtgcc
     2461 aactgaaact gtttcgcagt tcctgatcca cacggcgcag cctctccgcg agcgggctcg
     2521 gcatggactc cccgagggcc ggcgccgctc tgggtacgac caacgccacg aaagcgcccg
     2581 cactcgtgag taagactttg tgaacgccga atgcgtccgg aacctcgttg gtgaccacga
     2641 acccgcggag cccctcgggg aagtctcgat tcaacgtgcg gagtggatga cgcgcgtccc
     2701 cttcccccac caccacggag gctcccaaca gttcgcgctg ccgctctcgg agcgaactcg
     2761 acatctcgta gattcgatat cgcatacgat cggcaaatcg ccgccaagcg ctcgattggg
     2821 gattctcggg gtcacttgca tggtgcgtaa ccgcatccaa gaagtcgcgg gcgagccgtc
     2881 cgttgccggc cccgaactcg acgacgggga aaatctccga gctatcgagt ttggctctcg
     2941 agcacagctc cgaccagcaa cgaaaagcgc ccgccgcaac ccagcgaccg tagttcggtg
     3001 agagcgcttc ggggttcgtt tcgaagtgcc ccccgggccc aattacgacg tgccgactgt
     3061 agtagcccca agtcgcgtca tgcagagccg catccatata cgcttcgaag ctggggaatc
     3121 tactgcttgc cacggattct agacccttcg cgagcggcaa aacggaatag agctctgggt
     3181 ttgccaagct gaggtgcttt ggagggcggc tcggctcact cgcggacatt gcactcacgg
     3241 gcattgcact cgcgggctgc gtattcgtgg gagaggtgtt ggcgcgcggt gacggaagtc
     3301 tggtagacgg aggtttggca taacccggca aactcttcaa agttccctgg tctgcactcg
     3361 gaactacgtg accaaacgca atgcgtcctt ttagcttgcg tagagcacgc ccgtccccgg
     3421 cacgccgcaa gacttgctcg aagtttcgat aaggccaacc ttggattagc gcgtccgcga
     3481 cttcgcgacg cgaaccgtcg acgatgccca cctccgacat cagcgagccc agcgcctcac
     3541 gatccaagtc attgatggac ggctgctcca cgtgctcggg ccagcggacg ccgatttcgc
     3601 gcgagatgcg ttcgaggaat gcgcgcaaag cgccggccga gcttacttcc gctgagttgg
     3661 gcgcttggcg cgcgcgcagc tccaataacc cgagcttggt ttgcaaagga attagccgcc
     3721 accagtgttc ccgtggatcg cgtccgaatt gcaggggcag atcgagatcg agaaacctct
     3781 ccacccacgg ccggtaggca cgcccctcgc gtgagcgccc aaactcgaag atccattgtc
     3841 gtccatcgcg ctccgcgtcc gcttcgtctt gacccaagtc gcgataacga tagcgcagcc
     3901 cccgaagcac accgtggaac gtcacggccg gattcgagat cagcactggc agccctacca
     3961 agttgccgtc gatgacccaa tcctcgtagg ccatccactg aatcaaatag agcagcaagc
     4021 gatttcgagg aacgccggac tcgcgctgac gagcagcgag cagccgaagt tcgaacgatc
     4081 cgtccggtcg ccttctcgcc acgaagtcac cgtcgttgac gcaaacatct gcgagagtcg
     4141 tccccccatc gagctctggc tcgtaatgat aaacgagagc cgcgatcatt tcggcgagta
     4201 gctccgcggt cgtggcggaa cccattcgaa ccctccaagc ctgctgcccc tcggacacct
     4261 cgatttgcgc ggccccgcaa ccaaacagca ccgggtgatg ctcgggcagc gcgccgaaga
     4321 tcgcggccgg gacgttgcct cccaaggtca ggtactcgcg aggctcgacg acgaagcggc
     4381 gcaagccgcg ctctcgaaag tatcgagtat gctctaggtt caggcgtgga acctcggcct
     4441 tcgagcggcc gcggcgcccc ggccggtcca ttgccaatgg caactgcacg gtgaacggcc
     4501 cgtccgcatt gcgacacgca atatcccagg tgaatacgcc catgcgacag gcgcccagtt
     4561 cgattggtcc aaggagtcgc gcgagttcct gcatcaagta ctcgagcgac tcgagcccaa
     4621 tctgttccaa gacctcacca tcatagagcc gccccggata agacgtaagc aagctgtctc
     4681 gcgaccatcg gctggagagg aaagaagacg gcgccctcaa cccctcttcc cgcaggaact
     4741 cggcccaatc aacagtcgcg ctcgacccgg cgctctcgag ctcgatcctc gacggcccgc
     4801 ttcggggtga attttggccg agtccgcgcg gaggctccct catgtgagga agctttggcg
     4861 gcggcgcttg ctcgatgcaa cgccaatcag ctccgccccc taatcgagtc gcccgacttt
     4921 ggaaaaactt caagctgact tcagcggccg cgcgccgccc ggagctcgcg agcacgcggc
     4981 tcgcagggtt ggctcattct agcgcccgag ccgcccaagg cagattttgc tcgacgagcc
     5041 ccgcgcgctc gcgacatgga catggtcacc gaggagggaa catgatcaga atcgaacacg
     5101 tcacgtgtgt gcgaatgggg tcagtcgcgc tatcgctctg ggcaacgttg gcttgctccg
     5161 cggggtctga cctcgaacca agcgagcgag taggcgaatc cggctcactg gcggccgtcg
     5221 aatccgcagt gtgccgcaac ggggcgccgg cgccgcctcg atctgtcttg gatcgcgctc
     5281 acattgcgga ggtcgagatt cgtacgtcgg gaccggattg ggaggcgttg cggtacgagg
     5341 gcaagagcct tttcgacaac ttcatcgacg accggcgcaa gctcgacccg ttcccatatc
     5401 gaacctacgc cgcagaggtg atcgtcgacg gagttcgata ttcgaatacc gggctgcgca
     5461 aaaccggctt tcttggcaat ttgagtgcta tcaaaccgtc tctagtcgtc gagttcggac
     5521 ttggcccgca cactcccacc ctgggccggt tacgcgagat ggtcctgaac aacgatttgg
     5581 aggacgcttc ccaggtgcgt g
//
LOCUS       DASTKU010000432         4285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_303762, whole genome shotgun sequence.
ACCESSION   DASTKU010000432 DASTKU010000000
VERSION     DASTKU010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303762"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1253
                     /gene="galT"
                     /locus_tag="VFQ61_14740"
     CDS             234..1253
                     /gene="galT"
                     /locus_tag="VFQ61_14740"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET9955768.1"
                     /translation="MSTLRFDVTTGEWVAFSSVRSKRPDGFRSRAVHATIPPNSEPSC
                     PFCPGNEALTPPACEVEPDPRDPSRWSVRIFQNKFPVLEPVGTTTRRINGPLFREMDG
                     YGRHEVVVESPEHSRALFQQGVEHVARLLRVLHRRAATLYRDAGLEVVQIFKNHGSSA
                     GSSMRHPHFQIVGTPIVPRQMRIKYQTAAEYYQITGSSVYADLARAELVAQTRVIAQN
                     PEFVAFAPYASRAPFEMWVVPLRLAPTFESADPSTLPALAELLVDVLGRLSRALDNPP
                     FNLLLNSAPRRHADEPDFVWHIEIVPRLATAAGLELSTGMAINGVFPESAAELLRAAA
                     PTVTG"
     gene            complement(1304..1804)
                     /locus_tag="VFQ61_14745"
     CDS             complement(1304..1804)
                     /locus_tag="VFQ61_14745"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9955769.1"
                     /translation="MTMSSTTNRNDGTTLQLPSDNAILVSRTFAAPARVVFEAITRPE
                     HVRNWWAPRSRGEMTSCSIDLRVGGEWRFAMRANNGAEVEFYGTYREIQAPTRIVQTE
                     IFAPFPDVVSIVTVTLTEHGGRTTFESLSVYPSKEIRDQVIATGMEDGMRESYRQLTK
                     VVEGLA"
     gene            complement(1801..2133)
                     /locus_tag="VFQ61_14750"
     CDS             complement(1801..2133)
                     /locus_tag="VFQ61_14750"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9955770.1"
                     /translation="MLATLTALAEPNRLRILELLRTGPRPVGEISERLKMRQPQVSKH
                     LQHLKNAGLVVVHPLAQQRVYEVRAAPLAELHAWTEKFRELWADRLDALEDVIDSIQK
                     KETKRKRK"
     gene            2244..3734
                     /locus_tag="VFQ61_14755"
     CDS             2244..3734
                     /locus_tag="VFQ61_14755"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955771.1"
                     /translation="MYPAEVRATNVRGVRAPAFAIVVFVLGCGGRSSLGEFQEERTSG
                     GAASSASGGRHAGGTVSLSAGGLTAMGGQSALGGQSAMGGRAATGGRTASGGIVGSAG
                     AGGRSTPSTCDIDGVTYESSALNPANPCELCRPETSTGRWTPGSSGTCVEAISVGPMH
                     ACAIRGGRVHCWGDNESGELGDGTLVTRGSPAPVSGVPVRASAVSAGGAHSCAIANGA
                     VYCWGTNRFGQLGNGTTEPSMTSVLVSGLSAGVTSISVGLSFSCAVVEGAAVCWGTNQ
                     AGQLGNGSLVNSSVPIVVKGLEKDVSVISAGQEYACALQRGVAYCWGKNSSGQLGNGT
                     TTDSVVPVPVRALPLASQIETGEVDTCALVNGAAWCWGGNWYGQIDTNDQQTRTEPVH
                     IARVSGMASIAVGFEEVCAIESGGAMCWGNVITSVQAGNVLVGISAGPTRFGPDDGDI
                     TAISMSGENGCMIQRGRASCWGFNHHGQLGNNSRVASATPVLVEFP"
     gene            3889..>4285
                     /locus_tag="VFQ61_14760"
     CDS             3889..>4285
                     /locus_tag="VFQ61_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955772.1"
                     /translation="MRKPGRALSKVFVLLCVASCTELESARDEFGTSGRSGAGGAAGS
                     SAGASFGGIGANGGSAGVNGGSAGVNGGSASRGGADCAPKTEAGGGCSRCAGTDLVSL
                     ENACTTSGCTPFDNSARLTLVGADGRLPSL"
BASE COUNT          722 a         1273 c         1482 g          808 t
ORIGIN      
        1 ggggggcggc tcggcgtggc ctacttttcg gctcgaggcg tgccagcgcc cgctacgcag
       61 cggcgagctc tccccgcgag cgcgttggcc ttagccgcag gcgctcgcgc ggtgcttcgc
      121 gaagtgacgc gcgtcctcgg cgcgctcggg tcttctacgc taggctgtca gcgagtggag
      181 gagaccgcga ccgggcggtc ctcgatgcgg taccctggag cgaggaagaa ggcatgtcga
      241 cgctgcgttt cgatgtcacg accggtgagt gggtagcgtt ctcgagcgtc cgatcgaaac
      301 ggccggatgg ctttcggagc agagccgtcc atgccaccat cccaccgaac tccgaaccca
      361 gttgcccgtt ttgtccaggt aacgaggcgc tcactccgcc agcctgcgag gtcgagccgg
      421 acccgcggga tccttcgcgc tggagcgtcc gtatctttca aaacaagttc ccggtgttgg
      481 agccggtggg gacgactaca cgccgcatca atggcccctt gttccgtgag atggatggct
      541 acgggcggca cgaggtggtg gtcgagtctc ccgagcactc ccgcgctctc ttccaacagg
      601 gcgtcgaaca cgtagcgcgc ttgctccgtg ttttgcatcg acgagcggcg accctgtacc
      661 gtgacgccgg gctcgaggtc gtgcagattt tcaagaacca cggctcctct gccggttcgt
      721 cgatgcggca cccacacttc caaatcgtgg gcaccccgat cgtgccgcgc caaatgcgca
      781 tcaaatacca aactgcggcg gagtactacc aaatcacggg gagtagcgtg tacgccgatc
      841 tcgcgcgcgc ggagctcgtt gcgcagactc gcgtgattgc gcaaaaccca gaattcgtgg
      901 cgttcgcgcc gtatgcgtcg cgcgcgccgt tcgaaatgtg ggtggtgccg ctgcggctag
      961 ccccgacctt cgagagcgca gatccgagca cgttgccggc gctggcggag ctcttggtcg
     1021 acgtgctcgg tcggttgagc cgcgccctcg acaatcctcc attcaacttg ttgttgaaca
     1081 gcgccccgcg ccgtcacgcc gacgagcccg acttcgtttg gcacatcgag atcgttccga
     1141 ggctcgcgac cgcggcgggc ctggagctca gcactggcat ggccatcaac ggcgttttcc
     1201 cagaaagcgc ggccgagtta cttcgcgcgg ccgcaccgac cgtgacgggg taaacggata
     1261 cgaatcccgc aatcaacccg cgcgccgcag tgacacgccg agctcatgca aggccttcga
     1321 cgaccttggt gagctggcga tagctttcgc gcatgccgtc ctccattccg gtggcaatga
     1381 cctggtcgcg gatttctttc gatggatata cgctaagact ctcgaaggtc gttcgcccgc
     1441 cgtgctcggt gagtgtcacc gtgacgatgg agacgacatc gggaaacggc gcgaagatct
     1501 ccgtctgcac gatgcgcgtc ggagcttgaa tctcccgata ggtcccgtag aactcgacct
     1561 ctgcgccatt gtttgcgcgc atggcgaagc gccactcgcc cccgacgcgt agatcgatcg
     1621 aacacgaggt catctcgcct cggctccggg gcgcccacca gttacggacg tgctccggcc
     1681 gcgtgattgc ctcgaacacg acgcgtgcgg gcgcggcaaa ggtgcgcgac acgaggatcg
     1741 cattgtcgct ggggagttga agggtggttc cgtcgtttcg attcgtggtg gagctcatgg
     1801 tcatttgcgc tttctcttgg tttctttctt ttgaatgctg tcgatcacgt cctcgagcgc
     1861 atcgagccga tcggcccata gctcgcggaa tttttcggtc cacgcatgca gttcggcgag
     1921 cggcgctgcg cgcacctcgt agacgcgctg ctgcgccaag gggtgcacca ccaccagacc
     1981 agcgttcttc aggtgttgga ggtgtttgga gacctgcggt tggcgcattt tcagccgctc
     2041 gctgatctcg ccaacagggc gtggcccggt acgcagtagc tccaagatgc ggagccgatt
     2101 gggttcggcg agcgcagtga gcgtggcgag catgggcgta tattctcttc cgggtatgtt
     2161 cctgtcaagg aatattctcg atggggaaag taagtccgcg ggcccactgg cgaagtcggc
     2221 cgcttgcgag gtgggagttc cgggtgtatc cggcagaagt gagggcgacc aacgtgcggg
     2281 gagtcagagc gccggcgttt gcgatcgtcg tgttcgtgct tgggtgcggc gggcgaagct
     2341 cgctcgggga atttcaagag gagcgaacga gcggcggcgc agcgagctcc gcgagtggag
     2401 ggcgccatgc tggtggcacg gtgagtctat cggcgggtgg tcttaccgcg atgggcgggc
     2461 agagcgcttt gggcgggcag agtgccatgg gcgggcgcgc cgccaccgga gggcgtactg
     2521 ctagcggcgg catcgtcggg agcgcgggcg cgggcggacg ctcaacgcct tcgacttgcg
     2581 acatcgatgg agtcacttac gaatcctcgg cgctgaaccc ggcgaacccg tgcgagctct
     2641 gtcgaccgga gacgtcgacg ggtcggtgga cgccggggtc gagtggaacc tgcgtagagg
     2701 caatctcggt tgggccgatg catgcttgtg ccattcgcgg cggcagagtg cattgttggg
     2761 gcgacaacga aagtggcgag ctcggcgatg gcacgctggt cacgcgcggc tcgccggcac
     2821 cggtaagcgg agttccagtt cgagcttccg cagtttcggc gggaggcgcg cactcctgcg
     2881 caatcgcaaa cggcgctgtg tactgttggg gaacgaaccg ttttgggcag ctcggaaatg
     2941 gcacgaccga acccagcatg acctcggtgc tagtttcggg gctctcggcg ggcgtgactt
     3001 cgatatcggt tggcctgagc ttcagctgcg ccgtcgtcga aggagcggct gtttgctggg
     3061 ggacgaacca ggcagggcag ctcggcaatg gctcgctagt gaacagcagc gtccccatcg
     3121 tcgtgaaggg actcgagaag gacgtcagcg tgatttctgc gggtcaggaa tatgcttgcg
     3181 ccttgcaacg cggagttgcc tactgctggg gaaagaactc atcggggcaa ctcggaaacg
     3241 gcaccacgac cgacagcgta gtgccagtcc ccgtgcgggc gctcccgctc gcgtcgcaga
     3301 tcgaaaccgg tgaggttgat acttgcgcgc tcgtgaatgg cgcggcctgg tgttggggcg
     3361 gcaattggta cggtcaaatc gataccaacg accagcagac gcggacggaa ccggttcaca
     3421 tcgctcgagt gtcgggaatg gcttccattg cggtcggttt cgaggaggtc tgtgccatcg
     3481 agtcgggcgg ggcaatgtgt tggggcaacg tgatcacgag cgttcaagcc ggcaacgtgc
     3541 tcgtcgggat tagcgctggc cccacacgct tcggccccga tgacggagac atcacggcca
     3601 tttcgatgag cggagagaac ggctgcatga tccaacgcgg gcgcgcctct tgttgggggt
     3661 tcaaccacca tggtcaattg ggcaacaact ctcgcgtagc gagtgccaca ccggttctcg
     3721 tggaattccc gtagctaccc ccgcgggtta ctcctcgcgg gtgacgcttc gaaaccgacg
     3781 gcgcgacttt cgccgccaac gtgacgaact cgtgtcacgc acgggacatg ctcgagaccg
     3841 ctcgcagcgg cggtgcacgc ggctcgagct tctgttactt ggacgccgat gcggaagcct
     3901 ggccgcgctc tctcgaaggt gttcgtcctg ttgtgcgtcg cgagctgcac ggagctcgag
     3961 agcgctcgcg atgaattcgg tacctcgggt cgcagcggtg cgggcggcgc ggccggatcg
     4021 tctgctggcg cgagtttcgg gggaatcggg gcgaatggcg gaagcgcagg cgtgaatggc
     4081 ggaagcgcag gcgtgaatgg cggaagcgcg agtcgaggcg gcgcagactg cgctccgaag
     4141 acggaagcgg gaggcggctg tagccgttgc gctggcacgg acctagtaag cctcgagaat
     4201 gcgtgcacga cgagtggctg cacgccgttc gacaactctg cgcggctcac actggtcggc
     4261 gctgatggtc ggctgccatc actgc
//
LOCUS       DASTKU010000433        19446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_303791, whole genome shotgun sequence.
ACCESSION   DASTKU010000433 DASTKU010000000
VERSION     DASTKU010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 19446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19446
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303791"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            373..2703
                     /locus_tag="VFQ61_14765"
     CDS             373..2703
                     /locus_tag="VFQ61_14765"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET9955773.1"
                     /translation="MTIVPRGAVRVQPLPLPTRALRLASGIGIAPLDEQTLSGLTRAD
                     PGWIWSWAPPRHLLLDVATATTAVQAYRQEGGGSGQGVWIGVVDTGLDVSHPDLRTAS
                     GATRVRWWIDFTRLPAGLQPELESRFGCGAEAPCAIFSNHDIDILLNNGVSNDEPTDL
                     LGHGTHVAAVAASNGLALGKSYLGVAPEADLVVAKVTSYGEAILDPTVLRAVDFLFDR
                     AESAGAACVINLSLGSDMGAHDGSSALERGLSSYVGAQHPGRAIVVAAGNSGGVYSGV
                     TSAYPGPFGVHTEVHVPAMSPTRVPLVWQKPRKAGLTGAIDAYVAFRAPDRVRVGVER
                     NGQLLGDMVDVGRARSFGDAELNVLILNGVGAFEADSIAANTAIVTLDGTWPATDSLK
                     LRFEGNGTASVWVQGSGDLDPNKSIGVVVPFAQKEGTINVPASAPDLIAVGATTSRSN
                     WTSIDGTPVTLPSNSPLARAAFDSAAFFSSAGPSATGTIKPDIVAPGVNLIAAMSRSA
                     DPRLNESSVFSGQGLCEPVATCLITDEYHAVSSGTSVSTPLVTGAIALLFERRPSLTQ
                     NELRNLLQAGARRLSGGALNLAQVGPGGLDVVRTAQALELAASHGSSVPSDRSWLVFP
                     SDYVRPDLSWPSCGTAQLRDGDGNLADGFDELELRLEVSGGVLQAPLVRSAPGLYRFC
                     VASSAGHGGEDLMVHLWFGDEAIAHRVLRVSVDRGAAAGTATPRGGACGLGRPASPDA
                     LVFALWMGGLAAARRRRQTSVRLQEQTCDAGDETGR"
     gene            complement(2659..3906)
                     /locus_tag="VFQ61_14770"
     CDS             complement(2659..3906)
                     /locus_tag="VFQ61_14770"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HET9955774.1"
                     /translation="MSERWISALGALAMLGVAYAACPRDRRRAVNLRSVGGGLVLLLA
                     CAGLVLKTPVTGLFTLANSGVEKLLGFTRAGAEFVFGGLVSDTHTFGFIFAFQVLPTI
                     LFFSALMSVLYYLRVMPLIIETGGRLLERTLRVSGAESFSTVADVFVGQTEAPLAVRP
                     YIERMTRSELHACMVAGYATTAGGVLAAYVALLKDKVPGIAGHLIACSVMSAPAALVI
                     AKLLLPETETPETMGTSRIRLPKTSENVLDAVASGTTDGLKLAANVAAMLIVFLAFTA
                     LVDALLGGLGRVMGLELSLNRLLSYAFYPLAWLLGVPAGDTARLATLLGEKTLFNEFV
                     AYTHLAENLNRDPNWLSERGRLIASYALCGFANFGSIGVQIGGYVGLAPARRTDISSV
                     ALRAMFGGLLTTCLVACVAGLFL"
     gene            complement(3909..5252)
                     /gene="argG"
                     /locus_tag="VFQ61_14775"
     CDS             complement(3909..5252)
                     /gene="argG"
                     /locus_tag="VFQ61_14775"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET9955775.1"
                     /translation="MSRIYRSLPPEGTRLGIAFSGGLDTRCAVAWLARNGMKVYAYTA
                     DLAQPDEDDPAAIPDIALKHGAQAARLIDCRAALVREGLIAIQCGAFHLSSGGKKYFT
                     TTPLGRAVTTTAIIRAMREDDVHVFGDGSTHKGNDIQRFYRYGILTNPELKIYKPWLD
                     QKFVDAFGGRTEMSEYLNRIGLPHTMSVEKAYSTDSNLLGATHEAKDLERLDSGMRIV
                     EPIMGVAHYRSNVNIAAEEVSVEFVDGQPVAINGRRFDSLYELFVEANRIGGRHGLGM
                     SDQIENRVIDAKSRGIYEAPGMALLHIVWERLLSAIHNENTLDLYFTLGRRLGRVLYE
                     GKWFDPEAMMLKDALTRWLAPSVSGKVTLELRRGDDYTLVDTKAMYMAYGPDKLSMEK
                     VEDVAFTPADRIGALELQNLSVLDNRAFLAHHLDSVARLGSGQSVLPELLGKKEE"
     gene            complement(5337..7004)
                     /locus_tag="VFQ61_14780"
     CDS             complement(5337..7004)
                     /locus_tag="VFQ61_14780"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9955776.1"
                     /translation="MRRSLSDLQSGEAFGRYELLAPVAKGGMSRVWAARLRGTRGFQK
                     LVAIKTLIAEGSEAETLENMLLAEASVASRIQHPNVAQYQDLGERDGVLYVVMEWIDG
                     EALTSLLKQCREAPMPLGMAAEIVRQACKGLHAAHELRDDAFEPLGLVHCDVSPHNLM
                     ISTSGAVKLIDFGIAKATHQAPMAQGKLIAGKLAFMAPEQARGEAVDRRADLFSLGIM
                     LYLMTTGRHPFASSSPAETLHRIVKNAKVPPPRSWLKDYPAELERVVMRALSHHKEQR
                     FTTALEFFTELAGAVPITAQEPEVGAYLNRLCAGPLARRRQLIQDALKQAAERDERTD
                     PKAPMLSVMPPPSASYDSGTLLRHGATTTAPAYADVVLAPPPAPRRRSTWKLALAMGG
                     SAALAVGALQIRASLGHGGSGAEGHGNPVAAASTSRAPVQASPLPVAQPLAAPPQAQA
                     AKEDSETARSSLDQKAVAASVPLGHPPPSPAVNAAAKGNAACAPGHPCKKPTPQTPES
                     QAAALEATALEALPSPAAEPPAPPAAAEPPPPPKESTHAQVVDQYGI"
     gene            complement(7316..7468)
                     /locus_tag="VFQ61_14785"
     CDS             complement(7316..7468)
                     /locus_tag="VFQ61_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955777.1"
                     /translation="MATVPGSAADFALPSGFGGMVFDSRDWAPIGLLSCNAVSCTQEQ
                     CSNEVC"
     gene            7467..7973
                     /locus_tag="VFQ61_14790"
     CDS             7467..7973
                     /locus_tag="VFQ61_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955778.1"
                     /translation="MPAPTSAPQARDQVFYRYETADGRVVIVDSLDQVPPGIRPRIEQ
                     LHGSSHDGSSIDSTQGWNWGKLHVDGGSFGLGFGSALFAIGALALLVRSKRLLGALFG
                     LGLAIAIGAVYLGWLRRTTAQPGAEGSAPFATPQDIVQDAKRAVERAKLRQAEQDRAI
                     EQIQKEAR"
     gene            8468..11569
                     /locus_tag="VFQ61_14795"
     CDS             8468..11569
                     /locus_tag="VFQ61_14795"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9955779.1"
                     /translation="MGPHSLLPLPSSDLDGDACSRPHGVCELRRKKIAVLFDYMAFLS
                     DVYESRLREALHAKSREYDVDLVFVYGRALDEPHPHCASSNAIFEHVHPEASDGVIVL
                     STCLAAHGGSASVARLMARFEGLPCCSIGVELPRVPSVLVDNQRGMASAVEHLISVHG
                     CRRLAFIAGSPGNAESEVRLNAYRSTLESHGIAHDPALVVHGNFVRHLGAAAMRQLIG
                     RGVEFDAVVAANDAMAFGAVEVLRELGRHVPRDVPMVGFDDLPLARLGNPTLTTVAQP
                     FEAIAATALEYVLNATARGTAVNTVLPTDFVVRRSCGCGVRVFPRLVEGNVPQREAPR
                     VLEEPRVLATIERLLRVRGADSQLQARELLGSLGTELRGTPGAFLNALEALLERARGD
                     NERYRALQNAISWLRLELASERRPELESLFCDAFALIALVNTTAQAQHDISLDETYLR
                     LLRSSEQVSAAFDLTSLKQALERTLPTTGMGTAFVSCYVEGDPSRLEPFVCLVDGKAV
                     DPGVDTFPASAFFPPNLPGFERRKSLLAFPLVFEAERLGLAVFEYDPSVKGYPVVRDQ
                     IATALRTVGLHQEVVYKTMQHERSVQERLATAKRMKSLSLLAGGVAHDLNNALGPLVA
                     LPDAILSDIRNLNLPESMVAEVCADVLTIQSASLRAAHTIKDLLTLGRQGTTARQPLD
                     LRRVIEQCATNDGSRFAWQSQRQVKFMLDMPAEPLVVKASEPHISRAISNLIRNAVEA
                     IASVGQVTVRGELRVIDEPTTRYETIEPGEYVVITIEDTGSGIQNVDLGRVFEPYFSK
                     KPLADSSGSGLGLAIVHGVVKEHDGFIDVTSVLGSGTSFMLYFPRAAGEPATPTPRRE
                     SHTRAARILMVDDDAIQLRTGQRVLGRLGYEITSVKSSQVAYEAATQNPARPFDLLIL
                     DMLLSEGEDGLELFERIQRVLPHQRAIVVSGHAPNERAEQAINKGLLWLSKPYTADAL
                     ADVVESALNGRGVPLVSSLDRTSSTESSNASATPLHEPSSPSSLNQSLPRALQFPPRP
                     PVL"
     gene            complement(11645..12559)
                     /locus_tag="VFQ61_14800"
     CDS             complement(11645..12559)
                     /locus_tag="VFQ61_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955780.1"
                     /translation="MNTSAVETGIRAYARAVGAQAVRILAAGALASSILCGCSEKATQ
                     RTLPSVQVALDPSMAPIYEDGELTLYEARIGVPLPILAPTESQRSALQGTAAPPFPNA
                     PWLTLDDARLQLSWVLTNLDDEPHAVEVLIDPWNEFGRYFPGLQLVNAEEGEYLPNLS
                     GIDYYYSLEATSTGAASRRSGVFTFDDLDEMARDFATVMNLIQNPPPADDDGDSPTLT
                     YVNHAFAFQNHSDRDLLVKRWLPSTIPGLVGFDVGLRSTRPVKVAIEVVAEVLDTGSG
                     KVKTQGRSGAVLEAPTEIITAGAASAGQ"
     gene            complement(12559..13965)
                     /locus_tag="VFQ61_14805"
     CDS             complement(12559..13965)
                     /locus_tag="VFQ61_14805"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9955781.1"
                     /translation="MNQLSSSRASSAARAIVLRTAASWGTTVLATRVLDSGQSLRVGE
                     GEGALIAKPEGSPVADSPIRAVGNGWELDARGATGGELHLRGRTENPALLARSGAPIP
                     IVPGDWGLLQYGSFSVFFQFSEATPALQKRGRINWAVLLSFVFSCLAVGGGLLLIWAI
                     TTPPLIPKPLELTTDAELAAKFNLKPEQLALEAPSEKADTPATGEKDPGAKDVKAQGG
                     GRKVQHTEGKLGRNEKAPKTEQVGDPNTGLGGIAEVLSSDVGDEVKKTLNTISSVADA
                     LGGLRSQNVVLGRGSGMGLHGSGPGGGGVGPDGVPFGAGTLDTGFGAGRGGGYGTGAA
                     GPGGRGLGGNGRGGLGTGEGDGGGERKLASKDAAKPGQGLSPGQIQRVVMSRMGAFQA
                     CYEIAAAREPTLKGGVTVSFSIGPGGSVSQVSVSNSSLSNPRVEGCITRQFGRLQFPT
                     ADKPTNAAFPFVFRPSKR"
     gene            complement(13962..14498)
                     /locus_tag="VFQ61_14810"
     CDS             complement(13962..14498)
                     /locus_tag="VFQ61_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955782.1"
                     /translation="MRVRRLIVSGVVSLSVVFAAHQPEAHAEETGKAAKPGTKPVKRP
                     RDGKSVRATGKAGAEGKSASEASPTRAESLETAPAEARGAERDLPSRSSGPKDSETKA
                     GNGTASSASSATVKEAEGPEGVKTYQFGAIEVEGRLKSPQLIYFLRRVRAEFRAGALG
                     HRSFLGELSDTRREAAFR"
     gene            14806..15117
                     /gene="rplU"
                     /locus_tag="VFQ61_14815"
     CDS             14806..15117
                     /gene="rplU"
                     /locus_tag="VFQ61_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET9955783.1"
                     /translation="MAYAVIRTGGKQYRVAEGEVVRVATLAGNPGEKVQFNDVLLLSG
                     DTVQVGKPALSGASVEAEIVEHGRGEKLVVFKFRKRKRSRRKAGHRQDFTAVRITGVK
                     A"
     gene            15121..15402
                     /gene="rpmA"
                     /locus_tag="VFQ61_14820"
     CDS             15121..15402
                     /gene="rpmA"
                     /locus_tag="VFQ61_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET9955784.1"
                     /translation="MAHKKGGGSTRNGRGSNAQYRGVKVFGGEHVRAGGILVRQVGQT
                     IKPGRNVGVGRDYTLFALVDGTVTYEHDTKTKKRVSVYPVSAEQAVAAT"
     gene            15426..16238
                     /locus_tag="VFQ61_14825"
     CDS             15426..16238
                     /locus_tag="VFQ61_14825"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET9955785.1"
                     /translation="MDGTLVGAEGRIAPRDAEAIRELQRRSIPVTLCTGRMYSGTSAV
                     ADLLSLREPVACVEGSQIVEHPTGRHLWSAPLPSSHVAPLFEALNEHGPAAFVFARDR
                     VFFDGDGAHYLHYVTGWSQQAERIDAVVDVQQWEGDVTTLALVALGQQVQIEAVHGVI
                     QREHREHLQSACFPLARATMDGVWGLLVRSARVDKGTALEWLAGHYGVTTDELVAVGD
                     WLNDVPMLERAGRSFAMNQAPEVVKSAADEVLVADIWTGGGIAEAAERAGLL"
     gene            16279..18315
                     /gene="mutL"
                     /locus_tag="VFQ61_14830"
     CDS             16279..18315
                     /gene="mutL"
                     /locus_tag="VFQ61_14830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET9955786.1"
                     /translation="MSAVAPKVAVLPPELASQIAAGEVAERPASVVKELVENSLDAEA
                     TRLDVEIEGGGILRICVTDDGFGMSEADARLALERHATSKLRSLDDLMNVRSYGFRGE
                     ALPSIASVSRFRLCTRERDAELGIELFVEAGATPVARQIGARAGTRIEIADLFFNVPA
                     RRKFLRSSATESGHVADVIENAALARPDVTFTLSRDGRRVRELLRVTTRAERVAQVLD
                     TRDLARCEGERGPLAVEAYLSRPEAARAGGAGLHLFVNGRNVRDRALLATIAHAYGSV
                     LEKGRYPRGVVYLDIAPELVDVNVHPQKSEVRFADPRAVSDVVYALVSRNLASAFSLP
                     PQATHHRSLSERPLPGASPDREALRSSTPQSPSPEPERNTEPGWGAARSSPPVFPLRT
                     SEPASAARTVFTVSEPLHPLHDAAAPLRDAAEPLRDAAAMQLKTAELAPLEASSPDPS
                     APQVASISVGSTPSSRPASTSVALPPARPEPPEAESKWQNLRFVAQLRNTYLLCEGSA
                     GIYLLDQHAAAERVTFSRLRRQYAAREVGAQTLLFPVLVELTPPEAEVVEARASELAE
                     VGLDVRMRGSSSASLHSVPKLLARAHPERLLRDLLLEVTRAGERAFSDAVDLALATLA
                     CHGSARAGDELGPAEVRALLLGLDEADFAGHCPHGRPIVTFTAWAELERKVGRR"
     gene            18312..19238
                     /gene="miaA"
                     /locus_tag="VFQ61_14835"
     CDS             18312..19238
                     /gene="miaA"
                     /locus_tag="VFQ61_14835"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET9955787.1"
                     /translation="MNSRATPDLPELLVIVGPTASGKTDLAIQLAEQLNGEIISADSV
                     QVYRQFDIGTGKPSHEQLARVPHHMIGVVEPAEHLDAASFAEQALSTISEIRSRARTP
                     IVCGGTFLWVKSLIYGLAEGPKANPEIRARHRELVERSGRAALYAELLEKDPASAQRL
                     SPNDFVRVSRALEVLELTAIPMSRFHAQHGFAQARFRARLLGVQRTREELDERILART
                     RAMLEAGWIDEVRRLVELGFSECRAMGSVGYKQVFAALAQTGHPDPDELAISIYRATR
                     VFARRQRTWLRDQKVEWLASVSEFDRSISTVP"
BASE COUNT         3295 a         6212 c         6316 g         3623 t
ORIGIN      
        1 gagcgccgaa tgcccgggag gtttctgtga cccggtgctg tttgcttgcg tagaatgccg
       61 agattcgcgt gattgttcgc tggaagcgct atgtgagccc agcacccacc gctgccagcg
      121 cttttgagca cgagctgact ctcatagaat gagccgtgcg cgtggcggga taagctgtgc
      181 gcattgagat aagctcgagc cagtcccgtg cgtcggaact ttggcttgct gtgcggtgcc
      241 gttttcacct tgtcgaggct ggcttcagcc gaggtcgacc tagcgcgggc tctgcacgct
      301 ctgcaatcgg tcagtgccac cgagcggacg agagcgcgcc gattgctctt gcgaggagca
      361 gcgagtgcga gcgtgacaat cgtgccacgc ggcgcggtca gggtgcagcc gctcccctta
      421 cccactcgcg cgctgcgcct cgcatcgggc atagggattg cgccgctgga tgagcaaacc
      481 ctgtcgggtc tcactcgagc tgaccctggt tggatttgga gctgggcccc tccgcgccac
      541 ctgctgctcg acgtggctac ggccaccacg gccgtgcaag cctatcgtca ggaaggcggt
      601 ggatcggggc agggggtttg gattggggtt gtcgacaccg ggttggacgt gtcgcatccc
      661 gatctgcgta cggcgagcgg tgcgacccgc gtgcgttggt ggatcgactt cacgcggctt
      721 ccggctgggc ttcaaccgga gctcgaatcc cggtttggat gtggcgccga agccccctgc
      781 gcgatctttt cgaaccacga tatcgatatt ctgttgaata acggcgtgtc caacgacgag
      841 ccgacggacc tgctcggtca cggcactcac gtcgcggcgg tcgccgcgag caacggtctc
      901 gcactgggga aaagctacct cggcgtggct ccagaggccg atctggtcgt ggccaaggtg
      961 acctcctacg gcgaagcgat cttggatccc acggtgctac gcgccgtgga tttcctgttc
     1021 gatcgcgccg agagtgcagg cgccgcgtgc gtcatcaact tgagcttggg cagtgacatg
     1081 ggcgcgcacg acggcagctc ggcgctcgag cgcggcctgt ctagctatgt tggtgcacag
     1141 caccccggaa gagcgatcgt ggtcgcggcg ggcaatagcg gcggggtcta ttccggtgtt
     1201 acgagtgctt atcccggacc gttcggggtg cacaccgaag tgcacgtgcc cgcgatgtca
     1261 cccacgcgcg tgcccttggt ttggcaaaag ccgcgcaagg ccggcctgac cggcgcgatc
     1321 gatgcgtacg tcgcgtttcg agcccctgat cgcgtccgcg tgggcgtgga gcgcaatggc
     1381 cagctgctcg gagacatggt cgacgtcggt cgcgcgcgca gcttcggaga cgccgagctc
     1441 aacgtgctca ttctgaatgg tgtgggcgct ttcgaagcgg actcgatcgc agccaacacc
     1501 gcgattgtca cgctcgacgg tacctggccg gccacggatt cactcaaact ccgcttcgaa
     1561 ggcaacggca ccgcgtcggt atgggtgcaa ggtagtggtg atctcgaccc caacaagagc
     1621 atcggcgtgg tcgtgccgtt cgcgcaaaaa gaaggcacca tcaacgtgcc ggcgtccgcg
     1681 cccgacctga tcgcggtagg ggcgacgacg agccgtagca actggacgtc gattgacggc
     1741 acacccgtca cgctgccgtc caacagcccg ctcgctcgag ccgccttcga cagcgcggct
     1801 ttcttcagtt cggcggggcc gtccgccacg ggcaccatca agccggatat cgtggcgccg
     1861 ggcgtgaacc tgattgcggc gatgtcgcgc tcggctgacc cgcgtctgaa cgagagcagc
     1921 gtgttctcgg ggcagggcct gtgtgagccc gtggccacgt gcctgataac cgatgaatat
     1981 cacgccgtga gcagcggaac ctccgtttca acgccgctgg tgaccggcgc gattgctctg
     2041 ttgttcgagc gacgcccctc tctcactcaa aacgagctgc gcaacttgct gcaggcggga
     2101 gcgcgtcgct tgagtggagg ggcactcaac ttggcgcaag taggaccggg cggtttggat
     2161 gtcgttcgaa cggcgcaggc gttggagctg gccgcgtccc acggttcttc agttccttcg
     2221 gaccgcagtt ggctcgtctt tccatcggac tacgtgcgtc ccgatctctc ctggccatcg
     2281 tgtggcacgg ctcaactgcg cgacggggat ggcaatctgg ccgatggctt cgatgaactc
     2341 gagctccggc tggaggtcag cggcggtgtt ctgcaagctc cgctcgtacg gagcgctccc
     2401 ggcttgtatc gcttctgtgt cgcctcgagc gccggtcacg gaggtgaaga cctcatggtg
     2461 cacctttggt ttggagacga ggcaattgcg catcgggtgc tgcgcgttag cgtggatcgc
     2521 ggtgccgccg caggaacggc tacaccgcgc ggtggcgcgt gcggcttggg gagacccgcg
     2581 tcacctgatg ccctcgtgtt cgctctgtgg atgggtgggc tggcggccgc gcgccgtcgg
     2641 cgccagacga gcgttcgcct acaagaacaa acctgcgacg caggcgacga gacaggtcgt
     2701 tagcagccct ccgaacatgg cccgcagggc cacgctcgag atatccgtgc ggcgcgcggg
     2761 agcgagcccg acataacccc cgatttgtac gccgatgcta ccgaaattgg cgaagccgca
     2821 cagcgcgtaa ctcgcgatca agcgcccgcg ttcactgagc caattcgggt cccgatttag
     2881 gttctccgcc agatgggtat aggccacgaa ctcgttgaac agagtcttct caccgagcag
     2941 cgtcgcgagc cgggcggtgt cgcccgcggg cacccccagc aaccaggcga gggggtaaaa
     3001 tgcgtacgag agtaggcgat tcaggctcaa ttcgagcccc atcacgcgcc ctaggccacc
     3061 caacagcgcg tcgaccaaag ccgtaaatgc caggaacacg atcagcatcg cggccacgtt
     3121 ggctgcaagc ttcaggccat ccgtcgtccc cgatgcaacg gcgtcgagca cgttttccga
     3181 cgtcttgggc aagcgaatgc ggctcgtgcc catggtctcc ggtgtctctg tttcgggcag
     3241 cagcagcttg gcgatcacca acgccgccgg cgcgctcatc acactgcacg cgatcaggtg
     3301 tccggcgata cctggcactt tgtccttcag cagcgccacg taagcggcga gcacgccccc
     3361 ggcggtagtg gcgtagcctg ccaccatgca ggcgtgcagc tccgagcgtg tcatgcgctc
     3421 gatgtaaggg cgaaccgcga gcggcgcttc cgtctgtccc acgaacacgt cggctaccgt
     3481 cgagaagctc tccgccccac tcacgcgcag cgtgcgctcg agtagccggc cccccgtctc
     3541 gatgatcaag ggcatcaccc ttaggtaata tagtacgctc atcagcgcgg agaagaacaa
     3601 gatcgtgggc aaaacctgga acgcgaagat gaacccgaac gtgtgcgtgt cagaaaccaa
     3661 gcctccgaac acgaactccg cgcccgcgcg cgtgaatccg agcagcttct ccacgccgct
     3721 gttggccaac gtgaataggc ccgtaactgg cgtcttcaac accagcccgg cacacgcgag
     3781 tagcaagacc agccccccgc cgacgctgcg caggttgact gcgcggcgtc ggtcacgcgg
     3841 acacgcggcg tacgccacgc ccagcatggc gagcgcaccg agcgcgctga tccagcgctc
     3901 gctcatgctc actcttcttt tttgccgagc agctccggca gcacgctctg ccccgagccc
     3961 agtcgcgcca cgctgtccag gtggtgggcc aggaacgctc gattgtccag caccgatagg
     4021 ttctgcagct cgagcgcgcc gatgcggtcc gccggggtga aagccacgtc ctcgactttt
     4081 tccatcgaca gcttgtccgg tccgtaagcc atgtacatgg ctttggtgtc gaccagggtg
     4141 tagtcatcgc cacgccgcag ctcgagggtg accttgccgg acacgctcgg cgcgagccaa
     4201 cgcgtcagcg cgtctttgag catcatggct tctggatcga accatttgcc ttcgtacaac
     4261 acccggccca accggcggcc caaggtgaag tagagatcca gcgtgttctc gttgtggatg
     4321 gccgagagca ggcgctccca gacgatgtgg agcagcgcca tgcccggcgc ctcgtagatg
     4381 ccgcggctct tggcgtcgat cacgcgattc tcgatctgat cgctcatccc gagcccgtga
     4441 cggccgccga tgcgattggc ttccacgaac aactcataga gtgagtcgaa gcggcgcccg
     4501 ttgatcgcga cgggttgccc gtcgacgaac tctacgctga cttcctcggc cgcgatgttc
     4561 acattcgagc ggtagtgagc cacgcccatg atcggctcga cgatgcgcat gccgctgtcc
     4621 agacgctcga ggtctttggc ttcgtgcgtg gcgccgagca ggttcgagtc cgtgctatag
     4681 gccttctcta cgctcatcgt gtgcggcagg ccgatgcggt tgagatattc actcatctcg
     4741 gtgcggccgc cgaaagcgtc tacgaacttc tgatctagcc aaggcttgta gatcttgagt
     4801 tcgggattgg tcaggatgcc gtagcgatag aagcgctgga tgtcattgcc cttgtgcgtg
     4861 ctgccatcgc cgaacacgtg cacgtcgtcc tcgcgcatgg cacggatgat cgcggtggtg
     4921 gtgaccgcgc ggcccagcgg ggtggtggtg aagtacttct tcccgccgct cgagaggtgg
     4981 aacgcgccac attgaatggc gatcaagcct tcgcgcacca gcgccgctcg gcaatcgatc
     5041 aggcgcgccg cctgggcgcc gtgcttcagc gcgatgtcgg ggatggcggc cgggtcgtct
     5101 tcatccggct gagcgagatc cgcggtgtac gcatagacct tcatcccgtt gcgagcgagc
     5161 caagccaccg cacaacgagt gtcgagacca ccggagaacg caattccgag gcgggtgccc
     5221 tcgggaggca gagaacgata aatacggctc atggcggccc gagccgtagc acgaccgtgt
     5281 tgagggaaca cggtcgaagg ggactagggt ggcccctgaa gtgacgcggc ggcgcctcag
     5341 atcccgtact gatccaccac ttgcgcgtgc gtggactctt tggggggcgg cgggggttcc
     5401 gcggcggcag ggggggcggg aggctctgcc gctggggacg gcagtgcctc gagcgcggtg
     5461 gcctccaagg ccgcggcctg cgactcgggc gtctgcggcg tgggcttctt gcacgggtgg
     5521 cccggtgcgc acgcggcgtt cccttttgcg gcggcattta ccgcgggcga agggggcggg
     5581 tgaccaagcg gaaccgaggc agcaacggct ttctggtcca gtgacgaccg agccgtttcc
     5641 gagtcttctt ttgccgcttg ggcctgcggc ggagccgcca agggttgtgc gaccggcagc
     5701 ggcgaggcct gcaccggcgc gcgggacgtg ctcgcggcgg ccactgggtt gccgtgccct
     5761 tcagctccgg agccgccgtg cccgaggctt gcgcgaattt gtagggcgcc taccgccaag
     5821 gcggcgcttc cgcccatggc caacgcgagc ttccaagtcg agcgccgacg tggggccggg
     5881 ggcggagcca acacgacatc tgcgtaggcc ggcgccgtcg tcgtcgcgcc gtgacgcagc
     5941 aacgtccccg agtcgtacga ggcgctcggc ggcggcatca cgctcaacat cggagccttt
     6001 ggatcggttc gctcgtcccg ctccgcggct tgcttgagcg catcctggat caattgtcgg
     6061 cgccgcgcga gtgggcccgc gcacaagcga ttcaggtacg cgccaacctc cggctcctgg
     6121 gcggtaatgg ggaccgctcc ggccagctcc gtaaagaact cgagcgcggt agtgaagcgt
     6181 tgctctttgt ggtgcgacag cgcgcgcatc acgacacgct ctagctccgc ggggtagtcc
     6241 ttcagccagg atcggggcgg cggaaccttg gcattcttga cgatccggtg cagcgtctcg
     6301 gccggtgagc tgctggcgaa cgggtgccgt cccgtcgtca tcaaatagag catgatgccg
     6361 agcgaaaaca ggtcggcgcg gcgatcaacg gcttcgcccc gggcctgctc cggtgccatg
     6421 aaggccagct tgccagcgat caacttgcct tgagccatgg gcgcctgatg ggtggctttc
     6481 gcgatgccga aatcgatgag tttcaccgcg cccgatgtgc tgatcatcag gttatgcggg
     6541 gaaacgtcgc agtgcaccag cccgagtggt tcgaaggcgt cgtcgcgcag ttcgtgtgcc
     6601 gcgtgcagcc ctttgcaagc ttgacgcacg atctcggcgg ccatgccgag cggcatcgga
     6661 gcctcccggc actgcttgag tagagaggtc agcgcttcgc cgtcgatcca ctccatcacc
     6721 acgtacaaca cgccgtcgcg ctcacccaag tcttgatact gggctacgtt ggggtgctga
     6781 atgcgcgagg ccacactcgc ctcggcgagc agcatgtttt ccagcgtctc ggcctcgctg
     6841 ccctccgcga tcaaggtctt gatcgcgacc agcttttgaa agccgcgcgt gccgcgcaga
     6901 cgcgcggccc acacgcgaga catgccgcct ttggccaccg gcgcgagaag ctcgtaccgg
     6961 ccaaacgctt caccagactg cagatcagat agacttcgac gcatgattgt cgagagaggt
     7021 tgatcgagga gtcctcgagt gagagtgcgc cctacgagtt tttcgccctc gattctagct
     7081 ctgctccgcc tctcggcccc ttggaacgtc cacaaaggtg aggactttag ctgtaattag
     7141 cctgtccgta tcgtggcgcc gctcgccagg cggatttcca acatgcgctg ctcgcggccc
     7201 taaccaagtt gcgacgttcg tgttgcatcg gggcgtggct gcaggcaccg tggacgtgtg
     7261 ttgcgagacg ctcgtgacga tcgagcctcg agccggctcc gacaccgttc ttggactagc
     7321 agacctcatt cgaacactgt tcttgggtac aggagactgc gttgcaggag agcaagccaa
     7381 tcggagccca gtcgcgcgaa tcgaacacca tgccgccgaa accggatggc aacgcgaaat
     7441 ccgccgctga accgggaacc gttgccatgc cggcacccac atccgcaccg caagcgcgcg
     7501 atcaggtctt ctatcgatac gagaccgcag atggccgcgt ggtgatcgtc gactcgctcg
     7561 atcaagtgcc ccccggcatt cgaccacgga tcgagcaact acacggcagc tcgcacgacg
     7621 gcagctcgat cgactcaacg cagggttgga attggggcaa gctgcacgtc gacggcgggt
     7681 cttttgggct tggtttcggg agcgccttgt tcgcgatcgg agccttggct ctgctcgtga
     7741 gatccaagcg tctgctgggc gcgctgtttg ggctcgggct cgcgatcgcg attggcgctg
     7801 tttatctcgg ctggttgcga cgcacgacgg cgcagcccgg agcggagggc tccgctcctt
     7861 tcgcgacccc acaagacatc gtccaagacg ccaagcgagc ggtcgagcgc gccaagcttc
     7921 gccaagcgga gcaggatcgc gcgatcgagc agatccaaaa agaagctcgc tagtcagctg
     7981 ctgcgagtgg ctcgcgctcg gcgcggtgga gtgcttttcc aacccgtgac gatggtccgc
     8041 gtagagcgca cgatttcagc tcgatcgccg ccctgaaatc gtcgaaatgt cgagcgccat
     8101 tccgttgaca ttcatgtggg tgatttgcct gctacccgtt tgggtatatc cggttcacca
     8161 aatttttcgt gggagctttc ctcgggcggc tcaaaacgcg aaagcgatgt ttggcgtcca
     8221 gttgccccgc tcaccccaga ggtgaagcac ctccactcgg aacgctgtcg gcatgagcta
     8281 ggcccagcat gagcgagccg tgccgcgttg gcgctacatc tctcaggtta gacaaccgac
     8341 ctccagagtg ggattgcgtt gcgaggattt cgcgtcggag tcgacgcttg cggcgctgtt
     8401 ttgcgactgc tagcttcgac gtgtttcgcc gggtgtgttt cctgtccggc gagctgcgaa
     8461 acgcaacatg ggcccacact cgttgctgcc gctcccgtct tcagacttgg acggtgacgc
     8521 ctgttcgcgt ccgcacggag tgtgcgaact gcgccgcaag aagatcgccg tgttgttcga
     8581 ctacatggcg ttcttgtcgg atgtctacga gtcgcgcttg cgcgaggccc tgcacgcgaa
     8641 gagtcgcgag tacgacgtcg atctcgtgtt cgtatacggt cgagccctcg atgaaccgca
     8701 tcctcactgt gcgtcatcga acgcgatctt cgagcacgtg cacccggagg cgtcggacgg
     8761 ggtgatcgtc ctgtccacct gcttggcggc ccacggtgga agtgccagcg tggccagact
     8821 catggcgcgc ttcgagggct tgccatgctg cagtatcggc gtggagctcc cgcgcgtgcc
     8881 gagcgtgttg gtggacaacc aacggggtat ggcgagcgcg gtcgaacacc tgatctcggt
     8941 gcacggctgc cgtcgcttgg ctttcatcgc cggtagcccc ggcaacgccg agtccgaggt
     9001 gcgcctgaac gcctaccgtt cgaccctgga gagtcacggt atcgcgcatg atccggcgct
     9061 ggtcgtgcac ggcaacttcg tccggcactt gggcgccgcc gccatgcggc agctgatcgg
     9121 gcggggcgtc gagttcgacg ccgtggtggc cgccaatgac gccatggcgt tcggcgcggt
     9181 ggaggtcctg cgtgaattgg ggcgccacgt gccgcgtgat gtacccatgg tcggcttcga
     9241 tgacttgccg ctcgcgcgac tcggaaatcc gacgttgacc acggtagcgc agcccttcga
     9301 ggcgatcgcg gccaccgcgc tcgagtacgt gctgaacgcg acggcgcgag gtacggcggt
     9361 gaacaccgtg ctgcccacgg acttcgtggt gcgccgctcg tgcggctgtg gcgtgcgcgt
     9421 gttcccgcgc ttggtggagg gcaacgtgcc tcaacgcgag gctccgcgcg tgctcgaaga
     9481 gccacgcgtg ctcgcgacga tcgagaggtt gctgcgagta cgcggcgccg acagtcagct
     9541 ccaggctcgc gagttgctcg gctcgctagg gaccgagctg cggggcacgc ccggcgcatt
     9601 cttgaatgct ttggaagcgc tactagagcg cgcgcgcggg gacaacgagc gttaccgggc
     9661 gctgcagaac gccatctcgt ggcttcggct cgagctggcg tcagagcggc ggccggagct
     9721 cgagtccctg ttttgcgatg cgtttgcttt gatcgcgctc gtgaacacca cggcgcaggc
     9781 ccaacacgat atctcgctcg acgaaacgta tctacgttta ctgcgctcga gtgaacaggt
     9841 ctccgcggcg ttcgacttga cttccctcaa gcaggccctg gagcgcaccc tgcccaccac
     9901 cggcatgggc acggctttcg tatcttgtta cgtggaaggt gacccttcac ggctcgagcc
     9961 cttcgtctgt ctggtcgacg gcaaagcggt cgacccgggc gtggatacct tcccggcctc
    10021 cgcgttcttt ccgcccaatt tgcccggctt cgaaagacgc aaatcgctgc tggcgttccc
    10081 tctggttttc gaggcggagc gcctggggtt ggcggtcttc gagtacgatc cttcggtcaa
    10141 gggctacccc gtcgtcaggg accagatcgc gaccgcgctc cgcaccgtcg ggttgcatca
    10201 agaggtcgtg tacaagacga tgcaacacga gcgcagcgtg caggagcggc tcgcgacggc
    10261 gaagcgaatg aagtcgctga gcctcctcgc gggcggcgtc gcacatgacc tgaacaacgc
    10321 gctggggccg ctcgtcgccc tgccggatgc gatcctgtct gacattcgca acctcaactt
    10381 gcccgagtcc atggtggcgg aggtgtgcgc cgacgtgctc acgattcagt ccgcgtcctt
    10441 gcgggccgcg cacaccatca aagacctgct cacgttggga cgtcagggca ctaccgcgcg
    10501 tcagccgctc gatctgcgcc gcgtcatcga acagtgcgcc acgaacgatg gctcgcgctt
    10561 cgcctggcag agccaacggc aggtcaagtt catgctggac atgcccgcag agccgttggt
    10621 ggtcaaagcc tccgagccgc atatctcgcg cgcgatatcc aatttgattc gaaacgcggt
    10681 ggaggcgatc gccagcgtgg gccaagtcac ggtcaggggc gagttgcgtg tcatcgacga
    10741 acccacgacg cgttacgaga ccatcgaacc gggcgagtat gtcgtcatta ccatcgaaga
    10801 cacgggcagc ggcattcaga atgtggacct gggccgagta ttcgagccgt atttcagtaa
    10861 aaagccgttg gcggacagca gcggcagtgg cctcgggctc gcgatcgtgc acggcgtcgt
    10921 caaggagcac gatggcttca tcgacgtgac gagtgtgctc ggctcgggta cgtccttcat
    10981 gctgtatttc ccgcgcgccg cgggtgagcc cgccacgccc acaccgcgtc gagagtcgca
    11041 cactcgtgcc gctcgcatcc tcatggtcga cgacgacgcg attcagctcc gtacgggcca
    11101 gcgcgtgctc ggtcgattgg gctacgagat caccagcgtc aagagcagcc aagtcgcgta
    11161 cgaggcggct acccagaatc cagctcggcc gttcgatctc ctgattttgg atatgctgct
    11221 cagcgaaggg gaagacgggc tcgagctgtt cgagcggatc cagcgcgtgc ttccgcacca
    11281 acgagcgatc gtggtgagtg gtcacgctcc gaacgagcgc gccgaacaag ccatcaacaa
    11341 agggctgttg tggctgagta agccgtacac ggcagacgca ttggcggatg tagtggagtc
    11401 cgctttgaat ggtcgaggcg taccgttggt ctcgtcgcta gatcgaactt cctccacgga
    11461 gagctccaac gccagcgcca ctccattgca cgaaccctcg agcccgagtt cgctcaatca
    11521 gagcctaccg cgagcgctcc agttcccccc tcgaccgccg gtgctttgag tccacgcgga
    11581 tcgcgccgcc gagctccctt ttatcgcgac tccaccccga tcattttcga gcttcgaggc
    11641 ggggttattg gccggcgctc gcggctcccg cggtgataat ctcggtcggg gcctcgagta
    11701 cagcaccgct gcgaccctgg gttttcacct ttccagagcc ggtgtccagt acctccgcga
    11761 ccacctcgat ggccaccttg acgggacgag tgctgcgcaa accaacatcg aagccgacca
    11821 gccccggaat cgtcgagggc agccagcgtt tgaccaatag gtcgcgatcg gagtgatttt
    11881 ggaaggcgaa ggcgtgattg acgtaagtca gcgtgggtga atcgccgtcg tcgtcggcag
    11941 ggggcggatt ttgaatcaag ttcatgaccg ttgcgaaatc acgcgccatc tcgtcgagat
    12001 catcgaacgt gaacaccccg ctgcgccgag aggccgcgcc cgtgctggtc gcctcgagcg
    12061 agtagtagta atcgatgccg ctcaggttgg gcaggtattc tccctcttca gcgttcacca
    12121 gctggagacc ggggaagtac cgtccgaact cgttccaggg atcgatcaga acctccaccg
    12181 cgtgtggctc gtcatcgagg ttggtgagca cccagctcag ctgcaggcgt gcgtcatcca
    12241 acgtcaacca cggcgcgttc ggaaaaggtg gggcggcggt gccctgtaac gcgctccgtt
    12301 ggctttcggt aggtgccaag atgggaagcg ggacgccgat ccgcgcctcg tacaacgtca
    12361 actcaccatc ttcgtaaatc ggggccatgc tcggatcgag cgcgacttga acgctcggta
    12421 gcgttcgttg agtcgccttc tccgagcagc cgcagagtat cgatgaggcc agcgctcccg
    12481 cggccagtat tcgtaccgcc tgcgctccga cggcccgggc gtacgcgcgg attcccgttt
    12541 ctaccgccga ggtgttcatc agcgcttgct cggccggaac acgaagggaa aggccgcatt
    12601 ggtaggcttg tcggcagttg gaaactgaag cctgccgaat tgccgcgtga tgcagccctc
    12661 cacgcggggg ttggacagag aggagttgga gacgctgacc tggctcaccg agccgcccgg
    12721 tccgatgctg aagctcacgg ttacgccacc cttgagtgtt ggctcccgcg cggctgcgat
    12781 ctcgtaacaa gcttggaaag ctcccatccg ggacatcacg acgcgctgga tttgtccggg
    12841 agagagccct tgtccgggtt tggccgcgtc cttgctagcc agtttgcgtt cgccgccgcc
    12901 gtcaccttcg ccggtgccga gcccacctcg gccattgccg ccgagcccgc gaccaccagg
    12961 ccctgcagcg cctgtgccgt agccaccgcc acgtccggct ccgaaccccg tatccaaggt
    13021 tcccgcgccg aagggcactc catccggacc gactccgccg ccccccggtc cggagccatg
    13081 cagacccatt ccggagcctc gccccagcac tacgttttgc gagcgcaatc cgcccaaagc
    13141 atccgccacc gagctgatgg tattgagcgt cttcttgacc tcgtcgccca cgtcactcga
    13201 gagcacctcc gcaatcccgc cgagtccggt gttggggtcg cccacctgtt cggtcttggg
    13261 cgccttttcg ttgcgaccga gtttgccctc ggtgtgctgg actttgcgtc cgccgccttg
    13321 cgccttgacg tcctttgctc cggggtcttt ttcaccggtc gctggggtgt ccgccttctc
    13381 gctcggcgct tcgagcgcga gctgctcggg cttcaggttg aactttgccg cgagctccgc
    13441 gtccgtggtc aactcgagcg gcttcgggat tagaggcggc gtcgtaatcg cccagatcag
    13501 gagcagcccg ccgcccaccg ccaaacacga gaacacgaac gagagcagta cggcccagtt
    13561 gatgcggccg cgtttctgca gtgcgggggt ggcctcgctg aactgaaaga agaccgagaa
    13621 gctaccgtac tggagcagcc cccagtcccc cggcacgatc gggatcggcg ctccactccg
    13681 ggcgagtaac gccgggtttt cggtgcgtcc acgcagatgc agttcaccac cggttgcgcc
    13741 gcgcgcgtcg agctcccacc cattgcccac cgcgcggatc ggggagtctg cgactgggct
    13801 gccttccggc ttcgcgatca gcgcgccttc accctcgcca acgcgcagcg attgacccga
    13861 atccagcacc cgcgtggcga gcacggtggt tccccagctc gccgccgtac gcagaacgat
    13921 ggctcgtgcc gctgagcttg cccgactcga agacagttgg ttcatcgaaa cgcagcctcc
    13981 ctacgagtgt cagaaagctc ccccaggaag gagcggtgcc cgagcgcacc cgcgcgaaac
    14041 tcggcgcgca ctcggcgtaa gaaatagatg agctggggag acttgaggcg gccttccacc
    14101 tcgatcgccc cgaactgata ggtcttcacg ccctccgggc cttcggcttc cttcacggtg
    14161 gcggaagagg cgctacttgc ggtgccattg cctgctttgg tttccgaatc cttggggcca
    14221 gaggatctgc tcggaagatc gcgctcggct ccccgagctt ccgccggcgc cgtttcgagt
    14281 gactcggcgc gagtcggcga ggcctcggat gccgacttgc cctctgctcc cgccttaccc
    14341 gttgcccgga cgctcttgcc gtcgcgtggt cttttgaccg gcttggttcc cggcttcgcg
    14401 gccttgcccg tctcttccgc atgcgcttcg ggttggtgcg ccgcgaaaac caccgagagg
    14461 gacaccacgc ccgatacgat cagtcgacga acgcgcattc gagcgacctc ctccggagat
    14521 gacgaccggg accggtcagg atccatggac gcaagagcaa ttggggaaac gggaccccgc
    14581 cccgacaatt aagcaccagc cccagtgttc ggctcagccc tggcttcggc gattggcgcg
    14641 atcggtcgaa gtccgccaca ggatgagcgt agcttgaggg ctcaacgccg ccaatccctt
    14701 tgagtttggg gacgttagcc ctacgcgctc ctacaaccgc ggctgccccg ctcgccatct
    14761 gcttgcacgg gttatggaga tggactaggt tccgcgcccc ttcccatggc ctacgcagtc
    14821 atccgcacgg gcggaaagca ataccgagtc gctgaaggcg aagttgttcg cgtcgcgacg
    14881 ctcgcaggta atcccggcga aaaagtccag ttcaacgacg tcctcctgtt gagtggcgac
    14941 accgtgcagg ttggcaagcc ggccctgtcc ggcgccagcg tcgaagccga aatcgtggaa
    15001 cacggccgcg gcgagaaact cgtcgtgttc aagttccgca aacgcaagcg ctctcggcgc
    15061 aaagcgggtc atcgccagga tttcacggcg gtccgcatta ccggagtaaa ggcctgatcc
    15121 atggctcata aaaaaggcgg cggttctacc cgcaacgggc gcggctcgaa cgcgcagtat
    15181 cgcggcgtca aggtgttcgg tggtgagcac gtgcgcgccg gtggcatcct ggttcgtcag
    15241 gtgggtcaga ccatcaagcc gggtcgcaat gtcggcgtgg gccgcgacta cacgttgttc
    15301 gccctcgtgg acggcaccgt gacctacgag cacgacacca agaccaagaa gcgcgtctcg
    15361 gtttatcccg tcagcgctga gcaagcggtc gcggcgacct gagctaccgc gctctcgttc
    15421 tggatattga cggaacgctc gttggagcgg aaggacgtat tgcgccgcgt gatgcggagg
    15481 cgatacgtga gctgcagcgg cgttccatcc ccgtgacgtt gtgcactggc cggatgtatt
    15541 cgggcaccag tgcggtcgcg gatttgttgt ctttgcgcga gccggtggct tgcgtggagg
    15601 gcagccagat cgtggagcac ccaacggggc ggcacttgtg gtccgccccg ttaccctcga
    15661 gtcacgttgc gcctctgttc gaggcgttga acgagcacgg ccccgccgct ttcgtgttcg
    15721 cacgggatcg tgtgttcttc gacggggatg gcgcgcacta cctgcactac gtgaccgggt
    15781 ggtcgcagca agccgagcgc atcgacgcag tggtggacgt gcagcagtgg gaaggcgacg
    15841 ttacgaccct cgcgttggtc gcgctcggtc agcaggtgca gatcgaggcc gtgcacggtg
    15901 tcatccagcg cgaacaccgg gagcacctgc aatcggcctg ttttccgctg gctcgcgcca
    15961 cgatggatgg cgtttggggc ctgctcgtgc gcagtgcacg cgtggacaag ggcaccgcgc
    16021 tcgagtggct ggccgggcac tacggggtga cgacggacga gctcgtcgcg gttggagatt
    16081 ggctgaatga cgtccctatg ttggagcgtg ccggccgatc tttcgccatg aatcaggccc
    16141 cggaagtcgt gaagtctgcg gctgacgagg tgttggtcgc cgatatctgg acgggcggcg
    16201 gcatcgccga ggcggccgaa cgcgcgggct tgctgtgagg ccgtagcgat acggcgcata
    16261 gctggagacg ctgacctgat gtctgcggtc gcaccgaaag tcgcagttct tccgcccgaa
    16321 ttggcgagtc agatcgccgc gggtgaagtc gcggagcggc ctgcgagcgt cgtcaaagag
    16381 ctagtcgaga actccttgga cgccgaggcc acgcggctcg acgtggagat cgagggcgga
    16441 ggcattctgc gcatctgcgt gacggacgac ggcttcggca tgagtgaagc cgacgcgcgg
    16501 ctggcgctgg agcgccacgc caccagcaag ctgcgctcac tcgatgacct catgaacgtg
    16561 cgctcgtatg ggtttcgagg cgaagcgcta cccagcatcg cgtccgtcag tcgcttccga
    16621 ctgtgtacga gggaacggga cgccgagctg gggatcgagc tgttcgtcga ggcaggcgcg
    16681 acccccgtgg cgcggcaaat cggagctcgg gcgggcacgc gcatcgagat tgcagacttg
    16741 ttcttcaatg tgcctgcgcg tcgcaagttc ctgcgctcca gcgcgaccga gtcgggccat
    16801 gtcgcagatg tgatcgagaa cgcggccttg gctcgcccgg acgtgacctt caccttgtcg
    16861 cgtgacggtc gccgcgttcg tgagctgctg cgcgtgacca cgcgcgccga gcgtgtggcc
    16921 caggtcctgg acacgcgaga cttggcgcgt tgcgaagggg agcgcggtcc actcgcagtc
    16981 gaggcctatc tctcgcggcc agaggcagct cgcgcgggcg gggcagggct gcacttgttc
    17041 gtgaacgggc gcaatgttcg cgatcgcgcg ctgctcgcga ccatcgcgca tgcctacggg
    17101 agcgtgctcg agaaggggcg ttacccgcgc ggtgttgtct acctagacat tgcgccggaa
    17161 ttggtcgacg tgaacgtgca tccgcagaag tccgaggtgc gtttcgccga tccgcgcgcg
    17221 gtgagcgacg tggtctatgc gctcgtctcg cgcaacctcg cgagcgcctt ttctctccct
    17281 ccgcaggcga cccaccaccg gtccctctcc gagcgaccct tgccgggcgc aagtcctgat
    17341 cgcgaagctc taaggagctc tactccgcag agtcctagtc ctgagccgga gcggaacacg
    17401 gagcctggct ggggggcggc acgctcgagc ccgcccgtct tcccacttcg tacgagcgaa
    17461 cctgcgagcg ccgcccggac ggtcttcact gtctcggagc ccctgcaccc cctgcacgat
    17521 gccgcggcgc ccctgcgcga tgccgcggag cccctgcgcg atgccgcggc gatgcagctg
    17581 aagaccgcgg agctcgcgcc tctcgaggcg agttcaccgg atccgagtgc gccgcaagtc
    17641 gcatcgatct cggttggcag cactccgtct tcccgacccg catcaacttc cgtcgcgctt
    17701 ccgccggcgc gtcccgagcc gcccgaagcg gaatccaagt ggcagaactt gaggttcgtc
    17761 gcgcagctcc gaaatactta cttgctctgc gaagggtccg cgggaatcta cctgctggat
    17821 cagcatgcgg ccgccgagcg cgtcacattc tcgcgtttgc gcaggcagta tgcggctcgc
    17881 gaagtcggcg cgcagacact gctcttcccc gtactcgtag aattgacgcc cccggaagcg
    17941 gaggtggtgg aggctcgcgc gtcggagctc gcggaggtgg ggctggacgt gcgcatgcgc
    18001 ggctcgagca gcgcgagcct gcactccgtg cccaagctcc tggcgcgcgc ccaccctgag
    18061 cggctgctac gtgatctcct gctcgaagtc acccgcgccg gcgaacgagc ctttagcgat
    18121 gccgtggatc tcgcgctcgc aaccctggct tgccacggca gcgcacgtgc gggagacgag
    18181 ctcggtccgg ccgaggttcg cgcgctcttg ctcggcttgg atgaggccga cttcgcggga
    18241 cactgcccgc acggacgtcc aatcgtcacc ttcacggcgt gggcggagct ggagcgcaaa
    18301 gtcggtcgtc gatgaactcc cgtgcaacac ccgatctccc cgaactgctc gtcatagtcg
    18361 ggccaactgc gtccgggaaa accgatctag ccattcaact cgctgaacag ttgaatggag
    18421 aaatcatcag cgcagacagc gtgcaggtgt atcggcaatt cgacatcggc acgggtaagc
    18481 cgagccacga acaactggcc cgagttcccc accacatgat cggtgtcgtg gaaccggccg
    18541 agcacctcga cgccgcgagt ttcgccgagc aggcgctgtc caccatttca gagatccggt
    18601 cgcgcgcgcg aactccgatt gtttgcggag gtacgttctt gtgggtcaag agcttgatct
    18661 acggcttggc agaggggccc aaagccaacc cggagattcg agctcgccat cgtgagctcg
    18721 tggaacgctc cggtcgggcg gcgctctatg ccgagctact cgagaaagat ccggcgtcgg
    18781 cgcagcggct ctcccccaat gacttcgtgc gggtgagtcg agcgctggag gttctggagc
    18841 tcacggcgat ccccatgagc cgatttcatg cccaacacgg cttcgctcag gcgcgctttc
    18901 gagctcgcct gctaggcgtg cagcggacgc gcgaagagct cgacgaacgc atcttggcgc
    18961 gaactcgggc catgctcgag gctggttgga tcgacgaggt ccgccgcctg gtcgaactcg
    19021 gattctcgga atgtcgagcg atgggctcag tcggctacaa gcaagtgttc gcggcgctcg
    19081 cccaaaccgg ccacccagat cccgatgaat tggcaatctc gatctaccgt gccactcgag
    19141 tcttcgcgcg gcgacagcgt acttggttgc gcgatcaaaa ggtcgaatgg ctagctagcg
    19201 tgtccgaatt cgatagatcc atctcgacag ttccgtaatg taagaactga tcgatcgata
    19261 tccgatcaac ggattggatg ttcagactgg gcgccgcgcc gactccgctc cagctgagga
    19321 ggagtcgaat cagttaccaa atccagcaag ccttacgcgc ggtagagcca gcgctgacct
    19381 caggcgctac catttttctt gggttcgatc gcgaccagat gatgaaatgt gcggactcga
    19441 cgctgg
//
LOCUS       DASTKU010000434         9144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1866782, whole genome shotgun sequence.
ACCESSION   DASTKU010000434 DASTKU010000000
VERSION     DASTKU010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9144
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1866782"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..455
                     /locus_tag="VFQ61_14840"
     CDS             114..455
                     /locus_tag="VFQ61_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955788.1"
                     /translation="MSSLPARPRMRLLEKDAPQTPSDMSTGSTQSPQMAEHRAEPAKQ
                     GSEDSEGPTPRPRRAGARRTRKPQELPPIRGNLGEPVGVLNWERDFLLAHASAVLQDF
                     MSLTEDDEEAG"
     gene            complement(1006..1179)
                     /locus_tag="VFQ61_14845"
     CDS             complement(1006..1179)
                     /locus_tag="VFQ61_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955789.1"
                     /translation="MSGVLGHAGSGTDAVGLALELIDELEHALCEAAIRVVTDGLLNG
                     YHANATPPQGLLV"
     gene            complement(1606..2067)
                     /locus_tag="VFQ61_14850"
     CDS             complement(1606..2067)
                     /locus_tag="VFQ61_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955790.1"
                     /translation="MQRLDLGQAELDLSDLGFQRPKPISADTSELRICLDAFVPALQP
                     FGEHVALALEDATLLGGLLAHDALVLHELAQDLAASLVGEDVLGDVAEHGVVERVDGD
                     PVTLASLFARATEFAAVVVTICRLAVGVEFPGALELASSTALATSQDSGEQ"
     gene            complement(2326..2402)
                     /locus_tag="VFQ61_14855"
     tRNA            complement(2326..2402)
                     /locus_tag="VFQ61_14855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2366..2368),aa:Arg,seq:acg)
     gene            complement(2537..3463)
                     /locus_tag="VFQ61_14860"
     CDS             complement(2537..3463)
                     /locus_tag="VFQ61_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9955791.1"
                     /translation="MQALDPALIPALYDVMIVARTGSVALAARRLNKTPSAVSQQIRR
                     IADVLGVPLFERHGRGLRLTAAAEQVLPAATRLFDEAEAVFRLFGELSGAGVTTLRIA
                     ASDYLAKPLLVPVLRDLAHEQLPLHFEISTVHSEEALTRLERGDVEMAIATFQALRPG
                     VSAIPLFEQAFYWIGPERLGRRRSLVERLTKEPLLRLGPGSFGRKLLDDVLERRGIRP
                     VSTIDVPSVSLLLAYATGGVGIGLVPALPLETRDIRGAVLELAEVPRCPVQLVTRAGP
                     SQVKVVQRFVERLLTEASRQRARLETNDAARR"
     gene            3571..4983
                     /gene="leuC"
                     /locus_tag="VFQ61_14865"
     CDS             3571..4983
                     /gene="leuC"
                     /locus_tag="VFQ61_14865"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET9955792.1"
                     /translation="MGKSLFEKVWDQHTVRTLPNGQAQVFVGLHLIHEVTSPQAFGML
                     RDLGLRVVHPERTFATVDHIVPTDTRLRPFRDDLAEGMIVALEKACREFGIRFFDLSS
                     GHQGIVHVIGPELGLTQPGMTIACGDSHTSTHGAFGAIAFGIGTTQVRDVLATQTLSL
                     APLKVRRIVVRGKLAPGVYAKDLVLHIIRVLGVNGGTGFAYEYAGEAVEALSMEERMT
                     LCNMSIEGGARVGYVNPDQKTFDYLKGREYAPKGENFDRAVEYWRSLASDPDARFDDV
                     VEIDAKDIAPTVTWGINPGQAISIKENVPRAEEGKNDAERASIREALDYMQLEGGAPI
                     EGTKVNVAFIGSCTNGRISDFREVARLIQGKKVAPGVRALAVPGSMSVAEAAEAEGLP
                     EIFRSAGFEWREPGCSMCLAMNPDKLAGRELCASSSNRNFKGRQGSPTGRTVLMSPVM
                     VAAAALRGAVVDAREVFGVS"
     gene            5003..5611
                     /gene="leuD"
                     /locus_tag="VFQ61_14870"
     CDS             5003..5611
                     /gene="leuD"
                     /locus_tag="VFQ61_14870"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET9955793.1"
                     /translation="MALEKIVKVTGRAVYVPGDDIDTDRIIPARFMKCVTFDGLGAHV
                     FHDVRFSEDGSMKDHALNDSRFAGAKILLSNSNFGCGSSREHAPQALAKSGFRAVVAE
                     SFAEIFFGNSTTLGMPCVSVSHDAIVQLSGAVEGDPSLEVGIDLESRTVSAGSLRFPL
                     TIKETARDALMRGAWDPIGQLLDGAEQVRALTARLPYLQFQS"
     gene            5627..6097
                     /gene="tadA"
                     /locus_tag="VFQ61_14875"
     CDS             5627..6097
                     /gene="tadA"
                     /locus_tag="VFQ61_14875"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HET9955794.1"
                     /translation="MRELDHEWMRLALDEADRALEHDDVPVGCVVVSRDGVLLARDHN
                     RREQHQDPTAHAELLGLRAAARANGHWRLEGATVYVTLEPCCMCAGALVHARVARLVY
                     GATDKKAGAVESLFTITTDPRLNHRLQVSSGVLGEEGGQRLTAFFARLRAQGQK"
     gene            complement(6108..8450)
                     /locus_tag="VFQ61_14880"
     CDS             complement(6108..8450)
                     /locus_tag="VFQ61_14880"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HET9955795.1"
                     /translation="MSLWRGLAERWLALASPIRRLVVGGVATAAVAALLLWVVIPWWI
                     ASAVEERAHARGLDVKVGGASVGLGRVRLEHVRARDPLLPGSSVKVESVAISRGWSTT
                     EVHLAGVEVDLSGSTEQLIERWRALFPAKPKPGSEPSSGSARSVEARAISGLWRDPQS
                     GKQVQLWNGLLQQAPDGGVSIGWDRARAVLPEVQLDVKNVQAELSRASVGAQSGGNAG
                     SAEGLSRRFERVQVGSITAELVIRADEAEPVVTPSKVSVGTPGATEARMETWPAKLRA
                     LQSAIRSFSSERFTASVGALILELHRGDESLRIGPSGMRARRGREDAYLDVTPDTNGS
                     SSGTPFAMHAHLPLREGQVSAELSGGPVPLSTLGIREGDMGLVSVREARIEAQAKVAY
                     DPANPIVEISSRGRLENARLRRPALAQQELSDIRLGWRVAGRMSASFDELTLSEGEFS
                     VGDVRVDVAGAIQRDPAPLRLDLKAEMPLTACSALLDALPRGMAPLLTNVRMQGTLAA
                     RGLLHFDIQKPLATNVRLDVKNQCRIAEVPPTISPRRFRNPWTREVKGADGLPMTIES
                     GPGSPDWTPYEDISPYLETAIIVCEDAGFFSHHGLDYRAIENSIRMNLEAGKFLRGAS
                     TVSMQLAKNLYLGREKTISRKFQEAILTQLLEQELSKHELLELYLNVIEFGPGIYGIR
                     QASRHYFNEEPRDLSLGQALYLASILPSPDTQHFAPDGRVTERWSGYLRRLMQIARKI
                     KRISDDELRAAEGEQVAFRKPNENPPRAPDYDSEDVPPEL"
     gene            complement(8579..8695)
                     /gene="rrf"
                     /locus_tag="VFQ61_14885"
     rRNA            complement(8579..8695)
                     /gene="rrf"
                     /locus_tag="VFQ61_14885"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(8882..>9144)
                     /locus_tag="VFQ61_14890"
     rRNA            complement(8882..>9144)
                     /locus_tag="VFQ61_14890"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1811 a         2957 c         2764 g         1612 t
ORIGIN      
        1 gggacgccgg aaggcttacc caaaatcaca aaactcgatc ccgctgagaa taggctggat
       61 cgtgagccct ccctcagatc ccaaaactct cacgataatc acggaaagct ccaatgagct
      121 ccctccccgc ccgtcctcgc atgcggctgc tggagaagga tgcgccacag actccttccg
      181 acatgtccac gggctcgacc caaagccccc agatggccga gcaccgcgcc gagcccgcca
      241 agcaagggtc agaggactcg gagggtccaa cgcctcgccc aaggcgcgca ggcgcccgcc
      301 gaacccgcaa gccacaggag ctccccccga ttcgaggcaa cctcggagag cccgtcgggg
      361 tgctcaactg ggagcgggac ttcttgctcg cccacgcctc cgcggtcctg caggacttca
      421 tgagcctgac tgaggacgac gaggaagccg ggtgaggtgc ggcttaaggc cgcgttcctg
      481 accctttggc caggagccgg cgctcgtgaa acgcatccgg cagctcattt cacgcctcga
      541 tgaacaaatc gcccaatcgt ttcgtcgtgt aaactggagc aaattctgca tgaacgcggt
      601 cagtcgagtg ggtggggacc cgggggcttt cgcaaacgcg ctagcgtcca ccggtctggt
      661 cagcgcccgc acaaacgcct tgtctagccc accgggattc ccatgaagaa gaacgcagcc
      721 gatggcaagc ccatcaaagc agtcggatac gcgcgggtga gtacccctga gcaagcggag
      781 agggacttgt ctctgcctgc tcaggcttca gcgattcgac gctatgcaag cgaccaaggc
      841 tacacgctcg tcaacgagta tgtggagccc ggcatctcag cgcgcgacga cacgcgcccg
      901 gcgtttcggc agatgctcga agaggtgctg cgcccgaact cggggatcga agcgatcatc
      961 gtgacgcaca cctcgcgctt catgcggaac gcgaccaaag cgcgactaca caaggagtcc
     1021 ttgcggcggc gtggcattcg cgtggtagcc attcagcagg ccgtcagtga cgacccgaat
     1081 ggccgcttcg cagagggcgt gttcgagctc atcgatcaac tcgagagcga gaccaacggc
     1141 atccgtaccc gagccggcat ggccgagaac gcccgacaag gttacttcaa cggctcacgt
     1201 gcaccgttcg gatttcgtgt cgagaaaatc tccacgccgg ccgggatgaa gaacaaactc
     1261 gtgcccgacg gtgtcgaggc tgagctcgtg cgcgagctgt ttcgtcagta cattgcgggg
     1321 cgtggcgcca aggctgccgc tcgctacatg aaccagcgcg ggttgctgta ccgcaagcaa
     1381 cagtggaatc gcgatctcgt cctgaaggtc atcagtgagc ccgcatgtat cgggacctac
     1441 tactggggac agctggactc gcgcggcaag tgcgtgcgcc ctgagagcga gtggattgcc
     1501 atcccggtgg agcgcattgt cgacgaggag ctctatcaaa ccgtgcagga gctgcggtct
     1561 caacgggacc ccgttcggaa caccggcagg atagcgacaa gtcctctact gctcaccgct
     1621 gtcctgcgat gtggcaagtg cggtggagga tgccaactcg agagctccgg gaaactcgac
     1681 cccaacggcg aggcgacata tcgttactac aactgccgca aattctgtcg cacgggcaaa
     1741 gaggcttgcg agggttaccg gatcgccgtc gacacgctcg acaacgccgt gctccgctac
     1801 atctccgaga acgtcttcac cgacgagcga tgccgcgaga tcttgcgcga gttcgtggag
     1861 gacgagggca tcatgcgcca gaagaccacc gagcagcgtc gcgtcctcga gcgcgagcgc
     1921 gacatgctct ccaaacggct ggagcgctgg tacgaacgca tcgagacaga tccggagttc
     1981 ggaagtgtcg gcgctgatcg gcttcgggcg ctgaaagccg agatcgctga ggtcgagctc
     2041 agcctggcca agatcaagcc gctgcactcg gtcccgccgt acctttacaa gcccgagacc
     2101 atcgctcggt tccgagcgcg cctgcgcgag acattcctat cgggggacca agcgacggcg
     2161 cgcgtctact tgaacaacct gatcgagacg atcgtggtcg gcgaggacga gatcatcatc
     2221 gaagcgcgag ccaacgccgc acttgccatg atggctggag ccaacgcaat ccactctgac
     2281 gggaacggaa ccaccggggc tccagttctt actgacgtca tagattggcg cgcccgggaa
     2341 gattcgaact tccgacaccc ggttccgtag accggtgctc tatccagctg agctacgggc
     2401 gcgtttctcg ttggcgcgaa cgcctgcgag ggcccgcgta atcccccatt gtgaggcccg
     2461 tgtcaagctg ctttcgcgag gacgcaaaaa ccagcctatc gacctagagc gttgggagcc
     2521 taagaatgcg gtctccttag cggcgagccg cgtcattggt ctccaagcgg gcccgctgcc
     2581 ggctcgcctc cgtcaacaga cgctccacga agcgttggac gacctttacc tggctcgggc
     2641 ccgcccgggt cacgagctgt accgggcaac gcgggacctc ggccaactcc agaaccgcgc
     2701 cgcgaatgtc gcgcgtctcg agcggcaggg ctggcaccag gccaatcccg acgccaccgg
     2761 tagcgtacgc cagcaagagc gagacgctag gcacgtcaat cgtggagacc ggacgaatgc
     2821 cccgcctctc caagacatcg tccagcagct tgcgtccaaa gcttcccgga cccagccgta
     2881 agagcggctc tttggtcagg cgctccacta gacttcgcct ccgccccagc cgctccggcc
     2941 caatccaata gaatgcctgc tcgaagagcg gaatggccga tacgccaggg cgcaacgcct
     3001 ggaacgtcgc gatcgccatc tcgacgtccc cacgctctaa gcgggtgagg gcctcctcgg
     3061 agtgcacggt cgagatctcg aaatgcaacg gcagctgttc gtgagccaga tcccgaagca
     3121 cgggcacgag cagaggcttg gccaagtagt cgctggccgc gatgcgcaat gtcgttacac
     3181 ctgcgcccga tagctccccg aacaagcgaa agaccgcctc tgcctcgtcg aacagtcgcg
     3241 tagcagctgg caaaacctgc tccgccgctg cggtgaggcg caagcctcgc ccatggcgct
     3301 cgaacagcgg cacgcccagc acgtccgcga tacgtcggat ctgctggcta accgccgagg
     3361 gggtcttgtt taggcggcgc gccgcaagcg ccaccgagcc cgtccgagcc acgatcatga
     3421 cgtcgtaaag agcgggaatc agagcgggat ccagtgcctg catgacggcc gagactttag
     3481 aaaagcttac ttgaatatca aggaagtttc attggactta actctaaccc ccgacaatac
     3541 tgcccgaact caccgagcag gaggtccgcc gtgggcaaga gtctattcga gaaagtctgg
     3601 gatcaacaca cggtcagaac gttgccgaac gggcaagcac aagtgttcgt ggggctacat
     3661 ctgattcacg aagtaacgag cccacaagcc ttcggcatgc tccgcgatct cggtctgcga
     3721 gtggtgcatc cggagcgtac gttcgctacc gtcgatcaca tcgtgcccac ggatacccgg
     3781 ctgcgtccgt tccgggatga tttggctgaa ggcatgattg tcgcgctcga gaaggcctgc
     3841 cgcgagtttg gcattcgctt cttcgatttg tcctcggggc atcagggcat cgtgcacgtg
     3901 attggcccgg agctgggttt gactcaaccg gggatgacca tcgcgtgtgg cgactcccac
     3961 acgtccaccc acggtgcgtt cggcgcgatc gcgttcggca tcggcaccac tcaggtgcgt
     4021 gacgtgctcg ccactcagac cttgagtttg gcgccgctga aagtgcgccg catcgtcgtg
     4081 cgcggcaagc tcgctcccgg cgtgtacgcc aaggacttgg tgctgcacat cattcgcgtg
     4141 ctgggtgtga acggcggcac cggcttcgcg tacgagtacg cgggggaggc cgtcgaggcg
     4201 ctgagcatgg aagagcgcat gaccctctgc aacatgtcga tcgagggcgg cgcccgcgtg
     4261 ggctacgtga acccggacca gaaaaccttc gactacctca aaggtcgcga gtacgccccg
     4321 aagggtgaga acttcgatcg cgcggtggag tattggcgct cactggcgtc cgacccggac
     4381 gcgcgcttcg acgacgtcgt cgaaatcgat gccaaggaca tcgcacccac cgtcacttgg
     4441 ggcatcaacc ctggccaggc catcagcatc aaagagaacg ttccgcgagc ggaagagggc
     4501 aagaacgacg cagagcgcgc gtcgattcgc gaggccttgg actacatgca gctcgaaggc
     4561 ggcgcgccga tcgaaggcac caaagtcaat gtggcgttca tcggcagctg caccaacggc
     4621 cgcatctccg attttcgcga ggtcgcgcgc ctgatccagg gcaagaaggt ggcccccggg
     4681 gtgcgagcgt tggcggtacc cggctcgatg agcgtggccg aagccgcaga ggcggaaggg
     4741 ttgccggaga tcttccgtag cgccgggttc gagtggcgtg agcccggctg ctcgatgtgc
     4801 ctggccatga accccgacaa gctcgcggga cgcgagctgt gtgcatcttc ttccaaccgg
     4861 aatttcaagg gtcgccaggg cagcccaacg ggccgcaccg tgctgatgag tccggtgatg
     4921 gtggcggccg ccgcgctgcg tggcgcagtg gtcgacgctc gcgaggtgtt cggagtttct
     4981 tgaggagagt cggagcaatc acatggcact cgaaaagatc gtgaaagtta cgggccgggc
     5041 cgtgtacgta cccggcgatg acatcgacac ggatcggatt atcccggcgc gcttcatgaa
     5101 atgcgtaacc ttcgacggcc tcggcgcgca cgtgttccac gacgtgcgct tctccgaaga
     5161 tggctcgatg aaggaccacg ccttgaacga ttcgcgcttc gccggtgcga agatcctact
     5221 ctctaacagc aacttcggct gtggctcttc ccgtgaacac gcgcctcaag ccctcgccaa
     5281 gagtgggttc cgcgcagtag tggccgagag ttttgcagaa atcttcttcg gaaactcgac
     5341 cacactgggt atgccatgcg tgagcgtcag ccacgacgcc atcgttcagc tgagcggggc
     5401 ggtagaaggc gatcccagct tagaagtggg catcgacctg gaatcgcgca ccgtctcggc
     5461 gggctcgctg cgctttccgc tcacgatcaa ggagaccgcg cgtgacgcgc tgatgcgtgg
     5521 agcgtgggat cccattggcc aattgctcga cggcgccgag caagtccgtg cgttgacggc
     5581 tcgcttgcca tacctccagt tccaaagctg acccgcacgg cgccggatgc gggagctcga
     5641 tcacgagtgg atgcgcctgg cgctggacga ggctgatcga gcgctcgaac acgacgacgt
     5701 acccgtcggc tgcgtcgtcg tgtcccgaga tggggtcttg ctcgcccgcg atcacaaccg
     5761 ccgggagcaa caccaagacc caaccgccca tgccgaactg ctcggcttac gcgccgcagc
     5821 ccgcgccaac ggccactggc ggctcgaagg cgccaccgta tacgtcaccc tcgaaccttg
     5881 ttgcatgtgc gcgggcgcgc tggtgcacgc tcgcgtcgcc cgcctggtct acggcgcgac
     5941 ggacaagaag gcgggcgcag tagagtcttt gttcacgatc accaccgatc caaggctcaa
     6001 ccaccgtttg caggtatcgt ccggggtgtt gggcgaggag ggcgggcagc gcctaacggc
     6061 gttcttcgcg agactccgcg cccaaggcca gaagtaagca tccggaacta gagttccggg
     6121 ggcacgtctt cggaatcgta atcgggagct ctcggcgggt tctcgttcgg ctttcgaaac
     6181 gctacctgct cgccctctgc ggcgcgcaac tcatcatcgc tgatgcgctt gatcttgcgg
     6241 gcaatctgca tcaggcgcct caaatagccg ctccaacgct cggtaacgcg accgtccggc
     6301 gcgaagtgct gcgtatctgg gctcggtaga atggaggcca agtagagcgc ctgccccagc
     6361 gatagatcgc ggggctcctc gttgaagtag tgtcgcgagg cctgccgaat cccatagatc
     6421 ccgggcccga actcgatcac gttgagatac agctcgagca gctcgtgctt ggaaagctcc
     6481 tgctccaaga gctgcgtcag gattgcttcc tggaacttgc gtgagattgt cttctcgcga
     6541 ccgagataca ggttctttgc cagctgcatg ctgacggtac tcgcgccgcg caggaatttc
     6601 cctgcctcga ggttcattcg aatagagttc tcgatcgcgc gatagtcgag cccgtgatga
     6661 ctgaagaagc ctgcgtcctc acagacgatg atagcggtct cgagataagg cgaaatgtcc
     6721 tcgtacggag tccagtctgg cgatccagga ccgctctcga ttgtcatcgg gaggccgtcg
     6781 gcgcctttga cctctcgcgt ccacggattg cggaaacgac ggggcgagat cgtaggcgga
     6841 acctccgcga tgcggcattg attctttacg tccaagcgca cgttcgtcgc gagtggcttc
     6901 tgaatatcga aatgcagcag accccgggcc gccagcgtgc cctgcatccg gacgttcgtc
     6961 agcagcggcg ccatgccccg gggcagcgcg tcgagcaaag cgctgcaggc cgtcaaaggc
     7021 atctctgctt tcagatccaa gcgtagcggg gccgggtcac gctgaatggc tcccgccacg
     7081 tccacccgga catcccctac ggagaactca ccttcggaca acgtgagctc gtcgaaggaa
     7141 gcactcatgc gacccgccac gcgccagccc aagcgaatat cgctcaactc ctgttgcgcg
     7201 agcgcaggac gtcgaaggcg tgcattctcc aagcgaccgc ggctactgat ctcgacgatg
     7261 ggattggccg gatcgtacgc gaccttggcc tgagcctcga tacgcgcctc gcgaacggag
     7321 accagtccca tatccccctc gcgaatgcca agcgtagaca acggaaccgg gccgccgctc
     7381 agctcggcag acacctgccc ctcacgcaga ggcagatgcg cgtgcatcgc aaacggagta
     7441 ccgctggagc tcccattcgt gtccggagtc acgtctaggt aggcgtcctc gcgcccacgt
     7501 cgcgctcgca tcccactcgg accaatgcgc agagactcat ccccgcgatg aagctctaga
     7561 atgagcgctc ccacggatgc ggtgaagcgc tccgagctga acgagcgaat cgccgactgg
     7621 agagctcgca gcttggcggg ccaggtttcc atgcgggcct cagtcgcacc aggcgtaccg
     7681 acgctcacct tcgagggagt tacgaccggt tcggcttcgt cggctcgaat cacgagttca
     7741 gcggtgatgc tgcccacctg gacgcgttcg aagcgtcgac tcagcccttc tgcgctgcct
     7801 gcgttccctc cgctctgcgc accgacagac gcgcgcgaca gctccgcctg gacgttcttc
     7861 acgtcaagct gcacctcggg caacaccgcg cgcgcgcggt cccatccaat agaaacgccg
     7921 ccatcgggcg cttgctgcaa caagccgttc cagagctgaa cctgcttgcc cgattgcgga
     7981 tcgcgccaga gcccgctaat cgcgcgagcc tcgacacttc gagcgcttcc gctggagggc
     8041 tcggatcctg gcttcggctt agcgggaaac aatgctcgcc agcgctcgat caactgctcc
     8101 gttgagcccg agagatcgac ctcgacccca gcgaggtgaa cctccgtcgt actccacccc
     8161 cgtgaaatcg cgacggactc caccttcacg ctagagccgg gcagaagcgg atcccgggct
     8221 cgcacgtgct ctaaccgtac tctgccgagc cctacggacg cgccgcccac ctttacatcg
     8281 aggccgcgag cgtgagcccg ctcttcaact gcactcgcga tccaccatgg gattaccacc
     8341 cacagcagga gcgccgccac tgcggccgtt gccacgcccc caacaacgag tcgtcgaatc
     8401 ggactcgcca gcgccaacca gcgctccgcc aatccgcgcc ataagctcat caagtgcgtg
     8461 gagcttaacc cgagactgcc acccccacac aagaccgatt cttagaccgt ccagccgacc
     8521 tgctcattag acaaagaaaa ggccctgagt gttacctcag ggccccttca ggcaattagc
     8581 ccggcagcgt cctactctcc cacacggttg cccgtgcagt accatcggct ccgaagagct
     8641 tgacttccga gttcgagatg ggatcgggta tggcctcttc gatagagcca ccggaaatct
     8701 ttgggcgtcc gaatcgcgag gagattcagt gtcttcgaca gactctatgt ctcaactcgc
     8761 tagctcgcta tgcattggtg aatgctacgc gctgtgtcgg ttcgtgcaga gtgagtcggt
     8821 agttaccaac tcgaactcac gcccggactc cgaccaccaa caatgatgtt tgccttaggt
     8881 ttaggtcaag ccgcacggcc tattagtacc ggttagctcc acgtcttgcg acgcttccac
     8941 acccggccta tcaacctcgt cgtctacgag gggcctttag tgccttgcgg cagggatacc
     9001 taatcttgag gccagcttcc cgcttatatg ctttcagcgg ttatctgttc cgcacatggc
     9061 tacccggcta tgccagtggc ctgacaaccg gatcaccaga ggtacgtcca cccaggtcct
     9121 ctcgtactat gggtagctcc tctc
//
LOCUS       DASTKU010000435         9608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1421468, whole genome shotgun sequence.
ACCESSION   DASTKU010000435 DASTKU010000000
VERSION     DASTKU010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9608
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421468"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1171)
                     /locus_tag="VFQ61_14895"
     CDS             complement(143..1171)
                     /locus_tag="VFQ61_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955796.1"
                     /translation="MVSLGFLPLFTLGVARAQSAPAIVPFSLQYTAPQGCPTREDFEG
                     AIVRRAPRAHSTIVGAGVTRFNAELVELGPGRVRGSLRVAQPDGAETRRAVDAQSCAQ
                     AIDYMALIAALILDPAAQALPTSPSQPLLPAGEGPSETEARVSPEPTPTGAERRLEKN
                     RTEPAASHSKVATTRVRAGAWLGGTRESGVAPSAPLGMVLGVEASLDRASSWSPSLQA
                     SFLYTARAEEEMQSSGEASFRLIAARLALCPWRSPGPLRFRACAEFDAGALVVDAPQA
                     QNMPWLAAGPSLQAEVDTGRVLALRLNLGAHGLLHHDRFLSGGDQLVYDVPAVSWHLD
                     LGLVVRTP"
     gene            1686..4082
                     /locus_tag="VFQ61_14900"
     CDS             1686..4082
                     /locus_tag="VFQ61_14900"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9955797.1"
                     /translation="MRFVYDTGLRRKLFQNVRLLGTWDKRGRHCDEWSEIPMREGLGP
                     DGSVVFEAEVPLAPSEVGKEFKWSVRLDGPLGLDREGITSEQLGLGHDALHRTFTLGD
                     KGQGEQRYYLTHVRRLGARKVSLPGNKGYGLRFAVWAPNAEAVEVVFAKPSVGYIADD
                     GTGIDETRPVIKLADAEDGIWEAGPAQNPALRRFEDFVNAPYMYRIRMKSGRVAYRTD
                     LFSRAQVGSGEVNPNGAPYDGDPKELDGVVSCSLVVDTELVDMGDGTQLQEDEFWRDE
                     FNHATPFPLRMEDMVIYELHVGSLGFGKERPGNFEDAIELLDYLVDLGVNAVELLPIA
                     EANGTRTWGYGNSHHFAIESSAGGRDAFRRFVREAHRRGIAVFVDVVYNHYVPNGERA
                     QWHYDSEAEEENIYYWYEGKVSDYPSPDGGYLDNLSTGYAPRFHEETVRKFFISSAVA
                     LVMDFHVDGFRVDQTTSIHAYNVVHADGRPAGNANQAGAKFLRELSRTLRTIRPSTLL
                     TCEDHSDWVLVTEHPDGAGLGFDATWYSNFYHQLIGDTGRGADCANLLHTAGMGGNGP
                     LAMDRFAEALLVSCRKTIVYHESHDEAGNSEHSARTIQVAIAADKGAPPATGDLRRIA
                     EARCRWVAGITLLSAGTPMFLMGEEVGASKPFTFDGFMENREDLLGERKGAGKHLFRF
                     YSELIRLRRRHHTFAAANIEVVSVHNDARLIAFRRWKGPENYLVLATLSDTGFPEGYE
                     LIAEAVPNGNWREIFSSDAEQFGGDGLTNDSEIKAEDHRMTVKIPARGFVVLRHVPGT
                     "
     gene            complement(4093..5055)
                     /locus_tag="VFQ61_14905"
     CDS             complement(4093..5055)
                     /locus_tag="VFQ61_14905"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9955798.1"
                     /translation="MQTQSELWAIVLAGGSGKRLEPLTSALYGAAVPKQFAAIAGQQT
                     LLTQTLARTARLVPESQIVVVATQTHAALARREQNGFPAIQLVLQPSNLDTGIGILVG
                     LTQVLARSPDARVLVLPSDHYVRDESRFLSALSDAAICGATCDIALALVGVRPQAAER
                     EYGWIVPGPALPSPGFDARAGKLTALRRVERFVEKPSTELANDLYHQGALWNTLAFSG
                     SARSCWRLCAECLPEVVKGFASYSETVGTAREDAALNALFSELGSTNFSERVLTRAAP
                     LLGVVECADVGWSDIGTPARVFESLKGTPEALLLEQRLAQQRSL"
     gene            complement(5322..5404)
                     /locus_tag="VFQ61_14910"
     tRNA            complement(5322..5404)
                     /locus_tag="VFQ61_14910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5369..5371),aa:Leu,seq:taa)
     gene            6177..8027
                     /locus_tag="VFQ61_14915"
     CDS             6177..8027
                     /locus_tag="VFQ61_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955799.1"
                     /translation="MFALAMASLLTSTVGCRDFWGIQEPTLASGGTHGGSPSGSQGGR
                     SQGEQSSVENGGASGEAATDRTGGAPSDSGAGGVLQGGDRNGSAAAGASEGGATGGVN
                     SETGPAGPAAQGGAWGGDSGSGGGNELTFRDSAELIAQRRLKFPVANSALPTAIDLSE
                     GFPPVADQGSTPASVAWAAAYALKSYQEGLEEKWDLGVLHHQFSPYWILSQTAVRSSA
                     GEVLGAYPAAVLDVIVNRGCDSLMSSTSSVANAEDLTEASLHRARRFKAASWGTLDVS
                     VDVFKERLASGHAILVSFEALEDFRRLSAENPIFDTSDGPSAGRHTAVIVGYDDRNSV
                     FSLMNSQGKAWGVSGYGKLHYSLLSDRKLALDAYVLIDEVNQEPDLDNLYAITDGYLV
                     RLDRDFGDSVVLRPGSDWRHVSSAAGLTDRLYIVDGQLLHSVSPQDGSYEVLGVGEWQ
                     GQTRMTILGAKLYITQGQDLWEVNPITGTYVWFDGPFWVSPDALVSLNGWLYLVDGET
                     LYLVDPGNGSATALGVEKWPESSQLAALEGKLYAMREGAVLQVDALTGATTPLGVSGL
                     TPSMGFTGLQGRLYVAQPGGVVEIEPRTRALRRLNMDSGWSSSDRLVALP"
     gene            8024..9109
                     /locus_tag="VFQ61_14920"
     CDS             8024..9109
                     /locus_tag="VFQ61_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955800.1"
                     /translation="MSSRLSPAHLRRLSVLLLSGCLLAGTSHARDPALAESLFIEGKK
                     LLRSGEHARACELLAESNRQDPASGTLLALALCFEKAGRVASAWATYSDAAALAAHEH
                     RPDRERSARERVRVLEPLLPRMRVEIPAQVRELPGLTVLRNGDVWPVAARDSAVPMDP
                     GPVTLEVAAPGFQVWRAELQISDQPTTTQVEVPMLVASPPPQPQVAAEASLGADRELP
                     PGQARDTPPEPCTRILSNQAQVPPAPRERSYAAEWALGGIGVAALGTSAYFGIRAGVL
                     NSRSNRPGHCDGNLCSSQGYEERVRAGVAADMSSVLLVGGAGLLVAAGATYWFRSKAF
                     LPRPAPVRLASAALTRGGASFTLQGHF"
     gene            complement(9132..>9608)
                     /locus_tag="VFQ61_14925"
     CDS             complement(9132..>9608)
                     /locus_tag="VFQ61_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955801.1"
                     /translation="GTIAYSTREFTLTNTPVARAWEFASDKEEWLFSNQVSGFTSSNG
                     TLGGAIVGPSAEIFTSGNLGVPIGPASNQKVRIRLKNTSAATQAKFYWLRDDDLTWTT
                     SKSKVFAINANSDWTEYLVDLSSVPGWRGVMHTFNLYPALGVSSGSFSVDYIHIEP"
BASE COUNT         1796 a         2917 c         3149 g         1746 t
ORIGIN      
        1 ccgcccggaa actgcagggc gcaggtgcgg gggaggggga gggggagcga gatgggcgcg
       61 cgccatacgt gatcatcggg cgatgtcgcc ccccgttcgg atcgatccga gccggacgaa
      121 aaaagagcaa gctcagtatg ggctacgggg tgcggacaac cagccccaaa tcgagatgcc
      181 acgaaaccgc cggcacatcg tagacgagtt ggtcgccccc agacaggaac cggtcgtggt
      241 gcagcaggcc gtgtgcaccc aagttcaatc gcaaggcaag gactcgcccc gtgtcgacct
      301 ccgcttgcag cgaaggccca gctgccaacc aaggcatgtt ttgcgcctgg ggagcgtcca
      361 ccacgagggc gccggcatcg aactcggcac aagcccgaaa ccgcagcggg cccggggagc
      421 gccatgggca gagtgcaaga cgggccgcga tcaaacggaa gctcgcttca cccgacgatt
      481 gcatctcttc ttcagcgcga gcggtgtaaa gaaaactcgc ctgcaggcta ggcgaccagc
      541 tcgaagctcg atcgaggctc gcttccacgc ctaaaaccat gccaagcgga gccgaaggag
      601 cgaccccgga ttcccgcgtg ccccccagcc aagcgcccgc ccgaacgcgc gtggtcgcca
      661 ccttggagtg actcgcggcc ggctcggttc gattcttttc gaggcgacgt tcggcacctg
      721 tcggagtggg ctcaggcgaa acacgagcct ccgtttctga tggcccttct cccgcgggta
      781 gtagtggctg ggacggtgac gtgggcaggg cctgggccgc cgggtccaag atcagggcgg
      841 cgatcagcgc catgtaatca atcgcttgcg cgcagctttg ggcgtcaacg gcccggcgcg
      901 tctcggcgcc gtcgggctgc gccactcgca atgagcctcg aactcttccc ggcccgagtt
      961 cgaccagctc agcgttgaag cgcgtgaccc ccgctcccac aatcgtcgag tgggcacgag
     1021 gcgcgcgccg cacgatcgct ccctcgaaat cctcgcgcgt cggacaaccc tggggcgccg
     1081 tgtattgcag actgaaaggc acgatcgcgg gcgccgactg ggcccgggct actcccaggg
     1141 tgaacagggg aagaaagccc agactaacca tcccccagcc gaccaacgct cgaccgctgc
     1201 atcgtttgga gcgaatcata tccggtcgac gccaatcgag aggacgaacc ggcatgggtg
     1261 acgggaaggc aacgcgccgg atctatcgag agccgaacgt taggtcaagg ctgcgttaca
     1321 gccgtgcgtc cggattgagg gcaattgccg tcgggaagac tcgttaggcc acgcacgtcg
     1381 acacgacggc cgtcggcttc ggtacggacg acgcgagcca ccgcacctcg acactccagg
     1441 ccactgttgg atgcagctat agagatgtcg gcatcgctat tgaatagctt cgctgccggc
     1501 gtgcgcgagg ctcgcaattg gtcgaagcgg aacaccaaat caccccagtc gagaaaaacg
     1561 ccccggcagg gttgccttac ccactcgagc gacgggttag aaatgcgggt gatccgagca
     1621 cccacaaagc gtgacttttt cggcactctc cctgcattga gcgtaactcg aaccgaagga
     1681 tatcaatgcg attcgtttac gatactgggt tacgccgcaa gttgttccag aatgtgcgcc
     1741 tgcttggaac ctgggacaag cgaggtcgcc attgcgacga atggtccgaa atacccatgc
     1801 gtgaagggct tggcccggac ggctctgtcg tattcgaagc ggaggtgcct cttgcaccct
     1861 cggaggtggg taaagagttc aagtggagcg tgaggttgga cggtcctcta gggctcgatc
     1921 gtgaaggaat caccagtgag cagctcgggc tcggacacga cgcgctccac cggactttca
     1981 ctttggggga caagggacag ggtgaacagc gttactacct gacgcatgtg cgccgcttgg
     2041 gcgcccgcaa agtgtcactc cctggaaata agggatatgg gctccgcttc gcggtttggg
     2101 ctccgaacgc cgaggcagtc gaggtcgtgt tcgccaagcc ttcggtgggc tacatcgcgg
     2161 atgatggaac tgggatcgac gagactcgac ccgtgatcaa actggcagat gccgaggatg
     2221 ggatctggga ggccgggcct gcacagaacc cggctctacg ccggttcgag gatttcgtaa
     2281 acgcacctta catgtaccgc attcgtatga agagcgggcg tgtcgcgtat cgcacggatc
     2341 tattctcgcg cgcccaggtg ggcagtgggg aagtcaaccc gaacggcgcg ccctacgatg
     2401 gagatccgaa ggagctggac ggggtcgtga gttgttcgct cgtggtggac accgagctcg
     2461 tggacatggg tgatggcact cagctccagg aagatgagtt ttggcgggac gaattcaacc
     2521 atgcgacgcc cttcccgctg cgcatggagg acatggtcat ctatgaactg catgtgggct
     2581 ccctcggctt cggtaaagag cgcccgggca atttcgagga tgccatcgag ctgctcgact
     2641 atctggtcga tctcggggtc aatgccgtcg agttgctccc catcgccgag gcgaatggga
     2701 cccggacctg gggttacggt aactctcacc acttcgcgat cgagtcgagt gcgggcggaa
     2761 gggacgcctt cagacgcttc gtgcgcgagg cccaccgacg cggaatcgcg gtgttcgtcg
     2821 acgtggtcta caaccactac gtgccgaacg gagagcgggc ccagtggcac tacgactctg
     2881 aagcggaaga agagaacatc tactactggt acgaaggcaa agtcagtgat tacccgagcc
     2941 ccgacggcgg gtatctcgac aatctatcga ccgggtacgc gcctcgcttc cacgaagaaa
     3001 cggtgcgcaa gttcttcatc agcagcgctg tggctctggt catggacttc cacgtcgacg
     3061 gcttcagggt cgaccagact acgtcgattc acgcctacaa cgtcgttcat gcggatgggc
     3121 gccccgccgg gaacgcgaat caggctgggg cgaagttctt gcgtgagctg agccgcacct
     3181 tacgcaccat tcgcccatcg acactgctca cttgtgaaga ccactccgat tgggtgctgg
     3241 tgaccgaaca ccccgacgga gcgggcttgg gcttcgatgc gacgtggtac tcgaacttct
     3301 atcatcaact catcggtgat actgggcgcg gcgcagactg cgcgaacctg ctgcacaccg
     3361 cgggcatggg cggtaatggc ccgctcgcca tggatcgctt tgccgaggcg ctgctcgtct
     3421 cgtgccgtaa gaccatcgtt tatcacgagt cgcacgacga agcgggcaac tcggaacact
     3481 ccgcgcgcac gatccaggtg gcgattgcgg cggacaaggg agctccaccg gcaactgggg
     3541 atctgcgtcg cattgccgag gcacgctgcc gatgggttgc gggtatcacg ctgctctcgg
     3601 ccggaacgcc catgttcttg atgggcgagg aggtcggcgc gagcaagccg ttcacgttcg
     3661 acggcttcat ggaaaatcgc gaagacttgc tgggagagcg caagggtgca ggcaagcacc
     3721 tgttccggtt ctatagcgag ctgattcgtt tgcgccgccg acaccatacg ttcgcggccg
     3781 ccaacatcga ggtggtctca gtgcacaacg acgctcgctt gattgcgttt cggcgttgga
     3841 agggcccgga gaactatctc gtgctcgcga ccctcagtga cacgggcttc ccggagggtt
     3901 acgagctgat cgccgaagcg gtcccgaacg ggaattggcg tgagatcttc agcagcgacg
     3961 ccgagcagtt cgggggcgac ggtctgacca acgactccga gatcaaagcc gaagaccacc
     4021 gcatgacggt caaaattcct gcgcgaggct tcgtggtact caggcacgtg ccgggtacgt
     4081 gagcacgtgc gctcataggc tgcgttgctg cgcgaggcgt tgctctagaa gcaacgcctc
     4141 cggcgtgccc ttcagagact cgaagacacg agcgggagtt ccgatatcgg accacccgac
     4201 gtccgcgcac tctacgacgc cgagcaacgg tgccgcgcgc gtgagtacgc gctcactgaa
     4261 gttcgtactg ccaagttcgg aaaacagtgc gttcaacgcc gcgtcctcac gcgccgtgcc
     4321 cactgtctcg gaatagcttg cgaagccttt gacgacttca ggcaaacact ccgcgcatag
     4381 ccgccagcaa gagcgggccg agcccgagaa tgccagcgtg ttccagagtg ccccttgatg
     4441 gtacaggtcg ttcgccagtt cggttgacgg cttctcgacg aagcgctcta cgcggcggag
     4501 cgcggtcagc ttgcctgctc gtgcatcgaa tccagggctg gggagcgcgg ggcccggcac
     4561 aatccagccg tactcacgct ccgcggcctg cgggcgcacc ccgaccaacg ccaatgcaat
     4621 atcgcacgtt gctccgcaga tcgccgcgtc gctcagagcg gacagaaagc gactctcgtc
     4681 gcgcacgtaa tgatcggacg gcaagactag cactcgcgca tcgggagagc gggccagtac
     4741 ctgcgtcagc cccaccagaa tcccaattcc cgtatctaga ttgctcggct gcagcacgag
     4801 ttgtatcgcc ggaaaaccgt tttgctcccg acgcgccaac gccgcgtgtg tctgcgtcgc
     4861 caccactacg atttgcgact cggggaccaa ccgcgccgtg cgtgcgagcg tctgcgtcag
     4921 caacgtctgc tggcccgcga tcgcggcaaa ctgcttggga actgcagccc cgtacagagc
     4981 cgaggtcagc ggctccagtc gcttccccga acctcctgcg aggacgatcg cccacagctc
     5041 ggattgggtt tgcatgggcg cgatcgaagc acggctgctc gacgtcgcct aggcctgctg
     5101 ggcagcggca gcagagtaat tcaatcgctc gggggctggc ggaagccgct cgattcgcga
     5161 tcaccaaaaa cgaacgccat cctcacaggg atggtaacga tcctgaaggc attcatgtcc
     5221 cagcaacgag gacgttctgc agaaagactc cgtcacctcg cggggagccc acccaacttt
     5281 cgggcgcgct atctcgcgcg gagatcccac ctccgtggcg gtagcccctc ccggggtcga
     5341 accgggacgt cctttcggac aagggatttt aaatcccctg cgtctgcctt tccgccaagg
     5401 ggccttgggc gcgatttcca tagcttttgg cgcatcttgc gctcggtgtc cagaggatac
     5461 ttccaaggcc tacaggggag gggctagtgt gggcccgctg gaaccgctgg ctcgctgccc
     5521 gctgcgtcgg tgctggaggc ggcgcgcatc cgggaagccg tagttctctc acccgatgct
     5581 ggcgcagcgc gttccgtgct gcccgagcgc cttgagtgcg gctcccgcgt tgggctagga
     5641 gatgagccgc ccagtgcgcg ccagcccata ttcgacggag gcgaggcgcc gtcggcggcg
     5701 gcgttccgaa ccccgccgcc gaccctgccg gcgagcgcga cgcggtggag tatggggctt
     5761 cgtcaggtga gtggcctcag cgagcgcgca cgcccaaccg ggaggtgttt acgccccgaa
     5821 ggaaaggtca catcgtttca cgggcgttct cggaggatga gagttcgaga gactcgaagt
     5881 taccaatacc aattcttgga gttcggactg ggtcgctcag actgagtcgc tccagatatc
     5941 caacaagagt attggggtcg ctggcgaccg tgaagtagac cgcagcgggg cgctgttctg
     6001 gtctctgtcg gggcggcgcg catgcccgcg cgcaacgccg ggtacgtcgc tcgtaaagca
     6061 cgctgcgctg cgccgcgctg atcgccgttc gcaccggctg gggttgcctt gacgaggagg
     6121 gggcccgcat gtagtacatc cccggcaagc cgatgggttc gagttccagc cgttgcatgt
     6181 tcgccttggc tatggcttcg ttgctgacat caaccgtggg ttgtcgtgat ttctggggaa
     6241 tccaagaacc cacgctcgcc tcgggcggaa cgcacggtgg tagcccctcg gggagtcagg
     6301 gcgggcggag tcagggtgaa cagagcagcg tggagaatgg cggggcttcg ggcgaggcag
     6361 ccaccgatcg cacgggcgga gcaccttcgg acagcggagc cggcggcgtt ctgcaggggg
     6421 gcgaccggaa tgggagcgcc gcggccggcg cgagcgaggg cggcgccaca ggcggggtaa
     6481 actcggaaac ggggccagcg gggccggccg cgcagggcgg agcctggggc ggggattccg
     6541 gttcaggcgg tggcaacgag ttgacgttcc gagattccgc agaattgatc gcgcagcgcc
     6601 ggctgaagtt cccagtagcc aattcggctc tgcccacagc catcgacctt tcggaagggt
     6661 tcccgccggt tgccgatcag gggtcaactc cagccagtgt ggcttgggcg gccgcgtatg
     6721 cgctgaagag ttaccaagaa gggctcgagg agaaatggga cctcggcgtg cttcatcatc
     6781 agttctcacc ctattggatt ctcagccaaa ctgcagtgcg cagcagcgcc ggtgaggtgc
     6841 taggcgctta ccccgcagcg gtactggacg tgatcgtgaa ccgcggttgc gactcgctca
     6901 tgtcctcgac tagcagcgtg gccaacgcgg aggacttgac cgaagcatcc ttgcatcgag
     6961 cgagacggtt caaagctgcg agttggggaa cactggatgt ctccgtggac gtcttcaagg
     7021 aaagattggc gtccgggcac gccatcttgg tctctttcga ggctctagaa gatttccgtc
     7081 gtctgagcgc ggaaaacccg atttttgaca cttcggacgg gccgagcgcc gggcggcaca
     7141 ccgccgtgat cgtgggatac gacgaccgca actcggtatt ctcgttgatg aactcacaag
     7201 gtaaagcttg gggcgtcagc ggctacggca agctccatta ctcgctcttg agcgaccgca
     7261 agctcgcgtt ggacgcgtat gtattgatcg atgaggtcaa tcaagagcct gacctcgata
     7321 acctgtacgc gatcaccgat ggctacctgg ttcgcttgga tagagacttc ggggactcgg
     7381 tcgtgctgcg cccaggctcg gattggcgtc atgtgagttc ggccgcgggc ctgacggata
     7441 ggctgtacat cgtagacgga caacttttgc actccgtatc tccgcaagac ggatcttatg
     7501 aagtcttagg cgtcggcgag tggcagggtc agactcgaat gacgatcctg ggagccaagc
     7561 tgtacatcac gcaggggcaa gatctgtggg aggtcaaccc gatcacaggg acgtacgtat
     7621 ggttcgatgg gccgttttgg gtctcgcccg acgcactcgt ttcattgaac ggctggcttt
     7681 atctcgtgga tggggagacg ttgtatttgg tggatccggg gaacgggtca gccacggcgc
     7741 ttggtgtcga gaagtggccc gagtctagtc agttggcagc gctcgagggc aaactgtacg
     7801 caatgcgtga gggagcagtt ttgcaagtgg acgcgttgac cggggccacc acgccactag
     7861 gcgtatccgg gctaactccc agcatgggat tcactgggct gcaagggcgc ctctacgtgg
     7921 cgcaacctgg cggcgtagtg gagatagagc ctcgcacccg cgctttgcgc cgcctaaata
     7981 tggactcagg ttggtccagc tccgatcgct tggtggcgtt accatgagtt cgcggttatc
     8041 gcccgctcac ctacggcgac tctcagtcct tctgctctcc ggctgtctgc tggcggggac
     8101 cagtcacgcg cgcgaccccg ctttggcaga gtccctgttc attgaaggca agaagctcct
     8161 gcggtcgggc gagcacgcca gggcttgcga gctgttagcg gagagcaatc ggcaagatcc
     8221 cgcttcgggg acgctgctcg cgttggcgct gtgcttcgag aaagccgggc gggtggcgag
     8281 cgcctgggcc acatacagtg acgccgcggc cttggccgca cacgagcacc gcccggatcg
     8341 cgagcggagc gcgcgagaga gggtacgcgt gctcgagccc ttgctcccgc ggatgcgcgt
     8401 cgaaattcct gcacaggttc gtgagctccc ggggctcacg gtactgcgca acggggacgt
     8461 gtggcccgtc gcagcgcggg atagcgcggt gccgatggat ccggggcccg tgactctcga
     8521 ggtggcggcc ccgggttttc aggtctggcg tgcggagctc cagattagcg atcagcccac
     8581 gacgactcag gtcgaggttc cgatgctcgt ggcctcgccg ccaccccagc ctcaagtggc
     8641 ggccgaggcg tcgcttggcg ccgaccgaga gttgcctcct ggccaggctc gcgacacgcc
     8701 gccggagcca tgcactcgaa tcctctcgaa ccaggcgcaa gtgcctccgg cgccgcgcga
     8761 gagatcctac gcggcggagt gggcactcgg tggcatcggg gttgcggcct tgggtacgag
     8821 cgcgtacttc ggcattcgcg cgggggtgct caactcgcgc tcgaaccgcc cgggacattg
     8881 tgacggtaat ctctgctcct cccaaggata cgaagagcgc gttcgggctg gagtcgccgc
     8941 ggacatgtca tctgtactgc tcgtgggtgg cgcgggcctg ctcgtcgccg cgggcgccac
     9001 gtactggttc cggtccaaag cgttcctacc ccgacccgcg ccggtgcgtc ttgcttccgc
     9061 cgctctgacg cgcggcgggg cgtccttcac ccttcaaggt catttctgag tctggcacgc
     9121 cgcagcgtgc ttcacggttc gatatggata tagtccacgc taaatgaacc gctgctcacg
     9181 cccaaggccg ggtagaggtt gaaggtgtgc atcacgcccc gccaacccgg cacgctggac
     9241 aaatcgacca aatactctgt ccagtccgaa ttcgcgttga tagcaaaaac cttcgacttg
     9301 ctggtggtcc aggtcaggtc gtcgtctcgc agccaataga acttggcttg ggtcgcggcg
     9361 ctcgtgttct tgaggcgaat gcggaccttc tgattacttg cgggaccgat cggaacgccc
     9421 aagttgcccg aggtgaaaat ttccgcgcta ggcccgacga tcgccccacc cagggtgcca
     9481 ttcgaagacg tgaagccgct cacctggttc gaaaatagcc attcttcctt atcggacgcg
     9541 aactcccagg cgcgagcgac gggcgtgttg gttaacgtga actcgcgcgt cgaataggcg
     9601 atggttcc
//
LOCUS       DASTKU010000436         8114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1867127, whole genome shotgun sequence.
ACCESSION   DASTKU010000436 DASTKU010000000
VERSION     DASTKU010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867127"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1891
                     /locus_tag="VFQ61_14930"
     CDS             <1..1891
                     /locus_tag="VFQ61_14930"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HET9955802.1"
                     /translation="ITHFDHERIPERVVHARGTGLHGHFTLTHSLAKYTRAKVLTEVG
                     EKTPVFMRLSTVAGGAGSVDTPRDVRGFAVKFYTKEGIWDFVGNNIPVFFVQDAMKFP
                     DFVHAVKMEPDRGFPQAASAHDTFWDFISLTPESMHMIMWAMSDRTIPRSLATIEGFG
                     IHSFRLVNDAGESTFVKFHFRPKAGLQSTLWDEAVKIAGADPDFHRRGLFEDIQAGKY
                     PTWEFAVQLFTEQEAESFPFDHLDPTKIIPEELVPLMVIGELVLDRWPDNFFAETEQV
                     AYCPANVVPGIDFSSDPLLQGRLFSYLDTQLSRLGSPNFHQLPINAPKCPFANHQRDG
                     HMQSAVPKGRVAYEPSALDPSGARQSRAGFRSARVKNEPGEKGRIRPESFADHYSQAR
                     MFYRSQTEYEQAHIVAALTFELSKVDTPEIRSRIVAHLRNIDESLANRVAKGLGLDML
                     PDAPDPAAPIQDLPLSPALRVIGKMKDTFEARVVGIVVADGSDGAVVEALRGAVQGAG
                     GRVKVIAPRIGGVTMIDGSHVPADGQLAGNPSVFFDAVAIVLSDKAGESMSRQAAALD
                     FVRDAYSHLKAIAVDTGGALLLRAAGIEQDQDAGIVAASDTVAFIQVAKTRQWDREEL
                     VRPST"
     gene            complement(1961..2488)
                     /locus_tag="VFQ61_14935"
     CDS             complement(1961..2488)
                     /locus_tag="VFQ61_14935"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET9955803.1"
                     /translation="MPSIGHVAVGLAAARLASSTAGIPVPRIRLALLLSAAALLPDLD
                     AIGFELGVPYGAPWGHRGAAHSLVFAAIVGVLVAPLGRQFKLSSAQLWLWVTCATASH
                     GLLDSLTNGGRGIALLWPFTHARFFAPWRPIAVAPLGLRVLSARGVYVVGQELLLFAP
                     LFIYGLWPRRGNKPS"
     gene            complement(2601..3563)
                     /locus_tag="VFQ61_14940"
     CDS             complement(2601..3563)
                     /locus_tag="VFQ61_14940"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9955804.1"
                     /translation="MNRTTLGCVSIVLSAGLLSPALAQADPPTDAANADATEAARAHF
                     NQGLRLYKDGDFDAALVQFERAYTAKPNYRVLYNVAQTYFQLRQYVESRDAMIQYLKE
                     GGEAIERERRESVNRDLADLERRIAKVTILVNVNGAIVFVDGRKVGTTPLSEPVRVSE
                     GQRTISIESPQRGTRQRLIRVAGGEEQSLTLTFEAPSVTPSVTPASLSPESGSQLSTG
                     FWLTALGSVTLAAGAGVTGLLALRAQAENRDQRESWGASASSLQDSRDRAKALALTTD
                     VLGGAAVICAGSAIYLYFSSTPRQEPAKLSVGLGPSSAELTGSF"
     gene            complement(3579..5255)
                     /locus_tag="VFQ61_14945"
     CDS             complement(3579..5255)
                     /locus_tag="VFQ61_14945"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9955805.1"
                     /translation="MSSLVPESSASLGKYRLLLELGAGGMARAYVAFSSGLAGFHKLV
                     VLKVMREEVNDHQDTLAMFLDEAKLAARMNHPNIVQTLEVGEDRGKYFICMEFLEGLT
                     LGRLLKRTFAEPLPLGARLELMSQVLDALIYMHEFRDLDGTPLHLVHRDLSPNNVFVT
                     FDGTAKVLDFGVAKAAGVSRATEAGTFKGKFGYAAPEQMIGHADQRADLFAMGVMLWE
                     TTTGERLHHGRTQQEIVQGRLAGVDAQLMRRASSIPEPLLEICLRAAAREPDARFSSA
                     SEMRDALRACIKREKLEFSSEELRTVLSRLFASERAEIRRTIDVRMKQAQSDGGEPGT
                     FTHSSAIPKAPAVFGPSGTMTGLLSTGGGWQTRPPAPSNAKWIALGIAAAALAAVAGA
                     LLTRTSPPLQAHVVNAAPISKAPPSPEGPAVKKAELVSLKVNATPLDAQIYLDGARLE
                     GNPFNGLLPKDPGLHRLEVRHESGRSEARMIALTRDQELTVDLRVRSIAAHVAPAAHR
                     SGPSPKSADLAVAVSPAPNPTPALAPGDGLTRRKPSENARPIDDSDPYTH"
     gene            complement(5252..6184)
                     /locus_tag="VFQ61_14950"
     CDS             complement(5252..6184)
                     /locus_tag="VFQ61_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955806.1"
                     /translation="MLETSMTLGNSHSSKPGLRRGLIHSFVRSQSARRTRIARGSTAI
                     LALIFSLSSGCSAIINVEEAQCQTDADCGQLGPEFMNSVCQAQVCVPSSSSQLECEDP
                     IQSSDTIKYTFTPKYATPQESPAPFTVMACERLDATCTKPVAELEGVVAGELQELTLP
                     WGFSGFFRYENPKTHTALHFLGRPLKQDTRGWDLTIADDTTVAVLGISTNTTIDPALG
                     TMIVIARDCEGRPLEHFKFTNATGGTPFFIVDNFPTPDATETTEQGSGGFVNVKLGTT
                     VVSAQNASGAMLDGTQAILKPIGERWVSYVEIFP"
     gene            complement(6662..6820)
                     /locus_tag="VFQ61_14955"
     CDS             complement(6662..6820)
                     /locus_tag="VFQ61_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955807.1"
                     /translation="MNSAVSAQVQYAANGKHGPFIVATMRTSSGKRTSSGKRMASERF
                     LLVNTLRQ"
     gene            7000..7905
                     /locus_tag="VFQ61_14960"
     CDS             7000..7905
                     /locus_tag="VFQ61_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955808.1"
                     /translation="MAAPREIIPGRYYMITRRCVRRQFLLRPDQRTNQCFLFCLALAA
                     QYSGVRVVCFLAMSNHYHAIVHDPLGTLPLFLEHLHKLCAKALNARWSRWENLWATEQ
                     TCATHLVNPSDVLDKVLYTLCNPLAEHLVERVTDWPGANSYRAMLTGRTIEVERPRDF
                     FREHGSTPDVARLTLARPPGFEDWDQPKWEEYLTGLVTGIEERTRRERLERGIKLRGR
                     KAVLRASELDTPDGAEPRRKLRPSVACADAVARTRVLTQLVLFRRAHEVARLRVAAGE
                     LEAVFPYGTYRMRLMGHRCARAPSD"
BASE COUNT         1485 a         2402 c         2686 g         1541 t
ORIGIN      
        1 gatcacccac ttcgatcacg agcgtattcc cgagcgcgtg gtgcacgcgc gcggtacggg
       61 attgcacggc catttcacgc tcacgcactc actcgccaag tatacgcggg ccaaggtttt
      121 gaccgaagtt ggggagaaga caccggtgtt catgcggctc tcgacggtgg ccggcggcgc
      181 gggatcggtg gacacgccgc gtgacgtgcg cggcttcgcg gtgaagttct ataccaagga
      241 ggggatttgg gacttcgtgg gcaacaacat ccccgtgttc ttcgtgcaag atgccatgaa
      301 gttcccggac ttcgtgcatg cggtgaagat ggagccggac cgcggcttcc cgcaggcggc
      361 gagtgcgcac gacacgtttt gggacttcat ttcgctgacg cccgagtcga tgcacatgat
      421 catgtgggcg atgtccgatc ggaccatccc gcgctcgctg gcgacgatcg agggattcgg
      481 aatccatagc ttccgactcg tgaatgatgc cggtgagtcc acgttcgtga agttccactt
      541 tcggcccaag gccggtttgc agtcgacctt gtgggacgag gccgtcaaga ttgcaggcgc
      601 ggatccggac ttccaccgac gggggctatt cgaggacatc caagccggga agtacccaac
      661 ctgggagttc gcggtgcagc tcttcaccga gcaggaagcc gagtcgttcc cgttcgacca
      721 tctcgatccg accaagatta tccccgaaga gttagtgcct ttgatggtga ttggcgagct
      781 ggtgctcgat cgttggccgg acaacttctt cgccgagacg gagcaggtgg cttattgtcc
      841 ggccaatgtc gtgcctggga tcgacttctc gagtgacccg ttgttgcaag gccggctgtt
      901 ctcgtacttg gatacgcagc tctcgcgact cggctctccg aatttccatc agctccccat
      961 caatgcgccg aagtgtccgt tcgccaatca tcaacgagac gggcacatgc agagtgcggt
     1021 tcccaagggg cgcgtagcgt acgaacccag cgcgctcgat ccgagcggcg cgcgtcaatc
     1081 ccgagctggt ttccgcagcg cgcgggtgaa gaacgaaccc ggggagaagg ggcgcattcg
     1141 tccggagagc tttgcggatc actatagcca ggcccgcatg ttttaccgca gccagactga
     1201 gtatgaacaa gcgcatatcg tggcggcgct caccttcgag ctctcgaagg tcgacacgcc
     1261 ggagattcga tcgcgcatcg tcgctcacct ccgaaacatc gatgagtctc tcgccaaccg
     1321 agtcgcaaaa ggcctcggat tggatatgtt gccagacgct cccgacccgg ccgcaccgat
     1381 tcaagactta ccgctgtcac ccgcgctgcg cgtgattggt aaaatgaaag atacgttcga
     1441 agcgcgggta gtcgggatcg tggtggcgga cggatcggac ggagcggtgg tggaggcgct
     1501 gcgtggcgcc gttcaaggag cgggaggccg ggtaaaggtg atcgcgccac gcattggcgg
     1561 ggtgacgatg atcgatggca gccacgtacc cgccgacgga caactcgcgg gtaatccctc
     1621 tgtctttttc gatgcagtcg cgatcgtgct gtccgacaag gcgggagaat ccatgtcgcg
     1681 tcaagcggcg gcgctggact tcgtgcgaga tgcgtattca cacttgaagg cgattgcggt
     1741 cgatacgggc ggggcgctgc tgctacgcgc agccggcatc gagcaggacc aagacgcggg
     1801 catcgtcgcg gcgagcgata cggtggcgtt cattcaggtc gcgaagacac gacaatggga
     1861 tcgcgaggag ctggtgagac cgtcgacata gcgttcctct tcgacgatgc agctcattcg
     1921 tgggccaagg ctcgtcacgg caccggggtc cgtgtcttcg tcacgacggt ttgtttcctc
     1981 gtcggggcca aagaccgtag atgaagaggg gtgcaaagag caggagctcc tgacccacga
     2041 cgtacacccc gcgggcactc agcacgcgca gcccgagggg tgctacggca atcggccgcc
     2101 aaggtgcgaa gaagcgagcg tgggtgaatg gccaaagcaa ggctattccc cggccgccgt
     2161 tggtgagcga gtccaacaag ccatgactcg ccgtcgcgca ggtcacccag agccacagtt
     2221 gcgcgctcga gagcttgaat tgccggccca ggggggcgac gagtacgccc acgatcgcgg
     2281 cgaaaaccag ggaatgggcg gctccgcggt ggccccaagg cgcgccgtat ggaacgccga
     2341 gctcgaaccc gatcgcatcc aagtcaggca gtaatgcggc cgcgctcagc agcagggcga
     2401 gccgtattcg aggcacggga atgcccgcgg tggagctcgc gaggcgtgct gccgccaggc
     2461 cgaccgcgac atgtccgata cttggcattg gagatctatg ccacgaactc gtgtatctcc
     2521 gccaagaacc agaaccgctg acgagttcac gtgctgctca cgcgcttcgg acgcggcgcc
     2581 aagcgcgtga gcaggggtgc ctaaaagctg ccggttagct ctgcggagct cggacctagg
     2641 cccacgctca gtttggctgg ttcctgccgc ggcgtgctcg aaaagtataa gtagattgcg
     2701 gagcccgcgc agatgacggc tgctccaccg agcacgtcgg tggtgagcgc tagcgctttg
     2761 gcccgatcgc gggagtcttg gagactgctc gcactcgcac cccaactctc gcgttggtcc
     2821 cggttctccg cttgcgcgcg taacgcgagt aggccggtga cgccggcgcc ggcggccagc
     2881 gtgacggagc caagggccgt gagccagaat cccgtgctca attggcttcc gctctcgggg
     2941 gacagggacg cgggggtcac ggaaggcgtg acggaggggg cttcgaatgt caaggtgagg
     3001 gattgctctt ctccacccgc aactcggatg agtcgctggc gggtgccgcg ttggggcgac
     3061 tcgattgaga tggtgcgctg gccctcgctc acccgaaccg gttcgctcag gggcgtggtg
     3121 ccgactttcc tgccgtcgac gaagacaatg gcaccattca cgttgaccaa aatcgtaacc
     3181 ttggcgatgc gacgttctag atccgccaga tctcgattga cggactcacg gcgctcacgc
     3241 tcgatggctt cgccgccttc cttgagatat tgaatcatcg cgtcgcgcga ctccacgtat
     3301 tgacggagct gaaagtaagt ctgcgctacg ttgtagagaa cccgatagtt cggtttcgca
     3361 gtgtaagcgc gctcgaactg caccagcgcg gcgtcgaagt cgccgtcctt gtagaggcgc
     3421 aagccctggt tgaaatgggc gcgtgcggcc tccgttgcgt ccgcgttggc cgcgtccgtc
     3481 ggtggatcgg cctgggccaa agctggtgag agcaggcctg cactcagaac tatcgagacg
     3541 caaccgagag tggtacgatt catggctctt cgtcggagtt agtgagtgta gggatccgag
     3601 tcgtcgatgg ggcgagcgtt ttcgctcggt ttgcgacgcg tgagcccatc cccaggcgcc
     3661 agcgcgggcg tggggttcgg cgcaggggac accgccacgg ccaagtctgc gctcttggga
     3721 ctcgggccgc tgcgatgagc ggcgggggcg acgtgagctg caatcgagcg aacgcgaaga
     3781 tcgacggtca gctcttgatc tcgagtgagc gcaatcatgc gcgcctcgga tcttccggac
     3841 tcgtgccgta cctccaggcg gtgaagcccg ggatccttgg ggagcaggcc gttgaacgga
     3901 ttgccttcga gtctcgcccc gtccaagtag atctgcgcgt cgagcggcgt cgcgttcact
     3961 ttcaggctga ccagctcggc tttcttgacg gcgggcccct ctggcgatgg cggcgctttc
     4021 gagatcgggg cggcgtttac cacgtgagct tgcaggggcg ggctcgtgcg ggtcaaaagg
     4081 gcaccggcaa cggctgccag cgccgccgcg gcaattccga gcgcgatcca cttggcgttg
     4141 gaaggggcgg ggggccgcgt ctgccaaccg ccccccgtcg agagcagccc cgtcatcgtg
     4201 cccgacggac cgaacaccgc cggtgccttg gggatagcgc tcgagtgcgt gaaggttcct
     4261 ggctcaccac cgtcgctctg cgcctgcttc atgcgcacat cgatggtgcg gcggatctcg
     4321 gctcgctcgg acgcaaaaag ccgactgagc accgtgcgca gctcttcact cgagaactcg
     4381 agcttctcgc gtttgataca cgcgcgcagt gcgtctcgca tttctgatgc ggagctgaaa
     4441 cgagcgtctg gctcgcgggc ggccgcgcgc agacagatct cgagtagcgg ctccgggatg
     4501 ctcgaggcgc ggcgcatcaa ttgtgcgtcc acacccgcca ggcgaccctg aacgatctct
     4561 tgttgggtgc ggccatggtg caggcgctca cccgtggtgg tctcccagag catcacgccc
     4621 atcgcgaaca gatcggcgcg ttggtcggcg tgcccaatca tctgctcggg cgcggcgtag
     4681 ccgaatttgc ctttgaaggt gccggcctcg gtcgcgcggc taacccccgc ggctttggct
     4741 acgccgaagt cgagcacttt ggcggttccg tcgaaggtga cgaagacgtt gttcgggctg
     4801 agatcgcgat gcaccaggtg caacggtgtt ccgtcgagat cgcggaactc gtgcatgtag
     4861 attagagcgt cgagcacttg gctcatcagc tccagtcggg cgcccagggg gaggggctcc
     4921 gcaaaggtgc gcttgagcag ccgccccagg gtcaacccct ccaggaactc catgcagatg
     4981 aagtacttgc cgcggtcctc gccgacctcc aaggtctgca cgatattcgg gtgattcatg
     5041 cgcgccgcga gcttggcctc atcgagaaac atggccagcg tgtcttggtg atcgttcact
     5101 tcttcacgca tcaccttcaa cacgacgagc ttgtggaagc cagcgagccc ggatgaaaag
     5161 gcgacgtaag cgcgggccat gccacccgcg ccgagctcga gcaagagtcg gtatttgccc
     5221 aggctggcgg aggactccgg gaccagagaa ctcacgggaa tatctccacg tacgagaccc
     5281 atcgctcgcc aattggcttc agaatggctt gcgtgccgtc gagcatcgcg ccggacgcat
     5341 tctgggcgct caccaccgtc gtgccgagtt ttacgttcac gaaaccgccg cttccttgct
     5401 cagtagtttc ggtggcgtcg ggagtgggga agttgtcgac gatgaaaaac ggcgtaccgc
     5461 cggtcgcgtt ggtaaacttg aagtgctcga gaggcctgcc ctcgcagtca cgcgcgatca
     5521 caatcatcgt tcccagggcc ggatcgatcg tcgtgttcgt ggagatcccc aatacggcca
     5581 ccgtcgtgtc gtcggcgatg gtcaaatccc aaccccgagt atcttgcttc aggggacgcc
     5641 ccagaaagtg cagggcggtg tgcgtcttgg gattttcata gcgaaagaag cccgagaagc
     5701 cccagggcag agtcagctct tgaagctctc ccgcgactac accctcgagc tcggcgacgg
     5761 gcttggtgca cgtcgcatcg agccgctcac acgccatgac ggtgaacgga gcagggctct
     5821 cttgaggagt cgcgtacttg ggcgtgaaag tgtacttgat cgtgtcactg gactgaatgg
     5881 gatcttcgca ctccagctga ctcgagctgc tcggcacgca cacctgcgcc tggcataccg
     5941 aattcatgaa ctccgggccg agctgaccgc agtcggcgtc cgtctgacac tgggcttctt
     6001 cgacgtttat gatagcggag catcccgaac tcaggctgaa gatcagcgcc agtattgcag
     6061 tggaaccgcg cgcgatgcgc gtacgccgag cggactggct ccgaacgaag gagtgaatca
     6121 gaccgcgtcg cagccctggc ttggatgaat ggctattgcc caaggtcatg gaggtttcga
     6181 gcactctctg agctacggcc cgggaggcga agactcgtat gctcattagc agaaaaacga
     6241 tcgaaagtga ctcgttccca tccgatcgaa tgggtagctc cgacgcccac ccggcgccga
     6301 tcattggaca gccgcaccgt gaacccgttg cagacatcaa tcgaagggtt tggtgctcga
     6361 gagggcgcct ccagtgaaac agtctgagac gcggcggcca tcgggaagcc gcggaaaggg
     6421 cagctcggga acgggtctgc cgctcttgga atctgcctaa atgttgcggg acacgggacg
     6481 agcgtgggag ctcctagagg ccggcggata cttgcgcgag cgccagtcgc ggtgcgtctg
     6541 gggtggccgc ctcggtttcg cgagatagtg cgcctcgcgc ggacgtggga ccgggccgcg
     6601 ccgcgtggat tggtcaatga ccgcggcggc ctcgactggt gacgggtcgt tctcgagaag
     6661 atcactgacg tagcgtgttc acgagtaaaa atcgctccga agccatgcgc ttgccactac
     6721 tcgtccgctt gccactactc gtccgcatcg tcgcaactat gaaggggccg tgtttgccgt
     6781 tggcggcata ctggacttgc gcggagacag cgctgttcat ttacgtacca agctgcttgc
     6841 ggagatcggc cgaacgagcc agtcggctag ggtgctcata ggcatatcct atcgatttga
     6901 catgattggg ctgccattca ccagcggcgg ccggggcttc gagtgcagta ttcggcggac
     6961 tattcggttt gtacacgcct tgcactgcaa ggcggcacct tggcagctcc gcgcgagatc
     7021 attcctgggc gctattacat gatcacacgt cgctgcgtac ggcgacagtt cttgctacgc
     7081 ccggaccagc ggacgaacca gtgcttcctt ttctgtctcg cactggctgc gcagtactcg
     7141 ggagtgcggg tcgtgtgctt tttggcgatg agcaaccact atcatgcgat agtgcatgat
     7201 ccgctcggta cactaccgct cttcctcgag cacttgcaca agctttgcgc caaagcattg
     7261 aacgcgcgat ggtcgcgttg ggagaacctt tgggccaccg agcagacctg cgcgacccat
     7321 ctggtgaatc cgagtgacgt actcgataag gttttgtaca cattgtgcaa tccgttggcg
     7381 gagcacttgg tcgagcgagt gacggactgg ccgggcgcca actcgtaccg agctatgctc
     7441 acgggacgaa ctatcgaagt cgagcgtcca agggattttt tccgagagca cgggagcacg
     7501 cctgatgtcg cgcgactcac tttggctcgc cctccgggat tcgaagactg ggatcagcct
     7561 aagtgggagg aatacctgac cgggctggtg acggggatcg aagagcgcac acgaagggag
     7621 cggctcgaga gggggatcaa gcttcgcgga cggaaggcgg ttcttcgagc gtctgagctc
     7681 gatactcctg acggggcgga gccgcggcgg aagctgcgac cgtccgttgc ttgcgccgat
     7741 gccgttgctc gtacgcgagt gttgacgcag ctggttttgt tccggcgggc gcacgaagtg
     7801 gcgaggttgc gagttgcagc gggggagctc gaggcggtgt tcccgtacgg tacttatcgg
     7861 atgcgtctga tgggccatcg ctgcgcgagg gcgccttcgg actagcgctc gagctctgag
     7921 ctgagctgag ccgtcagccg ggcggtcgac gggagtagtg cgccatgcgg cgggacgtcg
     7981 cgcgggaggg gagaggtgtg cccgcgaaag gtggccgagg gggagtcgtc tgagtgcctt
     8041 cggcacggga gggcccgtcc cgcacctcgc ccgcgaccca cctcatcatc ctgccaactc
     8101 cgcaacgtgg tacc
//
LOCUS       DASTKU010000437         7370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1421615, whole genome shotgun sequence.
ACCESSION   DASTKU010000437 DASTKU010000000
VERSION     DASTKU010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7370
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421615"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VFQ61_14965"
     CDS             complement(<1..1305)
                     /locus_tag="VFQ61_14965"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955809.1"
                     /translation="MPPRTLARPFSFSPGVWATLPLLVLVCHCTDFVIDPPVPGGAAG
                     TGSGGGPQGGGGGGAGGFSGGTTGGERAQMPALTDDVYTLLQGETLTVSAAEGVLSND
                     RPLKLRVRGVPRLISSVPSQFTGQLVLEEDGAFVFQPEPRFFGVYGLEYTAENESGQS
                     APAELWFRVLPERISLDVIESGLGGYVLRGSEAGHFGAALAPAGDLNGDGLADLLVGA
                     PDTAQGKGAAYLVYGKEDLSPVDVLSNAPSSATGVYTAFSAGAGETLGVSVAGLGDVD
                     RDGLPDLGLGANGANAGAGRVYVVAGRRTFARQFALPDPGGMVIEGDAGNQDVGRVVA
                     GAADVDGDGQRDILINSNNLKYGWLHVLFGGTGVTGAVPIFGLAGLHLRAAVEEDLFP
                     TSAAAIGDFNGDGASEILASSQRNIVVVEGGETYPDHIGALPI"
     gene            complement(1305..2462)
                     /locus_tag="VFQ61_14970"
     CDS             complement(1305..2462)
                     /locus_tag="VFQ61_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955810.1"
                     /translation="MNLVRPRFSVCPRFGEARSKLARVPRNGARSAPRAAANLLIRAS
                     AEWLPLAALLCAMTLGVTGARAQPSEDLTHGVSLELVHEGNGFWASGDYLSAADRYDR
                     AYEIERAKVLQLWAARALERAGKWVEAYERYTMVTELPAEATSQDWDAARSARRDAAR
                     LEQQIPRLTVRVEGAVARDVVVRLNGEILSAAQIGVARYANPGLVRVTGAAADARRTA
                     REEKAVLTPRANVVLTLKFRPARHLESMNGTSSGAPAGASISADTRRTLAYVSFGVSG
                     AALLTGFTFAALALKDQADLKERCGGRHCPYELASDVDSFESKRTVSVATLIAGGVFA
                     GGGLALYLTVPRSAAPAGQSNPRARASGHVDGRALWEVGWVGSGGYVRGSF"
     gene            complement(2613..3152)
                     /locus_tag="VFQ61_14975"
     CDS             complement(2613..3152)
                     /locus_tag="VFQ61_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955811.1"
                     /translation="MASAPSAGFRVVRKHGRIELGKLAVVGALACALLAVSSAYGAGP
                     AKNGAEPRAEVTFSGFRDLGAGRAIVFVELTDRVEVSVSQKGTEVVYELKGAVVRLKN
                     NQNPLVTSAFSSNLLSARLTPIGKMQKAPKAKAAKPRDKSTAQGVQLILKLRQPVVPA
                     YKFIERSTGVVFEITIPAG"
     gene            complement(3311..4249)
                     /locus_tag="VFQ61_14980"
     CDS             complement(3311..4249)
                     /locus_tag="VFQ61_14980"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9955812.1"
                     /translation="MQDPRAQHPRTQDSHSEVASPFPLTALSDQLSALAQKAESSLVR
                     VEGRRGSVSSGVAVGPGLVLTAAHGLGDAERVTVSWGERTESAERLYVQPEADLALLR
                     LEAQLPTLAPAEVASLRVGELVLAVGRPGDGVSARLGVIAALGEAWRLPGGLRLERYI
                     ETDIGAAPGFAGGAILRANGEWVGLINSHASRTSLVALPAPSVLRFVEALRSAGGQHR
                     PRLGVSVQGVKLPSEWVQRFGRECGLLVMSVLPGSPAARAGVLLGDVLLAAGSAPLAC
                     ATELECALFEAAGEREQAFEVLRGGELRKLLARFDA"
     gene            4248..4994
                     /locus_tag="VFQ61_14985"
     CDS             4248..4994
                     /locus_tag="VFQ61_14985"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9955813.1"
                     /translation="MMSLLDKLQRRPIPPADWAGRTYLFGQVVIVALYNSSVELKFDR
                     MLALTFAAAIIAESALVRSGLAAILGASPEIRVVAEATPAEASSLPTEGVDVVVCDIA
                     DGARAEAIMEQAPRGVPVLALVGRPEATRALVRAGVKGVIARDASSERLSAASVAVAT
                     GLCTLDEQTLQQLVSPTPAGQPQTLLTPREQEVLELLAEGLSNKLIGLRLDISEHTAK
                     FHVNSILEKLEADTRTDAVVRAARRGMLTL"
     gene            complement(5024..6226)
                     /locus_tag="VFQ61_14990"
     CDS             complement(5024..6226)
                     /locus_tag="VFQ61_14990"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9955814.1"
                     /translation="MPQYRTAVGDRSLVADLEYPYYLAYAAISPATRAVRDALHESIE
                     DYARVGQLAIGRTIEQRERLRGKVARLLNARAEEIGLTTGTTHGLLQLAFCIDWKPGD
                     RVVVFRGEFPANFTPWQNAAACFDLELCELQALGASVGGRDRSQDADLSELETTLRGG
                     GVRLVAISAVAFQTGYRAPLRAVAELCHAHGAELCVDLIQACGVVPVDVRASGIDYAA
                     GGAHKWLMGAQGAGFLYARGELQRLLIPRMAGWLSHEDGVRFLFEPGQLRYDRPLKRS
                     IDFVEGGSQSALSSVAMEAGLDPILELGVPAIFSHVSAYLDRLESPLMDRGFESLRST
                     SVGARSGMLCLVPPEGKTAAELVSSLRAHGVVASMPDGNLRFAPQFTNSVEEVPSVLE
                     AVDAALAR"
     gene            complement(6347..>7370)
                     /locus_tag="VFQ61_14995"
     CDS             complement(6347..>7370)
                     /locus_tag="VFQ61_14995"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET9955815.1"
                     /translation="IPAFGASPIYTVDTNAVQAYKEAQLESGFSRKTINNHLVVLRAV
                     FSLAIAREIIGAAPQIQSFALEAPDLQFLGFDEARALVRAADPDWRLMIILGLRTGLR
                     LGELRALTWDNVDLEHLRLRVQQRSRDRLDENVRSREIPLSEDTAAELRQHPRRGKWV
                     FCTEEGEMLTHGACKWPLYRAARRAGLRQLGWDVLRRTFAAHLTLRGAPLASVQALLG
                     HRSMSTTLRYTQLGGQGTRAAIALLDVKDPGAGPAASGASSREGAGNSERDRARFGWR
                     PLPGWFDPAQLESVLRPEELASGAVLPKPVAANPIVAAGTSTRNRSYPRLRHPALDRP
                     APCIHC"
BASE COUNT         1398 a         2505 c         2243 g         1224 t
ORIGIN      
        1 gatcgggagc gcgccgatgt gatctggata cgtctctccg ccctcgacca cgacgatgtt
       61 ccgctgcgat gaggccagga tctcgctcgc gccgtcgcca ttgaaatcgc caatcgccgc
      121 cgcgctcgtc gggaacaggt cttcttcgac cgcggcccgc aaatgtagac ccgccaggcc
      181 aaagatgggt acggctccgg tcacgccggt tccgccgaag agcacgtgga gccacccgta
      241 cttcaggtta ttcgaattga tcaggatgtc ccgctgcccg tccccgtcga cgtctgcagc
      301 tccggccacc actcgaccga catcctggtt gcccgcgtcg ccctcgatca ccatgccgcc
      361 ggggtcgggt aaagcgaact ggcgagcaaa cgtgcgacgg cccgcgacga cgtagacgcg
      421 accggcccca gcgtttgccc cattcgcccc gaggccgaga tccggcaggc catcccgatc
      481 gacgtctccc aaacccgcca ccgacacgcc cagagtttcc ccggcgcctg cagagaacgc
      541 agtgtagacc cccgtcgccg agctcggcgc attgctgagc acgtcgaccg gcgacagatc
      601 ttctttgccg tacaccaagt atgcggcccc cttgccctgg gccgtgtccg gcgcgcccac
      661 gagcaagtca gccaggccat cgccgttcag gtctcccgcg ggcgcgagtg cggccccgaa
      721 atggcctgct tcggagccgc gcagcacgta accccccaaa ccactctcga tcacgtccag
      781 cgagatgcgt tcgggcaaaa cgcgaaacca gagctcggca ggcgcgcttt gaccgctctc
      841 gttctctgcc gtgtactcga ggccgtacac tccaaagaag cgcggctcgg gctggaagac
      901 gaatgccccg tcttcttcca aaacgagctg acccgtgaat tggctcggca ccgacgagat
      961 caagcgcggc acccctcgga cccgaagctt caaaggtcga tcgttggaga gcacaccttc
     1021 cgccgccgag accgtcagcg tttcaccttg cagcaatgta taaacgtcgt cggtcaacgc
     1081 tggcatttgc gcgcgttcgc cgcccgtagt gccgccggaa aagccaccgg ctccaccgcc
     1141 gcctccacct tgcggcccgc caccactgcc ggtgccggca gcgcctccgg gtaccggagg
     1201 atcgatgacg aagtccgtgc agtgacacac gagtaccagc agcggcagcg tggcccagac
     1261 tccgggtgaa aacgagaaag gacgagcgag cgtgcgcggg ggcatcagaa gctcccccga
     1321 acatagccgc ccgagcctac ccagccgact tcccagagcg cgcgtccatc gacgtgcccg
     1381 gaggcgcggg cgcgagggtt gctttggcca gcgggcgccg ctgaacgagg gacggtcagg
     1441 tacagtgcca accctccccc ggcgaacact ccacccgcga ttagcgtcgc gacgctcacc
     1501 gtgcgcttgg attcgaacga gtccacgtcg gaagcgagtt cgtacgggca atggcgtccc
     1561 ccacagcgct ccttcaggtc agcctgatct ttcaaggcca gcgccgcaaa ggtaaacccg
     1621 gtgagtagcg cggcgccgga cactccaaag gatacatagg ccaacgtgcg tcgtgtatcc
     1681 gctgagatgg atgcgcctgc gggagctccg ctcgaggtgc cgttcatgct ttcgagatgg
     1741 cgcgcgggtc gaaacttgag cgtgagcacg acgttcgctc gcggagtcaa caccgccttc
     1801 tcttctctcg ccgtgcgccg cgcatctgcc gccgctcccg tcacccgaac caatccaggg
     1861 ttcgcgtagc gggcaacccc gatctgagcc gccgacagga tttcgccgtt gagccgcacc
     1921 accacgtcac gcgcaaccgc accctcgacg cgaacagtga ggcgtgggat ctgctgttcg
     1981 aggcgagccg cgtcacgccg agcgctgcgc gcggcgtccc agtcttggct cgtggcttcg
     2041 gctggaagct ctgtcaccat cgtgtagcgt tcgtacgctt cgacccactt tccggcccgt
     2101 tccaaggcgc gcgcggccca gagctgcaga accttcgcgc gctcgatttc ataggcacga
     2161 tcgtatcggt ctgccgccga taagtagtca ccgctcgccc agaacccgtt tccttcgtga
     2221 acgagctcga gcgagacgcc gtgggtcaaa tcttccgaag gctgcgctcg tgccccagtc
     2281 acccccaaag tcatcgcgca cagtagggcc gccaacggca accactcggc agacgcgcga
     2341 atcaagaggt tcgcggcggc gcgcggcgcg ctccgcgctc catttcgagg gacgcgagct
     2401 agcttggacc gcgcctcgcc aaagcgaggg caaacactga accggggcct gacgaggttc
     2461 acgctctggc ccttctatcg caaaattagg ccgagcatcc tgaatttggg ccacgctcct
     2521 caaattcagg cacggacagg agcgcgcgtt cgagagaaca tgaggcagct gctgctcctc
     2581 cacgtccaaa ctcgaaagca cagacgcgag agtcagccgg ccgggatagt tatttcgaag
     2641 accacccctg tagatcgctc gatgaatttg taagcgggca ccaccggctg acgcagcttc
     2701 aggatgagct gcacgccttg cgccgtagac ttgtcgcgtg gcttcgcagc ctttgccttg
     2761 ggagcctttt gcatcttgcc aatcggggtc agccgcgcgc tgagcagatt ggacgagaac
     2821 gcactagtta ctagcgggtt ctggttgttc ttcagacgca cgacagctcc cttgagctcg
     2881 taaaccacct cggtgccctt ttggctgacg ctgacctcta cgcgatcggt caactcgacg
     2941 aacacgatcg ctcgcccagc gcccagatcg cgaaagccgc tgaatgtgac ctcggcacgg
     3001 ggctccgcgc cgtttttcgc agggccggcg ccgtacgcgc tcgagaccgc aagcagcgcg
     3061 cacgccaacg cgcccactac ggcgagctta ccgagctcaa ttcggccgtg cttgcggacg
     3121 actcgaaagc cagctgaggg ggcggacgcc atggcacgac gttacccgga attctggctg
     3181 agctcatgca ctttcgagct gcctggcccg gacacggaaa gggtggtcag gtggtttggt
     3241 gagcgccgga aggatcattc gtgccgcccc cgagccctcg gcagaccgtg tgcccttcac
     3301 tgggcggggt tcaggcgtcg aagcgagcca gcagcttgcg cagttctccg ccgcgcagga
     3361 cttcgaacgc ctgctcgcgc tcacccgcag cctcgaagag tgcgcactcg agctcggtcg
     3421 cgcacgcgag cggcgccgac cccgcagcaa ggagtacgtc cccgagcaac acgcctgcgc
     3481 gagccgcagg gctccccggg agcaccgaca tcacgagtaa gccgcattcc cggccgaagc
     3541 gctgaaccca ctcgctcggt agcttcaccc cctgcacgct cacgcctagt cgaggccgat
     3601 gttgcccgcc cgcgctacgc agggcttcca cgaaacgcag cacactgggg gccggcaagg
     3661 ccaccaagct cgtgcgtgac gcgtgggagt tgatcaatcc aacccattcc ccgttggccc
     3721 ggagaatggc gccgcccgca aacccagggg ccgcaccgat gtcggtttcg atgtagcgct
     3781 ccaagcgcag cccaccgggc aaccgccaag cctcccccaa cgcggcgatg acgccgaggc
     3841 gagcgctcac gccgtcgccg ggacggccta cggcgagcac gagctcacca acccgcaagc
     3901 tcgcgacttc ggcgggagcc agcgtgggga gctgcgcctc caagcgcagc aacgcgaggt
     3961 cagcctccgg ctgtacatag agccgctcgg cgctttcagt gcgctctccc caagatactg
     4021 tcacgcgctc cgcatcaccc aggccgtggg cagccgtaag aacgagaccc gggcccaccg
     4081 ctacaccgct cgacaccgac ccacgtcgcc cctccacgcg gaccaagctc gactccgctt
     4141 tttgagccag cgcgcttagc tgatcggaca gcgcggtgag cggaaacggc gacgcgactt
     4201 cagaatgcga atcttgagtg cgtggatgtt gcgcgcgggg gtcttgcatg atgagcctat
     4261 tggataagct tcagagacgc ccgatcccac ccgcggattg ggccggtcgc acctaccttt
     4321 ttgggcaggt cgtgatagtc gccctttaca actcctcggt cgagcttaag ttcgaccgaa
     4381 tgttggcgtt gacctttgcc gcagccatca ttgccgagtc tgccctggtt cgtagtgggc
     4441 tggccgcgat tttgggtgct tccccagaaa ttcgggtggt tgcggaggcg acgcctgcag
     4501 aagcaagtag cctgcccacc gagggtgtgg acgtcgtggt ctgcgacata gcggatggcg
     4561 cgcgcgcgga ggccatcatg gagcaggccc ctcgcggcgt gccggtgctg gcgctcgtcg
     4621 gccgcccgga ggcaacccgt gccttggtcc gtgccggggt caaaggggtc attgcgcgag
     4681 acgcttcgag cgaacggctg tccgccgctt cggttgcggt cgccacaggg ctatgcaccc
     4741 tggatgagca gacgttgcaa caattggtct cccccacccc cgcaggccaa ccgcagacgt
     4801 tgctgacgcc ccgcgagcaa gaggttttgg agctcctcgc cgagggccta tcgaacaagc
     4861 taatcggttt acggctcgac atctccgaac atacagccaa attccacgtc aactcgatac
     4921 tcgagaagct cgaggcagat acgcggacgg atgccgtagt tcgagcagca cgccgcggca
     4981 tgctgaccct ctgaagcgca cgagctcgtg ctcgcaagag aagtcagcgc gcaagcgcag
     5041 catccaccgc ttccaggaca ctgggaacct cctcgacaga gttcgtaaac tgcggagcaa
     5101 agcggagatt gccgtccggc atgctggcaa ccacgccatg cgcccgaagg ctcgacacga
     5161 gctctgcggc agtcttacct tcaggcggaa ccaaacagag catgcctgat cgagccccta
     5221 cggatgttga acgtaggctc tcgaagccgc gatccatcaa tggactctcg agtcggtcga
     5281 ggtaggcgga aacgtgactg aagatcgcgg gaacacccag ctcgagaatt ggatccagcc
     5341 ccgcttccat ggccacagag ctgagcgcgc tctgtgaccc gccttcgacg aagtcgatgc
     5401 tgcgcttgag gggtcgatca taacgcagct gaccgggttc gaacaaaaat ctcacgccgt
     5461 cttcgtggct gagccagccc gccatgcgtg gaattaggag gcgctgcagc tccccgcgcg
     5521 cgtagaggaa cccagcccct tgcgcaccca tcaaccactt gtgcgccccg cctgccgcat
     5581 agtcgatacc actcgcccga acatccaccg gtacaacgcc gcaagcttga atcaaatcca
     5641 cgcacagctc cgctccgtgc gcgtgacaca gctccgcaac ggcccggaga ggagcgcggt
     5701 aacccgtctg aaatgccacc gcgctgatcg ccacgagccg cacgccacca ccgcgtaacg
     5761 tggtctcgag ctccgaaaga tccgcatcct gggaccggtc tcgaccgccg acggaggctc
     5821 ctagagcctg cagttcgcag agctcgaggt cgaagcacgc ggccgcgttc tgccaaggcg
     5881 tgaagtttgc cggaaactcc cctcggaaga cgaccacgcg atctcctggt ttccaatcga
     5941 tgcaaaacgc gagctgcagc agcccgtgcg tcgttcccgt cgtgagcccg atctcttccg
     6001 cgcgcgcgtt cagcagacgc gcaaccttgc cgcgcaatcg ctcgcgttgc tcgatggtgc
     6061 gtccaatagc cagctgcccc acgcgcgcgt aatcttcgat cgactcatgc agcgcgtctc
     6121 tcacggcgcg agtagccgga gaaatcgcgg cgtaagcgag atagtaggga tactcaagat
     6181 cggcaacgag cgatcgatca cccacggcgg tgcggtattg aggcattctg cgtagattag
     6241 tccaaaccaa gcccgttcac agcaagagta cctcccatgc gccagcaggg cccgatctcg
     6301 atcgggcccc cattcgatca cttcgcctag attggggaga gtggcttcag cagtgaatac
     6361 agggtgctgg tcggtccaac gcggggtgcc gcaggcgcgg ataagaacgg ttcctcgtgc
     6421 tggttcccgc agcaacgatc ggattcgcgg ctacgggttt cgggagcacc gcaccgctgg
     6481 cgagttcttc gggccgcaga acagattcga gctgggcggg atcgaaccag cccggaagcg
     6541 ggcgccagcc aaagcgagcg cgatctcgct cggagttgcc ggccccttcc ctcgagctgg
     6601 cgccggaagc tgcggggccg gcgccgggat ctttgacgtc caacagggca atcgccgcgc
     6661 gcgtgccttg cccgccgagc tgcgtgtagc gcagagtcgt gctcatgctt cgatgcccga
     6721 gtaaggcttg gaccgaagcg aggggcgcgc cgcgcagggt gaggtgggct gcgaaagtcc
     6781 ggcgcagcac gtcccacccc aactgccgca aacccgcgcg cctcgccgcc cgatagagcg
     6841 gccacttgca ggctccgtga gtcagcattt caccctcctc cgtgcagaac acccacttgc
     6901 cacggcgggg gtgctgacga agttctgcgg cggtgtcttc actgagcgga atctcgcgcg
     6961 atcttacgtt ttcatccaga cgatctcggg agcgttgctg gacgcgcagt cgcaagtgct
     7021 cgaggtctac gttgtcccac gtgagtgcgc ggagctcacc caaacgaagg cctgtgcgca
     7081 agccgaggat gatcatcaag cgccaatcgg ggtcggccgc tcgcacgaga gcgcgagctt
     7141 cgtcgaaacc gagaaactgc aagtctggcg cttcgagcgc aaagctctga atttgaggcg
     7201 cggcaccaat gatctcgcgc gctatagcca gcgagaagac cgctcgcaat accactagat
     7261 gattgttgat cgtcttgcgg ctgaacccac tctccagctg ggcctccttg taagcctgca
     7321 cagcgttggt atcgaccgtg taaatgggtg acgcgccgaa cgccggaatc
//
LOCUS       DASTKU010000438         3542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1644018, whole genome shotgun sequence.
ACCESSION   DASTKU010000438 DASTKU010000000
VERSION     DASTKU010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644018"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1412
                     /locus_tag="VFQ61_15000"
     CDS             <1..1412
                     /locus_tag="VFQ61_15000"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9955816.1"
                     /translation="PTTDVIANPYTLKFHVDADAQVIRDLDAGLLADYADEVADWAVQ
                     GNKLNAFASCQSVDDNCGGQLISSLGLKLMREDLPASSERAKPYLALFKAEASFKDAA
                     AAVLSAMLQSPFFLYRREIGTADAGAFKLTPYEIASQLSYFLTDSPPDSQLLDAAKNG
                     QLSTQQQIDTQAARLLQTPQAKVTLRQFVEGWLDIDVLRVKTKDNKVVDLTPQMREAM
                     LAETTETFLDAFFNGGDLGKLLTSTDTFVNQDLGKFYGVNGANSTTPSKVSLAGSNRP
                     PGILGHAAFLTAHAQPENSSPLQRGLTIRGRLLCESIPPVPQGLNTALAAPGATKTNR
                     ERFEVHSKQEPCATCHKVFDPVGFAFENYDGFGRYRSNENGVNINATGVLAGMPEGDV
                     ALNGPQPLVDYLSKSDKVRACLVRYWSYYAHGRDNWTEKKCNDDSVRREAAANGNTLK
                     SVLMGILHAPSFTRRVNSQ"
     gene            1439..2773
                     /locus_tag="VFQ61_15005"
     CDS             1439..2773
                     /locus_tag="VFQ61_15005"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9955817.1"
                     /translation="MSILSRRDFARNAALAALFSPFINVLQPKTAQAAPGKAKFFLLF
                     FTNGRDTAAWRNSGSDSSLTLSAQTEPLSPIKSNLVIVEKCNSNGMAASHGAPGGLCG
                     GDWSSPLFSIEQFVSDGLKAAGVTRPVANIVLGGAEGNQPTNFYRDSKPLTPIYSPAT
                     AFQALFGNGVPTTTGGGGTTTPTTDPTAAARLKRKQSIISLVRGELTGLEKALGSVEK
                     QKLEAHLDSLAQLEKRIQGSMTSTGGQQQGGSTGGTQVSCKAPAKPSAASQPFLNSSI
                     HLDLAINAFACDITRVAAVQFGHHQMCPVDVEGAKGDYHNDFMHSDPAPHSRLANCEK
                     WLAGQFLAAVNKLKSLPAPDGSGTLFDQTIIMWARDMGDAPNHSGDDMAYIFSGGAGG
                     SLKTGGSGRFINANGASHQSALLTAASAMGLTNLSSFGSKTLSHEPLSGVLA"
     gene            complement(2897..>3542)
                     /locus_tag="VFQ61_15010"
     CDS             complement(2897..>3542)
                     /locus_tag="VFQ61_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9955818.1"
                     /translation="PQPGFLQRLLELCRMHGALSILDEVMTGCRLAPGGAQELYGLKP
                     DLTCLGKVVGGGMPLAVYGGRRDVMSQVSPLGPVYQAGTLSGNPLSVSAGLATLEQLV
                     PEKYAKLEATSARLANGLSEALEEIGIPGVVQRVGSMLTLFFNEGPVRNWNQAKSSDT
                     RRFGAFHRALTQGGVYWPPSQFEAAFVSFSHSHEDIDLTLHAVKAALGEARSVS"
BASE COUNT          670 a         1133 c         1108 g          631 t
ORIGIN      
        1 tccccacgac ggacgtgatt gcgaacccgt acacgctgaa gttccacgtg gacgcggacg
       61 ctcaggtcat ccgtgatctc gacgcggggc tgctcgcgga ctatgcggat gaggttgccg
      121 attgggccgt tcagggcaac aagctgaatg ctttcgcttc gtgccagagt gttgacgaca
      181 actgcggcgg ccagctgatt agctcgctag gcctcaagtt gatgcgcgag gaccttcctg
      241 cctcgagcga gcgggcaaag ccttacctgg ccttgttcaa ggccgaagcc agtttcaagg
      301 acgcggcggc cgcggtgctt tcggccatgc tccaatcgcc cttcttcctg tatcggcgtg
      361 agatcggtac ggcggacgcg ggtgcgttca agctgacgcc atacgagatc gcaagccagc
      421 tctcgtactt cctgacggat tcgccgccgg actcccagct gctcgatgcg gcgaagaacg
      481 gccagctgtc cacccagcag cagatcgaca ctcaggctgc tcgcctgttg cagacgcccc
      541 aggcgaaggt gaccttgcgt cagttcgtcg aaggttggct cgatatcgac gtgctgcgcg
      601 tcaagacgaa agacaacaag gtggtcgacc tcaccccgca gatgcgtgag gcaatgctgg
      661 cggagacgac ggagaccttc ctcgacgcct tcttcaacgg cggggatctc ggcaagttgc
      721 tcaccagcac ggacaccttc gtcaaccagg acctcggcaa gttttacggc gtgaacgggg
      781 ccaactccac cacgccgagc aaggtgtcgc tcgcgggtag caatcgcccg ccgggcattc
      841 ttggccacgc cgcgttcctc accgcccacg cccaaccgga gaactcttcg ccgctgcaac
      901 gtggactcac gatccgaggc cgcttgctgt gtgagtcgat cccgccggtt ccacagggcc
      961 tcaacacggc tctggccgcg cccggcgcga ccaagaccaa tcgcgagcgc ttcgaggtgc
     1021 actcgaagca agagccgtgc gccacttgcc acaaggtctt cgatccagtc ggcttcgcct
     1081 tcgagaacta cgacgggttc ggccgctatc ggtccaacga gaacggcgtg aacatcaacg
     1141 ctacgggtgt gctcgccggc atgccggagg gcgacgtcgc gctcaacggc ccgcaacctc
     1201 tggtggacta cctgtccaag agcgacaaag tacgagcgtg cttggttcgc tactggtctt
     1261 actacgcgca cggccgcgac aactggacgg aaaagaagtg caacgatgat tcggttcgtc
     1321 gtgaagcggc tgcgaacggg aacactttga agagtgtgtt gatgggcatc ttgcacgccc
     1381 cgagtttcac gcgccgagtg aactctcagt gagtcgagaa tagggagcaa cacagcaaat
     1441 gagcatttta tctcgccgcg acttcgctcg aaacgccgct ctagccgcgc tattctcgcc
     1501 tttcatcaat gtgctgcagc cgaagaccgc gcaggcggcg ccgggtaaag ccaagttctt
     1561 cctgctgttc ttcacgaacg gcagggacac ggcggcctgg cgcaacagcg gctcggactc
     1621 gagcctcacg ctcagcgcgc agacggagcc gctctcgccc atcaagagca acttggtgat
     1681 cgtcgagaag tgcaacagca acggcatggc tgcgagccac ggcgcgcccg gtgggttgtg
     1741 cggtggtgac tggagcagcc cgttgttctc catcgagcag ttcgtctcgg acggcttgaa
     1801 ggcagccggc gtcacgcgcc cggtggccaa catcgtgctc ggtggcgccg aaggcaacca
     1861 gccgacgaac ttctatcgag acagcaagcc gctgacgccg atctactcgc cggctaccgc
     1921 cttccaggcg ctcttcggta acggcgtccc gaccaccacc gggggcggcg gtactacgac
     1981 cccgacgacg gatccgacgg cggcagcccg cctcaagcgc aagcagagca tcatcagctt
     2041 ggttcgtggt gagctcacgg gcctcgagaa agcgctcggc tcggtcgaga agcagaagct
     2101 cgaggctcac ttggattcgc tggctcagct cgagaagcgc atccagggct cgatgacgtc
     2161 gacgggtggt cagcaacaag gtggcagcac gggcggtact caggtgtcgt gcaaggcgcc
     2221 cgccaagccg agcgccgcgt ctcagccgtt cctcaacagc tccattcacc tggatctcgc
     2281 gatcaacgcc tttgcgtgtg acatcacgcg cgtggccgcg gtgcagttcg ggcaccacca
     2341 gatgtgtccg gtggacgtcg agggcgccaa gggcgactac cacaacgatt tcatgcactc
     2401 cgatccggcg ccgcactcac gcctggcgaa ctgtgagaag tggttggcag gtcagttcct
     2461 cgcggcggtg aacaagctga agtcgcttcc ggcgccggat ggcagcggca ccttgttcga
     2521 tcaaaccatc atcatgtggg cccgcgacat gggcgacgcc ccgaaccact cgggcgacga
     2581 catggcgtac atcttctcgg gtggcgcggg tggctcgctg aagaccggtg gcagcgggcg
     2641 gttcatcaac gccaacggcg cctcccacca gagcgcgctg ctcaccgccg cctccgcgat
     2701 gggcctcacc aacctatcca gcttcggttc caagaccttg agtcacgagc ccctttcggg
     2761 cgtgctggcc taagttctgc ggttgcgaag caatgagagg gatccaggaa actggatccc
     2821 tcttctgcat ttgagggcat cgggttctag gcgtagcccg atcgcgaagt cgactaggct
     2881 ggttgcgggg agcggttcag ctcacgctac gtgcttcgcc gagcgcggct ttgaccgcat
     2941 gcaacgtaag atcgatgtct tcgtggctgt gggagaaaga cacgaacgcg gcctcgaact
     3001 gtgacggagg ccagtacact ccgccttggg tgagcgcgcg atggaaggcg ccgaagcgac
     3061 gggtatcgct gctcttggct tggttccaat tgcgaaccgg gccctcgttg aagaacagcg
     3121 tcaacatgga gccgacccgc tgcaccacac ccgggatgcc gatttcttct agggcctcgc
     3181 tcagaccatt ggcgaggcga gcgctagtgg cctcgagctt cgcgtatttc tccggcacga
     3241 gctgttcgag cgtggccagc ccggcgctca cgctgagcgg gttaccgctc aaggtgcccg
     3301 cttgatagac gggcccgagc ggtgacactt ggctcatcac gtcgcgtcgg ccgccgtaga
     3361 ccgcgagcgg catgccaccg cccactactt tacccaagca agttagatcg ggtttcaacc
     3421 cgtagagctc ttgggcaccg cccggagcca gtcggcagcc ggtcatgacc tcgtccaaga
     3481 tgctgagcgc gccatgcatc ctgcacagct cgagcagccg ctgcaggaag ccgggctgcg
     3541 gt
//
LOCUS       DASTKU010000439         6155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_751443, whole genome shotgun sequence.
ACCESSION   DASTKU010000439 DASTKU010000000
VERSION     DASTKU010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751443"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VFQ61_15015"
     CDS             complement(<1..753)
                     /locus_tag="VFQ61_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955819.1"
                     /translation="MMFRSLRTSCTTLPVLLLIACSAPIDIGDKDSPLGEVGGLANTG
                     AAGGGGGGTLRPQGGSGGSTDPWTGGLSGTGGTQDPGVGGSGGSGTAGGTEFGSGGLF
                     WGTAGSTEPGSGGVWGTAGGTEFGSGGLFWGTAGSSEPGSGGVWGTAGSSEPGSGGVW
                     GTAGGTYIPGLGGSGPWGGSAGSSEPGVGGSSGWSGSTEPGSGGVSSGGVWGTAGGTY
                     IPGLGGSGASGGWSGVGGASTLPGSGGVSACGM"
     gene            1079..2203
                     /gene="rsgA"
                     /locus_tag="VFQ61_15020"
     CDS             1079..2203
                     /gene="rsgA"
                     /locus_tag="VFQ61_15020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET9955820.1"
                     /translation="MSHVSMDECPSLLRELGLTEEWLAQVEQLAAEERGWGRWPLRVV
                     ADRGAELGLLGMNGPQRAVLSGRLRHEPGELQAPCVGDWVMVSEHAGMLRVEHVLARH
                     GLFARKSVGGSSAVQPIAANVDLAVIVAAFAPSESDPYAAQHALNVRRIERYQLAAAQ
                     AGARALLALNKADLCQDSIERAARLADERGLQVLATSTRAELGIAELAQAIAQAGTAV
                     LVGSSGVGKSSLTNALLGAAAQATGEVRDEDSKGRHITSHRELFMLPSGGCLIDTPGM
                     REFAPVGDAPQDSEFASELERLGQTCKFRDCQHAGEPGCAVQQAIASGALDQDRLAHH
                     LQLMREVASQRDRHDARARAQTKKRGKTISKAVRDFYKRR"
     regulatory      2280..2482
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2491..3324
                     /locus_tag="VFQ61_15025"
     CDS             2491..3324
                     /locus_tag="VFQ61_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HET9955821.1"
                     /translation="MRSTPGRSRADLTGSALPSKLPVVIIAAVISSILLDAAPAHAMH
                     LSDGVLPGSWCVVWTLVALSFVGLAVRRFELRQARDARLMPFLAMVGAGVFAISCMPI
                     PVPWLGTCSHPCGTGLAALLVGPLFTVLLTAVALLLQALFLAHGGLTTLGADIVSMGV
                     AGGFAGYLSYRIARRAGVGEFAAAFLAGILADFATYATTALAIALGLAGAGVKSGGAG
                     PVQSVGELFATVCLAFAPTQLPLGVLEGAVTAFAFVFVRRRRPAMLGELQVLLGARRQ
                     P"
     gene            3321..3677
                     /locus_tag="VFQ61_15030"
     CDS             3321..3677
                     /locus_tag="VFQ61_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955822.1"
                     /translation="MRRAEFVLSARGRSEARASRWRDAVLVVAVAGLALLGFFRLLAK
                     QAPHSGDWPGVDETVVEHFARQAGAPQLGFRIAWVQGDLLLFAFLCAGLAAGFVLGYC
                     GRTLFGEAERGRRDAE"
     gene            3667..4350
                     /locus_tag="VFQ61_15035"
     CDS             3667..4350
                     /locus_tag="VFQ61_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HET9955823.1"
                     /translation="MPSDAQSARIPELSPAWRLAGWIAGLFGIVLWPRPVEVAIVGGI
                     CVLGLLISSGGRRIIRRRLGVALPWIVLALGAWALQERHLPISSSQRLMLALRVTVGS
                     LFSGAMARGLRSGELRRGLQELRLPRSFVELLLETRALGRSLTETLTSALCASTLRGS
                     GASLRTRARAAGTVAGVVLVRCLDRADRRVTALALRGYGMARVSGNANAEPSVDTTAG
                     NAIEQMRRA"
     gene            4347..5162
                     /locus_tag="VFQ61_15040"
     CDS             4347..5162
                     /locus_tag="VFQ61_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9955824.1"
                     /translation="MTALRVDAPFIGYAGAAPALRDVRFEMEYGECIALLGGNGAGKT
                     AMLRYIAGLLPRAGGRCTLEGREIRSARDAVALGVGLVVQEPDDQLLGDTVRDDAEFG
                     PHNLGLSNSECAQRVDQALAWVGLLELATREIESLSLGERKRASLAGVLAMRPRVLLL
                     DEPTAGLDPQGERAICEILNQLRRAGCALLVATHAVDLIPYFASRVLVLGAGTIRADG
                     ACREILLNAELLEASRVRRPWALELWARLLAPHVPTPTLSLEEVLSCWTTESF"
     gene            5138..>6155
                     /locus_tag="VFQ61_15045"
     CDS             5138..>6155
                     /locus_tag="VFQ61_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HET9955825.1"
                     /translation="MLDDGVLLIGHGSREPGANAEFEALAADYRARHPEWCVETAYIE
                     LAAPLVQDALTQLAGRVRRLTVVPVFLFAAGHVKNDLPLALERARLAFPGTRFVVSPA
                     LGVHPRLAELAFRRVDEAMQSCGARPESTLLLVVGRGASDPDANGDFYKMARLIAEGR
                     GLLHVEAAFMGITQPSVPRALEWIARLRPPRLLVLPYLLFGGRLVTKLAQQIDEFCAR
                     HPWIVGSMASYLGQHELLLDLIEERVSQARAGAVLLPCDNCQYRVGLPGRASEVGGLR
                     ALLYSVRHSLTHSDVQAPEHRHRPLRKHVLVCGNADCVDRGSIVLLETLRRELKQLGR
                     ARDIL"
BASE COUNT          924 a         1965 c         2069 g         1197 t
ORIGIN      
        1 cattccacat gcgctcacgc ctcctgaacc gggcagcgtg ctcgctccgc caacaccgct
       61 ccagccgccc gaggcgcccg acccgcccag gcccggaatg taagttcctc ccgcggtgcc
      121 ccagacgcca ccactgctca cgccaccact accgggttcg gtgctgccac tccagccact
      181 cgagccgccg acacccggct ccgagcttcc tgcggagccg ccccatggtc ccgatccgcc
      241 caggcccgga atgtaagttc cacccgcggt gccccaaacg ccaccactgc cgggctcgga
      301 gcttcctgcg gtgccccaaa cgccaccact gccgggctcg gagcttcctg cggtgcccca
      361 aaacaaccca ccacttccaa actcggtgcc acccgcggtg ccccagacgc caccactgcc
      421 gggctcggtg cttcctgcgg tgccccaaaa caacccacca cttccaaact cggtgccacc
      481 cgcggtgccc gacccgccag agcctccgac gcccgggtcc tgagtgccgc cggtgcccga
      541 gagcccacct gtccacggat cggtactgcc gcccgagcct ccttggggcc tcagcgttcc
      601 gccgcctccg ccgccagcgg cgcctgtgtt cgcgagcccc ccaacttcgc ccaaggggct
      661 gtctttgtcg ccgatgtcga tgggagccga gcaagcgatc agcaacagga ccggcagcgt
      721 ggtgcaactc gtacgaagcg aacgaaacat catcaacaac ctccgtgccc tatcgaagct
      781 ccaagtgtac ggcttggatc gacccttgtt cgattttgtt tggttacctg ctcggggccc
      841 cggctcgagg ggacgcaccc gcgatgctct cgggattctt tcactcgctc ggacggatgg
      901 tatttgccgg ctgagagccc gtctccatag gagttcgcct ccgctgacca attgctccct
      961 ctcggccact ggcctcgggc gctcctactg gggtgggtcc ttaccttgac gagtggctca
     1021 actcagcaca cctttcgccc gaccggatga gagccagctt gttctgcctg taggtggtat
     1081 gtcgcacgtt tcgatggatg aatgcccaag cttgctgcgt gagttggggc tgaccgaaga
     1141 gtggcttgcg caagtcgagc agctcgcggc tgaggagaga ggctgggggc gatggccgct
     1201 gcgcgtggtc gcggatcgag gcgccgagct cggcttgttg gggatgaatg gtccgcagcg
     1261 cgcggtgctg agcgggcgac tgcgccatga acccggtgag ttgcaagcgc cttgtgttgg
     1321 cgattgggtc atggtgagcg aacacgccgg aatgctgcgc gtcgagcacg tgctcgcgcg
     1381 acacgggctg ttcgcgcgca agagcgtagg cgggtcgtcc gcggtgcagc cgatcgccgc
     1441 caatgtggac ttggcggtga ttgtagcggc ctttgcgccg agcgagagcg acccgtatgc
     1501 agcccagcac gcgctcaacg tgcgtcgtat cgagcgatac cagctggcgg ccgcgcaggc
     1561 gggcgctcgg gccctgctgg ccttgaataa ggcggacttg tgtcaggatt cgatcgagcg
     1621 cgcggcgcgg ctcgcggacg agcgtgggct gcaagtcttg gcgacgagca cgcgcgctga
     1681 gcttgggatc gccgagctcg cgcaagctat cgcccaggct gggaccgccg tactcgtcgg
     1741 ttcttcgggc gtcggtaaat cgagtctcac caacgcgttg ctcggcgcgg ccgcgcaggc
     1801 cactggggaa gtgcgcgacg aggactcgaa gggccggcac atcacgtcgc accgtgagct
     1861 attcatgttg ccctcgggcg gctgtttgat tgatacaccc ggcatgcggg agtttgctcc
     1921 cgtaggagac gctccccaag actcggagtt tgcgtccgag ttggagcgtt tggggcaaac
     1981 ttgcaagttt cgagattgcc aacatgcggg tgagcccggc tgcgccgtgc agcaagcgat
     2041 cgcgagcggc gccctcgatc aggatcggct cgctcatcac ctgcagttga tgcgtgaagt
     2101 ggccagtcaa cgtgaccgtc acgacgcgcg cgcccgcgca cagacgaaga agcgaggaaa
     2161 gacaatctcc aaagccgtgc gagatttcta caagcgacgc tagagcagcg ctcatgcgct
     2221 gctctagcca ttcgtcccct tgactcagaa gcgggagcgt tttaagcagc ggccctgaca
     2281 atttgaaatg agcttcgggt gcccgcgagg gcacaatagg gaagccggtg caagaccggc
     2341 gcggtcccgc cactgtaagt gggaagccga ttcacgagcc actggatccc catcggggcg
     2401 atctgggaag gcgaactcgg cgctgcagca gtcgctgctg aacccacgag ccaggagacc
     2461 tgcccgaggc ataacctcgc gaggtgagcc atgcgctcca ctccggggcg ctcccgtgcg
     2521 gatctcaccg gcagcgccct tccttcgaag ctacccgtcg tcatcattgc tgctgtgatc
     2581 tcgagcatcc ttttggatgc tgcgccggcg catgcaatgc atctctccga tggggtgtta
     2641 cccgggtcct ggtgtgtggt ttggacgctg gtggctttgt ccttcgtggg gctcgccgtg
     2701 cgccgcttcg agctgcgcca ggcgcgtgat gcgcggctga tgccgttcct cgctatggtg
     2761 ggagcgggag tattcgcgat ctcctgcatg ccgatcccgg taccttggct cgggacttgt
     2821 tcccacccct gcggtaccgg ccttgcggcg ttgctcgtcg gcccgctctt cacggtctta
     2881 ctcaccgcgg tcgccttact gctgcaggcc ttgttcttgg cgcatggcgg gctcaccacg
     2941 ttgggggccg atatcgtgtc catgggggtg gcgggtggct ttgcagggta cctgagctat
     3001 cgaatcgctc ggcgcgcggg agtcggtgag tttgcggccg cgttcttggc tgggatcttg
     3061 gcggattttg cgacctacgc gacgacggca ttggccatcg cgctcggctt ggcgggcgct
     3121 ggtgtgaagt cgggcggcgc cggtccggtt cagtcggtgg gcgagctgtt cgcgacggtg
     3181 tgcttggcgt tcgcaccgac gcagttgcca ctcggagttt tagagggcgc cgtcacggcc
     3241 ttcgcgttcg tcttcgtgcg gcgtcggcgg cccgcgatgc tgggtgaact gcaagtcttg
     3301 ctcggtgcgc ggaggcaacc atgaggcgcg cagagttcgt tttgtcagct cgagggcgga
     3361 gtgaggctcg agcctcgcgt tggcgtgacg ccgtactggt ggtcgcggtg gccggcttgg
     3421 cgttactggg tttcttccgc ttgctcgcca agcaagcgcc ccattcagga gactggccgg
     3481 gggtggacga gacggtggtc gagcacttcg ctcgccaagc cggcgcgccg caattgggct
     3541 tccgcatcgc ctgggtccaa ggcgacttac ttctattcgc cttcctgtgt gcggggctcg
     3601 cggccggatt cgtacttggc tactgcggga gaacgctgtt tggagaggcg gagcgaggtc
     3661 gacgtgatgc cgagtgacgc tcagtctgct cgcatcccgg agctctcgcc cgcatggcgc
     3721 ctggcggggt ggattgcggg cttgtttggt atcgtgcttt ggccccgccc ggtcgaagtc
     3781 gcgatcgtgg gtgggatttg tgttttgggg ttgctgatat cgagtggcgg tcgaaggatc
     3841 atccggcgac gactgggcgt ggctctgcct tggattgtct tggcgctcgg cgcttgggcg
     3901 ctgcaagagc ggcacctccc gatctcctcg tcccagcgcc tgatgctcgc actccgggtc
     3961 acggtgggct ccttgttctc gggtgcgatg gcgagggggt tgcgttcggg cgagctccgg
     4021 cgaggccttc aggagctccg gttgccgcgc tctttcgtcg agttgctgct cgaaacgaga
     4081 gcgctagggc ggagcctgac cgaaacgctg accagcgctc tttgtgccag caccctgcga
     4141 ggttctggag cttcgcttcg aacccgtgcg cgcgcggcgg gcacggtggc aggcgtagtg
     4201 ctcgtccgct gcctcgatcg cgcggatcga cgcgtcacgg cgctggcctt gcgcggctac
     4261 ggcatggctc gagtctcggg caacgcgaat gccgagccga gtgtcgacac gaccgctggc
     4321 aacgccatcg agcaaatgag gcgcgcgtga ccgcgttgcg cgtcgacgct ccgttcatcg
     4381 gctacgccgg tgccgcgccc gcgttgagag acgtccggtt cgaaatggag tacggcgaat
     4441 gcatcgcctt gctcggtggc aacggtgccg gcaagacggc gatgctgcgc tacattgccg
     4501 gtttgctacc gcgcgccggg gggcgctgca cgttggaggg ccgcgagatc cgatccgcac
     4561 gcgatgcggt tgctctcggc gttggtttgg tggtccaaga gcccgacgac cagctgctcg
     4621 gagacacggt tcgggatgat gccgagttcg ggcctcacaa cttgggactc agcaattcgg
     4681 agtgcgcgca gcgcgtcgac caagctctag catgggtcgg tttattggaa ctggcgacgc
     4741 gcgagatcga atccttgagt ttgggagaac gcaagcgagc ctccctggcg ggcgtgctcg
     4801 cgatgcggcc ccgcgtgttg ctgctcgacg aacccacggc aggtctggac cctcagggtg
     4861 agcgcgcgat ctgtgagatc ttgaaccaat tgcggcgcgc cgggtgcgcg ctgctggtgg
     4921 ccacccacgc tgtggatttg attccatact tcgcctcgcg cgtgctcgta ctcggagccg
     4981 gcaccattcg cgccgacggg gcttgccgag agattttgtt gaatgcagag ctcctcgaag
     5041 cctcgcgcgt gcgacggcct tgggccctcg aactgtgggc ccgcctgtta gcgccgcatg
     5101 tgcccacgcc caccctgagc ttggaggagg tcctgtcatg ttggacgacg gagtccttct
     5161 gatcggtcac ggcagccggg aaccgggcgc gaacgccgag ttcgaggcgc tagccgcgga
     5221 ctatcgcgcg cgccaccccg agtggtgtgt tgaaacagcc tatatcgaat tggctgcgcc
     5281 tctggttcaa gacgcattga ctcagcttgc aggccgcgtg cgccgcttga ccgtggtccc
     5341 cgtcttccta tttgcggcgg ggcacgtaaa gaacgatctg ccgctggctt tggagcgagc
     5401 gcgcctcgcg tttcccggca ctcggttcgt cgtttcaccc gcgttggggg tgcatccgcg
     5461 cttagccgag ctcgcgtttc ggcgcgtcga cgaggcaatg caaagttgtg gcgcgcgccc
     5521 cgaatccacc ttgctgctcg tggtgggacg aggggccagc gatcccgacg ccaacggcga
     5581 cttctacaaa atggctcgcc tgatcgcgga ggggcgcggt ctcttgcacg tcgaggccgc
     5641 gttcatgggc atcacgcaac ccagcgtgcc gcgagccctg gagtggatcg cgcgcctacg
     5701 gcccccgcgc ctgctcgtcc taccctatct tttgttcggc ggacgcctgg tcaccaagct
     5761 cgctcagcaa atcgacgagt tctgtgcccg tcacccttgg attgtgggca gtatggcgtc
     5821 gtatctagga caacacgagc tcctactcga cctgatcgag gaacgagttt cccaagctcg
     5881 agcgggagcc gtcctcttgc cgtgcgacaa ctgtcaatat cgcgtggggc tgcccggccg
     5941 cgccagcgag gtgggaggct tgcgagcgct gctctacagc gtgcggcatt cgctgaccca
     6001 ctcggacgtg caggcgccgg agcatcgtca ccgtccgctc cgcaaacacg tgctcgtctg
     6061 cgggaacgca gattgcgtcg atcgaggcag catcgtgctg ctggagacgc tccggcgcga
     6121 gctcaaacag ctcggtcgag cacgcgacat tctgg
//
LOCUS       DASTKU010000440         6927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_305948, whole genome shotgun sequence.
ACCESSION   DASTKU010000440 DASTKU010000000
VERSION     DASTKU010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305948"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VFQ61_15050"
     CDS             complement(<1..1472)
                     /locus_tag="VFQ61_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955826.1"
                     /translation="MRRIRTALFFGFGLCAAAGCELGRDVVAVPMRSTTPQGGGGASS
                     GAPTTYPSYAAQFGTGYGGATSTSTNNGSGQGGLLEGSGGYPYEPYAGAPSAGDGNAG
                     SAGATGMPECSPAHPEVQVMSGSRMRTICTGTLARDRMSHAVCTCGDLAVQGLLATSA
                     YDSLEASSLNTTGAAVGVNGTYTSGDYARVQGSFTIAGTSLLTLVGLDVAGDLKLAAT
                     GVSLGPIQVGRDAWFARSVSVLDETRIARDLHLARNATVTGLRDERVGGRTVRDVTEI
                     APPCRCGSEATFDLAANIETAREQNDNDRIGLSVQKLNQAASVAGAAGEGGSANTFEL
                     PCGTYFLSDMPISQPLQLRVTGRVALFVAGDVALRNDFTLDLGAEAELDWFIAGRLDL
                     RGATRVGDPERAARLRIYVGSSSEITFPSAHVAGNLYAPRADVTVTGVGDVYGSIFAR
                     NLTSADALTVHYDRAILNLGDQCPAPANTACAGCGECGAGL"
     gene            complement(1472..2983)
                     /locus_tag="VFQ61_15055"
     CDS             complement(1472..2983)
                     /locus_tag="VFQ61_15055"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9955827.1"
                     /translation="MTDACERWMEVSDRLMVGEQISAHEREFLRVHASTCSECSVEAA
                     ALRELKPLPLHSVPSHEEVDRILQAAFASERSVQVAEHPVLDDSQLETTLLYVPKRAE
                     PAPGFAPEPERDPRSLRERIPERISQPLPQRPAAPRFRAWTAGLLTACGVAAAAAGFL
                     VFRQLPRAAIDTASAAVGPSPAPSVGAAQGSPRVALSDSGAGSHEVITRNQEDTCGEV
                     LSGVTLCLTRGSEVSRVELAGADRTVYLKRGRAIASLAPQPPGTSFSIVTASGRVTAV
                     GTVFSVEVTAEGGSIARVARGKVLVRAPAAGADHALLAGQMLRLGESESLRLTRTDQD
                     QDLSLLEQLAPDSPGLVAHGAIIDLDANDEESPPRRVSRKVKDQLQRARELRKRGDFS
                     GAAAVYRAVHQDNPHSAEGRAALLSLAGLLLSPLNDPASALSAYNAYLATGRGTLAAQ
                     AEYGRIRALRSLGRTSEEHSATEAFLEHFPNAPEARALKQPKPSSSSRRELMR"
     gene            complement(2980..3600)
                     /locus_tag="VFQ61_15060"
     CDS             complement(2980..3600)
                     /locus_tag="VFQ61_15060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9955828.1"
                     /translation="MTASKRSTVEAADSAGDERPVRATNGAPDVLLMQAAAAGDPAAQ
                     RAVVVRLMRRIQRLCKALLRHREDAGDASQASLLAILRAAGGFRGESSLERWADRIAV
                     RTALRQSRERRREALQPVDENALPAVHSSGDPAIAAGQYLDLLPERQRSVLILRCGFE
                     YSVEEIAELMQTSPNTVKDRLKRARAGVRRALGREETLRLAAGQRR"
     gene            complement(3691..4800)
                     /locus_tag="VFQ61_15065"
     CDS             complement(3691..4800)
                     /locus_tag="VFQ61_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955829.1"
                     /translation="MNRIHFSLLAALVFSSLATAPCLAAGNPEATQAADVPRVVLVFD
                     PTRERALAAVQAIESHLSGLRVSVDVVGISLAAGMNEWLDQASQQAASREALGSFAID
                     ATPRGELLIFFTEVSGEATLLRRIRPKTAGGRVALEEAGIVVSSLVEALLDGRHIGME
                     PSAPSPTSDDHTLRDSERTPVGEVAPPPRASAPSQSVRHTAVSLGYLGTSFAQDTFQS
                     GLVLGARTDLAERWSLLASYAILPELELDREGVHLSLQRHPLVLGLGYEAAGTLAPLV
                     EVTGFSDWVTRRTPSTQATLAGTPDQNHWMWGIGSRIGAHWGPASRLHVHVTFGVDVP
                     IGNLSFVVEGESPRTVIVPRSVRPQAEASLIANLW"
     gene            complement(4956..6236)
                     /locus_tag="VFQ61_15070"
     CDS             complement(4956..6236)
                     /locus_tag="VFQ61_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9955830.1"
                     /translation="MWAAPRQLVFLPEVSPMHIGVALSRGALSFSSLSALLLLSSCSV
                     TANEDEGTSGPGAGTSPRLPTPSGNSGGAIGAPNPGGVGGFSSGSATSLPSSPSCMPQ
                     PDDAGCVGEQYAGETVPLDVYVLFDQSCSMSCPISLGGPGRCCMGDPNARILPVRQAM
                     AAFLRDPRSAGIGLGLGYFGQRPLGQASCRPTDYETPDLPIGDAQAEALIASLEVAQP
                     TGETPTGAALRGACTYIDRARSERPGRSVALLLVTDGIPETPLTNCGATLTDAVAAAR
                     ECSSRPVPIKTYVLGIGQALSNLDQIAAAGGTQEAYLVEGGDVARMMLEALNAIRGKA
                     SIPCQLEIPPAPSGRDLDTQEVNLGLCDADGNAQSTLYVGSSSDCSSTGGWYYDDPAN
                     PRQILLCPTSCAIASTPGAQLYYSVGCQTQVPIR"
     gene            complement(6244..>6927)
                     /locus_tag="VFQ61_15075"
     CDS             complement(6244..>6927)
                     /locus_tag="VFQ61_15075"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9955831.1"
                     /translation="IDREVFARAQAGFPWTAYEDCPHIAQVGEETQVIGQGWVACTAI
                     VGPGGPLGIFFNDTAISHHPLDESRQWRAAILCSILANALWRLKTTDVSPTEQRETPQ
                     PEIVRRVARLLTQDPTLSCDALARQLNLSPRGLARSFKKEAKSSIVDYRNELRLASFL
                     ERAGAHPGNLLQAALEAGFGSYAQFHRVFRARFGRAPSDYLLEKAPPSSRSTSGSEVS
                     PPDEQAPGH"
BASE COUNT         1248 a         2296 c         2213 g         1170 t
ORIGIN      
        1 aggccagcgc cacactctcc acagcccgcg caagcggtat tcgcgggcgc gggacactgg
       61 tctcccagat tcaaaatcgc gcggtcgtag tgcacggtca gagcgtccgc ggaagtcaaa
      121 ttgcgcgcga aaatcgagcc ataaacgtcg ccgaccccgg tcacggttac gtcggctcgg
      181 ggcgcataca agttgccagc aacgtgcgcg ctcgggaacg tgatctcaga agagctgccg
      241 acgtagatcc gcaggcgcgc cgcgcgttcc ggatccccga cgcgggtcgc tccccggagg
      301 tcgaggcgcc cagcaatgaa ccagtcgagc tcggcttcgg cgccgaggtc cagcgtgaag
      361 tcattacgca gcgccacgtc acccgcgacg aacaacgcca ctcggcccgt cacgcgcagc
      421 tgcagcggct gcgagatcgg catatcactc aggaagtagg ttccacaggg cagctcgaag
      481 gtgttcgcgg agccgccttc tccggcggcg cccgcgacgc tcgcggcttg attcagcttc
      541 tgcaccgaga gcccaattcg atcgttgtcg ttttgctcgc gcgcggtctc gatgttggcg
      601 gccaaatcga aggtcgcctc ggagccgcaa cggcagggcg gcgcgatctc ggtcacgtct
      661 cgcacggtgc gcccgcccac gcgctcgtca cgcaatccgg ttacggttgc gttgcgcgcg
      721 aggtgcaagt cgcgggcaat tcgggtctcg tccaacaccg acacggagcg agcgaaccaa
      781 gcgtcgcggc ctacttgaat cggacccaga gaaacgccgg ttgcggcgag cttcagatca
      841 cctgctacat ccagtccaac cagcgtgagc aggctcgtgc cggcgatcgt gaagctgcct
      901 tgaacgcgtg cgtagtcgcc gctggtatat gtgccgttga cacctaccgc cgcgcccgtc
      961 gtgtttaggc tcgaggcttc gagcgaatca tacgcgctgg tcgcgagcag cccctgcacc
     1021 gcgaggtctc cgcaggtgca caccgcgtga ctcatgcgat cacgcgccaa agtacccgtg
     1081 caaatggtcc gcattcggct accactcatc acttggactt caggatgtgc ggggctacat
     1141 tcgggcatgc ccgtcgcgcc ggcgcttccc gcgttgccgt cgccggcgct cggtgcgccc
     1201 gcgtaaggtt cgtacggata gccgccgctg ccttcgagta gccctccttg accgctgccg
     1261 ttgttggtag aagtggaggt cgctccaccg tagccggtgc cgaactgcgc ggcgtagctc
     1321 gggtatgtgg tcggcgcgcc cgaactcgcg ccgccgcccc cttgcggcgt ggtggagcgc
     1381 atcggcacgg ccacgacatc ccgccccagc tcgcaaccgg cggccgcgca aagcccgaag
     1441 ccaaagaaca gcgccgtccg aatgcgtcgc atcaacgcat gagctcccga cgactcgacg
     1501 agctcggctt cggctgcttc aacgcgcggg cctcgggtgc gttcggaaaa tgctcgagga
     1561 acgcttctgt tgccgaatgt tcctcgctcg ttcgtcccaa gctgcgcagc gcgcggatcc
     1621 ggccatactc ggcctgggcc gccaacgtac cccgcccagt tgccaagtag gcattgtaag
     1681 cgctcagagc gcttgcggga tcgttgagtg gcgatagcag caggcccgcc agcgatagca
     1741 gcgccgcgcg cccttcagcg ctgtgagggt tatcctgatg aacggctcgg tagaccgccg
     1801 ccgcgcccga gaagtcgcct cgcttgcgca gttcgcgcgc gcgctgcaac tggtctttca
     1861 ccttgcgaga tacgcggcga ggcggcgact cctcgtcgtt cgcatccaga tcgatgatcg
     1921 ctccgtgcgc caccagcccc ggtgagtcgg gcgcgagctg ctccagaaga gatagatctt
     1981 ggtcctgatc ggtgcgcgtg agtcgcagtg actcgctctc tcccaagcgc agcatctgcc
     2041 cggccagaag tgcgtggtcg gcaccggcag cgggtgcgcg aaccaagacc tttccgcgcg
     2101 cgacgcgagc gatggaccca ccttccgccg tgacttcgac cgaaaatacc gtgcctaccg
     2161 cggtcactcg accggacgcg gtgacgatac tgaacgaggt gccaggcggc tgcggcgcga
     2221 gcgaggcgat ggcgcgtccg cgcttcaagt acaccgtgcg atcggcgcct gcgagctcca
     2281 cccggctcac ctcggagcct cgcgtgagac aaagcgttac cccactcaac acctcaccac
     2341 aggtgtcctc ctgattccga gtgattactt catgcgagcc ggctccgcta tcgctcagag
     2401 cgacgcgcgg gctaccctgc gcagccccga cgctaggagc aggggaaggc ccaacggccg
     2461 cggatgcagt gtcgatcgcg gctcggggaa gctgccggaa caccaggaag ccagccgccg
     2521 cagccgctac accgcaggcg gttagcagcc ccgcggtcca ggcgcgaaac cgaggcgcag
     2581 ccgggcgctg gggcaggggc tgcgaaatgc gctccggaat acgttctcga agagaccgcg
     2641 gatcgcgctc cggctccggc gcgaacccgg gagcaggttc ggcgcgtttc ggaacataga
     2701 gcagcgtggt ttcgagctgc gaatcatcca gtactggatg ttccgcgact tggacggatc
     2761 tctcggacgc gaacgcggcc tgcagaattc gatcgacctc ttcgtgactt ggtacggagt
     2821 gcaacggcag cggcttcaac tcgcgcaggg ccgcagcctc taccgaacat tcgctacacg
     2881 tcgaggcatg cacgcgtaga aactcacgct cgtgcgcgct gatctgctca ccgaccatca
     2941 agcggtcgga gacttccatc cagcgttcgc aggcgtctgt catcgtcgtt ggcccgcggc
     3001 aagccgcaga gtctcctcgc gcccaagcgc ccgccgcact ccggcgcgcg cgcgtttcag
     3061 tcggtctttg acggtgttcg gggaagtttg catcagctcc gcgatctcct caaccgagta
     3121 ttcgaagccg caacgcagaa tcagcacgct gcgttgacgc tcgggcagca gatcgaggta
     3181 ttggccggct gcgatcgccg gatcgccgct cgaatgaacg gcgggtaaag cattctcatc
     3241 tacgggttga agcgcttcac ggcgccgctc ccgcgactgg cgcaatgccg tgcgtacggc
     3301 gattcgatcg gcccaacgct ccaacgagct ttcgcctcta aacccacccg cggcgcgcaa
     3361 aatcgcgagc agactggctt ggctcgcgtc gcccgcgtct tctcgatgac gaagcagagc
     3421 cttgcagagc cgttgaatgc gtcgcatcaa ccgcaccacc accgcgcgct gtgcggcggg
     3481 atcccccgcg gctgcagcct gcatcagcaa cacatccgga gcgccattgg tcgcgcggac
     3541 cgggcgctca tctccggctg aatccgctgc ttctaccgtg gatcgcttgg acgcagtcac
     3601 gttcatactc agatggtgga gtgcgcacgt gcggcggggc ggatggtgag aagaaaaatg
     3661 ctcaggccgt atcggtgcat aaaattgatc tcaccacaga tttgcaatca agctggcctc
     3721 cgcctgcggg cgcacacttc gtggaacgat cacggtgcgg ggcgattccc cctcgaccac
     3781 gaatgaaaga ttcccgattg gcacatccac gccgaaggtc acgtgcacat gtaggcgtga
     3841 ggcgggcccc cagtgcgccc cgattcgaga accaatcccc cacatccaat ggttctgatc
     3901 aggggttccg gcgagcgtgg cttgcgtgct gggcgtgcgc cgcgtgaccc aatccgaaaa
     3961 tcccgtcacc tccacgagcg gcgcgagggt accggcggct tcgtaaccga gacccaatac
     4021 gaggggatga cgttgaagcg agaggtgaac gccttcccga tcgagctcga gttcggggag
     4081 gatcgcatag cttgcgagca aagaccaacg ctccgcaagg tcggtgcgtg cgcccaacac
     4141 caagccggat tgaaaggtgt cttgcgcgaa agacgtgccg agatagccga gcgaaaccgc
     4201 ggtgtgtcgc accgattggg aaggtgcgct cgcccttggg ggtggcgcca cttcgccgac
     4261 gggcgtgcgc tcggaatccc gcaacgtgtg atcatcggag gtgggactcg gcgcgctcgg
     4321 ttccatgccg atatgacgcc cgtcgagcaa ggcctccacc agtgacgaga ccacgatgcc
     4381 ggcctcttct agggcgactc gacctcctgc cgtcttgggg cgaatgcgcc gcaggagcgt
     4441 cgcttccccg ctcacctccg taaagaaaat cagcagctcg ccgcgcggcg tggcatctat
     4501 cgcgaagcta cccagagcct ctcgagaagc cgcttgctga ctcgcctgat ccagccactc
     4561 gttcatgccg gccgcgagtg aaatgcccac cacgtctacc gagacccgca gtccgctcag
     4621 gtgagactcg atggcttgaa ctgcggctag ggctcgttcg cgagtcggat cgaagaccaa
     4681 cacgactcga ggcacgtcag cggcctgggt cgcctcagga tttcctgcgg ccaagcaggg
     4741 cgcggttgcg agcgagctga atacgagcgc tgccagcagg ctgaaatgga ttcgattcac
     4801 gagcggctcg agccaggagc ttaccttgcc ctcctagcgc agccaacggc cgccgcgctc
     4861 gtgtggatgg agctccgctg accgcttcga ggccggcggc tcggcggcgt ctcgtcgggc
     4921 gaggagtacg agtggctacg acggagctgc ctcgatcacc ggataggcac ctgcgtttgg
     4981 cagccgaccg agtagtagag ctgggcgccc ggggtgctgg ctatcgcgca gctcgttgga
     5041 cagagcagga tttgtcgcgg attcgccggg tcatcgtagt accaaccccc ggtcgaagag
     5101 cagtcggagc tcgagcctac gtacaatgtg ctctgtgcgt tgccgtctgc atcgcacagc
     5161 ccgagattca cttcttgggt atccaggtcc cgaccgctcg gcgcgggcgg gatctcgagc
     5221 tggcaaggaa tgcttgcttt cccccggatc gcgttgagtg cctccagcat cattcgggcc
     5281 acatcaccgc cctccaccag gtaggcctct tgggtgcctc ccgcggccgc gatttgatcc
     5341 aaattggaca aggcctggcc aatgccgagc acataagtct tgatcggcac aggtcgggag
     5401 ctgcattccc gagcggccgc taccgcatcg gttagcgtgg ccccacaatt ggtgagcggc
     5461 gtctccggaa tcccgtcggt gacgagcagc agcgccaccg agcggcctgg tcgctcgctt
     5521 cgtgcacggt cgatataggt gcacgcgccg cgcaacgcgg ctcccgttgg cgtctctccc
     5581 gtcggctggg ccacctcgag agaggcaatc aaggcttcgg cttgagcatc gccgatgggg
     5641 agatcgggtg tctcgtaatc cgttggccgg cagctcgcct gcccgagcgg ccgttggcca
     5701 aagtaaccca aaccgagacc aatccccgcg cttcgtggat ctctgagaaa agctgccata
     5761 gcctgacgca ccgggagaat gcgcgcgttc gggtccccca tgcagcagcg gccggggccg
     5821 cccaagctga tggggcatga catcgaacag gactgatcga aaagtacgta tacatcgagg
     5881 ggcacggtct cgcccgcgta ctgctcgccg acacaacccg cgtcgtctgg ctggggcatg
     5941 catgacgggg acgacggcaa ggacgtcgca gaaccgctgc tgaatccgcc caccccgccg
     6001 ggattgggcg ctccgatcgc gcctcccgag tttccagagg gcgtaggcag ccgtggagag
     6061 gttccggcgc cgggacccga ggtgccctcg tcttcgttgg ccgtgactga acagctggag
     6121 agaagaagaa gcgcactcag gctcgagaac gagagcgcac cacgcgatag cgcgacgcca
     6181 atgtgcatcg gagagacctc gggtaggaac acgagctgcc gcggggcagc ccacatgatc
     6241 cgtctagtgg cccggagcct gctcgtcggg tggggacacc tctgacccag acgtcgatcg
     6301 cgagctgggg ggcgcttttt ccagcaagta gtcactcggc gcgcggccga agcgcgcgcg
     6361 gaatacgcgg tgaaactgcg cgtagctgcc gaaacccgct tccaacgcgg cctgcagcag
     6421 gttacccggg tgagcgccag cgcgttccag gaacgacgcg agacggagct cgttccgata
     6481 gtccacgatc gagctcttgg cctccttctt gaaagagcgg gccaagcctc gggggctcaa
     6541 gttcaactgg cgcgccagcg cgtcgcagct gagcgtcgga tcttgggtga gtagcctcgc
     6601 gaccctcctc acgatctctg gctgaggcgt ctcccgttgc tccgtggggc tcacgtccgt
     6661 agtcttcaat cgccacagcg cattcgcgag aatcgagcac agaatggcgg cccgccactg
     6721 ccgcgattca tcgagcggat ggtgcgagat ggccgtgtca ttgaagaaga tcccgagcgg
     6781 ccccccgggc ccgacgatcg ccgtacacgc cacccaaccc tgcccgatca cctgtgtttc
     6841 ttcacccacc tgcgcgatgt gcgggcagtc ctcgtaggcg gtccacggaa aaccggcttg
     6901 agcccgcgcg aacacctcgc gatcgat
//
LOCUS       DASTKU010000441        20602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_84131,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000441 DASTKU010000000
VERSION     DASTKU010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 20602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20602
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84131"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VFQ61_15080"
     CDS             <1..492
                     /locus_tag="VFQ61_15080"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HET9955832.1"
                     /translation="SDSLSIDDAAILANVSATVWSIEALLLCIHVIANLFWIGSITAV
                     GLLVAAGASAGSAADLAVERGRLARLVYQRLATPAFGISFLAGVARLVLNLQYYFVAS
                     HFMHGKLTFALGVIALHHVIGARAKKLAAGQLDASRGAGTLTWACALLAVGAAFFVLI
                     KPF"
     gene            complement(543..1286)
                     /locus_tag="VFQ61_15085"
     CDS             complement(543..1286)
                     /locus_tag="VFQ61_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955833.1"
                     /translation="MKSRVAWVGVGVLGGWLFASGEAGAQAAQPPPPAPSPAAAPAPA
                     GSTATPAAPSSAPPATAIPPGYKLVPIEPPSTGEPPPASTRYDVQYPQRRGALPPGME
                     LPYEAGRPIPPGYRVVKQTRRGLIIAGSIVTGVPWTFSVMGAVGADYDNHSGFLLVPG
                     LGPWLMLAAGGGKDKCNTDYDYRVCENRAGLRAVLVLDGLVQTAGVAMFVAGVAVPKL
                     RLVRNDVTVSMLPTQLGRDGYGLTAIGSF"
     gene            1662..2579
                     /locus_tag="VFQ61_15090"
     CDS             1662..2579
                     /locus_tag="VFQ61_15090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9955834.1"
                     /translation="MNNKPRSRPSGAPRTLPATDRPNLTGEVMKRIKAPHRPVLVVLS
                     GTDVGKRVLVERSLVIGRDPEADLVLSDALVSWHHARLEDRGDTWTLMDLDSTNGISV
                     NGEPCREANLFPNDRIVFGSTIVSFELQDSLQQAYNENVQQLLNIDDLSGLYVRRKFD
                     TELERLVEAARKQASPLSMLVMDLDGVKTINDTHGHLFGAYVIGEAGRVIGRVVRSPA
                     IACRFGGDEYLAALPSVGIDGAVELAERILSSIEGHRFVREGVTLKPGISIGVASFPD
                     DAGTAQELFEAADKALYRAKQAGKHRVCR"
     gene            2805..5327
                     /locus_tag="VFQ61_15095"
     CDS             2805..5327
                     /locus_tag="VFQ61_15095"
                     /inference="COORDINATES: protein motif:HMM:NF023454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HET9955835.1"
                     /translation="MASEYPLYDCLPPVDETNADSILEGFMRYVEGVGLSLYPHQEEA
                     ILALLDDNNVILHTPTGSGKSLVATAVHFKAVAEGKRSFYTCPIKALVSEKFFALCRE
                     LGPDRVGMITGDAAVNRDAPVICCTAEILANIALEGGENADVDYVVMDEFHYFADRDR
                     GVAWQVPLLTLPQARFVLMSATLGDVSVFERALLRLTGQETVTVRSATRPIPLEFEYR
                     EDALHEAVQFLVKQGRAPVYVVNFSQRAAVEAAQSLLSLDFCTKEEKKAIAEELVGES
                     FKSPYGKDVQKFLRHGIGIHHAGLLPRYRLLVEKLSQKARLKIICGTDTLGVGVNVPI
                     RTVLFSKLCKFDGDKVKILSVRDFHQIAGRAGRRGFDERGYVVALAPEHVVENLRLEA
                     KARAGKQKKFVRRKPPEWGYAHFDKSTFDRLVSSEPEPLVSRFEVSPAMLLHVLRREQ
                     GGCRAMKDLVKDSFDTQHQKRQHKKHALSLLRSLWQAGVVDLRPYSEGGGVRVNAELQ
                     EDFSLLHALGLYVLDAVERLDKESPTYALDVVTLVEAIVEDPEVILRAQVDRLKGQKV
                     AELKAAGVEYEERMAELEKVEHPQPNLEFLRDSFALFTKDHPWVMRENLHPKSVAREM
                     LENFQSFSGYVKEYGFLRSEGLLLRYLSEVYKTLVQTVPELARTEELNEAVEFLGTIV
                     RGVDSSLIDEWERMRDPSYRPDSRGDGAAEPAFHDITEQRRTFTALIRNLMFSFLRAI
                     EQRDYESARDLVEAGESFTPAELERVFSPLFEAGQAIDLGPQGRSPRNTQVDVGEYHW
                     DVTQNIMIEGEVSEYTVRGRVDVERSRAAKRPIFVLDHAGAE"
     gene            complement(5355..6305)
                     /locus_tag="VFQ61_15100"
     CDS             complement(5355..6305)
                     /locus_tag="VFQ61_15100"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9955836.1"
                     /translation="MRILGLLLVGLSLGGCDQQPRQALAGTIGSAAASRTRDNAARAD
                     ARDNAAPGAAGRAPAAAAAPPRASDFEQPAIDARTGCPEGMAPIANYCVDRFEAHLWR
                     EQADGKLELHPAHERPLGETRFFARSRERVRPQAYISQVEAALACQNAGKRLCSAAEW
                     YEACQGSKHTTYPYGSHFERRRCNVGKPHLLSRLHGANPGAWRYAEDFNDPRLDQEPG
                     FLALTGEYADCASDYGVYDMVGNLHEWVGDRATADLAKVIPLKAEIRNSLRRRGGNGV
                     FLGGFFSTTSEHGPGCRFATVAHEPTYHDYSTGFRCCRDK"
     gene            complement(6440..6775)
                     /locus_tag="VFQ61_15105"
     CDS             complement(6440..6775)
                     /locus_tag="VFQ61_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955837.1"
                     /translation="MVPLFFSCVCDYCENPRFDGFFRGYVVWRGRAGESDYVFPTREY
                     AERFQRARGLYQNEIRLVLSRDPFHFRKSTGTLKDLEFADRLFEIYPDHRFQPGPGRA
                     FLSPASMTC"
     gene            7235..8890
                     /locus_tag="VFQ61_15110"
     CDS             7235..8890
                     /locus_tag="VFQ61_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9955838.1"
                     /translation="MDKNIWLKSYPAAVPHEIDPDRYRSVVAIFHETCERFADKPCFS
                     NMGVTLTFRQIRELTAQFAAFLTHDLGLKKGDRIALQMPNVLQYPVALFGALRAGLVV
                     VNTNPLYTPREMVAQYRDAGVKAIVILANFASKLAEGLEEIGRPHVIVTELADLFPTV
                     KRVVVNAVVKHVKKMVPAYHLPEVISFRDALARGAARPAQDCPLGPEDLAFLQYTGGT
                     TGVSKGAMLTHRNIVANVLQAATWMGPRFEEGKEVVITPLPLYHVFSLTVNCLLFMKY
                     GGHNVLVTNPRDFKDFIKLLQRERFTVMTAVSTLLNALMNQPGFERIDFSNVKLTVAG
                     AMSLSRSVAERWRKETKSTIIEGYGLTEASPVVCCNPVDGSDRLGTIGLPFPSTDIRL
                     VDDAGADVDGVGSGELVVRGPQVMRGYWNRDQETKNVLHDGWLYTGDIASIDADGFLS
                     IIDRKKDMILVSGFNVYPNEVEEVVLQHPKVLEVGAVGVADEKSGEVVKVVVVRKDPG
                     LTEEELKHFCRERLAGYKMPKVVEFRSELPKTNVGKILRRALR"
     gene            8930..9148
                     /locus_tag="VFQ61_15115"
     CDS             8930..9148
                     /locus_tag="VFQ61_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955839.1"
                     /translation="MDELVNIIVQKTGISESQARTAVETVAGFLKSKLPAPLAAHVDT
                     ALSSAGAAAGNLDLGSLAGGLGGLFGKK"
     gene            complement(9154..13896)
                     /gene="mukB"
                     /locus_tag="VFQ61_15120"
     CDS             complement(9154..13896)
                     /gene="mukB"
                     /locus_tag="VFQ61_15120"
                     /inference="COORDINATES: protein motif:HMM:NF003422.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HET9955840.1"
                     /translation="MTERARATSLILVNWKGVFYERYALDRHVTALEGANGAGKTTVM
                     IAAYVVLLPDLTRLKFTNVGESSAAHGDRGVYGRLGEGARPAYAAMEFVLPNSQRVIA
                     GVLLERKAEPSLSLSPFLISGQDLGARVRQLFLLERDDHEFIPELKELRESVKALGGT
                     LESFASTRDYFARLFELGVTPLRLTTDEDKNKLNEMLRTSMTGGISRALTSELRNFLF
                     KEESGLSDTLSRMRENLEACHRTRIEVAEARRLEHEINGIFETGQAMFAASVWASRKH
                     AEEARAALLEAEAARQQAAAALVALNDSVLEVNTRRQSILERCEVARKALEQADRQCE
                     RAARAQALRGQQQQLQADLARARAELAAREQEHAQRLELRKGLRAERDGAREAYDRAA
                     HGLAHVQAGLAELHKNAHARRHALEQLMEARAMLARPEFEVDEIDGELERLRTRLNEL
                     DAEGARSSRELDSFAARERDYERALSALNEIAEVPSETDAFEHARAALARAATQEHEL
                     ARAPELARELERLRELARRQQRARERAEKLGLSLPAQALEVEKQLVETEERMRALERE
                     VHSEATEADGAQRQIAALEHKLERLALESSRFSELRLLCNRVGLEPPWSRERLLALRK
                     ELTREREACELERINQAIERERLQAELGGLTGGSGANRELSLLCDELDAELYANRFED
                     LEPAEARVMEARLGPLSEALVVEDPAAAAQRVAGQPRERDTVWLLPPHVEPRLPASRG
                     EQERDLIIEEAEGFRVTRLPTEPRLGRRARERRASDLRERIQALTSAAEQTLARAQRA
                     ESTLRDSDAILEHGDLLGAVDPEQTRALTERELQLAQQRERQHRERGEAARLAREALH
                     PRLESLRALLAESFLLEDEAYAERAAECEAQLRAAHAAESNLRRSANARRVLGELLDV
                     LRGPRPTEQARAELRSEFERKNLERDRAFRAQRALQELKRCRPALAFEDAESALAEQR
                     AVVPALEAQLETAKTAAARAEAELSRAEATWEESVTSRQSAEADVQALLAHISRVEVE
                     LSSEAEAGALYVDEERARAEAIERSREYTALDREERTLSTELALREERKSRVQRMAEQ
                     AEADCNARQSEAEPRSRYAEELARSAEAAGILTRALSQRFESELAPQGSERLRVLCQG
                     KASQLLDRLRSARGGQELANLVENALRDKALHESLLPLKLWLEVRNWLQSRLPVPIGG
                     SVEPLESLAELRDGLTLLEQRLTLQERDLTGTSEDVARSIDVQLRRAKAQVRRLNQNL
                     EGIRFGSIAGIRVQMRRIERMDQVLTALREGSAQSLLFQSALPIEEALDELFRRYAGG
                     RSGGQRLLDYREYAELIVEIRRQSRDDWEAASPARLSTGEAIGVGAALMMVILTEWER
                     DARLLRGKRDGGSLRFLFLDEANRLSQDNLGVLFDLCQSLDLQLLIAAPEVAKAHGNT
                     TYRLVRRINEDGQEEVLVSGRRTIEERGPEPDEPSAEETAQRTLALIPELPFMSEPEL
                     PFMSEPAQPRAAIEFTPEVAATAHETSEADGTEPVRSEPEALESTPHEPETLQAAPPE
                     AAAPDRVQLGLLGPVAEPSFSGTDSENS"
     gene            complement(13893..15053)
                     /locus_tag="VFQ61_15125"
     CDS             complement(13893..15053)
                     /locus_tag="VFQ61_15125"
                     /inference="COORDINATES: protein motif:HMM:NF016198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HET9955841.1"
                     /translation="MNSSRFERLEDAIQHECFPDVDLALRRGRHIDRSDEAWFTFLSD
                     AAEHLDPLYRRFECELVHKSDGYYYLLPTGDKISRRQLSVAEMLVGQALTLLYLDTAS
                     LEHGGVVTREQLIGQLAGTLGSDALIKLLNPKRKRYDERVAEETVRSKIAEAVRRLST
                     LGFVDVVEDSAIRLRPALLRFAEPVRGNAAPEAALTRLLEKGELSYIDADGSEPDPNV
                     EGDDELGPEDPEMSDEEDSPEEERAARDTSSLSDERRPGQHTESHSERVGDSEPALDS
                     ERAGDSEPALGSELAVRDPEFPPDRSEAENASLFAEWADIGEEPERPRMLEKPSPDAG
                     LEDAVELEPELGGGFPADDGGTASEPPPLDDLDSAAEGTAPEREPEPEEDAS"
     gene            complement(15050..16354)
                     /locus_tag="VFQ61_15130"
     CDS             complement(15050..16354)
                     /locus_tag="VFQ61_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensin subunit MukF"
                     /protein_id="HET9955842.1"
                     /translation="MSSPAAPEPNQALLALAERNVRLELETLDICFLAALYLRAERAQ
                     LSAFDEAQLFEVFEQVALVLDPSAGNAQKRAGAALRRAREQRLLARVDGAGLSRAGEY
                     ALTRLSLGIVEFFLQDESLTRESLTVLTRTLLASLTEVARVAADLNESSDFQALVVAP
                     LSITVQELLSGIERRQRGFDLEQERLKREIGELVRADWFGAVERCQTLLDTTSQTLGE
                     LNQVLLRDTHAIMTLLQDIDEAAARAGRDEADRTLRAIMDQIDRIAAWGSARQRAWSE
                     YYQYVHRYLRDVVRLDPSRTLIQRLREQLSGKGGRRYALCVAAAAPARVLREVLPPIE
                     RPPVRRKKKPVEAAAETVEASDPDADLQARVVIALGEGKRSLAEITAQLTEDETPSQR
                     FLAAGKIAETVARVAQPREVSERPWVSVGGGVSIEDWPELDP"
     gene            16718..17245
                     /locus_tag="VFQ61_15135"
     CDS             16718..17245
                     /locus_tag="VFQ61_15135"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET9955843.1"
                     /translation="MNVTTRPEDAQALAEALTTHCCSELLSDFGFEPVWLAPEHAVPD
                     GVSFCSVVGFSGQKLRGSIIMSSSGRAANELSSHIPGPIRDVIGEIANQLGGRLKNKF
                     LLYGVDLYMTLPVVLRGEHFAPMPRIELRPIGFSIGSGSMWVWVDTECFDGFEMAVRP
                     NPAEAPMAEGEALLF"
     gene            17351..17722
                     /locus_tag="VFQ61_15140"
     CDS             17351..17722
                     /locus_tag="VFQ61_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9955844.1"
                     /translation="MSPKVLIVDDSATVRQQVRLALTQAGFDVIEANDGLEGLDKINS
                     ASDIKAVICDVNMPRMNGLEMLERCHTQGGPRMPVLMLTTEGKPALIQKAKEYGAKGW
                     IVKPFKADLLVAAVKQLTRAA"
     gene            17730..18956
                     /locus_tag="VFQ61_15145"
     CDS             17730..18956
                     /locus_tag="VFQ61_15145"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9955845.1"
                     /translation="MPLRGVARVFSRWFGRGSRPASDTEDSVPPETDLRSGVMRSVPP
                     FLPTNDLDGSAFGVEATPGFGMGAAPVGLDALAENEVPVPEAPPPSEPGTPLLELVRE
                     TFTQCRNNVLSRIQSVNAMTAREVIAVGDSLQAIVGRAQAQVGATRDTLKGLSGQGRA
                     GVAEIIERQTQMVTTHLSTIHAALEQQTELTRQALVASSGIAAIGEQVSKVALQTRLL
                     SLNANVEAARLGANGAGFEVIASQMRRLTDEIDQANRQIGQMAQALLVSLPQIQKHGE
                     RLNRSAEAFSKDLAENSSQVYGATAHLKTSLESVLARGDHEAGLILQGSYEAISHLQF
                     QDPTAQSLLIIDADLARLMERVTTLLEASRTGSVSVTNHSFENLEAAESHPELHAGEV
                     VSLGSEPLATGDVMLF"
     gene            19161..19583
                     /locus_tag="VFQ61_15150"
     CDS             19161..19583
                     /locus_tag="VFQ61_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9955846.1"
                     /translation="MPTARELMTPGPVTVPVTAKVQKAVEILHTAEVRHLPVVDEEGN
                     LVGMLSDRDLRALSLPVFVGDEHVASLRTALNAPVSTLMNSDVVSVESEADVSEVIEL
                     MLDNKIGAVPVTDADGLLTGIVSYVDILRALPFETEGD"
     gene            complement(19727..20128)
                     /locus_tag="VFQ61_15155"
     CDS             complement(19727..20128)
                     /locus_tag="VFQ61_15155"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9955847.1"
                     /translation="MSMDVGSKKGPRSDINVTPLIDVVLVLLIIFMVLTPSMLKHLTA
                     TVPRKAEDNTPPSTDTTIVVEYTAKRELSVNTEPISIEELTEKLKARLRGGRQKVVFF
                     KAEDEAPYGEVIRIMDIARGSGAEVLAVVTK"
     gene            complement(20196..>20602)
                     /locus_tag="VFQ61_15160"
     CDS             complement(20196..>20602)
                     /locus_tag="VFQ61_15160"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9955848.1"
                     /translation="EREPRKVMSDINVTPLVDVVLVLLIIFMVVTPMIASGVAVDLPR
                     TKFHSRKPDDGKDIIISITSDKRIFVGGNRVNRVQDLGQAVIAERRKTPEKTIFVKGD
                     TRVPYGTVREAMDVLHDADIEDIVLGTEELKN"
BASE COUNT         3562 a         6643 c         6592 g         3805 t
ORIGIN      
        1 tccgattcgc tgagcattga cgacgccgcc attctggcga atgtatcagc caccgtgtgg
       61 tccatcgaag ccctcttgct ctgtattcac gtaatcgcga acctgttctg gatcggctcg
      121 attacggcgg tgggcctgct cgtggcggcg ggcgcgagtg cggggtcggc agctgacctc
      181 gccgtcgagc gagggcggct cgcgcggctc gtctaccagc gcctggcgac gcccgcgttt
      241 ggcatctctt tcctggccgg cgtggcccga ttggtgctga acctccaata ctacttcgtg
      301 gcttcacact tcatgcacgg caagctcacg ttcgcgctcg gtgtgattgc gctgcatcac
      361 gtgatcgggg cgcgcgccaa gaagctcgcc gcgggtcagt tggatgcaag tcgcggcgcc
      421 ggcaccttga cctgggcctg cgccctcctc gcggtgggcg cagccttctt cgtgctgatc
      481 aagcctttct gagtttctgt ccgagttctc gccccggcga ttcgtctgcg gcgtgaaaaa
      541 cctcaaaagc tgccgatcgc cgtcaagccg tagccgtccc ggcccaactg cgtggggagc
      601 atggagaccg tgacgtcgtt ccgcaccagg cgcagcttgg ggaccgccac tccggccacg
      661 aacatcgcaa cccccgcggt ctgcacgaga ccatcgagca ccaaaaccgc gcgcaagccc
      721 gcgcggtttt cacacacgcg atagtcgtag tcggtgttgc atttgtcctt cccgccgccc
      781 gcggccagca tcaaccacgg cccgagccct ggaacgagca aaaagcccga gtggttgtcg
      841 taatcggcgc ccaccgcacc catcacgctg aaggtccaag gtacaccggt cacgatcgag
      901 cccgcgatga tcaacccgcg tcgagtctgc ttcaccacgc gatagccggg cgggatcggt
      961 cggcccgctt catagggcaa ctccatgccc ggcggtaagg cgccccgccg ctgcggatac
     1021 tgcacgtcat agcgagtcga agcaggaggg ggctcgcccg tacttggcgg ttcgatcggt
     1081 accagcttgt agcccggcgg aatcgctgtc gccggtggcg ctgaactggg cgccgcgggt
     1141 gtagccgttg aacctgcggg tgcaggcgca gctgcgggag atggggccgg gggcgggggt
     1201 tgggccgcct gtgcacccgc ctcgccactc gcaaacaacc atccacccag aacgcccacc
     1261 ccaacccacg caacacgaga cttcatattc atacccacct agcaagctgc ataccgcgaa
     1321 aggtggtgtc tgtcaccgct ccgagcactg cacgaatccc gcccaatcgt gcgcgtcgag
     1381 cggctcgagc gcagccaaat agtgggctct cggtggggcg ttcgagtcac agtgaatgca
     1441 cggcccaggt tggcttctac ggtgagcgcc tcgcgggcca atgagtttgc ccacgcctcg
     1501 ccgtgccccg tccggccact ccctctagac ggccgtctct ctgctggata tcggagccga
     1561 gcgcggatgt cgccgggaat tgcctgaagg caccgcccca aaaccgaaaa acggactcag
     1621 gctcgtctgt gcgcgaggcc tgagatacgc ttctccacac gttgaacaac aaaccgcgca
     1681 gtcgaccctc cggagcgccc cggacgctgc cggccaccga tcgcccgaac ctcacgggcg
     1741 aggtgatgaa gcgaatcaaa gcgccgcacc ggccggtgct ggtggtgctg agcggcactg
     1801 acgtcggcaa gcgcgtgctc gtcgaacgat cgctcgtgat cgggcgcgat cccgaggctg
     1861 atctcgtgct ctcggacgcg ctcgtctctt ggcaccacgc gcgcctcgag gaccgaggtg
     1921 acacctggac gttgatggac ctcgatagca ccaacggcat cagcgtaaac ggcgagccgt
     1981 gtcgagaggc caatctcttc ccgaacgatc gcatcgtgtt cggcagcacc atcgtctcgt
     2041 tcgaactcca agacagcctg caacaggctt acaacgagaa cgtccagcaa ctcctcaaca
     2101 tcgacgatct ttcggggctc tacgtgcgtc gcaagttcga caccgagctc gagcgactag
     2161 tcgaagccgc gcgcaagcaa gcctccccgc tcagcatgct cgtcatggat ctcgacgggg
     2221 tcaaaaccat caacgacacc catgggcatc tgttcggcgc gtacgtgatc ggcgaggcgg
     2281 ggcgcgtgat cggtcgtgtc gtacgtagcc ccgcgatcgc ctgtcgtttc gggggggacg
     2341 agtacctcgc cgcgttgccg agcgtcggca tcgacggcgc cgtagagcta gcggagcgca
     2401 ttctaagcag catcgagggc caccgcttcg tgcgtgaagg ggtcaccctg aagccgggta
     2461 tctcgatcgg ggtcgcatct tttccggatg acgccggtac ggcgcaagag ctgttcgagg
     2521 cggcggacaa agcgctctat cgagccaaac aagccggcaa acaccgcgtt tgccggtgag
     2581 ctttcgggcc gcccgtgact acgagcgcgc tgcacgccag cctgcggccc gcgcgcggcg
     2641 agtctcccgc gagggaaccc accgcgccgc gcaagcggca aggcgtaacg cccgtctaac
     2701 gacgcggtcg cgccacggga aaacacacga ccctcaccgg acgggctggt tttctcgcga
     2761 acccctttac tcgccgggcg gctcgtgcga gcccgacgag cgagatggct tccgaatatc
     2821 ccctatacga ctgcttgccg ccggtcgacg aaaccaacgc ggacagcatt ctggaaggct
     2881 tcatgcgtta cgtggagggc gttggactgt ccctctaccc gcaccaagag gaggccatcc
     2941 tcgcgctgct cgacgacaac aacgtgatcc tgcacacgcc caccggctcc ggcaaatcgt
     3001 tggtggcgac ggcggtgcac ttcaaggcgg tggcggaggg caaacgctcc ttttacacct
     3061 gcccgatcaa ggcgctggtg agcgagaaat tcttcgcgtt gtgccgagaa ctcgggcccg
     3121 accgcgtcgg gatgatcacg ggtgacgcgg ccgtcaatcg cgacgccccg gtgatctgct
     3181 gcaccgccga gattctcgcc aatatcgcgc tcgaaggcgg cgagaacgcc gacgtcgact
     3241 acgtcgtgat ggacgagttc cattacttcg cggatcgcga tcgcggcgtg gcttggcaag
     3301 tgccactgct cacgctgccc caagcgcggt tcgtgttgat gagcgccacg ctgggtgacg
     3361 tgtccgtctt cgagcgcgcg ctgctgcggt tgaccggcca agaaaccgtc accgtgcgct
     3421 cggcgacgcg ccccattccg ctcgagttcg agtaccgcga agacgcgcta cacgaggccg
     3481 ttcagttcct ggtcaagcaa ggtcgcgccc ccgtttacgt ggtgaacttc agtcagcgcg
     3541 cggccgtcga ggcggcccaa agcctgctga gcctggactt ctgcacgaag gaagaaaaga
     3601 aggccatcgc cgaggagctg gtgggggaga gcttcaagag cccttacggc aaagacgtgc
     3661 agaagtttct gcgccacggc atcggcatcc accacgcagg gttgttgccg cgctatcgct
     3721 tgctcgtcga gaagctgtcc cagaaggcgc ggctcaagat catctgcggc acggataccc
     3781 tgggcgtggg cgtgaacgtg cccattcgaa ccgtgctgtt cagcaagctg tgcaagttcg
     3841 acggggacaa ggtgaagatc ctgagtgtcc gcgattttca ccaaatcgct gggcgtgcag
     3901 gccgtcgtgg tttcgatgaa cggggttacg tcgtggcgct cgctccggag cacgtcgtcg
     3961 agaacctgcg gctcgaggcc aaggcgcgcg ccggcaaaca aaagaagttc gtgcgtcgca
     4021 agccgcccga gtggggctac gcgcacttcg acaagtcgac cttcgatcga ctcgtgagct
     4081 cggagccaga gccgttggtg tcgcgcttcg aggtctcccc ggcgatgctg ctgcacgtgc
     4141 tccggcgcga acagggcggt tgccgggcca tgaaggatct cgtcaaagat agcttcgaca
     4201 cgcagcacca gaaacgccaa cacaagaagc acgcgctctc gttgctgcgc tcgttgtggc
     4261 aagccggggt cgtggatctg cggccgtact cggagggagg cggggtacgc gtgaacgccg
     4321 agttgcagga agacttctcg ctactgcacg cgctcgggct atacgtactc gacgcggtgg
     4381 agcggctcga caaggagagc cccacctacg cgctcgacgt ggtcacgctg gtggaggcga
     4441 ttgtcgaaga ccccgaagtc atcttgcgcg cgcaagtcga tcgtctgaaa ggccaaaagg
     4501 tggcggagct caaggccgcc ggcgtggagt acgaagagcg tatggccgag ctcgagaagg
     4561 tcgagcaccc gcaaccgaac ctagagtttt tgcgggacag cttcgcgctg ttcacgaaag
     4621 accacccgtg ggtcatgcgc gagaacttgc accccaagtc ggtcgcgcgc gagatgctcg
     4681 aaaattttca gtccttcagc ggttacgtca aggagtacgg tttcctgcgt tcggaagggc
     4741 tactgctgcg ttacctgtcc gaggtctata agacgctcgt gcaaaccgtg cccgagctgg
     4801 cgcgcacgga ggagttgaac gaggcggtgg agttcctggg caccatcgtt cgcggggtgg
     4861 actcgagctt gatcgacgag tgggagcgca tgcgcgatcc gagctaccgc ccggattctc
     4921 gcggcgacgg cgcggccgag ccggccttcc acgacatcac cgagcagcgc cgcacgttca
     4981 ccgcgttgat tcgcaacctg atgttctcgt tcctgcgagc gatcgagcag cgggattacg
     5041 agagcgcgcg cgatttggtc gaagctggcg agtccttcac gcccgcggag ttggagcggg
     5101 tcttctcgcc gttgttcgaa gcgggtcagg ccatcgattt ggggccccaa gggcgctcac
     5161 cacgcaacac gcaagtggat gtcggcgagt accattggga cgtcacccag aacatcatga
     5221 tcgagggcga ggtgagcgaa tacaccgtac gcgggcgggt cgacgtggaa cggtctcgcg
     5281 cggcgaaacg gccgatcttc gtgctcgacc atgcgggggc ggagtagcga gctccggctt
     5341 cgcgtcaggg ccggctattt gtcgcggcag cagcggaagc ccgtcgagta gtcgtggtaa
     5401 gtcggttcgt gcgcgaccgt cgcgaaccgg cagcccgggc cgtgttcact ggtcgtgctg
     5461 aaaaaaccac ccaaaaacac gccgttcccc ccgcgccgcc gcagtgagtt tcgaatttcg
     5521 gccttgagcg ggatcacctt ggccaaatcg gcagtcgccc ggtccccgac ccactcgtgc
     5581 aggttcccga ccatatcgta aacaccgtag tcactcgcgc agtcggcata ttcgccggtg
     5641 agcgccaaga aaccgggttc ttggtcgagc cgcgggtcat tgaagtcttc ggcgtaacgc
     5701 caggcgcccg gatttgcgcc atgcaggcgc gacagcaagt ggggttttcc aacgttgcag
     5761 cggcgacgct cgaagtgcga gccgtacggg tacgtggtgt gcttggagcc ttgacacgcc
     5821 tcgtaccact cggcggcact gcacaagcgt ttgcccgcgt tctgacatgc gagcgcggcc
     5881 tccacctggc tgatgtacgc ctgcggtcga actcgttcgc gggaacgagc gaagaagcgc
     5941 gtctccccga gcggcctctc gtgggcgggg tgcaattcga gcttgccgtc ggcctgctcg
     6001 cgccaaaggt gtgcttcgaa acgatcgaca cagtagttcg cgatcggcgc catgccctct
     6061 ggacaaccgg ttcgggcatc gatcgccggc tgctcgaagt cactcgccct aggcggggcc
     6121 gcggccgcag ccggcgcgcg gccagctgct cctggtgccg cgttatctcg agcgtcggct
     6181 cgcgcagcgt tgtcgcgcgt tcgcgatgca gccgcggagc caatggttcc cgccagcgcc
     6241 tgccgcggct gctggtcaca tccgcctagg ctcagcccaa ctagcaacaa gccgaggatg
     6301 cgcacctggc tagcgtagca gccgcgcccc tggcagccac ggcgcgttca gcgccgcgcg
     6361 cggttcgaag cgtcgaaagc gatggcttca gagcaccaaa cctcaaggtt cggctgcgag
     6421 cgcgagccgc gcgcgaactt cagcacgtca tcgaagccgg ggagaggaag gctcgtccgg
     6481 ggccgggttg aaaccgatga tcggggtaaa tctcgaacag ccgatccgcg aactccagat
     6541 ctttgagcgt gccggtgctc ttccggaagt ggaacggatc acgcgacaag acgagccgaa
     6601 tctcattctg gtagagtccg cgcgcgcgct ggaaccgctc ggcgtactcg cgggttggga
     6661 atacgtaatc cgattctccg gcgcggccgc gccacaccac gtatccgcgg aagaagccgt
     6721 cgaagcgtgg gttctcgcag tagtcacaca cgcacgagaa gaacaaagga accatcggtg
     6781 aaccgcagtc gggacacagc atctggcaac cctcgagggt gttattggcg acagatggtt
     6841 accacgcgcg gtcggctttc gccatcgaac ggctgacgga atatccgact cgcgcccggc
     6901 actcccagag ctcgttctcg tccgcagctc gggcgcggct catggtcatg ctttgcgccc
     6961 gttccaatcg caactcgagt cgcgctccta gttggagaag gcgtgggttc agccgtgcga
     7021 gtgacggcac gttcgcgtca ggcaggggac gtgaaccggc tttggcagcc gcgattccgt
     7081 gtcgcggcga gacggaacca gtgcgccgtt ccgcggcgca gcacgggctc ggatttttac
     7141 aggatcttgc gcggctggcc cgttgcgaga cgcggcctgc cgtagttctg ttacgtaccc
     7201 ccaaaccaag ctagagtccc cgtaatcccc gtccatggat aagaacatct ggctcaagag
     7261 ttaccccgcc gccgtccctc acgagatcga tccggatcgc tatcgctccg tggtcgccat
     7321 cttccacgaa acgtgtgagc gcttcgccga caagccttgt ttctccaaca tgggggtgac
     7381 gctcacgttt cgccagattc gcgagctgac ggcgcagttc gctgcgttcc tcactcacga
     7441 cctggggctg aagaagggcg atcggattgc tctgcagatg ccgaacgtgt tgcagtaccc
     7501 ggtggcgttg ttcggtgcgc tgcgagcggg cttggtcgtg gtcaacacga atccgctcta
     7561 tacgccgcgg gagatggtgg cgcagtaccg tgacgcgggg gtcaaagcca tcgtgattct
     7621 ggccaacttc gcgagcaagc tcgcggaagg gctcgaagaa atcgggcggc cccacgtgat
     7681 cgtcacggag ctggcggatc tgttccccac cgtgaagcgc gtcgtcgtga acgcggtggt
     7741 gaagcacgtc aagaagatgg tcccggccta ccacttgccg gaggtcattt catttcgaga
     7801 cgcactggcg cggggtgcgg cgcgtccagc ccaggattgc ccgcttggac ccgaagattt
     7861 ggcgttcctt cagtacacgg gaggaaccac gggcgtctcg aagggggcca tgctcacgca
     7921 tcgcaacatc gttgcaaacg tgctccaagc cgccacttgg atggggccac gcttcgagga
     7981 gggcaaggag gtggtgatca cgccgttgcc cctctatcac gtgttctcgt tgacggtgaa
     8041 ttgcctactc ttcatgaagt acggcgggca caacgtcttg gtcaccaatc cgcgtgactt
     8101 caaagatttc atcaagcttt tgcagcggga acgcttcacg gtgatgacgg cggtgagcac
     8161 gctgctcaac gcgctgatga accagcccgg cttcgagcgg atcgactttt cgaacgtgaa
     8221 gctgaccgtg gcgggagcca tgtcactctc gcgcagcgtg gccgagcgct ggcgaaaaga
     8281 aacgaagagc acgatcatcg aaggctacgg cctcaccgag gcttcgcccg tcgtgtgctg
     8341 taacccggtg gatggcagcg accgcttggg gacgattggc ttgccgttcc cgtcgacgga
     8401 catccggctc gtcgacgacg caggtgcgga cgtagatggc gttggttcgg gggagctcgt
     8461 ggtgcggggc ccgcaagtca tgcgcggcta ttggaaccga gatcaagaga ccaaaaacgt
     8521 gctccacgat ggttggctct acaccggtga tatcgccagc atcgacgccg atggattctt
     8581 atccatcatc gatcgcaaaa aggacatgat cctagtttcc ggcttcaacg tgtacccgaa
     8641 cgaagtcgaa gaggtcgtgc tgcagcaccc caaggttctc gaggtcggcg cggtcggggt
     8701 ggcggacgag aaatcggggg aggtcgtcaa ggtagtggtc gtgcgcaaag atccaggcct
     8761 caccgaggag gaactcaagc acttctgtcg tgagcgtctc gcgggctaca agatgcccaa
     8821 ggtcgtcgag tttcggagtg agttgccgaa gaccaatgta ggtaaaattc tcaggcgcgc
     8881 tttgcgctga ggtcgagtat gttcccgcct tcaacagcga ggtgacccaa tggatgaatt
     8941 ggtcaacatc atcgtgcaga agaccggcat ttcggagagt caggcgcgca ccgcggtcga
     9001 gacggttgca ggcttcttga agtcgaagct accggcgccg ctggcggctc acgtcgacac
     9061 cgcgctcagc agcgcgggcg cagccgcggg caatctcgat ctcgggagtc tcgccggcgg
     9121 cctcggaggc ctgttcggca agaagtagct gctttacgag ttctcggagt ccgtgccgct
     9181 gaaagacggt tcagcgacgg gacccagcaa cccgagctgc acgcgatccg gcgcggcagc
     9241 ttcggggggc gccgcttgga gcgtctccgg ctcgtgcggc gtggactcga gcgcttcggg
     9301 ctcggagcgc acgggctccg tcccgtcagc ttcggacgtt tcgtgagccg tcgccgcaac
     9361 ctcgggcgtg aattcgattg ccgccctggg ctgcgccggc tcactcatga acggcagttc
     9421 cggctcgctc atgaacggca gttccgggat gagggcgagg gttcgttgag ccgtctcttc
     9481 agcacttggc tcgtccggct ccggacccct ttcttcgatc gtccggcgcc cgctcacgag
     9541 cacctcctcc tgcccatcct cgttgatgcg gcgtacgaga cggtaggtgg tgttgccatg
     9601 agccttggcg acctctggcg cggcaatcaa gagctggaga tcgaggcttt ggcagagatc
     9661 gaatagcacc cctaggttgt cttgggagag gcggttggct tcgtccaaga acagaaaacg
     9721 cagcgatccc ccgtcgcgtt tgccgcgcag caagcgcgcg tcgcgttccc attcggtgag
     9781 aatgaccatc atcaacgcgg cgcccacccc gatggcttct ccggtagata ggcgggcagg
     9841 ggaggccgcc tcccaatcat cgcggctttg ccgtcggatc tcgacgatca gctccgcgta
     9901 ttcccgataa tcgagcaggc gttgcccgcc ggaacggccg cccgcgtagc gccggaacag
     9961 ctcgtcgagc gcttcctcga tcggcagtgc cgactgaaag agaaggctct gggccgagcc
    10021 ttcacgcagc gccgtgagca cctgatccat gcgctcgatg cgtcgcattt gtacgcgaat
    10081 gccagcgatg ctcccgaagc ggatgccctc caggttctgg ttcaagcgcc gcacctgtgc
    10141 tttggcgcgc cgcagctgca cgtcgatgct acgggcgacg tcctccgacg ttcccgtgag
    10201 atcgcgctcc tgcagcgtca ggcgttgctc gagcagggtg aggccatctc gcagctcggc
    10261 caaggactcg agcggctcca cactcccgcc gatcggaacg ggcaggcggc tctgcagcca
    10321 gttgcgcacc tcgagccaaa gtttcaacgg cagcaggctc tcgtgcagcg ctttgtcccg
    10381 taacgcgttc tccaccaggt tcgcgagttc ctgaccgccc cgcgcgctac gcagccgatc
    10441 gagcagctgc gaggccttgc cctggcacag aacgcgcagc cgttcgctgc cttgcggcgc
    10501 gagctcgctt tcgaagcgtt gtgagagcgc gcgcgtcaag atccccgccg cttccgcgct
    10561 gcgcgccaat tcttccgcgt agcggctgcg cggctcggct tcgctttggc gcgcgttgca
    10621 gtcggcctcg gcctgttcgg ccatgcgttg cacgcggctc ttacgctcct cgcgcagcgc
    10681 tagttcggtg gagagcgtac gctcctcgcg atcgagcgcg gtgtactcgc gcgagcgctc
    10741 gattgcttcc gcacgggccc gctcctcgtc cacgtacaac gcgcccgcct ccgcctcgga
    10801 gctgagctcc acctccacgc gcgaaatgtg agccagcaag gcttgcacgt cggcttcggc
    10861 gctttggcgc gacgtcacgc tctcttccca agtcgcctcc gcccgtgaga gttcggcttc
    10921 ggcgcgggcc gcggcggtct tggcggtctc cagttgtgct tccagcgccg gcacgacggc
    10981 ccgttgctct gcgagcgcgg actcggcgtc ttcgaaggcc agcgcggggc ggcaacgctt
    11041 gagctcctgc agcgcccgct gcgctcggaa ggcccgatcc cgctcgaggt tcttgcgctc
    11101 gaactcggag cgcagctcgg cccgggcttg ctcggtcggg cgtgggcctc gcaacacatc
    11161 caatagctcc ccgagcacgc gtcgcgcatt cgcactgcgt cgcaggttcg attcggcggc
    11221 atgagccgcg cgcagttgcg cttcgcactc agcggcccgc tccgcgtaag cctcgtcctc
    11281 gagcaagaag gactcggcga gtagtgcgcg caacgactcg agccgcgggt gcagggcctc
    11341 gcgtgccaag cgcgccgctt ctccgcgctc tcggtgctgt cgctcccttt gctgggcgag
    11401 ctgcagctcg cgttcggtga gcgcgcgcgt ttgttctgga tcgaccgccc cgaggaggtc
    11461 tccgtgctcg aggatggcgt cggaatctcg cagggtggac tcggcgcgct gagctcgggc
    11521 cagggtctgc tccgcggcgc tggtgagcgc ttgaatgcgc tcccgtaagt cgctcgcgcg
    11581 acgttcgcgc gcgcggcgac cgagtcgcgg ctcggtcgga agacgagtga cccgaaagcc
    11641 ctccgcctct tcgatgatca aatctcgttc ttgttcgccg cggcttgcag gcaagcgagg
    11701 ctcgacgtgc ggagggagta gccacaccgt atcgcgctca cgcggctgtc ccgcgacacg
    11761 ctgcgcggcc gcggcgggat cttcgacgac cagcgcttca ctcaagggcc cgagccgggc
    11821 ttccatgacg cgcgcctccg ccggctcgag atcctcgaag cggttggcgt agagttcggc
    11881 gtcgagctcg tcgcacagca aggacagctc gcggttcgcc cccgagccgc ccgttagccc
    11941 tcctagctcg gcttgtaggc gctcgcgctc gatcgcctga ttgattcgct ccagctcgca
    12001 ggcctcgcgc tcgcgggtga gctccttacg cagcgcgagt agccgttcgc ggctccacgg
    12061 cggctcgagg cccacgcggt tgcacagcag gcggagctcg gagaagcgag agctctccag
    12121 tgccaagcgc tccagcttgt gctcgagcgc cgcgatttgc cgctgcgcac cgtcggcctc
    12181 ggtggcctcg ctgtgcactt cgcgctcgag ggcccgcatg cgttcttcgg tctcgacgag
    12241 ctgcttctcg acctcgagcg cctgcgcggg taacgagaga cccagctttt cggctctttc
    12301 gcgagcgcgc tgctgccggc gcgcgagctc gcgcagccgt tcgagttcgc gcgccaactc
    12361 cggtgcgcgc gccagctcgt gctcttgcgt tgcggcgcgc gccagtgcgg ctcgggcgtg
    12421 ttcgaacgca tcggtctccg agggcacttc cgcgatttcg ttcaacgccg agagtgcgcg
    12481 ttcgtagtcg cgttcacggg ccgcaaaaga gtcgagctca cggctcgagc gcgcgccttc
    12541 agcgtccagc tcgttcaaac gcgtgcgcaa tcgctcgagc tcgccatcga tctcgtcgac
    12601 ttcgaactcc ggccgggcga gcatggcgcg agcctccatg agctgctcca gcgcgtgccg
    12661 ccgcgcgtgc gcgttcttgt gcagctccgc caagccggct tgcacgtggg ccaggccgtg
    12721 agcggcacgg tcgtaggcct cgcgcgcgcc gtcgcgctct gcccgcaggc ccttacgcag
    12781 ctccagacgc tgcgcgtgtt cttgctcacg tgcggcgagc tcggcgcgtg cccgcgccaa
    12841 atcggcctgc agctgctgtt gttggccgcg cagggcctga gcacgcgcgg ctcgctcgca
    12901 ttgccgatcc gcttgctcga gcgcttttcg cgctacctcg cagcgctcca gaatgctttg
    12961 ccggcgcgtg ttcacctcga gcacgctgtc gttcagcgcc acgagggccg cggccgcctg
    13021 ctgccgagcc gcctcggcct cgagcagcgc cgcgcgcgcc tcttcggcgt gcttgcggct
    13081 ggcccagacg gacgccgcga acatggcttg cccggtctcg aagatgccgt tgatctcgtg
    13141 ctcgaggcgc ctggcttcgg ctacctcgat gcgggtgcgg tgacaggcct cgaggttctc
    13201 gcgcatgcgc gagagcgtgt cggacaagcc gctctcctcc ttgaacagga agttgcgcag
    13261 ctcgctggtc agcgcgcgag agatgcctcc cgtcatgctc gtgcgcagca tctcgttgag
    13321 cttgttcttg tcctcgtcgg tggtcagtcg gagcggcgtg acgccgagct cgaacaaccg
    13381 cgcgaagtag tcgcgcgtgg aagcgaagct ctccagcgtg ccacctagtg ctttgacgga
    13441 ctcgcgtagc tccttgagct ccgggatgaa ctcatggtca tcgcgttcga gtaggaagag
    13501 ctgccgcacc cgggctccga ggtcttgccc ggaaatcaga aatggcgaca gcgacaggct
    13561 cggctcggcc ttgcgctcca acaacacgcc cgcaatcacg cgctgggagt tcggtaagac
    13621 gaactccatg gcggcgtacg cggggcgtgc accttcgccc aagcgcccgt acacgccgcg
    13681 gtcgccgtgc gccgcggagc tctccccgac gttggtgaac ttgagccgcg tcagatccgg
    13741 caacaaaacc acgtaggccg cgatcatgac cgtggtcttg ccggcgccgt tcgcgccttc
    13801 caaggccgtt acgtgccggt cgagcgcata gcgctcgtaa aacacgccct tccaattgac
    13861 cagaatgagc gaggtcgccc gcgcacgttc ggtcaagaag cgtcctcttc aggctcgggt
    13921 tctcgctccg gcgcagtccc ttcggccgca ctatcgaggt cgtcgagtgg aggaggctcg
    13981 ctcgctgtgc ccccgtcatc ggcaggaaat ccgccgccca gctctggctc gagctcgacg
    14041 gcgtcttcga gcccggcatc tggcgaaggt ttctcgagca tgcgcggtcg ttccggctct
    14101 tcgccgatgt cagcccattc cgcgaacagg ctcgcgtttt cggcttctga acgatcgggg
    14161 gggaactctg ggtcgcgaac tgcgagttcg ctgcccaacg cgggttcgct gtcacccgcg
    14221 cgttcgctgt ccaacgcggg ttcgctgtca cccacgcgtt cgctgtgact ctccgtgtgt
    14281 tgcccgggtc gccgttcatc ggacagcgaa ctcgtatctc gagcggcgcg ctcctcctcg
    14341 ggcgaatctt cctcgtcgct catctccgga tcttcggggc cgagttcgtc gtcaccttcg
    14401 acgttggggt ctggttcgct gccgtccgcg tcgatgtacg agagctcgcc cttttcgagc
    14461 aaccgcgtca aagccgcctc cggagcggcg ttgccccgca ccggctccgc aaagcgcaac
    14521 aaagcggggc gcagacggat ggccgagtcc tccaccacgt ccacgaaacc gagtgtcgaa
    14581 agccttctca cggcttccgc gatcttgctg cgcaccgtct cttcggccac gcgctcgtcg
    14641 taccgcttgc gcttcggatt caagagcttg atcaaggcat cgctgcccaa cgtgcccgcg
    14701 agttgcccga tcagctgctc tcgcgtcact accccgccgt gctcgagact tgccgtatcc
    14761 aagtacagca acgtcaacgc ctgtccgacc aacatctccg caacggacag ttgacgtcgg
    14821 ctgatcttgt ccccggtggg cagcaagtag tagtagccat cgctcttgtg cacgagctcg
    14881 cactcgaagc ggcgatagag cggatcgagg tgctcggcgg catcggagag aaaagtgaac
    14941 caggcctcgt cgctgcgatc gatgtgccgc ccgcgtcgca acgccaaatc cacgtccggg
    15001 aaacactcgt gttggatggc gtcttccagc cgttcgaatc gagaagagtt catgggtcga
    15061 gctccggcca gtcttcgatg ctcacgccgc ccccgacgga gacccacggc cgttcgctca
    15121 cttcgcgagg ctgcgcaacc cgcgcaacgg tttcggcaat cttgccggcc gccaggaagc
    15181 gttggctcgg ggtctcgtct tccgtgagtt gagcggtgat ctcggcgagg ctgcgtttgc
    15241 cttcgcccag cgcgatcacc acgcgggcct gcaagtcggc gtcgggatcg ctcgcttcta
    15301 ccgtctccgc cgcggcctcg accggcttct tcttgcgtct cacgggcggg cgctcgatcg
    15361 gcggtagtac ctcacgcaga acccgcgcgg gcgccgcggc cgccacgcac agcgcgtagc
    15421 gccggccgcc cttaccggag agttgttcac gcaggcgctg gatcagcgtc cgcgaagggt
    15481 ccagacgcac cacgtctcgc aggtagcggt gcacgtactg gtagtactcg gaccacgcgc
    15541 gttgtcgggc cgagccccaa gccgcgatgc gatcgatctg atccatgatc gcgcgcagtg
    15601 ttcgatcggc ctcgtcgcgg ccggcgcggg cggcggcctc gtcgatgtct tgcaacaggg
    15661 tcatgatcgc gtgcgtgtcg cgcagcagca cctggttcag ctcgcccagt gtttgactcg
    15721 tggtgtcgag cagagtctgg cagcgctcca ccgcaccgaa ccaatccgcc cggacgagct
    15781 cgccgatttc gcgcttcaag cgttcctgtt cgagatcgaa gccgcgttgt cggcgttcga
    15841 tgccggaaag cagctcctgc acggtaatcg aaagcggcgc gaccacgagc gcctgaaaat
    15901 ccgagctctc gttcaagtcg gccgcaacgc gagcgacctc cgtgagactc gcgagcagcg
    15961 tgcgcgtgag cacggtcagg ctctcgcgcg tcagcgactc gtcttgcagg aagaactcga
    16021 cgatgccgag cgaaagccgg gtcagcgcgt actcgcccgc cctcgagagg cctgcgccgt
    16081 ccacgcgtgc gagtagcctt tgctcgcgcg cgcgtcgcag ggccgcgccg gcacgctttt
    16141 gcgcattgcc cgcgctcgga tcgaggacca acgcgacctg ctcgaaaacc tcgaacagct
    16201 gagcttcgtc gaaggcggag agttgagcgc gctccgcgcg caggtacagc gcggccagga
    16261 aacagatgtc cagtgtctcg agctccaacc tgacgttgcg ctcggcgagc gccaacaaag
    16321 cctgattggg ttccggagca gcgggtgaag acacgggcga aagcgggacc ggcttatacg
    16381 ccaccacccc gcggtctgca cgccaagcac gcgcgtcacg agcggttttg gccctgacaa
    16441 gccggccagt ggagcgatga cgcctcgctc gcgggcgcgc tccagaacgc ggcatcgtcg
    16501 cagagccgtc gtggagtcgt ggaaggtgcg ggcacctgcg cgttgtccac acgcgaacgc
    16561 ggcgaccgtg tagctccaac cagcgcgccg cgacctatct cgcgcgaaac accgggctgg
    16621 ctcgaaagca cgggcgtcag ggcttcagct ccaaaactcg cgaccgttca ccgaaccaag
    16681 gtcccatgtt cggacccccg gcacgagaaa cccaaaaatg aacgtgacca cgagaccaga
    16741 agacgcacag gccttggcgg aggccctgac cacacattgt tgcagcgaac tcttatccga
    16801 cttcggcttc gagccagtct ggttggcgcc agagcacgcc gttcccgatg gagtctcttt
    16861 ctgctccgta gtgggttttt cgggccagaa gctgcgcggc tcaatcatca tgtccagctc
    16921 ggggcgtgcc gcgaacgagc tctcttcgca tatccccggt ccgattcgcg acgtgatcgg
    16981 tgagatcgcg aatcagctcg gcggcaggct caagaacaag ttcttgctct atggagtcga
    17041 cctctacatg acgctgcccg tcgtcttgcg cggggagcac tttgcgccca tgcctcgcat
    17101 cgagctccga ccgatcgggt tctcgatcgg ttcgggatcg atgtgggtgt gggtcgatac
    17161 cgagtgtttc gacggcttcg aaatggcggt tcgcccgaac cccgccgaag cgccgatggc
    17221 agagggcgaa gcgctgttgt tctgaagttt ctgtcgcaat tcgccgttta ctggtgcgcg
    17281 cgcccggcaa gctttgcccc gcccaccgca gagatctttt ttcagtacgt gtgagtatca
    17341 ggagaacaag atgagtccga aggtgttgat tgtggatgat tctgcgaccg tgcggcaaca
    17401 agtgcggttg gcattgacgc aggctggctt cgacgtcatc gaagctaacg atggcctcga
    17461 aggcctcgac aagatcaaca gcgcttccga catcaaggcc gtgatttgcg acgttaacat
    17521 gcctcgcatg aacgggttgg agatgctgga gcgctgccat actcagggtg gtccccgcat
    17581 gccggtcttg atgctgacta ccgaaggcaa gccggcgctg attcagaagg ccaaagagta
    17641 cggagccaaa ggctggatcg tgaagccctt caaggcggac ctgctcgtgg ccgcggtgaa
    17701 gcaactcacg cgcgcagcct gaggcccgaa tgccgctccg gggcgtggct cgcgtgtttt
    17761 cgcgttggtt tgggcgtgga agcagacccg caagcgatac cgaggattcg gtaccgcccg
    17821 agacggatct gcgctccggc gtaatgcgct cggtgcctcc tttcctacct acgaacgatt
    17881 tggacggaag cgcgttcggc gtggaggcta cgcccggctt cgggatgggc gccgcccccg
    17941 tcggactgga cgcactcgcg gaaaacgagg ttccagttcc cgaggcaccg ccgccgagcg
    18001 agccgggcac gcccttgctc gaattggtgc gggagacatt cactcaatgt cgcaacaatg
    18061 tgctgtcacg gatccaatcc gtgaacgcga tgactgcacg cgaagtcatc gcagtcggtg
    18121 acagcctaca ggccatcgtc ggccgcgctc aagctcaggt cggtgcaacg cgcgacaccc
    18181 tgaaagggct gtccgggcag ggcagggccg gcgtcgccga gatcatcgag cgccagaccc
    18241 aaatggtgac cacgcacttg agcaccattc acgccgcgtt ggaacagcag accgagttga
    18301 cgcgccaagc tctggttgca tcctccggga tcgccgccat cggagagcag gtctccaagg
    18361 tggctctgca gacgcgattg ctatctctca atgccaacgt ggaggcagcg cgtctgggcg
    18421 ccaacggcgc tgggttcgag gtgattgcct cccaaatgcg ccgtctgacg gacgagatcg
    18481 atcaagctaa tcgtcagatc ggtcaaatgg ctcaggcgtt gctcgtgtcg ctgccccaaa
    18541 ttcaaaagca cggggagcgg ctgaaccgca gcgcggaggc gttctccaag gacttggccg
    18601 agaactcgag tcaggtctac ggcgcgaccg cccatctgaa gacatcgctg gaatccgtgc
    18661 tcgcacgcgg cgaccacgaa gcgggcttga ttctccaggg ctcgtacgaa gccatttcgc
    18721 acctgcaatt ccaggatcca acggctcaaa gcctgctcat catcgacgcg gatctcgcac
    18781 gcttgatgga gcgcgtgacg acgctactgg aagcctcccg tacgggctcg gtttcggtga
    18841 ccaatcactc gttcgaaaat ctcgaggcgg cagaatcgca cccggagtta cacgctggcg
    18901 aggtggtttc actcggttcg gaaccgttgg ccaccggcga cgtcatgctg ttctgagcga
    18961 actggttccc gtctgcatga cggctatcgt ccgaaccacg gcggcccgaa cgcggcatga
    19021 cgttcagggc ttcgcggcag tgcgcgtctg atctaaagtt aaaccggtcg acgtagacac
    19081 atgtggctgt aggcacatgg ttactgcgcg gcaagccggc tgtgcgcacg cgcgacagtc
    19141 atcgaaaaag gttggaagtc atgcccactg caagagagct gatgacaccc ggtcctgtta
    19201 ccgtgccggt cacggcgaag gtccagaaag cagtcgagat cctgcacacc gcggaggtgc
    19261 gccacctgcc cgtggtcgac gaagaaggca atctcgttgg catgctcagc gaccgtgatt
    19321 tgcgggcact ctctctgccc gtgttcgtcg gggacgaaca tgttgcgagt ctgcggacgg
    19381 cgctcaacgc gcctgtctcg acgctcatga actccgacgt cgtgtccgtc gaatccgagg
    19441 cggacgtgag tgaagtgatc gagctgatgc tcgacaacaa gatcggcgcg gtgccggtga
    19501 ccgacgcgga cgggctgctc acggggattg tcagctacgt cgatattctg cgcgcgctgc
    19561 cgttcgaaac agaaggcgac tgagcgctac ttctcgccgt ccgggtctgt aacgagagag
    19621 tgggacaaac gacacgaggc tcgctaaaca gcgagcctcg ttactgctac tcggtttggc
    19681 cgtgcgccgt cttcattcgg tcgctcggct acgtttggtg actcgactac ttggtgacta
    19741 cggccaggac ttctgcgccg gatcctcgcg caatgtccat gatgcggatc acttcgccgt
    19801 agggagcctc gtcttccgcc ttgaagaaca ccaccttttg gcggccaccg cgcaggcgcg
    19861 ccttgagctt ctcggtcagc tcctcgatgg agattggctc cgtattgacg ctcagctcgc
    19921 gtttcgccgt gtattccacg acgatcgtcg tatccgtaga cggtggagta ttatcctccg
    19981 ccttccgggg tacggtcgcc gtgagatgct tcaacatcga gggtgtcagc accatgaaga
    20041 tgatgagcaa caccagcacg acgtcgatga gcggcgtgac gttgatgtca ctgcgcggcc
    20101 ctttcttact gcctacatcc atcgacatgc tgtggcctcg cgactcttcc tatttcttca
    20161 atagattcgg ggaatgccgg ttctcgagcg ctcaatcagt tcttgagctc ttcggtacca
    20221 aggacgatgt cttcgatatc tgcgtcgtga agaacgtcca tggcttctcg aaccgtgccg
    20281 taggggacac gagtgtcccc tttgacgaag atggtctttt ctggcgtttt acggcgttcg
    20341 gcgattacgg cctggcccaa gtcctggacg cgattcacgc ggtttccacc gacgaagata
    20401 cgcttgtccg aggtaatcga gatgatgatg tctttgccgt cgtccggctt gcgggagtgg
    20461 aacttcgtgc gcggaagatc cacagccacg ccgctcgcga tcatgggggt gaccaccatg
    20521 aagatgatga gcagcaccag caccacgtcc accaacgggg tgacgttgat gtcggacatc
    20581 accttgcggg gctcacgctc gt
//
LOCUS       DASTKU010000442         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1869575, whole genome shotgun sequence.
ACCESSION   DASTKU010000442 DASTKU010000000
VERSION     DASTKU010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869575"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VFQ61_15165"
     CDS             complement(<1..540)
                     /locus_tag="VFQ61_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955849.1"
                     /translation="MERSSPLVHVLLVIYPMLRQCCFSLGKRTLNAWRGAILLSVLGV
                     SACRSQPEAGAPQRVGTPPSAARVIDSRDARNDPNAATDVRALSRPLNSPSPSGDPSE
                     ANSKEPRVGAESNSSEARVEVPPAKSSQPMPFLLILHGLGGSGEQMAQVLNLKTFAQE
                     KQFAYATPDGTPDSMGRRFW"
     gene            621..1415
                     /locus_tag="VFQ61_15170"
     CDS             621..1415
                     /locus_tag="VFQ61_15170"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9955850.1"
                     /translation="MMKMENRPLAVVTGASTGIGLELARCCAENGFNLLIAADEPEIY
                     QAAQSLGQTGVTVDAVEADLATQEGVDKLYAATRGQPVSALLANAGRGLGRAFLDQDF
                     TEVRRVIDTNVTGTLYLVQLIGRDMRARGEGRILITGSIAGFMPGTFQAAYNGTKAFL
                     DSFSFALRAELKDTGVTVTCLMPGATETDFFERADMLDTKVGAGKKQPADEVAKSGFE
                     AMMRGDGEVITGWQNKLRAAIAHLVPAGVAAEQHRGMAQPGSARNA"
     gene            complement(1450..2334)
                     /locus_tag="VFQ61_15175"
     CDS             complement(1450..2334)
                     /locus_tag="VFQ61_15175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HET9955851.1"
                     /translation="MFSRAPGLRRGLLLGSLTWVGLPWVGLSWVACQSGASCDAPDSQ
                     LAISTLRVCADPNNLPFSNAKGAGFENQLAELLGSYLGRKVEYTFWPSRRGFIRQTLK
                     RGLCDVVMGVPVGFELTENTAPYYHSSYVFVTRDDGSPAPVSFDDPRLLSWRIGLHTI
                     GDDYQNVPPAEALAGRGLISNIRGYPITGDYSQPDPPRRLLDALSDGEVDVAVVWGPL
                     AGFFAPLAGAKLRLSAIESRPDARANPELDHALPMTFGIAVGVRRGEHALRAELDHAL
                     AAERDRIRQLLDSYGVPR"
     gene            complement(2353..>3586)
                     /locus_tag="VFQ61_15180"
     CDS             complement(2353..>3586)
                     /locus_tag="VFQ61_15180"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9955852.1"
                     /translation="NKVLVGNSGGELGVRGWLLALDLDDGHEVWRAYHTGPDQDVRIG
                     PEFKPFYEKDRGPDLGVRSWPGEQWKLGGSTAWGWLSYDPELNLVYYGTGNPGVWNPD
                     LRPGDNKWSCAIFARNPDDGQAKWAYQISPHDAWDYDEIMENVLVDIEFKGRQRKLLI
                     HPGRSGFVFVMDRETGELLSAEPFQPVTWAKGFDLKTGRAKEDPDKRTHFGTITRDIC
                     PSSTGAKEFVPSAFSPRTGLLYIPAHNTCMDYGGIEANYIAGTPYLGADVKMFPGPGG
                     YQGELVAWDPIQGRRVWGVRESELPVYSGVLATGGDLVFYGTMDGGFKALDARTGREL
                     WKFKTGSGIVGNPITFLGPDGKQYVAIYSGVGGWMGATAFSNVSDDDPYAALGTVGAM
                     KSIKKYTGPGDMLYVFGL"
BASE COUNT          719 a         1116 c         1077 g          674 t
ORIGIN      
        1 ccaaaaccgc cgccccatgc tgtcgggagt gccgtccggc gtcgcataag cgaactgctt
       61 ctcttgggcg aacgttttca agttcagcac ctgcgccatt tgttctccgc tgccgcccag
      121 gccgtgcaga atcagcaaga agggcatggg ctgcgaggac ttcgcgggcg gaacctcgac
      181 gcgcgcctct gaagaattcg actcggcgcc cacccgaggt tctttcgagt tcgcttcgct
      241 cggatccccg cttggactcg gcgagttcag tggtctcgag agcgcgcgca cgtcggtcgc
      301 tgcattgggg tcgttccgag cgtcacggct atcgatgact cgtgcagcag acggaggtgt
      361 tccgacgcgt tgaggcgcgc ccgcttcagg ttggctgcgg cacgccgaca cacctagaac
      421 actcaataag atagcccccc tccaagcatt cagagttctc ttcccgagcg agaagcagca
      481 ttgtcgcagc atcgggtaaa tcacgagcaa aacatgcacc agcggcgagc ttcgttccat
      541 agctagccgc tcaccacggg ggatctcgct ttgggcacgc acgttgcagc cggaggctcg
      601 ctcctacgga ggcaactcac atgatgaaaa tggaaaatcg tcctttggcc gtcgttaccg
      661 gtgcgtccac gggaattggg ctcgaattgg ctcgttgctg tgcagagaac gggttcaatt
      721 tgctcatcgc ggcggatgag ccggaaatct atcaagctgc acaatccttg gggcaaactg
      781 gcgttaccgt agacgctgtc gaagcagatc tggcgacgca agagggggtc gacaagcttt
      841 acgcggcgac gcgtgggcag cccgtctcag ccctgcttgc caatgcgggt cgcggtctgg
      901 gtcgcgcatt tctcgatcaa gacttcaccg aggtgcgacg cgtaatcgat accaacgtca
      961 cgggtacact ctacctggtt caactcattg ggcgtgacat gcgcgcccgt ggtgaggggc
     1021 gcatcctaat cacaggctcg atcgcgggct tcatgcccgg taccttccag gctgcataca
     1081 atggcacgaa ggcgttcttg gattccttct ctttcgcgtt gcgcgcggaa ttgaaggaca
     1141 ccggcgtgac cgtcacctgc ctgatgcccg gcgcgacgga gacggacttc ttcgagcgcg
     1201 cggacatgct ggacaccaag gtcggcgcgg gcaaaaagca acccgcggac gaagtcgcca
     1261 agagcggctt cgaggccatg atgcgaggtg atggcgaggt catcacgggc tggcagaaca
     1321 agctgcgggc cgcgattgcg cacctcgttc ccgccggcgt cgccgccgag cagcaccgtg
     1381 gcatggcgca acctggatcc gcgcgcaacg cctagcgggg ccaatgacga gaaggccagc
     1441 ttcgcggcgt tatcgaggca ccccatagct gtcgagcaac tgccgaattc gatcgcgctc
     1501 ggctgcgaga gcgtgatcga gttcggcgcg cagggcatgc tcaccacggc gcactccgac
     1561 cgcgatcccg aacgtcatgg ggagagcatg atcgagttcg gggttggcgc gcgcatcggg
     1621 ccgggattca atggcgctta gtctcagttt ggcgcctgca agcggcgcga agaagcccgc
     1681 caacggcccc cagacgaccg cgacatcgac ttcaccgtct gacagtgcgt cgagcaagcg
     1741 ccgcggtgga tcgggctgag agtagtctcc ggtgattgga tagccgcgaa tgttggaaat
     1801 caagccgcgc ccggccagcg cctccgcggg cggaacattc tgataatcgt caccaatcgt
     1861 atgcagcccg atgcgccagc tcaataagcg tggatcgtcg aacgagacgg gcgcagggct
     1921 accatcgtcg cgggtcacga atacatagct cgaatggtag tagggcgcgg tgttctcggt
     1981 cagttcgaac ccgaccggaa cccccatgac cacgtcacac agaccgcgct ttagagtctg
     2041 ccgaatgaaa cctcgtcggc tgggccaaaa agtgtattcc actttgcgtc cgagataact
     2101 cccgagcaac tctgctagct ggttctcgaa acctgctcct ttcgcgttgg agaagggcaa
     2161 gttgttgggg tccgcacaca cgcgcaaggt ggatatggcg agttgcgaat ctggtgcgtc
     2221 gcaggaggcg ccgctctgac aagcaaccca ggatagacca acccaaggca gaccaaccca
     2281 cgtcagactg cccaacaaca gaccgcgtct caagccaggg gccctcgaaa acataggccg
     2341 gctcacggca catcagaggc cgaatacata cagcatgtcg ccaggcccgg tatacttctt
     2401 gatgctcttc atcgctccca ccgtgccgag cgcggcgtac gggtcgtcat cggacacatt
     2461 ggagaaagcg gtggcaccca tccagcctcc aacacccgag tagatcgcga catactgttt
     2521 gccgtccggg cccaaaaacg tgatcgggtt cccgacgatg cccgagcccg ttttgaactt
     2581 ccagagctcg cggcccgtgc gcgcatcgag ggctttgaac ccgccatcca tagtgccgta
     2641 aaacactagg tctcctccag tggctagcac tccactataa acgggcaact cactttcccg
     2701 cacgccccat acgcgccgtc cctgtatcgg atcccaagct acgagctcac cctgatagcc
     2761 gccgggcccc gggaacatct tcacgtctgc tcccaagtat ggtgtgcccg cgatatagtt
     2821 tgcttcgatt ccgccgtaat ccatgcacgt gttgtgagcg ggaatataaa gaagccccgt
     2881 gcgcggtgaa aacgcggatg gtacgaactc cttggctccg gtggaagaag ggcagatgtc
     2941 ccgcgtgatg gtgccgaagt gggttcgctt atcgggatcc tctttggcgc gccctgtctt
     3001 caagtcgaag cccttcgccc aggtgacggg ttgaaacggc tcggccgaga gcaactcgcc
     3061 ggtctcgcga tccatcacga acacgaagcc ggagcgcccc gggtgaatca gcagcttgcg
     3121 ttgccgaccc ttgaactcga tatcgacgag tacattctcc atgatctcgt cgtaatccca
     3181 ggcatcatgc ggcgagattt gataggccca tttggcttgt ccgtcgtccg ggttgcgggc
     3241 gaaaattgcg cagctccact tgttgtcgcc gggccggaga tcggggttcc aaacgccggg
     3301 gttgccggtg ccgtagtaca ccaggttcaa ctctggatcg tacgatagcc agccccaagc
     3361 ggtggagccg cccagcttcc attgctcacc gggccagctg cgcacaccta gatcgggccc
     3421 acgatccttc tcgtagaatg gcttgaattc aggcccaatg cgcacgtctt ggtccgggcc
     3481 agtgtggtac gcgcgccaaa cctcgtgacc gtcgtcgaga tcgagggcca agagccaacc
     3541 gcgcaccccg agttccccgc cggaattgcc gacgagtacc ttgttg
//
LOCUS       DASTKU010000443        14524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1646003, whole genome shotgun sequence.
ACCESSION   DASTKU010000443 DASTKU010000000
VERSION     DASTKU010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14524
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646003"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2384)
                     /locus_tag="VFQ61_15185"
     CDS             complement(<1..2384)
                     /locus_tag="VFQ61_15185"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9955853.1"
                     /translation="MSLSQPFIKRPVATSLVMVAILMFGILAYLGLPVAALPDVDFPT
                     IQVSAQLPGASAETMASSVATPLEREFSAIDGVASMSSENSLASTSITLQFDLKRDLD
                     SAAQDVQAAISRATPRLPRNMPTPPSYRKVNPADQPIVLLSLSSKTLPLYTVNEFAET
                     RLAQTISQVRGVAQVQVYGSQKYAVRVRLDPNKLASLNLGLDEVAAAIQEANVNVPNG
                     TLYGPRKAFAIDADGQLDNARAYEEVTLTYRNGAPVRVRDVGRALDSVENDKTAAWFG
                     APEGSQRAVILAVQKQPGANTVEVAQKISELLPQFRKEIPAAVSLDILFDRSLTVKES
                     VHDVQFTLLLTLALVVGVIFLFLRRLTATVIPSLSMPLAILATFAGMHLLGYSLNNLS
                     LMAITLSVGFVVDDAIVVLENIVRHIEMGKPPLQAAYDASAEIGFTIVSMTLSLAAVF
                     IPFLFMGGILGSLFKEFAVTIALSILVSGFVSLTLTPMLSARMLSAEQGHSETQAKRP
                     GWFMRFVEWVLVGMLRFYEKTLNVVLRHPRATLAFSALVLAGTIALFVVIPKGFLPTE
                     DTDQLSVTTEAVEGMSFEAVAERQQQIAEIVRRNPDVAGFMSNAGARGRSGTNQGNLF
                     IRLKPRAERAHGAAEVARVLKGQLAEVPGMRSFVQVPPPIRLGGRVTKSEYQLALQGT
                     DTALLYKEALRLTGELAKSPILTDVTSDVQLNNPEIAVRIDRERAARLGVSAARIEDA
                     LYSAYGSRQVSTIYAATNSYAVILELEPSSAREPTALEKLYVRSNSGALVPLPSV"
     gene            complement(2381..3526)
                     /locus_tag="VFQ61_15190"
     CDS             complement(2381..3526)
                     /locus_tag="VFQ61_15190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9955854.1"
                     /translation="MKSAKSASPTRWIGLICVLMGLGTAACSKKSAGAQPEREQVTVN
                     VALVERRDVPLELQTIGTVEASSTVEVLPQVTGLVTEVHFKEGDFVKKGDKLFTIDTR
                     PYRASLAVAQAELARSRAQSAQAQSEADRYAKLHAEGLATEQEMMKAQADAASSRAAV
                     QLGEAQSRSANLNVQFAHITSPIEGKAGSLLVYPGNVVSAGARQPLVVIRRMAPVYVR
                     FAVPEQYLPKIRERMGKEKIAVRAKPRGGTSAAEGELTFLENTVDSATGTLALKATFA
                     NANSELWPGASVEVTLTLDVEAGVTVAPEAAVLEGQNGHYAFAIERGNRAALRPVTLI
                     RTTDKLALIRSGLSPGEQVVTDGQVRLRNGMVVKVKPATTETGERAQ"
     gene            3628..4332
                     /locus_tag="VFQ61_15195"
     CDS             3628..4332
                     /locus_tag="VFQ61_15195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9955855.1"
                     /translation="MDRARLSVLLVEDDAKLARLTRDYLEQHDVGVIWVSDGDSAVRE
                     ALRPAIDAVILDLMLPGRDGFEVCREIRAHSHVPIIAVTARVEVADRVLGLELGADDY
                     VTKPFSARELLARVRAVVRRAEGRSGPTARELTAGSLVLDLARLSATLDGRPLALTSY
                     EFSLLRALVENAGRVLSRERLLELAKGNADEAFDRSIDVRISRLRQKLGDDSKHPVLL
                     KTVRGSGYVLTAPEPK"
     gene            4329..5675
                     /locus_tag="VFQ61_15200"
     CDS             4329..5675
                     /locus_tag="VFQ61_15200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9955856.1"
                     /translation="MKASLLLRVYLHGILMLALATGASFVVGTYVLKPALEVPARPST
                     TWIAWHLAALVDQPEQLRAELEDVRRVRVEVSLFEPSGKLIATNAKRVPPPLSARELR
                     ALPQEPTRFAGGTGVVATFDHGTLLRYARVSYPAPELPLRIALGQLAVALLVIAALSM
                     PLARSISAPVERLAALARALGAGNLAARARSRRRDEIGDLARAFDEMAERLVQLRRSE
                     KELLANVSHELRTPLARIRLALELVRDGDTRRADSYLTDIEEDLAELEQLLDDVMTAT
                     RLELARGGDALPPLRRQRVAALDVLEAAKLRFARRFPERRLESRFQANLPHLDADPTL
                     LRRVLDNLLDNAVKFSDAAAPIELEAERDATGAELVLRVRDQGVGIPAADLERVFEPF
                     FRSDPSRTRSTGGVGLGLTVVRRILHAHGGSIRVESTPQQGTRFEIRVPEAPPPAD"
     gene            complement(5685..6971)
                     /locus_tag="VFQ61_15205"
     CDS             complement(5685..6971)
                     /locus_tag="VFQ61_15205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9955857.1"
                     /translation="MSRLRIPGLRWWIIGLVMLGSSINFLTRSTLAVAAPTLLGELNI
                     SSQQYSWILAAFQLAIMTQPVCGYVLDVIGLRVGYTVFALAWSVVNMLHGFANGWPVL
                     AGLRALLGFAEGSSNPAGMKATAEWFPAKERGLAGGIYNIGASVGSMLAPPLVAWAVL
                     TYNWRASFWITGALGVAWAVLWAAVYRSPERHPALGADEKRYITAGQEQHLESDGARP
                     PVLGILKQRNFWGIALPRFLADPTWGTLTFWLPLYLHDVRQFDLKQIALFAWMPFLAA
                     DVGCVFGGVLSMRLARSGMRLVDARRWSFTLGAILMLGVTGVGYVESPHWAIALLSLG
                     GFAHQTLSVTVITMASDLFKRNQVATVTGMSGTLGNAGVLLFSLLIGARVKEVGYTPF
                     FVGLGLLDLLGAALLWILVRERSSGRTAEQVAELRS"
     gene            complement(7299..7805)
                     /locus_tag="VFQ61_15210"
     CDS             complement(7299..7805)
                     /locus_tag="VFQ61_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955858.1"
                     /translation="MTRKKESVVAFQVMSEAFVTSGNSMGAPIGSYRANGISTSLVAP
                     PAGDAPPSPVGGAPPSFGGKVELVEAPVLEAVDEVEPPCVAFDRPPVAFPPVFEDDPV
                     FEDDPVFEGDPVPPDAPAPADPPVEWAPEALVGGDSTESFSEQPSGKTLAVLQRRAAR
                     AWPRVRCC"
     gene            7804..8505
                     /locus_tag="VFQ61_15215"
     CDS             7804..8505
                     /locus_tag="VFQ61_15215"
                     /inference="COORDINATES: protein motif:HMM:NF018659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HET9955859.1"
                     /translation="MDRVAEFREYPPDFGSPRRIIRIMNGPVDAEGLTYIGSHAGLDR
                     FALSIEASQDLVMIFDLAPDATSIDMRTAVLQTYEPAAAPAIANKGWEGVAFRPAHGG
                     SAPRLYACQEGEPGQAPTRVLSFPYLPEAGTTLSYQNGSLSVDEPWNALDRLGSVAGD
                     LSSVHYDQLTDTLLLLSHLGSRLLRVEPESGEIMDQLALNRSPQYEGVTLTSDGRLVL
                     VSEPNFVEIFQRPDP"
     gene            complement(8534..10042)
                     /locus_tag="VFQ61_15220"
     CDS             complement(8534..10042)
                     /locus_tag="VFQ61_15220"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9955860.1"
                     /translation="MGYNTRLGGVFAFCGSCLIGCSSGGFETRTDEQQTDESEIATRD
                     SAIVRATSAGGRNEVVMLYVTVSNAAGLSTRICSGTYFASRVVLTAAHCLQDALLNQV
                     FIYYGDNFAQDFSQLVASGSLLEPPAVGQPSSWAQADSFELHPQWDSTLLYPDLAVVY
                     LDRKLPFEPLPVARFRLDNSSLNKEATICGWGADQASTPTTGSGLHVQRTGKTHILGS
                     PTQADYHPEDPNPGMLQASVRQNVIKTDGHAPNSNTCFGDSGGPLIFNKSGQDYVAGV
                     SYFTGLSCEDYSLFVRLDPFLPFIDRATKRGGQELLLPTFDCVTQTTTGYRAFFGYNN
                     KNGVSLSIPYGIKNLLARDTAGLRPTLFMPGEHHFAFAVDFAANQNVSWMLIPDNNSV
                     VALSIGANSKKCGAADAQAVECAGFCSAAHNSGCTNPPPFEACESECLTAASFFQDVF
                     PQCMPLNSALNQCFARVPGGLSHWTCLDGATPQPVTECQAEAAALYQCIGLQ"
     gene            complement(11024..13108)
                     /locus_tag="VFQ61_15225"
     CDS             complement(11024..13108)
                     /locus_tag="VFQ61_15225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9955861.1"
                     /translation="MPVKSRTGGATGRCIIRVIALSSDRPSPELVSPELIELRQRIRE
                     LESEVARLRTRRLSQEEAQSGLAEALTERLAAYEEVERLAKVGSWFWDLRTNEVKWSA
                     ELYRILGYPADQAASVELFFAALHPEDRERVRARSQVTSSTGLTEPGVSCRVLQPDGS
                     LRHIKLIGSAILDAEGQLVRLVGAVLDVTAFVLNEEELRRQARLLDEAHRVAGISSWI
                     WRREQGRFEWSETSTPLIGVESGAGDSSELPKFVHPEDLAAWEAARAQIARGVAPPPL
                     TFRIVRPDGATRYMTMDAKLDESGTVLGTLQDVTGRWELEQRLRHSQKMEAVGTLAGG
                     IAHDFNNYLVVIQGNLDLLRSSEQSASSSQSFDEMARAAERCASLTRQLLAFARRHPD
                     AAQLVDFEQIIAGSAGMLKRLLGPGIELIWSTESTDLRVLADPSQIELVIVNLAVNAR
                     DAMPSGGKLFIDVAPKHLSNREPNGEIGAGEYVVLRVADTGTGIPEGVRERLFEPYFT
                     TKPPGHGTGLGLATVYGIVKQSRGHIRIESRLGHGSTFTLYFPARRGVQGSPHVPKPS
                     TTLAQGETILVVEDEPSVRRLTRRLLERGGYKVLEAADGLQALELASRSQPLDLVLTD
                     INMPRMDGIALSRRLRAADPHVKLLMMTGNPSFSLQELTRDLEDCPILSKPFDLERLL
                     GAVRAALGQGAR"
     gene            complement(13244..13906)
                     /locus_tag="VFQ61_15230"
     CDS             complement(13244..13906)
                     /locus_tag="VFQ61_15230"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET9955862.1"
                     /translation="MFAKTAIRTSVLLAMGLAASCSNDEDASSVVQWGGGGSGAVAGA
                     SAGGRSGAGNSQGGNAGASEGGSSTSPSNLSDAQVFGVLDTANAGEIEQGNLALEQAS
                     NADVQAFAQQMVTDHTAARAEARALAEAQSTSIQESSVSRGLQQKAASKTAELSALSG
                     AAFDSAYISVQIEMHTETLLLIDQALTLTTDNEELNSLLEKLRPKVQAHLDHAQSIAV
                     GL"
BASE COUNT         2746 a         4802 c         4350 g         2626 t
ORIGIN      
        1 accgacggga gtggcaccaa agccccacta ttcgagcgca catagagctt ctcgagcgcg
       61 gtgggctcgc gcgcactgga gggttccagc tccaagatta cggcgtagct attggtggcg
      121 gcataaatcg tggagacttg ccgggagccg taggcgctat acagcgcgtc ctcgatgcgt
      181 gccgcagata cgccgagtcg cgcggctcgt tcgcggtcga ttcgcaccgc gatctctgga
      241 ttgttgagct gcacgtcgct cgtcacgtcc gtcagaatcg gcgacttggc gagctcaccc
      301 gtgagacgaa gcgcctcctt gtaaagcagc gccgtgtccg ttccctgcag cgccagctgg
      361 tactcgctct tggtcacccg ccccccaagc cggattggcg gcggaacttg aacgaaagag
      421 cgcatgccgg ggacttcagc cagttgcccc ttgagcacgc gcgccacctc cgccgcgccg
      481 tgggcgcgct cggcgcgcgg cttcaagcga atgaacaagt ttccctggtt cgtgccgctg
      541 cgaccacgtg cgcccgcgtt cgacatgaag ccggccacat ccgggttccg cctcacgatc
      601 tccgcgattt gctgctggcg ctccgccacc gcctcgaacg acatgccctc gacggcctcg
      661 gtggtcaccg acaactggtc ggtatcctcc gtgggcaaga acccctttgg gatgaccacg
      721 aacagcgcga tcgtgccggc cagcaccaaa gccgagaagg ctagtgtggc gcgcggatgg
      781 cgcagcacca cgttcagtgt cttctcgtaa aagcgcagca tgccgaccaa cacccactct
      841 acgaagcgca tgaaccagcc cgggcgcttc gcctgcgtct cgctgtgtcc ttgctccgcg
      901 ctcagcatcc gcgcggagag catcggcgtc agagtcaacg agacgaagcc ggagaccaag
      961 atcgagagcg caatcgtaac cgcaaactcc ttgaacagac tgcccaggat gcctcccatg
     1021 aacaagaagg ggatgaacac cgccgccagc gagagggtca tcgaaacgat ggtgaagccg
     1081 atttcagccg aggcatcgta ggccgcttgg agcggaggct tacccatctc aatgtgccgc
     1141 acgatatttt ccagcacgac gatcgcgtcg tccacgacga agcccacgct caacgtgatc
     1201 gccatcaaag acaggttgtt cagcgaatag ccgagcaggt gcatgcccgc gaaggtcgcc
     1261 aaaatcgcga gcggcatgga caaactcgga attaccgtcg ccgtgagccg ccgcaagaac
     1321 agaaatatca ctccaaccac gagcgccaac gtgagcaaca acgtgaactg cacgtcgtgc
     1381 acggactctt tcaccgtcag cgagcggtcg aataggatat cgaggctcac ggccgctggg
     1441 atctctttac gaaactgagg caagagctcg gaaatcttct gcgcgacctc caccgtgttg
     1501 gcgccgggtt gcttttggac ggcgagaatc acggcgcgct gactgccttc gggagcgcca
     1561 aaccaagccg cggtcttgtc gttctccaca ctgtccaaag cccgcccaac gtcccgcacg
     1621 cgtacaggag cgccgttgcg ataggtgagc gtgacctcct cgtaagcgcg agcgttgtcg
     1681 agctggccgt ccgcgtcgat cgcgaaggct ttgcgcggac cgtatagcgt gccgttcgga
     1741 acgttgacgt tcgcctcctg aatggcggcc gccacctcgt ccaaaccgag gttcaagctc
     1801 gccagcttgt tgggatcgag gcgcacgcgg acggcgtact tttgagagcc atacacttgc
     1861 acctgggcca cgccgcgaac ctgcgaaatg gtctgggcga gccgcgtctc ggcgaactcg
     1921 ttgacggtat agagaggcag cgtcttggag ctcaaggaca gcagtacaat cggctgatct
     1981 gctgggttta ccttgcgata gctcggcggc gtgggcatgt tgcgcggcaa gcgcggcgta
     2041 gcccgcgaga tcgcggcttg aacatcctga gctgccgagt ccaaatcgcg cttcaggtcg
     2101 aactgcaggg tgatgctggt cgaggccagc gagttctcgg aggacatcga ggccacgccg
     2161 tcgatcgccg agaactcgcg ctcgagcggg gtcgcgaccg atgacgccat cgtctccgcg
     2221 ctcgcgccgg gcagttgcgc ggagacttgg atggtcggaa agtctacgtc cggcagggct
     2281 gcgaccggca ggcccaggta cgccagtatg ccgaacatca agatcgccac catgaccaag
     2341 ctggtagcaa cgggccgctt gatgaacggt tgcgacaggc tcattgggcg cgctccccgg
     2401 tctcggtcgt cgccggcttg accttcacga ccatgccgtt gcgcagtcgg acctggccgt
     2461 cggtcacgac ttgctcaccg gggctcagac cgctccgaat caaggccagc ttgtcggtgg
     2521 tgcgaatcaa ggtcacgggc cgtagcgccg cacgattacc gcgctcgatc gcaaacgcat
     2581 aatgcccgtt ctgtccctcc aataccgccg cctccggagc cacggtgacg ccggcctcca
     2641 cgtccaacgt gagcgtgacc tcgacgctcg cgcccggcca aagctcggag ttggcattgg
     2701 caaacgtggc cttgagcgcc aacgttcccg tcgccgagtc caccgtattt tccaagaacg
     2761 tgagctcacc ttccgccgcg ctggtcccgc cccggggctt ggcgcgcacc gcaattttct
     2821 ctttccccat gcgctccctg attttcggga ggtattgctc gggcaccgcg aatcgtacgt
     2881 agactggcgc catgcgtcga atcacgacca gcggttgacg cgctccggcc gagacgacgt
     2941 tgccgggata gactaagagg ctgcccgctt tgccctcgat gggcgaggtg atgtgtgcaa
     3001 actgcacatt caagttggcg ctgcggctct gcgcttcgcc gagctgcacg gcggcgcggc
     3061 tcgatgccgc atccgcctgc gccttcatca tctcctgttc cgtggcgagc ccttcagcgt
     3121 gcagcttggc gtagcgatcg gcctcgcttt gcgcttgcgc cgactgcgcg cggctgcgcg
     3181 ccaactccgc ctgcgcgacc gccaaggagg cgcggtaggg gcgcgtgtcg atcgtgaaga
     3241 gcttgtctcc cttcttcacg aagtccccct ctttgaagtg gacctcggtc acgagccccg
     3301 tgacctgcgg caagacctcg acggtactgc tggcctctac cgttccgatc gtctgcagct
     3361 ccaacggcac gtctcgtcgc tccacgagcg ccacgttcac cgtaacctgc tcacgctcgg
     3421 gttgggcgcc cgcgctcttt ttcgaacacg ccgcggtacc cagacccatc agcacacaaa
     3481 tcagtccaat ccaacgggtc ggggaggcag acttcgcaga cttcacttgc ataacgtggc
     3541 gcccgagcct ttcgggactt tctccgaatt gtgtaggaat gtgtcggccg ctgggcggca
     3601 gggcggcatg gtgtagtttg tgcaccgatg gatcgggcca ggctcagcgt attgctcgtc
     3661 gaggacgatg ccaagctcgc gcggctgacg cgcgattacc tcgaacagca cgacgtcggc
     3721 gtgatctggg tttcggacgg ggattccgcc gtgcgcgagg cgctgcgtcc cgccatcgac
     3781 gccgtgattc tggatttgat gttgccgggg cgggatggct tcgaagtctg tcgagaaatc
     3841 cgcgcgcatt cccacgttcc gatcattgcc gtcaccgcgc gcgtcgaggt cgccgatcgt
     3901 gtcttgggct tggagctcgg tgcggacgac tacgtgacca agccattctc ggcgcgcgag
     3961 ctcctggcac gcgtgcgcgc ggtggtgcgt cgcgcggagg gccgctccgg gcctacggcg
     4021 cgcgagctca cggcgggcag cctggtgctc gacctcgcgc gcttgtctgc gaccctggat
     4081 gggcgcccgc tcgcgctcac ctcctacgag ttctcgctac tgcgcgcgtt ggtcgagaac
     4141 gccggcagag tgctgagccg cgagcgtctg ctggagctcg cgaagggtaa cgccgacgaa
     4201 gcgttcgatc gttcaatcga cgtgcgcatc tctcgcttgc gccaaaagct cggagacgac
     4261 tcgaagcacc cggtgctgct caaaacggtc cgaggctccg ggtacgtgct caccgcgccc
     4321 gaacccaaat gaaggccagc ttgctgctgc gggtttacct gcacggaatc ttgatgttgg
     4381 cgctcgccac cggcgcaagc ttcgtagttg gcacgtacgt gctgaagccc gcactggagg
     4441 tccccgcccg accgagcacc acctggatcg cctggcacct ggcggctttg gtcgatcagc
     4501 ccgaacagct ccgcgcggag ctcgaagatg tccgccgcgt gcgcgtcgaa gtctcgctgt
     4561 tcgagcccag cggcaagctg atcgcgacca acgccaagcg cgtgcctccc ccgctctcgg
     4621 cgcgcgagct gcgcgccttg ccccaagaac ccacacgctt tgcgggcggc accggggtgg
     4681 tcgccacgtt cgaccacggc acgttgctcc gttacgcgcg cgtgagctac cccgcacccg
     4741 agctccctct ccgcatcgcc ctcgggcaac tggcggtcgc gctgctcgtg atcgccgcgc
     4801 tatcgatgcc cctcgcgcgc tcgatttcgg cccccgtgga gcggctcgcg gcgttggcgc
     4861 gcgccttggg cgccggcaac ctggcggccc gcgcgcgtag ccgacggcgt gacgaaatcg
     4921 gggatctcgc gcgcgccttc gacgagatgg ccgagcgcct cgtgcagctg cgtcgctccg
     4981 aaaaagagct gctcgccaac gtgtctcacg aactgcgtac gccgctcgcg cggatccgcc
     5041 tggccctgga gctcgtgcga gatggggata cgcgccgtgc cgacagctac ctgacggaca
     5101 tcgaagagga tctcgcggag ctcgagcagc tgctcgatga tgtgatgacc gccacacgac
     5161 tcgagctcgc ccgcggcggg gacgcgcttc ccccgctgcg tcgccaacgg gtcgcggccc
     5221 tcgacgtctt ggaagctgcg aagctgcgct tcgcgcggcg tttcccagag cgacgcctcg
     5281 agtctcggtt tcaggcgaac ctaccgcatc tggacgccga tccgacgctg ctgcgtcgcg
     5341 tgctggacaa cctgctcgac aacgcggtca agttctcgga cgcggcggcc ccgatcgaac
     5401 tggaggctga acgagacgcg accggggccg agctcgtgct gcgggtccga gaccaaggag
     5461 ttggtatccc ggccgctgat ctggagcgcg ttttcgagcc gttcttccgc tccgatccca
     5521 gccgcacacg cagcacgggc ggcgttggct tggggctaac cgtagtgcgc cgcatcttgc
     5581 acgcccacgg gggcagcatt cgcgtcgaga gcactccgca gcaaggcacc cgcttcgaaa
     5641 ttcgagtccc cgaagcgccg ccgccagccg actgaggctg gaaatcagga acggagctcg
     5701 gccacctgct ccgccgtcct cccgctgctc cgctcgcgca ccaaaatcca aagcagcgcc
     5761 gcgcccaaca gatctaagag cccgagcccc acgaagaacg gcgtgtaccc cacctccttc
     5821 actcgcgccc caatcaacag cgagaagagc aagactccgg cgtttccgag cgtgccactc
     5881 atcccggtca cagttgcaac ttgatttcgt ttaaacaaat ccgacgccat cgtgatgacc
     5941 gtgaccgaga gcgtctggtg ggcgaaccca cccaagctca acagcgcgat cgcccaatgg
     6001 gggctctcga cgtagcccac ccccgtcacg cccagcatga gtatcgcgcc cagcgtgaac
     6061 gaccaacgcc gcgcgtccac cagccgcatt cccgaccgcg ctagcctcat actcagcact
     6121 cccccgaaca cgcaccccac atccgcggcc aaaaacggca tccatgcgaa caaggcgatc
     6181 tgcttgagat cgaactggcg cacatcgtgg agatacaacg gtaaccagaa cgtcagcgtg
     6241 ccccaagtcg gatccgccag aaaacgcggg agcgcgatgc cccagaagtt ccgctgcttc
     6301 agaataccga gcaccggcgg ccgcgcgcca tcactctcca ggtgctgctc ttgacccgcc
     6361 gtgatgtagc gcttctcatc ggcgccaagc gccgggtgtc gttccggcga gcgatacacc
     6421 gccgcccaca gcacggccca cgctaccccg agcgcgcccg tgatccagaa tgaagcgcgc
     6481 cagttgtacg tcagcaccgc ccacgccacg agtggcggcg ccagcatgga acccaccgaa
     6541 gccccgatgt tgtagatccc gcccgcgagc cctctctcct tcgcggggaa ccactcggcg
     6601 gtcgccttca taccggcggg gttggaggaa ccctcggcaa accccagcag cgcacgcagg
     6661 cccgccaaca cgggccaacc gttcgcgaac ccgtgcagca tattgaccac cgaccaggcg
     6721 agcgcgaata ccgtgtatcc aacgcgcagt ccaatcacgt ccaagacata gccacagacc
     6781 ggttgggtca tgatcgcgag ctggaacgcc gccaaaatcc aggaatattg ctgcgaactg
     6841 atattcaatt cgcccagcaa cgtgggagcc gcgaccgcca gcgtactgcg cgtcaggaag
     6901 ttgatgctcg accccagcat cacgagccca atgatccacc acctgagccc tgggatacga
     6961 agtcgcgaca tggtttccgc catggggcgg cggagcctcg tccgttcatg ctgggatgtc
     7021 aattggacct ggaggcgaga aatccgggtt gaatggccgc agtgtcttcg ggcgcgccgt
     7081 gcgtctgctg aatccggatg cgcagcccct gcccccttgc ctcccctgac accgcgccgg
     7141 cccccccaat cacgaccaca ggattagagc ccgatgatcg cggcaacgta gcgggcgcgt
     7201 ccggacgccg ccctctgcac tagatctcgc tcacgatccg gatcacgatc cgggctacaa
     7261 gaggaacgga tgacccactc cgctcgacca atccgcgatc aacagcagcg cactcgaggc
     7321 caagccctcg cagcccttct ctgcagtacg gcaagcgtct ttccgctcgg ttgctcggag
     7381 aaggactctg tggagtcacc cccgaccaac gcctcagggg cccactccac gggcgggtcg
     7441 gcgggcgctg gcgcgtctgg gggcactggg tcgccctcga acactgggtc gtcctcgaac
     7501 actgggtcgt cctcgaacac tggcgggaac gcgaccggtg gtcggtcgaa tgcgacgcaa
     7561 ggtggttcta cctcgtcgac agcctccagc accggagcct cgactaactc cactttgccc
     7621 ccgaatgagg gaggtgcccc gccgaccggc gaaggcggag cgtcacccgc cggaggtgca
     7681 acaagcgaag tcgatatccc gtttgctcga tacgagccga tcggcgcgcc catcgagttg
     7741 ccggacgtca cgaatgcctc ggacatcacc tggaaggcca ccactgattc cttcttccta
     7801 gtcatggacc gagtggcgga gtttagggag tatccgccgg atttcggttc gccacgccgc
     7861 atcatccgaa tcatgaatgg cccggtggac gcagagggcc tcacctatat cggctcccac
     7921 gcaggtctgg atcgcttcgc tttgtcgatc gaggcgagtc aggacttggt gatgatcttc
     7981 gatctcgcgc ccgacgcgac atcgattgat atgcgcaccg ccgtactgca aacctacgaa
     8041 cctgcagcgg cccccgccat tgcaaacaag ggttgggaag gtgtggcatt tcgtccagca
     8101 cacgggggca gtgcaccgcg cctgtatgct tgccaggaag gagaaccggg ccaagcgcca
     8161 actcgcgtgc tgagttttcc atatctgcca gaggcgggca ccaccctcag ctaccagaat
     8221 ggctcattga gcgtggacga accctggaac gctctcgatc gtttaggcag cgtggccggt
     8281 gatctctcga gtgtgcacta cgaccagctg acggacacgc tacttctgtt atcgcatttg
     8341 gggagtcggc tgctccgggt cgaacccgag tcgggcgaaa tcatggacca actggctttg
     8401 aatcgctcgc cgcaatacga gggcgttacg ctgacgagcg acggtcggtt ggtgctcgtc
     8461 agcgaaccca atttcgtcga gatctttcag cgtcccgacc cgtaagctag gcctctttca
     8521 ctgcagacct ctttcactgc agaccaatgc actgatacaa cgccgcggcc tcggcctgac
     8581 actcggtgac aggttgcggc gtcgctccgt cgagacaagt ccaatggctg agccccccag
     8641 gcactctcgc gaagcactgg tttagagcgc tgttgagagg catgcactga gggaacacgt
     8701 cttgaaagaa acttgccgcc gtcaggcact cgctctcgca ggcttcgaag gggggcgggt
     8761 tcgtgcaacc tgaattgtga gccgcgctgc agaagcccgc gcattccacg gcttgggcgt
     8821 ccgccgcgcc acactttttc gagttcgcac cgatggacaa tgcaactacg gagttgttgt
     8881 cggggatcag catccagctc acgttttgat tcgccgcgaa atctaccgcg aatgcgaagt
     8941 gatgttcgcc cggcatgaac agcgtgggcc gcagacccgc agtatctcga gcgagtaagt
     9001 tcttgatccc gtaggggatg gagagcgaga caccgttctt gttgttgtat ccaaagaagg
     9061 cgcggtagcc ggtcgttgtc tgcgtgacgc agtcgaacgt gggcagaagc agctcctgcc
     9121 caccccgctt cgtcgcgcga tcgatgaatg gcaagaacgg atcgagacgc acgaacaagc
     9181 tgtagtcctc gcaggacaaa cccgtgaagt agctcacccc ggcgacgtaa tcttgccctg
     9241 acttgttgaa gatcagcggc ccccccgaat cgccgaaaca agtattggaa ttgggcgcgt
     9301 gcccgtctgt cttgattacg ttttgccgca cgctggcttg cagcattccc gggttcggat
     9361 cctccgggtg gtagtcggcc tgcgtcggcg aacctagaat gtgggtcttg cccgtgcgct
     9421 gcacatgtag gccgctgccc gtcgtcgggg tagaggcttg atccgcaccc cagccacaaa
     9481 tcgtggcctc tttgttcaat gaggaattgt cgagccggaa ccgagccacc ggcaacggct
     9541 cgaatggcaa ctttcgatcc aagtagacca ctgccaaatc cggatacaga agcgtagaat
     9601 cccactgtgg gtggagctcg aaagaatcgg cctgcgccca actcgagggt tgccccaccg
     9661 cgggcggttc gagcaacgag cccgaggcca ccaattggct gaaatcctgc gcgaaattgt
     9721 ctccatagta gatgaagacc tggttcagaa gggcatcctg caagcagtga gcagccgtca
     9781 gcaccacacg cgacgcaaag tacgtaccgg agcagatacg tgtgctcagc cccgctgcgt
     9841 tcgagacggt aacgtacagc atgacgactt cattgcgccc ccccgccgag gtggcacgca
     9901 caattgcgct gtcccgagtc gcgatctccg actcgtcggt ttgctgctcg tctgtccgag
     9961 tttcgaatcc tcccgaggag caaccgataa ggcagcttcc gcaaaaggca aacacaccac
    10021 ccagtctcgt attgtatccc atcgagcact cctgtatcgc cgaccaccac caccaccgcc
    10081 gaactcgcgg catgagctcg gaaagcgccg gcctgaccgt tgctaacgcg cgaacttcac
    10141 gaggccgccc gaaagtccca gtatttgctc aaaccagcat cgcctgaaac cagtatcgcc
    10201 gaaacctcta tcgccgaaac ctggtgaggc ttggcgatca aacccaagaa ggcatgctca
    10261 cccacgaacg atgtcaaact gggcttgcgg gcgttcccca cgggagttcg cagtaccctt
    10321 aataccatag gaataggccg acagtctttt gatactccac tcagcgtcga tactccactc
    10381 tgctttgcaa ctgactctgt tttttgggac ccggctctgc ttcgctattg cccactctgc
    10441 tctaatagtc catcgagctt actcagctgc gcggagtgaa agtactttcg gatagcactc
    10501 tgctatccga agctcaaagc agagtttcgg ccgctcggcg tgaagaacgg gtgttttggc
    10561 ccgtcaccct tcgccatgcg tttgacgcaa gaccctagac gcaagctggt cgcacctcgg
    10621 agtctagagc gtcccgtcag cctcgtgtgc gccaagttgg aattccacct tctggcggaa
    10681 cccagcgggt atagctgatg caactcaaca ctaatcccct atcggctcgt tctatagtca
    10741 cgccctatta tccagcgttt ccctggcatc gttcctccag gtcgaagatg ccgactcgag
    10801 cgatcaacga gaactcgggc gtacaatgga gtcagcgaag cctgccctgc gaatgtcgag
    10861 agagtcgcgt attcggggac gcatagtaaa tcgctcgaca gagcgctgtc gaacccgagc
    10921 tagctcgaat cgccatgcgc ccggagcaac gccggagcct cgccacggtg catcgagagc
    10981 ttgccccccg actcgcgagg ccgcacgtaa acccacttct cagctacctg gcgccctgcc
    11041 ccaatgcggc acgcacagcg cccaatagac gctcgagatc gaacggcttg gacaagatgg
    11101 gacagtcttc caaatcgcgg gtcagctcct gcaagctgaa gctcggattc ccggtcatca
    11161 tcagaagctt cacgtgagga tccgccgcgc gtagccggcg cgacagggcg attccgtcca
    11221 ttcgcggcat attgatgtca gtcagaacca gatccagggg ctgggagcgt gaagccagtt
    11281 ccaaggcctg gagcccgtcc gcggcttcca ataccttgta tccgcctcgt tcgagcagcc
    11341 gtcgggtgag acggcgcacg ctcggctcgt cttccacgac taagatggtt tctccctgcg
    11401 ccagcgttgt cgacggcttg ggtacgtgtg gcgagccctg tacgcctctt cgcgccggaa
    11461 agtagagggt gaaggtggag ccgtgcccga gacggctctc aatccgaatg tgccctcggg
    11521 attgctttac aattccgtac acggtcgcca agccaagacc cgtgccatgc cctggcggct
    11581 tggtggtgaa gtacggttcg aataggcgtt cccgcacacc ctccggtatt ccggttcccg
    11641 tgtccgcgac cctaagcacg acgtactcgc cggcgccaat ctcgccattc ggttcgcgat
    11701 tgctcagatg cttcggtgcg acatcgatga ataacttgcc gccgcttggc atcgcgtcgc
    11761 gggcgttcac cgccaagttc acgatcacga gctcgatttg cgaagggtcc gcgagcaccc
    11821 gcaagtccgt gctctccgtg ctccaaatca gctcgattcc tggtcctagc agacgtttga
    11881 gcatgccggc ggatcccgcg attatctgct cgaaatctac gagctgcgcg gcatcggggt
    11941 ggcgacgcgc gaaggcgagc agctgacgag tcaaggaggc acagcgctcc gcggcgcgag
    12001 ccatctcgtc gaaactctgg ctgctcgacg cgctttgttc ggaagaccgc aataggtcca
    12061 gattgccctg gatcacgacg agataattgt tgaaatcgtg agcaatcccg cccgccagag
    12121 ttcccaccgc ctccatcttc tgggaatgcc ggagcctttg ctcgagctcc cagcggccgg
    12181 tgacgtcctg caaggtgccg agaacggtgc cgctctcgtc gagcttggcg tccatcgtca
    12241 tatagcgagt ggctccgtct gggcgcacga tgcggaaggt caagggcggg ggcgctacac
    12301 cgcgcgcaat ctgagcccgc gcagcttccc acgcggcgag gtcttccgga tgcacgaact
    12361 tcgggagctc actcgagtct ccggctccag attcaacgcc gataagtggg gtgctcgtct
    12421 ccgaccactc gaagcgccct tgctcacgac gccaaatcca gctgctgatc cccgcaacgc
    12481 ggtgcgcctc gtcgaggagc cgagcctgac ggcgcagttc ctcttcgttc agcacgaacg
    12541 cggttacatc gagcacggca cccacgagcc taacgagctg gccctcggca tccagaatcg
    12601 ccgaaccaat cagcttgatg tgccgcagag agccgtccgg ctgcaaaact cgacaactca
    12661 ccccgggctc cgtcaagccc gtactcgagg tgacctgcga gcgcgccctc acccgctccc
    12721 gatcttccgg atgcagcgcc gcgaagaaca actcaacgct ggccgcttga tcggcggggt
    12781 aacccaatat tcgatacagc tcggcggacc atttgacttc attggtccga aggtcccaaa
    12841 accagctgcc taccttcgcg aggcgctcga cctcttcgta ggcagcaaga cgctcggtca
    12901 gggcctccgc aagccccgac tgggcctctt cctgactcaa gcgccgggtg cgcagcctcg
    12961 ctacctctga ctccagctcg cggatgcgtt gccggagctc gatgagttcg ggagagacga
    13021 gttcggggga ggggcggtcg gaggacaggg cgatgacacg aatgatacac ctccccgtag
    13081 ctccacccgt ccgcgatttt acgggcaagg ggcggagggc tacgagcggc tcattcgcca
    13141 acctcgcgca atctcggcta agacgcgcgc ggcgccgaac tcatcgaggc tgcggcggga
    13201 ctgcagtcga gagcaattgc gcctgcccgc ccccagactc acctcacagg cctaccgcga
    13261 tgctctgcgc gtggtcgaga tgtgcttgca ccttgggcct cagcttctcg agcaatgagt
    13321 tcagctcctc gttgtccgta gtcaaagtga gcgcctgatc aatcagcaat agcgtctcgg
    13381 tgtgcatctc gatttgaacg ctgatatacg cgctgtcgaa cgccgctccc gagagcgcgc
    13441 tgagttcggc ggtcttgctc gcggccttct gctgaaggcc ccggctcaca ctgctctctt
    13501 gaatcgaggt cgactgagcc tcggccaagg cacgggcctc cgcgcgggcc gcggtatggt
    13561 cggtgaccat ctgttgcgca aaggcttgga catccgcgtt ggaggcctgt tcgagcgcta
    13621 aattgccctg ctcgatctcg cctgcattgg cagtgtcgag cacgccgaaa acctgagcgt
    13681 cgctcaaatt gctgggactg gtgctcgacc cgccctcgct ggccccggcg ttaccacctt
    13741 gggagttgcc cgctccgcta cgaccaccag cgctagctcc ggcaacagcg ccgctgccac
    13801 cccctcccca ctgcacgacg gaagaggcgt cctcgtcgtt gctacacgag gcagccaaac
    13861 ccattgccaa gagcacggat gtacgaatcg cagtttttgc aaacatggtt tctccttgcg
    13921 cggcgggcga ctgcacagcg cgtaccaggg ctccgggccg tcatgccccg tagaacgctg
    13981 ccgcggcggc gctacccacc ttgctttgga tccgccctgg aggacgcgag ctatcggccg
    14041 ccggcgacta gctcctattg ggggaggctc ttagcgcgga atcacgcatc cacaccgaag
    14101 gctcgttcga gcaggagggc ttgcggagtg agggccgggt cgaagcagcc tcgataaacg
    14161 acttttcccc gagctctgcg gcgcgcggag ctcgcgcgca gagttcgatc cggagcggcg
    14221 gaggacttcg taaaggactc atcctaagtg cgggcgaacc ccttgatcta cgagttcatg
    14281 acgccgcgag ttcacgtatc gaggcatgcg tacatcggag tggagcgagt ttcagccggg
    14341 cgggttgata aatgggcatc agccgagatc agttccgcca aagccggaca ttaccaacag
    14401 cgagtttgga cctgcggagc cgatgaccgg cggagcgaac gagcctacgg agccgatgaa
    14461 tcctcgggat cgacgaagaa taccgagcga ctgcacacgc agaagcaata cataccactc
    14521 ggca
//
LOCUS       DASTKU010000444         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_530989, whole genome shotgun sequence.
ACCESSION   DASTKU010000444 DASTKU010000000
VERSION     DASTKU010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530989"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(367..924)
                     /locus_tag="VFQ61_15235"
     CDS             complement(367..924)
                     /locus_tag="VFQ61_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955863.1"
                     /translation="MAPSHLLPRGFRRLPGHAPPGCPLLALVLLVASQAHAEQGPTLV
                     YANTSTSECGTLWVGHSGLRRDPRDSHFERVFAPHNPARCEAILQSIGRPNTETPCSQ
                     LQQVLNTADYDPQRLCEAFGYRFAGTLPAVEHACYPALGVLGNPCAPGIWYTLTVLAV
                     LLLGVSFALRRVWILRRRRASLLKE"
     gene            complement(1033..>2271)
                     /locus_tag="VFQ61_15240"
     CDS             complement(1033..>2271)
                     /locus_tag="VFQ61_15240"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955864.1"
                     /translation="DAAGGVTCLACDELDRDAPLHTLGKVVAPRWFSGGSECLVEPAG
                     TVLCSGAIPPAGNGAALRDAPDHRLAGIVGASEVRFNAEDVCTREPAQPWSCASRGRD
                     GLQSAHPNNSMRFAAPELITDAHRTTSVAVHRFALCWIDEGRRATCERRSPAGAKNGT
                     VEIENVRRVVVGNPHACAINTHDELWCFGTNESGQALGVPRPPIALKPTKVLDSVLDV
                     AVGLSHTCAIEQTGQIVCFGDNSRGQLGLKGTLDRQGMWRVPELGETAKQIVAGYEQT
                     CALTEPGHVVCWGAHTGAGDGVRPITGLGAKVLQLAASGRQTCALLEGGTVACWKDNT
                     SVPELIRGSERSIEIAVGDTHACVRTQGGSVRCWGSNEFGQLGRDLPARRIEVRPPHG
                     CIQVDYSIDSESSELGDVAW"
BASE COUNT          438 a          733 c          688 g          412 t
ORIGIN      
        1 tttcttctcc cacgggctcc tccacgggat acaccgtcgg gtcatcagga gttgggggcg
       61 gagtgcgcat gagctcgaac gataccacgc gcgtggcgcc ccgccgaccg gcagtgtgca
      121 tgcagggcta tttagcacga gggtacgaga atgcctgaac aaagagcaca aatttccgca
      181 ggaaccaggg ttatcccgca gctcgcaaca caggcactag ggatatcccg agtgccgtta
      241 atagccggct tgggtatcga gcgtcttcgc cccgatatgc agcgcggagc gcatcccgcc
      301 gaagagaact ccagcaccga gcaaccattt gcgacggagt tttgtcactg ctgagacgac
      361 gccactctac tccttcaata gggacgccct tcgcctcctg aggatccaga ctctacgcaa
      421 cgcgaagctc acgcccagaa ggagcacggc caagacagtg agcgtgtacc aaatccctgg
      481 agcgcacgga ttcccgagca cccccaacgc cggataacaa gcatgctcga ctgctggcaa
      541 cgttccggcg aaacgatacc cgaacgcttc gcacagccgc tgggggtcgt agtcggcggt
      601 gttcaacacc tgctggagtt gcgaacaggg tgtttcggtg ttggggcgcc caatcgactg
      661 cagaatagct tcgcagcgtg ccgggttgtg cggcgcgaac acgcgctcga agtggctgtc
      721 tcgcggatct cggcgcaagc cgctgtggcc gacccagagt gtgccgcatt ccgaggtcga
      781 ggtgttcgcg taaacgagag taggcccttg ttccgcatgg gcttgggatg ccaccaagag
      841 cactagcgcg aggagagggc acccgggcgg cgcatgaccg ggcaagcgtc taaagccgcg
      901 cggcagaagg tgagagggtg ccattgactg agacgtgctg ctcgttctga tgagctggtg
      961 tctgcgtttc cagatgcgcc ccctgttcgt taccacgcac gcggccccct ataacgcctt
     1021 cgcctcgtgg cgctaccaag caacgtcacc cagctcactg gattcgctat caattgaata
     1081 gtccacctgg atgcatccgt gcggcggacg aacctcgatt cgacgtgcgg gcaagtcccg
     1141 accgagttgc ccaaactcgt tcgagcccca gcagcgaact gaaccgccct gcgtccgcac
     1201 acaggcgtga gtatccccca cagcgatctc gatgctccgc tcgctgcctc gaatcagctc
     1261 gggaacggac gtgttgtcct tccagcacgc gacggtgccg ccctcgagta gcgcacacgt
     1321 ttgccggccg ctggcggcaa gctggagcac cttggcccca agccccgtga tgggccgcac
     1381 gccgtcgccc gctcccgtat gagcgcccca acagacgacg tgaccaggtt cggtcagcgc
     1441 gcaagtctgc tcatagccgg ccacgatctg tttcgcggtt tcaccgagct cgggaacacg
     1501 ccacatgcct tgacggtcca aagtgccttt gagtccgagt tgcccacggc tgttgtcgcc
     1561 gaagcaaaca atctgccccg tctgctcgat cgcgcaggtg tggcttagcc cgaccgcgac
     1621 gtcgagcacc gaatccaaga ctttggttgg cttcaaagca atgggcgggc gcggcacccc
     1681 gagtgcttgc cctgattcgt tggttccaaa gcaccagagt tcgtcatggg tattgatggc
     1741 gcatgcgtga gggttgccta cgaccacgcg ccgcacgttc tcgatttcca ctgttccgtt
     1801 ttttgcacca gcaggagagc gtcgctcaca agtcgcgcgt ctgccttcgt ctatccaaca
     1861 gagcgcaaac cggtgaacgg cgacgctcgt ggtgcggtga gcgtccgtga tgagctcggg
     1921 cgcggcaaag cgcatagagt tgttgggatg ggctgattgc agtccgtcgc gacctcgact
     1981 tgcgcagctc cacggttgcg cgggctcgcg cgtgcacacg tcttcagcat tgaaacgcac
     2041 ctcggaagca ccgacaatcc ccgcgagccg atgatcggga gcgtcgcgca acgcggctcc
     2101 gttgccggca ggaggaatcg cgccgctaca caggacggtc cccgctggct cgaccagaca
     2161 ctccgagcca cccgaaaacc atcgcggtgc aacgactttc cccagtgtat ggagtggcgc
     2221 gtcgcgatcc aattcatcgc atgccaggca cgtgacgcct ccggccgcgt c
//
LOCUS       DASTKU010000445        21937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1646160, whole genome shotgun sequence.
ACCESSION   DASTKU010000445 DASTKU010000000
VERSION     DASTKU010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 21937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21937
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646160"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..2059)
                     /locus_tag="VFQ61_15245"
     CDS             complement(176..2059)
                     /locus_tag="VFQ61_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955865.1"
                     /translation="MHHHNSIRYLRLLALLVSPLSLAAKCDSAISIGQHGVAGGVGTG
                     GGSGTGGSNTGGAQTKSCTANDCKSPPPKSATYLCDDGSTAGPMCTLSAAGECAWTVR
                     SCALPPALFSCKDGAEIAVSQKQVCDGVKQCPDGSDEWDCSVSNGWLCADKKTVIAAS
                     AVCDGTKECPDGSDEASCTLPGTWLCADQKTLIKDLQRCDNTKNCPDGSDEVDCSQPG
                     TWLCSDKKTLIKDFQRCDNIKDCPDGSDEAECSQSGTWLCSDGKTLIKDSQRCDNIKD
                     CPDGSDELNCTQPGTWLCSDKKTLIKDVQRCDDIKDCPDGGDEAYCALSATWMCADKK
                     TTIKYAQRCNLIEDCPDGSDEVDCSQPSTWLCSDQKTLIKYSQRCDNIKDCPNGSDEV
                     DCSQPGTWLCADKKTLVKDSQRCDLMKDCPDGSDEDQCVTPPSEFKCDDGTVLFVAES
                     PVCNGVVNCPDGSDESRCPNREFLCKSDPRLGVSLIQICNGKEDCPDGSDEASCSPAA
                     FVCADGSKPTMLEVCDGVKDCADASDEKGCGAGDSPCAGLQSESPFDAARGCLQKPVV
                     IGCYELVSPEIAVPYYPGNHCARRKSDGALFYVATSTVSPRDWVDCSTAEQMAVNNAL
                     PCP"
     gene            complement(2132..3082)
                     /locus_tag="VFQ61_15250"
     CDS             complement(2132..3082)
                     /locus_tag="VFQ61_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9955866.1"
                     /translation="MKDLSRDARALLERTRPTHEPKPEDRARVRRALASHAAGAAAAT
                     SLVGHAKAAAGGAGAAKAASVPAAVLPWLLGGVIAGSASAGYFRLASPSLSETQPGVA
                     ASSSRASANPERRDERGSERSARPVDLEPAPGGVTEGLISPFSAGSTASAPPPLRAPE
                     RGVALVGSKDTEAHRPALPPNNAVVPPHMDLGAPGSQGGTPTTGLTAGESQSGSVAGP
                     GAVASVSAGSDLSEEARALAEAQRALSRGAPRVALSLLAEQSQAFPTGALREERAAAR
                     IFALCASGDAARARSEAARFLRNSPHSPLAARVRAACAKP"
     gene            complement(3079..3660)
                     /locus_tag="VFQ61_15255"
     CDS             complement(3079..3660)
                     /locus_tag="VFQ61_15255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9955867.1"
                     /translation="MSIPRPAAWAEPAIDPAVDVPRLPTFDELYESYFSFAWRTLRRL
                     GVRAEDVADAAQDVFIVVDRRHLDLRSAEQARSWIYGIVVRVAHKYRRSQTRLARGEA
                     QSEELRDERALSAHAAVERAEDVLLLDHLLGKLDLAKREVFVLVELEQMTAVEVAELT
                     QAHLGTVYSRLRAARAEFEEALARHRATQRSLR"
     gene            3916..3987
                     /locus_tag="VFQ61_15260"
     tRNA            3916..3987
                     /locus_tag="VFQ61_15260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3948..3950,aa:Gly,seq:gcc)
     gene            4704..5630
                     /locus_tag="VFQ61_15265"
     CDS             4704..5630
                     /locus_tag="VFQ61_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955868.1"
                     /translation="MKAVLLLIALICGVVALPSPARACSCLVPDKARSYDNADHVVHV
                     IIQRSFINTSSTRSYIARLVDDDYKGCLNRSQRVIIETPASSAACGMSLTVGKEYLLH
                     GQKKGSLLGTPRLSVILCDANTEWSSLTPDHSAFLNTRYVCCGGECSCTDGSQPVNCF
                     VDPCQVSSCDVDGAKCQANYCGGCNAEWRDPSGALVCENKATCDYSSPLKKYVSKDPE
                     QCKLIDFLCVSGSPFFDDCGCGCSVPPAGDCKRSGCSGQLCVEPGDDRVTTCEWREEY
                     ACYQTASCERQPTGACGWTPTPELKACLEQAR"
     gene            complement(5786..6490)
                     /locus_tag="VFQ61_15270"
     CDS             complement(5786..6490)
                     /locus_tag="VFQ61_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HET9955869.1"
                     /translation="MQNLSGFPVTQRWPAKHPDYLQLYSLNTPNGAKVSILLEEIGLP
                     YEAHLVDFNSDDQKTPEFLSLNPNGKIPAILDPNGPGGRPLALFESGAILIYLAEKTG
                     QLLSSDAAARYRTIQWVMFQMGGIGPMFGQVGFFHKFAGKDFEDKRPRDRYVNESRRL
                     LGVLEAQLQDERWLMGDEYSIADIATFPWVRTLTTFYGAAELVGFQDFPHVNRVLTAF
                     LERPAVVRGLQIPKRG"
     gene            complement(6699..8213)
                     /locus_tag="VFQ61_15275"
     CDS             complement(6699..8213)
                     /locus_tag="VFQ61_15275"
                     /inference="COORDINATES: protein motif:HMM:NF016345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HET9955870.1"
                     /translation="MFAGNQSMIGITRKSVTLGILASATACSGVASDAGPESALDSAT
                     DLGHAEAALTTQQRVLGFEAPLGWTASAGSIGTSTTHTEGATSLSLRNASYSELTSEA
                     LSTLSGVSAELAIDVRPPAAAGWGQLQIFASSATLGWSSYQWLGQASLVGLPAGQFTQ
                     VKVSLPATVQQKLAQSYGDLRIRLAVNAPASSSGWLFDNLHFVGSTPPPPDCSTGSQY
                     TLTLVNEQGVDATHLQNLRCTFFQVYPLLVNRFNAAATRNVTFSFVETGNNPAWADPG
                     TGVVFLNKAYLAGNPLDSDVVVHETMHIVQAGYSGQVEGWIIEGSADYARDRYGLTNA
                     ENHWSIPTGWSAGQHFANGYGEAAAFFKWVDARYRQNQAPVVDALDDIMRTGQYADST
                     WVSLTGKTVTALWQEYSNNQAPVPASSGVTLYRDSNFTGWSFVVGTGTYNATDLGSRG
                     LHDVISSLRVPAGYRVIAYLDSESQGASTQFTGDASYVGDDWNDKISTIVVQRL"
     gene            complement(8504..9139)
                     /locus_tag="VFQ61_15280"
     CDS             complement(8504..9139)
                     /locus_tag="VFQ61_15280"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HET9955871.1"
                     /translation="MAPPRRKKPMLVKPARKLPHPKRPYPWLLALALSFAPALSSSSA
                     VAAERAGIQWAESVKLEGKTLALNGTGVRHATILRIKVYVAALYLERKTHSASEVIKS
                     EQTKRLDLVLLRDVSRSDIVDVWRKGMQKNGADLTKLKPRFDKLVSWMPDLNKGDRLS
                     FLYVPGSGTTVSVKGQRKGTLEGSDFAAALFAVWFGPEPADGDLKSELLGQ"
     gene            complement(9302..11221)
                     /locus_tag="VFQ61_15285"
     CDS             complement(9302..11221)
                     /locus_tag="VFQ61_15285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9955872.1"
                     /translation="MDAAQAFHMLVCRMSTGVVVWQYDEAIASLRLVCANAAASRYAG
                     VDLASKIGQPIREIIPDVNEDRHERYVSLARSSNMGQTLIRFEDTRTDKTTFSIRALP
                     MPEACVGIIFQDLADEAGLLAEREKRFRFLLENSLEAISVTDEAGRHLYTSPSIVKIL
                     GYTSEEMLATPGLLLIHPDDQAHAAAVFRDIMARPGEPIPYQVRARHADGSWRELQVV
                     TVNRLSDPSVRGLVHNFRDVSEKHETEKALRRAEEQLRQAQKMEAIGHLAGGIAHDFN
                     NLLSVVLSYSDMLLLDQKAEDARSELLEIKRAGERAAELTRQLLAFSRKQMLKLQVVD
                     LNQTVTSVARLLQRVLGEDLTLQLRLADELWPATLDPAQLEQVLVNLVANARDAMPTG
                     GTVTVQTKNAVLTEFFVASHPSAKPGPHVSLMVKDTGVGIEPSVRLRIFEPFFTTKER
                     TKGTGLGLSVVLGIVQQCGGFIDVESTPGHGAAFTLYFPRCEAGRVAQPHDANAGSEV
                     RGGAETILVVEDDISVRDVVVSVLSRLGYQVLVAPSAQEAGELAARTDRLDLLITDVV
                     MPGVSGPQVASQLTQLRPSLRVLFISGYADHAAVTDALAQDGFGFLQKPVTPKVLADA
                     VRNLLDRKDRSTKAV"
     gene            11698..12891
                     /locus_tag="VFQ61_15290"
     CDS             11698..12891
                     /locus_tag="VFQ61_15290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9955873.1"
                     /translation="MKNRGCVPKSTIVRWSIQWVLFGTLAACGTSRAPEASGGSLGAG
                     GAVAGTTAGASSGASGGGDTLAGGTAGTGGGAASGGSPKGEASGAATSAGANSGSSSG
                     GRIATGGVAGANGGSSQGGANGGASSAGATSAGGGGSAGSSSTGPGGTGGATAGTGGS
                     AGSPHSGVWRIMPLGDSITQSTCYPQLLSQKLRSDGHTNFEFLGSTTNNQSCSGAPNV
                     KTEGHGGYLVTCVTGDRTTGCASKGSPAELDAWLSAKPAPDVVLMHYGTNDAWTSDIP
                     IATITGAYTKIVTKLRAANPNVIIFAAQILPMHPDNCVDSNASCPNVRVKQLNAAIPD
                     WATLASTASSPIYVVDIYSSVGNADNFKPNSADTADGVHPNPSGSAKMVSVWDAALGA
                     RAIPL"
     gene            13033..13755
                     /locus_tag="VFQ61_15295"
     CDS             13033..13755
                     /locus_tag="VFQ61_15295"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9955874.1"
                     /translation="MTDHATVDELTEPRQRRLLVRMVAGAAALALGSCIQSARARGHT
                     DVARANHVPAEVTPGEDLMQEHGLIERVLLVYEEAARRVELGQPLEIGVLSAAIGIVR
                     RFVGDYHERLEERFVFPRLESARREMKLVQVLRSQHERGREVTRALQVRVERGEVNPE
                     LASLLRGFVRMYRPHAAREDTILFPAFREVVGGMAYRELGEQFEAQEHAELGRSGFEG
                     MLSEVEKLEDALGIQDLSRFTI"
     gene            complement(13766..14530)
                     /locus_tag="VFQ61_15300"
     CDS             complement(13766..14530)
                     /locus_tag="VFQ61_15300"
                     /inference="COORDINATES: protein motif:HMM:NF005681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9955875.1"
                     /translation="MGSLSNRVAIVTGASSGIGRATAQLFAAEGACVVLAARRKPELE
                     YLVEEIRQRGGSAVARAGDVTDEAYAEELVRTAIESFGGLDIAFNNAGTLGAMGASTE
                     LSVADWEHTLRTNLSSAFFAAKYQLPAMLGRGRGSLIFTSTFVGHTAGFPGMAAYAAS
                     KAGLIGLTQALAVEYGAKQIRVNALLPGGVDTPMGRVVANTPEALGFVRGLHALKRVA
                     QPEEIAQTALFLASDASSFTTGTALLADGGVSICRT"
     gene            complement(14652..15689)
                     /locus_tag="VFQ61_15305"
     CDS             complement(14652..15689)
                     /locus_tag="VFQ61_15305"
                     /inference="COORDINATES: protein motif:HMM:NF045039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955876.1"
                     /translation="MSGLLIASACSKNSSSNPEGSGGNPAGTGGASLSGGATAAGRNT
                     APGGSGAGASSSGNPSGGATSSGGNSTTAGGTPASGGASVASGGGATNGGATNGGAMT
                     SSGGSTGTSDPCKGALICDDFEAYTDRPAGPWKLSTNNGTVAIDTTRHASGSKSVKFS
                     TTGANSYQRAYIGIEGTPFPLSGNAFYGRMLIYVTQAANDGVHWTMIQADGPVAAQGV
                     TNAQVRYGGQHMQRLMANYDSAGKKSDCWKHSQTKMPEGKWACMQWYFDGASNTQKFW
                     LDGAAIDDLTVTGTGEGCLNHDLNDTWYFPQSFEKAYVGWESYQKDDAREVWIDDVAL
                     GLTQISCPSLP"
     gene            complement(15869..16513)
                     /locus_tag="VFQ61_15310"
     CDS             complement(15869..16513)
                     /locus_tag="VFQ61_15310"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9955877.1"
                     /translation="MPRIKQKTAGNYHHGDLRQALIEAALRTLEKQRAEDLSLRALGR
                     DLGVSPRAPYRHFETKEDLLAAVAVEGFRRFSTKLAQRLQGESESDSVAQLRAIAETY
                     VVFALEHPAAFRVMYAPYATVRERAPELLRARAEAHRSSQEVIARAQAAGQLREDDPM
                     HLALVLWSSMHGLSVLITEGQLGRFDRPVDAAKLAHMVSGLILQGLLKHSRAID"
     gene            16605..17861
                     /locus_tag="VFQ61_15315"
     CDS             16605..17861
                     /locus_tag="VFQ61_15315"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HET9955878.1"
                     /translation="METSGEQKLPFHLTGAFAPVGEERTERELPFTGRIPLELRGTFV
                     RNGPNPRTGWSPAWFAGEGMLHGIRIEAGRAAWYKCRWIKGPRAPNTSIVRHAGRVLT
                     LVETSLPVEVTPELETIAPYDFDGAVMGSMTAHPKTCPRTGELIFFSTSRTPPYLTYF
                     RADAAGRVIHRTVIELAAPAFMHDFAITERYTIFYVPPVRVGDFRSPVPLQWLEQVPT
                     RFAILPRAGGAETVRWFEVAPCSITHIVNAFESDGRVVVDAVSGPRIMASHSWRRYRF
                     DLGSGRATESEVDSRFVDFPRVHPAFVGVRQRYAYTVELRDFGADGSFTKTIARQHDL
                     ERGTATAQDFGAGSMPGEGVVAPRPGFASEQDAWILQLVHRKEGAETALVILNAADYE
                     APPVASIRIPGRVPYGLHAEWLPDLA"
     gene            17947..18603
                     /locus_tag="VFQ61_15320"
     CDS             17947..18603
                     /locus_tag="VFQ61_15320"
                     /inference="COORDINATES: protein motif:HMM:NF014590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955879.1"
                     /translation="MKPRVHNLVSYCGAFLVFALVLGWRWSTRQASWQPSLALSAGAS
                     MWLLHFARRAAESAWVHRYAKPGVPLGDVLVEYVYYWGFALWNALSLSSPNYHGPTSA
                     VVLSAALVFGLAELGNARAHRMLRDLRAPGSAARVVPRGFLFEHVSCPHYLCEILSWV
                     GFALATQTWAALGFLLVGSGILAAWARTRHLAYLKEFDGSEGRELYPSTRRALIPGIF
                     "
     gene            18794..>21937
                     /gene="ileS"
                     /locus_tag="VFQ61_15325"
     CDS             18794..>21937
                     /gene="ileS"
                     /locus_tag="VFQ61_15325"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET9955880.1"
                     /translation="MSVKSAPKYRDVSPELDLPGLEHRILKFWEENRCFQLLREQNAG
                     KPPWSFLDGPITANNPMGVHHAWGRSYKDMFCRFHAMQGRELRYQNGFDCQGLWVEVE
                     VEKELGFKSKRDIERYGIAEFVERCKERARRFAAVQTAQSIRLGYWMDWENSYYTMSD
                     ENNYTIWAFLKRCHEHGWVYKGRDVMPWCARCGTGLSQHEIATEGYRELTHVSPYVRF
                     PLKGREREYLLVWTTTPWTLSSNVAAAVHPNKRYSKVKQGDAIYYLIESREHVLKERG
                     AFERVESLLGSELVGLEYDGPFDELEAQNGVKHRVVAWEEVTDQDGTGIVHIAPGCGK
                     EDFELSKHEGLAVIAPLDEYGVFGPAFGPFANREASTILDDVLANLKAKGLLYKQERY
                     THSYPVCWRCGSELLFRLVDEWYIRMGEQLDKPDTELTAEEKASNLRYQIMDSARQAT
                     WIPSYGLDREIDWLRNMHDWMISKKRYWGLALPIFECSSCGKFDVIGGREELRERAVS
                     GWSEFEGNSPHRPWVDGVVISCKHCGGHATRIKDVGNPWLDAGIVGFSTMQWRDDPEY
                     WKKWYPAELVTESFPGQFRNWFYSLLAMGTVLSGQAPFRRLFGYALMKAQDGREMHKS
                     WGNSIEFNEAADKAGVDAMRWLFASHIPDQNLLFGYDAVDEARRKFLTLWNVYSFFVT
                     YAKIDGFDPQSAPVPLAQRPALDRWILGGLQRLVTVAHSAWEQYQLHTFMRQLEKFID
                     ELSTWYVRRSRRRFWKSETGADKLAAYQTLHEVLVTVTELLAPITPFVSEEMYQNLVR
                     SWDESAPVSVHHRSFPSARSELVDAKLAEDMAAVLQIVELGRRARQNAAIKVRQPLAK
                     MAISLPAEMEQSRLAEFESHILEELNIKAVEYMGNMSPYLRRSLKLDARKAGPRLGAA
                     TQSVLAAVRKLPPEQLASHTPGAPLEVTANGVTYTLTAEELVLDEAAVAGFAYAGNGR
                     VSVLLTTEITEDLRREGAVRDVIRLVQEMRKNHKLEVADRISLELEGSEAMRNAVQAQ
                     LELLKRETLAVEVNFRAT"
BASE COUNT         3909 a         6729 c         7043 g         4256 t
ORIGIN      
        1 atcttgggag ggcacccgtt cgtgatcgat cgagcagggg cgaaagaaca ggtccatcgc
       61 ccaggtcgat tctcggggcg cggcgggttg gccctggaat tcgtgtttta ggcctggcag
      121 gccgcgaccg ccgttcggtt cgagcgagtc tggcggcctg ccggggcgac tagacttagg
      181 ggcaggggag agcgttgttg acggccattt gctccgctgt cgagcaatct acccagtccc
      241 gcggggacac ggtggaggtc gcgacgtaga acaacgcccc gtcggatttg cgacgcgcgc
      301 agtgattgcc cgggtaatag gggaccgcga tctccggcga gacgagctcg taacacccga
      361 tcacgacggg cttctgcaag caacctcgcg cggcgtcgaa cggactctca ctctgcaggc
      421 cggcgcaagg cgagtcaccc gcgccgcaac ccttctcgtc gctcgcatca gcacaatcct
      481 tcacgccgtc gcacacctcc agcatcgtcg gcttggagcc gtcggcgcac acgaaggcgg
      541 caggcgaaca gctggcttcg tcgcttccgt ccgggcaatc ttccttacca ttgcagattt
      601 ggatcagcga aacaccgagc ctcggatcac tcttgcacaa gaactcgcgg ttcgggcagc
      661 gagactcgtc cgatccgtca gggcaattca ccaccccgtt gcagactggc gactcggcaa
      721 cgaagaggac ggtgccatcg tcgcatttga actcgctcgg gggcgtgacg cactggtctt
      781 cgtcagagcc gtcggggcag tctttcatga ggtcgcaacg ctgtgaatcc ttgaccagcg
      841 tcttcttgtc ggcgcaaagc caggttccgg gttgagagca gtcgacctca tcgctgccgt
      901 tcgggcagtc tttgatgttg tcgcagcgtt gcgagtactt gatcaacgtc ttctggtctg
      961 agcagagcca agtgctcggc tgagagcaat ccacctcatc gctgccgtcg gggcagtcct
     1021 cgatgagatt gcagcgttgg gcgtacttga tggttgtttt cttgtcggcg cacatccatg
     1081 tggcggacag agcgcaatac gcctcgtccc cgccgtcggg gcagtctttg atgtcgtcgc
     1141 agcgttgcac gtctttgatg agcgtctttt tgtccgaaca gagccaagtc cccggctgtg
     1201 tgcaattgag ctcatcgctg ccgtcagggc agtctttgat gttgtcgcag cgttgcgaat
     1261 ccttgatcag ggtctttccg tcggaacaga gccaggtgcc cgactgagag cactcggcct
     1321 catcactgcc atcagggcag tctttgatgt tgtcacagcg ttggaagtct ttgatgaggg
     1381 tcttcttgtc cgagcacagc caagttcctg gctgagagca atcgacctca tcgctgccgt
     1441 cggggcagtt cttggtgttg tcacagcgtt gcaggtcttt gatgagggtc ttttggtcgg
     1501 cgcagagcca ggttcccggc agtgtgcagc tggcctcatc gctgccgtca ggacattcct
     1561 tggtcccgtc gcagacagcg ctggcggcaa tcacggtctt tttatccgcg caaagccagc
     1621 cattggagac ggagcagtcc cactcatcgc tgccgtcggg gcactgcttc accccgtcgc
     1681 atacctgctt ctgcgacacg gcgatctccg cgccgtcttt gcaggagaag agcgcaggcg
     1741 gcagcgcgca cgagcggacg gtccaagcgc attcgcctgc ggcactgaga gtgcacatgg
     1801 gccccgcggt ggagccgtca tcgcacagat aggttgcgct cttgggcggc gggctcttac
     1861 agtcattcgc ggtacagctc ttggtttgag cgccacccgt attggagccg ccggtgccag
     1921 aaccaccgcc ggtgccaact ccgccagcta cgccgtgctg cccgatcgaa atggcactgt
     1981 cgcatttggc ggctaacgaa aggggagaaa cgagtagcgc cagcaggcgc aggtagcgaa
     2041 tggaattgtg atggtgcatg tgcggcctcc tcacgcgggc gatcgcgttc ggtgagctat
     2101 gcgtggacac gcggggaacc atttgagttt gttacggctt cgcgcacgca gcgcgcactc
     2161 ttgccgcgag cggcgagtga ggcgagttcc tgagaaagcg cgctgcctcg gagcgggccc
     2221 gagctgcatc acccgatgcg catagggcaa atatgcgtgc tgcggcccgc tcttcacgca
     2281 acgcaccagt tgggaaagcc tggctctgtt cggcaagcaa gctcaacgcg acgcgaggtg
     2341 ctcctcggct caaagcgcgc tgtgcttctg ccaacgcccg cgcttcctca ctcaagtcag
     2401 agcctgcact caccgaggcc acggcgcccg gtccggccac agacccgctc tgtgattcgc
     2461 ccgccgtcaa tcccgtcgtc ggtgttcctc cctgcgatcc cggtgctccc aggtccatat
     2521 gaggcggcac cacggcgttg ttcggcggca atgccggacg gtgggcttcc gtatccttgg
     2581 accccactag cgccaccccg cgttctggtg ctcgaagcgg gggcggcgca ctcgcagtgc
     2641 ttccggcgga gaagggggag atgaggccct cggtcactcc gcccggagct ggttccaaat
     2701 cgacgggccg cgcgctgcgc tccgaaccac gttcgtcgcg acgctccggg ttggcactcg
     2761 cgcggctcga gcttgcggcc actccgggct gggtttccga caggctcggt gatgccaaac
     2821 ggaaatagcc ggcgctggcc gaacccgcta tcactccgcc cagtagccac ggaagcacgg
     2881 ctgccggaac gctagcagcc ttcgccgctc cggctcctcc ggcggcggcc ttggcgtgcc
     2941 cgaccaaaga cgttgcggcg gccgcgcccg cggcgtgcga ggccaatgcg cgccgcactc
     3001 gagcgcgatc ttcgggcttt ggctcgtgtg tcgggcgagt tcgctccagg agcgcgcgag
     3061 cgtctcggct gaggtccttc atcgtagact ccgttgggtg gcgcggtgtc gggctagggc
     3121 ctcctcgaac tccgcgcgag ccgcgcgcag ccgcgagtac acggttccga ggtgcgcctg
     3181 cgtcagctct gccacctcca ccgctgtcat ctgctcgagc tcgacgagca cgaagacctc
     3241 tcgtttggcc aggtccagct tccccagcaa gtgatccagc agcagcacgt cttccgctcg
     3301 ttccacggcg gcgtgggcgc tcagggcgcg ctcgtctcgg agctcctcgg actgcgcctc
     3361 tcctcgcgcg agtcgcgtct ggcttcgacg atacttgtgg gccacccgca ccacgattcc
     3421 gtaaatccac gagcgggcct gctcggccga gcggaggtcc aaatgtcgac ggtccacgac
     3481 gatgaacaca tcctgcgccg cgtcagccac atcttcggcg cgcaccccga gccggcgcag
     3541 cgttcgccag gcaaacgaga agtagctttc gtagagctcg tcgaaggtcg gcaagcgcgg
     3601 aacatccaca gctgggtcga tagcgggctc agcccacgcg gccggtcgcg ggatgctcat
     3661 gggtcatgca gcaatgaccg ctcgagggaa cttccatttc gaattcctac gccggccttt
     3721 tacgtgaccg ccccgcgact cggtgcgcaa tccggggctg gctttcgaga ctagctcggc
     3781 cagctcggcg ctatttttca gggctcacgt gtcgtcccag gggccagtgc tcagcgtcct
     3841 acttttttct ggggactcgc ttgacagacc ccccggataa agttaaacag cgcctccctg
     3901 ttacgggaac tcatcgcggg agtaactcag cggtagagtg ccaccttgcc aaggtggacg
     3961 tcgtgggttc aaatcccatc tcccgctccg aaatttcgga gtcatccgca gccccaacga
     4021 gagaatctcg agtgaggcac cgagccggtt aggattcagt cctagccggc tttttcgttt
     4081 tgttctcggc cgagtgcgcg gcgcgccctc ccttccggtc ggtccgcggc gattctcgca
     4141 cgcgctcggg aaccagtccg cggcgcctac gggagtccct ccaaagcaga ctcaagcgcc
     4201 tcgaagcgcg actcgtccgg aaccccgaag cttcgcgcgc ccaaggggca cgggtcgagg
     4261 ccccgacgga gtgagccaga gcacgccgct cgatcggggg acgagagcga cgccgctgca
     4321 gaactagcct tctcgacgtt tattcgttca gtcgtcactc gtcgttggcg cacgggagag
     4381 atggagtgaa tccagtgctg tcgagtctgc ggtgggggcc tgcgcggctg cgttgagcaa
     4441 tgcgggtgtc tgtgattgcc caacgcgccg gtgcggcggg cttcgcgccc gtacgatcgc
     4501 ggccaacgat agacgagcat ccagcaatcg ccgaagagcc cgcgctgggt acaccgggat
     4561 actacgcaat tgccgcaaat gtgccgtctc ggttggcctt tgggcacagt cagagtttgt
     4621 cagtggcagc gcctgtcagg gccgctgatt gtcacttgca accgacgcgg gaaagaagaa
     4681 gatcccgtgc agaggaggtc gccatgaaag cggtactatt gttgatcgcc ctgatctgcg
     4741 gggtcgtcgc actgccttcg cctgcgagag cgtgctcttg cttggtgccg gacaaggcgc
     4801 gcagctacga caacgcggac catgtcgtgc acgtgattat tcaacgttcg ttcatcaata
     4861 cgtccagtac tcgatcgtac attgctcgtc tcgtcgacga cgactacaaa ggttgcctga
     4921 atcgctccca gcgtgtgatc atcgagacgc ccgcgagcag cgcggcttgt ggcatgagct
     4981 tgacggtcgg caaggagtat ctcttgcacg gccaaaagaa ggggtcattg ctggggaccc
     5041 cgcggctatc ggtgatactg tgcgacgcca acaccgaatg gtcttctctg actcccgatc
     5101 actcggcgtt cctgaataca cgttatgtct gctgcggggg tgaatgctct tgcacggacg
     5161 gaagtcagcc cgtcaactgc ttcgtagacc catgtcaggt gagcagttgt gacgtagatg
     5221 gagccaagtg ccaggcgaac tactgtggtg gctgtaatgc ggaatggcgg gacccgagtg
     5281 gcgcgctggt ctgcgagaac aaagccacgt gcgactattc gtcgcctctc aaaaagtacg
     5341 tctcgaagga tcccgagcag tgtaagctga tcgacttctt gtgcgtgagc ggcagcccgt
     5401 tcttcgacga ttgcggctgt gggtgcagcg tgcctccggc cggcgactgc aagcggagcg
     5461 ggtgctccgg tcaactgtgc gtggaaccgg gagacgatcg cgtgaccacc tgcgagtggc
     5521 gcgaggagta tgcgtgttat cagaccgcca gttgtgagcg ccagccgacc ggcgcttgtg
     5581 gctggacgcc cactcccgag ctgaaggcct gcctagagca ggcgcgctga aacagcgagt
     5641 cggattcgcg ttgaggaatg tatcgcgcgc ggggctctcc gagccgctcg ggagccccgg
     5701 tagtcgggat ctttggagct ccgcgccgat acgctagtga gtagaagacg tgattacttg
     5761 ggcgtgtgcg cgagcagatt gcgtgtcagc cgcgcttggg aatttggagc ccgcgaacta
     5821 ccgcggggcg ctccaggaat gcggtgagta ctcggttcac gtggggaaag tcttggaagc
     5881 ccacgagttc agccgctccg tagaacgtcg tgagcgttcg aacccacgga aaggtagcga
     5941 tatcggcgat gctgtattcg tcacccatga gccagcgctc gtcctggagc tgggcttcga
     6001 gcactcccaa taagcgccgg gactcgttga cgtaacggtc tcgcgggcgc ttgtcttcga
     6061 agtctttgcc cgcgaacttg tggaaaaagc cgacctgccc aaacattggg ccaatcccac
     6121 ccatttgaaa catcacccat tggatcgttc gataacgggc ggctgcatcc gaagacaata
     6181 gctggcccgt cttctcggcc aggtaaatca gaatcgcacc cgattcgaag agcgcgagcg
     6241 ggcggcctcc tggtccgttc ggatcgagga tggccgggat cttgccattc gggttcagag
     6301 acaagaactc cggagtcttt tgatcatcgg agttgaaatc caccaaatgg gcttcgtagg
     6361 gaagtccgat ctcctccagc aggatcgaga ccttcgctcc gttcggggtg ttgagcgagt
     6421 agagctgcag ataatcggga tgtttcgcgg gccacctctg ggtgaccgga aagccgctga
     6481 gattttgcat gggggctcga tgggtgcgtt cgaatgaata gctcaatgag cggctcaaat
     6541 gaatagtgcc gcccgatcgg ccgtcaaggc cgccaatcta ttgcaatggg cgttgaaccg
     6601 cctgcccaat tgggcgggtg tatcgagccg cggagtggag gcgggccgcg tcaggtgacg
     6661 gctcgcgaaa ccgacacctg acggcggcga gtctcagctc agagccgctg gactacgatg
     6721 gtcgagattt tatcgttcca atcgtccccc acgtagctcg cgtctccggt gaattgtgtg
     6781 ctggcgcctt ggctctcact atcgagatag gcaatcacgc gatagccagc cggaacccgc
     6841 agagaagaga tcacgtcgtg caaaccgcga gagcccaagt cagtggcgtt gtaagtgccg
     6901 gttccaacca cgaatgacca gcccgtgaag ttggagtctc gatacaacgt cacgccggag
     6961 cttgcgggta cgggtgcctg attgttgctg tattcttgcc acagcgcggt gacggtcttt
     7021 cccgtcaaac tgacccaggt gctgtccgcg tattgcccag tccgcatgat gtcgtccaag
     7081 gcgtctacga ctggcgcttg attctgtcgg tagcgagcgt ccacccactt gaagaaggcg
     7141 gccgcctcgc cgtagccgtt cgcgaagtgt tgccccgcgc tccagccggt cgggatcgac
     7201 caatggttct cggcgttggt caaaccgtaa cgatcgcgcg cgtagtcagc agagccctcg
     7261 atgatccagc cctccacctg gccggaatag ccggcttgca cgatgtgcat cgtctcgtgc
     7321 acgaccacgt ccgagtctag cggatttcca gccaaatagg ccttgttcaa gaagaccacg
     7381 ccggtgcccg gatcggccca ggccgggttg ttgcccgtct ccacgaacga gaaggtaacg
     7441 ttgcgggtcg cggccgcgtt gaatcgattg accagcagcg ggtacacctg gaagaacgtg
     7501 cagcgcaggt tttggaggtg cgttgcatcc acgccctgtt cattgacgag ggtcagcgtg
     7561 tattggcttc ccgtgctgca atcaggcggt ggcggggtgg agcctacgaa gtgcaggtta
     7621 tcgaataacc acccgctgct cgacgccgga gcattgacgg ccaaccgaat ccgcaagtca
     7681 ccataactct gcgcaagctt ttgctggacc gtcgccggca ggctgacttt cacctgcgtg
     7741 aattgccccg ctggtagccc gaccaacgat gcttgaccca gccactggta gctcgaccac
     7801 ccaagcgtcg cgctggacgc gaagatctgg agttgacccc aaccggcggc ggccggtggc
     7861 cgtacgtcga tcgcgagttc cgcgctcacc ccgctgagtg tcgacagagc ctcggaggtg
     7921 agctccgagt agctcgcgtt gcgcaagctc aatgaggtgg cgccctcggt gtgcgtagta
     7981 ctcgtcccga tcgagcccgc tgaggcggtc cagcctaggg gagcctcgaa tccaaggacc
     8041 cgctgctggg tggtgagcgc cgcctcggcg tgacccagat ccgttgcaga atcgagcgcg
     8101 gactctggtc cggcgtcgct cgcaacgccg ctgcacgcag tggccgaagc gaggattcca
     8161 agcgtcacac tcttgcgggt gattccgatc atcgattgat ttccagcgaa catacgactt
     8221 cctcctcggg agtgagaaca caccgcgagc attgcgcgcc attgccgctc tcactcggtc
     8281 agcccattgg aggtctgcgc tggccaaacc ttcgccgcgc gatcgaaaaa atccggtgct
     8341 acgccgtgct gacgttacgc tcgcgcgcaa tcatgcccac tcgagcacga cgcgtcgttg
     8401 tgtcagagtt gtgcttcagg ggaacgcgac ggctcggatc gagggacgcg gaacgagcgg
     8461 ccgtgtccct gctgatcggc aggcccgggc gggttcgttg gcctcactgc cctagcagct
     8521 cgctcttcag atccccgtct gcgggttcgg gcccgaacca caccgcgaac agggcggccg
     8581 cgaaatcgga gccttcgagg gtacctttgc gttggccctt gacgctgacc gtagttccgc
     8641 tccccggaac atagagaaag ctcagccggt cccccttgtt caagtcgggc atccacgaga
     8701 ccagcttgtc gaagcggggc ttgagcttcg tcaagtccgc tccattcttc tgcatgccct
     8761 tgcgccaaac atcgacgatg tcactccggc tcacatcgcg gagtaacacc aaatccagtc
     8821 gcttggtctg ctccgatttg atcacctcgc tcgcggagtg agtcttccgt tcgagataga
     8881 gcgcagccac gtagaccttg atgcgcagaa tggtcgcgtg gcgaacacca gtcccgttca
     8941 acgccagggt cttgccctcc agtttgacgc tttctgccca ctggatgccc gcgcgttcgg
     9001 ccgccactgc gctcgagctc gagagggcgg gcgcaaacga gagggcgagc gcgagcaacc
     9061 agggataggg tcgcttcgga tgcgggagct tgcgtgctgg ttttacgagc atgggcttct
     9121 ttcggcgcgg tggggccatg aaagaatagc catgctcggg gtgtgcgcaa agctcgagtc
     9181 gcttcgagtc cacgccttta ttgcccgcgc ggcgtgttgc tcgtaatcct cgtcgcgctg
     9241 tcggtcttct ggacacaccg acgcctgcgc gcgagtggca agcgtccgta gatatcggga
     9301 atcaaaccgc cttggtgctt cgatctttgc gatcgagcag gttccgcacc gcatcggcca
     9361 ggactttggg ggtgaccggc ttctgcaaga aaccgaagcc gtcctgcgct agcgcgtccg
     9421 ttacggccgc gtgatcggcg taaccggaga tgaacaatac gcgcagggag gggcggagtt
     9481 gcgttagctg gctggcaact tgcggcccgc tgacgccggg catgaccaca tccgtaatca
     9541 agaggtcgag tctgtcggta cgggcagcca gttcgcccgc ctcttgcgcc gagggcgcca
     9601 ccaatacttg gtatcccaag cgcgaaagca ccgaaaccac cacgtcgcga acgctgatgt
     9661 cgtcttcgac caccagaatc gtttccgctc ccccgcgcac ttccgagccc gcgttggcat
     9721 cgtggggttg cgcgacgcgc ccggcctcgc acctcggaaa atacaaggtg aacgcggcgc
     9781 catggccggg ggtactctcg acgtcgatga aaccaccgca ctgttggacg ataccgagta
     9841 cgacggagag gccgagtccc gtgcccttgg tgcgctcttt ggtggtgaag aagggttcga
     9901 agatgcgtag ccgtacggac ggctcgattc cgactccggt atctttgacc atcaaagaca
     9961 cgtgcggtcc gggctttgca ctggggtgac tcgccacgaa gaactccgtg agaacggcgt
    10021 tcttcgtttg aactgtgacg gtgccccccg tcggcatcgc gtcccgcgcg ttcgccacca
    10081 ggttgaccaa gacttgttcg agctgtgcgg gatcgagagt tgcgggccag agttcgtccg
    10141 ccaaccgaag ctgtaaagtg agatcttcgc ccagcacccg ttgcaacagc cgagccacgc
    10201 tcgtcacggt ttggttcagg tccaccacct gcagcttgag catctgcttg cggctaaagg
    10261 caagcagttg ccgcgtgagt tcggcggcgc gctcgcccgc gcgcttgatt tcgagcagct
    10321 cgctccgagc atcctcggcc ttttgatcca gcagcaacat gtcgctgtag ctgagcacca
    10381 ccgagagcag gttgttgaaa tcgtgcgcga taccgcccgc caagtgccca atcgcttcca
    10441 tcttttgggc ttgtcggagc tgctcttcgg cgcggcgcag cgccttttcg gtctcgtgct
    10501 tctcgctaac atcgcgaaag ttgtgaacga gtccgcgcac gctcggatcc gatagccggt
    10561 tcacggtcac gacctgcagc tcgcgccacg agccgtccgc gtggcgggca cggacttgat
    10621 agggaatcgg ctcaccgggg cgggccatga tgtctcggaa cacggcggcc gcgtgcgcct
    10681 gatcgtcggg gtgaatcagc agcagaccgg gcgtcgccaa catctcctcc gaggtgtacc
    10741 cgaggatctt cacgattgac gggctggtgt agaggtggcg cccggcttca tctgtcaccg
    10801 agatggcttc caagctgttc tcgagcagaa atcgaaagcg cttttcgcgc tcggcgagca
    10861 acccggcttc gtccgcgagg tcctgaaaga tgatgcccac acatgcttcc ggcatgggca
    10921 gggctcgaat cgagaaggtc gttttgtcgg tgcgagtatc ctcgaagcga atcagggttt
    10981 gccccatgtt gctagaacgc gcgaggctca catagcgctc gtgccgatcc tcattgacgt
    11041 ccggaattat ctcgcgaatg ggttgtccga ttttcgacgc gagatcgacc ccggcgtagc
    11101 ggctcgccgc cgcgtttgcg caaaccagac gcaggctcgc gatcgcttcg tcgtactgcc
    11161 agaccacgac gccggtcgac atgcggcaga ccagcatgtg aaaggcttgc gcagcgtcca
    11221 tcgccgccaa gatactgaca cgttctcacg agtggcaacg ctgcatgagc agcggtatgg
    11281 ccgctggaac ggctgaaatg caggaaatgg agcttgcgcg gcctagcctg cgaaacgtgg
    11341 catctttaca ctttcgaagg gctgggcgag ctggcatcgg agtggagcgt ccttaggtag
    11401 tgacccgagc gcatcgacga tgcgggttcg agtgctgagg ttcgacggga actcgatgcg
    11461 aacggacgtg taggcgcgac cacgatcgcg agcgcagacg cggattctgg gtggaggttc
    11521 tgctaccacg gctgaaatgc aactgagacg cggggtgagg cttcccgcac cgcttggatt
    11581 cgtcaggttc cgagcacgtt cgcgccaggc agggtttccc cgcctcgaga gatgttgcag
    11641 gctgcgctcg tgactcgatc gctcgtcagc tttgcaccgc gcttcagggc tcatctcatg
    11701 aagaatcgcg gttgcgttcc gaagtctacg atcgtgcggt ggtcgattca gtgggttttg
    11761 ttcggcactt tggcggcgtg cggcacgtct cgggcgccgg aagccagcgg cggctcactg
    11821 ggtgcgggcg gagcagtggc aggaacgact gcaggagcga gcagtggcgc ttcgggggga
    11881 ggagacacat tggctggtgg caccgcgggg acaggcggag gtgcagcgag cggtggtagt
    11941 cccaaaggcg aagcgagtgg cgccgctacc agcgccggtg ccaacagcgg gagttctagt
    12001 ggcgggcgga tcgcaacggg cggtgttgct ggtgcgaatg gcggttccag ccaggggggc
    12061 gcgaatggcg gagcgagcag tgcgggagcg accagcgcgg gaggaggagg cagcgccggc
    12121 tcgagcagta ccggtccggg cggtaccggc ggcgctacgg cgggtactgg gggttcagcc
    12181 ggcagccccc acagtggcgt gtggcggatc atgccgctag gagattcgat cacgcagagc
    12241 acctgctacc cgcagttgtt gtcacagaag ctcaggagtg acgggcacac gaacttcgaa
    12301 tttctcggga gcaccacgaa caaccagagt tgtagcggcg cgccgaacgt caaaaccgag
    12361 ggtcacggag ggtacctcgt gacttgtgtg accggtgatc gcacgaccgg gtgcgcgagc
    12421 aaaggctcac ccgccgagct cgatgcctgg ttgtcggcca agccggcacc ggacgtcgta
    12481 ctaatgcact acggcacgaa cgacgcatgg acgagtgata tcccaattgc caccatcacg
    12541 ggcgcctaca cgaagatcgt gaccaagctg cgtgccgcta acccgaacgt aatcatcttc
    12601 gcggcccaga ttctgccgat gcatccggat aactgcgtag acagcaacgc gagctgtccg
    12661 aacgttcgcg tcaagcagtt gaacgcagcg attcccgatt gggccacgct cgcttcgacg
    12721 gcttcatctc caatctacgt ggtcgatatc tatagctcgg tcggcaacgc ggacaatttc
    12781 aagccgaaca gcgccgacac ggcggacggt gtgcatccca acccctcggg cagcgccaag
    12841 atggtcagcg tttgggacgc ggcgctggga gctagggcta ttcccctgtg agcgcggcgc
    12901 ccgtgcgatg agtttggtcg agggcgcgcg cgggaaacac cggtgcgcca aatgtccgtt
    12961 gagtttaggg ggcctttcac gccatctgcg ttgttgctgg tcggcgcccg ggccactgcc
    13021 aagttttgcg gcatgactga ccacgcaacg gtggacgaac tgaccgaacc ccggcaacgc
    13081 cggcttttgg tgcgcatggt ggcaggagcg gctgctctcg cgctcggtag ctgcatccaa
    13141 tcggcccgag cccgggggca taccgatgtc gcgcgcgcca atcacgtgcc ggcggaggtg
    13201 acgccgggcg aggacctgat gcaggagcat ggcttgattg aacgggtatt gctcgtctac
    13261 gaggaagcgg cgcgccgtgt ggaactcggc cagccactcg aaatcggcgt gctcagcgcc
    13321 gcgattggga tcgtacgccg gttcgtaggg gactaccacg aacgtttgga ggagcgcttc
    13381 gtcttcccac ggctcgagtc tgctcgtcgc gagatgaagc tcgtccaggt gcttcgctcc
    13441 cagcacgagc ggggccgcga ggtcactcgc gcgctgcagg ttcgagtcga acggggtgag
    13501 gtgaaccccg agctcgcgag tctgctgcgc ggattcgtgc gcatgtaccg tccgcatgcc
    13561 gcgcgcgagg acaccattct gttccctgct ttccgagagg tggtcggcgg gatggcgtat
    13621 cgagagctcg gcgaacagtt cgaagcgcaa gagcacgcgg agctcggacg ctcggggttc
    13681 gaggggatgc tgtcggaggt cgagaaactg gaagacgcgc tcgggattca ggacctctct
    13741 cgattcacga tctgaagaaa gcgattcaag ttctacaaat cgatacaccc ccatcggcca
    13801 gcagcgccgt gccggtggtg aaactcgagg catccgaggc gaggaaaagc gccgtttgag
    13861 caatttcctc gggctgagcg acgcgtttga gggcgtgcag ccctcgcacg aagcccaggg
    13921 cctcgggcgt attcgccacg accctgccca tcggagtatc cactccgcct ggcaagaggg
    13981 cattcacgcg gatctgcttg gccccatatt ccacggccag agcttgggtc agcccaatga
    14041 gcccggcctt gctcgcggca tacgccgcca ttcccggaaa ccccgccgtg tggccgacga
    14101 aggtagacgt gaatatgagc gagcctcggc cacgcccgag catcgcgggg agctgatact
    14161 tggccgcgaa gaacgcgctg ctcaggttgg tgcgcaacgt gtgctcccag tcggcgacgg
    14221 ataattcagt cgaagctccc atcgccccta gcgtccccgc gttgttgaac gcaatatcta
    14281 aacctccgaa gctttcgatg gcggttcgca ccaactcctc cgcgtaggct tcatcggtga
    14341 catctcccgc gcgcgcaacc gcagaaccgc cgcgctgcct aatttcttct accaagtatt
    14401 ccagctccgg cttgcggcga gctgccagta ccacgcaggc gccttctgca gcgaagagct
    14461 gcgcggtcgc tcgaccaata ccagaacttg ctcccgtgac gatcgccacg cggttcgaaa
    14521 gtgagcccat gattgccttt ctggcgctcc tgaatcgagc gctcgagccg gcattctgga
    14581 gttcggcgtg ccggggctca ctccgcttct tgctctctaa tcgagaattg atctctcgga
    14641 aattgcgcgc ctcagggcag ggatgggcaa gaaatctggg tcaggccgag cgcgacgtcg
    14701 tcgatccaaa cctcgcgcgc gtcgtccttc tgataactct cccagcccac gtaggctttc
    14761 tcgaagctct gcgggaagta ccaagtgtcg ttcaagtcat ggttgaggca gccttcaccc
    14821 gtgccggtga cggtgagatc gtcgatggca gcgccgtcga gccaaaactt ctgagtgttc
    14881 gaagcgccat cgaagtacca ttgcatgcag gcccatttac cttccggcat cttggtctgc
    14941 gagtgcttcc aacagtcgct cttcttgccg gcgctatcgt aattggccat caagcgctgc
    15001 atgtgttggc cgccgtaccg cacttgggcg ttggttactc cttgtgccgc taccgggcca
    15061 tccgcctgaa tcatcgtcca gtgcacgccg tcgttggcgg cctgggttac gtagatcagc
    15121 atgcggccgt aaaacgcatt gccgctgagc ggaaaggggg tgccttcgat tccgatatag
    15181 gcccgttgat aactgttggc gccggtggtg gagaacttca ccgatttgct gccgctcgcg
    15241 tgccgcgtcg tatcgatggc caccgtgccg ttgttggtac tgagtttcca agggccggca
    15301 gggcggtctg tataggcctc gaaatcatcg caaatcaaag cccctttgca cggatccgaa
    15361 gtgcccgtgc tgcctccgct gcttgtcatg gcgccgccat tggtcgcgcc gccattggtc
    15421 gcgccgccac cgctagcaac gctcgcacct ccgctcgcgg gcgtgccgcc ggcagtcgtt
    15481 gaatttccac cgctgctcgt cgcgccaccc gaggggttgc cactcgacga agcgcccgcc
    15541 ccgcttccac cgggtgccgt gttgcgaccc gcggcggtgg cgccgccaga cagcgacgct
    15601 ccgccggtac ccgccgggtt tccgccgctt ccttccggat tcgaactgga gttcttggaa
    15661 cacgcgctcg cgatcaatag gccgctcaag gcgagtccga gtcgccatgc cgtgcggata
    15721 ccgacgccag tctgtggccc ctcgtgcccc gcgcacctgg cccaccgcca cgcaatttga
    15781 accacgcact tcgatcgcat tccctcgaag gctaatcgaa tcgaagaacg aaggataggg
    15841 acgtcgcacc accgagcagg ggtgggtgct agtcgattgc gcgcgagtgc ttcagtaggc
    15901 cctgcagaat cagtccgctc accatgtgcg ccaatttcgc agcgtcgacc ggtcgatcga
    15961 agcggccgag ttgtccctcg gtgatcaaga ccgaaagccc atgcatgctg gaccagagca
    16021 cgagcgcaag gtgcatgggg tcatcttccc gcagctgccc tgccgcttgc gcccgcgcga
    16081 tgacttcctg agagctgcga tgagcttcgg ctcgtgcgcg cagcaattca ggagcacgtt
    16141 ctcgaacggt ggcgtacggc gcgtacatca cacgaaaagc ggcagggtgc tccagggcaa
    16201 acaccacata cgtctctgcg atcgcccgca gttgtgcgac cgaatcgctt tcactctcgc
    16261 cttgcaggcg ttgcgccagt ttggtggaga agcgccgaaa gccttccacc gcgacggcag
    16321 ccaataggtc ctctttggtt tcgaagtgtc ggtaaggggc gcgcgggctc acgccgaggt
    16381 cacgtcccaa cgctcgcagc gagaggtcct ccgccctttg cttctcgagc gtgcgcaaag
    16441 ctgcctcgat cagcgcctgg cggaggtcgc cgtgatggta gttgcccgcg gtcttctgct
    16501 taatcctcgg catttcgagc gtgcccttct tctatcctcc acgcggctct gggttgggca
    16561 gctttttcgc tcgacacggc cgattcgaag tatacagtga atacatggag acctcgggcg
    16621 agcagaaatt gccgttccat ctaacgggtg ctttcgctcc agtgggtgag gagcgaaccg
    16681 agcgggagct gccgtttact ggacgaattc cgctcgaact tcggggtacc ttcgtgcgca
    16741 atggcccgaa tccacgcacg gggtggtctc cggcgtggtt tgcgggtgag ggcatgctgc
    16801 acgggatccg catcgaagca gggcgcgccg cttggtacaa gtgccgctgg atcaagggac
    16861 cacgcgcgcc caacaccagc atcgttcgac atgccggccg ggtgttgacg ttggttgaga
    16921 ccagcttgcc agtcgaggtc acccccgaac tcgaaaccat cgctccttac gatttcgacg
    16981 gggccgtgat ggggtcgatg acggcgcacc ccaagacttg cccgcggacg ggggagctga
    17041 tcttcttttc gacgtcgcgg actccgcctt acttgaccta tttccgggct gacgccgcgg
    17101 gccgtgtgat tcatcgcaca gtcatcgagc tggctgcgcc cgccttcatg cacgatttcg
    17161 cgatcaccga gcgatatacg atcttctacg taccaccggt ccgggtggga gactttcgct
    17221 ccccagtgcc gctccaatgg ctggagcagg tgcctactcg tttcgcgatc ttgccgcgag
    17281 ccggcggcgc agaaacggtt cgctggttcg aagtcgcgcc gtgttcgatc actcatatcg
    17341 tcaacgcgtt cgaatcggat ggacgggttg tcgtggatgc agtgagtggg ccccgtatca
    17401 tggcttcgca tagttggcgc cgataccgct tcgatctcgg gtcggggcgg gccacggaaa
    17461 gtgaggtgga ttctcgcttc gtagatttcc cgcgcgtgca cccggccttc gtgggagttc
    17521 gtcaacgcta cgcttacacg gtggagctcc gggacttcgg cgccgacggc tcattcacga
    17581 agacgatcgc tcgtcagcac gatctcgaac gaggcaccgc caccgcgcag gacttcggag
    17641 cgggctcgat gccgggggag ggcgtggttg ccccgcgtcc cgggttcgcc tccgaacagg
    17701 atgcttggat tttgcagctg gtgcatcgga aggagggcgc cgagacggcg ctcgtgatcc
    17761 tgaatgcggc ggactatgaa gcgcccccgg tcgcgagcat tcgaattccg ggtcgtgttc
    17821 cgtacggact tcacgccgag tggttgcccg atttggcgtg agcgtctccg tgaacgtctc
    17881 cacctgggcg caggtgggag cgagttgact cccgcgctca tgcgttgcat ggcaccgctc
    17941 tagagcatga aacctcgggt ccacaacctg gtcagctact gtggggcgtt cctcgtgttt
    18001 gcgctggtgc tcgggtggcg gtggtccacg cggcaggcct cctggcaacc ctcgctcgcg
    18061 ctcagcgccg gcgccagcat gtggcttctc catttcgcgc ggcgagccgc ggagtcggcc
    18121 tgggtccacc gctacgccaa acccggcgtc ccgcttgggg atgtgttggt cgagtacgtg
    18181 tactactggg gctttgcgct ctggaacgcc ctcagcctga gctcgcccaa ctatcacggg
    18241 cccacttccg cagtagtgct ctcggcggct ctggtgttcg gcctggcgga gctcggcaac
    18301 gcgcgggcgc atcgtatgct gcgagatctg cgcgcgcctg gcagcgcggc gcgcgtggtg
    18361 ccgcgaggtt ttctgttcga gcacgtttcg tgtccgcact acttgtgcga aatactctcg
    18421 tgggttggct tcgcgctcgc tacgcagacc tgggcggcgc tcggctttct gctcgttggc
    18481 tcggggatcc tcgcggcctg ggcgcgcacc cgtcacctgg cgtacctcaa ggagttcgac
    18541 ggcagcgagg ggcgtgaatt gtatccctcg acgcggcgtg ccctgatccc cggcatattc
    18601 tgagttggga ggccgctcgc ggggcgggag aggcgggtgc gttcgtgcta gcgatgggca
    18661 cgtcggtcgt aggctcgagg acgtctcggg gagcctttgg gcgcctgccc ggctgcctct
    18721 cggcacggga ctcgatcccc gtgtgatccg ccttgtgcct ccatcgggca gctgacagac
    18781 tgccgcccgg aacatgtccg taaagtctgc tcccaaatac cgtgacgtat cgcccgagct
    18841 cgatttgccg gggctcgagc accgcatcct gaagttctgg gaagagaatc gctgcttcca
    18901 gctgctccgc gagcaaaatg caggcaagcc gccgtggtca tttctggatg gcccgatcac
    18961 cgcgaacaac ccgatgggcg tgcaccacgc ctggggccgt tcgtacaagg acatgttctg
    19021 tcgcttccac gccatgcaag ggcgtgagct ccgctaccaa aatggcttcg attgccaggg
    19081 gctgtgggtg gaagtcgagg tcgagaagga gctcggcttc aagtccaagc gcgatatcga
    19141 acgctacggc attgccgaat tcgtcgagcg ttgtaaggag cgtgcgcgcc gcttcgccgc
    19201 cgtgcagacg gcccagtcca tccgtctcgg ttactggatg gactgggaaa actcgtacta
    19261 cacgatgtcg gacgagaaca attacaccat ctgggcgttt ctcaaacgct gccacgaaca
    19321 cggctgggtg tacaaaggcc gcgacgtcat gccctggtgt gcccgctgcg ggaccggcct
    19381 gtcgcaacac gagattgcca ccgaggggta ccgcgagctc acccacgttt cgccgtacgt
    19441 ccgcttccca ctcaagggtc gcgagcgtga gtaccttctg gtgtggacga ccacgccttg
    19501 gaccctgtct tcgaacgtcg cggccgcggt tcacccgaac aagcgttatt cgaaggtcaa
    19561 gcagggcgac gcgatctact acttgatcga gagccgtgag cacgttctga aggagcgcgg
    19621 tgcgttcgag cgcgtcgagt cattgctcgg ttcggagctc gtcgggctgg agtacgacgg
    19681 tcccttcgac gaactcgagg cgcagaacgg ggtaaagcat cgggtggtgg cgtgggaaga
    19741 agtcaccgat caggatggta ccggcatcgt ccacatcgct cccggctgcg gcaaagaaga
    19801 cttcgaactc tcgaaacacg aaggtttggc cgtgatcgcg ccgctcgatg agtacggtgt
    19861 attcggtccc gccttcgggc ccttcgccaa ccgcgaagcg agcaccatcc tcgatgacgt
    19921 actggcgaac ctcaaggcca aggggctcct gtacaagcaa gagcgctata cacactccta
    19981 tccggtgtgt tggcggtgtg gcagcgagct cttgttccgg ctcgtagacg agtggtacat
    20041 ccggatggga gagcagctcg ataagccgga tacggaactc acggccgagg agaaggcctc
    20101 gaatctgaga tatcaaatca tggattcggc gcgccaggcg acatggattc cgagctacgg
    20161 gctcgaccgc gagatcgact ggttgcgcaa tatgcatgac tggatgatct cgaagaagcg
    20221 ctattggggc ttggcgctac ccatcttcga atgctcgagc tgcggcaaat tcgacgtgat
    20281 cggcggccgc gaggagctgc gggagcgcgc cgtcagcggc tggtcggagt tcgaaggtaa
    20341 ctcaccccat cgcccgtggg tggatggcgt ggtgatctct tgcaagcact gcggcggcca
    20401 cgccactcgc atcaaggacg tgggcaaccc ttggttggac gcgggcatcg tcgggttctc
    20461 gaccatgcaa tggcgcgatg acccggagta ctggaagaag tggtatccgg ccgagctcgt
    20521 gaccgagagc ttcccggggc agttccgtaa ttggttctac tccttgctcg cgatgggcac
    20581 ggtgctgagc ggacaggctc cgttccgcag gttgttcggc tatgcgctaa tgaaggcgca
    20641 agacggccgc gaaatgcaca agagctgggg caactcgatc gagttcaacg aggccgcgga
    20701 caaggccggc gtggacgcga tgcgctggct gttcgcctcg cacattccgg atcaaaatct
    20761 gctcttcggg tacgacgccg tcgatgaggc ccggcgcaag ttcctcacgc tgtggaacgt
    20821 gtatagcttc ttcgtcactt acgcgaagat cgacggcttc gatccgcaga gcgcgccggt
    20881 gccgttggcg cagcgcccgg ccttggatcg ctggatcttg ggcggcctgc agcgcctcgt
    20941 gacggtcgcg cacagcgcgt gggagcagta tcagctccac accttcatgc gtcagctcga
    21001 gaagttcatc gacgagcttt cgacttggta cgtgcggcga agccgccggc gtttctggaa
    21061 gagcgagacc ggagcggaca agctcgcggc gtatcagacg ctccacgaag tgctggtaac
    21121 ggtgacggag ctgctcgctc cgatcacacc cttcgtgagc gaggaaatgt accagaacct
    21181 ggttcgttct tgggacgagt cggcacccgt cagcgtccat catcggagct tcccatcggc
    21241 tcgctccgag ctggtggacg cgaagctggc cgaagacatg gcggccgtgc tgcagatcgt
    21301 ggagctcggg cgccgagccc gtcaaaacgc ggcaatcaag gttcggcagc cgctcgccaa
    21361 gatggcgatc tcgctgcccg cggagatgga acagtcgcgg ctcgccgagt tcgagagcca
    21421 tatcctcgaa gagctgaata tcaaagccgt cgagtatatg ggcaacatgt ccccgtacct
    21481 acggcgcagc ttgaagctcg acgcgcgcaa agcaggcccg cggctcggag cggcaaccca
    21541 gagcgtactc gccgccgtac gcaagctgcc gccggaacaa ctcgcgagtc acacgcccgg
    21601 agcccccttg gaggtgaccg cgaacggcgt cacgtacacg ctcacggcgg aggagctcgt
    21661 gctcgacgag gctgcggtcg ccggcttcgc gtacgcaggg aatggtcggg tttcggtgct
    21721 cctgaccacg gagatcacgg aggacctgcg gcgtgagggc gcggtgcggg acgtgatccg
    21781 gctggtgcaa gagatgcgta agaaccacaa gctcgaagtg gcagaccgaa tttcgctcga
    21841 gctcgaaggt agcgaggcga tgcgcaacgc ggtgcaagcc cagctcgagc tgctcaaacg
    21901 cgagacgctt gcggtcgagg tgaatttccg cgcaacc
//
LOCUS       DASTKU010000446         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1646270, whole genome shotgun sequence.
ACCESSION   DASTKU010000446 DASTKU010000000
VERSION     DASTKU010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646270"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..397)
                     /locus_tag="VFQ61_15330"
     CDS             complement(53..397)
                     /locus_tag="VFQ61_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955881.1"
                     /translation="MEPTNAAEMVLLQIKVDAAKSRLEKAQVDLTSILNLLSDLPAAL
                     KTGVMRVIETAFEDLGEAEKHVLELKDIVRARATTFETVAPSDRCPHCSRPYKEATSS
                     SRGKDIGPAPQT"
     gene            complement(823..951)
                     /locus_tag="VFQ61_15335"
     CDS             complement(823..951)
                     /locus_tag="VFQ61_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955882.1"
                     /translation="MSKILRGVAAGWGAKKLGGGCLSTIIVFVILWYLLGHFQIFR"
     gene            complement(1111..1350)
                     /locus_tag="VFQ61_15340"
     CDS             complement(1111..1350)
                     /locus_tag="VFQ61_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955883.1"
                     /translation="MERAWKLRARPTDLCGNPTYRALDALWLAARPDHRDGFRRAVKA
                     SEASLPLVRDRRLTATVVPNDRSIACQQPHHFEFV"
     gene            1486..>2508
                     /locus_tag="VFQ61_15345"
     CDS             1486..>2508
                     /locus_tag="VFQ61_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955884.1"
                     /translation="MRRSVLLVGLLASTVACESSSGDGAGGQGGATTGGSSGGIASMG
                     GTASGGSATKGGASNGGTASSGASSGGTSTGGNSSGGMSVTSAGGASSGGASSGGVQG
                     ALGGREAGTGGRQTSSGGTTSQAGAGGKVGGQSGGAAGGSGSSTSCVGKAWTTADPTK
                     AGPFQVVADKNVGPLAGVVPDPIYGDKQQRFNVYRPSNLEQSGYCHPILIWANGYRDN
                     PEQNPPDCVVNSSSNQWCGQYSPMLKHLASHGFVVVASLSTATAEGNPLPTIAGLDWI
                     LKQSEDSSSPYYHHLDTANIGQLGHSYGAMSTCMSASEPRYKALAAICGTRALSGVHT
                     PMLFFCG"
BASE COUNT          488 a          779 c          804 g          437 t
ORIGIN      
        1 aaaaccatcc ggctcgagtc gggaggcaga tggcggtgga ttccgcgctg tgtcaggtct
       61 gaggagccgg gccgatgtcc ttacctcggg agctggaggt cgcctccttg tagggcctcg
      121 agcaatgggg gcagcgatcg cttggcgcga cggtttcgaa cgtcgtagct ctggcgcgca
      181 cgatatcttt caactcgagc acgtgttttt ccgcctcccc caggtcttcg aaagctgtct
      241 cgatgacacg catgacgccc gtcttcaacg ctgccggcag gtcgctcagc aggttgagaa
      301 tcgacgtcag gtctacctgg gctttttcaa gccgggactt cgctgcatct acctttattt
      361 gaaggaggac catttccgca gcatttgtag gttccatcgc gcctttccgg ccggggaaac
      421 tacaacgatc aacggagcag cttttccgac ctgcagaagg tagcacgctt tgcggtgccg
      481 agctcgggcc catgcatgac gcggcccatc gccccccgga acgcgccgcg gcctccgtgc
      541 tagttgaagc cgccaagctc gaacgcagcg gtcgccctcg ccaagcagta ccgagcatcg
      601 aattcggcac ggagatcgag atgcgagtcg tcaggcccac tgcatgcagc agtcctgaag
      661 agagcacgcg cgcccgatgc ggtctccgcg cggcgtgact cgacgaaggt agcggcgaaa
      721 agagtcaggc gcgccgccac atgaggcgat cgcgcttccc ctcatctgcc gcggtcaact
      781 tggtaggctg cgagaccgat cgtcgcgcgc gcccgcgccg cttcaacgga agatctgaaa
      841 gtggccgagt aggtaccaga gaatcacgaa gacgatgatg gtgctcaagc agccgccgcc
      901 gagctttttc gcgccccaac cagccgctac acctcgcagg atcttgctca tgacgcgtgc
      961 tcaagcgaag ggcgtgccac cgcagagcat ccagcgtgat ctatccaaag ttgatcgaag
     1021 tcacctcccg tggtcgacca cagatcatct cgaaattgaa atggctgccc gagaccggct
     1081 cgattgcctt ccgtcgaatg cttccccaag tcagacgaac tcgaagtgat ggggctgctg
     1141 acacgcaatc gagcggtcgt tgggcacgac cgtcgccgtg agccgccggt cacgaacgag
     1201 cggcagcgag gcctcggaag ctttgacggc gcggcggaat ccatcgcgat gatccggccg
     1261 tgcagcaagc caaagcgcat cgagcgctct gtacgtcgga ttgccgcaga ggtccgtcgg
     1321 gcgcgctcgc aacttccagg cgcgctccaa gttttctcca atgtttgagt gaatcagagc
     1381 actcgtcatc gcgggtgcgc ttttgcccgc taccgcagga cggattgaac ggcgtgaagt
     1441 cactgccctc gagctgctga tgcgagccga aagggaagca caacaatgag gcgttcagtc
     1501 ctgctcgtcg gcctattggc gtcgacggtc gcttgtgaga gttcatcggg tgacggcgcc
     1561 ggagggcaag gtggcgcgac gaccggaggt tcctcgggag ggattgcgtc catgggtggc
     1621 accgcgtccg gcggaagcgc cacgaagggc ggcgcgtcga acggcggaac agcctccagc
     1681 ggcgcgtcga gcggcggaac atccactggc ggaaactcga gcggtgggat gtccgtaaca
     1741 tccgcgggcg gggcctccag cggcggagct tcgagcggag gggtgcaagg tgctctcggt
     1801 ggtcgagaag ctggaacggg cgggcgtcaa acgagcagcg gagggaccac gagccaagcg
     1861 ggagctggag gaaaagtcgg cggacagagt gggggcgctg cgggcggcag cggttcatcg
     1921 acttcgtgcg tcggcaaggc ttggaccacc gcggatccaa cgaaggcggg cccgttccag
     1981 gtcgttgccg acaagaacgt ggggccccta gccggcgttg tccccgaccc aatctacgga
     2041 gacaagcagc aacgcttcaa cgtctaccgc cccagcaacc tggagcagag cggctactgc
     2101 cacccaatcc tgatttgggc gaacgggtac cgggacaacc ccgaacagaa tccccccgac
     2161 tgtgtcgtca actcgtcgtc gaaccaatgg tgtgggcagt attcgcccat gctgaagcac
     2221 cttgcgtctc acgggttcgt ggtggttgct tcgctcagca ccgccactgc agagggcaac
     2281 ccgctcccca cgattgcagg gctcgactgg atcctcaagc aatcggagga ctcgagcagc
     2341 ccgtactatc accacctgga cacggccaat atcggccagc tcggccattc gtacggcgcg
     2401 atgtccacgt gcatgtctgc gtccgagcct cgctacaaag ccctcgcagc aatctgcggc
     2461 acgagagccc tgagcggtgt tcacacgccc atgttgttct tctgcgga
//
LOCUS       DASTKU010000447         4650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1870038, whole genome shotgun sequence.
ACCESSION   DASTKU010000447 DASTKU010000000
VERSION     DASTKU010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870038"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1849
                     /gene="menD"
                     /locus_tag="VFQ61_15350"
     CDS             89..1849
                     /gene="menD"
                     /locus_tag="VFQ61_15350"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HET9955885.1"
                     /translation="MTDATLTRWAQLLLSTLARAGVRDVVISPGSRSTPFAWAALNTA
                     GLVCHSVIDERSAGFFALGQARVSGRPSLLLCTSGSAAAQYFPAVAEASYAHLPLIVM
                     TADRPFEAMDVGAAQTMDQTKLYGAFVRHAFELGHPEVSASALDGLERMVLQAVHTAL
                     EPLPGPVHLNARARKPLEPVAEEPDRAFRERLTRPVSRFWASRKVPHVGAIEQLAGRV
                     REARRGLLLVGPSPVYDRERVSALLELAEITGFPLCAETTSQLRHTEASANVRRVGGF
                     EVLLQNGSPELEPDFVLCFGAPPTTLAFSRLFAQRGVPYAVVTAQALPDPDNRASAVV
                     WGEPSEVARQLARTLRDASSLCSEQSSFSARWQRADNAYFRCVNELLEAESSEVLSEA
                     AAVRDVLSALPSGALLGLGNSLPVRDVDSFVAPSSRALRVWSQRGLNGIDGLVSGAAG
                     ASSAFGRSSLLLLGDVSFAHDLGGLACTRGLAQPLVICVIDNAGGRIFQELPIAKSFV
                     SQPELEHFWLTPPRLDLEHAAATFGLQYSEAETSATLRAGLEKALQEPGATVVRARVI
                     ADSARCLRERLRARISSSIE"
     gene            1856..2599
                     /locus_tag="VFQ61_15355"
     CDS             1856..2599
                     /locus_tag="VFQ61_15355"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9955886.1"
                     /translation="MSSIVALHGFTGTPSSFAPLAEYFGSANLLTPVSTGHGVPAQGS
                     EVASFEAEVSRLARLARDNSEGPVLVGYSLGGRLALGIALQFAASFRGLVLISASAGL
                     ESEAARAERRANDARWIALLEEHGVEAFADRWQAQPLFATQLELSQSLIEAERHRRLS
                     HTARGLAHSLRVTGLAEMPNYWPRLSEITLPVTLLAGARDPKFCASARRMASQIPGAV
                     VTEVPGVGHNLLLERPNAVIRAIEKGLLS"
     gene            2649..3647
                     /locus_tag="VFQ61_15360"
     CDS             2649..3647
                     /locus_tag="VFQ61_15360"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:NF004751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HET9955887.1"
                     /translation="MQLNLRAPAAAMSIALGSGSAPLARGSLRAWLMAARPATLTAAL
                     SPVIVGSACALHGSNFRVGAALGAMLGATLLQIGANFANDVFDFEKGADTAERLGPTR
                     AVQAGLVTPREMRIGMAVVFGLALMCGAYLTAVAGPWIVVIGLLSIACAVAYTGGPYP
                     LGYHGLGDVFVFIFFGPVAVVGSAFVHARELSGLALAASIPVGALTTAILVVNNLRDH
                     ATDLRAGKRTLAVRFGLRFTLIEYFALLVFAYLVPVVLCFWPERFALQPASAGNARLW
                     LLLPLFSLPVAALTFRAVRASSGRALNPLLGKTARLLFFFSALFAAGLVLSLSPSP"
     gene            3644..>4650
                     /locus_tag="VFQ61_15365"
     CDS             3644..>4650
                     /locus_tag="VFQ61_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9955888.1"
                     /translation="MSETTVELSALKRAARAPDACALITPELTLTYIALAECIEHCAN
                     ALFQAGHLPSASEEPLQPLAVVARPTLASLVLLHTLIAYQVPFALLHPRLPTQEREQQ
                     ARELGARVILEPDEHPLAAGIRRAPPPPEKERPGRVLAIVATSGSSGSPKWIALTRAA
                     FQSSALASAANLPLSDSDRWLLCLPLSHIGGLSIVTRCLLAGCAVVTFDPTPGGLLSR
                     LPQLTSWIERSRCSVVSWVPAVLDAWLERRDLSLPKSVRAVLLGGAACSERLLGRAVE
                     RGVPVLTTYGLTEACSQVTTTSYGTVPIVEQGQVSSGQALPGVELWIDSEQHICVRGP
                     ML"
BASE COUNT          676 a         1455 c         1584 g          935 t
ORIGIN      
        1 gaatcgtgga aggctcgaaa cccgagagtg agctgcaaga gacgcgctgg aagctacagg
       61 ggttattggg tgcgctcgga gttcctgcgt gacggacgcc acgctgacgc gttgggcgca
      121 gctgttgctc tcgacgctcg cacgcgcggg cgtgcgagac gtggtgatta gcccgggttc
      181 gcgttccacg ccgtttgcgt gggcggcgct gaacacagcg ggcctcgtgt gccactcggt
      241 gatcgacgag cgatccgcgg gcttctttgc gctggggcaa gcgcgcgtca gcgggcggcc
      301 gagtctactg ctctgtacgt ccggttcggc tgcggcgcag tacttccccg ccgttgccga
      361 ggcgagctac gcgcatctgc cgctgatcgt gatgacagcg gaccgtccat tcgaggcgat
      421 ggacgtgggt gccgcgcaga ccatggatca aaccaagctc tacggcgcgt tcgtccggca
      481 cgcgttcgag ctgggacacc ctgaagtttc agcgtctgcg ctcgacggct tggagcgcat
      541 ggtgctgcag gccgttcaca ccgcgctcga gccgctgccc gggccagtgc atctgaacgc
      601 gcgcgctcga aagccgctcg aacccgtcgc ggaggagccg gaccgcgcgt ttcgcgagcg
      661 gctgacccgg cctgtttctc gcttttgggc cagccgaaaa gtgccacacg tgggtgccat
      721 cgagcagttg gcgggccgcg ttcgcgaggc tcgtcgcggg cttttactgg tggggccctc
      781 accggtttac gatcgtgaac gagtaagtgc gctgctcgag ctggccgaaa taacgggatt
      841 cccgctttgc gccgagacca cgagccagct gcgacacacg gaagcttcag ccaacgtgcg
      901 gcgggtgggt ggcttcgaag tcctgctgca aaatgggtcc cccgagctcg agcctgactt
      961 cgtgctgtgc ttcggggcgc cgccgaccac gttggcgttt agccgtctgt ttgcgcagcg
     1021 cggggtgcca tacgcggttg tgactgcgca ggccttacca gatccagaca atcgagcgag
     1081 cgcggtggtc tggggcgagc cgagcgaagt ggcgcgacag ctggcacgca cgttgcgaga
     1141 cgcttcgtcc ctgtgctctg agcagagctc cttctccgct cgttggcagc gcgccgacaa
     1201 tgcatacttt cgctgcgtta acgagctact cgaagccgag tcgtccgagg tcctgagtga
     1261 ggcagccgcg gtgcgggacg tgctgagcgc gctaccgagt ggggccctac tcggcctcgg
     1321 taacagcctt cccgtgcgtg atgtcgacag cttcgtggcc ccaagctcgc gtgcgctccg
     1381 agtgtggtcg cagcgcggct tgaacggaat cgacggtctt gtgtcggggg cggccggggc
     1441 atcgtcggcc ttcggtcggt ccagcctgct gctcttgggc gatgtcagct tcgctcatga
     1501 cttgggtggg ctggcgtgca cgcgtggcct ggcccagccg ctggtcatct gcgtgatcga
     1561 caacgcgggt ggccgtatct ttcaggagtt gccaatcgcg aagagcttcg tgtcgcagcc
     1621 ggagctcgag cacttttggc tgaccccgcc gaggctagat ttggagcacg cggccgcgac
     1681 gtttggcttg caatacagcg aagccgagac ctctgcgacc ctgcgggcgg ggttggaaaa
     1741 ggcgctgcaa gagccgggag ctacggtagt gcgcgcgcgc gtcatagcgg acagcgcgcg
     1801 ctgcttgcgc gaacgcctgc gagcccggat ttcgagctcg atcgagtgac gaaccatgtc
     1861 gtcgatcgtt gctcttcacg gctttaccgg gacacccagc tcgtttgccc cacttgccga
     1921 gtactttggc tcagcgaacc tgctgactcc ggtgtcgacc ggacacggcg ttccggcgca
     1981 aggcagcgaa gttgcctcgt tcgaagccga agtctcgcgt ctagccaggc tggcgcggga
     2041 taactcggaa gggccagtgc tcgtgggcta ttcgctcggt gggcgtttgg cgcttgggat
     2101 cgcgctgcag ttcgcagcgt cgtttcgcgg cctggttctg atttctgcgt cagcggggct
     2161 cgagagcgaa gcggcgcggg ccgagcgccg cgccaacgac gcgcgttgga tcgcgctgct
     2221 cgaagagcac ggcgtagagg cgttcgcgga tcgttggcag gctcaacctc tgttcgcgac
     2281 ccagctcgag ctgtctcaat ccttgattga agccgagcgt caccgccggc tttcgcatac
     2341 cgcccgaggg ctggctcatt cgttgcgcgt gaccggactt gcggaaatgc ccaattactg
     2401 gccgcgccta agcgaaatca cactccccgt cacgctactc gctggcgccc gcgaccccaa
     2461 gttttgcgct tcggcgcgca gaatggcctc gcagattccg ggcgcggtgg tcaccgaagt
     2521 cccaggagtg gggcacaatc tgctgctaga acggccgaac gccgtgattc gagcaatcga
     2581 gaaagggctg ctttcatgag cgacgtgcga tgcaaagggc gcgagggcca cgacgccttc
     2641 atcgagaagt gcaactcaac ctccgagccc cagcggcggc catgagtatc gcgctcggtt
     2701 ctggctcggc tccgctggcg cggggctcgt tgcgcgcctg gctgatggcg gctcggcccg
     2761 caactctgac ggccgccctt tcgcccgtga ttgtgggcag tgcgtgcgcg ctgcacggat
     2821 ccaatttccg ggttggggcg gcgctcgggg ccatgctggg agccacgctg cttcagattg
     2881 gcgcgaactt cgcgaacgat gtgttcgatt tcgaaaaagg agcggacacg gccgagcgcc
     2941 tgggccccac gcgcgccgta caggccggcc tcgtcacgcc gcgcgagatg cggattggga
     3001 tggcggtcgt gttcggcctg gccttgatgt gtggcgcgta tctgacggcg gtggcggggc
     3061 cctggatcgt ggtcatcggc ttgctctcga ttgcctgtgc ggtggcgtat accggtggcc
     3121 cctacccgtt gggttatcac ggactcggcg atgtattcgt gttcattttt ttcgggcccg
     3181 ttgcggtggt gggcagcgcg ttcgtgcatg ctcgagaact gagcggcttg gcattggctg
     3241 cctcgatacc ggtcggagcg ctgacgaccg cgatcttggt cgtcaacaat ctgcgcgatc
     3301 acgcgacgga tctgcgggca ggaaagcgca ccttggccgt ccgcttcggg cttcggttca
     3361 ccctgatcga gtactttgcc ctgctcgtgt tcgcgtactt ggttccggtg gtgctttgct
     3421 tctggcccga gcggttcgcg cttcagcccg cgagcgccgg aaacgctcgg ctttggctgt
     3481 tgctgccgtt gttcagcttg cccgtggcgg cgttgacgtt tcgcgccgtc cgcgcaagct
     3541 ctgggcgggc gctcaatccg ttgctcggga agactgcccg tttgctgttc ttctttagcg
     3601 cgttgttcgc ggctgggctg gttctttcgt tgtcgccgtc gccgtgagcg agactaccgt
     3661 ggagctcagt gccctcaaac gagcagcgcg ggcgccggac gcctgtgcgc tcatcactcc
     3721 ggagttgacg ctcacttaca tcgcgctcgc ggagtgcatc gagcactgcg ccaacgcgct
     3781 ctttcaagct ggtcatctcc cctccgcgag tgaggaaccg ctccaacccc tcgccgtagt
     3841 ggcgcgacct acgttggcca gtctcgtgct cttgcacacg ctgattgcct accaggtgcc
     3901 gttcgcgctc ctccacccgc ggctgcctac gcaggagcga gagcagcaag cacgcgagct
     3961 cggcgcgcgc gtcattctag agcctgacga acacccgctg gcggcaggca tacgtcgcgc
     4021 accaccgcct ccggagaaag agcgccccgg gcgcgtgttg gccatcgtgg caacctcggg
     4081 tagcagcggc tcgccaaagt ggattgcctt gactcgggct gcgttccagt cgtctgcgct
     4141 cgcgagcgcg gcgaacttgc ccttgagcga ctcggatcgt tggctcttgt gccttccgct
     4201 gtcgcatata ggcgggcttt ccatcgtgac tcggtgtttg ttggcgggct gcgccgtggt
     4261 cacgttcgat ccgactccgg gcgggctgct gtcgcggctt ccgcaactca cgagctggat
     4321 cgagcgcagc cggtgcagcg tggtatcttg ggtgcccgcg gtgctcgacg cctggctcga
     4381 acgacgcgat ctctcacttc cgaaaagcgt acgtgcggtt ctgctcggcg gtgccgcctg
     4441 ttcggaacga ttgctcgggc gggccgtgga acgcggcgtg ccggtactaa cgacatacgg
     4501 ccttaccgaa gcctgttcgc aggtcacgac caccagctac gggaccgttc cgatcgtcga
     4561 gcaggggcaa gtcagctccg gccaggccct accgggtgtc gagctctgga tcgattccga
     4621 gcagcatatt tgcgtgcgcg gtcccatgct
//
LOCUS       DASTKU010000448         8269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_84938,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000448 DASTKU010000000
VERSION     DASTKU010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84938"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="VFQ61_15370"
     CDS             complement(<1..174)
                     /locus_tag="VFQ61_15370"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HET9955889.1"
                     /translation="MSKPPISSSPVDQQLPETTSLLELALLFVRLGSTAFGGPAAHIA
                     MMQDEVVTRRKWLT"
     gene            complement(294..713)
                     /locus_tag="VFQ61_15375"
     CDS             complement(294..713)
                     /locus_tag="VFQ61_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9955890.1"
                     /translation="MSGSGIIRVEHFYSHPPAAVWRALTDPALHARWFVKGDVRAEVG
                     HRFELDMGKWGKQPCEVLVAEPERVFQYRFAADTLNTIITWRLTPEGTGTRLEMTQEG
                     FDLDSPFGRQALEGMKPGWPRILERLPGALQEASPNL"
     gene            complement(710..1063)
                     /locus_tag="VFQ61_15380"
     CDS             complement(710..1063)
                     /locus_tag="VFQ61_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9955891.1"
                     /translation="MTRRKDPTYCPDMLEVDVFAALANPVRREILVRLRRGPRAVSDL
                     AEGFSIGRPAVSEHLQVLRKAKLVREEARGRERYYHLDPRPLGEVETWLAAFSRYWQQ
                     RLNDLDQLLNEEQKK"
     gene            complement(1145..3403)
                     /locus_tag="VFQ61_15385"
     CDS             complement(1145..3403)
                     /locus_tag="VFQ61_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HET9955892.1"
                     /translation="MTWTTPWKALIVILVASFAVLLYAGHRTAAEAPPIPSTVRSQSG
                     EVLIAEEEILDGQNVWQAMGGMELGSVWGHGSYVAPDWTADYLHREARSMLDQYAAER
                     GAANYDALDVETRAALRARLQQVVRSNRVDANGALIVSEERARAFKAIQAHYAELFSQ
                     GRRELALPAGAQSDPHKQHLLTSFFFWSAWAAATDRPNSPGVSFTNNWPHEPLVANTV
                     TGDAVVWTGVSIIVLLAGLSAMVWWHGARSPAASGHVPPPDDPLLGARLTPSQKAALI
                     YFGVVIVLFMLQIGMGVVTAHFGVEGDGFYGIPLSRWLPYVISRTWHTQLGIFWIATA
                     WLAAGLFVAPAIGAGDPPYQRLGVHVLLGALVVVVLGSMAGQWASVMHRFSDDTTRFY
                     LGHSGYEYIDLGRAFQIALLIGLFLWVTLVLRAVWPALMRKDEQRGLLVLFTLSALAI
                     AGFYGAALGYGHRTHLSIAEYWRWWVVHLWVEGFFEVFATVVFAFLFARLGLLEPKST
                     ERAVVASSAIFLTGGIIGTLHHLYYSGTPQLITALGAVFSALEVVPLVFIGYEAWENL
                     RLSRHNGWVQKYRYPIYFFVAVAFWNAVGAGLFGFMINPPVALYFMQGLNTTPVHGHA
                     ALFGVYGMLGLALTLFCARAADAEREWDERPLAASFWLLNIGLLAMVVISLLPVGLLQ
                     TRAAVQVGYWYARSPEFLQTPIMNALRWSRVIGDTLFALGALAFAWFAVSLLRSRKAA
                     APTLARPAEAAE"
     gene            3785..4114
                     /locus_tag="VFQ61_15390"
     CDS             3785..4114
                     /locus_tag="VFQ61_15390"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9955893.1"
                     /translation="MLQTFAALSEPTRLRIFEYLGEQPRSVSEICDSLSLTQPQVSKH
                     LRVLRESGVVDVEARAQSRIYSVRAEPLRELQLWLDRYRSIWDERLDVLAELAQEIDH
                     KRKKPRR"
     gene            4117..4614
                     /locus_tag="VFQ61_15395"
     CDS             4117..4614
                     /locus_tag="VFQ61_15395"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9955894.1"
                     /translation="MSTNSTTLERTSDRELIITRTFRASASAVFQAWTEPEYVKRWWA
                     PRSRGVSVAECTARVEPGGNYRYVLALPNGEQIAFSGKYIEIQAPTRLVYTQRFEAVP
                     AGEALLTVLFEEQSGLTRMVSREVYPSKEALEMALTSGMEDGMRETFDQLDMLLSSAA
                     SAPAA"
     gene            4707..5204
                     /locus_tag="VFQ61_15400"
     CDS             4707..5204
                     /locus_tag="VFQ61_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9955895.1"
                     /translation="MPSPTDRIEKKVVLRASRERVWRAISESREFGSWFGVDFDGPFV
                     AGQPLKGRIVPTRADPEIAKSQQPYAGTPFEISVTQLEPLRCMAFRWHPYAVDEGQDY
                     SKEPTTLVTFELEDAAEGGTQLTITESGFDSVPLERRAKAFSQNEQGWTIQTSLISKY
                     LAAHA"
     gene            5286..5663
                     /locus_tag="VFQ61_15405"
     CDS             5286..5663
                     /locus_tag="VFQ61_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9955896.1"
                     /translation="MPRSRSAAAQIADAAPLFAALGDETRLRLVTRLSAEGPQSIVKL
                     TDSASVSRQAITKHLQALAGAGLVRSRREGRERVWELEAKRLNDARRYLDQISAHWDS
                     AIDRLRALVEDKPPVDPAKPNRG"
     gene            complement(5687..8161)
                     /locus_tag="VFQ61_15410"
     CDS             complement(5687..8161)
                     /locus_tag="VFQ61_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9955897.1"
                     /translation="MTKNQLFLGIDLGTTNSVQAVHDGDTCKLVRSSDGSVLTPSVVR
                     IDGRGRVSVGARARSFLVSDPDNTRSEFKRLMGSPHGLDFGAAHVSRSPSELSAEVLK
                     SLRHDFAEQFGEQPMRAVISVPALFELPQLEATSDAARLAGFERVEFIQEPIASAIAS
                     GWSAQDGQNGSWLVYDLGGGTFDVSLLESREGLLRVVGHDGNNFLGGRDIDQGLAEWA
                     LGRLARDGAVLDVTRPELFSALRRLRAACELAKIELTRSHEADIVVPELCELGGAKIS
                     VDLVVTRAELEVIVRPIVERSLEICLRLLGAHGLAPEQLQHVVLVGGPTLIPSVRELL
                     AAVLPVSGANGVTLKSVDPMALVAEGAALFAFSQGLSSAPAAKTSVSAAGPSVWLQYS
                     AVSSDPNPYVVGKVGNAAKPMTTVCIERTDHGFSTQDTPLDDEQAFAVPVELIPRGRA
                     TFRIWGKAEDGVKVALVPHEFTISHGVSLGEPPLSRSIGVALANDEVQVFFEKGSPLP
                     VRRTFRLRTVETVSPGNTGFALLVPIVQGEFPFAHLCRLVGALEISAAQIATAVPAGA
                     AVEVTLELDRGGRLSASAKIDGLDQVFEQVAHLVMPDSSIDSMRLARNALSERGALLR
                     GEAFRNGDQRALRTLLEAERLNGDAQRAIELAAGGDPDASERARRLLIDLDGLLSEAE
                     ALRSWPKLEEEARYRLAVESGWLAKYGTTVEQSLFDKTAEALERALKAKSSSEVRRQL
                     HAAARLGDAAYYRSPRAWPDAFERLLTRVHELSNPVQGKKLVEEGRQALARGELRELE
                     RIVTGLWQLLPPDPERRRLGHDSGVR"
BASE COUNT         1676 a         2734 c         2451 g         1408 t
ORIGIN      
        1 ggtgagccac ttccgacgcg tcaccacctc gtcttgcatc atggcaatgt gcgccgcggg
       61 gcctccgaaa gcagtgctgc ccagacgcac gaacagcagc gccaattcca acaaggaggt
      121 cgtctccggg agctgctgat cgacaggact cgaagagatg ggaggcttgc tcaaggccgc
      181 ccatcttctc gtgacgcgcg tgccggcgcc acagattcat gcaccgtact ctgcgccgct
      241 ctctcctttt ggcgctcgtt cgcacccagg ggcgcggcgc ggcccgcgcc gcctcaaaga
      301 ttcggcgaag cttcttggag cgcccctggt agccgctcca ggatgcgagg ccaaccgggc
      361 ttcatgcctt ccaacgcctg acggccgaat ggagaatcga gatcgaaccc ttcctgagtc
      421 atttcgagcc gcgttccggt gccttccgga gtcaggcgcc aagtgatgat ggtgttgagc
      481 gtgtcagccg caaatcgata ctggaatacc cgctcgggtt ccgcgaccag cacctcgcag
      541 ggctgtttgc cccactttcc catgtccaac tcgaaacgat gacctacctc tgcgcgcaca
      601 tctcccttta cgaaccagcg agcgtgcagc gctgggtcag tcagtgctcg ccagaccgcc
      661 gcgggcggat gtgaatagaa gtgttcgact cgaatgatcc ctgaaccgct catttcttct
      721 gctcctcgtt caaaagctga tcgaggtcgt tgagccgttg ctgccaatag cgggagaacg
      781 ccgcgagcca ggtctcaacc tcgccgagag ggcgcggatc caagtggtaa tagcgttcac
      841 gcccccgcgc ctcttcacgc acgagcttgg ccttgcggag aacctggagg tgctccgaaa
      901 ccgcagggcg gccaatcgaa aagccttccg ccaaatcgct cacggcacga ggaccgcgcc
      961 gtaaacgcac caagatctcg cgacgtaccg gatttgcgag ggcagcaaag acgtcgacct
     1021 cgagcatgtc gggacaatac gtcggatctt tccgacgcgt caactttttc cgacgcttca
     1081 cgaacaaggg cgcgcggcct gtcgggaccg cgcgcctgag ctagggatcg gatctaactc
     1141 atcgtcactc ggcagcctcg gccggacgag cgagcgtcgg cgccgcggct ttcctggagc
     1201 gcagcaagct caccgcaaac caagcgaacg ccagcgcgcc cagcgcgaat agtgtgtctc
     1261 caatcacgcg cgaccagcgc agcgcattca tgatcggtgt ctgcagaaac tccgggctgc
     1321 gcgcgtacca ataccccact tgcactgcgg cccgcgtctg caacaaccct accggaagta
     1381 ggctgatcac gaccattgcc aacaggccaa tgttcagcag ccagaacgac gcggccagcg
     1441 gacgctcgtc ccactcacgc tctgcatcgg cggcgcgggc acagaacaag gtgagcgcca
     1501 ggcccaacat gccgtaaacg ccgaatagcg ccgcgtgacc gtgcaccggt gtcgtgttca
     1561 acccttgcat gaaatacaaa gcgaccggcg ggttgatcat gaacccgaac aagccggcgc
     1621 cgaccgcgtt ccagaacgca accgccacga agaagtagat cgggtaacgg tacttctgaa
     1681 cccagccgtt gtgtcgcgac aagcgcaggt tttcccaagc ttcgtagcca atgaatacca
     1741 acggcacgac ttcaagagcg ctgaacaccg cgccgagcgc cgtaatcagc tgcggcgtgc
     1801 cactgtaata caggtgatga agcgtgccga tgatgccgcc ggtcaagaag atcgcggagg
     1861 aggccacgac cgcgcgctcg gtcgacttcg gctctagcag cccgagccgc gcgaacagga
     1921 aggcgaacac cacggtcgcg aagacctcga agaagccctc cacccagaga tgcaccaccc
     1981 accaacgcca atactccgca atcgagaggt gcgttcggtg gccgtacccc aaagcagcgc
     2041 catagaagcc tgcgatcgcg agggcggaca acgtaaacag gaccaacaac ccgcgctgtt
     2101 cgtctttccg catcagagcc ggccataccg cgcgcaggac cagcgttacc cacaagaaca
     2161 acccaatcag tagcgcaatt tggaacgcgc gacccaaatc gatgtactca taaccgctgt
     2221 ggcccaagta gaaccgcgtc gtatcatcgg agaagcgatg catcacgctc gcccactgcc
     2281 ccgccatcga tccgagcacg accaccacta gcgcgccgag caatacgtga accccgagac
     2341 gctgataggg agggtcaccg gcaccaattg ccggcgccac gaacagcccc gccgcgagcc
     2401 acgcggtggc gatccagaag atccccagtt gggtatgcca ggtcctagag atcacgtaag
     2461 gcaaccagcg cgacagcgga atgccgtaga agccgtcccc ctccaccccg aagtgtgcag
     2521 tcactacgcc catgcctatt tgcagcatga acagcacaat cacgacgcca aagtagatta
     2581 gggccgcctt ctgtgaggga gtgagtcggg cgccgagcag cggatcgtcg ggcggcggca
     2641 catgtcccga agcggccggt gatcgagcgc cgtgccacca aaccatcgcg gagagaccgg
     2701 cgagcagaac gatgatgcta accccggtcc aaaccaccgc gtcacccgtg acagtgtttg
     2761 cgaccagcgg ttcgtgcggc caattattcg taaacgagac gcccggcgag ttcgggcgat
     2821 cggtggcggc ggcccaagcg gaccaaaaga agaagctcgt gagtaagtgt tgcttgtgcg
     2881 ggtccgactg cgcgcccgcc gggagcgcca gctcacgccg cccctggctg aagagctcgg
     2941 cgtagtgggc ttggatcgcc ttgaaggcgc gcgcccgctc ctcactcacg atcagcgcgc
     3001 cattggcgtc cacgcgattg gagcgcacca cttgctgaag cctggctcgg agcgcggctc
     3061 gggtttcgac atcgagcgcg tcgtaattgg cagcgccgcg ctccgccgcg tactgatcca
     3121 acatcgagcg agcttcacga tggaggtaat cggcggtcca gtcgggagcc acgtagctgc
     3181 cgtggcccca caccgagccg agttccatgc cgcccatcgc ctgccagaca ttctggccat
     3241 ccagaatttc ctcttcggcg atcaacacct cgccggattg gctacgaacg gtcgagggaa
     3301 tcgggggcgc ctcggccgcc gtgcgatgcc ccgcgtagag caacacagcg aacgaggcga
     3361 cgagaattac gatcagtgcc ttccacggag tcgtccaagt cattgcaacg cggccacagc
     3421 aagacccaga ccacccagca tatgccgatg tttcctgcat gcgattaaca gggcgcaggt
     3481 ctgggtgttc caaaatctaa cggctggaac acgcaccccc gcttgcaatc gacgtttgca
     3541 ttcgtgaact gcgctctaac agcggatgct gtggcgttat gccattcgga tgtcctcacg
     3601 attcaactct gactaacccg gcggatggtc gaacccggag cgccctgtcg agtcacgcct
     3661 tgtcggagtt ggttttcgca cccgaattcg tattccagcg cgagccctgc aaatatacgt
     3721 tgacgcgcat atacgccatc gggcatataa tgaggcatat acatcgtgga gagtgaacaa
     3781 cgccatgctg cagaccttcg cagcactcag tgagcccaca cgacttcgca tcttcgagta
     3841 cctcggggaa cagccccgtt cggtcagcga aatctgtgac tcgctttccc tcacccagcc
     3901 gcaagtctcc aagcacttga gagtgctgcg agagagcggc gtggtggatg tcgaagctcg
     3961 ggcccagagt cgcatttact cggtccgggc cgagccgctg cgagaattgc agctatggct
     4021 cgatcgctat cgatcgattt gggacgagcg cctcgatgta ttggccgagc tcgcccaaga
     4081 gatcgatcac aaacgcaaga agcccaggag atgatcatga gcaccaattc gacgaccctc
     4141 gagcgcacgt ccgatcgcga actaatcatt acacgcacgt tccgtgcgtc ggcttcggcc
     4201 gttttccagg cctggaccga gcccgagtac gtcaagcgtt ggtgggcgcc tcgctcccgg
     4261 ggtgtcagcg tagccgagtg tacggcgcgc gtcgaacccg gcggcaacta tcgatatgtg
     4321 ctcgcgttgc caaacggtga acagatcgcg ttttccggca agtatatcga gatccaagca
     4381 ccaacgcggc tggtctacac ccaaaggttc gaggccgtgc cggcgggtga agcgctcctg
     4441 acggtcctct tcgaggagca aagcgggctc acgcgcatgg tctcccgcga ggtgtacccc
     4501 tccaaagagg cgctcgagat ggcgctcacc tcgggtatgg aagacggaat gcgagagacg
     4561 ttcgatcaac tggatatgct gctctcgtcc gcggcgagcg cgccggccgc gtaagatcga
     4621 agttcgagac gccagcagca aacgcaacct ttcggttgca tcaaatcccg atcggggtta
     4681 gagcttcagc aaccaagagg ttgcacatgc ccagccccac cgaccgcatc gaaaagaagg
     4741 ttgtactgcg cgccagtcgc gagcgcgtct ggcgcgccat cagtgagtca cgagagtttg
     4801 gtagttggtt cggcgtcgac ttcgacgggc cattcgtagc gggacagccg ttgaagggtc
     4861 gtatcgtccc tacccgggcc gacccggaga tcgccaagtc tcagcagccc tacgcgggca
     4921 cacccttcga aatcagcgta acgcagctcg aacccttacg ctgcatggcg tttcgttggc
     4981 atccgtatgc ggttgacgaa ggtcaggact actccaagga acccaccacg ctcgtgacct
     5041 tcgaactgga agacgcggcc gaaggtggta cccagctcac catcacggag tcgggattcg
     5101 actcggttcc gctcgagcgt cgtgccaaag ccttcagcca gaacgagcag ggctggacca
     5161 tccaaacttc gctcatctcg aagtacctgg cggcgcacgc gtgactccgc cgacgctctc
     5221 aacccagcag agtaaccccg aaccgtccat acccggcccg ttctgagcta gggtgtcggg
     5281 gatccatgcc gcgctcccgc tccgctgccg cccagatcgc cgacgccgct ccgttgtttg
     5341 cggcgctcgg cgatgaaact cgtctgcgcc tcgtgactcg gctctctgcg gaggggccgc
     5401 agtccatcgt gaagctgacg gatagcgcgt ccgtttcgcg gcaagccatt acgaagcacc
     5461 ttcaggctct cgccggcgcg gggctcgttc gcagccggcg tgaaggtcgg gagcgcgtct
     5521 gggagctcga agccaagcgt ttgaacgatg cgcggcgcta cctcgaccaa atctcggctc
     5581 actgggatag cgccatcgat cggctgcggg cgctagtcga agacaagccc ccggtcgacc
     5641 cggccaagcc caaccgaggt tgagccgctt cagggccgac gtgcactcaa cgaactccgg
     5701 agtcgtgtcc gaggcggcgt cgttccgggt ccggtggcaa aagttgccaa aggcccgtta
     5761 cgattcgctc caattcccgc agctctccgc gtgcgagcgc ttgtcggcct tcttcgacta
     5821 gcttcttgcc ttggacgggg ttcgagagct catgcacgcg cgtgagcagc cgttcgaagg
     5881 cgtcgggcca cgctcgcgga cttcgatagt aggccgcatc cccgagccga gccgccgcgt
     5941 gcaactgacg gcgcacctcg gaagaactct tggccttcag tgcgcgctcg agagcttccg
     6001 cagtcttgtc gaacagactc tgttcgaccg tggtcccgta tttggcaagc cagccactct
     6061 cgacggccag gcgatagcgc gcttcttcct cgagtttcgg ccaggagcgc agagcctccg
     6121 cttccgagag tagcccgtcc aaatcgatca gcagccgacg tgcacgttcg ctcgcgtcgg
     6181 gatcaccgcc ggcagcgagc tcgatggcgc gctgcgcatc accgttgagg cgctctgctt
     6241 cgagcagggt gcgtaaggcc ctctgatcgc cattgcggaa agcctcgccg cgcagcaggg
     6301 ccccgcgctc gctcaaggcg ttgcgagcga gccgcatgct gtcgatgctc gaatccggca
     6361 tgaccaagtg tgcgacttgc tcgaacactt gatccagacc atcgatcttg gccgaggcgc
     6421 tcaaacgtcc gccgcgatcg agctcgagcg tgacttcgac cgctgccccc gccggcaccg
     6481 ccgtcgcgat ttgcgccgcc gagatctcca aggcaccgac gagccggcac aggtgagcaa
     6541 agggaaactc cccctgcacg atgggcacca acagcgcgaa cccggtattt cccggagata
     6601 ccgtctccac ggtgcggagc cgaaatgttc tcctgactgg caacgggctc cccttctcga
     6661 agaagacctg gacttcgtcg ttggcgagcg ccaccccgat cgagcgagac agcggaggct
     6721 cacctaggct cacgccgtgt gagatcgtga actcgtgagg tacgagggca accttcactc
     6781 catcctctgc tttgccccaa atccggaatg tcgcccgacc tctggggatc aactcgaccg
     6841 gcaccgcgaa ggcttgctca tcgtcgagcg gcgtatcctg cgtgctgaaa ccgtgatccg
     6901 ttcgttcgat gcaaaccgtg gtcattggct tggcagcgtt gcccaccttt ccgaccacgt
     6961 acggattcgg atcggagctc accgcggagt actgcaacca aacgctcggg ccagccgcag
     7021 agacgctcgt cttggcggcg ggagcgctgg aaaggccctg agagaaggcg aacagtgccg
     7081 cgccctccgc gaccagcgcc atcggatcca cggacttgag tgtgacgcca ttcgccccgc
     7141 tgacaggaag taccgccgcg agcagttccc gaacgctcgg aatcaaggtc ggacctccca
     7201 ccaacaccac gtgttgcagc tgctccgggg cgagcccatg ggctccgagc aggcgtaggc
     7261 aaatctccaa gctccgctcc acgatcggcc gcacgatgac ctcgagctcc gcgcgagtca
     7321 cgaccagatc gacgctaatc ttggcccctc cgagctcgca cagctcgggc acgacgatgt
     7381 ctgcctcgtg gcttctcgtg agctcgatct tcgccaattc gcaggcagcc cgcaggcgcc
     7441 gtagggccga gaacagttcg ggccgagtca cgtcgagcac cgcaccgtct cgagcgaggc
     7501 ggccgagcgc ccactccgca agcccctggt cgatgtcgcg cccacccaag aagttgttgc
     7561 catcgtgacc cacgacccgc aacagccctt cccgggactc cagaagcgaa acgtcgaacg
     7621 tgcccccgcc taggtcgtag accagccagc taccgttctg gccgtcctgc gcagaccagc
     7681 cagatgcgat ggctgacgcg atcggctctt gaatgaactc cacccgctcg aacccggcca
     7741 agcgcgcggc atcgctcgtg gcctcgagct gcgggagttc gaacaacgcg ggcacggaga
     7801 tgaccgcgcg catgggttgc tcgccgaact gctcggcaaa atcgtgccga agggacttga
     7861 gcacctccgc cgacagctcg gacggtgaac gagagacgtg cgcggctccg aagtcgagcc
     7921 catgcggcga gcccatcaaa cgtttgaact ccgagcgggt attgtccgga tcgctcacca
     7981 agaagctgcg ggcgcgcgcg cccacgctga ctcttccccg cccgtcgata cggacgacgc
     8041 tcggggtcaa aaccgagccg tcggagctac gcaccaactt gcaggtatcg ccgtcatgga
     8101 cggcctgcac cgagttcgtc gtccccagat cgattcccaa aaagagttgg ttcttggtca
     8161 tgaacgcgtg aaccagccta gcgcaaaatc gcgcgattca ctgtcacgtg ggctggcagc
     8221 gggcgttctt ggagcagagc tcgatggctt tttcgtcctg catccaaat
//
LOCUS       DASTKU010000449        13528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1424626, whole genome shotgun sequence.
ACCESSION   DASTKU010000449 DASTKU010000000
VERSION     DASTKU010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13528
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424626"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1728
                     /locus_tag="VFQ61_15415"
     CDS             <1..1728
                     /locus_tag="VFQ61_15415"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9955898.1"
                     /translation="IVQVFDSGIDNPTGRPYLVMELLRGEDLRTTLARTGALEPICAG
                     RLALQATLGLTRAHALGIFHRDIKPANLYLARRDSGEICVKLLDFGIAKVRAESGLFT
                     TETLTRTGGILGTPLYMSPEQIRGASDVDARSDVWSLGVVLFECLSGQLPFDGNGSIG
                     ELMAAILTTNARPLREIAPWVPDALVDVVTQALERDPARRFADASALGEALARVLPSG
                     VTVLESALVSARPLDQTRVSGEVPVVEAQMGLAAPSTPVGLSPRPIPDASSDAWRLVR
                     ARAETSDSDLASPILRRSAGNDGSGAAVPGSNSPSTAVIEPHANAPLTPAQEHCNPTW
                     LSGNPPPVQSSLRDPGPVSRTRWGLPIAFALGLLGAAITIVLWTTSRHTLVSVSSSEG
                     SALNSAAPYSPKTPPPQLPATATASVPTGAPQVELANDPPPVVAAYVEIQVPLGARVL
                     ADSQPLAVETRGSAGYVKLPSKPDRGVRLELTGVGPKVTELVQIQQGKAVPGVLTRRT
                     EASLQRTPKPSEPPAVPPAPRGVFFDLEEEPAPAPHPAQRQPNSAPRGSLNPPGSAAK
                     YPDPPPSSP"
     gene            1838..3391
                     /locus_tag="VFQ61_15420"
     CDS             1838..3391
                     /locus_tag="VFQ61_15420"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9955899.1"
                     /translation="MLRATLRSPANRIVPSRSALSRPGLNALVVLTALLTARHGSAQA
                     SVSSASANGTPAAANAEAPNPSDPSAREPDPARRNAAIEHFRRGLELARKGNCSAALP
                     EFLISRELSPLVPALINAAICLQDLGRPAEALDLYDEIEKSYSNVLTPNQRLQVTSAV
                     QVLAGLVGELEIRVVQPGASISVDSQVRGVSPLARVVRVNGGSHQLRVVKDGYESYQV
                     DAVVSSGERRILEVNLTPAQPESTHSPAPAAPAAPALPIPEPVTPIREQTPPPLTFGL
                     FLGPTVAPSFGGEADASCSKDVISSSGEARPGCSSSPLALGGLVGGRIAYRLSQGVAA
                     DFSGGYLGISRRLTRVIEATAADGSAYLGRAVRDERKLRGWFTMLGMSKTLGTRTPLL
                     LRLGAGVARMRLDAHVSGEFTSADTAQSGAFELDEDPRRIWIPFINPELRFGYRVAPK
                     WVLDAGISLWWGFPPSVSRTGDVWNFDTRRAFTQLDSGASEELTLRKESALGMMLIVV
                     PTIGAQFDL"
     gene            3543..6128
                     /locus_tag="VFQ61_15425"
     CDS             3543..6128
                     /locus_tag="VFQ61_15425"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET9955900.1"
                     /translation="MSRQTSWVITPLVFSAVLSCARERPEGAGASGIRAGSEPAALLG
                     STRQALAGTSAMVVARYGHAAVALPDGRVLVCGGRTASLSDGAARAATDHCEYYDPAR
                     STWSDAGAPTLPSPRAGLSLTLLNTGKVLVVGGFGTDSSVYGEAYTLGTSGFVGLEPL
                     LPRFAHSATLLKDGGVLLVGGSNADGEVVVQAQLRLPDAESLPLDARWENTGNRTLGA
                     HLHGAATLTNGDVMITGGMTESGSISDAVFVYSAASKQLSSRRSLPAPRYAHTATMLP
                     NGDVLVVGGVDQNGAFASKAYRFLQSTQAWQEAGTPMERTSHLAARLDRFVIVAGGVG
                     KKRDAENDVQYSVRLRTVEAYDPNTNTWTTLAPLQAGRDAATLTPVQTPSKMPALLIA
                     GGSFGSAIVPTSELIVPGLGGNGCQANSDCVTGHCSPDGVCCDSACDGACEACNEPGS
                     EGICKDVSGPPRGESRSCAGGFACAQGKCADRCEDKADCQTGYYCNSNHECVSPGVLG
                     SACNSADACTDGLSCSGGVCCEEACDGPCQQCNAKGQCILLEGAPNRNRDCADMPHDA
                     GCNPTCDGKHAECTFVAGAECGDASCNPTVHAARSIGTCNEQGRCVAEEQPCGAYGCI
                     DNVCRDECTKPEHCNSNLCQDGRCVDELGKGDDCNDTKDCMPGLTCVDGACCTEQACP
                     EGASCRTPGKAGDCMKLENSPCEANLECASEQCFDGVCVAACESKSNCGEGQECSNGA
                     CIPSSGAGGESGNPNAAPRCSADFKSSIVDGSTSSCEGYRCNPATGKCAATCKQSSEC
                     STGYLCDPEHHCRVPPERRPIEGCSVQPLQREAGSPASHVTAWLLGLGAVAFARRRRA
                     ARGSD"
     gene            complement(6526..8496)
                     /locus_tag="VFQ61_15430"
     CDS             complement(6526..8496)
                     /locus_tag="VFQ61_15430"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9955901.1"
                     /translation="MSGYNANQLAAGELSPGKMLGRYELLMSIANGGMGNVWAARLKG
                     TRGFRKLFAVKTILRTFDDPRMEQMLYQEATVASQVHHPNVVETIELGEDRGIMFLVM
                     ELIQGESLSFILREAFGQGGVPMHLAVNLIGQVCRGLQAAHELCDEEGNPVGLVHRDI
                     SPPNILVTYSGVVKIVDFGVATTSSSATCGSGEIKGKISYLAPEQLRGDAIDGRVDVF
                     TTGILLYLLTVGQHPFKAPAEAQTISRILSDEPAKRPSVIIDGYPAALEAVLMKSLAK
                     NRDERFQSAAEFLEALNDVMPEAFGPNCDREFADYIRDLLKSRAQERRAALRMAEELA
                     EKSSPEVSILSVPGLVAATPAPAAKQARRWPLFAGSAVLALGALITANPQFLQHFRGN
                     SARVAQVQATLPAGERAIGARAGADAVVGDRVAAPRLSVDPPNSASAQPSAPSSTRPV
                     GSNSNESAVAHRPAHGRGGRSERDVAGRDDTNSNKEEPAGSLPEASALASPVIEAAAH
                     DDTAGAAEPVGSAIAIVQPLASAEPSALPTVAQNAPPTTVASPTKAEGPRMLPAKIAH
                     QRLAINPNASAYRAELPPALERAGQSFSATVQICVSPAGRVTGVTILRSASPVVDSQI
                     LGALGRWRYNPMLESGHPVPFCYRLEYQVDPR"
     regulatory      10937..11254
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            11522..>13528
                     /locus_tag="VFQ61_15435"
     CDS             11522..>13528
                     /locus_tag="VFQ61_15435"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HET9955902.1"
                     /translation="MCVRKRDGSREPVDVNKIVRAVSRAASDLHSIDPLRVALRTIGG
                     LYDGASTQALDELSIQTAAGLIAEQPQYSKLAARLLSTYIEKEVVTANIHSFSQCIEQ
                     GQRLGLIHPRLGEFVGRASRKLNTAIDPSRDRLFEYFGLRTLYDRYLLRHPDTRRVLE
                     TPQYFWLRVAASLSDNVSEALAFYELISSLEYVPSSPTLFNSGSPHEQLSSCFLLDSP
                     ADHLEDIYQRYTDVALLSKFSGGIGIAYSRVRSRGSRIEATNGHSSGIVPWLKTLDAS
                     VAAVNQGGKRKGACAVYLETWHADLEEFLELRDGTGDPARRTHNLNLAHWIPDLFMKR
                     VEADGQWSLFDPKRVPELCDLWGHEFESAYVAAEKQGKAARCVGARDLYARMMRTLAE
                     TGNGWFTFKDRANQTSNQTAQPHNVVHLSNLCTEILEVTSPDETAVCNLGSINLARHT
                     LGPSEGQPARFDFEKLAHTVTLAVRHLDRVIDLNYYAIPSAARSNARWRPVGLGVMGL
                     QDAFFELGLPFDSEPARALSRRIAEEIYFHALNTSADLAAERGAHPAFAETRAAQGEL
                     QFDYWSQKPEAPERFEALRQRIRQHGLRNSLLIAVAPTATIAAIAGCSECIEPQVSNL
                     FKRETLSGEFLQINRYLVRDLEKLGLFSEEFHDRLKLAEGSIQGM"
BASE COUNT         2507 a         4344 c         4191 g         2486 t
ORIGIN      
        1 atcgtgcagg tgttcgactc gggaatcgac aacccgacag ggcgcccgta tttggtaatg
       61 gagctgctgc ggggcgaaga cctgcgcacg acgttggccc gaacgggcgc gctcgagccc
      121 atctgtgcgg gaaggctcgc actacaagcc acactcggcc tgacccgtgc ccacgcgctc
      181 ggcatctttc atcgcgacat caagcccgca aacctgtatc tcgctcgtcg agactccggc
      241 gaaatttgcg tgaaactgct cgatttcggc atcgccaagg tgcgcgcgga aagcggacta
      301 ttcacgaccg aaaccctcac ccgcacgggt ggaatcctcg gcaccccgtt gtatatgtcg
      361 ccggagcaaa tccgcggcgc atcggacgtt gacgcgcgca gtgatgtctg gtcactgggc
      421 gtcgtattgt tcgagtgctt gagtggtcaa ctcccgttcg atggcaacgg ctcgatcggc
      481 gagttgatgg cggcaatctt gaccaccaac gcgcgtccgc tgcgcgagat agcaccttgg
      541 gttccggatg ctctggtcga cgtggtcacc caagccttgg agcgagaccc cgcgcgacgc
      601 ttcgccgatg caagcgcgct cggcgaagcg ttggctcgtg ttctaccgag tggcgtcacg
      661 gtgctcgaaa gcgcgctcgt ttccgctcgc ccgctcgacc agacgcgcgt ctcgggagag
      721 gttccggtgg tggaagccca aatgggtctg gcggcgccgt cgacgccggt tgggctcagt
      781 cctcgcccga tcccggatgc gagctcggac gcctggcgac tagtgcgggc ccgcgccgag
      841 acgagcgact ccgatctcgc gagccccatc ttgcgacgct cggcaggcaa cgacgggagc
      901 ggtgcggccg tccccggatc caacagcccg agcacggcgg tgattgaacc gcacgccaat
      961 gcgcctctca ctcccgccca agaacattgc aatccgactt ggctctccgg caatcccccg
     1021 cccgtgcagt catcgctgcg agacccgggc ccagtctcgc ggacacgttg ggggctcccg
     1081 atcgcgtttg cgctcgggct gctcggcgcg gcgataacca tcgtgctgtg gactacgtcg
     1141 cgtcacacgt tggtgtccgt ttcatcgagc gaggggtcag cactgaactc agcggcccca
     1201 tactcgccca aaaccccacc gcctcaactg cccgcaaccg cgaccgcgag cgttcccacc
     1261 ggggcgcctc aagtcgagct cgcaaacgat cctcctcccg tagttgcggc ttacgtcgaa
     1321 attcaagtgc cgcttggggc gcgcgtcttg gcggactcac agccgctcgc ggtggaaact
     1381 cgcggcagcg caggctacgt gaagctgccc tccaagcccg atcgcggcgt tcgcctggag
     1441 cttactggag tgggcccgaa ggtgaccgaa ttggtgcaga ttcagcaagg caaagcggtg
     1501 ccgggcgtgc tcacgcgaag aaccgaagcg agcttgcagc gaacccccaa gcccagcgag
     1561 ccaccggctg ttccgccggc tcctcgcggg gtctttttcg acttggagga ggagcccgcg
     1621 ccggctccgc atcccgctca gcggcagccc aattcagctc cgcggggctc cctcaatccg
     1681 ccgggctcgg ccgccaaata ccccgatccg cctcctagct cgccctagct gctcggcccc
     1741 aacgcgcgga gatcgagcgc gcggcgctag gaacgcggtg cgaggcgggt tacagacgtg
     1801 gtctggctca acgcactcca ctcgccgcat tctactgatg ttgcgcgcaa cgctccgcag
     1861 ccctgccaac cgcatcgtgc catcccgatc cgcgctctcg cgcccaggcc tcaacgctct
     1921 cgtcgtgctc acggcgctgc tgaccgcgag gcatggaagc gcacaagcca gcgtcagcag
     1981 cgcgtctgcc aacggcacgc cagcggccgc gaacgcagaa gccccgaatc cgagtgaccc
     2041 gagcgcgcgg gagcccgatc cagcgcgtcg caacgccgcc atcgagcact tccgacgcgg
     2101 gctcgagctc gcccgcaagg gcaactgcag cgcggccctg cccgagttct tgatctcacg
     2161 cgaactgtcg ccgctcgtac ccgccctaat caacgcggca atctgcttgc aggatctcgg
     2221 gcgtccggca gaggccctcg acttgtacga cgagatcgag aagtcgtatt cgaacgtact
     2281 cacccccaat cagcgactgc aggtcacgtc tgcggtccag gtgttggcag ggctcgtggg
     2341 agagctagaa atccgtgtcg tgcagcccgg agcctcgatt tcggtagact cccaggtgcg
     2401 cggtgtttcg ccgctcgcgc gtgtcgtgcg ggtgaacgga ggctcgcacc agctgcgcgt
     2461 cgtcaaagac ggatatgagt cctatcaagt cgacgccgtc gtatcctccg gtgagcgacg
     2521 catcctcgaa gtgaacctca cgcccgccca gcccgagagc acccattcgc ccgcgccggc
     2581 ggcgcctgca gccccggcgt tgcctattcc agaacccgta actccgattc gagagcagac
     2641 gccgccgcca ctgacgtttg gcttgtttct cggccctacc gtagctccat cgttcggcgg
     2701 ggaagccgac gcgagctgca gcaaggacgt cattagctcg agcggcgaag cgagacccgg
     2761 ctgcagcagt agtccgctcg cgctcggcgg cttggtcggc ggtcgcatcg cctatcggtt
     2821 gtcgcaaggt gttgcggctg atttttcggg cggatacttg gggatctccc gacgtctcac
     2881 gcgggtgatc gaagccaccg ccgcggatgg cagcgcatat ctcggccgcg ccgtgcggga
     2941 cgaaaggaaa ctccgcggct ggttcacgat gctcgggatg tcgaaaaccc tcgggacgcg
     3001 cacgcctctt ctgctacgac tcggcgcggg cgtcgcgcgc atgcgcctcg atgctcacgt
     3061 gagtggtgag tttacgagcg ccgacacggc tcaatctgga gcattcgagc tcgacgaaga
     3121 tcccagacgc atctggatcc ccttcatcaa cccggagctc cgcttcggct acagagttgc
     3181 accgaaatgg gtgttggacg cgggaatttc cctgtggtgg ggttttccac cgtccgtctc
     3241 ccgcaccggt gacgtttgga acttcgacac ccgtcgcgct ttcacgcagc tcgactccgg
     3301 cgccagcgag gagctgacgt tgcgcaaaga atccgcgctc ggaatgatgc tcatcgtcgt
     3361 accgacgatc ggcgctcagt tcgacttgtg accgcgcagc gccgaaagcg ggagttgcga
     3421 cggcgcgact tgcgacggcg cactgacacc ctgctgctac caaatgacca tcgtgaagcg
     3481 ggcgcgcgga acaacgcctg tggcatactc gcgacttggg aaagcgcatg tcgagccacc
     3541 gcatgtcgag gcaaacgagc tgggtcatca ctcccttggt cttctccgct gtcctgagct
     3601 gcgcgcgtga acggccggag ggggcgggcg cgagcggcat ccgcgccggg agcgagccgg
     3661 cagccctgct gggctccact cggcaagcac tcgcgggcac gagcgccatg gtggtcgctc
     3721 gctacggcca tgcggcagtg gcgttaccgg atgggcgcgt gctggtgtgc ggcggccgta
     3781 ctgcgtcgtt gtccgacggg gcggctcgcg ccgcgaccga ccactgcgag tattatgacc
     3841 ccgcccggtc gacttggtcc gacgccggtg cgcccacgct gccctcgccc cgcgcgggcc
     3901 tctcgctcac gctcttgaat acgggcaaag tgctcgtggt cggcggattc ggcaccgatt
     3961 cctcggttta tggggaagcg tacacgctcg ggaccagcgg cttcgtcggg ctcgagccgc
     4021 tgcttccccg cttcgcacac agcgctaccc ttctgaaaga cgggggcgtg ctgttggtcg
     4081 gcggctcgaa tgccgacggg gaagtcgtgg tccaggctca gctgcgcttg ccggacgcgg
     4141 agagcttacc tctcgacgcg cgctgggaaa atacgggcaa ccgaacgctg ggagcgcacc
     4201 tgcatggcgc ggcaacgctc accaacgggg acgtgatgat caccggaggg atgaccgaga
     4261 gcggctcgat ttcggacgcc gtcttcgtct actccgccgc cagcaagcaa ctcagctcac
     4321 gccgcagttt gcccgcgcct cgctacgcgc acaccgctac gatgttgccg aatggcgacg
     4381 tgctggtggt cggcggggta gaccagaacg gcgccttcgc gagcaaggct tatcgcttcc
     4441 tgcagagtac gcaggcttgg caagaagctg gcactccgat ggagcgcacg agtcacctcg
     4501 cggcgcgact cgatcgattc gtgatcgtcg ccgggggcgt gggtaagaag cgggacgccg
     4561 agaacgacgt tcaatactcg gtgcgcctgc gcaccgtcga agcctacgac ccaaacacca
     4621 atacctggac cacactcgcc ccattgcaag ccgggcgaga cgccgcaacc ctcacgcccg
     4681 tccagactcc gagcaagatg cccgccttgc tcatcgccgg aggctcgttt ggaagcgcga
     4741 tagtgcccac atcggaactg atcgtgccgg gcctcggcgg aaacggctgt caggctaact
     4801 ccgattgcgt gacgggccat tgttctccgg atggcgtgtg ctgcgacagc gcttgtgacg
     4861 gtgcgtgtga agcctgcaac gaacccggca gcgaaggaat ctgcaaagac gtgagcgggc
     4921 cgccacgcgg cgagtctcga tcgtgtgcag gtggctttgc ttgcgcacaa gggaagtgcg
     4981 ccgatcgctg tgaggacaaa gcggattgcc agaccggcta ctattgcaac agcaaccatg
     5041 agtgtgtctc gcccggagtg ctcggttcag cctgcaattc cgcggatgcg tgcacggacg
     5101 ggctgagttg ttcgggcggc gtctgctgcg aggaggcctg tgacgggccg tgccagcagt
     5161 gcaacgcgaa gggtcaatgc atcctgctcg agggtgcgcc gaaccgcaac cgagactgcg
     5221 ccgacatgcc gcacgacgcc ggttgcaatc caacctgcga cggtaaacat gccgagtgca
     5281 ccttcgtagc cggggcggag tgtggtgacg ccagctgcaa tccaacggta cacgccgcgc
     5341 ggtcgattgg cacttgcaac gagcagggcc gttgcgtggc cgaggagcag ccatgtggcg
     5401 catacggttg cattgacaac gtgtgccggg acgagtgcac gaagcccgag cactgcaact
     5461 caaacctatg ccaagacggt cgttgcgtcg atgagctagg caaaggtgac gactgcaacg
     5521 acaccaaaga ctgcatgcca ggcttgacct gcgtggatgg tgcgtgttgc accgagcagg
     5581 cctgcccaga gggtgcttct tgcagaacac ctggcaaagc gggcgactgc atgaagctcg
     5641 aaaactcacc ctgtgaggcc aacctcgaat gtgcctccga gcaatgcttc gacggtgtgt
     5701 gcgtggctgc gtgtgagtcg aagtccaatt gcggggaagg ccaggagtgc agcaacggag
     5761 catgcatccc gagttcaggt gctggcggcg agagcggaaa ccccaacgcg gcgccccgtt
     5821 gttcggcgga cttcaagagc tccatcgtgg acgggtccac gtcatcgtgc gagggatacc
     5881 gctgtaaccc ggccacgggc aagtgcgccg cgacctgcaa acaatcgagc gaatgcagca
     5941 cgggatatct ctgtgatccc gagcaccatt gtcgcgttcc tcctgagcgg cgtcccatag
     6001 aaggatgctc cgtgcagccg cttcagcgcg aagccgggtc cccggcaagc cacgtgacgg
     6061 cctggcttct cggccttggt gccgtcgcgt tcgcgcgacg acgtcgagcg gcacgcggca
     6121 gtgattgagc tctcgagtca aatcgcactt tcgcgcaacc gggctcagcg ctcgcgcccg
     6181 ctcagggccc gttcaccgac ggccccgacg agtccccgag gagtgctcga gaggctattc
     6241 atctgttagc ggccactcaa tcacgagcgc gaagcgcgtc cgattactcc gccgcgcagc
     6301 ctgtattcgt ccctgcctca cccagcaccg cgccggcccc catagcgcat ccccaagctg
     6361 atgctccatc ttgagtcctg gataagcccc gcacgatcgg cgcgtacacg ccgccggaac
     6421 atgcgcgcga atgaatgctg ccgcacggcg acccggcgag ggttagacgc gcgaccaagc
     6481 gcctgcgcgg cacctactca cgcgcgcccg gacgagtaat cacggtcagc gcgggtcgac
     6541 ctggtattcc aaccgataac agaacggtac cgggtgcccg ctctccaaca tcggattgta
     6601 gcgccagcgc ccgagcgctc cgaggatctg gctatctacg accggggagg ccgagcgcag
     6661 aattgtgact ccggtcacgc gccccgccgg cgaaacgcag atctgaaccg tcgccgagaa
     6721 ggattggccc gctcgttcga gtgcaggagg taactccgcg cggtaggcag aggcgtttgg
     6781 attgatggcc aagcgctgat gagcaatctt ggccgggagc atgcgagggc cctccgcctt
     6841 cgtgggtgac gcgacggtgg tgggaggcgc gttttgagcc acggtaggca acgcagaagg
     6901 ctcagcgctc gccaacggct gaacgattgc aatggcggag ccgaccggct ctgcagcgcc
     6961 ggccgtatca tcgtgagccg cggcttcgat cacgggagag gccagggcgc tggcttcagg
     7021 cagtgaacca gccggctcct ccttattact gttggtatcg tcgcgccctg cgacatcgcg
     7081 ctcggagcga ccgcctcgtc cgtgagcggg gcggtgggcc accgcgctct cgttcgagtt
     7141 cgagcccacc gggcgcgtcg agctcggcgc cgaaggttgc gctgacgcgc tattcggagg
     7201 atcgaccgac aaacgcggag ccgcgactcg atcaccgacc accgcgtctg cacctgctcg
     7261 cgcccctatc gctctctcac ccgccggcaa ggtagcctgg acctgagcaa cccgggcgga
     7321 gttgccgcga aagtgctgga ggaactgtgg atttgcggtg atgagggcac cgagcgcgag
     7381 caccgcactc cctgcaaaca acggccatcg gcgtgcctgt ttcgccgccg gagctggtgt
     7441 tgccgcgact agcccgggca ccgagagaat cgatacttcc ggagaagact tttccgccag
     7501 ctcttcagcc atgcgcagag cggctcttct ctcctgggcg cgcgacttca atagatcgcg
     7561 aatgtagtcg gcaaactccc gatcacagtt cgggccgaag gcctccggca tgacgtcatt
     7621 cagcgcctcg aggaactcgg ctgcagattg gaagcgctcg tctcgattct tggcgaggga
     7681 cttcatcaac actgcttcga gcgcggctgg atacccgtcg ataatgacgg acggccgctt
     7741 ggcaggctca tcactcagga ttcggctaat cgtctgtgcc tccgcgggag ccttgaaggg
     7801 gtgctgcccc accgtcagca ggtacagcaa aatacccgta gtaaagacat ccacgcgtcc
     7861 atcgatggca tctccacgga gctgctccgg agcgagatac gagattttgc ccttaatctc
     7921 gccggaacca caggttgcgc tcgagctcgt cgtagcgact ccgaagtcca cgattttcac
     7981 cacacccgag taagtaacca ggatgttggg aggcgagata tcgcggtgca ctagaccaac
     8041 ggggttacct tcttcgtcgc acagttcatg agcggcctgt aacccccgac acacctgtcc
     8101 gatcaagttg acggcgaggt gcattggcac gcccccctga ccgaaagcct cacggaggat
     8161 gaagctcagg gattcgccct ggatgagctc catgaccagg aacatgatgc cgcgatcttc
     8221 acccagctcg atcgtctcca ccacgttcgg gtggtgcacc tgcgaggcaa ccgtggcctc
     8281 ctggtagagc atttgctcca tgcgcggatc gtcgaacgtg cgcaggatgg tcttgaccgc
     8341 gaagagctta cggaagccgc gcgtgccctt taggcgcgct gcccagacat tgcccatgcc
     8401 gccgttggcg atcgacatga gcagctcata ccggccgagc atcttgcctg gtgaaagctc
     8461 acctgcagcc aattgattcg cgttgtatcc cgacacgacg atcctccgtg cctttgcctt
     8521 ctctaagcta actcaagcgt tttgcgacgg tcttcgacac ctgactcgtg ccctgaaggt
     8581 cgctcgtggt cggtcgccga ccgcaccaca tgacggcagc acccggtcct cgcgatgtga
     8641 gttttcgcga acctggtcac caacgcagtc acgtgaaggt ttggccggca cacctgaagc
     8701 attacggact aagagtagac ttgtttcgga caaactgccc gcactcaaag accgtaacga
     8761 tgatttttct gttccgtgaa gttgagcggc ttcacgcgca gcacccagtc gagttgtggg
     8821 gatgctcgat tccgcgaagg tcgattcgcg ctcgatccga gcgtcgtggc tcgatgcgag
     8881 ctggcatgcg ggtgcggctt gcacacgcga gttcgcgaac cctcgctccg aagtgcaagc
     8941 tggctggttc gacaacatgg attgcgcagc gcgctgtgcc acgacgagaa cttggcgcag
     9001 cctcgtcctg cgccagaccg cttttggaag aacttcagca agcactcccg tcatcgcgat
     9061 ctcacccacg gccgcaagct ggagtgcata gcgcacggga gttacccgga gctctcggag
     9121 cctcggccga gagcaggatt cagctccgat acaagagttc agagccgcga cagcgcgcgc
     9181 ggctctgtac ccctcattga gctgaattgc gcttgttggt gccattgctt ctctttctta
     9241 cgctgccgct tctggcttcg acgctgacgt ttgctccgag cttcggctac ccggaggagc
     9301 ttggaggcgt tcccaaccta cggggaaggg ctctggtttc tgccatccag taatccgctg
     9361 ttgctttcgc gctcgggtgt ctcgtatccc ggtgtctcgt ctctctcggc tcgcgtgcgg
     9421 gggttccaat cggtcggtct tgcgctctcg cgatgcatcg gggtccggcg cctccgcttc
     9481 tcgggtccgc cgtgacagtt gtccgctgcg ctgggcgttc ctgggctggg cattgctgtg
     9541 ccggtattcc tgcgctgggt acccctgcgc cgagtatccc ctgcgccggt ttgtacctac
     9601 gcgttggagg ctcctcataa ttcgaacgct cgctccgagg ctccccagag cgtcggctaa
     9661 aggccgggcc gtgcgaagcg aacgctctga tctccgccgg cgatgtttgg ttcgcccatc
     9721 gagccgactg ggagcgcttg aacgcgacca gccgccgatg aacgagcatc cctctcgtgc
     9781 atccgtcgcc cggatgccgc tcctctcaac aggcacattt acacgaaacc gaattcgtag
     9841 ggagaggctc ttcgcgattg ggaatcaaat ttgtaagtgc gttgggcgtg aaagctgaca
     9901 tgcaccttgc tctctaaaag agcagcgcga ttgccgagca ggccgtgctt cgcttctttt
     9961 caatatgtct taccgaagca cgaatgggct gaaaatcagc aagtttgctc gcatgcatgt
    10021 cagcccattc gagcgatgat ccggagccag ggataaccca ctatccgcgt tcgcatctct
    10081 cgcctacacc aaactcccgt cctgacgccg ggacctgtcc agcgtcgctc ggccgcgcaa
    10141 agcgctggcc ccggctccca tagcatccaa ttacattcac tcgggccccg ccaccttcga
    10201 agcaaacgcc tcggaggaat ggcgctcacg gcatgtccga agaactgccg gtagaaaatt
    10261 gcgaaagtag caaccacgag aaagcgtgaa tttccgccgg caatcccgac acgccgattg
    10321 ggacgcctcc gccgcggccg ggaaaaacgc gcaaacgcag cgaatctgcg aaacacaatc
    10381 ggagactact ggcgatcttc gaacccagtg gtctggccaa agccgcgaca gagcttccgg
    10441 gcgctgacaa aaagaccacg attgtgagcg cgccgtcgtg tttttaagct cgagcgtcca
    10501 tacaggtgtg cacgcgctct gttctctcac gcagcgccgc gatgtgtcac atagccgaag
    10561 ccacgccgtc gaaaggcgcc ttgccgggcc tagcgaagct ctgccccttt aattcttcga
    10621 cgcgcaccac cctctctggt ggagcgggcg ttaccggcag cttcttccta tgtggagtcg
    10681 agaccacaaa actccgatgc tcgtattcag acgaagtcgc gagggggtgg cctgttgggc
    10741 tcggggcgca gctcgttcat caccgatggc aattccccag cggaggcggt gcgtgcagac
    10801 tttctcgcag agcccagaca acgatccgac gatgcaaaac caacctagag cggccgcctg
    10861 aacggaccag caggtcagtt tgagctgatc cgatgcgcag cccaatatct cgttgacact
    10921 gatcgtcgca gcccaatata taggtgctgc ttccggtgcc tgagcacgtg cccaggagaa
    10981 acacgcgccc aggtgaatag ggaacccggt gagaatccgg ggctgccccg cagcggtatg
    11041 tgagaacgaa cgtcgcccga aacctcagtt ctgcactgag ctcgcaagca ctgagtcgga
    11101 ttcctcgaga ttgagagatc ggggtctcgg gctcgggaag cggtgactac taggttggcc
    11161 tttgcctctt tcggcagcgc tttgtcgtgc ccagtcgaag gagagaaggc ctttcttacg
    11221 agcccgaaga cctgccgaaa gccctgcgcc ggacaattgg cgtgggtgac agccctcctt
    11281 cgcgggaagg gaggcggtgt tcggcggagc gcgtctgcgt tccctcgctc gctgacttcc
    11341 attcagcatc ggaatcgaga gttcgaggga gctctcttgg gcgattcggg cgtgcgcgtg
    11401 tgtggccgaa aagctcgatg cgcacgagaa atggcgagaa attatggtcc aggcattcga
    11461 cacacgtcgt gaggccgcgc ctcgcgcaac gttcagtccg agttccgatg tggcacaaag
    11521 catgtgcgta cggaagcgcg atggcagccg tgagccggtg gacgtcaaca aaatcgttcg
    11581 tgcggtgtcg cgcgcggcca gtgacctgca cagcatcgat ccgctgcgcg tggccctgcg
    11641 gacgatcggt gggctgtacg acggagccag cacgcaggcc ttggacgagc tctcgattca
    11701 gaccgccgct ggtctgattg ccgagcaacc ccagtactcg aaattggcgg cccgtttgct
    11761 ctcgacctac atcgagaaag aagtcgtgac cgcgaatatc cattcttttt cccagtgcat
    11821 cgagcagggt caacggctcg gactgattca tccccggctc ggtgagttcg tcgggcgggc
    11881 ttctcgaaag ctgaacacgg ccatcgaccc cagccgcgat cgactattcg agtatttcgg
    11941 gctgcgtacg ctctacgatc gttatctgct ccgtcacccc gatacgcgcc gcgtgctcga
    12001 gacgccacag tacttctggc tgagagtcgc ggcgtctctg tcggacaatg tatctgaagc
    12061 cttggccttc tatgagctga tatcgagcct ggagtacgtg ccgagttccc ccacgttgtt
    12121 caattcgggc agcccgcacg agcagctttc ctcgtgtttc ctgctggact caccggcgga
    12181 ccacctcgaa gacatctacc agcgctacac ggatgtcgcc ctgctctcta aattttcggg
    12241 gggaatcggg attgcgtata gtcgggttcg cagccgcggt tcgcgaatcg aggccactaa
    12301 cgggcactcg agcgggatcg tgccttggct caaaacgctg gatgcttcgg tcgccgcggt
    12361 caatcaaggc ggtaagcgca agggcgcatg cgcggtttac ctggagactt ggcacgcaga
    12421 tctcgaggaa tttctcgaat tacgtgacgg cacgggtgat ccggcccgtc gcacacacaa
    12481 tttgaatctc gcgcactgga ttcccgatct gttcatgaag cgagtggaag ccgacgggca
    12541 gtggtcgttg ttcgatccga agcgagtgcc tgagctctgc gatttatggg gccatgagtt
    12601 cgagtccgct tacgttgcgg cagaaaaaca gggcaaagct gcccgatgcg tgggcgcgcg
    12661 tgatctctac gcgcgaatga tgaggaccct ggcggaaacc ggcaatggct ggttcacatt
    12721 caaagatcgc gcaaaccaga cctcgaatca gacggctcag ccgcacaacg tggtgcacct
    12781 ctccaacctc tgcaccgaga ttctcgaggt cacctcgccg gatgaaaccg cggtttgtaa
    12841 tctcggttcc atcaacctcg cgcgacacac gctcgggcct tctgaaggtc agccggcgcg
    12901 attcgacttc gagaaactgg cccacacggt cacacttgcc gtccgccatc tcgatcgagt
    12961 gatcgatctc aactactacg cgattccgag cgccgcccgc tccaacgcac gctggcgccc
    13021 ggtggggttg ggcgtgatgg ggctacaaga tgcctttttc gaactcggcc tccctttcga
    13081 cagcgagccc gcacgcgccc tgtcccggcg catcgcggaa gaaatctact ttcacgcact
    13141 caacacctcg gccgatctgg cggccgagag aggggcacat cccgcatttg cggagacgcg
    13201 cgcggcgcag ggcgagctgc aattcgacta ttggagtcaa aaaccggagg cgcccgagcg
    13261 cttcgaagcg ctgcgccagc gcatccgaca gcacggcctg cgcaattcac tgctgattgc
    13321 cgtggcgccc acggcaacca tcgcggctat cgccggctgc tcggagtgca tcgagccgca
    13381 agtctcgaac ctgttcaaac gcgagacatt gtccggggag ttcctacaga tcaatcgata
    13441 tttggtgaga gatctggaaa aactcggcct attcagcgag gaattccacg accgactcaa
    13501 gctcgccgag ggttcgattc agggaatg
//
LOCUS       DASTKU010000450        13952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1870458, whole genome shotgun sequence.
ACCESSION   DASTKU010000450 DASTKU010000000
VERSION     DASTKU010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13952
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1870458"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1345)
                     /locus_tag="VFQ61_15440"
     CDS             complement(179..1345)
                     /locus_tag="VFQ61_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955903.1"
                     /translation="MRTFRRVAATSTVSVLFTSALATSVVFSSCLYEIENECGLRLQC
                     PEFVVAGATNQGGEGGESPSAGGSSSGGNSDIADSGGAGGEAGGGGAPVVIPCNGACG
                     GATPVCDEELGKCVECVSAEDCSDGVCEPTSRTCVECVDSSQCGEDRTYCDAKKNVCV
                     HCLEDKDCSDPSASRCVANECTACATNADCRHLDGKGVCDAKECVQCSVTDESPCDGN
                     SCNPARRECTETPVGTRADCEPCVADSECVGGDSTDPDRRCVPMEFKGVKREGGFCLR
                     RVAKECQRPYLIGLAAESLSGAPSENYCGIDHENVRCEAILDLSPSARACTAGDDATC
                     GCERDANGNCLAPGEGGLCRDFVTVKNRCTYRCGNNDDCPDGKKCGGDPVKYCQ"
     gene            complement(1357..2418)
                     /locus_tag="VFQ61_15445"
     CDS             complement(1357..2418)
                     /locus_tag="VFQ61_15445"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9955904.1"
                     /translation="MARIGVAQTPGSTLLTTTLLPATPSSASPTSGAPNQGETKDKEA
                     ERLFYAGVAASDRGDAEAARLAFSKSYSLSGEPHALRNWGVSAIESGHWVEGAKQVQR
                     YLAARPPGITEEETASLQEMLSSAERKIGRFVFVTDIEGAEVAVDGEPAGVTRSLPVC
                     YVEPGVHTISARKAGYAPFETRVEAKAGNATVSYIKMQVQSEPPSSVAPTPSPEPSPA
                     LDAPRSHFLNRTTVTLGLATLTLGAAATWYGAYAARNSAEDQAKTLRAMAERESSSGQ
                     CTGQASNLLACRKLQDQVDRVEHWRSVAAVAEFATFGLAAASLGTWLLWPQAKPPQTL
                     RVMPWTGPHLTGLRAQGSF"
     gene            complement(2488..3312)
                     /locus_tag="VFQ61_15450"
     CDS             complement(2488..3312)
                     /locus_tag="VFQ61_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955905.1"
                     /translation="MTTRDDSKLPASAAGNRALRLVYEAPREMAAKPELAAERPKRPS
                     ALELTEIQFTPEQRKEYLRLYREGLEHEARLDAARLAEEARLQEQARIQEEASKCQEE
                     ARLAELAELAALAAQRAALPPLASSPTVGGTSGSNIKLFAVAVAGLLVGALVVARPWL
                     SSQPSIPPAAVVSTAVPNLDVRAQPATSPLAASARSPEMAPVASVPAPPPVKNDPRPR
                     AHLNSTQARVPIPRGVASSASPTTLNPQPEPTAQSKKRIPLARGFRMSGESGAKEP"
     gene            complement(3315..5066)
                     /locus_tag="VFQ61_15455"
     CDS             complement(3315..5066)
                     /locus_tag="VFQ61_15455"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9955906.1"
                     /translation="MNHRKVLAGSEADQLQHLPDPLFKPGDLCGAYEVLGLLARGGSA
                     DVYRARHLFLDVTRALKIMRRGHRDLRGVRRRFVREARNVVTIEHPNVVKVVDGGEHD
                     GRAWIAMELIPDGLLRSRIQPDKPMPIVAALRHMLAICDGVGAAHQRRLIHRDLKPEN
                     IAMKGETPIILDFGIAKRLGLEVALNTSQALLGTPAYASPEYLLSREDSVPVDERTDV
                     YSLGHIAYELFLGRHAYANADGSFPSVVQLVRRQVEPVHGHVTPLTDLIPGFPTYAWA
                     EVGRALSKAVAKRHTNAGEFGAALSLVLRRYLEERGQTPEFYEQLSLPASRLEDLDSS
                     HSRGAPGNGESCAIEPSVTDSSPAAEEPQIHYNGVTQPVEFTLPTPNQVSSARHHRLA
                     RSRREQRTSTPVAPSTTSTLPPTRPVLSYRRLGYLTGAGFALSLAVLGSLHVLAAALP
                     HQPLVPREPSAPSLASAPPVRTLSEPPSSSLSVIYNPTLQLTRRDSTPSAIGLRAAAP
                     VRPAKSAPIQPPGPKTTLAPPERASRIKSATAPSAKTGDSGNDWRVSPLGIINPYPDD
                     PPPAVGRSPTASTTPRR"
     gene            complement(5122..6174)
                     /locus_tag="VFQ61_15460"
     CDS             complement(5122..6174)
                     /locus_tag="VFQ61_15460"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9955907.1"
                     /translation="MRSGEPPFNLVIAGKYRLIGLLGEGHTSIVYEAVHEALGSALAI
                     KVLRSELRERADIVARFRVEGRALAQLDHPNVLRVHDVGMLDDGRPFMVLQRLWGHTL
                     AEEIKRSGALAPERAVTIARGILAALGRVHSLGMVHRDLTPQNVFLNEDGSSEALPVL
                     LDFGQAKVLSSQPLPLNAPTPACSTPEDVCWGTPRTASPEYVELGEIDARSDVYSAGV
                     VLYWMLAGRDPFHDALTTEEVFRAQVLRQADPPSRWTLHQVPAALDEVVMQALEKNPK
                     QRFDSVEQFAAALDEAMRVQPPAAGASANAGRPWPTTQFGAAAFVVGIAGFALVTLLL
                     LFPSAVPFSEAWLKSR"
     gene            complement(6177..6833)
                     /locus_tag="VFQ61_15465"
     CDS             complement(6177..6833)
                     /locus_tag="VFQ61_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955908.1"
                     /translation="MREKVLPEAERVAIAAEIRELLALRDQFGRRYWTQASLGEACGG
                     LSQQVIYLAQQPNRVGPAVRDALLQVLKVPMSQLLEKHRPLIDAPEEKSTARKKRGEP
                     SGKEKGKEQIGNPRTAPRGGPAPLPARVLLDPLEAYRKVMEALQEDGYEQNDIRLVLA
                     QVAFEFTESPSIPGTAEDILRVYRAARGMLEPQRRARGSSKQGSASGVRVTKSRRRVG
                     "
     gene            7463..8905
                     /locus_tag="VFQ61_15470"
     CDS             7463..8905
                     /locus_tag="VFQ61_15470"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9955909.1"
                     /translation="MARGLAVEATWIGRTVGSGWIVDSVIGVGGSATVFRATNAEGQS
                     AAIKLMHHQLALSWTKRFEREAKLLQALSHPAIPELYEFGRFGGVPFLVLELLMGQSL
                     DVLCKDARQLDVARIIAYAADALAALEVVHDRGIVHRDLKPSNLLVTDDGVLKVLDFG
                     AAVHVETSLLGPDSLTSGLLGTPAYMPPEQARGRWDMVDHRSDIWSLGAVIFTLLSGE
                     HVHPAQTSNEQLSFAMTRTARSLGRLVPGLSAGLVRVVDRALAYERQERFQSAAEFRA
                     ALLGSLRDNSATTRDDACVTVREFRAAPSHSAIDVHRHGPSRVLRRAGWVSGLGACVL
                     AAALIRLELSPNRNSIPSTIPVSPISSGAERPPSAPGASQVPILSSAHADSRPASVIS
                     SNVAVRRVAPYASERARLPLASARSAAPSPRTIPPEPAPAPVLARSAPSALAPDSALD
                     RRLGTHLGDSNGASPAGAEGSSASPLDLRR"
     gene            8937..10001
                     /locus_tag="VFQ61_15475"
     CDS             8937..10001
                     /locus_tag="VFQ61_15475"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9955910.1"
                     /translation="MFQLWRVPARGFRIIAGLALVSSFPRPALAESRSPVPDRAEMPS
                     SEAAPPSHARFQLESPEASASQTTGEASSNSRADGLFRAGREAFRRGYYAEACAKFAE
                     SDLLEPSPGAKLNRGLCEEQLGHFALSWRLLQESASALPPEDERVSIALQHVQALRGK
                     LAFLTVTLASSVPSTAQITADGAELGRAELGLPLAYDPGTHVLELSAPGFRSRRTELS
                     LGPGANRRVNLTLGAAVHEPSGVPLRASTTQHPWRTTSFALGGAAVTLLIVGATSGVL
                     ALNAKHEMQAACDTQLACSDAGLQAAARGGSYAAASTGAFIGALVTGGLGAALWFVTS
                     ESAHAGRKSGFDRRHMRWEF"
     gene            10004..11881
                     /locus_tag="VFQ61_15480"
     CDS             10004..11881
                     /locus_tag="VFQ61_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9955911.1"
                     /translation="MKTLSFRVRQRLQSSRVSSLALCLVVGGVACSNLIGADFDQAHR
                     APKNPSGGSSAQNSDATGARMFGSGGASSGAGGRSPRGGAAAGGTSEKSRPTAGGQAG
                     ASSGSDSSGGTVTVPEGGSGGDPTTCPNALEWKEVSETSIPTMGEEAHARSLMPGVGV
                     ASGKSYSGCIGAQISSDLLLFLDCNVIEGDTVTLNEGDASELRSVPYKITYMETTVSG
                     RFRMNAIRPGVPHQVPWSARAPMRGERVTVIGLTEDHKFAKRFAVTTLEQSSLEAAAP
                     CRGDCSSAQDDAAVLVFGSDDHLIAYCQTDPCEGLRHCVTTAELRAQWTTFSMLAAMQ
                     PLLWGDVSGDGRADAIAVQRFNLEVRTSLGSDWAEPTKWYDWERDQDYPGKEQVALGD
                     ITGDGMDDFVAVGPGLQYLPSLGDRFGSPVILDNKLTRAEAVRLGDVDGDGYDDVVLL
                     QADTLTLMTQVATTQPVVRQYDARVESGWLSFDLADVDGDSHSDALLAYTDRIEVLLG
                     SSKGLAANHTWLNDLQLQPPGVLFADVTGDGRPDAVRIDSGGMSAFPSVGDRFDPLAR
                     SLKGLRMGQRGNAFADLDGEGHTDAVVQDTSNMWIFLSSSKNFGSADDWLNIPWLGSR
                     F"
     gene            11925..13199
                     /locus_tag="VFQ61_15485"
     CDS             11925..13199
                     /locus_tag="VFQ61_15485"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9955912.1"
                     /translation="MTDTTTLRELLGEKPSSGDDSHQLEMHVVHSPDASALGRRFSLV
                     PGESALVGRRQGLELSLEDERTSRSHVRIVWDQRTRGYRFADLGSANGTFLNGQRLET
                     GVLSSNDVLRVGDTLLVCSLPLALTRFDEEVRRAAASCLPVLLLGETGTGKEVLAKRL
                     HEQSGRSGAFIAVNCAALPRELATAELFGHTRGAFSGAGNARDGLIRAAAGGTLFLDE
                     IAELPIDVQPTLLRCLQERKIRTIGADREVDVDLRLVAATNADLESLVGQGKFREDLL
                     ARLAHILLKLAPLRTRRWEILPLARQFAPELAFTANAIESLLLFDWPRNIRQLRSLIE
                     SLAVLKPSGARIHDADLAERIPGFANRDQARQAVSITSDNSTGERRTQLTALLQKHSG
                     NIASVARELGKPRSHVYRWMRTFRLSRRQFDR"
     gene            complement(13176..>13952)
                     /locus_tag="VFQ61_15490"
     CDS             complement(13176..>13952)
                     /locus_tag="VFQ61_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET9955913.1"
                     /translation="EAALAVLREAIASGVNHIDTSDIYGPHITNQILREALHPYPDDL
                     VLVTKVGAKRGPQGSWEPAFSAEDIRSAVHDNLRNLGLDVLEVVNLRIMIDVMGPAEG
                     SIEAPLSALLKLQQEGKVRHIGLSNVTAKQVEEARKLAEIVCVQNHYNLAHRSDDALI
                     DSLAAAGIAYVPFFPLGGFSPLQSTALSRVAKELGATPMQVALAWLLRRSSNILLIPG
                     TSSLDHLRENLQSVELVLPDAAKQALDRFAMGPSAVELSP"
BASE COUNT         2479 a         4338 c         4455 g         2680 t
ORIGIN      
        1 tcccgcaggg ctccgaaacg gttcaccggg ctgatcccgg ctgcgactcg ggccaccttt
       61 ccgactccag cgggggaagt caccctcctc ctctccctgc aacggccctc gcttagcctc
      121 cagcggctgg accccgagag ccccgtgatc tacgtcatga cttgcggaat cggaccgttc
      181 attgacagta cttcaccggg tcaccgccac acttcttgcc gtcgggacaa tcgtcgttgt
      241 tcccacagcg ataggtgcag cggttcttga ccgtcacgaa gtctcgacaa aggccgcctt
      301 ctccgggggc taggcagttt ccgttagcgt cgcgctcgca gccgcaggtc gcgtcatctc
      361 cggctgtgca agcacgggca gacgggctca gatcgaggat ggcctcgcaa cgcacgttct
      421 cgtgatcgat accgcagtag ttctcggacg gagctcccga gaggctctct gccgcaaggc
      481 cgatgaggta cggccgttga cactccttgg cgacgcggcg caaacagaat ccgccctctc
      541 gtttgactcc tttgaactcc atcggaacgc aacgtcggtc gggatcggtc gagtcgccgc
      601 cgacgcattc actatccgcc acgcagggtt cacagtcagc acgggttcca accggagttt
      661 cggtgcactc gcggcgggcg ggattgcagg agttgccgtc acaggggctc tcgtcggtga
      721 cggagcattg gacgcactct tttgcgtcac acacgccctt gccgtcgagg tgtctgcaat
      781 cagcgttggt cgcgcacgcg gtacactcgt tggctacaca gcgggacgcg ctgggatcgg
      841 agcaatcttt gtcctcgagg caatgaacgc agacgttctt tttggcgtca cagtaggtcc
      901 ggtcttcgcc gcactggctg gagtccacac attcgacgca cgttcgagaa gtgggctcgc
      961 agacaccgtc agagcagtcc tcggcgctga cgcactcgac acatttgccg agctcctcgt
     1021 cacaaaccgg tgtggccccg ccgcaggctc cattgcaggg aatgacgacc ggggcgccac
     1081 caccacccgc ttcgccgccg gcaccgccgc tatccgcaat atccgagttt ccacctgagc
     1141 tgctgccgcc cgcggaggga gattctccac cttcgccgcc ctggttggtg gcgcccgcaa
     1201 ccacgaattc gggacactgc agtctcaatc cgcattcgtt ttcgatctcg tacaggcacg
     1261 aagagaaaac cacggacgtg gcgagagcgg atgtaaagag gacggagaca gtggacgtcg
     1321 cggcaacgcg ccggaaggtt ctcatgggca cgacctctaa aagcttcctt gggcacgcag
     1381 gccggtcagg tggggtccgg tccagggcat tacgcgcaac gtttgcggtg gttttgcttg
     1441 gggccagaga agccatgtcc cgaggctggc tgcagcaagt ccgaaggtgg cgaactccgc
     1501 gaccgcggcg acactccgcc agtgctccac gcggtcaacc tgatcttgca gctttcggca
     1561 ggcaaggagg ttcgatgcct gacccgtaca ctggcccgag gacgattctc gttcggccat
     1621 tgcgcgaagg gttttggctt ggtcttcggc cgagttgcgg gctgcgtacg cgccgtacca
     1681 ggtcgctgcc gcaccgagcg tgagcgtggc caaccccaga gtcaccgtcg ttcggttgag
     1741 gaaatgcgaa cggggggcgt cgagcgctgg cgatggttcg ggagatggcg tgggcgcgac
     1801 cgaactcggc ggctctgact gcacctgcat tttgatgtac gaaaccgtcg cgttcccggc
     1861 tttggcctcc actcgagtct cgaacggtgc atacccggcc tttcgcgcgc taatggtgtg
     1921 tacgcccggc tcgacgtagc acacgggcag gctgcgcgtc actccagcgg gctcgccatc
     1981 cacggccacc tcggcacctt cgatatcggt tacgaacacg aaccgaccaa tcttacgttc
     2041 cgcactcgac agcatctctt gcagagaggc tgtctcctct tctgtgattc cgggcggccg
     2101 cgcggcgaga tatcgttgca cctgtttggc accctccacc cagtgcccgg attcgatggc
     2161 actcactccc cagttgcgga gagcgtgcgg ttcccccgac agcgaatacg atttcgagaa
     2221 cgcgagtcgg gctgcttccg cgtcgcctcg atcggacgcc gccacgccgg catagaatag
     2281 ccgttcggcc tctttgtcct tcgtctcgcc ttgattcggg gctccgctgg ttggggatgc
     2341 cgaagacggg gttgcaggca acagggttgt agtcaataga gtggagcccg gcgtctgggc
     2401 aacgccgatc cgtgccatcg ttaggcctag ggccagcgca aatccaagtc ttgcaaattt
     2461 tgccgaatgc tgacgcaacc caaccactca gggctccttc gcgcccgatt ctcccgacat
     2521 tcggaagccc ctggcaagcg gaatgcgctt cttggattgc gcggtcggct ccggctgagg
     2581 attcaacgtc gttggggagg cactagaggc cacgcctcgg ggtattggaa cacgcgcctg
     2641 cgtcgagttg aggtgcgctc gcggccgagg atcgtttttg acgggtggcg gcgcgggcac
     2701 gcttgcgacg ggtgccattt cgggcgaacg tgcgctcgcg gccagcggtg acgtggcagg
     2761 ctgtgcacgg acgtcgaggt tcgggacggc cgtagaaact actgccgctg gcgggatact
     2821 cggttgactc gacaaccacg gccgcgcaac gactaaggcc cctacgagca gcccagccac
     2881 cgccacggcg aacagcttga tattcgaccc ggatgttccg ccaacggtcg ggctcgacgc
     2941 caggggcggt agtgccgcgc gctgggcagc taacgcggcc agttcagcga gctcggcgag
     3001 cctggcttct tcctgacact tgctggcctc ttcttggatc cgggcctgct cctggagcct
     3061 ggcttcctct gcgagccgag cggcgtccag gcgcgcttcg tgctccaggc cttcacggta
     3121 gaggcgtagg tactctttcc gctgctccgg cgtgaactgg atttcggtga gctccagcgc
     3181 ggaggggcgc ttgggacgct cagcggctag ctccggtttg gcggccatct cacgaggcgc
     3241 ttcgtacacc agacgcaggg cgcgattacc agcggccgag gccgggagtt tggaatcatc
     3301 tcgcgtggtc atgattacct tcgaggggta gtgctggccg tcggggaacg acctaccgcg
     3361 ggaggtgggt cgtccggata tgggttgatg atcccgagtg ggctcactct ccagtcgttc
     3421 ccggagtctc ccgtctttgc gctcggagcg gtcgcagact tgattcgaga cgctcgttcg
     3481 ggcggggcta gggtcgtctt tggcccggga ggttggatag gggcgctctt cgcagggcgt
     3541 acaggagcag cagctcgaag gcctatcgcg gaaggggtcg agtccctgcg cgtcagctgc
     3601 agggtagggt tatagatcac gctcaaagac gaactcggcg gctccgagag ggtgcgcacc
     3661 gggggtgccg acgccaagct cggggccgag ggttcacgcg gaacgagggg ctgatgcggg
     3721 agtgccgccg ccagcacgtg cagcgacccg aggactgcga ggctcaaagc gaacccagcg
     3781 ccagtcaaat accccaaacg acggtaggaa agtacgggac gggtaggagg taaagtcgag
     3841 gtggtcgaag gcgcgactgg agtgctggtc cgttgctccc ggcgcgagcg tgccaaccgg
     3901 tggtgtcgag ccgaagagac ctgattcggt gtaggaagcg taaactccac cggctgagtg
     3961 acaccgttat aatggatttg cggctcttcc gcggccggag aggaatcagt gacggacggc
     4021 tcgatggcgc aagactcgcc attcccaggg gctccgcgcg aatgcgacga atctagatct
     4081 tccaggcgcg acgctggaag actgagctgc tcgtaaaact cgggagtctg ccctcgctcc
     4141 tcgagataac gacgcagcac taaactcagg gcagccccga actcccccgc attcgtgtgt
     4201 ctcttcgcaa ccgctttgga gagcgcccgc ccgacctctg cccaagcgta cgtaggaaag
     4261 ccgggaatta ggtcggtgag cggagtcacg tgcccgtgga ccggctctac ctgtcggcgt
     4321 acgagctgaa ccactgacgg aaagctcccg tccgcgttcg cataggcatg ccgacctagg
     4381 aagagctcat aggcgatgtg accgagcgaa tatacgtccg tgcgttcgtc cacggggacc
     4441 gagtcttcac gtgataggag gtactcgggg gaggcgtaag caggggtccc gagcaaagcc
     4501 tgagaggtat tcaatgccac ctccaagccg aggcgcttcg cgatcccgaa atcgaggatg
     4561 atcggcgtct cgcccttcat tgcgatgttc tccggtttca aatcccggtg gatcaatcgt
     4621 ctttggtggg cagccccgac gccgtcgcag atcgccaaca tgtggcgcag cgcggccacg
     4681 atcggcatgg gcttgtccgg ctgtatgcgg ctgcgcaata gcccgtccgg gatcagctcc
     4741 atcgcgatcc acgcgcgtcc gtcgtgctcg ccaccgtcaa cgactttcac cacgttgggg
     4801 tgctcgatcg tcaccacgtt tcgtgcctcg cgtacgaacc ggcgacgcac tccacgtaaa
     4861 tctcggtgcc cccggcgcat gattttgagg gcacgagtta cgtccaagaa caagtgccgc
     4921 gctcgataga catccgccga accgccgcgc gcgagtagcc ccaacacctc gtaagcgccg
     4981 cacagatcac ctggcttgaa gagtggatct ggaaggtgct ggagctgatc ggcttcactt
     5041 cccgctaaga ctttccggtg attcatgctg tctccgctcg ctctgcttag gagtaaatct
     5101 gctcgcaccg gctccgaatg cctatctgct cttgagccaa gcttccgaga agggcaccgc
     5161 gctcggaaag agaaggagca acgtcacgag cgcgaagcca gcgattccga ccacgaaggc
     5221 ggcggcgccg aactgggtcg tgggccatgg tcgtccggca ttcgccgaag cgccggcggc
     5281 tggcggctga actcgcatgg cctcgtcgag cgcggccgcg aactgctcga ccgaatcgaa
     5341 acgctgtttc ggattcttct cgagcgcttg catcacgacc tcgtccaagg cggccggcac
     5401 ctgatgcagc gtccagcgcg atggcggatc ggcctgccgt aggacttgag ccctaaacac
     5461 ttcttcggtc gtcagcgcat cgtgaaaggg atcgcgtccg gcgagcatcc agtacaacac
     5521 cacgcctgcc gagtagacgt ccgagcgagc gtcgatttcc ccgagctcca cgtactctgg
     5581 agaagcggtt cgcggcgttc cccagcaaac gtcctccgga gtggagcaag ccggagtggg
     5641 cgcgttcagg ggcaagggct gagacgacaa caccttcgct tgcccgaaat ccaacaacac
     5701 cggtagcgcc tcggacgagc cgtcctcgtt cagaaacacg ttctgaggag tcaagtcccg
     5761 atgcaccatc ccgagcgagt gaaccctccc gagcgccgcg agaatccccc gcgcgatagt
     5821 gacggcccgc tcgggtgcca gagcgccact gcgcttgatt tcctcggcga gcgtgtgccc
     5881 ccagaggcgc tgaagcacca tgaacggccg cccgtcatcc aacattccta cgtcgtggac
     5941 gcgcagcacg ttcggatgat ccaactgcgc caatgcccgc ccctccacgc ggaagcgggc
     6001 tacgatatcg gcgcgctcac gcagctcgct tcgcaacacc ttgatggcca gggcgctccc
     6061 cagcgcctcg tgaacggcct cgtaaacgat gctcgtgtgc ccttcaccca aaaggccaat
     6121 caaccggtac ttgccggcaa tgacgagatt gaagggtggt tccccggagc gcatgttcag
     6181 ccaaccctcc tgcgtgactt ggtcactcgg actccgctcg cggagccctg cttgctggag
     6241 ccgcgcgcgc gtcgctgcgg ctccagcatt ccccgcgccg ctcgataaac ccgcaagata
     6301 tcctccgccg tccccggaat gctgggactt tcggtgaact cgaatgcaac ttgggcgagc
     6361 acgagccgaa tatcgttctg ctcgtagccg tcctcctgca gcgcctccat caccttacga
     6421 taggcctcga gtgggtccaa gagcacgcgt gcgggcagcg gagctggccc gcctcgcggc
     6481 gcagtccgcg gattaccgat ctgctctttg cccttctctt tgccggaggg ctccccgcgc
     6541 ttcttgcgcg ccgtcgactt ctcttccggc gcatcgatca gggggcggtg cttttcgagc
     6601 agctgagaca tgggaacttt cagcacctgc aaaagagcgt cccgcacggc gggccccact
     6661 cggttgggct gctgagccaa gtaaatcacc tgctgactca gccctccgca ggcctctccc
     6721 aatgacgctt gggtccagta tctgcgacca aactggtcac gcaaagcgag tagctcccgg
     6781 atctctgccg cgatcgctac ccgctccgct tcaggcagta ccttctctct catgtcacgt
     6841 ccagaaccct cgacgcccag gaacccagcg ccgcattgag cttcgctctc ggcgattgga
     6901 gatgcaaggg caatcttccc ggcttgctca gatttagttg ttggattgtt tatggttgta
     6961 caacaatatc cggtgggacg cgtaccggta cggatgtacc ggcaacttgg gacagcctcc
     7021 tttctcatcg agtgagaagc ggcctagtcg ctcgcgaaac ttgcgctgaa gtccgtgcag
     7081 tgaacccttc cgcggcctcg ccgaatcgag agcccgcgcg aggcacgcaa cttgcgaata
     7141 cgaggtgcga aggcacgcga ggctcgccca tgcgcggcgc gggcagtctc gggtcccgag
     7201 ccctacgctc gcaggcccga tggaaacaaa gaaatggaat ggtttgcgtc tagtggtccc
     7261 gaagccaact cggctccggc tttcttaccc ggcactcgtt ctcacgagag cacgcctgcg
     7321 taccccacgc tctgctcggc gaatgccccc agttaccaga agatgacact tcagttgtcc
     7381 tgtccgggtg gtcggaacgc aaactgtgat ccaaaaggtc cttctctaag gcttttccct
     7441 agctacactc ccacccctgg agatggcgcg cgggttggcg gtagaagcga cctggatcgg
     7501 gcgaaccgtt ggaagcgggt ggatcgtcga cagcgtgatt ggcgtgggag gctcggcgac
     7561 cgtcttccgg gcgaccaacg ccgaaggtca gagtgcagcc atcaagctga tgcatcatca
     7621 gcttgcgctg tcttggacca agcgtttcga gcgagaggcc aagctcctgc aggccttgtc
     7681 tcatccggcg attccggagc tatacgaatt cggtcgattt gggggagtcc cttttctcgt
     7741 actcgagctg ctgatggggc agtcgctgga tgtcttgtgc aaagatgcgc gtcagctcga
     7801 cgtagcccgg atcatcgcct atgcggcgga tgcactcgcg gcgctggaag tggtccacga
     7861 tcggggcatc gtgcatcggg acttgaagcc atccaaccta ctcgtcacgg atgatggcgt
     7921 gctcaaggtg ctggatttcg gagccgccgt gcacgtggag acctctttgc tagggcccga
     7981 ctcgctcacg agtgggctgc tcgggacacc cgcgtatatg cctcccgagc aggcccgcgg
     8041 tcgctgggat atggtcgatc accgctccga tatctggagc ttgggggcgg tgatctttac
     8101 gttgttgagc ggcgagcacg tgcacccggc gcagacatcg aatgagcaac tcagctttgc
     8161 aatgacgcga acggcgcgtt ctctcggacg actcgtgccg gggttgagcg cggggctcgt
     8221 gcgcgtcgtc gatcgcgcgc tcgcgtacga gcggcaagag cgcttccaat cggcggcaga
     8281 gtttcgggca gctttgctcg gatctctgcg ggacaactcc gccaccacgc gcgacgatgc
     8341 gtgtgtcacc gtgagagagt ttcgcgccgc gccgagtcac tcggcgatcg acgtgcatag
     8401 acacggtccg tcgcgcgtct tacgccgtgc tggttgggtg agtggtctgg gggcgtgtgt
     8461 cttggcggcc gcgttgattc gactcgaact ctccccaaac cgcaactcta taccttcgac
     8521 catacccgtg tccccgatct cgagtggagc cgagagaccg ccctcagctc cgggcgcttc
     8581 ccaagttccc atcctgtcct cggcacacgc agattcgcgg cccgcttccg tgatatcgtc
     8641 caacgtggcg gttcgtcgag tcgccccgta cgcttccgaa cgggcacggc tgccgctcgc
     8701 gtcagcgcgt tcggcggcgc cgagccctcg aactatcccg ccggagccgg cgccggctcc
     8761 ggtcttggcg cgttcggccc cgagcgcact agctcccgat tcggctctcg atcgtcgctt
     8821 ggggactcac ctcggcgatt cgaatggggc gagccctgcg ggagcagagg gatcgagcgc
     8881 gagcccgctc gatcttcgaa gatagtgaat ctaactttcc cgacgagatg agtccgatgt
     8941 ttcagctttg gagagtgccc gcacgtggct tccgcatcat cgcgggcttg gccttggtgt
     9001 cttccttccc ccggcccgcg ctggcggagt cgaggagccc cgttcccgac cgcgccgaga
     9061 tgccgagttc agaggcggcg ccaccgtctc atgcgcgatt tcagctcgag agtcccgagg
     9121 cctctgcctc gcaaaccacg ggagaagcta gctctaacag tcgcgcggac gggctgttcc
     9181 gcgcgggtcg cgaggcattc cgtcgagggt actacgccga ggcgtgcgca aagttcgccg
     9241 aatcggatct cctggagccg agtccaggtg caaagttgaa tcgcggcttg tgcgaggagc
     9301 agcttggaca tttcgcgctc tcctggcggc tgctccagga gagcgcgagc gcgttgcctc
     9361 ccgaagacga acgcgtgtcg attgccttgc aacacgtgca ggctttgaga ggcaaactcg
     9421 cgttcttgac cgttacgctc gcttccagcg ttccgagtac cgcgcagatc accgccgacg
     9481 gcgccgagct cggacgtgcc gagctcgggc tgcctctcgc gtatgacccg gggacccacg
     9541 tgttggagct ctctgcgccg ggttttcgtt cccggaggac cgagctgtcg cttggccctg
     9601 gcgcgaaccg cagggtaaac ctcaccctgg gcgccgcggt gcacgagccc tctggcgtgc
     9661 ccttacgggc gtcgacgact cagcatcctt ggcgcaccac atcgtttgcg ctgggaggag
     9721 cggccgtaac cctgctcatc gtgggcgcca cttcgggcgt gctggccctg aatgccaaac
     9781 acgaaatgca agcggcgtgc gatacgcaac tcgcgtgctc cgatgcgggg ttgcaggcgg
     9841 cagcccgagg tgggagttac gcggctgcaa gcaccggcgc gttcatcgga gccctcgtca
     9901 cggggggatt gggcgcagct ctttggttcg tgacgagtga atcggctcac gcgggtcgca
     9961 agagcgggtt cgaccggcga cacatgcgtt gggagttcta gctgtgaaga cactgtcatt
    10021 tcgtgttcgg caacgactgc agtcgagccg tgtatcgagt ctcgcgctct gcctcgtggt
    10081 aggcggggtg gcctgctcga atctgattgg cgccgacttc gaccaagccc atcgcgcgcc
    10141 caagaatcca tccgggggga gttcggctca gaatagcgac gcgaccggag ccagaatgtt
    10201 cggcagtggt ggtgcgtcct ccggagccgg cggtagaagc ccgcgtggag gcgccgcggc
    10261 gggaggaaca tcagagaagt cgagaccaac tgccggaggt caggctggtg caagctccgg
    10321 aagtgattcg agcgggggaa cggtcaccgt cccggagggc ggctctggcg gcgaccccac
    10381 cacctgtccc aacgctttgg agtggaagga agtcagcgag accagtatcc ccaccatggg
    10441 agaagaggcc cacgctcggt ccctgatgcc tggagttggg gtcgcgagcg gcaagagtta
    10501 ctccggttgc atcggagccc agatatcctc cgatctgttg ttgtttctgg attgcaatgt
    10561 catcgagggg gacacggtga ccttgaacga aggtgacgcg agtgaactcc ggagtgtacc
    10621 gtacaagatc acttacatgg agacgaccgt gtcgggccgc tttcgcatga acgcaatccg
    10681 tcccggggtt ccacatcagg tgccgtggag cgcccgtgcg ccgatgcgag gggaaagagt
    10741 gaccgtaatc ggcttgacgg aggaccataa gtttgccaag cgctttgctg tcactacgct
    10801 cgagcagagt agcctggagg ccgcggcgcc ttgccgcggc gactgtagtt cagcgcagga
    10861 cgatgcagcg gttctagtat tcggaagtga cgatcacctg attgcctatt gccaaacaga
    10921 tccgtgcgaa ggtcttcgac actgcgtcac gaccgccgag ttgagagcgc aatggaccac
    10981 cttctcgatg ctggcggcca tgcagcccct gttgtggggg gacgtttccg gggacggcag
    11041 agccgacgcg atcgcggttc aacgcttcaa cctcgaggtc agaacctcgc tcgggagtga
    11101 ttgggctgag cccaccaagt ggtacgactg ggagcgtgat caagattatc ccggcaagga
    11161 gcaggttgca cttggggata tcaccggtga tggtatggat gacttcgtcg cggtgggccc
    11221 cggtctccaa tacctacctt cgctcgggga tcggttcggc tcgcccgtca ttctggacaa
    11281 caagctgacg agagccgagg ccgtgagact gggggacgta gacggtgatg gttatgacga
    11341 cgtcgtgctg ctccaagcgg atactttgac cctgatgact caggttgcga cgacgcagcc
    11401 cgtggtgcgt cagtacgatg ctagggtgga gtcgggctgg ctgagcttcg atttggcgga
    11461 cgtcgacgga gactcgcatt cagacgctct cctcgcttat acggatcgca tcgaagtact
    11521 gctcggtagc agcaaaggcc tggcggcaaa tcatacgtgg ttgaacgacc tccagctcca
    11581 acctccgggg gttctgttcg cggacgttac gggtgacggc cgacccgatg ccgttcggat
    11641 cgacagcggc ggtatgtccg cattcccttc cgttggcgac cgcttcgacc ctttggcgcg
    11701 atctctcaaa ggtcttcgca tgggtcaacg aggcaatgcc tttgcggatc tagatgggga
    11761 gggacacacc gacgccgtcg tccaagacac cagcaacatg tggatattcc tttcatcgtc
    11821 aaagaacttt gggagcgccg acgattggct caatatacct tggctcggga gtcgcttctg
    11881 attcgctcgc cgtgtactcg cggtcgatcg acgagaggct atgcgtgact gacaccacca
    11941 ctctgcgcga actcctcgga gagaaaccga gctccggcga cgactcgcat cagctcgaaa
    12001 tgcacgtggt gcattctccg gacgcgagcg cgctgggacg cagattctct ctggtacccg
    12061 gtgagtccgc gctcgttggg cgtcgccagg gcctggagct cagcttggag gacgagcgca
    12121 cctcccgctc tcacgtgcgg atagtctggg atcagcgcac gcgcggctat cgctttgcag
    12181 atctgggcag tgcgaacggc acgttcctca acgggcaacg cctagagacg ggcgtcctga
    12241 gctcgaatga cgtgttgcgg gtaggggaca cgctgctcgt ttgctcgttg cctttggcgc
    12301 tcacgcgctt cgatgaagag gtgaggcgcg ccgcagcatc ttgcctgccg gtgctgcttc
    12361 tcggtgagac cgggaccggc aaagaggtgt tggccaaacg cctccacgaa cagagcggac
    12421 ggtcgggggc cttcattgcg gtaaattgcg ccgccttgcc tcgcgagctg gccacggcgg
    12481 agctgttcgg tcacacgcgc ggtgctttca gcggtgcggg taatgcgcga gatgggctga
    12541 tccgagcggc ggcaggcgga acgctctttc tcgatgagat tgctgagctt ccgatcgacg
    12601 tgcaacctac gctattgcgc tgcttgcaag agcggaagat ccggacgatc ggagccgatc
    12661 gcgaagtcga cgtggatctc cgcctcgtgg cggcgaccaa cgcggatctg gagagcctcg
    12721 tggggcaggg gaagttccga gaggatctgc tcgctcgcct agctcatatc ttgttgaagc
    12781 tcgcgccgct gcgcacgcga cgctgggaga tcctgccgct cgccaggcaa tttgcgccgg
    12841 agctcgcttt caccgccaac gcgattgaaa gcctcctgct cttcgactgg cctcgcaaca
    12901 ttcgccagct ccggtcattg attgagtctc tcgccgtgtt gaagcccagc ggtgcccgca
    12961 tccacgacgc cgacttggct gagcgcatcc ctggttttgc aaaccgcgac caggcccgcc
    13021 aggccgtcag catcacgagc gataattcga cgggcgaacg ccgaacccag ctgacggcgc
    13081 tgctccagaa gcattctggc aacatcgcga gcgtcgcgcg cgagctcggc aagccgcgtt
    13141 cccatgtcta ccgttggatg cgcaccttcc gcctgtcacg gcgacagttc gaccgctgaa
    13201 gggcccatcg cgaaccgatc gagagcttgc ttggccgcgt ctggcaatac gagctctacg
    13261 ctctggaggt tctcgcggag gtgatcgagg ctcgaggtgc cggggattag aaggatatta
    13321 gacgagcgtc gcaatagcca agcgagtgcg acctgcattg gagttgcccc gagctccttg
    13381 gcgaccctcg atagggccgt cgactgcagc ggcgagaagc cgccaagtgg aaagaagggc
    13441 acataggcga tgccggctgc ggcgaggcta tcaatcagtg cgtcgtcact gcggtgcgcg
    13501 agattgtaat ggttctgcac gcacacaatc tccgcgagct tacgggcctc ctcgacttgc
    13561 ttcgcggtga cattgctgag cccgatgtgg cggaccttgc cctcctgctg cagcttcagg
    13621 agcgcggaca gtggagcttc gatcgagccc tctgcggggc ccataacgtc gatcatgatg
    13681 cgcagattca cgacctcgag tacgtccaac ccgagatttc gaagattgtc gtggacagcg
    13741 ctcctaatat cctcggccga gaatgcaggc tcccaggagc cctgcggacc gcgcttggcc
    13801 cctactttcg taaccaggac gagatcgtcc ggataggggt gaagggcttc acggaggatt
    13861 tgattggtga tgtgcgggcc gtaaatgtcg ctcgtgtcga tgtggttgac gccactcgcg
    13921 atggcttcgc gtagcaccgc gagggcggcc tc
//
LOCUS       DASTKU010000451         4180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_85481,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000451 DASTKU010000000
VERSION     DASTKU010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85481"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VFQ61_15495"
     CDS             <1..558
                     /locus_tag="VFQ61_15495"
                     /inference="COORDINATES: protein motif:HMM:NF009566.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase subunit alpha"
                     /protein_id="HET9955914.1"
                     /translation="ELPSPLRLGLSLGASVSVDGVCQTVAGLEGELVYFDAHAETLRV
                     TTLSELREGSLVHIERSSKAGDENGGHLVSGHVDTTLELVEVVASPDNHVLVLRVPAA
                     FAKYIFNKGFVAVHGASLTVTAFDADAGTFRVYLIPETLRLTTFGQKRPGDRLNLEID
                     RQTQVLVDTLERTLRAVLPQLAKGS"
     gene            complement(722..2122)
                     /locus_tag="VFQ61_15500"
                     /pseudo
     CDS             complement(722..2122)
                     /locus_tag="VFQ61_15500"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(2115..3794)
                     /locus_tag="VFQ61_15505"
     CDS             complement(2115..3794)
                     /locus_tag="VFQ61_15505"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HET9955915.1"
                     /translation="MAKRMNVQQASVLGRHASVWAAAAILAAFASSVTVTSCGNVAPE
                     EVEFSGEEAAAQTRQALSVPVSVSFQDGVFPSADYAGTRDATLREAAGTSNFGGGASC
                     EADGDDTAGSDESCAIRWDVGGIPPGSIVQAASLTLRVTNGSADTYDIYGLTRGWEEG
                     EVSWQSAQAGTTWAAAGALGASDRGAVIGSVTGAAGTRTVSLNAAGIALVQSWVDGEA
                     NAGIVVAHATNSDGIGFGTRERATASERPRLDITYLPPETANAEIGAAPVSGEAVPTD
                     PNLKIAFVGDTATGTNFRNVLTLIKNEAAAAVVVEGDMSYSANASSWWSDVESVLGTT
                     FPVFISRGNHDDSSWSAYQAKANQHLGGATRVAGAHDANYKTTFRGLVIATIKKGDTA
                     SRINPFLSGDPHIWKICQWHQNQQALQVGSKTDEMGWAVYEACRQQGAIIQTGHEHSY
                     SRTKTLTSMTNQTVDSTCASASSLCVGAGRTFANVVGLGGNSIRPQSRCTPFTPPYGC
                     NGEWASIYTSDQGATHGAQFIVFSAGADKVATGYFKNINGQTVDTFTITHD"
BASE COUNT          803 a         1404 c         1258 g          715 t
ORIGIN      
        1 gagctcccga gccccctcag gctcgggctg agtttgggcg cgagtgtctc ggtagatggc
       61 gtgtgtcaaa ccgtcgcggg cctcgaggga gagctggtct acttcgacgc tcacgcggag
      121 actttgaggg tcaccacctt aagcgagcta cgtgagggca gcctcgtcca tatcgagcgt
      181 tcgagcaagg ccggcgacga gaacggcgga cacttggtct ccggtcatgt cgatacgacc
      241 ctggagctcg tggaggtcgt ggccagccct gacaaccacg tcctagtcct gcgagtccca
      301 gccgctttcg ccaagtacat cttcaacaag ggtttcgtcg cagtccatgg cgcgagcctt
      361 accgtaacgg cgttcgacgc ggacgccggc accttccgcg tctacctcat tcccgagacg
      421 ctgcgcttga cgacgtttgg gcagaagcgc ccaggggacc gattgaatct ggagattgat
      481 cgacagactc aggtgctcgt agatactcta gaacgcacgt tacgcgcggt tctccctcag
      541 ttggcgaagg gttcttgagc tgagcgagcg cgcgggcgag aaccagcttg gacactggcc
      601 aagcatcgcg gagtcgagct tccggagcga gcaggcgcgc cgcttcgcac gcatcgccct
      661 cctcgagcgc gagtaccgcg aggttgtgct gtagttgacg cttcagtcca gtgtcgtctg
      721 tccgctgcag cgctcgacgg aaggtgcgtc gcgcgagttc gtggcgccca agctcgaggt
      781 aggccacgcc gagattctga agagtggcgc caagatcgac gtacgacagg tagcccgggg
      841 ggtccaccgc ggcggtttcg tagtgacgtg cggcctcgtc ccagcgtccc gccagacgcg
      901 catgctcacc caagtagaag tcggcctcgc gacacagcgg tgatcgctgc ttctcacctc
      961 gccacaaaac gacatcactc gcgaaccgcg cgcttcgcat ccaggccaac ggcgcaaacg
     1021 aaacgcaaac cagaacgatg gcgaaagcaa atcttcgcga ggcccgcccc ccgcggcaac
     1081 gtcgctcgag ggttcgagcc acgagcatgc acacgaacac gagcggcagg tagagatagt
     1141 ggggagacca ccagcgcatc actggaacga tctgtaaaca cggtagcacg ctagacccca
     1201 gcaacagccc gagcacaccg ccgcggatcg caagccaccc gagcgctccc aacaccagcg
     1261 cacccatgag tgccggccca ctccacaccg ttagcaccgg aaaggtgtcg cacaccgtcg
     1321 agtccaacgg taggagcacg gccagagcgc tcttgctcaa ggcgcctagc ctggttccga
     1381 ccgctgaatc ccacgagagt gacagatgag aggcgcggaa cgctggggca taccggattc
     1441 gcagcagcgt aacgagcaac agtgcgccga gctcacacag ccaaagggcc catgggcgcc
     1501 gaacccgcat gggctcgtgg ttcaccgtcc tgcgtggagt cacccaagcg ccgcgttcca
     1561 aagtgacaat ccaaacgggc gcgagaaccc aaccggtttc cttgctcaat cccgcgccca
     1621 ctatgcacag ggcggccaga gtctcgtttt gccttcgatg ggcgatcagc aaacccatca
     1681 gcgacgccgt cagcatcgac tcggagcgaa aggccgtggc tgacgccacg aggctcgtga
     1741 ttggatgcgt cgcaaacaga gcccctgccc ccaaagcggc cggcgccgta agccccagcg
     1801 cgcgagccgc cgccgcaacc gaaactgccg ccaacgcatg ccaaagcaac gtcacggcgt
     1861 gataccagat cgccgtatga ctcacggctg cgtccatgga tagggccagc gccatcaacg
     1921 gacgatagaa ccctgtcccc gcaaagccgt gcgagaacgc tttccagacc tcttggagcg
     1981 ggagcagcgc gagcccttgc tcgaggtgcg catgatcgag ccaaatgaac ccgcccccca
     2041 gggcggtggc gtgagatgcg acggcgatcg ccgcgacgaa cgagagctcg gctcggccga
     2101 gccgccccgg ctcttcaatc atgcgtgatg gtgaaggtgt ctaccgtctg tccgtttatg
     2161 ttcttgaagt atcccgtcgc caccttatcg gcacccgcac tgaacacgat gaactgcgcg
     2221 ccgtgagtgg ccccctggtc cgaggtatag atcgacgccc actcgccgtt acagccgtaa
     2281 ggcggcgtga acggcgtgca ccgggactgg ggacggatgc tgtttccgcc tagcccgacc
     2341 acgttggcga acgttcgacc tgctcctacg cacaagctcg aggcgctcgc gcaagtcgaa
     2401 tccaccgttt gattggtcat gctggttagg gtcttggttc gcgagtaact gtgctcgtgc
     2461 ccggtctgga tgatcgcgcc ctgttgtcgg caagcttcgt agacggccca gcccatctcg
     2521 tcggtcttac tgccgacctg cagcgcctgt tgattctggt gccattggca gatcttccag
     2581 atgtgcgggt cgccgctcag aaacggattg atgcgagacg ccgtatcgcc cttcttgatg
     2641 gtagcgatca ccaggccgcg aaaggtggtc ttgtagttcg cgtcgtgcgc tccggcaacg
     2701 cgcgtcgcgc caccgaggtg ctggtttgct ttagcctgat aggcgctcca ggagctgtca
     2761 tcgtgattgc cccgcgaaat gaagaccggg aaagtggtac ccagcacgct ttccacatca
     2821 ctccaccagc tgctcgcgtt ggccgaatag gacatgtccc cctcgacgac caccgccgcc
     2881 gcggcctcgt tcttgataag ggtcaacacg ttgcgaaagt tggtgccggt cgcggtatca
     2941 ccgacgaacg caatcttgag gttcggatcg gtgggtaccg cttcgccgct caccggcgcg
     3001 gcgccaatct cggcgttcgc agtctccggg ggtaagtaag tgatgtcgag ccgcgggcgt
     3061 tcgctggcgg tcgcccgttc gcgcgtcccg aagccaatgc catccgagtt cgtggcgtgc
     3121 gcgaccacga tgcctgcgtt cgcctcacca tccacccaag actgcaccag agcgatgccc
     3181 gccgcgttca agctgacggt gcgggtcccg gccgcccccg tcacgctgcc aatcaccgcg
     3241 ccgcggtcgg acgcgcctaa agcgcccgca gctgcccaag tcgtacctgc ttgggcactt
     3301 tgccagctga cctccccctc ctcccagcca cgcgtcagtc cgtagatatc gtaagtgtcc
     3361 gcacttccgt tcgtcacacg caacgtgagt gacgccgctt gcacgatgct gcccggggga
     3421 atgccgccga cgtcccagcg gatggcgcag gactcgtcgg agccggccgt gtcgtcacca
     3481 tctgcttcgc agctcgctcc gccgccgaag ttgctcgtcc ctgcagcctc tctcagcgtg
     3541 gcatcacgag ttccggcgta gtctgcgctc gggaaaacac catcttggaa cgaaacgctg
     3601 actggcacgg agagcgcttg ccgggtctgc gcggctgcct cctctcccga aaattcgact
     3661 tcctccgggg ccacattgcc gcaggaagtg acggtcaccg atgacgcaaa cgccgccaaa
     3721 atcgcggccg ctgcccacac tgacgcatgc cgccccaaca ccgatgcttg ttgcacgttc
     3781 atcctcttcg ccatgatcgc acctctctcg gacgggcctc cctcgaccga tgcggcggca
     3841 tcctcggctc ctccaatgac gcgagccgca cgccatcgtt acgacttggc aaatggtccg
     3901 tggccgctca ggtctcgaat cacgcgaggt gcgaccgcca ccgcgaaccc caaatcgttc
     3961 gccggcccct cgtacgggcg agagctcgac agatcaaacc ggaccgctga gaaatcaaag
     4021 agaacggcat gaccccacac gctgggcccg cggtgcaaag gcccactgga acgcttccta
     4081 gcgaaagcgt cccgggaaac gtccacatcg agaaggggcg tttgttctag cgctgagagc
     4141 agctcgaaga tcagccgggc aacgctcgac aagcggcccg
//
LOCUS       DASTKU010000452         4450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_85495,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000452 DASTKU010000000
VERSION     DASTKU010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85495"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFQ61_15510"
     CDS             <1..499
                     /locus_tag="VFQ61_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955916.1"
                     /translation="FGGESASFHTRIEEGLSLPEMADFASRVEQRARELDPGSNLTAA
                     QIGARAEFGATCVGCHHRLDRPLSRDLGQNLTLPVVDIPPGDQDIKSFTQVNNREFEP
                     CAANGTDQSQSCYLLSPALREIFLPKRKETLERYLQFPGTFGPLPNGQSSTKTVSGTP
                     VTQSN"
     gene            707..1690
                     /locus_tag="VFQ61_15515"
     CDS             707..1690
                     /locus_tag="VFQ61_15515"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9955917.1"
                     /translation="MQTRRLGSFEVSALGLGCMGMSEFYGSRDDAESIATIHRAIELG
                     VTLLDTADMYGPYTNEELVGRAIRGKRGQVQIATKFGIVRGPRPEDRSVQGSPAYVRE
                     AVEGSLRRLNVEVIDLYQYHRVDIRVPIADTVGAMAALVREGKVRHIGLSEVSATTLR
                     QAHAVHPITSVQSEYSLWTRDPEHDGVLQACRELGIGFLAYSPLGRGFLTGQIRRFED
                     FEADDYRRHSPRFQGENFQKNLDLVQRIATLAEQKGCTPSQLALAWTMARHSFVVPIP
                     GTKRRKYLEENAGALQVRLSPEELAQIDSIFPPGTASGTRYPEQMMQTLGR"
     gene            complement(1799..2368)
                     /locus_tag="VFQ61_15520"
     CDS             complement(1799..2368)
                     /locus_tag="VFQ61_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955918.1"
                     /translation="MQIRSFYGWLVFAALAAGCAADAGDPQPDAVSPGSVPADQAASS
                     EPDSDAAHSDSQTLCIGFPEVATWYRYSGGGPYRIDVHYGGGDCDLHATVWEKQLDGS
                     LYLRGDFAAHWQLLADGTHWAVVDPVNVGGYVEHLWLDAATDSTSVRVWIYYESLDSK
                     PDAVEDTTYYRLRPPSDIFNPRRPISIGP"
     gene            complement(2628..3185)
                     /locus_tag="VFQ61_15525"
     CDS             complement(2628..3185)
                     /locus_tag="VFQ61_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955919.1"
                     /translation="MNVSWINGLWVRALGLVVLGMNGGCSVETEPEIQRSAVQCGNTL
                     TCSSSEMCVVTEAIGPYVEQPNQFSCVPIPSACLGRDVCGCEDMDGAVQKPRIACSSE
                     RLRVGGVTCGSRSCEPSEYCLATYRAQGAAEPFERQCFATPAACQSDVCAAGCAEAIE
                     QDHPGQVVWGCFTSPFAQAVRLGPG"
     gene            complement(3476..3925)
                     /locus_tag="VFQ61_15530"
     CDS             complement(3476..3925)
                     /locus_tag="VFQ61_15530"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET9955920.1"
                     /translation="MKAEGGCLCGSIRYEVEGEPLITATCYCRDCQYAAGGGPGYAAS
                     FLREKVRITRGEPVTFESESSAKNRVQRQFCGRCGTPLFSNSSAAPMLLTVKVGSLDD
                     PSEFRSMGSIWLKSAQPYHRPDKEQRCWDTVPTQQEMNEFLAKRLAR"
     gene            4021..>4450
                     /locus_tag="VFQ61_15535"
     CDS             4021..>4450
                     /locus_tag="VFQ61_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955921.1"
                     /translation="MAKEPLSLSGVSVAGIETSIEVPSLKLVLDMGRCSQTAINQKWV
                     LVSHGHLDHIGAIAQHAARRALMKMSEGIYVVPASIHPVVERLFNAAGELDGHPIPRT
                     VVPLEPGQELALSGGRYVRPFATYHRVPSQGYAVWQSRNRL"
BASE COUNT          890 a         1431 c         1326 g          803 t
ORIGIN      
        1 ctttggaggg gagagcgcga gctttcacac tcgcatcgag gagggcctaa gcctgcccga
       61 aatggcggac ttcgctagtc gcgtcgagca aagagcgcga gagctcgatc cgggctcgaa
      121 tttgaccgcg gcgcagatcg gagcgcgagc cgagttcgga gcgacttgcg tgggctgcca
      181 tcaccggctc gatcggccac tatctcggga tctgggacag aacctcacct tgccggtcgt
      241 agacattccg cccggtgacc aagacatcaa gagcttcact caggtgaaca atcgcgagtt
      301 cgaaccgtgc gcggcgaacg gaacggacca gagtcagtcc tgttacctct tgtctcccgc
      361 gctcagggag atcttcctac cgaaacgaaa ggagaccctc gaaaggtacc tgcagttccc
      421 gggaacgttc ggaccgctcc cgaacgggca gagctctacg aagaccgtca gcggtacgcc
      481 cgtcacccag tccaactaag ggcaaaccga ttgagctcgg agcggaacgt cgggcgagct
      541 tgggctaggc gcgcggctcg ggtcaatttg cgcagccgct cgacgtccgt tctctcgctc
      601 tttccccaac tcccttgacg caaaatcttt gcactcgaca ttggaaccga gagcttgacg
      661 acgcgcggtt cccgaatcgt aatgggggcg gccaaaggag aaaccgatgc aaactcgtcg
      721 cttgggctcg ttcgaggtct ctgcgctggg actcggctgc atgggaatga gtgagtttta
      781 cggatcgcgc gatgatgcgg agtccattgc gaccattcac cgcgccatcg agctcggggt
      841 caccctgctc gacacggcgg acatgtacgg gccctatacg aacgaagagc tagtgggccg
      901 cgctattcga ggcaaacgtg gccaggtgca aatcgccacg aagttcggca tcgtacgcgg
      961 cccacgcccc gaggatagaa gtgtgcaggg cagcccagcc tacgtccgcg aagcggtcga
     1021 gggcagcctt cgacgactca acgtcgaggt gatcgatcta tatcagtatc accgcgtcga
     1081 catcagagtt cccatcgcgg acacggttgg agccatggcg gcgttggtgc gagaaggcaa
     1141 ggtgcgccat atcggcttgt ccgaggtcag cgcgacgacg ctgcggcaag cccacgcagt
     1201 gcacccgatt acttccgtgc aatccgagta ttcgctttgg acgcgcgatc cggagcacga
     1261 tggcgtgctg caggcctgtc gcgaactcgg tatcggcttc cttgcgtata gcccgctcgg
     1321 tcgcggcttt ctcaccggtc agatccgccg cttcgaggac ttcgaagcgg atgactatcg
     1381 acgacattcc ccccgctttc agggcgagaa ctttcaaaag aatctagact tggtgcagcg
     1441 cattgcgacc ctcgccgaac agaagggctg caccccgtcc cagctcgcgc tggcttggac
     1501 catggcgcgc cattcattcg tggttccgat cccggggacc aagcggcgca agtacctcga
     1561 ggaaaacgcg ggcgcactac aagtgcggtt gagccccgag gagctcgctc aaatcgactc
     1621 catcttcccg cccggcacgg cttcgggcac acgttatcca gaacagatga tgcagacgct
     1681 cgggcgttga gccccaccgg ataaatctcg gagccagcgt tcgacccggc tccgccgccg
     1741 gagaggccaa cgcctccggg caaagcgccc gcgttgtgag aacgttggcc gcgcgaagct
     1801 acggaccgat cgagatgggg cgtcgcggat tgaagatatc ggacggtggg cggaggcggt
     1861 agtacgtggt gtcctcgacg gcgtccggct tactgtcgag cgattcatag tagatccaga
     1921 cgcgcacgga cgttgagtcc gtggccgcat cgagccagag atgctcgaca tacccgccaa
     1981 cgttcaccgg atccactacc gcccagtgcg tcccgtcggc aagcagctgc cagtgcgctg
     2041 caaagtcacc tcgcagataa agcgacccgt ccagctgctt ctcccagacc gttgcatgca
     2101 ggtcgcagtc acctccaccg tagtggacgt cgatgcgata tggccctccc ccgctgtagc
     2161 ggtaccaggt tgcaacctcg ggaaaaccga tacacagcgt ctgactatcc gaatgagcgg
     2221 cgtccgagtc cggctcactg gatgcggctt gatccgccgg cacggaaccc gggctaacgg
     2281 catcgggctg tggatctccg gcgtccgccg cgcacccggc cgcaagcgcc gcaaacacca
     2341 gccaaccata gaacgatcga atctgcatga tgcatctcct ccaattgggt tctccctggg
     2401 gatgcagagc aaggtgtgca gtcgcatgcg atgaagtggt cattgattcg actggtggcg
     2461 tgcgttaccg agacaaccac cttcataatc gacccggaag aagggctctg ccggccctgc
     2521 ccgcgcatcg cgagctcggc ttctcgcaac aggaggagca cgtcagcccg cgtcgacagg
     2581 tttgatgacg atccggtcag cttgcgggcg cgtcgtccac cactccgtca acccgggcca
     2641 aggcgcacgg cctgggcgaa ggggctggtg aaacaacccc acaccacttg gccagggtga
     2701 tcctgctcaa tggcctccgc gcaacctgcc gcacaaacgt cggactgaca tgccgcaggc
     2761 gtggcaaagc actgtcgctc gaagggctcg gccgccccct gcgcacggta tgtcgccaga
     2821 caatactcac tcggttcgca gctccgcgag ccgcacgtca caccgcccac gcgcaggcgc
     2881 tcggagctgc aagcaatcct gggcttctga accgccccgt ccatgtcttc gcatccgcag
     2941 acgtcccgcc caagacaggc gctcggaatt gggacacacg agaattgatt gggctgctcg
     3001 acgtacggtc cgattgcctc ggtgaccacg cacatctcag agctcgagca agtcagcgtg
     3061 ttgccacact gcaccgctga cctctgtatc tccggctccg tctcgacact acagcccccg
     3121 ttcatgccta gcacaacgag gccaagcgcc cgcacccata ggccattgat ccacgatacg
     3181 ttcacgatcc tccgcatgaa ctcgttacga agcaccgcat gtgccgatga cgatttgttc
     3241 gcgaaaatcc gcattcggag ccgcgagtgt accccgacgt tcacagggct gtaatcaaag
     3301 gcgtggggag tctgcaaggc gctttgggtc ttttgggtag caggcggcac aagatctgaa
     3361 ccggcgcttg aacttggcac cggatgcccg aaccaagttg gaactgacac ccagtggagt
     3421 cgattctcgc tcggccaccg ccgattccgc ccgcctcggg cccccttgat aacactcacc
     3481 gcgcgagtct cttcgctaga aactcgttca tttcctgctg agtggggacg gtgtcccagc
     3541 aacgttgctc cttgtcgggc cggtgatagg gctgcgcaga tttgagccag atcgaaccca
     3601 tggaacgaaa ctcgctcggg tcgtccagag agcctacttt aaccgtgagc aacatgggcg
     3661 ccgcgctcga gttcgagaac aagggggttc cgcaccgccc acagaactgt cgttgaacgc
     3721 ggttctttgc gctcgattcg ctttcgaacg tcacgggctc accgcgcgtg atccgaactt
     3781 tctcacgcaa gaaggacgcc gcgtagcccg gcccgccgcc agccgcgtac tggcaatccc
     3841 tacagtaaca ggtggccgtg atcaacggct cgccctccac ctcgtaacga atcgaaccgc
     3901 acaaacatcc gccttctgct ttcatgttga tctcctgatg gttgagtgca cttcgcgacg
     3961 atgagggagg tgcgcacgcc agcttgcgag aatcgggccc atctcgcaag gtacaggttc
     4021 atggccaaag agccgctgtc actctccggc gtcagtgtag ccggcatcga gacctcgatt
     4081 gaagttccta gcctgaagct cgtgctggat atgggtcgct gctcgcagac ggcgataaac
     4141 caaaaatggg ttctggtgag ccacgggcac ctcgaccaca tcggggccat tgcgcaacac
     4201 gccgctcggc gcgccctgat gaaaatgtcg gagggcatct acgtggtccc cgctagcatt
     4261 caccccgtcg tcgagaggct gttcaacgcc gcgggggaac tcgacggaca ccccattccc
     4321 cgcacggtgg tcccgctcga gcccggccag gagcttgctc tatccggagg gcgctacgtg
     4381 cggccgttcg cgacctatca ccgggtcccc agccaaggtt acgcggtatg gcagagccgg
     4441 aatcgcttgc
//
LOCUS       DASTKU010000453         5630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_753799, whole genome shotgun sequence.
ACCESSION   DASTKU010000453 DASTKU010000000
VERSION     DASTKU010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753799"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /gene="ureC"
                     /locus_tag="VFQ61_15540"
     CDS             complement(<1..1364)
                     /gene="ureC"
                     /locus_tag="VFQ61_15540"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HET9955922.1"
                     /translation="MSRLGRRAYAEMYGPTRGDRLRLGDTSLIIEVEADHTVFGEEVK
                     FGGGKVIRDGMGQSQRGNSEAMDTVITNAVILDASGIIKADIGISAGRIAAIGKAGNP
                     DIQPGVNIVIGPGTEIIAGEGLIATAGGIDSHIHFICPQQCEVALMSGVTTMLGGGTG
                     PAAGTNATTCTPGPWNIARMLQALEAFPLNFGLLGKGNASLPGALIEQVEAGVCGLKL
                     HEDWGTTPAAIDACLSVAEQYDIQVAIHTDTLNESGFLEDTLAAFKGRTIHTFHTEGA
                     GGGHAPDIIRACGAPNVLPSSTNPTRPYTRNTVDEHLDMLMVCHHLDPSIAEDVAFAE
                     SRIRRETIAAEDILHDLGAFSMMSSDSQAMGRVGEVVIRTWQTAHKMKLQRGPLPQDS
                     DRNDNLRAKRYVAKYTINPAITHGIAHEVGSITVSKLADIVLWKPAFFGVKPALVIKG
                     GLIVA"
     gene            complement(1361..2065)
                     /locus_tag="VFQ61_15545"
     CDS             complement(1361..2065)
                     /locus_tag="VFQ61_15545"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HET9955923.1"
                     /translation="MDLSPQEKDKLLLFTAALLAERRRARGLKLNYPEAVAYISAAVL
                     EGARDGKTVAELMTYGTQLLGADDVMEGVAELIPEVQVEATFPDGSKLVTIHHPIPAR
                     GTLVPGELLPHESAPEHELNAGRELRVLAVKNTGDRPVQVGSHYHFYEVNPALTFERS
                     QARGFRLDIPAGTAVRFEPGQTRTVTLCALSGDRKVYGFRGDVMGGLDDDGPDTVKRP
                     SSPDDPSDTSGAGGQP"
     gene            2895..4388
                     /locus_tag="VFQ61_15550"
     CDS             2895..4388
                     /locus_tag="VFQ61_15550"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9955924.1"
                     /translation="MRYPFIALATLGGACATSLSWAQTVGVSGGAATSANGASPPPAV
                     NSPTVPPAEMIAAPPQAPSPPAPAAEASEEARVAPVSLGVFGGMFFPSSSHALLERAP
                     QESYGSTIPELGGRLGIYPLPYLGVEVEGATMPTETKSGSKASLWAARASAVVQYPGT
                     LTPFLLVGGGALGAGSNTMGTDVDPALHFGLGAKFLLDNFLTLRFDARDNLTQKWEAS
                     QGTQTHHPELLLGLSFELGPAKQTAAAPISAAPLDSDGDGLPDDKDACPSEAASQPDG
                     CPLRDADNDGVFDNKDACPNDAGKAPCGCPVQDYDGDLVIDELDKCPKEFGPIQGCPD
                     PDPDRDAITGAADRCPDKPETKNGFQDADGCPDDLPETVKKFTGVIKGIEFDRAKDTI
                     RPVSTQVLDAAVKVLAEYPNLRVQVTGHTDSDGDRQTNVELSKRRADAVKSYFAGKGV
                     DPTRIETRGAGPDEPIADNKTAEGKQKNRRIEFKLIDEVKSSERGQP"
     gene            4409..5281
                     /locus_tag="VFQ61_15555"
     CDS             4409..5281
                     /locus_tag="VFQ61_15555"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HET9955925.1"
                     /translation="MISLLALGAGISLSPDALAQEAPKGLMGAKTATQGKEDVAVSGF
                     AAPVGKLEESKDTTELKISLGGLFASGNSKSIAANGTTKLHVRRDDNQLDAAFAANYG
                     RAAVQGETAMRPTVENFQGRMRYDRFVASNVALFLSMSALKDRFQGLDLRLNLDPGLA
                     YYFLDVAKHRFWAEGGYDLQSDFRRESAINDALAKGEVLDRSEVRHNLRLFLGYENAL
                     NKQLTFDTGVEYLQALKETKNYRINWNAGLTSQVSGNFSIATTLNLRYDHNPLPNIKN
                     TDVVTAISLVYQLL"
     gene            complement(5381..>5630)
                     /locus_tag="VFQ61_15560"
     CDS             complement(5381..>5630)
                     /locus_tag="VFQ61_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955926.1"
                     /translation="SAGTLLEHWKAGELEELSPIGMTLVAEGRPKGEFVPVHLRASVT
                     EIGTLLLEAIPTAPVEPDERFKIELSVRGASSPEEPPA"
BASE COUNT         1092 a         1781 c         1717 g         1040 t
ORIGIN      
        1 gccacgatca agccgccctt gatcaccaac gcgggtttca cgccgaagaa ggccggtttc
       61 cagagcacga tgtccgcgag cttcgacacc gtgatagatc cgacctcgtg cgcgataccg
      121 tgcgtgatgg cggggttgat cgtgtatttg gcgacataac gcttggcccg caggttgtcg
      181 ttccggtcac tgtcttgggg tagtggaccg cgctgcagct tcatcttgtg agcggtttgc
      241 caagtccgga tcaccacttc acccacgcga cccatggcct gcgaatcact ggacatcatg
      301 ctgaacgcgc cgaggtcgtg cagaatgtcc tcggccgcga tggtctcccg acgaatgcgg
      361 ctctccgcga acgcgacatc ctcggcaatg ctgggatcca agtgatgaca caccatcagc
      421 atatcgaggt gttcatccac tgtattccga gtgtaaggcc gagttggatt cgtcgaggac
      481 ggcagtacgt tgggagcgcc gcaagcacga atgatgtctg gcgcgtggcc gccaccggca
      541 ccttcggtgt ggaaggtgtg gatcgtgcga cctttgaacg cggccagcgt gtcttcgaga
      601 aagcctgact cattcaaggt gtcggtgtga atcgcgactt ggatatcgta ttgttcggcc
      661 acactcaggc aggcgtcgat ggccgcggga gtggtgcccc agtcttcgtg cagcttcaga
      721 ccgcagacac cggcttcgac ttgctcgatc aaagccccag gcaggctggc gttgcctttc
      781 ccgagtagcc caaaattcag aggaaaggcc tccagggctt gcagcatccg agcaatattc
      841 caagggcccg gcgtacaggt agtggcgttg gttcccgctg ccggtcccgt gcctccgccc
      901 agcatggtgg tcacgcccga catgagcgcg acttcgcact gctgcggaca aatgaaatgg
      961 atgtgcgaat cgatgccccc ggcggtcgcg atcaagcctt caccggcgat gatctcggta
     1021 cctggcccaa tcacgatgtt gacgcccggt tggatgtccg ggtttccggc cttaccaatc
     1081 gcggcgatgc gcccagcgct gattccgatg tccgccttga tgatacccga ggcgtcgaga
     1141 atgacggcgt tcgtgatcac ggtatccatg gcttccgaat tgccacgttg gctttgaccc
     1201 atgccgtccc ggatcacctt gccgccgccg aacttcacct cttcgccgaa tacggtgtga
     1261 tcggcctcga cctcgatgat caaactggtg tcgccgagac gcagacgatc tccccgcgtc
     1321 ggtccgtaca tctccgcgta ggcgcggcgt cccaggcggc tcatggctgc cctcccgcac
     1381 cagaagtatc gctggggtcg tcgggtgagc tcgggcgctt caccgtgtcc gggccatcgt
     1441 catccagtcc gcccatcacg tcacctcgga agccgtagac cttgcgatcc ccgctgagtg
     1501 cgcaaagcgt gacggttcgg gtctgaccgg gctcgaagcg caccgccgta ccggccggga
     1561 tgtcgagcct gaagccgcgc gcttgtgagc gctcgaaagt gagcgccggg ttcacctcgt
     1621 agaagtggta gtgagacccc acctgcaccg ggcgatcgcc ggtgttcttc acggccaaca
     1681 cgcgcagctc gcggcccgcg ttcagctcgt gctcgggcgc ggattcgtgc ggtagcagct
     1741 cgccgggtac gagcgttccc cgagcaggaa ttggatgatg aatggtgacc aacttggatc
     1801 catcgggaaa tgtggcctcc acttgaacct ccggaatcag ctcggcgacg ccctccatca
     1861 cgtcgtccgc gccgagcagc tgcgtaccgt aagtcatgag ctccgccact gtcttgccgt
     1921 cgcgggcccc ctccagcacg gcggcgctaa tgtatgcgac cgcctccgga tagttgagct
     1981 ttagcccgcg cgcgcggcga cgctcggcga gcagagctgc agtgaacagc aacagcttgt
     2041 ccttctcttg gggcgagaga tccatatcag ttctcgataa gcctcgatcg gcgttgccgt
     2101 aaagccctaa accgccttgc cgggcgtgag gatgtccgga gagcgagtcg tccaagactt
     2161 gccgggggac cgcccaaggc tccgcgagta ggcccggagg cgggaggcga gcatcgaggc
     2221 gacagtcagg tacccgatac cctaccgaaa cgcgatctcg ggcaaacttc cagcgcggcg
     2281 cggggtcgac gcgggcgcaa ccgcgccccc cgttcactct agacagtgtt caccctggac
     2341 agtgttcaga cagtggagag ccgcgcacgc gcgcgcctcg gcgcttagac gctcaccagc
     2401 tcggcagtcg ccagctcggc cgcagcgcgg cgatccggtt cgcaaagcgc ggctcgcaat
     2461 tgagtgtgac aggcgtcacc ctctccccga cactcgctgc cgagcagctc gtcacgcgcg
     2521 ttcaggtcac gtcggtttcc gacttctcga gccagtttgc ggcgaaactg tcgctcgtcg
     2581 aagcgcgctt ttcaacgcac tctcggtcgc cgcacgtcga aagaccgagt gcaaagccat
     2641 tggcgtgacc gcacttcagt aagtctggcg cagacttgac gtctacgggg caagcgtccg
     2701 atactcgcgc cgaaagcaaa ggcatttcgc tggggaaccg aataccgata tctgctttct
     2761 ctcgatcttc aacatcgaat gtttgtagga acgaataact gtatagcgag caaccgatca
     2821 agcattcaac aacgtcgaat gagctttatg ccttatttca tggcttattc tttcataccc
     2881 tataaggtgc gacgatgaga tatccgttca tcgcattggc cacactcggt ggagcgtgcg
     2941 cgacgagcct atcgtgggcg cagacggtcg gcgtgagtgg tggcgctgcg accagcgcga
     3001 acggcgcgtc acctcccccg gccgtcaact cccccaccgt gccccctgcc gaaatgattg
     3061 cggcgccgcc gcaagcccca agcccgccag cgccggccgc ggaggcctcg gaagaggcgc
     3121 gtgttgcgcc cgtttccctc ggtgtattcg gcgggatgtt ctttccgtcg agttctcacg
     3181 cgctcttgga gagggccccc caagagagct acggctcgac gattcctgaa ttgggtggac
     3241 gcctggggat ctatcctttg ccgtacttgg gggtggaggt ggagggcgcg accatgccca
     3301 ccgaaacgaa gagcggaagc aaggcgagtc tatgggcggc acgtgcttcg gcggtcgtcc
     3361 agtatccggg caccctcact ccgttcttgt tggttggtgg tggggccctc ggcgccggca
     3421 gcaacaccat gggaacggac gttgatccgg cgctgcactt cgggctcggc gccaagttct
     3481 tgctcgataa ctttctcacg ttgcgcttcg acgcacgcga taacctgacc caaaagtggg
     3541 aagcatcgca aggcacgcaa actcatcatc cggagttgct gctcgggttg agcttcgagc
     3601 tgggcccggc caagcaaacg gccgcagcgc ccatctccgc ggcgcccctg gatagcgacg
     3661 gcgacggctt gcccgatgac aaagatgctt gtccgtccga agccgcctcc caaccagacg
     3721 gttgcccgct ccgagacgcg gacaacgacg gagtgttcga caacaaggac gcttgcccca
     3781 acgacgcagg taaggcgcct tgtgggtgcc ccgtgcaaga ttacgacggc gatttggtga
     3841 tcgacgagct ggacaagtgc cccaaagagt tcgggcccat tcaaggctgc cccgatccgg
     3901 atccggaccg tgacgcgatc acgggagccg ccgaccgttg tcccgataag cccgagacca
     3961 agaatggctt ccaagacgcc gacggctgcc ccgacgactt acccgagacg gtcaaaaaat
     4021 tcacgggcgt aatcaagggc atcgaattcg accgcgcgaa agatacgatt cgtccggtct
     4081 ccacccaagt tttggacgcg gccgtgaagg tcttggccga gtatcccaat cttcgagtgc
     4141 aggtcacggg tcacaccgat tcggatggcg accggcaaac caatgtcgag ctctcgaagc
     4201 gacgcgctga tgccgtaaag agctactttg ccggcaaagg cgtggacccc acgcgcatcg
     4261 aaacccgcgg ggccggcccg gatgagccga tcgcagacaa caagacagcc gaaggcaagc
     4321 agaagaatcg tcgtatcgag ttcaagctca tcgacgaggt caagagctcc gagcgaggtc
     4381 agccgtgaag cgcaccgtca tatcaagcat gatttcttta ctggcgctgg gcgcgggcat
     4441 ttcgctcagc cctgacgcgc tcgcccaaga agcgccgaaa ggcttgatgg gagccaagac
     4501 cgcgacgcag ggcaaggaag acgtggccgt ctcgggcttt gcggctcccg tgggcaagct
     4561 cgaagagagc aaagacacga cggagctcaa gatcagcttg ggcggcctgt ttgcgtcggg
     4621 caactcgaag tcgatcgccg ccaacggcac caccaagttg cacgtgcgac gcgatgacaa
     4681 ccagctcgat gccgcgtttg cggccaacta cgggcgcgcc gccgtgcagg gcgagaccgc
     4741 catgcgaccg accgtggaga acttccaagg tcgaatgcgc tatgaccggt tcgtcgcctc
     4801 caacgttgcg ctgttcctct cgatgtccgc cttgaaggac cgttttcaag gcttggactt
     4861 acgcctgaac ttggacccag gcctcgccta ctacttcctc gatgtggcga aacaccggtt
     4921 ctgggccgag ggcggctacg atctgcagag cgacttccgc cgcgagagtg cgatcaatga
     4981 cgcgctcgcc aagggcgaag tactcgaccg ctccgaggtg cgccacaacc tgcgcctctt
     5041 cttgggctac gaaaacgcgc tgaataagca gctgacgttc gataccggag tcgagtatct
     5101 gcaggcgctc aaggagacca agaactaccg catcaattgg aacgcggggt tgacctcgca
     5161 ggtgtcgggc aacttctcga tcgcgaccac tctcaaccta cgttacgatc acaacccgct
     5221 gccaaatatc aagaacacgg acgtggtcac ggcgatcagc ctcgtttatc aactgctcta
     5281 atcgctgttt gcccgagcca tagagctcgg gatcccccgc gactctggaa aaactcgggc
     5341 ccggacggcg catgccggca tccgggcccg aatcgcgaga tcaagcgggc ggctcctcgg
     5401 gagaagacgc cccgcgcacg ctgagctcga ttttgaagcg ttcatcgggc tccacgggag
     5461 cggtggggat cgcctccagc aacagggtgc caatctcggt aacgctcgcg cgtaggtgca
     5521 cgggcacgaa ctcgcccttt ggacggcctt cggccaccag ggtcatgccg atcggtgaca
     5581 gctcctcgag ctcaccagct ttccaatgtt cgagcagcgt ccccgcggaa
//
LOCUS       DASTKU010000454         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_753969, whole genome shotgun sequence.
ACCESSION   DASTKU010000454 DASTKU010000000
VERSION     DASTKU010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753969"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="VFQ61_15565"
     CDS             <1..1240
                     /locus_tag="VFQ61_15565"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HET9955927.1"
                     /translation="ASQSNPVQGGAVQGGAVQGGGLSGAPSASNQGPQEMPSLAPSPL
                     ITERRPTSSAFSGQASAEGSTQVSAQDRDPRALSDFRPSLDPYGSWVNHPSYGTVWIP
                     SRSVVGAEFSPYVSAGHWALDDWGNWVWVSDYPFGRVVFHYGRWVMISGAGWAWVPGY
                     QYAPAWVSWRVPTGAYAYVGWAPLGPSFLWFNGFAVGYSYGYYTPWVFCSSAYVFHPN
                     VYRHVVRDRVLVDRLATSTRRYVPASPSVAGGPQRRYVSGPPPEVARVPARAVPRERL
                     RPAPGTAPPAERSRFFSSSPRRAEGIERSRVQPDVRRFDTPRGLTPGRPAPSERAYIP
                     RTRERAFEPGRSYTREPAAPRPRSFSPDRGGMPSRPSFSPPRSFSPSPSFSPPRSFSP
                     SHGFSPGGGGGRAPASPRRR"
     gene            1365..1919
                     /locus_tag="VFQ61_15570"
     CDS             1365..1919
                     /locus_tag="VFQ61_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955928.1"
                     /translation="MKPSNVLIGLSLLAGPVACSYGDRHYDDQHEIADPAACGAEPRQ
                     VGIDTDEALESDPGQGVGVFVEYTAGGRWHLFMTCDSNVSGQPCYFDVVAYSNNGAIS
                     NVTREDLESDDEISYKADYVQVVTTTDNDYDGVYLQTEPGDILSVDVYLDGSCYTEYL
                     WWVEGGEVRNGYIGTPLDFTPSEP"
     gene            2034..>2805
                     /locus_tag="VFQ61_15575"
     CDS             2034..>2805
                     /locus_tag="VFQ61_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955929.1"
                     /translation="MPFQDLAQHAAAVRVLSDFDLPRLGFSEFFEPAYGASQYLSVYA
                     LARLLTPAFGAVVAIKLVLASALVSTPYALRALLRALDKPAILALLCLPLTFNAHVVL
                     GFVHFVAGVPCMLFGLALGARSCRAPSIGTKLGLAAVGVLCFYTHVVPYAMLCVGTIL
                     LGASRSPARTVERSLPLGASALCALWWLRVSPSGATLRQLGRGWVSAARNEDRSVLRS
                     LEELPVWLSDVFGARFDDLSLAVWASLMIIVLLSGWSRV"
BASE COUNT          376 a          889 c          916 g          624 t
ORIGIN      
        1 ggcgagccag agcaaccctg ttcagggcgg tgctgttcag ggcggtgccg ttcagggcgg
       61 cggcctgagc ggtgcgccct cggcctcgaa tcaaggccca caggagatgc cgtcacttgc
      121 gccatcgccc ctgattacgg agcggcgtcc aacctcgagc gccttctcgg gccaggcgag
      181 cgccgagggc tccacgcagg tctcggccca agaccgtgat ccgcgcgcgt tgtccgattt
      241 tcgaccgtcg ctcgatccat acggctcctg ggtgaatcac ccgtcttacg gcacggtctg
      301 gattccctcg cgctccgtag tgggagctga gttttcgccc tatgtcagcg ctgggcactg
      361 ggccctggat gactggggca attgggtttg ggtaagcgat tacccgttcg gccgcgtagt
      421 cttccactat ggccgctggg tcatgatctc gggtgcgggc tgggcctggg tgcctggtta
      481 tcaatacgca ccggcttggg tctcttggcg cgtgcccacc ggcgcgtatg cctacgtggg
      541 ttgggctccc ctcgggccga gtttcttgtg gttcaacggt ttcgcggtgg gttactcgta
      601 cggctattac acgccgtggg tgttctgctc gagcgcctac gtattccacc ccaatgtcta
      661 ccggcacgtc gtgcgtgacc gcgtgttagt ggatcgcttg gcgacgagca cgcgccgtta
      721 tgttccggct tcgccttcgg tggcgggcgg gcctcagcgc cgttacgtga gtggcccgcc
      781 gcctgaagtg gcgcgtgttc ctgcgcgagc ggtgccgcgc gaaaggcttc gtccagctcc
      841 gggtacggcg cctcccgccg aacgcagccg gttcttctcg tcttcgcccc gacgcgccga
      901 agggatcgag cggtctcggg tgcaacctga cgttcgtcgg ttcgatactc cgcgcggcct
      961 aacccccggc cgccccgctc cctcggaacg agcctatatt ccgcgaacac gtgagcgagc
     1021 cttcgagccg ggtcgcagtt atactcggga gcctgcagcg ccccggccga ggtccttcag
     1081 ccctgaccgc ggtgggatgc cgtcccgtcc gagcttttca cctccccgaa gtttctctcc
     1141 ctctccgagt ttctcgcctc ctcgcagttt ttctccttcc catgggttca gcccgggcgg
     1201 cggcggcggg cgggccccgg catccccgcg tcgccgttga gcaggtcgcg gttcgacagc
     1261 gtcgcggttc gacagcgtcg cggttttcca acagagacga gtatcttgct tgaatgcccc
     1321 ctcggctgat cctgcggcca tgaactgaga ggttcctcga acacatgaag ccttcgaacg
     1381 tgcttattgg gttgtcgctg ctggccgggc cggtcgcgtg cagttacggc gatcggcatt
     1441 acgacgatca gcacgagatc gcggatccag cggcgtgcgg cgcagagcct cggcaggttg
     1501 ggatcgatac ggatgaagcg ctagaatccg atccgggcca gggggtgggc gtgttcgtgg
     1561 agtacaccgc gggcggccgc tggcatttgt tcatgacgtg cgattccaac gtctcggggc
     1621 agccttgtta cttcgatgtg gtggcctact cgaataacgg cgcaatctcg aacgtgacgc
     1681 gcgaagacct cgagagtgac gacgagatct cgtacaaggc cgactacgtc caggtcgtca
     1741 ccacgactga caacgactac gacggcgtct atctgcagac cgagcccggt gacatcctga
     1801 gcgtcgacgt ctacttggac ggaagctgtt acacagaata tctgtggtgg gtcgaaggcg
     1861 gcgaggtgcg gaacggctat atcggaacgc ccctggattt cactccgagc gagccgtaat
     1921 tctgcctgaa tccgattgga gctgcccgca tccgttggac agggtggagc ttcgaggcgt
     1981 ggtttgcggc gctctcgatt gcctccgccc tcccgctctt ttctgcgcat tatttgccct
     2041 tccaggactt ggcgcagcac gccgccgcgg ttcgagtctt atccgatttc gatctacccc
     2101 ggctcgggtt ctctgagttc ttcgagcccg cctatggggc ctctcagtat ctcagtgttt
     2161 acgctctagc gcggctgttg acgccggcgt tcggggccgt ggtggccatc aagctggtct
     2221 tggcgagcgc tctcgtgagc actccgtacg cgctccgggc tctgctgcgg gcgttggaca
     2281 agccagcaat cctggccctg ctctgcctac cgctgacttt caacgcgcac gtcgtactgg
     2341 gcttcgttca cttcgtggcg ggcgtcccgt gcatgctgtt tggcttggct ttgggcgcgc
     2401 gctcgtgtcg cgcgccgtcg atcgggacga agctcgggct ggctgccgtc ggagtgcttt
     2461 gcttttacac tcacgtcgtg ccgtatgcca tgctgtgcgt cggtacgatc ttgctgggcg
     2521 cgagtcgttc tcccgcccgc actgtcgagc gctcgttgcc gctcggtgct tccgcgctgt
     2581 gcgccttgtg gtggcttcgc gtgagcccct ctggggccac gcttcggcaa ctcggtcggg
     2641 gctgggtctc cgcggcgcgg aatgaagacc ggtccgtgct gcgcagcctc gaggaactgc
     2701 cagtttggtt gtcggatgtc tttggcgccc gcttcgacga cctctcgctt gcggtctggg
     2761 cgtcgttgat gatcatcgtg ctgctgagcg gatggagccg agtcg
//
LOCUS       DASTKU010000455        10127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1647449, whole genome shotgun sequence.
ACCESSION   DASTKU010000455 DASTKU010000000
VERSION     DASTKU010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10127
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647449"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="VFQ61_15580"
     CDS             complement(<1..1147)
                     /locus_tag="VFQ61_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9955930.1"
                     /translation="MNQDVISALQAALAASPANTKLLVGLLQAIATHTDGDQRNALER
                     EALAEAALGEVPGETDQQVIARALLRQQRAADALALLQGDAPESLVLRARVLVALDRT
                     EEASSAYRRAVEANATLQDVELERLLRAPVARSGGANQPRLRVISNDDTDQAEVVRLF
                     AAPAPRITFSDVGGLEEVKKQIRRKIVVPFQKPSIFARFKKKVGGGILMYGPPGCGKT
                     LLARATAGECNAEFFNVAISDILDMYIGESERKLHAIFEKVRAKTPAVLFFDELEALA
                     GKRQYSREGMSSKLVSQFLSEMDGFAQNNHGVLVLGATNVPWAVDPAFRRAGRFDRVL
                     FVPPPDMEARFHILSGMMRERPQHGVDLQAIAKRTSGYSGADLLNIVE"
     gene            1495..1827
                     /locus_tag="VFQ61_15585"
     CDS             1495..1827
                     /locus_tag="VFQ61_15585"
                     /inference="COORDINATES: protein motif:HMM:NF023740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3634 family protein"
                     /protein_id="HET9955931.1"
                     /translation="MNFWIGIVCILIFLVGVLWAARRANELFVLRIREGRVQHVRGRM
                     PPALYEAMREVAASPVIRNARVRGVVERGNPRLRVSGDVPPDQEQRLRNVLGMFPLPR
                     IRAGRRAP"
     gene            complement(1832..4207)
                     /locus_tag="VFQ61_15590"
     CDS             complement(1832..4207)
                     /locus_tag="VFQ61_15590"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9955932.1"
                     /translation="MTGFSEDLLLRQILALSSDALIGWNDGEPRGNADLRRKVVLWSS
                     AAERWFGYSEAEALALDVKQLFASDPDELEPNRPAAIDGIGRTGGRIGLDITTRRLPA
                     SAGSCRSLALLQLRASGSPSGELLGADAERLNLALAAARLGDWIWDASSDLVTFSPRA
                     AEIFGIPAGTHMTWTAMRELLHPEDRELARVAVERALASREDYSLEYRLINDERERWI
                     SASGRGRYDGSGKALGMFGVVQDITNDRLLVRLDDAVRPLVKSEDITYTSARLLGQHL
                     NVDRCAYAFVEEDQDTFLLTGNYTRGVPSIVGRYRFRQFGAECLRLMRAGEPYIVRDS
                     SNDPRIDAEDRKAYAATQIVAVICVPIVKSGKFVAAMAVHCRTPRRFRPSEVALVQQV
                     ASRSWESIERTRVERERESLLESAESANRAKDEFLAMLGHELRNPLAPIQTALQVMKL
                     RDDADSARERALIERQVNHLTRLVDDLLDVSRIARAKVVLKFETLELADVVRQAIELV
                     SPLIEQRAHQLVVAVAGSGLLIEGDAARLTQVVSSLLTNAAKYTPSGGQIRISGSLEE
                     DYVVLRIRDNGMGIAPDVLPRIFDLFVQGQQAIDRALGGLGLGLTIVHSLVERHGGRV
                     TASSDGPGRGSEFVVQLPRAKESSSRIRGPEARASFKPRSGVGLRVLVVDDNEDAAEM
                     LGHALALRGYEVHLAHDGPHALRLAAAQAFDAALLDIGLPIMDGYELAARLLALPSLA
                     HAKLIAVTGYGQDSDRRRALAAGFHHHLVKPVELGVLDALLTGSATPNRTP"
     gene            4568..4960
                     /gene="raiA"
                     /locus_tag="VFQ61_15595"
     CDS             4568..4960
                     /gene="raiA"
                     /locus_tag="VFQ61_15595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET9955933.1"
                     /translation="MHLYITARHFDLTPSIREYVEKRLVNAIEGHATAHDLVRMEVQL
                     TELDGHAVRFRCHILLQLPNNHDINITEETLDLYEAVDRAEKRLIRKLTDERQRYISA
                     QRHGKQQEVAPSIEPEEVPEASTTESAG"
     gene            5184..6425
                     /locus_tag="VFQ61_15600"
     CDS             5184..6425
                     /locus_tag="VFQ61_15600"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HET9955934.1"
                     /translation="MTRCWARFVARLTVPGLLTLSCAAVSSCLLSSCANTPSAVPLQA
                     PPPPVSDVSPTPEPTPPAATKAPTIVSASFGQIDNQAVELYTLSNRNGLVMKVSTFGA
                     AITEFHVPDRHGKLADIVLGFDTLDGYLNDTTFMGAIVGRLANRLRNAKFTLEGREFK
                     LATNSGAHHMHGGVRGFNKVVWSAEPLESAQGPSLKLTYVSKDGEEGYPGTVTAKVTY
                     TLTHENEFRVEMEATTDKVTLVNLAQHTYWNLGGHDSGTILDHELTLYADRYTPGDPV
                     VPTGAVRSVKSTPFDFSAPKPIGKDLKAAGGDPVGYDHNFLVNGDPNALRPVARLRDP
                     KSGRVLFLESDQPAVQFYSGNFLNGSSQGKGTKYIQYAGLCLETQKVPNAVNLPAWRE
                     DVILKPGQTYRHTMLFHLSNE"
     gene            complement(6453..7196)
                     /locus_tag="VFQ61_15605"
     CDS             complement(6453..7196)
                     /locus_tag="VFQ61_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955935.1"
                     /translation="MSDVTFAAVFSDVSSANKALDRLEQERGTSRGLSLLMRERTAER
                     ELAVGEGAADVDTPLPLGPGALRLASELQPVVSLAPGINWLVAGPLGSWLERVALGSA
                     LELSEALCRSGVDAGTAETAVNALRTGGMLVALELNEAAIGDARSEYENFREATVGCL
                     SGFELTFDTALPAAAPTSTSRESTRAPDAESGSELQPSEAVIPLEAALQGPRPEQVGA
                     IPPGEGDADFSSDTEPSQAERNLQSSSHE"
     gene            complement(7315..8559)
                     /locus_tag="VFQ61_15610"
     CDS             complement(7315..8559)
                     /locus_tag="VFQ61_15610"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955936.1"
                     /translation="MNGTRVHQILLVPGFFGFGRFGEISYFNGVADVLTRALARQGIA
                     AQVNEVATVPTASIRVRAARVLEALERLQSTTDGPIHIIGHSTGGLDARLAIAPTASL
                     PTDRHFTAPERLRTLVTVCCPHFGTPIATFFCGGWGRALLRVICSYLVWLLLTGRLPL
                     RVFLKLGYWIVRVRDPFRKRRTTFDEVYEKLLNDLSEPRRQELAAFLKAVAEDQSLVF
                     QLTPAGCDLLNACTAEPNVHYASVVARAERPSWRYWLSSVWDLYTQMVHPFYGWLHRL
                     STRAEVRFIPAPVTKQAQVLEQAYGRTLALTDSDGLVPTLSQVWGDVIHAARADHLDV
                     VGQFGRIESDSWAGDWLPSHSGFDRSQFDALWSDVARYIATHSERELPPNGVGTDRTD
                     RDLPDAVSEAARSELQPAGQKR"
     gene            complement(8556..9344)
                     /locus_tag="VFQ61_15615"
     CDS             complement(8556..9344)
                     /locus_tag="VFQ61_15615"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9955937.1"
                     /translation="MKSKPSKRLLTELYELTHRGHPGDEAFYVNACRGARHVLELGCG
                     TGRLLVPLAKVARRVTGLELEPSMLQAAERKLEFQTASVRRRVDLCRGDMARIALGKR
                     FDRIVLPYNALYCLLSRRGVRDCFRGVREHLADGGEFLFDVWLADAFHAAAAGNDYRD
                     DEEAITRIEHRGRVWEVYERSRLRRARRRLDVHYLYVPSTGGAIVDCLIQQRYAPSAE
                     LRSDLAAAGLRVDAQWGEFDLRRFTRRATRWIVRAKAVASDEGR"
     gene            complement(9341..>10127)
                     /locus_tag="VFQ61_15620"
     CDS             complement(9341..>10127)
                     /locus_tag="VFQ61_15620"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9955938.1"
                     /translation="VAQLDQTVGPLSVNHAGQLPAATVSFNVRPGHSLSEAVEAANAI
                     ARRTLPSGINASFQGSAEAFQSSLKGLGMLLVISVFVIYLVLGILYESTLHPLTILSA
                     LPFAGLGALGTLYVFGMDLNVYAFVGVILLVGLVKKNGIMMVDFAIEARKAADTSAEA
                     AIREACLVRFRPIMMTTLAALFGTLPIALGIGAGAESRQPLGLSVVGGLFFSQILTLY
                     VTPVIYLYMERLREWGERKLAARREQRARSRAGHAGWAEKTAE"
BASE COUNT         1970 a         3321 c         3043 g         1793 t
ORIGIN      
        1 tctccacgat gttcaacaag tccgccccgg agtagccgga ggtccgcttg gcaatagctt
       61 gcaagtccac accatgttgc gggcgttccc gcatcatgcc gctcaaaatg tgaaatcggg
      121 cttccatatc cgggggcggc acgaacagca cgcgatcgaa gcgccctgcg cggcggaagg
      181 ctggatccac cgcccacggc acgttggttg cgcccagcac cagcacgccg tgattgttct
      241 gagcgaaccc gtccatctcc gagagaaact ggctcactag cttggaagac atgccttctc
      301 gcgagtactg acgtttcccg gccaaagcct ccagctcatc gaaaaacagc accgccggcg
      361 tcttggcgcg aaccttctcg aagatggcgt gcagcttgcg ctcgctctct ccgatataca
      421 tgtccaagat gtccgagatc gccacgttga agaattccgc gttgcactcg ccggccgtgg
      481 cccgcgcgag caacgtcttg ccgcaaccgg gcggaccgta catcaggatg ccgccgccca
      541 ccttcttctt gaagcgagca aagatcgacg gcttctgaaa tggcaccacg atcttgcgac
      601 ggatctgctt cttgacctct tcgagccccc ccacgtccga gaacgtgatg cgcggggccg
      661 gagccgcgaa cagccgcacc acctccgctt gatccgtgtc gtcattggag atgacccgca
      721 gccgcggttg attggctccc ccggagcgag ccacgggagc gcgcaacaaa cgctcgagct
      781 ccacgtcctg cagggtggcg ttcgcttcca cggctcgtcg atacgcgctc gaggcctctt
      841 cggtgcgatc gagcgccacc agcacgcgcg cccgcaacac gaggctctcg ggggcgtccc
      901 cttgcaagag cgccaaggcg tccgccgcgc gttgctgacg cagtagcgca cgcgcgatca
      961 cctgctggtc tgtctcgcct ggcacctcgc ccaacgcagc ctcggcgagc gcctcccgct
     1021 cgagcgcgtt acgctgatcc ccgtccgtat gcgtcgcgat ggcttgcagc aaacccacga
     1081 gcagcttggt gttggcgggg ctcgcggcga gcgccgcttg cagggccgaa atcacatcct
     1141 gattcatcga gagaactcgt cgcgaaaggc cgaacacgcg caacccggcc ggaaacccga
     1201 ggcatatcat cgcgccttcg ctgcgccaac cacctgagaa tggcgggccg cgctgtaacg
     1261 ggcgcgcggc tcggcgggtc agcggctcga gcgcagcccc ttcacggccg tccccggcca
     1321 attaccgcgc tggcccccgc aatagtcgcc ccgcagtcgc aaggtatgcg gcagcttcgc
     1381 ggcggcgccc gatcccagac gggcgtcgat agagacttcc ccagctaggc tgccggagat
     1441 agacaaggag cgccaaatcc aagtcgaaga cgctctcccc ttcagctcag gctcatgaat
     1501 ttctggatcg gcatcgtttg catcctcatt ttcctcgtgg gcgtcctatg ggcggctcgg
     1561 cgcgccaacg agctcttcgt gctgcgaatt cgcgagggtc gagtgcagca cgtgcgcggg
     1621 cgcatgccgc cggcgctcta cgaggccatg cgagaggtgg cggcgagccc agtgattcgc
     1681 aatgctcggg tccggggtgt cgtcgagcgg ggtaaccctc ggctacgagt cagcggtgat
     1741 gttccgcccg atcaagagca gcgcctgagg aacgtgctcg ggatgtttcc tttgccaagg
     1801 atccgtgctg gccggcgcgc cccgtagccg gtcacggagt gcggttcggc gtcgcgctgc
     1861 cggtgagcag tgcatcgagc acgccgagtt ccacgggctt gacgagatgg tgatgaaagc
     1921 cagcggccaa ggcgcgtcga cgatccgagt cttgcccgta acccgtcacc gcgatgagct
     1981 tggcatgcgc gagggacggt aaggccagca gtcgcgccgc cagctcgtag ccatccatga
     2041 tcggcagtcc gatatcgagc agcgcggcgt cgaaagcttg cgcggcagcg agccgcaaag
     2101 cgtgggggcc gtcgtgcgcg agatgtactt cgtatccgcg caacgcgagc gcatgaccca
     2161 acatctcggc cgcatcctcg ttgtcgtcga ccaccagcac tcgtaggccc acccccgagc
     2221 gaggtttgaa ggatgcccgg gcttctggtc cgcgaatgcg cgagctgctc tccttggccc
     2281 gcggtagctg cacgacgaat tcgctgccac gcccgggccc gtcgctactg gcggttacgc
     2341 gcccaccatg gcgctcgacg aggctgtgaa cgatggtgag cccgagcccc aggccaccca
     2401 acgcacgatc gatggcctgc tggccctgga cgaacagatc gaaaattcga ggcagcacgt
     2461 ccggcgcgat gcccatcccg ttgtcccgaa tccggagcac gacgtaatcc tcttcgaggc
     2521 tgcccgagat gcggatctgg cctcccgagg gcgtgtattt ggcggcgttc gtcagcaagc
     2581 tactcaccac ttgggtgagc cgagccgcgt ccccttcgat gaggagcccc gagcccgcta
     2641 cggctacgac cagttggtgg gctcgctgct cgatcagcgg gctgacgagc tcgatggcct
     2701 gacgaaccac gtccgcgagc tctagtgtct cgaacttcag cacgaccttg gcgcgcgcga
     2761 tgcgtgaaac gtccagcaga tcgtccacca agcgagtcag atggttgacc tggcgctcga
     2821 tcaaggcgcg ttcacgcgca gagtcggcat cgtcacgtag cttcatcacc tgaagcgccg
     2881 tttgaatggg tgcgagcggg tttcggagct cgtggccgag cattgccaaa aattcgtcct
     2941 tggctcgatt ggcagactcg gcgctctcca gcagagactc gcgctcccgt tcgactctcg
     3001 tgcgctcgat cgattcccaa gagcggctcg cgacttgttg caccaacgcg acttcggacg
     3061 gccgaaagcg ccgcggggtt cggcagtgca cggccatggc ggcgacgaac ttgccggact
     3121 tgacgatcgg cacgcaaatc acggccacga tctgggtcgc cgcgtaggcc ttccggtcct
     3181 cggcgtcgat gcgcggatca ttcgagctgt ctcgcacgat gtagggctcg ccggcacgca
     3241 tcaagcgcag acactcggcg ccgaactgcc gaaaccgata acggcccacg atactcggga
     3301 cgccccgcgt gtagttcccc gtgagtaaga acgtgtcctg gtcctcctcc acgaacgcgt
     3361 acgcgcaacg atccacgttc aagtgctggc cgagcaggcg cgccgacgta tacgtaatgt
     3421 cctctgactt gaccaacggg cgcaccgcat cgtccaggcg caccaagagc cgatcgttgg
     3481 tgatgtcctg caccacgccg aacattccga gcgcctttcc ggaaccatcg tagcggccgc
     3541 ggccgctcgc gctgatccag cgctcccgtt cgtcgttgat caaacggtat tccagcgagt
     3601 agtcctcacg cgaggcgaga gcgcgttcga ctgccacccg cgccaactcc cgatcttccg
     3661 ggtgcaacag ctcccgcatg gccgtccagg tcatgtgggt tcccgctgga ataccgaaaa
     3721 tctccgccgc gcgcggcgag aacgtcacta agtcggagct cgcgtcccag atccagtcgc
     3781 ccaggcgcgc cgcagccaag gcgaggttca atcgctccgc gtctgcgccg agcaactcgc
     3841 ccgatgggct tccggaagca cggagctgga gcaaagccaa gctccgacat gatccggcgg
     3901 aagccggcag ccgacgcgtc gtaatatcca ggccaatgcg ccctccggtg cggccaatgc
     3961 cgtcgatagc ggcagggcga ttcggttcta gctcatccgg atcggacgcg aagagctgct
     4021 tcacgtccaa agcgagagcc tcggcctccg agtatccgaa ccacctctcc gcggctgagc
     4081 tccaaagcac cactttgcgc cgaagatcag cgttccctcg cggctcgccg tcgttccacc
     4141 cgatcagggc atcgctcgag agcgctagga tttgacgcag caggaggtct tcggagaaac
     4201 ccgtcaagct cttgctgtta tcacagatcg gaaagggagc gcgcggcgcg agcgatccgt
     4261 tgcttgtgca ggctagccct ttctataacc agcgccgcga ccctcttcga tcggtcctct
     4321 cccgactctt gcgcagcttt tgccgctgct actctcggcg ccgcggaatt gccgtccctc
     4381 ggctgcttgg gtcacggccc gaccagctgg gtgaaaggac tcagcgcacg ctgccacgcg
     4441 cgatctgtag ccatttccgc cacaagaatc gtaatccgtg caccgggcca atgttgcact
     4501 cgtgaattcc ctcgggactg acggtttcgg catggtgact gcatggtgag agcacggagc
     4561 atttgccatg catctgtaca ttacggctcg tcatttcgat ttgactccca gcattcgaga
     4621 gtacgtcgag aagcgcctgg tcaacgccat cgaaggccac gcgaccgcac atgacttggt
     4681 ccgaatggag gttcagctca cggagctgga cggacacgca gttcgctttc gctgtcacat
     4741 attgctgcaa ctgccgaaca accacgacat caacatcacc gaggagacgc tcgatcttta
     4801 cgaggccgtc gatcgcgctg aaaagcgact catccgcaag ctgacggacg aaaggcagcg
     4861 ctacatttcc gcgcagcgcc acggcaagca acaggaagtc gcaccgtcca tcgagcccga
     4921 agaggtgccc gaggcgagca ctacggagag cgccggttag acgcgccccg tagaatatcg
     4981 gcacagagga tccgcgcgca gtttgggcca ccgctcagcc cgagccatcc cgattcatgg
     5041 gtatgccatc gatactgcgc gcggacttct gtgaaccgcc tccgctgact tgagaaaact
     5101 tggcacccgg gagtatgttc gcctcgtgac tgcagcggag cattcatatt ttcgagcttt
     5161 ggctctggta acggaggaac ggcatgactc gatgttgggc tcggttcgtt gcccgtctca
     5221 cggtccctgg attattgact ctttcgtgtg cggcagtgtc gagctgtttg ctctcgagtt
     5281 gtgccaacac tccttccgcc gttccgctgc aagcgcctcc ccctccggtc tctgacgttt
     5341 ccccaacccc cgaacctaca ccccccgccg caacgaaggc cccgacgatc gttagcgcga
     5401 gctttgggca gatcgataac caagcggtcg aactctacac cctgagcaat agaaacggcc
     5461 tcgtaatgaa ggtgtcgact ttcggcgccg caattaccga gtttcacgta cctgaccggc
     5521 acggcaagct agccgatatc gtgctggggt tcgatacgct cgatggctac ctgaatgaca
     5581 cgacgttcat gggcgccatc gtgggtcgtc tcgcgaaccg acttcggaat gcgaagttca
     5641 cactcgaggg tagggagttc aaactcgcca ccaacagcgg cgcacaccac atgcacggtg
     5701 gggttcgcgg cttcaacaag gtggtgtgga gtgcagagcc gctcgagtct gcccaaggtc
     5761 cctcgctcaa actgacgtac gtctcgaagg atggtgagga gggctacccg ggcaccgtga
     5821 ccgcgaaagt aacgtacact ctgacccacg agaacgagtt ccgagtggaa atggaggcca
     5881 cgaccgacaa ggtcacgctc gtgaacttgg cccaacacac gtattggaac ttgggcgggc
     5941 acgactcggg caccattctg gatcacgaat tgaccctata tgccgatcgg tatacacccg
     6001 gagatccggt agtaccgacg ggtgcggttc gctccgtcaa gagtacgccc ttcgatttct
     6061 cggcgcccaa gcccattggc aaggatctga aggccgcggg cggagatccc gtcgggtacg
     6121 accacaactt cttggtcaat ggtgatccca acgcgctacg gccggtggcg cgcctgcgag
     6181 accctaagtc tggtcgggtc ttgttcctgg aatcggatca gccggcggtg cagttctact
     6241 ccggcaattt tctgaatgga tcgagccaag gcaagggcac caagtacatc cagtacgccg
     6301 ggttgtgttt ggagactcag aaagttccga acgccgtgaa tctccctgcg tggcgtgagg
     6361 acgtgattct caagcctggg caaacttacc gtcacacgat gctatttcat ctcagcaacg
     6421 agtaacactc tgggcttatt tcaccgaacg tcttactcgt gcgaagagct ttgcaggttc
     6481 cgctcggctt ggcttggctc ggtgtcgctc gagaaatcgg cgtcaccctc gccgggagga
     6541 atagcaccaa cttgctctgg ccggggtcct tggagcgcag cttccagtgg aatcacagcc
     6601 tcgctgggct gcagttcgct ccccgattct gcgtcagggg cgcgagtaga ctcgcggctc
     6661 gtcgaggttg gagctgccgc gggcaaggcg gtgtcgaacg tgagttcaaa ccccgacagg
     6721 caccccacgg tggcctcacg aaagttctcg tactcacttc gtgcatcccc gatcgcggcc
     6781 tcgttgagct cgagagcaac gagcatcccg cccgttctga gcgcgttcac ggccgtctca
     6841 gcggtgccag cgtctactcc cgaacggcac aaagcctcgg acagctccag cgccgatccg
     6901 agcgctaccc gctcgagcca cgaaccgaga ggccccgcca ccaaccagtt gatccccggt
     6961 gccagcgaca ccaccggttg cagctcggac gcgagacgca gcgccccagg ccccagaggc
     7021 agcggagtat ctacatcagc tgccccctca cctaccgcga gttcgcgctc ggcggtacgt
     7081 tcgcgcatca acaagctgag ccctcgtgag gtgccgcgct cctgctccaa tcgatccaac
     7141 gccttgttgg ccgagctcac gtccgagaac accgcggcaa atgttacgtc cgacatgccc
     7201 gaactcgagc aaaccgcgta ccaataggac tagagcgcca gaagtgaact cagcggttct
     7261 catgccctcg gctcactcgg aatggggaat tctggcgcgc agcgatgccc cagactagcg
     7321 cttctgaccg gcaggttgga gctcactccg tgcggcctcg ctcaccgcat ccggtagatc
     7381 tcgatcggtt cgatcggtgc ccacgccgtt cgggggaagt tcgcgctcgg agtgggtcgc
     7441 gatataacgg gccacgtccg accacaaagc atcgaattgg gaacggtcga agccggaatg
     7501 gcttggaagc cagtctcccg cccatgaatc ggattcgatc cgcccgaatt gccccaccac
     7561 gtccaagtga tcggcacgcg cggcgtggat cacgtctccc caaacttggg aaagcgtggg
     7621 gacgagccca tcgctgtcgg tgagcgcgag agtccgcccg taagcttgct ccaaaacctg
     7681 tgcctgtttg gtgactgggg ccgggatgaa gcggacctcc gcgcgcgtgg agagccggtg
     7741 gagccaaccg tagaaggggt gaaccatctg ggtatagagg tcccaaacgc tgctcagcca
     7801 atagcgccag gaagggcgtt cggccctcgc gaccacggat gcatagtgca cgttgggttc
     7861 cgcggtgcag gcattcaaca agtcgcagcc cgccggagtc aactgaaaga ccagcgattg
     7921 gtcctccgcg acggccttca aaaaggccgc gagttcctgg cggcgcggct cgctcaagtc
     7981 gttcagcagc ttctcgtaaa cctcgtcgaa agtggtgcgt cgcttccgaa acggatcacg
     8041 cacgcgcacg atccaataac cgagcttgag gaaaactcgc agtggtaggc ggccggtgag
     8101 cagcaaccac acgagatagg agcaaattac gcgcagcagc gcgcgccccc agcctccgca
     8161 aaagaaggtg gcaatgggcg tcccaaagtg agggcaacag accgtcacca gcgtgcggag
     8221 ccgctccggc gcagtgaaat gccgatcggt cggcaaagac gcggtaggag ctatggcgag
     8281 acgcgcgtcc agccctccgg tggagtgacc gatgatgtgg atggggccat cggtggtgct
     8341 ctgcaagcgc tccaacgcct cgagaacccg agccgcccgc actcggatcg atgccgtggg
     8401 cacggtcgcg acctcgttca cctgggccgc gatcccctgc cgggccaggg ctcgagtcag
     8461 tacgtctgca accccgttga agtacgagat ctcaccgaat ctaccgaaac cgaagaaccc
     8521 cggtacgagc aaaatctgat ggacccgagt cccgttcacc ggccctcgtc cgaagcgacg
     8581 gctttggcgc gcacgatcca gcgcgtcgct cggcgcgtga accgacgcag atcgaactca
     8641 ccccactgcg cgtccacgcg caatccggca gcggccagat cactccgaag ctcggcagat
     8701 ggcgcgtagc gctgctgaat caagcaatcg acgatggctc cgcccgtact cggcacgtac
     8761 aaatagtgaa cgtctaatcg ccgcctggct cggcgcaatc gactgcgctc gtaaacttcc
     8821 cacactcgcc cgcggtgctc gatacgagta atcgcctcct cgtcatctcg atagtcgttt
     8881 cctgcggcgg cggcgtggaa cgcatcggct agccacacgt cgaacaggaa ctctcccccg
     8941 tcggcgagat gctctcgtac tcctctgaag cagtcgcgaa ccccgcggcg gctcaacaag
     9001 cagtacagag cgttgtaggg cagcacgatg cgatcgaagc gttttccgag tgcgattcgc
     9061 gccatgtccc cacgacacaa atccactcgc cgacgcactg aagccgtctg aaactccagc
     9121 tttcgttcgg ctgcctgcaa catggacggc tcgagctcca atcccgtcac gcgccgcgcg
     9181 accttggcta aaggcaccag cagccgcccc gttccgcagc cgagttcgag cacatgacgc
     9241 gccccgcggc aggcattcac gtaaaacgcc tcgtcgccag gatgcccgcg atgggtcagc
     9301 tcgtaaagct cggtcagcag acgtttcgaa ggctttgatt tcactcggcc gtcttctccg
     9361 cccagcccgc gtgcccggct cgacttcgcg cgcgctgctc ccgccgcgcc gcaagcttac
     9421 gctcgcccca ctcgcgcagg cgctccatgt agagatagat aacgggggtc acgtacaagg
     9481 tcaggatttg ggaaaagaac aatcctccta ccacactcag cccgagcggc tgcctagact
     9541 cggcgcctgc gccaatcccg agcgcgatgg gcagcgtacc gaagagggcc gcgagggtgg
     9601 tcatcatgat gggtctaaaa cgcaccaagc aagcctctcg aatcgcggct tcggcggacg
     9661 tgtcggcagc ttttcgggct tcgattgcga agtcgaccat catgatgccg ttctttttga
     9721 ccaaacccac gagcaggatc acgccgacga aggcgtagac gttcaagtcc atgccgaaga
     9781 cgtagagcgt gccgagcgca cccagtccgg cgaatggcag cgccgagagg atcgtcaaag
     9841 ggtgcagcgt gctctcgtaa aggattccaa gcaccaggta gatgacgaac accgaaatca
     9901 cgagcagcat cccgaggcct ttcagagacg actgaaatgc ctcagccgaa ccttggaagc
     9961 tggcgttgat tccggaagga agagtgcggc gggcgattgc gttggccgcc tccacggctt
    10021 cgctgagcga gtggcccggc cgtacgttga acgataccgt ggcggccggg agctggccgg
    10081 cgtgattcac ggacagcggc cccacggttt gatctagctg cgcaacc
//
LOCUS       DASTKU010000456         4020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1871081, whole genome shotgun sequence.
ACCESSION   DASTKU010000456 DASTKU010000000
VERSION     DASTKU010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871081"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..589
                     /gene="rnpA"
                     /locus_tag="VFQ61_15625"
     CDS             92..589
                     /gene="rnpA"
                     /locus_tag="VFQ61_15625"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET9955939.1"
                     /translation="MSERGASKTDRGKHGFSKDSRVRKRREYQDIQARGRRVSSAHFV
                     LILVMRDTSARASHVCPHPGVTRLGITASRKVGSAVVRNRMKRLVRHAFVEVRDWFPN
                     ELDVVVVVRSAWPEAKVEDVISEWRSVDRNLRKQIQEARKLSVNARASSTQTGGSSRN
                     DKTGP"
     gene            666..1001
                     /gene="yidD"
                     /locus_tag="VFQ61_15630"
     CDS             666..1001
                     /gene="yidD"
                     /locus_tag="VFQ61_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET9955940.1"
                     /translation="MSSQLSSPSRATEEQREQELLDAALSQARTGLVTRALLGAIRLY
                     QLLLSPYFGQVCRFHPSCSRYTAECLCLHGPLKGSYLGMRRLLRCHPFHPGGVDLPPR
                     PVARAPQGH"
     gene            1167..3023
                     /gene="yidC"
                     /locus_tag="VFQ61_15635"
     CDS             1167..3023
                     /gene="yidC"
                     /locus_tag="VFQ61_15635"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET9955941.1"
                     /translation="MDRGNIGRFLLLAAAVLLLMRFAPSLFGNSSTPERQPLHPEGDR
                     SLPAEQRPAEKFCELWGSGFHAQLSTRGASIRKFELTSAKYQKDGKPTQLSTTPEVEA
                     RRQLRFQFENPAARVAEADRQLRYDLVDYEIRSSDGKQCEFVYRDDTVELVQTVKTTS
                     RPYELVAEAKLTNLAATPRTHALTVHTDAWRMESEVHGGMFRVSPLLTHVECVASDGT
                     TKRLTPQDFEPADFEKVENFPRTATSFGDWFQAPGEPALAAVSNAYFSHALVPLSGPR
                     PVCQMQIEDRWDARQYQNKGADPHGGAMYRARLAYPAKKLAPGESSVYSVLTYVGPKE
                     RSVLSGAAGGNHKLIELIDLGFFSAIAKVLVAFLLKVHSVIPNWGIAIIVLTITARTL
                     LFPLALPGIKGMIKMRELKPEIDALNEKYKDDPQAKGLAQMQLWRKHNVNPVKGCLPQ
                     MASMPVWFALYTTLQTAVELYNIPFLWFPDLSAADPLYILPFIIGATSFMQQRLMPIQ
                     ADPAQQKMMLYFMPAMFTVFMLFLPAGLGVYMFTNGLLGIIQQQAVERYVRRNVRPGG
                     GSGSGDIQVKVLEDSGKRTGEKQGKGSAKQGHPPSGASSSKEDSRLLGEGKA"
     gene            3027..3728
                     /locus_tag="VFQ61_15640"
     CDS             3027..3728
                     /locus_tag="VFQ61_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HET9955942.1"
                     /translation="MSDTQDFSNEGWEDGDDDDNDVDSDDELGSADDGLDEKAGSPSA
                     AGPAADSADGSRRRRSRRGRDREDPGQFDERARRAQAFVEEVVRKMGMDCRVRLRRPR
                     EDDAADEINLEILGRDAGRIIGKKGQVLQALQFLVHRVVNRPGLERRHILVDAEGYRS
                     RRDNSLASMARRLGKQAVDEGKIITFEPMNPRDRRVVHLALAKFEGVITKSDGEGDQR
                     RVQIIPVRRPSPEPS"
BASE COUNT          759 a         1250 c         1269 g          742 t
ORIGIN      
        1 cctcgccaat cgtcgccgta aggggcgctg gcgtctgacc gtgtctgcct accagaagta
       61 gtgctgatcc caacgagtag ttgcggtccg cttgtccgag cgcggcgctt cgaaaacaga
      121 ccgtggcaag cacggttttt cgaaggatag ccgcgtccgc aagcgccgcg agtaccaaga
      181 tattcaggcc cgtggtcggc gtgtctcgtc agcacacttc gtgctgatcc tcgtgatgcg
      241 agacacgtct gctcgggcct cgcatgtctg cccgcaccct ggagtgacgc gcctggggat
      301 aacggcatcc cgaaaagtcg ggagcgcggt cgttcgtaac cgcatgaagc gtttggtgcg
      361 ccacgccttc gtcgaagtgc gtgattggtt tccaaacgaa ttggatgtgg tcgtcgtcgt
      421 tcgaagcgcc tggcccgaag ccaaggtgga ggacgtcata tccgagtggc gctccgtgga
      481 ccgcaacctc cggaagcaaa ttcaggaggc gcgcaagctc tccgtgaacg cgcgggcgtc
      541 ctcgacccaa acgggtggct cgagtcggaa cgataaaaca ggtccgtagc gaggactagg
      601 ccttcacaaa gaaggcctcg cttcgagcga tggtgctcga gttgggtcaa actcgtcggc
      661 ggcgaatgtc gagccagctt tcgagtccga gccgagccac cgaagagcag cgggagcagg
      721 agctcctcga cgcggctctt tcgcaggcgc gaactggcct ggtaacgcgc gctctgctcg
      781 gcgcaattcg gctgtatcag ctgctgctct caccgtattt cgggcaggtc tgccgctttc
      841 atccgtcgtg ctcgcgttac accgccgagt gcctgtgcct gcatggcccg ctcaagggtt
      901 cttatctagg catgcgacgg ctactgcgtt gtcacccatt ccaccccggc ggtgtcgatc
      961 tacctccacg accggttgcg cgggcccccc agggtcacta ggtctcccca aagacgacca
     1021 gcccgagcag gtgcccagct agttccctcc ccgaaggtca cgaggaaaaa ccggttgagc
     1081 ccacgttggc ggcgaaagcc aatcgaagtc gcgactgaaa ggtgctaggc gaaggctctt
     1141 cttctctccc ggcacgacga aaatccatgg atcgaggcaa tatcgggcgt ttcctgctgc
     1201 ttgctgcggc tgtgctgctg ttgatgcgct ttgcgccttc gctgttcggc aacagcagca
     1261 ctcccgagcg ccagccactt cacccggagg gcgatcgctc gctccccgct gagcaacgcc
     1321 cggcagagaa attctgcgag ctgtggggct cgggctttca cgcccagcta tccacgcgcg
     1381 gcgccagcat tcgcaagttc gagctgacct cggccaagta tcaaaaggat ggaaagccaa
     1441 cccagctctc caccaccccc gaggtcgagg cccgacgtca gttacgcttt cagttcgaga
     1501 atcccgcggc gcgcgtggcg gaggcggatc gccagctgcg ttacgatttg gtcgactacg
     1561 agattcgttc gagcgacggc aagcagtgcg agttcgtgta tcgcgacgac acggtcgagc
     1621 tggtgcaaac cgtcaagact acgtcccgtc cctacgagct ggtggcggaa gccaagctca
     1681 cgaacctggc cgcgactccg cgcactcacg cgctgacggt gcacaccgat gcctggcgca
     1741 tggagtcgga ggttcacggc ggcatgttcc gggtgagccc gctgctcacg cacgtggagt
     1801 gcgtcgcgag cgatggcacc accaagcggc tgacgccgca ggatttcgag cctgcggact
     1861 tcgagaaggt cgagaatttc ccgcgcacgg caacgagctt cggcgattgg ttccaggcgc
     1921 cgggcgagcc cgctttggcg gcagtctcca acgcctactt ctcgcacgcg ctggtaccgc
     1981 tgagcggccc gcgcccggtc tgccagatgc agatcgagga tcgctgggac gcgcgccagt
     2041 atcaaaacaa gggagccgat ccacacggcg gggcgatgta ccgcgcgcgg ctggcttacc
     2101 ctgcaaagaa gctcgcgccg ggtgagtcga gcgtttactc ggtgctcacc tacgttggcc
     2161 ccaaagagcg cagcgtgttg tcgggcgccg ccggtggcaa tcacaagctg atcgagctga
     2221 tcgatctcgg cttcttctcc gcgatcgcga aggttctggt ggccttcttg ttgaaggtgc
     2281 acagcgtcat cccgaactgg ggaatcgcga tcatcgtgct gacgatcacc gcgcgcacgt
     2341 tgctgttccc gctcgcgctg cccggcatca agggcatgat caagatgcgc gagctgaagc
     2401 cggagatcga cgcgctcaac gagaagtaca aggacgaccc gcaggccaag ggtcttgccc
     2461 agatgcaact ctggcgcaaa cacaacgtga acccggtgaa gggctgcttg ccccagatgg
     2521 cgtcgatgcc ggtctggttc gccctctaca ccacgttgca gacggccgtg gagctgtaca
     2581 atattccgtt cctgtggttc cccgacctct ccgcggcgga ccccctgtac attctcccct
     2641 tcatcatcgg cgcgacatcc ttcatgcagc agcggttgat gccgatccag gcagatccgg
     2701 cccagcagaa gatgatgctc tatttcatgc cggcgatgtt caccgttttc atgctgtttt
     2761 tgccggctgg cctcggggta tacatgttca ccaacggttt gctcggcatc attcagcaac
     2821 aagcagtcga gcgttacgtt cgccgcaacg tccggcccgg tggcgggtcc ggttctggcg
     2881 acattcaagt caaagtgctt gaggattccg gcaagcgcac gggcgaaaag cagggcaaag
     2941 ggtcagccaa gcagggccac ccgccgtccg gggccagctc gagcaaggaa gacagtcggc
     3001 tcctcgggga aggtaaggcg taaaagatga gcgacaccca ggacttctcg aacgagggtt
     3061 gggaagacgg cgacgacgac gataacgacg tcgactccga cgacgaactc gggagcgccg
     3121 acgacggcct cgacgaaaag gccggcagcc ccagcgctgc gggccccgcc gcggattcgg
     3181 ccgacggaag ccggaggcgg cgtagccgcc gcggccgtga ccgcgaagat cccggccaat
     3241 tcgacgaacg ggcgcggcgc gcgcaggcat tcgtggaaga agtcgtccgc aagatgggta
     3301 tggattgtcg ggtgcgtctc cgacggccca gagaggacga cgcagcagac gagatcaatc
     3361 tggagatcct gggccgcgat gccggccgaa tcattgggaa aaagggtcaa gtcctgcagg
     3421 cgctgcagtt cctggttcat cgcgtggtga atcgccccgg tctagagcgg cgtcacattc
     3481 tggtcgacgc cgaggggtat cgcagtcgcc gagacaactc gctggccagc atggctcgcc
     3541 ggttgggtaa acaggccgtg gacgaaggca agatcatcac gttcgagcct atgaatccgc
     3601 gggaccgtcg cgtagtgcac ctcgcgctcg cgaagttcga aggcgtgatc acgaagagtg
     3661 acggcgaggg cgatcaacgc cgggtacaaa tcatcccggt gcgccgcccg agtcctgaac
     3721 ctagttgatc gcccccggtt cgcgcagctg accgcgcccc ggctgacctc tcctagctga
     3781 agccccgaaa gcaccagccg agacttcgac ccgtcgtttc cgagacggtc gaacggcttg
     3841 gctggttcct tcctggttcg cgtgtcgctt cggcgcgcca accctcgggt tgtgcacagg
     3901 ggggtgtatg atctcctgct acgttcgtct tacttgtcag tgaaaggaat gccgaatggc
     3961 cgtgttgtct cgtccggttt ggtcaaatac tttgctgatt ctattgtcaa cgctggtcac
//
LOCUS       DASTKU010000457        12941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_86675,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000457 DASTKU010000000
VERSION     DASTKU010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12941
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86675"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VFQ61_15645"
     CDS             <1..1307
                     /locus_tag="VFQ61_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955943.1"
                     /translation="RWSGLALCAALSIGWAFRLPRLKRDEYVGFACGVVLTAIVGAGL
                     LLGHLTRLSQASALLRFSFAHSKSSLPAAERWVALEAWFGEAGLLGLVLAALGGLVLM
                     RVARERRLACAFLVFLAFDLFVPGRSGGLAPDNAGGLRLMAVLGLVIFSVACARHVLE
                     AIERLRVPFARVAAAGALAYALILLLIGVDQGQSRGREDEEIAAATFTSEALASLPAG
                     SALLLRSEAVWRRMLTAQAVSGLRSDLSVVPMPVVESGSINAEAYAGHTGLLPLVRDM
                     LLSGKPSEYALSALADARPTYVELDPAWDLRLQSHLAPHGFFLAFAPQPLARSERAAE
                     PPRVRRGLLRVAARVRPGSGPRAAMAQGADAQRGAGRAVVDALARRAALLAGLGDLEP
                     AQQELRTLLEISPSDRGSLTLKQRLDRTPKGRVETRDLFAAR"
     gene            1389..2417
                     /locus_tag="VFQ61_15650"
     CDS             1389..2417
                     /locus_tag="VFQ61_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955944.1"
                     /translation="MFSSEMHSSTQMGNPGELRRTRRIRVARWGYVGLIGAVLGLGCQ
                     PATPPAESAANASTGSGADEPGTGTTPDAAETGAQGHAASASASGDASKPSDTSKPGG
                     AAAGGASASTPPTLVQLCEQGCTKISKSCSETAVETCRMNCTQYEHPPAGCEEDVRKS
                     LECARDAPDVTCVNIAPESCAYEFRRVVACANGKPMPAQEDPLKPPDGWERFASKSAG
                     FSAMLPKGVTESTVDGVQTFTVKQSDATYSVRLLPPPKEKPTQKNLVFVAYKIVGTCN
                     RNLKIYGLFEKPDRVFTRSDANCPDGGEYRGALVITPSKMYALSVLSPKGAKPPRDPF
                     LYAFELNH"
     gene            2508..2594
                     /locus_tag="VFQ61_15655"
     tRNA            2508..2594
                     /locus_tag="VFQ61_15655"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2542..2544,aa:Ser,seq:tga)
     gene            3200..4786
                     /locus_tag="VFQ61_15660"
     CDS             3200..4786
                     /locus_tag="VFQ61_15660"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9955945.1"
                     /translation="MLVSAHSSKVLAPGSLIEGKYRVVRQIGAGGMGAVFEAEAVRLH
                     KRVAIKVLFADGGQDEEVARRFQLEAQAAGRIGSRHIVEVIDLGQLESGAPFMVMEFL
                     DGESLRGRIARGRMQPREILPIASQLLAGLIAAHQAGIVHRDLKPDNVFLVRQTDGAG
                     DFVKLLDFGISKFNTGVGMSPTLTRTGTVLGTPYYMSPEQASGGKSIDHRVDIYAVGV
                     ILYEALTGTVPFQAETFNELLFKIVLEHARPVSSIVPEADPHFIALIERAMARDPAHR
                     FQSAADMRAALDAWSRGDASVPTFMATPNPNQFDRLSAPGSKPNYFQSTPYQSTPGSW
                     ARNSNRLDEPEEPPRRRWWPWGLGLVGVAAIAGGLIFAIQKRPDVEGAKPQGTLGAEN
                     LGTAPKATSAPPPEITPPPTINVPAATSEPGAADGPTEPTQAVSDAPVASNPTNKGGK
                     PFVRPGKRGNAIRESDSAKAGASKSEASKTETATKAPASTPTAAAPAVSPPTPAPAAP
                     PQQPATPPTKAGGRPIRVDL"
     gene            4924..5985
                     /locus_tag="VFQ61_15665"
     CDS             4924..5985
                     /locus_tag="VFQ61_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955946.1"
                     /translation="MKSNQWLLVLMLGCAVAGSAGPAAAQERISEEARNYFRNGVDLL
                     QSNPPNYQDAYYQFKLAYEKSKSWKVLGNLGLCAVKLERDGEALKYYDDYLKGGGKQI
                     DKEEREAIERDMLLIRGNTAAVELSSPAGEIEVRDLRAGSSAVAQSYQLEGGKQTLLL
                     RAGSHTLTAINKEGRELRWDIVLTPGRSVSHVFDFDAPVASPATTTESPPGVVGPPPA
                     PPEPIKPPSSGSWFSPLRITGMVATGVGLGVIVGGVVTGIKAKDREADGDAKCNKVLP
                     DGSLECETSAAQDFDDASKLAKTANILYVTGGVLAGAGIGLMIFGGGTSPEVAKARPL
                     KLTPVIGMGGGALFASGTF"
     gene            complement(6103..8772)
                     /gene="tssH"
                     /locus_tag="VFQ61_15670"
     CDS             complement(6103..8772)
                     /gene="tssH"
                     /locus_tag="VFQ61_15670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HET9955947.1"
                     /translation="MSEISRANLFGKLNEFGYKGIESATLFCKMRGNPYVELSHWLHQ
                     LLQVSDSDLLRVLRHFQVDSARLARELTEALDRLPRGATSVSDLSVQVEESAERAWVY
                     TSLLFGEAKIRTGHLVVGWIKTPNLRNAIFAISKEFQKIKVDELIDRFGTITDGSPEA
                     RLAAKLGAQPSTGDGGEIAPAALGKQEALQRYSVDLTERARNGEMDPILGRDEEIRQI
                     IQILLRRRQNNPILTGEAGVGKTAVVEGFAQRIAAGDVPPALKDVTLRALDIGLLQAG
                     ASMKGEFEGRLRQVIDEVQSSPKPIILFVDEAHTLIGAGGSQGTGDAANLLKPALARG
                     KLRTIAATTWAEYKKYIENDPALTRRFQVVKVEEPSEDKAILMLRGAVTVLEKHHRVQ
                     LLDEAVEAAVKLSKRYIPDRQLPDKAVSLLDTSCARVAVSQHAVPPEVEDSRRRIDAL
                     QAESQIIVRERNIGIDPGEREQEVRDALDAEKKRLETLETRWRSEKALVDRILAIRGE
                     LRAPDPNAPEKNKTEADPEKRTALIAELQGLNAELLQIQAEDPLILPTVDGQSVATVV
                     SDWTGIPTGRMVKNEIESVLRLHEVLGQRVIGQGHALEMISRRIQTARAGLENPSKPI
                     GVFLLAGPSGVGKTETALTLAEALYGGEQNVITINMSEFQEAHTVSTLKGAPPGYVGY
                     GEGGVLTEAVRRKPYSVVLLDEVEKAHSDVHEIFFQVFDKGWMEDGEGVRVDFKNTVI
                     LLTSNVGSDVIMKQCSGPTRPDSTALAESLRKPLLEVFPAALLGRLVVIPYYPLSDEM
                     LGSIVRLQLDRIKSRVTERYRVPFSYDDSVVKLITSRCTELESGGRMIDAVLTNTLLP
                     QVSRELLLRTLANKPVMRVHVSAENSEFVYSFD"
     gene            complement(8886..9902)
                     /gene="tssG"
                     /locus_tag="VFQ61_15675"
     CDS             complement(8886..9902)
                     /gene="tssG"
                     /locus_tag="VFQ61_15675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HET9955948.1"
                     /translation="MSLFESLKRAPYAYDFHVALRRVETLFRNRPRFGETVRPSDEPL
                     RLGQDPTMAFTPSVVADFKPPAEEGGPARLVVTFLGLFGSQGPLPTHLTEYARDRIRH
                     SGDRTFAAFVDVFHHRMLSLFHRVWGSVEPTVAEDRPETSRFPAYVGSLAGLGLKSAS
                     RRDCLPDRAKLQFSALLADPVRHPEGLRGMVQSFFELPTGVEEFVGEWLDLPDNARWR
                     LGGSPWVSSLGETSIIGKRVFRRDHKFRVVLGPLSNDDFERFLPGSESLERLKALVRA
                     YVGDQYDWDVRLVAAPDAMTSLCLARRGRLAWNARVGPRVGQQRREDLIVDPITYQTR
                     RLLA"
     gene            complement(9899..11800)
                     /gene="tssF"
                     /locus_tag="VFQ61_15680"
     CDS             complement(9899..11800)
                     /gene="tssF"
                     /locus_tag="VFQ61_15680"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HET9955949.1"
                     /translation="MDQRFLSYYNRELAYLRELGGEFAKAFPKIAGRLGLDAFECADP
                     YVERLLEGFAFMAARIQLKIDSDFPRFTEHLLSIAYPHYLAPTPSMAVVKLEPSYKQG
                     ALTEGFNVPRDTALRSNTGRGDQIPCEYRTAHPVTLWPIELVAVSHSAYVGDLGDLNV
                     ANPAPVRSALRFRFRTLSGVPFSALSLDRLPLFVRGADSLAFRTYELCCAGALGLVIR
                     SAERPAGDKGAPEKYTTELVRENGVRQMGFEDSEALLPFGPRSFQGYRLLHEYFALPT
                     RFMFFELANLGPALSRCRSSEVEIVVLLDRYSPQVEAAITPSQFELFCTPAINLFPKK
                     IDRIHLNDKEHEYHVIADRTRPLDYEVHSVNEVVGFGNSATMQREFKPFYSCTERTMH
                     DTHTAYYTLHRQPRLLSSKSRATGPRSSYTGSELFISLVDGNAGPYNSDLKQLALEAL
                     CTNRDLALHMSVGEGRTDFVMDSGAPVESVRCVAGPSEPRPSHAWGTTSWRLISHLSL
                     NHLSITDTDPEQGATMLREMLHLYGDLGGAASHREIEGMRSISTNSTVRRLPIPGPAS
                     FSRGLEITLTCDENAFEGGSAFILGSVLERFFAKHAALNSFTETVLRSQQRGEIMRWP
                     ARLGRGPLA"
     gene            complement(11809..12321)
                     /gene="tssE"
                     /locus_tag="VFQ61_15685"
     CDS             complement(11809..12321)
                     /gene="tssE"
                     /locus_tag="VFQ61_15685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HET9955950.1"
                     /translation="MAELTPKERLQPSLLDRLTDEEPDRKQESRERRIISPQRLRESV
                     RRDLTWLFNTTNMTALHPVDGYEHAERSTLNFGIPDFTGRTAGSINPQLLERELRTAI
                     WDFEPRLNKDSVRVKLIVDRERMNHNAMCFSIEAELWAQPIPLRLFLRTDLDLETGEA
                     VVEDSGGDSE"
     gene            complement(12518..>12941)
                     /locus_tag="VFQ61_15690"
     CDS             complement(12518..>12941)
                     /locus_tag="VFQ61_15690"
                     /inference="COORDINATES: protein motif:HMM:NF040147.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HET9955951.1"
                     /translation="DMAVFIGAQSLHKPAEYDDPDATANAQLSARLPYLFASCRFAHY
                     LKCIVRDKIGSFKGPDEMQRWLQKWILQYVDGSPEHSTEEAKARRPLAAAEVIVEPDE
                     SNPGYYNSKFFLRPHYQLEGLTVSLRLVSKMPSAKGGA"
BASE COUNT         2263 a         4115 c         4206 g         2357 t
ORIGIN      
        1 cgcgctggtc aggcttggcg ctctgtgccg cgttgagcat cggctgggcc tttagactgc
       61 cgcgtctgaa gcgtgatgag tacgtgggct ttgcgtgcgg ggtggtgctc actgcaattg
      121 taggcgccgg actactgctc gggcatctca cccgtctgtc gcaagccagc gcgttgctgc
      181 gcttctcgtt cgctcactcc aaatcgtctt tgccagccgc cgagcgctgg gttgctctgg
      241 aagcatggtt cggcgaagcg gggctgctgg gactagtctt ggcggcgctg ggcggcttgg
      301 tgctgatgcg ggtggcgcgg gagcgccgcc tggcctgcgc gttcctcgtt ttcctggctt
      361 tcgatttgtt cgtgcccggg cgttcgggtg ggttggctcc cgataacgcc ggcgggctgc
      421 ggctcatggc cgtgctcggc ttggtgatct tcagcgtcgc gtgcgcgcgt cacgttttgg
      481 aagcgatcga acggctgagg gtcccgtttg cgcgagtagc tgctgccggt gcgctggcgt
      541 acgccttgat tttactgctg atcggtgtgg atcagggcca gagccgcggg cgagaagacg
      601 aagagatcgc ggccgctacg ttcaccagcg aggcattggc cagcctaccc gcgggctctg
      661 cgctactgct gcggtccgaa gcggtttggc gacgcatgtt gaccgcgcaa gccgtttccg
      721 ggttgcgctc cgacctgagc gtagtgccaa tgccggtggt ggagtcaggt agcatcaacg
      781 ccgaggcata cgcgggacat acgggcttgc tgccgctggt ccgcgatatg ttgctctccg
      841 gcaagccaag tgagtacgcg ctctcggccc tggccgatgc gcgaccaaca tacgtcgagc
      901 tcgacccagc ttgggacctc aggttgcaaa gccacttggc cccccacggc ttctttttgg
      961 ccttcgcgcc gcagccgttg gctcgctccg agcgcgcggc cgagccgccg cgcgtgcgtc
     1021 ggggcctgct gcgtgttgct gcgcgagtgc gcccggggag tggaccgcga gctgccatgg
     1081 cccagggcgc ggacgcgcag cggggggcgg gtcgcgccgt ggtagatgct ctggctcggc
     1141 gggcggcgtt actggccggg ctcggagact tggagccggc gcaacaagag ctgagaacgt
     1201 tgctcgaaat ttctccaagt gatcgcggct cactcacact gaagcaacga ctggatcgca
     1261 cacccaaggg tcgcgtcgaa acccgcgatc tattcgcggc acgttgatcc tgccgacggc
     1321 tttttgcgca ttcgaaatct ctcccacccc gcaggcgacc aaacctccac cgtaacaggc
     1381 acctcgatat gttctcctct gaaatgcact ccagcaccca gatgggcaat cccggcgagt
     1441 tgcggcgcac gcgccggatt cgcgtcgctc gctggggcta cgtcggcttg atcggtgccg
     1501 ttctgggact cggctgccaa ccggcaacgc cgccggccga gagcgcggcg aacgcctcga
     1561 cgggctcggg tgccgacgag cctggcaccg ggaccacgcc tgacgcggcc gaaaccggcg
     1621 cccaaggtca cgccgcctct gcgtcagcta gtggcgatgc ttcgaagcca agcgacacct
     1681 cgaagcctgg cggcgcggca gccggcgggg cgagtgcatc cacgccgccg acgctcgtgc
     1741 aactgtgcga acagggctgc accaagatta gcaagagctg cagcgagacg gccgtggaga
     1801 cgtgccgcat gaactgcacg cagtacgagc atccccccgc gggctgcgag gaggacgttc
     1861 gcaagtcgct cgagtgtgca cgcgacgcgc cagatgtcac ttgcgtcaac atcgctcccg
     1921 agagctgcgc gtacgagttc agacgagtgg tggcttgtgc caacggtaag ccgatgccgg
     1981 cccaagagga ccctctaaag ccgccggacg gttgggagcg cttcgcgtcg aagagcgcgg
     2041 gcttctccgc aatgctgccc aaaggggtga ccgagagcac cgtggacggc gtacagacct
     2101 tcaccgtcaa acaaagcgac gcgacgtact ccgtccgttt gctgcctccg cccaaggaaa
     2161 agccgactca gaagaacttg gtcttcgtcg cgtacaagat cgtcggcacc tgcaaccgca
     2221 acctgaagat ctacgggctg ttcgagaaac cagaccgcgt cttcactcgt tccgacgcca
     2281 attgcccgga cggtggcgag taccggggtg cgctcgtgat taccccatcc aagatgtacg
     2341 ccttgtccgt gctcagcccg aaaggcgcca aacccccgcg cgaccccttc ctgtacgcgt
     2401 tcgagctgaa ccactgaagc tgatcccgaa gttgggcgtg cctgaaaaaa ggttgacgcc
     2461 gggggcgaga cggcccaaag ccatcgcgtc cgtcccgcac cttccccgga ggagtggccg
     2521 agcggtttaa ggcaccggtt ttgaaaaccg gcgtactcgc aagggtaccg tgggttcgaa
     2581 tccctcctcc tccgccgtta gcgttctgtg cgcccgctca gcatcggccc cgcgagcacg
     2641 ccccgccgtc ggcgatcggc cgcggttgag agttgctgtg ttggcgagct gcgagaggtc
     2701 ggtcgttttt gctcaaacgc caccgcgaat tggcgcgaac gtggaggtct cgcgagcgaa
     2761 gacgaaaaac cgggaaggcc gcgagggagg gcggcgtaca agagcgctga ggccggctcg
     2821 gctatgaagc tcggagcttt cccgcgctgg cgagccccgg cgaccagggt cgggcgagtt
     2881 gcctggacgt ctgcccagca cgctgcgcac taggattcgg ctttggcgca catcgttgag
     2941 agaaatcccg gctgcgagct ggattgggtt tcggcctcgg tgttcgagac acccaatgag
     3001 gtgaaggttc gaagggttac acggcgatga gtaccgcaga aacacggcga ttcgagcgag
     3061 ccatctcgat ccgagctggc gtgcttcacg cgcaagcggc attggcgtgg gcggtaaccg
     3121 tactggcacg accagtggcg tcggcgttgg aggatcgcgg cgtgtttcgg tccgggcgct
     3181 cgcccgcttc ggtgacaccg tgctagtctc cgcacactcg tccaaagtgc tggcccccgg
     3241 ttcactcatc gaaggtaagt accgcgtcgt tcgccagatc ggggcaggcg gcatgggtgc
     3301 ggtgttcgaa gcggaagccg ttcggctgca caagcgcgtc gccatcaaag tgttgttcgc
     3361 ggacggcggc caggacgaag aggtggcgcg tcgctttcag ctcgaagctc aagccgcggg
     3421 acgcatcggt tcacgccaca tcgtggaggt catcgatctg gggcagctcg agtctggggc
     3481 gcccttcatg gtgatggagt ttctagatgg cgagagcctg cgcgggcgaa tcgcgcgcgg
     3541 gcgcatgcag ccgcgcgaaa tcctgccgat cgcttcgcag ctgctcgcgg gtctaatagc
     3601 ggcgcatcag gccggcatcg ttcaccggga tttgaagccg gacaacgtgt tcctcgtgcg
     3661 gcagacggac ggcgcgggag acttcgtcaa actcttggat ttcggcattt cgaagttcaa
     3721 cacgggcgtg ggaatgagcc ccacgctgac gcgaaccggc acggtgctgg ggacgcccta
     3781 ttacatgtcc ccggagcagg cgagcggcgg caaatccatc gatcaccgcg tagacatcta
     3841 tgccgtcggg gtgatcttgt acgaggcact caccgggacg gtgcctttcc aggccgagac
     3901 cttcaacgag ctgctgttca agatcgtgct cgagcacgcg cggccagtgt cgtcgatcgt
     3961 accggaggcg gatccgcact tcatcgcgct gatcgaacgc gccatggctc gagatcccgc
     4021 ccaccggttt cagagcgcgg ccgatatgcg cgcggccctg gatgcttggt cgcgcggaga
     4081 tgcctccgtg ccgacgttca tggccacccc gaacccaaac cagttcgatc gcctatcggc
     4141 gccgggcagc aaaccgaact acttccagtc cactccgtac caatccactc ccggcagctg
     4201 ggcgcgcaac agtaatcgct tggatgaacc cgaagagccc ccgcggcgac gttggtggcc
     4261 ttggggtctc gggctcgtag gggtcgcggc cattgcgggt gggctgatat tcgcgattca
     4321 gaagcgcccg gacgtagaag gggccaagcc gcaaggcaca ctgggagccg agaacctcgg
     4381 taccgcaccc aaggctacga gcgctccgcc gccggagatc acgcccccgc ccaccatcaa
     4441 cgtacccgcg gccacctccg aaccgggcgc ggcggacggc ccgactgagc ctactcaggc
     4501 tgtttccgac gctccggttg cgagcaaccc gacgaataag gggggcaagc ccttcgttcg
     4561 tccaggcaag cgcggcaacg ccattcgaga gtcggattcc gcaaaggcgg gggcttcgaa
     4621 gtcggaagcg agcaagaccg aaacggctac caaggctccg gcgtcgaccc ctacggccgc
     4681 cgcacccgca gtttcaccgc ccaccccggc acctgcggcg ccgccgcagc agcctgccac
     4741 gccgcctacc aaggcgggcg ggcgcccaat ccgcgtagat ctttgagctg agccgctcct
     4801 gctggaatag cggcggtcgt cccaggagga gcgcagagct ttcgggtccg gaagcttgtg
     4861 gtccgcgccc gcttcgaata cttgaaaggg cggagccctt ttccgttact cgggcagcgt
     4921 gtgatgaaat ctaaccagtg gcttttggtg ttgatgctgg ggtgtgccgt ggctgggagt
     4981 gcgggtcccg cagcggcgca ggaacgcatc tccgaagagg cgcggaatta cttccgcaat
     5041 ggcgtcgatc tgctgcagtc caatcctccc aattatcaag acgcctacta ccagttcaag
     5101 ctcgcatacg agaagagcaa gagctggaag gtgcttggta acctcgggct gtgcgcggta
     5161 aagctcgagc gcgacggcga agctctcaag tactacgacg actacctgaa gggcggcggc
     5221 aagcagatcg acaaggaaga gcgcgaggcg atcgagcgcg acatgctgct gatccgcggc
     5281 aacaccgccg ccgtcgagtt gtcttcaccg gccggcgaga tcgaggtgcg tgatttgcgt
     5341 gccgggtcca gtgccgtggc gcagagctat cagctcgagg gcggcaaaca gacgctgctg
     5401 ttgcgtgccg gctctcacac ccttacggcc atcaacaagg agggccgcga gctgcgctgg
     5461 gacatcgtgc tcacgccggg gcgcagtgtc tcgcacgtgt tcgacttcga cgccccggtt
     5521 gcgagcccgg ccactactac cgagtccccg ccgggtgtcg tgggtccgcc gcctgcgccg
     5581 ccagagccga tcaagccacc gagctcgggc agttggttct caccgctgcg tatcaccggt
     5641 atggtcgcta cgggcgtcgg cctgggtgtt atcgtgggcg gcgtcgtgac tggcatcaaa
     5701 gccaaggacc gcgaggcgga cggcgatgcc aagtgcaaca aggtgcttcc ggatggctca
     5761 ctcgagtgcg agacctcggc ggcgcaagac ttcgacgacg cctccaagct cgccaaaaca
     5821 gccaacatcc tttacgtcac cggcggcgtc ttggccggcg cgggcattgg cttgatgatc
     5881 ttcgggggcg ggacttcgcc ggaagtcgcc aaggctcggc ctctgaagtt gacgcccgtg
     5941 atcggtatgg gtggcggcgc actgttcgcg agcggcacct tctgagcttt tgcggttcgc
     6001 gagtggccat gccccgggcc cgttcgttcg aggggatgca tcgaaaaaga aagggcttcg
     6061 agacgcgatc tcgaagccct tttcgcttac ctcatcgagg tctcagtcga agctgtacac
     6121 gaactccgag ttctcggcgc tcacgtgcac gcgcatcacc ggcttgttcg cgagcgtacg
     6181 caacagcaac tcacggctga cctggggcaa tagcgtgttg gtcagcaccg cgtcaatcat
     6241 gcgaccgccg ctctcgagct cggtgcaacg gctggtgatc agcttgacca cgctgtcgtc
     6301 gtagctgaac ggcacgcgat agcgctccgt cacgcggctc ttgatgcgat cgagctgcag
     6361 acggacgatc gagccgagca tctcgtcgct caacggataa tacggaatca ccaccaagcg
     6421 gccgagcaac gccgcgggga agacctcgag caaaggcttg cgcagcgact ccgccaacgc
     6481 ggtcgagtcg ggccgcgtgg ggcccgagca ttgcttcatg atcacgtcgc tgcctacgtt
     6541 cgaggtcagc aggatgacgg tgttcttgaa gtcgactcgg acgccttcgc cgtcttccat
     6601 ccagcctttg tcgaagacct ggaagaagat ctcgtgcacg tcggagtgcg ccttctccac
     6661 ctcgtcgagc agcaccacgc tgtacggctt gcgtcgcacc gcctcggtga gcacgccgcc
     6721 ttcgccgtag ccgacgtatc cgggcggcgc gcccttcaac gtcgacacgg tgtgcgcctc
     6781 ctggaattcg ctcatgttga tggtgatgac gttctgctcg ccaccgtata gagcttcggc
     6841 cagcgtgagc gccgtctccg tcttgccgac gcccgagggc cccgcgagca agaacacacc
     6901 gattggcttg ctggggttct cgagcccggc gcgtgccgtc tgaatgcggc gcgagatcat
     6961 ctcgagcgca tggccctggc cgatcacgcg ctgaccgagc acctcgtgca aacgcagcac
     7021 ggactcgatc tcgtttttga ccatgcgtcc cgtgggaatg cccgtccaat ccgacaccac
     7081 ggtggcgact gattggccgt cgaccgtcgg caggatcagc gggtcttcgg cctgaatttg
     7141 cagcagctcg gcgttcaagc cttgcagctc cgcgatcagc gccgtgcgct tctccggatc
     7201 ggcttcggtc ttgttcttct cgggagcatt cggatcgggg gcgcgcagct cgccgcggat
     7261 cgcgaggatg cgatcgacca aggccttctc cgagcgccag cgcgtctcga gcgtctcgag
     7321 ccgcttcttc tcggcgtcga gcgcgtcgcg tacttcttgt tcgcgctccc cgggatcgat
     7381 gccgatattg cgctcgcgca cgatgatttg cgactcggcc tgcagcgcgt cgatgcgccg
     7441 acggctgtcc tcgacttcgg gcggcaccgc gtgttggctc accgctacgc gcgcgcacga
     7501 agtatcgagc aggctcacgg ccttgtccgg caactgccga tccgggatgt agcgcttcga
     7561 gagcttgacg gcggcttcca ccgcctcgtc gagcagctgc acgcggtggt gcttctcgag
     7621 cacggtgacc gcgccccgca gcatcagaat ggccttgtct tcgctcggct cttctacctt
     7681 cacgacctgg aagcgacgcg tgagcgccgg gtcgttctcg atgtacttct tgtactcggc
     7741 ccaagtggtc gccgcgatcg tgcgcagctt gccgcgcgcc agcgctggct tgagcaaatt
     7801 ggctgcatct ccggtgccct gcgagccacc cgcaccaatc agggtatgcg cctcgtccac
     7861 gaacaagatg atgggtttgg gcgacgattg cacttcgtcg atcacctggc gcaggcggcc
     7921 ctcgaactca cccttcatgc tcgcgcccgc ttgcagcagg ccgatatcga gcgcgcgcag
     7981 ggtcacgtct tttagcgctg gcgggacgtc gccggcggcg atgcgctggg caaagccttc
     8041 gacgacggcc gtcttgccga cgcctgcctc acccgtgagg atggggttgt tctggcgcct
     8101 tcgcagcaaa atctggatga tttgccggat ctcttcgtcg cgtccgagga tgggatccat
     8161 ctcgccgttg cgcgcgcgct cggtgagatc gaccgagtaa cgttgcagcg cttcttgctt
     8221 gccgagcgcc gccggtgcga tctccccacc atcgcccgtg ctgggctgag cgccgagctt
     8281 ggcggcgagc cgagcctcgg gggagccgtc ggtgatcgtg ccgaagcggt cgatcaactc
     8341 gtcgaccttg atcttctgga actccttcga gatcgcgaag atcgcgttcc gcaggttcgg
     8401 tgtcttgatc caacccacca ccaaatggcc ggtgcgaatc ttggcttccc cgaacagcag
     8461 cgaggtgtaa acccaggctc gctccgcgga ttcctcgacc tgcaccgaaa ggtcggagac
     8521 cgaagtggct ccgcgcggta gtcgatcgag ggcctcggtc aactcgcgcg caagccgggc
     8581 cgaatcaacc tgaaaatggc gcaggacgcg cagcaagtcg gagtccgaaa cttgcagcag
     8641 ctggtgcaac caatgggaaa gctcgacgta agggttgccc cgcattttgc agaacaacgt
     8701 ggcgctctcg atgcccttgt agccgaactc gttcagcttc ccgaacaggt tcgcacgact
     8761 gatttccgac atgcatcact cctcattgac ggggtgtttc accccgatcc cataccgaag
     8821 gccccctgga gatctggttt ggcctctgaa atccgagccc gcatcatagc cgctgcccct
     8881 gcgggctacg cgagcaatct gcgtgtttgg taagtgattg gatccacgat caagtcttca
     8941 cgccgctgtt gccccacgcg cggccctacg cgagcattcc aagccagccg cccccggcgg
     9001 gccaagcaca gcgaggtcat cgcgtcggga gccgcgacta ggcgcacgtc ccagtcgtac
     9061 tggtcgccga cgtacgctcg aaccaaggct ttgagccgtt cgaggctctc gctacctggt
     9121 aaaaagcgct cgaagtcgtc gttcgagagc ggcccgagca cgacccggaa tttgtgatct
     9181 ctgcggaaca cgcgcttgcc gatgatcgac gtttccccga gcgagctcac ccacggcgaa
     9241 ccccccaggc gccagcgcgc gttgtccggt aaatccagcc actcgccgac gaactcttcg
     9301 acgccggtcg gcaactcgaa gaagctttgc accatgccgc gcagcccctc ggggtgacgg
     9361 acgggatcgg cgagcagggc ggagaattgg agcttggccc gatcgggcaa gcaatctcgg
     9421 cgcgaggccg acttgaggcc gagaccggcg agcgaaccca cgtacgcggg gaagcgactc
     9481 gtctccggtc gatcttcggc cacggtgggt tccacgctgc cccacacgcg gtggaacagc
     9541 gagagcatgc gatggtgaaa cacgtccacg aaggcggcga acgtgcgatc tcccgagtgt
     9601 cggatgcggt cccgagcgta ctcggtcagg tgcgtcggga gtggcccctg cgaaccgaac
     9661 aagcccaaga acgtgaccac tagccgcgcc ggcccaccct cttcggcggg cggcttgaag
     9721 tccgccacca ccgagggggt gaatgccatg gtcggatctt gcccgagccg cagcggttcg
     9781 tcgctcggtc gaactgtctc cccgaaacgt ggtcggttcc gaaagagagt ttctacgcgt
     9841 ctcaacgcaa cgtggaaatc gtaggcgtag ggcgcgcgct tcagtgactc gaacaggctc
     9901 acgcgagcgg ccctctacct agtcgcgcgg gccaccgcat gatttctccg cgctgttggc
     9961 tgcgcaagac ggtctcggtg aaagagttga gcgccgcgtg cttggcgaag aaccgctcca
    10021 gcaccgagcc cagaatgaac gcgctgccgc cttcgaacgc gttctcgtcg caggtcagcg
    10081 tgatctcgag cccacgggaa aagcttgccg gaccggggat tgggagccgg cgcaccgtgg
    10141 agttggtcga aatcgagcgc atgccctcga tttcacggtg tgacgccgcg ccgcccaaat
    10201 cgccgtacag gtgcaacatc tcccgcaaca tggtggcgcc ctgctcggga tccgtatcgg
    10261 taatcgagag gtgattcagg gacaggtggc tgatcaaacg ccaagaagtg gtgccccagg
    10321 catgggacgg ccgtggctcc gatggccctg ccacgcagcg caccgattcc actggcgctc
    10381 ccgagtccat cacgaaatcg gtgcggccct caccgacgct catgtgcaga gccaggtcac
    10441 ggttggtgca caaggcctcg agcgccagct gcttcagatc cgagttgtag gggcccgcgt
    10501 tgccgtccac tagcgaaatg aagagctcgc tgccggtgta gctcgagcgt ggcccggttg
    10561 cccgcgactt gctcgacagc agacgcggtt gccgatgcag cgtgtaatac gccgtgtgcg
    10621 tgtcgtgcat cgtgcgctcc gtgcacgaat agaacggctt gaattcccgc tgcatagtag
    10681 cgctgttgcc gaagccgacc acttcgttga ccgaatgtac ctcgtaatcg agcggtcgcg
    10741 tgcgatccgc gatcacgtgg tactcgtgct ctttgtcgtt cagatggatg cggtcgatct
    10801 tcttggggaa caggttgatg gccggcgtgc agaacaactc gaactggctg ggcgtgatcg
    10861 cggcttcgac ctgcggcgaa tagcgatcga gcagcaccac gatctcgacc tcggagctgc
    10921 ggcagcggct gagcgccggc cccagattcg cgagctcgaa gaacatgaag cgggttggca
    10981 gcgcgaagta ctcgtggagc agccgatagc cttggaacga tcgcggcccg aacggcagca
    11041 gcgcctcgct gtcctcgaaa cccatttggc gaacgccgtt ttcgcgcacg agctcggtcg
    11101 tgtacttctc gggagcgccc ttgtccccgg cgggccgctc tgcgctacga atcacgagcc
    11161 ccagcgcgcc tgcgcagcac agctcgtacg tgcggaacgc cagcgagtcc gcgccgcgca
    11221 cgaacagggg aaggcggtct agcgagagcg ccgaaaaggg gaccccgctc agcgtccgga
    11281 agcggaaacg gagcgcagag cgcaccggcg ccgggttcgc gacgttcaaa tcccccaagt
    11341 caccgacgta cgccgagtga ctgacggcca ccagctcgat cggccacagt gttacggggt
    11401 gtgcagtgcg gtattcacac ggaatctgat cgccgcggcc cgtgttgctg cgcagcgccg
    11461 tgtcccgggg aacgttgaag ccctccgtga gcgcgccttg cttgtagctc ggctcgagct
    11521 tcacgaccgc catcgacggg gttggcgcga ggtaatgtgg atacgcgatc gacagcaggt
    11581 gctcggtaaa gcgcgggaaa tcggagtcga tcttgagctg gatgcgcgcg gccatgaacg
    11641 cgaagccctc gagcaaacgc tccacgtagg gatccgcaca ctcgaaagcg tccaatccga
    11701 gccgcccggc gatcttcgga aacgctttcg cgaactcacc cccgagttca cgaaggtacg
    11761 ccagctctcg gttgtagtag gagagaaaac gttgatccat ttacgacttc actcggaatc
    11821 tccgccgcta tcttccacga cggcttcacc ggtttccaag tccagatcgg tacgcaaaaa
    11881 tagacgcaaa ggaatgggct gcgcccagag ctcggcttcg atgctgaagc acatggcgtt
    11941 gtgattcatg cgctctcggt cgacgatcag cttcacgcgc accgaatctt tgttgaggcg
    12001 cggctcgaaa tcccagatcg cggtgcgcag ctcacgctcc aacaactgtg ggttgatgct
    12061 gccggcggtg cggcccgtga aatccgggat gccgaaattc agcgtcgaac gctcggcgtg
    12121 ctcgtagcca tccacgggat gaagcgccgt catgttcgtg gtgttgaaca accacgtcag
    12181 atcgcgacgc acactttccc gcaggcgctg gggcgaaatg atgcgtcgct cacgcgactc
    12241 ttgcttgcgg tccggctctt cgtcagtgag ccggtcgagt agcgacggtt gtaggcgttc
    12301 ttttggggtc agctcggcca tcgtgtctcg aagcagcctt gttggatcga ccgaggcacg
    12361 caggtgcagc cctgttcccg aggtggtctc tcgagcccga gctggctgcg ctgcgtaccg
    12421 aacgcgctgg gcgacgcagt tggtggcgtc acccagcgca gtcagcggtc aggaccgtat
    12481 cagcggtgcc gttccgtgga tggttcgcgc gcgcccgtta ggcgccgccc ttggcggacg
    12541 gcatcttgga taccaggcgc aaagacaccg tgaggccttc gagctggtaa tgcgggcgca
    12601 ggaagaactt cgagttgtag taaccggggt tgctctcgtc tggttcgacg atgacctcgg
    12661 cggcggcgag cggtcggcgc gccttggcct cttccgttga atgctcgggg ctgccgtcga
    12721 cgtactgcag aatccacttt tgcagccagc gttgcatctc gtccggccct ttgaaggagc
    12781 cgatcttgtc gcgcacgatg cacttcagat agtgcgcgaa gcggcagctg gcgaagaggt
    12841 acggcagacg cgccgaaagc tgcgcgttcg ccgtcgcatc cggatcgtcg tactctgccg
    12901 gcttgtgcaa cgactgcgcg ccgatgaaga cggccatatc g
//
LOCUS       DASTKU010000458         5253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_978933, whole genome shotgun sequence.
ACCESSION   DASTKU010000458 DASTKU010000000
VERSION     DASTKU010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978933"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="VFQ61_15695"
     CDS             <1..169
                     /locus_tag="VFQ61_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET9955952.1"
                     /translation="GSVQLPEGVKMVMPGDNVTVQVELITSVALEEQMRFAIREGGKT
                     VGAGVVTQILE"
     gene            174..368
                     /gene="rpmG"
                     /locus_tag="VFQ61_15700"
     CDS             174..368
                     /gene="rpmG"
                     /locus_tag="VFQ61_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET9955953.1"
                     /translation="MASGRLSTPRVGPTHSGRIRISLSCSVCGKRNYRSTKSPRDGEA
                     LSLKKFCNQCGQHTAHIEGK"
     gene            524..596
                     /locus_tag="VFQ61_15705"
     tRNA            524..596
                     /locus_tag="VFQ61_15705"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:557..559,aa:Trp,seq:cca)
     gene            624..1196
                     /gene="secE"
                     /locus_tag="VFQ61_15710"
     CDS             624..1196
                     /gene="secE"
                     /locus_tag="VFQ61_15710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET9955954.1"
                     /translation="MATESDQNQKPKADDLSHEAEEAIAADAQVVPGLAGTVVHDSSS
                     PDEDAPATVQLGAKRYVHAAFFAAGILVAFLSSKILHAVWSELADWPAAVRAIPQLVS
                     YPEEERDGFTLGIGALIGAISVIQVYRKAAIRGWADDVAGELAKVSWPNRDTVVNGTL
                     VVVIASAFATVYVAILDKFWSFLTTLVYGA"
     gene            1208..1753
                     /gene="nusG"
                     /locus_tag="VFQ61_15715"
     CDS             1208..1753
                     /gene="nusG"
                     /locus_tag="VFQ61_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET9955955.1"
                     /translation="METAATGKKWYVVTTYSGFENKVKSALQERIRQYKMEDRFGEIL
                     IPSETVTDTAKDGKQRLKTKTSFPGYVFIEMEMSENAWHLVKDTPKVTGFIGNQRPQE
                     VKSPHIQDIRKGIVEGAVKPKPRQQFQEGDEVRVVVGAFANFSGTVQEVKPDKQKLKV
                     MVSIFGRPTPVELDFSHVEKR"
     gene            1750..2196
                     /gene="rplK"
                     /locus_tag="VFQ61_15720"
     CDS             1750..2196
                     /gene="rplK"
                     /locus_tag="VFQ61_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET9955956.1"
                     /translation="MSAKKKITAYIKLQLPAGKANPSPPVGPALGQHGVNIMQFCKDF
                     NARSAPLGDTIIPVVITVYGDRSFTFVMKSPPASVLIKKACGLPSAKKPGSGSKEPNK
                     TKVGQLTWDQVRTIAKEKIKDMNAADVEAAARSIAGTARSMGVDVV"
     gene            2284..3000
                     /gene="rplA"
                     /locus_tag="VFQ61_15725"
     CDS             2284..3000
                     /gene="rplA"
                     /locus_tag="VFQ61_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET9955957.1"
                     /translation="MASKLAKNRAAALSKIDRNKRYTVEDAVKLVKDTAYAKFDESVD
                     IAVRLGVNPKHADQMVRGAIVLPHGTGKSIRVLVFARGEKEREALDAGADVAGGDELA
                     ARVLEGFLDFDRVIATPDMMGVVGKLGRVLGPRGLMPNPKVGTVTFDLKNAVRESKAG
                     KVEYRVDKAGVVHCRIGRRSFDQQKLSENALALVTELLHKKPSTAKGTYLKSITLSST
                     MGPGVRVDTSTVVTTSEEER"
     gene            3002..3526
                     /gene="rplJ"
                     /locus_tag="VFQ61_15730"
     CDS             3002..3526
                     /gene="rplJ"
                     /locus_tag="VFQ61_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET9955958.1"
                     /translation="MDRAQKLEQIGELKTQFERMTSAVFVDFTGMTVEQVTKLRDTFR
                     AKGIEYKVVKNTLVQQALGEKPWASKLEPVLKGMTGVAWSFEEPSAAARVLKDFCKEN
                     EKLKIKAGLLEGEVLNSTAVTEQLAALPNKDEARAMLLATLMAPAQRMVMLLNAPARN
                     FVGVLAAKQRQDEG"
     gene            3653..4033
                     /gene="rplL"
                     /locus_tag="VFQ61_15735"
     CDS             3653..4033
                     /gene="rplL"
                     /locus_tag="VFQ61_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET9955959.1"
                     /translation="MAEITREQVVDYLSNLPVIQIAELVKELEGKWGVSAAPVAVAAA
                     GGGAAAAAPVEEKTEFNVILADAGANKINAIKVVREITGLGLKEAKDLVEGAPKPIKE
                     GVSKADAEEIKKKLEAAGAKVEVK"
     gene            4489..>5253
                     /locus_tag="VFQ61_15740"
     CDS             4489..>5253
                     /locus_tag="VFQ61_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET9955960.1"
                     /translation="MASTVQSNFRHRMNLGRVEPIVDIPNLIDIQKSSYDKFLQSDVA
                     PRERREMGLEAVFRSVFPIKDFDGTSELVYVSYNLEKPKYDVDECRQRGMTFAAPIKV
                     TTQLMVYDTREGGERIVRDIKEQEVYFGEIPLMTETGTFIINGTERVVVSQLHRSPGV
                     FFDHDKGKTHSSGKLLYSARIIPYSGSWLDFEFDHKDIMYVRIDRRRKMHATVLLRAL
                     AFSTQDLLNYFYSTETVYLEKTGKFAKSIEFDLLPGQ"
BASE COUNT         1073 a         1557 c         1675 g          948 t
ORIGIN      
        1 ggggagcgtg cagctacccg aaggcgtgaa gatggtaatg ccgggtgaca acgtgacggt
       61 tcaggtggag ctgatcacgt cggtggcgct cgaagagcag atgcgcttcg cgatccgcga
      121 aggcggcaag accgtcggcg ctggcgtcgt cacccagatc ctcgagtaag acgatggcga
      181 gtgggcggct ctcgacaccg cgcgtcggcc cgacgcacag tggccggatc cgcatctcgc
      241 tttcgtgttc ggtgtgtggc aagcgcaact acaggagcac caagagccct cgcgatggcg
      301 aggccctcag tttgaagaag ttctgcaatc aatgcggtca gcacacggca cacatcgaag
      361 gcaagtgatc tgaagatatc ccgcccgcta ctgcagcggg caagcgtctc gagcccgcgg
      421 ccaacgcggg ctctggcgtt ggacccgcgg ttctcgcggg cactcggtgg gcctcgcgtc
      481 agcgggatcc agcgaatgac ccgaagccgc ggggagtgcc tctaggggcg tagctcagtt
      541 ggtagagcag cggattccaa atccgcgggt cgggagttcg agtctctccg cccctgccgc
      601 agtaccgcaa ccatccgaaa cccatggcca ccgagagcga ccaaaaccaa aagcccaagg
      661 ctgatgacct cagccacgag gctgaagagg cgatcgccgc tgatgcacag gttgtccctg
      721 ggcttgcggg aacggtcgtg cacgattcgt cgtcgcccga cgaggatgcg cctgcgaccg
      781 tgcagcttgg ggcaaagcgc tacgtgcatg cagcattctt tgctgcgggc atcttggtcg
      841 cgttcttgtc gagcaagatc ctgcacgccg tgtggagcga gcttgccgat tggccggcag
      901 ccgtgcgggc tatcccccag ctcgtcagct atcctgagga agagcgtgac ggctttactt
      961 tgggaattgg cgccctgatt ggcgcgatca gcgtcattca agtttatcgc aaggcagcca
     1021 ttcgaggctg ggccgacgac gtcgccggcg agcttgcgaa ggtgagttgg ccaaatcgcg
     1081 acaccgtagt gaacggaacc ctcgtggtcg tgatcgctag cgcgttcgca actgtctacg
     1141 ttgccatcct ggataagttt tggagctttc tcaccaccct cgtgtacggg gcctaggcga
     1201 aaccaccgtg gaaactgctg cgacaggcaa gaagtggtac gtcgttacga cttactcggg
     1261 cttcgagaac aaggtgaagt ccgcgctgca ggagcgcatt cgacagtaca agatggagga
     1321 ccggttcggg gaaatcctga tcccgtccga aaccgtcacg gatacagcca aagacggtaa
     1381 gcaacggctc aagaccaaga ccagcttccc tggctacgtc ttcatcgaaa tggagatgag
     1441 cgagaacgcc tggcacttgg tcaaggacac cccgaaggtc accgggttca tcggcaacca
     1501 gcgcccgcaa gaggtgaaga gcccgcacat ccaggatatc cgcaaaggaa tcgtggaagg
     1561 cgcggtcaag cccaagcccc ggcagcaatt tcaggagggc gacgaggtgc gagtcgtcgt
     1621 cggcgccttc gcgaactttt ccggcacggt ccaagaggtc aagccggata agcagaagct
     1681 caaggtcatg gtcagcatct tcgggcgtcc gaccccggtc gagctcgatt tttcacacgt
     1741 tgagaagcga tgagcgcaaa aaagaagatt accgcataca tcaagctgca gctcccggct
     1801 ggcaaggcga atccctcgcc gccggttggt cccgcgctcg gccagcacgg ggtcaacatc
     1861 atgcagttct gcaaggattt caacgccagg agcgcgcctc tcggtgacac catcatcccg
     1921 gtggtgatca cggtttacgg cgatcgctcg ttcaccttcg tgatgaagtc gccgcccgcg
     1981 agcgtgctca tcaagaaggc gtgcgggctg ccctcggcga agaagcccgg cagtggctcg
     2041 aaggagccga acaagaccaa ggtcggacag ctcacctggg accaggtgcg cacgatcgcc
     2101 aaggagaaga tcaaggacat gaacgccgct gatgtggagg ctgccgcgcg atcgatcgct
     2161 ggcaccgccc gcagtatggg cgtcgacgtc gtttgatcgc tggtctgaag cattactggt
     2221 gcggcgtaag cggcgcgcaa gctccgcgag cgaccgtctc ccaggagtcg agagacgcaa
     2281 agaatggcta gtaaactcgc gaaaaacaga gcagcggctc tctccaagat cgaccgcaac
     2341 aagcggtaca cggtcgagga cgccgtcaag ctcgtgaaag acaccgcata cgcgaagttc
     2401 gacgagagcg tcgacatcgc tgtgcggctc ggcgtgaatc caaaacacgc cgatcagatg
     2461 gtgcgtggcg ccatcgtgct gcctcatggg acaggcaaga gcattcgcgt gctcgtgttt
     2521 gcccgaggcg agaaggagag agaggccttg gacgccggcg cagacgtggc cggtggcgat
     2581 gagctcgcgg cacgcgtgct cgagggcttc ctcgatttcg accgcgtgat cgcgacccca
     2641 gacatgatgg gcgtggtcgg taagctgggc cgcgtgctcg gtccgcgcgg tctgatgccg
     2701 aacccgaagg tcggcaccgt gacgttcgat ctgaagaacg cggtgcgtga atccaaggcc
     2761 ggcaaggtcg agtaccgcgt ggacaaggcg ggtgtggtgc attgccgcat cggtcgtcgt
     2821 tcgttcgatc agcagaagct gtcggagaac gcgctcgcgt tggtcaccga gctccttcac
     2881 aagaaacctt ccactgccaa gggtacgtac ctcaagagca tcacgctctc gagcacgatg
     2941 ggcccgggcg tgcgcgtcga cacgagcacc gtggtgacga cctccgagga ggagcgctga
     3001 gatggatcgc gcacaaaaac tcgagcaaat tggcgagcta aagacgcagt tcgagcggat
     3061 gacctccgca gtgttcgtcg acttcacggg tatgaccgtg gagcaggtga cgaagctgcg
     3121 cgatacgttc cgcgcgaagg gcatcgagta caaggtcgtc aagaatacgc tcgtgcagca
     3181 agctctcggt gagaagccct gggcgtccaa gctcgagccc gtgctgaaag gcatgacggg
     3241 cgtggcttgg agcttcgaag agccgagcgc agccgcgcgc gtgctcaagg acttctgcaa
     3301 ggaaaacgag aagctcaaga tcaaggccgg tttgctcgaa ggcgaggtgt tgaactccac
     3361 cgccgtcacc gagcagctcg ccgctctgcc gaacaaggac gaggcgcgcg ctatgttgct
     3421 cgccacgttg atggctccgg cgcagcgcat ggtcatgctg ctcaatgccc cggctcggaa
     3481 cttcgtcggc gtgctcgctg cgaaacaacg tcaggacgag ggttgagatc ttccgatctc
     3541 cccatcccat cccccaccgt acccccaaca gtttcaaaaa tatttagaac actaaccgcc
     3601 caagccaaag ggtcgacccc ttcccggatt gggaccgtat ttggagacag tcatggctga
     3661 gatcacccga gagcaggtcg tcgattacct ttccaacctt cccgtcattc agatcgcaga
     3721 gctcgtgaag gagctcgagg gcaagtgggg cgtcagtgcc gctccggttg ccgtggccgc
     3781 cgccggcggc ggtgcggccg ctgctgctcc ggtcgaagag aagacggagt tcaacgtcat
     3841 cctggccgac gcaggcgcca acaagatcaa cgcgatcaag gtcgtccgcg agatcaccgg
     3901 tctcggcctc aaggaagcca aggacctggt cgaaggcgca cccaagccga tcaaggaagg
     3961 cgtgagcaag gccgacgcgg aagagatcaa gaagaagctc gaagcggccg gcgcgaaggt
     4021 cgaggtcaag taatcacttg cccctgactc cttcaaagag tccccaagca gcgccgcggc
     4081 tttcaggccg cggcgctcgc tgcgtctgcg agggtatgat ggcccgcggg tcggcgtcta
     4141 tttgccgtgc ctgcgtgtcc gcgtttttct ttacgcgttt cttttcttgt gagcgaccgg
     4201 gccccctggc tcgaggtcgc tctttgtgca gcttggctgt gctccgcaat cggcgccaca
     4261 tgtttttggc gttcggtgag cgtgagcgcg gttcggctgc gcgtgtcgtt gatttacgcg
     4321 gttcacgtct gccttttggt ctgaggcacg gcgtggccgc tggttttgtt ggcaccagtt
     4381 tcattggcac tcggcacagc gtggctgcgc ggagtggctc ggagcgcgct tccggcgcgc
     4441 ttgggctcgc ctaacgcatt ttagtcctgc tcgtttggag gcacccagat ggcgtccacg
     4501 gtccagtcga acttccggca tcgcatgaat ctcggtcgtg tggagccgat cgtcgacatt
     4561 ccgaacctga tcgacatcca gaagagtagc tacgacaagt tcctgcagtc cgacgtggcg
     4621 ccgcgtgagc gccgcgagat ggggctggag gcggtgtttc gcagcgtatt cccgatcaag
     4681 gacttcgacg gtacgagtga gctcgtttac gtttcctata acctcgagaa gccgaagtac
     4741 gacgtcgatg agtgccgtca acgcggcatg accttcgcgg ctccgatcaa ggtgacgact
     4801 cagctcatgg tctacgacac ccgcgagggt ggcgagcgca tcgtgcgcga catcaaggag
     4861 caagaggtct acttcggcga gatcccgctg atgacggaga ccggcacctt catcatcaac
     4921 ggcaccgagc gtgtcgtcgt gtcgcagctg catcgcagcc cgggcgtgtt cttcgaccac
     4981 gacaagggca agacccactc cagcggcaag ctgctctatt cggcgcgcat tatcccgtac
     5041 agcggctcgt ggctcgactt cgagttcgac cacaaagaca tcatgtacgt gcgcatcgac
     5101 cgccgccgga agatgcacgc gacggtgctc ttgcgcgcgc tcgcgttcag cacccaagac
     5161 ctgctgaact acttctactc gacggagacc gtctacctcg agaagacggg taagttcgcc
     5221 aagagcatcg agttcgatct gttgccgggc cag
//
LOCUS       DASTKU010000459        16087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_979039, whole genome shotgun sequence.
ACCESSION   DASTKU010000459 DASTKU010000000
VERSION     DASTKU010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16087
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979039"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VFQ61_15745"
     CDS             complement(<1..1140)
                     /locus_tag="VFQ61_15745"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HET9955961.1"
                     /translation="MTNDFLKQAFVYLVAAVLGVPLAKRLGLGSVLGYLLAGVAIGPF
                     ALGLVGHDGHDVMHFAEFGVVMMLFLVGLELRPSLLWSLRRPILGLGGLQVLCTSLVF
                     GAVAFALGVPLKPALAVGMIFSMSSTAIVLQSLNERGLSHGAGGQAAFSVLLFQDIAV
                     IPILALFPLLGDGAATVSTETEPSRWVRAASVLAAVALVVLGGRYLVRPAFRWLAETR
                     LREAFTAAALMLVVGIALLMSYVGLSAALGTFLAGVVLAESEYRHELESDIEPFKGLL
                     LGLFFISVGAQIDFGYVAHHPWQVGVGVVSSMLLKLLVLYGVARVFGLDRPARWLFAF
                     ALSQVGEFAFVLLAYGVTCGIYSQAFSAPLVAVVALSMALTPIAFL"
     gene            complement(1137..1757)
                     /locus_tag="VFQ61_15750"
     CDS             complement(1137..1757)
                     /locus_tag="VFQ61_15750"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9955962.1"
                     /translation="MAKILLQFAHPILERSRVNAPLLAAARSVEGVLVNDLYERYPKL
                     DIDVAREQELLLKHDVIVFQHPFYWYSAPAIVKEWQDLVLEHGFAYGHTGHALSGKAT
                     FNAITTGGPESAYQRGGYNRFTVRELLAPFDQTAQLCGMRFLAPFVVYGSLRLPTTSS
                     LEPYAERYRRLLQALAHDELDWDRASSIQNLATLFEPLPGTLELQP"
     gene            complement(1902..2882)
                     /locus_tag="VFQ61_15755"
     CDS             complement(1902..2882)
                     /locus_tag="VFQ61_15755"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HET9955963.1"
                     /translation="MSELGIIVSAMVPVLVVTGLGALLRRVGWLREAADGSLLTLAVN
                     VLTPCLIFDKVLASRSLADPRNVLWGPLSGFVISVLSIGASLLAAQLLRRRPVALDSS
                     EAAQVEAQQRSRRSFALATGLQNYGYFPLPLALSLYGDETAAVLFAHNLGVEVALWTV
                     GIAVLARASWRGAAKRLLSAPVLSILCATALNLVLPRDAVPAAVRGSIGMIGAAAVPV
                     GLLLIGATMADHGRQVFGRGSARVVLLACALRLGALPTLYWAARLVLTPTAELSHVFA
                     LQAAMPAAVMPIVLSKHYGGDSLTALRVVFGTTLGSLVTLPLWIHFMSGS"
     gene            complement(3066..7928)
                     /locus_tag="VFQ61_15760"
     CDS             complement(3066..7928)
                     /locus_tag="VFQ61_15760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9955964.1"
                     /translation="MSSVAEQLGVAPVSARELRIKSGIMMVDRLRLSPFWVSSLLLVL
                     NACGGTPDVPIDDQPGKPTGGASSGGATANAGRGGSVIGTSGGRTSIGDAGSTSTDPC
                     TGEDPPPDCFQPSGPACGDGEINQDSEDCDDGNSLPGDGCSGVCAVEPFFECPDPGQP
                     CVSTIACGDGRIDPGEFCDDGNAEDSDGCSADCSTRDPLYVCPEEGKPCRLLYDCGDG
                     RVNGTEECDDGNETDGDGCDAECQKEDGYICRRPGMVCVANEYCGDGKIQTSLGEECD
                     DGDDVRGDQVVWGDGCSGICKLDANYECKTPGEPCTTTIICGNGKREVGEICDDGNTK
                     DGDGCSAQCVQDPAYTCEEGKLCTRTYVCGDGKRNVGETCDDGNAKDGDGCSATCLIE
                     QGYDCRNIGMPCTVKVVCGDGVIQPGEACDDGNTQYFDGCSGICQVDPFYTCPKPGQL
                     CTSTIVCGDGRKDPGEFCDDGNKNSGDGCQANCTVDPNFVCRTPGQPCVRLVECGDGR
                     VNGSETCDDKNTTSNDGCSATCQVESGYRCPTPGVKCETITFCGDGKRQTGEACDDGN
                     KVGNDGCSSQCTVEADWSCTDTNPSRCTYSVACGDGKVGGTEACDDGNKVNGDGCSAT
                     CSQVETGYDCSRPGFPCRSVCGDRLRRGLEQCDDGNTVSGDGCDSTCRIEDNTICTGT
                     ADQVSTCRDAVCGQNGKEGTEPCDDGNAVWGDGCTPDCHVEPTCTGGSACTSSCGDGV
                     KFPNEDCDDGNTLNGDGCSAACKVESGFKCEVDTAVPDLVTLPLVVRDFTAGPPYSPA
                     TADPGITTGHGDFQWPWDTYQQNSVVYGFVAQNLGADKRPAWGAPACTNAINRNGQTY
                     CMPTVQNTTSFSRWFQDVDAYNNTYARSLFLRRTTGNTYVYDSNTHRVDGTAYPTTGT
                     GSGYKGFWPLDDIAGVTKYSQCAISGQGTAPTHNFHFTSETHHWFQFDSAAPPTLTFT
                     GDDDVFVFIAGKLVLELGGVHERVVGSVALNAAGNAVITPARRTDGTAMTTVGTAKMT
                     TSLGLTNGGIYEFAVFQAERNSCESNYRLELQNFNLKKSKCSPVCGDGVVTPGEECDD
                     GSNNTSTPTYGKCTAGTCRLGPSCGDGIKNGSEQCDNGTNNSGWGSTGANACAPGCVT
                     PPRCGDGKVDAGFEACDLGASNSDTGLCTRSCTAGPRCGDGIKNGTEQCDDGLNDGRY
                     GGCTASCQNAPRCGDGVVQGDWGEECDGSNGCDSSCHKLCGNGRVDALETCDDGLNDG
                     RYGGCTSTCQNGPRCGDGVKQAQEQCDNGSANSSTGLYNGCTTACVWGPRCGDGIVNG
                     SETCDDGINDGTYNSCTSDCKPGPRCGDGVVQAAAGEQCDGTPDCASTCVKLGRCGDG
                     RVDSGEECDDGEPPGGRNSSTGEYGKCTTSCVWGPRCGDGVTQGREQCDEGTGKGKND
                     GGYQECNSMCQIEGYCGDGVVNGPETCDDGINDDFYESCTPDCQGFGPRCGDNKVQPE
                     WGEECDDDKDPNCARCRLGALCGDRVVQKGEQCDDGTNDGGYGECGPSCQYGPRCGDG
                     VVQTAHEQCDDGEEENTGGYGKCAPGCVYGPYCGDGKLQKQFEECDDKNNKNSDGCSA
                     ACKIEIVIPR"
     gene            9114..10586
                     /locus_tag="VFQ61_15765"
     CDS             9114..10586
                     /locus_tag="VFQ61_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9955965.1"
                     /translation="MKRLSAIFALTVSMPLSFTFACGGDDDAPSNSGGRSGSSNAPTG
                     GKNTSGGAPHAGASSEPEAGAGNTSTGESGAGGGGQTGSPEMGGSAGDGGSGGATPNE
                     NVIEGRVIDPMGSARAGVSVIVEGRSDLTDAEGYFRFENVGGTYDVLAFPTDGQTAFV
                     YQGITRRNPILIVNAEDRVHTSRVSGMTDYPASAVTSFFGRVGLASHKHAGVSSPLDL
                     KAQTYGPLEISWTGAERYKQDLLALVWIRKENEEPLEYLGFGEAAVDVRADEEDVNAD
                     IHVDDLSEEKTASTRSLSISGQVPEGYQVLRHGLEFGTIPLLDKQTDALSASYNVPSG
                     LKTGHLVFTVESAMLETPEGPAVQRSTAQWVVTDDQEKLDFELPAAPTPVVPLNGAAG
                     VNKSTLFQWQPFTGGIHRLEVSWGESHVVVYTQADSTKLPDLAVVGLAYPSDGEFEWK
                     VSGLGPVESIDEWVASADPLVSLGALHSAESETRAFTISE"
     gene            complement(10720..11442)
                     /locus_tag="VFQ61_15770"
     CDS             complement(10720..11442)
                     /locus_tag="VFQ61_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955966.1"
                     /translation="MSEHEPLEPFPSFRRMLSAAGLGRVSAFDVAWFNDLAPPEERLP
                     DFERGSALAFLIGNGRELWPEFCARLCADSALRCSENPLDEYVVGAVERAVQAWADRT
                     EVLFAHTLEPRPIPIQRIAQAAGFAHLSPSHLSVHAEFGPWFALRAVVVIDRKPENLQ
                     RRPAPDPCSSCRKPCLPLFREAEQKLSETRTVLHTDESLWRAFLAVREACPIGRDARY
                     DEAQIDYHYSKRLDRLLRLLAV"
     gene            complement(11492..12220)
                     /locus_tag="VFQ61_15775"
     CDS             complement(11492..12220)
                     /locus_tag="VFQ61_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HET9955967.1"
                     /translation="MQRRLLSWLTVGLMGSLVAGAAFADPPAAPAATPAPAAPPAAAP
                     AKPLSGPSADEIADRVQAFYDRTKTFRAGFRQRYTVAAYNKVKDSSGSVVFEKPGKMS
                     WRYTNNGNRVVSDGKLIRIYEKENKQMYEQPLEKSQYPAALSFLTGTGSIKQFKLTKL
                     DPKQLGFEGGYVLLGEPREPNAAYQKVFLYVDAGTYQVRRVMLLDAQRNRNRFDFVNP
                     EVNTKAAPGEFTFSPPPGTQVIRP"
     gene            complement(12371..12928)
                     /gene="ruvC"
                     /locus_tag="VFQ61_15780"
     CDS             complement(12371..12928)
                     /gene="ruvC"
                     /locus_tag="VFQ61_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET9955968.1"
                     /translation="MLTIGIDPGTLHLGWGLVLRQGTRLKHVAHGIVHLPPKSPLSER
                     LLGISQALSAILAEHRPAVSAVESLFFHKDPQAAAKLGHARGVVLLCLQQAQVPLFEY
                     APARVKRTIVGNGQAEKRQVALMVRALLGLSEPPAADAADALAIAITHLRVGSLTATL
                     RGGAPETPHPLLQTLRRRKALSRRV"
     gene            complement(13477..13815)
                     /gene="rpsS"
                     /locus_tag="VFQ61_15785"
     CDS             complement(13477..13815)
                     /gene="rpsS"
                     /locus_tag="VFQ61_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET9955969.1"
                     /translation="MARSIKKGPFIDGHLLKKIEDAQAAQSKKVIKTWSRRSTIVPES
                     IGLTFAVHNGRKFVPVFVSENMVGHKLGEFAPTRTFHGHAGDKKAAAGGTGGGAKPGP
                     SKSGGAPGKR"
     gene            complement(13819..14649)
                     /gene="rplB"
                     /locus_tag="VFQ61_15790"
     CDS             complement(13819..14649)
                     /gene="rplB"
                     /locus_tag="VFQ61_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET9955970.1"
                     /translation="MGMRAFKPTSAGRRFYTVADFAEITRDRPEPSLVEPSPSTGGRN
                     HYGRVTSRFRGGGHKQKYRLIDFKRGKIGIPARVAHIEYDPNRTARIALLHYTDGEKT
                     YILAPDGLKQGDTVISSRYADIRPGNCLPLGEIPAGTMVHNVEMRKGKGGQLVRSAGV
                     AAQLMAKDGDYAQLKLPSGEVRKIHVACRATVGQVSNLDHQNIALGKAGRARWLGRRP
                     HNRGVTMNPVDHPMGGGEGRTSGGRHPCSPWGQLAKGLKTRNNKRTDTMIVKHRKAKG
                     "
     gene            complement(14653..14946)
                     /gene="rplW"
                     /locus_tag="VFQ61_15795"
     CDS             complement(14653..14946)
                     /gene="rplW"
                     /locus_tag="VFQ61_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET9955971.1"
                     /translation="MKPEHIIKRPIALTEKASRLKEQNKVVFEVAPQANKIEIRNAVE
                     TLFSVKVTEVNTLIQRGKVKRMGRDEAKRRNWKKAVVTLREGDDIQFFDESKE"
     gene            complement(14943..15578)
                     /gene="rplD"
                     /locus_tag="VFQ61_15800"
     CDS             complement(14943..15578)
                     /gene="rplD"
                     /locus_tag="VFQ61_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET9955972.1"
                     /translation="MAKVDVFNLKREKVGELELSDEVFGAEVKEHLFQEVVVAQLASR
                     RAGTHAAKERAAVSGSTKKIYKQKGTGRARHGPIRAPLFVGGGRAHPPKPQDFSYRPP
                     RRVRVGALRSALSMILKEGRLIVVEDIDLGEIKTKKLAGVLDALQTGNKTLVVDDSDN
                     TNLKLSIRNMPNSQYLPPEGVNVYDLLRHDHLVVSKAAAKALEARCLERKP"
     gene            complement(15593..15904)
                     /gene="rpsJ"
                     /locus_tag="VFQ61_15805"
     CDS             complement(15593..15904)
                     /gene="rpsJ"
                     /locus_tag="VFQ61_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET9955973.1"
                     /translation="MADATKIRIRLKAFDHQLLDKSTTDIVETARRTGARVAGPIPLP
                     TSIRRYTVLRGPHVDKKSREQFEVRTHKRLIDILEPTPQTLDALMKLDLSAGVDVEIK
                     S"
     gene            complement(15919..>16087)
                     /locus_tag="VFQ61_15810"
     CDS             complement(15919..>16087)
                     /locus_tag="VFQ61_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET9955974.1"
                     /translation="GSVQLPEGVKMVMPGDNVTVQVELITSVALEEQMRFAIREGGKT
                     VGAGVVTQILE"
BASE COUNT         2946 a         5207 c         4978 g         2956 t
ORIGIN      
        1 caagaaagcg atgggcgtga gcgccatgct gagcgcgacc acggccacga gcggcgcgct
       61 gaacgcctgg ctgtagatcc cgcaggtgac gccatacgcg agcaacacga aggcaaactc
      121 gcctacttga gacagcgcga atgcaaacag ccaacgagcg ggtcggtcga gcccgaagac
      181 gcgcgccacg ccgtacagca ccagtagctt gagcagcata ctgctcacga cgccaactcc
      241 cacctgccac gggtggtggg cgacgtagcc gaaatcgatc tgagctccaa ccgagatgaa
      301 gaagaggccg agcagcaaac ccttgaatgg ttcgatgtcg gattcgagct cgtgtcgata
      361 ctcactctct gccaacacca cccccgccaa gaaggtgccg agtgcagccg agaggcccac
      421 gtagctcatc agcaatgcaa tcccgaccac cagcatcaga gccgccgccg tgaatgcctc
      481 cctcaaacga gtttcggcca gccagcgaaa cgcggggcgc accaagtaac gaccgcccaa
      541 caccaccagc gctacggccg cgagcaccga tgccgcgcgg acccagcgcg aaggctccgt
      601 ctccgtactc acggtggccg cgccgtcgcc cagcaacggg aacaaagcca aaatgggaat
      661 gaccgcgatg tcctggaaca acagcaccga gaacgcggct tgaccgcccg ctccgtgcga
      721 gagcccgcgt tcgttcaacg actgcaacac aatcgcggtg gacgacatgg agaagatcat
      781 gcccaccgcg agggccggct tgagcggcac gcccagcgca aatgcgaccg cgccgaatac
      841 caagctggtg cacagcacct gcagcccgcc caagcccaag atcggtcgac gcaacgacca
      901 caagagcgaa ggtcgcagct ccagccccac caggaacaac atcatcacca cgccgaattc
      961 ggcgaagtgc atcacgtcgt gcccgtcgtg cccaactagg cccaatgcga acggcccgat
     1021 cgctaccccc gcgagcagat agccgagcac ggagcccaag cccaagcgct tggccagtgg
     1081 cacgccgagt acggcggcga ccaggtaaac gaaagcctgc ttcaaaaagt cgtttgtcat
     1141 ggctgcagtt cgagggttcc cggcagcggc tcgaacagag tcgcgaggtt ttgtatgctg
     1201 gaggcgcggt cccaatcgag ctcgtcgtga gccagcgcct gcagcagccg tcggtaacgc
     1261 tcggcgtacg gctcgagtga gctcgtcgta ggcaagcgca agctgccata caccacgaat
     1321 ggcgcgagga agcgcatgcc gcagagctgc gcagtttgat cgaacggtgc cagcaactcg
     1381 cgcaccgtga agcggttgta tcccccgcgc tggtaggccg attctggccc acccgtggtg
     1441 atggcgttga acgtggcttt gccgctcagc gcgtgcccgg tgtggccgta ggcaaagcca
     1501 tgctcgagca ccaagtcttg ccattctttg acgatggccg gcgccgagta ccagtagaag
     1561 gggtgttgaa acacaatcac gtcgtgtttc aacagcaact cctgttcgcg agccacatcg
     1621 atgtcgagct tcgggtagcg ctcgtaaagg tcgttcacga gcaccccctc cacgctgcgc
     1681 gcggccgcta gcagcggcgc gttgacgcgc gatcgctcga ggatgggatg ggcaaactgc
     1741 agcaagatct tcgccatggc cggtcgagtt tccgtagacc gatggctcat accgtagcgt
     1801 gcccgcgacg tcgaacccgc gcgcaggtgt tcgagcgcag gatgaaccga gagccgagcc
     1861 gaagtctcac tccaagccag tcgcacacgc catgccgggg ttcacgagcc ggacatgaag
     1921 tggatccaaa gcggcaacgt gaccagcgaa cccagcgtgg tcccgaacac gacgcgcaga
     1981 gccgtgagcg agtctccccc ataatgcttg ctcaatacaa tcggcatcac ggccgcgggc
     2041 atggccgcct gcaaggcgaa cacgtgtgag agctccgcgg ttggcgtcag cacgagccgg
     2101 gcggcccaat agagagtcgg cagcgccccg agccgtaaag cacaagccag caacacgacc
     2161 cgcgcgctac cccgcccaaa cacttgtcgt ccgtgatcgg ccatcgtcgc gccgatcagc
     2221 aataatccaa cgggcacggc ggcggccccg atcatgccga tactgccgcg aacggcagcg
     2281 ggcaccgcgt cgcgcggcag caccaagttc agcgccgtcg cgcacagaat ggacagcact
     2341 ggggccgaaa gcaaacgctt ggcggcgccg cgccacgaag cgcgagccaa gacggcgatg
     2401 cccacggtcc agagcgcgac ctcgaccccc aggttatgtg cgaacaacac cgcggcggtc
     2461 tcgtcgccgt acaacgagag cgcgagcggc aacgggaagt atccgtagtt ttgcaggccg
     2521 gtcgcgagcg cgaagctgcg acgtgagcgc tgctgcgcct cgacctgcgc cgcctccgag
     2581 ctatccagcg cgacggggcg gcgtcgaagc aactgcgcgg cgagcagcga agcgccaatg
     2641 ctgagcacgc tgatcacgaa gccagagagc ggcccccaaa gcacgttgcg tggatcggcg
     2701 agggagcggc tagccaacac tttgtcgaaa atcaagcacg gcgtgagcac gttcaccgcg
     2761 agggtgagca ggcttccgtc cgccgcttcg cgcagccagc ccacccggcg cagcagcgcc
     2821 ccgagcccgg tcacgaccag aacgggcacc atggcgctga cgatgatccc gagttcactc
     2881 acgggccggc cttatatcca atacgcgccg actcggccgc aataaagtgg ggctcgactc
     2941 accgcggcac atgccgcgct ccggcgcaac aaaaagcacg atgccgtgag cccgagtatt
     3001 cggagtcacg gcatcgttgg cttccgtctc acagcaccga cacacaccgt cggtgcgcga
     3061 tcgtttcagc gcggaatcac gatctcgatc ttgcaggcgg cgctgcagcc gtcgctgttc
     3121 ttgttgttct tgtcgtcaca ttcctcgaac tgcttctgca gcttgccgtc accgcagtac
     3181 ggcccgtata cgcagcccgg agcgcacttg ccgtagccgc cggtgttctc ttcttcaccg
     3241 tcgtcgcact gttcgtgcgc ggtctgcacg acgccgtcac cgcagcgtgg tccgtactgg
     3301 cagctcgggc cgcactcgcc gtagccgccg tcgttggtgc cgtcgtcgca ctgctcgcct
     3361 ttctgcacga ctcgatctcc gcacagcgca cccaagcggc aacgcgcgca gttgggatcc
     3421 ttgtcgtcat cgcactcctc accccactcc ggttggacct tgttgtctcc gcagcggggg
     3481 ccgaaacctt ggcaatcggg cgtgcagctc tcgtagaagt cgtcgttgat gccgtcgtcg
     3541 cacgtctccg ggccgtttac gacgccgtcg ccgcaatagc cctcgatttg gcacatcgag
     3601 ttgcactctt ggtagccgcc gtcgttcttg cctttgcccg taccctcgtc gcactgctcg
     3661 cggccttgcg tcacgccgtc gccgcagcgg gggccccaca cgcagctcgt ggtgcacttg
     3721 ccgtactcgc cggtgctcga attgcggccg ccaggcggct cgccgtcatc gcactcttca
     3781 ccggagtcga cacggccatc tccgcagcga cccagcttga cgcaggtgct cgcgcaatcc
     3841 ggcgtaccgt cgcattgctc accagcagcg gcttggacca cgccatcacc acagcgcgga
     3901 cccggtttgc agtcgctcgt gcagctgttg tacgtgccgt cgttgatgcc gtcatcgcag
     3961 gtctcggagc cgttgacgat gccatcaccg caacgggggc cccacacgca ggcggtagtg
     4021 cagccgttgt aaaggcccgt gctcgagttg gcggagccgt tgtcgcattg ctcctgcgct
     4081 tgtttgaccc cgtcaccgca gcgcggcccg ttttgacagg tgctggtgca cccgccgtag
     4141 cgaccgtcgt tcaagccgtc gtcgcaggtc tcgagcgcgt cgacgcgacc gttgccgcat
     4201 agcttgtgac aggagctatc gcagccgttg ctgccgtcgc actcctcacc ccagtcacct
     4261 tgcacgacgc cgtcaccgca acgcggcgcg ttttggcagc tcgccgtgca accaccatag
     4321 cggccgtcgt tcagaccatc gtcgcactgc tcggtgccgt tcttgatgcc atcaccacaa
     4381 cgtgggcccg ccgtgcacga tctggtgcac agcccggtat cggagttact ggctccgagg
     4441 tcacacgcct cgaagcctgc atcgacctta ccgtcgccac acctcggagg cgtgacgcaa
     4501 ccgggagcac aagcgttggc gccggtgcta ccccagccgc tgttgttcgt accgttgtcg
     4561 cactgttcgg agccgttctt gatgccgtcg ccgcaggacg ggccgaggcg gcaggtgccc
     4621 gcggtgcact tgccgtaggt tggcgtgctc gtgttgttcg agccgtcgtc acactcctca
     4681 ccaggcgtta cgacgccgtc gccgcacacc gggctgcatt tggacttctt gaggttgaag
     4741 ttttggagct cgaggcggta gttcgactcg cagctgttac gctccgcttg gaacaccgca
     4801 aactcgtaga tcccgccgtt ggtgagcccc aagctggtgg tcatcttggc ggtaccaacc
     4861 gtcgtcatcg cggtgccgtc ggtgcggcga gccggggtga tgacggcgtt gcccgcggcg
     4921 ttcagagcca cgctgcccac cacgcgctcg tgaacacccc cgagctctag tacgagcttg
     4981 ccggcgatga acacgaacac gtcatcgtca ccggtgaacg tcagcgtggg cggcgccgcg
     5041 ctatcgaact ggaaccagtg atgggtttca ctcgtgaagt ggaagttgtg ggtgggcgcc
     5101 gtgccctgac cgctgatcgc gcactggctg tacttggtca cccccgcgat gtcatcgagc
     5161 ggccagaagc ccttgtagcc cgagcccgtg cccgtggtcg ggtatgcggt gccgtccacg
     5221 cggtgcgtgt tgctgtcgta aacgtacgta ttgcccgtcg ttcgccgcag gaacagtgac
     5281 cgcgcatagg tgttgttgta cgcatcgacg tcttggaacc agcggctgaa gctggtcgtg
     5341 ttctgcaccg tcggcataca gtaggtctgc ccgttacgat tgatggcgtt ggtacaagcc
     5401 ggagcccccc aggcgggtcg cttatccgca cccaggttct gggccacgaa gccgtaaacc
     5461 acgctgttct gctgatacgt atcccacggc cactggaagt cgccgtggcc cgtggtgatg
     5521 cccgggtccg ctgtcgcggg ggagtacggt ggccccgccg tgaagtcgcg caccacgagt
     5581 ggcaacgtca ccaaatccgg aaccgccgtg tccacctcgc acttgaagcc tgactcgacc
     5641 ttacaggccg cagaacaacc gtctccgttg agcgtgttgc cgtcgtcgca gtcctcgttc
     5701 gggaacttga cgccgtcgcc gcacgaagaa gtgcaggcgc tccctcccgt acacgtcggc
     5761 tcgacgtgac aatccggcgt gcagccatca ccccacacgg cgttgccgtc gtcacagggc
     5821 tcggtgcctt ccttgccgtt ctggccgcaa accgcgtctc ggcaggtcga gacctggtcc
     5881 gcagtgcccg tgcaaatagt gttgtcttcg atgcgacacg tcgaatcgca gccgtcgccg
     5941 ctcaccgtgt tgccgtcgtc gcactgctcg agtccgcggc gcagccggtc cccgcacacg
     6001 ctccggcagg ggaaacctgg tcgcgagcag tcgtatccgg tctccacctg cgagcaagtg
     6061 gccgagcaac cgtcgccgtt caccttgttg ccgtcgtcgc aggcctcggt gccacccacc
     6121 ttgccgtcgc cgcaagccac tgaataagtg cagcggctcg ggttcgtgtc ggtgcagctc
     6181 caatccgctt cgaccgtgca ctgggatgag cagccatcat tgccgacctt gttgccgtcg
     6241 tcgcaagcct cgcccgtttg acgtttccca tcaccgcaga acgtgatggt ctcgcacttc
     6301 acgcctggcg tcgggcagcg atatccgctc tcgacctgac aggtcgcgga gcaaccatca
     6361 ttgctagtgg tgtttttgtc gtcgcaggtc tcggaaccat tcacgcggcc gtccccgcac
     6421 tcgaccaagc gcacgcaggg ttgacccggg gtgcggcaga cgaagttcgg gtcgacggtg
     6481 cagttggcct ggcaaccgtc accgctgttc ttattgccat cgtcgcagaa ctcacccgga
     6541 tccttgcgac cgtcaccgca gacaatcgtc gaggtgcata gctgtccggg cttgggacag
     6601 gtgtagaaag ggtccacctg gcagatgcca gagcagccgt cgaagtactg agtgttgccg
     6661 tcgtcgcacg cttcgccggg ctggatcact ccgtcgccgc acacgacctt gaccgtgcac
     6721 ggcatcccga tgttgcggca gtcgtagcct tgctcgatca aacaggtggc gctacagcca
     6781 tctccatcct tggcattgcc gtcatcgcag gtctcaccta cgttgcgctt gccgtccccg
     6841 cacacgtaag tccgcgtaca aagcttgcct tcttcgcagg tgtaagctgg gtcttgcacg
     6901 cattgcgcac tgcaaccgtc accgtctttg gtgttgccgt cgtcacaaat ctcgcccacc
     6961 tcccgcttgc cgttgccgca gatgatggtg gtcgtgcaag gctcgcccgg agttttgcac
     7021 tcgtagttcg cgtctagctt acagatgccg gagcaaccat cgccccagac cacctggtca
     7081 ccgcgaacgt cgtcgccgtc atcgcactcc tcgccgagtg acgtctgaat cttgccgtcg
     7141 ccgcagtatt cgttggcgac gcagaccatg cccgggcgcc tgcagatgta gccgtcttct
     7201 ttttggcatt cggcatcgca accgtcgcca tcggtctcgt tgccgtcgtc gcattcctcg
     7261 gtgccgttta cgcgcccatc accgcagtca tatagaagac gacaaggctt gccttcctcc
     7321 gggcacacgt aaagcgggtc gcgcgtcgag cagtcggcgc tgcatccatc actgtcctcg
     7381 gcgttcccgt cgtcgcagaa ctcacccgga tcgatacgcc cgtcgccgca cgcgatcgtc
     7441 gagacacaag gctggcccgg atcggggcat tcgaagaacg gctctaccgc gcacacgccc
     7501 gaacagccgt cccccggcag gctgttgccg tcgtcacagt cctctgaatc ttggttgatc
     7561 tccccgtcgc cacacgcggg accagaaggt tgaaagcagt ctggtggcgg gtcttcaccc
     7621 gtacacggat cggtgctcgt cgagccggca tcgccgatac tggtacggcc acccgaggtg
     7681 ccgatgacgc tgccgcctcg ccccgcgttg gcggtagctc cgccggaact agctcccccc
     7741 gtgggtttgc ccggctggtc atcgatcgga acatccggtg ttccgccgca agcgttcagc
     7801 acgagaagca gagaagatac ccagaatgga cttaggcgta atctatcaac catcataatc
     7861 cccgacttga tacgaagttc ccgcgcggaa accggcgcca cgccgagctg ctcagcgacg
     7921 ctggacacgg ctccttgcta tcagccgaat tggagaggcg gtgtggctcg aaggaatggt
     7981 gcaggacgaa aatcgattcc agctgtacac gctgcaatgc cttacccgct gcgcggctag
     8041 aatctcggac atcatgagct caaaccgtcc gtacgatgca gcgctcgagg tcgaatgtca
     8101 gatgagcaga cctcatattt acagctcatg attgcagctg atgtcctcag ctgatctctt
     8161 cggcgaggcg gcgaagcgcg gcaagcgtca catcttacag attttgagac tttgccggga
     8221 aacttttacg cccgataagt gagagaggcg tgcttgccgt ctacgctttt tcctccgagt
     8281 tcccgcccag cgttgccggg ccgcggcatg acgcgcgttc gcatcccgtg cgcggaacag
     8341 ctggctctgg ttccccctgg gtagcccttc gatacgagtg cggcagcagg gtcatcgggc
     8401 tccgaagaag ccatgtgcct gagcacgttc gacaggcgtg ctgatacgca caccccaaag
     8461 ctccgacgga gtttggaccg acctccggga gagcaaacag aatcattgga acagggccag
     8521 agcaagcagc gcccgggaaa gtcgctggcg tgtgtcgcgc gttgccccaa ggcgtgaata
     8581 cacagccatt cagcaagtta gactgcgccg cgccgtgcac cagacggcgc ccccaaaacc
     8641 aagaggagta cgtgagaaag gtcacgccga acggcccgga gaaggtcgtc aaacgtgggc
     8701 acaacgagca cacgcggcgc cagctccccg gctgcaggag caacccaagc cgacgctcga
     8761 gacaacatgg ttgccgctcg aacgcgctga aacgtcgaga tcactggcga gcagacgcga
     8821 acgtttttca agaagcgaac ttcacctggc cgcgtccaga gcatctccgg aagctttgag
     8881 ccgcgccaat tcacgcgcgc agcaggcgat gtggaggaag agcactgctc gcggcgctcc
     8941 acatgctatc ttcgcccatt ttatttcgtc ttggcctggg ctagcctccc cgcacttcga
     9001 cgcctgggat atcccggagg ccagcctgag gtggcctggc ctccaactcg ggcggacaag
     9061 gggtcgttta tcaaagtagt gctgccgaga gggcagtcag ggagtttgcg ctaatgaaga
     9121 ggctttcagc catctttgcc ttaaccgtct cgatgccgct gagtttcacg ttcgcctgcg
     9181 gaggcgacga cgacgcgcca tcgaactctg gcggtcgcag cggttcctcg aatgcaccga
     9241 cggggggtaa gaacacgagc ggcggtgctc cgcatgcggg cgcgagttca gagccggagg
     9301 ccggagctgg aaatacctcg acgggagaaa gcggcgccgg cggcggtggc caaaccggct
     9361 cgcccgagat gggcggcagc gccggagacg gtggcagcgg aggcgcgact ccgaacgaaa
     9421 atgtgatcga agggcgcgtc atcgatccga tgggtagcgc gcgtgccggc gtgtccgtca
     9481 tcgtcgaggg tcgctccgac ctgacggacg ccgaaggtta cttccggttc gaaaacgtcg
     9541 gcggaactta cgacgtcctc gccttcccaa cggatggcca gacggccttc gtgtaccaag
     9601 gcatcactcg ccggaatccg attctgatcg tgaacgccga agaccgcgtg cacacgagcc
     9661 gcgtctccgg aatgacggat tatcccgcga gcgccgtgac ctctttcttc ggccgagttg
     9721 gactggcgtc ccacaagcac gccggcgtct cctctcctct ggatctcaag gcccagacct
     9781 atggcccgct tgagatcagc tggacgggcg ccgagcgcta caagcaagat ctgctggcgc
     9841 tagtgtggat ccgcaaagaa aacgaggaac cgctcgagta tttgggcttt ggcgaagccg
     9901 cggtcgacgt tcgagcggac gaagaagacg tcaacgcgga tatccacgtc gatgatctct
     9961 cggaagagaa gaccgcgagc acgcggtcgc tctcgatctc cgggcaagtt ccggagggtt
    10021 atcaagtgct ccggcacggg ctggagttcg gcaccatccc gctgctcgac aagcagacgg
    10081 atgcgctctc ggcctcgtac aatgtaccgt cggggctcaa gactgggcac ctggttttca
    10141 cggtcgagag tgcgatgttg gagacgccgg agggccccgc cgtgcaacgt tcgacggctc
    10201 aatgggtagt caccgatgat caagaaaagc tcgatttcga gctcccggcc gcgcccactc
    10261 cggtcgtccc attgaacggg gcggcgggcg tcaacaagtc gaccttgttc cagtggcagc
    10321 cgttcacggg cggcattcac cggctcgagg tgagctgggg cgagtctcac gtcgtcgtct
    10381 acacgcaggc agacagcacg aagctgccgg atttggcggt cgtcgggctc gcttacccca
    10441 gtgacggaga attcgagtgg aaagtgtctg gcttggggcc ggtcgagagc atcgacgagt
    10501 gggtggcgtc cgcggacccg ctggtttcgc tcggtgcatt gcatagcgcc gagtccgaga
    10561 cgcgcgcgtt cactatcagc gaatagtcaa tcgttcgatt cggcttacga cgcccgacca
    10621 gaacgcctga gcttttgcag tcgcgaagct caggcgtttt gcttttggcg cggcccgcgc
    10681 gccgcgatca tctatctttc attcgattcg aggccgagct tagaccgcga gcaagcgcaa
    10741 gaggcggtcc agtcgcttgc tgtagtgata gtcgatttgc gcctcgtcat aacgcgcgtc
    10801 acgcccgatc gggcacgctt cgcgtaccgc cagaaacgca cgccataggc tttcatccgt
    10861 atggagcacc gttcgggttt cgctcagctt ctgctccgcc tcccgaaaaa gcggcaggca
    10921 aggcttacgg cacgacgagc aaggatcggg cgcgggccga cgctgcaggt tctccggctt
    10981 tcgatcgatc accaccaccg cacgtagcgc aaaccaaggc ccaaactccg cgtggacact
    11041 gaggtgactc ggcgagagat gcgcaaaacc cgcggcctga gcaatgcgtt ggatcgggat
    11101 ggggcgcggc tcgagggtgt gcgcgaaaag aacctcggtg cggtccgccc aggcctggac
    11161 cgctcgctcc accgccccca cgacgtactc gtccagaggg ttctcgctgc atcgaagcgc
    11221 tgagtctgcg cacaatcgag cgcaaaactc aggccacagc tcgcgtccgt tcccgatcaa
    11281 aaaggccagc gcgctgcctc gctcgaaatc gggtaaccgt tcttcgggcg gggcgagatc
    11341 gttgaaccaa gctacatcga aggcggagac tcgccccagc ccggccgcgc tcaacatacg
    11401 ccggaaagac gggaacggct ccagaggttc gtgctcgctc acttacgaaa ggctgccccg
    11461 cccgttgctc tggcgctacg acgcacagcg ctcaggggcg aataacctgc gttcccggag
    11521 gcggagagaa ggtgaactcc cctggggcgg ccttggtgtt cacttcggga ttcacgaaat
    11581 cgaagcgatt gcgatttcgt tgtgcatcca acaacatcac gcgacgcact tggtaggtgc
    11641 ccgcgtccac gtataagaac accttctggt aagccgcatt cggctctctc ggctcgccga
    11701 gcagcacgta tcctccctcg aagccgagct gcttgggatc gagcttggtg agcttgaact
    11761 gcttgatgct accggtgccg gtcaggaacg agagcgccgc cgggtattgg ctcttctcga
    11821 gcggctgctc gtacatctgc ttgttctctt tctcgtagat tcgaatgagc tttccgtccg
    11881 agacgacgcg gttgccgttg ttcgtataac gccagctcat cttgccgggc ttctcgaaca
    11941 ccacggaccc cgagctatct ttcaccttgt tgtacgctgc aacggtgtac cgttgtcgaa
    12001 agccagcgcg gaaggtcttg gtacggtcgt aaaacgcctg gacgcgatcg gcgatctcat
    12061 cagcgctcgg gccgctcagc ggtttcgccg gtgccgccgc gggcggagca gcgggagcgg
    12121 gtgtcgcggc gggagcggca ggcggatctg cgaaggctgc ccccgcaacc agcgatccca
    12181 tcaagccaac ggtgagccaa gaaagaagtc ggcgttgcat gacagccttt atacgtacga
    12241 gcccgtgaaa tcctttcacg atccgacggg tcgctgggaa gtagcacgga cgccgacgtt
    12301 tgccgagtca cccagaccgc ggccacgcct aagctaattg cttgcttttc ttgagattag
    12361 tatcgttgcg tcagactcgc cggctcagag ctttgcgtcg acggagcgtc tgtaacagtg
    12421 ggtggggggt ttcgggggca ccgccgcgca gcgtggcggt taagctgcca acgcgcaggt
    12481 gggtgatcgc gatcgccagt gcgtcagcgg cgtcggcggc cgggggctcg gacaagccga
    12541 ggagggctcg caccatcaat gccacctgac gtttctcggc ttggccattg ccaacgatcg
    12601 tgcgcttcac gcgagccggc gcgtactcga acaatggcac ttgcgcttgt tgcaggcaca
    12661 gcaacacgac gcctcgcgca tgccccagct tggcggcggc ctgggggtct ttatggaaaa
    12721 acagcgactc gacggcggag acggcaggac ggtgttccgc gagaatcgcc gaaagagcct
    12781 gcgagatccc gagcagtcgc tccgaaagcg ggctcttggg tggcaggtgc acgatgccgt
    12841 gcgcgacgtg cttgaggcgc gtcccctgac gcagcacgag gccccaacct agatgcagcg
    12901 tcccaggatc gatgccaatc gtcagcacgg cattctgcta gcaggggccc cgcgcgatgg
    12961 aaaggccgaa cgaccgtaga tgacggattt acgtgaagcg cttatgctcg acccaggcaa
    13021 ggccgacggc tgattgcggc aacggctcaa gcgcgacgcg agaacaagct gcggaaccag
    13081 gactccgagt ttcgcgcttc ggcttcaccg ggcggggcct gcggagcggg gccacgcgag
    13141 tcgctctccc ggcactgaca acgctgttct ttgggcacac ccccaagaac ctgctccacg
    13201 tgcataccgc agcccgcgaa ggtgggacgt ccgcatttgg ggcactcgac tcggcggcac
    13261 atcagcgttg ctccgtttgc aaactaggat tggacggggc ccaggtgtca gttcgaagga
    13321 ccttgccgag gttccccttg gttatgtgta gcgtcatacc cggtgagagc cgtgccaggg
    13381 aaaaaggatt catccaaatc gacgaagtac aaaagggctc ggccccacct tgcgatgcaa
    13441 ggcagggccg aattcaccag gctgctgacg actagttcag cgcttgccgg gcgcgccgcc
    13501 ggacttggac ggaccgggct tggcgccgcc gccggtacca ccggcagctg ccttcttgtc
    13561 gcccgcgtga ccgtggaagg tccgggtggg cgcgaattca ccgagcttgt gtccgaccat
    13621 gttctccgac acgaacaccg gcacgaactt gcggccgttg tgcaccgcga acgtgaggcc
    13681 gatcgactcg ggaacgatgg tggaacgccg cgaccaggtc ttgatgacct tcttggactg
    13741 cgcggcttga gcgtcttcga tcttcttcag gagatgcccg tcgatgaacg gacccttctt
    13801 gatactacga gccatggatc agcccttcgc cttacgatgc ttgacgatca tcgtatcggt
    13861 gcgcttgttg tttcgagttt tgaggccctt cgcgagctga ccccaaggtg agcacgggtg
    13921 gcgaccaccg ctcgtgcggc cttcaccacc gcccatcgga tggtcgacgg ggttcatggt
    13981 cacgccgcgg ttgtgcggac gacgaccgag ccagcgagcg cgaccggcct tgccgagcgc
    14041 gatgttctgg tgatcgaggt tcgagacctg gcccaccgtg gcgcggcaag cgacgtggat
    14101 cttacgaacc tcgccgctcg ggagcttcag ctgggcgtag tcaccgtcct tggccatgag
    14161 ctgggccgca acgccagcgg agcgcacgag ctgaccgccc ttgcccttcc gcatttcgac
    14221 gttgtgcacc atcgtgcccg ccggaatctc accgagcggc aagcaattgc cggggcggat
    14281 gtcggcgtaa cggctggaga tgaccgtatc tccctgcttc agaccatcgg gcgccaagat
    14341 gtaggtcttc tcgccgtcgg tgtagtgcag gagcgcgatg cgagcggtcc ggttgggatc
    14401 gtactcgatg tgtgcgaccc gcgccggaat gccaatcttg ccgcgcttga agtcgatcaa
    14461 gcggtacttt tgcttgtggc caccgccgcg gaaacggctg gtgacgcggc cgtagtggtt
    14521 gcgtccgccc gtgctggggc tgggttcgac caacgacggc tccggccgat cgcgcgtaat
    14581 ctcagcgaaa tcggcgactg tgtagaagcg ccgaccggca gaggtcggct tgaatgcgcg
    14641 catgcccatc gctcactcct tggattcgtc gaagaattgg atgtcgtcac cttcacgcag
    14701 cgtgacgacc gccttcttcc agttgcgccg cttggcctcg tcgcgcccca tccgcttcac
    14761 cttgccgcgc tgaatcagcg tgttgacttc ggtgaccttg acggagaaca acgtctcgac
    14821 cgcgttgcgg atttcaatct tgttcgcctg aggagcgacc tcgaaaacca ccttgttctg
    14881 ctctttgagc cgggaggctt tctcggtcag cgcgatcggg cgcttgatga tgtgctcagg
    14941 tttcatggct tgcgctccag gcagcgcgct tcgagagcct tggcggccgc tttagacacc
    15001 acgaggtggt cgtgccggag aaggtcgtaa acgttcacgc cttcgggggg caggtactgg
    15061 ctgttgggca tgttgcggat cgacaacttc aggttcgtgt tgtcgctatc atcgaccacc
    15121 agcgtcttgt tgccggtctg gagagcgtcg agcacgcccg ccagcttctt ggtcttgatc
    15181 tcgccgagat cgatgtcctc gaccacgatc aggcggcctt ccttgaggat catgctcagg
    15241 gccgagcgca gagctccaac gcgcacgcga cggggcgggc ggtagctgaa gtcctgcggc
    15301 ttgggcgggt gcgcacggcc accgcccacg aacaacgggg cgcggattgg accgtggcgg
    15361 gcccgaccgg tgcccttctg cttgtagatc ttcttggtgg agccggacac ggccgcacgc
    15421 tctttcgcag cgtgcgtacc agcgcgccgc gaagcgagct gagccaccac gacttcttgg
    15481 aacaggtgct ctttgacctc ggcgccgaaa acctcgtcgg agagctcgag ctcaccgact
    15541 ttttcccgct tgaggttgaa tacgtcgact tttgccatac tgaggtcctc gctcagctct
    15601 tgatctcgac gtccacgccc gccgacaggt ccagcttcat gagcgcgtcg agggtttgag
    15661 gcgtcggctc caggatgtcg atgaggcgct tgtgggtccg cacctcgaac tgctcgcgcg
    15721 actttttatc gacgtgcggg ccgcgcaaca cagtgtagcg ccgaatagat gtgggcagcg
    15781 ggatgggccc cgccacccgg gcgcccgtgc ggcgcgcggt ttccacgatg tcagtcgtgg
    15841 atttgtcgag cagttggtga tcgaacgcct tcaggcgaat tcgaatcttg gtcgcgtcag
    15901 ccatctgttg aaacgtcctt actcgaggat ctgggtaacg acgccggcgc cgacggtctt
    15961 gccgccttcg cggatcgcga agcgcatctg ctcttcgagc gccaccgacg tgatcagctc
    16021 cacctgaacc gtcacgttgt cacccggcat taccatcttc acgccttcgg gtagctgcac
    16081 gctcccc
//
LOCUS       DASTKU010000460        18199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1649228, whole genome shotgun sequence.
ACCESSION   DASTKU010000460 DASTKU010000000
VERSION     DASTKU010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 18199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18199
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649228"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VFQ61_15815"
     CDS             <1..761
                     /locus_tag="VFQ61_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955975.1"
                     /translation="QWDATAHAYTDVLGESACCADGVRDSCGQLSCYFVPSGARIAVN
                     VELEAQMTPGAAQYEYRSRDTGSDVWSDWDFQLSRTVPFDALADEYCYEVEARSLVTG
                     EVTRVEAGCAAGRDLALPNSEEDLAAAREKEKDPFIQCTVPPEGFEEVWCKTFEEARK
                     TGVCPSWNERACEAAIEVCPPITVVDTEEGDASEFPKAPGDVLPGGSGSSATAGGGCS
                     LTHPASGGTGLELLMMTALGVMIAGRRKKRGSAR"
     gene            complement(824..2911)
                     /locus_tag="VFQ61_15820"
     CDS             complement(824..2911)
                     /locus_tag="VFQ61_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955976.1"
                     /translation="MSREDLRVLTPDALAALANRGLVKRAQKELDAGLAPTLEELPDG
                     TVIGRFGDVETRLPQRTGLSDAPCNCGAAGICRHKLIVVLAYARVAAASADGKSPDAN
                     SPDTQARAPERIEAKSTDAETTSWSPGDFSDEELEHFLGARAWERARALFRKGYVAEV
                     RRAMGPDHAPTALLPTCSVRFLVPRDLNYAHSDSGERAAEAVALAVWAFRAADVRDSH
                     AKRLELEVRDALAENSTIADELSPALALAQELLLEGIIHSRNSMAGQFERVRQTLTAV
                     RLIWPALILEDLEQELSAYHTRSARYSPASAAALISELHARARAVRRPNVLPAARILG
                     TEVASETPLAHLRLSSLGARVRGDASQRLAEVVLADPDTATLLVMRKEYEIKAGQRAP
                     LPSELGRKPFAPRTTLAAIATGQVVTASARRAANRLLVLGQGRVARTSVMPSNGDWSG
                     LPEALRVRNTNQLAQELAERPPTLLRARVLADGVYVLEIAAVEGLSYAPGAQRLSARI
                     VPHAGPSVRLVRSYDVAAPGALDELALALSAKTGPPRFVSGSVRHTASGLELEPIGIV
                     TDRFHVPDLADPSSDPRLPIPGGVATGPNELANPISPITTALSLAANVLDDGVHHGLR
                     RASPNFGGQLGERAAELRRTGLIGLAARFEALKYALAESQARGDTDSETTLTARWADA
                     AIRLRLCEETGQS"
     gene            complement(2908..4038)
                     /locus_tag="VFQ61_15825"
     CDS             complement(2908..4038)
                     /locus_tag="VFQ61_15825"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9955977.1"
                     /translation="MADPKESELLERWRLVLGAPAGQAMPNLSPRGQAMDAALEWLYG
                     RDPELAERGVQRTGGSGESQLTTPDWVNEVHRLFPKETIERLERDAVERFGIDEVVTN
                     LEVLERIEPSESLLRAVLHTKHLMNPEVLAKARALVAKVVEELMRRLSKEVRASFSGV
                     RDRRRPSLHKSAKNFDLRRTIQTNLKHYQPELQRIALEKPRFFSRTRRFTERWQIILV
                     VDQSGSMVGSVIHSAVTAACLWGLPGMRTHLVAFDTQVVDLTADVADPVDLLMKVQLG
                     GGTDIAKAVEYAASLIEAPRRAIVVLISDFYEGGDAHRLVRATSALCEQGSKVLGLAA
                     LDPDANPVYDRALAQELVNVGASVGAMTPGALASWIAEQVQR"
     gene            complement(4031..6367)
                     /locus_tag="VFQ61_15830"
     CDS             complement(4031..6367)
                     /locus_tag="VFQ61_15830"
                     /inference="COORDINATES: protein motif:HMM:NF040143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HET9955978.1"
                     /translation="MTLTVIGVRHHSPACARLVEYRLRQLRPRFVLIEGPADMNARMS
                     ELQLRHQLPLAIFSFYQSEGRYHASWSPFCEYSPEWVALSTAREIGAEVRFMDLPAWS
                     EAFDGVHNRYADDERRYEQALASLCDQFGAEGMDALWDHLFEQPLELEALSSRLQAYF
                     AGMREVSQTMTRDALREAYMARCIAWSIRQGGDVVAVCGGFHKPELERLHSEADGSVW
                     PEFPAAEEDAKHGSYLVPYSFKRLDSFVGYESGMPSPAYYQAVWECGPEHAAQAMLEQ
                     ATRRLRKKKQAISTADLIGASAMALGLARLRGHAALSRCDLLDGFASAVLKSAQTAVF
                     PWNQRGKLPPGTDPILVEVVAALSGERSGRLAPETPRPPLLVAARAELTELGLWPEGP
                     PRKVRLKLTEPVDLIKSRALHRLRVLAIPGLERVSGPTYGTDPVLEEEWSLRGAFEAE
                     SALIEASAYGATLDTAAVARLEERLGGAPDVLEVSALLAEATFVGIERLSSQLLTTLG
                     PLVSREPDFGKLGKAQARLLALYRHDTLLGSQSSPALGAVLEAAFARGLWLFEGLTGP
                     TAPADTTLIHGVMALRDSVRFLRDRANLEASSAAAMMHRKSADAATPPALRGAALGYL
                     WSLHAFDDLTTAEALASSALRRAALPATLGDFLAGLFALAREEIVTAGESALLATLDE
                     LLADMALEEFLVALPALRMAHAYFPPRERERIALRVLALHGQGTAHARDFLKLSAGAD
                     TLAAARSLEARVDELERRFGLEPSTESTLQTTPAEVHG"
     gene            complement(6376..7500)
                     /locus_tag="VFQ61_15835"
     CDS             complement(6376..7500)
                     /locus_tag="VFQ61_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9955979.1"
                     /translation="MSKTKPTPSPSDPVRKVQRPPAEELYAAELEQLRARDDQPNPPG
                     WQLSLQAARRFVMGDPDAGIKRKFVGQPTLVDRALVTLATNRGLMLVGEPGTAKSLLS
                     ELLAAAVSGSSTLTIQGGASVTEDHIKYSWNYALLVAEGPTPRALVPAPIYRGMSEGK
                     LVRFEEITRCPLEVQDSLLSMLSDRVLAVPELPGEAGMVFAREGFNVIATANTRDRGV
                     NEMSAALKRRFNFETVFPIPDYATELELVRSESARLLRESGVPHALTPDVLEVLVTTF
                     RELRSGETKDGQAMERLSSVMSTAEAVSTAHAVGVRGFYLHGSASTPADVVECLAGTA
                     AKDNADDLKRIRRYLEQRAKSKKGEHWRAFYEARKLLPGD"
     gene            complement(7551..11312)
                     /locus_tag="VFQ61_15840"
     CDS             complement(7551..11312)
                     /locus_tag="VFQ61_15840"
                     /inference="COORDINATES: protein motif:HMM:NF024958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HET9955980.1"
                     /translation="MRRLEFSEGSSSKFWQVEQKGGSLLIQWGKIGTNGQAQTKEFAD
                     ETKAKSEADKLIAEKKKKGYIEVNGAATSGVTAMGAPEAPLPAPTANKVKGKAQGHDE
                     GKDAEGKGAEGKGAEGKGATAVSSPTAAAEATPALEETRVPEQGTTSSEAFNPEPVVV
                     LKDEQRAERDAWRELPDATGVKVPELLSSLRDFIVKQRKADKAWIESKSHADAHGKAV
                     ADYALESYAQGILPKEPDAALDGAFAALMRTTYSMRLFAASRGLEYALDAFVESFANE
                     WQHQHNSKGISPEWVVRCDPATNWTPFAGSGEKRVESLAGMLAAADESTRSRAHAHAE
                     QLRKSAALPARVGLALMFADTRWAAEDALEVIALKDQDVYGARDLLYLLRDRRLLDVF
                     PTPEYGRHPVSLLVNAGLECVGVLIKWLDNQYSREDAARALACVESVESGRALALVLD
                     KKDAAPHARALFERRPDLGLIVLGQVVASGGKLSPYAEPLLKGLLRDHPELPARLGSA
                     LDGRTRACIAQLQAVRKRAPAAEADIPRVLVSPPWLAQRTSQALPTLQLEVASPYVPP
                     DWSKQRRAQRVQSMDPYSYTRKQAKIPDKTKPGTPEMDKLVKAALANGNNYYADKIAH
                     LASDEFLLAGLEDGSLPHMPADYLFARFGESIWPRCVASLADNAQEVLSTFSKVQEPR
                     LVRAVAFALDKKTLRKDARHWLEMNAELAVLGLLPLAFGADKHERAAAQKALRVIASG
                     HADAVNRVAQRYGEATVAAVKQLLALPADEYPTKLPTTPKFFELETLPPLSLKNGKGD
                     LPESAIKHLITMLQLSPLDAPLSILAEVVAALEPEAAADFAWQLMSAWLSAGAPSKDV
                     WAFWAVGHLGNDESARRLTPMIRAWPGESAHARAVVGLDVLAAIGTDVALMHLHGIAQ
                     KLKFKGLQEKAREKIDVVAEARGLTAFELADRLVPDLGLDENTGTLTLDYGPRQFVVG
                     FDEQLKPFVRDATGKRLGDPPKPGKADDAEKASAALEQYKTLKKDAKAIAQSQILRLE
                     LIMCAGRRLSAEAFRSFFVAHPLMIHLVRRVLWGVYAEDGKLTSTFRVAEDRTFADSE
                     DDTFELPPHATLGVVHRLELDAATCAKWGQVFGDYEILQPFEQLGRSVFHPTEEELKG
                     DVLKRLTGVTVKTGKILGLDARGWHRGVPQDAGWVWDMYKPLGEFRAELELKGGFCVG
                     YMEGTPPEQELGDLKLHLESGSERAKALFSEVSPSVFSELVRDLEALRD"
     gene            complement(11485..12735)
                     /locus_tag="VFQ61_15845"
     CDS             complement(11485..12735)
                     /locus_tag="VFQ61_15845"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HET9955981.1"
                     /translation="MRPRRDGTRSETSSVNIWRVPTRTRQSTGIRSAELVEHGGLQAF
                     DVRLRRMHAPRFSLTGLTGRLTSAICGLSLVCTLGCSGEDASEQVAANTWKQVLPGGD
                     TACADGKDFSFWTYLGDPGKVVLYLQGGGACFDAVTCAFTATQTVYDWTISEEDYPAQ
                     QKGIFDFARADNPFLGYSFVYVPYCTGDVHLGNISQEYSETLTVEHRGMVNGTAALET
                     LSNEFASASQVVVVGASAGAVASPIYGGLVSDRLPNARVTVFADGSGAYPDDPRVNAE
                     IFENKWGSFASRPQWEENEGLEAQDWGIPRFWVQAGLHAPNMTMARFDYASDRVQGFF
                     MTLLGQDTSDLLASMMENENNIEEAGIVQHSFTSSGSAHTIVEDDKFYDLQVAGVRLS
                     TWLNELVTGHEVSDVLCEDCSTDP"
     gene            12836..13396
                     /locus_tag="VFQ61_15850"
     CDS             12836..13396
                     /locus_tag="VFQ61_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9955982.1"
                     /translation="MADSARRRANYDDLRDVPPHLIAEILQGELVTSPRPGPPHAQAA
                     SSLAGELYAPFHRGRGGPGGWIILNEPELHLRGDILVPDIAGWRRERMPALPVSAAFE
                     LAPDWICEVLSASTASTDRAVKVPIYAREKIGHIWLVDPVLQILEVLRLDGPGYRIAG
                     VWRGDVTVQCEPFETLPIRLLDLWSV"
     gene            13648..14487
                     /locus_tag="VFQ61_15855"
     CDS             13648..14487
                     /locus_tag="VFQ61_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955983.1"
                     /translation="MTNWEDSCRQRLASHLDENGDLEPPWARFPDYERYCMGWRMGSG
                     EDWLGLWHVFVEQLPRDEASRLAYLRRHPPAPINWADHVENVLRLSPLDDADEDEEPP
                     DEPTPARLEELEQLGLIGSDVAFKTWLERQRSEISWPWRHSETPETAARYSTRPLWFW
                     SRQIAQLRPTLALPELPPAWRICEQPLLTGSLASIDPNRGLQSLALLSCAGNVRPPWQ
                     FGLSLSDFRDSFDDDMPYVDAFRLWAISCMDDIAQLTQLCEQLDAPPAWKSWLLEQCG
                     LTS"
     gene            complement(14902..15684)
                     /locus_tag="VFQ61_15860"
     CDS             complement(14902..15684)
                     /locus_tag="VFQ61_15860"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HET9955984.1"
                     /translation="MPKPAVPWSEYDLPPQPVRRGGSGWLGWVLFVAVSAGVGAFVYR
                     GYLPLRRERGALQAQVDEQKQREQRSKKELRETAAQLAELKQSHADLSGKLVHTQAER
                     DKIESELKRIQNELTAKLEPEIQAGNVRIRRRGNDLVVDLSDKILFESGKPEVHENGQ
                     KVLGEVGPTLAQLKDYTLQVGGHTDSTRISSPTTQERFPTNWELSTARATNVVRFLEE
                     TGKLPGERLVAMGFAQYRPAASNATAAGRQQNRRIELVLARR"
     gene            15975..16391
                     /locus_tag="VFQ61_15865"
     CDS             15975..16391
                     /locus_tag="VFQ61_15865"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9955985.1"
                     /translation="MKITVETNVKAPVDHVWRAYTTSADIKQWNAASDDWHTTAATAD
                     LREGGEFSFRMEAKDGSMGFDFAGTYTRVVKNERIEYAFGDRTAQVEFQPSKDGVLVR
                     VTFDAESTHSEEQQRFGWQAILDNFKRHVEAASKLQ"
     gene            complement(17386..17904)
                     /locus_tag="VFQ61_15870"
     CDS             complement(17386..17904)
                     /locus_tag="VFQ61_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955986.1"
                     /translation="MLEVSWAESEYPQLFSALRAVGWWEGRQVEPPAEELAPCLVAND
                     YADEFLRSFSGLSLWRRKVEPNGIAFSYGMNLGFDEKLMRMDPCATADAICKLAGSSF
                     VYPIFDAGTGVAFALEDGKTLFIADTFQGYAWTEDPFAMMDWLYFDVPRPGLALIYLP
                     KDHRPPDFYDFD"
BASE COUNT         3325 a         5991 c         5513 g         3370 t
ORIGIN      
        1 cccaatggga cgccaccgcg cacgcataca cggatgttct gggagaatcc gcgtgctgcg
       61 cagatggagt gagagacagc tgcggacaac tcagttgcta cttcgtcccg agcggggcgc
      121 gcatcgcggt caacgtcgaa ctcgaggcgc agatgactcc gggggcggct cagtatgaat
      181 atcgatcccg ggacacgggg agcgatgttt ggagcgactg ggattttcag ctctccagaa
      241 cagtgccctt cgatgcgctt gcggacgaat actgttatga ggtcgaagcg cgaagcctcg
      301 ttacgggtga ggtcacgcgg gtagaggcag gatgcgcagc tggccgggat ctcgcgctgc
      361 ccaactcgga agaagatctc gcggcagcac gggagaagga aaaggatccg ttcatccagt
      421 gtacggttcc gcctgaaggg ttcgaagagg tctggtgcaa gaccttcgag gaagctcgca
      481 aaacgggagt ttgcccctcg tggaacgaga gggcgtgcga agccgcaatc gaagtttgcc
      541 cgccaattac cgtcgtggac accgaagaag gcgatgcatc cgaattcccc aaagcacctg
      601 gagatgttct gccgggtgga agcggctctt ctgccaccgc gggagggggc tgctcgctca
      661 cgcatccggc gagcggtggc accggtctcg agctactcat gatgacggca ctcggcgtga
      721 tgatcgcggg tcggcgcaaa aagaggggct cggcacgttg agtgaaacaa gagcggtgga
      781 gcggtgatga aactccccag ctcataagcg acctcaactg cggctagctc tgcccagttt
      841 cctcgcacag acgcaatcgg atggcggcgt cagcccagcg cgccgtcaac gtcgtctccg
      901 aatcggtgtc gccacgggct tggctctcgg ccaaggcgta tttcagcgcc tcgaaccgcg
      961 ccgcgaggcc gatgaggccc gtgcggcgca gttcggcagc tcgctccccg agttgcccgc
     1021 cgaagttcgg gctcgcgcgc ctgagtccgt ggtggacgcc gtcatccagg acgtttgctg
     1081 cgagcgagag tgccgtcgtg atggggctga ttgggtttgc gagctcgttc ggcccggtcg
     1141 caacccctcc ggggatgggc aaccgagggt cgctgcttgg gtcggctaag tcgggcacgt
     1201 ggaagcgatc ggtcacgatg ccgatgggct cgagttcgag ccccgaagcc gtgtggcgca
     1261 cgcttccact cacgaagcgt ggcggtcccg ttttggcgct cagggcaaga gccagttcgt
     1321 cgagcgcgcc gggcgcagcg acgtcgtagc tgcgaacgag gcgaacgcta ggcccggcgt
     1381 ggggcacgat ccgggcgctc aagcgctggg cccccggcgc gtagctcagg ccctccaccg
     1441 cggcgatctc gagcacgtag acgccgtcgg ccaagacgcg cgcgcggagt agcgtcggcg
     1501 gccgctcggc gagctcctgc gcgagctggt tggtgttgcg cacgcgcagc gcttcgggca
     1561 agccgctcca gtctccattg gacggcatga cgctcgttcg agcgactcgg ccttgcccca
     1621 aaacgagcag tcgattcgcg gcgcgccgag cgctggcagt cacgacttgc cccgtagcga
     1681 tcgcggcgag cgtggtacgc ggtgcaaacg gcttgcgccc gagttcactc ggcaagggcg
     1741 cgcgctgccc agctttgatc tcgtattcct tgcgcatgac cagcagcgtg gccgtatccg
     1801 gatcggccaa aacgacctca gccaagcgct ggctggcgtc tcctcgcaca cgcgcgccca
     1861 atgaagaaag gcgcagatgc gcgaggggag tttcgctcgc cacttcggtg cccaagatcc
     1921 gcgccgcggg cagcacgttg ggtcgccgca cggcgcgtgc gcgcgcgtgt agctcggaga
     1981 ttagcgccgc ggcgctcgcg gggctgtagc gagcgctgcg cgtgtggtac gccgacagct
     2041 cttgctccag atcctccaga atcagcgccg gccagatcag gcgcactgcg gtcaaggttt
     2101 ggcgaacgcg ctcgaattgg cctgccatcg agttgcgcga gtggatgata ccctcgagca
     2161 atagttcctg ggcgagcgcc agcgcaggtg agagttcgtc cgcgatggtc gagttctcgg
     2221 cgagcgcgtc gcgtacttcg agctcgaggc gctttgcgtg agagtcccta acgtcggcag
     2281 ctcggaaggc ccataccgcg agggccaccg cttccgccgc gcgctcaccg ctgtccgaat
     2341 gggcatagtt caaatcacgc ggcaccaaga agcgcacgct gcaggtcggg agcagggccg
     2401 tgggtgcgtg gtcgggtccc atcgcgcggc gcacctcggc gacgtaacct ttccggaaca
     2461 acgcgcgagc tctctcccag gcgcgcgccc cgagaaagtg ctcgagctct tcgtcgctga
     2521 agtctcccgg ggaccaagac gtcgtttcgg cgtccgtcga tttggcctca atccgctcgg
     2581 gagcacgggc ctgagtgtcg ggtgagttcg cgtcgggaga cttgccgtcc gccgaagcgg
     2641 ccgcgacgcg agcgtaggcc agcacgacga tgagcttgtg tcggcaaatg cccgcggcgc
     2701 cacagttgca aggcgcatca ctgaggcccg tccgctgagg aagccgcgtc tcgacgtctc
     2761 cgaagcgacc gattaccgtg ccgtccggga gctcctcgag cgtaggtgcc agacccgcgt
     2821 cgagctcctt ttgcgcgcgt ttgaccaacc cgcgattggc gagcgcggct agtgcgtctg
     2881 gcgtgagtac acgcaagtcc tcacggctca tcgctgcacc tgttccgcaa tccagctcgc
     2941 gagcgcgccc ggagtcatgg cgccgacgct cgcgcccacg ttgacgagct cttgcgcgag
     3001 cgcccggtca tagacggggt ttgcgtctgg gtcgagcgcg gccagcccta gcactttgct
     3061 gccttgctcg cacaacgccg aggtggcgcg caccagccgg tgtgcgtctc ctccctcgta
     3121 aaagtcgcta atcaacacga cgatggctcg gcgcggagct tcaatcaatg aagccgcgta
     3181 ctccaccgct ttggcgatgt cggtgccgcc tccgagttgc accttcatca gcaagtcgac
     3241 cggatccgcc acgtcggccg tcaaatcgac gacttgggtg tcgaacgcca cgaggtgggt
     3301 gcgcatcccc ggcaggcccc acaagcaagc cgccgtgacg gccgagtgga tcaccgagcc
     3361 caccatgctg ccgctttgat ccaccaccag gatgatctgc catcgttcgg tgaagcggcg
     3421 ggtgcgcgag aagaagcgtg gcttttcgag cgcgatgcgc tggagctcgg gctgatagtg
     3481 cttcaggttg gtttggatcg tgcgccggag gtcgaagttc ttggcggact tgtggagcga
     3541 ggggcgccgc cggtcgcgta cgccgctaaa cgacgcgcgc acctctttcg aaaggcgccg
     3601 catcagctcc tccaccactt tcgcgacgag cgcgcgagcc ttcgccagca cctctgggtt
     3661 catcaggtgc ttggtgtgga gcacggcgcg cagcagggac tcgctcggct cgatgcgctc
     3721 gagtacctcc aggttggtga cgacttcgtc gattccaaag cgctcgaccg catcgcgctc
     3781 cagccgctcg atggtttctt tggggaagag ccggtgcacc tcgttcaccc aatccggcgt
     3841 cgtgagctga gactcgccgc tgccgccggt gcgctgaact ccgcgctcgg cgagctcggg
     3901 atcacgcccg tacagccatt ccagcgcggc atccatggct tggccgcgcg gcgacaggtt
     3961 tggcatggct tgcccggcgg gggcgcccag cacgagccgc caccgctcga ggagctcgct
     4021 ctctttggga tcagccatgg acctccgctg gtgtcgtctg aagcgtcgac tcggttgacg
     4081 gttctaaccc aaagcgacgc tccagttcgt ccacacgagc ttcgagtgaa cgcgcggcgg
     4141 ccagcgtgtc ggcccccgcg gagagcttga ggaagtctcg ggcgtgcgca gttccctgtc
     4201 cgtgcaaggc cagcacgcgc agcgcgatgc gttcgcgttc gcggggcgga aaataggcgt
     4261 gcgccatgcg gagggctggc agcgcaacca aaaactcttc gagcgccatg tccgccaaga
     4321 gctcatcgag cgtggcaagc agagcacttt cgcccgcagt cacgatctcc tcccgggcga
     4381 gtgcgaacag ccccgccaag aagtccccca acgtcgcggg cagcgcggcc cgccgcagcg
     4441 cggaggaggc caacgcctct gccgtggtga gatcgtcgaa ggcatgcagc gaccataggt
     4501 acccgagcgc ggccccgcgc agcgcgggtg gagtcgccgc gtccgcgctc ttgcgatgca
     4561 tcatcgcggc cgcggagctc gcctcgaggt tcgctcgatc tcttaggaag cgcacgctgt
     4621 cacgtagtgc catgacgccg tggatcaacg tcgtgtcggc cggcgccgta ggccccgtga
     4681 gcccttcgaa cagccacaag ccgcgtgcga aggcggcttc gagcaccgca ccgagcgcgg
     4741 ggctgctttg cgagccaagc agcgtgtcgt gccggtaaag cgcgagcagg cgcgcttggg
     4801 ctttgcctag cttgccgaaa tcgggctcgc gactcaccag tggccccaac gtggtcagca
     4861 gctgcgagga tagccgttcg atgccaacga aggtggcttc ggcgagcagc gccgagactt
     4921 cgagcacgtc gggagcgccg cccaatcgct cctcgagtcg ggccaccgcg gccgtgtcca
     4981 gagtggctcc gtaagccgac gcttcgatca acgcgctctc ggcctcgaac gccccgcgca
     5041 gcgaccactc ttcttcgagc accggatccg ttccgtaggt tggcccgcta acccgttcca
     5101 aacctggaat cgcgagcaca cgcaggcgat gcagcgcacg gctcttgatt aagtctacgg
     5161 gctccgtgag cttcaaccgc accttacggg gtggcccctc gggccacaac ccgagctcag
     5221 tgagctccgc gcgcgccgcg accaacagcg gtggacgagg tgtttcggga gctaacctgc
     5281 cgctgcgctc gccactgagc gctgccacca cttcgactag gatcgggtca gtgcccggcg
     5341 gcagcttgcc tcgttgattc cacgggaaaa ccgcggtttg ggcgctcttg agcacggcgc
     5401 tcgcgaagcc gtcgagcaag tcacagcgcg acagcgcggc gtgcccgcgc aggcgagcca
     5461 agcccagggc catcgcggag gcgccgatca aatcggcggt gctgatggct tgtttctttt
     5521 tgcgcaggcg ccgcgtggcc tgctccagca tagcttgcgc ggcgtgttcc gggccgcact
     5581 cccacaccgc ttggtagtag gccggggagg gcatgccgga ctcgtaacct acgaagctat
     5641 ccaagcgttt gaacgagtac ggcaccaagt aactgccgtg cttggcgtct tcttccgccg
     5701 cagggaactc cggccaaacg ctgccgtccg cctccgagtg cagtctctct agctccggct
     5761 tgtgaaagcc gccgcacacc gccacgacat ctccgccctg gcgaatcgac cacgcgatac
     5821 agcgcgccat gtaggcttca cgtaacgcat cacgagtcat cgtctgggac acttcgcgca
     5881 tgcccgcgaa gtacgcttgc aagcgtgaac tcaacgcctc gagctccagc ggctgctcga
     5941 agagatggtc ccagagcgca tccatcccct cggcgccgaa ctggtcgcac aagctggcga
     6001 gcgcttgctc gtaacggcgc tcgtcgtccg cgtagcggtt gtgcaccccg tcgaacgcct
     6061 cggaccaggc cggcagatcc atgaagcgca cctcggcgcc gatctcacgt gccgtcgaga
     6121 gcgcgaccca ctctggcgag tactcgcaga acggcgacca agaagcgtga taacgccctt
     6181 cgctctgata gaacgaaaag atcgcgagcg gcagctgatg acggagctga agctcgctca
     6241 tgcgcgcgtt catgtccgcg ggcccctcga tcaacacgaa gcgcgggcgc agctggcgca
     6301 agcggtattc gaccaggcgt gcgcacgccg ggctgtggtg gcggacacca atcacggtca
     6361 gtgtcaccgt tgccctcagt cccccggcaa gagcttacgg gcttcgtaaa aggctcgcca
     6421 atgctcgccc tttttgctct tggcgcgttg ttcgaggtaa cggcggatgc gcttcaaatc
     6481 gtccgcgttg tctttcgcgg ccgtgcccgc caaacactcc actacgtccg ccggcgtgct
     6541 cgcgcttccg tgaaggtaga agccgcgcac gcccacggcg tgggccgtcg agacggcttc
     6601 ggcggtcgac atcaccgaac tcagccgctc catcgcctgc ccgtctttgg tctccccgct
     6661 gcgcagctcg cggaacgtag tgaccagcac ctcgagcacg tccggcgtga gcgcgtgcgg
     6721 cacgccggat tcccgcagca agcgcgccga ctcgctgcgg acgagctcga gctccgtcgc
     6781 gtagtccggg attgggaaca cggtctcgaa gttgaatcga cgcttgagcg ctgcgctcat
     6841 ttcattcacg ccgcggtcac gcgtattggc cgtcgcgatc acattgaacc cctcgcgcgc
     6901 aaacaccatc cccgcctccc ccgggagctc cggcaccgcc aagacgcggt ctgagagcat
     6961 actgagcagc gaatcttgca cttcgagcgg gcaacgcgtg atctcttcga acctcaccaa
     7021 cttgccctcg gacatgccgc gatagatcgg cgcaggcacg agcgcacgcg gggtgggccc
     7081 ctccgccacg agcaacgcgt aattccacga gtacttgatg tgatcctcgg tcaccgacgc
     7141 gccgccctga atcgtcagcg tggaggagcc gctcaccgcc gccgccaaga gttcggacaa
     7201 cagtgacttc gcggtgccgg gctcacccac caacatcaag ccacgattgg tggccagcgt
     7261 gaccagcgcc cggtccacca acgtgggctg tccaacgaac ttacgtttaa tccccgcgtc
     7321 cggatcgccc atgacaaacc gtcgtgccgc ctgcaatgac agctgccaac ccggggggtt
     7381 cggttggtcg tcgcgtgcgc gcagctgctc gagctccgcc gcgtagagct cttctgccgg
     7441 cgggcgctgc accttacgaa ccgggtcgct cgggctggga gtaggcttgg tcttgctcat
     7501 cgtccgtgtc tctgaatttg cgggtgcttt agaggggaag gggacagcgg ctagtcgcgc
     7561 agcgcctcca gatcgcggac cagctcgctg aacacactgg gtgacacttc cgagaacaac
     7621 gccttcgcgc gctcacttcc gctctccagg tgaagcttga gatccccaag ctcctgttcg
     7681 ggaggagtgc cctccatgta acccacgcag aagccccctt tcagctcgag ctcagcgcgg
     7741 aactcgccga gcggcttgta catgtcccac acccagccgg catcctgggg gacaccacgg
     7801 tgccaaccgc gcgcgtccag gcccaagatt ttgcccgttt tcacggtcac gccagtcagg
     7861 cgtttgagaa cgtcgccctt cagctcttct tccgtagggt ggaacacgga gcgaccgagt
     7921 tgttcgaacg gttgcagaat ctcgtagtca ccgaacactt gtccccactt cgcgcacgtc
     7981 gccgcgtcga gctccaaacg gtgtacgacc cccagtgtgg catgcggagg caactcgaaa
     8041 gtgtcgtctt cagagtcggc aaaggtgcga tcttccgcga cgcgaaacgt cgacgtcaac
     8101 ttgccgtctt ccgcgtaaac accccagagc acgcgccgaa ccaggtgaat catcagcgga
     8161 tgcgccacga agaagctccg aaatgcttct gcgctgaggc gccgcccggc gcacataatc
     8221 agctcaagcc gcaagatctg cgattgcgcg atggccttcg catccttctt caacgtcttg
     8281 tactgttcaa gggcggcgct cgctttctcg gcatcgtccg ccttaccggg tttaggcgga
     8341 tcgccgagcc gcttgcccgt cgcgtctcgc acgaagggct tgagctgttc gtcgaagccc
     8401 accacgaact gccgcgggcc gtagtccaac gtcagtgtcc cggtgttctc gtccaagccg
     8461 agatcgggca cgagtcgatc ggccaactcg aatgcggtga gcccgcgtgc ttccgccacc
     8521 acgtcgatct tctcgcgcgc tttctcttgc agccctttga acttgagctt ctgcgcaatg
     8581 ccgtgaaggt gcatcaacgc cacgtcggtc ccaatcgctg cgagcacgtc cagacccacc
     8641 accgcacgcg cgtgagcact ctcacctggc caagcgcgga tcatcggagt gagccgtcgt
     8701 gccgattcgt cattacccag gtgcccgacc gcccagaacg cccagacgtc cttggacggc
     8761 gcacccgcgc tgagccaggc actcatcaat tgccaggcga aatccgcggc ggcttcgggc
     8821 tcgagcgcgg ccaccacctc cgccaaaatc gagagcggcg cgtcgagcgg tgagagctgc
     8881 agcatcgtga tgaggtgctt gatggcgctc tccggcaagt ccccctttcc attcttcaaa
     8941 ctcaggggag gtaacgtctc gagctcgaag aatttgggcg tcgtcggcag cttggtcggg
     9001 tactcatcgg cgggcagcgc gagcagctgc ttcacggccg cgacggtcgc ctcgccgtac
     9061 ctctgggcaa ccctatttac ggcgtccgcg tggccgctcg cgatgacgcg cagcgccttt
     9121 tgcgcggcgg cccgctcgtg cttgtccgcg ccgaaggcga gcggcaacag gcccaatacc
     9181 gcgagctccg cgttcatctc cagccagtgc cgtgcgtcct tgcgcagcgt cttcttgtcc
     9241 agtgcgaaag ccaccgcgcg cacgaggcgc ggctcctgca ccttgctgaa cgtggagagc
     9301 acctcctgcg cgttgtccgc caagctcgcg acgcagcgtg gccagatgct ctcaccgaag
     9361 cgcgcaaaca aatagtcggc tggcatgtgt ggcagtgagc cgtcttcgag gccagccagc
     9421 aaaaactcgt cactcgcgag gtgcgcgatc ttgtccgcgt agtagttgtt cccgttcgcc
     9481 agcgccgcct tgaccagctt atccatctcc ggcgttcctg gcttggtctt gtccggaatc
     9541 ttggcctgct tccgagtgta gctatacgga tccatactct ggacgcgctg ggcgcggcgt
     9601 tgtttgctcc aatccggagg cacatacggc gaggccactt cgagctgcag agtcggaagt
     9661 gcttgcgagg tccgctgcgc gagccacggc ggcgagacca acacccgcgg aatgtctgcc
     9721 tcggcggccg gcgcgcgttt gcgcacggct tgaagctgcg cgatgcaagc gcgcgtccgg
     9781 ccatcgagcg ccgagcccaa gcgggcaggg agctcggggt gatcgcgcag cagccccttc
     9841 aacagaggct ccgcgtaagg cgacagcttc ccgccgctcg cgaccacctg accgagcacg
     9901 atcaagccca aatcaggccg tcgctcgaac agcgcgcgcg cgtgcggcgc ggcatccttc
     9961 ttatcgagta ccagggcgag agcgcgtccc gactctacgc tctccacgca agccagcgcg
    10021 cgcgccgcat cctcgcgcga atattgatta tcgagccact tgatgagcac gcccacacac
    10081 tcgagcccag cattcaccag cagcgacacg gggtgacgtc cgtactccgg cgtcggaaaa
    10141 acatccaaca ggcggcgatc ccgcagcaga tacaacaggt cacgcgcacc gtacacgtcc
    10201 tggtccttca gcgcgatcac ctccaaagca tcttcggcgg cccagcgcgt gtccgcgaac
    10261 atgagtgcga gcccaacgcg cgcaggcagt gccgcgctct tgcggagctg ttcagcgtgc
    10321 gcgtgagcgc gactacgagt gctttcgtcg gccgcggcga gcatccccgc cagcgattcc
    10381 acccgcttct cccccgagcc tgcgaacggc gtccagttcg tcgccggatc gcagcgtacc
    10441 acccactctg ggctgattcc cttcgaattg tgctggtgtt gccactcgtt ggcaaaactc
    10501 tccacgaaag cgtccagcgc atattcgagg ccgcgcgacg ccgcgaacag ccgcatgctg
    10561 taggtcgtgc gcatcagcgc tgcaaacgcc ccatccagtg ccgcgtccgg ctccttcggt
    10621 aatatccctt gcgcgtagct ttcgagcgcg tagtcggcaa cggctttgcc gtgcgcatct
    10681 gcatgactct tgctctcgat ccaagcctta tcagccttac gttgcttcac gatgaagtcc
    10741 cgcaacgaac tcaagagctc cggcaccttg acccctgtcg catccgggag ctcacgccaa
    10801 gcgtcgcgct cggcacgctg ctcgtctttc agcaccacca cgggttcggg attgaacgcc
    10861 tcgctcgacg tcgtcccctg ttcggggaca cgggtttctt caagtgcggg ggttgcttcg
    10921 gccgccgcgg ttggggagga cactgcggtc gcacccttac cctccgcacc cttaccctcc
    10981 gcacccttac cctccgcatc cttaccctcg tcgtggcctt gggctttgcc cttcaccttg
    11041 ttcgccgtgg gcgcaggcaa aggagcttcg ggtgctccca tggccgttac tccgctggtc
    11101 gcggcgccat tcacctcgat gtaccccttc tttttcttct ctgcgatgag cttgtccgct
    11161 tcgctcttgg ccttcgtctc gtcggcgaac tccttcgttt gcgcctgacc gttcgttcca
    11221 atctttcccc attggatcag cagcgagcca cccttctgct caacctgcca gaacttactc
    11281 gaactaccct ccgagaactc caaccgccgc atgccgatca cactatacgg ggttggcgct
    11341 caggtcattc ccaaatctcc acgagcctcc ccaacttacc cagtcctcca actcggccga
    11401 acgatcaacc gtcttggcac tgccccggaa tcctcgtgag gtctcgcagg ttcggtgaag
    11461 acctccctcc gcgccccctt cctcctacgg gtccgtgctg cagtcctcgc agagcacgtc
    11521 cgaaacctca tgtccggtga ccagttcgtt caaccacgtg gacaggcgca ctccagctac
    11581 ctgcagatcg taaaacttgt cgtcctcgac aatggtgtga gcactaccgg aactcgtaaa
    11641 actatgttgc acgatcccgg cttcttcgat gttgttttca ttctccatca tggaagccag
    11701 gagatcggat gtgtcttggc ccaacagcgt catgaagaag ccctggacgc ggtcactggc
    11761 gtagtcgaaa cgcgccatcg tcatattggg cgcgtgcagc cccgcctgca cccagaaccg
    11821 cgggatcccc caatcttgcg cttcgagccc ctcgttttcc tcccattgtg gcctagatgc
    11881 gaacgagccc cacttgttct cgaagatttc ggcgttgacg cgcggatcat cggggtaagc
    11941 ccccgaaccg tccgcaaaca cagtcactcg cgcgttcggc agccgatcgg agacgagacc
    12001 gccatagatg ggagaagcca ccgcgcctgc gcttgctccc acgaccacca cctgactcgc
    12061 gctcgcaaac tcgttactga gtgtctccag tgccgccgtg ccgttcacca tgccgcggtg
    12121 ttctaccgta agcgtctctg aatactcctg gctgatattg cccaaatgca catcgcccgt
    12181 gcagtaaggg acgtagacga acgagtagcc gagaaacgga ttgtccgcgc gcgcaaagtc
    12241 gaagatcccc ttttgctgcg ccgggtaatc ctcctctgaa atagtccagt cgtacaccgt
    12301 ttgggtcgcc gtgaacgcac aggtcaccgc gtcgaagcag gcgccgcccc cctgcaagta
    12361 caacacgacc tttcctgggt cccccaggta cgtccaaaac gaaaagtctt tcccatcggc
    12421 acacgccgta tccccaccgg gaagaacctg cttccacgta ttcgccgcga cctgctcgct
    12481 cgcatcctcc cccgagcacc ccagcgtgca aaccagactg agtccgcata tcgctgacgt
    12541 gagccgacct gttagacctg taagcgagaa gcgcggcgcg tgcatgcgcc gaagtctaac
    12601 gtcgaaagcc tgcaaaccgc catgctccac cagttctgca ctgcgaatcc cagtggactg
    12661 ccgcgtccgc gtcggaaccc gccagatatt cacgctgctg gtttcgctac gggtgccatc
    12721 acgtcgtgga cgcattcaac tcggcaagtc cgcccgacga gcggtgaatc ctggcggatc
    12781 ttggccgaga acgtcctggg tgcccgctgg aattggcctg ctagactggc cgaacatggc
    12841 cgactcagcc cggcgacgtg ctaattacga cgatcttcgc gacgttccgc ctcacttgat
    12901 cgcggagatc ctccagggcg agctcgtgac cagcccaaga ccgggccccc cgcatgccca
    12961 agcggcttcc agcttggctg gtgaactcta cgcgcccttt catcgtgggc gcggagggcc
    13021 tggcggttgg atcatcctga acgagccgga gctgcacctg cgcggtgaca tcctggtccc
    13081 ggacattgcg ggttggcgtc gcgagcgcat gccggccctt cccgtctcag ccgcattcga
    13141 gctcgcgcca gactggatat gcgaagttct atcggcatcc accgcgtcca ccgaccgagc
    13201 ggtgaaggta cctatttacg cgcgtgaaaa gattggccac atctggctcg tagatccggt
    13261 gttgcagatc ctcgaggtcc tccgtctcga cggccccgga taccgcattg ctggtgtttg
    13321 gcgcggcgat gtcaccgtcc agtgcgaacc tttcgagact cttccgatcc gcctcttgga
    13381 tttgtggtcc gtctagtgac gcgtcccaaa gtgacgatca ctcgatctcc gcggcgtagc
    13441 ctacttcatg ggacgccgca ctcgtcgcgt cagatacaca tcccttccga ggccccccgg
    13501 cggcaagtgt gacgtagctc gagcttgttg acgctccatc gcgcaccgat tgtcatgttt
    13561 cgtcccatga gagataaagg gaatgggatg agcttcacgc tcgtatagaa ccacggctct
    13621 ggacgtaccc ggagagcgcc gaacaccatg acgaactggg aagatagttg ccgccaacgg
    13681 cttgcatcac acctcgacga gaatggcgac ctcgagcccc cgtgggcgcg ttttccagat
    13741 tacgaacggt actgcatggg ttggcgaatg gggagcggcg aggactggct tggcctctgg
    13801 cacgtcttcg tcgaacaact accgcgggat gaagcgtcgc gactggccta tctccggcgg
    13861 cacccaccgg ctcccatcaa ttgggccgat cacgtggaaa acgtactgcg cttgtctccc
    13921 ctcgacgatg ctgacgagga cgaagagccg ccggacgaac ccacgcccgc tcgactcgaa
    13981 gaactcgagc agctcggact catcggttcc gacgtagcct tcaaaacctg gctcgagcgc
    14041 caaaggagtg agatctcgtg gccatggcga cacagcgaga cgcctgaaac tgcagcgcgc
    14101 tactccaccc gcccgctctg gttttggtcc cgacaaatcg ctcagctgag gccaactctt
    14161 gctctccccg agctcccgcc ggcctggcgc atctgcgagc aacctctact caccggatcc
    14221 ctcgcgtcga ttgatccgaa tcgaggtctc caatcgctcg cactcctatc gtgcgcgggc
    14281 aacgtgcgcc cgccatggca gtttgggctc agcctgagtg acttccgaga ttcattcgac
    14341 gacgacatgc cttatgtcga cgccttccgg ctttgggcca tctcctgcat ggacgacatc
    14401 gcccagctca ctcaactctg cgaacaactc gatgctcccc ccgcttggaa gtcgtggctt
    14461 ctggaacaat gcggcctaac gtcatgaatc caactgaccg cggccaataa tagatctcgt
    14521 gtgcgttgcc gaacgcacgg cttcatgcgc ccgtcgcgat aaccttgttg ggtggaccgc
    14581 tcgttgtaac gagcgcgaag tccgttcgcc gccgccgcaa gctcagccga acacgtcact
    14641 tccgctcgtc acaccgcgca agcggcacat tgccaatcgc caagacttga agccagacgt
    14701 cggggtcgcc cgggaaagtc gacgtcacac gagagtaggc agcgaacggc tttaattcga
    14761 agaagcggtt tagcgttaga tgcagccgcc aaaagaaagc gcaccaagaa gtcatgcgtt
    14821 gagagacgta gcgcacggag ttgtgggggc gcaaccttcg atcggtcatg cgtggtgaag
    14881 cagaggccac gcatggacgg ttcagcgacg cgccagtacg agctcgatgc gtcggttttg
    14941 ctggcggcct gcggcggtgg cgttgctcgc ggcggggcga tattgggcga agcccatggc
    15001 gacgagtcgt tcaccgggga gtttcccggt ctcttccaag aagcggacga cgttggtggc
    15061 gcgcgcggtc gagagttccc aattggtggg gaagcgctct tgagtcgtgg ggctgctgat
    15121 gcgggtgctg tcggtgtggc cacccacttg gagcgtgtag tccttgagtt gagcaagggt
    15181 tgggcccact tcgcctagaa ccttctgacc attctcgtgc acctcgggct tacccgattc
    15241 gaataggatc ttgtcggata gatcgacgac caagtcattg ccgcggcgcc gaatgcgcac
    15301 attaccggcc tgaatttcgg gctcgagttt cgcagtcagc tcgttctgga tgcgcttcaa
    15361 ctcagactcg atcttgtctc gctcggcttg cgtgtggacg agcttgccgg agagatcggc
    15421 atgcgactgc ttcagctcgg ccagctgcgc cgcggtttcg cggagctcct tcttcgagcg
    15481 ctgctcccgt tgcttctgct cgtcgacttg tgcttgcagc gcgccccgct ctcgacgcag
    15541 tggcagatac ccccgataaa cgaaggcacc cacgcccgcc gagaccgcca cgaaaagcac
    15601 ccagcccagc caccctgaac ccccacgccg tacgggttga ggtggcagat cgtactcgga
    15661 ccaaggaacg gccggtttgg gcatgatcgt atttgttgtt caggccgcgc tcgagagcaa
    15721 tccggacctg agcaacggcg tgcctcgcat cgagaggaag gcaatcgccc gcaggcgggg
    15781 ggcccattgg tccgaattta ctcgcgacac atcttgccca gccaggtaac ctgttgaaat
    15841 tagcggtatt ttagaaaatg agtagcggcg aaagaaaaag atcgagtgct tgtcgatctg
    15901 ggactttctc catcgtcgcc ctttcgagag caggcaatcg agccggcccg atccaagaac
    15961 gaagagaatc agccatgaag atcaccgtcg aaaccaacgt caaagccccc gtcgaccacg
    16021 tctggagagc gtacacgacc tcggcggaca tcaagcagtg gaacgccgct tcggatgact
    16081 ggcacaccac ggccgctacc gccgaccttc gcgaaggcgg agagttctcg ttccgcatgg
    16141 aagccaaaga cggcagcatg ggcttcgact tcgcgggcac atacacacgc gtcgtgaaga
    16201 acgagcggat tgaatatgcg tttggtgatc gtaccgctca ggtcgagttc caaccgagca
    16261 aagatggcgt tctcgtgcga gtcactttcg acgccgagtc cacccattcc gaggagcagc
    16321 aacgtttcgg gtggcaggcc atcttggata acttcaagcg ccacgtcgaa gcggccagca
    16381 agttgcaata acgagttggg ccagcgacta ctcgcgagcg aagcaccacc gctcatcgtc
    16441 cgactgctcg cagtcgggct cgccgtcgca gacgctcacc caaggaactg tggctccacc
    16501 ttcgcagacg aactgctctt ggatcgggcc acagtacctc tcgtcctccc cactggggca
    16561 ctccacctca ttgtcgcaat accaggcttt gctcccgtcc aagcaacgca cctgtgaggg
    16621 gaggcactga gcgtccaccg aagatgaccc ctcgacgtag cgagcgcagc ccagtcacgt
    16681 cagttccgcc catcacaccg cgcaagcggc acctcgtgaa ccgcgacgtt ggagccctcg
    16741 tcatggcctc gctgccgtaa cgtggtgcgc tgagcgcggt agggcgactg acagagcggc
    16801 agcgcaactg cgcctgggtc gcatcatgac gagtagtcgc gcacgtgatt gcgggagcgc
    16861 aaactcggat tggcaatgcg tgcgaagcag gtggtattgg ctgacgcggc gacggactgc
    16921 gctgttgcgc gtcgccaaat ggtcatcgta gatttacgat gggtcatgtc ttgtcgcgct
    16981 gcagcgtgca gcgccgcgct gagctcccga cacggagagc catctcaaaa gtccggcatt
    17041 tggggggctt aggcgaaggg tgaggcgggc cgccggtcgt gcagacttgc accgtggggt
    17101 catgattcgc gagccggcgg agccagcgtt agtgcgggtg tctggccgaa gtggatatca
    17161 tgctgaattt acagtcatta tcgacctgag tcagacaatc gataaaaaac gatgactcga
    17221 tcgacctctg caaaggccat cttctatccc ctcaaaccga tcttgatgcg cgcgtgctcc
    17281 accttgctgt acgtcaggct ccacgcgcga cgttccattg agcggtccgc ttcggcgacc
    17341 gctatgttca ggagtgtgcc ttccgacatc gcggcgccag gttcatcagt cgaagtcgta
    17401 aaagtcgggt ggcctatggt ccttcggaag atagatgagg gctaaaccgg ggcgcgggac
    17461 gtcgaagtaa agccagtcca tcattgcgaa aggatcttcg gtccaagcgt acccctgaaa
    17521 tgtgtcagca atgaagagag tcttgccgtc ctcgagggcg aaggcgacac cagttccggc
    17581 gtcaaaaatc gggtacacga aggaactccc tgccaacttg cagatcgcat ctgccgtagc
    17641 acaaggatcc attcgcatca gcttttcatc gaacccgagg ttcatgccat acgagaaagc
    17701 gatcccgttg ggctccactt ttcgccgcca cagggataat ccgctaaacg agcgaaggaa
    17761 ctcgtccgca tagtcattcg cgaccagaca cggcgcaagt tcttcagcgg gcggctcaac
    17821 ctgcctgcct tcccaccatc ctaccgcacg cagcgcgctg aacagttgcg ggtactccga
    17881 ttctgcccac gatacttcaa gcattcacga attcttccac gcttcgattc ggcgaggttg
    17941 tcagtccggc ttgatagaag tgagaggggc gctgacccct aggaagccac tgaaagacac
    18001 aatggccagg gtttgcccgg gactcttcag tctgcatggt aaaagtcgat gggtcgttga
    18061 tctctcggta gccactgcat agcacggccc ggtgtcggag tttcccatat cagccattcc
    18121 aacgcggtga acggatcatc ggtgagcacg aagccagtaa acacttcgct cacaaccaaa
    18181 gctctcccat cctctaagg
//
LOCUS       DASTKU010000461         2950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_756157, whole genome shotgun sequence.
ACCESSION   DASTKU010000461 DASTKU010000000
VERSION     DASTKU010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756157"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFQ61_15875"
     CDS             <1..205
                     /locus_tag="VFQ61_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9955987.1"
                     /translation="DSRGNAAKNEELSLRRAESVRAHLVTQGIPSDKTRAVGLGSARP
                     VADNNTAEGRANNRRVEIIVEPG"
     gene            complement(352..1812)
                     /locus_tag="VFQ61_15880"
     CDS             complement(352..1812)
                     /locus_tag="VFQ61_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9955988.1"
                     /translation="MDSSLEVQAGQLLAGKYRVERVLGQGGMGVVVAALHLQLDEQVA
                     IKFLLPAAQQNQEAIRRFEREARAAVKIRSEHVARVRDVGTLETGCPYMVMEYLHGRD
                     LGTLVSERGALPIEEAVDYVLQASEAIAEAHSLGIIHRDLKPSNLFHTLRADGSPCIK
                     VLDFGISKLTGMSASGSDLAMTRTTSVMGSPLYMSPEQMTSSRDVDARTDIWALGVIL
                     YELLSGHVPFSADTMPQLCAMILQQSAPPLRNVRPDAPEALQAVILRCLEKAPQHRFA
                     NIAEFVQALLPFCSRGGRTSIERISRTLSAAGLSTRNVEIPPSGAPGRTGSRTDAAWG
                     ATQGGARRRGPWIALGIALAVLGGGLVLARSLTTPSASKEAEAQGIAVNPAVGAAPSG
                     LASVEAPLPPPSAASSEAKVASIAPGAPAEKAPPVAANLGGKPVTARSPALGNPAQVA
                     NQPAKAVKKPPVAVPVNTPTVQPPRRDTSDLYNDRK"
     gene            complement(1960..>2950)
                     /locus_tag="VFQ61_15885"
     CDS             complement(1960..>2950)
                     /locus_tag="VFQ61_15885"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HET9955989.1"
                     /translation="PSLWEPRQLGRVLAALLETFPHEAQKLEITAECNPSSFDASVAQ
                     GFRAAGVNRISLGVQGLEQERLRFLGRLHDAGSALAALALATRHFENVSADLIFGVDR
                     QLPDVAASEVEIVVQSGVKHVSAYALTVEPGTQFGELQRRGRLPLLADELVAESFERV
                     ESTLESHGFEHYEISNYARAGRRARHNLGYWLGEPYLGLGAGAFGTLPRGGSTVRYRN
                     TPAIERYLAWADSDAPLVRELWSETSPWVASREDLSAETLLQERILLGLRLLEGLDVE
                     RAEAETGASFWTDVRKKGIDRWVNRGALRIDGSRLRLNKTHFLIADRVLVDLL"
BASE COUNT          524 a          946 c          968 g          512 t
ORIGIN      
        1 cgactcgcgc ggcaacgctg cgaagaacga ggagctcagc ctgcgacgtg cggaatcggt
       61 gcgagcccac ttggtgactc agggcattcc gtcggacaag acgcgcgcgg tgggtctggg
      121 atcggcacgt ccggtcgccg acaacaacac cgcggaaggg cgggcgaaca atcgccgcgt
      181 cgagatcatc gtcgaaccgg gctgacctct caccgccgat agctcgtggc ttcgcgacgg
      241 cggtgcgcag tcgggcgcac cgccgtgagc gagctatttc aagccgaaac cgccgccgag
      301 agatcggcgg gcgcgccgcg cggagcgacg acgcatcgct cgcggccgcg gctacttgcg
      361 gtcgttgtag agatcgctcg tgtcacgccg cggcggttgt accgtcggcg tgttcacggg
      421 taccgctacg ggcggcttct tgactgcctt ggcgggttga ttcgcgacct gtgccgggtt
      481 acccagcgcc gggctacgcg cggttaccgg cttgcctccc aggttggctg ctactggcgg
      541 cgccttttcg gcaggagcgc ctggggctat cgaggcaacc ttggcctcgc tgctggcagc
      601 ggatggcggg ggcagtggtg cttcgacgct cgcgaggccg ctcggcgctg cgcccacggc
      661 cgggttgact gcgatgccct gcgcctcggc ttctttactc gcgcttggcg tcgttagact
      721 gcgcgccaac accaggcctc ccccgagcac cgctagcgca attcccagcg caatccaagg
      781 gccgcgacgc cgagcgccgc cctgagtggc accccaagcg gcgtcggttc tgcttccggt
      841 gcggccaggg gctccgctcg ggggaatctc gacgttgcgt gtcgagaggc cagcggcaga
      901 gagcgtcctg gaaatacgtt cgatggaggt gcgtccgccc cgcgaacaga atggcaagag
      961 cgcctggacg aactccgcga tgttggcgaa gcggtgctgc ggcgccttct ccaaacaacg
     1021 caaaatcacg gcctgcagcg cttccggcgc atccggtcgc acgttgcgca gcggcggcgc
     1081 agactgctgc agaatcatgg cgcagagctg gggcatcgtg tctgccgaga agggaacgtg
     1141 cccggagagc agctcgtaga ggatcacgcc gagcgcccag atatccgtgc gggcgtcgac
     1201 gtctcgagag gacgtcatct gctcgggtga catgtacagc ggtgagccca tcaccgaagt
     1261 ggtgcgggtc atggccaaat ccgacccgga agcagacatg ccggtcagct tcgagatccc
     1321 gaaatccaag actttgatgc aggggctacc gtctgcgcgc agcgtatgaa acaggttgga
     1381 aggcttcaga tcacgatgga tgatgcccag tgagtgcgct tcggcgatgg cttccgaagc
     1441 ctggagcacg taatccaccg cttcttctat gggtaacgcg ccccgctcgc tgaccagcgt
     1501 gccgagatct cgcccgtgca ggtactccat gaccatgtac gggcagcccg tctctagcgt
     1561 tcccacgtca cgcacgcggg ccacgtgctc gctgcgaatc ttcacggcgg cccgcgcttc
     1621 gcgttcgaag cggcggattg cttcctgatt ctgttgcgcc gccgggagta agaacttgat
     1681 cgcgacctgt tcgtcgagct gcagatgcag cgctgcaacg actaccccca tgccgccttg
     1741 gccaagcacg cgctcgacgc gatattttcc ggccagaagc tgaccggctt gaacttcgag
     1801 agaggaatcc atggcggaag tggccgttga ctcgggtcgc gcggccggga gtcgggggga
     1861 aggttagcgc aaaagttcgt ccaaatccta ttgcagccgc ttactgtttc cgcggccaga
     1921 ggagctttat accccgcgag agcggaccac gggttcaatt cagagcaggt cgacgagtac
     1981 gcgatcagcg atcaggaagt gagtcttgtt tagcctgagg cgtgagccgt cgatccgcag
     2041 cgcgccccgg tttacccatc ggtcgatgcc tttcttcctg acatcggtcc agaagctcgc
     2101 gcccgtctcc gcctcggcgc gttcgacgtc gaggccctcg agcagtctca atccgagcag
     2161 gatgcgttct tgaagcaggg tctccgccga aaggtcctcg cgggaagcga cccaaggcga
     2221 tgtctccgac cacagctctc gcaccaaagg cgcatcggaa tctgcccagg ccaagtaacg
     2281 ttcgattgcg ggagtgttgc ggtaacgcac ggtcgaacca cctcgcggca gggtgccaaa
     2341 tgcccccgca cctaggccga ggtaaggttc ccccagccaa tagcccaggt tgtggcgggc
     2401 ccggcgcccc gctcgagcgt agttgctgat ctcgtagtgc tcgaagccgt gtgactcgag
     2461 cgtactttcg acccgctcga aagactccgc gaccagctcg tccgcgagga gaggcagacg
     2521 cccgcgtcgc tgcagctctc cgaactgagt accgggctcc accgtcaggg cgtaagcgct
     2581 gacatgcttc acgccgctct gaacgacgat ttcgacttca ctcgcggcaa cgtcgggtag
     2641 ctggcgatcg acgccgaaga tcaaatcggc ggagacgttt tcgaagtggc gagtggccag
     2701 cgcgagcgcg gccagcgccg atccggcgtc gtgtagacgg ccgagaaaac gcagtcgctc
     2761 ctgctccaga ccctgcacac cgaggctaat ccgattgacg ccggcagcac gaaagccctg
     2821 ggcaacgctc gcgtcgaagc tcgatggatt acactccgcg gtgatctcga gtttctgcgc
     2881 ttcgtgcgga aacgtctcga gcaaggccgc gagcacccga ccgagctgcc gcggttccca
     2941 caaggagggt
//
LOCUS       DASTKU010000462        19289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_756423, whole genome shotgun sequence.
ACCESSION   DASTKU010000462 DASTKU010000000
VERSION     DASTKU010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 19289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19289
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756423"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VFQ61_15890"
     CDS             complement(<1..506)
                     /locus_tag="VFQ61_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955990.1"
                     /translation="MALNRRTFLGAVPLCAVLAAACSGPLNSVTNTGLVELASTPSKG
                     NDKTILVFMPKTPQTDEVWKGLTDELSEDYQLIAIQVESGPGAAGALGQSIDRYKPVA
                     LVLMNNPTVNAYHEYQQKNPNRKFPPAVVVMASFLEGLTAKLVSTTGVSYEVPLITAM
                     TNFRRLIAL"
     gene            complement(497..1201)
                     /locus_tag="VFQ61_15895"
     CDS             complement(497..1201)
                     /locus_tag="VFQ61_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9955991.1"
                     /translation="MTSRDPLSRLVNAGAALAALALLSKTAHAGNAKLLVFLHLAVKQ
                     RALQGDIQAALPGVEVTAVGRIGDFERALKEGADAVMAVPPVLAAFNLSARLRGVRGG
                     ATEEKYCLVGVGAEPDPAKVATVGATDLLGRDKTTQFVKDIMGTAPHVERVSKVEDLL
                     PLLQMQRADAILMPSRLFSDIKSASKLALVQKELSKTVGLPSVAVVTPTGDPAVGSIA
                     KLPGAVMKTLGVDSWR"
     gene            complement(1278..3410)
                     /locus_tag="VFQ61_15900"
     CDS             complement(1278..3410)
                     /locus_tag="VFQ61_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9955992.1"
                     /translation="MRKTLTILTLLTAALAKPALAQAADSAPAVDDLDLVKLLNVSVS
                     TATKTAESVDEAPAVITVVTKEDIHRFGYRNMGEVLSHCVGFYSVDDHVQPNVAVRGM
                     TGGLGAQSSVIKIMIDGRSVADRTTSGNWLGAELIPLESVRQIEIIRGPASALYGADA
                     FLGVVNIITESPDESRPVRARLFFGVTGKNMSEQLDVVGNARIGRFDFLLGAIGERGS
                     RSGLELPVESPSPNLPEYVGERRTANNLDRTSLGLQARVGYRVPDVGHVIGSAYLSGF
                     SRGGDFAPWAQLTNGTDPEGRKLGTVISEGQLRLNLDTLYHAHKMVDLALQGTYSQRR
                     FLPGDRIEFGSDLWYARRRTSYRSGDAVAELRFFPTKRFNAIVGVEGVLDRESLRAPR
                     QIVRETEEAVAVGGASANRVTLSNVGAFVSTNVVVIERWLKLTGGLRFDHHSEYGNQL
                     TGRVGATSRLSDSLVLKLLYGSAFKAPSPYLLYASPMGPGDVLGNPALRPQKIHTVES
                     QLSFKPNPFIALTSGVSYNWLLDKAEFTPQGINQVAQNMASQKTFTWETRADVHHYDD
                     YSAYLGWEMVRSRRGLGEVGYASNLVGDANIVYPNYVARAGGAVSIPSPVDVPLSAGA
                     EGMFVGPRHAADASVLANGAQFDLDPYVTMNVFVTTRNLYLIPGHESVVALRAYNILG
                     ERGPDPGFSGFEYPLTQREVFLEFRHTY"
     gene            complement(3559..4692)
                     /locus_tag="VFQ61_15905"
     CDS             complement(3559..4692)
                     /locus_tag="VFQ61_15905"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9955993.1"
                     /translation="MRNSYGLTLAALAFAFSSAGCSSDSADSNVKAKKAIERTAVQES
                     IFKPKALEETVDALVSAIAKTEAQDLQLNVILKSVTGYWSPVVVGANRALGELEVSGA
                     VASPQQETGQEAIDEQNAMIASDRSLGSKGFGVAPFEKPVAEQIDASVTAGLPVVTID
                     SDLPESKRDMYIGTLNKEAGATAGNTLKGLLPAGPGTVIVLGHDDAGWQGGYERSMAA
                     KAVFESAGYSVTVRRTDWTTTGEASDLEALAGIFADADPPVVGMIGMFSNAYRCAMAA
                     EAAGRTADDIAIVAFDFDPKTVDYMRSGLIKATHAQRQYYMGYLTPYVLYGMNVLGKD
                     ATKAILGSQMVDEYRFNSGLDVVMSDQLDEYYSFLDGLGVGGS"
     gene            complement(4778..6127)
                     /locus_tag="VFQ61_15910"
     CDS             complement(4778..6127)
                     /locus_tag="VFQ61_15910"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HET9955994.1"
                     /translation="MTFPTSATPRRRSAWLQRGSRSLIGFALLAFGCGEDTKAPTGDA
                     IRIGVSLPFTGKEAALGRNLEQAILLAVQDVNDAGGIDGVPLELVSRDSNSGSERGLN
                     ELLDLLYNEQVAYLIGPEENHLANQIVADVKTLNVMNVLPGYAAPAIERSTTSGSWLR
                     LAPSSFAIGCAFATRAADDGAVSANTLASSEDYNASVSAAFTSEFRRNGGTAVPSVSV
                     SAGQSSYSSSLTKVFGPNPDKTFLIAYPATAATIMSEWAITGRRGSWYLSPLLRADAF
                     LSNVPYGALDGFLGMSPSLSLTSECEILEGDEVHGPVQCTQDNAARFTDHFAARWKGT
                     KPFPAAHLYYDAVVLLALGMQYAVATHGEIPKARVLQQTIRDMNVVTNDVAKWDDLES
                     ALSTVGQGKALRYAGAGAEYRFDAFGAADHRLFDTWTIGGREFVPTGTHFAACVQFD"
     gene            6453..8780
                     /locus_tag="VFQ61_15915"
     CDS             6453..8780
                     /locus_tag="VFQ61_15915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9955995.1"
                     /translation="MKSLRSKFIATMLLVTGLVGVATLVLVTILNTRASSQYLNAVRT
                     HIEDGIKSKGRVLTENHALAMRSLVLDNAYLDMQRLVERAVKEDTDLVYGLYVSSEGK
                     ALAYWQRGGSSSADKLVDKDVWRPLGLSEADLAVPALQIREANRLGETLLEVASPVQS
                     DDGERLGTIRYGLSTKRMQAALEAAQLDAKVRLERSLLMIGGIVGVCTLLGLLLSRVQ
                     AVRITRPVSDLTAAAERLAGGDRSVKVDIRSRDELEVLGASFNKMVQDLATSYNQLED
                     MNRTLEQKVQQRTAELGTKNRDMRLVLDNVDQGFITLAPNGSMAAERSRVVDDWFGAD
                     RGQSRTFWDFLAPHSEAFALSFHVAWDQLSDGFLPLEVAIDQLPAQLVLTKKTFSLRY
                     LPFMRDGEFEGVLVVVADVTEKLLKEREDAEQGELMQGFKRLMLDRSGFSNFLSEATE
                     MVEQIQAPEHPDMTLLKRTLHTLKGNSAMMGLVVVARLCHTLEDQLAETGEMSAETRD
                     ELVNRWKAIGEHISSFVGNNKQRVIEIPQSEYAALVSRLSAQTDREVLNELLSWQLEP
                     IATSFGRLSDQVHALARRLGKGEVNVVIDAPGVRIEPDVWSPFFSTLVHLVRNALDHG
                     IEPAEQRLAAGKAAAGTVLFRASPGPNTLTIEVSDDGTGIDWDRIARKGEELGLPVGT
                     HQELLAALMHDGVSTKSDISEVSGRGIGMSALRARVEEMGGTIDVRSARNVGTTWVIQ
                     LPWAGGSRAPSTRPRRSMPPRTSIAPASVSNGMAR"
     gene            complement(8898..10193)
                     /locus_tag="VFQ61_15920"
     CDS             complement(8898..10193)
                     /locus_tag="VFQ61_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HET9955996.1"
                     /translation="MTRLPNAVGPVSNTAACLIPSLLIASATLTCACSKPVEVKGESP
                     RVSPAGLSQAELELAVRGVNQFGLGILRGSGERNNAFCSPITIALGLAIPYAGAKGET
                     ATELGRALHGSMARGAFEKAMNRLSSELVNRNRAAVRSGDAEKKVEIHSARALWAQRG
                     AKFEKNYLDSLSGNYGVGVRLVDFAEQPKQTAREINDFVSDGTRGKIKELVSADTLSP
                     QAQLLVTSSLYLDANWKDEFSAGGTHPAKFTGLGGEPRNVNMMHRPGSYRYLARGELQ
                     AVELPYFGEQLSMLLILPEPGRFESVRAALAAELIEDVRRNLREEVLLLGLPKFELRL
                     GSRSLRSALESLGVRRAFEQGADFTGITQKPLNISQVVEQVYVAVDERGTQAASAVAL
                     EMAAASAPPEKVVEFNRPFFSFIVDTTGVLLFAGQVTEP"
     gene            complement(10244..10831)
                     /locus_tag="VFQ61_15925"
     CDS             complement(10244..10831)
                     /locus_tag="VFQ61_15925"
                     /inference="COORDINATES: protein motif:HMM:NF025611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4347 domain-containing protein"
                     /protein_id="HET9955997.1"
                     /translation="MAGYNFRRGRQMIVHDNRLNNPFTSPPTSLRPPAAENVWTVMGA
                     EPAEHILGWTAQVAKGNSGSGKLNALHFMAHGFPGGIQLGRDNLSWSNVQLFQKLRGC
                     ISGAIVFFSCQVGAEQANHSPSYQLTFGNAVAVNAGTTVVACQLNQWYSFGSSTIDFG
                     DFEGVVYVYNARGEGAQLQNYSSRSKVNLERLVFG"
     gene            complement(10908..11852)
                     /locus_tag="VFQ61_15930"
     CDS             complement(10908..11852)
                     /locus_tag="VFQ61_15930"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9955998.1"
                     /translation="MELDTLRLFIRAEERGNLSAAARELGWLPATASAALKRLEQELG
                     GRLFDRTTRSLRPTQQGKQYLERARQAVTTLNEARELFQFSKTRVEGLIRLSAPVDIG
                     EQLVVPALDRFLETHSRIELALQLSDQMRDLWRDDFDAAIRYGTPHEGSLVVRKLAET
                     RRVLVAAPSYVERHGRPSRIEHLNQHECLLLKTSARSASNWRLVRGKRVVDVKVKGRR
                     LTDSGAVTRRWALAGHGITLKSWLDICEDVAQGRLLHLLPDVASESYPVMLATASGVR
                     LVGRMRALGDWLADAFADRITRHPVPFLLHSSTWKRAR"
     gene            11968..12984
                     /locus_tag="VFQ61_15935"
     CDS             11968..12984
                     /locus_tag="VFQ61_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HET9955999.1"
                     /translation="MKAVALTRYLPISEPESLMDVELEKPQPGPRDLLVRVQAVGVNP
                     VDCKVRAPKPKIENAPRVLGWDAAGVVESVGAEVTLFRAGDAVYYAGDITRPGTNQQF
                     HCVDERIVGHKPRTLDFAEAAALPLTAITAYEALFERLALDIDGADAGSSLLIIGAAG
                     GVGSIAIQLAKLAGLRVVATASRESSRSWVKSLGADEVIDHTRELRDELTRIGVSEVD
                     AIAVFSDTDRYFPALPALIKAQGRITSIVETSRHVDLELLKSKSLTFVWEFMFTRSMH
                     RTPDMIEQHRLLDRVAELVDSGKLRSTLTERLSPIDAASLRRAHAALESGHTIGKIAV
                     EGWR"
     gene            complement(13050..13271)
                     /locus_tag="VFQ61_15940"
     CDS             complement(13050..13271)
                     /locus_tag="VFQ61_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956000.1"
                     /translation="MAMPTPPGHPSSPTPEPAEVPRPETPAEVPLPAPSREEPPPMRD
                     PEPEPAPAPLEDPPPETPPGEPPPGPAIS"
     gene            complement(13506..13592)
                     /locus_tag="VFQ61_15945"
     tRNA            complement(13506..13592)
                     /locus_tag="VFQ61_15945"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13556..13558),aa:Ser,seq:gga)
     gene            complement(13740..13826)
                     /locus_tag="VFQ61_15950"
     tRNA            complement(13740..13826)
                     /locus_tag="VFQ61_15950"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13790..13792),aa:Ser,seq:cga)
     gene            14011..15540
                     /locus_tag="VFQ61_15955"
     CDS             14011..15540
                     /locus_tag="VFQ61_15955"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET9956001.1"
                     /translation="MSLFGRFGLVLPLGLWVILVAALFRWVPGVPRKRLRRSVILYGA
                     YASLLSVSWVLTHFKASNGWSGALLAAAALVELFLIINLAALTLFDFLAGWVRVKLSD
                     ILHDLAVGVAYLVAIVWSMHSWGVNLTGIVATSAVATAVIGLSLQSTLGNVIGGLALQ
                     LDDSIKEGDWVELENKTQGRVQSVRWRHTVLETRDHDTLIVPNSMLLNQTIKVLGKRA
                     GEPLVHRMWVYFNVDHRFAPGAVIRVVDEALRAASIENVAEVPAPQTICYDLAREHRD
                     SYCYYAVRYFLTDLARDDPTSSEVRERIYAALRRAGMPLALPAATLFLSHEDQKSERR
                     LNKVKAEIHAALRGVDLFSKLSEEELDILAESAKHMPFVRGEIITRQGAKAHFLYVLV
                     KGEVEVRVSAEDEDRRVAVVVAPGFFGEMALMTGAPREATVVSLTDVECLRVDKDDFR
                     AVLARRPEIAQEISTLLAQRRVELSAVRDNLDADAKRRRMQTERGRILAAIRDFFALE
                     E"
     gene            complement(15600..17105)
                     /gene="gcvPB"
                     /locus_tag="VFQ61_15960"
     CDS             complement(15600..17105)
                     /gene="gcvPB"
                     /locus_tag="VFQ61_15960"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET9956002.1"
                     /translation="MSHHTALAEADRGLEFTEPPIFERGAPGRSAVSLPELDVPAVDS
                     RAVLGSLARGTPAGLPEVSEPEVVRHFVRLSQKNFSIDTQFYPLGSCTMKHNPKVNEW
                     AARLPGFAGLHPLLPDPLIQGALELMVRLEGLLAEIVGMDGVSLQPAAGAQGELTGLM
                     MIRAYHTDQGRAPRKVFIPDSAHGTNPASCALNGFEAVPFPAGSAGVVEPETLLAAVE
                     AAGSDVAALMITNPNTLGLYEQAMPKLSALIHERGGLVYGDGANLNAVLGRARPGDAG
                     VDVMQFNLHKTFTTPHGGGGPGSGPVAFKKHLEPFQPTPVVRRNGDRYVVDHTRPKSV
                     GRVRSFFGNFGMMVRAYTYIRELGPEGLRRVSDMAVLNANYVAAKLRDHLPLAFETPP
                     LHEAVFTDRELEAETSVKTLDLAKRLIDYGFHPPTVYFPLVVRGALMVEPTETESKQT
                     LDAFVEAVVAILREARTTPELVQKAPHLTQLGRLDEARAARKPRLRWSPVE"
     gene            complement(17157..17738)
                     /locus_tag="VFQ61_15965"
     CDS             complement(17157..17738)
                     /locus_tag="VFQ61_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956003.1"
                     /translation="MQGSRVLRIASRLLLIAPLLFSVSCGGGQNQVKHANVTAGTMPE
                     GGNWQGVYYSQLYGYLHLLSDGSSANGAWRTTAGDAYGELHGQIEGDLLRFEWVERRI
                     GAVGADANRKGKGYFRYTSPKEGEAHEIVGEWGLGEADAGYEWRAVKQKNMPPNPASV
                     KPDEVEGRVTGVDSWDDGGEEGSDKKDSDSDSK"
     gene            complement(17757..18671)
                     /gene="hemC"
                     /locus_tag="VFQ61_15970"
     CDS             complement(17757..18671)
                     /gene="hemC"
                     /locus_tag="VFQ61_15970"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET9956004.1"
                     /translation="MAKHKLVLATRKSALALAQSRAFAKTVQALHPKLEIEELTVTTT
                     GDRVQDRLLSEVGGKGLFVKEIEEALLDGRADLAVHSLKDVPAEMPSGLVIGCYPRRE
                     DPRDVLISRTGAKLVELPAGSVVGTSSLRRRMQLEKARPDLRIVGLRGNVDTRLRKCR
                     EGEMDAIVLARAGLLRLGLTAEVTEVLEPEIMLPAVGQGALGIEYRAADAEVAALLDP
                     LSHPDTKIAVLAERATLKTVEGSCHLPVAAFAMRDGNQMFLRGFLAEADGSRAREHAL
                     REAWPSSEVEAEGLGQRLGEVLARLDAR"
     gene            complement(18653..>19289)
                     /locus_tag="VFQ61_15975"
     CDS             complement(18653..>19289)
                     /locus_tag="VFQ61_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956005.1"
                     /translation="ARKSRRGRAQTFIDLAVPRDVDPAGGELDDVFIYNIDDFSRVVA
                     ETLSARSREAELGERIVDEETRGYERWAEAEQATPLIVRLREEVRRALRLELERSTRG
                     RLKHLGLTDREALETMLDAAANRILHAPTMRLREAAQDRKSGTLSFDDLSLALSELFG
                     LAEGRADPEMELVSQPRAAGPSGTPGALGDSIAGDEKSHVTGTLGKWPNTS"
BASE COUNT         3465 a         6210 c         6070 g         3544 t
ORIGIN      
        1 agcgcgatta gacgccgaaa attggtcatc gcggtgatca gcggaacttc gtagctgacg
       61 cccgtcgtgc tcaccaactt cgcggtcaag ccctcgagga aggaggccat caccacgacg
      121 gcggggggga acttccggtt cgggttcttc tgttggtatt cgtgataggc gttcaccgtc
      181 gggttgttca tcagcacgag cgcgacgggc ttgtaacgat cgatgctttg gccgagcgcg
      241 ccggccgcgc cgggcccgga ttcgacctga atggcgatca gctgatagtc ctcggacaac
      301 tcatcggtca gccccttcca gacctcatcg gtctgtggcg tcttcggcat gaacaccagg
      361 atggtcttgt cattgccttt ggacggggtg ctcgcgagct cgacgagccc ggtattcgtg
      421 accgaattga gcggcccgga gcaggcggcc gccagcaccg cgcagagcgg aacagcgccc
      481 aagaaggttc ttcgattcag cgccatgagt ccacccccag cgtcttcatg accgcgcccg
      541 gtagtttcgc gatgctgccg accgccggat cacccgtggg tgtgaccacc gcgaccgacg
      601 gcaacccgac tgtcttgctc agctcttttt gaacgagggc gagcttgctc gccgacttga
      661 tgtccgagaa gagcctggac ggcatcaaga tcgcgtccgc tcgctgcatt tgcaacagtg
      721 gtaacaagtc ttcgaccttc gatacgcgct cgacgtgcgg cgccgtcccc atgatgtctt
      781 tgacgaactg agttgttttg tcgcgcccca acagatcggt ggcgcccacg gtcgcgacct
      841 tggccgggtc tggctcggcg ccgaccccga ccaagcagta cttttcttcg gtcgctccac
      901 cgcgtacgcc ccggaggcgt gcgctgagat tgaaggctgc gagcacggga ggaacggcca
      961 tcaccgcgtc tgcgccttcc ttcagggcgc gctcgaagtc gccgatgcgg ccgacggcgg
     1021 tcacctcgac ccccggcaga gcggcctgaa tatcgccttg gagggcgcgc tgcttgaccg
     1081 cgaggtggag gaaaacgagg agcttcgcgt tgccggcgtg cgcggttttg ctgagcaacg
     1141 cgagcgcggc gagcgccgcc cccgcgttga cgagacgtga caacggatca cgagaagtca
     1201 tccgggcctc gcttgggatc tgaattgaga ctttccactg ctctccatgg aatcagctcg
     1261 cgtgctagta cgcgccctca gtaggtgtgc ctgaactcca ggaacacttc gcgttgtgtc
     1321 agcggatatt cgaagccgga gaacccgggg tcaggcccgc gctctccgag gatgttgtac
     1381 gcgcgcagcg cgaccaccga ctcgtgcccg ggtatcaaat acaggttacg agtcgtgacg
     1441 aacacgttca tggtcacgta cgggtccagg tcgaattggg cgccgttggc gagcacgctc
     1501 gcatcggcgg cgtgtcgcgg cccgacgaac atgccctccg cgcccgccga aagcgggaca
     1561 tcgaccgggc tcgggatgga gacagctccg cctgcccgcg cgacgtagtt ggggtatacg
     1621 atattggcgt cgccgaccag gttcgaggca tatccgacct cgccgagacc gcggcgcgag
     1681 cgcaccattt cccagcccag gtacgccgag taatcatcgt agtgatgaac gtcggcgcgc
     1741 gtctcccagg tgaaggtctt ctggctcgcc atgttctgag ccacctggtt gataccctgc
     1801 ggagtaaact ccgccttgtc caacagccag ttgtaggaga cgccgctggt cagcgcgatg
     1861 aaggggttcg gcttgaagga cagctgcgac tcgacggtat ggatcttttg cggtcgcagc
     1921 gccgggttgc cgagcacgtc gccgggaccc atcggggacg catacaacag gtatggggat
     1981 ggcgccttga acgcgcttcc atacagcagc ttgagcacca gcgaatccga cagacgagag
     2041 gtcgcgccga cgcgccccgt cagctgattg ccgtactcgc tgtgatgatc gaagcgcaga
     2101 ccgcccgtca gcttgagcca gcgctcgatc acgaccacgt tcgtgctgac gaaggcgccc
     2161 acgttggaga gggtgacgcg gttcgcgctc gccccaccga ccgccaccgc ttcctcggtt
     2221 tcgcgcacga tttggcgggg agcacgcaag gattcgcgat cgagtacgcc ttcgacgccg
     2281 acgatggcgt tgaagcgctt ggtcgggaaa aagcggagct ccgcgacggc gtcgccgctg
     2341 cggtacgaag ttcgtcgtcg cgcataccag aggtcgctac cgaactcgat gcgatcgccg
     2401 ggtagaaagc ggcgctggga gtaggtgccc tgtaaggcca aatctaccat cttgtgcgcg
     2461 tggtagaggg tatccaggtt gaggcggagc tggccctcgg agatcaccgt gcccagtttg
     2521 cggccctcgg gatcggtgcc gttcgtgagc tgcgcccaag gcgcgaaatc accgccccgg
     2581 ctgaagccgg atagatacgc actcccaatc acgtgcccga cgtcgggcac gcgataaccg
     2641 acgcgagctt ggaggcccag agaggtgcga tccaagttgt tggcggtgcg gcgctcaccc
     2701 acgtattcgg gcaggttcgg gctgggcgac tctacaggta gctcgagccc ggagcggctg
     2761 ccgcgctcgc cgatcgcgcc gagcagaaag tcgaagcgcc cgattcgagc gttgccgacg
     2821 acgtcgagct gctcgctcat gttcttgccg gtcacaccga agaacagtcg agcccggacc
     2881 ggacgcgact cgtccgggct ttcggtgatg atgttgacga cgcccaagaa ggcgtctgcg
     2941 ccgtatagcg cggaggcggg accgcggatg atctcgatct ggcgcacgga ctcgagcgga
     3001 atgagctccg cgcccagcca gttgccggac gtggtgcggt cggccaccga gcggccatcg
     3061 atcatgatct tgatgacgct gctctgcgca cccaggccac cggtcatacc acgcacggcc
     3121 acgttcggtt gaacgtggtc gtcgacggaa tagaagccga cgcagtggct gagcacctca
     3181 cccatgttgc gatagccaaa gcgatgaata tcctctttgg tcacgacggt gatcaccgcc
     3241 ggcgcctcat ccacgctctc ggccgtcttg gttgccgtcg agacgctcac gttgagcagc
     3301 ttgacgagat cgaggtcgtc tacggccgga gcgctgtccg cagcttgcgc gagcgccggc
     3361 ttcgccagag ccgcagtgag gagcgtcagt atcgttaggg tctttctcat attgcaccag
     3421 gcgcgagcag tccgcatttt gcgatcgcag accgttcgcc ccgcacgagg cgaagcgcac
     3481 aggcacttca cctcacgggc gcggggcgaa cacgaagcgc ttcgcctcgc tgacgcgagg
     3541 cgaacacgga tcgacagctc agctccctcc gacgcccagg ccgtccaaga agctatagta
     3601 ctcgtcgagt tggtccgaca tcacgacgtc gaggccagag ttgaagcggt actcgtccac
     3661 catctgtgag ccgaggatcg ctttggtggc atccttgccg agcacgttca tgccgtacaa
     3721 cacgtagggc gtgagatagc ccatgtagta ttggcgctga gcatgcgtgg ctttgatcag
     3781 gccgcttcgc atgtaatcca ccgtcttggg atcgaagtcg aaggcgacga tggcaatgtc
     3841 atccgcggtg cggcccgcag cttcagcggc catcgcgcag cgatacgcgt tcgagaacat
     3901 accgatcatg ccgaccacgg gcgggtctgc gtccgcgaag atgcccgcaa gcgcctcgag
     3961 atccgaagct tcgccggtcg tggtccaatc cgtgcgtcgc accgtcacgc tgtagccggc
     4021 gctctcgaac accgccttcg ccgccatcga tcgctcatag ccgccttgcc aacccgcgtc
     4081 gtcgtggccc agaacgatga ccgtgccggg cccggccggg agcagacctt tcaacgtgtt
     4141 accagcggtg gctcccgcct ccttgttcag cgtgccgatg tacatgtcgc gcttggactc
     4201 ggggaggtcg ctgtcgatgg tcacgacggg aaggcccgcc gtcaccgacg cgtcgatctg
     4261 ctcggcgacc ggcttctcga agggcgctac gccaaagccc ttgctgccga gcgagcgatc
     4321 ggacgcgatc atggcgttct gctcgtcgat ggcctcttgc cccgtttcct gttgcggaga
     4381 agccacggcg ccgctcactt cgagctcgcc gagcgcgcgg ttcgcgccca cgaccaccgg
     4441 tgaccaatag ccagtgacgc tcttcaggat gacgttcagc tgcaggtctt gcgcctcggt
     4501 cttcgcgatc gcggagacga gcgcgtcgac ggtctcttcc agcgctttcg gcttgaagat
     4561 gctctcctgc acggccgtgc gctcgatggc cttcttggcc ttgacgttcg agtctgcact
     4621 gtcgctgcta catcccgcgc tggaaaatgc gaaggccaac gcggcgaggg tcaatccata
     4681 gctatttctc atctctaact gttctttcgt ggcggtgggg ggttgttggg gtgaacggtt
     4741 tcgtgctcgg tggggaggcc cgcggcgtga agctcgatca gtcgaactgc acgcatgcgg
     4801 cgaagtgcgt gcccgtcggc acgaactcgc gtccaccaat cgtccaagta tcgaagagac
     4861 ggtgatcagc ggcgccaaag gcgtcgaatc gatattccgc gcccgcgccc gcgtagcgta
     4921 gggccttgcc ctgaccaacg gtcgagagtg cgctctctag gtcgtcccat ttggcgacgt
     4981 cgttcgtaac aacgttcatg tcgcgaatgg tttgctgcag cactcgggcc ttcgggatct
     5041 cgccgtgcgt cgcgaccgca tactgcatac cgagcgcgag caggaccacc gcgtcgtagt
     5101 acaggtgggc cgccggaaac ggcttggtgc ccttccagcg cgcggcgaag tgatcggtaa
     5161 accgagcggc gttgtcttgg gtgcactgca cgggaccgtg gacctcgtcg ccttccagga
     5221 tttcgcactc gctcgtcagg ctcaaagatg gcgacatgcc gaggaagccg tcgagcgcgc
     5281 cgtacggaac gttcgagagg aatgcgtctg cgcgaagcag cgggctcaag taccaagagc
     5341 cgcggcggcc cgtgatcgcc cactccgaca tgatggtcgc ggcggtcgcc ggatacgcaa
     5401 tcaagaaggt tttgtcgggg ttggggccga acaccttggt tagcgagctg gagtaggagc
     5461 tctggcccgc gctcacgctg acggacggta cggcggtgcc tccgttgcgg cgaaattcgc
     5521 tggtaaacgc ggcggagacg ctggcgttgt agtcctccga ggaggccaaa gtgttggcgg
     5581 acacggcgcc gtcgtccgcg gcccgggtcg cgaacgcaca accaatcgcg aacgaagaag
     5641 gcgcgagccg gagccatgaa ccggacgtgg tcgagcgttc gatggccggg gccgcgtaac
     5701 ccggcaacac gttcatgacg ttcagggtct tcacgtcggc gacgatctgg ttcgcgagat
     5761 ggttctcttc aggcccgatc aggtacgcga cctgctcgtt gtagagcaga tccaagagtt
     5821 cattcagacc gcgttcggag ccagagttgc tgtcgcggct cacgagctcc agcggaactc
     5881 cgtcgatgcc gcccgcgtcg ttcacgtctt gaaccgcgag cagaatcgct tgctccaagt
     5941 tccgtccgag cgccgcctct ttgccggtaa acggcagcga aaccccgatt cgaatcgcgt
     6001 ctcccgtcgg cgctttggta tcttcaccac atccgaacgc gagaagggcg aagccaatca
     6061 gggagcgaga gccgcgctgc agccacgcgc tccgccggcg tggggtggcg ctggtcggaa
     6121 atgtcacgtt cagcgtcaac gactaaacgt cagtactttt taagttgatt gttggagtcc
     6181 tgacattctc gaccgtctca tgcagagcgc accggcgcgc gctatgtggc cagcaaacga
     6241 agagcgcgaa cggcgctctg agattcgtca tgcgccgcgc ccgagtggga agtgggcagc
     6301 gcgcgcagag caacacacgc caatccgaag cgggccgtga tcaacgttaa cccgaactcg
     6361 cccgcagccg ttcggccgtg caggcacgat cgctgggcgc aagaaactcc cctcgacgcg
     6421 aaggccaacg ctctcgaaat gaaggcaaat cagtgaaaag tctgcgcagt aagttcatcg
     6481 cgacgatgct cctggtgacg gggctcgtcg gagttgcaac gctggtgcta gtcaccatcc
     6541 tgaacactcg tgcgtcgtcg cagtacctga atgcggtgcg cacacacatc gaggatggca
     6601 tcaagagcaa aggccgcgta ttgaccgaga atcacgcgct cgccatgcgc tcgctcgtgc
     6661 tcgacaatgc ctatttggac atgcaacgtc tggtcgagcg tgcggtgaaa gaagacaccg
     6721 acctcgtgta cggcttgtat gtcagctcag aaggcaaagc cctcgcctat tggcagcgtg
     6781 ggggcagctc gagcgcggac aagctcgtcg acaaagacgt gtggcgtccg ctcggcttgt
     6841 cggaagccga cctggcggtt cccgcgctgc agattcggga ggccaaccgc ttgggtgaaa
     6901 ccctgctcga ggtcgcctcg ccggttcaga gcgacgacgg agagcgcttg ggcaccattc
     6961 gctacgggct ctccaccaag cgtatgcaag ccgcgctgga agccgcccag ctcgacgcca
     7021 aggttcgcct cgagcgctcc ttgctcatga tcggcggcat cgtcggcgtg tgcacgctgc
     7081 tcggtttgtt gctgagccgg gtgcaggccg tgcgcatcac gcgcccggtc tcggatctca
     7141 ccgcggcggc ggagcgcctc gccggcggcg atcgctcggt caaggtagac atccgctcgc
     7201 gtgacgagct ggaggtgctc ggcgcttcct tcaacaagat ggtccaggat ctagcgacct
     7261 cctacaacca gctcgaggac atgaaccgca ccctcgagca aaaggtccag cagcgcaccg
     7321 ccgagctcgg gaccaagaac cgggatatgc gcctggtgct cgacaacgtg gatcaaggtt
     7381 tcatcacgct ggcgcccaat ggctcgatgg ccgccgagcg ctcccgcgtc gtcgacgatt
     7441 ggttcggagc ggaccggggt caatcgcgca ccttctggga cttcctcgcc ccgcactcgg
     7501 aggccttcgc tttgagcttc cacgtggctt gggaccagct ctccgatggc ttcttgccgc
     7561 tcgaggtggc gatcgatcag ttgccggccc agctcgtgct caccaagaag actttcagtc
     7621 tgcgctacct gccgttcatg cgagatggtg agttcgaggg cgtgctggtg gtggtcgccg
     7681 acgtgaccga gaagctgctc aaagagcgcg aggacgccga gcagggcgag ctcatgcaag
     7741 gcttcaagcg cctcatgctg gatcgcagcg gcttctccaa cttcctcagc gaagcgacgg
     7801 agatggtcga acaaatccaa gcgcccgagc atccggacat gacgctgctc aagcgcaccc
     7861 tccacacctt gaagggcaat tcggccatga tggggctcgt ggtcgtcgcg cggctctgcc
     7921 acacgttgga agaccaactc gcggagacgg gggagatgtc ggcggaaacg cgcgacgagc
     7981 tcgtgaaccg ttggaaagcc atcggcgagc acatttcgag ctttgtcggc aacaacaagc
     8041 aacgcgtgat cgagatcccg cagtccgagt acgccgcact ggtatcgcgg ctctccgcgc
     8101 agaccgaccg cgaggtactg aacgagctgc tctcttggca actcgaaccg attgccacct
     8161 ccttcgggcg cttgtccgac caggttcacg cgctggcgcg aaggctcggt aagggcgagg
     8221 tcaacgtggt aatcgacgcg ccgggggtac gcatcgaacc cgacgtttgg tcacccttct
     8281 tctcgaccct cgtccacctc gtccgcaacg ccctggacca tggcatcgaa cccgcggaac
     8341 aacggctcgc cgcgggcaag gccgccgccg ggacggtact gttccgcgcc tcacctggcc
     8401 ccaacactct cactatcgaa gtcagcgatg acggcacggg catcgattgg gatcgcatcg
     8461 cgcgcaaagg cgaggagctc gggttgcccg tgggcaccca ccaagaattg ttggctgcgc
     8521 tcatgcacga cggagtgagc accaagtccg acatctcgga ggtgtcgggc cgtggcatcg
     8581 gtatgagcgc gctacgcgcc cgggtcgaag aaatgggcgg caccatcgac gtccgttccg
     8641 cccggaacgt tggcaccacc tgggtcattc aactgccctg ggcgggcgga agtcgtgccc
     8701 ccagcactcg cccgcgtcgc agcatgccgc cccggacctc catcgcgccc gcgtccgtat
     8761 ccaacggaat ggctcgctag cagtctacta agagcgcgcg aggctcgagc cggaatcaac
     8821 tgggcagcag gttgctgccc agttgccatt ttaggcagca ttctgccgcc cgcagccgtt
     8881 ctagctccga gcagcgctca gggttcagtg acttggccgg cgaagagcag aactccggtc
     8941 gtgtcgacga tgaaagagaa gaatggccga ttgaactcga cgaccttctc ggggggcgcg
     9001 ctcgcggccg ccatctccaa tgccaccgcg gaggccgcct gcgtcccgcg ctcgtcgacc
     9061 gcgacgtaga cttgctccac gacctgcgaa atgttgagcg gcttctgcgt gatgccggtg
     9121 aaatccgcac cttgctcgaa ggcgcgtcgc acgccgagcg attccagagc actccgcagg
     9181 ctccgggacc ccaaacgcag ctcgaatttg ggcaagccca ataggagcac ctcttcgcgc
     9241 aggttacgac gcacatcttc gatcagctcg gccgcgagcg cagctcgaac gctctcgaag
     9301 cggcccggct ccggcaagat caacaacatg ctgagttgct caccgaagta gggaagctca
     9361 accgcctgca gctcaccgcg tgcgagataa cggtaacttc ccgggcgatg catcatgttc
     9421 acgttgcgcg gttccccgcc cagccccgtg aacttggcgg gatgggtgcc gccggccgag
     9481 aactcgtctt tccaattggc atccaagtag agagagctcg tcaccagcag ctgcgcctga
     9541 ggagacagcg tgtctgccga gacgagttcc tttatcttgc cgcgggtgcc gtcggagacg
     9601 aagtcattga tttcacgtgc cgtctgcttg ggctgctccg cgaaatccac caatctaacc
     9661 ccgactccgt aatttccact cagggagtcg agatagtttt tttcgaactt ggcgccgcgt
     9721 tgtgcccaca gcgcgcgcgc agaatgaatc tcgaccttct tttcggcatc tccactgcgt
     9781 acggcggcgc ggttgcgatt gacgagctcg ctcgagagac gattcatcgc cttctcgaag
     9841 gccccgcgcg ccatgcttcc gtgcagcgcc cgcccgagct cagtggccgt ctcgcctttg
     9901 gcccccgcgt acggaatggc gagcccgagc gcgatcgtga tcggcgaaca gaaggcgttg
     9961 ttgcgctctc cggagccgcg cagtatcccg agcccgaact gatttacgcc gcgcacggcc
    10021 aattccagct ctgcctggct caggccggca gggctaacac gcggactttc ccccttcact
    10081 tcgactggct tgctacaagc acaagtcagc gtggcgctcg caatcaacag cgagggaatc
    10141 aagcacgcgg ctgtattcga gaccggacca actgcgttgg gcaagcgtgt catggtgggc
    10201 tcacgagcgg gggatggtga cttttatccg aacaccagtg acgctatccg aacaccaacc
    10261 gttccagatt caccttggac cgagagctgt agttctggag ttgcgcgcct tcaccccgcg
    10321 cattgtatac gtaaacgacc ccttcgaaat ccccgaagtc gatggtggaa cttccgaagc
    10381 tgtaccattg attgagttgg caggcgacca cggtcgtgcc ggcattgacc gcgactgcat
    10441 taccgaacgt gagctggtac gagggcgaat ggttcgcctg ctccgcaccc acttggcagg
    10501 aaaagaacac gattgctccg ctgatacagc cccggagctt ctgaaatagt tgaacgttgg
    10561 accaactcaa gttgtctcgc ccgagctgga taccgccggg gaaaccatgc gccatgaagt
    10621 gcagagcgtt cagctttcct gagcctgagt tgcctttggc gacttgcgca gtccacccca
    10681 agatatgctc ggctggctct gcccccatca ccgtccaaac gttctccgcg gcgggtggcc
    10741 gcagcgacgt cggaggcgaa gtgaatgggt tattcagccg attgtcgtgc acgatcatct
    10801 gccgcccgcg gcgaaagtta taccctgcca tatctcatca ccctttcccg atacacctgg
    10861 cacaggaaaa cacgcggcgc ccagtctgtc aatcggagga agcagtctca ccgtgcgcgc
    10921 ttccaggtcg aggaatggag aaggaaagga acggggtggc gcgtgatgcg gtccgcaaag
    10981 gcatcggcga gccagtcccc gagcgctcgc atgcgaccca ccaaacgcac gccagatgcc
    11041 gtcgccaaca tcacgggata gctctcactg gccacgtccg gtagcaggtg caacaggcga
    11101 ccctgagcaa cgtcctcgca aatgtccagc caggacttga gcgtgatgcc atgacccgcg
    11161 agcgcccagc gccgcgtcac ggcaccgctg tccgtgagcc gtcgcccctt cacttttacg
    11221 tcgaccacgc gcttgccgcg caccaatcgc cagttggaag cgctgcgcgc gctcgttttt
    11281 agcagtaggc actcgtgttg attcaagtgc tcgatgcggc tcgggcggcc atgccgttcg
    11341 acgtagctgg gagcggcaac gagcacccgc cgagtttcgg ctagcttgcg tacgacgagg
    11401 ctgccctcgt gcggcgtgcc atagcgaatg gcggcgtcga agtcatcgcg ccacagatca
    11461 cgcatctgat cggagagctg aagcgcgagc tcgatccgcg agtgggtctc caggaagcga
    11521 tcgagcgcgg ggaccacgag ctgttcaccg atgtcgacgg gcgcgctcag acgaatcaaa
    11581 ccttcgactc gcgtcttgct gaattggaac agctcgcgcg cttcgttcaa cgtggtcacc
    11641 gcttggcgcg cacgctccag gtactgcttg ccctgttggg ttggcctcaa gctgcgcgtc
    11701 gtgcgatcga atagcctgcc gccgagctct tgctctaggc gcttgagcgc ggcgctggcg
    11761 gttgccggca accagccgag ctcacgggcg gccgcggaca gattcccccg ctcttccgcg
    11821 cggatgaata accggagcgt gtcgagttcc attttcaaat ctcgtttgat tattaattcg
    11881 cattctcggc attttcaaag tccgcccagc ccgatagatc cagtcgcgtg agctgttcga
    11941 gctcgcttga ccaacaggag ctacccgatg aaagccgttg ccttgacccg ctatttaccc
    12001 atctcggagc cagagtcgtt gatggacgtc gagctagaaa agccccagcc tggcccgcgt
    12061 gacttactgg tgcgcgtcca agccgtaggc gtgaacccgg tcgattgcaa ggtgcgcgcg
    12121 cccaaaccca agatcgagaa tgcacctcga gtgctcggtt gggacgccgc gggcgtggtg
    12181 gagagtgtgg gcgccgaagt cacgttgttt cgcgctggcg atgcggtgta ttacgcgggc
    12241 gacatcacgc gcccaggaac caatcaacag tttcattgcg tggacgagcg gatcgtcgga
    12301 cacaagccgc gcacgctcga ttttgcagag gccgcggccc ttccattgac cgccatcacc
    12361 gcatacgagg cgctgttcga gcgcctcgcc ctcgacatcg acggtgccga tgcaggttct
    12421 agcctgctca tcatcggggc tgcgggcgga gtgggctcga ttgccattca actggcgaag
    12481 ctcgccgggc tcagggtcgt cgcgacggcg tcgcgcgagt cgagtcgaag ttgggtcaag
    12541 tcgctgggcg ccgacgaggt catcgatcat acgcgcgagc tgcgggacga gctgacccgg
    12601 attggcgtga gtgaggtcga cgccatcgcg gtgttcagcg ataccgaccg ctacttcccc
    12661 gccctacccg ccttgatcaa agctcaaggc cgaatcacga gcatcgtgga gaccagtcgg
    12721 cacgtggatc tcgagttact caagagcaag agtctgacat tcgtgtggga gttcatgttc
    12781 acccgctcga tgcaccgaac tcccgacatg atcgagcaac accggctctt ggaccgcgtc
    12841 gccgagctcg tggattcggg gaagctgcgc agcactctga ccgagcggct ttcgccgatc
    12901 gatgcggcct ccctgcgtcg cgcccacgca gcactcgagt cgggtcacac gattggcaag
    12961 atcgcagtgg agggttggcg atagtcgaac ccagcgcgag aaccagtggc ccggcgcatc
    13021 acgaatccga ccctgccggt gcagggttct cacgagatcg ccggcccagg tggcggctct
    13081 cccggcggtg tctccggggg aggatcctcg agcggagcgg gcgcgggctc gggctcggga
    13141 tcacgcatcg gaggcggctc ttcgcgcgaa ggcgcaggca gcggcacctc cgcaggcgtt
    13201 tcggggcgcg gcacttcagc cggctcgggc gtgggactgg agggatgacc gggcggcgtt
    13261 ggcatggcca tggtgagcag agggcattgc gaaccgcatg ccggctcgat tcctcggcaa
    13321 agatcgcgca aacgcctccg tctcgcctgc gccccacgag ttcgcacgga ataccgagcg
    13381 tactcggtcg agacacgtac gcatggacgc cacgatggcg atcaggagaa aaccgcggct
    13441 gctcgagccc tcgatcggct cctcttgagt ctggccgcgc ttgagttttg ccgcgtttga
    13501 gtttgcggag agggagggat tcgaaccctc ggtacggtta cccgtacaca tgatttccag
    13561 tcatgcacct tcggccactc ggtcacctct ccgggcctgc tcggcactcg ctcgagttct
    13621 ctggagacgg cgaaccgtgc tccagtgagt cgagcgattg aaccgcgatg aggtctctcg
    13681 atcgagacct catctcgagt tcgctagcct gacgcgctta ctcagtgcgt cgcggcttgc
    13741 ggagagagtg ggattcgaac ccacggtacc cttacgggta cacctgattt cgaatcaggc
    13801 accttcggcc gctcggtcat ctctccgcgg cggaatctgg caggagcgcg ccgctctgtc
    13861 aacgcacgaa ttgaggattg aaacggttgt ttgcgtcgta tttgtcgagt cgcctatgcc
    13921 gaacggccgg ttaccgcaat cgaggcttgg agcttgcgag gccagctgtg cttcacgcaa
    13981 gaatggggtc tttcacgtcg gaaaccccgc atgagtctgt tcggtcgctt cggtctcgtc
    14041 ctgccattgg ggttgtgggt gattttggtg gcggcgttgt tccgctgggt cccgggcgtt
    14101 ccgcgcaagc gcctgcgacg cagcgtgatc ctgtacggcg cgtacgcatc gctgctgtcg
    14161 gtgagctggg tgctcacgca cttcaaagcg agcaacggct ggtccggcgc gttgctcgca
    14221 gccgccgccc tcgtagagct cttcctgatc atcaatctgg ccgccctcac gctgttcgat
    14281 tttttggcgg gttgggtgcg ggtcaagctg tcggacatcc tccacgactt ggccgtaggc
    14341 gtggcctatt tggtcgcgat cgtttggtcg atgcactcct ggggcgtgaa tttgacgggc
    14401 atcgtggcca cgagcgccgt cgccaccgcc gtgatcggtc tctcgctcca gtcgacgctc
    14461 ggcaacgtga tcggcgggtt agcgctgcag ctcgacgatt ccatcaagga gggggattgg
    14521 gtggagctcg agaacaagac tcaggggcgc gtgcagagcg tccgctggcg ccatacggtg
    14581 ctcgaaaccc gcgatcacga cacgttgatc gtgccgaaca gcatgctgct caatcaaacc
    14641 atcaaggttt tgggcaagcg cgcgggagag cccctggtgc atcgaatgtg ggtctacttc
    14701 aacgtggacc atcgcttcgc gcctggtgcc gtgatccgcg tcgtagacga agcgctgcga
    14761 gcggcttcga tcgagaacgt ggctgaagtc cctgccccgc agacgatttg ctacgacttg
    14821 gcgcgcgagc atcgagacag ctactgctac tacgccgtcc gctacttctt gaccgatctg
    14881 gcgcgggacg acccgactag ttcagaagtt cgcgagcgga tttatgcggc gctgcgccgc
    14941 gccggaatgc cgctcgcgtt gccggccgcc acgttgttcc tctcgcacga agatcaaaag
    15001 agcgaacgcc ggctcaacaa ggtgaaagcc gagattcacg cggcgctgcg cggcgtggac
    15061 ttgttctcca agctctccga agaagagctc gatattctcg ccgagtcggc caagcacatg
    15121 ccgttcgtgc gcggtgagat catcacgcgt cagggtgcca aggctcactt cctttacgtg
    15181 ctggtcaagg gcgaggtcga agtgcgagtc tcggccgaag acgaagaccg gcgcgtcgcg
    15241 gtggtcgtgg cgccgggctt cttcggtgag atggcgctga tgacgggagc gccgcgcgaa
    15301 gcaaccgtgg tgtcgctcac cgacgtggag tgcctgcgcg tggacaagga cgactttcgc
    15361 gcggtgctcg cgcgccggcc ggaaatcgcg caggagatct ccacccttct cgcgcagcgc
    15421 cgcgtggagc tctcggccgt ccgagacaac ctggacgcgg acgccaagcg tcggcggatg
    15481 caaacggagc gcggccgcat cttggcggcc atccgcgact tcttcgcact cgaagagtag
    15541 ccccggcgcc gacgtcggtg aggacgtcca gcgccggaac gctcaacgga gaagccgcgt
    15601 cattcgacgg gcgaccagcg caggcgcggc ttgcgcgcgg cgcgcgcctc gtccaagcgc
    15661 ccgagctgcg tcaagtgcgg cgccttctga acgagctcgg gcgtggtccg cgcctcgcgt
    15721 agaatcgcca ccacggcttc gacgaaggcg tcgagcgttt gtttactttc ggtctccgtc
    15781 ggttcgacca tcagcgcgcc gcgcactact agcggaaagt aaacggtggg cgggtgaaag
    15841 ccgtagtcga tcaagcgctt ggccaaatcc aaggtcttca ccgaagtctc ggcctcgagc
    15901 tcgcgatcgg tgaacacggc ttcgtgcagc gggggagtct cgaacgcgag ggggagatga
    15961 tccctcagct tggccgcgac gtagttggcg ttcagcaccg ccatgtcgct cacccgacgc
    16021 agaccctctg gcccgagttc acggatatac gtgtacgctc gcaccatcat gccgaagttg
    16081 ccgaagaacg agcgcacgcg acccacgctc ttggggcggg tgtgatcgac cacgtagcga
    16141 tcaccattac gtcgcacgac cggcgtcggc tgaaacggct cgaggtgctt cttgaatgcc
    16201 accggaccgg agccaggacc gcccccgccg tgcggagtcg tgaacgtctt gtgcagattg
    16261 aactgcatca cgtccacgcc cgcgtcaccg ggacgggccc gcccgagcac cgcattcaga
    16321 ttggctccat cgccgtacac gagcccgccg cgctcgtgga tcaaagccga caatttcggc
    16381 atcgcctgct cgtacaggcc gagcgtgttc gggttggtga tcatcaacgc cgccacgtcc
    16441 gagccggcgg cttctacggc cgcgagcagc gtctccggct ccaccacccc cgccgatcct
    16501 gcgggaaacg gcacggcctc gaagccgttc aaggcacaac tggcggggtt ggtgccgtgt
    16561 gcgctgtctg ggatgaacac cttgcgcgga gcgcgccctt ggtcggtgtg ataggcgcgg
    16621 atcatcatca gacccgtgag ctcgccctgg gcgccagcgg ccggctgcaa gctcacgcca
    16681 tccatgccca cgatctccgc gagcaacccc tcgagccgca ccatcagctc gagcgcgcct
    16741 tgaatcagag gatccggcag cagcgggtgc aagcccgcaa agcccggtag ccgcgccgcc
    16801 cactcgttga ccttcgggtt gtgcttcatc gtgcacgacc cgagtggata gaactgagtg
    16861 tcgatgctga agttcttctg actcagccgc acgaaatgcc gcaccacctc cggctcactc
    16921 acctcgggca gcccagcggg ggttcctcgc gcgaggctcc ccaagaccgc tcggctatcc
    16981 accgccggaa cgtctagctc cggcagagac accgcgctgc gcccaggtgc accacgctcg
    17041 aagatgggtg gttccgtgaa ttcgagtccc cgatcggctt ctgcgagcgc agtgtggtgc
    17101 gacatattgt gagacatctg tagcgagcgg cccttccgtg cagcgaaacc cggagcttac
    17161 ttcgagtccg agtccgagtc cttcttgtcc gacccctcct cgccgccgtc atcccaggaa
    17221 tccacgcccg tgacgcggcc ttccacctcg tccggcttca ccgacgcggg atttggcggc
    17281 atgttcttct gcttgaccgc gcgccactcg tagccggcat cggcctcgcc gaggccccac
    17341 tcacccacga tctcgtgagc ttcgccttcc ttgggtgagg tgtagcggaa gtaacctttg
    17401 cctttgcggt tcgcgtcggc gccaacggca ccgatgcggc gctcaaccca ctcgaagcgc
    17461 agcaaatctc cctcgatttg cccgtggagc tccccatagg cgtcgcccgc ggtcgtgcgc
    17521 cacgcgccgt tggccgagct gccgtcactc aacaagtgca gatagccgta aagctggctg
    17581 tagtacacgc cttgccaatt gccaccttca ggcatcgtgc ctgcggtcac gttggcgtgt
    17641 ttcacctggt tttggccgcc gccgcacgaa acgctgaaca ggagcggagc tatcagcagc
    17701 agccgggatg cgatgcggag aactcgggaa ccttgcatgc ggcgcactct acctctctac
    17761 ctggcgtcga gtcgagccag gacctcaccc aagcgttgcc ccaacccttc cgcctcgacc
    17821 tcgctgctcg gccaagcttc gcgcaatgcg tgctcacggg ctcggctgcc gtctgcctcc
    17881 gccaagaagc cgcgcaggaa catctgattg ccatcacgca tggcgaaggc cgcgaccggc
    17941 aaatggcagc ttccctcgac ggtcttgagc gtggcgcgct cggccaaaac ggcaattttc
    18001 gtgtcggggt gcgagagcgg gtcgagcaga gctgccacct ccgcgtccgc ggctcgatac
    18061 tcgatgccga gcgcgccttg gccaacagcc ggcaacatga tctcgggctc cagcacctcc
    18121 gtcacctccg ccgtgaggcc gagccgtagc aagccggccc gcgccaaaac gatggcatcc
    18181 atctcacctt cgcgacactt gcgcagtcgg gtgtccacgt tcccgcgcaa gcctacgatg
    18241 cgcagatcgg gacgcgcctt ctcgagttgc atgcgtcggc ggagcgaaga ggtgcccacc
    18301 acgctgcccg ccggcaactc caccaacttg gcccccgtgc gcgagatcag aacatccctc
    18361 ggatcctcgc gccgcgggta acagccaatc acgaggcccg acggcatctc cgccggcacg
    18421 tctttcagcg aatggaccgc cagatccgcg cgcccatcga gcagcgcctc ttcgatttcc
    18481 ttgacgaaca gacccttgcc gcccacctcg ctcaagagac ggtcttgcac gcgatctccc
    18541 gtggtggtga cggtcagctc ctcgatttcg agcttggggt gtagcgcctg aacggttttg
    18601 gcgaaggcac gcgattgcgc caacgccagc gcgctcttgc gcgtcgcgag aactagcttg
    18661 tgtttggcca ttttccgaga gtgccggtga cgtgggactt ctcgtcccct gcaatcgaat
    18721 cgccgagcgc gccgggcgtg cccgaagggc cagccgcgcg tggctgcgag acaagctcca
    18781 tttccggatc ggctctgcct tccgccaagc cgaagagctc actcagcgcc agcgacaagt
    18841 cgtcgaaaga cagcgtaccc gatttgcgat cttgagccgc ctcgcgcaaa cgcatcgtcg
    18901 gcgcgtgcag gatgcgattg gcggccgcat cgagcatggt ctccaatgct tcgcgatccg
    18961 tgagcccgag gtgcttcaag cgtccgcgcg tgctgcgctc cagctcgagc cgcagcgcgc
    19021 gccgcacctc ctcgcgcaag cgcacgatca acggcgtcgc ttgttcggcc tcggcccagc
    19081 gctcgtagcc gcgcgtctct tcatcgacga tgcgctcacc gagctcggcc tcgcgcgaac
    19141 gggcgctcaa cgtctccgcg accacccgcg aaaagtcgtc gatattgtag atgaacacgt
    19201 cgtccagctc acccccggcg ggatccacgt cgcgaggtac ggccagatcg atgaacgtct
    19261 gggcgcgacc acggcggctc ttgcgcgct
//
LOCUS       DASTKU010000463         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1873668, whole genome shotgun sequence.
ACCESSION   DASTKU010000463 DASTKU010000000
VERSION     DASTKU010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1873668"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFQ61_15980"
     CDS             <1..200
                     /locus_tag="VFQ61_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956006.1"
                     /translation="LSSVRWSRLGHYSPLQFLDCRVWEALLRFRWDLDRVEAQILATD
                     ADRFLSYPFLLPSQLGCSIYI"
     gene            215..1936
                     /locus_tag="VFQ61_15985"
     CDS             215..1936
                     /locus_tag="VFQ61_15985"
                     /inference="COORDINATES: protein motif:HMM:NF012526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoxygenase family protein"
                     /protein_id="HET9956007.1"
                     /translation="MIPCLPQKDWLPGVRKARLTADRFIYQYDRDYHDLPFPLEWPLL
                     GLFPPRYYARLAALTLAVDANQWASDALTWLNSGRVLDDFVAWMRKPERLELEYTYGE
                     REPPPRFSAGARTPESLRTYDRLLPLLPKPAAAALWCEPEFFAWQRVAGAYPIALRST
                     DSGEAWAPALTSAIDRALAPTSDCTEAARKEQRLFIVEYPLAKGLPAGFGDGWRRYLP
                     EVSGYFVRPPRQPRIQPVAIRCGDDGVFTPRDGAVWDAARAVLQAVESTVHGVAEHGV
                     ACHIVLTLIALATYRCLGHNHPLRVLLTPHFEFTIPVTLVTKSFFSPGGRTINLQSVS
                     LQGCIELARRRWGQFDWTRDTVPGSIQTRGLLAPEILPEYPFRDDVLLLEQVIRSWVL
                     AYVGLYYDDDEPLDQDHELQNWLRELSAAHCAGIAGIPKIEKVDDLVRLVTQIIHRAT
                     AYHAAINDPVYDAMGYVPNMPGACYDAPPLASASAAPDPLTLLPPRDKAQAQIADVYV
                     VSNTKLNRLGHYPACTFVDPRVWPLQRAFLDALGEAERTIEERNRTRFLPYRVLLPSR
                     LTASINV"
     gene            complement(1959..2666)
                     /locus_tag="VFQ61_15990"
     CDS             complement(1959..2666)
                     /locus_tag="VFQ61_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET9956008.1"
                     /translation="MSKDKLMLRAADLSAVELFSGLTSDALDAVLAKSAVCHFARGEL
                     ALLQGTRSGELHVVVSGAFKASSLTEGGSAVTLAIMGRNEVFGEMAFLDGGTHSANVA
                     ALIGSRTLRISASVLESLAEQHPRLRAYLAKVLTARLRRLSTASERMAVDDVTARLAR
                     QLLELSERFGAPDAGGTRIMLELPQQELAELVGATRERVNRVLAAWMKCGWVSRRGRQ
                     VVLLSRPQLSRHAGLGG"
BASE COUNT          495 a          914 c          810 g          526 t
ORIGIN      
        1 agctttcgag tgtgcgctgg agccgcttgg ggcactactc gccgctgcaa tttctggact
       61 gtcgagtctg ggaagccctg ctgcgatttc gttgggatct cgaccgtgtc gaggcgcaaa
      121 tcttggctac cgatgcggac cgctttttgt cttatccctt cctcctgccc tctcagctcg
      181 gctgcagcat ctatatctaa cttgcgagac tccgatgatt ccttgtctgc cgcagaagga
      241 ctggttgccg ggggttcgaa aggctcgact cacggccgat cgcttcattt accagtacga
      301 tcgcgactac cacgatctgc cgtttcctct ggaatggccc ctgctcggat tgttcccgcc
      361 acgttactac gctcgccttg cggcgttgac gctcgccgtg gacgccaatc agtgggcctc
      421 ggacgcgcta acctggttga actcggggcg cgtactcgac gatttcgtgg cctggatgcg
      481 caaacccgag cggctggagc tcgagtatac ctatggcgag cgtgagccac caccacgctt
      541 cagcgccggc gcgcgcacac cagaatcgct ccgcacatac gatcgactgc tgcccctgct
      601 gccgaaaccg gccgcggccg cgctctggtg tgaaccggaa ttctttgcat ggcagcgtgt
      661 cgcgggagcc tatccaatcg cgctgcgttc caccgattcc ggcgaggcat gggcgcccgc
      721 attgacatcc gccatcgacc gcgcgctcgc gccgacctcc gactgcaccg aagcggcacg
      781 aaaagagcaa cgcctgttca tcgtcgaata tccattggcc aaggggctgc cggcgggctt
      841 cggcgacggg tggcgccgat acttgcccga ggtgagcggc tacttcgtgc gtccgccgcg
      901 gcagccgcgc atccaacccg tcgcaattcg ctgcggtgat gacggggtgt tcaccccgcg
      961 agatggcgcg gtgtgggatg ccgcgagggc cgtcttgcaa gcggtcgaga gtacggtgca
     1021 cggcgtggcg gaacacggtg tggcttgcca catcgtgctt acattgatcg cgctcgccac
     1081 gtatcgctgc ctcggacaca atcatccgct gcgcgtactg ctcacacctc atttcgaatt
     1141 tacgatccct gtgactctgg tcaccaagtc cttcttcagc ccggggggca gaaccataaa
     1201 cctgcagtct gtcagcttac aaggctgcat cgaactcgcg cgtaggcgct ggggccaatt
     1261 cgactggacc cgagacacgg tccccggatc catccaaaca cgcggcctgc tggctccaga
     1321 gatcttgccg gagtatccgt ttcgtgacga tgtgctgctg ctcgaacagg tgattcgaag
     1381 ttgggtcctt gcttacgttg gcctgtacta cgacgatgac gagcccctcg accaggatca
     1441 cgagttgcaa aattggctac gggagttgag tgccgcgcat tgcgcaggaa tcgctggaat
     1501 tccgaagatc gagaaggtgg acgatctggt acggctcgtc actcagatca ttcaccgggc
     1561 cactgcatac catgccgcga tcaacgatcc tgtctatgat gcaatgggct acgtaccgaa
     1621 tatgcccggc gcatgctacg acgcgcctcc gctcgcttcc gcgagcgcgg ccccagatcc
     1681 cttgacgctg cttcctcccc gagacaaagc ccaagcccag attgccgacg tgtacgtggt
     1741 ctccaacacc aagctcaatc gactcgggca ctacccggcc tgcacattcg tcgatccgcg
     1801 cgtgtggcca ctccagcgag cttttttgga cgcgctaggg gaagcggagc gcaccatcga
     1861 ggagcgcaac cgaactcgct tcctgcccta ccgcgtactg ctgccctcgc gtttgaccgc
     1921 gagcatcaac gtgtgagcgg aaaagccaac cctgcgcgct agccgcccaa acctgcgtgg
     1981 cggctcaatt gtggacgcga cagcagcacc acctggcgcc cccggcggga tacccagccg
     2041 cacttcatcc atgccgccaa tacgcggttg actcgctcgc gcgtagcgcc caccaactcg
     2101 gccagctctt gctgcggcag ctcgagcata atccgtgtgc cacccgcatc gggagcgccg
     2161 aagcgttccg agagctcgag cagctgacgg gcgaggcgcg ccgtcacgtc gtccacggcc
     2221 atgcgctccg aagccgtact cagccgacgc agccgcgccg tgagcacttt ggcaaggtat
     2281 gcacgaaggc gagggtgctg ctccgccagg gattcgagca cgctcgcgct gatgcgcagc
     2341 gtcctcgacc ctatgagggc ggcgacgttc gcggaatgcg ttccgccgtc cagaaaggcc
     2401 atctccccga acacctcgtt gcggcccatg attgccaacg tcaccgcgct tcccccctcg
     2461 gtcagactgg atgccttgaa ggcgccagag actacgacgt gaagttcacc cgagcgggtg
     2521 ccctgcagca gcgccaactc cccgcgcgcg aagtgacaca ctgcggattt ggccaacacg
     2581 gcgtcgagcg cgtcgctcgt cagcccggag aatagctcga ccgcggagag atcggccgca
     2641 cgcaacatga gcttgtcctt tgacataccc taccgagcca ccactctatc gtggctcgaa
     2701 tgggggtcaa tgagcgcgcg tgtttcaacg gcggtttcga tgcgc
//
LOCUS       DASTKU010000464        19624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_311650, whole genome shotgun sequence.
ACCESSION   DASTKU010000464 DASTKU010000000
VERSION     DASTKU010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 19624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19624
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311650"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFQ61_15995"
     CDS             <1..451
                     /locus_tag="VFQ61_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956009.1"
                     /translation="YRSFWTPALTDELLLTPQGQTNLAHPLMEALEWGAELILIVSDG
                     HENAPPHGAAELARVFRKRLDPKGRTSIIHLNPVFDAEQYAPHAMGAAIPTLGLRDAE
                     DLLTMLGFARFADGSAPLSELETYLEARAQRLIANEHSGARDLTITT"
     gene            460..1626
                     /locus_tag="VFQ61_16000"
     CDS             460..1626
                     /locus_tag="VFQ61_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956010.1"
                     /translation="MAKNHSLLQRLQLRGLELAPPQVWGGIRLVPLLRREVREDLRLF
                     RRTYDEPVGVVGLEGDALGPGLKYVSYIPHGLIVSWSDDGAPCAAFGAQLMADGKRLG
                     GSAGGVRVLHRMARRESANRLRLLPLHLAMEGFLALHFGGPELAWTEYSNYALSRGLD
                     PRSELTFAGMWIPGLEAALRVFEIHENQAGLILFIGESLASIFVVPHPSDYRALHRTL
                     LEDFFGEQLLYHGLYAAPAQVTASMDDSRVGSLLDLRRELEILNAGWRDFHALLAAGL
                     LGVAIHSERVYRAGPFQLQRFSTPLDPATENHIGEAIVRDSGALEYMKTYRLSAAQTR
                     RAYLLSQLAAHDWHLERTAGALRQTKEELVQRLDKAGFGYLLRDHVLEAARRRR"
     gene            complement(1694..2263)
                     /locus_tag="VFQ61_16005"
     CDS             complement(1694..2263)
                     /locus_tag="VFQ61_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956011.1"
                     /translation="MSFRLFGAAVGSRTLGLALFEARLSRRLWGFVEPTFGHYRERNE
                     YNVDNPNIQYPKGNSTSLGVDVGVRWIANPGGAVEVGLYHGLRHQWAWSTRQERQIEF
                     IEAGNPQFGNAETSSKTYGAEFATGLVVERELIPHLWLRIQSSLLSARYEKTRFERTT
                     SEPRSTFTSGESLDLGIRINPMLKLRVSW"
     gene            complement(2759..3607)
                     /locus_tag="VFQ61_16010"
     CDS             complement(2759..3607)
                     /locus_tag="VFQ61_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956012.1"
                     /translation="MPPSTTVLTDANFQTVRNWSVELVNSTPEWKLRAALTSRTPGQV
                     LGLVCDEQLAPTGCDPGQARCRLGCEAIRICETGGCAGGHWVNFEVDTVGKALFVSDE
                     QTTEMLPYGKWRNRFRATAIESGEPEFRALRAPRQTFPDHQPGHGEFGYGKPTNTSAD
                     TLPSMRDVFVSGHYRLLRTETYGKPGFPLFVVEPERTAAHESTAAHESTAAHESAEEA
                     EFRKWGAALVRANAQRPCEVVILDSNGSNRRFQFDRFALASGHGGVYILIESEFQPWL
                     GGVHPQ"
     gene            complement(3832..4767)
                     /locus_tag="VFQ61_16015"
     CDS             complement(3832..4767)
                     /locus_tag="VFQ61_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9956013.1"
                     /translation="MATLKQLNGSKSVVLEAASVVGRARGSTLLISEPHVSNQHTLLR
                     WKSNHWELRDRSHNGTYVNGVRLPTDAPRTLVKGDVLAFGDVSREWVFIDDSPPCVML
                     VATDGTTLIAEDDLLGVPSPEHPELTIFCDSDGVWKLEANDGATSVLENGTEFTCAGQ
                     KWRFCCPQEDESTLTAEHIPSSRQNQAEASPVLHFLVSADEEYVELRLEYDSNSKVVH
                     LGARNLNYLLLVLARARIEDMSRGLPDTSCGWMYKEDLATSLHMTPTQIDGEVFRIRH
                     HFARFKLKEAALVIERRGRTGQLRIGLRRLRITKL"
     gene            complement(4836..5918)
                     /locus_tag="VFQ61_16020"
     CDS             complement(4836..5918)
                     /locus_tag="VFQ61_16020"
                     /inference="COORDINATES: protein motif:HMM:NF019429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-like protein"
                     /protein_id="HET9956014.1"
                     /translation="MSIFLFCGPSLPPETAASLLPAAHCEGPAQCGDVYRAVRRGATT
                     IAICDGYFEHAVPVWHKEILWALSRGVRVYGAASMGALRAAELSPFGMRGVGRIFEQF
                     RDGVLHDDDEVAIIHESPEQGYRVRSDAMVNIRATLEVATASGAIPSSFAQRLVAIAK
                     ALFYPERSFSRLLENAAREGQALAHIEALREWIATHSVVDQKGLDARALLQELRSELD
                     EPSPMLEPRVPFVFQYTDAFHALRQHLEATERANDGSRAPDPLERQVRAEALERALAL
                     SLADMDGFEPQAEDIQLLSELFREERGLLDSEQTSAWLSARNLDVEDFSRLIYESLLV
                     ERYRADARRLAALQFDTARLLMAPVR"
     gene            complement(5915..7147)
                     /locus_tag="VFQ61_16025"
     CDS             complement(5915..7147)
                     /locus_tag="VFQ61_16025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HET9956015.1"
                     /translation="MSVLSHRTRKLRFAGTLRGRSPSETLAAIESLPSRVGITRVANV
                     TGLDCIGIPNYVAIRPNSRSLSVSQGKGVDHDMARLSALMESIEQHFAETADVPLRRG
                     TYAELRSRYHVVDPFSLPAYARRWTEHTPMLWTTAHEIASGAPVEVPFDLVHANLTLP
                     LPSDSGYFPIGTNGLASGNSVAEALVHGLCELIERDSLALFYASPPADQFSRCLALDS
                     VADPVASWLLERYQLAGIRVAVWDITSDIGVSAFFCSIIDETPDPFRQVGLASGFGCH
                     LDRSVALCRALCEAAQSRLTKITGSRDDISFRDFARNGSHEQMRRARAQLPSAGFREL
                     GSIPNIDFETFEDALGVLGAALTRCGLGPASFVVLSPADAPVSVVRVIVPGLEGVPTV
                     PGYRAGSRVRGLGRACAS"
     gene            7301..8077
                     /locus_tag="VFQ61_16030"
     CDS             7301..8077
                     /locus_tag="VFQ61_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956016.1"
                     /translation="MQSRQPRPRPPSRAELPQVVDPKDKLDHDVLVFPRTGVVEEWQY
                     FRIPVVKQARELSDGTTIRAGQFVSAPEKWDLSDHGERRVLSTARPQHYGNEDERSAA
                     RGPAFALLPVKPDPSSASFPTCFLINVQNLDSPNLWTVEEWNDGPGGPDYPTPQHPNE
                     FEALVAGPRGAVFRLKITNVDAWRPGTQPLPNGLGEIEAVDLANESEIWGQLRNGCIA
                     GRALFQDTSTNKRENKHRPQKRVIPLVNITALQPGLSTKV"
     gene            8081..9205
                     /locus_tag="VFQ61_16035"
     CDS             8081..9205
                     /locus_tag="VFQ61_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956017.1"
                     /translation="MSCCTSSQRRSPGMGYRDPITNKDRVEVMGTWDFKWPLGSKIRV
                     AFQQPSSREINEADFLESVQIIKQLAELWIKAAPHQAAPNIEFDWSPPPFSRADPTDP
                     QNGSTFAFPSGKQEYRARDYDVLISLDTLPRGKRDPATLERVQRNFLPSSELGSFARR
                     VDYGVPTAYLGPFAGQASADGEFRLAHYYKTNSIARFYVIHEFGHILGLAHTHQSRSR
                     RATLPPEAVARLTNIVIHSQNLIRVESSAARWGDTGLEASTSIYSGQRRELRKEIAPI
                     KATLRAFLDRRHIPITLEEAELDDFVVSQIVESWPGNDAFSDWPAAGPSIMDIPYFEC
                     LIGLHRAADGFCEICEELISRGAPLEGDLRALAAMYPCVE"
     gene            complement(9219..12605)
                     /locus_tag="VFQ61_16040"
     CDS             complement(9219..12605)
                     /locus_tag="VFQ61_16040"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9956018.1"
                     /translation="MQAVIGRYELGALLGEGGTGSVYRCCHTETGVRACLKLPNEDSS
                     RRREPLRREIAALKRLNRYQHPGVVRLIDSGEHAGAPFYVMEHIQGEPLTRLAEKAAW
                     LASSGEEGSRRVQLNEVLELAAQLADALELVHSEGVVHGDLSPANVIVRDGGQPVLVD
                     FGACFQAHERGLPREVAQPSGPVHGTLAYLAPERLDGGPLDARCDLYSLGCILYELIA
                     GRRPFGGARAIDLMMQHRVIVPARLSQQLPWVPSELDRLVARLLAKEPSDRIGRARDV
                     ADSLRRLVPAPRPFPPSDYPITLYRSRFVGREELLDQLTQLVNDAERGSGGLALVSGE
                     SGIGKTRLLNEVARRALERGLSVVSCPSSQYSFDTRGPSLVLPELAPFAPLLQHLNDE
                     CSQRSPAEPRMREALQDIASYVGGTRSRTELSPGKRAPADVVRRRVLSGLRDALLAYS
                     NQRVLLILVDDLQWMDELSIQFLVEHGREFCRSHILIFGVYRKEQEPEALTLLRRAAI
                     SHVNLPRLGQSTIHVMAKDMLGLTSMPEGLSQFLFERSEGNPFFASEHLRAALASGQL
                     RSNGDNDWAFEPGLAPHGLPSSLDDLLALRVAKLTPSAVATLQMAAILGREFDAELLQ
                     SLRDTQSRSTATALEELVARQILEWVKAGRYRFAHDGIRESAERSLHEVTRRSLHRRV
                     ALRLEELNAVSARPEWIAQLGIHWANAAEPERALPHLRDAARAAAESSAKLRAAELYE
                     LAVAECRCLVGQRQTRYRPTLAELLESLADTWLSQAQHHRAREAYSESLELWRSLPGA
                     PLHTARLHRKIAASYWTLHDYENARLNLDTAREMLGNPVTAADFREYIEIEIGRLEQL
                     YFSRQVGDDTERLIRQLEPIVEMHSSAEQRAYFCVGAACELAARGRYALNPESLRLAR
                     LALSHGAQAMATNQIALARLTLGFLLLPGSESDCREGVDVLRQAERDAITLGDSTLLS
                     RVLTYSMLTHVRLRDRRQAAQVAERAVVIAEEVQLLPYVGAALACQGWSRWVSGESFH
                     ARPLLEKALAVWQAHPHPFPFMWIALFPLIDIQLQQPGGHHLLREYLAALLAPTQQAL
                     PSALHSAIAAALAEDESASPARFDRAVAQALDCAHGARFS"
     gene            complement(12922..14139)
                     /locus_tag="VFQ61_16045"
     CDS             complement(12922..14139)
                     /locus_tag="VFQ61_16045"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET9956019.1"
                     /translation="MNFTASLALAALFAALGCSQQFADPIGGAPIDTNDPCSAWTTEE
                     QCRSDAQHGCSVQPNPIGCRSSDASCPASSCISGDPFVRRTGTTLSLASQPYRFVGAN
                     SWGAAWAAGGCRISEFPDAESAVTRVFDDLVELNARVLRVWAFQSYAGVTGADYSAFE
                     RLVSHAKRAGVRLIFVLENQYSDCTRGARTDDWFRSGYAMSYGGYTLSYPDYVNQLVQ
                     HFRDEPAILAWELLHEGGSDDFAAMDDFVERMSTLIRVADPNHLIAIGVNNGYSTGTS
                     NQGVNSSYAELHAHPTVDLVDVHDFDAPNESITEQVATNSSIAAALGKPILLGAIGVK
                     LEDPSAAAFERRAASVRAKLTTAYERGFVGALLYDYYPDWRTPSYNFDSRSGDPLGGN
                     QGVLAEFARQFQK"
     gene            complement(14136..15170)
                     /locus_tag="VFQ61_16050"
     CDS             complement(14136..15170)
                     /locus_tag="VFQ61_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956020.1"
                     /translation="MALLALSMGVVCEPAVSCAGPQDAEAKPSTDSETLAVLILDTED
                     SPLARRLRQELEALGFEVDVRKARTTSLSDELREPNTIAAIEILPTSSGQINLVVKAP
                     KSSKLIRHSLPIEAQQDPAAAELVTTRTVELLRAARLEYEQVRQRSTHSEPLRQRQEL
                     QPAPARPVSAAKHERLGPAFAAGGSVLTAPPFSPGFGGWFLVAWRTGDHWALAAEASL
                     PLSGSTLQRTEGEVEARSSSYKLGVWLEPSREARLAIGGSAGAQLERTSFSGAARAPY
                     TNAEAALWTWGPWLRGTTALELTTNLRVLAWATLGLSLPKTTVRVASKEITDFGRPSL
                     NLGVGLEWSP"
     gene            complement(15226..16542)
                     /locus_tag="VFQ61_16055"
     CDS             complement(15226..16542)
                     /locus_tag="VFQ61_16055"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9956021.1"
                     /translation="MAKPVGPVERIGRDVAHALEQHSAHRQKAIERARRAFLEKHVGL
                     ASDPGIARRADTRRVETRRWLGWASAAALVLCSGLAVMVWTRDERALSFAVNGQHGRV
                     DTWLGAKVKPVVLTFSDGTALQLEHASQARVVEVTPHGARIALESGILRAEVVHTEHS
                     TWQLVAGPLMVVVTGTRFEMKWNPSLEEFTILVSEGSVRVSGSVAGAGRAVRAGEKLF
                     VSVREDRLELTGSSAKPTLSAVPIPPAPPAPVPEAPIQAQPLSQQAARPRSELSQHLA
                     KPSIAPKNAVPDWQALLGSGELRAAFANAEQTGFQKVCETASAGELLGLGDAARVSGR
                     PDRASEALLTLRRRFPGDTRSAAAAFALGKVAFDQRHTYAEAASWFATYLREQPRGSL
                     AREASGRYMEALRATGDRVAAGKAARDYLARYPDGAHAELARSLLP"
     gene            complement(16527..17189)
                     /locus_tag="VFQ61_16060"
     CDS             complement(16527..17189)
                     /locus_tag="VFQ61_16060"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9956022.1"
                     /translation="MPSSQRANYSRPSASPVVTLPVGFAGDDIALVAAIREGRPAAKA
                     EFFNRYVRDVERMLTHILGPDRELADVLQETYTRAFASLPSLKNPSALKPWLFGVASR
                     TAQKLLRARARRAWLRYFVDDAEEARYEPVTTGLDPQTLHAVHAVYGILDQLPTDQRV
                     AFSLRFIDSMELTEMAEVLGVSLSTAKRRIARAQARFVAAASKNRILSEWLERGGRWP
                     SP"
     gene            17473..18039
                     /locus_tag="VFQ61_16065"
     CDS             17473..18039
                     /locus_tag="VFQ61_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956023.1"
                     /translation="MKRSSLVLAAVAVMAGVVWLAWREVRPTPREAAPLAAEEEAGTP
                     APPPLSASAAPTSPSTFREATTPISASAGPRDALPSQPLPVQPTDAELMNSLHELGWS
                     NPTKTLELARQGNSRFPSSANAPERASLIIRALSSLGRQEEARVEAKKMLETYPRSSW
                     TEDVHKHMFVNPPTHPAEHGYGKEFELE"
     gene            complement(18111..>19624)
                     /locus_tag="VFQ61_16070"
     CDS             complement(18111..>19624)
                     /locus_tag="VFQ61_16070"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET9956024.1"
                     /translation="RRIDAAVARRAEFLPSTHPEKFMQSTNQTPPLVSDCAAERNFPT
                     QLVLRRARLGLLIGALALSGCSGSEGVPSSESGGSLGEPAGGKTSSGGAVTSGGSVST
                     GGASAGGVLSNGGSTGSPGQTDTLATGGATASGGTPNGGSTAQGGGSQGGTHSGGTAP
                     GGAGGSSTGGTANGGSGAGGTSGGTTNVGGTGGGGGTSNCTVTPIDPKATQATKNLLC
                     YLYSIYTKSVLSGQQEQSWDPDRNALFTYTNQQTGKYPAVWGGDFLYEGSATYAKPYA
                     AANGIIMMRYHMGAPPNADTYENSKLTVTNYDNLTVAGTAENTSLNSKLDRMATQLKD
                     MGMPIILVPYHEVDQFAWFWWSKGTGPQFQKLWKYTVDYINNVKDVHNVIWTLGFGHD
                     GALAAYYPGKEYVDLGGIDQYDKGTQPFLTQWNSVKSVFGNTMPLPLHETGTLPQPAD
                     MWAKGATWLLWNVWCNYLNTAAEGYTWNTPETIKSAYADSHTITRDELPTNLK"
BASE COUNT         3666 a         6224 c         6040 g         3694 t
ORIGIN      
        1 gtatcggtct ttctggactc ctgctttgac ggacgagctc ctcctcactc ctcaggggca
       61 gaccaatttg gctcatcccc tgatggaagc gttggaatgg ggcgcagagc tgatcctgat
      121 tgtctcggac ggtcacgaga acgctccccc gcacggtgcc gccgagctgg cgcgtgtttt
      181 tcgaaaacgc ctcgacccca aagggcgcac atccatcatt cacctcaatc ccgtcttcga
      241 cgctgagcaa tacgcgcccc acgccatggg cgccgcgatc ccaacgctcg ggttacgtga
      301 cgcagaggac cttctcacga tgcttgggtt cgctcgtttt gccgacggct ccgccccgct
      361 ttcggaactc gagacctacc tagaggcccg cgcgcagcgg ctcattgcca acgaacactc
      421 gggtgcgcgc gacctgacca tcaccaccta gaaactccga tggcgaagaa ccattcactg
      481 cttcagaggc tccaactgcg tgggctcgag cttgccccac cccaagtttg gggtggaatc
      541 cggctcgtgc ctttattgcg acgggaagtc cgcgaagact tacgcttgtt tcggcgcact
      601 tacgacgagc cagtcggagt cgtgggtctc gaaggggacg cactggggcc cgggctcaag
      661 tacgtctcgt atatccctca tggtttgatc gtcagctggt cggatgacgg cgcgccctgc
      721 gctgccttcg gcgctcagct catggccgac ggtaagcgtc taggtgggag cgccggcggc
      781 gttcgcgtgt tgcaccgcat ggcgcgtcgc gaatccgcca atagacttcg cctcttgcct
      841 cttcatttgg cgatggaggg ctttcttgcc ttgcactttg gcggcccaga gttggcgtgg
      901 accgaatatt cgaattacgc tctctcgcgc gggctcgatc cgcgatcgga gcttacattc
      961 gcgggaatgt ggattccggg gctcgaggcg gcgctgcgcg tgttcgaaat ccacgagaac
     1021 caggccggac tgatattgtt cattggtgag tctctcgctt cgatcttcgt ggtgccacat
     1081 ccgagcgact atcgggctct ccatcggacc ctcctggagg acttctttgg tgaacagctc
     1141 ttgtatcatg gactttatgc cgcccccgcc caggtgacag cctctatgga tgatagccga
     1201 gttggctctc tcttggacct ccggcgcgag ctcgagatat tgaacgcggg ttggcgagac
     1261 tttcatgcgc tcctggccgc cgggcttctc ggggttgcca tccactccga gcgtgtttac
     1321 cgcgcgggcc cttttcagct tcagcgcttc tcgaccccgc tcgatcccgc gaccgagaac
     1381 cacattggag aagccatcgt gcgcgacagt ggagccctcg agtacatgaa gacgtatcgt
     1441 ctctcggccg cgcaaacgcg ccgggcatac cttctctccc aactggccgc gcacgactgg
     1501 catctcgagc ggaccgcggg ggccctgcga cagacgaaag aggagctcgt gcagcgcttg
     1561 gacaaggccg gattcggtta cctactgcgc gatcacgtgc tcgaggccgc acgtcgacgg
     1621 cgctgagctg acccgatggg ccggagctcg cgcgagacgt acctgcccct cacgtgcgtt
     1681 cgggcgctcc ccatcaccaa ctcacacgta atttcagcat cggattgatg cgaattccca
     1741 agtcgaggct ctcacccgag gtaaacgtgg agcggggttc ggaggtcgtg cgctcgaagc
     1801 gcgttttctc gtaacgggca ctgagcaacg agctctgaat gcgcagccag aggtgaggga
     1861 tcagctcgcg ttctacgacc agcccggtcg cgaactcggc cccataggtt ttgctcgagg
     1921 tttctgcatt cccgaactgg ggattgcctg cctctatgaa ctcgatttgc ctttcctgac
     1981 gcgtcgacca cgcccactgg tgtctgagac cgtggtacaa accgacctcg accgcaccac
     2041 ccggatttgc aatccaccgc acgcccacgt ctacgcccag cgacgtggaa ttgcccttgg
     2101 ggtactgaat gttggggttg tcgacgttat attcattccg ctcgcggtag tgaccgaaag
     2161 taggctcgac aaaaccccag aggcgccgtg agagccgtgc ctcgaagagc gcgagcccga
     2221 gggtgcggct cccgacggcc gctccgaaca gccgaaaact catcacttcc tgaaccgaca
     2281 cccctgctcc gacggcccag cgatctgtat cttgcaccag gggcggtggg accggcgatg
     2341 gtaaccccat cggtgaagcc ggcggtacaa cgagcacggg tgtgtagagc ggaaccggcg
     2401 cgggcatcac gtagtacggg gttggggcag tgcccgcagg agcttgtgct ggccaaggcg
     2461 gaggaggaac cacaggaggg ggcccacccg gcccgggtga gagggcaggc gcggttggtg
     2521 gccttccttc caggttttgc gcctgggcag accgcaccgt tacagcgcac aacgcgagga
     2581 ggagccaagt tccacaccta cgatagacac gagcgatcat cttgattcct tggacgtaac
     2641 ccagaactcg ctaccgagtt cgcattcaaa cgcggactat caccaagact ctattgggaa
     2701 tcaagtgcgg cgtcacgacg agcgggcgct cgctcactag cgcaagcctc gttgcggact
     2761 actgcggatg aacgccgccg agccacggtt gaaactcact ctctatcagt atgtaaacgc
     2821 caccatggcc cgatgcgaga gcaaagcggt cgaactggaa gcggcggttc gaaccgttgc
     2881 tgtccaaaat cacgacttcg cacgggcgct gcgcgttggc tcgtacgagg gccgcacccc
     2941 acttgcgaaa ctcagcctct tccgcgctct cgtgtgcggc cgtgctttcg tgtgcggccg
     3001 tgctttcgtg tgcggccgtg cgctcgggct cgaccacgaa caacgggaag cctggcttcc
     3061 cgtatgtttc ggtgcgcagc aatcggtagt gtccactcac gaatacatcg cgcatggatg
     3121 gcaacgtgtc tgccgacgtg ttcgtaggct tgccatagcc aaactcgccg tgtccaggtt
     3181 gatgatcggg gaatgtttgt cgcggggccc tgagcgcccg aaactccggt tccccgcttt
     3241 cgattgccgt cgcacggaag cgattgcgcc acttgccgta agggagcatc tccgtcgttt
     3301 gctcgtcgct gacgaacaac gcttttccta ctgtatccac ctcgaagttc acccagtgcc
     3361 ctccggcgca gccgccggtc tcgcaaatgc ggatggcttc gcagccgagt cggcacctcg
     3421 cttggcccgg atcacagccg gtgggggcaa gctgctcgtc gcaaaccaag ccgagcactt
     3481 gccccggcgt gcgagacgtg agcgccgcgc gcagtttcca ctcgggggtc gagttcacca
     3541 gctccacact ccaattccgc actgtctgaa aattggcatc ggtcaggacg gtggtgctgg
     3601 ggggcacggc cgtgtcagcg gtggttggcg ccgctggatt ctcgcccgag ccgcgctcca
     3661 ccgttggtga agcgggggag ggttcggacc tccgtgtgga cggacggccg cagcaaaccg
     3721 cggtgctcgc gagcgcaaag gcgatcaggg agcttaacct cgtttggctc ctaaccatcg
     3781 gtggatttcg cactctccat gactgactcc cgccttcaaa aaaacttcag gttagagttt
     3841 ggtaatccgg agtcgacgca agccgatgcg gagctgaccg gtgcggccgc gtcgctcgat
     3901 gaccagcgcg gcttccttca acttgaaccg ggcaaaatga tggcgaattc ggaatacttc
     3961 cccgtcaatt tgcgtgggtg tcatatggag ggacgtcgcg agatcttcct tgtacatcca
     4021 gccacaagaa gtgtcaggta ggcctcggga catatcttcg atgcgagcgc gcgcaagcac
     4081 gagcagcaag tagttgaggt tgcgcgctcc gaggtgaact accttcgagt tcgagtcgta
     4141 ttcgaggcga agttcgacgt attcctcatc cgcagagacg aggaaatgga gaacaggcga
     4201 tgcctcggcc tggttctgcc tcgatgaggg gatatgctcc gccgtcagcg tggattcatc
     4261 ttcctgcggg cagcagaagc gccacttctg tccggcgcac gtgaactcgg tcccgttttc
     4321 caagaccgag gtggctccgt cgttggcttc tagcttccac actccgtccg agtcgcagaa
     4381 gatggtcaac tccgggtgtt cgggtgaagg gactccgaga agatcatcct ctgcaatcag
     4441 ggtcgttccg tcagtggcaa ccagcatgac acacggcgga ctgtcgtcga tgaataccca
     4501 ctcccgggag acgtctccaa aagccagcac atcgcctttg acgagggtgc gaggcgcgtc
     4561 cgtggggagc cgaacgccgt tgacgtaagt gccgttgtga cttcgatcgc gtagctccca
     4621 gtggttcgac ttccagcgca ggagggtgtg ttggttcgag acatgaggct cggaaatgag
     4681 gagcgtggag ccacgcgctc ggccgacgac cgacgcggct tcgagcacta ccgatttcga
     4741 gccgttcagc tgcttcaggg ttgccacggg cctgttgcga gttctacatc agcatcgtgt
     4801 cgggcgcgaa ccctgtggtt ccccgcgccc cccgattacc ggacgggagc catcagcagc
     4861 cgggccgtgt cgaattgtaa tgcggccaac cggcgcgcgt cggctctgta acgctcgacc
     4921 aacaggcttt cataaatgag gcgactgaag tcctccacgt cgaggtttct ggcggatagc
     4981 caggcggagg tctgctcgga gtctagcaac ccgcgttcct cccgaaagag ttcggaaagc
     5041 aactggatat cttcggcctg gggctcgaag ccatccatgt ccgcgagtga tagggccaga
     5101 gctcgttcca gtgcctcggc tctgacctgt cgctcgagtg gatccggggc acgcgagcca
     5161 tcattcgccc tctccgtggc ctcgaggtgc tgcctcagtg cgtgaaaagc gtccgtgtat
     5221 tggaacacga agggaacccg aggctccagc atcggactgg gttcatccag ctcgcttcgc
     5281 aattcttgca gcagcgcgcg tgcgtccagc cctttctggt cgacgacgga gtgcgtcgcg
     5341 atccattccc tcagcgcttc gatatgggcc agggcttggc cctctcgggc agcgttctcg
     5401 agcagacgag agaagctccg ctccggataa aagagggctt tggcgatcgc cacgagccgc
     5461 tgcgcaaagg aactcggaat cgcccccgac gcggtcgcaa cctccagcgt tgcgcgaatg
     5521 ttcaccatcg cgtcggagcg aactcgataa ccctgctccg gggactcgtg gatgatcgcg
     5581 acctcgtcat catcgtgcaa gaccccgtcc cggaactgct cgaaaatccg gcccaccccg
     5641 cgcattccaa acgggctcag ctcagccgcg cgcagcgcgc ccatgctcgc cgcaccatag
     5701 acgcgaacgc cgcgcgacaa tgcccacaag atctccttgt gccacacggg cactgcgtgt
     5761 tcgaagtatc cgtcgcagat ggcgatcgtc gtcgcgccac gccgcacggc ccgatacaca
     5821 tcgccacatt gcgcgggtcc ctcgcagtga gctgctggca gcaacgaagc cgcggtctcg
     5881 ggcggcagac tcggcccaca aaatagaaaa atgctcatga tgcacaggcc cttcctagcc
     5941 cgcggaccct cgacccggcg cgatatcccg gcacggtcgg cacaccctcc aaaccgggta
     6001 cgatgacgcg tacgaccgac acgggcgcgt ccgctggcga caacactacg aacgacgctg
     6061 gtccaaggcc gcagcgtgta agcgccgcgc cgagcacacc caacgcatcc tcgaatgtct
     6121 cgaaatcgat attggggatg ctgccgagct cgcgaaaacc agcgctgggg agctgcgccc
     6181 gggcacggcg catctgttcg tgtgagccat tgcgagcgaa atcgcggaac gaaatgtcgt
     6241 cgcgcgagcc ggtaatcttc gtaagccggc tctgcgccgc ttcgcacagg gcgcggcaaa
     6301 gagcaacgga tcgatcgaga tggcagccga accccgaagc caagcccact tggcgaaacg
     6361 gatcgggggt ctcatcgata atcgagcaaa agaaggccga gactccgata tccgaggtga
     6421 tgtcccaaac agcgacgcgg atcccggcga gctggtaacg ctccaggagc caggatgcca
     6481 ccggatccgc gacgctatcc agcgctaggc agcgcgagaa ctgatcggcg gggggcgatg
     6541 catagaacaa cgccagtgaa tccctttcaa tgagctcaca caatccgtgc accagcgcct
     6601 cggctacgga gttgccggaa gccaagccat tcgtcccgat gggaaaatac ccagagtcag
     6661 agggcagcgg caaagtaaga ttagcgtgca ccaggtcgaa cgggacctcc acgggcgctc
     6721 cgctcgcgat ctcgtgggcc gttgtccaca gcatgggcgt atgttcggtc cagcggcgtg
     6781 cgtaggccgg caaagagaac ggatcgacga cgtggtagcg cgaacgtagc tctgcatagg
     6841 tgccccggcg tagcggcaca tcggcagttt cggcaaagtg ttgttcgata ctctccatca
     6901 acgccgagag ccgggccatg tcgtggtcca cgcccttgcc ctgactgacg ctgagagagc
     6961 gggagttggg tcgaatcgcg acatagttgg gaattccaat gcaatcgagc cccgttacgt
     7021 tggctacgcg agtgatcccg actcggcttg gcagactctc gatcgcggcg agtgtttccg
     7081 atggcgatcg gccgcgcaga gtgcccgcaa accgcaactt tcgagtccgg tgggacagaa
     7141 cgctcattgt cttagagtgc gcccgagtat cggctcgacg caaggctgtc tgcactgccc
     7201 ggcaaactta ccatagctcc tcgaaaccga agcttctacc ttggagtgcg gcgcgaactc
     7261 gtgggtctcg agtgagcgtc tcaactggga gtgtacaggc atgcaatctc gtcagccacg
     7321 gccgcgaccc ccgtcgcgcg cggagctacc ccaagtggtg gatccaaagg ataaactcga
     7381 tcacgacgta ctcgtgtttc cgcgcacggg agtggtcgaa gaatggcagt acttccgaat
     7441 ccccgtcgtc aagcaggcta gagagctctc ggacggaacg acaattcggg cgggacaatt
     7501 cgtgtcagcg ccggagaagt gggacttatc cgatcatggt gagcggcgcg ttctgtcgac
     7561 cgctcgtccg cagcactacg gaaacgagga cgagcgctcc gccgctcggg gccccgcctt
     7621 tgcgctgctt cccgtcaaac cggacccgag ttcggcttct ttcccaacct gctttctgat
     7681 caacgttcag aacctcgact ccccgaatct ttggaccgtc gaagagtgga atgacggacc
     7741 gggagggcct gactatccta cacctcagca cccgaatgag ttcgaggcgc tcgttgcggg
     7801 gccccgaggg gcggtgtttc gcctgaagat caccaacgtg gatgcgtggc gccctggcac
     7861 gcagccacta cccaacggac tcggtgaaat cgaagccgtc gatctggcga acgaatcgga
     7921 gatttggggc cagctgagga atggctgcat cgccggacgc gctctatttc aagatacgtc
     7981 aaccaacaaa cgcgagaaca agcatcgccc tcagaagcga gtcattcctc tcgtaaacat
     8041 taccgccttg caacccgggc tttccacgaa agtttgaaac atgtcgtgct gtacttctag
     8101 tcagagacgc tcgcccggga tgggttaccg agatccgatc acgaacaagg atcgagtcga
     8161 agtcatggga acgtgggact tcaaatggcc attgggcagc aaaatccgcg ttgccttcca
     8221 gcagccgagc tctcgcgaga tcaacgaagc tgatttcctc gaatccgtcc agatcatcaa
     8281 gcagctcgcc gagctctgga tcaaagcggc cccgcaccaa gccgccccga atatcgagtt
     8341 cgattggagc ccgcccccgt tctcccgtgc ggacccaact gatccgcaaa atggcagtac
     8401 ttttgcgttt ccttcgggca agcaggagta tcgcgctcgc gactatgatg tgctgatttc
     8461 gctcgatacg ttaccgaggg gtaagcgcga tcccgccact ttggagcggg ttcaaagaaa
     8521 cttcctgccg tcatcggagt tgggcagctt tgcgcgtcga gtggactacg gagttccgac
     8581 ggcgtatttg gggccgttcg ccggccaagc ctccgcagat ggagagtttc gcctcgcgca
     8641 ctattacaag accaactcca tcgcgcgttt ctacgtcatt catgagttcg ggcatattct
     8701 gggactggcg catacgcacc agagccgttc gcgacgcgcc acgctccccc cggaagccgt
     8761 tgcgcgtttg accaacatcg tgatccattc ccaaaatctg attcgggtcg agtcgagcgc
     8821 ggcgcgctgg ggcgacactg gattggaagc gtcgacaagt atctactcgg gtcaacggcg
     8881 cgaactccgc aaagagatcg cgccgatcaa ggctacgctg cgagccttcc tcgaccggcg
     8941 ccacatcccg attacgctcg aggaggctga gctcgacgat ttcgtggtca gtcagatcgt
     9001 cgagtcgtgg ccggggaacg acgccttctc cgactggccc gcggcggggc cctccatcat
     9061 ggatatccct tatttcgagt gcttgatcgg gctccatcgg gcggcggatg gcttctgcga
     9121 gatctgcgaa gagctgatct cgcgcggcgc gcctttggag ggagacctgc gggccctcgc
     9181 cgccatgtat ccgtgcgtcg agtagcggcg cgctccgctc aggagaagcg tgcgccgtga
     9241 gcgcaatcca gtgcctgggc aaccgcccga tcgaaacgag cggggctcgc cgactcatcc
     9301 tcggcgagcg ccgcggcgat cgcgctgtga agtgcggacg gcaatgcttg ttgggtcgga
     9361 gccagcaagg cggcgagata ttcacggagc agatgatggc cgccgggttg ctgcagttgg
     9421 atatcgataa gtggaaatag agcaatccac atgaatggga agggatgcgg gtgcgcttgc
     9481 cacaccgcta gagctttctc cagcagcggg cgagcgtgaa aggattctcc agacacccag
     9541 cgcgaccagc cctggcacgc gagcgcggcc ccgacgtaag gcaagagctg aacctcttcc
     9601 gctatgacca ccgcccgttc cgcgacttgc gcggcctgcc gtcgatcgcg gagtcgcacg
     9661 tgagtgagca tcgaataggt caagactcgt gatagcagcg tgctatcacc gagcgtgata
     9721 gcgtcccgct ctgcttgcct cagcacgtcg acgccctccc ggcagtcgct ttcggagccg
     9781 ggcagtagca agaacccgag cgtgagtcgg gccagggcaa tctgattggt ggccatggct
     9841 tgcgcgccgt ggctcagcgc gagtcgagcc aggcggagtg actccggatt gagggcatag
     9901 cgcccacggg ccgccagctc gcacgccgcg cccacgcaga aataggcgcg ttgttcggcg
     9961 gaggaatgca tctccacgat tggctcgagt tggcgaatga ggcgctctgt atcgtcaccc
    10021 acctgacgtg agaaatacaa ctgctcgagc cggccgatct cgatctcgat gtattcgcga
    10081 aagtccgccg ccgtgacggg gtttcccagc atttcacgtg cggtgtcgag gttgagacgt
    10141 gcgttctcgt aatcgtggag tgtccagtaa gacgccgcta ttttgcggtg caagcgcgcg
    10201 gtgtgcaggg gggctcccgg tagggaacgc cagagctcga gggactcgct gtaagcctct
    10261 ctagctcggt ggtgctgggc ttgagaaagc caagtatcgg ccaggctctc cagtagctcc
    10321 gcgagcgtcg gtcgataccg cgtttgcctc tgacccacta ggcatctgca ttccgcgacc
    10381 gccagctcat acaactccgc ggctcgtagc ttggcggagg actctgcggc ggccctggcc
    10441 gcgtctcgca ggtggggcag tgcgcgctcg ggttcggcgg cgttggccca atgaatgccg
    10501 agctgcgcga tccactccgg tcgcgcgctg accgcattta gctcctccaa ccgcaaagca
    10561 acacggcgat gtagagaccg gcgcgttacc tcgtgcagtg aacgctccgc ggactcgcga
    10621 atcccgtcat gcgcaaatcg gtagcggccg gccttgaccc actccaagat ctggcgagca
    10681 accaactctt cgagagcggt cgcggtcgag cgggactggg tatcccgcag tgactgcagg
    10741 agctccgcat cgaactcgcg ccccaagatg gcggccattt gtagcgtcgc caccgcactc
    10801 ggagtcagct tggcaactcg cagcgccagc agatcatcga gcgaagaggg gagcccgtgg
    10861 ggagcaagcc caggctcgaa tgcccaatcg ttgtccccgt tcgaccgcag ctgtccgctc
    10921 gcgagcgccg cccgcaagtg ttccgacgca aagaacggat tgccctccga acgctcgaag
    10981 agaaactgcg agagcccctc gggcatggac gtcaaaccga gcatgtcttt ggccatgacg
    11041 tgaatggtgg attggccaag gcgcggcaga ttcacgtgcg agatcgcggc gcgacgcagc
    11101 aacgtgagcg cttcgggttc ttgttccttg cgatacactc caaaaatcag aatgtgactg
    11161 cggcagaact ctcgcccatg ctcgacgaga aactgaatgg acagctcatc catccattgc
    11221 aaatcgtcga ccagaatcag cagcactctt tgattcgagt acgcgagcaa agcgtcgcgg
    11281 aggccgctca ggacgcgtcg ccgcactacg tcggcggggg ctcgcttgcc ggggctcagc
    11341 tcggttcggg aacgtgtgcc tcctacgtac gacgcaatgt cttgcaaagc ctctcgcatg
    11401 cgaggctcgg ccggcgagcg ctgtgaacac tcgtcgttga ggtgctgcag cagcggcgcg
    11461 aacggcgcca gttccgggag caccaggcta ggtccgcgcg tgtcgaagct gtattgggag
    11521 ctcggacaag agaccacgga aaggccgcgt tcgagggcac ggcgggcgac ctcgttgagc
    11581 agccgagtct ttccgatccc gctttcaccc gagactagcg ccagcccgcc actgcctcgc
    11641 tcggcgtcgt tcaccagctg agtcagctga tccaagagct cctctcgccc aacgaagcgt
    11701 gagcggtaca aggtgatggg gtaatctgac ggcgggaagg gtcgaggcgc tgggacgagc
    11761 cgccgcagac tgtcggccac atcccgagcc cgcccaatcc ggtctgatgg ttccttcgcc
    11821 agcaaacgcg cgacgagccg atcgagttca cttggaaccc agggcagttg ttgtgacaaa
    11881 cgcgcgggca cgatgacgcg gtgttgcatc atcaagtcga tggcccgcgc ccctccgaat
    11941 ggccgccgcc ccgcgatcag ctcgtagaga atgcatccga gggagtagag atcgcagcgc
    12001 gcgtccaagg gcccaccgtc cagtcgctcg ggggccagat aggcgagggt tccgtgtacg
    12061 gggccgctcg gctgcgctac ctctctgggc agccctcgct catgcgcctg aaagcaggcg
    12121 ccgaagtcga ccagcaccgg ttgcccacca tcccgaacga tgacattggc cgggctcaag
    12181 tcgccgtgca ctactccttc ggaatggacc agctccagcg cgtccgcgag ctgcgcggcc
    12241 agctcgagca cctcgttcag ctgaacacgc cgtgagccct cttcacccga gctagcgagc
    12301 caagctgcct tctctgcgag ccgcgtcagt ggctcgcctt ggatatgttc catcacgtag
    12361 aagggcgctc cggcgtgctc tccgctgtcg atgaggcgta cgacgccggg gtgctggtat
    12421 cgattcaggc gtttgagcgc cgcgatctct cttcgcaagg gttcacggcg gcgactgctg
    12481 tcctcgttgg gtaacttgag gcaggctctg acgcctgtct cggtatggca gcatcgatag
    12541 acgctgcccg tgccgccttc tccgaggagc gcaccgagct cgtatcgacc gatcacggct
    12601 tgcacagggt tgtccgaacc ttaacagaag aaccccctcg tcgctctgag attcacggcc
    12661 ggtccagagc gcgcatgcac ggacggccgc gcaccgtggc ggctcggttg cgagcgactg
    12721 cgagtgacac ctgggcgtgg ttttactgcc gcggtctaac cgccgacggc gctctcgtgc
    12781 atgcgattgg gggctacgct cgacgaaacg tcggcggaac gttggtgcgc cgagctcgcc
    12841 gcctcgccac gacgcgatct cgcctactgg gcgtgagcgt cccgaggggt cagtcgcgat
    12901 tcactcgcga gggtttgcgc cctacttctg gaactgacgg gcgaactcgg cgagtacgcc
    12961 ttggtttcca cccagtggat cgcccgaacg agagtcgaag ttgtagctcg gcgttcgcca
    13021 atccgggtaa tagtcataca gcagggcgcc gacgaaccct ctctcgtagg cggtggtcaa
    13081 ctttgctcga acgctcgccg cgcgccgctc gaacgcagct gcgctcgggt cttccagctt
    13141 gacgccaatc gcacccagca agatgggttt tccgagcgcg gcggcaatgc tcgagttggt
    13201 ggcaacttgc tcggtaatcg actcgttcgg cgcatcgaaa tcgtgcacat ctaccagatc
    13261 gacggtggga tgggcatgga gctcggcgta gctcgagttc acgccttgat tgctggtgcc
    13321 cgtggagtaa ccgttgttta cgccaatggc gatcaggtgg ttgggatcag cgacacgaat
    13381 cagagtggac atccgctcga cgaagtcatc catggccgcg aaatcgtccg agccaccttc
    13441 atgcagcagt tcccacgcca aaatagcggg ttcgtcacga aaatgctgta cgagctggtt
    13501 gacgtaatct ggataggaca gcgtgtaacc accgtagctc atggcgtaac cggacctgaa
    13561 ccagtcgtcg gttcgcgcgc ctcgggtgca gtccgagtat tggttctcga gcacgaatat
    13621 caggcgaact ccggcgcgtt ttgcatgaga cacgaggcgc tcgaacgccg agtagtcggc
    13681 gccggtgacc cccgcatagc tttgaaacgc ccacacgcgc aggacacgcg cgttcagctc
    13741 caccagatca tcgaacactc gagtcacggc cgattcggcg tctggaaact cggatattct
    13801 gcagcccccg gccgcccacg cggcccccca gctgttcgct cccacgaagc gataaggttg
    13861 gctggcgagc gagagcgtag tccccgttcg cctcacgaac ggatccccgc tgatgcacga
    13921 actcgcaggg cagctcgcat cactcgagcg acacccaatc gggttgggtt gtacgctgca
    13981 gccgtgttgc gcgtcggatc ggcattgttc ttcagtcgtc caagccgaac agggatcgtt
    14041 cgtgtcgatc ggagcacctc cgatcgggtc cgcgaattgc tgactgcagc ccagggcagc
    14101 gaacagcgcg gcgagcgcga gtgatgcggt gaagttcacg gcgaccactc cagccccact
    14161 ccgaggttca ggctggggcg tccgaaatcg gtgatttctt tgctcgcgac gcgcacggtc
    14221 gttttgggga gcgagagccc gagggtcgcc cacgccaaca ctcgtaggtt cgtggtcagc
    14281 tcgagggcgg tcgtgccacg caaccagggt ccccatgtcc acagcgccgc ttcggcgttc
    14341 gtgtaaggcg cgcgggcggc accgctaaac gaggtgcgct cgagctgtgc gcccgcgctg
    14401 cccccgattg cgagtcgagc ctcgcgtgaa ggctcgagcc acactcccag cttataagaa
    14461 ctgctgcgcg cttcgacctc tccctccgtg cgttgaagcg tcgagccaga caggggcagc
    14521 gaagcctcgg ccgccaatgc ccaatgatcg cccgtgcgcc aagccacgag gaaccagccg
    14581 ccaaaaccag gactgaaggg cggcgcggtc agcacgctcc cgcccgccgc aaacgcggga
    14641 cctagccgct cgtgcttggc ggctgaaaca ggccgagccg gagccggttg aagctcttgg
    14701 cgctgccgga gtggctccga gtgggtgctt cgttgacgga cttgctcgta ctcgagccga
    14761 gccgcgcgca acaactcgac ggttcgggtg gtcacgagct cagccgctgc gggatcttgt
    14821 tgcgcctcga tcggcaggct atggcggatc agcttagagc tcttcggcgc cttcaccacc
    14881 aggttgatct gacccgatga cgtcgggaga atctcgattg cggcgatggt gttgggctcg
    14941 cgaagctcat ccgagagcga ggtcgttctt gccttccgca cgtccacctc gaagccgagc
    15001 gcctcgagct cttgccgcag tcgtcgtgcc aacggcgagt cttcggtatc gaggatgagg
    15061 accgccaaag tctccgaatc cgtacttggc ttggcttccg cgtcttgggg cccggcgcag
    15121 ctcacggccg gctcgcacac cactcccatc gacaacgcca ggagtgccac gaaacggtgg
    15181 gcccgccgtg cccgaaccgc gcgagaactc tccctggcgc accggtcagg gcaacaacga
    15241 ccgtgcaagc tcggcatgcg ctccgtccgg gtatcgcgcg aggtagtcgc gcgccgcctt
    15301 accagctgcg acgcgatccc cggtggcacg caaagcttcc atgtaccgcc ccgaggcttc
    15361 tcgcgccaag ctcccgcgcg gctgctctcg caagtacgtc gcgaaccaac tcgcagcttc
    15421 ggcataggtg tggcgctgat cgaacgctac cttgccgagc gcgaaggcgg cggcggcgct
    15481 tctcgtgtca cccggaaacc ttctccgcag cgtgagcaac gcttcgctcg cacgatccgg
    15541 gcgacccgaa acccgcgcgg cgtcccccag cccgagtaac tcaccagcgc tggcggtctc
    15601 gcagaccttt tggaagccgg tttgctccgc gttcgcaaaa gcggcccgca actcgccgct
    15661 ccccaacaat gcctgccaat ccggtacggc gttcttgggc gcgatcgaag gcttcgctag
    15721 atgctgcgag agttccgagc ggggccgtgc agcttgctgc gacaacggtt gcgcttggat
    15781 aggggcttcg ggcactgggg ctggtggcgc gggtgggatt ggcacggcag agagggttgg
    15841 cttcgcgctg gaaccagtga gttccaagcg gtcctctcgc acggagacga acagcttctc
    15901 gcccgcgcgc acggcgcggc cagctcccgc gaccgagcca gagactcgca cgctgccttc
    15961 gctgacgaga atcgtgaact cttcgaggct cggattccat ttcatctcga agcgcgttcc
    16021 cgtaaccacg accatcagcg gtccggccac cagctgccaa gtgctgtgct cggtgtgcac
    16081 cacctccgcg cgtagaatcc cgctttcgag tgcaatgcgc gctccgtgcg gagtgacctc
    16141 gactacacgc gcttgcgacg catgctcgag ttgcagggcc gtgccgtccg agaaggtcag
    16201 aacaaccggc ttgaccttgg ccccgagcca agtgtcgaca cggccatgtt ggccattcac
    16261 cgcgaacgat agggctcgct cgtcccgagt ccacaccatc acggctaatc cggaacagag
    16321 caccaaggcg gccgctgacg cccaaccgag ccagcgccga gtctcgactc gccgagtatc
    16381 agctcgtcga gctatgcctg gatcgctcgc gaggccgaca tgcttctcca gaaatgcgcg
    16441 gcgcgctcgt tcgatcgcct tttggcggtg agcactgtgt tgctcgagtg catgcgccac
    16501 gtcgcgcccg atgcgctcga cgggtcctac gggcttggcc atcgtccacc tcgctcgagc
    16561 cactcactca agattcgatt tttgctcgct gcggccacga aacgggcttg ggcgcgtgca
    16621 atccgccgtt tcgctgtcga aagagatact ccgagcacct cggccatctc ggtcagctcc
    16681 atggagtcga tgaagcgaag gctgaaagcg acgcgctggt cggtaggaag ctggtccaag
    16741 atcccatata ccgcgtgcac cgcgtgcagg gtctgcggat ccagccctgt cgtgactggc
    16801 tcatatcgcg cctcttcggc gtcatcgacg aagtagcgaa gccacgcgcg ccgagcccga
    16861 gccctcaaga gcttctgggc cgtgcgtgat gccactccga acagccatgg cttcagcgca
    16921 cttggattct tcaagctcgg cagcgacgcg aatgctcgcg tataggtctc ctggagtaca
    16981 tccgccagct cgcggtcggg gcccaaaatg tgagtcagca tgcgctccac atcacggacg
    17041 taacggttga agaattcagc ttttgctgcc gggcgccctt cacggatcgc ggccaccaag
    17101 gcgatgtcgt cccctgcgaa gcccacgggc agcgtcacga cgggagacgc ggagggccgt
    17161 gaatagttgg cgcgttgtga gctgggcatg acgaggcgcg ctaccgaggt acgaagcccg
    17221 tcgtgtaaca tgagtccccg gacgcggaca gcgaggctca ttcttttcga ccgctccaat
    17281 gaccgcgctc ggcaggagtg ggcccagagg cgctgcgacg gaatggtctg cccgagacgc
    17341 tcttgcaact ctgtttcgag ggcttgggcc acgccgctgt cagcccgcat tcacggcggt
    17401 gctcgcgatg tctcgcaacg ggaaattccg tcggtaattc ggttgctgac gcgtgctaat
    17461 gaacccacag acatgaagcg atcctcattg gttttggcgg ctgtcgcggt gatggccggc
    17521 gtggtgtggc ttgcctggcg cgaggttcgg cccacacctc gagaagccgc gccgctggcg
    17581 gcagaggagg aagcgggcac cccagcgccg ccgccgctca gcgcttcggc ggcgcccact
    17641 agcccttcca cctttcgcga agcgacaacg cccatttcag cttccgcagg gcctcgcgat
    17701 gccctgccca gccagccatt gccggttcag ccgaccgacg ccgaactgat gaattcgctc
    17761 catgaattgg gctggtcgaa tccaaccaaa accttggaat tggctcgaca aggaaactcc
    17821 cgctttccat cgagtgccaa cgcacccgag cgcgcatcgc tcatcatccg ggccctatcc
    17881 agcctgggtc gccaggaaga ggcccgggta gaagccaaga agatgctgga gacgtatccg
    17941 cgctcgagtt ggaccgaaga tgtgcacaaa cacatgttcg tgaatccacc gacgcacccg
    18001 gccgagcatg gctacggcaa ggagttcgaa ctcgaatgac ggagtgcgat acggcggtca
    18061 cttatcgtgc cgaccaccgc acttggctcg tgtccagatt ctgcggtgcg ctacttcaga
    18121 ttcgtcggca gttcgtcacg ggtgatcgta tgactgtcgg cgtaggcaga cttgatggtt
    18181 tcgggtgtgt tccaggtgta accctccgca gcggtattga gatagttaca ccagacattc
    18241 catagcagcc acgttgcgcc cttggcccac atatcggccg gttgcggcaa agtccccgtt
    18301 tcgtgcagcg ggaggggcat cgtgttgccg aacacgctct tcacgctgtt ccattgagtc
    18361 agaaacggct gggtgccctt gtcatattgg tcgatcccgc cgagatcgac gtactctttg
    18421 cctgggtaat aggcggccaa agccccgtca tgtccgaatc ccaatgtcca aatgacgttg
    18481 tgtacgtcct tcacattgtt gatgtaatcg acggtgtact tccacagttt ctgaaactgc
    18541 ggccccgtcc ccttcgacca ccagaaccaa gcgaactgat cgacctcgtg atacggcacc
    18601 aagatgattg gcatgcccat gtctttcaac tgggttgcca tgcgatcgag cttcgaattg
    18661 agcgacgtgt tctcggcggt gcctgcgacc gtgaggttgt cgtagttcgt caccgtgagc
    18721 ttggagttct cgtaagtgtc cgcgtttgga ggcgccccca tgtgataacg catcatgatg
    18781 atgccattcg ccgccgcgta gggttttgcg tacgttgccg agccctcgta caggaagtca
    18841 ccaccccaaa ccgccggata cttcccagtc tgctggttgg tatacgtgaa aagtgcattg
    18901 cgatcgggat cccagctctg ctcctgctgc cccgagagca cactcttggt atagatgctg
    18961 tacagataac agagcaggtt cttcgttgcc tgcgtcgcct tcggatcgat cggcgtaacg
    19021 gtgcagttcg aggttccgcc accaccaccg gtcccaccga cgttcgtcgt gcccccgctc
    19081 gtgccacccg cgccggagcc accgttcgcg gtgccacccg tgctcgagcc tcctgcgcca
    19141 ccgggtgcgg tgccgccgct gtgtgttccg ccctgtgatc ctccaccctg cgcagtactg
    19201 cctccgttgg gtgttccacc actcgccgtc gccccgcccg tggccagagt gtctgtttgt
    19261 ccaggggaac cagtgctccc gccgttggac agaacaccgc ctgcggacgc cccgccggtg
    19321 gacacactgc ctccgctcgt cacggctccc ccgctggaag tcttgccgcc tgcgggttca
    19381 ccgagagacc caccggactc ggagctcggg acaccttcgc tcccggagca tccgctcaaa
    19441 gccaatgccc caatcagcaa cccgaggcgt gctcggcgca gaaccagctg ggtaggaaaa
    19501 ttccgttctg cggcacaatc tgaaacgagc ggtggtgttt ggttcgtgga ctgcatgaac
    19561 ttctccgggt gcgtgctcgg caagaactcc gcacggcgag caaccgcggc gtcgatgcgc
    19621 cgcg
//
LOCUS       DASTKU010000465         3289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_980811, whole genome shotgun sequence.
ACCESSION   DASTKU010000465 DASTKU010000000
VERSION     DASTKU010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980811"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1820
                     /locus_tag="VFQ61_16075"
     CDS             138..1820
                     /locus_tag="VFQ61_16075"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET9956025.1"
                     /translation="MQSFTQVSRGLAEIAGLLEFAGEPKFKVKAYANAAEVVDTLGDD
                     LASLVEQNRLQELPGIGRALEQQIQELWNAGTSALLTRLRSEQPEGASELLQVEGMTP
                     RRIRALHDALGIRSVPELRAACAAQRVRDVAGFGKKTEERLLAACERWLTRGDVEQPS
                     TLLSVALELASFLERELRKTLDEVELAGALRRGEETVREIEFVVRGDLEAALQQLCGL
                     RQVIRVDFENHLAYLGGGLRLRLHASGPGWGTALVLATGTAAHVTALRARAERRGLEP
                     LLPATETEQGLYESLRLSWIPPELRQGTNELELASSGDFADLIAIDDIQGMVHCHTTY
                     SDGKAGIEEMALAAQALGMKYITITDHSPSAHYAGGVTLDRLRAQWDEIAAIQERVTI
                     RILRGTESDILSSGELDYPDEILEQFDVIIASIHARHRMDRAAMTDRVKKALSWPGFK
                     IWGHALGRILNHRDPIDCDVPVILDALAQSRGAIEINADPHRLDLPPAWIPAARERGI
                     PFVVSVDAHSTRGLGVVNYGVTMARRGGLRKREILNALPAEDFASRVRPATG"
     gene            1893..3197
                     /locus_tag="VFQ61_16080"
     CDS             1893..3197
                     /locus_tag="VFQ61_16080"
                     /inference="COORDINATES: protein motif:HMM:NF016883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytochelatin synthase family protein"
                     /protein_id="HET9956026.1"
                     /translation="MPSSSRVSSESAVALEPKSFYRRPLPEGHVPFSSEEGRQLFRES
                     LAGGGMECWFALSEQYHTQAEPAFCGLGSLVVVLNALEIDPGRVWKGPWRWFSETLLD
                     CCMPLNVVQEQGVTIDELACLARCNGARAQTFRAELADVATLRTAVELATGRPRGPFV
                     VASYSRAALGQTGNGHFSPLAGYHRERDLVLILDVARFKYPPHWVPLEQLWYAMRPVD
                     PATKRSRGWLVFDRGELRPAPVYLRLLAPHGIGDLVRLLLERSPALLAAAPDTAMDAW
                     ISGWVANLASERLEKFWEILQAAPETHTLSAEHYATIERLLTELRGSRAFLLIQTALE
                     KARALRAAPDAALLEHPPLGVGSLRTSLIGAELLTLLLLAMPDSVMRGLPARQRGFAH
                     ELEADMQGRTLLVQETQALREQLNSLGALRNASHESSSCCSA"
BASE COUNT          581 a          991 c         1125 g          592 t
ORIGIN      
        1 acccttggag gtgctgctcc agggcatggc caagcgggcc cgcggtatga agaccgacaa
       61 gatcaagaac gaggatctcg cgcgcaccgc tggcccacct ccggaagagg actgagaaga
      121 acgagaggat cgagaacgtg cagagcttca cgcaggtatc acgcgggctc gccgagatcg
      181 ccggcttgct cgagttcgcc ggagagccga agttcaaggt gaaggcttac gccaacgcgg
      241 cggaggtggt cgacaccttg ggggacgact tagcctcgct cgtcgagcag aatcggctgc
      301 aggagctgcc cggaattggg cgagctctcg agcagcagat ccaggagctg tggaacgccg
      361 gaacatccgc gctgctgacg cgcttacgca gtgaacagcc ggagggcgcg agcgagttgc
      421 tgcaggtcga gggaatgacg ccgcgtcgca ttcgcgcctt gcacgacgcg ctcggaatcc
      481 gatcggttcc cgagctgcgc gccgcatgcg cggcccaacg ggtgcgcgac gtggcgggct
      541 tcggaaagaa gaccgaggag cgcctgcttg ccgcctgtga gcgctggctg acgcgcgggg
      601 acgtcgagca gccgtccacg ttgctttccg tcgcgctcga actcgcaagc ttcctcgagc
      661 gcgagctgcg gaagacattg gacgaagtcg agctcgcggg cgcccttcgc cgcggcgaag
      721 agaccgtgcg cgagatcgag ttcgtcgtgc gtggggatct cgaggccgcg ctgcagcagc
      781 tgtgcgggct acgccaagtc attcgcgtcg atttcgaaaa ccaccttgca tacctcgggg
      841 gtgggctgcg tttacggctt cacgcttcag ggcccggctg gggaaccgct ctcgtcctag
      901 ccaccggaac cgccgcgcat gtaacggctt tgcgagcgcg ggcggagcgg cgcggtctcg
      961 aaccattgct gccggcgacg gagacggagc aggggttata cgagtcgctc cggctgtctt
     1021 ggattccgcc cgagctccgc caaggcacga acgagctcga gttggcctcg agcggtgatt
     1081 tcgcggatct gattgccatt gatgatatcc aaggcatggt gcactgccat acgacctact
     1141 cggacggcaa agcggggatc gaggagatgg ccttggccgc tcaggctctg gggatgaagt
     1201 acatcacgat tacggatcat tctccgagcg cgcattacgc gggcggcgtg actctggatc
     1261 ggttgcgggc gcagtgggac gagatcgcgg ccattcagga gcgggtgaca attcgaattt
     1321 tgcgagggac cgagtcagac atcctcagct ccggagagct cgactacccc gacgaaatcc
     1381 tggaacagtt cgacgtgatc atcgcgagca tccacgcccg gcaccggatg gaccgcgcgg
     1441 cgatgaccga tcgcgtaaaa aaggccctgt cctggccggg gttcaagatt tggggtcacg
     1501 ccttgggtcg catcctcaat catcgcgatc cgatcgactg tgacgtgccc gtcatactgg
     1561 acgcgctcgc gcagtcgcgt ggcgccatcg aaatcaatgc tgatccccat cgcctggacc
     1621 tgccgccggc ttggattccc gcggcccgag agcgggggat acccttcgta gtgtccgtcg
     1681 atgcacactc gacgcggggc ctcggagtag tgaactacgg tgtcaccatg gctcgacgcg
     1741 gtggtctccg taagagggag atcctcaacg cgctccccgc cgaggacttc gcgtcgcggg
     1801 tgcggccggc gacaggttga gggggcgaat ggcctcgtgc ttcagacgcg aagtggaaat
     1861 gactcgagcg tgacgtatgc tcctcgcgca acatgccgag ctctagtcga gtgagttccg
     1921 agtccgctgt agctctcgag ccgaagagct tctatcgccg accgttgccc gaggggcacg
     1981 tcccattctc gtccgaagaa ggccgccagc tgtttcgtga gtcgttggcg ggcggaggga
     2041 tggagtgttg gtttgcgctc tccgagcagt atcacactca agccgagccc gccttttgcg
     2101 ggttggggag cctcgtagtg gtgctcaacg cactcgagat cgatccgggg agagtatgga
     2161 agggaccttg gcgttggttc agcgagacct tgttggactg ctgtatgccg ttgaacgtgg
     2221 tgcaagagca gggggtcacg atcgacgaac tcgcctgtct ggctcgctgc aacggcgcgc
     2281 gcgcgcagac atttcgggct gagctggctg acgtcgcgac gcttcgtaca gcggtcgagc
     2341 tagcaacggg gcggccacga gggccgttcg tcgtggcgag ctactcgcgc gcggcgctcg
     2401 gtcaaacggg caacgggcac ttttcaccgc tcgcggggta ccatcgggag cgggatttgg
     2461 ttctgattct ggacgtagcg cgcttcaagt acccgccgca ctgggtcccg ctcgagcagc
     2521 tgtggtacgc gatgcggccc gtggatcccg cgacaaagcg ctcgcgagga tggttggtat
     2581 tcgatcgagg tgagctccga ccggcccctg tttacttgcg gttgctcgct cctcacggaa
     2641 tcggcgacct ggtccggctg ctgctcgaac ggagcccggc gctgctcgcc gccgcgccgg
     2701 ataccgcgat ggacgcctgg atcagcggct gggtcgcgaa cctggcctcc gagagactcg
     2761 agaaattctg ggagatctta caggccgcgc cggagactca cacgctcagc gccgagcact
     2821 acgcgacgat cgagcgtttg ctgacggagt tgcggggcag tcgtgctttc cttctgattc
     2881 agaccgccct tgaaaaagcc cgcgccctcc gagccgcacc ggacgcagct ttgttggagc
     2941 accctccgct gggcgtgggc tcgctgcgta cgtctctgat tggggcggag ctcttgaccc
     3001 tgcttttgct cgcaatgccg gactcggtaa tgagaggttt gcccgctcgg cagcgcggct
     3061 ttgcccacga gctcgaggcg gacatgcagg gccgaactct gctcgtgcaa gagacgcagg
     3121 cgctgcgcga acagctgaat tcgcttggag ccttgcgaaa cgcgagccat gaatcgtcgt
     3181 cgtgttgctc ggcgtgatcc gtttctacgg gcgcagcaca cgaggcaacg agcaggcggc
     3241 cacgagcacg agcccgacga tcggcaggct gtgccccacc aagaggtga
//
LOCUS       DASTKU010000466        21136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_535789, whole genome shotgun sequence.
ACCESSION   DASTKU010000466 DASTKU010000000
VERSION     DASTKU010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 21136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21136
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535789"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFQ61_16085"
     CDS             <1..340
                     /locus_tag="VFQ61_16085"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HET9956027.1"
                     /translation="FFLVESAGASFSPLSSQETNFDLALRLEPAIWILVAPDSLGVLH
                     DLTTTLALMRERGRLPDYVVLNQARPPDASTGTNAEELSRLRIAQPELVVRRDSADLA
                     AFAERLSRHA"
     gene            complement(348..3977)
                     /gene="mfd"
                     /locus_tag="VFQ61_16090"
     CDS             complement(348..3977)
                     /gene="mfd"
                     /locus_tag="VFQ61_16090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET9956028.1"
                     /translation="MSAADSLANPALSLALPEDRRILTLRELSAQVLQRRGILHVRDA
                     SGAAPALCAQALCDSGAKHVVYVAPDLDRARRASADLSALSRGLPASSRVAPGSTRAA
                     QSVGPLMFAATETSPYAEVHGDRRAAMARAATLFHIAAGLPFQFVVITASALGRRVVR
                     PEVLRQAAIRIEAESELDLAQLASRLTFAGYLRTPVVEDPGTFAVRGGLLDVWPAGAE
                     GPLRVELYGDLILTIRKFDPDDQRTIGELTEAWLPPAREAIIDAAAESRARNVLRDLC
                     DRVDYPSTKARAFIEDLATGHAYFGLEGYLPAFHETVPLLDYFGEKAVVVIDDPPAVV
                     RALREDAAQGREGEGQRVQTPHFGFEQLYTDESQIARALEQRACLAAHASLVAGSTAT
                     TGLDALEGGSADAPSVWIRDHGDLTREGKLARTTRGKAASLDPVITRITLWQDAGLSV
                     IITARARTQAERVAALLSHRGVKVALWDPDTANDSQLSSVLVAVAPMARGAICPAERF
                     VIVTEEEIFGQRTHRAPAKPRAARAMLEDLRALEPGDYVVHVDHGIGRYVGLERRQVG
                     DVSVDLIVVEYGGGDKLFLPVYRLNQIEKFSGGDAQPKLDRLGGQTFSKTKAKVQQRV
                     RQMADELLRLYAERGALQKDALPAPDDDYAAFEASFPYDETRDQTAAIQDVLKDLASE
                     RVMDRVVCGDVGFGKTEVALRAAFLCANSGRQVALLCPTTVLAQQHYMTFSQRLQDYP
                     IRVKAMSRFQSRADQVETLKGLKDGSVDIVVGTHRLLSKDVHFKHLGLLVVDEEQRFG
                     VTHKERIKQLRTSVDVLTLSATPIPRTLQLAVGGLRDMSIISTPPVDRRAIRTITSQF
                     DPELVRQAVERELERGGQVFYVYNRVEGIYERAERLQALVPRARIAVGHGKLTEDVLE
                     RTMLDFVAGEYDILVATAIIESGLDIPRANTIIVDRADLFGLAQLYQLRGRVGRSSER
                     AYCYLLVPPPSQLTDESRARLEALERHSELGSGIHIAALDMELRGAGDVLGAEQSGFA
                     ASVGFDLFCHMLEEATHELRGEPVVHEVDPELSLDVDALIPDNYVSDVGVRLSFYKRL
                     ASASDEAEVADIASELQDRFGEPPVEARRFVELMRLKTELRKLRVLGCEASERSVSLH
                     LRDDTTLDPVKVGELVAQKKSPYRLTPDGRLTRRPLENETFADGLALADRMLTELARC
                     LKTGS"
     gene            complement(3992..4963)
                     /locus_tag="VFQ61_16095"
     CDS             complement(3992..4963)
                     /locus_tag="VFQ61_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9956029.1"
                     /translation="MNQGRIRHGRMSQGLTHSPDTHLGLRRHLAGWPLAALAVAFPLL
                     AVALALPRAVHSEHVPLPLPDRAVIIREQAADRERAATVGTKALPFSVRLVGEWMRRY
                     GSATFSDNPTRAAEARAELVSSVRNVLRSDPQTGQAELLRLRALQSDLFLSAVARWRG
                     SHGRPRLDAQELDSDLRELGGEFVASCTSNGWLDENGRLRLSADQLFILYRVHWTELT
                     GLLQRTPFRPGLEEFRLYYRLLLENADDEAHAPAARRLEYAQALAKLDPDYPGEVARG
                     VLLLELNRPEAAFEAFQSFLKRRPDGPWVVRARNYALAALARIPEEE"
     gene            5049..5351
                     /locus_tag="VFQ61_16100"
     CDS             5049..5351
                     /locus_tag="VFQ61_16100"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9956030.1"
                     /translation="MQEKRGHPRVPIQLRATCHRASGGSFVAQVVNVSVGGMFLNSAE
                     VLQFGEELTIELELPRARAPLRLPAIVRWTLAEGFGVQFGLLGARDTHALVELTRG"
     gene            5502..6107
                     /locus_tag="VFQ61_16105"
     CDS             5502..6107
                     /locus_tag="VFQ61_16105"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET9956031.1"
                     /translation="MSTIPRSPQDFFQIWLPSAFGPVAAKPSGSGQTSAPPNSPGALV
                     FWIGDEAPFALRLSAGKLRVERALAPDCVVQVILSEADFEPVIVRGAEALMAQGNPEK
                     QLSVLHALSLDKERVELIRGVPGSVAFVLPAGAVEHRIVLTPGVRPKALGPTECTVRC
                     ALNDFLGMQRGEANPFDLMMNGRLQISGDAQIPMALSSLLM"
     gene            6104..7090
                     /locus_tag="VFQ61_16110"
     CDS             6104..7090
                     /locus_tag="VFQ61_16110"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9956032.1"
                     /translation="MNAGDVNASTPQAASRAGRGVPPRLCALSVDLDEITHYHAIHGL
                     RAPEPACANLVYDLAIARCSEFARDFEVPVTWFVVAADLARSANAKRVRELASAGHEI
                     GNHSLTHPYELVRLTPSEQWRQIDGALELFQHELGYRPRGFRAPGYSVNDALLTMVAK
                     AGHLYDSSVFPSAPYYAAKALALLALRLRGRHSKAILDSPELLTAPRRPYRIGTPYTT
                     RGAGLLELPVQVTRGPRLPFIGTALVLAGELGARLLTELVIGEPFINLELHGIDWLGD
                     GDPLAPLRGFQPDVRIEHQRKRAIFGGVIARMRAAGYRFVTLSDAARQFAHL"
     gene            7305..7667
                     /locus_tag="VFQ61_16115"
     CDS             7305..7667
                     /locus_tag="VFQ61_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956033.1"
                     /translation="MMTRVWFSLVSSVGLLALNVAGCASEAPDEPQDPQSVCQRFCAN
                     TRQLSCPNVNLCVSQCLDGRKAVSPNCLPLTDATMRCYAARPATDFYCDPILSKPVPL
                     PGVCADEVAAQTSCATGR"
     gene            complement(7766..8992)
                     /locus_tag="VFQ61_16120"
     CDS             complement(7766..8992)
                     /locus_tag="VFQ61_16120"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolB protein"
                     /protein_id="HET9956034.1"
                     /translation="MGELVVTGTQQERLPRIAILPSLSPDLEDVIVRGVVRRDFELTG
                     LFDLIPDAKAPVGLYGFDDAIDVPAWRNLGAEAIVKVAARADATPGKIQVLGIAYFLN
                     VGKEPVYQKKIVVSKADARVTAHRITDALLGALTGRPGGFASELTFSARWARSRRIFR
                     VDADGNGLTPLTNANDHAIAPTWGPGHSLYYLLSRDYAPYKLFTLSGTTEKPVPIPFT
                     TSLYGVSFDKAGARMALAVSSDNGESTIFVGSSDGSQMQRVSKSQLATHPVFSPSGKL
                     AWIGGDAKQGSQRVFLDGKPVSPAGFTASGPTFCDTEDGIRLVYAVAVGGDRQDLVIS
                     AEDGRGIARLTQDQGSNYSPACSPDGRMLAFFSTRNKQPGVYLMSMKRWKTQQISSQT
                     GEALRWAALPPVPNAP"
     gene            complement(9067..10014)
                     /locus_tag="VFQ61_16125"
     CDS             complement(9067..10014)
                     /locus_tag="VFQ61_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9956035.1"
                     /translation="MSSSASPLDRTFEPHEVALAIAFSAAVLSGFFTLLVLAGQSKSH
                     VPAAPPPAPSTIPIAVRPVLDELPLLKLGGKKVRPKLPEMWKKNPPVQRYEATSAPST
                     KASKTPDAIPSTPLPKPDASAPPPDAEVAKQVDQVLLDAGPDAAPTVEGEGSPDGVKE
                     GTETDPLKARAVSAYRAKILAWFNARFRPPTGEIPCAELKKLTSRVVVAVGQDRRVTS
                     YEVVSPSGNSIFDERTRSTMDRIVGEELPPPPPLYPDILGTTQPVSFSGRSAQCNDTA
                     PNPSPQPPSSPESPAAPAPAPPPPSEPAPPSEPPSPAEP"
     gene            complement(10022..10492)
                     /locus_tag="VFQ61_16130"
     CDS             complement(10022..10492)
                     /locus_tag="VFQ61_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9956036.1"
                     /translation="MGMQVGSRRRGGFRDINVTPLVDVMLVLLVVFMVTAPLLTTGLR
                     IDLPEVKAAQTPIKDARLLVSITKEERVLFGEEDVTGRVDAALRENQRVQKERELYIR
                     ADKSARYGIVAEVVAAARNSGVEALNLLVEPDVEEVPDAAASGVAPSAPPNRKR"
     gene            complement(10496..11194)
                     /locus_tag="VFQ61_16135"
     CDS             complement(10496..11194)
                     /locus_tag="VFQ61_16135"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9956037.1"
                     /translation="MTAQPEAAPPKAIDPLALMLGSSGVVLVVLVLLIVAAALVWLIW
                     FLKFAQIARLRAALRSFERAAEGVSRASDLLSLAERYKTSPGGRIVIALARRREAAAG
                     GAELLGAVARRALTSEQQRATVLMPTLSSIASAAPFVGLFGTVWGIMDAFLRIGVEKS
                     ASLPVVAPAIGEALIATAFGLVAAIPATIGYNYVDKRISDLMDELAASSDVWVETLAE
                     EERASPYGRARNSR"
     gene            complement(11207..11743)
                     /locus_tag="VFQ61_16140"
     CDS             complement(11207..11743)
                     /locus_tag="VFQ61_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9956038.1"
                     /translation="MRTVALALFVSCAALAACGSDPPPPARSPVPPPPPAPPAAEQPA
                     NPKPSDNPTQSNLVISDEIKKACGISDAEAYFAFDSANIRAQDRAVLKKLADCFTTGP
                     LKGRRMRLVGHADPRGDDEYNRVLGQRRADNVKGAITTAGLESSRMETTSRGEDDAVG
                     TEEASWAKDRRVDVLLGS"
     gene            complement(12017..12355)
                     /locus_tag="VFQ61_16145"
     CDS             complement(12017..12355)
                     /locus_tag="VFQ61_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956039.1"
                     /translation="MNPADPVLEAAWKRVLDHWDEEQAHTGFLDYCRLSGRLVEAAVR
                     YRGMTGDRARAELAEKKLKAVALLALTQLETTRTEPRPVNPRRSGYVLISFVFLLGII
                     GLLAYLRATR"
     gene            complement(12383..12811)
                     /locus_tag="VFQ61_16150"
     CDS             complement(12383..12811)
                     /locus_tag="VFQ61_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956040.1"
                     /translation="MAEPLDARTFDARYRDLLSQVGRERGNTGCVECERCQGCSACTF
                     CSDSERLLRCHFCVRCQGCTDCEHCRGSRGLIGCQHCVECENCSSSSYLVRSVGLSAC
                     TYCFGCVGLANKDFHILNEPYSRAEYFALTRRLLRELRIG"
     gene            12965..13633
                     /locus_tag="VFQ61_16155"
     CDS             12965..13633
                     /locus_tag="VFQ61_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956041.1"
                     /translation="MFGRLIFVVGVASACQPVAPAAGPGNANQSAASERAPARPARSA
                     VGSADPEWESGWSKRWLARQALTLELPDAAHWRKGTKVRSFVIEHPASHSVLKVESER
                     AERLVRPADCEQRARLAWPEAPRGDDASRVDARRLDVPPGYVTQVEVLVLPLQEDTAE
                     GHVLAFGAAVGRCLALHFTTRASGPERFERIAERLGLVTGRLLPSITPLSADDRVQTE
                     PLDR"
     gene            complement(13680..14930)
                     /locus_tag="VFQ61_16160"
     CDS             complement(13680..14930)
                     /locus_tag="VFQ61_16160"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HET9956042.1"
                     /translation="MLPPLRSWEKLASLRLLRLALCAVLFWSAQLVGNLSSARAETPA
                     LSTERTGTTGSVDSSDAPASPQDAPRLAPGRLNATRLPDGFNTYDGGWIRFHYHPQTR
                     ERLQALISEAVPFRSELAERLGVPVLTQVRVDLARTPGEMATLAPPEAPYPDYAAGVA
                     YPQIGLTLLSVAPLHPNSQHDLVQVFRHELAHVGLHEAVNGAHVPRWFDEGFAVFVSG
                     ETSLNRLQTLWMATLSDKLIPLEKLDEGFPAHESDAEIAYAQAVDVVRFLVRKREQFR
                     FQGLVERLRAGQDFQAALRDAYGADMFTLEQEWRDDAAKRYTFWPVLLSGSVVWMGVL
                     GLFLWAWRKRRSRSQATLARWAAEEAREDAVRMRQAASSPPPRVHIVLTQSAPPGAPV
                     GMPPPNLEAEVPRIQHEGQWHTLH"
     gene            complement(15013..15750)
                     /locus_tag="VFQ61_16165"
     CDS             complement(15013..15750)
                     /locus_tag="VFQ61_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956043.1"
                     /translation="MRFDRVLSAAWLGALASAACGALRGEPLVRLSEIAAGGALIFGS
                     LLGAWNLAQPLSRRAHAVLCGIALAAVPLSLFGQILKKATHHRPLGAVTFAVGAVVVS
                     LAAAVVAARWLSFCSRRSPKVRNLLTRALDVAAASAVLLLWFKLLGAPPTRSGALDAA
                     IGLGAAGLALRWGALPLALSRIGGVAWLLTLVVGVVSTLGAQPAPSEAHDLLASAWRW
                     LLEAPAPRTALPVWLERVHFSRVARLV"
     gene            complement(15747..16802)
                     /locus_tag="VFQ61_16170"
     CDS             complement(15747..16802)
                     /locus_tag="VFQ61_16170"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9956044.1"
                     /translation="MKKKWTLVTGAAGFLGATVVRQLVENGVPVKAFVRPGANLKVLE
                     NLPRDLCTLAYGDVTIEASVYRALIGCDRLFHVASTYKYGRQHQRDVLRSAELGTQAV
                     LAAAKQHELPRIVVTSSTAVLGVSQGDQLLDESSENQLQDPEPYVRAKIAADRVVQEH
                     VKAGMPIISVLPGAIFGPGDWKPTHNGQGLLHYVKTPPNRKTPTTDGGNSFVDVEDVA
                     RGHLLAMAKGRVGERYILGGENLTYRDFFTLLHELTGLSEPGPTPSPGLLQLVARFME
                     FSARWAGRDPLLTYRLARDYAYSRVWVSSAKAERELGYSFRPARETLARSLRFYLTQG
                     FIEQPLANRLRLELRPV"
     gene            complement(16952..19453)
                     /locus_tag="VFQ61_16175"
     CDS             complement(16952..19453)
                     /locus_tag="VFQ61_16175"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9956045.1"
                     /translation="MRFSNRLAARILAALGVSFFGQVALAAPAFAPPGRGQPGLVVGN
                     DAKGVLRAKVCSTATCDLSDGLDLQVPADARANTARARLAVVGLGSGRRAVVASVPAA
                     AGTQFEAVLAAPLNGTTPKLIFSGTTGLAEGNDGVRQGRVVNISEPDEDGARHILVGE
                     AREDLDLCGRPALLAPLALNPDDLTLYPAKLQRLSEKERAAAKVVQAVRRPEAAPPAA
                     AGVLRAIGASSAIGSPAALTDGRTDTTWSENRNGVGRGEFAVFTTPADLPLVAFDLVL
                     SPPGSALDRGNVPKELWLVTQHEVFSLQFPDEAAREAGLVFRVSLPGAVSTDCVALVA
                     ESAFEERKNSRLGLAEVSVVSELTDHDPQALVGALSGGGERAKAAGALLRTLGEPAQV
                     AIARRFESLDEGGKRVALEVLDSAPCDKSVPVYLDALAGPFFLQREHARDHLRRCGRA
                     SAEALAERLSKAKGNAALAYADELVIVAPDLATRTLTHKLVNTTARERRPLRVVLARA
                     ALQPAAHDTVSQLLGDQNLPARATLDLLRALGANASMIPEARPALARVLREPSFDMHY
                     LSLGPASVLAGIEPEARAAIEHARTQIADPRLRVRALEVMPRDNSSTAGFLAALNDPE
                     VRVREAGLHGIRDARVASALPQLTKLLEDDRWPLVRRAAAEALAQLPSEAKGNDALLE
                     ALSDPAPSVRREVSAALGARGVAAAIEPLRKQLTDQAEANDVRRESARALGQLCDRPS
                     TDQLVRLASKLSDPLSSADERALGEAALEGLGTLAPADLQSRLGALLQNRGSSAAAQR
                     ALKRARIQPNCGAPGARATTSSAPDRHGSPRRAHR"
     gene            complement(19556..20446)
                     /locus_tag="VFQ61_16180"
     CDS             complement(19556..20446)
                     /locus_tag="VFQ61_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956046.1"
                     /translation="MQLAFGTPRKLPRADQLKGRVAVLDIAFAGSSGGGSFEKVTLPL
                     IQGLGARLAAWVDHHDHEQHAQYKQDPRFVLATKAEHGACPEMIDQQLVQRVGPVDTL
                     LCHTDFDGLASAAKWMRGGVEPYVGCDADARAIDTRIGEPSAVAERIDRALRARPSDT
                     GLMGLIVRHLSSGLSDGSLWEPIDRAADELIPIERETRRAAEAYQRLDPGVALVDVTQ
                     GYARIDKTLLLLLGQEREAVSIVVDRDNVSVAARFDSGLNFVTLLNLGGGMPTRVSVP
                     RGRLRDVLVALKVNPAQVGL"
     gene            20601..>21136
                     /locus_tag="VFQ61_16185"
     CDS             20601..>21136
                     /locus_tag="VFQ61_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET9956047.1"
                     /translation="MSEKPVSDPKLEPFQRALARPLSGTHFDWGTRYEGKVRDNYSCA
                     GGKRLLVVTDRISAFDRVLGTLPLKGQLLNFVSNWWFENTRDVADNHMLGTPDPNVLA
                     AVECDPLPVEMVVRAYLTGTTSTSIWVHYARGERTFCGHRLPDGLKQHQRLPELLLTP
                     TTKATKGGHDVSASREEIL"
BASE COUNT         3746 a         6878 c         6815 g         3697 t
ORIGIN      
        1 gttcttcctc gtggagagcg ccggggccag cttctcgccc ctttcgagtc aagaaaccaa
       61 cttcgacctg gcgctccgac ttgagcccgc aatctggatc ttggtggcgc cggattcgct
      121 cggagtgctc cacgatctga ccacgaccct cgccctcatg cgcgagcgcg ggcgtctccc
      181 cgactacgtt gttttgaacc aagctcgccc gccagacgcc agcaccggaa ccaacgcgga
      241 agagctcagt cgccttcgca tcgcgcagcc ggagctcgtg gttcgccgcg actcggcgga
      301 ccttgccgcg ttcgcagagc gtctctctcg gcatgcttga gcggcgatca actcccggtc
      361 ttcaagcagc gcgcgagctc cgtcagcata cggtcggcca gagccaagcc atctgcgaac
      421 gtttcgttct ccaatgggcg cctcgtcagt cgcccatccg gggttagccg gtagggggac
      481 ttcttctgag ccacgagctc acccactttc accgggtcca gggtcgtatc gtcgcgcaga
      541 tgcagcgaga cgctgcgctc actcgcttca cagccgagca cgcgaagctt ccgaagctcc
      601 gtctttaggc gcatcagctc gacgaagcgg cgtgcttcga ccggcggctc tccgaagcga
      661 tcctgtagct cgctcgcgat gtccgctacc tctgcctcgt cggaggcgct cgcgagccgt
      721 ttgtagaagc tcaagcgaac gcccacgtcg gacacgtagt tgtctggaat gagtgcatcc
      781 acgtcgaggc tcaattccgg atctacctcg tggaccaccg gttccccgcg cagctcgtgc
      841 gtcgcctcct cgagcatgtg acagaaaaga tcgaatccaa cgctcgccgc aaagccactc
      901 tgctcggccc ccagtacatc gccggccccg cggagttcca tgtcgagcgc cgcgatgtgg
      961 atgccggagc ccagctcaga atgccgttcg agggcttcca gccgagcacg cgactcatca
     1021 gtgagctggc tcgggggcgg caccaagagg tagcaatagg cgcgctcgct gcttcgcccg
     1081 acccgccctc tcaattgata caactgggcg agcccgaata agtccgcacg gtcgacgatg
     1141 atcgtgttgg cgcggggaat atccaagccg ctctcgatga tcgccgtggc gaccaggatg
     1201 tcgtactccc ccgccacgaa gtccaacatc gtccgctcca gcacgtcctc ggtcagcttg
     1261 ccatgcccga ccgcaatgcg tgcgcggggc accagcgcct gcagccgctc ggcgcgctcg
     1321 taaatgccct cgacccggtt gtacacgtaa aacacctgac cgccgcgctc gagctcgcgc
     1381 tcgacggctt ggcgcaccag ttccgggtcg aattgcgagg tgatggtgcg aatggcgcgt
     1441 cgatcgaccg gcggggtgct gatgatggac atgtcgcgca gcccacccac agcgagctga
     1501 agcgttcgcg ggatcggtgt agcgctcagg gtcaggacgt cgaccgacgt tcgcagctgc
     1561 ttgatgcgtt ccttgtgagt cacgccgaac cgctgctcct catccaccac gagcaagccg
     1621 aggtgcttga aatgcacgtc cttcgagagc agtcgatgcg tgcccacgac gatgtcgacg
     1681 ctgccgtcct tcaagccctt gagggtttcc acctggtcag cgcgagactg gaaccggctc
     1741 atggctttga cgcgaatcgg gtagtcctgc aggcgctggg agaacgtcat gtagtgctgc
     1801 tgggcgagca ccgtggtcgg gcagagcaac gcgacctgtc gcccggagtt ggcgcacagg
     1861 aacgcggcgc gcaaagcgac ctccgtctta ccgaagccaa cgtcaccgca cacgacccgg
     1921 tccatgacgc gctcgctcgc gagatctttg agaacgtctt ggatcgccgc ggtctggtcg
     1981 cgcgtttcgt cgtaaggaaa gctcgcctcg aaagcggcat aatcgtcatc gggcgcaggc
     2041 aaggcgtcct tctggagcgc gccccgttcg gcgtacaaac gcagaagctc atcggccatc
     2101 tggcggacgc gttgctggac cttggccttg gtcttggaga aggtctggcc ccccaagcga
     2161 tcgagcttgg gctgggcatc gccacccgag aacttctcga tctgattgag gcgatatacc
     2221 gggaggaaga gcttgtcccc acctccgtac tcgacgacga tcaagtcgac cgaaacgtca
     2281 cccacctggc ggcgttcgag cccaacatat cgcccaattc cgtggtcgac gtgcaccacg
     2341 taatctccgg gttccagcgc ccgcaagtcc tccaacatcg cccttgcagc gcgcggcttg
     2401 gcgggggctc ggtgcgttcg ttgaccgaag atctcctcct cggtcacgat cacgaagcgc
     2461 tccgcggggc agatcgcgcc gcgggccatg ggcgccactg caaccaggac agagctaagc
     2521 tgcgagtcgt tcgctgtatc gggatcccac agcgctacct tcactccgcg atggctcaac
     2581 agtgcggcaa cccgttcggc ctgagttctg gctcgcgcag tgataatcac gctcaatccg
     2641 gcgtcttgcc aaagtgtgat acgggtgatc accggatcta gcgaggcggc cttcccgcga
     2701 gtcgttcgcg caagtttgcc ttcccgggtg aggtcgccgt gatctcgaat ccacacggag
     2761 ggggcatctg ccgacccgcc ttcgagtgcg tccaagcccg tggtggctgt cgatccggcg
     2821 accaaagagg catgcgccgc caagcacgcg cgctgctcta aagcgcgagc gatttgcgat
     2881 tcgtcggtat agagctgttc gaatccgaag tgcggagtct gcacgcgctg gccttccccc
     2941 tcgcgacctt gtgctgcgtc ctcgcgcaaa gcccgaacca cggccggcgg gtcgtcgatc
     3001 acgacgaccg ccttttcgcc gaagtaatcc aaaagcggaa ccgtctcgtg gaaggcgggc
     3061 agatagccct ctaggccgaa atatgcgtgg cccgttgcta gatcctcgat gaaggcgcgg
     3121 gccttcgtag acgggtagtc gactcgatcg cacagatcgc ggagcacatt ccgtgcgcgg
     3181 gactcggccg cggcatcgat gatggcctcg cgtgccgggg gcaaccacgc ctccgtcagt
     3241 tcgccaatcg tgcgctggtc gtcagggtcg aatttgcgaa tcgttagtat caagtccccg
     3301 tacagctcga cccgcagtgg tccttcggcg cccgccggcc atacatcgag taagccgcca
     3361 cgcaccgcga aggtgcccgg atcctccacc accggcgttc tcagataacc ggcgaaagtg
     3421 agccggctag cgagctgcgc cagatcgagc tcgctctccg cctcgatgcg aatcgccgcc
     3481 tgccgcagca cttcggggcg caccacgcgt cggcccaggg cgctcgcagt gattacgacg
     3541 aattggaaag gcaagcccgc cgcgatgtga aaaagagtcg cagcccgcgc cattgcggca
     3601 cgacggtcgc cgtggacctc tgcatatggg gaagtctcgg ttgctgcgaa catcagtggg
     3661 cccacgctct gagccgcccg ggtcgacccg ggcgcgacac ggctgctcgc gggaagacct
     3721 cgactcaacg ccgataggtc ggcgctggcc cgtcgagctc ggtccaagtc cggtgccacg
     3781 tagacgacgt gtttcgcgcc ggagtcacac agcgcctgtg cacacaacgc cggcgcggct
     3841 ccgcttgcgt cccggacgtg caagatccct cttcgttgga gaacttgagc cgagagttcg
     3901 cgcagggtga ggatcctgcg atcctcaggt agagcgagag aaagagctgg attggcgagt
     3961 gagtcagcgg ccgacatgcg tcggcggagt gttactcctc ctcagggatt cgcgcgaggg
     4021 cggccaacgc gtagttgcgc gcgcgcacca cccacgggcc atcggggcgc cgcttcaaga
     4081 aactctgaaa cgcctcgaac gcggcctctg ggcgattcaa ctcgagcagc aaaactcccc
     4141 gggcgacctc ccccggatag tccgggtcga gcttcgccag ggcttgggcg tactcgagcc
     4201 tgcgggcggc gggcgcgtgc gcctcgtcgt cagcgttttc taggagcaac cgatagtaca
     4261 aacgaaattc ctccagccca gggcggaacg gagtgcgctg gagcaacccg gtgagctcgg
     4321 tccagtgcac gcggtagaga atgaatagct gatctgccga gagtcgcagg cggccgttct
     4381 cgtcaagcca accattggac gtacagctcg cgacgaactc accaccgagc tcgcggagat
     4441 ccgagtccag ctcttgggcg tcgagccgcg gcctcccgtg tgagccgcgc cagcgtgcga
     4501 cggcggaaag aaacagatcg ctctgcagag ccctcaatcg caagagctcg gcctgacccg
     4561 tttgcggatc cgagcgcagc acgtttcgga cggaactcac cagttcagcg cgcgcttccg
     4621 cggcgcgcgt aggattgtcg ctgaaggtcg ctgagccata gcgcctcatc cactcaccca
     4681 ccaggcgtac cgagaagggc agggctttcg ttcctaccgt cgctgcgcgc tctcggtcag
     4741 ccgcttgctc tcgaatgatc accgctcggt cgggcagagg taagggaacg tgctcactgt
     4801 ggaccgcgcg agggagcgcc aacgcgactg cgagcagagg gaacgcgacg gccaatgcgg
     4861 ccaggggcca tccggcgaga tgccgtcgta atccaaggtg cgtgtcagga gaatgggtca
     4921 gaccttggct catgcgaccg tgacggatgc gaccctggtt catgtgacac tcacgggctg
     4981 ctactccctt gatcgaaagt ttccctccaa cattgcctct tccgaaatgg aggcgtacga
     5041 aatagttcgt gcaggagaaa cgcggtcatc cccgggttcc gatccaattg cgcgccactt
     5101 gccatcgtgc cagtggagga tcgttcgtgg cgcaagtagt caacgtgagt gtcggtggca
     5161 tgttcctcaa ctcggccgaa gtgctgcagt tcggtgaaga gttgaccatc gagctcgaac
     5221 tgccgcgagc gcgagctccg ctgcgactgc cggcgatcgt aagatggacg ctggcggagg
     5281 gatttggcgt gcagttcggt ttgctgggcg cgcgagacac ccacgcgctg gtggagctga
     5341 cccggggttg aagggaagag cggcgggagc ggcccgcgca agtggggcac tcgagcgccc
     5401 tggctccgcg atttcgagtg ccgggctccg agggaccgcg cgacaggcga tccgaactcg
     5461 gctccagcgg gcggacatgc tagcgaatcg gtagctagct catgtcgacc attccgcgtt
     5521 cccctcagga tttcttccag atctggttgc cgagcgcctt cggtccagtc gcggcaaagc
     5581 ccagcggttc ggggcaaact tctgcaccgc cgaactcgcc tggggcgctg gtgttttgga
     5641 tcggagacga ggccccgttc gccctccgcc tcagcgcggg caagctccgc gtcgagcgag
     5701 ctctggcacc ggactgcgtg gttcaagtca tcctcagtga ggccgacttc gagccggtga
     5761 tcgtgcgcgg cgcggaagcc ctcatggcgc aaggcaatcc tgagaaacag ctctccgtgc
     5821 tccacgcctt gagtttggac aaggaacgag tcgagctgat ccgcggcgtg ccgggaagcg
     5881 tcgccttcgt gctccccgcg ggcgcggtgg agcaccgtat cgtgctcacc ccgggcgtgc
     5941 gtccgaaagc gttgggcccc accgagtgta cggtgcgctg cgcactcaac gacttcctcg
     6001 ggatgcaacg cggcgaggcc aacccgttcg atttgatgat gaacggacgc ctccagatta
     6061 gcggggatgc tcagatcccg atggcactca gctctcttct gatgtgaacg cgggcgacgt
     6121 gaacgcgagc acaccgcagg cggcgtcgcg tgcgggtcgc ggcgtgccgc cgcggctgtg
     6181 tgcgctctcg gtcgacctgg acgagatcac tcactatcac gcaattcatg ggctaagggc
     6241 gccagagccg gcctgcgcga acctggtcta tgatctggcg atcgcccgct gctccgagtt
     6301 cgcgcgcgac ttcgaagtgc cggtgacgtg gttcgtggtg gcggcggatc tggcgcgtag
     6361 cgcgaacgcg aagcgcgtac gcgagctcgc ttccgcgggc cacgagattg gcaatcatag
     6421 cctcactcat ccctatgaac tggtgcgact gacgccctcc gagcaatggc gacagatcga
     6481 cggggcgctc gagctgtttc aacacgagct cgggtaccgc cctcgcggat tccgcgcacc
     6541 gggctacagt gtcaacgacg cgctcctcac gatggttgcg aaggccggtc acctttatga
     6601 ctcttcggtg ttcccgagcg cgccctacta tgcggccaaa gccttggcgc tgctcgccct
     6661 gcgcctgcga ggtcggcact cgaaagcaat cctggactca ccggagctgc tgactgcgcc
     6721 gcgacgtcct tatcgaatcg gtacgccgta cacgacgcgg ggcgcgggcc tattggagct
     6781 tcccgtacaa gtcacccgcg gcccgcggct gcccttcatc ggcaccgcac tggtgctggc
     6841 gggtgaactc ggtgcgcgcc tgctgaccga gctagtgatt ggtgagcctt tcatcaacct
     6901 ggagctgcac ggtatcgact ggcttggaga tggagatcct ctcgctcccc tgcgtggttt
     6961 tcaaccggat gtgcggatcg agcaccagcg caagcgggcg atctttggag gcgtgatcgc
     7021 tcgcatgcga gccgccgggt accggttcgt cacgttatcc gatgcagctc gccaattcgc
     7081 gcatctctaa acaagtgttc cgcagagtca gagcgtgtcg tggattgaat tcaacagctt
     7141 cgcaaaataa cgctctcgtt gcgcgtcgaa agacttctct gcctgctcca tgcctcgtca
     7201 gtcgaccact ccatctggca cgcgctgcgc ggccgggtta tggcgttcaa gcaaaagtgg
     7261 tctgtggctc tcgctgcgcc gccggtggcg ttgaggagat gggattgatg actcgggttt
     7321 ggtttagctt agtttcgagc gttgggctgt tggcgctgaa cgtggccggt tgcgcgagcg
     7381 aggcgcccga tgaaccgcag gacccccagt cggtgtgcca gcgcttttgc gcgaacacgc
     7441 gccagttgtc ctgtcccaac gtgaatctgt gcgtgtcgca atgcttggac ggacgtaaag
     7501 ccgtttcccc aaactgcctt ccgttgacag acgcgacgat gcgctgctac gcggcgcgcc
     7561 cggctacgga cttctactgc gacccgatcc tcagtaagcc cgttccgttg ccgggcgtct
     7621 gcgcggacga agtggccgcc cagacgagct gcgcgactgg caggtgacgc gcaacccatg
     7681 ggtgacggag gtcgttccga ctcgacgggc gaagccaagc tagatcacgg cttcgcccat
     7741 cgcggatcac ggccagcagc gggattcaag gcgcgtttgg cacgggaggc agagctgccc
     7801 aacgcagcgc ttcgccggtc tggctcgaga tctgctgcgt cttccagcgc ttcatcgaca
     7861 tgagatagac gccaggctgc ttgttgcgcg tggagaagaa cgccagcatg cgaccgtcgg
     7921 ggctacatgc gggtgagtag ttcgagccct gatcctgcgt cagccgcgcg atgccgcggc
     7981 cgtcctcggc ggagattacg agatcctgac ggtctccccc caccgcgacc gcgtaaacca
     8041 ggcggattcc atcttccgtg tcacaaaaag tcgggcccga ggccgtgaag ccagccggtg
     8101 acacaggctt accatcgagg aaaacgcgct gcgagccttg cttggcgtcc ccaccgatcc
     8161 aggcgagctt gccacttggg ctgaatacgg gatgcgtcgc gagctgactc ttggagaccc
     8221 gctgcatctg gcttccatcg ctggagccca cgaagattgt gctttcgccg ttgtcgctgc
     8281 tgaccgccaa cgccatgcgc gcgccggcct tatcgaagct caccccgtag agtgacgtcg
     8341 tgaacgggat cgggacgggc ttctccgtag tgccagatag agtgaacagc ttgtagggcg
     8401 cgtaatctcg gctcaacaag tagtacaggg aatgcccggg gccccaggtc ggtgcgatgg
     8461 cgtgatcgtt cgcgttggtg agcggggtca gcccattacc gtcggcgtcc acccgaaaaa
     8521 tccgtcgact gcgcgcccag cgtgcggaaa atgtgagctc gctcgcgaag ccgccggggc
     8581 gtcccgtcag cgcgccgagc aaagcgtccg tgatgcgatg cgcggtgacg cgtgcgtctg
     8641 ccttcgagac cacgatcttc ttctgataaa cgggctcttt gccgacgttc aggaagtagg
     8701 caatccctag aacttgaatc tttcctggag tggcgtcggc gcgtgccgcc accttgacga
     8761 tggcttcagc gcctaggttg cgccacgcgg gaacgtcaat cgcatcgtcg aagccgtaca
     8821 agcccacggg cgctttggcg tccggaatca agtcgaatag gcccgtcaac tcgaagtcgc
     8881 gtcgcaccac gcccctcacg atcacgtctt cgagatcggg cgagagcgag gggagtatcg
     8941 cgatccgtgg cagtctttct tgttgagtgc cggtgacgac cagctcaccc aaagcctgct
     9001 cgtcggcagg agctgaggcc ggggccgcgt cttgggcgtt cgcggtcggc gcggccagag
     9061 caaagctcag ggctccgccg gcgagggcgg ttcggagggt ggcgcgggct cggaaggtgg
     9121 cggaggagca ggcgccggtg ccgcgggtga ttcgggcgaa ctgggcggct ggggtgaggg
     9181 attcggtgcg gtgtcgttgc attgcgcgga ccttccggag aagctgacgg gctgagtggt
     9241 gcccaaaatg tcgggataaa gcggaggggg aggtggcagc tcctcgccca cgatgcggtc
     9301 catcgtgctg cgggtgcgtt cgtcgaagat actgttgcca ctcgggctca cgacttcata
     9361 cgaagtaact cgccggtcct gccctacggc aacgacgacc cggctcgtga gctttttgag
     9421 ctcggcgcaa ggaatctctc cggtgggtgg tcggaatcgt gcgttgaacc aggccaagat
     9481 cttggcgcgg tatgcgctga cggcgcgcgc cttgagcgga tcggtctcgg tgccttcttt
     9541 gacaccgtcg ggagagcctt cgccctcgac cgttggtgcg gcgtcagggc ccgcatccag
     9601 cagaacctga tcgacttgct tggcgacctc cgcgtccggc gggggtgccg aggcgtccgg
     9661 tttcggcagc ggcgtgctcg ggatggcgtc cggcgtcttg gaggccttcg tagatggcgc
     9721 actcgtcgct tcataacgct gcaccggcgg gttcttcttc cacatctcgg gcaacttcgg
     9781 acgaaccttc ttcccgccga gcttcaacag cggcagctcg tccaacaccg ggcgcacggc
     9841 aatgggaatg gtggacggag cgggtggcgg ggcggctgga acgtgagact tggactgccc
     9901 ggccaacacg agcagcgtga aaaagccgga cagaaccgcg gcgctgaacg cgatggcgag
     9961 cgccacttcg tggggctcga aagttcgatc gagaggggac gcggacgacg acatcgagcg
    10021 ctcagcgctt ccgattgggc ggggccgaag gggcaacacc ggacgcggct gcgtccggca
    10081 cttcttcgac atcgggctcg accaaaagat tcagcgcttc gacgccacta ttgcgggcag
    10141 cggccaccac ctccgccacg atgccatacc gggcgctctt gtccgcgcgg atgtaaagct
    10201 cgcgctcctt ctgaacgcgc tgattttctc gcagcgcggc gtccacgcgg cccgtcacgt
    10261 cctcttcgcc gaataggacg cgctcctctt tcgtgatgct gacgaggagg cgtgcgtcct
    10321 tgatgggggt ttgcgccgcc tttacttcgg gtaagtcgat gcgcagcccc gtcgtaagca
    10381 ggggggccgt caccatgaag accacgagta ggaccaacat cacgtccact agtggcgtga
    10441 cgttgatgtc gcggaaccct ccgcggcgac ggctaccaac ctgcattccc atggctcacc
    10501 tgctgttgcg ggcgcggcca taaggcgacg cgcgctcttc ttctgccaac gtctccaccc
    10561 aaacgtccga gctcgccgcg agctcgtcca tgagatcgga gatgcgtttg tctacgtagt
    10621 tgtagccaat cgttgctggg atggccgcga ccaagccgaa cgcggttgcg atcagagcct
    10681 cgccgatcgc cggcgcgacg acgggcaatg acgcactctt ctccacgccg atccgcaaga
    10741 aggcgtccat gatgccccaa acggttccga acaagcccac gaacggtgcc gcggaagcaa
    10801 tgctcgacag ggtcggcatc aacacggttg cgcgttgttg ctcggaggtg agcgcccggc
    10861 gagccaccgc accgagcagc tcggcgccac ccgcggccgc ctctctgcga cgggccaggg
    10921 cgatcacaat ccgaccgccg ggtgacgtct tgtagcgctc tgcgagcgac aacagatcgg
    10981 acgctcgcga gacgccttcg gcggcgcgct cgaagctccg gagggcggca cggagccgag
    11041 caatttgggc gaacttgagg aaccaaatca gccaaaccaa ggctgccgcc acgatcaaga
    11101 gcaccaagac cacgagcact acgcccgagg agccgagcat gagcgccaaa ggatcgatgg
    11161 ccttgggagg tgccgcctcc ggctgcgcag tcatcgccag catcaatcag cttccgagca
    11221 aaacgtcgac gcgacggtct ttggcccagc tggcttcctc ggtgcccacg gcgtcgtctt
    11281 cgccccgaga cgtggtctcc atccgcgagc tctccagccc cgccgtggtg atcgcgccct
    11341 tgacgttgtc cgcgcgtcgc tggccgagca cgcggttgta ctcgtcatcc cctcgcggat
    11401 ccgcgtggcc gaccaggcgc atacggcggc ccttgagagg gcccgtcgtg aagcaatcgg
    11461 ccagcttctt caggacggcg cggtcctggg cacggatatt cgcagagtcg aatgcgaagt
    11521 aagcctccgc atcagagatt ccgcacgcct tcttgatctc gtcggagatg acgaggttcg
    11581 actgagtggg gttatcactc ggcttggggt tggccggttg ctcagcagcc ggcggcgctg
    11641 gcggcggagg cggaacggga gaacgcgctg gcggcggtgg gtcggaaccg caagcagcga
    11701 gcgcggcgca cgacacgaac agggctaagg caacagttct cacgacatct ctcctttggt
    11761 aatttctgag cgacgccgat cacagggacg gcatcgaaga cacatccgca gtgcctgccg
    11821 cggaaagctc cgcgcgtcta taacacacgt ggcaggtccg gcgcgccttc actgtgggcc
    11881 acgcgaccag gcctggaatg cctgccgcgg tgatgaacgc gccactggac tggagattcc
    11941 cgcctgcagg ggaaacggcc gcggactgtc agcgggagcc gaccgttcgc gcgcgcgacg
    12001 aacgaccacc gataggtcag cgcgtagcac ggagataggc caaaaggccg atgatcccga
    12061 gcaggaacac gaaggagatc agcacatacc cgctgcggcg cggattgacc ggtcgcggct
    12121 cggtgcgcgt cgtttcgagc tgagtgagcg caagcagggc gacggccttt agtttcttct
    12181 ccgccaactc cgcgcgcgca cgatcaccag tcatgccccg gtagcgaacg gctgcctcga
    12241 ccaggcgccc gctgagacgg cagtagtcta ggaacccggt gtgcgcctgc tcctcgtccc
    12301 agtggtccag cacgcgcttc catgccgctt cgagtacggg atccgcgggg ttcacggcag
    12361 gcctccgtta gcgtcaactc gattagccga tgcgaagctc tctcagcagc cggcgagtca
    12421 acgcgaagta ctctgcacgc gagtacggct cattcaagat gtggaaatct ttgttggcaa
    12481 gccccacaca cccaaagcaa tacgtgcagg ccgagagccc caccgaacgc acgagatagc
    12541 tactcgacga gcagttctcg cattccacgc agtgctggca gccgattagc ccgcgcgagc
    12601 cgcgacagtg ctcacaatcc gtgcagccct ggcagcgcac gcaaaaatgg cagcgcagca
    12661 aacgctctga gtccgagcaa aacgtgcagg cgctgcagcc ttggcagcgc tcgcattcca
    12721 cacagcccgt gttcccgcgt tcgcgaccca cttgagaaag aaggtcgcgg tagcgcgcgt
    12781 cgaaggtgcg ggcatcgagt ggctcggcca tcgccgcgac tatttcaccg ctgcggcccg
    12841 aggggcaacc gaaactggcg agcgtgatcg ccccattact cgaaccgagc gccccgttct
    12901 cggcacctga gcgcttccaa tggcatacac cggagcgggg ctgtactagg ctcgggcgag
    12961 cgcgatgttc ggacgactca tcttcgtggt tggcgtggcg tctgcctgtc agccggtggc
    13021 gccagccgct ggtccaggca acgcgaacca atccgcggct tcggagcgtg caccagctcg
    13081 gcccgcccga tccgccgtgg gttcagcgga cccggagtgg gaaagtggtt ggagcaagcg
    13141 ctggctggcg cggcaagcat tgacgctcga gctgccggat gcggcccact ggcgaaaggg
    13201 caccaaagtt aggtccttcg tgatcgagca cccggcgagc cattccgttt tgaaggtgga
    13261 atccgagcgt gcggaacgct tggtgcgacc tgccgactgc gagcagcggg cacgcttggc
    13321 ttggcccgag gcgccacgtg gggatgatgc gtcacgggtc gacgctcgtc gtctcgatgt
    13381 gccgccaggc tatgtgactc aggtggaggt gctcgtgctg ccgttgcagg aagatacggc
    13441 cgaaggccac gtgctggcgt tcggtgcggc agtgggccgt tgtctcgcgt tgcacttcac
    13501 gactcgggcc tccggccccg agcgcttcga gcggatcgca gaacgtttgg gcttggtgac
    13561 agggcgcttg ctgccttcaa tcaccccgct cagcgcggat gatcgcgtgc agaccgagcc
    13621 gctcgaccgc tgatgcgagc cttcgagtcg gcttcggaat tcgatcgtgc cagggccggt
    13681 cagtgcagcg tatgccactg cccttcgtgc tgaatgcggg gaacctccgc ttcgaggttg
    13741 ggaggcggca ttcctaccgg cgcgccaggg ggcgcgctct gcgtcaaaac gatgtgaacg
    13801 cgtgggggag gcgagctcgc cgcctgcctc atccgcactg catcttcacg cgcctcttct
    13861 gcggcccagc gcgcaagcgt cgcctggctg cgcgatcgtc gtttgcgcca ggcccacagg
    13921 aatagcccga gcacgcccat ccaaactacg ctgccgctca acagcacggg ccaaaaggtg
    13981 tagcgcttgg ccgcgtcgtc tcgccactct tgctcgagcg tgaacatgtc cgcgccgtag
    14041 gcatctcgga gcgcggcctg gaaatcttgc cccgcgcgca gacgttccac caagccttgg
    14101 aatcggaact gctcacgctt gcggaccagg aaccgaacta cgtccaccgc ttgggcataa
    14161 gcgatttcgg cgtcgctctc gtgggctggg aagccctcgt cgagtttctc caacgggatc
    14221 agcttgtccg agagcgtggc catccacagc gtctgcagcc ggttcagcga agtttcaccc
    14281 gacacgaaca cggcgaaacc ttcatcgaac cagcgcggga cgtgcgcgcc attcacggcc
    14341 tcgtgcaaac ctacgtgtgc aagttcgtgg cggaatacct gcaccaaatc gtgctgcgaa
    14401 ttgggatgga gcggggccac gctgagcagg gtcaagccga tctgcggata ggcgacgccc
    14461 gctgcgtagt cgggataggg cgcctcgggc ggcgccaacg tcgccatctc gcctggcgta
    14521 cgcgccaggt ccacgcgcac ctgagtcagg accggcacgc ccaagcgttc tgcgagctcg
    14581 gagcgaaacg gcaccgcttc gctgatcaag gcctgcaggc gttctcgtgt ttgcgggtgg
    14641 tagtgaaagc gaatccagcc accatcgtag gtgttgaacc cgtcgggcaa gcgcgtggcg
    14701 ttcaagcgcc cgggcgctaa gcgtggcgca tcttgcggtg aagccggcgc gtcactcgag
    14761 tctacgcttc cagtggtccc ggtgcgctcg gtgctgagcg cgggcgtctc tgcgcgcgcg
    14821 gagctcaggt ttccgacgag ctgtgcgctc cagaacagca ccgcacacaa ggcgagccgc
    14881 aaaagccgga gggatgcgag cttttcccac gagcgcaacg gcggcagcat cgtcctggaa
    14941 tatggaccga tttcagggcg tctgccgctc gccggttggg gtaaccctgc cccgccaagc
    15001 aggtaggacg gattagacca accgcgccac ccggctgaaa tgaacccgct cgagccaaac
    15061 cgggagggct gtgcgtgggg cgggtgcttc caagagccag cgccacgcgg aggcgagcaa
    15121 atcgtgggct tccgagggtg cgggctgagc gcccagagta ctgaccacac cgaccaccaa
    15181 ggtcagcagc cacgcgacgc cgccaatccg gctgagcgcg agcggcagcg ccccccatcg
    15241 caaagcaagg ccggcggcgc ccagtccgat ggctgcgtcg agggcaccgg aacgagtcgg
    15301 cggagcgccg agcaacttaa accacaagag caggaccgct gaggcggctg ccacatcgag
    15361 cgctcgcgtc agcaagttcc tcaccttggg tgagcgacgt gaacagaatg acagccagcg
    15421 tgcggccacc acggccgccg ccaaactgac caccaccgcc ccgaccgcga aggtgacggc
    15481 tccgagcggg cggtgatgcg tcgccttctt gaggatttgc ccaaagagac tgagcggtac
    15541 ggccgcaagc gcaatgccgc aaaggacggc atgagcgcgg cgcgacaggg gttgggccag
    15601 gttccaggcg ccgagcagag aaccgaatat cagcgcgccg ccggccgcaa tctccgacag
    15661 gcgcacgaga ggttctccgc gcaacgcacc gcaggcggcg ctagccagcg caccgagcca
    15721 cgccgcactc aaaactcggt cgaacctcaa accggtcgga gctcgagccg aagccgattg
    15781 gcgagtggct gctcgatgaa gccttgggtc aagtagaagc gcagcgatcg tgcgagcgtc
    15841 tcgcgagcgg ggcggaaact gtagccgagc tcgcgctcgg ccttcgcact cgagacccaa
    15901 acgcgcgagt aagcgtagtc cctcgccagg cgataggtca gcagtgggtc acggcccgcc
    15961 cagcgcgcag agaattccat gaagcgagcc accaattgca gtaggcctgg gctcggggtc
    16021 gggccgggtt cactgagccc ggtcagctca tgcagcagcg tgaagaagtc tcgataggtc
    16081 aggttctcgc cgcccaagat gtagcgctct cccacgcgac ctttggccat cgccaacaag
    16141 tgcccgcgcg cgacgtcctc tacatccacg aaggagttcc ctccgtccgt ggtcggagtc
    16201 tttcggttgg ggggcgtctt gacgtagtgc aatagacctt gcccgttgtg agtcggcttc
    16261 caatcaccgg gcccgaagat cgcccccggg agcacgctga tgattggcat tcccgctttg
    16321 acgtgctcct gcacgactcg gtcggccgca atcttcgccc gtacgtacgg ctctggatcc
    16381 tggagttggt tctcgctgct ctcgtccaac agctgatcac cttgcgacac gccgagcacg
    16441 gcggtgctac tcgtaacaac gattctgggc agctcgtgtt gcttggccgc cgccagcacg
    16501 gcttgggttc cgagctcggc cgagcgcagc acgtcccgct gatgttgccg accatacttg
    16561 taggtcgaag caacgtggaa taggcggtca caaccgatca acgcacggta cacgctggct
    16621 tcgatggtca cgtcgccgta agccagcgtg cacaggtcac gcggcaagtt ctcgaggact
    16681 ttcaggttgg ctcccggtcg cacgaaggct ttgacgggaa cgccattctc tacgagctgc
    16741 cgaacgaccg tcgcacccaa gaagccggcg gcgccggtca cgagagtcca cttcttcttc
    16801 attgcgctcg cctagttacc aaacaccgca ttccgagcaa agccgcgcgc gaccggggct
    16861 gcggtttctg cagaatttct gccgaaagcg tgcagcgcga acaaccccag ataggcgctc
    16921 gggtcgggag ggggctcgcc cgccataagg ctcaacggtg ggctctcctt ggcgatccgt
    16981 gacggtcggg agcggagctg gttgtcgccc gcgctcctgg agccccgcag ttcggctgga
    17041 tgcgggctcg tttcaacgct cgttgggccg ctgcggaaga gccgcgattt tgcaaaagcg
    17101 cgccaagtct cgattggaga tccgccggcg cgagggttcc cagcccctcc agcgcggctt
    17161 cgcccaatgc ccgttcgtcc gcgctggaga gcgggtcgct gagcttgctc gccaatctaa
    17221 cgagttggtc agtgctgggc ctgtcgcaca gctggcccag cgcacgagcg ctctccctcc
    17281 ttacgtcgtt ggcctcggcc tgatccgtga gctgcttgcg cagcggttcg atcgccgccg
    17341 ccacgccgcg tgctcccagc gcggcgctga cctcccgcct tacggatggt gccggatcgg
    17401 agagcgcttc gagcaaagcg tcgttgcctt tcgcttccga cgggagctgg gcgagcgcct
    17461 cggccgcggc gcggcgcacc aaaggccaac ggtcgtcctc gagaagcttc gtcaactgtg
    17521 gcagggccga ggccactcgc gcgtcgcgta tgccatgcaa gcccgcttcc cgcacccgca
    17581 cttccgggtc gttcagagcc gctaggaatc cggccgttga cgagttgtct cggggcatca
    17641 cttccagcgc gcgcacgcgg agccgtggat ccgcgatttg ggtgcgcgca tgttcgatcg
    17701 ccgcgcgcgc ttccggctcg atccccgcca gaacgctggc ggggcccagc gaaagatagt
    17761 gcatgtcaaa acttggctcg cgcaaaaccc gagccaaggc cggccgcgct tcgggaatca
    17821 tcgacgcatt tgctccgagc gcgcgcaata aatccaacgt ggctcgtgcg ggtagattct
    17881 ggtctccgag cagctggctc accgtatcgt gcgcggctgg ttgcaaggcg gcgcgggcga
    17941 gaacgacgcg caaggggcgg cgttctcggg cagtggtatt gacgagcttg tgagtcagcg
    18001 tgcgagtcgc caagtcgggc gccacgatca cgagctcgtc cgcgtaggcg agggccgcgt
    18061 tgcctttcgc tttgctcaat cgctcggcca gggcctcggc gctggcgcgt ccacagcgtc
    18121 gcagatgatc ccgcgcgtgc tcacgctgca aaaagaacgg cccggcgagc gcgtccaaat
    18181 agactggcac actcttgtcg caaggcgcgc tatcgagcac ctccagagcg actcgcttac
    18241 cgccttcgtc cagtgactcg aagcgccgcg caatcgctac ttgcgcgggt tcacccaacg
    18301 tcctcagcag agcccctgcg gctttggcgc gctcgccccc gccggagagc gcgccaacca
    18361 acgcttgcgg gtcgtggtcg gtgagctcgc tgacgacgct gacctccgcc aagccgagcc
    18421 gcgaattctt gcgctcttcg aatgcgcttt ctgcgaccaa ggcgacgcag tccgtgctga
    18481 ccgcacccgg tagggaaact cgaaagacaa gacccgcctc gcgcgccgct tcgtctggga
    18541 actgcaacga gaagacctcg tgctgcgtca ctagccacag ctccttcgga acgttgccgc
    18601 gatccagggc gctgccgggc gggctcagca ccaaatcgaa cgcgaccagc ggcaggtccg
    18661 ccggtgtcgt gaacaccgcg aattcgccgc gacccacgcc gttgcggttc tccgaccacg
    18721 tcgtgtcagt ccgcccatcg gtgagcgccg ccggtgaacc gatcgcgctg ctcgcgccga
    18781 ttgcgcgcaa aacccccgcc gccgctggcg gcgcggcctc gggccggcgt acggcttgaa
    18841 ccaccttcgc ggcggcgcgt tctttctcgg agaggcgctg cagtttggcg ggatatagcg
    18901 tcaggtcatc ggggttgagt gccaggggcg cgagcagcgc gggccgtccg cacaagtcga
    18961 gatcctcgcg cgcttcgccg acgaggatat gccgagcgcc atcttcatcg ggctcggaga
    19021 tgttcaccac gcgcccctgc cgcaccccgt cgttgccctc cgcgagcccg gtggtgcccg
    19081 agaagatgag cttgggcgtg gtgccgttga gcggcgcagc cagcactgct tcgaactgcg
    19141 tgccggccgc tgcgggcaca ctcgcaacta ccgcccgacg accgctgccc aatccgacga
    19201 ccgcaaggcg agcgcgcgcc gtgtttgctc gcgcatcagc cggcacctgc aaatcgagcc
    19261 catcactcaa atcgcaagtg gcggtgctgc acaccttggc tctcaaaacg cctttggcgt
    19321 cattccccac gacaaggccc ggctgccccc tccctggggg cgcgaacgcg ggagccgcca
    19381 acgcaacttg accgaaaaag ctcaccccga gagccgccag gatccgagct gccaaccgat
    19441 tggagaagcg catgcgcggc gaagcgtgtc agccaatccc ggagaaggga agggccgcca
    19501 tgcattcccc gggcaccttg accgtgttcg cgcgtcggga atccgccagg gccgcttaca
    19561 gcccgacctg cgcgggattg accttcaacg caaccaacac gtcgcgcagc cgtccgcgtg
    19621 gcactgaaac gcgggtcggc atgcctccgc ccaagttcag caacgtgacg aagttgagcc
    19681 ccgagtcgaa acgtgcggca acgctgacat tgtcccggtc gaccacgatg ctgaccgcct
    19741 ctcgctcttg cccgagcagg agcagcagag tcttatctat ccgggcgtag ccttgcgtga
    19801 catcgaccag cgcgaccccg ggatccaagc gttgataggc ttccgcggcg cgccgcgtct
    19861 ctcgctcgat gggaatgagc tcgtcagcgg cgcggtcgat cggctcccag agtgagccat
    19921 cggacaggcc actgctcagg tgtcgaacga tcaagcccat caagccggta tcggagggcc
    19981 tcgcccggag ggctcgatcg atacgttcgg cgactgcact cggctcgccg atacgcgtat
    20041 cgatcgcgcg agcatcggcg tcacaaccta cgtagggctc tacccctccg cgcatccact
    20101 tggcggcgct cgccaagcca tcgaagtcgg tgtggcacag taacgtgtcc accggcccta
    20161 cccgctgcac cagctgttga tcgatcattt ctggacacgc gccgtgctcg gccttggttg
    20221 ccaagacgaa gcgtggatcc tgtttgtatt gcgcgtgctg ctcgtggtcg tggtgatcga
    20281 cccaagccgc gagtcgcgct cccaaacctt gaatgagcgg caaggtcacc ttttcgaagc
    20341 tgccgccgcc gctggaaccg gcgaacgcga tgtcgagtac ggcgacacgc cctttcaatt
    20401 gatctgctcg agggagcttg cggggcgtgc caaaggcgag ttgcatgggg tccccagctt
    20461 ggcacatcgc gccgaaattc acactgtcgc tggcggccgg ccggctagct acgaccgggc
    20521 aaatccaatc gcttcacgga tcgcgcgcac ccgattcgga ccgggcggcg cgaccgagcg
    20581 cgcgtggtac ggtccgctcc gtgtctgaga agcccgtttc cgatccgaag ctcgaaccct
    20641 tccagcgagc gctagctcga cctttatctg gtacccactt cgattggggc acgcgctacg
    20701 aaggcaaagt acgggacaac tacagctgcg ccgggggcaa gcgtttgttg gtcgttaccg
    20761 atcgcatcag cgccttcgat cgagtgctcg ggacgctgcc cttgaagggg cagctgctga
    20821 acttcgtatc caattggtgg ttcgagaaca cccgcgacgt cgcagacaac cacatgcttg
    20881 gcacgcccga tccgaatgtg ctggccgccg tggagtgcga tccgctcccg gtcgagatgg
    20941 tggtgcgggc ctatctgacg ggcacgacgt cgaccagcat ttgggttcac tacgcgcgtg
    21001 gtgagcgaac tttctgtggg caccgcctgc ccgatggctt gaagcagcat cagcgattac
    21061 ccgagctgtt gctcacgccg accaccaaag cgaccaaggg tgggcacgac gtgtccgcgt
    21121 cgcgcgagga aattct
//
LOCUS       DASTKU010000467         9130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_757721, whole genome shotgun sequence.
ACCESSION   DASTKU010000467 DASTKU010000000
VERSION     DASTKU010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9130
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757721"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFQ61_16190"
     CDS             complement(<1..555)
                     /locus_tag="VFQ61_16190"
                     /inference="COORDINATES: protein motif:HMM:NF017730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HET9956048.1"
                     /translation="MATDTLQKLLKSVRLPEEVKAPRPMIAAHMDDVMEDVTPEERFI
                     SSMAALVLNLDSGEAKFDKQNIQALVSSIDEMVQGQLNEILHAPSFQNMEATWSSIAE
                     LVANTNFKAQIDLNLLDVSKEEALEDLELNIADIGGSELFKKVYVAEYDQFGGSPYGA
                     IIGLYEFENKPSDIVWLRGMGKVAS"
     gene            complement(612..1409)
                     /gene="tssB"
                     /locus_tag="VFQ61_16195"
     CDS             complement(612..1409)
                     /gene="tssB"
                     /locus_tag="VFQ61_16195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HET9956049.1"
                     /translation="MAITDEIPRSRITLTYRTTVRGEPEDVTLPFRVLIMGDLSAGTS
                     TDRTQELDKRQIRRLDGKNLNQVMRDMRMSLRFNVKNRVNPKSSADEFEVVLPIDSTK
                     SFQPAEVAKHVPRIRALLLLKKLLLEAQANFDNRKEFRGLLREIAQSSTSLADLVKEL
                     PGGFDTYKLPAAKLKLTVAPDIAKLDKLEVRRATLVPDPLGKAPGGELRKDEEIVPAA
                     QFNQPVRIKPGKIEVRVFAPGKKPWSKTAVVEHDATIDLTVGPLEDA"
     gene            complement(1508..2119)
                     /locus_tag="VFQ61_16200"
     CDS             complement(1508..2119)
                     /locus_tag="VFQ61_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956050.1"
                     /translation="MSGDTNGLDYDCDFRLGFNLDPNRKGTVGYILFWSGCGGLSLSK
                     DIEVWNPFGGTGQTITSGPTIQCVGLIDSFRFTGESDAPIRVVAYVSKDTAANVRAKL
                     ANPITATALQVSWYIVAFDDEKKQWFEAAFIKNGAKAQAAVDVMDGVLQLFISNKPTA
                     VDERLDLKVYKFEFQIVPNEGSSSLLEFATGASQRLVKQWTVD"
     gene            complement(2291..3280)
                     /locus_tag="VFQ61_16205"
     CDS             complement(2291..3280)
                     /locus_tag="VFQ61_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956051.1"
                     /translation="MFNAERSKLASLEARVQSTLAKPRAELAALNAELARRPQLKPTF
                     DGSLRPIQTGLSALQSSLLTPIQSSRQALDQASSAITTTIQQGIAGLDALKSQAVDRV
                     QQLLAQFESAVNTAKSTLESVRTGIKQLADSVLTPVLALKTALKQIETAFKTGVQALN
                     TALSTAMSAVEAIPVSALPKALVATTQSAINTALSTAASALTSASQQAGAQLSSLATS
                     VVKQIDTAKTQALTQLDTLTKALVTQIDAVLPTLQAQLSAARSAIGNAVSQLTAQINA
                     AVQSAKATVEATKSRVSESVATLESTLISQLESGADALFNSLNSVRQQLSSTR"
     gene            complement(3294..5219)
                     /locus_tag="VFQ61_16210"
     CDS             complement(3294..5219)
                     /locus_tag="VFQ61_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956052.1"
                     /translation="MFEELLQATLSLTVGASTFEAKAGSIELLELDARVYGFEARVRF
                     RVSSEESADSLFDPFNSDPVMRATLSLANGRLTLAGEVATAAVFVGYVSERSFVETTS
                     ADLTSSPVIDRAYEVRFADAARVFWSEHRPLAVYANHSFKDVFDANQVEGVTLAYDLP
                     AFDQAYDVLCVGTGSLARASFYDFALWLIDDCTGVIELDASTGSYRIGKDKTDPPSFN
                     QLPGDTIAELRIVHPPRWRHATRVINPFTEAAQHTMDVANSLAGSGVRRDVVSYTPVV
                     KEMEQRATLERDRLRQRKPHARVTFARLPELLPIPNSGLEFGEGSSPSGYCAGKSFRV
                     IEFELRARLPDGAGGTDYGDANQRFAIDLSIELELASDPVPILPEYMLPTYPVMAEGK
                     VLSASGAESSRTWHALAGQSDSILRYRVQVPLWNQTVVVPFAPGGESGHFFFPAYKNQ
                     RVLIAFELTGGRIVGFLDWASKLAIETQGNQLVMGKTETSSTIFKHVYTDESPVLTLV
                     RNEAGDSQTIELSDGRFFLEVKAEESQDTGVQTYDLTPQAEVAKDAASSQARSAIADL
                     TGSYQSSMASASSELDAASDELSQGVGAASASLAQKTAEVEAQLSSQTSELEALADDL
                     DAQVSQAKAELTALIED"
     gene            5449..5796
                     /locus_tag="VFQ61_16215"
     CDS             5449..5796
                     /locus_tag="VFQ61_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956053.1"
                     /translation="MATWKTAEQLSELYFIGADRLLAYSRRGNLPRTCTEQGVYLFDE
                     EVAACLFRRRSAAGVEAPVSWGVLGEARLGAASPAQTSTEEPCAASARVVRARALRLG
                     RPVPSELARRTGS"
     gene            complement(5847..6230)
                     /locus_tag="VFQ61_16220"
     CDS             complement(5847..6230)
                     /locus_tag="VFQ61_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956054.1"
                     /translation="MTRFDLARLRHNFDRCHPPEVPALPVRFARVREPRAVGPEARLL
                     LAAIRRIVERDFSAQLTSLRPFLDETSALLDRLERAAAPTEGQPSPSVDPTPAENASE
                     LRQQLRVALQDVEDLLEVYSGMGRR"
     gene            complement(6253..7011)
                     /locus_tag="VFQ61_16225"
     CDS             complement(6253..7011)
                     /locus_tag="VFQ61_16225"
                     /inference="COORDINATES: protein motif:HMM:NF021377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HET9956055.1"
                     /translation="MDTKTALWFAIDDTFAELYELCLEARAAELVVQQRQAEELVVRS
                     LSAGRTAPQTALPTEEGRYARAVDLAREASFRERHAAGADIVMMRASIRRRLGELRAR
                     LAEVLAEHEVYYVLFPIVVYADELVATATRGAANRWEPMQSEFYEIDNGGELFYVVCE
                     ERLRQDETHPLVFEAFYFCLADGFTGMYPSGSKKIEDTKARLLERIAKPELRFPELKR
                     ESRRAELVAFPWWVYAVAASTLLVVYTALSWSAG"
     gene            complement(7027..8676)
                     /locus_tag="VFQ61_16230"
     CDS             complement(7027..8676)
                     /locus_tag="VFQ61_16230"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HET9956056.1"
                     /translation="MSQDPIERAFLTELEALEKFRIAYTAQYPSVPLSHDDPDVRRMI
                     EALAFFTARTRQAALRGLDESVQRVFRQHFPALLGPCPAMAMLRAVPSSTFTELTELP
                     RGTEVLLREAPDEYGAGGKVFRFKTLADLTILPLSVRAVELSKRRGQGFRLLIRLVMS
                     NPTNHELASFRLHVNHLDDLRSSITVLHELRTHLRGASVLYDPKAAEDAMGAPCQVSF
                     GSLESEEELPDPFESPLQRARLRLRFPRQELFLNVAGLRPPRNWQHLTLCLDLSDEWP
                     RKLRLTADGFELHAVPMINVTRSMANPVLCDGTKDRYPIHHPDDSGEYAPLWVIGAYR
                     PTKQGFVPIAPGVMGSGGDRYEVVSAGRGNERRAWVMLNLQGAFQKPEPISIDGFWYQ
                     PGLRAAEFSELSVGLHAQFVEGVSWQCSGPLVPDAESALDGDRDAQLQLVSLKTVRFL
                     SYDQLMLLVRACGAADEPLFAPVVAGLRDVQVSDKPHGTRGRSLKYTYELRFESLEKS
                     DRARLSMFCAWLLRLLTTWSVEEVVEIVAVVRNWEQPLQFR"
     gene            complement(8711..>9130)
                     /locus_tag="VFQ61_16235"
     CDS             complement(8711..>9130)
                     /locus_tag="VFQ61_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956057.1"
                     /translation="EIKCKSFSVDAETVSVKSSKTTSHEATETFSIQSTKDMTLTSSA
                     KLSLSSTQDMSLSSSGALAQSATGDASLKGNNTNLEANLKLSAKGGVDLAASATKMAV
                     TADAKIDITSPMTTVGDTVTTVKGQMVKVEGSLVKLG"
BASE COUNT         1711 a         2854 c         2739 g         1826 t
ORIGIN      
        1 ggaagcgacc ttccccatgc cgcgtagcca gacgatatcc gacggcttgt tctcgaactc
       61 gtagagcccg atgatcgccc cgtaagggct gccaccgaac tgatcgtact cggccacgta
      121 gaccttcttg aacagctcgg atccgccgat gtcggcaatg ttgagctcga gatcctcgag
      181 cgcctcctcc ttgctgacgt cgagcagatt gaggtcgatc tgcgccttga agttggtatt
      241 ggcgacgagc tccgcgatgg aggaccaggt cgcctccatg ttctgaaagg acggggcgtg
      301 caggatctcg ttcagctgac cttgcaccat ctcgtcgatc gacgagacca acgcttgaat
      361 gttctgcttg tcgaacttgg cttcgcccga gtccaaattg aggaccagtg cggccatgct
      421 cgagatgaag cgctcctccg gcgtgacgtc ctccatcacg tcgtccatgt gagccgcgat
      481 catcggacgc ggggccttga cttcttcggg aagcctcacg ctcttcaaca acttttgcag
      541 ggtatccgtt gccatgccga gaaaactcct attcagctat tccgttgcta cggctcgcgt
      601 tcgcgcgggg gtcacgcgtc ctcgagcggg ccgaccgtga gatcgatagt tgcgtcgtgc
      661 tctacgaccg ccgtcttgga ccaaggcttc ttgccgggtg cgaagacacg gacctcgatc
      721 ttgcctggct tgatccgcac gggctggttg aactgcgcgg cggggacaat ctcttcgtcc
      781 ttgcgcagct ctccgcccgg cgctttgccg agcgggtcgg gaacgagggt ggcgcgacgc
      841 acctccaatt tgtcgagctt cgcgatatcg ggcgcgaccg taagcttcaa cttcgccgcc
      901 ggtagcttgt aggtatcgaa gccaccgggt agctccttga ccaaatcggc aagggaagtt
      961 gaactctgcg caatctctcg tagcagcccg cggaactcct tgcgattgtc gaagttcgcc
     1021 tgcgcctcca gaagcagctt cttgagcagc agcagcgcgc ggatgcgcgg cacgtgcttg
     1081 gccacctccg cgggctgaaa ggatttggtc gagtcgatcg gcaacaccac ctcgaactcg
     1141 tcggcactcg atttggggtt cacgcgattt ttgacattga acctcaagga catccgcatg
     1201 tcgcgcatga cttggttcag gttcttgccg tcgaggcgcc gaatctgcct tttatcgagc
     1261 tcttgggtgc ggtcggtgga cgtgcccgcc gacaggtcgc ccatgataag gactctgaaa
     1321 ggcagcgtta cgtcctcggg ctcgccgcgt acggtggttc tgtaggtaag cgtgattcta
     1381 gatcgcggaa tctcgtccgt tatcgccatt ggttctccgt tgtggaagaa gatcgaaatc
     1441 gttcgttagg aactgagagc tgaccgttac gcagcaacgc tccggccagt gacgcagaat
     1501 caagcgttca gtcgacggtc cattgcttga ccaagcgctg gctcgccccg gtggcaaact
     1561 cgagcaagct gctgctgcct tcattgggca caatctggaa ctcgaacttg tagaccttca
     1621 ggtcgaggcg ttcatcgacc gcggtcggct tgttgctgat gaagagctgc agaactccgt
     1681 ccatcacatc cacggccgct tgcgctttgg caccgttctt gatgaaggcc gcctcgaacc
     1741 actgcttttt ctcgtcgtcg aaagcgacga tgtaccagct cacctgcaaa gccgtggccg
     1801 tgatcgggtt ggccaacttt gcgcgcacgt tcgcggccgt atctttgctg acatacgcga
     1861 cgactcggat cggcgcgtcg ctctcaccgg taaatctgaa gctgtcgatc aagccgacgc
     1921 actgaatggt gggccccgaa gtgatggttt gccccgtccc gccgaacggg ttccaaacct
     1981 cgatgtcttt gctcaggctc agcccaccgc aaccgctcca aaataatatg tacccgacag
     2041 ttcccttgcg attcggatcc aggttgaagc cgagccgaaa gtcgcagtca tagtcgagac
     2101 cgttcgtgtc gcctgacatg ggagccctct cgccgggcga gccggcgcgc ctcgctgact
     2161 cgccgtgccg ggttttcacc cgcggcacgt ccactcaaag tgacatgatg atagccatgc
     2221 aggcaattaa agtgtcggaa cggcgggcac cgtcgaggtg gcgatctccg cacgccaccc
     2281 cacctgtcgc tcatcgggta gacgacagct gctgtcgcac ggagttcaga ctattgaata
     2341 gcgcgtcggc tccactctcg agttgtgaaa tcaacgtact ctcgagtgtg gcaacggatt
     2401 ccgacactcg gctcttggtg gcttcgacgg tggccttcgc ggattgaacg gccgcgttga
     2461 tttgcgccgt caattggctg acggcattgc cgatcgcgct acgcgcggca gagagctgag
     2521 cctgaagagt cggcagtacc gcgtcgatct gagtcaccag cgccttcgtc aacgtgtcga
     2581 gctgggtgag ggcctgcgtt ttcgcggtat cgatctgctt cacgacgctg gtcgcgagcg
     2641 agctcagctg cgcgcccgct tgttggctcg cgctggtcag cgcggaagcg gccgtggaga
     2701 gagcggtgtt gatcgcagac tgggtggtgg cgacgagcgc cttgggcaat gcggaaacgg
     2761 gaatggcttc gaccgcgctc atggcggtcg aaagggcggt gttcagggct tgaacgccgg
     2821 tcttgaaggc ggtctcgatt tgcttgagtg ccgttttgag cgcgagcacg ggagtcagca
     2881 cgctgtctgc cagctgtttg atgccagtcc gaactgattc gagtgtgctc ttggccgtat
     2941 tcacggccga ttcgaactgt gcgagcagct gttgaacccg atcgacggct tggctcttca
     3001 gcgcgtccaa accggcaatc ccttgctgga tcgtggtcgt gatggcggaa ctcgcctggt
     3061 cgagcgcctg acgcgagctc tgaatcggcg tcaggagtga gctttgcagg gcgctgagcc
     3121 cagtttggat cggacgcagg ctgccatcga aagtgggctt gagttgcggc ctccgagcca
     3181 gctccgcgtt cagcgcggcg agctcggctc gcggtttggc caaagtggac tgaacccgtg
     3241 cctcgaggct cgcgagcttc gagcgttccg cattgaacat tggaaaagcc ctttcagtct
     3301 tctattaacg cggtgagctc ggccttggcc tgcgatacct gtgcatcgag gtcgtccgcc
     3361 aaggcctcca attccgaggt ttggctcgac agctgcgcct cgacctcggc agtcttctgg
     3421 gcgagcgagg cgctcgccgc gccgacgccc tggctgagct cgtcagatgc cgcatccaac
     3481 tcactggatg ccgaagccat ggaggattga tagctgcccg tcaaatccgc aatggcggat
     3541 cgggcttgag acgaagccgc gtccttcgca acctcggctt gcggcgtcag atcgtaggtc
     3601 tggacaccgg tgtcttggct ctcctccgct ttgacctcca gaaagaagcg cccatccgag
     3661 agttcgatcg tctggctgtc cccggcttcg ttgcgtacga gcgtgagcac gggactttcg
     3721 tccgtataaa cgtgcttgaa gatagtactg ctcgtctccg tcttacccat caccagctga
     3781 ttgccctgcg tctcgatcgc gagtttggac gcccagtcca ggaagccaac gatgcggccc
     3841 cccgtcagtt cgaaggcgat gagcacgcgc tggttcttgt aggcggggaa gaagaagtgg
     3901 ccggactctc cccccggcgc gaacggaacg accacggttt gattccaaag cgggacttga
     3961 actcgataac gtaagatcga gtcgctctga ccggccagtg cgtgccaggt ccggctgctt
     4021 tcggcgccgc tcgcgctcag caccttccct tccgccatga cggggtaagt cggaagcatg
     4081 tattccggca gtatcggaac cggatccgat gccagctcga gctcgatgct cagatcgatg
     4141 gcgaagcgtt ggttggcatc tccgtaatcc gtcccaccag cgccgtcggg caagcgagct
     4201 cgcaactcga actcgatcac ccggaagctc ttcccggcgc aatacccgct cgggctcgag
     4261 ccctcgccga attcgagacc ggagtttgga atcggtagca gctcgggtag tcgcgcgaag
     4321 gtcacgcgtg catgcggttt gcgctgccga aggcgatccc gctcgagggt tgcccgttgt
     4381 tccatttcct tgacgacggg tgtgtaactc accacatcgc gccgcacgcc gcttcccgcc
     4441 aggctgttgg cgacgtccat cgtgtgctgg gcggcttccg taaaagggtt gatcacccgg
     4501 gtcgcgtgcc gccaccgagg tggatggacg atacgtagct ccgcgatggt gtcgcccggc
     4561 agctgattga aactcggtgg atcggtttta tcttttccga tgcgatagct gccggtggaa
     4621 gcatcgagtt cgatcactcc cgtgcagtcg tcgatcaacc acagggcaaa gtcatagaag
     4681 gatgcgcgcg ccaagctccc ggtccccacg cacagcacat cgtaggcctg atcgaacgcc
     4741 ggcagatcgt aagcgagggt cacgccctcg acttggttcg cgtcgaagac gtctttgaag
     4801 ctgtgattgg catacaccgc cagggggcgg tgctcgctcc agaacacacg cgccgcgtcg
     4861 gcgaaccgca cctcgtaggc tcggtcgatc actggggacg aggtgagatc ggcactggtc
     4921 gtctcgacaa agctgcgctc gctaacatag cccacgaata cggcggccgt cgctacctcg
     4981 cccgccaacg tgagccgccc attggccagg ctgagcgtag cccgcatcac gggatccgaa
     5041 ttgaagggat cgaacagcga atccgcgctc tcttcactgg agacgcggaa acgcacccgc
     5101 gcctcgaacc cgtagacgcg ggcatcgagc tccaagagct caatcgatcc tgctttcgcc
     5161 tcgaaagtac tcgcgccgac cgtgagcgag agcgtcgctt ggaggagttc ttcgaacata
     5221 cgagtcctgc gccccgagcc aaccgcgcaa gctaggcggc tgcggcggga agagccaagt
     5281 tttacggctc gattctacgc gagtccgtct cgagtcgaca cagcgtgcgt tctctagcgt
     5341 gagtgctgca ccgtggtcgc accgtgttcg tcgcgagacg ggggcctttt catgagggct
     5401 cgccacaaac tggtccggaa tccgaccgca tgttagtcgc ctcagtgcat ggcgacctgg
     5461 aagaccgctg agcaactttc cgaactgtac ttcatcggcg cagatcgttt gcttgcttac
     5521 agccgtcgcg ggaatctacc caggacgtgc acggaacagg gcgtgtacct cttcgacgag
     5581 gaggtggcgg cgtgcctgtt ccggcgccgc tccgccgcag gagtcgaagc gcccgtcagc
     5641 tggggcgtac tcggcgaagc gcggctcggt gccgcatcgc ccgcgcagac ctccaccgag
     5701 gaaccctgtg ccgcgagcgc ccgcgtggta cgcgcacgtg cgctgcgact cggccgtccc
     5761 gtcccaagtg agcttgcccg gcgcactggc tcttgatgcc gtcggccgag gctcagtatc
     5821 gcaatagccg gattggctac ccgtcctcag cggcgtccca ttccggagta cacctcgagc
     5881 aagtcttcaa cgtcctgcag cgcgacccga agctgttgac ggagctcact cgcgttctct
     5941 gccggcgtcg gatccacgga gggagagggt tggccctcgg tcggggcagc agctcgctcc
     6001 agccggtcta gcagcgctga agtctcatcc agaaacggtc gcaaactcgt gagttgggcc
     6061 gagaagtctc gctccacgat gcgacgaatc gcggccagca gcagcctcgc ctccggaccc
     6121 accgcgcgtg gctcccgaac tcgcgcgaaa cgaaccggca aggctggcac ctctggtggg
     6181 tggcagcgat cgaagttgtg ccgcaaccgg gctaaatcga agcgagtcac gtgcgtagct
     6241 ccggggccgc catcaacccg ccgaccacga caatgcggta taaaccacca acagggtgga
     6301 ggcggctact gcatagaccc accacggaaa ggcgaccagc tcggcgcgcc gcgactcccg
     6361 cttgagctcg ggaaaccgga gctcgggctt ggcaatacgc tccagtagac gggccttggt
     6421 atcttcgatc ttcttcgaac cagacgggta catgcccgtg aatccgtccg ctagacagaa
     6481 gtagaacgcc tcgaatacca gggggtgagt ttcgtcttgc cggaggcgtt cctcacacac
     6541 cacataaaac agctcaccgc cgttgtcgat ttcgtagaac tcactctgca tcggttccca
     6601 acgattcgcc gccccgcgcg tcgctgtcgc cacgagttca tccgcgtaga cgacgatcgg
     6661 gaaaagcacg taatacacct catgctcggc caaaacttct gccaagcgcg cacgcagctc
     6721 gcccaatcgg cgtcggatac tggctcgcat catcacgata tccgcccccg ccgcgtgccg
     6781 ctcgcgaaag gacgcttccc gagcgagatc caccgcgcgt gcgtagcgtc cctcctcggt
     6841 aggcagcgct gtctgcgggg ccgtgcgacc agcgctgagc gagcgcacca cgagctcttc
     6901 ggcttgcctc tgttgtacca ccagctccgc cgctcgagct tccagacaca gctcgtaaag
     6961 ctccgcgaag gtgtcatcga tggcaaacca taatgccgtc ttcgtatcca tggtgcgacc
     7021 tcagcactac ctgaactgaa ggggttgttc ccaatttcga acgaccgcga caatctcgac
     7081 gacctcctcg acgctccagg tcgtgagcag acgcaacaac catgcacaga acatactcaa
     7141 acgcgcgcgg tcggacttct ccagcgactc gaagcgtagc tcgtacgtgt atttcaaact
     7201 gcgcccccgg gtgccgtgtg gtttgtcgct gacctgcaca tcacgaagcc cggccacgac
     7261 tggagcaaac aaaggttcgt cggcggcgcc acaggcccgc accaagagca tgagttgatc
     7321 gtagctcaag aagcgcaccg tcttcagcga cactagttgg agctgagcgt cacggtcccc
     7381 gtcgagggcg ctctccgcat cgggcaccag aggaccagaa cattgccagc tgacgccttc
     7441 cacgaactga gcgtgcaacc cgacggaaag ctcagaaaac tctgcggccc gaagccctgg
     7501 ctggtaccaa aacccgtcga tcgagatcgg ttcgggcttt tgaaaagccc cctgcagatt
     7561 cagcatcacc caggcccgtc gctcattccc tcggccggca ctgaccacct cgtaacgatc
     7621 tccaccactg cccatgaccc cgggcgcgat gggcacgaac ccttgcttgg tgggtcgata
     7681 agcgccgatt acccaaagtg gagcgtactc acccgagtca tcggggtggt gaatcgggta
     7741 gcggtctttg gtcccatcgc acagcacggg attggccatg ctccgtgtta cattgatcat
     7801 cggcaccgcg tgcagctcga agccatccgc ggtaagacgc aatttgcgcg gccactcgtc
     7861 ggaaaggtcc aagcacagcg tcagatgttg ccaattgcgc ggtggccgca ggcccgccac
     7921 attcaggaac aactcctgtc gcgggaacct caggcgcaac cgcgcgcgtt gaagtggact
     7981 ctcgaatgga tctggcagct cttcctctga ctccagcgat ccaaagctca cttggcaagg
     8041 cgcgcccatc gcatcctccg ccgccttggg gtcatagagc acgctcgcgc cgcgcaggtg
     8101 tgttctcagt tcatgaagga cggtgatgga tgagcggagg tcgtccaggt gattgacgtg
     8161 caggcgaaaa ctagcaagct catgattcgt tggattgctc atgaccaatc gaatcaacaa
     8221 tcgaaagcct tgacctcttc ttttggagag ttcgaccgcc cgcacggaca gcggcaaaat
     8281 cgtaaggtcc gcgagcgtct tgaaccggaa tacctttccc cccgcgccgt actcgtcggg
     8341 tgcttcgcgc agcaaaacct ccgtgccccg cgggagctcg gtcagctcgg tgaaggtgga
     8401 gctcggaacc gcgcgcaaca tggccatcgc gggacatggc ccgagcaatg cgggaaagtg
     8461 ctgacggaag acgcgctgca cactttcgtc caagccgcgc aaagctgctt gtcgggttcg
     8521 cgccgtaaag aaggccaggg cctcgatcat ccgcctcacg tcggggtcat cgtgagagag
     8581 cggaaccgac ggatactgcg ccgtgtacgc gatccggaac ttctcgaggg cctcgagttc
     8641 ggttagaaat gctcgctcga tcgggtcttg tgacatccct cgacgcctca cctcgcagag
     8701 aactacccca ctagcccaac ttcaccagag agccttcgac tttcaccatc tgccctttga
     8761 cggtagtaac ggtatcgccg actgttgtca taggagaggt gatgtcgatc ttggcgtccg
     8821 ccgtgacggc catcttcgtc gcactggccg cgagatcgac gccacctttg gccgagagct
     8881 tcaggttcgc ttcgaggttc gtgttattgc ctttcagcga tgcgtcacca gtcgcgctct
     8941 gggccagagc accgctcgaa gacagtgaca tgtcctgggt gctcgacagg ctcaacttcg
     9001 cgctcgaggt gagggtcatg tccttcgtgc tctggatcga gaaggtttcc gtagcctcat
     9061 gactcgtggt cttgctggac ttgaccgaga ccgtctcggc gtcgacgctg aaggacttgc
     9121 acttgatctc
//
LOCUS       DASTKU010000468         8199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_757739, whole genome shotgun sequence.
ACCESSION   DASTKU010000468 DASTKU010000000
VERSION     DASTKU010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8199
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757739"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            733..963
                     /locus_tag="VFQ61_16240"
     CDS             733..963
                     /locus_tag="VFQ61_16240"
                     /inference="COORDINATES: protein motif:HMM:NF014059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin family protein"
                     /protein_id="HET9956058.1"
                     /translation="MRSIEARYEQGILKLTQPLPLTPGERVRVIVVRQPDPKRWDLDK
                     LRHADATEDLALAEAGLSAWDQALDSDQEGAG"
     gene            960..1313
                     /locus_tag="VFQ61_16245"
     CDS             960..1313
                     /locus_tag="VFQ61_16245"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HET9956059.1"
                     /translation="MKRGELWWANLGEHRPLEQTGRRPVIIWQSDALNSVLQSVLVVP
                     LTTNLDRAHLAGTAMIEASELGPPQDSVALAFQMRAIPKRALEAKMRTLTDVETAELE
                     MATDEALGRLDPEAT"
     gene            1653..2822
                     /locus_tag="VFQ61_16250"
     CDS             1653..2822
                     /locus_tag="VFQ61_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS domain-containing protein"
                     /protein_id="HET9956060.1"
                     /translation="MAIRTALWKVGQDPTPLQRSSLPKEQLLEDMIVRAPALLSQEWM
                     LIGRQEDTGFGGRIDLLAIAPDASLVLIELKRDRTPREVVAQAIDYASWVEGLKAEDV
                     REIYERFAPGRLLAEDFQSRFGGILDEEALNQNHQIVIVAGSLDASSERIIGYLSGRG
                     ISINVLCFEVFALNGELLLSRAWLLDPIESQINAAQPERSREPWNGEYYCSFGHDVER
                     NWEDARELGFVSAGGGSWYTKTLQLLSPGSRIWVKVPGIGFVGVGQVAGSAQPISEFR
                     IKGIPIRQLAKRSRFGEGVDDDPELCEYFVPVEWVQTTSLDDAVKEIGLFGNQNTVCK
                     PTTPKWRSTVERLKQHFARANPDPSTPVNGDVHSADALDGTRVKGTQKVAAQLGH"
     gene            3414..3641
                     /locus_tag="VFQ61_16255"
     CDS             3414..3641
                     /locus_tag="VFQ61_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956061.1"
                     /translation="MGQPAAKLSVSVPGELAEAVRKRVGAKGLSGFVARALAHELERE
                     GVGALLMELEEQLGPPSKANLARARKAWPKR"
     gene            3626..4042
                     /locus_tag="VFQ61_16260"
     CDS             3626..4042
                     /locus_tag="VFQ61_16260"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET9956062.1"
                     /translation="MAEALILDSEAVNALARPTERRVLAERAGAILRIAHQKRALIRV
                     PAPVLAEVCRGVRHDSAINHLLNNPGVRVAELDRTAAQQAGQMLARLKLSSEHAIDAF
                     VVATALQFDTAVIATGDPDDIRRLAAPFSRIGVFAL"
     gene            4228..5280
                     /locus_tag="VFQ61_16265"
     CDS             4228..5280
                     /locus_tag="VFQ61_16265"
                     /inference="COORDINATES: protein motif:HMM:NF014048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS domain-containing protein"
                     /protein_id="HET9956063.1"
                     /translation="MSNVALWQVGAGSLERVPEVALDLESTLESWIETTPALIQSDLV
                     IVGRQLKTEAGRIDLLGLDAQGRWLVIEVKRAVLSRDAVAQVLDYASLLVSLDAEALA
                     ELTNAYLEPRGKSIEGLLQERDAMESLSVQPREVLLVIVGTGRAAGLDRVVQYLSDRY
                     GVPISVVTLDVFELPAGPRLISREVLEAEDLADSERQRTRISPDDVIALARGAGLGEG
                     FEAIYRAAIELGLYPRTWKTSIMYASPKKRTNMLFTVWARPTDGKLRLSVWHQGFHDF
                     YGFSVEQVAQYLGPEGDRFFTVADAHEFAEKLRALFAALAPQAQEGQAAADKPRLASK
                     TETGGSGTESDGTLPN"
     gene            5526..6068
                     /locus_tag="VFQ61_16270"
     CDS             5526..6068
                     /locus_tag="VFQ61_16270"
                     /inference="COORDINATES: protein motif:HMM:NF045464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956064.1"
                     /translation="MQRQSGRTDLSVAAALGPARLLGGRGAAIREPLSCGSGGLGMDL
                     VVWFLRRTPDLGTLVLGPPQLCFGIDVNLPNADQAIIPLEKVRDYLLSAANPRARGKA
                     AFFQTLGFDPVQWEVLRDALLEVARTGDASPGQRSEFGTKYEIRATICGPTGRRAVIR
                     TVWIVNAGEDRPRFITGYPD"
     gene            6068..6301
                     /locus_tag="VFQ61_16275"
     CDS             6068..6301
                     /locus_tag="VFQ61_16275"
                     /inference="COORDINATES: protein motif:HMM:NF027602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HET9956065.1"
                     /translation="MATELHQEVILARDVPQHGLRVGDIGTVVHIYPKGGLEVEFFTA
                     SGKTRAVVTLRDADVRTATDNDVVAVRKLDATG"
     gene            6769..7122
                     /gene="tnpB"
                     /locus_tag="VFQ61_16280"
     CDS             6769..7122
                     /gene="tnpB"
                     /locus_tag="VFQ61_16280"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET9956066.1"
                     /translation="MIPSGVEVFIGLVPIDLRWGFERLAGLAQEHIGRSARSGALFVF
                     VGKRKTALKILFFDGSGMCLFYKRLDRRTFRVPEVPSPGILSIEMGERELDDLLDGID
                     VEPESTPPRARKRLH"
     gene            7169..8116
                     /locus_tag="VFQ61_16285"
     CDS             7169..8116
                     /locus_tag="VFQ61_16285"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9956067.1"
                     /translation="MSTNLLVRGSDVDVLDPRFAAHPELYALLERERTQWARERASLA
                     EHITLLEQERDRLRASHERLRQELELFKRRLFIAKAERIDTRQLELEYAQKLRELDTL
                     AGTLGLAKEPAQAEDPTPASDGKRRGKRKGNRGTGRRDLRLLSLEEERIEITDPHLEA
                     LVAEGKVVRHGFEETYKLGHKRGGRRRIVIARVRYKTVDANGDTDVITTAMPEQMLPS
                     ALAAPSLVAHVIMENVGKGMPLFRIEDGAARDGIPLDRGSLSRWKRAVGERLASTVVE
                     AMWKHALATAFCISTDATGICIGCRSFRTVVNSTVYATF"
BASE COUNT         1529 a         2455 c         2672 g         1543 t
ORIGIN      
        1 tgggcgtttg cggcctaacg ccggtgatag cgggattaga gggagccaga taacgcgatg
       61 ccgggaagtg cggattgggg gaggcggcgt gcagggattg gggccgtggg gttcgcgtgg
      121 attgcggagg ggcgtgcggc aaaataggag ttatgccgac aaccaatgcc gacgcgcgat
      181 cgcggcgatg ggccgcctag accgacgtgc tccagagtac gccggccgtg gtcacgcaag
      241 accgcgtaca cggcaacttc gcctgcgtcg gttcccatgc gaagcggtcc gcggcacagg
      301 tcgtcggacg ggctcgcggc acagtcctcg aggttcagtt cgtgcgttgc cgggcgatcc
      361 tgcctcgctg ccttgttggc tgcgcccaag gcagcggttc tcgtcatcaa cgggcgttgg
      421 acgcacttcc ggcaagcggc ccatgatgca tctggatcct ctacacacga cgttcaggta
      481 tgggggcgcg cggtgcggtg cctgttggcc tgtaggactt tgggacgagc ttgcctcgct
      541 gccttgttgg ctgcgcccaa ggcagcgggt tgccgcagcc cgtgcgtgcg ctcgagaatg
      601 agcgctgcca ggtgccggac tccgggcgct tcgtgcgacc ggagaaccag cgctgaaacg
      661 cttgatttct gctaccgatg acggtctccg tcgcgtatgg gcacatcggg tccggtgtgc
      721 tacaattctc gaatgcgttc gatcgaggcc aggtacgagc agggcatcct gaagctaacc
      781 caaccgctgc cactgacgcc tggtgaacgc gtgcgcgtca tcgtggtgcg gcagcccgat
      841 ccgaagcgtt gggaccttga caagctgagg cacgcggatg cgaccgagga tttggcgctg
      901 gccgaagccg gtctgtccgc ctgggaccag gcgctcgatt ccgatcagga gggcgctggt
      961 tgaagcgcgg cgagctctgg tgggcgaatc ttggtgaaca ccgcccgctc gaacagacgg
     1021 gtagacgccc cgtcatcatc tggcaaagcg acgcattgaa cagcgtgctg caatcggtgc
     1081 tggtcgttcc gctgacgacc aatctggatc gcgcccacct ggctgggacg gcaatgatcg
     1141 aagcgtcgga actcggaccg cctcaggact ccgtggccct ggcgtttcag atgagagcca
     1201 tacccaaacg ggcgctcgag gcgaagatgc gcacgctaac cgacgtcgag acggccgagc
     1261 ttgagatggc tacggacgag gccctcgggc ggctggaccc cgaggcaacg tgagtgcggc
     1321 ataactcggc cgttgcagct gacgtggccc tcagtcgctg cgctccttcg ggaccacgca
     1381 gctgaacgcc agtcgttggg cggacgagtt cggttgtccc ctcacgccgg cggctcgctg
     1441 cggcgcttct ccaatgatgc tccggcatgc cgcgacattc gagcgcggcg gcgtcggact
     1501 tgccgatcgc catgggggac agatcgtcaa cggaggcacg cgcagacatg acgtacgccg
     1561 cgtggccgcg tcggccccaa ggagccctct cgtcttggaa gcaagtgctc cacacgtgca
     1621 gatgggccgc cgacgtgtat gatcaggcta caatggcgat ccgaacagcg ctctggaagg
     1681 tcggacaaga ccctacacct cttcagcgtt cttctttgcc caaggagcag cttcttgaag
     1741 acatgatcgt tcgagcgccc gctctgcttt cccaggagtg gatgctcatc gggcgccagg
     1801 aagacaccgg cttcggcggg cggattgacc tattggcgat cgcaccggac gcgtcgttgg
     1861 tgctcatcga gctcaaacgc gaccgaacgc ctcgcgaagt tgtggcgcag gcgatcgatt
     1921 acgccagctg ggtggaaggg ctgaaggcag aggacgttcg cgagatctac gaacggtttg
     1981 cgccgggacg tcttctcgcc gaggactttc aatcgcggtt cggtggcatt ctcgacgaag
     2041 aggcgctcaa tcagaaccat cagatcgtga tcgttgccgg ctctcttgac gcgagttcgg
     2101 aacgcatcat cggatacctg agcggtcgcg gcatcagcat caacgtcctg tgctttgaag
     2161 tgtttgcgct caacggggaa ctgctcctca gccgagcatg gttgctcgat ccgatcgaat
     2221 cacagatcaa tgctgcgcag ccggagcgtt ctagggagcc atggaacggg gagtactatt
     2281 gctcgttcgg gcacgacgtc gaacggaact gggaggatgc tcgcgaactg ggcttcgtca
     2341 gcgctggagg cggctcgtgg tataccaaga ctctccagtt gctcagccct ggaagccgca
     2401 tttgggtcaa ggttcccggt attgggttcg tcggcgtcgg gcaggttgcc ggttcagcgc
     2461 agcccatttc cgagttcaga atcaaaggga ttccgatacg ccagctggcc aagcgcagca
     2521 gattcggcga gggggtcgac gatgatccgg agttgtgtga gtactttgta cctgtcgagt
     2581 gggttcagac gacctcgttg gacgacgccg taaaggagat cggcctcttc ggcaatcaga
     2641 acaccgtttg caagccgacc accccaaagt ggcgttcgac ggtggagcgt ctcaagcagc
     2701 actttgcacg tgccaacccc gatcccagta cgccagtgaa cggcgatgtc cacagtgctg
     2761 atgctttgga cggaacccgc gtcaaaggca cgcagaaggt cgccgcccaa ctcggacatt
     2821 gaagctgacg tggccctcag tcgctgcgct ccttcgggtc cacgcagctt aatgccagtc
     2881 gttagctaga cttccgaatc cgctggcgag ctcgacatcg cgccatggag attcagacct
     2941 gggccgctat cgccgaagtg cgcgtcgttt tatgacgccc ccagcaggta cgacggtgcc
     3001 ggtgaccatc aaacttgatt gcccggtcgt cgatgcggag cccgcttggc tgcgccagta
     3061 tcggatcagg cgcttggatt cgagcccatt ccgggtaaac caccgtgaac gccacgcaag
     3121 cactcgttct agccgtgcac acgcgctgcc ttcatcagac agcgcgaccg aaggcccatt
     3181 cgatgccggc gacggcgctg gcgtgggatc atcgcctact cgaccggaat cgaagtcgct
     3241 ggcgacgtct tgctgtgcag cgcccggact gtgaaaccga ttccgctggt ccgtcgtgcc
     3301 acgcagaggc tgctgccgcg cagggcactc cggatttccg ctgtctgcca gtcgagcgta
     3361 tcggaccggt cttacagctt gcacatcgat tcctaccctg gtacgaattg gcgatgggtc
     3421 aaccagcagc gaagctgtcc gtcagcgttc ccggggagtt ggcggaggcg gtgcgcaagc
     3481 gtgtaggtgc caaagggctt tccggcttcg tcgcgcgagc tctggcgcac gaactcgaac
     3541 gggaaggtgt tggcgcgctt ctcatggagc tcgaggaaca gcttggaccg ccgtcgaagg
     3601 ccaacttggc tcgcgcgcgc aaagcatggc cgaagcgctg atcctcgatt ctgaggcggt
     3661 caacgctctg gcgcggccga cggagaggcg ggtgctcgct gaacgcgctg gtgctatctt
     3721 gcggattgct caccaaaaac gtgcactcat tcgggtaccg gcgccggtgc tggcagaagt
     3781 ctgccgtggt gttcgacatg actcggccat aaatcatctg ctgaacaatc ccggtgttcg
     3841 cgttgctgag ctggaccgca cggctgcaca gcaagccggt cagatgctgg cccggctgaa
     3901 gctctcatcg gagcatgcaa ttgatgcgtt cgtggtcgcc actgcgctcc agttcgacac
     3961 cgcagtcatt gccaccggtg atccagacga catccgtcgc ttggcagcac ctttttcacg
     4021 aattggcgtg tttgcgcttt gaacgaatcg ccgtgaaacg cgctagctaa ctcggacatt
     4081 gaagctgacg tggccctcag tcgctgcgct ccttcgggtc cacgcagctt aacgccagtc
     4141 gttcggcaga cgaagcgcga gcttgccagc gtccctgaac gacgcacagc gcttaagtgc
     4201 cttacgaaga gttgcgtaag cctcgctatg agcaacgtcg cactgtggca ggtaggagct
     4261 ggctctctgg agcgcgtgcc ggaggtggcc ctggatctcg agagtactct cgaaagctgg
     4321 atcgagacga ctccagcgct gatccagtcg gacctcgtca tcgtcggccg gcagctcaag
     4381 accgaagcag gtcggatcga tcttctcggt ctcgacgcgc agggacggtg gctcgtcatc
     4441 gaagtcaagc gagccgtgct gagtcgagac gcggtcgcac aggttctcga ttacgcctcg
     4501 ctgcttgtgt cactcgacgc tgaggcgctc gccgaattga cgaacgcgta ccttgagccg
     4561 cgcggcaagt ccatcgaggg cctacttcag gagcgcgacg cgatggaatc actgtctgtg
     4621 cagccacgtg aggtgttgct ggtgatcgtc ggaacgggtc gcgccgctgg tctggatcga
     4681 gtggtgcagt atctctctga ccgatatggt gtccccattt ctgtggttac gctggatgtc
     4741 ttcgaattgc ccgccggccc gcgcttgata tcgcgtgagg tccttgaagc tgaggacctg
     4801 gcggattcgg agcgacagcg gacccggata tcacccgatg atgtaattgc cttggcgcga
     4861 ggtgctgggc tcggcgaggg tttcgaggcc atttaccgag cagcgatcga gctaggcctt
     4921 tacccacgta cgtggaaaac gagcatcatg tatgcatcgc caaagaagcg cacaaacatg
     4981 ctgttcacgg tatgggccag gccaaccgac ggcaaactac gactctccgt gtggcatcag
     5041 ggcttccacg atttctacgg tttctctgtg gagcaagttg cccaatacct aggccccgag
     5101 ggggaccgct tcttcactgt ggccgatgcg catgagtttg cggagaagtt gcgcgctctg
     5161 tttgcagctc ttgcgccgca agcacaagaa gggcaagcag cagcggacaa gccgaggcta
     5221 gccagcaaga ctgaaacggg cggaagcggc actgaatccg acgggacgct gccgaactag
     5281 gtcgtttctg ctgacgtggc cctcgctgcg ctcgggacca cgcagcagaa cgccggtcgt
     5341 tcggcagaca ctttcgagcg gcgtggcacg agcaattccg ttggcgagtt ggagaaacgc
     5401 agctcgctga atcgcgggtg cctgaccgtg cagcgcggaa gcagcagctt gaggatcctt
     5461 tgaacgtttg aatgaggtgg accccatctt tcggacgctc ggcacgtcat ggcgtcgaac
     5521 cggcaatgca gcgccaatcc gggcgcaccg acctttcagt ggctgcggca cttggtcccg
     5581 cgcgtctact tggcggccgt ggagcagcca tccgcgagcc actatcgtgc ggcagcggcg
     5641 gcttgggcat ggatctggtc gtatggttct tgcggcgcac gcccgatctg ggcacattgg
     5701 tcttgggtcc tccgcagctt tgctttggga tcgatgtgaa tttgcccaat gccgaccagg
     5761 ccatcattcc cctcgagaag gtccgtgact atttgctttc ggcggccaat ccgcgtgcac
     5821 gagggaaagc cgcgttcttc caaacacttg ggttcgatcc agtgcaatgg gaggtgcttc
     5881 gcgacgcgct actcgaagtc gcgcggacgg gagatgccag ccctggccaa cgcagcgaat
     5941 tcggaacgaa atacgagatc cgtgctacca tctgcggacc aactggccgc cgtgccgtca
     6001 tccggacggt gtggattgtg aacgccggcg aagacagacc tcgcttcatc actgggtatc
     6061 ccgactaatg gccactgagc tccaccaaga agtcattctc gctcgtgatg ttcctcagca
     6121 cgggctgaga gtgggcgaca ttggaacggt cgttcacatc tatcccaagg gcgggctgga
     6181 agtggagttc ttcacggcct ccggcaagac ccgtgcggtg gtcacgttgc gtgatgcaga
     6241 tgttcgaact gcaacagaca acgatgtggt ggccgttcgc aaactggacg ccactggctg
     6301 acagagccgg tccggcacgc gttcaatcgg gcgatgtgag taggcattgg gcgagcgaac
     6361 aagacaagcg tcggtatgcg cttcacggag ccgtgtagcg ggagcgccgg aagagcctca
     6421 cgtttgcggg cgtgacaaag gcacctgaat gggccgcacg ggtcgctgcc tggcgagcga
     6481 gcggcaaaac gtccaaacag ttctgcaagg agcgaggcta ctcggccagt agcctgctct
     6541 ggtggtcgag cgagctcaac cgtcgacgta cagagtcgtc gcgcgccaac cctctggcgt
     6601 tgatgcgtgt cgtgcgcaaa cgcgagccgg cagcggcgag gtctgcaccg caccattgat
     6661 cgtgcacggg gacggcgtgc gcgtcgagct ccctccgggg gcggatgcag ccctgctcgc
     6721 gctggtcgtc gacgtcgtga ggaagcgtgc cggcgcggga ggacagcgat gatccccagc
     6781 ggcgtcgagg tgttcatcgg gctcgtcccg atcgatctcc ggtggggttt cgagcgactc
     6841 gcaggtcttg ctcaggagca catcggccgc tccgcgcgca gcggagcgct cttcgtcttc
     6901 gtgggcaagc gaaagacggc tctgaagatt ttgttcttcg acggctccgg aatgtgcctg
     6961 ttctacaagc ggctcgaccg ccgcacattt cgtgttcccg aagtgccgag tcccggtatc
     7021 ctgagcatcg agatgggcga gcgtgagctc gatgacttgc tcgatggcat cgacgtggag
     7081 ccagagtcca caccgccgcg cgctcgcaag cgcttgcact gagaaagtac ggcgccgtcg
     7141 cgaaaggggc tagacaaggc gctctcgaat gagcacgaat cttctcgtgc gtggcagcga
     7201 tgtagatgtt ctggacccac gcttcgcagc gcatccagag ctctacgcgc tgctggaacg
     7261 cgagcggact cagtgggcgc gagagcgcgc gtcactggcg gagcacatca cgctgctcga
     7321 gcaggagcgg gatcgcctgc gcgcgtccca cgagcggcta cgccaggagc tggagctctt
     7381 caagcgccgg ctgttcatcg ccaaggccga gcgcatcgac actcggcaac tggagctcga
     7441 gtacgctcag aagctccgcg agctcgacac cctcgcgggc acgctcggtc tcgccaaaga
     7501 gcccgcccag gccgaggacc ctacgcccgc cagcgatggc aaacgccgcg gcaagcggaa
     7561 gggcaatcgc ggcacggggc gacgagatct gcgcttgctg tcgctcgagg aagagcgcat
     7621 cgagatcacc gacccccatc tggaagccct cgttgccgag gggaaggtcg ttcgccacgg
     7681 cttcgaggaa acttacaagc tcggccacaa acgcggtgga cgccgccgca tcgtgatcgc
     7741 tcgcgtccgc tacaagaccg tcgatgccaa cggcgacacc gacgtgatca ctaccgccat
     7801 gcccgagcaa atgctgccca gcgcgcttgc cgctccttcg ctcgtcgccc acgtcatcat
     7861 ggaaaacgtc ggcaagggca tgccgctgtt ccgcatcgag gacggtgcgg cgcgcgacgg
     7921 catcccgctc gaccggggta gcctgtcgcg gtggaagagg gccgtcggcg agcgactggc
     7981 cagcaccgtt gtggaggcca tgtggaagca cgcgctggcc actgcgttct gcatctccac
     8041 cgatgccacc ggcatctgca ttgggtgcag atcctttaga acggtcgtaa attcgacggt
     8101 ttatgccact ttctgattag aaagcagggg ccctcgggtt acgtatgagt tgcaagacaa
     8161 catacgaccc aaaggcccca tgaaggcagc gtacagcga
//
LOCUS       DASTKU010000469         9158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1875195, whole genome shotgun sequence.
ACCESSION   DASTKU010000469 DASTKU010000000
VERSION     DASTKU010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875195"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VFQ61_16290"
     CDS             complement(<1..468)
                     /locus_tag="VFQ61_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9956068.1"
                     /translation="MSTQRPPVATYRIQLRQGFGFDEAAALVGYLAELGISHVYLSPC
                     QRSAPGSTHGYDVTDPTRVDEDLGGEEARQGFLAALTRAGLEHMVDIVPNHMSVASEE
                     NRWWWDVLENGPYSAHAQTFDVEFDGPTERSKDKVLLPILGSHYGRALEASQIQ"
     gene            992..2584
                     /locus_tag="VFQ61_16295"
     CDS             992..2584
                     /locus_tag="VFQ61_16295"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9956069.1"
                     /translation="MTESHSWWQKGILYQVYVRSFCDASGDGVGDLAGIRQKLDYLQW
                     LGVDAIWLTPFYPSPMADFGYDVANYTDVDPLFGSLEQLDQLIAEAHARNLKVIIDFV
                     PNHTSDKHPWFVESRSSKDNPKRDWYIWQDPVDGGPRNNWLSAFGGSAWEYDEATGQY
                     YYHAFLKEQPDLNWRNPQVRAAMYSAMRFWLDRGVDGFRVDVLWHLIKDAQFRDNPVN
                     PDYVQGDIPYNSLVPAYSTDQPEVHEIVREMRQLVDSYPNRVMIGEIYLPIRRLVAYY
                     GDAQDGAHLPFNFQLITLPWSAVQIGAAISEYEGALLAWAWPNWVLGNHDQARIASRV
                     GLPQARVACLLLLTLRGTPTMYYGDEIGMRDAPLSPEQYQDPQGKNVGVSRDPQRTPM
                     QWAAAEYAGFSQVPPWLPVSPDYAQRNVEKERHDPESILNLYRRLIELRRNSPALCVG
                     SYTPLVTQGQLLAYLREAGNERFLVVLNLGHGPQQFRLDNFGEAEVVVSTDTAREGQR
                     VKDVLRMVGDDGFVLRLKPKQD"
     gene            complement(2771..4252)
                     /locus_tag="VFQ61_16300"
     CDS             complement(2771..4252)
                     /locus_tag="VFQ61_16300"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9956070.1"
                     /translation="MKSRYDVVVVGSGPNGLSAAVALANSGLSTLVVEAAATPGGGAR
                     SLPLTLPGVLHDVCSTVHPLGAASPFFKSLDLERYGLSWVHSPAVLAHVLPDGHVVTL
                     ERSIQQTAKQFPHDGARYADLMTPLAAEYDALVGSFLGPLRFPRPTRTLFNFGLSALR
                     SMRGLLNSRFRDREARGLLAGIAAHAMLPLTAPATASFGLVLGAAGHAAGWPLALGGS
                     QVITDALLSCLEERGGELELEFRVRNLNQLPEARAYVFDLGPRQLLSIAGEQLPMSYH
                     RRVQRFRYGPGVFKIDWTLREPVPWLNPACARAATVHLSGDFEDIAESERAVHEGRMP
                     ERPFVLFVQPSLFDATRAPEGLHIAWAYCHVPHGSGVDATEAIEREIERFAPGFRSSI
                     LARASKNAQQMEYQNPNYVGGDINGGQANLAQLFFRPIVSLDPYSTPAPNIFLCSSST
                     PPGGGVHGMCGYFAARSVLNRVFSTTLSPSLELGAHPDLSIAT"
     gene            complement(4249..5319)
                     /locus_tag="VFQ61_16305"
     CDS             complement(4249..5319)
                     /locus_tag="VFQ61_16305"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9956071.1"
                     /translation="MSALLGRVVVITGASSGLGRAAAVEFAKRGCALVLAARRAEELE
                     RTAQDCRSLGGPAWCIPTDVTSEVQVLRLAARTLELLGRIDVWVNNAGVTVFARLHEV
                     PFREHQRVLDTNLNGAMYGARAVMPIFSRQGHGTLINVGSIVSKIGQPFVPSYAVSKF
                     ALRGLSEVLRVEYADHPKIHVCLLMPYAIDTPHFESGANHLGRQPHAMPPVQSPEQVA
                     QALVELAERPRRERHVPRLAWLGLVLHELMPRTIERVLLRALSRWHFAQRLEPDKPGN
                     LLCPAPSPGQVHGTRPPLVSTARLFAWAATELVRIRLRDLAFWLSRAAAWATRAAHGT
                     SSPAALRLRSAVPITPSAELGT"
     gene            complement(5434..7179)
                     /locus_tag="VFQ61_16310"
     CDS             complement(5434..7179)
                     /locus_tag="VFQ61_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956072.1"
                     /translation="MQVYRDQACSACPNDILPQLLELTDTASSVDQCRDLLIAIGEFE
                     AGVTDALCPTRDSTSPLIVRLREATLAGGLLFLAARDGDSQAQAAARASLYVTWSRLA
                     KFEFPLSIRVRPPEGFSQYCLFPEQYLRAARVFQGCFRAERALVVGLRSIGTTLSAVV
                     EASLRSSGVVTESVTLRPHGHPFDRKLEVCSELEARLAGFARTGGWVLVVDEGPGLSG
                     SSLTCVVDYFQRLGARPDRVVLLPAYDSSGDQFVNERARELWRTSQRIVSHFDAGWFH
                     DSAARLVEEPNTAWRATSSFDWAVHPHHGRRRYRTKDPDPSAPSTVWRFAGFGRYGQA
                     VFARASALAEAGFSPGARQLKAGFMQLDWVPNLPANPSARSPATLSASTVSSLRPSIG
                     WLADYFAFRERRFRTEAPPPIEPLLQVLMTNARACLGEQATAALRVLERELPKADRHT
                     VVCDARPFPQDFLSSGARSWKVDAWDHGDDHFFPGPVDIAWDLAAASEELELGAAGQA
                     YLVDRYALATRDASVVHVLPWYRATYLSFRAGYCRLAADSLADETERARFERAFARYR
                     DKLARALAVSSQCVA"
     gene            complement(7819..8049)
                     /locus_tag="VFQ61_16315"
     CDS             complement(7819..8049)
                     /locus_tag="VFQ61_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956073.1"
                     /translation="MTLPNFPPLVPPVVPSPDAPDLPSPNVPLPEPPPNMPPERPQPA
                     EPPGQPAMPDPRPIDPFPYPTYRDPEEPKTID"
     gene            8566..8871
                     /locus_tag="VFQ61_16320"
     CDS             8566..8871
                     /locus_tag="VFQ61_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956074.1"
                     /translation="MSAIEAAEGFDIMRLERDRVSASRRKRAFRAALGSSLILAGTLP
                     LGRLRTIPILLGAGLLIKEFAQDTVGLLTPSKSPSERRDLVDEASWQSFPASDPPSY"
BASE COUNT         1774 a         2804 c         2902 g         1678 t
ORIGIN      
        1 ctgaatttgg ctcgcttcga gcgctcggcc atagtgtgag cccaagatcg gcagcaagac
       61 tttgtccttc gatcgctccg tggggccgtc gaactcgacg tcgaaggttt gggcgtgcgc
      121 gctatatggc ccgttttcca gcacgtccca ccaccatcgg ttctcctccg acgcaacgga
      181 catatgattg gggacgatgt ccaccatgtg ctcgagtccg gctcgggtca gcgcggccag
      241 aaagccttgg cgtgcttctt ctccacccaa atcttcgtcg acgcgagtgg gatccgtgac
      301 gtcgtagccg tgagtactgc cgggcgccga tcgttggcaa ggcgacagat agacgtggct
      361 tattccgagc tcggcgaggt atccgacgag tgccgcggct tcgtcgaacc cgaagccttg
      421 gcgcagctgg atccggtagg ttgcgacggg agggcgttgt gtagacatgg gaaaacagcg
      481 gttctgcaac gaacatgccc ctcaagccct ttgctaaggt cttccgcttc gctggactca
      541 gtatctcgct tgctgtttct cgagtccaag cgttcgtggt tagccgtatt gagtcggagt
      601 ccagagctcg ttcttctttg cgcagctgac atcgcgcgcg atcctagaag ctcgactgag
      661 tgatctcagc caggtcatta ataccggtcg cgcaatacca gtcgcgtcat ctcagccagc
      721 tcggtcacaa ctctagcgat gctccctatc gcgagcgccc cctggcaaga cgtcaatcgg
      781 ccgcccagag tgtgttgcat tcgatagggt gatcttaccg ggcgggctct cggaaagttg
      841 gcgcatcctt gagcatggat tgtgctgggt atcgcgcagc gtcgcgcatg aagccgttga
      901 aagctttact ttaatagaca acgtggcgcg cggccgggcc gcggctgccc tgtggcagca
      961 gtgctcggtg tggcggatct acgaggtgaa catgaccgaa tcgcactctt ggtggcagaa
     1021 ggggattctt tatcaggtct acgtgcgctc gttttgcgac gcgagcgggg atggcgtggg
     1081 tgatctagcg ggcatacgcc aaaagctcga ttacctgcag tggctgggcg tagacgccat
     1141 ctggctgacc cccttctacc cgtcaccgat ggccgacttc ggctacgacg tcgcgaacta
     1201 cacggatgtg gatccgctct tcggttcgct ggagcagctc gatcagctga tcgccgaggc
     1261 tcacgcccgg aacctgaagg tcatcatcga cttcgtgcca aatcatacgt cggacaagca
     1321 tccgtggttc gtggagtctc gcagctcgaa agacaatccg aagcgcgact ggtacatctg
     1381 gcaggatccc gtcgacggcg gcccgcgcaa caattggctg agcgcgttcg gtggctctgc
     1441 ctgggagtac gacgaagcga ccgggcagta ttactatcat gccttcttga aggagcagcc
     1501 ggatctcaac tggcgaaacc cgcaggttcg cgccgcaatg tactcggcga tgcgcttttg
     1561 gctcgaccgg ggggtcgacg gattccgtgt cgacgtgctc tggcacctga tcaaggacgc
     1621 ccagttccgc gataacccgg tgaatccgga ctacgttcaa ggagacatcc cctacaactc
     1681 gctcgttccg gcatattcta cggaccagcc cgaagtgcac gagatcgtcc gggaaatgcg
     1741 tcagctcgta gactcgtatc ccaatcgggt catgatcggg gaaatctacc tgccgatacg
     1801 acgcctcgtc gcctactatg gcgatgccca agatggcgcg catctgccat tcaacttcca
     1861 gctcatcacc ttgccttgga gtgcagtaca gattggcgcg gcaatcagcg agtacgaagg
     1921 cgcgctacta gcctgggcct ggccgaattg ggtgctcggg aaccatgatc aagcgcgcat
     1981 cgcgagccgt gtgggtctac cgcaggcgag ggtcgcgtgc ctattgttgc tcacactgcg
     2041 gggcacaccg acgatgtact acggcgacga gatcgggatg cgagacgcgc cactctctcc
     2101 ggagcagtat caagatcctc agggtaagaa cgtaggagtg agccgtgatc cgcagcgcac
     2161 cccgatgcaa tgggcggctg cagagtacgc cggattcagt caggtaccgc cttggcttcc
     2221 cgtttcaccc gactacgcgc agcggaacgt ggagaaggag cgccacgatc cggagtcgat
     2281 cctgaatctg tatcggcgcc tgattgagct gcgccgcaac tcaccggcgc tttgcgtcgg
     2341 ctcctacaca ccgttggtca cccaggggca gctccttgca tacttgcggg aggcggggaa
     2401 cgagcgattc ttggtcgtgc tgaacctggg tcacggcccg cagcagtttc gcttggacaa
     2461 cttcggagag gcggaggtgg tcgtctccac cgatactgcg cgcgaggggc agcgcgtgaa
     2521 agacgtgctg cgcatggttg gcgatgacgg cttcgttctt cgattgaagc ccaaacagga
     2581 ctagtctggt tcgttaccta tccactagtt cttcgggaac ctagtccaga ctaaacccgc
     2641 ggcgaccgct cagattgatt acacgatggg atagcctagc tcgcaaccgg cgagttaggc
     2701 cgtccgatcg agtgctagcg cccacggcgc ctcgtgaccc tatgagcacc ggttcgattt
     2761 acgatgctat ttacgtcgcg atgctgaggt ccgggtgcgc tccgagctcg aggctcgggg
     2821 ataaggtggt gctaaatacg cggtttagga cgctgcgtgc cgcgaagtat ccgcacattc
     2881 catgtacacc gccgcccgga ggtgtggacg acgagcacaa gaatatattc ggggcgggcg
     2941 tggaataagg atccaggcta acgatgggtc ggaagaacaa ctgagctaga tttgcctgcc
     3001 caccattgat gtccccgccc acgtagttcg gattctgata ctccatttgc tgcgcgttct
     3061 tgctggcgcg cgctaggatc gagctgcgaa acccgggcgc aaaacgctcg atttcccgtt
     3121 cgatggcctc ggtggcgtcc acgccggagc cgtgtggaac gtgacagtac gcccaagcaa
     3181 tgtgcagccc ttcgggcgcc cgcgtggcat cgaacaagga gggttgtacg aagagcacga
     3241 acggacgctc tggcattctg ccttcgtgca cggcgcgctc gctctcggcg atatcttcga
     3301 agtcgccgga tagatgcacg gtggcggccc gggcgcaggc cgggttgagc cacggcaccg
     3361 gctcgcgtag ggtccaatca atcttgaaga cgcccgggcc gtaccgaaat cgctgcaccc
     3421 ttcggtggta actcatgggc agctgctctc ccgcgatgct gagcaattgc cgcggcccga
     3481 ggtcgaacac gtaggcccgg gcctccggca attggttcag gttgcgcact cggaattcga
     3541 gctccagctc ccctccgcgt tcctccaaac aggaaagcag cgcgtcggtg atgacctgag
     3601 accctcccag cgcgagcggc caacccgcgg catgacctgc ggcacctagc accaagccga
     3661 aagacgcggt ggccggcgcg gtgaggggca acatagcgtg ggcggcaatt ccggccagca
     3721 gccccctcgc ttcacgatcc cgaaagcgcg agttgagcag acctcgcatg gatcgaagcg
     3781 ccgaaagacc gaagttgaac agggtgcgag tcggacgtgg aaagcgtagc ggaccgagaa
     3841 acgatcctac cagcgcgtcg tactccgcgg cgagcggagt catcaagtct gcatagcgag
     3901 cgccatcgtg cggaaactgc ttggccgttt gctggatcga tcgctccaaa gtcacgacgt
     3961 gaccgtccgg taacacgtgc gcgagcacgg ccggagaatg aacccagctc aagccatagc
     4021 gctcgagatc gaggctcttg aagaacggag aggccgcgcc caacgggtgt actgtagagc
     4081 agacgtcgtg caagacaccc ggcagcgtga gtggcaaaga gcgcgctccg ccccccggcg
     4141 tcgccgcggc ttcgaccacc agtgtagaaa gtcctgagtt ggcaagggcg acggcggccg
     4201 agaggccatt cgggccggag cccaccacca ccacgtcata tcggctcttc atgtgccaag
     4261 ctccgcgctt ggagttatcg gcaccgcgct tcgaagtctc agcgcggctg ggctactcgt
     4321 tccatgcgct gctcgcgtgg cccatgcggc ggcgcgactc aaccaaaacg ccaggtcgcg
     4381 gagtcgaatc cggacgagct cggtggcggc ccaggcgaac agacgcgcgg tgcttacgag
     4441 cggagggcgt gtgccgtgca cttggcctgg gctcggggcg gggcacagca ggtttccggg
     4501 cttgtcgggc tcgagccgct gagcgaagtg ccagcggctc agagcccgca acaggacccg
     4561 ctcgatggtg cgtggcatca gctcgtgcag caccagtccg agccaagcca atctcggcac
     4621 gtgtcgttcg cgacgaggtc gttcagcgag ctccaccaag gcctgtgcga cttgctcggg
     4681 cgactgtact ggcggcatcg cgtgaggctg ccgacccagg tgattggccc cagactcgaa
     4741 gtgcggggta tctatggcat acggcatcaa caagcacacg tggatcttgg ggtggtcggc
     4801 gtactcgacc cgtaagacct cgctgagtcc gcggagcgcg aacttgctca ccgcgtacga
     4861 gggcacgaag ggttgcccga ttttgctgac gatggagccg acgttgatca gagtcccatg
     4921 cccttgccgt gagaagatcg gcatgacggc ccgcgctccg tacatcgcgc cgtttaggtt
     4981 ggtatccagc acccgttggt gttcacgaaa aggcacctcg tgcagtcgcg cgaacacggt
     5041 gacgccggcg ttgttcaccc agacgtcgat acggcccaac agttcgaggg ttcgcgcagc
     5101 cagacgcaga acctgaacct cggaggtcac gtcggtagga atgcaccacg cggggccgcc
     5161 caggcttcga cagtcctgcg cggtgcgctc cagctcttcc gcgcgacggg ccgcaagcac
     5221 gagagcgcag ccgcgcttcg caaactccac tgccgccgcc cgcccgagcc cgctcgacgc
     5281 acccgtgatt accacgaccc gacccaaaag tgcgctcatg ctgcgtcctc gatcatcccc
     5341 tcgaagggat gcaaagggcg tgcagagctg cggggcacaa ccacagagca gaaccacaca
     5401 gagccgctcc gctcattgtg tctttgcctg cgttcacgcc acgcattgac tggagaccgc
     5461 gagagcgcgc gcgagcttgt cgcgatatcg cgcaaaggcg cgctcgaagc gagcgcgttc
     5521 cgtttcgtcc gccaggctat cagcagccag gcgacagtag cctgcccgga acgagagata
     5581 ggtcgcgcga taccagggca gcacatgcac gacgctcgcg tctcgggtgg cgagggcgta
     5641 tcgatcgacg agataggcct gaccggcggc gcccagctcg agttcttcgc tggcagctgc
     5701 gagatcccac gcgatgtcca ccggcccggg gaagaagtgg tcgtccccgt gatcccaagc
     5761 gtcgaccttc caggatctgg cccccgagct caggaaatct tgggggaacg gacgcgcatc
     5821 acacactacc gtgtggcgat ccgctttggg aagctcgcgc tcgagaacgc gtagtgcagc
     5881 agttgcctgc tctcccagac aagctcgcgc gttggtcatc agcacctgaa gcagcggctc
     5941 aatcggtggg ggggcctcgg tgcgaaagcg acgctcccga aatgcgaagt agtcagcgag
     6001 ccaaccaatc gaaggacgca ggctagaaac agtcgatgca gatagcgtag cgggagaacg
     6061 tgcagagggg ttagcgggca ggttcggaac ccagtcgagc tgcatgaaac cagccttgag
     6121 ctggcgggct ccgggagaaa agcccgcttc ggccagagcc gaagcgcgcg cgaacaccgc
     6181 ctggccatag cgaccgaagc ccgcaaaacg ccagaccgtg cttggggcac tcggatcggg
     6241 gtccttggtt cggtagcgcc gtctcccgtg atgcgggtgc acggcccaat cgaacgaact
     6301 agtcgctcgc caggccgtgt tcggctcctc taccagtcga gctgccgaat cgtggaacca
     6361 gcccgcgtcg aagtgagaga cgattcgctg cgaagtgcgc cacagctcgc gcgccctttc
     6421 gttgacgaac tgatcgccac tcgagtcata ggcgggcaac aagacgacgc gatccggcct
     6481 agcgccgaga cgttgaaaat agtcgacgac gcaagtgagc gacgagccgc tcagccccgg
     6541 gccttcgtcg accacgagca cccagccgcc cgtccgcgcg aagccagcca atcgggcctc
     6601 caactccgag cagacctcta gtttccggtc gaaagggtga ccgtgagggc gcagcgtgac
     6661 cgattcggtt acgactcccg agctgcgaag agaagcttcg accaccgccg acaaagtggt
     6721 gccgatgctg cggaggccta cgacgagcgc ccgctcggcg cgaaagcaac cttgaaacac
     6781 ccgcgcggcg cgcaagtact gctcgggaaa caggcagtat tggctaaatc cttcgggcgg
     6841 gcgaactcga atcgagagcg gaaactcgaa cttcgcgagt cggctccaag tcacgtaaag
     6901 tgaggctcgc gccgctgcct gagcctgcga gtcgccatca cgtgccgcga ggaacaacag
     6961 cccacctgcg agcgtcgcct ctcgaagccg cacgatcagc ggcgaggtcg aatcgcgggt
     7021 tgggcagagt gcgtccgtta cgccggcttc gaattcgcca atcgcgatca ggagatctcg
     7081 acactgatct acggacgaag ccgtgtcggt cagctccagg agctgaggaa gaatgtcatt
     7141 cggacacgcg gaacaagcct gatcccgata aacttgcata ttggcggcgt tcgctcgaac
     7201 tatctattgc atctgcaatg cagaaagcgc taaccgcccc gcctacgggc ctccgccacg
     7261 cgcggcctca tagcatgatc cgcgtcattc gcgcgggctg ccgcgcatgc ggaacaaagc
     7321 caaatcggct catccgggca tgacgcgcgt gagtccgtca tccagccttc gcgagcgagc
     7381 ggaacaaagc cccactcggt tggctctgac cggacatggc atatcgagca ctttagcggg
     7441 gcaatactca tgggtgggtt tgattacgct atgcaagcca ggtgcccgga gccggctcgc
     7501 gcgcccgatg tccgtgtgat cgacgcctac ttcccagaaa acgatatgag cagctctcgc
     7561 agcggtaggg tcgaagtccg aacaccttga gaagtttctc cgtccaccca cgagtggcgg
     7621 aacgatgata gtccgagtcc ccacagcgag ggcatttgac ttgcgccatc tacccacgag
     7681 tctctgcatg gcccatgccg gtttccgcca ggcgcgaggc actttgcttc gactatgttg
     7741 gattgaacgc aaatcactgg tccgggcgag ctagcatttc ggtcactggg tgagttggcg
     7801 caccccgaga gccgtcgctt agtcgatcgt cttgggctcc tcgggatcgc gataagttgg
     7861 ataagggaac ggatcgatgg gcctcgggtc cggcatcgcc ggctgacccg gtggctcggc
     7921 aggttgaggg cgttccggcg gcatgtttgg cggaggttca ggcaagggga cattcggtga
     7981 aggcagatcc ggcgcgtcgg gtgagggcac aaccgggggc acgagtggcg ggaagttggg
     8041 aagtgtcatg gggcgattct ccactcagga gctctgcgga gctcatctcg aggccaccgc
     8101 aagtccagat cttctcgagg tttacgaact ggttacgagc acggatccgt gagcacgtgc
     8161 cgtgccgagc tcgccccaga cggagctgtt ttgctgagtg gctgttgaaa acagccactc
     8221 taaccaagaa ctcggggttt acacggacgc cctcgtgtgg cgatcgcggt caccactagt
     8281 gatagacggt gcgattttct gctctccgcg cgtgggcccg caacgctgct cgcctgttgc
     8341 gctgctggtc ggacgctatg gcgcttccgc agggcagttc gcgtgtggcg cgacgattgc
     8401 agttgattcg actgcattgg atcaaccaag cttcgttctc gcacgcgcgt ctcacgcgcg
     8461 cacgttctac gcgcgccgag gaaggcgcgg cgtgatcgag tcgatcggat tcactccgtg
     8521 gccgacactg ggcgaggcaa cgagcgagtc aacaggagga gcgatatgag tgcaattgag
     8581 gcggctgaag gtttcgacat catgcggctc gaacgtgacc gggtatccgc gtcccgtcgg
     8641 aaacgcgcgt ttcgggcggc cctgggtagc agcttgatat tggcgggaac gttgcctctc
     8701 ggccgactgc gaaccattcc gattctgctg ggcgcaggtc tgctcatcaa ggagttcgcg
     8761 caggatacgg ttggactgct cacgccgtcg aagtctccct ccgaacgccg agacctggtg
     8821 gacgaggcgt cctggcaatc ctttccggcg agtgatccgc cctcgtattg atggctcgcg
     8881 tatcgacgtt cgaacacgtc agcactaacc atgtcactac ctaacgcaca gccgcggagc
     8941 gatcggggcc gacgttcctc tgctagttcg cgcctctcgg cttgtcttcg gggcgctcgt
     9001 tcggcgggcg cggcgatatc cttcgccctg ctgctacttg gcggtttggg cggcgcatgt
     9061 cgcagcccag acgaaccgcc taaatcgccg cccagctcac cgccaagtaa gcccgatggc
     9121 aatccccccg cggtggagcc cgatacgggt caaccgat
//
LOCUS       DASTKU010000470        16619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_981365, whole genome shotgun sequence.
ACCESSION   DASTKU010000470 DASTKU010000000
VERSION     DASTKU010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16619
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981365"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            603..1724
                     /locus_tag="VFQ61_16325"
     CDS             603..1724
                     /locus_tag="VFQ61_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9956075.1"
                     /translation="MRNVVPHRRFEVGRPPARLLLVFAGVFCASFPALADEQAPPPAG
                     SSREEAAATGPHSRPVTTIENERAWTRGVSEAAQEQARTLFAQANAHLKDSFFQQAAT
                     EYRQALALWNHPGIHYNLALALLNLDQPLEVREHLLQAVRYGSAPLDEDKYEYAQSYL
                     KLLEQQLAPIEIRCSVPGAIVKLDDAHLFHAPGSYKGYLRPGRHRIQATKPGFATNEQ
                     VRTLLPGEPTRVDLRVYTEDELTRYRRRWPVWAPVTVIGGGVALAAAGGIFLMQSKRT
                     FEEFDHKVDKTSDCIDDGCQLSPSLSDLRNRANTYQTLGGIGLGVGSAALVTGTVLLF
                     LNAPVAYRVEPEDLSARRGLRIEPRIGSGFAGISARGEL"
     gene            1724..4108
                     /locus_tag="VFQ61_16330"
     CDS             1724..4108
                     /locus_tag="VFQ61_16330"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9956076.1"
                     /translation="MFTMRRFGGGSFLLMAALLASACYSSEREECRDGRVCAIGMRCT
                     ADGDGCTATKCGDGIVDRDAPDEEACDDGNVRDGDGCSKDCKSVEKCGNGIRDLGFGE
                     GDPRNEECDQGASTEAGELGPRNTEDCDSDCTKPACGDGHFNPNARTEFGNFEQCDDG
                     LLNDDTVYGDPSRCTTTCKKAPFCGNGLTERGEECDPGLDSEGRPQETEACNLSADGR
                     ADPRNGRETDCRKSRCGDGYVNETTEACDPGSETETCNLATERRDTNCQEARCGDGYI
                     NSAAHEVCDLGEKNDTDVVYNNRMGCTPDCLIPPFCGDKIVSPEYGETCDPGQEDAPE
                     NPCREDCTFCGDGLKNGDEDCDAGRPEGSDSCSPTCQNRLCGNGRVDPGEACDDGNDD
                     NTDGCVIIGPDASQCQRPRCGDGFTLQAAFADPEHGLLPEQCDDAGESAGCDFDCSLA
                     ECGDGLINRKNLEVCDEGKNGSKHCTNTCQPSFCGDGVHNPLSEQCDPTVPGSISPGG
                     RCDSDCTYQHCGDGRISRSLDGTQLEECDGGGGDTAVDTQTCTSECKLSFCGDGHVNS
                     LTEECDDKNPTSGCNPITCKWSKCGNHSVDFDETCDEGANGVPRNTADCDADCSPAKC
                     GDGFFNPENIIYSGPNREQCDDGNGNSADGCSYSCQLEAGYECPTPGKPCRTICGNGT
                     LNTNEACDDGNTKSNDGCTNCQIDAGFECPGGKSCRRLCGNGTLNSPEACDDGNERSD
                     DGCSNCQLDAGFECLQPGKPCTLLCGNGHIEVPEECDDGNDLPSDGCTNCKKDP"
     gene            4169..5191
                     /locus_tag="VFQ61_16335"
     CDS             4169..5191
                     /locus_tag="VFQ61_16335"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9956077.1"
                     /translation="MPSYRFEREVTWAGLATLFLFQLVACGETSDPNRGSDCSGAACA
                     GRGGGYTGGRSMAHSGAGNSGEGSGGAGGSSGETEASSGGAPVVSSGGEKNTGEGGAE
                     GGARPEGGTSHGSDPGLCGDGKRTTGEECDDGNSQDDDDCTNQCKRALCGDGVTQTRA
                     SSPEACDDGAESATCNEDCSEARCGDGIVNASAKEACDDGQDTDRCNTDCTPAKCGDG
                     YTNRAAGEACDDGRETEQCNADCTISYCHDGRVNATDGEECDPGDDDETDECDADCSE
                     ARCGDAHVNQAAGEECDVAGISATCDGDCTRPVCGDGYPNFAVEQCDDGNTRNGDGCS
                     SACKLE"
     gene            complement(5237..6385)
                     /locus_tag="VFQ61_16340"
     CDS             complement(5237..6385)
                     /locus_tag="VFQ61_16340"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET9956078.1"
                     /translation="MVQRAAEQFASVGVAENGNSAVLVTLAASGEVLDRRTVDLTESG
                     LPTHPHHHEGSWAVGRYLNTPWAREISLPEAVKLVERVQEAAGKGARQRLQELSEAVE
                     VPITAIAIRVCPALPATIEQRIRDNRAQTYADSVMYRQAIAAAAESRGWRVHWYEVES
                     VVSEATLRVDMLQVDNADLETFLHDLGRTLGPPWQSKHKLAAAAALAAQAGARQGKGN
                     VKGTTVTRKKATVKKRQPTQKAAAGRASAQKARLLSGDNPQISKADGDAPVQAYIAAI
                     PDWKRDVARYLDALIVRTVPGVRKAVRWNTPFYGLEGKGWFVSFHCFTRYMKVTFFRG
                     AALEPLPPVESKQKHVRYLHIHEAEELDESLLVSWLEQASELPGEALF"
     gene            complement(6531..8009)
                     /locus_tag="VFQ61_16345"
     CDS             complement(6531..8009)
                     /locus_tag="VFQ61_16345"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9956079.1"
                     /translation="MRRKFLLLSLVFGVSCSAYSGDAEQVDAKQSEIVRGNNEPNRPY
                     VVMIHSEIYGGSVTRCTGTLVAPRVVLTAAHCLRNGRWWHGTFVYYGKDYASDLSLLN
                     KRPIGPSTIGPQSEQNVWAMAEADEAFPSYDANTHYPDLAVLYLDRKPPIDPLPIYRA
                     RLDDSWIGKKGTLVGWGANKALSADIKQVEGGGVKRSAQVPILGTPTAADYHEDDPNP
                     GMLDAAIRSHWIKTSGLAPKANTCAGDSGGPMIINKWGQDYVAGVSMWTGLWCEDYSI
                     FTRLESFLPFLDDAYFKSGQAPVVPRLECVEDNADGTYTALLSYRNDNAITIQVPYSQ
                     DRNYLPLDTEGVRPSSFLTGDHPLNFFVDFTNGQPFYWKLSPTNSPTTELRASSSSPR
                     CARDSLGSSCLRSCRASIAAACDPPIETRSTLKQCFFNCADVEGFIPPECMESYIGAN
                     NCMADQDVNDPALWSCSEGYGGSAIGPACEPIVTELLTCMGW"
     gene            complement(8466..9404)
                     /locus_tag="VFQ61_16350"
     CDS             complement(8466..9404)
                     /locus_tag="VFQ61_16350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HET9956080.1"
                     /translation="MSVLGRLLRWFGALAVVAPVVQAIGTSSAQAYCRTRTCGRSDAP
                     ACMPDAEKCPTVGAPLFWPGESIRFLVDASGSPRRNISGEAAQAVLIESLTAWTSVFC
                     EDGTHPSISVASVDLVRDPIAVREGLTSPDEDHPTQEDVSNNVLAFVDEGWIYDSPDV
                     VALTTVTFGVKSGRIVATDIEVNSDQVDITASDSNVDFDLLSVLTHESGHVFGLADLF
                     RLGPTMNGRYAGGGDLGPRSLEPDDIAGICAIYPANRFQHESGCSCGVAGGGARLGAS
                     ALFGAGALLLALRRRKIARPQSHSGTQARMRGSSTM"
     gene            complement(9450..10451)
                     /locus_tag="VFQ61_16355"
     CDS             complement(9450..10451)
                     /locus_tag="VFQ61_16355"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET9956081.1"
                     /translation="MHFTRKSTVYVTRGDGLDAVHEVAVAVIDERGHLLRAGDPEAVV
                     FARSSLKPFQALALFPSGAARQFELSQEELAIACASHSGSDRHVAVVQGLLGKIGASA
                     GDLGCGVQWPIGMRLENRYPSEGEDSDPLRHNCSGKHAGFLAVARALEQPLAAYLEPE
                     SKVQTLVRAVLASACEIDENLLGSGVDGCSAPNYALPLRVLAQAAKRLASADASESCA
                     GLAAVRRAMLTHPLLVSGPGRFDYQLTCAFPDNLVCKAGAEAIQLIGFEDPPLGIAVK
                     VLDGAERALAPAVMAVLRRLGLSADRLPQLSERVRPAVTNARGIVTGELIASIDLET"
     gene            10657..11988
                     /locus_tag="VFQ61_16360"
     CDS             10657..11988
                     /locus_tag="VFQ61_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956082.1"
                     /translation="MRVYKTRELRSKTWAVGMLATAALVGCGSDEDQNPAAAGGQTAG
                     GQTAGGTDSSIGNSAGGSSNVTPAGSAPTFAANSEVTAPVVWGTEDAKVRINHVSNLN
                     AGTTFALIASVDGIPCSKLGLADADSTILELACTIPPGNADKAPPKLLIQQLGYTQNL
                     KTVYLEVPRGSFTLPVEECEASPTPAEGVALPQLCRADPGSIAATGRGFEGIYAGTVP
                     HVSDTQSHNFGFVAGDGTYVSGNNTLWQVEGAGWLEAGRMVPNQEPWSPLQGFSFDIA
                     EGISVKSGEFYPADATGTFIPYTGNSVLVNTYSVANALAVSMSSVAGVWKNKDSGLSL
                     SIAVEGDEGQITGSVYLTPELQTCAISGTIRLREPDTAKNLYDIALEVTQVENSANIE
                     CDTADHAKLRGLAAIDFRNVGTRLSPVYAHTLDLAAYEVDKAIFSDTLAKE"
     gene            complement(12058..14472)
                     /locus_tag="VFQ61_16365"
     CDS             complement(12058..14472)
                     /locus_tag="VFQ61_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956083.1"
                     /translation="MTSQDRVERDVREDISDPAVHPQLSQDEEASDSKLVSRRELSDA
                     AAREPLEAAGPDSNAAWIVSEGDIVAAPDAESVSEPNRNRGLHPRLPMPSANSLNAML
                     MEPDGWEDHVEMPVRILSPQGSPGAVSASSVSPVLESTVLDSHALAPHPVASLMTSAD
                     ANNESSPSPKSTPESAAPPADVPVATPPLSSALPSSALPDSTVSSSTLSTSTVPASTV
                     PASTVPSSTRSPSLGTSKLDSGARVGEARGLESKASVVSSAPVGLSELKTPAQPFAAT
                     KLKGADERSESREAPRPSEPPRPNERGSAERGNSARSVVPAKRATPIKPLTLVAPKPE
                     ANAASAAAQVPAAASPASAASSVEAGVTTPVASEPVAAEPSSTKPSLVAAAVAELAGA
                     GTGSPSANAAEGTVPDASAGPPPNVGLPVPPPPPPVIPAATRPNPSSGLSAALAAQLE
                     RVDQLLSAAERPAASSSAVASVPQAVAKPTAASEPAASETLRDEPDETTAEPEAALPK
                     TNTPWGASKSGASNIVVPPGTGLAEAKLSASGAHGMNGSNGVNGSHGMNGSIGSTGSM
                     GTPSTHGDYPPNSIVSALLGTPPPPGEVVSAAPVAGRSWHSYQPEASVWNKHSKLLIG
                     VGAVVMAFIIGRATAPSSAVPTVTKHAAVSEPAGEAKPPAQPASASPPSAASAAASPA
                     APAAASAAPAPSNAAPDGAEGKAVAAPAKPEGEAGAFDEGAASEALKATMEHAASCKH
                     PGDPSGQLQVSITFAPAGKVTAANLGGIRFARASARNCVGKLLKDVRVPAFTGPAVTL
                     KKSFKF"
     gene            15026..16045
                     /locus_tag="VFQ61_16370"
     CDS             15026..16045
                     /locus_tag="VFQ61_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET9956084.1"
                     /translation="MTKSSLLSVVPLGFPWATLDPFLFCVHHDDHYPRGNEKLGPDAS
                     LAGRRLGQDFEIKDGFRMYHGREVPGFPQHPHRGFETVTLVRRGFIDHSDSLGATARF
                     GAGDAQWLTAGQGIVHSEMFPLLERDRDNPLELFQIWLNLRAQDKLVKPFFSMLWREN
                     IPALSVPDEEGRTTQLYLVAGEYSGQRPPAPPPNSWASQPDSDLAIWTLKLAPHARFT
                     LPPARSGTQRVLYFFKGDELTIDGETVPAQHAVQVAADTESRLQCGAHPAELLLLQGR
                     PIGEPVAQHGPFVMNSRSELQQAYLDYQRTGFGGWPFPEDAPVHSREAGRFARHADGR
                     IEHMP"
BASE COUNT         3258 a         5430 c         5063 g         2868 t
ORIGIN      
        1 gtgttggcct catccgttca cgcaagcgcc cttgcgcatc gagtttgccc acggctgctc
       61 gagagccccc gttccggcgt ggtgcccgcg ccaggccagc atctaggccc agtgccgaag
      121 cgaggcgatg cccacgaacg agctgctcac ggctagagaa aattcactcc ttagagttat
      181 aaagacactc acgctcaggc ttacggagta agcagagctc cagacaccga gctgcgctcg
      241 aagcggtggc tgaggttggc gctaccttcg tcaccctcac gcggcgcgtg ccctcctgcc
      301 ctcgaaggac cgccgggggc ctaggtgaga gctaggtcga gcacgacggc ttccaagatg
      361 gccggcggcg ggccacggca ccgcgcataa acggctggaa tgccgacacc gttgcgttga
      421 gcaggcccct tcgttagaag gcgagcttcg agtaatcttt tgaccgctgc gcgtcgtcat
      481 tggtgttcac actcagccct gggagagaat gggatcagac caggcacacc ccgttcaggc
      541 cctggacgga gccccttggg ataacgtagc cacgacgctc ccgagcccga aggaagaccg
      601 agatgagaaa cgttgttccg caccgcaggt tcgaagttgg ccggcctcca gcgcggctgc
      661 tgctcgtgtt cgcaggcgtc ttctgcgcca gttttccggc gcttgccgac gagcaagccc
      721 cgccgccagc tggttcctcc cgagaagaag ccgcggcgac cgggcctcac tcgagaccag
      781 taaccacgat cgagaacgaa agggcctgga cccgcggtgt ctccgaggct gcgcaagagc
      841 aggcccgaac cctattcgct caggccaacg cgcatttgaa ggactccttc ttccagcagg
      901 cggcgaccga gtatcgccag gcgctcgcgc tctggaatca tcccgggatt cactacaatt
      961 tggcgctggc actgctgaac ctggaccagc cgctcgaagt ccgcgaacat ttgctgcagg
     1021 ccgtccgtta cgggtcggca ccactcgacg aagacaagta cgaatacgcg cagagctact
     1081 tgaagttgct cgaacagcag ctcgccccaa tcgagattcg atgctcggtg cccggcgcaa
     1141 tcgtgaagct ggatgacgcg catctgttcc atgctcctgg cagctacaag ggttacctac
     1201 gccctggtag acatcgcatt caggcgacga aaccaggctt cgcgaccaac gaacaagtcc
     1261 ggacgctgct gcccggcgag cccacacgcg tggatttgcg tgtgtatacg gaagatgagc
     1321 tcacgcgcta tcgtcggcgc tggccggttt gggcccctgt caccgtgatc ggtggtggag
     1381 tcgcgctcgc cgccgccggc gggattttcc tcatgcaaag caaacgcacg ttcgaagagt
     1441 tcgaccacaa agtcgacaag acctcggact gtatagatga tggctgccag ttgagcccat
     1501 cgctctcgga tctgcggaac cgggcgaaca cgtatcaaac cctcggcgga atcggcttgg
     1561 gtgtgggcag cgcggcgctg gtaacaggga cggtgctgtt gttcctcaat gcgcccgtag
     1621 cgtaccgcgt agaaccggaa gacctgagcg cccgtcgcgg gctccgcatc gagccccgta
     1681 tcgggagtgg cttcgccggg atcagcgcgc gaggtgagct gtgatgttca cgatgcgtcg
     1741 cttcggaggg ggcagcttcc tgttgatggc cgcgctgctc gccagcgctt gctacagctc
     1801 ggaacgtgaa gagtgccgtg acggccgggt ttgcgcgatc ggcatgcgtt gcaccgcgga
     1861 cggggatggc tgcaccgcca ccaagtgtgg ggatggcatc gtcgatcgcg atgcgcccga
     1921 cgaagaggct tgtgacgacg gcaatgtccg tgacggagac ggctgctcga aggactgcaa
     1981 gtcggtcgag aagtgcggca atggcatccg tgatctcgga ttcggtgagg gtgatccccg
     2041 aaacgaggag tgtgatcagg gtgcctctac ggaagccggc gagctcgggc ctcgcaacac
     2101 cgaggattgc gacagcgact gcaccaaacc cgcctgcggt gacggtcact tcaatccgaa
     2161 cgcgcgtact gagttcggca atttcgaaca atgcgacgac gggctcctga acgatgacac
     2221 cgtctacgga gatccgagcc gatgcacgac aacctgcaaa aaggcaccct tctgcggcaa
     2281 cggcttgacc gagcgtggcg aagagtgcga tccggggctc gattccgagg gccgaccgca
     2341 agagaccgaa gcctgtaacc tcagcgcgga tggtcgtgct gacccgcgca atggtcgcga
     2401 gaccgactgc aggaaatccc gctgcggcga cggctacgtg aacgagacga ccgaggcctg
     2461 cgacccgggc agcgaaacgg agacctgcaa cctcgccacc gaacggcgcg acaccaattg
     2521 tcaggaggcg cgctgtggcg acggatacat caacagcgcc gcccatgagg tctgcgatct
     2581 cggtgaaaag aacgacaccg acgtcgttta caacaaccgc atgggttgta cgccggactg
     2641 cctgattccg ccgttctgtg gagacaagat cgtgagtccc gagtacgggg aaacctgtga
     2701 tccgggtcaa gaagacgcgc cggagaaccc ctgtcgggaa gactgcactt tctgcgggga
     2761 tggcctcaag aacggggacg aagactgcga cgccgggcgc cccgaaggca gcgattcttg
     2821 ctcacccact tgtcagaatc gcctgtgcgg caatgggcgc gtggatccag gtgaagcctg
     2881 cgatgacggc aacgacgaca acaccgacgg ttgcgtcatc atcggaccag acgcgagcca
     2941 gtgccagcgc ccacggtgtg gggacggctt cacgctgcaa gcggcgttcg ccgatccaga
     3001 acacggcctt ctccccgagc agtgcgatga cgccggagag agcgccggct gcgacttcga
     3061 ctgcagcttg gccgagtgtg gagacgggct catcaaccgc aagaatctag aggtctgcga
     3121 cgagggcaag aacggctcca agcattgcac gaacacttgt cagccgagct tctgcggcga
     3181 cggagttcac aacccgctga gtgagcaatg tgaccccacc gtgcccggct cgattagccc
     3241 cggaggccgc tgtgatagcg actgcacgta tcagcactgc ggggatgggc gaatttctcg
     3301 ctccctggat ggcacgcaac tagaagagtg tgatggcggc ggcggagaca cggccgtcga
     3361 tacccagact tgcacctccg agtgcaagct gagcttctgc ggagacggtc acgtgaactc
     3421 cttgaccgag gagtgtgacg acaaaaaccc cacctctggg tgcaacccaa tcacctgcaa
     3481 gtggagcaaa tgcggaaatc actcggtcga cttcgatgaa acctgcgacg aaggcgcgaa
     3541 tggcgtacct cgcaacaccg cggactgcga tgcggactgc agccctgcca agtgcggaga
     3601 cggcttcttc aacccggaga acatcatcta tagcggcccg aaccgggagc aatgcgatga
     3661 tggcaatggg aacagcgccg acggttgctc ctacagctgc caactcgagg ccggatacga
     3721 gtgccccacc cccggcaaac cgtgccgtac gatctgcggc aacggaacgc tgaataccaa
     3781 cgaagcctgt gacgacggga ataccaagtc gaatgacggt tgcaccaact gccagatcga
     3841 cgcaggcttc gagtgcccgg gcggcaagag ctgtcggcgc ctctgcggca acggaacgct
     3901 gaacagcccg gaagcttgtg atgacgggaa cgagcgctcg gatgacggct gtagcaactg
     3961 ccagctcgac gcgggcttcg agtgcctgca gccgggtaag ccctgcacgc tactgtgcgg
     4021 caacggccac atcgaggtcc cggaggagtg tgacgacggc aacgacctcc cgagcgacgg
     4081 ctgcaccaat tgcaagaagg atccttgacc gcacgccgat ccagcgaatg ctggcttgaa
     4141 cggctggata gaggaacagg agcgagagat gccgagctac cgtttcgaac gtgaggtcac
     4201 ctgggctggt ctcgccacct tgttcctctt tcagctagtt gcttgcggcg agacgtccga
     4261 tcccaatcgc ggatcggact gcagcggcgc tgcctgcgcc gggcgcggtg gtgggtacac
     4321 gggaggccgt tcgatggccc actcgggagc cggaaactcc ggcgaaggct ccggcggcgc
     4381 cggaggaagc tccggcgaga ccgaggccag ttccggcggc gcacccgtcg tgagctccgg
     4441 cggagagaag aatacgggcg agggcggagc cgagggtggg gcgcgtccgg agggcggcac
     4501 gagccacggt tcggatccgg ggctttgcgg tgacgggaag cgcacgactg gcgaagagtg
     4561 cgacgacggc aacagccaag atgacgacga ttgcacgaac cagtgtaagc gcgccctctg
     4621 cggagacggc gtcactcaaa ctcgcgcctc gtctccagaa gcgtgcgacg acggagcgga
     4681 gagtgccact tgcaacgaag attgtagtga agcacgttgc ggggatggca tcgtcaacgc
     4741 gagcgccaag gaagcgtgcg acgacgggca agacaccgat cgatgcaata ccgactgcac
     4801 gccggcgaag tgtggggacg gttacacgaa tcgagccgct ggcgaagctt gtgacgatgg
     4861 ccgcgaaacc gagcaatgca acgctgactg caccatctcc tattgtcacg atggccgggt
     4921 gaatgccacc gacggcgaag agtgcgatcc cggtgacgac gacgagaccg acgagtgcga
     4981 cgcggattgc tccgaagccc gctgcgggga cgcccacgtg aatcaagctg ctggcgagga
     5041 gtgcgacgtc gctggcatca gcgcaacctg cgacggcgat tgcactcgtc cggtctgtgg
     5101 cgatggttat cccaacttcg ccgtagagca atgcgacgac ggcaacacgc ggaacggcga
     5161 cggttgttcg agcgcctgca agctcgaata gcccagaacc cgctgtcgtc gcctgaattt
     5221 tgcgtgagtg taggcttcag aacagagctt ccccgggcaa ctccgaggct tgctcgagcc
     5281 aactcactag cagcgattca tccagctcct cagcttcgtg aatgtgcaga tatcggacat
     5341 gcttttgttt ggactcgacg ggcggcaagg gctcgagcgc cgctccccgg aaaaacgtga
     5401 ccttcatgta tcgagtgaag cagtggaagg acacgaacca acctttccct tccagcccgt
     5461 agaatggagt attccacctg accgccttcc gaactcccgg cacggtgcgc acgatgagag
     5521 cgtccaaata tctagctaca tcgcgtttcc agtccgggat agccgcgatg taggcctgta
     5581 ccggagcgtc cccgtcggcc ttggagatct gcgggttgtc gccggaaagc agcctggctt
     5641 tctgcgcgga agcgcgaccg gcggccgcct tctgcgtggg ctgcctcttc ttcaccgtgg
     5701 ccttctttcg tgtgaccgtg gtccctttca cgttgccctt accctgacgc gcgccggcct
     5761 gggccgccag cgcggccgct gcggcgagct tgtgtttgct ctgccaggga gggcctaggg
     5821 ttcgacccaa gtcgtgcaag aaggtctcta aatccgcgtt gtccacctgc agcatatcca
     5881 cccgcagcgt ggcttcgctg acaacagact cgacttcgta ccaatgcacg cgccaccccc
     5941 gcgactcggc cgcggccgcg atcgcctgac gatacatgac cgagtcggca tatgtctgag
     6001 cacgattgtc cctgattcgc tgctcgatgg tcgcaggcaa cgcggggcac actcgaatcg
     6061 cgatggccgt aatcggcacc tcgaccgcct cactcagttc ctggagacgc tgacgcgcgc
     6121 ctttgcctgc ggcttcttgc acgcgctcga ccaacttcac ggcctccgga agcgagatct
     6181 cacgggccca aggggtgttc aggtatcggc cgaccgccca tgaaccttcg tggtgatggg
     6241 gatgcgtcgg tagcccagat tcggtcagat cgacagtgcg tcggtcgagc acctcgccag
     6301 atgcggccag agtcacgaga accgctgaat tgccgttctc cgccacgccc acactcgcga
     6361 actgctctgc tgcgcgttgg accatgcacc ctcctatgtc gaactcgggt gtctctcaac
     6421 cgaattccag cgccgtgcat cccagccccg cccagtcttg ggtccgccaa accggcccct
     6481 accagaaccg ggctcgacgt acgtcacgtc gagcccggcc cccacccgcc tcaccacccc
     6541 atgcaggtca aaagctcagt cacgattggc tcgcacgcgg gcccgatggc agagcctccg
     6601 tagccttcgg agcacgacca aagcgctgga tcgttcacgt cctgatctgc catgcagttg
     6661 ttggcgccga tgtagctctc catgcactcc ggcggaatga agccttcgac atctgcacag
     6721 ttgaagaaac attgcttcag cgtgctccga gtctcgatcg gcgggtcgca cgccgcggca
     6781 atcgaggctc ggcagctacg caggcagctg gatccaaggc tatctcgcgc acaacgaggg
     6841 gagctactac tggcccgcaa ttctgtggtg gggctattgg tagggctcaa cttccaatag
     6901 aagggctgac cattcgtgaa gtcgacgaag aagttcaaag ggtgatcgcc cgtcaagaac
     6961 gagctcggtc gtacgccctc ggtgtcgagc ggcaggtaat tgcgatcttg gctgtagggc
     7021 acctggatcg tgattgcgtt atcgttgcgg tacgagagca gcgccgtgta cgttccgtcg
     7081 gcgttgtctt ccacacactc gaggcgcggc actacaggag cttggcccga tttgaaatag
     7141 gcatcgtcca agaagggcag gaacgactcg agccgggtga agatcgaata gtcttcgcac
     7201 caaagccccg tccacatcga aacgcctgcg acgtagtctt gaccccattt attgataatc
     7261 ataggtccgc ccgagtcacc ggcgcaggtg ttcgctttgg gcgcgaggcc gctcgtcttg
     7321 atccaatgcg accgaatcgc cgcatccaac atgccaggat tcgggtcgtc ttcatggtag
     7381 tccgcggcgg tgggcgtgcc cagaatcggc acctgcgcgc tccgcttcac gccgccaccc
     7441 tccacctgct tgatgtccgc gctcagggcc ttatttgcgc cccagccgac cagcgtgccc
     7501 ttcttgccta tccatgaatc atcgaggcgc gcccgataga tcgggagggg atcgatgggc
     7561 ggcttgcgat ccaagtagag taccgccaga tcgggatagt gagtgttagc gtcgtagctg
     7621 gggaacgcct cgtccgcctc cgccatcgcc caaacgtttt gctcggattg gggcccgatc
     7681 gtcgacggac cgatggggcg cttgttcagg aggctcagat cgctcgcgta gtccttgccg
     7741 tagtaaacga aggtgccgtg ccaccaacgt ccgtttcgca gacaatgagc ggccgtcagc
     7801 actacgcgcg gagcgaccaa cgtacccgta catcgggtaa cgctcccgcc gtaaatctcc
     7861 gagtggatca tcaccacgta agggcggttg ggctcattgt tcccccgcac gatctcgctc
     7921 tgttttgcgt ccacctgctc agcgtcaccg gaatacgcgc tacagctcac gccgaacacc
     7981 aagctcaaca gcaaaaactt tcgtctcatg gcgtcactcc tgtgatccgg gcggattcac
     8041 gagcgaagac tctgatattc gcccgacccc cgcaactact catctgacag ttgcttctcc
     8101 caaagagcaa atgacatgtg gaacatctaa tttgatgcgg gaggcggcgg cgcatcctcg
     8161 acgggaccag ccggaacgta agcgaatccc aatccgagcg atcaggccta agcgggcctt
     8221 tccgtgggcc aacctgtgcc agcttgagtc agcccgattg cgacgcagcc ggggtcgcaa
     8281 tcctccgtaa cgaccgcggt gattgggcgt gacgagactt gcgagagcgg cggttgcctg
     8341 gccgtcagcg atgaggcggg cgtcggctcg tgcacggtcg cttgcaccac tgacgcggat
     8401 tgctcacccg gtgctcagtg ccgcgaagtc atcgatcagc gatacacctc gtggcgccgt
     8461 ttggattaca tcgtcgagct cccgcgcatc ctggcttgtg tacccgagtg actctgcggc
     8521 cgtgcgatct tacgtcgccg aagggccaat agcagagctc ccgccccgaa gagtgccgag
     8581 gcaccgagcc gcgcgccgcc tccggcgaca ccgcagctac agcccgattc gtgctgaaat
     8641 cgatttgctg ggtagatggc gcaaataccg gcaatgtcgt ccggctccag cgaacgaggc
     8701 cccaagtcac caccacccgc ataccgacca ttcatggtgg ggcccaagcg aaatagatcg
     8761 gccaagccga agacatggcc ggattcgtga gtcagcacgc tcagcaagtc gaagtctacg
     8821 ttcgaatccg aagccgtgat atccacttga tccgagttca cttcgatgtc agtggccacg
     8881 atacgcccgc tcttcacccc aaacgtcact gtcgtgagcg ccacgacatc tggcgagtca
     8941 tatatccagc cctcatcgac gaaggccaga acattgttac tcacgtcctc ttgcgtcgga
     9001 tgatcctcgt ccgggctcgt caggccttcg cgaaccgcaa ttggatctcg gaccaagtct
     9061 accgaggcga cgctgatgct ggggtgcgtg ccgtcctcgc agaagaccga agtccaagcg
     9121 gtaagggact caatcaagac ggcttgcgcg gcttcccccg agatgttccg tcgcggggac
     9181 ccgctcgcgt cgaccaaaaa gcgtatcgac tcgcccggcc agaacagggg agcacccacg
     9241 gtgggacact tctcggcatc tggcatacac gcgggagcat ccgagcgacc gcaggtgcgc
     9301 gttcggcagt aagcctgagc actgctggtg ccaatcgctt gaacgacggg cgcaacgacc
     9361 gcgagcgcgc caaaccatcg aagcagacgc cccaaaaccg acatatgcgc aaggtatcac
     9421 acggccaggc ctcgctcaaa tcgagcggat caagtctcta gatcgatcga cgcaatcagt
     9481 tcgcccgtca ctattcctcg ggcgttcgtt acggctgggc gtactcgttc ggagagctgc
     9541 gggagtcgat cggcagagag gccgagcctc cggagcacgg ccatgaccgc gggcgccagc
     9601 gcgcgctccg cgccgtccaa cactttgacc gcgatgccga gcggcgggtc ttcgaaaccg
     9661 atgagctgaa tcgcctccgc gcctgccttg caaaccaagt tatccgggaa cgcacacgtc
     9721 aactggtagt cgaagcgccc tggtcccgac acgagtagag gatgcgtcaa catcgcccta
     9781 cgaaccgcgg ctagccccgc acaactctcc gaagcatccg cggaagcaag tcgtttcgcc
     9841 gcttgggcaa gcacccggag cggcagcgcg tagttcggag cgctgcaacc atctactccc
     9901 gagccaagca ggttctcatc gatttcacag gcactcgcga gcacggcgcg gaccagagtc
     9961 tgcaccttgg actccggctc tagataagcg gccaacggct gttcgagcgc gcgggcaacc
    10021 gccagaaatc cggcatgttt gccggagcag ttgtggcgca gcgggtccga gtcctccccc
    10081 tcgctcggat atctattttc gagacgcatc ccgatcggcc attgcacacc acatccgagg
    10141 tctcccgcgc ttgcaccgat cttgccgagc aacccttgaa ccaccgccac atgtcgatcg
    10201 ctcccgctat gcgaagcgca ggcgatcgcg agctcttcct ggctgagctc gaactgccga
    10261 gccgcgccgg acggaaagag cgcgagcgcc tgaaaaggct tcagtgaaga gcgtgcgaag
    10321 actacggcct ccgggtcacc cgcgcgaagc aagtgtccac gttcatcaat caccgcgacg
    10381 gcaacctcgt gaaccgcgtc caagccgtct ccccgcgtca cgtacacggt gctctttcgc
    10441 gtgaaatgca tggtgcgatc ttaacgcgtt ttcccggtgc ggcactcgtc gccccaacca
    10501 acccgggttc caggctcgtg gcgctcgaag ggcggctgcg tgcactagat gactcacgtc
    10561 agccgcgcga agactaacgt aacggcacac ccccgggcgg agcctacggc ttgcgacgtt
    10621 cgctcggttt tgcgagtatc ccggtgaggt tcaggcatgc gggtttacaa gaccagagag
    10681 ttacgttcga aaacttgggc agtcggcatg ctcgcgactg ccgctctcgt tgggtgcggg
    10741 agcgacgaag accaaaaccc ggccgcagca ggcggccaaa cagcaggcgg ccaaacagca
    10801 ggtggcaccg acagctcaat cggcaacagc gctggcggca gttcgaacgt cacccccgcg
    10861 ggctcggcgc caactttcgc tgcgaactcg gaggtcaccg cacccgtggt ttggggcacg
    10921 gaagacgcga aggtgcggat taatcatgtc tccaatttga acgctggaac gacgttcgca
    10981 ctcatcgctt ccgtggacgg catcccgtgc agcaagcttg ggctggccga cgctgattcg
    11041 accattctcg agctagcctg caccattccc ccgggcaacg ccgacaaagc accgcccaag
    11101 ctcttgattc agcaactcgg ctacacacaa aatctaaaga ctgtatatct agaagtgcct
    11161 cggggttcct tcacgctgcc ggtcgaggag tgtgaagcca gccctactcc cgccgagggc
    11221 gtggctctcc cgcagctttg cagagccgat ccaggatcga tcgcggccac gggccgcggc
    11281 ttcgagggca tttacgcagg cacggtgccg cacgtatccg atacgcaatc gcacaatttc
    11341 ggcttcgtcg ccggggatgg cacctacgtc tccgggaaca atacgctatg gcaagtggaa
    11401 ggcgcggggt ggctcgaggc gggccgcatg gtgcccaatc aggagccgtg gagcccgctg
    11461 caaggcttca gcttcgacat cgccgagggc atctccgtca aatcgggcga gttctaccca
    11521 gccgacgcca ctggcacatt tatcccatat accggaaatt cggttttggt gaacacgtac
    11581 tccgtcgcga acgcactcgc agtatcgatg tcgagcgtgg ccggagtctg gaagaacaag
    11641 gactccggat tgtccctctc cattgcagtc gaaggcgatg agggccaaat cacgggtagc
    11701 gtgtacctga cccccgaact tcagacctgc gcaatcagcg gaacgattcg acttcgagag
    11761 ccggatacgg caaagaatct ctacgacatc gcgctcgagg taacgcaggt cgagaattcg
    11821 gcaaacatcg agtgtgacac ggcggaccac gcgaaactcc ggggtctcgc cgccatcgac
    11881 ttccgcaacg tgggtacaag gctctcaccc gtgtacgcgc acacgctgga cttggccgcc
    11941 tacgaggtgg ataaagccat cttcagcgat accctcgcca aagagtgaga cctcatagcg
    12001 cctagcacgc aagcacccgc ggccaccgaa agcgcgcggg tgctcctcca gcatccctca
    12061 gaacttgaag ctcttcttca gagtgacggc ggggcccgtg aaggcaggga cccgcacgtc
    12121 tttgaggagc ttgcccacac agttacgcgc ggaggcccgg gcgaagcgga tgccacccag
    12181 gttcgcagcc gtgaccttac ccgcgggcgc gaacgtgatg ctgacttgaa gctgacccga
    12241 aggatcaccc ggatgcttac agctcgcggc gtgctccatg gtcgccttga gcgcttcact
    12301 tgccgccccc tcgtcgaagg cgcccgcctc gccttcgggc tttgccggtg ccgcaaccgc
    12361 cttgccctct gctccgtctg gagctgcgtt gctcggggcc ggggccgccg aagctgcggc
    12421 gggagcggcc gggctggccg cagcagacgc cgcgctcggt ggcgaggcag aagcgggttg
    12481 cgcgggcggc ttggcctcgc ccgcgggctc cgacacggca gcatgcttcg tcaccgtggg
    12541 cacggctgaa ctgggagcag tcgcgcgtcc gatgatgaaa gccataacga ccgcgccgac
    12601 tccaatcaac agcttcgaat gcttattcca aacgctcgct tcgggttgat aagagtgcca
    12661 actacgcccc gctacgggcg ccgcggacac cacctctccg ggaggaggcg gagttccgag
    12721 caacgccgag acaatcgagt tcgggggata gtcgccgtgc gtactcggcg tgcccatcga
    12781 tcccgtcgat ccgatggaac cgttcattcc gtgggagccg ttgactccgt tcgagccatt
    12841 catgccgtgc gcgccgctgg cgctgagctt cgcctccgca agaccagtcc ccgggggcac
    12901 gacgatattc gaagctccag acttcgatgc tccccaagga gtgttggttt ttggcaacgc
    12961 cgcctccggc tcagccgtgg tttcgtccgg ttcatcgcgg agcgtctccg acgcggcggg
    13021 ctcggaagcg gcggtgggct tggctacggc ttgaggcaca ctagccaccg ctgagctgga
    13081 cgcggcaggc cgttctgccg cgctcaacag ctgatccacg cgctcgagtt gcgccgccaa
    13141 cgccgcgctc aaacccgaac tgggattcgg gcgagtggcg gcagggatca ccggaggcgg
    13201 tgggggcggc actggcagcc ctacgtttgg cggaggtcct gcgcttgcat ccggaaccgt
    13261 cccctctgct gcattcgcac ttggcgaccc ggtccccgca ccggccaact ccgcgaccgc
    13321 agcagcaacc aaactcggct tcgtggacga cggctccgcg gccacgggtt cactcgcgac
    13381 cggagtcgtg acgcccgcct ccaccgaact agctgcgctt gctgggctcg cagcggccgg
    13441 gacctgggcc gcggcgcttg ctgcgttcgc ttccggcttt ggtgccacca acgtgagtgg
    13501 tttgatcgga gtggcacgtt tggccggtac cacggagcgc gcactgttcc ctcgctccgc
    13561 gctgccccgc tcattgggtc gcggtggttc actcgggcgc ggcgcctcgc gcgactcgga
    13621 cctctcatct gcacctttga gcttcgtcgc cgcaaaaggt tgtgccggag tcttcagctc
    13681 ggagagcccg accggagccg acgagacgac gctcgccttc gactcgagcc cgcgagcctc
    13741 cccgacgcgc gcccccgaat ccagcttgga agtgccgagc gacggactgc gcgtcgagct
    13801 gggcaccgtt gaagcgggca ccgtcgaagc gggcaccgtc gaagtggaca gggtagaact
    13861 agacacggtg gagtccggca gggctgaact cggcagggcc gaactcagcg gcggggttgc
    13921 cactggaaca tccgcgggcg gggcggccga ctccggagtg gacttgggcg atggcgaaga
    13981 ttcgttattt gcgtctgctg aagtcatgag gctggccacc gggtgaggtg caagggcgtg
    14041 agaatcgagc acagtagatt cgagaacggg agagacagag gatgcagaga ccgcgccggg
    14101 agacccctgc ggagatagga ttcgaaccgg catctccacg tggtcttccc acccatccgg
    14161 ctccatcaac atcgcgttga ggctgtttgc ggagggcatc ggcaagcgcg gatggagccc
    14221 gcgattgcga ttgggttcag aaaccgactc ggcgtcgggc gccgcgacga tgtcgccctc
    14281 gctcacaatc caagccgcat tcgaatccgg accagcagcc tcgagcggct cccgagccgc
    14341 agcatcgctt agctcgcgcc gactcaccag cttgctgtcc gaagcctctt catcctgcga
    14401 gagctgcgga tgcaccgcgg gatccgagat atcctctcgg acgtcgcgct cgacacggtc
    14461 ttgactggtc atgcgatcct ccacgatggt gcggtcactt ctccgggcgg tagggtctgt
    14521 ggcggtactc cgccgctatc ctacctcatt cgggaagcag gcaaactttc ccaacgctcg
    14581 atcccgcccc gagccgccac tccatgggat tgaactgaat ttgcactctt ctcgtggatc
    14641 gccgaaccag ccaaatcgca ttcacgcttc ttcggaaact tccatcgaac acgcgagtga
    14701 ttcggcctcg tctcgcactc accatcggct ttccgggtga tgcgaaccag atggatacac
    14761 aaagccacca aatgagccgc gtgattcgat gagcgcgcgg gacctgctga ctttacacct
    14821 ctatccggcc gcaccttcag catcggaggg gccaacgccg cccccccttc cgtactccag
    14881 ctggtgaaca gctccacagg tgtgcagccc cgcaggtgta gagccccgta cgttcatcct
    14941 gcgactctag agatcgctcg ctcaacggcc ttgcggcgca tcgttgaccg aagggctgct
    15001 cggccccagg ttcctggttc acgcgatgac gaaatcttcc ctgctcagcg tagtcccgct
    15061 cggattccca tgggccacgc tcgatccctt cctgttctgc gtccaccacg acgaccacta
    15121 cccgcgggga aacgagaaac tcggccctga cgcttcgctc gcggggcgcc gattggggca
    15181 ggacttcgaa atcaaagacg gctttcgcat gtatcacggc cgcgaagtgc cgggcttccc
    15241 tcagcacccg caccgtggct tcgaaaccgt caccctcgta cgccgcggct tcatcgacca
    15301 ttcggactcg ctcggggcca ccgcacgctt tggcgctggg gacgcgcaat ggctcactgc
    15361 cggccaaggc atcgtacatt ccgagatgtt cccgctcctc gagcgcgatc gcgacaaccc
    15421 cctcgagctc tttcagattt ggctcaatct gcgggcccaa gacaagctcg tcaaaccctt
    15481 cttctccatg ctctggcgcg agaacatccc tgccctcagc gtgcccgacg aagaaggccg
    15541 aaccacccag ctctacttgg tcgcaggcga atacagtggc cagcgcccgc cggcaccacc
    15601 accgaattca tgggcgagcc agcccgactc cgacctcgcc atttggacgt tgaaactcgc
    15661 gccccacgcc cgcttcacac taccgcccgc gcgcagcggc acccagcgcg tactctactt
    15721 cttcaagggt gacgaactca ccatcgacgg cgaaaccgtt cccgcccaac atgccgtcca
    15781 agtggctgct gacaccgaat ctcgactcca gtgtggcgcg caccccgccg aactcctctt
    15841 gttacaaggc cgaccgatcg gagagcccgt cgcccaacac ggccccttcg tcatgaacag
    15901 ccgatccgag ctccagcaag cctacctcga ctaccagcgc accggctttg gcggctggcc
    15961 cttccccgaa gacgcccccg tacactcccg cgaagcgggc cgcttcgccc gccacgccga
    16021 cggccgcatc gaacacatgc cctaagcgtt gacttcgagt cttggtattg gccacctcgt
    16081 aaagagcgcg acactcggca ccatccccgc gagcgcagcc cctgcccccg cgtactcccc
    16141 ccgcaccgcg cctgccccca caacaacctc ctcgcgtcac gccccaacgc caactccggc
    16201 gtccttgcaa actcccgcac cgcactttgc cgaatcaaaa ccacttcgcg ggccctccaa
    16261 cgaacacgcg cccgcaccga cggcgcccac gggaacatcg acgacactcg cgggcacatt
    16321 ttcgacactc gcgggaacat cgacgatact cgcgggaatt gcgacgcacc tcgcgggcac
    16381 attttcgaca ctcgcgggaa catcgacgac actcgcggaa attgcgacgc accacgcggg
    16441 caacccacga cactcgtggg aacatcgata gcccacgcgg ggttgagtgc gttgcggcgg
    16501 ccacgtcgcg gccgcgacgg cccgcgttcc catgctcgcg cggagcggtc acaacgccaa
    16561 ctcgcgaacg gctcggcggc agcattgccc acgcaggcgt gcaagtccta gcgcgcctt
//
LOCUS       DASTKU010000471         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1429538, whole genome shotgun sequence.
ACCESSION   DASTKU010000471 DASTKU010000000
VERSION     DASTKU010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429538"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFQ61_16375"
     CDS             complement(<1..372)
                     /locus_tag="VFQ61_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956085.1"
                     /translation="MKVLIVDCQTTGAGPAFGRVLEIGWGVACAGSPEPQFAQAHWVT
                     LPEGHVVPSQVRKLTGYDANASRSEISESEAWRRLRNALSSTETGSLDSAGTLVPGAS
                     LPTAIHFARFELPFLRDWSQRF"
     gene            550..1419
                     /locus_tag="VFQ61_16380"
     CDS             550..1419
                     /locus_tag="VFQ61_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956086.1"
                     /translation="MQIKVIGTLSWPDISARRPFAELTFHVLAHVPNPTPGSLYDPAY
                     IARVATWLGAASDRTLGEDVRLLVEGTRDFAGYARIQNLAWLFATPEQAQKVAAVDIT
                     ALTPTQVEDPSALAALCAPAWQGLKNTIELLRCAITLESAVFQRLPSWNWSRDALRDW
                     LRRVRSAAPSLDQFRISTLRSLGWRGRVRGREIWIGEPFESGPSVAHLAFQAAHEASV
                     AELNDLDCSERERESAALVLFAQRARAAGLAQEHAKWLQHFGGTIGTDPVALRRELRD
                     AVEQRLRTASDFD"
     gene            complement(1405..>2674)
                     /locus_tag="VFQ61_16385"
     CDS             complement(1405..>2674)
                     /locus_tag="VFQ61_16385"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956087.1"
                     /translation="GALEGYLDKMISVSEWARECDVPFGANIIVGHPGETEQSLRTSA
                     AYMRRVFTEPAAGTHGFLSVDPFRLYPGSPIDRERERWESETGMVVHRYPWWHDGDQA
                     FLSEWVDPSAELDYRRTRSLQDQLFTPILSEIRARFGYSGPARDYFLRAIDQQIADAQ
                     PRARLGTLGLHRLWQALTSDSSAKNARALLAEDSELRSVARAARSELARGLTSRDDLR
                     RALEVVPRERFVPLDRVGESADDVALPLTEDGRSSISALHAYVNAIEWLSIQAGQRVV
                     EFGAGAGYGAALIAEIVGPTGCVLAIESQAELLPLLTENLAAYPRVFVEHGFGRAELV
                     PGAQRVLVSFALSSVPIEWLRALDEGAMLLAPVGAGTQRMLRYTKTAGKIECAFMGEV
                     VYVENRDVSPLASVATARGEERVDGRSIEV"
BASE COUNT          490 a          888 c          815 g          481 t
ORIGIN      
        1 gaagcgttgc gaccaatctc gcaagaacgg caactcgaag cgagcgaagt gaatggcggt
       61 tggaaggctc gcgccgggaa ccagcgtgcc ggcagaatca agcgaaccag tctcggtcga
      121 gctgagcgcg ttgcgcagcc gccgccatgc ctcggactcc gagatttccg aacgcgaagc
      181 gttggcgtcg tagccggtca gcttgcgcac ttgacttggc accacatgcc cctcgggcaa
      241 tgtgacccaa tgtgcttggg caaactgggg ttcaggtgag cccgcgcacg ccacgcccca
      301 gccaatctcg agcacgcgcc cgaacgcagg tccggcgccc gtggtttggc aatccacgat
      361 tagtaccttc aactcagcaa gcgcgcgcac cgcacaagca atgcgaacgc gctgagcgcc
      421 cgtcaagtgc cccgtgctat gtgcctcact gctagcgcta ggcggtacac ggtcccgagc
      481 ggcaattcgc acgtgccgcc gcgttcagcc atcgagctgg catgcttgcg tcgggctggc
      541 atgcctggca tgcagattaa agtgatcggc accctgagtt ggcccgatat ttccgcgcgt
      601 cggccctttg ccgagctcac gttccatgta ctcgcgcacg tccctaaccc cacgccaggt
      661 tcgctctatg acccggccta catcgcccgg gtagcaacct ggttgggcgc cgccagcgac
      721 cggactctcg gtgaagatgt ccgtttactc gtggagggaa cgagggattt tgccggttac
      781 gctcgaatcc aaaaccttgc ctggctattc gcaactcccg agcaggcgca aaaggtcgcc
      841 gctgtcgata taacggcatt gactccgact caggtcgagg acccctccgc acttgcggct
      901 ttgtgtgctc ccgcctggca agggctgaaa aacacgattg agttgctccg ctgcgcgatt
      961 acgctcgaga gcgccgtttt ccagcggctg ccgagttgga attggtcccg cgatgcttta
     1021 cgtgactggc tgcgccgggt gcgatccgcc gcgccgtcac tcgaccagtt tcggatttcc
     1081 acacttcgtt ctctgggttg gaggggccgc gtgcgcgggc gcgagatttg gattggtgaa
     1141 cccttcgaaa gtgggccctc cgtggctcac ctggcgtttc aagccgcgca cgaagcgagc
     1201 gtcgccgagc tgaacgactt ggactgcagt gaacgtgaaa gggagagtgc ggcgctcgtt
     1261 ctattcgcgc aaagggcgcg cgccgccgga ctggcgcagg agcacgcgaa gtggctgcag
     1321 cacttcgggg gcaccatcgg gaccgacccc gtggcacttc ggcgcgagct gcgcgatgcc
     1381 gtcgagcaac ggctcaggac tgcttcagac ttcgattgag cggccgtcga cgcgctcttc
     1441 cccccgagcc gtcgcgacgg aggccagcgg cgagacatcg cgattctcga cgtacaccac
     1501 ttcgcccatg aaggcgcact cgatcttccc cgccgtcttc gtatagcgca gcattcgctg
     1561 ggttcctgct ccaaccggcg ccaacagcat ggccccctca tccaaggccc gaagccactc
     1621 gatcggtacg ctcgacaatg cgaagctcac gagcacacgc tgcgcgcccg gcacgagctc
     1681 agcccggcca aatccgtgct cgacgaacac tcgggggtaa gcagcaaggt tttccgttag
     1741 tagcggcaaa agctccgctt ggctctcgat cgcgagcacg catcccgtgg gtccgacgat
     1801 ctccgcgatc agcgcggcgc cgtaaccggc gcccgccccg aactcgacca cccgctggcc
     1861 cgcctggatc gatagccatt cgatggcgtt cacgtaagcg tggagcgcgc taatgctgga
     1921 ccgcccgtct tcggtgagcg gcagcgcgac gtcatctgcg ctctctccca cccggtccag
     1981 cggcacgaac cgttcacggg gcacgacttc aagcgcacgc cgtaagtcat cccgagaggt
     2041 caacccacgc gcgagttcgg agcgagccgc acgcgcaacg ctgcgcagtt ctgaatcttc
     2101 agccagcaac gcccgcgcat tcttggcgga agaatcgctc gtcaaagcct gccagagccg
     2161 atgaagccct agggtcccga gccgggctct cggctgcgcg tcggcaatct gctgatcgat
     2221 ggctcgcaag aagtagtctc gagccggacc gctatagccg aagcgcgcgc ggatctcgct
     2281 caaaattggc gtgaacaatt ggtcttgtag gctgcgggtc cgccggtaat cgagctccgc
     2341 ggagggatct acccattcgg acaagaaagc ttggtcaccg tcatgccacc aggggtagcg
     2401 atgcacgacc atccccgttt ccgactccca acgctcgcgt tctcgatcga ttggcgagcc
     2461 cggatatagc cggaacggat cgaccgacag gaagccgtgg gtgcctgcgg ccggttcggt
     2521 gaacaccctg cgcatatagg cggcgctcgt ccgcaagctc tgctccgtct cccctggatg
     2581 tccgacgatg atgttcgcgc cgaatggcac gtcgcattcg cgtgcccatt ccgagaccga
     2641 gatcatcttg tcgagatagc cttcgagtgc gccc
//
LOCUS       DASTKU010000472         3599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_758238, whole genome shotgun sequence.
ACCESSION   DASTKU010000472 DASTKU010000000
VERSION     DASTKU010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758238"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..752
                     /locus_tag="VFQ61_16390"
     CDS             216..752
                     /locus_tag="VFQ61_16390"
                     /inference="COORDINATES: protein motif:HMM:NF028012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HET9956088.1"
                     /translation="MRPPGYSHPALTEFAAIAGAFCAAVANGVSRKPAEQLDQIHRIL
                     PRVYSAALQLPDVSTVFANDDERELEAAQPVVVPPGLAQLAEFLGERRFYREVYDPYA
                     EPTEGECTGDLIDDLGDIYQDLRGGLVHWENGEPREALWAWRFNFQIHWAEHATSALR
                     ALFALSAWRDVPWPSGTR"
     gene            1042..1275
                     /locus_tag="VFQ61_16395"
     CDS             1042..1275
                     /locus_tag="VFQ61_16395"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956089.1"
                     /translation="MRQQDAEQIQHAIRHGLTAAVQVRLNDPTYGAPTRSRLTTPEAR
                     TAVKACVSSAFRDFLQKEASALAFFTARIARDD"
     gene            complement(1659..1958)
                     /locus_tag="VFQ61_16400"
     CDS             complement(1659..1958)
                     /locus_tag="VFQ61_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956090.1"
                     /translation="MRLNLLPELGALPIEYLDVDRIERFKAERLAAGVHRKTLNNLLV
                     VVRAVLQLALERREIAAVLIVRGLPVAARLCIAPPRWLATAHDMARDSASARPRH"
     gene            complement(3023..>3599)
                     /locus_tag="VFQ61_16405"
     CDS             complement(3023..>3599)
                     /locus_tag="VFQ61_16405"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9956091.1"
                     /translation="LMECVSLRVRDLEFALGQLAVRRGKGQVYRVTLLPVAIREPLRL
                     HLRGVRALHQRDLARGAGAVELPNALGVRYPNAAREWAWQWVFPASRQYEHLETKSLR
                     RHHVRETVLQRAVREAIQRSGIAKAAVCHTFQHSFATHSLEAGYDIRTIQKLLGHRDV
                     RATMIYTHVVNRGPLGVRSPLDDARFGRNQT"
BASE COUNT          681 a         1108 c         1160 g          650 t
ORIGIN      
        1 gtccgcgctt ctcgacttct ccagttgcca cacggatgtc ctcgttttcc cggtcgcctt
       61 gagctggact atggctttca cccacgagca accggagctc ggcccgtact tctcacgggc
      121 tgaatggtgt gagctcgacc cgagcagcaa ttaggaaacc gaggtcgccg cgcgcatagc
      181 gcgagcagtg gtctaattac ggttaacata acgcgatgcg tcctcctgga tactctcatc
      241 ccgcactcac cgagttcgcc gcgatcgcgg gtgccttctg cgccgccgtc gcaaatggcg
      301 tttcccggaa accagcggag cagctcgacc agattcaccg catcttgccg cgggtgtact
      361 cggccgccct gcagttgccc gatgtatcaa ccgtgttcgc gaacgacgac gaacgcgaac
      421 tcgaagccgc ccagcctgtg gtcgtaccgc cgggattggc acagttggct gaatttctcg
      481 gggagcgccg cttctaccgg gaagtctatg atccttacgc ggaacccacc gaaggcgagt
      541 gcacgggcga cctcatcgac gatctgggcg acatttacca ggatcttcgc ggtggcctgg
      601 tgcactggga gaacggcgag ccacgtgaag ctctctgggc ctggcgcttc aattttcaga
      661 ttcactgggc cgaacacgcc acgtccgcct tgagagcact ctttgcttta tcggcttggc
      721 gcgacgtgcc gtggccaagt ggaacccgat agcaggaagg gactattgga tgtcgaattc
      781 tcgccatcac gccaccaacc cagagttgca ctggacgaaa tgagcatcga ccggattgat
      841 tgttagaatc tgccgaatta gataaacttg cgcgtctcgt ggcccgccgc tttggattca
      901 gatgaacgca gcactgattg aacgattgcg ggcgtggatc cgcgctggcg cgaagccgga
      961 cacgttgtcc gacggaggga ctcatgttcg cggcttactg ctggggctcg ccctcgggct
     1021 gcagcaagcg gctccgcagg tgtgcgccag caagacgccg aacagattca acacgcgatt
     1081 cgtcacgggc tcaccgcggc ggttcaggtg cgcctaaacg atcctacgta cggcgcgccg
     1141 accaggagcc ggttgaccac ccctgaagca cgcaccgccg tgaaggcgtg tgttagcagt
     1201 gcatttcgag acttccttca gaaggaagcg agcgcgctgg ccttcttcac ggcgcgcatt
     1261 gcaagggacg attgaccttg cgattttgcg tttgtggtca ccgaagctcg gccgacggca
     1321 actcaagaga tcttaggcta cgcaccaccg cgtggtggag tctccgtctg cccggctaac
     1381 tctccccata gagactaacc gggaagcgcg cgtcccttcg gtttcctgat gcgctcgtaa
     1441 ctcaccagcg tcaggccgcg gaacgagggg gcaataccta aggcccgagc agttggccgg
     1501 agagggcctg caacaggggg cgtgtgagcg ggaactcccg cgcgccgacg cggagggtgg
     1561 ctcggtcgag ctggacatca ctccaacgaa ggtttcggag ctcccgcagg cgaaggccgc
     1621 acacgacggc cagcagggcc atagtgtgaa agacgggttc agtgacgcgg acgagcgctc
     1681 gcactatcgc gggccatgtc gtgcgcggtg gcgagccatc gagggggagc gatgcacagg
     1741 cgcgctgcga cgggcagccc gcgcacgatc aggacggctg cgatctcccg ccgttccagg
     1801 gcgagctgca agacggcccg aaccacgacg agcaggttgt tgagcgtctt ccgatggacc
     1861 ccggccgcca agcgctcggc tttgaagcgc tcgattcggt ccacatccag atattcaatg
     1921 ggcagcgcgc ccaactccgg caagagattc agccgcaaga gtgcctcgga gcgcgagcgc
     1981 agacgcgagc tctgcaaagg tgctcatggc caccgccttt cctgcgacgc gacggtcgac
     2041 cccgggcaaa gccttcggcc agaccgtttt ggcggcctct ggctgcaaat tggagaatgg
     2101 acgcgaaaac gcgtgcatat cccttgtgtg tgcgcacgcg tacttgctag ctgattgggc
     2161 acccgtcccc cggatattgt gcgttcacgg tacgcattac tgcgtgagcg ggttcgcggc
     2221 tggccatccg gagaacaaga ggagaggcgt actgctcacg gagtggcttg aaatcagccg
     2281 cgaaagccga acggaccgaa aaaacgagtt caagggccat agccaatcag ctgccggctg
     2341 cagatcggtc tgataccgaa tctcagaatc cgcacccgcg agctcgagcg atcagaacct
     2401 caaatcatct ctggcaccca cgagcggact cggccgttgc ggagaatcga aaagggcagc
     2461 cgggagccca tttcgatagg gggtcgaccc gaaggtgacg gtcgacccgt cacttttcgg
     2521 tccgcggtga tggggggtga cggtcgaccc gtcacttttc ggtccgcggt gatgcggggg
     2581 tgacggtcga cccgtcactt ttcggtccgc ggtgatgcgg ggatagcggt cgacctgtcc
     2641 tgtttgggtc ggcgtcgatg gcaggtgacc gtcgatctgt cctgttcctc tcgaggtgaa
     2701 taaggcccaa gggggttgac tccttcggac tgggccggca cggacggaga ggggcggttg
     2761 aaccgtccga gctgggttgg cccgagcggt gagcgacggc gaacccctcc ggtttagatt
     2821 ggctccgacg ccggggcgac ggtggaagcg tcgaattcgg gtcggccagg gtgagtggcg
     2881 gccggtctcg gggtccgcgc gcgagcggcc ccgagaccgc ggtcctaagc acctgcccga
     2941 tgcggtgtcg caacacggca gccccacggt ccttagcgat cgacgtgagg ttagccgccg
     3001 atgtaaggag agactcggcg gactacgttt gattgcggcc gaagcgagca tcgtcgagcg
     3061 gactgcggac cccgagcggg ccccggttaa caacgtgcgt gtagatcatc gtagctcgca
     3121 cgtcacggtg cccgagcagt ttctggattg tgcggatgtc gtagcccgct tcgagcgaat
     3181 gcgttgcgaa agaatgctgg aacgtatggc agacggcggc cttggcgatc cccgagcgct
     3241 ggattgcctc gcggaccgca cgttgcaaca ccgtctcacg tacgtgatgt cgccggagcg
     3301 acttcgtctc cagatgctca tactgccggc ttgccgggaa gacccactgc caggcccact
     3361 cgcgcgcggc attggggtac ctcacgccga gcgcgtttgg cagctccacc gcgccggcac
     3421 cccgcgcgag atcgcgttga tgcagcgcgc ggactccccg cagatggagg cgtaaaggct
     3481 cgcgaatggc gacaggaagc agagtcaccc gatacacctg acccttaccg cgccgtaccg
     3541 ccagttgccc caatgcaaac tccagatctc ttacgcgcaa gctcacgcac tccatcagt
//
LOCUS       DASTKU010000473         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_312599, whole genome shotgun sequence.
ACCESSION   DASTKU010000473 DASTKU010000000
VERSION     DASTKU010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312599"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1936
                     /locus_tag="VFQ61_16410"
     CDS             <1..1936
                     /locus_tag="VFQ61_16410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HET9956092.1"
                     /translation="YDYDAAGRVTGVQHSDGSNERYAYRPDGELTEATNAATVVRFER
                     DLLGRISREQQGEHWVGSEYDSLGRRKTVRSSKGLMQRIRRNSMGDVVAIEAGVHTLT
                     TEGTDPSTLGRIFGERDPYRVDFERDRLGLELQRNLPGGVQARWQRDRLGRPELHEIR
                     VHGVVQSARRYEWDSNDRLQAILDALKGPVQYSHDALGNLAAATYADGRVEWRMPDAV
                     GNLFRTRERDDRKYGPAGQLLEARDARGVTTYAHDAEGNLIEKTEPDGARWEYRWNLS
                     GMLIEVRRPDGDVVRFAYDALGRRIWKAYRGKSTHWIWDGNVPLHEWITLNDEALARD
                     GAPIATSAELAIAIAKREALLARRSAQGPPEVPTLEGTADSPITWLFEPESFSPLAKL
                     VADERYSIVTDHLGTPRAMFDERGRQSWAAETDTYGRLSDVRGLRGACPFRFPGQYED
                     AETGLYYNRWRYYDPEGGGYVSRDPIGLAGGLASYRYTADPLFWFDVFGLSTSCGHHA
                     IEQTPISRRNARRLLRDRGLSNKRAAELVDSFEGQIYAVKGKAGEVFAITESNPGAAS
                     AVFVTRDFVAPSPTERASKLALPPSNAAEVEGLVELTRDQLLLEGRVAPQLQWGADKT
                     GGAWQTVTDGGRYTGAVRRI"
     gene            1940..2230
                     /locus_tag="VFQ61_16415"
     CDS             1940..2230
                     /locus_tag="VFQ61_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956093.1"
                     /translation="MASPRAAGQDVLDLLRDHGFFDDAARLERALSSLDSGAPQVRAA
                     AQREILNMCHPRWLGDLYIEGLSLTDWSGKLDALSNAVQGSHAQSKEARRRS"
     gene            complement(2399..2680)
                     /locus_tag="VFQ61_16420"
     CDS             complement(2399..2680)
                     /locus_tag="VFQ61_16420"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HET9956094.1"
                     /translation="MTRFLSNAKIPVSNNLSEGQLRIVALGRKNYMSVGAKSAGQSIA
                     TLYTLTACCKRARIDPIAYLTDVLRRLGRDPTCSVDELMPHRWKPLDSS"
BASE COUNT          574 a          832 c          972 g          539 t
ORIGIN      
        1 gtacgactac gatgccgcgg gccgcgtcac cggtgtccag cacagcgacg gatcgaacga
       61 acgctacgcc tatcgccctg acggcgagct cacggaagcc accaacgccg cgacggtggt
      121 gcgcttcgag cgcgacctgc tgggacggat ctctcgcgag cagcagggag agcattgggt
      181 tggctccgag tacgactccc tcgggcgccg caagaccgtg cggtcttcca agggactgat
      241 gcagcggatt cgccgcaact ccatgggcga tgtcgttgcg atcgaagcgg gtgtacacac
      301 gctgacgacc gaaggaacag acccttctac gctgggtcga atcttcggcg aacgcgaccc
      361 ctaccgcgtc gacttcgagc gcgaccgact cggactggag ctacaacgca acctacctgg
      421 cggtgtacag gcccgctggc aacgcgaccg cctcggacgg ccggagttgc atgagatccg
      481 agtgcacggg gtggtccaga gcgcccgccg ctacgaatgg gacagcaacg accgactgca
      541 ggcgatcctc gatgctctca aaggcccggt gcaatacagc catgacgcgc tcggcaacct
      601 ggctgcggca acctacgccg atggacgcgt cgaatggcgc atgcccgacg ccgtgggcaa
      661 cctgttccgc acccgagaac gtgacgaccg caaatacggc ccagcggggc agctactgga
      721 agcccgcgat gcgcgcggag tcacgaccta tgctcatgat gccgaaggca acctgatcga
      781 gaaaacggag ccggacggcg cgcgctggga atatcgctgg aacctatccg gcatgctcat
      841 cgaggtacgg cggcctgacg gcgacgtcgt gcgctttgcg tatgatgcat tggggcgaag
      901 aatatggaag gcgtatcgcg gtaagagcac ccactggatc tgggatggca atgttccctt
      961 gcacgagtgg atcacgctga acgacgaagc gttggcgcgc gacggcgcgc cgatcgcgac
     1021 ctcggcggag ctggcgattg cgatcgccaa acgtgaagcg ctgctcgcgc gaagatcggc
     1081 gcaggggccg ccggaggtgc cgacgttgga aggcaccgcg gactcgccga tcacctggtt
     1141 atttgagccc gagagcttct cgccgctggc caagctcgtc gccgatgagc gctactccat
     1201 cgtcaccgac catctcggca ctccgcgcgc catgttcgat gagcgcggaa gacagagctg
     1261 ggcggcggag acggatacct atggacggct gagtgacgtc agggggctgc gcggggcgtg
     1321 cccgtttcgg tttccggggc agtatgagga tgcggagacg gggctctact ataatcggtg
     1381 gaggtattat gacccggagg gtggggggta tgttagtcgg gatccgattg ggttggcggg
     1441 ggggcttgct tcttaccgct acactgctga cccacttttc tggtttgatg tttttggcct
     1501 cagtacttca tgcgggcatc atgcaattga gcaaacgccc attagccgcc gcaatgctcg
     1561 cagattgctg cgtgaccggg gcctatctaa caagcgcgcg gccgagcttg tcgatagctt
     1621 tgaggggcaa atctatgcgg taaagggtaa ggctggcgaa gtcttcgcaa tcaccgaatc
     1681 aaatcccggc gccgcgtcgg ctgtgttcgt gaccagggat tttgttgctc ctagccctac
     1741 tgagcgagca agcaagcttg ccctaccacc ttcgaacgct gctgaggtcg agggcttggt
     1801 tgaactcaca agagaccagc ttcttctcga gggccgagtg gcaccacagc tccagtgggg
     1861 tgctgataaa accgggggcg cttggcagac ggtcacggat ggtggcagat acacgggagc
     1921 agtcaggaga atatagcgga tggcgagtcc acgcgcagcg ggtcaggatg tcctcgatct
     1981 gcttagggat catggcttct tcgacgatgc ggcccggcta gagcgagcac tttcgagctt
     2041 agactcgggc gctccacagg ttcgagcagc cgcccagcga gaaattttga acatgtgcca
     2101 tccgcgttgg ttaggagatc tttacatcga agggctgtcg ctgacggatt ggtcggggaa
     2161 gttggatgcg ttatcgaacg cggtccaagg ctcccatgcg caatcgaagg aggctcggag
     2221 aagatcttga gccgctgtct attgcctctg attcacggag tgcgttgagg ttcgatgcgc
     2281 cgcatacgaa gagcgcggca tgaggtaccc gtctcacgtt ggacggtgcc gccatcggcc
     2341 gccccgcgcc cccaatcccg ttcgtgtgtc tctccctcgc gcccgcgagg tgttcgcgtc
     2401 agctcgagtc gagcggcttc cagcgatggg gcatgagctc atcgactgag caagttggat
     2461 ctcgcccgag tcgccgaagt acgtcggtta agtacgcgat cggatcgatg cgcgccctct
     2521 tgcagcatgc ggtgagcgtg tagagggtcg cgatgctctg gccggcagat ttcgcgccga
     2581 cgctcatgta gttcttgcgt ccgagggcga cgattcgaag ctgtccttcg gacaggttat
     2641 tcgagacggg gatcttggcg ttcgacagga agcgcgtcaa ctcctttgga ttagctcaga
     2701 cttaatctga caaatagttg gcccacccgg tgttcgcgat cgagagaaag gaacatgaac
     2761 gtgagcaccg agcagaaagt gcggttgtca accacctacc agacgggaat tccggacgtc
     2821 agcaggtgtg agccaagcag ctgagcaggt gtgaggctat ggcggctcga gcgaggctac
     2881 gcgagcggca cgaggcgttc ggcgaggaca cggacgg
//
LOCUS       DASTKU010000474         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1875590, whole genome shotgun sequence.
ACCESSION   DASTKU010000474 DASTKU010000000
VERSION     DASTKU010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875590"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VFQ61_16425"
     CDS             <1..1137
                     /locus_tag="VFQ61_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956095.1"
                     /translation="HQNQLRGPHRRRKDDRPTGWARVNTTSHRTFGHWLRVYCVAAAT
                     LLAAPVAGAQQGDELSQAESAFRDIDFERCAAAAARALESGGRGLHETARLYSLLGIS
                     LAALGQHDEAMRAFVKMLAIEPEQRVESSLSPKLRAPYLDAKGFWGTTSERLRARVEF
                     GPHGVTVRITDPVGMASKLRIATRGSAESTWQTTNHAAAPVVELAHRAGDELEFTLSL
                     LDEHGNTIHELGSQGAPEHLRRPRSIARLAPSSALDRAGEEVRPSRAHPLPWVTAGLA
                     VLGAGATATGVYFHVQREARASEWNGPDCERPGLTRGAQCASIDEARRRDQTLAIGLY
                     TAGGTLLTGALVTYLLWPGSARTEAAFACSPSAAFLGCSYQRRF"
     gene            1476..2108
                     /locus_tag="VFQ61_16430"
     CDS             1476..2108
                     /locus_tag="VFQ61_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956096.1"
                     /translation="MRHTILGFSMTLGAALAGSISGCSSETGSSSTGADVASDEAALS
                     AAQGSLASAKEQAKACFDTFRSCREASGANVQACRDALKACLPAEAPKPPHCGAPSGQ
                     RGDKSGGDDAAAGSSGDEHEGGHGRACDRPPVDGGKVKACGARAGTAVDQGTSVDSAK
                     ATHADCMNKAFGDVFKKICERASSACADSKAPADVCARITEECASLPATP"
     gene            2557..>3119
                     /locus_tag="VFQ61_16435"
     CDS             2557..>3119
                     /locus_tag="VFQ61_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956097.1"
                     /translation="MTTGVTKGSFWVVVLRVMWVLAAFVACTSSSPQNDPFKPGTGGF
                     AQRCTKSEDCASGLCVRLDEKTGICSTACANASDCPSANNWACLPAGSSGLEVCACRL
                     LGTTEFCGDGADNDCDGRADDCRMCGGRPAPIDDPQNCGNCGTVCSGGHVCRNAECQC
                     PSADLVECGSDCIDTRSNPNHCGACNQR"
BASE COUNT          577 a          938 c         1030 g          574 t
ORIGIN      
        1 catcaaaacc agctacgcgg tccgcatcga aggcggaaag acgaccgtcc gacgggttgg
       61 gctcgagtga acacgacctc gcatcgaacg ttcgggcact ggctacgcgt ctattgcgtc
      121 gcagctgcca cgttgcttgc ggcgccagtt gccggagcgc aacaaggcga cgagctctcc
      181 caagccgaat cggcttttcg ggacatcgac ttcgagcgct gcgcggccgc tgcagcgcgc
      241 gccctcgaga gcggtggtcg aggcctccac gagacagcac ggctctattc tctgctaggc
      301 atttctttag ccgcgctcgg acagcacgac gaggcaatgc gcgccttcgt gaaaatgctc
      361 gcgatcgagc ccgagcagcg ggtcgaaagc agcctttctc ccaagcttcg cgcgccgtac
      421 ctggatgcca aaggcttttg gggcacgacg agtgaacgac tgcgcgcccg agtggagttc
      481 ggtcctcacg gggtcacggt gcgcatcact gacccggtgg ggatggcgag caagctacgt
      541 atcgcgactc gcggcagtgc cgaatcgacg tggcagacga cgaaccacgc tgccgcgccg
      601 gtcgtcgagc tagcccaccg cgccggtgac gagctcgagt tcaccttgtc tctcctggac
      661 gagcacggga acacgatcca cgaacttggc tcgcaaggcg cacctgagca tctgcggcga
      721 ccacggtcca ttgcgcggct tgctccgagc tccgctctcg accgcgcggg cgaggaggtt
      781 cggccatcac gcgcccatcc attgccttgg gtcactgcgg ggctcgcggt tctcggcgcc
      841 ggcgccacgg ctacgggagt ctatttccac gttcagcgcg aagcccgcgc gagtgagtgg
      901 aatggcccgg attgcgagcg gcccgggctc acccggggcg cccaatgcgc ttcgatcgat
      961 gaagcgcgac ggcgcgatca aacccttgca atcgggctct acacagctgg tggaaccttg
     1021 ctcacgggcg cgctcgtcac gtacttgttg tggcccggtt ccgcgcgcac ggaggcagcc
     1081 tttgcttgct ctcccagtgc agctttcttg ggatgtagct atcagcggcg cttttagcgt
     1141 ctttaactcg ttgcgccggt aggtgttagc tctttgcgcg tcttgaagag ttgtatcgga
     1201 gcttgccacg aaggctaaag gatattgcga tcgcgactcg actgccgacg cgaattgcac
     1261 ggggctgagt cagggcggcg cgtccgtaac tgccgcgcgg ttcacccagg cagcgtcgtt
     1321 cgaattccta cgaattcgcg cacccgaacc aaaaagtgcg aacagggtga tcggttttgg
     1381 tttccgcggc gcacgatagt cgcgaaacga gcgcccaagg gtccggcacg ttgccgggcc
     1441 agggcactcg tctacgcatt ggagcaagta tgtcgatgag acacaccata ctgggatttt
     1501 ccatgacgct cggggctgcg ttggcgggat cgatttccgg atgcagcagt gagacgggca
     1561 gtagcagcac cggcgcggac gtggcgagcg acgaggccgc gttgagcgca gcgcaaggca
     1621 gcttggcttc cgcaaaagag caagccaaag cctgcttcga tacctttcgc agctgtagag
     1681 aggccagcgg cgccaacgtt caggcctgtc gagacgcgtt gaaggcgtgt ttgccagcgg
     1741 aagcgccgaa gcctccgcat tgcggagcac cgagcggaca acgcggcgac aaatccgggg
     1801 gcgacgacgc agcggcgggc agctctggtg acgagcacga aggcgggcac ggccgcgcct
     1861 gtgacaggcc gcccgtcgac ggaggcaagg tcaaggcatg cggcgctcgg gcaggcactg
     1921 cggtcgatca gggcacgtcg gtcgattccg cgaaggcgac tcacgccgat tgcatgaaca
     1981 aagcgtttgg cgacgtgttc aagaagattt gcgaacgcgc ttccagcgcg tgcgccgact
     2041 cgaaagcacc ggctgacgtg tgcgcgcgaa tcaccgagga gtgcgcctca ctgccagcca
     2101 cgccgtagtg ctcaatttca gaatgaacga tagttgatcg taggagcctg gcctcgccgg
     2161 gctgcaaatg acgaaggcga agccgtacac tcaatcgtgg gacttcgcct tcggttttgg
     2221 cgggccaatc gcgccccacg agacgacgct ggcaatgagt gaacacgcca tcttccgctt
     2281 cgaggaggct ggggctggga caaccgcttc aattgcttcg gtaccgaatg cgtcgctcgg
     2341 tttcgcgcgc atgtgtgtcg gagaacaaat cgaaaggatg gggtagtcgc actcgagaag
     2401 ttgagctcgc ttgtcaagcc cgctcaacgc ttctgacgct tcggccgtca tccgtcatga
     2461 gcgtctcgta cgtaggtcac gagctcccgc tctaggtcgc aacgcgagcg ttagttctgg
     2521 atgaagttgg agggttcggg taggaaggcg ggttgaatga cgaccggtgt gaccaagggc
     2581 agcttctggg tcgtagtgtt gcgggtcatg tgggtactcg cggctttcgt cgcctgtacg
     2641 tcttcgagcc cacagaacga tcctttcaag cccggcacgg gaggcttcgc gcagcgctgc
     2701 accaagagtg aagattgcgc ttctggcctt tgtgtgcgct tggacgaaaa gactgggatt
     2761 tgcagcaccg cgtgcgcgaa cgcgagtgac tgtccctcgg ccaataactg ggcgtgcttg
     2821 ccagcagggt ccagcggact cgaggtgtgc gcctgccgtc tgctcggcac gaccgagttt
     2881 tgcggtgacg gtgccgacaa cgattgcgac ggtcgcgcgg atgactgccg gatgtgcggc
     2941 ggcaggccag cgccgatcga cgatccgcaa aattgcggca actgcggaac cgtctgctcc
     3001 ggcggtcacg tctgtcgtaa cgcagagtgc cagtgccctt ccgccgacct cgtcgagtgc
     3061 ggttcggatt gtatcgatac ccggagcaac ccgaaccact gcggtgcttg caatcagcg
//
LOCUS       DASTKU010000475         5063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1651958, whole genome shotgun sequence.
ACCESSION   DASTKU010000475 DASTKU010000000
VERSION     DASTKU010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651958"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFQ61_16440"
     CDS             <1..302
                     /locus_tag="VFQ61_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956098.1"
                     /translation="LAPGPRDMMKLVYRSVTIRGFVMGEFMHEAERARADLKRWMVEG
                     RLKYRVDTRTGFLTLPHAFMSLFRGENTGMLIVESTGSGESCRSASALLDQLARR"
     gene            310..864
                     /locus_tag="VFQ61_16445"
     CDS             310..864
                     /locus_tag="VFQ61_16445"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HET9956099.1"
                     /translation="MDDEQRTASSKFLSYVLRHEPGAIGVALDAKGWVDIDVLLAGCA
                     KHGRAISRAELDEVVINSPKQRFAISEDGRRIRANQGHSVSVDLGYEPVAPPDVLFHG
                     TVARRLPSIREKGLRSMQRHHVHLSADEATARIVGSRRGKPVILRIDARAMLGAGHVF
                     FLTPNGVWLTESVPSPFISGWDDE"
     gene            complement(839..1249)
                     /locus_tag="VFQ61_16450"
     CDS             complement(839..1249)
                     /locus_tag="VFQ61_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9956100.1"
                     /translation="MKLLGNGKLTAGFDADCGATREVLNRVGDKWSVLVVVLLGERAM
                     RFNELKRAIDGISQRVLTSTLRGLERDGLVRRTVYPTNPPQVDYALTKLGQTLLEPVS
                     VLAVWAQAHRAEVMQARDVFDRASEGLIRRPTRK"
     gene            1327..1791
                     /locus_tag="VFQ61_16455"
     CDS             1327..1791
                     /locus_tag="VFQ61_16455"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9956101.1"
                     /translation="MIDTTSQPLARAVAKDSVPEGSRSSRPLDGVLWIIQGLLGAMFV
                     GTSVWKLTTPLPELAAKMPWMGEVSPSFVYATAVLDLLGGLGVLLPSLTRVRPQLTVL
                     AALGCVALMIGAILFHISRGELANTPFNFLVAALSAFVAWGRHGRGAIAARA"
     gene            complement(1894..4422)
                     /locus_tag="VFQ61_16460"
     CDS             complement(1894..4422)
                     /locus_tag="VFQ61_16460"
                     /inference="COORDINATES: protein motif:HMM:NF019271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EGF domain-containing protein"
                     /protein_id="HET9956102.1"
                     /translation="MKRWVSTGLMGFALSTMALALPGCSVDGKRKYGGPAEADAGETS
                     ASGGLTGASAGGRGGSAGTSPSSGRGGGGGHTEPATRGDAPEETGEGGNAEAVFGCGG
                     IVCAPAATCLRAGDEARCECPQGYTDPRADGTLCQDIDECANLNGGCNALVTCTNSPG
                     SFACGACPSGYTGTPETGCVDIDECATDNGGCDALATCTNTPGARVCGNCPFGYSGSG
                     ESTCTDIDECATNNGGCDKAVTCVNKPGGFTCGDCPPGHSGGGASGCVDIDECATNNG
                     GCDSHVTCSNSVGSFSCGACPSGYTGTGSSGCVDVNECATNNGGCDGNATCTNTSGSR
                     TCACRTGYTGDGTVCKDVNECATNNGGCDKNAECANTTGSRTCACKTGYSGDGTTCND
                     VNECATNNGGCSTNAVCTNTTGGRSCACKSGFAGDGLTCTAAGAGCRWNCSGSNCVQV
                     ALDQDNDGHGTTACAAAPGDDCNDSDEAVYPGATELCDGIDNDCDRKVDLSDGLPLVG
                     AIKSAPGRNHTAVASAGDGSFGVVSTSSDMAGLLYGTISASGVGSFTTSPMFPPSTDN
                     VYLDPHLAWGSAPGQWGVAYGRNGRGGLAGYAGVMAFTSCCWQDINPPGAGQNYPGRV
                     DVTARGQGDLLFAGAGFGNLYLATQSSSGTTPLTSSLAVSSWDTYTPRVASSGTGSGV
                     IWQTQSPRALNWSAISASLQFGATEQLSTGAFSTDIAAITAGYGIAWIEGTGFRFMIK
                     KPNGATQCTSSVIPFGAVAASQGIALGDSGKGTVVVATSPDTNAIHLYRFDNACKVID
                     DTDVSTGASAPTEPRVVHGGTNVVVYWNEGTTGRYRFLSDSLCH"
     gene            4717..>5063
                     /locus_tag="VFQ61_16465"
     CDS             4717..>5063
                     /locus_tag="VFQ61_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9956103.1"
                     /translation="MESSDLGLLVSLDALLQEGSVTGAARRIGLSTPAMSHALARIRA
                     RLGDEILVRSGRGMLLTPRALALKPQVHQVVAEARGVLEPEKAFIPSELTRTFVVHAT
                     DYVLTVIGTAVDR"
BASE COUNT          884 a         1669 c         1580 g          930 t
ORIGIN      
        1 cgctcgcgcc tgggccgcgc gatatgatga agctcgtgta tcgcagcgta acaatccgtg
       61 gcttcgtgat gggcgagttc atgcacgagg cggagagggc gcgcgccgac ctgaagcgct
      121 ggatggtgga ggggcggctc aagtaccgcg tcgacactcg cacgggtttt ctcactcttc
      181 cgcacgcgtt catgagcctg ttcaggggag aaaatacggg catgctcatc gtcgaatcaa
      241 ccgggtcggg cgagagctgc cgctccgcat ctgcgctgct cgaccaactc gcaaggagat
      301 aatcggggta tggacgacga acagcgcacc gcctcgagca aattcttgag ctacgtgctt
      361 cgccacgaac ccggcgcgat cggcgtcgct ttggatgcca agggctgggt ggatatcgac
      421 gtcctgctcg cgggctgcgc gaaacacggc cgggccatat ctcgggcgga gctcgacgag
      481 gtggtcatca acagccccaa gcagcgcttc gccatctcgg aggatgggag gcgcatacga
      541 gcaaaccagg gacactccgt gtcggtagat ctcgggtacg agccagtggc gcctccggat
      601 gttctctttc acggcaccgt tgcccgccgg ttgccgtcga tacgcgagaa ggggctacgg
      661 agcatgcagc gccaccacgt tcacctctcg gccgacgaag ccaccgcccg aatcgtgggt
      721 agccggcgag ggaaaccagt gattctgcgg atcgacgcgc gagcgatgct cggcgccgga
      781 cacgtgttct ttctgactcc gaacggagtc tggctgaccg agtccgtacc gagcccattc
      841 atttccgggt gggacgacga atgagccctt cagacgcgcg gtcgaacaca tcccgcgctt
      901 gcattacttc ggcgcggtgg gcttgcgccc acaccgcgag cacgctgacg ggctcgagca
      961 gcgtttgacc gagcttagtc aacgcatagt ctacctgcgg tggattcgtg gggtagacgg
     1021 tgcgcctcac gagaccatcg cgctcgaggc cacgaagcgt cgacgtgagc acccgttgcg
     1081 agataccgtc gatggcgcgc ttcagctcat tgaagcgcat cgcacgttcc cccagaagta
     1141 cgaccaccaa aacactccac ttgtcaccga cgcgattcaa cacttcgcgg gtggctccgc
     1201 agtcggcgtc gaaccccgcg gtgagcttgc cgttgccaag taacttcaaa gtgcctcctt
     1261 cacacggtca tggtggtgcg tatcttggct ctggtcgaaa ttggtaacct tcattcgaga
     1321 atcagcatga tcgatacgac ctcgcaaccg ctcgcacgcg cagtggccaa agattcggtg
     1381 cccgagggtt cgcgctcctc acgcccgctc gacggggtcc tatggatcat ccaagggctc
     1441 ctgggcgcca tgttcgttgg aaccagcgtc tggaagctca ctacacccct gccggagctc
     1501 gcggcgaaga tgccgtggat gggggaggtg tcgccgagct tcgtttacgc caccgccgtg
     1561 ctcgacctcc ttgggggact cggcgtgctg ctgccgtctc tgactcgcgt ccgaccacag
     1621 ctcaccgtac tcgccgcgct cggctgcgtc gcactcatga ttggcgcaat cctcttccac
     1681 atctcgcggg gcgagctggc caatacgccc ttcaacttcc tcgtcgcggc gctgtcggcg
     1741 ttcgtggctt ggggacgcca tggccggggc gcgatcgctg cgcgtgccta atcacttcgg
     1801 ttcgacgctg agtggcggcg cccaagagca ggggaggacc ctcggctcca gggcgctcac
     1861 tcgttttggc ggcgcaccaa acgggggggc ccctcagtgg cagagtgaat ccgagaggaa
     1921 gcggtaacgg ccagtcgtgc cttcgttcca gtagacgacc acgttggtgc cgccgtgcac
     1981 gactcgcggc tcggtcgggg cgctcgcacc ggtactcacg tcggtgtcat caatcacttt
     2041 gcaggcgtta tcgaagcgat acaggtgaat ggcgttggtg tccggtgagg ttgcgacgac
     2101 cacggtgccc ttgccggagt cccccagcgc aattccctgg ctggccgcga cggcgccgaa
     2161 cggaatcacg ctcgaggtgc attgagtggc accgttcggc ttcttgatca tgaagcggaa
     2221 tcccgtgcct tcgatccatg cgatcccgta tccggcggtg atggcggcga tatcggtgga
     2281 gaacgcgccc gtggacagct gttcggtcgc accaaactgg agcgaagcag agatcgccga
     2341 ccagttgagg gcacgcggtg actgagtttg ccaaatcaca cccgaacccg tgccgctgga
     2401 cgcgacccgc ggtgtgtacg tatcccagga tgaaaccgcg aggctgctcg tgagcggggt
     2461 agttcccgac gagctttggg tcgcaagata cagattgccg aagccagcgc cggcgaacag
     2521 cagatcaccc tgtcctcgag cggtgacatc gactcgaccg gggtaattct ggcctgctcc
     2581 cggcggattg atgtcctgcc agcagcaact cgtaaacgcc atcacgcctg cgtacccggc
     2641 gagcccgccg cggccatttc ggccgtaggc tacgccccat tgacctggcg cactgcccca
     2701 agcaaggtgc gggtcgagat acacgttgtc cgttgacgga gggaacatcg gactggtcgt
     2761 aaagctcccg acgccgctcg cgctgatggt gccatagaga agcccagcca tatcggagga
     2821 ggtactcacg actccgaagc taccgtcgcc ggccgacgcc accgccgtgt ggttgcggcc
     2881 cggcgcgctc ttgatagcgc ccaccaacgg gagcccatcc gacagatcca cctttcggtc
     2941 gcagtcgttg tcgataccgt cgcaaagctc tgtcgcgccg gggtaaacgg cctcatccga
     3001 gtcgttgcaa tcgtcaccgg gagccgctgc gcaggccgtc gtcccgtgcc cgtcgttgtc
     3061 ctggtccagc gcgacctgga cgcagttgga accgctacag ttccagcggc agccagcccc
     3121 cgcggccgta caagtcaacc cgtcacccgc gaaccccgat ttgcaggcgc agctgcggcc
     3181 gccggtggtg ttggtgcaaa cggcattggt gctgcagccg ccgttgttgg tggcgcactc
     3241 gttcacgtcg ttgcaggtgg tgccatctcc ggagtagccg gtcttgcaag cgcaggtgcg
     3301 ggagccagtg gtattggcgc actcggcgtt tttgtcgcag ccgccgttgt tggtggcgca
     3361 ctcattcacg tccttgcaga cggttccgtc gcccgtatag cctgttctgc aagcacaggt
     3421 gcggctgccc gaggtgttgg tgcaagtcgc gttcccatcg cagccaccgt tgttggtggc
     3481 acactcgttg acgtcgacgc agccgctcga tcccgtgccc gtgtacccgc tcggacaagc
     3541 gccgcacgaa aaagaaccga ccgaattgct gcaggtgacg tgtgaatcgc aaccaccgtt
     3601 gttggtggca cactcgtcga tatcgacaca gccggatgca ccaccgccag agtggcccgg
     3661 cgggcagtca ccgcaagtga agccgccggg cttgttgacg caagtcaccg ccttgtcgca
     3721 accaccgttg ttggtcgcgc actcgtcgat atccgtgcac gtcgactccc cgctgccgct
     3781 ataaccgaag ggacagttcc cgcagacgcg tgcgccgggc gtgttggtgc aggttgcaag
     3841 cgcgtcgcag ccgccattgt cagtcgcgca ctcgtcgatg tcgacacaac ccgtctccgg
     3901 cgtcccggtg taacccgacg ggcaggcccc gcaggcgaac gagcctggcg agttggtgca
     3961 cgttacgagt gcgttgcaac cgccgttcag gttggcgcac tcgtcgatgt cttggcatag
     4021 cgtgccgtcc gcgcgcggat ccgtataacc ctgggggcac tcgcagcgcg cctcgtcgcc
     4081 agcgcgcagg cacgtggcag cgggagcgca cacaattcct ccgcagccga acaccgcctc
     4141 cgcattcccg ccctcaccgg tctcctccgg agcatctccc cgagtagctg gctcggtatg
     4201 accgccgccc ccgcctctcc cgctgctcgg cgaggtgcca gcggaacccc cgcggccccc
     4261 ggcggaggct cccgtcagcc cgccgctcgc cgaggtttca cccgcatccg cttctgccgg
     4321 ccctccgtac ttgcgcttgc cgtccaccga gcagcccggc agcgcgagag ccatcgtgga
     4381 cagcgcgaac cccatcaaac cagtagacac ccacctcttc atcgtttgct ccttacacac
     4441 gacaccgact gcgatcccaa acccagacct cgctctatcg cggaatcgtc ccgagccaaa
     4501 ggtgtttcga ccgcgccatg acttttagga cgtctccgaa tgcgacgagc gccgaatgtg
     4561 ataacgttat cgcaatccta tcacagcccg caacgacctc accccaaagt atcgtgccac
     4621 ttgggcaggc atgccgtgtg gtaacgttat cgaaacgcca gcctgggcgg aaaccggctt
     4681 gatggctgcg accccggctg gccatagtcg tagcccatgg aatcatcgga cttgggcttg
     4741 ctcgtctcgc tcgatgctct gctgcaagaa ggcagcgtca cgggtgcggc gcgccgcatc
     4801 ggcctttcga ctccggcgat gagtcacgca ctggcgagaa ttcgtgcgcg gctcggggac
     4861 gagatcctag tacgctccgg acgcggaatg ctcctgaccc cgcgtgcgct cgccctcaag
     4921 ccgcaagtgc accaggtggt tgcggaggcc cgaggtgtgc tcgaaccgga gaaggcgttc
     4981 atcccgagtg agctcacgcg aacgttcgtc gtgcacgcca cggactatgt gctgaccgtg
     5041 atcggtactg ccgtggatcg cat
//
LOCUS       DASTKU010000476         3811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_536928, whole genome shotgun sequence.
ACCESSION   DASTKU010000476 DASTKU010000000
VERSION     DASTKU010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536928"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFQ61_16470"
     CDS             <1..669
                     /locus_tag="VFQ61_16470"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9956104.1"
                     /translation="RRFIEAGPAAWLPASGAGAHPLLPNSGFSSEWNKFIWASQDELV
                     ASPAYAHLSDDPNVPMVQLNWYQAFAFCIWDGGRLPTEAEWEYAASGGDANRPYPWGF
                     DPPNHDYVDSGLCAQRDCTQGPLWRPVGTHWLGGGRFGHQDLEGSSGEWVLDYGDSSH
                     SVTVQRSTPCDNCANLTPYSDRIVRGGYPNAIDNRLCSTYRIGLPPASSESYYSAFRC
                     ARDL"
     gene            complement(687..1376)
                     /locus_tag="VFQ61_16475"
     CDS             complement(687..1376)
                     /locus_tag="VFQ61_16475"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET9956105.1"
                     /translation="MKALIQLATFTLASSLSSLALAEEPTPSRIVGTAPSQALEISVS
                     GGFAQGFGDVSKTTRISDFARDGASLQLGLGYRLSPRFMLGVYAEGAGYEHGALNSKE
                     TDSVGAAAGIQVQYHFAPFQRVDPWLSAGVGWRGYWETEDDSVKRSLQGVDSLRLQAG
                     VDCRLTPQIALSPVLGVSIAKFLAEKQPGQSDFHEIDSPRSNVFLFAGAMGRFDLGGL
                     SYRNPTQVASR"
     gene            1693..3357
                     /locus_tag="VFQ61_16480"
     CDS             1693..3357
                     /locus_tag="VFQ61_16480"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956106.1"
                     /translation="MTATTIGVVIPLPEEMRAFSESFSEDHWTMTSEMRGARVFYTYR
                     PPSAACTQRELRIVVTVQEAMGNGPAQEAASAMISHYTPDVIVGLGICGAIHKDLRIG
                     DVVVVNAVFDVIDAAKVEDPAPSAGGSARPDSLNFELKLMSRPQVPTPLIVNRASQFE
                     WWAKDEFEVWQRQCFERLPKFGLDPTTTSPRNDVHGERREEVWTHGKPQILCGASASG
                     FVMEALTFLKVHAFNRELRIVETECAGVARACRGEHVLTPYVFLRVVTDYADKTSRGN
                     EGARKTFPDDASAEQKREHEQALLAQQKDQALKREENDKRRQWATRNVMGLFRELAKK
                     HEFIESVHGAIERQWDASVLLGRFRESREIEFPRNRALALAQVFTALVNDSSDSAPPD
                     RKRLCISAESLALTAVNWEFAKANRNNVLEVWVLRMSPALVEGLAGWKVMSPDVARRF
                     EINLERLRDSFPYARFRVGEAPNVTPFHGTFHGDTFWIGHWEVDENIHKLTSSTGFKL
                     INGRNAPDLAEHYRNLFVQAFLDAKNVRELASREEERAAERRDNVS"
     gene            complement(3454..>3811)
                     /locus_tag="VFQ61_16485"
     CDS             complement(3454..>3811)
                     /locus_tag="VFQ61_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956107.1"
                     /translation="ALPKFDLERFDRIEDYALALLYAHGAYVIAWAPPRSLEELGAEA
                     GTLKEVLLGDAPVQAARKIFESRHLTDIRSGTGYRDLGISSRSCGCTGRAGPSWKEER
                     RSRPQSWRRRSCWQTD"
BASE COUNT          717 a         1227 c         1170 g          697 t
ORIGIN      
        1 cgtcgcttca tcgaagcagg cccggcggcg tggctgcccg cttcgggcgc cggcgcgcac
       61 cccttgctcc cgaacagcgg cttcagctcc gaatggaaca agttcatctg ggcttctcaa
      121 gacgaactcg tcgcttcgcc cgcgtatgct cacctgagcg acgatcccaa cgtgccgatg
      181 gtccagctca attggtacca ggcttttgcc ttctgcattt gggacggcgg gcgcttaccc
      241 accgaggccg aatgggagta cgccgcgtcg ggcggcgatg ccaaccgacc ttatccgtgg
      301 ggcttcgacc cacccaacca cgattacgtc gattctggtc tctgcgcgca gcgcgactgc
      361 acgcaaggcc ccctgtggag acccgttggc acccactggc tcggcggtgg ccgcttcggt
      421 caccaagacc tcgaagggag ctcgggcgag tgggtactcg actatggaga ctccagccac
      481 tccgtcaccg tccagcgctc caccccctgt gacaattgcg ccaatctgac tccttacagc
      541 gaccgcatcg tgcgcggcgg ctacccgaac gcaatcgaca accgcttgtg ctccacctac
      601 cgcatcggcc tccccccggc atccagcgag tcctactaca gcgcatttcg ctgcgcgcgc
      661 gacctctaga acgtcgccaa gcggtctcat cggctcgcga cttgagtcgg gttgcggtag
      721 ctcaggccgc ccagatcgaa acgccccatg gcgccggcga acaagaacac gttggagcgc
      781 ggcgaatcga tttcgtgaaa gtcggattgg cccggttgtt tctcggcgag gaatttggcg
      841 attgagacgc ccagcacggg gctcagcgcg atttggggcg tcaggcgaca gtcgactcct
      901 gcttgcagac gaagcgagtc cacgccttga agcgagcgct tcaccgaatc gtcttcggtt
      961 tcccagtagc cgcgccagcc gacgccagcg ctcaaccagg ggtctactct ttgaaacggt
     1021 gcgaaatggt attgaacttg gatgcctgcg gcggccccga ccgaatcggt ctccttcgag
     1081 ttcagcgcgc cgtgttcgta gccggcgccc tcggcgtaga caccgagcat gaagcgcggg
     1141 ctcaatcgat acccgaggcc cagctgcagt gaggcgccgt cgcgagcgaa gtcgctgatg
     1201 cgcgttgttt tcgacacgtc gccgaagcct tgcgcgaaac cgccggacac cgaaatctcc
     1261 aaggcctggc tcggcgcggt gccaacgatg cgcgaggggg tgggttcttc ggctagcgcc
     1321 agcgagctca gactggaggc gagcgtaaag gtagccaatt gaatcaaagc tttcatgggg
     1381 acttccctgt gtgccgcgtg ggtgtgcggc acctgcactc gcataagcac gagccgagcc
     1441 aaggccacgc caagacctcg gacaggcgag cggcggaaaa accgcgcgat cgagaatccc
     1501 tgtgcgtgca gtcgtccgtt gggcgcgcgt cgtcggcaac acctgtccgg ctagtgaacg
     1561 gacacgtgcc actccgccgg tttcagcttg cttgcctagg cggtcagcat cgagctcggt
     1621 ggcgcggcgc ttgtctcggg taatacgata tccgctgcga agcgaaagcg cgtgatgtat
     1681 agtcccgctg tcatgacggc aacgacgatt ggggtagtca ttccgctccc ggaagagatg
     1741 agggctttca gtgagtcgtt ttccgaagac cactggacca tgaccagcga gatgcgcggg
     1801 gcgcgcgtct tctacactta ccgccctccg agcgccgcct gtactcagcg cgagctccgc
     1861 atcgtcgtaa ccgttcaaga ggcgatggga aacggccccg cgcaggaagc cgcctccgcg
     1921 atgatctcgc actacacgcc ggatgtcatc gtcggcttgg gtatttgtgg cgcgattcac
     1981 aaggatctgc gcatcggaga tgtcgtcgtc gtaaacgcag tgttcgacgt gatcgacgcc
     2041 gcgaaggtcg aagatccggc gccgtctgcc ggcggcagcg ctcgacccga cagtctgaat
     2101 ttcgagctca agctgatgag ccgtcctcag gtgccgactc cgctcatcgt gaaccgagca
     2161 agccagttcg agtggtgggc gaaggacgag ttcgaggttt ggcaacgtca gtgttttgag
     2221 cggcttccga agttcggttt ggaccccacc accacctcgc cgcgcaacga cgtgcacggg
     2281 gagcgccggg aggaggtgtg gacgcacggc aagccgcaga ttttgtgcgg agcatcagct
     2341 tccgggttcg tcatggaagc gctgaccttt ttgaaggtac acgctttcaa tcgagagctt
     2401 cgcatcgtcg agacggagtg tgcgggcgtc gcgcgagcgt gccgcgggga gcatgtcctc
     2461 accccgtacg tgtttctccg cgtcgtcacc gactacgccg ataagacctc tcgtgggaac
     2521 gagggagccc ggaaaacgtt ccctgatgat gcttcggcgg agcaaaagag agagcacgag
     2581 caagcgcttc tagctcagca aaaggaccag gcgctcaaac gcgaagagaa cgacaagcgc
     2641 cgacagtggg cgactcgcaa cgtcatgggg ctatttcgtg agctcgccaa gaaacacgaa
     2701 ttcatcgagt ccgttcacgg tgcgatcgaa cgtcagtggg atgcgtccgt cctgctgggc
     2761 cggtttcgtg agtcgcgcga aatcgagttc cctcgaaatc gagcgctggc actcgcgcag
     2821 gtcttcaccg cgctcgtgaa cgacagttcg gacagcgctc cgcccgaccg caagcgtctc
     2881 tgcatctccg ccgagtcgct cgcgctcacg gccgtcaatt gggaattcgc caaggcaaac
     2941 cggaacaacg ttctcgaagt atgggtcctg cgcatgagcc cggccctcgt ggagggcctc
     3001 gccggctgga aggtgatgtc ccccgacgtc gctcgacggt tcgagatcaa tttggagcgg
     3061 ttgcgcgact cgtttccgta cgcacggttc cgcgtcggcg aagcgccgaa cgtcactccg
     3121 ttccacggaa catttcacgg cgacacgttc tggatcgggc attgggaagt cgatgaaaac
     3181 atccacaagc tcacgtcttc gaccggcttc aagttgatca acggccgaaa tgctcccgat
     3241 ctcgcagagc actaccgcaa tctgttcgtg caagctttcc tcgacgccaa gaacgtgaga
     3301 gagcttgctt cgcgcgaaga ggaacgagcc gccgagcgaa gggacaacgt cagctagccg
     3361 cgcaggtagt cgccgacgac gtcccgttcc cgttcacgcg cggcgactac gctcgagtcg
     3421 tcggcccttg ctcgcggatc gccacgttca cgatcagtcg gtctgccagc aactccgcct
     3481 tctccagctc tgcggtcgag accgccgttc ttccttccag ctcggaccag cgcgcccggt
     3541 acatccgcac gagcgcgatg agatccccag atcgcgatac ccggttccgc tccggatatc
     3601 cgtgaggtgc ctactctcga aaatcttgcg ggcggcttgc accggcgcgt cgccgagcag
     3661 cacctccttc agcgtgcccg cctccgcgcc cagctcctcg agcgaccgag gcggcgccca
     3721 tgcgatcacg taagcgccgt gcgcgtacag caacgccagc gcataatcct cgatgcgatc
     3781 gaagcgctcg agatcgaact tcggcagcgc c
//
LOCUS       DASTKU010000477         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1876261, whole genome shotgun sequence.
ACCESSION   DASTKU010000477 DASTKU010000000
VERSION     DASTKU010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876261"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VFQ61_16490"
     CDS             complement(<1..1466)
                     /locus_tag="VFQ61_16490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9956108.1"
                     /translation="MRISLFCHSLLSDWNHGNAHFLRGVVTELVERGHRVKVYEAANA
                     WSPSQLAEQYGSAALLDAQRQYPALDIQRYAESDLDLETAIGDSELAIVHEWTPHDLV
                     SRIGAVGKRLGCRLLFHDTHHRAVTAPEEMRRYDLSAFDGVLAFGEVLRQIYLSRNWA
                     ERVWTWHEAADVRVFKPLPEISPERDLVWVGNWGDDERVRELQEFLIDPIARLNSSAR
                     VHGVRYPESALASLARANIEYAGWLPNYRVPDAFARARVTVHVPRNPYVIALPGIPTI
                     RPFEALACGIPLICSPWQDAEGLFRAGKDYLLARDGREMTEMLRLLLNEPEAARELAL
                     HGRNTVLARHTCAHRVDELLSICRELGLNTQVASGALEPESTRAPVEVARANLRVAPD
                     LGIQVRPAECAVGLRLVLPSDPRRQARISRVLAEFPAIRVEFSPFDAPVDPALTLEVF
                     EWARRDCDLVECDRIAAHAADSSAWLSLRGADLAEDVL"
     gene            complement(1726..>2466)
                     /locus_tag="VFQ61_16495"
     CDS             complement(1726..>2466)
                     /locus_tag="VFQ61_16495"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9956109.1"
                     /translation="FLLILHGLGGSGEQMAQVLNLKTFAQEKQFAYATPDGTPDSMGR
                     RFWNASRVCCNFDAKEVDDVARLTHLLEKAARTPQVDRNRMFVVGYSNGGFMAHRLAC
                     EHSGTVRGIVSMAAAGPVPPETCNPEKSVAVLEIHGDRDAIVPFEGGHLFSRPEYPVS
                     PRVREGLSPWAKRNGCRGELHTRNTVDLIEAMPGPDTEILGFDQCTGSAIQLWKVIGG
                     AHVLQLGPVARNAIWRFILETSEIPQSG"
BASE COUNT          469 a          817 c          733 g          447 t
ORIGIN      
        1 aacaatacgt cttccgcgag gtctgccccg cgcaaggaca gccacgccga gctgtccgcg
       61 gcgtgggccg cgattcggtc gcactcgacc aagtcacaat cacgtcgcgc ccactcgaag
      121 acctccaggg tcaaagccgg atccaccggc gcgtcgaagg gactgaactc cacccgaatt
      181 gccggaaatt cggcgagcac gcgggagatc cgggcctgtc ggcgcgggtc gctcggtagc
      241 accagccgta accccactgc gcactccgcc gggcgcactt gaatgccgag gtcgggcgcg
      301 acgcgcaggt tcgcgcgcgc cacctcgacg ggagctcggg tcgactccgg ctcgagcgcg
      361 cccgacgcca cttgtgtatt cagcccgagc tcgcggcaga tcgagagcag ctcatctacc
      421 cggtgcgcgc aggtgtgtcg tgccaacacc gtgttgcgac catgcagagc gagttcacga
      481 gcggcctcgg gctcgttcag caacaggcgc aacatctcgg tcatctcacg cccgtcgcgc
      541 gccagcaggt aatccttgcc ggcgcggaac agcccctcgg catcttgcca cggcgagcaa
      601 atcagcggga tgccacaggc cagggcttcg aagggccgaa tcgtggggat gcccggtaag
      661 gcgatcacat acggattcct cggcacatga acggtgactc gtgctctcgc gaacgcgtcc
      721 gggactcggt agttgggcaa ccaacccgca tactcgatgt tggcgcgagc caggctcgcg
      781 agggcactct cggggtaacg cacgccgtgg acccgcgcgc tcgagttcaa tcgcgcgatg
      841 ggatcgatca agaactcttg gagctctcgc acgcgctcgt catcacccca gttaccgacc
      901 caaaccagat cccgctcggg tgagatctcc ggcaatggtt tgaacacgcg cacatcggcc
      961 gcctcgtgcc aggtccacac gcgttcggcc cagttgcgag acaggtagat ttgccgaagc
     1021 acctcgccga acgcgaggac accgtcgaac gcactcaagt cgtagcggcg catctcctcg
     1081 ggtgcggtca ccgctcggtg atgcgtatcg tggaacagca agcggcagcc cagccgcttt
     1141 ccgacggcgc cgatgcggct caccaaatcg tgaggcgtcc actcgtgtac gatcgccaac
     1201 tcggaatcgc cgatcgcggt ctcgagatcg aggtcactct ccgcatagcg ctggatatcg
     1261 agcgcaggat attggcgttg cgcgtccagc agggccgcgc ttccgtattg ttccgccagc
     1321 tgcgaggggc tccaggcatt cgcagcctcg taaaccttca cccgatggcc tcgctctacg
     1381 agctcggtca ctactcctcg caagaagtga gcgttaccgt gattccagtc ggacagcagg
     1441 gagtggcaaa atagtgagat tctcatggcc cagcccggag gaaagctcgc cgtctagccc
     1501 gcgtgcctcg agcatgcggc tcatcgcgag tgtcggagtc gagtgccaat gcgactcaca
     1561 tgccaaactt gctgactcgc tggccaggta caaacctcgc gcacaacaca cgtctgcagc
     1621 ggtttggtcg cccggatggg gcagcaatgg tgtcgctcac cactgaccat tgacggtttt
     1681 ggtccggtca gcacgacact tcagctcgtt ttaacctcgt ccgggttatc ccgactgcgg
     1741 gatttcacta gtttccagga tgaagcgcca tatcgcgttc cttgcgacag ggcccagttg
     1801 caaaacgtgt gctcctccga tcaccttcca cagctgaatg gccgatccgg tgcactggtc
     1861 gaagcccaga atctccgtgt ctggcccagg catggcctcg atcaaatcca cggtgttccg
     1921 cgtgtgcaat tccccgcggc agccgttgcg cttcgcccaa ggggaaagcc cctctcgaac
     1981 gcgcggtgaa accggatatt cggggcggct gaataggtgg cctccctcga acggaacgat
     2041 cgcgtctcga tcaccatgta tttctaacac cgccaccgac ttctccggat tgcatgtctc
     2101 cggtgggacg ggccctgcag ccgccatgct gacgatcccg cggacggttc ccgagtgctc
     2161 acacgcgagg cgatgagcca tgaagccacc attcgagtac cccaccacga acatgcgatt
     2221 tcgatctacc tggggagtgc gggcagcctt ttcgagcagg tgagtcaagc gcgccacgtc
     2281 gtcgacttct ttagcgtcga agttgcagca gacacggctc gcgttccaaa accgccgccc
     2341 catgctgtcg ggagtgccgt ccggcgtcgc ataagcgaac tgcttctctt gggcgaacgt
     2401 tttcaagttc agcacctgcg ccatttgttc tccgctgccg cccaggccgt gcagaatcag
     2461 caagaa
//
LOCUS       DASTKU010000478         7133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1205900, whole genome shotgun sequence.
ACCESSION   DASTKU010000478 DASTKU010000000
VERSION     DASTKU010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7133
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205900"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFQ61_16500"
     CDS             complement(<1..247)
                     /locus_tag="VFQ61_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956110.1"
                     /translation="MRHNIHGFAAVGWVLVAAFGCSGSDGLQGTDGAQGPSGAHAVVR
                     TSDKVDLNLCRNGGQRIEYGKDDNANGMLENAEVDGHR"
     gene            complement(262..2349)
                     /locus_tag="VFQ61_16505"
     CDS             complement(262..2349)
                     /locus_tag="VFQ61_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956111.1"
                     /translation="MSRVCEARTRSGCGILVSNRSPHSRLTDHESRVTERGSGFGSGS
                     GFWNKVEAVLRTLALKLTGLVSILGCLSAGCGDGEDGAAGKVGPAGVDALVKTTDEPA
                     GTKCEHGGAKIQYGMDNNGNGALDASEIDGTSYACNGAPGQDGQDGPAGENGEPGEDG
                     TDGHDALIITTDEPAGENCVRGGTRFDQGIDEDGDGELAEDEILSSTYVCNGEDGEDG
                     ANGSDMLLDVSMEAAGQNCPAGGQKIQAGPDLDGNGSLSAGEVDNTSYVCNGGQGAAG
                     VGNLVRVTDEAAGSNCNASGVKLEIGRDVDANGELAATEVEVTRYLCNGGSAPAGLTS
                     LVNVTAEPAGTNCVVGGQKLETGLDVDRNNTLDAAEVLQTQYLCAGENGAPGATGANG
                     HASLVVITDESPGAQCSAGGKKIQAGLDANDDATLSPSEATRTDYVCHGLAGKDGADG
                     ADAHDGLLTVTDEAVGTRCPAGGKRIDVGTDLDDDKVLDAAEISATQYVCNGTNGVSG
                     MNGTNGSNGLVAVLDEPAGANCPAGGERISYGLDDDADGILDLGEIDGTRYVCDGVAG
                     TNGTNGAAGQNGFNSLVAVVNEPAGSNCVAGGEKITYGLDDNRDNTLDAAEVDGTRYV
                     CDGVAGANGTNGTNGTNGLNSLVSVTTENPGANCLAGGQRIQSGLDDDANGTLAAGEV
                     EATAYVCNGNSAI"
     gene            2797..3249
                     /locus_tag="VFQ61_16510"
     CDS             2797..3249
                     /locus_tag="VFQ61_16510"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9956112.1"
                     /translation="MRKPPVGWPRISSALFYENPGAAIDWLCDVFGFECRLKVEGEAG
                     RIEHSELTFGEGLIMVGASGGKSERPNPLPCQSPRSVAGVNTQSLCVFVDDVDAHCAR
                     ARARGARILDEPTTTDYGPDYWSDRSYRVADLEGHQWWFMQRMRDPQA"
     gene            3271..3621
                     /locus_tag="VFQ61_16515"
     CDS             3271..3621
                     /locus_tag="VFQ61_16515"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9956113.1"
                     /translation="MTRNLDSTFAALADPARRNIIELLQQRPRCPSEVADTLQMSRPA
                     MSRHLRILRSAGLIDQESSEQDARVRMIRLRRAPFSELRSWVDHVEAFWEDQLSAFKA
                     HAERTVERAPKRSR"
     gene            3618..4157
                     /locus_tag="VFQ61_16520"
     CDS             3618..4157
                     /locus_tag="VFQ61_16520"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9956114.1"
                     /translation="MSSDSPKRASDPSPTRPFDMARVSVLVHVDPDTAFRIFSEDIDQ
                     WWRRGYKYRASGADRGILYLEPRVGGRLYESFERAGVKRVVETGLVLEWSPPERLAFE
                     WRSVTFAPHERTHVEVRFEPQGDATLVTVTHRGFAQLPPEHPVRHGAQGAAFIGRMAL
                     WWSELVTSYRERSEASASS"
     gene            complement(4207..>7133)
                     /locus_tag="VFQ61_16525"
     CDS             complement(4207..>7133)
                     /locus_tag="VFQ61_16525"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9956115.1"
                     /translation="APVGTGNSAGSARQPEAQDSDVHVSTRSPGSASPAATQSIIAPD
                     PPFVGQVDAPRAQSVPVETDGAMRIGQYEVIRELGRGGMGVVYLARDERLGRKVALKL
                     LQTHHPEFTRRFITEARATARCSHENIVVIHEVGEHRGNPFMVLEYLEGQTLSALLKK
                     SGPLAPSRAAELMVSVVRALVCAHEQGIVHRDLKPDNIFITSSGAVKVLDFGIAKVLG
                     GERVFSRETGSLDRPAVNVDPARSGIAGTLAYMSPEQWGIGGSIDHRTDIWASGIILY
                     EMLAGVHPLASLGPAPQHWVARLDLPMPSVREAAPNTPEELAQVVDGCLRKVKTERVA
                     DAVALLRALEPFLPGRASMPGAGTAGRQMESGPYTGLRAFQEEDAGRFFGRDRETLAM
                     ATRIREYPLMAAVGPSGIGKSSFVRAGVVPLLKHSGENWQVLVVRPGREPVLSLAALL
                     APLVSSSPTLVGDLGAQREIAERLRNEPGYLGGALRASARRDRQKILLFVDQFEELYT
                     LGAELADRLAFTACLSGAADDALSPVRVITSIRSDFLGRLAEDPHFMNELSKGLFFLG
                     PPSTEGLREALVRPCELAGYRFESEAMVLDMVRHLEAVPGALPLLQFTAAQLWETRDV
                     VRRQLTEQSYRQLGGITGALASHADRVVAKLPSEQQALCRSLLLTLVTPERTRAVRNL
                     DELEELAPNRGEVAALVDHLVQARLLVIQTASSGGASVEIVHESLIGTWPTLRRWLEE
                     SHEDSVFLDQLRNAARQWHAKRHDAGLLWGGEMVDELARFQRRYRGELPEVTRAFATA
                     VFAQKARTARRNRWVVVGAVAALLGMLAAAMVALVVIRREQQVAEQNANSAERRLRQV
                     EAKERERQAAERRRLAEENLRRKAEEEVIRANSKIQLSNEELLQKNAELVVTLKRAEE
                     LRHQAETAQKNAQENESVARRAEDRARRAAGELEDMLKRERERSERLSAQLGSPLVEV
                     LQ"
BASE COUNT         1213 a         2425 c         2111 g         1384 t
ORIGIN      
        1 cccggtgccc gtccacctcg gcgttttcca acatgccgtt ggcattgtca tccttgccgt
       61 actcgatgcg ctggccccca ttccggcata agttgaggtc gactttgtcg ctcgttctca
      121 cgacggcgtg cgcaccgctc ggcccttgcg ctccatcggt accttgcagc ccgtcggacc
      181 ccgagcagcc gaaagccgcg acgaggaccc agccgacggc cgcgaaacca tgaatattgt
      241 gtcgcatgct gtggctcctg ctcagatcgc ggagttgcca ttgcagacgt atgcggtcgc
      301 ctcgacttca cccgcggcca gcgtgccgtt cgcgtcgtcg tcgaggccgg actggatgcg
      361 ttgacctccc gcgaggcagt tcgctccggg attttcggtg gttacgctga cgaggctgtt
      421 cagcccgttg gtgccgttgg tgccgttggt gccgttggcg ccggcgaccc cgtcgcagac
      481 ataccgcgtc ccatcgactt cagctgcgtc cagggtgttg tcgcggttgt cgtccaggcc
      541 gtaagtgatc ttctctccac ctgcaacgca gttgctacca gccggttcgt tcaccaccgc
      601 cactaggctg ttgaaaccgt tttgacccgc agcaccgttg gtgccgttgg ttccggcgac
      661 cccgtcgcag acataccgcg tcccatcgat ctcgccgaga tcgaggatcc cgtcggcatc
      721 atcgtcgaga ccgtagctga tgcgctcccc gcccgccggg cagttcgccc ccgctggttc
      781 gtcgagcacg gccacgagac cgttcgaccc gttcgtaccg ttcatgccgc tgacgccgtt
      841 ggtgccgttg cagacatact gggtcgcgct gatttctgcg gcatccagca ccttgtcgtc
      901 gtccaaatcg gtgccgacgt cgatgcgctt gccgccggct ggacagcgtg tgccgaccgc
      961 ttcatccgtg accgtcagta gtccgtcgtg cgcgtcagcg ccgtccgctc catctttacc
     1021 agccaggcca tgacacacgt agtccgtgcg ggtcgcctcc gagggcgaga gcgttgcatc
     1081 gtcgtttgcg tccaggccgg cttggatctt tttgccgccc gcagaacact gcgcgcccgg
     1141 tgactcgtcc gttatcacaa cgagcgatgc gtgcccgttc gcaccggttg cgcctggcgc
     1201 gccattctca cccgcacaca ggtactgtgt ctgcagcacc tccgcggcgt cgagcgtgtt
     1261 gtttcggtcg acatccagcc cggtctccaa tttctggccg ccgaccacgc aattcgtccc
     1321 tgcaggctcc gcggtcacgt tcaccaagct ggtgagccca gcgggtgcgc tgccgccgtt
     1381 gcacaagtag cgggtcacct cgacctcggt tgcggcaagc tcaccgttgg cgtccacgtc
     1441 gcgcccgatt tcgagcttca cgccgctggc gttgcagttc gaacccgccg cctcgtctgt
     1501 cacgcggacc aggttaccga cgcccgccgc accttgcccg ccattgcaga cgtagctcgt
     1561 gttgtcgact tctcctgctg aaagtgagcc attgccatcg aggtccgggc cagcctggat
     1621 cttttgcccg ccggcggggc aattttggcc tgcggcttcc atgctcacat cgagcagcat
     1681 gtcactgcca ttcgcgccat cctcaccgtc ttcgccgttg cagacatagg tggacgatag
     1741 gatctcgtct tctgcgagct ctccgtcacc gtcctcgtcg atgccctgat cgaagcgagt
     1801 gccgccgcga acgcagttct cccctgccgg ttcgtcggtg gtgataatca gcgcatcatg
     1861 gccgtcggtt ccatcttcgc cgggctcacc gttctctcca gcgggcccgt cttggccgtc
     1921 ttgcccaggg gcgccgttgc atgcgtagct cgtgccgtcg atctccgacg cgtcgagcgc
     1981 tccattcccg ttgttgtcca taccgtactg gatcttggcc cccccgtgct cgcacttcgt
     2041 tcccgcgggt tcgtccgtgg ttttgaccag cgcatctact ccagccggcc cgaccttccc
     2101 agccgcaccg tcttctccat ccccgcagcc ggcggagagg caaccgagaa tcgagaccaa
     2161 acccgtaagc ttcaaagcca aagtccgcaa gacagcctcc accttgttcc aaaaacccga
     2221 gccgcttcca aaacccgagc cgcgttccgt cactcggctc tcgtgatctg tcagtcggct
     2281 atgcgggctc cggttcgaca ccaagatgcc gcaaccggag cgcgtgcgag cctcgcagac
     2341 gcgggacatt gtcccttccg catggcgcgc tgagccttcc tctcagggct tcgcatgctg
     2401 ctacgagtcg gatcttagaa ccaagagaaa tcctcagcgg tgatctcaag ttcgtatggc
     2461 gatgcaagca attgtgagac tgcatatcac cgtcgcggac ggtgttggtg tcacctcacg
     2521 ccgcttcgcc gcgtgaggca tgcgcacgag gcctcgtggc aaacgcgcgt gccacggccc
     2581 gcaatcgcga ggagcctgcg ccatacacgc aacagcgtat taggagtctc cgaacgcggt
     2641 gcaccggcgg aattcgacgg gcagccttcc gaaaaggtct tgctagatgg ttttagtttc
     2701 tgttaactat ttcgaacaat cgcaattatg aatatctgac tgatcgagcg cgacaactcg
     2761 ttcgactcta ctcattcgac tttggagtct tcaaccatgc gtaaaccacc cgttggctgg
     2821 cctcggatct cctcggccct gttctacgaa aacccgggcg ccgcaatcga ttggctctgt
     2881 gatgtctttg gcttcgaatg ccgcctcaag gtcgaaggcg aagccggccg gatcgagcat
     2941 tcagagctta cgtttggcga agggctcatc atggtgggag cctcgggcgg caagtccgag
     3001 cggcccaacc ccctgccgtg tcaaagcccc cgttcggtag ccggcgtgaa tacccagtcg
     3061 ctgtgcgtgt tcgtcgacga tgtggacgcg cactgcgcac gcgcacgcgc acgcggcgca
     3121 cgcatcctgg atgagcccac gaccaccgat tacgggccgg actactggag cgatcgtagc
     3181 tatcgcgtcg cggatctaga aggtcatcag tggtggttca tgcagcgcat gcgcgaccca
     3241 caggcttgaa gcagcgctag tccgcacagc atgacacgca acctcgactc cacgtttgcg
     3301 gcgttggccg atccagcacg tcgcaacatc atcgagctac tgcagcagcg gccgcgttgc
     3361 ccaagcgagg tcgccgatac cctgcagatg agccgacccg ccatgagccg ccatctgcgt
     3421 atactgcgaa gcgctgggct cattgatcag gagagttcgg agcaagacgc gcgagtgcgc
     3481 atgattcgct tgcgtcgcgc gccattctcc gaactccgct cttgggtcga ccacgtggag
     3541 gcattttggg aggatcagtt gtccgcattc aaagctcacg ccgagcgcac cgtagagcgc
     3601 gcgccaaaac gcagccgatg agttccgatt ccccgaagcg agcaagcgac ccgagcccga
     3661 cgcggccgtt cgacatggcg cgggtcagcg tgctcgtcca cgtcgatccc gatacggcct
     3721 ttcgcatctt cagcgaagac atcgatcaat ggtggcgccg cgggtacaag tatcgcgcgt
     3781 cgggcgccga ccgcggaata ctctacctcg agccacgcgt cggcggccgc ctctacgaaa
     3841 gtttcgagcg cgcgggagtg aagcgcgtgg tcgaaacggg tctagtgtta gagtggtcgc
     3901 cacctgagcg gctggcgttc gagtggcgtt cggtaacgtt cgctcctcac gagcgcaccc
     3961 acgtcgaggt ccggttcgag ccgcaaggcg atgccacgct cgtgaccgta actcaccgcg
     4021 gttttgccca gttgccgcca gagcacccag tgcggcacgg cgcacagggc gcggctttca
     4081 tcggtcgcat ggcgctgtgg tggtccgagc tggtcacatc ctaccgggaa cgatcggagg
     4141 cttcggcgtc ctcttagcgc cagtcggggg aagtgataac cgaagccacg agccgcgagt
     4201 tcgggctcat tgcagaactt ctacgagcgg gctgccgagc tgcgcggaaa gccgctcgct
     4261 acgctcgcgc tcccgcttga gcatgtcctc gagttctccc gcggcgcggc gggcgcgatc
     4321 ctcggcgcgg cgggcgacgc tctcattttc ttgagcgttc ttttgggcgg tttcggcttg
     4381 gtgacgtagc tcttcggcac gcttgagcgt cacgaccaac tctgcgtttt tctgcaacag
     4441 ctcttcgttg gaaagctgga tcttactgtt ggcgcggatc acctcttcct ccgccttgcg
     4501 ccgcagattt tcttcggcca agcgccgccg ttcagcggct tgccgctcgc gttccttggc
     4561 ttcgacttgc cgcagtcgac gctcggcgct gtttgcgttc tgctcggcca cctgttgctc
     4621 gcgccgaatt acgaccagag ccaccattgc cgcagccagc atgcccagca gcgctgccac
     4681 cgcaccgacc acgacccagc ggtttcgtct cgcggttcgt gccttttggg caaacaccgc
     4741 ggtggcgaag gctcgcgtca cctcgggcaa ctcaccccga taccgacgct ggaagcgggc
     4801 gagctcgtcc accatctcac cgccccagag taacccggca tcatgccgct tggcgtgcca
     4861 ctgccgcgca gcattgcgca attggtctag aaagacgctg tcctcgtggc tctcctcgag
     4921 ccaacgacgc agcgtgggcc aggttccgat caaagactca tggacgattt cgacgctcgc
     4981 gccgccgctc gacgcggttt gaatcaccag caggcgcgcc tgcacgaggt gatccaccaa
     5041 agccgcgacc tccccgcgat ttggggctag ctcttccagc tcatccaagt tgcgtacggc
     5101 ccgtgtgcgc tccggagtca cgagcgtcag cagcagagag cgacacaagg cttgttgttc
     5161 actcggcaac ttggcaacca cgcgatccgc gtggcttgcg agcgcgcctg tgattccccc
     5221 cagctgacga tagctttgct cggtcagctg gcgtcgcacc acgtcgcgcg tctcccacag
     5281 ctgtgccgcg gtgaactgca gcaacggcaa cgcgcccggg accgcttcca gatgccgcac
     5341 catgtccagc accatggcct cggactcgaa gcggtagccc gcgagctcac aggggcgaac
     5401 caaagcctcc cgcagcccct ccgtactggg cggccctagg aagaacagcc ctttactgag
     5461 ctcgttcatg aaatgagggt cttccgcgag gcgcccgagg aaatcggagc gaatggacgt
     5521 aatcacccgt acgggagaca aggcgtcgtc ggcggccccg gatagacagg cggtgaacgc
     5581 gagccgatcc gcgagctctg cgcccaaggt gtacaactcc tcgaactgat cgacgaatag
     5641 caggatcttc tgccgatcgc ggcgagcgct cgctctcaga gcgccaccca ggtaacctgg
     5701 ctcgttgcgc agccgttccg cgatctcacg ctgcgcgccc aagtccccga ccaacgtggg
     5761 cgaagagctg accagcggcg ccagcaaggc cgccaacgag agcacgggtt cccggccggg
     5821 ccgaaccacc aagacctgcc aattctcacc ggagtgcttt agaagcggca ccacgcccgc
     5881 ccgaacgaag gaggacttgc cgatccccga cggcccgacc gccgccatca acgggtattc
     5941 acgaatgcgc gtcgccattg cgagcgtttc gcgatctctt ccaaagaacc tacccgcgtc
     6001 ttcttcttgg aacgcacgca accccgtata gggcccgctt tccatctggc gcccagcggt
     6061 gcccgcaccg ggcatggatg cgcgccctgg caggaagggt tccagcgccc gcagcaaagc
     6121 caccgcgtct gcaacgcgct ctgtcttcac tttgcgcagg cagccatcta cgacctgagc
     6181 caactcctcc ggcgtattcg gggcggcctc gcgcacgctg ggcattggaa ggtccagacg
     6241 agccacccag tgctgcggcg cgggacccag cgaggccaag gggtgaactc cggccagcat
     6301 ctcgtacaag atgatgcccg aggcccaaat atcggtgcgg tgatcgatac tcccgccgat
     6361 cccccactgt tccggggaca tataagcaag tgtgccggcg atgccgctcc gcgcgggatc
     6421 gacattcacc gcgggtcgat cgagagaacc cgtctctcgc gagaacaccc gctcccctcc
     6481 caggaccttt gcgattccga agtcgaggac cttcaccgcc cccgaactgg tgatgaaaat
     6541 gttgtctggt ttgagatcgc ggtgaacgat gccctgctca tgcgcgcaca ctagagcgcg
     6601 caccacggag accatcagct ccgcggcccg cgatggcgcc agaggcccgg acttcttgag
     6661 gagcgccgag agagtttgcc cttcgaggta ctcaagcacc atgaatgggt tgccccggtg
     6721 ctctccaacc tcgtggatga ctacgatgtt ctcgtgactg catcgcgcgg tcgcccgggc
     6781 ctcggtaatg aagcgacgag tgaactccgg gtgatgcgtc tggagaagct tgagcgcgac
     6841 cttccgaccc aatctctcgt cgcgcgccaa gtagacgaca cccatgccac cgcgtccgag
     6901 ctcgcgaatg acttcgtact gcccgattcg catggcgccg tcagtctcca cgggcacaga
     6961 ctgcgctcga ggagcatcca cttgcccgac gaacggtggg tcgggggcga tgatgctctg
     7021 ggtcgcagct gggctcgccg aacccggact gcgagtggaa acgtgtacgt ccgaatcttg
     7081 ggcttccggt tgtcgagccg atcccgcgct attgcccgtt cctacggggg cca
//
LOCUS       DASTKU010000479        24162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_982450, whole genome shotgun sequence.
ACCESSION   DASTKU010000479 DASTKU010000000
VERSION     DASTKU010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 24162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24162
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982450"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFQ61_16530"
     CDS             complement(<1..232)
                     /locus_tag="VFQ61_16530"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET9956116.1"
                     /translation="MTRYALLLRGVNVGTKNSLPMAELRSMLTKLGCADVQTYVQSGN
                     AVFSTQLRQAALTHAVESALEGYMGRPIATTLR"
     gene            complement(374..1168)
                     /locus_tag="VFQ61_16535"
     CDS             complement(374..1168)
                     /locus_tag="VFQ61_16535"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET9956117.1"
                     /translation="MLGRRRLLAAGVASALTAACDNWARPPAASAPRPTHWVDLTHTL
                     SPEFPFIPVQNKTFPFRLQPIATNAHDGVYANRWELTEHVGTHLDAPCHFLDGASSIE
                     QIDLGKLVLPAAIVSIAERVARDEDAVFDVADLLRWERVHGEVPRGGALFLHTGWASR
                     VTESGRFVNLDAHGVMHFPGFSPQVADLLIHQRAISGIGIDTLSVDPGRDTQYPVHKR
                     LSGANKWALECLTNLEAVPATGATAIVAAVKVRGASGAPARVLAFW"
     gene            complement(1170..1637)
                     /locus_tag="VFQ61_16540"
     CDS             complement(1170..1637)
                     /locus_tag="VFQ61_16540"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HET9956118.1"
                     /translation="MTNSVPSLFEWMGGMPAIERLVERFYSRVGEDATLGPVFAQMSP
                     SHARHVAAFLAEVFGGPSTYSAELGGHPHMIRKHLGRSLSERQRKRWVDLLLECADDL
                     GVPSDPEFRSALVGYLEWGSRLAVINSQPGADVTTEAPMPRWGWGEVKGPYLG"
     gene            2694..9827
                     /locus_tag="VFQ61_16545"
     CDS             2694..9827
                     /locus_tag="VFQ61_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956119.1"
                     /translation="MADLSLIDLITPYVLRGGGFGAWHPALAVLAVYEHEVALSAEGV
                     VIRGVVRFTGDIQPYIDPDRMVIGVESSNQEGHPLNDPGRRSPWIDVRDAKIEFQLTV
                     PRVASAIVAQAVTNIGVDAAFAGAAAVLNQFDINPNDAPPSDFPNTEFTLDFLLTTIV
                     LRPPFLRGAKREADGQLVPDPSNEQVKLTLPKIKMRLMQGSAVGSPLSAVLLSAGASG
                     LDDPGDLSVAELITMDPPYAFIGPSNVVGFGFRSAVLDLSNGSTPPQVLEQFGYDESW
                     TGIYLPEIRLFVAPNGARDLAVEGGARNLLIGIGQSAGVTGDFDLQVLNQGAGPLKLG
                     ARFYDATGRSYGLTRTSESEGTVQLPARTRMIIDVDGGRTPIMTSATFDGGAAQNGRE
                     HDIDLSSATQRNIVLQATDTSAPPRAASMTVRVSRLSAPALPPAGSQPEAQNSAVDLQ
                     TTSVTQGGVAVSAPRLRLVSETPTTATIALDRPGNPTWTVGGVPAGTSSTVTVDVAPG
                     ATVNVRAELPGTASAGSFTAYYRFDHPEPQEGSDYATNPSNSRTEPAPDEGASSNWFG
                     GSDSITALRGALVGVPNHAAVRIEGFASFEGPDTAQTRTYNTALAQRRADGLRAMIER
                     VVSDGQTPALAGRTYDFTPAPAANMTAWTTQGTSLATRRQYWKAVASWSPAPAAGTIT
                     EGTVSRRSNSGGGTDPIPVPDPAPASPPPQPPSWFRQLGAKVRIVRNQFVACEVFGKF
                     DFQTASENRLREGAGPGTELPTWEGLGSQNPADGLVDLRVVVQIDDATDTVTVTGYFG
                     ADPADRDGLALLGTRPNQPPDPGPKNFGLDFFGTSCVFLPLIAEGAGAVANEGALAEL
                     AVTAGGLGLCAVIAGLGWMRTERIIWYGGELAVVANARGEWTTTLLFDIETAISADFL
                     GLVKIARNAPLVVRYKAIGILFGSPPGQPAFQFRPMFDSSKGYSIDLSRPGAIEVAEP
                     LGQILQILGARIARNNPMIIEVDLGFAIDLGVVSIERARVRVTLNESPSVELTAFGAG
                     IDIPGALRGRGYMELSETEIKGQIDLTIVPVSVRVAAGVGVAQIPAAQGGPATGVIVS
                     LYVEFPVAIPLGASGLGIYGFLGLFAMHYARNEDEIDAGNQAPALAWLKATGGNPTDL
                     QFWKPKINNWAFGVGAILGTMGSSVIFNLKGMLMLELPGPRLLLMMKAKLLAEMPSLG
                     GTGEGLLFAVIDLDMGRGTLTIGISVEFKVEPLLRIRIPVEAFFNFHDSSDWHLYLGK
                     YEDQIQADILSVFEGSGYLMLSGNGISGIQGLPAVTGFSIATGLHVAFIWGSKPARLY
                     AELAAGFDAIVGFDPFRFAGILYVRGSLNLWIISISAWANLNVDIGEDATGQEISRIW
                     GEICGKIDFFFFSISGCVDFAIGDNSVPVPNPPALVRSLKLISRSPALVLGTGTDRPI
                     DGGLGEAKEGAAVPADLPVVPIDSIPALMFSAPPLAPGVEFRSTPLNSTSEAPADGWV
                     LRGDTAFKYTLKAVELSGPLTAGSTPATWWKQKSGDTALEAQLALLSWVPEATPKAIE
                     YSKFLEETVKENWGTVCSPAAPPTPVLWTFLDEILGPSPFGWWLDGRAWPDPPGTVRS
                     MATPLVLRVTEAWRSGDPKLDQMRGVVPASVQGLAVNCLRTAGAPTAGGVASGALSVA
                     ASSALPSAAVGALSSAAVGALSTGAVAVSSAVTALPVLAATAPQRLPIIESARGVRRR
                     DSLPVGNLTITDVLRQLNSGTALSRAALHAVAIAAPNDPATPAGEPKACDSRVLAAPL
                     YDDFMRFRRNPETRRQVELLERLKRLEYRPGPLIDAVVFHTSQIEYATFYLFVHRLAL
                     TEAELVIADANAAGDLFNEHVIRLADTVPGVPYPARWIDAGGPWLPDIYKLVQHSIFL
                     QERGYVAVIVQLKGHPAADRVQIGFRSITPEIEKHLTAVPYYVAAVEGLRRSEVERFD
                     YDQTQQTKKQGAAGGVLGGDTSDYALLEKGTTYSVTVTWDAERERRPAGAPATDHKTI
                     TGQQQTFWFKTDPNPPARLDPWVLMSLPEDNEKHFFSLSGIRLVFATNNVVRLYETYG
                     KRLQVRLKAASFHAPPSTAAVPHPFQLNATTVLPVAKSVLDPFEAALEKVLDGSCVPS
                     SGSRIRHSKVDIPIPLDRYTDYILDVEMVDADAPESAKGPSIYRRAFSTGAFESLIEF
                     AESFFVARVKHRAAPVGALQAIAAKYASAGRSPQGSELDSDFIAAGLEAPELAKEPRL
                     IVFWEGGANPQPAAVLLDASEPLWRSRLIPREIVDPTPAANKRYELLPVPWLEPAEQP
                     GGNAIVQHIVAAPGGQRALISLAPNARGKHLRLALKQVAQKEPYLDGPSATDQFATVA
                     DLILSRAPWEEEE"
     gene            9827..13468
                     /locus_tag="VFQ61_16550"
     CDS             9827..13468
                     /locus_tag="VFQ61_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956120.1"
                     /translation="MAYVVNADQFKAEGAYIDWQVVARRLQIDFGGDARRVLQLRWML
                     HAELGLPTEPFIVWRRRKGAQEFTTLKFEVMPQSFLSGAQKVEWPEGPMLRVRLTIGS
                     TSTGAVIAWAGSPILSNYTAVTLVQTGMTVVEISAPIIDGLLVSPGVVIQDVQGIPLD
                     KLSDAAGWERLEFVGLPVPLPEWQGIARYDAPQGIVTALTSPQKAAVERLERGGPRVG
                     WQPKLPSGETVPPWIAPDALHLVEKELRAGLIDLLEKVLPFTPPNQQRAKKLSVLIPP
                     PENSSGKKMPVEDTTTQVAPLTATLMSGGCDPWLALALGFGTAYPVVLDATGAATAAG
                     SSGLDYDYMITARWEKGLDGNSAPYEMAALVPAPSQAIAPPAPANLYTEMMGHLRPLV
                     PDGDYRTSMKVSWDRPADAELFRPRTYAFMRASVAPAEAVRALMNLRERGGYSYPVIN
                     SALPEPVPPDWWQISDVDRELPIPSNPGTRSLRYGVAQQDIYGQWSVWSVAAGSASQP
                     PVDKVRIVAATLQAALPVPPATSVCKGTLTIEFLWDFRIRRPEKIRFVGRLYAAAHRG
                     DPPPINTPPSGLERSLAHSGSSLDITFLGDVPSAPGATVQLLSEDGERIVTSGADQGA
                     ETRRFRVTVPNFELDFGSTGHIGFALWALGQERIAPQRTGAWSPEPSVIAVSDPRPPK
                     MVPDIVTLASLPDARGECHARLSWNGPGNPSNAVGYFIYESTETKILLANGLSEPPPE
                     KTLSERLTTLYNLFDANPSKREFTRKNDKPISGTSTDITLPRGSSAIHLYLVIGVNAG
                     QVESEWPSGPDAHERLQAIAAPRVMKPAPPTLEAVAFLDTSVSPAVHRARIRVAARPG
                     PRVRKVQLFRVRVDDAARELDTMGPPVATITGSGGDWLVEQQTDIFGANIQRVSGLDS
                     PSGSWKRVWYRAAAWSDADPLRGYLPGRSLESSASWVVIPPATPPDLSGVTVSWPAGG
                     ALGDVLLEWTSQAPLRKTPLGPHVLSVRVNVAGQATPLLDFRGNLEGLPTAAAASGSS
                     VWLTTPGAPAPRGYAAYLRRASEADVLAVTIQLTDPLGRSSERVLRIGAGSVLPAPLI
                     GKVQLRKVLTPAGTQLLFTCNAPLELVETPYVLQVTADRAATVLPLLPRILRPPQRLT
                     VELPIPDVPKDNDANPPAGSEPLLIRRVTSSPNGRSFYAFSRVAVQRFVVRLTAPDGR
                     SAEHIEVVS"
     gene            13472..16828
                     /locus_tag="VFQ61_16555"
     CDS             13472..16828
                     /locus_tag="VFQ61_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956121.1"
                     /translation="MMNIYTLTPAAAQSNLQNDGLDTLGLTTLALSPRWSSAALSGAD
                     GSTRRMPISGSLRAPFRGLLSGLFAPARSTLASNVDANATTLQLASGEGARFPSPTGG
                     GSVRLTLSNDSGSKRETLALTARSGDSLTVVRGFDGSAKQAFSSGDQVRLKVLPSSSS
                     VQFTGAEGQPLTQACAVYRLHPQAVLRLERLMSARYSVPADSAVIRPIPTCIVIHEAE
                     YYLTDQFFDADEVLPVTGSISFHDGRGLILDPIYVACLFEDLLAWLPALKPASVSASV
                     NDTGGIHAAAQSASGNVVHCVSLHGSRLQLPLGTARLIRANSSGTALGDVPADGWLEL
                     GSGERIQAASSDENRLRWGWATNGTLGRAPLTVPTLPAGVSLNRQFFRVAAIDVRWAL
                     LGNRTSAEVLGVQADDQRIPPELLPEVREFVTIDYLPDGPDTLAEGTRVLQRPNLAMI
                     AAVSPELDATLLTPSAPGAAAHWPTFPAAVGSPGATIPAAIPDGAITAAFVAAPSHDV
                     VVTLTGVAPVAAHVRIYPQQFVVIPAIAEEPSFVRGDGGASIAGAGPVEVLLPNPFGL
                     AAGASRPSPAQLVFDLVIAPRVGARRLFGALSVNVQSGPAAVPASPFTGAGPLAALPT
                     TVQGLGPSPLFGVPLPAPPPPGPAPTNPIELAVSLATEPSPRQAPRLPTQARFETLLV
                     TGTTGPGTPAPSGTLLWDAVISGARWARESRSAQHDLGNPGNPAGPDVHAPGVRVTGA
                     LAYDAALVALRRAQPIIPWPTTAGVNPGWVIFTAGDNFDVPDDSANTNNTGIGALLRT
                     IAVGSETPQLSSQTPPAEGLTVQQLLQNAGSALGLSVPPNTISLDNEARIQKEIRREF
                     FISKFGLRDAQWALLRAVSEARELIYIESPQFARTAWSSGAAQSYEIDLAAKLAARLD
                     AAPHLKIIICTPRLSDFAPNFKAFSRQHYVARNEAISLLTAVAPDRVAVFHPVGFPGR
                     TAYVRTTSVIVDDVWSLVGTAHLRRRGMTFDGSVSVASFDRQLQDGYSKKVRDYRRAL
                     MALKLRVPSPTAGQPPAADWIRLSRPDSAFALVRDLLAQSGLGRIQPLWPGPSDTTVL
                     AAQPAAADPEGSTSDQFMVTLAGALNELGD"
     gene            complement(16912..17136)
                     /locus_tag="VFQ61_16560"
     CDS             complement(16912..17136)
                     /locus_tag="VFQ61_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956122.1"
                     /translation="MTANPRIEYLWDVLGSGGIGCFEDRWLGCMISGVLCVDELSSTT
                     CRKSAAVYVKWIAGHLLNAVLVGEAMKTGT"
     gene            complement(17214..18878)
                     /locus_tag="VFQ61_16565"
     CDS             complement(17214..18878)
                     /locus_tag="VFQ61_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956123.1"
                     /translation="MSAPARDCDSVSPFVAPVGLSDSELLSATRRLVGRSNQLLALLL
                     GHLAEVEARGLHRTRACSSLYTYCIYELRFSEDEAYRRVAAARLVRRFPALLDAVASG
                     ELHLTGLLMLGPHLTGENLTEVLARAKHRTKKEITRLVRQLDPLPDVPARIEPLGPAP
                     ARVLSLTPTHGEFIEALCPVRELAPGDRPREWADPVAFTEYGELTEGVGYSDTDSTED
                     AGPTEGAVYSEGARGDSGELSDAVRESIAAGPELGESTQFGESAQFGESVQFGESEQF
                     GESVQFGESVQFGESVQFGGAQAALRVDASHAPARPEAQRYRVEFTASEEYVQLVERA
                     QALLSHSVERVPLSEIQLRAMRALVTELERKERASAKRPDGASAPATAVAIGAAQRLR
                     AEKSARPEHPARPEQPARATQDAPSVSAPNAAPRGAVKPNEAQERADAVPEHTRQHGS
                     SGPAQPRQGCQRGRHVPAAVRRAVFERDQGRCAFIDGSGQRCRETHRLELHHVRAFAV
                     GGEHTLDNLTLRCQAHNALAAEQDFGAHFIQQRRDSRRHECCKQQT"
     gene            19394..20719
                     /locus_tag="VFQ61_16570"
     CDS             19394..20719
                     /locus_tag="VFQ61_16570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HET9956124.1"
                     /translation="MLLRLLPAARLILFGCGFATFGSACLDWSKLENGACGDGFVGRE
                     EACDDGNRISGDGCSDSCRLEPVTCGDGRKAPNEDCDDANSSNDDDCVEGCKQARCGD
                     GHLWDIEEQCDDGNQQDGDGCSMVCSIETTTSGPTCGNGEIDDDEACDDGNVADSDSC
                     LHGCSWATCGDGRVRSGVEECDPGTVDAAPQCTRGCMMCGGMSGSYFRGGNAHCYTLH
                     LDAVTAPQARSNCQAEGGDLWTVTSEGEGNDVIEKLGLGERVWLGLLTNKTGYSWASG
                     EGTKFTNFAAGEPRDTALRCVVLDATSDSRAWSSEACSVKLGYVCERAPALISPADHH
                     AYRLHTASVSAAAARDRCVSDGGHLAALETDAERTFVGKNTGVVAWVDATKSDDQFRW
                     SNGDLVDRALFAAGQPDGMGNEGCLLFNPGDKYADAPCDEVHAFICEFD"
     gene            complement(20726..21505)
                     /locus_tag="VFQ61_16575"
     CDS             complement(20726..21505)
                     /locus_tag="VFQ61_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956125.1"
                     /translation="MPIAPRFRILAFVLSLSAPSIALEPKEEARRLYAEGKAEYAQGH
                     YLQAAELFERAYALSESPALLFNMAQAHRLAGPEHCGIAVRLYRSYLDADASAENRRE
                     VEERIQELGECANQISSTATPAAPTPATRAPSVPAPVTEPTPSPKDFQRPAPRVMPPP
                     PPSWRRPMEPVLVMGAGTTLLVAGGILYGSAWIKHRDAEKHCPCYPGTFSTWEVVTNV
                     SYALLATGAATFGGGLTWWLSASPPSRAQQAQGMLGFGGRF"
     gene            complement(21506..22888)
                     /locus_tag="VFQ61_16580"
     CDS             complement(21506..22888)
                     /locus_tag="VFQ61_16580"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9956126.1"
                     /translation="MQTYAVLDASPCVDDETLFELARGKLTGAALARVEAHLKDCGDC
                     RSVLAEAARSLEPRELVGEALDTVVGLGTVGPGSIARYRIAALLGAGASGVVYRAFDP
                     QLKRAVAVKLLRPELHGSEQSERLLREAQSMARLSDPYVVSVYDVGLHEGSVYLVMEY
                     IDGVTLAQWLAERPRTPNEILSVFNDAGRGLAAAHQKGLVHRDFKPENVLVARDGRAK
                     VTDFGLARESETWASDSNLNEAASTELYAPTRGLVGTPAYMAPELFEGGVASPASDQF
                     AFSTALFTALFDRHPFKAGEGIALGELVTRVRAGTLERPTFEDRLRQRLFLVLRRGLA
                     PNASARYRDLPELLAALERARRVPSRTAQAGLAAVATLLTVLVVARYKTSTVLTHKPE
                     PPRSAVPKSLAQSPPLEHSAPAPSAGSVPSVSAVPSVSAVPRASSSPIQRRHPKPGDV
                     RYKDWLKEPF"
     gene            22918..23718
                     /locus_tag="VFQ61_16585"
     CDS             22918..23718
                     /locus_tag="VFQ61_16585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9956127.1"
                     /translation="MDLACRLDAAVRATPGVRADVAEFVDSVEARLAAAENAERELAE
                     LHIEDMYLAWALGRGDTAAFARFERDFMAQLPLQIQGVTAEAGELEQLVRTRLFVASE
                     GQSARITQYSGRGPFGGWLRMVATRCLLDLKRRRKGAAEPPELDSPEVATDPELDYLK
                     LRHAADFKIVLERALTQLEARQVTLLKLSFIEQLSPSAIGTMYGVSARTVQRWLVELR
                     EALRAATREGLRARLALTPSELESFLGLVDSQLHVSLHRVLGHASSPE"
     gene            23840..>24162
                     /locus_tag="VFQ61_16590"
     CDS             23840..>24162
                     /locus_tag="VFQ61_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956128.1"
                     /translation="MARPNARPKARFSHFRRLLAANSVLALAVACDNTPDKAGSPTSD
                     PPSTQQPPPSSNASGGAEAGGFGGRASGGEGAKQSGGDSSRGGGGEGAVSSCDMQVLM
                     AKPENG"
BASE COUNT         4120 a         7816 c         7860 g         4366 t
ORIGIN      
        1 tgcgcagcgt cgtcgcgatg gggcgaccca tgtagccctc gagcgcgctc tcgaccgcgt
       61 gggttagggc tgcctgccgt agttgggtgc tgaacacggc gttcccgctc tgcacgtagg
      121 tctgaacgtc ggcgcaaccg agcttcgtga gcatcgagcg cagctccgcc atgggcaggc
      181 tgtttttggt gccgacattc actccgcgca gcaaaagggc gtaacgagtc atggctgaag
      241 atttccgagg ccactcatcg tcccgaacct tcgcgcacgc aagaggctct gtagtcgagg
      301 ctccaattag gctcttcgca cggtagtctg cgcgttctcg gtccttacgt agagccgcct
      361 gcgtgagccg cttctaccaa aacgcgagca cgcgcgctgg cgcccccgag gcgccccgaa
      421 ccttgacggc cgcgacgatt gcggtcgctc ctgtcgccgg caccgcttcg agattcgtca
      481 gacactcgag ggcccacttg ttcgctcccg agaggcgctt gtgcactgga tactgggtat
      541 cgcgacccgg atcgaccgac agcgtatcga taccaatgcc cgaaatcgca cgctgatgga
      601 tcaggagatc ggcgacctgc ggagaaaacc caggaaagtg catcacgccg tgcgcgtcca
      661 aattgacgaa tcgacctgac tccgtgactc gcgacgccca tcccgtatgc aaaaacagag
      721 cgcccccgcg cggcacctcg ccgtggactc gctcccaacg aagtagatct gcgacgtcga
      781 acaccgcgtc ttcgtcgcgt gccacgcgct ctgcaatcga gacgatggcc gccggcagca
      841 ccagttttcc taagtcgatc tgctcgatcg aggacgcgcc gtccaaaaag tggcagggtg
      901 catctaagtg cgttccgacg tgctcggtca gctcccaacg attggcgtac acgccatcgt
      961 gggcgttggt ggcgatgggc tggagtcgaa acggaaaggt cttgttctgt accgggatga
     1021 acggaaactc gggcgagaga gtgtgagtga gatctaccca gtgggtaggc cgtggcgctg
     1081 aggctgccgg cggacgagcc caattgtcac aggcggccgt gagcgccgac gcaactcccg
     1141 cagcgagcag acgtcgccgc ccaagcatct cacccaaggt acggcccctt cacttcgccc
     1201 caaccccaac gcggcatggg cgcctccgtc gtcacatcgg cacccggctg agagttgatg
     1261 acagcaagcc gtgagcccca ctccaagtag ccgacgagcg ccgagcggaa ctctggatct
     1321 gacggaaccc ctaagtcatc ggcgcactcg agcagcaagt ctacccagcg tttacgctgg
     1381 cgctccgaaa ggctgcgacc gaggtgtttg cgaatcatat ggggatggcc gccgagctcc
     1441 gcactatacg tgctcggacc gccgaaaacc tcggccagaa aagcggctac atggcgtgcg
     1501 tggctcggtg acatttgggc aaacacgggc ccgagcgtcg cgtcttcacc aacccgcgag
     1561 tagaatcgtt cgaccaagcg ttcgatagcg ggcattccac ccatccactc gaacagagag
     1621 ggtacggagt tggtcaagag ctcaccgatt tgaactgaac cttcaggtgc attgggcgtc
     1681 tccgtctcga atatagagcg accaagcgca tcgtctcatg aatcatccgc agggaagcta
     1741 tcgatcgcat cgatgaccgg cgggggcgcg tccgagcgtc cagcgcattt gaaccttctc
     1801 cgacgtcagc gccgagagtt gcccccaccg tccagtgctc gccgcctgcg gcggctgcgc
     1861 gcttcccgcc tcccccaaac gtcgtgctcg gcgcctgcgg cccctgcgcg cgccgtggag
     1921 ggaggccaac ggcggttcac cgattgaact cctacgtccg cgcgcatgac agtgcctgct
     1981 cggggaagag cctcctgcca ggaccttcag cggcgccagc gaagctgccg agcacgattg
     2041 gtaccaggtg ctcgagaagg acggcctcgt tctccccggg ggtgagcggg acccttgggg
     2101 tcaccagtat gtttacgtca ggcacggaga agatttcgac ttacgtaccg tgggcagaga
     2161 cggcaagtcc ggtaccgcgg acgaccaggt tcgtgcgcag ggctggcgct ggagcacgtg
     2221 cagcgaaccg agtttttgga gctgcggaag gtaggttgat ccattgatgc cttacaggct
     2281 ctgggtcttc gcgttagcgc ctatcccgcc agggcctggc gcagaaggcg caggaggtgc
     2341 tcgatcggtg tggaggcctc tcagcgtggc gcgcggcggc gcatcaccgt cgggtatgac
     2401 gtgttcgcgg cgatagcttt ggagtcacgg gccgttcgtg catgtcgaca ctacgggaag
     2461 ggggtttaga gtgcacaggg cgtctgcctc gggggcagag cgatgtcgtg cctacgaggg
     2521 ggacggcggc tgattcaggc gagcgtaagt tcgaatgcca gtaagtgact atagccgcac
     2581 agtgatgctg acgtgtacaa gaggaaggga cggggaaaag tcggctgtct cgctctcagc
     2641 cgatcaacac ggccgctgac taacaacgcc gccgagtaac aaggagctcg cacgtggctg
     2701 acctatcgct gattgacctg atcacgccct atgtgctgcg tggcggcggc ttcggcgcct
     2761 ggcacccggc tctagctgtt ctcgcggttt acgagcacga ggtggcgctc agcgcggaag
     2821 gcgtcgtgat ccgtggggtg gtgcgcttca cgggggatat tcagccctat atcgatccgg
     2881 atcgtatggt gatcggagtc gagtcctcca atcaggaggg tcacccgctc aatgacccgg
     2941 ggcggcgtag tccgtggatc gatgttcggg acgcaaaaat cgagtttcag ctgacggtgc
     3001 cgagggtggc ctccgcaatt gtggcccagg cggttacgaa tattggagtg gacgcggcgt
     3061 tcgcgggtgc ggccgcggtg ctgaatcaat tcgacatcaa tccgaatgac gcaccgccgt
     3121 cagatttccc gaataccgag ttcacactcg atttcttact tacgaccatc gtactgcgac
     3181 cacccttttt gcgcggtgcg aaacgtgagg cggacgggca gctcgtgccc gatccgagca
     3241 atgaacaagt caagctgacg ctgcccaaga tcaagatgcg tctgatgcag ggttcggccg
     3301 tgggcagccc actcagcgct gtgttgctgt cggcgggcgc gtccgggctg gatgatccgg
     3361 gtgatctctc ggtggccgag ctgatcacga tggacccgcc ctacgcgttc atcggtcctt
     3421 ccaacgtagt tgggtttggc tttcgctcgg ccgtgctgga tctctcgaat ggctcaactc
     3481 caccgcaggt gttggagcag ttcggctatg acgagagctg gacgggcatc tatctgcccg
     3541 agattcggct gttcgtggcg cccaatgggg cgcgtgactt ggcggtggag ggcggcgcgc
     3601 gcaacctgtt gattggtatt ggccaatccg caggagtcac gggtgatttc gatctgcagg
     3661 tgctgaatca aggcgccggt ccgctgaagc tgggcgcgcg gttctacgat gcaacgggtc
     3721 gaagttacgg tttgacgcgg accagcgagt ccgaaggcac ggtgcagttg ccggcgcgga
     3781 cgcgcatgat catcgacgta gatggtgggc gcacccccat tatgacgtcg gcgacgttcg
     3841 acgggggggc cgcccagaac gggcgcgaac acgatatcga cttgtcgtca gcgacgcagc
     3901 gcaacatcgt gctgcaggct acggatacga gcgctccgcc acgggctgca tccatgacgg
     3961 tgcgcgtgtc tcgcctgagt gcccccgcgc tgccgccagc gggttcacaa ccggaggcac
     4021 aaaactccgc cgtcgatctg caaaccacgt cggtgactca gggcggcgtg gccgtgagcg
     4081 cgccacggct gcgcttggta agtgagacgc caaccaccgc tacgattgcg ttggatcgac
     4141 ccggcaaccc cacgtggacg gttggtggtg tgccggcggg tacgagcagc acggtcacgg
     4201 tggacgtcgc accaggagcc acggtcaacg ttcgcgcgga gctccccgga acggccagcg
     4261 caggctcttt taccgcgtac tatcgctttg atcatccaga gcctcaggag ggcagcgact
     4321 acgcgaccaa tcccagcaat tcgcgtacgg agcccgcccc ggacgagggc gcgagcagca
     4381 actggttcgg aggttcagac tcgatcactg cgctgcgcgg cgctctggtc ggcgtgccca
     4441 accacgccgc ggtccgcatc gaggggttcg cgagcttcga gggcccggac actgcgcaga
     4501 cgcgaaccta caataccgcg ttggcgcagc gccgtgccga tgggctgcgc gcgatgatcg
     4561 agcgggtggt cagtgacggg caaactccgg cgctcgcggg gcgcacctac gactttacgc
     4621 cggcgcccgc cgcgaacatg acggcttgga cgacgcaagg aacgtcgctg gccacacgtc
     4681 ggcagtattg gaaggcggtt gcgtcgtggt cgcctgcgcc cgccgcgggc acgattacgg
     4741 agggtacggt ttctcggcgc agcaactccg gaggagggac agatccgatt cccgttcccg
     4801 atccggcgcc tgccagccct ccgcctcagc cgccttcttg gttccggcag ctaggcgcga
     4861 aagtccggat cgtccgcaat caattcgtgg cctgcgaggt gttcggaaag ttcgactttc
     4921 aaactgcctc ggagaaccgg ctcagggaag gggcggggcc gggcacggag ctgccgactt
     4981 gggaggggct cgggagccaa aatccagcgg acgggttggt ggatctgcgc gtcgtggtgc
     5041 agattgacga cgccacggat acggtaaccg tgaccggcta tttcggcgcg gatccagccg
     5101 accgcgacgg gctggcgttg ctcggcacgc ggccaaacca accgccggat cccggaccca
     5161 agaacttcgg gttggatttc ttcggcactt cttgcgtctt cctgcctttg atcgcggaag
     5221 gcgcaggcgc ggtagccaac gaaggcgcgc tcgcggagct cgccgtgacg gcgggtgggc
     5281 ttgggctatg tgcggtgatt gccggcttgg gctggatgcg cacggagcgc atcatttggt
     5341 atggaggtga gctggcggtg gtcgcgaatg cgcgcggtga gtggaccacc acgctgctat
     5401 tcgacatcga gaccgcgatc tcggcggact tcttggggtt ggtgaagatc gcgcgcaacg
     5461 cgccgctcgt ggttcgctac aaggccatcg gcatcctgtt cgggagccct cccgggcaac
     5521 ccgcgtttca gttccggccg atgttcgact cgtccaaagg gtattcgatc gatctttcgc
     5581 ggcccggcgc catcgaagtc gcggagccgc tcggtcagat cctgcagatt ctgggggcgc
     5641 gtatcgcgcg caacaacccc atgatcatcg aggtggatct gggcttcgcc atcgatttgg
     5701 gcgtggtctc gatcgagcgc gcgcgggtgc gcgtgacctt gaatgagtcg ccgagcgtgg
     5761 agcttaccgc gttcggcgcg ggcatcgaca ttcccggtgc cctgcgcggg cgcgggtata
     5821 tggagctcag tgagacggag atcaaaggcc agatcgatct cacgattgtg cccgtttcgg
     5881 tgcgcgtcgc cgccggcgtc ggcgtcgccc agatccccgc cgcgcagggt gggccggcga
     5941 ccggcgtgat cgtgtctctg tatgtcgagt tcccggtggc catccctctc ggtgcctcgg
     6001 ggctcgggat ctacggattt ctcggcctgt tcgcgatgca ctacgcgcgc aatgaagacg
     6061 agatcgacgc gggcaatcag gccccagcgc tggcgtggtt gaaggcaacg ggcggtaacc
     6121 cgacggatct ccagttctgg aagcccaaaa tcaacaactg ggcttttggg gtcggcgcca
     6181 tcctcggcac catgggctcg agcgtgatct tcaatttgaa gggcatgctg atgttggagc
     6241 tgcccgggcc ccgcttgctc ttgatgatga aggccaagct gctggccgag atgccgagct
     6301 tgggcggcac gggcgaaggc ttgttgttcg ccgtgatcga tttggacatg ggtcgcggca
     6361 ccttgacgat cggaatttcg gtcgagttca aggtcgaacc cttgctgcgg atccgcatcc
     6421 ccgtggaggc cttcttcaac ttccatgatt cttcggactg gcacctgtat ctcggtaagt
     6481 acgaagacca gatccaggcc gatatcctga gtgtgttcga gggcagcggc tatttgatgc
     6541 tgtcggggaa cggcatttcc ggcatccaag gcttgccggc cgtgaccggt ttctcgattg
     6601 ccacgggcct gcacgtggcc tttatctggg gctcgaagcc cgctcggctc tacgcggagc
     6661 tcgcggccgg cttcgacgcc atcgtgggtt tcgatccgtt ccggttcgcc ggcattctgt
     6721 atgtgcgcgg ctccctgaat ctctggatta tcagcatctc cgcctgggcg aacctgaacg
     6781 tcgatatcgg ggaggacgcg acgggccaag agatctcgag aatttggggc gagatctgcg
     6841 gcaagatcga cttcttcttc ttcagcatct ccggttgtgt cgactttgcg atcggcgaca
     6901 actcggtgcc ggtgccgaat ccgcctgcgc tcgtgcgctc tctcaagctg atcagccgtt
     6961 cgcccgcgtt ggtgctcggc acgggaaccg atcgcccgat cgacggtggg ctcggcgagg
     7021 caaaagaagg cgccgcggtg cccgccgatt tgcctgtcgt gccgatcgac tcgattcccg
     7081 cgctcatgtt ctccgcgccg ccgctcgcgc ccggggtcga gttccgttcc actccgctga
     7141 acagtacgtc ggaggctccc gcagatggtt gggtcctgcg gggcgacacc gcgttcaagt
     7201 acactctcaa ggctgtggag ctgagtggtc cgctcacggc gggcagcacg ccggccacgt
     7261 ggtggaagca gaagtcgggg gacaccgcgc tcgaggcgca gctcgcgctc ctgagctggg
     7321 tgcccgaagc gactccgaaa gccattgagt acagcaagtt tctggaagag acggtgaaag
     7381 agaactgggg aacggtctgt tctccagcgg ctccccccac gcccgtgctg tggacgttct
     7441 tggatgagat cctgggccct tctccgttcg gctggtggct cgacgggcgc gcctggccgg
     7501 atccgccggg caccgtgcgc tcgatggcga cgcccttggt gctgcgtgtg accgaggcgt
     7561 ggcgctcggg cgatccgaag ctcgatcaga tgcgcggagt ggtgcccgcg tcggtgcagg
     7621 gcctggcggt caattgcctg cgaaccgcgg gggcccctac cgcaggcggt gtagcgtcgg
     7681 gtgcactttc ggttgcggcg tcgagtgcac tgccgagtgc agccgtgggt gcactctcga
     7741 gtgcggccgt gggcgcgtta tcgactgggg cagttgccgt gtcgagcgcc gtgaccgcgc
     7801 tacccgtcct tgccgcgact gcgccgcagc gtctacccat catcgagtcg gcccggggcg
     7861 ttcggcgtag ggatagcttg ccagtcggta acttgacgat cacggatgtg ctgcgccaac
     7921 tcaacagcgg aaccgctctg tcccgcgcgg cgctgcacgc ggtggccatc gccgctccga
     7981 acgacccggc gacgcccgcg ggggagccca aagcttgcga tagccgggtg ctcgcggcgc
     8041 ccttgtacga tgatttcatg cgcttccggc ggaacccgga gacacgacgc caggtcgaac
     8101 tcctcgagcg gctcaagcgc ctcgagtacc gtccaggtcc gttgattgac gcggtggtgt
     8161 tccatacctc gcagatcgag tacgcgacct tctacttgtt cgtgcaccgt ttggcgctca
     8221 ctgaagccga gctggtgatc gccgacgcga acgccgccgg cgatctgttc aatgagcacg
     8281 tgatccgcct cgcggacacc gtaccggggg tgccgtatcc ggcgcgctgg atcgatgctg
     8341 gcggaccctg gctgcccgat atctacaagc tcgtgcagca ctccatcttc cttcaagaac
     8401 gcggttacgt cgcggtcatc gtgcagctga aagggcaccc cgcggctgat cgcgtgcaga
     8461 tcggtttccg gtccatcacc cccgaaatcg agaagcacct caccgccgtt ccgtactacg
     8521 tggctgccgt cgaggggctc cgacgctcgg aagtagagcg cttcgactac gaccaaaccc
     8581 aacagaccaa gaagcaaggc gccgccggcg gagtgctggg cggcgacacc tccgactatg
     8641 cgttgctgga gaagggcacc acgtatagtg tcaccgtgac ctgggacgcc gagcgcgagc
     8701 gccgtcccgc gggcgcgccc gccaccgatc acaagacgat tacggggcag cagcagactt
     8761 tctggttcaa gaccgatccg aacccgcccg cgcgcttgga cccgtgggtc ttgatgagct
     8821 tgcccgaaga caacgagaaa cacttcttct cactctcggg gattcgactg gtcttcgcga
     8881 cgaacaacgt cgtgcgcctc tatgagactt acggcaagcg gctccaggtg cggttgaagg
     8941 cggcttcttt tcacgcgccg ccatcgacgg cggcggtgcc tcatccgttc cagctcaatg
     9001 ccacgacggt gctgccggtg gccaagagcg tgttggatcc gttcgaggcc gccctcgaga
     9061 aggtcttgga tggctcgtgc gtgccgagtt cgggcagtcg aattcgacat tccaaggtgg
     9121 atatccccat tccgctggat cgctatacgg actatatcct cgacgtcgaa atggtcgacg
     9181 ccgacgcgcc cgagagcgcg aaggggccga gcatttaccg gcgcgccttc agcaccggag
     9241 ctttcgagtc actgatcgag ttcgcggagt ccttcttcgt tgctcgggtc aagcatcggg
     9301 ccgctccggt cggtgcactg caggcgatcg cggccaagta cgcgagcgcg gggcggtcac
     9361 cgcaaggcag cgaactcgac tcggacttca tcgcggcggg cctcgaagct cccgaactcg
     9421 ccaaagaacc gcgtttgatc gtgttctggg aggggggcgc caatccacag cctgcggcgg
     9481 tgttgctgga tgcgagcgag ccgctgtggc gttcgaggct catcccccgc gaaatcgtcg
     9541 accctactcc cgccgcgaac aagcgttatg aactgctccc cgtgccctgg ctcgagccgg
     9601 cggagcagcc cggagggaac gccatcgtgc agcacatcgt cgcggcgccg ggcgggcagc
     9661 gagcactgat ttcgctcgcc ccgaacgcgc gcggcaaaca cctgcgcctg gctctgaagc
     9721 aagtggcaca aaaagaaccg tacctcgacg ggccttcggc aactgaccag ttcgcgacgg
     9781 tggcggatct gatcttgagc cgtgctcctt gggaggagga agagtgatgg cttacgtcgt
     9841 caacgcggat cagttcaagg ccgaaggggc ctacatcgat tggcaggtcg tggcgcgacg
     9901 gctgcaaatc gatttcgggg gtgacgcgcg acgggtgctc caactgcgtt ggatgctcca
     9961 cgcggagctc ggcctgccca cagagccgtt catcgtgtgg cgccgccgca agggcgccca
    10021 ggagttcacg acgctgaagt tcgaggtgat gccgcagtca ttcctgtctg gcgctcagaa
    10081 agtggaatgg ccggaagggc ccatgttgcg cgtgcggctc acgatcggct ccacctccac
    10141 gggcgcggtc atcgcctggg ccgggtcacc catcctgtcg aactacacgg ccgtcacgct
    10201 cgtgcagacg ggcatgaccg tggtcgagat cagcgctccg attatcgatg gcttgttggt
    10261 gtcgccgggg gtcgtcattc aagacgtgca aggcatcccg ctcgacaagt tgtcagacgc
    10321 ggcgggctgg gagcgcttgg agttcgtggg tttgccggtg cctctgccgg agtggcaagg
    10381 catcgcccgc tacgacgcgc cccagggcat cgtcacggct ctgaccagcc cgcagaaggc
    10441 ggccgtggag cgcttggaac gtggcgggcc gcgggtgggt tggcagccca agctaccgag
    10501 cggagagacc gttccccctt ggatcgcgcc ggatgctctc cacctcgtgg agaaggaact
    10561 tcgggcgggt ttgatcgacc tgctcgagaa agttctacct ttcaccccgc ccaatcagca
    10621 gcgcgcaaag aagctcagcg tgctgatccc gccgcccgag aactcttcgg gcaagaagat
    10681 gccggtggaa gacacgacca ctcaggtcgc acccctaaca gccacgctca tgagcggtgg
    10741 ctgcgatccg tggctcgcgc tggcgctcgg ctttggcacg gcttatccgg tcgtactcga
    10801 cgcgaccggc gctgcgacgg cggcgggctc ttcggggctc gattacgact acatgatcac
    10861 cgcgcgctgg gagaaaggtt tggacggcaa ttccgcgcct tatgagatgg cggcgctcgt
    10921 cccggctcca agtcaggcga tcgcaccgcc cgcgccggcc aatctctata cagaaatgat
    10981 gggccatcta cggcccctgg ttcccgacgg cgactaccgc acgtccatga aggtgagctg
    11041 ggatcgaccg gcggacgcgg agctgtttcg acctcgcacc tacgctttca tgcgcgcgag
    11101 tgtcgcgccc gcggaggcgg tgcgtgcgtt gatgaacttg cgtgagagag gcggatacag
    11161 ctaccccgtc atcaatagcg cgctgccaga gccggttccg ccggactggt ggcaaatctc
    11221 ggacgtggac cgggagctgc cgattccatc caacccgggc acgcgctcgc tccgctacgg
    11281 agtggcgcaa caagatattt atgggcagtg gagcgtgtgg tccgtcgcgg cgggcagcgc
    11341 gagccagccc cccgtggata aggtgcggat cgtcgcggca acgctccagg cggccttacc
    11401 cgtgccaccg gccacgagtg tgtgcaaagg cacgctgacc attgagtttc tgtgggactt
    11461 ccgtattcgg cgtccggaga agatccgctt cgtgggtcgg ctctacgcgg cggcgcaccg
    11521 aggtgatccg ccgcccatca acaccccgcc gagtgggctc gagcggtcgc ttgcgcacag
    11581 cgggagctcc ctcgatatca cgttcctcgg ggatgttcct tccgcgcccg gcgcgaccgt
    11641 acagttgcta tcggaggacg gggagcggat cgtgacatcc ggcgctgacc agggcgcgga
    11701 aacgcgccgc ttccgggtca cggtgcctaa tttcgaactc gacttcggca gtacgggtca
    11761 cattggcttc gcgctctggg cgctcgggca agagcgcatt gcacctcaac gcaccggcgc
    11821 gtggtcaccc gagccgagcg tgattgcggt ttcggatccg cgaccgccca agatggttcc
    11881 ggatatcgtg acgctcgcgt ccctgccgga tgcgcgcggt gaatgccacg ctcgcttgag
    11941 ctggaatggc ccgggcaatc cctccaacgc cgtcggctat ttcatctatg aatcgaccga
    12001 gacgaaaatc ctcctcgcga atggcctgag tgagccgccg cccgaaaaga cattgagcga
    12061 gcggctgacc acgctttaca acctgtttga cgccaatcct tccaagcgag agttcacacg
    12121 caagaacgac aagcccatct cggggaccag caccgatatt acgctgccgc ggggctcctc
    12181 cgcgatccat ctttacctcg tcatcggtgt caacgcgggc caagtggagt cggagtggcc
    12241 ctccggtccc gacgctcacg agcgtctcca ggcgatcgcg gccccgcgcg tgatgaagcc
    12301 ggctccgccc acgctcgagg ccgtggcgtt cctcgatacg tcggtttctc cggctgtgca
    12361 tcgagcccgc attcgggtgg cagcgcggcc tggtccacgc gtgcggaagg tgcagctgtt
    12421 ccgggtgcgg gtggatgacg ccgcccgcga actcgacacg atggggccgc cggtcgccac
    12481 catcaccggt agcggcggag attggctcgt cgagcaacaa accgacattt tcggagccaa
    12541 cattcagcgc gtatccggct tggattcgcc ctcgggctct tggaagcgag tgtggtaccg
    12601 cgccgcggcg tggtcggacg ccgatccact gcgcggttac ctgccggggc gatctctcga
    12661 gagctcggcc agttgggtcg tgattccccc ggccacgccg cctgatctat cgggggtgac
    12721 ggtgtcttgg cccgcgggag gagcgctggg cgacgtgctg ctcgagtgga cgagccaggc
    12781 ccctctgcgc aagacgccac tcggcccgca cgtgctgtcc gttcgggtca acgtagcggg
    12841 ccaggcgact ccgctgctcg actttcgcgg caatctagag ggcttgccca cggctgcggc
    12901 tgcgagcggt tcgagcgtgt ggctcaccac gcccggggcg ccggcaccgc gcggctatgc
    12961 ggcttatcta cgccgggctt cggaagcgga cgtgttggcg gtgaccattc aactcaccga
    13021 tcctttgggg cgcagcagcg agcgcgtctt gcggattggg gcgggctcgg tgttgcccgc
    13081 tccgctgatc ggcaaggtcc agctccgcaa ggtgctgacc ccggcgggaa ctcagctcct
    13141 gttcacttgt aatgcccctc tcgaactcgt ggagactccc tatgtcctgc aggtcaccgc
    13201 ggatcgggcg gcgacggtgt tgccgctctt accgcgcatc ctgcggccgc cccagcgttt
    13261 gaccgtcgag ctacccattc cggatgttcc gaaggacaac gacgcaaacc cgcctgccgg
    13321 gagtgagccc ctcttgatcc ggcgcgtcac cagctcgccg aacggccgta gcttttacgc
    13381 cttctctcgt gtggccgtgc agcgcttcgt ggtgcgcttg accgcgcccg atgggcgcag
    13441 cgcggaacac atcgaagtgg tgagttgagc catgatgaac atctacacgc tcacgccggc
    13501 tgccgctcaa tccaacctcc agaatgacgg gttggatacg ctggggctca cgacactcgc
    13561 actctctccg cgttggtcga gcgccgcgct ctccggtgca gacgggtcca cccgtcgaat
    13621 gccgatctcg ggctcgctgc gcgccccatt tcgcggcctg ctgagcggcc tgttcgcccc
    13681 cgcgcgctcc acgttggcct cgaacgtcga tgcaaacgcc accacgctgc agctcgcgag
    13741 cggggagggg gcccgctttc cgtccccgac cgggggcgga tcggtgcggc tgaccttgag
    13801 caacgacagc ggctccaagc gcgagacact ggccctcacc gcgcgttcgg gcgactcatt
    13861 gacggtggtc cgcggcttcg atggcagcgc gaaacaggct ttctcgagcg gcgaccaggt
    13921 gaggctcaaa gtgctgccga gctcgagctc ggtccagttc acgggcgccg aggggcaacc
    13981 gctgactcaa gcttgcgcgg tgtaccgcct tcacccgcag gcggtgctgc ggctcgagcg
    14041 cctcatgtct gctcgttatt cggtacccgc agatagcgcg gtgatccgac cgattcctac
    14101 ctgcatcgtg atccacgagg ccgagtacta tttgaccgac cagttcttcg acgcggacga
    14161 agtgctgccg gtgactggtt ctatttcctt tcacgatggc cggggcctca tcctcgatcc
    14221 gatctacgtg gcttgcctgt tcgaggattt gctggcgtgg ctgcccgctc tgaagcccgc
    14281 cagcgtcagc gcctcggtca atgacacggg cggcattcac gcggccgccc agagtgccag
    14341 tggcaacgtc gtccattgcg tctcgctgca cggctctcgt ttgcagctcc cgctcggcac
    14401 ggcccggctc attcgcgcca acagcagcgg caccgcgctt ggggacgtac ccgctgacgg
    14461 ctggctcgaa ctcggcagcg gcgagcgcat ccaggccgcc agcagcgatg agaatcgact
    14521 gcgttggggc tgggcgacga atggcacctt ggggcgggct ccgctcacgg tgccgacgtt
    14581 acccgcggga gtgagcttga atcggcagtt ctttcgggtc gcggccatcg acgtgcgctg
    14641 ggccctgctc ggcaatcgca ccagcgcgga ggtgctcggg gttcaggccg atgatcagcg
    14701 catccccccg gagttgctgc ccgaagtgcg ggagttcgtg accatcgatt acttgccaga
    14761 cggtccggac accctcgcgg agggcacgcg cgtcttgcaa cgtccgaatc tcgctatgat
    14821 cgcggcggtg tcgcccgaac tcgacgccac gctgcttacc ccgagcgcac ccggcgccgc
    14881 ggcgcactgg cccacgttcc ccgctgccgt gggcagcccg ggcgcgacta tcccggcggc
    14941 gattccggat ggcgcaatca ccgccgcctt cgtggctgca ccctctcacg acgtggtggt
    15001 gaccttgacc ggcgtggctc ccgttgcggc ccacgtgcgc atctacccgc aacagttcgt
    15061 ggtgatcccc gccattgccg aagagccttc gttcgtgcgc ggcgacggcg gagctagcat
    15121 cgcgggcgcg ggtcccgtcg aggtgttgct cccgaatcca tttgggctcg cggcgggcgc
    15181 ttcgcgcccg agccccgccc agctcgtgtt cgacctcgtg atcgcgccgc gggttggagc
    15241 tcgcaggctg ttcggtgcgc tcagcgtcaa tgtgcagagc ggccccgccg ccgtgcccgc
    15301 gtcccccttc accggcgccg gcccgctcgc agcgttaccc accaccgtgc agggcctcgg
    15361 gccatcgcct ctgtttggag tgccccttcc agcccctcca ccacccggac ctgcgcccac
    15421 gaatcccatc gaacttgccg tcagcctggc taccgaaccc tcaccgcgcc aagcgccgcg
    15481 tctacccacc caagcccgtt tcgagaccct cctcgtcacg ggaacgaccg gtcctggcac
    15541 gcccgcgccc agcggcaccc tactttggga cgccgtgatc agcggagccc gctgggctcg
    15601 tgagtcccgc tccgcccagc acgacctcgg taaccccggt aaccctgctg gccctgacgt
    15661 gcatgcgccc ggagttcgcg tcacgggcgc cttggcctac gacgccgcgc tcgtcgcctt
    15721 gcggcgcgcc cagcccatca tcccgtggcc taccaccgcg ggcgtgaacc ccggttgggt
    15781 gatcttcacc gctggagaca acttcgatgt tcccgacgac tctgccaaca cgaacaacac
    15841 cggcattggc gcgctcctcc gcaccatcgc cgtcggcagc gaaactccgc agctgagcag
    15901 ccaaacaccg cccgcggagg gcctgaccgt gcagcaactg ctccaaaacg ccggtagcgc
    15961 cctcggcctc agtgtgcccc cgaacacgat cagcctcgac aacgaagcgc gcatccaaaa
    16021 agagattcgg cgcgaattct tcatctccaa gttcgggctc cgcgacgcgc aatgggccct
    16081 gctgcgagcc gtctccgaag cccgtgagct catctacatc gaatcccctc agtttgcccg
    16141 cactgcctgg tcgagcggag ctgcccagtc ttacgagatc gatctcgcgg ccaagctcgc
    16201 ggcccgcctt gacgcggccc ctcacttgaa aatcattatt tgcactccgc ggctcagcga
    16261 cttcgcccca aacttcaagg ccttctctcg gcagcactac gtcgctcgca acgaagcgat
    16321 tagcctcctc accgcggtcg ctccagaccg cgttgccgtg tttcaccccg tcggctttcc
    16381 cgggcgaact gcctacgtgc gcaccacctc cgtgatcgtc gacgacgtct ggagtttggt
    16441 cggaacggct cacctgcgtc gtcgcggcat gaccttcgac ggcagcgtct ccgtcgcttc
    16501 cttcgatcgg cagctccagg acggctactc gaagaaggta cgcgactatc ggcgcgccct
    16561 catggcgttg aagctgcgcg tcccgagccc caccgccggc cagcctcccg ccgccgattg
    16621 gatccgccta tcccgccccg actccgcctt cgcgctcgtc cgtgacttgc tcgcgcaaag
    16681 cggcctaggg cgcattcagc ccctttggcc tggcccctcg gacaccaccg tcttggcggc
    16741 ccaacccgcc gctgccgatc ccgaaggctc caccagcgac caattcatgg tcactctggc
    16801 gggcgcactc aacgaactcg gagattgaga gcgaacggct cgagacaccc acgagctgga
    16861 gctggggttg gcgttggaat ccgccttcct cttgtttgtg cgtcaaaggg gtcaagttcc
    16921 ggtcttcatc gcctctccca ccaaaaccgc attcaagagg tgaccggcga tccatttcac
    16981 gtataccgca gccgatttcc tgcacgtcgt gctcgacaac tcatcgacgc acagcacgcc
    17041 ggaaatcatg catcccaacc atcggtcctc gaaacatcct atgcctccgc tgccgaggac
    17101 atcccaaagg tactcgatgc gcggattggc tgtcactgat ggatggcgag cggtggccga
    17161 cgctttaatc gactggctta gtaactaaca attccggcgg gcgatcttct ctgtcatgtc
    17221 tgctgtttgc aacattcgtg tctgcgcgag tcgcgacgct gctggatgaa gtgcgcaccg
    17281 aaatcctgct cggcggcgag tgcgttatgt gcctggcagc gcagcgtgag attgtccaat
    17341 gtgtgctcgc cgcccaccgc aaacgctctc acgtgatgga gctcgagccg atgcgtctcg
    17401 cggcaacgct gccctgaacc atcgatgaac gcgcagcgcc cctggtcgcg ctcgaagacg
    17461 gcacgccgaa cggccgcggg cacgtgtcgc ccgcgttggc aaccctggcg cggctgagcc
    17521 ggcccactac tcccgtgctg gcgcgtgtgc tccggcacgg cgtcagcacg ctcctgagcc
    17581 tcgttcggtt tgacagcacc tcgcggggcc gcgtttggcg ccgaaaccga gggcgcatcc
    17641 tgcgttgctc gggccggttg ttctggacgc gctgggtgtt ccggacgggc tgacttttcc
    17701 gctcgaagcc tttgcgcggc gccgattgct accgcggttg ccggcgcgct cgcgccgtcc
    17761 ggccgcttcg ctgaagcgcg ctcttttctt tcgagctcag tcacgagcgc gcgcatcgcg
    17821 cggagctgga tctcactaag gggaacgcgc tccacagagt gggagagcag cgcctgtgcc
    17881 ctctcgacga gctggacgta ctcctcactc gcggtgaact ccacgcgata ccgctgagcc
    17941 tccggtcgcg ctggcgcgtg tgaagcgtcc acacgcagtg ccgcctgcgc accaccgaac
    18001 tgtacggact caccgaactg tacggactca ccgaactgta cggactcacc gaactgttcg
    18061 gactcaccga actgtacgga ctcgccgaac tgtgcggact cgccgaactg tgtggactcg
    18121 ccgagctcgg gtcccgcggc gatgctctcg cgcaccgcat ccgagagttc acctgaatct
    18181 ccccgcgcgc cttcactgta cactgcgccc tcggtgggcc ccgcatcttc cgttgaatcg
    18241 gtgtcgctgt acccgacccc ctcggtcagc tcgccgtatt cggtgaacgc cacgggatcc
    18301 gcccactcgc gaggcctgtc gcctggggca agctcgcgaa ccggacacag cgcctcgatg
    18361 aactcgccat gggtcggcgt cagcgagagt acccgcgccg gcgcgggacc gaggggctcg
    18421 atgcgcgctg gaacgtcggg cagcgggtcg agttgccgaa ccaggcgcgt gatctccttc
    18481 tttgtccgat gcttggctcg ggccagcacc tcggtcaggt tctcgccagt tagatgcgga
    18541 ccgagcatca acaagccggt gaggtggagc tcgccggagg cgaccgcgtc gagcaaagcc
    18601 gggaagcggc gaaccaggcg cgccgctgct actcgtcggt aggcttcgtc ttctgagaat
    18661 cgcagctcat aaatacaata cgtatagaga ctcgagcagg cgcgagtgcg gtgaagcccg
    18721 cgcgcctcca cctcggcgag gtgaccgagc agcagcgcga gcagctgatt ggagcgaccc
    18781 accaggcgtc gcgttgcact gaggagctcg ctgtcagaca gcccgacggg cgcgacgaac
    18841 ggagacactg aatcgcaatc gcgggccggt gcgctcatga acagcacctt acaccctggt
    18901 tttcggacct ccctattcgc gctctcgcgc gtcttcacac gcagacacca cgttcgagcc
    18961 gactgcctgc acgaaagcag cacgatgggt caagcacgcg cggcaaacgc gagaaaatcc
    19021 gtgtcgccgc tgcgagcggc gtcggcgcgc gccaatcgtg tcaagcatcg aatgcggagt
    19081 ctgcgtgaca tggcccaaca ccgccgtgag cgcttcgtga tcacatcagc tcgaacggat
    19141 tgaaagcaac gtccttcgct ttccgtagtc gagatgccgc cgccagtgct tcgtcggcgc
    19201 tcgatgggac gcacgggatc tgcgtggtgg cgcggcttcg gcggcgccga ggataccggc
    19261 gtcggcgtag gcgggctggt acacgccgca gcagtgaggg caccgtgcgt cacgcgaact
    19321 caccctgcgt agctctctct ccaaattgcg caccctcttg gccagcgggc aaagggagga
    19381 tacggttcgt tcggtgctgc ttcggttact ccccgccgct cggttgattt tgttcggttg
    19441 tgggtttgca acgttcggat cggcgtgcct cgattggagc aagctcgaaa acggcgcttg
    19501 tggcgacggg ttcgtgggac gcgaggaggc ctgtgatgat ggcaatcgca tctccggaga
    19561 tggctgtagc gacagctgtc gtctcgaacc ggtgacgtgc ggagatggcc gcaaagcacc
    19621 aaatgaggac tgcgacgacg ccaattcctc gaacgacgac gactgtgtcg agggttgcaa
    19681 acaggctcgc tgcggtgacg ggcacctgtg ggatatcgag gagcagtgcg acgacggcaa
    19741 tcagcaagac ggcgacggct gttcgatggt ttgctcgatc gagaccacga cgagcgggcc
    19801 gacctgcggc aacggcgaga ttgacgacga cgaggcctgc gacgacggca atgtggcgga
    19861 ctccgattct tgtctccacg gctgtagttg ggccacttgc ggcgacggaa gggttcggag
    19921 cggcgtagaa gagtgcgacc ccggaaccgt cgatgccgct ccgcagtgca cgcgcgggtg
    19981 catgatgtgc ggcggcatga gcggatcata ctttcggggc gggaatgctc attgctacac
    20041 gcttcatctc gacgcagtca cggcaccgca agcgcggagc aactgccagg ctgaaggagg
    20101 ggacctgtgg acggtcacgt ccgaaggcga gggtaacgac gtgatcgaga agctaggctt
    20161 gggagaacga gtttggctgg ggctactgac gaacaagacc ggctacagct gggccagtgg
    20221 agagggcacc aaattcacca actttgccgc cggcgagccg cgcgacacgg cgctccgctg
    20281 cgtggtgctc gatgcgacga gcgatagccg cgcgtggagc tcggaggcgt gttcggtcaa
    20341 gctcggttac gtatgcgaac gcgctccggc gcttatttcg cccgccgacc atcacgccta
    20401 tcggctgcat accgcgagcg tcagcgccgc agcggcgcgt gaccgctgcg tgagtgacgg
    20461 agggcatctg gccgcgctcg aaaccgatgc cgaacgcacg ttcgtcggca aaaatacagg
    20521 cgtggtcgca tgggtcgacg cgacgaagag cgatgatcaa ttcaggtggt ccaacggtga
    20581 cttggtggat cgagcgctat tcgctgccgg tcaacctgat gggatgggca acgagggctg
    20641 tcttctcttc aaccccggcg ataagtatgc cgacgctcct tgtgacgaag tccacgcctt
    20701 tatttgtgag ttcgactaag ttgcttcaaa agcgtccgcc gaagccgagc atgccctggg
    20761 cctgctgagc gcggctcggt gggctcgcgc tgagccacca ggtgagccca cccccaaacg
    20821 tggcggcacc ggtggccaag agcgcgtagc tgacgttcgt gaccacctcc cacgtcgaga
    20881 acgtaccggg gtagcaaggg cagtgcttct cggcatcgcg gtgcttgatc caggcgcttc
    20941 catagagaat gccgcctgca acgagcagcg tggtacccgc acccatcacc agtacgggct
    21001 ccatgggtct tctccaactt ggcggcggtg gaggcatgac tcgaggcgct ggacgctgaa
    21061 aatccttggg ggagggggtg ggctcagtca ccggcgcggg aacggacgga gcacgcgtcg
    21121 cgggtgtcgg ggctgcaggc gttgcagtgg acgagatctg gttggcgcat tcaccgagct
    21181 cctgaatgcg ctcctccacc tcgcgtcgat tctcggcgct cgcatctgca tcgaggtagc
    21241 tgcgataaag acgcacggcg atcccgcaat gttcgggccc cgccaagcgg tgcgcctgag
    21301 ccatgttgaa gagcagggca ggggattcgg agagcgcgta agcgcgctcg aacaactccg
    21361 ccgcttgcag ataatgacct tgtgcgtatt ccgccttgcc ttcggcgtac agtctgcgcg
    21421 cctcctcctt tggctcgagt gcgatcgatg gcgcgctcag cgacagcaca aaggccagga
    21481 tccggaagcg aggggcaatc ggcattcaga acggctcttt cagccaatcc ttgtagcgca
    21541 catcgccggg cttcgggtgg cggcgctgga tcggcgagga ggaagctctt ggcactgccg
    21601 agaccgatgg cactgccgag accgatggca cgctccccgc gctaggggca ggagccgaat
    21661 gctcgagcgg agggctttgc gccaggcttt tcggcacggc ggagcgcggc ggttctggtt
    21721 tgtgtgtcaa aaccgtcgat gttttgtagc gagcgacgac cagtaccgtc agcaaagtcg
    21781 cgactgcggc gagaccagcc tgcgcagtgc gcgaagggac gcgccgagct ctttcgagcg
    21841 ccgcaaggag ctccggtaga tcgcgatagc gcgccgacgc attgggagcg agtccccgcc
    21901 tcagcacgag aaacaagcgt tgcctcaggc ggtcctcgaa cgtcggtcgt tcgagcgtgc
    21961 ccgctcgcac tcgcgtgacg agctctccga gtgcaatacc ctctcccgcc ttgaacgggt
    22021 gtctatcgaa cagcgcggtg aacagggctg tcgagaatgc aaattgatca ctcgcagggc
    22081 tggcgacgcc gccctcgaac aattcgggcg ccatgtaagc gggagtgccg accagcccgc
    22141 gcgtgggcgc atacagctcg gtgctcgcgg cctcgttgag attcgagtcg gaagcccagg
    22201 tttcactctc gcgcgctaac ccgaagtcgg tcaccttggc tcgaccatcg cgcgccacga
    22261 gcacgttctc tggcttgaag tcgcgatgaa ccaagccttt ttggtgcgct gccgcgaggc
    22321 cgcgacccgc gtcattgaac acactcaaga tctcgttcgg tgtgcggggg cgctcggcga
    22381 gccattgggc gagcgtcaca ccgtcgatgt actccatcac caaatacacg ctgccttcgt
    22441 gcaaccccac gtcgtagacc gacacaacgt agggatccga caggcgcgcc atcgactgag
    22501 cctcacgcag caggcgctcg gactgctccg agccatgcag ctctgggcgc aatagcttca
    22561 ccgcgaccgc gcgcttcagc tgggggtcga aggcgcgata cactacgccg gacgcgccag
    22621 caccgagcag cgccgcaatg cggtagcgcg cgatgctgcc tggccccacg gtaccgagac
    22681 ccacgacggt atcgagagct tcgccgacga gctcacgagg ttccagcgat cgagcggcct
    22741 ctgccagcac cgagcggcag tctccacaat ccttcaagtg agcttccacc ctcgcgagcg
    22801 ccgctccggt gagcttgcct cgcgcgagct cgaacagcgt ttcgtcgtcc acgcaggggc
    22861 tcgcgtctag cacagcgtag gtttgcattc tccggtgcgg ccgatcaaga tggccctatg
    22921 gacctcgcgt gccgcctcga tgctgccgtg cgagcgacgc cgggtgtgcg cgcggatgta
    22981 gcagagttcg tggacagcgt cgaagcgcgg ctggcggcag cggagaacgc cgaacgagaa
    23041 ctcgcggagc tccacatcga ggacatgtac cttgcctggg cgctggggcg gggcgacacg
    23101 gcggcattcg cgcgcttcga gcgagacttc atggctcagc taccgctcca gatccaaggc
    23161 gtgaccgccg aagccgggga gctcgagcag ctggtccgca cccgcttgtt cgttgcgagc
    23221 gagggccaaa gcgcgcggat tactcagtac tcgggccggg gccccttcgg cgggtggctg
    23281 cgcatggtgg cgacacgctg cctgctggat ctgaaacgga gacgaaaggg cgccgcggag
    23341 ccgcccgagc tcgatagccc cgaggtggct accgatccgg agctcgacta cctgaagctg
    23401 cgccacgcgg cggacttcaa gatcgtgctc gagcgagcgc tgacccaact cgaggcacga
    23461 caggtcacct tgctgaaact ctcgttcatc gaacaactgt cgccctcggc cataggcacg
    23521 atgtatggag tctctgcgcg aactgtccag cgctggttgg tcgagctacg cgaggcgctg
    23581 cgcgccgcga cgcgtgaagg cctgcgcgct cggctcgccc tcaccccgtc cgagctcgaa
    23641 agtttcctag gactggtcga cagccagctt cacgtgagcc tacatcgggt actcggccac
    23701 gcctcgtctc cggagtagcc ggatcctcga acccctcggc tgattcgtca tcggcgccaa
    23761 atcgaggtgg ttcgatcttt atttcgattt ctgtgtcgcg atccgcgggc ggcttcgtca
    23821 acgagtcgag gaatccaact tggcccgccc aaacgcccgc cccaaagccc gcttcagcca
    23881 cttccggcgg ctattggccg caaactcggt actggcgctc gccgtagcct gcgacaacac
    23941 tccagataaa gccggctcgc ccacttcgga cccaccctcc actcagcaac cgccgccgag
    24001 ttcgaatgct tccggcggcg ccgaagccgg cggcttcggc ggccgcgcga gcggcggcga
    24061 aggcgccaaa cagagcggcg gcgatagctc acgagggggc ggcggcgaag gtgcggtcag
    24121 cagttgtgac atgcaggtct tgatggcgaa gccagaaaac gg
//
LOCUS       DASTKU010000480         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_759364, whole genome shotgun sequence.
ACCESSION   DASTKU010000480 DASTKU010000000
VERSION     DASTKU010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759364"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..97
                     /locus_tag="VFQ61_16595"
     CDS             32..97
                     /locus_tag="VFQ61_16595"
                     /inference="COORDINATES: protein motif:HMM:NF012392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956129.1"
                     /translation="MSRTFRKTFGVTPSTLRPSKS"
     gene            144..548
                     /locus_tag="VFQ61_16600"
     CDS             144..548
                     /locus_tag="VFQ61_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956130.1"
                     /translation="MTMLAKRIDSIASGLLANQFWSYGSTHQERLNLLLHALTVPLFL
                     LGSAAVPIGLIVGRADVLPPLGMLAMFTALLAQARGHRREARAPASFRGPLDFVARIF
                     AEQWLTFPRFVLSGGFHRAWRAARPGVHESSP"
     gene            complement(594..1592)
                     /locus_tag="VFQ61_16605"
     CDS             complement(594..1592)
                     /locus_tag="VFQ61_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956131.1"
                     /translation="MRQRRGEPWPARLSPMLALGLLACSEVLDAGSTRPHGTLPVDER
                     NPIIITNDNVGDNWQGEYALLLSNGGGPKLAGILINTSGPWPELDANVEGWRGLVEAA
                     RASGLEAPDPTTSVGERLVRPGSGRIEDTEPNRSEGALFIIEASRRLSLPYRPLVIVT
                     GGRLTDVADAYLVDPTVADRIVVVSSLGKTSDSGAVMGPPNGEMDPWAGAIVTARLKY
                     VQVSSFYDQLMDVPASRVSELPDNAFGAWIADKRARIWNLPEASDQVAVAAVGITGFA
                     LKVERISATSLAANSGQGPDLTRDPDGPAWLVSDVEGTEATAGFWRLLSELRAVPP"
     gene            complement(1612..2325)
                     /locus_tag="VFQ61_16610"
     CDS             complement(1612..2325)
                     /locus_tag="VFQ61_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956132.1"
                     /translation="MSRASGSEARLRELLLRARVPPRLPEAVRARALTRARKTVSAPF
                     YVLATPIPPPARFTRHALIAAALVLGMGGVAFALNARWKHQSAPAVASPVAGSLPAKA
                     VSSETVPLAHAPTIPSAQQPPSRVEDPNQDGARASRPESSLKKYGAERELMHRAHSAF
                     AEGDFGATLKLLAEHARRFPRGQLAEEREALRVRSLASAGRIAEARRAAQAFADHFPR
                     SVLLARIQQIAGGKADRDR"
     gene            complement(2318..>2601)
                     /locus_tag="VFQ61_16615"
     CDS             complement(2318..>2601)
                     /locus_tag="VFQ61_16615"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET9956133.1"
                     /translation="TPYASTDVEDGTADPERDLQSRQSSALLSSALECVPFKRRAVVI
                     MYELDGLSIVEIARTLSISRFGAYARLHKGRSELANAVRRLQKKRSRDV"
BASE COUNT          467 a          866 c          805 g          463 t
ORIGIN      
        1 ggccgaagcc ggcttcgccg acgcggcgca catgagccgc acgttccgga aaacgtttgg
       61 ggttacgccc tcgacactac ggccgtccaa gtcgtagcca gctgcttcaa gcccgggaga
      121 ggctcgagac acatggttga agcatgacca tgctagctaa gcgcatcgat tcgattgcga
      181 gtggattgtt ggcgaaccag ttctggagct atgggtcgac ccaccaggaa cgattgaacc
      241 tgctgttgca tgctttgacc gtcccgctct ttctactcgg ctcagcggca gtcccgattg
      301 gcttaatcgt tggccgtgcg gacgtcctgc cgccgcttgg catgctcgcg atgttcacgg
      361 cgctgctggc tcaagcacgg ggccatcgtc gagaagcccg tgcaccggcc agcttccgcg
      421 gcccactcga cttcgttgcc cgcatcttcg cagagcagtg gctcaccttc cctcgcttcg
      481 tcctgagcgg cggatttcat cgcgcctggc gcgcggcaag gcccggcgtg cacgagtcga
      541 gcccatgact gggccctatg actcgacccc atgactcgac ccctgcgtcg cgactagggc
      601 ggaacagccc gcagctcgga gagcaagcgc cagaagccag ccgtcgcttc ggttccttcg
      661 acgtcgctga ccagccacgc cggcccatcg ggatcgcgtg tgaggtctgg accttgccca
      721 gaattcgcgg ccagtgatgt agcggagatg cgctccactt tcagcgcgaa gcccgtgatg
      781 cccaccgcgg ctaccgcaac ctgatccgaa gcttctggca gattccagat gcgagcgcgc
      841 ttgtccgcga tccaggcgcc gaacgcgttg tcaggcagtt ccgagacgcg cgacgcgggc
      901 acgtccatca gctgatcgta aaagctgctc acctgcacgt acttcaaacg agccgtgacg
      961 atcgcgccgg cccaaggatc catttccccg ttggggggtc ccatgaccgc gccggagtcg
     1021 gaggtctttc cgagcgaaga cacgaccacg attctatccg ccacggtcgg gtcaaccaga
     1081 taggcgtccg ccacgtccgt aagccgcccg ccggtcacga tcaccagagg ccggtaaggg
     1141 aggctgagcc gacgcgaagc ctcgatgatg aatagtgcgc cttcagaacg attaggctcg
     1201 gtgtcctcga tccgaccgct tccgggcctg acgagtcgtt cgccgacgct ggtggtggga
     1261 tcgggagctt cgagcccgct cgcgcgcgcg gcctcgacga gcccacgcca gccttcgaca
     1321 ttcgcgtcca actcgggcca ggggccgctg gtgttgatca gaatgcccgc gagcttgggc
     1381 ccaccgccat tcgagagcag caatgcatac tcgccctgcc aattgtcccc gacattgtca
     1441 ttcgtgatga tgattggatt gcgctcatca accggcaggg tgccgtgcgg gcgggtggaa
     1501 ccggcgtcca gcacctcgct acaagccaag agcccgagcg caagcatcgg gctcagtcgt
     1561 gcgggccaag gctcacctct gcgctgtctc acgttcccga gcccccgcgc atcaccgatc
     1621 tcggtcggct ttaccaccag caatttgttg aattcgcgcg agcaggacgc tgcgtggaaa
     1681 gtgatctgca aaggcctggg cggcgcggcg cgcctccgcg attcggcccg cgctcgccag
     1741 cgaacgcacg cgcaacgcct ctcgctcttc ggcgagctga ccacggggga acctccgcgc
     1801 gtgctccgcc aaaagcttga gcgttgcccc gaagtccccc tccgcaaaag cagagtgcgc
     1861 tcggtgcatg agctcgcgct ctgcgccata cttcttcaga gacgattcgg gacgcgaagc
     1921 gcgcgccccg tcctgattcg ggtcctctac ccgggaaggc ggttgctgtg cagacgggat
     1981 cgtaggggca tgggcgagcg ggaccgtttc cgacgaaacc gcttttgccg gtagtgaacc
     2041 tgcaacgggg ctagcgacag caggcgcact ctggtgtttc cagcgcgcgt tgagcgcgaa
     2101 ggccacaccg cccatgccta aaaccagcgc cgccgcgatc agagcatggc gggtaaagcg
     2161 cgccggtgga ggaattggcg tagccagaac gtaaaagggc gcgctcacgg tttttcgcgc
     2221 tcgcgtgagc gctcgtgcgc gaaccgcctc cggcaggcgc ggcggaacgc gcgcgcgaag
     2281 caacagctcc cgcaagcgag cttcggagcc gctcgcccta gacatcgcgg ctccgctttt
     2341 tttgaagtcg ccgcactgca ttcgccagct cgcttcggcc cttgtgcagc cgagcgtagg
     2401 ccccgaaccg actaatgctg agggtacgcg cgatctcaac gattgagagt ccgtcgagct
     2461 cgtacatgat gactaccgct cgccgcttga agggcacaca ctcgagcgcc gaggacaaca
     2521 atgccgacga ttgccgagat tgcagatctc gttccggatc cgcagtcccg tcttccacat
     2581 ctgtagatgc ataaggagtc t
//
LOCUS       DASTKU010000481        20536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1430706, whole genome shotgun sequence.
ACCESSION   DASTKU010000481 DASTKU010000000
VERSION     DASTKU010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 20536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20536
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430706"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFQ61_16620"
     CDS             <1..460
                     /locus_tag="VFQ61_16620"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9956134.1"
                     /translation="AVPGLRLGYCVLPPSWAERLEAERPPWSVNAAAQAAVRVACDPS
                     VTSFVQRSKERLRSDRIFLHEQLEQLGLRVHTSVAPYCLAKLPEPLTATVVRSELLEQ
                     HRVLVRDATSFCLPGHVRVAARPPEQALRLVSALRSTLGRSTLGRTRHGS"
     gene            574..801
                     /locus_tag="VFQ61_16625"
     CDS             574..801
                     /locus_tag="VFQ61_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956135.1"
                     /translation="MFSSGAPSLGLGMFALVSGLFCTACPEKKEPAADPEPKAAAAEP
                     KAAAPTPGAADKDDDDAKPPEREEKKDQGGW"
     gene            858..3425
                     /locus_tag="VFQ61_16630"
     CDS             858..3425
                     /locus_tag="VFQ61_16630"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9956136.1"
                     /translation="MARKRYVVIGEGAAGLTAVEELRRLDAHAVIGLFSDEPYPAYFR
                     AALTNYLLGELREDQLFVQAPDFFQAARIRRALTRVVAIDPKRGLVWDAGSAQPTPFD
                     ALLVASGAYARPPTFEGGALPGVVTLRTLYDARVVMEWLRLGSLKSAVILGAGPLGLE
                     WAHALLEHRIQVTIIERGTRLLASALDEVASDLLAARLRQAGVQVIFADEVVRAYAGP
                     SGSVAGVVLRSGQQLACGLVAAALGVVPNTGFLSSSGLKLTHSGALAVDATLRSSEPN
                     VWGAGDVVSVNGQSLALWEPAKLQGRLAARNMVGGRMQYEPGTHYFATRLFDLDFARL
                     GSIDAAPTRDVLVDLPRGTGKIAYRKLVFEQGKLIGALMMGERSERVRALGRTLKRLI
                     DQRIDVSSIRDALLTPEFDLSGWLDRQKRVAQPTPGQKTQSEIPLEAKLRGTQGLALS
                     QLTQHVAGLTPGAHSGGNATGAMAMPTPSGGAPFSVFASPSNASSGAPVGQGPAREAP
                     RATRMLSIGLPAELAQAQPKDVPSRKAYLEYRGSRRPIERRVVRLGADAHCELLLDAA
                     GVEGMHAELCEYAGSFYVQDLGTRAGTWLNARPVTSAERLRTGDRLRIGAAELVFQVE
                     SEPKEERSDGATPPVPRGDLVAAPRLLVQSGAKIGHLVRLAQRPCLIGRSPACDLSLN
                     EPFVAPEHARVFAQGSEFFLEPVENASALDPRFGTWLQDSPLYPGQRQLLTENSRIRI
                     GSVELAFTRAALPDAAGALMPGGRVVVDSGPDLGKTQSVGPGLRIGSGPEANFVLQGL
                     PALVLEIAPHEREFFARDLSRGHALRAGRSLGDAWVALSHGEQLLLGGSVLLRFEEV"
     gene            3422..5284
                     /locus_tag="VFQ61_16635"
     CDS             3422..5284
                     /locus_tag="VFQ61_16635"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9956137.1"
                     /translation="MTIGAPAAQELSPYETELARRVEAQAAACIGCNDCLLACPISES
                     ANVTIGELNRALFLPVIQERRVIEFVSACTQCNQCVPACPADLSRAEMVLFNKLKVED
                     TVADYELSLSARTVTFPSGMRLDELARELGKLELFRGASFLALRRLIQKSTLRLLVPG
                     ETLCSEGEYHERLSVVLKGGLGQYSRGARGESTHLLTLGPGSFLGELSVLGGAPEPHT
                     IIAQTTSVVLDAPKAAVLRVMAQAPDVRHALESLYAKRALYTYAKSPGALGALPDEAI
                     RYLLENASLEFMASGQRLFEIGEPPQALFLVRSGFLRAVQRDAQGERVLTYFREGDVF
                     GLLGLLERDAGQRYAAHAASRAEVIRIPSAVLFAVLAHHPTAQAALARAAYQAEQLAR
                     SGEIGLGPVPQAARARSLSGSPEPSLPGERERLGASVLVERGLASGREVLVIDQTRCT
                     ACGNCIDSCERRHGASRLSLRGIQVDQYLFPSACRHCADPACLLCSVNGIVRLPSGEI
                     QIVRDTCIGCGACAERCPYGTISMHAAQTPKPSLWASLLDFLAGSTREESVRVASPDE
                     PRVAVKCDLCAGHADYACVSACPTAAAFRVNPDRVLGEGEGSNRSASSPRRGKP"
     gene            5281..7626
                     /locus_tag="VFQ61_16640"
     CDS             5281..7626
                     /locus_tag="VFQ61_16640"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET9956138.1"
                     /translation="MTGPLLQRRKTWLERAWIWLLFGSAAAGVSGAAALFIYSGGLSD
                     FEIFPAVARGYTPLGLTYGVLATVCLAVTFFYSFRRRPLQEALPLGKGSMAAWLWVHV
                     VFGTLALWFALLHAGAGNLALRASSGKALLILLALIALGGALWRLLYLLLPKRATREV
                     GNYATEESRARSDAIAIELEKLAAGRSPTLQRLTSQLFEAPLTLSEVASLGATLPPQE
                     RAVFTEIARLTAERHASLGREAKQRRYVARLQGMRLYHVPISLLLAIGLPLHVVLAFD
                     LPERLLANTRAAPLVASFEPAQTCAQCHARVVEEWRSSMHAHALSGPIMIAQTNLAAR
                     TTLQAGPTPDPKQICVNCHGPLAARLSPSDKLPVPALQTTTLTPWLDAQNSLLTDGVN
                     CIACHAADGEPHTGGAALTAFQSTLAAGRTYGGALDQPVGNAFHRSVDSSHASAPERL
                     CQSCHSVVYDRNGDGKIEKGPDLVLQDLFSEWQAYRASGGAHCVDCHMPLLGKGRAAE
                     SARIPIEQDDEAPPRPLRSHRFIGPDYPLDEPALRDAHRPARTALLQQAAKLSVVAGS
                     VSFVDGRAQVRLAVANTGVGHNLPGGFAFVRQMWIELQVLDSAGRVAAQTGTLRRSSD
                     DLCDAELLRDANPLRGFAVGCLKPDESLVNFQQLLLDRIEFTRDGGGRAVVTRPPGAV
                     EVVVQHLTSGSVPRVRPFDRRPTPPLSPGESRTFDFSLELPSGTTPAQLRARLLFRAV
                     PPYFLRALEAQQQPEDGPPLSRWIDQIEIVEMASVTAAFNR"
     gene            complement(7642..8580)
                     /locus_tag="VFQ61_16645"
     CDS             complement(7642..8580)
                     /locus_tag="VFQ61_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956139.1"
                     /translation="MSHPSQRYPADPAPSSVLPTPPPPPAPPPLRRRNAAGPSSEWPA
                     AQVAPEAPMPSFAEDGVPYTYEEPELVIPLVPKGAAGDGSSEAEFTRVDGRLARMKQR
                     VLDWKKARPRAFIAAVLTSVAAVSAASGYTVGAKPWAHSKARPALMAAKPAAKAKSTP
                     GVAARNLQARAPAAAPVVSRPAAAKPEALPTKGKGVLAMAPAGTHLQPAALKLSPQAN
                     MPRSNVATHLVANAKLNTAAKAPARVSNAAAPKSAGKAQPMAHAKAGLAAKPSVKPKV
                     AANAGHKTAVQGKGAAKPSAKSGKPALAQGKKPAHG"
     gene            complement(9071..10288)
                     /locus_tag="VFQ61_16650"
     CDS             complement(9071..10288)
                     /locus_tag="VFQ61_16650"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HET9956140.1"
                     /translation="MSPDAAPPSLRPESSPRTYASELDDTSAYAKLLKPLLFLLPAER
                     AHKVAFGALRLVLRLPFARRICRALLLPNDPTLRVRVLGLTLPSPVLLAAGFDKDAKG
                     FEALGALGFGAVEVGTLTAQAQPGNPGPRLFRLPADRALINRMGFNNEGAAAASDRLR
                     GPRSTVVGVNIGKTKVVSEDDAVADYVRSAELLGPHADYVVVNVSSPNTPGLRALQAV
                     DRLRPLLSGVRDALERVRPGRPPALLVKIAPDLSDEAVDEIAELALELGLSGIIATNT
                     TTSRDGLRTPEATVRGMGAGGLSGAPLRLRALQVLRRLRRKVGTRLVLVAAGGIESVD
                     DAWERIRAGASLVQIYTGFIYQGPLLPSRLAKGLASRAHAAGFASVQDAIGSSPERSS
                     LAPSPPQTAPVVS"
     gene            complement(10316..11329)
                     /gene="tal"
                     /locus_tag="VFQ61_16655"
     CDS             complement(10316..11329)
                     /gene="tal"
                     /locus_tag="VFQ61_16655"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET9956141.1"
                     /translation="MSSLLESLKKLTTVVADTGDIEAIARYRPQDATTNPSLLFKAAK
                     LPQYRSLVEEARAYSEKRGGAAEQRVNSFMDRLAVNFGCEILKLIPGRVSTEVDASLS
                     FDTEATIEKARTLIGLYESVGVSRERILIKIGSTWEGIRAAERLEKEGIHCNLTLLFS
                     FAQAVACAEAGVTLISPFVGRIYDWYKKASGTEPPVNDDPGVKSVVRIYQYYKKYDYK
                     TQVMGASFRRVEQITELAGCDLLTISPDLLEKLGATQGDLSAKLSLAAAKSLDIPRLT
                     ANQERLDEKRFRFLHNQDAMASDKLSEGIRLFYDDARKLESWAETALGSKAQGASAQS
                     AAH"
     gene            complement(11707..14250)
                     /locus_tag="VFQ61_16660"
     CDS             complement(11707..14250)
                     /locus_tag="VFQ61_16660"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET9956142.1"
                     /translation="MNPGKYKISAEELPLDPPALRESFVRHVQHTQGKHPIAATRLDH
                     FMAVARTARDRMFDRWTRTWYRRERQQPKMVYYLSLEYLLGRLLEDGLINLGIREQMR
                     EALEAVGVDLDQVVSQEPDPGLGNGGLGRLAACFLDSMATLGIAGIGYGIRYDYGIFR
                     QDLVGGTQTEAPDMWLQHGNPWEIARPERVYKVRFGGRVIQYTGSNGRLTHEWIDTHD
                     VLAMAYDVPVPGYHNNVVNTLRLWSAKATHDFDISYFNRGDYIKAVEQKFSTENISRV
                     LYPNDQQPAGKELRLKQEYFLVSASLQDSISRHLRYHPDLYNLHEQAVYQMNDTHPAL
                     AVAELMRVFIDDHGMPWDDAWALTRRCCAYTNHTILPEALERWPAWLMERVLPRHLQL
                     IYDINHWFLTDVRKRGATDEERLRRMSLIEEGPEKQVRMANLAVIGGSHVNGVSALHS
                     HLLRERLFRDFYELEPNKFINETNGVTPRRWLLKCNPELAKLITSRIGDGWITDLDQL
                     EQLVPLASDAEFQKEWQRVKRHNKERLANLLKRQFGIELEPTHLIDAQVKRIHEYKRQ
                     LLNILHVISLYLSYKQKPPHDAVPRTFIFAGKAAPGYDQAKRIIHLINAVAQVINHDI
                     AVRQFLRVIFVPNYSVTLAEYIIPATDVSEQISTAGMEASGTGNMKFAMNGALTIGTL
                     DGANIEIRDAVGEANMFLFGLNADEVIHAREGGYNPQQAYYAEPLLRAALDAVTSGMF
                     SPEEPGRFRPVVDPLLYGGDNYMVLADFLSYVHCHRSIDVAYHDPAGWTRRAILNVAR
                     MGRFSSDRTVLGYAKDVWGVYVPPAVKGLRLPPSLPPEPPSARTPNPAE"
     gene            14665..15354
                     /locus_tag="VFQ61_16665"
     CDS             14665..15354
                     /locus_tag="VFQ61_16665"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9956143.1"
                     /translation="MSDYARPKGAGLRRPTPAASRLIEHQSAPPIASTLWTQALGSVP
                     SELFLGQGSMGDARKLEVFECMLQALALTGTERVLEVGTGTGYRSALLAHMAKEVYTL
                     ETSPNAAELARERHARLGLNNVYVVDADGSKGFAIGAPYQAILVSGALPYVPTELIDQ
                     LDGGGRLVIPVGDDGGQLVERVRKLPQALETDTLTWCDLSSLICEAPPRASRRPWMRG
                     ASNRAAPSSGR"
     gene            15514..16389
                     /locus_tag="VFQ61_16670"
     CDS             15514..16389
                     /locus_tag="VFQ61_16670"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9956144.1"
                     /translation="MMQSSGRAGFIGGLGAQIALAVLASACGERPLEPRDPTPGVAYF
                     KIASFNIEVGDERDPETVGAIGATAADIICLQEVSAEWEPVIRARYSADYPHMLFHPG
                     GAGGLGVLSKWELAEGEFIDASEEAHPAWSLFASTPAGTMNILNVHLRSLYSGSGGVV
                     NDYLTWGSDHVVELRSVVASEPTRVVESLPRIVVGDFNEGTNGDAVAYLERLGFSDAL
                     PLYHPGQFTWRHASLGNQFTESLDHVLFDEWFAPLNAFVLVKGNSDHIPVVAHLEAAR
                     AWPSVTLSQLSTASE"
     gene            16439..18727
                     /locus_tag="VFQ61_16675"
     CDS             16439..18727
                     /locus_tag="VFQ61_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956145.1"
                     /translation="MRSPVNYAVLAFALATSISGLSRAETLQAPIGGKWISLGPARVA
                     CEQPGGWSVDPSGHQLRPPAQAEAIGKSVELKIAADASGCAASSNTIRLVATGPWPQL
                     DSNTVVLLPDQNAIEARGRRLQGVGVAWRTESAAGLDVCQPKLEANAERCVWTIGRGV
                     SADPSAGQLSWVPLGGRAGPDVSTFDSDGVRADAATFALVPQQVSLTALLPPDAVIDL
                     SSGRGQVPLLHPEVIRSVECAPARCELGGGKLWVRGLGSDVSAVEVRFGLAPKVGLAK
                     KEGLDSKPHAHLNVLHCPMTVPSGPPLRDVDGARLVVRLDEHCARNLNALTFEVDDRP
                     VRVLQSHSDARETFVLLDVGRLDRDAIAIRARPKQGDDDDVVALTRVDTMKPPRNRSS
                     LEIPGHKNLDFIPNNRPARVHVAPVGAPGRLAVVPVEGVYHVPSDAPDQIVGDPDAAG
                     LVTLRFAYRVETLPEEFRNVDLAVMDDVLQRSVHEANLPVPLGGSVFGPNPLVELVCG
                     PTLHGKRVQPGETERLPFTSRDDCRLVFHRERMPRDYGSQKLTLNIEVVNPDGAVRPD
                     GRVAQTLTLRPGRVPVYAWIHGVQNEFDRVIVRLSHAADEAHYIGASEIDTGSPAVKW
                     TVVFGRGRARLYATTAIPTGLYRFGDPAHSGLLSLNFGVVSRLTWLNEDGREGFLGLE
                     TGIMAIGLGNDRSANGESLTQIGAVGGLGVAVPIANRSAPTQASINLHGWVEVDISHT
                     EGNRFAFILGPSVSIGNVGANL"
     gene            complement(18756..20285)
                     /locus_tag="VFQ61_16680"
     CDS             complement(18756..20285)
                     /locus_tag="VFQ61_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956146.1"
                     /translation="MAWNVDEGPVYGLTSQTWQWVAWLAARAFRDYFVLTMRVISAAL
                     LLATHAVLLYGTRERDSGLAATYAICSPVCLFMALSGMETPLALFITSVILCLVYGAN
                     DSPGVNPTRVRRWSFAIAPLLAVLLYVTRPDALALVLPPLLVERYARTKVLPWRELLI
                     VLLGVGTFLALFKLVYGTVLPLPFYAKTAAFSPYDDHFRALSREVQRIRFGTLAFVAL
                     PLVLGALLRRDRMNWTLLATAALFELYHLAGTIDVMGMQGRFYAPALPLLVFAGARGL
                     AGVKAESEAKASWLVPRGYLRCVRLVSYAAGVALLLRFHFVPTADDWFMDQVPPSLIV
                     SCILFGSVLSWPVVTAVRRPLVPAALALGTAIASWNLGKPELLNDDAYLTRHASNVTT
                     YRGLDALVKCLGDSLHIYHSEVGVVGLRFQNGKVTDLAGLLSPRWLFRGSKSFDQLCE
                     EDDPEAIFLPHKNYVTLNREIRGGQCLRNYARVVSRSSSPLYVRKDLLLQYRRCAPGK
                     P"
     gene            complement(20246..>20536)
                     /locus_tag="VFQ61_16685"
     CDS             complement(20246..>20536)
                     /locus_tag="VFQ61_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956147.1"
                     /translation="PLRVARTRRELEFSAGYSEVALDVARRGGLVCEQMRRFERPMVW
                     LRVCVAVALAVFVWGMLGREPYDDSYFFKRVAVNFIRPGAWPGTSTRAPYTD"
BASE COUNT         3649 a         6717 c         6492 g         3678 t
ORIGIN      
        1 ggccgtaccg gggctgcgat tgggctattg tgtgttgccc ccgagctggg cggagcggct
       61 cgaagcagag cggccgccct ggtcggtgaa tgcggcggcg caagcggccg tgcgagtcgc
      121 ttgcgatccg agcgtcacga gcttcgtgca gcgcagcaaa gaacgactgc ggtcggatcg
      181 aatcttcctg cacgagcagc tcgaacagct cgggcttcga gttcacacca gcgtggctcc
      241 ttactgtctc gcaaagctgc cggagcccct gaccgcgacc gtggtacgca gtgagctcct
      301 cgagcagcac cgggtgctgg tgcgagacgc gacctcgttc tgcttgccgg gacatgtgcg
      361 tgtcgccgca cgaccgcctg agcaagcact ccgactcgtc tcggcactcc gcagtacact
      421 cgggcgcagt acactcgggc gtacgcgaca cggaagctaa cctgcttgcg gtccgctcga
      481 gtcctgagcc agccagctcc ccgacgaatt ccaaaaattc gaggcagctg actcggccgg
      541 ctgtattaga ctcgccgcgc tatgcggaaa ggtgtgttct cgtcaggtgc gccgagcctc
      601 gggctcggta tgtttgccct cgtctcaggg ctcttttgta cggcatgccc ggagaagaag
      661 gaaccggctg ctgaccccga gccaaaagca gccgcggccg agccaaaagc agccgcgccc
      721 accccgggtg ccgcagacaa ggatgatgac gacgcgaaac cgccagaacg ggaagaaaag
      781 aaggaccaag gcggctggta acggggttgg tgcgggcgga ctaggggccc ccagaacaca
      841 atttcgggca gacctttatg gcgagaaagc gctacgtcgt gatcggcgaa ggtgcggccg
      901 gtctcaccgc agtggaagaa ctgcgtcgcc tggacgccca cgcggtgatc ggcttgttca
      961 gcgacgagcc ttaccccgcc tacttccgag ccgctctgac caactacttg ctcggtgaac
     1021 tccgcgaaga tcagctgttc gtccaagcgc cggatttctt tcaagccgcg cggattcgaa
     1081 gagcgctgac gcgcgtcgtc gccatcgacc ccaagcgtgg cctggtttgg gacgcgggga
     1141 gtgcgcaacc cactccgttc gacgcgttgc tcgtggcgag cggcgcttat gcacgcccgc
     1201 ccacgttcga gggcggggcc ctgcccggtg tcgtcactct gcgaactcta tatgacgcgc
     1261 gggtggtgat ggagtggctg cggctcggca gcctcaagag cgccgtgatc ctcggcgcgg
     1321 gcccgctcgg cttggagtgg gcccacgctc tgctcgagca tcgcattcag gtcaccatca
     1381 tcgaacgagg caccagactg ctggcttcgg cgctcgacga ggtcgcttcc gatctgttgg
     1441 cggcgcgcct ccggcaggcg ggcgtgcagg tcatctttgc cgatgaagtc gtgcgcgctt
     1501 acgcgggccc ctctgggagc gtggctgggg tcgtgttgag aagtgggcag caactcgcct
     1561 gtgggctcgt tgcggccgcg ttgggcgtgg tccccaacac gggcttcctt tccagctcgg
     1621 ggttgaagct cacccactcc ggcgctctgg ccgtagacgc cacattgcgg agctccgaac
     1681 ccaacgtctg gggtgctggc gacgtggtca gcgtcaacgg ccagtccctc gcgctgtggg
     1741 aacccgccaa gctccagggg cgcctcgccg cccgcaacat ggtcggcggg cgcatgcaat
     1801 acgagccagg cacgcactac tttgccactc gcttattcga tctggatttc gcgcggctcg
     1861 gcagtatcga tgcggcaccc acccgagatg tgctggtgga tcttcctcgt ggcacgggca
     1921 agattgctta tcggaaactg gtattcgagc agggaaagct gatcggagcg ctcatgatgg
     1981 gagagcgctc ggagcgcgtg cgggcgctgg ggcgaacgct caagcgcctc atcgatcaac
     2041 gcatcgatgt ctcgtcgatt cgcgacgccc tgctcacgcc cgagttcgat ttgagcggct
     2101 ggctcgaccg gcagaagcgg gttgcccaac ccacacccgg acagaagacg cagtccgaga
     2161 tcccgctcga ggccaagctg cgcggtacgc aaggactcgc gctgagccaa ctcactcaac
     2221 acgtggctgg gctcactcca ggcgctcaca gcggaggcaa cgcgacgggc gccatggcca
     2281 tgcccacgcc ttccggtggg gctccttttt cggtattcgc ctccccctcg aacgcatcca
     2341 gcggcgcacc ggtcggtcag ggcccagccc gcgaagcgcc gcgggccact cgcatgctct
     2401 cgattggatt gcccgccgag ctggcccagg cgcagcccaa agatgtgccc agccgaaagg
     2461 cttatctcga ataccgaggc agtcggcggc ccatcgagcg gcgcgtcgtc cgattgggcg
     2521 ctgatgcaca ctgcgagctc ctgctcgatg cggccggcgt cgagggcatg cacgcagagc
     2581 tctgcgaata cgcgggctct ttctatgttc aggatcttgg aacccgcgcg ggcacgtggc
     2641 tcaacgcgcg ccccgtcacg agcgccgagc gactgcgcac gggtgatcgg ctccgaatcg
     2701 gcgccgccga gctggtcttt caggtcgagt ccgaacccaa agaagaacga tccgatggcg
     2761 ccactccgcc ggtgccacga ggtgacttgg tcgccgcccc ccgactgctg gttcagtccg
     2821 gggcgaagat cgggcacctc gtgcggctcg cgcagcgccc gtgtttgatt ggtcggagcc
     2881 cggcgtgtga cctcagtctg aacgagcctt tcgttgcgcc cgaacacgcg cgcgtgttcg
     2941 cgcaaggttc tgagtttttt ctcgagcccg tcgagaacgc ctcggcgctg gacccacgct
     3001 tcggaacttg gctccaagac agccctctgt atccggggca gcgtcagctg ctcacggaaa
     3061 actcgagaat tcggatcggc agcgtcgagc ttgccttcac gcgtgcggcg ctgccggacg
     3121 ccgccggcgc gctgatgccg ggtggccgcg tggtggtcga ctcaggtcca gacttgggaa
     3181 aaacccagtc ggtcgggcca gggctgcgga ttgggagcgg accggaagcc aacttcgtgc
     3241 tgcaagggct cccggcattg gtactcgaga tcgcgcctca cgaacgcgag ttcttcgcgc
     3301 gggacttgag ccgtgggcac gcactgcggg cgggtcgcag cctcggagac gcgtgggttg
     3361 cgctcagcca cggtgaacaa ctcctgctcg ggggctcggt cttgctgcgt ttcgaggaag
     3421 tatgaccatc ggggctcccg cggcacagga gctcagcccc tatgaaacag agctcgctcg
     3481 acgtgtcgag gctcaagccg ccgcttgcat cggttgcaac gattgcctgt tggcttgccc
     3541 gatttccgag tcggcgaacg tcaccatcgg agagttgaac cgggctttgt tcttgcccgt
     3601 cattcaagag cgccgcgtca tcgaattcgt aagcgcctgc acgcagtgca accagtgcgt
     3661 ccccgcctgc cccgcggatc tgagccgggc cgagatggtg ctgttcaaca agctcaaagt
     3721 ggaagacacc gtggctgact acgagctctc gctcagcgct cgcacggtga cctttccttc
     3781 aggcatgcgc ctcgatgagc tcgctcgcga gctcggcaaa ctcgagctgt ttcgaggcgc
     3841 ttcgtttttg gccctacggc gtctgattca gaagagcacc ttgcgcttgc tggtaccggg
     3901 cgagacgctg tgttcggaag gcgagtacca cgagcggcta agcgtcgtgc tgaaaggcgg
     3961 cctgggccaa tactcccgag gtgcgcgcgg agagtccacg cacttgctca cgctcgggcc
     4021 agggagcttc ctcggcgagt tgagcgtgct cggcggagcg ccggagccgc acacgataat
     4081 cgcccaaacc accagcgtgg tcctcgacgc acccaaagcg gcggtgctgc gcgtgatggc
     4141 tcaagcccca gacgtccgcc atgcactcga gagtctgtac gccaaacgcg ccctgtatac
     4201 ctatgccaag agccccgggg cgctgggcgc gctgccggac gaagcgattc gctatttgct
     4261 cgagaacgcc agcctggagt tcatggcctc cgggcagcgc ttgttcgaga tcggggagcc
     4321 gccgcaggct ttgttcctcg tgcgttcggg gttcttgcgc gccgtacagc gtgatgccca
     4381 gggcgagcgc gtgctcacgt atttcagaga aggtgacgtg ttcgggttgt tggggctgtt
     4441 ggagcgtgac gcgggccagc gctatgcagc gcacgctgcc agtcgagcgg aggtcatccg
     4501 tattccttcc gcggtgctct tcgccgtgct cgctcaccac ccgaccgccc aggcagcact
     4561 cgcgcgtgcc gcgtatcaag ccgagcagtt ggcccgctcg ggggagattg ggctgggtcc
     4621 ggtgccgcag gccgcccgcg cgcgctcgct ctcgggttca cctgagccga gcttgcccgg
     4681 agagcgggag cggctcggag cgtcggtcct cgtggagcgt gggttagcga gcggccgcga
     4741 ggtgctcgtg attgaccaga cccgctgcac cgcctgtggc aactgcatcg actcgtgcga
     4801 acgtcgccac ggagcgagcc gcctgagcct gcgcggcatt caagtcgacc agtacctgtt
     4861 cccgagtgcg tgccgacact gcgcggatcc ggcctgctta ctctgcagcg tcaacgggat
     4921 cgtgcgcctg ccgagcggcg agattcaaat cgttcgcgac acatgcatcg ggtgcggagc
     4981 gtgcgccgag cgctgcccct acggcacgat cagtatgcac gcggcccaaa cgccgaagcc
     5041 cagcctctgg gcgtcgttgt tggatttctt ggccggctcc acgcgagagg aatccgtgcg
     5101 cgtggcttct ccggacgagc cccgcgtcgc cgtgaaatgt gacttgtgcg cggggcacgc
     5161 ggactacgcc tgtgtctcgg cttgcccgac cgcggccgct ttccgcgtca accccgatcg
     5221 ggtgctcggc gagggtgaag gatcgaaccg ctcggcatca tcaccgcgcc gaggcaaacc
     5281 ttgactggtc ctctgctcca gcggcgcaaa acgtggctcg aacgcgcctg gatctggctt
     5341 cttttcggca gcgctgcggc gggcgtgagc ggcgcggctg cattgttcat ctattcggga
     5401 gggctctcgg atttcgaaat cttccccgcc gtcgcccgcg gctacacgcc gctaggcttg
     5461 acctacggcg tactcgccac ggtgtgcctc gcggtcactt tcttctattc atttcggcgg
     5521 cggcctttgc aagaggcgct tcctttaggc aagggcagca tggccgcgtg gctttgggtg
     5581 cacgtggtgt tcgggactct cgcgctctgg ttcgcgttgc ttcacgccgg cgctggcaac
     5641 ctcgcgttgc gcgcttcatc tggcaaagcc ctgctgattc tgctcgcgct cattgcgcta
     5701 ggtggcgcgc tctggcgttt gctctatctg ctgctgccca agcgggcgac ccgcgaggtc
     5761 ggcaactatg ccacggagga aagtcgggct cgcagtgacg cgatagccat cgagctggag
     5821 aagctcgccg ccggccgctc cccgacgctg caacgcctga cctcgcaact gttcgaggcc
     5881 cccctcacgc tgtccgaggt cgcgtccttg ggcgccaccc tcccgcccca agaacgggca
     5941 gtgttcacgg agatcgcgcg gctcaccgcc gagcgacacg cgagcttggg gcgtgaagcc
     6001 aagcaacgcc gctacgtcgc acgactgcaa ggtatgcggc tctatcacgt ccccatcagt
     6061 ctgctgctcg cgatcgggtt gccactccac gttgtattgg cgttcgactt gcccgagcgt
     6121 ctgctggcaa acacgcgcgc cgcaccattg gttgcgagct tcgagcccgc gcagacctgc
     6181 gctcagtgtc acgctcgcgt ggtcgaagag tggcgcagct cgatgcacgc tcatgcgctc
     6241 agcgggccga tcatgattgc gcaaaccaac ctggcggcgc gcacgacctt gcaggcgggc
     6301 ccgacgcccg accccaagca gatatgcgtc aattgccatg gtcctttggc cgcgcgctta
     6361 tcgcccagcg ataagctgcc ggtgcccgcc ctgcagacca cgacgctgac tccgtggctc
     6421 gacgcacaga acagtctgct gactgatggc gtgaattgca tcgcctgcca cgccgcggac
     6481 ggcgagcccc atacgggagg agccgcgctg accgcgttcc aatccacgct cgcagcgggg
     6541 cgaacctacg gcggcgcgct cgatcaaccg gtgggcaacg cgttccaccg cagcgtagac
     6601 tcgagtcacg cgagcgcccc tgagcggctc tgccaaagtt gccattcggt cgtgtatgac
     6661 cgcaatggag acggcaagat cgagaagggc ccggatctgg tgcttcaaga tctgttctcg
     6721 gagtggcagg cctatcgggc ctcgggcggc gcgcactgcg tcgactgcca catgcccctg
     6781 ctcgggaagg gtcgcgcggc cgagtccgct cggattccca tcgagcaaga cgacgaagca
     6841 ccgccccgcc ctttacgcag ccatcgcttc attggcccgg actacccgct ggatgagccc
     6901 gccctacggg atgcgcatcg ccccgcccgc accgcgctgt tgcagcaggc ggccaagctg
     6961 agcgtggttg ccggctccgt ctcgttcgtc gacgggcggg cgcaggtgcg gctagcggtc
     7021 gcgaataccg gcgtgggtca caacctgccg ggcggcttcg cgttcgttcg gcaaatgtgg
     7081 atcgaactcc aggtgctaga cagcgccggc cgagttgcgg cgcagacggg cacactccgc
     7141 cgctcgagcg acgacttgtg tgacgcggag ctgttgcgtg atgcgaaccc actgcgcggc
     7201 tttgccgtcg gttgcctcaa gccagacgaa tcgctggtca acttccagca gctgcttttg
     7261 gatcgtatcg agttcacgcg ggacggcggc ggacgcgccg tggtgacgcg cccaccgggc
     7321 gcggtcgaag tggtcgttca acacctgact tcaggcagcg tgcctcgcgt gcggccgttc
     7381 gatcgtcgcc cgactccacc actgagcccg ggagaatcac gaaccttcga cttttcgctc
     7441 gagctgccga gcggaaccac gcccgcccag cttcgcgcgc gactcttgtt ccgggctgtg
     7501 ccgccctatt tcctcagggc actcgaggct caacaacagc ccgaggatgg gcctccgttg
     7561 agccgttgga tcgatcagat cgagatcgtc gaaatggcga gcgtcaccgc tgccttcaac
     7621 cgttgaaaag cccagcccgg ttcagccgtg tgccggcttc ttgccttggg ccagagcggg
     7681 cttgccggat ttggcggacg gcttggcggc tcctttgccc tgaactgcgg tcttgtgccc
     7741 ggcgtttgcg gcaaccttcg gcttcaccga tggctttgca gcgagacccg ccttggcgtg
     7801 tgccattggc tgagccttgc ctgctgattt cggagccgcc gcgttgctca cccgcgccgg
     7861 agccttcgcc gccgtattca gtttggcgtt agccaccaga tgcgtggcga cattggagcg
     7921 cggcatgttc gcttgcggac tcaacttgag cgccgccggt tggaggtgcg tgcccgctgg
     7981 agccatcgcc agtacaccct tgcccttggt gggaagagct tccggttttg cagccgcggg
     8041 acggctgacc accggagcag cggcaggagc tcgcgcctgc agattgcgcg ccgctacacc
     8101 tggagttgac ttcgctttgg cggcgggctt ggcggccatt agagcgggac gcgctttgga
     8161 gtgcgcccaa ggcttggccc caaccgtata tcccgacgca gcggaaactg cggccacgga
     8221 cgtaagcacg gcggcaatga aggctcgggg acgcgccttc ttccaatcca ggactcgctg
     8281 cttcatgcgc gccaaacgcc catcgactcg agtaaactcg gcctcgctgg agccgtcgcc
     8341 cgctgcgccc ttcggaacga gcggaattac caactccggc tcctcatagg tataaggcac
     8401 gccgtcctcc gcaaaggagg gcatgggtgc ttcgggcgcc acttgcgctg cgggccactc
     8461 cgagctcggg cctgcggcgt tccgccgacg gagcgggggc ggagcgggcg ggggcggggg
     8521 cgtcggtaac accgacgaag gggcaggatc ggccggataa cgctgcgaag gatgcgacat
     8581 ggaaaatcct cccgaacgac ggagcgcgcg gatgcacccc gcagtgaagc cgcgcacccg
     8641 agtgaggggc gcgagctgct gggcaatctg ctaatcagct gggcgggcct gctaggaaat
     8701 ctgctgggca ggtctgctgg gaatgagttc actcgcgaac cggactacgc gaggatgaac
     8761 gtacggagtc tagcttacgg gatacccgtc gcgcacggac cgtgtgacga aatatcgagt
     8821 ttagatttca ccccgcccgg gattttatct cggttggcaa aaagcctgtc agtttcggtt
     8881 cactcgaggc gcgtcaaact cacgatcttc gtagcggggt aaaccgacgt ggcgcaccac
     8941 cccgtgggag tgaatcgacg ctcaacactt cgacctacat gagcaccgga gctcgatgtt
     9001 ggaatcgagg aacggacccc aacgctctgg tcgatcgaac cccgaacacc gctcgctaag
     9061 acacggctcg ttaggagact accggtgcgg tctgcggcgg gctcggtgcc agtgatgaac
     9121 gctcggggct gctgccaatc gcgtcttgca cggacgcgaa gcctgccgca tgcgcccgtg
     9181 acgccagccc cttcgcgaga cggctcggga gcagcggccc ttggtagatg aaacccgtgt
     9241 agatctgcac caggctcgcg cccgcgcgga tgcgctccca ggcgtcgtcc acgctctcga
     9301 tgccgcccgc agccacgagc acgagacgtg tacctacctt ccggcgcaac cgacgcaaga
     9361 cctgcaacgc ccgcaagcgg agaggtgccc cggatagccc gcccgcgccc atgccgcgca
     9421 ctgtcgcttc gggcgttctc agaccgtcac gactcgtggt ggtgttggtg gcaatgatgc
     9481 cggagagacc gagttcgagc gcgagctccg caatctcatc gaccgcctcg tccgaaagat
     9541 cgggcgcaat cttgacgagc aacgccggcg ggcgcccagg acgcactcgc tcgagcgcat
     9601 cccgcacgcc ggacaacaac ggacgcagac gatcgacggc ctggagggca cgcaaacccg
     9661 gcgtgttggg cgagctcacg ttcacgacca cgtagtcggc gtgcggcccg agtagctccg
     9721 cgctccgcac gtagtccgcc accgcatcgt cttccgaaac gaccttcgtc ttcccgatgt
     9781 tcaccccaac gacggtcgag cgcggcccgc gcaagcggtc cgaagcagcc gcggccccct
     9841 cgttgttgaa acccatgcgg ttgatgagcg cgcgatccgc cggcaaacgg aacaggcgcg
     9901 gcccaggatt tccaggctgc gcctgcgcgg tcagcgtgcc cacctccact gccccgaagc
     9961 cgagtgcccc gagcgcctcg aaccccttcg cgtccttgtc gaagcccgcc gccaacagca
    10021 cggggcttgg caacgtcagc ccgagcacgc gcacgcgcag cgtcggatcg ttcggcagca
    10081 acaacgcccg gcaaatgcgc cgtgcgaacg gcaggcgcag caccagccgc agcgcgccga
    10141 aagcaacctt gtgggcccgc tccgcgggga gcaggaacaa cagcggcttc agcagcttcg
    10201 cgtatgcgga cgtatcgtcg agctcactcg cgtacgtgcg agggctcgac tcgggacgaa
    10261 gactcggagg ggcggcgtcg gggctcatgt gccgtgaagt ggcggttcga gatgctcaat
    10321 gcgcggcgct ttgagcgctc gcgccttgtg ccttactgcc gagcgcagtc tccgcccagc
    10381 tctcgagctt gcgtgcgtcg tcgtagaaca ggcgaatgcc ttcgctcagt ttgtcactcg
    10441 ccatggcatc ctgattgtgg aggaaccgga agcgcttttc gtccaggcgc tcttggttcg
    10501 cagtgagccg cggaatgtcg agcgacttgg ccgccgcaag cgacaacttc gcgctcaagt
    10561 ccccttgagt cgcaccgagc ttctccagca ggtccgggct gatagtgagt agatcacagc
    10621 cggcaagctc ggtgatctgc tcgacacgcc ggaagctcgc gcccatgacc tgggtcttgt
    10681 agtcgtactt cttgtagtac tggtagatgc gcacgacgct cttcacgccg gggtcgtcgt
    10741 tgaccggcgg ctccgtgccg ctcgccttct tgtaccaatc gtaaatgcgc cccacgaacg
    10801 gcgagatcag cgtcacgccc gcttcggcgc aagccacggc ctgagcaaag ctgaagagca
    10861 gcgtcaggtt gcagtgaatt ccctcttttt cgaggcgctc ggcggcacgg atgccctccc
    10921 aagtactgcc gatcttgatc aggattcgtt cgcgcgaaac accgacgctc tcgtacaggc
    10981 caatcaacgt gcgagccttc tcgatcgtgg cctcggtgtc gaagctcaga ctcgcgtcga
    11041 cctcggtcga gacccgcccc gggatgagct tcaagatctc gcaaccgaag ttcacggcca
    11101 agcggtccat gaacgagttc acgcgctgct cggcagcgcc gccccgcttc tcggagtacg
    11161 cgcgcgcctc ctccaccaac gagcggtact gcggcaactt ggccgccttg aacaggagtg
    11221 aaggattcgt cgtggcgtcc tgcggccgat aacgggcaat ggcttcgata tcgcccgtat
    11281 cggcgacgac cgtagtgagc ttcttcaacg actcgagcag ggatgacatt aaaggcctcc
    11341 gaatcagcgg agctggggtt ccggcgactg ccggggaaag agaggtttcc tgaatatgtt
    11401 cgggtcagga tgctcccatc ccacactgtc caacgcaagt ctgtttaaga gcgcggcgag
    11461 cgtggacgcc gcccttccga cgtgcagcgc aactgttctc gtccgggtct cgtcctggga
    11521 tgagagcgac ccggccccaa tcgttccgct ctgcgtcagc ggcttctcac acctgcgcac
    11581 ggacgtttgc cgcgcatttg tgcccatttc taactgtggc caatcgggag aggttcgagc
    11641 cgcgcgccga gcgcggctcg ataccacgta gacctctcac cctggacaac agctcgcgtt
    11701 ttcgcgtcac tccgccggat tgggcgtacg agcgctcggc ggctccgggg gcaagctcgg
    11761 tggcaaacgc aaacctttga cggcgggtgg cacgtacacg ccccagacgt cctttgcata
    11821 gccgagcacc gttcgatcgc tggagaagcg acccattcgt gccacgttca ggatcgcgcg
    11881 ccgcgtccaa cccgccggat cgtgatacgc gacgtcgatc gagcgatggc agtgcacgta
    11941 gctcaggaag tcggcgagca ccatgtagtt gtcgcctccg tagagcaacg gatcgacgac
    12001 cggacggaag cgccccggct cttcgggaga aaacatgccg ctcgtgaccg cgtcgagcgc
    12061 cgcgcgcagc aatggctcgg cataataagc ctgctgcggg ttgtaaccgc cctcgcgcgc
    12121 gtggatcacc tcatccgcgt tgagtccgaa gaggaacatg ttggcctccc cgaccgcgtc
    12181 ccggatctcg atgttggcgc cgtccaacgt gccaatcgtc aacgctccgt tcatggcaaa
    12241 cttcatattg ccagtgcccg aagcctccat gcccgccgtc gaaatctgct ccgacacatc
    12301 ggtcgccgga atgatgtact cggccaacgt cacgctgtaa ttcggcacga agatcacgcg
    12361 caggaactgg cgcaccgcga tatcgtgatt gatgacctgc gccaccgcgt tgatcaggtg
    12421 gatgatgcgc ttggcttggt cataacccgg cgcggcctta cccgcgaaaa tgaacgtccg
    12481 aggcaccgcg tcgtgcgggg gcttttgctt gtagctcagg tacagcgaga tgacgtgcag
    12541 gatgttgagg agctggcgct tgtactcgtg aatacgcttc acctgcgcgt cgatcagatg
    12601 cgtcggctcg agttcgatac cgaactgccg cttgagcaaa ttcgcgagac gctccttgtt
    12661 gtgacgcttg acgcgctgcc attccttctg gaactccgca tcagaagcca acggcaccag
    12721 ctgctctagc tgatccagat cggtaatcca gccgtccccg atgcgcgagg taatcagctt
    12781 cgcgagctct ggattgcact tcaacagcca gcggcgcggc gtcacgccat tggtctcgtt
    12841 gatgaacttg tttggctcga gctcgtagaa atcgcggaac aaccgctccc gcagcaagtg
    12901 cgagtgcagc gccgaaaccc cgttcacgtg gctcccgccg atcaccgcga ggttggccat
    12961 gcgcacctgc ttttccggcc cctcctcgat cagcgacata cggcgcagcc gctcctcgtc
    13021 cgtggcgccg cgtttacgca cgtcggtcaa gaaccaatga ttgatatcgt aaatgagctg
    13081 caggtgccgc ggtagcacgc gctccatcaa ccacgccggc caacgttcga gcgcctccgg
    13141 caggatggtg tgattcgtgt aagcgcagca gcggcgggtc agcgcccagg cgtcatccca
    13201 tggcatcccg tgatcatcga tgaacacccg catcagctcg gccactgcga gcgcggggtg
    13261 cgtatcgttc atctggtaca cggcctgctc gtgcaggttg tacaggtccg ggtgatagcg
    13321 caggtgccga gaaatcgaat cctggagcga ggccgagacc aagaagtact cttgcttcag
    13381 acgcagctct ttacccgcag gctgctggtc attcgggtac agcacccggg agatgttctc
    13441 ggtcgagaac ttctgctcga ccgctttgat gtagtcgccg cgattgaagt acgaaatatc
    13501 gaaatcgtgc gttgctttgg ccgaccacag ccgcagcgta ttgacgacgt tgttgtgata
    13561 gcccggaacg ggcacgtcgt aggccatcgc gagcacgtcg tgggtgtcga tccactcgtg
    13621 cgtgagccgc ccattgctcc cggtatattg aatcacgcgg ccgccgaaac ggaccttgta
    13681 gacacgttcc ggccgcgcga tctcccacgg attgccgtgc tgcagccaca tgtccggcgc
    13741 ctcagtctgc gtgccgccga ccaaatcctg acggaagata ccgtagtcgt agcggatgcc
    13801 atagccgatc ccggcgatac ccagcgtcgc catggaatcg aggaagcaag ccgccaagcg
    13861 tcccaaacca ccgttgccga gcccgggatc cggctcctgg gacaccacct gatcgagatc
    13921 gacaccgacg gcctcgaggg cttcccgcat ctgctcgcgg atgcccaagt tgatcaaccc
    13981 gtcctctagc agccgcccca gcaggtactc gagcgagagg taataaacca tcttcggctg
    14041 ctggcgctcg cgccgatacc aagtgcgcgt ccagcgatcg aacatgcgat cacgagccgt
    14101 gcgggccacg gccatgaaat ggtccagtcg cgtggccgcg atcggatgct taccctgtgt
    14161 gtgctgcacg tgtcgcacga agctctcgcg caacgcaggg ggatcgagcg gcaattcctc
    14221 ggcggagatc ttgtattttc cggggttcac ggtgtgcttg aataccaatc ctgtccgccg
    14281 tttaccagct tccttctcga agtgccgtgt gttcgatcgg cttctttcca cgacgcaacc
    14341 gccacgtttc gcgcgtgaga ttcgatccct gcgcggcccc cgagcctgaa ggcacgcccg
    14401 agcccgaagc cgacctcagg ctcgtcgtga ctcaatgttc atgacgccgc tccgggggat
    14461 ccggtgggcc attgccgatg agttgatccg gacctcgaaa gcaggtaggc cataatcgcc
    14521 cgttgtaggc ctccttcgtg caaaacgcgc agtctttgcg ggtccgacgc tgaggtttgc
    14581 gcataaccgc tcagcccgat agcagaaact gcaaggacag tgggcctacc cgcgaggcac
    14641 actccttgct ccacctggct tgctatgtcg gactacgctc gcccaaaggg tgcggggctg
    14701 aggcgtccaa cgcccgccgc cagccgcctg atcgagcacc agagtgcgcc tccgattgcg
    14761 agcacgctct ggactcaggc cttgggctcg gtgccatcag agctgttcct tggccagggt
    14821 tcgatgggtg atgcccgtaa actcgaggtc ttcgagtgca tgctgcaggc attggcgttg
    14881 accggaaccg agcgtgtctt ggaagttggc acgggcactg gatatcgctc cgcgctgctc
    14941 gcgcacatgg ccaaggaagt ctacaccttg gagaccagcc ccaacgcagc cgaactggcg
    15001 cgtgaacggc acgctcgttt agggctgaac aatgtctacg tagtcgatgc ggacggcagc
    15061 aaaggatttg cgatcggcgc gccctatcaa gcgatcttgg tgagtggggc cctgccctat
    15121 gtcccaactg agctaatcga ccaactcgac ggcggagggc gcctggtgat tccggtcgga
    15181 gatgacggcg gtcagctcgt ggagcgagtc cgcaaactac cgcaggccct ggaaaccgac
    15241 acgctgacgt ggtgcgacct ctcctctttg atttgtgagg ccccgccccg cgcgagccgc
    15301 cgaccctgga tgcgcggcgc gtcgaatcga gctgcgccga gctctggacg ttgatcagct
    15361 cgatcgcgaa ttgacataaa gcgcggccgc ggcttgccgt tcgcgtgtga ctgcatcacc
    15421 ttgtttcgag cagctggatc aaacgcagct ggattacgag gcaatcagct cacacgccgc
    15481 agtgtccatg acgctgccga ttgacggata ccgatgatgc agagttctgg tcgggcgggt
    15541 ttcattggag ggctgggagc ccagattgcc ctcgcggttc tggcaagcgc ctgtggagaa
    15601 cgaccgctcg agccgcgcga tccgacgccc ggcgtcgcct acttcaagat cgcgagtttc
    15661 aatatcgagg ttggagacga acgcgatccc gagaccgtag gcgcgattgg cgcgaccgct
    15721 gcggacatca tctgtctaca agaggtatcc gcggagtggg aaccggtgat tcgggcgcgc
    15781 tactcggcag attatccgca tatgctcttc cacccgggag gtgccggagg cttgggcgtg
    15841 ctctcgaagt gggagctcgc cgagggcgaa ttcatcgatg cgtccgagga ggcgcacccc
    15901 gcctggtccc tgttcgcgtc gacgccagcc ggaaccatga atatcctcaa cgtacacttg
    15961 cggtcgttgt actcgggcag cggcggcgtg gtgaacgact acctgacttg gggctccgat
    16021 cacgtcgtcg agctgcgcag cgtggtcgcg agcgagccga cgcgcgtggt cgagagcttg
    16081 ccccgcatcg tggtgggcga cttcaacgag ggcacgaacg gggacgcggt cgcgtaccta
    16141 gagcggctcg gcttctccga cgcattgccc ttgtaccacc cagggcagtt tacttggcgg
    16201 cacgcttcgc tcggtaatca gttcaccgag tcgctcgatc acgtgctgtt cgacgagtgg
    16261 ttcgcgccgc tgaatgcatt cgtgctagtg aaaggcaact ccgatcacat tccggtggtt
    16321 gcgcacctcg aggcagcgcg cgcttggccg tcggtgaccc tgagccagtt gtcgaccgcg
    16381 agcgagtgag atcgcccgca gcttgcgctc caatcccgct tgagccacgc tgcgcgccgt
    16441 gcgttcgccg gtcaattatg cggttttggc tttcgctctg gctacttcga tcagcgggct
    16501 ctcgcgtgcc gaaacgctac aagccccgat tggtggcaag tggatctcgc tcgggccggc
    16561 ccgcgtagcc tgcgaacagc ccggcgggtg gagcgttgat ccgagcggac atcaattgcg
    16621 cccacccgcg caagcggaag ccatcggaaa atcagtggag ctcaagatcg cagccgacgc
    16681 gtcgggctgc gccgcctcca gcaatacaat taggttggtg gccacgggac cttggcccca
    16741 gctcgactcc aataccgtgg ttttgctccc ggatcagaac gcgatcgaag ctcgcgggcg
    16801 tcggctccaa ggcgtgggcg tggcctggcg cacggagagc gccgcgggac tcgacgtctg
    16861 ccaacccaag ctcgaagcca acgccgagcg ctgcgtgtgg accatcggtc gcggcgtctc
    16921 tgccgatccg agcgccgggc aactctcatg ggttccgctg ggaggccgcg ccgggcccga
    16981 cgtcagcacc ttcgacagtg acggggtccg cgccgatgcc gccacgttcg cgctggttcc
    17041 acagcaggtc tcgctcaccg cgttgttacc gcccgatgcg gtgatcgacc tatcctccgg
    17101 gcgcggccag gttccgctgc tccatcccga ggtcattcgc agcgtcgagt gtgcgccggc
    17161 gcgctgcgag ctcggtggtg gcaagctctg ggtgcgcggg ttgggcagtg acgtgagcgc
    17221 agtcgaggtg cgcttcgggc tcgcgcccaa ggtcggcttg gccaagaaag aagggctaga
    17281 cagcaaaccg cacgcccacc tcaacgtgtt gcactgcccc atgactgtac cctcggggcc
    17341 tcccctgcgc gatgtggacg gcgcgcgttt ggtggtgcgt ttggacgagc actgcgcgcg
    17401 caacctgaac gccctcacct tcgaagtcga cgatagaccg gtgcgggtgc tgcagagcca
    17461 ctcggacgcg cgcgagacct tcgtactgtt ggacgtgggc cgcttggacc gagacgcaat
    17521 cgcgatccga gctcgaccga agcaaggaga cgatgacgac gtcgtcgccc tcacgcgcgt
    17581 ggacacgatg aaacccccgc gcaaccgctc gagcctggag atccccggcc acaagaacct
    17641 ggatttcatc ccgaacaatc gccccgcccg cgtacacgtg gctccagtcg gagccccggg
    17701 gcggcttgcc gtggtgcccg tggaaggcgt gtaccacgtg ccctccgatg cgcccgacca
    17761 gattgtgggg gaccccgacg cagccgggct cgtgacctta cgttttgcct accgagtgga
    17821 gacgctgccg gaggaattca gaaacgtgga cctcgcggtc atggatgatg ttctgcagcg
    17881 cagcgttcat gaagcgaact tgcccgtgcc cctgggcggc tccgtatttg gaccgaaccc
    17941 actcgtcgaa ctcgtgtgcg gccccacgct tcacggcaag cgcgtgcaac ccggagagac
    18001 cgagcggctg ccgttcacca gccgcgatga ctgtcgcttg gtgttccatc gcgagcgcat
    18061 gccgcgcgac tacggcagtc agaaactcac gctcaacatc gaagtcgtca acccggatgg
    18121 cgcagtacgc cccgacgggc gcgtcgcgca gaccctcacc ctgcggcccg gtcgtgtccc
    18181 cgtttacgct tggatccacg gcgttcagaa cgaattcgac cgcgtcatcg tccggctctc
    18241 ccacgccgct gacgaggctc attacatcgg cgccagcgaa atagacaccg gctcacctgc
    18301 cgtgaaatgg acggtggtat tcggtcgagg ccgcgctcgc ctctacgcca ccaccgcgat
    18361 cccaaccggg ctctaccgtt ttggtgaccc ggctcacagc ggactattgt ctctgaactt
    18421 cggcgtcgtt tcgcgtctaa cctggctcaa cgaagatggg cgcgaaggct tcttggggct
    18481 cgagaccgga atcatggcga tcggactcgg caacgaccgc agcgccaacg gcgagtcgct
    18541 cacccaaatc ggcgccgtcg gcgggctcgg cgtggccgtc cccatcgcca atcgctccgc
    18601 gcccacccaa gcctcgatca acctgcacgg ctgggtcgag gtcgacatct cccacaccga
    18661 gggcaatcgc ttcgccttca tcttggggcc cagcgtgtcc attggcaatg tcggcgcgaa
    18721 cttgtgaggc gtcgagacgc gatcgcaagc ctcggtcacg gtttgccggg agcgcaacga
    18781 cggtattgca aaagcaaatc cttccgcacg taaagcgggc tactcgagcg actgacgacg
    18841 cgagcgtagt tgcgtaagca ttggcctccg cggatctccc gattcagagt cacgtagttc
    18901 ttgtgcggca ggaagatcgc ctccggatcg tcttcctcac acagctgatc gaagctcttg
    18961 gagccccgaa atagccaacg tggactgagc agccctgcca gatcggtcac cttaccgttc
    19021 tggaagcgca acccaaccac ccccacttca gagtgataga tatgcaggct gtcccctaga
    19081 cacttcacca acgcgtccag cccgcgataa gtggttacgt tgctcgcgtg ccgcgtcagg
    19141 taggcatcgt cgttcaaaag ctccggcttt ccgagattcc aactcgcgat ggcggtaccc
    19201 agcgcgagtg cggctgggac cagggggcgt cgcaccgccg tcaccaccgg ccagctcagt
    19261 acactcccaa acaagataca actcacgatt aagctcggcg gcacctgatc catgaaccaa
    19321 tcgtccgccg ttggcacgaa gtgaaatcga agcaacaagg cgacccctgc cgcgtaggag
    19381 accagacgca cgcaacgcaa gtacccgcgg ggcacgagcc agctcgcctt ggcctcactc
    19441 tccgccttta cgcccgcgag cccgcgagca cccgcaaaaa ccagtagagg caacgctgga
    19501 gcgtagaatc gcccctgcat tcccattaca tcgatggtgc ccgcgaggtg gtagagttcg
    19561 aacagcgccg cggtcgcgag cagcgtccag ttcatgcggt cacgccgcaa tagcgcgccc
    19621 aacaccagcg gcagcgcgac gaacgccaat gtaccgaagc gaatccgctg cacctctcgg
    19681 gagagcgctc gaaagtggtc gtcgtacggc gaaaaggcgg ccgtcttcgc gtaaaaaggt
    19741 agcggcagca ccgttccata taccagcttg aaaagcgcca agaacgtccc cactcccagc
    19801 aacacaatca ggagctcacg ccagggaagc acctttgtcc gcgcatagcg ttcaaccagc
    19861 aaaggcggta gcaccaacgc caaagcgtcg ggacgagtca cgtagagcag aaccgcgagc
    19921 agcggcgcaa tcgcgaagct ccatcgtctg acgcgcgtag ggttcacgcc tgggctgtcg
    19981 tttgccccgt aaacgagaca aagaatgacg gaggtgatga ataacgcgag cggcgtctcc
    20041 ataccagaca gcgccatgaa caagcacacc ggcgaacaaa ttgcgtaggt tgcagctaag
    20101 cccgaatctc gttctcgcgt cccgtagagg agtacggcgt gagtggccag cagcagagcc
    20161 gccgaaatca cccgcatggt tagaacgaag tagtcccgga aggcgcgcgc agccagccag
    20221 gccacccact gccaagtctg tgaggtcaat ccgtatacgg ggccctcgtc gacgttccag
    20281 gccaagctcc cggacgaatg aagttcacgg ccacgcgctt gaagaaatac gaatcatcat
    20341 agggctcacg ccccaacatc ccccaaacga agactgcgag cgcaacggcc acgcaaactc
    20401 gcagccacac catcggacgc tcgaaccggc gcatttgctc gcaaactagc ccgccgcgcc
    20461 gagcaacgtc cagcgcaacc tcgctgtagc cagccgaaaa ctcgagctcg cggcgagtgc
    20521 gtgcgacccg gagagg
//
LOCUS       DASTKU010000482         9297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_983100, whole genome shotgun sequence.
ACCESSION   DASTKU010000482 DASTKU010000000
VERSION     DASTKU010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983100"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFQ61_16690"
     CDS             complement(<1..248)
                     /locus_tag="VFQ61_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HET9956148.1"
                     /translation="MTDNTVLPQIATHPPVTPRAAVPHIMASHTIASRTEWLARRREL
                     LEQEKEMTRLRDQLSARRRELPWVRVEEQYVFQGPKGA"
     gene            532..1776
                     /locus_tag="VFQ61_16695"
     CDS             532..1776
                     /locus_tag="VFQ61_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9956149.1"
                     /translation="MDSQLNAAALALSRGDALSALKRVALREDPGALALRGIAFAQLG
                     ELARARDLLRRAGRGFGPREPKARARCLLAAAEVALAARDLGTSGKLERDLTRARRAL
                     EQQGDLANAAHAYSVEARHSLLIGQIPAAERALDALDVLLRKLPHKLPMLEAIAALLR
                     AQVAVRTLRCRAARLALDRAALAAHESRIAALAAEIETVRAALQVPAARIVLANGTEP
                     LVLEQVETLLLSSALIVDACARVIRHREIVISLQRRPVLFALARLLAEAWPADVPRDQ
                     LVSGAFGIRFGNDSHRARLRVEIGRLRRALEGVATLRATNAGFRLEPSRAERVAVLAP
                     PFDHEHAALLALLADGQAWSSSALALALGASQRTVQRALLELERTRATRSFGRGRARR
                     WLIPPLSGITTTLLLPSPLGHG"
     gene            1788..2474
                     /locus_tag="VFQ61_16700"
     CDS             1788..2474
                     /locus_tag="VFQ61_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine cyclotransferase"
                     /protein_id="HET9956150.1"
                     /translation="MKQSHRETARSSSADSESTAEIVREYGPFSGAPQVHGLTFDGRQ
                     IWFAAGDHLQSFSPENGQELRTIEVAAHAGTAFDGRYLFQIADDRIQKIDPETGRVVA
                     TIPAPAQGRDSGLAWAEGTLWVGEYRNRKIHQVDPETGKILRTIASDRFVTGVSWADG
                     ELWHGTWEGDESDIRRVDPETGAVRERLELPAGTELSGLESNGADLFFCGGGPSGKVR
                     AVRRPKRKSV"
     gene            complement(2510..3496)
                     /locus_tag="VFQ61_16705"
     CDS             complement(2510..3496)
                     /locus_tag="VFQ61_16705"
                     /inference="COORDINATES: protein motif:HMM:NF019468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HET9956151.1"
                     /translation="MTTSNRPLLARDEMLTWLIYALLALGFVLGISPLLIAAPRLPEF
                     VAVHWSLAGLPDGSMPKRYVLFLPPLLLGPAVVLNWISARASAPSRARLVLPLAMFSA
                     ALCGVLGTVLAAANWGRAHWQSAAHLAPSVAVSVVALPLVFALGTLGVAVRRWPASPH
                     SLLPPGPKLPLSGQDRAYWSGSASNRAMLLLLLLGAPVMLLVAEQRDALWALGITHVL
                     CFVLLELLTKIRVTVGEREVRIRYGALGWLSSRLPLDEILEANALELSPLDFGGWGYR
                     WCRGKRTAVVVRAGLALRVALRSGRQWFITVDDAEAAASLINGLIERKRRSG"
     gene            complement(3537..3884)
                     /locus_tag="VFQ61_16710"
     CDS             complement(3537..3884)
                     /locus_tag="VFQ61_16710"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET9956152.1"
                     /translation="MLIRVQPQDPTPLYTQIAGQVRRAVMEGRVKPGERLPGARELAD
                     ALQVHMHTVLRAYDELRQEGLVEVRRGRGVVVLANGPDRSRLLELARALRAEGARQGL
                     ALRELRKLLGEVG"
     gene            4157..4822
                     /locus_tag="VFQ61_16715"
     CDS             4157..4822
                     /locus_tag="VFQ61_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9956153.1"
                     /translation="MSIMQTARVQTESALGSNCAPQTSAASQPNVWLELRHRGQMLVF
                     ELTDGGRPLMIGSGWCSDVRLDGIGVALVHVQIYLENGTLWLAPHSAAHVRLNAAHVG
                     RLRALASHSVIEFLSYEIEIWVHDRVPHPVTGLASVDAHTERAPETLRGFAAPEHDPL
                     EAGYTPVAVVAHTIQNIEPRTEAPCATPVPIRTHAIELHGTNMVSWLPIVPMRCMLAA
                     LSD"
     gene            5014..6540
                     /locus_tag="VFQ61_16720"
     CDS             5014..6540
                     /locus_tag="VFQ61_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956154.1"
                     /translation="MPKDLQHHHPLVVGLRQLRSWSTPSALACAALLGCSSTESGSAG
                     SGGKAGQGGAATTGGATLATGGTGTSAGAPGTGAGGAGAGGTTSGNSSGGVSFGGSSS
                     GGASSIGGTGVTTGGSSNAAGNAGATGGVNADGGTSGAAGTNTAGNNNGGSATGGTLS
                     GGSGGQSGGGTAGQMAGGAGAGGQTSGGDIPKYFSSLPCGAKYTALGDGGWRFCVRLE
                     DGGGACANRNEVFERITFSGGGAITNVAQVSGYGDGPVAVVTTAGALHTGSSATSINT
                     TPVIASGVVNFSGGLHSRVALVRQGQGFSVVGWTENGSPAPIALPSGVQPVQVSGNYG
                     LACALATTGDVYCWEAGGNHSLGFTTTPSKAQLGKPVKLISVGQNTVCGVSFDNTLEC
                     QVAWYDSPWVPTEGSAPNFKVRQTTFPSIAEVHAGYHQGIIVRSDGAAFFLEGNSPGM
                     DNPGKQFTGATNVVAAGGDRGNACVQAGDGSVYCFVNGAVKQATLSGAPLKVKAATCP
                     "
     gene            6957..7238
                     /locus_tag="VFQ61_16725"
     CDS             6957..7238
                     /locus_tag="VFQ61_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956155.1"
                     /translation="MLDRGEVSTRAELATLAELSPVRVSQILGLLRLHPAILAHIDGL
                     SPGVPDEYLAERWLRPIARLDHAKQLDAVVGRLRWAVDQDDADRITGQT"
     gene            8960..9274
                     /locus_tag="VFQ61_16730"
     CDS             8960..9274
                     /locus_tag="VFQ61_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956156.1"
                     /translation="MPEKDEIARVLARAHRDVEPTITRIVRIVGPNEADGGEPIKLLE
                     VNSAAVASGIWPIAFTPDPPDIPYGSVVVEVTPDEYAAIVTDRLTLPPGWTLGDQLFA
                     AA"
BASE COUNT         1768 a         2982 c         2940 g         1607 t
ORIGIN      
        1 tcggcgcctt tgggcccctg aaagacgtat tgttcctcga ccctcaccca gggcagctcg
       61 cggcgccggg cgctcaactg gtcgcgcagt cgagtcattt ccttctcctg ctccagcaac
      121 tcacgacgtc gagcgagcca ctcggttctg gatgcaatgg tgtgtgaagc catgatgtgt
      181 ggaacagcgg cgcgcggagt caccggcgga tgagtcgcga tctggggcag cactgtgttg
      241 tcagtcatga aacggatgta ctgccgagcg agggttcggc gtgagtgaca agcttggcgc
      301 gattgtcgga tccgcgggct ggctcgggac cggcgggtat tgacctgaat ttcgcgccga
      361 cgacggacga gatcggcaag accgaagcga gatcggcttc agattggctg ccggcgcgcg
      421 cagccatagc agggctatgg cgagcacgtc ggcacccaag atgaggctgg aagcgcgagg
      481 aattgcccgc ttcgaactag gaggcacgaa attgaggtct aaactgatgc catggactcg
      541 cagctcaacg ccgccgccct agccctgtcg cgaggggacg cgctgtccgc gttgaagcga
      601 gttgcactcc gtgaagatcc gggagcactt gctttgaggg gcatcgcctt cgcccaactc
      661 ggggagctgg ccagagcgag agacctgctg cgcagagctg gacggggctt tgggccacgc
      721 gaacccaagg ctcgcgcgcg ctgcctgctc gcggcagctg aagtagccct ggccgcgcgt
      781 gacttgggaa catccgggaa gctcgagcgt gacctgacca gggcccggag agcgctcgag
      841 cagcagggtg acctggccaa cgccgctcat gcctactccg tggaggcacg ccactcgctc
      901 ctgattggac agattcccgc cgcggagcgg gcgctcgatg ccttggatgt tctgttgcgg
      961 aagctacccc acaagctgcc catgttggag gccatcgctg cgctacttcg cgcacaggtc
     1021 gcggtgcgca cgttgcgttg ccgagcggcg cggctggctc tcgatcgggc cgcgctagct
     1081 gcccacgaat ctcggatagc ggcgctcgcg gcggagatcg aaacggtgcg ggccgccctc
     1141 caggtccccg ccgcgcgcat cgtattggcc aatggcacgg agccactggt actcgagcag
     1201 gtagagacgc tgctactttc gtccgccttg atcgtggatg cgtgcgcgcg ggtcatccgt
     1261 catcgtgaga tcgtgatttc tttgcagcgt cgtccggtcc tgttcgcgct ggcgcgctta
     1321 ctggcggagg cttggcccgc ggatgtcccg cgagaccagc tggtgtccgg cgcgttcggc
     1381 atccgcttcg gcaacgattc gcatcgcgcg cgcctgcgcg tcgaaatcgg tcgattacgc
     1441 cgtgcgctcg agggggtggc caccttgcga gcaaccaatg ctggatttcg cctcgagccc
     1501 agtcgggccg agcgcgtcgc cgtgctggcg ccaccgttcg atcacgaaca cgcggcactg
     1561 ctcgcgctgc ttgcggatgg tcaagcttgg tccagttcag ctctggcgtt ggcactcggt
     1621 gcgagccaac gaaccgtaca gcgcgcgctc ctggagctcg aacgaactcg cgctacccgc
     1681 agctttgggc gaggtcgcgc gcgccgctgg ctgatccctc cgctctctgg aatcacgacc
     1741 accttgttac tcccctcccc tctcggccac ggttaacctg ttctgacatg aaacagtcgc
     1801 accgcgaaac cgctcgctcg tcttcagccg actcggagtc aaccgcagag atcgtccgtg
     1861 agtacgggcc cttttcgggt gccccgcagg tccatggcct caccttcgat gggcgccaga
     1921 tttggtttgc cgcgggggac cacttgcaat ccttcagtcc cgagaacggg caagagctgc
     1981 gaacaatcga ggtcgcggcg catgccggca ccgcgttcga tggtcgctat ctctttcaga
     2041 ttgccgacga ccgaattcaa aagatcgatc ccgaaacggg ccgcgtggtc gccaccattc
     2101 ccgcgccggc ccagggtcgc gactcgggat tggcttgggc ggaaggcacg ctttgggtgg
     2161 gcgagtatcg gaatcgcaag atccatcagg tcgatcccga gacaggcaaa atcctgcgaa
     2221 ccatcgcctc cgatcgcttc gtcacgggcg tgagttgggc ggacggtgaa ctttggcacg
     2281 ggacgtggga aggagatgaa tcggatatcc gccgagtcga cccggagaca ggtgcggttc
     2341 gagaacggct cgagttaccg gccggcaccg agctcagtgg gctcgagtcc aacggcgcag
     2401 atctgttctt ctgtggcggc ggcccgagcg ggaaggtgcg ggcagtgcgc cgcccgaagc
     2461 ggaagtctgt ctaacgcggg tggttcggga tgtcgttcta ggctgcagct cagccggagc
     2521 gccgcttgcg ctcgatcaac ccattgatca ggcttgccgc ggcttccgcg tcatcaacgg
     2581 tgatgaacca ttgtctgccg ctgcgcagcg caacgcgcaa cgccagaccc gcccgcacga
     2641 ccaccgcggt ccgcttgccg cgacaccagc gataccccca gccgccgaaa tcgagcggag
     2701 agagctcgag cgcattcgcc tccagaatct cgtccaacgg cagtcgggaa ctcagccagc
     2761 ccagggcgcc atagcggatg cgcacctctc gttcccctac cgtaacgcgg atcttcgtca
     2821 gtagctcgag caggacgaag cagagaacgt gcgtaattcc caatgcccac aacgcatccc
     2881 gctgttccgc gacgagaagc atgacgggcg cgccaagcag tagcaatagc agcatggccc
     2941 gattcgaagc gctgccgctc cagtaagccc gatcctgccc agagagcggc agcttgggcc
     3001 cgggcggcag cagagagtgc ggggaagctg gccaacgccg aactgcgacg cccaaggtcc
     3061 caagcgcgaa caccagcggc aaagccacca cggaaaccgc cacactcggg gccaagtgcg
     3121 ctgcagactg ccaatgggcc cgtccccaat tcgcggccgc cagcaccgtc cccagcactc
     3181 cgcacaaggc ggcggaaaac atcgctaacg gcaagaccag gcgtgctcgg gacggcgccg
     3241 aggcgcgcgc ggagatccaa ttcaagacaa cggccggtcc gagcagcaga ggcggcaaga
     3301 acaaaacata tcgcttcggc atacttccat cgggaagacc agcgagcgac caatggaccg
     3361 ccacgaactc gggcaaacgg ggcgccgcaa tcagtagcgg agatatcccc agcacgaagc
     3421 cgagcgcgag caacgcatag atgagccacg taagcatctc atcacgcgcc aggagaggtc
     3481 ggttggaagt cgtcatgcgt tcctcttttg cgtttcgagt gggctcgggc tgcaagtcag
     3541 ccgacctcgc ccagcaactt gcgaagctcc cgtagcgcca gcccctgacg cgcgccctca
     3601 gcacggaggg cgcgcgctag ctccaaaaga cgcgagcggt ccggtccgtt cgcgagtacc
     3661 accacacctc ggccgcgcct cacttcgact aacccttctt gacggagttc atcgtatgcg
     3721 cgaagcacgg tgtgcatgtg aacttgcagc gcgtcagcga gctcacgcgc gcctggcagg
     3781 cgctcacctg gtttgaccct gccctccatc acggcgcgcc gcacctggcc cgcaatctgt
     3841 gtgtagagcg gcgtcggatc ttggggctgc acccggatga gcattgttat attcgtaata
     3901 taacaatgct cactgtcaag ccgccccgct ttggttctcg actcaacggg cacggcggga
     3961 tgctttcgtc ccctacccga gtcgtccggg agcgttgacc cgagcgcaac cgcgcgaccc
     4021 tgacgaccga aaaatgccca ggctaggtgt gacaatcaaa gtccgcgcac ggcgcctcca
     4081 cagctgtttt gacggcagaa atcgtcccgc ttcgcgtata ctcgatcatc aagggacaac
     4141 gaaacgggag gcattcttga gcattatgca aacggctcgc gtgcaaacgg agagcgcgct
     4201 cggctcgaac tgcgcgccgc aaacctcagc cgcgtcacag cccaatgttt ggctggagct
     4261 gcgccatcgc ggtcaaatgc tggttttcga gctaactgac ggaggtcgac cgctgatgat
     4321 cggttcgggc tggtgctcgg acgtgcgcct tgacggcatt ggcgtagcat tggttcacgt
     4381 ccagatttac ctggagaacg gcacgctttg gctggcacct cattcggccg cacacgtgcg
     4441 cttgaacgca gcccacgtgg gtcgccttcg cgcgctcgcg tcgcactcgg tgattgagtt
     4501 tctgagctac gaaatcgaga tctgggtgca cgatcgagtg ccccacccgg taactggact
     4561 cgcttcggtc gatgcgcaca ccgagcgcgc accggagacg ctgcgtggct ttgcggcgcc
     4621 cgagcacgat cccctcgaag cgggttatac gccagttgcc gtggtggccc acacgatcca
     4681 gaacatcgag cctcgcaccg aagcgccgtg tgcgacacca gtgccgattc gcacgcatgc
     4741 catcgaactc cacggtacca acatggtgag ttggctgcca atcgtgccga tgcgctgtat
     4801 gctcgcggcc ttgagcgact gaccaacacc atagggctga atcccaagga tccgcgcccg
     4861 agcgcgaggt gtcctgggtg acctcgcccg catcctgcac cagtaaatct cgccgcaaac
     4921 gcgaccgagc gccgcccaat ggcccgcgtg cgctttgcgc gaagccgctt tctgacgcat
     4981 acctctccag ctcgggacga gcggagcgca tgaatgccga aggatctgca gcatcatcac
     5041 cctctggttg taggattgcg tcagcttcga agctggagca caccgagcgc gcttgcatgc
     5101 gcggcgctgc tcggctgtag ttccacggaa agcggctccg ccggaagcgg aggcaaagcc
     5161 ggtcaaggcg gcgccgcgac gacgggtggt gcgaccttag caaccggcgg gaccgggacc
     5221 tcggcgggcg cgcccggaac gggcgctggc ggtgcgggcg cgggcggaac cacgtcaggc
     5281 aactcgagcg gcggtgtttc gttcggtggc tcgtcttccg gtggcgcgag cagcatcggg
     5341 gggacgggag taactaccgg cggctcgagc aacgccgcgg gcaacgccgg ggctacgggt
     5401 ggcgtcaatg ccgacggcgg aacgagcgga gccgcgggca ccaacaccgc cggcaataac
     5461 aacgggggat cggctacggg tggcacgtta tcaggaggta gcggtgggca atccggaggc
     5521 ggaaccgctg ggcaaatggc gggcggcgcg ggtgccggcg ggcagaccag cggcggcgac
     5581 attccaaagt acttctcgtc cttgccgtgc ggagccaagt acaccgcact gggtgacggc
     5641 ggctggcgtt tctgcgtgcg gctcgaggac ggtggcggcg cctgcgccaa tcgcaatgaa
     5701 gtcttcgagc gaatcacttt ttcgggcggc ggcgcgatca cgaacgtcgc gcaggtgagc
     5761 ggctatggag atgggccagt agccgtagtc acgaccgcgg gagcgctcca caccggctcg
     5821 tccgcgacct ccatcaacac cactccggtg attgcgtcag gagtcgtgaa cttcagcggc
     5881 ggtcttcact cgcgcgtcgc gctcgtgcgg cagggccaag gcttctcggt cgtaggctgg
     5941 accgagaacg gctcaccggc gcccatcgca ttaccttcag gggttcaacc ggtccaagtc
     6001 tctggcaact acggtttggc gtgcgcgctt gccacgaccg gcgacgtcta ctgctgggag
     6061 gccggcggca accacagcct cggcttcacg acgaccccca gcaaggccca gctcggcaaa
     6121 ccggtcaagc tgatctcagt cgggcagaac acggtgtgcg gcgtcagctt cgataatact
     6181 ctcgagtgcc aagtcgcttg gtacgatagc ccctgggtcc ccaccgaagg ctcagcgccc
     6241 aacttcaagg tccgccagac cacattcccg tcgatcgccg aagtgcacgc gggctatcac
     6301 caaggcatca tcgtccgcag cgatggagcg gcgttctttc tcgaaggcaa ctcacccggc
     6361 atggacaacc ctggcaaaca attcaccggt gcaacgaacg tcgtggccgc cggcggtgac
     6421 cggggtaacg cctgcgtcca agcaggggac ggctcggttt attgcttcgt caacggcgcg
     6481 gtcaagcagg caactttgag cggcgcaccg ctcaaagtaa aagccgcgac ctgcccctaa
     6541 ctgacgcgac tccgggggag cgcaccgcca gcgacgtagg ccgttcgctc ccaggctgag
     6601 tctcgtgcgt ttcgaacggc tcgttcgtct ggacgaacca atcaccgtcg tacgcagcgc
     6661 ctgtatggtg gacgaccggc aacacccgat cgagctcccg ccagcctgaa tgctattggc
     6721 gcgcccggga agaatcggcg cagaagctcg cgcacggcgc gagcggacag attcatcttc
     6781 ttcaatcttc acgggtcgat aagcccggaa ggggttggcg ccccaatagc gacgaaagta
     6841 agaacttaag gctcgtggtt ccgaacccgg ttcgaagggc ggatccacct ccgacttcgg
     6901 aaccccaagt tccgagaatt atgaagcttc tcgcgctggc tcgcgagtgg caagcgatgc
     6961 ttgatcgtgg tgaagtcagc acgcgagccg agctggcgac gctggccgag ctctcgccag
     7021 tgcgagtttc gcagatcctc ggtttactca ggttgcatcc ggcgatcttg gctcacatcg
     7081 acgggctgtc gcccggtgtc cctgatgagt acctggccga gcgctggctg cggcccattg
     7141 ctcggctgga tcacgccaag cagctggacg ctgtcgtggg acggctacgc tgggctgtcg
     7201 atcaggacga tgccgaccgc atcacagggc aaacgtaatc ggcccgcgcc tacctggacc
     7261 atcttgagta gcggacccgg cattagccga gggtcatccg cgagctgccg gacagcggcc
     7321 gatggccagc gacattcgtc aaacgtcgct caacggtggt cagcgaatga agggatttgg
     7381 aaaacccgac gtgcttgcag cgatgatggc gagaatgaca accacacgtc cacacggcgg
     7441 caagagactt agaacaagaa ccagcaaccc caacgggcgc atcccggata cgcgcagcac
     7501 gcgagaactg aatgcttgct ttggacattc ggacattctg ctgatgctgc ggttcaaaca
     7561 tcgagctggg acccgatccc tctcccccac ggcctaaacc aagccaatcg ccgcaatccc
     7621 agaccagcct aatcccatcc gctttaggac ggcgccgccc acccggaccc gtcccgaatc
     7681 gcccgagtgt cgcaagctcc ccgtcgtcga aaacgctttc cgccgcggga gagggctgca
     7741 cagcccgcag ccttctcccg ctcgacagct ggcgcaaagc ggtgcagggt gcgggtgaaa
     7801 caagagttgg gcggacgaag ctagccatct ccatcgacat tcatcagagc atcgtagatg
     7861 accaaaccgc gcgtcaccaa ggtcgtccca atcggatctt tcgcacttac actggtatct
     7921 gccgcgtctt gtcagccgcc acgtgtgccg ccgcctcagt gccctccgcg tgtttgcccg
     7981 gtgggtggag tcgcgaggga cgttagttcc gcaacctccg gaatgaacat cacggctggt
     8041 ggagggacca cgatcgcagg cggtcaagct caggccagtt tcgccatata tggtcaagcc
     8101 gcgtgcagct acatgtgcac tttttcgaca tgtccggtgg gaaccgcacc acgcattact
     8161 cgaggcacgt tcgaatgtta tgatccctgt tccggaatcg cgtgtcccgc cgggacgtcg
     8221 ccgaatgaga cgagcgttgc ttcggttgta accggcacgc ctggtcccgt gactgccaaa
     8281 gcggtcgatg gccgcgtgga ggttgggaac gccgaatgca agcgcaagtg cgatgtgatt
     8341 caaccatgcc ccgcggacac gaggccggtg atcacgcccc agaagtatga atgcgtgccc
     8401 ctgccgcctt gtgataacgg ccggcaccgc gatacatcgg ggtactgtgt accagatcca
     8461 ccccctccgc cgccgagcca aacccgctgc gaggtcaggg attgcggatt cacaaacaag
     8521 acgaactgct cggtgacgtg tgactccccc cggcaggcgc gatgctcgtg cgatggttgc
     8581 ggcttcctct gcgcgcgaga caattgcagg tgcgaatgac tggatggacg gcggcacaat
     8641 tgccaagcga atcgtcgaac tcgacgccca gctcttcgaa ccgccagcat tggcatccgc
     8701 atcgcgaggg cgtgtaacca acgggcagaa gcgccgccca acaagctgtt gaagctgacg
     8761 gccgcgcctc gtcgtgctct gaaccgtcct acttctcacc cgtgcaaatg gcacgggctc
     8821 ggtcatcgct tcctgatacg tcctccgttt ctgaatgcgg ccgcagctta acagcggtac
     8881 gttgggcaga ccggcatgcc cgccgaagcc ttccgtagac actcttgccg gagtggggcg
     8941 gcacgcgtac gctacgtcta tgcccgaaaa ggacgagatc gcgcgagtgc tggcaagggc
     9001 gcatagggac gtcgaaccga cgattacacg catcgttcga atcgtcgggc caaacgaggc
     9061 cgatggaggc gagccaatca agctgctcga ggtcaacagt gcggcagttg cctctggcat
     9121 ctggccgata gctttcactc cggacccgcc ggacatcccc tatggatcgg tggttgtgga
     9181 ggtcaccccg gatgaatatg ccgccatcgt tacggaccgc ttgaccttgc ctcctggttg
     9241 gactcttggc gatcaacttt tcgctgcagc atagctatgg ccacgttcgc ggaggcc
//
LOCUS       DASTKU010000483         9207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1877922, whole genome shotgun sequence.
ACCESSION   DASTKU010000483 DASTKU010000000
VERSION     DASTKU010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877922"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..455)
                     /locus_tag="VFQ61_16735"
     CDS             complement(99..455)
                     /locus_tag="VFQ61_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956157.1"
                     /translation="MSIAQYLMAGALCLGCAASPSEARAPQPPTASLPPGQARFLCQT
                     EGQCSQAALARCGNRYEVIRKARVQESEPPKGVAVQSNGTRWEWIRDKNRKIYTPRKP
                     LPDLEMVVWCPAQTAS"
     gene            complement(550..2970)
                     /locus_tag="VFQ61_16740"
     CDS             complement(550..2970)
                     /locus_tag="VFQ61_16740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9956158.1"
                     /translation="MADVDMSAVGVSQARLPLALRGVLPALERSPDGIIICCRREHGR
                     YATTYANATFREWVSQRLGSSEVSELNIEIELEDRRVPLDAFLERGADAELDTFEGFG
                     WIVTRGAGGRCRVRVVRDNVDGECLATLHWRWPRVDLDGTRTELVLIEEAGLRPIASP
                     SIRDVLGWGNPVQTGDGSDSPAAPGWGPLELAHPADIERVREVRAWLEHAPLGKAVSV
                     LFRARHGSGEYRWLGGTARVAQGYFGHAWVWNLLDVSELKISQEELRGTRALLEQAQS
                     VARIGTFRWALLNPGLMECSPEGERVLGVSSATELSASSFFSLCHDEDMPSVYAAFDK
                     AGQEGGHFELECRLLGRDGTLRWVRVFGGVAYDSMTGQALELIGVLRDVSEQKRIFAQ
                     SFESQKMEALGRLAGSVAHDFNNMLSAIQGFARFIHDDAPDDSSVREDAAQILRASAR
                     AASLTQELLAFGRRQVLEPRVLDVREQIRDMEKMLTQLLPEHAKLRIVLSNDLNRVKV
                     DPVKLEQVVLNLVLNAREAMPRGGTITIETSNVCLHDGYARSHVDLRPGNYVMLAVTD
                     TGVGMTEEIRNRIFEPFFTTKEAGRGTGLGLATVFGIIKQSGGHVNVYSELGRGSVFK
                     VYFPSCGEAPVREAPPTPISEAIALGETVLVVEDEGSVRELVQRILQRSGFGVLAAAT
                     PHEALELEESHRGRIDVLVTDVVMPGMSGHDLAVKLRRRRPELATVYMSGYTEHTVAD
                     QGVHLDGQWFVPKPIDPELLLGKVQEAARSLQREGRLRSEMPASGAVSLASPIGVTHE
                     LGLGKASA"
     gene            3401..5962
                     /gene="hrpB"
                     /locus_tag="VFQ61_16745"
     CDS             3401..5962
                     /gene="hrpB"
                     /locus_tag="VFQ61_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HET9956159.1"
                     /translation="MTTLPIDEVVPSIVESLGRHPTLVLEAPPGAGKTTRVPWALLGS
                     QSVGEVVVTEPRRLAARMAAQRVAHERSVKLGALVGYRVRFEELVSRATRLVYMTEGV
                     LLRRLMTDPELRGVASVVLDEFHERHLETDLLLSLLSRLQKGARPDLRIVVMSATLEA
                     EPVAAFLDDCPRIRSEGRVFPVEIEHLDAPDERPLEKQVVSAVRKLTLAEASGDVLVF
                     LPGAAEIRKAQQALETLAGEHRLLVVPLHGDLPVQEQARAIEPQVQRKVVLSTNVAES
                     SVTIEGITGVVDSGLARVAGHSPWTGLPTLETARISRASAVQRAGRAGRTRPGRVLRL
                     YTRGDFQSRVEHDLPEVARADLTDAWLLLHGSGIEDPSRMAWLTPPPAARAQAARELL
                     TRLHALDDAGHLTSIGRRMLELPLHPRLSRVFVEGERRGVAGEAAILCGLLGERDPRL
                     AQRSSFGSRRGPSDTATGPSDLLELLDVYELARALSFDAQRLRQHDLDARTLAAVKRA
                     AEQLARLTRPVAVDNSEDLETSLLICILSGFVDRVARRKRPGSDELTLQTGQSARLSP
                     ESVVRNDELLLAVDVEERRNKSMGDIQVRLASRVRPEWLLDLYPEALTLTEELAFDSE
                     RQRVERSSRLAFGAVVLEESRGAASPSAETTRVLCEAISSRGREEFLASTELSETLER
                     VALVARRYPKLGLPAVSLDDAKGLLFPLCEGLVSFEEVRRLDLVAELLSRLTPEQRRE
                     LDRAAPESIRLPSGRSARIHYEAGKPPWLESRLQDFFGLKEGPKLCAGSIDVTLHLLA
                     PNGRAVQVTSDLPGFWERHYPTVRRELMRRYPRHAWPEDAKSAPPIQPRPRRGDG"
     gene            complement(5976..8594)
                     /gene="mutS"
                     /locus_tag="VFQ61_16750"
     CDS             complement(5976..8594)
                     /gene="mutS"
                     /locus_tag="VFQ61_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET9956160.1"
                     /translation="MSGSKTPVMQQHAAAKRAYPDAIVFFRLGDFYEMFGEDAQLTSR
                     LLDLTLTSRNKGKPEEIPMAGVPYHAAHGYIAKLLELGHKVAICEQMADPSKVKGIVP
                     REVVRVITPGLLTDADYLNAHTNNYLAAVEFEPGASALALFDLSTGELSAAVLADATL
                     LLSALSRSRPREILLGFGKASDEERQTLLRALQALGAPLREDDALEAESVDEVLGDLQ
                     EEARRVGASGERAVARAMRFARRCNPGQTLPVTRVARFEPSENLAIDSTAQQHLELVE
                     SPAGRKATLLNVIDATRTPSGARLLRRRLLAPLLDVERIRRRLDAVEVFLARPTLREQ
                     FGEVLSAVGDLERLAVRASLGEATPRDLGALRDGLAAAAAAVESLQALDAREKEVLRL
                     IAPGSGDSGACEVDVVPELQAELARALVDRPPALAKEGSVFRSGFDSELDELDDLQKR
                     GAEKMVELEARLRQDTGVASLKVRYTRVFGWYIEVTRSQAAKVPEGFRRKQTVASGER
                     YTTPELDELSDRILHAEERHRDRELDLLKQLVALAGRHSERVRALAARIARWDVAQGL
                     AEIAHRHEYTRPVVDESDVITIREGRHAVVERLAEVGRFVPNDVDLASSGARLWLITG
                     PNMAGKSTFLRQVALITLLAQMGSFVPARSARIGVVDRILSRVGASDNLSRGESTFMV
                     EMRETAEILRSATRRSLVILDEIGRGTSTFDGLSIAWAVAEYLDEAIGCRALFATHYH
                     ELTGLAEASTHTVNYSVSAKEVGHDVVFLHRVGKGPASRSYGVAVAKLAGLPESVLSR
                     ARALLAMLEKDRRIDVGARRSPKSVPENQLGLFGANKQSEAKEREVLETLRNLDPDRL
                     TGLEALQLLARLKTRL"
     gene            complement(8596..>9207)
                     /locus_tag="VFQ61_16755"
     CDS             complement(8596..>9207)
                     /locus_tag="VFQ61_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956161.1"
                     /translation="AHACFPSATGYSIRVTNSWVLRSSAGFAHSIIAREERGADGTRV
                     FECRRDCNPRKRYFESRVFEITAPTDYCLNGSDKCRVGSDPNSPCVLPKPPSAQRQFE
                     NVVPFDVDRDAARKCIFESPTARFAIYRGQEESQEDMSFSWRTTGAFVPLAISMPSGS
                     GGIAPTALTHLPNLDRLSVVDASSLGIVFMSLDSISPISPTLN"
BASE COUNT         1621 a         3060 c         2845 g         1681 t
ORIGIN      
        1 cctctcgaat ctggcccgat tgacctggtc gatccgcggg cccgcgtggg cccgccgtag
       61 ctgactcaac gccgaatgtg cggcatgagt gagtgacctc aagacgcggt ctgcgccggg
      121 caccacacga ccatctcgag atctggcaga ggtttacgcg gcgtgtagat cttccggttc
      181 ttgtcccgaa tccactccca gcgcgtgcca ttcgactgaa cggcgacgcc cttgggcggc
      241 tcggactcct gcacgcgcgc cttacgaatc acctcgtaac ggtttccaca ccgtgcgagg
      301 gccgcctgcg aacattgacc ttcggtctgg cagaggaagc gggcctgacc ggggggcaaa
      361 gatgccgtcg gaggctgtgg tgcccgcgcc tccgatggcg aagccgcgca accgaggcac
      421 agcgcgccag ccatcaagta ttgggcaatt gacatgacca ggatgtagcg cgctccggcg
      481 tgatcggtag tgccaccgac gccggatgat tcaccccgga gccgcgcgcg ccggtgtcta
      541 acgtgcccct caggcgctgg ccttgccgag gcccaactca tgggtcaccc cgatcgggct
      601 tgccaggctc accgcgccgc tcgcgggcat ttcgctgcga agtcgccctt cgcgctgcaa
      661 tgatcgcgcc gcttcctgca ctttaccgag cagcagctcg ggatcgattg gcttgggcac
      721 gaaccactgt ccgtccagat gcacgccctg atccgccacc gtgtgctccg tgtatccgga
      781 catatacacc gtcgccaact cggggcgacg tcggcgcaac ttcaccgcaa ggtcgtgccc
      841 gctcatgccc ggcatcacca cgtcggtcac gagcacgtca atccgacctc ggtgggactc
      901 ctcgagctcc aaagcctcgt gcggcgtagc ggcggccagc accccgaacc cggaacgctg
      961 caaaatcctt tgcaccaact cccgcacgct cccttcgtcc tcgacgacca acacggtctc
     1021 gccgagcgca atggcctcgg aaatgggcgt tggcggagcc tcacgcaccg gagcctcgcc
     1081 gcaactgggg aaatagactt tgaataccga gccgcgcccc agctcgctgt aaacattcac
     1141 gtgtccgcca ctctgcttga tgattccgaa caccgtcgcc agcccgagcc ctgttccgcg
     1201 ccccgcttcc ttggtggtga agaacggttc gaagatgcga ttgcggatct cttcggtcat
     1261 ccctacgcca gtatcagtaa cagccagcat tacgtagtta ccgggtctca gatcgacgtg
     1321 ggagcgtgcg tagccgtcat gcaggcacac gttggatgtc tcgatcgtga tcgttccgcc
     1381 gcgcggcatt gcctccctgg cattcaacac caagttcagc accacttgct cgagtttgac
     1441 cggatctact ttcacccgat tcaagtcatt cgacagcacg atccgtagct tcgcgtgctc
     1501 gggcagcagc tgcgtcagca tcttctccat gtcccggatc tgttcgcgca cgtccagcac
     1561 ccgcggctcg agtacctgcc ggcggccgaa cgccaggagt tcctgagtga gggacgccgc
     1621 gcgggccgag gctctcagga tctgcgcggc gtcttcgcgt acgctcgagt catcgggagc
     1681 gtcgtcgtgg atgaagcgtg caaaaccctg gatcgcggac agcatgttgt tgaagtcatg
     1741 cgccacgctg cccgccaaac gaccgagcgc ttccatcttc tgcgactcga acgactgagc
     1801 gaaaattctc ttctgttcac tcacatcgcg gagcaccccg atgagctcga gggcttggcc
     1861 cgtcatcgaa tcatacgcga ccccgccaaa tacccggacc cagcgcagcg taccgtctcg
     1921 gccaagtagg cgacactcga gctcgaagtg gccaccctct tgccccgcct tgtcgaacgc
     1981 cgcgtagacg ctcggcatgt cttcgtcatg gcacagcgaa aagaacgagc ttgccgacag
     2041 ctcggtcgcg ctgctcacgc cgagcacccg ctctccttcc ggcgagcact ccatcaggcc
     2101 gggattgagc agtgcccagc gaaaggttcc gatgcgtgcc acggactgag cttgctcgag
     2161 cagggcgcgc gtaccgcgca gctcctcctg gctgatcttg agttcactta cgtcgagtaa
     2221 gttccagacc caagcatgtc cgaagtagcc ttgggcgacc cgagcggtac cacccagcca
     2281 gcgatattca ccgctgccgt gccgggcccg gaagagtacg gagacggctt tcccgagggg
     2341 cgcatgctcg agccatgctc tcacctctcg cacgcgctcg atgtccgccg gatgggcaag
     2401 ttcgagtggc ccccagccgg gagcagcggg cgagtcggaa ccatcaccgg tctggactgg
     2461 gttgccccag cccaaaacgt cgcgaatgga aggactcgcg atcgggcgta ggccggcttc
     2521 ttcgattaag acgagttccg tgcgagtccc gtcgaggtcc actcttggcc aacgccaatg
     2581 gagcgtcgcc aagcactcgc cgtcgacgtt gtcgcgcacg actcgaacgc gacaacgacc
     2641 accggcgccg cgcgtaacga tccagccgaa gccctcgaag gtatcgagct cggcgtccgc
     2701 gccgcgctcc aagaacgcat cgagcggaac gcggcgatcc tccagctcga tctcgatgtt
     2761 cagctcgctc acttcagaac tgccgaggcg ctgcgaaacc cactctctga atgtggcgtt
     2821 tgcgtacgtc gtcgcatacc gcccgtgctc ccgacggcag cagatgatga tcccatccgg
     2881 agagcgctcc aaagcgggaa ggacccctcg caaagcaagc ggcagtctgg cttgagacac
     2941 cccgaccgcg ctcatgtcga catcggccat gagctaacaa gcatacacgg atcgcaattg
     3001 cgtggtgaaa ctaccaattg gaatggtttt cgtggcgttc ttgacactcc tcacggtata
     3061 gcggcgccgg atttcgacag tcgttatggg tcgggtccgg tcccactgca tgctgacgag
     3121 tttcgcggtt accagccgaa aacccatgag tcctccgccg tttttccgtg ctcgtggcgc
     3181 gctgcgcggc gcaccttcga tacgttgttt cggcagtcga cagatcgaga gtaccgactt
     3241 cgaccaccgg tcgacctaca acctgcactc gtcgatatcc tgggctcgaa tacgttacaa
     3301 ataatcctgg ctcctcacgc gaactcttct tctcaagtct ctctccaccg aaaaaggccc
     3361 gttcttgcag cctccgctca ctccccccgg ccccagcgca ttgaccacac tcccaatcga
     3421 cgaagtggtt ccatcgattg tcgagagcct cgggcgacat ccaaccctgg tgctcgaggc
     3481 gccacccggc gcaggcaaga ctacacgcgt cccatgggcg ctccttgggt cgcagtcggt
     3541 gggtgaggtt gtcgtgaccg agccgcgccg tttggcagcg cgaatggcag cgcagcgcgt
     3601 cgcgcacgag cggagcgtga aacttggcgc tctggtgggc taccgagttc gcttcgagga
     3661 gctggtgtct cgcgccactc gcttggttta catgacggag ggggtgctgc ttcggcgctt
     3721 gatgacggat ccggagctgc gcggagtcgc gagcgtcgta ctcgacgagt tccacgagcg
     3781 tcacctggag actgacctac tgctgagcct gctatcacga ctgcagaaag gcgcgcggcc
     3841 ggacctgagg atcgtggtca tgagcgcgac tctcgaagcc gagccggtcg cggcgttcct
     3901 cgatgattgt ccgcgcatcc gcagcgaagg gcgagtgttt ccggtagaga ttgagcacct
     3961 cgacgcgccc gacgagcgtc cgttggagaa gcaagtcgtc agcgcggttc gcaagctgac
     4021 gctggcggag gctagcggag acgtgctggt tttcctgccc ggcgccgcgg agattcgcaa
     4081 agcgcagcaa gcgctcgaga cactagcggg cgaacaccgg ctgctagtgg tgcctctgca
     4141 cggtgacctc ccggttcaag agcaggcgcg agcaatcgaa ccccaagttc aacgtaaggt
     4201 cgtgctctcg accaacgtcg ctgaatcgag cgtcacgatc gaaggcatca ccggcgtcgt
     4261 cgattccggt ttggcgcgcg tggcgggtca ctccccgtgg acggggcttc cgactttgga
     4321 gacggcgcga attagccgag cttccgcggt gcagcgcgcg ggacgcgccg gtcgcacgcg
     4381 ccccggtcgc gtgcttcgac tctacacgcg tggtgacttc cagtctcgcg tcgagcacga
     4441 cttaccggag gtcgctcggg cggatttgac ggatgcttgg ctgctgctcc atggcagcgg
     4501 gatcgaggat ccgagtcgta tggcgtggct caccccaccg cccgcggcac gtgcgcaggc
     4561 tgcgcgagag ctcctgacgc gcctgcatgc tctcgatgat gccggccatt tgacgtcgat
     4621 tggacgtcgc atgttggagt tgcccctcca cccacggctc tctcgcgtat tcgtcgaagg
     4681 agaacggcgg ggcgttgccg gcgaagccgc catcctgtgc ggactactcg gagagcgaga
     4741 tcctcggctc gcgcaacggt cgagcttcgg ctctcgccgc gggccgagcg acaccgccac
     4801 gggaccatcc gatctgctcg agttgctaga tgtatacgag ctggctcgcg ctttgagctt
     4861 cgacgcgcag cgcttgcggc aacacgactt ggatgcgcgg accttggccg ccgtcaagcg
     4921 cgcggccgag caactcgcgc ggctgacgcg acccgtcgca gtcgacaact ccgaggatct
     4981 ggagaccagc ttgctgatct gcatcttgtc tgggttcgtg gatcgtgtcg cgcgccgcaa
     5041 acgaccgggc tcggacgagc tgacgctgca gacaggccag tctgcgcgtc tctcgccgga
     5101 gagcgtagtg cgcaatgacg agctgctact ggccgttgac gtggaagaac ggcgaaacaa
     5161 gtccatgggt gacatccaag tcaggttggc cagccgcgtg cgccccgagt ggctgctcga
     5221 cctttacccc gaagcgttga cgctgactga ggagctcgcc ttcgatagcg aacggcagcg
     5281 ggtcgagcgc agtagccgat tggcctttgg cgcggtggtt ttggaggaaa gccgaggggc
     5341 cgccagccca agcgcagaga cgacgcgggt gttgtgtgaa gccatctcga gtcgggggcg
     5401 tgaagaattc ttggcctcca ccgagttgtc ggaaactctc gaacgcgtgg ccctcgtggc
     5461 gcgccgttat cccaagctcg ggctgcccgc agtttccctc gatgacgcca agggcttgct
     5521 gtttcccttg tgtgaagggc tggttagctt cgaggaagtg cgccgcctcg atttggttgc
     5581 cgagttgctt tcgaggctca cccccgagca acgtcgcgag ctcgatcgcg cggcaccgga
     5641 gagtattcgg ctgccatcgg gacgttccgc gcgaattcac tacgaggcgg gcaagccgcc
     5701 ctggctggag tcgcgcctcc aagacttctt tggcttgaag gaggggccca agctgtgcgc
     5761 gggttcgatc gacgtcacct tgcacttact cgccccgaac ggccgagccg tgcaggtcac
     5821 gagcgacttg ccggggtttt gggaacggca ttatcccacg gtgcgccgcg agctgatgcg
     5881 gcgttatccg cggcatgcgt ggccagagga cgccaagagt gcgccgccga ttcaaccgcg
     5941 tccccgccgc ggggatggct gagttccgct cgcgcctaca gccgcgtctt gaggcgcgcc
     6001 aatagctgga gcgcttccag acccgtcagg cgatcggggt cgaggttacg cagagtctcg
     6061 agtacttcgc gctctttggc ttcgctctgc ttgttggcac cgaataagcc gagttgattc
     6121 tcgggtacgg atttcgggct gcgccgcgcg ccgacgtcaa tccttcgatc cttctccagc
     6181 atggccaaga gggcgcgcgc gcgcgacaac accgactcgg gcaatcccgc aagcttggct
     6241 acggcaacac cgtagctgcg actcgcaggg cccttgccga ctcggtgcag aaacaccacg
     6301 tcatgtccga cctctttggc gctcacgctg tagttgaccg tatgcgtgct cgcttcggcc
     6361 aggccggtga gctcgtgata gtgcgtcgcg aacaacgcgc gacaaccgat ggcttcgtcc
     6421 agatactcgg cgaccgccca agcgatactc aagccatcga acgtgctcgt tccgcgcccg
     6481 atttcatcca gaatcacgag cgatcggcgc gtggcgctgc gcagaatttc cgccgtctcg
     6541 cgcatctcga ccatgaacgt actctcgcca cgcgagaggt tgtcgctggc gcccacacga
     6601 gacaggatac gatcgaccac gccaatgcgc gcggagcgcg cgggaacgaa gctgcccatc
     6661 tgcgcgagca gcgtgattaa cgcaacctgc cgcaagaacg tgctcttgcc ggccatgttg
     6721 gggccagtaa ttagccacag ccgtgcgccc gagctcgcca agtccacgtc gttgggaacg
     6781 aagcgcccga cctccgccaa acgctccacg accgcgtgtc gaccttccct gatcgtgatc
     6841 acatccgact cgtccaccac gggccgtgtg tactcgtggc ggtgcgcgat ctcggccaag
     6901 ccttgagcta cgtcccatcg tgctatgcgc gcagcgagag cgcgcacacg ttcgctatgg
     6961 cgcccggcga gcgcgaccag ctgcttcaac aagtcgagct cacggtcgcg gtggcgctct
     7021 tcggcgtgca ggattcgatc ggagagctca tccagctcgg gggtggtgta acgttccccg
     7081 ctggcgacgg tttgcttgcg tcgaaacccc tccggaactt tcgccgcctg gctgcgcgtc
     7141 acctcgatgt accagccgaa cacccgcgtg tagcggacct tgagcgacgc gactcccgtg
     7201 tcctggcgca gccgagcctc gagctccacc atcttttcgg cgccgcgctt ctgcagatcg
     7261 tcgagctcgt cgagctcgga gtcgaagcca gatcgaaaga ccgaaccctc cttcgccaac
     7321 gccggcggcc gatcgacgag cgcgcgcgca agctcggcct ggagctcggg gacgacgtcc
     7381 acctcgcacg cgcctgaatc accggaaccg ggagcgatga ggcgcaacac ctccttctcg
     7441 cgcgcgtcca gcgcttgcaa gctctcgacc gccgccgccg ccgcggccaa cccgtcgcgg
     7501 agcgcaccca aatccctggg agtggcttcg cctagcgacg cgcgcacggc caagcgctct
     7561 aggtcgccta ccgcactcag aacttcgccg aattgctcgc gcaaggttgg tcgcgcgaga
     7621 aacacctcca ccgcgtcgag ccggcggcga atgcgttcta cgtccagcaa cggagccaac
     7681 aaccgtcgcc gcaacaggcg cgcaccggac ggcgttcgcg ttgcgtcgat gacattcagc
     7741 agcgtcgctt ttcgtcccgc gggggactcg accaactcca ggtgttgttg agcggtggag
     7801 tcgatcgcca ggttctcgct gggctcgaag cgggccacgc gcgtgaccgg caaggtctgc
     7861 ccgggattgc agcgccgagc gaagcgcatt gcgcgcgcca ccgctcgctc gccgctcgca
     7921 ccgacgcgac gcgcctcctc ctgcaggtcg ccgagtactt cgtcgacgct ctccgcctcc
     7981 agcgcgtcgt cctcacgcag cggagcgccc aaggcctgca aagcgcgtag cagcgtctga
     8041 cgctcctcgt cactcgcctt gccgaaacca agcaagattt cccgagggcg cgagcgactg
     8101 agtgcgctca gcagcaacgt agcgtcggcc agaaccgcgg ccgagagctc acccgtcgag
     8161 aggtcgaaca gtgcgagcgc gcttgccccc ggctcgaact cgaccgcggc gaggtaattg
     8221 ttggtgtgtg cgttcaggta gtcggcgtcc gtcagcagtc cgggcgtgat cacccgaacc
     8281 acttcgcgcg ggacgatgcc tttcacctta ctcggatcgg ccatctgctc gcagatggcc
     8341 accttgtgac cgagctccag caacttcgcg atgtagccgt gggcggcgtg atacggcacg
     8401 ccggccatcg gaatctcttc cggcttgcct ttgttgcggc tcgtcaacgt gagatccagc
     8461 aagcgcgacg tcagctgtgc gtcttcgccg aacatctcgt aaaaatcgcc aaggcgaaag
     8521 aatacgatcg cgtccggata ggcgcgcttc gccgccgcgt gctgctgcat caccggcgtc
     8581 ttcgaaccgc tcatctcagt tcaaggtggg gctgatggga gagatagagt ccaagctcat
     8641 gaacacgatc ccgagtgagc tggcatccac cacgctcagc cggtctaggt tcgggaggtg
     8701 cgtaagcgcc gtcggggcga taccgccgga gccgctgggc atgctgatcg ccagcggaac
     8761 gaacgcgccg gtcgtgcgcc agctgaagct catgtcttct tggctctcct cttgtcctcg
     8821 atagattgcg aagcgtgccg tgggcgactc gaagatgcac ttgcgggccg cgtcgcgatc
     8881 cacgtcgaac gggaccacgt tctcgaattg cctttgcgcg ctcgggggct taggcaacac
     8941 gcacggagag ttcgggtcgc tacccacgcg gcatttgtcc gatccattca agcagtagtc
     9001 ggtcggagcc gtgatttcga acactcgaga ttcgaagtac ctcttgcgcg ggttacaatc
     9061 ccggcggcac tcgaaaacac gggtgccgtc ggcgcccctc tcttctcgcg cgatgatcga
     9121 gtgagcgaag cccgccgagc tgcgcaacac ccagctgttg gtcacgcgga tgctgtaccc
     9181 ggttgcgctt gggaaacagg cgtgggc
//
LOCUS       DASTKU010000484         7880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1653803, whole genome shotgun sequence.
ACCESSION   DASTKU010000484 DASTKU010000000
VERSION     DASTKU010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7880
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653803"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VFQ61_16760"
     CDS             <1..635
                     /locus_tag="VFQ61_16760"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9956162.1"
                     /translation="LQIANSAWFATRQEVTDLKGAVRQLGLTTLRNLALGIGAFDAAR
                     SELRHHTKWVEQLQERALRTAKLAGSLVRRGQAANEAFLGGLLCNVGLLALATVSRDR
                     LERSEAEAARSHAPLHQAEAKLWGCTHAEVGAALLGLWGLPFGVVETVLNHHAPERNV
                     RDVLGPPQAVWIAASSVEGEPLDAAYLDKIGATELVRKIQQQGSEVKVGV"
     gene            639..2024
                     /locus_tag="VFQ61_16765"
     CDS             639..2024
                     /locus_tag="VFQ61_16765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9956163.1"
                     /translation="MSGVTHGSTQDVEPAAIPDALPDGVLILTARGEVEWANAAFLKL
                     TERTGQTLRGLPFVALVADEDVLHLVGIDTVFATNATYDNNVIFTTPEGVRRRLIVSS
                     ARSLDGSHIFLTVRATGIVHHELANTARWAATEQDRANELAKVRDALTANNRALQQAR
                     EEVERAYAKLKDEVLARERLENELRLAQKLEAIGQLSAGIAHEINTPMQYIGDNVDFL
                     SRSFAILTEYIADFEQRLEHEHCDALRQDLEQLKRRKRVAFITEQVPRAFADSKSGIA
                     HVSTIVRAMKSFAHSDGDEQTLASLNEILSNTLIVAQNEYKNVASVETRFEDLPPIPC
                     YVGRLNQVFLNLIVNAAHAIEAAARPERGKITVSSKLLEDHVEVSIADTGTGIAPHIR
                     HRVFDQFFTTKPVGKGTGQGLSLARAVVVDAHAGTLTFESEEGVGTVFYVRLPLAGRA
                     SSTPNSSLLPV"
     gene            2199..3434
                     /locus_tag="VFQ61_16770"
     CDS             2199..3434
                     /locus_tag="VFQ61_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9956164.1"
                     /translation="MIAFEKIRDRNIVRIYAATLLIGLGYGISLSVTALHLTARGFDK
                     QDIGSLAAWFAGGLVALSLLMGGLIRRFTAKRVLWLALLGYAASIAIFPTVDTYTGLA
                     AVRAMDGACSVMVWVCSETLLLSRAEPEHKAFTLSLYTITIAIGYVFGPLLAHAIVEA
                     ASMRTAFSCAASLAVLSSLYVVFKLEPDRAPAHESAAATAKDDASRHSYEPFVGLLRK
                     IKNSCFATFCYGYFQATVVLFLPIYLMESKAVPAERTILIPAFFAAGMLLFSNLAGRA
                     GDRFGHLRVMRLLAILGTTMILGFVYLDSYAWMCAAVFIAGASLASISPLSLALQGAQ
                     CDSREYARATAIYNTFYAAGILLGPPLASRIFARAGGPWMLYHLAALWAAFIAFSLWF
                     ASDDPRHRASAAVPSAGSN"
     gene            complement(3446..4063)
                     /locus_tag="VFQ61_16775"
     CDS             complement(3446..4063)
                     /locus_tag="VFQ61_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956165.1"
                     /translation="MARTSALGSLLLRDLLGAVQAGTALAALALVPGCASRSLPNDPE
                     GTGGVRPGDFETGGSTTGGTSETGGSSATGGATSKPEPTPLPESELGCYGTIYGDGFG
                     YHGQCCFTQRCYTPEAGKDCAAPDAVYETDLSLPAGSGTCGCGPEGHKPISGPYAYNP
                     AVETDGTGSCCYVVGSIGCDGRPLIVEGEALLAGLVSRSDWSSWS"
     gene            4216..4707
                     /gene="ybaK"
                     /locus_tag="VFQ61_16780"
     CDS             4216..4707
                     /gene="ybaK"
                     /locus_tag="VFQ61_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HET9956166.1"
                     /translation="MKTNAARILDGLGIPYELAEYQVDPEDLSAVTVARKVGMEPERV
                     FKTLVARGDRHGVCLALVPAHLELDLKALARATDNARAETVPLKDVLPLTGYVRGGVS
                     ALGCKKAYPVICHESMLNFDRVAVSAGVRGTQILLSPQDYVRAVKGRVAPIARPEPES
                     PHA"
     gene            4700..5698
                     /locus_tag="VFQ61_16785"
     CDS             4700..5698
                     /locus_tag="VFQ61_16785"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9956167.1"
                     /translation="MPEPNEDRPGRILSGRYELLEVLGKGGMAVVWRGLLHGADGFRR
                     PVAIKRIDPSCRGFIEVIEMFVEEARVGGLLQHPNIVQVYEFGADEAGERYLVSELVR
                     GPNLGQWLKAYQEDRELPPWELVTQIAVEVLRGLDAAHSHVDARGASAPILHRDVTPG
                     NILIDAAGIVKLADFGLARAMDRGQITRPNIVKGKLSYLAPELLAGGPPSMAADTFSL
                     GVVLWEALAGRRLFQAETDIEVVELVRQCRVPLLSRERTSLPLTLCSCVHQALERDPR
                     RRYASARQMLEQLSEVLRILPQNVDARSLATSLRKVPALIDSDLLQPASPHAGGPP"
     gene            5746..5985
                     /locus_tag="VFQ61_16790"
     CDS             5746..5985
                     /locus_tag="VFQ61_16790"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HET9956168.1"
                     /translation="MNLAELAIGTSAVVTRVGGQGSFRRRLMEHGLLPGTQVELMRIA
                     PLGDPLELLVRGASLSIRRAEALTVEVATGPRGNP"
     gene            5982..>7880
                     /gene="feoB"
                     /locus_tag="VFQ61_16795"
     CDS             5982..>7880
                     /gene="feoB"
                     /locus_tag="VFQ61_16795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HET9956169.1"
                     /translation="MTASFSTPGEPEPALSPETVIEAPGRVEGATRLVVLVGNPNVGK
                     TTLFNDLTGQNARIGNYPGVTVERRSGIVKHAHDDPFELVDVPGTYSLAARSAEEQIA
                     IASVLGLGEHPRPELCLVVVDAGQLGRNLYLVLQLLELGVPTVVAVNMMDEVSDNPPN
                     LALLERELGVPCVPTDASRKAGIPELLRSVVATLKQTRQSPPHINYPRELIRDADRVA
                     EELPPELKGNVERTRAAAMWALSSIDDDDELQGIPAALRERCADVRRAAQGRDVDREI
                     IQSRYDFIDQLLPRLYQDPKAHPPKRRVSERVDNVLLHPLLGFLAFIAIMLVVFQALF
                     SWSEPMVGWIEDAVTLASRFVKSTLPESTLRDLLTEGVLGGVGNVIVFLPQILLLFLF
                     IGFLEDSGYMARVAYLMDRIMKALGLHGRAFVPMLSGFACAVPAILATRTMERQRDRL
                     LTMLVVPLMTCSARLPVYSLLIATLFPADARVGFVSLQSTLLVAMYVFSMAMTLVVAG
                     ILGRTVVRARRVPLLLELPPYRMPRLAGTLRQMRERASVFLKEAGTVILGCTIVLWAL
                     LSFPRDSSPDEQVTQPAAVVLQNTTPPAANAAPSAEAEQAAIEKSAIEKSYGGQLGKA
                     IEPLLKPLG"
BASE COUNT         1377 a         2549 c         2478 g         1476 t
ORIGIN      
        1 tgttgcagat cgccaactcg gcctggttcg cgacgcgaca ggaggtcacg gacttgaagg
       61 gtgccgtacg ccaactaggg ctcactacgc tgcgcaacct ggcgctcggc atcggcgcct
      121 tcgacgcggc ccgcagtgaa ctgcgacacc acaccaagtg ggtcgagcaa ctgcaagaac
      181 gcgcgctacg caccgccaag cttgcggggt cgctggtgcg ccggggccaa gccgccaacg
      241 aagccttcct cggcggcctg ttgtgcaacg tgggcctgtt ggcgctggca acggtgtcgc
      301 gtgaccgcct cgagcgttcg gaggccgaag ccgcccgatc gcacgccccc cttcaccaag
      361 cggaggccaa gctgtggggc tgcacgcacg cggaagtggg cgcggccttg ctcggattgt
      421 gggggctgcc gttcggcgtg gtggagacgg tgctcaatca ccacgccccg gagcgcaatg
      481 tgcgagacgt cctcggccca ccgcaagcag tttggatcgc ggcttcgtcg gtcgaaggtg
      541 agccgctcga tgcggcttac ttggacaaaa tcggcgccac ggagctggtc cgaaagattc
      601 agcagcaggg ttcagaagtg aaagtgggag tataagcaat gagtggtgta acccacggtt
      661 cgacgcaaga cgtcgagcct gcagccatcc cggatgccct ccccgatggc gtcctgattc
      721 tcacggcgcg cggcgaagtg gagtgggcca acgccgcgtt cctcaagctc accgaacgca
      781 ccgggcaaac gctgcgcgga ctgccgttcg tggccctggt ggcagacgag gacgtgcttc
      841 acttggtcgg catcgacacg gtattcgcga ccaacgccac ctacgacaac aacgtgatct
      901 tcaccacacc cgagggcgtg cggcgacgct tgatcgtgtc ctcggcgcgc tcgctcgacg
      961 gcagccacat cttccttacc gtgcgtgcca cgggcatcgt ccaccacgag ctcgcgaaca
     1021 cggcccgctg ggccgccacc gagcaggatc gcgccaacga actggccaaa gttcgcgacg
     1081 ccctaaccgc caacaaccgc gccttgcagc aggcccgtga agaagtcgaa cgcgcctatg
     1141 cgaagctgaa ggacgaggtg ctggcacgcg agcgcttgga gaacgagcta agactggcgc
     1201 agaagctcga ggccatcggc cagctctcgg caggcatcgc acacgagatc aatacgccga
     1261 tgcagtacat cggggacaac gtcgatttct tgtctcgttc gtttgcgatc ctgaccgagt
     1321 acatcgccga tttcgagcag cggctcgagc acgaacactg cgacgcgctc cgccaagacc
     1381 tggagcaact caaacgccgc aagcgcgtcg cgttcatcac ggaacaggtg ccccgcgcct
     1441 ttgcggactc taaatctggg atcgcgcatg tttcgaccat cgtccgggcc atgaagtcgt
     1501 tcgcgcacag cgacggagac gagcagaccc tcgcgagcct gaacgagatc ttgagcaata
     1561 cgctgatcgt cgcccagaac gagtacaaga acgtcgccag cgtagagaca cgcttcgagg
     1621 atctaccgcc gataccttgc tacgttggac ggctgaatca ggtctttttg aacctgatcg
     1681 tgaacgccgc gcacgcgatc gaggccgccg ctcgccccga gcgcggcaag atcaccgtct
     1741 catcgaagct gttggaggac cacgtcgagg tgagcatcgc ggacacgggc acgggcatcg
     1801 ctccccacat ccggcatcgc gtcttcgacc agttcttcac caccaagccg gtcggcaagg
     1861 gcacggggca gggcctctcg ttggcgcgag cagtagtcgt ggacgctcac gcgggcacgc
     1921 tcaccttcga gagcgaagag ggcgtgggga ccgtcttcta cgtaagattg ccgctcgctg
     1981 gtagggcgag cagcaccccg aattcatctt tgttgccggt gtagccgacc tttttgccgc
     2041 aagagccggg ctcttcggct ccgaagcgga ttcaacggcc cggaagtgct ggggccacgc
     2101 gctctcggag cgggtgtgcc cgggggaacc ggacacccct gggatggctt attcgcccgc
     2161 ttcttgatac tcagaggtca cggccaggcc ctcacctcat gattgcattc gagaagatcc
     2221 gggaccgaaa catcgtccgc atctacgccg ccacgttgtt gattgggctc ggttacggaa
     2281 tctcactctc tgtaaccgct ctccacctga ccgcgcgggg cttcgacaaa caggacattg
     2341 gtagcctggc ggcttggttt gcgggagggc tggttgcgct atcgctactg atgggcggcc
     2401 tcattcggcg cttcacggcc aagagagtgt tgtggctcgc actgctcggc tacgcagcga
     2461 gcatcgcgat ttttccgacg gtcgacacct atacgggtct cgccgcggtg cgtgcaatgg
     2521 acggggcctg ttcggtgatg gtgtgggttt gctcggaaac gctgctactc tcgcgcgcag
     2581 agcccgagca caaagcgttc acgctcagcc tttacacgat cacgattgcc atcggttacg
     2641 tgtttggacc cttgctcgcg cacgcgatcg tggaggcagc gtcgatgcgc accgcgtttt
     2701 cgtgtgcggc gagcctggcc gtgttgtctt cgctctacgt ggtattcaag ctcgagcccg
     2761 atcgtgcgcc ggcccacgaa agcgccgccg caacggctaa ggacgacgct tctcgccact
     2821 cgtacgaacc cttcgtgggc ttgctcagga agatcaagaa ctcgtgcttc gcgacgttct
     2881 gctacggcta ctttcaagcg accgtagtgc tctttctccc catctatctg atggagagca
     2941 aggccgtgcc cgcagagcgc accatcttga ttccagcgtt ctttgccgcg gggatgctgc
     3001 tgttctccaa cctcgcgggg cgcgcaggcg atcgcttcgg ccacttgcgt gtgatgcgcc
     3061 tgctggcgat cctcggaacg acgatgatcc tcggcttcgt gtacctcgac tcctacgctt
     3121 ggatgtgcgc cgccgtattc atcgcgggcg cgagcctggc gtccatctct ccgctcagct
     3181 tggctttgca gggagcccag tgtgatagcc gtgagtacgc gcgcgcgacc gcgatctaca
     3241 acacgtttta cgccgcggga atcctgctcg ggccgccgct cgcgagtcgt atcttcgctc
     3301 gagctggcgg cccctggatg ctctaccacc tggccgcgct ctgggcggca ttcatcgcct
     3361 tctcactgtg gttcgcgagt gacgatccga ggcaccgcgc gagcgccgcg gtgccctccg
     3421 cgggctcgaa ctaaccccgc tcgagctagc tccagctgga ccagtcggac ctcgaaacca
     3481 gccctgccaa tagcgcctcg ccctccacga tcaagggacg cccgtcgcaa ccgatcgaac
     3541 cgaccacgta gcagcagctt ccggttccat cggtctcgac cgcaggattg taggcgtaag
     3601 gcccggagat gggcttgtgc ccctctggtc cgcaaccgca cgtgccggat cccgcgggca
     3661 atgacaggtc agtctcgtag accgcgtccg gtgccgcgca gtccttcccg gcctcgggtg
     3721 tgtaacagcg ttgggtgaaa cagcattggc cgtggtagcc gaacccgtct ccgtaaatcg
     3781 tgccgtagca gcccagttca ctttcgggta aaggcgtcgg ctcgggtttg gaggtggctc
     3841 cgcccgtcgc actgcttcca cccgtttcgc tggtcccgcc tgtcgtgctg ccacccgtct
     3901 cgaagtcacc gggacgtacg ccgccggttc cctccggatc gttgggaagc gaccggctcg
     3961 cgcagccggg aaccaaagcc aacgccgcca aggcggtgcc cgcctgcacg gcgccgagca
     4021 ggtcacgcaa gagtagagat ccgagagctg atgtcctggc cattcgcggc cgtatcttca
     4081 atttgcgtgc caccggtttt gcaggcaaat tgctgaggct tggcacgcag agtgagccaa
     4141 gttgtgccgg cgagcccgcc gtacctgcta caccgccctg ctaaaaccgc ctaaaccccg
     4201 cccacacgct ccgcgatgaa aaccaacgca gcacgcatct tggacggcct cggtatcccc
     4261 tacgagctcg ctgaatacca agtcgatccc gaagatctga gcgccgtcac ggtagcgcgc
     4321 aaagtcggca tggaacccga gcgcgtattc aagaccttgg tggctcgggg cgatcgacac
     4381 ggcgtttgtt tggcactggt acccgcacac ctggagctcg atttgaaggc gctggcccga
     4441 gctacggaca acgcccgagc cgaaaccgtt ccgctcaagg acgtgctgcc gctgacgggt
     4501 tacgtccggg gcggggtgtc ggcgctcggg tgcaagaagg cctaccctgt catttgccat
     4561 gagagcatgt tgaacttcga tcgcgtcgcc gtatccgctg gggtacgcgg aacacagatc
     4621 ctgctctcgc cgcaggacta cgtgcgggcc gtgaaaggcc gcgttgcccc gatcgcccgc
     4681 ccggagccgg aatctcccca tgcctgaacc caacgaagat cgccccggtc gcattctttc
     4741 cggtcgctat gagctcctcg aggtgcttgg caagggcggt atggccgtcg tctggcgcgg
     4801 cctgttgcat ggcgccgatg gctttcgccg ccccgtcgcg atcaaacgta tcgacccgag
     4861 ctgtcgaggc ttcatcgaag tgatcgagat gttcgtggaa gaggcccgcg tcggaggcct
     4921 cttgcagcac ccaaacatcg tacaagttta cgagtttggc gcagacgagg ccggtgaacg
     4981 ctacttggtg agcgagctcg tgcgcgggcc gaacctcggg caatggctga aggcgtatca
     5041 agaagatcgt gagctgccgc cctgggagct cgtgacacag atcgccgtgg aagtcttgcg
     5101 cggcttggat gccgcccact cgcacgtgga tgcacggggc gcgagcgcgc cgatcctgca
     5161 ccgcgacgtc accccgggca atatcttgat cgacgccgcc ggcatcgtga agttggctga
     5221 cttcgggctc gctcgggcca tggatcgcgg ccagatcacg cgtcccaata tcgtcaaagg
     5281 caagctcagc tacttggccc ccgagctgct cgccggaggt ccccccagca tggccgccga
     5341 caccttcagc ttgggggtgg tgctctggga agcgctcgcc ggtcgccgat tgtttcaggc
     5401 tgaaacagac atcgaggtcg tggagctcgt tcggcagtgt cgcgttcctc tcctgagtcg
     5461 cgaacgcacg tctctaccgc tcacgctgtg ctcgtgtgtc catcaggcgt tggagcgtga
     5521 tccgcgacgg cgctacgcct ccgcccgaca aatgttggag caactctcgg aggttttacg
     5581 catcctcccc caaaatgtgg atgctcggtc cctagccacg agcctgcgga aggtgcctgc
     5641 gctaatcgat tcggacctgc ttcaaccggc atctcctcat gcgggaggcc ctccatgaat
     5701 ttgaaagtcg ttttcaagat tgacttctca gtgtagaatc ggcccatgaa ccttgcagag
     5761 ttagcgatcg gaacgagcgc ggtggtcacg cgcgtgggtg gccaaggttc ttttcgacgg
     5821 cgcctgatgg aacatgggct gctgccgggc acgcaggtcg agctgatgcg gatcgcgccc
     5881 ctcggagacc ctttggagct gctcgtccgc ggcgcgagcc tgtcgatccg ccgagccgaa
     5941 gcgttgaccg tggaagttgc gacggggccg cggggcaacc cttgactgca agcttcagca
     6001 ctcccgggga gcccgaacct gcactgagcc ccgaaacggt gatcgaagct ccgggtcgcg
     6061 tggaaggcgc cacgcggctc gtggtgctcg tcggaaaccc gaacgtcggt aagactaccc
     6121 tcttcaacga tctcaccgga cagaacgcgc gcatcggcaa ttaccccggg gtcacggtcg
     6181 aacggcgctc cggcatcgtc aaacacgcgc acgacgatcc tttcgagctc gtcgatgtgc
     6241 ccggcaccta ctcgttggcg gcccgctctg ccgaggaaca aatcgcgatc gcgtcggtgc
     6301 tcgggctcgg cgagcacccg cgtccggaac tttgtctggt ggtggtagac gccggtcagc
     6361 tcgggcgcaa tctgtacctg gttctgcagc tgttggagct cggcgttccg accgtcgtgg
     6421 ccgtaaacat gatggacgag gtctccgaca acccgcccaa tctggcgttg ctcgagcgcg
     6481 aactcggcgt cccgtgtgtg cccaccgacg caagccgaaa ggccggcatc cccgagctac
     6541 tgcgcagcgt ggtcgccacc ttgaagcaga cccgccaaag cccgcctcac atcaattatc
     6601 cgcgggagtt gatccgcgac gcggatcgag tggcggagga gctgccgccg gagctcaagg
     6661 gcaacgtgga gcgcacgcgc gcggccgcca tgtgggccct gtcgagcatc gatgacgatg
     6721 acgaactcca aggcatcccc gcggcgctgc gcgagcggtg tgccgacgtg cggcgagccg
     6781 cgcaaggccg cgacgtggat cgcgaaatca tccagagtcg gtacgacttc atcgatcagc
     6841 tcctgccccg cctgtatcag gacccgaaag cgcatccgcc gaagcgtcgc gtgtcggagc
     6901 gcgtggataa cgttttattg cacccgctgc tcgggttttt ggcgttcatt gccatcatgt
     6961 tggtggtgtt tcaggcgctg ttctcgtggt ccgagccgat ggtgggctgg atcgaagacg
     7021 cggtaaccct ggcatcccgc ttcgtgaaga gcacgttgcc cgaaagcacg ctgcgcgacc
     7081 tcctgaccga gggcgtactt ggtggcgtcg gcaatgtcat cgtgttcctg ccgcagatct
     7141 tgctgctgtt cttgttcatc ggctttctcg aggacagcgg ctacatggcc cgcgttgcct
     7201 atttgatgga tcgcatcatg aaggctctcg gcctgcatgg gcgggccttc gtgcccatgc
     7261 tctccggttt cgcgtgcgca gtgccggcga ttctagcgac ccgaaccatg gagcggcagc
     7321 gggaccggct gctgacgatg ttggtggttc cgctcatgac gtgttcggcg cgattgccgg
     7381 tctacagctt gttgatcgcg accttgttcc ccgcggacgc tcgcgtaggc ttcgtctcgc
     7441 tgcagagcac attgctcgtc gccatgtacg tgttctcgat ggcgatgacc ctcgtcgtgg
     7501 cggggattct cgggcgcacg gtcgtcaggg cgcgccgcgt cccgttgctg ctcgaactcc
     7561 ccccgtaccg aatgccccgc ctggcgggaa cgctccggca gatgcgagag cgcgcttcag
     7621 tcttcctcaa agaggcggga acggtcattc tgggctgcac gatcgtgctg tgggcgctgt
     7681 tgtccttccc acgcgacagc agccccgatg agcaagttac gcagccagcg gcggtagttc
     7741 tacagaacac gactccgccc gcggccaacg ccgcaccctc cgccgaggcg gagcaggctg
     7801 ccatcgagaa gagtgccatc gagaagagct acggcgggca actcggcaag gccatcgaac
     7861 ctctgctgaa gccgctcggt
//
LOCUS       DASTKU010000485         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_92801,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000485 DASTKU010000000
VERSION     DASTKU010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92801"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1117)
                     /locus_tag="VFQ61_16800"
     CDS             complement(35..1117)
                     /locus_tag="VFQ61_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956170.1"
                     /translation="MKISALLAMATACLLTSGFAFATHKAWLMKNSGADCQFLSATAG
                     DTVTTGGALKNSTAGNRTALCPQTLASRWGSNGTPVFDARTGVDARHAAVYLNHQTAS
                     SAASCYAVMTASTGALYYSATVSDSGAAGVGRITLANQIGDTGAWAAGLGNTIEANED
                     LYAVGLAYVCTLPPNTSVLGTKVKLCQLSTDCHESGSDAEFDHVGGTSTLRQNVQTNG
                     IECSATQNPSQIVKSENGIKNNGPAANTVFCPIVPPADDTAEVTRDMWFTSVYYSGGS
                     DASNCVSSGTCPSCNLTWTDHAGVVSNGAVIAKDTSATFKLSQNGPVRVGTEVAVGVL
                     CTLPAATTLEGITAQTTITGVQQGGM"
     gene            complement(1155..1739)
                     /locus_tag="VFQ61_16805"
     CDS             complement(1155..1739)
                     /locus_tag="VFQ61_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956171.1"
                     /translation="MVRRTRTTILVFVTGVAVTTAAGAQYVYTRGLNYVDTAGSRCVV
                     GAANRQADVGHYTDGVAVSSSVSTSRTVYCPINRRSLNYYEQGSDTVNAKSVTVNNVN
                     FRVYDASSTQSLSCKLFFKGVGGSVSYSATRYACSSFLGCSTDPGSSFTGMNVMAVSG
                     QIINTNTVEWGLMCSLPSSSQIYAYEAFIKPNAN"
     gene            complement(1810..2583)
                     /locus_tag="VFQ61_16810"
     CDS             complement(1810..2583)
                     /locus_tag="VFQ61_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956172.1"
                     /translation="MGVVTGRSGGRGAVSSELRSQPRANVLRSKPLAKLLGGLAIVSA
                     LAGAYWLGAQRSKYSDPAETSAKGALPDARTAPPIILTVSPAQPVARDDARVAPAAVE
                     PAPAELPSGSGDPRPQLSPLEHRDRVLTALRASGRDTRLTSFGQTVVKGWASKLQQLA
                     VGGKVDPLECYAKGCAVTIEYPSQSAAEDGNEVVTRSGEFNGWQAGKMRSGYITKSNG
                     AVEVTWILDSPPADSPVLPDKLPADNFDELKEMLARSPK"
BASE COUNT          466 a          750 c          848 g          534 t
ORIGIN      
        1 cgctggggaa ccggtttaaa ggaggcggtt tcagctacat gccgccttgt tgtacgccgg
       61 tgatcgtagt ctgtgcggtt atgccttcca gggttgtggc ggctggcagg gtgcagagca
      121 cgcccacggc gacttcggtg ccaactcgaa ctgggccgtt ctggctgagc ttgaaggtcg
      181 cgctcgtgtc tttggctatg acagcaccgt tcgagacaac gccagcgtgg tcggtccacg
      241 tgaggttgca actgggacac gtgccggagg aaacgcagtt cgaagcgtct gagccgcccg
      301 agtagtacac cgacgtgaac cacatgtcgc gcgtgacttc ggcggtgtcg tccgcagggg
      361 ggacgatcgg gcaaaaaacg gtattcgcag cggggccgtt gtttttgatg ccgttttcgc
      421 tcttcacgat ttgtgaaggg ttctgcgtgg ccgagcactc gatgccatta gtctggacgt
      481 tctgtctcag agtgctggtc ccgcccacat ggtcgaattc ggcgtcgctg ccggactcgt
      541 ggcagtccgt cgagagctgg cagagcttta ccttggtgcc aagcacggac gtgttggggg
      601 gaagggtgca aacgtacgcg agccccaccg cgtaaagatc ttcattggct tcgatggtgt
      661 tgcccaaacc tgccgcccaa gcgccggtgt caccgatctg attcgccaga gtgattcggc
      721 cgacgccggc cgctcccgag tcggagacgg tggcgctgta gtagagagcg ccagtgctcg
      781 ccgtcatgac ggcgtagcag ctagcggcac tcgacgcagt ctggtgattc aggtagacgg
      841 ccgcgtggcg agcgtcgacg ccggttctcg cgtcgaagac cggagtgccg ttcgagcccc
      901 atcggctcgc aagggtttga gggcagaggg ccgttcgatt gccggcggtt gagttcttga
      961 gcgcgccgcc ggtcgtgacg gtgtctcccg cggtcgcgct caagaactgg cagtcagctc
     1021 ccgagttctt catgagccac gccttgtggg tcgcgaacgc gaagccgctc gtgagaaggc
     1081 atgccgtagc catggccaaa agtgctgaaa tcttcatcag atctgctcct tggtggggaa
     1141 ttgggagctc gcgctcagtt ggcgttgggc ttgatgaaag cctcgtacgc gtaaatctgt
     1201 gagctagagg ggagcgagca catgaggccc cactccaccg tgttggtgtt gatgatttga
     1261 ccgctcacgg ccatgacatt catcccggtg aaggagctgc caggatcggt gctgcaaccc
     1321 aagaagctcg aacacgcgta ccgggttgcg ctgtaagaga ccgaaccacc cacacccttg
     1381 aagaacagct tgcaactgag cgactgcgta gaagaagcgt catacacgcg aaagtttacg
     1441 ttgttgaccg ttacgctctt tgcgttgacg gtgtcggagc cctgctcgta gtagttgagg
     1501 ctgcgccgat tgatggggca gtacacggtg cggctcgtcg acaccgacga cgacacggcg
     1561 accccgtcgg tgtaatgccc gacgtccgct tggcgattgg cggctccgac cacgcagcgg
     1621 gaaccggcag tatccacgta gttgagccca cgtgtgtaga cgtattgcgc gccggccgcg
     1681 gtcgttacag cgactccggt aacgaatacc aaaattgttg tccgagttct tcgtaccatt
     1741 cttcaacctc cactgtgttg cgatgcaagc tcagtccgct ggcgcgaacg cgcgagtcgt
     1801 tggaacagtt cacttcggag aacgcgcgag catttccttg agctcgtcga agttgtcggc
     1861 aggtaacttg tcgggaagta cggggctgtc cgccggcggc gaatcgagga tccacgtgac
     1921 ttcgaccgcg ccgttgctct tcgtgatgta gccgctgcgc attttgcccg cctgccagcc
     1981 attgaactca cccgagcgag tgacgacctc gttgccgtct tccgcggcgg attgggaagg
     2041 gtactcgatc gttacggcgc agcctttcgc gtagcactcg agaggatcga cttttccacc
     2101 gactgccaat tgctgcagct tgctcgccca gcccttgacg accgtctgcc caaaagaagt
     2161 cagtcgagtg tcgcgaccag atgcgcggag cgcagtcagc actcgatctc ggtgttcgag
     2221 cggcgaaagt tgcgggcgag gatcaccgga tccggagggg agctcggccg gcgcgggttc
     2281 gactgcagcg ggtgcaacgc gagcgtcgtc tcgcgcgact ggttgcgctg ggctcaccgt
     2341 caatatgatg ggcggcgcgg tgcgtgcatc tggaagcgca cctttcgcag aggtttcggc
     2401 tggatcgctg tacttgctgc gttgcgcacc aagccagtac gcccccgcga gagcgctgac
     2461 gatggctagc ccgccgagta gctttgcgag cggcttgctg cggagaacgt tggcccgtgg
     2521 ctgacttcgg agttccgacg aaacagcacc gcgcccaccg gaccttcccg tgacgacacc
     2581 catcagcggg ctatatca
//
LOCUS       DASTKU010000486         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1207785, whole genome shotgun sequence.
ACCESSION   DASTKU010000486 DASTKU010000000
VERSION     DASTKU010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207785"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VFQ61_16815"
     CDS             <1..1248
                     /locus_tag="VFQ61_16815"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9956173.1"
                     /translation="LAYDLFISAGYPAPRCNFAHVTINGRDAGIYTQVEAIDESLLRE
                     RFGSANGPLYRGELADFDPRTEPLLFNSTQNGSRTALRKLIDALDAPDAELVEHLEQV
                     LDLNAFRDFWALETLIGHWDGYTNNADNFYAYFDPHQKRFVFLPWGADQAFVGTRPWL
                     SGYEPTVYYKGKIANRLYQIPKQRALFRKRLTQHNDRIWSVPDLLARIDALAALTGSG
                     DAQALEELREYVRTRGESLRAALAEPAPEVPRFAPFEPTPLADSCAGQLTELSGSFSV
                     RYGDNPYAAVPGEGQLRITSYVDGQPYTGEFFGAFGPNPILTDHTSLLVATDLPSGMR
                     IGYSLLLPNELYVPGVHPMHAWETLGIAVVIGPGTLNLTDFGYIGDGQITLDRVSHEV
                     GGTVSGSFSGKLYPFGCLHASAQ"
     gene            complement(1333..2034)
                     /locus_tag="VFQ61_16820"
     CDS             complement(1333..2034)
                     /locus_tag="VFQ61_16820"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HET9956174.1"
                     /translation="MNRLRIDIWSDIACPWCYVGKRRLEAALAGFEHQDSVDVVWRSF
                     ELDPDAPAEHDASQSYAERLARKYRRTVGEAETMIARMTEVGRAEGLELRFDRVKRVN
                     TFEAHRLIHLAHEQGKQDVVKERLLRAYMTEGEVISERDVLVRLAGDCGLESEAVRTA
                     LRGEAFAQDVRADEREARELGITGVPFFVFAERYAASGAQPPEVLANALEQAWRDVNA
                     ATSFAEGAACGPDGC"
     gene            complement(2211..>3639)
                     /locus_tag="VFQ61_16825"
     CDS             complement(2211..>3639)
                     /locus_tag="VFQ61_16825"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET9956175.1"
                     /translation="WSQRFEPDCSFPLDAICLHAMASRLYPTLPRQSLRALAGYLGHS
                     LHLERRSLGHVEASAFVWRRMCQELAGRGIVSWEELKDWLAERAPPKPRSKKPQYPID
                     SARYRMLPDQPGVYRFLRSNSDVLYVGKATSLKKRTASHFRARPGTPQSPEMLTQVAD
                     IAVTVTASALEACLLENELIKQLRPPYNVQLTVNDHPLWFSRPDFQAARPERGAEFVL
                     GPLSSELSLRPLAALISLLEGVAPSLALRAQVVGVSALYPPDEVVFGEGWSAFVQRHA
                     AELETESLTPRRRALRVARKLTLAGAGKKEEEEDEAEATSEPGDRRWDGPRVVRHIER
                     AVSQAYRSYRRARWLDLLQNSEVIYREPGKETQPRLLLVKRGEILEARDAEIPHETIC
                     NSGPCLPTPELRAADLPSPQSSRSLPSPFDRAKYDRLRILTTELKRILRDGGEVSVHV
                     SRGSAALGISRRLPERWLSGALKLV"
BASE COUNT          687 a         1207 c         1069 g          676 t
ORIGIN      
        1 ctggcgtacg acctgttcat cagcgcgggt tatccagcac cccgctgcaa tttcgcgcac
       61 gtgacgatca acggccgcga tgcgggaatc tacacgcaag tagaggcgat cgacgagagc
      121 ctgctgcgag agcgattcgg tagcgccaat ggaccactct accgcggtga attggccgat
      181 ttcgaccctc gcaccgagcc cctgctcttc aactccaccc agaatggctc tcggacggcg
      241 ttgcgcaagc tcatagacgc cctcgacgcg ccggacgctg agctcgtcga acacctggag
      301 caggtactcg acttgaacgc cttccgcgac ttctgggcgc tcgaaaccct gatcggacat
      361 tgggatggct acacgaacaa cgccgacaat ttctacgcct atttcgaccc gcaccagaag
      421 cgcttcgtgt tcctaccttg gggagccgat caggccttcg taggcacacg tccgtggctc
      481 agcggttatg agcccacggt ctactacaag ggcaaaatcg ccaatcggct gtatcaaatc
      541 cccaaacaac gtgcactctt tcgaaagcgc ctgacccagc acaacgatcg aatctggagt
      601 gttccggatc tgctcgctcg catcgatgcg ttggccgcgc taacgggctc gggggatgcg
      661 caagcactcg aggagctgcg tgagtacgtc cgcactcgcg gtgagtcact gcgcgccgcg
      721 ctcgcggagc cggcgccgga ggtaccccgg tttgctccct tcgagccgac accgctcgca
      781 gactcgtgcg cgggccagtt gaccgagctg tccggctcgt tctccgttcg atacggagac
      841 aatccctatg ctgccgtacc gggtgaaggc caacttcgga tcacgtcgta tgtcgatggg
      901 cagccttaca cgggtgagtt cttcggggcc ttcgggccca atcccattct cactgaccat
      961 acttcgctgc tagtagcgac ggatcttccg agcggcatgc gcatcggata ttcgctcttg
     1021 ttacccaacg aactctatgt ccccggcgta caccccatgc acgcttggga gaccctgggc
     1081 atcgccgtcg tcattggccc cgggacgctc aatctgaccg acttcggtta cattggtgac
     1141 gggcaaatca ccttggatcg agtcagccat gaagtgggag gcacggtatc cggcagcttc
     1201 agcggtaagc tctatccgtt cggttgcctg cacgccagcg cccagtagcg cggaccggcg
     1261 cggctttgct gacaatgccg tctcgccgct tcacacccgc ttcgtgttcg aacgtgggcc
     1321 tcgctcgagg actcaacaac cgtcagggcc gcacgccgct ccctccgcga aactggtggc
     1381 ggcgttgacg tctcgccagg cttgctcgag agcatttgcc aacacttcgg ggggctgcgc
     1441 gccggatgcc gcatagcgtt cggcaaagac gaaaaaggga acgccagtaa ttcccagctc
     1501 ccgagcctcg cgttcgtctg ctctgacgtc ctgagcaaat gcttcacccc gcagtgccgt
     1561 ccgaaccgct tctgattcca gaccgcaatc tccggctagc cgcaccagga cgtctcgctc
     1621 tgagatcacc tcaccctcgg tcatatacgc gcgcagcaga cgttctttga cgacatcctg
     1681 ctttccttgc tcgtgggcga gatggatcaa ccgatgtgcc tcgaacgtgt tcactcgttt
     1741 gacgcggtcg aagcgtagct ccaagccctc cgcccgccct acttccgtca tgcgcgcgat
     1801 catcgtctcg gcctcgccca cggtccggcg atactttcgc gccaggcgct ccgcatagga
     1861 ctggctcgcg tcatgctccg ctggcgcatc cggatcgagc tcgaaagagc gccagacaac
     1921 gtccacggag tcttggtgct cgaagccagc caaagctgct tctaggcggc gcttacccac
     1981 gtagcaccag ggacaggcaa tatccgacca aatgtcgatg cgcagccgat tcatagcgca
     2041 agcataaggc cacccgaggc gattggcagg gggacacgag caagcctgat gtccgaaccg
     2101 gaccatcgag gttgtcgggg ccggccacga gcccccgcag agccgccacg gctcgccaac
     2161 tcgcgccaca agcttcgcgc ctcgtcgagc caagacttgg cccggaaaac tcaaacgagc
     2221 ttgagagctc ccgacagcca ccgctccggc agccgacgac tgattcccag agctgcgctg
     2281 cctctcgata cgtgcacgct cacctctccc ccgtcccgaa ggatccgctt cagctcggtc
     2341 gtgaggattc gcaaacggtc gtacttcgcg cggtcgaaag gtgatggcag ggagcgcgaa
     2401 gattggggcg atggcaaatc cgctgctcgt agttcggggg tcgggaggca cgggcccgag
     2461 ttgcagattg tctcgtgcgg tatctcggcg tctcgcgcct ccaatatttc tccgcgcttg
     2521 accaacaaca gacgaggctg agtctccttg cccggctccc gataaattac ctcgctgttc
     2581 tgcagtaggt cgagccagcg cgcacgccgg taacttcggt aagcttgcga caccgctcgc
     2641 tcgatgtgac gaaccacgcg ggggccgtcc cagcggcggt cccccggctc ggaggtggct
     2701 tccgcctcgt cctcttcctc ctctttctta ccggcgcccg ctagggtcag ctttctggcc
     2761 acgcgcagcg cacgccgacg cggggtgagc gattctgtct cgagctctgc cgcgtgacgc
     2821 tggacgaacg ccgaccaacc ctctccaaag accacctcat ccggaggata cagcgcggac
     2881 acgccgacga cctgggcgcg cagcgcaagg ctcggggcaa ccccttcgag tagtgaaatc
     2941 agagccgcca gcgggcgcag cgagagctcc gaagacaacg ggcccagcac gaactcggca
     3001 ccgcgttcgg ggcgggcggc ttggaagtct ggtcggctaa accacagggg gtgatcgttg
     3061 acggtcagct gcacgttgta aggcggacga agctgtttga tgagctcgtt ctccaataaa
     3121 cacgcctcca aggcgctggc cgtgacggtc accgcgatgt ccgcgacttg agtcagcatc
     3181 tcgggtgatt gcggggttcc cggtcgagcc cggaagtggc tggccgtgcg cttcttcaaa
     3241 ctcgtggcct tgccgacgta aagcacgtcg gagttgctgc gcaagaaccg atataccccc
     3301 ggttggtccg gcagcatgcg gtaacgcgcg ctgtcgattg gatattgcgg cttcttgctg
     3361 cgcggcttgg gcggagcacg ctccgccagc caatccttca gctcctccca gctcacgatt
     3421 ccacgcccag ctagctcttg gcacatgcgt cgccaaacga atgccgaggc ctcgacgtgc
     3481 cccagcgaac gacgctccaa gtgcagactg tgacccagat aaccagccag tgcgcgcaag
     3541 gattgtcgag gcagagtcgg atacaaacga ctcgccatgg catgcagaca gatcgcatcg
     3601 agcgggaagg agcagtccgg ctcgaagcgt tgcgaccaa
//
LOCUS       DASTKU010000487         6938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_92851,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000487 DASTKU010000000
VERSION     DASTKU010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92851"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VFQ61_16830"
     CDS             <1..911
                     /locus_tag="VFQ61_16830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9956176.1"
                     /translation="AKNIDFRAACGCSDWAVLGDLGLMSNALMNLCLNSADAITERGT
                     IVIETKLIELAEAAALAFGVPPGSYLELAVRDDGYGMAPEVLARAFEPFFSTKDSKRR
                     SGLGLPMVYGAVQQHRGGLKIESEPGRGTRVQIVLPAYQRLSAPAPEKAKKTPVVSSS
                     RPLALFVDDEPLLRKAGKRMLSGLGYEVVLASNGHDAVQKFNEHRHRIGVVILDVAMP
                     VMGGAECCEQLRKVDPDLPVVFASGFPKGHDVQSLTSFPKTRYVHKPYELDDISNALA
                     ELAEAFRPSVRLACQANLVRIQDARA"
     gene            1984..3564
                     /locus_tag="VFQ61_16835"
     CDS             1984..3564
                     /locus_tag="VFQ61_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956177.1"
                     /translation="MSPCKRSWTRRNWAFSLVSALSCATALDARAQAAPPAPAPTEPA
                     RPAPAQPAPDAPPAANPVAPAPDAKPTPLPGAPAPAGLGSAPADANTEVAGSNAATEP
                     ASAEAGTPGATAGAAEAEADADAAAAAFMASQEVDAGATANSDYKINIYGFADFTYSV
                     TANKNEFRVTKPSFMVGNFNVYLGGELGDGWRTLTEVRYLYLPHGTPPASQSFAVAPE
                     RIDTTIQDHADVRGTIRWGGISIQRVYVEKTIKPWLTVRGGQFLTPYGIWNVDHGSPV
                     IIGVRRPYIIGETVFPAQQTGLEVFGSQLFGDLELGYHLTLSNGRGPVDAYMDLDGNK
                     AVGARAWGRYRSELGIVTLGFSGYRGKYTNRTQRFNFGADNSLQVTQPLVEQYDELSL
                     AVDLKWERKGFLFQTEAMLNDVSYNERVRPQWAFVTSGPPGFKPDYRRRGFYTLVGYR
                     FDFFGIMPWVQGEYFNSGLLEPGPDAAAIFGGFNIRPTARVVLKAQYGHVFFPSAAPG
                     DPALTPIGLLDFQAAWSF"
     gene            3568..4059
                     /locus_tag="VFQ61_16840"
     CDS             3568..4059
                     /locus_tag="VFQ61_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956178.1"
                     /translation="MLTRRSLLWVWASGMVGLFTSSAAERARAAERTRLAVVVAKSSR
                     VEGLSLAQLRRAFLGDPLELAGKNVNPVNRANGTKERTGFDSIVLGMSPEEVPRYWID
                     RKIRGQSGAPKAVEPSELHQRVVAKVDGGIGYVIPSEVRDDVKVVRVDGKLPNEKGYP
                     IEF"
     gene            complement(4132..5571)
                     /locus_tag="VFQ61_16845"
     CDS             complement(4132..5571)
                     /locus_tag="VFQ61_16845"
                     /inference="COORDINATES: protein motif:HMM:NF015390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HET9956179.1"
                     /translation="MTHRGISCYSAAIVLSAVGACEPDVASLSIHYGTGTSEGGTVSS
                     GMPPSRGGASSSETGGGGKQGEGGGTEDGGQAGSPMGGSTHEPDPCSDLRRNAGETDV
                     DCGGDTDCPRCEVDARCKSNSDCDTDLCISSRCSEPSCDDRVQNQDETGIDCGGVCSD
                     RSRCSLESSCRVHTDCESEFCSARVCRDHCDSRHREADETDVDCGGASCDRCPEGKAC
                     TQRKDCQSDVCTNGKCVAPSCEDDLRNQNESDIDCGGVCAPTHACGMGRRCNSASDCE
                     TYVCAMNRCANDITVALEDVIDDFDDGDLSLPSHSGRLGNWFSFSDKTSQSVLKIADD
                     ARPNSKYSLVYSGEGFTDWGSGIGFDFNNPGFEEGTKVPWDGTGHSALSFWAKAAMPT
                     TIKVQLADRNTHFRGGVCTTCGHYWFKSIGISDEWKRYTIPFIDLVEEVGTEPPPPAF
                     DVTGIVMFQIFMTAGIKFELAIDDIALLR"
     gene            complement(5745..6086)
                     /locus_tag="VFQ61_16850"
     CDS             complement(5745..6086)
                     /locus_tag="VFQ61_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956180.1"
                     /translation="MATLRIISIFASLALLVGACSAGAEHETTDGMSEVTPSARTEEL
                     KVCLDNVLCIQGTRWDATACKCVPDLGDFCISGPGGPCGGFTPNPCMCQAGLKCVPSR
                     IPDVPGRCTRL"
     gene            complement(6328..>6938)
                     /locus_tag="VFQ61_16855"
     CDS             complement(6328..>6938)
                     /locus_tag="VFQ61_16855"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9956181.1"
                     /translation="RELLLSLREPTNLSDPVGPAWVEEAGRDFTALGGKAVLTLVTVA
                     VVGHLLLRRKPRSAVFVAISVVGGALLSSALKGIFVRPRPALVTHLAYVTSSSFPSGH
                     SMLSSAVYLTLAGLLARSEASLVRKAYILLWATILALLVGFSRVYIGVHWPSDVLAGW
                     AAGAAWAAFCWTVARALQRSGDVEPPEPSPADSSQEERADSQ"
BASE COUNT         1330 a         2144 c         2140 g         1324 t
ORIGIN      
        1 tcgccaagaa catcgatttt cgggccgctt gcggttgctc ggattgggct gtgttgggtg
       61 acttggggct catgagtaac gcgctcatga atctttgctt gaactcagcc gacgccatca
      121 ccgagcgcgg cacaatcgtg atcgagacta aactgatcga actcgccgaa gcggcagctt
      181 tggccttcgg agtccctcct ggaagttatc tggagctcgc ggtgcgcgac gacggatacg
      241 gcatggctcc cgaggtcttg gcgcgagcct tcgagccgtt cttttcgacc aaagatagca
      301 aacgacgctc cggcctcggc ttgccgatgg tctacggtgc cgtgcaacaa caccggggag
      361 gcctgaagat cgagagtgaa cccgggcgcg gcactcgggt gcagatcgtg ctgcccgcgt
      421 atcagcggct ctcggcccca gcgccagaga aggcgaagaa gactccagtc gtgagttcct
      481 cgcgcccgct cgcgctgttc gtggatgacg agccgctatt gcgcaaagcc ggcaaacgca
      541 tgttgagcgg actgggttac gaagtggttc tcgccagtaa cggccacgac gccgtgcaga
      601 aattcaacga gcaccgccac cgcattggcg tggtcatcct cgacgtcgcc atgcccgtga
      661 tgggcggggc cgaatgttgc gagcagctgc gcaaagtaga tcccgatttg cctgtcgtgt
      721 tcgcctccgg ctttcccaag ggccacgacg ttcaatccct aacttcgttt cccaaaactc
      781 gctacgttca taaaccttac gagttggatg acatctccaa cgccttggca gagttggcgg
      841 aagcatttcg accgtccgtg cggctcgcct gccaagccaa tctggtacgg attcaggacg
      901 cccgcgcata gaacctatcc cacagtagta gcgccggaga ctagctccta ctgggatggg
      961 ttctcaaagg atgaaggcgc gccgggcgct tccgcggctg ctagttcgaa gccggcaatt
     1021 cctcactgac tcccaccgcg tctgaagcac gcggcgcccg accgccccgg cgcacgcggt
     1081 gggccgttct tggcagtcga accaagtcga aaatggggtg acgccgagcg cgggtggcga
     1141 actccgactc tggtgtcggt tgccgccgtc gagatcggaa ctgatcgaac gcaatttaat
     1201 gttcgcgaga cacgagacaa ttgccaaaga gtctgattag actcgaccgc gcttcagaac
     1261 tcggtttgaa ggttcagcca tattcgcgga agggaactcg atgcgagcta tcgccgggaa
     1321 accgatagga gcttggcgaa gcctgcgtgc tggcgcgggc ttactcatgt tctccatcat
     1381 tcttcctgtc tcgtgcgttt acgatacttc ggatcgatgc ggtccgaatc aacgcctcga
     1441 tgacaagtat gaacgctgcg tttgcgttga tggatctgcg tttacccccg gtgggtgcgt
     1501 ggcgtgcggt cccaacgaga cggccggcgt ttcgggttgc gaatgcaaag cggggtacag
     1561 ccgcccggct gcgggcggag aatgcgaaga ggcacccgcg ggtctgggaa gcgactgcgg
     1621 taccgaaagc ccgtgcacgg atcccgctta tgatcactgt gagatcaaag ccggagcgag
     1681 taccggctat tgcacgatcg ccggctgcga cccaggcagc tgtgagggag gctacgcctg
     1741 cgacgaccga agctcgccct cggtttgccg acggcctccc ttggggcagg gcaagagttg
     1801 ctccactccc gcagactgcg cgggcaccga agcgaccttt tgcgacacag ttgggaccca
     1861 cggctgcgtc gttcagggct gcacggtgga cccgaacgat tgctttcccg gttgggactg
     1921 ttgcgacctg acgcggttcg gggttccgca accgatctgc gtgccgcaag gagggtgccc
     1981 cgcatgagcc cgtgcaaacg cagttggaca cggagaaact gggcattctc gctcgtgtct
     2041 gctctcagtt gtgcgacggc gctcgatgcg cgcgcgcaag cagctcctcc cgctccggcg
     2101 cctaccgagc ccgcgcgacc agcaccggct caacccgcac ccgacgcgcc tcccgctgcg
     2161 aatccggttg ctcccgcgcc cgacgccaaa cccacgcctc taccgggagc gccggctccc
     2221 gccgggctcg gctctgctcc tgcggatgcc aatacagagg tggcgggtag taatgcagct
     2281 acggaacctg catcagcgga ggcggggacg ccaggcgcca cagccggagc tgcagaagcc
     2341 gaggcggacg ccgacgccgc ggcggccgcg ttcatggcct ctcaagaggt ggacgccggc
     2401 gctaccgcga atagcgacta caagataaac atctacggct ttgccgattt cacgtactca
     2461 gtcaccgcca acaagaacga attccgcgtc accaagccct cgttcatggt gggcaacttc
     2521 aacgtttacc ttggcggcga gctcggtgat ggttggcgca cgctgacgga ggtgagatat
     2581 ctatatctgc cgcacggcac cccgccggcg agtcaatcgt tcgcagtcgc ccctgagcgg
     2641 atcgatacca cgattcagga tcacgccgac gtgcgcggca cgatccgttg gggagggatc
     2701 agcatccaac gcgtatacgt cgagaagacg attaaaccct ggttgaccgt gcgtggcggc
     2761 caattcttaa cgccctacgg gatttggaat gtggaccacg gctcgcccgt gattatcggc
     2821 gttcgccgac cctacatcat tggcgagacg gtattcccgg cacaacagac cggtctcgaa
     2881 gttttcggaa gccagctgtt cggcgacctg gagctaggat atcacctcac tctttccaat
     2941 gggcgcggtc cggtcgacgc gtacatggac ctggacggaa acaaggcggt gggtgcacgc
     3001 gcatggggcc gctaccgttc ggagctgggc atcgtcacgt tgggattctc aggctatcgc
     3061 ggcaaataca cgaaccgaac acagcgcttc aatttcggcg ctgacaactc attgcaggtc
     3121 acccagccgc tcgttgaaca atacgacgaa ctcagcttgg ccgtggatct gaagtgggaa
     3181 cgcaaggggt tcctctttca gacggaagct atgctgaacg acgtttctta caacgaacgt
     3241 gtccggcctc agtgggcttt cgtcacgtcc gggccacctg gcttcaaacc tgactaccgc
     3301 cgacgtggat tctacacgtt agtcggatat cgattcgatt tcttcgggat catgccgtgg
     3361 gttcaaggcg aatacttcaa ttcggggtta ctggagcccg gtcccgacgc tgcggcgatc
     3421 tttggtggtt tcaatatccg accgacggct cgcgtcgtgc tcaaagccca atacgggcac
     3481 gtcttcttcc catccgcggc gcccggggat cccgccctca caccaatagg tcttttggac
     3541 tttcaagccg catggagctt ttgagtcatg ctgaccagaa gaagtctcct ttgggtctgg
     3601 gcttccggta tggttggcct gttcacgagc tccgccgcag agcgagcgcg agcggcggag
     3661 cgtactcgtt tggcggtggt ggtcgcaaaa tccagccgcg tcgaggggct ctcgctggca
     3721 cagctgcggc gtgcgttttt gggcgatccg ctcgaactcg cgggtaagaa cgtcaacccc
     3781 gtcaatcgag cgaacggaac gaaggagcgg acgggttttg attcgatcgt gctcggtatg
     3841 tctccggagg aagtgccgcg ttattggatc gaccgcaaga tccgcggcca atctggcgca
     3901 ccgaaggcgg tcgagccgtc agagttgcat cagcgcgtcg tcgcgaaggt ggacgggggt
     3961 ataggctacg tgatccccag cgaagtgcgc gacgacgtca aggtcgtgcg cgtggacggg
     4021 aagctgccca acgaaaaggg ctatccaatc gagttctgag cggcaggggc tgctccgcga
     4081 agtcgtggag agaatcagtc gggaggcggc ctcaggccgc ctccccgctg ttcagcgcag
     4141 cagcgcgatg tcgtcaatcg cgagttcgaa cttgatgccc gccgtcatga agatctggaa
     4201 catgacgatg ccggtgacat cgaatgccgg gggaggaggt tcggtgccga cctcctctac
     4261 cagatcgatg aatgggatcg tgtagcgctt ccactcgtcg gatatgccaa tggacttgaa
     4321 ccagtagtga ccgcacgtgg tgcaaactcc accccgaaag tgcgtgttac gatccgccag
     4381 ctgcaccttg atcgtggtgg gcattgccgc cttcgcccag aagctgagtg cgctatggcc
     4441 agttccatcc cagggaacct tggtgccttc ctcgaatccc gggttgttga aatcgaaacc
     4501 gattccgctc ccccagtcgg taaagccttc gcccgagtag acgagcgagt acttggagtt
     4561 cggacgcgca tcgtccgcga tcttgagcac gctttggctc gttttgtcgc tgaaggagaa
     4621 ccagtttccc aagcgcccgc tgtgggacgg caggctcaag tcgccgtcgt cgaagtcgtc
     4681 gatcacatct tccagcgcaa ccgtgatgtc gttggcgcag cggttcatcg cgcagacgta
     4741 agtctcgcaa tcgctcgcgc tgttgcaacg ccttcccatt ccgcacgcat gagtgggcgc
     4801 gcagacgcca ccgcagtcga tatcgctttc gttctgattg cgaaggtcgt cttcacaaga
     4861 aggcgccacg cacttgccgt tggtgcagac gtcactctgg cagtctttgc gctgagtgca
     4921 ggctttcccc tcggggcagc ggtcgcacga ggcgccgcca caatccacgt ccgtttcatc
     4981 tgcctcgcgg tgccggctgt cgcagtgatc gcggcaaacg cgcgcggagc agaactcgct
     5041 ctcacagtcc gtgtgcactc ggcagctact ctcgagcgag cagcgtgagc ggtccgaaca
     5101 caccccgcca caatcgatgc cggtctcgtc ctggttctga acgcggtcat cgcagctcgg
     5161 ttcgctgcag cgactagaga tacataaatc ggtatcgcag tcggagttgc ttttgcatct
     5221 ggcgtctact tcgcaacgcg ggcaatccgt gtctcctccg cagtcgacgt cggtctcgcc
     5281 tgcgttccga cgcaagtcgg aacagggatc tggttcgtgc gtgctcccgc ccattgggct
     5341 gcccgcttgg ccaccgtcct ccgtcccgcc accctctccc tgtttaccgc cgccgcccgt
     5401 ttcggagctg gaagccccgc cacgcgaagg gggcattccg ctggacacgg tgcccccctc
     5461 gctcgttccg gtgccgtaat ggatcgatag agaggcgacg tctggctcgc aggcgcctac
     5521 ggcggataga actatcgcgg ctgaatagca agagatccct cggtgcgtca tggaacggct
     5581 cggcgagcgt gccaagacgc agtggcgagc ctcgggtcat tccgtcatcc aaagcgcgct
     5641 cgacgtgaga accctcagct ccgttcggac cagtattgaa cctcaagtga ggttgaggga
     5701 agccgcgacc gccattatcg gtcgaacgtt agtggctcca cgctctataa gcgggtgcag
     5761 cgcccaggta catcggggat acgactggga acgcacttca acccggcctg acacatgcaa
     5821 ggattcggag tgaagcctcc acaggggcca ccgggcccgg aaatgcagaa gtcgcccaaa
     5881 tcggggacac acttgcacgc ggtggcgtcc cagcgggtcc cctggatgca cagcacattg
     5941 tcgaggcaaa ccttcagctc ttcggttcga gctgacggcg tgacctcgct cattccgtca
     6001 gttgtctcat gctccgcgcc cgccgaacag gcaccgacta gcaaagcgag cgacgcaaag
     6061 atggagatga ttctcaacgt ggccatcaat cctccctttc gagtcgacgg tgggtcgcca
     6121 aagtggcaac gcttccatgc cgccgtttgg tatctaccct atgcggcata cgcgctgaac
     6181 gcgcaactcg ccaccatcac gcgattccca tgtggccacg cattcgcgtg ctcgagtagc
     6241 caagtggccg catcgacgtg tgagtaaggt gatgactatt ccacgaattt atcgctatgt
     6301 accgggctcg ccgttgcgcc cctctgctta ttgggaatcg gctcgttcct cttgcgatga
     6361 atcagccggc gagggctccg ggggctcgac atcaccgctc ctttggagcg cgcgagccac
     6421 ggtccagcag aatgccgccc aagccgcgcc agcggcccag ccggcgagca cgtccgaagg
     6481 ccagtgcacg ccaatgtaga cgcggctgaa ccccacgagc agggccagga tcgtggccca
     6541 caataggatg tacgctttgc ggacgaggct ggcttcgctg cgcgcgagta gtcccgccaa
     6601 ggtcaagtac accgcggaag acaacatcga gtgaccgctc gggaagctcg aggaggtcac
     6661 ataggcgagg tgcgtgacga gcgctgggcg cgggcgaacg aagatccctt tcagagcgct
     6721 cgagagcagg gcgccaccga ccaccgagat ggccacgaag acggccgacc gcggcttgcg
     6781 ccgcagcagc agatgaccca ccaccgccac ggtgaccaaa gtgagtacgg ccttgccacc
     6841 gagcgcggtg aaatctcttc ccgcctcctc gacccaagcc gggccgacgg gatccgaaag
     6901 gttggtgggc tcgcggagcg agagcaagag ttcgcgat
//
LOCUS       DASTKU010000488         4617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1432741, whole genome shotgun sequence.
ACCESSION   DASTKU010000488 DASTKU010000000
VERSION     DASTKU010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432741"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFQ61_16860"
     CDS             <1..451
                     /locus_tag="VFQ61_16860"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9956182.1"
                     /translation="VEVPALRERGNDILLLAQFFLERAAQRSSKPVTRLGRLVADRLL
                     SYDWPGNVRELENCMERAVAVARFDEITLDDLPPPLREVGPSTDLFETTEDPKQLPRM
                     NVVEERYIRKVLSAVGGNKTLAAKVLGFDRRTLYRKLERATNSTRPE"
     gene            604..1521
                     /locus_tag="VFQ61_16865"
     CDS             604..1521
                     /locus_tag="VFQ61_16865"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9956183.1"
                     /translation="MARLLHRRTQELILVEPELLIGRSAACGLRIREPYVSAQHASLR
                     WSGWRWEIKDLGSRNGTFLNGARVEVGRAHPVERNGVLGFGSESEAWVLSDDSAPEAM
                     AIPLDGGEPVTPENDMIALPSLQSPEASIFRDADGLWKLENLDGERIVLEHHKLFQVG
                     GHPWRFASPDIIGPSSSALVPSQARPLKLRFSVSPDEEYVEIRAEYGSTVFDLGSRSH
                     NYLLLTLARVHLEDSKNGLPPHSCGWIHQEDLLQKLHITQTQLNIDVFRIRQHFAKVG
                     IPGNINVIERRPRNKQLRLGIAVFDIEVA"
     gene            2210..3013
                     /locus_tag="VFQ61_16870"
     CDS             2210..3013
                     /locus_tag="VFQ61_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9956184.1"
                     /translation="MGFPAESMELEAPYASDRAGLGELARYAAVRGDLTASSEMDLGL
                     VWSNLSSGHFRVVDWFFTKSRTFMVLETLERRERRLTKRKLEVLERILLRGGQKAVAV
                     ELNLAPSTVAIIAGNCLRTLGIDSGASRAPVLLSAAVHAFHGRTPLGRGQKSLLSHSG
                     RTYQVISMARPEAWLVNELSPAEFAVTRLLVEGHSHAEMAALRHTSVRTIANQLAAAF
                     HKLGVSGRCELLCRTLRASFEATAWPPPGSSPGDRLAAAAGDSVRLETA"
     gene            3244..4353
                     /locus_tag="VFQ61_16875"
     CDS             3244..4353
                     /locus_tag="VFQ61_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9956185.1"
                     /translation="MSPRGLAVIGGGLAGLSVAVMRAQAGGAVHIFEGSPRFGGQLQT
                     EQHAGFVVEQGAEGFVARSEAVPRLARSLGIEGELVDQSVHRSFAWDGQALAELSPGE
                     AARLLGFQVPVEELGKGIRSFRWGMEQLARALVARLRSPELASRVELVTNAPVRSVRK
                     SSGGVRLVFEPDARSAEFDQVVIATHAAGAARLLDAELGGVSAALAGSRTLSSVTVSL
                     AFESDGVRYPNEGSGFIVLPEACEQAFRACSFSSCKLPNRAPPGKHLLRVFLRPDEHQ
                     LQAVGDAELASMAQSCLERALGQRIDPLWSWVSRWANALPVFDPPHTAHVAALEEALL
                     SEFGHARVLLSGAAFHGSGIDAAVRSAERVARALA"
BASE COUNT          890 a         1399 c         1488 g          840 t
ORIGIN      
        1 cgtggaggtg cctgcactcc gagagcgcgg caatgacatc cttttgctcg ctcagttctt
       61 cctggagcgg gccgcgcaac gcagcagcaa acccgtaacc cgccttggcc gcctggtggc
      121 cgaccgtctg ctcagctacg actggccggg gaacgtgcgc gagctcgaga actgtatgga
      181 gcgcgccgtc gcggtcgcgc ggttcgacga gatcacgctg gatgacttgc cgcctcccct
      241 gcgtgaggtc ggtccctcca cggatctttt cgaaaccacc gaagatccca agcagctgcc
      301 acgcatgaac gtcgtcgagg aacgttacat ccgcaaggtc ttgagcgcag ttggcggaaa
      361 caaaaccctg gccgcgaagg tgttgggctt cgatcgccga actttgtatc gcaagctcga
      421 gcgcgctacc aactcaaccc ggcctgaata ggtagcaatt tcgataactt gcagccggcg
      481 gtcgtgctcg atggatgggc attgaccgcc ggtttcgtta gcaggcccgc cgcgagttga
      541 tttgctggcc tgcccgatgg gataaaggag aacgcggtta cgctcgctcg cctgcgcgac
      601 tccatggccc ggctcctcca ccgtcgaacc caagaactga ttctggttga gccagagctt
      661 ctcatcgggc gctctgcggc ctgcgggctg cgtattcgcg aaccgtacgt gtcagcgcaa
      721 catgcctcgc tgcgctggag cggctggcgc tgggaaatca aagacttggg gagtcgaaac
      781 ggcacgtttt tgaacggtgc ccgtgtagag gtcgggcgcg cgcacccggt cgaacgcaac
      841 ggcgtgctcg gcttcggaag tgaatcggaa gcctgggtgc tcagcgacga ttccgcgccc
      901 gaagcgatgg ccattccgct cgacgggggc gagccggtca cccctgaaaa cgacatgatc
      961 gcgctgccga gcctgcaaag ccccgaggcc agcatctttc gcgatgcaga cgggctgtgg
     1021 aagctcgaga acctcgacgg agagcgcatc gtactggagc accacaagct ctttcaggtc
     1081 ggtggccatc cctggcgctt cgcgagccca gacatcatcg ggccttcctc ctccgcgcta
     1141 gtcccctccc aagcgaggcc cctgaagctg cgcttctcgg tttcacctga cgaagagtac
     1201 gtggagatcc gagcagaata cgggagcacc gtgttcgatc tcggcagtcg cagccacaat
     1261 tacctgctgc ttacgctcgc gcgggttcac ctggaggact cgaagaacgg cctcccgccg
     1321 cacagctgcg gctggatcca ccaggaagac ttgctccaga aactgcatat cacgcagact
     1381 caactcaata tcgatgtttt ccggattcgg cagcacttcg ccaaagttgg cattccgggc
     1441 aacatcaacg tgatcgaacg acgcccccgc aataagcagc tcaggctcgg cattgcggtg
     1501 ttcgacatag aagttgcctg atctttggca ggttgctcgg gcgcggcttc acgttgacgg
     1561 ctaacacacg tgagtcacat gctatgctgc tcggcaggga cggatgtgca tggaacgccg
     1621 agcagcaacg cgaaaactag cccgaggatt ctgaggccgt ctcgagcatc tgcgcagcgc
     1681 tgtaggcgta acaggaatgg aagtaaccgg cactgcgtac gtcagccgag ggccgggccg
     1741 gtgggcaagg cacaaaaaac ctcgagaacc cgcgagatgg agcgcttggc gcaagggccg
     1801 cacgtaggaa atccgttgta atcgcgtgta atcgacaggc actccggaac cctgtcataa
     1861 cctccgatac aaggaggtcg ggcggcgctg tcaacgagcc ctgcacctac ctccggatgg
     1921 cgaaaactgg gcctgagcca ggatttctcg agttcgcggg cgtgtacatc aacgtgtaac
     1981 gagcgtgcga gcagcacgcg aagacgaacg taagcagcca cacccttggt gactgcgcgt
     2041 tacgaaaaga tttcgagaaa acacgttaca ccccgcgagg gcgagcaatc gggtcgaggg
     2101 gaactcgccg agctacggcg ctcgatgagt tcagaaaaag ggtctggccc caacacgaaa
     2161 cacgcaaagc cctgtgcttg cgtcgagagt gcgaatcaag gagctcatca tgggatttcc
     2221 ggctgagtct atggaattgg aggcgcccta cgcatcggat cgagcgggac tcggggaact
     2281 cgcacgttac gcggcggtgc gcggcgattt gacggcatcg agcgaaatgg atctgggctt
     2341 ggtctggagt aacctgtcct ccggtcattt ccgagtcgtg gattggttct tcaccaagag
     2401 ccgaactttc atggttctcg agactctcga gcgacgcgag cgcaggttga ccaagcgcaa
     2461 gctcgaagtt ctggaacgca tcttactgcg cggagggcag aaagcggtcg cggtcgagct
     2521 caacctggcc ccctcgacgg tggccatcat cgcgggtaat tgcctgcgaa ccctcggcat
     2581 cgacagcgga gcgtcgcgcg cccccgtgct gctgagcgca gccgttcacg ccttccacgg
     2641 gcgcacccct ctgggccgcg ggcagaagag cctgctctcc cattcgggtc gtacctatca
     2701 ggtcatcagc atggcgcgcc ccgaggcctg gctcgtcaac gagctatcgc cggcggagtt
     2761 cgccgtgacg cggctgctgg tagaggggca cagccacgcc gagatggcag cgctgcgcca
     2821 cacttcggtt cggacgattg ccaaccaact cgcagcggca ttccacaagc tcggtgtttc
     2881 agggcgctgc gagcttctct gccgaaccct gcgggccagc ttcgaagcta ccgcttggcc
     2941 acctcccggc agctccccgg gggatcgtct ggcggctgcc gccggtgaca gcgtgcgtct
     3001 agaaaccgcg tgagagaatg aggcctggtc agcgggagcc aggccgaaac tgatctctgg
     3061 ttggggggcg taggatgcga atgggtacag ggcatcgagc cacagtgggg agtgtgatga
     3121 gcagagcaaa tgaatgcgaa gagcatgttc tccagtcggt gtgagatatg ctcttcgcat
     3181 gccatttgcc cctaacgagc cgaatactcg cttccaggcc cgcagcgcag caggaggcgc
     3241 ctgatgtctc ctcgcggctt ggcggtcatc ggaggcgggc tagcggggct ttcggtcgct
     3301 gtgatgcgag cccaggcggg tggtgccgtg cacatcttcg agggctcccc gcgctttgga
     3361 gggcagctac aaactgaaca gcacgcgggt ttcgtcgtcg aacaaggtgc cgaaggtttc
     3421 gtggctcgca gcgaagctgt gccgcgtttg gcccgttccc tcggtataga aggcgagctg
     3481 gtcgaccaaa gcgtgcatcg ctcgttcgct tgggatgggc aagccctggc ggagttgtct
     3541 ccgggtgaag cggcgcgtct gctcggcttc caggtccccg tcgaagagct cggaaaaggc
     3601 atacgctctt ttcgttgggg catggaacag cttgcgcggg ctctggtcgc tcgtctgcgt
     3661 tctcctgaac tcgcgagtcg cgtcgagctg gtgacgaacg cgcccgtgcg ctcggtccgc
     3721 aagagtagcg ggggggtacg gctcgtattc gagccggatg cacgcagcgc agagttcgac
     3781 caagtggtga tcgcgaccca cgccgccggc gctgcccgac tgctcgacgc agagctgggc
     3841 ggtgtctccg cggcgctggc cggctctcga acactgtcga gcgtgaccgt gtcactcgca
     3901 ttcgagagcg atggggtacg ctacccgaac gaaggcagcg gattcatcgt gctgcctgaa
     3961 gcgtgtgaac aggctttccg tgcttgtagc ttcagttctt gcaaattgcc gaaccgcgct
     4021 ccaccgggca aacacttgct gcgggtattt ttgcgccccg acgagcacca gctccaagcg
     4081 gtaggagacg cggagttagc gagcatggct cagagctgtc tcgaacgggc gctgggccag
     4141 cggatcgacc cgctgtggag ctgggtatct cgctgggcca acgccctacc cgtcttcgat
     4201 ccgccgcaca ccgcgcacgt ggcggcgctg gaagaagcgc tgctgagcga attcggtcac
     4261 gcgcgagtgc ttctgagtgg tgcggccttc cacggctcgg gcatcgacgc agcggtacgt
     4321 tccgcggaac gcgtggctcg cgcgctagct tgaacggatc gcaaggcaac cgaccacaca
     4381 ggcgcggtcg taatgctgag gctcagccaa gcggcagagc tctctaccaa gcggtagggg
     4441 tacggctcga tcgcctcggc ggcttcattg attgagcgac gactcggcta acccagcggg
     4501 tgttacaatg tgcgcgccgc atctctatag gcgggccgac ttatcgcggg ccagccaaag
     4561 gatcacggca tcacggcaca cggcacgaag caccaaagca cgtaagcgag ctgaatg
//
LOCUS       DASTKU010000489         6639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1879092, whole genome shotgun sequence.
ACCESSION   DASTKU010000489 DASTKU010000000
VERSION     DASTKU010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6639
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879092"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VFQ61_16880"
     CDS             <1..1071
                     /locus_tag="VFQ61_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956186.1"
                     /translation="RLAGLTDGFAFFFAGGHLGRVSVTGGAASALANVAGTRVQVLRE
                     RLYLEIGGSVYSAPLRGGAFQYITHLPAGGSELFERAGRLYALTRDVLLRFDPAGARP
                     LRLFDTQSGAASSTQVDPIVVASLTQAEANLLVTLGKPGDIRELWKVPKRDGTPARLW
                     QGERIEAAPVVDERFVYLLAGGALERMPWAGGPIEPLVSAEALRAAFRTTSAVAERPE
                     QSEVLLPHRMRLEGEFVYVDDERGYAAVQARKTGGIAGVLPGSSARAAPVDAKELSAE
                     EGGARNAYTAGDTVFFTTPRHEIRSRSLVTGAVRILTVAHHPAALIADDVVLYYLDIG
                     ERGSVVAQFARVGCCAIWSVPR"
     gene            1858..2760
                     /locus_tag="VFQ61_16885"
     CDS             1858..2760
                     /locus_tag="VFQ61_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956187.1"
                     /translation="MKARITTALGASLYLVGFTLLPATAHAKALDKCGSIFLSASSSC
                     EFKPIQECMTTCNTTSTEQVCAQKTFSMCSSSCASTDTTTCTQTHTDSCMKDCETIAT
                     KSSHEVCVNDCTDRCTNDAVAKGRFGGNHGTCGRNCSHDCEARCDSSSTSDQSTDCTT
                     KCTSVVQNECTEEVTRDCVLSCQTEHYTDCQTQTVNTCTTSCQNKGGAIFCDGQYLEA
                     SDLQACADQLAEEFSFNIDVSVSVKVNGNGSVTTTNGDGTKTTKASCAFSPDVGSGSS
                     TGGGVAIGALAMLGLIVNRRRRRG"
     gene            complement(2992..4857)
                     /locus_tag="VFQ61_16890"
     CDS             complement(2992..4857)
                     /locus_tag="VFQ61_16890"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9956188.1"
                     /translation="MNRVQTSLTSLTRTCLAVLVAACSSGQSNSPADGRAADGGSGGS
                     AATQSGGSAGDAPKGGSSATQSGGGAGAAKGGSAATQSGGTAGDNPKGGSSATQSGGG
                     AGAAKGGSGGATGSPPDKPGPKSDFTEQVGNVSFDMVYMPGGTFTLGCESGSCPQYAE
                     PVAGVKVSGYYIGKTEVTTGLWNTVMGGTSAGLAGPSGSITSITWYDAMEFSCKLSQM
                     TGRKYRMTTEAEWEYAAKKHSSSLEKIGSGEEWAYNSWDTKHTGGTDPVGPNSGAHTQ
                     KTRRDAQGTADNITGRLIRSIDGIGPALRLAIADEMAFPPGYVPPCELHAPSVGDEPV
                     NSYRDPRWVTGSDAHWTAGSIAIGSFDLRVWEDGTARLGSTNGQWFTSNNIAFVFVPS
                     SGSSKKFPYIFLDKTQGSLISDISFMSGGFVGRIVKESTTNFEKPTLSGLKSGAELAA
                     AAGDDYKMVDMANIPESAKKQDPRLLDGPSQGWFQDNRSAGGVHHYRKDVDADEFRFT
                     VNQGTSRTMLANGKWFTVNDTFLRVTHASGYTTDYLYAIDADGTFYHDSFQAYERADF
                     RMFKKTANGSDTFAATCGSICSDEIPKGEPASLYAKMDNGKSTFVPALCPADGCK"
     gene            5853..6488
                     /locus_tag="VFQ61_16895"
     CDS             5853..6488
                     /locus_tag="VFQ61_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956189.1"
                     /translation="MSELSIEFGDGECGLSSKPGCKLLTKMHGRAVRRLSGWVFLHVF
                     AAALSLRAAAEPEPRTVRLSPRPPPSRASVATIDFARFYRFTNRAPVPTPELLELAGK
                     RVTLVGFMVALERPVLGGFYLSPYPASSDESGAGRGGVPPTAVLVLVRSARGRYVQHV
                     PGALELTGVLEVGNQETSGETSTIRLRIDDARALRFARTRATLRHRREAAK"
BASE COUNT         1144 a         2074 c         2152 g         1269 t
ORIGIN      
        1 cgcttggcgg ggctgacgga tgggttcgcg ttctttttcg cgggtggtca tctcgggcgt
       61 gtgagcgtga cgggaggtgc ggcatcggcc ttggcgaacg tggctggcac gcgcgtgcag
      121 gtgctgcgtg agcggctcta cctcgagatt ggcggctcgg tttactccgc gccgcttcga
      181 ggcggcgcat tccagtacat tacgcatttg ccggccggag gctccgagct gttcgagcgc
      241 gccgggcgct tgtacgcgct gacgcgcgat gtactgctgc gcttcgatcc ggcaggcgcg
      301 cggccgctac gtctgttcga cactcagtcg ggcgccgcgt cttccacaca agtcgacccg
      361 atcgtggtcg catcgctcac tcaagcagaa gcgaacctgc tcgtgacgtt ggggaagccg
      421 ggcgacatcc gcgagctctg gaaggtgccg aagcgcgacg gaacgccagc gcgattatgg
      481 caaggcgaac gaatcgaggc ggcgcccgtc gtggacgaac ggttcgtgta cttacttgcc
      541 ggcggcgcgc tcgaacggat gccttgggcg ggagggccga tcgagccgct cgtaagcgcg
      601 gaagcactgc gcgccgcgtt ccgcacgacg agcgcggtgg cggagcgccc ggaacagtcg
      661 gaagtgttgt tgccgcatcg aatgaggctc gaaggcgagt tcgtgtacgt cgacgatgag
      721 cgcgggtacg cggcggtgca ggcccggaag acgggcggca tcgccggcgt gctgcctggg
      781 tcatccgcgc gagccgctcc cgtcgatgcg aaggagctct cagcggagga gggcggcgca
      841 cgcaacgctt ataccgcggg cgacaccgtg ttcttcacga cgccgcgcca cgagatccga
      901 agccggtcgc tcgtaaccgg cgccgttcgc atcctcaccg tggcgcatca cccagcggcg
      961 ctgatagccg acgatgtcgt cctctattat ctcgatatcg gagaacgggg aagcgtggtc
     1021 gcgcagttcg ctcgcgtcgg gtgctgcgcc atctggtccg tgccgcgctg aagccgagcg
     1081 ggcccaatca cggcgtcacc cggcggagcg gtctcagcac gcggatgctc gcgagcgagg
     1141 aagcgtcgcg cgcgtcgacc tcgaccgtcg ctcccggatg accgaacgtg tatcgcttcc
     1201 gggtgacgga gcccgcgagc ggccgatagc accgtcgcgc actgccggcg tggccgcgag
     1261 atcgcgacgg agcgctctgc gacacgccac aggcagccgc ttcagcttca actccaggat
     1321 cgtgatgcga tcgatgcgct cacccggcga aacctccagg cccttacgct tcgtcgcgcg
     1381 cgttggtgag ttcggcggat atccggtcac tcgcgctgaa ggagctcttg agtggtttca
     1441 gcgaatggaa agcgggcgcg aggcaacgat ggtcgaaggc agaaacgagg agagctcgcg
     1501 agagcaaggg cgcgagcaag tgggtggagc gcctgcggtc gcgactcgag tgctcgttgt
     1561 ggcggaggga gggccgccgg gtgatgcgtt acccaggcgg cggggcttgt gtctgccagt
     1621 cggccccgca tcgtggcagg gctgcacttt cgggcgcgct cgcgcttgcg catagtgctc
     1681 gcgagctaaa tcagcccaga gcgtgcgttc ttccaatgaa aacgcgagcg catccgcgag
     1741 cgcacgcgcc tcaacggcag ccgcgaggcg attggggggc gattggtacg ctagctgcaa
     1801 gttgcgtcct cggtcggcga attgccagcg gccactttcc ctctcgagga tttcacaatg
     1861 aaagctcgca tcacgactgc tttgggtgca tcgctttatc tggttgggtt cacgctctta
     1921 ccggctaccg cacacgcgaa ggcgctagac aagtgcggca gcatcttcct ctccgcctct
     1981 tcttcttgcg aattcaagcc gattcaagag tgtatgacga cgtgtaacac cacgtcgacc
     2041 gagcaagtct gtgcacaaaa gactttctcg atgtgcagct cgagctgcgc ttcgacggac
     2101 acgacgactt gcacgcaaac gcacaccgac tcatgcatga aagactgcga gacgatcgcg
     2161 acgaaaagct cgcacgaagt gtgcgtgaac gactgtacgg atcgttgcac caacgacgcc
     2221 gtcgcgaagg gtcgctttgg cgggaaccac ggcacctgcg gcagaaactg ttcgcacgat
     2281 tgcgaggcgc gctgcgatag cagcagcacg agcgatcagt cgacggactg caccaccaag
     2341 tgcaccagcg tcgtgcagaa cgagtgcacc gaagaggtga cgcgtgactg cgtgctgagc
     2401 tgtcaaaccg agcactacac ggattgtcaa acccaaaccg tcaacacgtg taccacctcg
     2461 tgtcagaaca agggcggcgc gattttttgc gacggtcagt acctcgaagc gagtgatctt
     2521 caagcctgtg cggaccagct ggcggaggag ttttcgttca acatcgacgt gtcggtgagc
     2581 gtcaaggtga acggcaatgg cagcgtgacc accaccaacg gcgatgggac gaagacgacg
     2641 aaagcgagct gcgcgttcag cccagacgtg ggcagcggta gcagcacagg tggcggcgtg
     2701 gcgatcggag ccttggcaat gctggggctc atcgtcaatc gtcgccggcg tcgcggctga
     2761 gcgcctcggc gtggagtagg tgtagggccg ggccagacgg attcgctgcg attcggctac
     2821 ttgcgggcaa actacgtgcg actcggacca gtcccggctc ggctcggctc ggtcgcgcgt
     2881 agcccctcgg aggagaccca tcggcgcact cacggctcgc gcggtgaaca tccgcgtcgg
     2941 agtttcacgc acggttgcgc tgggggtacc agggcgagcc gtgttgcccg cctacttgca
     3001 accatccgcc gggcagagcg ccggtacgaa cgtcgacttg ccgttgtcca tcttggcgta
     3061 gagtgaagcg ggctcaccct ttgggatctc gtcgctgcag atgctaccgc aggtggcggc
     3121 gaacgtgtcg ctgccgttgg ccgtcttctt gaacatcctg aagtcggcgc gctcataggc
     3181 ttggaacgag tcgtgataaa aggtcccgtc cgcgtctatc gcgtataggt agtcggtcgt
     3241 gtatccactc gcgtgcgtta cgcggagaaa cgtgtcgttg acggtaaacc acttgccgtt
     3301 ggcgagcatg gtgcggctcg tcccctgatt gacggtgaag cggaactcgt cggcgtctac
     3361 gtcctttctg tagtggtgca ctccaccggc ggaacggttg tcctggaacc atccctggct
     3421 gggcccatcg agcagccgtg ggtcctgttt tttggccgac tcggggatgt tcgccatgtc
     3481 gaccatcttg tagtcatcgc ccgctgctgc agccaattcc gctccgctct tcaagcccga
     3541 cagggtcggt ttttcgaagt tcgtcgtcga ctccttcacg attctcccga cgaaaccgcc
     3601 gctcatgaag ctgatgtcgg agatgagcga gccttgcgtc ttgtcgagga agatgtaggg
     3661 gaacttcttg ctggagcctg agctcggaac gaacacgaag gcgatgttgt tcgacgtgaa
     3721 ccactgacca ttggtgctgc ccaacctcgc ggtgccgtcc tcccaaaccc ggagatcgaa
     3781 gctgccgatc gcgatcgaac ctgccgtcca atgcgcatcg cttcccgtga cccagcgcgg
     3841 gtcgcggtac gaattcaccg gttcgtcacc tacgctcggc gcgtggagct cgcagggcgg
     3901 aacgtagcca ggtgggaatg ccatctcatc agcaatggcc aggcgcaacg ccggtccgat
     3961 gccatcgatg gaacggatga gccgccccgt gatgttgtcg gctgtcccct gggcgtcgcg
     4021 ccgcgtcttc tgagtatggg ctccgctgtt cggtcctacc ggatcggtcc cgccggtatg
     4081 cttggtatcc caagagttgt acgcccactc ttcgccgcta cctatcttct ccaggctgct
     4141 cgagtgtttt ttcgcggcgt actcccactc ggcctcggtc gtcatccggt acttcctgcc
     4201 cgtcatctgg ctcaacttgc aggagaattc catcgcgtcg taccatgtaa tgctcgtgat
     4261 ggagccgctg gggccggcga gcccagccga agtgccgccc atcacggtat tccaaagacc
     4321 tgtcgtcacc tccgttttcc cgatgtagta gccgctgact tttacgcccg cgaccggttc
     4381 tgcatattgg gggcagctac cgctttcgca ccccagagtg aacgtgccgc ccggcatgta
     4441 aaccatatcg aaagagacgt tgcctacctg ttcggtgaag tcgcttttgg gaccgggttt
     4501 gtcgggaggg gagccggtcg cgccgcccga gccacccttc gcagcacctg cgccgcctcc
     4561 gctctgggtt gcgctcgaac cacccttggg attgtcgccc gcagtgcctc cgctttgggt
     4621 tgcggccgag ccacccttcg cagcacctgc gccgcctccg ctctgggttg cgctcgaacc
     4681 gcccttggga gcgtcgcccg cagagcctcc gctttgggtt gcagccgagc cacccgaacc
     4741 gccatccgca gcccggccgt cagctggcga gttgctctgt cccgagctgc atgccgcaac
     4801 gagcaccgct aggcacgtcc tggtgagcga cgtgagcgac gtttgtactc tgttcatggt
     4861 cctgactcct ccgtacacga cgtgagcagc acctccgcta ggtcactttc tcatggagta
     4921 gcccacccgc ggcacttcca aactcgatcc tgaacccatc gggcagcggg gttgatcacg
     4981 atcaactgag atggcttcag acattccccc agggcgcgaa gctgccaaat ccgatctaga
     5041 aacgctcgag aggatttcgc ctgctgcgag ccagatcctg aggctgcgaa gtgcggcctc
     5101 gtcgctccgc tcctcgaaag gcttgtgtct ctctccggtg ctcactcgtc aggcgccctt
     5161 ctcggtcgcc cttctcggtc gcgcttctcg gtcgcgcttc tcggtcgcgc ttcttggctc
     5221 tcgcacgggc agaagctttg ggaacgcccc tagaactggt tctaaccctg tagacgtagg
     5281 tgcgcgatgt gggacacggc tcaaattgga acgagcgaga tggctctcct gcccagcgtt
     5341 gttcgcattt gagacccgaa ctcgtaaaaa gcgcgggaag tccctattac cagcgcggca
     5401 tggcccgtgc actaagggta gtcgctggcg gcgccgacgg gttcccccct ccccgcggac
     5461 ggtcgccagc aattactact caacggtgcg atagccctcc tctcccctcc ctcgaagttc
     5521 ccctcctcac catatcaagc cgttgccgaa cggcgcgaga ccccaagctc gcgccgttca
     5581 cctttttgtg cgctcgatac ttcgtatctc ggagaatgcg agtccgtaac cgctaccgaa
     5641 tcggcgtctt gctaccgaat aggcgtcttg cacggccggc gtccacgagt ggtgccagca
     5701 aaacgaccca aagcggctcg gaagcgctac aggagatcaa tcaagccgaa gcgaacaccc
     5761 gtgtcgagcg gcacgccccg atactcgagt agcggcccgt acgggtgatc gccgggacgg
     5821 ccgtgggtcg ctcgccccga cgtgaatccg ccatgtccga gctttctatc gagttcggag
     5881 atggtgagtg tggactctcg agcaagcctg gttgtaagct cctcaccaag atgcacgggc
     5941 gggccgtccg acgactcagt ggctgggtgt ttcttcatgt ctttgccgcg gcgttgagtc
     6001 tgcgagccgc ggccgagccg gagccgcgca cggtccggct gagtccgcgt ccgccaccgt
     6061 cgcgcgcttc ggtggcgacc atcgattttg cgaggttcta tcggttcacc aacagagcgc
     6121 ccgtgccgac gcccgagcta ctggagctcg ccggtaagcg cgtcacgttg gtcgggttca
     6181 tggtcgcttt ggagagaccg gtcctcggcg ggttctattt gtcgccctat ccagcgagca
     6241 gcgatgagtc cggtgctgga cgcggcggag tgccgccaac ggcggtattg gtgttggtgc
     6301 gttctgcacg tggaagatac gtgcaacacg tgccgggtgc gctcgagctc acgggcgttc
     6361 tcgaggtcgg aaatcaggaa acgtcggggg agacgagcac gatccgcttg cgaatcgacg
     6421 atgcacgggc gctgcgcttt gcgcgcactc gcgcgacgtt gaggcaccgg cgcgaggcgg
     6481 cgaagtagtc gcggcggaag gtggcgcgag cgctcggagc tcggaacgaa cggatggccc
     6541 tgctctggcg ggcgcaatcc ctgacgaaga cacacgacgc gaatggctgc tcggcgcgag
     6601 cggccccatc gaacgggagg aaagaatgat tcggagagg
//
LOCUS       DASTKU010000490         9161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1208553, whole genome shotgun sequence.
ACCESSION   DASTKU010000490 DASTKU010000000
VERSION     DASTKU010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208553"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFQ61_16900"
     CDS             complement(<1..404)
                     /locus_tag="VFQ61_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9956190.1"
                     /translation="MTDPQDLERLLGKEGPLARTLPGYELRPSQLEMARAVERALRDE
                     RIVLCEAGTGTGKTMAYLLPALLSGRKVIVSTATRALQEQLFWKDLPLLLRAFGLKPR
                     VALMKGLGNYVCRRRLAEFEGSEERLKPRFAG"
     gene            complement(404..748)
                     /locus_tag="VFQ61_16905"
     CDS             complement(404..748)
                     /locus_tag="VFQ61_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9956191.1"
                     /translation="MKNRPASAPPESVPWVPRRSAGGARTESTERITLRAASFEATGW
                     TLNVSRGGFRAIVECQLEKDLEYEVWIGDASSPRRACLVWCQEQSDGLIAGFKYTDVE
                     PSSPHNPAPSGE"
     gene            1345..1767
                     /locus_tag="VFQ61_16910"
     CDS             1345..1767
                     /locus_tag="VFQ61_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956192.1"
                     /translation="MPALNSGELKKALRASGFEIYRASPSEVRLAERVRENLLMDSGV
                     AVRLAEPLSVAVYVRAEGSKFSGEAAEQLYARARERAEALRVRGFEEHESRAVPIWDP
                     GDRTQLLDTWYEVCFVKPVEASELDQTLRDALSFEKTA"
     gene            complement(1818..3188)
                     /locus_tag="VFQ61_16915"
     CDS             complement(1818..3188)
                     /locus_tag="VFQ61_16915"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9956193.1"
                     /translation="MKPNILVVDDDRSFRILTETALASEGFDVRVCPTLRRAREELDT
                     AAPDVMVLDRRLPDGDGIDLLEELKSSGAAATATIVVTAYGDVDNAVAAMRAGAVDYL
                     TKPTSVADLIFKIHKVLETRGLRDRLALAKGVNREPPQVAPQSASLRRVHAELERLGH
                     SPQTPVLLLGPSGAGKQFAAELLHRVTYPDRDAPFVDVNCAALPEQLVESELFGHERG
                     AFTDAKTRRRGLIEMASGGTLFLDEVTELPDASQAKLLKFLDSGRFRRVGGQRELSVE
                     LRVVAATNRDLRAGVEGGSFREDLYHRLSVFIVNVPPLAERREDIPPLAKSFVEFFSR
                     RVNKRISGISDGALKALMRYHFPGNVRELRNIIERAVILSAGPELDESCLVIEPHRFG
                     APRNSAFFTVDLLADGVVPTLEAVERAYVARVLEHCGGRRMVAAQALEVSYPTFLKRL
                     REFGME"
     gene            complement(3185..4864)
                     /locus_tag="VFQ61_16920"
     CDS             complement(3185..4864)
                     /locus_tag="VFQ61_16920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9956194.1"
                     /translation="MTPRSAKPTAPGGAAPRAVTLAAAHARLAQWTLTVYAATALVVA
                     TLLLATLLTEKTHSEEQLEGNLRTAVTDRSHALRRYLDLLSAELVRLGSRSEIESADH
                     DDAPERRLLELAHKKSTLFDVGVAILDRDGNLIWCEPHNFLPENASFHSAPWFSKAVA
                     SEEVQVVPVAPERKDALIYVVAPAMHDERLGGVVLGAIDLSRRSLGTLRSTPPLTTAF
                     VLTHEGAVVYPADARGLSTNPALMTWLTKPREHAESMHLELGGEAMVVASAPLVGTEL
                     SYALLTPESVLFAKTRHRLYLRASVGLVLVVAPFVMLVLLLRRALKIFRRSEQEAVER
                     ERLELLGEASNLIAHEVRNALNGLRVGLDLVLKPDARGGGAHERIVRQLRDEMQRLAD
                     FTSELMLFSKGIVVHPVHLDLAESVVKFSELSLDLAAEAGCRLEVKVERAPLMAKVDP
                     ALLRVVVGNLIGNALDALSSADAPREGEVSVVLDSEPGLARLRVMDNGPGVPVPVRSK
                     LFQPFSTTKPSGTGLGLFLSRRIAQAHGGDLVLQDSAKGAAFLLTLPAEVS"
     gene            5012..6448
                     /locus_tag="VFQ61_16925"
     CDS             5012..6448
                     /locus_tag="VFQ61_16925"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HET9956195.1"
                     /translation="MRRAALGWLMGPVCWVLACSESAGPAGGVTGGNAGASNAGANAA
                     GGANAGTSASGGGSAAGGTAHAAGAGNNAGGGSNAGTGSNAAGSSNSNGGRTSGAGGA
                     PATSGAGGVGGLGGSPAAGGTFTAGGTSSGGSSSSRGGATNSGGAAGQGGSTGGAAGA
                     ASKGISIQGTQLIVDGKPYHVRGVCWNPVPKGKTHPQGLDFAGAAATDIPLMQRAGIN
                     TVRTYEPLTDVGVLDQLYAAGIRVLDSVYVYGGDSPSVVADRITKIKDHPAILAWLVG
                     NEWNYNGLYVDLPAADAQARLVDAAKRIRAIDPNHPVASVYGELPSKAVIDAMPEISL
                     WGINSYRGIDFGDLFDKWAALSKKPMFLAEYGADSYNAKASKYDPESQATAVRELTQQ
                     IADHGSAFHSGAVAVGGAIFEWADEWWKDSSGSPNAQEVGGIAPGGGPYPDQTFNEEW
                     WGVVDIDRKPRPAYDALKGVFDSLTKTP"
     gene            6682..7440
                     /locus_tag="VFQ61_16930"
     CDS             6682..7440
                     /locus_tag="VFQ61_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956196.1"
                     /translation="MSTERMLSKSPAPGELALPSAKLGGPSSGPAPRESDADDVWFVP
                     SVNQRGALPARPAAAELPQPEEEGVPMSVLERQSTRLPRLVGIAALVLCGSGLAAVAI
                     WAAANKAPDVSIHAATSLEAVAALTPPRGLSGPGASEHASASNTALTEDQSSATRSRF
                     LRHAPSAELSPARLAGEPEIVPVDELVPSPAVAPESSSLHVNVDRQNAAAPQRAALPH
                     VTPSAPSYGAKTRGLHLKSKSVFDRPIAPPDGFY"
     gene            complement(7554..7943)
                     /locus_tag="VFQ61_16935"
     CDS             complement(7554..7943)
                     /locus_tag="VFQ61_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956197.1"
                     /translation="MNRFRQRHSEAAERAAERRRREDEAPRLKDEVPTLDSLRLELQE
                     HRAGAVVAETAHVRCIPVAHAPALFELPCHDTFCKEGGHDVTRAILRALQSGATHFEG
                     EDRCEGRTGSAECQRVLKFVGQATYKK"
     gene            complement(7940..8458)
                     /locus_tag="VFQ61_16940"
     CDS             complement(7940..8458)
                     /locus_tag="VFQ61_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956198.1"
                     /translation="MTASRQGSPNALERAVESVGEAWAQDFAQSLKKEGRAVAGGWPG
                     TLSEARARVVACAAGRLGPNYSIVPEQLEALTRRAYEVAKKAWLASSEPDRDEQREDA
                     RFDTLENSERPPRAAGRARARGGAAARVQATSVQGVGLGAAEATAQVAAVLPPPPESM
                     PEPLQQATPEDP"
     gene            complement(8557..8949)
                     /locus_tag="VFQ61_16945"
     CDS             complement(8557..8949)
                     /locus_tag="VFQ61_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET9956199.1"
                     /translation="MNNRLYVGNLSFHTTEDTLQRAFAQCGDVVEAKLVLDRETGRSR
                     GFAFVVMATAEDAQRAISQMDGTDLDGRALRVNEAEARKPRGGGFGGGGGGGGGFGGG
                     GGGGGRGGDRGGDRGFRGGGGSERRNRW"
BASE COUNT         1665 a         2998 c         2916 g         1582 t
ORIGIN      
        1 ctgcctgcga agcgcggttt gaggcgctct tcgctgccct cgaactcagc gaggcggcga
       61 cgacaaacgt agttgcccaa gcccttcatc agggccacac gcggctttag gccgaacgcc
      121 cgcaaaagta gcggcaaatc cttccagaac agctgctcct gcagcgcacg cgtcgcggta
      181 gaaacgatga ctttgcgccc ggagagcagc gccggaagca ggtaagccat cgttttcccc
      241 gttccggtac cggcttcaca cagcacgatg cgctcgtcac gtagcgcacg ctcgacggcg
      301 cgcgccatct cgagttggct cgggcgtagc tcgtaacccg ggagtgtacg cgcgagcggc
      361 ccctctttcc cgagcaaacg ctcgagatcc tgcgggtcgg tcatcactcg cctgacgggg
      421 ccgggttatg tggcgaggag ggttcgacgt ccgtgtactt gaacccagca atcaagccgt
      481 cggattgctc ctggcaccag accaggcaag cgcgccgcgg actcgaagcg tctccgatcc
      541 aaacctcgta ttcgaggtcc ttctccagct ggcactccac gatggctcga aagccaccgc
      601 ggctcacgtt caaggtccag ccggtcgcct cgaagctcgc tgcccgtaac gtgatccgtt
      661 cggtagactc ggttcgcgcg ccccccgcgc tgcggcgtgg gacccaggga acagattcgg
      721 gcggagcaga ggcggggcgg ttcttcacct tttcaccgaa gcacctgaag cgtaccatca
      781 gctcgtcctc acgcacccac ccctgacgcg gtggccgcga atattgcgtc tatctagcgt
      841 tcgcactgtc cgtacgaagg cctaggtacc ggcgcctatc ctatgccgcc ggcaatggct
      901 gcaagggcat tggctgtaat atggcattgg ctgtaataag agtgcggcga actgctccaa
      961 tcaggtgagg gttaaaagcg cgccgcgcac caacccgcgc cgcgcaccaa cccagtagct
     1021 gtggagcgcg gcacgagtgt gcccaaatta accagcagcg tgagaagcca ctcgctggca
     1081 ataaacctat ggaaacaaaa gaacctcgat tgcttctgtc gagcggactg cccggagaaa
     1141 ccaggctgat ccctgggcgc ccggtgggtc gggggcggtg gaaggcgctt gacggtgact
     1201 agtcgcgatc gctaggctca cgggaagttt ggcgcttagt gccctgaccc cgcatccccg
     1261 cggggctacc gcaacctgga tccgctaccc agtgctccca aaagtgaacg tcgttgctgg
     1321 tttttggcga gtaccccctt cgagatgccg gctctgaact cgggtgagct caagaaagcg
     1381 cttcgagcga gcggtttcga aatttatcgg gcttcgccca gtgaagtgcg tttagcggag
     1441 cgagttcgcg aaaacctgct gatggatagc ggtgtcgccg tgcggctggc tgaaccgctg
     1501 agcgtcgccg tctatgtgcg cgccgagggc tccaagttct cgggtgaagc cgcggaacag
     1561 ctctatgcgc gagcgcgcga gcgcgcggag gcgctgcgcg tgcgaggctt cgaggaacac
     1621 gagtctcgcg cggtgccaat ttgggacccg ggggatcgga cgcaattgct cgatacttgg
     1681 tacgaggtgt gcttcgtgaa gccggtcgaa gcgagcgagc tcgaccaaac gcttcgcgat
     1741 gcgctctcgt tcgagaagac ggcctagctt cccgagctgc gctcgttgga acgcctgcgc
     1801 catggccatt cgcagagcta ttccattccg aattcccgta gacgtttgag gaacgtggga
     1861 taggaaacct cgagcgcttg tgcggcgacc atgcgtcgcc cgccgcagtg ctcgagtacg
     1921 cgagcgacgt acgcacgttc gacggcttcg agcgttggca ccacgccgtc cgctagcaga
     1981 tccaccgtaa aaaaagcgct gttgcgcggc gctccgaaac ggtgcggctc gatcaccagg
     2041 caactctcgt cgagctcggg gcccgcactc agaattactg cgcgctcgat gatgttgcga
     2101 agctcgcgca cgttgcccgg gaagtgataa cgcatcaacg ctttgagcgc gccatccgaa
     2161 atgccgctga tgcgtttgtt cactcgccgt gagaagaatt ccacgaaaga tttggcgagc
     2221 ggaggaatgt cctcgcgacg ttcggccagt ggaggcacgt tgacgatgaa taccgagagc
     2281 cgatgataca agtcttctcg gaagctgccg ccttcgaccc cggcgcgtaa gtcacggttg
     2341 gtggcggcca cgacgcgcag ctccaccgag agctcgcgct gtcccccgac gcgccgaaaa
     2401 cgcccggaat ccaagaactt caggagcttg gcctgcgatg cgtcgggcag ctccgttact
     2461 tcatccaaga acaacgtgcc gccgctcgcc atctcgatca ggccacgccg acgagtcttc
     2521 gcgtccgtaa atgctcctcg ttcgtgcccg aacagctcgc tctcgacgag ttgctcgggc
     2581 agcgccgcgc agttgacgtc cacgaacggc gcgtcccgat cggggtaggt gacccgatgc
     2641 agtagctccg ccgcaaattg cttccccgca cccgaaggtc cgagcaggag gacgggcgtc
     2701 tgagggctat gtcccagccg ttccagctcc gcgtgcacgc gccgcagcga cgcactttgc
     2761 ggcgctactt gcggcggttc gcggttcacc cctttcgcga gcgcgagccg atcgcgcaag
     2821 ccgcgcgttt cgagcacttt gtgaatcttg aagatcagat cggcgacgct ggtcggcttc
     2881 gttagatagt ccaccgctcc cgcgcgcatc gcggcgactg cattgtccac gtcgccatag
     2941 gccgtcacca cgattgtggc ggtggcggct gcgccgctgc tcttcagctc ttcgagcaga
     3001 tcgatgccat cgccgtccgg cagacgccga tccaaaacca tcacgtcggg ggccgccgtg
     3061 tccaactctt cgcgtgctcg gcgcaacgtg gggcacaccc gaacatcgaa gccctcgctg
     3121 gcgagagccg tctcggtcaa aatgcgaaag ctgcgatcgt cgtcgaccac caaaatgttg
     3181 ggtttcatga gacctcggcc ggcaaggtga gcaagaaggc ggcccctttc gcggagtctt
     3241 gcagaactag gtctccgccg tgggcctgag cgatgcgtcg agagagaaac aacccgagcc
     3301 ccgtcccgct gggcttcgta gtgctgaaag gttgaaacag cttggaccga acgggcacgg
     3361 gcacgcccgg tccattgtcc atgactcgta ggcgagccaa gcctggttca ctatcgagca
     3421 ccacgctgac ctcaccttcg cgcggggcgt ctgcgctcga gagcgcgtcg agtgcgttgc
     3481 cgatcaaatt accgaccact acgcgcagca gcgccggatc gaccttggcc atcagcgggg
     3541 cccgctcgac tttgacttcc aaccggcagc ccgcctcggc cgcgaggtcg agcgagagct
     3601 cactgaactt cacgactgac tctgccaagt ctaggtgcac cggatgcacc acgatgcctt
     3661 tcgagaacag catcaactcg gaggtaaagt cggccagacg ctgcatttcg tctcgcagct
     3721 gacgcacgat acgctcgtgt gcgcctccgc ctctcgcgtc gggcttcaag accaagtcca
     3781 acccgacgcg cagcccgttc agggcgttgc gcacctcgtg agcaatcaaa ttcgaagctt
     3841 cgccgagcaa ctccaggcgt tctcgctcga ccgcctcctg ctcactgcgc cgaaagatct
     3901 tcagcgctcg ccgcagcagt agaacgagca tcacgaacgg cgcgaccacg agcaccaggc
     3961 ccaccgaggc gcgcaagtac aagcggtgcc gagtcttggc gaacaacact gactcgggcg
     4021 tgagcagcgc gtaggatagt tcggtgccga ccaggggagc ggaggcgacg accatggcct
     4081 ctcctccgag ctccaggtgc atgctctcgg cgtgctcacg gggcttggtc agccaggtca
     4141 tcagcgcggg gttggtcgac agccctcggg catccgcggg gtacacgacc gcgccttcat
     4201 gagtgagcac gaatgcggtc gtgagcggag gggtgctgcg taaggttccg agcgatcggc
     4261 gactgagatc gatggcgccg agcaccacgc cgccgagccg ctcatcgtgc atggcgggtg
     4321 cgacgacgta gatcagcgcg tccttgcgct ccggcgccac gggtacgacc tggacttctt
     4381 cggacgcgac ggccttggaa aaccaagggg cgctgtgaaa agaagcattc tcgggcaaaa
     4441 agttgtgcgg ctcacaccag atcagattgc cgtcgcgatc caagatggcg acgccgacat
     4501 cgaacaacgt actcttcttg tgggcgagct cgagcagtct gcgctcggga gcgtcatcgt
     4561 ggtcggcgct ttcgatctcc gaacgcgacc cgaggcggac caactctgca ctgagcaggt
     4621 ccaggtagcg gcgcaatgca tggctgcggt cggtgaccgc ggtgcgcagg ttgccttcca
     4681 attgctcttc gctgtgcgtc ttctcggtga gcagagttgc gagcagcagc gtggcgacca
     4741 cgagcgcggt ggccgcgtat acggtcagcg tccattgcgc gagtcgtgca tgtgccgcgg
     4801 ccaacgtgac ggcacgaggg gctgcgccac cgggcgcggt cggcttggcg gatctagggg
     4861 tcactctcgt ccttctgtcg ctagggtgtg atgcgcgatt cgaagtgctt gcacgcggct
     4921 ttgatattct gactcaaccc gaaacttgga gctcaaacac atcctgacgg aaccgcgtga
     4981 agagtggaag ccgagtgcta cgaatgaggt cgtgcgacgc gcagctcttg gttggctgat
     5041 gggtcccgtc tgttgggtat tggcgtgttc ggaaagcgcg ggcccggcag gcggagtaac
     5101 gggtggcaat gccggcgctt ccaatgcagg agcgaatgca gcaggcggtg cgaatgccgg
     5161 cacttcggcg tccggtggcg gctccgccgc gggcggtacg gcccacgctg caggggcggg
     5221 gaacaacgcc ggtggcggaa gcaacgcggg cacaggaagc aacgcagcgg gctcgtcgaa
     5281 ctcgaacggc ggtcgcacca gcggggcagg cggcgcgccg gcgacgagtg gggcaggcgg
     5341 agtcggcggg cttgggggct cgcccgcagc cggtggcacc ttcaccgctg gaggaacttc
     5401 cagcggaggc agctcttcat ctcgaggcgg tgccacgaat tccggcggcg cggcgggtca
     5461 aggcggcagt accggtggcg cggcaggcgc agccagcaaa gggatcagca ttcagggcac
     5521 ccagctgatc gtcgacggca agccgtatca cgtccgaggt gtgtgctgga atccggtccc
     5581 caagggcaaa acccatcccc aaggcttgga tttcgcgggc gccgctgcga cggacatccc
     5641 gctcatgcaa cgcgccggca tcaacaccgt tcgcacctat gaaccgctga ccgacgtcgg
     5701 ggtgttggat caactctacg cggcggggat ccgagtgctc gacagtgtct acgtgtacgg
     5761 cggtgattca ccgagcgtag tcgctgaccg aatcacgaag atcaaagacc atcccgcgat
     5821 cttggcgtgg ctcgtcggca acgagtggaa ctacaacggt ctatacgtcg acctcccggc
     5881 ggcagacgcg caggcgcgcc tggtcgatgc tgccaagcgc attcgcgcca tcgatccgaa
     5941 tcatccggtg gccagtgttt acggggagct gccgtccaaa gccgtgatcg atgcaatgcc
     6001 ggagatcagc ctctggggca tcaattccta ccgcggcatc gacttcggag acttgttcga
     6061 taaatgggcg gcactcagca aaaagccgat gtttctcgcg gaatacggcg ccgattctta
     6121 caacgccaaa gctagcaagt acgacccgga gagtcaggcc acggccgttc gtgaactcac
     6181 gcaacaaatc gcggatcacg gttctgcctt ccactcaggg gccgtggcgg tcggcggcgc
     6241 cattttcgaa tgggcggacg agtggtggaa ggactcctcg ggtagcccga atgcgcaaga
     6301 ggttggcggc atagcgcccg gcggcgggcc ctatcctgat caaacattca acgaagagtg
     6361 gtggggcgtc gtggacatcg atcgcaaacc gcgccccgca tacgacgccc tgaagggcgt
     6421 tttcgactcg cttaccaaga caccctaaga gccatcccaa gaggagctaa tcgccggccg
     6481 ccgatagctc ttcgcgcagg ccctgcgcgg aggcgcgtgt tttgctggat tgccacgctc
     6541 gcagcggcac ggacgatcgc gcgcgcgtca cgacgagagc cagattggag cgcgagcgca
     6601 cccaaaaccc gccgagcgcc ggaaggtctc ccgccagcgc tgaaatccag gacgggcacg
     6661 cccgttgcac cacacgtcgg catgtccacc gaacgcatgc tttcgaagag tcccgctccc
     6721 ggcgagttgg ctctaccgag tgcaaaactc ggtgggcctt cgtccggtcc tgcccctcgt
     6781 gagtccgacg cggacgatgt ttggttcgtc ccttctgtga accaacgcgg agcgctgccg
     6841 gcgcgccccg ccgcggctga gttgccccag cccgaggagg agggagtgcc catgtccgtg
     6901 ctcgagcggc agagcacgcg cttaccgcgt ttggtcggta tcgcggcgct cgttctgtgc
     6961 ggctctggat tggctgcggt cgctatctgg gcggctgcca acaaggcccc ggacgtttcg
     7021 atccacgcgg caacgtcgtt ggaggcggtg gccgcgctca caccaccccg tggattgagc
     7081 gggcccgggg cgagcgagca cgcatctgca tcgaacaccg cgctgaccga agaccagagc
     7141 tccgccacca ggtcacgttt cctgcggcat gctccaagcg ccgagctctc gccggcgcgg
     7201 cttgcgggcg aacccgagat tgtaccggtg gacgagctcg tacccagccc agcggtggca
     7261 ccggaaagct cgagcctaca cgtgaacgtg gaccgccaga acgctgctgc tccgcagaga
     7321 gccgcactcc cgcacgtcac gccatccgca ccgagttacg gtgcgaaaac tcgcggtcta
     7381 cacctcaagt ccaagtccgt cttcgatcgc ccgatcgcgc cgcccgacgg attctattga
     7441 agcgcgccga gtcatcgaag cacacgaacg cgtgtggacg cgcgcccgcg tgaaggcgcg
     7501 attgagcgcc caccctctcc gcgcacggcg gcccgagtca cggcaagtag ccgctacttc
     7561 ttgtacgttg cctgtccgac gaacttcagc acccgctggc actcggcgct gccggtacgg
     7621 ccctcgcagc gatcttcgcc ctcgaagtgc gtcgccccag actgcagcgc gcgcaagatc
     7681 gcgcgggtca cgtcgtggcc gccttccttg cagaaggtgt cgtggcaagg cagctcgaac
     7741 aacgcgggcg cgtgcgcgac cgggatgcag cgcacatgcg ccgtctccgc cacgacggcc
     7801 cccgcgcgat gttcctgcag ctccaggcgc aggctgtcga gcgtgggcac ctcgtctttg
     7861 agacgcggcg cttcgtcttc gcggcggcgg cgttccgccg cgcgctccgc ggcctcgctg
     7921 tgtcgttgac gaaaacgatt catggatcct ctggggtggc ttgctggagc ggctcgggca
     7981 tcgactctgg agggggcggc aacaccgcgg cgacctgcgc cgtggcttct gctgcgccta
     8041 ggccaacacc ttgaacgctc gtagcttgaa cacgtgcggc cgctcctcct cgagcgcgcg
     8101 cacgtccggc cgcgcggggc ggcctctcag aattctcaag cgtgtcgaac cgagcatctt
     8161 cgcgctgttc gtcgcgatcc ggttcgctgc tcgccaacca cgctttcttc gcaacctcgt
     8221 aagcgcgccg cgtcagcgcc tccagttgct cgggaacgat gctgtaattt ggccctaacc
     8281 gacctgcggc acaggccacc acgcgagctc gcgcttcgct cagcgtgccg ggccaaccgc
     8341 cggccacggc tcgcccttct ttcttgaggg actgcgcgaa atcctgtgcc cacgcctctc
     8401 ccacgctttc cacggcgcgt tcgagtgcgt tgggggagcc ttgccttgat gcagtcatgc
     8461 ttacaaccga gagcccgggg ccatcttccg cacggcttcc ggccgtggtt cacgtggcgc
     8521 ggcggttgac ctgccgcgcc acgctcgttc acaaactcac cagcggttac gacgctccga
     8581 gccaccaccg ccacggaagc cgcgatcacc accacggtcg cccccgcgac cgccgccacc
     8641 gccgccgccg ccgaagccac cgccgccgcc gccaccaccg ccgaagccac cgccgcgggg
     8701 cttgcgagcc tctgcctcgt tcacgcggag ggcgcgaccg tcgagatcgg tcccgtccat
     8761 ctgcgagata gcgcgctgcg catcttccgc ggtcgccatc acgacgaacg cgaagccgcg
     8821 tgaacgaccc gtttcacggt cgagcacgag cttggcttca acaacgtcac cacattgagc
     8881 aaaggcccgc tgcagggtgt cctcagtggt gtgaaaagat agattgccta cgtacagtcg
     8941 gttgttcatg tctactctcg atgcggccgt tcaactggcg cacagggcac gcctgctggc
     9001 gtcgaggcgc cgcaaccctc gagtgccagc cgcagcgggt tatgaccgct gccgtcacag
     9061 gatcgctgcc ggagaaacga ctttccgcga tctctgaatc aagagagcag ccaggcggtt
     9121 accgaaaacg ctgtgaacga gcgcccgagg atagcaccgt c
//
LOCUS       DASTKU010000491         4394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_539712, whole genome shotgun sequence.
ACCESSION   DASTKU010000491 DASTKU010000000
VERSION     DASTKU010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539712"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VFQ61_16950"
     CDS             <1..933
                     /locus_tag="VFQ61_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956200.1"
                     /translation="AKGSAQATSSASGVILLLDTDDAIAVGDGLYASDPITVSGRQMP
                     RAEFVRCLRVAGRWTSTERGYTFEVRYPFLPLFALIRRVCRGEVNATELAFFRFFDAR
                     RWLSPRADPRATMESSLEQLSRDLPRFCEYLITSSETTRSALLHRFRAGASGWAELAT
                     RGPPDLARTLCDSYIGWLSNALPKMVDNYIWGSKRVDMICLEGFLFQTACGGGLRLSD
                     PVRRFLADRPWFVLLDLPQNQFYDSVPLSIFWFVSTRKASKSFPCLVVVAPTALNDPA
                     HLHNIERQLLSAGVAQRECTALKDWLRRSSRWNS"
     gene            complement(996..2645)
                     /locus_tag="VFQ61_16955"
     CDS             complement(996..2645)
                     /locus_tag="VFQ61_16955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HET9956201.1"
                     /translation="MTRRLARTGLMAAFVAGASLSAATSLAKPFEEYLRPTPITCSPL
                     SSASWGVAGVLPRDLCNGIESAKGARVHPDYYYWDGQIIRAKDGKYHLFMSTFSGNTN
                     FGTSWQSSDAYHAISQTSVFGPYERQDYIYTNNGSHKGHNVSAVELPDGSYAVIVSEI
                     VPFTIYKSSSLDGPWTSCQPTTQVGASNISLVPRHDGKFQIVERNGTIAIADTLCGTY
                     VKQKPTCSYTQAGADSIYPKRTSIPGVSNPSFMWQEDPHIWRSAGTYHVIYSGSGDRV
                     GWHVYSADGINWKDNGYAWSPRDYQKIFCYEGTTTCSQWYKMERPGVVLQNGHPTHIT
                     WAVSDVDKDNQVLPGTNHGTKIVVVPFDGVAFDNDFGVGGGANGGASNTGGAPSTGGA
                     RASGGSSGAGGAKATGGSSGAGGAKATGGSGGTSGSSSNTGGVAHGGGPTGASGGATS
                     MPGTGGQPSTSSSGGSANVASGGDRALGGTSASGGTNGNGGTPAAGSSNNPGNPADSG
                     DNGGCGCRTAGGARSNALAALGIVALVGLRARRRRAKSRCT"
     gene            complement(2853..3413)
                     /locus_tag="VFQ61_16960"
     CDS             complement(2853..3413)
                     /locus_tag="VFQ61_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9956202.1"
                     /translation="MGLLTFSLNVTLDGCVDHQEGIVEDETHAFFTRMLDESGAMLWG
                     RVTYEMMESYWPAVARGDAPAPPALREWAVKLEAKPKYVVSSTRKDFPWTNSHHITGD
                     LRTGVQRLKDATSAGVLVGSGKLATELDRLDLIDEYRFLVHPRIAGHGPTLYQSGLPS
                     TRRLDLVSATPLRSGPVALHYRRALT"
     gene            complement(3401..3640)
                     /locus_tag="VFQ61_16965"
     CDS             complement(3401..3640)
                     /locus_tag="VFQ61_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956203.1"
                     /translation="MRVAASADLIDDREYAAPPELGGVPTARGTRMTVAQRVAEPELA
                     DSAAVVRHRRLAAVGWGQTAQRLVTYLERSTSWDS"
     gene            complement(3618..3962)
                     /locus_tag="VFQ61_16970"
     CDS             complement(3618..3962)
                     /locus_tag="VFQ61_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9956204.1"
                     /translation="MSQTFAALADPTRRAILKRLAAGPANVKELSQPFDMSGPAVCKH
                     LRVLERAGLVRRRREAQSRPCELDPAPLKAIAAWTEAYREFWDASFDRLGVYLRQLQA
                     PSRRPHARRRKR"
     gene            complement(4164..>4394)
                     /locus_tag="VFQ61_16975"
     CDS             complement(4164..>4394)
                     /locus_tag="VFQ61_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956205.1"
                     /translation="NPFTDSDARDGTWVISAYNAQTNQFEIFASAAGSAKRKLGCIQS
                     QNTTVHAIGVGKGAAYISILRDEKASILKLPL"
BASE COUNT          697 a         1376 c         1447 g          874 t
ORIGIN      
        1 gcgaagggat cggcgcaggc gacgtcgtcc gcgagcggag tcatcctgtt gctcgacacg
       61 gatgatgcga tcgccgtcgg cgacgggctc tacgcgagtg acccgataac cgtgagcggc
      121 agacagatgc cccgggctga gttcgtgcgg tgcctgcgtg tcgcgggccg ttggacgagc
      181 accgagaggg gctacacgtt cgaagtacgg tacccgttcc tgcccctatt tgcgctgatt
      241 cggcgggttt gccgcggcga ggtgaacgcg accgagctcg cgttctttcg cttcttcgac
      301 gcgcgccgtt ggctatcgcc gcgggcggat ccgcgcgcca cgatggagtc atccctcgag
      361 cagttgtcgc gggacttgcc acgtttttgc gagtatctaa tcacctcttc agagactact
      421 cgcagcgcgt tgctgcacag gttccgtgcg ggcgcttcag gatgggctga gttggcgacg
      481 cgcggtccac ccgacctcgc gcgcaccctt tgtgattcgt acattggttg gctcagcaac
      541 gcgctcccca agatggtcga taactacatc tggggctcga agcgcgtcga catgatttgc
      601 ctcgagggat ttctgttcca aacggcgtgc ggagggggac tgcgtctgtc ggatccggtc
      661 cggcgcttcc tcgcagacag accgtggttc gtgctcttgg acctccccca aaaccaattc
      721 tacgattcgg ttcctctctc gatcttctgg tttgtgtcca ctcggaaggc ctccaaatcg
      781 ttcccgtgct tggtcgtggt cgcgccgacc gccctgaacg acccagcgca cctgcacaac
      841 atcgaacggc aactcctgag cgcaggggtc gcgcaacgtg aatgtaccgc cctcaaggac
      901 tggctccgcc gcagcagccg ctggaactcc tgaggtgaag cgtggccctc gttcggttct
      961 ttcttctgca ctcgcgtgcc tgcccctcgg tgactctagg tgcagcgcga cttcgctcgg
     1021 cgccggcgag cgcgcagacc gacgagcgct acgattccga gagccgccaa ggcgttgctg
     1081 cgagcgccac ctgccgtacg gcagccgcag cctccgttat cgcctgagtc agcggggttg
     1141 cccgggttat tggacgagcc cgcggcaggc gtccctccgt taccgttggt cccacccgac
     1201 gctgacgtgc cacccaacgc acggtcgcct ccgctcgcta catttgccga gccaccggag
     1261 ctactcgtag acggttggcc tccggtccct ggcatcgaag tcgcaccgcc ggaagcgccg
     1321 gtcgggccac cgccgtgcgc gactcccccc gtgttggagc tgctcccgct ggttccgccg
     1381 cttcctcccg tggctttggc accgcctgcg ccgctgcttc ctcccgtggc tttggcgccg
     1441 cctgcgccgc tgcttccgcc gcttgcgcgg gctccgcccg tcgaaggagc gccacctgtg
     1501 ttcgaagcac cgccgttggc gccgccgccg accccgaagt cgttatcgaa agctacgccg
     1561 tcgaagggca ccacgacgat cttcgtgccg tgattggtgc cgggcaggac ctggttgtcc
     1621 ttgtcgacgt cggatacggc ccacgtgatg tgagtagggt ggccattctg cagaaccacg
     1681 ccgggccgct ccatcttgta ccattgcgag caggtcgtgg ttccttcgta acagaagatc
     1741 ttttggtaat cgcgcggaga ccaagcgtag ccgttgtcct tccaattgat gccgtcggcc
     1801 gagtacacgt gccagccgac gcgatctcca gaacccgaat agatgacgtg gtacgtgcct
     1861 gcgctgcgcc agatgtgggg atcctcctgc cacatgaacg aaggattgga tacgccgggt
     1921 atcgaggtcc gcttcgggta aatgctgtcg gccccggcct gggtatagga gcaggtcggc
     1981 ttctgtttga cgtaggtgcc gcacagggtg tcagcaatgg cgatggtccc gttgcgctcg
     2041 acgatttgga atttgccgtc gtggcggggg actaggctga tgttggacgc accgacctgg
     2101 gtcgttggtt ggcaactcgt ccatgggccg tcgagagagc tcgatttgta gatcgtgaag
     2161 ggtacgatct cgctcacgat gacggcatag ctgccgtccg gcagctcgac cgcagaaacg
     2221 ttgtggcctt tgtgcgagcc gttgttcgta tatatgtagt cttgacgttc gtaagggccg
     2281 aacacgctgg tttggctgat ggcatggtaa gcgtcggagc tttgccagct cgtgccgaag
     2341 ttggtgttgc ccgagaacgt gctcatgaac aggtggtact tgccgtcctt ggctcggatg
     2401 atctgtccgt cccaatagta gtagtcgggg tggactctcg cccccttggc gctctcgatg
     2461 ccgttgcaga ggtcacgcgg aagcacgccg gcaacgcccc acgacgcgga gctcagcggt
     2521 gaacaggtga taggggtcgg tcgcaggtac tcctcgaacg gcttggcgag ggaggttgct
     2581 gcgctcaggc tggcgcccgc aacgaaggcg gccatgaggc ccgtgcgcgc gagccgtctt
     2641 gtcacgaagt ccccctggcc gactgtgggc ggatggcgtt cagaccggga cctggtcgaa
     2701 aggaacgggg ctcgcgcttt ggtcacactg gttactggcg gaacaggcca gaatgtgtcg
     2761 aaaattcgtt tcagaggccg cagagcgctg cgcgacgggc cctaacaggg cgttgacaaa
     2821 cgccgtgacg cagcgtgttt ttcaacgccc tgctaagtca gcgcgcgccg gtagtgcagg
     2881 gcgaccgggc cgctacggag cggcgtcgcc gagacgaggt cgagccgtcg cgtgctgggc
     2941 agtccgctct gatacagcgt cgggccgtgg ccggcgattc gggggtggac gaggaacctg
     3001 tactcgtcga tcagatccag ccggtcgagc tcggtcgcga gcttgccgct accaacgagc
     3061 acgccagcgg aggtagcgtc cttgagcctc tgcacgcccg tgcgcaggtc cccggtgatg
     3121 tggtggctgt tggtccacgg gaagtccttt cgggtcgacg acactacgta ctttggcttg
     3181 gcctcgagtt tgactgccca ctcgcgcaat gcgggcgggg caggtgcgtc cccgcgagcg
     3241 accgccggcc agtagctctc catcatctcg taagtgacgc ggccccatag catcgccccg
     3301 ctctcgtcca acatgcgggt gaagaaggcg tgggtttcgt cttcgacaat tccttcctgg
     3361 tggtcgacgc agccgtccag ggtgacgttt aggctgaagg tcaagagtcc catgatgtgc
     3421 tccgttcgag ataggtgacg agacgctgtg ccgtttggcc ccaaccgacc gctgcgagtc
     3481 tacgatgacg tacgaccgct gccgaatcgg cgagctccgg ttcggcgacc cgctgcgcga
     3541 cggtcattcg cgtcccgcgc gcggttggga caccccctag ctccggcggt gccgcgtact
     3601 cgcgatcgtc gatgaggtca gcgcttgcgg cgacgcgcat ggggcctccg agagggtgct
     3661 tggagctgtc ttagatacac acccagccga tcgaagctcg cgtcccagaa ctcgcggtaa
     3721 gcttcggtcc atgcagcgat cgccttgagc ggcgcgggat cgagctcgca cgggcgcgac
     3781 tgcgcttctc gacggcgccg gacgagaccg gcccgctcga gcacgcgcag gtgcttgcac
     3841 accgccggcc cgctcatgtc gaacggttgc gacagctcct tcacgttcgc ggggcccgcc
     3901 gccagccgct tcaagatcgc acggcgggtc ggatccgcga gcgcggcgaa cgtttggctc
     3961 aaggcgtccg cttgcatcta accagatggt tacataaccg aatggttaga tgcaaggcga
     4021 aataacgacg aacggccgca gagggcccag ttcacgtggt agtgacgaag cgcgaggtcg
     4081 ctcgtacccg gcgccaatcg acgctgcgca ccgacgctcg ccccctccgt ccgagttagg
     4141 gtgggggcca actgcgtggt cgctcagagc ggcagtttca ggatgctcgc cttttcgtcg
     4201 cgtaggatcg agatgtaggc ggcacccttg ccgacgccga tggcgtgtac ggtcgtgttc
     4261 tgactctgaa tgcaaccgag cttgcgcttg gcgctgcccg cggcagaagc gaagatttcg
     4321 aattggttcg tctgcgcgtt gtatgcgctg atgacccagg tgccgtcgcg ggcgtcgctg
     4381 tcggtgaacg gatt
//
LOCUS       DASTKU010000492         4840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1654974, whole genome shotgun sequence.
ACCESSION   DASTKU010000492 DASTKU010000000
VERSION     DASTKU010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654974"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..1386)
                     /locus_tag="VFQ61_16980"
     CDS             complement(268..1386)
                     /locus_tag="VFQ61_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956206.1"
                     /translation="MEELASNILNLCSHLARWKGHEGQLLKLLADGPTAEASVHAVDA
                     PAPEDAVQPSSGIEVAAAETPQHFVESEAPTAASAVDQPNAMTDQPGEPGTETAEVSH
                     TEQDASPSPHAQEEPAADAPLAETTGEEPAPAHVDAASPLDETPPVAEPTVADAPAAQ
                     DQHETALGEASSDEAMTEAAAAEPSRAEAEAVSEEGSAHTSSDASTASITPEHEASPM
                     EEKASETVAETAPDDLPTAASSEPEGTPPAPEAAPVAEAEAVVVAEAVAEVTPSEDAG
                     TAPVGDVAEPVAEAEAVASAEPPAPEAANAVAEAEAAPVEPAPTEAIAAENLPMHVPT
                     EAQTEAEAPVDTHSEHEREVPEKSGVNSPIPEAMEHAG"
     gene            2554..2952
                     /locus_tag="VFQ61_16985"
     CDS             2554..2952
                     /locus_tag="VFQ61_16985"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HET9956207.1"
                     /translation="MTEPTRSLFEQMGGEPALRLVIDEFVDRMFDDVMIGYFFARVSR
                     EHIKRMEYEFAAQKLGADVLYSGRSLEVAHRAHRIFDGQFLRRLTLLRETLERHGVPE
                     SVKEFWLRLTESERDTVVAAFCESPAVTRE"
     gene            2964..3845
                     /locus_tag="VFQ61_16990"
     CDS             2964..3845
                     /locus_tag="VFQ61_16990"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HET9956208.1"
                     /translation="MSGAVKSVARVGGLSTGLLACLVLLASCNTAGSAWMSQPLGPAD
                     SDAMRVEDANWQTGASAPPSRRHPSSRRLSLVDSSSLGSGPGGVERPAVEGEGAPTLG
                     RSLGQFRNTYYDFPNEADFDGGPVPLMNGACQLIREVPRSFFESVCVQGSGTLRVGKT
                     VSFARRDCECAEVCPRTQQKICFDVLDANAFPWGRGSTGKPIVPFVTVAVDPEVIPLG
                     TPIYVPEYDGVPRDPSAHSTHDGCFIAQDRGIRVKGKHIDVFTGDPAMTSIWNQQVPS
                     NRGVAVYVDSPRCRRGP"
     gene            complement(3849..>4840)
                     /locus_tag="VFQ61_16995"
     CDS             complement(3849..>4840)
                     /locus_tag="VFQ61_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956209.1"
                     /translation="RAVTLAVEAGTRVGKRDMAVTHATRMAFFEQVRAGYPNDCHDGF
                     FQGPAPASPTLLPVRTRGRRQVHDAVWASSYQPFWTAFADRYRTVPQSPNVTARLFST
                     GEPRPIAVLLHGYMSGHFAVEERIWPLNELDELGFDTALFVLPFHGPRAAPGRSLQPE
                     FPSGDPRMTIEGFRQAVTDLRAFVRFLRERGHPRVGLLGMSLGGYVAALTATLEPELD
                     FLVPIIPLASLSDFAREQGSLSAEPAARAREHTALDRLYRVVSPLGRPPLITGDRVLV
                     LGARADRVTPIEHARALSAHFQARLLAWPGGHLLQIGRKRSFESVYALLDRVR"
BASE COUNT          787 a         1504 c         1537 g         1012 t
ORIGIN      
        1 ggccgagcgt ctcgggttgc ccgaagctcg ccgccgcctc gccaaactcg ataaggagtg
       61 agacgagcgg cggcggcttg ggggcggcaa ctcgaggacg gagggcagcg caggaggcta
      121 gccaataggc aactcacgct cgagccgaga gtcagcagcc gtggttcggc aaccgcgctc
      181 ccgagtcgtg cctggctaat cgaatcctgc gtggctaatc gtgcttgcct aactcggtgc
      241 gaacgggatg gcgggcaggc ttgcagttca acctgcgtgt tccatggctt ccgggatcgg
      301 gctgttcacg ccggacttct ccgggacttc tcgctcgtgt tcgctgtgcg tgtctactgg
      361 tgcctctgct tcggtctgcg cctcggtcgg cacgtgcatc ggcaagttct ccgccgcgat
      421 ggcttcggtg ggggccggct ccactggcgc cgcttctgct tcggcaacag cgttagcggc
      481 ttccggtgcc ggaggttctg cactggcaac ggcctccgct tccgcaacag gctctgctac
      541 gtctccaacg ggtgctgttc ctgcatcttc agaaggtgtt acttcggcta cggcttctgc
      601 cacgacgacc gcctccgctt ctgcaacggg cgctgcttct ggggcagggg gtgttccctc
      661 cggttcagac gacgccgcgg taggcagatc atcgggtgcc gtctccgcca cggtctcgct
      721 tgccttttcc tccatggggc tcgcttcgtg ttccggagtg atgcttgcag tggatgcatc
      781 actggaagtg tgcgcggagc cttcttctga caccgcttct gcttctgctc gggagggttc
      841 ggcggctgcg gcctcggtca tcgcttcatc gctagaggcc tcacccagcg ctgtttcatg
      901 ctgatcctgg gctgctggag catccgccac ggtgggctcc gcgactggtg gtgtttcatc
      961 gagcggggac gccgcgtcga cgtgggccgg ggcaggttct tcgccggtgg tctcggctag
     1021 cggtgcgtcg gcagcgggct cttcctgcgc atgagggctc ggggaggcgt cttgttcagt
     1081 atgcgatact tcggcggttt cggtcccagg ctcaccgggc tggtccgtca ttgcgttggg
     1141 ctgatctacc gcggaggcgg cggtgggtgc ctcggactcc acgaagtgtt gtggcgtctc
     1201 ggcagccgcg acttcgatgc cgcttgacgg ctgtacggcg tcttccggag cgggcgcgtc
     1261 tactgcatgg acgctcgcct ccgcagttgg cccatctgcg agcagcttga ggagctgccc
     1321 ttcatgccct ttccagcgcg cgagatgtga acacaaattc agaatgtttg atgccagttc
     1381 ttccattgtt gccgctccta atttccgctt acctgctctt gaaacgcccg catcgccgat
     1441 acgggggtgc ctattggacc tggatttgcg cctcttaatc cggtagcggg caatcgcccg
     1501 cgctctcgga cgcatcttgg ctgcaaacgt gatcgccatt gccctgcggc tgcgcgcgcc
     1561 gaccgcccac tgagagcgcc tgcgaacagg gcgcctgcag agcgggccgt atgccgggtt
     1621 agttctgtgg cgtacccttc aggagtggag tgctgattac cgcccctgaa gggcttcatg
     1681 ctcgattgct gggcgccgaa cgcccactac tcagggctcg ggcttgcttc ggagcttgcg
     1741 cccgtttcgg ggcgctcgcg ccggagagct tgcgctttat ctgacgagtg ttgtctagcg
     1801 cgaggacgca cctcgactga ctcgcttact gcgtgacgcc gaggtggctc ggctgctttt
     1861 cggtgtcgag tcacctcgac gcccgcccac ttggttgctt cggtgctgct ccagagacgg
     1921 tagcagcgca attcaggatt ggaactatga ctctgcgaag tgtttttcgc aagcgcgaag
     1981 ctcgagagaa cgcgagtttt ccgacgtgac actctcactc tctgaactgg agagactctc
     2041 gaaggtctca tcgaaagaga cgccgttggg atctgttccg aaccgtctgt tccgaaccgt
     2101 ctgttccgag ctcttctttc agagcccttt gtgcttctga cggggatcgc gcagctccgc
     2161 gacgctatca ctccgttcgc gtacgttgct cggggttgcg aacccaagct tggtggagcc
     2221 gctgtgatgc ccgactacgc accgaagctc gatgcgcccg ttccccgccg caggcgacac
     2281 ccctttgcag cctagtttgg attgctatga tccgcccagc gcgggcctat gagcacgggg
     2341 taaacatgta ctttgccggc gaagagtcgg gattgactgg caaggcgggt gagagcggaa
     2401 gcgatggggg cccggtgcga agctccaccg tttctgctgc cttagccgct gtctcttcat
     2461 cgctgtctct tcgtcgctgt attttttgcc gctgtttctt gcgggtcgcg cgtgcgaagt
     2521 gcccgtctgc ccggtacaga gtaaggttcg gtggtgactg aacctacgcg ctccttgttc
     2581 gaacagatgg ggggtgagcc tgccctgcgc ctcgtgatcg acgagttcgt cgatcggatg
     2641 ttcgatgacg tgatgatagg ttacttcttt gcgcgcgtgt cacgcgaaca catcaaacgc
     2701 atggagtacg agtttgcggc tcaaaaactc ggagccgacg ttctttattc cgggcgttct
     2761 ttggaagtcg cacaccgtgc ccaccgcatc ttcgatggtc agtttctgag gagattgacg
     2821 ctactgcgcg agacgctcga gcgacacggc gtccccgaat ccgtaaaaga gttctggcta
     2881 cgccttacgg agtccgagcg cgacacagtc gtagctgcat tttgcgaatc ccctgcagtg
     2941 acgagagagt aaggaaacct tcgatgtccg gtgccgtgaa gagcgtcgct cgcgtcggcg
     3001 ggttgtccac aggcctactc gcctgcttgg tactactggc ttcctgcaac acggcaggat
     3061 ccgcgtggat gtcgcagccg ctcgggccag cggactcgga cgcgatgcgc gttgaagatg
     3121 ctaactggca aaccggcgca tccgcgcccc cctcacgtcg gcatccgtcc tcacgtcggc
     3181 tttcgctcgt cgactcctcg tccctcggca gtggccccgg cggggtggaa cgacctgcgg
     3241 tggagggtga gggggcgccc acgttgggtc gtagcttggg tcaatttcga aacacgtatt
     3301 acgacttccc gaacgaggct gacttcgacg gcgggccggt ccctttgatg aacggcgctt
     3361 gccagctgat tcgagaggtg ccccgcagct tcttcgaaag tgtgtgtgtg caggggagcg
     3421 gcacgctccg agtcgggaaa acggttagct tcgctcgccg ggattgtgaa tgcgccgaag
     3481 tctgcccccg cacccagcag aagatctgtt tcgacgtttt ggacgccaac gcctttcctt
     3541 ggggtcgtgg ctccaccggc aagcccatcg ttccgttcgt gaccgttgca gtggacccag
     3601 aggtgattcc cctgggaaca ccgatttacg tccctgagta cgatggagtt cctcgcgatc
     3661 cttcggcgca cagtacccac gacggctgct tcatcgcaca ggatcgcgga atccgtgtga
     3721 agggcaaaca catcgacgtc ttcacagggg atcccgcgat gacgtccatc tggaaccagc
     3781 aagtgccaag caatcgcggc gttgcggttt acgtcgacag cccgcgttgt cgtcggggcc
     3841 cttgagcttc agcgcacccg atccaatagt gcgtagacac tctcgaagct gcgcttgcgt
     3901 ccaatctgca gcagatggcc accgggccac gccagcaatc gtgcctgaaa atgcgctgac
     3961 aacgctcgcg cgtgctcgat cggggtcacc cgatcggctc gagccccgag caccaaaact
     4021 ctgtccccgg taatcagggg tggtcgcccg agcgggctca ccacccggta gagtcgatcc
     4081 aacgccgtgt gctcccgcgc ccgtgccgcg ggctccgcgc tcaaggaacc ctgttctcgc
     4141 gcaaagtccg acaacgaagc caacggaata ataggcacga gaaaatcgag ctcgggttcg
     4201 agcgtagccg tgagggcggc gacataaccg cccaggctca tcccgaggag gccgaccctc
     4261 gggtgccccc gctcgcgcag gaagcgaacg aaagcgcgaa gatccgtcac ggcctggcga
     4321 aacccctcga tcgtcatacg cgggtccccg ctcggaaact cgggttgcag gctgcgcccc
     4381 ggcgcggcgc gcgggccgtg aaaaggcaga acgaacagag ccgtatcgaa gcccagctcg
     4441 tccagctcgt tcaaaggcca gatgcgctct tcgaccgcga agtggccgga catgtaaccg
     4501 tgcaacaaca ccgctatcgg gcgaggctct cctgtcgaga agaggcgcgc cgtgacattc
     4561 ggactttgcg ggaccgttcg gtaacgatcc gcgaacgcgg tccaaaaggg ttggtagctg
     4621 ctcgcccaca cggcatcatg gacttgacgc cgccctcgcg ttcgcaccgg tagcagcgtg
     4681 ggggaggccg gagccggtcc ttggaagaag ccgtcgtggc aatcgttcgg gtagcccgcc
     4741 cgaacctgct cgaagaacgc catgcgagtg gcatgtgtga ccgccatgtc gcgcttcccc
     4801 acgcgagttc cggcttccac cgcgagagtg accgctcggt
//
LOCUS       DASTKU010000493        22120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1433080, whole genome shotgun sequence.
ACCESSION   DASTKU010000493 DASTKU010000000
VERSION     DASTKU010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 22120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22120
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433080"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2178)
                     /locus_tag="VFQ61_17000"
     CDS             complement(<1..2178)
                     /locus_tag="VFQ61_17000"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9956210.1"
                     /translation="MQWLANVSVRRPVFATVLILVILVLGVVGYLQLGVDRFPKVDFP
                     TVAVVTRLPGSASSEVETDVTDKIEEAVNTISGIDELTSVSSEGVSQVYVTFVLEKDI
                     DVAAQEVRERVNGVLQNLPEGIEAPSVLKMDPDATPVLYIAVNANAPTREITEVADKI
                     VRRRIENARSVGQVTIVGGTKRQVNVSLDPSRLRAYGITAVDVERAIRAANLTLPGGR
                     IEAGPSQQVLRVRGRVEDASEVRLLSILERDGSVVRLGDVAEVEDGIETPETVAVRDG
                     KPAVVLAVRKQSGSNSVAVVDAVRERMSDLSRELPKGYSLEVVRDNTSTIRTSVAAVK
                     EHLLLGGLFAGAVVLLFLGSVRSTLIAAFAIPVSIIGTFALMWIQGFSLDTITLLALA
                     LAVGIVIDDAIVVLENIHRYIEEKGYSPTKASILATREIGLAVLATTLSLAAVFLPVA
                     FMSGIVGRFLRSFGLTMAFSILVSLIVSFTLTPMLSSRWLRAHRPVGATGEPRKVLLE
                     RIVDRFYLPMERAYMAVLAWVMRRRYTVVLASLASLIACGPLLARVPKGFLPKSDEAH
                     FEVNLRAKEGTSIDATALLGERLARELRTMPEVTSTLVTVGDNNDRRPNLARVYVRLT
                     DPEKRKLSQDELMDRVRKQILPHQPKDLTLDVSQVAMFSGGGFRTALIQYQISGPDLD
                     ALAKYTDQLVERVRKVPGAVDVASTYVGGKPELALRIDRERAAE"
     gene            complement(2302..3414)
                     /locus_tag="VFQ61_17005"
     CDS             complement(2302..3414)
                     /locus_tag="VFQ61_17005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9956211.1"
                     /translation="MTFMARANHLRANHLSVFAVFGTLLSFTAVACTRAEAAEKAVAK
                     PVAAQKIAVKTVEVSRKSLPKTLTITGTLIANRESEVAADIAGRVVESAIERGQRVEK
                     GALLARLDSRSAVLSRAVASAELETARVHRDNASLECERAEQLFASSALSRAELDRTR
                     ANCRASDTSTQAALARTRVSDKAIADAFIRAPFAGVVTDRAVDVGEYVTPGRRIATLV
                     EINPLRLELSIPESATAAVQQGRDVQFSVRALPEKRFRASVRYVGPVLRRSTHDLVIE
                     AVVDNTDGALRPGMFAEAELNTGEQELSVVPKESVRGAGAAARVFVVKDGRVEERVVL
                     AAEPRGNDVPVISGLDVGERVVTSPSAEVVDGALTL"
     gene            3684..5087
                     /locus_tag="VFQ61_17010"
     CDS             3684..5087
                     /locus_tag="VFQ61_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9956212.1"
                     /translation="MQKVLVVDDQEAVRTALQVLLDVHDIPCVTASTPEAALSLVRTE
                     DLGAVIQDMNFGAGTTSGQEGLRLFREIHALDPELPVILITAWTSLETAVELIKEGAS
                     DYVAKPWDDEKLVASIQNLLRLRALKLENTRLWAQEERLRGELGQRFDLCGLVYRSRA
                     MHEAVSLAVHVAPSEVPVLITGPNGSGKEKLAEILQANSRRKREPFVKVNAGALPDQL
                     LEAELFGAEAGAFTGAIKQRVGRFEAAHGGTLFLDEIGNLSASGQMKLLRVLQTGEYQ
                     RLGSSHTRNVDVRVIRATNADLRQEIAHGRFREDLYFRLNVIEIHVPPLAERCDDIVP
                     LAEHFLALHTVPGRERAELSDEAKFALEQHDWSGNVRELENRIQRALLVSGGERVTPS
                     DLGLESNLTGPGRPSSPPRPANSPDSEERRAVEAALVRARGVVSRAAADLGVSRQALY
                     RRMERLGIELERRPKTP"
     gene            5137..6885
                     /locus_tag="VFQ61_17015"
     CDS             5137..6885
                     /locus_tag="VFQ61_17015"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9956213.1"
                     /translation="MTAIAKSTVEKADTISHTLESRAVPELAQDRPSGERTRSRIQRR
                     TITLALPSGMTPLDSEEGLRFLSGRLAMFSVVVCALGVFALLATQLLKLGLPDYEFPR
                     TFLTEGGLQHSVAFGISLAIFAIARNYKYGLLGLLTLDAAGTLLSLSAYAAMCLSNMR
                     MPQRTDITMMLITLSVLTLRAVVIPSSWAHTLCVGVLASLPALVLTYLNAPRVAALGL
                     PFPTWIALGYMGVWTVLGLSISTLASHVIYGLRCQIAQVRQLGQYVLESKIGEGGMGV
                     VYKARHSLLRRPTAIKVVLPERVGEQVLRRFEREVQLTARLTHPNTVSIYDYGKSPDG
                     NFYYAMEYLDGVDLEQLVSRFGPLPTARVIHVLKQVAASLAEAHGVGLVHRDIKPANI
                     ILCERGGNADVAKVVDFGLVKDVQGLGRNKSASLSTVHAVVGTPLYMAPESLVNPERV
                     DARADLYALGAVGYYLLTGLPLFQGASLVEVAAKHLHERPVAPSERTTLPIARDLESI
                     VLRCLEKDSAKRPASAQELLSQLEACESDTWTADLAHEWWNENGERARAGRQQELADD
                     TNHQHATLCVDFAARC"
     gene            7047..7814
                     /locus_tag="VFQ61_17020"
     CDS             7047..7814
                     /locus_tag="VFQ61_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956214.1"
                     /translation="MFRKAMPSKALSWVSSLSIAFFAAHCTVTSDDDDTTACEPNETR
                     TCSCSDGSKGTQTCTPGGTRFGACAPCTGSTGGAGGENSAGGESGSTSGGTTSGGTTH
                     TGGSGGSTTQAGSGGDTGGVTSSGGAGGNGGAGGDANVGGAGALCADAVPVDKCDECL
                     LSQCCEEYVACSGEAECSDVWSDFAKCVEDLRVNGEATPANVKQCADIINVDEDENPL
                     PPSPATGALIDCMGGSDSWSSASSWPSTACTTGCFKP"
     gene            7900..8511
                     /locus_tag="VFQ61_17025"
     CDS             7900..8511
                     /locus_tag="VFQ61_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956215.1"
                     /translation="MNKYRLASIALLALCIGSGTAAADDAQTERQKRFEELRKKREEQ
                     RKAASAGKGGSNAGGATSTGSAAKSGGATGKGGSSHSGGSGGSASGGGAGKGGATAAP
                     SPDSQLDELRKTRTARRQSVQRELRERWGSALDNPRSRDELKTHGRRVAELARLRSLC
                     EQQKRVADLATVDKLLTREELRHASAMNDIREGKPAAAPGGTP"
     gene            8508..8855
                     /locus_tag="VFQ61_17030"
     CDS             8508..8855
                     /locus_tag="VFQ61_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956216.1"
                     /translation="MSTRSRFGWQLSCATLVAIAFASSGCRGLLKKRAPVEGASATPS
                     TTTAPLASAPLPTAPSPTAPLPSVPPAATAAAVTQDADLPTPQDFEEDAFEKITAKNL
                     QAELTRLSKEIGN"
     gene            complement(8956..9561)
                     /locus_tag="VFQ61_17035"
     CDS             complement(8956..9561)
                     /locus_tag="VFQ61_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956217.1"
                     /translation="MKRSQFIFAACTLWCFGQIGTAHAQSTIRHPGQRPHYDFELEPH
                     LLLGAFDPPGPAHGGDGLGLGFRGTIEVAPEGFISKLNDSVGVGFGMDWVHYSTSAAR
                     GRCSRFIDGPNGTLICAEVDTQYSRDYFYVPIVMQWNFWLARRWSVFGEPGLSLNFPR
                     HRDMSISPALYAGGRFHFSDQVTLTMRLGYPTFSLGVSFLL"
     gene            complement(9636..10325)
                     /locus_tag="VFQ61_17040"
     CDS             complement(9636..10325)
                     /locus_tag="VFQ61_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956218.1"
                     /translation="MTPCPTNHPTLDPAADRALDSPLAHQRSSTTSKTMLSRASRSAP
                     ARTKRALITKGPLWWFALALVSAALATACDSGGNCDEGDFCDCAGGQECYLACSGDAC
                     RQSCHDMDRCGSVCEDNCDAACYGTHECSAYCSQNCNIDCHDTDRCGAACGDGCSYRC
                     TRANACGVRVGRNSRVTCADVSSCVVECAGFCLVTCQRVGGRGCEVTCADGSGAVDCG
                     NGVLACGPCSN"
     gene            10838..12937
                     /locus_tag="VFQ61_17045"
     CDS             10838..12937
                     /locus_tag="VFQ61_17045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET9956219.1"
                     /translation="MTDTHSDPKVRSYAVAAPPVSRGARPTRSSSPPLGTTDRESSAL
                     KRRFWTAVAGFVPIAFLELGAGAISSSLRAWLECLLACPAVFYSAAPFFVDAGRSVRR
                     ARPDMFALIALGVAAAFGFSVLGLLMPTLLPAAARGTYYFEAATGIVALVLFGQLLEL
                     SARRGTRRHLEALQNLLPNSARRVAPDGSEQRVPCTQLLPHDLVRICPGERLAADGLI
                     VEGEGQLDESLLTLEKAPVKKGPKQPVHAGTLNTGGTFTMRAERLGSNTLLSRITLAV
                     SQAQSARGSLQREADRMAAWLIPSVIALALITLGWWLGWAPGVAPDARLAHALVSAIA
                     VLVVACPGALALAAPLSMSIAAGRGADHGLLIRDASGLERLSAVDSLVFDKSGTLTEG
                     KPSVEKVVPADGYESEQVLSLAASLELGSEHPVAAAIVRAARLQKLTLAAVTDFVAQP
                     SGGVSGRVLGRFGVVGSPEFLREQKVDVSSVARAAEGYARAGSTVVFVGALESGFRKC
                     IGFLVLSDPFKKGIHETLRALREEGLELVLLTGDAPETAKSLAAELGIDRVEACLNAV
                     DKASFIEQQRRAGRVVAVVGGGAKDAPALGAADVGFALGTASELAVESAHLTLLKPDL
                     RGLLPALKLARAVRRNVQQNLALAVTYNLVCIPFAAFALYPTFGAVLSPLAASFAMSM
                     SSLSVTWNALRLRSIQL"
     gene            complement(12970..16476)
                     /locus_tag="VFQ61_17050"
     CDS             complement(12970..16476)
                     /locus_tag="VFQ61_17050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9956220.1"
                     /translation="MHDTTPGPPSGKAAGGSGEHPRLQFQAPNLWFTFDASGCCIAAF
                     GKVGCEPQVGAESALGRRFADWVGETECAAAAELERRLLSGSSETASMLVQVQQTRRP
                     PITMHVRAQRVRLQQGYGFSVLARALDPYPEWAPEFAVHRASKAGSGGELAIRGSWYL
                     DLETHQVTWSPEMVELFGIAQPSSIHGVVDCVHVEDRAAMARALEACAQTGTPYRSEY
                     RVVLPDGSLRFVRSRGRRVVGEDGAAIAVVGTSQDMTPQRRVERRYRMLATLAPIGIF
                     EANTEGTCVFVNERWCQITGLRSADALGASLMQVIHPEDRACFEADYKQSLREHRELR
                     AQYRVLRSDGAVAWVDAVISISEDESGGVTCLGTFSDVTQNKEVQGSLHEALALTRGI
                     LESTSYGIVVTDRRGVIRVFNAGAEHMFGYTADEVVGKATPELFHLPSELEQRAKCLG
                     ERLGTTFAKPFDAMTASGAATLPEETEWTYVRKDGARRPMSVSLTALKDPAGRVSGHL
                     GVCADISARKRAELELIRAKETAEAATYAKSEFLARMSHEIRTPMNGVLGMTDLLMQT
                     RLTSEQRGYLQMVKSSAKGLLHVINDLLDFSRIEARKLELENIPFSLRDCLGEAVRNL
                     ALTAQRKGLELVLSLSRTVPNHLIGDRYRLQQIVTNLVSNALKFTSQGQIVVSARVQA
                     LNEREVTLWFSVSDTGIGIPEDKQAVVFEAFAQAEDATTRKFGGTGLGLAICSELVSM
                     MSGRIWVTSAPGRGSEFQFTATFVRIEGAHADACDSYHGMSALVVDDNVASGEALADM
                     LRELGFEVANAADDVQALSAVEALSVCDRQFAVVFVDEAMPSVDTHGLIRELQRRARE
                     AKLIMMGVLLREPEHQRLLRLTKPLELAAVCELLRTALRGSKQPDSAIGPSNDAFSDP
                     PLRVLVAEDNAVNQTLAKAILEKAGHSVHVVENGLMALESLACERFDVVLMDVQMPEM
                     DGLEATRRLREWERVSTLHTPVIALTAQAMKGDADRCIAAGMDAYIAKPIDSDDLMRA
                     VRASWAARRAGVWDPGSRSEQPPCRERDLFSRHKLLARLGGDEDLVREIIELSLLDVP
                     RLMANLQQFVWQAESKQVEQTAHQLKGILLNLSAERASQVAAEVEECARANELSATAA
                     KWSQLAVEIQELERHLRQERIT"
     gene            complement(16579..17691)
                     /locus_tag="VFQ61_17055"
     CDS             complement(16579..17691)
                     /locus_tag="VFQ61_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956221.1"
                     /translation="MARVRSSRRKHLATWLTGALLYAACAQISGLDDYRKVGTGGSTA
                     NGGDTGGQGASGEQGIPNLGGFSSGGDDTGLEAGRAGEVSGSGGAPTSSGGLSGHSGA
                     SVVEAGMAGEISSGGISQVGGAGGQVNLGGEAGSAGEGGTSGRGGSSTIDGAGRGGSS
                     GGATPQTGGHSGQTGGGTGGMSTTGGHAGTGGSSGGAGGTLPNGGTGGGTSCSPRELL
                     VDGGFEKGPKSGAWIDQNIYGQPTILQDPLCRTGSFLAWFGETQSDDSTLHQDIMLPR
                     GTQRVTVSCWYQTAKGIGEAQLQVSFRIGTMESVVAELKNARSSDYAQIQKEFLNPSP
                     GQPVAAALWFHSTSSAEGAAPFRIDDCSVMANVCGP"
     gene            complement(17794..19188)
                     /gene="bioA"
                     /locus_tag="VFQ61_17060"
     CDS             complement(17794..19188)
                     /gene="bioA"
                     /locus_tag="VFQ61_17060"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HET9956222.1"
                     /translation="MAKLQPSATASAPVPASTLVLPRAREMRAGLDRAGISRDALVEL
                     DKRYVWHPYTPMQEYIESAQPLVIRAARGSRLFDMDGRSYIDGNSSWWSAALGHDHPR
                     LVGAMREQLESLTHVALAGITHAPAALLAERLVRIAPGLSRVFFSDDGSTSVEVALKL
                     CLQYWHQNGRPQRTRFVALEQAFHGDTIGAAGVSGVEVFRRPFASVLLDCVRVEPDVE
                     GYDRAFEQLERLLRDESDTIAGVLLEPIVQGAAGMRMYSAEWLRFARQLTARHDTFLI
                     LDEVFTGFGRTGPFWAAQHAGVVPDVLCTAKGLSGGFMPFAATLTSERIFEGFLGSAE
                     RAFYYGHTFCGNPLGASLALEVLDVFEEEGVIEQAQAKANRIAAAFQRFSTHPGVIST
                     RTLGMVAALDLRGGSGYLQTAGKRVCQAAIERGAYLRPLGNTVYITPPLNIPEPDLEQ
                     LLEIFEESLRVACA"
     gene            complement(19269..20873)
                     /locus_tag="VFQ61_17065"
     CDS             complement(19269..20873)
                     /locus_tag="VFQ61_17065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9956223.1"
                     /translation="MIENVWWASVSWGLGAIQLSLPWLLNADSNSIGGTRSRVPNAAS
                     VRAGELARPDTHARLGSGSGVGYGLAAPYAMASLGCGIALLPGAASAWGSVLTVSGLL
                     AGVSWAWREQRHAGREREPRRSPGARWFHGLALAAALGQGLVLGLRGSSHVASAGALS
                     LLAGMIALGAAWAGLSRKPGPRQLGMVSAEAEGAPETGLWRVRSSFHHHVFALGTLAL
                     GLAVIVIGGVDLARGIGFVASGPAISPMLLPHILAMHGVSGVLQVTLDYRERLGLSQL
                     QAGDLMQQAERLQSVEAELARLQRELSSKQQLAAVGELAAAIAHEVRNPLAVIVNAVS
                     GLRRTDLPSRERGMLLDIVEEEAGRLNRLVTDLLRFARPVNVRRSRVSLADLANRCRA
                     TLKDRFDVEVHVADDPSVWTVDVDPNLFRLVFDNLVANACQAMSSGGTVQVQVDPAEL
                     NGSPAARIEIRDQGHGMEPQVLERALDPFFTTRPSGTGLGLPIVQRIVDAHGGQLELD
                     SAEGKGTRVILIVPVRAPEAIASEAS"
     gene            complement(20870..21613)
                     /locus_tag="VFQ61_17070"
     CDS             complement(20870..21613)
                     /locus_tag="VFQ61_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956224.1"
                     /translation="MSASSAPPLSSALASSAAPNARLTRIQLSPTSATLAPPDLRLEI
                     VEPAFAERLDAALAPKSRVRIQVENWPLSADTAGVLVSLDGGRPRRVEASRALELGSL
                     TPDGTVLPGAHVLFAVAVDPQGRALRASPNQPRQPFSVVDFYVGRDPAALPEPRAPRV
                     FCVSPQGTSYATASTEQLLDVYSIGTLSPRLPLHISYRGEHLATTIVALQPYVVRGLP
                     WGDIEFSIDAGASAPAVCRVTVNPEVTTQ"
     gene            21931..>22120
                     /locus_tag="VFQ61_17075"
     CDS             21931..>22120
                     /locus_tag="VFQ61_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956225.1"
                     /translation="MSYLVLARKWRPMRFEDLVGQDHVTRTLGNAIKSGRVAHAFLFT
                     GVRGVGKTTSARILAKALN"
BASE COUNT         4094 a         7214 c         6978 g         3834 t
ORIGIN      
        1 ctcggcggcg cgttcacggt cgatgcgtag cgcaagctcg ggcttacccc cgacgtaagt
       61 gctcgccacg tccaccgctc cgggcacttt gcgcacgcgc tcgacgagct gatccgtgta
      121 cttcgccagc gcgtccagat ccgggccgct gatttggtac tgaatcagcg cggtgcggaa
      181 gccgccgcca ctgaacatgg cgacctgcga gacatccagc gtgaggtctt tgggttggtg
      241 cggcaggatc tgcttgcgta cccgatccat cagctcgtct tgcgagagct tacgtttctc
      301 ggggtcggtc aggcgcacgt acacgcgcgc taggttcggc cgccggtcgt tgttgtcgcc
      361 gacggtcacc agcgtggacg tcacttcggg catcgtgcgc agctcgcgcg ccagtcgctc
      421 acccaagagc gcggtggcgt cgatgctcgt gccctccttg gcgcgcaggt tgacttcgaa
      481 gtgcgcctcg tcactcttcg gcaggaagcc cttcggaacg cgggcgagca acggaccaca
      541 ggcgatcagg gaggccagcg aggccagcac gaccgtgtag cgtcggcgca tcacccaagc
      601 caggaccgcc atataggcgc gttccatcgg caagtagaag cgatcgacga tgcgctcgag
      661 caacactttg cgcggctcgc cggtcgcgcc caccgggcga tgtgcgcgca gccagcgcga
      721 ggaaagcatc ggcgtcagcg tgaagctcac gatcaaagag accaagatcg agaacgccat
      781 cgtcagcccg aagctgcgga ggaaccgccc tacgataccg gacatgaacg ccaccggcag
      841 aaacaccgcc gcgagcgaca aagtggtcgc gagcacggcc aggccgatct cgcgcgtcgc
      901 caggatcgag gccttggtgg gcgagtagcc cttttcttcg atgtaccggt ggatgttctc
      961 cagcaccacg atcgcgtcat cgatcacgat gcccaccgcc agcgcgagcg cgagcagcgt
     1021 gatggtgtcg agtgagaagc cctggatcca catcagcgcg aaggtgccga tgatcgacac
     1081 cgggatcgcg aaggccgcga tcaacgtgct gcgcacgcta cccaggaata acaacactac
     1141 tgcgcccgcg aacagaccac cgagcaacaa gtgctctttg actgctgcaa ccgaggtgcg
     1201 gatcgtactc gtattgtccc gcacgacttc caggctgtaa cccttcggca attcgcgact
     1261 caagtcggac atgcgctcac gcaccgcgtc cacgacggcc acgctgttgg agccggactg
     1321 cttgcgcacg gccaatacca ccgccggttt tccgtcgcgc accgcgaccg tctccggcgt
     1381 ctcgatgcca tcctccacct cggccacgtc cccgagtcgc accacactgc cgtcacgctc
     1441 caagatactg aggaggcgga cttcggaggc atcctccacg cgaccgcgca cgcgcagcac
     1501 ctgctgcgaa ggacccgctt cgatgcggcc cccgggcaac gtcaagttcg cggcgcggat
     1561 agcgcgctcc acgtccacgg ccgtgatccc ataagcacgc agccgcgacg gatccagcga
     1621 cacgttcact tgccgcttgg tgccgcccac gatcgtgact tggcctaccg accgcgcgtt
     1681 ttcgatgcga cgccgcacga tcttgtccgc cacctccgtg atctcgcgcg tgggagcgtt
     1741 cgcgttgacg gcgatgtaaa gcaccggagt tgcgtccggg tccatcttca acacgctggg
     1801 ggcctcgatg ccttcaggca ggttctggag cacgccgttg acgcgttccc gcacttcctg
     1861 cgccgcgacg tcgatgtctt tctcgagtac gaaggtcacg tagacctgcg agacgccctc
     1921 gctcgagacc gacgtcagct cgtcgatgcc gctgatcgtg tttacggcct cctcgatctt
     1981 gtcggtgacg tccgtctcga cctccgaaga ggccgagccc ggtagccgcg ttacgacggc
     2041 cacggtcggg aaatcgacct ttgggaacct gtcgacaccg agctgcaagt acccgacgac
     2101 cccgagcacc aggatgacca ggatcaacac ggttgcaaaa accggtcgcc tcaccgagac
     2161 gttcgccaac cactgcatga gttcttacct gtccttgatc gccgacttca cgtggccgcg
     2221 ggtgcagccc cgatcgcttg cgggatcgcg cagaccccaa tcgagaaatt tggaaggggt
     2281 cggctcggga ccccggcgtg ctcagagcgt gagcgctccg tccaccacct cggcggaagg
     2341 agaggtgacg acccgctcac cgacgtcgag gccagaaatc acgggcacat cgttgccgcg
     2401 gggttccgcg gcgagcacca cgcgctcttc caccctgccg tccttcacta cgaacacgcg
     2461 cgcggccgct cccgcacctc gcacgctctc tttgggtacc accgacagct cttgttcgcc
     2521 ggtgttcagc tcggcctccg cgaacatgcc agggcgcagc gcgccatccg tattgtccac
     2581 caccgcctcg atgactaagt catgagtcga gcggcgcagg accgggccca cgtaacggac
     2641 gctcgcgcga aagcgctttt ccggcaacgc ccgaaccgaa aactgcacgt cccgcccctg
     2701 ctgaaccgca gccgtcgccg actccggaat cgagagttcg agacgcaacg gattgatttc
     2761 caccagggtc gcgatgcggc gcccgggcgt gacgtactcg ccgacgtcca cggcacgatc
     2821 ggttaccact cccgcgaagg gagcccgaat gaaggcgtcc gcgatggctt tgtccgaaac
     2881 gcgcgtccgc gcgagcgcgg cttgcgtcga ggtatccgaa gctcggcagt tggcgcgcgt
     2941 gcggtcgagt tcggcgcgac tcaacgcgct cgaggcaaac agctgctcgg cgcgctcaca
     3001 ctcgagggat gcgttgtctc gatgcacacg cgccgtttcg agctccgcgc tcgcaacggc
     3061 acggctcagc acggccgagc gtgaatccaa acgcgccaga agcgcgccct tctccacacg
     3121 ctgaccgcgc tcgatcgccg actctacgac tcgccccgcg atgtcggccg cgacttctga
     3181 ctcgcgattg gcgatcagcg tgcctgtaat cgtgagagtc ttgggtaaag acttgcgact
     3241 cacctcgacc gtcttgaccg cgatcttttg cgcggcaacg ggcttggcca cagccttctc
     3301 agcagcttcg gcgcgagtgc acgctacggc ggtaaacgac agcaacgtgc cgaaaacggc
     3361 aaacacactc agatggttcg cacgcaggtg gttcgctcga gccatgaacg tcatgccggg
     3421 ccgcctagca cgtagcgggc caagcattta ctaaggggcg tgctagctat tgccgcgaat
     3481 tgcgtggttt ttttatcgga cgtacgtccg tgccggacag ttgtccgggt gcatcaaaga
     3541 cagaacctcg cgccgaccgt tcgaaatgtg tgtagttgag cggggcgctt cgtgaagttg
     3601 ttcgtgggaa cttgactgaa cccgctggtg aagaaactct cgcgccccac acagcgtaca
     3661 agcgcaccag taaaggcttg gccatgcaga aggtcctcgt tgtggacgac caagaagccg
     3721 tccgtaccgc acttcaagtg ctgctcgatg ttcacgacat cccctgcgtg acggcgtcca
     3781 ctcccgaggc ggctctctcg ttggtccgca cggaagacct gggtgcggtg atccaagaca
     3841 tgaacttcgg cgcgggtacg acctctggcc aagagggctt gcgcctgttt cgtgagatcc
     3901 acgcgttgga tcccgaattg ccggtgatcc tcatcacggc ttggacatcg ctcgaaacgg
     3961 cggtggagct gatcaaagag ggcgcctccg attacgtcgc caagccgtgg gacgacgaga
     4021 agttggtggc gagcatccag aatttgctgc gtttgcgggc actcaagctc gaaaacacgc
     4081 gactttgggc gcaagaagag cggctgcgcg gcgaacttgg ccagcgcttc gacctgtgcg
     4141 ggctcgttta tcgaagccga gccatgcatg aagcggtttc gctcgcggta catgtcgcgc
     4201 catccgaggt gccggtgctc atcacgggcc cgaatggttc gggtaaggag aagcttgcgg
     4261 aaattttgca ggccaactca cgcagaaaac gtgagccgtt cgtcaaggtg aatgcgggcg
     4321 ccctgcccga tcagctattg gaagccgagc tgttcggggc ggaggcaggc gcctttaccg
     4381 gtgctatcaa acagcgcgta ggtcgtttcg aagccgcaca cggcggcaca ttgtttctag
     4441 acgagatcgg caacttgtcg gcttctgggc agatgaagtt gttgcgcgtg ctgcaaaccg
     4501 gcgagtatca gcgcctcgga agcagccaca cgcgcaacgt ggacgtgcgc gtgatcagag
     4561 cgacgaacgc cgacttgcgt caagagattg cccacggtcg ctttcgcgaa gatctctatt
     4621 tccggctcaa cgtgattgag attcacgtgc cacctctggc ggagcgctgt gatgacatcg
     4681 ttccactcgc agagcacttc ctggcgctgc acacggtccc cggccgtgaa cgcgccgagc
     4741 tcagcgacga ggccaagttc gcgctggaac agcacgactg gtcgggcaat gtccgcgagc
     4801 tcgagaatcg aattcaacgc gcgttgctgg tgagcggcgg cgagcgcgtc acgccttcgg
     4861 acttgggtct cgaatcgaac ctgaccggcc cgggtcggcc gagctcccct ccccgccccg
     4921 ccaactcgcc cgatagcgaa gagcgtcggg ccgtggaggc cgccctggtc cgtgcccggg
     4981 gggtcgtctc cagggccgcc gcagatctag gtgtaagccg gcaagccctt taccgtcgta
     5041 tggagcggct cggcatcgag ctcgaacgcc gccccaaaac cccctgagtc gtgggggttc
     5101 gaacgcgggc gcgaacggcc cgcagagtga atgcacatga ccgctatcgc caagagcacc
     5161 gtagagaagg ccgacaccat cagccatacg ctggagagtc gcgccgtccc cgagttggct
     5221 caggaccgtc cgagcggcga gcgcactcgt agcaggattc aacgtcgaac catcactctc
     5281 gcactgccga gcggtatgac gccgctcgac agcgaggagg ggttgcgttt tctgagcggg
     5341 cgtttggcca tgttttcggt cgtggtgtgc gcgctgggcg tgttcgcatt actcgccacg
     5401 cagctgctga aactgggctt gccagactac gagtttccac gcacctttct aaccgaaggg
     5461 ggacttcaac acagcgtggc attcgggatc tccctggcga tcttcgcgat agcgcggaac
     5521 tacaagtacg gtttgctggg gctgttgacg ctggacgccg cgggcacgct gttgtcgctc
     5581 tccgcctatg ccgcgatgtg cctgtcgaac atgcgcatgc cgcagcggac cgacatcacg
     5641 atgatgctca ttacgctgag tgtactcacg ctgcgcgcgg tggtgatccc gagttcttgg
     5701 gcgcacacgc tgtgcgtggg agtgctggcg agcctgccgg cgctggtgct cacgtacctc
     5761 aatgctccgc gggtggcggc gctcggactg ccgttcccaa cctggatcgc cctcggctac
     5821 atgggagttt ggaccgtact cggcttgagc atcagcacgt tggcctcgca cgtcatttac
     5881 ggcctacgtt gccagatcgc ccaggtgcgc cagctgggtc agtacgtgct cgaaagcaag
     5941 atcggtgagg gcgggatggg cgtggtctat aaggcccgcc actcgctcct tcgccgaccc
     6001 acggccatca aagtggtgct cccggaacgt gtcggcgagc aggtcttgcg caggttcgag
     6061 cgtgaagtgc agctcacggc gcgcctcaca catcccaata cggtttcgat ctacgactac
     6121 ggtaagtcgc cggacggcaa cttttactac gccatggagt acttggacgg cgtggactta
     6181 gagcagctgg tgtcacgctt cggcccgctc ccgaccgcgc gcgtgattca cgtgttgaag
     6241 caggtggccg cttccttggc cgaggctcac ggcgtcgggc tcgtgcaccg tgacatcaaa
     6301 cccgcgaaca tcatcttgtg tgagcgcggg ggaaacgccg acgttgccaa ggtcgtcgac
     6361 ttcggcctgg tcaaggacgt gcagggactc gggcggaaca agtccgcgag cttgagcacg
     6421 gtacatgcgg tcgtcggaac gccgctctat atggcccccg agtccctggt caatccggag
     6481 cgtgtggacg cgcgcgccga tttgtatgcg ctgggggcgg ttggctacta cttgttgacc
     6541 gggttgcccc tgtttcaggg cgcgtctctg gtggaggttg ccgccaagca cttgcacgag
     6601 cgtcccgtcg ctccctcgga gcgcacgact cttcccattg cgcgagacct ggagagcatc
     6661 gtgctgcgtt gcctcgagaa agattcggca aagcgccccg catcggctca ggagctgttg
     6721 agtcaactgg aagcgtgtga gtccgacacc tggacggccg acctagcaca tgaatggtgg
     6781 aacgagaacg gggaacgagc acgcgccgga cgccagcaag agctcgcgga cgatacaaac
     6841 catcagcacg cgactctttg cgtagacttc gcggcacgct gctgagcggg actgcggtgt
     6901 ggcaacgcct tctccgctga gcgggcccca cggctaggcg acgtggtcag gtcgcccacg
     6961 ccggatgggt gtaggtgatt gttcgagaga ctgctgattt caagacgagg ttgattccac
     7021 gactggggtc gtgtttcgtt gtcggcatgt ttcgcaaagc catgccttcg aaagccttgt
     7081 cttgggtcag cagtctgagc atcgcgttct tcgcagcgca ctgcaccgtc acctcggacg
     7141 atgacgacac cacggcgtgc gagccgaacg agacccgcac ttgctcctgc tcggacggaa
     7201 gcaaaggcac gcaaacgtgc acccccggtg gcacacgctt cggcgcctgc gcaccttgca
     7261 ccggcagcac cggaggggcg ggcggagaga acagcgctgg aggagagagc ggcagcacct
     7321 cgggcggaac aacgagcggt ggcacgactc ataccggtgg cagcggcggc agcaccaccc
     7381 aggccggatc cggtggtgac acgggcggag tcaccagcag cggcggagcc ggtggcaacg
     7441 gcggtgcggg tggcgatgcc aacgtgggtg gggccggagc gctctgtgcc gacgcggtcc
     7501 cggtggataa atgcgacgag tgcctgctct cgcagtgttg cgaagagtac gtggcttgct
     7561 cgggagaagc agagtgttcg gacgtttgga gcgacttcgc aaagtgcgtc gaagatttgc
     7621 gagtcaacgg cgaggctacg cccgccaacg tcaagcagtg tgcggacatc atcaacgtgg
     7681 acgaggacga aaacccgctg cccccctcac ccgccaccgg agctctgatc gactgcatgg
     7741 ggggctcgga tagctggtcg agcgcgagca gttggccgag cacggcttgc acgaccggtt
     7801 gcttcaaacc gtgacgtcaa atgtcctcga ttgcgggtcg cgcgatagaa actgcagacg
     7861 atcagcacgc gcgaacgcgg tacgactggg agatcggaaa tgaacaagta tcgactagcg
     7921 agcattgcgc ttcttgcgct ctgcatcggc agcggcacgg ccgcggcgga tgacgctcag
     7981 accgaacggc agaagcgctt cgaagaactg cggaagaagc gcgaagagca gcgcaaagct
     8041 gcgagcgcgg gcaagggtgg aagtaacgcc ggcggggcaa cctcgaccgg ctccgccgcg
     8101 aagagcggcg gcgcgacggg caagggaggg agctcacatt cgggtggttc cggcgggagc
     8161 gcctccggcg gaggagccgg aaaaggtgga gcgaccgcag ctccaagccc ggattctcag
     8221 ctcgacgagc tgcgcaagac gcggaccgca cgccgacaat cggtgcagcg tgaactccgt
     8281 gagcgctggg gcagcgcgct cgacaacccg cgcagccggg acgagctcaa gacgcacggc
     8341 cgccgtgtcg ccgagctcgc gcgcttacgc tcgctatgcg aacaacaaaa gcgggtggct
     8401 gatctcgcga cggtggacaa gttgctgacg cgcgaggagc tgcgccatgc gagcgcgatg
     8461 aacgatatcc gcgaaggcaa gcccgcggcc gcaccaggag gcaccccatg agcacccgtt
     8521 cccgttttgg ttggcaactc tcgtgtgcaa cgctcgtcgc gatcgcgttt gcctcgtctg
     8581 gctgccgcgg cttgctcaag aagcgcgccc ccgtagaggg cgcgtcggct acacccagca
     8641 ctacgaccgc tcccctagcg agcgcaccgt tgcccaccgc accatcgccc accgcgccat
     8701 tgcccagcgt gccgcccgcg gccaccgccg cggccgtgac tcaagacgcg gacctcccga
     8761 caccgcagga tttcgaggag gacgctttcg aaaagattac cgccaagaat ctgcaggcag
     8821 agctcactcg cttgtccaag gagatcggca actgagctcg agtgcgcgcc gagccgccga
     8881 ggagtcgggc tcgatcccat atgcctcgca gctcggcccc gcgcatttgc gaagggtgcc
     8941 gaggctcggc gccactcaaa gcaggaaaga gaccccgagc gaaaacgttg ggtaacctag
     9001 gcgcatcgtg agggtgacct ggtccgagaa atggaatcgg ccgcccgcat acagcgccgg
     9061 gcttatgctc atgtcgcggt ggcgcggaaa attgagcgac aaccccggct caccaaatac
     9121 cgaccaacgc cgggcgagcc aaaaattcca ctgcatcacg atcggcacgt aaaagtagtc
     9181 gcgactatat tgcgtatcca cctctgcgca gatcagcgtg ccgttcggac cgtcgatgaa
     9241 ccgactgcag cggccccgcg ccgcgctcgt cgagtagtgc acccaatcca tcccgaagcc
     9301 gacgcccacc gaatcgttga gcttggaaat gaagccctcc ggtgcaacct cgatggtgcc
     9361 gcggaagcca agccccaagc cgtccccgcc gtgagcgggg cccggtggat cgaacgcccc
     9421 gagcaagaga tggggttcca gttcgaagtc gtaatgcggc cgctgaccag ggtgacgaat
     9481 cgtgctctgc gcgtgagcgg tgccgatttg gccgaagcac cacagtgtgc acgctgcgaa
     9541 gatgaactga gagcgtttca cgagttctcc ttaatttcgc cggtctctaa tgcgcggact
     9601 gtctttttgc gactgaagtt cgctggcgct ggggatcagt tggagcaggg cccgcaagcg
     9661 agcactccgt tgccgcaatc cacggccccg ctaccgtcgg cgcatgtcac ttcgcaacct
     9721 cggccgccga cgcgttggca cgtgaccaaa caaaaaccag cgcactccac gacgcaggac
     9781 gacacatcgg cgcacgtcac acggctgttg cgcccaacgc gcacgccgca cgcattggcg
     9841 cgggtgcaac ggtagctgca gccatcacca caggccgcgc cgcaacgatc cgtatcgtga
     9901 cagtcgatgt tgcagttttg ggagcagtag gccgagcatt cgtgcgtgcc gtagcaagcc
     9961 gcgtcgcagt tgtcttcaca gaccgagccg cagcgatcca tgtcgtgaca actctggcgg
    10021 caagcatccc cagaacaagc caggtaacac tcctgccctc cggcgcagtc gcagaaatca
    10081 ccttcgtcgc agttcccacc cgagtcacaa gccgtggcca gcgccgcaga aaccaaggcc
    10141 aaagcgaacc accaaagcgg gcccttggta atcaacgcac gtttggtacg cgctggcgca
    10201 gatctcgagg ccctcgagag catggtcttc gaggtggtgg agctgcgctg gtgggccagg
    10261 ggggaatcta gagcgcgatc cgcggcagga tcgagagtgg gatgattcgt ggggcacggc
    10321 gtcatcgcgg cgaagcatag tttcgagctt ctacaaaccg aaggggaatt tggaggagct
    10381 cctcctacat tggccgttca gcctggatcg tcgactaccc gaggaaccag aagttgggtt
    10441 ttcttaccta agcgcgctaa ccgcgttcgg gctttctcac ccgaggcagt gtccccccgc
    10501 cccgatatgc cggggagcca tctggaacgc gcttcgatct tccactgctg ggccacccgc
    10561 gggagggcag agccgaattg aaaagttcca ccgacgatca ctcccaaggg tgaaaccaga
    10621 agctcccaag ggtgaagcca gaagcagagc cagattacat gctgcgccga tgtacccgcc
    10681 agaccgactc gaactccgct cgatcgagct ccccataacg cgaaatggcc aaggcggaaa
    10741 tgcgcagcca gaacttgctt gtcccggctg gaagggataa cgctcggaac ggcgattctc
    10801 ggccgcacgc tgccggtagc tgtcggggct cattgcagtg actgacaccc attcggaccc
    10861 caaagtacga agctacgcgg tcgcggctcc gcccgtctcc aggggcgcac ggcccactcg
    10921 ctcgagctcc cctcctctcg gtaccacgga tcgcgagtcc agcgcgctca agcgccggtt
    10981 ctggacggcg gtagccgggt tcgtgccgat cgcgttcttg gagctcgggg cgggcgcgat
    11041 ttcgagttcg ttgcgcgcct ggctcgagtg cttgcttgcg tgtcctgcgg tcttctactc
    11101 cgcggcaccg ttcttcgtcg acgccggaag atccgttcgt cgagcgcgcc ccgacatgtt
    11161 cgcgctgatc gcactcgggg tggcggcggc ctttggtttc agtgtgctgg ggctgctgat
    11221 gccaacgcta ctgcccgccg ccgcacgggg cacgtactac ttcgaagcgg ccacgggcat
    11281 cgtggcgctg gtgttgttcg ggcaactcct ggagctgagt gcgcgacgcg gcacgcgccg
    11341 ccatctggaa gcgttgcaaa acctgctgcc caacagcgct cggcgcgtgg ctcccgatgg
    11401 cagcgagcag cgcgttccgt gcacacagct gctgccccac gatctcgtac ggatttgccc
    11461 aggcgaacgg cttgcggcgg acggcttgat cgtggaaggg gaaggacagc tcgacgagag
    11521 cttgctcacc ctcgagaagg cgccggtcaa gaagggcccg aagcagccgg tgcacgcggg
    11581 cacgctcaac actggcggca cgttcacgat gcgtgcggag cgcttgggtt cgaacacgtt
    11641 gctctcacgt atcactttgg cggtaagcca agcccagtcc gctcgcggct ctttgcagcg
    11701 agaggcggat cgcatggcgg catggttgat cccgagcgtg atcgctctgg cgctgattac
    11761 tctcgggtgg tggctcgggt gggcgccggg cgtcgcaccg gacgcgcgct tggcccacgc
    11821 tttggtgagt gcgatcgcgg tgctcgtggt ggcgtgcccc ggcgcgttgg cattggcggc
    11881 gccgctgtcg atgagcatcg cggcggggcg aggcgccgac cacgggctcc tgatccgtga
    11941 cgcgagcggg ctcgagcggc tttccgctgt cgacagcttg gtcttcgaca agagcggcac
    12001 cttgacggag ggcaagccga gcgtcgaaaa ggtggtcccc gccgacggct acgagagcga
    12061 gcaggtactt tcgttggcgg cgagcttgga gctcggcagc gaacaccccg ttgcggccgc
    12121 gatcgtgcgc gccgcgaggc tccaaaaatt gaccttggcg gccgtgacgg acttcgtggc
    12181 acagccgtcg ggaggtgtct ccgggcgtgt gctcgggcgc ttcggcgtgg tgggctcgcc
    12241 ggagttcctt cgagaacaaa aggtcgacgt ttcctccgtc gccagggcag ccgagggcta
    12301 tgcgcgcgcc gggagcacgg tggtcttcgt cggcgcgctg gagtccggat ttcgcaaatg
    12361 catcggtttc ttggtgctga gtgatccttt caagaagggc atccacgaga cgctgcgggc
    12421 gctccgagag gaaggactgg agctcgtact gttgacgggg gatgccccgg agacggcgaa
    12481 gagcctggcc gccgagctcg gcatcgaccg ggtcgaggcg tgcttgaacg cggtggacaa
    12541 agcgtcgttc atcgaacagc aacgccgcgc cggacgagtg gtggccgtag tcggaggcgg
    12601 cgccaaggac gcgcccgctt tgggcgccgc ggacgtggga ttcgcgctcg gcaccgcgtc
    12661 ggagctcgcc gtggagagcg cccacctgac cttattgaag ccagatttgc gcggtctgct
    12721 gccggccttg aagctcgctc gggccgtgcg ccgcaacgtg cagcagaatc tcgcgctcgc
    12781 ggtcacctac aacttggtct gcattccgtt tgccgcattc gcgctctatc ccacgttcgg
    12841 cgcggtactc tcgccactcg cggcgagctt cgcgatgagc atgagctcct tgagtgtgac
    12901 gtggaacgcg ctgcgtctgc gcagcattca gctatagccc cggcgggcgt gagcccgggt
    12961 agctcgctct caggtgatgc gctcttgccg caagtggcgc tcgagttcct ggatctctac
    13021 cgccaactgc gaccacttcg cggcggtcgc actcagctcg ttggcacgtg cacattcttc
    13081 cacctcggcg gcgacttggg acgcacgctc cgcggaaaga ttgagtagga tgcccttcaa
    13141 ctggtgcgcg gtttgctcta cttgcttgct ctcggcttgc catacgaatt gctgcaagtt
    13201 ggccatcaaa cgtggcacgt ccagcaacga aagctcgatg atctcgcgca ccaagtcttc
    13261 gtctccgccc agtcgagcaa gcaacttgtg acgactgaat agatcgcgct cgcggcaagg
    13321 cggttgctcg ctgcgcgacc cggggtccca gacgccagcg cgccgcgcgg cccaggaggc
    13381 ccgcacggct cgcatcaggt cgtcggagtc aatcggcttg gcgatgtagg cgtccatacc
    13441 cgcggcgatg caacgatccg cgtcgccctt catggcctgc gccgtgagcg cgatgaccgg
    13501 cgtgtgcagg gtgctgacgc gctcccattc gcgcaagcgt cgagttgctt ccaggccatc
    13561 catctctggc atttggacgt ccatcagaac cacgtcgaag cgctcgcagg cgagcgactc
    13621 gagcgccatt agaccatttt cgacgacatg caccgagtgg cccgccttct cgaggatggc
    13681 cttggccaag gtctggttca cggcgttgtc ttctgcgacc aatacccgca gaggcggatc
    13741 cgagaatgcg tcgttcgaag gaccgatcgc cgagtctggt tgcttcgagc cacgcaaggc
    13801 ggtgcgaagc aactcgcaca ccgccgcgag ctccagcggt ttggtcagcc gcagcaagcg
    13861 ctggtgctcc ggttccctga gcagcacccc catcatgatt agcttggctt cgcgggcgcg
    13921 gcgctgcagc tcccgaatta gaccatgggt atcgacgctg ggcatcgcct cgtcgacgaa
    13981 caccacggca aactgccgat cgcagaccga tagtgcctcc accgccgaaa gggcctgcac
    14041 gtcgtccgca gcgttcgcga cttcgaaccc gagttcccgg agcatgtcgg ccagggcttc
    14101 gccgctcgcc acgttgtcgt cgaccacgag ggcagacatg ccgtgatagg aatcgcatgc
    14161 atcagcgtgg gcgccctcta ttcgcacgaa ggtcgcggtg aattgaaact cggagcctcg
    14221 gccgggcgcg ctggtcaccc agatgcggcc actcatcatc gacaccaact cggaacaaat
    14281 cgcgagaccg agtcccgtgc ccccgaactt gcgcgtggta gcgtcctcgg cttgcgcgaa
    14341 cgcctcgaag acgaccgcct gtttgtcttc gggaatgccg atcccggtat cggaaacgct
    14401 gaaccagagc gtcacttccc gttcattgag cgcctgcacc cgagccgaca ccacgatttg
    14461 gccttggctc gtgaacttca acgcgttgct gaccagattc gtgacgatct gctgcagccg
    14521 atagcggtct ccaatcaagt gattcggcac cgtgcgcgag agactcaaca ctagctcgag
    14581 gccttttcgt tgagcggtca gcgctaggtt gcgcacggcc tcccccaagc agtcacgcag
    14641 cgagaacggg atattctcga gctcgagctt tcgggcctcg atgcgtgaga aatccaacaa
    14701 atcgttgatg acgtgaagca agcctttggc ggaagacttg accatctgca ggtagccccg
    14761 ctgctcgctc gtgagccgag tctgcatcaa caaatcggtc ataccgagca cgccgttcat
    14821 gggcgtgcga atctcatggc tcatacgcgc caggaactcg gatttggcgt aggtagcggc
    14881 ttccgcggtc tctttggcgc ggatcaactc gagctccgcg cgtttacgcg cgctgatgtc
    14941 ggcacacacg ccgaggtgcc cggagactcg acccgcgggg tccttcagcg cggtgagcga
    15001 aactgacatg ggccttcgcg caccgtcttt gcgaacgtag gtccactcgg tctcttcggg
    15061 cagggtggct gccccgctcg ccgtcatggc gtcgaacggt ttggcgaacg ttgtgccaag
    15121 ccgttccccg aggcacttgg cgcgctgttc gagctcggat gggagatgga atagctcggg
    15181 cgtggccttc cccactactt catcggcggt gtagccgaac atgtgctcgg cgccggcgtt
    15241 gaagacccgg atcacgcctc gcctatcggt cacgacgatg ccgtagctcg tgctctcgag
    15301 gatgccgcgg gtgagcgcca aggcttcgtg cagcgagccc tgcacctctt tgttttgagt
    15361 tacgtcggag aacgtcccca aacacgtcac gccccccgac tcatcctccg aaatcgagat
    15421 gaccgcgtcg acccacgcca cggcgccgtc ggatcgcaat actcgatatt gggcccgcag
    15481 ctcgcggtgc tcacgaagag attgtttgta gtccgcctcg aaacaggcgc gatcttcggg
    15541 atggataacc tgcatgaggc tcgcgccgag agcatccgcg gagcgcagcc cggtgatttg
    15601 gcaccagcgc tcattgacga aaacgcaggt accctcggta ttggcttcga agattccgat
    15661 cggagccagg gtcgcaagca tgcgatagcg ccgctcgacc cggcgctggg gcgtcatgtc
    15721 ttgagaggtg ccaacgaccg caattgccgc gccgtcttcc cccaccacgc ggcgcccgcg
    15781 gctccgcacg aagcgcaaag agccgtccgg cagaacgact cggtattcac ttcgataggg
    15841 ggttcccgtc tgagcacagg cctccaaagc tcgggccatg gccgcgcgat cctcgacgtg
    15901 cacgcaatcg acgacgccat gaatcgaaga cggttgcgcg ataccgaaca gctcgaccat
    15961 ctccggtgac caagtcacct gatgggtctc caaatccagg taccacgagc cccggatcgc
    16021 gagctcaccg ccgctgcccg ctttggacgc ccgatgaacc gcgaactcgg gcgcccactc
    16081 tggataagga tcgagagcgc gcgccagcac cgagaacccg tagccctgct gcaagcgcac
    16141 gcgctgggct cgaacgtgca tggtgattgg agggcgtcgc gtctgctgca cctgaacgag
    16201 catcgacgcg gtttcggagc ttccgctcag tagccgccgc tcgagctcgg ccgccgccgc
    16261 gcactccgtt tctcccaccc aatctgcgaa gcgtcgcccc agggcgctct cagcgccaac
    16321 ttgcggctcg caacctacct taccgaatgc tgcaatacag cagccactcg cgtcgaaggt
    16381 gaaccagaga ttcggcgctt gaaactgcag gcgtggatgc tccccggacc caccggcggc
    16441 tttccccgac ggggggccag gcgttgtgtc gtgcaaatcc acagatccga tatacgcgca
    16501 aaccccactc gagtttagaa cctatcccat tcggaacggc ggacgcggtg ggccgagagc
    16561 gaagcggcgg taatgcgctc acggtccgca gacgttcgcc atgaccgagc aatcgtcgat
    16621 ccggaatggc gcagcgcctt cggcgctaga cgtcgagtga aaccagagcg cggcggcaac
    16681 gggctggccc ggagaagggt tgaggaactc cttctggatt tgggcgtagt cgctcgatct
    16741 ggcgttcttc agctcggcca ctaccgattc catcgttcca atcctgaacg agacctgcag
    16801 ctgggcctca ccaatacctt ttgcggtttg ataccaacag ctcaccgtca cgcgttgagt
    16861 cccgcgcggc aacatgatgt cttggtgaag ggtggaatcg tcggactggg tttccccaaa
    16921 ccaggccaga aagctgcccg ttcggcacaa tgggtcctgg agaatcgtgg gttgaccgta
    16981 gatgttttgg tcgatccacg ccccagactt cggacctttt tcgaatccac catcgaccaa
    17041 cagctcacgc gggctgcaac tcgtgccacc gccggtgccc ccgttgggca atgtgcctcc
    17101 cgctccgccg ctcgatcctc ccgtgccggc atgacctccc gtcgtgctca taccgccggt
    17161 gccaccaccg gtttgccccg aatggccgcc ggtctgtggg gttgcaccgc ctgaactgcc
    17221 tccacgccct gcgccgtcga tcgtcgagct accgccgcgg ccagacgtgc ccccttcccc
    17281 ggcggatccc gcttcgccgc ctaggttgac ttgacccccg gcacctccga cttgggatat
    17341 gccgccgctg ctgatttcgc cagccatgcc agcttccacg acggaggcgc cggagtgccc
    17401 gctgagccct ccagaagaag tcggcgcacc gcccgatccg ctcacttcgc ctgctctgcc
    17461 ggcttcgagc cccgtgtcgt ccccgccgct ggagaagccg cccaagttgg ggattccttg
    17521 ctcccccgac gcaccttgcc cacccgtgtc tccgccgttc gcagtggagc caccggtgcc
    17581 gactttacga tagtcgtcga ggccgctgat ctgggcacaa gccgcgtaga gcaaagcgcc
    17641 cgtgagccag gtagcgaggt gtttgcggcg agagcttcgg acccgtgcca tgtgcattgg
    17701 agcgtgcctc aaacgagggt ctgctcgcta tgtgaatctt cttccgtagt cgattgcggc
    17761 tcgggagtgc cacgaatacg gcagttcgaa gcgttacgcg caagctaccc gcagcgactc
    17821 ctcgaagatc tcgagcagct gctcgagatc cggctcggga atattgagcg gcggcgtgat
    17881 gtaaacggta ttgcccagcg gccgcaggta cgcaccacgc tcgatggcag cctgacacac
    17941 gcgcttgccc gcggtttgta agtagccaga gccgccgcgc agatcgaggg ccgccaccat
    18001 ccccagcgtg cgcgtagaaa tcacgccggg atgagtcgag aagcgctgaa aggcggctgc
    18061 gatgcggttc gccttggctt gcgcttgctc gatcacgcct tcttcttcga acacgtcgag
    18121 cacctccagc gcgaggctcg cgcccagtgg gtttccacag aaggtatgcc cgtaatagaa
    18181 ggcccgctcc gcagagccca gaaagccctc gaaaatccgc tcactggtca aggtcgcggc
    18241 gaacggcatg aagccgccgc tcaaaccttt ggcggtacac aacacgtcgg gcacgacccc
    18301 ggcgtgctgc gccgcccaaa acggcccggt tcgcccgaac ccagtgaata cttcatccag
    18361 gatcaggaag gtgtcatgcc gcgccgtcag ctgccgagca aaacgcagcc actcggccga
    18421 gtacatgcgc atgcccgcag cgccctgcac gatgggctcg aggaggacgc ccgcgatcgt
    18481 gtcggactcg tctcggagca ggcgctcgag ttgttcgaac gcgcgatcat aaccttcgac
    18541 gtcgggctcg acccgtacac aatcgagcag aaccgaggca aagggccgac gaaagacctc
    18601 taccccggag acccctgctg cgccgatcgt atcgccatga aatgcctgct ccaacgcgac
    18661 gaagcgagtg cgctgcgggc gaccgttttg atgccaatac tgcaaacaca gcttgagtgc
    18721 gacttcgaca gacgtcgatc cgtcatcgct gaagaacact cgtgataggc cgggagcaat
    18781 gcgcaccaat cgctccgcga gtaacgcggc aggagcgtga gtgattccgg cgagcgctac
    18841 gtgcgtgagg ctctccagct gttcccgcat agcgccgacc agacgcgggt ggtcgtgccc
    18901 caatgcggcg ctccaccagg acgagttacc gtcgatgtag cttcgcccgt ccatgtcgaa
    18961 caagcgcgaa ccccgcgccg cgcgaatgac cagcggttga gccgactcga tatactcctg
    19021 catcggagtg taagggtgcc aaacgtagcg cttatcgagc tcgactaagg catcccggct
    19081 gatccccgct cgatctagac cggcgcgcat ctccctcgcg cgcgggagga ccagggtcga
    19141 agccgggacg ggtgcagatg cggtggcact gggttggagc ttagccatcg agatcggtcc
    19201 agacatggca ccagacagcg tggccgtcta gctccccgcg cgtcgcgcaa caccgccacg
    19261 accggcactc aactggcttc gctagcaatc gcctcgggcg cccgaaccgg cacaatcaag
    19321 atcacgcgtg tgcccttccc ctctgcgcta tcgagttcta gctggccccc gtgagcgtcg
    19381 acgatgcgct gcacgatcgg caagccgagc cccgtgccgc tcggccgtgt cgtgaagaac
    19441 ggatccaagg cccgctcgag cacctgaggt tccatgccgt gcccctgatc acgaatctcg
    19501 atgcgcgcag ccgggctccc gttcaattct gcggggtcga cttggacctg cacggttccg
    19561 ccagagctca ttgcttgaca cgcgttcgcg accaagttgt cgaacaccag tcgaaacaag
    19621 ttgggatcga cgtcgacggt ccaaacgcta gggtcatcgg cgacgtgcac ttccacatcg
    19681 aaacgatcct tcaaagtggc ccgacaacga ttcgccagat cggccaagct gacccgcgag
    19741 cggcgcacat tgacgggacg agcaaaacgc agcaggtccg tgactagccg attcaggcgc
    19801 cccgcctctt cctcgacgat gtcgagcagc atgcctcgtt cgcgactagg caaatcggta
    19861 cggcggagcc ccgagaccgc attcacgatc acggccaaag gattgcgtac ctcgtgcgcg
    19921 atcgccgccg cgagctcgcc caccgcggcc aattgctgct tgctcgaaag ctcccgctgt
    19981 aggcgagcga gctctgcctc gacgctctga agccgctctg cctgctgcat caagtctcct
    20041 gcttgcagct ggctaagccc caggcgctcg cgatagtcga gcgtcacctg cagcacgccg
    20101 ctcacgccgt gcatcgccaa gatgtgcggc agcagcatag gtgagatggc ggggccgctc
    20161 gcgacgaagc cgatgccacg cgccaaatcg acaccaccga tgacgatgac agccaagccc
    20221 aaggccaacg tgcccagggc aaatacgtga tgatggaagg agctccggac gcgccagagc
    20281 ccggtttccg gcgctccctc tgcttctgcc gacaccattc cgagttgccg cgggcccggc
    20341 ttgcgcgaaa gccccgccca cgccgcgccc agcgcaatca tacctgccag caaactgagt
    20401 gcgcccgcgc tcgcgacatg gctggatccg cgcagcccca gtacgagccc ctgccccaga
    20461 gcggcagcca gggcgagccc gtggaaccat cgcgcacccg ggcttcgacg cggctcacgt
    20521 tcgcgcccgg cgtgccgctg ttctcgccac gcccagctca cgcctgctag caacccgctc
    20581 acggtgagca ccgaacccca agcgctcgcc gcgcctggaa gcaaggctat tccgcagccc
    20641 aggctcgcca tggcgtatgg agcggctagg ccgtatccaa cacccgagcc cgatccgagg
    20701 cgggcgtggg tgtcgggccg agcgagctca cctgcgcgga cgcttgccgc gttcgggaca
    20761 cgcgagcgag taccgccgat cgaattggag tcggcgttca acagccacgg caagctcaat
    20821 tgaatggccc ccaatcccca gctgaccgac gcccaccaga cattttctat cactgcgtcg
    20881 tgacctccgg gttcaccgtc acacggcaga ccgctggggc gctcgctcca gcgtcgatcg
    20941 agaactcgat atcgccccag ggcaacccgc gcacgacgta gggctgcaac gccacgatgg
    21001 tcgtggcgag gtgctctccc cggtaagaga tgtggagcgg caagcgcgga ctgagtgtgc
    21061 ctatggagta gacatcgagc aactgttccg tgctggccgt cgcgtaagac gtaccttgcg
    21121 gactgacgca aaacacacgc ggcgcccgag gctccggtaa agctgcagga tcccggccaa
    21181 cgtagaaatc cacgactgag aacggttgac gcggctggtt agggctcgcg cgcaacgctc
    21241 ggccttgggg atctaccgcg accgcgaaca agacgtgagc gccaggcagc acggtcccgt
    21301 ccggcgtcag ggagccgagc tctagggcgc gactggcttc cacccgtcgc ggcctcccgc
    21361 catcgaggga gacgagtaca cccgccgtgt cagccgacaa gggccagttc tcgacctgga
    21421 ttcgaacccg acttttcggc gcgagcgcgg catcgagccg ctcggcaaat gcgggctcga
    21481 cgatttctag acgcaagtcg ggtggggcga gcgtcgcgct cgtgggggat agttgaattc
    21541 gagtcaaacg cgcgttggga gctgcgctag aagcgagcgc agaagagagc ggcggcgcgc
    21601 tactggcgga cacgaccggc actggacctt cttgtcgtgc gtgccgagcg gggctcgctg
    21661 agcatcctgg gccgatcatc gcgagcgcaa gcaacgcccg cgagtgcgca atgcggaggg
    21721 ccaacacgag cgtagtgtaa ccccgtccag cgggcacctt ctggtaaatg atgcgcgccg
    21781 tcacgtcgag ccacgagcaa accgaccgcc cgggtgccct ccgggcttcg agcatgtcgc
    21841 cgcacaggcc agaggcttgc tcggctccgg acgcagctgc gccccgcggc ctttcagcac
    21901 cggggttgat ctgctagctg aaggccaccc atgagttacc tggtcctagc ccgcaagtgg
    21961 cgtccgatgc ggttcgagga cttggtcggc caggaccatg tcacgcgcac gctcggaaac
    22021 gcgatcaaga gcggccgcgt cgctcatgct tttctgttca cgggcgtacg cggagtcggc
    22081 aagacaacca gcgcgcgcat cttggcgaag gcgctgaatt
//
LOCUS       DASTKU010000494         3777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1655275, whole genome shotgun sequence.
ACCESSION   DASTKU010000494 DASTKU010000000
VERSION     DASTKU010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655275"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VFQ61_17080"
     CDS             <1..404
                     /locus_tag="VFQ61_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956226.1"
                     /translation="IFADDQRIEFHEDVSAVPTADIVFMSGVLQYIEDYPGVLRDLTE
                     RFHPRLFVLTLLPTGSFPTFASAQVNLEGSTMPAWFFNEAALLDQFGALGYELVLRGA
                     ATLEFDMSDFDPAHRLDGMSNWILRAKSARK"
     gene            423..1997
                     /locus_tag="VFQ61_17085"
     CDS             423..1997
                     /locus_tag="VFQ61_17085"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HET9956227.1"
                     /translation="MARSKALAVQTVFVTLLSFLGLLLGLGNSLLIAYLYGASPERDA
                     YLLAAIVPTYLVSLFSGNISILFIPVFIQNHTKDEPQAWRFASSIICYSGLILLAIVA
                     LATTFAKDVVARLAPAFTPAELDLTTRLLRVMMPSALFGGLSSVASSLYYAEQRFLRP
                     NITALLAGSLPLITNLTLHHRMGMFSLAWGTTLGSAAGLLLLLPGLLAPGKLRFSLNA
                     REDGILNLLRSSVPLLLASLLYRFNTGFERILGARLPTGSISFLAYATQIVTMLSSLT
                     SSGIATTFFPALSRAFASNDMPGARRYLAIGVRAVLIVALPASVLLIVFAHPIVQLLL
                     ERGAFDRRATSGVSNSILLLLAGYVASSLGNVVTKCIYVAQKTAWLAPYTIIETIGYV
                     ALGAWLSKYFSYLGLAAASSARDCISVSLYLAASSYLMGGIQGREMWLDAAKLVASSL
                     VMAISVLPLRWWLAQQSPNRLTSLVVLCLSGVIAGAVYFVMTVRVFRIEEGVRMFSAI
                     AARLPGLKRLVPSSRV"
     gene            2006..>3777
                     /locus_tag="VFQ61_17090"
     CDS             2006..>3777
                     /locus_tag="VFQ61_17090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9956228.1"
                     /translation="MKVHLISYEDTQAWILGQLTTHLAEALQVLGVQTSIGRRADPSA
                     DINHHVIYLGYDGKPSRNDTLMITHVDTEAKVRLLSEQLRVARMGICVSSDTMNKLIA
                     LGVPQSRLSFVNYPSPQTGQIKRRKLHIGITSRCYNYGSKREYLLGELAPHLPPDLFV
                     FHIMGSGWDVVMQALSRHGSEVVFYGDFDAVAYRELWQKLDYYLYLGHDEGSTGLLDA
                     LEAGVPTIATPQGFHVDVGVGLVHAFNDITELRLIFQKLADERLALRNSAAHINWPAY
                     AREHLAIWERVLAEGNPAREPAQESKRVDSNPKHAWVSAMPQQPGPGGMAHRPRLLVI
                     ADEPVSDALRVWRNLAALAPPDLRVMVTADGQLLTEDVYRAPAGLEVRIVDLRTRATV
                     DLVQLSLDFDAVLLWDSAPFAVVDALLARRKPNYLLRTEALARLSARYLPFQSMPRRS
                     EEPFYGLVRRLTAHSPARVMAAWTGSPGQAFGSEYEPSRIRAHHSRMAAVFFGDEAWA
                     PLIPNSNKVRFSVPSGDRVSGSNGVPSVSNQTPSKDTSVFFEALFEVIGAPQLSPKPD
                     TLLWLSNKARALVTHVERASRAAR"
BASE COUNT          672 a         1194 c         1099 g          812 t
ORIGIN      
        1 ggatcttcgc cgacgatcag cgcatcgagt tccacgaaga cgtgtcggcc gttcccacgg
       61 ctgacatcgt cttcatgagc ggcgtcctac agtatatcga ggactacccg ggggtcctac
      121 gtgacttgac cgaacgcttc catccccggt tgttcgtact caccctgctg ccgacgggat
      181 cgttcccgac gtttgcgtcc gcgcaggtga acctggaagg tagcacgatg cccgcgtggt
      241 tcttcaacga ggccgcactg ctcgatcagt tcggcgctct cggttacgag ctcgtgttgc
      301 ggggggccgc cacgctcgag ttcgacatga gcgacttcga tccagcgcat cgcctggatg
      361 gcatgtccaa ctggattctt agagcaaaaa gcgcgcggaa atagccttag atccgagtcc
      421 gcatggcccg ttcgaaagcg ctcgccgtcc aaaccgtttt cgtcacgctg ctctcttttc
      481 tcgggctcct gctgggtttg ggcaacagtc tgttgatcgc ctacttgtac ggcgcgtcac
      541 cggagcgtga tgcctacttg ctcgcggcga tcgtacccac gtacttagtg tcgctcttct
      601 cgggcaacat ctcgatcctc ttcatccctg tcttcatcca aaatcatacc aaggacgaac
      661 cccaggcctg gcgcttcgca agcagcatca tttgctacag tggtctgatt cttttggcca
      721 tcgtggcgct cgcgaccacc tttgccaaag acgtcgtcgc aagactcgcg cccgcgttca
      781 ccccagccga actggacctc acgacacggt tgttgcgagt gatgatgccc agcgctcttt
      841 tcggtggcct ttcgagcgtc gcgtcgagcc tctactacgc cgaacagcgc tttctgcggc
      901 ccaatatcac cgcgctgctg gcgggctcac tgcccttgat tacgaacctg accctccatc
      961 accggatggg aatgttcagc ctagcttggg ggaccacgtt gggcagcgcc gcaggattgc
     1021 ttctgctcct gccggggctg ttggcacctg gcaaactgcg cttcagtctc aacgcgcgcg
     1081 aggacggcat cctcaacttg ttgcggagct ccgttccgct attgctcgca agccttctat
     1141 accgcttcaa cactggcttc gaacgcatcc tcggcgcgcg gcttccgaca ggaagcatct
     1201 cgttcctggc gtacgcgacg cagatcgtca cgatgctgtc atcgcttacc tcatcgggca
     1261 tcgcgaccac gtttttcccc gcactatcgc gcgccttcgc cagcaatgac atgcccggcg
     1321 cgcgccgcta cctcgcgatc ggcgtgcgcg cggtgctgat cgtcgccttg cccgcgagcg
     1381 ttttactcat tgtattcgct caccctatcg tccagctcct tctcgagcgc ggagccttcg
     1441 accgccgagc gacgtcgggc gtgagtaact ccattttgtt gctgctagcg ggctacgtcg
     1501 cctcctcact cggcaacgtc gtcaccaagt gcatttacgt agcccaaaag accgcgtggt
     1561 tggctccgta tacgattatc gaaaccatcg gctacgttgc cctcggagcc tggctcagca
     1621 agtacttctc gtacctcggt ttggccgccg ccagctcggc gcgcgactgt atcagtgtat
     1681 ccttgtacct ggcggcctcc agctacttga tgggcgggat tcaaggccga gagatgtggt
     1741 tggatgccgc caaactggtc gcttcgtcgt tggtcatggc gatctcggtt ttgcctttac
     1801 gctggtggct ggcgcaacaa tcaccgaatc gtctaacgtc tttggtcgtg ctgtgtctgt
     1861 ccggtgtgat cgccggcgcc gtctacttcg tgatgaccgt acgtgttttc agaatcgaag
     1921 agggagtgcg aatgttctcg gcgatagcgg ctcgactacc gggcctgaag cggctcgtcc
     1981 ccagctcgcg ggtttgataa tggccatgaa ggttcacctc atcagctacg aagacacgca
     2041 agcctggata ttggggcagc tcacgactca cctcgccgaa gctttgcagg tgctcggggt
     2101 tcagaccagc atcggccgcc gtgcggatcc gagcgccgac atcaatcacc acgtgatcta
     2161 tctgggctat gacgggaagc cgagccgcaa tgatacgctg atgattacgc acgtggacac
     2221 cgaagccaag gttcggctgt tgagcgagca gctccgggtc gcgcgcatgg gcatctgcgt
     2281 ctcttcggat acgatgaaca agttgattgc gctcggggtt ccgcagtctc gactgagctt
     2341 cgtgaactat ccgtcgcctc agaccggaca aatcaaacgc cgcaagcttc atatcggaat
     2401 cacgagccgc tgttacaact acggaagcaa acgagagtac ctcctcggag agctcgcgcc
     2461 gcacctccct ccggatctgt tcgtattcca catcatgggc tcgggctggg acgtggtgat
     2521 gcaggcgctc tcacgccacg gcagcgaagt agtgttctac ggcgacttcg atgcagtcgc
     2581 ttatcgggag ctttggcaga agctcgacta ctatctgtac ctcggccacg acgaaggttc
     2641 gacgggactg ctcgatgctt tggaggcggg tgttcccacg atcgcgacac cgcagggctt
     2701 ccacgtggac gttggagtgg gcttggttca cgccttcaac gacatcaccg agctccgact
     2761 gatttttcaa aagctagcag acgaacgcct tgcgctgcgg aacagcgccg cgcacatcaa
     2821 ttggcccgct tacgctcgcg agcatttggc aatatgggaa agggtgttgg cggaggggaa
     2881 ccctgcccgg gagcccgcgc aggagtcgaa gcgcgtcgac tcaaacccga aacacgcttg
     2941 ggtctcggcg atgccgcagc aacccgggcc gggggggatg gcacatcggc caagattgct
     3001 cgtgatcgcg gacgagccgg tgtccgatgc gctcagagta tggcgcaacc tggcggccct
     3061 cgcgcctcca gacctgcggg tgatggtcac cgcggacggt cagctgctga ctgaggacgt
     3121 gtatcgcgcg cctgcggggc tagaggtgcg gatcgtagac ctacgcactc gagctaccgt
     3181 cgacctggtg cagctctcgc tggatttcga cgcagttcta ctgtgggact cagcgccgtt
     3241 cgcggtcgtg gatgccctac tggctcgtcg caagcccaat tacttgcttc ggaccgaagc
     3301 gctcgcgcgc ctgagcgcac gatacctccc gttccagagc atgccgcgcc gatcggaaga
     3361 gccattctat ggtttggtgc gcaggctgac cgcccactcg ccagcgcggg tcatggcggc
     3421 gtggaccggc tctcctggcc aggccttcgg ctccgagtac gaacccagcc gcatccgggc
     3481 tcaccactct cgaatggctg cggtcttctt tggcgatgaa gcctgggccc ctctgatacc
     3541 gaactcgaac aaggtgagat tttcagtgcc ctccggcgac cgagtatcgg gcagcaacgg
     3601 ggtaccttcg gtcagcaatc aaacgccttc caaagacacg tcagtcttct tcgaggcgtt
     3661 gttcgaggtg ataggcgcgc ctcaactctc gcccaagccc gacacgctac tatggctcag
     3721 caacaaggct cgcgcgctgg tgacacacgt ggagcgcgct tcacgcgctg ctcgcaa
//
LOCUS       DASTKU010000495         3656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1879894, whole genome shotgun sequence.
ACCESSION   DASTKU010000495 DASTKU010000000
VERSION     DASTKU010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879894"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFQ61_17095"
     CDS             <1..190
                     /locus_tag="VFQ61_17095"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HET9956229.1"
                     /translation="PEPDGLQLVELRSLLRKLLRGRTVLGADLVEVQPDALANSTARA
                     ALQVAFELLDLLQPRASA"
     gene            187..1470
                     /locus_tag="VFQ61_17100"
     CDS             187..1470
                     /locus_tag="VFQ61_17100"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956230.1"
                     /translation="MTVTPPATAQRIAERSSGTVESSDQASRTPRSLHLSAHMSVRQA
                     VRNGSLTARDESTRLGVTLGLLEHYPPRRLQRIARRASRLLCFFDALWGTPDRAFECS
                     FEQAGRAFDVTGLKGTIIATAHFGAYRWLTLELLRHGVPVTLLVDAPFQQQLELDVQT
                     RVRAMYPAWATQDFATVNSADATALWQLVRALKRGRSVITFIDGNSGMEGKAAADGAL
                     ELSFLARRVRVRPGIAALSQAAEVPIVPLFSRVAPRQRAIFDVEAPILRAPEETRKAY
                     RERCMKELFSKLEREILARPGQWEEWWLLPHWLAPDCELPIRRRPRPEFQLSLPGLVR
                     TQLALPDGAIWVRSDGKVLDLGSGHSDSPQSGSSQNIPADPCLPSLLQAAECGAAARD
                     WLEAQPRPEAARLCLEREVRSGRVLLQRNRHATHP"
     gene            1454..1726
                     /locus_tag="VFQ61_17105"
     CDS             1454..1726
                     /locus_tag="VFQ61_17105"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET9956231.1"
                     /translation="MRLIPRSTPLPPPPAASKASGVPNAYVSIDRARCIGCAAGASHA
                     PELFEVSARGTRVKRQPESPEEVRQAETAALLCPVQAIDVHPTGAA"
     gene            1739..2674
                     /locus_tag="VFQ61_17110"
     CDS             1739..2674
                     /locus_tag="VFQ61_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9956232.1"
                     /translation="MLQTPAVTGPQGAVVSPRSTLQNRSEHTAQEVAATNDIVADALY
                     YRFFDQAERVRWRIADIPWYQLQADSVSEYWKQIVAEIARSELTTFDGTHQFMTRFGK
                     NADFSQWLAVWFYEETKHPQALMLWLDRVGVQLDHEFLVQGRSSPQFAASWLGTLTLN
                     VIAELLASEAYLVLSRYAPEPVLRHITQRIAADEARHAAHFFAYGQRLLARTAQPELE
                     LKNGLEVLYLWLNSETRLDHPVGAILNTIQATELFREGVGVQGFVTARLRQRVCQVIG
                     RMLGFRLESKADVVRAMRDLIRTRNASRPRGEGQP"
     gene            2671..3531
                     /locus_tag="VFQ61_17115"
     CDS             2671..3531
                     /locus_tag="VFQ61_17115"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HET9956233.1"
                     /translation="MNQQLAPLFGTDAHLPYGHRPAALGKSANEVEVDVYFADAREWD
                     TDAIGRASTLLAIPELERAKTYYFERDRQSYIAAHALLRSRLASRLGRPASEVVIGHT
                     PQGKPELAELTADSCHFSLSHSGPFVACAVARSPLGLDVERWRQSPPSGVPERCLSPA
                     EYSDVMCSPPYARGERFLRYWTLKEAYIKALGRDLRVPFSEIEFRLARGSGVQLVRTP
                     SLRDSSTSSSLTSLCFAAFSIPFEEARSRPRANHPHPAALAVLTERPIRIRCHSVDRT
                     LSVTNTTSDS"
BASE COUNT          646 a         1255 c         1119 g          636 t
ORIGIN      
        1 gcccgagccg gacggcctcc agctcgtgga gctacgctct ctattgcgca agttgctccg
       61 aggtcgaacc gtgctcggtg ccgatctggt cgaagttcag ccggacgcac tcgccaactc
      121 gaccgcgcgc gccgcgctgc aagtcgcgtt cgagctactc gacctcctac agccgcgagc
      181 aagcgcatga ccgtcacccc acccgcaacg gcgcagcgca tcgcagagcg aagctcgggc
      241 accgtggaat cctcggacca agcctccaga accccgcgct cgcttcatct gagcgcgcac
      301 atgagcgtcc gtcaggcagt ccggaatggg agcctgaccg ctcgggacga aagcactcga
      361 ttgggtgtga cgcttgggct actcgagcac tatccacctc ggcggctgca gcgaatcgca
      421 cgccgtgcca gccggctgct ttgctttttc gacgcgcttt ggggaactcc tgatcgagcc
      481 ttcgagtgta gcttcgagca ggccggcaga gcgttcgacg taactggcct caaagggacc
      541 atcatcgcca ccgcgcactt cggcgcgtac cgctggctca cgctggagct actgcgccac
      601 ggcgtgcccg tgaccttgct ggtcgacgca cctttccagc agcagctcga gctcgacgtg
      661 cagacgcggg tacgtgcgat gtatccggca tgggccacgc aggattttgc gaccgtgaac
      721 agcgcggatg cgaccgcgct ttggcagctg gtgcgcgcgc tgaaacgtgg tcgcagcgtg
      781 atcacgttca tcgacggcaa cagcgggatg gagggaaagg ccgccgccga cggcgcgctg
      841 gagctttcgt tccttgcccg acgggtacgc gtgcggcccg gcatcgccgc cctgtcccaa
      901 gcggccgagg tgcccatcgt gccgctcttc tcccgagtgg ccccgcggca acgcgccatc
      961 ttcgacgtgg aggcgccaat cctgcgcgcg ccggaagaaa cccggaaggc ttaccgggaa
     1021 cgttgtatga aagaactgtt cagcaagctc gaacgcgaga ttttggctcg cccggggcaa
     1081 tgggaggaat ggtggctgct gccacattgg ctcgcgcccg attgcgagtt gcccatccga
     1141 cgtcgaccac gacccgagtt ccaactcagc ttaccgggct tggtgagaac ccagctcgcc
     1201 ttgcccgatg gcgcaatctg ggtccgctcc gacggcaagg tgctggacct cggcagcggc
     1261 cactcggata gcccgcagag tggcagctcc caaaacatac ccgctgaccc ttgtctccca
     1321 agcctactcc aggccgctga atgcggcgcg gccgctcggg actggctcga agctcaacct
     1381 cgaccggaag ccgcgcgcct ttgtctcgaa cgcgaagtgc gcagtggccg cgtccttctc
     1441 caacgaaacc gtcatgcgac tcatccctag aagcacacct ctccctccgc cacccgccgc
     1501 atcgaaggca agcggcgttc caaacgctta cgtcagcatc gatcgtgcgc gctgcatcgg
     1561 ttgcgccgcc ggcgcttcgc atgcccccga gctgttcgaa gtgagcgcgc gcggcacgcg
     1621 agtcaaacgt caaccggaga gtcccgagga ggtgcggcag gccgaaacgg ccgccttgct
     1681 ctgccccgtc caagccatcg acgtgcatcc caccggggcc gcctgaggag ccgccaacat
     1741 gctgcaaaca cctgccgtca ccgggcctca gggcgcggtc gtttcacctc gatccacgtt
     1801 gcaaaatcgc agtgagcaca ccgcccaaga agtcgcggcc acgaacgata tcgtcgccga
     1861 cgcgctctac taccgcttct tcgatcaagc agagcgcgtc cgctggcgga tcgcagacat
     1921 tccttggtac cagctgcaag ctgactccgt ctcggagtac tggaagcaga tcgtcgcgga
     1981 gattgcccgc tccgagctca ccaccttcga cggcacccac cagttcatga ctcgcttcgg
     2041 caagaacgcc gacttctcgc agtggctggc ggtctggttt tacgaggaga ccaagcatcc
     2101 gcaagctctg atgctgtggc tcgatcgagt cggcgtgcag ctcgaccacg agttcttggt
     2161 tcaaggccgt agtagcccgc aattcgcggc atcctggctt ggcacgctga cactcaacgt
     2221 catcgccgag ctcctcgcca gtgaggcgta cctggtgttg tcccgctatg cgcccgagcc
     2281 ggtgctgcga cacatcaccc agcgcatcgc cgccgacgag gcgcggcatg ccgcgcactt
     2341 cttcgcttac ggccagcgtc tgttggcccg taccgcgcag ccagagctgg agctgaagaa
     2401 cgggctcgag gtcttgtatc tttggctgaa cagcgaaact cgactcgacc atccagtagg
     2461 tgcgatcttg aatacgattc aagccacgga gctgttccgt gagggagtgg gagttcaagg
     2521 cttcgtcacc gcgcgcttac gacagcgcgt gtgccaggtc atcggacgca tgctgggctt
     2581 tcgcctggaa agcaaagccg acgtggtgcg cgccatgcgc gacctcattc gcacgcgcaa
     2641 cgctagccgc ccgcgcggcg aagggcagcc atgaaccagc agctcgcgcc gcttttcgga
     2701 acggacgcgc acctgccgta cggccatcgg ccggctgctc tcggtaaaag cgcgaacgaa
     2761 gttgaggtcg acgtctattt cgcggacgcg cgcgagtggg acaccgacgc catcggccga
     2821 gccagcacgc tgctcgcaat acccgagctc gagcgagcaa agacgtacta cttcgagcgc
     2881 gatcggcaga gttacattgc cgcccacgcg ctcttgcgca gccgcctcgc atcgcggctc
     2941 gggcgacccg cgagcgaggt cgtgattggc cacactcctc agggcaaacc cgagctcgcg
     3001 gagcttacgg cggactcgtg tcacttcagc ctgagccatt cgggcccgtt cgtcgcctgt
     3061 gccgtcgcgc gttccccgct gggcttggac gtcgagcgct ggcgccaaag cccaccgtcc
     3121 ggggtgcccg aacgttgcct gagcccggcc gaatacagcg acgtgatgtg cagcccgccg
     3181 tacgcgcgcg gcgaacgctt cttacgctat tggacgctga aagaggccta catcaaggcc
     3241 ctcgggcgcg acttacgcgt gccattctcc gaaatcgagt tccggctggc gcgcggatcg
     3301 ggggttcagc tcgtccgtac gccgtcgctc cgcgacagca gtacgagctc ttccctcacc
     3361 tcgctatgtt ttgccgcgtt ctcaattccg ttcgaggagg ctcggtcccg tcctcgcgcc
     3421 aaccacccgc acccggccgc gttagcagtc ctaaccgagc gccccattcg aattcgatgt
     3481 cactccgtcg atcgaacgct gagcgtgacg aacaccacgt ctgactcgtg agcgctgccg
     3541 gtcctcgtca gttgccggta tcggggcgat gttggatggg agaaagcggc ggcaactcga
     3601 gggccactcg ccaatgcccg tcctcgagcg cgcaacggac cgtgtgcgag tgtgct
//
LOCUS       DASTKU010000496         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_95132,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000496 DASTKU010000000
VERSION     DASTKU010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95132"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFQ61_17120"
     CDS             complement(<1..456)
                     /locus_tag="VFQ61_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956234.1"
                     /translation="MLAPMFRVRSRSQRVLSALLVLPALLGTLLASVSAARAADDPGW
                     FDPAASQGSNVQGVQGGAVQGSSTSGAPAAASNQAPQEMPSLAPSPLITERRPTSSSF
                     SGQASAESSVQVSAQDRDPRALSDFRPSLDPYGSWVNHPSYGTVWIPSRA"
     gene            572..1762
                     /gene="glp"
                     /locus_tag="VFQ61_17125"
     CDS             572..1762
                     /gene="glp"
                     /locus_tag="VFQ61_17125"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET9956235.1"
                     /translation="MLDFHEAQRRVVAAAQRLGTETLPVGATAGRVLAEPLRGATPLP
                     AFDYSAMDGYAVRCADFAESGPWESQVAGESRAGCAPGTLVPGTCIRIFTGAPIPAGA
                     DAIVIQEDTDREGLLARFRVKPKINAHIRRAGEDLASGAIALDAGTRLSAFHLGLIAA
                     LDRGRVEVSQAPRVIVAPTGDELREPGSASRPGSIPESNGVAIAALAERAGAKVQRVD
                     VLRDDRAETTERLKNLLMQCDVLITIGGVSVGDHDVVRDALQAAGATLEFWKIAIRPG
                     KPFTFGRAGSTLILGLPGNPVSAQVTFSLLGIPLLRAMQGDRQALPPTLRVKLTEAWT
                     QKPGRMGIHRARLDGDRATLERGGSSGSVLSMARADALVFVPAEVEHCPAGTELDAVD
                     LRQL"
     gene            1770..2354
                     /locus_tag="VFQ61_17130"
     CDS             1770..2354
                     /locus_tag="VFQ61_17130"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HET9956236.1"
                     /translation="MTLVTRLSVGLFVGGRGLRMGGVPKGMLKLRSGQTLIERLLGEV
                     SRAYPRAQVYLVGAATEYQHLGLRALTDDPPGIGPLGGLRALLREAETAGATHVLALA
                     CDLPYVASPLLARLAEEAPAALALAAKQDGYWQPLCARYSVALEAAADELVRQRRHSL
                     QALFEGLGESAVELGMSPSELTELKDWDRPEDLD"
     gene            complement(2320..>2648)
                     /locus_tag="VFQ61_17135"
     CDS             complement(2320..>2648)
                     /locus_tag="VFQ61_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET9956237.1"
                     /translation="VALEYEAYATMAIAEFERIAQELEREFPAVRLAVLHRTGRLNVG
                     QVAVLCAASAAHREAAFDAARALIDRTKARVPIWKREHGEQGPYWVGWEDARTASPSL
                     PGDPSP"
BASE COUNT          471 a          839 c          895 g          443 t
ORIGIN      
        1 ggctcgcgaa gggatccaga cagtgccgta cgaggggtga ttcacccagg atccgtaagg
       61 atcgagcgag ggtcgaaaat cagacagcgc gcgcgggtca cgatcttggg cggagacctg
      121 cacggaactt tctgcgctcg cctgacccga gaaggagcta gacgtggggc ggcgctccgt
      181 aattaggggc gacggcgcga gcgacggcat ctcttgaggg gcttgattcg aggccgcggc
      241 aggcgcaccg ctcgtgctgc ttccctgaac ggcaccgcct tggaccccct gtacattgct
      301 gccctggctc gcagctggat cgaaccagcc gggatcatct gcagcgcgcg cggcgctcac
      361 actcgccagc aacgtgccga ggagcgcagg cagaaccaac aaagccgaga gcaccctttg
      421 cgagcgtgag cgaacccgaa acatgggagc aagcatgaga ccttccgtgc agcgtggcaa
      481 acgccgaact gttcgacgca gcccctgtcc agaagcttca ctgaattcga ccgctccggc
      541 gcgatgaaga cctttggtat ggtgtgcggc gatgctcgat tttcatgaag ctcagcggcg
      601 cgtcgtggct gccgcccaac gtctgggcac agaaactttg ccggtggggg ctaccgccgg
      661 acgcgtgctc gcggaacctc tgcgcggcgc gacacccctg cctgctttcg actacagcgc
      721 gatggacggc tatgcggtgc gctgcgcaga ttttgctgaa agtgggcctt gggagagcca
      781 ggtcgctggg gagagccgcg ccggctgcgc accaggaact ctggttccgg gtacctgtat
      841 ccggatcttc acgggtgcac caatccccgc gggcgccgat gcgattgtga ttcaggagga
      901 tacagatcgc gaagggttgc tcgcacgctt ccgggtgaag ccgaaaatca acgcgcatat
      961 ccggcgcgcg ggggaggatc tagcgtcggg tgcgatcgcg ctggacgcag ggacacgtct
     1021 ttcggccttt cacttgggcc taatcgcggc gttggaccgg ggccgcgtcg aagtgagcca
     1081 agcgccgcgc gtgatcgtcg cgcccaccgg agatgagctc cgcgagcctg gtagcgcgtc
     1141 ccgccctggc tcgattcccg agtccaacgg cgtggccatc gccgcgttgg cagagcgggc
     1201 cggcgccaaa gtgcaacgag tcgacgtgct ccgcgacgac cgtgcggaaa ccaccgagcg
     1261 gctgaagaac ctgctgatgc aatgcgacgt gttgatcacg atcggcggag ttagtgtcgg
     1321 cgatcacgac gtcgtgaggg atgccttaca ggccgccggc gcgacgctgg aattctggaa
     1381 aatagcaatc cgtcccggta agccttttac attcggacgc gcgggctcca cgctgatcct
     1441 aggtttgcct ggcaaccccg tatcggcaca ggtgaccttc tccttgctcg gcatcccgct
     1501 gcttcgagcc atgcagggcg atagacaggc cctgccgccc acgctgcgtg tgaagctgac
     1561 cgaagcttgg acccaaaaac ccggacgcat gggcattcac cgggcgaggt tagacgggga
     1621 ccgcgccacg ctagaacggg gcggaagctc aggctcggtg ctctcgatgg cgcgcgcaga
     1681 cgccctggtg ttcgtgccgg ccgaagtcga gcattgccca gccggaaccg agctggacgc
     1741 cgtcgacttg aggcaactct aatcggagca tgacgttggt gacccggctc agcgtgggct
     1801 tgttcgttgg cggccgtgga ctgcgcatgg gcggcgtgcc caagggcatg ctgaaactcc
     1861 gaagcgggca aaccttgatt gagcggctac tcggagaggt gagccgtgcg tacccaagag
     1921 cacaggttta cctggtgggc gccgcgacgg agtaccagca cctggggctg cgagcgctca
     1981 ccgacgatcc acccgggatc gggcccctcg gtgggttgcg cgcgttgctg cgggaagccg
     2041 agaccgcggg cgccacgcac gtgctggctc tggcgtgcga cttaccctac gtggcgagcc
     2101 cgttgctcgc gcggctggcc gaggaagctc ccgcagcttt ggccctcgcc gcgaagcaag
     2161 acggctactg gcaacctctg tgcgcacgtt actctgttgc gctggaggcc gccgcggacg
     2221 agctcgtgcg gcagcgacgg cactcgctac aagcgctttt cgaggggctc ggtgaaagcg
     2281 cggtggagct gggcatgagc ccgagcgagc tcaccgagct caaggactgg gatcgcccgg
     2341 aagacttgga ctagccgtcc gtgcgtcttc ccaaccgacc cagtacgggc cttgttcgcc
     2401 gtgctctcgt ttccaaatcg gcacccgcgc tttggtccga tcaatcaacg cgcgggcggc
     2461 atcgaaggct gcctcgcggt gggctgcact agcggcacag agcaccgcaa cctggcccac
     2521 gttcaaacgc ccggtgcggt gcagcaccgc gagccgcacc gcgggaaact cccgctcgag
     2581 ttcttgagcg atccgctcga actcggcgat cgccatcgtc gcgtaggcct catactcgag
     2641 cgccacca
//
LOCUS       DASTKU010000497         6032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_762992, whole genome shotgun sequence.
ACCESSION   DASTKU010000497 DASTKU010000000
VERSION     DASTKU010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762992"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            739..1806
                     /locus_tag="VFQ61_17140"
     CDS             739..1806
                     /locus_tag="VFQ61_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956238.1"
                     /translation="MRRNTVGILTLACFASSSAAGADTQAGGTTTAPTIELNWSGPGP
                     ERQCMGGPALEREVNAYLRREAITEPPGELALSVLVQERPGRQWSATIYLRDQREGRV
                     IGERELVVDGPLCSSLDEPLKLAVALLVDADLLPPESAAPPTPQPSNPPPAPEDEQQP
                     EDESEEGALPTGVPEFDAPAPHRSRVGLELAALAGSTALPELGVGAQLGLHWSALRWL
                     ALRAHVAAFAPQTVTLGADAEVRSSLWYSGASICPRAFVNEGLHLLGCAALEGGSLSL
                     SSRGLESGFRSRRAVWLASLGGRVQWQLVDSLALFGQLSVAWPLLRQRFWYEEVGKRQ
                     DLFSLSAPTAALGVGLTLWLN"
     gene            1873..2409
                     /locus_tag="VFQ61_17145"
     CDS             1873..2409
                     /locus_tag="VFQ61_17145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9956239.1"
                     /translation="MDVGPARKLPVAPEISFAEIFRQQARYLWRALLGLGVRPADVDD
                     VCQEVFLVVHRRLPEFDGRALRSWLYAICLRVASDYRRSARVRREVPLADLPEAVPNH
                     AALGEVHVRELWHTMLRGLDRLDEEKRAAFVLYEIEELTVREVAEVMGCPLQTAYSRL
                     GAAREQMQAFFASEGRTP"
     gene            2406..3398
                     /locus_tag="VFQ61_17150"
     CDS             2406..3398
                     /locus_tag="VFQ61_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956240.1"
                     /translation="MNDPKRLIHESNALGVALAGARRQVPSARQLSELAQRLSQAGAP
                     VGFEHGSFEQGSSENGSLRPRSSEPDAAGRGTNQPEAYDSATRPTAVEKSFPWGKWAL
                     GVVGGAGTLAALWVGTGRSPIGNPVSGAAKTVVSAAPSATELPRGVPEDRLPNPELEN
                     PTQASGKAGSSVPLRSVQRDRMPEASTPTQVEGPATTHTAPSSSNSAPPPRAAEPANS
                     RARAVPSVNVAPGRGFEGSEPTGKEAVLSEIELLKRARAELSGDPLRAYVLTETCRTQ
                     YPRGAFAQEREFIAITALSKVGREQEARSRASLFRSHFPRSAYLKQLAPLFEAP"
     gene            3395..5329
                     /locus_tag="VFQ61_17155"
     CDS             3395..5329
                     /locus_tag="VFQ61_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956241.1"
                     /translation="MKPTLRACLVALSASACGPTESVYLGSLPANGGRGGGTQAGAGM
                     ASGGSSGVGEWICNSEPCATHSGSRVDSSSGVPADAPERMAAAPPAPSGANELAWLYP
                     NHESRIPANLGPVRFEWLADETDRLFQVRYSGPRTEVMVFTETTHYEPSPELWDWIAE
                     SNIGGAVTIQLSALTNSGERKEASAVQIEIGIPLEEGAVYLWSTAARGVLRAAVTSTH
                     PERVEPSAESQQDIDTLAGAMPAPADDAGAACAGCHSLSRDGSRLALDLGNGRLRVAD
                     VRGLRAYSGAFSGTGGEGGTPIGKSEKVGPSNPAVWSTFSPDSTRLLAASGGKLQLFD
                     LRDTAQPSQPVASVPVPEKRVPSHPDWSPVGDRIAFTLAEKGQGRAVERGAIAVVDYL
                     AEMLDAPFGAVEIVVPSQGVQENNLFPSFSPDGQFLAYVTLSGNSENPQRASVRMVRL
                     SDRKTFELERLNRRLGTSDSDLAFGNSMPTWVRSADRETTWLAFSSLRPYASVRPLDA
                     KRDQIWLAAIDMGAAARAQPTELVDPSWAAFWAPFQLLEHGNHRALFAPPSLVPPSPP
                     ASCSCIELCADLVDNDCDGSVDEEPCHATCEARERCDDGTDDDCDCVVDDCSLELCSD
                     GLDNDGDGFADAADLSCPSP"
     gene            complement(5343..>6032)
                     /locus_tag="VFQ61_17160"
     CDS             complement(5343..>6032)
                     /locus_tag="VFQ61_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956242.1"
                     /translation="AGPGKNGHTFDDLVGSDHVELMLTDGAGATVMDFKLDYITGASS
                     TKCGYTNLGISGGEGKIITGNASAVVGATSSLDRNLNGCGYCLTEDSPATDASYTPNS
                     QYPNWDFRVVYEVWVDLDAFGSAGFGQAYIESVHASPSKLDNNTVTVEPGPCPPSWDS
                     PYCPPGSPTGAQCQTETSCPPNQQLYIQTEGKSVCTPIPFANYPNRAPCPSGYQLDAQ
                     TEGRYCVPVTG"
BASE COUNT         1013 a         1896 c         2003 g         1120 t
ORIGIN      
        1 cccgagctcg cggccgcaac caatagagac caggtgaccg tcttgatgac gttccagtga
       61 ttcactgctt caactccttg cgatcctaga tgcgcggttc aaccgtgtag cgtctgccgg
      121 gaaggagttc ggcttcacgg acgcggcgtg aaacgcaaac gagtacccgg aaagcctgcc
      181 cgcctccagt cacactcacg ggcattagag gcgcgacccc gctgcacctt cgcaaaaatg
      241 atcctgctcc cgacttttct agcgcaactg ctcctgggtg gagtccgagc tcgtggtccg
      301 gcggttcaca cgcatgggtg atgtgcgtac gcgggcgagc tgaaaaggag cagtcgttgc
      361 ccaatctgag agcgctcggc atccaagcga acacgcagtc tgggcgcggg tggcctcgcc
      421 gacagcaaag ctcggacacg cttcggatcc tggtttgtcg gcctcgatgt aaaggggcgc
      481 gactgctcgt cgaggaacgg cgcgcgcgca gtcccgttga acccccgcgg cccgcgctac
      541 cgcgcccgcc gcacgcggta acccggacgt ctccatcctc cgcggcgggt ccgtgcggtg
      601 tctcggccta atttccactc tttttcaagc ggcgacagga tcgaagccgt acgctagtct
      661 gggctggatg cccgttcgcc cgcgccaccc ccgcctccca tggcggcaac agcaccccgc
      721 tccaggagct gccccgtcgt gaggcgcaac accgtgggga tcctgacgct cgcgtgcttt
      781 gcttcctctt cagcggcagg ggctgatacg caggccgggg gaacgacgac ggccccgacc
      841 atcgagctca attggagtgg accaggtccc gagcgccagt gcatgggtgg accggcactg
      901 gagcgtgaag tgaacgccta cttgaggcgc gaggcgataa ccgaaccccc cggtgagctc
      961 gcgctctcgg tgctcgttca ggagcgacca ggtcgtcagt ggtcggccac aatttacctg
     1021 cgtgaccagc gcgagggccg tgtgatcgga gagcgtgagt tggtcgtgga tgggcctctc
     1081 tgctcgagct tggatgaacc tctgaagctc gcggtggccc tgctcgtgga cgccgacttg
     1141 cttccgcccg agagcgcggc gccaccgact ccgcagccca gcaatccgcc cccagcaccc
     1201 gaggatgagc agcagcccga agacgagtcg gaggaaggag cacttccgac cggcgttccg
     1261 gagttcgacg ctcccgctcc gcaccgttca cgtgtggggt tggagcttgc ggcattggcg
     1321 ggttcgacgg ccttgcccga gctgggtgtc ggagcccagc tcggtttgca ttggtcggcg
     1381 ttgcgctggc tcgcgctgcg cgcgcacgtg gccgcttttg cgcctcaaac cgtgaccctc
     1441 ggcgcggacg ccgaggtccg ctcttcgctc tggtactcgg gggcttctat ttgtccacgg
     1501 gcgttcgtga atgaggggct gcacctgctc gggtgtgcgg cgctcgaggg cggctccctg
     1561 tccttgagta gtcgcggttt ggagtcgggc tttcgctctc gacgcgcggt ttggctggcc
     1621 tccttgggcg gccgcgtcca gtggcagttg gtcgactcat tggctctgtt cggtcagctc
     1681 tcggtggctt ggccgctact gcggcagcgc ttttggtacg aggaagtcgg aaaacgccag
     1741 gaccttttct cgctgagcgc gcccacagcg gcgctcgggg tcggactgac gctgtggctg
     1801 aactgatgcg cgcgcagcac caaattctcc gcggccgctc agaaaaaggg cgcgctgcta
     1861 ccatagaggg gcgtggacgt cggtccagct cgtaagttgc cggtggctcc cgagatatcc
     1921 tttgcggaga tttttcggca gcaagcccgt tatttgtggc gcgcgctgct gggtctcggt
     1981 gtgagacccg ccgacgtgga tgatgtttgc caggaagtat tcctggtcgt gcaccgccgg
     2041 cttccggagt tcgacggtcg cgcgctgcga agctggctct atgcgatctg cctacgcgtt
     2101 gcttcggact atcggcgcag cgcgcgagtg cgtcgcgaag tcccgttggc cgatttaccc
     2161 gaagccgtcc ccaatcacgc cgccctcggg gaagtccatg tccgagagct ctggcacacg
     2221 atgctgcgcg ggctcgaccg cctagacgaa gagaagcgcg ccgcgttcgt tctttatgaa
     2281 atcgaagagc ttacggtgcg cgaggtcgcg gaagtgatgg gctgccccct gcagacggcc
     2341 tactctcgcc taggcgccgc gcgcgaacag atgcaggctt tctttgctag tgaagggaga
     2401 accccgtgaa cgatcccaag cgcctcatcc acgagagcaa cgccctcggc gtcgctctgg
     2461 cgggcgctcg acgccaggtc ccttctgcga ggcagctctc ggaactcgcg cagcggttga
     2521 gccaagcggg agcgccagtg gggttcgagc acggttcgtt cgagcagggc tcgagcgaaa
     2581 acggctcgtt gagacctcgt tcgagcgagc ccgacgcagc gggacgcgga acgaaccagc
     2641 ccgaagctta tgactccgcg acccgcccca cagccgtgga gaagtccttt ccctggggga
     2701 agtgggccct cggggtggtc ggcggagcgg gcactctagc agcgctttgg gttggaacgg
     2761 ggcgttcgcc gattggtaac cccgtctcgg gtgcagcgaa aacggtcgtg tccgccgctc
     2821 cttccgcaac cgagctcccg cgtggagtcc ctgaagatcg tctacctaat cctgagctcg
     2881 agaacccgac tcaggcaagc gggaaggctg gcagctcggt gccgctccga agcgtacaac
     2941 gggaccgcat gcccgaggct tccaccccga cccaagtcga aggtccagct acgacgcata
     3001 cggcgccgtc ctcttcgaac tcggcgccgc cgcctcgcgc tgcggagcct gcaaactcga
     3061 gagcgcgtgc agttccgtcc gtgaacgtgg cgcccggaag aggcttcgag ggttcggagc
     3121 caactggaaa agaagcggtg ttgtcggaga tcgagctgct caagcgtgcg cgcgcagagc
     3181 tgagcgggga tccgctgcgg gcctacgttt tgaccgaaac gtgccgtacg cagtatccgc
     3241 gaggcgcgtt cgctcaggag cgtgagttca tcgcgattac ggcgctctcg aaggtggggc
     3301 gagagcaaga ggcgaggtct cgcgccagct tgtttcgttc tcacttccct cggtcggcgt
     3361 atctgaaaca actcgctccg ctgttcgagg cgccgtgaag cccacgctcc gcgcgtgcct
     3421 tgtcgctctc tcggcgagcg cgtgcggacc gacggagagc gtctatctcg gttccctgcc
     3481 agcgaatggc gggcgcggag gcggaaccca ggcgggcgcc ggtatggcga gcggcggatc
     3541 gagcggggtg ggcgagtgga tctgcaactc tgaaccttgc gcgacccact ccggtagccg
     3601 agtggactcg agttccggag taccagcgga cgccccggag cgcatggcgg ccgcgccacc
     3661 cgcgccgtcc ggggcaaacg aattggcctg gctctatccc aatcacgaga gccgcatccc
     3721 tgccaatctt ggccccgttc gcttcgagtg gttagccgat gaaacagatc ggttgttcca
     3781 agttcgctac tccggcccgc gcacggaagt gatggtcttt accgagacca ctcactacga
     3841 gccgagtccc gagctttggg attggattgc tgaatccaat atcggaggtg cggtgaccat
     3901 ccagctcagc gcgctaacga actcagggga gcgcaaagag gcctccgcgg tccagatcga
     3961 gatcggcatt ccgctcgaag agggggccgt ctacctttgg tcgaccgccg cacgcggggt
     4021 gcttcgggcg gcggtgactt cgacgcatcc agagcgagtc gagccgtccg cggagtccca
     4081 acaggacatc gataccttgg cgggcgcgat gccggccccc gcggatgacg cgggcgcagc
     4141 gtgtgcgggt tgtcacagcc tatctcgcga cggttcgcgc ctggcgctgg acctcggcaa
     4201 tggtcggctg cgtgttgccg acgtccgcgg gctgagggcc tattccgggg cgttctcagg
     4261 gacgggcggc gagggaggaa ccccaatcgg aaagtcggag aaggtagggc cgagtaaccc
     4321 ggcggtgtgg tccacgttct cgcccgattc gacgcgtctg ctcgccgcca gcggcggcaa
     4381 gctgcagctg ttcgacttac gagacacggc tcagccgagc caacccgtcg cctcggttcc
     4441 cgtcccggag aagagggtcc ccagccaccc agactggtcg cctgtcggcg atcgcatcgc
     4501 gttcacgctc gccgagaagg ggcaaggccg cgccgtggag cgcggggcga tcgcggtggt
     4561 cgactacttg gcagaaatgt tggacgcgcc gtttggtgcg gtggagatcg tcgtcccgag
     4621 ccaaggcgtt caagaaaaca atctgtttcc gtcctttagt cccgacgggc agttccttgc
     4681 ttacgtgact ctgagtggca attccgaaaa cccgcagcgc gcgagtgttc gtatggtgcg
     4741 gctcagtgat cggaagacct tcgagctcga gcgtctgaac cgtagactag ggacatccga
     4801 ttcggatttg gcgttcggaa acagcatgcc gacttgggtt cgaagcgcag accgcgagac
     4861 cacttggttg gcattttcga gtctgcgccc ctacgcgagc gtgcgccctc tggacgcgaa
     4921 gcgtgaccag atttggctgg ccgcgatcga catgggtgcg gccgcgcgcg cgcaaccgac
     4981 ggagctcgtc gatccatcgt gggctgcttt ttgggcgccc tttcagctgc tggagcacgg
     5041 aaaccaccgt gccctgttcg cgccgcccag tctcgtgccg ccgagccccc ccgcgagctg
     5101 tagttgcatc gagctttgcg cagatctggt cgacaacgac tgtgacggca gtgtcgatga
     5161 agagccgtgc cacgccactt gcgaagcgcg cgagcgctgc gacgacggaa ccgacgacga
     5221 ctgcgattgc gtggtcgacg attgcagctt ggagctctgc agcgatgggc tagacaacga
     5281 cggcgacggg ttcgccgacg ccgccgatct gtcctgccca tcgccgtgag gctcagtccc
     5341 ggttagccag tgacgggaac gcagtaacgc ccttcggttt gcgcgtctag ctgatacccc
     5401 gacgggcaag gcgcccggtt gggataattg gcaaacggga ttggcgtaca gacactcttc
     5461 ccctccgtct gaatatagag ctgctggtta ggcgggcatg aagtctcggt ctgacattgg
     5521 gcgccggtag gacttcccgg tggacaatag ggcgaatccc aggaaggcgg gcaggggcct
     5581 ggctctaccg taacggtgtt gttgtcgagt ttggaagggg aggcgtgtac ggattcgata
     5641 taggcctgcc caaaacccgc gctcccgaaa gcatccagat ccacccagac ctcgtacacc
     5701 acgcggaagt cccaattggg atactgcgag ttgggggtgt agcttgcgtc ggtcgcgggc
     5761 gagtcttcgg tcaggcagta tccgcagcca ttcaaatttc ggtcgagcga gctcgtcgcg
     5821 ccgaccacgg cgctggcatt gcctgtgatg atcttcccct caccgccaga gattcccaga
     5881 ttcgtatagc cacacttcgt gctgctcgca cccgtaatgt agtcgagctt gaagtccatg
     5941 acggttgccc ctgcgccgtc agtcagcatc aattccacgt gatcgctgcc gaccaaatcg
     6001 tcgaaggtgt gtccgttctt gcccgggccc gc
//
LOCUS       DASTKU010000498        14303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_317754, whole genome shotgun sequence.
ACCESSION   DASTKU010000498 DASTKU010000000
VERSION     DASTKU010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14303
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317754"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFQ61_17165"
     CDS             <1..187
                     /locus_tag="VFQ61_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956243.1"
                     /translation="APRLALASDQAVISPRDGGSPSRKSGRLTGSSATLCAGERQPHR
                     SDAGETASQAPVSRPEH"
     gene            380..1153
                     /locus_tag="VFQ61_17170"
     CDS             380..1153
                     /locus_tag="VFQ61_17170"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9956244.1"
                     /translation="MFAKRVSAAHSSASAGPMGLEPGTQLGNHRVLFCTARGGMASVW
                     PALQLGPRGFSKLVALKAILPDLVASPEFEALFIEEARIAARIRHPNVCEVFELIEEN
                     GVLALSMEWIDGDALGRVLETPPQPLNPRIAAYIVAQAAAGLHAAHELRDDAGDAMNV
                     VHRDVSSQNILISKDGHVKISDFGIAKAFGSTRDATQAGRVRGKLAYMSPEQASAAAT
                     DRRSDVFSLGVVLYQITIGQIPFRQADDTTRTHLTRLLE"
     gene            1211..1906
                     /locus_tag="VFQ61_17175"
     CDS             1211..1906
                     /locus_tag="VFQ61_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956245.1"
                     /translation="MSRALSRDPAQRYPSADAMRLALEGWLARSGPPVNERDLARVVR
                     ERRGGEIAARELKIRTLSEIACLPRGAHTGKTLEPIALGPEPVTWTERLAPRPRASRI
                     WLLAGGAALFTLSLVIRARIVGSEAESAHAGTTSIRSDAAPPARDVRPAPPLALSAVP
                     PPERNVTPPAGTEAEKPRSREAEKPSLKRAPQAVKASAATLRAPMPPKRESARPTPNP
                     RTKPTLGPLEREL"
     gene            1903..2961
                     /locus_tag="VFQ61_17180"
     CDS             1903..2961
                     /locus_tag="VFQ61_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956246.1"
                     /translation="MTWVLHIEWIWRESLKATLWLTALGVGVTGLAFSPGVAVAQSSD
                     IESARRSFREGNDLLERNEYAAALAKFERAHESWANPKIRLNIATTLRALGRAADAGN
                     AYALYLEEVGADSGKREEVERVLDELRRSLARVQVAEYGVSRAELDGRKISNSTRGYF
                     VEPGRHSLILTGRAGTSRREVELRAGELYLIEPEPNQPETPAATLPARAEMPTRQPAS
                     GLSDVNTPSKLFVLSRVDIEGSGRGAVAALGFGYELDPHVRANLGALIGTNQGGWLGI
                     HVNPSVGLLRPMLGVSVPAFVVEGIHPGIAGELGLQLALDSRWYPFARASVVHFPGLA
                     TNYVSTLFVPAAGMEVRL"
     gene            2964..4553
                     /locus_tag="VFQ61_17185"
     CDS             2964..4553
                     /locus_tag="VFQ61_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HET9956247.1"
                     /translation="MASLALRGVALGASWVAPLAVNLACSASDAVPPELARRTAALEA
                     VQFTPISNSTLPNARSHAGFVYDPAIDGSVAFGGHLPSDATLQGLRDTWVWDGVRWNS
                     PPAGYPQRSFVSGIYDPSSGKLLSYGGYAATAYNNDTWEFDGLNWSSRFNLLTPGKRS
                     SYGMAHDSSRGVTVLFGGYNGQWQGDIWEWDGTLWTERCTNSPCSTSPKPSARANATF
                     VYDEARKVTLLFGGYGPGGSRSDTWLWNGSQFVLVKTAIMPSPRDSAAATYDPVTQRV
                     LLFGGIQSDGVEQNDLWAWDGVQWQSIAQSTPATPRQGAGLAWDSKRRRGVLVGGEFL
                     GKPVEPWEFTVSGNRCSSDLDCHQSSCLSGICASVSTNSGSGGSADPGAGGESANGGA
                     AGDGGAGGATNESRGGNPTGAGGSLATSPTSEGATSGRAGSGGSGGSGSSGAGTQGGH
                     AGSGSPMVQAGADSGGAGRDTGTEGTLAVVPSAAEDAVNFYACSVIGVAHGSPLDSRV
                     CLSALAVGLLLVERRRRRHKP"
     gene            complement(4829..6169)
                     /locus_tag="VFQ61_17190"
     CDS             complement(4829..6169)
                     /locus_tag="VFQ61_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956248.1"
                     /translation="MKGKKLHSFESTARLVRDRSRLGVPWVATTLVLFANAGCSASGS
                     DPFGDAAGVPGFGGSSVQAGRSGSSGGVVGNVGSGGTNGGSLTGSGGSIAALGGTSAS
                     AGSGGRGAQGGIANGGSTIGGSSGVAGWFGNGGRAAGGAKSTGGASGALGGATGQSGS
                     GAMGGSGAGGTPTSAGGGSIAMGGASTTGGASSGSQPQLPPISGTCPNFATGTTSIGG
                     LSGISLQVGAKKEGTGSLIFYWHGTGSSASEVNIMMPAAVRQEILNQGGIIVSFQSSL
                     RTGGDCSGTSTFSKDDFKIADLIAACAVRDHNIDPRRIYTTGCSAGGLQAGCMGALRS
                     NYIAAVVPNSGGEVTRQPIQNPATTPAVMTMHGGSSDVVVVAFSTTSATYDSHMKSAG
                     SFVVNCDHGGGHCGAPSALYSAGWEFMKAHPFGVEPEPYTSGLPSSFPSYCKVY"
     gene            6605..7753
                     /locus_tag="VFQ61_17195"
     CDS             6605..7753
                     /locus_tag="VFQ61_17195"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET9956249.1"
                     /translation="MKVVIIGASVAGSATALALARAGHDVQLFERGETAETGAGFVLW
                     PNATFVLDRLQILDAIGELSARPAAMTRFDLAGQALGGWDLLALERKMGYPSLSILRS
                     DLLRILHRALRRQGVEVQTGCRAIELLDSPGVQFEGGRTITADLVVGADGRRHSVTRR
                     YVRPEHAAIYQGFVNWVGVSPVRLSRPIVHDYAAAERRFGIVPVAGEKVYWAGAMRRD
                     SAPAPNETLSRAELLELFTGWPPVVREVIERSEKGAFKTIGIYDVEPNLAWHRGNVLL
                     IGDAAHAACPTSGQGVSQALEDAWHLRDCLPKRATRVSDALTEFTRLRFEKTRAITQS
                     GRALARELFRPQSAGAAAHRPSGAALDLASLASFWGAGLPLQKSGSTP"
     gene            7974..8828
                     /locus_tag="VFQ61_17200"
     CDS             7974..8828
                     /locus_tag="VFQ61_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET9956250.1"
                     /translation="MAYLLPTEFVTKMVDAGESKIFMSTRDTVIRGTMAGALLALSAV
                     FAVSVTVQTNSPLLGAVLFPVGFCMLYLLGFDLLTGVFMLSPLALIDKRPGVTMGGVL
                     RNWGLVFLGNFAGALLVALMMSYVFTYGFSTDPGPVGAKIAGIGKARTLGYATYGMAG
                     WITIFVRGMLCNWMVSTGVVGAMISTHVSGKVIAMWMPIMLFFYMTFEHSVVNMFLFP
                     SALIMGGQFSVADYFVWNEIPTVLGNLVGGITFTGLTLYSTHVRTAPKRKAAVSAAPA
                     GVAGRAQA"
     gene            8881..10614
                     /locus_tag="VFQ61_17205"
     CDS             8881..10614
                     /locus_tag="VFQ61_17205"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9956251.1"
                     /translation="MHTTLQVTLGQASDKGRKPTNQDFHAASIPREPLATLKGICVAI
                     ADGISTSEVSAEASEAAIRSFIEDYYCTSEAWSVKTSVERVMSAANSWLHSQTLRSPY
                     RYERDHGYVCTFSAVVFKAATAHVFNVGDSRVYRLTETALEQLTEDHRVLRAPGESYL
                     ARALGAAAHLELDYRAVPLEPGSIFVLATDGVFEHIAPDRLRECVADHVDDLDEAARQ
                     LIKLAYENGSPDNLTAQIVRVDAVPATTSAELFHELSDLPLPPLLEERQRFDGYTIVR
                     ELHASHRSHVYLALDDETSERVVLKIPAIDLREDSALLERFLLEEWIARRIRNPHVLA
                     PRTPRKRNFVYLVTEYVEGQTLSQWMRDHPRPNIEQVRSIVEQVAHGLNAFHKHEMIH
                     QDLRPDNILLDPTGTVKIIDFGSVHVAGVAEGVTAERTEIAGTLQYAAPEYFLGEPGS
                     NRSDLFSLGVIAYQMLAGRLPYGTEVSKCRTRLARAQLRYTSVLSDDREIPAFVDEAL
                     RKAVHPDPAKRYAELSEFIFDLRHPNRAFQSRTRAPLIERDPVAFWKGVSAVLAILVL
                     LLLRKLIASKG"
     gene            complement(10689..13457)
                     /locus_tag="VFQ61_17210"
     CDS             complement(10689..13457)
                     /locus_tag="VFQ61_17210"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET9956252.1"
                     /translation="MFKRDTLLEEQTRTDHEYGFVLACFREVLTELGEVELAESLSPE
                     GSKPGTAAISGLDERFVQASSICFQLFAIAEDLAVESVQRVLEAEPDAEVLPGSWDQN
                     LERLIALGLDERTIASALPSVRVEPVLTAHPTEAKRATVLDHHAELRTLLRARSQSVL
                     TPPKLEVLRDRVKTALERLWRTGEIFLDKPDLSSELRYIVHHLRDEMPEALLNADERL
                     RLAWVRYGFDPALLDDERNLPRVTWGNWVGGDRDGHPLVTSAVTRETLDELRRAALLI
                     LDERLVRLGAALSLSERLGRVPASLVTRRDALAEALGHRAGPALARNPGEPWRQVVNL
                     MRMRLPSDRAASGQGIVLSTESDRYTRAAELVSDLRIVHDALVEVGATRLARSDLLPV
                     MRIAQTFGFHLAALDVRQNSAFHERAVEQLLRAAGASDVDYSAWDEARRLAFLQDELA
                     RPRPFTREGCRLGSEAEATLGSYRVLAAHVQARGTDGVGALIVSMTRSLSDLLLVYLL
                     AREVGLTVYDESGGACVLPVVPLFETIADLQASPQILAAFLQHPFTRRSLRLHAAPGE
                     CPVQQVMVGYSDSNKDGGILASLWHVHRAEAALAAVGREHGVRVRFFHGRGGTISRGA
                     GPVHRFIRALPHGSLQGDLRITEQGEVIAQKYGDAQRGAHSLELLLANSTFATLLHKH
                     SEPAEHPLATILDELAQTSRHAYEALIQGEGFVQFFRQATPIDVLEGSRIGSRPSRRT
                     GKQSISDLRAIPWVFAWSQSRFYLSGWFGVGTALERLEREQPGAFRQIQRDFIEWSPL
                     HYIIGNVATSIAMADPALMREYASLVEDAELRERVLTHVLAEHDRTRRLLEQLYGGPL
                     EERRPRVGWGMARRAARLRALHLHQIELLREYRKLDRAAEPERTEGALLRLLLTVNAI
                     AGGLRATG"
     gene            complement(13653..>14303)
                     /locus_tag="VFQ61_17215"
     CDS             complement(13653..>14303)
                     /locus_tag="VFQ61_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956253.1"
                     /translation="ASGVVPRLEIYAVPDAAAFAGLESTLLKPKAAAVLATPPHEQLT
                     AVTGRVREAVERVWPTGSVGEPLTIQIGAHDPKVLEVRVVHLGPAVNSDGLEAMERSL
                     TTALDSPTRILDVPLPSEPLTRDQGDMQFVSRVVSALQSAASVPDVSVCVLRPEAAKR
                     RGQLSPDEPLATTLSSALKLHPRVVVATGNTWQIHFVHGACPALDQPADQRPRANP"
BASE COUNT         2606 a         4610 c         4458 g         2629 t
ORIGIN      
        1 ggcacctcgc cttgcgctcg cgagcgacca agcggtgatt agcccgcgcg atggcggcag
       61 cccatctcgt aaatctggac ggctcacggg ttcgtcggcc acgctttgcg cgggagaacg
      121 ccagcctcac cgtagcgacg caggcgagac agccagccag gccccggtga gccgccccga
      181 gcactaggac gcgcccattc gcgatagctc aacgggagct ccgtcccaaa gcgaaacgcg
      241 cggctctcgt atataaccca tgagtattat cgtatttgcc gcgctcgata ctcagtgaga
      301 ttctcactga gctctacaca cacgtcattt gcggatatgg ctcctgtcac tcgcgcggag
      361 ccatgttatt cttccgcgga tgtttgcgaa gcgcgtgtcg gctgcacaca gcagcgccag
      421 cgcgggccca atgggtctcg aaccggggac tcagctcggg aaccatcgag tcctgttctg
      481 caccgcgcgc ggcggcatgg ccagcgtctg gcccgccctg cagcttggtc cccgtgggtt
      541 cagcaagctg gtggcgttga aagccattct cccggacctg gtcgcgtcac cggagttcga
      601 ggcgctgttc atcgaagagg cgcggatcgc cgcgcgcatt cggcatccga acgtgtgcga
      661 agtcttcgag ctgatcgagg agaacggtgt gctcgcgcta tccatggagt ggatcgacgg
      721 cgacgcgctc ggccgggttt tggaaacgcc tccccaaccg ctgaatcctc ggattgccgc
      781 ctacatcgtg gcccaagccg cggctgggct ccacgccgcc cacgagctgc gtgacgacgc
      841 cggagatgcg atgaacgtcg ttcaccgcga tgtctcgagt cagaacatcc tcatctccaa
      901 ggatgggcac gtcaagatca gtgatttcgg tatcgccaag gccttcggga gcacacgaga
      961 cgcaacccaa gccggacggg tgcgcggcaa actcgcttac atgtccccag agcaggcctc
     1021 cgcagccgcc acggatcgaa gaagcgacgt gttctctctg ggcgtggtgt tgtaccaaat
     1081 taccatcgga cagatcccgt ttcggcaggc agacgatacc acgcgcaccc acctcacgcg
     1141 tctactcgag tgagcttacc gctcgccccg tgagctgtac cctgattatc cgatcgagct
     1201 cgaacaaatc gtgagtcggg ctttgtcccg tgacccggcc caacgctatc cgagcgccga
     1261 tgccatgcgc ctggcgttgg agggttggct cgcgcgcagc ggccccccag tcaacgaaag
     1321 agacttagcg cgcgtggtcc gcgagcgccg cgggggcgaa atcgccgcgc gtgagctcaa
     1381 gattcgcacg ctctccgaga ttgcttgttt gccgcggggc gcgcacacgg gaaaaaccct
     1441 ggaacccatc gcgctgggcc cagaaccagt gacgtggacg gaaaggctcg ctccgcgccc
     1501 acgcgcgagc cgaatttggc tgctcgcagg cggcgccgcc ttgttcacgc tctcgttggt
     1561 gattcgcgct cgaatcgtcg ggtcggaagc tgagtccgcc catgccggta ccaccagcat
     1621 cagatcggac gcggccccac ccgcccgaga tgtccggcca gccccacctc tggccctttc
     1681 agcggtacct ccgccagagc gcaacgtcac cccacccgca ggaacggaag ccgagaagcc
     1741 gagaagccga gaagccgaga agccgagcct gaaacgcgct ccgcaggccg tcaaggcttc
     1801 ggctgccacc cttcgggcgc cgatgccccc caaacgagag tccgcgcgcc ccactcctaa
     1861 tcctcgaacc aaacccacgt taggaccgct ggagcgcgag ctttgacgtg ggtgttgcat
     1921 atcgagtgga tttggcgcga atccctgaag gcaactctct ggcttacggc gctgggagtg
     1981 ggggtcaccg ggctggcatt ttcacctggt gtggccgtgg cccagtcgtc agatatcgag
     2041 tctgctcggc gttcgtttcg agagggcaat gacttactcg aaagaaacga gtatgcggca
     2101 gcactcgcta agttcgagcg cgcacacgag tcttgggcca acccgaaaat ccgtctgaac
     2161 atcgccacca cactgcgcgc actcgggcgc gcggcagacg cgggcaatgc gtacgcactc
     2221 tatctggagg aggtcggcgc ggactccggc aagcgggagg aagtcgagcg cgtgctcgac
     2281 gaactccgca gatcgctggc tagagtacag gtggcggaat atggggtctc gagagcggaa
     2341 ctcgacggtc gcaaaatctc gaattcgacc cgaggctact tcgtggaacc gggccgccac
     2401 tcactgattc tcacggggag ggcgggcacc tcgcggcgtg aggtggagct acgtgcgggc
     2461 gaactgtatc tgatcgagcc ggagccgaac cagccagaaa cacctgcggc aaccctgccg
     2521 gcgcgcgcgg agatgcccac gcggcaaccc gcgagcggcc tcagcgatgt gaatacccct
     2581 tcgaagctct tcgtgttgag tcgtgtcgac atcgagggct ccgggcgcgg cgcggttgcc
     2641 gcactggggt tcggttatga gctcgacccg cacgtcagag ccaacctggg cgctctaatc
     2701 ggtacgaacc aagggggctg gttggggatc cacgtcaatc catcggttgg gctgcttcga
     2761 ccgatgctgg gcgtctcggt gcccgccttc gtagtcgagg gcattcaccc cggcattgcc
     2821 ggagaactcg gcctgcagct cgccctggat tcacgctggt acccgttcgc gagggcgtcg
     2881 gtggtgcact ttccgggcct cgcaacgaac tacgtatcca ctctattcgt gccggcggca
     2941 ggcatggaag tgcgactctg aggatggctt ccctcgcctt gcgtggtgtc gcgctcggag
     3001 cgagctgggt agctccgctt gccgtaaacc tggcctgctc tgcgtcagac gcggtgccgc
     3061 cggagctcgc ccggcgcacg gctgctttgg aagcggttca gttcactccg atctcgaact
     3121 ccacattacc gaatgcgcgg tcgcatgcgg ggttcgtata cgaccccgcg atcgatggca
     3181 gcgtggcgtt tggcgggcat ctgccgagcg atgcaacgct ccagggactc agagacacct
     3241 gggtctggga cggagtgcgg tggaattcac cgccggctgg ttacccgcag cgcagcttcg
     3301 tgagtggcat atacgatcct tccagtggca agctcctcag ctatgggggc tacgcggcca
     3361 ccgcctacaa caacgacacg tgggagttcg acggcctgaa ctggagttct cgattcaacc
     3421 tactcacgcc gggcaagcgt tcctcctatg gcatggcgca cgactcctca cgtggagtaa
     3481 cggtgttatt cggcgggtac aatgggcagt ggcagggaga catctgggag tgggacggaa
     3541 cgctctggac cgaacgctgt acgaactccc cctgctcgac cagcccgaaa ccctcggcgc
     3601 gagccaacgc caccttcgta tacgacgagg cgcgcaaggt aacgctgcta ttcggtggtt
     3661 acggtccggg tggcagccgc tcggacacct ggctgtggaa tggctcgcag ttcgtgctcg
     3721 tgaagacggc aatcatgccg agtccacggg actcagccgc tgcgacttac gaccccgtca
     3781 cgcaacgagt tcttttgttc ggagggatcc agtcggacgg ggtcgagcaa aatgaccttt
     3841 gggcttggga cggcgttcaa tggcaatcca tcgcccagag tacgcccgca acccctcgtc
     3901 aaggagcggg gctggcttgg gactccaagc gtaggcgcgg cgtactcgtc gggggggaat
     3961 tcttgggcaa acccgtcgaa ccgtgggagt tcaccgtcag cggtaaccgc tgctcttcgg
     4021 atctcgattg tcatcaaagt agctgcctat ccggaatctg tgcctcggtg agtaccaact
     4081 cgggcagcgg tggttcggcg gaccccggtg ctggcggtga aagcgccaac ggcggggccg
     4141 caggtgacgg tggcgcgggc ggcgctacga acgagagccg aggaggcaac cccacgggcg
     4201 ccggcggctc tcttgccacg tcacctacga gcgagggggc gacgagcggc cgcgcgggca
     4261 gtggaggctc gggtggctca gggtcgagcg gcgcagggac acagggcggg catgcgggct
     4321 cgggcagtcc gatggtccaa gccggagctg attcaggtgg tgcaggccgc gatactggca
     4381 ccgagggcac acttgccgtg gtcccatcgg ctgcggagga cgccgtgaac ttttatgcgt
     4441 gttccgtgat cggcgttgct cacggttcgc ctttggattc tcgtgtctgc ctctcggcgc
     4501 tcgcggttgg cctgctgctc gtcgaacgca gacgccgcag acacaaaccc tagcacggct
     4561 ctcgcaccct attcagaact cccatatcgc cagcttcggc aagcgctggc gttcgtgcgg
     4621 cgactgcgag aacaacggtg gccctcgtca tctgggactc tcgaacgtac gtgtcgagga
     4681 cgtgaagatc caagctgtat gacggcgcga gtcagcggaa caccgctacc tgcatggtca
     4741 cgcaagcaga tatcgagtcc tggtagcggc tctaacgggg cggagggttg agggtcactc
     4801 aaccctccgg ccgcgcgtgc gcgatgcctc agtacacttt gcaatagctc ggaaaggacg
     4861 acggcaaccc cgaagtgtac ggctccggct ctacgccgaa cggatgcgcc ttcatgaact
     4921 cccagcccgc gctgtagagg gcactcgggg cgccgcaatg gccgccaccg tggtcgcagt
     4981 tgacgacgaa gctgccggcg ctcttcatgt gcgaatcgta agttgcgcta gtggtgctga
     5041 aggccaccac cacgacatca ctagagcctc cgtgcatcgt catcaccgcc ggcgtagttg
     5101 ccgggttctg aatgggttga cgagtcacct ctccgcccga gttcggaacc acggccgcga
     5161 tgtagttcga gcgaagggcg cccatacagc ccgcctgaag cccgcccgcg ctgcagcccg
     5221 tggtgtagat tctccgcgga tcgatgttgt ggtcgcgaac cgcgcaggcc gcgatcagat
     5281 ctgcaatctt gaagtcgtct ttgctgaacg tcgaggttcc cgaacaatcg cctccggtac
     5341 gtagcgagct ctggaacgaa acgatgatgc ccccttgatt caatatttcc tgacgcaccg
     5401 ccgccggcat catgatattg acttcgctcg cgctcgagcc agtaccatgc caatagaata
     5461 tgagggagcc ggtcccctcc ttcttggctc ccacctgcaa tgagatgccg ctcaaaccgc
     5521 cgatgctcgt ggtgccggtg gcgaagttcg ggcatgtgcc gctgattggt ggaagctgcg
     5581 gctgggaacc gctgctcgcg ccgccggtcg tgctcgcgcc gcccatcgcg atcgatccgc
     5641 cgccagcgct agttggagtg cccccggcgc ccgaacctcc catcgctccg gagccggact
     5701 gccccgttgc tccgccgaga gcgccgctgg cgccgcccgt gctcttcgca cctcccgccg
     5761 ctctcccacc gttaccgaac cagcctgcga ccccggagct acccccgatc gtggaaccgc
     5821 cgttggcaat gccgccttgt gccccgcgcc cgccgctccc agccgacgcc gaggttccgc
     5881 ccagagccgc gatgcttccg ccggagcccg taagactccc tccgttggtg ccgcctgagc
     5941 caacgttgcc tacaacgcca ccgctgctcc cagaacggcc tgcctgaacg gaagagccac
     6001 caaagccagg aacgccagca gcgtccccga atgggtcgct gccgctcgcg ctgcagcccg
     6061 catttgcaaa taggaccaat gtagttgcga cccagggcac gccaagccgc gaacggtcgc
     6121 gcacgaggcg ggcggtggat tcgaaggagt ggagcttttt gcctttcaca ccttccgagt
     6181 ggcggcgcac gcgcggcgcg ggtggataga tgcacaagtg atttggggca cacctcggag
     6241 gccagtacta aaggtcgctg ttacaccgtg ggtaggtgaa aaagagccgg ctcgcttgcg
     6301 ggtggataaa tctaccgtgg tgcggactcc atcctcgaga gtggtaaagc ttggcgtgct
     6361 cggcgtcggg atccgccagg gttcgtgcaa gcggcatcgg cgtcggctcg cgaaagcaag
     6421 ccggcagtca tggactcaac gcagtcgagg cgtctgccgg ggtgattccc cttgggcctc
     6481 gacttggtga acccgattcg gtccgacagg tccgcgtaca gtcccgctcg cccgattgga
     6541 cagttttcgg accccggcac ggagcctagg ctgcattcgt gaacggttca ggatttggcg
     6601 caccatgaaa gtcgtaatca tcggagcgag cgtcgcaggc tccgccaccg cgctcgcgct
     6661 cgcacgagcc gggcacgacg tgcagctttt cgagcggggc gagaccgcgg aaaccggggc
     6721 tggtttcgtg ctctggccca acgccacgtt cgtgctggat cggctccaga ttctcgatgc
     6781 aattggagag ctgagcgcgc gccccgcggc gatgacacgc ttcgatctcg ctggccaggc
     6841 tctcggaggt tgggacttgc tcgcactcga gcggaagatg ggttatccga gcctgagtat
     6901 tctgcgctcc gatctgctgc gcattttaca tcgagcactg cgtcgacagg gggtcgaggt
     6961 gcagactggg tgccgcgcca tcgagctgct cgattcacca ggtgtgcagt tcgagggcgg
     7021 gcgcacgatc acagccgatc tggtggtggg ggctgatggt cgaaggcact cggtcacgcg
     7081 ccggtatgtc cgcccggagc atgccgctat ttatcagggc ttcgtcaatt gggtgggagt
     7141 cagcccggtg cgcctttcga ggccgattgt ccatgactat gcggctgcgg agcgccgctt
     7201 cggaatcgtg cccgtcgcgg gagagaaggt ttattgggcg ggggccatgc gccgggactc
     7261 ggcccctgcc ccaaacgaaa cgctcagccg ggctgagctg cttgagctgt tcactggttg
     7321 gcctccggtc gttcgtgaag tgatcgagcg atccgagaag ggcgcgttca aaaccatcgg
     7381 gatctacgac gtcgagccga atttagcttg gcatcgcgga aacgtgctgc tgatcggaga
     7441 tgccgcgcac gcagcctgcc caacatcggg gcagggggtc agtcaggcgc tcgaagatgc
     7501 ttggcattta cgagactgcc tccctaagcg cgcaacgcgc gtttccgacg cgctcaccga
     7561 gttcacacga ctccgattcg agaagacgcg cgcgatcact caatccggac gtgcgctggc
     7621 tcgcgagcta ttccgtccgc agagcgcagg tgccgcagcg catcgcccat cgggcgccgc
     7681 gctcgatctg gcttcgctcg cctcattctg gggcgccgga ctcccgctgc agaaatcggg
     7741 gtcaactccc taatccgccg gtttgccgca tgcccggccg ctggttgata acactcgccc
     7801 ccgagcgttc ctgaaacaag cggctgctat cggcagaaat gtcgaagaaa catgctcgtt
     7861 acacagcacc cgcagcattc gacgcttcgc cacgacagcc gtccgcctcc aaagggtctg
     7921 tgcggccttg gcagacgttt gtctaaaacc agaaaggacc gcggctttct ctcatggcct
     7981 acctgcttcc gaccgaattc gtcaccaaga tggtcgacgc tggcgaatcc aagatcttca
     8041 tgtccacgcg cgatacggtg attcgcggaa caatggcggg tgcgctcttg gcactatccg
     8101 cggtgttcgc ggtgtcggtg acggtgcaga ccaattcacc tctgctcggc gcggttctgt
     8161 tcccggtcgg gttctgcatg ttgtacttgc tgggcttcga cttgctcacg ggcgtcttca
     8221 tgctgagccc tttggccctg atcgataagc gtcccggagt gacgatgggc ggcgttttgc
     8281 gcaactgggg gttggtgttt ctgggcaact ttgccggcgc gctcttggtc gcgttgatga
     8341 tgtcctacgt gttcacgtat gggttttcga cggatcccgg ccccgtagga gccaagatcg
     8401 cgggcatcgg aaaggcgagg accctaggct acgcgaccta cgggatggcg ggctggatta
     8461 cgatcttcgt gcgcggcatg ctctgcaatt ggatggtgtc caccggggtg gtcggagcga
     8521 tgatctcgac ccacgtgagc ggcaaagtga tcgcgatgtg gatgccaatc atgttgtttt
     8581 tctacatgac gttcgagcat tcggtcgtga acatgttcct cttcccgtcc gcgctgatca
     8641 tgggggggca gttctccgtc gctgactatt tcgtttggaa cgagattccg acggtgctcg
     8701 gcaacttggt aggtggcatc actttcacgg gactcacgct ctacagcacc cacgtgcgca
     8761 cggctcccaa gcgcaaagcc gccgtctccg cggcccccgc gggcgtggcc ggccgcgccc
     8821 aggcatgagg ctgagggcag tctgcgcgat gagcgccgcg ccggctgccc gcaaatcccc
     8881 atgcacacca cgctgcaagt cacactcgga caagcatcgg acaagggtcg aaagcccacg
     8941 aaccaggact ttcacgctgc ctcgatcccc agagagcccc tcgcgacttt gaagggcatc
     9001 tgtgtcgcca tcgccgacgg aatcagcacg agcgaggtaa gcgcggaggc gagcgaggcc
     9061 gccatccgaa gcttcatcga ggactactac tgcacctccg aagcttggtc ggtcaagaca
     9121 tccgtagagc gggtcatgag cgcggccaac tcctggttgc actcgcagac tctgcgcagc
     9181 ccctatcgtt acgagcgtga tcacggctac gtgtgcacgt tcagcgcggt cgtcttcaag
     9241 gcggccactg cgcacgtctt caatgtcggg gattcgcgag tctatcgcct cactgaaacc
     9301 gcgctcgaac agctgaccga agatcaccgc gttttgcgcg caccggggga gagctatctc
     9361 gcacgtgcgc tgggtgcggc cgcccatctc gagctcgact atcgcgcggt gccgctcgaa
     9421 ccgggcagca tcttcgtgct cgctaccgac ggtgtgttcg agcacatcgc tcccgatcgg
     9481 ctccgcgagt gcgtcgccga ccacgtcgac gatctcgacg aagccgcccg gcagctcatc
     9541 aaactcgcat acgaaaacgg cagcccggac aatctcacgg cgcagatcgt cagggtggac
     9601 gccgtacccg caacgacgtc ggcggagttg tttcacgagc tctcggattt gccgctcccg
     9661 cccctgctag aagagagaca acggttcgac ggctatacca ttgtccgcga acttcacgcg
     9721 agccaccgca gccacgtcta tttggcgctc gatgacgaga cgagcgagcg cgtcgtactc
     9781 aagattccgg ccatcgacct gcgcgaggac tctgccctac tcgagcgctt cctactcgaa
     9841 gagtggatcg cgcgtcgaat ccggaatccc catgtgctcg ccccgcgcac accacgaaag
     9901 cgcaacttcg tttacttggt gaccgaatac gtcgaaggcc agactctgag ccagtggatg
     9961 cgggatcatc ctcggcccaa catcgagcaa gtgagaagca tcgtcgagca ggtggcgcat
    10021 ggcttgaatg ccttccacaa gcacgagatg attcatcaag atctacggcc ggataacatc
    10081 ttgttggatc ccactggcac ggtgaagata atcgatttcg gctccgttca cgtggccggt
    10141 gtcgccgaag gcgtgacggc agaacgcacg gagattgcag gcactttgca gtacgcagcg
    10201 ccggagtatt ttctcggcga gccgggctcc aatcgttccg atttgttttc gctcggtgtc
    10261 attgcttatc aaatgctcgc gggacgcctg ccgtatggta ccgaggtcag caagtgccgc
    10321 acccgactgg cacgagcgca gctccgctac acatcggtgc tgagcgatga tcgcgagatc
    10381 cccgctttcg tggatgaggc cctgcgcaaa gcggtccacc ccgatcccgc taagcgctac
    10441 gccgaattgt cagaattcat attcgatttg cgccatccga atcgagcatt ccagtcgcgc
    10501 acgcgcgcgc cgctcatcga gcgcgatccg gtcgctttct ggaagggcgt gtcggccgtg
    10561 ctcgcgatcc tcgttctgct gctactccgc aagttaatcg cctcgaaggg ctgatggttc
    10621 gacatcttgc cgtgggttgc gagccgtcga gcctgcgcgc cgaacagcac gcaggctcga
    10681 acggtgcctc aaccagttgc gcgtagtccg ccagcaatgg cgttgaccgt caacaacagt
    10741 cgcagcaagg ccccttcggt acgctcgggt tccgcggcgc gatcgagctt gcggtactca
    10801 cgcagtagtt cgatttggtg cagatgcagc gcgcgaagcc gtgccgctcg acgcgccatg
    10861 ccccagccca cccgggggcg tctttcttcg aggggaccgc cgtaaagttg ctcgagcagc
    10921 cggcgggtac gatcatgctc cgccaagacg tgggtcaaga ctcgctcgcg cagctcggcg
    10981 tcttcgacca aggaagcgta ctcccgcatc agcgctggat ccgccatcgc gatgctggtc
    11041 gcgacgttgc caatgatgta gtgcagcggc gaccactcga tgaaatctcg ttgaatctgg
    11101 cggaaagctc ccggctgctc acgttcgagg cgctcgagcg ccgtgcccac gccaaaccaa
    11161 cccgaaaggt agaagcgcga ttggctccat gcgaagaccc aagggatagc acgcaagtcg
    11221 ctgatcgact gtttaccggt gcgtcggctg ggtcgagatc cgattcggct gccttcgagt
    11281 acgtcgatcg gcgtcgcctg tcgaaagaac tgcacgaatc cctcgccttg gatcagagct
    11341 tcgtatgcgt gccggctcgt ctgagccagc tcgtcgagga tggtggcgag cgggtgctcg
    11401 gccggttcgg agtgcttgtg gagcaacgtc gcgaaggtgc tattcgccag caacagttcg
    11461 aggctgtgtg cgccacgttg ggcatcacca tacttctgcg cgatgacctc gccttgctcg
    11521 gtgatacgta ggtccccttg caaagagcca tgcggcaggg cgcgaatgaa gcgatgcacg
    11581 ggacctgcgc ctcggctgat cgtgccacca cgaccgtgga agaagcgcac tcgcacgccg
    11641 tgctcgcggc caaccgcggc gagcgccgct tcggcgcggt gcacgtgcca aaggctggcg
    11701 aggatgccgc catccttgtt gctgtcgctg tacccgacca tcacctgctg gaccgggcat
    11761 tcacccggcg ccgcatgaag ccgtaagctg cgtcgagtga acggatgctg cagaaaggcc
    11821 gccaggattt gcgggctcgc ttggagatcg gcgatggtct cgaatagcgg gacgaccggc
    11881 aacacgcaag ctccgccgct ctcgtcgtaa acggtcaacc ccacctctcg cgcgagcaga
    11941 taaacgagca atagatcgga gaggcttcgc gtcatgctca cgatcaacgc gccaactccg
    12001 tccgttccac gtgcttgcac gtgggctgct aacacgcggt aggaacccag cgtcgcctcc
    12061 gcttcactcc ccaaacgaca accctcgcgc gtgaagggcc gcggcctggc taactcatcc
    12121 tgcaagaagg ccaaacggcg ggcctcgtcc caagccgaat agtcgacgtc gctcgctccc
    12181 gccgcgcgca gcagttgttc tacggcccgt tcgtggaacg cgctgttctg acgtacgtcc
    12241 agagccgcga ggtgaaaccc gaacgtctgc gcgatgcgca tgacgggcaa caagtcacta
    12301 cgcgccagcc gcgtcgcccc gacctcgacc aacgcgtcgt ggacgatccg gagatcggac
    12361 accaactccg cggctcgagt gtagcgatcc gactcggtgc tcagcacgat gccctgcccc
    12421 gacgcggcgc gatccgacgg caatcgcatt cgcatcaagt tgacgacctg ccgccaaggt
    12481 tcacctgggt ttcgcgcgag agccggcccc gcgcggtgac ccaaagcctc cgccagcgca
    12541 tcacgacggg tcaccaacga tgctggaact cgccccaagc gctcggacaa gctaagcgcg
    12601 gcccccagcc gtacgaggcg ctcgtcgaga atcaacaatg cggcgcggcg cagctcgtcc
    12661 aaagtttctc gtgtaaccgc ggaggtcacg agtggatgtc cgtctcgatc gcccccaacc
    12721 cagtttcccc aagttactcg gggcaaattg cgttcgtcgt ccaacagcgc cggatcgaag
    12781 ccgtaccgca cccacgctaa ccgaagccgt tcgtccgcat tcagcagcgc ctccggcatc
    12841 tcgtcccgca agtggtgaac gatatagcgg agttccgagc tcaagtccgg cttatccagg
    12901 aagatttcgc cggttcgcca gaggcgttcc agcgcagttt tgacccgatc gcgaagcacc
    12961 tcgagcttgg gcggcgtcag cacggactga ctgcgcgccc gcagcagcgt gcgcaattct
    13021 gcgtgatgat cgagcaccgt cgctcgcttt gcctcggtgg ggtgagccgt cagtacgggc
    13081 tccacccgca cgctcggcag cgccgatgcg atcgtgcgct cgtccaaccc gagcgcgatc
    13141 agtcgctcca gattttgatc ccagctgccg ggcagcacct cggcgtcggg ctccgcctcg
    13201 agcacccgct gaacgctctc gaccgcgaga tcttccgcga tcgcgaatag ctgaaagcaa
    13261 atcgagctcg cctgcacgaa gcgctcgtcc aagcccgaga ttgccgccgt tcccggtttc
    13321 gagccctccg ggctcaagct ctccgcgagc tccacctcgc caagctcggt cagaacctct
    13381 cgaaagcaag ccaggacgaa accgtattca tggtcggtcc tggtttgctc ttccagcagc
    13441 gtgtcgcgct tgaacatggg ccgtgtatag cgccagctca gcaccgttca aaccaccaac
    13501 ccttggaaat gaacccaccc aaagaggacc gacagcctac gagaagcacg acgatgtcag
    13561 tcgcgtgtca ccgccggaag tcgcttccgt tcgagtttgg gaggcttccc tgtctgtttc
    13621 cgtagctcga tcgtttcatg attcgcgcgg tttcaaggat ttgcgcgcgg ccgctgatca
    13681 gctggctgat caagagccgg acaagctccg tgcacgaagt ggatctgcca agtgtttccg
    13741 gtcgcaacga cgaccctcgg atgaagcttc agggcgctgc tcaatgtggt cgccagaggc
    13801 tcgtctgggc tgagctgccc ccgccgcttc gcagcttccg gccgcaaaac acagacgctc
    13861 acgtcgggca cgctggcagc cgattgtagc gccgacacca cccgagagac aaactgcata
    13921 tcaccctgat cacgcgtcag tggttcactc gggaggggca cgtcgagaat acgtgtcggg
    13981 ctatccaagg ccgtggtcaa gctgcgttcc atggcttcca gcccgtccga gttgactgcg
    14041 ggcccaaggt gcacgacgcg cacttccagc actttgggat cgtgagcacc gatctggatt
    14101 gtcagaggct cacccacgct gccggtcggc caaactcgct cgacggcctc gcgcacccta
    14161 cccgtcaccg ccgtgagctg ttcatgcggc ggtgtcgcca gcaccgcggc cgcttttggt
    14221 ttgagcaggg tggactcaag gccggcaaat gcggccgcat ccgggacggc gtaaatctcg
    14281 agccgcggaa ccacgccgga cgc
//
LOCUS       DASTKU010000499        16608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_95709,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000499 DASTKU010000000
VERSION     DASTKU010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16608
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95709"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..999)
                     /locus_tag="VFQ61_17220"
     CDS             complement(148..999)
                     /locus_tag="VFQ61_17220"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HET9956254.1"
                     /translation="MSNTVDVDAYFARIGYDGPREPTLETLSRLHGLHPQAIAFENLS
                     PLLGESVSLDLGTLQEKLVQGARGGYCFEQNSLFKALLQALGFQVVGLAARVVWNQPE
                     DAVTPRTHMLLKVQLPEGPYLADVGFGGQVLTGPLRFDSTVAQATPHEPFRLGTTGNG
                     MQLQSLVRGEWKTLYRFGLDEQFPIDYELSNYYVSTHPNSIFRQRLMVARVLGNLRHN
                     LVNDQLTTHQVDARTGSSTQSEHRHLESVAELRAVLTDVFGLNVPRGSDADLALGRVL
                     TAPARMS"
     gene            complement(1208..1807)
                     /locus_tag="VFQ61_17225"
     CDS             complement(1208..1807)
                     /locus_tag="VFQ61_17225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9956255.1"
                     /translation="MRNLALFDFDGTITSRDTFTPFILHAVEPLRMAIGKVVLSSLIV
                     AYKLGFLSASRMRRSVVHVGLRGRRYEDVRVLGRDYARRALSLVVRPKALERIRWHQS
                     HGDLVVVVSASLDVYLTDWCREVGVELICTELEQRLGVLTGRYRDGDCSGPEKARRVR
                     QRYDLGSYGTIYAYGDTVEDEAMLALANQPFYRWRELHD"
     gene            complement(1927..4068)
                     /locus_tag="VFQ61_17230"
     CDS             complement(1927..4068)
                     /locus_tag="VFQ61_17230"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9956256.1"
                     /translation="MSYEPWMGNLVVGSTLGNYGTVKFLLDEEHADSALSVRVRVEIA
                     AGEFSPGDFEVEVFTNLNRRDFVKAYEPLAEALNPASSYWLSTPMQYVGKSYANFVYE
                     VTLPIQKCGAYRLTTRYRRTDSSEFYWHNQFSPGFGAAQHRDCAIVVSPTKAARLRIY
                     EANALTVEATRGGDYDNRSTFDDFLPSHDFDGFNPFQLDYVTRTLGFNTLWLMPIFPN
                     TRWRWDVPRWQWAENDSPGSPYAARDYYSVNPWLADTGGAQRAFDLFKEIIDRGHEGG
                     LDVFIDVAFNHAGRDVVYGAGGVELGICAPSEADSWIREVRPSFCTRGSEFRWDGIVP
                     HYRERADSGFACAVWAPTDRLNEHVWDDGNVDWYFGDYSTLGPKPGVYYDPRGNAEDE
                     RDLYYSDLGADQETAALWRYFAHILPFWLGRTDNKLAGIRADFAQGLPNQLWEFIINT
                     TRKSRWDFVFLAEVLDPDVIQYRLNRVFDVLSSKDHYLYRSSDVRMSQLFGSLEAEAN
                     VLGGGALLMHNGTSHDEQGNPDKWAMVARYAVAASLYGTPMVFMGQPLGLADKLPFRN
                     SFANMYDAWTSEDPERAPVAEMYRRINTVRESSIELREKNRYFLQQLQGGFRENIFSL
                     ARWVPAGDVDSVVLVFVNLSTTQPGGAVFAIPRSVRLTGNYQARNLAAIDPDRDLWPV
                     ARSAEDIYRFGVYVAFSYPNEVQILRLVRLG"
     gene            complement(4193..4801)
                     /locus_tag="VFQ61_17235"
     CDS             complement(4193..4801)
                     /locus_tag="VFQ61_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956257.1"
                     /translation="MHRGVQIWAVLGALTWAGLARAQGADARAELASEMLAANRSEET
                     PPVAASSREAKHWYGWQTLLVDGAALGIVAGGFAVRPAHSDWSVVSVVGVGAWVLGAP
                     AVHLAHGRVGVSFASLGLRLALPALGGYLASRPCSSGECSAQTLGVLVGAALIPAPML
                     IDAVWLAREPREGSRSARRTVNLFPFATSSERRRFLGVAGTF"
     gene            4993..5562
                     /locus_tag="VFQ61_17240"
     CDS             4993..5562
                     /locus_tag="VFQ61_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956258.1"
                     /translation="MVVVVTRILARSVLKFRLAGAILPVFTAGAGCKEVTEAIVGKGV
                     QAAKDATKGVSDGIEKGRKEGQSSDGALIVSKPEEVTGKGSIAVRALRARAGEPRRAE
                     VELAFENLSERPMRFTGLNPIALDRENFVQRPLEPTHEVTVPPKAKERLTFLFEAEPS
                     SLAKVRVWGVEYPLPAPTPPPPASASAHP"
     gene            complement(5607..6194)
                     /locus_tag="VFQ61_17245"
     CDS             complement(5607..6194)
                     /locus_tag="VFQ61_17245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9956259.1"
                     /translation="MSTSDMRRAHRELEERLRPFVSRRVPSCDVEDVLQEVFVRMQAA
                     LPALRNEDLLVPWLYRIAKSAIGDSHRRRLRAPRLEHDSEVLEEGSLGLDRESNEPSD
                     LEQELSSYMVTFIAKVPSPYREALTLTEIEGLTQRAAADALGVSLSAMKTRVQRGREK
                     LREELESCCRIATDARGRVIDCVPRDPSDCRCRGS"
     gene            complement(6194..7027)
                     /locus_tag="VFQ61_17250"
     CDS             complement(6194..7027)
                     /locus_tag="VFQ61_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HET9956260.1"
                     /translation="MSHETADEIRSSVREHYGKVADTAGAVGCAPSCCAGKPGSSAAL
                     GYSKEEMAAVPEGADMGLGCGNPQALAGLKPGETVLDLGSGGGFDSFLAARQVGPSGS
                     VIGVDMTPQMVTKARDNARKLGARNVEFRLGEIERLPVADSTVDVILSNCVINLSPSK
                     PDVFREAFRVLRAGGRLAISDVVALAPIPTEFRSTAASLSACVGGAALVTELEAMLLD
                     AGFTAIRITPRAESGEFIKDWMPGSGVERFIASANIEAIKPGAEQKCCGPECCTEKAR
                     S"
     gene            7176..7535
                     /locus_tag="VFQ61_17255"
     CDS             7176..7535
                     /locus_tag="VFQ61_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9956261.1"
                     /translation="MNQSQAAQCCPGAEQPGGEQDALELRPVEGDDADEELANLAKAL
                     SHPARVKIVRLLERKNSCICGDLVEELPLAQSTVSQHLKVLKAAGLVRGEVDGPRVCY
                     CIEPRTLRRLKALVGGL"
     gene            7580..7969
                     /gene="arsD"
                     /locus_tag="VFQ61_17260"
     CDS             7580..7969
                     /gene="arsD"
                     /locus_tag="VFQ61_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HET9956262.1"
                     /translation="MSITIRVFDPAMCCSTGVCGPSVDASLARFAADLEWLRGQGVTV
                     ERFNLSQQPAAFAETAVVREALQRGTEVLPLILVGDRIAVEGAYPARETLAALAGVVV
                     KKLDVVAGAATSCCGPSADSKSGSGCC"
     gene            7980..9758
                     /gene="arsA"
                     /locus_tag="VFQ61_17265"
     CDS             7980..9758
                     /gene="arsA"
                     /locus_tag="VFQ61_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical pump-driving ATPase"
                     /protein_id="HET9956263.1"
                     /translation="MNLIDAAPRNLFFTGKGGVGKTSVACATAVRLAEQGRRVLLVST
                     DPASNLDEVLETELGGEPRVVTGVPNLFALNLDPEAAAHAYRERVVGPYRGLLPEAAV
                     RSIEEQLSGACTVEIAAFDEFSKLLGDASATASFDHVVFDTAPTGHTLRLLELPAAWA
                     GFLEGNAGGTSCLGPLAGLQAQQSLYRATRAALVDAERTLLVLVARADRAALKEAERT
                     RTELAALGITRQVLILNALFVASDARDLVAVGMQRQGERALGEMAPALAALERVELRQ
                     LPFGLVGVAALRTLFAGVDAGSESASYPPRQSSESSARGLGALLPELEAAGRGVILTM
                     GKGGVGKTSVAVEIAVHLAQRGHSVHLTTTDPAAHVADALGSPVPGLQVSRIDPEAET
                     RSYCDEVMRTAGANLDAAGRALLEEDLRSPCTEEVAVFRAFARTVEHGEHGFVVIDTA
                     PTGHTLLLLDAAEAYHREVLRSAGSAPEEVRRLLPRLRDPEFTKVLLVTIPEATPVHE
                     ARALQRDLERAGIHPFAWVVNQSLVPLRVTDPTLVQRRENERRFLEEVRELSVRNAII
                     PWLTPRLAEQRSELALDTARVAGGMS"
     gene            9761..10870
                     /gene="arsB"
                     /locus_tag="VFQ61_17270"
     CDS             9761..10870
                     /gene="arsB"
                     /locus_tag="VFQ61_17270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HET9956264.1"
                     /translation="MAASNSMVMPEASVGVARKLSFLDRYLTVWIFAAMALGVGLGYV
                     APGFTAALDRMSVGTTSVPIAIGLILMMYPPLAKVRYEELGRVFRNTRVLSLSLIQNW
                     IVGPLLMFGLAVLFLRDKPEYMLGLILIGLARCIAMVIVWNDLAQGDTEYCAGLVALN
                     SLFQVLFFSLYAYLFATLLPSWLGLEGAVVHITIGEIAESVAIYLGIPFAAGFVSRRL
                     LVARKGRDWYETRFVPRIAPITLIFLLFTIVVMFSLKGETIVQLPLDALRIALPLLVY
                     FLLMFAISLVMSKRVGATYAQSATLSFTAASNNFELAIAVAIASFGIHSGAAFAAVIG
                     PLVEVPVLIGLVNVALWAQRRFFAPAPRSGLGLNS"
     gene            10888..11319
                     /locus_tag="VFQ61_17275"
     CDS             10888..11319
                     /locus_tag="VFQ61_17275"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HET9956265.1"
                     /translation="MTSNSKSILFLCVANSARSQMAEGLARALFGERIPVESAGSEPS
                     KVNPYAIEVMQERGIDLRAHHSKSVQTVDPQRVGIVITLCAEEVCPVFLGRAERLHWP
                     IPDPASQDRSISREEMLSRFRTARDTIQSKLEQFQKERGLG"
     gene            11316..11765
                     /gene="arsN2"
                     /locus_tag="VFQ61_17280"
     CDS             11316..11765
                     /gene="arsN2"
                     /locus_tag="VFQ61_17280"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HET9956266.1"
                     /translation="MKQIIVPAAESDLGAVLLLLQQVSLPVAGVADEFPGDYCVARLN
                     DEVVGVAGLESYGEFGLLRSVAVHSSRRGSGLGRLLVEDRLVAARARELTSVYLLTTT
                     AAAYFAVFGFKIASRGAAPENIRASLEFQSICPASAVCLVRDLRANE"
     gene            complement(11809..12729)
                     /locus_tag="VFQ61_17285"
     CDS             complement(11809..12729)
                     /locus_tag="VFQ61_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956267.1"
                     /translation="MMSISSKSRVLRWGVLAFLLSSCAKDAQRRESSAPSAAVGAPGT
                     AAPQRASAAVTLKCPPEAPSAGPIPIQVTLNADARTYWDNQGVLDNALEVLLIRRDQP
                     GLISIAKVDPDAMMLPQSPPRGRPSDAELASDRARIIEQKQYDLLAYGRAHPGAGDYF
                     VVATFADAWTGPTELRIKDAAGPMAPDPASAQLALAEAGDAPIPAAPSATGTVRVSER
                     AAVVGAFREIATSSEPVLTIVLARLRSSGGVLTRQYRLTLQHDKRDLIGTFSVPLASL
                     SAPNPAPSGRHVLFVFVGNQSATPNVIEVP"
     gene            13066..14565
                     /locus_tag="VFQ61_17290"
     CDS             13066..14565
                     /locus_tag="VFQ61_17290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9956268.1"
                     /translation="MQRPPAPNSDPPSSFLPGTVIDGKFEVRRLLGVGAMGAVVEATH
                     LLRRARVALKFMSEAVAHSPGVVERFLNEGIAASRINNEHVVRVLDVSRLPNGQPYLV
                     MEFLEGEDLQSLLQREGRQGLPDVARAVHLVLQVLRGLQVAHHAGIIHRDLKPANCFV
                     VTEDGEPDFVKIVDFGISKVQQGDGTLKLTTEGASMGTPLYVSPEQARDAMNVDARSD
                     IYSVSAMLYELLCGRTPFEATTLMGLFMQLATTMPEPVGARRPNLPAGLSEVVQRGLA
                     KQPSERFQSAAEMAQALAPYADARSEGLLLKLAQRPSLSASRPSSRPPSRAGGSAVSV
                     GDRTQRSKGIGSQPPHSPSSNALTMDAPAAAPSRARVRLVLGSLAVVLLAAAAAAGLR
                     WFKSSAETTPAASATSSPSSSAAPESAPRSGQPTVIPVPSVEVSAQVQASGSSSATMP
                     GGTGPSAPPPIRAKSDPSVSASGKNGSTGAETVRPKGPRPSLGKIGVQD"
     gene            14603..15826
                     /locus_tag="VFQ61_17295"
     CDS             14603..15826
                     /locus_tag="VFQ61_17295"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9956269.1"
                     /translation="MFSPSKHSSLVLLSAWSLCFSSAIPMAQAAPPAQAAPKTQSQPK
                     APAAPKVQAKAPAAPTVREELSADAQRDWDAARELYDARDYRGALVHFEKAYELSRNP
                     RVLFNVGVCWKDLTRYAQAIRVWERQLEFGARLSLEDLERTKSAIAAARPFVSTLTLQ
                     SDQPGAVILIDGVEAGTTPVTEGLPVDVGRHTVTLKKDGFAPSEAAVDVIQGTPAALT
                     LNMTPLVKLGTVAITVVGPRSATVFVDGRELGPAPFTGTVQEGPHTFEARAPGYLPAR
                     QTTEIRYAERSSLTLALAEARNEGKVRIVTDYPDAAIAVDGEVKGSGAWEGLLPAGGH
                     QLRVQKSGFKTHVTELSLSAGQQRSLQVHLEKQQSWLWWTVGIATVVGGGTVAAILLS
                     RPTDTSPVTGTLGTF"
     gene            15858..>16608
                     /locus_tag="VFQ61_17300"
     CDS             15858..>16608
                     /locus_tag="VFQ61_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956270.1"
                     /translation="MGYQTRFSHERLLRGLGFTASLGLTALGSLGLGACSGDRPTEIV
                     AGMMTQLRVPDDIRAVGVVVQQGGRVEFCESYSVTDGTASLPATIGWLNEGRSGKGPV
                     TVQVLGLRTEDSAFTTDCVTTRPESLGKQVRVIRRRRLSFVDDHITYLPLPLKDSCRD
                     ISDCNEDETCVGGKCVAMDIDATTLPDYKDSFVFGNTNTCFDGNLCMPKGKTLPVSLT
                     DLNDCSFEVPVPEQAQIPGGELNVRMFYESFG"
BASE COUNT         2971 a         5175 c         5308 g         3154 t
ORIGIN      
        1 cgatagtatt cctcaacggg ccgccaacgg accggcaccc gctccagcga gctcgaatcg
       61 tattcgacta ctagatcgct cacctctagt aagtcttccg gttcgagtga cgattgcatc
      121 acgcaagcct aaatcgatat cgcaggacta gctcattcgc gcgggggcgg taaggacccg
      181 cccgagcgcg aggtcggcgt cagaacctcg gggcacgttc aacccgaaca cgtccgtcag
      241 caccgcgcgt agctccgcca cgctctccag gtggcggtgc tcgctctgcg tactcgagcc
      301 cgtccgcgcg tccacctgat gcgtcgtgag ttgatcgttc acgaggttgt ggcgcagatt
      361 gcccagaact cgcgccacca tcaagcgttg gcgaaagatc gagttgggat gagtcgagac
      421 ataataattg ctgagctcgt aatcgatcgg gaattgctca tccaacccga atcggtacag
      481 cgtcttccat tcgcctcgga ccagcgactg cagctgcata ccgttgcccg ttgtccccag
      541 tcggaaaggc tcgtggggtg tggcctgtgc caccgtgctg tcgaatcgca gcggcccagt
      601 caatacttgc ccgccgaagc cgacgtccgc caagtacggc ccttcgggca gctgcacctt
      661 gagcaacatg tgagtgcgag gcgtcaccgc atcttccggt tggttccaga ctacgcgcgc
      721 ggcgaggcct actacctgaa aacccagagc ttgcagcagc gccttgaata gcgagttctg
      781 ttcgaagcaa taaccgcctc gagccccttg caccagcttc tcctggagcg tgccgaggtc
      841 gagtgacact gattcaccca gcaacggact caaattctcg aaagcaatcg cctgcgggtg
      901 caagccgtgc aaccgcgata gtgtctcgag cgtgggttca cgcggcccgt cgtagccaat
      961 gcgagcaaag taggcgtcca cgtccacggt gttcgacatg ggcgaaagct agttgcgctc
     1021 accagaaaag tccaacgcgc ggaaatgcgg gcagtgtggc tcgtcttggc gcggagttta
     1081 ttgcgggcgc cagcgcggtg tggtgtgggt tgttgggaaa ttggggctgc gcgttcgagc
     1141 gccggggacg aggagtcgcg ctcgttttct gggtggcgaa cagaccttca gtccggaagc
     1201 gcgggcctca atcgtgcagt tctcgccatc gatagaaagg ctgattcgcg agcgcgagca
     1261 tggcttcgtc ttcgacggtg tcgccgtagg cgtaaatggt gccgtaactc ccgaggtcgt
     1321 agcgttggcg tacgcgccgg gccttctcgg ggccgctgca gtcgccgtct cggtagcggc
     1381 cagtcaagac tccgaggcgt tgctcgagct ccgtgcaaat cagctcgact ccgacttcgc
     1441 ggcaccaatc ggtcaagtag acgtcgagcg aggccgagac gaccacgacg aggtctccgt
     1501 gcgactggtg ccagcgaatt cgctctagcg ccttcgggcg aacgactaac gacagagcgc
     1561 ggcgtgcgta gtcgcgtccc aagacccgta catcctcgta acgccggccg cgcaatccga
     1621 cgtgcacgac acttcggcgc atgcggctgg cggataagaa tccgagtttg tacgcgacga
     1681 tgagcgagct gagcacgacc tttccgatcg ccatgcggag cggctccacc gcatgaagaa
     1741 tgaacggggt gaaggtgtcc cgagacgtaa tggtgccgtc gaaatcgaat agagccaggt
     1801 tccgcaagtg tttaggctta ttactcgaac ccgccggctg tcgcgagcag ctgccaggcg
     1861 aattggcggg cgtccttgga gagggccctc agaatttgaa ccaaagttca ggctcgcctg
     1921 ggcgggttag cccaggcgca ccaatcgcaa aatctgcact tcgtttggat aactgaaggc
     1981 cacgtagact ccgaagcgat aaatatcttc agcgctgcgc gcgacaggcc acaggtcacg
     2041 gtctggatcg atggccgcga ggttgcgcgc ctgatagttg ccggtcaacc gaacgcttcg
     2101 cgggatcgca aacactgcgc cccccggctg agtggtgctc aggttgacga agacgaggac
     2161 caccgagtcg acgtcgccgg ccggaaccca acgtgcgagg gagaagatgt tctcgcggaa
     2221 gccaccctgg agctgctgca ggaaatagcg attcttctcg cgcagctcga tcgagctctc
     2281 gcggaccgtg ttgatgcgtc ggtacatttc ggcgaccggt gctcgctcgg gatcctcgct
     2341 cgtccaagcg tcgtacatgt tggcgaagct gttccgaaaa ggcagcttgt ccgcgagccc
     2401 gagcggttgc cccatgaaca ccatgggcgt gccatacagc gaggccgcga cggcgtagcg
     2461 cgccaccatg gcccacttgt cggggttgcc ttgttcgtcg tggctggtgc cattgtgcat
     2521 caacagcgca cctccgccaa gcacattggc ttccgcttcc agcgagccga agagctgact
     2581 catgcggaca tcgctcgaac gatataggta gtggtctttg ctgctcagca cgtcgaaaac
     2641 gcgattgagt cgatattgga tcacgtccgg atccagcacc tcggcgagga acacgaagtc
     2701 ccagcgtgac ttgcgtgtgg tgttgatgat gaactcccaa agttgattgg gcagcccttg
     2761 cgcgaagtcc gcgcgaatgc cggccagctt gttgtccgtg cggccgagcc aaaacggcag
     2821 gatgtgcgcg aagtacctcc aaagcgcggc ggtctcctgg tctgcgccca aatcactgta
     2881 gtacaggtcc cgctcgtctt ccgcgttccc gcgaggatcg tagtagactc cgggcttggg
     2941 acccaacgtc gaatagtctc cgaagtacca atcgacattt ccgtcgtccc agacatgctc
     3001 gttcaggcga tcggttggcg cccagaccgc gcacgcaaac ccgctatccg cccgctcgcg
     3061 gtaatgcgga acgatcccgt cccaacgaaa ctcactgcct cgcgtacaga aggaaggtcg
     3121 cacttcgcga atccaggagt ccgcttcgct cggcgcgcag atgccgagtt cgaccccgcc
     3181 cgcgccgtaa acgacatctc ggcctgcgtg attgaacgcc acatcgatga agacgtcgag
     3241 cccgccctcg tgtccccgat cgatgatctc tttgaacaga tcgaaggccc gctgggctcc
     3301 accggtgtct gcgagccagg ggttcacgct gtagtagtcg cgcgcggcgt aggggctgcc
     3361 ggggctgtcg ttctcggccc attgccagcg cggcacgtcc caacgccagc gcgtattcgg
     3421 gaagatcggc atcagccaga gtgtgttgaa gcctagtgtg cgcgttacat aatcgagctg
     3481 aaacgggttg aagccgtcga agtcgtggct cgggaggaaa tcatcgaagg tgctgcgatt
     3541 gtcgtagtca ccacctcgcg tggcttccac cgtcagcgcg ttcgcctcgt aaatacgtag
     3601 ccgagcggcc ttggtgggcg agacgacgat cgcgcagtcc cggtgctgag ctgccccgaa
     3661 ccctggcgag aactgattgt gccaatagaa ctcgctcgag tcggttctgc gataacgtgt
     3721 ggtgagccgg tatgctccgc acttttggat tggcagagtt acctcgtaca cgaaattcgc
     3781 atacgacttg ccgacgtact gcatcggtgt cgacagccaa tacgatgacg ctgggtttag
     3841 tgcctccgcg agcggttcat aggcctttac gaaatcgcgg cggttcaagt tggtgaagac
     3901 ttcgacttcg aaatcaccag gggaaaactc accggcggca atctccaccc gcacgcgcac
     3961 cgaaagcgcc gaatcggcat gctcctcgtc gagcaggaac ttgaccgtcc cgtagttgcc
     4021 cagggtgctg cccaccacga gattgcccat ccacggttcg taactcattc cgttcctcct
     4081 cgagcgctcc gcgatcggtt catcgccgca gagggagtgt cataccgttt ggctccgcgt
     4141 caatcaggac ggaacaaaca accatgtacg gcgatatccg gacactcgcc cttcagaagg
     4201 tccccgcgac gcccaagaaa cgtcgccgtt ccgagctggt cgcgaacgga aacaagttca
     4261 ctgtgcgtct cgccgagcgc gaaccctcgc gcggttcgcg agcgagccac accgcatcga
     4321 tcaacatcgg cgccggaatc aaggcggcac cgaccagaac tccgagcgtt tgagcgctgc
     4381 attcgccgct cgagcaaggg cggctagcca gatagccccc gagtgctggc agcgccaatc
     4441 gcagccccaa gcttgcgaaa ctcactccga cgcggccgtg tgccaggtga acggccggcg
     4501 cgccgagcac ccacgcgccc acgcccacca cggaaaccac gctccagtcg gaatgcgcag
     4561 ggcgaaccgc aaacccgcct gcaactattc cgagcgcggc cccatcgacc agcaatgttt
     4621 gccaaccata ccagtgcttt gcttcacgcg atgaggctgc gacgggaggc gtttcttcac
     4681 ttcgattcgc cgccagcatt tcgctcgcga gctcggctcg cgcatcagcg ccctgggcgc
     4741 gagccaaccc cgcccaagtc agcgcgccca aaacagccca gatctggacc ccgcggtgca
     4801 tcggcctaga agctagcgca ccctggcgcc attctctcgc tctatttgtc gaggacggac
     4861 cgcgccttgg tccgagacca atcgagctag cgagttggcg tccggaccgc gccgttctcg
     4921 atgacggcgc cgatccatgg cacccctctg gtctatggca cccgtctggc ccgagctggt
     4981 atggttcgcc gcatggttgt ggttgtgact cgaatccttg cccgctcggt gctcaagttt
     5041 cgcctggccg gcgccatcct gcccgtgttc accgcgggcg cgggttgcaa ggaggtcacc
     5101 gaggcgatcg tgggtaaggg cgtgcaagcc gccaaagacg caaccaaagg cgtctccgac
     5161 gggatcgaga agggtcgtaa ggaaggccaa tccagtgacg gcgcgctgat cgtttccaag
     5221 cccgaagagg tcacgggcaa aggttcgatc gcggtgcgcg cgctgcgtgc tcgcgcgggg
     5281 gagccgcggc gagccgaagt cgaactggcc ttcgaaaacc tcagcgagcg gcccatgcgc
     5341 ttcactggcc tcaatcctat cgctttggat cgcgagaact tcgtgcagcg cccgctcgag
     5401 cccacgcacg aagtcaccgt gccccccaag gccaaagagc gcttgacctt cctgttcgaa
     5461 gccgagccaa gcagcctcgc aaaagtccgt gtctggggcg tagaatatcc actcccggcc
     5521 ccgaccccgc cgccccccgc gagcgcctcc gcgcacccgt gaggatgccg tgcttgctcg
     5581 ctacggggtc tcgcgtttat cgctcgtcac gagcctcggc agcggcaatc ggatggatcg
     5641 cgcggtacac agtcgatgac tcgtcctcgc gcatccgtcg caatgcggca gcaagactct
     5701 agttcctcgc gcagcttctc gcggccacgc tgcactcgag tcttcatcgc agatagagag
     5761 acgcccagtg cgtccgcggc ggctctctgc gtgagccctt cgatttcggt caatgtcagc
     5821 gcttcccgat acggtgatgg gactttggcg atgaacgtga ccatgtagga cgagagttcc
     5881 tgctcgagat cgctcggctc gttgctctct cggtcgagcc cgaggctgcc ttcctcgagc
     5941 acctccgagt cgtgttcgag acgcggcgcg cgcagtcgtc ggcgatggct gtctccaatc
     6001 gcgctcttgg cgatgcggta gagccaggga acgagaaggt cttcattgcg gagtgcggga
     6061 agtgccgcct gcatccgcac gaagacctcc tgaagcacgt cttcgacgtc gcaactgggc
     6121 acgcgccgcg acacgaacgg ccggaggcgc tcctcgagct cacggtgtgc gcggcgcatg
     6181 tccgaagtcg acatcagctt cgagccttct cggtgcaaca ctccggcccg cagcatttct
     6241 gctccgcacc gggcttgatg gcttcgatgt tcgcggaagc aatgaaacgt tcgacgcccg
     6301 agcccggcat ccagtccttg atgaactcac cgctctcggc gcgaggagtg atgcggatgg
     6361 ccgtgaatcc ggcgtcgagg agcatggcct cgagttcggt gacgagcgcc gcgccgccga
     6421 cgcaggcgct caaagatgcg gcggtggagc gaaactcagt cgggatcgga gcgagcgcga
     6481 cgacatccga gatggcgaga cgcccaccgg cacgtaggac gcgaaaagct tcgcggaata
     6541 cgtccggctt gctcggggac aggttgatca cgcaattcga caggatgacg tccacggtcg
     6601 agtctgccac cggcaagcgc tcgatctctc ccagacgaaa ctcgacgttt cgcgccccca
     6661 gcttgcgggc gttgtcgcgc gccttcgtga ccatttgcgg ggtcatgtcg acgccgatca
     6721 cgctgccgct cgggccgacc tgccgggctg cgaggaagct gtcgaatccg cccccgcttc
     6781 ccagatcgag caccgtctcg ccgggcttca ggcccgcgag cgcttgcggg ttgccgcagc
     6841 cgagccccat gtcagccccc tccgggacgg cggccatttc ttccttcgag tagcctagcg
     6901 cggcgctgct gccgggcttc cccgcgcagc aactcggcgc acatccaacc gcgcccgcgg
     6961 tgtctgcgac ctttccgtag tgctcgcgca cgctgctcct gatttcgtct gctgtttcgt
     7021 gactcatcat tacctccaac cttgaagacg tcggtcgatg gaggaggacg caggaaatcg
     7081 taccgggcgg tgtcgggggc aaccgtcatc gtcgcgaccc gaacctccgg acccccattg
     7141 acggcgagga gctcttcatt catcgtcact ttacgatgaa tcagagccag gcggcgcagt
     7201 gttgccccgg agccgagcaa ccgggaggcg agcaggacgc tctggagctc cgcccggtag
     7261 aaggggatga cgcggacgag gagcttgcga acttggccaa ggcgctgtcg cacccggctc
     7321 gggtgaagat cgttcgcctg ctcgaacgga agaattcctg catttgtggg gacctggtag
     7381 aggagctgcc gctcgcacaa tcgaccgtct cccagcatct caaagtgctc aaggccgcag
     7441 gcttggtgcg aggtgaggtg gacgggcctc gcgtttgtta ctgcatcgaa ccccgcacgc
     7501 tgcgacggct gaaggcgctc gtagggggac tctgatgctg ccctctgtct atcgtaaata
     7561 cacgatgaaa ggactggaga tgtctataac cattcgcgtg ttcgaccccg ccatgtgttg
     7621 ttcaactggt gtctgtggcc cgagtgtgga cgcgagcctg gcgcgcttcg cggccgatct
     7681 cgagtggctt cgcggccaag gcgtaacggt cgagcgcttc aatctttcgc agcagcccgc
     7741 cgcgttcgcc gagacggcgg tggtccgaga agcgctccaa cgcggcaccg aggtgctccc
     7801 gttgatcctc gtgggcgacc gcattgcggt ggaaggcgcc taccccgcgc gcgagacact
     7861 cgcggccctg gccggcgtcg tcgtcaagaa gctcgatgtc gtggcgggcg ctgccacgag
     7921 ttgctgcggg ccgagcgcgg actcgaaatc gggaagcggc tgctgctgaa ggggctgcca
     7981 tgaatctcat tgacgcggct ccgcgcaatt tgttcttcac gggcaagggc ggagtgggga
     8041 agacgagcgt cgcttgtgcg actgcggttc gcctcgcaga acagggccgg cgagttttgc
     8101 tcgtcagcac cgatccggcc tcgaacctgg acgaggtgct cgagaccgaa ctcgggggcg
     8161 agccgcgcgt cgtcaccggt gtgcccaatc tcttcgcgct caacctcgat ccggaggcgg
     8221 ccgcgcatgc atatcgagaa cgcgtggtgg ggccctatcg cggcctctta cccgaggccg
     8281 ccgtgcgcag catcgaagag cagctctcag gagcgtgcac cgtcgagatc gccgccttcg
     8341 acgagttctc gaagttgctc ggggacgcga gtgcgaccgc gagcttcgat cacgtcgtgt
     8401 tcgataccgc gcccacgggc catacgctgc gcctgttgga gctccccgcc gcatgggctg
     8461 gatttctgga gggcaacgcg ggcggcacct cttgccttgg gccgcttgcc ggactccagg
     8521 cgcagcagtc gctctatcgt gcgacgcggg cggcattggt cgacgccgag cgcacgttgc
     8581 tcgtgctcgt ggcccgtgcg gaccgcgccg cgctcaaaga agcagagcgc acccgtactg
     8641 agctcgcggc gctcggcatc acgcgacagg tcctgatctt gaacgcgttg ttcgtggctt
     8701 ccgatgcgcg tgacctggtc gcggtgggga tgcagcgcca gggggagcga gcgctcggcg
     8761 aaatggcgcc cgcgcttgcc gcgctcgaac gagtcgagct gaggcagttg ccctttggtc
     8821 tcgtgggagt ggcggctctc cggacgctct ttgcgggagt agatgccggg tcagagtcag
     8881 cgtcgtaccc gccacggcaa tcgagcgaga gttctgcgag ggggctgggt gcgttgttgc
     8941 ccgagctcga agcggcggga cgcggtgtga tcctcactat gggcaagggt ggggtgggga
     9001 agactagcgt tgccgtcgag atcgcggtcc atctcgcgca gagaggtcac tcggtccatc
     9061 tcactacgac ggatccggct gcccatgtgg cggatgccct gggctcgcct gtcccggggc
     9121 tgcaagtcag ccgcatcgat ccggaggcgg agactcgaag ctactgcgac gaggtgatgc
     9181 gcactgccgg cgcgaacctg gacgcggcgg ggcgcgcgct gctcgaagaa gacttgagga
     9241 gcccctgcac ggaagaagtg gccgtgttta gggcgttcgc tcgaaccgtc gagcatggtg
     9301 aacatggttt cgtcgtgatc gacacggccc cgactgggca cactctacta cttctcgacg
     9361 ccgctgaagc ataccatcgt gaagtcctgc gctcggcggg cagcgccccc gaagaggttc
     9421 gtcggctgct gccgcggctc cgtgatccgg agtttacgaa ggtgctttta gtcacaattc
     9481 ccgaagcaac acccgtccac gaggcccgag ctctacagcg cgatctggag agggcgggca
     9541 tccacccctt tgcgtgggtc gtgaatcaga gcctagttcc gctgcgcgtc acggacccga
     9601 cgctggtaca acgccgtgag aacgaacggc gctttctcga agaggtccgg gagctttcgg
     9661 tgcgcaacgc aatcatcccg tggctcaccc ctcgcctggc tgaacagcgt tcggaactcg
     9721 cgctcgatac cgcccgagtc gccggaggta tgtcgtaacc gtggccgcct cgaactcaat
     9781 ggtcatgccg gaggcgagcg tcggagttgc ccgcaagctc tctttcctgg atcgatatct
     9841 gacggtctgg attttcgccg ccatggctct cggcgtgggt ctcggctacg tcgctcctgg
     9901 gtttacggcg gcgctcgacc gaatgagcgt cgggactacg tccgttccaa ttgcgattgg
     9961 gctcattctc atgatgtacc ctccgctcgc gaaggtgcgc tatgaggagc tgggccgagt
    10021 cttccgaaac acgcgggttc tgtcgctgtc gctgattcag aattggatcg tgggcccgct
    10081 gttgatgttc gggctcgcgg tcctctttct gagagacaag cccgagtaca tgctggggct
    10141 catcttgatc ggcctcgctc gctgcatcgc gatggtcatc gtttggaatg acttggccca
    10201 gggagacacc gagtattgcg cggggctcgt cgcactaaat tcactttttc aggtgctatt
    10261 tttctcgctc tacgcgtacc tgtttgcgac gctgctcccg tcctggctcg ggctggaagg
    10321 cgcggtcgtg cacatcacga tcggggagat cgccgagagt gtggcgatct atctgggtat
    10381 tccgttcgcg gccggcttcg tctcgcgccg cctgctcgtc gcgcgcaaag ggcgcgattg
    10441 gtacgagacg cgcttcgtgc cgcgcatcgc accgattacg ctgattttcc tgctcttcac
    10501 gatcgtggtg atgttttcgc tcaagggcga gaccatcgtg cagctcccgt tggatgcgct
    10561 gcgcatcgcg ctgccgttgc tcgtctactt cttgctgatg ttcgcgatct cattggtcat
    10621 gagcaagcgg gttggcgcaa cttacgctca atcggctacg ctctcgttca cggccgcgtc
    10681 caacaatttc gagcttgcga ttgcggtggc gatcgcaagc tttggaatcc atagcggtgc
    10741 ggccttcgcg gccgtaattg gtccactcgt cgaggtgccg gttctgattg ggctcgtgaa
    10801 cgttgcgctt tgggctcagc gaaggttctt cgcgcccgcg cctcggagcg gactcggttt
    10861 aaactcctag catcgattca gtcgaacatg actagcaact ccaaaagcat tctgttcttg
    10921 tgcgtcgcta actcggctcg gagccaaatg gcggaagggc tcgcgcgggc tctgttcggg
    10981 gagcgtatcc ccgtcgagag cgcgggcagt gagccgtcga aagtgaaccc ctatgcgatt
    11041 gaagtcatgc aggagagagg gattgacctt cgagcgcacc actcgaagtc cgtgcaaacg
    11101 gtcgacccgc agcgagtggg tatcgtgatc actctatgtg cggaggaggt ctgcccggtt
    11161 ttcctgggtc gagccgaacg tctccactgg cccattccgg atcccgcgag ccaagatagg
    11221 agcatttcac gcgaagagat gctctctcgc tttcgaacgg cccgagatac cattcagagt
    11281 aaactcgaac agttccagaa ggaaagaggc cttggttgaa acaaataata gtgcctgcgg
    11341 ccgaaagcga cctcggggcg gtattgctgc tgctccagca ggtttctttg ccggtcgcgg
    11401 gggtagcgga tgagttccct ggggattact gcgtagcgcg gctcaacgac gaggtggttg
    11461 gggtggcggg gctcgagagc tacggcgagt ttgggttgct gcgctcggtc gccgttcatt
    11521 catcccggcg aggcagcggc ctcggtcgac tattggtcga agaccggctc gtcgccgcga
    11581 gagcgcgcga gctgacgtcg gtctacctcc tcacgaccac tgcggcagca tacttcgctg
    11641 tttttggctt caagatcgca tcacgaggag ctgcgcccga gaacatccgc gcttcgttgg
    11701 agtttcaatc catttgtcca gcttctgcgg tgtgcttggt gcgtgacttg cgggcaaatg
    11761 aataacggca agtcagcgtc gaatgagctg ccggggggcg cggcctcttc atgggacttc
    11821 gatcacgttt ggcgtggcgc tctgattgcc aacgaaaacg aacaaaacgt ggcgcccgct
    11881 aggtgcgggg ttcggggcgc tgaggctcgc gagaggaacc gaaaaggtgc caatcaggtc
    11941 acgtttatcg tgctgcaagg tcaatcgata ttgacgagtc agaactcccc cggatgagcg
    12001 caatcgagcg agcacgatcg ttagaactgg ctcgctcgaa gtggcgattt ctcgaaatgc
    12061 cccgacgaca gctgcgcgct cggaaactcg cacggtgccc gtagcgcttg gcgcagcagg
    12121 gatgggcgca tctccagctt cagccaaagc gagctgagcg cttgccggat ctggggccat
    12181 tggacctgcg gcgtccttga tgcggagttc ggttgggccg gtccaggcgt ccgcgaaagt
    12241 agccacgacg aagtagtcgc ctgcgcccgg gtgggcccga ccataagcga gcaggtcgta
    12301 ctgtttctgc tcgatgattc gggcgcgatc gctcgcgagc tccgcatcgg agggcctccc
    12361 ccgcggtggg ctctgaggta gcatcatggc atcggggtcc accttcgcga tcgaaatcaa
    12421 gcccggttgg tcgcgccgga ttaggagcac ttccagggca ttatcgagca ccccttgatt
    12481 gtcccagtag gtgcgcgcgt ccgcattcaa cgtaacctga atgggaattg gacccgcgga
    12541 aggtgcttcc ggggggcatt tcaaggtcac ggcggcagac gcgcgctgcg gcgccgccgt
    12601 gcccggcgcg ccaacggctg cggaaggagc cgaagactcg cgtctttgtg cgtctttggc
    12661 gcaactcgag agcaggaaag cgagtacgcc ccaacgtaga accctgctct tcgagctaat
    12721 cgacatcatc ggtcgccaaa ataacatcgg ttggagggac cagcgcgctg cctaacacgg
    12781 cgaagaacag cgaggtggcg aagccagagc gctcagtgga atcacggctt cggcgttccc
    12841 ggtcgctccg atggtttgag catctggtgc cctccgaaat cgaatcagag gcgtcttcca
    12901 tgtccacgct tgccggagtg aacgcgaagg cagtacgctc cccgatccag cccgaccggc
    12961 gcggcggctt gatgggtgag ggggaccttg gtacgctgct ccggcgtgag tgacggccgg
    13021 tcccccgctg ggcgcctgaa tcaacccgga tcaaactgag acacgatgca gcgcccgcca
    13081 gctccgaact ccgatccgcc gagctcgttt ttacccggca cggtgatcga cggtaagttc
    13141 gaggtgcggc ggctgttggg ggtgggagcg atgggagccg tcgtggaggc gactcacctg
    13201 cttcgacgcg cccgtgtggc cctgaagttc atgagtgagg cggtcgcgca cagccccggc
    13261 gtggtcgagc gcttcttgaa cgaggggatc gccgcgtcgc gcatcaacaa cgagcacgtg
    13321 gtccgcgtgc tggacgtgag ccgcttgccg aacgggcaac cctacctcgt gatggagttt
    13381 ctcgaggggg aggacctgca gagcttgctg cagcgagaag gtcgtcaggg cctgccagac
    13441 gtggcgcgcg ccgtgcactt ggttctgcag gtgcttcgcg ggctgcaagt ggcgcatcac
    13501 gccggcatca ttcatcgaga tctgaagccc gccaattgct tcgtggtgac ggaggacggt
    13561 gagccagatt tcgtcaagat cgtggacttt ggcatcagta aggtccagca gggggacggc
    13621 acgctgaagc tcacgacgga gggggcatcg atgggcacgc ctctctacgt gtctcccgag
    13681 caggcgcgtg atgccatgaa cgtcgacgcg cgctccgata tctacagcgt ctcggccatg
    13741 ctctacgagc tgctttgtgg gcgtacgccg ttcgaagcga cgacgctgat ggggctattc
    13801 atgcagctcg cgaccacgat gcccgagccc gtgggcgcgc gccgcccgaa tctacctgcg
    13861 gggttatcgg aggtggtgca gcgcggtctc gcaaaacagc cgagcgagcg gtttcagtcg
    13921 gcggccgaaa tggctcaggc tttggcaccc tacgcggacg cgcggagtga agggctgttg
    13981 ctgaaactcg ctcaacgccc gtcgctatcg gcgagtcgcc cgtcatcccg cccgccttcg
    14041 cgcgcaggag gctctgcggt ttccgtcggg gatcggacgc aacgctccaa gggtatcggc
    14101 tcgcagcctc cgcattcgcc ctcgtcgaat gcgctcacca tggatgcgcc cgccgccgcg
    14161 ccttcccgag cgcgggtgcg actggtgctg ggttctttgg cggtggtgct gctggcggct
    14221 gcggccgccg caggcttgcg atggttcaag agttcagcag aaacgactcc cgccgcttcg
    14281 gctacctcga gcccctcctc ctctgccgcg cccgagtcag ccccacgtag cgggcagccg
    14341 acggtgatcc ccgtaccttc cgtcgaggtg agcgcgcagg tccaggcctc cgggtcgagc
    14401 tccgccacca tgcccggcgg aacaggtcct tccgctccgc cgcccattcg ggcaaagtcg
    14461 gacccgagtg tctcggcttc cggaaagaac ggatcgaccg gagccgagac ggttcgtccc
    14521 aaaggtccca ggccttcgct cggcaagatt ggcgttcaag attgatcgac ggttcaccgt
    14581 actgcccggg ctggagatga agatgttctc cccttccaag cattcgagtt tggttttgtt
    14641 gtctgcgtgg tcgctctgtt tttcgagtgc cattcctatg gcgcaggccg cacctccggc
    14701 tcaggccgcg ccgaaaaccc agagccagcc caaggcgccg gccgcaccga aggtgcaggc
    14761 caaggctcct gctgcgccca cggttcggga agaactgtcg gcagacgccc agcgtgattg
    14821 ggatgccgca cgagagctct acgacgcacg agattaccgc ggggcgttgg tccacttcga
    14881 gaaagcctac gagctgagcc gcaacccgcg ggtgttgttc aatgtcggcg tgtgttggaa
    14941 agatctgact cgctacgcgc aggcgattcg cgtctgggag cgtcagctcg agttcggcgc
    15001 gcgcctgtcc ctcgaagatc tcgagcgcac caaatcggcg atcgcggccg cacgcccctt
    15061 cgtttcgacg ctcacgctcc aaagcgatca acccggcgcg gtgatcctga ttgacggggt
    15121 cgaagccggg acgactcccg tgaccgaggg acttcctgta gatgtggggc gtcacacggt
    15181 gaccctcaag aaggatggtt ttgcgccgag cgaggccgcc gtcgacgtga tccaaggcac
    15241 cccggccgcg ctgaccctga acatgactcc actcgtcaaa ctgggcacgg tcgcgattac
    15301 cgtagtcggg ccgcgcagcg ccaccgtgtt cgtggacggg cgtgagctcg gtccggctcc
    15361 cttcaccggg actgtccaag agggtcccca cacgttcgaa gcgcgtgcgc ccggatactt
    15421 gccggctcgg cagacgaccg agattcgcta cgcggagcga agcagcctga ccctcgcgct
    15481 cgcggaggct cgcaacgagg gcaaggtccg gatcgtgacg gactacccgg acgccgcgat
    15541 tgccgtcgac ggcgaggtca agggcagcgg agcctgggag ggcttgttgc cggctggagg
    15601 tcaccagctg cgggtccaga agtccggctt caaaacgcac gtgaccgaac tttcgttgag
    15661 cgccgggcaa cagcgatccc tgcaggtgca tttggaaaaa cagcagtcct ggctatggtg
    15721 gaccgtgggc atcgcgaccg tcgtcggtgg cggaaccgtg gccgctatcc tgctctctcg
    15781 cccaaccgat acctcgcccg ttacgggcac actgggcacc ttctgaactc ccccttaccg
    15841 agcgacgcag catgaacatg ggataccaaa ctcgcttctc gcatgaacgc ttgctccgag
    15901 ggctgggatt cacggcaagc cttggattga ccgccttggg gagcctggga ttgggcgcct
    15961 gcagcggaga tcgcccgacc gagatcgtcg cgggaatgat gacccagttg cgtgtgcccg
    16021 acgacattcg agccgttggc gtggtggtgc agcaaggcgg ccgcgtcgag ttctgcgaga
    16081 gctactccgt gaccgacggc accgcctcgc tgcccgcgac gattgggtgg ctcaatgagg
    16141 ggcgttccgg caaaggtcca gtcacggttc aggtgctcgg gctccgcacc gaagactcgg
    16201 ccttcaccac cgactgcgtc accacgcgcc cggagtctct cgggaaacaa gtgcgggtca
    16261 tccgccggcg tagattgtcc ttcgtcgacg atcacattac ctatctgccc ctgccgctga
    16321 aagactcgtg tcgagatatc tcggattgca acgaagacga gacctgcgtc gggggcaagt
    16381 gcgtggcgat ggacatcgat gcgaccacgc tgcctgatta caaagattct ttcgtgttcg
    16441 gcaataccaa cacttgcttc gatggcaacc tctgcatgcc caagggcaag acgttgcccg
    16501 tcagtttgac cgatctgaac gactgcagct tcgaagttcc ggtgcccgag caagcccaga
    16561 ttcccggcgg cgagctcaac gtgcgtatgt tttacgagtc cttcggga
//
LOCUS       DASTKU010000500         4955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_541726, whole genome shotgun sequence.
ACCESSION   DASTKU010000500 DASTKU010000000
VERSION     DASTKU010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541726"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..1065)
                     /locus_tag="VFQ61_17305"
     CDS             complement(187..1065)
                     /locus_tag="VFQ61_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956271.1"
                     /translation="MAKSGIVLRQNLPEFAIVTEYYNRAKHEPTRAGDLPISRVDVFA
                     NTFKSLCDLILAKRPTDVVVVAHGTPDLGLIMPIAPATNVSTGYAMPDLVKLVNEVES
                     AGSNKVDSDRVSGMANDWGISEASALGLARTCAKIRKEISKSLRVHIRGCDIGAKAQH
                     LRAMQRVFNAELVSAPDCAMFYVRVQPGIVKDVDEYANRTPVHGRRSLYQDPENPKRG
                     KLLLDIEYLGTKATSFSAVAKQAHVGAWADFLLERPGGAAGSFALAGLWPHDNTLLYW
                     LPHETGYAGRITDVSR"
     gene            complement(1113..2324)
                     /locus_tag="VFQ61_17310"
     CDS             complement(1113..2324)
                     /locus_tag="VFQ61_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956272.1"
                     /translation="MHRALGLTPLGIGAVWLVAGCVTAAAPPPPNARSAPRGDSAEPK
                     VTAQPKATTQPKSPELPPDTAVPAGPNIGTTPNAQKPCEEASRSRVPPAVVAGPSAAA
                     DATTCLARIARGSLRDIQISIAGQPALHLNVTGAPLEITPLATSDGAARVQGRGDLVF
                     EGLAYVERWALREPATLVPNVLRAGTGLTLKPVRRVGAALEGTAELADLEISLVRVAC
                     AQLKPFLPGSARTPRPVVTAGLRWQARSAGLKLRIAPQGADGVELKGPLAFSGGQQRG
                     GFRELSARFDDGSWIRGWAPEEELVSVPPGEFGGGYGEGLSLCRPGCAGRGPEPVHFR
                     GRARVSANAAVHAAPGGAVWARTGRDLHAEVVQRDAGGWVELVKLPTVEEDEGCHSMR
                     HAFVAASAVRF"
     gene            2749..3033
                     /locus_tag="VFQ61_17315"
     CDS             2749..3033
                     /locus_tag="VFQ61_17315"
                     /inference="COORDINATES: protein motif:HMM:NF015640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HET9956273.1"
                     /translation="MAKPSEPPSSIPEDVARFAEAQVNAGRFSSVGEVLRAGVEAIRL
                     RDELEAERVALLRAAWDHGIKSLKDHGPQLENDADFEAFLDECEADSARQ"
     gene            complement(3696..4013)
                     /locus_tag="VFQ61_17320"
     CDS             complement(3696..4013)
                     /locus_tag="VFQ61_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956274.1"
                     /translation="MMQRILLLFAHLSSLPLLAFWVAIGVRVLRDPGNHVGWSPLWAL
                     PFFACLAGWCLFTWIWTKRRSIAALGLFAAASLLGVYLLDRLAILMSYESWIARGMPA
                     RPF"
BASE COUNT          933 a         1598 c         1550 g          874 t
ORIGIN      
        1 gcggtgaagg agaacgcggg gagggcgggg gtggaggggt gagggggctc aagtcgcggg
       61 ggcgcaattg ccgctgtacc acgggctgtc attccaattg cgttattccc tcgcggctcc
      121 cacggatcgc ttcgagatct ctcgggctgc acgcgcagtg aagagaggcc cacctctcag
      181 ggacccctag cgcgagacgt cggtgatccg acccgcgtag cccgtctcgt ggggcaacca
      241 gtagaggagc gtattgtcgt gcggccacag accggccaag gcgaaagagc ccgcggctcc
      301 acccgggcgc tcgagcaaga agtcagccca ggccccgaca tgcgcttgct tcgcgacggc
      361 ggagaacgaa gtcgctttcg tgccgaggta ctcgatatcg aggagcagtt tcccgcgctt
      421 cggattctcg ggatcttgat agaggctgcg ccgcccgtga acgggcgttc tattcgcgta
      481 ctcgtccacg tccttgacga tgcccggctg gacccgcacg tagaacatcg cgcaatcagg
      541 cgcgctcact agctcggcgt tgaagacgcg ctgcatggcg cgcaggtgct gggccttcgc
      601 gccgatgtca caaccccgga tatggacgcg gagtgacttg ctgatttctt tgcggatctt
      661 cgcgcaagtt ctggccaagc cgagcgcgct agcctccgag attccccagt cattggccat
      721 cccggagacc cgatcggagt cgactttgtt cgagcccgcg ctctccacct cgttcaccag
      781 cttgactaag tcgggcatcg cgtagcccgt cgagacgttc gtggcggggg caatgggcat
      841 aatcaagccc aggtcgggcg tgccatgcgc gacaacgacc acgtccgtgg gtcgcttcgc
      901 caggatgagg tcgcataggc ttttgaaggt gtttgcgaac acatccacgc gcgaaatggg
      961 cagatcgccg gcccgcgtgg gctcgtgttt agcgcgattg tagtactccg tgacgatcgc
     1021 gaattcgggc agattctgcc gaaggacaat gcctgattta gccatctaca caccaactcc
     1081 ccgcgactct cgcgccgcgc acacccaaga aatcagaatc ttaccgcact cgctgccacg
     1141 aatgcatgac gcatggagtg gcagccctcg tcctcctcga ccgtgggcag cttgactaac
     1201 tcgacccaac cgccggcatc gcgctgcacg acctccgcat gcaagtcgcg acccgtcctc
     1261 gcccagaccg cgccgcccgg cgccgcgtgc accgcagcgt tcgcgctcac ccgcgcgcga
     1321 cctcgaaaat ggactggctc cggtccacga ccggcgcagc ccgggcggca caacgagagg
     1381 ccctcgccgt agccgccgcc gaattcgccc gggggcacgg agacgagttc ctcctccgga
     1441 gcccaacccc ggatccaaga cccgtcatcg aagcgtgcgc tgagctcgcg aaaacctccg
     1501 cgctgctgac ctccgctgaa cgcgagtggc cctttcagtt caaccccatc cgcgccctgt
     1561 ggggcgatac gcagcttgag gcctgcgctg cgcgcctgcc agcgcaaccc ggctgtgacg
     1621 acgggccgcg gggtgcgagc gcttccgggc agaaaaggct tcagctgcgc gcaagcgacc
     1681 cgcaccagtg agatctccag atccgccagt tcagcggtgc cttccagcgc ggccccgact
     1741 cgacgcaccg gcttcaaggt gagcccagtc cccgcgcgaa gcacgtttgg cacgagcgtc
     1801 gccggctcgc gcaaagccca acgctcgacg tacgcgagac cttcgaacac taggtcacct
     1861 cgtccctgca cgcgcgcagc accgtcgctc gtcgcgagag gtgtgatctc caacggggcg
     1921 cccgttacgt taaggtgcag cgccggttgg ccagcaatgg agatctgtat gtcgcgcagt
     1981 gagccgcggg cgattcgggc cagacaggtt gttgcgtcgg ccgcagcaga tgggcccgcc
     2041 actactgccg gcggaacgcg agaacgcgaa gcttcttcac acggtttctg cgcattcggc
     2101 gtggtgccga tattaggccc agccggaaca gccgtatccg ggggaagctc tgggcttttg
     2161 ggctgtgtcg tggccttcgg ctgtgccgtg accttcggct ctgccgagtc acctcgcggt
     2221 gcggagcgag cattcggggg gggcggagcg gctgccgtca cacaacccgc gacgagccag
     2281 acggcgccga tcccgagcgg ggttagcccc aatgcgcggt gcacccagat cactttcgta
     2341 agctaacgaa gccgccctgg taacgcaagc ggccgacacc caacgctcga ccctgtcatt
     2401 tcggaaccgg gggctgtggt gctgccgctg aggtgagtag attgctgatc gcgtcggcca
     2461 gttgccggcg agctgatcga gctctcgcaa gcgctgcata tactcgagat agatttgacg
     2521 gtatcgaccc gctcggcctt ggcgatgaac gagcgacgct gaagagctcg agctcttgac
     2581 gaagccgctc gcgcgaggca cgcgaaccga tcgcgttcca gctccagcgc tgagatgcgc
     2641 gtacgggaat cgccgtgggt cggggctgag tcagagcttc cgtggtgcct aacgcagttg
     2701 tctcaacgtc aacttctgcc acaatcatcg aagcttctag gactccgcat ggccaaacct
     2761 tccgaacccc cctcctcgat tcctgaggac gttgcccgct tcgctgaagc tcaggtcaac
     2821 gccggtcggt tctcgtccgt tggagaggtg ctacgcgccg gggtcgaagc catccgtctg
     2881 cgcgacgagc tcgaagctga gcgcgtcgcc ctgctccgtg ctgcgtggga ccacggtatt
     2941 aagtcgttga aagaccacgg gccgcagctc gagaacgacg ctgacttcga ggcgtttctc
     3001 gacgaatgcg aagctgattc agcacggcaa tgaccgtgcg cgtagtcctg actgaagcgg
     3061 ccaaggccga catccgcacg attctgcgcg agatccgaga aaaattcggg ccgatgcagg
     3121 tgccgcgcta ccgagccctc atcgccgaag ccagacagcg gttgcagcga atgccgactt
     3181 tgggtcatcg tcgcgaaggc ttgccgtcag aagctcgctt gctccacatc agtcagccag
     3241 ggcgctccgc ccagccactt cttcctgttc cgcgtgaacg acaccgaggg gcctcatcga
     3301 ggttattcgc gtcctgcacg aggcgacgga tattccgagg cattggtagc cttcgcgttg
     3361 aaccgcagcg cgtcagtggg acatccacat gctacgcatc actgccgatc gaagtcagcg
     3421 cacgccctcc tgggcgcccg cggggcagtc cgatgacata ggcgatctgg acggttggtc
     3481 tgttccgttg agtggacgcg cgtgagcgac tatccccgta gggcgctgcg gttctagtcg
     3541 cgagggtcca cgacttgccc ggtgggtacc ggcggcgcgg gaatggagaa gacggtcgcg
     3601 cccgaatcag ccgagtcgcg gcgagaacgc gccagggagt ttgtgccatt cgggctgctc
     3661 acatcgtgac ggctgattgc cgcgatgcac tgggctcaga acggacgggc tggcatgcca
     3721 cgcgcgatcc aagattcata ggacatcagg attgccaagc gatccagcag gtagactcct
     3781 agcagcgacg cggcagcgaa cagcccgagc gcggcgatag agcgcctctt cgtccagatc
     3841 cacgtgaaca agcaccaacc agccaagcat gcgaagaatg gtagagccca gagcggactc
     3901 catccgacgt gatttccggg gtcgcgcagg acacgtacgc cgatggcgac ccagaacgcg
     3961 agaagcggga ggctggacag gtgagcaaaa aggagcagga ttcgctgcat cacggcgacc
     4021 ctcgaattca agtgtacact cgaccaagca tacgactgcc gttgccgagt acgccgttct
     4081 ttccgcgatt gaacgcccat agacagatgt cttggtcagc gggactagac tcatcgtacg
     4141 acgaccaacg cttagagaac gacgaatagc ccgtctggcc cagggtgcgc accttctggc
     4201 tagcggcgaa aacgcttgcc ggacaccatc cgcgcttgcc ggcgataccg gtgttgcagc
     4261 ccatcagcat cagcgctcct ttttcgtgcc agggcaacgg ttcgagcgtg gatatctccc
     4321 cgcgactcac cgttccgtcg tcagaagagg agggccgaaa ctccaagccg ttggcgtact
     4381 cggcacgcct ccgctactaa ggaccagacg cgtcggaatg ccttctcaga ctgcacttcc
     4441 caactcagga tttcgtcccg cgcggcattg aaccccgcca gcaagggcgc cttgcgacgc
     4501 caagtctctg cagccctagc aaacgcgcga tctggaacgg agtactcgat cgtgaagaag
     4561 atgcgcggct ccccttaggt tgcgctctgt gggccgagtt caggcaagta gtacatcgtg
     4621 gaattctaga agcgcgagcc ttcgcgagca actacttgtg aggtcatcag ttgatgaagt
     4681 ggtgtgtgct tactggttcg ggttctccgt cgaggccacc gcaaagccga ttgtccaccg
     4741 ctcccgcgcc gaatgaccgc caagttgcgg gtcgcgctcc gcgaaccgag ccgcagaacc
     4801 agcccctcgc ccccaatgcc gaatcttaat ctcgagctgg aatagcggtt acgacgtttc
     4861 tcaaagcgtg gagccgcaga gggcctctgg tgagtttgcg cacggtctcg gttgcgctct
     4921 cgactgccgc cttcgagcaa gcccgctggg agatt
//
LOCUS       DASTKU010000501         2937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_541770, whole genome shotgun sequence.
ACCESSION   DASTKU010000501 DASTKU010000000
VERSION     DASTKU010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541770"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="VFQ61_17325"
     CDS             complement(<1..1505)
                     /locus_tag="VFQ61_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956275.1"
                     /translation="MRRRLEPCDRTEVRRRFVRGRVEPRGVVWFGLRSFWGHLRHFLA
                     SGIATEDIDSRHWMTPDDPTQLVQRVAEILGGKPNATNLLESLDRDLFIDYVSDTGDD
                     VSVSQALANLLVAEYDLPDPRCPTERIEAPRGELLLFGGDTAYPVATALEITNRIIVP
                     FNRAIEAQPDDKPRVLLGIPGNHDWYDGLDGFGRLFRRRDEEPTDSRPTLFGISPRML
                     QHYTEWARELVRGGKVEKPRALVLSGYTPVQDASYFVLPLTPDIHLFAADRQLKQIDP
                     RQRRFMREWYGNHPEVAPWVLLPDPLYAFGRPSPTGTRTVESLGLDFGARDHFLLSGD
                     VHHYERSKEDRLLHVTAGGGGAFLHPAPMFEGRLRADVRWPNATQSRALLRQVPWKVA
                     RGRSGFLPHLSLAALYGLALAASTAYDEQPALLPLSSAALCLIVTLVYRAIGGLRNAA
                     RRVLPLAFGAGLLTACIPQVLAFGLERAALGSHVMLSRWVIGLLSLLAAAFV"
     gene            complement(1583..2863)
                     /gene="hflX"
                     /locus_tag="VFQ61_17330"
     CDS             complement(1583..2863)
                     /gene="hflX"
                     /locus_tag="VFQ61_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET9956276.1"
                     /translation="MSRPKAALLAVKLPDEENTEFEASLAELARLVTTLGYEVSATLT
                     QHRAQYTPATLIGSGKLQELVELTGYGENEGELEERRPGEHIELVAVDHDLTPTQLRN
                     LEKACGAQVLDRTGVIIEIFHRHAKSKKAKIEVELARLAYEAPRLRESPRGKEKQQGR
                     GAGEAQLELDRRKIRDRMAELREELELLQREDRVRRAQRHDARRVALVGYTNAGKSSW
                     MRALTGSQVLVEDKLFATLDTTVRALHPEVTPRILVSDTVGFIKKLPHDLVASFQSTL
                     DEALEASLLLLVADATDPGFREQLAVTQQVLGEIGATHVPSRLLLNKIDRLAETQLTE
                     LAHEFPDAWLASARRPPDVARIHGQIVDFFAADNIEGELFVPYDRQRLIAELHQVGHV
                     LQVDYQDAGVRVRVRADPQTLDRIRDLCAAAQSD"
BASE COUNT          514 a          941 c          952 g          530 t
ORIGIN      
        1 acgaaggcgg cggccaatag cgaaagcagc ccgattaccc atcgggatag catcacgtgg
       61 cttccgagcg ccgcacgctc caaaccgaaa gccagcacct gcggaatgca tgcggtgagc
      121 agccccgccc cgaaggcgag cgggagtacc cggcgggcgg cattccgaag ccccccgatc
      181 gctcggtaga ccagcgtcac gatcaagcac agcgcggcgc tgctcagggg caaaagcgca
      241 ggctgctcgt cataagccgt gctggcggcg agggcgaggc cgtacaacgc ggcgagcgac
      301 agatgcggca agaagccgga tcgtccgcgc gccaccttcc agggcacctg cctcaacagg
      361 gcgcgactct gggtcgcgtt cggccatctc acgtccgcgc gcaaccggcc ttcgaacatg
      421 ggtgccgggt gcaggaaggc gcctccgccg cccgcggtga catgcagcag gcgatcttcc
      481 ttcgagcgct catagtgatg tacatcgccc gataacaaga aatggtctct tgccccgaag
      541 tcgagcccga ggctctcgac ggtgcgggtt cccgtcgggc tcggtcgccc gaaggcgtag
      601 agtgggtccg gcaaaagtac ccacggtgca acctcgggat ggttcccgta ccactcgcgc
      661 atgaagcgcc gctgtcgcgg gtcgatctgc ttcaactgcc gatccgcggc gaacaagtgg
      721 atatcggggg tcagcggcaa gacgaagtag ctggcgtctt gtacgggcgt atatcccgag
      781 agcacgagcg cgcgcggctt ttctacctta ccgccgcgca ccagctcacg cgcccattcg
      841 gtgtagtgtt ggagcatccg cggcgagatc ccgaacagcg tcggacgaga atccgtgggc
      901 tcctcgtcgc ggcgtcggaa cagcctgccg aagccgtcta gcccgtcgta ccagtcgtgg
      961 ttacctggga taccgagtag cacacgcggt ttgtcgtcgg gctgcgcttc gatggcgcgg
     1021 ttgaacggga cgatgatgcg attcgtaatc tcgagcgcgg tcgcgaccgg gtaagccgta
     1081 tcccctccga atagcaggag ctcaccccgc ggggcctcga tgcgctcggt cgggcaccgc
     1141 ggatcgggaa gatcgtactc ggcgacgaga aggttcgcca gggcctggct gaccgagaca
     1201 tcgtctccgg tatccgagac atagtcgatg aacagatctc tatccaacga ctcgagcagg
     1261 ttggtggcgt tgggtttgcc gcccaggatt tcggcgactc gctgcacgag ctgcgtcgga
     1321 tcgtccggtg tcatccaatg ccgggagtcg atatcttccg tcgcgatgcc ggacgccaag
     1381 aagtggcgaa ggtggcccca aaaagaacgc agcccgaacc agaccacgcc ccgaggttcc
     1441 acgcgcccgc gcacgaagcg acggcgaacc tccgtgcgat cacagggctc gaggcggcgc
     1501 cgcaccttcg cttggttcgg cctggctgcg ggcggcgcgg cagcggattc agagaggtgg
     1561 ctggatgcgg cgatgctcgg aattaatcac tctgcgcggc agcgcacaag tctcgtatgc
     1621 ggtccaaagt ttgggggtcg gcacgcacgc ggacccgaac ccccgcgtct tgatagtcca
     1681 cctgcagcac gtgacccact tgatgaagct cggcgatgag ccgctggcga tcatagggga
     1741 cgaagagctc cccctcgatg ttgtccgccg cgaagaagtc gacgatttgt ccatgtatgc
     1801 gcgccacgtc gggagggcgc cgcgctgacg ccaaccaggc atcggggaac tcgtgggcga
     1861 gctcggtaag ctgcgtctcg gccagtcgat cgatcttgtt caacaacaaa cgcgacggca
     1921 cgtgggtcgc accgatctct ccgagtacct gctgggtgac ggccaactgt tctcggaaac
     1981 cgggatcggt cgcgtctgca acgagcagga gcagagacgc ttccaaggcc tcgtccagcg
     2041 tgctctggaa gctcgccacc aagtcgtgcg gtagcttctt gatgaacccc accgtgtcac
     2101 tcaccagaat gcggggcgtg acctcggggt ggagcgcgcg caccgtggtg tccagggtgg
     2161 cgaagagctt gtcttcgacc agtacttggc tgccggtcag cgctcgcatc cacgatgact
     2221 tgcctgcgtt ggtgtagcca accaaagcca cgcgccgtgc gtcgtgtcgc tgagcgcgac
     2281 gcacgcggtc ttcgcgttgt aggagctcga gctcctcccg cagctccgcc atccgatcgc
     2341 ggatcttgcg ccggtcgagc tcgagctgcg cctcgccagc gccgcgccct tgctgttttt
     2401 ctttgccgcg aggcgactcg cgtaagcgtg gcgcctcgta cgccaagcgc gcgagctcga
     2461 cctcgatttt ggccttcttg ctcttggcgt gtcgatgaaa gatctcgatg atcacgcccg
     2521 tgcgatcgag cacttgtgct ccgcaggcct tctccaggtt tcggagctgc gtgggagtca
     2581 aatcgtggtc gacggccacg agctcgatgt gttcgcccgg tcgacgctcc tccaactccc
     2641 cctcgttctc gccatacccg gtgagctcta ccaactcctg gagcttgccc gagccgatca
     2701 gggtggcggg cgtgtactga gctcgatgct gagtcagcgt tgcgctgact tcgtaaccga
     2761 gcgtggtcac gagccgcgcc agctcggcga gactggcttc gaattcggtg ttctcttcat
     2821 ctggcaactt gacggccagc agcgcagctt tggggcgact catgttcggt gcagagtctc
     2881 atccatggtc cgtgcctcgg caacccgctc tgcggtccga gtgtccgcgt catggtc
//
LOCUS       DASTKU010000502         6648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_318331, whole genome shotgun sequence.
ACCESSION   DASTKU010000502 DASTKU010000000
VERSION     DASTKU010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318331"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /gene="prs"
                     /locus_tag="VFQ61_17335"
     CDS             complement(<1..512)
                     /gene="prs"
                     /locus_tag="VFQ61_17335"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET9956277.1"
                     /translation="MNGALKIFSGSAHPELAKAICHYAGIPLGASHTHRFSNENLKVK
                     IEENVREQDVFVVQTSCSPVSEGILELLIMIDALKSASARRVTAVLPYFPYARSDKKD
                     EPRISITARLMADLLETAGADRVLSMDLHSPQVQGFFHIPADQLSAVPLICDHLAKSD
                     LSEAVVVAPDA"
     gene            complement(576..1709)
                     /locus_tag="VFQ61_17340"
     CDS             complement(576..1709)
                     /locus_tag="VFQ61_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956278.1"
                     /translation="MSPILPLWLLTVQPTPAQVVGDPGTPRYARAEGPAALLLSSASW
                     QRDLESFSSRWSVRFQIVSRPEPPNPAARTNAAEMDLADQIEAALDAARTALGSLDAD
                     AAERTLADIERKLDEHPELPQAAWLMAEHHYVEAELLERAAVVDRAGLKPTSTRQTEL
                     RSRARELRNDAEALEGIRAMPFREEKSKELGPPPMTGISLGIAGLRPRDEIEWDGALQ
                     SSALTTRPGKHHVRVLRGERLVWAGWIQVTPDQRVLSLPVSAPAPCSGEELMGALDGD
                     PAPIAAGATECPQWAIARMRGPLLEVALCRRAQCGRWYGVQTEPVAFRGPAQPTERRG
                     IPSWALYAGLGAGAALALGVVAVTSGWFDRNPPHETWRYEGLR"
     gene            1795..2556
                     /locus_tag="VFQ61_17345"
     CDS             1795..2556
                     /locus_tag="VFQ61_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HET9956279.1"
                     /translation="MQLDFVNRELTIKLVYYGPALSGKTTNLQALHKCAAPGASGRLM
                     TLETRDDRTLFFDLLPLTFKSQGSLAVRLKVFTVPGQVIHASTRRLVVQGADGIAFIA
                     DSRVVETQHNADSFLDLKHNLKEHGLVLKKMPLIIQFNKRDLPDVRKDDEIAQLAAQG
                     REPVYMAVATRGVGVVESFLGLLHLTWSALDFEHELSKKWGFDAYAFLGQVAEKLGDK
                     RPLREILRQNVGGSIDARPEAATAADKDTKQGAGR"
     gene            2553..3974
                     /locus_tag="VFQ61_17350"
     CDS             2553..3974
                     /locus_tag="VFQ61_17350"
                     /inference="COORDINATES: protein motif:HMM:NF021619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9956280.1"
                     /translation="MTQPVAARSLDELTLGVGVKLEDLLDREGLSEMLHSVYDLFQLP
                     LRVFSDDGRLLSDVGRELELYAYLANFRAVRSAVDQVIGQVKSCTPGAEGFVIVPCVT
                     GAEYRVAALEYDGRRIGRLVIGPYRNQEQGHPAASLFALDDSLDRGKVYESFELLPRA
                     KSELGARVAQHLSRVLDLVLFSEHRALLTSNMHLASVRESFRDLEDKNQRLQIAYDRL
                     KELDRLKSNFLATVSHELRTPLTSIIGYSEMLTEGLAGDLTDEQREFIQTIREKGEQL
                     LSLIKGLLDLSKFESGTMTLRKNNTEVAKVVGEVISTMQPSALKKDVRLEQSVESGLP
                     RIWADPERLRQILLNLCENAVKFTPAGGRVLLTAKLTSMDALPMNEVGGMVLLGTRRT
                     GVEFRVADTGIGIPELEREKVFQAFYQVDSSSTREQGGTGLGLSIVKRLVEGHDGQIR
                     IESNEPNGSVFVVTIPVKRATIG"
     gene            3976..5028
                     /gene="thiL"
                     /locus_tag="VFQ61_17355"
     CDS             3976..5028
                     /gene="thiL"
                     /locus_tag="VFQ61_17355"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET9956281.1"
                     /translation="MSRKKSAIGSTTGEFEKIAVIERLHRGAAPRAAWIETGIGDDAA
                     VIEIARLVSVESSLGRSPRRRSSRAGSLDAAGRLVWTVDAQVDGQHFRRDWVDLEDTG
                     YRSFQAAVSDLAAMGAAPVGALSSLALPSALSDVELSALVTGQAIAARECRCPVIGGN
                     LTRAEQLSITTTVLGRARRPLLRSGARPGHELWLIGEVGEAAAGLRALQRGARKTRAV
                     RECIEAWRRPRAQLAAGQRLVGRASAAIDVSDGLMGDAAHLAQASGVRLTISVEQLQS
                     VCSEALRGAARQLGEDELTLALSGGEDYALLATGPRESRPRGARVIGIVERGRGVELD
                     GPRPGLSLHSFDHFRA"
     gene            complement(5072..5506)
                     /locus_tag="VFQ61_17360"
     CDS             complement(5072..5506)
                     /locus_tag="VFQ61_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9956282.1"
                     /translation="MKTKKAQLLASLALSLAVPAALVNLGGCNKKEEPPPPLPSAPPA
                     ATTAPAGPVELVQEVVPPPTASASASTAPVRTGGGSAGSLKACCSALTQNAQSAPEPN
                     KTYMLQAAAVCNAAAGAGQASGAILGSISAALRGAGMPPACK"
     gene            5822..6595
                     /locus_tag="VFQ61_17365"
     CDS             5822..6595
                     /locus_tag="VFQ61_17365"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9956283.1"
                     /translation="MLETAPGMTMFWLLLTGTAFVTSVVSGILGMAGGLILLSALLWK
                     LEPTVAIPVHGVVQLVSNAARSWFLREHVAWRAVLRFILPLLPAGALGLLLLRWVPAD
                     AGRFVIGVFVLLATWAPGAFRLDSLRTNPRLGLPIGGAFVGFFSTLVGATGPLLAPFI
                     LALDLGAQGTIATLAACQIAQHFSKVLLFGLAGFGFATYGLAVALLCGAAIAGTATGT
                     RMLRGISKSRFERVVRWVLTLLAFELLLRGLWRLLPALR"
BASE COUNT         1092 a         2053 c         2233 g         1270 t
ORIGIN      
        1 gcgtccggcg cgaccaccac tgcctccgag agatcgctct tggccaagtg atcgcaaatc
       61 agcggcaccg ccgacagctg atcggcgggg atgtggaaaa agccttgaac ctgcggggag
      121 tgcaagtcca tgctgagcac gcgatccgcc cccgcggttt cgagcaaatc ggccatcaaa
      181 cgcgccgtga tcgaaattct gggctcgtcc ttcttgtccg agcgcgcata ggggaaatac
      241 gggagaacgg cggtgacgcg gcgcgcactc gcgctcttca gcgcgtcgat catgatcagc
      301 agctccagga tcccttccga aaccggcgag cacgaggtct gcacgacgaa cacatcttgc
      361 tcgcgcacgt tctcctcgat ttttactttg aggttttcgt tggagaagcg atgcgtgtgc
      421 gaggcgccga gcgggatccc cgcgtagtgg cagatggcct tcgcaagctc agggtgggcg
      481 gaaccggaaa agatcttcaa ggcgccgttc atggcggcgc agtatagaca cgcgttctgc
      541 acctgcgctg cgtcgcccga acgtttttct gaaggttagc ggagcccctc gtagcgccag
      601 gtctcgtgtg gtgggttgcg atcgaaccag ccagacgtga ccgccaccac gccgagtgcc
      661 aacgcggcgc ccgctcccag cccggcgtac agtgcccagc ttgggattcc tcgtctttcc
      721 gtgggctggg ccgggcctct aaacgcaacc ggctcagttt ggaccccata ccaacgaccg
      781 cattgcgcac gacggcatag cgccacttcc agcaatgggc cgcgcatgcg agcgatggcc
      841 cactgcgggc actcggtcgc tccggcggcg atgggtgcgg gatcgccgtc caaggcaccc
      901 atcagttctt cgcccgagca aggcgccggg gccgagacgg gcagagacaa cacccgctgg
      961 tcgggtgtta cctggatcca gccagcccag accaagcgct cgccgcgcag cacgcggaca
     1021 tgatgcttgc cggggcgggt ggtcaacgcc gagctttgca gcgcgccgtc ccactcgatc
     1081 tcgtcccgcg gccgcagccc ggcgatgccg agactaatac cggtcatggg gggcgggccg
     1141 agttccttgg acttctcctc gcgaaacggc atggcgcgta tgccttccag cgcctcggcg
     1201 tcgtttcgca gctcgcgcgc ccggctccgc agctcggttt gccgcgtgct ggtcggcttg
     1261 aggccggcgc gatccaccac cgccgctcgc tcgaggagct ctgcctcgac gtaatggtgc
     1321 tcggccatga gccaggccgc ttgaggcaac tccgggtgtt cgtcgagctt ccgttcgatg
     1381 tcggcgagcg tgcgctcggc tgcatcggcg tcgaggctgc cgagtgcggt gcgcgccgcg
     1441 tccagggccg cctcgatttg atcggcgaga tccatctctg cggcgttggt gcgcgcggcg
     1501 ggattggggg gctccggtcg actgacgatc tgaaaacgca ccgaccaacg actggagaaa
     1561 gactcgaggt ctcgttgcca gctcgccgac gaaagcaata acgcagccgg accttcggcg
     1621 cgcgcgtacc gtggtgtgcc cgggtcaccc accacctgtg ccggcgtggg ctgtacggtt
     1681 agcagccaga ggggaaggat tgggctcacg gatggctcat gctagtttgc gaaatcggcg
     1741 gggggcgact cacgtcgaat tgcaattgtc cgtttatccc aggtcgaggc attcgtgcag
     1801 ctggacttcg tcaaccgcga gctgaccatc aagctcgtct attacgggcc ggcgctcagc
     1861 ggcaagacta ctaaccttca ggcgctccac aagtgcgccg ccccaggggc gagtggtcgc
     1921 ttgatgacgc tcgagacccg tgatgaccga actcttttct tcgatttgtt gcccctgacg
     1981 ttcaaaagtc agggcagcct ggccgtgcgt ttgaaggtgt tcaccgtacc ggggcaggtg
     2041 atccatgcct cgacccggcg tctggtggtg cagggggcgg acggtatcgc tttcatcgcg
     2101 gattcgcgcg tggtcgagac tcaacacaac gcggactcgt tcctggatct caagcacaac
     2161 ctcaaagagc acgggctcgt gctcaagaag atgccgctca tcatccagtt caacaagcgc
     2221 gatctgccgg acgtccgcaa ggatgacgag atcgcgcagc ttgcggccca ggggcgcgag
     2281 cccgtgtaca tggccgttgc gactcggggc gtgggcgtgg tggagagctt tctcgggctc
     2341 ctgcacttga cctggagtgc gctcgatttc gagcacgagc tcagcaaaaa gtggggcttc
     2401 gacgcgtatg cgttcctcgg tcaggtcgcg gagaagctgg gcgataagcg accgttgcgt
     2461 gagatcttgc gacagaacgt cggcggctcg atcgatgcgc gcccggaagc cgcgacggct
     2521 gccgacaagg ataccaaaca aggagcgggt cgatgacaca gcccgtggcc gcgcgttcct
     2581 tggacgagct gaccctggga gttggggtga aactcgagga cttgctcgac cgcgagggct
     2641 tgtccgaaat gctgcacagc gtttacgact tgttccagct accgctccgc gtcttctccg
     2701 atgacgggcg cctgctctcg gacgtgggtc gagagctcga gctatatgcg tatctcgcca
     2761 actttcgcgc cgtccgtagc gcggtggacc aggtgatcgg tcaggtcaaa tcttgtactc
     2821 ccggcgcgga gggtttcgtg atcgtgccct gcgtgacggg tgcggagtac cgcgtcgccg
     2881 ccctcgaata cgacggcagg cgcatcgggc gtttggtcat agggccctac cgcaatcagg
     2941 agcaagggca cccggcggcc tcgcttttcg cgctcgatga ctcgctcgat cgcggcaagg
     3001 tttacgagag cttcgagctt ttgccccgcg ccaagtccga gctcggcgcc cgagtcgcgc
     3061 agcatttgtc gcgcgtgctg gatttggtgc tgttcagcga gcaccgggcc ttgctcacga
     3121 gcaacatgca cctggccagt gttcgcgaga gctttcgaga cctcgaggac aagaaccagc
     3181 gcctgcaaat tgcctacgat cgtctcaaag agctcgaccg tttgaagagc aactttttgg
     3241 ccacggtctc gcacgaattg cggactccgc tcacgtcgat catcggttac agcgagatgt
     3301 tgaccgaggg gttggcgggc gacctcaccg acgaacaacg cgagttcatt cagaccatcc
     3361 gtgaaaaagg tgagcagctc ttgtcgctga tcaaggggct gctcgatctg tccaagttcg
     3421 agagcggcac gatgacgctg cgtaagaaca acacggaggt cgccaaggtc gtgggggagg
     3481 tgatctccac catgcagcct tcggcgctga agaaggacgt tcggctcgag cagtcggtcg
     3541 agtccggttt gccccggatc tgggctgacc cggagcgtct gcgccagatc ctgctcaatc
     3601 tctgtgagaa tgcggtgaag ttcacgccgg cgggcggccg cgttcttttg accgcgaagc
     3661 tcacgagcat ggacgcgctg cccatgaatg aagtgggcgg catggttctg ctgggcaccc
     3721 gccgcaccgg cgtcgagttt cgggtcgccg ataccggtat tgggatcccc gaactcgagc
     3781 gagagaaggt ttttcaagcc ttttatcagg tcgattcgag ctcgacgcgc gagcagggcg
     3841 gcaccggcct cgggctctcg atcgtcaagc gcctcgtgga gggccatgac ggccagatcc
     3901 gaatcgagag caacgagccg aatggcagcg tgttcgtggt gaccattccc gtgaaacgcg
     3961 ccacaatcgg ctagcttgag ccgaaagaag agcgcaatcg ggtccaccac gggcgagttc
     4021 gaaaagatcg ccgtgatcga gcggctgcat cgcggagctg cgccgcgggc cgcttggatc
     4081 gagaccggca tcggagacga tgccgcagtt atcgagattg ctcgattggt gtccgtcgaa
     4141 tcgagtcttg gacgaagtcc tcgaagacgc tcgagccgcg ctggctccct cgatgccgca
     4201 gggcggctgg tgtggacggt ggatgcgcaa gtcgacggtc agcactttcg ccgcgactgg
     4261 gtggatctgg aggacacggg ctaccgtagc tttcaagctg ccgtaagcga tttggccgca
     4321 atgggagccg cgcccgtagg cgcgctttcg agcctcgcac ttccgagcgc gctgagcgac
     4381 gtagagctct cggcgctcgt cacgggacag gcgattgcgg cgcgggagtg ccgttgcccc
     4441 gtgatcggcg gcaatctgac gcgagcggag cagctgagca tcaccacgac agttctgggt
     4501 cgcgcgcgcc gcccgctcct acgcagcggc gcgcggcccg gacacgagct gtggttgatc
     4561 ggtgaagtgg gcgaggccgc agcgggcttg agagcactgc aacgcggcgc gcggaagact
     4621 cgagctgtgc gcgagtgcat cgaggcctgg agacggccac gtgcgcagct agcggcaggg
     4681 caacgcttgg ttgggcgcgc tagtgcggcc atcgacgtgt cggatggcct gatgggcgac
     4741 gcggcacatc tcgcccaggc aagtggcgtg cgcctgacca tttcggtcga gcaactccag
     4801 tccgtgtgct ccgaggcgct gcgcggagcg gctcggcagc tcggcgagga cgagctcacg
     4861 ctcgcgttgt ccggcggcga agactacgcc ttgctagcca ccggacctcg tgaatcgcgc
     4921 cctcgcggcg cccgcgtcat cggtatcgtc gaacggggac gcggggtcga actcgacggc
     4981 ccgcgtccag gcttatcgct ccactcgttc gatcacttcc gtgcgtgaat acgtccgtgg
     5041 gtgaacacgt ccgtgggaaa acagagcccg ctcacttgca cgcgggcggc ataccggcac
     5101 cgcggagtgc ggctgagatg ctgcccagaa tggccccgga ggcctgccct gcacccgcgg
     5161 ccgcattgca gaccgcggct gcctgcagca tgtacgtctt gttgggttcc ggcgcgctct
     5221 gcgcgttctg cgttagagcc gagcagcagg cttttaggga gcctgccgag ccaccgcccg
     5281 ttcgcacggg tgcggtactc gccgacgccg atgcggttgg cggcggaacg acttcttgca
     5341 cgagttcgac cggcccagcg ggcgccgtgg tagccgccgg gggtgcggag ggcaggggcg
     5401 gcggcggctc ttctttcttg ttgcagccgc ccaagttcac gagcgccgcg ggaaccgcga
     5461 gcgatagggc gagcgatgcg agcaactgag ctttcttggt cttcattggc ctctccaagg
     5521 caaccgaggc gggcagtgcg cccgcgacct ctcgtctttt agccggcatc ctcgaaactt
     5581 ccaaggttgc cgcggcaacc gcggtagcgc cgggaggaag ccatcctgct ggccaggcag
     5641 cctattcctc gccgcagcag gtttgcgggt gaccgcttta tgttcagctc cgacccgctt
     5701 tcgagccgca ccgactcggg ctctggccgc gaggccgtgt cgcgagctac tgcgcctcga
     5761 ttcgtgagac tcgattcgtg agactcgatt cgcgagactc gattcgtgag aagagggggg
     5821 catgctggag accgcgcccg ggatgaccat gttctggttg ctgctgacgg ggaccgcctt
     5881 cgtgacttcg gtcgtctccg ggatcttggg catggccggc ggcctgatcc tgctttcggc
     5941 attgctctgg aagctagagc ccacggttgc gatccccgtg catggagtcg tgcagctggt
     6001 ctccaacgcg gcgcgctctt ggtttctacg cgagcacgtg gcttggcgtg ccgtgctgcg
     6061 tttcatcctg cctctactgc cggcgggtgc attggggctg ttgctactac gttgggttcc
     6121 ggccgacgcg ggacgcttcg taattggcgt gttcgtgctg ctcgcgactt gggctcccgg
     6181 agcctttcgc ttggatagcc tgcgcactaa cccgaggctg ggcttgccta tcggcggggc
     6241 tttcgttggc ttcttttcca ccttggtggg tgcgacgggc ccactgctgg cgccattcat
     6301 tctggctttg gatttgggcg ctcagggcac gattgcaacg ctcgcggcgt gccagattgc
     6361 tcagcacttc tccaaggttc tcctgttcgg cttggcgggt ttcggatttg cgacttatgg
     6421 gctcgcggtc gcgctgctgt gtggtgcggc catcgcgggt accgcgaccg gcacccggat
     6481 gctgcgcgga atatccaagt cgcgtttcga acgagtcgtg cgctgggtgt tgaccttatt
     6541 ggcgttcgaa ctactgctgc gcggactatg gcgcttgctc ccggccctgc gctgagcggg
     6601 cgctcgaggc gggaatgctc cccaggcaga ccctctccaa cgtcgatc
//
LOCUS       DASTKU010000503         7050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1881684, whole genome shotgun sequence.
ACCESSION   DASTKU010000503 DASTKU010000000
VERSION     DASTKU010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881684"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..2757
                     /gene="lon"
                     /locus_tag="VFQ61_17370"
     CDS             211..2757
                     /gene="lon"
                     /locus_tag="VFQ61_17370"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET9956284.1"
                     /translation="MSEIRKLPLLALRGLVAFPGTTVPIAVGRAQSLKAVEEALRGDR
                     RVFAVTEQAETDASGETKLHALGVVAQIEEVQRGIAGQRLLLTPVERATALEYRVEGG
                     VRRALVRPVAEMKPLNADDPAFVGLFREARARAVELGTQAGLSEEALKQVIDAVPEPG
                     PFADLVAAQLELPIAEKQALLETLGVEERLRKLLVHMQRRAEELNAQQRIRNSVNEEI
                     GSKQRELYLREQLRVIQRELGETGDQDKDLQALREKLEALDLPEVARAEVTRGLARLA
                     RVSPESMEAQVTRNHLEWFAELPWNERSEDVLDAVRAERVLDEDHHGLADVKDRVLEF
                     LSVRELAAKRFARAPAHDTSQDGEAKRSPSSHKGSILLFVGPPGVGKTSIAKSIARAL
                     GRKYVRIALGGVHDEADIRGHRRTYVGAMPGRILAGLKQAGTKNPVFLLDEVDKLGAS
                     LHGDPASALLEVLDPAQNSEFTDHYLNVPFDLSEVLFVATANVLHTIPPALLDRMEVV
                     EFSGYTEREKLEIAKRYIVPRKVEEAGLAGHEPPVEFEDAALRSLISRYTRESGVRKL
                     ESQIGAVTRKLARKLAAAQPTELRVDEARVRELLGRPRVHPERAQDFDLAGVSTGMYY
                     TPVGGDIMFVEVATRSLGGAVRSADGPATDFHNLALILTGQLGDVMKESARAALTYAA
                     THAAKLGVPASQLGSIEAHVHVPAGAIPKDGPSAGVTIATALVSSLTGRRVRRDVAMT
                     GEITLKGRVLPIGGVKEKVLGAHRAGIRTIVLPRQNEADLEDIPTEIQRDLEFHFVDT
                     LDQVLAIALLEASPVVVEAAEVPASPPAARSGRELEIEGHPGPWVSKSHE"
     gene            complement(2836..3348)
                     /locus_tag="VFQ61_17375"
     CDS             complement(2836..3348)
                     /locus_tag="VFQ61_17375"
                     /inference="COORDINATES: protein motif:HMM:NF025077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HET9956285.1"
                     /translation="MWKDKSLLLLLATLSMAASQGCVFGSSVALSPEAKHVQIVHESD
                     RPLDCENRGKISGRAHASDEKEAKKNAENEFRNQAADLKANFAYVEAERSGPRGTSSQ
                     REVFIGGQALFCRTEEMKEAEEKRQEEAQKAKEEEEQRAKDEKERAEQEAKEKAEAEK
                     AEKAEKEKKK"
     gene            3752..5755
                     /locus_tag="VFQ61_17380"
     CDS             3752..5755
                     /locus_tag="VFQ61_17380"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HET9956286.1"
                     /translation="MIWLSIWLLPSTAHARDDGPPASERTLAPYFFLEGGDPKLDSFP
                     LQDTRVDVNIAGVLAHVTVTQRYENRGQRPIHAEYVFPASTRAAVHGLSMQIRDQRIV
                     AKIQERKAAERTFEVAKQAKKNASLLTQERPNVFTLKVANIQPKDVIEVELRYSELLV
                     PEAGTYEFVFPTVVGPRYANGAAKPSESQFVASPFLPKGQAAPQALRISGVVSAGLPI
                     RDLSSPSHVLSAQGAPAGASSFQLAPSAEPPNNRDFVLRYRLAGDAIQSGLMLYDGGP
                     GQEKFFALQVEPPQRVPSELVPPREYVFVLDVSGSMQGFPLDTAKQLMRNLVSELRPQ
                     DRFNVLLFSGGSELLSEHSLPGTPANLALAQRFVDTQNGGGGTELLPALERAFALSKN
                     EGLSRSFIVVTDGYIEQDRHAIELVRRRLSDANVFAFGVGTSVNRYLIEGLAKAASGE
                     PFVVLSPAESEKQAKRFYEYVRAPVLTDVHVRFDGFDAYDVEPSAVPDVLAARPVLIQ
                     GKWRGNAKGAIEVTGLSGQGTFRQRFDVQQVVPRSEHQALSYAWARARIASLSDFGFA
                     EPSEAARGQITQLGLRYELLTRYTAFVAVAERVRNASEPAQNVKQPSPLPLGVNNSAL
                     PSETNSADEPELLWMLLIAALVIGGAALQRRQVSPRGQTGEPA"
     gene            5752..6603
                     /gene="xrtK"
                     /locus_tag="VFQ61_17385"
     CDS             5752..6603
                     /gene="xrtK"
                     /locus_tag="VFQ61_17385"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase K"
                     /protein_id="HET9956287.1"
                     /translation="MKRIGAGVLRMCSSRRRDAAFGGAALAITFALKLYYSSASAEGL
                     RWILTPTSWLTKLLTQMQFNWLTDRGYLSNDESVLIAPACAGVNFLIVAFFTLVVLGI
                     GRFESLRTKALFFAGAGAWALFGTILVNALRIAASIHFSPWFTRHLGWSFHSVHRSLG
                     VVLYLSALLLFTGLILGRATVKSPTVDAQSSRDPRCDGERARSSPPRVRFDWVPFAFA
                     LGTYWLFVLVLPGLRWLWAARRNPRALAFDAEAITSHGREVLFISLAVSAVAWMTVRL
                     SHRFKSS"
BASE COUNT         1258 a         2178 c         2191 g         1423 t
ORIGIN      
        1 ccccgactct gtttcggtcg gtgttgcccc gactctgttt cggtcggtgt tgccccgact
       61 ctgtttcggt cggtgttgcc ccgactctgt ttcggtcggt gttgccccga ctgccctcgg
      121 tcgttgcacc cccacgaccg acccgacccg gtcgttcggg tagtgctccg accccttccc
      181 gaacgaaccg atcatgcgca tccaaagtac atgtctgaaa tccgaaagtt gccgcttctg
      241 gccctgcgtg ggctcgttgc tttcccgggg accaccgtcc ctatcgccgt cgggcgcgca
      301 cagtccttga aggccgtgga ggaggcgctt cgaggagatc gacgggtatt tgccgtcact
      361 gaacaggctg aaacagatgc ttcgggcgaa accaagctgc acgcgctcgg cgtggtggcg
      421 caaatcgaag aggttcagcg aggaatcgcc gggcagcggt tgttgctgac tccggtggag
      481 cgcgccaccg ccttggagta ccgcgtcgaa gggggcgtgc gccgggctct ggtccgaccc
      541 gtagcggaaa tgaagccgtt gaatgccgac gatccggcgt tcgtgggttt gtttcgagag
      601 gctcgcgctc gcgcggtaga gcttggaaca caagcgggac tatccgagga agcgctcaag
      661 caagtaatcg atgcggttcc ggaaccgggg ccgttcgccg atctggtggc ggcccagctg
      721 gagcttccga tcgccgagaa gcaagccttg ctcgaaacgt tgggtgtcga ggagcgcttg
      781 cgcaagctgc tcgtccacat gcagcggcgc gccgaggagc tgaacgcaca gcaacgtatc
      841 cgcaactcgg tgaatgaaga gatcggcagc aagcagcgcg aactctactt gcgtgagcag
      901 ctacgcgtga tccagcgcga gctgggggag accggtgacc aagacaagga tctgcaggct
      961 ctgcgcgaga agctcgaagc gctggatcta ccggaggtcg cgcgcgcgga ggtgacgcgc
     1021 gggctcgcgc gcttggcgcg cgtaagcccc gaatccatgg aagcacaagt gacgcgcaat
     1081 cacctggagt ggttcgctga gctaccctgg aacgagcggt ccgaggacgt cctcgatgcg
     1141 gtacgcgccg aacgcgtatt ggatgaggac catcacgggc tggcggacgt gaaagatcgt
     1201 gtcctcgagt tcctgagcgt gcgtgagctc gcggccaaga ggttcgctcg tgcaccggcc
     1261 cacgacacct cgcaagacgg cgaggcgaag cgcagcccca gctcccacaa gggatcgatt
     1321 ctgctgttcg ttgggccgcc aggcgtcggc aagaccagca ttgccaagag catcgcgcga
     1381 gcgctcgggc gcaagtacgt acgaatcgcg ctgggaggtg tgcacgacga agccgatatt
     1441 cgtgggcacc gccgaactta cgtgggcgca atgccgggac gcatcctggc tggtttgaag
     1501 caggccggca ccaagaaccc cgtgttcctg ctggacgagg tcgacaagct gggcgcttct
     1561 ctacacggag atccggcgag cgcgttgctc gaagtgctcg accccgccca aaactccgaa
     1621 tttaccgatc actacctgaa tgtgccgttc gacttgagtg aggtgctgtt cgtggccact
     1681 gcgaacgtac tgcacaccat cccgcctgcg ctcctggatc gcatggaggt ggtcgagttc
     1741 tccggttaca ccgagcgcga gaagctcgag atcgcgaaac gctatatcgt gcctcgcaag
     1801 gtggaagaag cagggcttgc cggccatgaa cccccagtag aattcgagga tgcggcccta
     1861 cgcagcctga tcagtcgcta tacgcgcgag agtggcgtcc gcaagttgga atcccaaatc
     1921 ggagcggtga cgcgcaagct ggcccgcaag ctggcggctg cacaaccgac cgagttgcgg
     1981 gtggatgagg cccgggtccg cgagttgctc ggacgaccgc gggtgcaccc cgagcgcgcg
     2041 caggacttcg acctcgccgg tgtttcgacg ggcatgtact acacaccggt aggcggagat
     2101 atcatgttcg tcgaagtggc gacccgcagc ctgggtggtg cagtacggtc cgcggacggt
     2161 ccagccaccg atttccacaa tctggctttg atactaacgg gccagttggg agatgtcatg
     2221 aaggagagcg cgcgtgccgc gctgacctat gccgcgaccc acgccgcgaa actcggggtg
     2281 cccgcatcac aactcggcag catcgaagct cacgtccatg tcccggcggg cgcaattccc
     2341 aaagatggac cgagcgcggg tgtcacgatt gctaccgctc tggtgtcgtc tctcacgggg
     2401 cggcgggtac gtcgagacgt ggccatgacg ggcgaaatca cgctcaaggg gcgcgtgctc
     2461 ccgattgggg gcgtcaagga gaaggtgctc ggcgcccacc gcgcgggaat tcgcacgatc
     2521 gtgctgcccc gtcagaatga agccgatttg gaggacatcc cgactgaaat acagcgcgat
     2581 ctcgagttcc acttcgtgga taccctcgac caagtgctag caatcgcgct gctggaggcg
     2641 agcccagtcg tggtggaagc ggccgaagtt ccagcctcgc cgcccgcggc acgcagcggg
     2701 cgagaactgg agatcgaagg ccatcctggg ccttgggtat ctaagtctca cgaataaccc
     2761 gtagcagctc acgccgcttt cccgccgtgc ggccattaag ggcccggatc ggataaccgc
     2821 tccgggcctt tgctttcact tcttcttctc tttttcggct ttctcggcct tctctgcttc
     2881 ggctttttcc tttgcctcct gctcggcgcg ctccttctcg tcctttgcgc gctgctcttc
     2941 ctcttctttg gctttttgcg cttcttcttg tcgcttttcc tcggcttcct tcatctcctc
     3001 ggtgcgacag aacagcgctt ggcccccgat gaagacctct cgttgcgaac tcgtgccgcg
     3061 tggcccagag cgctcggctt ccacgtacgc aaagtttgcc ttcaaatccg cggcttggtt
     3121 acggaactcg ttctcagcgt tcttcttggc ttccttctcg tcactcgcat gggcacggcc
     3181 gctgatcttc cctcgatttt cgcagtcgag aggccgatca ctctcgtgca cgatctgtac
     3241 gtgcttggcc tcgggactca aggccacgct gctgccgaag acgcagccct gggaagcagc
     3301 catggatagg gttgccagta gcaggagtag cgacttgtct ttccacatag gggcaatctc
     3361 tcctcgcggc gcaggttagc ctagaagtgc cggtttcaca cccctcgctc tgctactcgg
     3421 gccaatcttg cgggctcggg tcgggtgaag gtcgaagcgt ggggtaccac ttcacatgct
     3481 gcggtgggcg tgtgcccggg agcgcgccgc tgtgggccgc gagacacgcg gagatttgcc
     3541 gtggctcgcg gcacgcagca attttccagc gtttggtggg gcttccggag agtcatgagg
     3601 gtgagcacac cggttgcaat ccgctagttg cgtgatgcac aaaaggcttg cccagtctca
     3661 gaacgaaccg gcgtcccgcg cgggttcccg actactccag gctgaaactc gcttcgaaga
     3721 actccgcggc taccgcgttg ttcgagcagc attgatctgg ttatcgatct ggctgctgcc
     3781 gagcacggcg cacgcgcgcg atgacggtcc ccctgcttcc gagcgcacgc ttgctcccta
     3841 tttcttcctc gaaggtggag atccgaagct ggattccttt ccattgcagg acacgcgagt
     3901 cgacgtaaac atcgcgggcg tactcgccca cgtgacggtc acgcagcgct acgagaaccg
     3961 cgggcaacga ccgattcacg cagagtacgt gttcccggcg tccacgcgcg cggccgtcca
     4021 tgggctcagt atgcagatcc gcgaccagcg catcgtggcc aagattcaag agcgaaaagc
     4081 ggccgagcgc accttcgagg tcgccaaaca ggcgaaaaag aacgccagct tactcacgca
     4141 ggagcggccc aacgtgttta cgttgaaggt cgcgaacatc caacccaaag acgtgatcga
     4201 ggtcgagctc cgatatagcg agctcctagt tcccgaggcg gggacctacg agttcgtgtt
     4261 tcctacggtc gtgggtccac gctacgcgaa cggcgcggcc aagccgtcgg agagccagtt
     4321 cgtcgccagt ccgttcttgc ccaagggtca ggccgccccg caggctctac gcatttcggg
     4381 cgtcgtgtct gcgggcttgc cgattcgaga cctctcgagt ccgtcacacg tgctcagcgc
     4441 gcagggcgcg cccgcaggcg cttcgagctt tcaattggca ccgagcgccg agccaccaaa
     4501 caatcgcgac ttcgtgctgc gttatcggct cgccggagat gccattcagt ccggattgat
     4561 gctctacgac ggcggacctg gtcaggaaaa gttctttgcc ttgcaggtcg agccgccgca
     4621 gcgggtgcca tccgagctag tgccgccgcg tgagtacgta ttcgtactcg atgtgtccgg
     4681 cagcatgcag ggttttccgc tcgacacggc caagcagctc atgcggaatc tcgtctccga
     4741 gctccggccc caagatcgct tcaacgttct attgttctcg ggcgggtcgg aactgctgtc
     4801 ggaacactct ctaccgggca cgccagcaaa tctggctttg gcccagcgct tcgtggacac
     4861 ccaaaacgga ggtggtggta ccgagttgct ccccgccttg gagcgcgcat ttgcgctctc
     4921 caaaaacgaa gggttgtctc gcagcttcat cgtcgtgacg gatggctata tcgagcaaga
     4981 ccggcacgcc atcgaactgg tccggcgcag attgtcggat gccaacgtat tcgcgttcgg
     5041 ggtgggtact tccgtcaatc gatatctgat cgagggccta gccaaagctg catccgggga
     5101 accgttcgtc gtgctgagcc cagcagagtc ggagaagcaa gccaaacgat tctacgaata
     5161 cgtgcgagcc ccggtgctga ccgacgtgca cgtgcggttc gatggcttcg atgcgtacga
     5221 cgtcgagccc tccgcggtgc ccgacgtgct cgcagcacgg ccggtcttga tccagggcaa
     5281 gtggcgcggc aacgcgaaag gagccatcga ggtgacgggg ctttccggcc aaggtacgtt
     5341 tcgccagcgc ttcgacgtcc aacaggtcgt tccccgcagc gaacaccaag cgctctctta
     5401 cgcctgggct cgagcccgca ttgcgagcct gtccgatttc ggttttgcag agccgagtga
     5461 agccgcgcgc gggcaaatca ctcaactcgg gttgcgctat gagttgctga ctcgatacac
     5521 agcgttcgtt gccgtcgccg agcgcgtccg caacgcctcc gaacccgctc agaacgtgaa
     5581 gcaaccctcg cccttaccgc tcggggtcaa caactcggct ctaccctctg aaaccaactc
     5641 cgctgatgaa cccgagctgc tgtggatgct gctgatcgcg gcgctggtca ttggaggggc
     5701 tgctcttcag cgccgacagg tcagtccgcg ggggcaaacc ggagaaccag cgtgaagagg
     5761 atcggtgccg gagtgctccg catgtgctcg agccggcgtc gcgacgcggc gttcggcgga
     5821 gccgctttgg ccatcacctt cgcactgaag ctctattaca gctcggcgag cgcggagggg
     5881 ctacgttgga ttctgacgcc gaccagctgg ctaacgaagc ttctgaccca gatgcagttc
     5941 aactggttga ctgatcgagg ttacctttcg aacgacgagt ctgtgctcat tgccccagcg
     6001 tgtgccgggg tcaattttct aatcgtcgcc ttctttacgc tggtcgtttt aggcatcggg
     6061 cggttcgagt cacttcggac caaggccctt ttctttgcgg gtgccggggc atgggcgctg
     6121 ttcggcacaa tcctggtgaa cgcgttgcgc atcgcggcgt cgattcactt cagcccttgg
     6181 ttcactcgcc acttgggctg gagctttcac tcggttcacc ggtcgctcgg cgtcgtcctt
     6241 tacctatctg cactgctact cttcacgggg ctcattctcg gacgggccac cgtgaagagc
     6301 cccacggttg acgcccagtc gagtcgcgat ccgcggtgtg atggtgagcg tgcccgttct
     6361 tcgccgccta gggtacgctt tgactgggta ccatttgcgt tcgcgctcgg aacctattgg
     6421 ttatttgttc tagtgctgcc cggtttacgc tggctatggg cggcacgtcg caatcctcgg
     6481 gccctagcct tcgacgccga ggcaatcaca tcccacgggc gcgaagtcct ctttatctcg
     6541 ctcgccgtct cggcggttgc atggatgact gttcggcttt cccaccgatt caaatcctcc
     6601 tgagtggcct gcatttccgt ctcgaggtct tgcgggttcg gcctccgttc gaacaggcca
     6661 gaaacccggt tcatccacga aacgacaaat ttcaaaaaag aggggctcgc ggtgatcaca
     6721 tgccgcatat gcgggcatga ggacgcggta gccagtagtg tgcgccgcga gtacgagtaa
     6781 caacctccaa aatgaatcat gaacaaggct ggttgagaat ccactcgagt tgccgaccgc
     6841 gcgcgaaatg aacgcaggga aactcggatc gcatcaactc gcggccgctc gtttgaacca
     6901 cgagctcagc cctgcggcgt cgacacttca atgctgctcg tgacttctcg tacggagcgc
     6961 gcgacttcgt gggcgcttct ggacgtcgcc acgtttcgat ccgtcagttg gctgccgttg
     7021 gccctttcgg gcgtgggggg gcaaatgagc
//
LOCUS       DASTKU010000504         4264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_96549,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000504 DASTKU010000000
VERSION     DASTKU010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96549"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFQ61_17390"
     CDS             complement(<1..714)
                     /locus_tag="VFQ61_17390"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9956288.1"
                     /translation="MAHRASLNTLRLASLFAGAASVAIVVQQALMEYAPPVRCAAGLV
                     AQAGRCCGGGQTWFNGRCEGHVTSCGPNQFALEVDGEGRDCVFDVPPVELPGGHLPQG
                     AADFQLEGVTGSLDIRPFAIDRGEVTVYRYNECIRREACSGPPPDREPGLPVTGISPQ
                     AAEAFCAFAGGRLPEAAEWRFAASGLQGRRFPWGFTGLVCRRASFGLLHGPCSDVGAG
                     PDLVGARPDGNTPEGIADLS"
     gene            complement(716..2776)
                     /gene="ligA"
                     /locus_tag="VFQ61_17395"
     CDS             complement(716..2776)
                     /gene="ligA"
                     /locus_tag="VFQ61_17395"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET9956289.1"
                     /translation="MSLAPAKAKARHDQLAKELKAHDFRYYVLDDPAISDLEYDQLYG
                     ELKALEAEYPALVTPDSPTQRVAGAPRGELRNVAHAVPMMSLDNTYSEEELRDFVRRV
                     LDGLPTGRTPEFCVEPKLDGASIEVLYRDGRMVEGSTRGDGITGEEITVNLRTIRSLP
                     LHIEYKGPLTLRAEVVIYKKDLERINRVRTEAGEAPFANPRNAAAGSLRMIDPRVVAE
                     RPLRALVWQVIEGAELAGSHSAALDKLEELGLPTHRKHVVCKTVDEVVAAIHELDSAR
                     KTYPYETDGAVVKVNEFGNQAILGATAKFPRWAIAYKFSAEQATTRLLGIQVGVGRTG
                     TLTPIAELEPVHLSGTVVSRASLHNEQIVGQLDVRIGDYVSIAKAGEIIPQVISVDLS
                     RRDGTEQAWRMPDHCPTCGTAVVRSTTEVAIKCPNVRCPDQVKGALFHYSRRFAMDVD
                     RLGESLISELVQKELVRDVADLYDLTLEQVLSLTRMAQKSAQNVIESIERSKQRGFDR
                     LLTGLGIEHVGQVAAKQLAEAAHSLPQLLSWTPEQTREHIAAVAGFGPKMVESVVAFV
                     HGESSRKLLERLKERGVSVPMERKASAVEGPLVGQIFCVTGVLSRKREDVHQAIRDAG
                     GEIHDKVKKGTTFLVAGDKVGGTKLTAAKKNGTRVIDEATLDKILVEGLAALPAEPSA
                     EEAS"
     gene            3076..3879
                     /locus_tag="VFQ61_17400"
     CDS             3076..3879
                     /locus_tag="VFQ61_17400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET9956290.1"
                     /translation="MKPEAERFLREGSGPLDDLALTLPVFVGYHLGVVFLPMRNAADM
                     VTRELTNLAKNDLGVYSLITITLGFAFAGILLILGRRQSLRAWRFLLVGLEGILYAVA
                     MRVVAGYIVGRLHLGFDLLSHLGFIPDPSTFRDAAAAGAAASEPGVIQPLTNSAFAGA
                     IMSLGAGFYEELVFRVVLFGLGFRLFSHGFPATAAAKRGLFAVMWAVLCALAFSGWHY
                     VGEFGDPFEVRSFVFRWVCGLVFTLIYAFRGFAPAVWTHALYDLWIMVF"
     gene            4039..>4264
                     /locus_tag="VFQ61_17405"
     CDS             4039..>4264
                     /locus_tag="VFQ61_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956291.1"
                     /translation="MPITIQLEHVALIAGTLSAHASNLGSDIKATLHGNHLRLWQGQA
                     PVFEIRVDIQGKNVTLNRAETPVLERGRGWG"
BASE COUNT          718 a         1552 c         1218 g          776 t
ORIGIN      
        1 cgataggtcc gcgatgcctt ccggcgtgtt gccgtcgggc cgcgctccca ccaagtcagg
       61 gccggcgccg acgtcactac aggggccgtg aagtaacccg aagctcgcgc gccgacacac
      121 gaggccggtg aagccccagg gaaagcgccg cccttgcaaa cccgaagcgg caaagcgcca
      181 ctccgcagct tcgggtaagc gcccgcccgc gaaggcgcaa aaagcctccg ccgcttgcgg
      241 cgagatccca gtgactggca aacctggctc ccgatccggg ggaggccccg agcaggcctc
      301 gcgccgaata cactcgttgt atcgatatac ggtaacttca ccgcgatcga tcgcgaatgg
      361 tcggatatcc agactcccag tcactccttc gagttggaaa tcggccgctc cttgcggcag
      421 gtgcccaccc gggagttcta cggggggaac atcgaacacg caatcacgcc cttcgccgtc
      481 aacctccaag gcaaactggt tgggcccgca actcgtgacg tgcccctcac agcgaccgtt
      541 gaaccaagtt tgcccgccgc cacagcaccg cccggcttgc gccacgagcc ccgctgcaca
      601 gcgtaccgga ggggcgtact ccatcagcgc ttgctgcacc acgatcgcca ccgaggccgc
      661 gcccgcgaag agcgaggcca agcgcagcgt gttgagcgaa gcgcgatgag ccacctcagc
      721 tggcctcttc ggcgcttggc tcggcgggta acgcggccaa gccttcgacc aagatcttgt
      781 cgagcgttgc ctcgtcgatc acgcgcgtgc cgttcttctt ggccgccgtc agcttggtgc
      841 cgcccacctt gtcgcctgcc accaagaacg tggtgccctt cttgacctta tcgtggatct
      901 caccgcccgc gtcgcggatg gcttggtgta cgtcctcgcg tttacgtgaa aggacgcccg
      961 tcacacagaa gatctggccg acgagcggcc cttccaccgc gctcgccttg cgctccatcg
     1021 gcacggagac gccgcgttcc ttgagccgtt cgagcaactt acgcgagctc tccccgtgca
     1081 cgaacgccac cacgctctcc accatctttg gcccaaaccc ggccacggcc gcgatgtgct
     1141 cccgcgtttg ctccggcgtc cacgagagca gctgcggcag actgtgcgcg gcctcggcca
     1201 actgcttcgc cgcaacctgg cccacgtgct cgatgccgag gccagtgagc agccgatcga
     1261 agccgcgctg cttgctgcgc tcgatcgact cgatcacatt ttgcgcgctc ttttgcgcca
     1321 tgcgcgtcag gctcaaaacc tgctccagcg tcaaatcata gaggtcggcc acgtccctca
     1381 cgagctcctt ttgcacgagc tccgaaatca gggactcacc taagcgatcg acgtccatcg
     1441 cgaagcgccg tgaatagtgg aacagcgcgc ctttgacttg gtccgggcag cgcacgttgg
     1501 ggcacttgat ggccacctcg gtcgtgctcc gcaccacggc cgtgccacag gttgggcagt
     1561 gatccggcat gcgccacgct tgctcggtgc cgtcgcgacg cgacaaatcc accgaaatga
     1621 cctgaggaat gatctccccg gccttggcga tactcacgta gtccccaatc cgcacgtcga
     1681 gttgccccac gatttgctca ttgtgcaatg acgcgcgcga caccaccgtg cccgacaagt
     1741 gcaccggctc gagctccgcg atcggcgtca acgtcccggt gcgccccacg ccgacctgaa
     1801 ttcccagcaa ccgagtggta gcttgttcgg cgctgaactt ataggcaatc gcccagcgcg
     1861 ggaacttcgc cgttgcgccg aggatcgcct gattgccgaa ctcgttcact ttgacgaccg
     1921 cgccatccgt ctcgtacgga tacgtcttgc gcgccgagtc gagctcgtga atagcggcca
     1981 ccacctcgtc caccgtcttg cagaccacgt gtttgcggtg tgtgggcagc cccaactcct
     2041 cgagcttgtc gagcgccgcc gagtgcgaac cggcaagctc tgccccttcg atcacctgcc
     2101 aaaccaaagc ccgcagtggc cgctcagcca ccactcgcgg atcgatcata cgtaagctcc
     2161 cggccgcggc gttgcgcgga ttcgcgaacg gggcctctcc cgcctccgtg cgtacgcgat
     2221 tgatgcgctc caggtccttc ttgtaaatca ccacctccgc ccgcagcgtg agcggcccct
     2281 tgtattcgat atgcagcggc aacgagcgaa tcgtgcgtag attcaccgtg atttcttcgc
     2341 cagtgatgcc gtccccgcgc gtcgaaccct ccaccatgcg cccgtcccga tacagcacct
     2401 cgatgctggc gccgtccagc tttggctcca cgcagaactc cggcgtccgc cccgtcggca
     2461 gcccgtcgag cacgcgccgc acgaaatccc gcagctcctc ctcggagtac gtattgtcga
     2521 gcgacatcat gggcaccgcg tgcgccacat tccgcagctc gccgcggggc gcccccgcca
     2581 cccgctgagt gggcgagtcc ggcgtcacca aagccggata ctcggcctca agcgccttca
     2641 gctctccgta aagctgatcg tattcgaggt ccgaaatcgc cggatcgtcc agcacgtagt
     2701 accggaaatc atgggccttc agctccttcg cgagctggtc gtgccgggct ttcgccttgg
     2761 ccggtgcgag gctcatgcgc accttccgtt ccagcaaccc aagccaccgc ccttgcggca
     2821 aacccaaccg agcaaggaac gagacgacgt agagagcaaa acaggcacca cggccggcag
     2881 catagtcatt tccggagagc ggatctggca cagatgcggc gttcgtcgag gcgcggcccg
     2941 tcgaccctcc gcccgcagca aaaccagccg ttttcttgag tttccaggct cgcggaatac
     3001 tctggcgccg aagcccgtct gtcatgacat ccaccgggat ccccaccgca gcgagatgaa
     3061 agccaggtca gcgctatgaa acccgaagcc gagcgctttc tgcgcgaggg cagcggcccg
     3121 ctcgacgacc tggccctcac cctgcccgtc ttcgtcggct atcacctcgg tgttgtcttc
     3181 ctcccgatgc gcaacgccgc ggacatggtc acccgcgagc tgaccaacct ggccaagaac
     3241 gacctcggcg tctattcact catcaccatc accctgggct tcgccttcgc cggcatcctt
     3301 ctcatcttgg ggcggcgcca aagcctgcgc gcgtggcgct tcctgctcgt gggtctcgaa
     3361 ggcatcttgt acgcggtcgc gatgcgcgtg gtcgccggct acatcgtcgg tcgcctccat
     3421 ctcggcttcg acctgctctc ccatctcggc ttcatccccg acccgtccac cttccgagac
     3481 gcggccgcgg ccggcgcagc ggcgtccgaa cccggcgtga tccagccgct cacgaactcc
     3541 gcgttcgccg gcgcaatcat gtcgctcggg gccgggttct atgaagaact ggtgtttcgc
     3601 gtggtcctat tcgggctcgg cttccggctc ttctcccacg gcttccccgc caccgcggcc
     3661 gccaagcgcg gcctctttgc cgtaatgtgg gccgtactgt gcgcgcttgc attcagtggg
     3721 tggcactacg tcggagagtt cggcgacccc ttcgaggtcc gctcgttcgt gttccgctgg
     3781 gtctgcggcc tcgtcttcac gttgatttac gccttccgcg gttttgctcc cgccgtgtgg
     3841 acccacgcgc tgtacgacct gtggatcatg gtcttctagc cctgcgggtt tgggccacag
     3901 aacttcgggc gcgccgcccg tccctcatcc tcgaagcggc cacccgtccc ccctttgcgc
     3961 cccccgcagc cgcgcctgcc cccgcgataa ccgcgaattg gagcgtccac gtcgagtagc
     4021 gtgcgttagc atccctgcat gcccatcacg atccaactcg aacacgtcgc gctcatcgcg
     4081 ggcaccctga gcgcccacgc ttcgaacctc ggttccgaca taaaagccac tttgcatggc
     4141 aaccacctga ggctctggca ggggcaagcg cccgtgttcg aaatcagagt ggatatccaa
     4201 ggcaagaacg tcaccttgaa tcgcgccgag acgcccgtgc tcgagcgcgg ccgcggctgg
     4261 ggca
//
LOCUS       DASTKU010000505         8883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1211727, whole genome shotgun sequence.
ACCESSION   DASTKU010000505 DASTKU010000000
VERSION     DASTKU010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8883
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211727"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFQ61_17410"
     CDS             <1..571
                     /locus_tag="VFQ61_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956292.1"
                     /translation="REAQGGGSGIDVAASTYGGILLVRRQSDSLHCEPVRLPAGLRIH
                     VLSEPRPASTQNFVARVRALANDRPAEHRACLQRQAEASERAARALLDGDADALLAAL
                     AAQGEALLALGLAANIPIFTEALAHLRPEAIRAGAALLPSGAGGGDLALWVTRERAGE
                     SPTTLPPETPELPRLMLHLGAPGVARVPD"
     gene            585..1895
                     /locus_tag="VFQ61_17415"
     CDS             585..1895
                     /locus_tag="VFQ61_17415"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET9956293.1"
                     /translation="MSTPGSRIPGFYRLPLEERRGLIAERAQIELNELVRAVEGGGLD
                     VAVADKVVENVLGTYGLPFGIALNVRVNGRDRLVPMVVEEPSVVAAASNAARMVRASG
                     GFSAEVAESLMIGQVQLSGVPHPHAAVARLEAAQSQLLEIAAGAVPGLVNRGGGPKKI
                     EVRDLGQGNIVLHILVDCRDAMGANLVNSIAEAVGPTARELSGGVLGLRILSNLCDRR
                     RVRATCRVHGNELLGSRADEDSDEDGRRVIDAIVAASRFAELDPYRAATHNKGIMNGV
                     DAVVIATGNDFRAVEAGAHAYAARSGRYAPLAVWRRDGEDLYGELEMPLSLGIVGGTL
                     RVHPTAQLALKILDVQTADELSTIAASVGLASNLAALRALATEGIQRGHMSLHARAVA
                     TSAGASGAEVERVAEIIAARGQISRETAEAVLVQLRTGSIVPVL"
     gene            complement(1969..3030)
                     /locus_tag="VFQ61_17420"
     CDS             complement(1969..3030)
                     /locus_tag="VFQ61_17420"
                     /inference="COORDINATES: protein motif:HMM:NF019896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HET9956294.1"
                     /translation="MPLTETTWLVSGSLPGALPYRLAVEPLRAMMTGYLEPSRTRIPE
                     WQAFYRGSYGPGSNGPNRRAWLPQLCWGESCNAAVNPETTAEFTPPEFAAPSMAPADW
                     RLVGGSFVGLELADPTALRQDTGLGAGATPTLPWLLLGSEVLNSTPEMLAVVRGRPCP
                     SARTPRPVRIVRYAGETERLELTDCDGAIASDALERLSVLARPPNTARPELPLPLHPQ
                     GELGEWMPELRLLHPRLAWLVSRIAEAFPQRSIVLMSGYRRDAHSGLHQKGQALDLYV
                     QGVPNEDLFRFCRTLSDAGCGFYPNHPFVHVDVRSFGKGHVTWVDVSDPGEQSVYVDG
                     WPGVLAPGIGWLGPRVATN"
     gene            complement(3571..4215)
                     /locus_tag="VFQ61_17425"
     CDS             complement(3571..4215)
                     /locus_tag="VFQ61_17425"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9956295.1"
                     /translation="MMNTETVASARKPTEENGSARSADWDNPRVASILSAAAKCFARK
                     GFTATTLAEIGKELGLRKSIVHYYFASKSALIHEVQSFTYHRYLDRLKEAIRNGDGSP
                     QQAADALRALWDAIQNNKTGTGLNIEVWSAARRDAELKRRASGLQRDARQVIRDAIAP
                     VTQKAGPAMADSLATLILAVLNGLSVTEYLEGEEAKVKEAYDTFLELLRTGFDS"
     gene            complement(4320..5945)
                     /locus_tag="VFQ61_17430"
     CDS             complement(4320..5945)
                     /locus_tag="VFQ61_17430"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HET9956296.1"
                     /translation="MPSRFSPSRRTFAALAVAILAGIVSGFFLLPRSAGPEDALANRA
                     TPLFLGQSLPQPNLPDAQRAALARARSYLAGRFRLVLPDKSEREIYLGQFGVELDKVH
                     LARLVRDSRDRTSALYRNLPAVEVMSQVALPLPIAIDRSTALARLFALKDELDRLPAD
                     ARLDLEKRELIKEVYGRKLDVDASLEAIERALRAGKDRVPLVFLEQKPRRLASELGNV
                     EFSEVVGYFETNYDRSQRQEARTYNLRLAASRLDGHVLLPGEIFDFNAVVGPRDEANG
                     YKVAPVIAEGELVDGIGGGTCQISGTLHGAAFFGGLEIVERYPHTRPSSYIKMGLDAT
                     VVYPTINFRIRNPFPFPVVLHETVKNGVVRAEILGPKKPLTVTLIRRVLDSIPYEEVE
                     RPDKSLPSGTRVLGQRGVPGFKLRRYRIVRDGLHAVRERWDDVYPPTTQIVRVGTGEK
                     DADSDSKKAVDDPHPEYLADELLVMTLGDSDEGKPSSFAENREPGRFGQAGWTEEAGM
                     PLYKSREQTAERPGRTSETGKKSRKKSRSDERT"
     gene            6096..8243
                     /gene="uvrC"
                     /locus_tag="VFQ61_17435"
     CDS             6096..8243
                     /gene="uvrC"
                     /locus_tag="VFQ61_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET9956297.1"
                     /translation="MIDLRRNKVTDPTPPPQTPSPPSAVDPLELKLASLPTQPGCYVF
                     RDRAGAVLYVGKAKSLRARVRSYFQAQSGDTRAFIPFLREEVGDFETIVAATEKEATI
                     LENHLIKERKPRYNVKLRDDKEYLSLRISSEHEWPRLELVRRPERDGARYFGPYHSAT
                     AARRTLNVVEKHFKLRTCTDRELSSRKRPCLEYQIKRCPGPCVYEVDRAAYAAQVRAV
                     ALFLDGRHDELVRELEERMREASSRMEFELAAAYRDQLQAVQQVRQAQRVAAVTDRDQ
                     DVLGIYREGDLVELTLMVVRGGRVIDVGSFSNRRVEVPDDEVMASFLREHYGEGGGGE
                     ALIPDEILLPALPEGVDGVREWLDERRQALAKERGERAPRRIEIMLPMRGPRRALLDL
                     ALQNAEHAFKEKRRTADDIEERLLKVQARLRLPSVPHRIECVDISHHGGSDTVGAVVA
                     LADGTPDKKRYRTYHVRGVAEGDDYGAIYQVLSRRFRRGLKAQDTSSEAEDETAWDLP
                     DLFVVDGGRGQLGVALAAAHDLGLHTLPIVGLAKERESLTGDKQVDRVYLPGQKNPIP
                     LRPNSPELFMLARARDEAHRFSNRGRKKAGRRRTWSSRLEDVPGIGPKTRKALLRHFP
                     SVQAIFEATEEQLREVPTLTRKHITALLAARAEPPNISGEPDLDGGDTELEDAELGDL
                     DVEEETPAEDADGVEPPEPEPLSNARDVLGTGN"
     gene            8563..>8883
                     /locus_tag="VFQ61_17440"
     CDS             8563..>8883
                     /locus_tag="VFQ61_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956298.1"
                     /translation="MMLKSQAPTKPNGVLRWLRVAAVGVAAAAGATGLTVGCLDRPVN
                     PQDPKTSNVYVAEIRQTAVDKIDLLFMIDNSASMADKQEILGDAVPVLLSRLITPVCV
                     DEKTG"
BASE COUNT         1584 a         2864 c         2857 g         1578 t
ORIGIN      
        1 tcgcgaggca cagggcggag gaagcgggat cgatgtcgcg gccagcacgt atggtgggat
       61 cctgctcgtg cgccggcaat cagactcgct ccattgcgag cccgtgaggc taccggcggg
      121 tctccgcatc cacgtcctct cggagccgag gcctgcttcc actcagaact tcgtcgcacg
      181 agttcgtgcc ttggcgaacg accgtcccgc ggagcaccgc gcgtgcctac agcgccaagc
      241 cgaggcttcg gagcgggccg cgcgcgcatt gctggacggc gatgcggacg cgcttttggc
      301 ggcgctagcc gcgcaaggtg aggcgctatt agccctcggt ttggccgcca acattcctat
      361 tttcactgag gcgctggccc acctgcggcc cgaggcgatc cgcgcggggg ctgcactgct
      421 gccgtcgggg gccggtggtg gggacttggc gctgtgggtg acccgcgagc gggcaggaga
      481 atcaccgacc acgttacccc cagagacacc cgagctgcct cggctcatgc tccaccttgg
      541 agccccaggc gtggctcgcg ttcctgatta ggaaggtcct cgatatgtct actcccggat
      601 ctcgaatacc aggtttctat aggcttccgc tcgaagagcg ccggggcttg atcgcggagc
      661 gagcgcaaat cgagctgaac gagctcgttc gtgccgtgga aggtggtggc ctcgacgtgg
      721 cggtcgcgga caaggttgtc gagaacgtgc tcggcactta cggactgccc ttcggtatcg
      781 cgctgaacgt gcgcgtcaat ggccgcgatc gtctggtccc gatggtagtg gaagagccca
      841 gcgtggtggc cgccgcctcg aacgccgcgc gcatggtccg cgccagcggt gggttctccg
      901 ccgaagtcgc ggagtccctg atgattgggc aagtgcagtt gagcggagtg cctcacccgc
      961 acgcggcggt ggctcgcctc gaggcggccc aaagccagct actcgagatc gcggcgggcg
     1021 ccgtgcccgg cttggtcaat cgcgggggcg ggccgaagaa gatcgaggtg cgcgacttgg
     1081 gtcaaggcaa catcgtgttg cacatcttgg tggattgccg agatgcgatg ggcgcaaacc
     1141 ttgtcaacag catcgcggag gccgtgggac ccacggcacg tgagctgagc ggtggcgtgc
     1201 ttgggttgcg gattctttcg aacctttgcg atcgccgacg ggtgcgtgcg acgtgccggg
     1261 tgcatgggaa cgagctgctc ggttcgcgcg cggacgagga ttccgacgaa gacggccgac
     1321 gcgtcataga cgcgattgtc gcggcttccc gctttgcgga gctggatccc taccgtgcgg
     1381 ccacacacaa caaaggcatc atgaacggcg tggacgcggt agtgattgcg accggcaacg
     1441 acttccgcgc cgtggaagcg ggcgctcacg cctatgccgc gcgctcgggc cgctatgctc
     1501 cgctcgcggt ctggcggcgt gacggagaag atctgtatgg cgagctcgag atgcccctct
     1561 cgctgggtat cgtcggtggt accttgcgcg ttcacccgac ggctcagctc gcactgaaga
     1621 ttttggacgt gcagacggcg gatgagctga gcaccattgc cgcctccgtc gggctcgctt
     1681 ccaatctggc ggccttgcgc gccctcgcca ccgagggcat tcagcgcggc cacatgtcgc
     1741 tgcacgcccg cgcggtggcc acctcggcgg gtgcttcggg cgccgaggtc gagcgcgtcg
     1801 ctgagatcat cgcggcccgc ggccagatta gccgtgaaac tgccgaggcg gtgttggtcc
     1861 aactgcgcac cggcagcatc gtccccgtgc tctgacaacg ctcgcccgaa cctagttgtc
     1921 cgaacctgtc gtccgagctt actcgctgaa ccgcgggcgc ggatcgactc agttcgttgc
     1981 gacgcgaggc ccgagccagc cgatgcccgg ggccagcacg cccggccacc cgtcgacgta
     2041 tacggactgc tcgccggggt cgctcacgtc gacccaagtc acgtgaccct tgccgaaact
     2101 gcggacgtcc acgtggacga acgggtggtt gggataaaac ccacaacccg catcactcag
     2161 cgttcggcaa aaacgaaaga ggtcctcgtt cggcacgcct tgcacgtaaa gatccaaggc
     2221 ctgacccttc tgatgcaggc cactgtgtgc gtcgcgccga tagccgctca tcaacacgat
     2281 cgagcgctgc gggaaggctt cggcgatgcg cgagacgagc caggcgaggc gaggatgaag
     2341 taaccggagt tccggcatcc actctccgag ctcaccctgc ggatggagtg gcaacggcag
     2401 ctccgggcgc gcggtgtttg gtggtcgagc caatacactc agccgctcga gcgcgtccga
     2461 cgcgatggcg ccatcgcagt cggtcaactc gagccgctcg gtttcacccg catagcgcac
     2521 gatccgaacc ggtcgtggcg tgcgcgcgct cgggcaaggg cgcccacgca ccaccgccaa
     2581 catctccggt gtcgaattca ggacctcact acccagcaag agccagggca gggttggtgt
     2641 tgcacctgct ccaagtcctg tgtcctgccg cagcgcggtc ggatccgcga gctcgaggcc
     2701 gacgaacgag ccgcccacca gacgccagtc tgcgggagcc atgctgggtg ccgcgaactc
     2761 cggtggcgtg aactctgcgg tcgtttccgg gttcaccgcc gcgttgcagc tctctcccca
     2821 acacagctgc gggagccacg cgcgacgatt gggcccgtta ctgccaggac catagctacc
     2881 gcgataaaag gcctgccact ccgggatccg cgtacgcgac ggctccaagt aacccgtcat
     2941 catcgcgcgc agtggctcga ccgcgaggcg atatggcaag gcgcccggca atgaacccga
     3001 gaccaaccaa gtcgtctccg tcaagggcaa gcccagcaac aacgagtgag gcggggttcg
     3061 cttgacgcta gctgttctct gcgctcctgc ctttgccgga gcagcccaag tcatcgacca
     3121 ggcgcagagc gcaacggcca tcccacgcga aacgctcctt agaggcagac gactccaaaa
     3181 ccgagcacga ccgttgacca cgccttggta gctttggcga caaagactcc gaacgtcaac
     3241 cgtcagggtt catcgacgag gtcactctcc tcattcggac acattgccgc ggaagctctc
     3301 gctcgcgttc ccgaacgccg ggatcccaca gcccgaccca cttcgtctgg cgcgtcacga
     3361 acatgtcggg agctgcagcg cggagaggct tctcgagcgc agacaggcga cacccggacg
     3421 aatgcctcgc ggggagcgag aacgaccaga cgagtgggta agcgaagcaa aaagagctcc
     3481 actcggcaac gcgcgctacc tgcaagccga cccgagcaac gagcttcgag agcttgcggc
     3541 gctcgggctt accgagcgag ccagccgact tcagctgtcg aagccggtgc ggagcagctc
     3601 gaggaaggtg tcgtacgctt ccttgacctt ggcttcctcg ccctcgaggt actcggtcac
     3661 gctcagacca ttgagcaccg cgagaatgag cgttgccaga gagtcggcca tggccggccc
     3721 agccttttgg gtcaccgggg cgatggcgtc tcgaatcacc tggcgggcat cgcgctgcag
     3781 accggatgcg cggcgcttca gctccgcatc ccgccgtgcg gcgctccaca cctcgatgtt
     3841 gaggccggtg cccgtcttgt tgttctggat cgcgtcccag agcgcacgca gcgcgtcggc
     3901 tgcttgctgc ggcgacccgt caccgttgcg aatcgcttcc ttcagacgat ccaggtagcg
     3961 atggtaggtg aagctctgaa cctcgtgaat gagggcagat ttgctcgcaa agtagtagtg
     4021 gacgatgctc ttgcgtaggc cgagctcctt gccgatttcg gcgagggtcg tggccgtgaa
     4081 ccccttgcgg gcgaagcact tggctgcggc cgacaagatc gaagccacac gggggttatc
     4141 ccagtcggcg ctccgcgccg agccgttctc ttccgttggt ttgcgagcgc ttgcgacagt
     4201 ttctgtgttc atcatggttc gttgctgtac cgaccagccg atatggtgcg gtctcacctt
     4261 cccggtcggg ccaccgccct ctcccggggc ggcgttcttc gcgcaggtcg cccgacctgt
     4321 caagtccgct cgtccgatct cgactttttg cgggactttt tgcccgtctc cgaagtccga
     4381 ccaggccgtt cggccgtctg ctcgcgcgac ttgtaaagag gcatgccggc ttcttccgtc
     4441 caacccgctt gcccaaatcg cccaggttcc cggttctcag cgaaactgct cggctttcct
     4501 tcatcactgt cgccgagcgt catcacgagg agctcgtccg ccaggtactc cggatgagga
     4561 tcgtcgacgg ccttcttcga atcagagtcg gcgtcttttt cgccagtgcc cacgcgcacg
     4621 atctgggtgg tagggggata cacgtcgtcc cagcgctccc gaacggcatg cagaccgtcg
     4681 cgcacgatgc ggtaccggcg caacttgaag cctggcaccc cacgttggcc gagcacgcgg
     4741 gtcccactcg gcaacgactt gtccggccgt tccacttctt cgtacgggat ggagtccagc
     4801 acgcgccgga ttagcgtcac cgtcaggggt ttcttgggcc cgaggatctc cgcgcgcacc
     4861 acgccattct tgacggtctc gtgcagcacg accgggaaag ggaatgggtt ccggatgcga
     4921 aagttgatcg tgggatacac gaccgtcgcg tccagcccca tcttgatata cgagcttggg
     4981 cgcgtgtgcg ggtaacgctc gacgatttcc agaccgccga agaaagcagc cccgtgcaac
     5041 gtcccggaga tttggcaggt gcccccgccg atgccgtcca cgagctcacc ctccgcgatt
     5101 acgggtgcga ccttgtagcc gttggcttcg tcgcgcggtc ccacgaccgc attgaaatcg
     5161 aagatctctc ccggtaacag cacatgcccg tctaggcgcg acgccgcgag ccgcaggttg
     5221 tacgtgcggg cctcttgccg ctgggagcga tcgtagttgg tctcgaagta acctacgact
     5281 tcgctgaact cgacgttacc gagctcgctc gcgagccgcc gcggtttctg ctccaaaaac
     5341 accagcggca cgcggtcttt ccccgcgcgg agcgctcgtt cgatcgcctc gagtgaggca
     5401 tcgacgtcga gctttcggcc gtaaacttcc ttgatcagct cccgcttctc cagatcgagg
     5461 cgggcatcgg ccggcaggcg atccagctcg tctttcaggg caaacaagcg cgccaacgcg
     5521 gtgctccgat cgatcgcaat cggcagcggt aaggcgacct ggctcatgac ttcgaccgcc
     5581 ggcaagttcc ggtacagcgc gctcgtacgg tctcggctgt cccgaaccaa gcgggcgagg
     5641 tgcaccttgt ccagctccac gccgaattgt cccaggtaga tctctcgctc gctcttgtcg
     5701 gggagcacga gccgaaaacg acccgccaga tagctgcgcg cgcgcgccag agcggcgcgc
     5761 tgagcgtccg gaagattcgg ctggggcaag gactgcccga gaaaaagcgg tgtggcgcga
     5821 ttggcaagtg cgtcctcggg tccggcgctc cgaggtagca agaaaaaacc cgacacgatg
     5881 ccggccagaa ttgctaccgc cagggcggca aacgtgcgtc ggctgggaga aaagcgtgac
     5941 ggcatcggcg cccgctctct tttccccaaa aggcgcgcgg cgcaagttgc aacaccggaa
     6001 aatcctgaga cccggtcgtc gtcggctagt tcactcggga accgaggccg cgcccgcgct
     6061 cacctagctc tcgcatagcg aggtgataag gctcggtgat agacctgcgg cgcaataagg
     6121 tgaccgaccc gaccccgcct ccccagacac cctcgccacc gtccgccgtg gatccgctcg
     6181 agctcaagct cgcttcattg ccaactcagc ccgggtgcta cgtgtttcgc gatcgagctg
     6241 gcgcggtgct gtacgtcggc aaggccaaga gcttgcgcgc ccgcgtgcgc agctactttc
     6301 aggcgcagag cggagacact cgggccttca tcccgttctt gcgtgaagag gtcggtgact
     6361 tcgagacgat cgtggccgcc accgaaaagg aggcgacgat cctcgagaat cacctgatca
     6421 aggagcgcaa gcctcgctac aacgtcaagc tgcgcgacga caaggagtac ctgagcctaa
     6481 gaatctcctc ggagcatgag tggccgcgcc tcgaactcgt gcggcgaccg gaacgggatg
     6541 gggctcgcta cttcgggccg tatcactcgg ccacggccgc acgccgcact ttgaacgtgg
     6601 tcgagaagca cttcaagctt cgaacctgca cggatcgcga gctcagcagt cgtaagcggc
     6661 cgtgcttgga gtatcagatc aaacgctgcc cggggccatg cgtatacgag gtagatcggg
     6721 ccgcctacgc cgcccaagtt cgtgcggtcg cgctgttctt ggacggaaga cacgacgagc
     6781 tcgtgcgcga gctcgaggag cgcatgcgtg aggcgagttc gcgcatggag ttcgagctcg
     6841 cggccgcgta ccgcgatcaa ctgcaagcgg tgcagcaggt tcgccaagct cagcgcgtcg
     6901 cggccgtgac ggatagagat caagacgtgc ttggcattta ccgcgagggc gatctcgtcg
     6961 agctcacgtt gatggtcgtg agaggcgggc gtgtgattga cgtcggcagc ttctcgaacc
     7021 ggcgggtcga ggtaccagac gatgaggtca tggcctcgtt tctgcgcgag cactacggcg
     7081 aagggggtgg gggtgaagcc ctgattcccg acgagatttt gctgcccgca ttgcccgaag
     7141 gggtagacgg cgtccgcgag tggctcgacg aacgccggca agccttggcc aaagaacggg
     7201 gcgagcgcgc gcctcgtcgg atcgaaatca tgctgcctat gcgcggcccg cgtcgcgccc
     7261 tcctcgacct cgcgctgcaa aacgccgagc acgctttcaa ggagaagcgc cgcacggcgg
     7321 acgacatcga agagcggctg ctcaaagtcc aagcccgact ccgtctgccg agcgtgccgc
     7381 accgcatcga gtgcgtcgat atctcccacc acgggggtag tgataccgtc ggagccgtgg
     7441 tcgcgttggc agacggcacc cccgacaaga agcggtaccg cacttaccat gtgcgaggcg
     7501 tggcggaggg agacgactac ggcgcgattt atcaggttct ctcacgccgc ttccgacgcg
     7561 ggctcaaagc gcaagacaca agctccgagg ccgaggacga gacagcgtgg gacctgccgg
     7621 acttgttcgt ggtcgacggc ggccgcggcc agctaggggt tgcgctcgcg gctgctcacg
     7681 acctggggtt gcacacgtta cccatcgtgg gcttggccaa agagcgcgaa agcctcacgg
     7741 gtgacaagca agtcgaccgg gtttacctgc ccgggcaaaa aaatcccatc ccactgcgtc
     7801 ccaacagtcc cgaactcttc atgttggctc gggctcgtga cgaagctcat cgcttctcga
     7861 accgcggccg caaaaaagca gggcgacgtc gcacctggag ctcgcgtctg gaagatgtcc
     7921 cgggaattgg tccgaagact cgcaaggcct tgctccgtca cttcccttcg gttcaggcga
     7981 ttttcgaggc gaccgaggag caactgcgcg aggtcccgac gctcactcgc aagcacatta
     8041 cggcgttgct cgcggcgcgt gcagagccgc cgaacatttc gggtgagccg gatctcgatg
     8101 ggggtgacac cgagctggaa gacgccgagc tgggagactt agacgttgaa gaggagacgc
     8161 ccgccgagga tgcagatggc gtggaacccc ccgagccaga acctttgagc aacgcccgcg
     8221 atgtgcttgg gactggcaac tgagttgccg gtctgagtgt aggtttgcca atggcgttgc
     8281 caagcgtctg gcgtttggcc gaacgacccg tgcggcatgt gctttgctgc tgcggagtcc
     8341 agagccgctt gcctccggct tgcttcgcgt tctcatccct gaagtttctc ttccgtggtt
     8401 tcggctacga ttgacaacga gccggccaaa aacctaacct cgcggcgccc tggccgaggt
     8461 cgatcccgcc ctcgccgtgt ggccctcccg agttccaatc gattcggttc ccttctgcga
     8521 tctgatcggt ctcggggaac ggctgcacta ggagtccttt caatgatgct gaagtcccaa
     8581 gcacctacca agcccaatgg agtcctccgt tggctgcgcg ttgccgcagt cggcgtagcg
     8641 gccgcagcgg gagcaacggg gttgacggtc ggctgtctcg accgccccgt gaatccccaa
     8701 gatcccaaga ccagcaacgt ttacgtcgct gaaattcgcc agacggcggt cgataagatc
     8761 gacctgctgt tcatgatcga caactcggcg tcgatggcgg acaagcagga aatcctcggc
     8821 gatgccgtgc cggttctgct cagccgcctc atcactccgg tgtgtgtcga cgagaagacc
     8881 ggg
//
LOCUS       DASTKU010000506         6415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_543011, whole genome shotgun sequence.
ACCESSION   DASTKU010000506 DASTKU010000000
VERSION     DASTKU010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543011"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VFQ61_17445"
     CDS             <1..325
                     /locus_tag="VFQ61_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956299.1"
                     /translation="SNYGAIQSPIPVMYIQGKMDNQRNSNGSEVVARFTAVNKCSTMT
                     MPHSGVDSCKSGSVQVEPGCVTYQGCTQPTIWCSHNDPQYSNTNHGWPCFATKAMYDF
                     FTGLP"
     gene            complement(510..1748)
                     /locus_tag="VFQ61_17450"
     CDS             complement(510..1748)
                     /locus_tag="VFQ61_17450"
                     /inference="COORDINATES: protein motif:HMM:NF018880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956300.1"
                     /translation="MRNPSKTQVSLPWAAMICITAWGCSSANENGNPGLGESPGSTGG
                     ASGLPATAGMTSGGISGTTMSSGGSPSFGGASGATATSATGGSVPAGGTSSASNGGAA
                     PNGGKTSAAGGALAGSGGASAGAPTTTSGGSNAGGATSPSGGVPGSQCTVGSWAMADP
                     AAAGPFATITETNVGPTAGVGSDGAPVAFTLFRPKELGTTGLCHPVVTWGNGTGATPS
                     LYKTLLNHLASHGFVVIASDSPNVADGSPPPMVAGVTWLIEQNALPASPLYQRIDVTH
                     VGATGHSQGGFATSVAGGDSHVTTIAPLCGASPQRNLHGPALVFCGGMDTTVPCSTVQ
                     RAYDSTSNQPVMLASYLSADHANWITFRGSKPSPVEVAVTAWMRVHLMGDTTLRSWFY
                     GADCKLCADTAWQVSKKMMD"
     gene            complement(1911..3164)
                     /locus_tag="VFQ61_17455"
     CDS             complement(1911..3164)
                     /locus_tag="VFQ61_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ricin and poly(3-hydroxybutyrate) depolymerase
                     fusion"
                     /protein_id="HET9956301.1"
                     /translation="MKCLQIDHAAFALSVFVLGCSSSPVPELGAQGGGASLSGGASST
                     TSSGGVTSAAGAPTSTSSGGATSAGGAPGSGGAPSAGGATANGGAHSGGLLSTGAAGA
                     TMSTNGGRAGGGGAPSGGRSNAGGRSSGGTAAGGTAAGGMAGASTAGGGGGGSTGGDV
                     PKTEGCGATSWPTSKDSYTIDVAGTSRSYILRLPNNYDPNHPYRLILAYHWLNGTSQN
                     VANGGGATGKPFYGLWDLAEGSTIFVAPQGINNAWPNSSGRDVEFTRVLLAELSKQLC
                     IDKTRIFAEGFSMGGSMSYALACAMGDVVRAVAVHSGGPMSGCVKHDKPVPYFMTHGT
                     KDSVCTYPEYGVPQLNDFAKINGCTAQSLPTPSGNTPSCVDFAGCKAGYPVRACIFVG
                     DHTPSPPSPQNTWVPAESWKFLSQF"
     gene            complement(3896..5221)
                     /locus_tag="VFQ61_17460"
     CDS             complement(3896..5221)
                     /locus_tag="VFQ61_17460"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET9956302.1"
                     /translation="MTQRLLPLSLGSAFRHGTLLLAFTTLAGCGNDPASSPWTATGGV
                     PATGQGGSTAASGGSATTGGVGQAGSSSGGQATGGSPVTTGGATGSGGAPATGGTSTA
                     STSGGTSSTGPSGGSAGAGGSPMGGTSAQAGGGGKAGGNGGMSSDGGRTSSGGAGGAG
                     AGGASPTGGSGGGGSQTCPTPGLKAGDTTKTIQVGSKSRTYVLHVPSAYDGSKAVPLV
                     VDFHPLGGSGPNERSSSPYPAQTDSEGVIMAFPSGEKGPSGGAWNVGPCCVANVDDVA
                     FAKALVEDVKKSACIDSKRIYAVGFSMGGGMSHYLACHAADVFAAVAPAAFDLAKENV
                     DACKPARPLTVVAFRGTSDPIVSYAGGYSSVVQGMPITFLGAKATFEKWSQLNQCTGS
                     PSAEDGKGCSTYSTCKDGVQVTLCTKQGGGHEAGNASVAWPILKKYTMP"
     gene            5430..6086
                     /locus_tag="VFQ61_17465"
     CDS             5430..6086
                     /locus_tag="VFQ61_17465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9956303.1"
                     /translation="MPLRAIGLMSKRRAQVSLILVGGTNRREISDAELSRGLVSGEGW
                     AINATWHRFAPIVLTLAERCLGSRSEAEDVTQEIFLGVFRRVRVLREPEKLRSFVYSF
                     AVRALKSELRRRKVRAWLSFHAPEAIVDLSSRSLDMESRDLLRRFYALLDRLAPRDRL
                     IFSLREVESMTVDEIAGTMEVSLSTVKRSLRHAEARLSGWIKADPELASALDTERWVR
                     "
     gene            6083..>6415
                     /locus_tag="VFQ61_17470"
     CDS             6083..>6415
                     /locus_tag="VFQ61_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956304.1"
                     /translation="MTPPPDHPPEDGRALGALTKLAREAAARPTPAELERGLHALFGR
                     MASRELQRARVLRVSLLALVASVGAGIGLHWVAFRQPDGAHTAPGYRIHGGSVLEGGY
                     LRDNGSDGM"
BASE COUNT         1153 a         2063 c         1979 g         1220 t
ORIGIN      
        1 ttcgaactac ggtgcaattc agagtcccat tcccgtaatg tacatccagg gaaagatgga
       61 caatcagcgc aacagcaacg gatcggaggt ggttgcgcgt tttacggctg taaacaagtg
      121 cagcacgatg acgatgcctc actcaggagt agactcgtgc aagagcggta gcgtgcaggt
      181 ggagccgggc tgcgtcactt accagggttg cacgcaacct acgatttggt gttcgcacaa
      241 cgatccgcag tacagcaata ccaatcacgg ctggccctgc ttcgccacca aagcgatgta
      301 cgatttcttc actggcttgc cgtgagcccg tcaatcctgc tctgccgagc acgaatccgt
      361 tcgtggctcg gcagagtatg aatctcgcca gcctctgctg ctctgaactg ctgatcgccg
      421 ttgctcggga ggaggcggcg actgcgcctc cttttcctcc gaccgatcgg gattcgtacg
      481 gatcgcgacc agcacccgcg agtccaggtt caatccatca tcttcttgga gacctgccag
      541 gccgtatctg cgcagagctt gcaatctgcg ccatagaacc aggaacggag cgtagtgtcg
      601 cccatcaggt gtacgcgcat ccaggccgtg acggcaactt cgaccgggct cggcttgctt
      661 ccgcggaacg tgatccaatt agcgtgatcc gcgctgagat aactcgcgag catcaccggt
      721 tgattcgagg tgctgtcgta ggctcgttgc acggtgctac agggaacggt ggtatccata
      781 cctccacaaa acacgagggc agggccgtgc agattacgct gcggagacgc gccacacagc
      841 ggcgcaatcg tggtgacgtg actatcgccg cccgcgacgc tcgttgcaaa cccgccttgc
      901 gagtgccccg tcgcgccgac gtgagtgacg tcgattcgct ggtagagcgg actcgccggg
      961 agagcgttct gctcgattag ccaggtcacg cccgccacca ttgggggtgg gctgccgtcc
     1021 gcgacattgg gactgtcgga cgcgattacc acgaagccgt gcgacgcgag gtgattgagc
     1081 agcgtcttgt acagactcgg ggtggcgccc gtgccgttcc cccacgtcac gacgggatgg
     1141 caaagaccag tcgtgcccaa ctccttgggc cgaaacaacg tgaatgcgac aggcgcgccg
     1201 tcgctcccca ctccggctgt gggcccaaca ttcgtttcag taatcgtggc gaacggtccg
     1261 gcagcggcgg gatcagccat cgcccagctg cccactgtgc attgcgaacc gggaacccct
     1321 ccggaagggc tggttgctcc gccagcgttt gacccgccgc tcgtggtggt gggcgcccct
     1381 gccgaggcgc cgccggagcc ggcgagcgcg ccaccggcgg cggaggtttt tccgccgttt
     1441 ggcgccgctc cgccattact tgccgacgac gtgccacccg cggggacgct cccaccggtc
     1501 gcactcgttg cagttgcgcc cgacgctcct ccgaagcttg gagacccacc tgaggacatg
     1561 gtcgtacccg agatgccgcc gctggtcatg cctgcggttg cggggagccc gctggcacct
     1621 ccagtcgagc ccgggctctc accgagtccg ggattcccat tctcgttggc cgagctgcaa
     1681 ccccatgcag tgatacatat catcgcggcc cacgggagac ttacctgcgt cttgctcggg
     1741 ttcctcatcg aagcgatctc cacttgccag ggtatgactt gcctggggta cgacttgcct
     1801 ggggtaatga cgcgacgtgt gatgttgcgt ctcgagaata gtggccggag gtgcccgtca
     1861 cgggtctccc tttatgcgaa agttccgtgc gcttacatcg actgcattcc tcaaaactgg
     1921 gacagaaatt tccaagactc tgctgggacc caagtattct gaggtgatgg cggcgaaggc
     1981 gtgtggtcgc cgacgaaaat gcacgctctg accggatagc ccgccttgca tcccgcaaag
     2041 tcgacgcagg agggggtgtt cccgctaggt gtgggcaggc tctgggccgt gcacccgttt
     2101 atcttcgcga agtcgttcag ctgcggcact ccatactcgg ggtaagtgca caccgagtcc
     2161 ttcgtgccgt gggtcatgaa gtaaggaaca ggcttgtcgt gtttgacaca gccgctcatg
     2221 ggcccgccgg aatgaacggc gaccgcgcgc acgacgtcac ccatcgcaca ggcgagcgcg
     2281 taagacatcg agccgcccat gctgaatccc tcggcaaaga tgcgagtctt gtcgatgcaa
     2341 agctgtttgg agagctccgc caggagcacg cgcgtgaact cgacgtcgcg accgctgctg
     2401 ttgggccaag cgttgttgat gccctgtggc gctacgaaaa tggtgctgcc ttcggccaag
     2461 tcccaaagcc catagaaggg tttgcccgtc gccccgccac cattggccac gttttgtgag
     2521 gttccgttca accagtgata cgcgaggatc aaacgatagg gatgattcgg atcgtagtta
     2581 ttggggaggc gcaagatgta agagcggctc gtgcccgcca cgtcgatggt gtagctgtct
     2641 ttactcgttg gccacgatgt cgccccgcag ccttcggtct tgggcacatc ccccccagta
     2701 cttccgccgc ctccgccgcc ggccgtgctt gctccagcca tgcctcctgc agccgtgcct
     2761 cctgcagccg tgcctcctga actgcgacct ccggcgttgc ttctcccgcc actgggcgcc
     2821 ccgccgcctc ccgccctccc gccgttggtg gacatcgtcg cgcccgccgc gcctgtgctg
     2881 agcagtccac cggaatgcgc tcccccgttg gccgtcgcgc cacccgcact tggagccccg
     2941 ccgctgcctg gtgctcctcc cgcgctcgtg gcgccgccgg atgacgtact cgtgggtgcg
     3001 cccgcagcgc tcgtaactcc gccagaggac gtagttgaac tagctccccc cgagagcgaa
     3061 gctccgccgc cttgagcgcc caactcggga actgggctcg aagagcaacc aagtacgaaa
     3121 acactcaatg cgaaagccgc atgatcgatt tgcaaacact tcataacgac tcctctggga
     3181 tgcgagaatg gctgaagtct gtcacggtgg agaaagggca agggaaggtg aacgagctag
     3241 taggcccagt gctggctcgg gtgggtgcac gagcgcacga acttgcaatt gccccgttga
     3301 gaaatagagc agccgcggca taccgcatga ggcgcggcgg tgggcaaagc ggcatacgat
     3361 gccgtcactg cggcaatagt gctttatccg tcatccaatt tcaaatgagc cagaccgcga
     3421 taacgcaggt gaagaatcac cctcgcgggc gcgtcacggg actctcattc agtacgaaat
     3481 tgcctgacgc aagtcattgc ccgaccgaca tgcgcgaaaa gagcgatctc gttattccaa
     3541 gagcccggga tttttctgag aacaacgcgc gtcttgatcg acgtgggcgt gtagctcagc
     3601 cggcgaggcc aactcgacgg gtgggtggtc gtcccggcag tagcactagt acggcaagcg
     3661 cttcagcgct gggtcgagtt gaaacgggct cgacgacagc gccattccac gcggctcaac
     3721 tcgccgattc acacggttct cagcaatcac gcccctcttg tcagactgcc gcctcccgcc
     3781 tcatcgagcg acactcgacc cccacttcga tcaaatgcaa acgtccgaag cgaactgatc
     3841 cgcttcggac gccgcgccta acctcccggt gagttcgact cgccggggct tcacctcaag
     3901 gcatcgtata cttcttcaaa atcggccaag ctacgctggc gttacccgct tcgtgtccgc
     3961 cgccctgctt cgtgcagaga gtgacctgaa ctccgtcttt gcacgtcgag taggtggaac
     4021 accccttgcc atcttcggct gacggggagc ccgtgcactg attgagttga gaccacttct
     4081 cgaacgtggc tttggcgccc aaaaaggtga tgggcatgcc ctgcaccacc gacgagtagc
     4141 cgcccgcgta ggacacgatt ggatccgagg ttccgcggaa cgccacgacc gtgagtgggc
     4201 gcgcgggctt gcaggcatcc acgttctctt ttgccaaatc gaacgcagca ggcgccacgg
     4261 cggcaaacac gtccgctgca tggcaggcca ggtagtgaga catgccgccg cccatcgaga
     4321 agcccacggc gtaaattcgc ttgctatcga tacaggcgga cttctttacg tcctccacga
     4381 gcgctttcgc gaaggccacg tcgtcgacgt tggccacgca acacggccca acgttccagg
     4441 cgcctccaga cgggcccttc tccccgctcg ggaaggccat gattacgcct tcggaatcag
     4501 tttgggcggg atagggtgag ctcgatcgtt cattggggcc cgagccaccg agcggatgga
     4561 agtcgactac gagtgggacg gctttgctgc cgtcgtatgc cgacggaacg tggagtacgt
     4621 aagtccggct cttggaaccg acttgaatcg ttttcgtcgt gtcgcctgcc ttgagtcccg
     4681 gcgtcgggca tgtctgcgac ccgcctcctc cggagccgcc ggttgggctc gcgccacctg
     4741 ctccggcgcc acctgcaccg ccggagctcg tacgtcctcc atccgagctc attccgccgt
     4801 ttccgcctgc cttgccaccg ccgccggcct gcgccgaagt gcctcccatg ggagagccac
     4861 ctgcgcccgc actgccgccg ctcgggcccg tactcgacgt gcctccgctc gtggacgcag
     4921 tcgacgtccc gcccgtagcg ggggcaccgc cgctgccggt agcaccgccg gtagtaacag
     4981 ggctaccacc ggtagcctga ccacccgacg aacttccagc ttgcccaacc cctcccgtcg
     5041 tggctgagcc acccgaggca gccgtgctgc cgccttggcc cgtcgctgga acgcctcccg
     5101 tcgcggtcca cggcgatgat gcaggatcgt tcccgcagcc cgcgagtgtc gtgaatgcca
     5161 acaatagtgt tccgtgacgg aacgcagagc caaggctgag cggaaggagg cgctgagtca
     5221 tgcgcgcacc gtacctgtcc ctactcaggg tgtacgagag ggcaagtttg tccccgagac
     5281 gtggatacta tttcgtgcca gtgggctaac ggcgctgcgg cgccaacgcc cgaatgtgca
     5341 gcgcgcattt tcgcgatgag attgcaaatt ttcgaccgta tccgcgcgat gcgggcgagc
     5401 gcgaaaaatg aaccagaatt cggtgcgcga tgccactgcg ggccattggg ctcatgtcga
     5461 agcggcgcgc tcaggtctca ctcatcctcg ttggaggcac aaatcgccga gaaatcagcg
     5521 atgctgagct ctcacgcggc ctagtttccg gtgagggatg ggccatcaac gcgacctggc
     5581 accgcttcgc cccaatcgtg ctcaccctgg ccgagcgctg cttgggctct cgttccgagg
     5641 cagaagacgt gacgcaggag atcttcctgg gcgtgtttcg ccgtgtacga gtgctgcggg
     5701 agcctgagaa actgcgcagt ttcgtgtact cgttcgcggt tcgcgccctc aagtcggagc
     5761 tacgtcggcg taaggtgcgt gcatggttga gttttcatgc gccggaggcg atcgtagatc
     5821 tgagctcgcg aagcttggac atggaatcgc gtgatctgct gcgccgcttc tacgctctgc
     5881 tggaccgtct ggcgccgcgg gatcggctga tcttcagtct gcgcgaggtg gagtcgatga
     5941 ccgtggacga gatcgccggg acgatggaag tctcgctatc gacggtcaag cgatcgctgc
     6001 gccatgcgga ggctcggctg tctgggtgga ttaaagcgga tccagagctc gcgagcgcgc
     6061 tggacacgga gcggtgggtc cgatgacacc tccacccgac caccccccgg aagacggtcg
     6121 ggcgctcggc gccctcacca agctcgcgcg cgaggctgcc gcccgaccca ccccggccga
     6181 gctcgagcgt ggcttgcacg cgctgttcgg tcgaatggct tcgcgagaac tccagagagc
     6241 gcgagtgctc cgcgtgtcgc tactggcgtt ggttgcaagc gtgggtgctg ggatcggtct
     6301 gcactgggtt gcctttcgtc agcctgacgg agcgcatacc gcgcccggct atcgcatcca
     6361 cggtggcagc gttctcgagg gaggatacct acgcgacaac ggcagcgacg ggatg
//
LOCUS       DASTKU010000507         3535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1882609, whole genome shotgun sequence.
ACCESSION   DASTKU010000507 DASTKU010000000
VERSION     DASTKU010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1882609"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="VFQ61_17475"
     CDS             <1..1329
                     /locus_tag="VFQ61_17475"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HET9956305.1"
                     /translation="RDPWLKRMFLGYAFSIDYRDRRGHAYMLADQEATQRLTKPQTGS
                     CLHCHASIMPLYRELGGGDAMAGFEKTWQFSYQELDKKLHDSGNAHPVSCVDCHDPKT
                     MSVRVTRPGFINGIRALAASDAPVPGLPSIEIWRSGARKEPYDPNRDASRNELRSYVC
                     GQCHVEYYCSSKMPLTFPWGKGLNVDSAEAFWNETKFKDGSDFVDYKHKETGAPILKA
                     QHPEFELWSQGIHARSGVSCADCHMPYMREGASKVSDHWVRSPLLNINRACQTCHRAS
                     EEEMKSRVNTIQARNYELLQRGGAAIVDLIESIVKAKAQGVTEAELAPALALQRRAQW
                     RLDYIAAENSMGFHAPQEAARILGEAADYARQGQVAVLNRGVIPTPPPEASAAASSSA
                     SKPALAQESKVAGKAAASGSPAAPGVAAAPAPAAPAPAAPASPAAPAASH"
     gene            complement(1561..2205)
                     /locus_tag="VFQ61_17480"
     CDS             complement(1561..2205)
                     /locus_tag="VFQ61_17480"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9956306.1"
                     /translation="MKRIGVLGSGAVGETLANGFLKHGYEVMRGSREPVKLESWKSKH
                     GPEAWIGTFAQCAEFGELLVLAVKGGAAESAIDLSGPHISGKTVIDATNPLDETPPVN
                     GVLSFFTGPNESLMERLQRRAPSARFVKAFSSVGNSLMVNPDLGQKPTMFICGNDAAA
                     KRETTGILDQFGWEAEDMGTAEAARAIEPLCILWCIPGFLRNDWVHAFKLLKKG"
     gene            complement(2382..>3535)
                     /locus_tag="VFQ61_17485"
     CDS             complement(2382..>3535)
                     /locus_tag="VFQ61_17485"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HET9956307.1"
                     /translation="ATGGNSVASSSGGNVSASGGATSASGGAVSASGGATTPGGGPNG
                     TSGGSVTVMGGASSVAGATSGGASAGGSDSSTPVGKHGQLAVVGTQLVDQQKQPVQLK
                     GPSSMWLNWESKPFAEDKAGLQWMRDNWKASVVRAAMGVSTTDTMDVDYLSAPEAAQA
                     QVEKIIQNAIDLGIYVIVDWHDGAADTRQDKAIAFFKSIAQKFGSYPNVIYETFNEPT
                     RQAWSTVLKPYHTAVVAAIREVDPDNVIILGTRNWSQFVDEAAADPVAGTNLMYTLHF
                     YSCTHKADIRTRGETALGMGLPLFVTEWGATHADGGTPKNPMVCADEAQLWHDWMNKN
                     KISWAAWKWDQCVDQSCYFKPDIELSTAGNWTDDMLNGHATFVRDRMKE"
BASE COUNT          641 a         1133 c         1107 g          654 t
ORIGIN      
        1 cgtgatcctt ggctgaagcg gatgtttttg ggttacgcgt tttcgatcga ctaccgcgat
       61 cggcgcggtc acgcctacat gctggcggac caagaagcga cgcagcggct caccaagccc
      121 cagaccgggt cttgcttgca ctgtcacgcc tcgatcatgc cgctttatcg cgagctcggc
      181 ggaggcgacg cgatggctgg attcgagaag acttggcagt tcagttacca ggagctggac
      241 aagaagctgc acgacagcgg gaacgcacac ccggtgagct gtgtggactg ccatgatccg
      301 aagacgatga gcgtgcgcgt gacgcggccc gggttcatca acggaattcg cgcgctcgct
      361 gcctcggacg cgccggtgcc cggcctaccc tcgatcgaga tttggcgttc gggggcacgc
      421 aaggaaccct acgacccgaa tcgcgatgcg agccggaatg agctgcgctc ctacgtgtgc
      481 gggcagtgcc atgtggaata ctactgcagc tcgaagatgc cgctcacgtt cccctggggc
      541 aaaggcttga acgtggacag cgcggaggcc ttctggaacg agaccaagtt caaagacggc
      601 agcgacttcg tcgactacaa acacaaggaa acgggcgcgc cgatcttgaa agcgcagcat
      661 ccggagttcg agctctggag tcagggcatt cacgcgcgga gcggcgtctc ttgcgctgac
      721 tgccacatgc cttacatgcg tgaaggcgcg agcaaggtca gcgatcactg ggtgcgcagc
      781 ccgctgctca acatcaaccg cgcgtgtcag acctgtcacc gcgcgtccga agaggagatg
      841 aagtcgcgcg tgaatacgat tcaggcgcgc aactacgagc ttctgcaacg tggcggcgcg
      901 gcgatcgtcg acttgatcga gtcgatcgtg aaagccaaag cgcaaggcgt aacggaggcg
      961 gagctcgcgc ccgcgctcgc cttacagcgg cgcgcacagt ggcgcctgga ctacatcgct
     1021 gcggaaaaca gcatggggtt ccacgctccg caagaggcgg cccgcattct gggtgaggcc
     1081 gcggactacg ctcgccaagg ccaagtcgcg gtcctgaatc gaggtgtgat tcccacgcca
     1141 ccgcctgaag ccagcgcggc cgcttcgagc agcgcgtcca agccagcgct ggctcaagaa
     1201 agcaaagtag ccggcaaagc ggccgcgagc ggtagccctg ctgcacctgg cgtagcagcg
     1261 gcgcctgcac ccgccgcgcc tgcacccgcc gcaccagcga gccccgccgc gcctgcggcc
     1321 agccactaag ggtcgattat cgagtggtca cgaagtgctg cgatcctcgg atcgcagcac
     1381 ttcaagtgtg cgacacctcc ggagctcggc ggctgccgag gcgttgctcg gcgccgtttg
     1441 cgcggcgcga agggggatcg ggccgcgctc ggtggcgcct aggtgtggct cgggagctct
     1501 tcgcgctctt tacatcgcgc ggccaagaaa cgacgtggac cgccgataag cgcgttcggt
     1561 tcagcccttc ttgaggagct tgaaggcgtg cacccagtcg ttgcgcagga acccggggat
     1621 gcaccacagg atacagagag gctcgatagc gcgtgcggct tcggccgtac ccatgtcttc
     1681 cgcttcccag ccgaactgat cgaggatgcc ggtcgtttcg cgcttcgcgg cggcgtcatt
     1741 accgcaaatg aacatggtcg gcttctgccc aaggtcgggg ttgaccatca gcgaattgcc
     1801 cacgctcgag aaggctttga cgaagcgagc ggacggcgct cgccgctgca ggcgttccat
     1861 caaggattca ttcggccccg tgaagaacga gagtacgccg ttgacgggtg gagtctcgtc
     1921 gagcgggttg gtggcatcga tcaccgtttt gccggagatg tgaggtccgg agagatcgat
     1981 tgccgactcc gccgcaccgc ctttgacggc caaaaccagc agttcaccga actcggcaca
     2041 ttgggcgaag gtgccaatcc aggcttcagg cccgtgtttg ctcttccaac tctcgagctt
     2101 caccggctcg cgcgagccgc gcatgacctc gtaaccgtgt ttgaggaagc cgtttgccag
     2161 tgtctctcca acggcgccag aaccgagtac ccctatgcgt ttcatgtgcc gcgatctacc
     2221 acgcgcgtcc tcgacaggcg acacagaagc gtgggctgcg gaatctacgc ggttgccacg
     2281 actcgttgga acgagcgctc cacgcaacgt gacgtcgcgc ggagcgctcg gggccgggcg
     2341 gtccgagggg actcactcgt cctctcctca gcgcgtcagt ctcattcttt catgcgatcg
     2401 cgcacgaacg tcgcgtgccc attcagcatg tcatcggtcc aattgccggc cgtcgagagt
     2461 tcgatgtcgg gcttgaagta gcagctctga tcgacacatt gatcccactt ccacgcggcc
     2521 cagctgatct tgttcttgtt catccaatcg tgccagagct gcgcctcgtc ggcgcacacc
     2581 atcggattct tgggggtgcc gccgtctgcg tgggtggcac cccactcggt aacgaacaag
     2641 ggcagaccca tgccgagcgc cgtctccccg cgcgtgcgga tatcggcttt gtgcgtacaa
     2701 gagtagaagt gcagcgtgta catcaggttg gtgcccgcca ccggatcggc cgccgcttcg
     2761 tccacgaact gagaccagtt tcgagtaccc aagatgatca cgttgtctgg atcgacctcg
     2821 cgaatcgcag cgaccacggc cgtgtgatac ggcttcagca ccgttgacca ggcttggcgc
     2881 gtgggctcgt tgaaggtttc gtagatcacg ttcgggtagc tcccgaactt ctgcgcgatc
     2941 gacttgaaga acgcgatggc tttgtcttgg cgtgtgtcgg cagcgccgtc atgccaatcc
     3001 acgatcacgt agatcccgag gtcgatcgcg ttctggatga tcttctcgac ttgagcctgg
     3061 gccgcctccg gcgcgctcaa gtagtcgacg tccatcgtgt ccgtagtcga gacacccatc
     3121 gccgcgcgca ccaccgaagc cttccagttg tcccgcatcc actgcaaacc ggccttgtcc
     3181 tcggcgaaag gcttgctctc ccaatttagc cacatgctgc tcggcccctt cagctgaacg
     3241 ggctgcttct gttgatcgac gagttgggtc ccgacgacgg cgagctgacc gtgtttgccg
     3301 acgggtgtgc tcgagtccga gccgcccgca gatgccccac cactcgtcgc tccggcgacg
     3361 ctagaggcgc cgcccatcac cgtcacggat cctccactcg tgccattcgg gcctcccccc
     3421 ggagtagtag cgcctccgga tgcgctgact gcgccgcccg atgcgctggt agcgccgccg
     3481 gatgcgctga cgttgccgcc actcgaagac gcaactgagt tgccacccgt tgcga
//
LOCUS       DASTKU010000508         3248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_319714, whole genome shotgun sequence.
ACCESSION   DASTKU010000508 DASTKU010000000
VERSION     DASTKU010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319714"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="VFQ61_17490"
     CDS             <1..1367
                     /locus_tag="VFQ61_17490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9956308.1"
                     /translation="DAQRRITLVNREAEKLFGYARAELLGQPIELLVPEQMRDSHPKD
                     VDTFLANPKKRAMGAGRELHGRRRDGTLVPVEIGLNPIETREGMFTLASVIDISERKR
                     AEAAHERLAAIVESSDDAIISKSLDGVIQSWNAGAERIFGYTADEVIGQTVSMLMPER
                     CIEEERGLLARIRNNERISHFETTRKRKDGREFDASVTLSPIRRASGQIVGASSISRD
                     ITELKRRDAELQRSNAELEQFAYVASHDLQEPLRMVANYTELLAERYRGRLDEKADKY
                     IHYASEGARRMQQLVRDLLAFSRVGSQGKPLYPVSAKGVLQSVIDSLQPLIQSTGAQI
                     EVEPLPTVLADETQLRQLFQNLLGNSLKFRSKAAPRVRVSCRHLGDRYQFSVSDNGIG
                     IDMQYSERVFQMFQRLHDRSEYEGSGIGLAIAKRIVERHGGRIWIESELDRGTTIHFT
                     LKSG"
     gene            1424..1846
                     /locus_tag="VFQ61_17495"
     CDS             1424..1846
                     /locus_tag="VFQ61_17495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9956309.1"
                     /translation="MSKPVRVLLIEDNPADADLTKETLESGRILVDIMVATNGAEALA
                     QLKHRDTLATPLPDLILLDLNLPRIDGRHVLREIKKDNELRRIPVVILTSSDAERDIV
                     QSYELGANCYVTKPVGLQAFQSIVRSLEGFWLSVVRLP"
     gene            1886..>3248
                     /locus_tag="VFQ61_17500"
     CDS             1886..>3248
                     /locus_tag="VFQ61_17500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9956310.1"
                     /translation="MSTHQVLIVEDNLADAELTSELLAAAQVARFNVLRAATAVSAVE
                     TLKSVRVDAVLLDLNLPDSRGLETLRQVKPKAADAPIIVLSGMVTPELLVQARHEGAE
                     EVFDKDESNTPLLSLSVLYVIERTRSRVQQKQLEALLDATADAIVVVSASGEVQYVNQ
                     AALTLFGRKREEFDHVLPGFAVSDGRAAEVTIQTRDEDRICEMRVVPIEWRRQAARLA
                     SIRDITRQRKLEMQLMVSDRMASIGTMAAGVAHEINNPLASVIGNLQLACDTMEQWGA
                     NNKPPGLAVAPSDPPPLPANVTHHQRTEIETEQVLEELYDAREAAERVRQIVRDLRVF
                     ARSEDERREPVDVQRVIESALRMAWNELRHRATVVRRYTRVPPVLATEARLGQVVLNL
                     LVNAAQSIPEGDSARNEVRVSTGLDDQGRIVIAIADTGSGMPPEVQRRLFTPFYSTKP
                     PGSG"
BASE COUNT          646 a         1018 c         1030 g          554 t
ORIGIN      
        1 tcgacgctca gcggcggatc accttggtca accgtgaagc ggagaaactg ttcggctacg
       61 ctcgcgccga gctcctgggc cagccaatcg agctactggt ccccgagcag atgcgggact
      121 cgcaccccaa ggatgtggac acattcttag ccaatccgaa gaagcgagcc atgggcgccg
      181 gccgcgagtt gcacgggcgg cgcagagatg gaactttggt cccagtcgag atcgggctga
      241 atcccatcga aactcgagag ggcatgttca cactcgcgtc ggttatcgac attagcgagc
      301 gcaaacgggc cgaagcggcg cacgaacgcc tggccgccat cgtcgagtcg tcggacgacg
      361 ccatcatcag caagagcttg gatggggtca tccagagctg gaatgcggga gcggagcgca
      421 tttttggcta caccgccgat gaggtcattg gccaaaccgt aagcatgttg atgcccgagc
      481 gctgcatcga agaagagcgg gggctgctcg cgcgaatccg caacaacgag cgaatctcgc
      541 atttcgagac cacccgcaag cgcaaggatg gccgcgagtt cgacgcctcg gtcacgttat
      601 cgccgattcg gcgcgcctca ggccagatcg tgggcgcgtc ctcgatttcg cgtgacatca
      661 ccgagttgaa acggcgggac gccgaactgc aacgcagcaa cgcggagctg gagcagtttg
      721 cttacgtcgc gtcccacgac ttgcaggagc cgctgcgcat ggtcgcgaac tacaccgagc
      781 tcttggcgga gcgctaccgg ggacgcttgg acgagaaggc ggacaagtac atccactatg
      841 cgtcggaggg ggcccggcgc atgcagcagc tggtccggga tctgttggcg ttttctcgag
      901 tgggctcgca aggcaagccg ctctatccgg tttcggccaa aggcgttctg caatcggtga
      961 tcgattcact gcagccgttg attcagagca ccggcgcgca aatcgaagtg gagccgctgc
     1021 ccacggtgct ggccgacgag acgcagctgc gtcagctgtt ccaaaacctg ctcggcaatt
     1081 cgctcaagtt ccggtcgaag gcggcgcccc gcgtgcgcgt gagctgtcgg cacctcggcg
     1141 accgatatca attctccgtc tcggacaacg ggatcggcat cgacatgcag tactccgagc
     1201 gcgtgttcca aatgttccag cggctccacg atcgcagcga atacgagggc agcggcatcg
     1261 gtctggcgat cgccaaacgc atcgtcgaac gtcatggtgg gcggatctgg atcgagtccg
     1321 agctcgatcg cggcaccacc atacacttca ccctgaaaag tggctgaatg ccgcacgctg
     1381 tcgcaccgcg tccgcgagcg gcacccgaac caccaagggc cccatgagca aacccgttcg
     1441 cgtcttgttg atcgaggaca atcctgccga tgcagatctc accaaagaaa ccctcgagtc
     1501 tgggcggatt ctcgtagaca tcatggtggc gaccaacggc gcggaggcgc tcgcccagct
     1561 gaaacatcgt gatacgttgg ccacgccctt acccgatctg atcctgctcg acctaaactt
     1621 gcctcgcatc gacggacgcc acgtgctcag ggagatcaag aaggacaacg agctgcggcg
     1681 tattccggtg gtcattttga cctcatcgga tgcggaacgg gacatcgtgc agagctacga
     1741 gctcggcgcg aactgctatg tgaccaagcc cgtggggctt caagcatttc agagcatcgt
     1801 tcgctcacta gaagggttct ggctatcggt cgtaaggttg ccgtagggca tccaaaaccc
     1861 gagagcgcct gaagaggacg cgcctgtgtc gacgcaccaa gttctcatcg tcgaagacaa
     1921 tctcgctgac gccgagctca cgtccgagct attggccgcc gcgcaggtcg cgcgcttcaa
     1981 cgtgctgcgc gccgccaccg ctgtctctgc ggtcgaaaca ctgaagtccg tacgtgtgga
     2041 cgccgtgctg ctggatctca atctgcccga ctcgcgtggg ctggagacgc tccgacaagt
     2101 aaagcccaag gccgccgatg cccctatcat cgtgctctcg ggcatggtga cgcccgagtt
     2161 gctcgtacaa gcgcggcacg aaggtgcgga ggaggtgttc gacaaggacg agtcgaacac
     2221 acctctactc tcgctgagcg tgctgtacgt gatcgagcgc acgcgctcgc gggtacagca
     2281 gaagcaactc gaggccttgc tggacgccac ggcggacgcc atcgtcgtcg tctccgcgag
     2341 cggtgaggtt cagtacgtca atcaggccgc gctcacgttg tttgggcgca agcgggagga
     2401 gttcgatcac gttttgcccg gcttcgccgt gtccgacggc cgcgccgcgg aggtcacgat
     2461 tcaaacccgt gacgaggatc ggatctgcga gatgcgcgtg gtgcccatcg agtggcgacg
     2521 gcaagccgcg cgcctggcgt ccatccgaga catcactcgc caacgcaagc tagagatgca
     2581 gctcatggtc tccgaccgaa tggcgtcgat cggaacgatg gcggcggggg tcgcacacga
     2641 gatcaacaat ccgctcgcat cggtgattgg caatctgcaa ctcgcctgcg acaccatgga
     2701 gcagtggggc gccaataaca agccgcccgg gctcgccgtc gcgccgagcg atccgccgcc
     2761 tctgccggcc aacgtaaccc accaccagcg gaccgagatc gagaccgagc aggtcctgga
     2821 ggagctgtac gacgcgcgtg aagccgcaga acgcgtgagg cagatcgtcc gtgatctacg
     2881 cgtgttcgct cgttcggagg acgaacggcg cgagcccgtc gacgtgcagc gcgtaatcga
     2941 gtcggcgctg cgcatggcgt ggaacgagct gcgtcatcgg gcaacggtcg tgcgccgcta
     3001 tacccgcgtt ccgcccgtgc tcgcgaccga ggcgcgcctc gggcaggtcg tgctgaatct
     3061 cttggtgaac gccgcgcaat ccatcccgga gggggactcg gctcgcaacg aggtgagagt
     3121 cagcaccggg ctcgacgacc aggggcgtat cgtgatcgcc atcgccgata cgggcagcgg
     3181 catgcctccc gaagttcaac ggcgcctatt cacccccttt tacagcacca aaccgccggg
     3241 ctcgggca
//
LOCUS       DASTKU010000509        12008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_765503, whole genome shotgun sequence.
ACCESSION   DASTKU010000509 DASTKU010000000
VERSION     DASTKU010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12008
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765503"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /gene="pth"
                     /locus_tag="VFQ61_17505"
     CDS             complement(<1..625)
                     /gene="pth"
                     /locus_tag="VFQ61_17505"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET9956311.1"
                     /translation="MLVVGLGNPGPRYEKTRHNIGFMLVDRLAERAGSAQFRERFHGH
                     YAEVRFQNEKLGLLKPLTFMNDSGRSVQAALTASYLDPSSLLIVHDELDLPRGELRLK
                     QAGGDGGHRGLRSISGLLGPAYLRLRIGIGRPPPEFRGEVSDFVLERFPPAELVEFDS
                     VLDRAVEAVATLVSRGIQAAMNATNQRAIPAKNPAPDKNLAPTKSLGP"
     gene            complement(625..1281)
                     /locus_tag="VFQ61_17510"
     CDS             complement(625..1281)
                     /locus_tag="VFQ61_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET9956312.1"
                     /translation="MEIIKIDASRRNDKGKSEARRLRNAGKIPAVAYGKDLAAQSLAI
                     APSALTKVLNSVHGRNTVVELDVDGQSKVTALVRDYQYHPVSREFLHADFIQISLDQE
                     VDVEVALELTGKAQGVVLGGTLRQVFRSVPVRCLPEKIPVKLVHDITALGLDGHVAAK
                     DLVLPEGVKVRLAPERTLVSIVKEKIEREEEVAKPGAAPAAAPAAAGKAEPEKKAEKK
                     "
     gene            complement(1416..2363)
                     /locus_tag="VFQ61_17515"
     CDS             complement(1416..2363)
                     /locus_tag="VFQ61_17515"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET9956313.1"
                     /translation="MALKRVCIFTGNANPELATEIARVLDTPLGEAKVSRFSDGETFC
                     EIRENVRGLDTYVVQPTCSPVNDNIMELLIMCDALRRASAAGITAVIPYYGYARQDRK
                     VAPRTPITSKLVADLLVAAGVTRVVCVDLHAGQIQGFFNIPFDHLHALPVFLDDYLRP
                     RFNAETVFVSPDAGGVERARAYSKRLGAMLAIIDKRRDRPNVSEVMHLIGDVRGRDCI
                     IVDDMIDTAGTLCNAARAVMEHGARRVVACATHGVLSGPAVQRIEESPLEEVVVTNSI
                     SMSEEARASKKIRVLSIARLLGEAIRRIHNSDSVSSLFV"
     gene            complement(2639..4141)
                     /locus_tag="VFQ61_17520"
     CDS             complement(2639..4141)
                     /locus_tag="VFQ61_17520"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9956314.1"
                     /translation="MVAARLFDPALGRQRVERYELIGEIASGGMATVYLARLTGMGGF
                     QRFVAMKRLHPHLASEAEFVEMFLDEARVAARIHHPNVVPILEVGASLVGYYLVMEYV
                     EGDTLARLLARAGSVGRRVPAAITLRVALDMLNGLHAAHELHEDDGRPIELVHRDVSP
                     QNVLVGSDGIARITDFGVARAATRLSGTRVGQLKGKIAYMAPEQAHGDERLDRRADVF
                     AAGIVLWEALTGKRLFKADTEAATLSRVMTERVPPLLESVPELGRPLSDAIAQALSRD
                     PRHRFSTCAAFADALETAAAYGPRIATSRELASYVEEVMGDEIARQREAVRLWLSRTE
                     SIPPQSSRYVSSMPPSSSVSAAAVSLPHATGILGEITVPDAPRRSRWAWVGLFAVLLA
                     GSGIGGYFGVRFFRAPEPKAGVLAASPGPSAAVAPAAPSANAAPAPSPAPTGSVETPV
                     YAARAGSAVAPSATAVPARPRSAATPTAPVPVRPRKKKGEGDDLPENPYR"
     gene            complement(4147..5211)
                     /locus_tag="VFQ61_17525"
     CDS             complement(4147..5211)
                     /locus_tag="VFQ61_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956315.1"
                     /translation="MSSSLGLCAVPQVAHAAHEPAPAQIREAANAFDQGRAAYKKENW
                     VEAAEYFEHAYDSVPSARALEYAIRARDKAGQLDRAATLAALAERRYSDEASLQKLVP
                     GILARARESLYELFVTCPEPCDLAVAGKIVPGEPELQRRIYVYEGRQSVRAAYGGDRN
                     ESREVTARSGGKGDLSFDSQVREDTNEGSLETTDPSADDQPAKTPTPKPAQDAARSAS
                     GLPQGVFFTGVALSSVGVLVTTLSGIDTLKNPGKDRIRNECEQGDTSCPVYQDGLDRQ
                     RRTNVLLGVTAGLSIATTLVGVFWTDWSSRPDSEPSEEYVSGRRVRRSASAARPRSGA
                     RVTPVLSLGQGAFVGAQGSF"
     gene            complement(5821..7173)
                     /locus_tag="VFQ61_17530"
     CDS             complement(5821..7173)
                     /locus_tag="VFQ61_17530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET9956316.1"
                     /translation="MSKFEFKLPDIGEGVAEGEIVNWLVQEGDAVSENQDMVEVMTDK
                     ATVTIGAPKAGKVAERRFKVGDTVPVGEVLVVFEVGGEGAPAAKPEPAAKPEPAAKAE
                     PAKAAPVATAVGDIKESLPGISAMPAAKPTAAPAPRANDYFNDKPLAAPATRKLAREL
                     GVDLRNIEPSGGAGRVTREDVEKAAQRPSGAAAQPAPSEAPAARAPLPEAPRPAARET
                     DERMPIRGLRKRIWESMARSKHTAAHFNYVDECDVGPLIALRDRAKPHAERSNVKLTF
                     LPFIVKAVVAALKRHPRLNAVVDESAHELVLRKNFDIGIAAATDAGLMVPVIRDADRL
                     SLVEIAREIDRLGQLARSGKIQKEDLGGSSFTISSLGKLGGFLAPPIINHPEVGIMGI
                     HAIKRKPVVRGDQIVIGDVMMLSFSFDHRIIDGDVGAAFAQEIISYLQEPDRLIVEMS
                     "
     gene            complement(7213..8187)
                     /locus_tag="VFQ61_17535"
     CDS             complement(7213..8187)
                     /locus_tag="VFQ61_17535"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET9956317.1"
                     /translation="MPQMNMVQAINDALRIAMRDDDRVVVLGEDVGKVGGVFRVTAGL
                     FDEFGDDRVIDTPLSENGIVGSAVGMALYGLLPVVEIQFADFIWPAYDQIVNELAKYR
                     YRSGNEYPAKVVIRTPVGGGIRGGHYHSQHPEAAFIHVPGLKVVCPSNPYDAKGLLLA
                     SIADPDPIIFFEPKRVYRAAKGDVPIGAYTTPIGSAAVVRPGKHVTVLAYGAMLYEAL
                     DAAAKAAAKGVEAEVIDLRTLWPVDIDTVIESVKRTGRLVVVHEAPKTCGFGAELVSL
                     VTERAFYHLEAPPVRVTGFDTPFPYTLEMEYMPLSHRILPTLLDAARV"
     gene            complement(8209..9345)
                     /locus_tag="VFQ61_17540"
     CDS             complement(8209..9345)
                     /locus_tag="VFQ61_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET9956318.1"
                     /translation="MVATTRQRSESTPPESGMLRVLNDDLTLDPAYDPGLSTEQVLDM
                     YRHMVTTRLLDERLVALQRQGRVGFHVGSLGEEAAIIGSAYAMRKTDFLFPCYRELGA
                     ALMRGLELQVFVHNMFGTAKDVVRGRQMPDHYTCRRVGFGSISSPVGTQITQAVGFAW
                     AAKIKKQDVATLVYFGDGATSSSDFHSGMNFAGVFKIPTVFLCRNNGWAISVPTARQS
                     ATRTLAEKAAAYGVAGVQVDGNDLFAVVSAVRRAVERGVRGEGPTLIEAITYRMGGHS
                     TNDDPNRYRGSEALEPWTSRDPIGRVRAYLEKRDAWDGAAEERLIQDIDQRFREAVAV
                     AERTPPPPLESMFDDVYERPPWHLVEQRNEVLSGPRAPAKHGNS"
     gene            complement(9365..9955)
                     /locus_tag="VFQ61_17545"
     CDS             complement(9365..9955)
                     /locus_tag="VFQ61_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956319.1"
                     /translation="MAIMQALDVHLITRVVRQLGLPPSSQAASAGASARSIWAATASS
                     YAYGAECDEFTQPTGFCPHAALLFEAILEAALLVAGSAARLPRQSEAFKKIVVFASGG
                     LLREPQISGLFSMLDERLQREGVQRRLDSIASAAERPEQAREVLRVASLFGVAEAAGE
                     LGTEKLDLLRALTKRLGLPAQDLQTSVAEAKNVLFR"
     gene            complement(10045..11235)
                     /gene="lipA"
                     /locus_tag="VFQ61_17550"
     CDS             complement(10045..11235)
                     /gene="lipA"
                     /locus_tag="VFQ61_17550"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET9956320.1"
                     /translation="MAERSVSEHGPSEEGDLCERGDAAASGTRAKSLSGPASAAKAPA
                     FKEPKPGWLKVRAPGGDNYTRLKETLRGLDLYTVCEEARCPNVGECWASGTATVMLLG
                     HTCTRGCRFCAVTTGNPRGAYDPREPVHVARAIEKLGLRYVVLTMVDRDDLLDGGASQ
                     VAETVRELKQRVPELLVETLVGDFGGRRADIDVVLGSRPDVFAHNIEVPRRLTPRIRD
                     QRCSYDRSIDVLRHAKQAAPERLVKSSIMVGIGETDDEIYEAMSDLRSAGVDIVTLGQ
                     YLRPTPKHAPVERYVEPAQFDAYAGQGRALGFAYVASGPLVRSSYHAAEGFVAAYLGK
                     PLAAAAGTASNVGRAHAEAERLTQAAAAVGSELAAPPSLLERAARSAPSAEQIIDVRS
                     LVRR"
     gene            complement(11235..>12008)
                     /locus_tag="VFQ61_17555"
     CDS             complement(11235..>12008)
                     /locus_tag="VFQ61_17555"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HET9956321.1"
                     /translation="RFVQRAIDSEVARQTALLESGQKVTQETRSFDPDTGLTKTLRSK
                     EEAHDYRYFPEPDLRPLVLDDVFVRKCESSLPELPTAVRNRWLSQGIAAPAAATMSQH
                     PGYVRFFDQLCAVFPNPVKAANFFANDVLRGAKTRGLEATFTVTPEQTAELLALVEKG
                     DISGKQAKEVFSAIENTTRMPSSIVEERGMKVVSDVDSLRRVCQALIEKFPSQAESLR
                     SGKKGVLGFFVGQAMKETGGSANPKLVNELLESLILGGS"
BASE COUNT         2150 a         4001 c         3701 g         2156 t
ORIGIN      
        1 ccgggcccaa gctcttggtc ggagccaagt tcttgtccgg agccggattc ttcgccggaa
       61 ttgcgcgctg gttggtggcg ttcatcgccg cctggatgcc acggctaacg agcgtggcga
      121 ccgcctcaac ggcgcggtcg agcacagaat cgaactcgac gagctccgcg ggggggaagc
      181 gttctagcac gaaatccgaa acttcacccc gaaactcggg cgggggccgc ccaattccga
      241 ttcgaagcct cagatatgcg gggccaagca accctgaaat cgaacgtaga ccacggtgac
      301 caccgtctcc acccgcttgt ttcaggcgga gttcgcctct cggcaggtcc agctcgtcgt
      361 gcactatgag caaagaactc ggatcgaggt acgaagcggt gagcgcagct tgtacgctgc
      421 gcccgctgtc gttcatgaaa gtcagcggct tgagcagacc gagcttctcg ttctggaaac
      481 gcacttcggc gtagtgcccg tggaatcgct cgcgaaactg cgccgagccg gcgcgctccg
      541 cgagcctgtc caccagcatg aagccaatgt tgtgccgggt cttctcgtag cggggacccg
      601 ggttgcccag gcccacgact aacatcactt cttctcggcc ttcttttcgg gctcagcttt
      661 gcccgccgcc gcgggtgccg ccgcgggagc cgcaccgggc ttcgcgacct cttcttcacg
      721 ctcgatcttc tctttcacga tcgagaccag cgtgcgctcc ggcgcgaggc gaaccttcac
      781 gccctccggc aggaccaagt ccttcgcggc cacgtgacca tcgaggccga gcgccgtgat
      841 gtcgtgaacg agcttcaccg gaatcttctc gggcaagcag cgcacgggta ccgaacggaa
      901 cacctgacgc aacgtgccgc cgagcaccac gccctgggct ttgccggtga gctcgagcgc
      961 tacttccacg tccacctctt gatcgaggct gatttggatg aagtccgcat gcaagaactc
     1021 gcggctcacg gggtggtact ggtaatcgcg aacgagagcg gtcaccttgc tttgcccgtc
     1081 cacgtccagc tcgaccaccg tgttgcgacc gtgcacgctg ttcagcacct tggtgagcgc
     1141 gctgggagca atggcgagag actgcgcggc caaatccttg ccgtaagcaa cagccggaat
     1201 tttgccggcg ttgcgcagcc tacgagcttc gctcttgcct ttatcgttgc gacggctggc
     1261 gtcgatcttg atgatctcca ttttcctttg aactcctgaa cccttctatt gaggacccca
     1321 gctgaactct tgtcggcgtc agcgaatctg gcgcttgttc ctctcgcctg ccgtcacaca
     1381 actttcgggg ggtgcattgc tagcacggaa atgacctaga cgaacagcga gctgactgag
     1441 tcgctgttgt gaatgcgtcg aatcgcctcc ccaagtaggc gggcgatcga cagcacgcgg
     1501 attttcttgg aggcgcgcgc ctcctccgac atcgaaatgg agttggtgac gaccacctcc
     1561 tctagaggcg actcctcgat gcgctggacg gcgggaccgc tgagtacccc gtgcgtggca
     1621 caggccacta ctcgcctcgc cccgtgctcc atcaccgcgc gggccgcgtt gcagagggtg
     1681 cccgcggtgt cgatcatgtc gtcgacgatg atgcagtcgc gtccgcgcac atccccgatc
     1741 aggtgcatga cctcgctgac gttcggtcga tcccgacgtt tatcgataat cgcaagcatc
     1801 gcgcccagtc gcttcgaata agcgcgcgcg cgctccacgc cacccgcgtc cggcgagacg
     1861 aacaccgtct ccgcgttgaa gcgcggccgc aaatagtcgt ccaagaacac cggcagggcg
     1921 tgcagatggt cgaacgggat attgaagaat ccctgaatct gtcctgcatg caggtcgacg
     1981 cacaccacgc gggtcacacc cgcggcgacc agcagatccg ccaccagctt ggacgtgatt
     2041 ggcgtacggg gcgccacctt gcggtcttgt cgcgcgtacc cgtaatacgg aatcacggcg
     2101 gtgataccag ctgcggacgc gcgacggagg gcgtcgcaca tgatcagcag ctccatgatg
     2161 ttgtcgttca ccggtgagca cgtcggctga accacgtagg tgtcgagacc gcgcacattc
     2221 tcgcgaattt cgcagaaggt ctcgccatca ctgaagcggg ataccttcgc ctcgccgagg
     2281 ggcgtgtcga gaacacgagc gatctcggtt gcgagctccg ggttcgcgtt gccggtgaag
     2341 atgcaaactc tcttgagagc cacagtgcgc cccgtgtttc agcagccact atctgagtgg
     2401 ggtgagagcc tgcatggcct cacgcccagt tggcctcgga taaccgggcc tcgagcgctg
     2461 taacagctaa acgctcgtaa tgattaggaa tacgtaatcc agcggttttg cgcgcggtaa
     2521 ctagcagccg ctttgcgccc tgtcaacgcc cgtgaggccg gcaacgctgc acggatgagg
     2581 ggtatttggc tggccttgcg tggtgcggat cgcaaagcaa ggcaggcgta agtctcgact
     2641 accgataggg attttccggg aggtcgtctc cctcgccctt ctttttacgc gggcgcacgg
     2701 gaacgggcgc cgtcggggtc gccgcactgc gcgggcgggc tgggaccgcc gttgccgaag
     2761 gggccaccgc cgaaccggca cgcgccgcat acaccggagt ctcgaccgaa cccgtcggcg
     2821 ccggtgatgg agctggagcg gcgttggcgg aaggcgccgc aggcgcgacc gctgcgctcg
     2881 gacctggcga tgcggcgagc actcctgcct tcggctccgg cgcgcggaag aagcgtaccc
     2941 cgaagtaccc gccgatcccg ctgcccgcca gcaggaccgc gaacaacccc acccaggccc
     3001 accgcgaacg gcgcggcgcg tccggcacgg tgatctcacc gaggatgccg gtggcgtgcg
     3061 gcaagctaac cgccgccgcc gagacgctgg aggaaggcgg catcgacgaa acgtagcgcg
     3121 aagattgcgg aggaatcgac tccgtgcggg acagccagag gcgcacggcc tcccgctgac
     3181 gcgcgatctc atcgcccatc acttcttcga cgtagctcgc gagctcacgc gatgtagcga
     3241 tgcgggggcc gtaagcggca gcggtctcca acgcatccgc gaacgccgcg cacgtagaaa
     3301 agcggtggcg cggatcgcgc gacaaggctt gggcgatggc gtcgctgagc ggtctgccga
     3361 gctccggcac cgactcgagc agcggcggga ctcgctcggt catcactcgc gacaaggtcg
     3421 cggcctcggt atcggccttg aacagacgtt tgcccgtgag cgcttcccac agcacgatac
     3481 ccgctgcgaa gacgtccgcg cggcgatcga ggcgctcgtc gccgtgggcc tgctccggcg
     3541 ccatgtaggc gatcttgccc ttgagttgtc cgacacgcgt gccggacaag cgtgttgcgg
     3601 cacgggccac accgaaatcc gtgatgcgcg cgataccgtc cgagccaacc agcacgtttt
     3661 gcggggagac atcgcgatgc acgagttcga tcggcctgcc gtcgtcttcg tgcagctcgt
     3721 gcgcggcgtg aagcccgttc agcatgtcga gcgcgacccg tagcgtgatc gccgcgggca
     3781 cgcgccgccc tacgctcccg gcgcgggcga ggagacgcgc gagcgtgtcc ccctccacgt
     3841 attccatcac caagtaatag ccgaccagac tcgcgcccac ctcgaggatg ggcaccacgt
     3901 tcgggtggtg aatacgcgcg gccacccgcg cttcgtccaa gaacatctcc acgaactcgg
     3961 cctcgctggc caagtgagga tgcagccgtt tcatggccac gaaacgctgg aagccaccca
     4021 tccccgtgag ccgcgccaag tagacggtgg ccatgccgcc ggacgcaatc tcgccgatga
     4081 gttcgtagcg ttccacacgc tgccggccga gcgccggatc gaaaaggcga gcggctacca
     4141 cgcgactcag aagctccctt gggcgccgac aaaagcaccc tgacccaagc tcaacaccgg
     4201 tgtcacccgc gcgccgctcc ggggccgcgc cgcactcgca gagcgtctca cgcgtctacc
     4261 cgaaacgtac tcttccgatg gttcagagtc gggtcgactg ctccaatccg tccagaaaac
     4321 gccgacgaga gtggttgcga tcgacaggcc cgccgtgact ccgagcagca cgttggtgcg
     4381 acgttggcgg tccagcccat cctgataaac ggggcagctc gtgtcgccct gctcgcactc
     4441 gttcctgatc cggtctttgc cagggttctt gagcgtatcg attccgctga gggtggtcac
     4501 gagcacacca acgctagaca gcgcgactcc ggtgaaaaat acgccttgcg gcaaacccga
     4561 cgcggatcgg gccgcgtctt gcgctggctt cggcgtgggc gtcttcgcgg gctgatcgtc
     4621 tgccgacggg tccgtcgtct cgagtgatcc ctcgttcgtg tcctcccgca cctggctgtc
     4681 gaaggagaga tcgcccttcc cgcccgagcg ggccgtgact tcacgcgatt cgttacgatc
     4741 gccgccgtaa gcggcgcgca ccgattgcct gccttcgtac acatagatcc ggcgctgtaa
     4801 ctctggttcc cctggcacga tcttcccggc cacggcgagg tcgcaaggct ccgggcaagt
     4861 gacgaacagc tcgtacagcg actccctagc gcgcgccaaa atcccgggca ccagcttctg
     4921 gagggatgcc tcatccgaat agcggcgttc tgcgagcgca gccaaagtag cggcgcgatc
     4981 cagctggccc gccttatccc gcgctcgaat cgcgtactcc agcgcacgcg cgctcgggac
     5041 gctgtcatag gcatgctcga aatactcggc ggcctcgacc cagttctctt tcttgtatgc
     5101 ggcccgccct tgatcgaacg cgtttgcggc ctcgcgtatc tgcgcgggcg cgggttcgtg
     5161 agcggcgtgc gcgacctgtg gaacagcgca caaaccaagg ctcgagctca agagccataa
     5221 gcacggactc aacgaagcac gcagagagcg agtcaaagtt tcgggcagaa gactactccg
     5281 ttagcgctcg acgagcgagc ctcgtgtttc tgaaaatcag catgtttgcg cggatatgtc
     5341 cggtctcgaa tcggaactac caagctctgg acaggcatgt agggcaaggt ccgcaacatg
     5401 cgagttgcgg gcaacagaca tgcgacttgt gctgattatt gacgcccttg gacacgagcc
     5461 ctcaccgact tcgatgaaca cagcaaaaat acacgaacac tgatgtggtc cgctcgttct
     5521 cgcgcgagca agtgaggctg ccaacccacg gcgccgagtc ctcaccaacc ctccccagtg
     5581 atgctcgcag gcgccctagc gtctggcctc acgaactgca atcggttcca cccgctcaat
     5641 ccggctccgc catctccatg tgatgtcagc cccagctcga tggtgttgcc gtgctcggtg
     5701 cgacctcgcg cgtagccgcg ccgctttccc acaccgctcg tgcacaaaag acgaaggggc
     5761 ccgtgggggc cccttcacgc aaggcccctg aaactcacgg gccatcgagc tccaaccagc
     5821 tcagctcatc tcgacaatca ggcgatcggg ctcctgcaag tacgagatga tttcctgagc
     5881 gaaggccgcg cccacgtccc cgtcgatgat ccggtgatcg aacgagaaac tcagcatcat
     5941 cacgtctccg atcacgattt gatcgccgcg caccactggc ttccgcttga tggcgtggat
     6001 gcccatgatg ccgacttcgg ggtgattgat gatgggcggt gcgaggaagc cgccgagctt
     6061 gccgagcgaa ctgatcgtga agctcgagcc gccgagatct tctttctgga tcttgccgct
     6121 gcgcgcgagc tgcccgagac ggtcgatctc tcgggcgatc tcgaccaggc tcaaacgatc
     6181 tgcatcgcgg attaccggca ccatcaaacc ggcatccgtg gccgcggcga tgccgatgtc
     6241 gaagttcttg cgcagcacga gctcgtgtgc ggattcgtcc acgaccgcgt tcaggcgcgg
     6301 gtggcgcttg agcgctgcga ctaccgcctt cacgatgaac ggcaagaacg tgagcttgac
     6361 gttggaacgt tcggcgtgcg gcttcgcgcg gtcacgcaga gcaatcagcg gccctacatc
     6421 gcactcgtcg acgtagttga agtgggccgc cgtgtgcttc gagcgcgcca tgctctccca
     6481 gatgcgcttt cgcagaccgc ggatcggcat gcgctcgtcg gtctcgcgcg cagccgggcg
     6541 tggtgcctcg ggtagcggag cgcgagcggc gggggcttcg gatggcgcgg gctgagccgc
     6601 ggcaccactc gggcgctgcg cggctttctc cacgtcttct cgggtgaccc gaccagcacc
     6661 acccgaaggt tcgatgttgc gcaggtcaac gccgagctca cgcgcgagct tacgcgtcgc
     6721 gggagcggcg aggggcttgt cattgaagta gtcgttggcg cgcggggccg gggccgccgt
     6781 gggcttggcg gcgggcatcg cgctgattcc gggcagcgat tctttgatat cgccgactgc
     6841 cgtcgcgacg ggcgcagcct tcgcgggctc agccttggcg gccggctctg gtttcgcggc
     6901 tggctccggc ttggctgcag gggctccttc cccacccacc tcgaacacca ccagaacttc
     6961 gccgaccggg acggtgtcac cgaccttgaa gcgccgctcc gcgaccttgc cggccttggg
     7021 cgccccgatg gtcacggtgg ctttgtccgt catcacctcg accatgtcct ggttctcgga
     7081 gactgcgtcc ccttcttgaa ccaaccaatt gacgatctcg ccttcggcga cgccttcgcc
     7141 gatgtcggga agcttgaact cgaacttcga catgatcctc gtctcgtcgc tgtttcgatg
     7201 ccgcgacgag cgtcagaccc gcgcggcgtc caaaagagtg ggtaaaatgc ggtgcgagag
     7261 cggcatatac tccatctcga gcgtgtacgg gaacggcgtg tcgaaaccag ttacacgaac
     7321 gggtggcgct tcgaggtgat agaaagcccg ttcggtgacc aggctcacca gctctgcacc
     7381 aaagccgcag gtcttcggag cttcgtgcac aaccaccagc cggccggtgc gtttcacgct
     7441 ctcgatcacg gtgtcgatat ccacgggcca cagcgttctc aggtcgatca cctcggcttc
     7501 cacccctttg gcggcggcct tggcagcggc gtcgagcgcc tcgtacagca tggccccgta
     7561 cgcgagcacg gtgacgtgtt taccaggccg gaccaccgcc gcgctgccga tcggagtggt
     7621 gtaggccccg atcggaacgt cccccttggc ggcgcgataa acccgcttcg gttcgaagaa
     7681 gatgattgga tcgggatcgg cgatggaagc gagcagcaaa cccttcgcgt cgtacggatt
     7741 cgacgggcag acgaccttca gccccggcac gtgaatgaag gccgcctcgg ggtgctgcga
     7801 gtgatagtgc ccgccgcgaa tgccaccacc cacgggagta cggatcacga ccttggccgg
     7861 gtattcgttg cccgaacggt atcgatactt tgcgagttcg ttgacgattt ggtcataagc
     7921 cggccaaatg aaatccgcga actgtatctc tacgaccgga agcaggccgt agagcgccat
     7981 gcccaccgcg gagcccacga tgccgttctc gctcagagga gtgtcgatga cgcgatcgtc
     8041 tccgaactcg tcgaacagcc cggccgtgac gcgaaacacg ccgcccacct tgccgacgtc
     8101 ctcgcccaaa accactacgc gatcgtcatc acgcatcgcg atgcgtagcg cgtcgttgat
     8161 cgcctgaacc atgttcattt gcggcatgct gaccgtatac cgtgaaagtt aagagttacc
     8221 gtgcttggcg ggcgcgcgcg gcccactcag cacctcgttg cgttgctcga cgagatgcca
     8281 cggtggccgc tcgtatacgt cgtcgaacat cgactcgagc ggtggcggcg gcgttcgctc
     8341 ggcaactgca acggcttcgc gaaagcgctg atcgatgtct tggatcaagc gttcctctgc
     8401 agccccgtcc cacgcgtctc gtttttccaa ataggcgcgc acgcgaccga tcgggtcccg
     8461 cgaggtccaa ggctccagcg cctcactacc tcggtaacgg ttcggatcgt cgttggtgga
     8521 gtgaccaccc atgcggtagg taatggcctc aatcaacgtg ggcccctcac cgcgcacgcc
     8581 acgctccacg gcccggcgca ccgcggacac caccgcgaac aagtcgttgc catccacctg
     8641 cacgcccgcc acgccgtacg cggccgcttt ctcggccaag gtgcgcgtcg ccgactgacg
     8701 agccgtgggc acgctgatgg cccaaccgtt gttccgacac aagaacacgg tcgggatctt
     8761 gaacacccct gcgaagttca ttccgctgtg aaaatcgctc gagctcgtcg cgccgtcccc
     8821 gaagtaaacg agggtcgcta cgtcttgctt cttgattttg gcggcccacg cgaagccgac
     8881 ggcttgggtg atttgagtac ccacagggga gctgatcgat ccaaacccga cccgccggca
     8941 cgtgtagtga tccggcattt ggcggccgcg cactacgtcc ttggccgtgc cgaacatgtt
     9001 gtgaacgaac acctgcagct cgagcccccg catcagggca gcgccaagct cgcggtaaca
     9061 gggaaacaga aaatccgtct tccgcatcgc ataggccgag ccgatgatcg cggcctcttc
     9121 accgagcgag ccgacgtgaa accccactcg accttggcgt tgcagcgcga cgagccgctc
     9181 gtcgagcaag cgcgtggtca ccatgtgtcg atacatgtcg agcacctgct cggtcgacaa
     9241 gcccggatca tacgccggat cgagggtcag atcgtcattc agcacgcgca gcatgccgga
     9301 ctccggcggg gtggattcgg atcgctgtcg agtagtcgct accatgcgtt tggccttggt
     9361 tacctcaccg aaacaatacg tttttggcct cggcgacgct cgtctgcaag tcctgcgcag
     9421 gcaatccgag acgcttggtc agcgcccgca atagatcgag cttctccgtg cctagctcgc
     9481 cagcggcctc ggccaccccg aacagcgacg caacgcgcag gacctcgcgc gcctgctccg
     9541 gccgctctgc ggcgctcgcg atgctgtcga ggcgtcgctg cacgccctcg cgctgcaaac
     9601 gctcgtcgag catcgagaag agcccggata tctgcggttc tcgcaacagg ccgcctgaag
     9661 caaagaccac gatcttcttg aaagcctcac tctgccgcgg tagccgcgcc gcactacccg
     9721 caaccaacaa cgccgcctcc aagatggctt cgaacaagag agcagcatgc gggcagaagc
     9781 cggtgggctg cgtgaattca tcacactccg cgccataggc gtacgagctc gcagtggccg
     9841 cccagattga acgcgccgag gccccggcgg aggccgcttg ggaggagggc ggtaaaccca
     9901 gctgccgaac gactcgggtg atcaagtgca cgtcgagggc ctgcatgatc gccatttagc
     9961 ctaccaccac agcttcacga actcgaacgc acattgggcc cccgcttgga ggcggcgcgc
    10021 cgcaaaccgt ggcttggccc gtaattagcg ccgtaccagg ctgcgaacat cgatgatctg
    10081 ctcagcgctg ggcgcgctcc gcgccgctct ttcgagaaga gagggcggtg ccgcgagctc
    10141 cgaccccacg gccgccgcgg cttgcgtgag tcgttcggcc tctgcgtgcg ctcgacccac
    10201 gttcgaagcg gtaccagccg cagccgccag cggcttgccc agataagcgg caacgaagcc
    10261 ttcggcggcg tgataactgg aacgtaccag cggccctgac gcgacgtacg cgaagccaag
    10321 cgccctgccc tgtccggcat aggcgtcgaa ctgggcgggc tcgacatagc gctccacggg
    10381 ggcgtgcttc ggagtgggcc tcagatactg acccagcgtt acgatgtcga ccccggcgct
    10441 ccggagatcc gacattgctt cgtaaatctc atcgtccgtc tcaccgatgc cgaccatgat
    10501 cgagctcttc acgagccgct ccggggcagc ttgcttggcg tggcgcagca catcgatcga
    10561 gcgatcgtag ctgcagcgct gatcacgaat gcgcggggtg agccggcgag gtacctcgat
    10621 gttgtgcgcg aacacgtccg gtcggctccc gagcaccacg tcgatatccg ctcggcgtcc
    10681 gccgaagtca cccaccaggg tttcgacgag cagctctggc acccgctgct tcagctcgcg
    10741 caccgtctct gccacttgag acgcgccgcc atcgagcagg tcatcgcgat ccaccatggt
    10801 gaggaccacg taccgtagcc cgagtttctc gatcgcccgc gctacgtgca ccggctctcg
    10861 tggatcatag gctccccgcg gattgcccgt ggtcaccgca caaaaccggc acccgcgggt
    10921 acaggtgtgc ccgagcagca tgaccgtcgc ggtgccggaa gcccagcact ccccaacgtt
    10981 ggggcagcga gcttcttcgc acacggtgta aagatcgagc ccgcgaagcg tctccttcag
    11041 gcgcgtgtaa ttgtcgccac cgggagcccg aaccttcaac caacccggct tgggttcctt
    11101 gaacgcgggc gccttcgcgg ctgacgcagg cccagaaagc gactttgctc gcgtgcccga
    11161 cgctgcggcg tcaccccgct cgcagagatc gccctcctct gaggggccat gctcggacac
    11221 gctacgctct gccactaaga gcctcctaga atcaaactct cgagcagctc attgacgagc
    11281 ttcgggttgg ccgatccgcc tgtctccttc atcgcctgac ccacgaagaa gccgagcacg
    11341 cccttctttc cgctacgcag gctctccgcc tgactgggga atttctcgat cagtgcttgg
    11401 caaacgcgac gcaggctgtc cacgtcggac actaccttca tgccgcgttc ttcgacaatc
    11461 gagcttggca tgcgtgtcgt gttctcgatg gcggaaaaca cttctttggc ctgcttgccg
    11521 gagatgtccc ccttctcgac cagggccagg agctctgccg tttgctccgg cgtgaccgtg
    11581 aaggtggctt cgagcccgcg cgtcttcgct ccgcgcagca cgtcattggc gaagaagttc
    11641 gcggccttca ccggattcgg gaacaccgcg cagagctggt cgaagaagcg cacgtagccg
    11701 gggtgctggc tcatcgtcgc ggcggcgggc gcggcaatgc cctgcgagag ccagcggttg
    11761 cgcacggcgg tgggcagctc cggcaaggac gactcgcact tgcgtacgaa cacgtcatcc
    11821 agcacgagcg ggcgcaaatc cggctcgggg aagtagcggt agtcgtgcgc ctcctctttg
    11881 gagcgcaatg tcttggtgag cccggtgtcg ggatcgaaac tacgcgtttc ctgagtgacc
    11941 ttctgcccgc tctcgagcaa cgccgtctgc cgcgcgacct cggagtcgat ggcgcgctgg
    12001 acgaagcg
//
LOCUS       DASTKU010000510         7340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1658922, whole genome shotgun sequence.
ACCESSION   DASTKU010000510 DASTKU010000000
VERSION     DASTKU010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658922"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1775)
                     /locus_tag="VFQ61_17560"
     CDS             complement(180..1775)
                     /locus_tag="VFQ61_17560"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET9956322.1"
                     /translation="MGNYLGCAARWALVCLALGGGAGCAAAEDGESPGARASAVVYGS
                     DDRVPYSAVTDARLRGWADATAALFIDENVSCAEAACSLTTRPIEAVTGALCDGQPLQ
                     DQAVGAACTAFLIAPDVMVTAGHCLEDCRFGEFPAREVRFVFGFQTDTRGEVPREVPA
                     ENVYRCAEMLGRDMTGMSEADRDFAVFRLDRPVTGRTPLVPAPAGTRVSEDASLVILG
                     HPRGVPLMVAAGGRVTANDATLPRFYSSLDHLPGNSGSPVLDAATGLVHGILVTTPAT
                     AEFDTVFTPTGDLCLEERVCDEALGCGGYLPSSSWAGVMRIGAASACRDGQKDGLESD
                     VDCGGAPECVRCAEGRACSADLDCTSGRCAAGVCAVPYCEDGVRDHGESDVDCGAGCA
                     TACAVGLRCSEHLDCQSNSCVHGRCAQPSCVDGRYNGDETDVDCGGPCKPCRVGQGCS
                     ADSECRIGVCDFGTCIAPTCPDGVLDGAETGVDCGGGCFPCAEGVVCTTNDDCSSGSC
                     KQGRCVKRPPVTCHSAKPRSRRL"
     gene            complement(1883..3208)
                     /locus_tag="VFQ61_17565"
     CDS             complement(1883..3208)
                     /locus_tag="VFQ61_17565"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HET9956323.1"
                     /translation="MTGVYRTLGAVVAAAAALASITHTTDANALGRKEYVVVLLDRSG
                     SMLSDAYFDGTTHRSKWKEAVQQAISRVEGQRDNKDRRPSSDSTWEDPIYTPPATSDF
                     YPESSHCYQIWTFEDTSYTVRFPTDGTWFCAAGADGFTKDKSKYATVINQINTVAAPA
                     SAGPTTPLAHSLCQALTIVRDAAQSDPLYGDPNNVQIRRSIIMQSDGLENSTPSTDQC
                     YGSVPNTATFNPTLASAAHPGFYEWAGGLAVNSWERKVYNMAFLSDPAEPPFPWTVGG
                     NQYWGLKPDWNFLTFYQTVYNYPEAIAIDASGLVSLNIDALFTYIPTAAAKAAATPDV
                     DPQLQALFRNYSKSTGGRYQRIQYDGTKAALLGTAHNPPADVSDDGCVNNADYSMITQ
                     SDVYGKRDSEGQIVKRADVTRDGWVNSKDRDLVMRTWGQKRPGFQGTCP"
     gene            complement(3517..6159)
                     /locus_tag="VFQ61_17570"
     CDS             complement(3517..6159)
                     /locus_tag="VFQ61_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956324.1"
                     /translation="MTAACADGDPVADRRSASGGAGGGTSGEGSAAGSLTGGVSVGGA
                     PESGGTAQAGEAGALGGSSSGEVGQGGEPTAGFGGCNSVAGCGGSGGHGGSGGRGGSG
                     GNAGSHSPGGESSGSGGATAGETGEGGETQGPSPSCVDGDPCTLDLWNGSTCEHPAAP
                     ESTPCDDGRFCTSDDQCVSGVCVGSGFSSEAAVLGEVRAFGGFAESRRDNGLVALLSE
                     RRAIFADESGLGTELTLVGVESGKVIPLSTARSNMRLARAPISSWLWVNQLTNHIVPL
                     SSDRFAFLGSRQIEIYDVLDEQMVRRSIGAIPERIGAAAGTVFAAAGWDGRLWVGGDL
                     GLSELTVSEAGEVTLGPEQPKINHCHGLALSPDGKTLYVATLRGLHVMAVGEDGALTE
                     SAYLYDGDALFNVRVNESWLAIQKMVPPVGFGDTDVLRVSDLSRLATFPYVSGKPIPL
                     GFALTDTGILVERYEFRGADGEGFTAEHYGFSAGTLVLQSSTEYRDRQSGEVSIHRDL
                     ARPEALGGKVVLTPLRQVFDLTRAGFTPALAERQGSFETVRALDATRVAAIGTYTSQT
                     IDLSTPLEPAVRGRGVLSPAVQPLKLELNEQSGRLRMLSAYPDEDILRLPLNDARDEV
                     SVFSPDTKGVLAFAGSFELDRGPSHLFAASGSLFQLSAMGRDQFVLRRFGWDRVASAS
                     GGAALVADVEASLAVPADDTHDHFVWRFHVDEATGDAVIVDSQQDVGTGQTSTSLTWA
                     TLGRDGFTDAVSRVHRTGDSFSFVLDVKVFGDRAAVLSNEFLSVVKRHGDELEIIAEL
                     NHGVSSTLNPSTLLGFDGDAIYLGDGLDAGSASVRVHVLDSNDLTERASYELPEPVVG
                     MASAGSTIAFASANRVFTASPACE"
     gene            complement(6252..6893)
                     /locus_tag="VFQ61_17575"
     CDS             complement(6252..6893)
                     /locus_tag="VFQ61_17575"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9956325.1"
                     /translation="MSAISQRIVSARILVTGETRALVEAWYAELEAVGAPCVAAHGVA
                     EARRVLSSERIRLAIVEAGAEPGRFDPWPENQRRPRVLFLMTAFDADVAVGLARAGDL
                     VLPAALGMGTLPKAVRMLCSPDDEVVDFSRKYRLSPRETELLRLALLGKNNDEAAAEL
                     NCGRGTVSTFWNRIFKKTKVSGQRDVIVLLLRSSQGDTLLEEPLSLSRSSANH"
BASE COUNT         1463 a         2487 c         2085 g         1305 t
ORIGIN      
        1 aggttttcga atcccgtcat cccagcaccc aaatcagcaa tctcgaaggt agaattgacc
       61 cgacgctcgc gcgatgagcg cgcccgtacg aatcccgaga atccgcacac tcgcctgctc
      121 cactcacctt acggagccaa cttacacgat gccgagtgac agcatcatat gtgttttgat
      181 tacaagcgtc tcgaacgagg tttcgccgaa tgacaggtca ccggcggcct ctttacgcag
      241 cgaccctgct tgcagctgcc gctcgagcaa tcgtcgttgg ttgtgcagac gaccccctcc
      301 gcgcacggaa agcagcctcc gccacagtcc actcccgtct ccgccccgtc caggacgcca
      361 tccgggcaag ttggggcgat acacgtaccg aagtcacaga ctccaatccg gcattcggag
      421 tcagctgaac acccttgccc tacccggcac ggcttgcagg gtccaccgca gtcgacatcc
      481 gtttcgtcgc cgttgtagcg gccgtcgacg cacgacggct gagcgcagcg gccgtggacg
      541 cagctgttac tctgacaatc caggtgctcg gaacagcgca gacccaccgc gcaggccgtc
      601 gcgcagcccg cgccgcaatc aacgtccgat tcgccgtgat ctcgcacgcc atcctcgcag
      661 tacggcaccg cacaaactcc ggcagcgcag cggccactcg tgcagtccag atccgcgctg
      721 cacgcgcggc cctcggcgca ccggacgcat tctggtgcgc caccgcaatc cacgtccgac
      781 tcgagcccat ctttctgccc gtcacggcaa gccgatgcgg ccccgatgcg catgacgccg
      841 gcccacgagc tcgagggcag atatccacca cagcccaagg cttcgtcaca gacgcgctct
      901 tctaggcaca ggtcgccggt gggcgtaaac acggtgtcga actctgcagt cgcgggggtg
      961 gtgaccaaaa tgccgtgcac gaggccagtc gcggcatcca gaactggact cccgctgttg
     1021 ccaggcaggt gatccaggct cgaatagaac cgcggtagcg tcgcgtcatt ggcggtcacc
     1081 cgcccgcccg cggcgaccat cagcggaacc ccgcgcggat gtccgagaat gacgagtgag
     1141 gcgtcttccg aaacacgcgt ccccgccggc gcgggaacca gcggcgtgcg tccggtcacg
     1201 gggcgatcga gccgaaacac tgcgaagtct cggtcggctt cggacatgcc cgtcatgtct
     1261 cgacccaaca tctccgcgca tcgatagacg ttctctgccg ggacttctcg cgggacctcc
     1321 ccgcgagtgt cggtctggaa accgaaaacg aagcgcacct cgcgtgctgg aaactctccg
     1381 aatcgacaat cctccaggca gtgccccgcc gtgaccatca cgtcgggagc gatcagaaaa
     1441 gccgtgcacg ccgcaccgac cgcctgatcc tggagaggct gcccgtcgca aagcgctccc
     1501 gtaaccgcct cgatggggcg tgtcgtgagc gagcaagcgg cttcggcaca gcttacattc
     1561 tcgtctatga acagagccgc ggtcgcgtca gcccagccgc gcaagcgcgc atcggtgacc
     1621 gcactgtacg gcacgcgatc gtcgctccca taaacaaccg cagatgccct ggcacctggc
     1681 gactcgccgt cttcggcggc cgcacaacct gccccgccgc ccaaagccaa acacaccagc
     1741 gcccagcgcg cggcgcagcc cagataattg cccatgaatg cgactccgcc tcaccagcgt
     1801 aaggactcgc gcccgcccga gactaccctc gagcggacgc gagctaacaa ctcaatatct
     1861 cagaatcgag tggtaacgct tctcacgggc aggtgccctg gaacccgggg cgcttctggc
     1921 cccaagtgcg cattaccagg tcgcggtcct tcgaatttac ccaaccatcg cgcgtcacgt
     1981 cggcgcgctt cacgatctgg ccttccgaat cgcgctttcc gtacacgtca ctctgggtga
     2041 tcatggagta gtccgcgttg ttcacgcaac cgtcgtcgct gacgtccgcc ggcggattgt
     2101 gcgccgtgcc gagcagcgct gctttcgtgc cgtcgtactg aatgcgctgg tagcgaccac
     2161 ccgtagactt cgagtagttg cggaacagcg cctggagctg cggatcgacg tcgggcgtag
     2221 cggctgcctt ggcggccgcc gtggggatat aggtgaagag cgcgtcgatg ttgagggaca
     2281 ccagaccgga ggcatcgatc gcaatcgctt cggggtagtt gtaaaccgtc tggtagaacg
     2341 tcaggaagtt ccagtccggt ttgagccccc aatactgatt gccaccgacc gtccagggga
     2401 atggcggctc cgcggggtcc gagaggaacg ccatgttgta gaccttgcgt tcccagctgt
     2461 tcacggcgag gccgcctgcc cactcgtaga aaccggggtg agccgcgctc gccaaagtgg
     2521 ggttgaatgt cgcagtattc ggaacggagc cgtagcattg gtccgtcgac ggggtgctgt
     2581 tctcgagccc gtcgctctgc atgatgatgc tgcgccggat ctggacgttg ttgggatctc
     2641 cgtaaagagg atccgactgc gcggcgtcac gaacgatggt gagcgcttgg caaaggctgt
     2701 gggcaagcgg agtcgtggga ccggcgctcg caggcgcggc aaccgtattg atctggttga
     2761 tgacggttgc gtacttgctc ttgtccttcg tgaagccgtc ggcgccagca gcgcagaacc
     2821 aggtgccatc cgtggggaag cggacggtgt aagacgtatc ttcgaacgtc cagatctgat
     2881 agcagtggct gctctcggga tagaagtcgc tcgtggcagg cggagtatag ataggatcct
     2941 cccaagtcga gtcactggaa ggacgtcgat ccttgttgtc ccgctgaccc tcgactcgcg
     3001 agatcgcctg ctgcacggct tctttccact tgctgcgatg ggtcgtgcca tcgaagtaag
     3061 cgtccgacaa catgctcccg cttcggtcga ggagaacgac tacgtactcc ttgcggccaa
     3121 gcgcattggc atccgtcgta tgagtaatgc ttgccagcgc cgccgctgcg gcgaccacag
     3181 cccccaacgt tcgatacacg cccgtcatga ttcacaccta tgctgacgag gagacacccg
     3241 cacgagaccc actcccgtta ccggtgaccc accaacttcg tcgggaataa agtactcgat
     3301 caatcgttat tcacaaagcc gaatcgcacc gcgctctaca cagagtgcct atgtcattcc
     3361 aaactgacaa tcacgcgcga gcccatgact cagccgattg ctagtccggg tagcgggctc
     3421 gcgcggccag agctgcttcg acaacggcgc tgtcatcatt gaatgacagc gctcgcggct
     3481 ccagattgcc tttccctggg cgtaaagagc cgaccgtcac tcgcacgcgg gcgacgccgt
     3541 gaagacccga ttcgcgctcg cgaatgcgat cgtgctccca gcgcttgcca ttccgaccac
     3601 tggttccggc agttcgtacg atgcgcgctc ggtcagatcg ttcgagtcga gcacgtggac
     3661 tcgcacgctc gctgagcctg catccagacc gtcgcccagg tagatggcat cgccgtcgaa
     3721 accgagtagc gtactgggat tcagagtgct cgaaacgccg tggttcagct cggcgatgat
     3781 ctcgagctcg tcaccgtgcc gcttgaccac gctcaggaac tcgtttgaga gtacggccgc
     3841 ccgatcgccg aacaccttca cgtccaagac aaagctaaag gagtcgccgg tccgatgcac
     3901 tcgcgacacg gcatccgtga atccgtcccg gcctagggtt gcccaagtca ggctcgtgct
     3961 cgtttgaccc gtcccaacat cttgttgact gtcgacgatc acggcgtcgc ccgtcgcctc
     4021 atccacatga aaacgccaga caaagtggtc gtgagtgtca tcggcgggca cggccaagct
     4081 cgcctcgacg tccgcgacga gcgcggcccc gccgctcgcg gaagctacgc gatcccagcc
     4141 gaagcggcgg agcacgaatt ggtccctgcc cattgcgctc agctgaaaca ggctgcctga
     4201 cgcagcaaac agatgcgacg ggccccggtc taactcgaac gagccagcga aggcgagcac
     4261 gccctttgtg tccggggaga acaccgaaac ttcatctctc gcatcgttca acggcagccg
     4321 gagaatgtcc tcgtccggat atgcactcag catgcgcagc cgccccgact gctcgttcaa
     4381 ctctagtttg agcggctgga ctgccggaga aagcacgccc ctgccccgta ctgcgggttc
     4441 gagaggcgtc gacaagtcga tggtctggga ggtgtaagta ccgatcgcgg caacgcgggt
     4501 cgcgtcgagc gctcgtaccg tctcgaaaga accttgccgc tctgcaagtg cgggggtgaa
     4561 accagcacgc gttaggtcga atacttggcg cagcggagtg agcaccacct ttccacccaa
     4621 cgcttcgggg cgcgccagat cgcggtgaat gctcacctcg ccgctttgcc ggtcccgata
     4681 ctcggtcgaa ctctgaagga cgagcgttcc cgcagagaat ccatagtgtt ccgccgtgaa
     4741 gccctctccg tcggccccgc ggaactcata gcgctctacc aggatgccag tatccgtcag
     4801 cgcgaatccg agcgggatcg gtttccccga cacataggga aacgtcgcca accgggaaag
     4861 gtcggatacc cgcaagacgt cggtatctcc aaagccaacc gggggcacca tcttctgaat
     4921 ggcaagccag gattcgttca cgcgaacgtt gaaaagagca tcaccgtcgt acagataggc
     4981 cgattcggtg agagctccat cttcacccac agccatgacg tgaagaccgc ggagcgtggc
     5041 cacatacaat gtcttgccgt caggcgacag ggcgagccca tgacagtgat tgatctttgg
     5101 ttgttcgggt cccagtgtga cctcgcccgc ttccgaaacg gtcagttccg agaggcctag
     5161 gtcgccccca acccacaaac gcccgtccca ccccgcggca gcaaacactg tgccggctgc
     5221 tgcaccaatg cgctcaggga tggcgcctat cgagcgacgt accatctgct cgtcgagcac
     5281 atcgtagatt tcgatttgcc tcgagcctag aaaagcaaat cggtcgctcg agagcggcac
     5341 gatgtggttc gttagctgat tgacccaaag ccaagagctg atcggtgcgc gagcaagacg
     5401 catgttggaa cgcgcggtcg aaagcggaat caccttgccg gactccactc ccaccagcgt
     5461 gagttctgtc ccgaggccgg actcatccgc aaaaatggcg cggcgctccg aaagcagggc
     5521 caccagcccg ttatccctcc tcgattcagc aaatccgcca aacgctctca cttcaccgag
     5581 cacagccgct tcactcgaaa agccgctccc gacgcacacg cccgatacgc actgatcatc
     5641 cgacgtgcag aaccgcccat catcgcaagg cgtcgactct ggggcggcgg gatgctcaca
     5701 ggtgctcccg ttccacagat cgagcgtgca tgggtcccca tcgacgcaag acggactcgg
     5761 cccctgcgtt tcacccccct ctcctgtttc tcccgccgta gcgccaccgg aacccgaaga
     5821 ctcacccccc ggcgagtggc tccctgcatt gccgccggaa ccaccgcgac caccagaacc
     5881 gccgtggccg ccagaccctc cgcaccccgc aacagagttg caaccgccga accccgccgt
     5941 gggttcacca ccttgcccaa cttcaccgga ggagctcccg cctaacgcgc cggcttcacc
     6001 cgcttgtgcg gttccgccgc tctcaggggc accgccaacg gaaacacctc ccgtaagcga
     6061 acccgccgct gagccttcgc cgctcgtgcc gccacctgct ccgcccgacg ccgaccgtcg
     6121 gtcggcaacc gggtcaccgt ccgcacaggc ggcagtcatg agaaccagaa gaatcccgcg
     6181 caccctcggc atgcgcgcgg aaggtcgcac ccgggcaccg ctttcgcaag acaaaagcat
     6241 agaaggatgc ttcagtgatt ggcgctcgac cgcgacaaac tcaacggctc ctccagcagc
     6301 gtgtcccctt gcgaggaacg aagcagaaga acgataacgt cgcgttgccc gctcaccttc
     6361 gtcttcttga agatgcggtt ccaaaaggta gagacggtgc cgcggccaca gttgagctcc
     6421 gcggcggcct catcgttgtt cttgcccaag agtgcgagcc gcaagagctc cgtctctcgc
     6481 gggctcagcc gatacttgcg ggaaaaatca acgacctcgt cgtccggcga acacagcatc
     6541 cgcacggctt tgggtaaggt tcccatgcct aacgcggccg gcagcacgag gtcccccgcg
     6601 cgcgccaaac cgaccgccac atcggcgtcg aacgccgtca tcaagaacag caccctaggt
     6661 cggcgttgat tctccggcca agggtcgaat cgccctggct ctgcacccgc ttcgacgatc
     6721 gcgaggcgta tgcgttctga tgacagaacc cgccgtgcct ccgcgactcc atgcgccgcg
     6781 acgcacggag ctccgacggc ctctagctcc gcataccaag cttcgacgag agcccgcgtc
     6841 tctcccgtaa cgagtattcg ggccgaaaca atgcgctgtg aaatcgcact catacgactg
     6901 aaccaccctt gctcccatcc aacacgagac ccacacgtct caatgcttgg acacccgacg
     6961 tttcaactca aactccaact ctgcgtagct ctgcctgatc ctgcctctga ccactccaac
     7021 tctaacctcg cacggttata cagttcgaag tgatctgtca actcccaggg cttgctcgag
     7081 ctctttgctt tggaggccca cagtgttaac gccggtcagt gttaatcgcc gggcaatctt
     7141 aacgccggtc agtgttaatc gccgggcaat cacacgggcg gcaatcacac gggcgacagg
     7201 tcagtctagt tcagctctga tcgaagcggc cacttgatct cccgcaaaag cctgctttct
     7261 cagctcgttc aagcagttcg tagctcgagc tgtgtttgct ctgctggaag ttctggatga
     7321 caccattttg accatcgcgt
//
LOCUS       DASTKU010000511         4891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_320202, whole genome shotgun sequence.
ACCESSION   DASTKU010000511 DASTKU010000000
VERSION     DASTKU010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320202"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            996..1922
                     /locus_tag="VFQ61_17580"
     CDS             996..1922
                     /locus_tag="VFQ61_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956326.1"
                     /translation="MGQVKAGKQKTQRVQVEVGPSEATPRQLSRGARSRSSVPVQRVI
                     PVKPRSAVIESVSVNSGEIHAAAAFQRVLPELQDLSKRSLIPVNLNVASVVARVLALI
                     PGLRPLRPELERSLLDFRGDWFDRLEDYALALHHAQSQYLAHAQPKPPRELTANSIGL
                     RALLLAEVDLLIRRGSLDEQVVERVRGFVGYTNLATDLRTLAQLIETHRAKIEGHSPL
                     ALADAERARALAAKLLEAQRARAAASAELARVRAVRDRAFSLLVRAYKETRAGVLYVR
                     RNHANVAELVPSLFSDRGDRGARPRPVDRYLG"
     gene            2034..3020
                     /locus_tag="VFQ61_17585"
     CDS             2034..3020
                     /locus_tag="VFQ61_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956327.1"
                     /translation="MESTANTLGATTRVDAVGQEQAAIEAVERLKPRYQALIPEELIQ
                     VNLDVPAAIVTVLGALPAIRNLRPSVVEQLPKHDIGLFDSLEDAALALNELHVQFLIA
                     TRPTDDLEQLLSEATQLRDLLLSDVTALTKRGLIEGNHLDELKGPIGYKNLAVDLNIL
                     YVALRSSWAKIQGKSAVTEAELDRAGKLQQRMMRVVGFRELGASAMATSNDLRQRAFT
                     YFMRAYDDARRVVTYLRWEEDDADTIAPSLYSGRGTGRRKNAEAAGSPGNPASGATTA
                     SPTQPSGAAGAGSAAGQAGAAATSPAGNAGASTSTASTSGGSGRPGDDPFMH"
     gene            3110..4600
                     /locus_tag="VFQ61_17590"
     CDS             3110..4600
                     /locus_tag="VFQ61_17590"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HET9956328.1"
                     /translation="MAKPPTLNLGAWRHIDGEAGGPVLRLPAHHLVTHGVVVGMTGSG
                     KTGLVTVLAEEALGAGIPTLIIDVKGDLPNLLLAFPDFEPTAYLPWIEPGVQGQSSPS
                     PDEQATALARERRSALEAWSIGERELKRFSSRTSLRVLTPGATAGEPLHVLSPLERRS
                     ERWDSDPESARAALSAAVSLVLRLLGRDPDPAKSKEHVLLSVLAERRLVAGEQADLAA
                     LMQDVAEPPISEIGALSIDAFMRPRDRNNLAAALNTLLASPTFASWRVGANLDVADWL
                     TPRGRRTPAVIVSVAHLDDEERNLVLGVLLEEVLSWVRSLRGSQTLRALLIFDEVYGF
                     LPPHPANPPTKRPLVALMKQARAFGVGVVVATQNPMDLDYRALSNAGLWCIGRLQTDA
                     DRARVIEGLATAQDIHEANSAELSRTITRLNNRWFVVRNSHANSGPILLQPRYALSWL
                     RGPMTRHELRLAREHREQVEGNAPASTPPEVAPAVTAEGHRESETR"
     gene            4641..>4891
                     /locus_tag="VFQ61_17595"
     CDS             4641..>4891
                     /locus_tag="VFQ61_17595"
                     /inference="COORDINATES: protein motif:HMM:NF019057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HET9956329.1"
                     /translation="MAHPRFMISKSPGGQFTFNLTAANGAVILASERYTSHSAAVAGA
                     RSVQAHAGDDGNYEHRTASDGRHYFVLIAGNHEIIGTSQ"
BASE COUNT          922 a         1525 c         1596 g          848 t
ORIGIN      
        1 gcgtccggag attgggaaac acgatgagag gctgattgct cgcgaactgg cttcaaatca
       61 gccgaaacac cgagcggacc gaaagaatgg ttcaacgcgc aatagccaat cagcggccgg
      121 ccgtagatcg gtctgatacc gaatctcaga atctgcaccc gcgagctcga gagaccagaa
      181 tctcaaatca tttctggcac ccacgagccg actcggccgt tgcggggagt cggaaagggc
      241 agccgggagc ctatttcgat agggggtcga cccaaaggtg acggtcgacc cgtcactttt
      301 cggtcggaga agatgacagg gtgacggtcg acccgtcttt ctcgatggag ccgaatgggc
      361 tcggtgaggg gtgaaccgtc agatggcggt gagcactcaa ttcatcttcg ccaccgaccc
      421 gtcgccaggg ccgcatcccg gccgaagcgt gacggtgggc cagtgctgac ccgaaagagc
      481 ccagcaagct gagctgggcg caccgtctct aggcattggg cggcgcctcc tcgagggctg
      541 gggagcgcat ggagcgagtt cggatgctct ctctcccgag gatgagacac tgcttgcatc
      601 ccgcgccgtt cagcagagtg ggtctcgact gaggagcacg taatgggctg tttgaataaa
      661 agcccgcact agccacgctg ctaatgcaag gggctttgcg gaggagtgct cggctgctgt
      721 caccgtgcat cgagcgtggg tttgttgatc tccgtgtagc caagagattg gctggccccg
      781 tgtcacaggc ccagcctgta cgcactgtgg aactggttct ctgtggcctg cggttgagct
      841 gggtcaccag attcatgacg cgataaatag tagttcgggg gttgcgtagg agagggcagc
      901 tgtttagagt ggtctcggtt gggaatacgc cgctgggttg aggcgtatgc tgaatgggcg
      961 gtcacggggt ggccgcaaca tgggacggtt tgccaatggg gcaagtgaaa gcgggaaagc
     1021 agaagacgca aagggtccag gtcgaggtcg ggccgtcgga ggcgactcct cggcagcttt
     1081 cgcgcggggc gcgctcgcgc tcgagcgtgc cggttcagcg ggtgatcccg gtgaagccgc
     1141 gcagtgcggt tatcgaatct gtgtccgtga actcggggga gatccacgcc gcggcggcgt
     1201 tccagcgcgt gcttccagaa cttcaggatt tgtccaagcg atctttgata ccggtcaatc
     1261 tgaatgtggc ttcggtggtg gcccgggttc tggcgctcat tccagggctc cggccgctcc
     1321 gtccggagct cgagcgctcg ctcttggatt tcaggggcga ttggttcgac cgcttggagg
     1381 attacgcgct ggcgctgcac catgcccaga gtcagtatct ggcgcacgcg cagccgaagc
     1441 caccccgtga attgacggcg aacagtatcg gactgagggc gctgctgctc gcggaagtcg
     1501 acctgttgat tcggcgcggc tccctggatg agcaagtggt ggagcgcgtg cggggcttcg
     1561 tgggttacac gaacctggcg accgatctgc gcacgctagc ccagctgatc gaaacgcatc
     1621 gggcgaagat cgaagggcac tctccgttgg ctttagcgga cgcggagcgt gctcgggcgc
     1681 tcgcagcgaa gctcctcgaa gctcagcgag cgcgagcggc ggcttctgcc gaactcgcgc
     1741 gcgtgagagc ggtacgcgat cgggcgttct ccctgctcgt gcgggcttac aaggagacgc
     1801 gcgcgggggt gttgtacgtg cggcgcaatc acgcgaatgt cgcggagctg gtgccatccc
     1861 tcttttctga tcgcggcgat cggggtgcac gcccgagacc ggtcgatcgt tatctcggtt
     1921 gaagttcgcg ggaaggttca tccccgcatt ccgtaaacac aatgtgagtt caggggcgct
     1981 cgggaattcg agcgttcggg gtccgtttac atcaagaaag gtttggggca atcatggaaa
     2041 gtacagcgaa tactttgggt gcgaccacac gtgtggacgc ggtcggtcaa gaacaggcgg
     2101 cgattgaagc cgtggagcgg cttaagccgc ggtatcaggc gctcattccg gaagagctca
     2161 ttcaagtcaa cttggatgtg cccgcggcga tcgtgaccgt gctgggtgca ttaccggcaa
     2221 tccgtaactt gcgcccgagt gtggtcgaac agttaccaaa acacgatatt ggcctattcg
     2281 atagcctgga agacgccgcg ttggccctga atgagctgca tgtgcagttt ctgatcgcca
     2341 cgcgcccgac cgacgacctc gagcagctgc tcagtgaagc cacccagctt cgcgacctgc
     2401 tgctctcgga cgtgacggcg ctcaccaagc gcgggctcat tgagggcaat cacttggatg
     2461 agctgaaggg gcccattggc tacaagaacc tggccgtaga tctgaacatt ctatatgtcg
     2521 cgttgcgcag cagttgggcc aagattcaag gcaagtctgc agtgacggag gccgagctcg
     2581 accgggctgg caagctgcaa cagcgaatga tgcgcgtggt gggcttccga gaactcggcg
     2641 cgtccgccat ggcgaccagc aatgatttac ggcagcgggc attcacgtac ttcatgcggg
     2701 cttatgatga cgcgcggcgc gtggtgacgt acttgcgctg ggaagaggac gacgcggaca
     2761 ccatcgctcc gtcgctctac tccgggcggg gcaccggaag gcgcaagaac gcggaagcag
     2821 ccggttcccc cggaaacccg gcttccggcg ccaccacggc gagcccgacg cagccctctg
     2881 gcgccgccgg agccggcagc gcggcgggcc aggcaggggc cgcagcaacc agcccagccg
     2941 gcaatgccgg cgcatccacg agtaccgcga gcacgagcgg tggttcgggc cgtccgggag
     3001 atgacccgtt catgcattga gagcgcgtga cacgcacccc agggcactcg cgaaagtgcc
     3061 ctggggtcgg gcaattcggc aactcgaacg ttcgggaaac gaaagcatca tggcgaagcc
     3121 acccaccttg aacctcggag cctggcgcca catcgacggc gaagcaggcg gccccgtgtt
     3181 gcgcttgcca gcgcaccacc tcgtgaccca cggcgtggtg gtgggcatga ctggcagcgg
     3241 caaaactggc ctcgtcaccg tcctcgcgga agaggcgctc ggcgcgggca ttcccaccct
     3301 catcatcgac gtgaaggggg acctacccaa tctgctgctc gcgtttcccg acttcgaacc
     3361 gaccgcctat ctcccctgga tcgagccagg ggtgcagggg cagagctcac cgagcccgga
     3421 tgagcaggct acagctctcg cgcgcgagcg gcgcagtgcc ctcgaggcgt ggtcgatcgg
     3481 agagcgcgag ttgaaacgct tcagctcccg caccagcctg cgcgtgctca cgccgggcgc
     3541 caccgcgggg gagcccctgc atgttctctc gcccctcgaa cggcgctccg agcgctggga
     3601 cagcgaccca gagtccgccc gtgcggcgct cagcgccgcc gtgtctctcg tcttgcggct
     3661 cctcggtcgc gacccggatc ccgccaagag caaagagcac gtcctgctca gcgtgctcgc
     3721 cgagcggcgc ttggtggcgg gcgagcaagc ggatctagcg gcgctcatgc aagacgtcgc
     3781 cgagccaccc atcagcgaga ttggagccct ctcgatcgac gccttcatga ggccgcgaga
     3841 tcggaacaac ctcgccgccg cgctcaacac cctcctcgcg tcccccacct ttgcgagctg
     3901 gcgcgtgggc gcgaacctcg acgtcgcaga ctggctcacc ccgcgcggcc gccgcacccc
     3961 cgctgtcatc gtcagcgtgg cgcacctcga tgacgaagag cgaaacctcg tgctcggagt
     4021 gctgcttgaa gaagtgctct cctgggtgcg cagcttacgc ggctcacaaa cactccgtgc
     4081 cctcctgatc ttcgacgaag tgtacggctt tttaccgccg cacccggcca acccgcccac
     4141 caagcgcccc ctcgtcgcgc tcatgaaaca agcgcgcgcc ttcggcgtgg gcgtcgtcgt
     4201 tgccacccag aaccccatgg acctcgacta ccgggcgctc tccaacgcgg gcctctggtg
     4261 cattgggcgc ctccagaccg acgccgatcg cgcgcgcgtc atcgaaggct tagccaccgc
     4321 ccaagacatc cacgaagcca actccgccga actcagccgc accatcaccc gcctcaacaa
     4381 ccgttggttc gtggtgcgca acagccacgc gaactccggc ccgatcttgc tccagccccg
     4441 ctacgccctc tcgtggctac gcggcccgat gacacggcat gaactccgcc tcgcgcggga
     4501 gcatcgcgag caagtcgaag gcaacgcacc ggcttcaaca ccgcccgagg tagcgcctgc
     4561 ggtcacggcg gaagggcacc gggagagtga gactcgttga ttggttctcg aactcagaca
     4621 tcagacagga atacatcaaa atggcacatc cgcgattcat gatctccaag agccctggtg
     4681 gccaattcac cttcaacctg accgctgcga atggcgcggt gatcctcgct agtgaacgct
     4741 acacgtcgca cagcgccgcg gttgcggggg caagatcggt tcaagcccac gcgggcgatg
     4801 acgggaacta cgaacatcgg acggcgagtg acgggcggca ctacttcgtc ctgatcgcag
     4861 ggaatcacga gatcattggg acgagtcaga t
//
LOCUS       DASTKU010000512        32833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_97994,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000512 DASTKU010000000
VERSION     DASTKU010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 32833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32833
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97994"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2357)
                     /locus_tag="VFQ61_17600"
     CDS             complement(<1..2357)
                     /locus_tag="VFQ61_17600"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9956330.1"
                     /translation="MTRTRVWLSAILAIVVVLSTLFTVWQGLEPPPAPTEIRAGSPLH
                     ERLSRRVLLVVVDGLRYDVATDPARMPLFSAAMRAHSSAEIWAGRVSMTSSAVLSYGT
                     GQHGRLEQIARNLHARAPEHNAWFANAHQAGLRLGAVGDTAWKQLYEAQLDWVRPDPD
                     GVAIDVDFNPKTFADAREIRAKGPDFLVAHFVTPDHQGHAYGILSERYTRHIRAFDAL
                     LAEFLSEFDASWTVFVTSDHGAADSGTHGTDVPVQRRSPIYAYGPGLQAGVHLPRLEQ
                     VDLSATLPVLLGVAPPAHGSGRLIGEWLALDEATRAELACRHAERVVRYASVVAKGEL
                     DASLVSACAHTRAPSSRAAAAERAVREADRAIGEVTGVASRGVMIWLWATAGCVLLFG
                     LALLPMSQLRAWPSVASMLLFGVLLVLFVERLPSVFPNVARVALFVVSNALLLAILLR
                     PKLSIRALSRATPHAAALIPGALVAAYPANARPESFVITALFGVLAIFRPSFFRGNGL
                     EPGVVAREPPFEWPRGVEVITCVVCLLLLLPVALKENGTYSVWFVAPQTRAALLTLLV
                     TGLLTLQVLVREHADALREQRTLRQRILFTSAACAALIPPLWLRGSVGPWLGRTAWLG
                     SAALLVWALQRGHKRTAMLLGLCSLCWVARDFEVFALLGALTLAWVIGTAWARAELSY
                     EKVLLACLLSVALCYALRIGLQDGLEFGGLDWGAGAFNDPSVPIWVVGGALVYKYVVA
                     CALVNLALFREFSPLLQLRLMSAILLCWVVRGLALAGMFVIAGNS"
     gene            complement(2388..3521)
                     /locus_tag="VFQ61_17605"
     CDS             complement(2388..3521)
                     /locus_tag="VFQ61_17605"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET9956331.1"
                     /translation="MRVVLDARYAGPRPSGIGNYVRPLGARLPKLLPHASFRYWIREG
                     TEPLSAASNVQHVPVNAVPAGLASLLWPARLDDFEEGDVFHAPANILGFGLPRASVVT
                     VHDVMWIEHLAWCQPRPYLRPISKAYYTTGIRRALRSAARILTVSKASADAIRRVEPS
                     AAARTVVTPNACEPHFKPAAEPERARELAARCIGSDEPYFLVVGQNQPSKGHAYAVAA
                     FAEAALESHRLVLVQRLESGRGLHALCERLGIAQRVTFAESLPFEALLALLQSATALV
                     QPSLAEGFGLPVLEAIAAGCPVLASDIPPFREILGQVGLFSSPTNVGELAQNMRTAAA
                     EPALLSELSARGLERAKSFDWDRTAQLTAEVYAEVARGNRARV"
     gene            complement(3606..4493)
                     /locus_tag="VFQ61_17610"
     CDS             complement(3606..4493)
                     /locus_tag="VFQ61_17610"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9956332.1"
                     /translation="MQRRDPRFGRGEHQISEEPSVNVDQVKLELLRLFRSPDYKPPVL
                     PAVAVELLALTQKRSVNVAEVSRLLSKDPMLAGQVLKIAQSAAYSRGEPVRSLDDAIV
                     RLGLSRISDLFLRAALEMKVFRAAGYDHEMEALRLHSAFTAEAVRIVCRHSIGLDDYA
                     FLCGLLHDVGIAACILALTELAKKNKTKLDFEHVWPGLREIHAACSEMLAKIWQLPAD
                     IALVLSLHHTMVFEGRVHPLAAAVAVADGLADEVGFGFHGEMTAVQTESAAQMLGLDA
                     GTLDRIRSATVDLAKSFKS"
     gene            4618..5736
                     /locus_tag="VFQ61_17615"
     CDS             4618..5736
                     /locus_tag="VFQ61_17615"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET9956333.1"
                     /translation="MRLSHAVCSLLPLLASVLATGCKKPEKQAAETQTVQSVPVPEAT
                     AAAVKGPSADELTLPTLPPVPIPADNPMTEAKVKLGHQLFFDKRLSVDGSRACYSCHL
                     NEDGTGGHDPTAIGPKNKALPRHSPVLWNVAYLPKLYWDGRSDSLEAQGTAAWAGGNM
                     GVGKENLEAKAKEIGKIAGYKKQFKEVFGSPDPTPANIVAALSAYERTIICDNTAYDK
                     YAKGDKSALNDSQKRGLELFMGKAACATCHTPPHFSIAALGKDGAFFNTGMGTQDKKE
                     EEIDKGRMAVTQKDSDFAAFKPPMLRNVTKTAPYFHNGSQPVLKEAVRFMASGGYKNK
                     NLTPLLADRKLSDAELDSIVSFLGALECPGKLEEPKLP"
     gene            complement(5755..7047)
                     /locus_tag="VFQ61_17620"
     CDS             complement(5755..7047)
                     /locus_tag="VFQ61_17620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9956334.1"
                     /translation="MPVSSAHFRVIVIGGGPSGATVASLLARDGHRVLLLEKEHFPRF
                     HIGESLLPCSTPLLEQLGVLEQMERVFLPKHGAEFVTADGSLERRYAFGDGSVPGPSQ
                     AFEVERSEFDKILLDNAARIGVDVRQGVQVTGVEFSDTEVRVEARSAEAGPLKFTAEF
                     LVDASGQSSFLAGRLKLRSMDSQLRNYSVFSHFVGAERHSGLREGDISIVLVPEGWWW
                     VIPLRNDRTSVGLVAPSASLKGQKPDTRFLESKIQSTPYLARRLARAERVADVRALSD
                     WSYTSTRFVGQRFALVGDAAAFIDPVFSTGVYLGMSGAFRAARAIDQVLRRDRTAPRA
                     LADYERFVRRNVQIYRKFVHGFYDPAFVEVLLQPSDWMGLRAAITSLLAGFGVESADV
                     RWRASLFRMVARANRHLRLVPRLPERRSARPGVLSPFR"
     gene            complement(7082..8143)
                     /locus_tag="VFQ61_17625"
     CDS             complement(7082..8143)
                     /locus_tag="VFQ61_17625"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HET9956335.1"
                     /translation="MAQIHDPLFALPDHSGIGGQIGPEPEDFYVEEIPSYLPSGEGEH
                     LYVLVEKRGISTPELVRRLARAANVDSRDIGYAGLKDKHAITRQWLSLPKSALDPAQW
                     GTLENVEVLSQSRHANKLRTGHLSGNRFRIRLHGVADGALPTAQALAEALRQSGVPNY
                     FGGQRFGRGGENLSRALDWLRGGARARLSSFLVKLYPSVVQSEIFNRYLSLRRSEGLD
                     RLLPGEVVRLNGSHAQFVVEEPEREQPRLAARDIHPTGPIPGPKMRAAQGRALELEAL
                     AMQEADVSDAVMQVLGRFAPGTRRDLLVYPEELCVSESKPGQLELSFALPAGSYATVL
                     VREFTRRPLIASDLDERES"
     gene            8316..9563
                     /locus_tag="VFQ61_17630"
     CDS             8316..9563
                     /locus_tag="VFQ61_17630"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET9956336.1"
                     /translation="MRSEVPAIQKTGLVLSGGGTRGAYEVGVVSGIMQVLKPKLGDPP
                     VFRIFSGASVGAINTTFLAANAELPDHGVSKLSQLWKSLDLSEHARIRPLGLARLPGS
                     LRSRLKSLAGEKLGTSLLDTRAIDLLVRRSIDWDKLHRVIDSGRVLSLLVAALHVASG
                     RTIMFSEHAPGTTVIPTRDERRATVFERITPSHVLASAAIPLLFPSQRIGKHYYCDGG
                     LRFNTPIAPAIRAGSDRLIIVSVRHSRTARDMAAVEEADTGEGRDLGPAFLIGKLLNA
                     LLLDPVAYDLQVLERLNQVMDVLEQSLSQAELDRVQRVWIEHRGLPYRKLKTLVFTPS
                     RDLGEIAGDYLRTRMRTDHLRPLARYLMDRAVQASPHGEADWASYILFDGSFATELIA
                     IGERDALERRDEILEFFGRTGQP"
     gene            9727..10539
                     /locus_tag="VFQ61_17635"
     CDS             9727..10539
                     /locus_tag="VFQ61_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956337.1"
                     /translation="MNKARLHPGFLLGFVLGTLSGAAACGSSSGGELFSAGNNPAGGT
                     RSVGTPSGPQTSGVFGAGGTANASGGSASGSSSVNASSAGGANSSGGSNFNASGSGGA
                     TDAAGNAGTSSSGGSSGGATAQAGTGGAPAGGSAALGGASSAGAAVGGTSTGGGAAGA
                     TMTNAGSSAVGVCATFSSDIATLTPTLQAAQTCDPKVMNACAVLEQGDCGCPIQAQAN
                     RQLLEPLRVRLQDRPVGCRCPEICPSSQGSGSRGTLSCMPAPSGLGSCVFLK"
     gene            complement(10554..12257)
                     /locus_tag="VFQ61_17640"
     CDS             complement(10554..12257)
                     /locus_tag="VFQ61_17640"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9956338.1"
                     /translation="MSIEVNFSPNSCDFGAVPARRKKYVRVAVTVGDPFSAGRNVRVK
                     VSRPVTTPDQFVPLAALGWEAVGTNAVQSPLVRIGGALLPTVYLLVSLSESLVLGPLA
                     GSLQLSVVDEAGNPLPDSERVLNVSGSISAAQTVTLALVIDRSTSMGELLAGESRVRR
                     ALSAATALLALMEDTDRLGIVSFAAEATELLSIGTLNQMIGTSTRRQVAQTLLDPVNL
                     NPAFLTPPGQPTCIYAGVQKGRIMLYLSPGADKNLIVLTDGLEDYGNSVSKLPQLPDT
                     YAIGVPSLNTTSLSWLTSANGYLSVDGAVTGDVLFKVQKYAAQIFANVSNTTIVVDPD
                     QALPASPQAFFDLEFSTTELDEEFKVVVFAKEPERLTLEPITAPSPHSKAETATAADL
                     STGPARSYESPRSLKVQSVERSDGVMLTRLSNDPDPAGLRPASRSVRLQRIGKDEKPS
                     TTPFSYLVIAKSDLVLDAQVHASSPRVGGELLLTAVLFEAGLPITERARVQAEVRYPE
                     GVCSELKLSEDIEGRFTARLETLRPGLYEVRFKAQGKTVFGTAFHREQLRTVAIQPAR
                     C"
     gene            complement(12927..13217)
                     /locus_tag="VFQ61_17645"
     CDS             complement(12927..13217)
                     /locus_tag="VFQ61_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956339.1"
                     /translation="MQAEPSLFAGPVHVRPGESRALNTKSYELGRYMQQRESHATVHD
                     HWPARRPELYSPECAPDQHGSVHSSLGFEHLELDILRAELIEARLPARDQCV"
     gene            complement(13542..14216)
                     /gene="mutH"
                     /locus_tag="VFQ61_17650"
     CDS             complement(13542..14216)
                     /gene="mutH"
                     /locus_tag="VFQ61_17650"
                     /inference="COORDINATES: protein motif:HMM:NF003458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HET9956340.1"
                     /translation="MSRIPTPQTEAELLERAERLAGDSLSGVAERLGHAVPANLNHHK
                     GWVGHLIERALGASAGSRDAPDFEALGVELKTLPVDAAGRPFESTFVATILLSELTEL
                     EWEQSRPCRKLTRVLWVPVQGDRRIPLNQRRIGTPFIWSPDADEGSALRFDWDELAGL
                     VARRGVEAVTGHVGQFLQIRPKARDASVRFLGSDNDGAPLRTLPRGFYLRPVFTHRIV
                     RHRFGL"
     gene            14301..15947
                     /locus_tag="VFQ61_17655"
     CDS             14301..15947
                     /locus_tag="VFQ61_17655"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9956341.1"
                     /translation="MRKTVALVLCSLFLACQATRDLGLATEARSELRSQTELRSQAEL
                     RSQTELRSQTERSAPAHVPVSLPAPSGAVALPASALPGTAPHSPPASEPPRLYAKTRF
                     VWVYPRPDAQAQWLGFLRSGSSVELRGTKPQAGPGCAAFYPIAPEGFVCGEGNRATLD
                     HDDPVYRALEPYALRSDKAYLHQYGESLGLPRYHSLPDERAQQERETDLRSHLKNVER
                     ARQGSVPTVLTGVDVSLPASTLPGFPTLPWPVFEDRKDLKPRSTVAFHTETRVGDRGF
                     LLTGDFAWVPKDRVRPYPKIEFHGVELAELRLPLAVFRKEGTLKYARDAGGSFHALGD
                     TPENHLARLSFVPLTGKLESQADTQYLEVVGGEYWIRQSDAVVPTPRETTPWGTRLAD
                     PNPPAPPKGGRGTWVEVSIDQGWLIAFENTRPVFVTLISPGRGGAARPGEDPLERSAT
                     PTGTYPITGKFLTSTMEAPGEFVHSDVPFAQNLTGPYALHAAYWHDNWGNPQSGGCVN
                     LSPLDAKWLFDFTEPSLPKGWHGVRWIPQNGPATIVILHR"
     gene            complement(15982..17082)
                     /locus_tag="VFQ61_17660"
     CDS             complement(15982..17082)
                     /locus_tag="VFQ61_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956342.1"
                     /translation="MTRARAKQKPEATALGQGNDLLREAELIRARDDLLKKIGRKRRE
                     AEKAEQAVAASAELVRSRFPALMTQTVRLSQEIHALFSALLRRDDLPRAAQRAVRQVY
                     HSLRVSGRLAPEEGSDEDESDDESFHEPSDDFDAEFDAEQAPPRTGRRGGAAPGPEQR
                     VGSASPAGARPGNSGLKDLFRKLAVALHPDRTRDEREREERTRIMKEVTRAYEEGDLA
                     RLMQIEASLVNGGPDVTAGPASEVRAQRLNKLIEELKTQLRELSKQVRELRRASREFV
                     FAGGRKGISAAEALELAERELGLLREIHEYVLAFSERRMSLHEFMAGPPALREEEQAD
                     EIPEELAELIFEMFSDAPAPSRRSGGKKRRRR"
     gene            complement(17147..19144)
                     /gene="nadE"
                     /locus_tag="VFQ61_17665"
     CDS             complement(17147..19144)
                     /gene="nadE"
                     /locus_tag="VFQ61_17665"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HET9956343.1"
                     /translation="MKLIKVAAAALNQTPLDWAGNCANILAVLAEARKQGVSVLCLPE
                     LCITGYGCEDAFLAPGTQRTALEVLAEVQRESRGIVVCVGLPLRLDKAVFNACALLAD
                     GKLCGFVAKRALAGDGLHYEPRWFKAWPRGKVATCQVLDTEARVGDVLFDVGGVRLGF
                     EICEDAWIAERPGGRLARHGVDLILNPSASHFAFGKHEVRRRLVLEGSRAFGVSYLYA
                     NLLGNEAGRAIYDGDALIATAGAICAESRRFSYRPYELVSAVIDVELTRTHHTRLTSF
                     RPALSKNISRVAVPFDYPACRLERNEIEGEAWEGSPRIREEEFGRAVSLALFDYLRKS
                     RSRGFVVSLSGGADSSAITCLVASMVELACKDLGVAGVQARLGYLRGLEACRSTREIV
                     GRLLTTLYQSTRNSGPVTRAAASGLAEAVGARHFELEVDSLVQGYVALGEKVFGRSLA
                     WETDDIALQNIQARVRAPSAWLVANVQGALLLSTSNRSEAAVGYATMDGDTAGSISPI
                     AGIDKAFLRRWLVWLESEGLSGLAPIPALQAVNQQSPTAELRPPGEGQTDEGDLMPYD
                     VLDAIERAAIRDKRSPSEILEALRLDFPERADTLLVAWITRFFRLFCRNQWKRERYAP
                     SFHLDDESLDPKTWYRFPILSGGFERELGELSAALEATSRP"
     gene            complement(19334..20305)
                     /locus_tag="VFQ61_17670"
     CDS             complement(19334..20305)
                     /locus_tag="VFQ61_17670"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9956344.1"
                     /translation="MKAKLLLIDDSEAQGNHIRSSLEHLGYSVEWARSGVEGLRLART
                     EKPDLVLLDVVMADIDGFAVCRWLKMHADTRDIPIIMLTVRTALADRVEGLNVGADDY
                     LAKPFETEELEARIFASLRAKAANLELRERNQELEAMLHHVEALAITDPLTGLFNRRR
                     FADVLKREYAVTRRYQNTLSCLLLDLDHFKRINDHYGHDAGDRVLKELAQKVAENLRE
                     VDVPARYGGEEFAVLLPHTRKEDARIVAERLRRAIESIQLTFDGKPERVTVSIGVAGN
                     TDVASGKAEDLVKAADVALYEAKRLGRNRVVVYDAGSAEPPTSDRSA"
     gene            complement(20585..22543)
                     /locus_tag="VFQ61_17675"
     CDS             complement(20585..22543)
                     /locus_tag="VFQ61_17675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9956345.1"
                     /translation="MSTPPDSEVASESLIGRVLVDRYRVEALLGEGGMGTVYRVEHVH
                     MRKAFAIKVLHRSMTQMPEIVERFEREAVAAGRIQHQHVASASDFGRLSDGSFYLVLE
                     LIEGQSLASLLKDQGTLPRTRVLTIARQIADALSAAHAAGIVHRDLKPENIMLTNRDG
                     VPDFVKVLDFGIAKVPVESRGGDRQLTQLGMVFGTPEYMSPEQARGAEVDGRADLYTL
                     GVIMYEMLSGKSPFRHEDLVAVLMRQITAEPDPLPETVPAPVARFVFELLRKNPDERP
                     QTAVVVRERLDELLALGRAEFPSSSGAVTAITAPALATAAPGNAAAGRAFGLALADVR
                     DVVSRAQLQSALRASLAWGQHKLAALRLERRLSIGRYTLPLSLLVLISGLVLVVAGLA
                     TTLLTMGPSAAPSAHAEAPKAEAAAAPPVNVLALVTRAETGDAKAIAELETANSAEHR
                     SLVEWRALGRGYCRLGDAKNCLRSYSAALSQRPELAKEPLLLADLRALADRPDVGGQV
                     LQLAARSLGPAGADLLYDVWERGKASNTGQSQQAKALLEVEPARSQASPNLKAFWALQ
                     LALKKPRCGDLKRLLAEREQDFDERSVPLLNRLTERQGCGILGLGDCYSCLRGGELSR
                     ALAQAKSHARPSFGQTSSIPTGSAAGAR"
     gene            complement(22633..23745)
                     /locus_tag="VFQ61_17680"
     CDS             complement(22633..23745)
                     /locus_tag="VFQ61_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HET9956346.1"
                     /translation="MDNATRLTLELERAALHAIRRTFDDLNGCFFRWELRAPAFELSD
                     TRAHLGRWVSSTRVIELSRALLLEHGWGVLTEVLKHEMAHQYVDEVLGVRDEPSHGPN
                     FQEVCRARGFDAKAHGLPDAEPTSDARARVLDRIAKLLMLAQSQNEHEAQAAMSAAQR
                     LMLKYNIDSVSEPGERQYTYRHLGVPTGRVSEAERILAALLGDFFFVEVIWVPVYRPL
                     EGRRGSVLEVCGSLENVELAAYVHAFLMHTSEHLWQTHRRSRGLQGNGGRRSFVAGVM
                     SGFRDKLEKERHTQKQQGLVYAGDPKLERYFRSRYPRIRHTHYRSQASHAAYGEGRSA
                     GQKIVLHRGLRSENATSGGTNGSASARPVGLLNGRS"
     gene            23794..26058
                     /locus_tag="VFQ61_17685"
     CDS             23794..26058
                     /locus_tag="VFQ61_17685"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HET9956347.1"
                     /translation="MSKDEEALLYHSEGRPGKIEVVWTKPVASQLDLSKAYSPGVAAP
                     CRAIAADPDKVDLYTARRNLVAVVTNGTAVLGLGNIGPRAAKPVMEGKGVLFKRFADI
                     DVFDIELDAQDPEEVVRIVRALEPTFGGINLEDIRAPDCFVIERALREQMSIPVFHDD
                     QHGTAIISAAAVRNAAYLQEKNLADLKVTCVGAGAAATSCMRMWVRAGVRRENITMLD
                     VGGVLHLGRPDLDQFRRDFARPLSDPRRTLQEAIVDADVLIGLSVGGLVTPAMLKTMA
                     KRPIVFALANPDPEIGYREAIAARPDAIVATGRSDFPNQVNNVLGFPYIFRGALDVGA
                     RAITEEMKVAAAEALAELAREEVSDEVLRAYGRSRMRFGRDYIIPKPFDTRVLYWVAP
                     AVARAAQESGVARFSIDVDDYRRRLFQRISPTRRVLWNILESARSSPRRIVYPESEHD
                     GILRAAYRVMEEGIAHPILIGARDRILEQASHLGVDLSRATLVDPRESDKLESYADAF
                     WAKRQRKGLTRHGALKELRRSRTLFGMVMVDQGDADGIVAGARQDYPATIRPALQVIG
                     VRDGVTHAAGIYMVVSKSGVRFMADATINIDPDADTLAEIAILAAETVADLGLEPRVA
                     LISFSNFGDAPHPSSKKVAEAVAIVKRRRPDLMVDGEMQVNVALDEEARAPYPFSKLK
                     GSANVLIFPNLDAGNAAYKIVAASGEADVVGPIVMGMRRPVNVLQQGASVDAIVHMTA
                     ITVARANRLDKDSR"
     gene            26062..26508
                     /locus_tag="VFQ61_17690"
     CDS             26062..26508
                     /locus_tag="VFQ61_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956348.1"
                     /translation="MQLSRLVPALLVLASGTGCGKFKRSKECGAVAGAVSTFVSASAP
                     TPIGKGQPASITPTDLARDARATARRYNELDQRLESLHVQSADLAPRVTHYRELARQA
                     AIALEDAATALEHSDAETARRKRVEFDAVARSEPALVAEINNICRR"
     gene            complement(26675..28789)
                     /gene="uvrB"
                     /locus_tag="VFQ61_17695"
     CDS             complement(26675..28789)
                     /gene="uvrB"
                     /locus_tag="VFQ61_17695"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET9956349.1"
                     /translation="MPFALRSDFSPKGDQPAAIEALVRGFKEGATRQTLLGITGSGKT
                     FTIANVIQAMQRPTLIIAPNKTLSAQLYSEMRDLFPDNAVSFFVSYYDYYQPEAYIPT
                     TDTFIEKDAIINDQIDRMRHHATQSLLSRSDVIIVASVSCIYGIGSAETYSGLLIEIV
                     KGEELRRDELLRRLVDIQYERNDTDFHRGTFRVRGDVVEIFPVYEEALAVRVEFFGDE
                     VESISEVDPLRGRILRSLDKYAVFPGSHYVTPRQQLQRSIQGIREELKERLEFFDREG
                     RFLEKQRLGQRTLYDLEMLEQMGFVQGIENYSRHLSGRAPGDPPPTLVDYFPEDFLLV
                     IDESHQTVPQIGAMYRGDRSRKETLVEYGFRLPSALDNRPLRFEEFEKHMRNVVFVSA
                     TPGDYELKESDGRIIEQVIRPTGLLDPEIQVRPVAGQVDDLFGEIKTRIAAGDRVLVT
                     TLTKRMAEELTEYYSELGIKVRYLHSDIDTLERVEILRDLRAGEFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSPRSLIQTIGRAARNSRGQVFMYADRITDAMKIAISET
                     ERRRELQQSYNREHGITPETVRRAILEMTPSSGESDYYAVPRGSSKKANAPAESGASK
                     SATNDENLAERILELRQKMFAAAENLEFETAARLRDELRRLSAGNPDVVPPAAAPMAP
                     ARAKAAAKGGARKGAAAGRRRR"
     gene            complement(29037..29432)
                     /gene="rpsI"
                     /locus_tag="VFQ61_17700"
     CDS             complement(29037..29432)
                     /gene="rpsI"
                     /locus_tag="VFQ61_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET9956350.1"
                     /translation="MPSAQSTYATGKRKTAVARVWIQPGSGAITVNGKNVDEYFERET
                     SRMVVRQGLELLELQDKYDVVASVAGGGHSAQAEAMRHGISRALTSEDLERRRTLKRA
                     GYLTRDDRKKERKKYGQAGARKRFQYSKR"
     gene            complement(29470..29904)
                     /gene="rplM"
                     /locus_tag="VFQ61_17705"
     CDS             complement(29470..29904)
                     /gene="rplM"
                     /locus_tag="VFQ61_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET9956351.1"
                     /translation="MSTYVAKPAEANLTRKWFVVDATNQPLGRLASKIASVLRGKHKP
                     VYTPHVDTGDFVIVVNASKVKLTGSKVTDKMYYRHSGYPGSIRGESYRDVVVRHPDAP
                     ITLAVKGMLPKNVLGRQMLEKLKVYGEAEHPHQAQKPEALNV"
     gene            30407..32353
                     /locus_tag="VFQ61_17710"
     CDS             30407..32353
                     /locus_tag="VFQ61_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET9956352.1"
                     /translation="MPPTGNNRLLERLASDRVITPEQLEAAANWMARNGDRAEEALLE
                     LGVLEEAALLKYLAALHRTRFVSTEKLAKAEIDRLTLDKVPKKLAERDCVFPVLYDPQ
                     AASLSVVTPDPDNAIALQDLQLASGVKDVRAFVGRPRAVKAAINKAYNGDIHAFANLD
                     REAHAQFTTMLNVYERNLVSEESLAVAVAREAVGERVLTGADLEQGRRAASGGGRGIT
                     DDSYLETLNVLVNLLENSRADLRGHSAQVARLMKKVGERIGVSEISRAALQAAGYLHD
                     IGKMGTYHLTALNVAEYEGHRTSAQKQYRAPLRLLEAVHLPAEVTQAIHHMYERYDGQ
                     GLPDGISGKEIPLGARLLAIADTYADLTQNPRNPFRKTLRPVQACEVLERYKGGIFDP
                     NLVDLFKHTVTGEDLKARLLANRHRALLIDPDPEETTVLELRMIEQGFEVFQARNTEA
                     ALKQLEKGEIEIVISEVDLAPGDGFALLSQVRGQGYGQGLPWVFLTSRAGRAEAQRAF
                     ELGAADFMAKPVATDLLVAKLKQILERGAHGTRTAARGVSGSLSEMSLPDMVQVLWHG
                     RKTGSLKIRSGTNTGEIHFVEGNIYNALYAALRGEEAFYAMLALTQGEFVLDPNFRAP
                     QQVVQATPEALLLEGMRRLDENER"
     gene            32543..>32833
                     /locus_tag="VFQ61_17715"
     CDS             32543..>32833
                     /locus_tag="VFQ61_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956353.1"
                     /translation="MPPPVLGFNTNIRYRGLTFHVQSEDSGVRTPRIMTHVFADGGRI
                     VKSARTDYSAFLGREDLREVVRKMMKEQHKAMLIALRSGEFDAQLEKACGTLP"
BASE COUNT         6254 a        10967 c        10017 g         5595 t
ORIGIN      
        1 tagctattgc ccgcgatcac gaacatgccg gccagtgcca agccacgcac cacccagcac
       61 agcaaaattg cgctcatcag ccgcagctgg agcaagggag agaactcgcg gaacagcgcc
      121 aagttcacca gcgcacacgc caccacgtac ttatagacca gcgcgccccc gacgacccaa
      181 atcggcacgc tcggatcgtt gaaagcacct gcaccccagt ccaagccgcc aaactccaag
      241 ccgtcctgaa gaccaatccg cagcgcgtaa cacagagcca cgctgagcaa gcaggccaag
      301 agcaccttct catagctcaa ctccgcgcgt gcccaggccg ttccaatcac ccacgccaag
      361 gtaagtgcgc cgagcagcgc gaacacttcg aagtcacgag ccacccagca caggctgcag
      421 agacccagaa gcatcgccgt gcgtttatgc cctcgctgca gggcccaaac cagcaacgct
      481 gcgctaccga gccacgcggt tcgtccgagc cagggtccca ccgagccgcg caaccacagc
      541 ggcgggatca gcgccgcgca ggcggcgctg gtgaagagaa tcctttgcct aagcgtcctc
      601 tgctcgcgca acgcatccgc gtgctcgcga acgagcacct gaagcgtcaa cagacccgta
      661 accagcaacg tcagaagtgc cgcgcgggtc tgaggcgcga cgaaccaaac cgagtaagtt
      721 ccgttctcct tcagtgcgac cggcaaaagc aacagcaggc atacgacgca ggtgatgacc
      781 tcgacgccgc gaggccactc gaagggcggt tcgcgcgcga ccacacccgg ctcgagcccg
      841 ttgccgcgaa agaagctcgg acgaaagatt gccaaaacgc cgaataacgc cgtgatcacg
      901 aacgactcgg gccgcgcgtt ggccgggtag gccgcaacga gcgcgcccgg aatcaaagcg
      961 gcggcgtgtg gggtggcgcg cgacagcgcg cgaatgctca gcttgggcct cagtagaatg
     1021 gcgagcagca gcgcgttcga aactacgaat agcgccaccc gcgccacgtt cggaaacacc
     1081 gagggaagcc gttcgacgaa cagcaccagc aacacgccga acagcagcat cgaggcgacc
     1141 gagggccaag ctcggagctg gctcatgggc agcagcgcga ggccgaacag caacacacaa
     1201 cccgcggtgg cccacagcca gatcatcacc ccgcgagacg cgacacccgt aacctccccg
     1261 atcgcgcggt ctgcctcgcg cacggctcgt tctgcggcgg ccgcccgcga gctcggcgct
     1321 cgagtgtgcg cgcaagcgct caccaggctc gcgtcgagct cgcctttggc caccacggac
     1381 gcgtacctga cgacgcgctc tgcgtgacga caggcgagct ccgcgcgcgt cgcttcgtcg
     1441 agcgccaacc actctccaat caagcgcccc gaaccgtgcg cgggcggagc caccccgagc
     1501 agcaccggca gcgtcgcgga caaatcgacc tgctcgagtc gggggagatg cacgcccgcc
     1561 tgcaggcctg ggccgtaagc atatatggga gagcgacgtt gcaccggcac gtcggtgccg
     1621 tgggtacccg agtctgcggc gccgtgatcg ctcgtcacga acacggtcca gctcgcgtcg
     1681 aactcgctca agaactcggc gagcagcgca tcgaacgcac gaatgtgacg cgtgtagcgc
     1741 tcggacaaga tgccgtaagc gtgcccctga tggtccggcg ttacgaagtg cgccaccaag
     1801 aaatcggggc ccttcgcgcg gatctcgcgt gcgtccgcga aagtcttggg attgaaatcg
     1861 acgtcgatcg caacaccgtc cggatccggt cgcacccagt cgagctgagc ctcgtaaagc
     1921 tgcttccagg ccgtgtcccc aaccgcaccc aaacgcaggc ccgcctggtg ggcattcgca
     1981 aaccaggcgt tgtgctcggg cgcccgtgca tggaggttac gagcaatctg ctcgagccgc
     2041 ccgtgctgac cggtgccgta gctcagaacc gcgctgctcg tcatcgagac gcgaccggcc
     2101 cagatttctg cgctggagtg tgcgcgcatc gccgccgaaa acagcggcat tctcgccgga
     2161 tcggtcgcga cgtcgtagcg cagcccatcc accacgacca gcagcacgcg acgagagagg
     2221 cgctcgtgca gcggcgaacc cgcacgaatt tccgtcggcg ctggcggcgg ttccaggcct
     2281 tgccaaaccg tgaagagcgt cgagagcacg accacgatcg ctaaaatcgc actcagccac
     2341 acgcgagttc gcgtcatgcg gagtgcgccc tcgcgccgta gcgagcttca gacccgcgca
     2401 cggtttccac gcgccacctc cgcataaact tccgccgtca gttgcgcagt ccggtcccaa
     2461 tcgaacgact tcgcgcgctc gagcccacgc gcgctgagtt cggacaacaa ggccggctct
     2521 gcagcggcgg ttcgcatgtt ttgcgccagc tccccaacat tcgtgggact cgaaaacagg
     2581 ccgacctgtc cgaggatctc tcgaaacggc ggtatgtcgc tggccagcac gggacaaccc
     2641 gcggcgatgg cttcgagcac gggcaaaccg aagccctcgg ccaacgaggg ctgaaccaac
     2701 gcggtggccg actgcagcag cgcgagcagg gcctcgaaag gtaagctctc ggcgaaggtt
     2761 acgcgctgcg cgatgccgag ccgctcgcac agcgcgtgca gcccgcgccc gctctcgagc
     2821 cgttgtacga gcaccagacg atggctttcg agtgcagctt cggcgaatgc ggcgacggcg
     2881 taagcatgcc ccttcgaagg ctggttttgc ccgaccacca ggaagtacgg ttcgtcacta
     2941 ccgatgcagc gtgcggcgag ctcacgcgct cgctcgggtt cggccgcggg cttgaagtgc
     3001 ggctcacagg cgttgggcgt cacgaccgtg cgagccgcag cgctaggctc gactcgacgg
     3061 attgcgtccg cggacgcctt ggagacagtc agaatgcgcg ccgccgagcg caaagcgcgg
     3121 cgaatcccgg tcgtgtagta ggccttgcta atcggcctca agtacgggcg cggctggcac
     3181 caagcgaggt gctcgatcca catgacgtcg tgaaccgtca cgacgctcgc gcggggcaat
     3241 ccgaaaccga gaatgttcgc gggcgcgtga aagacgtcgc cttcctcgaa atcatccaac
     3301 cgcgcgggcc acaacaagct cgcaagcccc gccggaacgg cattgacggg cacgtgctgc
     3361 acgttactgg ccgcggagag cggctccgtg ccctcgcgaa tccaatagcg aaagctcgcg
     3421 tgcggcagca acttcggcag ccgcgcgccg agcggacgca cgtagttccc gataccgctc
     3481 ggtcgcggcc cggcataacg ggcatccaag accacacgca tgccgcgccc tcctagcacg
     3541 aattgctgag tccgggagct atcgggagcc atcggacgca gcacggagcg cgcccgacgg
     3601 cgcgctcacg acttgaagga tttggccaaa tctacggtcg ccgagcgaat tcgatccagt
     3661 gtacccgcgt ccaggccgag catctgggct gcggactcgg tctggacggc cgtcatttcg
     3721 ccgtggaacc caaagcccac ttcgtccgct agtccgtccg ccaccgctac cgcagctgcc
     3781 agcgggtgca cgcgcccctc gaacaccatg gtgtgatgca ggctcagaac cagcgcgatg
     3841 tctgcgggga gctgccaaat cttggccagc atctccgaac atgccgcgtg aatctcgcgt
     3901 agccccggcc acacgtgctc gaagtcgagc ttcgtcttgt tcttcttggc cagctccgtc
     3961 aacgccaaga tgcacgccgc gatccccacg tcgtggagca gaccgcagag aaacgcatag
     4021 tcgtccagac cgatgctgtg tcggcacacg atgcgcaccg cctcggccgt gaacgcggag
     4081 tgcaggcgca acgcttccat ctcatggtcg tagcccgccg cgcggaacac cttcatctcg
     4141 agagcggcgc gcaagaacag gtctgaaatg cgcgacagac cgagacgtac gatggcatcg
     4201 tccagagagc gcaccggctc tccacgcgag taggctgcgc tctgagcgat cttgagtacc
     4261 tgcccggcta gcatcggatc tttgctcaac agccgcgaga cctctgccac gttcacggat
     4321 cgcttctgcg tgagggcaag tagttccact gccaccgcgg gcagaacggg aggcttgtag
     4381 tccggagagc ggaacaggcg cagcagctcg agtttcacct gatccacatt cacggacggc
     4441 tcttctgaga tctggtgctc cccgcgtccg aaacgcggat ctcgacgctg catccactca
     4501 agaacgcaac tttcttacac gacataagga gattcggggc gaaacacctc atgcatcatt
     4561 tgcgcggtcg cgcccggttt attgccgcgc gaactcaact cgggtatcgc gggtcgcatg
     4621 agattgtcgc acgctgtatg cagcttgctg cctttgctcg cctcggtgtt ggcgacgggc
     4681 tgcaagaagc ccgagaaaca agccgcggaa actcaaaccg tacaatccgt gccggtaccg
     4741 gaggcaaccg ccgcggcggt gaaggggcct tccgcggacg agctcacgct gccaacgctc
     4801 ccgccggtgc cgattccggc ggataatccc atgaccgaag ccaaggtgaa gctcggtcat
     4861 cagctgttct tcgacaagcg tttgagcgta gacggctcgc gcgcgtgcta ctcgtgccac
     4921 ttgaacgagg atggcaccgg cgggcacgac cccaccgcga tcgggccgaa aaacaaggct
     4981 cttccgcgcc atagcccggt gctctggaac gtggcctacc tgcccaagct gtactgggac
     5041 gggcgctcgg actcgctcga ggcgcaagga accgcggcct gggcgggcgg caacatgggt
     5101 gtcggcaaag agaacctcga ggccaaggcc aaggagatcg gcaagattgc cggttacaag
     5161 aagcagttca aagaggtgtt cggcagcccg gacccgaccc cggccaacat cgtcgcggcg
     5221 ttgtctgcct atgagcgcac gatcatctgc gacaacaccg cgtatgacaa gtacgccaaa
     5281 ggcgacaagt cagccttgaa cgacagccaa aagcgcgggc tcgagttgtt catgggcaag
     5341 gccgcgtgtg ccacgtgtca cacgcctccc cacttctcga tcgcagcgct cggcaaagac
     5401 ggcgccttct tcaacacggg catgggcact caagacaaga aggaggagga gatcgacaag
     5461 gggcgcatgg ccgtcaccca aaaggactcg gactttgccg ccttcaagcc gcccatgctg
     5521 cgcaacgtga ccaagactgc gccctacttc cacaacggtt cgcagcccgt gctcaaggag
     5581 gcggtccgct tcatggcctc cggcggctac aagaacaaga acctcacgcc gctgctcgcc
     5641 gaccgtaagc tcagcgacgc ggagctcgac tcgatcgtca gcttcctcgg cgcactcgag
     5701 tgcccgggca agctcgaaga gccgaagctg ccctgatatt tccgtagaaa cctatcatcg
     5761 gaacgggctc agcactccag gtcgggctga gcgccgttcc ggcaggcgcg ggaccaagcg
     5821 caggtggcgg ttggcgcgcg ccaccattcg aaacagcgag gcgcgccaac gcacatccgc
     5881 gctctccacg ccgaagccgg ccagcaacga agtaatcgcc gcccgcaaac ccatccaatc
     5941 gctcggctgc agcagaacct cgacaaatgc gggatcgtag aagccgtgca cgaacttacg
     6001 gtagatttgc acgttgcggc gcacgaagcg ttcgtagtca gcgagggctc gcggcgccgt
     6061 tcgatcgcgg cgcaacacct gatcgatcgc gcgggcggcc cggaaggcgc ccgacatgcc
     6121 caggtacacg cccgtggaaa acacgggatc gatgaaggcg gccgcgtcac cgaccagcgc
     6181 aaagcgctgc cctacgaagc gtgtgctggt atacgaccag tccgagaggg cacgcacgtc
     6241 cgcgacccgc tcggcgcgag ccaagcgccg agccaagtac ggtgtgctct gaatcttgct
     6301 ttcgagaaat cgagtgtctg gcttctggcc cttcaacgac gccgagggcg cgaccaatcc
     6361 aacactcgta cgatcgttgc gcaggggaat gacccaccac cagccctcgg gcacgagaac
     6421 gatgctgatg tcgccttcgc gcaacccgct gtgccgctcg gcgcccacga agtggctgaa
     6481 caccgagtag tttctgagct gcgagtccat gctccgcaat ttgaggcgcc ccgccaagaa
     6541 ggagctttgc ccgctcgcgt ccaccaggaa ctcagccgtg aacttgagag gcccggcttc
     6601 ggcgctgcgc gcctcgaccc gcacttcggt gtcggaaaac tccacgcccg taacttgaac
     6661 gccttgccgg acgtcaacgc cgatccgagc ggcattatct agtaagatct tgtcgaactc
     6721 cgaccgctcc acctcgaacg cctggctcgg tcccggcacg ctgccatcgc cgaaggcata
     6781 gcgccgttcc aagctcccat ccgcggttac gaactccgcc ccatgcttcg gtaggaatac
     6841 tcgctccatc tgctcgagca ccccgagctg ttcgagcaac ggagtgctgc acggcaggag
     6901 tgactcaccg atatgaaagc gggggaaatg ctccttctcg aggagcagca cgcggtgccc
     6961 atcacgggcg agcagcgacg caacggtcgc gcccgacggc ccgccaccaa tcacgattac
     7021 gcggaagtgc gcggaactca ctggcatcgc ccgctaccct acttgagaac gcgggcgcat
     7081 ctcaactctc gcgttcgtcg agatcgctcg cgatcaaagg gcggcgggtg aactcacgca
     7141 cgagcacggt cgcgtacgat cccgcgggca gcgcaaacga gagctccagc tgaccgggtt
     7201 tcgactccga aacgcagagc tcctcggggt acacgagcag atcccgccga gttcccggtg
     7261 caaaacggcc caacacctgc atgacggcat ccgacacgtc cgcttcttgc atggcgagcg
     7321 cctcgagctc gagtgcgcgt ccttgtgccg ctcgcatctt cgggcctggg attggccccg
     7381 tggggtggat atcacgagcc gcgagccggg gctgctcacg ctccggttct tcgacgacga
     7441 actgcgcgtg cgatccattt aggcgcacca cttcgccggg cagaagtcga tccaacccct
     7501 ccgacctgcg cagtgagaga tagcgattga agatctcgga ttgcacgacg ctcggataca
     7561 gcttgacgag gaacgaagag agcctcgcgc gcgcaccgcc gcgcagccaa tccaaagccc
     7621 gactcaggtt ctcgccgccg cgaccaaagc gctgaccgcc aaagtagttg gggacgcccg
     7681 actgcctcag cgcctcggcc agggcttgcg ccgtcggtag cgcgccgtcc gccacgccgt
     7741 ggagccgaat gcggaagcga ttgcccgaca agtggccggt gcgcagcttg ttcgcgtgcc
     7801 gcgactggct gagcacctca acgttctcga gcgtgcccca ctgcgccgga tcgagcgcgc
     7861 tctttggtaa ggacaaccac tgccgcgtga tggcgtgctt atccttcaag cccgcatatc
     7921 caatatcgcg cgagtccacg ttcgccgcgc gagccaagcg ccgcacgagc tcgggcgtgg
     7981 agatgccgcg cttctcgacc agaacgtaga gatgctcccc ttccccgctg ggtaggtaac
     8041 tagggatctc ctcgacgtag aaatcctcag gctctgggcc aatctggccg ccgatgccgg
     8101 aatgatccgg caatgcgaag agagggtcgt gaatttgagc catggtgtgc atccgacgag
     8161 cctcagagcg gcgccggtag gcgcagcgcc cagtgaggcg gcagcgctat gtaaggggct
     8221 cgcccagaag atgcaaacca ggagtatgat cgtcgcaaaa cgggggatcg accttgaccg
     8281 ctggggtgtt cgcgaaaaca ctaaccgtgg agcggatgcg gtccgaagtc cccgcgattc
     8341 aaaagaccgg cttggtactc tcgggaggag gcacccgcgg cgcgtacgag gtcggtgtgg
     8401 tctccggtat catgcaggta ctgaagccca agctcgggga tccgcccgtt tttcggatat
     8461 tctcgggcgc ctcggtcggg gccatcaaca cgacttttct cgccgccaac gcggagctgc
     8521 ctgaccatgg cgtcagcaag ctcagccagc tctggaaatc gctcgatctg agcgagcacg
     8581 ctcgaatccg ccccctcggg ttggctcgat taccggggag cttgaggagt cgactcaaga
     8641 gtctggccgg ggagaagctc ggcacctcgt tgctcgatac gcgcgcaatc gatctgctcg
     8701 ttcggcgcag catcgattgg gacaagctac atcgtgtgat cgacagcgga cgcgtgctct
     8761 cgttgctcgt cgccgcgctt cacgtcgctt cgggccgcac gatcatgttc agcgagcatg
     8821 cccccgggac tacggtgatt ccgacccggg acgagcggcg cgctaccgtg ttcgagcgca
     8881 tcacgcccag tcacgtgctg gcgtccgcag ccattccact gttgttcccc tcgcagcgca
     8941 tcgggaaaca ctattattgc gacggcgggc tacgcttcaa cacgccgatc gcgcccgcca
     9001 tccgtgccgg ctccgatcga ctcatcatcg tgagcgtgcg acacagccgc acggcgcgag
     9061 acatggccgc cgtggaagag gcagacacgg gcgaaggtcg cgatttgggt cccgcgtttc
     9121 tcatcggcaa gctgttgaat gcgttgctgc tcgatccggt cgcctatgac cttcaagtcc
     9181 tcgagcgcct caatcaggtc atggatgtgc tggagcaaag cctgtcacag gccgagctgg
     9241 atcgcgtgca gcgcgtttgg atcgagcatc ggggcctgcc gtatcgaaag ctgaagactt
     9301 tggtcttcac cccgtcacgc gatttgggtg aaatcgcggg cgactacttg cgcacgcgga
     9361 tgcgaacgga tcatttgaga ccgctcgctc gctacttgat ggaccgcgca gttcaagcga
     9421 gcccgcacgg ggaagcggat tgggcttcgt atatcttgtt cgacggcagt ttcgcgacgg
     9481 agctcatcgc gatcggtgaa cgagatgccc tggagcgacg cgacgaaatc ttggagttct
     9541 tcgggcgaac gggtcagccc tgatctgggg ccatccggct agagcaccgc gcactcgggt
     9601 ggcagcactg gatatgcgcg atccaaggcc gtaaaacacg cccgcacgaa cttcacggag
     9661 tgtgccgctt ggtatatagg gtgcatcgct gagggatcgc tctccgcgat cgagaggatg
     9721 atgcacgtga acaaagcacg attgcacccg ggtttcttgc tgggatttgt gctcggtacg
     9781 ctcagcggcg cagctgcgtg cgggagcagc tccggcggcg agctgttcag tgccggaaac
     9841 aatccggcgg gtggaacccg ctccgtaggc accccttcag gcccgcaaac gagcggtgtt
     9901 ttcggggccg gaggcacagc caatgcgagc ggcggtagcg catcgggtag ttcgagcgtc
     9961 aacgcttcga gtgcgggtgg cgccaactct tcaggtggtt cgaacttcaa tgcgagcggc
    10021 agtggcggcg caacggacgc cgcggggaac gcgggaactt cttcgagcgg tggttcgagc
    10081 ggcggtgcga ccgcgcaagc cggaacggga ggtgctccgg cgggcggatc ggcggcgctg
    10141 ggcggcgctt cctctgctgg cgcggcagtc gggggcacat caaccggcgg tggagctgca
    10201 ggcgctacga tgacgaacgc gggctcgagc gccgtgggcg tctgcgccac gttctcgagc
    10261 gacatcgcga cgctcacgcc aacgctgcag gcggcgcaga cttgcgatcc caaggtgatg
    10321 aatgcctgcg ccgtactgga acagggggac tgcggttgcc cgatccaggc tcaggccaac
    10381 cgacagctat tggagccctt gcgcgtgcga ctccaagatc gccccgtggg ctgccgctgc
    10441 ccagaaatct gcccttcgag tcagggcagc ggctcgcgcg gcacgctctc ttgcatgcct
    10501 gctccgagtg gcctcggaag ctgtgtattc ctgaaatgag cgccgcggcg cggtcaacag
    10561 cgtgccggtt gaatcgcgac ggtacggagc tgttctcggt gaaaggccgt tccaaacact
    10621 gttttgccct gcgccttgaa ccgaacttcg tagagccccg ggcgtagcgt ctccagacgc
    10681 gccgtgaagc ggccttcgat gtcttcactg agcttgagct cgctgcacac accctccgga
    10741 tagcgcacct cggcctggac ccgtgcgcgc tcggtgatcg gcaacccggc ttcgaacagc
    10801 acggccgtga gcagcaattc gccgccaacg cgcggcgaac tcgcatgcac ctgcgcgtcc
    10861 aagaccaggt cgctcttggc aatgacgaga taagaaaacg gcgtcgtgga aggcttctcg
    10921 tctttgccga tgcgttgcag gcgaacgctc cgggaagccg gtcgcaaacc cgccggatcg
    10981 gggtcgttgc tcaggcgagt gagcatgaca ccgtcgctgc gctctaccga ttgtactttg
    11041 aggctgcgcg gggactcgta gctgcgcgcc ggcccggtcg acagatctgc agcggttgcg
    11101 gtttctgcct tggagtgcgg cgaaggtgcc gtgatcggtt cgagagtcaa ccgctctggt
    11161 tccttcgcga acacgacgac tttgaattcc tcatcgagtt ccgtcgtcga gaactcgagg
    11221 tcaaaaaaag cttggggcga cgccggcaac gcctggtccg gatcgacgac gatggtcgta
    11281 ttggacacgt tggcgaagat ctgcgccgcg tacttctgta ccttgaacag cacgtcaccg
    11341 gttacggcac cgtccaccga gagatagccg ttggcggagg tgagccaact cagcgacgtg
    11401 gtgttgaggg atggcactcc gatcgcgtac gtgtccggca attgcggcaa cttgctcacg
    11461 gaattgccgt agtcctcgag accgtccgtc agcacgatca agttcttgtc tgcgccgggc
    11521 gagaggtaga gcatgatgcg gcccttctgc acgcctgcgt agatgcacgt gggctgcccg
    11581 ggcggcgtca agaaggcggg attcaggttc acgggatcga gcagggtttg cgccacctgc
    11641 cgccgcgtgg acgtgccgat catctgattg agcgtaccaa tgctcaacag ctcggtcgcc
    11701 tccgccgcaa aactcacgat acccaggcga tccgtatcct ccatgagagc gagcagggcc
    11761 gtcgcggccg agagcgcccg acgaacccgc gattcgccgg cgaggagctc ccccatgctg
    11821 gtcgaacgat cgatgacgag cgccagggtc accgtttgcg cggcggaaat ggagccactg
    11881 acgttgagca cccgttccga gtcaggcagg gggttaccgg cctcgtccac caccgagagc
    11941 tgcagtgatc ccgccaaagg ccccaacacc aaggactccg acagactcac caacaggtaa
    12001 accgtgggga ggagtgctcc gccgattctc accaacgggg attgcacggc attcgtacct
    12061 acggcttccc atcccagcgc cgcgagcggc acgaactgat cgggcgtggt cacaggacgc
    12121 gagaccttca cccgaacatt gcggccggcg ctgaaggggt cccccaccgt aacagcgact
    12181 ctcacgtact tcttgcgacg cgccggaacc gcgccgaaat cacaggaatt cggagagaaa
    12241 ttgacctcga tactcatggt ttcctccaag gcggcccgag cccacccatc ggggaatggc
    12301 cggcgcgccc acgatcggtg agcttccgat agagctcgcc caaagtgtca agttgctcaa
    12361 cccgtgtcaa ttacggtcta ttgcagctcg aatccgtcag gcgccgcgtt acgtattgaa
    12421 gctcatatac gggcaaccaa gcggcgtcgg tcgcgcatgg cctcgtaatc catgcgcacg
    12481 acaaagcgcg tgactcgcgg gtcaaagcca aggcgcggac tgcaggtatg taactgagcc
    12541 gcaacggcgc ccgagggtat cgataggccg cgaaggaaag tgatcttagc taggggcggg
    12601 ccctcggagc gatgggcgct cgaaaacaga ggtgtcagaa acttgagtcg gcggcggcag
    12661 gcgcggcacg atcccgctcg atgccgtgga ggtcgttcgc ctgggcccaa cccttcctcg
    12721 ttttggcctg tctattggct ggttgcgcac gcgaagctcc caccgccaag gccccggagc
    12781 cggagttcac cccgtgggtg acggcggcag actacgcttg gccgcccaaa actgtgaccg
    12841 cgacagatag aatgcacccc gatcgaaggg cggccgcgct atcgagcgtt gacccaatta
    12901 ccacgccaca acggcccccc ttgccttcag acacactgat cccgggcggg caggcgtgcc
    12961 tcgatcagct cagcgcgcaa aatgtcaagt tcgaggtgct cgaagccgag cgaggagtgg
    13021 acactcccgt gctgatccgg ggcgcactcg ggggagtaga gttctggtct acgggcgggc
    13081 caatgatcgt ggactgtcgc atggctctcg cgctgctgca tgtagcgccc gagttcgtag
    13141 ctcttggtat tcagcgcgcg cgattctccg gggcgtacgt gtaccggacc agcaaaaagg
    13201 gacggctcag cctgcacgcc tacggactag cgatcgatat gcacgacttg tctacggctg
    13261 aacgaaccta ttcggtaaag aaggactatc agcgtggcct cggcttcgcg tgctcacagg
    13321 gcgcccccgt gctgaacgcg ctgagttgcc gcttgaagag agacggctta tttcgagagc
    13381 tgctcacgcc cgactacgac gccgatcact acgatcacct gcacctcggg ctagcgccgc
    13441 tgccccacac gctggcagac cccagagctc gcgtctcgct ccccgaggtc gtggagcatc
    13501 cggcccccgc gcgcgcaaca ccctcggtca cccgatcgac ctcaaagacc aaaccgatga
    13561 cgcacgatgc ggtgcgtgaa gaccggccgg agataaaacc cccgcggcag agtgcgaagc
    13621 ggcgccccat cgttgtcact ccccaaaaag cgcacgctcg cgtcgcgcgc cttcggccga
    13681 atctgaagga actgccccac gtgacccgtg acagcctcga ctccacgccg agccactagc
    13741 ccggcgagtt cgtcccagtc gaaacgcagc gccgagccct catccgcgtc cgggctccaa
    13801 atgaagggcg taccgatccg gcgttgattg agcggaatgc gccggtcccc ttggaccggg
    13861 acccaaagca cccgggtgag cttgcggcag ggccgtgact gctcccactc gagctcggtc
    13921 agctccgaaa gcaaaatggt ggccacgaac gtcgactcga acgggcgccc cgccgcatcg
    13981 accggcaagg tcttgagctc caccccgagc gcctcgaaat ctggggcgtc gcgcgacccg
    14041 gcggacgcac ccaaggcccg ctcgatcagg tgcccaaccc accccttgtg atgattgagg
    14101 tttgcaggca ctgcgtgtcc cagacgctct gcgactccgg agagcgagtc accggcgagt
    14161 cgctccgctc gctcgagcag ctcggcttcg gtttggggtg tcgggattcg gctcaattcg
    14221 gagcatggag tatcgcggtt ctaaaaacga tcaacgccaa tcattagggg gcacgcggga
    14281 gcggtgctaa cttgcggagc gtgcgaaaga ctgttgcgct cgtcttgtgt tcgctcttcc
    14341 tagcgtgcca agcgacccgc gatctcggat tggcgaccga agcgcgctcc gagctccgca
    14401 gtcaaaccga gctccgcagt caagccgagc tccgcagcca aaccgagctc cgcagccaaa
    14461 ccgagcgcag tgcaccagcc cacgtccccg tgagcctgcc cgccccgagc ggcgcggtcg
    14521 ctctgccggc ctcagcgtta ccaggcactg cgccccacag ccctcccgcc agcgagccgc
    14581 cgcggcttta cgcaaaaacc cgcttcgtgt gggtatatcc ccggccagac gcgcaggccc
    14641 agtggctcgg ctttctacgc agcggcagct ccgtggaatt acgcggaacc aagccccaag
    14701 caggtccggg ctgcgccgcg ttctatccca ttgcccctga gggcttcgtc tgcggtgagg
    14761 gcaatcgcgc aacgctagat catgatgacc ccgtgtaccg cgcgctcgaa ccctacgcgc
    14821 tgcgctcgga taaggcttat ctgcaccagt acggcgaatc actcggcctg ccgcgctatc
    14881 attccctgcc cgacgagcgc gcacagcaag aacgcgagac ggatcttcga tcccacctga
    14941 agaacgtgga gcgggcacga caaggatccg tgccgaccgt tttgacgggc gtagacgtga
    15001 gcttgcccgc gagcacgctt ccaggctttc ctacgctccc ctggcccgtg ttcgaggatc
    15061 ggaaagatct gaagcctcgc tcgacggtcg ccttccacac cgaaacgcgc gttggcgatc
    15121 gtggattctt gctgacggga gactttgctt gggtgcccaa ggaccgcgtg cgtccctatc
    15181 ccaagatcga gtttcatggg gtcgagctgg ctgagcttcg gcttcccttg gccgtgttca
    15241 gaaaagaagg cacgctcaag tacgcgcgcg acgcaggtgg ctcgtttcac gcgctcggcg
    15301 atacaccgga gaaccacttg gcgcggctct cgttcgtgcc gctgacaggc aagctggaga
    15361 gccaagccga cactcagtac ctcgaagtgg tcggcggcga gtactggatt cgccagagcg
    15421 acgccgtagt gcctactccg cgcgagacca ccccttgggg cactcgcctt gcggatccga
    15481 acccgcccgc tccccccaag ggcggccgtg gcacgtgggt cgaggtctcc atcgatcagg
    15541 gttggctgat cgccttcgag aacactcgcc ccgtcttcgt caccctgatt tctccaggtc
    15601 gcggaggcgc cgctcggccg ggcgaggatc cgctcgagcg cagcgccacg ccgacgggca
    15661 cctatcccat cacggggaag ttcctgacgt cgacgatgga ggcacccggc gagttcgttc
    15721 actcggacgt gcctttcgct cagaatctga ccggtccgta cgcgctgcac gccgcctact
    15781 ggcacgacaa ctggggaaac cctcagagcg gtggttgcgt gaacctgtcc ccactcgatg
    15841 ccaagtggtt gttcgacttc accgagccga gcctaccgaa gggttggcac ggcgtgcgtt
    15901 ggatccccca gaacggccct gccaccatcg tcattttgca ccgttgagtt gctggctcga
    15961 gtgcagccct cgccgcgcta tctatcgacg acggcgcttc ttaccgcccg atcgtcgact
    16021 cggcgcggga gcgtcggaga acatctcgaa aatcagctcg gccaactcct ccggaatctc
    16081 gtcggcctgc tcttcttccc gcagcgctgg cggccccgcc atgaattcat gcaaagacat
    16141 ccggcgctcg ctgaacgcca gcacgtactc gtgaatctca cgcagcaacc cgagctcgcg
    16201 ttccgctaac tcgagcgctt ctgcagccga aatgcctttg cgaccgcccg caaacacgaa
    16261 ctcgcgcgac gcacgacgca actcgcgtac ctgcttcgag agctctcgaa gctgcgtttt
    16321 cagctcctcg atcagcttat tgagccgctg cgcacgaacc tcgctggctg ggcccgccgt
    16381 gacgtcgggc ccgccgttca ccaggctagc ctcaatctgc atcaaccgcg caaggtcgcc
    16441 ttcttcgtag gcgcgcgtga cctctttcat gatgcgggtc cgttcttcgc gttcccgctc
    16501 gtcccgcgta cgatccggat gtagcgcgac ggcgagcttc cgaaacaagt ccttcaaccc
    16561 ggagttgccg ggccgagctc cggcgggtga cgcggagccc acccgctgct ccgggccagg
    16621 cgccgctccg ccacgcctac cggttcgggg tggtgcctgc tccgcgtcga actccgcatc
    16681 gaaatcatcc gagggctcat ggaacgactc gtcgtccgac tcgtcctcgt ccgagccctc
    16741 ttccggcgcc aagcgaccac tgacccgcag cgagtggtaa acctgacgaa cggcgcgttg
    16801 cgccgctcgc ggcagatcgt cccgcctgag cagcgccgag aatagcgcgt ggatttcctg
    16861 actcaaacgg accgtctgtg tcatcaacgc tgggaaacgc gagcgcacca gctctgcgga
    16921 tgcggcgacc gcttgctccg ctttttccgc ctctcgccgc ttgcgcccga tcttcttcag
    16981 caagtcgtcg cgagcacgga ttagctccgc ctcgcgcaat aagtcattgc cctgaccgag
    17041 cgccgtagct tccggtttct gcttggcacg agcgcgtgtc atcgctgcac tatagcgtga
    17101 agcatgtttc gtgccgcttc gctcacacac tcgaagggta gacgattcac ggccgcgacg
    17161 tggcctccaa cgccgcgctg agttcgccaa gctcccgctc gaagccaccc gagaggatcg
    17221 gaaatcgata ccacgtcttc gggtccaagc tctcgtcgtc gaggtggaag gagggcgcat
    17281 aacgttcacg cttccactga ttccggcaaa atagcctgaa gaaccgcgtg atccaggcaa
    17341 ccagcagggt gtcggcgcgc tcggggaagt ccagacgcaa ggcctccaaa atctcgctgg
    17401 gtgagcgctt gtcccgaatg gcggcccgtt cgatcgcgtc gagcacgtcg tacggcatca
    17461 gatctccttc gtccgtttga ccctcaccgg gcggtcgcag ctcagccgtc ggactctgtt
    17521 gattgacggc ctgcaaggcc ggaatcggcg cgagcccact caacccctct gactcgagcc
    17581 aaaccaacca acgacgtagg aaagctttat cgatgccggc gatcgggctg atactgcccg
    17641 ccgtatcgcc atccatggtc gcataaccga ccgcggcctc cgagcggttg ctcgtggaga
    17701 gcagcagcgc cccttgcacg ttggccacca accacgcgct cggggcccgc acgcgtgctt
    17761 ggatgttctg caaagcaata tcgtcggtct cccaagccag tgaacgacca aataccttct
    17821 cgcccaaggc cacgtagcct tgcacgaggg agtccacctc cagttcgaag tgccgcgccc
    17881 ccaccgcctc cgccaacccg ctcgcagccg ctcgtgtcac cggcccgctg ttacgcgtag
    17941 attgatacag cgtcgtgagc aagcgcccta cgatctcgcg cgtgcttcgg cacgcctcca
    18001 agccgcgcag gtaaccgagc cgcgcctgca cccctgcgac ccccaagtcc ttgcacgcca
    18061 actcgaccat cgacgccacc aaacacgtga tcgcggaaga gtccgctccg ccactcaagg
    18121 agacgacgaa accgcgcgaa cggctcttgc gcagatagtc gaacagcgcg agcgacacgg
    18181 ctcgtccaaa ctcttcctca cggatccgag gtgaaccttc ccaggcctca ccctcgatct
    18241 cgttgcgttc gagtcgacac gccgggtaat cgaacggcac cgcgacgcgc gatatattct
    18301 tcgacagcgc gggcctgaag ctcgtcagtc gggtgtgatg cgtgcgcgtc aactccacgt
    18361 cgatcacggc gctcacgagc tcgtaagggc gatagctgaa acggcgtgac tcggcgcaga
    18421 tcgctccggc cgtcgcgatc agagcatcac catcgtagat cgcgcgccct gcctcgttgc
    18481 cgagcaggtt cgcataaagg taactgacgc cgaacgcgcg cgagccttcg agcacgagcc
    18541 gacggcgcac ctcgtgtttg ccgaacgcaa aatggctggc gctcgggttc aagatcagat
    18601 cgaccccatg gcgcgcgagg cgcccgcccg gtcgctccgc aatccaagcg tcctcgcaga
    18661 tctcgaaacc gaggcgcacc ccacccacgt cgaacagcac atccccgacg cgcgcctcgg
    18721 tgtccaatac ttggcacgtc gccacctttc ctcggggcca ggccttgaac caacgcggct
    18781 cgtaatggag cccgtccccc gccagcgctc gcttcgccac gaagccgcag agcttgccat
    18841 cggcgagcag cgcgcaggca ttgaagaccg ccttgtcgag ccgcaacggc aacccgacgc
    18901 agacgacgat cccccgactc tcacgctgca cttcagccag cacctcgagg gcggtgcgct
    18961 gggtacccgg cgccaggaac gcatcctcac agccgtagcc ggtgatgcaa agctccggca
    19021 agcaaagcac ggagacgccc tgtttccgcg cttcggccag caccgcgaga atgttcgcgc
    19081 agttacctgc ccaatccaag ggggtttgat tcagtgcggc ggcggcgacc ttgatcagct
    19141 tcatgcgacg aaccagcgcc gcgagcgggg cgcccgcggc gaccgcagtt tagccggcgc
    19201 ggagtcccgc gaaaggatcc atgcgtcgaa acctgatctg ccgcggcacg gtctggcttg
    19261 tcgacgtgcg cccgcttgcg gccaagcccg aggccgcgcg tcaagtcgcc ggtggagctc
    19321 tcctcgcgag caactaggcg gatcggtcgg acgtcggcgg ctccgcgctg cccgcgtcgt
    19381 agaccacgac ccgattgcgc ccgagacgct tggcctcgta caaagccacg tccgcggcct
    19441 tcaccaggtc ttcggccttg ccggaagcca cgtcggtgtt gccggccacg ccgatgctca
    19501 ccgtcacccg ttccggtttg ccgtcgaacg tgagctgaat gctctcgatc gcgcggcgta
    19561 ggcgctcggc cacgatccgc gcatcctctt tgcgcgtgtg cggcagcaag accgcgaact
    19621 cctcgccgcc gtagcgcgca gggacgtcca cctcgcgcaa attctcggcg accttctgag
    19681 cgagttcctt cagcacgcga tcgccagcgt cgtgcccata gtgatcgttg atgcgcttga
    19741 agtgatccaa atcgagcaac aagcagctca aagtattctg atagcggcgg gtgacggcgt
    19801 actcgcgctt caagacgtcg gcgaagcggc ggcggttgaa cagcccggtc agcggatccg
    19861 taatcgccag cgcctctacg tgatgcagca tggcttcgag ctcttgattg cgctcgcgca
    19921 gctcgagatt ggcggccttt gcacgcagcg acgcgaagat acgcgcctcg agctcttcgg
    19981 tctcgaacgg tttggccaag tagtcatcgg cgccgacgtt gagcccctcc acgcgatcgg
    20041 cgagcgcggt tcgaaccgtg agcatgatga tcggaatgtc gcgcgtgtcc gcgtgcatct
    20101 tcaaccaacg acacacggcg aacccatcga tgtcggccat caccacgtcc agcaacacca
    20161 ggtccggctt ttcggtgcgc gccaagcgga gcccttccac tcccgaacgg gcccactcca
    20221 cggagtagcc caggtgctcg agcgagctcc ggatgtggtt accttgagct tcgctgtcat
    20281 cgatcagtag gagcttggct ttcaccaaca attccctgtc gttgtagata ctcgatcagt
    20341 gtgccccgga acggccgact tcgaaaccgt atttacccac caaatcacga tctccggtaa
    20401 cggcccctgg ccacttgccc gaaagcaagg gctggtatgt gggttcgagc cccagccgcg
    20461 gggccaccga gaaaataagc ctcgaaacgc cctgagcggg acccgctcgg tcgcgcccac
    20521 actgggcgaa acccacgcat actaaggcag atatcggacc ggcggctcta aactgggcac
    20581 cgaatcagcg cgctccagcc gcgctgcccg tcggaatgct agacgtctgc ccaaagctag
    20641 gtcgcgcgtg cgacttggcc tgcgcaagtg cgcggctgag ctcacctccg cgcaagcacg
    20701 agtaacagtc ccccaacccc aagatgccgc agccttgtcg ctccgtgagg cgattcagta
    20761 acggcaccga tcgctcgtcg aagtcctgct cgcgctcggc gagcaaccgc ttcaagtctc
    20821 cgcagcgcgg cttcttgagc gccaactgca gcgcccaaaa tgctttcaag ttgggagagg
    20881 cttggctgcg cgccggctcc acctcgagca acgccttggc ctgttgagat tgcccggtgt
    20941 tactagcttt accgcgctcc caaacgtcgt acagcaagtc ggcgccggcc gggccgagcg
    21001 aacgagctgc caactgcagc acctgcccgc ccacgtcggg gcgatcggca agcgcgcgca
    21061 agtcagccag cagcaacggt tccttggcga gctcggggcg ctgcgatagg gccgccgagt
    21121 aggagcgcaa gcagttcttt gcatctccca gcctgcaata gccgcggccg agcgctcgcc
    21181 actccacgag cgagcggtgt tcggcagagt ttgcggtttc gagctccgcg atcgcctttg
    21241 cgtcacctgt ttccgcgcgc gtcacgagcg cgagcacatt caccggtggc gctgccgcag
    21301 cctcggcctt gggcgcttcc gcgtgggcgg agggcgccgc gctcggaccc atggtgagca
    21361 aagtcgtggc gagcccggcg accaccaaca ccaaaccaga gatcagcacc agcagcgaga
    21421 ggggcaacgt gtaacgcccg atcgaaagcc ttcgctcgag ccgcaaagcc gcaagcttat
    21481 gctggcccca agccaaagaa gcgcgcagcg ccgactgcag ctgggcccgc gaaaccacat
    21541 cgcgcacgtc cgccaaagcc aggccgaagg cacgacccgc agcggcgttg ccgggagcgg
    21601 cggtcgcgag cgccggcgcg gtgatcgccg tcaccgcacc gctgctggaa ggaaactcgg
    21661 cgcgccccag cgccagtagc tcgtccagtc gctctcgcac caccaccgcg gtctgtggac
    21721 gttcgtcggg gttcttccgc agcaactcga acacgaagcg cgccacggga gctggcaccg
    21781 tctccggtag cggatcgggc tcggccgtga tctggcgcat caagaccgcc accaaatcct
    21841 cgtgccggaa cggcgatttg ccgctcagca tctcgtacat aatcacccct aaggtataaa
    21901 gatcggcgcg cccatccacc tctgccccgc gcgcttgctc cggtgacata tactccggcg
    21961 tcccgaacac cattccgagc tgggtcagct ggcgatcccc gccccgcgac tccaccggca
    22021 ctttggcgat cccgaaatcg agcaccttga cgaagtccgg caccccatcc cggttcgtca
    22081 acatgatgtt ttcgggcttc aaatcgcgat gcacgatgcc cgccgcgtgc gccgcggaaa
    22141 gcgcgtccgc gatttgacgt gcgatcgtca aaacgcgcgt ccgcggcaac gttccttgat
    22201 ccttgagcag cgatgccagg ctctgccctt cgatcagctc gagcaccaga taaaatgagc
    22261 catctgacag ccgcccgaag tcggaggcag acgctacgtg ctggtgctgg atgcgccccg
    22321 ccgccaccgc ctcgcgctcg aagcgctcga cgatctccgg catctgcgtc atcgagcggt
    22381 gcagcacctt gatcgcgaag gccttgcgca tgtgcacgtg ctcgacgcgg tacacggtgc
    22441 ccatgccccc ttcaccaagc agcgcctcga cgcggtatcg gtcaaccaac acacgtccga
    22501 tgaggctttc agaagccacc tcactgtcgg gcggggtgct cacggaatcc gtatcttgcg
    22561 ggcaagctgg gcccgcgtcg agccattggt cccaggaatg taacgcgaaa gctctaggcc
    22621 caaagcgccc cctcaactcc taccattcag caaaccgacc ggcctagcgc tggcactgcc
    22681 gttcgtacca ccactcgttg cgttttcaga gcgaaggccg cggtgcagca cgatcttctg
    22741 accggcgctc cgtccttccc cataagcggc gtgtgaggcc tggctgcggt agtgggtgtg
    22801 tcggattctg gggtaacggc tgcggaagta acgttccagc ttcgggtctc cggcatacac
    22861 caagccttgc tgcttctggg tgtgccgctc cttctccagc ttgtcccgaa acccactcat
    22921 cacgccggcc acgaagctac ggcgtccgcc gttaccctga aggccgcgag atcgcctgtg
    22981 ggtctgccag aggtgctcgg acgtgtgcat caggaaagcg tgcacatagg ccgcaagttc
    23041 cacattctcc aggctgccac agacctcgag cacgctgccc cggcgccctt cgagcggtcg
    23101 atacaccgga acccaaatca cctcgacgaa aaagaagtcg ccgagtaacg ccgccaagat
    23161 acgctcggct tcactcacgc gaccagtagg caccccgagg tgccgatagg tatactgccg
    23221 ctcacccggc tccgaaacac tatcgatatt gtatttgagc atcagccgct gtgctgcgct
    23281 catcgctgcc tgagcttcgt gctcgttttg gctctgcgcg agcatcagca gcttggcgat
    23341 acgatccagc acacgcgcgc gcgcatccga cgtgggctcg gcatccggca agccgtgggc
    23401 cttggcgtcg aacccgcgcg cacggcagac ttcctggaaa ttcggcccgt gactgggctc
    23461 gtcccgaacc cccaacacct cgtccacgta ctggtgcgcc atctcgtgct tgagcacctc
    23521 ggtgagcacg ccccagccat gctccaagag caaggctcgc gaaagctcga tgacccgcgt
    23581 cgagctcacc caacgcccga ggtgcgctcg cgtgtccgag agctcgaaag ccggcgcgcg
    23641 caactcccaa cgaaagaagc agccgttcaa atcgtcgaag gtccgacgaa tcgcgtgtag
    23701 cgctgcgcgc tccagctcga gggtcaggcg agtggcgtta tccatcgggg ggaaagcata
    23761 tcgcttcggc cggcgaagtt gtacgatgaa gccatgtcca aagacgagga agcgctgctt
    23821 taccactcgg aggggcggcc cgggaaaatc gaggtggttt ggaccaagcc ggtggcgtcg
    23881 cagctcgacc tctccaaggc atattcccct ggagttgcgg cgccatgtcg cgccatcgcg
    23941 gctgacccgg acaaggtgga tctctacacc gcgcgccgca acctggttgc ggtggtcacc
    24001 aacggcaccg cggtgctcgg gctcggcaac attgggccgc gcgccgccaa gcccgtgatg
    24061 gagggcaagg gcgtgctgtt caagcgcttc gcggacatcg acgtgttcga catcgagctc
    24121 gacgcgcaag acccagaaga agtcgtgcgc atcgtgcgcg cgctggagcc cacgttcggc
    24181 ggcatcaacc tcgaagacat tcgggcgccg gactgcttcg tcatcgagcg cgcgctgcgc
    24241 gagcagatgt ccattcccgt atttcacgac gaccaacacg gcaccgcgat tatctcggcc
    24301 gccgccgtgc gaaacgccgc ttacctccag gaaaagaacc tcgccgattt gaaggtcacc
    24361 tgcgtgggtg cgggggcggc cgcgaccagc tgcatgcgca tgtgggtgcg agcgggcgtg
    24421 cggcgcgaga acatcaccat gctcgacgtg ggcggcgtgc tccacctcgg gcggccggac
    24481 ttggatcaat tccggcgaga cttcgcgcgc cctctcagcg atccgcgccg cacgctccaa
    24541 gaagcgatcg tcgacgccga cgtgttgatt ggcctctccg tcggcggcct tgtcacgccg
    24601 gccatgctca agaccatggc caagcgaccg atcgtgttcg cgcttgccaa cccagatccg
    24661 gaaattggtt atcgcgaagc gatcgccgcg cggcccgacg ccatcgtcgc caccggacgc
    24721 agcgacttcc ccaatcaggt caataacgta ctcggcttcc cctacatctt ccgcggggcc
    24781 ttggatgtcg gagcccgtgc catcacggaa gaaatgaagg tcgccgctgc cgaagccttg
    24841 gccgagctcg cgcgtgaaga ggtctcggac gaggtgttgc gcgcttacgg ccggagccgc
    24901 atgcgcttcg gtcgtgacta catcatcccg aagccattcg atacgcgggt gctgtattgg
    24961 gtggctcccg ccgtggcgcg cgcggcccaa gagagcggcg tcgcgcgctt cagcatcgac
    25021 gtggacgact atcgtcgccg cctattccag cgcatctccc ccacccgccg cgtgctctgg
    25081 aacatcctcg agagcgcgcg ctcctcaccg cgccgcatcg tctacccgga gagcgagcac
    25141 gacggcatcc tgcgcgccgc gtaccgcgtc atggaggagg gcattgccca tcccatcctg
    25201 atcggggcgc gtgatcgcat cctcgagcaa gcctctcact tgggcgtcga tctgtcgcgc
    25261 gccaccttgg tggacccacg cgagtcagac aagctcgaga gttacgcgga cgccttctgg
    25321 gccaagcgcc agcgcaaggg cctcacccga cacggcgccc tgaaagaact acgccgttca
    25381 cgcaccttgt tcggcatggt gatggtcgac caaggtgacg cagacggcat tgtcgccggg
    25441 gctcgtcaag actaccccgc gaccatccgc cccgcgctgc aagtcatcgg cgtacgcgac
    25501 ggcgtcacgc atgcggctgg catctacatg gtggtcagca agtcgggcgt gcgcttcatg
    25561 gccgacgcca ccatcaatat cgacccggac gccgacacct tggccgagat cgccatcctc
    25621 gccgcagaaa ccgtcgcgga cctagggctc gaaccgcggg tggcgctgat cagcttctcg
    25681 aacttcggag atgcaccgca cccgtcctcc aagaaggtgg ctgaagccgt ggccatcgtg
    25741 aagcgtcgcc gtccagactt gatggtggac ggcgaaatgc aggtcaacgt tgcgttggac
    25801 gaagaggccc gcgcccccta ccccttcagc aaactcaagg gctccgccaa cgtgttgatc
    25861 ttcccgaacc tggacgccgg caacgccgcc tacaagatcg tcgcggccag cggcgaagcc
    25921 gacgtcgtgg gcccgatcgt catgggcatg cgccgccccg tcaacgtgct ccaacaaggg
    25981 gcgagcgtcg atgccatcgt gcacatgacc gcgattaccg ttgcgcgagc caaccgtctc
    26041 gacaaagatt cgcgctaacc gatgcagctc tcgaggctcg tgcccgcctt gcttgtccta
    26101 gcgtcgggca ccggttgcgg aaaattcaaa cgcagcaaag aatgcggcgc cgtcgcgggc
    26161 gcggtgtcta ctttcgtttc tgcgtccgct cccacgccca tcggcaaagg gcagcccgcg
    26221 tccataacgc ctacggactt ggcgcgcgat gcacgggcca ccgcccgccg ttataacgaa
    26281 ctggaccaaa ggctcgaatc gttgcacgtc cagtcggcag atctggcgcc gcgagtcacg
    26341 cattatcgtg agctcgcgcg ccaagccgcc atcgcgctcg aagacgccgc cactgcgctc
    26401 gaacacagcg acgcggagac cgcccgcaga aagcgggtcg agttcgacgc cgtcgcgcgc
    26461 tccgagcccg cgctagtcgc cgagatcaac aacatctgcc gtcgttgagc ttggcattat
    26521 ggtggccgcg ttcgaacgag cgcgacctcc agtcggggct tcacgagagc gcagccccct
    26581 tgcgctctcg ctcccaccca tcaccgcgcc ggcccccacc gcaacgcgct catccggcgg
    26641 cagtttcgcg cttacggccg gaacgacgct cgactcagcg tctgcgacgg cccgcggccg
    26701 cgcccttgcg ggcgccgcct ttggccgccg ctttcgcccg agccggtgcc atcggtgccg
    26761 ccgccggcgg caccacgtcc ggatttcccg ccgacagccg acgtaactcg tcacggagcc
    26821 gagccgcggt ctcgaactcg aggttctccg cggccgcgaa cattttctgt cgcagctcga
    26881 ggatgcgctc ggcaagattc tcgtcgttcg tagccgactt ggacgcgccg ctctcggccg
    26941 gcgcgttcgc cttcttggac gatccgcggg gcaccgcgta gtagtccgac tcgccactcg
    27001 acggcgtcat ctcgaggatt gcgcgccgca ccgtctccgg agtgatgccg tgttcgcgat
    27061 tgtacgattg ctgcagctcg cgccgccgct cggtctcgct gatggcaatc ttcatcgcgt
    27121 ccgtgatgcg atccgcgtac atgaagactt ggccacgtga gttacgcgcg gcgcgaccga
    27181 tcgtctgaat cagcgaccgc ggactgcgca gaaagccttc tttatcggcg tccaagatcg
    27241 cgaccaagct cacctcgggc aaatccaagc cctcgcgcag caagttgatg cccactagca
    27301 cgtcgaactc gcccgcccgc aaatcgcgca agatttcgac acgctccaac gtatcgatat
    27361 cggagtgcag atagcggacc ttgatcccga gctccgagta atactcggtg agctcctcgg
    27421 ccatgcgctt ggtgagcgtg gtgaccagca cccggtcccc cgccgcgatg cgcgtcttga
    27481 tttcgccgaa caaatcatcc acttgcccgg cgacgggccg cacctggatc tccggatcca
    27541 ggagcccggt gggacgaatc acttgctcga tgatccgccc atcgctctcc ttgagctcgt
    27601 agtcgccggg tgttgccgaa acgaacacca cgttgcgcat gtgcttttcg aactcttcga
    27661 agcgcaaagg gcgattgtcg agcgcactcg gcagccgaaa gccgtactcc acgagcgttt
    27721 ctttgcgcga gcggtcgccg cggtacatgg ccccgatctg gggcaccgtc tggtgcgact
    27781 cgtcgatgac gagcagaaag tcctcgggga aatagtccac gagcgtgggc ggcggatccc
    27841 cgggagcgcg accggacagg tggcgtgaat agttctcgat gccctgcacg aaccccattt
    27901 gctcgagcat ctcgagatcg tagagcgtgc gctgccccag ccgctgcttc tcgagaaacc
    27961 gcccttcacg gtcgaagaac tcgagccgct ctttgagctc ctcgcgaatg ccctggatcg
    28021 agcgctgcag ctgctgacgc ggagtcacgt agtgggagcc ggggaacact gcgtacttat
    28081 cgagcgaacg caggatgcga ccacgcagcg gatccacctc actgatggat tccacttcgt
    28141 ccccgaagaa ctcgacccgc accgcgagcg cttcctcgta aaccggaaag atctcgacca
    28201 cgtctccacg cacccggaag gtgccgcggt ggaagtccgt atcgttgcgc tcgtattgaa
    28261 tgtcgaccaa acggcgcaac agctcgtctc gccggagctc ctcacctttg acgatctcga
    28321 tcagcagacc cgagtaggtc tccgccgaac cgataccgta gatgcagctg acgctcgcga
    28381 cgatgatcac gtccgatcgc gacagcaacg actgcgttgc atggtgccgc atgcgatcga
    28441 tctgatcgtt gatgatcgca tccttctcga tgaaggtatc ggtggtcgga atatacgctt
    28501 ccggctggta atagtcgtaa tagctgacga agaaggagac ggcgttatcg gggaacagat
    28561 cccgcatctc cgaatacagc tgagccgata gcgtcttgtt cggcgcaatg atcagcgtgg
    28621 gccgctgcat cgcctgaatg acgtttgcga tcgtgaacgt cttaccgcta ccggtaatcc
    28681 ccagcagggt ctgccgagtc gccccttcct tgaagccccg caccagcgcc tcgatggctg
    28741 ccggttgatc ccccttcgga ctgaaatcac ttctcaacgc aaagggcatg ctcgtctcgc
    28801 gacctcaaag cgcgtatcgt cgcacgaagg cgtaaaccgg acaaccaagc cccggtgact
    28861 cgaagcccct gcaatacgcc gcccagacgc atcttcggcg gcgccggcgc gacacgggcg
    28921 ggcggcgctc tcttggccag ctttctggca gtaccaatgc gaagagcggg aaacgctttc
    28981 gcgcgtcccg ctctggagca gctcgacagc gagctgtagc gttgagctgt gacttttcag
    29041 cgcttcgagt actggaagcg cttgcgggcg cccgcctgac cgtacttctt acgctctttc
    29101 ttgcggtcgt cgcgcgtgag gtaacccgcg cgcttgagcg tacgacggcg ttccagatct
    29161 tccgaggtca acgcacggga aatcccatgg cgcatcgcct cggcttgcgc gctgtgaccg
    29221 ccaccggcca cgctcgccac cacgtcatac ttatcctgca gttcgagcag ctcgagccct
    29281 tggcgcacca ccatgcgcga agtctctcgc tcgaagtact cgtctacgtt cttgccgttg
    29341 acggtgatgg cgccgcttcc cggctgaatc cagactcgcg cgacggcggt ctttcgcttc
    29401 ccagtggcat acgtcgattg cgctgaaggc atgatctatc tcggatctag cttggaagag
    29461 ccgtggctct tatacgttga gagcctcggg cttttgggct tggtgggggt gctccgcctc
    29521 gccgtagacc ttgagcttct ccagcatctg gcggccgagc acgttcttgg ggagcattcc
    29581 cttcacggcc agcgtgatcg gcgcgtccgg gtgacgcacg acgacgtcac gatacgactc
    29641 gccccggatt gaacctgggt agccgctgtg gcggtagtac atcttgtccg tgaccttcga
    29701 acccgtcagc ttcactttgc tggcgttcac gacgatcacg aagtcacccg tgtcgacgtg
    29761 cggagtgtag accggcttgt gcttgccacg gaggaccgac gcgatcttgg aggccaaccg
    29821 cccaaggggt tggttcgtcg cgtcgaccac gaaccacttc ctggtcaggt ttgcttcggc
    29881 cggtttggca acgtacgtcg acatgttcta aaagagctcc tcgaaggggc gcattcctag
    29941 cgttcagtgt tcttcccgtc aagcaagagt gtgggtgtga ggccgaaaac tggacacgcc
    30001 tggctcatgg tcgaggggaa accgggttta ggccgccgcg ggaggggggt aaagctgtaa
    30061 agtcttcaag gccgttctcg gactccggct gatgtaggcg ccgaccatac ccgcgtaggt
    30121 cgaccgtgta gccagcctat ccactttcgc cagaccgagg cagtgggaac cggatcccca
    30181 acctcaccct cctgtctcga taacctgagt catcgtcact ggttaagtag gcgggccccg
    30241 ggtgtcgagg aggatacccg gacggcgcat ggggccccgg gcagcagccg cggacatgta
    30301 atgcatccgg cgatcacgcc cgcgaacagg tcacatccag cgatataaat gccaagttag
    30361 ccggaaacaa cggccggagg cggtcgatat actagggagc taagttatgc cgcccaccgg
    30421 caacaatcgc ctccttgagc gactcgcgtc cgaccgggtc atcactcccg agcaactcga
    30481 ggctgcggcc aattggatgg cccgcaacgg cgatcgagcg gaggaggccc tgctggagct
    30541 gggtgtgctc gaagaagcag ccctcctcaa gtacttggcc gcgctgcatc gaacgcggtt
    30601 cgtaagtacc gaaaagctgg caaaagccga gatcgaccgg ctgacgctgg acaaagtccc
    30661 gaagaagctc gccgagcggg attgtgtgtt tccggttctt tacgatcctc aggccgcatc
    30721 gctcagcgtg gtgacaccgg atccggacaa cgccatcgcc ttgcaggatc tgcagctcgc
    30781 atccggcgtc aaagacgtac gcgccttcgt gggccggccg cgcgccgtaa aggccgcaat
    30841 caacaaagcc tataacgggg atatccacgc gttcgcgaat ctggatcgcg aagcgcacgc
    30901 gcagttcacc acgatgctca atgtttatga gcgcaacctg gtgagcgagg agagcctggc
    30961 ggtggcggtg gcgcgcgaag ccgtcgggga acgagtgctg accggcgctg acttggagca
    31021 agggcggcgt gccgcctcgg gtggcggccg cgggatcacc gacgactcgt atctcgagac
    31081 gctcaacgtg ctcgtcaact tgctcgaaaa ctcgcgcgca gatctgcggg ggcactcggc
    31141 ccaagtcgcg cggctcatga agaaggtggg cgagcgcatc ggtgtgtccg aaatttctcg
    31201 cgccgccctc caagccgccg gctacttgca tgacatcggc aagatgggca cgtatcacct
    31261 gacggcgctc aacgtggccg aatacgaggg ccaccgcacg tccgcgcaga agcaatatcg
    31321 agcgccgctc cgtctgctcg aagccgtgca tctacccgcc gaggtgacgc aagccatcca
    31381 tcacatgtac gagcggtacg acggccaggg gctgcccgac ggcatctcgg gcaaagaaat
    31441 tccgctcggg gcccgcctgt tggcgattgc cgacacctac gcggatttga cgcaaaaccc
    31501 gcgcaatccg ttccgaaaaa ccttacgtcc cgtgcaggcc tgcgaagtct tggagcgtta
    31561 caagggcggg attttcgatc caaacctagt ggacctgttc aagcacaccg tcacgggtga
    31621 agatctcaag gcgcgtctgc tcgccaatcg tcatcgcgcg ctactcatcg accccgaccc
    31681 cgaagagacc acggtactcg agctgcgcat gatcgagcag ggcttcgaag tgttccaagc
    31741 ccgtaacacc gaagccgcgc tcaaacagct cgagaagggc gaaatcgaga tcgtaattag
    31801 cgaagtggac ctcgcgccag gagatggctt tgcgcttctc tctcaagtgc gcggccaagg
    31861 ctacggccaa gggctgcctt gggttttcct caccagccgt gcgggccgcg cggaggccca
    31921 acgcgcgttc gagctcggcg ccgcggactt catggccaaa cccgtggcca ccgacctatt
    31981 ggttgccaag ctgaagcaaa ttttggagcg cggtgcgcac ggtacccgta ccgcagctcg
    32041 cggcgtctca ggatcgctca gtgagatgag cctgcccgat atggtgcagg tgctgtggca
    32101 cggccgtaag acgggctcgc tcaagattcg ttccgggacg aataccggag agattcattt
    32161 cgtggaaggc aatatttaca acgctttgta cgctgcgctc cgcggcgaag aagcgtttta
    32221 cgccatgctc gcactcaccc agggggagtt cgtgttggac ccgaactttc gggcgcctca
    32281 gcaagttgtc caggccaccc ccgaggcgtt gctcctggaa ggaatgcgac ggttggatga
    32341 aaacgaacgt tgaaagcgca gctcggtcac gcgggtcgga gtcacgccgc gagcgaggtt
    32401 ttatgcccga cctcgtcgga gagcagggtc agcacgtgat gggctgtggg caaacaatcg
    32461 aagagcgcgc tcgtgagatc gctacgcgag cactctcctc cgtgttaagg ttctgactcg
    32521 gcagaggtgg attgtgccgc tcgtgcctcc tccagtactc ggattcaata ccaacatccg
    32581 ctatcgcggc ctgacgtttc acgttcaaag tgaagactca ggggtgcgca cgccgcgcat
    32641 catgacgcac gtgttcgcgg atggcgggcg catcgtcaag agcgcgcgca cggactactc
    32701 cgcgttcttg gggcgagaag atctgcgcga agtcgttcgc aagatgatga aggagcagca
    32761 caaggcgatg ttgatcgcgc tgcgctcggg tgagttcgat gcgcagctag agaaagcttg
    32821 cggcacattg cct
//
LOCUS       DASTKU010000513        18468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_98498,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000513 DASTKU010000000
VERSION     DASTKU010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 18468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18468
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98498"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VFQ61_17720"
     CDS             complement(<1..1069)
                     /locus_tag="VFQ61_17720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET9956354.1"
                     /translation="MTGGNMGSEPTLARRLLTLAWPVALSRLGIMGMGVCDAMVVGQL
                     APRELAHQALGWAPTSVLLVASIGMLTGVQVLAARTVGAGHPERAAGALQRGLVMSLL
                     AGAVAVAFSRFVGAHVYAAFGVSGDLIAPSTRIMQVLMLSVPMHLCYVSGAFFVEAVE
                     RPLLSTYAMWAANLVNLVLNLWLVPRLGALGSAWATVGARFFLAATMLACIFKLPDAP
                     RLGLHRRCAWPGYKQFLSVGAAAAISHAAESSAFSGMTIVAGRLGGSAVAAYQILLNL
                     LAIVFMFALGLATATGVLASQATGRGAPREAARVSWHGLALNTGLLIAIASAIVLFAL
                     PIARAYTADLKVAMLVASIMPI"
     gene            complement(1179..2234)
                     /locus_tag="VFQ61_17725"
     CDS             complement(1179..2234)
                     /locus_tag="VFQ61_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956355.1"
                     /translation="MGIGSFALKSCRVLQVCLPGMVLACCGKSSEPSDSGSAGGVAGA
                     TASAGQGGESGRTDSTGVGPSGGRSSGGAAQGGGTSGAAGAGGNSTGGALSAGGSDAG
                     GAVGGPSGKITITSLYWTPESPLGVSSISARFSNSVLDSECTRQTFGDCYLETCPQTE
                     MQGSEPDAGVLTVTSIGAARGSVQQSIIPDTSRGTYQAWTQTGPVFMPNDVLQIQANG
                     GEVPPFQDTVSFPTELVVASPAPLAASERLSVLEVAPDSDFELSWDGPVADDVILVIQ
                     SDVTVQPTLVCHVPSTKRRFAVPRAALAGVPRDNWGEFYVVREHPLKAGKFDVLIRLA
                     TLLVDTDKSHRVRFHVP"
     gene            2917..3693
                     /locus_tag="VFQ61_17730"
     CDS             2917..3693
                     /locus_tag="VFQ61_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956356.1"
                     /translation="MAANASRVFGEDLPDSDPSMLALAPDARIAVARVWLSRSQNEHK
                     TSFLFEHVLNAATALEADGPVLTLARRAIGDELRHAELCRRVAERYARSENPKAAEMS
                     PIPQPRFRGCSERESALLCLVMHCCVNETIASAYLKHCLDAARGNVARVALRCLLRDE
                     VDHSRLGWAHLASSRVDESERRTVGDAMPELLHAVRAAWLASDDDLSEAAPEGHGSLP
                     IRDVAAAVLAAVNDLIIPGLAHVGVDPSRARSWLSRQPLA"
     gene            complement(3710..4975)
                     /locus_tag="VFQ61_17735"
     CDS             complement(3710..4975)
                     /locus_tag="VFQ61_17735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9956357.1"
                     /translation="MNTPAVFTPYQKLVVALLAFLQFTIVLDFMILSPLGAMLLEQLH
                     ITTQQFGLVVSAYAFSAGASGLLAAGFADRFDRKRLLLFFYVGFLVGTLCCGLAPDYR
                     SLLIARIVTGLFGGVIGSISMAIVADLFGIEVRGRVMGVVQSAFAASQVLGIPLGLWL
                     STRWGWHAPFLLITAVGVAVGFVILFRVRPIDAHLRDGQRRNAFKHLLSTVARRPYLK
                     GFAATIFLATGGFMLMPFGSAFAVNNLGVSLHQLPSVYVATGAFALIGGPIIGRISDS
                     VGKYRTLFVGTVVGALLVLAYVQLGRTPLWIVITLNVILFLAISARMISASALTSEVP
                     APQDRGAYMSVNSSVQQISGGVASALAGLIVVQTQSGKIERYDVLGYVVVLAMLATLG
                     FMYSIHRMIAARRRGPVAGGEIVERPASG"
     gene            5546..6220
                     /locus_tag="VFQ61_17740"
     CDS             5546..6220
                     /locus_tag="VFQ61_17740"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HET9956358.1"
                     /translation="MSAAYSDRPLPALSPQADLDPDNDLEVGPPNAVPDCEPRLEAAG
                     VTYSRAEIPLHRGRQGIATCGANDAVIYRTGPTGAQLRPPALVTCRLALALADLEQLT
                     QELASRHFGSRVRTINQGGTFNCRKMARFRLVSEHSFGNAIDVRSFTLSDGRTISVQR
                     DFGTLGKPPLNNRSKFLRELGEQAFDRAIVSVSLGPYWDALHRDHFHFDMARYRVDGS
                     RPGSTR"
     gene            6500..7621
                     /locus_tag="VFQ61_17745"
     CDS             6500..7621
                     /locus_tag="VFQ61_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9956359.1"
                     /translation="MNTRSHRSSPPVTGRRNWIFGCAAMCLAGGLSGCAKSQSAQEAG
                     AHASSSAGAELKAPTSLSLLNVSYDPTREFYAEVNSVFSKKWKAEKGQELAIKQSHGG
                     SGKQARAVIDGLEADVVTLALAYDVGALASKAQLLPGNWQTRLPNNSAPYTSTIVFLV
                     RSGNPKKIVDWSDLARPGVQVITPNPKTSGGARWNYLAAWGYALQKNNGDASKARSFV
                     GQVLKNVPVLDSGARGATTTFVERGIGDVLVGWENEALLALNELGQGKVEVVVPSTSI
                     LAEPPVAVVDKNADRHGTRAAAEAYLNFLYTAEGQELAAKHHYRPRQPDILAKHTAQF
                     KELKLFTVDEVFGGWDKAQKEHFDDGGTFDQLYQGGSGK"
     gene            7624..8448
                     /gene="cysT"
                     /locus_tag="VFQ61_17750"
     CDS             7624..8448
                     /gene="cysT"
                     /locus_tag="VFQ61_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HET9956360.1"
                     /translation="MLRRQRGVLPGFRLSLSYTLLCLGLLVLLPLSALLYRSTSLGVS
                     AIVDTVTTNRALLAFQLSFGAALVAACINAVFGVLVAWVLVRYDFPARSWVDALIDLP
                     FALPTAVAGIALTSLYSTRGAIGALLAQTGLQVAFTRVGVVLALVFVGLPFIVRSVQP
                     VLEELDPEVEEAAEVLGASRFETFRKVIFPALLPALLTGFALAFARGLGEYGSVVFIS
                     GNMPNKTEIVPLLIVTKLEQYDYVGATALASVMLLISFALLFIVNLLQKWARKAEA"
     gene            8505..9398
                     /gene="cysW"
                     /locus_tag="VFQ61_17755"
     CDS             8505..9398
                     /gene="cysW"
                     /locus_tag="VFQ61_17755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HET9956361.1"
                     /translation="MRGVGWGPPVLLAFALGFLLLCVGAPLLTVFASAFSRGIGPFLT
                     ALADAETLSAIRLTLLTASIAVPLNVAFGMAAAWLIAKYEFWGKNALITLIDLPFSVS
                     PVIAGLIFVLLFGSQGVFASQLRAYDIKILFAVPGIVLATTFVTFPLVAREVLAVMQA
                     QGSDEEEAALTLGASGWQILWHVTLPKIRWGLFYGVVLCNARAMGEFGAVSVVSGHIR
                     GETNTMPLEVEILYNEYNFVGAFAVASLLAGLALITLAAKHGMEFASSLKARRARFEE
                     RRRAVSTEPVTPSLAALSEES"
     gene            9395..10501
                     /locus_tag="VFQ61_17760"
     CDS             9395..10501
                     /locus_tag="VFQ61_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9956362.1"
                     /translation="MNSVASSRPAAAKRDTGAAVATTNVCKRFSGSTGPAAVDNVSFE
                     APKGKLTALLGPSGSGKSTLLRMIAGLEQPDQGRVWIGGQDVTEISPRLRRVGFVFQS
                     YALFRHLTVFDNVAFGLKLRRAPRKQIEERVTALLELVQLPEYATRFPSQLSGGQRQR
                     VALARALATDPEVLLLDEPFGALDARVRVELRRFLLELLAETHTTTLLVTHDQEEAFE
                     LAEHVVLLSEGRVAQAGSPHALYDDPVNPFVASFIGSASRFTGRVSNGRAALGAWTMD
                     APSGAKEGSTVHAFIRPHDVRLERASGPLGQRAEARVERVTRLGAKVQVSLLLSDAIP
                     FTTELAKPQLDELGIGPGDRVHVDVKGAKLFLDA"
     gene            complement(10549..13818)
                     /locus_tag="VFQ61_17765"
     CDS             complement(10549..13818)
                     /locus_tag="VFQ61_17765"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9956363.1"
                     /translation="MSLRDTHVYEFGPFQLNGIERQLSCNGQLVPLTDKVFELLLLLV
                     QNHGRVLAKAELMNKLWPDTVVEENNLTVNVSTLRKALGEGPSERRYIETLARRGYRF
                     VCQVRALPATSAAPVPRRYQPTFVGREQELARLEQFLEAASEGHGRVVLITGGPGMGK
                     TELSEQFLARLHDEPDRYIVATGRCLEQYGAGEVFLPFLEAIRALLMGFDGEQVSEVL
                     RRHAPTWCNQFPSALIYSDGPESSSREALGATSARMLRELADALEALSQIKPVVLSLE
                     DMHWSDPSSSDLLRMLAQRIGRWRGLILATFRDEQVELGNHPLKNIRRELSAHDLCEE
                     IALPLLRPEAIANYLDARCEPHDFPSGLAELIAQRSEGQPLFATRLVQLLIERGDIEQ
                     SEGRFALRKTVPELNLGVPETVRGLIERKLELLSEPDLRALQYASVIGAEFGSLTLAT
                     LLGEDEVALEERLDQLARLNRLVESLGEERLPDGRFSVRYRFAHWLYQSVIYDGLAAK
                     RRMLLHAQIADSLLLEYGGETQRVAGQLASHLKAARDFPRAIHYVMLAADNASRLHAN
                     REATQHYTEALSLIPELSSAERVAYSIVLRYNLGWCHFKVGDLAASVADFEAMLELAC
                     APEFVNEEPDCLAARERVFAYFAEPWRDAFGLCDMARMPNQSSALGPSAIQCEAYWAL
                     CYILLCVDRLDEMGTKVRAFLTLAEVWGNEPRRAEALAWMATRELKLGNLQATNKLLE
                     AATPRARAIGHRRALVVALHAQGRAYDMISAYERAEAMYTEAVGLAIEAEGRMDCLLG
                     LARARIDLGRISAALAALGEANDIARRTLQESSMQLVSAGLGRLFLELGGLDQAERHT
                     LNACELGERSGLPTGVLHALATLARVHVQRGDFAAAGAVLSKAEALLGGELSAGLRDA
                     TGLASAPSAATPESLRGERAARERQRLHFDLARAEYCLARGDFEFATTHARGLLESAR
                     TIGARKHIALAHQLLARVSLARGEPLAARGELAAALEILTDCPMPFLSCRLHAALNTA
                     ELALGRAPAARQAFQRALEIASQVTNEIEDDTVRSIWLGSALVVQLRGERRGASFAAR
                     S"
     gene            13911..14471
                     /locus_tag="VFQ61_17770"
     CDS             13911..14471
                     /locus_tag="VFQ61_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956364.1"
                     /translation="MIKVESHFAFLAGRTHLCAATLFSSIFLGAVSCESPRAHFSRAG
                     GSIERVLPSNSQSVGQLEIVGSLASTEACPGAAKPSSELQSSGVLRFESLNAARPAAL
                     LADTPAPTQLAHYELPPGLYAINWVPKLAVDDDSPHQMLRVSDPAVINIFAGRVTRLE
                     VERSTSDCDVLSMHDPETEIWAAARP"
     gene            14828..16630
                     /locus_tag="VFQ61_17775"
     CDS             14828..16630
                     /locus_tag="VFQ61_17775"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9956365.1"
                     /translation="MALALFAGCAPAPRALAPQSVTAKDTHSSPFAHEESAAVAASPN
                     EMEVPVGGAARAELSKREVNPADPAPPAPAPRREEAKRASRVPVVGAAPKPLASTTPN
                     APSSSAPTAPIPSAASEYNTEAYAAIQENSFQSAKERPLSTFSVDVDTASYSNARRFL
                     AEGSLPPRDSIRVEEWVNYFGYSYAAPTGPQPFAVHTEVTDCPWDAQHRLVRIGIQGR
                     DVPETQIPPRNLVFLVDVSGSMMSSDKLPLVQRALALLTSSLRPQDSIAIAVYAGSSG
                     LALPPTSGRDRSKILRALEQLNAGGSTNGGEGIRLAYNVAREHFEAGGINRVILATDG
                     DFNVGTTSEGELTRLIEEQRKTGIFLTVLGFGSGNLKDATMEMLADKGNGNYAYIDSL
                     AEARKVLVREAGATLVTIAKDVKLQVEFNPARVAQYRLIGYENRALADRDFNDDSKDA
                     GEIGAGHSVTALYEIVPFAAASRPVPGVDPLKYQTTTALSPAARGDELMTVNIRYKEP
                     NADKSLKLTRTVTDSNTPLARTHVDTRFSIAVAEAALLLRRGTLGSGSLKQVRELADQ
                     ARGPDPHGDRREFVAMLDAAMKVQRGVPAAQVAR"
     gene            16829..17878
                     /locus_tag="VFQ61_17780"
     CDS             16829..17878
                     /locus_tag="VFQ61_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956366.1"
                     /translation="MQRLVTALCAILSSGLLSTGTPSGKRAIGPELTSSQIQDTGSSE
                     GVSSRRSSELSGLPQGTLNSPQYASIARAPLTQNAPAHNAPAQRGGNAAAVRIPISRG
                     VMILPQGCAGLDSTFDLLIHFHGAFTTTEPRLMQSGIRSVYVVENLGNFSGPYENAFP
                     SAASFPQHLDALLSRVKTACPTRNLAIGRIALSGWSAGYGAIYRIVAHPSNAQRVDAI
                     LLADGMHVGFEPGSHRVRAAAMQPFLDFAEQAASGRKLMAVTHSSIVPPGYASTTATA
                     TFLADQLGLPSLAPAQPPRDGMEATQYRAKSGLAIMGFAGGDAKAHCDHLYAIQDTLW
                     SRLRARWSRPVNVLQ"
     gene            complement(17902..>18468)
                     /locus_tag="VFQ61_17785"
     CDS             complement(17902..>18468)
                     /locus_tag="VFQ61_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9956367.1"
                     /translation="EVNRGRFREDLYFRLSVISVRLPPLRERLEDIPVLVNAFLEEMD
                     ATSMQHLFTDPVYRELAQHDWPGNVRELRNYVERALVLEDVGPASLSPNSRRTDSTRE
                     APDSSDLPKFHPDEPFKVAKERLISRFERAYLEGLMAWAGGNVSRAARKAKIDRMYLY
                     RLLQRYEMRRDNSPEPPPDSPPAESPQE"
BASE COUNT         3526 a         5964 c         5669 g         3309 t
ORIGIN      
        1 cgataggcat gatgctggcc accagcatcg caaccttcaa atccgcagtg tacgcgcgag
       61 cgataggaag agcgaacaac acgattgcgc tcgcgatggc gatcagcaac cccgtattca
      121 acgccagccc gtgccagctc acgcgagcgg cctcgcgcgg tgccccgcga ccagtggctt
      181 gggaggcaag tacacccgta gccgtggcca gtccgagcgc gaacatgaac actatcgcca
      241 gcaaattgag caggatttga taggccgcaa ccgcagagcc ccccaatcga ccggctacga
      301 tcgtcatccc agaaaaggca ctggactccg ccgcatggct gattgcagcg gcggccccca
      361 ccgacaagaa ctgcttgtac cccggccaag cacagcgccg atgcaacccc aatcgtggcg
      421 cgtccggcag cttgaagatg caagccaaca tggtggccgc aaggaagaag cgggccccca
      481 ccgtcgccca agccgagccg agcgcgccga ggcgcggcac caaccacagg ttgagaacca
      541 gattgaccaa gttcgccgcc cacatcgcat aggtggagag caacggccgc tcgacggcct
      601 cgacgaagaa cgcaccactc acgtaacaaa ggtgcatcgg cacggagagc atcagcacct
      661 gcatgatgcg cgtcgaggga gcaatcagat cgcctgaaac cccgaacgcc gcataaacgt
      721 gagcgccgac aaagcgggag aacgccaccg caaccgcgcc ggccaacagc gacatcacga
      781 gcccccgctg cagtgccccc gccgctcgtt ccggatgccc cgcccctacg gtccgtgccg
      841 ccagcacttg gacgcccgtg agcatcccga tgcttgcgac gagcagcacg gacgttggcg
      901 cccagcccaa agcttgatga gcgagctcgc gcggagcgag ctgccccacc accatcgcat
      961 cgcacactcc catccccatg atcccgagcc gggagagcgc gaccggccac gccagagtga
     1021 gtaaacgccg ggcgagtgtc ggctcagagc ccatattccc gccggtcatc cacgcgcatc
     1081 ttcggtctag tgctccacac cgggcatgca ctaacacagc tggaggtcag ccgctcccgc
     1141 gccgcaaccg cgcggaatcg cgcggcaccg ctgcatactc agggtacgtg gaatcggacc
     1201 cgatggcttt tatcggtatc gaccagcaag gtcgccagcc gaatcaacac gtcgaacttg
     1261 ccggctttga gtgggtgctc acgcacgacg tagaattcgc cccaattgtc ccgaggtact
     1321 ccggcaagcg cggctcgggg cacggcgaaa cgacgcttgg tcgaaggtac gtgacaaacg
     1381 agcgtgggtt gcacggttac atcggactga atgaccagaa tgacatcatc cgctacgggg
     1441 ccatcccaac tgagttcgaa atcagagtca ggagctacct cgagtacgga caaacgctcg
     1501 gaagccgcga gaggtgcggg agatgccacg actagctccg tcgggaacga gacggtgtcc
     1561 tggaaggggg gcacctcgcc gccattcgcc tggatctgga gcacgtcgtt cggcatgaac
     1621 acgggccccg tctgcgtcca agcttggtat gtcccccggc tggtatccgg gataatcgat
     1681 tgttggacgg acccacgcgc ggcaccaatc gaagtcacgg tcagaactcc cgcatctggc
     1741 tctgaccctt gcatctcggt ttgcgggcac gtttccaggt agcagtcgcc gaaggtctgc
     1801 cgggtgcact ccgaatcgag cacgctattg ctgaaccgcg cgctaatgct gcttaccccg
     1861 agggggctct ccggtgtcca gtagagcgag gtgatcgtga tctttccact gggtccccca
     1921 acggctccgc ccgcatcact gccaccagcg ctcaaggcgc ctccggttga gttcccgcct
     1981 gctcccgcag cgcccgacgt gcctccgccc tgagccgcac caccggaaga gcggccgcca
     2041 gaggggccaa ctccggtcga gtccgtccgc cccgattcgc ctccttgtcc ggcggacgcg
     2101 gtagcgcccg ccaccccacc cgcggaacca ctgtcggacg gctccgagct tttcccacaa
     2161 cacgccaaca ccatccccgg caagcagacc tgcagaactc gacacgactt cagcgcaaac
     2221 gacccaatac ccatcttgaa cccacgctta gcacagagcc gagcggttgc cgtcatccaa
     2281 cgacgttgcg agcttcgggt tcgttaagaa gcgatccgct cgccaagatt gactgcggcg
     2341 gctgaagaag acatcgtgaa gcggtgtccg aatccggggt tcgctcgctt tttcacacgc
     2401 tcgtcgtcgt gggcgcttcg ctcactgcat gcggtggaaa gacagatacg gttgcgaccg
     2461 cggcaggtgg ttcgagtagc ggcggccgga cccagactgg acaaggcggg ctcggtacgg
     2521 gtgggctttc cggctcaggc gcggggccga gttcagcact cagtccacga gattgtggag
     2581 cgccggcgca atggaagtgt gtcgactatg attcgctgtc cggctgccac tgcgacccga
     2641 gcgccccgac ctcggcccaa gattgttcaa cgccgtatca cttcacgtgc gaacgaagct
     2701 ggtgggaact cggggctcaa aggtctgaag ggataggctg ccattgcgca agccaggcgt
     2761 tgactccaca ggactgcgtt cataccgagc agttctggtg cgccgtgtat gcgccgtatt
     2821 tcgcggattg cgattgtgag ccggatgctc cgctcggccc cggcgattgc cgcggcggct
     2881 gcgagctctt cagttgcgca aacgaggaac cccgttatgg ctgcgaatgc ctcgcgtgta
     2941 ttcggtgaag acctgcccga cagcgatccc tcgatgctcg cgctcgcgcc cgatgcgagg
     3001 attgcggtgg cgcgcgtctg gttgagtcga tcccaaaacg aacacaagac cagctttctc
     3061 ttcgagcatg ttctgaatgc ggcgaccgcg ctcgaagccg atggcccagt actcacgctc
     3121 gcgcgccgcg caattgggga cgagctgaga cacgcggagt tgtgtcgccg cgtcgccgag
     3181 cgctacgcgc gttccgagaa ccccaaggcc gccgaaatga gccccatccc gcagccgcga
     3241 tttcggggtt gcagcgagcg cgagagtgcc ctcctgtgcc tggtgatgca ttgctgcgtc
     3301 aacgagacga ttgcgtccgc ttatctgaaa cactgtctcg atgccgcgcg gggaaacgtc
     3361 gcacgggtcg cgttgcgctg cttgttgcgc gatgaggtcg accattcccg gctcgggtgg
     3421 gcccatttgg cctcgagccg agtcgatgaa tccgagcgcc gcaccgtggg ggacgcgatg
     3481 ccggagttgc tacatgcagt gcgagcggcg tggttagcga gcgatgacga cttgtccgaa
     3541 gctgcgcccg aggggcacgg ttccctgccc atccgcgacg tagccgctgc cgtgctcgcc
     3601 gcggtgaacg acctgataat tcctgggctg gctcacgtcg gcgtcgaccc gagccgcgcc
     3661 cgaagttggt tgagccgcca acctctcgct tgatcgttcg agcgaacgct catccagatg
     3721 ccggacgctc cacgatctca ccacccgcga cggggccacg acggcgcgct gcaatcattc
     3781 tgtggatgga gtacatgaac ccaagcgtcg cgagcatggc caatacgacg acgtagccga
     3841 gcacgtcata acgctcgatc ttgccggact gggtctgcac cacgatcaac cccgccagcg
     3901 ccgaggctac accgccagaa atctgctgaa cggaagagtt gacggacatg tacgccccgc
     3961 gatcctgcgg agcgggcacc tcggaggtca aagcgcttgc cgagatcatg cgcgccgaaa
     4021 tggccaagaa taggatgaca ttcagcgtaa tcacgatcca gagcggcgtc cggccgagct
     4081 ggacgtacgc cagcacgagc aacgccccga ccaccgttcc gacgaaaagc gttcggtact
     4141 tgcccaccga atcactgatt cgcccaatga tgggcccccc gatcagcgcg aacgctccgg
     4201 tagcgacata gacggagggc agctgatgca ggctaacccc gaggttattg acagcgaacg
     4261 cgctcccgaa cggcatcaac atgaagccgc ccgtcgccaa aaatatcgtg gccgcgaagc
     4321 ccttcaagta cggacgccgt gcgaccgtcg agagcaggtg tttgaaggca ttgcggcgtt
     4381 gtccgtctcg taagtgggca tctatcggcc taacgcgaaa taagatcacg aaaccaacgg
     4441 ccacgccgac cgcggtgatg agcaagaacg gagcatgcca accccaccga gtggacagcc
     4501 ataacccgag aggaatcccg agcacctggc tcgccgcaaa cgccgactgc accaccccca
     4561 tgacccgacc gcgcacctcg ataccgaaca gatccgcgac gattgccatc gagatagagc
     4621 caatcacacc gccgaacagg cccgtcacga tccgagcaat cagcagactc cggtagtctg
     4681 gggccaagcc gcagcacagc gtgccgacca gaaagcctac gtagaagaac agcaggagcc
     4741 gtttgcgatc gaaacggtcg gcgaaaccag ccgcgagcaa acccgacgct cccgcactga
     4801 atgcatacgc ggagaccacc agcccaaact gctgcgtcgt gatgtgcagc tgttcgagca
     4861 gcatagcgcc gagtggcgac aggatcatga agtcgagcac gatcgtgaac tgcaagaagg
     4921 cgagcagcgc gaccacgagc ttttggtacg gtgtaaagac agcaggtgtg ttcatgtggg
     4981 gggcgcttcg ggccgcagtg cctcgatcgc gagcgacgac gtcagttcca atgccacatc
     5041 actcgatttg aaaaggtcca ttgccagaat tcccttggcg tgcacgtgca ttttcgtgag
     5101 ctggcgcaag ggaacgaacg ccacttgggc tgctccaggc taaccctggc tcaattcttc
     5161 cattgaatcc gcaagccctc cccgaaccca cttccggttc gaactctgtc cgagtggcct
     5221 cgaccaagcg cgcatcgtct cttgatagag cgcgaagatg aggccgttac tgcgagggct
     5281 ggatgtttgg agaagctggc acgtgttcag aaagccagta acttgttccg gtatcggaat
     5341 gcgggtccgc gaagcaggtg ttgtttggtg gtgttggctt ggattggcaa gtgcttgcgc
     5401 gggagacggg accgctttgc cgcggagccg tcccccgcat gccacgggcc ggacaccgga
     5461 gacttcccga atgccagcgg ccacagtccc accaaacgtc gaagtgcggt cgcccgctgg
     5521 cgctctcgag gaatcgccag cggtagtgag cgctgcctac tctgaccgtc cgctgcccgc
     5581 attgtcgcca caggcagatc tggatcccga caatgatctc gaagtagggc ctcccaacgc
     5641 agtacctgac tgcgagccac ggctcgaagc cgccggagtc acgtactctc gagccgagat
     5701 cccgctccac cggggccgcc aaggcatcgc gacgtgcggc gcgaacgacg ccgtgattta
     5761 ccgaactgga cccaccggag cccaacttcg ccccccagcg ctggtcactt gtcggctggc
     5821 gctcgcgctc gcggacctgg aacaactcac ccaggagttg gcgagccggc acttcggttc
     5881 gcgcgtgcgc acgattaatc aagggggaac cttcaattgc cggaagatgg cccgctttcg
     5941 actcgtcagt gagcacagct tcggcaacgc catcgacgtg cgctctttca cgctgtcgga
     6001 cggcagaacc atctcggtac agcgagattt cggaaccttg gggaaaccgc cgctcaacaa
     6061 tcgctcgaag ttcttacgtg agctcggcga gcaggctttc gatcgcgcaa tcgtcagtgt
     6121 gtcgctggga ccgtactggg acgcgctaca ccgcgaccac tttcacttcg acatggctcg
     6181 ctaccgcgta gacgggagcc gtcccggttc aacgcgctga gctgagccgc agcttcgcgc
     6241 gtgcattctc gtcttcgacc tcttgatcgc tgagtcccag ccgactcagt cagcgggtct
     6301 tcgccgtctc gaacagacgc cgcctgaccc tgagcaagcc cgaaaacggg ccgctggcct
     6361 attgacactc gcatgcccgg ctgctatcca cacaaagtcg atcgaatcag tagggtttaa
     6421 agtggaaccc gaacggtcgc tcgaaattca tcgaagcttt tgaggacttt cgcagctgga
     6481 attcttgacc gagttactca tgaatacgcg ctctcaccgc agttcccctc ccgttaccgg
     6541 tcgtcgcaat tggatttttg gatgcgcggc catgtgcctg gccggagggc tctcgggctg
     6601 cgcgaagtcg caatcggctc aagaagcggg cgcacatgca agcagcagcg cgggggcgga
     6661 actcaaagcg ccgaccagcc tctcattgct caacgtttcc tacgatccga cgcgcgagtt
     6721 ttacgcggaa gtgaactcgg tgttttcgaa gaagtggaaa gccgagaagg gccaggagct
     6781 agcgatcaag cagtcgcacg gcggctcggg aaaacaggcc cgcgccgtaa tcgatggcct
     6841 ggaagcggat gtcgtcacgc tcgcgctcgc ctacgatgtc ggggcgctcg cgagtaaggc
     6901 ccagctcttg ccgggaaact ggcagacgcg cttaccgaac aacagcgccc cctacacctc
     6961 tacgatcgtg ttcctagtcc gcagtgggaa cccgaagaag atcgtcgact ggagtgatct
     7021 tgcccgaccc ggtgtgcagg tcattactcc gaatcccaaa acctcgggcg gagcccgctg
     7081 gaactacttg gcagcttggg gctatgcgct ccaaaagaac aacggcgacg caagcaaggc
     7141 gcgcagcttc gttggccaag tactgaagaa cgtcccggtg ctggactcag gtgctcgcgg
     7201 cgccaccacc actttcgtgg agcgcggcat tggagacgtg ctggttggct gggaaaatga
     7261 ggctttgctg gcgctcaacg agctcggcca aggcaaagta gaggtggtcg tcccgtccac
     7321 cagcatcttg gcggagccgc ccgtcgccgt ggtggacaag aacgccgacc gtcatggtac
     7381 acgcgccgcc gccgaggcct acttgaactt cttgtacaca gcggagggtc aggagctcgc
     7441 cgcgaagcac cactaccgcc cgaggcagcc agatattctg gccaagcaca cggctcaatt
     7501 caaagagctg aagctgttta cggtggatga ggtgttcgga ggctgggaca aggcccaaaa
     7561 ggagcacttc gacgacggtg ggacgttcga tcagctctac caaggcggtt cggggaagta
     7621 gccatgctcc ggaggcaacg cggagtacta ccggggttcc ggctaagcct ctcgtacacg
     7681 ttgttgtgcc tggggctgct ggtgctgttg cccctatcgg cgctgctcta ccgatcgact
     7741 tcgctcggcg tctcggcgat cgtcgatacg gttacgacca accgcgcgtt gttggcgttc
     7801 cagctgagct tcggagcggc cctcgtggcg gcttgtatca acgccgtttt cggcgtgctc
     7861 gtggcttggg tgctggtgcg ctacgacttc cccgcccgaa gctgggtgga tgctctgatt
     7921 gatctgccct ttgcgttgcc cactgcggtg gccggcatcg ccttgacgag cctctactcg
     7981 acgcgggggg cgatcggcgc gctgctcgcc cagacggggc tccaggtggc atttacgcgg
     8041 gtaggggtcg tcctcgcgct ggtattcgta ggccttccgt tcatcgtacg cagcgtgcag
     8101 cccgtgttgg aggagctgga tcccgaggtc gaggaggccg cggaggtgct cggggcctcg
     8161 cgcttcgaga ctttccgtaa ggtgatcttt cccgcgctgc tcccggcact gctcacgggc
     8221 ttcgcgttgg cgttcgcccg cggcttgggc gagtacggca gcgtcgtgtt catctctggc
     8281 aacatgccca acaagacgga gattgttcct ctcctcatcg tcacgaagct cgagcaatac
     8341 gattacgtcg gggccaccgc gctcgcatcc gtgatgctgc tcatttcgtt tgcgttgttg
     8401 ttcatcgtca acctgcttca gaagtgggct cgaaaggccg aggcatgagc gctgcattgt
     8461 cgtcgtcttc ccccagtgcg gttcgcggta ggcccaggac tccagtgcgg ggcgtaggtt
     8521 ggggaccgcc cgtgctcttg gcgttcgcgc tcggcttcct actcctgtgc gtgggcgcgc
     8581 cgttgctcac cgtcttcgcg tccgcgtttt ctcggggcat cgggccgttc ctcaccgccc
     8641 tcgcggacgc ggagacgtta tccgcgattc gcttgacgct actcacggct agcatcgccg
     8701 tcccgctcaa cgtggcgttt ggcatggcgg cggcctggct gatcgcaaag tacgaattct
     8761 gggggaagaa cgcgctcatc acgctgatcg acttaccttt cagtgtgtcg ccggtgatcg
     8821 cagggctgat cttcgtgctg ttattcggtt cacaaggcgt gtttgcgagc caactgcgcg
     8881 cctacgacat aaagatcttg tttgcggttc ccggcatcgt gctcgcgact acgttcgtca
     8941 cgttcccgct ggtagcgcgt gaggtgctgg ccgtaatgca agcgcaaggc agcgacgaag
     9001 aggaggccgc gctaacgctc ggagcgagtg gttggcaaat cctgtggcac gtgacgttgc
     9061 ccaagattcg ttggggcctg ttctatggcg tggtgctctg caatgctcgg gcgatgggcg
     9121 agtttggcgc tgtgtccgtg gtttccggtc acattcgcgg ggagaccaac acgatgccgc
     9181 tcgaagtcga gattctgtac aacgaataca acttcgttgg cgctttcgcg gtcgcttcgt
     9241 tgctcgcggg cctcgccttg atcacgctcg cggccaagca tggcatggag ttcgcctcga
     9301 gtttgaaggc tcgccgtgct cgattcgaag agcggcgccg cgcagtttcg accgaaccgg
     9361 taacacccag cctagctgcc ctctcggagg agtcatgaat tctgtagcct cgtctcgccc
     9421 cgccgccgcg aaacgcgata ccggtgcggc ggtcgcaacc acgaacgtct gcaaacgctt
     9481 ttcgggatct acgggtcccg ccgcggtgga caacgtctcg ttcgaagcgc cgaaaggcaa
     9541 actcacggcc ttgctcggac cgtcgggttc agggaaatcc acgctgctgc gcatgatcgc
     9601 ggggctagaa cagccggacc aaggccgcgt ctggatcggc ggacaggacg tgacggagat
     9661 ctccccacgc ctgcgtcggg tcggattcgt gttccaaagt tacgccttgt ttcgacacct
     9721 aacggtgttc gacaatgtcg cgttcgggct caagctgcgg cgtgcgccgc gcaagcagat
     9781 cgaagagcgc gtcacggcgc tgctggagct ggtgcaactg cccgagtatg cgacgcgctt
     9841 cccctcacaa ctttccggcg gtcagcgcca gcgcgtggca ttggcccgag cgctcgccac
     9901 ggatccggag gtgctgctct tggatgagcc gttcggggcg ctcgatgcgc gggtgcgtgt
     9961 agagttgcgg cgctttttgc tcgagttgct agcggagacg cacaccacta cgttgctggt
    10021 gacccacgac caggaggaag ccttcgagct cgcggagcat gtggtgctgc tatcggaagg
    10081 tcgtgtcgcc caagcgggct cgccgcatgc actctacgac gaccccgtaa atccgttcgt
    10141 ggcttcgttc atcggttcgg cgagccgctt taccgggcgc gtcagcaacg gacgcgcggc
    10201 cttgggcgcg tggactatgg atgcaccgtc cggggccaaa gagggctcga cggttcacgc
    10261 attcattcgc ccgcacgacg tgcggctgga acgagcctcg gggccgctgg gtcagcgtgc
    10321 cgaagcccgc gtcgaacgag tcacccggct cggcgccaag gtgcaggtat cgctgctcct
    10381 gagcgacgcg atcccgttca cgacggagct cgccaagccg cagctagacg agctcgggat
    10441 tggcccgggc gaccgcgtcc acgtagacgt caaaggtgcc aagctgttct tggatgcgtg
    10501 agcgagacgg tcggcatgcg gcatttcgac ggagcggaac tcgcattctc agctcctagc
    10561 tgcaaatgaa gcccctcggc gctcgccacg caactggacg accagcgccg aaccgagcca
    10621 gatcgatcgc accgtatcgt cctcgatttc gttcgtcact tgtgaagcga tctcgagcgc
    10681 ccgttgaaag gcttgtcggg ctgccggcgc gcggcctagc gccagttccg ccgtattcag
    10741 tgccgcgtgc aggcgacacg agagaaacgg catcgggcaa tcggtcaaga tctcgagcgc
    10801 ggccgccaat tcccctcggg cagccagcgg ttctccgcga gccaggctca cgcgagccaa
    10861 cagctggtga gcgagcgcga tgtgcttccg ggccccgatc gtgcgggcag attcgagcaa
    10921 accccgagca tgcgtggtcg caaactcgaa atcgccgcgg gccaggcagt attccgcgcg
    10981 cgccaaatcg aagtgtaagc gctgtcgctc gcgcgcagcg cgttcgccgc gcaacgactc
    11041 tggggttgcc gccgacggag cactggcgag accggtcgca tcccgtaggc ccgcactgag
    11101 ctctccaccg agcaacgctt ctgccttgct cagcaccgca cccgcggccg caaaatctcc
    11161 acgttgaacg tgaacgcggg ccaaagtcgc gagcgcgtga agcaccccgg tgggaagccc
    11221 cgagcgttcc ccgagttcgc aagcattcaa agtgtggcgc tcggcctgat ccaacccccc
    11281 gagctcgagg aataaccgac ccaagccagc cgagaccagt tgcatggagg attcctgaag
    11341 ggtccggcgt gcgatgtcat tggcctcacc gagcgccgcg agcgccgcgg agatgcggcc
    11401 caggtcgatg cgcgcacgag ccaagcccag taggcaatcc atgcgcccct cggcctcgat
    11461 cgcgagcccg acggcctcgg tatacatggc ctcggcgcgt tcgtaggccg agatcatgtc
    11521 gtaagctcgg ccttgcgcgt gcaaggcgac tacgagcgct cgtcggtgcc cgatggcgcg
    11581 ggctcgtggt gtggctgctt ccaatagctt gttggtcgcc tgcaaattgc cgagcttgag
    11641 ctctcgcgtc gccatccagg ccagcgcttc tgcgcgtcgg ggctcgttgc cccacacctc
    11701 ggccagcgtc aggaacgccc ggaccttagt gcccatttcg tccagccgat cgacgcacag
    11761 caggatgtaa cagagtgccc aataggcttc gcactggatc gcggaaggac cgagcgcgct
    11821 cgactggttg ggcatacgcg ccatgtcgca caagccaaag gcatcccgcc aaggctcggc
    11881 gaagtacgcg aacactcgct ctcgtgcggc gagacaatcc ggctcctcgt tcacgaactc
    11941 cggagcgcac gccagctcca gcatggcctc gaaatcagcc acactcgcgg cgagatcccc
    12001 aaccttgaag tgacaccagc ccaggttgta acgcaagacg atcgaataag cgacgcgctc
    12061 cgccgatgac agctccggga tcagggacag tgcttccgtg tagtgttgcg tcgcctcacg
    12121 atttgcatgc aagcggctcg cgttgtccgc cgccaacatc acgtagtgaa tggcgcgcgg
    12181 aaagtcgcgc gcggccttca aatgcgacgc cagctgcccg gctacgcgct gggtttcgcc
    12241 gccgtattcg agcagcagac tatccgcgat ttgcgcgtga agcagcatgc gccgtttggc
    12301 ggccaggcca tcgtatatca cgctctgata aagccagtgc gcgaagcgat agcgcacgct
    12361 gaagcgcccg tccgggagcc gctcctcccc gagtgactcg accaggcgat tcaagcgggc
    12421 gagctggtcc aatcgctcct cgagcgcaac ttcgtcctcg ccgagcaagg tcgccagcgt
    12481 gagcgaaccg aactcggccc cgatcacgct cgcgtactgc agcgcacgca agtcgggctc
    12541 actcagcagc tccagcttgc gctcgattag gccccgcacc gtctccggca cccccaggtt
    12601 gagctcgggc acggtcttac gcagcgcgaa gcgcccctcg ctctgctcga tatcgccgcg
    12661 ctcgatcaag agctggacga gccgcgtcgc gaagagcggc tgaccctcgc tgcgttgcgc
    12721 gatgagctcc gccaagccac tcgggaaatc gtgcggctcg cagcgcgcgt cgaggtagtt
    12781 cgcgatcgcc tccggtcgca gtagcgggag cgcgatctcc tcgcacaagt cgtgcgcgga
    12841 gagctcgcgg cggatattct tcaggggatg attgccgagt tcgacctgtt cgtctcggaa
    12901 ggtggccagg attagaccac gccaacggcc aatccgctgg gcgagcatgc gcagcaaatc
    12961 gctgctcgaa ggatcggacc agtgcatatc ctcgagcgac aacaccaccg gcttgatttg
    13021 actgagcgcc tccagcgcat ccgcgagctc acgtagcatt cgcgccgagg tggcgccgag
    13081 cgcctcacgg ctagaagact ccgggccatc gctgtagatg agagcggacg ggaactggtt
    13141 gcaccaagtg ggcgcatgcc gccgcagcac ctccgatacc tgctcgccat cgaagcccat
    13201 gagcagcgct cgaattgcct ccaggaacgg caagaagacc tcaccggcac cgtactgctc
    13261 gagacaccgg cccgtcgcaa cgatgtaccg atcgggctcg tcgtgcagac gcgccaagaa
    13321 ctgctcggac aactcggtct tgcccatccc gggcccaccc gtgatcaaga cgacccgtcc
    13381 gtgcccctcg ctcgccgctt cgaggaactg ttcgagcctc gccagctcct gctcacgccc
    13441 cacgaacgtc ggctgataac gacgcgggac cggcgccgca gaagtcgcgg gcaacgcgcg
    13501 cacttggcag acgaagcgat atccacgtcg cgccaacgtc tcgatgtagc gccgttccga
    13561 aggcccttca ccgagcgctt tgcggagcgt gctgacgttg accgtcagat tgttttcttc
    13621 gacgacggta tccggccaga gcttgttcat gagctcggct ttggcgagaa cgcgcccgtg
    13681 attctgcacc aacagcaaca gcaactcgaa aaccttgtcc gtcaaaggca cgagctgacc
    13741 gttgcacgat agctggcgtt cgatgccgtt cagctgaaac gggccgaact catatacatg
    13801 cgtgtctcgc agggacattg gccctcgggg cggtgtttag caacttttta ggacaagtct
    13861 aatggtgctc ttaaggactc actgacagtg ccgaggcaag ctgcagcaag atgatcaagg
    13921 tcgaaagcca ctttgcgttt cttgctggtc gcactcactt gtgcgccgcc acactgtttt
    13981 cttctatttt tctgggagcg gtttcatgtg agtcaccgcg cgctcatttc tcacgcgcag
    14041 gcggctcgat cgagcgcgtt ttgccgagca attcgcaaag cgtaggccaa ctggaaatcg
    14101 tcggttcgct agcaagcaca gaggcttgcc ccggtgctgc gaagccatcg tccgaactgc
    14161 aatcatcggg cgttttgcgg ttcgagagcc tgaacgccgc gcggccggcc gcgttgctcg
    14221 ccgatacgcc agcgccgacc cagttggctc actacgaact accgccaggt ctttacgcca
    14281 tcaactgggt gccaaagttg gccgttgatg acgactcgcc tcatcaaatg ctgcgggtga
    14341 gcgacccggc ggtaatcaac attttcgcgg ggcgggttac gcggctcgaa gtcgagcggt
    14401 cgaccagcga ctgcgatgtc ctttctatgc acgacccaga gaccgagatc tgggctgcag
    14461 cgcgacccta gcacgtggtg cttggatggt ggggacaagg gctgacctag ccagccgacg
    14521 caggcgtctc ggaacgatcg cctaggacaa tcgagcgcgg cgatgttcag gcgaatatcg
    14581 cgtcttgggg acacgccggc tgaacggtat tcgtctgtat tgaacgcact gccgaaactc
    14641 gccatgtggc gagccttgct ccggcaaagc cagctaaagt ggaagcggcg aagcgaacgg
    14701 gcgtaaggga ggcgagcatg aggcgttctc agcggcggag gttctccgca tgccccgccg
    14761 cttcagacca ttcattccgt ctcaatcaaa gcccctccat ccgttcgggc tcgcttctca
    14821 tttaagcttg gctttggcac tgttcgcggg ctgcgcgccc gcgcctcgtg ccctcgctcc
    14881 acaatcggta accgccaagg atacccattc ttctcccttc gctcacgaag agagcgcagc
    14941 cgttgccgcg tcgccgaacg aaatggaggt acccgtcgga ggcgcagcac gcgctgagct
    15001 gtccaagcgc gaagtaaacc cggccgaccc cgcgccgccc gcacccgcgc ctcggcgcga
    15061 agaggcgaag cgggcttcgc gcgtgcccgt ggtcggcgcc gcgccaaaac cgctcgcttc
    15121 tacgacgccg aacgcgccat cgtcttctgc tccgaccgcg cccattcctt cggccgcgag
    15181 cgagtacaac acggaggcgt acgcggcgat tcaggagaac tcgttccaga gcgcgaagga
    15241 acgacctctg tcgacgttct cggtcgacgt cgatacggca tcctatagca acgcgcgccg
    15301 tttccttgcg gaaggcagct tgccgcctcg tgactccatt cgagtggagg agtgggtcaa
    15361 ctactttggt tattcgtacg cagctccaac gggtccgcag ccgtttgccg tacacaccga
    15421 agtcaccgac tgtccatggg acgctcagca tcgcttggtt cggatcggga ttcagggccg
    15481 cgatgtgccc gaaactcaga ttccaccgcg caatctggtg tttcttgtcg atgtgtccgg
    15541 ttcgatgatg tcgagcgaca agctgccgtt ggtacaacgc gcactagcct tgttgacctc
    15601 ctcgctgcgg ccacaggatt ccatcgcaat tgccgtgtac gcgggcagct ccgggctcgc
    15661 gttgcctccc acttctggtc gtgatcgcag caagatccta cgcgcgctcg agcaattgaa
    15721 cgcgggtggt tcgacgaatg gcggcgaggg cattcgactg gcctacaacg tggcgcgaga
    15781 acacttcgaa gcgggcggca tcaatcgcgt gatcctcgcg acggacggcg acttcaacgt
    15841 cggtaccacc agcgagggag agctgacacg actgatcgag gaacagcgca agacggggat
    15901 atttctaacg gtgctcggct tcggcagcgg caacctgaaa gacgcgacga tggagatgtt
    15961 ggcggacaaa ggcaacggca actacgccta tatcgattca ttggcggagg cgcgcaaggt
    16021 gctggtgcgc gaggcgggag cgacgctggt gacgatcgcc aaagacgtca aacttcaggt
    16081 cgagttcaac cccgcccgcg tcgcccaata ccgattgata gggtatgaaa atcgagcact
    16141 ggcggaccgc gatttcaacg atgacagcaa ggatgccggc gagatcgggg cgggccacag
    16201 cgtaaccgct ttgtatgaaa tcgttccgtt cgcagcagcc tctcgccccg ttccaggcgt
    16261 ggatcccctc aagtaccaga cgacgacggc cctctcgccc gcggcccgag gcgacgagct
    16321 gatgaccgtg aacatccgtt acaaggagcc gaacgccgat aagagcctga agctgacccg
    16381 caccgtgact gactccaaca cccctctggc ccgcacccac gtcgacacgc gcttctctat
    16441 cgcggtcgcg gaagctgcac tactgctgcg caggggcaca ctcggctcgg gcagtctgaa
    16501 gcaagttcgc gagctggcgg accaggcgcg gggcccggat ccccacggcg atcgtcgcga
    16561 gttcgtggcg atgctggacg ccgcgatgaa ggttcagcgt ggggtgcccg ccgcgcaggt
    16621 agcccgctag tcgtctcccg tcacattcgg tgcacatctg ccgctcacgt gcgacgtata
    16681 tgctgcgatt ctgcaaactc gcagcacgag gggatgagtc cgcaaccccg ctgtcccgag
    16741 tctacagggc agaaccatcc gccaaggcac gcttggggcg ttacgcggca attcgcgatt
    16801 ggcgcagatc tttctttagc tccggacgat gcaacgcctc gtcactgctc tgtgcgcgat
    16861 cctcagttcg ggacttctca gtacgggaac tccgagcgga aagcgcgcaa tcggaccgga
    16921 gctcaccagc agccaaatcc aggatacggg tagttccgag ggcgtgagtt cacggcgttc
    16981 gtccgagctc tctggcctcc cgcaaggcac gttgaacagc ccgcaatacg cgtcgatcgc
    17041 acgcgccccg ctcacacaaa acgcgcccgc acacaacgcg cccgcacaac ggggcggcaa
    17101 cgcggccgcg gtgcgcatcc caatctcgcg cggcgtgatg atattaccgc agggttgtgc
    17161 gggcctggac tcgaccttcg atctcctgat ccactttcac ggcgccttca ccaccaccga
    17221 gccacgcttg atgcagagcg gaattcggag cgtttacgtc gtcgagaact tgggtaactt
    17281 ttcgggcccg tacgaaaacg cgtttccgtc cgccgcgtcc ttccctcaac acttggacgc
    17341 gctcctctcg cgggtgaaga cggcctgtcc gactcgcaac ctggctattg gcagaatcgc
    17401 cctatcgggg tggagtgccg gttacggcgc catctaccga atcgtggcgc acccgtcgaa
    17461 cgcgcagcgc gtggatgcga tcttgctcgc ggacggaatg cacgtcggct tcgagccggg
    17521 ctcgcatcgc gtccgagccg ccgcgatgca gccgttcctg gacttcgccg agcaggccgc
    17581 gtcgggtcgg aagctgatgg ccgtcaccca ctcttcaatc gtccctccgg gttacgcgag
    17641 caccactgcc actgccacct tcttggccga tcagcttggc ttgcccagcc tcgcaccggc
    17701 gcagcctccg cgcgacggca tggaagccac gcagtaccgc gccaagtcag ggctcgccat
    17761 catgggtttc gccggcggcg acgccaaagc tcactgcgat cacctttacg ccatccaaga
    17821 tacgctctgg tcacgactgc gcgcccgctg gtcgcgaccg gtaaacgtac tccaatagct
    17881 atacgcggag gttcgccgca ctcactcctg cggcgattcc gcaggcgggc tatcgggcgg
    17941 aggctcggga ctgttatcgc gacgcatctc gtagcgttgc agaaggcgat agagatacat
    18001 ccgatcaatc ttggccttac gggcggcgcg gctcacgttg cctcccgccc atgccatcaa
    18061 cccttccaaa taggcgcgct cgaagcgcga gatgaggcgc tctttggcga ctttgaacgg
    18121 ctcatcgggg tggaatttcg gcagatccga ggagtccggc gcttctcggg tgctgtccgt
    18181 gcgtcgcgag tttggagaga gagacgccgg tcccacgtcc tccagaacga gcgcgcgctc
    18241 cacgtagttt ctcagctccc gcacgttgcc cggccaatcg tgctgggcga gctcccggta
    18301 aaccgggtcc gtgaacaggt gctgcatact cgtagcatcc atctcttcga ggaaggcgtt
    18361 gaccagaacc ggaatatcct ctagccgctc gcgcaatggc ggcaagcgaa cgctaatcac
    18421 ggagagccgg aaatataggt cttcgcgaaa acggccacga ttgacctc
//
LOCUS       DASTKU010000514        11049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_98555,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000514 DASTKU010000000
VERSION     DASTKU010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11049
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98555"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VFQ61_17790"
     CDS             complement(<1..840)
                     /locus_tag="VFQ61_17790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9956368.1"
                     /translation="MRIAILTTSYPQWDGDAAGHFVAADALELVRQGHSVSVFCPRDP
                     QFGWCHPDPQTANPAVFSADAHGACGFPGVLPRIRARPWRLLGLLSWLWSARRWLKTH
                     GPFDRLICQWLFPSAWPLARYTRNSVAKLECVVHGSDARLLARLPRPVQRSIVRGLLQ
                     RGAHFRCVSEELAQIVWQAVDFRAATAARALRAELEARIYVHPPALEVEVPLSRSAAR
                     KQLNVITPAPLVVIASRLIASKRVDVALNALRLAGITDIVVLGDGPEQRTLARRYPEV
                     RFMG"
     gene            complement(890..1240)
                     /locus_tag="VFQ61_17795"
     CDS             complement(890..1240)
                     /locus_tag="VFQ61_17795"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HET9956369.1"
                     /translation="MQQARFKPTNVKAPHGGKELEVTWADGHKSRLTNELLRGYCPCA
                     GCQGHSSGLKFQTGGNTELREIEQVGNYALSFTWGDGHSSGIYSFRYLRSLGDLVDEQ
                     GTAQVIAMGELQRV"
     gene            complement(1287..1961)
                     /locus_tag="VFQ61_17800"
     CDS             complement(1287..1961)
                     /locus_tag="VFQ61_17800"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET9956370.1"
                     /translation="MKSDPPRAGLLGQITLDLPAWLQSQLAELPVALTSLDARVQLVL
                     ELARQNIRNSSGGPFAAAVFREADGALVSVGVNRVVPRACSSAHAEVMALSLAQAALG
                     RFDLGAPELGAHQLVASWRPCAMCFGAIPWAGVRSVAIAGSGAEMEQLTGFDEGPIHP
                     QWREELEKRGIRVFDNLASREALEVFREFASSGQLVYNGRGGLPPAADSGREQAAPLT
                     DLGERE"
     gene            complement(1958..2503)
                     /locus_tag="VFQ61_17805"
     CDS             complement(1958..2503)
                     /locus_tag="VFQ61_17805"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9956371.1"
                     /translation="MATVSARDVIRELNLSPHPEGGYYREVFRSPHRVQSHSHDGPRS
                     ASTQIYYLLESGSFSAFHRVSSDETWHHYLGDAVELCVLREAGVERLRLGSALALGER
                     PQHVIPAGEWQAARVTASAHGFALCGCSVAPGFEFSDFEMPTRSDLLRLFPEQRFPEH
                     QELILALTRPSGPPTTSEGTS"
     gene            3013..4272
                     /locus_tag="VFQ61_17810"
     CDS             3013..4272
                     /locus_tag="VFQ61_17810"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9956372.1"
                     /translation="MARPPHEANKPSESATSEPPEGHEQKPFASLLPTRRQFVVGVSG
                     AVIGVACAESHEGGGVPTGTGGTSGGVSAGGAKATGGAPSTGAASSTGGAVSTAGASG
                     GLTSTADARGGSPSTGGAASSGGAASGGVPAGGAASGGVPAGGAASGGVPAGGAASGG
                     TSGGVTRKLASDQVVLGATGIKVSRLAMGCGSHGSNKGSDQARLGVDAFAGLLKYAYS
                     QGINFFETADAYGSHPHVAEAIRQVGRNNVVVLTKTMSQRAADVEADLARFRTELGID
                     VIDIVLLHLKTSSRWATDCEGAMEVLSKAKESGAIRAHGVSCHSLQALRLAAATPWVD
                     VDLARINPAGVVMDSDPTTVISVLREMKTANKGVIGMKILGEGQLADRLDQAIQHAVG
                     LDCIDGFTIGFTSQAQLDQVIQKIAAV"
     gene            complement(4304..4804)
                     /locus_tag="VFQ61_17815"
     CDS             complement(4304..4804)
                     /locus_tag="VFQ61_17815"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HET9956373.1"
                     /translation="MPVAHTAALFTGMRLTEAYLWPSPFAESPAKWGKHYADAFTQPP
                     VFHPDRRAFEWDGDRYTINVFGHGLLGSELFYRARRCNSGFWGALLFATSASAVWEYG
                     FEANGVRPSALDLVYTPIAGLALGEARYWGQHAARSIHSPGVRWIVTALLDPFGEFER
                     GLGAPC"
     gene            5180..5425
                     /gene="rpsU"
                     /locus_tag="VFQ61_17820"
     CDS             5180..5425
                     /gene="rpsU"
                     /locus_tag="VFQ61_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET9956374.1"
                     /translation="MANPSEAVTCKPLEVQVGDKGIERAIKHLKRRMAAEGILRELKR
                     RRHYMKPSVKRRKKMSEAARRRRKRLRADEQQRTGML"
     gene            5586..7241
                     /locus_tag="VFQ61_17825"
     CDS             5586..7241
                     /locus_tag="VFQ61_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956375.1"
                     /translation="MSSPKYPVARSSYRGHAPRTLSGPQQSQLSDPRQPRRQTDARNA
                     AWQSESVRIATAGLRDTAAPREANVRGPGAATLRSLRTQAGKSSQPGRLAKGSSRGAN
                     GAGAGGDHAADDGGVGGTSAPLIRLRYVLGALGAIGMIVWGIPRARAAWDVHSVGSTL
                     ADYALCMAGPTGPELIRDDDAEFRRLIRRRLLAAGPEDAPFARCGKLAVELSSKAELA
                     QVHANKAQAFEEYGLNGGRVPLTALGVDAGVLQRRAEQAWPFVRGSSARLVRPSLGAV
                     EAVHPVPPPRPSLGRGLPATRTLPRNTWQTREALFLSVGNGVGQSLYFSSDAGATFRA
                     SRSAPQAEAYAEGCVGKDPSHPFMLSIGEDGSLRVGSNSADQGSFSSTAVQGEHELLA
                     LACDDSALVVAARREGAQAAQFVLCPQLRSCSPLPLPKTAPFTSLAGSDFDVARSAGV
                     TVLITANRGVVRVVSSRDDGQTWTPQSVAFDAFEHPALRADVTVPTRLLSAGGRLFLY
                     GATSKPNQSYPVLVSDDQGASFRGIGASPEEPGRARVATAPRR"
     gene            complement(7342..8781)
                     /locus_tag="VFQ61_17830"
     CDS             complement(7342..8781)
                     /locus_tag="VFQ61_17830"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HET9956376.1"
                     /translation="MVAQPNYMAQVPEDLWHVAIGPGDTKLLTLDQLDDLFRLEIIDA
                     NTKVWQPGMPNWLPLSVVAGLEGDSEDEEETLCRPALSAGPPPPPMRRRTAATAPTIA
                     ASNLPAAPIAPSPIRAVPDQRAAAPRSLPPPPAAPPPAPRAAGRTAPIQAVPIHAVPG
                     NGYQAPAAGYPAAAGGQAVGPHGTQAMRPAVIPQAAPYPPAVQHAATLSAWPPAVHAG
                     APSARPAAVHANATIRPGVTAAMPAIVPAAIQAPESLRPLAVSVRPMALPEQPSSLGT
                     WVVMLAVCAGLGVSLYRNDVLRSLAARAGQEKSYLALESHVGAPGFGTPRAVEALPKP
                     FLEQGGVDTSGSGSSSLRDRGSSTRAQALAGDSPGEAKRNQEDFTSTKVASASSTNET
                     QVKSASDSGTGSAKEAVAAKPAAGMPTPPKASESHVEAAPKVRATARISAPTPEPAPK
                     KKEKATDSPAPSLGIRGSTNQFDPLNGKL"
     gene            8965..9747
                     /locus_tag="VFQ61_17835"
     CDS             8965..9747
                     /locus_tag="VFQ61_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956377.1"
                     /translation="MGRNPWQDDPDLAALRPRTGSTRWGRVFVGLVLVASATFAGAYY
                     LPLFQAHGTLTAEHRKVIDRAQELDRNLNRAQAELKKVTQRRDELEAERAKREAGVGR
                     SNAELEHSKADLANKLDKAIKRNWAQVATTDGHPLVGVADAAVFVPKKLEVSPAGKQL
                     LCTLAQAADNRELRVAAVDGNEPPAPQLVASYPNALALRAARAALIAFTLETECKVSG
                     ARLKAIAAGGASSTHSAFGASKLPDVHLEIEIVDAPTALASH"
     gene            complement(10090..>11049)
                     /locus_tag="VFQ61_17840"
     CDS             complement(10090..>11049)
                     /locus_tag="VFQ61_17840"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HET9956378.1"
                     /translation="PSGNDDVERNSLPNGLEVWGDPSDVWVAARRIWNQHAYHVTNVT
                     EGGQIPLHEPESWLPLNGRLYDTYRSQPRNYGVAPDLSPIEVQISSPDVACGKLSDEL
                     QISVLVKNLGDLRVGPGVDVAFTGHFEGPREDAALLDDQGQPIVVTLTQSLEPGSSTI
                     VSASYRRGNNGRDDLPAEIGVSVDARAAERECHEDNNEISGAVEAGAERADISLQLKS
                     ASGCLPATVQGTLTNDGSSPASDVLVRLYAGDPSQGGEPLAETTVAGPLAPGKTANFT
                     VTIDGLMRDLTLYGIADPLDTISECNDANNRAWGPSLPCDVVR"
BASE COUNT         1988 a         3483 c         3646 g         1932 t
ORIGIN      
        1 acccatgaag cgcacttcgg ggtaccttcg cgcgagcgtg cgttgttcag ggccgtcacc
       61 cagtacgacg atgtccgtga tgcccgcaag ccgtagagcg ttcagggcca cgtcgacgcg
      121 cttgctcgcg atcagacgcg aggcgatcac cacgagcggc gccggcgtga tcacgttgag
      181 ctgcttgcgg gctgcgctgc gcgagagtgg cacttcgacc tccagcgcgg gtggatgaac
      241 gtagatgcgc gcctcgagct cggctcgtaa agctcgtgcg gccgtggcgg cccgaaagtc
      301 cacagcttgc catacgatct gtgcgagctc ctccgagacg catcggaaat gtgcgccgcg
      361 ttggagcaag cccctcacga tcgaacgctg cacgggtcgg ggtaggcgag ccaacaatcg
      421 agcgtccgag ccgtgcacca cgcactcgag ctttgccacg gagttccgtg tgtaacgggc
      481 gaggggccag gcggagggga acaaccattg gcaaatgagc cgatcgaaag gaccatgggt
      541 ctttagccag cgccgcgcgc tccagagcca actcaataac ccgagcaagc gccacgggcg
      601 tgcgcggata cggggcaaca cgcccgggaa gccgcaggct ccgtgggcat cagccgaaaa
      661 cactgccgga tttgccgtct gcggatcggg atggcaccag ccgaactgcg ggtcgcgcgg
      721 gcaaaacaca ctgaccgaat ggccctggcg aaccaactcg agggcgtcgg cggcgacgaa
      781 atgccccgcc gcgtcaccat cccattgtgg ataggacgtg gtcaggatcg cgattcgcaa
      841 tgcctgcttc tcgactggct cgtctcgatg cgtgttcgcg gtgagcctct caaacgcgct
      901 ggagctcacc catggcgatc acctgggccg tgccctgttc atccactaga tcccccaggc
      961 tgcgcaggta tcggaacgag taaatgccgg aggaatgacc gtccccccac gtgaatgaga
     1021 gcgcgtagtt gcccacctgt tcgatctcgc gcagctcggt gttgccgccc gtctgaaact
     1081 tcagaccgct cgagtgccct tggcagccgg cgcaaggaca gtagcctctc agcagctcgt
     1141 tcgtgagccg ggatttgtgc ccgtcggccc acgttacttc cagttctttg ccgccgtggg
     1201 gagctttgac gttggtgggt ttgaagcgcg cctgttgcat gttcgcttgc ttgtttagtt
     1261 cgattcggcg gcgtggcgaa gcgcggttac tcgcgttcgc caagatccgt caggggtgcg
     1321 gcttgctccc taccagaatc cgcggccggc ggcaggcccc cgcgaccgtt gtacacgagc
     1381 tgccctgagc tcgcgaactc ccgaaatacc tcgagcgcct cccgcgaggc cagattgtcg
     1441 aaaacgcgaa tgccccgttt ttccaattct tctcgccact ggggatgaat cgggccctcg
     1501 tcgaaaccgg tgagctgctc catttccgcg ccgctgccgg caatcgccac ggatcgcaca
     1561 ccagcccaag ggatggcgcc gaagcacatg gcgcaaggac gccagctcgc gacgagctgg
     1621 tgagcaccta gctccggcgc gcccaaatcg aaacgaccca gagcggcttg cgctaggctc
     1681 aaggccatca cctcggcgtg cgcggacgag cacgctcggg gaaccacgcg attgacgccg
     1741 accgagacca gcgctccatc cgcctcccga aagaccgcgg cggcgaaagg ccccccgctc
     1801 gagttccgaa tgttttgacg agccagctcg agcaccagct gcacgcgcgc gtccaagctc
     1861 gtgagcgcga ccggcagctc cgcgagctgg ctctgcagcc aggccggcaa atccagggtg
     1921 atttggccga gcaagccggc gcgaggcggg tccgacttca tgaagtacct tcggaagtag
     1981 ttggtggccc gctgggccgg gtcagcgcaa ggatcaactc ctggtgctcg ggaaaccgtt
     2041 gctcgggaaa caggcgcaac aggtcactgc gggtcggcat ctcgaaatcg gagaactcga
     2101 aaccaggcgc tacgctacag ccgcaaagcg cgaagccgtg agcgctggcg gtgacacgcg
     2161 ccgcctgcca ctcgccggcg ggaatcacgt gttgcgggcg ctccccgagg gccaaagcgc
     2221 tccccaagcg cagccgctcg acgcccgctt cacggagcac gcacaactcc accgcgtctc
     2281 cgaggtagtg gtgccacgtc tcgtccgagc tcacccggtg aaaagccgag aaagagccgc
     2341 tctccagcaa atagtatatt tgcgtcgacg ccgagcgcgg gccgtcatgc gagtgactct
     2401 ggacccggtg aggtgagcga aagacctccc ggtaataacc accctctggg tgaggcgata
     2461 aattcaactc tcgaatgaca tcgcgcgcgc tgaccgtagc catcgcctcg aagcctatcc
     2521 caatcagcgc cagtccctaa acaaacgata cttgggcgct gcgttgggag gttacggtcc
     2581 gccaacaacg ccactcgacc gctcgattga agatacattc agtgcgttcg acgatcggat
     2641 tcgcttcgcg aacgacaccc gtgcgcaact cgaggcttgc cggaagcgct tgcgcgatgc
     2701 gcttgcgcga tgcgattgtg ccgctgggaa cggatctggt ccaccacggc gacgagaagc
     2761 tttgtcccgg caaagcgggt cgcgtgcttt gccgatgggg gcgagggtcg gccaacgcgg
     2821 tgcggaccac gacgaagtct gactcgccga cgtggcgatt gcgatttgct ttagtctcga
     2881 cgccctgaag atatcaggca ccgttggctc cagtttgtcc ttcaatccac aaattcatgg
     2941 gattgcgtcc tcggtcattc cgagacgggc cgagaagcgc taacctacgc cgcatacaaa
     3001 gcgaggagcg acatggcacg accgccgcac gaggcgaaca agccttcaga gtccgcaacg
     3061 agcgagccgc cggaggggca cgaacagaag ccattcgcgt cgctcttgcc gacgcgccgt
     3121 cagttcgtag tcggtgtgtc aggtgccgtc atcggcgtgg cttgcgccga atcccacgaa
     3181 gggggaggcg tccctacggg aaccggtggt accagcggag gcgtttcggc gggcggggcc
     3241 aaggcgaccg gcggggcacc ctcgaccggg gcagcgtctt cgacgggtgg agcagtatcg
     3301 acggctggag catccggtgg cctaacttcg acagctgacg cacggggcgg ctcaccttcc
     3361 acgggaggtg cagcgagctc gggtggtgcc gcgtccggcg gtgttccggc gggtggagcg
     3421 gcttccggcg gggttccagc cggcggagcg gcttccggtg gggttccagc cggcggagcg
     3481 gcttccggtg gcaccagcgg aggcgtgact cgcaagttgg cgagcgacca ggtcgtcttg
     3541 ggcgcgaccg gcatcaaggt gtctcgtcta gcgatgggtt gcggatccca cggctccaac
     3601 aagggctcgg atcaggcccg cttgggggtg gatgcgttcg ccggactgct caagtacgcc
     3661 tacagccaag gcatcaactt cttcgagacc gcggacgctt acggctcgca tccccacgtt
     3721 gcggaggcca ttcgtcaggt ggggcggaac aacgtcgtgg tcctaaccaa aaccatgtcg
     3781 cagcgggccg ccgacgttga ggcggacttg gcccgcttcc ggaccgagct cgggatcgat
     3841 gtgatcgaca tcgtgctcct gcacctgaag accagctcgc gctgggccac cgactgtgag
     3901 ggagcaatgg aggtgctgag caaggccaag gagagcggcg cgattcgagc gcacggcgtc
     3961 tcgtgccata gcttgcaggc gttgcgcctg gccgccgcca cgccctgggt ggacgtcgat
     4021 ctcgcgcgca tcaacccggc gggtgtagtg atggactcgg acccgaccac ggtgatcagt
     4081 gtgctccgcg agatgaagac cgccaacaag ggcgtgattg gcatgaagat cctgggtgaa
     4141 ggccagctcg cggaccggct ggatcaggcg atccaacacg cggtgggcct cgactgcatc
     4201 gacggcttca cgattggctt cacgagccag gctcagctcg atcaggtgat tcagaagatc
     4261 gccgcggtct gacggcgcga cgggctccta ttggaatagg ttcttagcaa ggcgccccca
     4321 agccgcgctc gaactcgcca aacggatcga gcagcgccgt cacgatccag cgcacaccgg
     4381 gggagtgaat cgatcgggct gcgtgctgac cccagtaacg agcttccccc agcgcgaggc
     4441 ccgctatggg cgtgtatacg aggtccaaag ccgaaggacg aacgccgttc gcttcgaacc
     4501 cgtactccca gacggccgag gcggacgtcg cgaacaagag cgccccccaa aaaccagagt
     4561 tgcagcgccg ggctcgatag aagagctcgg aaccgagcag cccgtggccg aaaacattga
     4621 tggtgtatcg atcaccgtcc cattcgaagg ctcgacgatc agggtgaaat actggcggtt
     4681 gcgtgaaggc gtcggcgtag tgttttcccc atttcgcggg cgactcggcg aacgggctcg
     4741 gccacaaata ggcctcggtc agacgcattc ccgtaaatag cgccgccgtg tgcgccacag
     4801 gcacccagag cagaggctca gtagtctcag cgcctggact ccgatccggg ttgtggcccg
     4861 cgattggagc tgggccactt ggggttccag gcgtacttcg agcggtagat tggggctcgg
     4921 agaatccgac tcgcgctggc tgctcgccga cgattaccgc ggtcgatgcg gcctcggacg
     4981 cggcttgtgc gcctgcaggg agggcatgag ctagggcggc tacacacacc aggggcctcg
     5041 acaaagcacg aaaaatccgc ccaatgcgcg gctttggggg tggatgggat ccgggctcgg
     5101 gcagcccatt cgaggccaag cgacgcagca cgacgaaagc cttgcccccg agaaaaaacc
     5161 atgtcaataa gccgctccct tggcgaaccc tagcgaagcc gtaacgtgta agccactcga
     5221 ggtccaagtc ggagacaagg gcatcgagcg ggcaatcaaa catctcaagc gccgtatggc
     5281 tgcggagggc atccttcgtg agctcaagcg tcggcgccac tacatgaagc cgtccgtgaa
     5341 gcgccgcaag aagatgagcg aagctgcgcg tcgccgccgc aagcgcttgc gcgcggacga
     5401 gcagcaacgc actggcatgc tgtagatccg gctggcctaa tcccggcgcg catagcgttt
     5461 gacgtatctc acgtcaccga gagcgtgccg ggattgctcg atacgcccgg attgctcgat
     5521 acacagcgca gttatttttg ccaatgattt ggcccaagtt ctgttgagcg tttagcttcc
     5581 gggcggtgtc ttcgcccaag tatccggtgg cccgctcctc gtaccgggga cacgcgccga
     5641 gaacgctctc aggaccacag cagtcccaac tgtccgatcc gcggcagcca cgccgccaga
     5701 cggacgcccg gaacgccgct tggcaatccg aatcggtccg catcgcgaca gcagggctgc
     5761 gggataccgc ggcgccgcgc gaggcaaacg tgcgcggacc aggcgcggcg accctgcgca
     5821 gcttgcgaac tcaagctggc aagtcgtctc agccgggtcg gcttgcgaaa ggctcgtcac
     5881 gaggagcgaa cggggccggt gccggcggcg atcacgctgc ggatgacggc ggcgtcggtg
     5941 gaacgtccgc gccgttgatc cgtctgcgtt acgtgcttgg agcgctcggt gcaattggca
     6001 tgatcgtgtg gggaattccg cgcgcgcgtg ccgcctggga cgttcactcc gtgggctcca
     6061 cccttgcgga ttatgcgttg tgcatggctg gccccactgg gccagagttg atccgcgatg
     6121 acgacgcgga atttcggcgc ttgatccggc ggcgtttgct cgcggccggg cccgaggacg
     6181 cgccgttcgc acgttgcggc aagctcgcgg tcgagctctc gtccaaggcc gagttggccc
     6241 aggttcacgc gaacaaggcg caggccttcg aagagtacgg cctgaatggg ggacgagtgc
     6301 cgctcaccgc gttgggtgta gacgcgggcg tgctgcagcg tcgcgcagag caagcgtggc
     6361 cgttcgttcg tggcagcagc gcgcggctcg tgcgcccatc gttgggtgcc gtagaggcag
     6421 ttcatcccgt tcctccgccg cgcccgagct tgggacgcgg tctacccgcg acccgcactt
     6481 tgccgcgcaa cacctggcaa actcgcgagg cactgtttct gtcggtgggg aacggcgttg
     6541 ggcaaagctt gtacttctcg agcgacgcgg gagccacctt ccgcgcatcg cgcagcgcac
     6601 cgcaggcaga ggcttatgca gagggctgcg tcggcaaaga tccgagccat ccattcatgc
     6661 tcagcatcgg tgaggacggc tccttgcggg tgggctcgaa ttcggccgat cagggcagct
     6721 tcagttcgac cgctgtgcaa ggcgagcacg agctgctcgc attggcctgt gacgactcgg
     6781 ctttggtcgt cgcggcacgc cgtgaagggg cacaagctgc gcagttcgtg ttgtgcccgc
     6841 agctccggag ctgcagcccg ttgccgttgc ccaagaccgc gcctttcact tcgctggcgg
     6901 gctctgactt cgacgtggct cgtagcgcag gcgtgacggt gttgatcacc gccaaccgtg
     6961 gcgtggttcg cgtggtctct tcgagggatg acgggcaaac gtggactcca cagagcgtgg
     7021 ctttcgatgc cttcgaacac ccagcgctcc gtgccgacgt gaccgtaccc acgcggctgc
     7081 tcagcgctgg cgggcgcctg tttctatatg gcgccaccag caagcccaac caaagctatc
     7141 cagtgctcgt ctcagacgat cagggcgcaa gcttccgtgg gatcggcgct agccccgagg
     7201 agccgggccg tgcacgtgtc gcgacggctc cgcgtcgttg accgccgcgt cgcgatattg
     7261 accgccgcgt cgcgatgttg atcggctcaa tcagtcagag ccgctccggt tcggagtagc
     7321 cgtccgattc gcgcccctgg tttagagctt cccgttcaac gggtcgaact gattggtcga
     7381 gccacggatt cccaagctcg gcgcggggct gtccgtcgcc ttctccttct tcttcggagc
     7441 gggctcgggc gtcggcgcgc tgatacgcgc cgtggcgcgc accttgggag ccgcttccac
     7501 gtgcgactcg ctggctttgg gcggtgtggg catccccgcg gcgggtttgg ccgcgactgc
     7561 ctctttggcg ctgccagtgc cagagtcact tgcgctcttc acctgggtct cgttggttga
     7621 ggaggccgag gctaccttcg tggacgtgaa atcctcttga ttgcgcttcg cctcgccggg
     7681 cgagtcgccc gcgagagctt gcgccctcgt gctggagcct cggtctcgca ggctcgagct
     7741 gccactgcca gaggtatcga cacctccttg ctcgagaaac ggcttgggca gcgcttcgac
     7801 cgcgcgcggc gttccgaacc cgggggcgcc cacgtgagac tcgagcgcga gatagctctt
     7861 ttcctgaccg gcgcgcgctg cgagcgatcg cagaacatcg ttgcggtaca gcgagacacc
     7921 cagccccgcg cagaccgcga gcatcacgac ccaagtccca agactcgacg gctgctccgg
     7981 caacgccatg gggcgcaccg agaccgcgag cggccgcagg ctctccggag cttgaatggc
     8041 agcgggaacg atcgccggca tggccgccgt cactcctgga cggatggtag cgttggcgtg
     8101 cacggccgcc ggtcgcgcgc tgggtgcgcc agcatggaca gcgggcggcc acgcactcaa
     8161 ggtcgccgcg tgctgtaccg caggcgggta cggcgctgct tgcgggatga ctgcgggacg
     8221 cattgcctgg gttccgtgag gcccaacggc ttggcccccg gcagccgccg gatagcctgc
     8281 ggcgggtgcc tgatacccgt tgccaggaac tgcatggatc ggcaccgctt gaatgggagc
     8341 cgtgcgcccg gcggcgcgag gtgcaggagg cggagccgct gggggtgggg gcagcgagcg
     8401 aggggcggcg gctcgctgat ccggaacggc acggatgggc gatggcgcga ttggagccgc
     8461 ggggaggttg cttgcagcaa tcgtcggcgc ggttgcagcg gtacgacggc gcattggtgg
     8521 aggagggggc cctgcggaga gcgctggacg acaaagcgtc tcctcctcgt cctccgagtc
     8581 gccttccaac ccggcaacca ccgaaagcgg cagccaattc ggcattccgg gctgccaaac
     8641 tttggtgttc gcgtcgatga tctccagacg aaatagatcg tcgagctgat cgagcgtgag
     8701 aagcttggtg tccccgggcc caatagcaac atgccaaagg tcttcgggaa cttgggccat
     8761 atagtttggc tgagccacca tgtgggcgat ctgtagcagc cagcacggct ggccgcacgc
     8821 taatcattat gtgtctcgtg cacctgacgg cgggtcatgc agcgcgcatg tcaggctctg
     8881 ctcaaactga aaagttcctg tgcgaatcgg gtttttcgcg ttcgggccta gcccaatcgg
     8941 cttctgccgg ctacctgaag gggcatgggg agaaacccct ggcaagacga tcctgatttg
     9001 gctgctctgc gaccgcgtac cggctccact cgctggggcc gtgtcttcgt agggctagtt
     9061 ctggttgcga gcgcaacgtt cgcaggggct tactatttgc ccttgttcca ggcgcacgga
     9121 acccttaccg ccgagcaccg taaggtcatc gatcgagcgc aagagctcga tcggaacctg
     9181 aatcgggcgc aggccgagct caagaaagtc acccaaaggc gtgacgagct cgaggccgag
     9241 cgcgcgaagc gcgaggcggg agtggggcgc tcgaacgctg agctcgaaca cagcaaggcg
     9301 gacctcgcga acaagctcga caaagcaatc aagcggaact gggcgcaagt cgcgaccacc
     9361 gacggacatc cattggttgg cgtcgcggat gccgctgtat tcgtgcccaa gaagctcgag
     9421 gtcagccccg cgggtaagca attgctttgc acgctggctc aagcagccga caatcgcgag
     9481 ttgcgggtcg cggcggtgga cgggaacgaa cccccggctc cacagctcgt ggcgagttac
     9541 ccgaatgcgt tggccttgcg tgcggcccgg gcggcattga tcgccttcac gctcgagaca
     9601 gagtgcaagg tgagcggcgc tcggttgaaa gcgatcgcgg ccggtggcgc ttcgtcgacg
     9661 cacagcgcct tcggggcaag caagttgcca gacgttcacc tggaaatcga gattgtggac
     9721 gcgcctacgg ctttagcgtc gcattgactt tgcgtgttag ctcgggcggt gttggctcgg
     9781 gtgctgttga gaccaggagt gtaggaacaa ggatatcgaa tcgagccgaa gcccgattcg
     9841 atatccttcg gtcgaatgcg ggccgcaacg acacggaggg gcaggcccgc gggggcggcg
     9901 ggttcagctc gtgttcgttg cggtcctgaa tggagtccta catcagacgt cgtgtgttgc
     9961 aaactaaata acgttcgcac atgcggtatt tgtctcgtgc gcgcacggga tatcccacta
    10021 tccgtgtctg aaaccgtgga ttcgcaattg ccatgttcgg gttcacccgg gattccgggc
    10081 gaacccggtt cagcgcacta cgtcgcaagg caagctcgga ccccaagcac gattgttcgc
    10141 gtcgttgcac tccgagatcg tatccagagg atccgcaatc ccgtacagcg ttaggtctcg
    10201 catcaagcca tcgatggtaa ccgtgaagtt cgccgtcttt cccggcgcca agggccccgc
    10261 gaccgtagtc tcggcgagtg gctctccgcc ttggctcgga tcgcccgcat agagtcggac
    10321 cagaacgtcc gaagctggag acgaaccgtc gttagtgaga gtcccctgaa ccgtagcggg
    10381 aagacaacca ctggcactct tcagttggag gctgatatcc gctcgttcgg cgcccgcttc
    10441 gaccgcaccc gagatttcat tgttgtcctc gtggcattcg cgctcggcgg ctcgcgcgtc
    10501 cacgctgacg ccaatctccg ccggcaagtc atcgcgtcca ttgttcccgc ggcgatagga
    10561 cgcgctcacg atggtagaag agccaggctc cagactctgc gtcagcgtga cgacgatggg
    10621 ctgtccttgg tcgtcgagca acgccgcatc ctcgcgagga ccctcgaagt ggcccgtgaa
    10681 tgccacgtcc acgcccggcc ccacccgcaa gtcgcccaga ttcttcacca acaccgagat
    10741 ctgcagttca tcggaaagct tgccgcaagc cacgtccggt gaagagatct gaacttcaat
    10801 cggcgacaga tccggtgcca cgccgtagtt tcgcggctgt gagcggtagg tgtcgtaaag
    10861 acgcccattc agcggcaacc aactctcggg ctcgtgcagc gggatctgcc ctccctccgt
    10921 gacgttggtc acatgataag cgtgctgatt ccagatccgc cgcgcggcta cccacacatc
    10981 gctcgggtcg ccccacacct cgagcccgtt cggcagtgaa ttgcgctcca cgtcgtcgtt
    11041 gccgctcgg
//
LOCUS       DASTKU010000515         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_321152, whole genome shotgun sequence.
ACCESSION   DASTKU010000515 DASTKU010000000
VERSION     DASTKU010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321152"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VFQ61_17845"
     CDS             complement(<1..1116)
                     /locus_tag="VFQ61_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956379.1"
                     /translation="MKSKSRRQGRGKLAPFMTSVCGIVTFACSATDPTAGAPAEFAAE
                     QGVAVASTTQKVVPDYALPTTLSPIRALEAPSGVYLTSVEQRLDGTGTFTQTVHVYQP
                     DANSETIRFELTAKNSTGQVDGSHRFESVVLTSHPGESDQSQQTLANASGQRVYEITN
                     SRQGWYRLQFRYVSPNFGRTLSIKAYDAGARPLKMAWSDPPSTRVQVRANVNVEQSSQ
                     SFLKGGAYRSADRLHSAYADNVTVDGRLLYRRAFDQGLFNYPLGWLSAGSHVVEFSLS
                     ASNYSRAKFWFGVNEKTFHSFQAQDAWKAAQFLYYGEMHTADYDAWNEGVAFAQGAEL
                     ANGVRPPPVRRGTSFGLALEHPGLNGSTANASLRIYPY"
     gene            complement(1425..2690)
                     /locus_tag="VFQ61_17850"
     CDS             complement(1425..2690)
                     /locus_tag="VFQ61_17850"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9956380.1"
                     /translation="MWCRRSGWLALLLLQAAACEKDDDAKPLAQGGASGNSARNTGGA
                     QTTAGATARGGGSGSTGSGGAAMSTGGVGSTGGQRDGSGGTDNSTLGGSAGAHGGVAN
                     TESEGGATGGAGGETGETSTAGESGAGGADPGSEPPSCRGLPESCGSGAQDCCSNTQV
                     PGGTFNRSNDVAAPATVSAFNLDVFEVTVGRMRRFVEAYPGSRPSAGSGKNPHDAGDP
                     GWNLEWDRYLPASRSELIGSTGLSCGSLQTWTDEPGDNENRPIVCATWYVAYAFCIWD
                     GGRLPTEAEWNFAASGGSAQLRYPWARSSKEQTLDATYANYVDSNHCVPNCSAKDLFP
                     VGSLPLGIGRFGQLDLAGNVREWVADVFSENYPTPCVDCAYRGAGPYRVLRGGSWGDT
                     PNNLLTSARSSDLPEYHPGNLETGFRCAR"
     gene            2852..>3982
                     /locus_tag="VFQ61_17855"
     CDS             2852..>3982
                     /locus_tag="VFQ61_17855"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9956381.1"
                     /translation="MSCALAAVLTLSSLACSSDDGGPSNGSAGQGGRVNEGGNSNSRG
                     GSSGTGGSTRGTAEAGSSGRGDPDRGGAGGASNRGGESSVGGSAAGASAAGGSAAGGS
                     AAGGSAARGGSSSVSGGAGGSAGTDDTTSAGAGGSEGEVDACADARPTPLSCDEDLSC
                     WTQLEFKLNSYSVGNTPNGVAVGDVNGDRWPDVVVSNGEASDVSLLLGKKDGTLAAAV
                     SLPVSSGRENEPGHPLSVALSDINRDGHLDVLAAHDWMLGVGILLGRGDGTFEPVYSV
                     LPDGGHDNMTLADMNHDGDPDLLFVATGGDYVSVALGNGDGTFGDAQRFNTWAYPAGL
                     AVADVNTDGDLDVIVGHTVSRDVVVLLGFGDGRLQPLNTFQAN"
BASE COUNT          726 a         1234 c         1262 g          760 t
ORIGIN      
        1 gtagggatag atgcgcaagc tcgcgttcgc ggtgctgcca ttgagcccag ggtgctccag
       61 cgcgagccca aaagacgttc cacggcgcac gggtggcggg cgaaccccgt tggcgagctc
      121 cgcaccctgc gcaaacgcga cgccctcgtt ccaagcatcg tagtctgccg tgtgcatctc
      181 gccgtagtac aagaactgag cggccttcca ggcatcttga gcctgaaagc tgtggaaggt
      241 cttctcattc accccaaacc aaaacttcgc gcggctgtag ttgctcgcgg agagcgagaa
      301 ctccaccacg tgcgaccccg cgcttagcca acccagcggg taattgaaca agccttggtc
      361 gaaggcgcgg cggtatagca ggcgaccgtc caccgtgacg ttatcggcat aagcagagtg
      421 caggcgatct gcgctccgat acgcgccacc tttgaggaag ctctgtgaag actgctccac
      481 gttcacgttg gcacgcacct gaacgcgcgt gctcggcggg tcactccacg ccatcttcaa
      541 cggcctcgct ccggcatcat aagccttgat gctcagcgtg cggccgaaat tgggagagac
      601 gtacctgaat tgcaggcgat accagccttg gcgtgaattc gtaatctcgt atacccgctg
      661 tccgctagcg ttggcgaggg tttgctggct ttggtcggat tcaccgggat gcgacgtgag
      721 caccacggac tcgaacctat ggctcccgtc gacctgaccc gtcgagttct tggccgtcaa
      781 ttcgaaccga atcgtttcgg agttcgcgtc cggctggtaa acgtgaaccg tctgcgtgaa
      841 cgtgcccgtg ccatccagtc gctgttcaac ggaggtcaaa tacacgccgg aaggtgcctc
      901 cagcgcacga atcgggctga gcgtcgttgg cagcgcgtag tcaggaacta ccttctgcgt
      961 cgttgacgct actgctacgc cctgctctgc agcgaactct gccggcgctc cagccgtagg
     1021 gtccgtcgcc gagcaggcga aggtgactat cccgcagaca gatgtcatga agggcgccag
     1081 tttgccgcgc ccttggcgtc ttgattttga tttcatatcg tggcgattag agtcaccccg
     1141 taggtagagc ttcaccctcc ccgtccgatt ttcggtgcag cccctcggga agccctcatc
     1201 gatcagcagg gcgcgcgccc gccaccgaag gccgttttcg ggaggccagc tcgagaggct
     1261 cttcgcaaac tcgaacacac ctctcgaaac cgccaccgca acccgagctg tcctggatca
     1321 tggtcgagcc ggggatgccc gactattggg cttagagccc caaaacagag ccgattgggc
     1381 ctagtcgccg tctcatgcgc tcctctaaat gttcttgccc aatctcaacg cgcgcagcgg
     1441 aagcccgttt ccaagttgcc agggtggtac tcgggcaaat cgctacttct cgccgaagtg
     1501 agcaggttgt tcggcgtgtc accccagctc ccgccgcgca gcacgcgata ggggccggcg
     1561 ccgcgataag cacaatcgac gcagggagtt gggtagttct cggagaacac gtccgcgacc
     1621 cactctctga cgttgccggc caagtctagc tgtccaaaac ggccgatgcc gagcggcagc
     1681 gagccgacgg gaaacaggtc ttttgccgag caattcggca cgcagtggtt cgaatccacg
     1741 tagtttgcat aagtcgcatc cagggtctgt tccttggatg agcgcgccca cggataacgg
     1801 agttgagcgc tgccgccgga cgccgcgaag ttccactctg cttcagtggg tagccggccc
     1861 ccgtcccaga tgcagaaggc ataagcgacg taccaggtag cacatacgat gggtcgattc
     1921 tcgttgtctc ccggttcgtc cgtccaagtc tgaaggctgc cgcaactgag cccggttgag
     1981 ccgatcagct cgctgcgact cgcgggtaag tagcgatccc attcgaggtt ccaccctgga
     2041 tcacccgcat cgtggggatt tttgcctgat ccagcgctcg ggcgactccc gggatacgcc
     2101 tccacgaagc gccgcatgcg accgacggtg acctcgaaca cgtctagatt gaaggcgctc
     2161 acggtcgcag gagcagcaac gtcgttgctg cgattgaagg tgccgccggg aacttgcgta
     2221 ttggagcagc aatcctgcgc gccactgccg caactctcgg gcaaccctcg gcagctcggc
     2281 ggctcggagc cggggtctgc tcctccagcg cctgattctc ctgcggtgct cgtttcaccg
     2341 gtttctccgc ctgcgccacc ggtggctccg ccttcactct cggtattggc gactccgcca
     2401 tgggcacctg ccgacccacc gagggttgaa ttgtccgtgc cgccgctccc gtcacgctga
     2461 ccaccagtag acccgacacc gccggtcgac atcgcggcgc caccgcttcc ggtcgacccg
     2521 ctcccgccgc cgcgagcagt tgcgccggcc gtagtctgag ctccgccggt gtttcgggct
     2581 gagttaccgg atgcgccgcc ttgggcaagg ggctttgcgt cgtcgtcctt ctcacaggcc
     2641 gcagcctgca agaggagtaa agctaaccaa ccggatcgcc tgcaccacat acgttcaccc
     2701 cgttcgattc agatgagctc cggagcattc caccgagccc tcgattgagt caagcaggac
     2761 gtttctaacc gcagttgctg acgcgggcga gtggttgctc tatgctgcgt cgatggtgtg
     2821 tagtccgaag gtgtgctcaa tacgcgcttc gttgtcgtgt gcgctcgcag cagtactaac
     2881 cctctcgagt ttggcttgct cgagtgatga cggcgggccg tcgaatggct ccgccggcca
     2941 gggaggccgc gtcaacgaag gtggtaattc caattcgcgc ggagggagtt cgggtacggg
     3001 cggcagcacg aggggcaccg cggaggccgg gagctcgggc cgcggagacc cggaccgagg
     3061 cggcgccggt ggagctagca atcgaggtgg cgagagctcc gttggcggca gtgccgcagg
     3121 tgccagtgca gcaggcggca gtgcagcagg tggcagtgca gcaggtggca gtgcagctcg
     3181 cgggggttcg agctctgtca gtggcggtgc gggcggctcc gccgggacgg acgataccac
     3241 gagcgcaggc gccggcggct ccgagggcga agtagacgct tgcgcagacg cgcggccaac
     3301 accgctgtct tgcgacgaag atctgagttg ctggacgcag ctcgagttca aactaaacag
     3361 ctattccgtg ggcaatacac cgaatggcgt cgccgttggg gatgtgaacg gagatcgctg
     3421 gcccgacgtc gtcgtatcca acggagaggc gagtgacgtc agcctcttgt tgggtaagaa
     3481 agacggcacg ctcgccgccg cggtcagcct gcccgtgagt agtggaagag agaatgaacc
     3541 ggggcatcca ctctcggtcg cgctgtccga tatcaatcga gatggccact tggacgtcct
     3601 ggcggcgcac gattggatgc ttggagtggg gatcttgctg gggcgtggcg acggtacgtt
     3661 cgaaccggtc tattcagtgt tgcccgacgg cggacacgac aacatgacct tggccgatat
     3721 gaatcatgac ggtgacccag atctgctgtt cgtggccacc ggcggagatt acgtcagtgt
     3781 cgcgctcggc aacggcgatg ggacgttcgg cgacgcgcag cgcttcaaca cctgggctta
     3841 tccagctggc cttgccgtag cagatgtgaa cacggacggg gacttggacg tgatcgtggg
     3901 tcacaccgtg agccgggacg tcgtggtgct gctcggattc ggtgacggac gcctgcaacc
     3961 cttgaatacg tttcaggcca ac
//
LOCUS       DASTKU010000516         6428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_544987, whole genome shotgun sequence.
ACCESSION   DASTKU010000516 DASTKU010000000
VERSION     DASTKU010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6428
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544987"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1852
                     /locus_tag="VFQ61_17860"
     CDS             <1..1852
                     /locus_tag="VFQ61_17860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9956382.1"
                     /translation="PELVVGRNDRELFSNEEALAIQRNDRRILQDAVTRTYEEVISTA
                     AGPMTLLATKGPLLDARGNVVGTFGISRDITDRKRGEALLQESRAKLENVIESLNEGL
                     IIWDVETGEVHWNRAALLMHGFDGKQQPNMNVSTLQRSFQLTTLDGQAVSFYDWPLNR
                     VLRGESVSGLSLQVRRPELDLERIFTFNGSLARREDNQPMFALLSINDVTLSRRAEES
                     ARHAQRLEALGTLSAGVAHDFNNILFAIAGNVQLARLEVPESSPALEFLDEIGRASAR
                     AADLVRRILLFARSQESRRSAVELWPVVEDALKLLRPTLPAQIQITTSVADPGAVVRA
                     DTSQIHQVITNLVTNAAHAVLRAERPAPRQVRILLESIALTRELPCHGRSLQPGRYAH
                     LTVEDDGCGMDEVTARRAFDPFFTTKPTGEGTGLGLSIVHGIVSNHEGAVGIESERGK
                     GTRVHVYLPIATEAVVRVAAQGSSITRSKLSVLFIDDEQALVRVAERGLGRLGHRVAG
                     FVSAQEGLRSFEREPDGFDVVVTDLSMPELSGFDVAETIRSRRPGLPILMMSGFVRPE
                     DRERARQLGIRELLVKPSSIDQLAAAIDRCCAPALLEGAQRAPGHASGGA"
     gene            complement(1816..4017)
                     /locus_tag="VFQ61_17865"
     CDS             complement(1816..4017)
                     /locus_tag="VFQ61_17865"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9956383.1"
                     /translation="MRNWFGFGSGSAVLAAVAVVSCVGSKTQPAASGGRAPTDAGARE
                     SSLIATDRTQTRAAHHFTARAHEPHYPAAVAALPPLASMPKFVDGSAQCFPQPPVVYQ
                     SPPYPTRYRGGAGTGAGIAGGALVSGGSAQPAKPKPSRALPSSPVAEGAAPGTASKDA
                     YAAAPPPAAAAPAPPALAPPPAPAAAAPGDGRADSAASKKAPEAKRERAPEPRQTASE
                     FDDRRKGDLEVAVPPPARQREPEWGAGIYLSNDDTMSLSSAQRVLWAIDHYAPIPQEH
                     VRPHELLNYFSFDTRPVERDHDFSVLPHIEPSPENPNLATLALSVRGRPATTETRRNV
                     NLAYVVDRSGSMQAEGRMEYLKRGLLRSLKELKQGDIVHVTLFDTNACDLVQNFVVGR
                     DPLSRLEQLIRRIEPLGSTNLHDGLNRGYASVDRSYQPTYSNRVVLVTDALANTGVTD
                     PVLLATVAKHYDDRRVRLSGVGVGTEFNDSLLDGLTEAGKGAYVFLGSEAEVDAVFGS
                     RFVSLVETVANDVHFKLQLPPSLAMQTFYGEEASTVKERVQAVHYFANTSQLFLSDLV
                     SRDGRLPTQDDILFTIEYQDPESGAARVEEFAWNLGEIAGRAPNLDKARLVSLFARSV
                     GSVAERPLPAGYTRRSGGWADVEAVGQCQTVRGELARLAVGIAADREVTRVQGLWDQL
                     CSRYAYQPPPPPVARPQTAPPPNDWPSASSPHSPPVRNNDYAPPDAWPGAR"
     gene            4326..5081
                     /locus_tag="VFQ61_17870"
     CDS             4326..5081
                     /locus_tag="VFQ61_17870"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HET9956384.1"
                     /translation="MQAHDMDEVIALMNGVVAGYRDRRSPRALFAAMYRRVTLRVQSA
                     IAGGAFVDGPRMDGLATEFANRYFAALEAIEHDRRLARAWQIAFDAEARSHTMILQHL
                     LLGMNAHINFDLPIAALAVTKDGNLADLESDFYAITGVLVELLDPVQAVVNRFSPLLD
                     VLDRVGGRTDETIVTFSISNAREEAWHEANRLAGEPPVLRERSLLSLDRRVALLAERI
                     ILPGGPCGLATDLIARTEVADIPVIVDALTAIP"
     gene            5183..>6428
                     /locus_tag="VFQ61_17875"
     CDS             5183..>6428
                     /locus_tag="VFQ61_17875"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET9956385.1"
                     /translation="MAHETRKEQDSFGPIDVPADRLWGAQTERSLRFFAISNERVPRE
                     LVRALALVKKAAADVNVDLGILELRIGEAISRAAEEILAGAHDGEFPLSLWQTGSGTQ
                     TNMNLNEVIANRASELLGGVRGPGRLVHPNDHVNLSQSSNDVIPTAMAIAANRAVRDE
                     VIPKLTELTNSLTVKAGALQGVVKIGRTHLMDATPLTLGQELSGWVAQLRHGLLHLDS
                     TLDHLSELALGGTAVGTGLNSPPHFSERVAARLAELTGYPLITAPNKFEALAAHDALV
                     FAHGALKTLAASLFKIANDVRWLASGPRSGIGEIVIPENEPGSSIMPGKVNPTQAEAL
                     TMACVRVFGNDTVVNFAGASGNFELNVYKPVIIDAFLQSARLLADGADSFRKHCVEGL
                     APNRARIAHHLEQSLMLVTALSP"
BASE COUNT         1140 a         2019 c         2091 g         1178 t
ORIGIN      
        1 acccgaactc gtggtgggcc gtaacgatcg cgagttgttc tccaatgagg aggcgctcgc
       61 catccaacgc aatgaccgga gaatcttgca ggatgcggtc actcgaacgt acgaggaagt
      121 aatctccacc gcggccggcc ccatgacgtt gttggccacc aaggggccgc tgctggatgc
      181 gcgcgggaac gtggttggaa ctttcgggat ctcccgggac atcacggatc ggaagcgcgg
      241 tgaagcgctc ttgcaggaga gtcgagccaa actcgagaac gtgatcgagt cactcaacga
      301 gggtctcatc atctgggacg tcgagacggg cgaagtgcat tggaaccgcg cggcgctcct
      361 gatgcatggc ttcgacggca agcagcagcc gaacatgaac gtgagcacgc tccagcgcag
      421 cttccagctg actacgttgg atggccaggc ggtcagcttc tacgattggc cgctcaatcg
      481 agttctgcgc ggcgagagcg tgagcgggct ttcgcttcaa gtgcgccgcc ccgagctcga
      541 tctggagcgg atctttacct tcaatggcag cctggcgcgt cgagaggaca accagccgat
      601 gtttgcgctc ctgagcatca acgacgtcac gctgagccga cgtgcggagg agtcggcgcg
      661 ccacgcgcag cggctcgaag ctctgggaac tctgtcggcg ggggtcgcgc acgacttcaa
      721 caacatcctc ttcgcgatcg ccggcaatgt acagttggcg cgtctggaag tgccggagtc
      781 ttcacccgct ctcgagttcc tcgacgagat cggaagagcg agcgcccgcg ccgctgactt
      841 ggtgcgtcgg attttgctgt tcgcgcgctc ccaggagtcg cggcgaagcg ccgtagaact
      901 gtggcccgtg gtcgaggacg ctttgaaact cctgcgtccc accttgcccg cccaaattca
      961 aatcacgact tccgtggctg atccgggcgc agtcgtgcgc gcggacacct cccaaattca
     1021 tcaagtgatc accaacctgg tgaccaacgc cgcgcacgcc gtcttgcgcg ccgagcgccc
     1081 cgcaccccgg caagtccgta ttcttctcga gtcgatcgcg ttgacgcggg agctaccgtg
     1141 ccacgggcga tcgctgcaac cgggtcggta cgctcacttg acggtcgagg acgacggctg
     1201 cggcatggat gaagtgaccg ctcggcgcgc cttcgatccc ttcttcacaa cgaagccgac
     1261 gggcgagggg acgggcctcg ggctctcgat cgttcacggc atcgtgtcca atcacgaagg
     1321 cgccgtgggc atcgagagcg aacgcggcaa ggggacccgc gtccacgtct acttacccat
     1381 cgcgaccgag gccgtagtgc gggtcgcggc gcagggctcg agcatcactc gcagtaagct
     1441 cagcgttctc ttcatcgatg acgagcaggc gttggttcgc gtcgcagagc gtggcctcgg
     1501 ccggctgggg caccgggtgg ctggtttcgt gagcgcccag gaaggtttgc gttcgttcga
     1561 gcgggagccc gatggcttcg acgtggtggt cactgacctg tccatgccag agctgtcggg
     1621 tttcgatgtc gcggagacca taaggtcacg ccggcccgga ctgccgattc tgatgatgtc
     1681 gggcttcgtt cgaccggaag atcgcgagcg cgcgcggcaa ctcgggattc gagagctgct
     1741 cgtgaagccc tccagcatcg accagctggc ggcagcaatc gatcgttgct gcgcgccagc
     1801 cctgctcgaa ggcgctcaac gtgcgcccgg ccacgcgtcg gggggggcgt agtcgttgtt
     1861 ccgcaccggg ggcgaatgag gtgagctcgc cgagggccaa tcattcggcg gcggtgcggt
     1921 ttgcgggcgt gcgacgggtg ggggtggcgg ctggtacgca taccgactgc acagctgatc
     1981 ccatagacct tgaacgcgcg tcacttctcg atccgcagcg atgccgaccg ccagtcgagc
     2041 cagctcaccg cgaaccgtct gacattggcc aacggcctcc acgtctgccc atccgcctga
     2101 gcggcgcgtg tagcccgcgg gcagaggacg ctccgccacg ctgcccaccg agcgagcaaa
     2161 caagctcacg agtcgagcct tgtcgagatt gggagctcgc ccggcgattt cccccaggtt
     2221 ccacgcgaac tcttccacgc gggccgcgcc gctctctgga tcttgatact cgatggtgaa
     2281 cagaatgtcg tcttgggttg gcagcctgcc gtcgcgggac acgagatcgc tgaggaagag
     2341 ctgggaggta ttcgcgaagt agtgcacggc ttgtacgcgc tctttcacgg tggatgcttc
     2401 ctcgccgtag aacgtctgca tcgcgagtga aggcggcagt tggagcttga agtgcacgtc
     2461 gttggccacg gtttcgacca gcgaaacgaa acgggagccg aacaccgcgt ccacttcggc
     2521 ttcactgccc aagaacacat acgccccttt gcctgcctcg gtcaggccat ccagcagcga
     2581 gtcgttgaac tcggtgccta cgcccacgcc gctcaagcga acccgccggt cgtcgtagtg
     2641 cttcgctacc gtggcgagca atacggggtc ggtcactccc gtattggcca aagcgtcggt
     2701 gacgagcacc acacggttcg agtaagtggg ctggtagctc cggtcgacgg acgcgtaacc
     2761 gcggttcagt ccgtcatgca agttcgtgga gccgagcggc tcgatccggc ggatcaactg
     2821 ctctagccgg gagagcgggt cccgccccac cacgaagttc tgtaccagat cgcaggcgtt
     2881 ggtatcgaac agcgtgacgt gcacgatgtc cccttgcttc agctctttga gcgaacgcaa
     2941 aaggccgcgc ttgaggtact ccatgcgacc ctcggcttgc atggaaccgg atcggtcgac
     3001 cacatacgcc aggtttacgt tgcggcgtgt ctccgtagtt gccgggcggc cgcgcaccga
     3061 gagcgccagg gtcgcgagat tcggattttc ggggcttggc tcgatatggg ggagcaccga
     3121 aaagtcgtga tctcgctcca ccggtcgggt atcgaacgaa aagtagttca gcagttcgtg
     3181 cgggcgcacg tgctcttgag gaatgggcgc gtagtggtcg atggcccaga gcacgcgttg
     3241 cgccgacgat agactcatgg tgtcgtcgtt cgagaggtag atccccgcac cccactccgg
     3301 ctctctttgc cgggccggcg gtggcactgc cacctccagg tcgcctttgc gacggtcgtc
     3361 gaattccgat gcggtctggc gcggctctgg ggcgcgctct cgcttggctt cgggcgcttt
     3421 cttggaggcg gccgagtcgg ctcgcccatc ccccggcgca gccgcggcgg gtgcaggcgg
     3481 aggagccagc gccggcggcg cgggcgctgc ggcggcgggt ggaggagccg cggcgtatgc
     3541 gtccttggat gcggtgccag gagcggcgcc ttccgcgacg ggcgagctcg gtaaggcgcg
     3601 cgaaggtttt ggtttagcgg gctgcgcgga gccgccactt accaaagctc cgccggcgat
     3661 acctgccccc gtgcctgcgc ctcctcggta acgggttgga taggggggcg attgatagac
     3721 caccgggggc tggggaaagc actgcgccga accgtccacg aatttgggca tcgaggcgag
     3781 cggcggcaac gctgccactg cagccggata atgcggctcg tgcgcccgcg cggtgaagtg
     3841 gtgagccgca cgtgtttgag tgcggtcggt cgcgatcaag ctcgattcac gcgccccggc
     3901 gtccgttggt gccctgccgc cactggcggc tggttgcgtc ttcgatccca cgcagctgac
     3961 gaccgcgaca gccgccaaca ccgccgagcc agagccaaac ccaaaccaat ttcgcatgca
     4021 cctcttacgg ctgagcgcgc gaattcgagc tatcgaattt cagaaaatgt cggacgatgt
     4081 ggcaacgcct gcgtcgaact tacagccctg cgcaaaatcg gcggtctcca acccactttg
     4141 gctcgattgg actgcgagcc gagcaacggc ccggagcgct cgcggtgcgg cccgcgcatg
     4201 gtcctgcaag taaacagtcc agcggactag tcgtccagca aattactagc cgggaagtac
     4261 cgtgcggcgt tgtacatcga gccctggtct cgagcgctcc gaatacgatt caggatcaag
     4321 aacgcatgca agctcacgac atggacgaag tgatcgccct catgaacggt gtcgttgcgg
     4381 gataccgcga tcgacgcagc cctcgcgcgc tgtttgcggc gatgtatcgc cgtgtcacct
     4441 tgcgtgttca gagcgcgatc gctggagggg ctttcgttga cgggccacgc atggacgggc
     4501 tcgcgaccga gttcgcgaat cgctacttcg cggcgctcga ggcgattgag cacgatcggc
     4561 ggctggcgcg ggcctggcaa attgctttcg acgccgaggc ccgctcgcac acgatgatct
     4621 tgcagcatct gctgttgggc atgaatgcgc acatcaattt cgacctcccc attgcggcac
     4681 tcgcggtcac caaagatggc aatttagctg acctcgagtc cgacttctat gcgatcaccg
     4741 gcgttctcgt ggaactcctc gacccggtac aggcggtggt caatcgattt tcccctctgc
     4801 tcgatgtgct ggaccgggtg ggtggtcgca ccgatgagac catcgtcacg ttttcgatca
     4861 gcaacgctcg cgaagaggcg tggcacgaag ccaatcggct cgcgggggag cccccggtgc
     4921 tgcgtgaacg ctccctgctc agtctggatc gccgcgtagc gctcttggcc gagcgcatca
     4981 tccttcccgg agggccgtgt gggctggcta ccgatttaat cgctcgcacc gaggttgccg
     5041 acattccggt catcgtggac gcgctgaccg ctatcccgtg atcgaccgtg gattgcggca
     5101 attctgcgcc cgaacccggg gaaactagcg agcgcggaaa gccccggtcg gagtctcgtg
     5161 cgcttgcgct atcgtatctt ccatggcgca cgagactcga aaagaacagg acagcttcgg
     5221 cccgatcgac gtccccgcgg atcggctctg gggggcgcag acagagcgca gcttgcggtt
     5281 cttcgcgatc tccaacgagc gggttcctcg cgagctcgtc cgcgcgctgg cgctcgtaaa
     5341 aaaggcggct gccgacgtca acgtggacct cggaattctg gagcttcgca ttggtgaagc
     5401 catctcacgc gccgccgagg agatcttggc tggggcccac gacggagaat ttcccctgtc
     5461 gctgtggcag accgggagcg gaacgcagac caacatgaac ttgaacgagg tgatcgccaa
     5521 ccgcgccagc gagctacttg gaggcgtgcg cggcccgggt cgcctcgtgc accccaacga
     5581 tcacgtaaac ctgagtcaat cgtccaacga cgtgatcccc accgccatgg cgatcgcagc
     5641 caatcgggcc gtccgcgatg aagtcattcc caagctcacc gagttgacga attcgctcac
     5701 cgtcaaggcc ggggccctcc agggagtggt caagatcggg cgaacgcatt tgatggatgc
     5761 caccccactc acgctgggcc aagagctcag cggatgggtc gcgcagctcc ggcacggttt
     5821 gctgcacctc gatagcacac tggaccatct ctcggagctg gcgttgggcg gaacagcggt
     5881 ggggacgggt ctcaactctc cgccgcactt ctccgaaaga gttgccgcgc gtctcgcgga
     5941 actcaccggg tatccgctga tcacggcgcc gaacaagttc gaggcgctgg ccgcacacga
     6001 cgcgctcgtc tttgctcacg gggcgttgaa gacgttggcg gcatccttgt tcaagatcgc
     6061 caatgacgtg cggtggctcg cgtcggggcc tcgatcggga attggggaga tcgttattcc
     6121 cgagaatgaa cccggcagct ccatcatgcc gggcaaagtc aacccgaccc aggcggaagc
     6181 gctcacgatg gcctgcgtac gcgtgttcgg caacgacacc gtagtcaact ttgcgggggc
     6241 gagcggcaac ttcgaactga atgtctacaa gccggtaatc atcgacgcct tcttgcagag
     6301 cgcgcgccta ctcgcggatg gtgcggacag ctttcgcaaa cactgcgtgg aggggctggc
     6361 tcccaaccgc gcccggattg ctcaccatct cgagcagtcc ttgatgctgg tcaccgcact
     6421 ctcacccc
//
LOCUS       DASTKU010000517         4957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1438403, whole genome shotgun sequence.
ACCESSION   DASTKU010000517 DASTKU010000000
VERSION     DASTKU010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438403"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VFQ61_17880"
     CDS             complement(<1..906)
                     /locus_tag="VFQ61_17880"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9956386.1"
                     /translation="MATPASTLIQRFVLGLFLVACGGSPEVTQSSTDPGLGGSATTGT
                     GGRSGGGQGTIVAGGRPAEGGQTARGGASAGPQCGDGSLDAGEECDDGNARDGDGCSS
                     RCQPEPGFQCPTPGASCEECGNGIKESTEECDDGNRTSDDGCSDACQAEPGYQCFEAG
                     KPCERCGDGVADSNEACDDGNTTAGDGCSADCATIETGFHCHVEGVSCERCGDGVVDA
                     HEACDDGNNTSGDGCLFNCSAIEEGFDCATPGEACGQCGNGSRELDESCDDGNVEDGD
                     GCAKDCTAVEVGWQCPVPGAGCELCG"
     gene            1584..2555
                     /locus_tag="VFQ61_17885"
     CDS             1584..2555
                     /locus_tag="VFQ61_17885"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9956387.1"
                     /translation="MTEGSPMTRSVTALASIVALACSTQLFACSTAPGLEEPASETAS
                     KSEAILNGSPVDGNKYAAVGALVYYLPEVGVLDVFCSGTLVAPRAIVTARHCTPDIDL
                     ALSSGLVPAIAFGADVFNPTQVVPITDYVAAPAAPGKEKGLLMDGGRDVAVAHLSTAP
                     TGIKPIKLGEYKDSMLGSKFQIVGFGRYNFEGFYGQKFSGVATARATQGFWYRLLFNN
                     DFDAFRSWYFSDSSMAQPSEAEAKEWWKSFKLEKNYELLAGGLPGEAVACYGDSGGPL
                     LRGSHGCDMAIYGVSFAVEGTFSTLCGLGGGYLVFNRKMLDFIHDAI"
     gene            complement(2642..3091)
                     /locus_tag="VFQ61_17890"
     CDS             complement(2642..3091)
                     /locus_tag="VFQ61_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956388.1"
                     /translation="MSVLTANTTLRARSGAYSISVSSVDPSAPVNFQIGGQPYDSNRT
                     NDSARLSAGETPSGGAPALTIIMHPSDDQPTQWGQNPIKLVVQAVTSDQGEYTGNSAV
                     LYGYFLGENANGLNFYLDQSQSQANFQFGKAGVNTTIDQSMPAIAVG"
     gene            complement(3447..4232)
                     /locus_tag="VFQ61_17895"
     CDS             complement(3447..4232)
                     /locus_tag="VFQ61_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956389.1"
                     /translation="MVADQMSVSNRTGLGVAASQFLDSMPLAYGRAFSTEEIEVHAQI
                     VARRQRQLAHVEACPGSPAARPVCVVADDQPGLLSLVTDALLLHGLNVASARAYCRRR
                     AAGEREAVDFFWLQPATSGEVEDLDPLVLEEFAATLRELLAENAVQAVSPTRFPVGIA
                     PSPPLSRPPPTRVFFDVDALRREELLLVAQGRDFPGLLFAIASALHGQGVRIEASEIR
                     TEAGWARDHFHLSLSSSAKVNSELLFDIQAAVLSAIAAARPGA"
BASE COUNT          905 a         1588 c         1471 g          993 t
ORIGIN      
        1 tccgcagagc tcgcagcccg cgcccggcac gggacactgc cagcctacct cgacggcggt
       61 gcaatccttg gcgcaaccat cgccgtcttc gacgtttccg tcatcgcatg attcatccag
      121 ctcgcggctt ccgttgccgc actgaccgca ggcctcaccg ggagtggcac aatcgaaccc
      181 ttcctcgatc gcactacagt tgaagagaca gccgtctccg ctggtgttgt tcccatcgtc
      241 acacgcctcg tgcgcatcga cgacaccatc accgcagcgt tcgcagctga caccctcgac
      301 gtgacaatga aagcccgtct cgatcgtggc gcaatccgcc gagcaaccat cgcccgcagt
      361 cgtattaccg tcgtcgcagg cttcgttgct gtcggcgacg ccatctccgc aacgttcgca
      421 gggctttccc gcctcgaaac actgatagcc gggttccgcc tggcaagcat cgctgcagcc
      481 gtcgtcagag gtgcggtttc cgtcgtcgca ctcttctgta ctctctttga tgccattccc
      541 gcactcttca caactcgctc cgggggtcgg gcactgaaag ccgggctcgg gctggcagcg
      601 cgagctgcag ccgtcgccgt cacgggcgtt cccgtcatca cactcctcac ctgcatcgag
      661 gcttccgtct ccgcactgtg gccctgcact cgcgcccccg cgcgcggtct gacctccttc
      721 ggcggggcga cctcctgcca cgatcgtacc ttggccaccc cccgagcggc ctccagtgcc
      781 agtggttgca ctgcccccca gtccaggatc ggtgctggac tgagttacct ccggactgcc
      841 cccacaggcc acgagaaaca gtccgagaac gaagcgctgg atcaacgtgc ttgccggggt
      901 tgccaaaggt tcctcgacca gacgagagcg gatgaatccg cgtccttatt tagtggcaag
      961 ccgagatcct cactctgatc atccaggaaa tggggctgac ttcaggcgca cctccgggtg
     1021 ccgacggcgc ttctggcgct gtatccaaca cccatcccac atcggtctcc ttgcgaagac
     1081 atcaactact ccccaccgtc ggcaatccgg agtgttccgc acatttgtcc acagaatcga
     1141 gatccgaata aacacaggac ttgacgttcg tggtttgcac gactcgttag ttgaactcgc
     1201 gtggattcgg tgtgtctaac cgcgctgctc agggcgcgcg agccgccaaa gctcagccat
     1261 gagcgcggcg aggcgaatag ttccaaaaaa gaagcctcga gaagccatgc gcgccgcccg
     1321 cgtctcgccc gacgcaagcc gcatggcgcg agtggcccgc ccaaacgaga gaactatcga
     1381 ggcaagacaa ccagcgaagc cgcccgagca tgcgggccca gtgcggctga gcgcgcaatc
     1441 tctgctcgag acgtctttgc caataggcat gttactcatg aattacgcca tgactggcac
     1501 acagtttctt gtcgcaatcc gcaagaagtt gctgcaactt gagagtgttc ggtgcacact
     1561 ccaaacactc tcaggagtaa ctaatgaccg aaggctcgcc tatgactcgt agtgtgaccg
     1621 ctctcgcatc aatcgtagct ctcgcttgct cgactcagct tttcgcctgc tcgacggccc
     1681 ccggcctcga agagccggcg tccgaaacgg cttcgaagag cgaggctatc ctcaatggat
     1741 cgccggtgga cggcaataag tatgcggcgg tcggggcgct ggtttactat ctccccgagg
     1801 tcggggtgct cgacgtattt tgtagcggca ccctggtcgc tccgcgggcg atcgttacgg
     1861 cgcgtcactg taccccggac atcgatcttg cgctgtccag cggcttggtt ccggcgatcg
     1921 cgtttggcgc tgacgtgttc aatccgactc aagtggtccc catcacggat tacgtggctg
     1981 cgcccgccgc gcccggtaag gagaaagggc tgctgatgga cggcgggcgt gatgttgcgg
     2041 tagcgcatct gtctacggcg cccacgggca tcaagccgat taagctcggt gaatacaagg
     2101 actcgatgct tgggtcgaag ttccaaatcg taggcttcgg tcgctataat ttcgagggct
     2161 tttacggtca gaaattctcc ggtgttgcga cggcccgcgc tacgcagggg ttctggtacc
     2221 gcttgctgtt caacaacgat ttcgacgcat tcaggtcctg gtacttctcg gactcttcga
     2281 tggcccagcc gagcgaggcg gaggccaagg agtggtggaa gagcttcaaa ttggagaaga
     2341 actacgaact gctcgccggc ggcttgccgg gtgaggcggt ggcttgctac ggtgactcgg
     2401 gtggcccgct gctacgcggc agtcacgggt gcgacatggc gatctacggc gtgagtttcg
     2461 ccgtcgaagg cacgttctct acactttgcg gcttgggcgg cggctatctg gtcttcaacc
     2521 gcaagatgct ggacttcatc cacgacgcga tttgatcacg agggctcgat gctaccgatc
     2581 gcggatcgcc cctctgcagt cgaagggcga tccggctccc ttgcgaactc ggaaaagcta
     2641 gtcatcccac ggcaatcgct ggcatggatt ggtcgatcgt ggtgttgacg ccggccttac
     2701 caaattggaa gtttgcctgg ctttgcgatt gatccaagta gaagttcaga ccattcgcgt
     2761 tctcgccaag aaagtatcca tatagaacag cgctatttcc cgtgtactca ccctgatcgg
     2821 aggtcaccgc ttgcaccacc aacttgatgg gattctgtcc ccattgcgtc ggttggtcat
     2881 cggacggatg catgatgatc gtgagcgcgg gtgccccacc ggacggcgtc tcgcccgcgc
     2941 tgaggcgggc tgagtcgttg gttcggttgg aatcgtaggg ctgcccccca atttgaaagt
     3001 tcaccggcgc gctggggtcc acggacgaaa cggatatgga gtaagcgccg ctacgcgcgc
     3061 gaagtgtcgt gttagccgtc aatacagaca tgtgatcctc cctcggaatc gcatcgacaa
     3121 cacagccgtg tgtgaggagc gttgctcctc gcgcctagag atacacccct acacagagca
     3181 ggcgcaacgt ccgtattcgc tgtcgaaaag cgtcgagtcg tcgctcacgc ctggcgccca
     3241 gacatccgcc gtcgctctct cggctctttt ccagctacga gattcgtcac ccctcgcgcc
     3301 agaatcggaa catcgcgaca ttaagatcat cggcgatctt gccggtcgcc gaccatcgtc
     3361 ctcgcgcgtg tcgacgacga tcggactcac gcccacgatt ccaagacgta gggctgctct
     3421 ttggcatgcc cggtccgatt gagccgtcag gcacccggcc gggccgccgc gattgcggag
     3481 agtacggccg cttgaatatc gaagagtagt tcggaattca ctttggccga ggaagaaagc
     3541 gagaggtgaa agtgatctcg cgcccatccc gcctcggtgc ggatctccga tgcttcgatt
     3601 cgaaccccct ggccatgcag cgcgctcgcg atcgcgaaga gtagccccgg aaagtctcgc
     3661 ccttgcgcaa ccagcagtag ttcttctcgg cgtagggcat cgacatcgaa gaacacgcgg
     3721 gtgggcggtg gccgtgatag cggcgggctc ggcgcaatgc ccaccggaaa ccgcgtcggg
     3781 ctcacggctt gcaccgcgtt ttcagcgagc agctcgcgca gagtggccgc aaactcctcg
     3841 agcacgagag gatcgaggtc ctccacttcg cccgaagtcg ccggctgcag ccagaagaag
     3901 tccaccgcct cgcgctcgcc ggctgccctt cgccggcaat acgcgcgggc gctcgctacg
     3961 ttcagcccgt gcaggagcag ggcatctgtc acgagcgaga gcaagcctgg ctggtcatcc
     4021 gctaccacgc agaccggtcg agccgcgggc gagcccggac acgcctccac gtgagcgagc
     4081 tgccgctgtc ggcgcgcgac gatctgcgca tgtacctcga tttcttccgt ggagaatgcg
     4141 cgcccgtacg cgagaggcat ggagtccagg aactgggacg ccgctactcc caacccggtg
     4201 cggttcgaga cgctcatctg atcggccacc atcgcttacc ttgcaggctg ctcgcggccg
     4261 cgttgcgcgc agatatccga ttaatttccg gacgggcaac gccccggctc cgagcctcga
     4321 gccggctcgc tttgcacaac acggatttca cgctgatcat ctcgtgcatc gccggctgca
     4381 cgactcgtga aaccccgtga gcttcggaaa gggctcgttc ggcacccgaa taaacgcatc
     4441 ttgtcgcaga aattacgcca tttactcgcg ccgccctgca cccctcgccg ctgactccaa
     4501 gtgcgcacct gtgactctcg gggatgatca tgccaagtgt tcgggccgcg cgatttgcgg
     4561 ttttcccaac ggactcgcgt cgatgccaat tttccatcgg caacggcgct ggtcgcctgc
     4621 ggccaagctc gcggccgccc tggctcgagc tgacacgcgg aagatgctcc tcggagggag
     4681 tgcgctcggt gtcggatgac cgcggctcga acactgcggc ggctagccct tgaggcacgc
     4741 gccctgcaat gcgcggtgcg gaacgtcgat tatcgacgat tggtatttca caaccccgga
     4801 gcgttatgcg ccgtacattt ggtaatgcag atttgacccg atctgggttc gcgtcgattg
     4861 gctgggtcgg ttgctggatt ctggccgcgg ctggcttgtc tgcttgcgga agtggtgagg
     4921 tggcgtcttc gtcgaactcg gacgaggttg actccac
//
LOCUS       DASTKU010000518         8229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_990417, whole genome shotgun sequence.
ACCESSION   DASTKU010000518 DASTKU010000000
VERSION     DASTKU010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990417"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="VFQ61_17900"
     CDS             complement(<1..1248)
                     /locus_tag="VFQ61_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956390.1"
                     /translation="MPCKQHPTGSDTCVECLIDALVLFHWVDQEKGKVFPAMLMLSKR
                     LAKVKEAEDYASNLGVLEVLVAEANRAALKQDSSVKKLLSLEVPGGSLAVPFDSTEFT
                     SRHRHGYRQYVGRTGSWLGMTEIQALARRFQVTFPVYFTSNQSFFDLGLTVSRDCTIH
                     SHCLVFTGNHWEVGEVEDRNGRVLARRIATNGLGDCSLEAFLTLLAVAYPITAANLRP
                     RLWRSVDAFRALRRTHCTGPTYYDAVPAQSALPVVTELRTLLKGGLTEQEVTDGLAAV
                     PGLRLMAASEESSSTKLLPVTLGKGKWAGYAAHVSQFFRLTKALQCEATADQSLVVIT
                     NIPQVDTASVLKKSGAAVSAALGLTSAPQTQRLYRAVTLPKAGLVRLVHLGVMVACIE
                     RTDGKTTHVVCTIANFAAGRREEV"
     gene            1829..4315
                     /locus_tag="VFQ61_17905"
     CDS             1829..4315
                     /locus_tag="VFQ61_17905"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HET9956391.1"
                     /translation="MSPNRFGQAFGFSGVLIGAAVTVLQACSGDSRVAPPSSLPGSGQ
                     PGVSGAPSVTMGGPPSSGGAAGQPSSSGGLASVGGAGIAGSSWGGSTGQARGGAGGSA
                     TTTDPTPLEPDTCGQGLAATFTTTCAACHTAQGVAHPRYPDLYQFKGTLQDFRGRVRE
                     GSQAGMPAFSADMVSDAELQSIFDYFTRNARSSSPAPAGGVIPLFESADALNPPVVFE
                     RPDGVLVTRGAGRVRGRHEGPLDSNEPFMEFVADYFKSRTYGWIVEDFTRMGQSKIRV
                     TYLPISMPTTGTNFRAWKDYGNGDVFSANMGMTSGAPFPSLLLGNSDLAVSYAEKIAP
                     YAQVQQQETTRNSRSGEAIRAGDLFEFEFGIFIEAGAIEPTGSRTSYYTDTFRYRVGQ
                     GGITPDNADRYTEGKGVLGPVEAAQQGGGTTNVWAYFRPETQFGQMALNIQHENVQHF
                     VEGRRLFHTDFQTGKHSESGNPDFTEQRAKAGPLGTATSCETCHKNNGPGQTLEGALG
                     STSSMAIKLYGESAAGRQLQLTDGSATASAGEKKTVTLADGTSAVLSRPKIAVLMGDG
                     RVPAFSARIARKVIGLGLLESVDENTILVRADRTDCNGDGISGRPNLVHDPRTNELRI
                     GRFGWKAEKVSLEHQNAEALHDDMGVGTSIFPDSGKTELSNEELDRLVTYMRLVSVPG
                     QRSRDDTQVKAGEQLFRTVGCAHCHVTDLVTGVNHPFAELRGQSIKPYTDLLLHDMGP
                     DLADDSKRPASDDASAPPAASEWRTPPLWGTGMLAAINGHTGLLHDGRAANILEAILW
                     HGGEGEAARERVKKLSASDREALIRFVMSL"
     gene            complement(4336..7041)
                     /locus_tag="VFQ61_17910"
     CDS             complement(4336..7041)
                     /locus_tag="VFQ61_17910"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET9956392.1"
                     /translation="MSTKFISCIAAMLALSVSGSVWATGRAEYRAAVQVPIYMGGESS
                     AFRGVARSRLPGALNVSEVPADVGWVRDIDPAFRTDAQPLGAARVLEWSGRGSASYAF
                     VSQPGPLSEVSTPAGESFLIFNLKQLGTSAGNLRITLETGSCRLQYALTERELPQTGF
                     WQTYRIPLEAFQAQGACKKVKALGSLSVVFEKAPRQAGRVGLAALRWENYPKGWSFSE
                     SLTEDLLQGLSVEDKIGQMAQLVHRAGVQNTPVLPADVTRLRLGSLFGDSGAPPHDAN
                     GELLADTPANWVSLLNSYQRAALATEQAIPILFGTDAVHGVSLVPGATIFPHNIGLGA
                     AGNPELVEAIARATAEEAAAIGFRSTFAPTLAVARDERWGRTYESFAETPELVSRLGV
                     AAIRGYQGTRTAKQRTPLSHPLSVLATAKHFGGDGAGEFVDPASWCSTQPCPNGYSTD
                     RQDNALSREQLIRLHLSPYVPAIAAGVGMVMSSDSRFHGDVVTGNHDLLTNLLKNDWG
                     FQGVVITDYGSWSLLDPQNPERSALLSLNAGNDLFMLPQEVGYPALAQAVVNGLATGE
                     LSSSRVDDAVRRILRQKFELGLFDLPFVQSAAAPLVGSAEHRALAREAVQESLVLLQN
                     RKDRFGGPPVLPLRKKAKMYVAGKAADDIGIQSGGWTDVWQGVVGNDLPGTSILEAIT
                     EQAQGQVMHSPDASADTTGYDVGVVVVGEYPYAEFCGDVLGKTFFCEFSRANPAGLVR
                     AIPEIPLPFGLPPPQSLQLGTSSFTYFSPFAGPEVPIPVTVDPVSDLEVVTRVCSKMP
                     CVVVLVSGRPMFIEPALEQSDAFVAAWLPGSEGGGVADVLFGRNGSNFTGKLRHTWPR
                     TPRSAANPSQVSSQPEHDYVPQNVYVSGMGDLNHVLFPVGYGLRY"
     gene            7854..>8229
                     /locus_tag="VFQ61_17915"
     CDS             7854..>8229
                     /locus_tag="VFQ61_17915"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HET9956393.1"
                     /translation="MLPADPDAALARFREALAHVVQLRKVSLHELLELRLVVETAALV
                     RAAQLPVGQHLESARVALETMSEPDTTQEEFYDADVSFHAALVAASGNEALRLVMLAV
                     KDSIRLYLESTMQGRSFAKVRQQ"
BASE COUNT         1618 a         2645 c         2521 g         1445 t
ORIGIN      
        1 tacttcctcg cgccggcccg ccgcaaagtt cgcaatggtg cacaccacgt gagtggtctt
       61 gccgtccgtg cgttcgatac acgccaccat cacccccaaa tggaccaaac gcacgaggcc
      121 agccttgggg agagtgacag cgcggtacag ccgttgggtt tgaggcgccg aggtgagccc
      181 cagcgcggcg ctcacggcgg cgccggactt cttcagcacc gaggccgtat ccacctgcgg
      241 aatgttcgta atcaccacca ggctctggtc tgctgttgcc tcgcactgca gcgctttggt
      301 cagacgaaag aactggctca cgtgcgccgc gtagcccgcc cacttgcctt tacctagcgt
      361 gacgggcaac agcttcgtgc tcgagctctc ttcggaggcc gccatcagtc gcagcccggg
      421 caccgcagcc agcccgtctg tgacttcttg ttcggtgagc ccacccttga gcagagtgcg
      481 tagttcggtg acgacgggca acgcggactg cgcgggcacc gcgtcgtagt aggttggccc
      541 cgtacaatgc gtacgccgca gcgcgcggaa tgcgtccacc gaacgccaaa gccgggggcg
      601 caaattggcc gccgtaattg ggtaggccac cgcgagcaag gtcaggaatg cctccaatga
      661 gcagtcaccc aggccgttgg tggcaatgcg cctggcgagc acccgcccat tccgatcctc
      721 gacttcgccg acttcccaat gattgccggt gaagaccagg cagtgcgaat gaatcgtgca
      781 atcgcgactt accgtcaggc ccagatcgaa gaaactctgg ttcgaagtga agtagaccgg
      841 gaaagtgacc tggaagcgcc gagccagcgc ttgaatctcc gtcattccta gccaagaacc
      901 cgtgcgaccc acgtactgtc gataaccgtg ccggtgccgg ctcgtaaact ccgtagaatc
      961 gaagggcacc gccaagctac cgcccggaac ctccaagctg agcagcttct tgaccgaact
     1021 gtcctgcttg agcgccgcgc ggttggcttc ggcaaccagc acttccagca ccccgaggtt
     1081 cgaggcgtag tcttcggctt ctttgacctt cgccaggcgc ttactcagca tcagcattgc
     1141 cgggaacacc ttgcccttct cttggtcgac ccaatgaaac agcactagcg catcgatcaa
     1201 acattcgacg caagtgtccg aaccggtcgg gtgctgcttg caaggcacga ttcttcctcc
     1261 ctggagcttg agctttcgct tcgcgcgcgt ttttgcgtga ccaacacgtt agcggctagc
     1321 ccgtcaagtg cgcaagaaac gcgctaagcg ttgcgcgcgc ggcgaacggc acctcggccg
     1381 gcgacggagg ccccccacgc gggcagcccg cgtggggggg gccctgccgc gcgcagcgca
     1441 gcgagcacgg cgggacgtca acacgccgtg atccgtggag ggcaactcca gcgcccgaac
     1501 cgcgtgccgt gggccaatcg gtgatccttc agggcggctg agactcggat actgcccgca
     1561 actatctgga tgtgcatcat tcgcaactcg gcacaccatc gcgattcgaa ttcaccaact
     1621 cccaaacgcg accagatgtg aattgtgagg ccgccgcgac ttcagcgacg tgatggccct
     1681 ggaccgtctg cagacggtcc ggtggcagcg cacccgggcc ttcgaccacc cgggggagta
     1741 gctgcctttg cctacattca cgccgctttt tgggcgagtt cgtcgattcg gcaacgattc
     1801 gcggggagag gctccacttt agggcagcat gtcacccaac cggttcgggc aggctttcgg
     1861 cttctcagga gtattgatcg gagcggccgt cacggttctg caggcatgct cgggagactc
     1921 gagagttgct ccgccaagta gcctgccggg ctccgggcaa ccgggagtgt cgggcgctcc
     1981 tagcgtaacg atgggcggcc caccgagttc gggcggtgcg gcgggacaac cgagctcgtc
     2041 gggtggcttg gcgagcgtcg gtggggcggg tatcgctggc tcgagttggg gcggctctac
     2101 tgggcaagcg cggggcggag cgggcggttc ggcgaccacc accgatccga cacccctcga
     2161 gcccgacacc tgcggccagg ggctcgccgc cacgtttacg acgacgtgtg ctgcttgcca
     2221 taccgcacaa ggggtcgccc atcctcgcta tccggatctc tatcaattca aagggaccct
     2281 gcaggacttc cgaggacggg tccgcgaggg cagccaagca ggaatgcccg cgttctccgc
     2341 cgatatggtg tccgacgccg agctgcaatc catcttcgac tacttcacgc gaaacgcgcg
     2401 ttcgagctct ccagcgccag cgggcggagt cataccattg ttcgaatcag cggacgcgct
     2461 gaatcctccc gtggtgttcg aacgtcctga tggagtgctc gtgacccgcg gtgcagggcg
     2521 cgtccgcggc cggcacgaag gcccactcga cagcaatgaa ccgttcatgg agttcgtcgc
     2581 cgattacttc aagtcgcgaa cgtatggttg gatcgtcgaa gatttcactc ggatggggca
     2641 gagcaagatc cgcgtcacct accttcccat ctcgatgccg actacaggta cgaactttcg
     2701 ggcttggaaa gactacggca atggcgatgt cttcagcgcg aacatgggca tgacctccgg
     2761 cgccccattc ccgtcgttgc ttttgggcaa ctcggatctc gcggtctcgt acgccgagaa
     2821 gatcgcgccc tacgctcagg tgcagcagca ggagaccacg cgcaacagcc gttcgggaga
     2881 agccattcga gcaggcgatt tgttcgagtt cgaattcggg atcttcattg aggcgggcgc
     2941 gatcgaaccg acgggatcgc gcacgagcta ttacacggat acgttccgat atcgcgtagg
     3001 gcagggcggt attaccccgg acaacgcgga tcggtacacg gagggcaagg gcgtgctggg
     3061 ccccgtggag gctgcccaac aaggtggtgg gacaaccaat gtttgggcat atttcaggcc
     3121 cgagacacag ttcggtcaga tggcgctgaa catccagcac gagaatgtgc agcacttcgt
     3181 cgaagggcgc cggctcttcc acacagactt ccagaccggc aaacactccg aatccggcaa
     3241 cccggacttt accgagcaac gagccaaggc cggcccgctc gggaccgcca cgagctgcga
     3301 gacctgccac aagaacaacg gtcccgggca gacgctcgag ggcgccttgg gttcgacgag
     3361 cagtatggcg atcaaactat acggtgagag tgcggcgggc cggcagctgc aactcacgga
     3421 cggctccgcc actgcatcag cgggtgagaa gaagacggtc acgctcgcgg acggaaccag
     3481 cgcagtgctc agcaggccca agatagcagt tctgatgggc gatgggcgag tgccggcgtt
     3541 ttcggccaga attgcgagaa aagtgattgg cttggggctc ctcgagtcgg tcgatgaaaa
     3601 tacgatattg gtgcgcgccg atcggaccga ctgcaacggc gatgggatct ccgggcgacc
     3661 caacctcgtt cacgacccgc gcacgaacga gcttcgtatt gggcgcttcg ggtggaaagc
     3721 ggagaaggtc agcctcgagc accaaaacgc ggaggcgctc cacgacgata tgggcgtcgg
     3781 gacgagcatc ttccctgact cgggcaagac cgagctcagc aacgaagagt tggatcggct
     3841 cgtcacgtac atgcgcctgg tctcggtgcc tgggcagcgc tctcgcgacg acacgcaggt
     3901 caaggcgggc gagcagctct tccggaccgt cggctgcgct cactgccacg tcacggatct
     3961 cgtgactggc gtcaatcacc ccttcgcaga gctacgcggt cagagcatca agccctatac
     4021 ggatctgctc ctgcatgaca tggggccgga cttggccgac gacagcaagc gcccagcctc
     4081 cgacgacgcc tctgctccgc ccgccgcgag cgagtggcga acacctccgc tctggggaac
     4141 aggcatgttg gccgccatca acggtcacac gggcctgctg cacgacgggc gagccgccaa
     4201 tattctagag gccatcctct ggcacggtgg cgaaggcgaa gccgctcgcg agcgggtgaa
     4261 gaagctttcc gcgagcgacc gcgaggcgct gattcggttc gtgatgtcac tttagacggg
     4321 ggggcgccac ctagctcaat acctcaaccc gtaccccacc gggaacagca cgtgattcag
     4381 gtctcccatg ccactcacgt agacgttctg cggaacataa tcatgttccg gttgactcga
     4441 tacctggctc gggttggccg cacttcgggg ggtgcgcggc caggtatggc gcagcttgcc
     4501 ggtgaaattg ctgccgttgc gcccgaatag tacatcagcc acgccgccgc cttcgctgcc
     4561 cggcaaccac gccgcgacga atgcgtccga ttgctcgagc gcaggctcga tgaacatcgg
     4621 tcggccactg acgagcacga cgacacaggg catcttcgaa cacacgcgcg tgacgacctc
     4681 caagtcagat accgggtcaa cagtgaccgg aatcggcacc tccggtccgg caaagggcga
     4741 aaagtacgtg aagctcgagg tgccgagttg cagcgattgg ggcggaggca acccgaacgg
     4801 cagcgggatc tctggaatcg cgcgcactaa accagcggga ttggcgcgac tgaattcgca
     4861 gaagaacgtc ttgcccagta cgtccccgca gaactcagca taggggtact cgccaaccac
     4921 gaccacaccc acgtcgtaac ccgtcgtgtc cgcgctggcg tctgggctgt gcatcacttg
     4981 gccctgcgct tgctcggtga tggcctccag gatgctggta cccggcaagt cgttgcccac
     5041 aacaccttgc caaacatcgg tccagccgcc ggattgaatc ccgatgtcgt cggcagcttt
     5101 acccgccaca tacatcttcg ccttcttgcg aagtgggagc acgggcgggc cgccaaagcg
     5161 atctttgcga ttctgcaaca gaactagact ttcctgaacc gcctccctgg cgagcgcgcg
     5221 atgctctgcc gatccgacga gcggggccgc cgcgctctgg acgaacggca gatcgaacaa
     5281 cccgagttcg aatttctgcc tgaggatgcg tcgcaccgca tcgtccacgc gcgaactcga
     5341 gagctccccg gtggcgagtc cgttcaccac cgcttgtgcc agtgcgggat acccgacctc
     5401 ttgaggcaac atgaagagat cgtttccggc gttcaaagag agcagggcag atcgctccgg
     5461 gttctgcggg tcgagtaagg accaggagcc gtagtccgtg atcacgacac cctgaaagcc
     5521 ccaatcgttc ttaagtaagt tggtgagtaa gtcgtggttc cccgtcacca cgtcgccatg
     5581 gaagcgcgag tcgctcgaca tcaccatgcc cacgcccgcg gcgatggccg gcacataggg
     5641 ggacaaatgc agtcggatca actgttcccg actgagggcg ttgtcctgtc gatcggtcga
     5701 gtagccgttg gggcacggct gcgtgctgca ccagctcgcc ggatccacga actcgcccgc
     5761 accgtctcca ccgaagtgtt tggccgtggc caacaccgac aaggggtgag agagcggagt
     5821 ccgctgcttg gccgtgcgcg tgccttgata gccgcgaatt gcggcgacac ccaggcgcga
     5881 aacgagctct ggagtttccg caaagctctc gtacgtgcgc ccccagcgct cgtcccgcgc
     5941 caccgccagc gtgggggcaa acgtcgagcg gaatccgatg gcggcggctt cttccgccgt
     6001 tgcccgcgca attgcttcga ccaactcagg attgcccgcc gcgcccaggc caatgttgtg
     6061 cgggaagatc gtggcccccg gcaccagcga tacgccatgc acggcgtcgg ttccgaacag
     6121 gatcgggatc gcctgttcgg tggccaacgc ggcccgctga tatgaattca acagcgacac
     6181 ccaattggca ggcgtgtccg cgagcagctc accattcgcg tcgtgtggcg gtgcgcccga
     6241 gtcaccaaac aaggagccga gccgcaggcg tgtgacgtcg gccggcaaca ccggcgtatt
     6301 ctggacccct gcacgatgca cgagttgcgc catctgcccg atcttgtctt caacactcag
     6361 accctgtagc aagtcttcgg tcaggctctc cgagaaagac cagcccttcg gatagttttc
     6421 ccagcgcaga gctgcaagcc ccactctgcc tgcctggcgc ggcgccttct cgaacacgac
     6481 actcaggctg ccgagcgcct tcaccttctt gcaggcaccc tgcgcttgga aggcctccaa
     6541 cggaatacgg tatgtctgcc agaaaccagt ctggggcagc tctctctccg tgagcgcata
     6601 ctggagccga cacgaaccag tttcgagcgt aatccgcaga ttccccgcag acgtgccgag
     6661 ttgcttcaag ttgaatatca agaagctttc acccgcgggt gtcgatacct ccgagagcgg
     6721 acctggctgg cttacgaacg catatgacgc gctcccccga ccactccact ccaatacacg
     6781 cgcggcaccg agtggctgag catccgtccg gaacgccgga tcgatatctc gcacccaacc
     6841 cacgtcggcg gggacctcgc tcacgttcaa ggcaccgggc aaacgcgagc gcgcgacacc
     6901 gcggaaggca ctcgactcac cacccatgta aatcggcacc tgcacggccg cacggtattc
     6961 ggcgcgccct gtcgcccaaa cagacccgct cacgctcagg gcgagcatcg ccgcgataca
     7021 cgatatgaac ttagtactca tgtgaattct cccaaaccaa agccggcgca acgcgcgagt
     7081 catgcaactt cgacgctgtc ctgactcagc ccgactccgt ttgcgccggc cttttgcgac
     7141 ggcctctgca aggcgtgagc caagacgggg aaccgcccgt tgcccggcat tcagagccgc
     7201 aaagcccgcc tgcggcgctc gctcattccc cggtgctgct tcgcaaacct tcccaatctc
     7261 gggatcccaa tttcccaaac gtgggaagct ctatggtcgt gatggcgggc aagcagaaga
     7321 acgggcgcga ctactcgaca cctccaccca gcagcgcagc cacctctgcg catctcagcg
     7381 cgagcccacc gcgcaagccc gtccaagccg aacgcgcgcc ccactgcaac caagaaacag
     7441 gaccaccgca aacggccgtc ggctggccac gcttcgagct gacgaaggga cgaagaccgt
     7501 gtcgaagaag tgaaagcggc cgccaggagc gctccgcctt gtcgaagaca acgggggacg
     7561 ctggtcctcc ttgacgccgg gggatggtcc gttggtatgt tggtcggacc aacggacaac
     7621 ggtaaatgag taagacgcga ccttttgaac cgattgctcg cgttacggcc tatgagcaag
     7681 tggcggagcg aattcgcact gcgattttgg accggtctct ttcgactgag cagcgtctgc
     7741 cggccgaacg tgagcttgcg caacagtttg gcgtcagccg aacgactgta cgtgaggccc
     7801 taaggcattt acaggcccag ggcctgctct cttccaaagg aaggacgtcg ccgatgctcc
     7861 cagctgatcc agacgctgcg ttagctcgat ttcgcgaagc actggcgcac gtggtgcagt
     7921 tgcgaaaggt ctcacttcac gagttgttgg agttgcgatt ggtggtggaa acggccgcgc
     7981 tagtgcgcgc ggcgcagctg cccgtggggc agcatttgga atcggcacga gttgcgctgg
     8041 agaccatgtc ggagcctgac acaacccagg aggagtttta cgacgcggac gtctccttcc
     8101 acgcggctct cgttgctgca tccggcaacg aggcgcttcg gctggtgatg ctcgccgtca
     8161 aggacagcat tcgtttgtat ctcgaatcta cgatgcaggg gcgatccttc gcgaaggttc
     8221 ggcaacagg
//
LOCUS       DASTKU010000519         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256 k141_99581,
            whole genome shotgun sequence.
ACCESSION   DASTKU010000519 DASTKU010000000
VERSION     DASTKU010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99581"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFQ61_17920"
     CDS             <1..377
                     /locus_tag="VFQ61_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956394.1"
                     /translation="ASPPRGESLVLELSEVAFDWLISESRNPAVQRLDPYRDTAFSGQ
                     SLKDLLDAVEHALELKTNGARDRVIATSKLPKDPAVRDRILSDLVGRALDTDQVTRSL
                     RELCSFLELAAECAAIVHVDSD"
     gene            682..>2946
                     /locus_tag="VFQ61_17925"
     CDS             682..>2946
                     /locus_tag="VFQ61_17925"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
                     /protein_id="HET9956395.1"
                     /translation="MSALAWFVACLTTLAGTARADRPAPLSAQTLKAPSGPSSLKGLG
                     ESFSANPGTGTGSYSVPITLPPGSVMPSIALRYTGGAGKGEVGVGFTLSPFQIYRMTD
                     KGSPKFDESDRFAVSAPEYNDELVLANASLRIYRMKNEGPDVLFQRDPTNDRWTIIQA
                     NGYKSILGETAAARAQAAGKTTRWFIERGLDVHGHEIKYRYSKDQGKVYWSGVDYQLE
                     ATTPYRNRVEFGYESRPDPVRDYRYGDLEFIASRLKRIEVYQGTRLLRRYTLGYLADS
                     LFSLLATVELEGESGAKMPTLSFGYLGPSLQSGRFTKIEGFSSLAGLDTGRLTLEDVN
                     ADGLPDLLNGTASAYTYAENVDGARFASVVPLGSAGSPDRNLDAAGVVLADVDGDGYR
                     DVWYPVGDGRFRYFPGGRVDRGKFLGFGAAQVMNGNGVVSDVTRPEVRLSDLNSDGRT
                     DLLFQRASLGDVWIENQAASGFVQRAIPTLPAGVLLTNGAVEFTDFNGDSVLDLVLRE
                     FDAGKHDLRVWYGFGSGRFASTEQVIQAPVAGAKEIFLSDVNRDGQVDLVRVSGSWVA
                     YYLNDGRGSFSVRHGDFQGMPETSRTKKILFADMNGNGSTDVVWLTVDGGLSYLDLMG
                     EPHVGLLARIDNGMGLVTDISYRSSTDYAIEAKLSASPWRTPLPHPVSVVSEVSTRDS
                     MELLGVDAEESRTSYDYREGYYDGREREFRGFARVTVTEWGDQFEDSLVRELSMHVGR
                     ALDSGSDEEVLKGKV"
BASE COUNT          548 a          868 c          952 g          578 t
ORIGIN      
        1 tagctagtcc tccgcggggc gaatcgttgg ttttggagtt atccgaagtg gccttcgatt
       61 ggctcatctc agaaagccga aacccggctg ttcagcgcct ggatccgtac cgggacacgg
      121 cctttagcgg ccaaagcctc aaagacctgc tcgatgccgt cgagcacgcg ctggaactca
      181 agaccaacgg cgcgcgcgac cgcgtgatcg cgacctcgaa attgcccaaa gatcccgccg
      241 tccgagatcg gatattgagc gacctggtcg ggagggccct ggataccgac caggtgacaa
      301 ggagtctgcg cgagctttgc tcgttcctag agctcgccgc ggaatgcgcc gccatcgtgc
      361 acgtggacag cgattgacgc aaagataccc cttcgaggcg attggccgct actgggatag
      421 agctcgcacc aactgagtcg gccagagatc ggtcaatcgc acgccggact gcgaacgctc
      481 gagtcatcga gttgtatatc aatcgatgac tgtatacgaa tcgctgacac gttgagcgcg
      541 ccacgtgcga gttcgcaaag ccttgccgcg cttgcgggcg agtgcgtcgt gctgatacct
      601 tgaaggggtt atcgtatcga gcgggagact cgggtcatgt ggatgcggca gttgaaggtc
      661 cattcccgcg ggagggcgct cgtgagcgcg ctcgcgtggt tcgttgcctg tttgacgacc
      721 cttgcaggca ccgcgcgcgc cgatcggccg gcccccctca gtgcgcaaac cttgaaagcg
      781 ccgagtggcc ccagctcctt gaagggcctg ggcgagtcct tctctgccaa cccggggacg
      841 ggcacgggct cgtattctgt acccatcacg ttgccgccgg gttcggtcat gccgtcgatc
      901 gcgctccgct acacgggagg agccggcaaa ggtgaggtgg gcgtcggctt cacgctctct
      961 ccctttcaaa tctaccgtat gacggacaag ggctcgccga agttcgatga gtctgaccga
     1021 tttgccgtca gcgcacccga atacaatgac gagctcgtac tcgcgaacgc gtcgcttcga
     1081 atctaccgga tgaagaacga agggccagac gtgctcttcc aacgggaccc cacgaacgat
     1141 cgttggacca tcatccaggc gaacggctac aaatcgatac tgggggagac ggccgccgcc
     1201 cgtgcgcaag cggcgggtaa gactacacgc tggttcatcg agcgtgggct ggatgtacat
     1261 ggtcatgaga tcaaatatcg atattccaag gatcagggca aagtttattg gagcggcgtt
     1321 gattaccagc tcgaggcgac cacgccatac cgaaatcgtg tcgagttcgg ctacgagtcg
     1381 cggcctgacc ccgttcgtga ctatcgctac ggtgatctgg agtttatcgc gtcgcggctg
     1441 aagaggatcg aggtctacca gggcacgcgc cttcttcgcc gctacacgct cggatacctc
     1501 gcggactccc tcttcagcct gctcgccacc gtcgaactcg agggtgagag cggcgcgaag
     1561 atgccgacgc tgagcttcgg gtatctcggg ccatcgttgc agagcggtcg cttcaccaag
     1621 atcgagggct tctcgtctct cgccggactc gacacgggcc gcttgacgct ggaggacgtg
     1681 aatgccgatg gccttccgga tctcctgaac gggaccgcgt ctgcctacac gtacgccgag
     1741 aacgtggacg gggcgcgctt tgcgagcgtg gtgccgctcg gatcggcggg cagtccggat
     1801 cgcaacttgg atgctgcagg cgtcgtactc gcggacgtcg acggcgacgg ctatcgggat
     1861 gtttggtacc ccgttggcga tgggcgtttc cgctatttcc ccggcggtcg tgtcgatcgc
     1921 ggcaagttcc tcggattcgg cgctgcgcag gtcatgaacg gcaatggcgt cgtgagcgac
     1981 gtcacccgcc ccgaagtccg cttatccgat ctgaactccg atgggcgcac cgatctgctg
     2041 ttccagcggg cgagtctcgg agacgtctgg atcgaaaacc aggccgcgag cggattcgtg
     2101 cagcgcgcca ttcccacgct gcccgccggg gtgctcctca cgaatggtgc cgtcgagttc
     2161 acggacttca atggcgactc ggtgcttgac ctggtgttgc gcgagttcga cgctggcaag
     2221 cacgatctga gggtctggta tgggttcgga agcgggcggt tcgcttcgac ggaacaggtg
     2281 atccaagcac ccgtcgcggg tgcgaaagaa atctttctct ctgacgtgaa ccgcgacgga
     2341 caggtcgatc tggtgcgcgt gagcgggagc tgggtcgcgt actatttgaa cgatggtcgc
     2401 ggcagctttt cggtgcgtca cggggatttc cagggcatgc ccgagaccag ccgcacgaag
     2461 aagattctgt ttgccgacat gaacggcaac ggctcgaccg acgtcgtatg gctcacggtc
     2521 gacggggggc tcagttacct cgatttgatg ggcgaacccc acgtcgggct gctggcccga
     2581 atcgacaacg gaatggggct cgtcaccgat atcagctatc ggtcctctac ggattacgct
     2641 atcgaggcca agctttcggc aagcccgtgg cgcaccccct tgccacatcc ggtgtcggtc
     2701 gtttccgagg tgagcactcg cgactcgatg gagttgctcg gggtggacgc ggaggaatcg
     2761 cgaactagct acgattaccg cgagggctac tacgatggcc gagagcgtga atttcgcggc
     2821 ttcgctcgag tcacggtgac cgagtgggga gaccaattcg aggactcact ggtgcgcgaa
     2881 ttgtccatgc acgtcggccg cgctctcgat tccggcagcg acgaagaggt gttgaagggc
     2941 aaagtc
//
LOCUS       DASTKU010000520         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_546077, whole genome shotgun sequence.
ACCESSION   DASTKU010000520 DASTKU010000000
VERSION     DASTKU010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546077"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..896
                     /locus_tag="VFQ61_17930"
     CDS             255..896
                     /locus_tag="VFQ61_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956396.1"
                     /translation="MSDSAASNTEDGSLRRLFELCGVAPLGVYTLVHVGSYATVLFGG
                     TGFGAPNFRGAQAILEVLLVWLPWSFHALLGLWFSSFPLPSAGSERRRVALSRISAVL
                     GLGFVIWHVLWLRAPIWNRTRITEDLSEMLIGGLSETWNGVPVAAAAHLVGLGLIACH
                     LGLGLPRFFARRELLSKRSAQRLAWWISLGLFGIGAATVLELATGDFPPHFMH"
     gene            981..1415
                     /locus_tag="VFQ61_17935"
     CDS             981..1415
                     /locus_tag="VFQ61_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956397.1"
                     /translation="MPKCYLVAIAGGSSLDRYSNNVTLFNMVEQLNFPKEQPPPPGAV
                     LPLEIHAYFQLAPEEINQRFEVRFSLLSDTGLETLTDGFAHRSQTVRYRTRTVGLPAP
                     PVAGSYELCVDVRAPGSEAWQRQSVRWPLVITETEPRPAVTH"
     gene            1432..2208
                     /locus_tag="VFQ61_17940"
     CDS             1432..2208
                     /locus_tag="VFQ61_17940"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET9956398.1"
                     /translation="MSQRPADYLQLRGLELDCIVGVRPPERTRPQRIRLDVTLGLGLS
                     LAGRSGRFSHTVDYARVADEITALLRFREYRLIEMATEEMAAMLFAAHPALESLEIRL
                     EKPEALRGRALACAVNIARTRQQFRATRSPLPGGHEDCVLETQEARLSLLTLRPDSSL
                     DALTVIDSLMAISPVPAARPLTDDLRAPPDVVRPLSGVLSPAAARALCWVTQGTVTQG
                     TMLDGERSFAAPSEPLVLERGLKSGPEGATLFMCQLYAAS"
     gene            2309..>3130
                     /locus_tag="VFQ61_17945"
     CDS             2309..>3130
                     /locus_tag="VFQ61_17945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956399.1"
                     /translation="MPSPNSSRFRRSLLVLFLTWGLLLIGGVAHAAGRVEWKSKELKE
                     RSGGSWRVELAIFMPRAPDVAHVPMKFEFQPTAYYERSMVDGDKLIERVVPLENRQAL
                     IESVDVGFLDSGSGKIESRTRFTFNVTRAHGYEAGEYKVTIRDSRNGQVVGQAVSLKF
                     TGENEVIDRRAMVFTGNDKKKKKKDAEEGGDKSDKGEDSAKAKSDSSEGSNDEAKEKT
                     ESKSDSDATDSSAASSSEETSGKADDESQDQEIKEKPGGCGCRVIPAQSSSSGLWL"
BASE COUNT          566 a         1013 c          985 g          566 t
ORIGIN      
        1 aaaccgcctg tttagccacg caacgcatca cacaccacca ggagcgcacg aagtcaagcc
       61 ggcgcaggtg cggtcgaagc tgggtcgacg ggcggcgtag tagcgtgagc cgccctgccc
      121 tagcccacga tccgaacgct agcgcctata atcgagcacc aacaccctgt attagcctct
      181 ccgctccgct gccgaactcc gtagcagccc tcagcagcca gccctcagca gcccagcccg
      241 aaagcccagt gagtatgagt gattcagctg ccagcaacac agaagacggc tcgttgcgtc
      301 gccttttcga gctgtgtggc gttgcgccgc tcggtgttta cacgctggtg cacgtgggca
      361 gctacgctac cgtcttgttc gggggaacgg gcttcggagc gccaaacttc agaggcgcac
      421 aggccatctt ggaggttttg ctcgtctggc tgccttggtc atttcacgcc ctgctcgggc
      481 tgtggttcag cagcttcccg ctcccctccg cgggctccga gcgtcggcgt gtggcgcttt
      541 cccggatcag cgccgtgctc gggctcggct tcgtcatttg gcacgtgctc tggttgcgag
      601 ccccaatctg gaaccgcact cggattaccg aagatctctc cgagatgctg attggaggcc
      661 tatcggaaac ctggaatggc gtcccggtcg ccgcggctgc acatctggtc ggcttgggtt
      721 tgatcgcctg ccacctgggg ctcggattgc cgcgcttctt cgcccggcgc gagctgctat
      781 cgaaacgttc ggcgcagcgc ctcgcctggt ggatttctct aggcctgttc ggaatcgggg
      841 cggccaccgt gctcgagctc gcgaccgggg atttcccacc ccatttcatg cactagccaa
      901 cgcgagccac gcgcgcggac acacgaatct caggcgggcg ttgcgggagc cgccccgctc
      961 tgttacggcg ggagcgctga atgcccaagt gttatctcgt ggccatcgca ggcggctcga
     1021 gcctggatcg atattcgaac aacgtgaccc tgttcaacat ggtggagcag ctcaactttc
     1081 cgaaggagca gccacccccg ccaggtgccg tcctgccgct cgaaatccac gcctatttcc
     1141 agctcgcgcc cgaagagatc aatcaacgct tcgaggtgcg cttcagcctg ctttcggaca
     1201 cgggcttgga gaccctgacg gatggctttg cccatcgctc tcagaccgtg cgttatcgca
     1261 ctcgaaccgt cggattaccc gcgcctcctg tggcaggaag ctacgaactc tgcgtggacg
     1321 ttcgagcccc gggcagcgag gcgtggcaac gccaaagtgt tcgctggccg ctcgtcatta
     1381 ccgaaacgga accccgcccc gccgtcacgc actgaacacg cactggaacc gatgagccaa
     1441 cgtcccgcag attatctcca attgcgcggc ctcgaactcg actgcatcgt aggagtgcgc
     1501 ccgccagaac gtacgcgccc gcagcggatt cgcttggatg tgacgttggg gctcgggctc
     1561 tccttggccg ggcgcagcgg gcgcttttcc cacacggtgg attacgcacg cgtcgccgac
     1621 gagatcacgg ccctcttgcg tttccgggag tatcgcttga tcgagatggc cacggaggag
     1681 atggcggcga tgttgttcgc ggcccaccct gccctcgagt cgctggaaat tcggctggaa
     1741 aagcccgaag ccctgcgcgg ccgcgccttg gcgtgcgcgg tgaacatcgc ccgcacccgg
     1801 caacaatttc gggcgacccg cagcccgctg cccggaggtc acgaagattg tgtgctcgag
     1861 acccaagagg cgcggctctc gctgctgaca ctacggcccg actcttccct cgatgctctg
     1921 acggtcatcg attctctgat ggccatcagt cccgtgcccg cggcgcgtcc cctgacggat
     1981 gaccttcgag cgccaccgga cgtggttcgt cctctgtcgg gtgtcctcag tccggcggcg
     2041 gcccgagcgc tgtgctgggt aacccaaggg acggtaaccc aaggaaccat gctcgatggt
     2101 gagcgcagct tcgcggctcc cagtgagccc ctggtgctgg aacgcggtct caagagcggc
     2161 cctgaaggag cgactctctt catgtgccag ctctacgccg cgagctagca ccggaaaact
     2221 tcagccgtat tgggttccag tcgccaccgg tgaaatccag gctcggtggg gcgcccgccc
     2281 gtgttagcgt gccgcgcgga ggtcaccgtt gccgagccct aattcctctc gatttcgacg
     2341 atcgttattg gtcctgttcc tgacatgggg tctgctgctg attggcggcg tggctcacgc
     2401 ggccggacgc gtggaatgga agtccaagga gctcaaagag cgctcggggg gctcctggcg
     2461 cgtcgagctt gcgatcttca tgccccgagc tccggacgtg gcgcacgtcc cgatgaagtt
     2521 cgagttccag ccgaccgcct attacgagcg cagcatggtg gacggcgaca agctgatcga
     2581 gcgcgtggtg ccgctcgaaa atcgtcaggc cctgatcgag agcgtcgacg tcggcttctt
     2641 ggactcgggc agcggcaaga tcgaatcccg cactcgcttc accttcaacg tcacgcgcgc
     2701 ccatggctac gaggcgggtg aatacaaggt cacgatccgc gatagccgca acggccaggt
     2761 cgtcgggcaa gccgtctcgc tgaagttcac cggtgagaac gaggtgatcg atcgccgcgc
     2821 catggtcttc actggcaacg acaagaaaaa gaagaagaag gacgccgaag agggcgggga
     2881 caagtcggac aagggcgagg actcggcgaa ggccaaatcg gacagctccg aggggtcgaa
     2941 cgacgaggcc aaggagaaga ccgagtccaa gtcggacagc gacgccaccg acagctccgc
     3001 cgcgtcgagc tccgaggaaa cgagcggaaa agccgacgac gagagccaag atcaagagat
     3061 caaggagaag cccggcggtt gtggctgccg tgtgataccc gcgcagagct cgagctccgg
     3121 gctgtggctc
//
LOCUS       DASTKU010000521        16545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1661391, whole genome shotgun sequence.
ACCESSION   DASTKU010000521 DASTKU010000000
VERSION     DASTKU010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16545
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661391"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFQ61_17950"
     CDS             <1..235
                     /locus_tag="VFQ61_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956400.1"
                     /translation="MNSDDLGRLAARARERVLEQHHSFRRAERLEQLLETVRARSSVE
                     SPEDARSVVGYPNRNAAPQRWADAELGLLASEV"
     gene            238..1032
                     /locus_tag="VFQ61_17955"
     CDS             238..1032
                     /locus_tag="VFQ61_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9956401.1"
                     /translation="MWGIIPAAGRGSRIQPLAFSKELLPVGSRSDEGRERPIAVSEYL
                     VDRMLAAGVTKLCFVISPGKSDILEYYGGQLGKADICYVVQARPSGLCDAVFRPLPFI
                     SDDESVCVGLPDTIWFPEDGLAKLPDDPLSFLLFPVENPSLFDAVALDSNNRVTQVEV
                     KAAAPKSHWVWGAFKVRGRTLRELAALWREPGRNDEYWGTLVNAYLERGGAAIGVKAG
                     RAYVDVGTLHGYREATRLLDAVKIVSEIQAPPAERREGVGLPALLR"
     gene            complement(1218..1553)
                     /locus_tag="VFQ61_17960"
     CDS             complement(1218..1553)
                     /locus_tag="VFQ61_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956402.1"
                     /translation="MGAQLSALSKRLYRRSQFWLRALASVNAVPGMSLAAATGMNRHF
                     LGISVFLLVSVTLLAAAVIFHWLWLIYAVLVAAPIMGAVMATAFMRPKGHPPEHHTSV
                     DRYLNDTDR"
     gene            1877..3055
                     /locus_tag="VFQ61_17965"
     CDS             1877..3055
                     /locus_tag="VFQ61_17965"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9956403.1"
                     /translation="MTLVGLAAGYWGIPAAHGTERQSEFTAVPFVGGDSDMGWGGGAL
                     ASWARLSPGLEPYRLRVEAATALTVRRDGEDVELPYTDAYLLFEAPHALPHRLKLRAR
                     LSYTRETRLKYYGLGNAAEPAGEPTLYGRTHPTLDVQAEYRVTEAVRVIWGVNYTQNW
                     LKVAEDSQLGRDMVQGSTEVREILGRAKDHAVVSFSYGLTFDTRDDIVNPQTGVLAAA
                     RVDLAPGGSGALVHRYARLNTSVRGFLTLMPNRLVLAARVVGDLLSDGAPFYELGRYD
                     QTSAIGGIQGVRGVPAQRYIGKLKVFGNLELRSHLFAARLFQKSTRFGLTGFVDTGRV
                     WSDYQSHPELDGSAIGLKLGLGGGLRVSAGKSFVLRFDVGWAKEAEPIAAYLTSGHAF
                     "
     gene            complement(3060..4280)
                     /locus_tag="VFQ61_17970"
     CDS             complement(3060..4280)
                     /locus_tag="VFQ61_17970"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9956404.1"
                     /translation="MASTLRLPSFSSSEPDRSLPDSQKLEIKPAPQDPKVAPAIPPEA
                     LDAVDDAPLSEAPQRRGARQRAATRGTRWMVAVVSVGAVLSLIPLWEPLLLAAFAAIV
                     AEPLYAKLSRKLNGRSRAAGVVTLLLVVAVLSPTVVASLSIYKGAIELVTRLRQSADG
                     SDALKQLVSGQPGIQHIDLSGAGLMKYLREHGGGALTAAQTVFGAATTVVVALFVFAI
                     GFHSFLVDGRRAYAWVLKRSPIPRTHVVRLGNAFTETARGLLVGVGLTAIIQGAVATI
                     GYIALGVPQALVLGLLTTMAALIPSVGTGLVWVPVGAGLFLAGRTQAAVILGCIGIVV
                     SVVDNLLRPWLSRYGKLDLSGFVVLVSMLGGVAMFGGFGILLGPLLVRLATESWQLLH
                     DEQLDAPLARRAAP"
     gene            4582..7413
                     /gene="ligD"
                     /locus_tag="VFQ61_17975"
     CDS             4582..7413
                     /gene="ligD"
                     /locus_tag="VFQ61_17975"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HET9956405.1"
                     /translation="MRALKKYQEMRDFSRTAEPRGPADEKGGAKLRKTQTALTYVIQK
                     HAARRLHYDFRLELDGVLLSWAVPKGPSFDPRVKRLAVETEPHPIEYAQFEGGIPKGE
                     YGGGTVMVWDRGSWTPEGDARAGYKKGHLRFTLEGEKLHGAWHLVRTARPNSKDKNWL
                     LFKSTDDRHGEERDSTPDVVLSAPNSVLSGRSLEQIAKDVESGRTSAPEQPVQQTGLD
                     SSGDSKESNRKPRRAKRSASVENLAASEQLVLAGARKEKLPSFVEPQLATLVSDPPDG
                     EEWVHELKFDGYRVLARIDNGDVRLLTRRGLDWTERMPTFARAVRDLAERRSIRTAIF
                     DGEFVALNARGISDFQELQNSLGTEAESLVYYVFDLLHLNGVDLRSVVLTQRKLLLRE
                     LLAQEIVPPAAGATPGMEPSPLATASPNPERARLGAKPRGKAKDLESDQHGVRAMIRF
                     SEHMAGSGRAFFERACKLGLEGIISKRAVATYHSGRGRDWLKTKCLARQEFVIVGYTE
                     PSGSRTHLGALLLGVRKEDELVYCGRVGTGFNAQSLAELHERLAPLAIAKPLRLGGVP
                     KGAEARGVHWVRPELVAEVAFTGFTQDGALRHPAFQGLRDDKLAGEVGLERPRPTRDL
                     LSNPATKKKSGERKSKKANSAGTNQPEPDAHRIAEGYPLSNADKILYPEQGITKRELL
                     DYYALVAERMLPHVANRPLTLVRCPNGRGKACFFQKHPGPAVPEGLRSVAIREQEGKA
                     PCSVIDDALGLFGLVQLGALEIHTWGSRADDFEHADLLVFDIDPDPALDFSAVIRCAH
                     RLRQLFESAKLESFVKTTGGKGLHVCVPIEPKLEWDQIKAFTGSVAEALSKESPDLYV
                     ATVSKAKRQGKIFIDYLRNARGATFVAPYSTRARENAPVAVPLEWDELTPKFTPSTFT
                     VRTLAKRLKHMARDPFERLISVQQRLPID"
     gene            complement(7541..7975)
                     /locus_tag="VFQ61_17980"
     CDS             complement(7541..7975)
                     /locus_tag="VFQ61_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956406.1"
                     /translation="MPSNKQSSSAGQRGSVGRESERVIRSSAGGGASSRSGAGMSSGG
                     SLPGGSGTATSESDENYALISVIYHALQGADTSVRYIDDARRSGDTELVTFFEDCQRL
                     DRERAARARQLLAERLVEDLDDEDEDEELDDEEDDEDDDEGV"
     gene            complement(8001..9536)
                     /locus_tag="VFQ61_17985"
     CDS             complement(8001..9536)
                     /locus_tag="VFQ61_17985"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET9956407.1"
                     /translation="MTSTSKRIRTGAFSKQWLVMGALITLSGYACSDDDDTTPGPSGG
                     RSATGGSGGRSGGTTGSVQGGRSSGGRSSGGTVAVPEAGEAGGGTLGIGGEQPGAGAG
                     GSAEAGRGGEGGTPESPQSGGSAGVGGVGGEGGVSDGSGGVVSADPDVFRPAQLPMSQ
                     ELVDSLSLPTGFQVNVFASGLKHARMLATHGPHLYVTRPMQNDVLVMTDADQDGVADT
                     PTAAVTGLSLVHGIAFHGDNVYLSTVSAVYRAAVNPNGTFGTPAVIVDDLPGGGQHPY
                     RTLGVGPDDKLYISVGSDCDACAETNPENAALLRTNLDGTSREIYATGLRNMLGFGWH
                     PTTGKLWGVDNGSDWRGDLLPPEELNQVVLGADYGWPYCFGKQQIDPVIQDPPDKTKA
                     AYCASTQPSVLEDDAHGAPTGLVFYSGTSFPEVYRGAAFVALHGSWNRFPPSGYKIAQ
                     VRFDAQGNPVAFSDFVTGFLVQGATAQFGRPTALTVGPDGALYFSDDANGVLYRVQYV
                     NVE"
     gene            complement(10068..11051)
                     /locus_tag="VFQ61_17990"
     CDS             complement(10068..11051)
                     /locus_tag="VFQ61_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956408.1"
                     /translation="MVSLTRMLTFVALCLASRLAYSFNDLFTGRLARREGRVEVAALR
                     GASLAVSMAPLLLWVPSESFGRLGRFGGELALTCALTAASNVLQLEAARFLPFGARAA
                     LIVGGVSGGSLLLGWLLSGEVLAATDVALGLLVMTSAVAASGGAHATNEIDPNPGKGA
                     LLSLGSAALAAIVSWEVQNLARRSNALLVAWAWEGGSGLVLLGPLLLWPGVAQRLCSA
                     RRFMRIALASLPTVVGSGASVLALSYGELGLWGAFAGTQILFTVALGTLLHRERLGAL
                     RAVCFALAAGAIAALALRRVPGSASQQSQVNPSALVYPGYSSVAFPRAPAA"
     gene            11141..11773
                     /locus_tag="VFQ61_17995"
     CDS             11141..11773
                     /locus_tag="VFQ61_17995"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9956409.1"
                     /translation="MQTEQPRERTYLPLVFLPGASGRSAVWQPIAQRLASRRQPVLVD
                     YPTMAEVSSHYSTRGLPGLAELLLPTLPKRFDAAALSMGGALALHLVLKHPERVRRLI
                     LVTTAGGINVQALGALDFRPSFVERRHGERNWFVEDASDFGPRLGEIEQPCLLVFGDR
                     DPIAPLAVGQFLKTGLRQARLEILPGATHDLEQEFPDQLASLIEAHLRGT"
     gene            11876..13111
                     /locus_tag="VFQ61_18000"
     CDS             11876..13111
                     /locus_tag="VFQ61_18000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9956410.1"
                     /translation="MLSPPSPASSELERQDARQRPLLLALGSATFMVSLDARVIAPLL
                     PTIAKDFQISVAQAGWLVSGYMLPYGLFQLVYGPLADRFGKVRVSAWAMLAFSAGTAV
                     CGVFGAFHSILLFRALTGAAAAALIPLTIAYIGDTVPYRRRQSALAMLMASAGAAQAF
                     STSAGGTIAALLSWRTVFPLLGATAAASTAALFVLRGRELRVVAAPGESPPRYIDALR
                     APRMLRLLLLVALEGVLFMGGFPYLSGLLELRFRMTALGIGLVLGSAGVAQLVGARLL
                     PLALRRLGERGLLWVGGGQMSAAYLLCAVARGWGTVVLACCLAGIGFSLAHSTLQTRA
                     TELFPRGRATALSLFALSLLCGGGLGSLLFGYASEELGYTSTFVASGLLFVAFTATSV
                     RALGYGEFAKHGMPQTSIE"
     gene            13340..14239
                     /locus_tag="VFQ61_18005"
     CDS             13340..14239
                     /locus_tag="VFQ61_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956411.1"
                     /translation="MTAPRQILPGSTYFVTRRCTQRMFLLKPSPETNSLILYCLAEAA
                     EETGVILHAVCFMSNHWHGVLSDPFARLPEFLERFHRRIARALNVVLDRRENFWSSEK
                     TTVILLATERDIVDRMAYTLANPTAAGLVSSPADWPGIISRNFEKVLRVEMPEILAGL
                     QEGGPDLVHLSFMRPPAYQSLSNGEFSTLLDSLVIHRVRRAHAEMRRTGQQFLGAKQV
                     LKQSAESKPTTPGKAPLHGLPVAASDPKIREVAVFKLKKFLHAYREAFRSWCRGERHV
                     AFPFGTYALRVHFGIPCAPPAPS"
     gene            14790..16304
                     /locus_tag="VFQ61_18010"
     CDS             14790..16304
                     /locus_tag="VFQ61_18010"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HET9956412.1"
                     /translation="MLKPNICGFDGFKSPEKTGDDGVRGRVTNPEFVRGVIHCLKRRG
                     HERITIAEGCGNSNAHWRRAVAVSGFDQLAREEGIPLVALDDDGFFDVEGTKPGKPLA
                     ISGIEKSHVPTLLMPRLLGEILQDGLFISIPKLKTHRYSVVSLGIKGMQGTVQRSEAS
                     PAYNQKWRMHAELKEYLRGLRAGVDDRAAYVASLEAFTERVLDVLEISLPDAVLLEGT
                     PAMSGDGFQTLRAIPGMIAIGGTHPVWVDRVGAEYLGLWDNAGLAAELAGHRSSPLIE
                     RAAERYGLDLRYPGLRGDGVKMLKEKRPVFFKALAPFTIQDQSPDQTPAPTPSPLGGA
                     PQTSQLGPKQRPEARAFFTSSAPQIDGKADSQWEQATPAVWNTDYAGMPTATTTRARF
                     LWQKHALFALFELSNTSLNVDSSFPVDRERPKLYQEDCVELFLSPNPEYPGHYFEIEL
                     GPRGHWFDLEVDRSRGVERSEWSSGVRLSTRANETNHSALIEAQFTAPELLKAI"
BASE COUNT         3248 a         5172 c         5025 g         3100 t
ORIGIN      
        1 catgaactcc gatgacttgg ggcgactggc ggcgcgcgct cgagagcggg tgctcgagca
       61 gcatcactcg ttccgtcggg cggagcgcct ggagcagctg ttggagacgg tgcgagctcg
      121 ttcgagcgtg gaatcacccg aagacgcgcg gtcggtcgtc gggtacccta atcgaaacgc
      181 ggcccctcag cgctgggccg acgcagagtt gggtctactg gcttcggagg tttgagcatg
      241 tggggtatca ttcccgccgc tggtcgtggc agtcgcattc agccactcgc cttctccaaa
      301 gagctgttac cggtgggaag ccgcagtgac gaggggcgcg agcggccgat tgccgtcagt
      361 gaatacctcg tcgaccggat gctcgctgcg ggtgtcacga agttgtgttt cgtgatctcg
      421 cccgggaaaa gcgacattct cgagtactac gggggtcagc tcggtaaggc agacatctgt
      481 tatgtggttc aagcccgtcc cagcggtcta tgcgacgcgg tgtttcgacc tctgcccttc
      541 atctcagacg acgagtcggt ctgcgttggg ctgccagaca caatctggtt tcccgaggac
      601 ggcctggcaa agctacccga cgaccccttg agtttcttgt tgttcccggt cgagaaccct
      661 tctctattcg acgccgtagc tctcgattcg aacaatcggg tcacccaagt cgaagtcaag
      721 gcagccgcac ccaaaagcca ttgggtttgg ggtgcgttca aggtgcgagg ccgcaccctg
      781 cgcgagctcg cggccctgtg gcgcgaaccc ggacgtaacg atgaatattg gggaacgctc
      841 gtcaatgcct atctcgagcg cggcggcgcg gcgattggcg tcaaggcggg tcgggcttac
      901 gtcgatgtcg gcacattgca tggctaccgt gaggcgactc gcctattgga tgccgtgaag
      961 attgtgtccg aaattcaagc cccccctgca gagcggcgcg aaggagtcgg gcttccggca
     1021 ctactccggt agagcaacgc actcgaagga ccacacgaat ctccgtatgc ttgaccacca
     1081 ggtcggccgc acttgggtcg cgcaggaagc tccgttcacg ctcgtgcctc aacctggatt
     1141 cgcacgagtc cgagttgaag cacagaatgc gagcgtcgac ctggtcgttc ttcaggccat
     1201 ctgtccaggg gttccgctca gcgatcagta tcgttgagat agcgatcgac gctggtgtgg
     1261 tgttcgggcg gatgtccctt cggccgcatg aacgcggtcg ccatgactgc gcccatgatc
     1321 ggggccgcca ccagcaccgc gtagatgagc cacaaccagt gaaagatcac tgccgctgct
     1381 aggagcgtga cgctcacgag cagaaaaaca gagatgccga gaaagtgtcg gttcataccc
     1441 gtcgcagcag caaggctcat gccggggaca gcgttcaccg acgcgagcgc gcggagccag
     1501 aactgggatc gtcgatagag ccgcttcgaa agggcagaca gctgcgcgcc caaactggct
     1561 ggcaaagacg ccttcgattt acggcctccc ccgaactcgc tgttcctttg cgccaaagaa
     1621 tttagacggt tcgcgctcga acccgactcg agcgacggct tccaacatgg cgggctaacg
     1681 tgctgtcgga tgctccactt cgaaagtgta ttgtcgatcc tgccattgcg ccacgctgaa
     1741 gcccgttcag cgcgcacagc attgcctcgc gaggtgcgtt ggcgaggtgg cgcgagactt
     1801 gcgactgtgg gtctgcgtgg ttctgatctg ctcatggcgt cgccggggtt gcagctcgcc
     1861 gctcactcgc tctgtgatga cactggtcgg gttagccgcc ggctattggg ggattccggc
     1921 tgcgcacggt accgaacgac agagcgagtt taccgcggtg cctttcgtgg gaggcgacag
     1981 cgatatggga tggggtggcg gtgcactggc ttcttgggcg cgcctctcgc ctggactcga
     2041 gccctaccgc ctacgcgtcg aggctgccac cgcgctgacc gtgagacgcg acggcgagga
     2101 cgtagagctc ccctacacag acgcttatct gctcttcgaa gctccccacg cgctcccaca
     2161 tcgcttgaag ctccgcgctc gattgtctta tacacgcgag acgagactca agtactacgg
     2221 cttgggcaac gccgctgaac ctgcgggcga accgaccctt tacgggagaa cccacccaac
     2281 cctcgatgtc caagcggaat accgcgtgac ggaggcggtg cgtgtcattt ggggcgtaaa
     2341 ctacacgcag aactggttga aggtggctga ggattctcag ctgggccgcg acatggtcca
     2401 gggctcgacc gaggttcgag aaatcctcgg ccgcgcgaaa gaccacgccg tagtttcctt
     2461 ctcctacggg ctgacgttcg atacgagaga tgacatcgtc aatcctcaga ccggggtgct
     2521 tgcggcggcc cgagtcgacc tcgctcccgg cggctcggga gcgctcgtgc accgatatgc
     2581 gcggctaaac acgagtgttc gtggcttcct gactctgatg cccaatcggc tggttctcgc
     2641 ggcgcgtgtc gtcggcgatc tcctgtcgga cggagccccc ttttacgaac tcggacgcta
     2701 cgaccagact tccgcgatcg ggggaattca gggagtgcgg ggcgtgcccg cccaacgcta
     2761 catcggcaag ctcaaggtat ttggtaacct cgaactccgc agccacttgt tcgccgctcg
     2821 tctgttccag aagagcactc gctttgggct cacggggttc gtggatacgg gccgagtctg
     2881 gagcgactat cagtcgcacc ccgagctgga tggttccgca ataggcctga agttgggact
     2941 ggggggtggc ctgcgcgtct ccgcgggcaa gtcgttcgtg ctgcgcttcg acgtcggttg
     3001 ggcgaaagaa gcggagccga tcgcggccta cctaacctcc ggacacgctt tctgaggctt
     3061 cagggggccg cgcgccgggc aagcggagca tcgagctgct catcgtgtag caattgccaa
     3121 ctctcggtcg ccaggcgcac gagcagcggg ccaagaagga tgccgaagcc gccgaacatg
     3181 gcgactccgc caagcatcga gaccaacacc acgaacccgg aaagatcgag cttcccgtag
     3241 cgagacagcc aaggtcgaag cagattgtcc acgacggaga ccacgatgcc gatgcagccc
     3301 aggatcaccg cggcttgcgt acggcctgcg aggaagagcc ccgcgcccac tggaacccag
     3361 accaggcccg tacccacgga cggaatcaga gccgccatcg tagtgagtag ccccagcacg
     3421 agcgcttggg gcacgcccaa cgcgatatag ccgatcgttg cgaccgcgcc ttgaatgatc
     3481 gcggtcaagc ccacacccac caaaagccca cgcgcagtct ctgtgaaggc atttcccagc
     3541 cgcaccacgt gtgtccgcgg gattgggctg cgcttcagca cccacgcgta cgcccgacgg
     3601 ccgtccacga ggaagctgtg gaaaccgatt gcgaaaacga ataaggcaac gaccacggtg
     3661 gtggctgcgc caaatacagt ttgcgcggca gtcagcgcgc cgccgccgtg ctcgcgcaag
     3721 tacttcatga gccccgctcc actcagatcg atgtgctgaa tgccaggttg acccgacacc
     3781 agctgcttca gcgcgtcaga accatccgcc gactgtcgta gccgcgtgac caactcgatg
     3841 gcacccttgt agatggatag actagccacc accgtggggc tcaataccgc tacgaccagc
     3901 agcagcgtga ccacgcctgc cgctcgtgat cggccattga gcttccggct cagcttcgca
     3961 taaagcggct cggccacgat tgccgcgaaa gcagccagca acaacggttc ccaaagcggg
     4021 atgagggaga ggacggcccc gacgctgacc acagccacca tccaacgtgt gcctcgagtg
     4081 gcggcgcgct gccgcgcacc gcggcgttgg ggagcctcgg aaagtggagc atcatctact
     4141 gcgtccaatg cctcgggcgg aattgccggg gcaaccttgg ggtcctgcgg ggcgggtttg
     4201 atctccagct tctgcgagtc tggcaggctc cgatcgggtt cgctcgatga gaacgacggt
     4261 aagcgaaggg tcgatgccat ggttacctcg atgtctggac caatcggaac tggagagaac
     4321 ttggagcgga gcggcgttta cagagcatgt cgcccgcccc aggatgccgt tcgaattcta
     4381 actcaacagc ttgtcccagc cctggcagca aaaggttcga tgtgggattg aagagagctg
     4441 ccatgctagg taggcaataa actacgaagt acctgcagca aagctcgtgc cggggcttcg
     4501 ttgatgcgtt cgcaggcacg aacgctgcta cgtggcacct ggagcaaagc gatcgagctt
     4561 actccaaggc aaccgttccc catgagagca ctgaagaagt accaggagat gcgggacttc
     4621 tcccgtacgg cggagccccg cggacctgcg gacgagaagg gcggggcgaa attgcggaag
     4681 acgcaaacgg ccctcaccta tgtgatccaa aagcacgctg caaggcgcct gcattacgac
     4741 ttcaggcttg aattggacgg cgtccttttg agctgggctg tgcccaaggg acccagcttc
     4801 gatccgcgcg tgaagcgatt ggccgtggaa actgaacctc atccaatcga gtacgctcag
     4861 ttcgagggcg gcattccaaa gggtgagtac ggcggcggca cggtcatggt atgggaccgg
     4921 ggctcttgga cgccggaggg agatgcgcgt gccggttaca agaagggtca cctccgtttc
     4981 acgttagagg gcgagaagct gcacggcgcg tggcacctcg tccgaaccgc tcgtcccaat
     5041 tccaaagaca agaattggtt actgttcaag tctacggacg atcggcacgg ggaagaacgg
     5101 gactcgactc ccgacgtcgt gctgagcgcg cccaacagcg tgctcagcgg caggagcttg
     5161 gagcaaattg cgaaagacgt agagtcagga cgcacctccg ctcccgagca acctgttcag
     5221 caaaccggcc ttgattcgag tggagactcg aaggagtcga accgcaagcc ccgacgagcg
     5281 aagcgcagcg caagcgtcga gaatcttgcc gctagcgagc aactggtcct ggcgggcgcc
     5341 cggaaggaga agcttccaag cttcgtcgag cctcagcttg caactctcgt cagtgatcct
     5401 ccggatggtg aagagtgggt tcacgagctc aaattcgacg gttaccgagt tctcgctcgg
     5461 atcgacaatg gtgatgtacg gctgctcaca cgccgcggac tagactggac ggagcggatg
     5521 cccacgttcg cccgcgctgt tcgagacttg gcagaacgga ggtcgattcg gaccgcgatc
     5581 ttcgacggag agttcgtggc gctcaacgca cgagggatca gcgactttca ggaacttcag
     5641 aactctctag gaaccgaggc cgagtcgctc gtatattacg tgttcgactt gctgcacctg
     5701 aatggtgtcg acctaagatc cgtagtgctc acacagcgca agttgctgct gcgcgagctg
     5761 ctcgcccagg agatagtgcc tccggcggcg ggggcaacgc cgggcatgga accaagtcct
     5821 ttagcaacgg cgtccccaaa ccccgagcgc gcgcggctcg gggcgaagcc tcgcggtaag
     5881 gccaaagacc tcgaatccga tcagcacggt gtacgcgcca tgattcgctt tagcgagcac
     5941 atggcgggtt cgggccgcgc cttcttcgag cgagcctgca agctcggctt ggagggcatt
     6001 atctccaagc gcgcggtggc aacctatcac tcgggccgcg gccgtgactg gctcaagacg
     6061 aagtgtttgg cccgccaaga attcgtgatc gtcgggtaca cggaaccgag cggctcacgc
     6121 actcacttgg gagcgctgct gctcggcgtg aggaaagaag atgaactggt ctattgcggg
     6181 agggtgggta cgggcttcaa tgcacaatct ttggccgaac tccacgagcg attggcgcca
     6241 ctcgcaattg ccaagccact ccggctgggc ggcgtgccca agggcgcgga ggcacgcggc
     6301 gtccattggg tgcgccctga gctcgtagcg gaggttgcgt ttacgggctt cactcaggac
     6361 ggtgcgctac gccacccagc gtttcaagga ttgcgcgacg ataagctcgc aggcgaagtt
     6421 ggtctcgagc ggccccgccc gactcgagac ctgctttcga atcccgcaac gaagaagaaa
     6481 tctggtgaac gcaaaagcaa gaaggcgaac tcggccggca caaaccagcc cgaaccagac
     6541 gcgcaccgga tcgccgaagg ttatcccctc agcaacgccg ataagattct ctatcccgag
     6601 caaggcatta cgaaacgcga gctgctggat tactacgctc tagtagcgga gcgaatgctg
     6661 ccccacgtcg ccaatcgacc cttgacgctg gtgcgctgcc ccaatggcag gggaaaggcc
     6721 tgcttctttc agaagcatcc cgggccggca gtgcccgaag gattgcgctc ggttgccatt
     6781 cgagagcagg agggcaaggc tccctgcagc gtgattgacg acgctttggg tctattcgga
     6841 ctagtgcagc tgggggccct ggagattcac acttggggct cacgggcaga cgatttcgaa
     6901 cacgcagacc tcctggtctt cgacatcgat cccgatcctg ctttggattt ctcggccgtt
     6961 attcgttgtg cgcatcggct gcgccaactc ttcgagtcag ccaagctcga aagtttcgtg
     7021 aagaccacgg ggggcaaggg cttgcacgta tgcgtgccaa tcgaaccaaa actcgagtgg
     7081 gatcagatca aggccttcac cgggagcgtg gccgaagcgt tgagtaagga gtcccctgac
     7141 ctgtacgtcg cgacggtatc caaggccaag cgacaaggta agatcttcat cgactatctt
     7201 cgcaatgcac gtggcgcgac attcgttgcc ccctactcga cgagagcccg ggaaaacgcg
     7261 ccggtcgcgg ttccgttgga gtgggatgaa ctcactccca agttcacgcc gagcacgttc
     7321 acggttcgaa cactcgccaa acgtctgaag cacatggcgc gagatccgtt cgagcgcttg
     7381 atttccgtgc agcaacggct tccgatcgat taggaccgaa cagtcggcga gcgtcccagg
     7441 aggacgattg tcctgcatgc aggacaatct gccccaggcg gggccgagct gggtgattag
     7501 ctcgagccac cgccgttagc tcgcgattcg agcctttacg ctagacgccc tcgtcgtcgt
     7561 cttcgtcgtc ctcttcgtcg tctagttcct cgtcctcatc ttcgtcatcg aggtcctcga
     7621 cgaggcgctc tgcgagcaat tggcgcgctc gggcggcgcg ctcacgatcg agtcgctgac
     7681 aatcttcgaa gaacgtaacc agctcggtgt cacccgagcg tcgtgcgtcg tcgatgtatc
     7741 gaacgctggt gtccgcgccc tgaagcgcgt gataaatcac gctgatgagc gcgtagtttt
     7801 cgtcggactc cgaagtagcc gtgccgctgc ccccgggcaa gctcccaccg cttgacatcc
     7861 ccgctccact gcgtgatgac gctccacccc ccgccgagga tcggattacg cgctcgctct
     7921 ctcgacccac ggaaccacgc tggcctgcac tgctgctttg tttgttgctg ggcatcgttc
     7981 gtcctcctct aaactcgaga tcattccacg ttcacgtact gaacgcgata gagaactccg
     8041 tttgcatcat cagagaagta cagggcgccg tcgggtccca cggtaagcgc cgtggggcgg
     8101 ccgaattgcg cggttgcacc ctgaaccagg aagcccgtca caaagtccga gaacgcgacg
     8161 gggttacctt gagcgtcgaa ccgcacttgc gcaatcttgt agcctgaagg cgggaaacga
     8221 ttccaggagc cgtggagagc gacgaaggcc gcaccccggt atacctcggg gaagctcgtt
     8281 ccactataga agacgagacc cgtaggagcg ccatgcgcat cgtcctctag gacggacggc
     8341 tgagtgctcg cgcagtaagc ggctttggtc ttgtccggcg gatcttgaat gacgggatcg
     8401 atctgctgct tgccgaagca atatggccac ccgtagtccg ccccgagtac gacctgattc
     8461 aactcctctg gtggtagtaa gtccccacgc caatccgagc cattgtctac gccccaaagc
     8521 ttacccgtgg tcgggtgcca accaaaacca agcatgttgc ggagcccggt tgcatagatc
     8581 tctcgactcg ttccatctag gttcgtacgc agcagggcgg cgttttccgg attcgtctcg
     8641 gcacatgcgt cgcaatcgct gcctacggag atatagagtt tatcgtcggg gcccacgccc
     8701 agtgtgcgat aggggtgttg tccaccaccg ggcaagtcgt ccacgatgac cgcgggggtt
     8761 ccgaacgtgc cgttcgggtt gacggccgct ctatagactg cactaacggt cgacaagtag
     8821 acgttgtctc catggaatgc gatgccgtgg accagcgaca gccccgtcac agctgcagtt
     8881 ggcgtgtctg caacgccgtc ctgatcggcg tccgtcatca ccaggacgtc gttctgcatt
     8941 ggacgagtta cgtagaggtg gggcccgtgt gtggcgagca tgcgggcatg cttcaagccc
     9001 gaggcgaaca cgttgacttg aaagcctgtc ggtaggctca gtgaatcgac gagttcctgc
     9061 gacatgggca attgtgcggg acggaagacg tctggatcgg cgctaacgac gccaccggag
     9121 ccatcggaga cgccaccctc cccgccaacg ccccctacgc cagcgctacc tccggactgg
     9181 ggactttctg gcgttcctcc ttcgccaccc cgccctgctt ccgcgctacc gcccgcgccg
     9241 gcacccggtt gctcgccgcc aatgccgagc gttccgccac cggcttctcc ggcttcggga
     9301 acggcgacgg ttccaccgga gcttcgaccg ccagagctgc ggcccccttg cacgcttccg
     9361 gtggttcccc cgctgcggcc cccagagccg ccggtcgccg atcgtcctcc cgagggaccg
     9421 ggagtggtgt cgtcatcgtc agagcatgca taaccactca gagtgatgag agcgcccatc
     9481 accagccatt gcttggaaaa tgccccggtg cgtatccgtt tactcgtcga agtcatgagt
     9541 atcctctcgc tgctcaatct cgttcacgag ccgtgcggct cttaagggaa cagcgcccag
     9601 cgactagcaa cctacgtgcc cagaccgtca cggctacttc acgccccaat gtgcctcggt
     9661 cgcagccaat tgcagttgga tctcctcgtt cgcgcgcggt cacgcgccaa agcacccgat
     9721 cactcgcgcg atgataattc tgcgaacgcc acagtgagtc tacatcgcga agcaccgctg
     9781 tcgatcaact cgtattcgag tcgagttcgg cagctctcat tcagcgacgg tgagggcctc
     9841 gtagtgagct ctccaagata actgtcagca atagagaaat ccctgacagc ctgaggatgg
     9901 tggcttgcgc atcatgcgca atgcaaaccg cgggcccttg tcgaatatga gtgtcgcgca
     9961 agctggccag aatcaaccat acgcacaagt cgcagccccg catctcattg ctgagcgacc
    10021 cgggatacac gcagttccag ttacgggaac cggaatgcgg ggccaactca cgcggccggc
    10081 gcccgtggaa acgcaactga ggagtatccc gggtagacga gcgcgctcgg attgacttgg
    10141 ctttgttgcg aggcgcttcc cggcacgcgc cttagcgcca gcgccgcgat ggcaccggca
    10201 gccagagcga aacacacggc gcgaagcgcc ccgaggcgct ctcggtgcag caacgtgcca
    10261 agagccaccg tgaacaagat ctgagtccca gcgaaagcgc cccatagtcc aagctcccca
    10321 taggacaacg ccagcacgga cgcgccagaa ccaacgacag tgggcagaga tgcgagcgca
    10381 atcctcatga accgccgagc ggagcacagg cgctgcgcga caccgggcca aagcagcagc
    10441 gggcccagga gcaccaaacc ggagccccct tcccaggccc acgccaccag gagagcgttg
    10501 ctccgtcggg ccagattctg gacctcccag gatacaatcg cggcgagcgc agcggacccg
    10561 agagatagga gcgccccctt tcccgggttc gggtcgatct cattcgtggc gtgtgcgccc
    10621 ccgctggccg ccaccgcact cgtcatcacc aacaggccga gcgccacgtc ggtggccgcc
    10681 agcacctctc ccgaaagaag ccatccgagg agcagacttc ctccgctaac cccaccgacg
    10741 atgagcgctg cgcgtgcccc gaagggcaga aaacgcgccg cctccagctg gagcacgttg
    10801 gaagcggcgg tcaaagcgca ggtcagcgcc agttcgccgc cgaatcgccc gagacgcccg
    10861 aagctttcgc tcggcaccca cagaagcaac ggagccatac tgacggccaa agatgctcca
    10921 cggagagccg cgacttccac ccggccttcg cggcgggcaa gccgtcccgt aaacaaatcg
    10981 ttgaaagaat aagcgagccg agaggccaag caaagagcga cgaaggtcaa catccgcgta
    11041 agtgagacca tcccgggccg accactttcg gcacccgggc agtcgaaaga gcgccggata
    11101 ctcgacccaa ccgcaaagcg aatgcaagga ggcgatcacg atgcagacgg aacaacccag
    11161 agagcgtact tacttgccct tggtgttcct gccgggcgcg agcggccgct ccgccgtttg
    11221 gcagcccatt gcccagcgcc tggcaagccg aaggcaaccg gttctcgtgg actaccccac
    11281 gatggccgag gtctcctctc actactccac tcgcgggctt cccggcctcg ccgagttact
    11341 gcttcccacc ttaccgaaac gattcgatgc agccgccctg tcgatgggcg gcgctcttgc
    11401 ccttcacctg gtactgaagc atccagagcg cgtgcgccgg ctgatcctcg tcaccacggc
    11461 aggaggaatc aacgttcagg cgttgggcgc gctagacttt cgtccctcat ttgtcgagag
    11521 gcgccatggc gagcgcaact ggttcgtaga agatgcgagc gattttgggc ctcgccttgg
    11581 cgaaattgaa caaccatgcc tgctggtttt cggggatcga gatccgatcg cccctcttgc
    11641 ggtcggccaa tttttgaaga ccgggttgag gcaagctcgc ctcgagatcc tacccggcgc
    11701 gacgcatgac ttggagcaag agtttccaga tcagctggcg agcttgattg aggcgcactt
    11761 gcgcggcacc tgacttgaac ccacccgggt tgaatacgaa ccgcatcagg acgacacatt
    11821 cccagtgcat cagttcctcg gcgcgccctg tagaacatgg tatcgagccg cccctatgct
    11881 gtccccgcct tcgccagcat cctccgagct cgagcgacaa gacgcaagac aacgtccgct
    11941 cctgctcgcc ctaggcagcg caaccttcat ggttagcttg gacgcgcgag tcatcgcccc
    12001 cctccttccc acgatcgcga aggatttcca gatcagcgtg gcgcaggcgg gctggctcgt
    12061 ttctggctac atgctgccct acgggctgtt ccaactcgtt tatgggccgc tcgccgatcg
    12121 ctttgggaag gtgcgggtgt ccgcttgggc gatgctcgcg ttcagcgcgg ggaccgcagt
    12181 ctgcggcgtg tttggagcgt ttcacagcat tcttttgttc cgcgcattga ccggcgcggc
    12241 cgccgccgcg ctcatccccc taaccattgc ctatattgga gacaccgtcc cttatcggcg
    12301 acggcaaagc gccttggcga tgttgatggc aagcgctggt gctgcgcagg ccttcagcac
    12361 gagtgccggc ggaactattg ccgctctgct ctcgtggcgc actgttttcc cgttactggg
    12421 agccaccgcc gccgccagca cagccgcgct gttcgtattg cgcggccgcg agctgcgcgt
    12481 cgtcgcggca cctggagaaa gcccccctcg ctacatcgac gcgcttcgcg cgcctcgtat
    12541 gcttcgacta ttactgctag tcgcgctcga gggggtcttg ttcatgggcg gtttccctta
    12601 ccttagtggt ctcttggagc tgcggtttcg gatgacggcg ctcggtatcg ggttggtgct
    12661 cggcagcgcg ggcgtcgctc aactagttgg tgcgcggctt cttccgctcg ccctgcgacg
    12721 tctcggggag cggggcctcc tctgggtggg cggcggacag atgagcgctg catacttgct
    12781 ctgtgcggtg gcaagaggct ggggaacggt cgtgctcgcg tgctgcctgg cggggatcgg
    12841 ctttagcctg gcgcattcga ccctacagac acgcgccact gaactattcc cgcgaggacg
    12901 agccaccgcg ctatcactgt ttgcgctctc gctcttatgc ggcgggggat tgggcagctt
    12961 gctcttcgga tacgcttctg aggagctcgg gtacacctcg acgtttgttg cgtccggcct
    13021 gctcttcgtc gcgttcacag cgacgagcgt gcgcgcgctc gggtacggtg aattcgccaa
    13081 gcacggcatg ccccaaacct cgatcgagtg atcctcagcc cacccgggat actctctgat
    13141 cagcagtggt cccagcgcac ccgggatact ctctgatccc agcccacccg ggatactctc
    13201 agatcagagg gtgggggcga aacccaagga cgccagcccg cgccgaggaa cgcaggcccg
    13261 ccgccatggc catgacggag tgcccccaac agaagaagaa atcaggaaac tcgcgattcg
    13321 tgcgggccga tagaccatca tgacagcgcc gagacaaatc ctgccaggca gcacctactt
    13381 cgttactcgc cggtgcactc aaaggatgtt cctactcaag ccatccccgg aaacgaacag
    13441 tttgatcctg tattgcctgg cggaggcagc agaagaaacg ggagtcatcc tccatgcggt
    13501 gtgcttcatg tccaatcact ggcatggtgt actgagcgat ccgttcgccc gtttacccga
    13561 gtttctcgaa cgatttcatc ggcgaattgc ccgagcactc aacgtagtcc tggaccgacg
    13621 ggagaacttc tggtcttcgg agaaaaccac cgtgattcta ctcgcgactg agcgggatat
    13681 agtagaccga atggcttaca cgctcgcgaa tccgaccgcg gctggtctcg tttcgtcacc
    13741 cgcagactgg ccggggatta tttctcgaaa cttcgaaaag gtgctgcgcg tcgagatgcc
    13801 agagatcctc gccggtctac aagagggcgg tccggaccta gttcacttga gtttcatgcg
    13861 gccccccgcg tatcagagtc tcagcaacgg cgagttctct actctgctgg actcgctcgt
    13921 catacatcgc gtccgcaggg ctcacgcaga aatgagaagg acgggccagc agtttctggg
    13981 tgccaaacag gtcctgaagc agagcgcgga gtcgaaaccc actaccccgg gaaaggctcc
    14041 gctccatgga ttacctgtag ccgccagcga tcccaaaatc cgcgaggtag cggtattcaa
    14101 actcaagaag ttcctgcacg cttaccgcga ggcattccgt tcttggtgcc gtggggagcg
    14161 acacgtcgcg tttcctttcg ggacgtacgc gcttcgcgtg cactttggca ttccatgcgc
    14221 gccgcctgcg cccagctgag ccgttttagc cggagggcag cttcgagcaa ctggacggcc
    14281 caccactccc gaatgcgaac ctcatcgcgc tacctgccgc caccgctctc gtgaaatcgg
    14341 cggcgccgga gcatgatacg cggctagtgt atggtccgta accgaggcct ccaccgttgc
    14401 tcaatcttcg aactctactc aatctagctt gctgcgcttc attgctcatc tgcttcggca
    14461 gctgcaaagc caaatctcaa gaggtcggct cgcatattcc agctagctcg tctccgcgga
    14521 cgtccccggc gagcgcgccc gtctcatcag caactgaatc tcgcgtacca gacacccttt
    14581 cacacgcgag tccggcgtat ctgaccaatc ccggccctgt tcaccccgca ccctcgcgag
    14641 tggacggagg agccttgcgc aagcgccacg tcggccggct gaagtcaggg cactccccgg
    14701 taaccgtgct ggcaggaggc gatgcggtct cccttggcga gcggctgtgt gacgcggtcg
    14761 ttccgcggcg gagcgcaggc atccccgtcg tgctcaagcc gaatatttgc ggttttgacg
    14821 gtttcaaaag cccggaaaag actggagatg atggggtgcg tggtcgcgtc accaatcccg
    14881 agtttgtccg cggtgtgatt cattgtctca aaaggcgcgg gcatgagcga atcactatcg
    14941 ccgagggttg cggcaactcg aacgcccatt ggcgaagagc cgtggcggtg tccggcttcg
    15001 atcaactggc cagagaggag gggattccgt tggttgcgct cgatgatgat ggtttcttcg
    15061 atgtggaggg caccaagccc ggcaaaccgc tcgccattag cgggatcgag aagagtcacg
    15121 ttcccacgct gctcatgcct cgattgctgg gcgaaatcct gcaagacggc ctgttcatca
    15181 gcatcccaaa gctaaagact catcgctatt ccgtggtctc gctcggaatc aaaggtatgc
    15241 agggtacggt tcagcgctca gaagcaagcc ctgcttacaa tcagaagtgg cggatgcacg
    15301 cggagctgaa ggaataccta cgagggctgc gggcgggcgt agatgatcga gccgcgtacg
    15361 tcgcctccct cgaagcattt actgaaaggg tgttggacgt cctcgaaatc tctctacccg
    15421 atgccgtctt actcgagggg acccccgcga tgtccggtga cggcttccag actttgcgcg
    15481 ctatcccggg aatgattgcg atcgggggaa cccaccctgt ttgggtcgac agagtcggcg
    15541 cagagtacct gggactttgg gacaacgcgg gtctcgcggc ggagctcgcc ggacaccgaa
    15601 gttccccatt gatcgagcgc gccgcagaac gttacggcct ggacctgagg tatcccgggt
    15661 tacgggggga cggcgtaaag atgctgaagg aaaaacgtcc ggtgttcttc aaggctctcg
    15721 ccccgttcac gatccaagac caatctcccg atcaaacccc agcaccaaca cctagtcctc
    15781 tgggtggggc acctcagacc tctcaactag ggccgaaaca gcgccctgaa gcacgcgcct
    15841 ttttcacgtc atccgcgccc cagatcgacg ggaaagctga ctctcagtgg gaacaagcaa
    15901 ccccggccgt ctggaacacg gattacgcag ggatgccgac cgcgacaacg acgcgagcgc
    15961 gcttcctgtg gcagaaacac gcgctgttcg cactatttga gctttccaac acgagtctaa
    16021 acgtggactc gtcgtttccg gtcgatcggg agcgacccaa gctctaccag gaagattgcg
    16081 tcgagctgtt tctctcgcca aacccggagt atcccgggca ctacttcgag atcgagctcg
    16141 gaccccgtgg acattggttc gatttggagg tggatcgctc ccgcggcgtc gaacgcagcg
    16201 aatggtcgag cggcgttcga ttgagtactc gcgcgaacga aacgaatcat tcagcgctaa
    16261 tcgaggcgca gttcactgcg cccgagttac taaaagccat ttagtcgggt gcccggttgc
    16321 cctacgcttt gtaccgcatg gagggccggg caccgcgaca gtatctggcg tggagccctc
    16381 cccgaacagc gaagcccaac tttcacgtgc ccgctgcgtt cggcacgctc tacatcgaag
    16441 cgccccactg aggagcggcg acgcgaaccg gtgtacccgg gatactccgg cttggcgtgt
    16501 acccgggata ctccggcttt gggagacctc cgttctttag gtttc
//
LOCUS       DASTKU010000522         4658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_547225, whole genome shotgun sequence.
ACCESSION   DASTKU010000522 DASTKU010000000
VERSION     DASTKU010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547225"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFQ61_18015"
     CDS             complement(<1..698)
                     /locus_tag="VFQ61_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956413.1"
                     /translation="MRTKTTHILGIAAILSAAGLVLACSSNDTPNNATGGAGTTGGAT
                     VTSGGATASGGATTATGGASATGGASATGGTTATGGASTASGGATATGGTASGGASGG
                     AGGASAGAGGATAGAGGASGGAGGGGTSSMGGASGGASGGMSNGGASGGMAGGGAGGS
                     GGDTVSFSKIGALLDSKCGNCHAGYKSSAVAGSFKNTDSKCSNHPFVTPGDSSKSALI
                     ERVEGTCGTKMPIGS"
     gene            1071..2501
                     /locus_tag="VFQ61_18020"
     CDS             1071..2501
                     /locus_tag="VFQ61_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET9956414.1"
                     /translation="MISQPPEVRQGPQLADKVLSKLSRVLGDSKVLTSEESRAAYQHD
                     DSEAEGQLPCAVVLANSEADIQATLAVAQDLDVPVTPRAAGTGRTGGATPVSGGIVLV
                     TLGMNAIREIDTREGLAVVQPGVVLKDLHAAVEAEGWFYPPDPNSLANCALGGNVAEN
                     AGGPRAFKYGVTRDYVLGLTALLIGGQRLQVGRRTIKGVTGYDVTGLLVGSEGTLAVF
                     SEITLALIPKPPCVTTLLALFTDVASAGACVAEMTGRARLVPRCIELLDSGTLEALRR
                     AGNPIDARAGALLLIEVDGSETECDAQAERVGLACEVQGALEVLAATEPSQRERLWAA
                     RREMSYAVRRMSKRKLSEDVVVPRQCIPTLLDRVALSASKHGIRTLTYGHAGDGNLHV
                     NFLWDEPHEAPRVESAIRELFENVVALRGTLSGEHGIGVLKAPYLPLEQSPELIALQR
                     DLKRVFDPKHLLNPGKIFPPAAHRAC"
     gene            2686..4380
                     /locus_tag="VFQ61_18025"
     CDS             2686..4380
                     /locus_tag="VFQ61_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956415.1"
                     /translation="MLLAVVISVVAVASGLGLGLAPAVTRRAIGPLRTLSFTAVLAVV
                     FLHLLPEALRDLGPLGLLVFGLGLALPRWTASIRGEVRVAREDDNASFQREPRASLAS
                     RALSVWPRLGLELGFWGLLIHHVGDGLALGAYSRVSAPDEHVHSDVLLALVLHTVPLV
                     AVVTSSYVRAFGMRSALARGVALAVASLVGVLFAGSVPSIWVDLVRPWIAASVSGLLL
                     HSLAHDLGSDLPTTHLRRFVDFAMAVLGGVLGYWGERLSSRVEHVESAVAKSMDTVAA
                     SVQTPIEFALRLNLERLAAPLLVGAVFGAWLPIIAAIMSGGETGADTERANQGEPAPD
                     AHAPHSIKSGAWSPEAFLLNLAHFGFVFAIVRDAASLLGLALRVPAKDKVRRVASEIA
                     AADVPHSSPAAKSSVIERVALRFDAQVAWALLGVLAAAVVNVALPDASLLPYVLTGTL
                     GMAIFAASVPLHAAAAPPLALALMGKGLAWPAGFLLVWLAPLSAELAWRRRLPLAALG
                     ALGLEVAARFWNLPSPVMPTGFGTPAAALFLVLVAYRAYRVGLRGLLATVAPHSPH"
     gene            complement(4431..>4658)
                     /locus_tag="VFQ61_18030"
     CDS             complement(4431..>4658)
                     /locus_tag="VFQ61_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956416.1"
                     /translation="VPVTIEGSREGLLEALLVGDDASEAEVRAALVRYEQEHGWMREI
                     PSEFTASVGTLSPLLTGETSVLALPFAVRVR"
BASE COUNT          687 a         1477 c         1580 g          914 t
ORIGIN      
        1 gagccaatcg gcatcttggt tccgcaggta ccctcgacac gttcgatcag cgcgctcttg
       61 ctcgaatcac ccggagttac gaacgggtga ttcgaacact tgctgtccgt gttcttgaag
      121 ctcccagcga ctgcggagct cttgtatccg gcgtggcagt tgccgcactt ggagtcgagc
      181 aacgctccaa tctttgagaa gctgacggtg tcgcctccgc tgccgcccgc gccgccgccc
      241 gccatcccgc cgctcgctcc accgttggac atgccgcccg aagctccgcc gctcgctccg
      301 cccatggagg aggtgcctcc tccgccggcg ccgccgctgg ctccacctgc gccggccgtt
      361 gctccgcctg cacccgcgct cgctccaccc gcaccaccgc tggctccgcc actggcagtg
      421 ccgccggttg cggtcgcgcc gccggaagcg gtgctcgctc cacccgtcgc agtggtaccg
      481 ccggtggcac tcgctccgcc ggtcgcgctg gcgccgccgg tcgcggtggt ggcgcctccc
      541 gaagcagtcg ccccaccgga tgttacagtg gcgccgccgg tcgtacctgc cccgcccgtg
      601 gcgttgttgg gggtatcgtt gctggagcag gcgagaacca ggcccgcggc gctgagaatt
      661 gccgcaattc caaggatgtg agtcgtcttc gttcgcatga gtcgaggttc cttcgagcga
      721 gccggcctca gccgaggtgc ccgcagccgc aaatagaagg cccgcccacc caaccccgtg
      781 caactgatta ctaatcgacg gaaagttgcg atatgtcagt aatgtccatg cgttcgctca
      841 tgtttattaa ccgcgggggt taagtcgtgc ccacgctgtg ggcgaaccta acgaaaacga
      901 agcagaaatc gccgcggtgc gacatcactc ggaagcctgt cccaagggcc gaatccgctc
      961 tctggactgt cccgaaacat tgacttgctc gaggagccga aattgaggaa caccgcgggg
     1021 gccgcgggga aggggcgagc gggtggactc ggcggagcta gactccgcgc gtgatctcgc
     1081 agccccctga agtgcgccaa ggtccacagc ttgcggacaa agttctctcc aaattgagcc
     1141 gcgttttggg ggattccaag gtgctgacca gtgaggagag ccgcgcggct taccagcacg
     1201 acgactcgga agcggaggga cagttgccct gcgccgtcgt gctcgcgaac tccgaagccg
     1261 acattcaggc taccctcgcg gttgctcaag atttggacgt gcccgtcact cctcgcgccg
     1321 caggaacggg aagaacgggc ggagcgacgc ccgtatccgg cggaatcgtg ctggtcaccc
     1381 tgggcatgaa cgcgattcgt gagatcgaca cgcgagaagg actcgcggtg gtgcagccgg
     1441 gggtcgtgct caaagatctc catgccgcgg tcgaagcgga aggctggttc tatccgcccg
     1501 atccgaactc gctcgcgaat tgcgcgctgg gcggcaacgt ggctgaaaat gccggcggcc
     1561 cgcgggcttt caagtacggg gtgactcggg attacgtgtt ggggctcact gcgttgttga
     1621 tcggcggcca acggctccaa gtcgggcgac gcacgatcaa aggcgtcacg ggctacgacg
     1681 tcacgggctt gctcgtcggt agtgaaggta cgttggcggt gttcagcgaa atcaccttgg
     1741 cattgattcc gaagcctccg tgcgtgacga cgctgctggc tctattcacc gacgtggctt
     1801 cggcgggggc gtgtgtggcg gagatgacgg gtcgagcgcg cttggtgccg cgttgtatcg
     1861 agctcttgga ctccgggaca ctggaggcct tacgtcgcgc aggcaatccc atcgatgcgc
     1921 gtgctggagc gctgctgctg atcgaggtgg acggctctga aaccgagtgt gacgcccagg
     1981 ccgagcgggt tgggctcgcg tgcgaagttc agggagccct cgaagttctt gccgccaccg
     2041 aaccttcgca acgcgagcgg ctctgggccg cgcgccgcga gatgagctac gcggttcggc
     2101 gtatgtcgaa gcgaaaactc agcgaagacg tggtcgtgcc gcgccagtgc atcccgacgc
     2161 tgctcgatcg ggtggcgctc tccgcgtcga agcacggcat tcgcacgctg acctacgggc
     2221 acgcgggaga cggtaacctg cacgtgaatt ttttgtggga cgaaccgcac gaggcgcccc
     2281 gcgtagaaag cgccattcgc gaactgttcg agaatgtcgt cgcgctgcgc ggaactttga
     2341 gcggtgagca tgggattggc gtgttgaagg cgccgtactt gccgctggag cagtcccccg
     2401 agctgatcgc cttgcagcgc gacctcaagc gcgtcttcga ccccaagcac ttgctcaatc
     2461 cgggaaagat ctttcccccg gccgctcatc gggcctgttg agctggcggg cggcgattgg
     2521 gcgaaggagg gctcggacgc ttcacggtcg ggcgcgcgga ttcggggccc cggagtgctt
     2581 ttcgcgccta ccgaacgggc ccgacgcccc aggcggagct gacaagatcg gcagtgcccc
     2641 cgaaatagcg gctcgagcgc caaagatccg ctagaacccg gcgccttgtt actcgccgtc
     2701 gttatctccg tcgtggccgt ggccagtggg ctcggtctgg ggctcgcgcc cgcggtgaca
     2761 cgtcgtgcga ttggacccct gcgcacgctc tcattcacgg ccgtgctcgc ggtggttttt
     2821 cttcacctct tgcccgaggc tttgcgcgac ttgggcccgc ttggcctgtt ggtgttcggg
     2881 ctcgggctgg cattgccccg ttggacggcg tcgatacgcg gtgaagttcg cgttgcccga
     2941 gaagacgaca acgcttcgtt ccagcgagaa ccgcgagcga gcctcgcgtc gcgcgcgctg
     3001 agcgtctggc cgcggttggg cttggagctc ggcttttggg gcttgttgat ccatcacgtg
     3061 ggagacgggt tggccctcgg ggcgtactcg cgcgtgagcg cgcccgacga gcatgtccac
     3121 tccgatgtct tactggcgtt ggtgcttcac acggtgccgc tcgttgcggt cgtcacgtcg
     3181 agttacgtcc gcgctttcgg catgcggtcg gctctggcgc gcggggttgc gctggccgtg
     3241 gccagcttgg tcggcgttct cttcgcgggc agcgtcccca gtatctgggt ggacttggtg
     3301 cgcccctgga ttgccgcgtc ggtttcgggt ttgctgctgc actcgctcgc ccatgatttg
     3361 ggcagcgatt tgcccacgac acaccttcga cggttcgtcg attttgcgat ggcggtcttg
     3421 gggggagtgc tcggatactg gggtgagcgc ttgagctccc gcgtggagca cgtggagagc
     3481 gccgtggcca agtcgatgga tacggtcgcg gccagcgttc agacgccaat cgagttcgct
     3541 ctgcggctca atttggagcg tttggcggca cctctgttgg tgggtgctgt ctttggggcg
     3601 tggctcccca tcatcgcggc gatcatgtcc ggtggtgaga cgggcgcaga caccgagcgt
     3661 gcgaaccagg gagagcccgc gcccgacgcg cacgcacctc actcgatcaa gagtggtgcc
     3721 tggagcccgg aagccttctt gttgaatctc gcccacttcg ggttcgtgtt tgcgatcgtg
     3781 cgcgatgcag cctcgttgct gggcctcgca ttgcgcgtgc cggcgaaaga caaggtgcgg
     3841 cgagtggcgt ccgagatcgc ggccgcggac gtgccgcatt cttcgcccgc cgcaaagtcg
     3901 tcggtgatcg agcgcgtcgc gctgcgcttc gacgctcaag ttgcgtgggc tctgctgggt
     3961 gtgctcgcgg ccgcggtagt aaacgttgct ttgccggatg cttcgctctt gccctacgta
     4021 ctcaccggga cgctcggtat ggcgatcttt gcggcttctg tgccgctcca cgccgcggcg
     4081 gcacccccgc tcgccttggc gttgatgggc aaagggctcg cttggccggc gggattcttg
     4141 ctcgtgtggt tggcgcccct gagcgctgag ctcgcttggc gacgacgcct gccattggcg
     4201 gcgctcggag cgttggggct cgaagttgcg gcacggttct ggaacttgcc gagcccagtg
     4261 atgccgacgg ggttcggcac gcctgcggcc gctttgttct tggtgctagt ggcttatcgc
     4321 gcgtaccgcg tgggtttgcg tgggctgctc gcgacggttg ctccgcactc tccgcattga
     4381 aatcgtgatg actaggagcg cgagcccgcg gtcctcacgc tccggcgcgc tcaacgcacg
     4441 cgtactgcga aaggcaaggc caggaccgag gtctcccccg tgagtagcgg gctgagcgtg
     4501 cccacgctgg ccgtgaactc gctcggaatc tcgcgcatcc agccgtgctc ttgttcgtaa
     4561 cgcacgaggg ccgctcgaac ctcggcttcg ctcgcgtcgt caccgaccag gagcgcttcc
     4621 agcaagcctt cgcgcgagcc ctcgatcgtg accggcac
//
LOCUS       DASTKU010000523         3926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_101484, whole genome shotgun sequence.
ACCESSION   DASTKU010000523 DASTKU010000000
VERSION     DASTKU010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101484"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VFQ61_18035"
     CDS             <1..1204
                     /locus_tag="VFQ61_18035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9956417.1"
                     /translation="FADGRYESRAPLFGGSAAKQLRDIDHSVVPSPTDPGHAYLVTRV
                     PVREHAHLLGIIEIAETLDMRDDFVRTSAWNTAIATLALVLVSGMVALAVGVVMVGRP
                     LRLLANKAKRVGIGDLTGPLHLKQRDEIGELAREVNAMCEKLAEANERSALETRARLK
                     ALEQLRHADRIITVGRLAAGIAHELGTPLNVVAGRVKMLRRSRAEPPMLDEYLTIIAE
                     QVERMALIIRQLLDFARRREPRIHTHDLVAIAGTIARLLEPIARRKSVQVTLTTTNPV
                     LARIDAARIEQVISNLVVNAIHACAAGGHVEISCGLQNDEGELRQRIAYVKVRDDGHG
                     MDEDTVARIFEPFFTTKDVGQGTGLGLSVAHGIVEEHGGRISVESTPGEGSVFTVYLT
                     ADERAEAS"
     gene            complement(1298..3628)
                     /locus_tag="VFQ61_18040"
     CDS             complement(1298..3628)
                     /locus_tag="VFQ61_18040"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET9956418.1"
                     /translation="MTQDEVGQSQLRRSTLHRVQIWYCVKVRRALRFLLVLLVGLGVL
                     TMLAYWGITGTTREWFQKDLRLRTELAVSAAGQSLAANWTDPHKLRESLTNIARDERV
                     IGAAACSPTAEVLTATEHFPSEFSCSVVLNGSMSQVASHNRTWWMTSESSLGHLQVSA
                     TSLESEGRPLGYILLVHDLGFMSRREAATVSWLLTAFVLLSVLASIVTLAAARWAWRS
                     WTSELQRALAGQSTPEFQPLMREVRTLVERLSAERETETRAGPWNPERLRAVLARHLS
                     GGRVVVLANREPYVHERGPSGVQVLHPASGLVTALEPVMRACSGVWVAHGSGSADRET
                     VDAHDRVRVPPAEESYWLRRIWLNEEEERGYYYGFSNEGLWPLCHVAHTRPIFRSEDW
                     EQYVRVNRKFAEAACAEADTVDPIVLVQDYHFALAPRMIRERLPQATIVTFWHIPWPN
                     AERMGICPWATELVSGLLGSSIIGFHTQQHCNNFVDTVDAYMESRIDRESQAIVQSSQ
                     RTLVRPYPISIDWPVHWLKDFPSARECRGVVRAELGLAEDAMLGVGVDRLDYTKGIEE
                     RLLAVDSLLTHYPEYRDRFVFAQLAAPSRTKIDPYRELNARVEALAERINDKWRNQRY
                     QPIVLLRQHHEPRAVFRFFRAADLCYVSSLHDGMNLVAKEFVAARDDELGVLVLSQFT
                     GAARELTEALIVNPYDIQQAGRALATALQMRPTEQRERMRAMRRMVSEFNVYRWAGKM
                     LTDAAELRRRARVTGQLGKRPGVGWLDTPLPRRRAL"
BASE COUNT          774 a         1238 c         1157 g          757 t
ORIGIN      
        1 ctttgccgat ggccgatacg agtcgcgcgc tcccttattc ggaggttccg cagcaaaaca
       61 gctaagagac atcgaccaca gcgtggtgcc aagccccacc gacccagggc atgcgtacct
      121 cgtgacgagg gtgcccgtgc gcgagcacgc gcaccttctc ggaatcatcg aaatcgccga
      181 gacactcgac atgcgcgacg acttcgttcg aaccagcgct tggaacaccg cgatcgctac
      241 cttggccctc gtgctcgtct ccgggatggt cgcgctagca gtgggagtag tcatggtcgg
      301 caggcctttg cgcctgctgg ccaacaaggc caagcgcgta ggcatcggtg acctaacggg
      361 tccgctgcat ctcaaacaac gtgacgagat cggcgaactc gcacgagagg tgaatgccat
      421 gtgcgagaag ctggccgagg caaacgagcg ctccgcgctc gaaacacgag cgcgattgaa
      481 agctctcgag caactccggc atgcggaccg gatcatcacc gttggccgtc tcgcagcagg
      541 tattgcacac gagctaggga ccccgctcaa cgtggtagcg gggcgcgtga agatgctgcg
      601 gcggtcccga gcagaaccac cgatgctcga tgaatacttg accatcatcg ccgaacaggt
      661 cgagcgcatg gcgctgatca ttcgccagtt gctggatttt gcgcgtcgtc gagaaccgcg
      721 tatccacacg cacgatctgg tcgctattgc aggtacgatc gcacggttgc tcgagcccat
      781 agcgcgacgc aagtcggtgc aggtcacgct aaccactacg aaccccgttt tggcccgcat
      841 cgacgcggcc cgcatcgaac aggtcatctc gaacctcgta gtcaacgcca ttcatgcctg
      901 tgcggctggc gggcacgtcg agatatcctg tggtctccag aacgacgaag gtgagttacg
      961 tcagcgcatc gcttacgtga aggttcgcga cgatggtcac ggaatggacg aggataccgt
     1021 cgcgcgtatc ttcgagccct ttttcacgac caaggacgtg ggacaaggga ccggactcgg
     1081 cctgagtgtt gcccacggta tcgtcgagga gcatggcggg cgaatttcgg tcgaaagcac
     1141 ccccggcgaa ggcagcgtct ttacggtcta cctgaccgca gacgaacggg ccgaagcgtc
     1201 ttgatcatga gtgtcgcagc ttcaatcgtt cccttccgct cgagctcccg cccagcgcgg
     1261 cgcccgaagt ttctgtctct atctcacatg cgcggcacta cagagcgcgc cgtcttggta
     1321 gtggcgtgtc gagccaaccg acaccgggac gcttacccag ctgacccgtg acgcgggccc
     1381 gcctccgcag ttcagccgcg tcggtcaaca ttttacccgc ccaacgatac acgttgaact
     1441 ccgaaaccat gcgacgcatg gcgcgcatcc gttcccgctg ctccgtgggt ctcatctgca
     1501 gcgccgtcgc cagagccctc cccgcctgtt gaatatcgta ggggttcacg atcaaagcct
     1561 cagtcagctc gcgtgcggcg ccggtaaact ggcttagaac gagaacgccc agctcatcgt
     1621 cgcgcgcggc cacaaactcc tttgccacca aattcatccc atcgtgcagg ctgctgacgt
     1681 agcagagatc ggctgcacgg aaaaagcgga acacggcgcg tggctcgtgg tgctgacgca
     1741 gcagcacgat gggttgatac cgctggttgc gccatttgtc attgatgcgc tctgctagcg
     1801 cctccacacg cgcgttcagc tcacggtagg gatcgatctt ggtgcggctc ggagcagcca
     1861 actgcgcgaa cacgaagcga tcccgatatt cgggatagtg cgtgagcagg gagtctaccg
     1921 ccaagagtcg ctcttcgatg cccttcgtat agtccagtcg gtcgaccccc acccccaaca
     1981 tagcgtcttc ggcaaggccc aattccgcac gaacgacacc tcgacactcc cgtgcagacg
     2041 ggaagtcttt cagccaatgt acgggccaat caatggagat cggataagga cgcaccagcg
     2101 tcctctggct gctttgtacg atggcctgcg attcacgatc tatacgactc tccatataag
     2161 cgtcgaccgt atcgacgaaa ttgttgcagt gctgttgagt atggaaaccg atgatgctcg
     2221 agccgagcag ccccgagacg agttcggtag cccatgggca aattcccatt ctttcggcgt
     2281 tgggccacgg gatatgccag aaggttacga ttgtagcctg cggaagccgc tcccgaatca
     2341 tcctcggcgc aagcgcaaag tgataatctt ggaccaacac gatcgggtcg acggtgtctg
     2401 cctcggcgca cgccgcctcc gcaaacttgc ggttgacgcg aacgtattgc tcccaatctt
     2461 cactgcggaa tatgggtcgg gtgtgtgcga cgtgacagag cggccacaag ccttcgttcg
     2521 agaacccgta gtagtagccg cgctcctcct cctcgttcag ccaaatgcga cgcaaccagt
     2581 aggactcttc cgcgggcggc acacgtacac ggtcgtgcgc gtcgacggtc tcgcggtcgg
     2641 cgctgccgct gccatgggca acccaaacgc ccgagcacgc gcgcatcact ggctctagag
     2701 cggtcacgag gccgctggcg ggatgcagaa cctgtacgcc ggacggcccc ctctcgtgga
     2761 catagggttc gcggttcgcc aggactacga cccgaccgcc tgataggtga cgcgccagca
     2821 ccgctcgcag tcgttccggg ttccacggcc cggctcgcgt ctccgtctca cgttccgcac
     2881 tcaatcgttc gacgagcgtg cggacctctc tcatcaaggg ctgaaactcc ggagtcgatt
     2941 gacccgctag cgctctctga agttcgctcg tccagctgcg ccatgcccac cgggcggccg
     3001 cgagcgtcac gatcgatgcg aggacagaga gcagaacgaa cgccgtcagc agccagctta
     3061 ccgtggccgc ctctcttcga ctcatgaacc ccaggtcgtg caccaggagg atgtagccga
     3121 gtgggcgccc ctcgctttcg agactcgtgg cgctcacttg cagatgccct agggatgatt
     3181 cgctcgtcat ccaccaggtc cgattgtgtg acgcgacctg gctcatgctc ccattgagca
     3241 cgactgaaca tgaaaattcg ctcgggaagt gctccgtcgc ggtcagaacc tcggcggtag
     3301 gcgagcaagc tgcagccccg atgacgcgct cgtcacgcgc gatattggtc aggctctctc
     3361 gaagcttgtg cgggtcagtc caatttgcgg cgaggctctg ccccgccgcg ctgactgcca
     3421 actcagtgcg cagccgcaag tccttctgga accattcgcg cgtagtgccg gtaatccccc
     3481 agtaggcgag catcgtgagc accccgaggc ccaccagcag caccaaaaga aagcggagcg
     3541 cgcgtcgcac cttcacgcag taccatatct gaacgcggtg aagcgtgctt cttcggagtt
     3601 gcgattgacc aacttcatcc tgggtcattc tgacccactc cgagcgggct caacgcgatt
     3661 attcacagtc gcagccgctg actccgcttt tgatgttgta atgttgtagt cagcgaggca
     3721 tgacccaatg tcgattggat ctgacgcgct gaagattgat catgagcgat tgggcttggg
     3781 cttcaccggt tcgcgccaag tcgatggctg gacgctttgc gcccgcgctg ttgctggcgc
     3841 atccagattg ttccgtgtcg ttggccattc gagaggaact gcttgaactg ggttacgggt
     3901 gttgcgttgt acgggaggcg acgcaa
//
LOCUS       DASTKU010000524        17968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_324026, whole genome shotgun sequence.
ACCESSION   DASTKU010000524 DASTKU010000000
VERSION     DASTKU010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 17968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17968
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324026"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFQ61_18045"
     CDS             complement(<1..479)
                     /locus_tag="VFQ61_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956419.1"
                     /translation="MTDVQFLARCMLLTGTAMTQKLRYPRSLSRLFTSAVALVWVLPA
                     CSGSNTGPGAPPGTPGTPEDGNFVSAVPDSASGPTRGGVASGAGGGTGAPNPSTPPLA
                     TDGNTNGEAARAIEEADVIKIEGTRLYALSQYGGLSVIDISKRDQLRLLGRHKIAAMP
                     "
     gene            755..1501
                     /locus_tag="VFQ61_18050"
     CDS             755..1501
                     /locus_tag="VFQ61_18050"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9956420.1"
                     /translation="MTPVPSGHKFCGRCGAAVPDNVMHLESHFYSDMQDPSKARLILI
                     RGDGMDGLSYHLKADQHVVGRQGQVEFPDDPFVSPRHANFFYRDGRLVVRDEGSINGV
                     YFRVRGAVDLAPGDMFLAGEQVFRLDPTPRPSDAADPDGTFFYSSPRFPSSFRLNQIL
                     EGGAIGMTVCARGNVLRVGREDGDLNFPGDFYLSTKHCSVEERDGRFQLVDHDSRNGT
                     YVRVKAEKPLSQGDYVFIGRRLLRVELNSN"
     gene            complement(1559..2848)
                     /gene="pcnB"
                     /locus_tag="VFQ61_18055"
     CDS             complement(1559..2848)
                     /gene="pcnB"
                     /locus_tag="VFQ61_18055"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HET9956421.1"
                     /translation="MPSSPPPDFESTSEEVFEESRSVDAPSSPIPQVYQVDLDESLID
                     PDAAKVVRRLARQGHEAYLVGGCVRDLLVERRPKDFDVATSARPDDVRRLFRNSRIIG
                     RRFRLVHVLFGGGKVIETATFRRAPEESTGRDGDDLLIRNDNVFGEAHEDAQRRDFTI
                     NGLFYDLERHEVIDWVSGMPDIERRSVHTIGNPMVRFLEDPVRILRAIKFSARLDFGI
                     SPEVYDAIVSCRFALKRAAKPRLFEEVLRLMRGGAAHRSFWIAWETGVLDVLLPELST
                     YLADRDEADDVVWRLLSELDRRTAEGLVPDDTLLWAALLLEPLREACEGTRDRVEAAA
                     EFLEPIIDRLNVPRRIADPVRRVIAILPRLESGRGGRFTRSGLYPLAAELASLRASCL
                     DDSAADEESTFVESGEEAAAAPARRKRRRRRRPRAEA"
     gene            complement(3066..5108)
                     /locus_tag="VFQ61_18060"
     CDS             complement(3066..5108)
                     /locus_tag="VFQ61_18060"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET9956422.1"
                     /translation="MTRASWRYGLLLSGALLGSALLWPGNSHRQSPAASAGVNAPLAG
                     RANGGVVRDRNELDKAKAAPDTSVAGVGSAAIAPSPVTPSNGTSARPEAEVTGESCSQ
                     ARRLVLPSGSPSHLACSEARTIISEVHTRYAGPTQLDDVHEFSRLVAGWLDPHGLWSA
                     APDSPVARALEQRAAALLDEIRQEPSSNEPCAAALALGADLAKWVTELGRLYDRAVLV
                     TPRRGPARDRALASARLPAFQDDPVTEPARSLSRRLGERIGRFKAAFPELSDKLVETA
                     RARYFPPLSAEGFAEVVLAAAVRAYVPSLDPHGDWAPFEEEWSLYAGDPGLDGEPRLW
                     NEITRTALGVRVVGGAPEPLATGDLILSIDGVLLAGMPLEQAEQLGRLEPPNGKSRHV
                     NVLRRTGQFQELEVAFDDSGDPAEAAVPLETERVRYGDGSALVVRLSEVADGVGEALG
                     RVLAEARHSDVSGVVLDLRGNGGGSTDAAASVIGLFLPGAPLFPLASRGKLLEVMQAL
                     EPAKDERWDGPVAALVDGYTASAAEMIAGALSSYERGKVVGGRTFGKGCIQEYADDHI
                     RKGVLRVTTLLFALPDGTPIQRTGLAPDLLVPTRKAREHEADLIRTLPAYRGPDVRDR
                     TLVSSRPWPSAHGRVGPCSDHLVCATLSRLGVSQVASRAAASRRTSPRALSGSAPR"
     gene            5402..8206
                     /locus_tag="VFQ61_18065"
     CDS             5402..8206
                     /locus_tag="VFQ61_18065"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HET9956423.1"
                     /translation="MLTPKGLHSSSDSGLGESNPEPSGQITSEVETSNAQSVAVESAS
                     TTHDVIVVLGCRVPASGPLGGAAARRARRAAQAYREGLAPWILASGGKIWGGRSEAHA
                     FRSQLVEAGVPEQQVICELESLSTRGNAREVARLCAERAFRRIAVVTCEWHMPRALRA
                     FARHGVDAHPLPAPAPDSPRHRYLELVQSWLDAALFRLERGRAPRIVTWMGFALGIAS
                     GITAVLACQETRGMRSRDHDTNPSAVASASASAKAHLEHAAFAEGAALEANDLQIRRE
                     AARRGMSNPTDAQFTALLRALADADPIVLSRVALALGKHCAGREDVVVRALVVRAASF
                     IVQRGASLSAREADAAEALARALGGCESREAERSLVALLELPAYAEGAALGLGDLGAS
                     RGSLDEASYLRLLAAATRSDNPVPTALYLFSRVTPTDPEIAARGLQVARQCLARGPEC
                     RGSMRQFALRALAHAGDVATLSDMIHQAQTPALERAELIRDLGRQGQRGLPALWAAFR
                     ELVPRDLQTPLTRPLAEVLDVPTLLAALESLPSPSAPEPALDLLVRLTASAAEANQPA
                     TQVRLTSLRCGAARVLANADWAHSALAACPADTRIGKLTRLRVIDRGRFSGARRDAYL
                     ALLEDADATVRQAALRLVASHREFTDLDAPIQAALGSNSLGLIAAAAEFVSQHPSRLG
                     ASPVGLTDALRAAWSTTENAHNPGVQALLIDAIAALSVLPLLSQVEHACAQTNPTLRR
                     HAERALSTLGQGPHRCHAPPSTTAPNPSLPASADAHVELTRTADSASGQRDLSPIELE
                     FETDAGPLELRLDPEIAPAAVAHIASLVRNGFYDGIEIHRYIPGFAVQFGDPTGDGYG
                     GDPAEALSTEVSPGRFRALSVGLAESGEGTGKSQLFVLLTDAPHLDGTTTRLGKASSG
                     WELVVPGDRIRRARLRVSPP"
     gene            8560..9048
                     /locus_tag="VFQ61_18070"
     CDS             8560..9048
                     /locus_tag="VFQ61_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956424.1"
                     /translation="MRGAHNQPLWLNRRLPRNARIEFDAVTQSDEGDLKVEAWGDGRS
                     ASTTVSYSNATSYLFIFGGWKNTLHVLARRDEHGPDRAELEVDSSSEDPRQAAVEAGR
                     LYHWVIERADGKTVLFSIDGLELLSLVDDQPLSGQGHEYFAFNNWETPVCFDNLKLQP
                     LP"
     gene            9179..10687
                     /locus_tag="VFQ61_18075"
     CDS             9179..10687
                     /locus_tag="VFQ61_18075"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HET9956425.1"
                     /translation="MELDEVEAALDELEVRLERLRALYEQYFLGFEKLEPSVLRKDVD
                     RRIYVLRKAHIRNTGRRFKLQTIIQRYNTFQQYWQRVCREIENGTYKRHVLRAERNIG
                     PTDLLTAAARRRFGKQRQPNAETSADAAAAPDRASPGSDAPDTVQHRPSALPASTPSR
                     VAGAPATARLDSSVPPPTLPRPAPPAPPSRIAASLTAPSSIPPATQPSTSYPEDLRGG
                     LKAPTAPLSARANAPMPSARRSEPPKRANETRGFESLDLDMDFMGDWDPAATAPEQTP
                     KAAPAPKGLPASGGVNRRLAPPPKPGKDGETATPQKPKPLEAPKLEAPKLVAQKVEVQ
                     RAQPPQVELAKAVRPQTVEPQPEPARKSPRAPVPQEAPPPAAAAAPRVSPPAASPSPI
                     APSPPTPTSPEPSPSDKLERAKPAAAAASREAARKARAAPEPLTDDRLRDLHSRLVQA
                     SQNLNRKPTSYESLAKSLKETEAKLRAQHGNRRIDFEIVIKDGKPAVKPTVR"
     gene            complement(11421..13130)
                     /locus_tag="VFQ61_18080"
     CDS             complement(11421..13130)
                     /locus_tag="VFQ61_18080"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9956426.1"
                     /translation="MQCEACGHSNLEGARFCANCGALMPTGEKSADPLIGQTIGGRFR
                     VTGVLGEGGMGVVYVGEQHMGSNIRKVAIKTLHPHLSKDPSVLARFNRECGTVSQLEH
                     PNTIKVYDFGSTADGTLYIAMEFVAGRPLADVIRDEGSLSPERVVRIMRQVCGALDEA
                     HDKGIVHRDLKPENVILTSRRGETDVVKVLDFGIAARTESADAQREQKLTQQGMVLGT
                     PPYMSPEQFTGKALDARSDIYSLGVMTYEMLTGRLPFDAETPWEWATQHMTAQPKPFE
                     VAALDKNIPSAMKHAVLKSLAKERDQRQSSARDFFSELSDGGHVTLDAPPPVSPAVSA
                     HASTGTAAMDALPHFNAPPPVVAPARPITPHGVATPVTAPGRGRGSSGGKGLVIGLVT
                     LGGVLLVAIAVVFIRSRPTNDDVTTVPIDTGPVVGTTNPPPAPTAAPPPTANTEPTAV
                     AEQTPTAQPTTVTSNPPKQTTSTGSGASTAKQTGGGGGCDACSAAVASGNFGAAAAAV
                     RSCSDTTKQEACKRALFGKARAAAVAAAKNGDCGTAEKIVRAGESAGLPSGLNGALSK
                     CGR"
     gene            complement(13220..13900)
                     /locus_tag="VFQ61_18085"
     CDS             complement(13220..13900)
                     /locus_tag="VFQ61_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956427.1"
                     /translation="MLKRIALSCVISGAALACSARPPAPPPSGELSPGVVFPTALGEG
                     HGDEDEPATAASGSASAPSGPAPGPTPAAAAPAPSASGTLAAPPKLGFAPDPEPLRLS
                     TQWEYELEWRDSRIQILSNELKKLPQPIVTPRRFGRFAAELWIGRELIDRVRFDFPLL
                     AGEAPQSSTGTQPLFPTPDLSKGATTRVKIMLPASPRAVKMKIVDRATGATLEFPFPP
                     PTSPPANP"
     gene            complement(13894..14805)
                     /locus_tag="VFQ61_18090"
     CDS             complement(13894..14805)
                     /locus_tag="VFQ61_18090"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET9956428.1"
                     /translation="MSSPNQCWVVERAESLEQWLERQHSKPAFEEGRVFVDGRRALDL
                     TIIITPGSKLEIYAPRSGTPTARVLHRSGGWLFAEKPPGISTEPDKRGHSACVVSQLA
                     HELGIPARNIHALSRLDLGVSGVVLLALDEASRKRALAWRAAGQIRRRYVALGAGHPK
                     PEQGEWNDAIARGPADPRRQVTDSGKASQSRYRVACCLTEGSPVCLLALEPVTGRTHQ
                     LRVHASAHGAPLLGDPSYGGPRRWVFENGQVIALPRVLLHASWAKLPGLPRVMAPIPA
                     DFSRIWQQLGGSEEDLERAVEVTVSTC"
     gene            complement(14802..16421)
                     /locus_tag="VFQ61_18095"
     CDS             complement(14802..16421)
                     /locus_tag="VFQ61_18095"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HET9956429.1"
                     /translation="MPESARGYRDLDSAAAEKLSGLWVAAALGDAPSQPLTPLAWDLA
                     LEDADLLERALDRRGASLPRRSRRLVAVAGHAYHAFLPLVRASRELIPLQPWHLALAI
                     ACEARGEVERLFPARPRKISKRSLMLWVARLTRTLARLSARIVGHEQHASQHYRWLTE
                     MDLGILPDDALATTLRECWSICRASRRLEIEATLDLLNAYAALCALCETARLGDPMPL
                     AASALIPDPLGLPTATPTLSALAWASAARSGASDEMGQWQSFSAALHAHGPGERSLLA
                     PHFGERPTQLAALFQRLKQAGDGAFETRISRVRRLRSEELQHALELCGALGGGLLKTL
                     FALIGRFVSLRSRSHVVRSRTLGMLRTAVLDVDRRLKRLLHTRPSSVFFLRLKELLES
                     TLRPDPALLERVRTRREAFDAAAALAPPRAVLGRQALPDRATRSLTGVGLGGERAIGA
                     ACVATNLDTALSLEPSGVLVLRSLDPGLGPALLSASAIVTDVGGLTDEGVLLARSLGI
                     PLVIGTRDATAQLATGEKVTVDAREGRVWVS"
     gene            complement(16433..16828)
                     /locus_tag="VFQ61_18100"
     CDS             complement(16433..16828)
                     /locus_tag="VFQ61_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956430.1"
                     /translation="MLSEFAPDFEPGLAWLGVAPKDILHFGMAADASDTLPLVEALSL
                     NSSKAGEARTFEDQGSAGPAREPSVQNYLDQKVNAALRALPTGLSDEEVGFLRRCLLS
                     ELREDWALRAWVGRALSEEPVVDQDAGQE"
     gene            complement(16818..17678)
                     /locus_tag="VFQ61_18105"
     CDS             complement(16818..17678)
                     /locus_tag="VFQ61_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956431.1"
                     /translation="MFSGQSSRVAAQFCTAFATLAFSALASAQTTGAFPSAEPAPSPA
                     PENERIAPTRKVDPRQRPGAANSEGQRGAPAAAPSPSVESNRSSSGAASPKREVVPGS
                     LPADPALLDVDDAGPSAQRALPRRSARLGLPPLLPYRSGLPVPRGYHVEYRPATGLIL
                     SGALALGVGYATAIGIGMNRDFEGSLGWLAVPVLGPWPALAGRKLSCAATTVDEARGC
                     LDRAYEEATTIALVAVDGMLQATGALLLIAGLASGRSELVRSADRSTVSFSIRRRPEG
                     GVQLGVLGAF"
BASE COUNT         3298 a         6037 c         5698 g         2935 t
ORIGIN      
        1 ggcatggccg cgatcttgtg ccgcccgagc aagcgcagct gatcgcgctt cgagatatcg
       61 atcacgctca agccgccata ttgcgagagc gcatacagcc gggtgccctc gatcttgatg
      121 acgtccgcct cctcgatagc gcgcgcggct tcgccgttgg tgttgccatc ggtcgccaaa
      181 ggaggcgtgg aggggttcgg cgctccggtt ccgcctcccg cgccgctcgc gacaccgccc
      241 cgggttggac cgcttgcact gtcggggact gcggagacga aatttccgtc ctcaggggtc
      301 ccgggtgtgc cggggggcgc gcccggaccg gtgttgctcc cggaacaggc ggggagaacc
      361 cacaccagtg caaccgctga ggtgaagagc cgagacaggg accgtgggta gcgcagcttc
      421 tgggtcatgg cagtgccagt gaggagcatg caacgtgcca gaaactgaac atccgtcatt
      481 tggcaatctc gtttgaattt gccttgaatt gcgccggctc gcagaccagt gtgccaaaca
      541 tctgagccag gtcggcacag cctctggcgc gccggtcagc cgtcggtacg catgagcaaa
      601 gggtgatacg agcaggccct ccctcttgcc caaccgagcg aaattgttgg agcctgacgg
      661 ctcgtcggct acgaaacgga gactcggtgc gcccgcttcg gcgcagagcg gagacttcat
      721 ggatcaagct cggtacttcg tatgcgaatc gtgcatgacc ccggtgccca gcggtcacaa
      781 gttctgcggg cgttgcggtg cagcggtgcc cgacaacgtc atgcacctcg agtcgcattt
      841 ctacagcgac atgcaggacc cctcgaaggc gcggctcatt ctgatccgtg gagacgggat
      901 ggatggtctc tcctaccatt tgaaggcgga ccagcacgta gtgggtcgcc aaggccaagt
      961 ggagttcccg gatgacccgt tcgtgtctcc acggcacgcc aacttctttt atcgcgacgg
     1021 acggctcgtc gttcgcgacg aggggtcgat caacggtgtc tacttccggg tgcgtggtgc
     1081 agtcgatctc gcgccggggg acatgttcct cgcgggcgag caagtgttcc gtttggatcc
     1141 gactccccgc ccctcggacg cggccgatcc tgatggcacc ttcttttact cgtcccctcg
     1201 cttcccctcg agcttccgcc tgaatcagat cctcgaagga ggagcgatcg gcatgacggt
     1261 gtgcgctcgc ggcaacgtgc tgcgcgtcgg acgtgaggac ggcgacctga atttcccggg
     1321 cgatttctat ctctccacga agcactgctc cgtcgaggag agagatggcc gtttccagct
     1381 cgtcgaccac gacagccgca acggcaccta cgttcgcgta aaagccgaga agccgctcag
     1441 ccaaggagac tacgtcttca ttggccgacg gctgctgcgc gtcgagctga actccaactg
     1501 agcgccagac gaacgagcgc tgggcggcgc gcataacgct gcgctgcccg gcatcgcttc
     1561 aggcttcggc gcgcgggcgg cgtcgacgac ggcgcttgcg acgtgcgggt gctgcggccg
     1621 cttcctcgcc gctctccacg aaggtggact cttcatcagc tgccgaatca tccaggcagc
     1681 tcgcccgcag agaagcgagc tcggccgcga gcggatacag ccccgagcgt gtgaagcgac
     1741 caccgcgtcc ggattcgagc cgcggcaaga tcgcgattac gcggcgcacg ggatcggcga
     1801 tccgccgggg cacattcaag cggtcgatga tgggctccaa aaactccgcc gccgcctcga
     1861 ctcgatcgcg cgttccctcg caagcctcgc gcaaaggctc gagcaggagc gccgcccaca
     1921 gcaacgtatc gtcggggacc agcccctccg ccgtgcgacg atccagctcg gatagcagcc
     1981 gccaaaccac gtcgtccgcc tcgtcgcgat cggcgagata cgtagagagc tccggcagca
     2041 acacgtccag cacacccgtc tcccacgcaa tccagaacga gcggtgcgcg gcgccacccc
     2101 gcatcaagcg cagcacttct tcgaacagac gcggcttggc ggcgcgcttg agcgcgaaac
     2161 ggcaactcac gatggcatcg tagacctcgg ggctaatccc gaaatcgagg cgggcgctga
     2221 acttgatcgc ccgcaggatc cgcaccggat cttcgaggaa ccgcaccatc ggattgccga
     2281 tcgtgtgcac gctccttcgc tcgatgtcgg gcatgcccga cacccaatcg atcacctcgt
     2341 gtcgttcgag atcgtagaag agcccgttga tcgtgaagtc gcggcgctgg gcgtcttcgt
     2401 gagcctctcc gaacacgttg tcgttgcgga tcagcaggtc gtcgccgtca cggccggtcg
     2461 actcctccgg ggcgcgccgg aaggtcgccg tctcgatgac tttgccgccg ccgaatagga
     2521 cgtgaaccag gcggaagcgc cgcccgatta tccgtgaatt gcggaacaag cgacgcacgt
     2581 cgtcaggacg agcgctggtc gcgacgtcga agtccttggg tcgacgctcg acgagcagat
     2641 cacgcacgca gcctccgacg agataggcct cgtgcccctg acgggccaag cgccgcacca
     2701 ctttggcggc gtcgggatca atcaaggatt cgtcgagatc gacctggtac acctgcggga
     2761 tgggagaact cggtgcgtcg acagaccgtg attcttcgaa cacttcctcg ctggtagatt
     2821 cgaagtcagg agggggcgaa gaaggcatga tagacagaat cagaggcgcg agaattcgga
     2881 ctcgttttag ctcggctcgc ccccatcgca aagcgggtct ttgttcgaga ggccgggagc
     2941 gaccatcgct cgggtataat ggctgccggt cgaaggcaac cgcgaaagcc ccgtaccgag
     3001 ggccgagcgg ggccgagatg cggatgccga tcggcgccga accgtgccgc gctaacgcgg
     3061 ccgtatcaac gcggtgcgga gcccgacaga gcccgcggcg acgtacggcg cgatgccgca
     3121 gcacgcgaag caacttggga cacgcctaga cggctcagcg tggcgcacac caaatgatca
     3181 ctgcacggcc ctacccgccc gtgcgcggac ggccaaggac ggctactcac cagggtgcgg
     3241 tcccgcacgt cgggcccgcg gtaagccggc aaggtccgaa tcaggtctgc ctcgtgctca
     3301 cgggctttcc gcgtgggcac cagcaagtcc ggagccaacc cagtgcgctg aatcggcgta
     3361 ccgtcgggca gtgcaaagag cagggtggtg acgcgcagga cacctttgcg gatgtggtcg
     3421 tcggcatatt cctggatgca gcctttgccg aaggtgcgac cacccaccac tttgccgcgc
     3481 tcgtagctgc tcaaggctcc agcgatcatc tccgcagcgc tcgccgtgta accgtccact
     3541 aacgccgcga ccgggccatc ccagcgctcg tctttggcgg gctcgagcgc ctgcatcact
     3601 tcgagcagct tgccgcggga cgcgagggga aacagcgggg caccgggtaa gaacaggccg
     3661 atcacggacg cggctgcgtc cgtcgagccg ccgccgttgc cgcgtaaatc gaggaccacg
     3721 ccggacacgt cggagtggcg cgcttccgcg agtacgcggc ccaaagcctc ccccacgccg
     3781 tctgccactt cactcaagcg caccaccagc gccgagccgt cgccgtatcg gacgcgctcc
     3841 gtttcgaggg gtaccgccgc ctccgcagga tcgcccgagt catcgaacgc gacctcgagc
     3901 tcttggaatt gaccagtgcg tcgcagcacg ttgacgtgac gtgatttacc gttgggcggc
     3961 tcgagccgcc caagctgctc agcctgctcg agcggcatgc cggcgagcag cacgccgtcg
     4021 atggacagga tcaaatcacc agtcgccagc ggttctggtg cgcctccgac gacgcggaca
     4081 ccgagcgcgg tccgagtgat ttcgttccac agtcgtggtt caccatccaa acccggatca
     4141 cccgcgtaca gggaccactc ctcttcgaag ggcgcccaat cgccatgtgg atccaagctc
     4201 ggaacgtagg cgcgaacggc cgcggccaac accacctccg cgaacccttc ggcggagaga
     4261 ggcgggaaat agcgggcccg cgccgtctcc acgagcttgt ccgagagctc cgggaaagcg
     4321 gctttgaagc gcccaattcg ctcccccaaa cgacgcgaga gtgagcgagc aggttccgta
     4381 accgggtcat cctgaaacgc gggcaaacga gccgacgcca gcgcccgatc acgcgcaggt
     4441 ccacgccgag gcgtcaccag caccgcgcga tcgtagagcc gcccaagctc cgttacccac
     4501 ttcgccaagt cggcccccag cgctaaggcg gcggcgcacg gctcgttgga gcttggctct
     4561 tgccggatct cgtccagtaa cgcggccgcg cgttgctcca gggcgcgagc cacgggcgag
     4621 tccggtgccg cagaccagag accatgggga tcgagccaac cggccaccag acgggagaac
     4681 tcgtgtacat catcgagctg cgtgggcccc gcgtaacgcg tgtgcacctc gctgatgatc
     4741 gttcgagctt ccgagcaagc cagatgggaa ggactcccgc tcggcaacac cagccggcgc
     4801 gcctgcgagc agctctcccc cgtcacctcg gcttcggggc gcgcagacgt accgttcgaa
     4861 ggtgtaactg gtgatggcgc gatcgctgct gacccgaccc cggcaacgct agtatccgga
     4921 gctgcctttg ctttatcgag ctcgtttctg tcccgcacta caccaccgtt ggctcgaccc
     4981 gcgagcggcg cgttgacgcc cgccgaagcc gcgggagatt gacgatgtga gttgcccggc
     5041 cacagtaagg cgcttcccag gagggcgccc gacaataaca gaccatagcg ccacgaggcc
     5101 cgagtcattg aagacgagaa gctagagtat ctcggtttcc gttcgccgcc acgcaatttg
     5161 gtggcgcgca cgctcgagca ctaaacgatg ggctctgcgg gaaccaaccc atgggcttcg
     5221 acggcgaacc caccgctccg tcaaccgggc cgaaaggttt ggaggaccaa gatggtcgag
     5281 gcttccttct ttgctggatt tctctgcgag ttttctgtcc tggtctgctc gaaagcgctt
     5341 gaatcgcggc tccctgcggc acagtcgtcg tgcagcgcct ctccaagcac agtcatctcg
     5401 aatgctcacg cccaagggtc tccactcttc gtctgactca ggcctcggcg agtccaaccc
     5461 ggaaccgtcg gggcagatta cgagcgaagt tgaaaccagc aacgcgcaga gtgtggcggt
     5521 cgagagcgcg tccaccacac atgacgtgat cgtagtcttg ggctgtcgcg tgcccgcctc
     5581 ggggcctctc ggcggggcag cggctcggcg tgcgaggcgc gccgcacagg cttacagaga
     5641 gggcctggca ccgtggatcc tcgcgagcgg tgggaagatt tggggcggtc gtagtgaggc
     5701 gcatgctttc cgctctcagt tggtcgaagc tggggttccg gagcagcagg tgatttgcga
     5761 gctcgagtcg ctcagcacgc gcggcaacgc gagagaggtc gctcgtttgt gcgcggagcg
     5821 cgccttccgt cgaatcgcgg tcgtgacgtg cgagtggcat atgccgcgag cgctgcgtgc
     5881 ctttgctcgg cacggagtcg acgctcaccc gctgcctgcc cccgcaccgg attcaccgcg
     5941 acatcgctac ctggagctgg tgcaaagctg gctcgacgcg gcgttgtttc gcttggagcg
     6001 cggccgggcg ccccgcattg tgacgtggat gggtttcgcg ctcggcatcg cgagcggcat
     6061 cacggcagtg ctcgcgtgcc aggagacgcg cgggatgcgc agccgcgacc acgatacgaa
     6121 tccgagcgca gtcgcgtctg cgagcgcctc ggccaaggcg cacctcgagc acgctgcgtt
     6181 cgcagagggc gcggcgctgg aggccaatga tctgcagatc cgcagagaag ctgcccgtcg
     6241 cgggatgagc aacccgaccg acgctcaatt caccgctcta ctcagggctc tggccgacgc
     6301 ggatccaatc gtgctttcga gggtggcctt ggcgctaggc aagcactgcg cgggacgcga
     6361 ggacgtggtc gtacgtgcgc tcgtcgttcg tgccgcgagc ttcatcgtcc agcgcggtgc
     6421 ctccttgtct gctcgagagg ccgacgcagc tgaagccctg gcccgagcgc tcggcggctg
     6481 cgagagccgc gaggcggaac gctcgctagt ggcacttctc gaactgcccg cctatgcaga
     6541 aggggctgcg ctaggcctcg gtgacctggg cgcgagccgg ggcagcttgg atgaagcgag
     6601 ctacttgcgc ctgctggcgg ctgccacccg atcggacaat cccgtgccga ccgcgctcta
     6661 cctgttttcg cgggtcactc cgaccgatcc ggagattgct gctcgcggcc tgcaagtggc
     6721 gcgacagtgt cttgcgcgtg gccccgaatg tcggggttcg atgcgccagt tcgcgttgcg
     6781 cgcactcgct cacgccggag atgttgccac cttatccgat atgatccatc aggcgcagac
     6841 gccagcactc gagcgcgcgg agctgattcg agatctcggc cggcaagggc agcgcgggct
     6901 ccccgcgctc tgggcggcat ttcgcgagct ggtgcctcga gatttgcaga ccccgctcac
     6961 tcgcccacta gccgaagttc tcgacgtgcc gacgctgctc gcagcgctgg agtccctgcc
     7021 cagcccgtct gcacccgaac ccgccttgga cctcctagtt cggctgacag cctcagccgc
     7081 ggaagccaac caacctgcaa ctcaggtgcg tttgacgtcg ttgcgctgcg gcgccgcgcg
     7141 ggtgttggcc aacgccgatt gggctcactc cgcgttggcc gcgtgccccg cggatactag
     7201 gatcggcaaa ctgacgcgtc tcagagtcat cgaccgggga cgtttttccg gcgcgcgacg
     7261 cgacgcttat ctggccctgc tagaggacgc cgatgcaacg gttcgacagg cagccctccg
     7321 gctcgtcgcg agtcaccgcg agttcaccga cttggatgcg cccatccaag ccgcgctcgg
     7381 gagcaactcg ctggggctga tcgccgccgc cgctgagttc gttagccaac atccaagccg
     7441 actgggagct tcccccgtcg ggctcacgga cgcgttgcgg gccgcatgga gcacgacgga
     7501 aaacgcgcac aacccgggcg tgcaggcgtt actgatagat gcaatcgcag cgctttcggt
     7561 tttgccgcta ctctcccagg tagagcacgc ctgcgcgcag accaatccga cgctgcgtcg
     7621 acatgccgag cgtgccctct cgacgctcgg ccaaggccct caccgctgtc acgcgccgcc
     7681 ctcgacgacc gctccgaacc cttccctccc cgcaagcgcg gacgcgcacg tcgagttgac
     7741 tcgaacggct gactccgctt ccgggcaacg tgatctctcg ccgatagagc tcgaattcga
     7801 gaccgatgcc ggcccgctcg agctgcgctt ggacccggaa atcgctcccg cagccgtagc
     7861 tcacatcgca tcgctcgtgc gcaatggatt ttacgatggc atcgagatcc accgttacat
     7921 cccgggtttt gcggtgcagt ttggtgaccc gactggcgac ggctacggcg gagatcccgc
     7981 cgaagcccta agcaccgagg tgagcccggg ccgctttcgt gcactgagtg tagggctcgc
     8041 cgagagcgga gaaggcaccg gtaagagcca actattcgtg ttgttgaccg acgcaccgca
     8101 cctggacgga accaccacgc ggctcgggaa ggcgagctcc ggctgggaac tcgtcgtgcc
     8161 gggggatcgc atccgtcggg cgaggctgcg cgtgagccct ccgtaacctc gaaaggagcc
     8221 aaggccatgc atcggcagaa gagctcgcag tccattcgag tagcgaggtg gcgagccgcc
     8281 tcatgctcgg tgtcattgct gatcgctctc ggttgcttgc cagagaaccc ggatccagcc
     8341 gatcgcgcct cgatcaagcg tccggtgcgg agcagctctg gcccacgctt ggcgacgccg
     8401 cacgtgcccc gtgcgcgccc tcgcgtacga cccataaatt ccgcgacgat cgcatccgga
     8461 tctcctcccg cttttttcga cgacttcgag cgggcctcgt tgggcgcgac gtacgcttca
     8521 cggtccgccg cttggcatct aaaccaggga aggctgtgtg tgcgcggcgc acacaatcag
     8581 cccttgtggc tcaatcgacg gcttccgcgg aacgcccgta ttgaattcga tgccgtcacc
     8641 caatccgacg aaggcgatct caaggtcgag gcttggggag atggtcgaag cgcgtcgacc
     8701 accgtgtctt actccaacgc caccagctac cttttcatat tcggcgggtg gaagaacacg
     8761 cttcacgtgc ttgcccgccg tgatgaacac ggacctgatc gcgcggaact cgaggtggat
     8821 tcgagctcgg aggaccctcg acaagcagcc gtcgaagccg gccggcttta tcattgggtg
     8881 atcgagcgcg ccgatggcaa gacggtcctg tttagcattg atggactcga gctcttgagc
     8941 ttggtcgacg accagccgct tagcgggcag gggcacgagt acttcgcttt caacaactgg
     9001 gaaacgcccg tatgtttcga caatctgaag ttacagccgc tcccctgaag agcttggagt
     9061 ccgacgggcc gaacatgccc tctatcccgc cgaggggatt ccgcccgatg gctgggtgag
     9121 cacccgtgtg tgaggctagg cactagacgg atcaacgaag cgaggatcgg acaagaacgt
     9181 ggagttggat gaggtcgaag cagcactgga cgaactcgag gttcgactgg aacggctgcg
     9241 tgctctgtac gagcaatact ttctcggctt cgaaaaactc gaacccagcg tgctgcgcaa
     9301 agacgtcgac cgacgaatct atgtgcttcg caaggcccac atccggaaca ccgggcggcg
     9361 cttcaagctg cagacgatca tccagcgcta caacacgttc cagcaatatt ggcagcgcgt
     9421 gtgccgtgaa atcgagaacg ggacgtacaa gcgccacgtg ttgcgggcag agcgcaacat
     9481 cggccccacc gatttgttga cggccgcagc gcgccggcgt tttgggaaac agcgccagcc
     9541 gaacgcagaa acgagcgcgg acgcagcagc tgctcctgat cgcgcttcac ccggctcgga
     9601 cgcgcctgac accgttcaac accgtccgag cgcactgcca gcatcgacgc cgagccgcgt
     9661 ggcaggcgcg cccgccaccg cacgcctcga cagcagcgtg cctccgccta ctttgccgcg
     9721 gcccgcgccg cctgcaccac cctcgcgcat cgcggcctcg cttacggccc cctcgagcat
     9781 cccccccgcg acccaaccca gcacctcgta tccagaagac ctgcgcggag gcttgaaagc
     9841 gcccactgct ccactgtccg cacgagccaa cgcgccaatg ccatctgccc gaagaagcga
     9901 gccccccaag cgagccaacg agacgcgcgg tttcgaatcc ctcgatctgg acatggattt
     9961 catgggggac tgggatccgg cagctacagc accagaacag accccgaaag ccgcgccggc
    10021 acccaagggg ctgcccgctt cgggtggcgt caatcgtcgt ctcgctccgc cacctaagcc
    10081 ggggaaggac ggggagaccg cgacgcccca aaagcccaag cccttggagg cgccgaagtt
    10141 ggaggcgccg aagttagtgg cgcaaaaggt cgaggttcaa agagcgcagc cgccacaggt
    10201 cgagctggcc aaagcggtgc ggccccaaac agtcgagccg cagccggaac ccgcgagaaa
    10261 atcgccgcgc gcgccagtgc ctcaagaagc cccgccgccc gccgcagctg cggctcccag
    10321 ggtatcgccc cccgcagctt cacccagccc gatcgcgcct tcacctccta cgccgacgtc
    10381 accagagcca agcccgagcg acaagctcga acgagccaag cccgcggctg ccgccgcctc
    10441 gcgcgaagcc gcgcgcaagg cgcgagcggc accggaaccc ctcaccgatg atcgtcttcg
    10501 agacctgcac tcccggctcg ttcaagccag ccaaaacttg aaccgcaagc caaccagcta
    10561 tgagtcgctc gcgaagtcac tgaaggaaac ggaagccaag ctacgagcgc aacatggcaa
    10621 ccgtcgaatc gacttcgaaa tcgtgatcaa agacggcaag cccgccgtga agcccacggt
    10681 gagatgagca agccccaacg gggcaacgcc caactggcga gcaggcactc ccgccagggc
    10741 tgtgaccgcc tccacctctc aaccacaccc ctctgctcct gcatccaccc actccaccac
    10801 gctgaacccg cgaccctggg gcgtcgctga gctacgacgc ccgttgcggg tgcagccgcg
    10861 gtaaacgcgc tgcggcgcta tccgaagaga cgacgcgccg gggctggcga ctgcggagcc
    10921 ggcgctgccg gctcaacgaa gacgtccccg tcgcagaaca ggatggcgca ggcactacgg
    10981 cccgcttctc gcatcctggc gaagagctcc gggagctcgg gatccgaatc gaccacgtca
    11041 ccctcaacag gctgtcgggt aacgtgatcg tatcgacagt tatcgatggc gacccacagg
    11101 ccgcggaact tatccgaccg gcacaactcc ggccaggtca tcctgatggt catcgtttga
    11161 tgcgcctttt cctgagcatc cctctctcag ggtgcacacg gcccggaaac gcgaaatggc
    11221 tgttcgaaac gggctgcacg ggaataggac ctgagcggga tatcaataca agttcccacg
    11281 gccgtcaagc tatcaccaca atgcgctgcg tcacaaaaat tccgctttga aatcgggcta
    11341 ttgcggaggg ggtagggaat tgacccgccg attctgaaca ctcgtacaga cactacgagt
    11401 cacgcaatcg accccgtttc tcaacgcccg cacttgctga gcgccccatt caaaccactg
    11461 gggagacccg cgctctcgcc tgcgcgcacg atcttctcag ccgttccaca atcgccattc
    11521 ttggcggcgg ccaccgcggc cgctcgagcc ttgccgaaca acgcgcgctt gcacgcttcc
    11581 tgtttagtgg tgtccgaaca gctgcgcact gccgccgcag ctgcgccgaa attgccgctc
    11641 gcaactgccg ccgagcaggc gtcgcaaccc ccacctccgc cggtctgctt tgcggtgctc
    11701 gcgcctgagc cggtagaagt cgtttgcttc ggcgggttgg acgtaacggt cgtaggttgc
    11761 gctgtcggtg tttgctccgc gacggcggtg ggctcggtgt tcgcggtagg cggcggagcg
    11821 gccgtcggcg cgggcggcgg atttgtcgtg ccgacgaccg gtcctgtgtc gatcggtacg
    11881 gtggtcacat cgtcgttcgt ggggcgcgag cgtatgaaca cgacggcgat cgcgaccaac
    11941 agcacgccgc ccaacgtaac cagcccaatc accaggcctt taccgccgct gctgccgcgc
    12001 ccgcgtccgg gtgcggtcac gggagtcgcc actccgtgcg gagtgatggg tcgagccggc
    12061 gccactacgg gagggggcgc gttgaagtgc ggcagggcgt ccattgcagc ggtgcccgtg
    12121 ctggcgtgag ccgacacggc tggagacacc ggaggaggtg cgtccaacgt tacgtgaccg
    12181 ccgtcggaga gctccgaaaa gaagtcacgc gccgaactct gccgctggtc gcgctctttg
    12241 gcgagcgact tcagtacggc gtgcttcatg gcggagggga tgttcttgtc gagcgcagcg
    12301 acttcgaacg gcttgggctg tgccgtcatg tgctgggtcg cccactccca gggagtttcc
    12361 gcgtcgaacg gcaagcgccc ggtcaacatc tcgtacgtca tcacgccgag cgaatagatg
    12421 tcgctccggg catccagagc tttacccgta aattgctctg ggctcatgta gggcggtgta
    12481 ccgagcacca tgccttgctg agtcagcttc tgttcacgct gagcatccgc tgattcggtg
    12541 cgggcggcga tcccgaagtc gagcactttg accacgtcgg tctcgccgcg acgactcgtc
    12601 agaatgacgt tttccggctt caaatcgcgg tgcacgatgc ccttgtcgtg ggcttcgtcg
    12661 agcgcgccgc acacctgcct catgatgcgc acgacgcgct ccgggctgag cgatccttcg
    12721 tcgcggatca cgtctgcgag cgggcgcccc gccacgaact ccatcgcgat gtacagggtg
    12781 ccgtcggcgg tcgaaccgaa gtcgtaaacc ttgatcgtgt tggggtgctc gagctgcgag
    12841 accgtgccgc actcccggtt gaagcgcgcg agcaccgacg ggtctttgga gaggtgcggg
    12901 tgcaaggtct tgatcgcgac cttgcggatg ttcgagccca tgtgctgctc gccgacgtag
    12961 accacgccca tgccgccctc accaagtaca ccggtgacgc gaaaacgtcc gccgatggtt
    13021 tgcccaatca gcggatcggc cgacttctca cccgtaggca tcaatgcgcc gcaattggcg
    13081 cagaagcggg ccccctcgag attactgtgg ccacaagcct cgcactgcat cggggcaagc
    13141 ctatgccaga ccccgagccc gacgctagcc ccgagccacc gattcgtgga agcgccggcc
    13201 cgcccgcacg aaacgtggac tacggattgg ctggcggaga cgtcggcggt gggaacggga
    13261 actccaacgt cgcacccgtt gctcgatcca cgatcttcat cttcacggcg cgcggcgagg
    13321 ccggaagcat gatctttacc cgcgtcgtgg ctcccttgct caagtccggc gtggggaaca
    13381 gcggctgggt acccgtcgag ctctgaggtg cctcgcctgc cagcaagggg aaatcgaagc
    13441 ggacgcggtc gatcaactcg cgaccgatcc agagctcggc cgcaaatcga ccgaaacgac
    13501 gaggtgtgac gatgggctgg gggagcttct tgagctcgtt cgagaggatc tgaatccgcg
    13561 agtcacgcca ctcgagctcg tattcccatt gggtagatag gcgcaaaggc tcgggatcag
    13621 gagcgaagcc gagcttgggg ggtgccgcga gcgttccgct ggcgcttggg gcaggcgcag
    13681 ccgcagcggg ggttgggccg ggagcgggcc cggatggcgc ggacgcgctc cccgacgctg
    13741 cagtagcggg ctcgtcttcg tctccgtgtc cttcgccgag ggcggtcggg aacacgaccc
    13801 ccggcgatag ttcgccactc ggcggaggag cgggtgggcg cgccgagcaa gccaatgccg
    13861 ccccggagat cacgcacgag agagcgatcc gcttcaacac gtgctcaccg tcacctctac
    13921 ggcgcgctcg aggtcctctt ccgaaccacc aagttgctgc caaattcgag aaaaatctgc
    13981 gggaatgggt gccatgactc gcgggaggcc gggcagcttg gcccaggatg cgtgcaacag
    14041 cacgcgcggt agggcgatca cttgcccgtt ctcgaacacc cagcggcgag ggccaccgta
    14101 gctcgggtca cccaacaagg gagccccgtg cgcggaagca tggacacgca actggtgggt
    14161 acgcccagta acgggctcca acgcgagcag gcatactggg gagccctcgg tcaggcagca
    14221 cgccacgcgg taacgactct gactcgcctt gccggagtcc gtcacttggc gtcgtgggtc
    14281 agccgggccg cgagcaatcg catcgttcca ctcgccctgc tcgggtttgg gatgtcctgc
    14341 gccgagggcc acgtaacggc gtcgaatttg ccccgcagct cgccacgcca acgctcgttt
    14401 gcgcgaggct tcgtccaaag cgagcagcac caccccggag acacccaaat cgagacgaga
    14461 caatgcgtgg atgttcctgg caggaatgcc gagctcatgc gcaagctgcg acaccacgca
    14521 ggcgctatgg ccacgcttgt caggctcggt cgagatgccc gggggcttct cggcgaacag
    14581 ccacccgccg ctgcgatgga gcacacgcgc ggtcggggtg cccgaccgag gcgcgtaaat
    14641 ctcgagctta cttccggggg tgatgattat cgtcaggtca agcgcgcgac gtccgtccac
    14701 gaacacgcgt ccctcttcga aggcgggctt cgaatgctgg cgttcgagcc attgttccaa
    14761 tgactccgcc cgttctacga cccagcattg gttagggcta ctcacgagac ccaaaccctg
    14821 ccctcgcgcg cgtccactgt caccttctct cccgtcgcga gctgcgccgt agcgtcccgg
    14881 gttccgatca ccagaggaat gccgagcgaa cgcgccagca aaacaccctc gtccgtgagt
    14941 ccgcccacat cggtgacgat ggcggaggca gacaagagcg ctggccctag ccctggatcc
    15001 agagagcgca gcaccagcac cccggatggc tcgagtgaga gcgccgtgtc gaggttcgtc
    15061 gcaacgcaag cggccccgat cgcacgctca ccgccgagac caacccccgt gagtgacctc
    15121 gtggcgcgat ccggaagcgc ttgtcgcccc aacaccgcgc ggggcggcgc cagcgcggct
    15181 gcggcatcga atgcctctcg tcgcgtacgc acccgctcga gcagagcggg gtccgggcgt
    15241 aaggtgctct cgagcaactc cttgagccgc aggaagaaca cactgctcgg ccgtgtgtgc
    15301 agcaagcgct tcaagcggcg atcgacatcc aggaccgccg tgcgcagcat gccaagggta
    15361 cgggagcgca ccacgtgcga gcgcgagcgt aagctcacga agcgcccgat cagcgcgaat
    15421 agcgtcttta gcaagccacc gccgagggca ccgcaaagtt cgagcgcgtg ctgcagctcc
    15481 tcggagcgca gccgccgcac acgggaaatg cgcgtttcga acgcgccatc cccagcctgc
    15541 ttcaatcgct gaaagagggc cgccagctga gtcggccgtt ctccgaagtg cggcgcaagc
    15601 aggctacgct ctccgggccc gtgcgcgtgg agggcggccg aaaaggactg ccactgcccc
    15661 atctcgtcgc tggcgccaga gcgcgccgcg gaagcccacg ccaacgcaga gagggtcggg
    15721 gttgcggtgg gcaaacccag cggatctggg atcaaggccg acgcggctag cggcatgggg
    15781 tcacccaagc gcgcggtctc gcataacgcg cacagcgccg cgtaagcgtt cagcaggtcc
    15841 agcgtcgcct cgatctccag tcgccgcgaa gcgcgacaaa tcgaccagca ttcgcgcagc
    15901 gtcgtcgcca gtgcgtcgtc cggaagaatg cccaaatcca tctcggtgag ccagcggtaa
    15961 tgctgggatg catgctgctc atggccgacg atccgcgccg agagccgtgc gagggtacgc
    16021 gtgaggcgag ccacccataa catcaagcta cgtttggaaa tcttgcgggg cctcgccggg
    16081 aacagccgtt cgacttcccc tcgtgcctcg cacgcaatcg ccagcgcgag gtgccagggc
    16141 tgtaacggga tgagctcccg cgaggcacgc acgaggggca agaacgcatg ataagcgtga
    16201 ccagccacgg ccaccagacg ccgagaacgc cgtggaagag aggcgccacg ccgatccaac
    16261 gcgcgctcca agaggtcagc gtcctcgaga gcgagatccc acgccagtgg agtcaaaggc
    16321 tggctcgggg cgtccccgag cgccgctgcg acccacaggc cgctgagttt ctcggctgcc
    16381 gccgagtcca gatcgcggta gccacgcgca ctctcgggca tgctttgcgc ccttattctt
    16441 ggcccgcgtc ctggtccacg acgggctctt cgctcagcgc acgcccaacc caagctcgga
    16501 gcgcccaatc ctctcgaagc tcactcagca ggcagcgccg caagaagccg acctcctcgt
    16561 ccgacaaacc cgtgggtaaa gcccggagtg cggcgttcac cttctgatcc agatagtttt
    16621 gtacgctcgg ttcccgggct ggtccggcag acccttggtc ttcgaaagta cgggcttcgc
    16681 cggccttcga ggaattcaac gagagagcct cgaccagagg cagagtgtcg ctcgcatcgg
    16741 ctgccattcc aaaatgcaga atatcctttg gcgccacgcc aagccaggcc aaacccggct
    16801 cgaaatcggg agcgaactca gaaagcaccc aacaccccga gttgcacgcc gccttcgggg
    16861 cgcctacgaa tgctgaagga gacggtgctg cggtccgcgg atcgcacgag ctccgaacgc
    16921 ccactcgcga gacccgcaat cagcaacagc gcgccggtcg cctgcaacat gccgtcaacg
    16981 gccacgagcg cgattgtggt ggcttcttcg tacgcccggt cgaggcagcc tcgcgcctcg
    17041 tcgacggtcg tcgccgcaca cgaaagcttg cgaccggcga gcgcgggcca aggcccgagc
    17101 acaggcaccg cgagccaacc caagctgccc tcgaaatcgc ggttcatgcc aatgcctatg
    17161 gccgttgcgt acccgacccc gagcgccagc gcaccgctca agatcaaccc cgtcgccggc
    17221 cgatactcca cgtgatagcc gcgaggaacg ggtaagccag aacgatacgg caaaaggggc
    17281 ggcaacccca agcgcgcaga tcgcctcgga agcgctcttt gcgcgctggg accggcgtcg
    17341 tcgacatcca agagagctgg atctgcgggc agcgagcccg gaacgacttc acgcttcgga
    17401 cttgccgcgc cagagctgga gcggttcgac tcgacgctcg gagacggggc ggcggccggc
    17461 gcgccgcgtt ggccttccga attcgccgcg cccggacgct ggcgcgggtc gaccttacga
    17521 gttggggcaa ttcgctcgtt ctcaggcgca ggtgagggcg ctggctccgc gctggggaaa
    17581 gcacccgtcg tttgagcgct cgcgagtgcg gaaaaagcca gcgtcgcaaa ggccgtgcag
    17641 aactgcgcgg ctacgcgact cgattggccg gagaacaact cagaccccgc cgcgccagcg
    17701 cttggtctta tcgccccgaa acttggtttt cggcgccttt cacaccgccc ggacatctcc
    17761 gtgaccgtag ccgagcgccc cgtgtctcgc aaggcgcacc cgcccccgcg aactggcagg
    17821 ttttcagatc gattcgaacc gggcatgctc ctcgtgcatg agccctgacc ctacgctgat
    17881 atccaccccg aaaggtccgc gttccgatgc cctggtcttc ttcggcgcta cgggagatct
    17941 cgcgtacaag aagatctttc ctgcgctg
//
LOCUS       DASTKU010000525        20190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_547812, whole genome shotgun sequence.
ACCESSION   DASTKU010000525 DASTKU010000000
VERSION     DASTKU010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 20190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20190
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547812"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..4687
                     /locus_tag="VFQ61_18110"
     CDS             266..4687
                     /locus_tag="VFQ61_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9956432.1"
                     /translation="MTPELPARYEPLKRLGQGGGGEVWAVRDRHGGSVFALKVLAEDS
                     SEPERAALVREAAALSGLEGLGLPRVVSFGRLPKSGRAFLVRELVEGVSLDSLIQGDG
                     PLQPILEALVRAADQLTVLHRAGLLHGDVKPANIIVEADGATTFVDLGLAAPFQEGGT
                     AAEGLSPRYAAPELFEGKPLTVRAEVYALGVALKEAVEQHSREGVLARALLEELSRVA
                     AKATSASPLDRYPSVDEFGSALRHAAGLPQPTETTQHVALWPILGTEGISQQLLERAS
                     SLPNGALMRLSGPAGAGKSALLRRLSWSLGVEGQPVAFFDGQATAQFIASEFEAHPQL
                     SALFVLVDDTDDLPASVCERLLAARAVGARLIVVGGQELGPAQCEVEVPPLDFHSARD
                     LVKRAIPSLGEALQKRVIELAGGRPGPLRSILGSIESEAIASATEIERVLSASPIKLE
                     APEDPLQRAVYYLDRGRYNDAQVALDELPESIAQSSVAASVARARLELCTGESASALA
                     RLAPYAERAESLGAERNELVLYLGRAYIAVGEYAKAIETLAPLIELPGSLALEALSYH
                     GLGLSLLGDHEAARRELSQAVSRAKTLGDARAEAVALASQGMEFQRSDHVDDARRAYE
                     GAIVAAERAGDAGNVAVVRANLAGLLKICGDVAGAIEQFEAALDMSRRSGRRQTARQA
                     LLNLANLDLYLGRLARARERIEALELQRAQLPPVTRAQLLGLWAEFRAKQGELEPAVE
                     KYEECALAYEALGRGVDAAEARLEGVLTAVRLPHPDVAELARRIEAGRAALGQSLSHR
                     PLLLLAQARVATRAADEATARARLGEALAAARESGQREWCWRALEARAALEESGGQPL
                     LARRDREEAVAILEEIGARLPRDLREVYWNDARRSQLRGSVPQAISHAATEYLPFRGN
                     LMSERHAAPAGVTTTSLSDLPQTPLEQRLARILDVNAELAQEADVGLLTARIIDHALE
                     LSRAERGLLILRGEGDSLTIQTSRASGNDVPDPEFSRSIAERVLASREPFVALSAKND
                     ARIAAHASVHQLLLESVACLPVLAPSGRAVGALYLETRLKQGARFERELPILKAFAVQ
                     VAIALENARLLGENRKRADELADSNRNLEQAQERLRELLGDRTEQLRRARRRLKDVRD
                     TLYGHFGYHGLVGTSSAMRRVYALIDRVKDTDVPVLITGESGTGKEMVARAIHATSPR
                     AKAKMLGVNCGAIPENLLESELFGHVRGAFTGADRERKGLFREADGGTILLDEIGETP
                     AKMQAGLLRVLQERKVRPVGGGQEETVDVRLVCATNRDLLALVREGKFREDLFYRIHV
                     VEVKLPALRERLDDLPQLMDHFLGIFAARYKREKKSVSREALRVLSNYEWPGNVRELE
                     NVLLNAWVMSEDDELLPEDFSLPEARAAVRKASEVTEERPVKRAEKGTTSEHHRGERE
                     RILEALKSCNWNRVKAAQLSGIPRRTFYRRLREYGIQ"
     gene            complement(4716..6662)
                     /locus_tag="VFQ61_18115"
     CDS             complement(4716..6662)
                     /locus_tag="VFQ61_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9956433.1"
                     /translation="MAHVELEVVSGAGQGLKLSSDADVIQVGRDASNDLVLEDARIAP
                     RLLRLWSAGLDEVVLELLDGTVHVQLVRNGQTSALSADARVPLRSGDEFDLAAPGASV
                     ARLRVTIEAERDAGQLLALKPLAELVQAAQVATATGHLRPLLEALRSIGSAEDLEQAA
                     GAVAEAALELVPRATHAAVLLRDDSVPAAHAITHGLGLIATRFRDESAEIVAPEEPFP
                     VPRSIPRKVLRERAAVVVANAPAEAMSSESLLGASIHSTMGVPLWKRDEILGVLQVDS
                     RDAPALFGADDVDLLGVLSVNASLAIANASLIRKLSLAEQNLRRENSFLKGRERARST
                     RVDLVGKSRVMQGLLAQLDKVRDTRVSVLIQGETGTGKELIAALLHHRSRRREHLFVA
                     QNCAAFPENLLESELFGHRRGAFTGATEDKKGLFEIADGGTLFLDEIGEMPLALQAKL
                     LRALQEGEIRPLGATSAKQVDVRIVAATNRDLDAEVKAGRFREDLLYRLKVFPIRVPP
                     LRERREDVPLLAQHFLERYTVEFGKNIGGFEPEALHTLSAYDWPGNVRELENEVQRLV
                     IQAEPDDRIGPQLLSPRIRQSEELVLGAAQAKGTLRAMVDQVERQILLETLREHNNNK
                     TATARTLGITREGLHKKLKQLGIG"
     gene            complement(6823..8580)
                     /locus_tag="VFQ61_18120"
     CDS             complement(6823..8580)
                     /locus_tag="VFQ61_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956434.1"
                     /translation="MLRSSRSVASLIGSLVFGTVLPTTVTLGVLVVSGCKDESQPEYW
                     VEKLSEPTWRSRAISRFDQFFEDALTRSNKDLKSQDVQDLINKTVEPLTKAYVEAADQ
                     LDPKSRVTLIKLLSGYKDKRAEPAFKKAFEDFAKSPRTSKDDQDLKWAAIAAGDLRLE
                     SLAGPMVEAFAKFRASTMLGGVVYKDFNAALLDMPSKSWTQPLISKLEAEVRKPQGKA
                     DPSAVGDYMDQQFWQTTAAALLGRIGDPAAVEPLIRVILDPARVDFHQTAMVALIQLG
                     KPSTDAAVKLLKNQDEKLTAYCQRRLKETGWDGKGKPCVQTAAVIIGSVGRPDGAPAM
                     IEVLKTENDDATKAVIARELSKLPASAESIAAFKAAYESLPLSAQIPPGQPALEILTE
                     SAGQVFDASLVEWLLGRAESIKADKSEADEKKSLQLAILTTTIKLAKPDQVPVVKKAV
                     NSYGTKLEKDLLAQAEELLKACGDRAPCYVEALQKRENQERSAQFVGIKAGYMAAEFG
                     DEATRDALVSRLDSINNAALRFVASQVVDRLSPKGSKQSVEKMNAIIEKNAKSPDRDK
                     ALGDAPLKQVMYRLSARGG"
     gene            complement(8828..9985)
                     /locus_tag="VFQ61_18125"
     CDS             complement(8828..9985)
                     /locus_tag="VFQ61_18125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9956435.1"
                     /translation="MNREPLATDADLVVIGAGIMGLATAYHVARLAPRLRICVIEGSY
                     LCSGASGRNGGGVRAQWADELSIRLMRESLESFRTFAREHRINTWFRQGGYLFLARSP
                     ERAAELEHSVQLQNRLGLQTRLLAAEDIHEVVPELATAGVLVASYNPEDAVVFPWPLV
                     WGYAESARARGVQIATFTRAVGLELTRSRVSAVHTARGTIRTSRVVVAAGAHSPIIGR
                     WAGVELPTRPQRHEICASEPLKPFLNPLVADLTDGLYFSQSTRGELVGGISDIGENRT
                     QSSSSEFLVHYAKALVDRCPILGQVRVLRQWAGLYDISADGGPLVGEVDSAPGLYLAS
                     GFMGHGFMMAPVIGQRLAFAITSDSPPPELAAWNIRRFEQGQRFEGGMVIG"
     gene            complement(10095..10919)
                     /locus_tag="VFQ61_18130"
     CDS             complement(10095..10919)
                     /locus_tag="VFQ61_18130"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HET9956436.1"
                     /translation="MNLEELSILVGIAAEASELVRGVYGRHFEVAYKAPEDPVTEADR
                     AANTLICERLREAFPNCPIVAEESPPETFADFRRAPRVFFVDPVDGTREFVRRNGEFV
                     VMIGVLEENSAVASVIQAPALGFTWVGLVGTGAFQIDADGQRTQIRVSEAAELSQARV
                     ACSRSHNTGEVEQVLDGLGAAELVQLGSAGLKGAHVATARADAYVAPHYAGQRWDACA
                     PDALVRAAGGNVSDAFGEPIDYRSDSLYNDRGIVMTNGLLHVPVLSSIDRTRLSRR"
     gene            complement(10916..11860)
                     /locus_tag="VFQ61_18135"
     CDS             complement(10916..11860)
                     /locus_tag="VFQ61_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9956437.1"
                     /translation="MRKRPLGGTGLEVTELALGTWGLCGDAYGPVPEAEQDAVIERAL
                     SMGIRLFDTADTYAEGKMEERLGRRLPNDRSCVVVTKVGTRRDTTPSQKNFTPEFLER
                     SIEASAARLGDGVVRCVLLHNPSPQAYQRNDVGALMSGFKQRGLLHHWGVASGNVEVG
                     RAALECGAEILELAYNIFHARELHQLEPEIAERNVGVLARSVLAHGLLTGSWLPNKEF
                     PSTDHRALRWNTDELRHRIQQLNPIRTLLSQNLPSLRAIALRFGLEHARVSSVVLGPR
                     NGLQLDQLVREAGREPPYIEPERLADLRTRLAVIGINL"
     gene            complement(11905..13962)
                     /locus_tag="VFQ61_18140"
     CDS             complement(11905..13962)
                     /locus_tag="VFQ61_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956438.1"
                     /translation="MDEPSAFPALRGLLAPLERVGRLPRIHLLLRWVLLGVPTACLAC
                     LALQSVLEKNMGLPAAPLDDSYIHLQYARNFAHGLPLRYAPDAPPVAGATSLLWPALL
                     AIFHALGLQGESLIWPAWVLGFVSLGLLAHEAERAAASFVAPKHAVFAAALVLCFGAN
                     TWFAGSAMEVVPLSWLLLRSVRRAADWWERPESAASGHRTELELTTLAWLAPLMRPEG
                     ALGSMAIILVLSLKPPRPRRMAVWAALGVFAPATINWLITGQATTTTMRAKWLPVNPY
                     SSVSSLVEAMWQHIELLFGTLLNGEIWSALFLPKGCWPVWLAAFATLVWRATRGPTRM
                     RAALLLLLALGILIPGTFECPLCNRLRYLWPFFPAWMVGLVGFADLLSRAIERWNSSL
                     GYVTPGLLGAVSGALLGYMPFALQDLGASASAISAQQVALGKWARTALPGDARIGVND
                     TGAIAYFSERSTFDIVGLTTSTEAEHWTAGPGSRFEHYERLGRAALPTHFIVYPEWFA
                     LDELCGEVLNERAVPGATILGGERMVAYRADYSRLGSGETPDLESAQGFRVIDQLDVA
                     DLVSERAHDYALLEGAQRYDDLVRFGTRLDGARKQRSRDAFRLDLAPGGRLVLRLGST
                     SPTWVRVRIGPSTLRAELPASVWHEVVMKLPEDLPAGRNAVSVECGEPFTSMHYFSLV
                     PAK"
     gene            13961..14599
                     /locus_tag="VFQ61_18145"
     CDS             13961..14599
                     /locus_tag="VFQ61_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9956439.1"
                     /translation="MKHHPYAPIAPRLPEAETIDPREILQSGGRPIELEIGPGRGGFI
                     LERLEAAPEARMLGLEIRLKWATLVDRKLGERGLHSRARVFAEDARAAIRRFPDGCLE
                     AVFIHFPDPWWKKRHQKRLVMGDALLAELSRTLRPGGQVFLQTDVEERASFAQALLDA
                     NPHFSPKYASARIEENPFLARSPRERRAIQDGLPIYRLLYERRAAEPAASVP"
     gene            14633..15085
                     /locus_tag="VFQ61_18150"
     CDS             14633..15085
                     /locus_tag="VFQ61_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956440.1"
                     /translation="MRARFFVSVLACVALQSCVTYREQLNRGQRLYEENQYERALALL
                     RNLDGDVDSLSSVEQSRYYYLRGMTDYRLGYRAEARHFLATAQAADALQPGGLIPEWQ
                     ERMRAALAELNHDVYGGDGPSPDVVYPQPTSTTPGTSPTSSESSVSTP"
     gene            complement(16154..16333)
                     /locus_tag="VFQ61_18155"
     CDS             complement(16154..16333)
                     /locus_tag="VFQ61_18155"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HET9956441.1"
                     /translation="MDTKVIDRVRKLELLHRNLDAEVTRLGRRAYLTPIEQREFMELK
                     KQKLLAKDQLAALRR"
     gene            17612..18727
                     /locus_tag="VFQ61_18160"
     CDS             17612..18727
                     /locus_tag="VFQ61_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956442.1"
                     /translation="MPKTIVTRTSATSIPELYEEAWSASRAGEHARAAELFEKVYSLE
                     PEGPLADDALFQSAAESDAAALLEAAASKYRELWERYRESPLSGTAAVRALRILVHLE
                     ALDRAEPLARQLLDGNVTLLPGERVVVRGALALSALARDAETDARRHIEEGRNIVDAQ
                     QLDSADRVSPDLAPLYFALGELRRRLGDRIVFEPMPVNFPAALEQRCQHLLDAQSAYL
                     DAIRARDAHWSAMAGFRVGELYQTLHRDLMKVTPPASADTEAKRQLFEGAMRVRYSIL
                     LTKARAMLEHTLAMAERTAEHSRWVDRAREASQAIQRAVEEENAALDRLPYSRETLAA
                     ALADLEKRSAEKAATNKAGPVKPGSANPGKSTPSMRP"
     gene            19274..19346
                     /locus_tag="VFQ61_18165"
     tRNA            19274..19346
                     /locus_tag="VFQ61_18165"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19307..19309,aa:Thr,seq:tgt)
     gene            19450..19532
                     /locus_tag="VFQ61_18170"
     tRNA            19450..19532
                     /locus_tag="VFQ61_18170"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19484..19486,aa:Tyr,seq:gta)
     gene            19655..19727
                     /locus_tag="VFQ61_18175"
     tRNA            19655..19727
                     /locus_tag="VFQ61_18175"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19688..19690,aa:Gly,seq:tcc)
     gene            19861..19933
                     /locus_tag="VFQ61_18180"
     tRNA            19861..19933
                     /locus_tag="VFQ61_18180"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19894..19896,aa:Thr,seq:ggt)
     gene            19996..>20190
                     /locus_tag="VFQ61_18185"
     CDS             19996..>20190
                     /locus_tag="VFQ61_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9956443.1"
                     /translation="MAKEKFVRTKPHVNVVTIGHIDHGKTTLTAALVKVQSKKNMAKE
                     IKYSDIAKGGIVRDETKTVTI"
BASE COUNT         3516 a         6468 c         6469 g         3737 t
ORIGIN      
        1 gagccaagtt gggcttccgg cggctccaag ccaatcatct tcccgtggct cgccgtccag
       61 accgggctgc gttacaagcc gcacaagaag ttcgtggggc gcttggattt gggctttggt
      121 ctgagcggtc cgttcatcgg aattggcgct gattacggta tttgatgcgg gcgggcgtgc
      181 gggtaacggg tttttgtgcg ggcagcaggg ttgattgctt gggcgggcgc cgaggcagtc
      241 tttcacgcag ggtgaggttt ggagcatgac gccagagctt ccggcgcggt acgagccgct
      301 gaaacgtttg ggccaaggcg gcgggggtga ggtttgggcg gtgcgcgatc gccatggcgg
      361 tagcgtgttc gcgctcaaag tgctggccga agactcgagc gagccggagc gcgcagcgct
      421 agtgcgggag gccgccgcgc tctccggtct ggaagggctg gggctcccac gtgtggtgag
      481 cttcggtcgg ctgccgaaga gcgggcgcgc gttcttggtg cgcgagctgg tggagggcgt
      541 gagcttagac agcttgatcc aaggcgatgg gcccttgcag ccgatcttgg aggcgctggt
      601 gcgcgcggcc gatcagctca cggtcttgca tcgcgcgggg ctcctacatg gtgacgtgaa
      661 gccggcgaac atcatcgtcg aggcggatgg agccactacg ttcgtagact tgggcttggc
      721 cgcgccgttt caagaaggcg gcacggccgc ggagggcttg agcccgcgtt acgccgctcc
      781 cgagctgttc gaaggcaagc cgctcacggt tcgtgccgaa gtgtacgcgc tcggggtcgc
      841 gctcaaagag gccgtggagc agcactcgcg tgagggtgtg ctggctcgag cgctgctcga
      901 ggagctatcg cgcgtggcgg ccaaggccac ctccgcttcg cccctcgacc gttatccttc
      961 ggtggacgag ttcgggagcg cgttgcgtca cgcggcgggg ttgccgcagc ctaccgagac
     1021 cactcagcac gtcgcgctct ggcccatcct gggtacggag ggcatctcac aacaactctt
     1081 ggagcgtgcg agtagcctgc cgaacggcgc tttgatgcgg ctatctgggc cagccggagc
     1141 ggggaagagt gcgctcctac ggcggctctc gtggtcgctc ggggtggaag ggcagccggt
     1201 cgcgttcttt gacggccagg ctaccgcgca gttcatcgcg agcgagttcg aggcgcaccc
     1261 acagctctcg gcgttgttcg tgttagtcga tgacaccgac gacctgccgg cttcggtgtg
     1321 cgagcgcttg ctcgcggccc gcgccgtggg cgcgcgtttg atcgtcgtgg ggggccagga
     1381 gcttggcccg gctcaatgcg aagtggaggt cccgccgctc gatttccatt cggcgcggga
     1441 tctcgtgaag cgcgccattc cgtcgctcgg cgaagcgctc caaaagcggg tgatcgagct
     1501 cgcggggggg cggccaggac cgctgcggag catcctgggc tcgatcgaga gtgaagcgat
     1561 cgcctctgca accgagattg agcgcgtctt gagcgcgagc cccatcaagc tggaggcgcc
     1621 cgaagacccg ttgcagcggg cggtctacta cctggaccga gggcgctaca acgacgcgca
     1681 ggttgcgctc gatgagctgc ccgagagcat cgcgcagagc tccgttgccg cctcggtggc
     1741 gcgagcccgt ctcgagctct gtacggggga gtcggcatcg gcgttagcgc gcttggcacc
     1801 ctacgcggaa cgcgccgaga gcctgggagc cgagcggaac gagctggtcc tgtacttggg
     1861 gcgggcgtat atcgcggtcg gggagtacgc caaagccatc gagactttgg cgcctttgat
     1921 cgagctgccg ggctcgttgg cgctcgaggc cctgtcctat cacggcctcg ggctgtcgct
     1981 tttgggagat cacgaggccg cgcggcgtga gttgtcgcag gccgtgagcc gcgcgaagac
     2041 cctgggagac gcgcgcgcgg aagccgtggc cttggcgtcc caaggcatgg agttccaacg
     2101 ctcggatcac gtcgatgacg cgcgccgtgc ctacgaagga gcgatcgtcg ccgccgaacg
     2161 cgcgggggac gccggcaacg tcgcggtggt gcgggcaaac ctcgcgggcc tgctgaagat
     2221 ctgcggcgac gtcgcgggcg ccatcgaaca gttcgaagct gcgctcgaca tgagccgtcg
     2281 ctcgggtcgg cgacagaccg cgcgacaagc tttgttgaat ctcgcgaact tggacctcta
     2341 cttggggcgt ctggcgcgtg cgcgggagcg gatcgaggca ttggagctgc aacgggcgca
     2401 gctaccgccg gtcacccgcg cccagttgct cgggctctgg gcagagttcc gagccaagca
     2461 gggcgagctc gagccagcgg tcgagaagta cgaggagtgc gcgttggctt acgaggccct
     2521 gggtcgcggc gtggatgcgg ccgaagcgcg gctcgaaggt gtgctcaccg ccgtgcggct
     2581 gccccacccc gacgtagcgg agctggcgcg acgcatcgaa gccggacgcg cggccctcgg
     2641 ccagtctctg tcgcatcgac ccctattgct cctggctcaa gcgcgggtcg cgacgcgcgc
     2701 cgcagacgag gccacggcgc gcgcgcgttt gggggaagct ctcgccgccg cacgcgagtc
     2761 gggtcagcgt gagtggtgtt ggcgtgcgct cgaagcacgc gccgccctcg aagaaagcgg
     2821 cgggcagcct ttgttggcgc gccgcgatcg agaggaagcg gtcgcgatcc tggaagaaat
     2881 cggagcgcgg ctgccgcgag atctacggga ggtttactgg aacgacgcgc gccggtccca
     2941 gctgcggggc tcggtgccac aagccatctc gcacgcggcg accgagtacc tgccgtttcg
     3001 cggcaacttg atgtccgagc gccacgccgc gccggcggga gtcacgacga cgagcctttc
     3061 ggacttgccg cagactccgc tcgagcagcg tctggcgcgg atcttggacg tgaacgcgga
     3121 gctcgcccag gaggcggacg tcgggctctt gacggctcgc atcatcgacc atgccctcga
     3181 actctcgcgc gccgagcgtg ggctcttgat cctgcggggc gaaggggact ctctcaccat
     3241 ccagacctcg cgcgcgagcg gtaacgacgt gcccgatccg gagttctccc gctcgattgc
     3301 ggagcgagtg ctcgcgtcgc gtgagccgtt cgtcgcgctc agcgccaaga acgatgcgcg
     3361 catcgccgcc cacgcttcgg tgcaccagct gctgctcgag tcggtggcgt gcttgccggt
     3421 gcttgcgccg agcggccgcg ccgtgggcgc gctctatctc gagacccgtt tgaagcaggg
     3481 ggcgcgtttc gaacgcgagc tccccatttt gaaggcgttc gcggtgcagg tcgcgatcgc
     3541 cttggagaac gcgcggctac tcggggaaaa tcgcaagcgc gcggacgaac tcgcggactc
     3601 gaaccgcaac ctcgagcagg cacaagagcg gctgcgtgag ctactcggcg accgtaccga
     3661 gcagttgcgc cgggcgcgcc gccggctcaa agacgtgcgc gacacgctgt acggccactt
     3721 cggctatcac ggcttggtcg gcacgagctc tgccatgcgc cgggtgtacg cgttgattga
     3781 ccgcgtgaag gacaccgacg tgcccgtgct catcaccggt gagagcggca ccggcaagga
     3841 aatggtggca cgcgccatcc acgccacttc accccgggcg aaggccaaaa tgctcggcgt
     3901 gaactgcggc gcgatcccag agaacctgct cgagagtgag ctgttcgggc acgtgcgtgg
     3961 cgccttcacg ggcgccgatc gcgaacgcaa gggtctgttc cgcgaggccg atggcggcac
     4021 gatcctgctc gacgagattg gagagacccc cgccaaaatg caggccgggt tgttgcgcgt
     4081 gctgcaagag cgcaaggtgc gccccgtggg cggaggccag gaagagaccg tcgacgtacg
     4141 cttggtgtgc gcgacgaacc gtgacctgtt ggcgctggtg cgggagggca agttccgcga
     4201 agacttgttc tatcgtatcc acgtggtcga ggtgaagctg ccggcgttgc gggagcgcct
     4261 agatgacttg ccgcagctca tggatcactt cctcgggatc ttcgcggctc gctacaagcg
     4321 cgagaagaag tcggtctcgc gtgaggcgct gcgcgtgctc tccaactatg aatggcccgg
     4381 caacgtgcgg gagctcgaga acgtgctcct gaacgcatgg gtcatgagcg aggatgacga
     4441 gctgctcccc gaagacttca gcttgcccga ggcccgcgcc gctgtccgca aggcttccga
     4501 ggtcaccgaa gagcgccccg tcaaacgcgc cgaaaagggc acgacttcgg agcatcaccg
     4561 cggcgaacgc gagcggatcc tggaagccct gaagtcgtgc aattggaacc gggtcaaagc
     4621 ggctcaactg agcggaattc cgagacgcac attctaccgc cgcctgcgtg agtacggcat
     4681 tcaatgaccc acgcggggtg agcgccgcaa gcgcgctagc cgatccctag ttgctttagc
     4741 ttcttgtgca aaccctcgcg ggtgatgccc agggtgcgcg ccgtagccgt cttgttgttg
     4801 ttgtgctcgc gtagcgtctc cagcaaaatc tgacgttcga cttggtccac catggcccgc
     4861 agcgtgcctt tggcttgggc ggccccgagc accagctcct cggattggcg aatgcggggg
     4921 ctcagaagtt gaggcccgat gcgatcatcg ggctcggcct ggattaccag gcgttgcacc
     4981 tcgttctcga gctcgcgcac gttgccaggc caatcgtacg cggacagggt atgaagcgct
     5041 tccggctcga agccaccgat gttcttgccg aactcgaccg tgtaacgctc caagaagtgc
     5101 tgcgcgagca ggggcacgtc ttcacggcgc tcgcgcaggg ggggcacccg gatgggaaat
     5161 accttcaggc gatacagaag atcctctcgg aagcgccccg ccttcacctc ggcatccagg
     5221 tcgcgattgg tcgcggctac gatgcgcacg tccacctgtt tagcgctcgt cgccccgagc
     5281 gggcgaatct ctccctcttg cagcgcgcgc agcaatttgg cctggagcgc gagtggcatc
     5341 tcgccgatct cgtccaggaa caacgtgccc ccgtccgcaa tctcgaacaa gcccttcttg
     5401 tcctccgtgg cgccggtgaa agcgccgcgt cgatgtccga agagctcgct ctcgagcaag
     5461 ttctccggga aggcggcgca attttgggcc acgaacaggt gctcccgccg ccggctccgg
     5521 tgatgcagca acgcggcgat cagctctttg cccgtgcctg tctcgccctg aatcagcacg
     5581 ctgacgcgcg tatcgcgcac tttgtcgagc tgggcgagca gcccttgcat cacgcggctc
     5641 ttgccgacca aatccacgcg ggtcgagcgc gctcgctcac gccccttgag aaagctgttc
     5701 tcgcggcgca ggttctgctc ggcgagtgac agcttgcgga tcaagctcgc gttcgcgatc
     5761 gccagcgagg cgttcacgct gaggacaccc aagagatcta cgtcgtccgc gccgaacagg
     5821 gccggggcgt cacggctgtc gacctgcagc acgcccaata tctcgtcgcg tttccagagg
     5881 ggtaccccca tcgtgctgtg aatactcgcg ccgagcagcg actcggagct catggcttca
     5941 gccggggcgt tggcgaccac caccgccgcg cgttcgcgca acaccttgcg cgggatgctg
     6001 cgcggcaccg ggaaaggctc ctcgggcgcc acgatttcgg ctgattcatc gcggaaacgc
     6061 gtggcgatca gaccgagccc atgggtgatg gcgtgggccg cgggcacgct atcgtcgcgc
     6121 agcaacaccg cggcatgggt cgcgcggggg accagctcga gcgctgcttc ggcgacggcc
     6181 cccgcggcct gctcgagatc ttcggcgctt ccgatgctgc gcaacgcctc caagagaggc
     6241 cgtaaatgcc cggtcgcggt ggcgacctgg gccgcctgca ccaactccgc cagcggcttg
     6301 agggccagca gctgccccgc gtcgcgctcc gcctcgatcg tgacgcgcag ccgagcgacg
     6361 ctcgcgcccg gcgcggcgag atcgaactcg tcaccgcttc gcagtggcac gcgggcgtcc
     6421 gcgctcaagg cgctagtttg gccattgcga acgagctgca catggacggt cccatcgagc
     6481 aattccagga cgacttcgtc gagaccagcc gaccaaagcc gcagcaaccg cggcgcgatt
     6541 cgggcgtctt ccagaacgag atcattgctc gcgtcgcgac cgacctgaat gacatcggca
     6601 tcactcgaaa gcttaagacc ctggcccgct cccgacacca cttccaactc gacatgcgcc
     6661 atgcgacagg gtcaccttaa ctcaaactgc cgcgattgag gggagggggc cgccgctcgc
     6721 cgagaaaggc gccgcttctt cgcgtctgga tgaaccccac aaacggcaaa ggccgcgtcg
     6781 ctgttaccag tgacgcggcc ctaattcggt tcgtacgctg cctcaaccgc cgcgcgccga
     6841 caggcggtac atcacctgct tcagcggcgc gtcgcccagc gccttgtcgc gatcgggtga
     6901 tttggcgttc ttctcgatga tcgcgttcat cttttctacg gactgcttcg atcccttcgg
     6961 tgagagccgg tccacgacct gagacgccac gaagcgcagg gccgcgttgt tgatgctgtc
     7021 caagcggctg acgagcgcgt ctcgggtcgc ttcgtctccg aactcggccg ccatgtagcc
     7081 ggctttgatg ccgacaaact gcgcggagcg ctcctgattc tcacgcttct gtagcgcctc
     7141 cacgtaacac ggggcccgat cgccacaggc cttcaggagc tcttccgcct gcgccagcag
     7201 atccttctcc aacttcgtgc cgtagctgtt tacggccttc ttcacgaccg gcacctgatc
     7261 gggcttggcg agcttgatcg tcgtggtgag gatcgcgagc tgcagcgact tcttctcgtc
     7321 cgcctcgctc ttgtcggctt tgatgctctc cgcgcgtccg agcagccact cgacgaggct
     7381 cgcatcgaag acctgccccg ccgactcggt caagatctcg agtgccggtt ggccaggcgg
     7441 gatttgcgcg gaaagaggca ggctttcata ggcagccttg aaggcggcga tcgactccgc
     7501 cgatgcgggc agcttcgaga gttcccgagc gatcacggcc ttggtggcgt cgtcgttctc
     7561 ggtcttgagc acctcgatca tggcgggcgc gccgtcaggg cgacccactg agccgataat
     7621 tacggctgcg gtctgcacgc agggctttcc cttgccgtcc cagcccgtct ccttcagtcg
     7681 gcgctgacaa taggcggtca gcttctcgtc ttgattcttc agcagcttga ccgcggcgtc
     7741 ggtagacggc ttgccgagtt gaatcaaagc caccatggcc gtttgatgga aatcgacgcg
     7801 agccgggtcc aagatcacgc ggatcagcgg ctccaccgcg gcgggatcgc cgatgcgccc
     7861 cagcaacgcg gcagccgtcg tctgccagaa ctgctggtcc atgtagtcgc cgaccgcaga
     7921 cggatcggct ttgccttgcg gcttcctgac ttcggcttcg agcttcgaaa tcaagggctg
     7981 ggtccaagac ttcgagggca tgtcgagtaa ggccgcgttg aagtctttgt agaccacgcc
     8041 gcccaacatc gtgctggcgc ggaactttgc gaaggcctcg accatcgggc ccgccaacga
     8101 ctcgagcctc agatcgccgg ctgcgatagc ggcccacttg aggtcctgat cgtccttgct
     8161 ggtgcgcggg gacttcgcga aatcctcgaa ggctttcttg aaggcgggct cagcgcgctt
     8221 gtccttgtaa cccgagagca acttgatcaa cgtcacgcga ctcttcggat cgagctgatc
     8281 cgcggcttct acgtaggcct tggtgagcgg ctcgacggtc ttgttgatca ggtcctgaac
     8341 gtcctgggac ttgaggtcct tgttggagcg ggtgagcgcg tcctcgaaga actggtcgaa
     8401 acggctgatt gctcgcgatc gccaagtagg ctcggagagt ttttccaccc agtactcggg
     8461 ctgactctcg tctttgcacc ccgacaccac gagcacgccg agtgtaacgg ttgtcggcag
     8521 aacggtgccg aacacgagcg agccgattag cgatgcgacg cttcgcgaag aacgcagcat
     8581 tttttggatc tcccaggcag tgacgacgtg ttgaggtgag gggaaacacc gggagcgagg
     8641 ccaagcctcg agcgaagccg gtttcccgcc ccagcggaac gtaagctggg cgcgttcttg
     8701 ctgtcaatta gcgtgctcga agcaactgag gagcgtcgca tcgctaatgc attgcgtcag
     8761 ctgcagtgaa tagcggagcc cacatcatct cgaaggcgac agttcgcggc tgcctcggaa
     8821 tccaggctca gccgattacc atgccgccct cgaatcgctg gccctgctcg aagcggcgaa
     8881 tgttccaagc cgcgagctcg ggaggaggtg agtcgctcgt aatggcaaac gccaggcgtt
     8941 gcccaatgac gggcgccatc atgaagccat ggcccatgaa gccactggcc agatacaagc
     9001 caggtgcgga gtcgacttcg cccacgagcg gtccgccatc ggctgagatg tcgtacagcc
     9061 ccgcccattg ccggagcacg cgcacctggc cgaggatggg gcagcgatcg acgagcgcct
     9121 tggcgtagtg aacgaggaac tcactcgacg agctttgagt gcggttttcc cctatgtcgc
     9181 tgatgccgcc cactagctcg cctcgcgtag attgagagaa atataggccg tcagtcaaat
     9241 ccgcgacgag cgggttgagg aagggcttca gcggctcgct cgcgcagatc tcgtggcgct
     9301 gcggccgagt gggcagttcg actccggccc agcgcccgat aattgggcta tgtgccccgg
     9361 ccgcgacgac gacacgcgat gttcgaatcg tgcccctcgc ggtgtgcacg gcgctcacac
     9421 gcgatcgcgt gagctcgagg cccaccgcgc gcgtgaaggt cgcaatctgc acgcctcgcg
     9481 cgcgggcgct ctcggcgtag ccccagacca gcggccaggg aaacaccacc gcatcttcgg
     9541 ggttgtagct cgcgaccaat acccccgcgg tcgcgagctc cggtacgacc tcgtgaatgt
     9601 cctcggccgc cagcaagcgc gtctggagcc caagccggtt ctgcagttgc acgctgtgtt
     9661 cgagctccgc cgctcgctcg ggcgagcgcg ccaagaacag gtagccaccc tgccggaacc
     9721 acgtattgat gcggtgctcg cgcgcaaaag tccggaacga ctctaggctc tcgcgcatca
     9781 gccgaatcga gagttcgtcc gcccattgag cgcgcacacc accaccgttg cgtccgctcg
     9841 ccccgctgca cagataggag ccttcgatca cgcagatacg gagccgcggc gcgagccggg
     9901 caacgtggta cgcggtcgcg agacccatga tcccagcgcc gatcacgacc agatccgcgt
     9961 cggtagctag cggctctcga ttcatgaggt ccggactcgc catgggtgac gtcggttctg
    10021 cgtgaaaggg ttctcgaacg ggctcggaga gcggctccag cgcatccgct cagcggggcg
    10081 acgatcgatt gatctcagcg acgcgagagt cgcgtgcggt cgatgctgga gagcaccggc
    10141 acgtggagca gaccgttggt cattacgatg ccgcggtcgt tgtacaagct gtcgctgcga
    10201 taatcgatcg gttccccgaa cgcgtccgag acgttgccgc ccgccgcgcg gaccagcgca
    10261 tccggcgcgc acgcatccca acgttgacca gcatagtggg gcgcgacata agcgtcggcc
    10321 cgggcggtcg cgacgtgcgc tcccttgagc ccggcgctgc cgagctgcac cagctcggct
    10381 gcccctaaac cgtccagcac ctgctcgact tcgcccgtgt tgtgcgaacg cgagcacgcg
    10441 actcgcgcct gactcagctc ggccgcctcg gatacgcgaa tctgtgtgcg ttgcccatcg
    10501 gcatcgattt ggaatgcacc ggtacccacc agcccgaccc aagtgaaccc caaagcaggg
    10561 gcttggatca cgctcgccac cgccgagttc tcctcgagca cgccgatcat caccacgaac
    10621 tcaccgttgc gacgtacgaa ctcacgcgtc ccatccacgg ggtctacgaa gaacacgcgc
    10681 ggcgcgcgtc ggaaatccgc aaacgtttcg ggcgggctct cctccgccac gatcgggcaa
    10741 ttcggaaacg cctccctcaa acgctcgcaa atcagcgtgt tcgccgcgcg atccgcctcc
    10801 gtgaccggat cttcgggtgc cttgtaagcg acctcgaaat gacgcccata cacaccacgc
    10861 acgagctccg aggcttccgc cgcaatcccg accagaatcg acaactcttc gaggttcaca
    10921 ggtttatccc aatcacggct aagcgggtgc ggagatccgc gagccgctcc ggttcgatgt
    10981 aaggtggctc acgcccggct tcgcggacca gctgatccag ctgcagccca ttgcgcggcc
    11041 cgagcacgac ggaagagacg cgcgcgtgct cgagaccgaa gcgcagcgcg atcgcccgca
    11101 gcgaaggcag gttttgtgag agcaacgtgc ggatcgggtt cagctgctga atgcggtgcc
    11161 gcagctcgtc cgtgttccag cgcagggccc ggtgatcggt gctcgggaac tccttgttcg
    11221 gcagccatga gcctgtcagt aagccgtgcg ccaacaccga gcgagccaac acgccgacgt
    11281 tccgctctgc aatttcgggc tcgagctggt ggagctcgcg tgcatgaaag atgttgtaag
    11341 cgagctccaa aatctcggcg ccacactcca gcgccgcgcg cccgacttcg acgttcccgc
    11401 tcgcgactcc ccaatgatgc aagaggcccc gctgcttgaa gcccgacatg agcgccccga
    11461 cgtcgttgcg ttgatacgct tgaggggagg ggttgtgcag cagtacgcag cgcacgactc
    11521 catcgccgag gcgggccgcc gacgcttcga tggagcgttc taggaactcc ggcgtgaagt
    11581 tcttctgcga cggcgtcgtg tcgcggcgag tcccgacctt cgtcaccacc acgcagctgc
    11641 gatcattggg caagcgccgc ccgaggcgct cctccatctt gccctcggcg tacgtatctg
    11701 ccgtatcgaa caagcgaata cccatcgaca gtgcccgctc gatcacggcg tcctgctcgg
    11761 cttcgggaac gggaccatag gcgtccccgc acaggcccca agttcccaac gcgagttcgg
    11821 taacttctag tccagtgccg ccgaggggcc gtttacgcat ggcccgggta tagcaccatc
    11881 cgctgcgctt cagacgtctg cgcgctactt tgcagggact agcgaaaagt agtgcatgct
    11941 ggtgaatggc tcgccgcact cgacgctgac cgcgttgcga cccgcgggca ggtcctccgg
    12001 cagtttcatg actacttcgt gccacaccga agccgggagt tcggctcgca gcgtgcttgg
    12061 accgatccgg actcgaaccc aggtcggcga agtgctgcca aggcgcagaa cgagccgacc
    12121 tcccggcgca agatcgagcc ggaatgcatc gcggctccgt tgcttgcgcg caccgtccag
    12181 tcgcgtaccg aagcgcacta gatcgtcgta acgttgtgcg ccttcgagca aggcatagtc
    12241 gtgcgcgcgt tcactcacca agtccgcgac gtccagctgg tcaatcactc gaaaaccttg
    12301 cgcggactcc aggtcgggcg tctcgccgga gcccagccgc gagtagtcgg cgcggtaagc
    12361 gaccatgcgt tcgccgccca aaatcgttgc gccgggcacc gcgcgttcgt tcaacacctc
    12421 accacacagc tcgtcgagcg cgaaccactc cggatagacg atgaagtgcg ttggtagcgc
    12481 ggcgcgcccc aatctctcgt aatgctcgaa gcgtgagcca gggccagccg tccagtgctc
    12541 ggcctccgtg gaagtggtga ggccgacaat gtcgaaggta gaacgctccg agaagtaagc
    12601 gatcgcaccg gtatcattca caccgatccg tgcgtcgccg ggcagcgccg ttcgagccca
    12661 cttgccgagc gcgacttgtt gcgccgaaat cgcactcgcg ctcgcgccca aatcctgcag
    12721 cgcgaacggc atgtagccga gcaatgctcc cgagacggcg cccagtaagc cgggagtcac
    12781 gtagccaagc gagctattcc aacgctcgat cgctcggctc agcaagtccg cgaaaccgac
    12841 cagtcccacc atccaagccg ggaagaacgg ccacagatag cgcaggcggt tgcacagcgg
    12901 gcactcgaag gtgccgggaa tcaaaatccc gagcgcgagc aacagcagca gcgcggctcg
    12961 catgcgtgtc gggccgcgag tggccctcca aaccagggtg gcaaaggccg cgagccacac
    13021 cggccaacaa cccttgggta agaacagcgc gctccagatc tcgccgttca ggagtgtacc
    13081 gaagagcagc tcgatgtgct gccacatcgc ttcaaccagc gagctcacgc tggagtacgg
    13141 attcacgggc aaccacttgg ctcgcatcgt ggtcgtcgtg gcttgcccgg tgatcagcca
    13201 attgatggtg gcgggcgcaa acaccccgag cgccgcccaa acggccatgc gccgaggcct
    13261 tgggggcttc aacgacaaga ccaggatgat ggccatgctc cccagcgccc cctccgggcg
    13321 catcaagggt gctagccagg cgagcgtagt gagctccagc tccgtacgat ggcctgaggc
    13381 cgcgctctcg ggccgttccc accaatcggc ggcgcgccgg acgctgcgca gcaacaacca
    13441 gctcaggggc accacttcca tggcgctgcc ggcaaaccag gtgttggcgc caaaacacag
    13501 caccaacgcg gcggcaaaca ccgcgtgttt gggcgctacg aagctcgctg ccgcgcgttc
    13561 cgcctcgtgc gcgagcaagc ccaagctcac gaaacccaga acccatgccg gccatatcaa
    13621 gctctcgccc tgcaagccga gtgcgtgaaa aatcgccaac aatgccggcc agagcaggct
    13681 cgttgctccc gctaccggag gtgcgtccgg ggcgtaccga agcggcagcc cgtgcgcgaa
    13741 gtttctcgcg tactgcaggt ggatgtagct gtcgtccagg ggcgccgccg gcagccccat
    13801 gttcttttcc aagacactct ggagcgctag acaagccaag catgcagtgg ggacgcccag
    13861 tagaacccag cgcaagagca ggtgaatgcg cgggagtcgc ccaactcgct cgagtggtgc
    13921 tagtagcccg cgcagcgccg gaaacgcact aggctcgtcc atgaagcacc acccctacgc
    13981 ccccattgcc ccgcggttgc ccgaggccga gacaattgac ccccgcgaga tcctccaaag
    14041 cggcggccgc cctatcgagc tcgagatcgg cccgggacgc ggcggcttca ttctggaacg
    14101 cctcgaagct gcacccgaag cacgcatgct gggcctcgag attcgcctga agtgggccac
    14161 gctcgtggac cggaagctgg gtgagcgcgg gttgcattcg cgcgctcgcg tcttcgcgga
    14221 ggacgcgcgc gcggccattc gccgcttccc ggacggctgc ttggaagcgg tgttcatcca
    14281 cttcccggat ccctggtgga agaagcggca tcagaagagg ctcgtaatgg gggacgcact
    14341 gctggctgag ctttctcgaa cgttacgtcc cggcgggcaa gtcttcctgc aaacagacgt
    14401 cgaggaacgc gccagctttg cccaagctct gctcgacgcg aaccctcatt tcagtcccaa
    14461 atacgcaagc gctcgcatcg aagagaaccc gttcttggcc cgcagcccga gagagcgccg
    14521 cgcgatccag gacgggctcc cgatctatcg gctgctttac gagcgtcgcg ccgcggagcc
    14581 tgccgccagc gtgccttgat ctcgccgagc cccccacgtt agcgtagccg ccatgcgcgc
    14641 tcggttcttt gtctccgtct tggcgtgcgt tgcgcttcag tcgtgtgtca cctaccgcga
    14701 acagttgaac cgcggtcagc gcctatacga ggagaaccag tacgagcgtg cgttggccct
    14761 gctgcgaaat ctggatgggg acgtcgactc tctctcgagc gtcgagcaga gtcgctatta
    14821 ctacttgaga ggcatgacgg actaccgtct cggctatcgc gcggaggctc gccacttcct
    14881 cgcgactgct caagcggcgg atgcccttca gccgggcggg ttgatccccg agtggcagga
    14941 aagaatgcgc gctgcactcg cggagctcaa tcacgacgtg tacggcggcg atggccccag
    15001 cccggacgtc gtgtatcctc agcccacgag cacgacgccg gggacctcac cgacgtcttc
    15061 ggagtcctcg gtctccacgc cctgacgtct caaggcgggt gaccacggca tccgaggcgt
    15121 gccgggctcg acgagcaacc acgccagagc agcgcgcacg agccgcgacg cacttgtttc
    15181 acggcgatcg ttcacacgaa ggtgcttcag cttcgctggg ccgcttcgga aacgaggcgc
    15241 gaaccacgtg aacgggtatg cgaggggtga ccgcaaaact gagcgagctc agataggctc
    15301 aaaggaaacg ggcaccgctg gagctcccag ggaggactcc tttcgcggaa tcatctaggg
    15361 catacccggc tacacgcaag gagccccagc ggtgcgctct acaggttcgc tcatgctgga
    15421 ttacgaggga aatctggctt tgccagaggc ttcgagagcc acgacgagtt gccgtgattc
    15481 gattcggcac cttcgtgact tattccctct gcctattgtt gttcgctgcc ctcgcgggca
    15541 gtagatggct tttggttttt ctacagcttt gattggtacg gggtctttag tgacgtttgc
    15601 ctctactggt tgcccttacg ggtcttcttt ttcttcggag gtgagcttcg tgaattgagc
    15661 tttgtgaata ctcagagctt ccggatattc gacctcgagc ggctcgacgc gctttggggg
    15721 ggtgagggtt tcggactctc tacatttccg agtgtttggt ttctgtgtgc tcttgttctg
    15781 gcgagcgttc tttcagcttc aaccgctgac cagtgttaga ccgtcggttt tgacttagtc
    15841 gctttgggtc tacgtcggtt gctttggtct acgtcgcttg gtctacctcg tggatgatgg
    15901 ggactctgtt gattgggctt cgaaggggta actggccctc cccgtgatct attccctgcg
    15961 aattcagctc cctgctgatt cagcagtggg ttggactctc aacggttgtg tcttgctgat
    16021 cggggcctcc ttcctcggcg gaatcccgtg cttcggctct cttcgacgag cggcttcact
    16081 ccgtttccga attggtgttg tttccgcatc ggtgttccgc atcggtgttc cgcaccggtc
    16141 cagcctccgt gcgctaacgc cggagagctg caagttggtc tttggcgagc agcttctgct
    16201 tcttgagctc catgaactcg cgctgttcga tgggggtgag gtaagcgcgc ctccccaagc
    16261 gcgtcacctc tgcgtccagg tttcgatgca gtaactcgag ttttcgaacg cgatcgatca
    16321 ctttcgtatc catgctcgcc ctgtcctcct aattttctta tttcaaactt ggcgcgacca
    16381 gaatggcgcg aggattgtat ccactggtct gctgcactgg tctgctgcac atcggcattc
    16441 tgccggtccg ttgcatcctg ctttgcttct ggctctggtc tgacgtgcgt tgccctcttg
    16501 cccaacgtgt gaggcgcgaa agaatttggg gcacgaacta gcgcggacaa gggcctatgt
    16561 ggctcgatgc ttgccgggtc tcccctcgac aaatacggca gccccgatcg ctgtgacggg
    16621 ctccaacggc caattcatgg ctctgatgtg tgccggtagc ttcatagtgg catgacgcga
    16681 cacccactcg atgaagtaat tcagaagcac tcgtttgcgt actgcgcccg cggcgccgtc
    16741 gctactggcg atagattaga actttgaatc cgagagtcaa tacgtgaatt cggccgatcg
    16801 ccccccgccg tgcgcttttt catgagtcag tgattgttgc gcaggattgt ttcgataagc
    16861 ccaggtggct gtcgataact gcccctatcg agcgccccgc actggcacgt tcgggaggct
    16921 cgatgagcgg ctgccacgtg cggccaacga tcacgtctcg acgcctatcg cgtcgcgcgg
    16981 gacgccctcg ggtcaccggc gtttcatgca gaatttcgcg tcccctgctg gttgaaggga
    17041 gcgccggcag ggcacttccc aagggctccg ggcggttcga atcgctcgag cgagcgtgcg
    17101 aagtgctggg cgtagatcat gccctgaacg gaagtcatgt ttcgatccag cgtcggcgcg
    17161 gatttcgcaa tccgacgcgc tctcgagtcg cgcagcctgc ctgtgcaacg agattgccta
    17221 tttacgtccg acattcgaat tttgccgcgc tgcgctgttc tcggagccgg gcggcgaggc
    17281 gtacgcgagg tcgcggaaca acaagctcgt tgcagcgggt atgccgcgtt gttcagcgtg
    17341 ttagcggtcc tggtaccggt tccgtggctt gggcgcacgc ttgcagaggc ttgcctcgtg
    17401 catttttgcc gacgttagag cggcttcgtg ggcctcgata ggtggtgcgt cgtatgctcg
    17461 aggtctggcc gtgcgtctct ccactcgatc gtccctggtt ccccccgccc tagtccgctg
    17521 gcgcgtagtt gtgccgcttg ctttcggcat cacctgcgcc tgtgctcccg tccaggggcg
    17581 aaagccgacg agcgctccac cagtcatcgc cgtgccgaaa accatcgtga cgcgcaccag
    17641 cgcgacgagc atcccggagc tgtatgaaga agcctggtca gcctcgcgtg cgggagagca
    17701 cgcgcgcgcg gccgagctgt tcgagaaggt ctactcgctc gagcccgagg ggcctctcgc
    17761 ggacgacgca ttgtttcaat ccgcggcgga gtccgacgca gctgcgctgc tcgaagcggc
    17821 cgcgtcgaaa taccgagagc tgtgggaacg ctatcgcgag agcccgttgt caggaaccgc
    17881 cgcggtacgg gcgctgcgga tcctcgttca tctggaggca ctggatcggg cagagccgct
    17941 cgctcggcag ttgctcgatg gcaacgttac tctgctgcct ggcgagcgcg tcgtagtgcg
    18001 cggtgcacta gcgctctccg cgctcgcccg cgacgcggaa accgacgctc gtcgccacat
    18061 cgaggagggg cgcaacatcg tcgacgcgca acaattggat tcggcagatc gcgtctcgcc
    18121 tgacctcgcg ccgctctatt tcgcgctcgg tgagctgcgt cgacggctgg gcgatcgcat
    18181 cgtcttcgag ccgatgcccg tcaatttccc ggccgcactg gagcagcgtt gtcagcacct
    18241 gctcgacgca cagagcgcct atctcgacgc gattcgcgcg cgtgacgcgc actggtccgc
    18301 gatggcgggc ttccgagttg gtgagctgta tcagactctg catcgagacc tcatgaaggt
    18361 caccccgccc gcgagcgccg acacggaagc caagcgtcag ctgttcgaag gcgccatgcg
    18421 cgttcgatat tcgatcctgc tcaccaaggc gcgcgccatg ctcgagcaca cgttagccat
    18481 ggccgagcgc accgcagagc attcgcgctg ggtagacaga gcacgcgaag caagccaagc
    18541 cattcagcgc gcggtcgaag aggaaaacgc tgccctcgat cggctgccct attcgcgaga
    18601 aacactcgcg gccgcgctcg ctgacctgga gaagcggagc gccgagaagg cggctacgaa
    18661 caaggcgggc cccgtgaaac ccggctcggc caatccggga aagtcgacgc caagcatgcg
    18721 gccatagacc gaaagccaag gccgcatgcc tcgaaggacc aacgtgggtt cgaagcaaaa
    18781 cggcgcgggc tcctcgcatc ggagctcctc gcgataaggg aacttggaga aacgattggc
    18841 cagggtcagc cgagagtgct tcaacccccg caagcaatcc gagcacaaag cccgcgaatt
    18901 ctgagctgtt tgcgggcggg tatgaaatat gcttgacagc ccgaggccct ggcgctacca
    18961 gagcgcggtc tcgtcggacc ccgctcgtac ctcattgccc tcgctcatta gagtccagcg
    19021 cttaccagca cggcctctat aactagcacc ggctgaccgc ttaggcggtt ctcggaaagg
    19081 caagggaggg gcaggttggg gtgcctcttt cgagcgggcc cagcactcgt ttaaggaaag
    19141 tctgggcttg cctcaccgcc tcaagcgttt ttctcgtcgg aacttgctca tgcaagctag
    19201 cggctcccgc caagtgtcgc cgaaaggctt ccccggtctc gaccggggcg tggcgccgat
    19261 ccgccgaaaa tctgctggcg tagctcaatc ggtagagcac ctgttttgta aacaggtggt
    19321 taggggttca agtcccctcg tcagctccgc cagtcgttgt atctctgccg tcttttctga
    19381 tagtcgtccg tttccgtttt ctgagtctct gtccgttagt cctcgtcacg aggaattacc
    19441 tgacctttcg gagggttgcc cgagcggcca aagggagcag gctgtaaacc tgccggctca
    19501 cgcctacgtt ggttcgaatc caacaccctc cacaaacccg aacgatgcac tccccgtgcg
    19561 ttcagtgtcg ggctctgcag gtcgtctggt cacggcggtg gttcgtcgcc gccgtggttc
    19621 gtttgccccg tgttccacca cgtctagtcc aactgcggga gtagctcagt tggtagagcg
    19681 tcagccttcc aagctgaatg tcgtgggttc gaatcccatc tcccgctctg ttcttcctca
    19741 atgcccgtct tctcctcctc gggcgttttt tcacgatttt gcacgacggg gttgccagag
    19801 cccgcaggcg tgcgaaaatc cggcccccct ggggtcagcc caggttacag attcccccag
    19861 gcccacatag ctcagatggt agagcacgtc cttggtaagg acgaggtcac cagttcaatc
    19921 ctggttgtgg gctcgagcac tctctccctc ttcaagcgta ccctttagcg agaatagttc
    19981 cccggaaagg ttgtcatggc caaggagaaa ttcgtacgga ctaagcccca cgtgaacgtc
    20041 gtgacgattg gtcacatcga ccatggcaag acgactttga ctgccgcgct ggtgaaggtg
    20101 cagtcgaaga agaacatggc gaaggagatc aagtactccg acatcgcgaa gggcggcatc
    20161 gttcgtgacg agacgaagac ggtgacgatc
//
LOCUS       DASTKU010000526         6315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_548194, whole genome shotgun sequence.
ACCESSION   DASTKU010000526 DASTKU010000000
VERSION     DASTKU010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548194"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            418..1185
                     /locus_tag="VFQ61_18190"
     CDS             418..1185
                     /locus_tag="VFQ61_18190"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9956444.1"
                     /translation="MARAAATSHSLNDAIQELMAARDEARLQAHLFSLDARRSWDELE
                     NSLAELEQRLGQSSGRMAETALTKTVELTQSVRAFVSRHGRHRSELIIPARAVMSQPV
                     TTCAPEDTLHRAAQLLWDYDCGALPVVERSGRLAGMLTDRDICMAAFTRGQPLWSCTV
                     ESAMSRPVHCVTTDASVQQVLALMRDKRVRRVPVVNEAGFIVGLISFADLLQKLPALD
                     DETAARVAIAEAMGAVCAPPEGGPKSTSRIPLPPRVG"
     gene            1565..2728
                     /locus_tag="VFQ61_18195"
     CDS             1565..2728
                     /locus_tag="VFQ61_18195"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9956445.1"
                     /translation="MSGPFEFVVFDQSCSVQVFELCARIASELGPTLLHTGTDFSGES
                     KPGLTVIRGPAYDNRSYSSRLRTWSAYVRHALNVGLRLRGRPVVMVSSNPPFGPFVGY
                     TLRKLKGFRYLVRILDVYPDAIVKAGLATGIWRLLPATWERANRIALESAEHVVTLGE
                     CMRDRVQRFVTAPVRIIPSWLDVHEIAPRPKAENWFAQQHDQLGKLTVIYSGNLGITH
                     DFSGVFEAARRLRERSDIQFVFIGGGGRKDEISAATRGLPNCRVLPNQPKENLPYSMT
                     CGDVAIVALGESGQGISMPSKTYHMMSAGAAILGISFGDNDLKRTIEHHQCGINVDPR
                     DAQAVVAAVERFRADPEFLSRCRINARMAAEREFSAQVCIPQYLELLDDMRHK"
     gene            complement(2753..3262)
                     /locus_tag="VFQ61_18200"
     CDS             complement(2753..3262)
                     /locus_tag="VFQ61_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956446.1"
                     /translation="MAPAPRFVWADTGSFRIMVHGEEPPTDEEWARFMAATVREVEGQ
                     LRAPERKGAVIYSLGGMATPKQRRELQRLQFAGPPPTLVLMSDSRFARGVATALGWLM
                     PSLKNFHALALQEVEKAASLLSAHAGERSEFKHVLAELLRELQGSGPPASSAREWPRS
                     VRPGDASAR"
     gene            3632..6052
                     /locus_tag="VFQ61_18205"
     CDS             3632..6052
                     /locus_tag="VFQ61_18205"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HET9956447.1"
                     /translation="MKTAPNHSSKLPQSTLHQPTTRARARRTIRATCCVAVTACFACA
                     PKQAVVEAPPASGAAVSTVGAPKSGELLQRSSFEDGATLPWMPLVLPPASGEAVTKDG
                     AYCMRVERAGKNSWDLQIRHREMTIVKGHHYTARFKAWASAPVKLRAQIGMSGPPYAQ
                     YWGKSLELGTEPTVLSESFSSSAADDPTAEFAFHIGDASTQAPVTVCIDDIHLTDPEF
                     VAVAKPKMPEPPGIRLNQLGYAPQSAKRATWVVGSQEGAGAQKRVPFELVDAAGKVVH
                     RGVTEPFGKDPTSGLFVQRIDFSSYSAPGEKLRIRLTNGDSRIESDPFSITASPLKQL
                     SRDALRYFYYTRSGIALEQPFVEDARWVRVVGHPTDSQVPCAPDAKCTYSLDVSGGWY
                     DAGDYGKYVVNGGLSAWLLTNLYEAAQQLKLTQVVGTKDNDLHLPESGNGTPDVLDEV
                     RWELEWMLKMQVPAGDAQAGLVHHKIHDVDWSSLGTLPLLQSATPRQLRPVSTAATLN
                     LAAAAAQAARVFSTLDPKFAARCLAAAKRAWVAAEANPKLLITGADNHGGGAYEDDDV
                     SDERFWAATELFITTGEKAYFTASSESPHFSKVPAPRAGAPQSLDWRSTSALGTLSLA
                     LDERHTPAKVRDASRKAVIEAADKYLSLVKADAFGQPYAGAHYPWGSNSFIVNNGLIL
                     AYAHAFTHDARYLEGAQAALDYVLGRNALGKSYVTGYGARPLVNPHHRLWAHSIDAKF
                     PPPPPGVLSGGPNSDLQDPYSKGLHPHCVGQTCFVDHIEAYSANEVAINWNAAFAWLT
                     GYLNVTTK"
BASE COUNT         1130 a         2002 c         2026 g         1157 t
ORIGIN      
        1 tactagtagc ctctgctcca gcagcaattg ccgcaggtca ccgatgggag acgctccccg
       61 tggcagggaa tgcaggcaat gctgaccgga cgaatcgcga gtgacgggcg ctgattagcg
      121 cagctttctg catcgcgcaa atccagcggc ccttccgggc tagtttcgga gaactttgcc
      181 gaggtttggg ccaggcgcgc gcgaagggcc gcgctaattc ttcgcggcct ccgcgcaaag
      241 agcgggcaac tccctggtgg cccgaggcgt gcatgaccga gcgcaggggc gcactgtcgg
      301 caccccgagc gctgccactt tcgcacacct cgtgcgagct cgctcgttcg cccaccgtgc
      361 gttgaaaccc accaggcggc tctgccgctg ggcgagttca agaaggagga aatcagaatg
      421 gccagggccg cagcaacttc gcattcgttg aacgacgcaa tccaagagct gatggcagca
      481 cgcgacgaag cgcggctgca ggctcacttg ttctcgctcg acgcccgacg cagctgggac
      541 gaactcgaga acagcctcgc tgagctcgag cagcggctcg gccaaagcag tggcaggatg
      601 gccgagactg cgttgaccaa gacggtcgag ctcacgcaaa gcgtgcgggc cttcgtcagt
      661 cgacatggtc ggcaccgctc ggaactgatc attccagcga gggccgtgat gagtcagccg
      721 gtgactacct gcgctcccga ggacactctg catcgcgctg cgcagctact ctgggattac
      781 gattgcggtg cgctgcccgt cgtcgaaagg agcggtcggc tcgcggggat gctcacggat
      841 cgcgacattt gcatggccgc gtttacccgg ggccagccct tgtggtcgtg cacggtcgag
      901 agcgcgatgt ctcgccccgt gcattgcgta acgaccgacg cttcggtgca gcaagtgctg
      961 gctttgatgc gcgacaagcg cgtgcgacgt gtgccggtag tcaatgaggc gggtttcatc
     1021 gtgggcttga tctcgttcgc ggatctcctc caaaagctgc ctgcgctgga cgacgagacc
     1081 gcggcgcggg tcgccatcgc ggaggccatg ggcgcggtgt gcgctcctcc cgaaggcggg
     1141 ccgaagtcga cgtcgcgcat tccgctcccg ccacgagtcg gttgacgcga acctttttgg
     1201 gccggctcta cctggtccaa atctgagccg ttcgcttcgc gttggttcga ttcgacgtat
     1261 gccccacgga tggggatgtt ccgggcttgc gccgcgtctc cttcggcgca agcctgcccg
     1321 gcagccgggc tgcggctgca cagtggccgc atcgtattgg ccggcagagt gttggcaggg
     1381 gggaaagcga ggtggtggcg aactcgtcgc ctaaggcttg actgcagacc ctcggcagtt
     1441 tggcgatcga ggaaatgtgg cgtgcttcgt tggtgtttgg ggtttcctgc ggctaagctc
     1501 cggcccctcc gaatggctaa gggcctcgga tatcgttgag ccaacgggta tcgcagatcc
     1561 aaaaatgtcc gggccgtttg agttcgtcgt tttcgaccag tcctgcagcg tgcaagtttt
     1621 cgagctctgt gctcggattg cttcggagct tgggcccacg ctgctccaca cgggaacgga
     1681 tttctccggc gagagcaagc ccgggctcac ggtcattcgc ggcccggcct acgataaccg
     1741 cagttactcc tcacggctga gaacgtggtc cgcttacgtg cggcacgcct tgaacgtggg
     1801 gctgcggctg cgcgggcgtc cggtcgtgat ggtgagttcg aaccctccgt tcggcccctt
     1861 cgtaggctac accctgcgta agctgaaggg gttccggtat ctggttcgca tcttggacgt
     1921 ttaccccgat gccatcgtca aggcgggact agccacggga atctggcggc tgctgcccgc
     1981 cacctgggag cgcgcgaatc gaatcgcgct cgagagcgct gagcacgtgg tcacgttggg
     2041 cgagtgtatg cgcgatcggg tgcagcgttt cgttacggcg cccgtgcgca ttatcccgag
     2101 ctggctagac gtgcacgaaa tcgctccgcg acccaaagcg gaaaactggt ttgcccaaca
     2161 gcacgaccaa ctcggcaagt tgaccgtcat ctactcggga aatctgggca taacgcacga
     2221 tttctcgggg gtgttcgagg cggcccgacg cttacgcgag cgctccgata ttcagttcgt
     2281 gttcatcggg ggcggcggcc gcaaagacga aatcagcgcg gccactcggg ggctgcccaa
     2341 ttgccgcgtt ctgccgaatc aacccaaaga gaatctgccc tactcgatga cgtgcgggga
     2401 tgttgcgatt gtggccttgg gagagtctgg gcaaggcatt tcgatgccga gcaagaccta
     2461 tcatatgatg tcggccggcg cggccatcct gggcatttcg ttcggtgaca acgatctcaa
     2521 gcgcaccatc gaacaccatc agtgcggcat caacgtcgat ccgcgcgacg cgcaggcggt
     2581 ggtcgccgcg gtcgagcgct ttcgcgccga ccccgagttt ctcagccgtt gtcggatcaa
     2641 cgcccgaatg gctgcggaac gcgagttttc ggcgcaagtc tgtattcccc agtacctcga
     2701 gctgctcgac gacatgcggc acaagtaaac tcgagcgccg cctcccgcat cttcaacgag
     2761 cggaagcgtc gcccgggcga acggagcgcg gccactctcg cgccgaggag gcgggcgggc
     2821 ccgagccctg cagttcgcgc aagagctcgg ccaatacatg cttgaactca gagcgctcac
     2881 cggcgtgcgc gctcaacaag ctcgccgcct tctccacctc ctgcaacgcg agcgcgtgga
     2941 agttcttgag ggagggcatg agccagccga gcgccgtcgc gacgccgcgg gcgaagcgtg
     3001 aatcactcat caacaccagc gtcggcggcg gccctgcgaa ctgcagccgc tgcagctcgc
     3061 gtcgttgctt gggcgtagcc atgccgccga gcgaataaat cacggcgccc ttgcgctccg
     3121 gcgctcgcag ctgaccttcc acctcgcgca ccgtcgccgc catgaaccgc gcccactctt
     3181 catccgtcgg tggttcttcc ccgtgaacca tgatgcgaaa actgccggta tcagcccaga
     3241 cgaaacgtgg tgccggagcc atcgtcgggt caacatagca cagcgtgcgc ggtcctcgaa
     3301 aatgaggggt tcgagccggg ccgcggagga atcgtgcgtc gaactcgcga gtcgggaccc
     3361 gaggtctcgg cgactcgaag tcgatacgag cagagttcgg ggcgggtcga atcggagccg
     3421 tcgggcgtag ccccgcgggc ttgagctttt ctgctcgtgc tttgagctcg aacgcactcg
     3481 agctcaccgc ggagggggtg attcggggcc ggcggtcgag gatttgcgct ttgcgcgcga
     3541 atccgtgatc cgagacctgg ggtgtacaca ttccatggca gacgctgctg ggcgtcgagc
     3601 tccgagctct caacgagcgc caaaaattgc catgaaaact gcacctaatc actcttccaa
     3661 gctcccgcag tcgaccctgc atcagccgac cacccgcgcg cgggcgcgcc gtacgattcg
     3721 cgcgacctgt tgcgtcgcgg tgaccgcctg ctttgcctgt gctccgaagc aagccgtggt
     3781 ggaagccccg cccgcttcgg gagcggccgt atccacggtg ggggcgccga agagtggtga
     3841 gctgctgcag cgttctagct tcgaggatgg cgcgactctg ccctggatgc ctctggtgct
     3901 tccacccgcg agcggcgagg cggtcaccaa ggatggcgcc tattgcatgc gtgtcgagcg
     3961 cgccggcaag aacagctggg acctacagat tcgtcaccga gagatgacga tagtcaaagg
     4021 ccatcactac acggctcgct tcaaagcgtg ggcctcggcg cccgtgaagc tacgcgcgca
     4081 aatcggaatg tctgggccgc catacgcgca atattggggt aagagcctcg agctcggcac
     4141 cgagccgacc gtgctcagtg agtccttctc ttcgagtgct gcggacgacc ctaccgccga
     4201 gttcgctttt cacattggcg atgcgtccac gcaggctccc gtcaccgtct gcatcgacga
     4261 cattcacctg accgatcccg agttcgtcgc ggtcgccaaa cccaagatgc cggagccgcc
     4321 cggcatccgc ctcaatcagc tgggctatgc tccacagagc gccaagcgag ccacctgggt
     4381 ggtcggctcg caagaaggtg cgggagccca gaagcgcgtg ccgttcgagc tcgtggatgc
     4441 tgcgggcaag gtcgtgcatc gcggcgtcac cgagccgttt ggcaaagatc ccacgagcgg
     4501 cctgttcgta cagcgcatcg atttctcttc ctacagcgcg ccgggtgaaa aactccggat
     4561 tcgcctgacc aatggcgaca gccggatcga gagcgatcct ttctcgatca cggctagccc
     4621 cttgaagcag ctctcgcgag atgcattgcg ctatttttac tacacgcgga gcggcatcgc
     4681 gctcgagcag ccattcgtgg aagacgcgcg ctgggttcgc gtcgtggggc acccgaccga
     4741 cagccaggta ccctgcgcgc ccgacgcaaa gtgcacctat tcgctcgacg tgagcggcgg
     4801 ctggtacgac gcgggggact acggcaaata cgtggtcaac ggcgggctca gtgcctggct
     4861 tctcaccaac ttgtacgagg ccgcgcagca gctgaaattg acccaagtcg tgggtaccaa
     4921 agacaacgac ctgcacctac ccgagagcgg aaacggcacg cccgacgtgc tcgacgaggt
     4981 gcgctgggag ctcgaatgga tgctgaaaat gcaggttccc gcgggtgacg ctcaggcggg
     5041 gctcgtgcat cacaagattc acgatgtgga ctggagttcg ctcggtactt tgccgctgtt
     5101 gcagtcggca acgccccggc aattgagacc ggtgagcacc gccgcgacct tgaacctggc
     5161 ggcggcggcc gctcaggcag cgcgggtgtt ctccactctg gatccgaagt ttgccgcgcg
     5221 ttgcttggcg gcggccaagc gggcttgggt cgccgccgaa gccaatccga agctgctgat
     5281 caccggagct gacaatcacg gcggcggcgc ttacgaggac gatgacgtca gtgacgagcg
     5341 cttctgggcg gcgaccgagc tcttcatcac cactggcgag aaagcttact tcacggcatc
     5401 gagcgaaagc ccgcatttct cgaaagtgcc cgctcctcgg gcgggagcgc ctcaatcatt
     5461 ggattggcgc agcaccagtg cactcgggac cctatcgctc gcgctcgacg agcggcacac
     5521 accggcgaag gtaagagacg ccagtcgcaa agccgtgatc gaggcggccg acaagtactt
     5581 gtccctggtg aaggcggatg cttttgggca accgtatgcg ggcgcgcact acccgtgggg
     5641 atcgaactcg ttcatcgtga acaacggcct cattctcgcc tacgcacacg ccttcacgca
     5701 cgacgctcgc tacctcgaag gggcgcaagc ggcgctggat tacgtgctcg ggcgtaacgc
     5761 gctcggcaaa tcgtatgtca ctggctacgg tgcccgcccg ctcgtcaatc cgcatcaccg
     5821 cctctgggcc cactcgatcg atgccaagtt tccgccgccc cctcccggag tattatcggg
     5881 tggccccaac tcggatttgc aggaccccta ttcgaaggga ctccatccgc actgcgttgg
     5941 tcagacttgc ttcgtggatc atatcgaggc ctatagcgcc aacgaggtcg ccatcaattg
     6001 gaacgccgcg ttcgcctggc tcaccggcta cctgaacgtg acgaccaagt agacagcacg
     6061 attctcctcg aaacgagcgg cggcgtcagc gcacgttggc ccgccgctcg cgctgcttgc
     6121 gctcttggtc aggctcgcgc tatcgcgaga cgagctcgcg ttcgatagcc gaggtgacga
     6181 gctctgaatt gggcggcacg tcgggcgcga agcgctccac gacctcgccc tggcgattga
     6241 ccaggaactt ctcgaaattc cagagcacct cgtggggcgc tcctggatcg atgccgtggg
     6301 cccggagctt ggctc
//
LOCUS       DASTKU010000527         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1663835, whole genome shotgun sequence.
ACCESSION   DASTKU010000527 DASTKU010000000
VERSION     DASTKU010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663835"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..561)
                     /locus_tag="VFQ61_18210"
     CDS             complement(109..561)
                     /locus_tag="VFQ61_18210"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HET9956448.1"
                     /translation="MTPKSSARTSTGLREKGCNSGCFRNAWTQVMQGGYTYRRRGVRH
                     VDSGPAPCDSVRMLFMVIERFRDNDMVPVYRRVRDEGRSLPAGLRYVDSWVEPNFSRC
                     FQLMECDDLRQLQQWVLERRGTGISFEFIPVVSSKETQETVRPYLDKP"
     gene            587..1354
                     /locus_tag="VFQ61_18215"
     CDS             587..1354
                     /locus_tag="VFQ61_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956449.1"
                     /translation="MPCSTGFFQRSSILLLALAPSCLPYVVPPARVSVGGATQSGRVL
                     VDGETRARPNATVIRAGLHPLDVADKELPVDVGLGYQAELTSVDGVPTVQGMYLEVGA
                     YPLILPLGERVSLRAGGYGAVEGLARSSLEDTGLGGTVGGMLELTGRVSSPYAGADSD
                     GTLHAGFARGRWAVGLFGSGSVRNFGDGGYESATVGVSVRLPLLAGVVCCALPSLSHG
                     GHGSSHSSSSSSSSGSDSSQSQSHRLVRTPASPRPKE"
     gene            complement(1406..2251)
                     /locus_tag="VFQ61_18220"
     CDS             complement(1406..2251)
                     /locus_tag="VFQ61_18220"
                     /inference="COORDINATES: protein motif:HMM:NF023385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HET9956450.1"
                     /translation="MFTINAITVDVEDWAQSTLNPEYPLSSRFVESTREMLELFERHR
                     VKATFFVLGLAAQKAPGLVREIHRRGHEIQSHGFGHRSLFSLTRSELKADLERSKKLL
                     EDTVGEAVTGYRAPSFSVTTETLWALDVIFECGFTFDSSIFPVKTARYGIDGAPRVPH
                     WLETPAGNRLLELPVASARLLGRTLPGGGGGYLRLLPYALTQCGVRQLNRSGHPATLY
                     LHPYELDVREMLEVRGVPWMLRAQQGLGRSGVAGKLNRLLAEFEFTTVTRAMASCGDL
                     PRFRH"
     gene            complement(2285..>2719)
                     /locus_tag="VFQ61_18225"
     CDS             complement(2285..>2719)
                     /locus_tag="VFQ61_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956451.1"
                     /translation="GQVVGHVAVATHVRALALEFLRLHWGAALRCAGRRLLSAGTLSR
                     AFATLSYTLRPKLPRLPQAEILTLSVSERHARLGLLGLIYESLVAQVQSRGVTALRIV
                     TAVRLGRLHGFLERRGWQHRYTMTLHPGEASRVYVLESLGAS"
BASE COUNT          536 a          855 c          851 g          477 t
ORIGIN      
        1 gcgcgatcgc ggtgaatgct gccgctccgc ttgccggttc tcgttcgcgc actctcagga
       61 tggccaggcg gagaacctcg acaaccaccg caaacggatc cgtgttgctc agggcttgtc
      121 cagatacggc cgcaccgtct cctgcgtctc tttactgctc acgaccggaa tgaattcgaa
      181 agagatcccg gtgcctcggc gctccagcac ccattgttgg agctgcctca gatcatcgca
      241 ctccattagc tggaagcacc gcgaaaagtt cggctcgacc cagctatcca cgtacctgag
      301 accagcgggc aggctgcgac cctcgtcccg aactcgtcgg tagaccggaa ccatatcgtt
      361 gtcacgaaag cgttcgatga ccatgaacag catgcgcaca gagtcgcacg gcgccggccc
      421 ggagtcgacg tggcgcacac cacggcgccg ataggtgtat ccgccctgca tcacctgtgt
      481 ccaagcgttc ctgaaacacc ctgagttgca gcccttttcc cgaaggccag tgctggtacg
      541 ggcgctgctc tttggggtca cgtcgctcgc taccgcggtt tcccctatgc cttgttcaac
      601 tgggttcttt cagcgttcct cgatcctact cctcgcgctc gcgccgtctt gcttgccgta
      661 cgtcgtgccg ccggcgcgcg tatcggtggg cggtgcgacg caaagtggac gagtgctggt
      721 agacggcgag acgcgcgcgc gaccgaacgc gaccgtgatc cgcgcggggc ttcatccgct
      781 cgacgtcgcg gacaaggagc tgcccgtaga cgtcggcctg ggttatcaag cggagctcac
      841 atcggtcgac ggcgtaccga ccgtgcaggg catgtatctc gaggtcggag cttacccttt
      901 gattttgccg ctcggagagc gggtgagctt gcgcgcgggt ggatacggcg cggtggaggg
      961 actcgcgcgc tcgagtctcg aggacaccgg tctaggcggc acggtgggtg gcatgctcga
     1021 gctcacgggg cgagtgagct caccgtatgc gggagcggac agcgatggca cgctccacgc
     1081 cggcttcgcg cgcggacgct gggcggtggg cttgttcggc agcggttcag tgcggaactt
     1141 tggagacggc ggctacgaat cggccacggt cggcgtcagc gttcgcttac cattgctcgc
     1201 cggcgtcgtc tgctgcgcgc tgccgagtct ctcccatggc ggccacggct cgagccacag
     1261 ctcatcgagc agcagctcat cgggcagcga ttcctcgcaa agccagtcgc accgactcgt
     1321 tcgcacaccg gcgagtccgc gaccgaaaga gtgagcgaca ggcaccgagg tgcagctgcg
     1381 caaaaccgaa aactgcgcgt accgatcagt ggcgaaaacg aggaagatcg ccgcagctag
     1441 ccatggcgcg agtaacagtg gtgaactcga actcggcgag caaacgattg agcttgcccg
     1501 caacgcccga gcgtcccaag ccttgctggg cgcgaagcat ccaaggcacg ccccgcactt
     1561 ctagcatctc gcgaacgtcg agctcgtaag gatgaagata cagggttgca gggtgcccgg
     1621 agcgattgag ctgacgcacg ccacactggg tcaacgcgta tgggagaagt cgcaagtaac
     1681 cgccgcctcc tccaggaagc gtgcgcccga gcagccgggc gctcgccacg ggcagctcga
     1741 ggagtcggtt gcccgccggc gtttcgagcc aatgcggaac gcgcggagcc ccgtcgatac
     1801 cgtagcgcgc tgtctttaca gggaagatcg aagaatcgaa tgtgaatcca cattcgaata
     1861 tgacgtcgag cgcccaaagc gtttcggtcg ttacggaaaa gctgggagcg cggtagccgg
     1921 taaccgcttc gcccacagta tcttcgagca acttcttcga acgttccagg tccgccttca
     1981 attcggagcg tgtcagagaa aacagcgaac gatggccgaa gccgtgactt tgaatctcgt
     2041 gcccgcggcg atggatctct cgcaccaagc ctggcgcctt ttgagctgcc agcccaagaa
     2101 cgaagaaagt cgccttgacg cgatgtcgct cgaatagctc gagcatctca cgggtgcttt
     2161 cgacgaagcg agagctcagc ggatactcag gattcagggt tgactgcgcc cagtcttcga
     2221 cgtcgacggt gatggcgttg atagtgaaca tggcgcccga atcgatgatg gctagcctcg
     2281 cacgctagct cgctcccaag gactccaaaa cgtagacgcg actcgcctcg cccgggtgca
     2341 acgtcatggt gtagcggtgc tgccaacccc gtcgctcgag gaaaccgtgt agacggccta
     2401 ggcggacagc agtcacgatt cgcaatgcgg tcacgccacg ggattggacc tgcgcaacta
     2461 gagactcgta gatcaaacca agcagcccca atcgagcgtg gcgctccgac acactgaggg
     2521 tgaggatctc cgcttgcggg agccgcggaa gctttggacg cagtgtatag gacagggtcg
     2581 caaaagctct gctcaacgtc ccagcggaca gcaaacgacg gcccgcgcag cgcagcgcag
     2641 ctccccaatg caatcgtaag aactcgagcg caagagctct gacgtgcgtc gccaccgcca
     2701 cgtgtccaac cacctggcc
//
LOCUS       DASTKU010000528         6553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_102915, whole genome shotgun sequence.
ACCESSION   DASTKU010000528 DASTKU010000000
VERSION     DASTKU010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102915"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFQ61_18230"
     CDS             complement(<1..380)
                     /locus_tag="VFQ61_18230"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET9956452.1"
                     /translation="MAEITDKAREDFFSEAQEIIETLSRNLLSLDAASRQGSIDPGLI
                     NEAFRAVHTLKGLSGLFGAVRLGALSHRLEDLLDSLRLGRMGLGPDVLDLLFSAVDIY
                     GKVLEFEKAGKDESLPELDELVGS"
     gene            complement(373..849)
                     /locus_tag="VFQ61_18235"
     CDS             complement(373..849)
                     /locus_tag="VFQ61_18235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9956453.1"
                     /translation="MFVSRCIDRPPSFAMDDLMSSAVRARILVVEDSPSMRGFVRAAL
                     EGAQASLGPVEVSEAQSGFEALRLLPRGPYSLVITDVNMPDINGLELVQFIRNNEQHR
                     ATRIVIISTQSAERDRERGLKLGANAFLAKPFTVDAIVEMTRLQLAAATEPARPNG"
     gene            1077..2477
                     /locus_tag="VFQ61_18240"
     CDS             1077..2477
                     /locus_tag="VFQ61_18240"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET9956454.1"
                     /translation="MPTLSPNPRVCVIGAGISGLTSVKALLDLNLTSDRNLSCTCYEA
                     SDRVGGLWAFGNAGGNSAAYRSLFINTSRDRMQLRDFPMPREYPDYPHHELIGRYLRA
                     YAEQFQLQRSIRLNTRVTRVGPNPSGGFSVTTCPSRKTSDSEARKGGAPPPATSPDTT
                     TEHFDAVIVANGHHFSPRFPRPAPKGAFAGTTLHSHSYVDPQNPCDLSGKRVIVVGFG
                     NSAVDIACELAREPARAQVYLSVRRGAWVLPRYALGRPLDQVTLGSGLLPRAITQPLA
                     ELWYRVAIGDVTRFGLPQPDHGLGDAHPTMSDELLPMIGKGRIVAKPQIDRYEGNDVV
                     FADGSRESIDAIVFATGYDVSFPFFESSFVSAPDNELPLYLHVFAPEISGLYFVGLCQ
                     PLGPIFPLAEAQAKLIARHVAGHYSLPSVEQMRRATQRERALMRRRFGSSARHTMQVD
                     FDTYLSSLNRELERSA"
     gene            complement(2485..4101)
                     /locus_tag="VFQ61_18245"
     CDS             complement(2485..4101)
                     /locus_tag="VFQ61_18245"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9956455.1"
                     /translation="MQKAQLLREIRARFASLAQRMVDVANREKRVPAAETAAGEEWFS
                     GPVISLRALRLFEAALLDVSTHGVPRISEDRVVRHAGRTRVLLTPNDAYESVLYRGWR
                     SWVELDSEASKRDLRDSLATFYRQPAAPGRLVLVLGAGNVASIPVLDVLYHSFVEGSV
                     CLLKMSPVNAYLGPLLEAAFEPMVTRGYLEFVYGDGELGSHLTRELRVDAIHITGSLQ
                     THDTVVWGPPGPVRARQRELGEPLTKKRITSELGNVGPVFVAPAEYSDAELTRAAENI
                     AGMLVHNASYNCNAARVLVTARGFVQREELLRRIAAVLERTPTRVAYYPGARSRHEEL
                     LTRLAGAEVQRVGTADKERLPWTLVKSLSPDEECPLFEAEAFCPILGEVVLEADDAAD
                     FLPLAPEFANERLFGTLNAMWLVPSSLERDPTLFHALDEGIRALRYGTVSVNIWPAVG
                     YGMGLTPWGGFPGATLEDPQSGIGTGHNAALLDHTEKVILEGPLLGFPKPFWCPGHAH
                     LAELGAALAAFEAEPSFARATRAAWAGVRS"
     gene            4392..5522
                     /locus_tag="VFQ61_18250"
     CDS             4392..5522
                     /locus_tag="VFQ61_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956456.1"
                     /translation="MATFSGSERASEAPQAHSTRTSAAPASATSTTPPDALGTRQARP
                     SNPQQTHITATFEGIRHTQSGASLLLSRGDAHAHAALSACVRSEASLSTLLRSIQQLA
                     AHVSTAQAAKDDVAQEVEHLRSLLVYADEQQLALKHHNVALEQALRQTVAQAEAEAAR
                     ERAFLVEQQDAFIIKLLEDHELALTELRAKLRAAEADLIRYSEERRSHASTVRSATST
                     SFELTQKLGAAEQALARMQAERDRLLADAERRELENRELRQELETLRTAPVPSTAPTD
                     PPPIPPSRIPGSGGVPLAAVRLESRQAPPRPELRPEALGIRTEPYLSAATSPASAGPN
                     SAKPALKSKPDASTRPLIGYSLIGDELHEELVDSGRGRSPKS"
     gene            5658..>6553
                     /locus_tag="VFQ61_18255"
     CDS             5658..>6553
                     /locus_tag="VFQ61_18255"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9956457.1"
                     /translation="MQWSTKLFRIRGIDVRVHATFALIVALGATQWGARHGMGGAAFG
                     AAFTLALFACVLLHELGHSLVAQKFGLQVKEIILLPIGGIANLLGKPKHPGQEIAIAL
                     AGPAVNILLAGLFGAALWATGQHLGGARALVASAKLPSLSTFLLLLTTGNLSLALFNL
                     LPVFPLDGGRVLRALLELRWGAVRATVWAAGFGQVAALALGGYAVWSGQLFLAFIAAM
                     IFMSAARETASTQLHGPLTQLRAEQLAEMPAVEFDAHDRIRDAVPSLLRSAQTAFPVV
                     SGSEVAGVVLRQDLIRAAEQPET"
BASE COUNT         1227 a         2185 c         1982 g         1159 t
ORIGIN      
        1 aagctaccca cgagctcatc gagctcgggc aagctctcgt ctttgcctgc tttctcgaac
       61 tcgagcactt tgccgtaaat atccacggcg ctgaacagca aatccaagac gtcagggccg
      121 aggcccatac gcccgagccg cagtgaatcc agcagatctt ccaagcggtg cgacagcgcc
      181 ccgagccgaa ctgcgccgaa cagccccgac aaccctttca gggtatgtac ggcgcggaag
      241 gcttcgttga tcagcccagg atcgatcgac ccttggcgtg aagcggcgtc gagcgagagc
      301 aggttccgag acagggtctc gatgatttcc tgcgcttccg agaagaagtc ctcgcgcgcc
      361 ttgtcggtga tttcagccat tcggcctcgc gggctccgtg gcggccgcga gctgcagacg
      421 cgtcatctcc acgatcgcgt ctacggtgaa cggcttggcc aaaaacgcat tggcacccag
      481 cttcaaccct cgctcccgat cgcgttccgc cgactgagtc gagatgatca cgatgcgagt
      541 ggcacgatgc tgctcattgt tgcggatgaa ctgcaccagc tcgaggccgt tgatgtccgg
      601 catgttgacg tcggtgatca cgagcgaata cgggccgcgg gggagcaacc gcaaagcttc
      661 gaatccgctc tgtgcttcgc tgacctcgac gggacccaag ctggcttggg cgccctcgag
      721 cgcagcgcgg acgaatccgc gcatgctggg cgagtcttcg acgacgagaa tgcgagccct
      781 taccgccgag gacatcaggt catccatcgc gaagctaggg ggacggtcga tgcaacgaga
      841 gacaaacact ccatacgcag ggtcctttgg ccgctcgttc gaggaacgac ccgcctcgag
      901 ccccgaaggg tacgggattc ttggcagaat cagaagcccc tacggcttgt atttcgcctt
      961 tgcggccccg aacggatggc acccggtacg cggctcgccg ttgcgagcac accgggcgcg
     1021 ccgtatccct gcgcgcgcca cctctcggag caacacggcc cgtagacaaa agcccaatgc
     1081 ccaccctatc cccaaacccg cgtgtgtgtg tgatcggagc tggtatttcc gggctcacct
     1141 cggtcaaggc gttgctggac ctgaacctga cgtcggaccg gaacctgagc tgcacctgct
     1201 acgaagcgag cgaccgggtt ggcggcttgt gggcgttcgg aaacgcaggc ggcaactcgg
     1261 ccgcctatcg gtccctgttc atcaatacct cgcgcgatcg catgcaactt cgggatttcc
     1321 cgatgccgcg cgagtatccg gactatccgc accacgagct catcggtagg tacctccgcg
     1381 cgtacgccga gcaattccaa ttgcagcggt ccatccgctt gaacactcgc gtcactcgtg
     1441 tgggtcccaa cccttcgggt ggcttctcgg tcaccacctg cccgtcacgc aaaacttcgg
     1501 attccgaagc gcgcaagggc ggagcgccac cccctgcgac gagtccggac accacgacag
     1561 agcacttcga tgccgtgatc gtcgcgaatg ggcaccattt ttcgccccga ttcccgaggc
     1621 cggcgcccaa gggcgcgttc gccggcacta ccctccattc gcacagttac gtagaccctc
     1681 aaaacccttg tgatttgagc ggtaagcgag tcatcgtggt gggtttcggg aacagcgcgg
     1741 tcgacattgc ttgcgagctc gcccgcgagc ccgctcgggc ccaggtgtat ctctcggtgc
     1801 ggcgcggagc ttgggtactg ccacgttatg cgctcgggcg ccccttggac caggtgacgt
     1861 taggaagtgg tctgttgcca cgggccatca cacaaccact cgcggaactc tggtatcgag
     1921 tcgcaatcgg cgacgtcacg cgcttcgggc taccccaacc cgatcacggc ctaggggacg
     1981 cgcatcccac gatgtccgac gaactcttgc cgatgatcgg caaaggacgc atcgtcgcga
     2041 aacctcagat cgaccgctac gagggaaacg atgtcgtgtt cgcggatggc agtcgagaaa
     2101 gcatcgatgc catcgtgttt gcgaccgggt acgatgtcag ttttcccttc ttcgagtcct
     2161 cgttcgtgag cgcgcccgac aacgagctgc cgctctactt acacgtcttc gcgcccgaga
     2221 taagcgggct ctacttcgtc ggtctatgcc aaccgctcgg gcccattttt ccgctggcag
     2281 aagcgcaagc caagttgatc gcacgccacg tcgccggcca ctactcgctg ccgtcggtcg
     2341 agcagatgcg gcgagccacg cagcgcgaac gcgcgctgat gcgccgccga ttcggcagct
     2401 ccgcacgcca taccatgcag gttgacttcg acacgtatct ttcgtccttg aaccgagagc
     2461 tcgagcgctc cgcgtgagag ctcgtcagct ccggacgccg gcccacgccg cgcgtgtggc
     2521 ccgcgcgaag ctgggttcgg cctcgaatgc ggccagggcg gcaccgagct cggccaggtg
     2581 agcatggccg ggacaccaga aaggcttggg gaaaccgagc aagggacctt cgagaatcac
     2641 cttttccgtg tgatcgagca gggccgcatt gtgcccggtg ccaattccag attggggatc
     2701 ctcgagcgtc gcgccgggaa atccgcccca aggggttagg cccatgccat agccgaccgc
     2761 cggccagatg ttcacgctca cggtgccgta gcgcagcgct cgaatcccct cgtcgagcgc
     2821 gtgaaacaac gtgggatctc gctctaggct gctcggcacg agccacatcg cgttcagagt
     2881 tccaaacagc ctctcgttcg caaactccgg ggctagcggc aagaagtctg cggcatcgtc
     2941 cgcctccaac acgacctccc ctagtatcgg gcagaatgcc tcggcctcga acagcgggca
     3001 ctcctcgtca ggggagaggc tcttcaccag tgtccacggc aaccgttcct tatcggcggt
     3061 accgacacgt tgcacctcgg caccggcaag tctggtcagc agctcctcgt gacgcgagcg
     3121 agcaccggga taatacgcga cgcgagtcgg cgttcgctcg agcacggccg cgatacgccg
     3181 aagcagctcc tcgcgttgca cgaagccgcg cgccgtgacc agcacccgcg cggcgttgca
     3241 attgtacgag gcattgtgca cgagcattcc ggcgatgttc tccgcggccc gggtaagctc
     3301 cgcatcgctg tattcggcgg gcgccacgaa cacgggcccc acgttaccca gttcactggt
     3361 aatgcgcttc ttcgtcagtg gctccccgag ctcacgctga cgcgcccgaa ccggtcccgg
     3421 cggaccccaa accacggtgt catgagtttg cagtgagccc gtgatgtgaa tggcgtcgac
     3481 gcgcagctca cgtgtcagat ggctaccgag ctcgccgtct ccatagacga actctagata
     3541 gccccgcgta accatcggct cgaaggcggc ctccaagagc ggcccgagat aagcgttcac
     3601 cgggctcatc ttcaacaagc aaacgctgcc ctccacgaac gaatgataaa gcacgtcgag
     3661 aacgggaatc gacgcgacat tgccggcccc gagcaccagc accaggcgcc ctggcgcggc
     3721 aggttgtcga tagaaggtgg ccagcgagtc cctcaagtca cgcttcgaag cctcggagtc
     3781 gagctccacc caagatcgcc agccgcgata cagcaccgat tcataggcgt cgttcggcgt
     3841 caggagaacc cgcgtgcgac cagcgtgtcg taccacgcgg tcttccgata tccgtggtac
     3901 gccgtgagta ctgacgtcga gcaacgcggc ttcgaacagg cgcagggccc ggagcgaaat
     3961 cacgggcccc gaaaaccact cctcgccggc cgccgtttct gccgcgggca cccgcttctc
     4021 tcgattggca acatccacca tgcgttgcgc caggctcgca aagcgtgcgc ggatttcccg
     4081 cagcagctgc gccttttgca aagggggtca gcgcgcaaac tcggccttcc gatccgtgag
     4141 ccgtagcagc tttgcttgca gttctgtttc agaagtattg ctgcgatcca tgcacgagca
     4201 gtcagcttaa agggaatctc aacccggcgc atcaactggg actttagcca ggcatctcga
     4261 gcgcccgagc ccccttccct tccccaaccg cctcccctag gatgccgaag gctgcccgcc
     4321 ttggcggagt gaccgaggtg ggcgcagcga gagcaactga tggtcaagag taagacggcg
     4381 gagagcaact gatggcgacg ttctctggtt ccgaacgcgc gagcgaagca ccgcaagccc
     4441 attcgactcg aacgagtgct gcccccgctt cggccacgtc caccactccg cccgacgcgc
     4501 tcgggacgag gcaagcgcgc ccctccaacc cgcagcaaac gcacatcacg gctactttcg
     4561 agggaattcg ccacacccag agcggcgcca gcctactgct cagccgtggc gatgcgcacg
     4621 cgcacgctgc actttcggcc tgcgtgcgca gcgaggccag cctgagcacg ctgctgcgca
     4681 gcattcagca attggccgcc cacgtgtcga ccgcgcaagc cgccaaagac gacgtggctc
     4741 aggaagtcga acacttgcga tcgctgttgg tctacgccga tgagcagcag ctcgcgctca
     4801 aacatcacaa cgtggccctc gaacaagcat tgcgccaaac ggtcgcgcaa gccgaagcgg
     4861 aagccgcgcg cgaacgagcc tttctggtcg aacagcaaga tgccttcatc atcaagttgc
     4921 tcgaagacca cgagcttgct ctaacggagc tccgcgcgaa gctgcgcgca gccgaagccg
     4981 acttgattcg ctactcggag gagcgtcgta gccatgcgag caccgttcgt tccgcaacct
     5041 cgaccagctt cgaactgacg cagaaactcg gcgcggcaga gcaggccctg gcccgcatgc
     5101 aggcagaacg ggatcgcttg ttggccgacg cggaacgccg agaactcgag aatcgagagc
     5161 tgcgccaaga gctggaaaca ctgcgcacgg cgccggtgcc atccacggcg cccaccgacc
     5221 ctccgccgat tccgccttct cgtattccag gttcgggagg tgtgccgctc gccgccgtaa
     5281 ggctcgaaag ccgtcaagcg ccccctcgtc cggaattgcg gcccgaggcg ctgggcattc
     5341 gcacggagcc ttatctgagc gccgccacga gccccgcatc cgcagggccc aattccgcaa
     5401 aacctgccct caagtccaag cccgacgcgt ccacgcggcc cttgatcgga tattcattga
     5461 tcggagacga gctacacgaa gagctcgtgg atagcgggcg cgggcgctca ccgaagagct
     5521 gacacgcacg ccacgcgacc tgtaggcact ggttacagat cgtcgccaaa gttcgtactt
     5581 aacgacttgg cttcgcgcct tagattgtcc agagccagct cgggttcggg cgggcttttc
     5641 tcaaaggaac gacgcacatg cagtggtcga cgaagctgtt tcgtattcgt ggcatcgatg
     5701 tccgggtgca cgccacgttc gcgctgatcg tggcgctcgg cgccacccag tggggcgccc
     5761 gacacggaat gggcggcgcc gcgttcggcg cggcattcac gttggcgctg ttcgcgtgtg
     5821 ttctgctaca cgagctcgga catagcttgg tggcccagaa gttcgggctt caagtcaaag
     5881 aaatcatcct cctcccgatc ggaggcatcg cgaacctgct cggcaaaccc aagcatcccg
     5941 gtcaggagat tgcgattgcc ctggccgggc cggcggtgaa catccttctg gcagggctct
     6001 tcggggcggc gctgtgggcc accggtcaac acctcggcgg cgctcgagcg ctggtcgcca
     6061 gcgccaagct gccgtcgctc tccacgttcc tgttgctgct caccacgggc aatctctcgc
     6121 tggctctgtt caatttgctg cctgtgttcc cgctcgatgg tggccgcgtc ctgagggcgt
     6181 tactcgagct gcgttggggc gcggtccgag ccacggtgtg ggccgccggc ttcggacaag
     6241 tcgcggcgct cgcgctaggt ggttatgcgg tttggagcgg tcagctgttc ttggccttca
     6301 tcgccgcgat gatcttcatg tcggcggcgc gcgagacggc cagtacccaa ctgcacgggc
     6361 ccctcaccca gcttcgcgcc gagcagctcg ccgaaatgcc cgcggtcgag ttcgatgctc
     6421 acgatcggat tcgggatgcg gtgccgtctc tgctgcgcag cgctcaaacg gcgtttcccg
     6481 tcgtgtctgg cagtgaagtt gccggcgtgg tgctgaggca ggacttgatt cgagcggcgg
     6541 agcagccgga aac
//
LOCUS       DASTKU010000529        11009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1442533, whole genome shotgun sequence.
ACCESSION   DASTKU010000529 DASTKU010000000
VERSION     DASTKU010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11009
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442533"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..888)
                     /gene="flgF"
                     /locus_tag="VFQ61_18260"
     CDS             complement(145..888)
                     /gene="flgF"
                     /locus_tag="VFQ61_18260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HET9956458.1"
                     /translation="MSTGIWSAASGAVGQTAALDVAANNVANATTPGYRADAAVFRQT
                     LANAVGRSVGSTSQRYAITRTTSPDMRMGQLVHTGRSLDVAISDDRGFFAVATPDGER
                     YTRAGSFRMRTDGTLVTAEGFSVLGSNRRPIQLPAAAGAPGVAADGSIHVNGEPTGTK
                     IGLFTFTNLSGLEKSGQVMFRARPEAGAPQPHDAVLEAGTLEQSNANAVSSMTTLVNA
                     SRQFEMMARVIEAFSQIDRRAATDIMGRT"
     gene            1251..1562
                     /locus_tag="VFQ61_18265"
     CDS             1251..1562
                     /locus_tag="VFQ61_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956459.1"
                     /translation="MRFTSGSTRPGPADCRVRPEHLKDLSKIQALLTALNDPYANIAK
                     LVVAIPVLAARCIVRAAARSKLDIATVDQALNLIGNKGLESELLVLLEDLTILKAETE
                     A"
     gene            1642..2442
                     /locus_tag="VFQ61_18270"
     CDS             1642..2442
                     /locus_tag="VFQ61_18270"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HET9956460.1"
                     /translation="MAGLPAEANREPSVGSAEIRGTEAARAFEQALERVRDKPSATLV
                     APVRTKLDTAQASQALRQAWVRVTGEEPSEDTVRVLTAQWAHETGHGASMFNFNFGGI
                     KGTGPSGLSVAQRTREGYGKNEISIVDRFRAYQTPEEGATDYVRLLSSRFPEATDAAK
                     RGDPVAFVHGLKSRGYFTGDPAAYTRSIVSLSGMSLPSGPVEPFPALATRVQAPPLNA
                     QPAAVRTVMPEALPVMPPLPSVDAMALVDEISRAALRIAAQSKPREVG"
     gene            2439..3128
                     /locus_tag="VFQ61_18275"
     CDS             2439..3128
                     /locus_tag="VFQ61_18275"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9956461.1"
                     /translation="MNAGASTGEERYLDVRYSEATRPFTDYPSRLTRYLAERHLAHLS
                     GGALLDLGSGRGEFLHGFGELGFRAQGIDRARPTSTRFPEPVVQADYERDGLPFADGS
                     FDVLFNKSVFEHITDIGKVLRECLRVIRPGGRMISLVPDWQAQWRHFYDDWTHVRPFT
                     LTGLRECVQSHGFEVREAARFRQLPWLWERPYLQPIASLAALLPVPLKRFKVVRFSKE
                     WMLLVVADRPV"
     gene            3181..4047
                     /locus_tag="VFQ61_18280"
     CDS             3181..4047
                     /locus_tag="VFQ61_18280"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HET9956462.1"
                     /translation="MSVVKIGDVRVGDGEPCYVVGEIGINHNGDLGLARRLVDVAAIA
                     GAQAVKFQKRTVDVVYTADELARPRESPFGQTNGDLKRGLEFGLDEYRNLDRYCREKN
                     VAWFASCWDEASVDFIEQFAPPAYKIASASLTDDALLRHHRATRRPIILSTGMSTQAQ
                     VDHAVNVLGTEDLILLHTTSAYPAKISDLNLRMIPALRERYGIPVGYSGHEVGLASSY
                     AAVALGACMIERHITLDRAMWGSDQAASVEPQGLMKLVRDIRVIESSLGDGVKRVTED
                     EVPIQKKLRRVG"
     gene            4120..5013
                     /locus_tag="VFQ61_18285"
     CDS             4120..5013
                     /locus_tag="VFQ61_18285"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET9956463.1"
                     /translation="MPRSYPRIGIMQGRLSPRPADRLQAFPHHTWRDEFTRAKALGFD
                     AIEWIFEADRHQENPISTAVGRARIRAVVGESGLPVGSVCADYFMVRRLAGESESTRR
                     ENTDVLRELIAWTRELGASRILLPLLETSAVETEELKAQAVASLRDVAKAAAHHGVVL
                     GLETELPGDEYRELVTRVGHPNVRAYYDVGNSTAQGRDVAIDVMPLLGELYAVHLKDR
                     KRLGGSVFIGTGDTNFLGFFGLLKQCGFAGDMVLQHFFETEPEATAARALSTIRNIWQ
                     AARTPLPEVTSALDQSVLEQR"
     gene            5014..6090
                     /locus_tag="VFQ61_18290"
     CDS             5014..6090
                     /locus_tag="VFQ61_18290"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9956464.1"
                     /translation="MTAIWQPGTPRSIRRVLFAGLGGVGQRHLRNVRALLGEEVRVDA
                     YRVRRESWVLDDQLRVVPGAELEQRYALEVYDDLERALAQAPDVVFVTNPSSLHVEVA
                     LRAARANADVFIEKPLSHSLEAVADLERELSRRGLCSYVGYQLRNHPGFRRLRETLTD
                     ASLGPVLSVRAEVGEYLPGFHPYEDYRRMYAARSNLGGGVTLSQIHEIDCLVALFGLP
                     KRGFALGGKVSSLEVDVDDLCSGLLEFEYRGRRLIAELHQDFFQRPTSRHFSVVGERG
                     RAEWSLSERYFRRWDAQGELQDQVSYAEFPRNQMFIDELSEFFECVRTRRPSPLDVAE
                     GGKSLRVALGLLESQRTFRGVSWS"
     gene            6087..6854
                     /locus_tag="VFQ61_18295"
     CDS             6087..6854
                     /locus_tag="VFQ61_18295"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HET9956465.1"
                     /translation="MTTRPSKPEVLALIPARGGSKSVPRKNLLGIAGKPLIAYSIEHA
                     LGTSAITRTIVSTDDPEIARVAGEFGAEVPFLRPAEFATDTATDLQVFRHALQWLERE
                     QGYRPELVVHLRPTGPIRETARIARAVQGMLDQPEADALRSVSLAEQTPYKMWKIQAG
                     RLAPLIPLPEFPEAHSMPRQGLPKAYWQNGYVDVVRPRTVLELDSMVGRVVLPFVIEG
                     EIHELDYPDQIPALERAVAAFTRGELPLTASGERHSS"
     gene            6851..7687
                     /locus_tag="VFQ61_18300"
     CDS             6851..7687
                     /locus_tag="VFQ61_18300"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9956466.1"
                     /translation="MTQHNTILSSFDLTGRVFVITGGAGLLGIEHALAIAEAGGTPVL
                     ADVRGDAADAAAREVEARTGKRAVPLELDVTSKAHCEQALASVLGRCERVDGLINNAA
                     RNPKVEGAGLSATRFEQFSLEAWQADIDVGLTGAFLASQVFGAHFARQRRGVIVNIAS
                     DLGLIAPDQRIYRKPGLADDEQPVKPVTYSVVKGGLVMLTRYLATYYADRGVRVNALC
                     PGGVQNQQPSDFVERLTALIPMGRMAKRDEYRAALVFLCSDASSYMTGQNLVIDGGRT
                     CW"
     gene            7678..8769
                     /locus_tag="VFQ61_18305"
     CDS             7678..8769
                     /locus_tag="VFQ61_18305"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET9956467.1"
                     /translation="MLVEGLQVLALDVDGVITDGKVRLSAAGEETKDIALRDLDAVTL
                     ARRSGVRVALVTGEDSPLVQAIARRFKVDCVVAGAKDKRVGLIQLCDTMAVSLEQVCF
                     VGDADRDAAAFSSVGLALAPNDASALARRKAHRVLEARGGAGAVAEAVEIVLARRAEA
                     LDRRSFEGKLKQIVEDSIRAHQALLSESLASVSEIAAVITQSLRQGGKILLCGNGGSA
                     ADAQHVAAELVGRFAIEREPFPALALTTDTSILTAVGNDWDFRDVFARQVRALAKPHD
                     VVVGISTSGRSANVVRALEAAREKGARSLAFVGRNGGAVAKAAELCFRAPDTNTPRIQ
                     ELHILAWHGICEVVEAALASPRTLAETGS"
     gene            8771..9766
                     /locus_tag="VFQ61_18310"
     CDS             8771..9766
                     /locus_tag="VFQ61_18310"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9956468.1"
                     /translation="MANPNVQSSKVRGPGDPAASSRRGVLADNVIEELWFGDEDPKRS
                     QLEASRSMAAALGIATGLRPFPVVLQKALALLRDPDAPRRKVVELIEQDPALTSRLLR
                     VANSALYGGARTCHSVMDAVMRLGNRTVSDLITGIAVLGMFADVGGYGARVRDHSAAV
                     AAIARVLAIEWRHSGTDHVFLAGLMHDIGKLLMVQAGTPRYEQIPKAVLAQADRVHVE
                     ERHLVGYDHATLGAHVLSHWQLPVEVSRVVAWHHRPGRAFEEGGEVALDVALVRLSDH
                     IELAAASAHKPDAALWEQIGKEEATQYAGFDVAAVEAMWPKLLQARTEALSALTR"
     gene            complement(9769..10503)
                     /locus_tag="VFQ61_18315"
     CDS             complement(9769..10503)
                     /locus_tag="VFQ61_18315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956469.1"
                     /translation="MALPSLVACGGGNPPAATPATTTNAVAAPSASASAPTVEPPKAV
                     NAGQCLATPLLDDAEDGDNKGKVADQRGGYWYTFADSQGSSVEPKGNFQMSKPGASGS
                     QYAARMHGKMGPSGILYAGMGFSFTDPKAPYDAACCKGLRFMAKKDGKGTGNVRLKVG
                     DTNTTPEGGVCKSCYNDFGADLALTNDWKQYSFAFADMKQEPYWGEPQPKIESGRLMQ
                     VQWQVKEPGQEFDIWVDQVEFFDCGK"
     gene            complement(10751..>11009)
                     /locus_tag="VFQ61_18320"
     CDS             complement(10751..>11009)
                     /locus_tag="VFQ61_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956470.1"
                     /translation="PLPHARSNGALYWLIFRKGTTIGELPLSPREVRLFESLPAAPLG
                     QALAAIEATCPEQDRANLPAWLQHWLGRSMELEMWTTVKLE"
BASE COUNT         1934 a         3407 c         3602 g         2066 t
ORIGIN      
        1 gtcgggcgtt tcgacgccac acgtcacgcc ccgggatgat gtgtgggtcc ccaaagcact
       61 tgagatcgcg cagtccgcag cgggcacccg aggtcggccc ccctaaaatg tatccgagta
      121 gtgagctcgc aggagacgca cacctcaggt gcgtcccatg atatccgttg ctgctctgcg
      181 atcgatttgg ctaaaagcct cgatgactcg cgccatcatc tcgaattgac gtgaggcgtt
      241 caccagtgtg gtcatagaag agactgcatt ggcgttggat tgctcgagcg ttcctgcttc
      301 gagcacggcg tcgtgcggtt ggggggcacc ggcctcgggc cttgctcgaa acatcacttg
      361 accactcttt tcgagcccgc tcaggttggt gaaggtgaaa agccctatct tggtgcccgt
      421 gggttcgcca ttgacgtgaa tgcttccgtc cgcggccact cctggcgctc ccgcagcggc
      481 gggtagctga atggggcggc gattcgatcc gagcacgctg aagccttctg ctgtgaccag
      541 cgtgccgtcg gtgcgcattc gaaagctgcc tgcgcgcgtg taacgttcac cgtccggcgt
      601 tgccacggcg aagaagccgc gatcgtcgga gatggccaca tctagactgc ggccggtatg
      661 cacgagctga cccattcgca tgtccggtga ggtcgtgcgg gtgatggcgt acctctgtga
      721 agtggaacct accgagcggc ccacggcgtt ggctagagtt tggcgaaaga cggcagcgtc
      781 tgcgcgataa cctggagtgg tggcgttggc gacgttattc gctgcgacgt ccagcgctgc
      841 cgtttgtccg acggcgcctg acgctgctga ccagataccg gtgctcatgc gttgttctcg
      901 tgctaactcg ggcccgtact cgagctggtc cagagcaagc cttgcgccag tatccgtgtt
      961 gcgcgaaaac ctgcggcaag cgccacttgg acaggcggcg ttgacggcgt tcgccagcga
     1021 tctgggggac ccgcgtacat gccactccca ccgccagcgc cttttcgcag ggctgggcgg
     1081 tcgagctcac gtcattcagc tgacaggttg accgtgtcag ctcgatgact gatcgacctg
     1141 ctcttgcccg tagtggacag agcgcttgct cgtcacccac acccggaatc ctcccccctt
     1201 atgaatcgag gcttgcgggt agcgcccgat ctcgcacaat ctacaggtcg atgcggttca
     1261 cgtctggctc tacccggccc ggtcccgcag actgccgggt ccgccccgaa catttgaagg
     1321 atctgagtaa gatccaagct ttattgaccg ctctgaacga tccttacgcg aacatcgcga
     1381 agctcgtcgt ggcgataccg gtcttggcgg cgcgttgcat cgtgcgggcc gcggctcgtt
     1441 ccaagttgga tatcgcgacc gtggatcagg cgctcaatct cattggcaat aagggcttgg
     1501 agtcggagct cctggtgctg ctcgaagatc tcaccattct caaagccgag accgaggcct
     1561 gagcggcgct attctcgagg cagttggtag tggcccgctt cttgccgttt tccgggcgtg
     1621 cctctcgcga ttactccttc tttggctggc ctcccggcag aggcgaatcg tgaaccaagt
     1681 gtgggctccg cggagatacg cggcaccgaa gcggctcgcg ccttcgagca agcactagag
     1741 cgagtacgcg acaagccgag cgcgacgctg gtcgcgcccg ttcgcaccaa gctcgatacg
     1801 gcgcaggcaa gccaggcgtt gcgtcaagcg tgggttcgcg tcaccggaga ggagccgagt
     1861 gaagacaccg tgcgcgtgct aacggcgcag tgggcacacg agacggggca tggcgcctcg
     1921 atgttcaact tcaactttgg cggcatcaaa gggacggggc ccagcggtct gagcgtcgcg
     1981 cagcgaacgc gagaaggcta cgggaagaac gagatcagca tcgtggatcg ctttcgggcc
     2041 tatcagactc ccgaagaggg agcgaccgac tacgtgcgtc tcctgagctc gcgttttccc
     2101 gaagccacgg atgccgccaa gcgcggcgat ccggtcgcct tcgtgcacgg cttgaagtcg
     2161 cgcggatact tcacgggaga tcccgcagcg tacacgcgta gcatcgtttc gctctcgggc
     2221 atgagccttc ccagtgggcc ggtggagccg tttccggcgt tggccacgcg cgtgcaagca
     2281 ccgcccttga atgcgcaacc ggcagccgtt cgcacggtga tgcccgaagc gttgcccgtc
     2341 atgcccccgc tgccgagcgt ggatgccatg gcgttggtcg acgaaatcag tcgagcagcc
     2401 ctgcgcatag cggctcaaag caaacctcgg gaggtcggat gaacgccgga gcatcgacag
     2461 gcgaagaacg ctacttggac gtccgctact ccgaggcgac ccgtcccttc acggactacc
     2521 cgtctcgact gacgcgctac ctcgcagagc gacacctagc gcacctgagc ggcggcgcgc
     2581 tactggattt ggggtccggg cgcggtgagt tcctgcacgg cttcggtgaa ctcgggtttc
     2641 gcgcccaagg catcgatcgc gcgcgcccca cgagcacgcg gtttccggag ccagtggtgc
     2701 aagccgacta cgagcgtgac ggcttgccct tcgcggatgg cagcttcgac gtgctgttca
     2761 acaagtccgt gttcgagcac atcacggaca tcggcaaggt tttgcgcgag tgcctgcgcg
     2821 tgatccgtcc cggcggacgc atgatctcgc tcgttccgga ctggcaagct cagtggcgcc
     2881 acttttacga cgattggacc cacgtgcggc cgttcacgct cacgggccta cgcgagtgtg
     2941 tccaaagtca cggcttcgag gtgcgcgaag ctgcccgctt ccgccagctg ccgtggttgt
     3001 gggaacggcc ctatctccag cccatcgcgt cgttggccgc gttgctcccc gtgccgctga
     3061 agcgcttcaa agtcgtcaga ttttccaagg aatggatgct gctcgtggtg gccgacaggc
     3121 ctgtgtgagc tctgcccgcg cagcaaagaa ccaacacgag ccaaggatgg aatcgagacc
     3181 atgtctgtag tgaagatagg tgatgttcgc gtaggtgacg gtgagccctg ttacgtcgtc
     3241 ggtgagatcg ggatcaatca caacggagat ctggggctgg cgcggcgctt ggtcgacgtg
     3301 gcggccatcg ccggagccca ggcggtgaag ttccaaaagc gcacggtgga cgtggtctac
     3361 acggcggacg agctggcgcg tccgcgcgag agcccattcg ggcaaaccaa cggcgatttg
     3421 aagcgagggc tcgagttcgg attggacgag tatcggaatc tcgaccgtta ctgccgagag
     3481 aagaatgtcg cctggttcgc gtcatgttgg gacgaggcct cggtcgattt catcgagcag
     3541 tttgcgccgc ccgcttacaa gatcgcgtcc gccagcctga ctgacgacgc cttgctcagg
     3601 caccatcgcg ccacgcgccg accaatcatc ctgtctacgg gtatgagtac tcaggctcag
     3661 gtcgatcacg cggtgaacgt tctcggtacc gaagatctca tcctgttgca caccaccagc
     3721 gcgtatcccg ccaaaatctc cgatctgaac ctgcgcatga tccccgcatt gcgcgaacgt
     3781 tatggcatcc ccgtgggcta ctccgggcac gaggtcggcc tggcctcgag ctacgccgcg
     3841 gtggccttgg gcgcttgcat gatcgagcga cacatcaccc tggaccgggc aatgtggggc
     3901 agcgatcagg ccgccagcgt ggagccgcag ggcttgatga agttggtccg cgacattcgc
     3961 gtgatcgaat cgagcctcgg ggacggagtc aagcgcgtca ccgaggacga agttcccatt
     4021 caaaagaagc tgcgccgcgt cggctgaaac cgtcaccgcg cgtcgagcca acgcttcgaa
     4081 ccaagccagc agacaaaaac gagcagagtg ggtcaagaca tgccgcgaag ttatcctcgc
     4141 atcgggatca tgcaagggcg tctttcgcca agaccagccg atcgactgca agcctttcct
     4201 catcacactt ggcgcgacga gttcacgcgc gccaaggcgc tcggcttcga cgcgatcgaa
     4261 tggattttcg aagccgaccg ccatcaagaa aacccgatct cgactgccgt cggccgggct
     4321 cggattcgag ccgtcgtcgg ggagagcggg ctcccggttg gctccgtctg tgccgactac
     4381 ttcatggtgc ggcgcctggc gggggagagc gagtccacgc gccgtgagaa cactgacgtg
     4441 ctgcgcgagc tgatcgcatg gacccgagag ctgggcgcat cccggatctt gctgcctctg
     4501 ctcgagacct cggcggtaga gaccgaagag ctcaaggcgc aggccgtcgc cagcctgcgc
     4561 gacgtggcca aggccgccgc ccaccatggg gtcgtgttgg gcttggagac agagctaccg
     4621 ggcgacgagt atcgcgaact cgtgacgcgc gtgggtcacc cgaacgtgcg cgcctactac
     4681 gacgtcggca actcgaccgc ccaggggcga gacgttgcaa tcgacgtgat gccactgctc
     4741 ggtgagctct acgccgttca cctcaaagac cgcaaacgct tgggtggcag cgtcttcatc
     4801 ggcaccggag acaccaactt cctcgggttc ttcgggcttc tcaagcagtg cggctttgcc
     4861 ggagacatgg tgctgcagca cttcttcgaa accgagcccg aggcgaccgc ggcgcgggcg
     4921 ctgtcgacca ttcgcaatat ctggcaagcc gcgcgcacgc ccttgcccga ggtgacttcg
     4981 gcgctcgacc aatccgtgct ggagcagcgc tgaatgaccg ccatttggca gccgggcacg
     5041 ccgcgttcga ttcggcgcgt cttgtttgcg ggcttgggcg gagtcggcca gcgtcatctg
     5101 cgtaatgttc gggcgctgct cggagaagag gtgcgcgtcg atgcttaccg ggtccgtcgc
     5161 gagagctggg tgctcgacga tcagcttcgg gtggtgcccg gggcggagct cgaacagcga
     5221 tatgcgctcg aagtctatga cgacctcgag cgtgcgctcg cgcaagcgcc agacgtcgtc
     5281 ttcgtcacca acccttccag cttgcacgtc gaggtggcat tgcgcgcggc gcgcgcgaac
     5341 gccgacgttt tcatcgaaaa accactgtct cactcgctcg aagcggtggc cgacttggag
     5401 cgagagctga gccggcgcgg actatgtagt tacgtcggct atcaacttcg aaaccatccc
     5461 gggttccgcc gtttgcgtga aacgttgact gacgcgagcc tcggtcccgt gctcagcgtg
     5521 agagcagagg tcggcgagta cctgcccggg tttcatccct acgaggacta ccgtcgcatg
     5581 tacgcggcgc gcagcaacct gggcggtggg gtcacgctct ctcagattca cgagatcgac
     5641 tgcttggtcg cgttgttcgg gttacccaag cgtggcttcg cgctcggggg caaagtctcc
     5701 tcgctcgaag tcgacgtgga cgacctgtgc tccggcttgc tggagttcga atatcgcggg
     5761 cggcgcctga tcgccgagct tcatcaggac tttttccagc gcccgacgag tcgtcacttc
     5821 agcgtcgtgg gcgaacgcgg gcgagcagag tggagcctga gcgagcgcta cttccggcgc
     5881 tgggacgctc agggggagct gcaagatcag gtctcgtacg cagagttccc gcgcaaccag
     5941 atgttcatcg acgagcttag cgagtttttc gaatgcgtgc gtacacgccg gccgagcccg
     6001 ctcgacgtgg cggagggtgg taagagcttg cgcgtcgcgc tcgggctact cgagtcacag
     6061 cgcacattcc ggggagttag ctggtcatga ccacacgtcc gtccaagccc gaggtgctcg
     6121 cgttgattcc cgcgcggggt gggtcgaagt cggtgccccg caagaacctg ctcggcatcg
     6181 cgggcaagcc gctgatcgca tactcaatcg agcacgcgct cggaacatct gccatcacgc
     6241 gcacgatcgt atctactgac gatcccgaga tcgcgcgcgt ggccggggaa ttcggggccg
     6301 aggtaccgtt cctccggccc gccgagttcg ccaccgacac cgcgacggac ttgcaagttt
     6361 tccggcatgc tctgcagtgg ctggagcgag agcagggcta tcgccccgag ctcgtcgtgc
     6421 acctgcgtcc caccggaccg attcgagaaa cggcccgcat cgcgcgcgcg gtgcaaggga
     6481 tgctggacca acccgaagcg gatgcgctgc gctcggtgag cctggctgag cagacgcctt
     6541 acaagatgtg gaagattcag gcgggtcgcc tcgccccgct catcccgcta cccgagtttc
     6601 ccgaggctca ctcgatgcct cggcagggtt tacccaaggc atactggcag aacggttacg
     6661 tcgacgtggt gcggcctcgc accgtgctcg aactcgactc gatggtgggg cgagtcgtct
     6721 taccgttcgt aatcgagggc gagatccacg agctcgacta cccggatcag atccctgcgc
     6781 tcgagcgcgc cgtcgccgcg ttcacccgtg gtgagctgcc gctcacggca agcggcgaaa
     6841 ggcattcgtc atgactcagc acaacacgat tttgagcagt ttcgacctga ccgggcgcgt
     6901 cttcgtgatc accggtggag cgggtctact cggcatcgag cacgcgctgg cgatcgcgga
     6961 agcgggcggg accccggtgc tcgccgacgt acggggagac gccgccgacg cggccgcgcg
     7021 cgaggtggaa gcccgcactg gcaaacgtgc cgtccccctc gagctggacg tcacttccaa
     7081 ggcgcactgc gagcaagctc tcgcgagcgt gctcggacgc tgcgagcgtg tcgacggatt
     7141 gatcaacaac gcggcccgca atcccaaggt cgaaggggcg gggttgtccg ccacgcgctt
     7201 cgaacagttc tcgctcgagg cctggcaagc cgacatcgac gtaggtttga cgggagcgtt
     7261 cctggcaagt caggtttttg gagcccactt tgcccgacaa cggcgcggag tcatcgtgaa
     7321 catcgcgtct gacttggggc tgattgctcc agatcagcgc atttatcgca agccggggtt
     7381 ggcggatgac gaacagcccg ttaagcccgt cacttactcg gtcgtcaagg gagggttagt
     7441 gatgctcacg cgttacctcg cgacctatta cgctgaccgc ggggtgcgag tgaatgcgtt
     7501 gtgccccggg ggagtgcaaa accagcagcc gtcggatttc gtcgagcgcc tcaccgccct
     7561 gattcccatg gggcgcatgg cgaaacgtga cgagtaccgc gcagcgctcg tgtttctttg
     7621 ctcggatgcc tcctcttaca tgactggtca aaacctcgta atcgatggag ggcggacgtg
     7681 ttggtagagg gtttgcaagt tttggcgctc gacgtcgacg gtgtgattac ggacggcaag
     7741 gtgcgcctct cggcggctgg tgaggagacc aaggacatcg ccctgcgcga tctcgacgca
     7801 gtgacgctgg ctcggcggag cggcgtgcgg gtggccctcg tgaccggcga ggattctccg
     7861 ctggtgcaag ccatcgcgcg ccgcttcaag gtggactgcg tggttgcggg agccaaggac
     7921 aagcgcgtcg gactcatcca gttgtgtgac acgatggcgg tgtcgctcga gcaagtttgc
     7981 ttcgtggggg acgcagaccg tgatgcggcc gccttctcga gcgtcggcct cgcgttggcg
     8041 ccgaacgatg cctctgcgct cgcacgccgc aaggcccacc gcgtactcga agctcgtggc
     8101 ggcgcgggcg ccgtcgcgga ggcggtagaa atcgtactcg cgcggcgcgc cgaagctctc
     8161 gaccgacgat cgttcgaagg caagctgaag cagattgtag aggatagcat tcgagcgcac
     8221 caagctctgc tttcggaatc gctggctagc gtctcggaga tcgccgcggt catcacgcag
     8281 agcttgcgtc agggaggcaa gatcttgctg tgcggcaacg gcggtagcgc cgccgacgcg
     8341 cagcacgtgg cggccgagtt ggtcgggcgc tttgccatcg agcgcgagcc ctttccggcg
     8401 ctggctctga ccaccgacac ttcgatcttg accgccgtgg gtaatgattg ggactttcgc
     8461 gacgtgttcg cgcgccaggt gcgcgcgctc gccaagcccc acgacgtggt cgtaggcatc
     8521 agtacgagtg ggaggtccgc gaacgtcgtg agagcgctgg aagcggcgcg tgaaaagggc
     8581 gcgcgcagct tggctttcgt ggggcgaaac ggtggcgcgg tggccaaggc cgcggagcta
     8641 tgcttccgcg cgccagacac caacacccca cgcattcaag agctacacat cctcgcttgg
     8701 cacggtatct gtgaggtggt cgaggcagcg ttggccagcc cgagaacgtt ggctgaaaca
     8761 gggagttgat atggcaaatc cgaacgtgca gagctccaag gtccgcggcc caggcgatcc
     8821 agcggcgagt agtcgtcggg gggtgctcgc ggacaacgtg atcgaagagc tctggttcgg
     8881 agacgaggac ccgaagcgca gccagctcga ggcgtcacga tcgatggcgg cagcactcgg
     8941 gatcgcgacc gggctgcgac cgtttcccgt ggttttgcag aaggcactgg ctctgctccg
     9001 cgacccagac gcaccacgcc gcaaggtggt cgaactcatc gagcaggatc cagcgctcac
     9061 atcgcgcctg ctgcgcgtcg caaactccgc tctgtatggt ggcgcccgca cctgtcacag
     9121 cgtgatggac gcggtgatgc gactcggcaa ccgcaccgtg tccgacctga ttactggaat
     9181 cgcagtgctc ggaatgtttg ccgacgtcgg tggctacggc gctcgagtcc gagatcactc
     9241 ggcggcggtg gctgcgatcg cacgcgtgct tgccatcgag tggcggcaca gcggcacgga
     9301 tcacgttttc ttggcgggtc tgatgcacga catcggcaag ctactgatgg ttcaagccgg
     9361 cacgcctcgt tacgagcaga ttccaaaggc cgtactcgcc caagccgacc gcgtccatgt
     9421 cgaggaacgg cacttggtcg gctatgatca cgccactctt ggggcccatg tcctttccca
     9481 ttggcagctt ccagtggaag tcagccgggt ggtggcgtgg caccaccgtc cgggccgtgc
     9541 cttcgaagaa ggcggcgaag ttgccttgga cgtagccctg gttcggctct ccgatcacat
     9601 cgagttggcg gctgcgtccg cgcacaagcc agacgcagcc ctctgggagc aaatcgggaa
     9661 agaagaggcc acgcagtacg ccgggttcga cgtcgcggcg gtcgaagcga tgtggccgaa
     9721 gcttcttcag gcgcgcacgg aagccttgtc cgcgctgacg cgctgacgct acttgccgca
     9781 gtcgaagaac tcgacctggt cgacccagat gtcgaattcc tggcccggtt ccttgacctg
     9841 ccactgcacc tgcatgaggc gcccactctc gatcttgggt tgcggctcgc cccagtacgg
     9901 ctcctgcttc atgtcggcga acgcaaacga gtactgcttc caatcgttgg tcagcgcgag
     9961 atccgcgccg aagtcgttgt aacagctctt gcacacgccg ccttcgggcg tggtgttggt
    10021 atcaccaacc ttgagccgga cattgccggt tcccttgcca tcctttttcg ccatgaagcg
    10081 gaggcccttg cagcacgcgg catcgtaggg ggctttggga tcggtgaacg agaagcccat
    10141 gcccgcgtac agaatgccgc tcgggcccat tttgccgtgc atgcgcgcgg catattgaga
    10201 gccgctcgcg cccggcttcg acatttggaa gttgcccttg ggctccaccg agctgccctg
    10261 actatcggcg aaggtgtacc agtagccgcc gcgttgatcg gcgaccttgc ccttgttgtc
    10321 tccgtcctct gcatcgtcga gcagcggggt agccaggcat tgaccggcgt tgacggcctt
    10381 gggcggctcg accgtcggcg cgctcgcgct cgcgctcggc gcggcgacgg cgttggtcgt
    10441 agtcgcaggc gttgcggcag gcggattgcc gccgccacag gcaaccaggg aagggagcgc
    10501 cacggcgagc gcgtacattc gggaactcga accactgaga agctggttag tgcgcatcat
    10561 cttgggggtc tggtcttacc gagtggcgtt cgcgatgcaa ggacgacctc tatcgttctc
    10621 gtgcggcagc ctcttcggat caccgcttcg gtgggcctcg ccgggcggtc ctgcggactc
    10681 acaaaggctg aaaacgagaa gcgagtgatt caatgtgacc agtgcgcctt cgagccaagg
    10741 actgagccgt tcactccaac tttacagtgg tccacatttc gagttccatg ctgcgcccga
    10801 gccagtgctg caaccaagcg ggcagattcg ctcggtcctg ttcgggacag gtcgcttcga
    10861 tggctgccag agcttgcccg agcggtgcgg ccggcagcga ttcgaagagc cgcacttctc
    10921 gagggctgag cggtagctcc ccgattgtcg tgcccttgcg aaagatcagc cagtagagcg
    10981 ccccgttcga cctggcatgg ggcagcgga
//
LOCUS       DASTKU010000530        14433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1218696, whole genome shotgun sequence.
ACCESSION   DASTKU010000530 DASTKU010000000
VERSION     DASTKU010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14433
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218696"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4566)
                     /gene="glgX"
                     /locus_tag="VFQ61_18325"
     CDS             complement(<1..4566)
                     /gene="glgX"
                     /locus_tag="VFQ61_18325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET9956471.1"
                     /translation="MNTLPGLPHPLGATWDGHGVNFALYSEHAERVELCLFDDQDNET
                     RIPLPHHTAFVWHGYVPGLQPGQRYGYRVHGPYEPQRGLRFNPNLVLLDPYAKALATT
                     ERWERGCFAYAIGSPEQDLRFAEEPGLGAPRAMVVDPGFDWDGDVPPRTPLHRTVIYE
                     AHVRGLTMRHPALPPALRGTYAGVAHPAVIEHLTQLGVTAIELMPVHAFVDDKHLLDK
                     GLRNYWGYNSIGFFAPDVRYRMGNELGQEVVQFKQMVKALHRAGIEVILDVVYNHTAE
                     GNHLGPTFSFKGIDNPTYYRLVSGDERHYFDYTGTGNTLNVRHPQVLALIMDSLRYWA
                     EEMHVDGFRFDLASALARQLHDVDRLSSFFTLIHDSPTLREVKLIAEPWDVGHGGYQV
                     GNFPVRWAEWNGRYRDTMRSFWKGDGGRAADLGFRLTGSSDLYEASGRKPSASINFIT
                     AHDGATLRDLVSYNHKHNEANGENNADGNNDEHSWNCGAEGPTDDPEILNLRRRQQRN
                     LLATLLLSQGTPMILAGDECGRTQNGNNNAYCQDNEIGWFDWDWGDEQRALFEFTRRV
                     LALRKAHPALHRSKFFQGRPIQNADLSDLAWFRHDGLPMSQEDWQDPSTQSLVMFLAG
                     RGIGDVDDAGRPLVDDNLLLAINASHLDLELAFPDVQRVDQWELILDTTDDRADGTLA
                     ARSKTTLGARSLKLFRAPARVVRSGGALHTLGSTYRLQLHRDFGFRAALEQLDYLAKL
                     GVTDLYLSPIFAAATGSTHGYDVVSHEKLNPELGTLEDFQALAESARTHGMGILVDWV
                     PNHMGNAAGQNAWWEDVLENGPSSLHAVAFDIDWNPVKEELRDTVLLPVLADQFGQVL
                     ERGDLRVELEGGSFFVRHMESRFPAAPKSLLPLISDAVAATGLSELHPAQQELESLRA
                     SLFHLPNRRETSEETRRERAREKEVFKRRLERLMRESPEVAQAVARAVEKLNGTPGEP
                     ASFDALDRFLGEQSYRLASWRVASQEINYRRFFDVNALVALRVEEPVVFERAHRLLFQ
                     LLNDGLIQAIRLDHTDGLFDPLAYFEQLQRHFRGTHPPGAYVTPDDAARPLPILVEKI
                     LEAHEKLPAAWPVDGTTGYEFAAAAIGVSVDASVEPVFTQIYQEFTGDRRSFSEHVYE
                     SKIRILKDSLASEVNMLARRLERIAVRNRAWRDFTLVSLTRALSELLAAFPVYRTYLR
                     LGSKPDVEDERTVTFAIAQAQKNAGADVDPSVFEFIRDLLLSRKDASPEERLAQNNFA
                     LRFQQLTGPVMAKSVEDTAFYRYQRLIALNEVGGDPASFGLSTEAFHAQYMEQLRSWP
                     LGMLTTSTHDTKRGEDAAARLAVLTEMPQPWRDAVREWSALNHKYRTEAERGLAPSRR
                     DEYMYYQALLGAWPFGWDGERGLDSFIQRMQAFMQKATNEAKEETSWIAPSPRYDAAT
                     ASFVERSLSDPEFRKHVRSLGQSIEKPAVTNSLAKVLLRVCGPGVPDTYQGSELWNQS
                     LVDPDNRRPVDYSVRRALLAEIEAEPDR"
     gene            5421..6635
                     /locus_tag="VFQ61_18330"
     CDS             5421..6635
                     /locus_tag="VFQ61_18330"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956472.1"
                     /translation="MRGPLIRFMALSTSTLFIHCSSSGSDAGAGGSTSGAAQSLGGAS
                     TGGVPSSGATKSGGASGGAPTQPIGGNASGGTSSTRGGTASMAGASPAGGTTARGGAT
                     SGGGSSSTGSNNTGGAIGGTSANGGTSANGGTGGMTANGGSAGTTSTGGAGSVTKSAG
                     CGKTPTLTSSGSNNFMPNTLMVSGTSRQYVLRRPQNYDNTRAYRLILDLHGYGGSYSE
                     TAGNYFGLWNLSKDSTIFVALSAPGGDWRTGDNLAYVDEVLKAVEEDTCIDSSRIMLE
                     GFSQGAAMSWSLACSRPGTFRAIVGHSGGGVTNPTSCKPIPYFGSGGLQENVTQTTQS
                     DQFAKWNGCTVAKLPTAKTGSHVCTDYMGCPASAPVRWCSFDGPHTPSPSDSGQSTSW
                     MPQEVWTFLSQF"
     gene            complement(6726..7475)
                     /locus_tag="VFQ61_18335"
     CDS             complement(6726..7475)
                     /locus_tag="VFQ61_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956473.1"
                     /translation="MDTRALVQFKRDPAGFVDSGLFRVMPITAHAPPSPGGIAIDSGE
                     KFSLVRYVPADSLRTLRLKATDLAQNAGHDVICQDGVLDGSADVEGYYLGWKTDEIHM
                     MSLSPLCGADFFVTAKMNGCCVYVSGTQASPTVIHANTGSAALEDFDDSGYATMAEKT
                     TALFEYQATIYRKIYAKLAARLAEFGHIQTGGPVTVFDPGNYRLAGGYDARVFGLRRS
                     GNWTFYYSCERSQNGAETHVTRELWPTFEQI"
     gene            8262..8924
                     /locus_tag="VFQ61_18340"
     CDS             8262..8924
                     /locus_tag="VFQ61_18340"
                     /inference="COORDINATES: protein motif:HMM:NF012671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HET9956474.1"
                     /translation="MPTSGGTPHCGQNTQGKAGSLSWSLWTNTLNNNACMTTYNTTAF
                     SASWNGNGDFLARVGVEFGNSGKTYDQYGLIKADFSYKKTGSAGGYSYIGIYGWSINP
                     CVEWYIVDDRWGTMPFDAYQATPKGTAKIDDENYKLFQNKTPGTGGSRCGNGVTQWDQ
                     FWSIRQKARQCGTITISEHFKAWAAAGLKLGNLLEAKILVETGGGQGSIEFPVANVTV
                     AQ"
     gene            9327..10193
                     /locus_tag="VFQ61_18345"
     CDS             9327..10193
                     /locus_tag="VFQ61_18345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956475.1"
                     /translation="MITRRCVRRQFLLRPDARTNQCFLFCLALAAKRSGVQVVAFLVM
                     SNHYHAIVHDPLGTLPVFLEHLHKLTARALNARWGRWENLWASEQTCATHLVNPDDVL
                     GKVLYTLCNPVTDQLVDRVTDWPGANSFRAMLSGEAIEVERPRDFFREDGTTPEFARL
                     ELVRPPGFEDCDSTQWRAHLSSLVTAIEERTRAERHRTGGKLLGRKAVLRASAFDTPH
                     SSEPRRNLRPAVACSDEQTRVHVLAQLVAFRRAHEAARQRVAAGELDALFPYGTYKMR
                     LMGHRCASVPPA"
     gene            complement(10467..12071)
                     /locus_tag="VFQ61_18350"
     CDS             complement(10467..12071)
                     /locus_tag="VFQ61_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9956476.1"
                     /translation="MMRTKQRWVLGCTLLSSGLALSCGEIIGIKDLRGNSGGAGASGV
                     GTGGVGTGGKTSTGGTYYSDGGVTTTGGRSGGGGSVGTGDTSAQAGDTGSTQGGAPAN
                     GGASSSGGGAGGTRTSGGATNDGGIPGEGGAGGNTVDDGAVRGHVIDFLGHPVPLANV
                     TIGSTTVQTDKDGAFAVDEAPASYDVRLSVRPIINNTQREYTWIYESLSRRDPKLQVY
                     YGVEKQNASNLNLKTKTSYLDDQHKTVMSFGSKHGSVNVEINYAEVNYLSAVWYGPRT
                     IDGNAHYLTWSFTGNQDLPVAYQSYASTPASLANTGAAISVELTDSMFPVSSTQGKVE
                     SLVTASRRNWAFVHFKDNAQIPVVEDWSGSDTFSYLTPGLPDSAITVAASYGDWTRGA
                     YAIAHKMVSSGSTGIELSIPAAPVLSAPTNAKTNVNGKTEFSWTGANAVHCLAITDGF
                     KQAVYVVTTRKRVTIPDLDAVLTGNAEFSWSVEQHNALRTVDDATGADGFQDSFGAYL
                     EEPTLYRANDQGFYASSESRTFTTAP"
     gene            complement(12071..13111)
                     /locus_tag="VFQ61_18355"
     CDS             complement(12071..13111)
                     /locus_tag="VFQ61_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9956477.1"
                     /translation="MRCVPILLTCGLLVLASPPAARAETAASPPASNDKVAAEALFRE
                     GRKLMSEARYAEACSKFEASQRLDPGVGTMLNLAECYEKTGRTASAWAEFREVISAAR
                     EADSREREELARRRADALETKLSRLTISLSTEATQIRGLEVRRDGTVLDSAEYGSAIP
                     VDPGKHTIEATAPGRGKWFKALEIGADGAQEAVTIPSLPEAGETSKSTNVAVGQVTPD
                     RVESSGSSSKKGVAIALGAVGVVGIGVGTYFGLKASSAWSDAKDGCGDYQNCSEGAIR
                     DGDTARHAATASTIAFGVGIVGLASGTVLWFLSGPSNKDAAANPAYPVKPPKAATRIG
                     VGMGSVVVRGNF"
     gene            complement(13212..14267)
                     /locus_tag="VFQ61_18360"
     CDS             complement(13212..14267)
                     /locus_tag="VFQ61_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956478.1"
                     /translation="MVSRRTVGLVCLLAACKPDIEDGASRVTSDRVLAVRSEPAEVTP
                     GKEVRYTALLASPQSGGDPDALDFAHCLARKPITVPGPIALECLADSAEALSSLGRGN
                     QVSGVIPQTACRLFGPNPPAPAPGEPAARAADPDPSGGYYQPVRVHHATTGEYSVGVT
                     RLACGVARATQDQSLEFTRRYRLNQNPELDRVTARIGDVESELAEGLALSVPAGSRVT
                     LRVQWRDCPTQAACGDGACSIYEDDLSCAEDCAEPRGCTGAEPYVYFDPETSRIVERR
                     ESIRVSWFGTAGSFTQDRTGRQAVGLEGLENQKTDSPESTNEWTAPDVPSEARLWLVI
                     RDDRGGTGFASFVLRVE"
BASE COUNT         2741 a         4552 c         4390 g         2750 t
ORIGIN      
        1 gcgatcgggc tcggcctcga tctcggcgag tagggcgcga cgcacggagt agtcgaccgg
       61 gcgacggttg tctgggtcga ccaggctttg attccacaac tcggagcctt gatacgtatc
      121 cggaaccccc ggcccacaca ctcgcagtaa gactttggcg agcgagttgg tgaccgccgg
      181 cttttcgatg ctctggccca agctgcgtac gtgttttctg aactcgggat ctgacagcga
      241 gcgttccacg aagctggctg tcgccgcgtc gtacctgggt gaaggcgcaa tccaagaggt
      301 ctcctctttg gcctcgttgg ttgccttttg catgaacgcc tgcatgcgct ggatgaagct
      361 atcgagcccg cgctctccgt cccaaccaaa gggccaggcg ccgagcaggg cctgatagta
      421 catgtattcg tcgcgccgag atggcgctag cccgcgctcg gcctcggttc tgtacttgtg
      481 attgagagcc gaccactccc tcactgcgtc acgccatggc tggggcatct cggtgagcac
      541 cgccaggcga gcagccgcgt cttcaccgcg cttggtgtcg tgggtcgagg tggtgagcat
      601 gccgagcggc cagctgcgca gctgttccat gtactgcgcg tggaaagcct cggtgctgag
      661 gccgaaactg gcggggtcac cgcccacttc gttcagggcg atcaggcgct gataccggta
      721 gaaggcagta tcctcgacgg acttggccat cacggggcca gtcaactgtt ggaagcgcag
      781 cgcgaagttg ttttgggcca agcgctcttc cgggctggcg tctttgcgcg ataagagcag
      841 atcgcgaatg aactcgaaca cgctgggatc cacgtcggcg cctgcgttct tttgcgcctg
      901 ggcgatcgcg aacgtcaccg tgcgctcgtc ctcgacgtcc ggcttgctgc cgaggcgcag
      961 gtacgttcgg tagacgggaa acgcggcgag tagttcgctc aaggcccgcg tcaaggacac
     1021 caacgtgaag tcccgccaag cccgattacg cacggcaatt cgctccaagc gccgcgccag
     1081 catattcact tcactcgcca gagagtcctt caaaatacga attttgctct cgtacacgtg
     1141 ctcggaaaag gagcgacgat caccggtaaa ctcttggtaa atttgcgtga agaccggctc
     1201 gacggaggca tccacggaga ccccgatggc ggccgccgca aactcgtagc ccgtggtgcc
     1261 gtccacgggc cacgccgccg ggagcttctc gtgagcttct aggatcttct ccacgagaat
     1321 ggggagcggg cgcgcggcgt catcgggagt tacgtaggcg ccgggcgggt gggtgccgcg
     1381 gaagtgacgt tgcagttgtt cgaaatacgc gagcggatcg aacaagccat ccgtatgatc
     1441 gagtcggatc gcctgaatca agccgtcgtt cagtaactgg aacagcagtc gatgggcgcg
     1501 ctcgaagacc actggctctt cgacccgcaa ggctaccagg gcgttcacgt cgaagaagcg
     1561 acgatagttg atctcctgag aggcgacgcg ccaggacgcc aatcggtagc tctgctcgcc
     1621 gaggaagcga tcgagcgcgt cgaaactggc tggttctccg ggagtgccgt tgagcttctc
     1681 gacggctcgc gcaacggctt gggcaacctc cggggattcg cgcatcaggc gctccagccg
     1741 acgcttgaac acctcctttt ctctcgcgcg ctcacgccgc gtctcttcgg acgtctcgcg
     1801 gcgattgggc aagtggaaca gcgatgcccg caaactttcc agctcctgct gggcggggtg
     1861 aagctcactc aacccggtgg cggccactgc gtcgctaatc aacggcagca agctcttcgg
     1921 tgccgccggg aagcggctct ccatatgacg cacgaagaag ctcccgccct cgagctcgac
     1981 gcgcagatcc ccgcgctcga gcacctgacc aaactgatcc gccaggaccg gcagcagcac
     2041 cgtgtcccgc agctcctcct tgaccggatt ccaatcgatg tcgaaggcca ctgcatgtaa
     2101 ggagctcggg ccattctcca gcacgtcttc ccaccaagcg ttctggcccg ccgcgttccc
     2161 catgtggttc gggacccaat ccaccagtat tcccatgccg tgcgtacggg cggattcggc
     2221 gagcgcctgg aagtcctcca gggtaccgag ctcgggattc aacttctcgt ggctgacgac
     2281 gtcataaccg tgagtgctcc cggtagccgc cgcgaagatg ggggagagat acagatccgt
     2341 aactcctagc ttcgctagat aatcgagttg ctcgagtgcc gcgcggaacc cgaagtctcg
     2401 atgcaactga agccggtagg tcgagcccaa ggtgtgtagc gcgcccccgc tgcgcaccac
     2461 gcgggcgggc gctcggaaca gcttcaaaga tcgggcaccc agcgtcgtct tggaccgtgc
     2521 cgcgagggtg ccgtccgctc gatcatccgt ggtatcgagg atcagctccc attgatcgac
     2581 ccgctgcacg tctggaaacg cgagttccaa atctagatgg ctggcattga tggccagtaa
     2641 caggttgtca tcgacgagag gtctccctgc gtcgtccacg tccccaatcc ctcgacctgc
     2701 caaaaacatg accaggctct gcgtcgaagg atcttgccaa tcctcctgag acatcggcag
     2761 cccatcgtgc cgaaaccaag ccaagtcact cagatcggcg ttctggatag gtcgaccctg
     2821 gaagaacttg gagcggtgca acgcggggtg ggctttgcgc aacgccaaca cgcgccgggt
     2881 aaactcgaac agggcgcgtt gctcatcacc ccagtcccaa tcgaaccatc cgatctcgtt
     2941 gtcctggcaa taagcattgt tattgccgtt ctgcgtgcgg ccacactcgt ctccggccag
     3001 gatcatcggg gtaccctggg agagcagcaa cgtggcgagc aggtttcgtt gctggcgacg
     3061 acgaagattc aatatctcag gatcgtcggt ggggccctcg gcaccgcagt tccaagagtg
     3121 ctcgtcgttg ttaccgtcgg cgttgttttc tccgttggcc tcgttgtgtt tatgattgta
     3181 gctgactaaa tccctcagcg tcgcgccgtc atgggcagtg atgaagttga tgctggccga
     3241 aggcttgcgc ccgctcgcct cgtacaagtc gctgctgccg gtcaggcgaa agccgagatc
     3301 ggcggcgcgc ccgccgtccc ctttccagaa cgaacgcatc gtatcacgat agcggccgtt
     3361 ccactccgcc cagcgcacgg ggaaattgcc gacttgataa ccgccgtgtc caacgtccca
     3421 cggctcggcg atcaacttga cctcacgcag tgtcggcgag tcgtggatca aggtaaagaa
     3481 gctcgacaag cgatctacgt cgtggagttg gcgagccaac gcggacgcca gatcgaacct
     3541 gaagccgtcc acgtgcattt cctcggccca atagcgcaga gaatccatga ttagcgcgag
     3601 cacctgcggg tggcgcacat tcaaggtatt gcccgtgccg gtgtagtcga aatagtggcg
     3661 ctcgtctccc gacaccaagc ggtagtacgt aggattgtcg atgcccttga acgagaacgt
     3721 gggccccaga tggttgccct ccgccgtgtg gttgtacacg acgtcgagaa tcacttcgat
     3781 gcccgcccgg tgcagggcct tcaccatctg cttgaactga acgacctctt gtccgagctc
     3841 gttgcccatt cgataacgca cgtcgggcgc aaagaagccg atcgagttgt agccccaata
     3901 attgcgaagg cccttgtcga gcaggtgctt gtcgtccacg aaggcgtgta ccggcatcag
     3961 ctcgatcgcg gtgaccccca gctgggtgag gtgctcgatg accgctgggt gagcgacgcc
     4021 ggcgtacgta ccgcgcagag ccggcggcaa tgccgggtgc cgcatcgtca gtccgcgcac
     4081 gtgcgcttcg taaatcaccg tccgatgtaa gggcgtgcgg ggtggcacgt cgccatccca
     4141 atcgaagccc ggatccacga ccatggcgcg cggcgcaccg agccccggtt cttccgcgaa
     4201 ccgcaagtct tgctccggac taccgatcgc gtacgcgaaa caaccgcgct cccatcgttc
     4261 ggtcgtcgcc agcgccttgg catatggatc gagcaaaacc agattgggat tgaagcgcag
     4321 accgcgctgc ggctcatagg ggccgtgcac tcgataaccg tagcgttgcc ccggctgcag
     4381 ccccggcacg taaccgtgcc aaacgaacgc agtgtggtgc ggcagcggaa tgcgtgtttc
     4441 attgtcctgg tcatcgaaca ggcagagctc gacgcgctcg gcatgctctg aatacagagc
     4501 gaagttgacg ccgtggccgt cccaagttgc tccgagcgga tgcggtaaac cgggtaaagt
     4561 gttcatggtc ctgcgcagtc gaggtaaaag tcgcagcaaa gctgctcact ctcggtgtcc
     4621 cccatctagc ccaagggagg caacccctta agcgccgtca ttccgcgtcg aacgtcgaat
     4681 tgaatgctta ggttcgactg ccggtgcgcc gcccagcgta ggctccgcct cgctcagggt
     4741 tccatgggtg aatgcaccca atgaaagcgg atttccgcgc acgtctcacc tgtaacgctt
     4801 tgggaaccgt ttggaaagaa gagcgagacc tcagcgcgcg tgccaccagg agagttcacc
     4861 tcggacgctg ggacagatac cgaatcgaga cgggctcacg agatggcgta actcaatgtt
     4921 ggccagctct gattgggagc gaccacgaac tgccattggc gatggctacc tcgaatccat
     4981 gaatcccgac gggattcagg agtgcagtca tcggtccatt cgtcgagctc cgcgcatcac
     5041 tttcaatgtt caggatcttc aagcgatgca cctgcgggcg gcgcattggc agttgcgacg
     5101 cgaccagatt tctgaagtac tcgctgcgcc agtcggttag gaccgctagc gatctgaact
     5161 cgaccgcgaa agatcccgac cgaaccgagg cgctggcgcc ttcaataatg cgtgggccaa
     5221 cggtgattcc gactgacgga atgcacgtaa tccgtccact gcactctcgt gcggccttca
     5281 ttcgaatgct ctttacgtgg aacggtgaac ccccgttcaa agtttgccgc agcgacggag
     5341 cacgcacaag cgccgcggag aaaccgcagc gcgagaactg gcaataaggc gcgtgttttt
     5401 ttgaacacct aggagttgtc gtgcgaggac cactcattcg attcatggcg ctgtccacca
     5461 gcacgctgtt cattcactgt agcagctcgg gaagtgacgc cggagcgggc ggctcgacga
     5521 gtggagctgc acagtccctc ggtggagcga gcacgggcgg ggttccgagt tccggcgcca
     5581 caaagtccgg cggtgcgtcg ggcggagcgc caacccaacc catcggtggc aacgcgagtg
     5641 gcggaacgtc gagtacccgt ggcggcaccg catcaatggc gggagcctcc ccagctggcg
     5701 gcactacggc gcgcggaggc gccacgagcg gaggcggctc cagctcaacc gggagcaaca
     5761 acaccggtgg agccattggc ggcacctcag caaacggcgg cacctcagca aacggcggca
     5821 ctggcggcat gacggcgaac ggaggcagcg ctggcaccac gtccaccggc ggcgctggca
     5881 gcgtaacgaa gagcgcaggc tgcggcaaga ccccgactct caccagcagt ggcagcaaca
     5941 acttcatgcc caatacgctc atggtgtctg gtacgagtcg ccagtacgtg ctgcggcgcc
     6001 cgcagaacta cgacaatacc cgagcatacc ggttaattct cgatttgcac ggttacggtg
     6061 gttcgtactc cgagacggcg ggcaactact tcggactctg gaatctctcg aaagacagca
     6121 cgatcttcgt cgcactttcg gcgccgggcg gagactggag aactggcgat aacctcgcat
     6181 atgtggatga ggtattgaaa gccgtagaag aagacacgtg tatcgattcg tcgcgaatca
     6241 tgctcgaggg gttcagccag ggcgccgcca tgtcgtggtc gctagcctgc tcacggccgg
     6301 gcactttccg cgccatagtc ggccattccg gtggcggagt gaccaacccg acgagttgca
     6361 aacccatccc ctacttcgga tcgggtggcc tccaggagaa cgtcacgcaa actactcaga
     6421 gcgaccaatt cgccaagtgg aatggctgca cggtagcgaa gctgcccacc gccaagacgg
     6481 gcagccacgt ttgcacggac tacatgggtt gcccggcctc cgcgcccgta cgctggtgtt
     6541 ccttcgacgg cccccacacc ccctccccct ccgattcagg ccagagcacg tcttggatgc
     6601 cgcaagaggt ctggaccttt ctcagtcaat tctaggcacc ttgggcgcag catggatttc
     6661 tagaggtgac gctaacgctt tcgtgaacac cgtatgcttc gtttcctgca gatgccagag
     6721 gcccgctaga tctgttcgaa agtgggccag agctcgcggg tgacgtgggt ttcggcgccg
     6781 ttctgggagc gttcgcagct gtagtagaag gtccaattgc cgctgcgtcg caagccaaag
     6841 acgcgggcat cgtagccgcc ggcaaggcgg taatttcctg gatcgaatac cgtgactgga
     6901 cctcctgttt gaatgtgtcc aaactcggcg agacgggccg cgagcttcgc gtagatcttg
     6961 cgataaatcg tggcctggta ttcgaacaat gcggtggtct tctcggccat ggtcgcataa
     7021 ccactgtcgt cgaagtcctc caaagcggcg ctgcccgtat ttgcgtggat cacggtcggc
     7081 gaggcctgcg ttccggagac atacacgcag cagccgttca ttttggcggt cacgaagaag
     7141 tcggccccgc acagcggcga aagtgacatc atgtggattt cgtcggtctt ccagcccaga
     7201 taatacccct ccacgtccgc cgatccatcc aatacaccgt cttgacagat cacgtcgtgt
     7261 cccgcgttct gcgccaggtc agtcgctttg agccggagtg ttcgcaggct gtcggctgga
     7321 acgtagcgca ccaaggagaa cttctctccc gagtcgatgg caattcctcc cggcgagggc
     7381 ggcgcatgcg cagttatggg catgacgcgg aatagaccgc tgtcgacgaa gcccgccgga
     7441 tcccgcttga attgaacgag tgcgcgtgta tccatatctg actcgcttat tggatggcta
     7501 ttgctgggcc cacacagcaa ggcctctgct ttacggttgt gtagtgtccg attgttaacg
     7561 gatgacccga cgtgagagca attattcagc tcgagcgcgg tggactgttg agatccgcca
     7621 gcctgacggt tctctcccgg gcaagcaccg ggcacggttg ggcgcgtccc cactcagcgc
     7681 acgcttggat aggttcctgc gctggctcgc taggggttca cctgctgccg gccgtcgagc
     7741 tgacggcggt catgcactag gcgaagaaga actttccgga gcagtctgcg cctcagtact
     7801 cgagctcacg gtactgtagc cgtatccgga ggctttcctt acgcttaatt ccagctcatt
     7861 tttcgctctg cctagctctt ctgagtgcgg cgtgctctgc cgacaacgcg ggtggcaacc
     7921 gacctggttc tggcggtggg ccccccagcg gaggagccgt cggcgttgga ggaacggtaa
     7981 gcgctggcgg cgcgactagc gccagtggtg cgacgagtac cggcggcgcg acagcgcttg
     8041 ggggtgccac ggggagcggc ggcattctga ctagtggcgg tgcgaccccg aacggtggcg
     8101 ccggaaccgg cggagtctcc tcacgtggag gcgccagcag cggcggtacc acgacggggc
     8161 ctcaaggcgg cagaaccttc ggaggcaacg gcggccaagc aatgggtgga ggagccggaa
     8221 ccggcggcgg cacggcaggc ccgattgatt gcagcacgcc catgccgacc agcggaggca
     8281 ccccgcattg tggacagaac acacaaggca aggcaggcag cctcagctgg tcactctgga
     8341 cgaacacgct gaacaacaac gcctgcatga cgacctacaa caccaccgct tttagtgcca
     8401 gctggaatgg caatggcgac ttcctggctc gcgtcggtgt tgaattcggc aacagcggta
     8461 agacctacga tcaatacgga ctcatcaaag ccgacttctc ctacaagaag acgggatctg
     8521 caggcggcta ttcctatatc ggaatctacg gctggtcgat caatccctgt gtcgagtggt
     8581 acatcgtcga tgaccgttgg ggcaccatgc ccttcgacgc ctatcaagcg acccccaagg
     8641 ggaccgccaa gattgacgac gagaactaca agctattcca gaacaagacc cctggaaccg
     8701 ggggttcgcg gtgtggcaac ggcgtgactc aatgggacca attctggagc atccgccaga
     8761 aggctcgcca gtgcggcacc atcaccatct ccgagcattt caaagcttgg gccgctgcgg
     8821 gcttaaagct cggaaacttg ctggaagcca agatcctcgt agagaccggg ggcggacagg
     8881 gtagtatcga attccccgtg gcgaacgtaa cggtcgctca gtagaagcca agcgaagcac
     8941 tcccgagcag agcctttgag gtcacggcgc caagggaggg cgcgaaacgc tctcggtgac
     9001 gaatcgagtg acaccgagag cgcgcggtaa ccaatccggt ggtcgcaccg cacctgctgg
     9061 ccacgcccag cacggcggcg cgacgggcga ctcgcaatta gcccgaaccc ttcctgaccg
     9121 tggagcccgc cctaactcgg ttgcatggct gaacgtcgga acgtcgggtg cggagctcgc
     9181 gtttacacca ctgcgcttcg ccaccttcgc cagcggccgc caccccttga gctgcatgat
     9241 ggccgtgatt tccgcttggc ataggctgcg ctttctgcgc atctgccatg tgcgctcctc
     9301 gcgagatcat accgggtcga tattggatga ttactcgtcg ctgcgtccgg cggcagttct
     9361 tgctgcggcc cgatgctcgc accaatcaat gttttctgtt ctgcctcgcc ttggcggcga
     9421 agcgctcagg agtgcaggtg gtcgccttcc tagtgatgag caaccactat catgcaatcg
     9481 tgcatgaccc gcttgggacg ttgcccgtgt ttctggagca cttgcacaag ctcacagcgc
     9541 gagccttgaa cgcgcgttgg ggtcgatggg aaaacttgtg ggcgtcggag caaacctgcg
     9601 cgacccacct ggtcaacccc gacgacgtac tcggcaaggt gctgtacact ctatgcaatc
     9661 ccgtcactga ccagctcgtc gaccgcgtga cagactggcc gggggcaaac tcctttcggg
     9721 ccatgctcag cggagaagct attgaagtgg agcggccgcg agacttcttc cgggaggatg
     9781 gaactacgcc cgaattcgcg cggctcgagc tcgtcagacc acccggattc gaagactgcg
     9841 actcgacgca gtggcgcgcg catttgagta gtttggtcac ggccatagag gaacgcacgc
     9901 gcgcggagcg acatcggacg ggaggcaaac ttctgggcag gaaagccgtc cttcgagcgt
     9961 cggcctttga tactccgcat agctcggaac cacgacgcaa cttgcgacct gccgtggcgt
    10021 gctccgatga gcaaactcgc gtgcacgtac tggcgcagct agtggcgttc cgtcgcgcgc
    10081 acgaagctgc gcgacagaga gttgccgcgg gtgaactcga cgcgctgttt ccctatggaa
    10141 catacaagat gcgtctgatg ggccatcgct gcgcgtcggt gccaccagcc tagcgaggca
    10201 gcgcattcgt ccgatcgcgc accgcgcgat ctcgggagac ctgcggcttg cgaagggaag
    10261 ccctgcggga cgctaaaggt gtatccgcag aacggcgctc tggtgaccat tctgagccct
    10321 cgctgacccg ctcaaggccc tgcccgaatc ttcgacccga cgggccatca ctggttttcg
    10381 gaactaacgt cgtacgggat cacccccgga actaacgtcg tacgggatca ccctcaacgt
    10441 cgtacgggat caccccccaa agccgtctac ggtgcagtcg tgaacgtgcg gctctccgaa
    10501 gacgcgtaga agccctgatc gtttgcccta tagagcgtag gctcttcgag gtacgcgcca
    10561 aaggaatctt gaaagccgtc tgctcccgtc gcgtcgtcga cggtgcgcag ggcattgtgc
    10621 tgctccacgc tccaggaaaa ctccgcattg cccgtgagga ccgcgtccag atcgggaatg
    10681 gtgacgcgct tacgggtcgt gaccacgtac accgcttgtt tgaacccatc ggtaatagcg
    10741 agacaatgca ccgcgttggc tcccgtccaa ctaaactccg tcttgccatt cacattcgtt
    10801 ttggcattcg tcggcgccga cagaacgggg gccgctggga tggaaagctc gattcccgtg
    10861 gagccggagc tcaccatctt gtgagcaatc gcatacgccc ctctcgtcca gtctccgtac
    10921 gaggccgcaa ccgtgattgc ggagtctgga aggcccggcg tcagatacga gaacgtgtca
    10981 ctgccgctcc agtcttcgac aaccgggatc tgcgcattgt ctttgaaatg gacgaacgcc
    11041 caattccgac gcgaggccgt gacgaggctc tcgactttgc cttgagtcga actgacgggg
    11101 aacatcgagt ccgtcaactc cacggaaatc gctgcaccgg tgttcgcgag tgaggccgga
    11161 gtggaagcgt aagattggta tgcgaccggc agatcctgat ttcccgtgaa ggaccaagtc
    11221 aggtagtgtg cgttgccgtc gatcgtgcgc ggcccgtacc aaactgccga aagataattg
    11281 acttcggcgt aattgatttc gacattcacc gagccgtgct tgctcccgaa agacatcacg
    11341 gtcttgtgtt gatcgtcgag gtaagacgtc ttggttttca gattcaggtt ggaggcgttc
    11401 tgtttctcca ctccatagta cacctggagc ttaggatctc tgcggctcag ggactcgtag
    11461 atccacgtgt attcgcgttg agtgttgttg atgattgggc ggaccgagag cctcacgtcg
    11521 taagacgcgg gcgcttcgtc caccgcgaag gccccgtctt tgtcggtctg cacggtcgtg
    11581 ctgccaatcg tgacgttcgc caacggcacc gggtgcccca aaaagtcgat tacgtgcccg
    11641 cgcacggcgc cgtcatccac ggtgtttccg cccgcgccgc cttcgccggg aatccccccg
    11701 tcgttcgttg ccccgccgct cgtgcgagtg cctccggcgc cccctcccga ggaactggcg
    11761 ccaccgttcg caggggcgcc gccctgcgtc gagccggtgt cgcccgcttg tgcgctcgtg
    11821 tcgcccgtcc cgacgctacc tccaccgcca gagcgaccgc ccgtggtggt gactccgcca
    11881 tccgagtaat aggtgcctcc ggtagaggtt ttgccaccgg ttcctacgcc tcccgttcca
    11941 acgccgctgg cgccggctcc accgctgttc cctcgcagat ccttgatgcc gatgatttct
    12001 ccacaagaaa gcgcaagccc gctcgaaagg agcgtgcacc ccaaaaccca ccgctgcttc
    12061 gttcgcatca tcagaagttt cctctcacca ccacgctgcc catacccaca ccaattcgcg
    12121 ttgcagcttt cggcggctta accgggtacg cgggatttgc cgccgcatcc ttgttcgagg
    12181 ggccgctcaa aaaccagagt accgtgcctg acgccaaacc gacgattcca actccgaagg
    12241 cgatcgtaga cgcggtcgcg gcatgccgcg cggtgtcccc gtcacgaatc gcgccttcgc
    12301 tacagttctg ataatcgccg cagccgtctt tcgcgtcgct ccaggccgag gatgctttca
    12361 gtccaaagta ggttccaacg ccgattccca caacgcccac ggcgccgagc gcgatcgcaa
    12421 cgcccttctt cgaagagctg cctgagctct cgacgcggtc gggcgttacc tggccgacgg
    12481 cgacattggt tgatttggac gtctcgcccg cctcaggcaa gcttgggatg gtgacggctt
    12541 cttgcgcgcc gtccgcccca atctccagag ctttgaacca cttgcctcgc ccgggcgcgg
    12601 tcgcttcgat ggtgtgctta ccagggtcca cggggatggc cgatccgtac tcggcactgt
    12661 ccaacaccgt gccatcgcga cggacttcga ggccgcggat ttgggtcgcc tcggtgctga
    12721 gagagatggt cagccgcgag agcttggttt cgagcgcgtc cgcccggcgg cgcgcgagct
    12781 cttcgcgttc acgcgagtcg gcctcgcggg cggcggatat cacttcgcga aactcagccc
    12841 acgcgctggc ggtgcgcccc gtcttttcgt agcactcggc caggttcagc atggttccga
    12901 cgccggggtc gagtcgctgg ctggcctcga acttgctgca cgcctcggca tagcgtgcct
    12961 cgctcatcag cttgcgcccc tctcggaaga gggcttcggc cgcgactttg tcgttactcg
    13021 ccggcggaga agccgccgtt tcggcgcgcg ccgcaggcgg gctcgccagg accaaaaggc
    13081 cgcaagtcag aaggattgga acacaccgca tcgcctcttg ggtagtccaa cttcggacgg
    13141 gcgacaaccc acggctgcta actcacgtca tgtcgaaccg cttaccattg ccgctgccgg
    13201 cgcaacgagt gttactccac gcgcagcacg aatgacgcaa agccggttcc gccgcggtca
    13261 tcgcggatga cgagccacag gcgggcctcg ctgggcacgt cgggagccgt ccactcattg
    13321 gtggattccg ggctgtctgt tttttggttc tctagtccct ccagacccac tgcctgccgc
    13381 cccgtgcggt cttgcgtgaa gctgcccgcg gtcccgaacc aggaaactcg gatggactct
    13441 cgccgctcca cgatgcgcga cgtctcgggg tcgaagtaga cgtacggctc ggcgcccgtg
    13501 caacctcgcg gctccgcgca gtcctccgcg cacgacaggt cgtcttcgta aatgctgcac
    13561 gcgccgtcgc cacacgcggc ctgcgtgggg caatcgcgcc attgcacacg caaggtgacc
    13621 cggcttcccg ctggcacgct caaagccaag ccctctgcaa gctccgattc tacgtcaccg
    13681 attcgcgcgg taacgcggtc gagctcggga ttctggttca aacgatagcg tcgcgtgaac
    13741 tccaatgact ggtcttgcgt cgcgcgggca acgccgcagg ccaagcgtgt cactcccacg
    13801 gaatactccc ccgtggtcgc gtggtgaacg cgcaccggtt gataataacc accgctcgga
    13861 tccggatcgg ccgcgcgagc cgcgggttct cccggcgcag gggcaggcgg attcggaccg
    13921 aacaagcgac acgcggtttg cggaatgacc ccggatacct gattcccacg cccgagcgaa
    13981 gagagcgcct ccgcgctatc cgccaggcat tcgagcgcga tcggcccagg taccgtaatc
    14041 ggtttgcgcg ccaagcaatg ggcgaagtcg agtgcatcgg gatcgccccc gctctgcggg
    14101 ctggccagca gcgccgtata gcgcacttcc ttgcccggcg tcacctccgc gggctcggaa
    14161 cgcaccgcca gtacgcggtc actcgtaacc cgcgaagcgc catcctcgat atccggctta
    14221 catgccgcca gcaaacagac gagcccgacg gtgcgccgac tcaccatgac aatttccctc
    14281 cgaagaccgg caagatgggc aaacccgtga tgtagctgcg ccgcgtatag gcgtaattgt
    14341 aaactacctc ttcggcgttc ttgtggttcg tcacgttttg cgcgtctaga tacagctcga
    14401 gctccgtttc accgccgaat tcgaagcgct tgg
//
LOCUS       DASTKU010000531         4564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1889222, whole genome shotgun sequence.
ACCESSION   DASTKU010000531 DASTKU010000000
VERSION     DASTKU010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889222"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFQ61_18365"
     CDS             complement(<1..401)
                     /locus_tag="VFQ61_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9956479.1"
                     /translation="MISVAMFTYSTRPRGSVVHAAALSEALRELGCEVTLHTLSKEGE
                     GFYRELSCELELWRAAPAPDDPDALIAQRIQEVASHVRRMSRTYDIYHAQDCLTASGL
                     LQSGSLRGRTQLSRSPLARSLLARTVHHVEL"
     gene            complement(398..1393)
                     /locus_tag="VFQ61_18370"
     CDS             complement(398..1393)
                     /locus_tag="VFQ61_18370"
                     /inference="COORDINATES: protein motif:HMM:TIGR04049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sll0787 family AIR synthase-like protein"
                     /protein_id="HET9956480.1"
                     /translation="MNLGGFGISELCQALRKQPELDLKRDIQLVARSLPALQTSHWFP
                     ETGPILNGDDTAALRMGDEYILFAAEGMRPEFVEADPWFAGFCSVLVNVNDVAAMGGR
                     PWAVTDVLFLGQSDNEAILAGMRYASQLLGVPIVGGHTTRVASGSALTAAVLGRARRL
                     ISSHHAVPGQRILVLYDLRGEFRNATRNFDAASRASAETLRSNLAVLPSLAEAEWVRA
                     GKDISMAGVVGSLSMLCETSRVGAVLELSRIPAPGAIEPLRWLTAFPSFGFVLAAAPD
                     CAAQVVEHCVRLGLACADVGVITERRELLLSDGTHTALYADMASEPLTGFGAESV"
     gene            complement(1390..1941)
                     /locus_tag="VFQ61_18375"
     CDS             complement(1390..1941)
                     /locus_tag="VFQ61_18375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0567/Sll0786 family nitrogen starvation
                     N-acetyltransferase"
                     /protein_id="HET9956481.1"
                     /translation="MLDRIHPWGRPLRPGLGTEVVAEVATERWQIESYWALRRSIFVA
                     EQRLFETCDRDARDPDALPIVAMSQIAGMPDRVIGVVRIYNSDGATWYGGRLGVAAEF
                     RRFGAVGTALIQVAVSTAHALGCGEFLATVQLANVRYFERHHFSVRGSIDVAGQPHRL
                     MEASLDHYPPRAIGSRFCQTRAA"
     gene            complement(1934..3097)
                     /locus_tag="VFQ61_18380"
     CDS             complement(1934..3097)
                     /locus_tag="VFQ61_18380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0568 family radical SAM protein"
                     /protein_id="HET9956482.1"
                     /translation="MHRSLPIANPSPPALRASVPTPASWPELLAELQAVGLRWLDPQG
                     PGLNRKGGAGPSDHKAITLASHTVMVPIHTRTAERSPYSALVDVDGESALLIRRGKPI
                     AELTFPREPRFYSLSTADGTPYWKIATLHGRDVLASTVLQTCTRYGKRESSCQFCAIG
                     QSLAAHATIAEKTPAQLAEVAEAAVRLDSVTHAVLTTGTPHTPDRGAEHLCNVVRGMR
                     ERVALPTQVQCEPPADLRWLDRLKQAGADALGMHLESVSEPVRQRIMPGKASVPIEFY
                     LRAFEHAVPIFGRGQVSTYILAGLGDTREEILNMSERLLTLGVYPFVVPFVPISGTPL
                     EHATPPDPAFMQALLADVAALISRAAMKSSSIKAGCGRCGACSSLAVREAHRA"
     gene            complement(3018..4073)
                     /locus_tag="VFQ61_18385"
     CDS             complement(3018..4073)
                     /locus_tag="VFQ61_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000257 - nitrilase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nit6803 family nitrilase"
                     /protein_id="HET9956483.1"
                     /translation="MGNRTVRAAAVQLSPVLFNREGTTQKVCEAIAMAGRQGAELVVF
                     PETIIPYYPYFSFIKPPAVVGNEHLQLIQESVAVPGPTTDSLSRAAREAGAVVVVGVN
                     ERDHGTLYNTQLVFDADGQLIQARRKITPTYHERMIWGQGDGSGLCAVDTRVGRIGAL
                     ACWEHYNPLARYALMADHEEIHAAMFPGSLVGNIFREQIEVTIRHHALESGCFVVNAT
                     GYLNPEQVAQLAHGTQLDRALQGGCFTTIVSPEGSLLAEPILEGEGIAYANLDMQLVA
                     KRKRMMDSVGHYSRPELLSLLVNRDPQPHVRPTSGLSNAPNRQLATRLPDASIASDRE
                     PVPTSSASERPDARVLA"
     gene            complement(4129..>4564)
                     /locus_tag="VFQ61_18390"
     CDS             complement(4129..>4564)
                     /locus_tag="VFQ61_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MSMEG_0572/Sll0783 family nitrogen starvation
                     response protein"
                     /protein_id="HET9956484.1"
                     /translation="DYEEKVFEDVRAEPGEKALVTFHTVAFEGSIGLVNLLQAIRLQR
                     KGFETSVLLYGPGVTLGVQRGFPKLGDEAFPGHQNFNNQIKKFMSEGGKVYACRFALQ
                     ALYGHGEKALIEGIRPINPLDVLDIILLHRKQNAFILDSWTL"
BASE COUNT          882 a         1430 c         1441 g          811 t
ORIGIN      
        1 tatagctcca catggtgcac ggttcgggcc agcagcgagc gagcgagcgg actgcgggaa
       61 agctgagtgc gcccacgcaa gctcccactc tggaggagcc cgctcgccgt caggcagtct
      121 tgggcgtggt agatgtcgta ggttcgcgac atgcgacgga cgtgactcgc gacttcctga
      181 atacgctgcg caatgagcgc gtcaggatca tccggcgctg gagcggcacg ccacaattcg
      241 agctcgcagg atagttcgcg atagaagcct tctccctctt tggagagggt gtgtagggtg
      301 acttcgcaac ccagctctcg tagcgcctca ctcagcgccg cagcgtggac cacgcttccc
      361 cgcgggcgag tggagtaggt gaacatggcg acggaaatca taccgattct gctccaaagc
      421 ccgtgagggg ttcgctcgcc atgtccgcat agagagccgt atgggtgccg tcgctcagga
      481 ggagctctcg gcgctcggtg atcacgccga cgtccgcgca ggcgaggccc aatcgcacgc
      541 agtgctccac cacttgcgct gcacagtcgg gagccgcggc tagcacgaaa ccaaagcttg
      601 gaaacgccgt gagccagcga agcggctcga tcgctcctgg cgcaggaatg cgcgagagct
      661 cgagcacggc ccctactcgg gacgtctcgc acagcatcga caacgacccc accacgcctg
      721 ccatgctgat gtctttgccc gcgcgcaccc attcggcctc ggccaggctc ggcaacacgg
      781 caagattgct ccggagcgtc tccgcggagg cccggctcgc cgcgtcgaag ttacgcgtcg
      841 cgttcctgaa ctcaccgcgc aagtcgtaga gaaccaagat gcgctggccg gggaccgcgt
      901 gatggctcga gatcaggcgc cgggctcgcc ccaggactgc ggcggtaagt gcgctgccgc
      961 tggcgacgcg cgtggtgtgc ccgccgacga tgggcacccc caagagctgc gaggcgtaac
     1021 gcatgcccgc aaggatcgct tcgttgtcgc tttggccgag gaatagtacg tcagtcaccg
     1081 cccagggccg accgcccatc gccgccacgt cattcacgtt gacgagcacc gagcaaaacc
     1141 ccgcgaacca aggatccgcc tccacgaatt cgggacgcat accctcggcc gcgaacagga
     1201 tgtattcatc tcccattcga agagcggccg tatcgtcacc gttgaggatg ggcccggttt
     1261 cgggaaacca atggctcgtt tggagcgcag gtaaactgcg agccacgagt tgaatgtccc
     1321 tcttcagatc gagctcgggc tgcttgcgca gcgcctggca tagctccgaa atcccgaagc
     1381 ccccaaggtt cacgcggccc tcgtttggca aaagcgggag ccaatcgcac gcggcggata
     1441 gtggtcgagt gacgcctcca tcagccggtg aggctgaccc gcaacgtcga tcgaaccacg
     1501 aacactgaag tgatgcctct cgaagtagcg cacgttggcg agctgcacgg tggcgaggaa
     1561 ctcaccacag ccaagggcat gagccgtgct caccgccact tgaatgagcg ccgtaccaac
     1621 tgccccgaag cgacgaaact ccgcggcgac tccgaggcga cccccatacc aagtcgcgcc
     1681 atccgagttg tagatgcgga ccacgccaat cacacggtcc ggcatgccgg caatttggct
     1741 catggccacg atgggcagag catccgggtc acgagcatcg cggtcgcaag tttcgaacaa
     1801 cctctgctcg gcgacgaaga tcgatcggcg cagcgcccaa tacgactcga tttgccagcg
     1861 ttcggtcgca acttcggcca cgacctccgt acccagcccc gggcgaagag ggcgccccca
     1921 cggatggatg cgatcaagca cgatgggcct cccgcaccgc gagtgacgaa caggctccgc
     1981 agcgcccgca gcccgccttg atgctgctgc tcttcatggc cgcccgcgag atcaaggcgg
     2041 ccacatcggc cagcagagct tgcatgaacg cgggatcggg cggtgtcgcg tgttcgagcg
     2101 gagtccccga aatcggaacg aatggcacca cgaaggggta gaccccgagc gtgagcagac
     2161 gctcgctcat gttcaggatc tcttctcgtg tgtcccccag ccccgccaga atataggtgg
     2221 acacttggcc gcggccaaag atgggcaccg cgtgctcgaa ggcccgcaga tagaactcga
     2281 ttggaacgct ggctttgcca ggcataatgc gctgccgcac cggttcgctg actgattcca
     2341 agtgcattcc gagcgcatcg gcgcccgctt gcttgagtcg atcgagccag cgcaaatctg
     2401 cgggaggttc gcactgaact tgggtgggca gggccacccg ctcgcgcatt ccgcgcacta
     2461 cgttgcagag gtgctccgca ccccgatcgg gcgtgtgcgg ggtgccggtc gtcagtacgg
     2521 cgtgggtgac ggagtccagc cgcaccgcgg cttcagccac ttccgcgagt tgcgcggggg
     2581 tcttctcggc gattgtggcg tgggccgcga gtgattgacc aattgcgcag aactggcacg
     2641 acgactcgcg cttgccgtag cgcgtgcagg tttgcagcac cgtgctcgcc aaaacgtcgc
     2701 ggccgtgcag ggtcgcgatc ttccaatatg gcgtaccgtc cgcggtgctc agcgagtaga
     2761 agcgtggttc gcggggaaac gtgagctctg cgatcggttt tccgcggcgt atcaagagcg
     2821 ccgactcgcc gtcgacgtcc acgagagcgc tatagggaga gcgctccgcc gtgcgggtgt
     2881 ggattggaac catgaccgtg tgcgaagcga gcgtgatcgc cttgtgatcc gaggggcccg
     2941 cgccaccttt gcggtttagc ccggggccct gcggatctag ccaacgcagt ccgaccgcct
     3001 gcagctctgc caggagctca ggccaagacg cgggcgtcgg gacgctcgct cgcagagctg
     3061 gtggggacgg gttcgcgatc ggaagcgatc gatgcatcag ggagcctcgt tgcgagttgg
     3121 cggtttggag cgttggagag acccgacgtg gggcgcacgt ggggttgagg gtctcgattc
     3181 acgagcaagg aaagcagctc gggacgactg tagtggccga cgctgtccat catgcgcttg
     3241 cgcttggcca ccaactgcat atccagattc gcgtaagcta tcccctcccc ctcgaggatg
     3301 ggctccgcca gcaagctgcc ctccggagag acgatagtcg tgaagcagcc cccttgtagg
     3361 gctcgatcca gctgcgtacc atgcgcgagc tgggcaacct gctcgggatt caagtaaccg
     3421 gtagcgttca cgacgaagca gcccgactcg agcgcgtgat ggcgtatcgt gacttcgatc
     3481 tgttcccgaa agatgttgcc gacgagtgag cccggaaaca tcgcggcgtg gatttcctca
     3541 tggtccgcca tcaacgcata gcgggccagc ggattgtagt gctcccagca ggcgagtgcg
     3601 ccgatgcgcc cgacgcgggt atcgacggcg cagagcccgg atccatcccc ctgtccccaa
     3661 atcatgcgct cgtgataggt cggggtgatt ttgcggcgag cttgaatgag ctggccgtcc
     3721 gcgtcgaata ctagctgagt gttgtatagg gtgccgtggt ctcgctcatt cacgccgacg
     3781 acgacgaccg cccccgcctc gcgtgctgcg cgtgatagcg agtccgtggt tggccccgga
     3841 accgccacgg actcttgaat gagctgcagg tgctcattcc ccaccacggc tggcggcttg
     3901 atgaacgaga agtagggata atacggaata atcgtttccg gaaacacgac caactccgcg
     3961 ccttgccggc ccgccatcgc gattgcctca cacactttct gcgtggtgcc ctcgcgattg
     4021 aacaagacag ggctcagctg tacggctgca gcacgaacgg tacgattacc cacggtgttc
     4081 accaggcttt tgcgagagcc gaaatcaatg gcaaggcacg tgagaagctc agagtgtcca
     4141 actatccagg atgaaagcgt tctgtttgcg atgcagcagg atgatgtcca gcacgtccaa
     4201 ggggtttatg ggtcgaatgc cctcgatcaa tgccttctcg ccgtggccgt agagcgcctg
     4261 cagagcgaag cggcacgcgt agacctttcc gccctctgac atgaacttct tgatttggtt
     4321 gttgaagttt tggtgaccag gaaaggcctc atcaccgagc tttggaaaac cacgttgaac
     4381 gccgagcgtc acgcccggtc catagagcag aacggatgtc tcgaagcctt tcctctgaag
     4441 ccgaatggcc tgcagcagat tcacgagtcc aatggagcct tcgaaagcca ccgtgtgaaa
     4501 cgtgaccaga gccttttcac cgggctcggc gcggacgtct tcaaatacct tctcttcgta
     4561 atcg
//
LOCUS       DASTKU010000532        37291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_326231, whole genome shotgun sequence.
ACCESSION   DASTKU010000532 DASTKU010000000
VERSION     DASTKU010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 37291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37291
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326231"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VFQ61_18395"
     CDS             <1..1029
                     /locus_tag="VFQ61_18395"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9956485.1"
                     /translation="IEFYRRALGARILARYEHGLERHVSHSDLALGDARFSVTEEAKA
                     WNSIAPSSLGGSPVVLQWGVADADDALQRFVRAGGTVIHPLQPLLGERMARLADPFGH
                     VWLLRQKVQDLTVAESQRQRDELFERFTRTQPPEEPARHPAVGLETEVRLSHDPVSCQ
                     AGISPRDGLYERSPRVHLVVGPVGAGKSTLALQLARQHRALRLTLDAWMTTLFSRDRP
                     ERALIPWYIERAERCTQQIWSVAQAALEIGTDVVLEIGLIQREPRLRFWDRLTESGAD
                     VVMYVVEADREVRRRRVEARNRERGATFSMLVPPDVFELASDLWEAPDASECADRQVV
                     FLRTDPAP"
     gene            complement(1058..1597)
                     /locus_tag="VFQ61_18400"
     CDS             complement(1058..1597)
                     /locus_tag="VFQ61_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956486.1"
                     /translation="MENPSSVALDLASRTDVPILANLLELYIHDICDLFPKVELGEDG
                     RFGYPKLASYFGAAESGTNGGRFAYLIRHGGRLAGFALATRGSPVSPDPAVFDVAEFF
                     VVRKYRRLGVGYGAAALLWSAWPGTWTVRVSEGNMRGLGFWDSAIRRFAGEDVTVSTH
                     PGAPHAWRVFSFATKASPG"
     gene            complement(1729..3459)
                     /locus_tag="VFQ61_18405"
     CDS             complement(1729..3459)
                     /locus_tag="VFQ61_18405"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9956487.1"
                     /translation="MRGIGAMGGIGRTGYSAARRLAWLGLCGGSLWGCSGESDSQKVL
                     GPDQDTAQAAFFSATRRVEVEIELAPADWDVLREQGRALTSFLELNEPYEYTYFDATV
                     RVDGERYEHVQLRKKGFLGSLSALRPSLKLDLDRVVEGQNHVGLHSLTLNNDRQDPSH
                     THQCLAYGMFSDAGLPASRCNLAHVVVNGEDLGTYSNVESISKQFLKRHFKSAEGNLY
                     EGQAADFDAVRVEQLQLKTNEMSNDRSDARALASALEAPDEQAVSTIGKQLDLGKFRD
                     FWAMETLLGHWDSYSGNANNYFVYRDPASQRFVFLPWGVDAAFEGLNPFDPLNQNIAV
                     YAAGRVANRLYHLPQEREKYRARLGELNESVWDVDRLLKQIDRIAALAPDADPKQLER
                     QREHVRNHPGLLRAALQQPAPEWQENTTAFTSPCLGMLTDMSGSIDTSFGSLQEPLPG
                     ANQLSLNLDGKLVELSWVARAGLDESGDGVPRIRYMGMLEDQRLVVVEISMPPGRFSP
                     GHHLFHGIETSGSVLVLGQNTFTLVGLIGDGAVDLEEAGTEMDAPVRGSFAGHVLQFS
                     CAELEGTAGE"
     gene            4976..6622
                     /locus_tag="VFQ61_18410"
     CDS             4976..6622
                     /locus_tag="VFQ61_18410"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9956488.1"
                     /translation="MKQSSTRPAAVARSSEPAPYSLLVCTPERDAWSFPIVPGRGYTI
                     GRNAASDIVLDYPWVSHTHAVISGADPPQILDLGSRSGTLVGTRRLEAGTLCALPVGS
                     LVEIGSISLLVQRSEEVTRDVCWPRHTAGGPVPDSNVRVQHPLGAAAFVSRNREMRRL
                     YAMLRQVAQSNVSVLILGETGVGKELFAQAIHQHSPRSANPIVILNCAAVPESLVESE
                     LFGYQRGAFSGASVPKPGLFESAHDSTFFLDEVGELPVTVQPKLLRVLETGDVQRLGA
                     LRSKRVNVRIVAATNVDVQAAIARGEFRADLFYRLNGITLSVPPLRERREDILELAEY
                     FVERSGVPCAIDREREPLFTPEARSALFAYAWPGNVRELKAVVERAALLAGGGVIDAE
                     HLLFDPPVIPSWPSHAKRLSMLPSANETLQSGPISSGPRSAGSSGGPPSNGPRSSGSQ
                     VSSLPPSNIPSSRWPSGEPGRLSSAAPPNDDLTFSSQELPTSVVRAELERRERRRIQE
                     ALDRTGGNQKNAAILLGMSRRTLLKRLDRYEMARPRKRRE"
     gene            7105..7860
                     /locus_tag="VFQ61_18415"
     CDS             7105..7860
                     /locus_tag="VFQ61_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956489.1"
                     /translation="MISRRRFGSHCGVAGSFVLAMACQSVPDASDSSETVTLTLPNGA
                     CNQAQPEDVLIAQLVKDSWVAQYPLTRLFVDSSSNVTGPSLPAILQFDLDVINGVPAA
                     KTAVSAAVATISGLPDYQPDGFGPETPACSAVPAWTPNGITQVATTEVEVFTTAVNQD
                     SWRKTHKAFGKECPLLRRTGNQDLLDPPGDGSTNMPPGGTVSATGVTANAYGLCPTGT
                     APGTFCKLSYATGVNYMGRKCLYYYGSLRCLLY"
     gene            8162..9091
                     /locus_tag="VFQ61_18420"
     CDS             8162..9091
                     /locus_tag="VFQ61_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956490.1"
                     /translation="MNYNRYSRCDFTGGLLGWASVLASMTAVLACDDDSGQPALTQCP
                     TGGGSGGSSSTSDTSGGVSEGAASGRTTASAEGGTTSAGTTVSFGGTSAKDPQTGGSK
                     AMGGVTQVATEPEGGAGGTEPTTDVPTEGGAGGGTATTEPNPPDTNSWTSICEDNMGM
                     FLPYDFSRGIAGEYAFELSVSCDLGGYLLPLVVLDPVNLTGVDAWVTQLTDWLRGRVL
                     VCNDDFSKLPPDDFSILPSSQRAGMSRGDFNSSVQLFLSVMARHDGLPDGINPEFKTV
                     LQERLASYAESTVSVQSDALTKQLSPPDCIPPL"
     gene            9146..11065
                     /locus_tag="VFQ61_18425"
     CDS             9146..11065
                     /locus_tag="VFQ61_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956491.1"
                     /translation="MIAQSARAEIPFLDAQRASCISRTSSPNAPRSVRNLLERSMSYF
                     HGLSETMQCLARSATRAIEASVCAANARKRRQRHGTWVHGVTAQVLLRVTAVVIWAGV
                     GGCTYLLDLEPNQCKVDADCLSRPGFEGTSCVAGSCKTSLSAAGSTQGPPKDGCTSNT
                     KCVADHNNQPYACIEPGQDCVPLTTEECPVVFGNYNDDNALYFGALLNVPSNAPLSQQ
                     STLNVQLAVDEFNGSVGGLPGGPLGKLRPLVGVVCRNEPELVPAESSHLFDRIGVAAT
                     IAHLPSGELKSFLVDRALPKQAFVMNPGFADNSLTSLSSQGLFWHVIGDIRDVAPAYP
                     PLVARLEKMIKAGGSDEPLRVAMLVSRRYAEQSIADTLAAQLEFNGNSAAENGSAFYT
                     AAVPVLADEPSADYTSVVDALLKFRPNVVIAITREELVYKILPDVEERWPRDQLVRPY
                     YILPNALSGNQNLLDYVAYDSRAVSSENKRQRMVGVAPASAEDLQLYNQFLLRFRAAY
                     PTFPNPGGFENFYDAVYLVANAMYAAGSVPRLTGPDVARGMQRVIAGPIVVNAGPTFI
                     ADGFSALAAGGNIRLNGTMGLADFDPGVGARRGNGSIYCIQRRGSQLGFVYDVLRYDK
                     SSKTLKGEFPCFPGL"
     gene            11105..12922
                     /locus_tag="VFQ61_18430"
     CDS             11105..12922
                     /locus_tag="VFQ61_18430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9956492.1"
                     /translation="MGQRTMYTAELFPDMTDEPLQAGDLLGKYQLVCPVARGGMGQVW
                     AARQTGLLGLPGLVAIKTALPSSDRTLESMQTLLFDEARVAASIDHPNICKILELGFE
                     RGVLFIAMEWIFGVPLSNLLSLVPNRKLDCSTAAYILAQACRGLHAAHELTDEEGHPL
                     EVVHRDATPHNMLITLTGDLKIVDFGIVKARNQLHQVTETGELKGKISYLAPEQLRGK
                     GVDRRSDIFTLGCVLYLATTGRAPFNGLEAGTTVMRIMNGEYAPPSSLVEDYPEQLEQ
                     IVCRALALDPNARFQTAQEMQYALEAFLNGQDGRAAVARTVEEYCGAIVEQRRADIRA
                     AQKLLDSQAGHMASGTYRTGTRSPESEGSWREEALRLLSTAAKTPATQSELVPTQAAR
                     SPRHRWLIGGSVALVGCVGALIVASQLQRSDARRASEGSALRVPSASNVAAASVTPAA
                     RASTAPARTTREPINLTLQSRPEGAKVRVDGGPAQATPRTLQVPPDDKEHVITLQAPG
                     YEDIRQTVRFNRDQVFHFDLLPKRVTAGGRGLPRSTDAGLTKAQIAAAARAAPERARQ
                     SNETSAPQPSTQLEPTLQPLLRQAPRHQIDQTDPFARQR"
     gene            12922..13971
                     /locus_tag="VFQ61_18435"
     CDS             12922..13971
                     /locus_tag="VFQ61_18435"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9956493.1"
                     /translation="MSPTARAVACGLIATQCLLARSAFADSPPSEGPSGAPAGEPVRV
                     ETDRPAADRAVSNPEPDKVELARVHFLRAVQFFNDGDFKLSLVEFRRAYEISGNYRVL
                     YNIGQVNHQLSNYTKALTSLERYLAEGGDDVPEDRRQEVSQSIVELNKKVARVRLITN
                     VDVAELWLDDDSQGEIHPGQVVVIDAGDHRLELRRQGYRSALTQVSLTAGENSDLTLN
                     LARAPRQMPMPVRYEPPVSRGMPAGVWLGWFVTGATAVGAVVTGVVAQKHAKDLHDLR
                     DSPTSTSEERENARDRARSFAIASDALTGTALAVGAVSLYFTLSVSAANRRGGVDPLT
                     QLALGPGSFTLRRQF"
     gene            complement(14007..15605)
                     /locus_tag="VFQ61_18440"
     CDS             complement(14007..15605)
                     /locus_tag="VFQ61_18440"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET9956494.1"
                     /translation="MQTRVQSAGLAQTLIAYNFIGGRESPAGLNALARRNPAFRDDVV
                     TLAPQSSREEVITACQAARAAQVGWARTPAPQRAQVLGRLGKLLTESKESLARFIARE
                     VGKPLREARGSVQEAIDTAEFFQSEGRRLYGQTVPSELANKELFTYRRPLGVVGVITA
                     GNFPIAVPAWKLIPAILCGNCVVWKPSDDAPGIATLFLELWRRAGLPQGVLNVVHGAG
                     AGHAGEFLVQAVEQGLLDKISFTGSTEVGRKIGEVCGRALQVPSLELGGKNPLVILAD
                     ADLDLAIDGAVWAAFGTAGQRCTSAGNIIVDKRVMPVVRSELVRRARALSIGDPSNER
                     MDLGPLINERFLEQWLLQRGTGLKDGAELLLDGKQIQPGEEPPNFSGDASKGLYASPR
                     IFDRVKMNMRIAQEECFGPTVNLVEVDGVDEALAAANGTPYGLSSAIYTRDARCMLRF
                     KEEISAGMTSINNSTTGAEAHLPFGGNGWSGNGTRESGIWVLDAYTRWQAVNVDLSGR
                     LQKAQIDLPDSAQLEPLAIEGLLR"
     gene            16030..16998
                     /locus_tag="VFQ61_18445"
     CDS             16030..16998
                     /locus_tag="VFQ61_18445"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9956495.1"
                     /translation="MARSQFLPAAIAVACYAAEAPKPAIHSSASLASLIDVPSLGAAR
                     APDAAAGTPRLKSGALVAAAPGSQQLASATLGASSPVRSLCAPEMAEVQRPEGTYCID
                     RWEGSLVDRAGAPWPNNQSLDKPDAPAGDLIAVSAPGRLPQGYISGAQAARACRNAGK
                     RLCELDEWAHACRGSHGYQHPYGNERRVGACNEPPRRSAGHPVMMLFDRFAKPGTDRA
                     TMWNSEWMNDPRLHEMPFGLEPTGSRTECKSEAGVYDMVGNLHEWIADPEGTFVGGFF
                     LDTTLNGEGCAYRTRAHDFDYHDYSTGFRCCADVSEGASAAWAQRR"
     gene            complement(17049..17606)
                     /locus_tag="VFQ61_18450"
     CDS             complement(17049..17606)
                     /locus_tag="VFQ61_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956496.1"
                     /translation="MAVGGGLRAQLGLASLAIAIGAGGSSACGDEGESRFLPTSNRPH
                     HRPASVPEIGTSGGATSGPSAGVGGETTENGGTPAQGGAPAQSGGEPGTGALGGSAGA
                     NNAGASSVFGGAWSCGVAAGLCSCVHVDLELEAPGLLGSCAASDCCFEQQDGTCSCRM
                     PDALLDCARLASALGATRARSRCPE"
     gene            complement(17597..18679)
                     /locus_tag="VFQ61_18455"
     CDS             complement(17597..18679)
                     /locus_tag="VFQ61_18455"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HET9956497.1"
                     /translation="MRPKLTRSVWLLCLIGGLGATSAGCTNSNVSSTFQTLESEPDTD
                     ADGLADSVDPCPTGLENEGTPLVVDEPSPQYLEFCTPEPQSVQLVPPQVHGACEPPLL
                     RARLVQVNGHTLAEPRDATEEQLLLLPMGSHRLTWSVRDSGGKLYATREVGVELRHTP
                     SARCATADQFVMSIQPDAPAAIAWNATLWLGSDEADLLLGSANSDFIWGSTNDDYLAS
                     RGGNDVILGGPGNDYLTAAGGGNVMLFGGAGADVIHAQSAARAVLYGGTGADVLIGSA
                     GDDVAFAGPETRLVALGNGDDALRIEHPCELESEAHWDGGPGDDTLYSPVDQATLERA
                     GVRVDGFEHIVVDTSRGYLSECFTWL"
     gene            complement(18992..20068)
                     /locus_tag="VFQ61_18460"
     CDS             complement(18992..20068)
                     /locus_tag="VFQ61_18460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET9956498.1"
                     /translation="MPHRGHPARDVSVSCAKLTGTNLAVARLVPSSQTLRRPFKLPAW
                     VGTARRLIEETLDEYSKDRGDVIAAALAFNTLMSIAPFIIIAVAIAGAVLGRGAAERE
                     VSEMLTQTMGTKAATTIEGWVREASDSQGVASVAGFALVIYTASRMTAQLRDALNQVW
                     NVDVYQAAGFKDSVRDYVKRRAVALLLVIAAGPLLLLVFLSRTLLTGLHEVLFASSPA
                     AGVLVQLGQFVFSLLLVAAISAVVFKLLPDTRVGWHAVTRGALLTSVLFNIGNSLVGL
                     YLGRASVTQTYGAAGSAIVVLLWLYFSAQMFIYGAEFTQVYAKHYGRGLTPEEQRDVA
                     LAEREGHRKEAESAAHTAAPHSIN"
     gene            complement(20114..21913)
                     /locus_tag="VFQ61_18465"
     CDS             complement(20114..21913)
                     /locus_tag="VFQ61_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET9956499.1"
                     /translation="MRIEDYAIIGDTQTLALVSKAGSIDWLCFPRFDSGACFAALLGT
                     PDNGRWRIAPHVNRERTAVAPKVARRYRQDTLILETTFETEDGAVRLVDFMPPRGQSP
                     DVVRIVEGLSGCVPMQSELIIRFDYGSIVPWVRNLDGRLHAIAGPDALQLDTPIKHCG
                     RGLTTVADFEIRAGDRVPFVLTWHASHQALPESPDPFHALRETEEWWREWVSHCTDSG
                     EYRDAIVRSLITLKALTYGPTGAIVAAGTSSLPEAIGGVRNWDYRYCWLRDSTFTLYA
                     LMSGGYEQEACAFRDWLLRAAAGDPSKLQIMYGISGERRLEERTLDWLVGYEESRPVR
                     IGNAAAAQLQLDVYGELSDTLHQSRRAGMTPRDPAWPLQRALTEWLESNWSSADEGLW
                     EVRGPRRQFTHSKVMAWVAFDRAVKAIEQFGLPGPLERWRATRERIHEEVCRQAWNSE
                     LRSFTQYYGGRGLDAALLLIPAVGFLPATDARVIGTVQRIERELIENGFVLRYRSDGV
                     DGLPGHEGAFLACSFWLVDALTLLGRRNDAQQLYERLVAVTNDVGLLSEEYDTLNGRL
                     VGNFPQAFSHVALVNSARNLTSTRQPSEERGRT"
     gene            complement(22127..23164)
                     /locus_tag="VFQ61_18470"
     CDS             complement(22127..23164)
                     /locus_tag="VFQ61_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956500.1"
                     /translation="MRSRVWIGGFGFAAICGIFVFAACGSDSSSNNNAAGGSTAKGGA
                     TGVTGGSTGTTGGATGTTGGATGTTGGTTATGGGGGATGGATAQGFACAGKVADCSTI
                     SEFPTGTAQTWGSADFGGGYSTYGDLKREGTDTGALHLTGTIKTYSGITVWFNSCSDV
                     SAYTGVSFKITGTAGAKVQFQLQTNSTYPYEANPADMKGSCKATDATKPWDGPCVAAK
                     KTEDIDVATATSPVTMTWADISGGGPTAWSAATSTKEMLGLQWQFAPVATMADGYPVD
                     ITIDDITLTGGTQAACKVVGTAGGAGGGGAGGTAGGGAAGGGAAGGGAGGTAGAGTAG
                     GGAGGTAGTTT"
     gene            complement(23546..24922)
                     /locus_tag="VFQ61_18475"
     CDS             complement(23546..24922)
                     /locus_tag="VFQ61_18475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956501.1"
                     /translation="MTRRSFGAHALRLAKGLACALGLAGCGESTQETTAELGHSPASR
                     LTECPELDTSICDIGTPECQRRMWDIARCLRGGAEEAMPPISVMTPTAYAAELRAELP
                     AQPSTEVKSYETALAMLGLMPPGALESDAYVAEQLSWLGGVYRPEKGDIVVLDRTSQP
                     QPRTIIGEGLYAQSLEWHSLSPTLVHEFVHALQDREIGLNDFSEAHSSSSDASWAAQA
                     VIEGEARWLDVRYSAATLGLDPEKVDWQSYFENSIERSSREQLVAGKTPLQSLRRTLV
                     YFWGGRFVSEAWQKRKHAGVVELQLDPPEHALTVMGGHTPELPAASFAGLAPGAEWEL
                     VGDDELGSLGVLAAMSSAGGMSAVPDFDAALEIALAWRGDELKVFTSRSESGGSPTDA
                     GTPSGSHVLVAWQIEFAQEASANAFLERQRQRGEFTRSSSLLTRTGTRVLVLAATRAV
                     DWGFADTG"
     gene            complement(24919..25440)
                     /locus_tag="VFQ61_18480"
     CDS             complement(24919..25440)
                     /locus_tag="VFQ61_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956502.1"
                     /translation="MRARAGVLALAAWLGGFAVACSGEDGPPSSADIACGGLCEAAFR
                     CGLNPDRANCRAACTQDSSLSAIPRHAAQAYGQCVQRLSCAALDDQTLWNEEFKPCFL
                     EELYRAPVTQRAKDFCALQVELYLECGWLYPRDVCEMEMSIASDAEFSALDACRAREE
                     CSEYKACNEAVFE"
     gene            25950..27914
                     /locus_tag="VFQ61_18485"
     CDS             25950..27914
                     /locus_tag="VFQ61_18485"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HET9956503.1"
                     /translation="MPQTNTPSEFPRLIIEQVTPILDAGRYPIKRRAGSHIDVGAAIF
                     KDGHDLIAADLVYRGPGDSEARRVPLNYRYEADRWYGMFHADRAGRWQYWIEAWPDAF
                     GTFSSELKKRVGAGQDVRSELLEGAALLLKHAEGLSGATKLRVLDAAHVLKNPERSME
                     ERLALAFSEELVSSVRHPLPGELCESPTLELISDPPKASFSAWYELFPRSESGVPGRH
                     GTFADTERRLPELAKMGFEVLYLPPIHPIGHTHRKGRNNARVAEPGDVGSPWAIGSEQ
                     GGHDAIHPDLGSIEDFRRLVRAAGELGIDIALDFALQCSPDHPWCKEHPEWFFIRPDG
                     SIRYAENPPKKYEDIYPINFWCENREALWNACRDVLFYWIENGVRVFRVDNPHTKPLA
                     FWEWVIREVHLRFPDVIFLSESFTRPNRLKALAKLGFNQSYTYFTWKNSSWELKEYMN
                     ELIQPEMLSYFRPNFFANTPDILHEYLQVGGRPAFRIRLVLAATLSPTYGIYSGYELC
                     ENVPVRHGSEEYLDSEKYELKTRNYDAPNHIKEDILKLNRIRRENPALHELGNLRFCV
                     SDYEGILAYRKSAPNNELLICVNLDPFSVHETMVHVPIAELGIPEGEPYEVFDLLTHE
                     RYVWRGSRNYVRLDPAERVAHVFRISPRPL"
     gene            27935..30061
                     /gene="glgX"
                     /locus_tag="VFQ61_18490"
     CDS             27935..30061
                     /gene="glgX"
                     /locus_tag="VFQ61_18490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET9956504.1"
                     /translation="MRVRPGKAHPFGATWDGEGVNFAIFSADATAVELCLFDSVDAKK
                     ESFQIKLKERTNRVWHVYLPDLRPGQLYGYRVHGPYAPLQGHRFNPNKLLLDPYAKAI
                     AGGSRLADEHFGYRIGDPAMDLSFSETDSAPVMPKSVVVEGAFSWGDDRPPLVPWSRT
                     LIYECHVKAMTMRHPEIPGHLRGTYLGLCSEPIIDHLKSLSVTAVQLLPVQHAISERR
                     LTDMNMINYWGYNTIGFFAPDPRFATGALGQQVTEFKTMVKVLHRAGIEVILDVVYNH
                     TAEGNELGPTLCFRGVDQCAYYKLSPNNPRHHIDYTGCGNSFNLSNPRALQLVLDSLR
                     YWVQEMHVDGFRFDLAVTLARDPYEFDMFSRFFATVQQDPVLSHVKLIAEPWDIGPGG
                     YRVGGFPPGWAEWNGRYRDCVRRFWRGDEGQVPELASRLSGSSDLFHGGDRGPFASVN
                     FVTCHDGFTLHDLVSYEHKHNEANAEENRDGTNDNQSRNWGVEGETRAQQVQRLRDRM
                     KRNMLALLAFSQGVPMISHGDEIGRTQRGNNNAYCQDNDLTWLQWDLDARQRELLAFT
                     REVFRITNSNPVFRRRRFFDGDPVSTSGVKDVSWVRPEGGEMTNEDWGNPKNHSLGML
                     IHGEASDDVDERGRPNRGQTLLLLLNASVRARSFVLPVLPEAGHWQEVVNTAQSTKRI
                     PKGPAISVAPHSLVLLCYVPGSADKV"
     gene            30121..32331
                     /gene="glgB"
                     /locus_tag="VFQ61_18495"
     CDS             30121..32331
                     /gene="glgB"
                     /locus_tag="VFQ61_18495"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET9956505.1"
                     /translation="MDSLNVDPVLAGDPEKLEALRRDRLHDPHSVLGAHPTRVGYSEG
                     IVVRSYHPEAEGCALLLGSEVRPMTPLGGGIFATFLEGARLPISYRLRFSFEGGAVWE
                     RDDPYRFLPTLGELDQHLFSEGTHRRLWEVLGARVRVQEGTSGTAFAVWAPGARRVSL
                     VGDFNRWDGRLFPMRSLGSAGVWEIFVPGLGAGALYKFEIKTQDGALRIKTDPMAREM
                     EHPPATASRVNEARHIWGDDAWMQARAGRDIRREPVTIYELHLGSWARVPEEGNRFLS
                     YREIAHRLVAHVQKLGFTHVELMPVAEHAFYPSWGYQVTGYYAPTSRYGTPDDFRYFV
                     DYCHQHGIGVIIDWVPAHFPKDDFALRRFDGTGLYEHEDMRMGEHPDWGTLIFNYGRR
                     EVRNFLVANALYWLREFHVDGLRVDAVASMLYLDYSRKEGEWLPNPFGGRENLDAIDF
                     LRAFNSAITEEVPGAFTIAEESTAWGGITRPPAEGGLGFTFKWNMGWMHDTLAFFTKE
                     PVHRKYHVDQLTFAMLYEHTESFINSISHDEVVHGKGALVEKMPGDFWQKLANLRLLL
                     TYQYTRPGKQLVFMGTEFGQHHEWNHDSSIDWHIAEHPQRRALQDFITEIGRLYRAMP
                     ALWRSDPMPESFEWIDCSDKDNTVLSYLRRDGTDLVIVVLNFTPVPREGYRVGVPIPG
                     RYVERLSSDDLRFGGSEFETLAVVETDPIPAHNRNYSLKLQVPPLGALILSPAK"
     gene            complement(32528..33202)
                     /locus_tag="VFQ61_18500"
     CDS             complement(32528..33202)
                     /locus_tag="VFQ61_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956506.1"
                     /translation="MLSSLGVSALLASSVGACATVEDPVSIGEYGDGGPSPPAGSGGN
                     SVGAGGTTATETTASGGATSNGGTTGDQGGTRASTGGRTTGGRRGSAGSGGRSGSGGR
                     SASAGAATTAGRSSAGSPGSAGSSSGACDWTGASCMNCTQACPTNDGGTCAKQCSAII
                     TCVQMNPGCGTGGDPMCVKRNAMTGQGNKCTNAWDQSGGSSLTPNGPAQLAVSYFECA
                     CGTTVP"
     gene            complement(33489..34448)
                     /locus_tag="VFQ61_18505"
     CDS             complement(33489..34448)
                     /locus_tag="VFQ61_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956507.1"
                     /translation="MTKLAPSISALLLSLPLVACDFGSSTDTQGSAGSPPVQAGGTPS
                     SSGGATGAGGTTAAKGGATSTSTNATGGSTSTGPSGFGTPMQQGLGSTPERYAKADVI
                     RNGVNYRFMANGWGPGFQSQSVSWSGTSFTVVSMEGKQGGNWEPASYPTTFCGVYSDD
                     QSQSCGLPKAIADIKSLRTGWRWSANGNTNQYNAAYDIWLGDGNTVSTHRSFLMVWLR
                     DPQGQQPAGSKKLNTVQVANVQGTWDVWVGVVGGKPCISYARPEGQDLPELEFDVMDF
                     IRDVKTRSIDAPGSTVLSVAVGFEIWNGPITNLKSEDFYVDVK"
     gene            34681..36240
                     /locus_tag="VFQ61_18510"
     CDS             34681..36240
                     /locus_tag="VFQ61_18510"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET9956508.1"
                     /translation="MPLYLGLDSSTQSLTALVIDTDTQQVVLTRSVNFGADLPEYASK
                     HGFLENPDPKVRRSNPLMWVAALEKLLSDVRASGFDWGTIAGISGAGQQHGSVYLNKP
                     ISEVGGWSTSATLVDQIRPLLSRSEAPIWMDSSTSQECRELAEAVGGPERVTQITGSR
                     PIERFTAAQIRAFYKQDPEAYARTREIHLVSSFMASILAGRSAPIDFGDGAGMNLLDL
                     DQLKWNPEMLEATAPELARRLLPAVASSTQVGTVAQFFVETYGFKSGVPVYAFTGDNP
                     SSLVGTGAVRPGTAIVSLGTSDTVFAAMRNPRVDPRGYGHVFGNPAGGFMCLVCFANG
                     SLARENVAQRVGLDWPAFEKAILDTPPGNSNALLLPYFVPEITPKILKPEARAFGSPE
                     FVEFRAREQMARAVVEAQALAMQRYSDWIGETPDLILATGGASANRGILQILADVFGA
                     EVRTLEVNNSSALGGALRAAQAHSGLAWEKLFEAFVSGGRQLHVKPNTAANYTALSQR
                     FAEEVDRITTV"
     gene            36237..>37291
                     /locus_tag="VFQ61_18515"
     CDS             36237..>37291
                     /locus_tag="VFQ61_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956509.1"
                     /translation="MKRRFVPRAPNRARTLRLVLAGGAIASQLTVSSAGRAQARVGSE
                     VHRTSLSSSSRAPCRVDRGRRTLAIGAAVVPGAVVHGAGHFVACERETAKKLALAEAA
                     GVGALLTSGVLLLLTGASQYVVAPLAVGAVTGGSVFGLSYLADVYGVVADNDQAAPRA
                     ARLRVEAGFSYVYDPHFSYRTFISQGFRLQEGRYWLAPQIMSSLDAHNRRYGARAGYR
                     VLGGARPTQVASISRLAPRSWLDVALGAYDHGYGESGFSLTGVEAALLGHLELSALGA
                     RTLRGAFAEGELGYARLWTRFDGLPDLPNDQLLARFMFGFYWGRGQALEGQTQFGYDH
                     RRDTLAGGLKPAGIAAG"
BASE COUNT         6673 a        11728 c        11967 g         6923 t
ORIGIN      
        1 atcgagttct accgccgggc cctcggtgct cggatactgg cgcgctacga acacggactg
       61 gagcgtcacg tgagccactc ggacttggcg cttggggacg cacggttctc ggtgacagag
      121 gaggccaagg cttggaacag cattgctcca agctcgctcg ggggctcacc ggtagtactg
      181 cagtgggggg tggccgacgc agatgacgcg ctccagcgat ttgtccgcgc cgggggtacg
      241 gtgattcacc cgttgcaacc cttgctcggt gaacgcatgg cgcgcctagc cgacccgttc
      301 gggcacgtgt ggctccttcg ccagaaagtt caggacctca cggtcgccga gagccagcgt
      361 cagcgcgatg aactgttcga gcgcttcacg cgcacccagc cgcccgagga accagcgcgg
      421 caccccgccg tcggcctcga aacggaggtt aggctaagcc acgaccccgt gagttgccaa
      481 gcaggaattt cgccgcgtga tgggttgtac gagcggtcgc cccgagttca tctggtggtg
      541 gggccggtcg gcgcaggcaa atccacattg gcgctgcagc tggctcgtca acaccgcgcg
      601 ctacgcctga ccctggatgc gtggatgacg acgttgttca gtcgggatcg tccggagcga
      661 gcgctgattc cctggtacat cgagcgggcc gagcgttgca cgcaacagat ctggtccgtg
      721 gcacaggccg cgctggagat cgggaccgac gtggtgctcg agatcgggtt gattcaacgg
      781 gagccgcggc tgcggttttg ggatcgcttg acggaatccg gcgccgacgt cgtgatgtat
      841 gtggtggagg cggataggga ggttcgtcgt cgccgcgtgg aagcgcgcaa ccgcgagcgc
      901 ggggcgacct tctcgatgct cgtgccgccc gacgttttcg aactcgcgag cgatttgtgg
      961 gaggcacctg atgcctccga atgcgcggac cggcaagttg tgttcttgcg taccgatccc
     1021 gcgccgtaat tgcctactgc aagcttcgga gtgcgaacta cccgggggag gctttggttg
     1081 cgaacgagaa tacacgccag gcgtgtgggg ctcccggatg ggtcgaaacc gtaacgtctt
     1141 cgccggcgaa tctgcggatc gcgctatccc aaaagcccag cccgcgcatg ttgccctccg
     1201 aaacgcgcac ggtccaggtc ccgggccacg cgctccacag caaagccgcc gcaccatacc
     1261 cgaccccgag cctccgatac ttccgtacga cgaagaactc ggccacatcg aagaccgccg
     1321 gatctgggga gaccggcgag ccgcgggtcg ccagagcgaa ccccgcaagc cgcccgccat
     1381 gacgaatcaa ataggcaaaa cggccgccgt tcgtgccgga ctcggcggcc ccgaaataag
     1441 acgcgagttt gggatatccg aaacggccgt cttctcccaa ttcgaccttc ggaaacaggt
     1501 cgcagatatc atgaatatag agttcgagaa gattggccag tatgggaacg tcggtgcgag
     1561 acgccaaatc gagggccaca gagctagggt tttccacgtt tgaaccgacc ggattcattg
     1621 gaagaatagt gaagtggggc cgcgtaaaag cgtgccccac atcacgggag cgctgcaggt
     1681 caggagccgt tcgaaatgcg agagagcgcg ctcgggattc ctacagactc actcacccgc
     1741 ggtgccctcg agctcggcgc aggaaaactg cagcacatgc ccggcgaagc tgcctcgcac
     1801 cggggcgtcc atctcggtgc ctgcctcctc tagatccacg gcgccgtccc caatcaggcc
     1861 gaccagggtg aacgtattct gcccgagcac gagcaccgaa ccgctcgtct cgatcccgtg
     1921 gaacagatgg tgcccggggc tgaagcgccc aggtggcatc gagatttcca ccacgactag
     1981 gcgctgatcc tcgagcattc ccatgtatcg aatacgcgga accccgtctc cactctcgtc
     2041 cagacccgcg cgagctaccc aggaaagctc cacgagctta ccgtccaaat tgagagacag
     2101 ctgattggct cccggcaaag gctcctggag acttccaaag gaggtgtcga tactcccgct
     2161 catgtcagtg agcatcccga ggcagggact ggtgaaagcg gtcgtgtttt cctgccactc
     2221 gggagccggc tgctgcagcg ctgcgcgcag caagccaggg tggtttcgca catgctcacg
     2281 ctgccgctcc agctgcttgg ggtcggcgtc cggggccaaa gcagcgattc gatcgatttg
     2341 cttcagcagt cgatcgacat cccacacgga ttcgttcaat tcaccgaggc gcgcgcggta
     2401 cttctcgcgt tcctgcggta aatgatagag ccgattcgcg acgcggcccg cggcatagac
     2461 cgcgatgttt tggttcaagg ggtcgaacgg attcagccct tcgaacgcgg catccacgcc
     2521 ccacggcaag aacacgaagc gttgactcgc gggatcccgg taaacgaaat agttattggc
     2581 attgccgctg tagctatccc aatgtccgag cagcgtctcc atggcccaaa agtctcggaa
     2641 tttgccgaga tccagctgct tgccaatcgt ggaaacggcc tgttcgtccg gtgcttcgag
     2701 ggccgatgcg agagcgcgcg cgtcacttcg gtcgttcgac atctcgttgg ttttgagctg
     2761 gagctgctct acgcgcacgg cgtcgaagtc cgccgcttgc ccctcataca agttgccttc
     2821 ggcgctcttg aaatgccgct tcagaaattg cttcgagatg ctttcgacgt tgctgtaggt
     2881 tcccagatcc tcgccgttga ccacgacatg agccaagttg cagcgcgaag ctgggagccc
     2941 cgcatcactg aacatgccat acgccaagca ttggtgcgtg tgcgacggat cttgacggtc
     3001 attgttcagg gtgaggctgt gcaggccgac atggttttgc ccctccacca cgcggtccag
     3061 gtcgagcttc aatgaggggc gcagcgccga gagcgacccg agaaacccct tctttctgag
     3121 ctgaacgtgc tcgtaacgtt cgccgtcgac gcgcaccgtg gcatcgaaat aggtgtactc
     3181 gtatggctcg ttcagctcca ggaaggacgt gagtgcgcgg ccttgctcac gcaaaacgtc
     3241 ccagtccgcc ggagccagtt cgatctccac ctctacccgg cgagtggcgc tgaaaaacgc
     3301 cgcttgggcc gtatcctgat caggcccgag caccttctgc gaatcgctct cccccgaaca
     3361 accccatagc gatccgccac acaggcccaa ccacgccagc cgccgagccg cggagtagcc
     3421 agtccgccca attccgccca tggctcctat tcctcgcatc ctcatctcct cacctctaat
     3481 tcagtctatt tcagcgccga gcgcttctac tcgagcggcc ggcgctcatc caagcgctca
     3541 agtatctaag cccaactatt tatttgccca gtgcgatcga tggtcttgcc cgaatgcccg
     3601 agtcgtcgcg cggtcgttca tccggcgggt gccgagtcaa ccggtgctca gccgactcct
     3661 agaaacccgc tttccctggt cgtagcgagc atgttggctc cgatttggca gttccagagc
     3721 gccaacccgc tcggcgagcg gccccattgt gtgcaaagtc agggcgaaac cactagtcga
     3781 ggtacagctt ccgggtcaag ccgggcgcac ggaataacat cgacagaact cgtcagcaag
     3841 caactgggcg ccaaacgtgc caagtgcgat cctacctctc gagcagcgta gctcgaatcc
     3901 gtccggagga gcagctcagt cagagctcag tcagatggtt gcttacctgt tcggctcgat
     3961 cggggcaggg ttgactcatc gaattcggac tgccacctga cgttggcgtg gtcggccgcc
     4021 gttggcagtt ccggtggagc cggtccggcg gcgctgctcc gcccgagtgg gtagaactcc
     4081 aaggcttacg agatcgacgg ctttggagtt ggttcgggtc ccgccgggcg acgtcagggt
     4141 gagcacggtc aatccgcggg tgcgcgcggg gagtcggtgt cgattgaacc tgatttctcg
     4201 cctctagcgt gcgcggcgaa atccagttgg aaaccgatct gaaactcgat gttgtggatt
     4261 gggttcgaga tcagtattag cgagaatcga tatttggagc gagctgcgtt gtttcgcgcg
     4321 gcatgcgcgg tggcgcgacg agtcgcggcc gagctcgggc tgcgctcggt gtgagaggca
     4381 acgcgcagcg cgcccgttgt cgcggggccg ccagcggccg ccgtgaaagc ggcggtggaa
     4441 gcttctgcgg gcgtcgtgcg ggtgtcctcc ggtgagaaaa tttctcgctt cgtgagagcg
     4501 ttcagactgc acccaggggc tgtttacccg ctgcaaaacg tctcacgtcg cagcgcgctg
     4561 ggcagttgtt tgcgcgtcga tcgagggttc agttacgaat gcgcgaagcg cgccccactc
     4621 gggtggctcg cgcgagtgcg tgtcttcgct gatcgtggcg gcgtgaggct acccaaacct
     4681 cccgcgccgg gcacgcgagg cgttcgccaa gggtgtttgt cgaacgggtt gtcgaaccgt
     4741 tcctcggagt gggcggcgtt tgcgcctgtg aacaactccg ttgctgaagg ctgcgagatt
     4801 cgtgacgtcg atcggtaagc caaaccagca gccccaccga cggatctcgt cgacccacca
     4861 aagttggaga acacgcgtct gtcgctagcc agtattctgc gtgcgatgca tactgcttcc
     4921 aggtttcgtc acgcttgcgc gtctcgcgct cgtacccgtt accccccgga ggagcttgaa
     4981 gcagtcgtct acacgacccg ccgccgtcgc tcgctcgagc gaacccgcac cttactcgct
     5041 tctggtatgc acgccggaac gagatgcctg gtcgttcccg atcgtcccgg gccgtggcta
     5101 cacaatcggt cgcaatgctg catccgatat cgtgctggac tatccgtggg tgtcgcacac
     5161 tcacgccgtg atttccggcg ctgatccacc gcagattctg gatctcggga gtcgttcggg
     5221 gacgctggtc ggtacgcgcc gcttggaggc cggcacgctg tgcgcgctgc cggtgggcag
     5281 cctggtggag atcggcagca tctcgttgct cgtacaacgc tcggaggagg tgacacgcga
     5341 cgtttgttgg cctcgacata ccgccggtgg tcccgttccg gattccaatg tgcgagtgca
     5401 gcacccgctc ggcgcggccg cgttcgtcag ccgcaaccga gagatgcgtc gcctttatgc
     5461 gatgttgagg caagtcgccc agagcaacgt gagcgtactg attctgggtg agacgggcgt
     5521 aggcaaggag ctgttcgcgc aagccattca ccagcactcg ccccgcagcg cgaacccgat
     5581 cgtcatcttg aattgcgcgg cggtgccgga gagtctggtg gagagcgagc tgttcggcta
     5641 tcagcgcggc gcgttctccg gggccagcgt tcccaagccg gggctgttcg aatcagccca
     5701 tgattcgacg ttcttcttgg acgaggtggg agagctgccc gtcaccgttc agcccaagct
     5761 gttgcgagtt ctcgaaacgg gagacgtgca acgcctcggc gcgctgcgct ccaagcgggt
     5821 caacgtgcgg atcgtggccg ccaccaatgt cgacgtgcag gcggccatcg cacgtgggga
     5881 gtttcgcgct gatcttttct atcgcttgaa tggcatcacg ttgagcgtgc ccccgctgcg
     5941 cgaacggcgc gaagatatcc tcgagttggc ggagtacttc gtggagcggt ccggtgtgcc
     6001 gtgcgcgatc gatcgcgagc gagagcctct tttcacgccg gaggctcgat cggcgctgtt
     6061 cgcctatgct tggcctggca acgtgcggga gctgaaggcg gtggtggagc gcgcggccct
     6121 gttggcgggc ggcggcgtga tcgacgccga gcatctgctg ttcgatccgc cggtgatacc
     6181 gagctggccg agccacgcaa agcgtctcag catgctaccg agcgcgaacg agacgctgca
     6241 gagcgggccc atttccagtg gaccgcgctc cgcgggttcg tcgggcgggc caccctcgaa
     6301 cgggccgcgc tcgagtggct cgcaagtgag ttcgctgcca ccctccaaca ttccgtcctc
     6361 gcgctggccg agcggggagc ctgggcgcct cagtagcgcg gctcctccca acgatgatct
     6421 gacgttctcg agccaagagc tgcctacatc ggtggtgcgc gcggagctcg agcggcgtga
     6481 gcgccgtcgc atccaggagg cgctggatcg gacgggcggc aaccagaaga acgcggccat
     6541 cttgctcggc atgtcgcgtc gcacgctgct caagcgcttg gaccgctacg agatggctcg
     6601 cccgcgtaag cgccgcgagt aagggcacgc gcgcgcatcc gggggagtcg cgaggcggcc
     6661 gcaaggcggc cgtccgagag cgtgccgatc cattcggtag gtcgaggtca gtgggacgag
     6721 ggagcgtcac gacgagctga ttcgccctag cgagcgaagc ggcgctcggc gtctcgccta
     6781 tttccgctcg cccgactcgc cacggggaca cgaagcgcag cgcggtgcgc ttcgcgtcgc
     6841 ggtccaaaat gcgtgagtgg gcgtgggagc cccgttttct gagcttgcaa acgaaagagt
     6901 gtcccgccct ggagcgctcg tggcgtgcgg cactgcgatt gcaatgcagg cgagtataaa
     6961 gcggcggacg attgcaacga ctattgcagc ttcgcgcaga gcagtaggtg cgcacggctg
     7021 caacggccgc atgccatagc ttgcagtaat tagccccggc gtggtgctcg cctcgggctc
     7081 ccgccatcac cgaaggagat gctgatgata tcgagacgac ggttcgggtc gcactgtggt
     7141 gttgctggtt cgttcgtgtt ggctatggcg tgtcagagcg tcccggacgc ctcggatagt
     7201 tcggaaaccg tcacgttgac attacccaat ggggcctgta atcaggctca accagaagat
     7261 gtgttgatcg ctcagctcgt caaggacagc tgggttgcgc agtatccact cacacgattg
     7321 ttcgtcgatt cgagctcgaa cgtcacgggc ccgagcctgc cggcaatttt gcagttcgat
     7381 ttggatgtga tcaatggtgt gccggccgcg aaaacggccg tcagcgccgc ggtcgccacg
     7441 atatcgggtt tacctgatta ccagccggac ggctttggcc cagaaacgcc ggcgtgtagc
     7501 gccgtccccg cctggacgcc caacggcata acccaggttg caaccaccga agtggaggtg
     7561 ttcacgacag ccgtcaacca agattcttgg cgaaaaacac acaaggcctt cggcaaagag
     7621 tgcccgctac tgcggcgcac ggggaatcag gacctcttgg atccgcccgg cgacggcagc
     7681 acgaatatgc cgccgggcgg cacggtaagc gccacgggcg tgaccgcgaa tgcatacggt
     7741 ttgtgtccga cggggaccgc cccaggtact ttttgtaaat tgagttatgc gacgggcgtc
     7801 aattacatgg gacgcaagtg tctgtactac tacggctctt tgcgctgctt gctctactga
     7861 acgtgcgctg aagtcgaata tggactgaag tgcacgggcg agttcggata ggtgtgggat
     7921 tgcacgcaag cggtgtgtag ttcgacgagc cgcgatagtt cgcgtttggg ctcgagttcg
     7981 aattccacgg ttcgcgacta aagggttgat cgcgagtccg agagagtagc gttaggtgga
     8041 gactgtcagg tgagcgtacc gccgcgttgg cgggaatcct aaatcagcgg gatgacagtc
     8101 gagcccggtg gatcggactg aactcgcgtt gccatggtag aggcccgaga ttgagggagc
     8161 aatgaactac aatcgttact cgaggtgtga cttcactggc ggtttgctgg gctgggcttc
     8221 ggttttggcc agcatgacgg cagtgcttgc gtgcgatgat gattcgggcc agcccgctct
     8281 gacccaatgc ccaaccggcg gcggaagcgg gggctcgtcg agtacctcgg atacgagcgg
     8341 tggtgtgagc gaaggggccg cttcagggcg caccaccgct tcggccgagg gtggcacgac
     8401 gagtgctggg acgacggtct catttggcgg aaccagcgcc aaagatccgc agacgggagg
     8461 gtcgaaggca atgggtggag tcacgcaagt ggccaccgaa cccgaaggtg gagcgggcgg
     8521 taccgagccc acgacggacg tacctacgga aggcggcgct ggtggcggca cggcgacgac
     8581 cgaaccgaac ccgccggaca cgaactcctg gacctccatc tgcgaagaca acatgggtat
     8641 gttcctgccg tacgacttct cgcgggggat cgcgggggag tatgcgttcg aactctcggt
     8701 gagttgtgac ttgggtgggt acttgttgcc cctggtggtt ttggaccctg tgaacctgac
     8761 gggtgtcgac gcttgggtga cccagctgac ggattggctg cgtgggcgag tgctggtgtg
     8821 taacgacgat ttcagcaagc tgccccccga cgacttctcg atcctgccgt cctcacagcg
     8881 cgcggggatg tcacgcggag atttcaattc atccgtccag ttgttcttga gtgtaatggc
     8941 gcgccacgat ggtttgccgg atggtatcaa tccagaattc aagacggttc tccaagagcg
     9001 tctggcgtct tatgccgaga gtacggtgtc cgtgcagagt gacgccctga cgaaacaact
     9061 atcgcccccg gactgtatcc caccattata aagtatctgc cgtagaacga ccgttgcgcc
     9121 ggcgcgcgcg taaggatgcc cgggtatgat cgcccaatct gcccgggcag agattccgtt
     9181 cttggatgcg cagcgcgcca gttgcatttc gcggactagc agcccgaacg cgccgcggag
     9241 tgtgcgaaac ttgttggagc gatccatgtc ttatttccac ggcttgtccg aaacaatgca
     9301 gtgcttggct cgctccgcga cgcgagccat cgaggcgagc gtgtgcgcag cgaacgcgcg
     9361 gaagcggcgc cagcgacatg gcacgtgggt ccacggcgta acggcgcagg tgctattgcg
     9421 ggtcacggca gtggtgattt gggccggagt gggcggctgc acttacctgc tcgacttgga
     9481 acccaatcaa tgcaaagtgg atgcagattg cctatcccgc ccggggttcg aaggcacgag
     9541 ctgcgtcgcc ggttcctgca agacttcgct gtccgccgcc gggtccaccc agggccctcc
     9601 gaaagacggt tgtacgagca acacgaagtg cgtagctgac cacaacaatc agccctacgc
     9661 ttgcatcgag cccgggcaag attgcgtgcc gctcacgacc gaagagtgtc ccgtcgtgtt
     9721 cgggaactac aacgatgaca acgcgctcta cttcggggcc ctgctcaacg tgccgagcaa
     9781 tgcgccactg tctcagcagt cgaccctgaa cgttcaactc gccgtcgatg agttcaatgg
     9841 ctcggtgggc ggcctcccgg gcgggccgct cggtaagctg aggcctttgg tgggggtggt
     9901 atgtcggaac gagccagagc tcgtgccggc cgaatcctcc cacctgttcg atcgtatcgg
     9961 ggtggcggcc accatcgctc acttgccgag cggagaactc aagagcttct tggtcgatcg
    10021 ggcgctgccg aagcaagcct tcgtaatgaa cccggggttc gcggacaact cgctcacctc
    10081 gctctcgagc caaggcttgt tctggcacgt gatcggcgat attcgcgatg tggctccggc
    10141 ctatccaccg cttgttgccc ggttggagaa gatgatcaag gcgggcggct ccgacgagcc
    10201 cttgcgggtc gcgatgctcg tgagccggcg ctacgccgag cagagcatcg ctgacacgtt
    10261 ggctgcgcag ctcgagttca acgggaactc ggcggcagag aatgggagtg ccttctacac
    10321 cgccgcggtg cccgtgctcg cggacgagcc gtcggcggac tacacgtccg tggtggacgc
    10381 gctcctgaaa tttcgcccca atgtggtgat cgcgatcacg cgcgaagagc tcgtgtacaa
    10441 gatcctgccg gacgtcgaag agcgatggcc gcgcgatcag ctcgtcaggc cttactatat
    10501 tctgcccaac gcactttccg gtaatcagaa tctactcgac tacgtggcgt acgactcgcg
    10561 cgcggtatcg agcgaaaaca agcgacaacg catggttgga gtggcgcccg cctccgccga
    10621 ggacctccag ctctacaacc aattcctact gcgttttcgc gcggcgtatc cgacattccc
    10681 gaatccgggt ggattcgaga acttctacga cgccgtctat ctagtcgcga acgcgatgta
    10741 cgccgccggc tcggtgccgc gcctgactgg gcccgacgtg gcgcgcggca tgcaaagagt
    10801 catcgcgggg ccgatcgtcg tgaatgcggg tcccaccttc atcgccgatg gtttcagcgc
    10861 gctagcggcc ggaggcaaca ttcgtctgaa cggcacgatg ggtctggctg atttcgatcc
    10921 aggtgtcgga gcccggcgcg gcaatggcag catctattgc attcagcgtc gagggagcca
    10981 attgggcttc gtctacgacg tgttgcgcta cgacaaatcg agcaagacac tcaagggcga
    11041 gttcccttgc tttccgggac tttagcggaa ccacagcgcg agacagcagg cgttcatcca
    11101 ccgaatgggt cagcgaacta tgtacaccgc cgagctattt cccgatatga ccgacgaacc
    11161 gctgcaagca ggcgatctcc tgggcaagta tcaactggtg tgcccggtcg cgcgaggcgg
    11221 catggggcaa gtttgggctg cgcgtcagac cggcttgctc ggactgcccg gtttggtggc
    11281 gatcaagacc gcgctgccct cttcggaccg gacgctggag agtatgcaaa ctctgctgtt
    11341 cgacgaagcg cgggtggcgg cgtccatcga tcacccgaat atatgcaaga tcctcgagct
    11401 cgggttcgaa cgcggtgtgt tgttcatcgc gatggagtgg atcttcgggg tgccactctc
    11461 gaaccttctg agcctggtgc ccaatcgcaa gctggattgc tctacggcgg catacatctt
    11521 ggcgcaggct tgccgcggct tgcacgcggc gcacgagctg accgacgaag agggccaccc
    11581 gctcgaggtc gtgcaccggg acgccacgcc gcacaacatg ctgattacct tgacgggtga
    11641 tctgaagatc gtcgacttcg gtatcgtcaa ggcgcgcaac cagttacacc aggtgacgga
    11701 gaccggcgag ctgaagggca agatatccta tcttgcgccc gagcagctgc gcggcaaagg
    11761 tgtcgaccgt cgctccgata tcttcacgct gggttgtgtg ttgtacctcg ctaccaccgg
    11821 gcgagccccg ttcaacgggc tcgaggcggg gacgacggta atgcgcatca tgaacgggga
    11881 gtacgcgccg ccgtcctctc tggtcgaaga ctacccggag cagctcgagc aaatcgtgtg
    11941 tcgcgcgcta gcgctcgacc cgaatgcgcg ctttcaaacg gcgcaagaaa tgcaatacgc
    12001 gctcgaggca tttctgaacg gtcaagacgg ccgagcggcg gtggcgcgca cggtcgagga
    12061 gtattgcggt gcgatcgtcg agcagcgccg cgcggatata cgcgcggccc agaagctctt
    12121 ggattcccaa gccggtcata tggcttcggg tacgtatcga accggaacgc gctctccaga
    12181 gtccgaggga tcctggcgtg aggaagctct gcggttgctg tcgaccgcag ccaagacgcc
    12241 ggccacgcaa tccgaactcg tgccgactca ggccgcgcgt agccctcgtc atcgctggtt
    12301 gatcgggggg agtgtggcgt tggtcgggtg cgtaggtgcg ctgatcgtgg cgagtcaact
    12361 gcagcgttcc gatgcgcgtc gcgcgtcaga aggcagcgcg ctgcgggtgc cgagcgccag
    12421 taacgtcgcg gcggcttcgg tcacccccgc cgcgcgcgcg tccacggccc cggcacgcac
    12481 gacccgagag cccatcaatc tcacgctgca gtcgcgcccc gaaggcgcga aggttcgggt
    12541 cgatggaggc cccgctcaag cgaccccgcg tacactgcag gtgccgcccg acgacaagga
    12601 acacgtcatc acgttgcaag cgcctggcta cgaggatatc cgccaaaccg tacggttcaa
    12661 ccgggatcag gtcttccact tcgacctgtt accgaagcgc gtaacggccg gcggccgggg
    12721 gctgccgcgt agtacggacg cggggctgac gaaggcccaa atcgcggccg cggctcgggc
    12781 cgccccggag cgcgcgcggc agagcaacga gacgtcggct ccgcaaccat ccactcaact
    12841 cgagccgacg ctgcagccgc tgctgcgtca ggcgccgcga catcagattg atcaaacgga
    12901 tccattcgcg aggcaacgct gatgtcaccg acagccagag ccgtggcttg cggtctgatc
    12961 gcgacgcagt gcttactcgc gcgctcggcg ttcgcggatt caccgccgag cgaagggccg
    13021 tcaggcgctc ccgcgggcga gcccgtgcgt gtggagaccg acaggcctgc cgccgatcgg
    13081 gcggtgagca atccggagcc cgacaaggta gagctcgcgc gcgttcactt cttgcgcgcc
    13141 gtgcagttct tcaacgacgg tgacttcaag ctgtcgctgg tggagttccg gcgcgcttac
    13201 gaaatctccg gcaactatcg cgtgctctac aacatcggtc aggtcaacca ccagctcagc
    13261 aactatacga aggcgctcac gtcactcgag cgttacctgg ccgagggtgg ggatgacgtg
    13321 ccggaggatc gtcggcaaga ggtgagtcaa agtatcgtcg agctcaacaa gaaggtcgct
    13381 cgggttcgct tgatcaccaa cgtggatgtt gccgaactct ggctcgatga cgactcgcag
    13441 ggagaaattc accccgggca agtcgtggtg attgacgcgg gcgaccaccg tttggagttg
    13501 cgacgccagg gctatcgctc ggcactcacg caggtcagct tgacggcagg ggagaacagc
    13561 gacctgacgt tgaacttggc gagggcgccg cggcaaatgc ccatgcccgt gcgctacgaa
    13621 ccgccggtgt cacgcggcat gccggcggga gtctggttgg gctggttcgt cacgggcgcc
    13681 acggccgtgg gcgcggtggt gacgggggtc gtggcgcaga aacatgccaa agacttgcat
    13741 gatttgcgtg actccccgac gtcgacctcg gaggagcggg agaacgcgcg cgaccgcgcg
    13801 cgcagcttcg ccatcgcttc ggatgcgttg acgggcaccg cgctcgcggt gggcgcggtt
    13861 tcgctctact tcactctgtc ggtgagcgcg gcgaatcgcc ggggaggagt ggacccactc
    13921 actcagctcg cgctgggccc cggctcgttt acgttacgac gtcagttctg agcgcgcata
    13981 ctcgaaccgg aaggagcggc gcggcctcag cgtagcagcc cctcaatcgc gagcggctcg
    14041 agctgggccg aatcgggcag gtcgatttgc gccttttgaa ggcgacccga caagtcgacg
    14101 ttgacggcct gccagcgggt ataagcgtcg agcacccaga tcccactctc gcgcgtgccg
    14161 ttgccggacc agccgttgcc gccgaagggc aaatgcgctt ccgcgcccgt ggtcgagttg
    14221 ttgatgctgg tcatgcccgc ggagatctct tctttgaagc gcaacataca gcgcgcgtcg
    14281 cgcgtgtaga tggcgctaga gagcccgtac ggggtgccgt tggccgcggc gagcgcttcg
    14341 tccacgccgt ctacttcgac caagttgacg gtggggccga agcattcctc ctgggcgatg
    14401 cgcatgttca tcttcacgcg gtcgaagatg cgcgggctgg cgtagagacc tttgctggcg
    14461 tctccgctga agttgggggg ctcttcaccg ggctggatct gtttgccgtc tagcaacaac
    14521 tccgcgccgt ccttcaaacc cgtaccgcgt tggagcaacc attgctccag gaatcgctca
    14581 ttgatcagcg gccccaagtc catgcgctcg ttgctcggat ccccgatgct gagcgcacgg
    14641 gcacggcgca cgagctcgct gcgcaccacg ggcatcacgc gcttatcgac gatgatgtta
    14701 ccggcgctgg tgcaacgttg cccggcggta ccgaaggcgg cccacactgc accgtcaatc
    14761 gccaggtcca gatccgcgtc cgccaagatc acgagcggat tcttcccgcc gagttcgagt
    14821 gagggcacct gcagcgcgcg cccacaaacc tcgccgatct tgcggcccac ttcggtcgag
    14881 ccagtaaacg agatcttgtc cagcagccct tgctcgacgg cttgcaccaa gaactcaccc
    14941 gcatgacccg cgccggcacc atgaacgacg ttcaatacgc cctgcggaag cccggcacga
    15001 cgccacagct ccaggaacaa cgtggcgatg ccgggcgcat cgtccgacgg tttccagact
    15061 acgcagttgc cgcacagaat cgccggaatg agcttccagg cgggcaccgc gatcgggaag
    15121 ttgcccgcgg tgatcacgcc gaccaccccg agcggtcgac ggtaggtgaa tagttccttg
    15181 tttgccagct cactcggcac ggtttgcccg tagagccttc gcccctcgct ctgaaagaac
    15241 tcagcggtat cgatggcttc ctgcacgctg ccccgcgcct cgcgcagtgg cttgcccact
    15301 tcgcgcgcaa tgaaccgcgc caggctctct ttgctctcgg tcagcaactt acctagtcgc
    15361 ccgagcactt gagcgcgctg cggcgctggt gttcgagccc acccgacttg cgcggcgcgc
    15421 gcggcttggc aggcggtgat cacctcttcg cggctgcttt gcggcgccaa agtgaccacg
    15481 tcatcacgaa atgctgggtt tcgtcgggcg agcgcgttca agcccgcggg ggactcgcga
    15541 ccaccaatga agttgtaggc gatcaaagtt tgggcgaggc cggcgctttg gacgcgggtt
    15601 tgcatccagc tagcgtggcg ctgccctcgc tgaatcgcaa gatgcgtgtg cgcgcagata
    15661 gcgctggtcg agctcgaagc agcgggtaga gcttcgcagc ccacgaactc cagagctgca
    15721 gttcgagagc actgatacgc cccgcagcgc cgtgctcccg cagcgccgtg ctcccacaac
    15781 gccgtgctcc cacaaagccg tgctcccgct cgaggcttcg aattcgaagt ggcaccatct
    15841 gcagactgga gcggacagtc ggcagcaccg cccggtgcag cgggcgcgag gtgcgacctg
    15901 cgttcgtaac ggaaattcgc gggctcggtg tgactcaaac atgcagctca tcacatgaac
    15961 cgctttccac gtggtgttcg cggcttgccg agcggtggcc ttcgtgttcc tctccgagct
    16021 cggctcagca tggctagaag tcaattctta ccggctgcca tcgcagtggc ttgttatgcc
    16081 gcagaagcgc ccaagcctgc tatccactcg agtgccagct tggcgagcct gattgatgtc
    16141 ccttcgctgg gggccgcacg cgcgccggac gcggcagccg gcacccctcg gttgaagtcg
    16201 ggggcgctgg tagcggcagc gcccggatcg cagcagttgg cgtcggcgac gcttggcgcg
    16261 tccagccccg tccgatcgct ttgcgcgccg gaaatggcgg aggtgcagcg gccagagggc
    16321 acgtattgta tcgatcgttg ggagggcagt ctggttgatc gcgcgggggc accctggccc
    16381 aacaaccaaa gcctggataa gccggatgcc cccgccgggg atctgatcgc cgtgagcgcg
    16441 ccggggcggc tgccgcaggg ctatatcagc ggagcccagg cggcacgggc ttgcagaaat
    16501 gctggcaaac ggctgtgcga gttggacgag tgggcgcacg cttgccgagg ctctcacggt
    16561 tatcagcatc cgtatggcaa tgaacggcgc gtgggggcgt gcaacgagcc gcctcggcgt
    16621 agcgctggcc acccggtcat gatgctgttc gatcgcttcg cgaagcccgg caccgatcgc
    16681 gcgacgatgt ggaactccga atggatgaac gatccacggc tgcacgagat gccctttggt
    16741 ctagagccca ccggctcgcg cacggaatgc aagagcgaag caggcgtgta tgacatggtc
    16801 ggtaatctgc acgagtggat cgccgatccc gaggggacgt tcgtcggcgg gttcttcctt
    16861 gacactacgc tgaacggcga aggctgcgcc tatcgcacgc gagcgcatga tttcgactac
    16921 cacgattact cgactggatt ccgctgctgc gccgacgtga gcgaaggcgc gagcgccgca
    16981 tgggctcagc ggcgctaata acccacgttc gtcagctggc cgcgtaagca cagctcgagt
    17041 tcacgtcatc actcagggca gcgggagcgc gcacgcgtcg cgccgagcgc gctcgccagt
    17101 cgggcacagt ccaagagcgc gtcgggcatg cggcaggaac aagtgccgtc ttgctgctcg
    17161 aaacagcagt ccgacgcagc acaggaaccg agcaacccgg gcgcttcgag ctctagatcg
    17221 acgtggacgc aagagcacaa gcctgcggct acgccgcacg accaagctcc gccgaagacc
    17281 gagctagcgc ccgcgttatt ggctcccgcg ctaccgccca aagctcccgt tccgggctcg
    17341 ccaccagact gggcaggggc gccgccttgg gctggcgttc cgccattctc ggtcgtctcg
    17401 cctccaactc cagcgctggg cccgctggtt gcaccgccgc tggttccaat ttcagggacc
    17461 gatgcggggc ggtgatgtgg gcggttactc gtgggcagaa agcgcgactc gccttcatcc
    17521 ccgcaagccg aggacccgcc ggcgccgatc gcaatagcca gtgacgccag gcctagttgc
    17581 gccctcaatc caccgcctac agccatgtga agcactccga gaggtagccg cgcgaggtgt
    17641 ccaccacgat gtgctcgaag ccgtccaccc gcaccccagc tcgctccagc gtggcttggt
    17701 cgaccgggct atagagcgtg tcatcgcccg gacctccgtc ccagtgcgct tcggactcga
    17761 gctcgcacgg gtgctcgatg cgcaaggcgt cgtcaccatt acccagggcc acgaggcggg
    17821 tttctgggcc ggcaaacgcg acgtcgtccc ccgcgcttcc gatgagaacg tcggcgccag
    17881 tgcccccata tagaactgcc cgcgccgcgc tctgcgcgtg aatgacgtct gctcccgcgc
    17941 ctccgaagag catgacattt ccgccacccg cagcggtcaa atagtcgttg ccggggcctc
    18001 cgaggatgac gtcgttaccc ccgcgactcg ccaaatagtc gtcgttcgtg ctgccccaaa
    18061 tgaagtccga attggcgctt ccaagcaata gatcagcctc gtcgctgccg agccacagcg
    18121 tcgcgttcca ggcgatcgcc gcgggcgcgt ccggttgaat cgacatgacg aactgatccg
    18181 cagtcgcgca gcgggcgctt ggcgtgtgcc gtagctccac gccgacctca cgagttgcat
    18241 agagcttgcc cccgctgtcc cgcactgacc aagtcaatcg gtgcgagccc atcggtagca
    18301 acagaagctg ttcttcggtc gcatcgcgcg gctccgccag ggtatgccca ttcacctgga
    18361 ccagccgcgc gcgcagcagc ggcggctcac aggcgccgtg cacctgggga ggcacgagtt
    18421 gaacgctttg tggttcgggg gtgcagaact ctagatattg cggagagggc tcgtccacga
    18481 cgagcggtgt gccctcgttc tccaagccgg tcggacatgg atcgacggaa tcagccagcc
    18541 cgtcagcgtc cgtgtcgggc tccgattcga gcgtctgaaa agtggagctt acgttcgaat
    18601 tcgtacagcc cgcgctcgtc gctcccaggc ctccgatgag gcacagcagc catacactcc
    18661 gcgtgagttt gggtctcacg cttctgagtt gcccgatctg cgcggtcggt gcaagcgcgg
    18721 caaatgcgaa agagaccttc gtcggctccg ggccatgaag ccaaactctg cacagcctcc
    18781 ctgcatcgcg cccgcgtttg cgctccggcg gccgcgcgga tgaccgttct gcggcggggc
    18841 gacacacgct gaaatattcg cgttgcacta acggccacgc atcgacgtga gcaaccggtc
    18901 gggccgtacc cgctcgggcc gtactggggt ttgcgacctc gaacgcgcat gattattcgc
    18961 aatggccgag ctcgagcgaa ttggattgag ctcagttgat cgagtgcggg gcggccgtat
    19021 gcgccgcgga ctcagcttcc ttccggtgcc cttcccgctc cgccagcgcc acgtcccgtt
    19081 gctcctcggg agtaagccct cgaccgtagt gtttagcgta cacctgcgtg aactcggccc
    19141 cgtaaatgaa catctgcgcg gaaaagtata gccaaagcaa gaccacgatc gccgaacccg
    19201 ccgcaccgta ggtttgggtg acgctcgccc ggcccaagta aaggccaacc agcgagttgc
    19261 cgatattgaa cagcaccgag gtcagcagcg cgccgcgcgt gaccgcatgc caaccgacgc
    19321 gggtatctgg tagcagcttg aagaccacgg ccgaaattgc cgcgaccaac agcaacgaga
    19381 atacgaactg cccgagctgc accaacactc cggcggcggg tgacgacgca aatagaacct
    19441 cgtggagccc ggtgagcaga gtgcgcgaga gaaacaccaa cagcagcagc ggccccgcgg
    19501 cgatgaccaa caacaacgcg accgcacgac gtttgacgta gtctcgaacg ctgtccttga
    19561 agccagcggc ttggtacacg tctacgttcc aaacctggtt cagcgcgtca cgcagctgcg
    19621 cggtcatgcg agaggcggtg taaatcacga gggcgaagcc cgcgaccgag gccacaccct
    19681 gcgaatcgga cgcttcacgc acccagccct cgatggtcgt cgcggctttg gtgcccatcg
    19741 tctgggttag catttcggag acttctcgct ccgcggcccc ccgtcccagc accgcacccg
    19801 cgatcgccac cgcgataatg atgaaaggcg cgatcgacat cagcgtgttg aaggcgaggg
    19861 ccgccgcgat cacgtcaccg cgatccttcg agtactcgtc caaggtctcc tcgatcagac
    19921 gcctggcggt tcccacccag gccggaagct tgaacgggcg gcgcagagtt tggctggacg
    19981 gcaccagacg agctactgcg agattcgtac cggtgagctt cgcgcaagag accgagacat
    20041 cacgcgccgg atggccacgg tggggcatga gtgagcgccc cgacgctcac tcatttagaa
    20101 ttgagggtcc cgctcaggtg cggccgcgct cctcggatgg ttgacgcgtc gaggtcagat
    20161 tgcgtgcgct attgaccaag gcaacgtgtg agaaggcctg cggaaaattg cctacgaggc
    20221 gcccgttcag ggtgtcgtac tcttcggaga gcagtccgac atcgttcgtc acggccacga
    20281 gccgctcgta gagctgttgc gcgtcatttc gacgtccgag gagtgtgagc gcgtccacga
    20341 gccaaaacga gcaggccaga aacgcgccct catggcccgg gaggccatcg acgccgtccg
    20401 aacgatagcg gagcacgaag ccattctcga tcagttctcg ttcgatgcgc tgtacggtgc
    20461 cgatgacccg tgcgtccgtg gcgggcaaaa aaccgacggc agggatcaag agcaaggcgg
    20521 catctagccc gcgacctccg tagtattgcg tgaagctgcg cagctccgaa ttccaggctt
    20581 ggcgacacac ttcttcgtga atgcgctcac gcgtggcgcg ccaacgttcg aggggacctg
    20641 gcagcccaaa ctgctctatg gccttcaccg ctcggtcgaa agcgacccaa gccatgacct
    20701 tggagtgagt gaactggcgt cgtggtccgc gcacctccca gagcccttcg tcggccgaag
    20761 accaattgga ttcgagccac tccgtcagcg ctcgttgcag cggccaagct gggtcgcggg
    20821 gcgtcattcc cgcgcgacgg gattggtgga gggtgtcact caactcgccg taaacatcga
    20881 gctgcagctg cgccgcggcc gcgtttccga tgcgaacggg tctcgattct tcgtagccga
    20941 cgagccaatc caaggttcgt tcttcgagcc gtcgttcgcc ggaaattccg tacatgattt
    21001 gtagtttgga gggatctccc gccgccgcgc gcaatagcca atcccgaaag gcgcaggcct
    21061 cttgttcgta gccaccactc atcagcgcgt acagagtgaa ggtcgaatcg cgcagccagc
    21121 aatatcgata gtcccaatta cgcactcctc cgatcgcttc gggtaaggac gaggtgccgg
    21181 cggccacgat cgcgccagtg ggaccatacg tgagcgcctt gagcgtgatg agtgagcgaa
    21241 cgatcgcgtc ccggtattct ccgctgtccg tgcagtgtga aacccactct ctccaccatt
    21301 cttccgtctc gcgcagagcg tggaacgggt cgggtgattc cggtagggcc tgatgcgaag
    21361 cgtgccacgt gagcacgaat gggacacggt ctcccgcacg gatctcgaaa tcagcgaccg
    21421 tggtcaggcc ccttccgcaa tgcttgatgg gagtatcgag ctgtagcgca tcggggccag
    21481 caatcgcgtg caggcgcccg tcgagattgc ggacccaagg tacgatactg ccgtagtcga
    21541 agcggatgat tagctccgat tgcattggca cgcagccgct cagcccttcg acgatccgca
    21601 ccacgtccgg gctctgaccg cgtggaggca tgaagtcgac gaggcgaacc gcgccatctt
    21661 cggtctcgaa cgtggtctcg agaatcaagg tgtcttgtcg gtagcgtcgc gcaaccttgg
    21721 gtgcgaccgc cgttcgctct cgattgacgt gcggcgcgat ccgccagcga ccattgtcag
    21781 gcgttcccaa gagagcggca aaacacgctc ccgaatcgaa gcgagggaaa cacagccagt
    21841 ctatcgaacc ggctttggac accaacgcca gagtctgagt gtccccgatg atggcgtagt
    21901 cctctatgcg cacgcaccaa catgcacgcg ccgattggac cgattattcc cggtcgtgct
    21961 cgcggcgagt aagtgggtgt ttccgcgcgc cgggctgggg gatattcatc agctccgaac
    22021 tgagtcgttc atgaacgatg acaaaaaaga aagggccgct ctgaagcggc ccttttggca
    22081 cactcaccgt tctagcaact gccgagtcgg gtgccgtcgt gccgaatcag gtggtggtgc
    22141 cggccgtgcc gcccgcgccg ccgcccgcgg tgcctgcgcc ggccgtaccg cccgcgccgc
    22201 cgcctgctgc gccgccacct gccgcgccgc cgcccgccgt gccgcccgcg ccgccgccac
    22261 cagcgccacc cgcggtgccg accaccttac acgcggcctg cgtgccaccc gtgagggtga
    22321 tgtcatcgat ggtgatgtcg accggataac cgtcggccat ggtggccacg ggcgcgaact
    22381 gccactgcaa gccgagcatt tccttggtgc tcgtcgctgc gctccacgcg gtggggccgc
    22441 cgccggagat gtctgcccag gtcatcgtga ctggcgaagt ggccgttgct acgtcgatgt
    22501 cctcggtctt cttggctgca acacagggac catcccacgg cttggttgcg tccgtcgcct
    22561 tgcacgagcc cttcatgtct gccgggttgg cttcgtacgg ataggtggag ttggtctgaa
    22621 gctggaactg caccttggcg cccgcggttc ccgtgatctt gaacgataca ccggtgtaag
    22681 cggaaacatc ggagcacgag ttgaaccaca cggtgatgcc gctataggtc ttgatcgtgc
    22741 cggtcaggtg cagtgcgccc gtgtccgtgc cttcacgctt caaatcgccg tacgtcgagt
    22801 agccaccgcc gaagtcagcg ctgccccagg tctgcgcggt ccccgtgggg aactcggaga
    22861 tcgtgctgca gtccgccact ttgccggcgc acgcgaaccc ttgagcggtt gcgccgcccg
    22921 tggctccgcc gccgccaccg gttgcggtgg tgccaccggt cgtgcctgtg gcgccgccgg
    22981 tcgtgcccgt cgcgccgccg gtggttccgg tgctgccacc tgttacgccg gtcgcgccgc
    23041 ctttggcagt gctcccgcca gcggcgttgt tgttgctgga gctgtcgctc ccgcaggcgg
    23101 cgaacacaaa aataccgcaa atcgcggcaa aaccaaatcc accaatccaa acacgagacc
    23161 gcatgatcta agcctccaag ccctgtgggc tcttcgagtg accagccctg tgggctagct
    23221 caggtaactc gccatcggga caagctgagg tagccctccc gccgggcgta ctgggtaaac
    23281 aggtcagtaa aaacacgtcg aagcgcgtaa cggtaacggg accgctaccc tatagttgag
    23341 tttccgttcc cggccaggca ctttgtgtgg gatctcgaat gacgcggagc tccccctgat
    23401 cgcgatccac cggtatccgg ctgggacggc ggtttggcat gcctctcgag gcctgcacga
    23461 gcccccaatt ccaccgtcct gtaaccgaaa ctgcgcaaat ctcgacgcga gcggcgcgaa
    23521 gtgcgactga gaagtcgcgg cgctttcagc ccgtgtcggc aaagccccag tctacggccc
    23581 gggtcgccgc aagcacgagt acacgggttc ctgtacgggt gagcaagcta ctgctgcgag
    23641 taaactctcc acgctgacgt tgccgctcca gaaaggcatt tgcgctcgcc tcctgcgcaa
    23701 attcaatctg ccaagcgacc aggacgtggc tgccgctggg cgtgccggcg tcagttggac
    23761 tgccgccgct ctcgctgcga ctggtaaaga ctttcagttc gtctcctcgc cacgcgagag
    23821 cgatctcgag cgcagcgtcg aagtccggca cggcggacat gccccccgcg ctcgacatgg
    23881 cagccagtac accgagtgag ccgagctcgt catcgccgac caactcccat tccgcgccgg
    23941 gcgcgagtcc agcaaaggat gctgccggca gctctggggt gtggcctccc atcacggtca
    24001 aagcgtgctc gggcgggtcg agctggagct caaccacacc tgcgtgcttc cgcttttgcc
    24061 acgcttcgct aacgaagcgc ccaccccaaa agtaaaccag cgtacgccgc agggattgta
    24121 gcggcgtctt gccggcgacc aactgctccc ggctgctgcg ttcgatggag ttctcgaaat
    24181 aactctgcca atcgaccttc tcgggatcga ggcccaatgt ggccgccgag tagcgtacat
    24241 cgagccaccg cgcttcgccc tcgatcacgg cttgcgcggc ccaactcgcg tccgagctac
    24301 tcgaatgtgc ttcggagaag tcgttcaggc cgatctctcg atcctgcagc gcgtgcacga
    24361 actcatgaac cagggttggg ctcaagctgt gccactcgag cgattgcgca taaaggccct
    24421 ccccgatgat cgtccgcggt tggggttggg acgtgcgatc gaggactacg atatcgccct
    24481 tctcgggacg ataaacccca cccaaccaac tcagctgctc ggcgacgtag gcatcgctct
    24541 ccagcgctcc cggcggcatc agcccgagca tggcgagcgc agtttcgtag ctctttactt
    24601 cggtagaggg ctgagctggt aattccgcgc gcagctcggc cgcgtacgcg gtcggtgtca
    24661 tgacgctgat tggaggcatg gcctcctcgg caccgccgcg caaacaacga gcgatgtccc
    24721 acattcggcg ctgacactcc ggggttccga tatcgcatat cgatgtatcg agttctggac
    24781 attcggtcag ccgtgaagcg ggtgaatgcc cgagctcggc ggtcgtctcc tgcgtgctct
    24841 cgccgcaacc ggcgagcccc agcgcgcagg ccagcccctt cgcaagtcgc aacgcgtgcg
    24901 caccgaaact ccgtctcgtc attcgaaaac cgcctcattg cacgccttgt attccgaaca
    24961 ttcctcgcgt gctcggcagg catccagagc gctgaactcc gcgtcggacg caatgctcat
    25021 ttccatttcg cacacgtccc ggggatacag ccaaccgcac tccaaataca gctccacttg
    25081 cagcgcgcag aagtcctttg ctctttgcgt caccggcgcg cgatagagct cctccaagaa
    25141 gcatggcttg aactcctcgt tccagagggt ctgatcatcg agtgctgcgc agctgagcct
    25201 ctgaacgcac tgcccgtagg cttgggccgc gtgccgtgga attgccgaaa ggctcgaatc
    25261 ctgcgtgcat gccgcgcggc aattggcgcg gtccgggttc aatccacacc tgaaagcggc
    25321 ctcgcagagc ccgccacacg cgatatctgc ggaggagggg gggccatcct cacccgagca
    25381 tgcgacggcg aaaccgccca gccaagcagc caacgccaaa acacccgctc tcgcgcgcat
    25441 gacgagattt ctagcttacc cagccggtcc ggtatagcgc cgcgctgaga cccagcacca
    25501 tgccgcggga gacggagttc tcgggatccc cgtgcccgtt cagcgactag ctgcgagttt
    25561 gcgggtttca atcacttggg cccgcgttcg cgttggatgc gcgttgcctc gcgaatcggc
    25621 acagttactg ggcggcgagc ctcgtacgag ctcggtcgtt cggcccatct ttgggggagg
    25681 ctcgacctcg gatggttcct ccacgattgc ctgcgcgacg tcaggtccga ggtcttgcgc
    25741 gttgtccccc gacggattcg gacgtggccg agcatacagc cccggctctc aggcccgtaa
    25801 agttccgatt agagccgacg cttgacggtc tcatcacggc cgacgaaact cgcgtccctc
    25861 tgttcgaaat gtggccgaca tgcggccgaa cccgcgctcg acacttttgc catggttttg
    25921 gtgcagagcg cgcaagcccc aacaatccga tgccccagac gaatactccg tccgaattcc
    25981 cgcggctgat catcgagcaa gttacgccga ttctggatgc cgggcgctat cccatcaagc
    26041 ggcgtgcggg ctcgcacatc gacgtgggcg cggccatctt caaagatggg cacgacctga
    26101 ttgccgccga tttggtgtat cgcggtccgg gagatagcga agcgcgtcgc gtgccgttga
    26161 actatcgcta cgaggcggat cgctggtacg gcatgttcca cgccgatcgc gcgggccgct
    26221 ggcagtattg gatcgaggct tggccagatg ctttcggcac gtttagctcg gagctgaaga
    26281 agcgcgtcgg agccggtcag gacgttcgct cggagctgct ggaaggcgct gccctgttgc
    26341 tgaaacacgc ggaagggttg tctggcgcga ccaaacttcg ggtgctggac gcggcgcacg
    26401 tcttgaagaa cccggagcgc agcatggagg aacggctcgc cctggcgttc tcggaggagt
    26461 tggtgagttc ggtacggcac ccgttgccgg gtgagctttg cgagtcgccg acgctcgagt
    26521 tgatctcgga tccgcccaag gcctcatttt cagcatggta cgagctgttt ccgcgctccg
    26581 agagcggcgt gccggggcgt cacggaacgt tcgcagatac cgagcggcgt ttgcccgagc
    26641 tcgccaagat gggcttcgag gttctgtatc ttccgcctat ccacccgatc ggccacaccc
    26701 accgaaaagg ccgcaataat gcgcgcgtcg cggagccggg cgacgtgggc agcccgtggg
    26761 cgatcggctc ggaacagggc ggacacgacg cgatccaccc ggacctggga agcatcgagg
    26821 actttcgtcg cctggtgcgc gcggcgggtg agctcggcat cgacattgct ctcgacttcg
    26881 cgctgcagtg ttcgccggat cacccgtggt gcaaggagca tccagagtgg ttcttcatcc
    26941 gcccggacgg cagcatccgc tacgcggaga acccacccaa gaagtacgag gacatctatc
    27001 ccatcaactt ctggtgtgag aaccgcgaag cgctctggaa tgcatgccgc gacgtgctgt
    27061 tctactggat cgagaacggc gtgcgcgtgt tccgcgtgga taatccgcac accaagccgc
    27121 tcgcgttctg ggagtgggtg attcgcgagg tgcatctgcg tttccccgac gtgatcttcc
    27181 tgtccgagtc cttcacgcgc ccgaatcgcc tgaaggcgct cgcgaagctc gggttcaatc
    27241 agagctacac gtacttcacg tggaagaact cctcgtggga gctcaaggag tacatgaacg
    27301 agctgattca gccggagatg ctctcctact tccgtccgaa tttcttcgcg aacacgcccg
    27361 atattctgca cgagtacctg caggtaggcg gcaggcccgc gtttcggatt cggttggtgc
    27421 tcgcggccac gctctcgccg acctacggca tctacagcgg ctacgagctg tgcgaaaacg
    27481 taccggtccg acacggcagc gaggagtact tggactcgga gaagtacgag ctcaagacgc
    27541 gcaactacga cgcgccgaac catatcaaag aagacatact gaagctgaac cggattcgcc
    27601 gcgaaaaccc ggctctgcac gagctcggca atctccgctt ctgcgtgtcg gactacgagg
    27661 gcatcctggc gtaccgcaaa tcggctccca acaacgagct cttgatttgc gtgaacctgg
    27721 acccgttcag cgtgcacgag accatggtgc acgtgccaat cgcggaactc gggattcccg
    27781 aaggcgagcc ctacgaggtt ttcgatctat tgacccacga gcgctacgtt tggcgcggca
    27841 gccgcaacta cgtgcggctc gaccccgccg agcgcgtcgc ccatgtgttt cgcatttctc
    27901 cacgccctct gtgatcgatt gagctgaacg aatcatgcgt gtccgccccg ggaaagccca
    27961 tcctttcgga gcaacgtggg acggcgaagg cgtcaacttc gccatctttt ccgcggacgc
    28021 cacggccgtc gagctgtgtc tgttcgactc cgtggatgcg aagaaagagt cctttcagat
    28081 caagttgaag gagcgcacca accgcgtctg gcacgtgtat ttgccagatc tgcggcctgg
    28141 ccagctctac gggtaccgtg tgcacggccc gtacgcaccg ctccaaggcc atcgcttcaa
    28201 ccccaacaag ctcctcctgg acccgtacgc caaggccatc gcgggcggct cgcgcttggc
    28261 ggacgaacac ttcgggtatc gaatcggaga tccggccatg gatctctcgt tcagtgagac
    28321 cgatagcgcc cccgtgatgc ccaagagcgt ggtcgtggaa ggggcgttca gctggggcga
    28381 cgataggccc ccgctcgtgc cctggagccg cacgctgatc tacgagtgcc acgtcaaagc
    28441 catgacgatg cgccacccgg agatccctgg gcatttgcgc ggcacctatc tcggtctctg
    28501 ctcggagccg atcatcgatc acttgaagtc tctgtccgtg acggccgtgc agctcctgcc
    28561 cgtgcaacac gcaattagtg agcggcgcct cacggacatg aacatgatca actactgggg
    28621 ctacaacacg atcggttttt ttgccccaga tccgcgcttc gcgaccggtg cgttgggcca
    28681 gcaggtcacc gaattcaaga ccatggtcaa ggtgctgcac cgcgccggga tcgaggtcat
    28741 tctcgacgtg gtctacaacc acaccgcgga aggtaacgag ctcggaccga cgctgtgttt
    28801 tcgcggcgtc gaccagtgcg cctactacaa gctgtccccc aacaacccgc gccatcacat
    28861 cgactacacg ggctgcggta acagcttcaa tctatcgaac ccgcgcgcgc tacagctggt
    28921 tttggacagc ttgcgttact gggtacagga gatgcatgtc gatggcttca ggttcgatct
    28981 cgcggtcacg ctggcccggg atccgtacga gttcgacatg ttttctcgct tcttcgccac
    29041 agtgcaacaa gaccctgtgc tgtcgcacgt gaagctcatc gccgagccct gggatattgg
    29101 gcccggcggc tatcgcgtcg gcggcttccc gcccggctgg gcggagtgga acgggagata
    29161 ccgcgattgc gtgcgtcgct tctggcgagg tgacgaaggt caggtaccgg agctggcctc
    29221 gcgtttgtcg ggttcgagtg acttgtttca tggcggagat cggggcccct ttgcgagtgt
    29281 gaatttcgtc acctgtcacg acggttttac cctgcatgac ttggtgagtt acgagcacaa
    29341 gcacaacgaa gccaacgccg aagaaaaccg cgacggaacg aacgacaatc agtcgcgcaa
    29401 ttggggcgtg gaaggtgaaa cccgtgcgca gcaagtgcag cgcttgcggg atcgcatgaa
    29461 gcgcaacatg ctcgcgctgc tcgcgttctc tcaaggggtg cccatgatct ctcatggtga
    29521 cgagattggg cgcactcagc gcggcaacaa caacgcctat tgccaggaca acgatctgac
    29581 ctggttgcag tgggatttgg acgcgaggca gcgcgagctg ctcgcattca cgcgggaagt
    29641 tttccgcatc acgaacagta acccggtatt ccgtcgccgc cgttttttcg atggcgatcc
    29701 ggtgagcacc tctggcgtca aggacgtctc ttgggttcgg cccgagggcg gcgaaatgac
    29761 gaatgaggac tggggtaatc ccaaaaatca ttcactgggc atgctcatcc acggtgaagc
    29821 gtccgacgac gtggatgagc gcggtcgccc caatcgaggc caaacgctgc tgctgctgtt
    29881 gaacgcgagt gtgagagcgc gcagcttcgt gctgcccgtt ttgccggagg ccggtcactg
    29941 gcaggaggtg gtgaataccg cccagtcgac caagcggatc ccgaaggggc ccgcgattag
    30001 cgttgctcca cactccctag tgctgctttg ctacgtgcct ggctctgctg acaaggtgtg
    30061 agaagaggcc gacaggttcg agagagccga aacggttaga ggaagaggtt gtctacgctc
    30121 atggattcgc tgaacgtcga tcccgtcctg gctggtgatc ctgaaaagct cgaagccctg
    30181 cggcgcgatc gtttgcacga tccgcacagc gtgctcggag cgcaccccac gcgcgtcggg
    30241 tactccgagg gcatcgtggt gcgctcgtac cacccggagg cggagggctg tgcgctgctg
    30301 ctcggcagcg aggtgaggcc gatgacgccc ctgggcggcg gcatcttcgc gacctttttg
    30361 gaaggtgcgc ggctgcccat ctcctatcgg ctgcgcttct ctttcgaggg cggggcggtg
    30421 tgggagcgtg atgacccgta tcggttcctg cccacgctcg gtgagttgga tcaacacttg
    30481 ttcagcgaag gcacccaccg ccggttgtgg gaggtgctcg gtgcgcgcgt gcgcgtgcaa
    30541 gagggcacgt ccggcaccgc gttcgcggtg tgggctccgg gcgcgcgtcg cgtgagcttg
    30601 gtcggagact tcaatcgctg ggacggccgt ttgttcccca tgcgttcgct cggcagcgcg
    30661 ggcgtttggg agatcttcgt gccggggctc ggcgcgggtg cgctctacaa gttcgagatc
    30721 aagacgcaag acggcgcgct gcgcatcaag accgatccga tggcgcgcga gatggaacac
    30781 cctcccgcga ccgcttcgcg tgtcaatgaa gcgcgccaca tctggggcga tgacgcttgg
    30841 atgcaggcgc gtgcggggcg tgacattcgg cgcgagccgg tcaccatcta cgagctccac
    30901 ttgggttcat gggcgcgcgt tccggaagaa ggcaatcgct tcctgagcta ccgagagatc
    30961 gcgcaccgct tggtggcgca cgttcagaag ctcgggttca cccacgtgga gttgatgccc
    31021 gtcgcggagc acgcattcta cccgtcttgg ggctaccaag tgacgggcta ctacgctccg
    31081 acctcgcgct acggcacgcc cgacgatttc aggtacttcg tggactactg ccaccagcac
    31141 ggcatcggcg tgatcatcga ctgggttccg gcgcacttcc ccaaggacga ctttgcgctg
    31201 aggcgtttcg acggcaccgg cttgtacgaa cacgaagaca tgcgaatggg cgagcacccc
    31261 gactggggta cgctcatctt caattatggc cgtcgcgagg tgcgaaattt cttggttgcc
    31321 aacgccctct attggctgcg tgagttccac gtcgatggcc tgcgcgtgga tgcggtggcc
    31381 tccatgctgt acctcgacta cagccggaaa gagggcgagt ggctcccgaa cccgttcggc
    31441 ggacgggaga acctggacgc catcgacttc ttgcgcgctt tcaactcggc gatcaccgaa
    31501 gaggttcccg gcgcgttcac gatcgccgag gaatcgacgg cttggggtgg catcacgcgg
    31561 ccgcccgccg agggcggctt gggcttcacg ttcaagtgga acatgggctg gatgcacgac
    31621 accctggcgt tcttcacgaa ggagccggtg caccgcaagt atcacgtgga tcagctcacg
    31681 ttcgcgatgc tgtacgagca caccgagtct ttcatcaact cgatctccca cgacgaggtg
    31741 gtgcacggca aaggcgcgct ggtcgagaag atgcccgggg atttctggca gaagctcgcc
    31801 aacctgcgct tgctcctgac gtaccagtac acgcgacctg gcaagcaact ggtgttcatg
    31861 ggcaccgagt tcggccagca ccacgagtgg aaccacgact cttcgattga ttggcacatc
    31921 gcggagcacc cccagcgccg agccctgcag gacttcatca ccgagattgg tcgcttgtat
    31981 cgcgcgatgc ccgcgttgtg gcgttccgat ccgatgccgg agagcttcga gtggatcgat
    32041 tgcagcgaca aagacaacac ggtgctcagc tatctgcgtc gcgacggcac ggacttggtc
    32101 atcgtagtgt tgaacttcac gccggtgccg cgggaagggt atcgagtcgg agtgccgatc
    32161 cctggtcgtt acgtggagcg gctctcgagc gacgacttgc ggttcggggg cagtgagttc
    32221 gagaccttgg cagtcgtgga gacggatcct atccccgcgc acaaccgcaa ttactcgttg
    32281 aagcttcagg tgcctccgct cggggcgctg atcctgtcgc cggctaagtg acagtccgaa
    32341 gtgacaagtc cgaactgaca tagtgcggcg ccgcccgctc tgcgggcggc gcgagccgtc
    32401 gacgtgacga ggtggcccga gcgcggtatc gcgccgggcc gcgtgctgtt gtgcctgcgc
    32461 gagcggttag ctcagacgag tgtcgcgccc gtagtagcgc cggctcgaag caaacaccaa
    32521 tccgtggtta cggcaccgtc gtgccgcagg cgcattcgaa gtagctgacc gcgagctgcg
    32581 cggggccgtt cggggtgagt gatgagccgc ccgattgatc ccaggcattc gtgcacttgt
    32641 tgccctgtcc ggtcatcgcg ttgcgcttca cacacatggg atcgccgccg gtgccgcagc
    32701 cagggttcat ctgaacgcag gtgatgatgg cggagcactg cttcgcgcag gttcctccgt
    32761 cgttggtcgg gcacgcttgc gtgcagttca tgcagctggc gccggtccag tcgcaggctc
    32821 ccgagctgct tcccgcggag ccggggctgc ccgcggagct acggcccgcg gtcgttgcgg
    32881 ccccggcgct ggcgctgcgg cctccagatc cgctccgccc accggacccg gcggagccgc
    32941 gccgtccgcc cgtcgtgcgt ccgccagtgc tggcgcgggt gccgccctga tcaccagtcg
    33001 ttccgccgtt gctcgtggcg ccacccgatg ccgtggtctc ggtggcggta gtgccgcctg
    33061 cgccgacgct gttgccgcca ctgcccgcgg gcggtgacgg gccgccatcg ccgtactcac
    33121 caatgctcac cgggtcttcg acggtggcgc acgcgccgac gctggacgcg agcagcgcgc
    33181 tcaccccgag ggaagagagc acgatcgagg agcgcgcgcg gaagcgcgag cgatacggac
    33241 gccggggact cgagcgcaag ccaagcagca tcacttcgca cggctaacgc agccacattc
    33301 gccccgcaag tcacctcatc acgagcacga cacgaatcag ctcgccgggg gctcggcgta
    33361 ttccagtgcc tgttcagcgt aggtagttgg cacggcgtcg agcttgcaac gcgattgcac
    33421 ttttcggctt cgggcaagct cgacggttcg cacgccgctg cggcacagca cgctaaaacg
    33481 cggcgcggct acttcacgtc tacgtagaaa tcttcgctct ttaggttcgt gatcgggcca
    33541 ttccagatct caaagcccac ggccacgctc agcaccgtgc ttccgggggc atcgatagag
    33601 cgcgtcttca cgtcgcggat gaagtccatc acgtcgaatt cgagctctgg cagatcttgg
    33661 ccctcagggc gcgcgtagct gatgcagggc ttgcctccga ccacgccaac ccaaacgtcc
    33721 caagtgccct gcacgttggc gacctgcacg gtgttcaact ttttgctgcc ggcgggttgc
    33781 tggccttggg ggtcgcgcag ccagaccatg aggaacgagc ggtgggtact gacggtattg
    33841 ccatcgccga gccagatatc gtacgcggcg ttgtattggt tggtgttgcc gttggcgctc
    33901 cagcgccaac cggtgcgcaa gctcttgatg tccgcgatgg ccttgggcag gccacacgac
    33961 tgcgattggt catccgagta cacgccgcag aaggtggtcg ggtacgaagc aggctcccag
    34021 ttgccgccct gcttgccttc catgctgacg acggtgaatg aggtgccgct ccagctgacg
    34081 gattgagatt ggaagcctgg cccccagccg ttcgccatga agcgataatt cacgccgttt
    34141 cggatcacgt cggccttggc gtatcgctcg ggggtcgagc cgaggccctg ttgcatgggc
    34201 gtgccgaagc ccgaaggccc ggtgctcgtg ctgccgccag tggcgttagt gctggtgctc
    34261 gtggcgccgc ctttggcggc cgtggtgccg ccggcgccag tggccccgcc gctgctgctc
    34321 ggggttcctc ccgcctgcac ggggggactg ccagcactgc cttgagtgtc agtgctcgaa
    34381 ccgaagtcac aggcaaccaa gggaagagac aacaacaggg ctgagatcga aggcgcaagc
    34441 ttagtcatga acggacctcg aactttggca gcaagaagga cacgagcacc aaggagccca
    34501 aagttgggct ccttgggcgt tggcaaaggc ttggaaggca ggtcttggca gattagtagc
    34561 aggatgccag ctcgccaggc ccagcggccg taaagttgcg caaaaacgag aggtcgctcg
    34621 cgctcttctg cagcttttga cgccccgagt gggttgtcac cactggtatg ctccgccgcc
    34681 atgccgctct accttggtct cgactccagt acacagagct tgactgcgct cgtcatcgat
    34741 acggacaccc agcaagtcgt gctcacacgt tcggtcaatt tcggtgcgga cctgcccgag
    34801 tatgcgtcga agcacggctt cctcgagaat cccgacccga aggtgcgccg ctcgaacccg
    34861 ctgatgtggg tcgccgcgct cgagaaattg ctgtcggatg tgcgtgcgag cggcttcgac
    34921 tgggggacca tcgcgggcat cagcggcgcc gggcagcagc acggctcggt gtacctgaac
    34981 aagcccatct ccgaggtggg cggctggtcg acgagcgcaa cgttggtgga tcaaatccgc
    35041 ccgctgctga gccgcagcga ggcgccgatt tggatggata gctcgacgag ccaagagtgt
    35101 cgtgagctag ccgaagccgt gggtggcccc gagcgcgtga cgcaaatcac gggctcgcgc
    35161 ccgatcgagc gcttcacggc ggcccagatc cgcgcgttct acaagcagga cccggaagct
    35221 tacgcgcgca cgcgtgaaat ccatttggtc agcagcttca tggcttcgat tttggcgggc
    35281 cgctcggcgc cgatcgactt cggcgacggc gcaggcatga acttgctcga tctagaccag
    35341 ctgaagtgga acccggagat gctcgaggcg accgcgccgg agctcgcgcg gcgcttgttg
    35401 cccgcggtcg cgtcgtccac ccaagtcggg accgttgccc agttcttcgt cgagacgtac
    35461 ggcttcaaga gcggcgtccc ggtctatgcc ttcacgggtg acaatcccag cagcttggtc
    35521 ggcacgggtg cggtgcgtcc gggaaccgcg atcgtgagtc tgggaacgag cgacacggtg
    35581 ttcgccgcca tgcgcaaccc gcgcgtggat ccgcgcgggt acgggcacgt tttcggcaac
    35641 cctgcgggcg gcttcatgtg cttggtgtgc ttcgccaacg gctcgcttgc gcgcgagaac
    35701 gtggcgcagc gcgtggggct cgattggccc gcgttcgaga aagcgatttt ggatacgccg
    35761 cccgggaact cgaacgcatt gttgctgccg tacttcgtgc cggagatcac tccgaagatc
    35821 ctcaagccgg aggcgcgggc ctttggtagc cccgagttcg tggagttccg tgcgcgtgag
    35881 cagatggctc gcgcggtggt cgaagcacag gcgctcgcga tgcagcgcta ctcggattgg
    35941 atcggtgaga cacccgatct gattttggcc accggcggcg cctcggccaa tcgcggcatt
    36001 cttcaaatcc tagccgacgt gtttggcgcc gaggtgcgta cgctggaggt caacaactct
    36061 tccgcgctcg gcggcgcgtt gcgcgcggcg caagcccaca gcggcctggc gtgggaaaag
    36121 ctattcgaag ccttcgtgag cggcggccgg cagctgcacg tcaagccgaa caccgcggcg
    36181 aattacacgg ctctgagtca gcgcttcgcg gaagaagtgg accggatcac cacggtttga
    36241 agcgccgatt cgtgccgcgc gctcccaacc gagctcgcac gcttcgcctg gtgttggctg
    36301 gtggcgcgat cgctagccaa ctcaccgtga gctcggcggg tcgggcgcag gcacgcgtcg
    36361 ggtccgaagt tcatcggacc tccctctcgt cctcgagccg ggcgccgtgt cgcgtggacc
    36421 gcggacgacg cacgttggca atcggggccg cggtggtgcc cggcgcggtg gtgcatggtg
    36481 cggggcactt cgtcgcgtgc gagcgggaga ccgcaaagaa gctcgccttg gcggaggccg
    36541 ccggcgtggg cgcactgctc acgtcgggag tgctgttgtt gctcaccggc gcatctcagt
    36601 acgtggttgc gccgctcgcc gtcggcgcgg tgaccggcgg atcggtattc ggcctgagtt
    36661 atttggccga tgtgtacggc gtcgttgctg acaatgacca agccgcaccg cgcgcggcgc
    36721 gcctgcgggt ggaggcaggg ttcagctacg tctacgatcc gcacttcagt taccgcacgt
    36781 tcatcagcca aggctttcgt ctccaagagg ggcggtattg gctcgcgccg cagatcatgt
    36841 cgtcgctcga cgcgcacaat cgacgttacg gcgcgcgcgc tggataccga gttctcggcg
    36901 gcgcgcggcc tacgcaggtg gcgtcgatat cacggctcgc gccccggtcc tggctggacg
    36961 ttgcgctcgg cgcgtatgac catggttatg gtgaatccgg cttctcgcta accggggtcg
    37021 aggcggccct gctcggtcac ctcgagctct cggcgctcgg tgctcgcact ctgcgcggcg
    37081 ccttcgccga aggcgagctg ggttacgccc gcttgtggac tcgcttcgat ggcctgccgg
    37141 atctacccaa cgatcagctc ctggcccgct tcatgttcgg attctactgg gggcgcgggc
    37201 aagccttgga gggccagacc cagtttggct atgaccaccg acgcgatacg ctcgcggggg
    37261 gattgaagcc ggcggggatt gcggcgggct a
//
LOCUS       DASTKU010000533        11316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_772303, whole genome shotgun sequence.
ACCESSION   DASTKU010000533 DASTKU010000000
VERSION     DASTKU010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11316
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772303"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1105)
                     /locus_tag="VFQ61_18520"
     CDS             complement(104..1105)
                     /locus_tag="VFQ61_18520"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9956510.1"
                     /translation="MTMRSRQVWFVVFPGSEMLDISGPWAVLSHANDFLRSPAYELHL
                     ISPKGGVVSTGHGVQLAGARSLVSMTRRGTPDLVVISGGSPLEPMPAPELAMARWLTR
                     HQSAIPRLVSICTGAFVLAEAGLLKGKRATTHHLWANELGRRYPDVLVSAREIFQRHG
                     RVWTSAGITAGIDLALALVEQDHGHAIASAVAKKLVLFLRRSGNQTQFSRALEYQEKE
                     PTELRGLVAFVLEHLSETLSVPRLARKFGMSARSLTRQCHSKLGASPAALVRGLRIDR
                     AKRLLEETNLPLKSIAADTGLGDASTIWRYFSQQLGVTPAEYRARFASDSKPAYEQR"
     gene            1236..1886
                     /locus_tag="VFQ61_18525"
     CDS             1236..1886
                     /locus_tag="VFQ61_18525"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9956511.1"
                     /translation="MLEFAGIRLPDSPLCLAARELVARIGPPALEHHVLRSYLYAEWI
                     GQKRRLTYDREVLCVAAVLHDLGLTRLAPVRSRFELEGADLAREFLSTRGLDAARSDL
                     VWEAIALHTTAEIAQRRSVEVGLCQLGVAADVRGLPEVEAEPQLVDQVLEAYPWLDMD
                     QALLALLVGLYQKNPASSASHAVADACERFVPGFTRVNLCDVLLERARRSRTHSRS"
     gene            2393..3196
                     /locus_tag="VFQ61_18530"
     CDS             2393..3196
                     /locus_tag="VFQ61_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956512.1"
                     /translation="MKRSRFFELGLVAFAATVCACCEPKLVVGEWNCPGRYGSAGESG
                     ESEEVPIPFEPVAIPWSTSFETGFCDYQRIQGFCYVSSGGSYSIVSSPVHSGKFAAAF
                     KANGRGAQSRCITQGEFPKQAYYSAWYLVPAFASNTGNWNLLHYQGGMPPDNLDVLWD
                     VSVSSNADGDLNLYVFRRLSRDRMPQVDAFPRIPIGVWFHIEVFVRRASDPTGELAVY
                     QDGQLVYRATDIVTDDTAWGQWYVGNLTTSLMPPDSTVYVDDVTFSETR"
     gene            3288..4292
                     /locus_tag="VFQ61_18535"
     CDS             3288..4292
                     /locus_tag="VFQ61_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956513.1"
                     /translation="MSWDAPAGCPTGEVVQERIRELLGSAAAKATHLQAQGKIVRAKG
                     RYRLTLTTLQDGGGASERTIESSSCSDLAGAAAVTLGLWIRFELAAEQRASESAQPRE
                     SEGAQAGASGERAAPNDSAAAATADKPASESRATPEPQTPDVSKENAERSHADDSVSD
                     ASKLHLLVHAPLIGVEAGPLPGLRPSAGLALGFRLRRWRALLGARATLGETIWASGNP
                     RRGAELQRLTAELRGCYGFGEDRFEFSPCLFLAVERVAAKGVGGDEIVTSSGRTFLLA
                     PGLGVLGNLHLTSSLGLFVSLGAQIETSRPRLVVEGLGQIRQLAQLGLAAHMGLEWIL
                     "
     gene            4350..4931
                     /locus_tag="VFQ61_18540"
     CDS             4350..4931
                     /locus_tag="VFQ61_18540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9956514.1"
                     /translation="MALAEANPDGPLTPPGAVPSFAEVYERFFDFVWASTRRLGVGTE
                     WIDDVVQEIFIVIHQRLHSLERPDSLRSWVYGITRRTVSTYHRARRSKGAGTDTLSLN
                     ADTLETQSPNPLELTEQNDQVKLLESLLAELAPPKREVFVLAEIEELSVPEIAEALEI
                     PLNTAYSRLRAARQAFEAALARHTARSVRGKTS"
     gene            4928..5626
                     /locus_tag="VFQ61_18545"
     CDS             4928..5626
                     /locus_tag="VFQ61_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956515.1"
                     /translation="MNELTPIAKELVRAGRAASRPNPADRERISHALQARIDISGAPN
                     APTPPTQSLSLLGKGWLGWTGIGIAALCGLGAVVLIVMSSSAPVAMVMPRIEQSVPAQ
                     LAPTPADAVVVSSAEPAEEVTKNEAPSRSPSPMASARSSDRLADEVAILSRAEADLHR
                     GQASSALRSLDEHQRKFPSGALSLERRAARVRALCALGRSNEATAELKRLTRSAPGSP
                     LERRAREACGTRVN"
     gene            complement(5698..6774)
                     /locus_tag="VFQ61_18550"
     CDS             complement(5698..6774)
                     /locus_tag="VFQ61_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956516.1"
                     /translation="MVLNRTSSHVLVLAAVHSIVFACSTESSDQPGTKSFASGGFGSG
                     VASTSISTAGTGGVQPVGGAYSSSGAASSGGVSASHGTNPSSGGGATSPAGGSSNAMS
                     GGAPAAGAAQGGAQPTGGVTASGGTTDAAAGMTAGGAVSGGAPNSEGGAMSSGGSSPG
                     GAGQTAGEPASAVRGIDGLRIDDPCSGTPSITDGATCNHTTLTNNRFRASKEVAVGGD
                     ANEVYDVTLRIRGVVEPTNIDGGSRPDTSTFVYKSEMWRKVPYTIGGTVKQADYEQWS
                     ITVSAPPQTYFLNDYQKGAHNIFKLDYPITIPIAAGAKVTLECSDSNEREIVNYEKYT
                     LEGLPGSLNFGQFVQVNVVAAKAR"
     gene            complement(6877..8310)
                     /locus_tag="VFQ61_18555"
     CDS             complement(6877..8310)
                     /locus_tag="VFQ61_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9956517.1"
                     /translation="MNARSPIRPVINRRAFLRGAGGVALALPFLEGLPERSAWAAGSA
                     PVFTFFICAACGVEPKRFWPSGSGALSGLLTSGKAVDALAAHSANLLIVKNVNFPQTG
                     PTGCGHAQGLSQALTAKTPTGNGGAATATGPSADWVISKAVNPSGTEPLTLYAGNVKN
                     GYIADRLSFDDKGQVRASVDNPYKLYQRLTGLASPAGGGTTGGTGTSSGANPMAQELV
                     KKRKSVNDLVRRELNSLLQNPKLSAADKQRLQQHFDSIRDVEGTMMTMGTQMAAVGCA
                     MDGVDTSVYDALKSWKYNPTNAANGGIENIVELHLQMVALAFACNLNRTGTLQWGDGT
                     DHTVYQVPSNQTLGNWNLHYISHRTQSDGNIGQNSTAEAAHAEIDQLRMQTLARGLEH
                     FKARGLQDKSIVLWTNHVAEGNHSMRNVPFILWGSGGGAIKQGQLVDGGGQNNGTLLN
                     VVIGAATGTPTTNFGASGGKTLSAIQA"
     gene            complement(8307..10052)
                     /locus_tag="VFQ61_18560"
     CDS             complement(8307..10052)
                     /locus_tag="VFQ61_18560"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9956518.1"
                     /translation="MHKPRLRALAQSFVLVLGCGCTGSISEVGGSAGTPGMTPSGAGN
                     SPGGPTSGSGGQAGNSPAGAASNSGGAGTSTPPQPGQPRPVSMDGTPIYSRFLRLTND
                     QWENSVRDVLKLSAPTGVSSGFLHAVSGTTDFDNNERVLVVNNTIWGDFQLAAETVAA
                     QVTATDAALQTVVATKDAATFVKTFGRRAFRRDLTSEELTSYQALLAEGATYSGSQSA
                     FTKGAALVIAAMLQSPHFLYRIELGDTGKLLSGYELAAKLSLWIRDTTPTESMLDAAK
                     SGAFDSPDGAAAQAKSMLDDAASTTVMRKFHGQLYKIEVLDTIVKTGVQGYTEALLPE
                     LKEASYRFFDQIFTQNLGLKEILTTSVGFAGPKMAALYGVSTQGSGVQKVTLQNRAGW
                     YSQLPFLTLWAINNDPDSVHRGVRINLDTLCADPGLPTVNLPPVPPLQANQTNRQRYQ
                     ALTEGCGATCHGEIINPLGFAFENYDGVGRFREMDNGQNVDAQGRYPFAEGTKSFSGA
                     PELMQIIASGTQAHQCYAKKLASYALGRDLIDTERPLIEALGATSLATGASLKEVMIA
                     LVKTDAFRTHVGGAQ"
     gene            10448..>11316
                     /locus_tag="VFQ61_18565"
     CDS             10448..>11316
                     /locus_tag="VFQ61_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956519.1"
                     /translation="MNSALWAWLGGIFAIVGCRPDASTSPIPGTVQAGGATALPSFGG
                     SAGATNMQSGGITSGGATGATSSGGSGLVAGTGSMPASGGTASTGGASGGVNTGGQGT
                     GNAAGMGGATTAGMNAGGATNTAGSSAGNTGGASGGAAGAASGGGSSKAVPSAGCGKS
                     GRPNGGVVTVNNDHIYTFPTSYDGTKPLPLLIGFHAAGNPIDQIQKLTNGSSFESNYV
                     RAFPKSAGNEWVYNTDLNKAYKVYDDLLANYCVDTSRVFATGHSSGAQMIVQILVHKD
                     AADHFKFKAVAPVA"
BASE COUNT         2035 a         3455 c         3569 g         2257 t
ORIGIN      
        1 cagagtcaca gtgcacgcca gagtcacagt gcacgccaga gtcacagtgc acagccagtc
       61 cagtccagtc cagtgcggtg gccggtcgct ggctgacgtg gttctatctt tgctcgtatg
      121 cgggcttcga gtccgaggcg aagcgcgcgc ggtattcagc cggggtcacg cccagctgct
      181 gactgaagta gcgccagatc gtgctggcgt cgccgaggcc agtatccgcc gcgatactct
      241 tgagagggag attggtttcc tcgagcagtc gcttggcgcg atcgattcgg agtccgcgaa
      301 ccagcgccgc tggcgacgca cccagcttcg agtgacattg gcgtgtcaat gagcgagcgc
      361 tcatcccgaa cttgcgcgcg aggcgtggca ccgagagcgt ttcactcaag tgctccagta
      421 cgaacgcgac caagccgcgc agttcggtgg gttccttctc ttgatattcg agcgcgcggc
      481 tgaactgagt ctgattgccg gagcgccgca aaaacaggac gagcttcttg gcgaccgcgg
      541 aagcaatcgc gtgcccgtga tcctgttcga cgagggccag cgcgagatcg atgccggcag
      601 tgataccggc tgacgtccaa acccggccgt ggcgctggaa gatctcccgg gcgctcacca
      661 gtacgtcggg gtagcgccgc ccgagctcgt ttgcccaaag gtggtgcgtc gtggcccgct
      721 tgcctttgag taaacctgct tccgccagca cgaacgcgcc ggtgcagatc gagacgagcc
      781 gcgggatggc gctctggtgg cgagtcaacc aacgcgccat cgcgagttct ggagccggca
      841 tcggttcgag cggtgaccca ccggagatga caaccagatc tggagttccg cgtcgcgtca
      901 tgctgaccag cgagcgggcc cctgccagtt gcacgccatg gccggtactc acgacgccgc
      961 ccttcggcga aatgaggtgc agctcgtacg cggggctgcg aaggaagtcg ttggcgtggc
     1021 tgagaaccgc ccacggtccg ctgatatcca acatttccga gccgggaaac accacgaacc
     1081 aaacctgacg ggagcgcatg gtcatgtaag ttgattcgga tgtcctagaa tgcaaggaac
     1141 ttgtcattta ggacatacgg cattctggct aatctccagg gcgtcagtaa gcagtgcgcc
     1201 tctccgatcg agcggcgccg ggaagaaggt ctctcatgct cgaatttgcc gggattcgtt
     1261 tgcccgatag cccgctgtgc ctcgccgccc gagagttggt tgctcgaata gggccacctg
     1321 cgctcgagca ccatgtcttg cgctcctacc tgtatgccga gtggatcgga cagaagcgtc
     1381 ggctcaccta tgatcgagaa gtgctgtgtg tggccgctgt ccttcacgat ctcgggctca
     1441 ctcggctggc gccggttcga tcacggttcg agctcgaagg cgcagacttg gcgcgcgagt
     1501 ttctatcgac acgaggtttg gacgcggccc gctcggacct ggtttgggag gccatcgcgc
     1561 tgcatacgac agccgagatc gcgcagagac gttcggtgga ggttggcctg tgccagctcg
     1621 gagtcgcggc cgacgttcgt ggtctgccgg aagtcgaagc ggagccacag ctggtggacc
     1681 aggtgctaga agcgtacccc tggctcgaca tggatcaggc gctgctcgct ctactggttg
     1741 ggctgtatca gaagaacccc gccagcagcg cgtcccatgc cgtggcggac gcctgcgaac
     1801 gcttcgtccc aggattcacc agggtcaact tgtgcgacgt gttactcgag cgcgcgcggc
     1861 gctcgaggac gcattcccgc agctagtcgt tgccatcgac atcgagtcat cgctagcgca
     1921 ccaaaggttc gaagccgcgc cgcgtgcgcc gatccccagt ttcatagccg caatcccgcg
     1981 tacctgcagg gtgacctccg atcggaagct cgccgcgacg cagtccgcgt gccggcgaga
     2041 gccgaacgag cgacgcagct gacccctccg gccgacgggt tcgaagccgc tgaatgctac
     2101 tgccgcggtg gcgtgaacgg ctaatttcgg cctgactgag tgcgcacggt ccccgattgc
     2161 tttctctgac ggaatggatg gcaccctgcc actgcgaatc cggataggtg tacgcctcgc
     2221 gtggttgtct ctcggcgtac gcctccagat gtgtggctaa tcccagggat cgcgagcggt
     2281 gccggcggcg tgtgcgcacg gggtcgcgtc tccggctaac gcgcacgcac aatcgatttc
     2341 ggctcaattt cttggatgac aatttcgccc gaaccctcaa cactcggggc gagtgaagcg
     2401 ttcgcggttc ttcgagctgg gtctggttgc cttcgcagcg acagtgtgcg cgtgctgcga
     2461 gccgaagttg gtggtcggtg aatggaactg cccgggtcgc tacgggagcg caggtgaaag
     2521 cggcgagagt gaagaagttc caattccgtt cgagcccgtg gccattccgt ggtcgacgag
     2581 cttcgagacc ggcttttgcg attaccagcg gatccagggg ttctgctacg tgtcctccgg
     2641 cggctcgtac tcgatcgtct cctcaccggt ccactccggt aagttcgccg ccgccttcaa
     2701 ggccaacggt cggggcgcgc agagccgctg cataacccag ggggagttcc ccaagcaggc
     2761 ttactactcc gcctggtatc tcgtgcccgc cttcgcctcg aatactggga attggaattt
     2821 gctgcattac caggggggca tgccgccgga caatctagac gtgctctggg atgtgtcggt
     2881 ctcgagcaac gcggacggag acctcaacct gtatgttttc agaaggctgt cccgagatcg
     2941 catgcctcag gtcgacgcat ttcctcgcat tccaatcggt gtctggtttc atatcgaggt
     3001 gttcgttcga cgcgcttcag acccaacggg tgagttggcc gtgtaccagg acgggcagct
     3061 cgtgtaccga gccacggaca tcgtgaccga cgacacggct tgggggcagt ggtacgtcgg
     3121 aaatctcacg acctcgctca tgccgccgga ctccacggtt tacgtagacg acgtgacgtt
     3181 cagtgaaact cgttaggtgt tgtcggaaaa cgaggtggct gctgcccgct gctagcctct
     3241 ttctctaccc gcattctgcg caggccgagc cacggcaggg cctcgcattg tcttgggacg
     3301 cgcccgccgg ctgtcctacg ggtgaggtgg ttcaggagcg aattcgcgag ctgctgggct
     3361 cagccgctgc gaaggcgacg cacctgcaag cccaagggaa gattgtgcgc gccaagggcc
     3421 gctatcgtct cactttgacg actctgcagg atggtggcgg cgcgagcgaa cgcaccatcg
     3481 aatccagctc ctgttctgac ctcgcgggcg cggccgcggt cacgctcggc ctatggattc
     3541 gattcgaact ggccgccgag caacgagcat ccgaatcggc tcagcctcgc gaatcagagg
     3601 gtgcgcaagc cggggcgtct ggtgagcgcg cggctcccaa cgatagcgcc gccgccgcaa
     3661 ccgccgacaa gccggcctcg gagtcacgcg ccacgccgga gccacaaaca cccgacgtga
     3721 gtaaagagaa cgccgagcgt tctcacgccg acgattcggt gtcagatgca tcaaagttgc
     3781 atctgttggt gcacgccccg ctgatcggcg tggaggccgg gcctctcccc ggtttgcgtc
     3841 cgtcggcggg cctcgcgctc ggatttcgcc tacggcgctg gcgggcgttg ttgggggcac
     3901 gagcgactct tggtgagacg atttgggcga gcggaaatcc tcgccggggc gcggaactgc
     3961 agcggctcac tgccgagctt cgaggatgtt acggctttgg tgaggatcgt ttcgaattca
     4021 gcccctgcct tttcctggcc gtcgaaagag tcgcggcgaa aggcgtaggg ggggacgaga
     4081 tcgtcaccag tagcggacgg acatttctgc tcgcgccggg cctgggggtg ctcgggaacc
     4141 tccacctcac ttcgtccctc ggcctattcg tctcccttgg agcccagatc gagacatcgc
     4201 gcccgcgctt ggtggtggag ggtctcggac aaatccgaca gttagctcag ttggggctgg
     4261 cggcgcacat ggggctcgag tggattttgt gacggaaagt gaacgccgcc gcgactgcat
     4321 cacaatagat gatggctcgt gagccgaacg tggctttagc cgaagctaac ccggacgggc
     4381 cgttgacacc cccgggcgcg gtgccctcgt ttgcagaggt ctatgagcgg tttttcgact
     4441 tcgtttgggc ttcgacccgg cggctggggg tcggaaccga gtggatcgat gacgtcgtac
     4501 aggaaatctt catcgtcatc catcagcggc ttcactcgct ggagaggcca gattcgcttc
     4561 ggagctgggt ctacgggatc acgcgccgca cggtaagtac gtaccatcgc gcacggcgaa
     4621 gcaagggtgc gggtacagac actttgagtc tgaatgcgga cacgctggag actcaatcac
     4681 cgaacccgct cgagctcacc gagcagaatg accaagtgaa actcctcgaa tccttgcttg
     4741 ccgaactcgc tccgccgaaa cgcgaagtgt tcgtactggc cgagatcgag gagctgagcg
     4801 tacccgaaat tgcggaagcg ctcgagattc cgctgaatac agcttactca aggttgcgcg
     4861 ctgctcgtca ggcgttcgaa gcggccctgg cgcggcatac cgcacgatca gttagaggga
     4921 agacgtcgtg aacgaactca cgccgatagc caaagagctc gtccgcgccg gtcgcgcggc
     4981 gtctcgtccg aaccccgcag atcgcgagcg catctcgcac gctttgcagg cgcgaatcga
     5041 catttccgga gcgcccaatg ctccaacgcc gccgactcaa tcgctctctt tgctaggcaa
     5101 aggctggctt gggtggaccg gcatcggaat cgccgctctc tgcgggttgg gagcggtcgt
     5161 gctcatcgtg atgagcagta gcgctccggt ggcgatggtg atgcctcgta tcgaacaatc
     5221 ggttcccgcg cagctggctc ccactccggc ggacgcagtc gtggtgagct ctgccgaacc
     5281 agccgaagaa gtgacgaaga acgaagcgcc atcgcgcagc ccatcgccga tggcgagcgc
     5341 gcgatcctcg gatcgcttgg cggacgaagt ggcgattcta tcgcgtgcag aggccgatct
     5401 gcatcgtggg caggcgagct ctgcgttacg cagcctcgac gaacaccagc gcaagtttcc
     5461 gagcggtgct ctgagcctcg agcgtcgtgc cgcccgggtg cgcgcgcttt gcgcgctggg
     5521 gcgcagcaac gaagctacgg ctgaactcaa acggttgacg cgctccgcgc ccggctctcc
     5581 gctggagcgt cgtgcacgcg aagcctgtgg gacccgcgtc aactagtcct gagcgtttct
     5641 gctgagcgtt tctgctgagc gtttctgtaa gggcactcgt tcaacgcgcc ttggtcttca
     5701 acgcgccttg gcggcgacga cgttcacctg cacgaactgc ccgaagttca gcgacccggg
     5761 caggccttcg agcgtgtatt tttcgtaatt cacgatctct cgctcgttgc tgtcgctgca
     5821 ttcgagtgtc actttggctc cggccgcgat gggaatcgtg attgggtaat cgagcttgaa
     5881 tatgttgtgt gcgcccttct gatagtcgtt caagaagtac gtttgcggag gggctgacac
     5941 cgttatgctc cactgttcgt agtccgcttg tttgaccgtg ccgccaatcg tgtacggcac
     6001 cttacgccac atctctgact tgtaaacgaa ggtgctggtg tctggccggc ttcccccgtc
     6061 gatgttcgta ggctcgacga caccacggat tcgtagcgtt acatcgtaaa cctcgtttgc
     6121 gtctccgccg acggcgactt ccttgctcgc tctgaagcga ttgttggtga gggtcgtgtg
     6181 attgcacgtc gctccgtccg tgatcgacgg agttcccgaa cacggatcat cgatgcgcag
     6241 cccatcgatc cctcgcaccg cactcgcggg ctctccagcc gtttgccctg ctcctcctgg
     6301 cgagctcccg ccgctcgaca tcgcacctcc ttcggagttg ggcgcgcctc cggaaacagc
     6361 tcctccggcg gtcattcccg ccgcggcatc cgtggtccct ccgctggcgg tgacgccgcc
     6421 cgtcggttga gcgccacctt gagccgcccc ggcggcggga gcgcctccac tcatcgcgtt
     6481 ggagctcccg cccgcgggcg atgtcgcgcc gccaccagaa ctagggtttg tgccgtgcga
     6541 tgcagagacg ccgccggaag aggccgctcc actactggag taggcgccgc ctaccggctg
     6601 cactccgccc gtgccggcgg tgctgatcga ggtacttgca actcccgaac cgaagccgcc
     6661 ggacgcgaaa ctcttggttc caggctgatc cgaagactcg gtcgagcaag cgaaaacaat
     6721 actgtggacc gctgccagaa cgagcacgtg gctcgaagtc cgattgagta ccatcgcgtg
     6781 ctccgtctcc aatgcagttt tgaatcaggt gctttgaaaa aggccaaacg caaggagaga
     6841 cggagattca gttgcgctca cggcccggag agtgcttcag gcctgaatcg cgctcaaggt
     6901 cttaccgccg ctcgctccga agttcgtcgt cggagtgccg gttgcggcac caatgacgac
     6961 attcaacagt gtgccgttgt tttgtccacc gccgtccacc agttggccct gcttgatcgc
     7021 gccccctccg ctgccccaca gaatgaaggg cacgtttcgc atgctgtggt ttccctcggc
     7081 gacgtggttg gtccaaagca cgatgctctt gtcttgcagg ccgcgagctt tgaagtgctc
     7141 gaggccgcgc gccagcgtct gcattctgag ttggtcaatc tctgcgtgtg cggcctcggc
     7201 ggtggaattc tggccgatgt tcccgtccga ctgcgtgcgg tggctgatgt agtgcaagtt
     7261 ccaattgccg agcgtctgat tcgacggcac ttgatagacg gtgtgatcgg tgccgtcgcc
     7321 ccactgcaag gtgccggtac ggttcaaatt acacgcgaag gctagggcaa ccatttgcag
     7381 gtgcagctcg acgatgtttt cgatcccgcc attcgctgcg ttggttggat tgtacttcca
     7441 actcttgagc gcgtcgtaga cgctcgtgtc cacgccgtcc atcgcgcagc ctacggccgc
     7501 catctgcgtg cccatcgtca tcatcgtccc ttccacatcg cggatggaat cgaagtgctg
     7561 ttgcaagcgt tgcttgtcgg cggcgctgag cttgggattc tgaagcagcg agttgagctc
     7621 gcgacgtacg aggtcgttca cactcttgcg cttcttgacc aactcctgag ccatcgggtt
     7681 ggccccgctg ctcgtgccgg tgccgccggt ggtcccgccg cccgcgggcg acgccaatcc
     7741 agtcagtctt tggtaaagct tgtagggatt atcgactgac gcccgcactt gccccttgtc
     7801 gtcgaacgac agccggtcgg caatatagcc gtttttcacg ttaccggcgt acagcgttag
     7861 gggttcggtg ccagagggat tgacggcctt ggagatcacc caatccgctg acggcccggt
     7921 tgccgtagct gcgccaccgt ttccggtcgg ggtcttggcg gtgagcgcct gggacaggcc
     7981 ttgcgcatgc ccgcagccgg tcgggccagt ctggggaaag ttgacattct tgacgatcag
     8041 caagttagcg gagtgagctg cgagtgcatc gaccgccttg ccggatgtga gtagcccgct
     8101 cagcgctccc gagccggatg gccagaagcg ttttggctcg accccacacg ccgcacagat
     8161 gaagaacgtg aacaccggcg ctgagcccgc ggcccacgcc gagcgctctg gcaggccttc
     8221 caggaagggc agagccagcg cgacgccgcc tgctcctcgt aagaacgcac ggcgattgat
     8281 caccgggcga atggggcttc tggcgttcac tgcgcacctc caacgtgcgt gcgaaaagcg
     8341 tcggtcttca cgagcgcgat catgacctct ttgagggagg ccccggttgc aaggctcgtc
     8401 gcgccgagtg cctcgatcag cggtcgctcc gtgtcgatca gatctcgacc gagcgcgtag
     8461 ctggccagct tctttgcgta gcactggtgg gcttgcgtgc cgctagcgat aatctgcatg
     8521 agctccggtg ctccagaaaa ggacttcgta ccttcggcga atggatagcg gccctgggcg
     8581 tcgacgtttt ggccgttgtc catctcccgg aagcgaccca cgccatcgta gttttcgaac
     8641 gcaaacccga ggggattgat gatttcgccg tgacaagtag cgccgcagcc ctcggtgagc
     8701 gcctggtagc gctggcgatt ggtttggttg gcttgaagtg gggggactgg cggaagattg
     8761 accgtgggca aacccggatc cgcgcacaac gtgtccaagt tgatgcgcac cccgcggtga
     8821 accgagtccg gatcgttgtt gatggcccac aaagtcagga acgggagctg ggagtaccaa
     8881 ccggcacgat tctggagagt caccttttga acgccgctgc cctgcgtaga gacgccatag
     8941 agggccgcca tcttcggccc tgcaaagccg acgctggtgg tgagaatctc cttcagaccg
     9001 aggttttgcg tgaagatctg atcgaagaac cggtaggagg cttctttgag ctctgggagc
     9061 aaggcttcgg tgtagccctg cacgcccgtc ttcacgatgg tgtccagcac ctcgattttg
     9121 tagagctgcc cgtggaactt gcgcatcacc gtggtacttg ccgcatcatc gagcatcgac
     9181 ttggcctgcg cggcagcccc atccggacta tcgaacgcac cgctctttgc ggcgtcgagc
     9241 atgctctcgg tgggagtagt atcgcggatc cagagcgaga gcttggccgc gagctcgtaa
     9301 cccgacagca gcttgcccgt gtcacccagc tctattcgat acaagaaatg cggagactgc
     9361 agcatggccg cgattacgag cgcggcgccc ttcgtgaagg cgctctgcga cccggaatac
     9421 gtggcgccct cggccaagag tgcctggtaa ctggtcagct cttccgatgt gagatctctg
     9481 cgaaacgcac gacgaccaaa agtctttacg aacgtggctg cgtctttggt ggcgaccaca
     9541 gtctgtagcg ccgcgtcggt ggcggtgacc tgcgcggcaa cagtctccgc cgccaattgg
     9601 aaatcacccc atatcgtatt gttcacgacg agcactcgct cgttgttgtc gaaatccgtg
     9661 gttcccgata cggcgtgcaa aaatccactg gagacaccag tgggcgcgct gagcttcagg
     9721 acgtcccgaa cactgttctc ccattggtca ttcgtgagcc gcagaaaccg gctgtaaatc
     9781 ggagtgccat ccatactgac cgggcgtggc tgccctggtt gaggcggtgt tgacgtgccc
     9841 gcaccgcccg agttgctggc cgcgcccgcg ggcgaatttc ccgcttggcc gccgctcccg
     9901 ctcgttggcc cccctgggct attacctgct ccgctgggcg tcattcctgg agtaccggcg
     9961 cttccgccaa cctccgagat ggagccagtg catccgcagc cgaggacgag cacgaaactt
    10021 tgcgcgagcg cccgtaggcg aggcttatgc atgggatagc ctgtcgtctg gaattgattc
    10081 accctcggag gcagtgactg agtgaacgcg catccgtcat gcagacaggc gatttttgag
    10141 gcacggtgat cgtaacgatc gatcgttacg aacgactgat ccaacaaggc ggtcgagatg
    10201 caatcgcggg gtaactgcag tcgtttgcaa gcaacgatgt ggaacacacc cgattcaaaa
    10261 gacgcagccc aactgtggat tctcagcgac tctataatca gcggaaatga cggcgatttc
    10321 ttgacgggat cgctcatgct cattcactgc attcctcatg cacctccaga gagagaagtt
    10381 cgcctccctg agacactccc ctacacgtgc agggggccct gcaactagca ccggtaccgc
    10441 tgcaatattg aactcagcgc tttgggcctg gctgggcggg atctttgcga tcgtcggttg
    10501 cagaccagac gcgagcacct caccgatccc cggtacggtt caagctggtg gcgcaacggc
    10561 attgccgagc tttggtggct ctgccggcgc aacgaatatg cagtccggtg gaatcacctc
    10621 tgggggtgcc accggagcga cgagttcagg tgggagtggg ctcgtagctg gtaccggttc
    10681 catgcccgca tcgggcggca ccgcatctac cggtggtgct agcggtggtg tcaacaccgg
    10741 tggccagggc accggtaacg cggcaggcat gggcggcgca actacggcgg ggatgaatgc
    10801 gggtggagcg acgaacacgg caggtagctc tgcaggcaac accggcggtg cctcgggcgg
    10861 agctgccggc gctgcttcgg gaggcggttc gtcgaaagcg gtgccgagcg ccggttgcgg
    10921 aaagtccggg cgtccaaacg gaggcgtcgt cacggtcaac aacgatcata tctacacttt
    10981 ccccacgagc tacgacggga ccaagccact tccgctattg attgggtttc atgccgcggg
    11041 caacccaatc gaccaaattc aaaagctcac caatggctcg tctttcgaga gcaactacgt
    11101 gcgagctttt ccgaagagcg caggcaacga gtgggtatac aacacggacc tgaacaaggc
    11161 ctacaaggta tacgacgact tgttggccaa ctactgcgta gataccagcc gcgtgttcgc
    11221 gactgggcat agctctggcg cgcagatgat cgtccaaatt ctagtgcaca aagacgcggc
    11281 cgatcacttc aagttcaagg ccgtcgcccc cgtggc
//
LOCUS       DASTKU010000534         5008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1890179, whole genome shotgun sequence.
ACCESSION   DASTKU010000534 DASTKU010000000
VERSION     DASTKU010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1890179"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VFQ61_18570"
     CDS             <1..828
                     /locus_tag="VFQ61_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956520.1"
                     /translation="TPGLLERGPITVFEKASFLGQGMLEPLPPRATATVPFALERSVA
                     IGRDFQSDSVGSRIYRVATGQLWIERDSVNRSIYKIDNGGERNEKLLIKHPRTPGTRL
                     FKPPAGTEDNEGSGNALIPVALPAHAKTQFTVEERRSGQETVDWLGPLADDAIKAYVA
                     DSRANPDISKKLSAAWGIRETLRRTNEELQKLNDERAQLESASDENRRNLKAIEKNPQ
                     AADLRAKLTRRLAEGAARLDAITKRTIELEMVRNEQGVRFRDAVGEIHIDGPLPPRD"
     gene            1310..1855
                     /locus_tag="VFQ61_18575"
     CDS             1310..1855
                     /locus_tag="VFQ61_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956521.1"
                     /translation="MFGSLGCARDAGRLASGFTCCSQLLTKLFALLLAAGALGFASES
                     LSFTSLQLFFPNLLLFVASLLSGLSGLFGSFTSFASLLCSLFSSLRCSGRTLASTTCV
                     AGVDGALCAPFDGINRQCDRSLDFCRYRPLGDAREVGDDTIDGTSEIAAVRHVLAPLS
                     HTLLCVVKTNTEFWSNTLSFA"
BASE COUNT          836 a         1527 c         1518 g         1127 t
ORIGIN      
        1 acccctgggt tgctcgagcg cggcccgatt acggtgttcg aaaaggcctc tttcctggga
       61 cagggcatgt tggagcccct cccgccgcga gctaccgcga cggtcccgtt cgcgctggag
      121 cgcagcgtcg caattgggcg cgacttccag agtgacagcg tgggttcccg gatttaccgc
      181 gtcgcaaccg gtcaactctg gattgaacga gatagcgtga atcgttccat ctacaagatc
      241 gacaacggtg gtgagcggaa tgaaaagctc ctaatcaaac accctcgtac cccgggcact
      301 cgcttgttca aaccgcccgc cgggacggag gataacgagg gttctggcaa cgcgctcatc
      361 ccggtggctt tgccggcgca tgccaaaacg caattcaccg tcgaggagag gcgttcgggt
      421 caggagaccg ttgactggct cggcccgttg gcagatgacg caatcaaggc ctatgttgcc
      481 gattcgcgcg cgaaccccga tatatcgaag aagctgagcg cggcgtgggg aatccgcgag
      541 actttgcggc gtaccaacga ggaattgcaa aagctcaacg acgagcgggc ccagctcgaa
      601 tcagcaagtg acgagaatcg gcgcaatctg aaagccatcg agaagaaccc gcaggccgcc
      661 gatctccgcg cgaagttgac tcgccgtctg gccgagggcg cagcgcgctt ggatgcaata
      721 acgaagcgca ccatcgagtt agagatggtg cggaatgagc aaggcgtacg tttcagggat
      781 gcagtgggcg aaatccacat cgacggaccg ctgccgccgc gagactgagc cggcccttgc
      841 atgcgttcac cctagcgctc cccaagcgct agggatgtgc ctgccgaata ccgaaagagt
      901 atctggtcgc acggcgccgt aggacgaggc gcacctgagc ccctgtggaa aacgtttcct
      961 caggggctcg agtgtcaacg ccgcaacgca gcttacgcgc agaagcctcg tcccgcgagg
     1021 cctgtgaagt ctgctggagc cggcaccagg gtgccagctt acccggcagt cactgtcagt
     1081 tggctggagt tgctgccagc gggaccgcgg aatttgcagc ggtcgtgttg ctggaggcag
     1141 cgctgccgtt ggcgatctct ttgcccgtct cggggtcgac gggacgtggc tgcagaacgg
     1201 tgacctgccc gtctctcgat cgcttgaaca cgacgagcgg cggtggttca accttcgggc
     1261 ttatcaagcc gccaccacgc tcagcgcggg cgccgacctt ctcaccctgt tgtttggctc
     1321 gctcggctgc gcgcgcgacg cgggccgcct cgcgagcggc ttcacgtgct gctctcagct
     1381 cctcacgaag cttttcgcgc tcctccttgc ggcgggtgcg ctcggcttcg cgagcgagtc
     1441 gctcagcttc acgagcttgc agttgttctt cccgaacttg ctgctgttcg tcgcgagctt
     1501 gctgtcgggc ctctcgggcc tgttcggcag cttcacgagc ttcgcgtctt tgctctgctc
     1561 gttgttcagc tcgctccgct gcagcggccg tactctcgcg agcacgacgt gtgttgcggg
     1621 cgttgacgga gctctttgcg ctccgttcga tgggatcaat cgccagtgcg atcgctcgct
     1681 cgacttctgc cgctatcgcc cgctcggcga tgcgcgtgag gtcggcgacg atacgatcga
     1741 tggcacttcg gaaatcgcgg ccgtgcgcca tgtacttgcg cccctgtctc ataccttgct
     1801 gtgtgtcgtc aagacgaata ccgagttttg gtcgaacacc ttgagttttg cctgaatgac
     1861 ggcgcgatgc acgtgcgcaa ttggaaaatc gcaacacgcg aacgcgaacg aaggttgtgc
     1921 gttcgcggcg cgtctcgctg ggcgagagga gcgccgagat ggctgtagcc tttcacggtt
     1981 acgaggcgcg tcaaaacgcg cccgttgttg gcttgccgca tttgcggtga gagcgccacg
     2041 ggtcggctgc gagcccgttg ccggccgagt tgacgcagca tccggcgtca tcgagatcct
     2101 gccggtgatt ttcatttcat ctcgtcgcat ctcgtcgctc gctctgtcac gcacgcacgc
     2161 ggacgggctg aactcgcgct atcgctgaag tagcagcggt tcggctccta gctaccgggg
     2221 agtgacacct gtctggtgct tgaattgggt cggcgtatct cggtacccgt gcgaatctcg
     2281 ggatggttca cttggagttg tccacatcca gagttcgact ggctcgagat ggtagcgtgg
     2341 gcccattggc tcgcgcgtac cttttggtat ccaaactgat ggtactgttg ctatccagct
     2401 gacgtgctgt ggctatccag ctgacgtgcc gtggccgcgc tgaaggcaac gcgcctgggg
     2461 cctgccctat cgttctttca tcagggtagc agaggctttg ggttaggcag gtttcagatc
     2521 ggatccgcgg cccgtctggg catttctaca cttgtcttga ggttttgcac cctgtacgtc
     2581 cgctgcgtcc ctggggccgc ttctcgtcga ctccacgtac actccgcgtc cgtcctgcat
     2641 ccactctgcg tccactccgc atgcagactg catgcactct gcatagagcg gctgggctcc
     2701 atacccgccg ggctccatac ccgatacccg ctgggctcca tacccgccgg gctctatacc
     2761 caggagtcaa agtggtgagt atcgttgtat atgtcaagcc gcatttcggc tagtagtgct
     2821 cgctccttcc ttcccgcgag tggattgcga gcgtgcaggg ctctgactgt gtcggttctt
     2881 gcttgggggt agctgtgtcc accgactgga ctcaatccga gaaccgcgga ttgtctgtcg
     2941 agtgcgaccg tcatgctccc gacgtagcgt gctcgaagat gttcgcgtta tgaacagttt
     3001 gccgttcatt gtgcggtggt cgctctctgt cgactgggct cgctgagttc gactgggctc
     3061 ggctgagttc gactgggctc gctcggctgg gctcgctcgg ctgggctcgc tgggctcggc
     3121 ctggcgcttt ggctgctggt ttcaaagccc tctgttcccg atgcctggcc gtgagcgact
     3181 ggtgtatcgt cgccatccta atcggggttg atgggcgaaa gagctgcgga ctcggggcct
     3241 ctgaactgcg gaaccggtcc tacaagcgtt tacttgtgac tctcattctt ccatcatccg
     3301 cgccattctt tcgtcacgag gcggcggcct gacgccggtc aggacacact gcagtgtttg
     3361 atggattgtt ggattgcaag ctagtagcgg ttcgatggct cgggcctttt gactcgttag
     3421 tctgagcgcg ctcgccggtt tgctagccat atcgcggttg cccggcgcat tctgcccagt
     3481 ggaggatatc gtcagttttg ccgattcgcc tcagtacgtg ccagttgcgg ctgccatcag
     3541 ctaggtgctt ccttctcagc gctgagcgct ttcttccaat gtgctgacgc gctttctgac
     3601 taggtgctag cgcactctgg tgcttagcgc attttctgcg ggattgctca ccgtaaacgc
     3661 actctgcggc cggaactccg tacccgagga ttgtgtgctg aggatctttg tgccgactca
     3721 cgttctgctg caaccaattt ctctgtgagc gtgacgaggt ttgttggcat tccgctcgcg
     3781 ctctctaaat cgccgtgctc agatccgcag gctgacttga tcgaatcgaa ccaggaacct
     3841 tttggaggcg taagttcctg actgcggcgc gctcgctgtt caccggacgt gggacctcgg
     3901 cgctaggggc cgcgggttcg tccgaaagcg gacttcgagt tccgaatcaa ccacgaatag
     3961 ctgcggccgg tcgtaggttc gtgttgacga cgaagtccga tggaactcgc accaaggcca
     4021 gtgctggccg atcgattgct ccggtgttgc tttcatcccc gcaaattcaa cttcagttcg
     4081 gcccggcatc gactcctcga aacgcatggc gctcgaaagc gggcacacga caacacaacg
     4141 ttctccgcat cctgtttcgt cgagccctgc ttttcggatc ctgctcgccg cacctattcc
     4201 agcgtgaccc tctgggcctt ggctgtactc cccaatgccg tgctgttccg tcgagccctg
     4261 ttcccattga gtctctattt gattgtccgt ccgaaggacc atcttggctg gagatcgtca
     4321 cccggcgtcg tggctcacga agccgcgagt tcggaactgc tccattcgag cctttccggg
     4381 ctcagtccat tcgagcccgc ctccgtttaa tcgccattcc cgtgcgaggc ccccctgaaa
     4441 gcgactctat tcttagcacc aatcgcgtcg cggttagtcg accaaaatta ctcattcgtg
     4501 ctgtttcttt tcgacacttg gcgagagcac ttccgcgcgg tgcgggcggg ggtgccccgg
     4561 ttgagaccac cccagcgcta acagggttcg ctgacaggcg ctccgttccc gcgctccggc
     4621 actaatgccg ctagccgcga aaatattgct gaaacgcgag ctcaaggcac gacgcgagtg
     4681 cctccaacga cgctatcggc gcccagctgt ggtgaccagg tgtacgcgat ccccccggca
     4741 gctgagcagc cgggagacat tggaacattg cgggtcatga cacggccgag ttgctgcgtt
     4801 tcaatcggtg tgtgtcgggc aagctcctga cgccttatta ctgcagaatc acggattcta
     4861 gagcatcacc gctgggcgcc tcacgaagcg gttttctgcc aaaaggtggc gcaagcggac
     4921 ggaaaacctg aatagcgacc atccccggac acctgtcatc gccttggccg ggtagccctt
     4981 ccggccgact cggcatcttg tggatgca
//
LOCUS       DASTKU010000535        13781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_550696, whole genome shotgun sequence.
ACCESSION   DASTKU010000535 DASTKU010000000
VERSION     DASTKU010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13781
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550696"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..102)
                     /locus_tag="VFQ61_18580"
                     /pseudo
     CDS             complement(1..102)
                     /locus_tag="VFQ61_18580"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
     gene            complement(169..657)
                     /gene="moaC"
                     /locus_tag="VFQ61_18585"
                     /pseudo
     CDS             complement(169..657)
                     /gene="moaC"
                     /locus_tag="VFQ61_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
     gene            complement(700..1059)
                     /locus_tag="VFQ61_18590"
                     /pseudo
     CDS             complement(700..1059)
                     /locus_tag="VFQ61_18590"
                     /inference="COORDINATES: protein motif:HMM:NF021192.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase associated protein"
     gene            1165..2181
                     /gene="moaA"
                     /locus_tag="VFQ61_18595"
     CDS             1165..2181
                     /gene="moaA"
                     /locus_tag="VFQ61_18595"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET9956522.1"
                     /translation="MPSDRPEDLTDRLGRGLRDLRLSVTDRCNFRCGYCMPREHFGPE
                     HPFMPRQELLTFEELQRVARSAAALGVKKLRLTGGEPLLRKDLHVLVGLLRDISGVEL
                     SLTTNGSLLAKQAEGLARAGLDRVTVSLDALEPDLFRRIADADFDVTDVLSGIEAAQA
                     AGLAPIKINCVVQKGLNDSQIVPLARRFRGTGHVLRFIEFMDVGITNGWRLDRVLSGK
                     EILERIAAEIPLEPVPDSEGVATRYRYVDGSGEVGVITSVTRPFCSTCSRLRLSSDGK
                     LFTCLFGNNGVDLRAVLRRGGTDDEIIELMRASWVAREDRYSELRSSRTRSLPRIEMS
                     YIGG"
     gene            2710..3372
                     /locus_tag="VFQ61_18600"
     CDS             2710..3372
                     /locus_tag="VFQ61_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956523.1"
                     /translation="MRLDEVARNLVLATLGVLVLTPACGETEDDEPAAVVGAGGANAN
                     AGNSATNAGRTQQANGGRANAGVGGSREYVCDNVEPVEGESCPQSLTNTFCPSATANC
                     ICEAAPSSEQQQQGRRQQQGGAQLRWTCYSRIGTDGSGGAANGTGGASSQGGTTGTPV
                     AGNSNPGGSGPIDSGGMAGEPNGAGGSGTAGADSNPEGGAPTQGTGNAGDTSVAGSAG
                     SL"
     gene            complement(3475..4833)
                     /locus_tag="VFQ61_18605"
     CDS             complement(3475..4833)
                     /locus_tag="VFQ61_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9956524.1"
                     /translation="MLKLGSIAFSISASALLFACSQPEDDDGAGAGGASAGSGGVLIG
                     SGGMPGLGGSPGLGGLPGQGGSVTLPVGGTPNTGGGNTAGVGNLPGKFEGGTVPINND
                     DYDSLKNSACTGWASEGETLPAQLELVIDVSSSMNQKAPGSNRTKWEVTRDALLEAIP
                     GVTGPGLPPSLAVGLLFYPNLESKISTGAEQDSSACVNTAAAVPMALLGSETAEHRTL
                     VANRISTAQLFRSTPTHDAYEFALNNAVIPSKLSGEKFMLLITDGAPTLSHGCVNPNG
                     TQLTDVNPQPIVEAITRAANEHVRTFLIGSPGSENNRAWMSRAAVIGGTAPAGCQVNG
                     PAYCHMDMTTAPDFAEALREGLAQVVGQVTPCTFTFAEPPAGQEIDPEKINVISTNST
                     GSHSLIVRDDVNNCKGEGWQLTGDQEIKLCPTTCSQVKADVGATIDVVFGCGSIPLPP
                     PR"
     gene            complement(5171..6793)
                     /locus_tag="VFQ61_18610"
     CDS             complement(5171..6793)
                     /locus_tag="VFQ61_18610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9956525.1"
                     /translation="MSDRPRSSDLGIALLLWVAYAGVLGVTVPAVGYSASEVGYLQST
                     EAVGDWLGLLIAAPEQALHREMIEKAWASSETCFSAFKLLAAASRQLLSNVPWASPGF
                     AYRLPPMVWGAFGVALIHGWASRVLNRAAGLVAALTFALIPVAFSQAHVAGSPLSIAV
                     TGLLCCYCYVRSLERSGLGWVLATWGAFVLLLQVQPTGWLLVVAFVAHFLLLQGRTFW
                     NQLRRRRLLVPRAWLYVAVFSWPGLLASWPRLWFEPWQRLQSYLDSGVGAAPGNPWVI
                     TAGTVPGISLVLFLTGTVGSLLTLLHTWEHSEAPESSSSILSSASSSELLWLFAAIAS
                     YAMVELNPRGAGSGPDAGGVNVWIGAYPYFALLAARGFDGVCRSFAENSSAEVVPPLI
                     QRLGRLAVGVSVLAGPLVMCVHSYPFGQAFYTPVVGGAPGAANLGLHRTGSSESLALL
                     STYFNEHASAGQTIYLPRDIAAFERLREDGRVREDLQPTSAIGGSEYAPYTWAPKTSA
                     AEYRIWFDYGTTTPAEVVAHDGVPIVWVYERR"
     gene            complement(6790..7743)
                     /locus_tag="VFQ61_18615"
     CDS             complement(6790..7743)
                     /locus_tag="VFQ61_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956526.1"
                     /translation="MAIRPWLWLAIPGLGALELVMHVLLATKAPTEEEFARVAAQLDR
                     LRKPDDLVVVVPAWSEPLARKAWGEERLSLFVRSRMDVSSYAGAVEVSALDQRAPELA
                     SFEEVQRVRAERLLLRRLENPRYVPSLFVFTDQVRPDRMLVGEWNGEAERDCEFVRRK
                     DTDSSANPKFSRGAERRLSEPLPDPQPRFVCTGAKASFVGVTLLAERDMRPRRCILAP
                     APFNGVLRLRFAQVPIGRVLKGYAARLESAQHVLDGTHVAFSAYVGGQRVGRQAIAAE
                     VGFVPFEFAVQHLQRGSAEVVFEVEGERGAGREFCFNAEMR"
     gene            7810..8430
                     /gene="dcd"
                     /locus_tag="VFQ61_18620"
     CDS             7810..8430
                     /gene="dcd"
                     /locus_tag="VFQ61_18620"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HET9956527.1"
                     /translation="MILSDRDLRARIEAGDIVIEPLDDPEVQIQPASIDLRLDRHFLV
                     FRPAHVECLDPREKNLDATSPIEVPEGEAFLLDPGDFVLGATLETIRVPDDLVARVEG
                     RSSLGRLAIVVHATAGFIDPGFEGQVTLELSNLGRCPVKLYPGMRVSQIVFHELTSPA
                     ERPYGQSRGSKYQGQRGPVASRMEGDRRLTPSHLPRPTPSSIPRKR"
     gene            complement(8449..9372)
                     /locus_tag="VFQ61_18625"
     CDS             complement(8449..9372)
                     /locus_tag="VFQ61_18625"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET9956528.1"
                     /translation="MHDRDGLIWYDGKLVPWREATTHVLTHSLHYGLSVFEGIRAHAQ
                     GDGASAVFRLDSHLRRLYGSAHAFGLVIPYSQAELKAAILTTLRENRLSSAYVRPLVF
                     LGPEKIGVSIRGAGVHVVVAAFEWQSYLALDVAERGARVKTASFRRSSVDSTLPRAKV
                     ASSYTNSILSHMEAVQDGYDEALVLDTQGLVAEGSGENLFIVRDGELLEPVLSSALFG
                     VTRDSIATLARELGYQVRSTALTRDDVYLADEAFFTGTAAGVLPIVELDRRRIGDGKP
                     GVITQRLRAAYSDAVTGRDPRHRAWLTPVQP"
     gene            9566..10483
                     /locus_tag="VFQ61_18630"
     CDS             9566..10483
                     /locus_tag="VFQ61_18630"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9956529.1"
                     /translation="MDRLQAMSLFVRVVELGSFTEAARALSLSRARASEAISGLEAAL
                     GARLLHRTTRRLSLTDDGRSYYERARRILSEVEEAELELKTTRSRARGQLRVDVPVAL
                     ARLFLVPRLPELFSRHPELELLVRLQNRPANLVRDGVDCAISYGKPRDPDLVLHRLSG
                     THLVTCASALYLSRRGVPVAPAQLSDHSCIRFLDSETARPQAWIFGEAAETPSLEVTG
                     NLAFNSMEACLEAASAGLGITQVSSSIAQPALDQGQLVEVLPEFRAVGPELYLVHPAN
                     ARASARLRVFIEFARRVFTGVEGSYRAIR"
     gene            complement(10473..12272)
                     /locus_tag="VFQ61_18635"
     CDS             complement(10473..12272)
                     /locus_tag="VFQ61_18635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9956530.1"
                     /translation="MPSSSLPPPAAEVVPRLAIRDPDGWLKIASWTTIIACALAVLTF
                     SFGRDQGIYALVADGMLNGKVPYRDIWDFKPPGIFFVYSLAQVLFGKNMLAPRVLEVL
                     AMLANVAILRRLGGTLFESRVAGLLGAAIAALIHAEFDFWHTGQPETFGATLTLLALL
                     MTVEESPRPRVRMAQWFGVGLCFGAAALLKPPLGGGVLVCAAYAAQQARLRGADLWRC
                     LRDLLWMGLGAVLPVALCLGWFALKGGLKEMSWTLGEFTPGYTLLSWEGRRASDMLYH
                     AIEELFFKFSAVVAAGVIAEAALPPLCRREREGLLLTLGIIAIHVTGIAMQGKFFAYH
                     YDATLPIASLIAGVGLYKLWRRCLVGGLGGALAFFSFVAVAIPMRYAVRDLPQFFWER
                     CWIRYQYILRMAPFDTREAMDAELSYVADYNLEADRKLAAEVVQLTRPDEPVFIWGFE
                     PAVYWLSERQYASRFIYNVAQRSPWQADYARKSMMRDLDRTPPRVLIVQTRDVFPAVT
                     GNTIDSKDSLYDFPELAARIDREYEFVKRVEDFEVMLRRDPNAIRESELTDDPLAPPL
                     FGWPSADKGRRSPSAGQQGEGGSGGAAGSEVTE"
     gene            12540..>13781
                     /locus_tag="VFQ61_18640"
     CDS             12540..>13781
                     /locus_tag="VFQ61_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956531.1"
                     /translation="MRIWRTSCHAPEPEALDWGGRKARVNRRTGKALIREQTNMNARR
                     LFVSALTLSLSGCATSGGGGEEQDTSRVHQAVVATTLQIQLPLEVPLSRLGLVGLTSV
                     TLRNQTSVEGLTLSNASLTVGNEAKTGDLWANGSITVGDRGAVTGDAVARSTVSIPST
                     TPVSGKSYPNSVFTMQPRMEWSYTLPSSYSADVTANAGETKTASPARYRNITAQGTVK
                     LNAGNYFVDNLTLNSGGKLQIPGDGPAPVIYVFNTLTYQGTITDSNAKGERPVIVYLG
                     TAASTIQTQLDFSLIAPNAAVTLAALNGGTKHRGSVIAKQLELAASAQFAARPSNALV
                     PMLAKDPQACSDMIAADAGLAHPERERQRLSEVARYCMGFGTQCEADLVAKINLDYHL
                     AALQLASGTLSPAQYLAISRDR"
BASE COUNT         2725 a         4594 c         4145 g         2317 t
ORIGIN      
        1 caccgggcgc ccttggctat gattgcggac cgtgcctacg aatacgacgg ttgcccccgc
       61 ggtgggctga gcaacgagct cgaccacttc atcgatcgag agctcttcgg cgcgtaaccc
      121 aaagcgccgc gcagttgtcc ctctcgtggg cgtctcttgt ggtgccggat cggcatcgcg
      181 tttgtagtca ctccgaccgc cggctttctc ccgcaggagc gtggggccaa tctgcatcgc
      241 gcgatcgaag gccttcaaca tgtcgtagac cgtgagcgcc gccacgctgg cgccggtcaa
      301 agcttccatc tccacgcccg tgcgatcgag cgcttccacc cggcaaagca cgcgcacact
      361 gcgcgtcggg ggctcgagct cgagccgcac gtcgatgcga gtgactgaca agggatggca
      421 cagcgggatc agctccgagg ttcgcttcac ggccatgatt ccggccagac gcgccgtacc
      481 gagcacatct cctttgggcg catcggctcg ctccagacgt gcaaacgctt ccgcgttcat
      541 tgtcacacga ctgcttgcca acgcgtgtct cagagaaacg cgcttttcgg tcaccgagac
      601 catgcgcgct gcaccgtccg cgctgaggtg ggttgcgttc gcgcttgctc caacaatttc
      661 gtcgtacgcg gcatcgagcc gcgcgctcgc cccgcggtgc ccgagcttca ccaagacgta
      721 gcgttcgagc ggcgtcagcg cactgaaggt gggcacggga agcggccgcg ccggaccgag
      781 tgcaacgagc aactcggccg gaacctgctc gggacccacg tccacccagg caacgcagct
      841 ttcggcaggc gggtttgccg ccccgagcaa agactggact ttcgcgacat cgacctcgga
      901 tgccgcaccc tcttcacaga ccgcggcccg agtagcatgc ggcaaactgc gccacgccag
      961 cagcgacggt ttgcgcccgg agatgtccgc cgctcggcgc gcagaaagcg gcatcaggtc
     1021 cagatcttca ccgatatcct cgaaaatgaa ccgcttcatt gggcgccgga atctaccacg
     1081 ctgcgcagtg cccagcgctg gcaaatgccg gtctctgcgc ttcgaaacct cgagcttcgg
     1141 gagccatgtg ctagccctcc cagtgtgcct tccgatcgcc ccgaagactt gaccgaccgc
     1201 ctgggccgcg ggctccgaga tctacggctc agcgtcaccg accgctgcaa cttccgctgc
     1261 ggctactgca tgccccgtga gcacttcggg cccgaacatc ccttcatgcc caggcaggag
     1321 ttgttgacgt tcgaggagct acagcgcgtg gcccgtagcg cggccgcgct cggggtgaag
     1381 aagctgcgtt tgaccggggg agaaccgctg ttgcgcaaag atctccacgt gctcgtggga
     1441 ttgctcagag acatttccgg tgtcgagttg tcgctcacca ccaatgggtc gttactcgct
     1501 aaacaggcgg agggcctggc gcgggcgggg ctggatcgcg tcacggtgag cctcgacgct
     1561 ttggagcccg accttttccg acgcatcgcg gacgccgact tcgacgtgac ggacgtcttg
     1621 tctggtatcg aggcggcgca agccgcgggc ctcgcgccga tcaaaatcaa ctgcgtcgtg
     1681 cagaagggtc tcaatgactc ccaaatcgtg ccgctcgcgc gtcgttttcg tggcaccggg
     1741 cacgtgttgc gcttcatcga gttcatggat gtggggatca ccaacggctg gcgcttggat
     1801 cgcgtgctga gcggtaagga gatcctcgag cgcatcgcgg cggagattcc gctcgagccc
     1861 gtacccgata gtgagggcgt cgcgacacgg tatcgctacg tggacggcag cggcgaagtc
     1921 ggcgtcatca ccagcgtgac gcgaccgttc tgcagcacct gctcgcgatt gcgcctttcg
     1981 tccgacggca agttatttac gtgcttgttc gggaacaacg gggtcgactt acgcgcggtg
     2041 ctccggcgcg ggggaacgga cgacgagatc atcgaactaa tgcgggcgag ttgggtggca
     2101 cgcgaagacc gatattcgga gttgcgctcg tcgaggacgc gttctctacc gcgcatcgag
     2161 atgagctaca tcggcggctg attgctggag cacgctcgct tgagagcaaa agtttgcact
     2221 cgagttagag ctgatcgcac cgccgcgatc agcgaatgat cgatgccatt cgagctcggg
     2281 cgcctcgtgc tcgtcgcctt cggtacgatc ccacacgtat cgaaacgagc gcacaaatga
     2341 acgacactaa accaagcccg aactgcggac tagtctcgga aggaatcggg ggctgctgct
     2401 aatccacagc agtctttggt gaagcggatg agctaggcgc ggcgcaggac tcgccgtccg
     2461 agcattgata tcgcgcgcac atccactatg ctttgggctg gctttccgcc gcgcccctcg
     2521 aacttcgggg tgccgaaacc tggcaccgat ccgaccgcat tcatccagcg cgctgctggt
     2581 ctagcgaatg ccggtccagc ccatgtgatc cagcagaaat actgggcccg gtgcgaggga
     2641 cagccgaaaa actcaaccag cacgaaatga gctctgctag tcagagcgac gcaaccaagg
     2701 agtaatcacg tgaggctcga cgaagtagct cgcaacctgg tcctggccac gctcggtgtt
     2761 ctcgtgctga cgccggcctg cggcgaaacc gaagacgacg agccggccgc ggtggtgggc
     2821 gcgggcggag ccaacgccaa cgccgggaac agcgccacca acgccgggcg cacgcagcaa
     2881 gccaacgggg gccgagccaa cgctggcgtc ggcgggtcgc gcgaatacgt ctgcgacaac
     2941 gtggagccgg tggaaggtga atcgtgcccg cagagcctca ccaatacgtt ctgtccttct
     3001 gcgacagcga actgcatttg cgaagcagcg ccctcgtcag aacaacagca gcagggccgc
     3061 cgtcagcagc aaggtggagc gcagctccgt tggacttgct attcgcgcat tggaactgat
     3121 ggcagcgggg gcgcggccaa tggcactggg ggtgcaagca gccaaggagg aaccaccgga
     3181 actccggtgg cgggcaacag caacccaggt ggcagcggcc ctatcgactc gggcggtatg
     3241 gcaggagagc ccaacggcgc gggcggtagc ggcactgcgg gcgcagactc caatcccgaa
     3301 gggggagcgc cgactcaagg cacgggcaac gcgggggata cgtccgtggc tggaagcgcg
     3361 ggctccctct aaaccgcggc tgagagcgct gcggttgcca cgctcgtaga tagatcatcg
     3421 gcctcgaggt catcacctcg gggccgattc tttttggagc cgctcgttgt tcggtcagcg
     3481 cgggggtggc agcggaatgg agccgcaacc gaacacgacg tcaatcgtag cgccgacgtc
     3541 tgctttcacc tgcgagcagg tggtggggca gagtttgatt tcttgatcgc cggtgagctg
     3601 ccagccctca cctttacaat tgttcacgtc gtctcgaacg atcagcgagt gtgatcccgt
     3661 cgagttcgtc gagatgacat tgatcttctc gggatcgatc tcttgcccag cgggaggctc
     3721 ggcaaacgtg aaggtacacg gggtgacctg acccacgacc tgcgccaagc cctcgcgcaa
     3781 cgcctcggcg aaatccggcg ccgtcgtcat atccatatga cagtacgctg gcccgttcac
     3841 ttggcatcca gccggcgcgg tgccaccgat cacggctgcg cgcgacatcc aagcccggtt
     3901 attctcgctg cctggcgacc caatcaagaa cgtgcgcaca tgctcgttcg cggctcgcgt
     3961 gatggcctcc acgatcggct gcgggttcac gtccgtcaac tgcgtgccat tcgggttcac
     4021 gcaaccatgc gacagggtgg gcgcaccgtc cgtgatgagc aacatgaact tctcgcccga
     4081 gagcttgctc ggaatcacgg cgttgttcag cgcgaactca taggcgtcgt gagtcggcgt
     4141 gctgcggaac aactgcgccg tagaaattcg attcgccacc agcgtgcggt gttctgcggt
     4201 ttccgagccg agcagcgcca tgggcaccgc cgcggccgtg ttgacgcagg ctgaagaatc
     4261 ctgttcagct ccggtgctga tcttgctctc gaggtttgga tagaagagca gaccaaccgc
     4321 gagtgaggga ggtaatcccg gaccggtcac gccgggaatc gcctccaaca gcgcgtcgcg
     4381 cgtgacttcc cacttggtgc ggttcgagcc cggtgccttc tggttcatcg agctgctcac
     4441 gtcgatgacg agctcgagct gcgcgggcaa cgtctcgcct tcgctcgccc agccggtaca
     4501 ggccgagttc ttgagcgagt cgtagtcgtc gttattgatg ggtacggtgc cgccctcgaa
     4561 cttaccgggc aggttgccca cgccggctgt attcccgccg cccgtgtttg gcgtaccacc
     4621 cactgggaga gtcacggacc cgccttgacc cggcaatccg cctagcccgg gtgatccgcc
     4681 cagacccggc ataccacccg agccgatcag cacgccccct gaacccgcag atgctccgcc
     4741 ggctccggcg ccatcatcgt cctcgggttg tgagcaggcg aacagcagcg cggaagccga
     4801 aatcgaaaaa gcaatggacc ctaacttcag catagctgta accttcaacg tgagtagtgc
     4861 gccagtcgtc ctactttagt cacttcgacg cgcgagcgaa aggcaacccc taggagcctg
     4921 aacgcccgcg gcgacgctga gtaaagagcg actcctatca aagagcaaag cgacgccctc
     4981 aaagagcaaa ctcatgctcg attcgggcgg gcctcatatg aactagcgtg atgaaagcac
     5041 gagcgtgtga gcgtgatgtg tgagcgctgc atgtcgcgtt cgcgccgctg tggagaatca
     5101 gcatagcaaa tcgcatgagc gccgtcccgt ggtgctccca attttcgtga tgcttgttcc
     5161 ccgcgcacga ctagcgtcgc tcgtaaaccc acacgatggg gacaccatcg tgtgccacga
     5221 cctcggccgg cgttgtcgtt ccgtagtcga accagatgcg atactccgcg gcggatgtct
     5281 tgggagccca ggtatacggc gcgtactcgg agccgccgat cgctgatgta ggttgcaggt
     5341 cttcgcgcac acgtccatcc tcgcgaagcc gctcgaaagc agctatatcc cgcggcaagt
     5401 agatggtttg gcccgcgctc gcgtgctcat tgaagtacgt gctcaagagc gcgagtgatt
     5461 cgctcgaccc tgtccggtgc aggcccaagt ttgcagcacc cggcgcgcct ccaactactg
     5521 gtgtatagaa ggcctggccg aacgggtacg agtgaacaca catcacgaga ggccctgcca
     5581 gcacgctcac gcccacggcg agcctgccga gccgctgtat caatggtggg actacctctg
     5641 ccgagctgtt ttcggcaaag ctgcgacaga caccgtcgaa cccgcgggcg gccaacaggg
     5701 cgaagtaggg ataggcaccg atccaaacgt tgactccgcc agcatctggg ccggatcccg
     5761 ccccacgggg attgagctcg accatcgcat agctcgcaat cgccgcgaat aaccacaaga
     5821 gctccgacga actcgctgag gaaagaatag aagagctgga ctcgggagcc tctgagtgtt
     5881 cccaggtgtg cagcagcgtg agcaagctac ctaccgtgcc agttagaaac aagaccagac
     5941 taatcccggg caccgtgccc gcggttatca cccacgggtt tcctggcgca gcacctactc
     6001 cactgtctag atagctctgt agccgctgcc acggctcgaa ccaaagtcgc ggccagctcg
     6061 cgagcaagcc aggccaagaa aacaccgcca catacagcca agcccgcggc accaagagcc
     6121 gcctgcgtct cagctggttc cagaacgtgc gcccttggag cagcaggaag tgtgcgacga
     6181 aggcgacgac caacaaccac cctgtaggtt gaacctgcaa cagcaacacg aaggcgcccc
     6241 acgtcgcgag tacccagcct agaccgctgc gttccagaga gcgcacgtag cagtagcagc
     6301 agagcaaccc cgtcacggcg atcgagagag gcgaccccgc gacatgggct tggctgaacg
     6361 cgactgggat caaagcgaaa gtgagcgccg cgaccaaacc cgcggcgcga ttcagcacac
     6421 ggctcgccca gccgtgaatc aaggctacgc cgaaagcacc ccacaccatg ggcggcagcc
     6481 ggtacgcaaa ccccggtgac gcccacggca cgttcgagag cagctgtcgg gaagctgctg
     6541 caagcaactt gaaggccgag aaacacgtct ccgatgacgc ccaggccttc tcgatcatct
     6601 cgcgatgcag agcttgctct ggagcggcga ttagcagacc tagccagtct cccacggctt
     6661 cggtggactg cagatagccc acttcactcg ccgaatagcc gacggcgggc acggtaacgc
     6721 cgagcactcc cgcgtacgcg acccatagca atagcgcaat cccgagatca ctcgagcgcg
     6781 gccgatcgct catcgcatct ccgcgttgaa acagaactcg cgcccggcgc cccgctcacc
     6841 ttccacctcg aacaccactt ccgcggagcc gcgctgcaag tgttgaacag caaactcgaa
     6901 gggtacgaag cctacctccg ccgcgatggc ttggcgtcct acgcgttggc ctcccacata
     6961 agccgagaac gcgacgtggg tcccgtccag aacgtgctgc gcgctttcta gacgcgccgc
     7021 ataccccttc agcacacgtc cgatcggcac ttgcgcgaac cgcagacgca gcacgccgtt
     7081 gaagggtgcg ggagcgagga tgcagcgacg cgggcgcatg tcccgctccg ccaacaacgt
     7141 cactcccacg aaggatgctt ttgcccccgt gcagacgaag cgtggctgtg gatccggcag
     7201 aggttccgac agacgtcttt ccgcgccgcg actgaatttg gggttcgctg acgagtctgt
     7261 gtctttgcgt ctcacgaact cgcaatctcg ctccgcctcg ccgttccact cccctaccag
     7321 catgcgatcg gggcgtacct gatcggtgaa cacgaacagg ctcggcacgt atcgcggatt
     7381 ctcgagccga cgcagcaaga gacgctccgc tcgtacgcgc tgaacttcct cgaacgaagc
     7441 cagctcgggc gcgcgctgat cgagcgcaga aacctcgact gcacccgcat aactcgagac
     7501 atccatgcga ctgcgcacaa acagcgacag ccgctcttcc ccccaagctt tgcgtgccag
     7561 aggttcggac caagcgggaa ccaccaccac caaatcgtcc ggtttccgta gccgatccag
     7621 ctgcgccgcg acgcgggcga actcttcctc ggtcggcgcc ttcgtcgcga gcagcacgtg
     7681 catgaccagc tcgagcgccc cgagcccggg aatcgccagc cagagccaag gtcggatcgc
     7741 catgcaccga cgggcagcgt gccacaaaat cgcttccccc ggttaggcag caccgcgata
     7801 atgtgtgcga tgattctctc cgatcgcgat ctgcgagccc gcatcgaagc cggggacatc
     7861 gtcatcgagc cgctcgatga ccccgaggtc caaatccagc ccgcaagcat cgacctaagg
     7921 ttggaccgac atttcctggt ttttcggcca gcccacgtag agtgcctcga cccgcgtgaa
     7981 aagaacctgg acgcgacgtc gcccatcgag gttcccgaag gagaagcctt tttgttggac
     8041 ccgggcgact tcgtgctggg ggcgacgctc gagaccatcc gcgtcccgga tgatttggtg
     8101 gcgcgagtcg aagggcgaag cagcctcggg cgcttggcga tcgtcgtcca cgccacggcc
     8161 ggtttcatcg atccgggctt cgaggggcag gtcacgctag agctctcgaa cttggggcgt
     8221 tgcccggtca aactctaccc gggcatgcgg gtgagtcaga ttgtatttca cgaactcacc
     8281 tcccccgcag aacgccccta cggtcaatcg cgcggcagta agtatcaggg ccagcgcggc
     8341 ccggttgcga gccgcatgga aggcgatcga aggctcacgc cgtcacattt gccgaggccc
     8401 acgccgtcga gcatcccgcg caagcggtga gccggcaccg cgctgcgctc aaggctggac
     8461 cggggtgagc cacgcacgat gacgaggatc gcgcccggtc accgcgtcgg aatacgcggc
     8521 cctcaggcgc tgcgtgatga cgcccggttt gccatcgcca atgcggcggc gatcgagctc
     8581 gacaatcggg agcacgcccg ccgcggtgcc cgtaaagaag gcttcatcgg cgaggtaaac
     8641 gtcatcccgc gtgagcgccg tgctgcgcac ctgataaccc aactcacgtg cgagcgtcgc
     8701 gatcgaatcg cgggtcactc cgaacagcgc actggacagc accggctcga gtagctcgcc
     8761 gtcccgcacg atgaatagat tctcccccga gccttcggcc actaggccct gtgtgtccag
     8821 caccaacgct tcatcgtagc cgtcttgcac ggcctccatg tgggacaaga tggagttggt
     8881 gtaggaagag gcaaccttgg cgcgcggcaa cgtcgaatcc accgagctgc gtctaaacga
     8941 ggccgttttc acgcgcgccc cccgctcggc cacatccaga gcgagataac tctgccactc
     9001 gaacgcggcc accacgacgt ggaccccggc gccgcggatc gacactccga tcttctccgg
     9061 tcccaagaac accaacggcc tcacataggc agagctcagc cggttctcac gcaacgtcgt
     9121 caggattgcc gctttgagct cggcctgcga atacgggatg accaaaccaa aagcgtgcgc
     9181 cgatccgtac agacggcgga ggtgagaatc cagccgaaat actgcgctcg cgccgtctcc
     9241 ctgcgcatga gcgcgaatcc cctcgaacac cgataaacca tagtgcaggg aatgagtgag
     9301 aacgtgagtg gtagcttcac gccaaggcac gagcttcccg tcataccaaa tcagtccgtc
     9361 acgatcgtgc atagacaact ccatccgcgc cacgcaccca tcaccgccaa cccgtagacg
     9421 gttcgatggg ttgcgaccaa agacgcgaag cgcagcctag cctgggaata cccacggaga
     9481 agcacgacct cggagacctc actgttcggc atttccgaac aatgccgcgg tgctccgcac
     9541 cttgcagtgc tatcctcacc cctccgtgga tcgcctccaa gccatgtctt tgttcgtgcg
     9601 cgtggtcgag ctcggcagct tcaccgaagc cgcgcgggcg ctctcgcttt cgcgcgcgcg
     9661 cgcgagtgaa gccatcagcg ggctcgaagc cgcgctcgga gcacggttgc tccatcgcac
     9721 cacgcgccgc ttgtcactca ccgacgacgg acgcagctac tacgaacgtg cgcggcgcat
     9781 cttgagcgag gtggaagaag ccgagctcga gctcaagacc acgcgtagcc gtgcccgcgg
     9841 gcaattgcgc gtcgatgtcc cggtggcctt agctaggcta tttctggtgc cgcggttgcc
     9901 cgagctgttc tcacgccatc ccgagctcga gctgctcgtg cgcctgcaaa accgcccggc
     9961 gaacttggtg cgcgacgggg tcgactgcgc catcagctac ggaaaacctc gcgatccgga
    10021 cctggtcttg catcggctct cgggcacgca cttggtcact tgtgcctcag cgctctatct
    10081 gtcgcggcgc ggggtaccgg ttgcgccggc gcagttgagc gaccacagct gcattcgttt
    10141 tctggacagc gaaacggcgc gcccgcaggc ctggatattc ggcgaagcag cggagacgcc
    10201 gagcctcgaa gtcactggga atcttgcctt taattcgatg gaggcctgtc tggaggcggc
    10261 cagcgccgga cttggcatca cccaggtctc gtcatccatc gcccaaccgg cgctggacca
    10321 ggggcagctc gtcgaggtgt tgcctgagtt cagggcggtg gggcccgagc tctacctggt
    10381 tcatcccgcg aatgcgcgcg cctcggcgcg gctgcgcgtg ttcatcgagt ttgctcggcg
    10441 cgtctttacc ggagtggaag ggtcttaccg agctattcgg tgacctcgga gccagcggcg
    10501 ccgccacttc cgccctcccc ttgctggccc gcgctcgggc tacggcgacc cttgtcagcg
    10561 ctgggccacc cgaatagcgg tggagccagt ggatcatcgg tcaactcgga ttctcgaatc
    10621 gcgttcgggt cacgtcgcag catgacttcg aagtcctcca cccgcttcac gaactcgtac
    10681 tcccgatcga tgcgcgccgc gagctccggg aagtcataca gagagtcctt ggagtcgatg
    10741 gtgttgcccg tcaccgccgg gaagacgtct cgcgtctgca cgatcagaac gcgcggcggg
    10801 gtccgatcca agtcccgcat catgctctta cgcgcgtaat cggcctgcca cggcgagcgc
    10861 tgcgcaacgt tgtaaatgaa gcggctcgcg tattgccgtt cggagagcca atacacggcc
    10921 ggctcgaaac cccaaatgaa caccggctcg tcggggcgcg tgagctgaac cacctctgcc
    10981 gcgagcttcc gatccgcctc gaggttgtag tcggcgacat atgacagctc cgcgtccatc
    11041 gcttcccgcg tatcgaacgg cgccattcgt aggatgtatt gataacgaat ccaacagcgc
    11101 tcccagaaga actgcggcag gtcgcgcacc gcgtagcgca tgggaatggc caccgccacg
    11161 aacgagaaga acgccagcgc gccgcccaag cctcccacta gacagcgtcg ccacagctta
    11221 tataggccca cacccgcgat cagcgacgca atcggcaacg tcgcatcgta gtgatacgcg
    11281 aagaacttgc cctgcatcgc gatgcccgtg acgtggatcg cgatgatgcc gagcgtgagt
    11341 aagagccctt cgcgctctcg acggcacagc ggcggcaagg ccgcttccgc gatcacgccc
    11401 gccgccacca cggccgagaa cttgaagaac agctcctcga tcgcgtgata gagcatgtcc
    11461 gaagcccggc gcccctccca actgagcagc gtgtaacccg gcgtgaactc accgagagtc
    11521 cacgacattt ctttcagccc gcccttcagc gcaaaccaac cgagacacag cgcgaccggc
    11581 agcaccgccc cgagccccat ccatagtaag tctcgcagac aacgccaaag atccgcgccg
    11641 cgcaagcggg cctgctgcgc cgcatacgcc gcgcaaacca acacgccgcc gccgagaggc
    11701 ggcttgagca gcgcggccgc gccaaagcac aacccgaccc cgaaccattg cgccatccgc
    11761 actcgcggac ggggggactc ctctaccgtc atcagcagcg cgagcagagt caacgtggcc
    11821 ccgaacgtct ccggttgccc ggtgtgccag aagtcgaatt cggcatgaat cagcgcggca
    11881 atcgcggcgc ccagcaagcc ggccactcga ctctcgaaca gggttccgcc gaggcgccgt
    11941 aaaatcgcga cgttcgcgag catcgcgagc acctccagca cgcgcggcgc cagcatgttc
    12001 ttgccgaaca acacctgcgc cagcgaataa acgaagaaga tcccgggggg cttgaaatcc
    12061 cagatgtcgc ggtacggcac cttgccgttc agcatcccgt ccgcaaccag cgcgtatatg
    12121 ccttggtcgc gcccgaacga gaacgtcagc accgccaaag cacaggcgat gatggtcgtc
    12181 caagaagcga tcttcaacca cccatccggg tcgcgaatcg cgaggcgggg caccacttca
    12241 gcggcgggcg gaggtagcga tgagctgggc atcctttcgg gcgccacgct aaatcagcac
    12301 aagccgcgga gggcagtcat tctactggcc gcggcccaag agctgctttt ttccgcgccc
    12361 agcgcctacc cttcggaccg aaaccgcccc aggccacccg gcccagttcc ccggtccgtt
    12421 taccgggctg agcgagcaac ccgagcaact cgagcaactc ggaaaacgcg cgcgttcgcc
    12481 tgacccatct gaccgactaa accgcggaga tcgcgagccg aacatggcgg agcgcgctcg
    12541 tgcgcatctg gcgcacgtct tgccatgcac ccgaaccaga ggcgctcgat tggggcggtc
    12601 gcaaagcgcg tgtgaaccga cgcacgggca aggcgctgat ccgcgagcag acaaatatga
    12661 acgcaagaag gctattcgtt tccgcgctga ccctgagtct ctccggctgc gccacatcgg
    12721 gcggtggtgg cgaggaacaa gacacgagtc gtgttcatca agctgtcgtc gccaccacac
    12781 tgcaaatcca actcccgctc gaagtcccac tgtcgcggct ggggctggtc ggcttgacct
    12841 cggtcacgct gcgcaaccag acctcggtcg aggggttgac cctcagcaat gcgtctctca
    12901 cggtgggcaa cgaggccaag actggggatc tgtgggccaa cggcagtatc accgtcggtg
    12961 accgcggcgc ggtgacgggt gatgccgtgg cgcgcagcac cgtctcgatt ccctccacta
    13021 ctccggtctc cggcaagagc taccccaact ccgtatttac gatgcagccg cgcatggagt
    13081 ggagttacac actgccctct tcatatagcg cggacgtcac tgctaacgct ggagagacca
    13141 agaccgcaag ccccgcgcgc tatcgaaaca tcaccgctca aggtacggtc aaactcaacg
    13201 ccggcaacta cttcgtcgac aacttgaccc tgaatagcgg cggcaaactc cagatcccgg
    13261 gagacggccc cgctcccgtc atctacgtat tcaacaccct gacttatcaa ggcacgatca
    13321 ccgactcgaa cgccaaaggc gaacgaccag tcatcgttta cctcggcacg gccgcctcca
    13381 cgatccaaac tcagctcgac ttctccctca tcgctcccaa tgccgccgta accctcgccg
    13441 cgttgaatgg cgggaccaaa caccggggca gcgtgattgc caaacaacta gaactcgcgg
    13501 cgtcggccca gttcgcggcg cggccctcca acgcactcgt ccccatgttg gccaaagacc
    13561 cacaggcctg ctcggacatg attgcggcag acgcgggcct ggcgcacccc gagcgcgagc
    13621 gtcaacgcct gtccgaggtc gcgcgctact gcatgggatt tggcactcaa tgtgaggcgg
    13681 acctcgtagc caagataaac ctcgattatc acttggccgc tctgcaattg gcgtctggca
    13741 ccttatcgcc cgcgcagtac ctcgccatct cgagagaccg c
//
LOCUS       DASTKU010000536        10806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_995942, whole genome shotgun sequence.
ACCESSION   DASTKU010000536 DASTKU010000000
VERSION     DASTKU010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10806
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995942"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFQ61_18645"
     CDS             complement(<1..367)
                     /locus_tag="VFQ61_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956532.1"
                     /translation="MRFGLIHRIMTDALAALGVLALVTSGELDHLFAYGSVIGMVGAL
                     LLPESLHDRPFVRRLSAVLALGVFAIQAARLASGADLLPIAVEFAVALQVIRLATRRG
                     AAHDQQVIVLALIHLIAGTV"
     gene            complement(364..1629)
                     /locus_tag="VFQ61_18650"
     CDS             complement(364..1629)
                     /locus_tag="VFQ61_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9956533.1"
                     /translation="MASPKLANQYPTTPVPLSGGAGARVTPSPVPADGPQSEPPSTRA
                     NREPPPSVPPSEPRAEGKSFFARSFGRLRPPRKLKLTREGKYFIGVTFGVGFAAINTG
                     NNLLYLLLGMLLSLIIVSGVMSELSLRDLSVVRRLPPRAQVGRPHLVEIEVYNHKQRV
                     PSYAIEIEDLRAGQPADKRCFFLKISPRSTQVAAYRRTPARRGRDRHIGFRVATRFPF
                     GLFEKSREISAEGDLIIYPAVDPVTLPQEPRGDRALGNAALGRGSGDEVLGVRPMREG
                     DDPRDIYWRKSTHPQHFVLRERGRETRRDITFQIESTFTGTALPSEEWTARFERRIRD
                     IASRSVAHLKRGDWVTLRTSAGERARSSHAQGADPLLRFLALLEARPEPPRVEPTRAE
                     ANRAVAERAGLGQAALGQAALGQAGERRS"
     gene            complement(1802..2767)
                     /locus_tag="VFQ61_18655"
     CDS             complement(1802..2767)
                     /locus_tag="VFQ61_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9956534.1"
                     /translation="MTSSAHVLEPQNVELLVSLRRAVEGALEGKSEVVELALIALLAR
                     GHILIEDVPGVGKTTLARALARAVGGELRRVQFTSDLLPSDVLGVSVYDQRRAEFVLR
                     HGPIFTNLLLADEINRASPRTQSALLEAMSEGRVSLDGKTVDLPEPFLVIATQNPQDF
                     AGTFPLPESQLDRFMMRLRIGYPPPHVETRLMLDQDIDRIELVPAVLDPRMLVTLQCE
                     VDRVSIDGSLGTYLQALLTATRSSPTLSLGASTRAGMNLSRAARARALLHARHYCIAD
                     DIHTLAVPVLAHRVRLASHAEGYSPSREEAETAVREVVARVPVPL"
     gene            3305..3949
                     /locus_tag="VFQ61_18660"
     CDS             3305..3949
                     /locus_tag="VFQ61_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9956535.1"
                     /translation="MNRIERIEVRNVTRTFGATLALRNVSVSFEPGTLTLLEGPNGAG
                     KSTLLSVIGTVLRPTSGEVVYAPVGSDFEQIRPHIGWVGHDSQCYRELSARENVELAA
                     KIHGLDPEAAWREVVQHVEVSALASRRVGTLSRGQKQRVALARALVHTPSVLLLDEPW
                     TGLDRRSAEQLETVLRAERARGTLVIIVSHGEGTAERLGARRVRIEAGRVVSDG"
     gene            4072..5817
                     /locus_tag="VFQ61_18665"
     CDS             4072..5817
                     /locus_tag="VFQ61_18665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9956536.1"
                     /translation="MLRLFRGGGVAQLVVGGIVVLIIVVFLLEFRAGNRATGHWKVQC
                     AVELKGTCLDQKDFFAAFGLIVPRGADAAAIKRLGLRKQTLEGLIERELLVDAARAMG
                     LGVSEEAIDAELELGRAHVSLPAANAAQLSGQLGLCRPDASRSMCDVGAGLGVRALRV
                     RKTPTDAFDYKLYEREIRNLANRGPREFKEAQERELVAERLRTLVRSRVRVSADEARF
                     VAERAVARTALVSRDWFGKYLVDASPEAVDRWAFENREQVDSAWNTEKANYTEGCPLV
                     REVVVPASAMMVEAGAPSPERKRAEEARARLVAGEEFASVAADLSSGPSALIGGELGC
                     LTKSYGLGAEELMRAVETLKPREISNVVETPRGFHVLQVTGKLEKGQIEQLGRRQVAR
                     GLYVHFASDEAARKFAGELVERVKGGQKLEDSVRELAEKAAQAAGPGIKTAAASAPGK
                     SDKQLLNKSTRALAASERPRFEISAPFGRSGNPLPDVVSKEAIAPLAFELREADRVYE
                     KPIETESGFLVLQLKELTQPDEKERAEVERSLRQYKSDEAVQRYVTELRRKAGTKLKV
                     DASFGEDRTVASDAE"
     gene            5814..7112
                     /locus_tag="VFQ61_18670"
     CDS             5814..7112
                     /locus_tag="VFQ61_18670"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9956537.1"
                     /translation="MRVEKLSELAIERRTLSSTLRLCCVPMPSTHRVVTSLHVRLGSR
                     FEPAPLGGISHFLEHMLHRGTESQPSAHELALAFESLGSELGAATYVDHTVLSASSPP
                     ENLPAVLKLLGEVVRVPVFKDLTIERGIVREEILESLNDEGDPIDPDELALNAAFPDH
                     ALGRPITGTLEALERFDVPTLRQFHEQRYHADAMVLTVAGCVDADRVQQASEVAFAGL
                     RRGELPVSDVPAPLGGPRFRYVEDTGSQTHLRVGFRAPGENDPLEPATELALRILDDG
                     MSTRLYHQVCDERGLAYDVSAAYEAFADCGLFMLAGEAAHTSSEKLLGSFLDVVRELR
                     DNGPSESELTKAQRRFAWQMQAMLDDPAELCAFIGLGELTGIARTPADRLAALESVSP
                     EQVRAALGRVFCAEGLLVASVGKLNKAARRALEKTALAFA"
     gene            complement(7177..7563)
                     /locus_tag="VFQ61_18675"
     CDS             complement(7177..7563)
                     /locus_tag="VFQ61_18675"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9956538.1"
                     /translation="MTTIALRLAEQVRELRVQAGMTQAELAARAGVTVETVARLERVL
                     RGRSSANSNPSLETLARLSQALGVEVAELLSMPSKPKPRDDRLFVILRAATPATRRRV
                     LRVAEALVREDAAETKSDPRTRRRPS"
     gene            complement(7841..8212)
                     /locus_tag="VFQ61_18680"
     CDS             complement(7841..8212)
                     /locus_tag="VFQ61_18680"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET9956539.1"
                     /translation="MSGTAMNSGAEKRSNPGDRSNPGEAAESPTPQAIDLDGLRRAID
                     DVDRRLLDLVTERVRLVLAVGEYKRQHGLAVYDPERERRVLDRLSQAASPPLDADTVR
                     RVFERLIDESRRLEQHHVQRG"
     gene            complement(8395..9600)
                     /locus_tag="VFQ61_18685"
     CDS             complement(8395..9600)
                     /locus_tag="VFQ61_18685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9956540.1"
                     /translation="MLSSFIACHEVEHAPLPPDSWSSTPDNAGAAPGILIPPTTSHYS
                     DNCGGEALIALTEAPIVYFVLDRSGSMAEATGEDAIAKYDAACGAVGDLLEEIGHRVR
                     FGAAIYPANETECDSGVQIFAPTLGDAPASHAPGERGPILINLLKRLRSEIPSGSTPT
                     AAALRRTLALLTGQGKAFVVLITDGAPNCGLRSCDPDACIPDIESVRVDDATCGVDFS
                     CCDPKYLARGSIPNCVDADASESAVAGLARAGIPTYVVGMPGSEVYAQLLERMAVAGG
                     TAQEQAETGYFAVSDRAGLQKALAGIGAQVSLGCRLELTTAPQDLALVNVYLDGNLVG
                     FDEADGWSWLHSAVQPLENPQGGAGGQPEMQRSQELVLELHGEACRALEAGEVRKVEI
                     TYGCQTVVR"
     gene            9798..>10806
                     /locus_tag="VFQ61_18690"
     CDS             9798..>10806
                     /locus_tag="VFQ61_18690"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9956541.1"
                     /translation="MNDNLAPSDPKSPESGPPTTVSARAAQWLLHAGAWLIILIFIGG
                     LRDTPEPVTGAARTAPASQGPRAGRELCVEATRSPGKVIAAAQVHVFEERADGRFTSA
                     GEASTDLRGKACFPQVQPGSVWVLVDAPGRARASSHLILEGDQLVRLELPEASHLSVK
                     VTDELDQAVPQATVLITASDPLPYGRLTDAQGQAQFDWLAKPPFRVRVTAPGYESAQR
                     SSVTADLKIALRRLSSLDVLVTDMQGQPVENAEVWIAGSTLWPARKTETDARGHAPIR
                     GLSAGSFDLVARREDRVCEPVFGFQLGRGAQESLTLRLEPGRFVSARVTDGEAPNAPG
                     IE"
BASE COUNT         1990 a         3496 c         3507 g         1813 t
ORIGIN      
        1 gcaccgtgcc cgcaatcaag tggatcaggg cgagcacgat gacctgttga tcgtgcgccg
       61 cgccacgccg cgtggcgagc cgaatgacct gcaacgccac ggcgaactcc accgcaatgg
      121 gcagcaagtc cgcccccgag gccagacgcg cggcttggat cgcgaacacg ccgagcgcga
      181 gcacggccga gagacgacgc acgaagggtc ggtcgtgcag ggactccggg agcagtagcg
      241 ctcccaccat cccgatcacg cttccgtacg cgaataggtg atcgagctcc ccgctcgtga
      301 ctagagccaa cacgccgagc gccgccaagg cgtcggtcat gatgcggtga atgagaccaa
      361 acctcacgac cggcgctccc ctgcttgacc cagcgcggct tgacccagcg cggcttgacc
      421 cagccctgct cgctccgcca cggctcggtt ggcttccgct cgcgtgggtt ctacccgcgg
      481 cggctcgggc cgcgcttcga gcagagccaa gaagcgaagc agcggatccg ctccctgcgc
      541 gtgactcgag cgagctcgct caccggcgct ggtccgcagc gtcacccagt ctcctcgctt
      601 caaatgcgcc accgagcgcg acgcgatatc gcggatgcga cgttcgaaac gtgccgtcca
      661 ctcctcgctc ggcagcgccg tgcccgtgaa tgtagactca atctggaaag tgatatcccg
      721 gcgtgtctca cggccacgct cgcgcagcac gaaatgctgc gggtgcgtgc tcttgcgcca
      781 gtagatatcg cgcgggtcgt cgccttcccg cattgggcgc acgccgagca cctcgtcccc
      841 cgagcctcga cccagcgccg cgttaccgag cgcgcgatca ccccgcggct cctgcggtag
      901 ggtcaccgga tctaccgccg gataaataat caggtcccct tcagcggaga tctcacgcga
      961 cttctcgaac agcccgaacg ggaaccgggt cgcaacgcga aagccgatgt gacgatctcg
     1021 tccgcgccgc gccggcgtgc gccggtaggc ggccacctgc gtagagcgcg gactaatctt
     1081 gaggaaaaag cagcgtttat cggcgggctg ccccgccctc agatcttcga tctcgatcgc
     1141 gtacgaaggc acccgctgtt tatggttgta gacctcgatc tcgaccaagt gcggccgccc
     1201 cacttgcgcg cgcggaggca ggcggcgcac cacggataga tctcgcaagc taagctcgct
     1261 catcacgccc gacacgatga tcagcgagag caacatgccg agcagcaggt aaagcaagtt
     1321 gttgccggta ttgatcgccg cgaaccccac gccaaacgtc acgccgatga agtacttgcc
     1381 ctcgcgcgtc agcttcagct tacgcggggg ccgcagacgc ccaaacgaac gtgcgaagaa
     1441 ggactttccc tctgcgcgag gttccgaagg gggaacactc ggcggtggct ctcggttcgc
     1501 gcgagtggac ggcggctccg attgtgggcc gtctgcggga actggggacg gggtcacgcg
     1561 agcgccggct ccgccactca aggggaccgg tgtcgttggg tattgattgg ccaacttggg
     1621 gctagccatt cgcttcgcta actctttctc gggtcagact caagcaacag ccaccctcga
     1681 agttcatgcc gaaaagccgc ccgattgcgc atggcatgcc acacccggcc atgctcgctc
     1741 cccttcggga gcctggcacg cgccacagga aagctcatgc cctcgccggg aagttccacg
     1801 atcagagcgg taccggcacg cgcgcaacca cttcgcgcac cgccgtctcc gcctcctccc
     1861 gtgaagggga gtagccctca gcgtgggaag ccaggcgcac ccggtgcgcg agcacgggga
     1921 ccgcgagtgt gtggatgtcg tctgcaatgc agtagtgccg cgcgtgcaaa agcgcccgcg
     1981 ctctggccgc ccgcgacaag ttcatgcccg cccgagtcga tgcgccgaga gatagcgtgg
     2041 ggctcgagcg cgtcgccgtg agtagcgcct gcagataggt gccgagcgag ccgtcgatcg
     2101 agacgcggtc cacctcgcat tgcagggtga ccagcatgcg cggatcgagc accgctggaa
     2161 ccagttcgat gcgatcgata tcttgatcga gcatcaagcg cgtctccacg tggggaggcg
     2221 gatacccgat tcgcagtcgc atcatgaacc gatcgagctg cgattcgggg agcggaaacg
     2281 tgcccgcgaa atcctgtgga ttttgggtcg cgatgaccaa gaagggctca ggcaaatcca
     2341 cggtcttgcc gtcgagcgaa acgcgcccct cactcatggc ctcgagcagg gcggattgag
     2401 tgcgcggact ggcccggttg atctcgtccg ccaacagtag gttcgtgaag atcgggccat
     2461 gtcgcaagac gaactcggcg cgacgctggt cgtacacgct caccccgagc acgtcgctcg
     2521 gcagtaagtc gctcgtgaac tgcacgcgcc tcagttcacc cccgaccgcg cgagccaacg
     2581 cacgtgccag agtagtctta cccacgccgg gaacgtcctc gatgaggata tggccgcgcg
     2641 cgagcagcgc gataagagca agctctacga cctcggactt cccctcgagc gctccctcga
     2701 cggcccttcg caacgagacc agcaattcaa cattttgcgg ctcgagcacg tgcgcggaag
     2761 aagtcatctc ctgagcatcg tagcatctgg ggcctctcgg gctcggtcaa gcagaaaccg
     2821 gctttcaggc atatctactg agtcagtgtt tgtatgtgcg cggccgggac gctgtcccag
     2881 ttcggaaaag cagctcgcac caaaccgggt tgggaaatcg cccggggatc cagaaatcgc
     2941 cacctgtcgg aatgtgcagg tccagcgggg cgttgctccg agccgcgatt caaacgattc
     3001 gatcccttgg aagagctcgc tctcctcgcg ctcgtcgtgt ctcgacgact gggtcgagct
     3061 ttccctgcag cgtcggattt cgctgcgtca agcttctgcc gccgctggcc gggcgtggcg
     3121 gatttttcca aacccaattc caaatccagc cgcccccggc gcgtaggccg atgctccgca
     3181 cttgcggtcc gggcgggttc ggcggaaacg gagttcgacg ccccctgccc aattctggac
     3241 gatcgacagt gctcgacgac cgagttggat gggccgcctc gggacctaga aagaagccga
     3301 gcgagtgaac cgcatcgaac gcatcgaagt tcgcaacgta acccgcacct ttggcgcgac
     3361 cttggcgctt cgcaacgtga gcgtctcctt cgagccaggg accctgaccc tgctcgaagg
     3421 cccaaacggc gctggtaaga gcacactgct ctcagtgatc gggaccgtgc tgaggcccac
     3481 ttcgggcgag gtcgtttacg cgccggtggg cagcgatttc gagcagattc gccctcatat
     3541 cggctgggtg ggccacgatt cgcagtgcta tcgagaactt tcggcccgcg agaacgtgga
     3601 actcgcggcc aagattcatg gcttggatcc ggaggcggcc tggcgagagg tggtgcagca
     3661 cgtggaggtc tcagcgctgg cttcgcgtcg cgtgggcacg ctctcgcgag gacaaaagca
     3721 acgcgtcgca ctcgcccgcg cgctagtcca cacgccgagt gtgctgctgc tggacgagcc
     3781 gtggacgggg ctggatcgcc gcagcgcgga gcagttggag acggtccttc gagcagagcg
     3841 cgcgcgagga acgctcgtca tcatcgttag ccacggagaa ggaacggccg agcggttggg
     3901 cgcgcggcgc gtgcggatcg aagccggccg cgtcgtttct gacggctagc gcacccgtcg
     3961 cgcccgggag ctcggtgtcg atgagcggcg ccgtgggccc ggcggctaaa cggtgcccgc
     4021 cgtggatccc atggggggag cgtggtatgt gcggcgcccc ggtcggtccc catgctgaga
     4081 ttgttcagag gaggcggcgt cgcacagctc gtggttggcg gcatcgtcgt attgatcatc
     4141 gtcgtgtttc ttttggagtt ccgagccggc aatcgtgcga ccggtcattg gaaggtgcag
     4201 tgcgccgtcg agctcaaggg cacctgccta gaccaaaagg acttctttgc tgcgttcggc
     4261 ctgattgtgc cgcgcggcgc cgacgccgcc gccatcaagc ggctggggtt gcgcaagcaa
     4321 acgctagaag gcctgatcga gcgcgagctg ctggtcgacg cggcgcgagc catgggcttg
     4381 ggcgtcagcg aagaggcgat cgacgccgag ctcgagctcg ggcgcgcgca tgtctctttg
     4441 cccgcagcca acgccgcaca gctgagcggc cagctcgggt tgtgtcggcc cgatgcttcg
     4501 cgctccatgt gcgacgtggg cgcgggtttg ggcgtgcgtg cgctgcgcgt acgcaagacc
     4561 cccacggacg cattcgacta caagctttac gagcgggaga tccgtaatct cgcgaaccgc
     4621 ggcccgcgcg agttcaagga agcgcaagag cgcgagctcg tcgcggaacg cctgcgaacc
     4681 ctggttcgtt cgcgggttcg cgtctccgcc gacgaagcgc gtttcgtagc cgagcgcgcg
     4741 gtggcgcgta cggcgctggt gtctcgcgat tggttcggaa agtacctggt cgatgcgtcg
     4801 cccgaggcgg tcgaccgttg ggctttcgag aaccgcgagc aggtggattc ggcctggaac
     4861 acggaaaaag ccaactacac cgagggctgc ccgttggtgc gcgaagtggt cgtgccggcg
     4921 tcagccatga tggtcgaagc gggcgcaccg agcccggaac gcaagcgcgc cgaggaagcg
     4981 cgggcgcgcc tggtcgcggg cgaagagttc gccagcgtag ccgcggatct ctccagcggc
     5041 ccttctgcgt tgatcggagg cgagctcggc tgcctcacga agtcttacgg acttggcgct
     5101 gaagagctca tgcgcgccgt ggaaacgctg aagccgcgcg agatctcgaa cgtcgtggaa
     5161 acgccgcgag gcttccacgt gctgcaggtc accggcaagc tcgaaaaggg ccaaatcgaa
     5221 cagctcgggc ggcgccaggt cgcacgcggc ttgtacgtgc acttcgcctc cgacgaggcc
     5281 gcgcgcaaat tcgcgggtga gctcgtcgag cgcgtcaagg gcggccagaa attggaagac
     5341 agcgtgcgcg agctggcaga gaaggccgca caggccgcgg gccccggcat caaaaccgcc
     5401 gcggcaagtg cgcccggcaa gagcgataag cagctgctca acaagtcgac gcgagcgctc
     5461 gcggccagcg aacggcctcg tttcgagatt tcggccccgt ttgggcgctc gggaaatccg
     5521 ctgccggacg tggtgagcaa agaagctatc gcgccgctcg cgttcgagtt gcgcgaggcg
     5581 gaccgtgtct acgaaaagcc gatcgagacc gagagcggct ttttggtcct ccagctcaag
     5641 gagctgacgc agccggacga gaaggagcgc gcagaggtgg agcggtcctt gcggcagtac
     5701 aagtcggatg aagccgtgca gcgttatgtc accgaattgc ggcgtaaggc gggcaccaag
     5761 ctgaaagtcg acgcaagctt cggtgaggac cggacggtgg cgtccgacgc agaatgagag
     5821 tcgaaaaact atcggagctc gcgatcgagc ggcgcaccct ttcatcgaca ttacggctct
     5881 gctgtgtgcc gatgcccagc acgcatcgtg tagtgaccag cctgcacgtt cgcctcggct
     5941 cccgcttcga gccggcgcca ttgggtggga tttcgcactt cctcgagcac atgctgcacc
     6001 ggggcacgga atcccaaccg tctgcacacg aactggcgct cgctttcgag agcctcggca
     6061 gcgagctcgg tgcggcaacg tacgtggacc acaccgtgct ctccgccagt tcgcctccgg
     6121 agaacctgcc cgcggtgctg aagctgctcg gcgaagtcgt gcgcgtaccg gtgttcaagg
     6181 atctaaccat cgagcgcggc atcgtgcgcg aggagatctt ggaatccctg aatgacgagg
     6241 gagatccgat cgatcccgat gaactggctt tgaacgcggc cttccccgac cacgcgctcg
     6301 gtcgccccat cacgggcaca ctcgaagcac tggagcgttt cgacgtaccg acgctgcggc
     6361 agttccacga gcagcgctat cacgcggacg caatggtgtt gaccgtcgcc ggttgtgtcg
     6421 acgcggatcg ggtgcagcag gcgagtgaag tagcattcgc agggttgcgc cgcggcgagc
     6481 tgccggtgag tgacgtacca gcgccgctcg gtgggccacg tttccgctac gtggaggaca
     6541 ccggcagcca aacccacctg cgcgtgggtt ttcgcgcgcc gggtgagaat gaccccctcg
     6601 agcccgcgac cgagctcgcg ctgcgcatcc tggacgacgg catgtcgacg cgcctttatc
     6661 accaggtgtg cgacgagcgg ggcttggctt acgatgtctc agcagcctac gaagccttcg
     6721 cggactgtgg gctcttcatg ttggcgggcg aggccgcgca cacttcctcg gagaagttgc
     6781 taggctcgtt cctggacgtg gttcgcgagc tgcgcgacaa cggcccaagc gagagcgagc
     6841 tgaccaaagc gcagcgccgt ttcgcttggc aaatgcaggc gatgctcgac gaccccgccg
     6901 aactctgcgc gttcatcggc ctgggcgagc tcaccgggat cgcacgcacc cccgctgacc
     6961 ggctagcagc gctggagagt gtcagcccgg agcaggtgcg agcggcgctg gggcgggtgt
     7021 tttgcgccga gggtctgctg gttgcgagcg taggaaaact gaataaggcc gcgcgacgag
     7081 cgctcgaaaa aacagcgctc gccttcgcat agagcatcgg tgccatgggt gcgtccgtga
     7141 gtgagccgct gcggttcgcg gcggccctcg caaggatcaa ctcgggcgac ggcgtgttcg
     7201 cggatccgac ttggtttctg cggcatcttc gcgcaccaga gcctcggcca cgcgaagaac
     7261 acgacggcgg gtagcaggcg tcgcggcccg caaaatcacg aataacctgt cgtcccgagg
     7321 cttgggcttg ctcggcatgg acagcagctc cgcaacttcg acgccgaggg cttggctcaa
     7381 gcgcgccaag gtctcgaggg aggggttcga gttagctgac gaacggccac gcaagacccg
     7441 ttcgagcctg gccactgttt ctaccgtcac accagcccga gcggccagct cggcttgggt
     7501 cattccggcc tgtactcgca gttcgcggac ttgctccgcc aacctcaacg ctatggtggt
     7561 cactgaacgc tggctcacag actccccagc ccagagcgac tcgtaactta cgatttttgg
     7621 ttaacttgtc atcagaagtc tgcgcctgga cttgaatacc cggctcatcc cgatttcaac
     7681 gcccatcacc ccacgccttg gccggcaatc agccaccgga ccaagcaact atgctcatcg
     7741 atgaaacgat gctcgcccca aaactcccga gacgtggggc aaagagctga atctgccagc
     7801 cacgctgcgc tcctagtgac tactcggcgc agtccatgag tcagccgcgc tgaacgtgat
     7861 gctgctcgag tcttcgcgac tcgtcgatca gtctctcgaa cactcgtctc accgtgtccg
     7921 cgtccaaagg cgggctggct gcctgcgaca aacgatccag aacccggcgt tcccgctcgg
     7981 ggtcatatac ggctaacccg tgttgacgtt tgtactcccc caccgccagc accaggcgaa
     8041 ctcgttccgt aacgagatcg agcaagcgac gatccacgtc gtcgatggca cgccgtaggc
     8101 cgtcgagatc gatagcttgc ggcgtcggag actccgcggc ctcgccggga tttgacctgt
     8161 cgccgggatt tgacctcttc tctgcgcctg agttcatagc tgtgccgctc atgtcacgaa
     8221 cacgaccatg cacgggcaat ggagaacgct cgaagcatgg ctctggacgt gatcctggag
     8281 gaaaccacgt gccatgactc gagaggagtg tggctcgagg aatgcatggc tcggggaggc
     8341 tagctcagac ccgcgcgagt ggcatcactc gatttcgggg ctggccacta aaccctagcg
     8401 aacgacggtt tggcagccgt acgtgatctc cactttgcgg acctcccccg cctccaacgc
     8461 gcggcaagcc tcgccgtgaa gctccaaaac gagctcttga ctgcgttgca tctcgggctg
     8521 accgccggca cccccttggg ggttctcgag gggctgaacc gcggaatgga gccaggacca
     8581 gccatctgct tcgtcgaacc cgaccagatt tccgtcgagg tagacgttca cgagcgcgag
     8641 gtcttgagga gcggtggtga gctctagccg acagcctagg gacacttgcg caccaatgcc
     8701 ggccaaggcc ttctgcaggc ccgcgcggtc ggatacggca aaatagccag tttcagcctg
     8761 ttcctgtgcc gttccccctg cgaccgccat gcgctcgagc aactgagcat agacctcact
     8821 gcccggcatt ccgaccacat aggtggggat ccccgctcga gccagccccg ccaccgcgct
     8881 ctcggaggca tcggcgtcga cacaattggg gatactgccg cgggcgagat acttggggtc
     8941 gcagcaactg aagtccactc cacaagtcgc gtcgtccacc cgaacgcttt cgatgtcggg
     9001 aatgcaggca tcggggtcac agctacgcag gccacagttg ggcgcgccat ccgtgatcag
     9061 cactacaaac gccttgccct gccccgtgag cagcgccagg gtgcggcgca acgccgcagc
     9121 ggtcggagtc gaaccggaag ggatctcgga ccgcaagcgc ttgagcagat tgattaagat
     9181 cggtccccgc tcaccgggcg cgtggctcgc cggagcatcg ccgagcgtag gagcgaaaat
     9241 ctggacgcca ctgtcgcact cggtctcgtt ggccgggtag atcgcggcgc cgaatcgcac
     9301 gcggtggccg atctcttcga gcaggtcccc cactgccccg caggcagcgt cgtacttcgc
     9361 gatggcatct tcccccgtgg cttcggccat gctccccgaa cgatcgagca cgaagtagac
     9421 aatgggcgcc tcggtcagcg cgatcaaagc ttccccgccg caattatcgg aatagtgaga
     9481 cgtcgtcggc gggatcagaa tgcccggagc tgcaccggcg ttatccggcg tggaagacca
     9541 tgaatccggc ggcaacggtg catgctccac ttcgtgacag gcaatgaatg aactcagcaa
     9601 ccccaaaccc agagccccga accaccgaga cgcgccccgg cggactggag cgacgcgagg
     9661 cagcgcgctg gctcgtcgaa acgactgcgc tcgcgttggg gccgaaattc gccgtcgcgt
     9721 agccacgctg gctcggaaca tccggttaga gtagctcagc ccggcgctcg cgaggccggg
     9781 gcactggtcg agtcaacttg aacgataacc tggcaccttc ggatcccaag tcccccgaga
     9841 gtgggcctcc cactaccgtc agcgcccgcg cggcgcagtg gctactacac gctggggcct
     9901 ggttgatcat cctgattttc ataggcgggc tgcgcgacac gccggagccg gtgaccgggg
     9961 ccgctcgaac ggctccagca tcgcaaggcc cccgcgcggg gcgcgagctc tgtgtggaag
    10021 ccacgcgctc cccgggcaag gtgatcgcgg ccgctcaggt tcatgtattc gaggaacgcg
    10081 ccgatggcag attcacgagt gccggcgaag cctccacgga tcttcgaggc aaagcctgct
    10141 tcccacaggt gcagccaggc agcgtgtggg tcttggtgga cgcaccggga cgcgcgcgcg
    10201 catccagtca tttaatccta gagggggacc agctcgtacg cctcgagctg cccgaggcga
    10261 gccatctgag tgtgaaggtc acggatgaac tcgatcaagc ggtgccccaa gctacggttt
    10321 tgatcacggc cagtgacccg ctgccttacg ggcggcttac ggacgcgcaa ggacaagccc
    10381 aattcgactg gttggcaaag ccgccgtttc gagtgcgcgt caccgcacca ggctatgaat
    10441 ccgcccaacg cagcagcgtg actgcggatt tgaagatcgc cttgcgacgg ctctcgtcgc
    10501 tggatgtgct cgtgaccgac atgcaagggc aacccgtgga gaacgcggag gtctggatcg
    10561 ccggctccac cctgtggccg gcgcgcaaaa ccgaaaccga cgcgcgcggc cacgcgccca
    10621 tccgcgggct gtctgcgggc agcttcgatc tcgtcgcgcg acgcgaagac cgcgtgtgcg
    10681 aacccgtctt cggctttcaa ctgggccgag gagcacaaga gagcctaacc ctgcgcttgg
    10741 aaccgggtcg cttcgtgagc gcgcgagtca ccgacggcga ggcaccgaac gcgcccggga
    10801 tcgagg
//
LOCUS       DASTKU010000537         3978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_327168, whole genome shotgun sequence.
ACCESSION   DASTKU010000537 DASTKU010000000
VERSION     DASTKU010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327168"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..686)
                     /locus_tag="VFQ61_18695"
     CDS             complement(93..686)
                     /locus_tag="VFQ61_18695"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET9956542.1"
                     /translation="MKLDFGSPRRLSLPIHQQTEFVLVRPHYPENLGAAARALKAMGF
                     MRLAVVRPGRLCTPEHEMAFKMAVKSWDVLDRAQRYDSVTEAVQGAHCVVATTARRGH
                     SRIQTPRELTPQLLRHAAAGQHITILFGNEKTGLDEDEMKPCNAPMRIPMAASQPSIN
                     LAQAVQLVAYELLIQALDAQDSSAGAGNGEAAPPSEE"
     gene            complement(683..1609)
                     /locus_tag="VFQ61_18700"
     CDS             complement(683..1609)
                     /locus_tag="VFQ61_18700"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9956543.1"
                     /translation="MLLWWLAEADPPAAPPVPRFLYEVRTLRTPSPLMVRISGGTVVV
                     GSTEAEVIVAAGSCAGEPLAHRCTERTFADEYPRRVRTIGDFWLDRTEVSVAEYNRCV
                     AAGRCEPPHYELAAARFEHPDYPVSFVTWADARAYCGFRGARLPTEIEFERAARGPAG
                     RTYPWGNIYNARVANHGRFAVLEQDESDGFAELAPVGSFVSGATPEGVLDLAGNVAEW
                     QADVYRERYDASPPEDFDAPRVTRGGGFLSGAAWLRGAARDYAHPAARRMDLGFRCAR
                     SAGPMADPQKTGLAQVAPLPQTLALSATTAAP"
     gene            complement(1609..2841)
                     /locus_tag="VFQ61_18705"
     CDS             complement(1609..2841)
                     /locus_tag="VFQ61_18705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9956544.1"
                     /translation="MDAGERALPASIGRYQIERLLGRGAMGRVLLAHDPVLDRAVAVK
                     LLRDDLRIPPEQLQGLLHRMRQEARASARVAHPNIVGLFDMGEDPELGLFLVFEYASG
                     STLKERLKQGPLSPPAAARLAREIGDALSTAHSAGVLHRDIKPENIILTKAGSKIADF
                     GIARVPDSTLTRDGGLLGTPAYSAPESITRGDFSPRSAQFSMAATLYEAISQRRAFAG
                     EDAVAVASRITTEEAPPLARACGVPLEVDLVLKRALAKEPSARFESAREFGEKLAEAL
                     DPLPRKPLDTLPDARHRLAAENHAESQATRFALGGAALGLLLGAAGMHLSQGLKSGSE
                     VAPASPASSTTQAPIGWLAEGPPPKPAPQPKIRSAPALPRGAPTVRSDRNELVDAGTP
                     SPIEDAGAPPPDAGLEPR"
     gene            complement(3007..3486)
                     /locus_tag="VFQ61_18710"
     CDS             complement(3007..3486)
                     /locus_tag="VFQ61_18710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956545.1"
                     /translation="MRLNVWIACAGLSGLMAGAAQARVEGNSGYSKSQTFSAAVRLLR
                     VDRGYEIVEKDADAAYLLFKYPVPGQKLVTDGSLEVVEARGAVRVIVTLSKLPEYHEA
                     LLRDALLKKLESELGPAPRPPEKPTKPVDKPGAGDKGGAEKRKKPEGPEPRPDDLED"
     gene            complement(3583..>3978)
                     /locus_tag="VFQ61_18715"
     CDS             complement(3583..>3978)
                     /locus_tag="VFQ61_18715"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HET9956546.1"
                     /translation="IEFHNVDFPYSVHRCFAELRQRGLLPVIAHPERYQPLFKKLDAL
                     EALIDEGAAALLDAAALIGKYGSRPQQTAELILEQGLYHAACSDAHRPDDVQAVARGM
                     QRIEELYGPEELDALFRDGPQALLAGTLP"
BASE COUNT          666 a         1317 c         1298 g          697 t
ORIGIN      
        1 cccgcaagcg accaacgcgc agctcgcgct cgcggcgagg ctggcgagcg tgaacgcgcg
       61 atgaacgcga cgcacttggg ctccccgcgc aatcattctt cgctcggggg agcggcctcg
      121 ccgttccctg cgccggcgct cgagtcctgc gcgtcgagag cttggatcag cagctcgtac
      181 gccactagtt gtaccgcctg cgccaggttg atggacggtt gggaagcggc catggggatc
      241 cgcatcggcg cgttgcaggg cttcatctcg tcttcatcga gccccgtttt ctcgttaccg
      301 aacaggatcg tgatgtgctg cccagcggcc gcatggcgca gtagctgagg ggtgagctcg
      361 cggggagtct ggattcgtga atggccgcgc cgagccgtcg tggccaccac acagtgggcc
      421 ccttgcacgg cctcggtcac ggagtcgtag cgctgcgctc tatccagcac gtcccacgac
      481 tttaccgcca ttttaaaggc catttcgtgt tcgggcgtgc agagccggcc cggtcgtacg
      541 acggctaagc gcataaaccc catggccttc aaggcacgcg ctgccgctcc caggttctcg
      601 ggatagtggg gtctcaccag cacgaactcg gtttgttggt ggatgggcag cgagagacgt
      661 ctggggctac cgaagtctag cttcatggag cggccgttgt agctgacagc gcgagagttt
      721 gcggcagcgg ggcgacctgc gcgagccccg tcttttgagg atcagccatc ggcccggcgg
      781 agcgcgcgca gcgaaacccc agatccatgc gccgcgccgc cgggtgcgca tagtcccggg
      841 cggccccgcg cagccaggcg gcgccactca agaaaccacc accgcgagtg acgcgtggtg
      901 cgtcgaagtc ctcgggaggg ctcgcgtcgt agcgttcccg atacacatcg gcttgccatt
      961 cagccacgtt gcccgccaag tcgagcacgc cctcgggggt ggcgcccgag acgaagctgc
     1021 caaccggtgc gagttcggcg aagccatcgc tttcgtcttg ttcgagcacc gcgaagcggc
     1081 cgtgattggc gacgcgcgca ttgtagatgt tgccccaagg atacgtgcga cctgcaggcc
     1141 ctcgagcggc acgttcgaac tcgatctcgg tggggagcct ggctccgcga aatccgcagt
     1201 acgctcgcgc gtccgcccac gtcacgaagc tcactggata gtccgggtgc tcgaaccgtg
     1261 cggccgcgag ttcgtagtgc ggcggctcac atcgacccgc ggcaacgcag cgattgtact
     1321 cagccacgct gacctcggtg cgatcgagcc agaaatcccc gatcgttctt acgcgacggg
     1381 gatactcgtc ggcgaaggtc cgctcggtgc accggtgggc caggggctca ccggcgcagc
     1441 tcccggctgc cacgatcacc tcggcctcgg tcgaacccac gacgacggtt ccgccgctga
     1501 tgcgcaccat gagcgggctc ggggttcgca gtgttcgcac ctcgtacagg aagcgcggca
     1561 cggggggcgc tgccggtggg tcggcctcgg caagccacca cagcagcatc aacgaggctc
     1621 tagcccagcg tcgggcggcg gcgcgccagc atcttcaatc gggctcggag tccccgcatc
     1681 gaccagctcg tttcgatctg atcgcaccgt aggggcgccg cggggcaggg cgggcgcgct
     1741 gcggatcttg ggttgcggtg cgggtttcgg cggcggtcct tcagcaagcc aaccgatcgg
     1801 ggcttgggtg gtggagctcg cgggggaggc cggagcgacc tctgatccgc tcttcagacc
     1861 ctggctcaag tgcatgcctg cggcaccaag cagcagaccc aatgccgccc cgccgagggc
     1921 gaagcgcgtg gcttggctct ccgcatgatt ctcggccgcg agacgatggc gcgcgtccgg
     1981 cagcgtatcg agcggcttgc gcggcaaagg gtcgagcgct tcggcgagct tttcgccgaa
     2041 ctcgcgcgcg ctttcgaaac gtgccgatgg ctccttggct agcgcacgct tgagtaccag
     2101 atccacttcg agcggcaccc cgcacgcgcg cgcgagcggg ggcgcctcct cggtggtgat
     2161 acgagaggcc actgccactg cgtcctcgcc cgcgaacgcg cggcgttggc tgatcgcttc
     2221 gtagagcgtg gcggccatcg agaactgagc gctgcgcggg gagaagtcgc cgcgggtaat
     2281 cgactctggc gcactgtacg ccggagttcc tagcaatcca ccgtcgcggg tcagggtgga
     2341 gtcgggaacg cgtgcgatgc caaagtctgc gatcttgctg cccgccttgg tcagaatgat
     2401 gttttccggc ttgatatcgc ggtgcaacac cccggcagaa tgcgccgtgc tcaacgcatc
     2461 cccgatctca cgagccaagc gcgcggccgc gggcggggat agtggccctt gcttgagtcg
     2521 ttctttcagg gtggacccgc tcgcgtactc gaaaaccaaa aataggccga gctcggggtc
     2581 ctctcccata tcgaagagcc caacgatgtt cgggtgggcc acgcgggcgg aggcccgcgc
     2641 ctcttgccgc atgcggtgga gcaggccttg cagctgctcc ggcggaatac gcagatcatc
     2701 gcgtagcagc ttcacggcca ccgcgcgatc gagcaccgga tcatgcgcaa gcaacacgcg
     2761 acccatcgcg ccacgtccga gcagacgttc gatctggtag cgcccgatcg atgcaggcag
     2821 tgcgcgctcg ccagcgtcca tgcgcggcac aatctagtca tgcccgaggt acccctgcca
     2881 aggccggaaa cacgtgctcc ggactggctc ttcgaaaatt cacagcttca ctgcgcaaaa
     2941 catggcggcc tgcgcgagaa atcggcacgc aggacatggt cgcagcggct tgggctccag
     3001 gccgccctaa tcttcgagat cgtcgggtct cggctccggg ccttcgggct tctttcgttt
     3061 ttccgcgccg cctttgtcgc cggcgccggg tttatcaacc ggtttagttg gcttttccgg
     3121 cggtcgcggt gcgggcccga gctcgctctc gagcttcttc aacaaagcgt ctcggagcag
     3181 cgcctcgtgg tactccggca gcttggacaa cgtgacgatc acgcggacgg cgccgcgggc
     3241 ttccaccact tcgagcgagc cgtcggtgac caatttttga cccggcaccg ggtatttgaa
     3301 gagcaaataa gcggcgtcgg cgtctttctc gacgatctcg tagcctcggt cgacgcgcag
     3361 caaacgcacg gcagcgctga aggtctgtga cttcgaatac cccgagttcc cctccacgcg
     3421 agcttgcgca gcgccggcca tgagaccact caagcccgca cacgcgatcc acacattgag
     3481 cctcatgacg actcaggtcc tagcgcctca cgcggtccgg gccaacttct cgctccgggc
     3541 gggaccgctc aagccaagcg caggccaggc aattgcttca tctcaaggca aggtgcccgc
     3601 gagcaaagcc tggggaccgt cgcggaacag ggcgtccaac tcctccggcc cgtacagctc
     3661 ctcgatgcgc tgcatgcccc gcgcgaccgc ctgcacgtca tccggtcgat gcgcgtcact
     3721 gcaggccgcg tgatagagcc cctgttccaa gatcagctcc gctgtttgtt gcgggcgaga
     3781 accgtacttt ccgatcaacg cggccgcatc gagcagcgct gcggcgcctt catcaatcag
     3841 cgcttcgagc gcatccaatt tcttgaacaa cggctggtag cgctcggggt gcgcgatgac
     3901 cggcaatagt ccgcgttgtc tgagctcggc aaagcaccgg tgaaccgagt acggaaaatc
     3961 gacgttgtgg aactcgat
//
LOCUS       DASTKU010000538        25941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1444133, whole genome shotgun sequence.
ACCESSION   DASTKU010000538 DASTKU010000000
VERSION     DASTKU010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 25941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25941
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444133"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1915)
                     /locus_tag="VFQ61_18720"
     CDS             complement(<1..1915)
                     /locus_tag="VFQ61_18720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9956547.1"
                     /translation="MAISNTSKVPIGTVLEGKFRITREIGRGGMAAVYEAENIDIGKR
                     VAVKILAAELITSRIVRERFIREARASASIRSPYICDVYDSGMFDDRPFLVMELLVGE
                     SLYDMMTRVRQIDVATTLRVASHTARGLAKAHESNVVHRDLKPENIFLTHDEEGKLLA
                     KVLDFGLAKFYEPTGGDAQSVRLTREGALFGTPAYMSPEQAKGQGEVDHRADLWALGC
                     IVYECLTGQTVWNVEQGVAMILAQIANAPLPRPSKLRPDLPASFERWFERALHRDVDK
                     RFQTAKEFSDSLVEALSGREAADADSGRSELGMPSESRLRRDRRASNDDDDNELTDVE
                     VTSPISARQAVELPTEPEPERSGSLRGLSILMVFAALSLGGYATWLYVLHPPGKPADV
                     AKPPTRPAASTSEARRPTKTDAVLSDAETEPYAKKIAEAQELLRTDPARAVEAFRAAL
                     KEGSQPAARSLLSHAAVAVEEAGDCRVTGVSRPRPFQVDVPVSRPTLAVTTKGIVVSW
                     VDNHFDPKRRQAFTVLLDSAMRRISAPIAVTPEAQNVRQHELVPIGDRLALVYYDDSG
                     RESGVYARLLDSEGKIQTSLRKLNTGRKGDYRPVLAPIPGAGFYAIWEEEFESNSTDL
                     VGRALGPDLEPLGA"
     gene            2363..2701
                     /locus_tag="VFQ61_18725"
     CDS             2363..2701
                     /locus_tag="VFQ61_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET9956548.1"
                     /translation="MKKVEAIIKPFKLDEVKDALSEVGIQGMTVTEVKGFGRTGGKKE
                     VYRGSAYVVDFVPKVKLEIVVPDQMVHQVIDAIEKSAKTGRIGDGKIFVIPIDEAVRI
                     RTGERGEEAI"
     gene            2967..4412
                     /gene="glnA"
                     /locus_tag="VFQ61_18730"
     CDS             2967..4412
                     /gene="glnA"
                     /locus_tag="VFQ61_18730"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET9956549.1"
                     /translation="MQEGNPGLEEQMDPKQAIELGKRNGAVMVDLKFVDLVGKWQHTS
                     VPFQRLTPESFEDGFGFDGSSIRGFQPINQSDMLLIPDATTAVIDPFYKHPTLSFICT
                     VQDTITRQSYTRDPRYVAAKAEAYLKSTGIADTAFFGPEAEFFIFDDVRFAASRHEAF
                     YRIDSAEADWNTGREENPNLGHKIRHKEGYFPVPPQDTQQDLRTEICLTMAQVGIEVE
                     VSHHEVATAGQGEIDMRYDSLLRMSDKLMWFKHIVKNVAHRNGKTATFMPKPMFGDNG
                     NGMHCHQSLWKEGKPLFAGDGYAGLSDTAIYYIGGILKHAHALAAITNGTTNSYRRLV
                     PGFEAPVNLAYSNRNRSAAIRISTYSPSPKSRRLELRFPDPTCNPYLGFVAMMMAGLD
                     GIQNRIHPGDPLEKDIYALSPEELKDVPRMPASLEQALDALEQDHEFLLRGDVFTRDL
                     IEMWLDYKREAEVQASRLRPTPFEFYLYYDV"
     gene            4607..5455
                     /locus_tag="VFQ61_18735"
     CDS             4607..5455
                     /locus_tag="VFQ61_18735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9956550.1"
                     /translation="MSSLIRSTAPSQALLIVLSGPRLGTRSVLGESAVDVGRGSQCHL
                     ILDADSVSRRHARVEWNGQEHRLVDLASTNGTYLNGSRVREAVLKDGDRIGIGKALLK
                     YVAGGNIEAAYHEEIQRLARFDALTGIYNKRQFEESLRFSMLAARTAPKPVSLIVFDI
                     DHFKRINDTHGHTAGDQVLCDVAKVVKEGLGEGYVFGRVGGEEFAILCEGSGLEAAAE
                     HAETIRSRVSQHRFAFEGSAFPVTVSLGVAERTGGGDEEPEKLYERADEKLYRAKTSG
                     RNRVCF"
     gene            complement(5930..7084)
                     /gene="metK"
                     /locus_tag="VFQ61_18740"
                     /pseudo
     CDS             complement(5930..7084)
                     /gene="metK"
                     /locus_tag="VFQ61_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239920.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
     gene            complement(7341..7688)
                     /locus_tag="VFQ61_18745"
     CDS             complement(7341..7688)
                     /locus_tag="VFQ61_18745"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9956551.1"
                     /translation="MLLCIVTCSGGGLACTSERGTIGAMLVQQPNQRLVVKEVPSGLG
                     AAQAGVQEGDEVLLIDGRDPRSMSPREVHQALSGEVGAPVKLTLIRKDQVIRVRVART
                     PAPALRARPTRPD"
     gene            complement(7873..8751)
                     /locus_tag="VFQ61_18750"
     CDS             complement(7873..8751)
                     /locus_tag="VFQ61_18750"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9956552.1"
                     /translation="MGIRGQRYFEEANQLGGLVAKMRLASLSQVTSAEASTRGDSPEL
                     IQKLETALNLVRSEFMMVPDKPAAILAGAVRTPTPATLDMLRGHLNVLLDLFSQRGLF
                     AGNLEATVRRITESAASTLGIERVSVWLLDAPLSQSLHERTLVCADLFERTPAHHSAG
                     TRLNAADFPSYFQSLVSERTIAAHDAHTDPRTACFSAVYLTPLGIGAMLDVPIWASGG
                     MVGVLCHEHLGGTRRWTEDEETFAYFLSNLVTLTLEQRALTNAHTGETSLTTATDGER
                     PRAEAAAGALQPEAAQ"
     gene            complement(8860..9720)
                     /locus_tag="VFQ61_18755"
     CDS             complement(8860..9720)
                     /locus_tag="VFQ61_18755"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HET9956553.1"
                     /translation="MLRDYIELSRPKDWIKNVFVFMPVPFALASGAALKPAVFGLGLT
                     AFCLGSSAVYAFNDALDAERDRAHEKKRKRPVASGRVSLLQAKLWSLALVVASIGLAF
                     ASASMPALAILLVYLTINTAYCLGAKNIALLDVFLLASGFVLRVLLGCALLDVPPSHP
                     MLLCSYALALFLALAKRRADVTKGLDSSHRPSLLGYNQSFLDHAMTITAAMAVVGYAL
                     YSLESNVLIQTRKFASLPFVLFGVLDYMRLVHVRKTGGSPVDLLLASPALLVTGVGWL
                     IAVWWSVKLP"
     gene            complement(10206..10442)
                     /locus_tag="VFQ61_18760"
     CDS             complement(10206..10442)
                     /locus_tag="VFQ61_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956554.1"
                     /translation="MTPSGTLEPRRCASCNKLSPPTVWGFTLLMPEQGWIVRSAEGSC
                     AVWYCPDCSPPLRRAREHGTSEATPSFAVEQVAV"
     gene            11060..11755
                     /locus_tag="VFQ61_18765"
     CDS             11060..11755
                     /locus_tag="VFQ61_18765"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9956555.1"
                     /translation="MTQVDQELPDGWAEALPDDPAFAKFLAEQLPELAADASGHARTD
                     SAQAERESARAGGASAGQASIRGDVTAPTAGVNRLLASVAELPARYAPFFGRIATLWD
                     VSEEVVTAVLDRARLQSAWKRTPLPGVRVLEASGGPRTSGGDVFLVRFAPGTRFPRHR
                     HPGRELMLVLEGSYTDTLGTRVAAGDLHDMGPGSEHGFLVSENEPCIGAVLHFGREFT
                     NPVLRVIDKLLGR"
     gene            complement(11865..13313)
                     /locus_tag="VFQ61_18770"
     CDS             complement(11865..13313)
                     /locus_tag="VFQ61_18770"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9956556.1"
                     /translation="MSLSPSRRTFLRAVGAGFATLPFYRLVENSFAQSVGEALPLKFI
                     GVYHPHGIAAELFTMRASDTETNFNLKYENSPLAPFDDAATYGASFKSKIVVIEGIDL
                     LSNANGHDTAGTILTGSRIDGGQKPQNSSLDQFLAVEKGLGKNTRVTSIALAVGNKEL
                     KAGETLSFGPGGAPISKVIDPVAAYDLLFKGAVTSTDPAAQAAAERQRKLGQSLVDYV
                     NGDVKRLRARLAPNEQQKLDQHLDSLRELEKQFAAPTSGSGGSGSCSPLARPNAFPKL
                     ERYNGGEPYFDAITDAHVDILAQAMACDITRFGTLVLNDLSYANNPLGLPEDNHGGVA
                     HTYSSSAIGSSGNPVGAGNPSTWATLAKFNRYSYSKVARLMQKLNQLGVLDSTLIYAS
                     SDMGNPAGHSTRNAPTLLAGGANGKIRMGRRLKYRTDCPMETWCDMNGPDYQTVPNNR
                     LLVSIAQAFGVQIESYGTQPDTKLTTGKLAEL"
     gene            complement(13310..15172)
                     /locus_tag="VFQ61_18775"
     CDS             complement(13310..15172)
                     /locus_tag="VFQ61_18775"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET9956557.1"
                     /translation="MRISNRWLSRLGIGSCGLVLACSSDPSGLGLGGAAGTSPIPSAG
                     AGGVGNGTGGRSGMGASAGNQTAGSGPATGSAPSNGGAINAGGSTPQSGGQNAGQGGA
                     VSSGGSTQSNGTVPTSALIPARIRRLSNAEYDITVKKLLGANAKALTAEFPPDLRQNG
                     FTVNDAQRVDAVMVRSLSASAEALAAEARKNGTLTQLAPCQNTAQGEACARTFISSFG
                     ARVHRRALANEETDALLALYRVAADGATYQDGVEHVLKGMLQSASFLYLTELGNGGSS
                     GGALTLTQFEIANSISYLIAAAPPDQKLLDKASQGTLDDPAVREEEARRLLGEDAGAQ
                     ERAVRLVREWLGLDRISETSKDAVVYKNWESSKAAIVAESSDFPRRVMFDSSGKLSEL
                     LGADWTVSSAPLGIYKTQGAGPIANSSKVTDRLGILNQAAFLSVYANAHESHPVLRGV
                     ALARRVACISIASPTSLNIQVVPPVPDPAKTTRERFDVHSTDDACKSCHRVLDPLGFS
                     FEQFDGMGAFRSTENNKPVNSAVQISLSTDFDGSYANSNELAKALANSAQVRDCFARN
                     VFRAASASNDAALKPTENAFIENWRGNAVASAGGIVETLITYVKSPLFTQRRMP"
     gene            16641..17717
                     /locus_tag="VFQ61_18780"
     CDS             16641..17717
                     /locus_tag="VFQ61_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956558.1"
                     /translation="MRVSWLAPAAASAALFAGGWVQARTTTKPGQAEPASRGTPTWSN
                     PEPKPEPKKAELKPKAELKPKAEPKPVRRSTEKKPEAKSEAKSVVIECYPACRDGFVC
                     ARGACVSLCNPPCASDQICVDEGKRCEQLPTATSQSLPSPSSSSSSAETPPAPAATAT
                     EPAAAPVEPPIPEPAPPRRKPYYERSYSGLGFHLGLGGSVDQSGSKYTLDSTLGFNIR
                     SDIPISKYFVIGPLFQFAAWRPQAVGLSLSRSYYVDLDLYLRARIPVESESLPIEFWL
                     GAPVGVSLNFLSDPRPAVQNTFSVGWNFGGLIGTALHLRQGFGVFLEGGAAQHRTSYD
                     GAQGARVSLTISQFNFNAGFTFRN"
     gene            complement(17843..18826)
                     /locus_tag="VFQ61_18785"
     CDS             complement(17843..18826)
                     /locus_tag="VFQ61_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956559.1"
                     /translation="MRKNIQLCGLLLGSLTSFASAGCLRPIGEDPFGTDPATGGTATG
                     CGGGTSSATGGAATAGSGNATATGGATGAGAVTGTGATTATTGGSVNAGGTPGSSGGA
                     VGTGTPTGGTTAAGGSSETGGISSSGGSDSSGGTTSVPGNQCGDGVLGDGEMCCTDEW
                     LGQRINAMLGESAKAVNDVHSCIPRDTSVAGVDLCGTAACQGQAVGCDAVATGFTAAY
                     DSATSKMSGTANLTVTGGLSVSLGLISGSCNFTAVINNLTYSADVVIQKTPEAASIAA
                     ENLVMNYTVQMQNCNLPGAQEAVNQVAKTIVEPKIREQLAQAAKGTIQCPY"
     gene            complement(18863..20335)
                     /locus_tag="VFQ61_18790"
     CDS             complement(18863..20335)
                     /locus_tag="VFQ61_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956560.1"
                     /translation="MHADEPSAAEAGGEPGSIVWLRTNRGVIQGSVIQSNTIQGGAMQ
                     GNTSAAAWAVTTPALLCNEAYGATLSENVPWAVTSEGVWIASYGGGLRHIDSSGCVSS
                     TGAEASKNTLEIGRAPIVVMEQFGETLYVVTSASEAEPGRLLASRDAGLTFTRGSDIR
                     DYVTGLASSASKPERLYASAMNLEDPAASQAELLTSDDAGQSFTAHVIPLEASEIRAR
                     VLAVSPDDPNSVFVATQGANQDSDERLLLTRDAGRSFTEVFRGLGTLVLGTANTNGVA
                     DGNETAGASAADGNHTVYWLGNPLGLFRANALGASFEAVTTDVTDVSCLAVHEGALFV
                     CGARGNQAGVFVAPTGDARQLPEASAMSGAQIELTLRPWLLFSQVTQPLVCPGDANNE
                     ELCAESFEDFAREHASSEPLPQNPSVDGSTSAPARSPSVTGGQAAGCRFVPPRSDRSS
                     RTNRLTTLLGSAFLVASWVRGPRRRRVRSQRNLQPNDRKN"
     gene            complement(20446..21984)
                     /locus_tag="VFQ61_18795"
     CDS             complement(20446..21984)
                     /locus_tag="VFQ61_18795"
                     /inference="COORDINATES: protein motif:HMM:NF013264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956561.1"
                     /translation="MTGQVPTRRKVRDSKRHRVQWVCEFAATIAVPFALSCSPTGSDP
                     LSSDPSGQPLVCQPGSSVACTCSSGATGTAACTQDGSAYSACACSAVPSSPGAGVAGN
                     SNSSSGSSGAPPVDAAGGSSGTSGTSSHLASDLPCEINAIVARNCQGCHASSPRFGAT
                     HPLISAADFQGEASSQVLSRVRDAVRPMPPAPNARISDADLAVLESWLNSGAPAGHCE
                     TPTAATDPLVPSSGATDPDEDVKCYSIVARYSSTGEKYPVPLNKDYYQCFEYDRPWGD
                     EKVQLVSARPIIDNAAVLHHWILYNNTDAVVDGAHTSCGGAHPDAANIAGWAPGGEEL
                     HMPPDVGLRIEPGGFTLEMHYSNGTGTLQPDASGAEICVTSKLRKHEAAVHWLGTQNL
                     NKLSATGTCEPSTRVPVTVISATPHMHLQGRHMSTIIHRVDGTTDTLLDVPFDFNSQV
                     TYSTPAIVNPGDTLSTTCDYAMPTPFGAGTNQEMCYNFVIAYPAGGLAPDFQLFRKYN
                     CAGF"
     gene            complement(22164..23105)
                     /locus_tag="VFQ61_18800"
     CDS             complement(22164..23105)
                     /locus_tag="VFQ61_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956562.1"
                     /translation="MRTRRTELVGLAVIMGLGLVTACSGEGGLPIDLGGIGLTPGTGG
                     ASGGASTGGKSSTSGGAQATGGVTQNSGGSTGKAGSTSTGVTGGSSSGASTGSGGATT
                     GSGGSSSGGATSKGGSTGSGGGVGPGGGMGPGGMGPGGMGPGGASSGGRGGGFGGRTG
                     GGGRPGSGGATGSSGGVTASGGASSGGVMNSGGATASGGSTGSGGATSSGPTFAQVNA
                     ILQKSCAFNGCHGESNPAGGFSFTEASAYQTLTSGKAVSGCQNLVIVVKGDVAKSGLA
                     QVINPSGGCRLPMPLGTPLSEAERKIITDWIAAGANP"
     gene            complement(23102..23329)
                     /locus_tag="VFQ61_18805"
     CDS             complement(23102..23329)
                     /locus_tag="VFQ61_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956563.1"
                     /translation="MIRELVRLGRALHGAQSAVTPKHGRQLGSTPGAKAGESTGDANA
                     DCALNQAEREHAKQIEHGAGLTSLLPFGGKE"
     gene            25171..25941
                     /locus_tag="VFQ61_18810"
     CDS             25171..25941
                     /locus_tag="VFQ61_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956564.1"
                     /translation="MSPQVTLALSVLGLAGSGCVELPEPPPEGRDLSARYREYDHPTG
                     EVPDSARGQLFQSALILATSLRAFSGLVVVRSLISDSSVAGAGYAVEGSLVQGSLKAS
                     VDCAKDPMHPEIAGGHVELLLGIRGSRISRALNGAVVDCRLLVTATDVAQVNSDLTID
                     LGAELELGEVLRSPILVRAENVDLGGETSGVHDVNIEFRIQGYDVLESLVDMQIIGIP
                     GTGSVVVQYRRDGWFGVVEKRGIWTCAGTGEPACVLKQ"
BASE COUNT         4632 a         8130 c         8113 g         5066 t
ORIGIN      
        1 gcgcgccgag cggttcgaga tcggggccca gtgcgcggcc gaccagatcg gtcgagttcg
       61 attcgaactc ctcttcccag attgcataga acccggcccc gggaatcggg gccaagaccg
      121 gtcgatagtc gcctttgcga ccggtgttca gcttgcgcaa gctcgtctgg atcttgccct
      181 cggaatccag aaggcgagcg tacacgccgc tctctctgcc actgtcgtcg tagtacacga
      241 gcgccagccg gtctccgatt ggcaccaact cgtgttgtcg aacgttctgc gcctcgggcg
      301 tgacggcgat cggcgccgag atgcgacgca tggccgagtc gagcagaacg gtgaacgcct
      361 ggcgtcgctt cggatcgaag tggttatcca cccaggacac cacgatgccc ttggtggtca
      421 cggccaaggt cgggcgagac actggcacgt ccacttggaa gggtcgcggg cgcgaaacgc
      481 cggtgacgcg acaatctccc gcctcctcga cggcgacggc cgcgtgcgag agcaatgagc
      541 gtgcggccgg ttgcgagccc tccttgagcg cggcgcgaaa cgcttccacg gcccgagccg
      601 gatcggtgcg cagcaactcc tgagcttcgg cgatcttctt ggcgtatggc tcggtctccg
      661 cgtcgctcag cacggcgtcg gtcttggttg gccggcgcgc ttcgctggtg ctcgccgcgg
      721 gccgcgtcgg gggcttcgcg acgtccgcag gtttgcccgg cggatgcagc acgtaaagcc
      781 aggtggcata gccacccagg ctcaaggccg caaacaccat caagatcgaa agtccacgca
      841 agcttccgga ccgctccggt tctggctcgg tgggtaactc gacggcttgg cgcgccgaga
      901 tcgggctcgt cacttcgacg tcggtgagct cgttgtcatc gtcatcgttc gatgcgcgcc
      961 ggtcccgacg caaacggctc tcgctgggca tcccgagctc cgaacgcccg ctgtccgcgt
     1021 ccgccgcttc gcggccggag agcgcttcta ccagcgaatc agagaactcc ttggcggtct
     1081 ggaagcgctt gtcgacgtcg cggtgcagcg ctcgctcgaa ccagcgctcg aagctggccg
     1141 gcagatcggg ccgcagcttt gacggccggg gcaggggggc attggcgatc tgggccagga
     1201 tcatggccac gccctgctcc acgttccaga cggtttgtcc cgtcaaacac tcgtagacga
     1261 tgcaaccgag cgcccacaga tcggcgcgat ggtcgacttc accttggccc ttggcttgct
     1321 cggggctcat gtacgccgga gttccgaaca gtgccccttc gcgtgtcagc cgcaccgact
     1381 gcgcgtctcc gcccgtgggc tcgtagaact ttgccaggcc gaaatccagg accttggcga
     1441 gcaacttacc ctcttcgtcg tgcgtgagaa aaatgttctc gggcttcaga tcccgatgca
     1501 ccacgttcga ctcgtgtgcc ttggcgagcc cgcgcgccgt gtgggatgcg acgcgcagcg
     1561 tagtcgccac gtcgatttgc cgcacccgcg tcatcatgtc gtacaggctt tcgccgacga
     1621 gcagctccat gacgaggaat gggcgatcat cgaacatccc cgagtcgtag acgtcgcaga
     1681 tgtacggact gcgaatcgac gccgaggccc gcgcttcgcg aatgaaccgt tcgcgcacga
     1741 tgcgcgaggt gatgagctcc gcagccaaga tcttcaccgc cacgcgcttg ccgatgtcga
     1801 tgttctcggc ctcgtaaacc gcagccatgc ctccccgccc aatctcgcgg gtgatccgga
     1861 acttgccttc cagcacggtt ccgatcggga ctttggaggt gttactgatg gccatctacc
     1921 ggggccgcgg cccgctcggc actatattgg gggcggctcc gcctcgaaca gaccagtcgg
     1981 actcgggctg ccgtttaggt ccgcggtctg gaataggggc aggtgaaagt ggtttgatgt
     2041 gggttatcgg gttggcgtca gacttggttg caaggtgcgt tattgccccg gcctgaaagc
     2101 cgtcagcact atagccaatg gggtgttttt ttctcagaaa tcggggtgct tccgatggag
     2161 agtggagttt gccaggtttc gcttcgggca ggagtcattt cgctccgaga gactccgtcc
     2221 ggaaacattg cagaaacgtg tcgcccgtta ctcggcaagc acgccgccgc acaatcggcg
     2281 ctgaatcatc gttgattgac gccacgcccg cccggacacg ggaaatggtg tggggtcaag
     2341 gccccggaga atggagagcg atatgaagaa ggtcgaggcg atcatcaaac cgttcaaatt
     2401 ggacgaggtg aaggatgcac tgtccgaggt gggcatccag ggcatgacgg ttacggaggt
     2461 caaagggttc ggcagaacgg gcggcaagaa agaagtctat cgtggctcgg cctacgtcgt
     2521 ggatttcgtt ccgaaggtga agctcgagat cgtggtgccg gaccaaatgg tccaccaagt
     2581 catcgacgcc atcgagaaga gcgccaagac cggccgcatc ggtgacggta agatcttcgt
     2641 cattccaatc gacgaagccg ttcgtatccg taccggtgag cgcggcgaag aagccatctg
     2701 acattggatt gcgcctcttc gaggtgcgag tcgaggtaaa gtcggcgttg ggctcgggca
     2761 cggctgccta ccggataggc ggctcgcccg aactctactt ccaggctttg ccaggatggc
     2821 agttcgggtt ctggttccgg cgcgcgggga gttactctat gctccgccgc ctgccgggac
     2881 tgccggcgtg cggcgtcgct cgctgagggc tccccaaggg gccggctctt cagcgcgagg
     2941 acgccgccct gcgagaggac ccgggcgtgc aagaaggaaa cccgggcttg gaggaacaga
     3001 tggatcccaa acaagcaatc gagctcggaa agcgcaacgg tgccgtcatg gtcgacctca
     3061 agttcgtcga tctggttggc aaatggcagc acacgtcggt ccctttccag cgcttgaccc
     3121 cggagtcctt cgaagacggg ttcggtttcg acggttcgtc gatccgcggc ttccagccca
     3181 tcaatcagag cgatatgctg ctgattccgg atgcgacgac cgcggtgatt gacccgtttt
     3241 acaagcatcc gacgctgtcg ttcatctgca ccgttcagga tacgattact cgtcagagtt
     3301 atacccgtga cccgcgctac gtggccgcaa aggccgaagc ttacctgaag tcgaccggta
     3361 tcgccgacac cgcgttcttc ggtccagaag cggagttctt catcttcgac gacgtgcgct
     3421 tcgcggccag ccgtcacgaa gcgttctatc gcatcgactc ggccgaggcg gactggaata
     3481 cggggcgcga agagaacccc aacttgggtc acaagatccg gcacaaggaa gggtatttcc
     3541 cggtccctcc tcaagatacc cagcaagatc tgcgtaccga gatctgcctc acgatggcgc
     3601 aggtgggcat cgaggtggag gttagccacc atgaggtggc cactgcgggc caaggtgaga
     3661 tcgacatgcg ctacgacagc ttgctgcgca tgtcggacaa gttgatgtgg ttcaagcaca
     3721 tcgtgaagaa cgtcgcgcac cgcaacggca agaccgctac cttcatgccc aagccgatgt
     3781 tcggggacaa cggcaacggc atgcactgcc accaatcgct atggaaagaa ggcaagccgc
     3841 tgttcgccgg cgacggttac gcgggcctga gcgataccgc gatttactac atcggtggca
     3901 tcctgaagca cgcccacgca ctcgcggcga tcacgaacgg caccaccaac agctaccggc
     3961 gtctcgtgcc cggcttcgaa gcgccggtga acttggcgta ttcgaaccgc aaccgctcgg
     4021 cggcgatccg catctccact tattctccgt ctccgaaatc acggcggctg gagctgcgct
     4081 tcccggatcc gacgtgcaat ccgtacctcg ggttcgtggc catgatgatg gccgggctcg
     4141 acggaatcca aaatcgcatc cacccgggag accctttgga gaaggacatc tacgcgctct
     4201 cccccgagga gctgaaggat gtaccgcgca tgcccgcctc gctcgagcaa gcgctcgacg
     4261 cgctggagca ggatcacgag ttcttgttac gcggtgacgt gttcactcgc gatctgatcg
     4321 agatgtggct cgactacaag cgcgaggcag aagtccaagc ttcacgcttg cgcccgacgc
     4381 cattcgagtt ttacctctat tacgacgtgt gagctcgagt cctacctaat ccgtgcctgt
     4441 gggggcaagc tgcgaggcgc gcgattgtgc gctggacctg agccggtgag cgtctgctag
     4501 tatcccgttt gggcccagga agtccgccag ggcgagcgca ggtacccaga acttgccgga
     4561 cgacgagtat ttagaagcga ccctagaggt cactatctcg cgggacatga gcagcctgat
     4621 tcggtcgact gcaccgagtc aggcgctttt gatcgtgttg tccggtcccc gtctaggcac
     4681 ccgcagcgta cttggtgaga gcgcggtgga tgttggtcgc ggctcgcaat gtcacctgat
     4741 tttggatgca gactcggtga gtcgtcgaca tgcgcgcgtc gagtggaacg gccaagagca
     4801 ccggctggtg gatctcgctt ctaccaacgg aacgtatctc aatggctcgc gcgtgcgcga
     4861 ggccgtactg aaggatggcg atcgcatcgg catcggtaag gcgctgctca agtacgtggc
     4921 gggcggcaac atcgaagccg cgtatcacga agagatccaa cgtctcgcac gcttcgacgc
     4981 attgactggg atctacaaca agcggcagtt cgaggagagc ttgcgcttct cgatgctagc
     5041 cgcgcgcacg gcgcccaagc cggtgagctt gatcgtgttc gatatcgatc acttcaaacg
     5101 catcaatgac actcatggac acacggcggg agatcaggtc ctgtgtgatg tggccaaggt
     5161 cgtcaaagag gggctcggcg agggctatgt gttcgggcga gtgggcggcg aggagttcgc
     5221 catcctgtgt gaaggatcgg gcctggaagc ggcggcggag catgccgaga ccatccgctc
     5281 gcgggtttcg cagcatcgct tcgcgttcga gggcagcgca tttccggtga ccgtcagctt
     5341 gggggtggcg gagcgcaccg ggggcggcga cgaggagccg gagaagctat acgagcgggc
     5401 tgacgagaag ctgtatcgag cgaagacgtc tgggcgtaac cgcgtctgtt tttgagcgag
     5461 cgtcgcctcc aatccgtcgc gcgcgagcgc ccaaatgaga aagagccgcc ccgccgggca
     5521 gctctttcac gcagatcatt acgttagctg aggttcacgc gctcgcgctg gcgcggcctc
     5581 gacgcgactt cgggcccgac ggctttgctc cgcgtcccga agctttgggc gccggggcag
     5641 agggagccgc tttgggcgcg ggagcggcct tggctgcggg agccgcggct ttgctggctg
     5701 aaggaggcgc ggctttgctg gctccgccct tggcgggtga gctcttcgcg ggggtggcct
     5761 tggccggcgg agtggacttg ggtgcagcgg cggccttcgc gccacgtcca gaggcagcct
     5821 tcgcgggcgc cgcgctcttt gctgcgggct tgctcatccc atcttcttgg gtggcagcac
     5881 cgttgctctt ggctttggcc gctttgccct tgctcgcgcc cttgccggcg cgcagcagat
     5941 catcagcgag ctgagccgct cgcgcggtgt tttcccagct gaactcggcg cgcccgaagt
     6001 ggccataagc cgctgtgggg cgatagattg gccgcagcaa atccagctcc tcgatcagcg
     6061 cgcgcgggcg catgtcgaag ttcttcatca cgtacgcggc aagcttctcg tcatcgatct
     6121 tgccggtccc aaaggtgtgg acgtaaacgc ccatcggttg ggccacgccg atcgcgtaag
     6181 ctacctgcac ctcgcagcgt gaagctagac ccgccgcgac gatgttcttg gccacgaagc
     6241 gagcgtagta gcaggcggag cggtccactt tggacgggtc tttcccgctg aaagcaccac
     6301 cgccgtggcg gcccatacca ccgtacgagt ccacgatgat cttgcgaccg gtgaggcccg
     6361 catccccgaa tggccccccg attacgaagc gcccagtcgg gttgatgaag tacttggtct
     6421 tgttgtcgag caagttggct ggcagagctt tcttgacgac cagctccatc acggcttcct
     6481 tgagagcctt gtactttacg tcgggggagt gctgtgtgga caccaccacc gtctcgatgc
     6541 gcgatggctt gccatcgacg tattccatgg tgacttggct cttgccgtcg gggcggagga
     6601 agctcgcctt ttcggctttc cgcaccgcgg cgagcttttg cgtgagcgcg tgtgcgtaca
     6661 tgatcggcgc tggcatgagc tcgggagtct catcgcaggc gtagccgaac atcaaacctt
     6721 gatcgcccgc gccttgctcc ttgaagagcc cttcgccttc cgtgacgcct tgggaaatgt
     6781 ccgccgactg cggttcgatc gcggtcaaga taccgcaggt ctcgtaatcg aagcccatgg
     6841 atgaatcggt gtaaccgatg tccttgatga cgccgcgagc gagacgcccg aagtcgatcg
     6901 aggcttgcgt ggtaatttca ccggccacga cgacgaagcc cgtcttgcac agcgtctcgc
     6961 aagccacgcg gctcttcttg tctcccgcga gagcggcgtc gagcacggcg tcggaaatgg
     7021 cgtcgcagat cttgtctgga tggccttcgg taacggattc ggacgtgaag aggtagcggc
     7081 tcatctgtgc tccgtaaagc ggctccggga ttgtttgcgg ggctgcgtgg tttagtgcga
     7141 gctgtagaga ttacggactc gaacgagagt gaaacgaggg tttgcaggcc ttgcgcagcg
     7201 ttgcgaatgc ccgccgaggg tcgtcagtcg cgttccgtat tggagcttga ccccgaccat
     7261 ggcggcgcaa actagcacga atcggccgct ccgaaagccc catcggtggg gctggaccgg
     7321 ccgagttcgc gggcggggag tcagtcgggt cttgttgggc gggctcgcag ggcgggggcg
     7381 ggtgttctcg cgacgcggac gcgaatgacc tggtccttgc gaatgagagt caattttacg
     7441 ggcgccccga cttccccaga gagcgcctgg tgcacttcgc gaggcgacat cgagcgtgga
     7501 tcacgcccat caatcagcaa gacttcgtcc ccctcctgca ctccggcctg ggcggctccg
     7561 agccccgacg ggacttcctt cacgaccaaa cgttgattgg gctgctgcac gagcatggcg
     7621 ccgatcgttc cgcgttcgct ggtgcacgcg agtccgcccc ccgagcaagt cacgatgcac
     7681 aggagcatga atcggcacaa agaactccca ggcgcgggcc cagctagatc ggtcgtatgg
     7741 ccaactctgg acccactcct tgggcgattg gagcgctgcg gtttcgccat cgcgcgcccg
     7801 ttgtacgcga atcgcgcgcc tcgatccacc aagccagccc gggcgaaccg ctattcgcgc
     7861 ctcggcgttt gcttattgcg cggcttccgg ctgaagggcg cccgccgctg cctcggcacg
     7921 cgggcgctca ccatcggtcg cggtggtgag cgatgtttca ccggtatgcg cgttggtcag
     7981 ggcgcgctgt tccagagtca acgttacgag gttcgacaga aaatacgcga aggtctcctc
     8041 gtcttcagtc cagcgccggg ttccgcccaa gtgttcatgg caaagcactc ccaccatgcc
     8101 tcccgaagcc caaattggca cgtcgagcat ggccccgatt ccgaggggcg tcaggtaaac
     8161 cgcggaaaag caggctgtcc tcgggtcggt gtgcgcgtca tgcgcggcga tcgtgcgctc
     8221 ggaaaccaaa gactgaaagt agctcgggaa gtcagcggcg ttcagccgcg ttccagcgct
     8281 atgatgagcc ggcgtgcgct cgaacagatc ggcgcatacc agcgtgcgct cgtgcaagct
     8341 ttgactcagc ggcgcgtcca agagccagac actcacgcgc tcgattccga gcgtggacgc
     8401 ggcagactcc gtgattcggc gcaccgtcgc ttctagattc ccggcgaaca gacctcgctg
     8461 cgaaaacagg tccaacaata cgttcaaatg cccgcgcagc atgtcgaggg tggccggcgt
     8521 tggtgtacgc accgctcccg cgaggatggc agctggtttg tccggcacca tcatgaactc
     8581 actgcggacc aggttgagcg cggtttcgag cttctgtatc aactcgggag agtctccgcg
     8641 cgtgctcgct tcggccgaag tgacctgtga caacgaggcc aggcgcatct tggcaaccaa
     8701 gcctccgagt tgattggctt cttcgaagta gcgttgtccg cggataccca tagttcgccg
     8761 tttccgcgcc tctagagggc gccgagacgc agcaacgtcc gccccggacc cgcggttaac
     8821 ttcgattggg caacggcagg ctcttccgca tgttgcccgt cacggcaact tcacgctcca
     8881 ccacactgca atcagccagc ctacacccgt aacgagcagc gccggagaag ccagcaacaa
     8941 gtctaccggg gaaccgccgg tctttcgcac gtgcacgagg cgcatgtagt ccaacacccc
     9001 gaacagcacg aagggcagcg aagcgaactt tcgcgtctga atcagaacgt tcgattcgag
     9061 cgaatacaac gcatacccca ccaccgccat cgccgccgta atggtcatgg cgtggtccaa
     9121 gaagctctga ttgtagccca gcaacgacgg gcgatgggag ctgtccagcc ccttggtgac
     9181 atccgcgcgg cgcttcgcga gcgccagaaa caacgccagt gcgtacgagc acagcaacat
     9241 gggatgcgaa ggcggcacgt ccaaaagcgc gcagcccaag agcacccgca gcacgaagcc
     9301 ggaggccagc aagaatacat ccagcagcgc gatgttcttt gcgccgaggc aatacgcggt
     9361 attgatggtg aggtacacca gcagaatcgc cagcgcgggc atactggcgg aagcgaacgc
     9421 caacccgatc gacgccacga cgagcgccaa ggaccacagc ttggcttgca gcagagagac
     9481 acggcccgaa gccaccggcc gcttgcgctt tttctcgtgc gcacgatccc gctccgcatc
     9541 gagcgcgtcg ttgaacgcat atactgccga gctgcccaag caaaacgccg tcaaacccag
     9601 cccgaacacc gcaggcttca aggctgcgcc gctcgccaaa gcgaacggca ccggcatgaa
     9661 cacgaagacg ttcttaatcc agtccttggg gcgggaaagc tcgatgtagt cgcgaagcat
     9721 gccttcggac cacgccttac tcgatcttgg ctcgcgactc cgcaagggag tgcgtgcggg
     9781 ctgtgatttt ttttgatgtg ctcgaggctc gcgcctgggg gcaagccagg aagcccggaa
     9841 gcgagtgctt cgctccgtcc cctggcgtgc cggtcgtccc gccggtactg gatgccgcaa
     9901 cctgcgcagc cgtcacggcg attctatcga ggatgctacc gcctctcacc gcttcggcgg
     9961 cctcgcacgc ggtcggcctt cccgagatgc tgatcctaag gggcgagtcg ctcgtaaacg
    10021 gatcaatcac cgtctcgaac ggctctatcg ctcagaccgg ctcggctgaa ctgaccggct
    10081 cggccccaga ttacccctct cacttccgtt cactcgtcct gcgttcagtc gcgcctgcaa
    10141 acccaacggc gcctttgaat acaggtcccc ggactaacga tgttctaccc tgcgcctata
    10201 gccgctcaga ccgcgacctg ttccactgcg aaactcggag tggcctcgga tgtgccgtgc
    10261 tcgcgcgcac ggcgcagggg cggactgcaa tccgggcaat accaaacggc gcaagacccc
    10321 tccgcggaac gcactatcca gccctgttcg ggcatgagca gtgtgaagcc ccacaccgtg
    10381 ggcggcgata gcttattgca gctcgcgcaa cgtcttggtt ccagggtccc gctcggtgtc
    10441 attcgctttt cctcctctag ctcgttcagg cttgccgtct ctgtcgctcg cgccgctcga
    10501 gttgcgatta ccggcttcgc gagtcgttcg tcttggtgtt caggtcgcgc tggggggcag
    10561 gttcgatgct gcgcccgctt ttcaaagctc cgactacttc caaagctccg ctcgcgaaca
    10621 gagccttgcc ctgggaggcg tcatgctctg gatcgcccgc tctgctgggg gcggttcgcc
    10681 tctctccgcc gcgggaagcg ctccgacgca gtatcccgct tcggtccgtc cctcgtttcg
    10741 tcgctcgatc cgatccctca gctgcaaaaa cacgcggctc gtgccgcgaa gcacaaagct
    10801 cacgaacccg attcgaccca atcgaccgcg ccgaatcggc cctctcaagt gactaagcct
    10861 cagtcagtca aggcctgcat caatcgaggc ttccctcaat cgagcctagt ttcgcagagt
    10921 acgttgggtt tacgcgccgt caagggtcgt gatccgacgc agcagtttcc ggccaaaatt
    10981 aacataattt gagttaggct tacatcggct ctgctcgcgg cgtggtgggc gggctaaact
    11041 cacgagaagg cttccgcgca tgactcaagt cgatcaagaa ctcccggatg gttgggccga
    11101 ggctttgcca gacgaccctg ctttcgccaa gttcttggcc gagcaattgc ccgaattggc
    11161 cgctgatgcg tccgggcatg cccggactga ttcagcacag gctgaacgag agtccgcgcg
    11221 agccggcgga gcttcagccg ggcaggcttc catccgcggc gatgtcacgg ctccgaccgc
    11281 gggagtgaac cggctgctcg cgtcggtcgc tgaattacct gctcgttatg cgcccttctt
    11341 cggccgaatc gccaccctgt gggatgtgtc ggaggaggtg gttacggcgg tgttggatcg
    11401 cgcgcgtctg cagtccgcgt ggaagcgcac gccgctgccg ggcgtacggg tactcgaagc
    11461 ctcgggaggg ccacgcacat ccggcggtga tgtcttcttg gttcggtttg ctccgggcac
    11521 gcgctttcct cgccaccgtc accccgggcg cgagctgatg ctggtgctcg aggggagtta
    11581 caccgatacg ctggggacgc gcgttgccgc gggcgacctg cacgatatgg ggcccggctc
    11641 agaacatggc tttctcgtca gcgaaaatga gccgtgcatc ggagcggtgc tgcacttcgg
    11701 gcgcgagttc accaatccgg ttttgcgggt catcgacaag ttgctgggcc gctgagccca
    11761 gccccaggcc gccctcatag acctggttga tggtcgtcca cgcgtcgcgt gaacgatggg
    11821 gcggcctgcc gcgaacgatg tttcatagct ccaattcaga gctcttagag ctctgcgagc
    11881 ttacccgtgg tcagcttcgt atctggctgg gttccgtagg actcgatttg cacgccgaag
    11941 gcttgcgcga tggataccag tagccggttg ttcggtacgg tttggtagtc ggggccgttc
    12001 atgtcgcacc aggtttccat cggacaatcc gtgcggtact tgaggcgccg acccatgcgg
    12061 atcttcccgt tggctccgcc cgccaacaac gtcggggcgt tgcgagtact gtgccccgcc
    12121 gggttgccca tgtcgctcga ggcgtaaatc agcgtgctat cgagcacgcc gagctgattc
    12181 agcttctgca tgagccgcgc caccttcgaa tagctgtagc ggttgaactt cgcgagcgtt
    12241 gcccacgtgg aggggttacc ggctccgacc gggttgccgc tgctgccaat cgccgaagaa
    12301 gaataggtat gagctacccc accgtgattg tcttcgggca aaccgagagg attattcgcg
    12361 tacgataggt cgttcagcac cagcgtcccg aagcgggtga tgtcgcaagc catggcttgc
    12421 gccagaatgt cgacgtgcgc atcggtaatg gcgtcgaagt acggctcacc cccgttgtag
    12481 cgttcgagct tggggaaggc gttcgggcga gctagcgggc tgcacgagcc gctgcctccg
    12541 ctaccagaag tgggcgctgc gaactgcttt tcgagttcac gcaacgaatc caagtgttgg
    12601 tcgagctttt gctgttcgtt gggcgcgagc cgtgctcgca gacgcttcac gtcgccattg
    12661 acgtaatcca ccaggctttg ccccaacttg cgttgccgtt cggcggccgc ctgagccgct
    12721 ggatccgtgc tcgtgaccgc tcccttgaac agcaaatcgt acgcggccac cggatcgatg
    12781 accttggaga tgggcgcgcc tccgggaccg aacgacagcg tctcacccgc cttcagctcc
    12841 ttgtttccga ccgcgagcgc gatgctcgtg acgcgggtgt tcttcccgag tcccttttcg
    12901 accgcgagaa actggtcgag tgaactgttc tgcggcttct gtccgccgtc gatgcggctg
    12961 ccggttagga tcgtgccggc cgtgtcgtga ccgttggcat tcgatagcag atcgatgcct
    13021 tcgatcacca cgatcttgct cttgaagctt gcgccgtaag tggccgcgtc gtcgaaggga
    13081 gcgagcggac tattttcgta cttcaggttg aagttggtct cggtgtccga agcccgcatc
    13141 gtgaatagct cggccgcgat gccgtgcggg tgataaacgc cgatgaactt gagcggcagc
    13201 gcttcaccga ctgattgagc aaatgaattt tcgaccaagc gatagaacgg caaggtcgca
    13261 aacccggccc ccaccgctct caagaaggta cgccgactgg ggcttaggct catggcattc
    13321 tccgctgcgt aaacaagggg ctcttcacgt aagtaatcaa ggtctcgacg atgccgccgg
    13381 ccgaagccac ggcgtttccg cgccagttct cgatgaaggc attctccgtt ggcttcagcg
    13441 cggcgtcgtt ggaagcggag gcagcccgga atacgttccg tgcgaagcag tcacgcacct
    13501 gggcgctgtt agcgagcgct ttggcgagct cgttgctgtt ggcgtagctg ccgtcgaagt
    13561 cggtgctcaa gctgatttgg acggcgctgt tgacgggttt gttgttctcc gtggaccgga
    13621 acgcgcccat cccgtcgaat tgctcgaagg aaaatcccag cggatcgagc acgcggtggc
    13681 aagacttgca ggcatcgtcc gtggagtgca cgtcgaagcg ctcacgggtg gtcttcgccg
    13741 gatccggcac cggcggcacc acttggatgt tcaagctggt gggagaggcg atcgatatgc
    13801 aggccacccg ccgagccagc gccacaccgc gcaacaccgg gtgactctcg tgtgcgttcg
    13861 catacaccga gaggaacgcg gcttgattca ggatcccgag gcgatcggtg actttgctgc
    13921 tattcgcgat cggccctgcc ccctgagttt tgtagatgcc gagcggcgcg ctcgacacgg
    13981 tccagtccgc gcccaagagc tccgagagct tgcctgagga gtcgaacatc acacggcgtg
    14041 ggaagtccga gctctcggct acgatggcgg ctttgctgct ctcccaattc ttgtagacga
    14101 ctgcgtcctt cgaggtctcc gaaatgcgat cgagccctag ccactcgcgc accaggcgca
    14161 ccgcgcgttc ctgcgcgccc gcatcttcac cgagcaagcg gcgcgcttct tcttcacgaa
    14221 cggccgggtc gtcgagcgtg ccttgcgagg ccttgtccag cagcttctga tcgggcggcg
    14281 cggcggcaat cagatacgat atcgaattgg caatctcgaa ttgcgtgagg gtcagcgcgc
    14341 cgccgctact gccgccgttg ccgagctccg tgagatacag aaagctcgcc gactgaagca
    14401 tgcccttcag cacgtgctcg acgccgtcct ggtaagtggc accgtcagcg gccacccgat
    14461 acagggccaa gagagcgtcc gtttcctcgt tggccaacgc gcgccgatgc acccgcgccc
    14521 cgaagctcga gatgaaggtg cgagcacaag cctcgccttg agcggtgttt tggcaggggg
    14581 cgagttgggt cagggttccg ttcttgcgcg cctctgcggc cagcgcttcg gcgctcgccg
    14641 aaaggctgcg caccatgacc gcgtccacgc gctgtgcgtc gttcaccgtg aagccatttt
    14701 ggcggagatc aggcgggaac tccgcggtaa gcgctttggc gttcgcaccg agcagcttct
    14761 tcacggtgat atcgtattca gcgttactga gccgccgtat tctggccggg atgagcgcgg
    14821 aggtggggac ggtcccgttg ctttgagtgc ttccgccact cgataccgcc ccgccttggc
    14881 ccgcattctg ccctccgctt tgtggagtgg agcctcccgc gttgatggcg ccgccattgg
    14941 agggtgctga gccggttgct ggccctgagc ctgcagtctg attaccagcg ctcgcaccca
    15001 tgcccgagcg accgcccgtg ccgtttccga ctccacccgc cccagcgctg ggaatggggg
    15061 aagtgccagc ggcgcctccc aaccccagcc cgctcggatc gctactgcag gctaaaacta
    15121 gcccgcacga gccgatcccc aaacgggaca accaacggtt cgatatgcgc attaaactct
    15181 tcctcttact tgccaacgct tgccagacgc ctgcgaacgc cttttggcca ctggcgtcgc
    15241 cagccttgct ggctggtcag tggcgattcc gcacatgacg ctgggacgag aagtgtacgg
    15301 gccgcaagca atgtaaaaca acgttctttg atgccaaaac tctcgtgggt gtagcctcga
    15361 tgaagttctt cgaaacgcga aagttccagt aattggcatg ccgcaaaccg ctttgattgt
    15421 ccggggtgtt cgacccgatg gtgcgcgaaa gacggccatt ctcgttctgg gctgtcgcga
    15481 cgagcctcgt gaacggtgtt gatcacgtga acggtgttga tcaatcgcca agttatcaat
    15541 cgccaagtta tcaatcttcc aagcgccgtg ggcgctcgac cgggggcgag tgtcacaacc
    15601 ctcgttgcaa tcgagccgcc agcgctccga ctcgatccac tgcgattcgt gggatctagc
    15661 cttaagggag ccagacccaa aggccggcag cggcggccgc gccagctctc tttcgcggcc
    15721 agggatttgc ggtttgagct cttcaatctt tcggtctgtt taaggtgtcg gactccggct
    15781 gggatggctc actattttgc tgtctagccg ccaatcacgg tgcctaaggt ctcgcccacg
    15841 gtgtccggcg cactactggc atggagtcta gctttcgcct gacgggggcg aatcttggtt
    15901 ggatgggatt cccgtgtggc gcgcctacgc ccatgtggag ggccgcccaa ttagcgaacg
    15961 ctaagacgcc cagtgagctc tgacgacttg gacgagctcc gatgactcta acgagctctg
    16021 acgactgcgc cgactcttag ccgtctagac cgacttcgat gcaactcgag tggcaccaaa
    16081 ttcgttggga gtctgcctcg agagacgtcg aatcacaacc caaaaggttg aacagctaga
    16141 tttccgtgag tggagcagtt ctactgctca gtgagtgggt gctcgcgtcg cattgccaat
    16201 tgctaatcca aagccctgcg cgctgggcga atgcgaatgc tccgggcgca gatcaggtgg
    16261 ctgtgtgacg gctcgcgagc agcctgtatt tcccgattcg gctcccccac tcaccgacct
    16321 ccacacttcg cacgttgcaa gcctggcgac aaccgcgttg cagtcaaagg ctcgcgtccc
    16381 agctcgactc ggcacactgc gcgctggacg tgcaccttgg tccccgactt cggggcaaag
    16441 cagacccagt ttgggctcgt tccagcagac taatgccagc ttgcgccaag ctgaaggccg
    16501 gcccgacgtc tgcggccggg cttcgagacc ggcggccgcg aacaccgtcc ccacgttagc
    16561 gttctaaaat cgtcaagcat ccggtcaggg atcgctccgg agggtttgct gatgccgaag
    16621 gtctattata gtgggacctc atgcgcgtct cgtggctggc tccggctgct gcttccgcag
    16681 ccctgttcgc gggagggtgg gtgcaggccc gcacgaccac taaacccggc caggcggagc
    16741 ctgcgtctcg tggtacaccc acttggtcca atcccgagcc gaagcccgag ccgaagaagg
    16801 cagagcttaa gccgaaggca gagcttaagc cgaaggcaga gcccaagccc gttcgccgct
    16861 ccacggagaa gaagccggaa gccaagtcgg aggcgaagag cgtcgtcatc gagtgctacc
    16921 cggcctgtcg agacggcttc gtctgcgcgc gcggggcttg cgtttcgctc tgcaatccac
    16981 cctgcgcttc ggatcaaatt tgtgtggacg agggcaagcg ttgcgaacag ctgccaactg
    17041 caacatccca gagcttaccc tcgccgagct cgtcatcgag tagcgcggag actccgccgg
    17101 cgcccgcggc gacagcaacg gagcccgccg ccgcgcccgt ggaaccccca attccggagc
    17161 ccgccccgcc gcgacgcaaa ccatactacg agcgcagcta ttccgggctt ggttttcact
    17221 tgggtctggg cggctcggtc gatcagagcg gttcgaagta cactttggac tcgacgctgg
    17281 gtttcaacat ccggtcggat atcccgatct cgaagtactt cgtaattggc cccctattcc
    17341 agttcgcggc ttggcgaccg caggcggtgg gactgtcact gagccgcagt tattacgtag
    17401 atttggacct ctacctacgc gcgcgcatcc ccgtagagtc cgaatcacta cctatcgagt
    17461 tttggttggg agcgcccgtc ggcgtgagtc tcaacttctt gtccgatcca cgccccgccg
    17521 tccaaaatac ctttagcgtg ggttggaact ttggggggct gattgggact gcgctgcatc
    17581 tcaggcaggg cttcggggtg ttcttggagg gaggggccgc gcaacaccgc accagctacg
    17641 acggcgcgca gggcgcgcgc gtgtccctga ctatctcgca gttcaacttc aacgctggct
    17701 ttaccttccg caactgagct gcccgccgct ggagccttgc ccacaaaagc cgaatggctc
    17761 gcgggtcacg acaaggcgtg acgggcgagc cacctggctt cggtcttcat tgggccggcg
    17821 ggcagccctc gctccgacta actcagtagg ggcactggat cgtgcccttg gccgcttgcg
    17881 cgagctgttc gcgaattttg ggctccacga tcgtcttcgc cacctgattg accgcctctt
    17941 gagcgcccgg cagattgcag ttctgcatct ggaccgtgta gttcatgacc agattctcgg
    18001 ccgcaatgct ggctgcctcg ggggtcttct gaatgaccac gtccgccgag taggtcagat
    18061 tgttgatcac ggcggtgaag ttgcaggacc cagaaatcag accgagtgaa acgctgagcc
    18121 cgcccgtgac ggtcaggttg gccgtccccg acatcttgct ggttgcagaa tcgtaggccg
    18181 ccgtgaatcc ggtggcgact gcgtcgcaac ccacggcttg gccctgacac gccgcagttc
    18241 cgcacaggtc gacgcccgcc acgctcgtat cgcgtggaat gcagctgtgt acgtcgttca
    18301 cggccttggc gctctcaccc aacatggcgt tgatgcgttg tccgagccac tcgtccgtac
    18361 agcacatctc gccgtcgccg agcacgccgt caccgcattg attgccgggt accgaggtcg
    18421 taccgcctga gctgtcgctg ccgccactgc tgctgatgcc acccgtctca ctgctgccac
    18481 cggcggctgt ggttccgccg gtgggtgtgc cggtgcccac cgcgccgccg ctgctgccgg
    18541 gcgtgccgcc ggcgttcacg gatccacctg tcgtggccgt tgtcgctccc gtgccggtaa
    18601 ccgctcctgc acctgtggcg ccgcccgtgg cggtcgcgtt accggagccc gctgttgctg
    18661 caccacccgt cgccgaggag gttccaccgc cgcaacccgt tgctgttccg cccgtcgccg
    18721 gatccgttcc gaacgggtct tcgccgatcg gccgcaagca gcccgcgctc gcaaacgagg
    18781 tcagcgatcc cagcaacagg ccacacagtt ggatgttttt gcgcatttgc ttcgattccc
    18841 tcctgggcac agaacgcccg ggctagttct ttctatcgtt tggttgaagg ttccgttgac
    18901 tgcggactcg ccggcggcgt gggccccgca cccatgaagc gactagaaac gccgatccaa
    18961 gtaatgtcgt gagtcgattc gttcgactcg atcgatcaga gcgcggcggg acgaaccgac
    19021 aacccgcggc ctgaccgccc gtgaccgaag gagaccgagc gggcgctgag gtggacccat
    19081 cgacgctcgg gttctgagga agcggctcgc tcgacgcatg ttcgcgggcg aaatcttcga
    19141 agctctctgc gcagagctcc tcgttattcg cgtcccctgg gcaaaccaag ggttgagtga
    19201 cttgcgagaa cagcagccac gggcgcagtg tgagctcgat ctgagctcct gacatcgctg
    19261 atgcctctgg cagctgacgt gcgtcacccg ttggtgcgac gaatacgccc gcttgattgc
    19321 cccgagcgcc gcacacgaac aaggcgcctt cgtggacggc gagacaactt acgtcagtca
    19381 cgtccgttgt gacggcctcg aacgaggcac cgagcgcgtt ggcccggaac aaccccagcg
    19441 gattgcccag ccaatacacg gtatgatttc cgtcggcggc gctggcaccc gccgtttcgt
    19501 tgccatcggc gacgccattg gtattggcgg tgccgagaac cagagtgccg agcccgcgga
    19561 atacttccgt gaaactgcgc cccgcgtccc tagtcagcag caggcgttcg tcggaatctt
    19621 gattggctcc ctgagtggcc acgaacaccg agttcggatc atcggggctc acggccaaaa
    19681 cgcgggcgcg gatctcgctc gcctcgagtg ggatcacgtg cgccgtaaaa gactgaccgg
    19741 catcatccga agtgagcaac tccgcctgac tcgcggcggg atcttcgagg ttcattgcgc
    19801 tcgcgtacag acgctcgggc ttcgacgcgg agctcgcgag ccccgtcacg tagtctcgaa
    19861 tgtcactgcc cctcgtgaat gtcagcccgg cgtcccggct agcgagcaag cgccccggtt
    19921 cggcctccga ggcggaggtg acgacataaa gagtttcgcc gaactgctcc atgacgacga
    19981 taggagcgcg gccgatctcc agcgtgttct tggaggcttc ggcgccggtc gaggacacac
    20041 aaccggaaga atcgatatga cgcagcccac caccataact tgcgatccaa accccctctg
    20101 acgtaaccgc ccagggtacg ttttcggaca acgtggcgcc gtaagcttca ttgcagagca
    20161 aggcaggggt ggtgactgcc caagcggctg cgctcgtatt gccctgcatg gcgccgccct
    20221 ggatcgtgtt gctctggatc acgctcccct ggatcacacc gcgattggtg cgtaaccaca
    20281 cgatcgatcc gggctccccg ccagcctccg cggctgacgg ctcatctgcg tgcacccagc
    20341 cgatcgcggt ggcccgcgcc ggagcgtgcg cgaacgctgg cgacgtcgct agggcagacg
    20401 tgattgagca ggcgagacac atcggaacaa ctcggagaag agccactaga aaccggcgca
    20461 gttgtacttt ctgaacagtt gaaaatccgg agccagccca cctgccgggt aagcaatgac
    20521 gaagttgtaa cacatctcct gattcgttcc ggcgccaaag ggcgttggca ttgcgtaatc
    20581 gcaggtggtg gagagcgtat ctcccgggtt cacgattgcg ggggtcgaat acgtgacttg
    20641 agaattgaaa tcgaacggca catcgagcag cgtatcggtc gtgccatcta cgcgatgaat
    20701 gatcgtggac atgtggcgcc cctgcagatg catgtgcgga gtcgccgaaa tgacggtgac
    20761 gggaactcgc gtcgacggtt cacaggtgcc cgtcgcagac aacttattca gattctgtgt
    20821 gccgagccag tgtacggcgg cttcgtgctt tcgcagcttg gaagtgacgc agatttccgc
    20881 cccgctcgcg tcgggctgaa gcgtccctgt tccgttgctg tagtgcattt ccagcgtgaa
    20941 cccgcccggc tcgatgcgca gcccaacgtc cggcggcata tggagctctt cgccaccagg
    21001 ggcccagccc gcgatgttcg cagcatcggg gtgtgcgcca ccacagctgg tgtgagcgcc
    21061 atccacgact gcatcggtgt tgttgtatag aatccagtgg tgaagaaccg cggcgttatc
    21121 gatgatgggc cgtgcactca cgagttgaac tttctcgtcg ccccacggtc ggtcgtattc
    21181 gaagcactga taatagtctt tattgagcgg gaccgggtac ttctctcccg tcgaagagta
    21241 acgcgccacg atcgagtaac attttacgtc ttcgtccgga tcggtcgcgc cactgctcgg
    21301 tacgagcgga tccgtggccg cggtcggggt ctcgcagtgc cctgccggcg ctcccgaatt
    21361 caaccaactc tcgagcaccg ccaagtctgc atccgaaatc cgcgcgttgg gagccggtgg
    21421 cattggcctt acggcatcac gcactcgact caacacctga ctcgaggcct cgccctggaa
    21481 gtccgccgcg gaaatcagcg ggtgtgtcgc gccgaagcgt gggctcgagg cgtgacaacc
    21541 ttggcaattg cgagccacga tggcattgat ttcgcacggt aagtccgaag cgagatgact
    21601 cgaagttcca ctggttccac tcgagccgcc cgcggcgtcg accggcggtg cgccggagct
    21661 cccgctgctc gaattggagt tgccagcgac ccctgcgccc ggagaactgg gcactgcgga
    21721 gcatgcgcag gcggaatagg cactgccgtc ctgcgtgcaa gcggcggtgc cggtggcccc
    21781 gctgctgcag gtgcaggcaa cgctgctgcc gggttgacag acgaggggct gaccactcgg
    21841 gtcactgctg agggggtcac tgccggtggg tgagcacgag agcgcgaagg ggaccgcaat
    21901 cgtggctgca aactcgcaaa cccactgcac gcggtgccgc ttggaatcac gtacttttcg
    21961 gcgtgtcgga acctggcctg tcacgattca tcctctcggc acgggctgct tggacggcgt
    22021 cgttcagctg acgctgatgc ttaagttcat tcgaaatgag ctcgagctcg ggcgctgttt
    22081 ttcgaaccta gattccgctc cgcaggtttg acgccgcttc cgcggcgcga ggaacggaac
    22141 ctaggtcgca tccgaaccga gcgttacggg ttggcgcccg ctgcaatcca gtccgtaatg
    22201 atcttgcgtt ctgcctcgct caagggggtt ccgagcggca tcggcaaacg gcaaccgccc
    22261 gacggattga tgacctgggc gaggccgctc ttcgcgacgt ctcccttgac gacgattacc
    22321 aggttttggc aaccgctcac tgccttgccc gacgtcagcg tttggtatgc gctggcctcg
    22381 gtaaacgaga agccacccgc tgggttcgac tcgccgtggc agccattgaa cgcacaggac
    22441 ttctgtagga ttgcgttgac ctgtgcgaac gtcggtccgc tgctggttgc gccgccgcta
    22501 cctgtcgagc ctcccgacgc cgttgcgcca cctgaattca tgacgccgcc cgagctggcg
    22561 ccgcccgacg cggtgactcc accgctggaa ccggttgcac cgcccgagcc gggacgacca
    22621 ccaccgccgg tccggccgcc gaagcctccg ccacgaccgc cgctgctggc gccgccgggg
    22681 cccattccac cgggccccat tccaccgggc cccatgcctc cgccgggtcc cacgccgcca
    22741 ccagagccgg ttgagccgcc cttggacgtg gcgccaccgc tcgagcttcc gcctgagccg
    22801 gtcgtcgcgc cacccgagcc cgtgctcgct ccgctggagg agccgcccgt gacgccagtg
    22861 ctcgtcgagc cggccttacc ggtcgagccg ccactgttct gggtgacacc gcccgttgcc
    22921 tgtgcgcctc cagaggtcga actcttcccg cccgtgctcg ctccgccgga tgcgccaccc
    22981 gttcctgggg tcaagccgat gccacccaga tcgatgggca aaccaccttc acccgagcaa
    23041 gccgtgacga ggcccagtcc catgatgacc gcgagcccaa cgagttccgt tcttcttgtt
    23101 ctcattcctt acctccaaag gggagcaggc tagttaggcc cgctccgtgt tcgatctgct
    23161 tggcgtgttc tcgctctgct tgattgagcg cgcagtcggc gttggcatca cccgtactct
    23221 cgccggcctt ggctccggga gtcgacccga gctggcgccc gtgctttggg gtcaccgcgc
    23281 tttgggcgcc atgcagcgcg cgcccgagcc tcacgagctc tctaatcaag aaatcttctc
    23341 agactaagat cttctcagat taagcgggcg tccgctgatc cggattgtgg agccggaaac
    23401 cggttccaga ttcaaccgag tacgctggag ctcgagctcg tcggatccgt agtcaaaaca
    23461 ccattcctgc tggtcaaaac actattggcg cttgcccccg tggatcgttt cgagaccaaa
    23521 gatcccgctg ccgagcgggc agacaccacc catcgtgcgt gaggggtgca ccgttctagt
    23581 cgggcgcgtt cgggctgacc agagaaatat gaactcttgg ccgcgctcga gaataatccg
    23641 acggaattac aacatccgtg cgcaatcagt agtgccgaaa ctggccaaaa atcaggcaaa
    23701 aaaccgattc caggtccgcc tttttcgagc cctggacgaa gtgtccacac tcggggccac
    23761 aacgtctggc ggagtacctc ggggccgcgc gccccgtgcg aatctgcagc tgagttgcga
    23821 ctgccgctca ggtgctcggt cgtgcgtatc gggcacgtga gttcgccaaa tcgcccggca
    23881 acttgctgtg atttgcagcc cgcgttcccg cgcccggagg tcgaacgttt cggctgaagc
    23941 acggcagttc ggcctagctc actcgatacg cagtgacgct gacgcagctg cgaagctcgg
    24001 agaaatgccc gccgctcccc ctctgacgcg taccccactg ggcaacatcg ccctacgcgg
    24061 gcagcgccgg gcccgaccac gaacctcttt ggggatgcac cctctttggt catagggcag
    24121 ctcgtcacag tgcgtgcggc tacaccgccg cgcgccactg ggctgccggt tggtgcagga
    24181 gggagaaaga tccaggaacg catgacgaga atgaatgcct gttgggtgcg gcccgggtgc
    24241 ttcaccgggt agttgggccc gtcgattcgg accaaagaac gagcctcgag cgaggccttt
    24301 gcagtttcat ggccggtttc ctgcggtacg gaacctcgga tcacgatcgc aaggaaatgg
    24361 cagaactgcc gcgtaactcc gagttaaggg gattccctaa gcctgcattc gacacgatct
    24421 cttcgaaggc gcgttaggag tgaactgtgg aacatgctcg ttgcgtcgct gccaccggcg
    24481 cgttccccac gctaagaggg catcctgagc cgacccagga cgccgagttc ggtcgcacca
    24541 tgaacgctcg acgtacggat gtcaatcatc aagtccgagg atgcgtcatg aagtgacatc
    24601 ctgcgtccga gcaagcgcgt tttcgccgca atacccgcag gtgattgccc acgattcgga
    24661 gtcgcaacgc aattgccgat gcttcaatca ggcgcttgcg aagggcgggt tacgggcacg
    24721 ctctacgcaa aaatcatcgg aatttgccgg ttcacgcgga gcgtgaagtt tgatctaccc
    24781 gtgagagtga ttggggcgtt gcccgcccca tggctcgagg accagcatcg aattcgatat
    24841 gagcgctgtt ttcttgagcc attcgggcct gatgactaga gccatcagcg gggggatcga
    24901 cgattgtgtt cgtgccaaga gcatgaccag cggcattccc gtgcccggca aaacccgccg
    24961 cgctcgagca ggatgggcgc ggttcgcggg ctgcggcctc gacccaatga acactggatc
    25021 tgcgaagaat tgtaccaatt cgctccggac ctggggcagg ggagtcaccg cgcggctctt
    25081 cgactgccag tcgcgcgtcg aatcgaagct gactctgccc cgcgcgatgt taggctcagc
    25141 cccgtgttca ttcgcgcgct cggtagggcc gtgtccccgc aggtaactct ggccttatcc
    25201 gtgctcgggc tcgccggttc gggctgcgtg gagttgccgg agccgccgcc tgaagggcgt
    25261 gacctctcgg ctcgctatcg agaatacgac cacccgacgg gagaagtccc agactctgca
    25321 cgcgggcagc tgtttcagag tgcgttgatt ctcgcgactt ccctgcgcgc cttcagcggt
    25381 ctggtcgttg ttcgctcgct gatctcggac tccagcgtag ccggagcggg ttacgcggta
    25441 gaggggtcgc tggtgcaagg ctcgctcaag gcatccgtgg attgcgcgaa ggatcccatg
    25501 catccggaga tagctggcgg gcacgtcgag ctgctcctcg gaattcgtgg atcgcgcatc
    25561 agccgcgcct tgaatggggc cgtggtcgat tgtcgactgt tggtcacggc cacggacgtg
    25621 gcgcaagtaa actcggattt gaccatcgat ttgggggcag agttggagct tggagaggtg
    25681 ctgcggtcgc ctattttggt gcgcgccgag aacgtagatc tgggtggtga gaccagcggg
    25741 gttcacgacg taaatatcga gtttagaatc caaggctatg acgttctcga gtccctggtc
    25801 gatatgcaga ttatcggtat tccgggcacc ggcagcgtgg tcgttcagta ccgccgagat
    25861 ggatggttcg gggtcgtcga aaaacgcggg atttggacgt gcgcaggtac gggggagcca
    25921 gcgtgcgtct tgaagcagta g
//
LOCUS       DASTKU010000539         5013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1444317, whole genome shotgun sequence.
ACCESSION   DASTKU010000539 DASTKU010000000
VERSION     DASTKU010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444317"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            566..1552
                     /locus_tag="VFQ61_18815"
     CDS             566..1552
                     /locus_tag="VFQ61_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956565.1"
                     /translation="MHFFASRLKYSRWVEVTLVPNQQVETLARTLVDHFAALGGIPLL
                     AVFDRPKTVALSWKQNGEVIEWNPIFAGVVLDLGLGVELCWPHAPQQKGAVENLVGWV
                     KGSFFKQRRFLDAEDLARQLVAWRDEVNTTRPSRATGIIPSVRRAEELARLRPLKIDP
                     AALALRVPIVVGATAYVTHDTHRYSMPSDAIGLAGTLHLYRDRVRIVAGRFEAAHDRL
                     TVPHAIATLPAHRTQHVAAVSGKRGKRYLERQHLLELGPVAHTYLTELTHRRPRLWVQ
                     DVERLHALLQAHGIAATRAAFAQGLAARVFGAEYIAHYLAADEAPGAQERSA"
     gene            1549..2544
                     /gene="istB"
                     /locus_tag="VFQ61_18820"
     CDS             1549..2544
                     /gene="istB"
                     /locus_tag="VFQ61_18820"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HET9956566.1"
                     /translation="MTRATPTPLVDLDGLLKRLHLANTRRVWRDVLARAEQEQWTYEY
                     LLTLLVTEEIAQRQQTRLHRLVHRARFPFLKTIEDFNFTYQSTLRLALLGSALSPDFV
                     TEGRALVLLGKPGRGKTHLAIAIAYRAIQNGFDARFVTAAELIDELSAAFRAGRLGEA
                     LTTYTHPDVLVVDEVGYLTYGTDAANMLFHVVNDRHRRKRAMLFTTNKPVTAWGGVLH
                     DEDLAHAIVDRVLERGRLLTLDGPSLRTKHLGLDDPTASGPSPQVARISGIHAPAFPE
                     PTGIEPANSGFAGAGCREHHDSTRNIIGENSRSLWSDRSRVTLPCAVRSNGHSHQ"
     gene            complement(4023..>5013)
                     /locus_tag="VFQ61_18825"
     CDS             complement(4023..>5013)
                     /locus_tag="VFQ61_18825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HET9956567.1"
                     /translation="PLSYMTGGLTYYYNTDLRGDVVGVNDGVNVNQQINYGPWGNVEN
                     LNGWSLDSTRLGWKGHMWEGGITNLYYVHNRWYDPASRGFISEDPIGLAGGVNTYAYG
                     NNDPVGSIDPTGLSEWLPAGCHKAYDQLSYEEGMTVYVIGFWWWECDDGGGGGSTQPP
                     SGPNGPSTPNTSPSLPTKDQNKIRKLCAEALAYSGPGTFFGGGSLSFVYGIGFEGSTG
                     FYAKSNGTYGKYTSLGWAAGFEISAGLSSGVNYGGMEGLSLAGAGSVGIWGGSVSVGS
                     IIIDPDFPNGGHDSFLTPSPSLTLSGSVGISATPVSARVGLEYTHQTQLGSCK"
BASE COUNT          984 a         1614 c         1493 g          922 t
ORIGIN      
        1 aagttgttgt ctctaaagag gcgcgggccg gaattgcggg ttccgaaaaa actgaccacc
       61 tcgtttccgg aaattctggc cacctagaag gacccaaccc gtcggtggca aatctcgtgg
      121 acgctggcca cgaggcccga ggtgatcgac atgctgaagc gacacgcgat tcacgtgctc
      181 cgagaggcgg ggcacacgct cgaccagatc gccacgttga cggcggtcgg cagacggacc
      241 gtccagcgcg tgatcgatga gcccgaggtc accggcgacc cgccggcccc acgcgggatc
      301 ggtcggccgt cgaaggcggc ggcgtatcgc gcgccgatcg aggcttggct caccgcggag
      361 ccggagctgt tgagcgtgga gttgctccgg cgcgcgaagc tcgcggggta tgagggcgcg
      421 aagagcgcgc tctatgacct cgtgcgggcg gtgcgcgtgg tggtgccgcg gccggtcgtc
      481 cgcttcgagg gcttgccggg cgaattcacg cagcacgatt tcggcgaagt cctcgtctcc
      541 ttcctcgatg ggcacgcgga cccgcgtgca cttctttgcg tcgcgcctca agtactcccg
      601 ctgggtcgag gtcacgctcg tgcccaatca gcaggtcgag acgctcgcgc gcacgctggt
      661 cgatcacttc gccgcgctcg gcgggattcc gctgctcgcg gtcttcgacc gccccaagac
      721 cgtcgcgctc tcgtggaagc agaacggaga ggtcatcgag tggaatccga tcttcgccgg
      781 ggtcgtgctc gatctcggcc tcggggtgga gctgtgctgg ccgcacgcgc cccagcaaaa
      841 aggcgcggtc gaaaacctgg tcggctgggt gaagggcagt ttcttcaagc agcggcgctt
      901 cctcgatgcc gaagacttgg cgcgccagct cgtggcgtgg cgcgatgagg tgaacaccac
      961 gcgaccgtcg cgcgcgaccg gcatcattcc gagcgtccga cgcgcggagg aactggcgcg
     1021 cctgcggccg ctcaagatcg atcccgcggc gctcgcgctc cgcgtgccga tcgtcgtcgg
     1081 ggcgacggcg tatgtcacgc acgacaccca tcgctattcg atgccctccg acgcgattgg
     1141 gctcgccggc acgctgcacc tgtatcgcga ccgcgtacgc atcgtcgcgg ggcgcttcga
     1201 ggcggcgcac gaccgcctca ccgtcccgca tgcgatcgcg acgctgccgg cgcatcgcac
     1261 acagcacgtc gcggccgtct cgggcaaacg gggcaagcgc tatctcgagc ggcagcattt
     1321 gctcgagctc gggcccgtgg cacacacgta tctcacggaa ctgacgcatc ggcggccgcg
     1381 cctctgggtg caggacgtcg agcggctgca cgcgctgttg caagcgcacg gcatcgctgc
     1441 cacccgtgcg gcgtttgcgc aaggcctcgc cgcgcgcgtg tttggcgcgg agtacatcgc
     1501 gcactatctc gccgctgacg aggcgcccgg tgcgcaggag cgctcggcat gacgcgcgcg
     1561 accccgacac cgctggtgga cttggacggg ctgctcaagc ggctccatct cgcgaacacg
     1621 cgccgcgtgt ggcgcgacgt cctcgcgcgc gccgaacagg agcaatggac ctatgagtac
     1681 ttgctcacgc tgctcgtgac cgaagaaatc gcccagcggc agcaaacgcg actgcatcgc
     1741 ctcgtgcacc gcgcccggtt tccctttctc aagaccattg aggatttcaa cttcacgtat
     1801 cagtcgacgc tgcggctcgc gctcttgggc tccgcgctgt ctccggattt cgtgacggag
     1861 ggccgcgcgc tcgtcctcct cggcaaaccg ggacgcggca agacccatct cgccatcgcc
     1921 atcgcctatc gcgcgattca gaatggcttc gacgcccgct tcgtcacggc ggcggagctc
     1981 atcgacgagc tgtccgcggc gtttcgggcc gggcgcctcg gggaagccct cacgacatac
     2041 acccatcccg acgtcttggt cgttgatgag gtaggctatc tcacgtatgg cacggacgcc
     2101 gccaacatgc tcttccacgt cgtcaatgat cgccatcgcc ggaaacgcgc gatgctcttc
     2161 acgaccaaca aacccgtgac cgcgtggggt ggggtgctgc atgacgagga cctggcccac
     2221 gccattgtcg accgtgtcct cgagcgcggc cggctgctca cgctcgatgg accctccctc
     2281 cgcacgaagc acttgggact tgacgatccc accgcgtcag gtccatctcc tcaggtggcc
     2341 agaatatccg gaattcacgc gccagctttt ccggaaccca caggaatcga accggcgaat
     2401 agcggttttg caggggcagg ctgccgagag catcacgaca gtacacgaaa catcatcggt
     2461 gagaatagcc gctcgctgtg gagtgacaga tcacgcgtta cgctcccttg cgcagtccgg
     2521 tctaacggcc atagccacca gtaacacgcc attccggcgc gtgttctccc catactctcc
     2581 ccactgtcgc ggaagcctga ttgtcggcag agcttctcat gcacgtaccg agacaccgat
     2641 tcgcgttgat caccaccttg aacggcgtgc gccctgtcag agtacttcac acatgggagg
     2701 gcctgctttt tcgggcgagt ccgaaggttt agcgagcatt cgagcccaga cggcaacgag
     2761 cggtgaccgc aacgaaaggc aacgacgagt tgcccaccga gcaacccgag cggtagtgct
     2821 ctattgttca cccaaggacg ggttacttga tccttgctca actataccgt gagtcgcacg
     2881 tgcaggcgcg ccagagcaga tgagccagcg tgcgctgata gcgaccgtgc acgcgagtgc
     2941 tcgcatggcg acaaacgggg cacgcaacca acacgtcgcc gtacccacgc cgccacgatg
     3001 agctcatccg acggtaaggc gatctgatcc agcttcactt cgataggtga cgggcacacc
     3061 gccgtttccg gtgagagtat catcggtact gctgccgcaa gacgactctt ggacgctagc
     3121 aagtcgccag ctccatcacc aaagctgggc agaacagatt ccatcccgcc aacaaagggg
     3181 cgtaacaggt tcagccacag tcgttcgagt cgccctcgga gctcaacaca atctcacggc
     3241 aaagctcgcg tctatgagtg cgtgatcaga tgtgtcgcag tagacctccc gaggaaccac
     3301 cacaccaaga ggaggatgac gacggccaag atgttccacg ccaatgctat ccgagagtta
     3361 ctgagcaacg ctggaatcgg ccggtccaaa aaggcaagcc gtaacatggg ttcaacaaag
     3421 cttcggcgaa agccatcatg ccaaatgacc gtggtgagga acacggcgac ggcaagaacc
     3481 agcaccagta ggcgcaatgg attcagaaag ggcatactct atctcccaga gcgagacgca
     3541 gacgaatact tcacttcgtt atagcgtttg ggacgtcgcg cctgcaaatc acgtatagga
     3601 cggcgacgcc gttaccgacc gtgtaaacta cctaacgagc gacactgaaa taatggccgc
     3661 gaccactgct atcacactgg cccacacgta cttgcgtcca ctcttcttga catttccaaa
     3721 atagaacggc atggtcgggt ctttgcggtg ctgtgacgcc ttccataagc acacaaggtg
     3781 ggctatcacg ccgagaacga agaccactat ggcaagaccg cgcattggat tatgggattg
     3841 agtatttgac gaaacgctgc tcgctacagg tgcgcagggc tcgtagcgta agtgattgcg
     3901 ttctcgcgcg agccctgtcc tgcgacgagg tagagtctta gaggaactag cagccgcccc
     3961 acctcaggtg gcggaagcgg cggctgttgt tcatcgcggc tacttagttg gctgatctag
     4021 aactacttgc aggagcccaa ctgcgtttgg tgcgtgtact ccaaaccaac tcgtgcggaa
     4081 acaggtgtag cggaaatgcc cacggaacca gaaagtgtta gactgggact tggcgtcaag
     4141 aagctatcgt gaccgccatt cgggaagtcc ggatcgatga tgatgctgcc cacggacacg
     4201 gagccacccc atataccgac gctccctgcg cccgctaaag acagcccttc catgcctccg
     4261 tagttgacac cgctgctaag accggcgcta atctcgaagc cggctgccca acccaggctc
     4321 gtgtacttcc cgtacgttcc gttagacttg gcgtaaaatc ccgttgaacc ctcaaagcca
     4381 atcccgtaca caaagctgag cgaaccaccg ccgaagaagg tgccaggtcc ggagtaagcc
     4441 aaggcttcag cacaaagctt tctgattttg ttttgatcct tggttggaag gctgggtgat
     4501 gtgtttggcg tactgggacc gttcgggccg ctcggcggct gggtcgagcc gccaccacca
     4561 ccatcatcgc actcccacca ccaaaagcca atcacgtaca ctgtcatccc ctcctcatag
     4621 gacagctggt catatgcctt gtggcatcct gctggcaacc actcactcaa gccagtcgga
     4681 tctattgacc ctaccggatc gttgttcccg tacgcgtacg tattcacccc gccagcgagg
     4741 ccgatcgggt cttctgatat gaatccgcgg ctcgcaggat cgtaccagcg attgtggacg
     4801 tagtagaggt tcgtgatccc gccttcccac atgtggcctt tccaccctag ccgtgtgctg
     4861 tcgaggctcc aaccgttcag gttctccacg ttgccccacg ggccatagtt gatctgctgg
     4921 ttcacgttga cgccgtcgtt cacgcccacg acgtcgcccc gcagatccgt gttgtagtag
     4981 tacgtcaggc ctccggtcat gtagctcagc ggc
//
LOCUS       DASTKU010000540         3901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_551081, whole genome shotgun sequence.
ACCESSION   DASTKU010000540 DASTKU010000000
VERSION     DASTKU010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551081"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="VFQ61_18830"
     CDS             <1..1402
                     /locus_tag="VFQ61_18830"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET9956568.1"
                     /translation="IAPNKPFTAWAAWLVAMSSGNLDDADAAIQRARKLGAPPEVLAK
                     MTAATADARPWSDVVLYWTVRILVGWFGLALLLVIVGSALSRATLSTAENWTAESAKR
                     GAALRSVYRGVLVVCSALYYASLPLVLLAVVLLAGGLIYGMFAIGYIPIKLGAIAAFM
                     VFATGLAIIKSLFFRPSSEAPGPEVDLAKEPGLRAVLDEVAAQIGTRPVDVVYMTADT
                     NLAVFERKKRRCLVLGAGVLDGMPLDSFKAILAHEYGHFSNRDTAGGGFALSVRRSLI
                     TFIIGLAQSGQASPWNPAWWFARGFYNVFLRISQGASRLQEIMADRLAANAYGGLAFA
                     NGLKHVVACDLHFEAHVNKEIQRALKEKEPLKGLWSPLAGDHGDARELEAALSREPSP
                     YDSHPSPRDRIRWVEGIPGAAPCSSNPDAKAWDLFQNRAHREREATLYIYGRLAQNGV
                     KLPALPATTPNGDSAR"
     gene            1540..2247
                     /locus_tag="VFQ61_18835"
     CDS             1540..2247
                     /locus_tag="VFQ61_18835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET9956569.1"
                     /translation="MPRSSSTDPALKKLGLLAPQPVRQSAAPSGREASEAPKNKRKRN
                     KPPTIQVRRIHRRDLNAVWDFLKLSFRDVNRATVEYQRPKTKQRFFETYEEEGVEQLL
                     FLVERDIVAYAECTFEIMGKDTWLNPSYFERRDMRPLYVEELAVHPDWHGRGVGSFVL
                     EQLQHLARVRGLTHLVLEVAENNENALGWYRKRGFRKLDAAIFLAQTVNTEPELLPPR
                     ELAPRHLPSRSEGGSDE"
     gene            complement(2274..2723)
                     /locus_tag="VFQ61_18840"
     CDS             complement(2274..2723)
                     /locus_tag="VFQ61_18840"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9956570.1"
                     /translation="MPDLRSRIAREADVPLLLAMMESFNLLEGIDWDVHAKEPALRTL
                     LRERELGLVALLEGPDGPFGYYVLTWGYDLEWNGRDAFLTELFLVPSARGKGNGAHAL
                     ELLESLARKHGARALHLMVRHENIVARRLYMGHGYRSPERLFLSKEL"
     gene            complement(2853..3764)
                     /locus_tag="VFQ61_18845"
     CDS             complement(2853..3764)
                     /locus_tag="VFQ61_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956571.1"
                     /translation="MRLRWAALVLLAFGCRAVAPAEAPQENPSPPSSAKPPGSTPKSA
                     PIASTTSTAPAPERPPSYYGGMEPPRRTPAIAREEAHLEENYKEVPLFESLPPSISID
                     GIAAALPPDWHESREWLREWRPGDKPSELVRAAYLDSQEHYYLVWFHSNLNQLALVFD
                     RRGRVTHRQAFSRARPTFRDVLALETRAERDRGATAHELVVFRITTMSLCCLPTDLEV
                     YALGPMGKLRLILDIPKDHVDVGPGVRYAFLNHVDFVDQTAVVTQLEPESPLRWEFSY
                     SPARGRFVPDAATVQSMKEERTRWQSH"
BASE COUNT          711 a         1202 c         1223 g          765 t
ORIGIN      
        1 gatcgcgccg aacaaaccgt tcaccgcgtg ggcggcttgg ctcgtggcca tgtcctcggg
       61 gaacctcgac gatgcagacg ctgccattca acgagcgcgc aagctaggcg cgccgcccga
      121 ggtgctcgcg aagatgaccg ccgctactgc cgacgcgcgc ccctggtccg atgtcgtcct
      181 ctactggacg gttcgtatcc tggttggctg gttcggcctg gcgctactct tggtaatcgt
      241 gggcagcgcg ctcagccggg cgacactttc gaccgcggag aactggacgg ccgaatcggc
      301 caagcgcggt gcggcgctgc ggagtgtata tcgcggcgtg ctggtggtgt gctctgcgct
      361 gtactatgcg tcactgccgt tggtgctgct ggccgtcgtc ttgctggcgg gcggcttgat
      421 ttacggcatg ttcgcgattg gctacatccc gatcaagctc ggcgcgatcg cagcgttcat
      481 ggtgtttgcc accggtttgg cgatcatcaa gagcttgttt ttccgaccaa gcagcgaggc
      541 gccgggtccc gaggtagacc tcgcgaaaga gccagggcta cgtgcggttc tggacgaggt
      601 tgcggcgcag attggcactc ggcccgtcga cgtcgtctat atgactgccg acacgaactt
      661 agccgtcttc gaacgtaaga aacggcgttg ccttgtgctc ggcgcggggg tgctcgatgg
      721 gatgcccctc gattccttca aggccattct ggctcacgag tatgggcact tctcgaatcg
      781 cgatactgcc ggcggaggct tcgcgctgtc ggtccgccga tcgctcatca ccttcatcat
      841 cggtctcgcg caatcgggac aagcctcgcc ttggaacccg gcctggtggt tcgcgcgggg
      901 attctataac gtgtttctgc gcatctctca gggcgcatca cgtttgcaag aaatcatggc
      961 ggaccgcctc gcggcgaatg cttatggcgg tttggcgttt gccaatggtc tgaagcatgt
     1021 cgttgcgtgc gacctccatt tcgaggccca cgtgaacaag gaaatccaac gcgcactgaa
     1081 agagaaggag ccactgaagg gtttgtggtc accgctcgcg ggcgatcatg gtgacgcacg
     1141 agagttggag gcggcgctca gtcgcgagcc gtctccctat gacagccacc cgtcaccgcg
     1201 ggatcgcatt cgttgggtag aaggcatccc cggagctgct ccttgctcga gcaatccaga
     1261 tgccaaagca tgggacctgt tccaaaaccg cgcccatcgg gagcgtgagg ccacgctcta
     1321 tatatacggg cgcctggccc agaacggcgt gaagctaccc gcgctaccgg ccaccacgcc
     1381 caacggcgat tcggcgcggt agcccggcgg ctggccgcag gcggtacaca tccagccccg
     1441 cgtgtcggct ttcgctgacg gaacggcttg ttcgcccggg atttgctgga gctccagctc
     1501 gatgggggcc ggcgtcgctt ttgggctatc atcccattcg tgccccgttc ttcatcgacc
     1561 gatcctgccc tgaaaaagct ggggctgctc gctccgcaac ccgttcgaca gtccgcggct
     1621 ccctctggcc gggaggcgag cgaggctcct aaaaacaagc gtaagcgcaa caagcctccg
     1681 acgattcagg tccgacgcat ccatcggcgc gacttgaacg ctgtgtggga cttcctaaag
     1741 ctttcttttc gggacgtgaa ccgcgccacc gtcgagtacc agcgccccaa aaccaagcaa
     1801 cgcttcttcg aaacctacga agaggagggc gtcgaacagc tgctcttttt ggtcgaaaga
     1861 gacatcgtgg cgtacgcgga gtgcacattc gagatcatgg gcaaggatac ctggctcaat
     1921 ccaagctact tcgagcggcg ggacatgcgc ccgctctacg tcgaggagct tgccgtgcat
     1981 cctgattggc atgggcgggg cgtggggtcc ttcgtgctcg agcagctgca acacctcgct
     2041 cgagtgcggg gcttgactca cctggtgctc gaggtcgccg aaaacaacga gaacgcgctc
     2101 ggatggtacc gaaagcgggg attccgcaag ctcgatgccg cgatctttct ggcgcagacc
     2161 gtcaacaccg agcccgagct gctcccacct cgcgagctgg cgccgcgcca tctgccgtcg
     2221 cgttccgagg gcggatccga cgaatagtgg agttcgccga cgtgccgagt gagtcatagc
     2281 tccttgctga ggaacaggcg ttccgggctc ctatagccgt ggcccatgta aagacgcctc
     2341 gcgacgatgt tctcgtgacg caccatcaag tgcaatgccc gcgcgccgtg cttgcgcgcg
     2401 agtgattcga gtaattcgag cgcgtgcgct ccgtttccct tgccgcgtgc ggaggggacg
     2461 aggaagagtt ccgtgagaaa cgcatcgcgg ccgttccatt cgagatcata cccccaagtg
     2521 aggacgtagt agccgaatgg tccgtctggc ccttcgagga gcgccacgag gccgagttcc
     2581 cgctctcgca gcagggtacg caacgcgggc tccttcgcgt gcacgtccca atcgatcccc
     2641 tcgagcaggt tgaaggattc catcatcgcg agcagcaagg gcacgtcagc ttcacgagca
     2701 atcctagaac gtaaatcggg catacccctg atacgctcga ggaactcgcc ggttacgaat
     2761 ctttttccgt gtggcggtct ccccagtcag ggcatatgcg cgggtgacct cgcgtgtcac
     2821 tgcactaact tcctagaaca caagcgccgg cactagtgcg actgccatcg cgtccgctct
     2881 tccttcatgc tttgcacggt cgccgcatct ggcacgaagc ggccgcgcgc agggctgtag
     2941 gagaactccc agcgtaaagg gctctcgggc tcgagttgcg ttacgaccgc ggtctggtcc
     3001 acgaagtcga cgtgattcaa gaaggcgtaa cgtacgccag ggccgacgtc cacgtgatct
     3061 ttgggtatat cgagaatcaa acgcagtttg cccatgggcc cgagcgcata cacttccaga
     3121 tcggttggta ggcagcacaa actcatggtc gtgatccgaa acaccacgag ctcgtgcgcg
     3181 gttgcgccgc gatctcgttc tgctcgggtc tccagcgcca atacatcgcg aaaggtgggg
     3241 cgcgcgcggg agaaggcctg tcgatgagtc acgcgcccgc gccgatcgaa gaccaaggcc
     3301 aactgattta gattcgagtg aaaccaaact aggtagtaat gctcctgaga atcgagatat
     3361 gcggcgcgta ccaactccga gggcttgtct ccggggcgcc actcccgcag ccattcccgc
     3421 gactcatgcc aatctggagg gagtgccgcg gcgatcccgt cgatcgatat agaaggagga
     3481 agcgattcga acaacggcac ctccttatag ttctcctcca ggtgggcttc ctcgcgggct
     3541 atcgcgggcg tccgacgcgg tggctccatg ccgccgtagt aggacggtgg ccgttccggc
     3601 gccggtgctg tgctggtggt ggaagcgatg ggggcgctct tcggcgtcga gccgggcggt
     3661 ttcgccgatg aaggcggact tgggttctcc tgtggcgcct ccgccggagc gacggcacgg
     3721 catccgaatg ccaacaacac caacgcagcc caccgtagac gcatggggca accatagccc
     3781 gaaaaccccg ttgtggttag agcacggctt tttggacgat attgcgctcg gtggcggtca
     3841 gcttggtccg ctattgtttg ggcggcaccg acgccaatcg acgcgctccc tcccaatgct
     3901 g
//
LOCUS       DASTKU010000541         7046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_996608, whole genome shotgun sequence.
ACCESSION   DASTKU010000541 DASTKU010000000
VERSION     DASTKU010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996608"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..768)
                     /locus_tag="VFQ61_18850"
     CDS             complement(34..768)
                     /locus_tag="VFQ61_18850"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9956572.1"
                     /translation="MDLHGRTVLLTGGSSGIGLALAQRFVRAGSRVIVTGRRDASLAA
                     ARAACPGLETLVNDVGSEVDRARLASHLRDQKIPIDVLINNAGIQNRVDPTRLEAWAQ
                     LSEEIRINLEGPIHLTSLLLPLLHERAQAYVVNVSSGLAFVPLAGVPIYSATKAGIHS
                     FTQSLRHALAPTNVRVIEIIPPAVRSNLGGSHDFGVPTEEYADSVMRGLAEDREEIAY
                     QFSQRASQASRAELDAMFVEMNARRS"
     gene            828..1571
                     /locus_tag="VFQ61_18855"
     CDS             828..1571
                     /locus_tag="VFQ61_18855"
                     /inference="COORDINATES: protein motif:HMM:NF033082.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HET9956573.1"
                     /translation="MQLERQLRDLGLECGSMVMVHASMRAVGGRAEALVSALLNGLGP
                     EGTLMAYLDFELTEQTPYFDLQRSPAAADHGVMAEVIRCWPGAVRSASPGASVAAVGA
                     RAEWLCADHSLQYGYGPGSPFAKLVEARGQVLLLGSHLDHVTLLHYAEHLAQLPNKRV
                     VRYQVPTPNGALDIEEFDTSEGVVEAMPHDYFDRTIRDFIATGAAQCGRVGGAPSYLL
                     PAAELTAFAVRKMERELAPASLSFKGARP"
     gene            complement(1552..2409)
                     /locus_tag="VFQ61_18860"
     CDS             complement(1552..2409)
                     /locus_tag="VFQ61_18860"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9956574.1"
                     /translation="MSESPTAKDWIGSRGEKWSAQAESMEAMLTPIDRPLIEALRLEQ
                     PTRVAEVGCGAGGTAMELRRRAPAGTSIHGFDISPKLIELARRRITAGSLQGSTENTL
                     AFELADVSTHIPNQRYDRLVSRFGIMFFDDPAGAFSNLLRWLEPGGRIAFAVWGPLAE
                     NDWFRSVRDVVALFAEMPEPPPDAPGAFRYADVSSLLSSLQHAGFSRLEAQAWRGTLS
                     IGGGLQPAQAATFVLSAFSSFADRLAAVGGGAFDRAHSALTTHFTERQRAGATGESAF
                     VHIVTGERP"
     gene            2635..3546
                     /locus_tag="VFQ61_18865"
     CDS             2635..3546
                     /locus_tag="VFQ61_18865"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9956575.1"
                     /translation="MIYDAIIVGGGPAGLSGALALGRAVKRVRLCDSGPRRNAAAEQI
                     HNFVTRDGTPPEQFREAARQQLGSYEKVELSSDRVEEISGSRGAFRVSLSSGQVQARR
                     VLLCTGMVDEMLDVEGFRELWGQAIFQCPYCHGWEVKNRSWGYWARTPQMLHFPLQLR
                     GWSKDVVVFTGGAFELPEEVASRFQAVGVACDQRPIRRLISRDGKLEAIEFTDGSQRA
                     CEVLFSHPPQRQVDLVRQLGLALDEDGFVRVDPVTRETSIPGIYAAGDLQSKMQGAII
                     AAASAAHAAAMLNHELTVELALSGELG"
     gene            complement(3583..3999)
                     /locus_tag="VFQ61_18870"
     CDS             complement(3583..3999)
                     /locus_tag="VFQ61_18870"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9956576.1"
                     /translation="MASIYEEIVTLATPSQAWQAVRDIGALHTRLVPGFVTHTQLEGS
                     TRIVTFGNGMVVREPIITVDDTHRRLVWGAESERLTHYNASVQVFESGTGSRIVWIAD
                     LLPDNAASFIQSMMQSGMAAMKRALDETNHDSNSKD"
     gene            complement(4010..4855)
                     /locus_tag="VFQ61_18875"
     CDS             complement(4010..4855)
                     /locus_tag="VFQ61_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9956577.1"
                     /translation="MVLVTVLHQSPSIAVLDYRCDAKPDDPSYDEHHTRYSVSYVRRG
                     SFGYRTRGRAYELVPGATLLGRAGDDFRCSHDHHIAGDECLSFQFSEEFAETFGGSAK
                     SWWSGAVPPVSALMALGELASHTASPRCALGLDELAMYFSARLFACVSGQLEGPVRVH
                     ERDRKRAVDAALLIEARSTEALNVEHVAATIGLSPFHFLRTFRAVLGVTPHQYLIRCR
                     LRRAASLLTTDLGITEIAYEVGFGDLSNFVRTFHRACGASPRAFRRAARGDRKIFQER
                     LRRLS"
     gene            complement(4932..5861)
                     /locus_tag="VFQ61_18880"
     CDS             complement(4932..5861)
                     /locus_tag="VFQ61_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9956578.1"
                     /translation="MPQRRNDSATQRSKIGTPDGSSHLEDLAVFVLVARATSFARAAQ
                     RAGLSTSSVSRAVARLEAKLGVRLLQRTSRTVACTEEGRQLCLQAGPLVDELQQALVL
                     AADRRSQPVGVLRVTAPAFTGATRVASALAAFALAYPEISVELDATNTTRNLIEDGYD
                     LAIRVGPVTDGDLVARKLWDVESALFVSRELIAPRPPYRVERAALERAPAVVTRASSV
                     WRFLAPDGSRVEITPNARFVVNDPRAAVEAARQGLGYVLAPADAVSERDRALVRVRPS
                     FGEPESREVFAAYPSRRLLPVRVRLALDWLAKH"
     gene            5884..6600
                     /locus_tag="VFQ61_18885"
     CDS             5884..6600
                     /locus_tag="VFQ61_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9956579.1"
                     /translation="MISNASLVMVVGGSSGIGLGVARAVVQRGGSVLIVGRSREKLEQ
                     AQGQLGSRARAIRADVTVESEVARLAAQSGAVDHLVVTAVAGAYAPIAQLELEAARSV
                     IDSKLVAAMLLAKHVGTKLPAGGSITLTSGIAKDRPMPGGAMVAAVNGALSAFARALA
                     LELAPARVNVVSPGWVDTPVWDRLAGSAKQEKWDSLARRLPVGRIGSVEDLAPAYLFL
                     MENGFTTGTTIHVDGGHALS"
     gene            6658..7023
                     /locus_tag="VFQ61_18890"
     CDS             6658..7023
                     /locus_tag="VFQ61_18890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HET9956580.1"
                     /translation="MTPNRIHRGDLFWVEAEDAGGLAGGAPHPHLVVQDDLFNHSRIQ
                     TVVVCALTTNLQRASEPGNVLLDPGEGSLPRQSVVVVSQIASIQKAHLGARIGALSRS
                     RVEQVLQGLLFQQRSFVTR"
BASE COUNT         1304 a         2133 c         2277 g         1332 t
ORIGIN      
        1 cgcgcactct acgcgattga tgcttggggt tgatcaggat ctgcgcgcgt tcatctcgac
       61 gaacatggcg tcgagttcgg ctcgagaggc ttgactcgct cgctgagaga attgatacgc
      121 gatttcttcg cgatcttctg caagcccccg catcaccgag tcggcatatt cttcggtggg
      181 caccccgaag tcgtgagagc cgccgaggtt cgaacgtact gccggcggaa taatctcgat
      241 cactcgcacg ttcgtgggtg ccaaggcatg gcggagcgac tgcgtgaacg agtggattcc
      301 ggccttggtc gccgaataga ttggtactcc cgccaaaggt acgaaagcga ggccggagct
      361 gacatttacg acgtacgcct gcgcacgctc atggagcagc ggcagaagca ggctcgtgag
      421 gtgtattggt ccctccaagt tgatccggat ctcctcgctc agctgcgccc atgcttccag
      481 tcgagtggga tcgacccgat tctggattcc ggcattgtta atcaatacat cgatggggat
      541 cttctggtca cgcaaatgag aagcgagccg ggcgcggtct acctcactac ccacatcgtt
      601 cacgagcgtt tcgaggcccg ggcatgcggc ccgggcggct gccaacgaag cgtctcgccg
      661 acctgtgacg ataaccctgc ttcccgcgcg aacgaagcgt tgggccagcg ccaaaccgat
      721 gccactcgag ccacccgtca gcaagactgt acgaccatgc agatccatgg ggccagcttc
      781 catcgacttg ccaaacggcg caacaacgat cagaaacggc gacgcacatg caactagaac
      841 ggcaactgag ggacctaggg ttggaatgcg gcagcatggt catggttcac gcctcgatgc
      901 gggccgtggg cggacgggcg gaggcgctgg tgagcgcact tttgaacggg ttggggcctg
      961 agggaactct aatggcctat ttggacttcg agctgaccga gcaaactccc tatttcgact
     1021 tacagcgctc gccggcagcg gcggatcacg gggtaatggc ggaagtaatc cgttgttggc
     1081 cgggagccgt tcgctctgcc agtccgggag cttcggtcgc ggccgtcggt gcccgggcgg
     1141 aatggctctg cgcggaccat tcacttcagt atggttatgg tcctggatca ccgtttgcca
     1201 agctggtcga ggcgaggggc caagtcttgc tcttgggctc tcacctcgac cacgttaccc
     1261 tgcttcacta cgccgaacac ttggcgcagc ttcccaacaa gcgggtggtc cgttaccagg
     1321 tccccacgcc caacggcgcg ctcgacatcg aggaattcga caccagcgag ggtgtcgtcg
     1381 aggccatgcc acacgactac ttcgatcgaa ctattcgtga tttcattgcc acgggggcgg
     1441 cccagtgtgg tcgggtcggt ggagcaccca gctatctttt gcccgccgcc gagctgaccg
     1501 cctttgcggt gcgcaaaatg gaacgcgaac tcgcgcccgc gtctctctcc ttcaagggcg
     1561 ctcgcccgtg acgatgtgca cgaacgccga ctcacccgtg gccccggcgc gctggcgttc
     1621 cgtgaagtgt gtcgtgagcg cgctgtgggc gcgatcgaat gccccccctc cgacggccgc
     1681 caggcggtcg gcaaaggaag agaatgccga taggacgaac gtcgcggcct gggccggttg
     1741 taggccaccg ccgatggaga gcgtgccgcg ccaagcctga gcctcgagcc gcgaaaagcc
     1801 cgcgtgctgt agcgagctga gcaacgaact cacgtccgca tagcgaaacg ctccgggtgc
     1861 gtccggcggt ggctcgggca tttcggcgaa aagcgcgacc acgtcacgca cgcttcggaa
     1921 ccagtcgttc tccgcgaggg gaccccagac ggcaaacgca atccgccctc cgggctctag
     1981 ccaacggagc aagttcgaga acgcacccgc tggatcatcg aagaacatga tcccaaaccg
     2041 cgaaaccagc cgatcgtagc gttggttggg gatgtgggtc gagacatccg ccagctcgaa
     2101 cgcgagcgtg ttctcggtcg agccctgcag ggaccccgcg gtaatgcgtc gtctggcgag
     2161 ctcgatgagt ttcggtgaaa tatcgaatcc gtggatgctc gtgccagcgg gagctctacg
     2221 ccttagttcc atggcggttc cgcccgcgcc acacccaacc tccgcaaccc tcgtgggctg
     2281 ctcgagtcgc aaagcttcga tcaagggacg atcgatgggg gtcagcatgg cctccatgct
     2341 ctctgcttga gccgaccact tctctcctct cgacccaatc caatctttcg ccgtggggct
     2401 ttcactcatg gcaaactccg aagaaagcgc cgcgaactcc acggatgccg cagagttact
     2461 agcgctgctg aaagatgcca ttcgttcgat gcaggagcca atcacttctt cggtggaggg
     2521 ccctcaagaa accggaggtg tagcaactgg cacagcacct gcccgatctt ccgatcgtca
     2581 tagagcttct cttctggcat tagaacgggc agtgtagcgc cctggagtga gccattgatc
     2641 tacgacgcaa tcattgtggg aggcggcccc gcgggcctgt caggtgcgct cgccctcggg
     2701 cgagcggtga aacgagtgcg gttgtgtgat tcggggccgc gccggaatgc ggccgccgag
     2761 cagattcaca acttcgtcac gcgcgatgga actccgcccg agcagtttcg agaagcggcg
     2821 cgacaacaac ttggctcata cgagaaagtc gagctcagca gcgatcgcgt ggaggagatt
     2881 tccgggagtc gcggggcctt tcgagtgagc ctctcttcgg ggcaggtgca agcacgtcgg
     2941 gtgctgctgt gtactggcat ggtggacgag atgctggatg tagaggggtt ccgggagctg
     3001 tgggggcagg ccatcttcca atgcccttat tgccacggtt gggaggtaaa gaatcgaagc
     3061 tggggatatt gggcgcgtac gcctcaaatg cttcacttcc ccttgcaact gaggggctgg
     3121 tcgaaagatg tggtggtgtt cacgggcggc gcgttcgagc tccccgaaga ggtggcttca
     3181 cgcttccaag cggtcggtgt ggcatgcgat cagcgtccaa tccgacgcct gatctcccgt
     3241 gacgggaagc tcgaggcgat cgagtttacg gacggaagcc agcgcgcgtg tgaggtcttg
     3301 ttcagccacc cgccgcagcg tcaggtcgat ttggtgcgac agctcgggct cgctctggac
     3361 gaagatggct tcgtgcgagt ggatcccgtc acgcgtgaga cgtccatacc gggcatttac
     3421 gctgctggag atctccaatc gaagatgcag ggggcgatca tcgcggccgc ttctgccgct
     3481 catgctgcgg cgatgctgaa tcatgagctc acggtcgaac tcgcgctgag cggcgagttg
     3541 gggtgagggt aacccacggc gttcgggtcg aaccgttcga attcaatctt tcgagttcga
     3601 atcatggttc gtctcgtcca gggcccgctt catcgccgcc atgccactct gcatcatcga
     3661 ttgaatgaat gaggccgcgt tatcgggcag cagatcggca atccagacga tccgactgcc
     3721 cgttcctgat tcgaacacct gtaccgatgc attgtagtgg gtgaggcgct cggactcggc
     3781 tccccaaacc agcctgcgat gcgtgtcatc taccgtgatg atcggctcgc gcactaccat
     3841 gccgttccca aacgttacta tacgagtgct cccttcgagc tgagtgtgcg tcacgaatcc
     3901 cggtactagg cgcgtgtgca aagctccgat gtcccgaacg gcctgccaag cctgggaagg
     3961 ggtcgcaagc gtcactatct cttcgtagat cgaggccatg acgcgattct tacgagagcc
     4021 gcctcagacg ttcttggaaa atcttgcgat cgcctcgagc cgcgcgccgg aaggcgcggg
     4081 gggacgcgcc acacgcgcgg tgaaatgtcc taacgaagtt ggataggtct ccgaagccaa
     4141 cttcgtacgc aatttcggtg atgcccaagt ctgtggtcag gaggcttgcg gcgcgtcgca
     4201 gtcggcagcg gatcagatat tggtgaggtg tgacgccaag cacggcccgg aacgtgcgca
     4261 ggaaatgaaa tgggctgagc ccgatggtcg cagcgacgtg ttccacgttc aatgcctcgg
     4321 tcgagcgtgc ctcgatgagc agcgctgcgt ccacggcgcg tttacggtcc cgctcgtgga
     4381 ctcgaaccgg cccctcgagc tggccggaaa cgcaggcgaa cagccgagcc gaaaagtaca
     4441 tcgcgagttc atccagaccc agtgcgcacc tcggagatgc agtatgcgaa gcgagttcgc
     4501 cgagcgccat cagtgcggat actgggggta ccgcgccact ccaccagctc tttgccgagc
     4561 cgccgaacgt ttccgcgaac tcttctgaaa attggaagga gagacattcg tctcccgcaa
     4621 tgtggtgatc gtgagaacaa cggaagtcat cgccggcccg accgagcagg gtggcaccgg
     4681 gaacgagttc gtacgcgcga ccacgggttc ggtagccgaa gcttccccgg cgcacgtagg
     4741 acacggagta tcgggtgtgg tgttcgtcgt aagacggatc gtccggcttc gcgtcacacc
     4801 ggtagtccaa cacggcaatc gacggagatt gatgaaggac ggtgactagc accacgctga
     4861 ttaccagtca ttggtagctc gagcaactcg gtttctgccg cacgggacgt ggcgcttggt
     4921 gttgtgtcgg ttcagtgctt ggcgagccag tcgagcgcga gtcgcacccg cacgggtaag
     4981 agtcgtcggc tcggataggc cgcgaacacc tccctggatt caggctcccc gaagctaggt
     5041 cgcacccgca cgagagcgcg atcgcgttcg gataccgcgt ccgccggggc gagtacgtag
     5101 ccaagccctt gtcgtgcagc ttcgacggcg gcacggggat cgttcacgac gaagcgggcg
     5161 ttgggtgtaa tctcgacacg cgagccatcc ggcgcgagaa agcgccacac cgagctcgcc
     5221 cgcgtcacca ccgctggcgc ccgctccagc gccgcgcgtt ctacgcgata gggcggtctc
     5281 ggggcgatca actcgcgaga cacgaacagg gctgattcga cgtcccatag tttgcgcgcg
     5341 acgagatccc cgtccgtgac gggtccgacc cgaatcgcca aatcgtatcc gtcctcgatc
     5401 aggttgcgtg tggtgttggt ggcgtcgagc tcgacgctga tttcaggata agctagggca
     5461 aacgccgcga gcgcggaagc gacgcgcgtg gcgccggtga acgcgggggc cgtgacgcgc
     5521 agtaccccca ccggttggct tcgtcgatcc gccgccagca ctagcgcctg ctgaagctcg
     5581 tcgaccaagg gccccgcttg caggcatagc tgccgccctt cctcggtgca tgcgacggtg
     5641 cgtgaagttc gctgtagtag gcgaactccg agctttgcct ccaagcgcgc aacggcgcgg
     5701 ctgaccgaac tcgtagagag cccggcacgt tgggcggcgc gggcaaagct cgttgcccgc
     5761 gccaccagca cgaacacggc aaggtcctcc agatgagaag acccgtccgg cgttccaatt
     5821 ttggaacgct gcgtggcaga atcgttccta cgctgcggca tcgcaacgat gtactaccag
     5881 cccatgattt cgaatgcatc tttggtcatg gtcgtaggcg gttcctccgg cattggtttg
     5941 ggggtggctc gggctgtcgt gcagcggggc ggctcggtac tcatcgtcgg tcgctcacgg
     6001 gagaagctgg agcaagcgca gggccagctc gggtcacggg ctcgcgccat aagggccgac
     6061 gtgacggtgg agtccgaggt ggcgcgcttg gcggctcagt ccggggccgt cgatcacctg
     6121 gtggtcaccg ccgtcgccgg tgcttatgca cccattgccc agctggagct cgaagcggcg
     6181 cgcagcgtga tcgactccaa gttggtggcg gcgatgctac tggccaaaca cgttggcacc
     6241 aagctacctg ccggcggatc tatcacgttg acctctggca tcgcgaagga ccgcccgatg
     6301 cccgggggag ccatggtcgc tgcggtgaac ggcgcattga gcgcgttcgc gcgcgcgctg
     6361 gccctggagc tcgcgccggc tcgggtcaac gtagtgtctc ccggttgggt cgatacaccg
     6421 gtttgggatc gccttgctgg cagtgccaag caggaaaagt gggactcgct agcccggcgt
     6481 ctgcccgttg gtcgcatcgg cagcgtggaa gacttggctc cggcctacct gttcctgatg
     6541 gagaacgggt tcacgaccgg aaccacgatt catgtagacg gcgggcacgc gctttcttag
     6601 gcggcgacca cgcaaccccc cgttgccggc ggccggcttt cgtgtacacg tctctcggtg
     6661 actcccaatc gcatccaccg tggggatctg ttctgggtcg aagcagaaga cgcgggtggg
     6721 ctggcggggg gcgctccgca cccgcacctc gtggtgcaag acgatctctt caatcactcg
     6781 cgtatccaga ccgtcgtggt ctgcgcgttg accaccaacc tgcagcgcgc gagtgagccg
     6841 ggtaacgtgc tactcgaccc aggagagggc agcctgccgc gtcagagcgt ggtggtcgtc
     6901 tcacagatcg cctcaatcca gaaggcacat ctcggagccc gcatcggggc gttatctcga
     6961 tcacgggtcg agcaggtctt gcaggggctg ctgtttcaac agcgctcgtt cgtgacgcgc
     7021 tgaaggtagg cgggctgcga ttgtag
//
LOCUS       DASTKU010000542         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_105536, whole genome shotgun sequence.
ACCESSION   DASTKU010000542 DASTKU010000000
VERSION     DASTKU010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105536"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2030
                     /locus_tag="VFQ61_18895"
     CDS             <1..2030
                     /locus_tag="VFQ61_18895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956581.1"
                     /translation="LCIALGGLAACIMAECFAFHRGKQLLSLTAQDLSTQLTELEEQL
                     FSHRDWIQSVTLLFPSWANSGAGPNTQALERVLTLLRKAELGYPVGLLTARTPALLST
                     LIVKSCVLPLELGSIAVVGAQSDAPPIPASREDPAREFFTQADLLHRNADPGLDFRVE
                     HLDETRSPAAVVRALHHEPIGTLIFRGHSRPYCGLEGRLCASPASQQPTQAECVLGLD
                     CYDASWPKVDPRSFGAELVVLETCSALTASTTAWEDGGAPIAILASESRATAVLTTAA
                     PTVMRDVSFLLSALTRTRTVGEAGVLFDQSARSHQGCPSVFLLGDPDQPLPNCFFGAV
                     PEIGREHDAGAVAALGGSDTLLPRPIYQRYALPGVREKTTVRVTGDLNDVEQASLCLI
                     PGASHSELWLRSRRRNLDVCISPTPVPRFPTEVLSAAVALPARLLSFTGLASAGVPGL
                     LQACRSVVQCQAHLESLADRATAYDFEGYVRSTILDWWAAHRDFLESTLTQRGQSFWI
                     DNVCSPTGPTSRHSTCFPCAQCGRASVTIRSYDVFPAGARQARECEHCASALDFPEPH
                     RELSMRVAGPDQIRVGDTEQFSALLENASTTSSVWGAVALFLDGFPKGTNVSSSPSII
                     PFHVPAGGRVSLGSELRVDTPTIPAHWYRAKAIAFLDGHWFLSAKMLRVRR"
BASE COUNT          352 a          662 c          622 g          422 t
ORIGIN      
        1 ccctgtgcat cgccttgggg ggactggcag cctgcatcat ggccgaatgc ttcgcgttcc
       61 accgcggcaa gcaactgctg agcctaaccg cccaagacct ctccacacaa ctgacggagc
      121 tcgaagagca actgttcagt catcgggatt ggattcaaag cgttactttg ttgtttccga
      181 gctgggcgaa ttcaggcgcg ggcccgaaca cacaagcctt ggagcgggtg ctaactctgc
      241 tgcgtaaggc cgagctcggc tacccggttg gcctactgac ggcacgcacc ccagcgctgt
      301 tgagtaccct gatcgtcaaa tcatgcgtac tcccgttgga actgggatcg attgctgtgg
      361 tcggcgcgca gtcggacgcc ccgcctatcc ctgcctcacg ggaggaccct gcgcgggaat
      421 tcttcactca agctgattta ctgcatcgga acgccgatcc gggactcgac tttcgagtgg
      481 aacacttgga cgagacgcgc tcgcccgcgg ccgtggtccg cgcgcttcac cacgagccaa
      541 tcggtacctt aattttccgg ggtcactcgc gcccgtattg cggcctcgaa ggccgcctat
      601 gcgcgtcccc ggcatcccag caacccacgc aagctgaatg cgtgctgggc ttggactgtt
      661 acgacgcatc ttggccaaag gtcgatccac gctcgtttgg cgcagaacta gttgtactcg
      721 agacctgctc cgctctgacc gcatccacca cggcatggga agacggcggg gcgcccatcg
      781 cgatcctggc gagcgaaagc cgcgctaccg ccgtactgac aaccgcggcc cccaccgtca
      841 tgcgagacgt cagcttcttg ctttcggctt tgacgcgcac tcgaacggtg ggcgaggcgg
      901 gagtcctctt cgatcaaagc gcccgttcac atcagggctg cccctcggta ttcttgctcg
      961 gcgatcctga ccagcctttg cccaactgct tcttcggcgc ggtgcccgag atcgggcgcg
     1021 aacacgacgc cggtgccgtc gcagctctgg gaggcagcga tacattgctt ccgaggccca
     1081 tttaccaacg gtacgcgctg ccaggagttc gcgaaaagac caccgtccgc gttaccggcg
     1141 acttgaacga cgtagagcag gcctcattgt gcctcatccc gggagcgtcg cacagtgagc
     1201 tgtggctccg ttcccgtcga aggaatctcg acgtttgcat ctcgccgacg ccagttccga
     1261 ggtttccaac agaagtattg tcagccgcgg tggcgctgcc cgcgcgtttg ctgtcgttca
     1321 cggggcttgc gtcggccggc gtacccggcc tgttgcaggc ttgccggagc gtcgttcagt
     1381 gtcaggcgca tctcgaatcc ttggcagacc gcgccaccgc gtacgatttc gaaggctacg
     1441 tcagaagtac gatcctggac tggtgggccg cgcatcgtga cttcttggaa tcaacactga
     1501 ctcagcgggg ccagtccttc tggatcgata atgtgtgctc cccaacggga ccaacgagcc
     1561 gccatagcac ctgcttcccg tgcgcccagt gtgggcgagc ttcggttacg attagaagtt
     1621 atgacgtttt tcccgccggt gcacggcagg cacgcgagtg cgagcactgc gcctctgcgc
     1681 tcgatttccc cgagccgcac cgcgagctga gcatgcgagt cgcaggtccg gatcagatcc
     1741 gcgttggaga cacggagcag ttctccgcgt tgttggagaa tgcgtcgacg acatctagtg
     1801 tttggggtgc ggtcgcgttg ttcctcgatg ggttccccaa gggcacgaac gtcagctcta
     1861 gcccgagcat catcccgttt cacgtccccg ctggtggccg cgtgagcttg ggctccgagc
     1921 tccgcgttga cacacccacg atccccgcgc actggtatcg ggccaaggcg atcgcttttc
     1981 tggatggtca ctggtttctc tcagcgaaga tgctgcgggt tcggcgttag agcgggggtc
     2041 cgccgaggcg ctctatcg
//
LOCUS       DASTKU010000543         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_773611, whole genome shotgun sequence.
ACCESSION   DASTKU010000543 DASTKU010000000
VERSION     DASTKU010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773611"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFQ61_18900"
     CDS             complement(<1..572)
                     /locus_tag="VFQ61_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956582.1"
                     /translation="MFRFSSRPFLAVLPLSLLTLLSGACSSTDNPSSGQSGGSSNNSS
                     SGGAAGSGAGKPGSGGASGGSTTSGSGGSGTNNSSGGSAGSGQASGGSANGGSASGGA
                     NSGGAQGGSNTATSGGSNSGGSTASSGGRTAGGTSAGGSSSGGASGNSGGSGGAATGG
                     AKGNAILTEGFDGPALPTGWDNFIGYVKNG"
     gene            931..2229
                     /locus_tag="VFQ61_18905"
     CDS             931..2229
                     /locus_tag="VFQ61_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956583.1"
                     /translation="MKSLLQSAAILVFLSVGCGNDSGGSAGNGGSPASGGARSGGAAN
                     SGGTNASGGATSSSGGAATSGGTTSSSGGTTSSSGGSNTSGGKASGGASSGGAPASGG
                     SASGGRESTGGAGGGQASGGRASGGAGGQGTAGTSSSGGAGGGAAGGGSTQSAGCGKA
                     PTIMSSQYNNGTTIPITVGGVARRYVLNVPKNYDMNKPYKLVVTLHARDGNDKRMYNW
                     NYYGLLPLSNDTAIFVAPNGSQTNNQPCTGTGDCEASCGWPNSGGKDLALVDAVVAEM
                     EKNFCIDTTRIFATGWSYGGSMSYAVGCARPLGGTEASWGVRAIALYSAAQLSGNCTP
                     SMPVAFYASHGTKDSVLNYEQMGSLPLAQNFAKANGCTWATPTKVTSGNHVCSNMMGC
                     KMGYPLEYCSFNGDHTPFPDNGTASGSWGPQEAWKFLNQF"
     gene            2544..>2823
                     /locus_tag="VFQ61_18910"
     CDS             2544..>2823
                     /locus_tag="VFQ61_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956584.1"
                     /translation="MGTLDARSRIVALCCWLVACGRASGDDQAPSMARGGTAGEVGAA
                     GESASGHANAGMGVGARGGGGSGGIVDAGLSGGGGISGAAGGVGGGSNV"
BASE COUNT          518 a          851 c          978 g          476 t
ORIGIN      
        1 atgccgttct tgacgtaccc aatgaagttg tcccagccgg tcggcagtgc gggcccgtcg
       61 aacccctcgg tgaggatcgc gttacctttc gctccgcccg ttgcggctcc accgctccca
      121 ccactgttgc ccgatgcgcc ccctgaagag ctgccgccag cactcgtgcc gcccgcggtc
      181 ctgccaccgg acgacgcagt cgagcctccc gagttggagc cgccgctcgt agccgtgttc
      241 gaaccaccct gagcaccgcc cgaattggcg ccgccgctcg ccgacccgcc gttggccgag
      301 ccacccgacg cttgtccgga accagccgaa ccgccactcg agttgttcgt tccggaacct
      361 ccgctaccgc tcgtggtgga gccgccactc gcaccgcccg aaccgggttt gccggccccc
      421 gaccccgcgg cgccgccaga tgacgagttg ttcgagctac ctccgctttg gcccgagctc
      481 gggttgtcag tgctcgaaca agctccggag aggagggtca ggagagagag gggaagaacg
      541 gcgaggaaag ggcgcgagga aaagcgaaac acgcagcata tcaagcacgc gccttcagtt
      601 ttcgccagtg ccgcttcgcg tgagaaccaa gccaggaaca cgcgacagaa cccgcataac
      661 agcttcactg agaagtcgcg gtgcgcttgg aaaacgggtg tgctggactc ggcgcttcac
      721 tttcgcggag cgcacgtcgg acgtcgacag tgcggaaatt cggatcagtt gattcgtgaa
      781 tgcgtgcatc ggcgacgcgg acggcgagct cgggtagaat ttttcgatga gcgcgtcgtt
      841 cgagacgtgc gtggtagtga gcgcgagaaa aagtatcaag agatgatcat ttcttaacaa
      901 cccttacggg ctcggggcac tgctcgagtg atgaagtcgc ttctccagag tgctgcgatc
      961 ttggtgttcc tatcagttgg ctgtggcaac gactccggag gttcagccgg gaacggaggt
     1021 tctccagcga gcggcggggc acgtagcgga ggcgcagcga atagcggcgg tacgaacgcc
     1081 agcggcggcg ctacgagcag tagtggtggc gcggcaacga gcggtggcac cacgagcagc
     1141 agcggtggca ccaccagcag cagtggcggc tccaatacga gcggtggaaa agcgagtggt
     1201 ggcgcgtcga gcggcggtgc gccggcctcg ggcggaagcg cgagcggcgg tcgagagagc
     1261 acgggcggcg caggcggagg gcaggcgtcc ggcggtcgag catcgggcgg ggccggtggt
     1321 caaggcacgg cgggtacgtc cagctctggc ggcgcaggtg gcggcgcggc tggcggcggc
     1381 agcactcaga gcgccggctg cggcaaggcc cccaccatca tgagcagcca atacaacaac
     1441 ggcacgacca ttccgatcac ggtcggcgga gttgcacgtc gctacgttct caacgttccg
     1501 aagaactacg acatgaacaa gccgtacaag ctcgtcgtca ctctccacgc ccgggatgga
     1561 aacgacaagc gtatgtacaa ctggaactac tacgggctgc tcccgctctc taacgacacg
     1621 gccatcttcg ttgcgccgaa cggctcgcaa acgaataacc agccgtgtac cggcaccggc
     1681 gactgtgaag cgagctgcgg ctggcccaac agtggaggga aggatctcgc cctcgtagac
     1741 gccgtcgttg cagaaatgga gaagaacttc tgcatcgaca cgacgcgcat ctttgcgacc
     1801 ggttggagtt acggcggtag catgtcttac gccgtcggct gcgcccgccc cctcggtggt
     1861 accgaagctt cttggggtgt gcgagcgatt gctctctatt cggcggccca gctgagcggc
     1921 aactgcactc cgagcatgcc cgttgcgttc tatgcgtcgc acggcaccaa ggattcagtt
     1981 ttgaactacg agcaaatggg aagtctcccc ttggcgcaga actttgcgaa agccaatggt
     2041 tgcacctggg cgactccgac caaggtcacg agcggaaacc acgtgtgctc gaacatgatg
     2101 ggctgcaaga tgggctaccc gctcgaatat tgctccttca acggcgatca cacgccgttc
     2161 cccgacaacg ggaccgcctc gggtagctgg ggcccgcaag aggcctggaa gttcctgaat
     2221 cagttctgag cgaccacgct cggagggcag ctcgcgactc agaacgcggt tgagcgtaac
     2281 tgagtcgcgt ggctgcgcgg cggcggttcg gcttcttcac gggggggcgc ggtagctcgc
     2341 gagtcggcgt gtgactggtc gcgatggcgt cgcggtgcca cggagacgcg tcgagggacg
     2401 ccgctgacgg atccgattgg gtgatggatc gagcgtcttc ggcgcactgc cgatgacgtt
     2461 ggggtcgacc cacgttttgc gcgagccttg gtggaaagct cgaggcttcc ccgtgcaaaa
     2521 gccgaagcgg gaagaacaag actttgggta ctttggatgc tcgttcgcgc atcgtcgcgc
     2581 tctgctgctg gctcgtggcg tgcggtcgcg cttcgggcga tgaccaggcg ccgtccatgg
     2641 ctcgaggagg gacggctgga gaagtgggcg cggctggtga gagcgcgagt gggcacgcga
     2701 acgcgggtat gggtgtcggc gcgcgcggcg gaggcggcag tggcggcatc gtcgatgcgg
     2761 gcctcagcgg aggcggtggc atttcgggcg ccgctggtgg cgtcggaggc gggtccaacg
     2821 tct
//
LOCUS       DASTKU010000544         3454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_552078, whole genome shotgun sequence.
ACCESSION   DASTKU010000544 DASTKU010000000
VERSION     DASTKU010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552078"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VFQ61_18915"
     CDS             <1..925
                     /locus_tag="VFQ61_18915"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9956585.1"
                     /translation="EPTEIFGPINLRKLKEGVVETEIGGMLPEAEVAFLDEIFLGSTA
                     ILNTLLGILNERSFRRGHTHITCPLRVCVGASNRLPEDDQLAAFADRFLVRLFVEPIG
                     DSGLEELLSSGWSLGSKLNPVTSSMAHLDTLGHAALEMDLTSVRGALAHAIRLLRGAG
                     VELTDRRIVRLQRLVAGAAALAGRTQPTIADLWPIVFALPTQPAQQLGREVLRELLAG
                     SENQALVSATAEGSLGARARGARILRDGTAILNDAPPDEANRGAWLLRVEGIAREIDA
                     TFTRDSLPADLADLRARIVDKLAASAAAANR"
     gene            952..1392
                     /locus_tag="VFQ61_18920"
     CDS             952..1392
                     /locus_tag="VFQ61_18920"
                     /inference="COORDINATES: protein motif:HMM:NF040397.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956586.1"
                     /translation="MQEINGLQLAFLPREEQLEPCAAVASGSAARALAERVLRAHDDQ
                     LRTWSGVAADGLFIVLGDNDSLPWVEGIRYLGRDPSAPRLLLPTNLRPALVALEVFER
                     ALFSHAPRLGAPLAVLLDPPRVVSVSDALPIRRDRVSAWLEANS"
     gene            1371..3191
                     /locus_tag="VFQ61_18925"
     CDS             1371..3191
                     /locus_tag="VFQ61_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956587.1"
                     /translation="MARGELVIDLPRHLSQWGPQLALFPKELALALGPLVSRLANLVG
                     GWRVAETHDGDPDGYDGIARRGTYDRLLPTEWLLLDEIPEEFVRRVVAGEHAFLRRAQ
                     RASVGERRCLALFDAGSDQLGTPRLAHLALLVLLARRADEQRATLEWGVLQDPELALR
                     TGLTRSSVLHLVRAGCGRTVSPSDFDRWVEVAQRAKGAEIWLIGANAVAPQASRFHAS
                     VVLVSDVIEPNPPQRIRVRVSDSRAAVSRADVSFSKEVILDVPSDPLAVQLLRDPFAA
                     RGASRQVAAWRVDVNSNLVFAADGRRLYARGAASTLVTLQVPNSPHATPGPTTAFNAP
                     DGHTILAAGRLFSRKRTVVVSQLGQELFLHVLSRSGSSATQSLRFTPSSGYTLPTPSA
                     TRLSPVGVLEPNRFYFLDPQGRVVELVDGSVQPRENFRSIAARALRDCFAWVVAKAGS
                     PQILWVRTNKEGQVEMHQSLLPGANVSLDSASFRFGVGVSEAYAYMTALHGWVIANER
                     SCTRVSVPEQAQVVGLVKLQAGRPEVGLLVLDASDTRIQVLQSGELKTLFTSGAGIAG
                     VEVSGTGLDVAYLTESGELGVYSCTVNAIVARLQAEMLDE"
     gene            3184..>3454
                     /locus_tag="VFQ61_18930"
     CDS             3184..>3454
                     /locus_tag="VFQ61_18930"
                     /inference="COORDINATES: protein motif:HMM:NF039753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bpX6 domain-containing protein"
                     /protein_id="HET9956588.1"
                     /translation="MSEVRLRRPIHHGSVLAAGLAIDTRLVPEPIARRRILELSSVVQ
                     QVRRAEAQLITVFRDPVRMSCASALGTPLVRQGRLLSAAPLERDEQ"
BASE COUNT          573 a         1080 c         1129 g          672 t
ORIGIN      
        1 ggagccgacg gagatctttg gtccaatcaa tctgcgcaag ttgaaagaag gagtggtcga
       61 gaccgagatc ggaggcatgt tgcccgaagc ggaagtggcg tttttggacg agatcttttt
      121 aggctcgacc gcgatcctca atactttgct cggtattctc aacgagcgct cgttcaggcg
      181 aggtcatact catatcacct gtccccttcg cgtctgcgtg ggcgcatcga atcgcttgcc
      241 tgaagacgat cagctggcgg cgttcgcgga tcgcttcttg gtgcgcttgt tcgtggagcc
      301 cattggggac tccggactcg aagagctact ttcatccgga tggtcgctgg gctcgaagct
      361 gaacccggta acgtcatcga tggcccacct ggacacgcta gggcatgccg cgttggagat
      421 ggatctgacg agtgtgcgcg gtgctctcgc gcacgcgatt cggctgttgc gaggtgctgg
      481 agtcgagctc acggatcggc gcatcgtgcg tttgcagcgc ttggtcgccg gcgcggctgc
      541 gctcgcgggc cgtacccaac ccacgattgc ggatctgtgg ccgatcgtct tcgcgctccc
      601 tacccaaccg gcgcaacagc tcgggcgcga ggtattacgt gagctgctgg cgggctccga
      661 gaaccaagcc ttggtttcgg ccaccgcgga aggctctttg ggagcgcgcg cccgaggggc
      721 acgcatccta cgcgacggga cagcgatctt gaatgacgcg ccccccgatg aagcgaaccg
      781 cggcgcgtgg ttgctccgcg tggagggcat cgctcgagag atagacgcga cattcacccg
      841 cgactcattg cccgcagact tggcggacct gcgagcacga atcgtcgaca agctcgccgc
      901 gtccgcggcg gccgcaaacc gctgagtgga gctctctccg ggggcggcac gatgcaagag
      961 atcaacggct tacaactagc tttcttaccg cgcgaggagc aactcgagcc ttgcgcggcg
     1021 gtggccagtg gcagcgcggc gcgcgcgctc gccgagcggg tgttgcgcgc ccacgacgac
     1081 cagcttagaa cttggtcggg ggttgccgct gacggcctct tcattgtctt gggcgacaac
     1141 gattcgctgc cgtgggtgga aggaattcgc tacttgggtc gcgatcccag cgccccacgg
     1201 ctcttgcttc ccacgaactt gcgacccgca ctggttgcgc tcgaggtgtt cgagcgagct
     1261 ctatttagcc atgctccaag gctcggcgcg ccgctcgcgg tgctgctgga tccgcctcgc
     1321 gtcgtatccg tgtcagacgc gcttccgatc cgacgggatc gggtgagtgc gtggctcgag
     1381 gcgaactcgt gatcgatctg ccgcgccatc tttcgcaatg gggcccgcaa ttggcgctct
     1441 ttccgaaaga gctagcactc gcgttgggac cgctcgtctc tcgcttggcc aatttggtcg
     1501 gaggctggcg agtggcggag acgcacgacg gtgacccgga cggctatgat ggtattgcac
     1561 gacgaggtac ctacgatcga ctcctgccca cggagtggct gctgctggac gagatacccg
     1621 aggagttcgt gcggcgcgtg gtggccggcg aacatgcctt tctccggagg gctcagcgcg
     1681 ccagcgtcgg ggagaggcgc tgcctagcac tgttcgatgc agggagcgat cagctgggca
     1741 ccccccggct cgctcacctc gcgttgctgg tgctgctcgc gcgccgcgcc gacgagcagc
     1801 gcgcaaccct ggaatgggga gttctccaag accccgagct tgcgcttcga accgggctga
     1861 cgcgctcctc ggtactccac ttggtgcgcg ccggctgcgg gcgcacggtg tctccgagcg
     1921 atttcgatcg ctgggtggag gtcgcgcagc gcgccaaagg cgcggaaatc tggctcatcg
     1981 gagcgaacgc ggtcgcgccg caagcctccc gttttcacgc gtcggttgtc ttggtgtccg
     2041 atgtgatcga gccgaacccg ccccagcgaa ttcgtgtccg cgtctcggat tcacgcgcgg
     2101 ccgtatcgag agctgacgtc agcttcagca aagaggtgat tctggacgtc ccgtcggacc
     2161 cactcgcggt gcagttgctc cgcgatccgt tcgcggcgcg gggcgcatcc aggcaggtcg
     2221 ccgcttggcg agtcgacgtg aattcgaacc tggtattcgc ggccgacggc agacgactgt
     2281 acgctcgtgg cgctgcctct acgctcgtca cgctgcaggt tccgaattcc ccgcacgcca
     2341 ctccagggcc cacgacggct ttcaacgctc cggacgggca cacgatcctc gccgcaggtc
     2401 gattgttctc caggaagaga accgtcgtag tctcccagct tggacaggag ctctttcttc
     2461 acgtgctgtc cagaagcgga agctctgcca cgcaaagcct ccgcttcacc ccctcgagcg
     2521 gctataccct gcccacaccg agcgccacac gacttagtcc agtcggagtg ctcgagccga
     2581 accggttcta ctttctcgat ccgcaaggcc gagtagtgga gttggtcgac ggctccgtcc
     2641 agccgaggga gaactttcgg agcatcgccg cgcgagcgct ccgcgactgt tttgcctggg
     2701 ttgtggccaa ggcgggatca ccgcaaattc tctgggttcg gacgaacaag gaggggcagg
     2761 tcgaaatgca tcagtcgttg ctcccgggag caaatgtgag cctcgattca gccagctttc
     2821 gattcggcgt cggggtctcc gaagcttacg cctacatgac ggccctccac ggctgggtca
     2881 tcgcgaatga gcgttcctgc actcgagtct cggttccaga gcaagcgcag gtcgtcgggc
     2941 tcgtcaaact tcaggccggt cgccccgagg tagggctgct cgtgttggac gcaagcgata
     3001 cccgaatcca agtcctgcag agtggagagc tcaagactct atttacgtcc ggtgcgggca
     3061 ttgccggcgt cgaagtcagt ggcacgggtt tggatgtggc gtatctcact gaatcggggg
     3121 agctgggcgt gtactcctgc acggtcaacg ccatagtcgc tcgactacaa gcggagatgc
     3181 tcgatgagtg aagtgcggtt gcgtcggcca atccatcatg gctcagtgct cgccgccggt
     3241 ctcgccatcg acacgcgact cgtgcccgag cccatcgcgc ggcggagaat actcgaactc
     3301 tcgtcggtgg tgcagcaggt gaggcgcgcg gaagcacagc tcatcaccgt gttccgcgat
     3361 ccggttcgaa tgagctgtgc ctccgcactc gggacgccgc tcgtacgtca gggccgtctg
     3421 ctgtccgcgg cgccgctcga acgcgacgaa caga
//
LOCUS       DASTKU010000545        14735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_552165, whole genome shotgun sequence.
ACCESSION   DASTKU010000545 DASTKU010000000
VERSION     DASTKU010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14735
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552165"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1944)
                     /locus_tag="VFQ61_18935"
     CDS             complement(<1..1944)
                     /locus_tag="VFQ61_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956589.1"
                     /translation="MTDSDVKILWTASEAEYAQAVATSTLHVPVIRDPADPAPGGFGD
                     SKWLDYAQWAEQTPQASSSPTRVPPSPDRLCVALGSGVSESAARLVAERTGRSWLRAR
                     SASELEAWLRQPGAAATRRSLTWFCPAFDGHRGGANPSLLREVLAFMRSLAERGVAVP
                     WGIITGINAAALTRLAAKAILQHRITESYRARSALVVNFFDPPALKSPIPPYELGARP
                     DQPLLRVDEASLQPGHASQIVRESWSLISFKAHGRSYCAARGFLCAARTPGERLDAAP
                     KQCVLGMQCLSSRFPQIDPRRFDTPLLVLDACGSGNLATRVWETGQPSIAYLAAAGAP
                     SAIVTGDTVTMNQAGDFVDILWSLQSASTLGEAVQRLNSVRREPDGAVKLTYFLLGDP
                     EIGAGLERFPEFAHRAAAVDAARPGGQRAPYLEVRKNDDAPSSGSDGALHLWTEDPKS
                     RVVGERLFRFDNTSTVWFGWSEGPSRAPVPDVFVQRAARLELPHGLLEAACDMPRRTL
                     AWTPPLQASGRSLVESADKVIRVGRLVEHSKGAVVTNRPQDVALAVRVAQQGWLQAHI
                     ACIKAMLALADGGIWPFRFWSAQDFTRRELACACKYCGVTPSLERRYRSYPTDFRLQR
                     ECLDCDLIDDRPEGYGFPNVNFDV"
     gene            complement(1941..7751)
                     /locus_tag="VFQ61_18940"
     CDS             complement(1941..7751)
                     /locus_tag="VFQ61_18940"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9956590.1"
                     /translation="MRAAVTEASLDVTPTEASSGKEYRLSPQQRRTWRQQPSDAWLTA
                     GVRLRLARRPSAQALQRALSGLGQRHPVLRYAFANAGATEAEPSLPRAVLCGALQCRP
                     RSSSEAAERHPAVRQVSSSSRGPRSTSGNQVSYSLSTGSAGTTELLVESPALNADSKT
                     WLALAAELGSALSQVAPPSTPDGPDFGLAAEWQNQLLDDGASNAESPQPADPRLAFEQ
                     HRGEFAIGQHPVVIDVDLSSRLEALSDTHAFSFESALLLAFSLVLARVSGEAQFRVGV
                     LRDERWYAELEHCLGPLSAVAPVTVSVWTEGSLFDNWARLALQLEASRSEQSPSLWQC
                     DFEPDLRGQDPLDRDRAPCAAQFEFIQVPQPRIGADAFQIEEVFNHLETFKLKLSCTR
                     TPGGLRATLFYDACSVLADEAQLFSERIVTLLEQLLEEPSLPCHAANCLGPREREILM
                     RGGLPDNGHEPADLVSWFERVALRYPNRVAVQCGSDALTYRELDARATHIAGALQRRG
                     IGPESRVALCMDRSVELIASLLGVVKAGAAYVPLDPQHPDERLCLLTERTRAALVIAD
                     STHVTRFNGPSLATYADLLADPSAARIAATHGRTALYVIHTSGSTGVPNGVIVERDQM
                     LSYLLALQKVLPLPELRSWASVTTVASDLGNTAIFGALCFGATLHLVPKQLVLDAVAF
                     GRYVRENGIEALKITPSHLSALLDADPGGAALPERFLVLGGESTSWELFDRVSQVRPR
                     CTVVNHYGPTETTVGVIAQRVQAEPRGWSSTVPLGIALRRASAHVLDDALRPALIGVA
                     GELYLGGPQVSRGYFGNPRKTAASFVPDPFSQRSGRLYRTGDRARVLPDGSVEFLGRR
                     DQQLKIRGYRVELNEVAASLRRQPRVRDALVRVSGEGTQRRLIGYVVPSKLDATLEGQ
                     LVDARVDEWRQVFAETHAALARQAATTFTTHGWVSSYTQQELPPAEIQEQVDAITQRV
                     LSWHPETVLEVGCGTGLLLTQIAEHCSRYVGTDFAPELLAFVRRTLADAGPRFAHVQL
                     MEQPAHTTETLPDAAFDVIVLNSVIQYFPSLDYLRRVLAGLAPKLRPGGRIFLGDVRS
                     FPLLEAFHASVQVARAESGTSLATLAEAVEQKCRLEGELVIAPRWFQSLTETIPELGV
                     VEARLKRGHFHNELTCYRYDVVLHSQQACRTRAAEVEHTWGEQLRSPDDLERWLGHTP
                     VDCVWIRNLPNRRVVVACALGRALSARDPRATVDDLRSAAANVGRDAYDPEDFIAAAA
                     RAGFDARAHWGSEAHCFDVSCVRRSVLADGTSELVVLSPSADVPIEHEASNDPLFERY
                     SRVLAADLKRALAAELPDYMVPSALVFLRRFTLTENGKIDTSGLPEPAQFHRHSREYV
                     PPRSETEAKIAAIWAEVLALSQVGAEDDFFELGGHSLLATQVVARMRSAFQAELPLSA
                     FFEGNPTPAKLAKVIEALRRDRTLDAGEPEPQELRLSRRPTGVSRVPTGFAQERILRG
                     PDPEHPAFNVPFAFYLDGALDERALLEGIRCIIARHEALRTRILSTDAGFLQEISEQP
                     GFDLEWIDLSQEPEPKQRAIAQQLAQSRGKGRFDLANGPLIRVVLLRLGNARHALILS
                     VHHVVSDGWSTGLFCRELSELYTAASHGSAARLPALAVNYADYCAWQRKQLNEAELAR
                     LHRFWSAELAGSSFALDIAPGRARPNPQNYCGAGLDVALPKALSAGLQQLAQASGATL
                     FMTLLALYDVVLAKLSGQSDFVVGTPIANRRREELEPLLGCFINCLVLRCRIEAGLSF
                     RQLLEGVKQTCLRAYEHQDYPFSELARAVQDGRGPAPKQALDPARPPLFQVMFELFNT
                     PTQTALKLGDLRLSPLEFVHGTSEFELNLILREGADGLRGWLLYRTHLFSEQEARWIV
                     SSLEHVARQVLAHPDAPLASLELAPTRSHAAEAERS"
     gene            complement(7736..14710)
                     /locus_tag="VFQ61_18945"
     CDS             complement(7736..14710)
                     /locus_tag="VFQ61_18945"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9956591.1"
                     /translation="MKPLSAERVLALDPARRELLERRLRARLAGGGPRLVRRGPGDAP
                     LSFAQERLWFLHQLGSTGSAYHVPICLEFDGELDVAALRLTLQALQGRHQVLSSAVRE
                     RRGEPFQTVVADAPLRFEHVRNADPGRVAALLAEFAARPFDLAEAPLFRSLLLETGDR
                     SGVLLLVFHHLVFDGWSIDVLLQEFAAQYAARRSGTRSEPAELAIQYADYCFWQRAQA
                     SDTELARRAEAWHVRQGGTAPPHALRLRSQGTGSSNAPCGSSTKVVHIQPLSADLRRR
                     LDHFARERSATRFVVSLTAFSLALRAESGIDDIGLLTPLANRGLPDVAPLIGCFANTG
                     LVRADLSGVITSTDALTRVQRAVLQAQEFEDVPFHILASRLQGERGTTLNPMACVAFS
                     VQNFARRFELEGARFSVVDVFAPDVAIELNATLRDTADGAELVIEFSRSRLELASVRD
                     LAIRFVACLERLVTHADAPLGEPPAVLRERECKPSRAQMLMWLGQARYPELPLYNQQA
                     VLELEGELDVGRLQNAFSRLVAVTDILQTRFITRDDGLRQVFGGARFGTLPLVPADPA
                     RLEEISKERATLSLCNREPHWDAVLLETARGRHHLVLTIHHLLIDGHALGELFRRLSE
                     IYAGTDRAENYPGFEAYLERDARKHEEGGYCSSATFWKQKLSTKPTPLEFYGHKRGPE
                     TTEIHSRILPLSSARLGRLIDSGRSNGVAGFSRLQTLANVLLIGLLATLRRATGVEEH
                     SLGVTLANRAGPEERRIVGPLMSVGVLRSQVDAGRDFVALGRQLLQASAEMMRHATYV
                     VDNPAEERVYDVLFNLVSLESPSFEGCSATLRLSNGSHEYDRVALHAEWSNGELTLKF
                     NAQKHVMGEAELARFCADFERALEQYETAPHSPLSLFSRGDWSEASLGGAPALVGAPG
                     GLLDQIEFDSRTLDRIAIEHAGQALTYAELWASVDALSSQLAEALDAPEQPVIHLLPR
                     RLQAITAILAILRAGGVYVPIDPATPVARVRELVQRMGARAAVAEREWAPLLSDLGLH
                     VITPEPEAPKKPLTSSSRAWSPKAAYSAGELAYVLFTSGSSGRPKAVAMSHGALHHFA
                     LAARQYMQLGPTDRVLQFANLAFDASLEEIFATLTAGATLVLRDESMLTGSLSFLEFV
                     ARERISVLDLPTALWNLLGRELSRIGSLPDLSRLRSIVIGGEAADHEALVGWRRRLPS
                     HVQILNSYGPTECCVVSAVHVLSARDEVRGSIPLGKPLPGTTISILDAAGDPVPRGVC
                     GEIYIAGSGLARGYWRDPGESAKRFVPAPQGPPGRLYRTGDRGRINDEGLLEFRGRQD
                     RQVKVRGQRVELAEIEAQLVRLQGVRQAAVRVGGRDAAGSCTLVAIVERTPSSTLVPQ
                     RVAAQLRERLPSYMVPAEIRIVDRMPLNAAGKVDHAALAEQSRHSAHSEAANERALSG
                     EAEHQLARIWAELLKISPEQIGAEDSFFDLGGHSLLGTLLVSRIEETFGVRLPLSALF
                     EAPELASLAERVSHSRPSVAPSAGIPRRAGARFPLSSEQRRLWFLYQLKPEGTEYNVT
                     AAVRIRGSFRAEIAIQALRAIVQRHEVLRSVITMEGGVPTQCVLPNAALKVSRSACAE
                     LPESTLIQAIVRAAQEESQRPFRLETGPLLRARLLEFGAENHALIVSAHHIVCDGWSV
                     GLLVNELTRTYEAALAGSLPELPELAIQYGDYAAWQADALAPEERRAQLEYWQRNLAD
                     LPPPIRFPLPTAPGRVNTGVTHFAAIPEDLANAVTRVAGEAGITPYMLLIAVWGVLLH
                     RWTGSDDLLIASPIAERTRPETHNLIGFFVNTLVLRLRIGRSFSIHDVLKQARSVVLN
                     AFNHRDVPFDSVYETLNLPRGEASSALFRVMFTYLNEPTRRLLEPTSIDWEAVEFDRG
                     AANRDLTLRIEEASGQLRAHLEHDASKISEACGRDLLAYYVDVLRYAVDHLHARVSDV
                     PLPQSLNPTRSIPEPLPSRRAGLQSILKRGQGSPPSTEQLVSTRNYWPDDPLILLVTP
                     RAPGVNLADWIREQRQLVQQRLSEQGAILFRGFDVSDAADFEALCLAMSPRLEEYQQA
                     STPRSRVRERIYTSTEYPASESIMLHNELSYSHCWPGKIWFYCHTPPAAQGATPLASG
                     RALLARLDREVRDAFERKGVMYQRNYGSGLDVPWHEVFGTQDRDRVESYCRQHDIQWS
                     WLGGDRLRTLEVHKALLRHEHTGEPVWFNSAHMFHVRAHQQATYASLRELFADSDLPR
                     HAYYGDGSPIPDAYIEHIRDSYRAVARRFDWRRRDVMLVDNVLVAHGREPFSGPRKIL
                     VSMAEPQTERDGRSCAQQ"
BASE COUNT         2666 a         4827 c         4751 g         2491 t
ORIGIN      
        1 gacgtcgaag ttcacgttgg ggaaaccgta cccctctggc cgatcgtcga tcagatcgca
       61 gtcgaggcat tcgcgctgca aacggaagtc cgttggatag gagcgatagc ggcgctctag
      121 gctcggggtg actccgcagt acttgcaagc gcaggccagt tcgcgacgcg tgaagtcctg
      181 ggcggaccag aatctgaatg gccaaatgcc gccgtcggcc agcgcaagca tggctttgat
      241 gcatgcgatg tgtgcttgta accaaccttg ctgggctacg cgcaccgcga gcgcaacgtc
      301 ctgggggcga ttggtgacca cagcgccttt ggagtgctcc accagacggc ccacgcgaat
      361 cactttgtcg gcactctcga ccaacgaacg gccactcgct tgcaggggtg gcgtccaggc
      421 gagagtccgc cgtggcatgt cgcaggcggc ctccagcaga ccgtgcggaa gttcgaggcg
      481 cgcggcacgt tggacgaata catcagggac gggagcgcga ctaggtccct cggaccaacc
      541 gaaccagacg gtggaggtgt tgtcgaagcg gaacaatcgc tcacccacca cgcgactttt
      601 cgggtcttcg gtccaaagat gcaaggcgcc gtctgatccc gaactcggtg cgtcatcgtt
      661 ctttcgcact tctaggtagg gagcgcgttg gccgccaggg cgcgcggcat cgacggcggc
      721 cgcccggtgc gcaaactccg gaaagcgctc caagcccgcg ccgatttctg ggtcgccgag
      781 caagaagtag gtgagcttga ccgctccatc gggttcccgg cgcacgctgt tcaatcgctg
      841 cactgcctcc cccaacgtcg acgcgctctg gagcgaccac aggatgtcca cgaagtcccc
      901 ggcttggttc atcgtaaccg tgtcgcccgt gacgatcgcc gaaggcgcgc ccgccgccgc
      961 caggtaggcg attgagggtt gaccggtctc ccacactcgt gtcgcgaggt ttccgctgcc
     1021 gcacgcatcc aacaccagca aaggggtgtc gaagcgacgc ggatcgatct gcggaaagcg
     1081 gctgctcaga cactgcatcc cgagcacgca ttgcttcggt gctgcgtcca gccgctcccc
     1141 cggagtacgc gccgcgcaca agaagccacg cgccgcgcag tagcttcttc cgtgcgcctt
     1201 gaatgaaatc aaactccagc tctcgcgaac gatctgcgat gcatgtccgg gctgcagcga
     1261 cgcctcgtcg acgcgtagca ggggctgatc ggggcgcgct cccaactcgt aaggggggat
     1321 gggagacttg agcgcgggtg gatcgaaaaa gttgaccacc aacgccgatc gcgcgcggta
     1381 gctctcggtg atgcgatgct gcaggatcgc cttggcggcg agtcgcgtca gcgccgcggc
     1441 gttgatgccc gtaatgatac cccacggaac cgcgactccg cgctcggcca ggctgcgcat
     1501 gaacgccaac acctcacgca acagcgacgg gttggcgcca ccgcggtgcc cgtcgaaggc
     1561 cgggcagaac caagtcaggc ttcgacgcgt ggctgcggcg cctggttggc gtagccacgc
     1621 ttccagctcc gacgcggagc gggctcggag ccagctgcga cccgtgcgct cggccaccaa
     1681 acgagccgcg ctctcactca ccccggagcc gagggcgacg cagagccgat ccgggctggg
     1741 cggcacgcgc gtcggcgagg aggacgcttg gggcgtctgc tccgcccatt gcgcgtagtc
     1801 gagccacttc gaatcaccaa agccgccggg cgctgggtca gcgggatctc ggatgacggg
     1861 cacatggagc gtcgacgtgg caaccgcctg ggcatattcg gcttccgacg ccgtccacag
     1921 gattttcaca tccgagtcag tcatgatcgt tcggcctccg ccgcgtgaga gcgggttggg
     1981 gcgagctcca aggatgcgag cggcgcgtcg ggatgcgcca acacttggcg ggccacgtgc
     2041 tcgagcgagc tcacgatcca gcgggcctcc tgctccgaga acaagtgagt tctgtacaac
     2101 agccagcctc gcagtccgtc ggccccttcc cgtaagatga ggttcaactc gaactcactc
     2161 gtaccgtgga cgaactcgag cggactcaac cgcagatcac cgagcttcaa ggccgtttgc
     2221 gtcggggtgt tgaatagctc gaacatcacc tgaaacaggg gcggtcgcgc cggatccaag
     2281 gcttgcttcg gggccggacc gcggccgtcc tgcaccgcgc gcgcgagctc ggagaacggg
     2341 tagtcctggt gttcgtaagc acgcaaacac gtctgcttga ctccctccag cagctggcga
     2401 aacgaaagcc ccgcctcgat tcggcaacgc agcaccaggc agttgatgaa acaaccgagc
     2461 aggggctcga gttcctcgcg tcgacggttg gcgatcggcg ttccgacgac gaagtcggat
     2521 tgcccggaga gcttggcgag cacgacgtcg tagagcgcaa gcaaggtcat gaacagcgtc
     2581 gcgccgctgg cttgcgcgag ctgctgtaag ccggcgctca gcgccttcgg cagggctaca
     2641 tccagccctg ccccgcaata gttctgcggg ttcgggcgcg cgcgccctgg agcgatgtcg
     2701 agcgcgaagc tcgaccccgc cagctcggcg gaccaaaacc ggtgaaggcg cgcgagttcg
     2761 gcttcgttca gctgcttgcg ttgccaggcg cagtaatctg cataattcac cgcgagcgcg
     2821 ggcagccgcg ccgccgatcc gtggcttgcg gcggtgtaga gctcgctcag ctctcgacag
     2881 aacaagcccg tggaccagcc atccgagacg acgtgatgaa ccgacaagat gagagcgtgt
     2941 cttgcgttgc cgagacgcag caacaccact cggatgagag ggccgttcgc caggtcgaaa
     3001 cgtcctttgc cccggctctg ggccaactgc tgcgcgatcg cgcgttgctt gggttcgggc
     3061 tcctggctca ggtcgatcca ctcgagatcg aatcctggct gctcggatat ttcctggaga
     3121 aacccagcgt cggtcgagag gatgcgggtg cgtagcgctt catggcgcgc gatgatgcaa
     3181 cgaatacctt cgagcagggc tcgttcgtcg agcgctccgt ccagatagaa tgcaaacggg
     3241 acgttgaagg cgggatgctc tggatcgggg ccacgcagga tgcgctcttg cgcgaagccc
     3301 gtcgggactc ggctgacgcc ggttggcctt cgtgaaagcc gcagctcttg cggctcgggt
     3361 tcccccgcgt ctaacgtgcg gtctcgacgc agggcttcga tgacttttgc caacttagcc
     3421 ggggtcgggt tgccttcgaa gaaagcgctg agaggcagct ccgcttggaa tgccgatcgc
     3481 atgcgcgcca cgacctgcgt cgctaacaac gagtgcccac cgagttcgaa aaagtcgtct
     3541 tccgctccca cctgtgagag cgccaagacc tcggcccaaa tcgcagcaat cttggcttcc
     3601 gtctcgctcc ggggtggcac gtactcgcgt gagtgccggt gaaactgtgc cggctccggc
     3661 agcccggagg tgtcgatctt gccgttctcc gtcagggtga accgtctcaa gaacacgagc
     3721 gcgctcggca ccatgtagtc ggggagttcg gcggccaagg ctcgcttcaa gtcagccgcg
     3781 agcacacgcg agtaacgctc gaacagcgga tcgttgctcg cttcatgctc gatggggacg
     3841 tcggcggagg ggctaagcac aacgagttcg ctagtgccgt ccgcgagcac ggatcgacgc
     3901 acgcagctca cgtcgaagca gtgggcctcg ctcccccagt gcgcgcgcgc gtcgaagccg
     3961 gctcgggccg ccgcagcgat gaaatcctct ggatcgtagg cgtcgcgtcc aacgttcgcg
     4021 gcggcggatc tcaagtcgtc gacggtcgcc cgcggatcgc gcgccgatag cgcgcgtccg
     4081 agagcgcaag ccacgacgac acgccgattc ggcaggtttc ggatccaaac gcaatctacg
     4141 ggagtgtggc ccagccaccg ctccagatcg tccgggctgc gcaattgctc gccccaggtg
     4201 tgctccacct ccgcggcccg ggtacggcac gcctgctgac tgtgcagcac gacgtcgtac
     4261 cgatagcacg tgagctcgtt gtgaaagtgg ccacgcttca gacgggcctc gaccaccccg
     4321 agctcgggga tggtctcggt cagcgactgg aaccagcgcg gggcgatcac cagctcaccc
     4381 tctaaccggc acttttgctc cacggcttcg gcgagcgtcg cgagtgatgt gccggattca
     4441 gcgcgcgcca cttgcacgga agcgtgaaac gcctcgagca gaggaaaact ccgcacgtcg
     4501 cccagaaaaa tccggccccc cgggcgcaat tttggagcga ggcccgcgag cacccggcgc
     4561 aggtagtcga ggctcgggaa atattggatc accgagttga gcacgatgac gtcgaatgct
     4621 gcgtcgggca gcgtctcggt agtgtgcgcg ggctgctcca tcagttgcac gtgggcgaag
     4681 cgaggtccgg catcggccag cgtacgccgc acaaaggcga ggagctcggg cgcaaagtcg
     4741 gtgccgacgt agcgcgagca gtgctcggcg atctgcgtca agagtagccc cgtgccgcaa
     4801 cccacctcga gcacggtctc tggatgccat gagagtacgc gctgcgtgat cgcgtcgact
     4861 tgctcttgta tctccgccgg tggcagctct tgctgggtgt aggagctcac ccagccgtga
     4921 gtcgtgaacg tggtggctgc ttgtcgcgct agcgcggcgt gtgtttcggc gaagacttgt
     4981 ctccactcat cgactcgtgc gtcgacgagc tgcccctcaa gcgtcgcgtc caacttgctc
     5041 gggaccacat aaccgatcag ccgccgttgc gtcccttccc cggagacacg caccaacgcg
     5101 tccctcaccc tcggctggcg cctgagggaa gccgcgacct cgttcagctc gacgcggtaa
     5161 ccgcggatct tcaactgctg atcgcgtctg cccagaaatt cgacgctacc gtccggcagc
     5221 acccgggcgc gatctcccgt gcggtagagc cgccccgaac gctgcgagaa tggatcaggc
     5281 acgaaactcg ctgcagtctt gcgcggattt ccgaagtagc cgcggctgac ctgcggccca
     5341 cccaggtaca gttcgcccgc gacgccaatg agcgcgggtc gcaatgcgtc gtccaacacg
     5401 tgcgcactcg cgcgcctcaa cgcgatcccg agtggcaccg tgcttgacca gccgcgcggc
     5461 tccgcttgca cgcgttgcgc gatcacgcca acggtcgtct ccgtcggccc gtagtgattg
     5521 acgaccgtgc acctgggtcg gacttggctc acacggtcaa acagctccca gctcgtgctc
     5581 tcgccgccca gtaccaggaa gcgctcgggc agcgcggctc ctcccggatc tgcgtcgagc
     5641 agagcggaca ggtgcgaggg cgtaattttg agcgcctcga tcccattctc gcgcacgtag
     5701 cggccgaacg ccaccgcgtc caacaccagc tgcttcggca ctaaatggag ggtcgcgccg
     5761 aagcagagcg cgccgaagat ggccgtattt cctagatcgg aggccaccgt cgtgacgctg
     5821 gcccaactgc gcagctcggg gagcggaagc actttctgga gcgccaagag gtaggagagc
     5881 atttggtcgc gctccacgat cacgccgttc ggcactcccg ttgagcccga ggtgtggatc
     5941 acgtacagcg cggtccggcc gtgggtggcc gcgatccgag ccgcgctcgg atcggcaagc
     6001 aggtcagcgt aggttgcgag actaggtcca ttgaagcgcg tgacatgggt cgagtccgcg
     6061 atcaccagcg ccgcgcgcgt gcgctccgtc aacagacaca agcgctcgtc cgggtgctgc
     6121 ggatcgaggg gcacataggc ggctccggcc ttcaccaccc ccaacagaga ggcgatcagc
     6181 tcgactgagc gatccatgca cagcgcgacc cgcgactcgg ggccgatccc gcggcgttgc
     6241 agggcacccg caatgtgcgt tgctcgtgcg tcgagctctc gataggtcaa ggcgtcggag
     6301 ccacactgga ccgcgacgcg gttcgggtac cgaagtgcga cgcgctcgaa ccaggagacc
     6361 agatccgccg gctcgtgtcc gttgtcgggc agcccgcccc gcatcagaat ttcccgttcg
     6421 cgaggcccca ggcagttagc cgcgtgacag ggcaagctcg gttcctcgag cagctgttcc
     6481 aacagcgtga cgatgcgctc gctgaacagc tgcgcctcgt ccgcgagcac cgagcatgcg
     6541 tcgtaaaaca aagtggcgcg caatcctccc ggcgtgcgcg tgcagctgag cttgagtttg
     6601 aaggtctcta gatgattgaa cacctcctcg atctgaaatg catcagcgcc tatgcgaggc
     6661 tgcggaactt ggatgaactc gaattgtgcg gcgcacggcg cccgatctcg atccagcggg
     6721 tcttgcccgc gcaggtccgg ctcgaaatcg cactgccaga gggaggggct ctgctcggag
     6781 cgtgaggcct cgagttgcag cgcgagccga gcccagttgt cgaacagcga gccctcggtc
     6841 cagactgata cagtgactgg cgccaccgca gaaagcgggc ccaagcaatg ctcgagctca
     6901 gcgtaccagc gctcgtcccg cagcacaccg acccggaact gtgcttcgcc cgagacccgc
     6961 gccagcacca gactgaaggc caacagcagc gctgactcga agctgaaagc atgcgtgtca
     7021 gacagagcct cgagccgcga gctcagatcg acatcgatga cgactgggtg ctgcccgatc
     7081 gcgaactctc cccgatgctg ttcgaacgcg agccgcggat cggccggttg cgggctttcg
     7141 gcgttgctgg cgccgtcgtc cagcaactga ttttgccact cggctgctaa cccgaagtcg
     7201 gggccgtccg gcgtagaggg cggggcaacc tggctgagag cacttccaag ctctgcggcg
     7261 agagccagcc acgttttgct atcggcattt agcgcagggc tttcgacgag cagctcggtc
     7321 gtccccgcgc tcccggtaga caaggagtag ctcacttgat ttccggacgt cgagcgggga
     7381 ccgcgagacg agctggaaac ctgccgcacc gcgggatgtc gctcggccgc ttcggaagat
     7441 gagcgaggcc tgcactgaag agcaccacag agtacggctc gaggcagcga aggctctgcc
     7501 tccgtcgcgc ccgcgttcgc gaacgcatag cgcaggaccg gatgtcgctg accgagcccg
     7561 ctcagcgccc gctgcaacgc ttgcgctgat gggcgccgtg ccagcctgag gcgcacgccc
     7621 gccgtcaacc acgcgtcgga cggctgctgt cgccaggttc gccgctgttg cggagagagc
     7681 cgatattcct tgccgctcga cgcctcggtg ggcgtgacat cgaggctcgc ttccgtcact
     7741 gctgcgcgca tgagcgtcca tcccgttctg tttgaggttc agccatcgaa accaagatct
     7801 tgcgcgggcc actgaacggt tcccggccgt gcgcgaccag cacgttgtcc accaacatga
     7861 cgtcccggcg gcgccagtcg aagcgtctgg ctaccgcacg gtagctatct cgaatgtgtt
     7921 cgatataggc gtcgggtatg gggctaccgt ccccgtagta cgcgtgacgc gggagatccg
     7981 aatcagcaaa gagctcacgc agcgaggcgt aggtcgcctg ctggtgcgct cgcacatgga
     8041 acatgtgcgc cgagttgaac cacactggct cgcccgtgtg ctcgtgccgc agtagcgcct
     8101 tgtgaacctc gagggtgcgt agtcgatctc cgccgagcca cgaccactgg atgtcgtgct
     8161 ggcgacaata gctctccacg cgatcgcggt cttgggttcc gaacacctcg tgccatggga
     8221 cgtcgaggcc cgagccgtag ttgcgctgat acatgacccc cttgcgctcg aaggcgtcgc
     8281 gcacctcgcg gtccaacctc gcgagcagcg cgcgcccgct cgcgagcggg gtcgcgccct
     8341 gggctgccgg tggggtgtgg cagtagaacc agatcttgcc gggccaacag tgtgaatagg
     8401 agagctcatt gtgaagcatg atgctctctg aggcggggta ctcggtagat gtgtagatcc
     8461 gctcgcgcac acggcttcgc ggcgtggagg cctgctggta ctcctcgagc cgaggggaca
     8521 tggccagaca aagagcttcg aaatcggcgg catcgctcac atcgaagccg cgaaacagga
     8581 ttgctccctg ttcggacaac cgctgctgca cgagctggcg ctgctctcga atccagtcgg
     8641 cgaggttcac gccgggagcg cgaggcgtga ccagcagaat cagtggatcg tctggccaat
     8701 agttccgagt actgaccagc tgctccgtcg atggcgggga gccctggccg cgcttcagga
     8761 tcgactgtag ccctgctctt cgcgagggaa gcggctctgg aatcgagcgg gtcgggttca
     8821 ggctttgagg taacggcacg tcagagacgc gtgcgtgcaa gtgatccacg gcatagcgca
     8881 ggacatcgac gtagtacgcc aacagatcgc gtccgcacgc ctcggagatc ttgctcgcgt
     8941 cgtgctcgag gtgggcccgc aactgcccgc tggcctcctc gatgcggagc gtcaggtcgc
     9001 ggttcgccgc gccgcggtcg aactcgaccg cctcccagtc gatcgaggtc ggctccagca
     9061 agcgtcgcgt gggctcgttc aaatacgtga acatcacgcg aaacagagcg ctgctcgcct
     9121 ccccgcgtgg caaattcagc gtctcgtaca cgctgtcgaa cggcacgtcg cgatgattga
     9181 aagcgttcaa gacgacgctc cgagcttgct tgagcacgtc gtgaatgctg aagctgcgcc
     9241 caatcctgag ccgcagcact agcgtgttga cgaagaaccc gatcagatta tgcgtctcgg
     9301 gtcgtgtacg ctcggcgatc gggctcgcaa tcagcaagtc gtcactgccg gtccagcgat
     9361 gcagcaacac gccccatacc gcgatcagga gcatataggg agtgataccc gcctcgcccg
     9421 ccacgcgtgt caccgcgttc gcgaggtctt cggggatggc tgcaaagtga gtgacaccgg
     9481 tgttgacgcg cccgggagcc gtgggcaatg ggaagcgaat gggcggcggc agatcggcca
     9541 ggtttcgttg ccaatactcg agctgcgccc gccgttcttc cggtgccagc gcgtcggcct
     9601 gccaagccgc gtagtcgccg tactggattg cgagctccgg tagctccggc aagctgcccg
     9661 ccaaggcggc ttcataggtg cgcgtcagtt cgttcaccag caaccctacg gaccagccgt
     9721 cgcatacgat gtgatgggcg ctcacgatca gggcgtgatt ctccgccccg aactcgagca
     9781 ggcgcgcgcg cagcaggggg ccggtttcca gccgaaacgg ccgctggctc tcttcttgcg
     9841 ccgcccgcac gatagcctgg atgagggttg actcgggcag ctccgcgcag gcgcttcggc
     9901 tcaccttgag cgccgcgttg ggcaacacac attgggtggg aacgccgcct tccatcgtga
     9961 tcacgctccg gagcacctcg tggcgctgca cgatcgcgcg taaggcctgg atcgcgatct
    10021 cggctcggaa cgagccgcgg atacgcacgg ccgcggtcac gttgtactca gtgccttccg
    10081 gcttgagctg gtacaaaaac cagagcctgc gttgctcgct cgaaagcggg aagcgggcgc
    10141 cagcgcgcct cggaatcccc gctgaaggcg cgacgctcgg gcgcgagtga ctcacgcgct
    10201 cggccaagct cgcgagctct ggtgcttcga acagcgccga caacggcagg cgaacgccga
    10261 atgtctcctc gatgcgcgaa acgagcagcg tgcccaagag cgagtgcccg ccgaggtcga
    10321 agaagctgtc ctccgcgcca atctgttcgg gggatatctt caggagctcg gcccaaatcc
    10381 gggccagctg gtgttcggct tcgccgctga gagcgcgttc gttcgccgcc tcggagtggg
    10441 cggagtgccg cgactgctcg gcgagcgcgg cgtggtccac cttgcccgcg gcgttgaggg
    10501 gcatgcggtc cacgattcgg atctccgcgg ggaccatgta cgaaggcagc cgctcccgca
    10561 gttgagccgc tactcgctgc ggaaccaacg tcgacgaagg ggtccgctcc acaattgcca
    10621 cgagcgtgca gctaccggcc gcatctcgac caccgacgcg taccgccgct tgcctcactc
    10681 cctgcaagcg aacgagctgc gcttcgatct ccgcgagctc tacccgctgt cccctcacct
    10741 tcacctgacg atcttggcgg ccgcggaact ccaacaagcc ctcgtcgttg atccgccccc
    10801 tatccccggt tcgataaagc cgcccgggcg gaccctgcgg ggcggggacg aagcgcttcg
    10861 cgctctcgcc gggatctctc caatatccgc gtgcgaggcc gctacctgcg atgtaaatct
    10921 ccccgcacac gccccgtgga accgggtcgc cggctgcgtc caagatgctg atggtcgttc
    10981 cgggtaacgg cttgcctaga ggaatgctgc cccgaacttc gtctcgcgca ctgagcacgt
    11041 gcactgcgct caccacacaa cactcggtcg gcccgtagct gttcaggatc tgcacgtggg
    11101 aggggagccg ccgccgccac ccgaccaacg cctcgtggtc cgcggcttca ccgccgatca
    11161 cgatcgacct cagccgcgag agatccggga gcgaaccgat ccgtgagagc tcccgaccga
    11221 gtaagttcca gagcgcggtg ggcagatcga gcacactgat ccgttcgcgc gccacgaact
    11281 cgaggaagct gagcgatccg gtgagcatcg actcgtctcg caacaccaac gtggcgccgg
    11341 cggtgagcgt ggcgaagatc tcttcgaggc tcgcatcgaa agccaagttg gcgaactgca
    11401 gtacacggtc ggtgggcccg agctgcatgt actggcgagc cgcgagcgcg aagtggtgca
    11461 gcgcgccgtg tgacatcgca acggctttgg gtcgcccgct gctgcctgag gtgaacagaa
    11521 cataggccaa ctcaccggcg ctgtatgcgg ctttagggct ccacgcgcgc gaggatgaag
    11581 tgaggggctt cttcggcgcc tccggctcgg gcgtgatcac gtgtaacccc aagtcactca
    11641 gcagcggtgc ccattcgcgc tctgccaccg cagcgcgagc ccccatgcgt tgcacgagct
    11701 cgcgcacacg ggccacgggg gttgccggat cgatgggaac atacacaccg cccgcccgaa
    11761 gaatggccaa gatcgcagtg atcgcctgca ggcggcgcgg caagaggtga atgaccggct
    11821 gctctggagc atcgagagcc tcggccagct gcgaactcaa ggcatcgacg gacgcccaaa
    11881 gctcggcgta agtcagcgct tgaccggcgt gttcgatcgc gatccggtcc agcgtgcggc
    11941 tgtcgaattc gatttgatcg agcagtccgc ccggagcacc aaccaaggcc ggcgccccgc
    12001 ccaagctggc ttcgctccaa tccccccgag agaagagcga cagcggtgaa tgcggggccg
    12061 tctcgtattg ctccagtgcg cgctcgaaat ccgcacagaa ccgtgccagc tcagcctcac
    12121 ccatgacgtg cttttgagcg ttgaacttga gcgtcaattc gccgttcgac cactccgcgt
    12181 gaagcgcgac ccggtcgtac tcgtgcgacc cgttcgacag ccgcagcgtc gcggagcagc
    12241 cttcgaatga tggcgactct aggcttacga ggttgaagag cacgtcgtag acgcgctcct
    12301 cggcgggatt atccacgaca tacgtggcgt ggcgcatcat ctccgcggac gcctggagca
    12361 gctggcgccc caaggcgacg aagtctcgtc cggcatcgac ttggctgcgc agcacgccta
    12421 ccgacatgag cggccccacg attcgtcgtt cctccgggcc agcccggttg gccaacgtga
    12481 cgcccaagct gtgctcctcg acaccggtgg cccgtcgcag agtcgcgagc agcccgatca
    12541 aaagcacgtt agcgagggtt tggagtcgtg agaaaccagc cactccgttg ctccgaccgg
    12601 aatcgatcag gcgccccaat cgcgcagagg acagcggcag gatccggctg tgaatctcgg
    12661 tcgtttccgg tccgcgcttg tgaccataga attcgagcgg agtcggcttc gtgctgagtt
    12721 tctgcttcca gaaggtcgca ctggagcagt atcccccctc ctcgtgcttg cgcgcgtcgc
    12781 gctccagata ggcttcgaac cccggatagt tctcggctcg gtcggtgccc gcgtagatct
    12841 cggacaagcg tcgaaatagc tcaccgagcg cgtgcccgtc gatgaggagg tgatgaatcg
    12901 tcagcaccag atgatgtcgc ccccgagcgg tctctaggag cacggcgtcc cagtgcggtt
    12961 cccggttgca gagcgacagc gttgcgcgct ccttcgaaat ttcctcgagc cgagctggat
    13021 cggcgggcac gagcggcagg gtcccgaagc gcgctccccc gaacacctgc cgcaatccat
    13081 catcccgagt gatgaatcga gtctgcagga tatccgtcac cgccaccaaa cgcgagaatg
    13141 cgttctgtag cctcccgaca tcgagctccc cctcgagctc gagcacggct tgttgattgt
    13201 aaagcggcag ctcgggatag cgggcttgac cgagccacat cagcatttga gcgcgagacg
    13261 gtttgcattc ccgttcccgc agcaccgctg gcggctctcc caacggcgcg tcggcgtgag
    13321 tcaccagcct ttcgaggcag gccacgaacc ggatcgctag atcgcgcacg ctggcaagct
    13381 cgaggcgcga gcgggagaac tctatgacca actccgcgcc atctgcggtg tctctcaggg
    13441 tcgcgttgag ctcgatcgcg acgtcgggcg cgaatacgtc aacgaccgag aagcgtgccc
    13501 cctcgagctc gaatcggcgc gcgaaattct gaacactgaa ggccacgcaa gccatcgggt
    13561 tcagagtagt tccgcgctca ccctgcagcc gtgaggccag gatgtgaaac ggcacgtctt
    13621 cgaactcctg cgcttgcagg accgcgcgtt gcactcgagt cagggcgtcg gtcgaggtga
    13681 tgaccccgga aaggtcagcc ctcaccaggc ccgtgttcgc aaagcaaccg atgagcggcg
    13741 ccacgtcggg taacccgcgg tttgcgagcg gtgtgagcag cccgatgtcg tcgattcccg
    13801 attcggcgcg gagggcgaga gagaaagccg tcagcgacac cacgaatcga gtcgcacttc
    13861 gctcacgcgc gaagtgatcg agtcgtcgac gaaggtccgc gctgagcggc tggatatgca
    13921 cgaccttcgt ggagctaccg cacggcgcgt tcgaggagcc ggtcccttgc gatcgtaggc
    13981 gcaaagcgtg gggcggcgct gttccaccct gccgcacgtg ccaggcctcc gcccgtcgag
    14041 caagctcggt atccgatgcc tgagcgcgtt gccaaaagca gtagtccgcg tattggatcg
    14101 cgagctctgc gggttcgctt cgcgtccccg agcggcgtgc ggcgtactgc gcggcaaact
    14161 cttgcaacag cacatcgatc gaccagccat cgaaaaccag gtgatggaag acgagcagca
    14221 gaactccact ccgatcgcca gtttccaaca gcaaagaccg aaacaggggg gcttcggcca
    14281 ggtcgaaggg tcgtgccgcg aactccgcga gcaacgcagc caccctccct ggatcggcat
    14341 ttcgcacgtg ctcgaatcga agcggcgcgt ccgcaacaac ggtctgaaaa ggctctccgc
    14401 gacgctcgcg caccgcgcta ctgagcacct ggtgtcgacc ttgaagggcc tgcaacgtaa
    14461 ggcggagcgc cgctacgtcg agctccccgt cgaactcgag acagatcgga acatggtagg
    14521 cgctgccggt ggagccaagc tggtgcagaa accagagcct ctcctgagcg aaggagaggg
    14581 gcgcgtcccc tggtcctcgt cgcaccaaac gcggccctcc gccggcgagc cgcgcgcgca
    14641 gccggcgttc cagcagctcg cgccgcgccg ggtcgagtgc caatacgcgc tccgcgctaa
    14701 gtggtttcat ctgccccgtt cctttccgcc gctga
//
LOCUS       DASTKU010000546         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_106097, whole genome shotgun sequence.
ACCESSION   DASTKU010000546 DASTKU010000000
VERSION     DASTKU010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106097"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..804
                     /gene="rdgB"
                     /locus_tag="VFQ61_18950"
     CDS             163..804
                     /gene="rdgB"
                     /locus_tag="VFQ61_18950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HET9956592.1"
                     /translation="MTVHDVLLATRSAGKLLELRPLFDAHGVRVIDLDDAGIPESAAE
                     DQLEAYETFEENALAKARHFHRLSGRPTIADDSGLVVDALGGQPGVHSKRWSGRDDLR
                     GIALDEENNRRLLERLRGVADRRAHYVCVAAYVDGDRELTFRGEVHGRIVDDRGGPSG
                     FGYDPYFHCDELGRTFGDATREDKELVSHRGRAFRGLIAALEGVGRMDREGGR"
     gene            867..940
                     /locus_tag="VFQ61_18955"
     tRNA            867..940
                     /locus_tag="VFQ61_18955"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:901..903,aa:Pro,seq:tgg)
     gene            974..1047
                     /locus_tag="VFQ61_18960"
     tRNA            974..1047
                     /locus_tag="VFQ61_18960"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1008..1010,aa:Arg,seq:tct)
     gene            1086..1158
                     /locus_tag="VFQ61_18965"
     tRNA            1086..1158
                     /locus_tag="VFQ61_18965"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1119..1121,aa:Lys,seq:ctt)
     gene            1701..>2151
                     /locus_tag="VFQ61_18970"
     rRNA            1701..>2151
                     /locus_tag="VFQ61_18970"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          399 a          571 c          782 g          399 t
ORIGIN      
        1 tcgtgatgag cagcgaaggc cggttcgtgg aagtccaggg caccggcgaa cacggtacgt
       61 tcgatcggct cgagctcgat gcgctcctgc agttggcgat caaagggttg cgcgagctcg
      121 atctggccca gcagcacgtg ctcggccgct gacgcgcgcg ctgtgacggt gcacgatgtt
      181 ctgctggcca cgcgaagcgc cggaaagctc ctcgagctgc ggccgctctt cgacgcgcac
      241 ggcgtccgcg tgatcgatct cgacgacgcg gggattcccg aatcggccgc tgaagaccag
      301 ctggaggcgt acgagacgtt cgaggagaac gccctggcaa aggcacggca ctttcaccgg
      361 ctcagtgggc ggccgacgat tgcggatgat tccggcctcg tcgtggacgc gttgggcggt
      421 cagccgggcg tgcacagtaa acggtggagt ggtcgtgacg acctccgggg catcgcgctc
      481 gatgaggaga acaatcgccg gctgctcgag cgcctgcgcg gggtggccga ccggcgtgcc
      541 cactacgtgt gtgtggcggc gtacgtcgac ggcgaccgcg agttgacgtt ccgcggcgag
      601 gtgcacggca ggatcgtcga tgatcgcggc gggccgagcg gcttcggcta cgatccgtat
      661 ttccattgcg acgagctcgg gcgcacgttt ggcgatgcga cgcgtgaaga caaggagctg
      721 gtcagtcatc gcggtcgcgc gtttcgagga ttgatcgcgg cgctcgaggg agtcggccgc
      781 atggatcgcg agggcgggcg gtgacgcggg aacgcactcg tggctagttg attccgctgg
      841 atttggcgga tacattcgaa ggctcgcggg gcgtagcgta gcctggtatc gcgcctgctt
      901 tgggagcagg aggtccccgg ttcaaatccg ggcgccccga cctcacggca cgaccggcag
      961 cattgtgcgt gaagcgccag tagctcagtt ggatagagca acagccttct aagctgtggg
     1021 tcgggggttc gattccctcc tggcgcgtct ggtacagcga cggtgcatac gcgtgagacc
     1081 gcctcgggcc gttagctcaa ttggcagagc aagcgactct taatcgctag gttgtaggtt
     1141 cgattcctac acggcccatg agcaccagca gttgggctcg gtggagtgtg acgagcgagc
     1201 tccggcggaa ttgtcgggct cgaggtgaag cggcgggaac gtgtggtgga ttttggggtt
     1261 gaaacccgcg cttcagatgc ttactatcag gacccgccgc gatgtggcgc gacgcaggga
     1321 tgacgcgtac ggatcggggc gagcggggct cgaggcgttc gatcaagcgg gcggaggtgg
     1381 cccgatcgag tgcagagcgc tgggcggagt tggtgatttg catggtggat tttccgcgag
     1441 agcgcttgac ggtgtgcagt gattgcggta tgattaaagg ctgtcgctca cgcgacgcga
     1501 aatgagcata acgctgtttg ataattgagt ttgggaatac ggatgtgcga ggcggactcg
     1561 gcgatccgat gctcgttcag ggcgtcgggg agagttcaga cctgaacatt tctgtgattc
     1621 ggacacgctg ttgatcatgc aattgcgatc aagcgacagc gtgagcaaca gcggagtctg
     1681 atcgagctat gtcaaaaact ccattggaga gtttgatcct ggctcaggac gaacgctggc
     1741 ggcgtgctta acacatgcaa gtcacggggg ctcgtagcaa tacgggcaac cggcgaacgg
     1801 gtgcgtaaca cgtgagcaac ctgccgcgct ctgggggata gccggcccaa cggccgggta
     1861 ataccgcata cgttcttcga ggggagtccc ttgaagaaga aacctccggg ggagcgcgag
     1921 gggctcgcgg cctatcagct agttggcggg gtaatggccc accaaggcag tgacgggtag
     1981 ctggtctgag aggatggcca gccacattgg gactgagaga cggcccagac tcctacggga
     2041 ggcagcagtg gggaatcttg cgcaatggtc gaaagactga cgcagcgacg ccgcgtgtgg
     2101 gaggacgccc ttcggggtgt aaaccactgt tgcccgggac gaaactcctc t
//
LOCUS       DASTKU010000547         6091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_328624, whole genome shotgun sequence.
ACCESSION   DASTKU010000547 DASTKU010000000
VERSION     DASTKU010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328624"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1502)
                     /gene="groL"
                     /locus_tag="VFQ61_18975"
     CDS             complement(<1..1502)
                     /gene="groL"
                     /locus_tag="VFQ61_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET9956593.1"
                     /translation="MAAKEIVYQERARNLILNGVNALADAVKVTLGPRGRNVVIEKSF
                     GSPTVTKDGVTVAKEIELDNKFENMGAQMVREVASKTSDIAGDGTTTATVLAQAIFRE
                     GSKLVAAGHNPMEIKRGIDKAVESITGELKKLAKSTKDPNEIAQVGTVSANGDETIGK
                     LLSEAMEKVGKEGVITVEEAKSAETTLEVVEGMQFDRGYLSPYFVTDPERMESSLEDC
                     YLLLSEKKISNMKDLLPVLEAIARQQKPLLIIAEDVEGEALATLVVNKLRGTLSCAAV
                     KAPGFGDRRKEMLKDIAILTGGQVIAEELGLKLENVTLSDLGQAKRVKIDKDNTTIVD
                     GAGKKDKIKGRQQEIRGQIEATTSDYDREKLQERLAKLVGGVAVIKVGAATETEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALIRAQKVLEGLKLNDEQQFGVKIIYRSIEEPL
                     RQIVANAGEEGSIVVQKIKEGSGNFGFNAATTKYGDLVADGVIDPVKVVRS"
     gene            complement(1523..1813)
                     /gene="groES"
                     /locus_tag="VFQ61_18980"
     CDS             complement(1523..1813)
                     /gene="groES"
                     /locus_tag="VFQ61_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET9956594.1"
                     /translation="MKIRPLQDRVIVKRVKEEEKTKGGIIIPDTAKEKPVEGEVLAVG
                     NGKVLEDGSVRKLDIKAGDRVLFGKYAGTEVKIEGEDHLILREDDILGVIEK"
     gene            2319..2963
                     /locus_tag="VFQ61_18985"
     CDS             2319..2963
                     /locus_tag="VFQ61_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956595.1"
                     /translation="MHVTNRDPQLALAADLHGFFQEALVSALQERGVEVTEPTSHYLV
                     ALLADFAKPGELTQEALERPLAVLLQEALGLAGRERFERLRSLGDSVLYTSGFFFDHL
                     ETRGVTLTYVSSLGARAYGGAASMLRYAAPEKPAHGEAPRAPELFEELADNFAHFAQV
                     LATVADGLYANASRSTHAGMLKVYERWLRTGSNQLAQALTERGILPTRGSGGVH"
     gene            3074..3580
                     /locus_tag="VFQ61_18990"
     CDS             3074..3580
                     /locus_tag="VFQ61_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956596.1"
                     /translation="MLEFVREVEDTERETLLVREHEDSVELALLLPGRLLEERADHDD
                     DGLLQAFEGVSHFVYLAERVRMELPTTRLELELQAEVDKFVLLTHGRDRLEPLERDRL
                     CEHLFERVSYLHHETTEEGARYRLANALAARFVRRLDVRAPGALRERLRSFYRAGQAD
                     KIRLASAA"
     gene            complement(3620..5008)
                     /locus_tag="VFQ61_18995"
     CDS             complement(3620..5008)
                     /locus_tag="VFQ61_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HET9956597.1"
                     /translation="MLNPSREDARPVKNIDDLLEVFHAAEKPEARFLLGTEAEKFGVH
                     RESYAPLAYEGEHGVARILNALTALGWQPEAETEGGPVIALRREEASITLEPGAQLEL
                     SGAALPDVHGTALEISGHLRELAQISSEMKLVWLSVGFHPLARQLELPWVPKQRYAIM
                     REYLPPLGHAALDMMRRTATVQVNLDFSSEEDAIRKLTVGLKLAPLIHAMLANSPFRE
                     LRAGGPKSLRGQVWLNMDRSRSGLVERVLNAPRPSYKDYIEWALDAGMFLLKRGERVL
                     ANTGQTFRDFLLNGYSGEHATVADFKLHLNTLFPEMRLKNTLELRSVDALPARLMTVV
                     PALGAGLFYESRALAEAEALARSIGVSELAFARKDLVEQGLGARVGTRWARDLASKIL
                     EIAHGGLSRRKRLDAQGQDETIYLREVTELASRGLCPADLLRMGLEGSGPYPAATLIE
                     RTRIDAAAPQVL"
     gene            complement(5121..6059)
                     /locus_tag="VFQ61_19000"
     CDS             complement(5121..6059)
                     /locus_tag="VFQ61_19000"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET9956598.1"
                     /translation="MSFEHIRDRLQQGRPLILDADMGASLRARGVKLDVPGTLGQMLR
                     EQPHEVFQHHLREVECRVDVISALTADTTPRALSEVGMQHRAAQLTSRAVDLALDATQ
                     LAHKPVAVASVLGSDMVAPTIAERLAEELTEHAARLRVAGSELIIARGLGSRRSLMAA
                     LTASRQTELPTWAVWECDGSEEDASPEALKELLSELSAACASVVLFEVRSVETGVETL
                     RRARVACERLNLVPGILLAASNDSVRGFDDPTSEPEHWAERALELCDAGARVLGGGAG
                     TTESHTRALAFALGSLHPSGAPCATEVTLCGDRGGC"
BASE COUNT         1041 a         1937 c         1918 g         1195 t
ORIGIN      
        1 gagcgcacga ccttgaccgg gtcgatgacg ccgtcggcaa cgaggtcgcc gtacttggtg
       61 gtggcggcgt tgaagccgaa gttgccgctg ccttccttga tcttctgaac gacgatcgag
      121 ccttcttcgc ccgcgttcgc cacgatttgg cgcaagggct cctcgatcga gcggtagatg
      181 atcttcacgc cgaactgctg ctcgtcgttg agcttcaagc cctcgagcac cttctgggcg
      241 cggatcagag cgacgccgcc gccgggaacg atgccttctt ccaccgctgc gcgggtggcg
      301 tggagggcgt cctcgacgcg agccttcttc tccttcatct cggtctcggt ggctgcgccg
      361 accttgatca cggccacgcc gccgaccaac ttcgcgaggc gctcctggag cttctcacgg
      421 tcgtagtcgc tggtggtggc ttcgatctga ccgcggatct cctgctggcg acccttgatc
      481 ttgtccttct tgccggcgcc gtcgacgatc gtggtgttgt ccttgtcgat cttgacgcgc
      541 ttggcttgcc cgaggtcgct gagggtgacg ttctcgagct tgaggccgag ctcctccgcg
      601 atcacctgac cgccggtcag gatcgcgatg tccttgagca tttccttgcg gcgatcgccg
      661 aagccggggg ccttgacggc ggcgcaggac agcgtgccac gcagcttgtt caccacgagc
      721 gtcgcgaggg cttcgccttc gacgtcttcc gcgatgatga gcagcggctt ctgctgacgt
      781 gcgatggctt cgagcacggg cagaagatct ttcatgttgc tgatcttctt ctcgctgagc
      841 agcaggtagc aatcctcgag ggaggattcc atgcgctcgg gatccgtgac gaagtacggc
      901 gagaggtagc cgcgatcgaa ctgcatgcct tcgacgacct cgagcgtggt ctccgcgctc
      961 ttggcctctt cgacggtgat gacgccttcc ttgccgacct tctccatcgc ctcgctgagc
     1021 agcttgccga tggtctcgtc gccgttggcg ctgaccgtac caacctgcgc gatctcgttc
     1081 ggatccttgg tcgacttcgc gagcttcttc agctcaccgg tgatggattc cacggccttg
     1141 tcgatgccgc gcttgatctc catcgggttg tgccctgcgg ccaccagctt gcttccttcg
     1201 cggaagatgg cttgggcgag cacggttgcg gtcgtggtgc cgtcaccagc gatgtcgctg
     1261 gtcttcgacg cgacctcgcg caccatctgg gcgcccatgt tctcgaactt gttgtcgagc
     1321 tcgatctcct tggccacggt gacgccgtcc ttggtcacgg tgggagaacc gaagctcttc
     1381 tcgatcacga cgttacgacc acgcggcccg agggtgactt tcaccgcgtc ggccagcgca
     1441 ttcacgccgt tgaggatcag attacgtgca cgctcttgat aaacaatttc tttggcagcc
     1501 atggctcacc gtccttctaa attcacttct cgatgacgcc gaggatgtcg tcttcgcgca
     1561 ggatgagatg atcctcacct tcgatcttca cctcggtccc agcgtacttg ccgaacagca
     1621 cgcgatcgcc cgccttgatg tcgagcttgc gcacgctgcc gtcttccaag accttgccat
     1681 tgccgacggc gagcacttcg ccttcgaccg gcttctcttt ggcggtatcg gggatgatga
     1741 tcccgccctt ggtcttctct tcctctttga cccgcttcac gatcacgcgg tcctgcaacg
     1801 gtcggatctt catcacgtct ctcctgttcg tggttggcgg aggccccgaa ggctccgcgg
     1861 caggctccgt cagagctcca ggccgtgacg gcgcacaaaa gcaacccctc ggaaccaatg
     1921 aggtttctag gttgtcagca ctctcgcgag aggagtgcta gcgagcgggg aacttacgca
     1981 cggtctttcg ggtgtcaagg gaggcacccg aacggcgccg atttttcgac gcggtttcct
     2041 cgccgctgcc tacacgttca gcctgcaaga ttggcagccg gtgcgcgaga ttgctgagga
     2101 aatggacttc ggctgtgcag acgtgcagcc ccaccagcgc gtcgccgggt aaaactaaac
     2161 gagttcgtga cgatcttggc gtcccgattt cccgcaccga agtggtgctc ggccttacag
     2221 ccgcagcccg acactggggt accgccggag aggggagcaa tcggcctccc gacggatacg
     2281 gggcgtcttt ggaattggcc ggaggattgc agaaagccat gcacgtgacg aatcgagatc
     2341 cgcagcttgc attagccgct gacttgcacg gtttcttcca ggaggcgctc gtttctgctc
     2401 ttcaggaacg cggcgtcgaa gtgacggagc ccacctctca ctacttggtg gctctcctcg
     2461 cggactttgc gaaaccaggg gagctcacgc aggaggccct cgagcgcccg ctcgcagtcc
     2521 ttctgcagga ggcgctcggt ttggccggcc gcgaacgctt cgaacgactg cgaagcttgg
     2581 gagactcggt cttgtatacg agcgggttct tcttcgatca cctggagacc cgtggcgtga
     2641 ctctgactta cgtgagttct ctcggcgcgc gagcctatgg gggagcggcg agcatgttgc
     2701 gttacgcggc gccggagaaa cccgctcacg gagaggcgcc gcgcgcgccg gagctgttcg
     2761 aggagctggc cgataatttc gcccacttcg cccaagtgct cgcgacagtg gcggacggcc
     2821 tgtacgcgaa cgcctcgcgc agcacccacg ccgggatgct caaagtgtac gagcgttggc
     2881 tgagaaccgg ttctaaccag ctggcgcaag cgctcaccga acgaggcatt ttgccgacgc
     2941 gcggcagcgg gggcgtccac tgaaaggtcg gggtgcgggg ggaggttcgc gcagcagcaa
     3001 gcggcggggc aggcttctgc gcgggataca gcggaaactc gagcggttct acggactaga
     3061 gctgggaacc gacgtgttgg agttcgtccg ggaagtagag gacacagagc gtgagacctt
     3121 gctggtgcga gagcacgaag acagcgtgga actcgcgctg ctgctacccg gtcggttgct
     3181 cgaagagcgg gcggatcacg acgacgacgg tttactccag gcgttcgaag gcgtgagcca
     3241 tttcgtatat ctggccgagc gagtgcggat ggagctcccg accacgcggc tcgaactcga
     3301 gcttcaagcg gaggtggaca agttcgtact gctgactcac gggcgggatc ggctggagcc
     3361 gttggaacgc gacaggctgt gtgagcacct gttcgagcga gtcagttact tgcaccacga
     3421 gaccaccgaa gaaggagcgc ggtatcgatt ggcgaacgcg ctggcggcgc gcttcgtgag
     3481 acgcctcgac gtccgcgctc ccggcgcact gcgggagcga ttgcgctcct tttatcgagc
     3541 aggtcaggct gacaagattc ggctggcaag cgcggcctag ttcgcgagaa gctccctcgc
     3601 ttgggctccg gtgggcaagc tagagcacct gcggcgcggc cgcgtcgatc cgcgtgcgct
     3661 cgatcaacgt cgcggccggg tacggcccgc tgccctcgag acccatacga agcagatcgg
     3721 cagggcatag tcctcggctc gccagctcgg tgacctcacg caagtagatc gtttcgtcct
     3781 ggccctgtgc gtcgaggcgt tttcgtcgcg acaaaccgcc gtgcgcgatc tcgagaattt
     3841 tcgacgccag atcacgcgcc cagcgcgtcc caacccgcgc tcccaagcct tgctcgacca
     3901 gatcttttcg tgcaaacgca agctccgaca cgccgatgga gcgcgccaga gcttcggctt
     3961 ctgccagcgc ccgggactcg tagaaaagcc ccgcgccgag ggcagggact accgtcatca
     4021 atcgggctgg cagcgcatcg acactgcgca gttccaacgt gttcttgagc cgcatctctg
     4081 ggaacaaggt gttcaggtgc agcttgaaat cggccaccgt cgcgtgttcg ccggaatagc
     4141 cgttgagcag gaagtcgcga aaggtttgcc ccgtattggc gagcacccgc tcgcctcgct
     4201 tgaggaggaa catccccgcg tcgagcgccc actcgatgta atccttgtaa ctcggcctcg
     4261 gcgcattcaa cacgcgttcg accaggccgg aacgcgagcg atccatgttc aaccaaacct
     4321 gtccgcgcag gctcttgggg ccacccgcac gcagctctct gaacggagag ttcgcgagca
     4381 tggcgtgaat cagtggcgcc agcttgagcc cgaccgtcaa tttgcggatc gcatcctctt
     4441 cgctggagaa atccagattt acctggaccg tcgccgtacg gcgcatcatg tccagcgcgg
     4501 catggccgag cggcggcaag tactcgcgca tgatcgcgta gcgctgcttg ggtacccaag
     4561 gcagctcgag ttgtcgggcg agtggatgaa acccgacact gagccaaacc aacttcatct
     4621 ccgacgagat ctgcgcgagc tcacgcaggt gtcccgaaat ctccagagcg gtgccgtgca
     4681 cgtccggtag cgcggcaccg ctcagttcca gctgcgcgcc gggctccagc gtgatcgacg
     4741 cttcctcacg acgtaacgcg atcaccggtc cgccttccgt ctcggcctcg ggctgccaac
     4801 ccagggccgt cagcgcgttc aggatgcgag cgacaccgtg ctccccctcg taagccagcg
     4861 gggcgtagct ctcccgatgc acgccgaatt tctcggcctc ggtgcccagc aggaagcgag
     4921 cttcgggttt ctcggcggcg tggaacacct cgagtagatc gtcgatgttc ttaacggggc
     4981 gcgcgtcttc gcgcgagggg ttcagcatcg catggccggg ggcttgggtt gagtgctcta
     5041 gcaggacgta tgcctagcgg acgtgttctc gttctggacg gaacgtgtgc ccgcgagtga
     5101 taacgtcaag caggcgtggg ctagcagccg ccgcggtccc cgcacagggt cacctcggta
     5161 gcgcagggtg ctcccgaagg atggagcgaa ccgagcgcaa acgccagcgc ccgagtgtgc
     5221 gattcagtcg tgccggcgcc accgccaagc acgcgcgccc cagcgtcgca caactcgagg
     5281 gcacgttctg cccagtgttc cggctccgaa gtcggatcgt cgaaaccacg cactgaatca
     5341 ttcgatgcag cgagcaaaat cccgggcacc agattcaaac gctcacaagc gacgcgggcg
     5401 cgtcgcaggg tctcgactcc agtttctacg ctacgcacct cgaacagcac gacgctcgcg
     5461 catgccgcgc tcagttcgct cagcagttcc ttcagagctt cgggcgaagc atcttcctcc
     5521 gaaccatcgc attcccaaac tgcccaagta ggtagttccg tctgtcgaga tgccgtgagc
     5581 gcggccatca agctgcgtcg tgatcccaac ccgcgggcga tgatcagctc cgagccggcg
     5641 acgcgcagcc gcgcggcgtg ttccgtcaac tcctcggcga gccgttccgc gatggtgggc
     5701 gccaccatgt ccgaaccgag cacgctggcg acggcgacgg gcttgtgcgc gagctgagtt
     5761 gcatcgagcg cgagatcgac ggcgcgggag gtcagctgtg cggcgcgatg ctgcataccg
     5821 acttcggaca gagcgcgcgg cgtagtgtct gcggtcagcg ccgatatcac gtccacccga
     5881 cactccactt ctcgcaagtg gtgttgaaag acttcgtgcg gctgctcgcg cagcatttgc
     5941 cccagtgtgc ccggcacatc tagtttgacg cctcgggctc gcagcgaagc gcccatatcg
     6001 gcatcgagga tcaacggtcg cccctgctgt aaccggtcgc ggatgtgttc gaaggacatg
     6061 actcgaatcg ccgatattca gagcggcagg c
//
LOCUS       DASTKU010000548        12186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1221850, whole genome shotgun sequence.
ACCESSION   DASTKU010000548 DASTKU010000000
VERSION     DASTKU010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12186
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221850"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFQ61_19005"
     CDS             <1..498
                     /locus_tag="VFQ61_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956599.1"
                     /translation="LLQVCLHASMHSFVRSPGLRLYVDIDRAVTDRNPQWESFVQEAV
                     RTGAPGRVGVALGVAQGLLGTPVPLDVIEALIPRGWRRAGLERLLGRESVLGAGRKLP
                     GARTVLLDVLLDPRAMTSWARDVVLPPEDWLREHFGRGSVQKEGVVRLHARRYLNLAL
                     RWRPE"
     gene            508..1152
                     /locus_tag="VFQ61_19010"
     CDS             508..1152
                     /locus_tag="VFQ61_19010"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9956600.1"
                     /translation="MPLARKTELKIKRCIDVVGAATGLVVLSPVLAGVALAIRVTLGA
                     PVLFRQQRPGLGGRPFQMIKFRTMAQAKPGETWYRTDEQRLTRLGRFLRRTSLDELPE
                     LFLVLKGDMSLVGPRPLLMEYLAKYTPEEMRRHEMVPGITGYAQVNGRQNTPFSQRLR
                     DDVWYVDHFSLKLDGEILFKTIGLVFQREGVIPGQNVDDVDDLGLTADRARVSA"
     gene            1149..1685
                     /locus_tag="VFQ61_19015"
     CDS             1149..1685
                     /locus_tag="VFQ61_19015"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9956601.1"
                     /translation="MIHELGDVRLRLPEPSDIDALYRIKNDPELAALLGGHGRGYSRA
                     DLADWISSHRSRRDEVLWVVVDSASEQCIGHVGLYCIDHRVRKAEFGILLERGSWGRG
                     LGRRLTSFAIEYGFRELALSRIELNLLASNARALKTYLACGFVEEGRLRRAQFKNGRF
                     EDVVWMGLLADEYRATSS"
     gene            1745..2875
                     /locus_tag="VFQ61_19020"
     CDS             1745..2875
                     /locus_tag="VFQ61_19020"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9956602.1"
                     /translation="MARIYLSPPDMGPHERSLLLEAFDSGWIAPLGPHVNAFEAEFAQ
                     RVGASHAVALSSGTAALHLALILLGVRAGDEVITSTLTFAATANAIRYVGADPVFIDA
                     EARSWNLDPELLEAELEAGARHGRLPKAVLAVDLYGQLADFERIVPLCQRYGVPLIED
                     AAEALGAHSKGRSAGTFGKFGAFSFNGNKIITTSGGGMLVTEDGAVAERARYLATQAR
                     DPAPHYQHSVVGYNYRLSNLLAAVGRGQLRELDRKVAARTHVRRRYEAELGSLPGLSF
                     MPSADFNQPNHWLTCVTIDPARFGSDREDVRRALEGQDIEARPVWKPMHLQPVFAGFR
                     ARERGVAARLFELGLCLPSGSNLAESDQARVIEIVRAQCRSS"
     gene            complement(2903..4132)
                     /locus_tag="VFQ61_19025"
     CDS             complement(2903..4132)
                     /locus_tag="VFQ61_19025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9956603.1"
                     /translation="MRIVLVNRLLDRPGGEQLQLVQLARRLSGRGHQVHVITGYYDAA
                     ACYPRHLEGLDVRAVHGSRRADGSRSASIFGRTLQHVAFRKRERDLGQSMARLFPADI
                     DLVNWHGALPQPTWNAMHSSGSEVPWVWMCNDLPAPFYQLAALRRGEDKTQLAVQVAK
                     SLPRQLLTQVSVRNPPGIITVLDEDNRWWWRRVLGRDAVRVPWGLEHSTDPVTPTERK
                     PEEPFRLFAAGIIGPLRRFHETIEAVALARSRGLNVTFDICGEPSDPAYVASLQARID
                     HHRLNDEIKIHGYLPYAEYLALRARCHAMVLTMYGQTWGQVATESVWSGLAAVLNDTA
                     TTLEVLPDGEASLVFKSGDPESLCAAIDRLYRDEALRCRLVRQAQSIVRLFSWDRYVD
                     AMEDLFVRVRGRSKLAK"
     gene            4443..4841
                     /locus_tag="VFQ61_19030"
     CDS             4443..4841
                     /locus_tag="VFQ61_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956604.1"
                     /translation="MFKAWICSAMFAASFALFGFALSVQQDRFTWTSYRDPLIWDAPP
                     IVIWIPTIEALEPDPAESETAEVSREIESVIYLPEVQISARRAQSGTARARGPLHTTP
                     YVQPYVQQVSDVCPPNQEKKTTSGLLTICG"
     gene            complement(4824..7055)
                     /locus_tag="VFQ61_19035"
     CDS             complement(4824..7055)
                     /locus_tag="VFQ61_19035"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9956605.1"
                     /translation="MSTTHENPKIRTVRTMCPMNCHPTHCGMRVELEGDRVVGIHGDP
                     ENPDSRGFLCVRGQACAEIIGNPARLTQPRLRRGFAASELYQRLLGSEATPPTRESPG
                     SIEADTALPESPDADSGWCTIDWERALELIARTIESAGRSRSAILTGHGVRTPLVARF
                     AHLLQTQYFNPSILCWGLGGFGFHLTGVTEVHSAEDLAAHAELVVLWGSNITSQPTTR
                     RALAAAKRRGAKLFVIDVRYSESCTLADEVFLVRPGTDTALALAIARILIDDQLYDHA
                     FVSAHTFGFEEFAQSLRELDLEQSIALTGIAPERVRALAHAYGSSRRAMILAGGSSMH
                     KTGNGWHAARAIACLPALTGSLSQPGAGMGPRHAAQCHGMGMAKWVPPDPRPPESVIP
                     DEMSRILEALESGAVRVLLLLGTNMLSSFADAGRLEKALRRLDLVVSFDLFENDTSRA
                     CAHLSLPGTAWLEELGFKATNTHLYLMDHFLEPPPGVRSAAWLLRQLADRLGCKDFFP
                     WSTMEQALDALFDHDATGHTTVARLRANDARQKLNVNPVAHPEREYSTPSKKVEFVSQ
                     RAVELGLPALPTLPPNTENSPTSALASRYPLVLTQGRTLTHFHSFYDHGRALPKLARA
                     DPEPRLWLNPQDAVERHIGDDAWLRVYNDRGELRTRARVTNQVPRGVVYMRDGWLGLN
                     HLTSSARSVPDAAARAFPAGSAAYEARVEVEALPFEETAPPQPTNAAAKEPARAELST
                     TDG"
     gene            7621..7989
                     /locus_tag="VFQ61_19040"
     CDS             7621..7989
                     /locus_tag="VFQ61_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956606.1"
                     /translation="MRSLAILTAFAAACVTPPAPNGLELPAVPRQNCAGCQGTDDTSA
                     SARDLRFTRTAISISPMSMPSRHSAAKGGGASHVLHGAMAQIKSAMVAGADKNIYVEP
                     IIGTVEYARKKYKYPQERSN"
     gene            8500..9036
                     /locus_tag="VFQ61_19045"
     CDS             8500..9036
                     /locus_tag="VFQ61_19045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9956607.1"
                     /translation="MAGNDAARFSQLVSEHLDFLWRALRRFGVREADVDDATQRVFLI
                     ANEKLPRIEVGRERSFLVGVATRIAAHARRSYQRRDVAEGQLSTSPPADNPNPEELTQ
                     QLQARMLLDRVLDKMPEELRIVFVLFELEELTVDEITHLLGLPRGTVATRLRRSREVF
                     HDCARSVAAERSEGRKNG"
     gene            9029..9874
                     /locus_tag="VFQ61_19050"
     CDS             9029..9874
                     /locus_tag="VFQ61_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956608.1"
                     /translation="MADPLRPLEVRLLEEGADDFEAQLLSAGRRDAPRAESRRHILAG
                     LGVIAVASTITTAANAMARPSTAVAVEKPGILARLAGTGKGALAKWGVVSGAGALAVW
                     AGARLSHSEMPAQPSPRAAQSQPVGSAVTAVADPPQAAPAADSVEGTVEGTAEADNAA
                     SPSTVAGREATVRGNSAQSGTLSPGAQVPTAGNLTAELALLESSRRALVAGEPKRSLS
                     LLDEYTRKFPRPRLSSEAQVLRIEALVASGERGKALELGRSFLARQGHSPFARRVRSL
                     IGVEK"
     gene            9877..10908
                     /locus_tag="VFQ61_19055"
     CDS             9877..10908
                     /locus_tag="VFQ61_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956609.1"
                     /translation="MRPGVRVESSVRRVIGRVLWLALSGAGLGGGTSCQDEFSLGSWR
                     QLGGATSTAGAGASTGGARAGAGGVEGEGGSSGAAGAIDRLCGVDTEFSELNQAGPAI
                     KATQVTTSWIWPDVHDSFDGEIEIESEFTSDAYFWAHQFWFDAGSGGFLGLQARGAFQ
                     RDINSKIEITNMVVFWINGSTTFRAEPGDAPDSDIRIGSSTDASGSWTTINVKYPFLA
                     CHKYRLRVSRDEAVGDSVYWAASVTEVDSGTTLKVGRIAVPAVWGRLDTRSQMWSARL
                     AEILRPASSCSDLEPASAFFGLPSANEGAMRPSSKTTGVSSADLGCPSSRWGETSTGI
                     RHQLAVPRP"
     gene            complement(10937..12088)
                     /locus_tag="VFQ61_19060"
     CDS             complement(10937..12088)
                     /locus_tag="VFQ61_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956610.1"
                     /translation="MHRSCRAPRSWLTGFALCGAMLCESGRARADTEIIHVEYTADVA
                     CPSSPTFIARVLRSTPNVQLSDSRQGVRTFAVSIQRNRTSFIGKLVVLEGATATIARS
                     VAGEKCEPIAKALALSTVLAIDPTAAPQFDEDAPPNDENVDRNAAARGSDPSPASSSP
                     ATANSEEGLSREAPATREAPATKTGPSDPAPAVPPKARAPGERPPKEPGTANSRWLGL
                     FLGPSARSGVAPGLAFGGALSLESQTTGPLSGFGAEVAYFRASSDSVSGARSSLQYLL
                     AAPIGCGPKLELERLVRLAPCLSVELGIVGGKGEDLPLPRSDQRWWSAVKAPVHLSAP
                     LGSSVWLRAEAGPVFAITRYHFVFTDPRVSVYRVPTVAFFASLKLGFRL"
BASE COUNT         2257 a         3632 c         4021 g         2276 t
ORIGIN      
        1 ttgctccagg tgtgcttgca cgcctcgatg catagcttcg tgagatcacc aggcttgcgt
       61 ctatacgtgg atatcgaccg cgccgtcacc gatcgaaacc cgcagtggga gagcttcgtg
      121 caagaggccg tgcgcaccgg cgcgcccggg cgggtggggg tggccttggg ggtcgcgcaa
      181 gggctactcg ggaccccagt gccactcgac gtaatcgaag cgttgattcc ccgcgggtgg
      241 cgacgcgccg gactcgagcg cttgctcggg agagaaagtg tgctcggcgc aggtcgcaag
      301 ctgccggggg cgcgcacggt gctgctcgac gtgttgctcg acccgcgcgc gatgacgagc
      361 tgggcgcgtg acgtggtgct cccgccggag gactggctgc gcgagcactt tgggcgcggt
      421 agcgttcaga aggaaggcgt tgttcgccta catgctcgcc ggtacttgaa cctggcgcta
      481 cgttggcggc ctgaatgagg aagtgagatg cctctcgctc gaaaaacgga gctcaagatc
      541 aagcgctgca tcgatgtcgt cggcgcggct acgggactcg tcgtcttgtc accggtactg
      601 gccggggtgg cgcttgccat ccgggtgacc ctgggcgctc ccgtgctgtt ccggcaacag
      661 cggcctggtt tgggcgggcg cccattccag atgatcaaat tcagaacgat ggcccaagcc
      721 aagcctggtg agacttggta tcggaccgac gagcaaaggc tcacgaggct tgggcgcttc
      781 ctgcggcgca ccagtctcga cgagttgccc gagctcttct tggtgctgaa gggggacatg
      841 agcctggtcg ggcccaggcc attgttgatg gagtatctgg ccaagtacac gccggaggag
      901 atgcgccggc acgaaatggt tccgggcatc actggctacg cccaagtcaa cgggcgtcag
      961 aatactccgt tcagccagcg ccttcgggat gacgtgtggt acgtggatca cttcagcctg
     1021 aagctggacg gcgagattct gttcaaaaca ataggtctgg tcttccaacg cgagggcgtc
     1081 attccgggcc agaacgtgga cgacgtcgac gaccttggct tgaccgcgga tcgcgcgagg
     1141 gtttcggcat gattcacgaa ctgggagacg ttcggttgcg cttacccgaa ccgagcgaca
     1201 tcgatgcgct ataccggatc aagaacgacc cggagctggc cgcgctgctc ggcggacatg
     1261 gccgcggata ttcacgcgcc gatctcgccg attggatatc gtctcatcgg agccgacgcg
     1321 acgaggtgct gtgggtcgtc gtcgactccg catccgaaca atgcatcggt cacgtgggcc
     1381 tgtattgcat cgatcaccgg gtgcgcaaag ccgaattcgg aatcttgctc gagcgcggga
     1441 gttgggggcg cggcctcggg cggaggctca cgtcttttgc catcgagtat ggctttcgag
     1501 agctggcgct gtcacggatc gaactgaacc tcttggcgag taatgcgcgc gctctcaaaa
     1561 cgtacttggc ctgcggcttc gtggaggagg gacgcttgag gcgtgcgcaa ttcaagaatg
     1621 gccgtttcga ggatgtcgtc tggatgggct tgctggctga cgaatatcga gcaacctcct
     1681 cttgagggca gcggccggtg tcggcccgag taagcgtaac gactgactaa cggagcaccc
     1741 aaacttggca cgtatctatc tgtctcctcc cgacatgggc ccgcacgaac gctcgttgct
     1801 gctcgaggcc ttcgactcgg gttggattgc gccactcggg cctcatgtga atgccttcga
     1861 ggcagagttc gcccagcgtg tcggcgcgtc ccacgccgtg gcgctctcga gtggtaccgc
     1921 ggcgctccat ctggccttga ttctgttggg ggttcgagcc ggagacgaag tcatcacttc
     1981 tactctgacg tttgcggcta ccgccaacgc aattcgatac gtaggagcgg accctgtttt
     2041 catcgatgcc gaggcccgct cttggaactt ggatccggag ttgctcgaag ccgagctcga
     2101 ggccggagcg cgccacggac gtttgcccaa ggcggtgctg gccgtggatc tgtatgggca
     2161 actggcggac ttcgagcgaa tcgtgccgct ctgccagcgc tacggtgttc cgctgatcga
     2221 agacgctgcg gaggccctcg gggctcactc gaaggggcgc tccgccggca cgttcggtaa
     2281 gttcggcgcc ttctcgttca acggtaacaa gatcatcacg acgagtggcg ggggaatgct
     2341 ggtgaccgaa gatggggcgg tggcggaacg ggcccgatat ctggctaccc aggcgcgcga
     2401 tccggctccc cactatcaac attcggtggt tggttacaat taccgattga gcaatttgct
     2461 tgcggccgtg ggacgcgggc aactgcggga gctggaccgg aaagtcgcag ctcggaccca
     2521 cgtgcgacgg cgttacgagg ccgaattggg gtccctcccc ggactgtctt tcatgccgag
     2581 cgcggacttc aatcagccca atcactggct cacctgtgtg acgatcgatc cagcgcgctt
     2641 cggaagcgat cgggaagacg tgcggcgcgc actggagggg caggacatcg aggctcgccc
     2701 agtttggaaa cccatgcacc tccagccggt gttcgcgggg ttccgcgcga gagaacgcgg
     2761 tgtggcggct cggctcttcg aactcgggct ttgtctgcct agcggctcga acctggctga
     2821 gagcgaccag gcgcgcgtga tagaaatcgt gcgcgcccag tgtcgctcta gctaagtccc
     2881 catggggcga agcgtcagcc agtcatttcg caagcttgct gcgcccgcgg actctcacga
     2941 acagatcttc catcgcgtcc acgtaacgat cccagctgaa caggcgaacg atggactggg
     3001 cttgacggac caggcggcag cgcaaggcct cgtctctgta gagcctatca atcgcggcac
     3061 acaggctctc cgggtccccg ctcttgaata ccagtgacgc ttcaccatcc ggcaagacct
     3121 cgagcgtggt ggcggtgtcg ttcagcaccg cggccaggcc gctccatacg ctctcggttg
     3181 caacttggcc ccaagtctgc ccgtacatcg tcaaaaccat ggcgtggcaa cgcgcccgca
     3241 gagccaggta ctccgcatag ggaaggtatc catgaatctt gatttcatcg ttcaatcgat
     3301 ggtgatcgat tctggcttgc agtgaggcta cgtaggcggg gtcactcggc tctccgcaaa
     3361 tatcgaatgt cacgttcagg ccgcgactgc gcgccagggc gactgcttcg atggtctcgt
     3421 gaaaccggcg caggggcccg atgatgccgg ccgcaaagag ccggaatggc tcttccggtt
     3481 tgcgttcggt gggcgtgacg ggatccgtgc tgtgttccag tccccacggc actcgaaccg
     3541 cgtcgcgacc gagcacgcga cgccaccacc agcgattgtc ctcgtcgagc acggtgatga
     3601 ttccaggtgg gttgcgtacg gagacctgag tgagtagctg tcgcgggagg ctcttggcga
     3661 cctgtaccgc gagttgcgtc ttgtcctcgc ctcgccgcaa ggccgcgagt tggtaaaagg
     3721 gtgccggcaa atcgttacac atccagaccc aaggcacctc ggaacctgac gagtgcattg
     3781 cgttccacgt gggttgaggc agcgcaccgt gccagttcac gagatcgata tcggcgggaa
     3841 acagccgcgc catggactgg cccaagtctc gttcgcgctt tcgaaacgcg acgtgttgta
     3901 gcgttctgcc gaatatgctg gcggagcgcg agccatctgc gcgccgactc ccgtgcacgg
     3961 cgcgcacgtc caaaccctcc agatgtcggg gataacacgc ggccgcatcg taatagccgg
     4021 taatcacgtg gacttggtgg ccgcggccgc tcaggcgacg cgcgagttgc acgagctgca
     4081 attgttcgcc gccgggacgg tccagcaggc ggttgacgag gacgatgcgc atggggcgcg
     4141 agagtctagt cgttctcctg aagtcggcgc ggctttcgtc ggcgacagtt acagttccag
     4201 gtaaacaaat tatcaaagct gtatcaaatt gcccttgcca cggtaaaatt acagggtcat
     4261 gcttgcccga ggttaacaga ggagcgagtc gaggactcaa tcccgaagcg ggtttcagac
     4321 gcttcgtggg tcgggtgagg agtcactctc gctccgcgat tcgaaggtca actcggctgt
     4381 ttgcatgccg cctgactggc ggccgtgtcc gacgccacgc cgagcgtgga agtgagggca
     4441 agatgttcaa agcgtggatt tgtagcgcca tgtttgcggc gagcttcgca ttgttcgggt
     4501 tcgccctatc ggtacaacaa gaccgcttca cctggacgag ctaccgcgac cctctgattt
     4561 gggacgcgcc gccaatcgtg atctggattc caacgattga agctttggag cccgatcctg
     4621 cagagtcgga gacggcagag gtttctcgag aaatcgagtc ggtcatctat ctgcccgaag
     4681 tccagatcag cgcgcggcgc gctcagagcg gcacagctcg cgcgcgcggg cccttgcata
     4741 cgacgcctta cgtgcagccg tatgtgcaac aggtcagcga cgtgtgcccg cccaaccaag
     4801 agaagaagac gacgtctgga ctgttaacca tctgtggttg agagttcggc gcgggcgggc
     4861 tctttcgcgg cggcgttcgt gggttgggga ggcgcggttt cttcgaacgg gagcgcctcc
     4921 acttcgacgc gcgcttcgta cgcggccgag ccagcgggaa aggctcgcgc cgccgcatcg
     4981 ggcacggagc gcgcgctgct cgtgagatgg ttcaatccga gccagccatc tcgcatgtag
     5041 accacgccgc gcgggacctg gttcgtgacc ctcgcccgcg ttcgcaattc gccgcgatcg
     5101 ttgtatacgc gcaaccaagc atcgtcgccg atgtgacgct cgacggcatc ttgcggattg
     5161 agccataagc gaggctcggg atcggctcgt gcgagcttgg gtaacgcccg cccgtggtcg
     5221 tagaagctat gaaagtgcgt gagcgtacgc ccttgcgtga gcaccagggg gtaacggctc
     5281 gcgagcgccg aagtggggct gttttccgtg ttcggaggca gagtcggcaa cgcgggcaac
     5341 ccgagttcca cggcgcgttg cgacacgaac tcgaccttct tcgatggcgt cgagtactcc
     5401 cgctcgggat gcgccaccgg gttcacgttc agcttctgtc gtgcatcatt ggctcgaagt
     5461 cgagcgacgg tggtgtgtcc agtcgcgtcg tgatcgaaga gagcgtcgag ggcttgctcc
     5521 atcgtggacc aaggaaagaa atccttgcag ccgagtcggt ctgccaactg gcgcaggagc
     5581 caagccgcgg agcgcacgcc gggagggggt tcgaggaagt gatccatcaa atagaggtgc
     5641 gtgttcgtcg ccttgaagcc gagctcttcg agccaggccg ttccgggtag cgacaaatgg
     5701 gcgcaggcgc ggctagtgtc gttttcgaac aaatcgaagc tcacgaccaa gtccagccgg
     5761 cgcagggcct tctccagccg tccggcgtcg gcaaagctcg agagcatgtt ggtcccgagc
     5821 aggaggagca cgcgcaccgc gccgctctcg agggcttcga gaatgcggct catctcatcg
     5881 gggatgaccg actcgggagg gcgcggatcc ggcggcaccc atttggccat tcccattcca
     5941 tggcactgcg cggcgtgccg gggacccatc cccgcgccag gttgggagag gcttcccgtc
     6001 agcgcgggca agcaggcgat cgcccgtgcg gcatgccagc cgttgccggt cttgtgcatc
     6061 gaagagcctc ccgccaggat catggcgcgc cgactcgaac cgtatgcatg ggccagggct
     6121 cgaactcgct ctggtgcgat gccggtcaac gcgatgctct gctccagatc cagttcgcgg
     6181 agggattggg cgaactcctc gaacccgaac gtatgggcgg aaacgaaggc gtgatcgtaa
     6241 agttggtcat cgatcaagat gcgagcgatc gcgagcgcca gcgcggtgtc cgtgccgggt
     6301 ctcaccagga acacctcgtc ggccagggtg caggactcgc tgtagcgcac gtcgatcacg
     6361 aacagcttgg cgccgcgccg cttggctgcc gccaaagctc ggcgtgtcgt gggttggctc
     6421 gtgatgttgc taccccaaag cacgacgagc tctgcgtggg ccgccagatc ctccgcgctg
     6481 tgaacctcgg tcaccccggt gaggtggaaa ccgaatccac ccaatcccca gcacaaaata
     6541 ctaggattga aatattgagt ttgaagaagg tgcgcaaatc gcgccaccag cggcgtgcgc
     6601 acgccgtgtc ccgtaaggat cgcgctgcgc gaccgtcctg cagattcgat tgtacgagcg
     6661 atcaactcca gggctcgttc ccagtcgatg gtgcaccaac ccgaatccgc atctgggctc
     6721 tcgggcagcg ccgtgtctgc ctcgatcgag cccggggact cgcgcgtcgg cggcgtggcc
     6781 tctgagccga ggagacgttg atatagctct gaagcggcaa agccccgacg caggcgaggt
     6841 tgcgttagcc gcgccggatt gccgatgatc tcggcacaag cctggcctcg cacgcacaag
     6901 aacccgcggc tgtcggggtt ttctggatcc ccgtgaatgc cgaccacccg gtctccctcg
     6961 agctccacgc gcatcccgca atgggtaggg tggcagttca tcgggcacat cgtacgcacg
     7021 gttcggattt tcggattttc gtgtgtggtg gacatcgacg ggcgttctcg gggttctgtt
     7081 ctcgcgcgtt acttccttgg agcggatctg ggagtgctgt tcgttttggt tgcggggttg
     7141 cggggagcga ttctcgcttg gttggactct tctggagatt cggatcttac gaggcagtca
     7201 gagcggcttg gtggagcacc ccacgtttgc ttagcacgaa gaattttccg cttgctcaaa
     7261 agaaatccca agaatctaag cccggcagcg caaatatgag ctccggcgtg cgggcatgaa
     7321 ctcaatgcag gcggcagcac gcggggcatg caaatcgtcg gtcgtagaag ctccggccgt
     7381 ataagcaaac accaggagaa aagcaccctg cggtcaacaa ctgcccggaa agaagctgcg
     7441 cggcagaaag cccaaaatcg gagagctcgg gagccagctt cggccggcga tgggcaaacc
     7501 cagcatagga ccgggcaacc ggagcgtcga cccgagcggc gtaggtggca ggtcggcatc
     7561 aatgccgaga cgccaccgga tcggtggaag tcggggcggg ctcgtctcag ggaactcgga
     7621 atgagaagtt tggcaatact gactgcattt gcggccgcct gcgtcacccc accggcgccg
     7681 aacgggctcg aactgcccgc cgtaccgcga cagaactgcg ccggctgtca gggcaccgac
     7741 gatacgtccg cgagtgcgcg cgacctccga ttcacgcgga cggcaatctc gatctcgccc
     7801 atgagtatgc cctcacgtca ttccgccgca aagggaggag gagcgtcgca cgttcttcac
     7861 ggcgccatgg cccaaatcaa gtccgccatg gtcgcgggtg ccgacaagaa catctatgtg
     7921 gaaccaatca tagggacggt cgaatacgcc cgaaagaaat acaagtaccc tcaggagcga
     7981 tccaactgac agagcgctca cggagccgta aagagtcgca aatgagcacg gcgcgaccca
     8041 gcgcagagac ccagcgaaga gacccagcgc agagacccgg tccaacccac ccatgcacca
     8101 ctcgctcagc cgtggtgtct tgacgtggga gcacggttcg ggtgaccggg ctgggagcgt
     8161 cggtgctact ctgtgcaggt tacgttgcga ggcggagtgc gagtgcgttc aaaagcgacc
     8221 cgaatcagag tcgacgcgag tcattggcag cgctagacat caaccagtcg acctcggttt
     8281 tgtccgatca aactcatggc gtttagctcc agcgcacgcg gccctgtccc gtccgaacca
     8341 ttctcggatc gggcggattc gttcgttccc agttcgagtg gttcggcaac tggtttcccg
     8401 agtgttccgg aagccagctc cacgcgttcc agtgctccgc cgcgaatgga gtcggctccc
     8461 gcgagtcaga gctctccttc cgtgcagcga ggctcagaaa tggccgggaa cgatgcggca
     8521 cggttcagtc aattagtatc cgaacacctc gatttcttgt ggcgagcgtt acggcgcttc
     8581 ggcgtgcggg aggcggacgt cgatgacgcc acccagcgcg tgttcctaat tgccaacgag
     8641 aagctgccgc gcatcgaagt tggcagggaa cgctcctttc tcgtgggcgt cgcgacgcga
     8701 atcgcggcgc atgctcgcag gtcctatcag cgccgcgacg tcgcagaggg ccaactctct
     8761 acgtcgcccc cggcggacaa cccaaacccg gaagaactca cgcagcagct gcaggcaaga
     8821 atgctgctcg atcgggtact ggacaagatg ccggaggagt tgcgcatcgt gttcgtgctc
     8881 ttcgagctgg aagaactgac tgtggacgag attacgcatt tgcttggcct gcctcgcggc
     8941 acagtagcga cgcgactacg tcgctcgcgt gaggtgtttc atgattgcgc ccgcagcgtt
     9001 gctgccgagc gctccgaagg tcgcaaaaat ggctgaccca ctccgcccat tagaagtccg
     9061 cctattagaa gaaggtgccg acgatttcga agcccagctg ctttccgcgg ggcgcaggga
     9121 tgcgccgcgt gccgaaagtc gacgtcacat tctggccggg cttggcgtca tcgcggtcgc
     9181 gtccacgatc actacggcgg ccaacgcgat ggcacgtccg agcaccgccg tggcggtcga
     9241 gaaacccggc atcttggcgc ggttggccgg caccgggaag ggagcgctcg cgaagtgggg
     9301 agtggtgagc ggggcaggcg cgctggcggt ttgggcggga gcgcgcctct ctcattcgga
     9361 gatgccagca caaccctcgc cccgggccgc tcaaagtcag ccggtcggat ccgccgttac
     9421 ggcggtagcc gacccgcccc aggccgcacc agccgcagac tctgtggagg gcactgtgga
     9481 gggcactgcg gaagcagata acgccgcgtc gccgagcact gttgccggaa gggaagcgac
     9541 cgtgcgagga aacagcgcgc agtcgggaac tctatcgccc ggagcacagg ttccaacggc
     9601 aggcaacttg actgccgaac tcgctctact cgagagctcg aggcgcgcac tcgttgccgg
     9661 cgaaccgaaa cgttccttga gcctactcga cgagtacact cggaagttcc ctcggccccg
     9721 tttgtcgtct gaagcccaag tgctcagaat cgaggccctc gttgcctccg gagaacgtgg
     9781 caaggcgctc gagcttggtc gaagcttcct ggctcgacag ggccatagtc cgttcgcgag
     9841 gcgcgtccgc tctttgatcg gcgtcgagaa gtagtcgtgc ggcccggggt gcgagtcgaa
     9901 tcctccgtac gtcgcgtgat tggaagggtg ctttggctgg cgctgagcgg cgccgggctt
     9961 gggggtggca cgtcgtgcca ggatgagttc tcattgggtt catggcgcca gctcggaggt
    10021 gctacttcca cggccggcgc gggagcttcg acaggcggcg cgcgggcggg agcgggcggt
    10081 gtagaaggcg aaggcggtag ctctggcgca gcgggcgcca tcgatcggtt gtgcggggtg
    10141 gatacggagt tttcggagtt gaatcaggcg ggccccgcga tcaaagccac tcaggtcacg
    10201 acgagttgga tttggccgga cgtgcatgat tcgttcgacg gcgagatcga gatcgaatcg
    10261 gagttcacga gtgacgcata cttctgggcg caccaattct ggttcgacgc agggtcgggt
    10321 ggctttttgg gcctgcaggc ccgcggtgcg tttcagcgcg acatcaactc gaagatcgaa
    10381 atcacgaaca tggtggtctt ctggatcaat gggtcgacca cctttagggc cgaaccgggg
    10441 gacgcgccag attcggatat ccgcattgga agctctactg acgcgagtgg ctcgtggacg
    10501 accatcaatg tcaaatatcc attcttggcc tgccacaagt accgactgcg cgtgagccga
    10561 gacgaagcgg tcggtgattc ggtatattgg gcggcctctg ttactgaggt agattcggga
    10621 acgacgttga aggttggacg catcgccgtt ccggcagttt gggggcgcct ggacaccagg
    10681 agccaaatgt ggtcggcgcg cttggcggag atcttgagac cagctagttc ctgcagtgac
    10741 ctcgagcccg cgtcggcctt cttcggctta ccgagcgcca acgagggggc catgcgcccg
    10801 agctcgaaga ctactggcgt gagcagtgcg gatctcggtt gccccagctc gcggtgggga
    10861 gaaacatcga ccggaattcg acatcagctg gcggtgccgc gcccttgagc tccggacgct
    10921 cggcgcctcg gtgtcctcaa aggcgaaacc ccagcttgag cgaagcgaag aaggccaccg
    10981 tcggtacgcg atatacgctt actcgtggat ccgtgaacac gaagtggtag cgggtaatcg
    11041 caaagacggg ccccgcttcg gcgcgaagcc ataccgagct gccgagcgga gcggaaagat
    11101 gcacgggcgc tttgacggcc gaccaccatc tctgatccga gcgggggagc ggcaagtctt
    11161 cgcctttgcc acccacgata ccgagctcga cggacaagca tggtgcgagc cggaccaatc
    11221 tctccaactc tagtttcgga ccgcaaccaa tcggagccgc gagcaagtac tggagtgaag
    11281 aacgggcacc cgatacggag tccgagctag ctcggaagta agctacttcg gcaccgaatc
    11341 cgctgagggg ccctgtcgtt tgcgattcca aactgagcgc gccgccgaac gcaagcccgg
    11401 gcgcaacgcc acttcgagca cttgggccca aaaacagccc taaccagcgc gagttcgccg
    11461 tccctggctc tttgggtggg cgttctccag gtgctctggc ctttggcggt accgcgggcg
    11521 cgggatccga ggggccagtc ttcgtagccg gggcttcccg cgtagccggc gcttcccggg
    11581 atagtccctc ttcagagttt gccgtggctg gcgagcttga ggcgggggag gggtcagagc
    11641 ctcgcgccgc cgcatttcgg tcaacgttct cgtcgttggg gggagcatcc tcgtcgaact
    11701 ggggagccgc agtcggatcg atcgcgagaa cggtcgagag tgctaaagcc ttggcgatcg
    11761 gttcgcattt ttcgcctgcc acggagcgag cgatcgtcgc ggtcgccccc tccaatacga
    11821 ccagttttcc aatgaatgac gtgcggttgc gctggatgct tacggcaaac gtgcgtacgc
    11881 cctgccggct gtccgacagc tgtacgttgg gcgtcgagcg aagcacgcgt gcgatgaaag
    11941 tcggactgct cgggcaagca acgtccgccg tatactccac atggatgatt tccgtgtccg
    12001 cgcgcgcgcg gcccgactcg cacagcatgg ctccgcagag cgcgaagccg gtgagccagg
    12061 agcggggggc tctgcacgaa cgatgcatga atcgagccca ccatagcgtg ctctggcccc
    12121 ggtacgaagg cgcgatggca cagaatttcc cgatttaggt ggtcgctcct ggtcggctgt
    12181 accgtt
//
LOCUS       DASTKU010000549         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_106776, whole genome shotgun sequence.
ACCESSION   DASTKU010000549 DASTKU010000000
VERSION     DASTKU010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106776"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2963
                     /locus_tag="VFQ61_19065"
     CDS             <1..>2963
                     /locus_tag="VFQ61_19065"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9956611.1"
                     /translation="VAVHAAIRPYGGHLQLIRADGFDVVFGAPRAQEDHAKRAILAAQ
                     ALQSGWADTRGSRLEGGGTSLALGLGIHSGRVVVRSGPDGTTPLLVGAVSLLAAQLAQ
                     NADGETPLLSGDTAELVRSFVDLRQLTELLVPGRAQALTTFQIVGQSTPRLRLASEAR
                     HSGPFVGRRSELEFLREHLRRARGGAGQAVSIVAPPGMGKSRLLAEFQSELEHGPERG
                     GVTTLCGRCQSYGQHIPYLPFVDLLRDWWGIGEREGIHSTRGKAIQGLSNAGMTSSEV
                     VHSVLDLLGAPGESLGAKQPAQLRAQTFGALHRLFLDASEQKPLVLMIEDLHWADATS
                     AQYLSSLAGQLGGHRLLLLTTFRPEYRPPWYGGGAERSKPSASVHVQLALAPLGRAES
                     EQVVGALGQGALSAQLSARIIERSEGNPFFLTALTQAVLTPDSDGVTSGVPNTIQSVL
                     GARIDRLSPLAKQALQVAAVIGADVPRALLEAVTAQASEPFGKALAELEASELLYEAQ
                     LSPESRYAFQHSLVEDVAYQSLLRKTRQELHRRILFAQLEMLNRHGALDSSPLSDRGE
                     RIAYHAVRGEVWDKAADFCQQAGTRAFAHGSNAEAASYFEQAISALDRLPRNRDTITR
                     AIDLRLSLRSALLPSSDLERILACLREAEVLAESLGDSRRLAQIAFFLSLQLYLGGQH
                     RPAAAAGRRAQLLSEGTENAVVHALATYLIGIPTQALGEYRSAIACFERTLAALEGPK
                     RHEFFQLAILPSVTCCAFHATCAAELGDFPSARVKGLEGVRIADAVAHRPTQMFAAWG
                     AGWVAFRQGDAQTALEFLERALGICRESELMLHFAMVAVPLGSTYLLAGRAEAAVALL
                     EETLAHVVARKLVNYHGVCSYSLAEALLETGKLEEARAHAERAIELTQKQGERGYEAY
                     AWRALGDISARGGEPVRALECYRSSLNVAQELQMRPLQARCWAALEVTERSLGHAAEA
                     RACRERADQLFADLG"
BASE COUNT          501 a          967 c          978 g          517 t
ORIGIN      
        1 aggtggcagt ccacgccgcg attcggccgt acgggggcca tctgcagctg atacgagcgg
       61 acggcttcga cgtggtattt ggcgcaccac gagctcagga ggaccacgcg aagcgcgcga
      121 tccttgcagc gcaagcgctg caaagtggct gggctgacac gcgcggctca cgtttggaag
      181 gcggcggaac ttcgctcgcg ctggggctcg ggatccattc ggggcgcgtc gtggttcgct
      241 ccggtcccga cggcaccact cccctgctgg tcggggcggt atcattgctc gcggctcagc
      301 tcgcccagaa tgcagatggg gaaaccccgc tactgagcgg tgacaccgcg gagctcgtgc
      361 gtagcttcgt ggacctgagg caattgaccg agctcttggt accgggtcgc gcccaagccc
      421 taaccacgtt ccagatcgtt ggtcaatcaa cgccgcgtct gcggctcgcc agcgaagcac
      481 gacactccgg accgttcgtg ggtcgccgaa gcgagctcga gttcctacgt gagcatttac
      541 ggcgagcccg cggcggcgcc ggtcaagcgg tgagcatcgt cgcgccaccc ggtatgggta
      601 agtcgcggct tctcgccgaa ttccagagtg agctcgagca tggccccgaa cgcggcggag
      661 tgacaacgct ctgcggccgc tgccagtcct atggtcaaca catcccgtac ctgccattcg
      721 tggacttgtt gcgggattgg tggggaatcg gcgagagaga aggcatccac agcaccagag
      781 gcaaggccat acaagggcta tccaacgcgg gcatgacgtc cagtgaagtg gtgcactccg
      841 tcctcgattt actgggtgcg cccggggagt cgcttggcgc aaaacaaccc gcccagctgc
      901 gcgcgcagac cttcggcgcg ctccaccgac tctttctgga tgctagtgag cagaagccgt
      961 tggtcctgat gatcgaggac ctgcattggg ccgacgcgac gtcggcgcag tacctcagct
     1021 cgctcgcagg ccagctcggc gggcatcgcc tgctgctgct cacgacgttc cgtccggagt
     1081 atcgccctcc ttggtatggc ggcggggccg agcgaagcaa gccgagcgcg tccgtgcatg
     1141 tacagcttgc cctcgcgccg ctcggccgag cggagagcga gcaggtggtc ggcgcgctcg
     1201 ggcaaggcgc gctgagcgcc cagctgtcag cgcgcatcat cgagagatca gagggaaatc
     1261 cgttcttcct gactgcgctc acgcaagccg tgctgacgcc cgactcggac ggtgtgacgt
     1321 cgggtgtacc gaatacgatc cagtccgtac tcggagcccg catcgaccgc ctatcgccct
     1381 tggccaaaca ggctctgcag gttgcggcgg tgatcggcgc ggacgtgccg cgcgccctgc
     1441 tcgaggcggt gaccgcgcag gcgtccgaac cgttcggcaa ggcgctggcg gagctcgaag
     1501 ccagcgagtt gctttacgag gcacagctct ctccggagtc gaggtacgca ttccagcaca
     1561 gcttggtcga ggatgtcgcc tatcaatcct tgctgcgcaa gacgcgtcag gaactgcatc
     1621 gacgcatcct gtttgctcag ctcgaaatgc tgaaccgtca cggagctttg gattcgagcc
     1681 cgctgagtga tcgcggagag cgaattgcct accacgcggt gcgcggcgaa gtttgggaca
     1741 aagccgccga tttctgccaa caggcgggca cgcgcgcctt cgcccacggc tcgaacgcgg
     1801 aagccgcgag ctatttcgag caggcgattt cggcgctcga tcggctccct cgaaatcggg
     1861 acacgatcac gcgtgccatc gatctccgac tctcgctgcg gtcggcgcta ctaccgtcca
     1921 gtgatctcga gaggatcttg gcgtgcctgc gtgaggccga ggtgctcgct gaatctcttg
     1981 gcgattctcg aagactggct cagatcgcct tctttctgtc gctccagctc tatttgggcg
     2041 ggcaacaccg cccggccgcc gccgccgggc ggcgcgcgca gttgctcagc gaaggcaccg
     2101 agaacgcggt ggtgcacgcg ctcgccacct acctaattgg catccccacg caggcgctgg
     2161 gcgagtaccg atcggcaatt gcctgcttcg aacgtacgct ggccgcgctg gaagggccca
     2221 aacggcacga gttctttcaa ctcgcgatct taccgtccgt cacgtgctgc gccttccacg
     2281 ccacctgcgc agcggagctc ggggacttcc cgagtgctcg ggtcaaaggt ttggaagggg
     2341 tccgtattgc ggacgcagtg gcgcaccgcc ccacgcaaat gttcgccgct tggggcgcgg
     2401 gctgggtcgc cttccgccaa ggcgacgccc aaaccgcgct ggaatttctg gagcgcgcac
     2461 tcgggatctg ccgggaatcc gagctcatgc tccacttcgc aatggtggcc gtgccactcg
     2521 gttcgaccta tctgctcgcg ggacgcgcgg aggctgccgt tgccctgctc gaggaaacgt
     2581 tggcgcacgt ggtcgcccgc aagctcgtca actatcacgg cgtgtgctcg tactcgttgg
     2641 cagaagcgct gctcgagacc ggcaaactgg aagaggcccg cgcgcacgcc gagcgggcga
     2701 tcgagctcac gcaaaagcaa ggcgagcgcg gctacgaagc ctatgcctgg cgagccttgg
     2761 gagacatctc ggcccgcgga ggcgagccgg ttcgagccct ggaatgttac cgaagctccc
     2821 tgaacgtcgc ccaggagctg cagatgcgtc ccctccaggc ccgctgctgg gcggccctcg
     2881 aggtcacgga gcggtccctc ggtcacgccg cagaagcgcg cgcgtgccgt gagcgtgccg
     2941 accaactctt cgccgatctc ggg
//
LOCUS       DASTKU010000550         4049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1892761, whole genome shotgun sequence.
ACCESSION   DASTKU010000550 DASTKU010000000
VERSION     DASTKU010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892761"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFQ61_19070"
     CDS             complement(<1..303)
                     /locus_tag="VFQ61_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956612.1"
                     /translation="MGFASSTGVELGSSYGFTWCGGPCPPPTEQCSFFAREHTSSEQL
                     GLLEALRLAASPACRYADGWFSYGLTVMDADGETTTYSGPSCGPSTGPRDGTVPKEF"
     gene            complement(1711..2151)
                     /locus_tag="VFQ61_19075"
     CDS             complement(1711..2151)
                     /locus_tag="VFQ61_19075"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9956613.1"
                     /translation="MQIHEVYPYLRVRNAAAAIDFYKRTFNAREKFRLTEPSGRIGHA
                     ELEFGSVVIMVSEEYPEYQIFGPESVGGTSVSIHLHVDNCDELIAHAVNMGAKLVRSP
                     TDQFYGERSGTVRDPFGHEWLIGHNIEDVTPEEMQRRYTKLMTP"
     gene            complement(2154..3158)
                     /locus_tag="VFQ61_19080"
     CDS             complement(2154..3158)
                     /locus_tag="VFQ61_19080"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9956614.1"
                     /translation="MPVFSRRPCAALAPWVQQLWATHPEATQSSAAYRESVLPTGAMH
                     LVLRGSDSPPLMLFDGAAGERSHSIGHLIVGGARSSYYVKQAGDGARSVGVQLQPGGA
                     FALLGVPASELAERHTPLDLLWGAEAARVREQIYDLPRRSFFKACPADIHAWMREQVD
                     LFESFLLQRLRQANTDEPQRLWLAKIIQALERGVPLAAIAESSNISHRTFITRFRSWV
                     GLPPKVFARIRRFQRAITLLNRTSAASYRRAPGSLAQLALETGYADQAHLSRDFVEFA
                     GVSPSAYSKLAPAAPNHVPVQLLSKALNTTGSNSFKNPARVRGRFEPEPRQGDLEEGF
                     "
     gene            complement(3305..>4049)
                     /locus_tag="VFQ61_19085"
     CDS             complement(3305..>4049)
                     /locus_tag="VFQ61_19085"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9956615.1"
                     /translation="LVREVEQRIQPGGGTPTTSKCTKPSAPSLTDDQVKANAQLPTVG
                     KTVMDLLATGLACDTTRVASIVWSPGESFYNFAEILPNLPWKDLSCPPNVDTCTDGKN
                     LPATFLHVMSHFPPSTANGVPGNMNTVQKAALECLTAVEVWFNERLAYFAQALAAVEE
                     EDGSNLLDNTVLLSVKDISEGVTHGYRDVPMVLLGGKGLFKPGHFNLQNRKTFGDLYA
                     TVAQALGFPEVTTFGDPEYFTGPITEIRG"
BASE COUNT          759 a         1197 c         1343 g          750 t
ORIGIN      
        1 gaactccttc ggaactgtcc catcccgcgg acccgttgag ggcccgcaac tcggacccga
       61 ataagtggtc gtctctccgt ctgcgtccat caccgtcagg ccataggaga accaaccgtc
      121 cgcgtaccga cacgccggac tagcagcgag cctgagcgcc tcgagcagcc ccagctgctc
      181 actcgacgtg tgttcgcgcg caaagaagct gcactgctcg gtcggcggag ggcacggccc
      241 gccacaccag gtgaagccgt acgaggaccc gagttccaca cccgtgctag acgcgaaccc
      301 cacgcgtgcg ggatcatccg gtggtccctc aggggtgtca ttctctgagg ccccggcgtt
      361 cgaagcagcc gcagaatcga cagacccttt acctgcggtt gcgattcacc ccgcggcccc
      421 cttcctttgc gccgatcgaa attggttgga gcccaggatg acacaatccg gcacacgttg
      481 aatcgacgtc actgtggccg cgggtaggct tggccgcacc tggtaccgtc gattcagcgg
      541 gcgacgcccc ttccgcgcca tcttgattcg gcgatgtgtt tgttcgacgc ggctgaacgg
      601 agcccgtggg gcggcgtttg aggcggcagg tcgtggctcc caccaagcgc aagcgttgct
      661 cggaacatta gccccttctt aggctgccgg cgacgcccct ttcgcgccaa cttgatttgg
      721 cgatgttgtt cgacttacga gaacgagcgt gcgcttcgtg cagacaaaat ttgttgttta
      781 gtccggtgaa gagtgtcatg ctgagtgcaa ctcagcgcgc gccaaaacga agcgcgtgag
      841 ttcaacagag agaggggccc gaggtatgtg catacggaat gtggcacggg agcagttgag
      901 ccttaatttc cagagtgcgt ggggagggaa acgcgccggc gccggacgca agcgtcgggg
      961 cgattcgatg ccgcacacgc ggcgcggcgg ccaccgacgt tacgagccgg tgcacgttac
     1021 cgtccgctta ggagtgcggt gtttgaggcg gcagcgcgtg tacgcggtgg ttcggcgggc
     1081 gatcgccagg gtgaacgccc tgcgagggcg agaattccgg gtggtagagt tctcggtgca
     1141 gactaatcat atccacctcc tggtggaagc agagggccgg attgccctga gtcacggaat
     1201 gcgcgcgctg aatgcaagat tcggcaagca ggtgaatcgc gtgctggggc ggcgtgggcg
     1261 cttgatatcc gatcgtcacc acgagagggt gttgcgctgt ccgccatgct ttggtttatg
     1321 tactgggcaa cttcaggaag catcccacgc cggagggccg acgtggggag ctcacagatc
     1381 cgtgttcgtc ggcccgcttc ttcgacggct tcgcagaact gcagggcgca gggctgcggg
     1441 caacagagcg aaccgcggac tctccggtga ctcacgcgcg tacgtggctg ttacgccttg
     1501 gctggcgaag gcacgggctc atttccatct acgaagcgcc gcggggcgct tagagatcat
     1561 cccaggagga gagccggaga cggggcggag ccgtctcggc aaagtgaggg cctgcgcggt
     1621 gtggcgacgg ggcgcgcggg gctgcgacgg agcttgggag ctacgtgacg cgcggcgcgc
     1681 ccgtctacga ggtggcccga actcgagagt tcacggggtc atgagtttgg tataacgacg
     1741 ctgcatctcc tcgggggtta cgtcttcgat gttgtgacca atcagccatt cgtgaccaaa
     1801 aggatcgcgg acggtgccgg agcgctcccc gtagaactgg tcggtgggcg agcgaacgag
     1861 cttggcgccc atgttcacgg catgggcgat gagctcgtcg caattgtcca cgtgaagatg
     1921 aatggatacg ctcgtgccac ccaccgactc cggtccgaag atctgatatt cggggtactc
     1981 ctcggagacc atgatgacga cggatccgaa ctcgagctcg gcgtgtccga tgcgcccact
     2041 gggctccgtc aagcggaact tctcccgtgc gttgaaggtg cgcttgtaga agtcaatcgc
     2101 cgcagcggcg ttgcgcacgc gcaggtaggg atacacttcg tggatctgca tgctcaaaaa
     2161 ccttcttcta ggtcgccttg tcggggctcc ggctcgaatc tgccccgaac tcgcgcaggg
     2221 ttcttgaagg aatttgaacc cgtggtgttc agcgccttcg acaagagctg cactggcacg
     2281 tgattggggg cggccggcgc cagcttcgaa taggccgagg gagatacacc cgcaaactcg
     2341 acgaagtcgc ggctcaagtg ggcttggtcc gcgtagcccg tctcgagcgc gagttgcgcg
     2401 agcgagccgg gagctcttcg gtagctcgcc gcagatgttc ggttcaatag cgtgatagcg
     2461 cgttggaacc ggcggatgcg cgcgaatact ttgggtggca accccaccca gctgcgaaat
     2521 cgcgtgatga aggtgcggtg actgatattg ctactctcgg cgatcgcggc cagcggcacg
     2581 ccccgttcga gggcctggat gatttttgcc aaccacaggc gctggggctc atcggtgttg
     2641 gcctgacgca agcgctggag cagaaacgat tcgaagaggt cgacttgctc acgcatccac
     2701 gcatgaatgt ccgcagggca cgctttgaag aagctgcgcc gcggcaagtc gtatatctgt
     2761 tcgcgcacac gcgcagcctc cgctccccac agtagatcga gcggcgtatg ccgttcggcg
     2821 agttcgctcg cgggaacccc cagcagcgcg aatgcgccgc ccggttgcaa ctgtacgccc
     2881 accgaacgag cgccgtcgcc ggcttgcttg acgtaatagc tggagcgggc gccacctacg
     2941 atgaggtgcc caatagaatg ggagcgttca ccggccgcgc catcgaatag catcaaaggt
     3001 ggcgagtccg agccgcgcag caccaagtgc atggcccccg tcgggagcac gctttcccga
     3061 tacgcagcag acgattgcgt ggcctctggg tgcgtcgccc acagctgctg cacccagggc
     3121 gcgagcgctg cacagggacg acgagaaaag acgggcatga ccaggtcagg gtgagcgacc
     3181 tcacgagaaa gggcaaacca tggtcgaaaa gtcccaaacg cgcggcgcaa acgtcccctc
     3241 cctgccccat cgcgcaggag aagaaggatt ccggtcgaga accggatctg ctctcgacgc
     3301 cgagctagcc gcgaatttcc gtaatgggac cggtgaagta ttcggggtcg ccgaaggtcg
     3361 ttacctccgg gaagccgagc gcctgcgcca cggtcgcata gaggtcccca aaagtcttgc
     3421 gattctgcag gttgaaatgg ccgggcttga agagtccctt cccgccgagg aggaccatcg
     3481 gaacgtcgcg gtatccgtga gtcacgcctt cgctgatgtc cttcacggag agtaacaccg
     3541 tgttgtccag gaggttgctg ccgtcttctt cttcgaccgc agccagcgct tgggcgaagt
     3601 aggcgagccg ttcgttgaac cagacttcga cggccgtcag gcactcgaga gcggccttct
     3661 gcacggtatt catgttcccg ggcaccccgt ttgcggtcga tggcgggaag tgcgacatca
     3721 cgtgcaggaa cgtagccgga aggttcttac cgtccgtgca agtatcgaca ttgggtgggc
     3781 aggagagatc cttccacggc aagttcggca aaatctctgc gaagttgtag aacgattcgc
     3841 cgggtgacca gacgatcgac gcgacgcgcg tggtgtcaca ggcgagaccc gtggcgagca
     3901 agtccatcac ggttttaccg acggttggga gttgcgcgtt tgctttcacc tggtcgtcag
     3961 tgaggctcgg ggcagacggt ttggtgcact tggacgtcgt cggggtaccg ccgcccggtt
     4021 gaatgcgctg ctcgacctcg cggacgagc
//
LOCUS       DASTKU010000551        17858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1446780, whole genome shotgun sequence.
ACCESSION   DASTKU010000551 DASTKU010000000
VERSION     DASTKU010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 17858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17858
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446780"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="VFQ61_19090"
     CDS             <1..834
                     /locus_tag="VFQ61_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956616.1"
                     /translation="TALCFATPLLIACSSENPPIGGNAGAPPAAGGAVTVTGGASSSG
                     GATSSSSATAGASSASGGAPISGGSLSTGGVTSAAGGSHQTGGSADSSTGGTPIMGGS
                     TGSTNGGTSGASGGATNNQGGRGGTASLGGTNQGGNAGNGGRSSAGSGGIASDGGRMN
                     TGGGSGGSSGGANGGTGGNSSGTYAQVTKILETRCGNCHSTYLPQFFANSFEDKVSKC
                     SNHLTVNKGSPDTSALIESIEGRCSNLARMPRGCSGASCMPTSEVDLLKAWIQSGAPT
                     N"
     gene            987..1074
                     /locus_tag="VFQ61_19095"
     tRNA            987..1074
                     /locus_tag="VFQ61_19095"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1021..1023,aa:Ser,seq:gct)
     gene            1215..2912
                     /locus_tag="VFQ61_19100"
     CDS             1215..2912
                     /locus_tag="VFQ61_19100"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956617.1"
                     /translation="MKTLTFGVWWGRHALAFHIAIVLSCSVDKRRVFEEAGSGGASAV
                     AIAGTAGTSLAGNTTGGSLTSVGGHPNAHTEVENGGAAGSAGPDGNGEAAGGTMAFPE
                     NVGGTTSIGTRVCVIGSTEYQSGAPNPDNPCSECSPERSSSHWTLVDDGSACGSESVC
                     SSGSCKEGCWLGEALVAPGTANPDNPCLICDPSRSRTDWTRGSSSSCVSAITANELHT
                     CAVIDGAVSCWGKNQFGQLGDGSRNDSSRPVQVSALTSGVSAISAGGQHTCAQVNGSI
                     RCWGSNASGQLGNNSLVDSLQPVQVEGVYSRVIDFSAGYVHTCGIFEGAARCWGSNDM
                     AVTDRPDATRLGDGSNTDSPLPVEVQGLSSGVTKIVAGRIHSCAIANRTAWCWGMNVF
                     GNLGIASDTDSRVPVRVQGIDSSVTDISVGVYSTCAIAQGAAWCWGKNDEGQLGNGST
                     ASSSVPTRVNGLETGVEAIVAGYFHTCALVRAAVRCWGANDKGQLGNNSTKSSLVPVE
                     VDGLSSDVTALTAGAHHTCAIANGLARCWGANDVGQLGTERVSNSPVPIEVSFQSSL"
     gene            3055..5031
                     /locus_tag="VFQ61_19105"
     CDS             3055..5031
                     /locus_tag="VFQ61_19105"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9956618.1"
                     /translation="MKSPNEVWVRTSLGAAVLKCSVPGVTNTSEQVRLNSQVAWDLVH
                     LLNGAHGLDELRLARELESILPTGGLGPPSRAPSPRQVRLWLSGIAGRVADALRAGLL
                     RVELVAPSREGVWHDRVERQSEPDPVPQRSAPPPRERVTTWFSLRIIDEVGAPVEGVS
                     VKVSTQDGDQTLTTSSTGVVRLDDVSNSRSASARLVDQSQVRSVLSPRWREPRSRRNF
                     GGSSVLIRSLAEAFLDPLELPSEIPTTLVLTPHFECREIPGAYFEFGRSFVRAEALEP
                     LADIAEALRGDENLRALICGHTDISGSDALNKELSERRAKAVHALLTHDADAWEELFS
                     GTADGSHWQERWGVKEAQHMLNALGVTDDSGAPLVEHGERDTATVQAIHRFQRGDYPD
                     KPAEQAPLAESTTLGAEGRRELFLAYAKRVSRQPSARDRFTKVGSAPFMGCGEFNPVK
                     ATARDQESRRTVVFLFDPAAEPSGLPCQLRQLAPCKSNLEPASSTAQRERPYRCRIYQ
                     KVAERCPCQAGPDLSHDLVLRFPMTLASANTLPHRYILTSEDGTINQTRELASQARAN
                     EQQRAELTFEHLPDTHRYRLICDEPTSTYTLFELAALSELQERLATDGEVPEKDVPLE
                     LAGWFAEAEVDAPASDSQSAGDQTSLPPEDGASA"
     gene            5028..7970
                     /locus_tag="VFQ61_19110"
     CDS             5028..7970
                     /locus_tag="VFQ61_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956619.1"
                     /translation="MTQAVVQPFLPEEFERIAGTPTITLGTALYVIRATVFDPVRKLD
                     VAVPAHSWVLLAGAGSNARPFARSNASDSDGRAALMAQQSLPDGEYSLALVPFPSIIR
                     EGYLRDREAWIDLDRGDWALPPSAVANLERRRLVRIPAWSTMRKAASGGFKIVPPGSS
                     QFATLGILKAAEIQPFGSPQEPWAMPLDFNWVKAQVRFFFSNWQLNQTEVVPPGLLVE
                     AVSDRLPPRSNPKLGAGVALTPTDGSIYMLLEGDATLWRECQFQFVTPGSAYVDVRQK
                     ASAAPDLRLTTEQPVPEPSEDRNRLPSAWHSHGMRASFGDPSETQPFKKRSARWDDLR
                     KQISKDPSAPQASVFFHLDDVVLCDEAGQPIAVPSKHQPPTLFDHFLRVHEQNEKKPY
                     QSKVDFVGGGVIPAERAYPVGEKLTTRDKLLWNSTRLIHFEGAFYDLRDDRVTGRAGR
                     TTAVGARSARRGQHPHAVLSKGNPYTNYHGPTELHAIDVPGVTDPSTGTPLQHVLIYQ
                     SVIFERTPDFPESVLPEVYRNLYAAGERWSPGHPGVHGKIQQKSYALVSQEPARRAER
                     TTRIRTFLAETSSGAYITIVLKNSDQARSSYGDAWFLYGSRITYYSGAVRTDTAGIHS
                     MLDKGDSVELPWHTLAHEFGHALGLPDEYCEPLQVEGLPPKVTLTEPVLPSFNQSDRG
                     YGAYRPFNSDQAAMMNSNRLPRLRHFWHHVKILNEHPPFAQLPGIPYSLVHTKFGDSQ
                     LTYRVPPRDDHSPYAPIFSDAPIPGGHGHCAMWALGDDEGSVRSLFGLENAALMPDRM
                     QGLLIVRVRFRFIFAKGISLERAWEDLRNFHLKLCDSKQIPQVRFLLGGGEPFPRIAV
                     VIQPQYEIGPTLDPLDPADIVVCVHSSSSPPPLQLDRLHHYDPRLAVNPFLKDNPDSP
                     LDILESDLLLEPLLRHALGARTWLSGSGGTRKVNLDALAPADVTSLARLVEQKLGEPS
                     GRRYIGVY"
     gene            8133..9260
                     /locus_tag="VFQ61_19115"
     CDS             8133..9260
                     /locus_tag="VFQ61_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956620.1"
                     /translation="MKTPDFQGTLAGSFSYPYASSLRNSQVFVKRRALASLSRTPLKW
                     VRGGMDPNELGGPVTLVVSPNGQHALAASGVYTFNLTSRGIIGIRDIPDRELPHDLVA
                     LDSSKYTQGSASTWDGRPFYEPNRQGCALARRIEEDKYAAVIIQGPIWDRNTERWTET
                     YVTLEGGLRHGNPNDVDWRLYVDCKTAVAAIGGDRATVLALDNHLLQVYAPEGNPRGE
                     APILVAERNIGVVATHVSIVGSNILVIAPGHPEEGSGSRLLAFTAETRPIYSLSMPFE
                     VLQPAIAGSGTRVYLAGRGLAALDGGGVSWLYVSQEPIYASSFEDGSLAVAHGKRLEF
                     VNTSGTVEQAFDTEEPLLAPPAIAADGTVWAISGRAVYIAR"
     gene            9603..10286
                     /locus_tag="VFQ61_19120"
     CDS             9603..10286
                     /locus_tag="VFQ61_19120"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET9956621.1"
                     /translation="MIRARWQQLALPAGLIAVDLLSKAWAVAWLKADQPVDVDAGLQW
                     VLRVNREGLGSWSRALAFAADARVAGAVLYAGFALAFLAFQRGHWTKWQKVAILALIF
                     VTSPFLGITLNLVIPSLSTPLLTRLCGAALFGVLWWLSKPGLWRWATAFMVSAGLGNS
                     LGLLVHGEGIVDFIYSRWMTSLLNMGVFNIADLYFNLAALCLTAWLGRWAARNAANAT
                     TRLLRPAGA"
     gene            complement(10255..10500)
                     /locus_tag="VFQ61_19125"
     CDS             complement(10255..10500)
                     /locus_tag="VFQ61_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956622.1"
                     /translation="MSSTFGLRSSELDRYWKLDTYEAGLSMVMTARDEILGRYGQSGK
                     TIFVVGHASAGQVMIGLLQGRGGGSYPTTRQLDAAGV"
     gene            complement(10497..10667)
                     /locus_tag="VFQ61_19130"
     CDS             complement(10497..10667)
                     /locus_tag="VFQ61_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956623.1"
                     /translation="MVANTLDPSEITYENSETLTEFGLSRIAELTSFLSGMGAKRDAV
                     IVSPTKRTQRSS"
     gene            complement(11111..11548)
                     /locus_tag="VFQ61_19135"
     CDS             complement(11111..11548)
                     /locus_tag="VFQ61_19135"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9956624.1"
                     /translation="MMTQPPELFGRVTAILSDHKNLRQTIGRLRQMCALLEQRPPTLA
                     PEFEPSGLLEGLHEDLVAHFAAEESEGYFGTVEAERPALAAQISELKSQHAVFLKELG
                     NLREMAQTTERWLELSAGTRSLVEQLESHERAESQLLHGFLLC"
     gene            complement(12010..13446)
                     /locus_tag="VFQ61_19140"
     CDS             complement(12010..13446)
                     /locus_tag="VFQ61_19140"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9956625.1"
                     /translation="MNVKRRAFLRAIGAGIAALPFLRVVENAYAQSEGLALPQRFVGV
                     YHPHGVAAELFTRREGETETEFTLDYPDSPLQPFDDPATYGRSFKHQIVVVEGIDLLS
                     DTNGHNSAGTILTGSRIVGGPGGLPWNSSIDQFLAVENGLGAETLVTSVALAVGNKEL
                     SARETLSFGAGGVPLTKIIDPALAFDFLFKRAVVGKDPAAQAAAEHQRKLGLSLIDFV
                     SGDVKRLRARVGTFEEQKLDQHLEALRALEKKYQSFVRGGDCSLPARPPAFPQLERYN
                     GGEPYFDAITDWQIDLLGLALGCDITRFATLALNDLSYAGNPLGLPADNHAGVAHTYA
                     ASALGNSGRPTDNQDPETWRRLAKFNHYVYGKIARLMQRLDESGILDSTLIYASSDMG
                     NPAAHSTRNVPTVLAGGVNGKIRMGRRIQFRTDCPLGTGCEPNSGEYQTVPNNRLLVS
                     IAQAFGIEIDYFGNQGEFEFATGTLPGL"
     gene            complement(13443..15188)
                     /locus_tag="VFQ61_19145"
     CDS             complement(13443..15188)
                     /locus_tag="VFQ61_19145"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9956626.1"
                     /translation="MSRGSALIWSLVCGAAVLGACSSSAERSASAPASSFSNGSSSPT
                     GGTSTASAGATGTDFGGGNSSGGAGSSDADSALAHLPTRIRRLANVEYDTSVQVLLST
                     KRSLAGGPDFPPDLRQDGFTVNDTQRIDAVLVERLADAADTLVDEAKQNGTFARLAPC
                     ETAADAANCAKRFIVTFGAKVYRRPLDDEEIEALMGLYSVGAEAASFQDGIAHVTRGL
                     LQSAGFLYLTELGEGQPASDGTVRLTAHELAASLSYFLTSAPPDAELLQKASSGALFE
                     PDEREAQARRLFEANPAARDTVVRLVREWLGIDRIAESSKDSLVYPEFAEEKPKIVAE
                     SQDFVRAVAFESTGTVGELLGANWTVNSGPLSLYEVAGAGPLPGSSRLTGRIGILNQG
                     AFLATYANAHESHPIFRGVAVTRRVVCYPLDSPASFNLQVVPPAPDPSLTTRERFSVH
                     PRDSICAGCHDIVDPFGFSFEQFDGMGRYRERENGKQIDSAVRVAMDAEFDGPYADSN
                     ALAAALSDSEIVRECFARFMFRAASATGDSAATPGEREFVDAWRKIPEAARGNIVETL
                     IAYVKSPSFAVRGGP"
     gene            complement(15290..16969)
                     /locus_tag="VFQ61_19150"
     CDS             complement(15290..16969)
                     /locus_tag="VFQ61_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956627.1"
                     /translation="MPDQTGTSSGAVDVVTDEGLDVRAGSRRGFLRIGAVVVAGFVDA
                     CSSTDPIGTGTGGAPGNGGSAATGGATPVGSGGVAPGAGGSAVTGGASATGGVIGSGG
                     TSSSGGTTAQGGASAGGAPAAGGTPSGGASPAGGTSGGSPVTGGTSAGGAAAGSPAGG
                     STGGGSPGSAWVENRGKACMIPNISMGTANPMLPDPFKFINGTRISKKEDWACLRAEL
                     SALAQACIYGPKMPPPDKLEATFSGGKLTINMTVGSKTGSFSVTISGGGTEAKPVPCL
                     ITCGGSSLPSLSGVAKIDMPNNTISQESKTVGSGGLVNTLYGSMSAKSGSLIGWAWGL
                     SRIIDALEKCPQAGIDVRRIAVTGCSRNGKGALAMGAFDERVALTIPQEGGSGGPALW
                     RVSTAEKKAGQNIQEATEIVGEANWQGSDFKSYANSVDKLMADQHTVIALCAPRAVLV
                     IDNDIDWLGPVACYGGGAAAQLLFQGLGIKDRCGVSVAANHSHCQFPSAQQNYLNAFV
                     TRFLKGGMADTNVDDFHVSSSTSKLGKFDKAKWIDWDVPMLSGSLAWDPFA"
BASE COUNT         3426 a         5620 c         5423 g         3389 t
ORIGIN      
        1 accgctctct gttttgcaac tccgttgctc attgcgtgtt cgagcgagaa tcctccgatc
       61 gggggcaatg ctggagcccc cccggcagct ggcggtgccg taacggtgac gggcggcgca
      121 tcctcgagcg gcggagcaac gtcatcctca tctgcaacgg ccggagcctc ctcggcttcc
      181 gggggagccc caatctccgg aggctcactt tcaaccgggg gagtgacgtc cgctgcaggc
      241 ggctcacatc aaaccggagg cagcgccgac tcgagcaccg gcggcacccc gatcatgggc
      301 ggcagcaccg gctctacgaa cggcggaacc tcgggcgcct caggcggagc tacgaacaac
      361 caaggcggtc gcggcggcac cgcatcactt ggtggaacca atcaaggtgg caacgcaggt
      421 aacggcggta gaagctctgc tggttcgggc ggaatcgcca gcgacggcgg gcggatgaac
      481 accgggggtg ggtctggcgg ttcatcaggc ggcgccaacg gcggcacggg cggaaactcc
      541 tcgggaacct acgctcaagt caccaagatc ctcgagaccc gctgcggcaa ctgtcattcg
      601 acgtacctcc cacagttctt cgccaactcc ttcgaggaca aagtctcgaa gtgctcgaat
      661 cacctaaccg tgaacaaagg ctcccccgac accagcgcat tgatcgagag cattgaaggt
      721 cgatgcagca acctcgcgcg aatgcccagg ggttgttctg gagcgagctg catgcccacc
      781 tctgaggtcg accttctcaa ggcgtggatc cagagcggcg ctccgaccaa ctagagcggc
      841 gctcttgtcg gctgaacgcg gcggtaccgc tcgaacggct caacggccgc acgatccttg
      901 gcaggatccg acacaggaaa cgcaacctag cccttgacgg gcgctgcagg ccgactaacg
      961 tggcgcgtcc gcaacgagga aaccttggag aggtgaccga gtggccgaag gtacccgctt
     1021 gctaagcggg cgtaggtcaa aagcctaccg agggttcgaa tccctccctc tccgcaaaga
     1081 atctagctgt tttcctgctg gatcagttca acggctgtga gtcgttggcc aaagttcgcc
     1141 ggcgctgagt tcggtgtgtg ggtgcgtggg cattcgctta gacgaatgct acggtcggtc
     1201 ggggtttgca aaagatgaag acgctgacgt tcggtgtttg gtgggggcgg cacgcattgg
     1261 ccttccacat tgcgatcgtt cttagctgca gcgtagacaa gcggcgggtg tttgaagaag
     1321 ccggcagtgg tggggcctcc gcggtggcga ttgctgggac cgcgggcact tcactggcag
     1381 ggaatacgac gggcggctca ctaacgagtg ttggtggtca cccaaatgct cacaccgaag
     1441 tcgagaatgg cggcgcggcc ggaagtgctg gccccgatgg caatggcgag gcggccgggg
     1501 gaaccatggc gttccccgag aacgtcggcg gaaccacttc cataggcacg cgcgtttgcg
     1561 tgattggatc taccgaatat cagagcggcg ctccgaaccc ggataatcca tgttcggagt
     1621 gctcgcccga gcggtcttcg tcacactgga cgctcgtcga tgatggaagc gcctgtggtt
     1681 ccgagagcgt ctgcagcagc ggctcctgta aggaaggctg ttggctcggt gaagccttgg
     1741 tcgcgcccgg aaccgcgaat cctgacaatc cctgcctgat ctgcgatcct tcgcgctcca
     1801 ggacggactg gaccaggggc agttcttcga gttgtgtatc tgcgattacg gccaatgagc
     1861 tgcatacctg cgcggtcatc gacggtgccg taagttgttg ggggaagaac caatttggtc
     1921 agctcgggga tggctcacgg aatgacagct cgcgtcccgt tcaggtatcg gcgctcacgt
     1981 cgggcgtgtc tgccatctcc gccggaggcc agcatacgtg cgcacaggtg aatggctcga
     2041 tcagatgttg gggctcgaac gcttcagggc aactcgggaa caactcattg gttgacagcc
     2101 tgcaaccggt gcaagtggaa ggcgtgtatt cacgggttat cgacttctcc gcgggctacg
     2161 tgcacacctg cgggatattc gaaggtgcgg cacgttgctg gggaagcaac gacatggctg
     2221 tgacggacag accggacgcg acgcgactcg gtgatggctc caacacggat agcccacttc
     2281 cggtcgaggt ccaggggctc tcgtcgggcg taacgaagat tgtggcgggt cgaattcaca
     2341 gttgcgccat cgccaatcgg accgcctggt gttggggcat gaacgtattc ggcaatctcg
     2401 gaattgcttc ggacacagac agtcgcgtac cggttcgagt tcaaggcatc gattcgagcg
     2461 taactgatat atccgtgggt gtgtacagca cctgtgctat cgcccaaggc gcggcgtggt
     2521 gttggggaaa gaacgatgag gggcaactcg gcaatggctc gacggcgagc agttctgttc
     2581 cgactcgggt aaacggactc gaaaccggcg tcgaggcgat cgtggcaggc tattttcaca
     2641 cctgcgccct cgttcgcgcg gcggttcgct gttggggagc aaatgacaaa ggccagctag
     2701 gcaataactc gacgaagagc agtctcgtcc cggtcgaggt agacggccta tcatccgatg
     2761 ttactgcttt gaccgctgga gcgcatcaca cgtgcgctat agcgaacggg ttagcgcgat
     2821 gttggggagc caacgatgtt ggtcaactcg gaaccgagag ggtgagcaac agtccggtgc
     2881 ccatcgaagt cagttttcag agcagccttt agttcaggcc gcgcgaccac aaaaacgagg
     2941 gaagattccg acgccgacta cggtacgagc agggacggca acgtctctgg cgcttgctcg
     3001 acggatagcg aaggagtagg agcgtttagt caaaggcagg gtgttggggt gtcagtgaaa
     3061 tcgccgaatg aggtttgggt gagaaccagc ctgggcgctg cagtcctgaa gtgttcagtg
     3121 cccggggtca cgaacacgag tgaacaagtg cgcttgaaca gccaagtcgc ctgggatcta
     3181 gttcacctct tgaacggagc acacgggctc gatgagcttc ggctggcccg tgagctcgaa
     3241 tctatactgc ccaccggtgg actcggccct ccgtcgcggg caccatcgcc gcgtcaggtg
     3301 aggctttggt tgtctgggat agcagggcgc gtcgcggatg ccctacgcgc aggccttctt
     3361 cgtgtcgaac tcgtcgcccc atctcgcgag ggggtgtggc acgatcgcgt agagcggcaa
     3421 tccgagccgg atcccgtacc ccagcgctca gcgcctccac cccgcgagcg ggtgacgacc
     3481 tggttttctc tccgcatcat cgatgaggtg ggagcccctg tagagggcgt gagtgtcaag
     3541 gtttcgaccc aagacggcga tcagacgctc accacatcca gcacaggggt cgttcgtttg
     3601 gatgacgtct ccaacagccg ctctgcatcc gctcggctgg tggaccagtc acaagtccgg
     3661 agcgtcctat cgcctcggtg gcgcgagccg cgctccagac gaaacttcgg gggttcgagc
     3721 gtcctgattc gaagtctcgc ggaagccttt cttgatccat tggagttgcc ctcggagatc
     3781 cccacaacgc tggtgttgac tccgcacttc gagtgccggg aaatccctgg cgcttatttc
     3841 gagttcggcc gaagctttgt gcgcgccgag gcgctggagc cgctcgccga catcgcagaa
     3901 gcgctccgcg gggacgagaa cttacgagcg ctgatttgtg gtcatacgga tatttctgga
     3961 agtgacgcgc tcaacaagga gttgagtgag cggcgcgcca aagccgtgca cgcgctgctc
     4021 actcacgatg cagatgcatg ggaagagctc ttctccggca cggccgacgg gagccattgg
     4081 caagagcgtt ggggcgtcaa ggaggcacaa cacatgctga acgcgctcgg ggtcacggac
     4141 gattcggggg cgccgctcgt cgagcacggt gagcgtgaca ccgcgacggt gcaggcgata
     4201 catcgctttc agcgcggcga ttaccccgat aagcccgcgg agcaagcccc gctcgcagaa
     4261 agcacgacgt tgggtgccga gggtcgtcgc gagctgttcc ttgcttacgc caaacgggtc
     4321 agccggcagc cgagcgcacg ggaccgattc acgaaggtcg gctcggcgcc gttcatgggt
     4381 tgcggcgagt tcaatccagt caaggcgacg gcgcgcgatc aggagagtcg acgcaccgta
     4441 gtattcctct tcgatcccgc ggcggagccc tcggggctac cgtgtcagct ccggcagctg
     4501 gcgccttgca agagcaactt agagcctgct tcttccaccg cgcagcgtga gcggccttat
     4561 cgttgccgca tctatcaaaa ggttgccgag cgctgcccct gccaagcagg tccagacctg
     4621 agccacgatc tcgtgctccg ctttccaatg acattggcga gcgccaacac gcttccgcat
     4681 cgctacatcc tcaccagtga agatggcaca atcaaccaaa cgcgcgagct cgccagccag
     4741 gcgagggcaa acgagcagca acgcgcggag ctcaccttcg aacacctgcc cgatacgcat
     4801 cgctatcggc tgatctgcga cgagccgaca tccacgtata cgttgttcga gttggccgcg
     4861 ctgtcggagc tgcaggagcg gctcgccact gatggagagg tgcccgagaa ggacgtgccg
     4921 ctcgagctgg cgggctggtt cgctgaggcc gaggtcgatg cccccgcctc cgactcgcag
     4981 agcgccggcg accaaaccag cttgccgcca gaagacggag cctcagcgtg actcaagccg
     5041 tagtacagcc attcttgccc gaagaattcg aacgaattgc gggaactccg actatcacgt
     5101 tggggacggc cctgtatgtg attcgcgcta cggtgttcga cccagtcagg aagctagacg
     5161 ttgccgtccc cgctcactct tgggtgttgc tcgcgggggc cgggagcaat gcgcgcccct
     5221 tcgcgcgcag caacgcaagc gactcggacg gccgggccgc actgatggcc cagcagagct
     5281 tgccagatgg cgagtactcg ctcgctctgg tgccttttcc gagtatcatc cgcgagggtt
     5341 acctgcggga ccgagaggct tggatcgatc tggaccgtgg ggactgggcg ttgccgccct
     5401 ctgccgtagc gaacctcgaa cgccgtcgcc tggtccgcat tccggcgtgg tcgacgatgc
     5461 gcaaggccgc ttcggggggc ttcaaaatcg tgcccccggg tagctcacag ttcgcgaccc
     5521 tcggcatcct caaagcggcc gaaatccagc ctttcgggtc gcctcaagaa ccctgggcga
     5581 tgccgctcga tttcaactgg gtcaaggcgc aggtacgctt cttcttctcg aactggcagc
     5641 tgaaccaaac ggaggtggtg cctccaggat tgctcgtcga ggctgtgagt gatcgtctgc
     5701 cgcctcggtc gaatccgaag cttggtgccg gcgtcgccct aaccccgacg gatggcagca
     5761 tatatatgct tttagaaggg gacgcgaccc tctggcgaga atgccagttt caattcgtga
     5821 ccccgggctc cgcctacgtg gatgttcgac agaaggcgtc cgccgcgccc gacctgcgcc
     5881 tcacgaccga gcaacctgta cccgagccca gtgaggatag gaaccgactg ccgagcgcct
     5941 ggcattcgca cggcatgcgc gccagtttcg gagacccgag cgagacgcag ccattcaaga
     6001 agcgctccgc gcgttgggat gacctgcgga agcaaatcag caaggacccc tcggcgccgc
     6061 aggccagtgt cttcttccat ttggacgacg tcgtgttgtg cgacgaagcg ggccaaccca
     6121 tcgccgtccc gagtaagcac caacctccca cgctgttcga ccatttcttg cgggtgcacg
     6181 agcagaacga gaagaagccg taccaatcca aggtggactt cgtgggcggc ggggtgatcc
     6241 ctgctgagcg agcttatccc gtgggcgaga aactcacgac gagggacaag ctactctgga
     6301 actcaactcg tttgatccac ttcgaaggcg ccttctacga cctgcgagac gaccgagtaa
     6361 cggggcgcgc tggcaggacc acggcggttg gtgcgcgttc agcacgtcgg gggcagcatc
     6421 cgcatgcggt gctcagcaag gggaatccgt acaccaacta tcatgggcct acagagctac
     6481 acgcaatcga tgttccgggc gtgacggacc cttcgaccgg tacgccgctg cagcatgtgt
     6541 tgatttacca gtcagtaatt ttcgagcgta ccccggactt tcccgagtca gtcctcccgg
     6601 aggtctatcg gaatctatac gcagccggtg agcggtggag cccgggtcat cccggcgtgc
     6661 atggtaagat ccaacagaaa tcctacgccc tggtctcaca ggagcctgca cggcgagctg
     6721 agcggaccac ccgtatccgg acgttcttgg cggagacctc ctcgggcgcc tatatcacca
     6781 ttgttctaaa gaactccgat caggctcggt ccagctacgg cgacgcgtgg ttcctgtacg
     6841 gttcgcggat cacctactat agcggggccg tgcggaccga tactgctgga atccattcga
     6901 tgttggacaa gggggacagc gtcgagcttc cctggcacac gctcgcgcac gaattcggac
     6961 acgctcttgg tctgccagat gaatactgtg agccgctgca ggtcgaaggg cttcccccca
     7021 aagtgacact caccgaacca gtgctgcctt cattcaatca gtcggatcgc ggctacggcg
     7081 cgtatcggcc cttcaattcg gaccaggccg ccatgatgaa ctcgaaccga ttgccgcggc
     7141 tgcgccattt ctggcaccac gtcaaaatcc tcaacgagca tcccccgttc gctcagctgc
     7201 ccggcattcc ctattcgctc gtgcacacga agtttggtga ttcgcagctc acctatcggg
     7261 tgcctccacg cgatgatcac tcgccctatg cgcccatctt ctcggacgcg ccaataccgg
     7321 gggggcacgg gcactgcgct atgtgggctc tgggtgacga cgaaggatcg gttcgaagtc
     7381 tttttgggct ggagaatgcc gcgctcatgc cagatcgtat gcagggtttg ctgattgtcc
     7441 gagttaggtt cagattcatc tttgctaagg gaatctccct ggagagagca tgggaggacc
     7501 tccgaaactt tcatctcaaa ttatgtgatt cgaagcagat tcctcaggtg aggttcctac
     7561 tagggggcgg tgaacccttt ccgcgcatcg cggtcgtgat acaaccgcag tacgagatcg
     7621 gccccacact ggatccattg gacccggccg atatcgtagt ctgtgttcat tcgagctcga
     7681 gcccacctcc cctgcagctc gatcggctgc atcactacga tccgcgcctc gcggtcaatc
     7741 cattcttgaa agacaatcct gactccccct tggatattct agagtcggat ctgctgctcg
     7801 agcctctcct caggcatgcg ttgggcgcca ggacatggct ctcgggttcc gggggaacac
     7861 gtaaggtcaa tctggacgcc ctcgcccccg cagacgtgac gtcactcgca cgactggtcg
     7921 agcaaaaact aggagagccg agcggccgcc gctacatcgg cgtgtactag atggcaaagc
     7981 tccgtggggt tggccagctg cgatactggc ccgatagcac acttaaatgg gccctaggcc
     8041 taatctgttg gctcatcacc gccacggctt cgtgccaagc gccccgccag aagactgacg
     8101 tgaagaacac tgtaaagaag gaggctcgcg ctgtgaagac gcctgatttt caagggaccc
     8161 tggctggcag cttctcctat ccctatgcgt cgagtttgcg gaactcgcag gtcttcgtca
     8221 aacgccgagc tctagcctca ctctcgcgga cacctctgaa atgggtcaga ggtggcatgg
     8281 acccgaacga actcggaggg ccggtgacgc tggtcgtcag ccccaacggc caacacgcgc
     8341 tcgctgcatc gggggtctac acattcaatc tcacatcgcg gggcatcatc ggcatcaggg
     8401 acatccctga tcgtgagcta cctcacgatc tcgttgcact cgatagctcc aaatacactc
     8461 agggcagtgc ttcgacgtgg gacgggcgtc ccttctacga acccaatcgg cagggatgcg
     8521 cacttgcccg gcgtatcgag gaagacaagt atgccgcagt tatcattcag gggcctatct
     8581 gggataggaa cacagagcgt tggaccgaga cttacgtgac tttagagggg ggcttgagac
     8641 acgggaaccc taatgacgtg gactggagac tttacgtgga ttgcaaaacc gccgtcgccg
     8701 cgattggtgg agaccgcgcg accgtgctcg cgctggataa tcacctgctc caggtgtacg
     8761 cgcccgaagg caacccccga ggcgaagctc ccatcctcgt cgctgaacgc aacattggag
     8821 tcgtcgccac ccacgtcagt atcgtgggct cgaacatcct ggtgattgct ccagggcacc
     8881 ccgaggaagg ctctggtagt cggctactcg cgttcaccgc cgagacacgg cccatctact
     8941 ccctgagtat gcccttcgag gtcctgcagc ccgcgattgc gggcagcggc acgcgcgtct
     9001 acctcgcagg tcgcggcctg gccgcgctgg acggaggcgg agtcagctgg ctctacgtat
     9061 cgcaggagcc aatctacgcc tcgtccttcg aagacggcag cctcgccgtc gcgcacggaa
     9121 agcggctcga gttcgtaaac acctcaggta ccgtagagca agcattcgac accgaggagc
     9181 cgctcctcgc tcccccagcg atcgcggcgg atggtaccgt gtgggccatc tcgggacgcg
     9241 cggtgtatat cgcgaggtag caatttggcg agtgcgtggc cggtgacgac gcaaggctat
     9301 tcaagtgggc gggccagcat cgagccctgc gcccctccag tgtgctcgcg cctgacgcca
     9361 taaaccctct gagtatgaag acctcgcgca gggcgctgag gttttggcta gccatcgcaa
     9421 ggcagtccta tctcgagaca caattttcgt gtgcggtgtg gctggacgac ggcgcggtgc
     9481 cggttacatt gattctccag aagcagccta caagaagcga ctgggtcgat gcgcacacgg
     9541 attattcgat gcatcaatta cctgcaggac accgggcgac gaagctttat cccagagggg
     9601 ccttgattag agcccgctgg cagcagctgg ccttgcccgc tgggctgatc gccgtcgatc
     9661 tgctgagtaa ggcctgggcc gtggcctggt tgaaagccga tcagcctgtg gacgtggatg
     9721 cggggcttca atgggtgctg cgcgtgaatc gagaggggtt gggcagttgg tcgcgcgcgc
     9781 tcgccttcgc ggcggacgcg cgagtagcgg gcgcggtgtt gtatgcgggg ttcgccctcg
     9841 cgttcttggc gtttcagcgc gggcactgga cgaaatggca gaaggtcgcg atcttggcgt
     9901 tgatcttcgt cacgtcacct ttcctgggta tcaccctgaa tttggtgatt cccagtctgt
     9961 cgaccccgtt gctgacgcgc ttgtgtggcg ccgcgttgtt cggtgtactc tggtggttga
    10021 gtaagcccgg gctttggcgc tgggcaacgg cattcatggt ctccgcgggt ctgggcaact
    10081 cgctcgggtt gttggtccac ggcgagggta tcgtcgactt catctattcg cgctggatga
    10141 cttcgctact gaacatgggt gtcttcaata tcgctgatct ctatttcaat ttagcggcac
    10201 tctgcctcac cgcctggcta ggacggtggg cggctcgcaa tgcagctaac gccactacac
    10261 gcctgctgcg tccagctggc gcgtagtggg ataggaaccg ccgcctcggc cctgaagcaa
    10321 cccgatcatt acttggccgg ccgacgcgtg accgaccacg aatatcgtct taccggactg
    10381 tccataacgc ccgagaatct cgtcgcgcgc agtcatcacc atcgataaac cagcttcgta
    10441 ggtgtcgagt ttccaatatc gatcgagctc cgacgatcgt aaaccgaacg tcgaactcac
    10501 gagctccgct gggtccgctt cgtcggactc acgatcacgg cgtcgcgttt cgcccccatt
    10561 ccgctcagga aactcgtgag ctccgcgatt cgtgagagcc cgaactcggt cagagtctcg
    10621 ctgttctcgt aggtgatctc gctcgggtcc agcgtattcg cgaccacctc tgcgcggcga
    10681 atgtaataga gcgtgacccc gctggcgcgc ctgccgccct gaggcgcctg aagcgctcgt
    10741 gccgcctctc gccgggctcc ccgctaagcc actggagctg ccgccactcg cgaactcacc
    10801 gcgcttctat ccggtgctgc cggcagtttt ccaagcagac tggcgctcag acctccttgg
    10861 ttcggcgcgc cgctccgggt tccgggcggc gctgtgccag cagatgtgcc accaggactt
    10921 gctccgccgg agctgctcaa cgctcccccc tgagaagtcg acgggctcga gcgcgacgga
    10981 acaacccagg ctcgcaactg cgcgagggct tcgttcgttg cgccaagacc gttcactcat
    11041 cacgagctac cgtttcgtcg cgtcgcctga gttgctgacc tgatgttcgt cgacgaggca
    11101 gctaacgcct tcagcagagc agaaagccgt gcagcaattg cgactccgcc cgctcgtggc
    11161 tctcgagttg ttccaccaag ctgcgcgtac ccgcagataa ctcgagccag cgctcggtgg
    11221 tttgcgccat ttctcgcaag ttgccgagct ccttcaggaa gacggcgtgt tgagacttga
    11281 gctcgctgat ttgggcagcc agcgcggggc gttcggcttc cacggtgccg aagtagccct
    11341 cggattcctc ggcagcgaag tgcgccacca gatcttcatg caagccttcc aacaagcccg
    11401 agggctcgaa ctccggcgct agcgtcggcg ggcgctgctc gagcagagcg cacatctgcc
    11461 gcaagcgacc tatcgtttgc cgcagatttt tgtggtcact caaaatcgca gtcacacgac
    11521 caaaaagctc gggcggctga gtcatcatct gctcggtcct tgcttgaggc tcggaggatc
    11581 agaccacttg catctcgcgg gccatcccca gcaggggctc taatccaggc cttggcgcgg
    11641 taaggtgagc gacgttgtgc aagacgtgca cttccggttg ttggagcgcg cccatcttgc
    11701 gcgttcggcg tggataccgc acctcgcggg agaacggagt cagacgtact ggcggaatgc
    11761 accgccacgc cccaaaacca caatgtagtc attacctctc gccgtagtgt cattgcgcag
    11821 ctccgcggta gtcaattgcg ttgtgcgcac gatcggcgcc agaggtgccc tgatccattc
    11881 ccgcggaatc cgcagtccta agtcgagcgt ggcgccctcc gacagggtcg caatcgaggc
    11941 ggctgcaccg ccagggaagg cagacgcgag cgaatcgttt gcaacgcgca gctccgatga
    12001 gaacggccct caaaggcccg gcaaagtgcc cgtcgcgaac tcgaactcgc cctgattgcc
    12061 gaagtaatcg atctctatcc cgaacgcctg cgcgatcgat accaggagcc gattgttggg
    12121 aacggtttgg tactcgccac tgttcggctc gcacccggtg ccgagtgggc aatcggtgcg
    12181 aaactgaatg cgccgcccca ttcggatctt gccgttgacg ccgcccgcca agacggtcgg
    12241 gacatttcgt gtactgtgcg ccgcgggatt gcccatgtcg ctcgacgcat aaatcaaggt
    12301 gctgtcgaga atcccggact catccaggcg ctgcattagt ctggcgattt tgccgtaaac
    12361 atagtgattg aacttggcta gccgtcgcca cgtttctgga tcttggttgt cggttgggcg
    12421 tccactattg ccgagcgcgg acgccgcata agtatgtgca actccggcgt ggttatccgc
    12481 cggtagcccg agcggattac ccgcataaga caggtcgttg agggccaagg tcgcgaagcg
    12541 cgtgatgtcg cagcccagcg cgagccccaa caaatcgatc tgccaatcgg tgatggcatc
    12601 gaagtacggc tctccaccgt tgtagcgttc gagctgcggg aacgccggtg gtcgcgcggg
    12661 caagctacag tcgccacccc ggacgaagct ctggtacttc ttctcgagcg ctcgcaacgc
    12721 ctccagatgt tgatcgagct tttgctcttc gaaggtaccc acccgcgccc gcagtcgctt
    12781 cacgtcgccg ctcacgaagt cgatcaagct gagccccaac ttgcgctggt gttcggccgc
    12841 ggcctgcgct gcgggatctt tgcctaccac cgctcgcttg aacagaaagt cgaaggccaa
    12901 ggcaggatcg atgatcttcg tgagtggcac tccgcccgcg ccaaaagata gcgtctctcg
    12961 agcgctgagc tctttgttgc ccacggcgag cgcgacactc gtcaccagcg tctcggctcc
    13021 gagcccattt tcgacggcaa ggaattgatc gatcgagctg ttccacggta accctccggg
    13081 gccgcctacg atccggctgc cggtcaagat cgtacccgcg gagttgtggc cgttggtgtc
    13141 cgacaacaag tcgatgcctt ccacaaccac gatctggtgc ttgaagctcc gtccgtacgt
    13201 tgccggatca tcgaagggtt gcagcgggct gtcggggtag tcgagggtga actcagtctc
    13261 tgtttctccc tcgcgacgag tgaacaactc cgccgccacc ccgtgcgggt gatatacacc
    13321 cacgaagcgt tgcggaagcg ccaacccctc ggactgcgcg taggcgttct cgaccactcg
    13381 caggaatggt aacgccgcaa ttcccgcccc gatggcgcgc agaaacgctc gtcgcttgac
    13441 gttcatggcc cccctcgcac ggcgaaagat gggctcttca cataggcaat cagggtctcc
    13501 acgatgttac cgcgcgcggc ctctggaatc ttccgccaag cgtccacgaa ctcgcgttct
    13561 cctggcgtag cggctgaatc acccgtggcc gaggccgccc ggaacatgaa cctcgcaaaa
    13621 cactcccgca cgatctcgct gtccgaaagc gcagccgcga gcgcattact atccgcatag
    13681 ggtccatcga actccgcgtc catggcgacg cgcaccgcgc tgtcgatctg ctttccgttc
    13741 tctcgctccc gatagcgccc catcccgtcg aattgctcga acgagaaccc gaacggatcc
    13801 acgatatcgt ggcacccggc acaaatcgaa tcccgagggt ggacgctgaa tcgctcgcgc
    13861 gtggtcagac tcggatctgg cgcgggaggc acgacctgta gattgaacga agcgggcgag
    13921 tccagcggat aacacaccac ccgtcgcgtc accgcgactc cgcggaatat cgggtggctc
    13981 tcgtgggcgt tcgcgtaagt cgccaggaat gcgccttgat tgaggatgcc gatcctgccc
    14041 gtcagccgtg aagaacccgg gagcggccct gccccggcaa cctcgtaaag tgagagcggg
    14101 cccgaattta ccgtccaatt cgcccccagg agctccccga ccgtgccggt tgattcaaag
    14161 gccaccgccc gcacgaagtc ctggctttcg gccacgatct tcggcttctc ctccgcaaac
    14221 tcggggtaca ccagggaatc cttggagctc tcggcgatgc ggtcgatccc gagccactcg
    14281 cgcacgagcc gcacgacggt atcgcgagcc gcgggattgg cctcgaacag cctgcgcgct
    14341 tgggcctcgc gctcgtccgg ttcgaacagc gcacccgagc tggccttttg cagcaactcc
    14401 gcatcgggcg gtgccgacgt gaggaagtaa gataacgaag cagctaactc gtgagccgtc
    14461 aggcgaaccg taccatcgct cgccggctgg ccctcaccca gctccgttag atacaagaag
    14521 cccgcagatt gcagcaggcc gcgcgtgacg tgggcaatgc catcttggaa actcgccgct
    14581 tcggcgccga ccgaatacag ccccatcaac gcctcgattt cctcgtcgtc gagcggccgg
    14641 cgatacacct tggccccgaa ggtaacgatg aagcgcttgg cacaattcgc agcgtccgcg
    14701 gctgtctcac acggagcgag gcgggcaaaa gtcccattct gtttggcctc atccaccaaa
    14761 gtatcagcgg catcggccaa acgttccacg agcaccgcat cgatgcgctg cgtgtcgttg
    14821 acggtaaaac cgtcttgccg caaatctggc gggaaatcag ggccacccgc gaggctccgt
    14881 ttcgtggaga gcagcacctg cacggaagta tcgtattcta cgttcgcgag tcgacggatg
    14941 cgcgttggca gatgcgcaag tgcggaatcc gcatcgctgg atccagcgcc tccagacgag
    15001 ttaccgcctc cgaagtccgt accggtcgcg ccggccgagg ccgttgacgt cccacccgtg
    15061 ggcgaactgc tcccgttgga gaaactgcta gccggcgcgg aagcggatcg ctcggcagaa
    15121 gacgaacaag cacccagcac cgcggcgcca cacaccaaag accagattag cgcgctacct
    15181 cggctcatct tgagagaaga aaagaggccg agacaacctg cctcggccta ctcctgctgt
    15241 ggagccagcc catgaggacg ccggctcgaa cctggcgctc agacttcact cacgcgaacg
    15301 gatcccaggc caggctaccg ctcaacatcg gcacatccca gtcaatccac ttggccttgt
    15361 cgaacttgcc cagcttgctc gtcgatgagc tgacatggaa atcatcgaca ttggtatccg
    15421 ccatcccacc cttgaggaag cgggtgacga aggcgttcag gtaattttgc tgagccgatg
    15481 ggaactggca atggctgtgg ttggcggcaa ccgatacacc gcagcgatcc ttgatgccca
    15541 agccctggaa cagcaattgg gccgccgcac cgccgccgta gcacgcgacc gggcccagcc
    15601 agtcgatgtc gttgtcgatc accagcacgg cgcgcggcgc gcaaagcgca atgaccgtat
    15661 gctgatcagc catcagctta tccactgaat tggcgtagga cttgaagtcc gaaccttgcc
    15721 agttcgcctc gccgacgatc tcggtggctt cctggatgtt ctgaccggcc ttcttctccg
    15781 cggtggagac gcgccacagg gcggggccgc ccgatccgcc ttcctgcgga atcgtgagtg
    15841 ccacgcgctc gtcgaaagcc cccatcgcca aagcgccctt gccattgcgc gaacatccag
    15901 tcaccgcgat gcgcctcaca tcgatgcctg cctgcggaca cttctcgagt gcatcgatga
    15961 tccggctcaa gccccatgcc caaccgatga gcgagccgct cttggccgac atgctgccat
    16021 acagcgtatt gaccaacccg ccagaaccaa ccgtcttcga ctcttgagaa atggtgttgt
    16081 tgggcatgtc gatcttagcg acgcccgaga gcgacggcag cgacgaccca ccacaagtaa
    16141 tcaggcaagg caccggcttg gcttcggtcc cgccaccgct gatcgtgacc gagaacgagc
    16201 ctgtcttgct cccgacggtc atgttgatag tcagcttgcc gccggagaac gtcgcctcga
    16261 gcttgtctgg aggtggcatc ttcgggccgt agatacaagc ctgggcgaga gcgctcaact
    16321 ccgccctcaa gcacgcccaa tcctccttct tgctaatgcg cgtgccgttg atgaacttga
    16381 acggatccgg cagcatcgga ttggcagtcc ccatagagat attggggatc atacaggcct
    16441 tgccgcgatt ctcgacccaa gccgaaccgg gcgagccgcc gccagtgctg ccacccgcgg
    16501 gtgagccagc cgcggcaccg cctgcagaag taccgccagt cacgggcgag ccaccggaag
    16561 taccgcccgc cggagaagca ccacccgaag gcgttccgcc cgcagccgga gcaccgcccg
    16621 cagatgcacc gccctgggcc gtagtaccgc ccgaagagct agtgccaccg ctcccgatca
    16681 caccgcccgt cgcggatgcg ccgccggtca ccgcgctgcc gcccgcaccc ggagccacgc
    16741 cgcccgatcc aaccggcgtg gctccgcccg tagctgcgct tccgccgtta cccggagctc
    16801 cgcccgtacc cgtgccaata gggtcagtgc tcgagcaagc atccacgaag ccagcgacga
    16861 cgaccgcgcc aatcctcaga aagccgcgcc tcgacccagc ccgtacgtcc agcccttcgt
    16921 ccgtgacgac atcgacagcc cctgaactcg tacctgtttg atccggcatg gctcctcgtc
    16981 agtgacacac gacgccaaag caatcgtgca actgttgtcc taggtcttgt gtcctcggtc
    17041 ttgtgtcctc ggtcttgtgt cctcggtctt ggtggtgtgt ccgccacgac caagaaccgg
    17101 ctcatctctc gatatatggg cgacctttca gtgctgccgc cgtggcggtc ataaaaatcc
    17161 aacctcgatt ttcaggccgt cgcgcgggct cgtaaatatc tcgaattcga gaatcgatat
    17221 ttcggagccc agcgcggcga acctgaatca acagccttgg ctcgtgcgaa gcccagttca
    17281 cgagatcagc agaatggccg ccccgcggta aagagcgcga gaatcgcgag gctctctcga
    17341 gcgcagcctc cgtcactcga tgtcccccaa tcaccgcgca cgcggcaccc agtcaactgt
    17401 tcaacacttc tgacaacgct cgggcggccg aaggtgaacc actcgggggg cagtccaaag
    17461 caggcccaat tgggatcatc gagtatgggt tccagggaag tttctggccg cagtatcaac
    17521 aaacgacgaa agccgtcact cgaggccaga tgggggagct gaaacgctgc acggtctact
    17581 ccggactttg tccttgcaca gaaagccggc gtacccatca gtggcgctcc ccccccccgg
    17641 cggacaggtc caagttttct cgacgtctga gcttccgcgg cgcgtcccaa tccggggtgt
    17701 cgctatgacc gcacgaaatc gagcaagtct cgattcagct gcgccttgtg ggtgtcggcc
    17761 aggccgtgag gcgctccttt gtaaatcttg tattggccac ccttgacgag cttcgccgag
    17821 agctccgccg acgccgcgat cggaacgatc tggtcgtc
//
LOCUS       DASTKU010000552         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1893324, whole genome shotgun sequence.
ACCESSION   DASTKU010000552 DASTKU010000000
VERSION     DASTKU010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893324"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..1427)
                     /locus_tag="VFQ61_19155"
     CDS             complement(342..1427)
                     /locus_tag="VFQ61_19155"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9956628.1"
                     /translation="MKTSPRRKKGAIATIATVASRARVAPMTVSRVINGGYASPEVRA
                     RVERAIKELGYAPSSTARSLRYGRRGCIGVAVESVHGAWFMGILGGIEEELTRRHVGL
                     MLTSLKPKGRYDSSTVAAWIEGKRVDGIISARFTRQEQPLLDAAIKAGIPVTFICPDH
                     SVDVGYTVRCRNYDAGKQMGAHLLELGHRRLGFVGGPKESIDTMDRLRGLEEAVSAVP
                     GATLRSEDVTFQSSYRPEAGAAAGVSFLKRAPTKRPTAMVFGNDSMAIAFICEMLRAG
                     LRVPADVSVGGFDGVEEGERCFPTLTTVAQPMHRLGVAACSALLERIENPAQDQGISA
                     EFPMQLVRRESTGPLPGATPRRSKKSG"
     gene            complement(1624..1986)
                     /locus_tag="VFQ61_19160"
     CDS             complement(1624..1986)
                     /locus_tag="VFQ61_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956629.1"
                     /translation="MFRARQESGEDESRYEIKEARRVFEARTSERFSRIEFALRVLDL
                     LRPNMNVTLYESLSRIQVRRGRDWSVGAEASWALVAIPRTADRHSIAFALAELTGRAD
                     QAFVVDLVARAHQLSLAS"
BASE COUNT          387 a          837 c          536 g          344 t
ORIGIN      
        1 accactcaac ccgcccgctg taccccgcag cctgcccact cgaaaggagc gctcctcgcc
       61 acacgcgatc cccgcccgcc gtcgcgaacg cgacaacccc acccgggact cgatcgtgtc
      121 caacccaagc accttcttcg catctccacg tacccgcacg ctcctgcttc gcgccccaac
      181 cccgcgcccc aacccacgcc tacgtcaccc gaactgcaga ccgaaccaga aattaggacc
      241 cccccaccaa ccacgcggct tctcgcaccc acctgatatc gttaccacac cccgcaggtg
      301 ctcggcaaga ccacacttca cccgcgggtt acatggcgaa atcagccact ctttttcgag
      361 cgacggggcg tggccccagg cagcggcccc gtgctctctc ggcgcaccaa ctgcatcggg
      421 aactccgcag aaataccctg atcttgcgct ggattctcga tgcgctccaa cagcgctgaa
      481 catgcggcca cacccaagcg atgcatgggt tgcgcgacag tcgtcagagt tggaaagcaa
      541 cgctcaccct cttcgacgcc gtcgaagcca cccaccgaaa catctgcggg cacgcgcagc
      601 ccggcacgca acatctcgca gatgaacgcg atggccatgc tgtcgttacc aaacaccatg
      661 gccgtcggtc gcttcgtcgg cgcgcgtttc aagaacgaca cgcccgcagc cgcgcccgct
      721 tcgggccgat acgaactctg aaatgtcacg tcttcggaac gcagcgtcgc gccaggcacc
      781 gccgacaccg cctcttccag gccgcgaaga cgatccatcg tatcgatcga ctccttcggg
      841 ccaccgacga agcccaagcg tcgatgcccg agctcgagca ggtgcgctcc catctgctta
      901 ccagcgtcgt aatttcggca tcgaaccgtg tacccgacat cgaccgagtg atccgggcag
      961 atgaacgtca ccgggatgcc cgccttgatg gccgcgtcga gcagcggctg ctcctgccgc
     1021 gtgaaccttg cggagatgat cccgtcgacg cgcttgcctt cgatccaggc cgctaccgta
     1081 ctcgagtcgt agcgcccctt cggcttcagg ctcgtcaaca tcagccccac gtgccggcgc
     1141 gtcagctcct cttcgatgcc gccgaggatt cccatgaacc aagcgccgtg caccgattcg
     1201 accgccacgc cgatgcatcc ccggcgccca tagcgcaagc tgcgcgctgt cgacgaaggc
     1261 gcatagccga gctccttgat cgcccgctcc acgcgcgctc tcacttcggg gctcgcgtag
     1321 ccgccattga tcacacgcga caccgtcatg ggcgcgaccc tggctcggct agctaccgta
     1381 gcaatggtcg cgatggcgcc cttcttgcgc cgcgggctgg tcttcatcct cccgaacata
     1441 ccctaggacc catcccccag ggtaactacg cgccccgact aggcagccgg cgcgatattc
     1501 tcgggagatc ccgccgactt tagccggatg atatcgctac caggtccact gtcgggcgac
     1561 ggactccctg gcccgagctc gatcccgctc accgcttgcc gcgcatgccg tcgggacccg
     1621 acttcaagac gcgaggctca gctgatgggc acgggccacc aaatcgacca cgaacgcctg
     1681 gtcggccctg cccgtgagct cggcgagagc aaaagcaata ctgtgacgat ccgccgtccg
     1741 cggtattgca accagcgccc aactcgcttc cgcgcccacc gaccaatcgc gcccccggcg
     1801 aacctgaata cgactgagac tctcgtacaa agtcacgttc atgttcggcc gcaacagatc
     1861 cagcacccgc aacgcgaact cgatccgcga gaacctctcc gaagtccgcg cctcgaaaac
     1921 ccgccgcgcc tctttgatct cgtaccggct ctcgtcttcg ccagattcct gtctcgcgcg
     1981 gaacatcccg tcaacctagc acccttcctc ccccccgctc gctctgcgct ccgcgccccc
     2041 agctcgctct gcgctccgcg cccccagctc gctctgcgct ccgcgccccc agctcgctct
     2101 gcgc
//
LOCUS       DASTKU010000553         4589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_107485, whole genome shotgun sequence.
ACCESSION   DASTKU010000553 DASTKU010000000
VERSION     DASTKU010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107485"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..280
                     /locus_tag="VFQ61_19165"
     CDS             32..280
                     /locus_tag="VFQ61_19165"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HET9956630.1"
                     /translation="MVSPNQIQPNAPVVCSKNGQFAVVDHIEGNAIKLKKDQSGQHHY
                     IPLDWVTSVDDKVHVDRPGDQAMREWLTSPPSTSQRSS"
     gene            complement(587..1126)
                     /locus_tag="VFQ61_19170"
     CDS             complement(587..1126)
                     /locus_tag="VFQ61_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HET9956631.1"
                     /translation="MSQVVLGILDTASRADTVVDGLQRAGFNLSEVSVLFPDQSGTRD
                     FAYEKGTKAPEGAVAGVGTGGVIGGTIGLLAGIGALAIPGLGPFIAAGPIMAALSGAA
                     AGAAVGGVTGALIGFGIPEIQAKHYEGKLRSGAVLIAVHTETSEARKSAETILKNAGA
                     HDVTATSEASVPRDQRAHA"
     gene            complement(1352..1525)
                     /locus_tag="VFQ61_19175"
     CDS             complement(1352..1525)
                     /locus_tag="VFQ61_19175"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET9956632.1"
                     /translation="MADNSLSVLAKNVKIITAGGKVTLRGPVKTQDERSAIQAAAQKV
                     AGVTQVDNQLEVK"
     gene            1554..2078
                     /locus_tag="VFQ61_19180"
     CDS             1554..2078
                     /locus_tag="VFQ61_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956633.1"
                     /translation="MRSEALLPWSPGVTLLSLRSRSLRPVLSGARVVPAVVEFPCALF
                     VRGDAVLVPELPVVPELVDVPVVVPVVELPVDALPELPVPVVVDVLERVPDVLLPLVP
                     VALFEELVSPLAVSVIEVPIVRLSALVVPVVPRVFSLLVGSVELGGTSIPATLLWAGV
                     VVVSSAADCAGLDS"
     gene            2847..4238
                     /locus_tag="VFQ61_19185"
     CDS             2847..4238
                     /locus_tag="VFQ61_19185"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9956634.1"
                     /translation="MSCVLLLSCSHAPEPTSTGGAGGARGPSNGIEGVSGSIGRTMTS
                     GGATQGSSGMSALGVGYDGGTNAGGAAGPTHGGSGSLGVGNADGNASGSSTPANTGGT
                     AGVPNSMNPNAMNLGGTRSGCSNPLPLDSGGLGSGGLAAPSEPTQRYSFFAFGDAHAG
                     QNDRANSSLKTAVAQMKRLDASAVLAISNGDLVEDSSAPDWAAHDAAISLMFDPKTTL
                     SAPGMPRYLAAMDNHDQGFPVPRSDWLAQWNQHLPGQQLLGHNGKDGIYYAVVYADTL
                     FIVLDSLHPSAAQTKWLASLFESPETARIPVKLAFFHEPIYACSSEHPAFAAGLAWAD
                     LFETQGVKLVFVSHLHFYDRTCPLWRGACRADGKGVVYQQLGPLGANNFRTVDLAQAK
                     VSGKDYAGNARTDNFACSGANSVFLKSRTNVNDFCHVRVEGCRVTGNCYEVGEQTSEP
                     FDTWQVDACTPTP"
     gene            complement(4260..>4589)
                     /locus_tag="VFQ61_19190"
     CDS             complement(4260..>4589)
                     /locus_tag="VFQ61_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9956635.1"
                     /translation="KTWLAAPEFVHLVFMHIPPTEPIGVRNGAFASRAEAAQLLAQLG
                     WASVAATFYGHIHSYYAFENVGIPAFISGGGGAVPERFDGIGRHFLVVSVDPHSRAVG
                     TRIVRVD"
BASE COUNT          838 a         1443 c         1409 g          899 t
ORIGIN      
        1 gggtggcacg cgaggtgctt agtcacccga catggtatca ccgaatcaga ttcaaccgaa
       61 cgctcccgtc gtgtgctcca agaatggcca gttcgcggtt gtggatcaca tcgagggcaa
      121 tgccatcaag ctcaagaagg accaatccgg tcaacatcac tacattcctc tggattgggt
      181 gaccagcgtg gacgacaagg ttcacgtcga tcgccccgga gaccaagcca tgcgggagtg
      241 gttgacgagc ccgcccagca cctcacaacg cagctcctga ccgagccgcg tagcgacatc
      301 atctcgagtc tctcctgagc cgcgttatct gccctgctct ggcacgtcga aagtgcccag
      361 cagagcggat aaggcggctt tcttttgtgt cggcggcgac gctttccaac gcttctaatc
      421 gccgtcgcag cgacagctag cccgcacaaa aaaagatgcc ccgcctcgcg aacccttgtt
      481 ttcgcgggcg gggcgactgc tcaggcgggc ttcagcctcc atccgaagcc aacctcagca
      541 gaatggctcg tgacgctgag ctattcgttg ttgcgactag ctcggatcaa gcatgcgcgc
      601 gctgatcgcg gggaaccgag gcttcggacg tagccgtcac gtcgtgtgcg cccgcgttct
      661 tgaggatcgt ctcagcgctc ttgcgcgctt cgctggtttc ggtgtggacc gcgatcaaaa
      721 ccgctccact gcgcagcttg ccctcgtagt gcttggcttg gatctcggga ataccaaatc
      781 cgatcagagc gccggtgacg ccgccgaccg ccgcacccgc cgccgcgccg ctcagagcag
      841 ccataatcgg ccccgccgcg atgaacggcc ccaggcctgg aattgcgagt gctccaatgc
      901 cagcaagcaa gccgatggta ccaccgatca cgccgccggt tcctactccc gcgaccgctc
      961 cttctggagc cttggtgccc ttttcgtagg cgaagtctcg tgttccgctc tggtccggga
     1021 acagcaccga gacttcggac aggttgaaac cagcccgctg caggccgtcc actacggtgt
     1081 cagcgcgcga agccgtatcc aaaattccca aaacaacttg gctcatggtt ctttctcact
     1141 agcgaaccta tcccgcgagg ccgggttggc actgcccatg gttcttccga gctcggtccg
     1201 cgagtgcctc gccctattgc gaggattcgc ggagacgtgg ctccgttggg caagtcaccg
     1261 gggcggccta gtgggataga ttcaggttca agttgaagcc taggattcgc ccgtttacgg
     1321 gagcgccggg gacgctccgt tgaactgtct ctcacttcac ttcgagctga ttgtcgacct
     1381 gcgtgacgcc cgcgaccttc tgggccgccg cttggatcgc agagcgctcg tcttgcgtct
     1441 tcaccggtcc acgcaaagtg actttgccgc ccgcggtaat gatcttcaca ttcttggcga
     1501 ggacggacag tgagttgtcc gccatgacgg ctttgcgaat agcttgagtg atcttgaggt
     1561 ccgaggcatt gttgccttgg tcgccgggcg tgacgttgtt gtcgttgcga tcacgctcgt
     1621 tgcgtccggt gttgtccgga gcacgagtcg tgccggcggt cgtagagttc ccctgtgcgt
     1681 tattcgttcg tggggacgcc gtactcgttc ccgagctccc ggtggttccc gagttggtcg
     1741 atgtgccggt cgtagtccca gtggtggaac tacccgtgga cgcactgccg gagttgccgg
     1801 tgcccgtggt ggtcgatgtg ctggagcgcg tacccgacgt attgttgccg ctcgtacccg
     1861 tagcgctatt cgaagagctc gtgtcaccgc tcgcggtgtc ggtcatcgaa gtgccaatcg
     1921 tgcggctgtc cgcgctggtt gtgccagtgg tcccgagggt gttttcgttg ctggtgggca
     1981 gcgtagagtt gggcgggaca tccatacccg ccacactgct ctgggccggg gtggtagtgg
     2041 tctcgtccgc ggcggattgc gcaggcttgg actcgtgaga gcaagctgcc aaaagtgcca
     2101 ccgcaccggt caggatcacg aatctgtttt tcattgaggt tccgttcatc gaaggctttg
     2161 ttgtggcgat atcgagtgtg gccaggagcg acgcagcgtt tggccgatga gtcgcgtcgc
     2221 cagctcggcg cgcacgtgtg tcacgccctg cgcatgattg cgcgggactc gagatctcac
     2281 ggcgcacccg agcgccgtag gcgaccgcag acacgagcaa gacctaggcc agcaagaccc
     2341 aggccagcaa gcccagctca gcaagcccag ctcagcaagc ccagcccagc aagcccagcc
     2401 cagcaagcgc cctgtgggca gctggaagtc cggtgagcct cggatgagta tgcgagtcgg
     2461 cgtttgagtg aaccgcggga atctcgcctg ttcgtcttgg ccttttagtt tgcctcatca
     2521 tgcctgtgga cgaccgaatt cagaacacgg tccagacgcc acaatggggc gaacgcgcga
     2581 gcgcgaatca gttcgagtca gagcgctgtc gtaccccgcc gatgaggaat ttggcactca
     2641 ccgaggaaac tgacccgctt tggcgaattc gcgactctgc aatcggctac atgcgtcttc
     2701 attggcaaga ccacgcttca tgcgcttccc atttggcctg ctgcacgctc agcacgaatt
     2761 cgccaaacgc tcaatcgaag ctccagactc gcgcactatt cactgacgtg cgtcgcgggc
     2821 gaaccttacg atggttaccg gtgctgatga gctgcgtgct tctgctcagt tgctcacacg
     2881 cgccggagcc gacatccacc ggaggagcgg gtggcgcgcg agggccctcg aacggaatag
     2941 aaggcgtatc tggttcaatc ggacgcacga tgaccagtgg cggcgccacg cagggctcat
     3001 ccggaatgag cgcgctgggc gttggctacg acggtggaac gaacgcggga ggcgcggccg
     3061 gccccaccca cggcgggtcc ggctcattgg gagtcggcaa cgccgacggc aatgcaagcg
     3121 gaagctccac accagccaat accggcggaa ccgctggtgt gccgaactcc atgaacccga
     3181 acgccatgaa cctgggaggc actcgttctg gctgcagcaa ccctctgccg ctcgattccg
     3241 gcggactcgg ttccggcggg ctcgcggcac cttccgaacc cactcagagg tactcttttt
     3301 ttgcattcgg ggacgcgcat gccgggcaga atgatcgcgc caacagctca ctcaagaccg
     3361 ccgtcgcgca gatgaagcgg ctcgacgcga gcgcggtact cgcgatcagc aatggtgatc
     3421 tggtcgagga ctcgagtgcg ccggactggg ccgcgcatga cgccgcaatt tcactgatgt
     3481 tcgatccaaa aaccacgctg agtgccccgg gcatgccgcg ctatctcgcc gccatggaca
     3541 atcacgatca aggcttcccc gtgccgcgct ccgattggct ggctcagtgg aatcaacatc
     3601 tacctgggca gcagctgctc ggccacaacg ggaaggacgg catctactac gcggtggtgt
     3661 acgcagatac gttgttcatc gtgttggact cgctgcatcc ttcggccgcg cagaccaagt
     3721 ggctcgcgag tttgttcgag agccccgaga cggcgcgcat ccccgtcaag ctggccttct
     3781 ttcatgagcc catttacgcc tgctcttcgg agcacccggc ctttgcggcc gggctcgcgt
     3841 gggcggatct gttcgaaact cagggcgtca aactagtgtt cgtatcccat ctgcatttct
     3901 acgatcgaac ctgcccgctg tggcggggtg cctgccgggc ggatggcaag ggtgtcgttt
     3961 atcagcagct cgggcccctc ggggcgaata acttccgcac tgtcgatctc gcgcaggcca
     4021 aagtctccgg taaagactac gcgggcaatg cgcgcactga caatttcgcc tgcagtggag
     4081 ccaatagcgt gtttctgaaa tcgcggacca acgtcaacga cttctgccat gtgcgggtag
     4141 aaggctgtcg cgtcacgggc aactgctacg aggtaggcga acagacgagc gagcccttcg
     4201 atacttggca agtggacgcc tgcacgccga cgccgtagtc gacgtgcgcc ggacgtccct
     4261 caatcgacgc gcacgatgcg ggtgcccacc gcgcgtgaat gcgggtcgac actcacgacc
     4321 aaaaagtgac gtccgatgcc gtcgaagcgc tccggcactg cgccgccccc gccactgatg
     4381 aaggcgggaa tccccacgtt ttcgaatgca tagtagctgt ggatatgacc gtagaacgtc
     4441 gcggctaccg aagcccaccc cagctgagcc agcaattggg ccgcctccgc gcggctcgcg
     4501 aacgcgccat tgcgcacccc aatcggctcc gtgggaggaa tgtgcatgaa caccaggtgc
     4561 acgaactccg gcgcggcgag ccacgtctt
//
LOCUS       DASTKU010000554         6528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1223397, whole genome shotgun sequence.
ACCESSION   DASTKU010000554 DASTKU010000000
VERSION     DASTKU010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223397"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..510
                     /locus_tag="VFQ61_19195"
     CDS             172..510
                     /locus_tag="VFQ61_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956636.1"
                     /translation="MSDTHHRRRANPELPVTNIVTYVPKEGKEAELLALVKKHEPALR
                     KVGLVTSEPFRVWKAFDIRKQRQLFIEQFVWKDGNASDIAHQTPEVMAVWEPMGPILE
                     ELTICEVEPA"
     gene            549..818
                     /locus_tag="VFQ61_19200"
     CDS             549..818
                     /locus_tag="VFQ61_19200"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9956637.1"
                     /translation="MVAALAHAARRQILLTIHLRGGATAGEIASRFAHRWPTTTRHLR
                     VLESAGLIRQQRIGRTRVYSVEKDRLALVREWLAWFDSPAPGSGD"
     gene            complement(886..1767)
                     /locus_tag="VFQ61_19205"
     CDS             complement(886..1767)
                     /locus_tag="VFQ61_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956638.1"
                     /translation="MFGWLRRRDDREELIVDNRVFAIGLDGMVRTALGRHEKGELLAC
                     AAEVAKALGIGASSSPVEGNYTEDPDRARYFSLMRALQGESMHREREVAHMAEFQRLM
                     AVTSSRIFGLADRDGKLLPRGKDALSQAMETSPLTVKDLTAAAAIAAERSDDISLVGV
                     AARTKDAVCLAATRETCVLYAKVYLGAVPQAFDYTWRVDPSVCTAAKLFLRQFCALFG
                     DVLPEPEPGHAKDYFDAYQSNQVTGRCVCLAAEHERYYHWAIVADGADSRVEEFWDST
                     LWTTERFRATRPPLGSP"
     gene            complement(2144..2587)
                     /locus_tag="VFQ61_19210"
     CDS             complement(2144..2587)
                     /locus_tag="VFQ61_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956639.1"
                     /translation="MAILAFGALFAALYCGVMAMLASMSGWSRLAERYPANRRFKGPS
                     RSGVALQLEYGAYRGVCRVGSDHSELNISLPLLFRFRHPTLSIPWAEIVSCRPGEFFG
                     TRTAVLRTRAVPEVPIAIPEELAKWLESVSPLASCPGRTEGKGRT"
     gene            2886..3137
                     /locus_tag="VFQ61_19215"
     CDS             2886..3137
                     /locus_tag="VFQ61_19215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HET9956640.1"
                     /translation="MSTEKLRIEVLALPANERAELAHVLLESLHADEDPAAEEAWLVE
                     LDRRAQSVADGTATLVPWETARERIKAQLKARRGRRTNL"
     gene            3115..3423
                     /locus_tag="VFQ61_19220"
     CDS             3115..3423
                     /locus_tag="VFQ61_19220"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET9956641.1"
                     /translation="MDVVLTSEAQLELDHAAAWYDERHEGLGDEFVDEVQDALATILE
                     TPETWSRWPGAPARIPAVRRFVLPRFPYSIAYQVHIGRVVVLAVVHARKRPLYWIARL
                     "
     gene            complement(3880..4305)
                     /locus_tag="VFQ61_19225"
     CDS             complement(3880..4305)
                     /locus_tag="VFQ61_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9956642.1"
                     /translation="MSIGAPDTGERGWGARWCAVLLPAVVASVGCGSAPLQAPGVPPR
                     GQATLAVLSYNLNYGIAGDEATLDAIAAQRSDLVLLQETTPEWERALRARFGEEFAHM
                     AFRHFQRERARQRGFVARITGVTLGPARLQPGRYVELEH"
     gene            complement(4412..5770)
                     /locus_tag="VFQ61_19230"
     CDS             complement(4412..5770)
                     /locus_tag="VFQ61_19230"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ricin-type beta-trefoil lectin domain protein"
                     /protein_id="HET9956643.1"
                     /translation="MSPLESPSSRHSRMPRSGFDAARGGVALGMLALVFGCASAAQSE
                     DESIVSTQRAVAGGTFTTTPRWSVGWGRRSFGVACSASVLNEHWLLTAAHCVEPLDAE
                     EFIEVFFANSAGERTRIFDGRAVPLAHPDHTQSSYAPHDVGLLYLASSGLNLTAIGKA
                     KLYADPRRPWADSDEPNEMFLAGWGNNNDDCDREDGADTGIGLLRQGSNLHVDPNAAN
                     SHFASAGIDSVHTCNGDSGSPYLLFRGTESSADFLEFAVHSGRRFMSLPWSPLSTRRH
                     QGPLIEDNIAWIQQTLTESTSAVFEETWVSASHGSYLYRQSRITALDYGDVQGYQGRC
                     LHDAGASGVRLRNCDGSRRQRWRFELSGEVKREAGPLDRAPGCLSASSTANRTQLTVT
                     DCNGSPSQKFWYDSWGQLRPTIADLFSPSRKKCVEAPGAANDSPVQVFDCNGTAPQVW
                     LP"
     gene            complement(6213..6528)
                     /locus_tag="VFQ61_19235"
                     /pseudo
     CDS             complement(6213..6528)
                     /locus_tag="VFQ61_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611348.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT         1314 a         2015 c         1995 g         1204 t
ORIGIN      
        1 cagtgcagac gccgagcgca cggggcttgc gcgagcaccg ctgacgcgcc cagttcctca
       61 ctaaacattt gaaattgctg aataattaaa tttagcagag gagctaaatc tcgctggcca
      121 atattgagcg aaacggctaa atgttcagct cgaactctgg aggaccatct catgagcgat
      181 actcaccacc ggcgccgggc caaccccgaa ctccccgtca ccaacatcgt gacctacgtt
      241 cccaaggagg ggaaagaagc cgagctgctc gcgctcgtca agaagcacga accggcattg
      301 cgcaaagttg ggctcgttac gagtgaaccg ttccgggtct ggaaggcctt cgatattcga
      361 aagcaacgac agctcttcat cgagcaattc gtgtggaagg acggaaatgc gagtgacatc
      421 gcgcaccaga cgccggaggt gatggcggtt tgggaaccga tgggtccgat tctcgaggaa
      481 ctgaccatct gcgaggtgga gcccgcctga cgttaaaacc cgacgaaaag ctggctgcac
      541 tcgacgccgt ggttgccgca ctcgcgcacg cggcgaggcg gcagatcctg ctcacgattc
      601 acctgcgtgg aggagcgacc gcgggcgaga tcgcgagtcg attcgcccac aggtggccca
      661 cgaccacccg acacttgcgc gtgctcgaga gcgccgggct gatccggcag cagcgcattg
      721 ggcgcacgcg agtctattcc gtggagaagg atcggctagc gctcgtccgc gagtggctcg
      781 cgtggttcga ttcgccagcc cccggcagcg gagattgaat acgttagtcc ggtgacccga
      841 cggtcgacgt gttcacctac agcaagcggc gttggacgcg cgggctcacg gggaccccag
      901 aggggggcgg gtagccctga atcgctcggt ggtccacaga gtggaatccc agaactcctc
      961 aacacgactg tccgcgccat ccgcgacaat agcccagtga taatacctct cgtgttctgc
     1021 agccaaacag acacatctgc ccgtgacttg gttgctttga taagcgtcga agtagtcttt
     1081 ggcgtgaccc ggttccggtt cgggcaagac gtctccaaac agcgcgcaga actgcctcag
     1141 gaaaagctta gccgcagtac atacggacgg atctaccctc cacgtgtagt cgaacgcctg
     1201 gggaacggcg ccgaggtaaa ccttcgcgta caagacgcac gtctcccgtg tcgccgccag
     1261 acagactgcg tccttcgttc tggccgcaac gccaacgagg gagatatcgt cgctgcgctc
     1321 ggctgcaatc gcggccgctg ccgtgagatc tttaactgtc aaaggcgagg tctccatcgc
     1381 ctgagagaga gcgtcttttc ctcgaggcaa gagcttgccg tctcgatcgg ccagaccgaa
     1441 aatccgagac gaagtgaccg ccattagcct ctggaattcc gccatatggg cgacctctcg
     1501 ctcgcgatgc atgctctctc cttgtaaggc gcgcatcaga gagaagtagc gggcacgatc
     1561 gggatcctcc gtatagttgc cctcgacggg actgctcgac gcaccgattc ccagggcttt
     1621 ggccacctcg gccgcgcacg ccaggagctc acccttttca tggcggccca aagcagtacg
     1681 gaccatacca tctaacccga tcgcgaacac tcgattgtct acaatcagct cttcccggtc
     1741 atcccgccgc cgaagccacc caaacatgtc cgatgaaata gcagcacgat cacgctccgt
     1801 caattcggcc gggaatgcgc taccgcgcgg cgttccggga actcgaccag caagtccatg
     1861 actgggtcgg cttacctgag aatcccgtga cacgtcccgc ctgctcatgc agccgaaggt
     1921 ggggagcttc gaagaccgtg agggtggcgt cctgtcgacg atctacaggt attgctaact
     1981 cactagctga ctcgggaggg tctccgctcc atgtgagaaa gcgaacaccg cgcgcgtctg
     2041 tgacgctgta gtttaggaaa tgtgccttct gtggagcaca ggatcgacgt ggcagcgacg
     2101 cacagaagcc atgagtagag ctgggatggg gagagctcgc caatcatgtt ctacccttcc
     2161 cttcagtgcg ccccggacaa ctcgccagtg gagatacgga ttcgagccat tttgcgagct
     2221 cctcgggaat cgcgatgggc acttcaggga cggccctagt tctcaatacc gccgtccgag
     2281 ttccgaagaa ctccccaggg cggcacgaaa cgatctcggc ccaggggata ctcagggtgg
     2341 gatgccgaaa ccgaaagagg agtggcaacg agatgttcaa ttccgagtgg tccgagccga
     2401 ctctgcagac gccgcgatag gccccgtatt cgagctgtag cgcgactcca gagcgtgacg
     2461 gtcccttgaa cctgcggttt gcagggtaac gctcggcgag ccgcgaccag ccgctcatcg
     2521 aagcgagcat ggccatgact ccgcaataca gagcggcgaa gagcgcaccg aacgccagga
     2581 ttgccatcgc tcggaactct tgcgcagacg tctcgtaaag caactagtgc aaagagggcg
     2641 gtgtttgcca tcccctcagc acacgcgaga tcgcggcgcg cctcgaacgg cgtgggtgca
     2701 ggcaacgcgt cgtgcgcggg aaatcggcag tggacgggaa tacttctggt gtttggcgca
     2761 gaggaggcat agccgtcgtg gaagggcgac gttggcgcgg acgcgggacg ggcccggcgg
     2821 acgccctctc acctccgagc gtgcggtgct cgccagtggc gtacaatgcg ctaagctggc
     2881 gtgaaatgag caccgagaag ctccggatcg aagtgctagc tcttcccgca aacgagcggg
     2941 cggagctcgc ccatgttttg ctcgagagtt tgcatgccga cgaggacccg gctgctgaag
     3001 aggcgtggct agtcgaactg gatcggcggg cgcaaagtgt ggctgatggc accgcaacgc
     3061 tggtcccgtg ggaaactgcg cgcgaacgca tcaaagctca actgaaggcg cgacgtggac
     3121 gtcgtactaa cctctgaggc acagctcgag ctggaccacg ccgcagcttg gtatgacgaa
     3181 cgccatgaag gcctcgggga cgagttcgtt gacgaagttc aggatgcgtt ggccaccatc
     3241 ctagagactc cggagacctg gtcgcgttgg ccgggtgcac ccgccagaat tcctgccgtt
     3301 cggcgcttcg ttttaccgcg ctttccttac tccatcgcgt accaggtgca cataggtcgt
     3361 gtcgttgtgc tggcggtcgt tcatgcgagg aagcgtccct tgtactggat cgcacgctta
     3421 taactcacgc cgaaggcaca actgtcgccg attctgttcg ccgagagcgt tgagacgcga
     3481 acccgacggt cgagcgcggc gtcttccggc gagatgatgc aaaaacgctg gtacgtctcc
     3541 ggccaaacgc aagatccgct ccggaacgtg ctggagctgc tttgccccga atgcagtctg
     3601 tgaatgcgag ttgcagcgct tcgcgcaagg gacgatgaat gaaacgttga cgctgcgagc
     3661 attgcgattt tgcgggggtg catttctagt tccgcgttcc ccgggcgaaa ccggaggcgg
     3721 caaacgcgtc acggtttcgg ttcgccgcgt ggcgcgcgct cgagcacggc gaacaccggg
     3781 cgatggtcgg accttccggc gtcgatcacc tctgcagaca gcgctcgcag ttcgggactc
     3841 gtcacgatgt gatcgaagcg ccgagacaag cgccccaggc tagtgttcca gctccacgta
     3901 tcgtcctggc tgaagtcggg cagggccgag tgtaaccccc gtgattcgag ccacgaaacc
     3961 gcgctgccgt gctcgctctc gttgaaaatg acgaaaagcc atgtgagcaa actcttcgcc
     4021 gaagcgcgca cgcagtgcac gctcccactc cggcgtagtc tcttgcagca acaccaggtc
     4081 cgaacgctgc gcagcaatcg cgtcgagcgt agcttcgtcc cccgcgatcc cgtagttgag
     4141 attgtacgac aacaccgcca aggtcgcttg gccgcgcggc ggcacgcccg gcgcttgcaa
     4201 cggagcgcta ccgcagccga ccgaggcgac tacggccggc agcaagacag cacaccaacg
     4261 cgccccccag cctcgctcac ctgtgtccgg cgccccaatc gacattcatg gcctccgcgc
     4321 ccagcatgag cccatcgccg ttaaaatcaa gctgtactgc gaatcacgac cacgagcggg
     4381 cgcgcggcgc tggctttcgg gttcgggtcc tctatggtag ccacacctgc ggcgccgtac
     4441 cattgcagtc gaacacttgc acgggggaat cattggctgc gccgggcgcc tcgacgcact
     4501 tcttgcggga ggggctgaat aggtccgcaa tcgttggacg tagctggccc cagctgtcgt
     4561 accagaactt ctggctgggg ctgccgttgc aatcagtgac agttagttgg gtacgattgg
     4621 ccgtgctcga agcactcagg cagcccggag cacgatccaa cggtcctgcc tcgcgtttga
     4681 cctctccgga gagctcgaag cgccaacgtt gacggcgcga gccatcgcag tttcgcagcc
     4741 gcacgccgct cgcgccagca tcgtgcaggc agcgcccctg atagccttgc acatctccgt
     4801 agtcgagcgc cgtgattcgg ctctgccgat acaggtagct cccgtgggac gcggagaccc
     4861 aggtttcctc gaacacggca gacgtcgatt cggtcaacgt ctgctggatc caggcgatgt
     4921 tgtcctcgat gagcgggcct tgatgccgcc tcgtcgagag cgggctccac ggcaagctca
     4981 tgaatcggcg gccgctgtgc acagcgaact cgaggaaatc tgcgcttgat tcggttccac
     5041 gaaacagcaa atagggtgat ccgctgtccc cattgcaggt atgcacggag tcgatcccag
     5101 cactcgcaaa gtgcgaattg gcagcgttgg gatccacgtg aagattcgaa ccctgtctca
     5161 agaggccgat gcctgtgtcg gcaccgtcct cccggtcaca gtcatcgttg ttgttgcccc
     5221 aacccgcgag aaacatctcg ttcggttcgt cgctatcggc ccaaggccgg cgtggatccg
     5281 catacagctt ggccttgccg atggccgtga gattcaggcc cgaacttgca agatagagga
     5341 gccccacgtc gtgcggcgcg tacgagctct gggtgtggtc cgggtgcgcg agagggacgg
     5401 ctctcccgtc gaagatcctg gtccgctccc cagcgctgtt cgcaaagaag acctcgatga
     5461 actcttcagc gtccaaaggc tccacgcaat gcgcggcggt gagcagccag tgctcgttca
     5521 agacgctggc gctgcaagca acgccaaaac ttctccgccc ccaacccacc gaccacctcg
     5581 gtgtcgttgt aaaggtcccg ccggcgaccg ctcgctgtgt ggacacgatc gattcatcct
     5641 cggattgcgc agcgctagcg caaccgaaca cgagggccag catgccgagc gcgacacctc
     5701 ctctggctgc atcgaaaccc gagcgaggca tgcgtgagtg acgtgacgag ggggactcga
     5761 gtggggacat acagctcctc cgtgcaccca gtgttccgca tttcagccag cgattcgaga
     5821 tttcttactc ggtgtatgcg actacagcaa tcaaatgctc atacgccagc tcgctcgagt
     5881 ggaaaatgaa ctattgaatt cctaatccaa ccgatccccg gaacggcgga gatcgctggc
     5941 gcgtggaagg cgagtttgcg ccgctgcccg atactgtgga gtcactcacg ggcggtggcg
     6001 caatctgtcc gaatcgcgaa gagcatgagt ccggacgcgc ctacgagagc agcaccgtga
     6061 tcttcgcacc gcgtcgccac gtcgagtgcg agatcgccct gacgccactc ttgaagcgaa
     6121 gcatcgattc gctttgcttc atcggagtcc ggtgcgatcg tcaccaatca cttcctccga
     6181 ctcgtcgcga tggccttggc ggtcaggagg ccacggcgcg agtagaggtc gagcaacacg
     6241 gccaggtacg tccagccctc ggcagtccag atgtacgtga tgtcgcccgc ccaaacttga
     6301 ttgggcgcag ctgcgttgaa gtccctttgt agaatgttgg gggcgaccgg cagcttgtgg
     6361 ctcgagtcgg tagtcttgcg gaagcggcgg cgtgggcggg ctcgaattcc gttgttccgc
     6421 atcaaccgag cgactcggtg cttgcctgct gctcgattgc acgcgaagtt gaccacccgt
     6481 ttgcaacgaa gttgaccacc cctgttcagg tgaagttgac cacccgtt
//
LOCUS       DASTKU010000555         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1669690, whole genome shotgun sequence.
ACCESSION   DASTKU010000555 DASTKU010000000
VERSION     DASTKU010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669690"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1794
                     /locus_tag="VFQ61_19240"
     CDS             103..1794
                     /locus_tag="VFQ61_19240"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9956644.1"
                     /translation="MLSLACGGSKPTDYSSSGGTGQQSGGNGGAGSWAGSNAGGRSTS
                     TGASGESGQAMGGATFGGASSSTNGGQEHSGGAGASSSTAEGGHSEPGGAGGEPTTTG
                     GATSQAGAPGHDDPDVEPSGPVPVLWLTIPGVPNPDDIDGDEATGFMRVIEAHDGTHA
                     DPAGFATAPASLETPISIHVRGKSSQGFAKKNYALEFHQQNGDQVKLKFLGMPKESDW
                     VLHGPYPDKTLMRNALVYWIAREMYRLPDGKIEAGRWSPRTRFTELYMNGQYWGVYVA
                     IERIKADADRVDVDRPAADAQSGDVSGGYIIRREGGGDGENNDWVSRVDQLVYTHHYP
                     KPKDLSPAQTEFIRGYFDSFESMMLEDYWNDPSRGYPAWLDVTSTLDYFLAMEWTNNV
                     DGYFKSVYMVKHAQSQGGRLSFGPLWDFDIALGNADYRNGDKVTNWVHNTTGTEASPY
                     KPPGEVTFIPPYVRRLWGDEGFRLALRCRWEALRAGALSHDPIEAKLDAWAARLSDAE
                     RRDHMRWPVIGKRIWPNPVAWISYEAEIVYLKRFMRQRFEFLDVELAKLGPGECL"
     gene            1900..3450
                     /locus_tag="VFQ61_19245"
     CDS             1900..3450
                     /locus_tag="VFQ61_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HET9956645.1"
                     /translation="MQTGDSFQGRISSVGRTPLSSVGRAIPLSVAMLSVTLLQGCGGD
                     SEPRNAAKGGTSSGGSTAQGGSRVDTSSSSGGSGTTSGGAKTSDGGSSAGGSTPRSSG
                     GSPPQGGADSGGGESGATDGGAGESSGGSGGSPPDPNFGGSAGADGGAGGEAGAPVIE
                     PLDTNPNLVRTLGGEPTSYMVNTNMGGSIFHDVCPHDQVIIGFGYDNYEDFLNGLTVR
                     CGSIRVKSIDSALKIDVVPGVTLFQRGGGDPKDSGDVMCPAGHVMVGFKGIFNDWIRK
                     ITFSCAPVSVGDAEAGYKVTVGDPVELTTVGKDSNPNPGFPLQETPYIACPAGQIARG
                     TKMNAGFWMEGLGFYCGAPKVSFGLGNACRNNSDCTGLAACDVASKTCKAVACNAPSN
                     CSCGQLDGKVYSFCAETTDSTYANAGSSCADQSRRLAWAKDGFVNGWQRYAALEAAVG
                     SPYWIGLDSLSAVGVWSWANSGGPANFTDWHQNEPSNPASNRCAFVDADGAWSASPCN
                     QPRAYVCE"
     gene            complement(3508..>3779)
                     /locus_tag="VFQ61_19250"
     CDS             complement(3508..>3779)
                     /locus_tag="VFQ61_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HET9956646.1"
                     /translation="LSELWLGANDISTEKRWVWADAKGTQFWQGGPLTVDGAPVGGRY
                     TNWLSGEPNDAYGVPPGEDCAILRTDERWEDQACDLEKAFVCEAL"
BASE COUNT          723 a         1101 c         1250 g          705 t
ORIGIN      
        1 ttgcctccag gaaactcaat gactgcgtct tcggtcgcct cggacaatct cactccgagg
       61 tggggagtcc gccgcttgcg gtaccgcgct tgttttgtgg ctatgctttc gctcgcatgc
      121 gggggctcga agccaaccga ctactcgagc tcgggcggca ccggtcaaca atcgggcgga
      181 aacggtggtg cgggcagttg ggcaggctcg aacgcgggcg ggagaagtac gagtactggc
      241 gcttcgggcg agtcgggaca ggcaatgggc ggcgcgacat tcggtggcgc gtcctcgagc
      301 acgaatggtg gtcaagagca ctccggtggc gccggtgcgt cttcgtcgac ggccgagggc
      361 ggacactcgg agccgggcgg tgcgggcggt gagcctacga caaccggagg ggctacctcg
      421 caagccggag cacccggcca cgacgatccg gatgtggagc cctcgggccc cgtgcccgtg
      481 ctttggttga ccataccggg cgtgccaaac cccgacgata tcgacggcga cgaggcgacg
      541 gggttcatgc gcgtcatcga agcgcacgac ggaactcacg ccgatcccgc cggttttgcg
      601 acggcaccgg cctcgctcga gactcccatc agcattcacg tgcgaggcaa gagctctcaa
      661 ggtttcgcga agaagaacta cgctttggag tttcaccagc agaacggcga tcaggtgaag
      721 ctgaagttcc tcgggatgcc caaggagagt gactgggtcc tgcacggccc ctatcccgac
      781 aagacgttga tgcgcaacgc gctcgtgtac tggatcgcgc gcgaaatgta ccgtctaccc
      841 gacggcaaaa tagaagctgg gcggtggagc ccgcgaactc gattcaccga gttgtacatg
      901 aacgggcagt actggggtgt gtacgtcgcc atcgagagga tcaaggcaga cgcggaccgc
      961 gtcgacgtcg atcgacctgc cgccgacgcg cagtcgggcg atgtgtcggg tggctacatc
     1021 attcggcgag agggcggcgg tgacggcgag aacaacgatt gggtctcgcg cgtagaccag
     1081 ctcgtctata cccaccacta tcccaagccc aaagatctgt ctcccgcaca aaccgagttc
     1141 attcgtggct acttcgactc tttcgagtcg atgatgttgg aagactattg gaatgatccg
     1201 tcgcgcggct accccgcttg gctagatgtc acgagtacgc tggattactt cctggccatg
     1261 gagtggacga acaacgtcga cggctacttc aagagcgtct acatggtgaa acatgcgcag
     1321 agccagggag gccggctctc cttcgggccg ctttgggact tcgatattgc cctcggcaac
     1381 gccgactacc gcaacggcga caaggtcacg aattgggttc ataacacgac cggtaccgaa
     1441 gcctcgccat acaagccgcc cggcgaggtg acgttcattc cgccttatgt ccggcgtctt
     1501 tggggcgatg aaggtttccg cctcgcgctg cgctgtcgct gggaggcgtt gcgcgccggc
     1561 gccctctcac atgatccgat cgaggcgaag ctggacgcct gggctgcgag attgagcgat
     1621 gcggagcgcc gcgaccacat gcgttggccg gtcatcggaa agcgaatctg gcccaatccc
     1681 gtggcgtgga ttagctacga agcggagatt gtttatctca agcgcttcat gaggcagcgc
     1741 ttcgagttct tggatgtcga gctcgccaag ctcggaccag gtgagtgttt gtagtcgttt
     1801 aatccaagtg gtttaggcga gttcggctgg acatgaacct aacggaactc gacagatttg
     1861 tgacgcgctg ctgacctcga gatgttaatc tgccgcccca tgcagacagg ggattcattt
     1921 cagggccgca tctcgtccgt cgggcgtaca ccactatcga gtgtgggccg cgccataccg
     1981 ctctcggttg ccatgctgtc ggtcactctc ctccaaggct gcggagggga cagtgaacca
     2041 cgcaatgcag ccaaaggtgg tacaagttcc ggcggcagca cggcacaggg cggcagcaga
     2101 gtagacacct catcttcctc gggtggctcg ggcactacga gcggaggagc caaaacgagc
     2161 gatggcggca gtagcgcggg cggtagcact cccaggagca gcggaggaag tcctccgcaa
     2221 ggcggagccg attcgggcgg tggcgagagc ggagccaccg atggcggagc gggagaatcc
     2281 agcggcggga gcggcgggtc gccgcctgac ccgaatttcg gcgggagcgc cggcgccgac
     2341 ggaggtgccg gtggcgaggc gggcgcgccc gtgatcgagc ccttggacac gaatcccaac
     2401 ctcgttcgca cgctgggtgg tgaaccaact agctatatgg tcaacacgaa tatgggtggc
     2461 agcatcttcc acgacgtgtg cccccatgac caggtcatca tcggcttcgg gtatgataac
     2521 tacgaagact tcctgaacgg cttgacggtg cgctgcggct cgatccgagt caaatccatc
     2581 gactccgctt tgaagatcga tgtcgtgccg ggagtaaccc tgtttcagcg cggtggcggc
     2641 gaccctaagg acagcggaga tgtcatgtgt cccgcgggtc acgtgatggt gggattcaag
     2701 ggaatcttca atgactggat acgtaagatc acgttctctt gcgcgcccgt cagcgtcggc
     2761 gatgcggagg ctggctacaa ggtgaccgtg ggcgaccccg tagagctgac taccgtgggc
     2821 aaggactcca atccaaaccc aggctttccg cttcaagaga cgccctatat cgcttgtccc
     2881 gctggccaga ttgcgcgcgg aacgaagatg aacgcgggct tctggatgga gggtctgggc
     2941 ttctactgcg gcgctccaaa agtcagcttt ggcctcggca acgcatgtcg caataactcg
     3001 gattgcacgg gtttggcggc gtgcgatgtc gcgagcaaaa cgtgcaaggc ggtggcgtgc
     3061 aacgctcctt cgaattgttc ctgtggacag ctcgacggca aggtttattc gttctgcgcg
     3121 gaaacgactg actccactta cgcgaatgca ggctcgtcgt gcgcagatca gagccggcgt
     3181 ttggcgtggg ccaaggatgg gttcgtcaat ggttggcaac gctacgcggc gttggaagcg
     3241 gcggtcggga gcccgtattg gattgggctg gatagcctca gcgccgtcgg cgtctggtcc
     3301 tgggcgaact caggagggcc ggcgaacttc acggattggc accaaaacga gccctccaac
     3361 ccggcgtcca atcgttgcgc gttcgtggat gcggacggcg cgtggtcggc ctcaccgtgc
     3421 aatcagcccc gcgcctatgt gtgcgagtga gcccgcgtcg gtgagttacg gagtgtgact
     3481 gaggggccgg ctgccgagcc cggcccctta aagagcttca caaacgaacg ccttctccag
     3541 atcacaagcc tggtcttccc agcgttcgtc agtgcgaaga atggcgcagt cctcgccggg
     3601 agggacgccg taggcatcgt tcggttcacc gctgagccag ttggtgtaac ggccgcctac
     3661 gggtgcgcca tcgacagtga gggggccccc ttgccagaat tgcgtgccct tcgcatctgc
     3721 ccaaacccag cgcttctcgg tcgagatgtc gttggcgccg agccagagct ccgacagct
//
LOCUS       DASTKU010000556         4095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_331046, whole genome shotgun sequence.
ACCESSION   DASTKU010000556 DASTKU010000000
VERSION     DASTKU010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331046"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VFQ61_19255"
     CDS             <1..790
                     /locus_tag="VFQ61_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HET9956647.1"
                     /translation="STSADLALERGPHPAFAETRAAQGELQFDYWPQKPDDPERFEAL
                     RQRIRQQGLRNSLLIAVAPTATIAAIAGCSECIEPQVSNLFKRETLSGEFLQINRYLV
                     RDLENLGLFTEEFRDRLKLAEGSIQGMTDLPEHLRQLYRTAWEVPMRHVIDMAAERAA
                     FIDQSQSLNLFVESPNIGRLSSMYFHAWKRGLKTTYYLRSRPATRIAKATLTTPLPPS
                     VGIRSASASGDPTNHASATPGDSQSPMRAVVCSLENPETCEACQ"
     gene            787..1809
                     /locus_tag="VFQ61_19260"
     CDS             787..1809
                     /locus_tag="VFQ61_19260"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HET9956648.1"
                     /translation="MTSIITRPARLLDPGSCLSLRPLRYPVFYDMYRDAVRNTWSVDE
                     VDFAKDVDDLRRLGPAERHLVERLVAFFATGDSIVANNLVLNLFRHVNAPEARMYLSR
                     QLYEEALHVEFYLTLLDTYVPDPEARARAFAAVEHIPSIRQKADFCFRWIDSMHELRT
                     LNSRQERKAFLRNLIAFAAGVEGLFFFAAFAYVYYLRSRGVLHGLATGTNWVFRDESG
                     HMAFAFEVARVAREEEPELFDAELETEVLKMLEQAVQAETQFAADLVSGGLPGLSLTD
                     VRTYLLCVADQRLVQLGMRPQYGAKNPFSFLELQDVQEHTNFFERRVAAYQVGIPGEF
                     ALDAGF"
     gene            complement(1835..2275)
                     /locus_tag="VFQ61_19265"
     CDS             complement(1835..2275)
                     /locus_tag="VFQ61_19265"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HET9956649.1"
                     /translation="MHANAVTVYVLSVIVLFAKTLATITVQAVQRLRSRQFRYPEDAA
                     EWKGTAGPDNDVTQRAQNLLRNDAETQIFYLALGAAYALIGNWPPWAPVAFTVYPLSR
                     LVHAYFFLRPRQPHRNRAFSLGLIVLALLAGHLAYVCIVRLAAD"
     gene            complement(2462..3355)
                     /locus_tag="VFQ61_19270"
     CDS             complement(2462..3355)
                     /locus_tag="VFQ61_19270"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET9956650.1"
                     /translation="MALVKLLIDVPTQLAEDVGQALIELGAGGVEEQDGDRGARLIVY
                     GSDRAELSALAERARDALSEMGLDEDSGNVSLRIEVDEQSDWDTAWTRHLEPQALTPH
                     WIIQPFWDETQPPENMDCIFIRPTLAFGDGAHATTRLAAQAVERFCLALPDASVLDIG
                     TGTGVLSLVAALSGARTVVGVDIDAVALAAARENARLNDLEPQIVFQDASAELSTGFD
                     LVVANLEPRALISDAARIADRARDARELVVTGFLSSQAPDVADAFSKYGFVPLGQVEE
                     DEWSLLVLGRRGPSSANPDEA"
     gene            complement(3355..>4095)
                     /locus_tag="VFQ61_19275"
     CDS             complement(3355..>4095)
                     /locus_tag="VFQ61_19275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET9956651.1"
                     /translation="TSLKTIDQTLTLPPVWIPNPPAFENYEKTVHYIPFWQYTLNTLL
                     VCVLSAVGTTFSSALVAYSFTRLEWPGRNLFFALTLGTMMVPFPVLMVPLYTVFRSLG
                     WIGTLKPLWAPAFFGSAFNVFLLRQFFLRIPKSLPEAMMLDGASEWRIFVHTYLPLSK
                     AALAVAAFFQFLYSWNDFLGPLLYLTDQSTFTLSLGLQFYHGQHGGTQWHYLMAASFL
                     TTLPLIVLFFFLQRAFLSGLSFMKNVDA"
BASE COUNT          812 a         1209 c         1341 g          733 t
ORIGIN      
        1 cagcacctcg gccgatttgg cgctcgagcg ggggccgcat cccgccttcg cggagacgcg
       61 cgcggcgcag ggcgagctgc aattcgacta ttggccgcaa aaacccgatg atcccgagcg
      121 tttcgaggcg ctgcgccagc gcatccgaca gcagggactg cgtaattccc tgctgattgc
      181 cgtggctccc acggcgacca tcgccgcgat cgcgggctgc tcggagtgca tcgagccgca
      241 ggtctcgaat ctgttcaaac gcgagacgct gtccggggag tttctacaga tcaatcgata
      301 tctggtgcga gatctggaga atcttgggct atttacagag gaattccgcg atcgtctgaa
      361 gctcgccgag ggctcgattc aaggcatgac cgatctgccc gagcacctgc ggcaactcta
      421 tcgcaccgcc tgggaggtgc cgatgcgaca cgtgatcgac atggccgctg agcgcgcggc
      481 gttcatcgac caaagccaat cattgaatct gttcgtcgag agcccgaaca tcgggcgcct
      541 ctcctctatg tatttccatg cttggaaacg tggcctgaag accacctatt atctgcgctc
      601 acgccccgcg acgcggatcg ccaaggcaac gttgactacg ccgcttcccc cgtcggtcgg
      661 aattcgaagc gcgagcgcca gcggcgaccc tacgaatcac gccagtgcga cgccaggtga
      721 ttcgcagagc ccaatgcgcg ccgtggtgtg ttcactcgag aaccccgaga cgtgtgaggc
      781 atgccaatga catccatcat cacgcgcccc gcccgcctgc tcgaccccgg ctcgtgtttg
      841 agccttcgcc cgctgcgtta tccggtgttt tacgacatgt accgcgacgc ggttcgtaac
      901 acttggtcgg tggatgaagt ggacttcgct aaggacgtgg acgacttgcg gcgcctgggc
      961 cccgccgaga ggcacttggt cgaacgcctc gtggcgttct ttgcgactgg cgacagcatc
     1021 gtggccaata acctggtgtt gaacctgttc cgtcacgtca acgcgcccga agcgcgcatg
     1081 tacttgtcgc gacaactcta cgaagaggca ttgcacgtcg agttctacct gacgctcctc
     1141 gacacctatg ttcccgatcc cgaggcgcgg gcgcgagcgt tcgctgcggt ggaacacatt
     1201 ccctcgatcc gacagaaggc agacttctgc tttcgatgga tcgattcgat gcacgagctg
     1261 cgcaccttga actcacgtca ggagcgaaaa gcctttctgc gcaacctgat tgccttcgcg
     1321 gccggggtag aagggctgtt cttcttcgcg gcgtttgctt acgtgtacta cctgcgctcg
     1381 cgcggagtgc tgcacggttt ggcgaccgga accaactggg tgttccgcga cgaaagcggg
     1441 cacatggcct tcgcgttcga ggtggcccgg gtggcaaggg aagaggagcc ggagctgttc
     1501 gatgccgagc tcgagacaga agtcttgaag atgctggaac aggccgtgca agccgagact
     1561 cagtttgcgg cggatctggt cagcggtggt ttaccaggct tgtcgttgac ggatgtgcgc
     1621 acgtatctgc tgtgcgtcgc cgatcaacgg ctggtgcaac tcgggatgcg cccccaatac
     1681 ggcgcgaaga atccgttctc cttcctggaa ctgcaggacg tgcaggagca tacgaacttc
     1741 ttcgagcggc gtgtggcggc ctatcaagtg ggcattccgg gagagttcgc gctcgacgcg
     1801 ggcttttgaa cggcaaatgc ggccctgaag cgcatcaatc ggccgcaagc cgaacgatgc
     1861 acacgtacgc caagtgaccc gcgagtaacg cgagcacaat caagccgaga gagaaggccc
     1921 gatttcggtg gggctgccgt ggtctgagga agaaataggc gtgcaccagt cgcgagagcg
     1981 ggtaaaccgt aaacgcgacg ggtgcccacg gtggccagtt gccgatgagc gcgtaggccg
     2041 cgcctaaagc caggtagaaa atctgagtct ccgcgtcatt gcggagcagg ttttgggcgc
     2101 gctgggtgac atcgttgtcc gggcctgccg tccccttcca ctcagccgcg tcctccgggt
     2161 agcgaaactg ccgggaacga aggcgttgaa ccgcttggac cgtgatggtc gccaaagttt
     2221 tggcaaacag gacgatcacg gagagcacgt atacggtaac cgcgttcgcg tgcatggccc
     2281 cgccttagag cgcgaacggt gtacgtacgc aagcgtggat ggaactcggg gcgccggcgc
     2341 ggtgtttttg ggaagagtcg tgcaagcccg cctgcgcacg agcgagacgg gacggacggc
     2401 gcgcacgtta tagcgcggcg cgctcggaag ataggatggc gcgctcggaa gagcagacgc
     2461 tctaggcttc gtctggattc gccgagctgg gcccgcgccg tccgagcacg agtaagctcc
     2521 actcgtcttc ttcgacctgg ccgagcggca cgaagccgta cttggagaag gcgtccgcga
     2581 cgtcgggggc ctggctcgag aggaagccgg tcaccacgag ttcgcgtgcg tctcgggcgc
     2641 gatcggcaat gcgcgcggcg tcactgatca gggctcgtgg ttctaagttc gccaccacga
     2701 gatcgaagcc ggtcgagagc tcggccgacg catcctggaa gacaatctgc ggttccagat
     2761 cgttgagccg ggcgttctcg cgagccgccg ctagagcgac ggcgtcgatg tccacgccga
     2821 ccacggtgcg agccccgctc aacgccgcca cgagcgatag aacgccggtg ccggttccaa
     2881 tgtcgagcac gctggcgtcg gggagcgcca agcagaagcg ctctacggct tgggccgcca
     2941 agcgcgtggt ggcgtgcgcg ccgtcgccga acgcgagtgt cggccggatg aagatgcaat
     3001 ccatgttctc cgggggctgc gtctcgtccc agaaaggctg gatgatccag tgcggagtga
     3061 gtgcctgggg ttcgaggtgg cgcgtccagg cggtgtccca gtccgactgc tcgtcgactt
     3121 cgatgcgtaa ggacacattg ccgctgtcct cgtcgagccc catctcactc agcgcgtccc
     3181 gggcgcgctc agccagcgca gacagctctg cacgatcgct gccgtagaca attagacgcg
     3241 cgccgcggtc accgtcctgt tcctctacgc caccggcacc gagctcaatc agggcttggc
     3301 ccacgtcttc ggcgagctgg gtcggaacat caatcagcag tttgacgagc gccatcacgc
     3361 atccacattt ttcatgaagc tgagcccgct gaggaaagcg cgctgcagga agaagaacag
     3421 cacgatcagg ggcagcgtgg tcaagaacga ggcggccatc agatagtgcc actgcgtgcc
     3481 gccgtgctgg ccatgataaa attgcaggcc cagagagagg gtgaaggtac tctggtcagt
     3541 gaggtaaagc agcgggccca agaagtcgtt ccaggaatag aggaactgga agaaggccgc
     3601 aacggccaga gcggctttgg agagcggaag ataggtgtgc acgaaaatgc gccactcgga
     3661 cgcgccatcc agcatcatgg cctcgggcag gctcttcggg atgcgaagaa agaactgccg
     3721 caggagaaac acgttgaagg cgctgccgaa gaaggccggg gcccagagcg gcttgagcgt
     3781 gccaatccag cccaggctgc gaaacacggt ataaagaggc accatgagca ccgggaacgg
     3841 caccatcatg gtgccgagcg tgagcgcgaa gaacaggttt cttccgggcc actcgagccg
     3901 cgtgaaactg taagcgacga gggcactcga gaacgtggtg ccgactgccg aaagcacgca
     3961 caccaacagg gtgttcagcg tgtattgcca aaacggaatg tagtggacgg tcttttcgta
     4021 gttctcgaac gcgggcgggt tcgggatcca aacgggcggt agggtcaggg tttggtcgat
     4081 agtcttgagg gacgt
//
LOCUS       DASTKU010000557        11094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1224458, whole genome shotgun sequence.
ACCESSION   DASTKU010000557 DASTKU010000000
VERSION     DASTKU010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11094
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224458"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /gene="tatB"
                     /locus_tag="VFQ61_19280"
     CDS             complement(<1..361)
                     /gene="tatB"
                     /locus_tag="VFQ61_19280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HET9956652.1"
                     /translation="MFGFSFGELMVICIVALVAVGPKRLPSLLGTLGQWIRKLRNMVH
                     DMRAQSGIDEILRAEGLQGGINELRSLMRVQHTQFFQPDPPQPPRPAPSSDVGTPEVE
                     SGAAAPPPVAGLTPAEDP"
     gene            541..1542
                     /locus_tag="VFQ61_19285"
     CDS             541..1542
                     /locus_tag="VFQ61_19285"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET9956653.1"
                     /translation="MIAPTVFAYLLVIKSMDRFEPEPLWLLGMAFAWGAVVSTLLAIL
                     GNALGESALSAALGSGRGDALLQASTASFVAPLVEETTKGLGLLVLWALSALFLNEID
                     GALDGAIYGGVVGLGFTLTEDVLYVSSAGSQGGAAAFFGTFVLRTVVSGLGHASFTAM
                     TGLGIGVAAESRSGLMKLIAPVGGWCAAVGLHFLHNFLVTFLYDGGTGLVVKLLAFWL
                     FDALFFVVIVVLALRDRAIVLRGLMEEAGRLLHPKELARTTSHWMLLPLWNFFTLSGG
                     QSGYRRARRKQLDLIELSFLKQRRARGQSGADLTRREHELRSRIHTANQAGVFIGPR"
     gene            complement(1593..1718)
                     /locus_tag="VFQ61_19290"
     CDS             complement(1593..1718)
                     /locus_tag="VFQ61_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956654.1"
                     /translation="MTIEHLIFIPGVLLVGVTLGYVLGARAVRAEIERLKRRARE"
     gene            complement(1760..2056)
                     /locus_tag="VFQ61_19295"
     CDS             complement(1760..2056)
                     /locus_tag="VFQ61_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HET9956655.1"
                     /translation="MDDQTNSKPSAQVGVPGDRSTEFAPVTGGPELTSAGTLLVVAYL
                     AMWAVLMVMIWLGWRRQQRIDQRLQELSRVLGTAPATDVSGPSGATVRAATAKL"
     gene            complement(2196..2897)
                     /gene="ccsA"
                     /locus_tag="VFQ61_19300"
     CDS             complement(2196..2897)
                     /gene="ccsA"
                     /locus_tag="VFQ61_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET9956656.1"
                     /translation="MRSRAFDVLAALTAGVFLFLIAFVFFKVPTAQLQAGGLAQKIFY
                     FHAPAGYAMYLCGGVCFIASALYLAGPSDARDAWAKGAAECAVAFGLMMLISGPLWAK
                     KAWGVYWTWDPRLTTLLLSVLIYIAIVVLRAFGGSGEPERRFAAALGVLGTVNLPIIH
                     YSVKKWGGNHPTVITQGGGGLRDPDMRLALMMGFLAMTLLAVLLVWSRARLALSSAKL
                     ARLEEEAAARGLLEA"
     gene            complement(3078..4586)
                     /locus_tag="VFQ61_19305"
     CDS             complement(3078..4586)
                     /locus_tag="VFQ61_19305"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9956657.1"
                     /translation="MATGRRFASILVPGLLVLNCISRSALGQVNTAPMPPAGQAGKPA
                     ANPAPNAGQATSESAAPAAGAEPTLPKLEDPMLVPPPAAQQTLSTWQQALTILRQNST
                     ALKSSRARIQQATAQRRLALAPALPTLTANGTATQHLLTGEGCTTINPGTTEAATVCG
                     KIPNPSGYYVAGLNLRVPVLAPRAWYDQATARDVIENSKLDQKDVERREIARVAATIV
                     NVVTQERLAEVSRVALSSALTTLDLSRRRAALGAASRIDVLRIEQEVSDSRAQVVAAT
                     ENLLRSREELGNALSSSEPYGITPDIRLDNLAEQAAGVCQREANVELRPDVRAASAAT
                     HVAERNIKSVDYAFIPTLDGVSTLSYASTPLRSENREHVSWTIGAVLTWQIYDGGVRY
                     GTRDARRADLELSQQNLSDVRRRATIEIRQALRGLTVAQQNLEVAQKARDLAAETARL
                     TRIAYLNGTGTSFDLVDSARRLRSAELDLANRELSVLQSKLTALLALATCKV"
     gene            complement(4770..5384)
                     /locus_tag="VFQ61_19310"
     CDS             complement(4770..5384)
                     /locus_tag="VFQ61_19310"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9956658.1"
                     /translation="MPLELERMVGDVKWAKAHRLPARELKAMLRRLVAAAPEGSAERR
                     FARLELAAVLVESEPWLAARLARDALRAEGEWRVGEDDRALGVLGIAHMLLGNFRSAR
                     EAFRRAIEVAPDSAEHRHNLGHVLDVAFNRPLSALPHLKLAHQLMPEEPEIASSLAHA
                     LLGAGREAEALRHLVRVFEGDSKRAQATLQAWRTQRRVKSHRAK"
     gene            complement(5421..8054)
                     /locus_tag="VFQ61_19315"
     CDS             complement(5421..8054)
                     /locus_tag="VFQ61_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9956659.1"
                     /translation="MSSEPDTSALPTFDVLPLSQDLRKAVDELGYVHPTPVQRAVFEP
                     ASRGRDLAVQARTGTGKTAAFGMPLIDAIVRRKEAEVQALVLCPTRELALQVTRELEA
                     LARYRATRIVAVYGGAPMQAQIDRIRDGAQIVAGTPGRVLDHLERGTLNPKSIRAVVL
                     DESDEMLSMGFLPQITEIFSHLPESRQTLLFSATLPPDIRRMAETRLREPEFITLSGD
                     HIGALEIRHFVYLSRSDKLAELVQVIETENPESAIVFCNTRDETKRVSAALVQQGYAA
                     DWLNADLPQPDREKVMAATREGRLKFLVCTDVASRGIDISHLTHVINFDFPESAENYV
                     HRTGRTGRAGRTGTAISMIEPSDIGNLYLLRLTYKLKPIERQLPTAREVKTRAEADLV
                     RWFVEGFAKRRVRDDDLALARRLLTHEQAEVVVAGLLRDHLGALPEAPEAAAAARRAA
                     VVPVAAGNATPTSQASRPREPSGGRGKIAERSATAERSATVERSATVERSGSSRNGLE
                     RRRRDNLERESSADDRLIGKGAQGDRTPAEEEELGGPSEYAGERRTPEGLARATEARP
                     ARENLDERTGLSRRSERRADRRDGFESRDVERRNRRAGEPGEPESRSNASESGDTSLA
                     ARRTNEGRESREAVELGDERAAPARDDRVAHDERAARPERAPRNEPNRSERRPYEERR
                     GFDGRGAKRRERTTNLSSGTPRYTVSDLPELTEVAPSPRPSPETDLSEISDEPETMRR
                     GQLRQEPAQPEFAPKLDSDEVEATSSSSQSNPPTDPLALARPGDWVELYASLGRRQGV
                     KASDYERLFEARGVPAHAIGYIRVRHRNAFIAVERADLESVLGAFEGAQIAGRPVKAE
                     LSRGGGAERSGQKEEENEEPV"
     gene            8149..8421
                     /locus_tag="VFQ61_19320"
     CDS             8149..8421
                     /locus_tag="VFQ61_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956660.1"
                     /translation="MLFCIAKRIQIGKSGESSGVTPTDFVQSLSALPFWQKAAAKAVE
                     RGALAVRFLSRVRARTPHRGFEFEDRTPTRTASGHGFGRENLPKNL"
     gene            8751..9776
                     /locus_tag="VFQ61_19325"
     CDS             8751..9776
                     /locus_tag="VFQ61_19325"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET9956661.1"
                     /translation="MTLQAATSVLADTAQATLAGPRAMDRLEAIQSLLSDDLRWIEES
                     LRQVCDDGPAPAVQAAQHLVSRGGKRVRPLSMLLSAACFRPPSDVVRELAVVSELVHS
                     ATLLHDDVVDEGDERRGAMAARLVWGNAVSVLGGDLLLVHALERTARLAPELMGDLIA
                     TLRRLVDGEIVQLRGRNELDVSETTYERILRDKTASLFAWSTRTGAHLSGASRSDQDR
                     LSVFGERLGLAFQLVDDVLDYSGDASGKSLLTDLREGKLTLPLVLTVAKVPELIGPLK
                     RIYAGDREPVTLVSRAVQECGALDEVRRRATHHTHEAVRSLEAVQPSPARRVLEQVAS
                     ELARRAA"
     gene            complement(9892..10887)
                     /locus_tag="VFQ61_19330"
     CDS             complement(9892..10887)
                     /locus_tag="VFQ61_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9956662.1"
                     /translation="MDQFSAHLDRGWDLVQRGDPRGAEVSARRALEIDSQSPEAYNLL
                     GYVAALKGEYEEAIDHYRQAVALDDTYLEAMLNAAEVYIHPLGEFDDAINMCDQALDL
                     AETDDEIVDALLLKFDALLGKGELDQAALVCGQFPPGPFENPNHVFLVGRAYYEIGDI
                     DHAEPLIDEAVKQNARNAEAYYYLGLVRDERGDTHAATEAFLRSRELDLETPAPPWTL
                     SSETFALTAKRAFQALDERLRVYLREEEIYVCDVPGVEVVVDGVDPRALLLIDGVGAA
                     GNSMNARVFVYQRNVERLAGSVEQVESELQLALEREITATFLEQETSGDRNLTLN"
BASE COUNT         1873 a         3587 c         3559 g         2075 t
ORIGIN      
        1 acggatcctc cgccggcgtg agcccagcaa ccggaggtgg ggcggcggct cctgactcta
       61 cctcgggcgt tccgacgtca gagctcggtg caggacgggg cggctgcggc gggtcgggtt
      121 gaaagaactg cgtatgctga acgcgcatca gcgaacgcag ctcgttgatg ccgccttgca
      181 ggccttccgc acgcaaaatc tcatcgatcc cgctctgtgc ccgcatgtca tggaccatat
      241 ttcgcaattt gcggatccac tgcccgagcg tcccgagcaa gctcggcagg cgcttcgggc
      301 cgaccgccac gagcgcgacg atgcagatga ccatgagctc gccgaacgag aagccgaaca
      361 cccccgcaaa ctagcgttcc tcagacacca aagcaacccg ctggggccgc gttacgcctt
      421 cggattgcgg cacaatcttc cggctttccg ggcggatggc gcgcggtacc ccgaacgctt
      481 ggttctcggc cccggcagag ttattggaag cgcgctttga tttggattct cgctctcagc
      541 gtgatcgcgc ctaccgtttt tgcgtacttg ctcgtcatca agagcatgga tcgcttcgag
      601 cccgagccgc tctggttgtt ggggatggct ttcgcgtggg gggctgtggt gtccacgctg
      661 ctggccatct tgggcaacgc tctcggtgaa agtgcgctga gcgcggcact cggatcaggg
      721 cgaggggacg cgctgctcca ggcttccacg gcctctttcg tggccccttt ggtcgaggag
      781 accaccaaag gtttggggtt actggtgttg tgggctcttt cggcgctgtt cctgaacgag
      841 atcgacggag ctctcgacgg ggccatttac ggcggagtgg ttggactcgg cttcactctg
      901 accgaagacg ttctgtatgt gagcagcgcg ggttcacagg gcggcgcggc cgcgttcttc
      961 ggaacgttcg tattgcgcac ggtcgtctcg ggcttggggc acgcttcgtt cacggccatg
     1021 accggcctag gcatcggcgt ggccgccgag tcccgctctg ggcttatgaa gttgatcgcg
     1081 cccgtgggcg gttggtgtgc cgccgttggc ttgcacttct tgcataactt tctggtcacg
     1141 tttctctacg acggcggcac gggcttggtg gtcaagctgc tggccttctg gctattcgac
     1201 gccctgttct tcgtggtgat cgtcgtgctc gcgctacgcg accgcgccat cgtgctccgc
     1261 ggactcatgg aagaagcggg ccgcctgctt cacccaaagg aactggcgcg caccacgagc
     1321 cactggatgc tgctgccgct ctggaacttc ttcacgcttt cgggaggcca gtctgggtac
     1381 cgccgtgcgc gtcgcaagca gctcgacttg attgagctct cctttctcaa gcagcggcgc
     1441 gcgcgagggc aatcgggcgc ggacttgacg aggcgcgagc acgaactccg gtcacggatc
     1501 cacaccgcca accaagccgg tgttttcatc gggccgcgct gacccaggcg caggctagaa
     1561 ggcggcctcg cttggcgcgc gtcgggcgtg cgtcactccc gcgcccgacg cttcaagcgc
     1621 tcgatctcgg cccgcaccgc tcgagcgcca agcacgtagc ccaaagtgac gccgacgagc
     1681 agcacgcccg ggatgaagat caaatgttcg atggtcatgt gcgttcgaat ggctctgggc
     1741 ccgcggagcg ctccagtggt tagagcttgg cagtggccgc gcgaacggtc gcgccgctag
     1801 gccccgaaac gtctgtggcg ggcgcggtcc cgagcacgcg agacagctct tgcaaacgct
     1861 ggtcgatgcg ctgctggcgt cgccacccga gccagatcat caccatcaac acggcccaca
     1921 tggccagata tgcgacgacg agcagcgtgc cggcgctcgt gagttcgggc ccacccgtga
     1981 cgggagcaaa ttccgtcgag cggtccccgg gcacgcccac ctgagctgac ggtttcgaat
     2041 tcgtttgatc gtccacgtcg gtattcctag tgcgagtgat agcaagtact acgaaaacta
     2101 tcgggcggga gcggcaggac atgccgcggc gtgaaccgcc cgcgtcccgc cctcgtaaga
     2161 cgcgagcagc gaggcaaacg gtgtggcttc ctgcgctatg cctcgagcaa accgcgagcc
     2221 gcggcttcct cttctagtcg cgcgagtttg gcgctcgaga gcgcgagccg agcacggctc
     2281 cagaccagca acacggccaa gagggtcatg gccaagaagc ccatcatcaa ggcgagccgc
     2341 atgtccggat cgcgcaagcc gccgccacct tgggtgatta cggtgggatg gtttccgccc
     2401 cacttcttca ccgaatagtg gatgatcggc aagtttaccg tgccgagtac gcccaatgcc
     2461 gcggcgaagc gccgctcggg ttcgccgctt cctccgaaag ccctcagcac cacgatcgca
     2521 atgtaaatca gcacgctgag cagcagcgtg gtgagccggg gatcccacgt ccaatacacc
     2581 ccccacgcct tcttggccca cagcggtcca ctaatcagca tcatcaggcc gaatgcgact
     2641 gcacattcgg cggcaccttt ggcccaggcg tcgcgcgcat cgctggggcc ggccaaatac
     2701 aatgcgctcg cgatgaagca aacgccgccg cacaggtaca tcgcgtagcc ggcgggcgcg
     2761 tgaaagtaga agatcttttg cgccaggccc ccagcttgca actgcgccgt gggcaccttg
     2821 aagaacacga aggcaattag gaacaggaac acgcccgcgg tcagcgcggc caacacgtcg
     2881 aaggctcggg aacgcatcgt tccgaaagcg tagtctcgag cgcgtccact cacaacgctt
     2941 caaccccaac ttctgccagg aagcttgtcg attcgaactt tctcgcgtct ccaaaccgcg
     3001 ctgagcgcga gatcccattc gggtttcgaa gccgaggcgc ccggagcgcg gcgtcgaccc
     3061 gggagcgtta cagccgctca gactttgcag gtggcgagcg ccaatagggc ggtcagcttc
     3121 gactgcagaa ccgacagctc tcggttggct aggtccaact ctgcggagcg caaacgtcgc
     3181 gccgagtcca cgagatcgaa gctggtgccg gtgccgttca ggtacgcgat ccgggtcaag
     3241 cgcgccgttt ccgccgccaa atcacgtgct ttttgggcaa cttcgaggtt ttgctgggcc
     3301 accgtcaaac cacgcagggc ttggcggatc tcgatcgttg cgcggcggcg cacatccgag
     3361 aggttctgct gcgaaagctc cagatcggcg cgccgcgcgt cccgcgttcc gtagcgcacg
     3421 ccgccgtcgt agatttgcca cgtcagcacg gccccaatcg tccacgacac atgctctcgg
     3481 ttttccgagc gcagcggggt actcgcgtaa ctcagcgtag agacgccgtc caaggtggga
     3541 atgaaagcgt agtccaccga cttgatgttg cgttcggcaa catgggtggc cgcactggcg
     3601 gcgcgcacgt caggccgcag ctcgacgttg gcttctcgtt ggcaaacacc ggcggcttgc
     3661 tcggcgaggt tgtcgagtcg aatgtccggg gtgatgccgt agggctcgct cgagctcagc
     3721 gcgttaccca gctcttcccg ggaacgcagc aggttttcgg ttgccgcaac gacttgagcc
     3781 cgcgagtcag acacctcttg ttcgatccgc agcacgtcga tgcgactggc cgcaccgagg
     3841 gcggctcgcc tgcggctcaa atccagcgtg gtcagggcac tgctcaacgc aacccggctc
     3901 acctcggcga ggcgctcctg ggtgactacg ttcacgatcg ttgcggctac gcgcgcgatt
     3961 tcccggcgct ccacgtcttt ttggtcgagc ttcgaattct cgatcacgtc tcgcgcggtg
     4021 gcctggtcgt accaagctcg cggcgcgagc acgggcacgc gcaagttcag gcctgcgacg
     4081 taatagcccg aggggttcgg gatcttgccg caaacggtcg ccgcctcggt cgtaccggga
     4141 ttgatcgtag tgcagccctc gccggtcaac aggtgctgcg tcgcggtccc gtttgcggtc
     4201 agggtcggta gagcgggagc gagcgcaagg cggcgttgag cggtagcttg ctgaatccgc
     4261 gcgcgcgagc tcttcagcgc cgtcgagttc tgacgcagga tcgtgagagc ctgttgccac
     4321 gtcgaaagcg tctgctgggc ggcgggtggt ggcaccagca tcggatcctc gagcttgggc
     4381 agcgttggct cagctcccgc ggctggtgcg gcagactcgg acgtggcttg cccggcgtta
     4441 ggcgcgggat tcgcagcggg cttgcccgct tgaccggcgg gcggcatcgg cgctgtgttg
     4501 acctgcccga gggcagatcg ggagatgcaa ttcagtacta gtaagcccgg tactaggatg
     4561 ctcgcgaaac ggcggcccgt agccatggcg ccggacatag cacgcgaccg cggcagagcc
     4621 tatcgggcct tcggggctgg tgagacgtac gctcggttca agtagcggtt ggcctcacgg
     4681 ggtctgctgc cgccgagcag ccggctgcta aggccacgcc ggggcacgcc ggtttcgaat
     4741 gcggagcgcg actactcgag gggccatgac tatttggccc ggtgggactt cacgcggcgt
     4801 tgcgtgcgcc acgcctgcaa ggttgcctgc gcgcgcttgg aatccccctc gaacactcgc
     4861 acgaggtgcc gcagtgcctc cgcttcgcgc cctgcgccga gcagcgcgtg cgcaagagag
     4921 ctggcgatct cgggctcctc gggcatgagc tgatgcgcca acttcagatg cggcaaggcc
     4981 gagagcggcc ggttgaaagc aacatccagc acgtggccga gattgtggcg gtgctccgca
     5041 gaatctggcg cgacttcgat ggcgcggcgg aaggcttcgc gagccgaacg aaaattcccc
     5101 agcaacatgt gggcgatccc cagcacgccg agcgcgcggt catcctcgcc aacgcgccac
     5161 tctccctcgg cacgcagcgc gtcgcgcgcc aatcgagccg cgagccacgg ctcactctcg
     5221 accaacacgg cggcgagctc caagcgtgca aaccgccgtt ctgctgagcc ttccggagcc
     5281 gccgccacca aacgtcgcaa catggccttg agctctcgtg ccggcagccg gtgagctttg
     5341 gcccacttca cgtctccgac cattcgttcg agctcgagcg gcattctgaa tgccctattt
     5401 ttgccgagaa tcggcgattt tcaaacgggc tcttcgtttt cttcttcttt ttgccccgag
     5461 cgctccgcgc caccccctcg ggagagctcg gccttgacag gacgccctgc gatctgcgcc
     5521 ccctcgaaag caccgagcac gctctccaga tcggcgcgct ccacggcaat gaaggcgttc
     5581 cgatgtcgca cgcggatgta gccaatggcg tgcgctggca cacctcgtgc ttcgaacaga
     5641 cgctcgtaat cgctcgcctt tacgccttgg cggcgtccca ggctcgcgta taactcgacc
     5701 caatcgccgg gtcgcgcgag ggccagcgga tcggtgggtg ggttggattg cgaagaagaa
     5761 gaagtcgcct cgacctcgtc cgagtcgagt ttcggggcga actcgggctg ggccggctcc
     5821 tgccgaagct ggccgcggcg catcgtctcg ggctcatcag aaatctcgga cagatccgtt
     5881 tcgggcgagg gtcgaggcga gggcgccacc tccgtgagct cggggagatc cgagaccgtg
     5941 tagcggggcg ttcccgagct caggttcgtg gtgcgctcgc ggcgcttcgc gccacgccca
     6001 tcgaacccac gccgctcttc atatggacgg cgttccgatc gatttggctc gtttcgtggg
     6061 gctctctctg gccgcgcggc acgctcatca tgcgccacgc gatcatcacg cgctggggca
     6121 gcgcgctcgt cgccgagttc gacggcttcg cggctttccc ggccttcgtt ggttcgccga
     6181 gcggcgaggc tggtgtcgcc gctttcgctc gcattgctgc gggattcggg ctcaccgggt
     6241 tcgccggctc ggcggtttct tctctcgaca tcgcggctct cgaagccatc acgccgatcc
     6301 gcgcggcgtt cgcttctgcg cgaaagcccc gtccgctcgt cgaggttctc tcgagcgggg
     6361 cgtgcttccg tcgcgcgcgc gagtccctcg ggggtgcgac gctcgccggc gtattcagac
     6421 gggccgccga gctcttcttc ctcagccgga gttctgtcgc cctgggcacc cttcccgatc
     6481 agcctatcgt ccgcggagct ttcgcgctct aggttgtcgc gtcgccgacg ttcgagaccg
     6541 tttcgactgc taccgctgcg ctctacggta gcgctgcgct ctacggtggc gctgcgctct
     6601 gcggtggcgc tgcgctctgc gattttaccg cgcccgcccg atggctcgcg cggacgagaa
     6661 gcttgactgg ttggtgtagc attaccggca gcgacgggaa ctacggcggc gcgtcgagct
     6721 gcggcggcag cctctggtgc ttcaggtagg gcgccgagat ggtcccgcag cagaccggcg
     6781 accacgactt cggcttgctc atgggtcagt agacgtcggg cgagcgccaa gtcgtcgtct
     6841 cgcacgcgcc gcttggcgaa cccctccacg aaccaacgca ccagatccgc ctcggctcgc
     6901 gttttcacct cgcgagccgt cggcagctgc cgctcgatgg gcttcagttt gtacgtcaac
     6961 cgcaacaggt acagattgcc gatatcgctc ggctcaatca tcgagatcgc ggtacccgtg
     7021 cgccctgcgc gcccggtgcg gcccgtgcga tgcacgtagt tctcggcgga ctcgggaaaa
     7081 tcgaaattga tgacgtgcgt taagtgggaa atgtcgatcc cgcgcgaagc aacgtccgtg
     7141 cagaccaaga atttcagacg tccctcgcgg gtggccgcca tcaccttctc gcgatctggc
     7201 tgcggcaggt ccgcgtttag ccagtccgcc gcgtagcctt gctgcacgag agcggctgaa
     7261 acccgcttcg tctcgtcgcg cgtgttgcaa aacacgatcg cgctctcggg gttctcggtt
     7321 tcgatcacct gcacgagctc ggccagtttg tcgctccggc tcagatacac gaaatgccga
     7381 atctccagcg cgccgatatg atcgccgctc agcgtgatga actccggttc gcgcaggcgc
     7441 gtctccgcca tgcggcggat gtcggggggc aaggtcgcgc tgaacaacaa tgtctggcgc
     7501 gactcgggta ggtgcgaaaa gatctcggtg atttgcggca aaaagcccat cgagagcatc
     7561 tcgtcgctct cgtccaaaac caccgcgcgg atcgacttgg ggttcagagt gccgcgttcg
     7621 aggtgatcga gcacgcgccc cggcgtgccg gcgacaatct gcgctccatc gcgaatgcga
     7681 tcgatctgcg cctgcatcgg cgcgccgccg tacactgcga cgatccgagt cgcgcggtag
     7741 cgcgcgaggg cctcgagttc acgagtgacc tgcagggcga gctcgcgcgt cggacacaac
     7801 accagtgcct gaacctctgc ttccttgcgg cgcacgatcg cgtcgatgag cggcatgccg
     7861 aaagcagctg tttttccggt tccagtgcga gcctgcaccg ccaaatcgcg accgcggctg
     7921 gcgggctcga ataccgcacg ttgcacgggc gtcggatgca cgtagccgag ttcgtccacc
     7981 gcctttcgca agtcttggga aagaggaaga acatcgaacg tcggaagtgc ggatgtatcg
     8041 ggttctgaag acattgtact aggggcgggg agagatcgag aattcgcgac gcagcgcctc
     8101 ggcccgcctg cgttgcttgg ctagcgccac cgcctcgtag cgaaccgcat gctcttctgc
     8161 atagcgaagc gcatccagat cggaaagagc ggcgagagct cgggagtcac cccgacagac
     8221 ttcgttcaaa gcctgtcggc cctgccattc tggcagaagg ctgcggcgaa agcggtcgag
     8281 cgcggcgcgc tcgccgtgag attcctgagc cgcgtccgtg cgcgcacgcc gcaccgcggc
     8341 ttcgagttcg aggatcgaac cccgacacgg accgcgagtg ggcacggttt cgggcgagaa
     8401 aacctgccga agaacttgtg aagtccagat cgccgtggcg ccaaaaactg gcacggcaaa
     8461 ggccaaaatg cccagtcgac ggcccacgcg agcaatccga gcgcgcgcgc cggcgttgtg
     8521 catggcctgc gcatttgcgc acattcagcg gcgtcgtcaa atcggatcag tcaaatctaa
     8581 ggtcccccgg gtcatccggg ggttccggaa aaccgaccga gcggccgccg gatgttctcc
     8641 gccggctccc tggggtcggt tgttcgttct ggcgttgacc tctgagtcag acgcctccat
     8701 agttctttca caaaactttg aaacctcaga aactcgaagg gacttctccc ttgaccctgc
     8761 aggcagctac atctgttctt gccgatactg cacaagccac cctcgcgggg ccccgcgcga
     8821 tggaccggct cgaagccatc caaagcttgc tgtcggatga cctgaggtgg atcgaggaga
     8881 gcctacgtca ggtgtgcgat gacggccctg ctcctgccgt ccaggcggcc cagcaccttg
     8941 tatctcgagg cggtaagcgc gttcggccgc tctcgatgct gctcagcgcg gcctgttttc
     9001 gtcccccgag cgatgttgtg cgtgaacttg cggtcgtgtc ggagctcgtc cactccgcga
     9061 cgctgctgca cgacgacgtc gtggacgaag gagacgagcg acgcggcgcg atggcggcgc
     9121 gactcgtgtg ggggaatgcc gtcagcgtgt tgggcggcga tttgctcctc gttcatgccc
     9181 tcgagcgcac cgcgcgtctg gcgcccgagc tgatgggcga tttgatcgcc accctgcgcc
     9241 gcttggtcga cggtgagatc gtgcagctgc ggggacgcaa cgagctcgac gtgtccgaga
     9301 ccacctacga gcgcatttta cgcgacaaga ccgcgtcgct atttgcttgg tccacgcgca
     9361 cgggagcgca cctgagcggc gccagccgta gcgatcagga ccgcctgtcg gtcttcgggg
     9421 agcgtctcgg gctggcgttt caactcgtcg acgatgtgct cgactattcg ggcgacgcca
     9481 gcggcaagag tctgctcacc gatttgcgtg aaggtaagct cactttgccg ctggtgctca
     9541 cggtcgcaaa ggtgccggag ctgattggtc cgctgaagcg gatctacgcc ggggatcgcg
     9601 agcccgtgac gctggtgagt cgcgccgttc aagagtgtgg agcgttggac gaggtgcggc
     9661 gccgcgcgac tcatcatacg catgaagccg tgcgttcact cgaggcggtg caaccaagcc
     9721 cagcccgtcg ggtgctagaa caggtcgcga gcgagttagc gcgccgcgca gcctgagccg
     9781 tcacgacgcg caaggccacc tcagaaaaca gaagaccccg caggaccgac tcattcggtg
     9841 ccggcggggt ctctggtttt gggacgcagg aatcgagccc agcttctgcg ctcagttcaa
     9901 cgtgagattg cgatcgccac tggtctcctg ctcgaggaac gtcgcggtga tttcccgctc
     9961 cagcgctagt tgcagctcgc tctccacctg ctcgacggag cctgccaagc gttccacgtt
    10021 gcgctgatag acgaagacgc gagcattcat gctgttgccc gcggccccga cgccatcgat
    10081 tagcaaaaga gcgcgcgggt ctacgccatc caccacgacc tccacgccgg gcacgtcgca
    10141 cacgtagatc tcttcttcgc gaagatacac gcgcagtctc tcatccaacg cctggaaagc
    10201 ccgcttggcc gtgagcgcaa aggtctccga cgacaaagtc caaggcggag cgggcgtctc
    10261 caaatcgagc tcgcgcgagc gtaggaaagc ctccgttgcg gcgtgggtat caccgcgctc
    10321 gtctcgcacc aagcccaagt agtaataggc ctcggcgttt cgcgcattct gctttacggc
    10381 ttcgtcgatc aacggctccg cgtgatcgat gtcgccgatc tcgtagtagg cgcgaccgac
    10441 caagaacacg tgattcgggt tttcgaacgg tccgggtggg aattggccgc agaccagcgc
    10501 cgcctggtcc aattcgccct tgccgagtag agcatcgaat ttcaacaaca gagcgtcgac
    10561 gatctcgtcg tccgtttccg ccagatcgag cgcctgatca cacatgttga tcgcgtcgtc
    10621 gaactcaccg agcggatgta tgtatacctc tgccgcgttc agcatggctt cgagataagt
    10681 gtcatccaat gcgaccgcct ggcgatagtg gtcgatggct tcctcgtatt cgcccttcaa
    10741 tgccgcgaca tagccgagca ggttgtaggc ttcaggtgac tggctgtcga tttccaacgc
    10801 tcgacgcgca gacacttccg ctcctcgagg gtcaccccgc tgaacgaggt cccaaccgcg
    10861 gtcgagatgt gccgagaact gatccatggc agccgaagtt gatggtccgg acacgcgcct
    10921 acgtcaacta catccggctg gaacgcgcat cagttaagtg gtcggccgaa tcctgtgggc
    10981 gatgaggggc atgccgggct gtgagcacgg cgtgtcggca gggcggcccg gccctccacc
    11041 cgcgccgcga tccgcatcca ccgcccgctc ggcggcggct gagcttgccg gcgt
//
LOCUS       DASTKU010000558         3814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_555032, whole genome shotgun sequence.
ACCESSION   DASTKU010000558 DASTKU010000000
VERSION     DASTKU010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555032"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFQ61_19335"
     CDS             <1..237
                     /locus_tag="VFQ61_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HET9956663.1"
                     /translation="CHLHGINPQTYLEEILRLAPHWPATQLLQLSPKYWPQTRAALTA
                     AQLKCLRSPWDKSAAPAQPAPAAEASEPSAARAA"
     gene            complement(507..899)
                     /locus_tag="VFQ61_19340"
     CDS             complement(507..899)
                     /locus_tag="VFQ61_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HET9956664.1"
                     /translation="MKLLLDMNLSPEWTRFLEQEGFEAVHWASVGSPTASDAEIMEWA
                     REREYVVFTHDMDFSALIAVTGAAGPSVLQVRTQDVLPAAIGRDVVRVLTMRTRAFEA
                     GAIVTIDKLKSRVRVLPIGRSSEGSEPV"
     gene            complement(896..1219)
                     /locus_tag="VFQ61_19345"
     CDS             complement(896..1219)
                     /locus_tag="VFQ61_19345"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET9956665.1"
                     /translation="MAESCGCTPIGPFPAPCGNRAVGKSQISGYNLQMQLLNRITHDP
                     TVMGGRPCIRGMRVTVGTIMGLLASGRTQQEILSAYPYLEAEDVQAALAYAAWRTQEV
                     ELPLT"
     gene            complement(1535..1996)
                     /locus_tag="VFQ61_19350"
     CDS             complement(1535..1996)
                     /locus_tag="VFQ61_19350"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET9956666.1"
                     /translation="MSEVEPVLLDTDTLSELSRGNATVAARAQTYLRAFGRFTISSVT
                     VFERLRGYRQAIRAGKPFHRQLHAFEALVQNSVVLPFDENAAAVSSTIWATSSRGQRQ
                     KLGDILIAGIAVARQIPLATRNQRDFEVLSKNAEVSLHLVDWTVGAKRVSR"
     gene            complement(2391..2792)
                     /locus_tag="VFQ61_19355"
     CDS             complement(2391..2792)
                     /locus_tag="VFQ61_19355"
                     /inference="COORDINATES: protein motif:HMM:NF025677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HET9956667.1"
                     /translation="MGHCPPPAGRPRFDIADIVREHRAALERERRLSPTEKRVLTAMA
                     RCRTAALGGHVEVCRTCGHQQPAYSSCRNRHCPKCQCLAQEKWIAARSERVLPVRHFH
                     VVFTVPSELRALCRRAPRALFEALFHTASGV"
     gene            complement(2799..3317)
                     /locus_tag="VFQ61_19360"
     CDS             complement(2799..3317)
                     /locus_tag="VFQ61_19360"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9956668.1"
                     /translation="MGKLLDALESPKYRVFFTTLYATGLRLREASHLEVGDIDAARGV
                     IHVRHAKGQRERLVMLSPRLLAILRAYWKQERPARPYLFPTKAGKPLDPDFARRVLHK
                     AAKAAGLSKVTPHVLRHSFATHLLDSGTELRVIQVLLGHDSIRTTTRYARVSTAVLAK
                     TKSPLERMRKTG"
     gene            complement(3232..3633)
                     /locus_tag="VFQ61_19365"
     CDS             complement(3232..3633)
                     /locus_tag="VFQ61_19365"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HET9956669.1"
                     /translation="MTMLKDRMMQDLKLAGHALKTQKTYVECIGHFAQFCWSPEQADQ
                     TVVRAWVEKLQKSQVGVAAAPAALRGAEVPVRQDPCTPGAGGASVVAKGSEAAAEGVD
                     RGAGGQAARCAGVAEVPGLLHHAVRDWFAVA"
BASE COUNT          698 a         1317 c         1093 g          706 t
ORIGIN      
        1 tgccatctgc acggcatcaa cccgcagaca tatctcgaag agatcttgcg cttggccccg
       61 cactggccgg caacgcagct gctccagctc tcacccaaat actggccaca gacacgcgct
      121 gccctcaccg ccgcgcagct caagtgcctg cgttccccct gggacaagtc tgccgcgcca
      181 gcccagcccg ctcctgctgc cgaggcgagc gagccctcgg cagcccgcgc ggcgtagccg
      241 cccgccgggc agtcgtgcgc gccctagcag cgggcgggcg agcccgagac ggtcgcggct
      301 ccggacacac cccgccccgc gcgcgagctc accacaggtc gcgcccgcag gaccaggacg
      361 ccctttgccg aacgggtact taaactgcgg cccatcagtc tccgctcaac ctctcaacgc
      421 gggacgaccg ctgccttggc gcgaagcgct caaggcagcg atgcatactc ttccaagtcg
      481 gaagcccgtc agcttcaatg tccgagttag acgggttctg aaccttccga gcttcgtccg
      541 attggcagca cgcgaacccg agacttgagc ttgtctatcg taacgatggc gcccgcttca
      601 aatgctctag ttcgcatagt cagaacgcgc acgacgtcgc gcccgatcgc cgccggcagg
      661 acatcttgcg tgcgaacctg tagcacactg ggccccgccg caccggtcac cgcaattagt
      721 gcggagaagt ccatgtcgtg cgtgaacacg acgtactctc gctcccgcgc ccactccatg
      781 atctctgcat cgctcgcagt tggcgagccc acagaagccc agtgtacggc ttcgaatcct
      841 tcctgctcca agaacctggt ccattcgggc gacaggttca tgtcgagaag tagcttcatg
      901 tcagtggcag ttccacctct tgcgtccgcc aagcggcata ggcaagggcc gcttggacgt
      961 cctccgcctc gaggtacggg tatgcagaga ggatttcctg ctgcgttctg cccgacgcaa
     1021 gcagacccat gatggtgccg accgtcacgc gcatccctcg aatgcaggga cgaccaccca
     1081 tgacagtcgg gtcgtgcgtt atccggttca acagctgcat ttgcagatta tagccagaaa
     1141 tttggctctt gcccaccgct cgattgccgc agggcgcggg aaaaggccct atcggcgtgc
     1201 agccgcacga ttcagccata tggcagaatg tgatgagggc gcgcccctga ctgtgctgga
     1261 gtcgcgaggt cttcgaaatg ccgcttgcag ccgccacgct ccggctcaac tgacgcaagc
     1321 tgacggcgca tcgcccgcac agtcacgttc gatggaacat cttcgctccg tctaacggat
     1381 ggcataagct gcgggcccat caagctcggc tcaacgtgtg aggtcggaac gaccgctgcc
     1441 ttggcgcgaa gcgctcaagg cagcgaggca gggctttcca ggtctgttgc ccgtcagctt
     1501 caatggccga gttatgcgcc gtccgtttgc ttggtcatcg actcacccgc ttggctccga
     1561 cagtccaatc cacgagatgc agactcacct cggcgttctt gctcagcact tcgaagtcac
     1621 gttgattccg agttgccaac ggaatttgcc gggcgactgc aatgcccgca atcagaatgt
     1681 cccccagttt ttggcgctgc ccgcgggagc tggtcgccca tatggtgctc gagactgcgg
     1741 cagcattttc atcaaacggg agcacaacgg agttctgcac gagtgcctcg aacgcatgca
     1801 gctgccggtg aaagggcttt cctgcgcgaa tcgcttgccg atagccgcga agccgctcga
     1861 agaccgtcac tgagctgatt gtaaatcgtc caaacgctct caggtacgtc tgtgcacgag
     1921 ccgcaacagt agcgttccct cggctcagtt ccgacaaggt gtcggtatcc aacaggaccg
     1981 gctcgacctc gctcaccagt cagaccctag cgatcagtgg ccttcaactt gcgttcaatc
     2041 aaccgagcgt cagccggtga aatgtccgcc atgctggctg ttgccagtgc caggatttcc
     2101 gcgggactcg gtgcgatatc cacgtcttct ccatagtgac gtcggagcaa cgcgatcacc
     2161 tggccctcga aactcctgcc gcatcgttct gcatcagttg cgatttgttg cctgagcccc
     2221 gcagagaagc gaatggttcg tgcaaccgtg gagtcggatg ctgtcgcgga tcgtgccaca
     2281 ccgaaaaagt accatattgg tttgtgtagc gcaagcaccc ctccgaaccc cagggactcc
     2341 acatgtccct gagtgtcgtt agccacgcgc caccagatca tcatcgagtg ctagacgcca
     2401 ctggcggtgt gaaagagggc ctcgaagagt gcccggggag ccctccggca cagcgcgcgc
     2461 aactcgctcg gcaccgtgaa gaccacgtga aagtgccgca cgggcaacac ccgctcggag
     2521 cgcgcagcaa tccacttctc ctgcgccaag cactggcact ttggacagtg ccggttgcgg
     2581 caggagctgt acgcgggctg ctgatgtccg cacgtgcggc acacttcgac atgaccgccg
     2641 agcgcagcgg tgcggcaccg tgccatggcg gtcagcacac gcttctcggt cggactcaac
     2701 cggcgctccc gttcgagcgc ggctcggtgc tctcggacga tgtcggcgat gtcgaagcgg
     2761 ggccgcccgg cgggtggcgg gcaatggccc acgcgatctc agccggtctt gcgcatgcgc
     2821 tcgagcggac tcttcgtctt cgccagcacc gccgtgctca cccgcgcgta cctcgtcgtc
     2881 gtccgaatgc tgtcgtgacc gagcagcacc tggatcacgc gaagctccgt accactgtcg
     2941 agcagatggg tcgcaaacga atgccgcagc acgtgcggcg tcaccttgct cagccccgcc
     3001 gccttcgctg ccttgtgcaa cacgcgccgc gcaaagtccg gatccaatgg tttgcctgcc
     3061 ttggtgggaa acagatacgg ccgcgccggt cgttcctgct tccagtacgc tcgcaggatc
     3121 gccagcagcc gcggactgag catcaccagc cgctcccgct gccccttcgc atgtcgcacg
     3181 tgaatcactc cccgtgcggc atcaatgtcc cccacctcga gatggctcgc ctcacgcaac
     3241 cgcaaaccag tcgcgtacag cgtggtgaag aagacccggt acttcggcga ctccagcgca
     3301 tcgagcagct tgcccacctg ctcctcggtc aacaccttcg gcagccgctt cggatccttt
     3361 ggccacgaca gaagcgccac ctgctccggg cgtgcaaggg tcttgccgta caggaacttc
     3421 agcgccgcga agtgctgccg gagccgctgc gacccccacc tggctcttct gcagcttctc
     3481 cacccaggcg cgcaccaccg tctggtccgc ttgctccggc gaccagcaga actgcgcgaa
     3541 atgaccgata cattccacgt acgtcttctg cgtcttcagg gcatggccag ccagcttcaa
     3601 atcctgcatc attcggtcct tcagcatcgt catgatgggt ctccttggtt ggcggtgcgt
     3661 caacaccgcc cgccaaggag gcccccgctg aagtttcggc cgagagcgcg tctcccaatc
     3721 ctcgcgcaat cgctattgct gctcgacttc gactacgcgc ccgagcctcg gcgcgcagca
     3781 cccccgccgc ggagcggctt cgttcaacga ccgg
//
LOCUS       DASTKU010000559         4210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_555036, whole genome shotgun sequence.
ACCESSION   DASTKU010000559 DASTKU010000000
VERSION     DASTKU010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555036"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFQ61_19370"
     CDS             <1..343
                     /locus_tag="VFQ61_19370"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HET9956670.1"
                     /translation="PANTILVYENFVKGLKGAGPADRAEVARGEALVKQYAAVLDAHL
                     AGKQWITQERLTLADFSVAASFALATPGRLPIEDFTNLKSWLGRVQELDAWKRTAPPA
                     PPTPRRENSAA"
     gene            436..1800
                     /locus_tag="VFQ61_19375"
     CDS             436..1800
                     /locus_tag="VFQ61_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HET9956671.1"
                     /translation="MKSRSSRPVSSSWLVLGAVLVACSAATEGDPDSGSGGGHYTGGV
                     SGTSSGGTKSSSGGAPSSGGTSQTASGGASSGGANASGGKEPSGGSSQMDRGGSGGSP
                     ASGGSRTSGGASSGGSYSMGGSSGGRSGSGGAGSGGSSNSMGGAKSSGGSAGATASGG
                     GSSGPPKKFVGNITTRDSVDTNGMTFSKYWDQITPENSGKWGSVQSTPSSAFNWKSLD
                     AVYDYAQKNGILFKQHTFVWGSQQPQGTPTLAQVENWIKTFCQRYPNTKLIDVVNEPP
                     PHTTPSYTANLGAGESGTYGWITKAFKLARQYCPNAVLILNDYNNIEYGDQENHFIDI
                     VNQIKAAGAPIDAVGCQSHALKGLSASTVQANINTMATKTGLPVYITEYDIGEADDAK
                     QLSIYQAQFSIFLNTAAVHGVTVWGWINGSTWVQNTGLMNGTKPRSAMTWLMGQLGRP
                     VPPN"
     gene            complement(1915..3204)
                     /locus_tag="VFQ61_19380"
     CDS             complement(1915..3204)
                     /locus_tag="VFQ61_19380"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET9956672.1"
                     /translation="MRLVRLFAPALLVALCPKPALAVASAELYNTQAYVYGRFEARIR
                     YAPGDGVISSFFLWKEGSEKDGAYWNELDFEKLNSNCRMQTNAIFGNPESGHEQSHTD
                     LGEICAGYHDYAFEWTPSYISWVVDGREIRREAGSIATAFSQNATAGMTFHFNVWPGN
                     ANFGGNFDPAILPTRQYISWVRYSSFKNGSFQKEWQEDFQGTGVPSGWAVGSWASPLN
                     LSTHNASNVTFASGIAVLSLTSDNGTGFSGDPPKDMGGAGGMTNGSGGTTAASSGGAA
                     GAGRGGQSAAGGNGNGSGGDRTSGGANSGGSQGLGGIASASGGTSQSSGGASSSGGAV
                     NSGGSTSASGGSSSGGASSGGSQASGGSAVAGGASNSGGALNAAGGSGAASGAPSNKD
                     AGCSCSVAPRDLDAAPFAAVAVAVASACVRRRRRPAR"
     gene            complement(3201..4085)
                     /locus_tag="VFQ61_19385"
     CDS             complement(3201..4085)
                     /locus_tag="VFQ61_19385"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET9956673.1"
                     /translation="MLSSTAAHAAVSSAELYTSQSYTYGRVEARARFAAGDGVVSSFF
                     LWKNGSEMTGTFWNELDFEKVGADCHLQTNALFGLPLVDHGRNEGAALNLCNAFHTFA
                     YEWTPDYIAWFIDGTEVRREAGDTAKAYADNATAGMQIRFNIWPGDASFGGNFSPSIL
                     PVHEYIDWVQYSTYTDGAFNVAWREDFDGTSAPTGWLVGSWASPKNLSTHSAQNVTFV
                     DGHAVLSLTTDDAVGFKGKIPLDGQPDKNPSDSDDSGGCSVRNPHGASGRSLLFLSGL
                     AFAAVASVRRRARRRGRP"
BASE COUNT          801 a         1329 c         1356 g          724 t
ORIGIN      
        1 gcccgcgaac acgatcttgg tttacgagaa cttcgtaaag ggactgaaag gtgcgggtcc
       61 ggcggatcgc gcggaggtgg cgcggggcga ggcgctcgtg aagcagtacg cggcggtgtt
      121 ggacgcgcac ctcgccggga agcaatggat cacgcaggag cgactgacgc tcgccgattt
      181 ttcggtggct gcttcgttcg cgttggcgac gcccggtcgg ctaccgatcg aagactttac
      241 gaacctcaag tcgtggctgg gtcgcgttca agagctcgac gcgtggaagc gaacggcgcc
      301 gcccgcaccg ccgacaccgc gacgagaaaa ctctgcagcg tgagcaacgt ctcactctga
      361 taagtaaagg atgttcgcca aaacatcgtc gtaactacgt cgacggaaat atcggtggtg
      421 caccactgct ggaacatgaa gagtcgctct tcgcgtccag ttagtagctc gtggctcgtc
      481 ttgggtgccg tcctcgtcgc ttgcagcgcc gcaacggaag gcgatccaga ctctggttcc
      541 ggcggtggac actataccgg aggggttagt ggaaccagct cagggggcac caaatcgagc
      601 agcggtggtg cgccatcgag cggaggcacg tcgcaaactg cgagcggtgg cgcttcgagc
      661 gggggagcca atgccagcgg tggaaaagag ccttctggcg gctcttcgca gatggatcgc
      721 ggcggctccg gcggaagtcc cgcgagcggc ggctcacgta cgagtggcgg cgccagcagt
      781 ggtggtagct attcgatggg cgggagctcc ggtgggcgct ctggctccgg tggcgcgggc
      841 tcaggcggaa gctccaattc gatgggcggg gccaagagct ccggcggaag cgcgggcgca
      901 accgcgagcg gaggtgggag ttccgggcca ccgaagaagt tcgtcggcaa catcacgacg
      961 cgcgactcgg tcgatacgaa cgggatgact ttctccaagt attgggacca gatcactccc
     1021 gaaaattcgg gcaagtgggg ttccgttcag tcgacgccca gcagcgcgtt caactggaag
     1081 tcactcgacg ccgtctacga ctacgcccag aaaaacggga tccttttcaa gcagcacacc
     1141 ttcgtttggg gcagccagca accgcagggc acgccgacgc tcgcgcaagt ggagaactgg
     1201 atcaagacgt tctgccagcg gtatcccaac accaagctca tcgatgtggt caatgaaccc
     1261 ccgccacaca ccacgccgtc ttacaccgcc aacttgggtg cgggcgagag cggcacctac
     1321 ggctggatca ccaaggcgtt caagctcgcg aggcaatact gtcccaacgc ggttctcatt
     1381 ttgaacgact acaacaacat cgagtacggc gaccaagaga accacttcat cgacatcgtg
     1441 aaccagatca aagcggcggg agctcccatc gatgcggtgg gttgccaatc gcatgcgttg
     1501 aagggcctgt ccgcctcgac ggtgcaagcc aacatcaaca ccatggcaac caagactggc
     1561 cttccggtgt acatcaccga atacgacatc ggtgaggccg acgacgccaa acagctgtcg
     1621 atctaccagg cacagttctc gatcttcttg aacaccgccg ccgtgcatgg tgtgacggtg
     1681 tggggctgga tcaacggttc gacgtgggtc cagaacaccg gcttgatgaa cggaacgaag
     1741 ccacgctccg cgatgacgtg gctgatgggg caactcggtc gccccgtgcc accgaactga
     1801 gtaagaaccg gcggtccgcg gcgcgcgctg attgctctac tcggcgcggg ccgatccttc
     1861 gctcagcgcg ccgggcgaca attcgctcag cgcgccgggc gacgaatcct tcgattagcg
     1921 cgccgggcga cgacgccggc gaacgcaggc tgaagcgacg gcgactgcga ccgccgcgaa
     1981 gggagccgca tcgagatcac gcggggcgac ggagcaacta cagcctgcgt ccttgttcga
     2041 gggggcgccg ctcgccgcgc ccgagccacc cgccgcgttg agggcgccac cgctgttgga
     2101 cgcgccgcca gcaaccgcgg atccgcccga agcttggctt ccgccagagc tcgcaccacc
     2161 ggagctgctg ccgccggatg cgctcgtcga gccacccgag ttcactgcgc cgcccgagct
     2221 cgatgctcct ccagaacttt ggctggtacc gcccgatgcg ctcgcgatgc cgccaagacc
     2281 ttgcgagcct ccagagtttg cgccgccgct agttcggtcg ccgccactgc cgttaccgtt
     2341 gccgccggcc gcactctgtc cgccgcgccc ggcccctgcg gcccctcccg acgaagctgc
     2401 ggtggtgccg cccgagccgt tcgtcatgcc gcctgccccg cccatgtcct tcggcggatc
     2461 accagaaaac ccagtgccgt tgtccgaggt gagcgagagc acggcaatgc cactcgcgaa
     2521 tgtcacgttc gaggcgttgt gcgtggagag gttgagcggc gatgcccaag agccaaccgc
     2581 ccagccactc ggcaccccgg taccctggaa gtcctcctgc cactcctttt ggaaagagcc
     2641 gttcttgaac gacgagtacc gcacccagct gatgtactgg cgcgtcggca aaatggcggg
     2701 atcgaagttg ccaccaaagt tcgcgttgcc gggccacacg ttgaagtgga aggtcatccc
     2761 ggccgtcgcg ttctggctga atgcggtcgc gatggagccc gcttcgcgac ggatctctcg
     2821 gccatccacg acccaggaga tgtacgaagg cgtccactcg aacgcgtagt cgtgatagcc
     2881 cgcgcagatc tccccgagat cggtgtgcga ttgctcgtgg ccggattcgg gattgccgaa
     2941 gatggcgttg gtttgcatcc ggcagttgga gttgagcttc tcgaagtcga gctcgttcca
     3001 atacgcgccg tctttctcgg agccctcctt ccaaaggaag aaggagctga tgacgccgtc
     3061 gccgggcgcg tagcgaatgc gcgcttcgaa tcgcccgtag acgtaggcct gcgtgttgta
     3121 gagctctgcg ctcgcgacag ccagcgccgg cttggggcag agcgcgacca gcaacgccgg
     3181 cgcgaagagt cggacgagcc tcacgggcgc ccccgccgcc ttgctcggcg gcgcaccgaa
     3241 gccaccgccg cgaacgcgag accggacaag aagagaaggc tacggccgct cgccccgtgc
     3301 gggttgcgca cgctgcaccc gcccgagtcg tccgaatccg aaggattctt atcgggctgt
     3361 ccgtcgaggg ggatcttacc cttgaagccg accgcgtcgt cggtagtgag cgacagcacc
     3421 gcgtgaccgt ctacgaaagt gacgttctga gcactgtggg tcgagaggtt cttcggcgag
     3481 gcccaggacc cgacgagcca ccccgtcggc gccgaagtgc cgtcaaagtc ttcgcgccag
     3541 gcgacgttga acgcgccgtc cgtgtacgtc gagtattgaa cccagtcgat atactcgtgg
     3601 accggtagga tcgatgggct gaaatttcca ccgaagctcg cgtcgcccgg ccagatgttg
     3661 aaccgaattt gcatgccggc ggttgcgtta tctgcgtatg ccttcgccgt gtcgccggct
     3721 tcacgccgca cctcggtacc gtcgatgaac cacgcgatgt agtcgggcgt ccattcgtaa
     3781 gcaaacgtat gaaacgcatt gcagagattg agggcggcgc cctcgttgcg cccgtgatcc
     3841 acgagcggga gaccgaagag ggcgttagtc tggaggtggc agtccgcccc caccttttcg
     3901 aaatcgagct cgttccagaa cgtgcccgtc atttcggagc cgttcttcca caggaagaag
     3961 gagctcacga caccgtcgcc agcggcgaac cgagcccggg cctccacccg tccgtaagtg
     4021 taggattggc tcgtgtagag ctcggcgctg ctcacggcgg cgtgcgcggc ggtcgaactc
     4081 aacatgcaga gaagaactcc cgagaggcac cagctgatct tcatcttcgt tcacccagtg
     4141 atatcggagc aggtggggtg gagtggttct ttcacttcgc attttgtgcc aaaactggcc
     4201 gcagggctcg
//
LOCUS       DASTKU010000560         6401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_555365, whole genome shotgun sequence.
ACCESSION   DASTKU010000560 DASTKU010000000
VERSION     DASTKU010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555365"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1831
                     /locus_tag="VFQ61_19390"
     CDS             <1..1831
                     /locus_tag="VFQ61_19390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9956674.1"
                     /translation="DPDLEELWHEARSQISLLATRSHVRRRVQLEDTYDRLLRTGERF
                     STNLELSSLSEALREELPVAQVRNAFVALYGPEGCGTLDPLFCLVDGESRAMLPEAYP
                     TAEIFPPQTFSTRRRTTWVLLPLTFEAERLGVVGFEYTTSVVAYSMFRDRISAALKTA
                     DMHQEIVIQTAAHERNRQERLATAERIKSLSLLAGGVAHDLNNALGPLAALPDVLVHE
                     IDRLADWDSPAKREIVADLGLIKSAAQRAAQTIKDLLTLGRQHQVKKETLDLNRVAAA
                     CASGALDRPDRIELVLELGPEPLWVNASDSHVSRAITNLLRNAAEAIPERGQITLKTW
                     GEYLGSVFSGYELVEPGEYAVLSVRDSGQGIAPEVLKRIFEPFFSSKKLRDGSGSGLG
                     LAIVHGVVKEHGGYINVMSRPGLGTEFTLYFPKAPSAPKALAEPAILPGKGRGKLLVL
                     DDDPMQLRTAKRILSHAGYDVVTADSGQRALALLRESDSSPEAAEPSGRGSASPFDLV
                     LLDMNLNEAEDGLEIFERMRSTYPNQRGILVSGHAPLERGELAQHRGLTWLSKPYTSD
                     ALLLAVQTVLLQVSPSYRPLWHPSDIEQRHLSFGPPESQSGVH"
     gene            complement(1853..2227)
                     /locus_tag="VFQ61_19395"
     CDS             complement(1853..2227)
                     /locus_tag="VFQ61_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956675.1"
                     /translation="MSSSTPRPHLTILEPAPAPSTPSPGMLAAALAGLMCISTACGSK
                     DDEKANTEATVTSSRIEPDMTLEKFSEECTVRGGRVETHAHCGGLNTCRGFSYDTETL
                     TLTEHTCKQLNTCAGYSCVLGS"
     gene            complement(2305..2916)
                     /locus_tag="VFQ61_19400"
     CDS             complement(2305..2916)
                     /locus_tag="VFQ61_19400"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9956676.1"
                     /translation="MQLYFSPTSPYARKVRCVLLEKQLPFEGIDVAATNRSPAEHNPL
                     GKVPTCILDDGLVLFDSRVIIETIEAMHPLPGLIPMEVHDRALVRRWEAIGDGICDVL
                     IPVVLDSRRAPELRDAAYAQKLEGKVRASLSYIEQALRGREFVHGERFTLADIAVIAA
                     TGYVKLRRPELLDGYPELDRYIAQQLQRPSLASTVPPNLPVRG"
     gene            complement(3048..4679)
                     /locus_tag="VFQ61_19405"
     CDS             complement(3048..4679)
                     /locus_tag="VFQ61_19405"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9956677.1"
                     /translation="MSHCSWIVSTLAVSAVVALSACSHSTSPNDTMSPSNSGGAAGHS
                     GGGSPAGGSLAGSMSTAGGAGGTAGALSGGAGFGAQAGANGGTLAFGGAAGSSAFAGA
                     SFGGGGSAGFAGAGSGGAAQVCPPACGPSYEAFFNNNEVATLKVYIEGSDLAGHAASD
                     WLALLKTKWKHCRPFDNYVRSRVVYEGSSGKFELSDVGIRLRGSREQPVRGFKLDFQK
                     LLGTATGDARRRFADLNRVNILSIEDDDSHMVQCLAYKALRDFGVQAPRCNHLRVYVN
                     DQFYGLLESVEEADHGRFLRHHWGTSEGLLVEASPSESDCGFSDGKANLEYEGDTSNT
                     TYAAAYKAERGTQADMEKFLFPMFKCADKDTTPDDAQFKTCISEWLDVQEWLKVIAFE
                     SLTPELESFIGYKRNFYLYFHPDATSPRGGRFLVYPWDLDTAFQRQKCYPSNCDPFKS
                     VSSFLGSRGIRPPLVTRLTTVFKTEYCTAMKAYLNDVFKTSLIDDMAKVVEPAMMGDP
                     HASASVWQAEVSKLRSHIDSQKSAMGALITAACGG"
     gene            complement(5025..5444)
                     /locus_tag="VFQ61_19410"
     CDS             complement(5025..5444)
                     /locus_tag="VFQ61_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956678.1"
                     /translation="MMKKTCAVLAVAFSVFGLVGCGGDDDDSPAKGGSGGSGGTSSSS
                     GGSSSTGGSTALPAWEKDCHDYCAKRVACETDLSLTDCYDYKCQFQGVPDNKLASCQA
                     ASQVVWACEMKQPNVCDSAAVETACSSENEDVATACN"
     gene            complement(5627..>6401)
                     /locus_tag="VFQ61_19415"
     CDS             complement(5627..>6401)
                     /locus_tag="VFQ61_19415"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HET9956679.1"
                     /translation="SMALTPIAFLILERSILPRATSSSPRRDDDEITDGHQPVVIAGF
                     GRFGQIVGRMLRASGIGTTVLDLDPGMVSLLRRLGHAVYYGDASRPELLYAAGCEHAK
                     LFVIAIDDMEQALELAKMLRKHFPKLRILSRARNREHYYALRRAGVDEVFRETFGSAF
                     ELGVAAFRSLGYRAHTAQRLADVWRRHEEGALRDLESLYDLDQDSYFAAARRALEQAE
                     RLMRAEDPARIKREYEAGWDNETLRQEATSGQQGPETRG"
BASE COUNT         1203 a         2064 c         1949 g         1185 t
ORIGIN      
        1 ggatccggat ctcgaggagc tgtggcacga ggcgcgcagt caaatctcgc tgctcgcaac
       61 gcgctcacac gtgcggcgtc gcgtgcagct cgaagacacc tacgatcgcc tattgcgcac
      121 tggcgaacga ttctcgacca atctcgagct ctcgtccttg agcgaggcgt tgcgggaaga
      181 gctgccggtg gcccaggtac gcaacgcttt cgtggcgctc tatggccccg aaggttgcgg
      241 cacgctcgat ccgctgtttt gcctggtcga cggcgaatcg cgggccatgc tacccgaggc
      301 gtaccccacc gcggaaatct ttcccccgca aacgttctcc acccgccgcc gcaccacctg
      361 ggtgttgctg ccgctgacgt tcgaggccga gaggctgggc gtcgtcggtt tcgagtacac
      421 caccagcgtg gttgcgtact cgatgtttcg cgatcgcatc tccgccgccc tgaaaacggc
      481 ggacatgcac caggagatcg tgattcagac cgccgctcac gagcgcaatc ggcaagagcg
      541 cttggcgacc gccgagcgca tcaaatcctt gagcctgctc gcgggcggag tggcccacga
      601 cctgaacaat gcgctggggc cgctggccgc gcttccggac gtgctcgttc acgagattga
      661 tcgcttggcg gactgggatt ctccggccaa gcgcgaaatc gtcgccgacc tcgggctgat
      721 caaatctgcg gcgcaacgcg cggctcagac gatcaaggat ctattgacgc ttgggcgtca
      781 gcaccaggtg aagaaagaaa ctctagactt gaatcgcgtc gcggccgcgt gcgcgagcgg
      841 cgcgctggac cgacccgacc gcatcgagct cgtcttggag ctcggtccgg agcccctgtg
      901 ggtgaacgcc tccgactccc acgtttcccg tgccatcacc aacctgctgc gcaatgccgc
      961 cgaagccatc cctgaacgcg gtcagatcac cctgaagacc tggggtgaat acctgggaag
     1021 tgtgttctcc gggtatgagc tggtcgagcc gggagagtat gccgtgctct cggtgcgcga
     1081 ttccgggcag ggcatcgcgc cggaggtgtt gaagcggatc ttcgaaccct tcttcagtag
     1141 caaaaagctc cgtgacggca gtggctcggg gctcggtctc gcgatcgtac acggcgtggt
     1201 caaggagcac ggcggctaca tcaacgtgat gagccgacct ggcttgggca cggagttcac
     1261 gctgtatttt ccgaaggccc cgagtgcgcc gaaagctctc gccgagcccg cgattctacc
     1321 ggggaaaggg cgtgggaagc tgctggtgct ggacgatgat cccatgcagc tgcgcacggc
     1381 caagcggatc ctgtcgcacg cgggctatga cgtggtgacc gccgacagcg gtcagcgcgc
     1441 gctggccctg ctccgagaga gtgactcgag ccccgaagca gcggagccga gtggtagagg
     1501 ttcggcgagc ccgttcgatc tggtgctgct cgacatgaac ctcaacgagg ctgaagacgg
     1561 cctcgagatc ttcgaacgga tgcgttcgac ctaccccaat cagcgcggca tcctggtcag
     1621 cgggcatgca cctctcgaac gtggggagct cgctcagcat cgcggcctga cgtggctcag
     1681 caagccgtac acctccgatg cgctgctgct cgccgtgcaa acggtattgc tccaggttag
     1741 cccttcttat cgccccctct ggcatccttc ggacatcgaa caacgacacc tcagcttcgg
     1801 tcccccggag tcacaaagcg gagttcactg acgcttcgcc agccgaagct gctcaagatc
     1861 cgagaacgca gctatagccc gcgcacgtat tcagctgctt acaggtgtgc tcggtcagag
     1921 tgagcgtttc ggtatcgtag gagaagccgc gacaggtatt gagaccaccg cagtgagcgt
     1981 gcgtctccac gcgaccgccc cgcaccgtgc actcctcgga aaacttctcg agcgtcatgt
     2041 ccggctcgat tcgcgaggag gtcacggtgg cctcggtgtt ggctttctcg tcatccttcg
     2101 agccacaagc cgtgctgatg cacatcaaac cggcgagcgc cgccgcgagc atgccggggc
     2161 taggcgtgct cggcgctggt gcgggctcga gaatcgtgag gtgcgggcgc ggcgtcgaac
     2221 tcgacatcgg cctagtttga ctaggccccg ccccgcccgc aaggcctcct cggcttggtt
     2281 taccgacctc ggtttacggg cacctcagcc gcgcaccggg agattcggag gaacggtact
     2341 cgccaggctc gggcgttgca gctgctgcgc gatgtaacga tcgagctcgg ggtagccgtc
     2401 gagcagctcc gggcgacgca gcttcacata gccggtcgcc gcgatcaccg cgatgtccgc
     2461 caaggtgaag cgctccccat gcacgaactc acgccctcgg agcgcctgct cgatgtagct
     2521 caacgaggcc cgcaccttgc cctcgagctt ctgcgcgtag gccgcatccc gcagctcggg
     2581 ggcgcgccgc gagtccagaa cgaccggaat caacacatca caaatgccgt cgccgatcgc
     2641 ttcccaacgc cgcaccagcg cccgatcatg aacctccatg gggatgagac ccggtagcgg
     2701 gtgcatggcc tcgatcgttt cgatgatcac gcgcgagtcg aacaacacca agccatcatc
     2761 caagatgcac gtcgggacct tgcccaatgg attgtgttct gcggggctcc ggttagtggc
     2821 cgctacgtcg atcccttcga acgggagctg cttttccagg agcacacagc gaaccttgcg
     2881 agcgtacggg cttgtcggag aaaagtagag ctgcatcggg atggcgagac cctaccaccg
     2941 ctcacggatt atttgcgtga gaacgtgcgg gtttggtcga gactcgatgt cgtgcaccga
     3001 gtctcgtgag cctttggctc gggaccggcc tcgcgagctc gacggcttca gccgccgcag
     3061 gctgcggtaa tgagggcacc catcgcgctc ttttggctat cgatgtggct ccgtagcttg
     3121 ctgacctctg cttgccacac gctagccgaa gcgtggggat cgcccatcat ggcgggctct
     3181 acgaccttag ccatatcgtc gatgagcgaa gtcttgaaca catcgttcaa gtaagccttc
     3241 atggcagtgc aatactcggt cttgaacacg gtggtgaggc gcgtcacgag cggcggacga
     3301 atgccgcgcg aaccgaggaa actcgacacg gacttgaaag ggtcgcaatt cgacgggtag
     3361 cacttctggc gctgaaacgc cgtatccaga tcccacgggt acaccaggaa acgcccacca
     3421 cgaggagatg tggcgtccgg gtggaagtac agatagaaat tgcgtttgta gccgatgaaa
     3481 gactcgagct cgggagtcag tgattcgaac gcgatcacct tcagccactc ttgaacgtcc
     3541 agccattcac tgatgcaggt cttgaattgc gcgtcgtcgg gggtcgtgtc tttgtccgcg
     3601 cacttgaaca tcgggaacag gaatttctcc atgtcggcct gggtcccgcg ctccgccttg
     3661 taagccgcgg cgtaggtcgt attgctggta tcgccctcgt actcgaggtt ggccttgccg
     3721 tccgagaagc cacagtcact ctcggacggt gaggcctcca ccagcagccc ctcggacgta
     3781 ccccagtggt gccgcaagaa gcggccatgg tcggcctcct ccacgctctc gagcaagccg
     3841 taaaactggt cattcacgta cacccgcagg tggttgcagc gcggcgcttg caccccgaag
     3901 tcgcgcagcg ctttgtaggc caggcactgc accatgtgcg aatcatcatc ctcgatcgaa
     3961 aggatgttca cccgattcaa atccgcgaaa cgccgccgcg catcgccggt cgcagtgccg
     4021 agcaacttct ggaaatcgag cttgaagccc cgcaccggtt gctcgcggct accccgcagg
     4081 cgaataccga catcactgag ctcgaatttg ccgctcgagc cctcgtatac cacacgggaa
     4141 cgcacgtagt tgtcgaatgg gcggcagtgc ttccacttgg tcttgagcaa ggccagccag
     4201 tcgctcgcag cgtgacctgc cagatccgag ccctcgatat aaactttgag agttgcgacc
     4261 tcgttgttgt tgaagaaagc ttcgtaactc ggaccgcacg ccggcgggca cacctgagcg
     4321 gcgccgccgc tgcccgcgcc tgcaaagccg gcgctgcccc cgccaccgaa gctcgcgccc
     4381 gcaaacgcgc tgctgccagc cgcacctccg aacgccaaag taccgccatt cgcgccggct
     4441 tgtgccccga atccggcgcc tccggacagt gcacccgccg tcccccccgc accgcccgcg
     4501 gtgctcatgc tgccggcgag agagccgccc gcaggagagc caccaccaga gtggcctgcc
     4561 gccccgccgc tattgctcgg agacatcgtg tcattgggag acgtcgagtg cgaacaagca
     4621 gagagcgcaa ctacggctga gacagccagg gtcgagacga tccaggagca atgactcatt
     4681 gacggccttg tgggtcgaga cgtgtggacg ggcgtgcgtg cgtcgcgcgc acgcctcttc
     4741 gcttcggtgt gggaatccca ggtgcgagca ttcagcctgg gctggcgagg tccccagcgc
     4801 gcgaacgtgg gaagcgcgcg ctgggtccca cttcgaatga agggtcgaga tatgaagcta
     4861 ggtgtcccgt cagccaccgc ggcaggcaac aggagcaagg cttttagatg acgaaatggc
     4921 gaaatttccg ccagttccta gtatttactt gactccttaa agtgcacgag cagcccaggc
     4981 gccccgcgtt ttacccaacc cacagaattc ccctccaacg cgattcagtt gcaggcagtg
     5041 gctacgtctt cgttttcgga cgagcacgcg gtctcgaccg cggcgctgtc gcaaacgttg
     5101 ggctgcttca tttcacaagc ccacaccact tgtgaagcgg cctggcagct cgccaacttg
     5161 ttgtcgggca caccttggaa ttggcacttg tagtcgtagc aatcggtcag gctcaggtcg
     5221 gtctcgcaag ctacgcgctt ggcgcaatag tcgtgacaat ccttttccca tgcgggcaac
     5281 gcagtgctgc cgccggtcga agaggagccg ccgctcgagc tcgacgtccc accgctgccg
     5341 ccgctgccac ccttcgcggg actatcatcg tcatcgccac cgcaaccgac cagaccgaac
     5401 accgagaagg ccactgcaag cacggcacag gttttcttca tcatcaggtt tcctccacgc
     5461 ccaagcgcaa agtgacgcgc tggccacggg cgtatagcgc atttgtgact tcaggcacta
     5521 ctgagcaggc tccgactggt gccgcagtgt gcgagtgtgt acgcgcgctg cttggttttt
     5581 cgcgcgagcg gttactgccc ctcgagagag gcaaaggttc aaagtttcac ccgcgcgtct
     5641 ctggcccttg ctgaccggag gtggcctctt ggcgcaaggt ctcgttatcc caaccagcct
     5701 cgtattcgcg cttgatccgc gcggggtcct cggcacgcat caaacgctcg gcttgttcca
     5761 gcgcacggcg agcggccgcg aagtagctgt cttgatcgag atcgtaaagt gactcgagat
     5821 ctcttagcgc cccttcctcg tggcggcgcc acacgtcagc caagcgttgg gccgtgtgcg
     5881 cgcgataacc caggctgcga aatgccgcga ccccgagctc gaaggcgctg ccaaaggttt
     5941 cgcgaaacac ctcgtctact ccggctctgc gcaaagcata gtagtgctcc cgattgcgcg
     6001 cacggctcaa aatccgcagc ttcgggaagt gcttgcgcag cattttggcg agctccagcg
     6061 cctgctccat atcgtcgatc gcaattacga atagcttggc gtgctcgcaa ccagccgcat
     6121 acagcagctc gggtctggat gcgtccccgt agtacaccgc atgccccagg cggcgcagta
     6181 agctaaccat acccggatcc aaatcgagca cggtggtgcc aatgccgctc gcgcgcagca
     6241 tacggccgac gatttgaccg aagcgcccaa accccgcgat caccaccggc tggtgtccgt
     6301 cggtaatctc gtcgtcgtcg cggcgcgggc tactcgaggt ggcacgtggc aaaatgctgc
     6361 gttcgaggat caagaaagcg atgggcgtga gcgccatgct g
//
LOCUS       DASTKU010000561         9056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_331969, whole genome shotgun sequence.
ACCESSION   DASTKU010000561 DASTKU010000000
VERSION     DASTKU010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9056
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331969"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFQ61_19420"
     CDS             <1..198
                     /locus_tag="VFQ61_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956680.1"
                     /translation="IESVTLNGTNATRSGNDSGGGNAWVASGQSPPCTVKAKDAVGNQ
                     LEATFSGGNMLQDVGKQFTCQ"
     gene            348..1340
                     /locus_tag="VFQ61_19425"
     CDS             348..1340
                     /locus_tag="VFQ61_19425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956681.1"
                     /translation="MTSLVAMVEERFARLPGSAGWKLELSFPLPVGWPARSYVRYAYG
                     SRLRPGLADGIEVAAPWARLDASDPSIFEYLADRVSLLGIQGVHPMSRGELDLIAEIN
                     RAGTVDDLLARAPHDPSAAELVRRYYCNWSKQLIPAQLLRHHPEFATFLDCGNVQALR
                     EPAPKASPDVLLYSEFNKRWPNSPSRRWMPRLTPAFPMEWPASPRTNLCYYAHAICVD
                     RSLPVPCPREVLAPWGVILRNGRGGSLAFFASQTEARSLGMQAGRSPPPDWLETRRAL
                     GEDEEYALLQATSDPVRGGAVRRFYREWMLDEPLLAPAIIARHPEFFAFVTNDG"
     gene            1603..3534
                     /locus_tag="VFQ61_19430"
     CDS             1603..3534
                     /locus_tag="VFQ61_19430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956682.1"
                     /translation="MSLKRCATVLSFFSFLIALPRVSGATTIYVGPNGDANAAGATRD
                     APTTPAAAAAKVSPGDVVIYLDGVYKGFVNLSRPGTADAPITFRADDGAVPIIRGNGI
                     DENQGAINADPNGDVGWYVFEGLWFEEWGSGGIELDWANTKCHDLTIRYCVADRNGRN
                     GFSPYNSNNVTLEYNLASRNGYRPDSWSSNFNLFGVRGKVMVRGNVAFHGIDTSSNKS
                     DGNGFIIDLALANADVTFENNIAFANGGACIAVTDSGNTKLIGNTCYQNVWDAASNVQ
                     EFNFVNTCRGKIDDTNIDVAGKPWTFSSLVARNNAVIYRQGKQGYTTITSCGGGTTWT
                     NENNYFNGNAGIYQDPAAGDFRPKAGSAIVDKVAHGMTFATDIGFDPKCIKLETDESK
                     KIGNGVKRDWWTHAPDLDYIKKIGGIRNCWSPRTRPQGTNQEIGAYEVSTTGCTTAAS
                     CDDKNPCTRDTCSAEGTCTNAAIDGCGMGGAGGSPGAGGGSNGGASSGGTNAGGTATA
                     GSGGASSAGGSNGGASGSGGAAGAAGAALTGGQTGTGGGSLGGNSAGGTISAGQPSTG
                     GGGLSGAAAGGASGGVTARGGASGLAGASATPTSPSGDSGCSCSVPNSRHRESTAWGM
                     LALVGLGFVAVKRRDARKS"
     gene            complement(3738..3811)
                     /locus_tag="VFQ61_19435"
     tRNA            complement(3738..3811)
                     /locus_tag="VFQ61_19435"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3775..3777),aa:Met,seq:cat)
     gene            complement(3920..5152)
                     /locus_tag="VFQ61_19440"
     CDS             complement(3920..5152)
                     /locus_tag="VFQ61_19440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9956683.1"
                     /translation="MHAFLVAFGAALWSARSGKAPEPGPREVSIDVANEGVGPNPNPS
                     RASALASSAVEEVRPPGGSRVARPDVGKTGRGGTRDGAPATNLASSVDPLTLERDPPN
                     HLARSEVQRLRTGGDRRSLDDRRATPNPMELSFVASGRGRLALRRPDAPQVPAKGERI
                     GGVAAVDGAQHPLQFGDDSRPVAEREGAEARRALGAIHGEVEPRPRASASVLLARPWV
                     PKSRAAVPALKRDQPSDTVESEQLASSPTRALQQASTLGGEANRGVGGEPVAAFPALG
                     SGDQWGSASAPSGAGNGPPDFASDPSLQGFYRGVLSHLQRVLDRTFPDWAIAQGRGGL
                     VVFDLTLTDAGRVASVSVIRPSGIEEYDRNVVLAVRRVPGFGPVPPALGTRAVLRISW
                     DSRNPAVGRNGPGPGRAD"
     gene            complement(5204..6004)
                     /locus_tag="VFQ61_19445"
     CDS             complement(5204..6004)
                     /locus_tag="VFQ61_19445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET9956684.1"
                     /translation="MWFDTHCHLDSDDPARPTALLLERARRAGVTAFVNIGVGGVAAM
                     RGAVALAVAERNVWATVGVHPHEASSWAPEIELELLELLGEPRVVALGEVGLDYHYDS
                     SPRDVQRRVFAEMVGVACRVNKPLVIHTRSAPQETLDILRAESAERVGGIIHCFSEDI
                     AFARAALDLGFDISFSGIVTFKKAEAIQQVAAFVPSDRLLLETDSPYLSPVPLRGKPC
                     EPAFLVHTARRVAELRCVELSDLARVSTENAVRRFGVSLELEPAIAHN"
     gene            complement(6066..7391)
                     /locus_tag="VFQ61_19450"
     CDS             complement(6066..7391)
                     /locus_tag="VFQ61_19450"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9956685.1"
                     /translation="MKQPVGALSLLALGLNGIIGVGIFFTPNLIAAQLPGRAAMWAFP
                     ATALALLPIAVAFATLGGRVGLDGGPYVWARAAFGSRAAAWVGWVAFVSALLSTAAVF
                     AGLRDFLAADLGLRGAGEKAAFTAACVFAFAVIAALGLRPSTWTWNLLTVLKLVPLLL
                     LGASALSVGLGLWPRPTLLTESIGPPSLGATRPMATAFGSIEFERLARALLLCVFPLQ
                     GFEIVPVLSGSARSPRAIAVATVGSLLGAAGLYAWIVAACVDAVPALATSSSPLTDAA
                     YALGGTSLRRVVAWGTHVSALGIAFGMIVMTPRYLAALGNPSALGARLGDLDSRAVPQ
                     RALWLCASIVAVLVSVQGLASLFVLSSSAVLIQYVAALAALLLFGWQRRFGLTRRSLV
                     WPLAGTIAVGSLMHAVELRELVVLASALLLGGLLLYARHPARAPKPPTE"
     gene            complement(7388..>9056)
                     /locus_tag="VFQ61_19455"
     CDS             complement(7388..>9056)
                     /locus_tag="VFQ61_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protein kinase PrkA"
                     /protein_id="HET9956686.1"
                     /translation="LMEVRDHPLFLLPRLERRRLLERMYRENGMQDPPPAWVMHGELS
                     HKSRLVYEALLTTYDGSLEQVLRHVQVERYFISRRYRVGAVTLGPQLSVDAGERQITA
                     DRSLGALPTALQAVTLFEAFGELVDASGGLLEFSDLLKRPLDAFKYLQITAETGEVAL
                     RSQTLSVNCVMLASGNEVHLAAFREHPEFESFRGRFELVRAPYLLSYVDEQAIYDAQV
                     ASQVRGHVAPHATEVAAVFAVLTRMRRPNADRFQKPLRDLFSDLSAVEKADLYAKGVP
                     PTRFDDESKKLLRNAVEELYTESDAYPIYEGSVGASPREMRSVILNAAQNPRFSCLSP
                     LAVLDELDALCEKVGEYAFLQEEKLPGGYHDHALFRRTIRERLLDSFEDEFRIASGLV
                     DEAKYNELFDRYVTHVSFWVKNEKLRNPLTGQYEDPDERMMREVEALLGNPQRPEAFR
                     HGLIGTIAAWAIDHPDQPIDNTTVFAEPLRRLREAVFAERRTSVAKLCRDMLVVLRES
                     GAGLDDARRAAAIRTLETLKSRFGYEESSAVDAAAALVRERFHKILP"
BASE COUNT         1744 a         2991 c         2857 g         1464 t
ORIGIN      
        1 atcgagtcag tgaccctcaa tgggaccaac gccacccgtt ccggcaatga ttcgggcggc
       61 gggaacgcct gggtcgcgag cggtcaaagc ccgccctgca cggtgaaggc caaggacgcg
      121 gtcggcaacc agctagaagc cacgtttagc ggcgggaata tgctgcagga cgttggcaaa
      181 cagtttacct gccaatagaa gccacccgcc cccctcgcga caaactccgg tcagcgggcg
      241 atgagtagcc gatctccgta accagcaaac agcccggggc tgctggactg acccagcccg
      301 gggctgctgg actgaacgag tgcgctactc tgagctcgag gccgctgatg acgtcgttgg
      361 tcgcgatggt cgaggagaga ttcgcgcgct tgcccggttc tgcgggttgg aagctcgagc
      421 taagctttcc tttgcctgtg ggatggccgg cgcggagtta cgttcgctac gcctatggat
      481 cacgtctgcg tccaggactg gcggacggca ttgaagtcgc tgccccctgg gcacgactcg
      541 atgcgagcga cccatcgatc ttcgaatatc tggcggatcg ggtctcgctg ctcggaattc
      601 aaggggtaca tcccatgtcg cgcggggagc tagatctcat tgcggagata aatcgcgctg
      661 ggaccgtcga tgacttgctg gccagggccc cgcacgatcc cagcgctgcg gagctagtgc
      721 ggcgctacta ctgcaattgg tcgaagcagc tcattcccgc ccagctctta cgccaccacc
      781 ccgagttcgc aacatttctc gactgcggga acgttcaggc gctccgcgag cccgcgccga
      841 aagccagccc cgatgtgctg ctgtattcgg agttcaacaa gcgttggccc aactcgccca
      901 gtcggcgttg gatgccacgc ctcaccccgg catttcccat ggagtggcca gcctcgccgc
      961 gaaccaacct ctgctattac gcgcacgcaa tctgcgtcga tcgaagcctg ccagttccat
     1021 gtccccgcga agtcctcgcg ccttggggcg tgattctccg caatggtcga ggcggatcgc
     1081 tcgcgttttt cgcgagccag accgaagcgc gctcacttgg gatgcaagcc gggcgctcgc
     1141 ctccgcccga ttggctcgag acccgccgcg ccttgggtga agacgaggaa tatgcgctcc
     1201 tgcaagccac ttctgatccg gtgcgcggcg gcgcagtccg acgcttttac cgagagtgga
     1261 tgctcgatga gccgctcctg gcgccggcca tcatcgcgcg tcaccccgag ttctttgcct
     1321 tcgtcaccaa cgatggttga gccccggtgc gggtctggcg gatggcgtcg cagtctgcgc
     1381 acccggctgc ctcccgtagt gacgagactc acgtctcaaa atcacggtcc atgtctcata
     1441 aagttcagac cggaatcgtg cgcgggctcg ctggaggtgc gtttgaaacc tgcctcggca
     1501 cacgacaggt gagctcgcgc acagagcatt caagaatccg aactagttcg ttgcgggctc
     1561 aatgcgacga ggtcacaaat gggaactccg taggtccgcc ccatgagtct aaagcgctgt
     1621 gcaacggttc tttccttctt tagttttctg atcgcccttc cccgcgtgag cggagccacg
     1681 accatctacg taggcccgaa cggcgacgcg aacgcagccg gggccacgcg cgatgcgccc
     1741 accacgccag cggccgcggc cgccaaggtt tcccccggtg acgtggtgat ctacctggac
     1801 ggcgtttaca aagggttcgt caacctctcg cgcccgggca ccgcggacgc accgatcacc
     1861 ttccgtgcag acgacggcgc ggttccgatc atccgcggca acggcatcga cgagaatcag
     1921 ggcgccatca acgccgatcc gaacggcgac gtcggttggt acgtcttcga agggctgtgg
     1981 ttcgaagagt ggggcagcgg tgggatcgag ctcgattggg ccaacaccaa gtgccacgat
     2041 ctgacgatcc ggtactgcgt tgccgaccgc aacggccgaa acggcttctc gccttacaac
     2101 tcgaacaacg tgacgctcga atacaacctg gcgagccgca acggctatcg gccggattcg
     2161 tggtcgagca acttcaactt gtttggcgtg cgaggcaagg tgatggtgcg tggcaacgtc
     2221 gcattccacg gcatcgacac ctcgtccaac aagagtgacg gcaacggctt catcatcgac
     2281 cttgccctcg ccaatgccga cgtcacattc gagaacaaca tcgctttcgc caatggtggt
     2341 gcgtgcatcg cggtcaccga ctcgggcaac acgaagctga tcggaaacac ctgctaccaa
     2401 aacgtgtggg acgctgcgag caacgtgcag gaattcaact tcgtgaacac ttgtcgcggc
     2461 aagatcgacg acacgaacat cgatgtggcg ggtaagccgt ggacgttctc gagcctggtc
     2521 gcacgcaaca acgccgtgat ctatcggcaa ggcaagcagg gctacacgac catcacttcg
     2581 tgcggcggag gcacgacgtg gaccaacgag aacaattact tcaacggcaa cgccgggatt
     2641 taccaggatc ccgccgccgg cgacttcaga ccgaaagcag gctccgccat cgtcgacaag
     2701 gtggcccacg gcatgacctt cgctaccgat atcggcttcg acccgaagtg catcaagctc
     2761 gagaccgacg agagcaagaa gatcggcaat ggcgtgaagc gcgactggtg gacccacgcg
     2821 cccgatttgg actacatcaa gaagattggc ggcattagaa actgctggtc gccacgcacc
     2881 cgcccgcaag gaaccaatca agagattggc gcttacgagg tttcgaccac gggctgcacg
     2941 acggccgcgt cttgcgacga caagaatccc tgtacacgag acacgtgcag cgccgaaggc
     3001 acgtgcacca acgcggctat cgacggctgt ggaatgggag gcgctggcgg cagccccggc
     3061 gctggaggcg ggtcgaatgg cggcgcctct tcgggtggga cgaacgccgg tggtaccgcg
     3121 accgcgggta gcggcggcgc ctcgagcgct ggcggctcga atggcggagc ctcgggtagc
     3181 ggcggcgcgg cgggagcagc gggtgcggct ctaacgggag gccagacggg cacgggtggc
     3241 ggcagcctgg gcgggaacag tgccgggggc acaatctccg cgggacagcc ctcgacgggc
     3301 ggcggcgggc tgagcggagc cgccgcgggt ggtgcaagcg gcggggttac tgctcgcggc
     3361 ggtgcgagcg gcttagctgg cgcctcggca acgcccacgt cgccgagcgg tgactcgggt
     3421 tgctcctgca gcgtgcccaa ctctcggcac cgcgagtcga ccgcttgggg aatgctggcg
     3481 ctggtcggtt tgggattcgt cgcggtgaag cgccgtgacg cccgcaagag ctgaagcccc
     3541 acaggctcgc ttcggcgcga tcccgccact ttggggggat cgcgcgccgg acttagaaca
     3601 ggtcaaccgg tacgtccacc gtgccaactt caagacggaa gcaccacgtc gcccgacgtg
     3661 agccggtgga cggcgccatc aaaatggcgg cgccgcccaa tggcgaagag ttcgagcccc
     3721 tgagaacccg gaatcggtgc cgaaggtcgg acttgaaccg acgaccccac gcttatgaag
     3781 cgcgtgctct aaccagctga gctacttcgg cgacgggcgg ggacatatta tcttcgggaa
     3841 atgcctgtca agttggctct tcggccgccc acaaatattc ggacttggcg ggttcggtgg
     3901 gcaggccgtt cggagcactt cagtcggccc ggcccggacc ggggccgttc cgaccgacgg
     3961 cgggattgcg agaatcccag ctgattcgca gcacagcgcg agttccgagc gccggtggta
     4021 cggggccgaa cccgggaacc cgccgtacgg cgagcacgac gttccggtcg tattcctcga
     4081 tcccactcgg tcgaatcacg gagacgctcg cgacccgtcc cgcgtcggtc agcgtgaggt
     4141 cgaacacgac gaggccaccc cgcccctgtg cgatggccca gtcaggaaac gtgcggtcca
     4201 gcacgcgctg caagtggctc agaacgccgc gatagaagcc ctggagactc gggtcactcg
     4261 cgaaatcggg cggaccattg ccggcgccgc tcggcgccga ggcagagccc cattggtcac
     4321 cagagcccag cgccggaaac gcggctacag gctcaccgcc aacacctcgg ttggcttccc
     4381 cgccgagcgt actggcctgt tgcagcgccc gagtcggact cgaagccagc tgctcgcttt
     4441 ctacggtgtc gctcggctgg tcccgcttga gcgcgggaac tgcagcgcga gacttcggaa
     4501 cccaaggtcg cgccaacagc accgaagcag acgcgcgagg gcgcggctcc acttcgccat
     4561 gaatcgcgcc gagcgcgcga cgagcttccg ccccttcgcg ttcggcaacc ggtcgcgagt
     4621 catctccgaa ctgcagcggg tgttgcgctc cgtcgaccgc ggcgacgcca ccgatccgtt
     4681 cgcccttcgc cggtacttgc ggggcgtccg gtcgccgtag cgccaggcga cctcttccac
     4741 tcgccacgaa cgagagttcc atcgggttcg gagtcgcccg gcgatcgtcg agtgagcgtc
     4801 ggtctccccc ggtgcgcagg cgctgaactt cgctgcgtgc cagatggttc ggggggtcac
     4861 gttccagcgt gagaggatcg acactactcg cgaggttcgt cgccggtgca ccgtctcgcg
     4921 tacctccgcg gccggtcttg cccacgtccg ggcgcgccac gcggctcccc cccggcggcc
     4981 tcacttcctc tactgcggaa ctggccagtg ccgatgcacg ggaaggattg ggattcggtc
     5041 caacaccctc gttggcgaca tcgattgaaa cttcacgcgg gcccggctcg ggtgctttgc
     5101 ccgagcgcgc agaccataac gctgctccaa acgctacaag gaaggcgtgc actgccagcg
     5161 aagcaatcca cgctggcggg tcgacgcgcg aaagttgcac gcctcaattg tgagcaattg
     5221 cgggctctag ctcaaggctg accccgaaac gccgaacagc gttctctgta ctcacccgtg
     5281 ccaggtcgga tagttctaca caacgcagct ccgccacacg gcgcgccgtg tgaacgagaa
     5341 acgcgggttc gcagggcttt ccgcgtaatg ggacgggtga caagtagggg ctgtcggtct
     5401 cgagcagcaa gcgatccgag gggacgaaag ccgccacctg ctggatcgcc tcagccttct
     5461 tgaacgtaac gattccggag aaggagatgt cgaagccgag atcgagcgcg gcccgcgcga
     5521 aggcgatgtc ttcgctgaaa cagtggatga tcccgccgac gcgctcggct gactccgcgc
     5581 gtaagatgtc gagtgtttct tggggcgcgc tgcgcgtatg gattacgagc ggtttgttca
     5641 ctcgacaagc gacgccgacc atctccgcga acacgcgtcg ttgcacgtcg cgagggctcg
     5701 agtcgtagtg ataatcgaga ccgacctctc ctagagccac gacccgaggc tcgccgagaa
     5761 gttccagcag ctccagctcg atctccgggg cccagctgga cgcctcatga ggatgaacac
     5821 caacggttgc ccagacattg cgctcggcga ccgcgagcgc gaccgcacca cgcatggccg
     5881 ccactccgcc gaccccgatg ttgacgaaag cggtgacgcc agcccgtcgg gcgcgctcca
     5941 agagcagcgc ggtgggccgc gccgggtcgt ccgaatcgag atggcagtgc gtgtcgaacc
     6001 acatgcgaag accgtatagc gcgagccgcg caggggacca gcggcgccga ccttcgcgca
     6061 gtccttcact cagtgggagg cttcggagca cgcgcgggat ggcgcgcgta gagcagcagg
     6121 ccccccagca gtaaggccga cgcgagcacc acgagctcac gcaactccac ggcgtgcatc
     6181 aacgagccca ctgcgattgt tcccgccagc ggccatacca ggctgcgacg agtgagccca
     6241 aaacggcgct gccagccgaa aaggagcagg gcagcaagag cggcgacgta ttggatgagc
     6301 accgccgagc tcgacaacac gaacagactc gcgagccctt gtaccgatac gagcaccgcc
     6361 acaatcgagg cgcacagcca gagagctcgc tgtggcaccg cgcgtgaatc caagtcaccg
     6421 agccgggcgc ccaacgcgct cgggttacct agtgcggcga gataccgcgg agtcatcacg
     6481 atcatcccga acgcgatccc gagcgccgag acatgcgttc cccacgcgac gaccctgcgc
     6541 aacgatgtgc cacccagcgc gtaggcagcg tcggtcaacg gggatgaaga cgtggcgagc
     6601 gcgggcacgg cgtccacaca cgcggcgaca atccaggcat aaagccccgc cgctcccaag
     6661 agcgatccta ccgtcgcgac cgcgatcgcg cgggggcttc gggcgctacc cgacaacacc
     6721 ggcacgatct cgaagccctg caatgggaac acacacagca ggagggcgcg tgccagcctc
     6781 tcgaactcga tcgatccgaa cgccgttgcc atcgggcgag ttgcgccgag cgaaggaggc
     6841 ccgatcgatt cggtcaacag cgttggacga ggccaaagtc caagccccac cgagagcgcg
     6901 gaggctccca gcaatagcaa cggcaccagc ttcagaacgg tgagcaggtt ccaggtccac
     6961 gtgctcgggc gcaacccgag cgccgcgatg acggcaaacg caaacacgca tgcggccgtg
     7021 aacgccgcct tctcccctgc ccctcgcaat ccgagatccg ctgccaagaa gtccctcaag
     7081 cctgcgaaca cggcggccgt gctgagcaac gccgaaacga acgcgaccca gcccacccac
     7141 gccgccgctc tgctgccgaa tgctgctcta gcccaaacgt acgggccgcc atccaagccc
     7201 acccgaccgc caagcgtagc gaaggcgact gcgatcggca gcaacgccag tgccgtggcc
     7261 ggaaaagccc acatcgctgc gcgccccggc agctgcgccg cgatcaggtt aggcgtgaag
     7321 aatatcccca ctccgatgat gccgttcaag ccgagcgcga gcagggatag cgcgccgacc
     7381 ggctgcttca cggcaagatc ttgtggaagc gttcccgcac gagcgctgcc gccgcatcca
     7441 ccgcggagct ctcctcgtaa ccgaagcggc tcttcagagt ctctagggtt cgtatcgccg
     7501 ccgctcggcg cgcatcgtcg agaccagcgc cactttcacg cagcaccacc agcatgtcgc
     7561 gacacagctt ggcgacgctc gtgcgacgtt cggcgaatac ggcctcccgc aagcgacgga
     7621 gcggctccgc gaacaccgtg gtgttatcga tgggctgatc ggggtgatcg atcgcccagg
     7681 ccgcaatggt tccgattagc ccgtgtcgga atgcttcggg gcgttgggga ttgccgagca
     7741 aggcctccac ctcgcgcatc atgcgctcat ccggatcttc gtactggccg gtgagcggat
     7801 tgcgcagctt ctcgtttttt acccaaaagc tgacgtgcgt gacgtagcgg tcgaacaact
     7861 cgttgtactt ggcttcgtcc acgagaccgc tcgcaattct gaattcgtcc tcgaaagagt
     7921 ccagcaggcg ttcccgaatc gtgcgccgga acagggcatg atcgtggtaa ccgccaggca
     7981 gcttctcttc ctgcaagaac gcgtactcgc cgaccttctc acagagcgcg tcgagttcat
     8041 ccagcacggc gagcggagac aaacaggaaa aacgtggatt ctgagccgca ttcagaatca
     8101 cactccgcat ctcgcgcgga ctcgccccaa cgctaccctc gtagatggga taggcgtcgc
     8161 tctccgtgta gagctcctcc accgcgttgc gcagcaactt cttggattca tcgtcgaagc
     8221 gcgtcggtgg cacgcctttg gcgtacaggt cggccttctc gacggcgctc agatcgctga
     8281 acaagtcacg cagaggcttt tggaaccggt ccgcattggg gcgccgcata cgggtcagca
     8341 ccgcgaaaac ggccgcaact tcggtggcgt gcggcgcgac gtggccgcgc acctgggacg
     8401 ccacttgcgc gtcgtagatt gcttgctcgt ccacgtaact gagcaagtag ggtgcgcgca
     8461 cgagctcgaa tcggccgcga aagctctcga actccgggtg ctcgcgaaac gccgccaggt
     8521 gcacctcgtt accgcttgcc agcatcacgc aattgacgga cagcgtctgc gagcgcaacg
     8581 ccacctcacc tgtctccgcg gtgatctgca gatacttgaa ggcgtcgagc gggcgcttca
     8641 acaagtctga aaactcgagc aagcccccgc tggcgtccac gagttcgccg aaagcttcga
     8701 acagcgtgac cgcctgaagc gccgtcggca gcgcgcccaa gctccgatcc gccgtgatct
     8761 gacgctcgcc ggcgtctacg gagagttgtg gacccagggt cacggcgccc acgcggtagc
     8821 gacgtgagat gaaatagcgc tcgacctgca cgtgccggag cacttgctcg agcgagccgt
     8881 catacgtcgt cagcagcgcc tcgtacacca gccgactctt gtgggagagc tcgccgtgca
     8941 tcacccaggc cggcggcgga tcttgcatgc cgttctcgcg gtacatccgc tcgagcaacc
     9001 gacgtcgctc gagcctcggc agcaagaaca gtggatggtc gcggacctcc atcagc
//
LOCUS       DASTKU010000562         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1001014, whole genome shotgun sequence.
ACCESSION   DASTKU010000562 DASTKU010000000
VERSION     DASTKU010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001014"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2315
                     /locus_tag="VFQ61_19460"
     CDS             <1..2315
                     /locus_tag="VFQ61_19460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HET9956687.1"
                     /translation="AKVGGPLPAASNLLGRLSWVEYPIGARHYSYDERGSVQHRAEDL
                     WDGISPFDVQVRTTQVRRSYYDATATEVGRDLPGGFTTRSTLNARGLVASVQAGLSDG
                     QRTVTSGVRYDERGLVTRLDQANGLRQCRKYDERGALTHVLLATAVDANCEPQQLTQA
                     GFGLFHLRYRWGFDALLKGTEDLSRTRAGADRLDASYGYDRLQELVSATTADGSFGYG
                     YDAIQNLTRRTSQQGSGAPIEETLVYGEAGAGPNAVTHVGGTELGYDAAGQLKAYNGY
                     TLKFDAAGRLKRAQKSGGKTIEYYYDAFGDRKLLISIDGTGSRRIYRYPFEDFEERNG
                     SEAWRVDTPGGEVELRRSNGLELDAVLIDELTAYVNAPSGQPQPLPREWLDLDGDGDG
                     FDAQDLAVAMQGFWNGTRPGGRRVEWRYEQADQIESALLVTDSTGQEVSVRRYGPYGA
                     MVDRRGVSPSRGFAGAELEPDEDLGLTRIGARYYAPGIGRWVTPDRFIGESPERMAAD
                     PLQTNLYAYARNNPIVFTDPTGFDVTVKAKAGKLQDVKELKPGQKEPTGKDADGEYDG
                     GELSVGAHTKTTYDKETDTTKVVITYDVSVAAGSVLYKTGADPQNVDAEDAGRTLQEH
                     EYGHAGIALEIGASKEVIAELLDSVQATSEITLPGKPSSAELSAAMSLHSTAVTNYLI
                     ETLDYVDHAVNHKELADRYTAPNAPVYVDPESPPDNPRRASPSEAALGHATEAASRGR
                     AKGEAFAKSKAEKAKKAANKPAKKDESKKK"
     gene            2312..3694
                     /locus_tag="VFQ61_19465"
     CDS             2312..3694
                     /locus_tag="VFQ61_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956688.1"
                     /translation="MSVDAGRWWHFGAAVCAVLGLVRCVPVTPQRKLGQPNAIAPKPA
                     TPVNEQRATAPAAAPETYHWCDTAGLADISKEAESLEADIPRVSSDDEARALITRIHA
                     LGEQPCFRLAPKVRFSDAQSNAHSLRVFFARGGREWLHQAEAGRSREIIVPGPIPESP
                     ASSPSSPWVTFGLTCAEDDLACGGETRAWAQRAERVINAIGRRAPYSHLKDCRDQVRN
                     YDAWLECRLARAGLEWAFPVGPSLKAPQDGLLFSAASARDCTEWRLTDVASGSAVWAQ
                     SCGTKPPAVSLGSIDSRLLREALFVSALTDRVVWGPAFQRLVVPEEIALSSDLGVFPD
                     GFEVAAISSSLQVRHAWSYHRQDRTLFDGSLNWPEDLNRPERTYAVWLHDAADATWHS
                     GCRKRRDYQTLVDQLAVRLPRGTSVISLLRRELEANQCARLPRPMRLGYRSGWQSSRL
                     SLEEPFEPGE"
BASE COUNT          724 a         1151 c         1222 g          632 t
ORIGIN      
        1 atgcgaaggt gggtgggccc ttgcccgcgg ccagcaatct gctcggtcgc ctgagctggg
       61 tcgagtaccc gattggcgcc cggcactact cctacgatga gcgcggcagc gtccaacatc
      121 gcgccgaaga cctgtgggat ggcatctcgc ccttcgacgt gcaggttagg actacgcaag
      181 tccgccgctc ctattacgac gccacggcca cggaggtcgg ccgcgacctt cccggtggct
      241 tcacgacccg ctccacgctc aacgcgcgcg gtctcgtcgc cagcgtgcag gctggccttt
      301 ctgacgggca gcgcacggta acgagcgggg tcagatacga cgaacgcggc ttggtcacac
      361 gactggacca ggcgaacggt ctgcgtcaat gccgcaagta cgatgagcgc ggcgccttga
      421 cccacgtcct gctcgccacc gcggtggacg cgaattgtga gccgcagcaa ctcacgcaag
      481 ctggcttcgg attgttccac cttcgatatc gctggggctt cgatgccttg ctcaagggca
      541 cggaggacct cagccggacg cgagctggcg ccgatcggct cgacgccagc tacggctatg
      601 accgccttca ggagctcgtt tcggcaacca ccgcggatgg ttctttcggc tatggttacg
      661 acgccattca gaacctcacc cgtcgaacca gccagcaagg cagcggtgct ccgatcgagg
      721 agaccttagt atatggagag gcgggcgccg gacccaacgc cgtcactcac gtgggcggga
      781 ccgagctcgg ctacgatgcc gcgggccaac tcaaagccta caacggctat acgctgaagt
      841 tcgacgccgc gggtcgcttg aaacgcgctc aaaagagcgg cggcaaaaca atagagtatt
      901 attacgacgc tttcggtgac cgcaaactcc tgatctcgat cgatggcacg ggcagccgtc
      961 gtatttatcg ataccccttc gaagacttcg aagagcgcaa cgggagtgaa gcgtggcgcg
     1021 tcgatacgcc gggtggtgaa gtcgagctgc gtcgctcgaa cggactcgag ctcgacgcgg
     1081 tcttgatcga tgagcttacg gcctacgtca atgcgccgag cggccaaccc cagccgctgc
     1141 cacgggagtg gctggacctc gatggcgacg gggacggctt cgatgcccaa gatctcgccg
     1201 ttgccatgca gggcttttgg aacggaacgc gcccgggagg tcgtcgcgtg gagtggcgct
     1261 acgagcaggc ggatcagatc gagagcgcgc tactcgtgac agacagcacg gggcaagagg
     1321 tttcggtgcg tcgatacggt ccttacgggg cgatggtcga tcggcgcgga gtctcgccca
     1381 gccgtggctt tgcgggtgcg gagctcgagc cggacgaaga tctggggctc acgcgcatcg
     1441 gcgcgcgcta ttacgcgcca ggtattgggc gttgggtgac gccagaccgc ttcatcggcg
     1501 aatcccccga gcgcatggct gccgacccgc tccagacgaa tctctacgcc tacgctcgga
     1561 acaatccgat cgttttcacg gatccgaccg ggttcgacgt cacggtgaag gccaaagcgg
     1621 gcaagctgca ggatgtcaag gagctgaagc ccggccagaa ggagccgacc ggcaaagacg
     1681 cggacggcga gtatgacggt ggcgagctta gcgtcggagc gcacacgaag accacgtacg
     1741 acaaggagac cgatacgacc aaggtcgtca tcacctacga tgtcagcgtg gcggcgggct
     1801 ccgttctcta caagaccggc gcggatccgc agaacgtaga cgcggaggac gccggtcgca
     1861 cgctgcagga acacgaatac ggccacgccg ggatcgcgct cgaaatcggc gccagcaaag
     1921 aggtaattgc cgagctcttg gattccgtgc aggccaccag tgagattacg ctacccggga
     1981 aaccgagctc ggccgagctc agtgctgcga tgtcgcttca cagcaccgcg gtgacgaact
     2041 atctcatcga aacactggat tacgtggacc acgccgtgaa tcacaaggaa cttgcagacc
     2101 gctatacggc gcccaacgcg ccggtgtacg tggatcccga gagcccgccc gacaatccac
     2161 ggcgcgcgag tccctccgaa gcggcgcttg ggcacgctac cgaggccgcg agccgcgggc
     2221 gcgccaaggg tgaggcgttc gcaaaaagca aggctgaaaa ggccaagaag gcggctaata
     2281 agccagccaa gaaggacgag agtaaaaaga aatgagcgtc gatgcgggtc ggtggtggca
     2341 tttcggagct gcggtctgcg cggtgctcgg gctcgtgcgt tgcgtgcccg ttactccgca
     2401 gcgcaagctg ggtcagccaa acgcgatcgc accgaagccc gcgactcccg tcaacgaaca
     2461 gcgtgcgacc gccccagccg ctgcgcccga gacgtatcac tggtgtgaca cagcggggct
     2521 tgccgacatc tccaaggaag ccgaatccct cgaggcagat atcccgcgag tctcgagtga
     2581 cgacgaagca cgtgcgctca tcactcggat tcacgcgctc ggagagcagc cctgttttcg
     2641 gctcgcgccc aaggtgcggt tctccgacgc tcagagcaat gcccacagct tacgcgtgtt
     2701 ctttgctcga ggcggacgcg aatggctgca tcaggcggag gccggaagga gtcgtgagat
     2761 tatcgtcccc gggccgattc cggagtctcc tgcctcgagt ccgagctcgc cgtgggtcac
     2821 gttcggattg acctgcgcgg aggacgatct cgcttgcggt ggggagacga gagcgtgggc
     2881 tcagcgtgcc gaacgtgtga tcaacgctat tgggcgccgg gcaccgtatt cgcatctcaa
     2941 ggattgtcgc gatcaggtac gaaattacga cgcttggctc gagtgtcgcc tcgcgagagc
     3001 cgggctcgag tgggcgtttc cggtgggtcc ctcgctaaag gctccgcaag atgggctgct
     3061 gttctcggcg gcatcagccc gagattgcac agagtggcgg ctgaccgatg tcgccagcgg
     3121 gtctgcggtc tgggcccaaa gctgtggcac gaagccgccg gccgttagcc taggctcaat
     3181 cgactcaagg ctcttgcgag aggcgctgtt cgtttccgcg ctgacagatc gggtcgtttg
     3241 gggaccggcg tttcagcgct tggtcgtgcc cgaggagatc gcgttgagct cggacctcgg
     3301 tgtgtttccc gatggcttcg aggtcgcggc tatcagctcg agtctccaag tccgtcacgc
     3361 gtggtcgtat catcggcaag atcgaacgct gttcgacggc tccctgaatt ggcccgagga
     3421 cctcaatcga cccgagcgca catacgccgt ctggttgcac gacgccgcag acgcaacgtg
     3481 gcactccggc tgccgcaagc ggcgggatta tcaaacgctg gtcgaccaac tcgccgtgcg
     3541 ccttccgcgg ggcaccagcg tgatctcact gcttcggcgt gagctcgaag caaaccagtg
     3601 cgcccgactt ccgcggccca tgaggttagg ctatcgatcg ggttggcaaa gcagccgcct
     3661 gagcctcgaa gaaccattcg agcccggcga atgaacggtc gtgctaagac ccacccgagg
     3721 tgacggtga
//
LOCUS       DASTKU010000563        21646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1896013, whole genome shotgun sequence.
ACCESSION   DASTKU010000563 DASTKU010000000
VERSION     DASTKU010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 21646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21646
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1896013"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..867
                     /locus_tag="VFQ61_19470"
     CDS             151..867
                     /locus_tag="VFQ61_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956689.1"
                     /translation="MAKAHTEWTVLPHESLEKLADNLWWVRGSLKGMSLKRTMVIARL
                     ADGRLVIHNGIALDEPSMKQLEAWGNPSFLVVPSGVHRLDAPAFKKRYPGLRVIAPRG
                     ARAQIEQVLPVDLNYDEFPADDAVRFENLQGVGNSEGVMIVNSSDGASVVLNDVVFNM
                     DRKRDLLGFLFTTVMGSAPGPRVSRLGKLLLVKDKAALRADLERLAALPNLQRLIVAH
                     EKVASGPEASDALRRAATYL"
     gene            complement(905..2284)
                     /locus_tag="VFQ61_19475"
     CDS             complement(905..2284)
                     /locus_tag="VFQ61_19475"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9956690.1"
                     /translation="MPVMQQSNVERARVIPRVGHVRAGFAGLELVNLLQRDPVAFFSR
                     LAEQGDLVTCALAGQPFAQVTHPEHIQQVLVEKHRAFRKSQRVRRVLSQWSGDGLALI
                     DGERWVRVRRLVGPAFQSQLGPIHAAIILRRAREMVSRAKVSPSFDASREVSRLTFAV
                     AAEAMFGTEIDTHLDEFIGHVGNLNECAIRELNSLVWLPKWAPTPLKRRVRESAAYLE
                     RVVFGFIASARRQRPEHPTLLSVLLDSRDEDGSRLTDRELYDASVNVLLGAHETTAVS
                     LIWSLYCLSEHPEVQAAARAELLCVIGSGEVGPEHLSRLSLIEHVVKEAIRLYPPIYA
                     IPREVAEPVTIGGVALGAGTYVTIVPYITQRDARWFDGPEVFRPARFVRESEIPKGAY
                     LPFGLGPRACIGRSFALLEAVGTLAVLLREFSLHPSDPGSPVELQAQISLHPKGGLSL
                     VLQAPERRS"
     gene            2386..3948
                     /locus_tag="VFQ61_19480"
     CDS             2386..3948
                     /locus_tag="VFQ61_19480"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET9956691.1"
                     /translation="MPRSLRNAQRKAEAARLRELRGVLGLTQRELATEFNVAHGAIAA
                     WENGNRTLPGPVLKLMSLYEEELGLAPRAATLERLRTSRWARQAALSRTAGGILARVG
                     ATALERMLADDVHANAITARAHTAVARSLVDALGNLKGVALKFGQALSYLDFLLPEES
                     RRELQTLLVTSRAMSASVISQVVMEELGEPPRRLFSEWCAQPFASGSIGQVHRARLRS
                     GQEVAVKIQYPAIVQAIQADLAAASLMDRMTSLLFRAQQPGVVLDELRERFLEECDYR
                     IEAENQEQFRTLWRGHPKVHIPRVHRELSTSRVLVTDFAHGENFESFLQHASPAERDC
                     AGMLLWRFAFESIFRHGLFNADPHPGNYLFAQGRVVFLDFGCVKRFDPRMLGLWRGMI
                     RGVLEGDAETAHRLWMEAGMIPDPSRFDAEYHRHMLLTLYEPWLGAEPYRFEPEFVER
                     TWRALVLDNPNRFRTNLPKDWVFVNRLQWGLYAVLARLGAKGAFRNDLLDLLYSPHER
                     RPNPLTYTARLG"
     gene            4074..5180
                     /locus_tag="VFQ61_19485"
     CDS             4074..5180
                     /locus_tag="VFQ61_19485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956692.1"
                     /translation="MSKPRSVFWEQLILDGQSPVRQFLNAAAEHDGIGSNAFAFLPNL
                     RFAPTPEGGDVERIELAPLPALTAERQHELGRIVGRSLALWSWLGVSDLHWENLVLGV
                     DAGGHISFTPLDIEMMLADLALPTETKLLPDADPEYAETSRHACGMRRVLPYLGKPIE
                     ASALLEILGAYHGTLLFLERHGRDIGKLIAALPGLREAPMRVLLRGTDEYVNAHTREL
                     WPPLLEAEVEQLRRGDIPYFFRLYGRPGIHYYAEPTLQTFRTLPLEGDMPQLEPLLSP
                     SRGLSSGSRKRLREEGLFTVLGAFDHPSFGGKHGNGNLELVLSKRKVILTLPSGEEIH
                     ARRDLRAFVSSVYLPCRCGEVRTPFVPRLTRCEA"
     gene            5246..5848
                     /locus_tag="VFQ61_19490"
     CDS             5246..5848
                     /locus_tag="VFQ61_19490"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HET9956693.1"
                     /translation="MTVPACEVCSRPKVACVCDRVVQYETQRRVLILQHPQEQDALLG
                     SAQLLVACLPKVKLVVGLSWRNLAHALGEESVDPKRWAVLFPDRDSPGNCVTGRGGPI
                     DPAQLEGIIALDGTWSKAKTLWWRNPWLNKLHRLTLTPSKPSIYGRMRVEPRREFVST
                     LESVSAALTLCGEPPEIETGLSRIFRTLVQRVRDANIIPR"
     gene            complement(5915..7252)
                     /locus_tag="VFQ61_19495"
     CDS             complement(5915..7252)
                     /locus_tag="VFQ61_19495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9956694.1"
                     /translation="MFRFGIYAAPVAASGRGRARVIALGVAKAIDRNITERYERLGVL
                     GRGGQGTVYRARDRWMQREVAIKVLDSNAARDPHVSERLVREQRALSALKGTAAVEVL
                     DICRSNGGELCLVMELLAGTDLENHLYSVEDRHERIQPSWVADRLEPIVETLEVAHGA
                     GILHRDLKPANIFLLDDGSVRLLDFGMARLRRAAPLTAAGTCMGSPSFMAPESWQGQS
                     ERIDQRVDVYSLGVILFRVLAGELPFTGTSLQQKFMGATQSARPSLFAHRPDLPRDAD
                     EWVATALAIDPDQRFRNVRALWNAFIDVFEVKAARRRSQRAAFWAAAKRALHIAPAEA
                     SSAPAPPQRGNVNAQAEAPEKTDQTSTAPESSPASAPSQAITSAELEAVSISSLADSP
                     TEKPSERTLELSSRHLAPLLPPRRMPPPPPGRKKKEESTLEVDEIELESPETR"
     gene            7637..8230
                     /locus_tag="VFQ61_19500"
     CDS             7637..8230
                     /locus_tag="VFQ61_19500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956695.1"
                     /translation="MQKPTIAALLMLGCSAAFLGCSAEAEKKLAEYKKESDERIAKIE
                     SDAKSKLAAAEQKLSDTQRQYEELKTQLTEAKSKTEDAEKAKASAEEQAKLAEAAISK
                     ARQAFKEKAKLQLADLNKELTDAGVKASKAPAKAKTAFATAVQDVKKQQAAVAKDIAA
                     FDTATLETFKSINARIQTDLAVLKNKIRAARAKLPAS"
     gene            complement(8667..9719)
                     /locus_tag="VFQ61_19505"
     CDS             complement(8667..9719)
                     /locus_tag="VFQ61_19505"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9956696.1"
                     /translation="MFIPTVAVRLYRPVALALESRGVAAGPFFAEFGMPDPAVTGWDV
                     RLPLPQIAGVWGRLLEVTQDPEFALHAAQHVDLTVCDVITYLEGNARTVGEALRKKFE
                     YLPLITNAIEWTLDVSEGEAAITLHERPARPPLAPVAEFLLGTRQVFFQRFGPEDWGL
                     RAAYFRHEAPARIGTHTELFGVPPRFEAAQDQLVFDARLLEAPMRQRDDALSDILTRY
                     ADLVMKSLTPAETQTQRVEHVLRAGIDPGIAQVAQQLGVAARTLQRSLQLEGTTYAEI
                     AARMRRAAAERLLSRRELAISELAGALGFNDAPAFHHAFVRWTGTTPGEFRRQALGAN
                     YAEVGQGRLRAHPNRS"
     gene            complement(9806..11488)
                     /locus_tag="VFQ61_19510"
     CDS             complement(9806..11488)
                     /locus_tag="VFQ61_19510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956697.1"
                     /translation="MGGTPLVEVVFLSFVSSAACTPSDHWASRRQPDARAPAAVHARS
                     STADASPNEPRAGTTEPRAQPEESTPLPFHVIGEVPNNLHLYGAGERGFVTDEAKILL
                     DLVGNDVRHEARVVPELQHDLMYSLNGIVGRWPDAVWLSTSHPWGRSGYSQLWRWRGS
                     RWVRERKSNEAQFVFDIQPWSSGRMLAVLENSYLFDAAFWVLPGVAAARLVPHEGGAP
                     EFRRPASPEGFCQKRTHVEAFAALPSGHVFVAGMHCDSPDGPSPGLGVEHWAPGAKQP
                     VYRALPDLPAEGAQGGLKALGIAALSASDVYLAAEGNYLAHFDGQQWSRQDCPGAGGF
                     IELYAVEGDTVFGLDSSRALWRRRAGEEWQGLPLISSLRKAALEPGVLREGAHLGEAK
                     SVEVRSFWPKTATDVWALAQLNSGGSGSAAGKRSFLLHTHEATGPLPTLDDIDRADRQ
                     ISVPGPPVSWCRTPFVLLYTLGRNAPADYDYPATRAALKGHTEFGEAEFIEFARNGRR
                     YFGARVADFELGRKLSELVGKRVPGSTPELVCHDPVGSRVLRIELETGKLRQ"
     gene            complement(11599..12669)
                     /locus_tag="VFQ61_19515"
     CDS             complement(11599..12669)
                     /locus_tag="VFQ61_19515"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET9956698.1"
                     /translation="MSDHSKALMLALLGACAPPAPASIEAIGSELPGPAPRGRESARA
                     QAMNISCVECHRAAAREWAVSRHAQAFTNPEYQRALAIEPLGFCRGCHAPEADPERPP
                     DAAAAALGVACVTCHVVGDEILAASRSESDGPRAPHGVVRDGRLDFARACVRCHEFEF
                     PDRGARGKSELMQATVSEQAGSTAARRACADCHMPASSTPGQSRRSHLFPGGHNEHLV
                     RSAVQTSATRVDAERVRISLTPQAIGHAFPTGDLFRRVEVTAEVVGDEWQVLEARQAY
                     LARHWTRTERGPFGVRLRTMARDDRPLDVPVALDWSFEMGSGRAIHWRVAYQRVAHPR
                     SESEQESEVTGEIELAHGVLSP"
     gene            complement(12800..14983)
                     /locus_tag="VFQ61_19520"
     CDS             complement(12800..14983)
                     /locus_tag="VFQ61_19520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9956699.1"
                     /translation="MRLTFRFILLITVLLTSVAASAVAGWRATNQLQQALQRVVEQDM
                     ECLLSITHTRRLFRSMVVLERDYILSSSKKERAGFATKMTKLSEELDQQLEKYTRRMP
                     AEDRGALVDIRGAKQRFLEVHTQVQRAAETSQEAALATAKLHSQDPVSWEKVIGGLVS
                     MSETRLSNQVKQTYAVHRTARHTLLAVSGLACLIAVSLGYAIFVGIRRNVQQVVALNT
                     NLEGQVELRTRALGQRERALRLVLDSTGDGILGVHADGTLASEPSAAAQQWFGPVTPG
                     ISAAQYLFPEDEEAQQLFRASFEQLLEDVLPWEVCVEQMPRRIRRGELVLDIEYRSIS
                     ANGAESNEARVLVIARDVTARERSERNEQGVREQQALIAKLLQDKLGFTQFVREAETL
                     IQTLSTTQDPRVARRALHTLKGNVAIYGLASVAKLCHSIEDRLEEHDGLPVAADLADL
                     GALFRAKLESIESFLSSVSKDVLEIRNEEHATLVESLVQRKDYQDILKLVEMWSWYRA
                     QERLMRLKAQTEYVAERLEKAVDIQVEHADVRLPPGYLERVWPTLVHVVRNAVDHGVE
                     SPEQRRASGKPEHAVIRLSTYQSVDNIVIEISDDGPGIDREALLRAAERRGLEITDQT
                     SIADLVFTEGLSSRDRVSEISGRGVGLSAVLEACRAEGGRVEIRNQSGQGTAFLFYFR
                     RPRIKTGGLAAQLEKNWSLTPSSLVEHDEPNSAVLRSGPALMSGS"
     gene            complement(15261..16118)
                     /locus_tag="VFQ61_19525"
     CDS             complement(15261..16118)
                     /locus_tag="VFQ61_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956700.1"
                     /translation="MDHSRALGREWLLLLAVVGGAGAALFGSGHRGVAAAASPNSASA
                     PSAPLIDDFAVSPIPGDSWTDGSDAAQPEEGDTVSGDVLEQIDVGQYSYLRLGEKGGI
                     DIWAAVSSAPSRLGKRTTVTNAQLMTNFTSSTLKRTFPSIYFGAIRDESAAPASGQGQ
                     SPRPEIASGMAHIPEVTVPRARGPAGYSVAELNGAAAAAGMKVRVRGIVVKSTSGVLG
                     RTFLHVQDGSGDVKSGNHDLAVTTTAEIPVHSEVELEGTLEVDRDFGAGYRYRVLLSD
                     AAIVPAHSH"
     gene            complement(16139..16708)
                     /locus_tag="VFQ61_19530"
     CDS             complement(16139..16708)
                     /locus_tag="VFQ61_19530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9956701.1"
                     /translation="MCANLETRDISRHPAGVAALYLEYAVFVRRTLRLHGLSGASVED
                     GLQDVFLVAVRKLDGFIPRASHKTWLYAISARVAKEHRRRTKRKGGLLELEQMELCSP
                     GHDPYTALATARVLRAVEERLSLLDDNRRKVFVLAEIEGLTVPEIARTLHVKINTVYS
                     RLRAARRQFGGGLTVTPARGVVHGESADL"
     gene            complement(16717..17553)
                     /locus_tag="VFQ61_19535"
     CDS             complement(16717..17553)
                     /locus_tag="VFQ61_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956702.1"
                     /translation="MSPAGATGSGGTSASGGSASTAPSTGGASPTVSGGASSSPSGGA
                     SQPSGGGAPNPRGGATQVGGSGGALGPSGGTSAAGAGGSLGGNTGLPPQGGTPPSGGQ
                     PNTGGTPAQGGSTNGGSSQGGSSTGTGGTPTNTGGTSTATGGASASTFAWPAAYNASG
                     LPSPTNGRHNPGNGCMGSSCHATRSPFAFGGTVYKSGGTSGAPNVQVGIKNGGTVLTA
                     YSASNGNIWLPTSAGSINWDQAAIAVRNAKGERVKPAGAPRGADCNGSGCHDSGSRII
                     EP"
     gene            complement(17851..19506)
                     /locus_tag="VFQ61_19540"
     CDS             complement(17851..19506)
                     /locus_tag="VFQ61_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956703.1"
                     /translation="MIRGAGLLLILFGVGSVSPAQAQTVTRAIAVRVVDATDERAYVE
                     PGAADGLRVGDELAFGGRRLKVVSVSSSFAAVALLGRPLAVGARGQVNIVTNRPEETV
                     LTLAKPTPLSAFRSNRLRGVPPALTQQPTPVPLGTRTRSRNRLVFSDAFYALLPSQGR
                     SKMLANELRGRLHYESMTRSPWGLDADVAAQTFAGDDFSLRPGAASRQVLRVRELSLT
                     YGTATDFRASLGRLRAASALVGQLDGVRLEAPLLPGVRVSAFGGTLPHAFNGMVSTRV
                     ARFGGELAFQAPAAPWQPRLMLGGHASHFDGALDERKVYASFDLLPEKARLGGHAEVS
                     FFDANNPWHAHRIELTSATLDGNFEVDIFHFGGRVDFRRPERSRWLASLLPIEWLCWS
                     DLARAGNDCRSNPQIVSWTADAGVRLGRYALDLGGHSSYTRGTDAATFGGFANLRWLG
                     ERLHWDAGMTAVSGSVMRSVALALIPGASIMQGNADLSVRYRPAVIRYRTDPRSRVEH
                     SIGTALWVAPSQRFEAEILADWMQAADVTLLVAQVVGTWRIDP"
     gene            complement(19503..20378)
                     /locus_tag="VFQ61_19545"
     CDS             complement(19503..20378)
                     /locus_tag="VFQ61_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956704.1"
                     /translation="MKHARIAAWLACGLISAAGFRAVAEPGRHVSITEGLDCSSCHSQ
                     SSWSIAHKTAGQSGFDHSRTGFPLTGQHRAGNCVDCHRADRAITRECVGCHADAHQRQ
                     LGQACDRCHTASSWAAVSGIRLHRNTRLPLSGMHTLAACSECHVRASENQWRGAPADC
                     YACHAGDYQRGDIHPLHRGVAGDVSKPALPKNCGSCHRTLSWSPAFAPATFTFRSTGQ
                     PLVALQHDAVFPISFGKHRRAACGDCHAQSEAPRLVVCTSCHAHSLPVLIAQHRGTAP
                     VISGCLHCHPGGARR"
     gene            complement(20375..>21646)
                     /locus_tag="VFQ61_19550"
     CDS             complement(20375..>21646)
                     /locus_tag="VFQ61_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956705.1"
                     /translation="VKVECSACHRGSPPKYKGLEFSSCTSCHNDPHDGKLGPACESCH
                     LESDWHQVTVGVRNHPWLSLANGHARTPCIKCHDRGTLRTPSRGRACAGCHKPVHEAN
                     FGKRCESCHEVILWLGLARSVGLNAHGKTVYPLRGKHLESPCAGCHKPSLPEAERYRQ
                     LEFDRCSRCHKDVHDARFAKRDDGECKACHSESGFRPSRFGVTLHHSTRFPLEGRHTA
                     VACGKCHQHDPKRRERLDWSSKKLRCAECHENPHGDQFQAEMRRNGCATCHTPASWTL
                     PRIDHSVWPLTGAHASTPCAACHTPTEQDRKAGAGPSYRLAPRACDGCHDDVHRGQFR
                     LAEPKRACEFCHSTVEFKVPDFDHTRLTGYELLGKHKALKCDACHTHKPTPGGGKAAL
                     FRLGYRRCRDCHADPHAERKGTLASSSGGLP"
BASE COUNT         3948 a         7038 c         6770 g         3890 t
ORIGIN      
        1 cgtattgacg agactagaac gtcgccaggc cggccgctcg ctgcccagca tgggtaagta
       61 cgcgcgccgc gctccccacg ctaggcttcg gcccgaaggg gtctcctcgg cacttgatct
      121 gggcgccttc gacagtctca taatgattcc atggccaagg cgcacacgga gtggacggtc
      181 cttccccacg aatccctgga aaagctcgcg gacaacctgt ggtgggtccg gggctccctc
      241 aagggaatgt ctctgaagcg gaccatggtc atcgcacgct tggccgatgg acgactggtg
      301 attcacaacg gtatcgcgct ggacgaaccg agcatgaagc aactagaggc ttgggggaat
      361 cccagcttct tggtggtgcc gagcggagtt caccgcttgg acgcgccagc cttcaagaaa
      421 cgttatccgg ggctccgtgt gatcgctccg cgcggtgccc gcgcccagat cgagcaggtt
      481 ttgccagttg acctgaacta cgacgagttt ccggccgatg atgcggtgcg attcgagaac
      541 ctccagggag tcggcaattc cgaaggagtg atgatcgtga attcatctga cggcgctagc
      601 gtggtgctga acgacgtggt cttcaacatg gatcgcaagc gcgacttgct gggattcctg
      661 tttacgaccg tcatgggctc ggccccgggc ccgcgcgtct cacggctcgg gaagctgcta
      721 ctggtgaagg acaaggcggc gctgcgcgcc gatctcgagc gcctcgccgc gctgcccaac
      781 ctgcagcgcc tgatcgtggc gcacgaaaag gtggcgagtg gacccgaggc gtccgacgcg
      841 cttcggcggg cggctacata tctctagccc gggtgtgcca agctgatccg gcatcggttg
      901 cgcttcacga ccgccgctcg ggtgcctgca ggacgagcga taagccccct ttcgggtgca
      961 acgagatctg tgcctgtagc tcgaccggag atccggggtc gcttgggtga aggctgaatt
     1021 cgcgcagcag cacggcgagc gtccccacgg cttcgagcag cgcgaagctc cgcccaatgc
     1081 aggcgcgagg gccgaggcca aacggcaagt atgctccttt ggggatttcg ctttcacgca
     1141 cgaaacgagc aggccgaaac acctcggggc cgtcgaacca acgagcgtct cgttgagtga
     1201 tatagggaac gatcgtgaca taggtccccg cgccaagcgc tacgcctccg atggttacgg
     1261 gctcggcgac ctcgcgggga atggcgtaga ttggcggata gaggcgaatg gcttccttga
     1321 cgacgtgctc gatcagtgaa agtcgactca agtgctctgg ccccacttcg cccgagccaa
     1381 tgacgcatag caactcggcg cgagccgctg cttgaacctc ggggtgctcg ctcaagcaat
     1441 acaacgacca aatgaggctt acggccgtgg tttcgtgagc gcccaacagg acattcacgc
     1501 tcgcatcgta cagctcgcga tccgtgagcc gactgccatc ctcgtctcgt gagtctaaaa
     1561 gaaccgacag tagcgtaggg tgttctgggc gttgccggcg cgcgctcgcg atgaatccga
     1621 acacgactct ttcgaggtag gcagctgact ctcggactcg gcgtttgaga ggcgtcgggg
     1681 cccatttcgg caaccacacc aatgaattca gctcacgaat agcgcactcg ttcaggttgc
     1741 cgacgtgccc gatgaactcg tctagatgcg tatcgatctc cgtgccaaac atggcctccg
     1801 ccgctacggc gaaggtcaaa cggctcactt ctctggacgc atcgaaggat gggctcacct
     1861 tcgcccggct gaccatttcc ctcgctcgac gcaggatgat ggctgcgtgg atcggcccga
     1921 gttggctttg gaaggcaggc ccaacgagcc gccgtacgcg cacccagcgt tcaccgtcga
     1981 tgagggccag cccgtcgccg ctccattggg acaggacgcg ccgcacgcgc tgtgacttgc
     2041 ggaacgcgcg gtgcttctcg acgagcactt gctgaatgtg ctcgggatga gtgacctgag
     2101 cgaagggctg gcccgccagc gcacaagtca ctaagtcgcc ttgttctgcg agacgcgaga
     2161 agaacgcgac tgggtctcgt tggagaagat tcaccagttc cagcccggca aaaccagcgc
     2221 ggacgtgccc cacccgcggg atcacccgag cacgctctac gttgctttgc tgcatcacgg
     2281 gcatgttgtt ccggcaaggt tggccgagcc agagcccgac tcacttgctc acgctgtgag
     2341 cacggcggga gttgccgcgc gctgggcaga cgctatgaag cagtcgtgcc caggtcgctc
     2401 agaaacgctc agcgcaaagc cgaggcggcg cgactgcgag agctgcgcgg ggtattggga
     2461 ttgacgcaac gcgagctcgc caccgagttc aatgtggctc acggcgcgat cgccgcatgg
     2521 gagaacggca atcgaaccct acccgggccg gtactcaagt tgatgagttt gtacgaggag
     2581 gagctcggct tggcgcctcg agccgcgacc ctcgagcggc ttcgaacgtc tcgctgggcc
     2641 agacaagcgg ccctctcgcg cacagccgga gggatcctgg cgcgggttgg ggctacggcg
     2701 ctcgaacgca tgctggccga cgacgtgcac gcaaacgcca tcactgcgcg cgcgcacacg
     2761 gccgttgcac gcagtctggt cgatgcgctc ggcaacctaa aaggggttgc cctgaagttc
     2821 gggcaggccc tgagctacct ggattttctg ctgccggagg agtcccgccg ggagctccaa
     2881 accttgctgg tcacgagccg cgcaatgtcg gcctcggtca tctcacaggt ggtgatggag
     2941 gaactcggcg aaccaccgcg caggctgttt tcggagtggt gcgcgcagcc ttttgccagt
     3001 ggctccatcg gccaggtgca tcgcgcgcgt ttgcgaagcg ggcaggaggt cgcggtgaaa
     3061 atccagtacc ccgcaatcgt gcaagcgatt caggcggact tggcggcggc ctcgctcatg
     3121 gatcggatga cctccctact gttccgtgcg cagcaaccgg gagtggtgct ggacgagctc
     3181 agggagcgtt ttctcgagga gtgcgattat cgaatcgagg ccgagaacca agagcagttc
     3241 cgaaccctgt ggcggggcca cccaaaggtc catatcccgc gcgtgcaccg cgaactctcg
     3301 acctcacgtg tactcgtgac cgacttcgcc cacggcgaga acttcgagtc cttcctgcag
     3361 catgcgtccc ccgccgaacg agactgcgca ggcatgcttc tgtggcgctt cgcgttcgaa
     3421 tccatcttcc gccatggact tttcaatgcc gatcctcatc ccgggaacta tctcttcgcc
     3481 cagggccggg tcgtgttttt ggacttcggt tgcgtgaagc gcttcgaccc ccgcatgctg
     3541 ggcctttggc gagggatgat tcgaggggtt ctggaggggg acgccgaaac ggcgcaccgg
     3601 ctgtggatgg aagctggaat gatcccggat ccttcgcgct tcgacgccga gtatcatcga
     3661 catatgcttc tcactttgta cgagccgtgg ctcggcgcgg agccataccg cttcgagccc
     3721 gagttcgtag agcgcacttg gcgcgcgctc gtgctcgaca accccaaccg ttttcgcacg
     3781 aacctcccca aagactgggt tttcgtgaat cgacttcaat ggggcctcta tgcggttctc
     3841 gcgcgactcg gcgccaaggg cgccttcaga aacgacttgc tcgatctgct ctattcgccg
     3901 cacgagcgcc ggccaaatcc cttgacgtat accgctcggt tgggctgacg cagagctccg
     3961 ttgctagcca aaccgtctgt ctgtgccata ccgttgcgca cgctatgagc gcacggaacc
     4021 acctcggcga ttctcacaat ttcggacggc gggtgacgct aaaaggccaa atcgtgagta
     4081 agccacggtc ggtgttttgg gagcaattga tattggacgg gcagagtcca gttcgtcagt
     4141 tcctgaacgc cgcggcagag cacgatggca ttggctcgaa cgcttttgct tttcttccga
     4201 acctgcgttt cgcccctact ccggagggtg gcgatgtcga gcgcatcgag ctggcgccgc
     4261 tgccagcgct cacggccgag cgccaacacg agctcggacg gatcgtaggc cgctccttgg
     4321 cgctttggag ctggctcggg gtgagcgacc tgcactggga gaatctagtc ttgggggtgg
     4381 atgcaggagg gcacatcagc ttcacgccgc tcgacatcga gatgatgctc gcagatttgg
     4441 cgctgccgac cgagacgaag ctgttgccgg acgcagatcc ggagtacgcg gagacttcgc
     4501 gccatgcctg cggcatgcgc cgcgttctgc cgtacctggg caagcccatc gaggccagcg
     4561 cattgctcga gatcttgggg gcataccacg ggacgttgct gtttctcgag cggcatgggc
     4621 gtgacatcgg gaagctgatc gcggccttgc caggcctgag ggaggcgccc atgcgcgtat
     4681 tgttgcgtgg cacggacgaa tacgtgaatg cccacactcg cgaactttgg ccccctctgc
     4741 tcgaagccga agtcgagcaa ctccggcgcg gagatatccc ctacttcttc cgtctgtatg
     4801 gtcgccccgg aatccactac tatgccgagc caacgctgca aacctttcga accctgccgc
     4861 tggagggcga catgcctcag ctcgaaccgc tgctatcacc gtctcgaggg ctgagctcgg
     4921 gctcgcgcaa gcggctgagg gaggaggggc ttttcaccgt gctcggagca ttcgatcatc
     4981 cctcattcgg cggtaagcac ggcaacggta atctcgagct cgttctttcc aagcgtaagg
     5041 tgatcctaac gttgccgagc ggtgaagaga tccacgctcg gcgggatttg cgggccttcg
     5101 tgagctcggt ctacttgccg tgccgatgtg gcgaggtgcg aacgccgttc gttccgcgat
     5161 taacacgctg tgaggcttga ccgcggcatg cgcccgcgtg ccgcgggcgt gcattgcgcg
     5221 cgggggcgcc tatagtcacg ccaccgtgac cgtccctgcc tgtgaagtct gttctcgccc
     5281 caaagtagcg tgtgtctgtg accgggtcgt ccagtacgag acccagcgtc gagtgctcat
     5341 cctgcagcat cctcaagaac aggacgcttt gcttggctcg gcgcagctct tggtggcctg
     5401 cctaccgaag gtgaagctcg tggtggggct cagctggcgg aatctagctc acgcactcgg
     5461 ggaagagagc gtcgatccca aacgttgggc cgtgctcttt ccggatcgtg actcgcccgg
     5521 caactgtgtc accggtcggg gggggccaat cgatcccgcg cagctcgagg gcattatcgc
     5581 gttggacggg acgtggtcca aggccaagac gctctggtgg cgcaatccat ggctgaacaa
     5641 actccaccgg ctgacgctca cgccttcgaa gccgtcgatt tacggacgca tgcgggttga
     5701 accgcgccgt gagttcgttt ctaccctcga atcggtgtcg gcggcgttga ccttgtgcgg
     5761 tgagccaccc gagattgaaa ccggcctgtc gcgcatcttt cgtaccctgg tgcagcgcgt
     5821 gcgcgacgca aacatcattc cccgctgata acgcgagcac ccgaagggct ttgaataggg
     5881 cgctatcatt gccgagctac tggcggcggt ggttctagcg cgtctccgga gattcgagct
     5941 cgatctcatc cacctcgagc gtgctttcct ctttcttctt ccgacctgga ggaggtggcg
     6001 gcatgcgccg gggcggcagc agtggcgcga ggtgccgact actcagctcg agcgtgcgtt
     6061 ccgatggctt ctccgtcggg gagtctgcta gcgagctgat ggagacggct tcgagctcag
     6121 cgctcgtaat ggcttgggac ggagcgctcg ccgggctgga ctccggcgct gtgctcgtct
     6181 ggtccgtctt ttcaggagct tccgcttggg cgttcacgtt gcctcgctga ggcggggccg
     6241 gcgcggagct ggcttcagcg ggcgcgatat gtagcgctct tttggcggcc gcccaaaacg
     6301 ccgcacgctg actgcgccgc cgtgcggctt tgacttcgaa gacgtcgatg aacgcgttcc
     6361 agagcgctcg cacgttgcgg aatcgctggt ccggatcgat cgccaaggca gtggccaccc
     6421 actcgtccgc gtcccgaggt agatccgggc ggtgcgcgaa caaactggga cgagcgctct
     6481 gggtcgcgcc catgaacttc tgctggagcg aagtcccggt gaacgggagt tcgcccgcga
     6541 gcacgcgaaa taagatgact ccgagcgaat aaacgtcgac ccgctgatcg atccgctccg
     6601 attgaccctg ccaggactcc ggcgccatga aactcgggga gcccatgcag gtgccggcgg
     6661 cggtcagcgg cgcggcccga cgcagacgtg ccatgccgaa gtccagcaaa cgtacgctgc
     6721 cgtcatcgag caagaagatg ttggctggct tgagatcgcg atgcaggatg cccgcgccgt
     6781 gcgcgacctc caacgtctct acgattggct ccagccgatc ggcgacccag ctcggttgga
     6841 tgcgctcatg gcgatcctcc acgctgtaca gatggttctc gagatcggtc ccggccagaa
     6901 gctccatgac caagcagagc tcgccgccgt tgctccgaca gatgtcgagc acttccacgg
     6961 cggccgttcc ttttagggca ctgagcgccc gttgttcgcg caccaagcgc tcggaaacgt
     7021 gcgggtcacg cgcggcgttc gaatcgagca ctttgatggc aacttcgcgc tgcatccagc
     7081 ggtctcgtgc gcgatacacg gtgccttgcc cgccgcgccc gagcacaccg agccgctcgt
     7141 aacgctcggt gatgttgcga tctatcgctt tggccacgcc caacgctatc acgcgagcgc
     7201 gtccgcggcc gctcgcggcc acgggcgcag cataaatacc aaagcgaaac atgcgaacta
     7261 cgcgacaaaa cagcgatctc cggttcggga caccgcccgt tggaagatcc gccacgttcg
     7321 agtcgtatca cggcaatctc tcgaatccgg ctcctctcga cttccgcgcc gcgctcggcc
     7381 ggtttcgcac tggacacgtg aacacaagct gcgggagccg cggcgcagcc ttatcagccg
     7441 gctcccgaac gacacaacgc tgctcgacgg gcgggacccc acctgaatga gatggcgtcg
     7501 agcgagcgtt gtgagcgaac gcatgatgcg cgttactcgc tcgtcgccgt cagagtttta
     7561 cagtttcctc atcctgcacg agaaacgtgt gcttggaggt tgtttactca cgcgagcgct
     7621 gtactgtgtc gagcgcatgc aaaaaccgac gatcgccgct ctgttgatgc tcgggtgtag
     7681 cgcagctttc cttggatgca gcgcagaggc ggagaagaag ctcgctgaat acaagaaaga
     7741 gtcggatgag cggatcgcca aaatcgaaag cgatgccaaa tccaagctcg cggcggccga
     7801 gcagaagctc tccgacactc agcgccaata cgaggagctg aagacacagc tgacggaagc
     7861 caagagcaag accgaggacg cagagaaagc caaggcgagc gccgaagaac aagccaagct
     7921 cgccgaagca gccatttcga aagcgcgcca ggccttcaag gaaaaggcca aactccagct
     7981 agccgacttg aacaaggagc tcacggacgc aggcgtcaaa gccagcaagg cccccgccaa
     8041 ggccaagact gctttcgcga ctgcagtgca ggacgtaaag aagcagcagg cggccgtggc
     8101 caaggacatc gctgcgttcg acacggccac gctcgaaacg ttcaagtcga tcaacgcccg
     8161 catccaaacg gatctcgcgg tgttgaagaa caagatccgg gcagcgcgag ccaagttgcc
     8221 cgcgagttga agtggtggtt cgcggcctta cgccgccgag gcgcgcacgg gcagagccac
     8281 tggtactttt ccaggccgct cgagctctcc gaggttgcat cgcccgcccg ccagcatcgc
     8341 ccgcccgcat cgcccgccgg cgaaagattc tggggggcat gctcgaacct gccgaatgag
     8401 agaaggatgc gagcatgccc cccggggttt accagtcggc tacacacatc gcgctgggcg
     8461 cgcgcctccc gatcaacttt ccccgtaccg acgagtagaa atctaactaa aagagccctg
     8521 cgtgccattg ttgcctggtg ccaaaccgtt gtggtttcgc gccaggagcg cccgttggcc
     8581 gaacaaccgc cggcaccggc tcttgggcac tggttctgag gacgggttac gcgaccggcc
     8641 cacggcagca gccgacccgc gtcgaactag ctcctattgg gatgggctct tagcctcccc
     8701 tgccccactt cggcatagtt cgcacccagc gcctgccgtc tgaactcccc gggagtagtt
     8761 cccgtccagc gcacgaaagc atggtggaac gccggagcgt cgttgaagcc gagcgcaccc
     8821 gcgagttcgg agatcgcgag ttctcggcgg ctcaggagcc gttcggccgc cgcgcggcgc
     8881 attcgcgctg cgatctccgc gtacgtcgtg ccctcgagtt ggagagaacg ttgaagggtg
     8941 cgcgctgcca cgccgagctg ctgagcaact tgagcgatcc ctggatcgat gcctgcgcgc
     9001 agtacgtgtt ccactctctg agtttgcgtt tcggccggag tcagactctt catcaccaga
     9061 tcggcataac gagtcagaat atccgacaat gcgtcgtccc tctgccgcat gggagcctcg
     9121 agcagtcgcg cgtcgaacac gagttgatcc tgcgccgctt cgaatcgcgg cggtacgccg
     9181 aacaactcgg tgtgagtgcc gattcgcgcg ggtgcctcgt gacggaagta ggccgcgcgc
     9241 aacccccaat cttcggggcc gaaacgctga aagaaaactt gtcgcgtacc caacaagaac
     9301 tccgctactg gcgccagcgg cggtcgagcg gggcgctcgt gcagcgtgat tgctgcctcg
     9361 ccttcgctta catccagggt ccactcgatg gcattggtaa tcaagggcag gtattcgaac
     9421 tttttgcgca gagcttcgcc caccgtcctg gcattcccct ccaagtaggt aatcacgtcg
     9481 caaaccgtca gatcaacgtg ctgcgcggcg tgcaacgcga actccggatc ctgcgtcacc
     9541 tccaggagtc gcccccaaac ccccgcaatc tgaggtaaag gtagcctcac gtcccaaccc
     9601 gtgaccgccg gatcaggcat gccgaattcg gcaaagaatg ggcccgctgc taccccgcgc
     9661 gattcgagag ccaaagcgac cgggcgatat agacgaacgg cgaccgtcgg gatgaacatt
     9721 cggcaaacct tgagcgcact ctgcctcagc ggcgttgccg cggcaaggcc agcaagctcc
     9781 gggtagcttc ggcgttattt tgcagctatt ggcgcagctt gcctgtttcg agctcgattc
     9841 gcagcacccg agagccaact gggtcatgac acacgagttc cggagtagag ccgggtactc
     9901 gtttgcccac cagctcactc agctttcgtc cgagttcgaa atcagcaact cgcgccccaa
     9961 agtagcgccg gccattgcgt gcgaactcga tgaactccgc ttcaccaaat tctgtgtgac
    10021 ccttcagcgc agctcgggtt gcgggatagt catagtcggc gggggcgttt ctaccgagcg
    10081 tatagagcaa cacgaaggga gtgcgacacc aactaacggg cggaccgggg acgctgatct
    10141 gtcggtccgc acgatcgatg tcgtcgagcg tcgggagcgg accggtggcc tcgtgggtat
    10201 ggagcaagaa cgatcttttg ccggctgcgc tgcccgaacc acccgagttc aactgcgcta
    10261 gagcccacac gtcagtcgct gtcttgggcc agaaggaccg cacctcgacg ctcttcgcct
    10321 ctccgaggtg cgcgccttcg cggagaaccc ctggttcgag agcggctttg cgaagagaag
    10381 agatcaacgg gagcccctgc cactcctcac ctgctcggcg gcgccagagt gcgcgactcg
    10441 agtccagacc aaacaccgtg tctccttcta ccgcgtacaa ctcgatgaag ccaccggcgc
    10501 ctgggcaatc ctggcggctc cactgctgcc catcgaagtg agccaggtag ttcccttccg
    10561 cggccaggta gacgtcactt gcggaaagcg ccgcaatccc gagcgctttg agtcccccct
    10621 gcgcgccctc ggcgggcaag tctggaagcg ctcggtacac gggctgcttc gccccgggtg
    10681 cccaatgctc gaccccgagc cccggtgatg ggccatccgg agaatcgcag tgcatgcccg
    10741 cgacgaaaac atgtccggag gggagggccg caaacgcctc cacgtgggtg cgcttttgac
    10801 agaacccttc gggagacgca ggtctacgga actccggcgc gccgccctca tgcggaacaa
    10861 gccgagccgc tgcaactcca ggcaacaccc agaaggcggc atcgaacaga tacgagttct
    10921 ctagaactgc cagcatccga cctgaactcc acggctggat atcgaagacg aattgcgcct
    10981 cgttcgactt tcgttcgcgc acccagcgcg atcctctcca gcgccagagt tgactgtaac
    11041 cactgcggcc ccacggatgg ctcgtggaca gccaaaccgc gtctggccag cgccccacta
    11101 tgccgttcag tgagtacatc aaatcgtgtt gcagctccgg caccacgcgc gcctcgtgcc
    11161 gcacgtcatt gccgacgagg tcgagcagaa tcttggcttc gtccgtgacg aaaccgcgct
    11221 caccggcgcc gtagaggtgc agattgttcg gaacctcgcc gattacgtga aatgggagtg
    11281 gcgtcgattc ctcgggctgc gcgcggggct ccgtcgttcc tgccctgggt tcatttggag
    11341 acgcatcggc ggtgctgctc cgagcgtgca ccgccgccgg cgcacgcgcg tcgggttgtc
    11401 gccgggatgc ccaatggtcc gaaggcgtgc acgcggcact cgaaacgaag ctgagaaaaa
    11461 cgacttcgac gagtggcgtg cctcccatcc tccaccgtct cgtacccagc gtgccgcttg
    11521 ggctcgttcg caccgtcgct gcgatcatgg tcgagccatc ttgctcgttt gcgctccgaa
    11581 atcaacgacg ctgaagccct agggcgacag tacgccgtgg gcgagctcga tctcaccagt
    11641 gacttcactt tcctgctcgc tctcggagcg ggggtgggcg acgcgctggt aagccacgcg
    11701 ccaatgaatg gcccgaccgg agcccatctc gaatgaccag tccagcgcga cgggtacgtc
    11761 gagaggacga tcatcgcgcg ccatcgtgcg cagtcgaacg ccaaagggtc ctcgttcggt
    11821 acgcgtccag tgccgggcca agtacgcttg tctcgcctcc agcacctgcc actcgtcccc
    11881 caccacttcg gccgtcactt ccactcgtct gaagaggtcg ccggtcggga atgcgtggcc
    11941 aatggcctgg ggtgtcaggg agatccgaac tcgctctgca tccacccgtg tagcgctagt
    12001 ctggaccgcg gatcgcacca gatgctcgtt gtgaccgccg gggaataggt ggctccgacg
    12061 tgactggccc ggagtgctcg acgccggcat atgacagtcc gcgcacgcgc gccgagcggc
    12121 agtggacccc gcctgctcgc tgaccgttgc ctgcatcagc tcggatttgc cgcgcgcgcc
    12181 acgatctgga aactcgaatt catggcaccg cacgcaagct cgcgcgaagt ccaaacggcc
    12241 gtcgcgcacg acaccatggg gtgcccgcgg tccgtccgat tccgagcgag acgccgcgag
    12301 gatctcgtcg ccgaccacat ggcaggtcac gcaggccacg ccgagtgctg ccgcggcggc
    12361 gtctggcggt cgctcggggt ccgcctccgg cgcgtgacag ccgcgacaaa atccgagcgg
    12421 ctctatcgcc agcgcacgtt gatattcagg gttggtaaag gcctgcgcat gccgcgagac
    12481 cgcccactca cgagcggccg cgcggtgaca ctcgacgcac gagatgttca tggcctgcgc
    12541 ccgcgcggac tcacgaccgc ggggagcggg gcctggaagt tccgaaccaa tagcctcgat
    12601 gcttgctggc gcgggaggcg cgcacgcccc aagcagggcg agcatcagtg ctttcgaatg
    12661 atcactcacg cgcgtctcgg atatcagcaa ccgtctagcc aggggccatg atatctcgat
    12721 ttcgcccgct gtcacacggc gtgctccccg cgacccgcat cagccggccg tgcgtactca
    12781 gctcgccaac tacgaggcct cagctcccgg acataagagc ggggccgctc cgcagcacgg
    12841 cggagttcgg ctcatcgtgc tcgacgagac tgctcggagt caggctccag ttcttctcga
    12901 gctgagccgc caagcccccc gtcttgatgc gcggccgccg gaaatagaag agaaacgcgg
    12961 tgccttggcc gctctgattg cgaatctcta cccgcccgcc ttccgcccga caagcctcga
    13021 gaaccgccga caacccgacg ccccgacctg atatctcact cacccgatcg cgggatgaca
    13081 agccctccgt aaacactaga tcggcaatcg aagtctggtc ggtgatttcg agacctcgcc
    13141 gctctgcggc gcgaagcaaa gcctcgcggt cgatgcccgg gccatcgtcg ctgatttcga
    13201 tcacgatatt gtcgacggat tgataggtgg agagccgaat cacagcgtgt tccggcttgc
    13261 cgctggcccg ccgctgctcg ggcgactcca cgccatggtc gaccgcattc cgcaccacgt
    13321 gcacgagggt tggccagacc cgttcgaggt aacccggagg caagcgcacg tctgcgtgct
    13381 cgacctggat gtccacggcc ttctcgagcc gctctgcaac gtactccgtc tgcgccttca
    13441 gccgcatcag gcgctcttgg gctcgatacc aactccacat ttcgacgagt ttcaggatgt
    13501 cttggtagtc tttgcgctgc accaagctct ccaccagcgt ggcatgctct tcgttgcgaa
    13561 tctcgagcac gtctttgctc acgctgctca aaaaggactc gatactctcg agcttggccc
    13621 gaaacagagc accgagatcg gccaagtccg ccgcgacggg cagcccgtcg tgctcctcca
    13681 ggcggtcttc gatgctgtgg caaagcttgg caacggaggc gagaccatag atcgctacgt
    13741 tccccttcag ggtgtgaagc gcgcgccggg cgacgcgggg atcctgagtg gtcgaaagcg
    13801 tctgaatcag agtctccgct tcccgaacga actgcgtgaa tccgagtttg tcctggagca
    13861 gcttcgcaat caaagcttgc tgctcgcgca cgccttgttc gttgcgttcg gagcgctcgc
    13921 gcgcggtaac gtcccgcgcg atgacgagca cacgtgcttc gttgctctcc gcaccgttgg
    13981 cgctgatcga gcggtactcg atatccagca ccaactcacc ccgcctgatc cgtcgtggca
    14041 tttgttcgac gcaaacctcc cacggcagca cgtcctccag caactgttcg aacgatgcgc
    14101 ggaacagctg ttgagcctcc tcatcctccg gaaagaggta ctgcgcggcc gagattccgg
    14161 gcgttaccgg tccgaaccac tgctgcgccg ccgccgaagg ctcgctcgcg agcgtgccgt
    14221 ccgcgtgcac tccgagtatg ccgtcgcccg tgctgtccaa cactaggcgc aacgcacgct
    14281 ctcgttggcc gagcgcgcga gtgcgaagct ccacctgccc ttccaggttg gtgttcagcg
    14341 cgaccacttg ctggacgttg cgacgaatac cgacaaagat tgcatatccg aggctgaccg
    14401 cgatcaagca ggctaggccg gagacggcca gcagcgtatg tcgcgccgtc cggtgcacgg
    14461 cgtaggtttg cttcacctga ttcgataggc gcgtctcact catcgacacc aggccgccga
    14521 tcaccttctc ccacgatacc ggatcctggc tatgtagctt ggccgtggcg agcgcagctt
    14581 cctgactcgt ctcggccgcg cgctggacct gagtgtggac ttccaagaag cgctgtttgg
    14641 cgccgcggat atcgaccagc gccccgcgat cttcagccgg catgcgcctc gtgtacttct
    14701 cgagttgctg gtccagctcc tcgctgagtt tggtcatctt ggtcgcgaac ccggcccgct
    14761 ccttcttgga gctcgaaagg atgtagtccc gctccagaac caccatggat cggaacaaac
    14821 gccgagtgtg ggtgatcgag agcagacact ccatgtcctg ttcgacgacg cgctgcagcg
    14881 cctgctgcag ctggttagtt gcgcgccaac ccgcgaccgc gcttgcggct acgctcgtga
    14941 gcaaaacggt aatgagcagt atgaaacgaa acgtgagcct cactcgcatt ctccatcaac
    15001 gacgcggtcg cctcccagaa ttcggcactc tgcgccaacg ccagaccgag tagcggaccg
    15061 ttggcgtgtg gcataagggc cacagcggaa ctcggcccca ccgctcactt accaggcgtc
    15121 ccgcgcctat ctgaaacagc tcgcccgaac ccgcgatcta tcgcttcgtg tagggcgacc
    15181 gagcaggaaa tgcgggtccc gtgcacgccc gctttgggag cccctgatgc gcgtcacggg
    15241 ctcctgtcgg actaggcacg tcaatgcgaa tgcgcgggca cgatggctgc gtcgctgagc
    15301 aagactcgat atcggtagcc cgccccgaaa tcgcgatcca cctccagtgt tccctcgagc
    15361 tcgacctcgc tgtgcacggg gatttcggcg gtggtggtca ccgcgagatc gtgatttccc
    15421 gacttgacgt ctccgctacc atcttggaca tggagaaatg tgcgccccag cacgcccgag
    15481 gtgctcttga ccacaatccc tcgtactcgc accttcatcc cggccgcggc cgctgctccg
    15541 ttgagctccg cgacactgta cccggcgggc ccgcgcgcgc gcggcactgt cacctccggg
    15601 atatgagcca taccgctcgc aatctctgga cgcggcgatt gaccttgccc cgacgcagga
    15661 gcagcggatt cgtccctgat ggcaccaaag tagatgctgg ggaaagtgcg cttcagagtc
    15721 gagctggtga agttggtcat gagctgagcg ttcgtgaccg tggtgcgctt cccgagccgc
    15781 gagggagcac tggaaacggc ggcccagata tcaatgccgc ccttttctcc gagcctcagg
    15841 taagagtact ggcccacgtc gatttgttcg agcacgtcgc cagataccgt gtctccctcc
    15901 tcaggctgag ccgcatccga accgtccgtc cacgaatcgc cgggaatcgg cgacacagcg
    15961 aaatcatcaa tcagcggagc gcttggcgca ctagccgaat tcggtgacgc cgccgccgcg
    16021 acgccgcggt gaccagagcc gaaaagcgct gcacccgcgc cgccgactac cgcgagcagc
    16081 aagagccatt cgcgccccaa tgctctggaa tggtccatct cgttcagctc cgttgtcttc
    16141 agagatctgc gctttcgcca tgaaccacgc cccttgcagg ggtgaccgtt agcccacccc
    16201 cgaactgacg acgagcggcg cgtaaccgcg agtacacggt gttgattttc acgtgcagcg
    16261 tgcgcgcgat ctcgggcacg gtcagtcctt cgatttccgc gagcacgaac acctttcggc
    16321 gattatcgtc gagcaggctc aagcgctcct ctaccgcgcg gagcacccga gccgtggcga
    16381 gcgcggtata gggatcatgc cccgggctgc atagttccat ctgctccagc tccagcaaac
    16441 cgcccttccg tttggtacga cgccggtgct ccttggcaac gcgcgcggag atcgcgtata
    16501 gccaggtctt gtgtgaagcc cgtgggatga atccgtcgag ctttcgcacc gccaccaaga
    16561 agacgtcctg taagccgtct tccacgctgg cgccgctcag gccgtgcagg cgcaacgtgc
    16621 gtcgaacgaa gacggcatac tctagataga gcgctgccac gcccgctggg tgacgggata
    16681 tgtcgcgtgt ctccaggttc gcgcacatgg ggagcctcag ggttctatga ttcgactgcc
    16741 gctatcgtgg catcccgaac cgttgcagtc ggcgccgcgc ggcgcgccgg ctggcttcac
    16801 ccgctcgcct ttggcattgc ggaccgcaat tgcggcctga tcccagttga tcgatccggc
    16861 gctggtgggc agccatatgt tgccgttgct ggccgaatag gcggttagca ccgtaccgcc
    16921 gttcttgatc ccaacttgaa cgttcggtgc gccggaggtg ccgcccgact tgtacaccgt
    16981 gccgccgaac gcgaagggcg atcgcgtcgc atgacacgaa gagcccatgc aaccgtttcc
    17041 ggggttgtgc ctcccgttcg tgggcgatgg taagccgctc gcgttgtagg cagctggcca
    17101 tgcaaacgtc gacgctgagg caccgccggt cgcagtcgac gtaccccccg tgttggtagg
    17161 ggttccgcca gtgcctgtcg agctacctcc ctgactcgag ccgccatttg tagaaccacc
    17221 ctgggctggg gtgccccccg tgttgggttg gcctccactc ggcggcgtgc cgccctgtgg
    17281 gggaagcccg gtattgccgc cgagcgaacc gccagccccc gccgcgctcg tgccaccgct
    17341 tggtcccagg gcaccaccgc ttcctccgac ttgcgtggct cccccgcgcg ggttcggggc
    17401 gccgcctcct gacggctgag acgcacctcc gctcggggac gagctcgcgc cgcccgaaac
    17461 agtggggctc gcgcctcccg tactcggcgc ggtgctcgcg ctaccgcccg acgcgctggt
    17521 gcctccgcta ccggtcgcgc cagcgggaga cacggagtca gttccagcag tcgctcgggg
    17581 gtacccggaa tcggttccag aacaagccca gacgccggtc gaggccacaa tcggaaacag
    17641 gggagcgcgc aggcccagaa agatcctttg aagagcgatg tgcataccga gcaccttcct
    17701 tccaacgcgg tggcggttgg agtcgacgtt tgagtgcccc gaaccctgcg aatcaaggca
    17761 acgattcgtg agctcgatcg atctgcgcca gaaacgcgag actcgatgcg caaatgaaac
    17821 aagtcacgta gatccactct gggcgcgcgg tcacgggtca attctccagg tgccaacgac
    17881 ttgcgcaacc aagagggtga cgtctgccgc ctgcatccaa tcggctagta tctcggcctc
    17941 gaagcgctgg ctgggagcga cccagagggc ggtacctatc gaatgctcga cccgacttct
    18001 cggatcggtg cgatatctaa tcaccgccgg gcggtagcga acggataaat cggcgttgcc
    18061 ttgcatgatt gaggcaccag gaatcagagc cagcgccaca ctccgcatca ccgacccact
    18121 gacggcagtc atgcccgcat cccaatgcaa gcgctcgccg agccagcgca ggttggcgaa
    18181 accgccgaag gtcgcggcgt ccgttccgcg cgtgtagctg ctgtggccgc ccagatcgag
    18241 cgcgtatcgg ccgagccgca cgccagcgtc ggctgtccaa gacacgatct ggggatttga
    18301 tcggcagtcg ttgcccgcgc gcgccagatc cgaccaacac agccactcta tcggcaagag
    18361 ggaagcgagc cagcgcgacc gctccggtcg gcgaaaatcc acgcgtcccc cgaaatggaa
    18421 tatgtcgacc tcgaagttcc cgtcgagggt ggcagaggtg agttcgatcc tgtgagcgtg
    18481 ccaagggttg ttagcgtcga agaacgaaac ctcggcgtgc cctcccaacc gagccttctc
    18541 tggcagcagg tcgaacgagg cgtagacctt gcgctcgtcg agggcgccgt cgaagtgaga
    18601 agcatggccc ccgagcatca aacggggttg ccaaggtgcg gccggggctt gaaacgccag
    18661 ctcaccgccg aagcgtgcca cgcgcgtcga gaccatgccg ttgaaggcgt gggggagcgt
    18721 gccgccgaac gcgctcacgc gcacgcccgg taatagtggc gcttccagcc tcacaccgtc
    18781 gagctggccc acgagcgcgg aggcggcgcg aaggcggccg agagaagccc ggaagtccgt
    18841 ggcggtgccg taagtcaggc tgagctcacg cacacgcaac acctggcggc tcgccgcgcc
    18901 aggccgcaat gagaagtcgt cgccggcgaa ggtctgcgca gcaacatctg cgtcgagccc
    18961 ccacggcgaa cgagtcatcg actcgtagtg cagacgcccg cggagctcgt tggccagcat
    19021 cttcgagcgc ccttggctgg gcagaagcgc gtagaatgcg tcgctgaaca ccagacgatt
    19081 gcgtgaccgc gtgcgcgtac cgagcggcac cggcgtcggc tgctgcgtca acgccggggg
    19141 aacgccgcgc agtcgattgg aacggaacgc cgagagtggc gtaggcttcg ccagggtgag
    19201 aacggtctcc tccgggcggt tcgtaacgat gttcacctgc ccgcgcgccc cgaccgccaa
    19261 gggtctgccc agcaaggcga ccgccgcgaa cgagctgctc acggagacca ccttcaacct
    19321 acgcccgccg aaggccagtt cgtcacccac ccgcagccca tccgcggccc cgggctccac
    19381 gtacgcacgc tcatcagtgg cgtccaccac gcgcacggcg atggctctcg tgacagtttg
    19441 cgcctgggcg gggctcacgg agcccacgcc gaacagtatc agcaagagcc ccgccccgcg
    19501 gatcatcggc gagccccgcc ggggtggcag tgcaggcaac cggagatgac gggcgccgtc
    19561 ccgcgatgct gtgcgatcag gactggcagc gagtgagcat ggcagctcgt gcagaccacg
    19621 agccgcggag cctctgattg ggcgtgacag tctccgcagg ctgcacgccg atgtttcccg
    19681 aaagatatag ggaacacggc atcgtgctgc agggccacta gaggctgtcc ggtgctgcga
    19741 aacgtgaatg ttgccggcgc aaaggccggg gaccaggaca gggtacggtg acagctgccg
    19801 cagttcttcg gcaaggctgg cttgctgacg tcaccggcca cgccgcgatg gagcggatgt
    19861 atatcgccgc gctggtaatc gccggcgtgg caggcgtagc aatccgccgg agcgccgcgc
    19921 cactgattct cgctcgcccg aacgtggcac tcgctgcatg ccgcgagcgt gtgcatgccg
    19981 ctcagcggca agcgggtatt gcggtggaga cgaatgcccg agaccgccgc ccagctagag
    20041 gcagtatggc agcggtcaca agcttgaccg agctgccgct gatgggcatc ggcgtggcac
    20101 cccacgcatt cacgggtgat cgcgcgatcc gctcgatggc agtccacgca attgccggcg
    20161 cgatgctgac ccgtcaacgg aaaacccgtt cgcgaatggt cgaacccgct ctgaccggcg
    20221 gtcttatgag cgatgctcca agagctctgg ctgtggcaac tcgagcagtc cagcccctcc
    20281 gtaatggaca catggcgccc cggctcggcg acggcgcgga accccgccgc ggagatcagc
    20341 ccgcaagcta gccaggctgc gattcgcgcg tgcttcaagg taggcctccc gagctcgatg
    20401 ccagcgtgcc ctttcgctcg gcgtggggat cggcgtggca atcccggcaa cgccggtaac
    20461 ccagccgaaa cagcgcagcc ttgccgccgc ctggcgtcgg cttgtgcgtg tggcacgcgt
    20521 cgcacttcaa ggctttgtgc ttcccaagga gctcgtaacc cgtgagcctc gtatgatcga
    20581 agtcggggac cttgaactcc accgtcgaat ggcagaactc acatgctcgc ttgggctccg
    20641 ccagacggaa ttgccctcga tgcacgtcgt cgtggcagcc gtcgcaggcg cgcggggcca
    20701 gccgatagct gggccccgcg cccgccttcc ggtcctgttc ggtgggcgta tggcacgcgg
    20761 cacacggcgt ggaagcgtgg gcgccggtga gcggccaaac actatgatcg atgcgcggca
    20821 gcgtccagga agcgggcgtg tgacaagtcg cacagccatt gcgccgcatc tcggcctgga
    20881 attggtcgcc gtgcgggttc tcgtgacact cggcgcaccg gagtttcttc gagctccagt
    20941 cgagccgctc cctgcgcttg gggtcgtgtt gatggcactt gccgcacgcc accgcggtgt
    21001 gacgcccctc gagcggaaag cgcgtgctgt ggtgcagggt cactccgaag cgagacgggc
    21061 gaaacccaga ctcactatga caggccttgc actcgccgtc gtctcgcttc gcgaatcgcg
    21121 cgtcatggac gtccttgtgg cagcggctgc agcgatcgaa ctccagctgt cgatagcgct
    21181 ctgcctccgg caaactgggc ttgtgacagc ccgcgcaggg cgactccagg tgcttgccgc
    21241 gcaaagggta gacggtcttg ccgtgcgcgt tcaatccgac actgcgagcc agaccgagcc
    21301 acaggatcac ctcgtgacag ctctcgcagc gttttccgaa gttcgcctcg tgtaccggtt
    21361 tgtggcagcc cgcgcaggcg cggccgcgtg agggtgtgcg aagagttccg cgatcgtggc
    21421 acttgatgca cggcgtgcgc gcgtgtccgt tggccaggct caaccaaggg tgattgcgga
    21481 cgccgacggt cacctggtgc caatcactct ccaaatgaca agactcacac gcgggcccga
    21541 gtttgccgtc gtgagggtca ttgtggcagc tggtgcagct cgaaaactcg agtcccttgt
    21601 acttgggagg gctaccgcga tgacacgccg agcactccac tttcac
//
LOCUS       DASTKU010000564        21789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_110153, whole genome shotgun sequence.
ACCESSION   DASTKU010000564 DASTKU010000000
VERSION     DASTKU010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 21789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21789
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110153"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VFQ61_19555"
     CDS             <1..1066
                     /locus_tag="VFQ61_19555"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9956706.1"
                     /translation="SVFLLLHDIRAYRSATIEFIGNTIVGPLLRPVIERTQRIGRAAQ
                     DVHLTGETGVGKEIFARLYHAASPLAAGPFLAVNCATIQKELAEATLFGSKRGAYTGA
                     HADQPGLFRSADGGTLFLDELGELDLSLQAKLLRVLELREVTPVGSTQPQAIRVQVCS
                     ATLRDLRERVRLGQFREDLYFRLARPSVAIPALRDRREEIAYLAHQAATGVSQNLRLT
                     PVLVEAAVSRPWPGNVRELLAAVRDGAHAALEEGSAHVKLSHLDPGAGLGFDLGTSNS
                     PKPPHALASAPVRSVDATTEPGQNPDLRAKVEDRLRRTAGNVSRTARLLGWHRTQLRR
                     YLEREGIDPQTFSGMTDETD"
     gene            1089..2066
                     /locus_tag="VFQ61_19560"
     CDS             1089..2066
                     /locus_tag="VFQ61_19560"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9956707.1"
                     /translation="MRSKPRHETPAVGPERRFARYPAPALRGHVERLWGWENAGPLPL
                     PLVTPGLGAEIFFHHGTALRSSVQGVEREHSQGHLLCVRHAPLQFLEQRDVGFTVARI
                     RAGALGRFTRIPLRDLKDSQLEVPEIWGRAGAETVERVADAKTQEERATIIEQFLLDR
                     LLETQVDAIAERALQLIYRCPDISIPELAEGCGLGRRQLERRVTNYFGQSPVELRCLA
                     RFYRVVRRLVLEPSRDTLQSGLEAGYFDQSHLIRDFKRFTGMTPQAFRNQAAAKTHFY
                     NPSSRAGSSLGFDSRSTRDDSLARRASRSATALGNRSHEVARAARIDRE"
     gene            1972..2283
                     /locus_tag="VFQ61_19565"
     CDS             1972..2283
                     /locus_tag="VFQ61_19565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HET9956708.1"
                     /translation="MIALHAELRVRPPHWETVLTRLRELLALTENEPGNLIYAIHSAE
                     GELVLYELYRDRAACDDHLASPVVVAALEGFAELLLEPPRVRVVELLNARMALPPGLH
                     P"
     gene            2310..2816
                     /locus_tag="VFQ61_19570"
     CDS             2310..2816
                     /locus_tag="VFQ61_19570"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9956709.1"
                     /translation="MAALFAGCASPGGTVHWPEPLSPSNADLFSHNDVVIARPCQRIW
                     DILVNAPKWPEWYANASQVRLLDRSRTQLEHGSAFTWRTFGIVVESRVAEFEPAARLA
                     WFGAAKDMTAYHAWLLKPLPDACRVITQETNNGPAAVALRDKNPDAIHDGHATWLAQL
                     KAISERSE"
     gene            2983..3579
                     /locus_tag="VFQ61_19575"
     CDS             2983..3579
                     /locus_tag="VFQ61_19575"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET9956710.1"
                     /translation="MMLGVLVVLALVSLGLIGFGVSSYNGLVSLRNRYRNAFEQIDVQ
                     LKRRYDLIPNLVAVAKGYLKHEHETLVAVTRARAAAVLASDQARAAPGSASAMGALGA
                     AEGALGSALGRLMVLTESYPELKAQTSMSDLSEELRSTENRVAFARQHYNDAVQSYDD
                     RRLSFPSNVVANLFGFEAAARLESISDPRERQAPKVEL"
     gene            3586..5586
                     /locus_tag="VFQ61_19580"
     CDS             3586..5586
                     /locus_tag="VFQ61_19580"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET9956711.1"
                     /translation="MDFFAKQEQARRATSWLLLLFVAALVCIFGAVYGAVSVGVATYE
                     SLAVVPGAFEWFSLERAVITLSIALVVVGVSALLKHAELRRGGAAVAVSLGATPVDVS
                     TRRRLERRLVNVVEEMAVAAGTPVPLVFVLEREQGINAFAAGYELSDAAITVTQGALE
                     NLNRDELQAIIAHEFSHILNGDMRLNTRLVSVLHGILTLTIAARLLRGAISRSSADGG
                     NSQGDSSPRSSRSRISFSSFRSNSGSSSSSRSDSSRGSSAGAAVALLIAIVVTIVLVT
                     LVGAVGSFFARWIQAIICRKRELLADAVAVQLTRNEAQVRRALRRVEGDSVGALLMSP
                     NASSVAHMFFAEGVRGWLPSHPPLGERIEQLGGVSPKSEPLPRVQNPSGQVFVPPQRL
                     LSVVGSMKLEDLEYAAGMLAALPDGLRDALADPVMAEALTYLMLLQPDPNARQEQWLS
                     VESTLDGEVIAAMHELGPALVGLEPRHRLPLLELALPSLRTMASQARARLLSNIDQLI
                     DSDGKTELFEFVLRCLVQARLTHASPLARSTAKRAACRQAARVVLTVMARLEIADPET
                     RARLVRESSFDAGYDPPLEIELEEGSDWVAFRSALDVLHRARPRAQRRFLDACERLML
                     ADGKLSLAECELFRAIVVSVGAALPARAVQRQPDVGEAARAL"
     gene            5558..5842
                     /locus_tag="VFQ61_19585"
     CDS             5558..5842
                     /locus_tag="VFQ61_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956712.1"
                     /translation="MSVRQLELSELVRGQLLAGRACSRGLTVEISAIGSARFCAVGGG
                     VYGVRPARWVRRALRPNGCAMVSLGRVVALVVPVPLAKALEHIGWQLGSR"
     gene            complement(6163..6381)
                     /locus_tag="VFQ61_19590"
     CDS             complement(6163..6381)
                     /locus_tag="VFQ61_19590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956713.1"
                     /translation="MISEISTPALGVCVGVSGIALGVSGIELGVSGVELGVSGVELGV
                     SGVELGVSGVELEEQAANSTASTGPTND"
     gene            6380..8548
                     /locus_tag="VFQ61_19595"
     CDS             6380..8548
                     /locus_tag="VFQ61_19595"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9956714.1"
                     /translation="MYHPFGETGAQDEHEFVELYNAGSGTADLGGYKLLVDGSERLTL
                     PLSTKLAAREYLVLAKNAAALLQEVRYALDAERVIGDYTGELDNGGAEVSLLNPLGTV
                     IDSVKYDDAAPWPLGADAFGAKPEFLPGLAPFDAHRFIGHSLERVDFTSPSSAPWKWE
                     ASGVDQATPGRPNGAVGAGLVVVSRQVTLEGTPDSAVISPDLPVQISFQLAGNLASGN
                     LTEAPLIEFRSDVLGMAAETSSMLPLQRIDERNYRVVLPGAAGDTVVRYRVRASSAAD
                     SRVLGPRRADPNAWYAYFVEPDPDSAASGRGHRMFIAPDNWTQLWQNISPGMNTGCTM
                     NPSWDAEVPAVFVSGGNVYDVLTRYQGSYNRRKDGIEIANFTAPAPTAPSPLLALSFH
                     IRFPNYAEYDGENSLNLNKLKQACPGVLNALEGALFEQAKIPAQSFKFARLYVNGGYY
                     NYGMEVRSIETSTMRAYEGKGNPVGLLFKADGASGRGPLGLANFTPLAGACSASALDR
                     YQRTYELKTHEELLQSGEAHAELISLIEELDRIQATSDSDPTVRAYFERNFDVSEVAT
                     QFAIRNWAGVWDDGFHNFWLYKRGSDGKWIILPQDFDCDFGGDPVQCADLGKFYNSPT
                     LSFFHPEQGDGVTAVAISRLKTQFIRAFRPEFSARVIELGTTLFSESNIDATLERILS
                     TFDMAAWDASPSRYCDVNARVQQARDFLAERRAFLAQGIQ"
     gene            8673..9377
                     /locus_tag="VFQ61_19600"
     CDS             8673..9377
                     /locus_tag="VFQ61_19600"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9956715.1"
                     /translation="MTSALIGLCVLVFALLAWVDQRLPIGFSGGPNSLRLSTLYRFGV
                     LSPIVVGLEPWRLLSAVFLHFSLAHLAMNMWGLWGLGTNLERRFGGARAAVLFFTTGI
                     GGFYVSTLYYGLESRVPTAGASGAVFGQLGGLIGILVARRMPGWRELLVRNVFMALLM
                     GFALRVNNAAHFGGLAIGWALGFLFEKEPVHKLTTRILSVVAALCAIASVASLVLSSA
                     SPIWQSVREEEAQIGR"
     gene            complement(9417..11003)
                     /locus_tag="VFQ61_19605"
     CDS             complement(9417..11003)
                     /locus_tag="VFQ61_19605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HET9956716.1"
                     /translation="MNAVHLFELMIAMLFAVIALHYIAHRLRFPPAGALIVGGAALAF
                     VPGLPHVSLDPALVLVIFLPPLLMDGAWFIPLRHLRRHLIGILSLAIGAVVFTTAAVA
                     VVTHALLPSLPWAACAALGAIVSPPDAVAARAVLQRVHLPQRLSILLEGESLLNDATG
                     LVLFRFAVAAAVTGTFSTMDALGSFALLALGGAAVGAAVGAAWVSVVRRLGDEYLVVA
                     SSVLLCWAAYILAEMLHVSGVISTVVAGLICGWYQHVVFSAAIRLRSTSFWTVMIFLL
                     EASVFMLIGSSLRDVIQRAGGFAVVVEHMTLPIVWILLAMTLSRFAWIFACDGVIALR
                     RRFGWRRFETMGWRASTVLSWAGMRGVVTLAVALSVPADLPGRDFILVAAFAVILVTV
                     LLQGTTLGLLIRRLRPPEAGAHVAPLTMSQAEAAMAQAQAKLIESRAYAPDGTLDHPQ
                     LLERYRQRATISKGYAGNEAQYQPRLQAHFDLVLEANAAGRAELLRLHRAGQIDDRVL
                     HELERDLDLEELAALSAKAT"
     gene            complement(11237..12151)
                     /locus_tag="VFQ61_19610"
     CDS             complement(11237..12151)
                     /locus_tag="VFQ61_19610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956717.1"
                     /translation="MPSAYGMVTLAVAQKMLANRVKGNLAFKPDHGEHGQCSWFVSSG
                     NPYTGITPGKDIRVNIEVEHANIVRDDSIVNDTLASFKDHERALRESNRRAFDLLLWN
                     TIGSHLRKTRPSRCSNVLVPQGEVSRQGGGYFLVVPPFAAGTVKVTNWGEIETIVLAR
                     SPNFLRNCLAAEETKNTNLSARVTVELGPCESNAAITLLRQRLQAAYQGATATLGAAA
                     PRVVAIPSGWNGATKNVTRFAATDQLVYRNFREARNAFKELMRLAAALNSGGAQASYV
                     KVFGGADWNVKQSSLYTAPNQPRQRFAP"
     gene            13379..13930
                     /locus_tag="VFQ61_19615"
     CDS             13379..13930
                     /locus_tag="VFQ61_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956718.1"
                     /translation="MVSREVGNILLDHVRNAGDPIGMVFEQVVTTNRLCSSMVVIPQL
                     FVDRVREYSRANRADGIILEAALKRAPVFREGGWVVALNSSAGAMTLDRHVFFGGALS
                     LTGYLHELVHVAQYAVAGRTRFLVSYFGLSAAEIARRLLLRRPLGAMKSSPHETQAYA
                     LEDRFADWLEATYPGQLMPMVRA"
     gene            13932..14330
                     /locus_tag="VFQ61_19620"
     CDS             13932..14330
                     /locus_tag="VFQ61_19620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956719.1"
                     /translation="MKLAYPRFNRVGLIACALELMLSCSYKTNLIPISTRKSDVRLKV
                     SPDHGRFLGPYLFTCLRVDRTNAPVGLIWSAHDRSGTGQHVKEVRYGVAPVGFTERAP
                     AKPIQVGDHVLVEIGCHGVTGRVEFVVSED"
     gene            complement(14609..15418)
                     /locus_tag="VFQ61_19625"
     CDS             complement(14609..15418)
                     /locus_tag="VFQ61_19625"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET9956720.1"
                     /translation="MHGSLGEKIGAGVFAEVHAWAPGQVVKLSKPGIPNWVLQHEARM
                     TRAVFAAGGPAPEVLDEVTLDGRFGIVLPRFDGPTLKQATLTGAITGEQAGAILAKLC
                     LAVHKTPPPADALSLRQAMNARLQFAGRVLPERIITGVLALIEHLPHEDVLCHSDLHP
                     GNVIMTAQGPRLIDWLGAARAPAAYEIAQCHVLISELDPEHADNAERATVNLALQAEY
                     ARLTGIEPAALTAAIQPYLPIVRVFLLLGGAMPEMRERLLQRVEMALRSED"
     gene            17380..18120
                     /locus_tag="VFQ61_19630"
     CDS             17380..18120
                     /locus_tag="VFQ61_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956721.1"
                     /translation="MGQATNDFKNRKRLFISGESRAVRRCADVIDAWIWWSYVGRAIL
                     SEILDAPAGRTVDFSVVPDNFIAGLDARASARSPNDAYGAGYSSPDLGITGTGMGSKV
                     SVVLTNSLSVSDGTCQAIMNVPCSSLAAFKTHFVLMHEIVHANRIARGKLRTVSMGGR
                     LKNSEEAIAIIVTNMLMSEKGESGLRKTYDTSDVLTITADEFVKQDGVESLVRTIAND
                     HPRLKTTLNRKEIPVPFNPIYSVFQGAP"
     gene            complement(18306..18581)
                     /locus_tag="VFQ61_19635"
     CDS             complement(18306..18581)
                     /locus_tag="VFQ61_19635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956722.1"
                     /translation="MTPTRTAEASPWLLEATHAGACPMVKLGQLPAEAFRYYDCRAGD
                     VLLMKPLLVHRSPRAANPARRRVLHVLYAPVEGWHGRLSRHTAQLTS"
     gene            complement(19179..19250)
                     /locus_tag="VFQ61_19640"
     tRNA            complement(19179..19250)
                     /locus_tag="VFQ61_19640"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19216..19218),aa:Thr,seq:cgt)
     gene            complement(19645..21093)
                     /locus_tag="VFQ61_19645"
     CDS             complement(19645..21093)
                     /locus_tag="VFQ61_19645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956723.1"
                     /translation="MTRTAALKRLLTLGLGWLCTACSMQPAAAPASATQSWRKIEFDG
                     ENVVVYTDMQGQGALDWARSLAQTRSAMLDLIWGNSKAPPAPTVVLMFRRPTDAGRFL
                     PPGALAMVRRSIPFGDRLVFSPGSKGIEGFISHEMAHHLSHFYMPNQPLWFAEGLATF
                     LETVRYDPQTGQATLGLMPPNVKEARTLATDLSIQGILDQKAYDHSVRGYGFYIGAYG
                     IFTNLVNRHEDDFAQFQRGLSRLQPWRAAWAQHLSDVTPERLGKELATKGVFTLVQRE
                     VSRINPDVHITPITSAQAHGLFATAAADSQLALEEARAGLIEDPNEILSLAALFHYGG
                     LPKTERQALVDKALKAHPESSRSWDMACMLSTDDAVRLKMADKAVALDPFNPSARIQR
                     AAARILNGRAESALADADLSIRLSYPSVFSLRVYAASLAANKRCDQALEALANELPGA
                     PAEELKNLRLGDTPAEQECARLAAQGSRAKAN"
     gene            complement(21296..>21789)
                     /locus_tag="VFQ61_19650"
     CDS             complement(21296..>21789)
                     /locus_tag="VFQ61_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956724.1"
                     /translation="MLDLIWSKSKAPPAPTVVLMFRRPMDARHFLPPGARAMVSRGLP
                     FGDRIVLSPEGKASQEVISHEMAHHLSHFYMPNQPQWFSEGLATFLQTVRYDPQTGQA
                     TLGLMPWNVKEAITVAADQSIQGILDQQEVDPSTPGCGFYVGAYGIFTNLVNRHGQDL
                     GYI"
BASE COUNT         4007 a         6488 c         7081 g         4213 t
ORIGIN      
        1 cagcgtgttc ttacttttgc acgatatccg cgcgtatcgg tcagcgacta tcgaatttat
       61 cggcaacact atcgtgggcc cattgctgcg cccagtcatc gagcgaacac aacgtatcgg
      121 gcgcgctgca caagacgttc atctcaccgg cgaaaccggg gtgggtaaag agatcttcgc
      181 gcggctttat catgcggcga gtcccctggc tgcagggccc tttctggccg tgaactgtgc
      241 gaccattcag aaggagctcg cagaagcgac gctcttcggc agcaagcgcg gggcttatac
      301 cggcgcgcat gcggatcagc cggggctgtt ccgcagcgca gacggaggaa cattgttttt
      361 ggatgagctg ggcgagctgg acttgtcctt acaagccaag ttgttgcgag tactcgagct
      421 gcgcgaggtg acacccgttg gaagcaccca gccgcaggcg attcgggtgc aagtttgctc
      481 ggcgaccttg cgtgatttga gagaacgcgt gcgattgggt cagtttcgcg aagatctcta
      541 ctttcggctg gcgcggccat ccgtagcgat tccggccttg cgggaccggc gagaggaaat
      601 tgcctatctg gcccatcagg ccgccactgg ggtatctcag aatctgaggc tgactcccgt
      661 tttggtcgag gcggcagtct cccgcccttg gcccggtaac gtgcgcgagc tactggctgc
      721 cgtgcgggac ggcgcccacg ccgcattgga agagggcagc gcgcacgtca aactttccca
      781 cctcgatccg ggggcaggac tggggttcga tttgggcacc tccaactcgc cgaagccgcc
      841 gcacgctctt gcgtccgctc cggttcggag tgtcgatgcg acgaccgagc ccggacaaaa
      901 tccagatctt cgtgcgaagg tcgaggatcg cttgcgtcgc acggcgggca atgtatcgcg
      961 aaccgcgcgt ctactgggtt ggcatcgcac tcaactccgg cgatacttgg aacgggaggg
     1021 catcgatcct caaacgttct ccggaatgac ggacgagacc gattgagtcg cggtcaacga
     1081 ggtcactctt gagatccaaa ccacggcatg aaactccggc ggtgggcccg gagcgtcgct
     1141 tcgcccgata tccggcgccc gcgctgcgcg ggcacgtcga gcggctgtgg ggttgggaaa
     1201 acgcaggtcc gttgccgctg cctctggtga cacccgggct gggtgcggag atttttttcc
     1261 atcatggaac agcgctgcgt tcgtccgttc agggggtaga gcgcgagcat agccaaggcc
     1321 acctgttgtg cgtgcgtcac gctccgctgc agttcttgga acaacgcgac gtggggttca
     1381 cggtcgctag gatccgggcg ggggcgctgg ggcgctttac tcgtattcca ctacgggacc
     1441 tcaaggattc acagctcgag gtccccgaaa tttggggccg ggccggtgcg gaaaccgtgg
     1501 aacgtgtggc tgatgcgaag acccaggaag agcgcgcgac tatcatcgag caattcctgc
     1561 tggaccgatt gttggaaacg caagtggacg cgatcgcgga gcgcgcgctt cagctcattt
     1621 accgatgccc ggacatcagc atccccgagc tcgccgaagg ttgtggcctg ggtcgacgtc
     1681 agctcgagcg gcgtgtaacg aactacttcg gacaatcacc cgtggagttg cggtgcttgg
     1741 cgcgattcta tcgtgtggtg cgaaggctgg tgctcgagcc ctctcgggac acgctgcaga
     1801 gcgggctcga ggcgggatat ttcgatcaat cgcacttgat tcgggacttc aagcgcttca
     1861 cgggcatgac tccgcaagca ttccgaaacc aagcggcagc gaagacgcat ttctacaatc
     1921 ctagctcgcg ggcgggttct agcttggggt tcgattcaag gagcacccga gatgatagcc
     1981 ttgcacgcag agcttcgcgt tcggccaccg cattgggaaa ccgttctcac gaggttgcga
     2041 gagctgctcg cattgaccga gaatgagccg ggcaatctga tctatgcgat tcacagcgct
     2101 gaaggggagt tggttttata cgagctctat cgagacagag ccgcctgtga tgatcatctg
     2161 gcctcacctg ttgtcgtggc cgcattggaa ggcttcgcgg aactgctact ggagcctccg
     2221 cgggtgcgtg tcgtggagtt gttgaatgca cgcatggcat tgcccccggg actgcaccca
     2281 tgaccggagc taaatcgagg gcagtactga tggcggcact tttcgccggg tgtgcttcgc
     2341 ccggcggtac tgtccactgg cccgagccac tgtcccccag caacgctgac ctctttagcc
     2401 acaacgatgt ggtgattgcg cgcccctgcc agcggatctg ggacatcttg gtgaacgcac
     2461 ccaagtggcc cgagtggtac gccaatgcga gccaggtaag gttgctggac cgtagccgca
     2521 cgcagctcga acatggcagt gcgttcacct ggaggacgtt cggtatcgtg gtcgagagcc
     2581 gcgttgcgga gttcgagcca gcggcgcgcc tggcctggtt tggtgcggcc aaagacatga
     2641 cggcgtatca tgcttggttg ctgaaacctt tacccgacgc ctgcagggtg atcacccagg
     2701 aaaccaacaa tgggcctgcg gcggtggcct tgcgtgacaa gaatccagac gcgatccacg
     2761 acggacatgc gacctggctc gcgcaactga aagcgatctc cgagcgttca gagtgagcga
     2821 cagacctgcg acgcgaccgg ttcttccgcc tgtattcccc gtcgcccgcc tggaccgctg
     2881 ttagcttcgc ttgccgcaat gtgcgctgcg gtagagttcg tcgttgctcg ggcgctggcg
     2941 tcgaccttgt cctggggccc ttgtctttgc tagctttctg ccatgatgtt gggtgttttg
     3001 gtggtgctgg cgcttgtttc attgggtttg attgggtttg gggtgagcag ctacaacggg
     3061 ttggtatcgc tgcgaaatcg ctatcgcaac gcgttcgaac agatcgatgt gcagttgaag
     3121 cggcgttacg atctgattcc gaacctggtg gcagtggcga aggggtatct caagcacgag
     3181 cacgagacac tcgttgcggt gacgcgcgcc cgcgcggcgg cggtgctggc ttcggatcag
     3241 gcacgcgcag ctcctgggag cgcaagcgcg atgggcgcgc tcggggcggc cgaaggggca
     3301 ctcggctcgg ctctcggcag gctgatggtg ctcacggaga gttatcccga gctcaaggct
     3361 caaacctcga tgtcggacct gtccgaggag ctgcgctcga ccgagaaccg agtagcgttc
     3421 gcgcgccagc attacaacga tgcggtccag tcctacgacg atcggcgcct gagctttccg
     3481 agcaacgtcg tcgcaaacct cttcggcttc gaagcggccg cgcggctgga gtcgatctcc
     3541 gatcctcgcg aacgacaagc cccgaaggtc gagctctagg ccgacatgga cttcttcgcc
     3601 aagcaagagc aagcgcgccg agccacgagt tggttgctcc tactcttcgt cgccgccctg
     3661 gtctgcatct tcggcgctgt ctacggggcg gtgagtgttg gcgtcgcgac ttacgagagc
     3721 ctggccgtcg ttcccggggc gttcgagtgg ttctccctgg agcgagctgt cattacactg
     3781 tcgattgccc tggtcgtggt cggcgtctca gcgctcctca aacacgccga gctgcgtcgg
     3841 ggcggcgcgg ccgtagcggt ctcgctcggc gccacaccag tggatgtttc gacgcggcgc
     3901 cgcttggagc ggcggctcgt caacgtcgtc gaggaaatgg ccgtggcggc aggcactccc
     3961 gtgccgctcg tcttcgtgtt ggagcgcgag caggggatca acgcctttgc ggctggatac
     4021 gagctgtcgg atgcggcgat cacggttact cagggagccc tcgagaacct gaatcgcgat
     4081 gagctgcaag ccatcatcgc tcatgagttc agccatattt tgaacggaga catgcgcttg
     4141 aatacgcgct tggtgtccgt gctccacggc attttgacgc tgaccatcgc cgcgcgtctc
     4201 ctgcgcggag cgatctctag gtcgagcgcc gacggcggga actcgcaagg ggacagcagc
     4261 cccaggagtt ctagatcccg aatctcattc tcgagcttcc gttctaactc aggatcgagc
     4321 tccagctccc gttcggactc gagccggggc agcagcgccg gcgccgccgt cgccctgctg
     4381 attgccatcg tggtgacaat cgtgctcgtc acgttggttg gcgcggtcgg atccttcttc
     4441 gcgcgctgga ttcaggccat catctgccgc aaacgcgagc tcttggccga tgcggtggcg
     4501 gtgcagttga cacgcaacga ggcgcaggtg cgtagggcgt tgcgccgggt ggaaggagat
     4561 tcggtcgggg cactgctcat gtcgcccaac gcgagcagcg tggcgcacat gttctttgcg
     4621 gagggcgtgc ggggatggtt gccttcacac ccgccgctcg gcgagcggat cgagcaactt
     4681 ggaggtgtct cgccgaagtc cgagcctctg ccacgtgtcc agaacccaag cgggcaagtc
     4741 ttcgttccac cccagcgcct gttgagcgtg gtgggcagta tgaaactcga agatctcgag
     4801 tatgcggccg gcatgctagc ggcgctgccc gacggtttgc gagacgcgct cgccgatccg
     4861 gtgatggccg aagcactgac gtatctgatg ttactccagc cggacccgaa cgcgcggcag
     4921 gagcaatggc tgagcgtgga gtcgaccttg gatggggagg tcattgcggc gatgcacgag
     4981 ctcggcccgg ccctggttgg gctcgaaccg aggcaccgtc tcccgctgct cgaactcgcg
     5041 ttgccgtccc tgagaaccat ggcgagccaa gcccgagcgc gtctgctctc gaacatcgac
     5101 cagctgatcg acagcgacgg caagaccgaa ttgttcgagt tcgtcctgcg ttgcctagtc
     5161 caggcgagac tgacgcacgc atcaccgctc gctcgatcca ccgcaaaacg cgcggcttgc
     5221 cgccaggccg cgcgggtggt gcttacggtg atggcgcggc tggagattgc ggaccccgag
     5281 actcgcgcgc ggctcgtgcg ggagagcagc ttcgatgcgg ggtacgatcc tccgctcgag
     5341 atcgaactcg aggaaggctc ggactgggtc gcgttccggt cggcgctcga tgtgctccac
     5401 cgcgcgcgtc ccagagcgca gcgtcggttc ttggatgcgt gtgagcgctt gatgcttgcc
     5461 gatggcaagc tctcgctcgc ggagtgtgag ctattccgcg cgatcgtcgt gagcgtgggt
     5521 gcggcattgc cggcgcgggc ggtccagcgc caacccgatg tcggtgaggc agctcgagct
     5581 ctctgaactc gtgcgcgggc agctgctcgc gggacgcgcc tgctcccggg gactcacggt
     5641 cgagatatcg gctatcggct cggccaggtt ttgcgcagtc gggggcggcg tttacggcgt
     5701 gcggcctgcg cggtgggtgc gacgagctct gcgcccgaac ggctgcgcca tggtgtcact
     5761 cggacgggtg gtcgcgctcg ttgttccggt gccgctcgca aaggccctcg aacacatcgg
     5821 ctggcagttg ggctcgcgct agtggagcga gcacgaggtg tgcgactggc cctaggctca
     5881 gacggaagca gggacgtgtc acttcggcgg gctggaacgg ctggggcctg cgggccaagg
     5941 tgagcccctg acggggcgcc gaggatgtac cctgagccgc gctgggcgtt gcgctttgga
     6001 aatcaatcgc gcatcgactt gcagacatgc agggggccca tcgctcgggg ttagaaaccg
     6061 ccgctcacca acgccaccgt gcgctgactt tttcttagct gtgagacgcg cttggcagcg
     6121 gacgtggcac actccgctcg ctttgaccgt tctatttcgc gttcagtcgt tggttgggcc
     6181 cgtgctcgcg gtactgttgg ctgcctgctc ttcgagctcg acgccggaaa caccgagctc
     6241 cactccggag acaccgagct cgacgccgga aaccccgagc tcgacgccgg aaacaccgag
     6301 ttcgatgccc gagacaccga gcgcgatgcc ggagacaccg acacagacgc cgagcgccgg
     6361 ggtgctgatt tccgagatca tgtatcaccc gtttggcgag accggcgcgc aggacgagca
     6421 cgagtttgtc gagctctaca acgccggcag cggcacggcc gacctgggtg gctacaagtt
     6481 gctggtggac ggaagcgagc ggttgacgtt gccgctttcg accaagcttg cagcgcgtga
     6541 gtacttggtg ttggcgaaga acgcagcggc attgctgcaa gaagtccgct acgcgctcga
     6601 cgccgagcgt gtgatcggcg actacacggg cgagctcgac aatggcggcg ccgaagtaag
     6661 cctgctgaac cctctcggca cggtcataga ttccgtgaag tacgatgacg ctgcgccctg
     6721 gccccttggc gccgatgcat tcggtgccaa gccggagttc ttgccgggtt tggcaccgtt
     6781 cgacgcgcat cgtttcatcg gtcactcgct cgagcgcgtc gactttacga gcccgagctc
     6841 ggcgccctgg aagtgggagg cttccggcgt ggatcaagcg accccgggcc gaccaaatgg
     6901 ggcggttggg gccgggctag tcgtggtgag tcggcaggtc acgctcgagg ggacgccaga
     6961 ctcggcggtg atttcacccg acctcccggt acagatctcg tttcagctag cgggcaacct
     7021 tgcgtccggc aacctcactg aggcaccgtt gatcgagttt cggagcgacg tactcgggat
     7081 ggcggccgag accagcagca tgctcccgct ccaacggatc gacgaaagga actaccgcgt
     7141 tgtgctgcct ggggcggcag gcgacacggt cgtccgctac cgggtgcgtg ccagctctgc
     7201 cgccgactcc agagttctag gcccacggcg tgctgatccc aacgcttggt acgcatattt
     7261 cgttgaaccg gatccggatt ccgctgcatc ggggcgcggc catcggatgt tcatcgcgcc
     7321 cgacaattgg acccagcttt ggcagaacat cagcccgggc atgaacaccg gttgcacgat
     7381 gaacccaagt tgggatgccg aggtccctgc ggtcttcgtg agcggcggca acgtgtacga
     7441 tgttcttacg cgctaccagg ggagctacaa ccgtcgtaag gacgggatcg aaattgcgaa
     7501 tttcacggct cccgcgccga ccgcaccgag tccgttgctc gccttatcgt ttcatatccg
     7561 cttcccgaat tatgcggagt acgacggcga gaattcattg aacttgaata agctcaagca
     7621 ggcttgcccc ggcgttctga atgccctgga gggcgcgcta ttcgagcagg caaaaatacc
     7681 agcccagagc ttcaagttcg cccgcctcta cgtgaatggc gggtattaca actatggcat
     7741 ggaggtgcgc agcatcgaga cgagcaccat gcgcgcttac gagggtaagg gcaatccagt
     7801 gggtctactg ttcaaggctg atggcgcctc cggccggggc cccttgggtc tcgccaactt
     7861 tacaccactg gcgggggcgt gcagcgcttc ggcgctcgac cgttaccagc gcacctacga
     7921 gctcaagacc cacgaggagc ttctgcagag tggcgaggct cacgcggagc tcatatcctt
     7981 gatcgaggag ctcgatcgga tacaagccac gtcggatagt gacccgacgg tgcgggctta
     8041 cttcgagcgt aacttcgacg tatccgaagt cgcgacccaa ttcgcgatcc gaaactgggc
     8101 cggggtttgg gacgacggct tccacaattt ttggctctac aaacggggta gcgacggcaa
     8161 atggatcata ttgccgcaag acttcgactg cgacttcggc ggtgacccgg ttcagtgcgc
     8221 ggatcttggc aagttctaca attcgccaac cctgtcgttc ttccacccgg agcagggaga
     8281 cggagtgacc gcagtcgcga tttcgcgcct caagactcaa ttcatccgcg ccttccgacc
     8341 tgagttttca gcgcgcgtaa tcgagctcgg gacgacgttg ttcagtgaga gcaatatcga
     8401 cgcgacgctc gagcgcattc tatcgacctt cgacatggcc gcttgggatg cttcaccgtc
     8461 gcgctactgc gacgtcaacg ctcgggtgca gcaagcgcgc gatttcctgg cggagcggcg
     8521 cgccttcctc gcgcaaggca tccaatgatc gaaattcgtc caagcggcgc gagcgccgcg
     8581 gtgcgtgcgt tgcggtgagc ctgcgttgcg gtgagccctt gcctcaactt caagcgtcga
     8641 acatatggtg gaaaaatctc cgcgagtttc cagtgaccag cgcgttgatt ggtctttgcg
     8701 tcctggtgtt cgcgctgctc gcgtgggttg accagcggct tccgattggc ttcagcggcg
     8761 gaccaaactc gcttcgactc tcgacgctgt accgcttcgg agttctgtcg ccgatcgtgg
     8821 ttgggctgga accctggcgc ttgctgtcgg cggtcttcct gcacttcagc ctagcccacc
     8881 tagcgatgaa catgtggggg ctctggggcc tcggcacgaa tctcgagcga cgcttcgggg
     8941 gcgcgcgcgc ggcggtcttg ttcttcacta cggggatcgg cggattctat gtcagcacgc
     9001 tttattacgg gctcgagagt cgcgttccga ccgcaggcgc gagtggcgcc gtcttcggcc
     9061 agcttggtgg attaatcgga atcctggtgg cgcgccgaat gccggggtgg cgggagctgt
     9121 tggttcgcaa cgtctttatg gccttgctca tgggtttcgc tctccgcgtg aacaacgccg
     9181 ctcattttgg tgggctcgcg atcggttggg ccctcgggtt tctcttcgaa aaggagcccg
     9241 tgcacaagct gacgacgcgc attctgtccg tggtcgcggc cctgtgcgcg atcgcgagcg
     9301 tggcctcgct ggtgctttcg tcggcttccc cgatctggca aagcgtacgc gaagaagaag
     9361 cccagatcgg acgctgatga ggacgctctc gccgattatt ttctgacccg agcgtctcag
     9421 gtcgctttcg cggacagggc cgctaactcc tccaagtcca ggtcccgttc caattcgtgc
     9481 agcacgcgat cgtcgatttg tcccgcgcga tggagccgca aaagttccgc gcgccccgcc
     9541 gcgttggcct ccagcactag atcgaagtgc gcctgcagcc gaggctggta ctgcgcctcg
     9601 ttacccgcgt agcctttcga gatggtggcg cgctggcgat acctctcgag cagttgtggg
     9661 tggtcgagcg tgccatctgg cgcgtaggca cgcgactcga tgagtttcgc ttgggcctgc
     9721 gccatcgcgg cctccgcttg cgacatagtc agtggcgcga cgtgcgcgcc ggcctcgggt
     9781 gggcgtagcc ggcggatgag caaaccgagc gtcgtgccct ggagcagcac cgtcaccagg
     9841 atcactgcaa aggccgcgac cagaatgaag tcgcgaccgg gcagatccgc gggcacactc
     9901 agggccacgg ccagcgtgac aacaccacgc atgccagccc agctcaggac cgtcgacgcc
     9961 cgccagccca tcgtctcgaa gcgacgccag ccgaacctgc ggcgcagcgc gatgacgcca
    10021 tcacaggcaa aaatccacgc gaagcgcgac agtgtcatgg ccagcaggat ccaaacgatc
    10081 ggtaacgtca tgtgctcgac caccaccgcg aaaccacctg cgcgttgaat gacgtcgcgc
    10141 agcgacgagc cgatgagcat gaacacgctg gcttcgagca agaagatcat cacggtccag
    10201 aacgaagtgc tgcgcagccg aatagcggcc gagaacacca cgtgctggta ccaaccgcag
    10261 atgagaccgg ccaccaccgt ggagatgacg cccgatacat gcagcatctc cgccaagatg
    10321 tacgcggccc agcacagcaa caccgacgag gcgacgacca ggtattcatc gcccagtcgc
    10381 ctcaccacgg agacccaagc tgcaccaacg gccgcaccaa cggccgcgcc gcctaacgcg
    10441 agcagggcga agctgccgag cgcgtccatt gtgctgaacg tgcccgtcac cgcagcggcg
    10501 accgcgaagc ggaacagcac caagccagtg gcgtcattca gcaggctctc gccctccagc
    10561 aaaatggaca gccgctgcgg caggtgcacc cgctgcagca ccgcgcgcgc ggcgacggcg
    10621 tctggcggag agacgatcgc gcctagtgcc gcgcaggccg cccagggcag tgagggcaac
    10681 agcgcgtgcg tgaccactgc taccgctgcg gtggtgaaca cgaccgcgcc gatggccaac
    10741 gacaggatgc cgatgagatg ccgtcgcaga tgccgcaggg gaatgaacca cgcgccgtcc
    10801 atcagcagcg gaggcaggaa gatcaccagc accagcgccg gatccaatga cacgtggggc
    10861 agaccgggaa cgaacgccag cgccgcgcca ccaacgatca gcgccccagc tgggggaaag
    10921 cgcagccggt gcgcgatgta gtgcagcgcg atgaccgcaa acaacatcgc gatcatgagc
    10981 tcgaataggt ggacagcgtt cattccagat ctccagaagg cggctgtagc ctaactggga
    11041 cgcgcgatca tcaggttgtc gagtccgggc gcgcgatgtt gagctttgcg ggcatgccgc
    11101 tcacgctgac gagcaaggcg gagtggtgcg agccattccg gttcgaactc tcagcagacc
    11161 tcgagctcgg gcgtcacctg acgtgacagg ggagctcggg gcgagcccaa ggacccccaa
    11221 cgttgcgaga gcggcctcaa ggtgcgaagc gctgacgggg ctggttcggg gcggtgtaaa
    11281 gtgaactctg cttgacgttc cagtccgccc cgccaaagac cttgacgtag gaggcttgtg
    11341 ctccgccgct attgagggcc gcggccaagc gcatgagctc cttgaaagcg ttccgtgcct
    11401 ctcggaaatt ccggtagacg agttggtccg tagcggcgaa gcgcgtcacg ttcttggtcg
    11461 cgccgttcca tccggatgga atggccacca ccctgggcgc agccgcaccc agggtcgccg
    11521 tagctccttg ataggctgcc tggagccgct gccgaagcag ggtaatggct gcatttgact
    11581 cacaagggcc caattcgact gtaacgcgcg ccgataggtt cgtgttcttg gtttcttcgg
    11641 cggcaaggca gttccttagg aaattagggc ttctggctaa gacgatggtc tcgatctcgc
    11701 cccaattcgt cactttgacg gttccggcag cgaacggagg caccaccaag aaatatcccc
    11761 caccttggcg ggatacctcc ccttggggaa ccagaacgtt ggagcaacgc gaaggccgag
    11821 tcttgcgaag gtgagagcca atggtattcc agaggagcag atcgaaggcg cgtcgattgc
    11881 tctccctcag ggcccgctcg tgatctttga aggacgcgag ggtatcgttt acgatgctat
    11941 cgtccctaac gatgttcgcg tgctccacct cgatgttgac gcggatatct ttgccgggag
    12001 taattccggt ataggggttt ccagagctca cgaaccagga gcactggcca tgttcgccgt
    12061 gatcgggctt gaaggcgagg ttccctttga ctcgattggc cagcatcttc tgcgcgaccg
    12121 ctaaagtgac cattccatag gcactcggca ttggctttga cccctagtag ttatttcgga
    12181 ggctgcgtac acgtctccgc taagccgggg ctcccggagc actgactgac ggatgcacgc
    12241 gagttctttg ttgggttgtg ttgactcaga gcttctacga aggtctgatg ttgactttta
    12301 actgttaaag agaggtgcgc cgagtgtcaa cgccaaagcc gcgcgtccat cagatactca
    12361 tggcctaaag actaaacggc atctcaatca tcgaggacta ctccctaaac aagcgatagg
    12421 cgaagcgttg agttctacat cgcgacggat ccgcgcgacc gctcggaggc agaggcccag
    12481 tccgaggaat gccgccgcca tctcgcccag gtgcgagtgg agacctctga acctggcgcc
    12541 gatgtgcacg ttgacggtaa accagttggg accggaccgt tcctctccga ggtctggctc
    12601 gagccgggcg agcatagctt cacggcaaca tatcgacggc ccgcagcagg ttggtgcgtt
    12661 gaatctttcg cgagtcgtca gaaacgttcg ggcgtcacgc tgacaccgtt cccggggttt
    12721 ctggcccggt caggcgtgca tcgaccgcga cgtcgactcc cgcgccgacc gagtgcggcc
    12781 ggcaatggga gcacgtgtcg ccccgccgct tcggaagttg tcgggggcta ctcatcatca
    12841 gcgacctcga gataggtgct agaagtcgca cggccgccct tgcgtaggcc caagggacgc
    12901 tgtcagaccg tgccctggct cttggccaaa tcgtgcacaa agtcaagctc gcgtgggacg
    12961 aagaagtgac tgaagcctct gcgagctcgg cggcctcgat gtacgcgcgg agccttgctc
    13021 cccctgtacc cttcgagttt cgcgctgacc atccattcct gttcctcttg ggagacaagg
    13081 catctggagc ggtattgttc atcggccggg gtcagccaaa cgcacgaatt gggagggagc
    13141 cgtcgataag tgcgtatgac cgactctccg cgctcatgtt gcaaccttac cttcgaagct
    13201 ctcgtccctc gaatcggccc taccggaggc ggagaggcga ttggattcag cagcgggcca
    13261 tcggcgttgc tcccgcgccc gatagccgtt tggaaaccag cgttcacggc ggctccacgg
    13321 cctctcaaga gcgtgcaggc atgttgacat caaccgcgtt gccctctacg gtcaacccat
    13381 ggtgagcagg gaagtgggga acatcctgtt ggatcacgtc cgcaacgcgg gggatccgat
    13441 aggcatggtg ttcgagcaag ttgttacaac caaccggctg tgttcgtcga tggtcgtgat
    13501 cccgcaactg ttcgtcgacc gcgtgcggga gtattctagg gcgaatcgcg cagacggcat
    13561 catcctcgaa gcggccctca agagagcccc ggtcttccga gaaggaggct gggtagttgc
    13621 gctaaattcg agcgcgggcg cgatgacgtt ggaccgtcat gtcttctttg gtggtgccct
    13681 ctcgttgacc ggctaccttc atgagctcgt gcatgtagcc cagtatgccg tagccggcag
    13741 aacacggttc ctcgtgagct acttcggact ttcggctgcc gagattgctc gtcggctact
    13801 cttgcgtagg ccgctggggg ctatgaaatc tagtcctcac gaaacgcagg cctatgcatt
    13861 ggaggatcgc ttcgctgatt ggcttgaggc cacatacccc ggccagctca tgcccatggt
    13921 cagggcctga tatgaagctc gcgtatcccc gattcaatcg tgtggggctc attgcctgcg
    13981 cgttggagct aatgctctca tgctcctaca aaacgaacct cattcccatc tccacgcgca
    14041 agagcgacgt gcgcctcaaa gtgagcccag atcatggtag atttctaggg ccttacttgt
    14101 ttacgtgtct cagggttgac cgaacgaacg ccccagttgg cctgatctgg tccgcgcatg
    14161 accgaagtgg cacgggccag cacgtcaaag aagtgcggta cggcgtggcg cccgtaggtt
    14221 tcaccgaacg tgcgccagcc aagcccatcc aggttggcga tcacgtattg gtcgagatcg
    14281 gttgtcacgg cgtcacgggg cgggtggaat ttgttgtttc ggaagattga gtgcgtgctc
    14341 ctgtgacggt gagcgcggcg ttgttcggat gcgaacgacc gctgactcac ggcgctccac
    14401 cgtgagtgcg gtaaccgcgc gccccagcaa cgcctcgcgc gcacgccgct tggtttcttg
    14461 agagtaggag ggaagggaca tactgacgcc accgtccact attccgtccc agaagaagct
    14521 ggcccgaggt ctcgatgtca gaactcggcc tatcggatcg cggttggatc cgccaagcgg
    14581 atcaggctca gtccgggcaa gcggctgctc aatcctccga gcgcagggcc atctcgacgc
    14641 gctggagcag ccgctcccgc atctcaggca tggccccgcc gagcaggagg aagacgcgga
    14701 cgattggcaa atagggttgt atcgctgctg tcagcgccgc cggctctatg ccggtcaacc
    14761 gcgcatactc cgcctgcaga gccaaattga ccgtggctcg ctccgcattg tcggcatgct
    14821 ccgggtccag ctcggaaatc agcacatggc actgtgcaat ctcgtaggcg gcgggagcgc
    14881 gagccgcgcc gagccagtcg atcagtctcg gcccctgcgc cgtcatgatc acattgccgg
    14941 ggtgaagatc ggaatggcac agcacgtcct catgcgggag gtgttcgatc agcgccagga
    15001 cgccggtgat gatgcgctcc ggaagcacgc ggccggcaaa ctgcaatctg gcgttcatcg
    15061 cctggcggag cgagagtgcg tccgccggcg gtggcgtctt gtgaacggca aggcagagct
    15121 tcgctaggat tgcgcccgct tgttcacccg tgatggcgcc ggtaagcgta gcctgcttga
    15181 gggtcggtcc atcgaaccgc ggcaacacga tgccaaaccg cccatcgaga gtgacttcgt
    15241 cgagcacctc cggcgccggg ccgccggcgg cgaagaccgc gcgggtcatt cgcgcctcgt
    15301 gctggaggac ccaattgggg atgcctggtt tggacagctt gacgacctga ccgggcgccc
    15361 aggcgtggac ctccgcgaag acgccagccc cgattttctc tcccaacgat ccgtgcatgt
    15421 ccgttctccg gcctgcaaaa ggaaacaccg gcggggcgca aaaagcaact catcccgtct
    15481 cgggccgccg ggacactccg atccgggcag cattgtggag gctcccggag caaacgacac
    15541 gttccattcg agcggtctcg agctttctcg cttggcagaa ctctctagcc ccgcattccg
    15601 tgcgaccggg ccgcgacccc cgagtaggtt catgagctcc aacacgaccg ctgtcgtatc
    15661 ggagccgtcg tcagcgggag acgcgtgcga ctcgagaccc aacgcgagat cgattggacg
    15721 acccgcttct tagaagtcgt ccagcgccga aagggacggc tgtcaccaac gacgagcatc
    15781 ggccgcatgc acaccgagaa tatggcaagt ggccacgagc acgcctggtc gcttaaggcg
    15841 agcacgctcg gtccaaacat cgcgctcgtc gtcgagcgta tgctcggccg acactcgcga
    15901 cccgagcttg cttaccgccc ggtgctcgga gtgatccgac aggatacttg tcgccgatca
    15961 actttgagct agcaaacggt gagtccgttg ctgcatagtc actaagactc tgtccagatt
    16021 ttccgggcaa ttccaggcgg aagttccccc aagcttccgg acgcgaacga cgcgcgtttt
    16081 cgaccgaccg aagtgtgggc tctgacgttc gccgcggagc ggcttctggc tggccgggcc
    16141 atgcattggg tgaagggcgc ccgggtccgg aagggagggc gccgtgtcgg ctgacctgat
    16201 cgacttggcc gagtggcagc ggctgcggat cacgggggca ggaggcgctc gtctcaggct
    16261 ggtgctctcg cgctcgggtg cgccgagtag gatgcgtctg aggggccacc gctgcgcgtc
    16321 gttgccgcta ggctagcaga gctggcgcct ggattgggcc gcgaggcacg cggccaaggg
    16381 ggacccacgg cttgcggagg ggagccgcgc gggaggggag aagtgcgtct gcgaggacga
    16441 gcggtgacgg gccaccgggg gcgagacttg cgggcgcgct aacagcagcg gagcggcttt
    16501 ggagccacgg ttcggacggg tcgttctcgc tgacgatgca agtagcctcg tacgggatca
    16561 cggaagacgt ggtagtgtcg cccaacaggt gtcggtgcca acagttgctg tagcaatgct
    16621 gcttgctgct gcttgcagtg gcggctgcaa acacgaggac tctttccgtc cctccactca
    16681 gatgaggtcc gcggcccctc gaacagaagc accgaaggcg agccccggct ccacgtctcc
    16741 cggggacgca cagcagagtg aggcgacgac agaacacaag cgaagagtgg agttcgagcg
    16801 agccctaggc gaactgggcg gagcaactca gctcgatgac gaggtcacag gggttgctgg
    16861 gacggcgagc aaaacctggg cggtttatcg ccaagttgtc gagcgcggca cacatcgcga
    16921 gatcttagac gctttgaagc atccgaatcc cgtggttcgt gcgtacttgg ggaggcacgt
    16981 cgtagagaac ttcccgggcg cgatcgatga agttctgcct ctgctccgag acgaaacgcc
    17041 agttgacttc cagtccggct gcctcgttgt accccttcag gttgcgttaa tcgtggcgag
    17101 tgcgatctgc cacagatccg atcaagcgcc agagctccta acgctactga agcagctgcg
    17161 ctctcagcag tatccagcgg tcgtcagaaa gcgcgcgata gagtgccttg cttcgcagag
    17221 gcccagacga ccgatctccg gttcctcacc cggtccaagt gcagcccctc gttcgacacc
    17281 tagcggctga ccaaggggtt gaagatcggc atcggttctg aattttgcgc ctcggtgtgg
    17341 gggcggactg ttagtcaaaa gagatagaag gcatgcagga tgggacaagc aacaaacgat
    17401 ttcaagaatc gaaagcggct ctttatctcc ggggaaagcc gtgccgtaag gcgctgtgcg
    17461 gacgtaattg atgcttggat ttggtggagc tatgtggggc gtgccatcct ttcggagatc
    17521 ctcgatgctc ccgcgggaag gacggtagac ttctccgttg tgccggataa cttcatagct
    17581 gggctagatg ctcgcgcatc agctcgttcg ccaaacgacg cttatggtgc aggctactca
    17641 tctccggatc tcggtatcac cggcaccgga atggggtcca aggtatccgt tgtgctgact
    17701 aactcattgt cggtatcgga tggaacctgt caggctatta tgaatgttcc gtgttcgagt
    17761 ctggcagcat tcaagacaca tttcgtgttg atgcatgaga ttgtgcacgc aaaccgaatc
    17821 gctcggggca agctgcggac agtttcaatg ggcggtaggt tgaagaatag cgaggaggcc
    17881 atcgctatca tagttacgaa catgctaatg tcggaaaaag gggaatccgg gttacgcaaa
    17941 acctatgata cctcggatgt gctgacgatt actgctgatg agttcgtgaa gcaagatggg
    18001 gtggagagct tggttcgcac catcgccaat gatcatcctc gattgaagac aacgctcaat
    18061 agaaaggaga tcccagtgcc gttcaatccg atctactctg tctttcaggg agcgccgtaa
    18121 gcataccaga ccagggccct ctgccactcg tgacactcag accgagttca gttcctagtc
    18181 atcatcctgc ttgaggaagc ccggcaggcg ccggagctgg cggtcctttc gaccatggcg
    18241 catggcgaac accgcagggg tctgatcgcc atgatggcgc agacgttgcc gaaccccgcg
    18301 cagcctcagg acgtgagttg agccgtgtgt cgcgaaagcc tgccatgcca accctcgacg
    18361 ggcgcataga gtacgtggag aacacgacgg cgggctgggt tggcagcgcg cggtgaccgg
    18421 tgcacgagca acggcttcat caggagcacg tcaccggcgc ggcaatcgta gtagcgaaaa
    18481 gcttccgctg gaagctggcc gagtttcacc atcggacaag cgcccgcgtg cgtggcttcc
    18541 aggagccacg gcgaggcctc cgctgttcgc gtcggagtca tcgaagtgaa cacgaaccgc
    18601 gacgagcctc tcgagaacac gaagtggcgg ctcgccgtat gtcatcccgt ggcgagttcg
    18661 gacgttgcgc acgcaggatg aactcgcgtt ggagggcaca ggaacaatca ggtcctggcg
    18721 ggcgggaacc gcccagttcg caccggagtt cttgtcgaag aacagcgtca atcggaaacg
    18781 ctggctcgct cagggcgcgc gcggcacctc tacggccgac gatctcggca ccgctaagtg
    18841 aagtcacgga ggtcatcggc ggggacgggc gctgccacct gcggcgggcc ccactcatgg
    18901 ggacgggacg tcaggggcga gagggcgccg ctttcgctgg atgaccaccc cagtttgaag
    18961 aggtggcttc tctgccgcgt tgacggtgtg cggcgcgcgc ggtgggctcg acggaccctg
    19021 ggctcgaacg gctgcgctcg cgtgtcactc cgacgggtgg tcgcgctggt tatcttgggc
    19081 ccgccaccaa acaggcaggc acaaatgaag aaagccgcca aaggggcggc tttcaatgcg
    19141 agaattcttt gagccgactg ttgttgaggt cgtctttgag ccgactgtgg gacttgaacc
    19201 cacgacctgt gctttacgaa agcactgctc taccgctgag ctaagtcggc cttggcgttg
    19261 gggcggcgac actaccaaca atttttggac gttcaagagg gatttgcggg ttgccggccc
    19321 gggacttcac ttttttgaag gtcgaaccag ggtcgaggcg gtgggtcgag gcggtgggcc
    19381 ggcccgcggg ggactgctgt tggggccggg gagagcgatg agttctgagt gcggcccgct
    19441 cggcggcggc gctcgccgac gggtgcagcc tttgcttttg ctaaaccgag ctctccgggt
    19501 tcgagacgct agtcaccgcg aagacgccgt gcacttcggc tgcggttgag ggcgtggggc
    19561 ttacctcggt gcgctgctgc aaacgacgcc aggctgagct gaatcagagt ttttgagaga
    19621 gtaaaccgaa ctcggatgtg gcactcaatt cgccttcgcg cgactgccct gagccgcgag
    19681 gcgggcgcac tcttgttcgg cgggggtgtc cccgaggcga aggttcttca gttcttcggc
    19741 gggggcgccg gggagttcat tggctagggc ctccagcgcc tgatcacatc gcttgttggc
    19801 ggcgaggctc gcggcgtaga ctcgcaggct gaaaacgctc ggataactca gacgaatgga
    19861 gaggtcggcg tccgctaatg cgctctccgc acggccgtta aggatccggg ctgctgcgcg
    19921 ctgtatgcgc gctgaaggat tgaacgggtc gagcgccacg gccttgtccg ccatcttcag
    19981 ccgcacggcg tcgtctgtgg agagcatgca cgccatgtcc cagcttcgcg agctttccgg
    20041 atgtgctttc agtgctttgt cgaccaacgc ttgccgctcg gtcttgggga gacccccgta
    20101 gtggaacaaa gcggccaagg acagtatctc gttgggatcc tcgatcagcc ccgcacgcgc
    20161 ttcttccaat gccaattgtg agtccgctgc ggcggttgcg aacaagccgt gtgcttgagc
    20221 ggaggtgata ggggtgatgt gcacgtcagg gttgattcgg gagacctccc gctgcacgag
    20281 cgtaaacacg cctttggtcg cgagctcttt gcccagccgc tcgggcgtaa cgtccgacag
    20341 atgttgcgcc caggcggcac gccagggctg gagtcgcgag agcccgcgct gaaactgggc
    20401 gaagtcgtcc tcgtgacgat tcaccagatt agtgaagatg ccgtaagcac cgatgtagaa
    20461 gccgtatccg cgcacgctgt ggtcgtaagc tttctggtcc aggatgcctt gaatactcag
    20521 gtcagtcgcc aaagttctgg cttctttgac gttcggcggc atgagcccga gcgtagcttg
    20581 accggtttgc ggatcgtacc gcacggtttc cagaaaggtg gccaggccct ccgcaaacca
    20641 aagaggttga ttgggcatgt agaagtggct caggtggtga gccatctcat gcgaaatgaa
    20701 tccctcgata cccttgcttc cgggcgaaaa gacgagtcgg tctccaaacg gaatggagcg
    20761 cctgaccata gcgagggcgc ccggcggcaa gaagcgaccc gcgtccgtcg gcctccgaaa
    20821 catgagcacg accgtgggcg ccggcggcgc tttggagttg ccccaaatca gatccaacat
    20881 cgctgaccgc gtctgcgcta gggaacgcgc ccaatccagc gcgccttggc cttgcatgtc
    20941 ggtgtagacg acgacgtttt cgccatcgaa ctcgatcttg cgccatgact gagtggcgct
    21001 ggcgggggcc gccgcaggtt gcatgctgca cgcggtgcag agccagccga ggccgagggt
    21061 gaggagtcgc ttcaaggctg cggtccgagt catggataac agctttgagt gatactctgg
    21121 ctctcgcgcg ctgagaagtc cccgaacggc gatacagact tctgtctgcg caaggttgct
    21181 tgcgttatgg ccgctcgcga gttcgctgtc tcgattcgcg cggctgtttc gattcgcgcg
    21241 gctgtctcgc gatgaagcga gtgggcccgg tgctgtgggt gcaccgtaag acaaactaaa
    21301 tgtatcccag gtcttgcccg tggcgattga ccaggttggt gaagatgcca taggcgccga
    21361 cgtagaagcc gcacccgggc gtgctcgggt cgacttcttg ctgatcgaga atgccttgaa
    21421 tgctctggtc ggccgccacg gtgatggctt ctttgacgtt ccacggcatg agccctagcg
    21481 tggcttggcc ggtttgcggg tcgtaccgaa cggtttgcag aaaggtcgcc aagccttccg
    21541 agaaccattg gggttgattg ggcatgtaga agtggctcag gtggtgggcc atttcatggg
    21601 aaatcacctc ttgtgaggct ttcccttcag gtgagagcac gatgcggtcg ccgaacggaa
    21661 gcccccgact gaccatggcc cgcgcgcccg gcggcagaaa gtgccgcgca tccatcggcc
    21721 tgcggaacat gagcacgacg gtgggagcgg gtggcgcttt ggacttcgac caaatcaaat
    21781 ccaacatcg
//
LOCUS       DASTKU010000565         4249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_110401, whole genome shotgun sequence.
ACCESSION   DASTKU010000565 DASTKU010000000
VERSION     DASTKU010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110401"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VFQ61_19655"
     CDS             <1..1365
                     /locus_tag="VFQ61_19655"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9956725.1"
                     /translation="RGFRHFGVIGGAGMLLCWLSAYGILPAVLVLSERLSPLDVRASL
                     RDRLAGLYGRPFAWLAKRYSAPVAIFGIALGVLASVCSALYFVGDPMEYNLNNVLNRE
                     TTPTSAGRLSSRVNRVAGRLNQGGRAILVDRLDQVELVTRELEKRRDSAPTGKKPFGD
                     VLSILSLLPRDQPRKIELLTDIVDRAQRAHRRGVIDEHQWQEIEPQLPKDLRPIGVAD
                     LPEVVARPFEERNGDRGKIVYVAPADGRSLNDAHYLMEWANSFRTLELPNGEIIRGTG
                     DAVVFSDMLINIREDAPRVAFTSLSGTLLVILLAFRGRRAGVIALVTLLLGISWLLGT
                     LYISDTKLNFLNFVALPIAIGAGADYAINIMKRRELEGSAGIERAFIETGGAVVACSM
                     TTLCGYLALLLSLNGAVRSFGLAAAVGELATQLAAMVVLPAVLFWHARQSPTPAARPE
                     VLGQ"
     gene            1517..3451
                     /locus_tag="VFQ61_19660"
     CDS             1517..3451
                     /locus_tag="VFQ61_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET9956726.1"
                     /translation="MSRLQYSVLRRLVKLFRWPGRSPLGRLLDQAAPLDLRILGRTLL
                     HAALVGIAAGLAGAILFVGLEHFQRLVLEVLVGYTPLRADGETFHVSDEPRVFRPWLL
                     VLAPALGGLACGLVTLLAPEVRGGGGDAMIEAFHRGGGSIRPRVIVVKALASMFTLGT
                     GGAGGREGPTMQIGGALGSLVARMLSVSVRERRILMIAGVAAGISAVFRTPLGAALLA
                     VEVLYKDGFESEALIPSVLASVVSYSVTTTLGGQGTLFTRVPRFPFHPEQLLLYLLLA
                     LVVAALAVVFLEVYGSVKGFFYKLPIPAWSQPGLGGLMLGVFATPLVWAVGVHIHQPG
                     QGLGLLGGGYGAVQMAIAGSEFLPEGWTAVVLLLLLAIAKLIAASITIGSGGSAGDFA
                     PSLAIGGLVGGAFGRAAQILLQDPRIIPGAFALVGMGAFYGGIAHVPLSALVLVCELA
                     GNYDLLVPLMLTQGIAFVALRQRALYHAQVATQRESSAHRDHLLLSFLETLQVRAVVN
                     EQARPVCFHRETPLSEMVQHLTHSGQQDVFPVVDSDNKLAGLVTSSTIHVALGEMADA
                     AWVLAADVMQADVSVTFDDDLRTASERLVSNELREVPVIGRDRQVVCMLKETDIARLY
                     VSAAARADVDRRAVRRGRGA"
     gene            3677..4036
                     /locus_tag="VFQ61_19665"
     CDS             3677..4036
                     /locus_tag="VFQ61_19665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9956727.1"
                     /translation="MKTVLVVEDTTDMRELLVDVLETEGYRVLAASHGQEALETLHAC
                     SEKPAVILLDWMMPVMDGPAFLQALYKEPEFSSVPVVVVSAIASLIDVKGVARVFEKP
                     VRFPELRRVVRDLCGTP"
BASE COUNT          693 a         1305 c         1395 g          856 t
ORIGIN      
        1 cgcgggttcc ggcacttcgg cgtgatcggc ggtgcgggca tgctcctgtg ttggttgtcg
       61 gcctatggca tcctgcccgc cgtgctggtg ttgagcgaac gattgagtcc tctcgacgtt
      121 cgagccagcc tgcgtgatcg actggcaggg ctttatgggc gaccgttcgc ttggctcgcc
      181 aagcgctact cggccccggt cgccattttc gggattgcgc tgggcgtgtt ggccagcgtc
      241 tgttccgccc tgtacttcgt cggtgaccca atggagtaca acctgaacaa cgtactgaat
      301 cgggaaacca cgccgacgtc cgccggacgc ctcagctcgc gggtgaatcg cgtcgcaggg
      361 cgcctgaacc aaggtggacg cgccattctc gtggatcgac tggatcaggt agagctcgtc
      421 acccgggagc tcgagaaacg tcgggactcc gcgccaaccg gcaagaagcc gttcggcgac
      481 gtactcagca tcttgagttt gttgccgcgg gaccagccgc ggaagatcga gctgctcacc
      541 gatatcgtcg accgggccca acgcgcgcac cgccggggcg tgatcgacga gcaccaatgg
      601 caggaaatcg agccccagct gcccaaagat ctgcggccaa tcggggtggc ggatttaccc
      661 gaggtcgtcg cgcgtccgtt cgaagagcgg aacggcgatc gaggcaagat cgtctacgtg
      721 gccccggccg acggccgcag cctgaatgac gcgcactatc tgatggagtg ggccaacagc
      781 ttccgcaccc tcgagctccc caacggcgaa atcatccgcg gcaccggcga tgcggtggtg
      841 ttctcggaca tgttgatcaa catccgcgag gacgcgccgc gcgtcgcatt cacgagcttg
      901 tcggggacgc tgctggtcat cttgctggcc ttccggggca gacgcgcggg agtgatcgcg
      961 ttggtgacat tgctgctcgg gatctcttgg ttgctcggca cgctctacat ctccgacacc
     1021 aaactcaact tcttgaactt cgtggcgctg ccgatcgcga ttggagctgg cgcggactac
     1081 gcgatcaaca tcatgaaacg ccgtgaactc gagggctccg cggggatcga gcgcgccttc
     1141 atcgagaccg gaggtgccgt cgtggcctgt tccatgacga ccttgtgtgg atacttggcg
     1201 ttgctattga gtttgaatgg cgccgtgcgc tctttcggcc tggcggcggc ggtgggggag
     1261 ctcgccactc aattggcggc gatggtggtg ttgcccgcag tactgttctg gcacgcgcgc
     1321 caaagcccca cgcccgctgc acgtccggag gtgctggggc aatgagccca gctggtcttt
     1381 agcgcgagcc gcacggagcc agctctgccc cgcgggctgg ctcgacggca cacggcagtt
     1441 acttcggagg caatcgctgg ccaccgcctt ttgcagccgt gcccaatcga gactagagcg
     1501 cggggagcga atgtcgatgt cccgcctgca gtactctgtc ttgcgccggt tggtgaagct
     1561 ctttcgctgg ccggggcggt cgccgcttgg ccgcttactc gaccaggccg caccgctgga
     1621 tctgcgcatt ctgggtcgaa cgctgttgca cgcggccttg gtcgggatcg cggcgggctt
     1681 agcgggagcc atcctgttcg taggcttgga gcacttccag cgcctggtgc tcgaggtttt
     1741 ggtcggttac acgccgctcc gggcggatgg tgagacgttc cacgtcagcg acgagccgcg
     1801 tgttttccgc ccctggctgt tagtgctcgc cccggcgctc ggaggtctcg cctgcggtct
     1861 cgtcacgctc ttggcccccg aggtcagagg cggcggcgga gacgcgatga tcgaggcatt
     1921 tcaccgcggc ggtggctcga tccgaccgcg cgtcatcgta gtcaaagccc tagcctcgat
     1981 gttcactttg ggaacgggcg gcgcgggcgg ccgcgagggg cccaccatgc aaatcggcgg
     2041 agcactcggc tcattggtag caaggatgct gagcgtaagc gtccgcgagc gacgcatcct
     2101 gatgattgcc ggcgtggccg cagggatctc cgccgtgttc cgaacgccgc tcggcgcggc
     2161 gctactcgcg gtagaggtgc tctataaaga cggattcgag tccgaagccc tcattccttc
     2221 agtgctggcg agcgtcgtct cgtattcggt gactaccacg ctcggcggac aaggcacgct
     2281 attcacccgc gttccgcggt tcccgtttca tcccgagcag ctgctgcttt acctcctctt
     2341 ggcgctcgtg gtggccgcgc tagcggtggt gttcctggag gtctacggca gcgtcaaagg
     2401 cttcttttac aagcttccga ttcctgcttg gagccagccc ggactcggtg gtttgatgct
     2461 gggcgtcttc gcgacgccgc tggtgtgggc ggtcggagtt catatccacc aaccggggca
     2521 aggtctgggg ctactcggtg gcggatacgg tgccgtgcag atggccattg cgggctcgga
     2581 gtttctccct gaaggctgga ccgccgtcgt gttgctcctc ttgctcgcga tcgcaaagct
     2641 aatcgcggcg tcgatcacga tcggctccgg cggcagcgcg ggtgatttcg ccccctcgct
     2701 cgcaattggg gggctcgttg gaggcgcttt tggccgcgca gctcagattc tgctgcagga
     2761 cccgcgcatc atacccgggg cgtttgcgtt ggtgggcatg ggagcgtttt acggcggcat
     2821 cgcccacgtt ccgctcagtg cgctggtgct cgtgtgcgag ctggccggca actacgattt
     2881 gctggtgccg ttgatgctga cccaagggat cgcgttcgtc gcgctgcgcc agcgcgcgct
     2941 ttatcatgcc caagtcgcca cgcagcgcga atcctcggcg catcgagatc acctgctatt
     3001 gagcttcctg gagacgcttc aggtcagagc cgtcgtcaac gaacaagctc ggccggtatg
     3061 ttttcatcgt gagacgccgc tctccgagat ggtgcaacac ctcacccact cgggtcagca
     3121 ggacgtgttt ccggtcgtag attccgacaa caaactcgcg ggcttggtta cctcatcgac
     3181 catccacgtg gccctcggcg agatggctga cgcggcttgg gtgctggcgg cagacgtgat
     3241 gcaagcggac gtcagcgtca ccttcgacga cgacctgaga acggcaagcg agcgcttggt
     3301 atcgaacgag ctgcgcgaag tgcccgtcat tggccgcgat cgtcaggtcg tttgcatgtt
     3361 gaaggagacc gatattgctc ggctttacgt aagtgcggcc gcacgagccg acgtagatag
     3421 gcgagccgtt cggcgcggac gaggcgcgta aagctccggc gcgcgacaga gtgcggcacg
     3481 cggcaaatca ccgcatacag agcccggcca cgcaacggct ggccgggcgc gaaatcactc
     3541 agtggcttcg agcgccgatt acgggccagc ctggcgcgcc caaccgacgg gtgatgtagc
     3601 ccagcatcac accgaaacaa gttatcgcca cacgccaggt tcacgaacac ccgcgcccga
     3661 gtgctacttt gaaatcgtga aaactgttct cgtggtggaa gacaccacag acatgcgtga
     3721 actcctcgtg gatgtcctcg agaccgaggg ctaccgggtg ctcgccgcat cgcacggcca
     3781 ggaggcattg gaaacgctac atgcctgcag cgagaaaccg gcggtgatct tattggactg
     3841 gatgatgccg gttatggacg ggccagcctt cttacaagct ttgtacaagg aaccggagtt
     3901 tagctcggtg cccgtggtcg tcgtctcggc catcgcgagc ctgatcgacg tcaaaggggt
     3961 tgcccgtgta ttcgaaaagc cggtgcgatt ccccgagctc cgcagagtcg tgcgtgacct
     4021 gtgcggcacg ccctgaggat ccgcactggc cttgaatcag ggccgtccag tagggttcga
     4081 gagtgcgcac gcttttgacg gttatcctgc gatggctcgg caaacacgag ctggggtcct
     4141 tgcttactgt ctccactctg gccggaggca gtttggcctt cgcgaccatc gcctccaagg
     4201 ttgcgggagc caagccgggt gatttggatc gcgaactctt gctctcgct
//
LOCUS       DASTKU010000566         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1450085, whole genome shotgun sequence.
ACCESSION   DASTKU010000566 DASTKU010000000
VERSION     DASTKU010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450085"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VFQ61_19670"
     CDS             <1..475
                     /locus_tag="VFQ61_19670"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET9956728.1"
                     /translation="PTLEVIRRVSAEPRVLLTSFYSYVTCRARLAGYPGPRGLGREEV
                     LFAASLSKLLTSKLFRGRALTGIKGVYSGCRIQIPTAAPGMALDEPDLIRAFHDLGLA
                     VDYWVINDRTRAQQLLERGADGIITDDPRALRSCFEAAAETAAWRARHTQTPVSI"
     gene            1107..1814
                     /locus_tag="VFQ61_19675"
     CDS             1107..1814
                     /locus_tag="VFQ61_19675"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET9956729.1"
                     /translation="MPANPASPGDSAEPRDLRNTAHPARNSACNEQAPQPFLVAAHFD
                     AHAAMRPGQAAEFQELLKRAIRYRTEQYGYFEGYGTRAWNARAPGELARVTTFFGVPV
                     NIHERIVPVLRCVEQAITELCPEKYRPVVLSGIRKRNTYFNGEVSNHVYGIALDIDPT
                     LNPCCGCVEPWRSNPRCGAGKNKYERMAMPRCWVAQFERFGFYWLGNAPMEDTMHFEF
                     LGDPARIPSSDSRPRAD"
     gene            1920..2582
                     /locus_tag="VFQ61_19680"
     CDS             1920..2582
                     /locus_tag="VFQ61_19680"
                     /inference="COORDINATES: protein motif:HMM:NF016932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HET9956730.1"
                     /translation="MKELLKRVIAEREGRVYPELFGEVSKNIYTLDEEFFQRVFEQKA
                     EPEWLLTGVLECPPSSKHKDYIYVSSGLSNPVVEQAEPMSPEDPSWLGVEVVFRTTEP
                     GPWAVELVKRVAAFEILLAYQRFEGRDRLAPGARIPIGNPITLSSPSELTWLLVVPTK
                     FEPPAFQLESGVVEFLQVIGITAAEAKLAREQGPEMLFDLLGAHGALSVTDPKRGTHA
                     TE"
     gene            complement(2622..>4517)
                     /locus_tag="VFQ61_19685"
     CDS             complement(2622..>4517)
                     /locus_tag="VFQ61_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956731.1"
                     /translation="LEPAPTAATAWRADWSEEQSASEFVAAGQPANAQDLVPPSELEV
                     EFSPQPAAVTNDLAAGQDLSAVQPTAHEGVSALPSTAVVEETARYVPLEGSSLESDQS
                     SPREDLVTTESLPKLAPVESRGELTSDAGFSSDPIVSEAAPPEPLLDRPEPLPATTES
                     LERTEPLPARAESLASPSEKTATAAGVESHDATVFASSSELAPPAKPAVSLDLERDID
                     WEEAGRVSKPFGAFPQVPVSSSLPDSRAASSNLQARGADAGAETSRTEPTSGAAEIAP
                     ISSIRSKPPGPEERRSQLPRARTPRGERAARSDHSERVPQATLRRPRSLAAPAREPAE
                     SVAAKTPPAPPRASLAQSARLLAALALGVCIGFGLGQLVARKSADKQLSPGAPAPALD
                     IRSIQGESESNPAPVPVPPAPVPGQPNEGVPAEAQAAVPRASDPSGMLEPQPVPDSEA
                     AAVDEGRGVNRAMEASPSEARRSAVLPAGPAGRQPSVNLPPRGPKKGEPSSLPAAAPA
                     PGVIEPEVSEGKQLETEAAEAGSAASAAKLLAARAPFDPKLANSALAKAASAFGACVK
                     SGESGGVAVVTVTFSPSGKVSLTQVAGSRFAGTPAADCISAAARNAQIPPFLGKPVSV
                     KKSFVVE"
BASE COUNT          771 a         1465 c         1432 g          849 t
ORIGIN      
        1 tccgacgctc gaggtcatcc gacgtgtcag cgcggaaccg agggtcctgc tgaccagctt
       61 ctactcttat gtgacgtgtc gagcccgctt ggcgggttac cctggcccgc gcggcctcgg
      121 tagagaggaa gttcttttcg cggcctcgct ctcgaagttg ctgacttcca agctgtttcg
      181 cgggcgtgcc ctgacgggta tcaagggcgt gtactcgggc tgccggattc agatccccac
      241 cgccgcgcca ggcatggcgc tcgatgagcc ggatttgatt cgagcctttc atgacctcgg
      301 gctggcggtc gactactggg tcatcaacga ccggacccgt gcgcagcagc tcctcgaacg
      361 aggcgccgac ggtatcatca ccgatgaccc ccgcgccctc cgcagctgct tcgaagctgc
      421 agccgagacc gcagcctggc gcgcgcgtca cacgcaaacg cctgtttcga tctgagccgt
      481 cagcgcaagc ccgcaggctc cacgaaagat ggcgtagttc agacacctaa atcttcggga
      541 tggacctctt cgccaacgaa tagtctactc ggccaaggta cgcgccgccg tgcccatgct
      601 cgttcttgtc tgtacctaag tctgtagctg attcgcgcgt gctcccttgg cctgatatgt
      661 ccccttcgga agcgtcccct actgggctgt cccctactgg gctgtcccct actcggctgt
      721 cccctactcg gctgactacc caattcaaga cttcagcgtg tgtcgagcgc cggctcgtgc
      781 gcgatgactg tattcggcgc aactgcgcgc tcgttgcaag cttgctggtg ctgagcctga
      841 acgccggctg tcggaagcat ccgcgaccta tcgccaatgc gcacgcttcg gaactggcgc
      901 cgaatacgcg gacgtcccca cgcgtgcgac gcccagaacc gcccgccgag gcaacgccgc
      961 ccagcgtcgc tctgaatgct tcccgagccg ctccgctcgt cgttgcaagt tcgttccccc
     1021 tcgcttcgag cgagccgaac tcgagtttgg cccccacttc gagcctccct cgagggcccg
     1081 aaccgccgaa ctccccggag cctgcaatgc ccgccaatcc cgccagccca ggagattcgg
     1141 ctgaaccccg cgatctgcgg aacacggcgc acccggcgcg caactcggcc tgcaatgaac
     1201 aggcgccgca gccatttctc gtggccgccc acttcgatgc ccacgccgcg atgcgaccgg
     1261 gacaggctgc agagtttcaa gaactcttga agcgagcaat ccgttaccgc accgaacaat
     1321 atggctactt cgaaggctat ggcacgagag cctggaacgc ccgggcaccg ggagaactgg
     1381 cgcgtgtcac gaccttcttt ggggtacccg tgaatataca cgaacggatc gtgccggtac
     1441 tgcggtgcgt ggagcaggcc attaccgagc tctgcccaga gaaatatagg ccggtggtgc
     1501 tctcgggcat tcgcaagcgc aacacttact tcaacggcga agtatcgaat cacgtctacg
     1561 gcattgcctt ggatatcgat ccgacgctga acccgtgctg tggctgcgtg gaaccgtggc
     1621 gtagcaaccc gcgctgtgga gccggtaaga acaaatacga gcgcatggcg atgccccgtt
     1681 gctgggtcgc gcagttcgag cgatttggct tctactggct gggcaacgcg ccgatggaag
     1741 acacgatgca cttcgagttt ctcggcgatc ccgcgcgtat cccgagttct gactcgcggc
     1801 caagggcgga ttgataaccg gctggtagcg cgctgggcaa cgcctacgct ttttgttcgg
     1861 ggatggtttt ggggctcgca atcgagccga gatcgcgtat ggtcgtccag gtggttgtga
     1921 tgaaggaatt actcaaaagg gtcatcgctg agcgggaggg ccgggtttat ccggagctgt
     1981 tcggtgaggt gagcaaaaac atctacacgc tggacgagga attctttcag cgcgtgttcg
     2041 agcaaaaagc agaaccggag tggctgctga ccggcgttct ggagtgcccg ccttcgtcga
     2101 aacacaaaga ctacatctac gtgtcttcgg ggctctcgaa cccggtagtg gagcaggcgg
     2161 agccgatgtc acccgaagat ccttcctggc tgggggtcga ggtagtgttc agaacgacag
     2221 agccagggcc gtgggcagtc gagctggtga agcgcgtcgc agcgttcgag attctgctcg
     2281 cgtatcagcg cttcgaaggg cgagatcgtc tggcgccggg cgcgcgcatt ccgatcggga
     2341 atcccatcac gctgagctcg ccgtcggagc tgacgtggtt actggtcgtg ccgaccaagt
     2401 tcgagccccc ggcgtttcag ctcgagtcgg gggtcgtcga gtttctgcaa gtgattggga
     2461 tcaccgctgc tgaagccaag ttggcacgcg aacaagggcc ggagatgttg ttcgatttgt
     2521 tgggcgcaca cggcgctctg agcgtgacgg atccgaaacg cggaacgcac gcgacggagt
     2581 gagcacgctc ggcggcgccg agtgatctca cgagcgacac ctcactcgac cacgaaggat
     2641 tttttgacgc tcacgggttt gcccaggaac ggcggaatct gggcattgcg ggcggcggca
     2701 gagatgcaat ctgccgccgg cgtccctgcg aaacggctgc ccgcaacttg agtcaggctt
     2761 actttgcccg aaggcgagaa ggtcacggtg accaccgcca ctccgcccga ttctccgctt
     2821 ttcacgcagg cgccgaaggc actcgctgcc ttcgccaacg cgctgttggc caatttcggg
     2881 tcgaacggag ctcgcgccgc gagcagcttc gccgcagaag cagcactgcc tgcctctgcg
     2941 gcctccgtct cgagttgctt gccctcgctc acttcgggct cgatgacacc gggcgcgggt
     3001 gcggccgctg gtagcgatga gggttcgccc ttctttgggc ctcttggagg aagattcacc
     3061 gaaggctgac gaccagctgg ccctgcaggt agaactgctg atcggcgcgc ctcggaaggt
     3121 gaggcctcca ttgccctgtt gaccccacgc ccctcgtcga cggccgcggc ctccgaatcg
     3181 ggtactggct gaggctccag catccctgag ggatccgacg ctcgaggcac cgccgcctgc
     3241 gcctctgcag gaactccttc attcggttga cccggtaccg gcgctggcgg cactggaacg
     3301 ggagcagggt tcgactcgga ttccccttgg atactgcgga tgtccagggc cggagcaggc
     3361 gccccgggag aaagctgttt gtcagcagat ttccgcgcta ccagctgacc gagcccgaat
     3421 ccgatacata ccccgagcgc caaagccgcc agcaatcgag cgctctgcgc aagagacgca
     3481 cgcggcggag ccggaggcgt cttcgccgca acgctctcag caggctcacg cgcaggtgcg
     3541 gccaaggatc ggggccgacg cagggtggcc tggggaacgc gctcggaatg gtcgctgcgc
     3601 gcagctcttt cgccacgcgg agtacgtgcg cgcggcagct gcgaccggcg ttcctccggg
     3661 cctggcggtt tgctacgaat gctggaaatc ggcgcgatct cggccgcacc cgaggttggc
     3721 tccgttcgtg atgtctcggc gccggcgtcc gctccccgtg cttgcaggtt cgagctcgcg
     3781 gcgcgtgagt cagggaggct cgaagagacc ggaacctgcg ggaatgctcc gaagggcttg
     3841 gaaactcgac ccgcttcttc ccagtcgatg tcccgctcta gatccaagct caccgccggt
     3901 ttggctggcg gcgcgagctc cgagctcgac gcgaagactg tcgcatcgtg actctccacc
     3961 cccgcggcgg tggctgtttt ctcgctcggg cttgccagcg actcggccct cgccggcaac
     4021 ggctcggtcc tctcgagcga ctccgtcgtt gcgggcagtg gctcgggccg gtcgagcagc
     4081 ggctccggcg gggcagcttc cgagacgata ggatcgctcg aaaagccggc atcggaagtc
     4141 agctcgcccc tgctctctac gggtgcaagc ttcggcaagc tctccgtcgt aacgagatct
     4201 tcacgaggcg aactttgatc gctctcgagc gacgagccct cgagtggcac gtagcgcgcc
     4261 gtttcttcca caactgccgt cgagggcaga gcgcttaccc cctcgtgcgc cgtcggttgc
     4321 accgccgaca ggtcctggcc cgcagcgagg tcgttcgtca ctgctgcggg ttgcggagag
     4381 aattcgacct ctagctccga aggcggcacc aggtcctgcg cgttcgcagg ctgccccgcg
     4441 gccacgaact cggacgcgga ctgttcttca ctccagtccg ccctccacgc ggtggccgca
     4501 gtcggggctg gctccaa
//
LOCUS       DASTKU010000567         3209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_556535, whole genome shotgun sequence.
ACCESSION   DASTKU010000567 DASTKU010000000
VERSION     DASTKU010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556535"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFQ61_19690"
     CDS             complement(<1..555)
                     /locus_tag="VFQ61_19690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956732.1"
                     /translation="MGSVPVGGTLRCERCGAEFASWPTSERARCSSCQHEQAVSPSLR
                     GDLARYSEHVAGEHARTREAEREAVTWEHWADQYSLSKSQPWMLPLVVATVVPVISIV
                     VALVAHQLAWLGGAGVEVVGAIAACLSWVGAAAYVVVYFAKQRRAPREAVIPQVRVAC
                     PICGAPGELAVGQPARACRFCRGAV"
     gene            791..1147
                     /locus_tag="VFQ61_19695"
     CDS             791..1147
                     /locus_tag="VFQ61_19695"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET9956733.1"
                     /translation="MVPSKQSADVLVFIRTTLRPGCDLTAYEVMDARMNEILSKIPGY
                     LGVKGYTADDGESIAIVRFESREALIQWRDHPEHLEAQRAGREKFYATYEIRVCAVER
                     AYDFSAEREPLRISRG"
     gene            complement(1176..2528)
                     /locus_tag="VFQ61_19700"
     CDS             complement(1176..2528)
                     /locus_tag="VFQ61_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956734.1"
                     /translation="MSSSSIRLARDEYHRALSEAALDRTRTIVATARADPGLTSAGHG
                     IDRILRPCFISAAQHAAAQQSAVAVVAALESLYRAARGSAELRRKLGVATWQDQLIGV
                     EPADAPPAVGRLDGFLGRDDQLRMIEFNPAPGGTYEVHALSRLFAATPAMDALSRYAP
                     HWPDPLEAAYQMLLANYARADRPTSVNVAVLGWSQAPNIGEIQRLAAHLTQRGMTIKP
                     VQADDAWVLDSGGLSLDAFRVDLLLFLDPMQLGDFLSRFGPAHPVFRAVREGHSSFFG
                     GLFRTLFLASKRVFAALSEPTFRALIPPELHAELHKCIPWTRIVQEGRTTHASGEVDL
                     LEFAAKHRTQLVLKPALASGGKGVVLGWRTSVDEWEQALVQSLADGGVVQERVDAPQD
                     SFPLWEDGKLVFRELFWDLNPFVWRGRTAEGYLVRVGSDPLLNTSAGLGSSTALFVLG
                     "
     gene            2598..>3209
                     /locus_tag="VFQ61_19705"
     CDS             2598..>3209
                     /locus_tag="VFQ61_19705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956735.1"
                     /translation="MTANSRASKTSGFHIELCGAERERAAIRAFREDICRTIRLEVGG
                     PAAEPLDYGSYLFLFAEGETQPAGMVEFFFYDQAFAAFSDAVYSQAADLGTYGSLADL
                     VHLRSVILEVGIRHTRVFVYLMAATVLVTQRLGATHLTAGTGIHNDAILALHRRAGMT
                     PLGRYVVDGAPQQLSLLALAPLLQRAKGLLGRGGITVNDSAAFE"
BASE COUNT          616 a         1038 c         1022 g          533 t
ORIGIN      
        1 cacggcgccg cgacaaaagc ggcaagctcg tgcaggttgt ccaaccgcga gctcacccgg
       61 cgcgccgcaa attgggcacg ccacgcgcac ctgagggatc accgcctccc ggggcgctct
      121 tcgctgcttc gcaaagtaga cgacgacgta cgcggcggcg cctacccaac tcaagcacgc
      181 ggcgatggcg ccgactactt cgacacccgc tccacccaac catgcgagtt gatgcgcgac
      241 gagcgcaacg acgatcgaaa tcacagggac gaccgtcgcg acgacgagcg ggagcatcca
      301 gggctggctt ttcgagagcg aatactgatc ggcccaatgc tcccaggtca cggcttcgcg
      361 ctcggcttct cgcgtgcgcg catgctcccc cgccacgtgt tcgctatacc gagccaggtc
      421 tccacggagc gacggactca ccgcttgttc gtgctgacaa ctggagcagc gggcccgctc
      481 ggaggtcggc caggatgcaa actcagcgcc acagcgctcg catcgcagcg tgccacccac
      541 gggaaccgag cccatgcgtt ctaccctatc agtccttgcc ttcgcatcaa ggcgcgactt
      601 caggtccagt ccgttggacg cggactcatt gccgcaacaa cgtgctagcc gcgggtgaga
      661 cggcggagct cgtgcaaagt gtcaaccgag acggccactc tccgtcgcgc ttaccggaac
      721 tcacgcttcg cgcttacggg aactcacgcg cgtgtaaccg acccgtgggt acgaacgtac
      781 tggaggggcc atggttccct cgaagcagtc agccgacgtc ctcgttttca ttcgcaccac
      841 cctccgtccc ggatgcgatt tgaccgccta cgaagtgatg gacgcgcgaa tgaacgagat
      901 cctgagcaag atccccggct atctaggcgt caaaggatac accgccgacg acggggagag
      961 catcgcgatc gtccggttcg agtcgcgcga agcactcatc cagtggcgcg accaccccga
     1021 acatttggag gcgcaacgcg ccggtcgcga aaagttctac gcgacctatg agatccgcgt
     1081 ttgcgccgtc gaacgtgcct acgacttttc cgcggagcga gagccactgc ggatttcgcg
     1141 cggctgagcc acgcgtggtc gagcttgcac acaatctagc cgagcacgaa cagcgccgtg
     1201 ctcgagccga gaccagcgga ggtgttgagc agcgggtcgg accccacgcg aacgaggtag
     1261 ccctctgccg tccgaccgcg ccaaacgaag ggattcaggt cccagaagag ctcgcgaaag
     1321 accagcttgc cgtcttccca gagcgggaac gagtcctgcg gggcgtcgac tcgttcctgc
     1381 accacaccgc cgtcggcgag ggactgcacg agcgcttgct cccattcatc gacggaggtg
     1441 cgccacccga gaacgactcc cttgccaccc gaggcgagcg cgggcttcag cacgagctgc
     1501 gtgcgatgct tggccgcgaa ctcgagcagg tccacctctc cgctcgcgtg ggtcgttcgg
     1561 ccttcctgca cgattcgcgt ccagggaatg catttgtgca gctccgcgtg gagctcaggc
     1621 gggatcaagg cgcgaaacgt tggctcactc agggccgcaa agactcgctt ggaagccaag
     1681 aagagcgttc ggaagagccc gccgaaaaag gagctgtgcc cctcgcggac ggccctgaag
     1741 acagggtgag caggaccgaa gcgcgaaaga aaatcgccga gctgcattgg atcgagaaac
     1801 agcaatagat cgacacgaaa agcgtcgagc gaaagccccc ccgagtcgag cacccatgca
     1861 tcatcggctt gcaccggttt gatcgtcata ccccgttggg tgagatgggc tgcgagccgt
     1921 tggatctccc cgatgttggg ggcctggctc cagccaagga cggctacgtt gacggacgtg
     1981 ggtcggtcgg cacgcgcgta gttggcaaga agcatctggt aggcggcctc gagcgggtcg
     2041 ggccagtgcg gcgcgtagcg gctgagcgca tccatcgctg gcgtggcggc gaagagccgg
     2101 ctcagggcgt gcacctcgta cgtgccgcct ggggcagggt tgaactcgat catccggagc
     2161 tgatcatcgc gaccgagaaa accgtcgagg cgacccacgg cgggaggagc gtcggcgggc
     2221 tccaccccga tgagctgatc ctgccaagtc gcaacgccga gtttgcgtcg cagctcggcg
     2281 ctcccgcgag cggcgcggta caggctctcc aaagcggcta cgaccgccac tgctgattgc
     2341 tgggcggccg cgtgttgcgc agcgctgatg aaacaaggcc ttagaatccg gtcgattccg
     2401 tgtccagccg acgtcagccc gggatcagcg cgggccgtag cgacgatggt gcgcgtgcgg
     2461 tcgagcgcgg cttccgagag ggcacgatga tattcgtctc gagccagccg aattgatgaa
     2521 gacgacacgc gccgatgata acgtgaattg ggccactcgg gtgacgatgg ccgagccacg
     2581 cgcccgcaga ctccctcatg acagccaact cccgcgcatc caaaactagc ggatttcaca
     2641 tcgagctctg cggggccgaa cgcgagcgcg ccgccattcg tgcattccgc gaagacatct
     2701 gccgcacgat tcgcctggag gtgggaggcc ccgcggcaga accgctagac tacggaagct
     2761 acctgttcct ttttgccgag ggagagacgc aacccgcggg gatggtggag ttcttcttct
     2821 acgatcaagc gttcgcggcg ttctcggatg ccgtctattc gcaagcggca gatctcggca
     2881 cttacggatc gctcgccgat ttggtccatt tgcgtagcgt cattctcgag gtcggcatcc
     2941 ggcacacgcg tgtgttcgtg tacctcatgg cggccacggt gctcgtgacc cagcggctgg
     3001 gagcaaccca tctgaccgcg ggaacgggga ttcacaacga cgctattctt gctttgcacc
     3061 gtcgcgcggg gatgacacca ctcggtcgat acgtggtcga cggtgcgccg cagcagcttt
     3121 cgcttttggc gcttgcccca ctgcttcagc gcgcgaaagg gctgctcggg cgcggcggca
     3181 ttaccgtaaa tgacagcgcc gctttcgag
//
LOCUS       DASTKU010000568         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1001814, whole genome shotgun sequence.
ACCESSION   DASTKU010000568 DASTKU010000000
VERSION     DASTKU010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001814"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1711
                     /locus_tag="VFQ61_19710"
     CDS             <1..1711
                     /locus_tag="VFQ61_19710"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HET9956736.1"
                     /translation="SGMVTPFFLWKDGSERADQLWQEQDFEIFGRDGSLQTQLMTPGS
                     NDQHRTEHVVNHWLPQAAFERYYTYRMEWTPTRLAFYIDGNLMREETDPNEYWKFLDQ
                     SKSEAAQLRISLWAGDYAWSGAFDSSAAPGAVFVNWVQTYSYTPGKGPNQSDFSPLWR
                     DDFNSLDSNRWWTANWTFEFAINDYVPQNAAAKSGYLVLAFTDENKTGVFPSPPADDG
                     TVLKFEANSGGGSSTPPTPGGPFTLPARIEAEAYQAYYDTSLGNSGNAPCVSGDVDAE
                     GTSDPNGGVCNIAWTDAGEWLEYDVNVAQASTYELIVRAASEEVGQTVHVEVDGVDVS
                     GPLTSPAQGWQAFADVRVPQVALSAGLHTVRLVFDTGMVNVNYLEFLSAQANTVTPPD
                     PGSSGSGSTGGTSSSGGTGSTNTSSGGTHAGTGGALNGGAGGAPGSSGANTCTPTRTT
                     YEAESLPATTGGGSPGGWNLWSNGSLAASHRFGGASSVIRVNAYGQAAVGVAPHMIVS
                     VGGQTIGDVSVQATSYSPYEFFYQPGAGTREVKVSFDNDYYQNGADRNLYVDSIVIDE
                     CPN"
     gene            complement(1786..2499)
                     /locus_tag="VFQ61_19715"
     CDS             complement(1786..2499)
                     /locus_tag="VFQ61_19715"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET9956737.1"
                     /translation="MNSFGVVFLAFATSAVTAVGTVYVIEQKHLFRPVEQKVEQAAVP
                     ELKGLSEADARANLHAVGLVYLAAPREVSSAVPPGRVTRQSVPAGQQVPKQHPVTVVL
                     ADELPKVPEVLRLTQAEATSKLQQAGYKVEVGPSVADAVIPEGAVVTQAPAAGSALAK
                     DQSVQLTLSSGSEPVEVPKIVGLNVTKAQTDLEKLGFKVNVRWVSVAETATYIVLSQD
                     PPAKTKAKPGTVIQLSANR"
     gene            complement(2885..>3079)
                     /locus_tag="VFQ61_19720"
     CDS             complement(2885..>3079)
                     /locus_tag="VFQ61_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956738.1"
                     /translation="NCFSSEPVFAAIPHRSRNTFLFRIAFQFASPFGIQALASRAFSR
                     LRHQTSLGRPTPMAWLDRPT"
BASE COUNT          604 a         1008 c          909 g          558 t
ORIGIN      
        1 gtcgggcatg gtcactcctt tcttcctttg gaaagatggc tcggagcgcg ctgaccaact
       61 ctggcaagag caggacttcg agatcttcgg ccgcgacggt tccttgcaaa cccagctcat
      121 gacccccggt tccaacgacc aacaccgcac cgagcacgtg gtcaaccact ggctcccgca
      181 ggctgcgttc gagcgatact acacgtatcg catggagtgg acccccaccc gcctagcctt
      241 ctacatcgac ggcaacctca tgcgcgagga gaccgatccc aacgaatatt ggaagttcct
      301 cgatcaatcc aagtcagaag cggcgcaact gcgcatcagc ctttgggcgg gcgattatgc
      361 gtggtcgggc gcatttgact ccagcgcagc gccgggcgcc gtgttcgtca actgggtaca
      421 aacttactcc tatacaccgg gtaaaggccc caaccagagc gacttctcac cgttgtggcg
      481 ggatgacttc aactcactcg attcgaatcg gtggtggacg gccaattgga ccttcgaatt
      541 cgccatcaac gactatgtcc cgcagaacgc ggccgccaag tccggttacc tggtgttggc
      601 tttcacggac gagaacaaga ctggcgtttt cccgagtcca cccgctgacg acggcaccgt
      661 cctcaagttc gaggccaact caggcggcgg ttcctccact ccgcctaccc ctggagggcc
      721 tttcacttta cctgcgcgca tcgaggcaga agcgtaccag gcctattacg atacctctct
      781 tggtaactcc ggaaatgccc cttgtgtcag cggtgacgta gacgccgaag ggacttcgga
      841 tcccaacggc ggagtctgca atatcgcctg gaccgacgcg ggcgagtggc tcgaatacga
      901 cgtgaacgtc gcccaggctt cgacctacga attgatcgtt cgggcggcgt ccgaagaagt
      961 agggcaaacc gtgcacgtcg aggtggacgg tgtagacgtg agcggcccat tgacttcgcc
     1021 agcgcaaggc tggcaagcct tcgcggatgt gcgcgtcccg caagtggcac tcagcgcggg
     1081 cctccatacc gtgcgcttgg tgttcgacac gggcatggtc aacgtgaatt atctcgagtt
     1141 cttgtcggcc caggccaaca ccgtaactcc cccagaccca ggctcctccg gctctggttc
     1201 gacgggcggc acgagttcga gcggcggaac cggctccacc aacacgagca gcggcggcac
     1261 ccatgcgggc accggcggcg cactgaatgg cggagctggt ggcgcccctg ggagcagcgg
     1321 agcgaataca tgtacgccca ctcgaaccac ttacgaagcc gaatcgctgc ccgcaacgac
     1381 gggcggcggg agccccggcg gctggaacct gtggagcaat ggcagcctcg ccgcgagcca
     1441 ccgcttcggc ggcgcatcta gcgtgatccg cgtgaatgcg tatgggcaag ccgcggtcgg
     1501 agtggctccg cacatgatcg tctccgtagg gggacagacg atcggagacg tgagcgtgca
     1561 agccacctcg tattcaccgt acgagttctt ctaccaaccc ggcgctggca cccgcgaagt
     1621 caaagtcagc ttcgacaacg actactacca gaacggcgcc gaccgaaacc tctacgtgga
     1681 ttcgatcgtg atcgacgaat gccccaattg aggtagtggt cggaatcgag ccagatagcg
     1741 cgcccaagct ccatgcgcgt tatctggctc tggtcaacca acgattcaac ggttggccga
     1801 gagctgaatc accgtgcccg gcttggcttt ggtcttcgcc ggggggtctt gcgataacac
     1861 gatgtacgtt gccgtctccg cgacgctgac ccaacgtacg ttcaccttga agcccagctt
     1921 ctcgagatcg gtttgcgctt tggtgacgtt caggccgacg atcttcggca cctccaccgg
     1981 ctcgctgccg ctcgagagcg tgagttgcac actttgatct ttggctaacg cggagcccgc
     2041 cgcgggagct tgcgtcacca ccgccccctc cggaatcact gcgtcggcca cactcggccc
     2101 gacttcgacc ttgtagcccg cttgttgcag cttgctcgtg gcttccgcct gcgtgagccg
     2161 caacacctcg gggaccttgg ggagctcatc ggccagcacc accgtcaccg ggtgttgctt
     2221 cggcacctgc tgccctgcag gtaccgactg ccgcgtcact cggcctggag gtaccgccga
     2281 gctcacctcg cgcggcgccg ccaagtacac gaggcccacg gcatgcaagt tcgcgcgtgc
     2341 gtccgcctcg ctcaaaccct tcaactcggg gaccgcggct tgctcgacct tctgctccac
     2401 cggtcgaaac aagtgcttct gttcaatgac gtacacggta cccaccgccg tgaccgccga
     2461 agtggcaaag gcaaggaata cgacaccaaa agagttcatc ttaggctacc ctcgccccat
     2521 cctatccgga atgcgaggcg agaagaagct cacgtagcaa ttccggcgct cgtcaggcct
     2581 gggccgctct cgaacgagcg cgagcctgcg agggagctcc tgaagagcca ccgcctacaa
     2641 ccttgtgtca cgtccccgag ccgcgccggc ccccttaata acgcaacgtc aaccacgacc
     2701 tcgtcccgtc gcacggcgcg cgatcgctcg ttgcagcccg ttgggtcgca aagctgccat
     2761 gtcgtgtgga gacagcattc gcggcccacg gcgcggcgtt acggattgcg gcttgcgcgg
     2821 tatagcgacg aacccggaaa ttcacgacgg tgctcgagag caccgcgtcg cacggcgcgc
     2881 ccgttcacgt gggccgatcc agccatgcca ttggagtcgg acgccccaag ctagtctgat
     2941 gcctcagcct cgaaaacgcg cgtgaagcga gtgcctgaat cccgaagggt gatgcgaact
     3001 gaaacgcgat gcgaaacaga aacgtgttgc ggctgcgatg cggaatggct gcgaacactg
     3061 gttcggatga gaaacagtt
//
LOCUS       DASTKU010000569         9262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1672991, whole genome shotgun sequence.
ACCESSION   DASTKU010000569 DASTKU010000000
VERSION     DASTKU010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9262
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672991"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFQ61_19725"
     CDS             complement(<1..309)
                     /locus_tag="VFQ61_19725"
                     /inference="COORDINATES: protein motif:HMM:NF024800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9956739.1"
                     /translation="MLSGICRCSECGGPLTVSNHRLGACTVKVYVCSYRRSRGDEVCT
                     NTARRPVEEVNSVLVDWIQRNILTEQLVAEVVRRVQARLDEQADNSSSAVPGLEAQAR
                     Q"
     gene            complement(336..797)
                     /locus_tag="VFQ61_19730"
     CDS             complement(336..797)
                     /locus_tag="VFQ61_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET9956740.1"
                     /translation="MIELPDDFRDLLIALSDAGAEFVVLGGHAVAFHGHPRATKDLGV
                     LVRADPNNAKRVYSALAAFGAPLSAFEVGEGDFATYDGVLQMGMPPVRIDILNRADGI
                     TFDEAIAEGAKFELDGRAIPVIGRAALLKNKKAAAREQDIADVKALERARK"
     gene            complement(1496..3376)
                     /locus_tag="VFQ61_19735"
     CDS             complement(1496..3376)
                     /locus_tag="VFQ61_19735"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9956741.1"
                     /translation="MKALLSFVVIGVLSAACARFPDKALSAGPTQTQRGPSAVTSPPR
                     PEGAACGESLGPSPFKNPNSAHVLVADFFESRKEEPEFGDDVTQRLDLELKRFRDEKP
                     GGKVPVAVSNDTVEFARLRCFMTSHEHAEVVAQAWGADLVLWGKAYCDTRLDVQQGIN
                     VPPAASRGRVTATEQGKANVNRVEARDKQPYTVCPSATLPPPETSSRRTDTSKHSLAS
                     LDHRLNLPALSSSAPSQLVYFTIGLSFWERNQPAIAALFFEKSISHVLSSKEENLAPL
                     HMYLGRALLELPGGARHAIAFSQEALREFSGTGTPTEAVLLINIGSALQTEEKSSEAE
                     PFFRRALAVYEKALGKDHPNTARALNYVGSTLLEQGKASEAEGFFRRALAIHEKASGK
                     DRPDAGPVLNNIGTALQSQGKASEAEAFFRRAVAVYERNSMEDHPNAAATLSNMGFSL
                     LAQGKASEAEGFFRRALAVYEKNLGKDHLEAATVLNNIGSTLQEQGKVSEAEGFFRRA
                     LAVYEKNLGKDHLEAATVLNNIGFALQAQGKAPEAEGFFRRVLAIYEKALGKDHPQTA
                     AALTNLGTALQAQGKTSEAEGFFRRALPVYEKALGQDHPLTRRLARSLELLHQSSANR
                     AR"
     gene            3902..4138
                     /locus_tag="VFQ61_19740"
     CDS             3902..4138
                     /locus_tag="VFQ61_19740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956742.1"
                     /translation="MTDQAAIANVADKTVTLSGKTYRIQALADDNYTVLLSGVPVGRI
                     VYSFGAANGVPEGDSITEEELTVIGEAWFAALEA"
     gene            complement(4258..5379)
                     /locus_tag="VFQ61_19745"
     CDS             complement(4258..5379)
                     /locus_tag="VFQ61_19745"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9956743.1"
                     /translation="MNGLPLTNRDHNPPSFSRPAMWPALPWAALLWAALLWAALLASL
                     PGCQSQSKDTQEPTGQTTACSDSSVEGLMACVDPQRYHQDLEHVTGSRPPGSPHWQAV
                     QDLCAERLGALGFTVERQAYEPGVNVIGVLPGSDLASELVLVAAHYDHLDGCPGADDN
                     ATGVAGTLEIARVLGQRRYRRSAVAICLDQEERASNGSLGPTLGSRAFTDQARLRGEI
                     ITAAFIFDMIGYSRSEPNSQRMPPGLEQAFPGPLQQIADNQHRGDFIMLVADPLSASA
                     VDRFEAHAQALELKAVSLQLHPEQTTAPGLSVLRRSDHGPFWEAGYPAVLLTDTAELR
                     NPHYHCENGPDTIEDLDDAFSVRILGATLGAMVDVLEPE"
     gene            5405..6130
                     /locus_tag="VFQ61_19750"
     CDS             5405..6130
                     /locus_tag="VFQ61_19750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9956744.1"
                     /translation="MRRSCVGPEPPAACYRDALGTRILLIEDDRELGRQIADRLRREG
                     YEVEWWTEGRSIRADELAGVRLVILDLMLPGTYGMDILRELRPSAETPCLILSARHDT
                     LDKVRALRLGADDYMTKPFWPDELVERIRARLRRPALSRSEVVRLGALRLDLEAHEVR
                     VADEPVELTPAEFGILAALARKPGVVRTRAWLTERALGPDSEVTTRALDAHVSRLRKK
                     LGASVRIETVWGIGYRLADDGDP"
     gene            6467..7654
                     /locus_tag="VFQ61_19755"
     CDS             6467..7654
                     /locus_tag="VFQ61_19755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9956745.1"
                     /translation="MELAVLDAQFRPALGVALAQSAIGELAPGQALALPTWSDAVDVV
                     VATPWRSSPCAYVAARGHLPDFASSLWPSIGLLLLPVAGISAALLIAIGPVVRRIRRL
                     TAAVTSSASAGFTRKIAIEGSDEIAVLARSFDAASRAVRTQLAAVEQRERILRDFLGD
                     TTHDVMIPLTVLQAHLATLQERERDGGPQDHSVLTAAMDEAHYLGALMQNLGAVAKLE
                     AGQHEPAISTVDLNRLVQRVVSRHRTIAARLQIAIEFAVPDEAIAMAGDITLIEQAVS
                     NVVYNAVRHNRPGGHVAVILEPLPRDGFSLRVLDDGPGIPAEELSLVIARGFRRGRAR
                     TRGVDGSGLGLDITRRVAELHQLELSFKASEAGGLEVILEGLRVRDPAGLTSNHERKP
                     SSE"
     gene            complement(7708..8835)
                     /locus_tag="VFQ61_19760"
     CDS             complement(7708..8835)
                     /locus_tag="VFQ61_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956746.1"
                     /translation="MRSLLRHSREYVVVCASTILALACSSKADPAGPSGCAEGSEQCA
                     CYPNDTCDKGLSCRSNMCVDLGDGAGGKGGLPAAEGGADSDPAGGGKSGEGGRASKGG
                     RASTEPSTEPQAGNEPHEEPGSGGGGSSGQSGSPRGGSPATPQGGQTPQGGGQSSQGG
                     STSSPQGGSTSNPQGGTTSVSQGGSSAGPRDEVSDERILDNFSSCDRFISRVAGRDGG
                     WFTFGDNDVNLTPNTSGSAPLPAASSPPSQFSDHSCAAWVTGGCAETADSCAYAGIGF
                     VLRSDGGGYDLSDFEGLQFDYEGGDLWVVVTGAQGRSFGTGIMASNGSRRTRSFAFSE
                     LVPSGETPKDAVLDLRGVKQIDFTTITPSGFGLAIWDIRLF"
     gene            9008..>9262
                     /locus_tag="VFQ61_19765"
     CDS             9008..>9262
                     /locus_tag="VFQ61_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956747.1"
                     /translation="MPPSPHEQVSVDPELVLLRPGLLFQGRYEVVRCIGSGGMGAVYE
                     VLQRETRRRRALKTLLPSLVSDPEARSRFHLEATVAAEIDS"
BASE COUNT         1621 a         3014 c         2952 g         1675 t
ORIGIN      
        1 ctgccgtgct tgagcctcca agccgggaac ggcggacgag gagttatcgg cctgctcgtc
       61 tagtcgagcc tgtactcggc gcacgacctc ggcgacgagc tgttcggtga gaatgtttcg
      121 ctgaatccaa tcgacgagga cgctgtttac ctcctcgacc ggccggcgcg cggtgttggt
      181 gcacacttcg tcaccgcgcg accgacggta gctgcagaca tagaccttta cagtgcacgc
      241 gccgagacgg tgattgctca cggtcaacgg cccaccacat tccgagcacc gacaaatacc
      301 agacaacacg acggtctccc tacgcgtgct cgggattact ttcgcgctcg ttcgagagcc
      361 ttcacgtccg cgatgtcttg ctcacgcgca gccgccttct tgttctttag caaagcagct
      421 cgtccaatca ccggaatggc gcgtccatcg agctcaaact tcgcaccctc ggctatcgct
      481 tcgtcgaagg tgatcccgtc ggcgcggttc aatatgtcga tgcgtaccgg cggcatgccc
      541 atctggagca cgccgtcgta cgtggcgaag tcaccttcgc cgacctcgaa cgcggagagc
      601 ggcgcgccga acgcggcaag ggcgctgtaa actcgcttag cgttgttggg gtccgcacgc
      661 acgagcacgc cgagatcttt cgttgctcgg ggatggccat gaaacgcaac ggcgtggccg
      721 ccgagcacaa cgaactctgc ccccgcgtcc gacagggcga tgagcagatc tcgaaagtcg
      781 tccggaagtt cgatcatgca gagcgacgcg acacgaacct gcacgggatc tgcgcacgcg
      841 tgtaggtcgg gtgaggcagc ccagcgaggc tgaagctcgc caaagtaagg cggtggacga
      901 gagatagagc ctccgcgccg aagacggggc taaagtcgac ctccggctcg ccgaggcgac
      961 ccttgtgaag ggtcatccgt tcgcgtcgcg attgcgcccg tctctccgcg tcagtcatgg
     1021 cgtcagtgct cgtggcgaag gttatcttcg cacacagatc tagcttcgcc acgcaaagtt
     1081 acagccatgg tccggtgctg cccgtggcgt caacaaaccc atccccccgt ctcggcgcgc
     1141 gtctgccggt ccgttgatga gagagaatcc aggttgtaaa tacgctccat gcgaccaagc
     1201 gcactcgaaa gcacgcgctt tcgccggcca gggggcccag ttacgtttcg aatcccgttt
     1261 gggacgtaga gtgaacgaga cccgttctcg atagctccgt cagcgaaaga agggctcctg
     1321 agccgccagg agctccccga gacggccgtc cctttcaacg gagaagcgac ggccgttttc
     1381 ggtttgtggt gcgttcgcgc ttcggcctcc agccgcttca gctcgaaccg aagcgactcg
     1441 cggcgtccta gcgattacga gaagcttggc cctgctcggg cagccaatgc gtgcgtcatc
     1501 gagcccggtt cgcggaggac tgatgcaaga gctccaggct tctggcgagc cggcgagtga
     1561 ggggatggtc ctggcccaga gccttctcgt aaaccggcag cgcgcgacga aagaagccct
     1621 cggcctccga tgtcttcccc tgcgcttgca gagcggtacc taggttggtc agggcggccg
     1681 cagtttgggg atggtccttg cccaatgcct tctcgtagat ggccaggacg cgacgaaaga
     1741 aaccctccgc ctctggtgcc ttcccctgcg cctgcagtgc aaaaccgatg ttgttcagga
     1801 ctgtcgcggc ttcgagatgg tccttgccca agttcttctc gtacaccgcc agcgcgcgac
     1861 gaaagaaacc ctcggcctct gataccttgc cctgctcttg cagggttgaa ccgatgttgt
     1921 tcaggactgt cgcggcttcg agatggtcct tgcccaagtt tttctcgtac accgccagcg
     1981 cgcgacgaaa gaaaccctcg gcctctgatg ccttgccctg ggcaagcaac gaaaagccca
     2041 tgttgctcag ggttgccgcg gcgttgggat gatcctccat cgagttccgc tcgtacacgg
     2101 ctaccgctcg cctaaagaaa gcttccgcct ccgatgcctt tccttgcgat tgcagtgcag
     2161 taccgatgtt attcaagact ggcccggcgt cgggacggtc cttgccagac gccttctcgt
     2221 gtatggctag cgctcgccta aagaagccct ccgcctccga tgctttcccc tgctccaaca
     2281 gagtagaacc aacgtagttc agggctcttg cggtgtttgg atggtcctta cccagcgcct
     2341 tctcgtagac agccagcgca cgacgaaaga acggttccgc ttccgatgac ttctcttctg
     2401 tctgcagcgc agaaccgata ttaatcagga gaacggcctc cgtaggggtg ccggttccag
     2461 agaattccct cagagcctcc tgcgaaaacg caatcgcgtg ccgcgctccc ccgggcaatt
     2521 cgagtagcgc tcgcccgaga tacatgtgca gtggggcgag gttttcttcc ttggatgaga
     2581 gaacgtgact tatggatttt tcgaagaaga gcgccgctat cgctggttgg ttcctctccc
     2641 aaaaactcag accaatggtg aagtaaacga gctgagacgg tgcactggac gagagcgcgg
     2701 gcaagttcaa ccgatgatcc aagcttgcta aactatgctt gctcgtgtcg gtccgcctcg
     2761 aacttgtctc aggaggcgga agggttgcgc ttggacatac ggtgtagggc tgtttatccc
     2821 gggcttccac ccgattcacg ttggccttac cttgctcagt tgcagtcact cgcccccgtg
     2881 acgcagccgg tgggacgttg atcccttgtt gcacgtcgag ccgtgtatcg cagtatgctt
     2941 tgccccagag cacgaggtca gcgccccagg cctgagccac cacttcagca tgttcgtgcg
     3001 aggtcatgaa gcaccgcaat cgagcgaact cgactgtgtc gttcgaaacc gcgactggca
     3061 ctttcccgcc gggcttttcg tcccgaaatc gcttgagctc gagatctagc ctttgagtga
     3121 cgtcgtcccc gaactctggc tcttccttcc ttgattcaaa gaagtcggcg accaaaacat
     3181 gagcgctatt gggattcttg aacggtgatg gacctagaga ctcgccacaa gccgccccct
     3241 cgggccgcgg cggagatgta acggcgctcg ggccgcgttg cgtctgcgtt ggaccggcac
     3301 tcaacgcctt gtccggaaac cgggcacagg ctgcggaaag gacgccaatc acaacgaacg
     3361 aaagcagtgc cttcacgaca tcaccccagc tgtgttcgag tgatgcgcag ctcagaggcg
     3421 agcattcgcg ggctcatttg cgaaaagatg ggctcggcat tcggaaagat gcgtgctcga
     3481 cacccctcgt ctatacagga gcgcagtcga agaccgcaag acagccggcc cgctcagctt
     3541 acctcaagcc gggcggcgtc aacgtcgttt gcgcactgcc gacgttccct gggcagtcgc
     3601 ctcgaagagg gaatggcaca ggcctattgc ttccgcgcga cccttcttgt ccagtcttcg
     3661 gcagcggagc ccgagaacct gcagcagaat cgctgctcct cgtccggcgc gctgaaccta
     3721 caacgagcgg catgcgcggt gggcgcgacg ggcggtgggt tgacgagtct gcgctaggga
     3781 cggctagtga cgcccggcgc gcccgttgtt ccggggccgc cacccagatc ccggacggca
     3841 atagtgcggg ctgcgttact agtcgtttca atgaggcgca ggacggtgta ggggggcgaa
     3901 catgaccgac caagctgcta tcgccaatgt cgccgacaag accgtcacat tgagcggcaa
     3961 gacttaccgc atccaagctt tggctgacga caattacacg gtgctgctct ccggggttcc
     4021 ggtgggacgc atcgtctatt cgttcggtgc ggcgaatggt gtacccgaag gcgacagcat
     4081 caccgaggag gagctgacgg taattggcga agcatggttc gccgcgctcg aggcttagtc
     4141 accaacgcga acgcgacgga ttgagctcgt ccgggccggt cgcggagagt tgacggctcg
     4201 cgccggcctc tccgccgtgg gctcctccct ctgattcgaa tgcacgccta cgggggctca
     4261 ctcgggctcg agcacgtcga ccatggcccc gagcgtcgcc ccgaggatcc gcaccgagaa
     4321 cgcgtcgtcg agatcctcga tggtgtcggg gccgttctca caatggtagt gcgggttacg
     4381 gagctcggcc gtgtccgtga gtaggaccgc aggatagccc gcttcccaaa aggggccgtg
     4441 gtcggaccgg cgcagcacgc tcagccccgg cgcggtagtc tgctctgggt gtagctgcag
     4501 ggaaaccgcc ttcagctcta gtgcctgtgc gtgggcttcg aagcggtcga ccgccgaggc
     4561 cgagagcggg tcggccacga gcatgatgaa gtctccgcgg tgctggttgt ccgcaatctg
     4621 ttggagtggc cccggaaagg cctgctcgag cccgggcggc atccgctggc tgttcggctc
     4681 gctccgcgag tagccgatca tgtcgaagat gaacgccgcc gtgatgatct cgccgcgcag
     4741 ccgcgcctga tccgtgaagg cccgtgaccc gagggtcggg ccgagcgagc cgttcgaggc
     4801 gcgctcctcc tgatccaagc agatcgcgac cgcactgcgg cgatagcgcc gctggccaag
     4861 gacgcgcgcg atctcgagcg tgccggccac gccggtggcg ttgtcgtccg caccggggca
     4921 accgtccagg tgatcatagt gcgctgcgac gaggaccagc tccgaagcga ggtcgctccc
     4981 ggggagcacg ccgatcacgt tcactccggg ttcgtaggcc tggcgctcga cggtgaagcc
     5041 gagcgcgccg aggcgctcgg cgcagagatc ctggaccgcc tgccaatgcg gggagcccgg
     5101 cgggcgagag ccggtgacgt gctccagatc ttggtgatag cgttgtgggt ccacgcaagc
     5161 catgaggccc tccaccgagg agtcgctgca cgcggtggtc tgtccggttg gctcctgagt
     5221 gtccttgctc tggctctggc atccagggag cgaggcgagc agggccgccc agagcagagc
     5281 cgcccagagc agggccgccc agggcagcgc cggccacatt gcgggccggg agaacgaagg
     5341 gggattgtgg tcgcggttgg tcaggggcaa gccgttcatt gcggcccagc ttgcttgaac
     5401 aatcttgcga agatcttgcg tcggacccga gccgcccgcc gcatgttatc gtgacgcgtt
     5461 gggcacgcgc atcctgctca tcgaagacga ccgcgagctt ggccggcaaa tcgccgatcg
     5521 cctgcggcgc gagggttacg aggtcgagtg gtggaccgaa gggcggtcca tccgcgcgga
     5581 tgagctcgcg ggcgtccggc tggtgatcct cgatctgatg ctgcccggca cctatgggat
     5641 ggacatcctg cgcgagctcc gcccaagcgc cgagacacca tgtttgatct tgagcgcacg
     5701 ccacgacacc ctggacaagg tgcgggcgct ccggctcggc gccgacgact acatgaccaa
     5761 gccgttctgg ccagacgagc tggtagagcg gatccgcgcg cggctgcgcc ggcctgcgct
     5821 gagccggagt gaggtcgtcc ggctcggcgc gctgcgtctg gatctggagg cccacgaggt
     5881 tcgcgtggca gacgagccgg tcgagctgac tcctgccgag ttcggcatcc tggcggcgct
     5941 ggcgcgcaag cccggcgtcg tacgcacccg cgcctggctg acggagcggg cactcggccc
     6001 ggacagcgag gtcacgacac gcgctctcga cgcgcacgtc tctcggttgc ggaagaagct
     6061 cggagcgtcc gtgcggatcg agacggtctg gggcatcggt taccgcctcg cagacgacgg
     6121 agatccatga agctgcgtct ccgcctggcg gtcgcgatca tcgccgcggt cgtgcccacc
     6181 ctgctcgcgc tgacttgggc cgagctgcgc gagcgccagg cggacgccgc agaggcgctg
     6241 atcgccgcaa cccggcagac gctcgcggat cctgagctcg tgccgggctg tgcgatggac
     6301 ccagtgggct gggcccgcgg cgcgcactcg ctgctaaacc cgccgccgcg cgggccgctg
     6361 ccagtgggcg ccgcgcaaga gctggcggcg ccagcgggac gtgcccttcg atccgcgcag
     6421 tgccagcgcc gagaagcctc ccgcgctcgc gattcagctg gctcccgtgg aactcgcggt
     6481 gctcgacgcc cagttccgtc cggcgctcgg cgtggcactc gctcagagcg cgatcggcga
     6541 gcttgcgccc gggcaggcgc tcgcattgcc gacctggagc gacgcggtcg atgtcgtggt
     6601 tgcgacgccg tggcgatcca gcccctgcgc ttacgttgcc gcccgaggcc acttgcccga
     6661 tttcgcgagc tcgctctggc cgtcgatcgg acttctgctt ttgcccgtgg cgggcatctc
     6721 cgcggcactg ctgatcgcga tcggacccgt agtgcggcgg atccgccggc tcacggccgc
     6781 ggtcacctcc tcggcctcgg cagggtttac gcgcaaaatc gcgatcgagg gcagcgatga
     6841 gatcgcggtg ctcgcgcgct cgttcgacgc agccagccgc gcggtgcgga cccagctcgc
     6901 cgccgtagaa cagcgcgagc gcatcctgcg cgacttcctc ggtgacacca cccatgacgt
     6961 gatgattccg ctcaccgtgc tccaggccca cctcgccacc ttgcaggagc gggagcgcga
     7021 cggcgggccc caggatcact cagtcctgac cgccgcgatg gacgaggcac actacctcgg
     7081 cgcgctaatg cagaacctgg gtgccgtggc caagctcgaa gccggccaac acgaacccgc
     7141 aatcagcacc gtagacctca acaggctggt tcaacgcgtc gtgagccgcc accgcacgat
     7201 cgccgctcga ctccagatcg cgatcgagtt cgcggtgccg gacgaggcca tcgcgatggc
     7261 tggcgacatc accctgatcg agcaagccgt gagcaacgtc gtctacaatg ccgtccgcca
     7321 taatcgaccc ggaggccacg tcgccgttat cctcgagcca ctgccccgag acggcttctc
     7381 cctccgcgtg ctcgacgatg gccccggcat ccccgccgaa gagctctcgc tcgtcatcgc
     7441 ccgcggattc cgccgcggtc gtgcacggac acgcggcgtc gacgggtcgg gcctcggcct
     7501 cgacatcacc cgaagagtcg cggaactgca ccaactcgag ctcagcttca aagcgtcgga
     7561 ggcgggcggc ctcgaggtta tcttagaggg cctgcgcgtt cgcgatcctg ccgggctgac
     7621 cagcaatcac gagcgcaagc cctcgtccga gtgaccaacg cgttccacgc ttccgctagg
     7681 tcccgcctcg aatcagctcg acgccgctca gaacaaacgg atgtcccaaa tcgccagacc
     7741 aaagccggac ggggtgatgg tcgtgaagtc gatctgtttg acgccgcgca aatccaggac
     7801 cgcgtccttc ggcgtttcac ccgagggtac gagttccgaa aaggcgaagg aacgagttcg
     7861 gcgtgagccg ttcgaagcca tgatgccggt gccgaaagaa cgtccctggg cgccggtgac
     7921 gacgacccaa aggtcgcccc cctcgtagtc gaattgaagt ccctcgaagt cgctgaggtc
     7981 gtaaccgccg ccatcgctac gtaggacgaa gccaatgcca gcataggcgc aagagtccgc
     8041 agtttccgcg caaccacctg tcacccatgc tgcacaagaa tgatccgaga actgggaagg
     8101 aggagagctg gctgcaggca acggcgcact gccgctggtg tttggagtga gattcacgtc
     8161 gttgtctccg aaggtgaacc agccgccgtc gcgcccagca acgcgactga tgaacctgtc
     8221 gcaactcgag aagttgtcga ggatgcgttc gtccgagact tcgtcgcggg ggcctgcgga
     8281 gctgccgcct tgggacaccg aggtcgtgcc accctgcgga ttcgacgtag aaccgccttg
     8341 cgggctcgac gtggatcccc cttgcgagct ctgcccgcca ccttgcggcg tttgcccgcc
     8401 ttgaggagtg gccgggctgc caccgcgcgg gcttcctgac tggcctgaac tccctcctcc
     8461 acctgatccg ggctcttcgt ggggctcatt gcctgcttgc ggttcggtgc tgggctcggt
     8521 gctggctcgg cctcctttgc tggcccgccc accctcaccg ctctttccgc cccctgcagg
     8581 gtccgaatcg gcgccacctt ccgcggcagg aagaccgcct ttgccgccag cgccgtcgcc
     8641 caaatctacg cacatgttgg atcggcaact caggcctttg tcacaggtat cgttcggata
     8701 gcaggcgcac tgttcgctgc cctcggcaca accactcgga ccggcgggat ccgccttcga
     8761 gctgcaggcc agtgccagta tggtcgatgc acaaaccact acgtattcgc gcgaatggcg
     8821 aagcaaagat cgcatttggg tgactccgtt cggtctcggc gggatgaggc cgcgactcgt
     8881 gccagttaaa gacgtgagac tccggcgaga cgtcacgccg gactcgagaa aatcccgttc
     8941 taaagtcggt cgaccaacct gaggggtacg tcgaccggca caaagtgatg gtaaggtcgc
     9001 ggttcgcgtg ccgccttccc cacacgaaca ggtctctgtc gatcccgaac tggtgctgct
     9061 ccggcccggt ctgctgtttc agggccgcta tgaagtcgtg cgctgtattg gtagcggagg
     9121 catgggggcc gtgtacgagg tgctgcagcg tgagacgcgc cggcgtcgcg cgctcaagac
     9181 cctattgccc agcttggtat ccgatccaga ggcccgcagc cggtttcacc tcgaagcaac
     9241 ggtcgcagcc gagatcgaca gt
//
LOCUS       DASTKU010000570         3454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1450736, whole genome shotgun sequence.
ACCESSION   DASTKU010000570 DASTKU010000000
VERSION     DASTKU010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450736"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..356)
                     /locus_tag="VFQ61_19770"
     CDS             complement(90..356)
                     /locus_tag="VFQ61_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956748.1"
                     /translation="MRPPPLVSTDRLESSEPSVRGSEARGIRGYECDVCGRRFEGEPA
                     SSGLFIWSRGDERRYEEPPLCEECADRVTLGALYTFDFDDEDEG"
     gene            complement(562..2766)
                     /locus_tag="VFQ61_19775"
     CDS             complement(562..2766)
                     /locus_tag="VFQ61_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET9956749.1"
                     /translation="MNPVMMAVLLLGFWGLFAFSAQRRLALLRTGAPTHESRTDRLPD
                     RLRMVLNQVLMHRKMRYYWLAGSAHYLIFTGFAVLLLRTLMLWGRGFDPAFNLWLLGP
                     EPVFGVPLGSIYSFLKDVFALLVLCGAGVFLYLRLIRRERRMTLSGEALVILGIICTM
                     MLSDLIYDGASLVLSTRYAAECAAASKDYCASAAELIAPLGAIHEIGFRAAEPGGSMA
                     AVLLSGLPSAALTVLAQIGFWTHSTLVLVFLNLLPYTKHFHIITGVPNVFLGDLTPRG
                     RLRPIAPSTEALMATVEKATESEDLLEARVGYARLKHFTWKDMLDLYTCTECGRCSDN
                     CPATLTGKLLSPKHFTLALRDQLYGHAPSGNTQEASAGESAAATSEQPSGNTQAEVAE
                     REKLNLVPGVIAPEVLWACTTCRACEEQCPVSITYVDKIVQMRRNLVTIKGEFPQELA
                     KPFEGMEVNGNPWNLSRMDRPAWAEGLEIPTLAEKPATPVLFWVGCAASYDDRARRIA
                     RSTASLLKAAQVDFAILGEEENCTGDPARRAGNEYVFSMLAEANAQTLNNYQAQGGVK
                     RIVTTCPHCFNTLKNEYPDFGAKFEVVHHTDFLLGLLAERKLTPKNAVKARVAYHDSC
                     YLGRYNDVYESPREILRSIDGVELVEVEYWNRNKGLCCGAGGAQMWMEEQNKDRVNVK
                     RTLQLIQTGADTVATACPFCMTMVRDGIKSQTAEEKVRNMDVVELLAEACGV"
     gene            complement(2972..>3454)
                     /locus_tag="VFQ61_19780"
     CDS             complement(2972..>3454)
                     /locus_tag="VFQ61_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956750.1"
                     /translation="PARSSEELAAGSLVVVRVPDRGGAAPANSPLPRGMLFLKITPAA
                     ASDASVHRVTDSGSQLRLESEIVPPSSAEFASPDSRGPESSASECGAASLELVAGSRG
                     AALESVQPEAIEQDDADVQRVLNTRSQSSGARRRFGALIWLIWGAGVLAVSYYFLMHR
                     "
BASE COUNT          665 a         1169 c         1025 g          595 t
ORIGIN      
        1 ggcgtcgacg acgaagtcga tgcgctcgat acatgacgcc atcactcgac gcatcggctc
       61 ccacccgagg cgcagccaaa gctgcgagct cagccctcgt cttcgtcgtc gaagtcgaag
      121 gtgtagagcg cgcccagcgt cactcgatcg gcgcactctt cacagagcgg cggctcttcg
      181 taacgacgct cgtcgccccg ggaccagatg aacaggcctg aactggcggg ctccccctcg
      241 aatcgacgcc cgcaaacatc gcactcgtag cctcggatcc cgcgagcttc cgaaccgcgc
      301 acggaaggct cgctggactc cagtcggtcg gtcgagacga gcggcggtgg cctcatcgac
      361 gtggagtata gcgacctaca gcccactgcg tccaccgaca atgcaaaatc gcggacgcga
      421 gcacacggcc tcgagatggg ggaatcgagc aaccgctgcg agctgatccc cccgaggatg
      481 acttgcctca ggatgagcca gcgatcccct tgcggaagct cccgtgtccc gctcgcacct
      541 ctgtcccgcc ccctacactc gctacactcc acaagcttcc gccagcagct ccacgacgtc
      601 catgttgcga accttttctt cggccgtctg actcttgatg ccgtcgcgca ccatcgtcat
      661 gcagaacgga caagccgtcg cgaccgtgtc cgcaccagtt tggatgagct ggagcgttcg
      721 cttgacgttc acgcgatcct tattctgctc ctccatccac atctgagcgc cgcccgcgcc
      781 gcagcaaagc cccttgttgc gattccagta ctcgacctcg acgagctcga caccgtcgat
      841 gctgcgtaag atctcgcgcg gagattcgta aacgtcgttg taacgcccca gataacaaga
      901 gtcgtgatac gcgacccgcg ccttgaccgc gttcttgggc gtcagcttgc gttccgcgag
      961 caagcccagg aggaaatcgg tgtggtgaac gacctcgaat ttggctccga aatcggggta
     1021 ctcgttcttc agagtattga agcagtgcgg gcaggtggtc acgatgcgct tcacgccccc
     1081 ttgcgcctgg tagttgttca acgtctgcgc gttggcctcc gccagcatac tgaacacgta
     1141 ctcgttgccc gcgcggcgcg ccggatctcc cgtacaattc tcctcttcgc ccaagatcgc
     1201 gaagtccacc tgggccgctt tcagcagtga ggcggtggag cgcgcgatgc gccgtgcccg
     1261 gtcgtcgtag ctcgccgcgc agcccaccca aaacagcacg ggggtcgcgg gcttctcggc
     1321 gagcgttggg atctcgagcc cttccgccca cgccgggcga tccatgcgcg acaaattcca
     1381 gggattgccg ttcacctcca tgccctcgaa cggctttgcc agctcttgcg ggaactcacc
     1441 cttgatggtg accaagttcc gccgcatctg aacgatcttg tctacgtacg tgatgctgac
     1501 cgggcactgc tcttcacacg cgcgacacgt ggtacacgcc cagagtactt ccggtgcgat
     1561 cactcccggg accagattca acttctcgcg ttcggcgacc tccgcttgcg tgttgccgct
     1621 cggctgctcg ctcgttgcag ccgcagactc accagcgctc gcttcctgcg tgttgcccga
     1681 cggcgcgtga ccatacagct ggtcccgcag cgcgagcgtg aagtgcttcg ggctgagcag
     1741 cttgcccgtc aaggtcgccg ggcaattgtc ggagcagcgc ccgcactcgg tgcaggtgta
     1801 cagatccaac atgtccttcc aggtgaagtg cttgagccgc gcgtaaccta ctctcgcttc
     1861 gagtaagtcc tcgctctcgg ttgctttctc caccgtggcc atcagcgcct cggtgctagg
     1921 cgcgatcgga cgcaagcgcc cccgcggcgt caaatcgccg aggaatacgt tgggcacccc
     1981 ggtgatgata tggaagtgct tggtgtaagg cagcaggttc aagaacacca agaccagagt
     2041 cgagtgcgtc cagaatccga tttgcgccag caccgtgagg gccgcggagg gcaaaccgct
     2101 cagcaacacc gcggccatcg agccaccggg ttcggcggcg cgaaaaccaa tctcgtggat
     2161 ggcacccaga ggcgcgatca gctctgccgc cgaagcacaa tagtccttgc tggccgccgc
     2221 acactccgcg gcgtagcgcg tcgacaatac gaggctcgcg ccgtcgtaaa tgaggtcgga
     2281 cagcatcatg gtgcagatga tgcccagaat cactagcgcc tccccgctca acgtcatgcg
     2341 acgctcgcga cggatcagac gcaagtacag aaacacgcct gctccgcaca acacgagcag
     2401 cgcgaacaca tccttcagga agctgtagat gctgccgagg ggcacgccga agaccggctc
     2461 cgggccgagc aaccacaggt tgaatgccgg gtcgaagccg cggccccaca gcatcagcgt
     2521 gcgcagcagc aacacggcaa acccagtgaa gatcagatag tgggcgctgc ccgcgagcca
     2581 gtagtagcgc atcttgcggt gcatcagcac ttggttcagc accatgcgca ggcgatccgg
     2641 aaggcgatcc gtacgcgatt cgtgcgtggg cgcgcccgtt cggagcaggg ccaaacggcg
     2701 ttgcgcgctg aacgcgaaca gcccccaaaa cccgagcagc aacacggcca tcatgaccgg
     2761 attcatggcg cgaacctcat tgcgcgaaac ataaccccgt tttgtcgccg agctgtcgtc
     2821 gaatttgcgg caggccatgc ggagcgccct ttcgagctcg gctggcctga tttttggact
     2881 accgcgctcg agacccttgg ccgccgctcg tgcgcatcag ctcagaaacg agcgacgcgg
     2941 agctcagcga gggcctttcg actgcgacga ctcagcggtg catcaagaag tagtaactga
     3001 cggcgagcac cccagcgccc cagatcaacc agatcaatgc gccgaaacgc ctgcgcgctc
     3061 cgctcgactg tgaccgcgtg ttcaaaacgc gctgcacgtc cgcgtcatct tgctcgatcg
     3121 cctcaggctg aaccgattcc agcgccgcgc cgcggctgcc ggcgaccagc tcgagcgacg
     3181 cagctccaca ctcagaggcg gacgactcgg gaccccgaga gtcggggctc gcgaactccg
     3241 cagacgacgg cggcacgatc tcactctcga gcctcagctg gctgcccgaa tcggtcacac
     3301 gatgcacaga tgcatccgag gcggcggcag gagtgatctt caggaacaac atgccgcgag
     3361 gtaacggcga attggcgggt gcggcgccgc cgcggtcggg aacgcgcacg acgactaggc
     3421 tgcccgcggc gagttcctca ctcgagcgag cggg
//
LOCUS       DASTKU010000571        12880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1002329, whole genome shotgun sequence.
ACCESSION   DASTKU010000571 DASTKU010000000
VERSION     DASTKU010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12880
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002329"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFQ61_19785"
     CDS             <1..278
                     /locus_tag="VFQ61_19785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956751.1"
                     /translation="LECIRSTLVEEERELLVLRVDRNLAWEDIAIIMSTRAVPVSANG
                     LVQRFSRLKRKLKRLREELPETCGRVDCANSEACARRRSLQVEVRST"
     gene            275..1885
                     /locus_tag="VFQ61_19790"
     CDS             275..1885
                     /locus_tag="VFQ61_19790"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9956752.1"
                     /translation="MSSSLATDSSFLLELARSPRIRPPLRFAPGELLDGRYELDSQLG
                     EGSFGVVYKARDHKLGRTVAVKVIRREGEGDANAVRELLAAQIEPAPPNIVTLFDAGV
                     HDGYPFLVFEYLEGQTLQDRIRLGKMSAREALFIACQVARGLRAAHRAGVLHRDLKPS
                     NIFLTHAGEVKLLDFGLAHRLEDRQLLIPRGGTPGFAPPEQASGQPGEARSDVFSAAM
                     TLYVCLYGGLPLNSLTFDFNRFRTEPPPPEAIHRLVAPRALKRLLVRATERETHKRPD
                     SSEWHDRLERLLRQETAPVPRVASIALGLSMTAVGVVAALSWLPSWRVGAPFSAKSTA
                     TPAESSQALQANVTATAASPKTTSGEVTKLNTALPSAPRAAALSVVGPASVETTPALK
                     SLSGVQSAALSQPRASLRGSRAMPPALGAEMPTFAETTSLALLGVAKRGANERGASSL
                     APASPKRVDSESKSEAHAVQRARAAPAAPTAQAAQAKAKSTESPDAKRILERRRRASA
                     EREWDSQRERAIARDRRQPGSDPTIPFM"
     gene            complement(1928..2851)
                     /locus_tag="VFQ61_19795"
     CDS             complement(1928..2851)
                     /locus_tag="VFQ61_19795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956753.1"
                     /translation="MRTFRGLCGSRLSLAAGLFGFLLSHPVWAGKLDRAGDAARGRSD
                     SSSSSSSSASSSASSDSEGDYSDSSSCCSSSGGQAFGVMFYVLASPWTFPHAIVESGR
                     SENGEQRTRFADYPYAPGSTGYLLEQPEPTVFGAAPVRAPDAQWAAAQLAVESGIGIH
                     DGILRNGVRARLQLPYRLELDADWSVYREADAGVADQAWIGREHLTVRFAEARAVQFR
                     SGLGPQHYCDGEGCVHGVDLTWGFDAFPGRPVVLSAEGAVGNLGQAFAPSLRARLGFM
                     LGPVEIGAGWQQRWIAGIALGGPHVSVGAWF"
     gene            complement(3037..3711)
                     /locus_tag="VFQ61_19800"
     CDS             complement(3037..3711)
                     /locus_tag="VFQ61_19800"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9956754.1"
                     /translation="MQKPSRKGLFKYLLALALAAAAGAEFSDSRLLAIPETLVSEVAD
                     EVAFEPSEWPREARSRVLWLLSTDPRPRVRARVARNLGALAKIAPSRSLSLIERLARD
                     ESVRVRVAAAEGLERALAVMHPGQRIELLCQWATANQPNQREVLARALLNHVHTPVSD
                     FVIDTLATDERPHTRLHAARAAAQHYSRSPAQYAETLSRLARDADARVARAARVGLND
                     GRIERA"
     gene            3868..4623
                     /locus_tag="VFQ61_19805"
     CDS             3868..4623
                     /locus_tag="VFQ61_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956755.1"
                     /translation="MNTSSQLEANAFPIEPATRATPERHVVCTEALSFLAAHPAESGS
                     SVITSLPDVSELPELGFEGWRAWFLRAARSVLAWTPEDGVSIFFQSDIRYRGAWVDKG
                     YLVQRAAEDLGQELVFHKIVCRKTPGTITHGRASYSHLLCFSKRSRGMPRAPGPDVLA
                     DAGAMSWSKAMGENACRLACRYLASETATRLVVDPFCGQGTVLAVANEFGFDALGVDL
                     SQRKCRVARRLQVGVPKPAPELDEPRQLEEPPT"
     gene            4761..5525
                     /locus_tag="VFQ61_19810"
     CDS             4761..5525
                     /locus_tag="VFQ61_19810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9956756.1"
                     /translation="MPPLAQDLRAAANSVIFPTWTALCVSAGICAGAVTRDSDLFYRW
                     QRHWARGPFVLCGIPLHVSGQQNMEPDRGYVVIANHSSYMDIPAVFASLPTLPSFFAK
                     RELSRIPFLGQALRSGRHILVDRGSRSSARDTLERAAAHVRRGGSVLIFPEGTRQTRD
                     AIGTFKSGAFRLAKLAQAPILPVGIRGTRHVLPKHGRLLRSHPVQLQIGAPISRTEVL
                     DIDLGSLTRLCRAKVAELAGLPIEEATGESGETATG"
     gene            5530..8868
                     /locus_tag="VFQ61_19815"
     CDS             5530..8868
                     /locus_tag="VFQ61_19815"
                     /inference="COORDINATES: protein motif:HMM:NF042814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HET9956757.1"
                     /translation="MRPHLLAGPVRELRESLPQALAGGAQPIDPTQSALAHLLRSAAD
                     RVYADLVSRFGPGLLDSFEPPSSDLASSAEWERMLSQRLAADSLGPAWLDDTSLGWIY
                     QFWHEADRIAIDARLDAQHKLEPQEIATKTQVFTERYMIDWLLQNSLGTLWTRVCQRH
                     GWRSQVLARWPYFKACPEPDSTESLALPQRLNELKLLDPACGAGHFLVVAVDYLLSLY
                     REEAVLSGQGGSDREFVESILENNLFGIDLDARAVRVAASSLLLKSLRVASDARPRRL
                     NLVGTEFATELARIQGRDPLYEQLAEALSRAKAPATTVSRVLALLSSAAHLGTLLKVN
                     LELDAWLTHSPHGPEVRRALEQLLTTHTRALGGARALDAGSETGFGLRALRAQEPDGL
                     LFWRLTNERQYHVICGNPPYFGTQSIADASYLDRRYPESRENLCTALLDRAMELVLPG
                     GLLAFVTQRNWLYVRQLQYFRRRMLETFAPFRVADLELGGFESLKTVDGILWVARGGS
                     SGSCWVTRAPAPEPTSKAAALREPRETYVTHPSLLARLPGSPFVYRWPREFAERYLSE
                     PLLGERYPVRVGMKTSDNLRFVRYPWELPAAVTRRAAEQPAGADWVPYIKGAEGKTWI
                     EPLSHLVCWREHGLEIRIALEAAYQQKPQAEKYFFKRGVAFNTIGRSFVARAHRYASL
                     FDVAGSSVFPDDLAEAVCLLNSRFAREIVESLNPTINFQVGDVARIPARRDPEARALF
                     ALLESAFETFERSDETSFRFQRPAATTWIGAQAYAARAVNRPEGAALPEYEPDARPPT
                     PEAAFSYAFGVAIGRFDAQGKGWLDTAPSSALPHGLLFIGQLGHDSLQHPAARGVLET
                     FAGLRASPTAPQSGTAPSSERELSGLRKFLRREFFTSHRKQYRNRPIYLPLASSQANF
                     VAFVSVHGFNPRTLRALVDEYLTPELACIEAALVASEPPHAASEQPRASAASSRSGAE
                     LAKLRSELVCWIRDIGTVASVGPWSPDALTPPREVDAEFLWEMDDGVLVNSAALWPLL
                     APLWSEPKKWWSQLARRSGPKGCHFDWSRTARRYFPQRVERACAADPVVASAHGELER
                     YHPQLATRWRERLQELGDQTGNPG"
     gene            8969..9940
                     /locus_tag="VFQ61_19820"
     CDS             8969..9940
                     /locus_tag="VFQ61_19820"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HET9956758.1"
                     /translation="MPGTSARLLRLLALLQARKYWPGPELAERLEITTRTLRRDIDRL
                     RELGYPVQAASGVAGGYQLGAGATLPPLLLEDDEALAVALGLLTTASGAVVGLDEAAL
                     RALAKLEQVLPSRLQRRMGSLRASLTSLESHAPKVEASVLGSVAAACRDRDRLQFRYE
                     DARGQSRQRDVEPAGLVHMGGRWYLVAWDLERVAFRTFRLDRIAKKVSLGPRFSPREL
                     PEGGDLRAYVARQVTSVVYPVRAEIEFAAPLATLRERVPPGVGMLEALDRDRCLLKTG
                     AHSMSHLALHIALLQVDFVVRSPPEMIEYLERLRSRLDRALPASSSG"
     gene            complement(9998..10891)
                     /locus_tag="VFQ61_19825"
     CDS             complement(9998..10891)
                     /locus_tag="VFQ61_19825"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HET9956759.1"
                     /translation="MLSRLRSSFTLALVFSGFHLTIGCSSEQAGSSCTTGTERCPCYP
                     NNTCNDGLACFSNRCVVPPSETGGTTSGLGGQTSASGGRSGSDSTGEAGAKEPTTSGG
                     TTNGQAGATGEPELGGTSAGGSSSTNTTAGTTANGGSSSGGASNMTGGMSGNNLVGNG
                     DFAQGEAHWKVESHNGGGISLSTNGQYCLKGQGALSFTIGYPKEGEPGLSLNDGRHYV
                     FSFRARASAATDVDAKVGQMEAPYDAATSKSFSFGNSYQTYSQEFDYDAGLGVLGLAF
                     TGSIGNNQELCLDDVTLAEKP"
     gene            11072..>12880
                     /locus_tag="VFQ61_19830"
     CDS             11072..>12880
                     /locus_tag="VFQ61_19830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9956760.1"
                     /translation="MQEDAGARETPEAVGDVLAAFREIVDTAVTPTWLLSPDSSMLHA
                     NGPLHDWCGASARELQGVGWLTRLHPEDAAHSSAYWQRLFADGQSTEIYFRLRRMDGS
                     YAGLCANCIPLRDGSGAWLVTTRLESPASGTAAVLTRLDPNANGHALRALFFEHMLEP
                     AFIWELSEKRPIIDWNRGAEQTFGFESQEAIGRDARELLQTRACTRWSEIEASLKREG
                     SWQGELLQRHRLGDTLAIETRLQLVLCAGRPPLVLESGIDNSALRQGERAVIEGQARL
                     RLALSSARIGIWEWRLESDRLVWSSEAKTVLGSPPEEFAPTLPALIARVHPDDRASVR
                     ERLEDAVAHVRDVELEFRCPRHDHVRWLRCRGIVERDEGGRATRVVGTISDVTPERLQ
                     EASLRESEQRARARADELSALMDALPAAVWIAHDRHAKQVTGSRVGHEILRMPWGANL
                     SKTAEDTAPVQHFTVLKDGLELQPDELPLQVAALTGKEIRDFEEEIRFGDGSSIYLYG
                     SAVPLLDEKREPRGAIAAFIDVTRIKHAESEQRAAARRKDEFLAILSHELRNPLAPIL
                     TAVHLMKLRGDVPAPNERDVIERQAKHLVRLVNDLLD"
BASE COUNT         2298 a         4276 c         4066 g         2240 t
ORIGIN      
        1 ggctcgaatg catccgctcc accttggtcg aggaggagcg cgagttactc gtgctccgag
       61 tggatcgaaa cctcgcgtgg gaagacatcg caatcatcat gtccacgcgc gcggttccgg
      121 tgagcgcgaa tggtctggtg caacgcttct cccgactgaa gcgcaaactg aagcgcttgc
      181 gcgaagagtt gccggagact tgcgggcggg tggattgcgc gaactcggag gcctgtgcga
      241 ggcgtcgctc attgcaggtc gaggtgaggt ctacatgagc tcgtccttgg caacggactc
      301 ttccttcttg ctggagctgg cgcgctcgcc acggattcgt cccccgctgc gtttcgcgcc
      361 gggggagctc ctggacgggc gttacgagtt ggactcgcag ctcggcgaag gttccttcgg
      421 ggtggtttac aaggcacgtg atcacaagct cggccgaacg gtagccgtga aggtcattcg
      481 ccgagagggt gagggtgatg ccaacgcggt gcgagagcta ctggccgcgc agatcgagcc
      541 cgcgccgccc aacatcgtga cgctgttcga cgccggcgtt cacgacggat atcccttttt
      601 ggtcttcgaa tacctggaag gccagactct ccaggatcga atccgtttgg gaaagatgtc
      661 tgcgcgtgag gcgctgttca ttgcgtgtca ggtggcgcgc gggctgcggg cggcgcatcg
      721 cgcgggcgtg ctgcaccgcg acttgaagcc gagcaacatc ttcttgaccc acgccgggga
      781 agtcaaactg ctcgatttcg gactggcaca tcgcttggaa gatcggcagc tactgatccc
      841 acgcggggga acaccgggct ttgccccacc cgagcaggca tccgggcaac cgggtgaagc
      901 gcgcagtgac gtgttcagcg ccgcgatgac gctctacgtg tgcctatacg gtgggctgcc
      961 attgaacagc ttgaccttcg acttcaaccg ctttcgcacc gagccgccgc cgccagaagc
     1021 gatccatcgc ttggtcgccc cccgcgcgct caagcggctc ctcgtgaggg ccaccgaacg
     1081 agagacgcac aagcgccccg acagctcgga gtggcacgat cgcctggagc ggctgctgcg
     1141 gcaagagacg gcgccggtgc cgcgggttgc ttcgattgcg cttggcttga gcatgacggc
     1201 agtcggcgtg gtcgcggccc tctcctggct gccgagttgg agggtcggcg ctcccttcag
     1261 cgcgaagagc acggctacgc cagccgagag cagccaagcc ttgcaggcga acgtcaccgc
     1321 gactgcggca tcccccaaga ccacgtcggg tgaggtcacg aagctcaaca ccgccctgcc
     1381 ctcggcccca cgcgccgccg cgctctcggt ggttggcccc gcgtcggtcg agacgacccc
     1441 ggctttgaag tcgctgagcg gagtgcagag cgcggcctta tcgcaaccca gagcgagttt
     1501 gagggggtca cgagcgatgc cgcccgcgct cggggccgag atgcccacgt ttgcggagac
     1561 gacctcgttg gcactgctgg gcgtcgccaa acgaggagca aatgaacgtg gagcttcgag
     1621 tttggcgccc gcgagcccca agcgggtgga ttcagagtcg aagtccgagg ctcacgccgt
     1681 acagcgcgct cgggctgctc cggcagctcc gacggcgcaa gccgcccaag ccaaggcgaa
     1741 gtccaccgaa agccctgacg ccaagcgaat cctcgaacga cggcggcggg cgagtgcgga
     1801 gcgggagtgg gactctcagc gcgagcgagc cattgcgcgc gaccgcaggc agccgggttc
     1861 tgatccgaca atcccattca tgtgagccgc gcgtgggtct gcacgccgag cgccggttca
     1921 acctggttca aaaccaggcg cccacactaa cgtggggccc gcccaaagca atgcccgcga
     1981 tccagcgttg ctgccagcca gctccgatct ctaccggacc gagcatgaac cccagacgtg
     2041 cgcgcaacga cggagcaaag gcttgtccca agttccccac cgcgccttcc gcactcaaga
     2101 ccacggggcg cccgggaaaa gcgtcgaacc cccaggtcaa atccacgccg tgaacgcagc
     2161 cctccccgtc gcagtagtgt tgcggaccca acccgctgcg aaattggacg gcgcgcgctt
     2221 cggcgaaccg aacggtcagg tgttcccgcc caatccacgc ctgatcggcg acccctgcgt
     2281 ccgcctctcg atacactgac cagtcggcat cgagctcgag acgatacggc aattgcaggc
     2341 gagctcgcac gccgttgcga aggatgccgt catgaatgcc aatcccgctt tctaccgcta
     2401 actgagccgc agcccactgc gcgtcgggcg ctcgcacggg cgcggcgcca aacacggtgg
     2461 gctcgggttg ttccaggaga tagcccgttg agccgggtgc ataagggtag tcggcaaagc
     2521 gagtccgttg ctcgccgttc tccgaacgcc cactctccac gatcgcatga ggaaacgtcc
     2581 acggtgacgc cagcacatag aacatcactc cgaaggcttg gccacccgag ctactgcagc
     2641 agctcgaaga atccgaataa tcaccctcgg aatcagagct tgccgaagag ctggctgagc
     2701 tcgaggacga gctagagcta tccgaacggc cgcgcgccgc atcaccggcg cgatcgagtt
     2761 tccccgccca aacggggtga ctcagcagaa agccaaacaa accggcggcc agagagagcc
     2821 tcgaaccgca caacccacga aacgtccgca taccgtggct ggccaaagca ccgcgcatgc
     2881 cagcgctcgg agcgcaaccc ctcgacccaa agcctcgcgg atcttcagca cgaaagagca
     2941 agcaccgcgc gtcgcccgtg taatcggccg ttcacctact gcatgtgccg ggagcgccca
     3001 gacgtaggcg cctacgtgcg acttccgcgc tttacgtcag gcgcgctcga ttcgaccgtc
     3061 gttcagaccc acacgcgccg cgcgcgcaac gcgcgcatct gcatcacgag ccagccggct
     3121 caaagtctcc gcatactgcg cggggctccg ggagtagtgc tgcgccgctg cgcgcgccgc
     3181 gtgcaaccgc gtatggggtc gctcatcggt tgccaacgta tcgatcacga aatcgctgac
     3241 cggcgtgtgc acgtggttca gcaaagcgcg cgccaacacc tcgcgttgat tcggctggtt
     3301 tgccgtagcc cactgacata gcagctcgat gcgctgaccc ggatgcatga ccgccagcgc
     3361 gcgttcgagc ccctccgcgg cagccacacg gacccgcacc gattcgtcac gggcgaggcg
     3421 ctcgatcaag ctcaacgacc gcgacggcgc aattttcgcg agcgccccga gattgcgtgc
     3481 cacgcgagcg cgcacccgcg gccgaggatc ggtggataac aaccaaagca cgcggctccg
     3541 agcctctcga ggccactccg aaggctcgaa cgccacctca tctgcaactt cggaaaccag
     3601 agtttctgga atggccaaca gacgtgagtc agaaaactcc gcgcctgctg cggccgccaa
     3661 ggcgagggcg agtagatact tgaacaagcc tttgcgtgat ggcttttgca tagcaatctc
     3721 cacgtccacg agatcggtcc gacgacgaag ggttacgagt tgcgaggagg gcgactcaca
     3781 actggtgtgc gcgaaacccg cgcttcgggt cgactattcc ctgcgtgggc caataaaatc
     3841 attctgaggt aagttggcgt cgcgcccgtg aacacatctt cccaactcga agccaacgcc
     3901 ttccccatcg agcccgcgac gcgagcgacg ccggaacgtc acgtcgtctg caccgaagca
     3961 ttgagcttcc tcgccgctca ccctgccgaa agcggcagct cggtgattac ttcattgccg
     4021 gatgtttccg agctaccgga gttgggcttc gagggttggc gggcttggtt tttgcgagcc
     4081 gcgcgctcgg tgctcgcctg gacccccgag gatggggtca gcatattctt tcagagcgat
     4141 atccgttacc gcggtgcttg ggtggacaag gggtacttgg ttcagcgagc cgccgaggat
     4201 ctggggcagg agctcgtgtt ccacaagatc gtctgccgaa aaactccggg caccatcacc
     4261 cacgggcgcg cgagctactc gcacctgctt tgtttcagca aacgaagccg cggcatgccg
     4321 cgcgcaccgg gccccgacgt gctcgcggat gccggggcaa tgtcgtggag caaagcgatg
     4381 ggagagaatg cctgccgtct tgcgtgtcgg tacttggcct ccgaaaccgc cacgcgcctc
     4441 gtggtggatc cattttgtgg acagggcacc gtactcgccg tggccaacga gttcggcttc
     4501 gacgcactcg gggttgatct cagccagcgc aaatgccgtg ttgcgcggcg tctgcaagtg
     4561 ggagttccca aaccagcccc agagctcgac gagccccgcc aactcgagga gccccccacg
     4621 tagcggttct cgcgagggcg cgatcgcctc gaactcacag tcttcctgcc cgctggccga
     4681 gcctcgagca cgcataaccg cgcttgaatc ccgcccaaac ttcatgcggg cgccgaaaaa
     4741 cggatgctat aagcgcccgc gtgccgcccc ttgctcagga tctgagagcc gctgccaact
     4801 ccgtgatttt cccgacgtgg accgcattat gcgtgtccgc tggcatctgc gccggcgcgg
     4861 tgacccgcga ttcggacttg ttttaccgct ggcaacggca ctgggcgcgt gggcccttcg
     4921 tgctgtgcgg aattcccctg cacgtgagcg gccagcaaaa catggagccc gaccgtggct
     4981 acgtggtgat tgcaaatcac tcgagctaca tggacatccc ggccgtgttc gcgagcctac
     5041 ccacgttacc gagcttcttt gccaagcgtg aactcagccg cattccattc ctcggccagg
     5101 ccttgcgttc cggccgccac atcctcgtcg accggggcag ccgttcgagc gcacgagata
     5161 ccctggagcg ggccgcggcg cacgtgcgcc gcggcggaag tgtcctgatt ttcccggagg
     5221 gcacgcgcca aacacgcgac gcgatcggaa cgttcaagag tggtgcgttt cgcctggcca
     5281 agctggcgca agcacccatt ctgcccgttg gcattcgggg cacacgacac gtcttaccaa
     5341 agcacgggcg tctgttgcgt tcacacccgg tccagctaca aatcggcgca cccatctcgc
     5401 gcacggaagt gctggatatc gacctgggaa gtttgacgcg gctttgccgc gccaaggtcg
     5461 ccgaactggc ggggcttccg attgaggagg ccaccggcga aagcggcgaa accgctacgg
     5521 gctgaaccca tgcgcccgca cctgctcgca ggccccgtcc gggagctgcg tgagtcttta
     5581 ccccaggcgc tagcgggagg ggcgcagcca atcgatccca cgcagtcagc cctcgctcac
     5641 ctgctgcgca gcgcggccga ccgcgtctac gcggacctgg tgtcgcgatt cggacctggg
     5701 ctgctggact cgttcgagcc gccgtcgtcg gacttggcct cgagcgcgga gtgggagcgc
     5761 atgctcagtc agcggctcgc tgctgactca ctcggaccgg cttggctaga cgacacctct
     5821 ctgggttgga tttaccagtt ctggcacgag gcggatcgca ttgcgatcga tgcccgtctg
     5881 gatgcgcaac acaaactgga gccgcaggag atcgcgacca agacccaagt cttcaccgag
     5941 cgctacatga tcgattggct gctccagaac agcctcggaa cgctttggac tcgcgtctgc
     6001 caacggcacg gctggcgaag ccaggtgctg gcgcgttggc cctacttcaa ggcttgcccc
     6061 gagcccgact cgaccgaatc tctggcgctg ccgcagcgat tgaatgaact aaagctgctc
     6121 gacccggcct gcggagccgg gcactttctg gtggtcgccg tcgactatct gctgagtttg
     6181 tatcgcgaag aagccgtgct ctccgggcaa ggcggcagcg atcgcgagtt cgtagaatcg
     6241 atcctcgaga acaacctgtt cggaatcgat ctggacgcac gtgcggtgcg tgtggctgcc
     6301 tcgagcctgc tcttgaagtc gctgcgcgta gccagcgacg cgcgcccgcg ccgtctgaac
     6361 ttggtgggca cggagttcgc gaccgagctc gcgcgcattc agggtcgaga ccccctgtat
     6421 gagcagctcg cggaggcgct ctctcgcgcc aaagcgcccg ccacgacggt gagccgcgtc
     6481 ttggcgctgc tgtcgagcgc ggcgcacttg ggcacactcc tgaaagtgaa tctcgagctc
     6541 gacgcctggc tcacccactc cccccacggg ccggaggtgc gccgcgcgct ggagcaattg
     6601 ctcacgaccc acactcgagc tctcggcggc gcgcgcgcac tggatgccgg gagcgaaacg
     6661 ggcttcgggc ttcgcgcgct gcgcgctcag gagccggacg gactgctgtt ctggcgcttg
     6721 acgaacgagc ggcagtatca cgtcatctgc ggcaatccgc cgtacttcgg aacccagtcg
     6781 atcgcggacg ccagctattt ggatcgtcgc tatccggagt cgcgcgaaaa cttatgcacg
     6841 gccctgctcg atcgagccat ggagttggtg ctacccggcg ggctcctcgc gttcgttacc
     6901 caacgcaatt ggctctacgt caggcagctc cagtacttcc gtcggcgtat gctggagacc
     6961 ttcgctccgt tccgcgttgc cgatctagag ctcggcggct tcgagtcgct caagaccgta
     7021 gacggtattc tttgggtagc ccgcgggggt tcttctggaa gctgctgggt cacgagagcc
     7081 cccgcgccgg agccaacctc gaaggccgcc gcgctgcgtg agcctcgcga gacttacgtc
     7141 acccatccga gtctgctcgc gcgtctgccc gggagcccgt tcgtttatcg ctggccccgc
     7201 gagttcgccg aacgatactt gtccgagccg ctcctagggg agcgttatcc ggtccgggtc
     7261 ggcatgaaga cctcggacaa cctacggttc gtgcgctacc cctgggagct gccggccgcc
     7321 gtgactcgac gagccgccga acagcccgcg ggcgcagact gggtgcccta tatcaaagga
     7381 gccgaaggca agacctggat cgaaccgctc tcgcacctgg tttgctggcg agaacacggg
     7441 ctcgaaattc ggatcgcgct cgaggccgcg tatcaacaga agccccaagc cgagaagtac
     7501 ttcttcaagc gcggggtggc cttcaacacc atagggcgaa gcttcgtcgc gcgggcgcac
     7561 cgctacgcga gtttgttcga tgtagctggt tcgtcggtat tcccggacga tttggccgaa
     7621 gcagtgtgcc tactgaatag ccgcttcgcg cgcgaaatcg tggaaagcct caacccgacg
     7681 atcaacttcc aggtgggaga cgtagcgcgg atcccggcgc gcagagatcc cgaggcacga
     7741 gcgttgttcg cgttgttgga gagcgccttc gagaccttcg aacgcagcga tgagaccagc
     7801 tttcgctttc agcggccagc ggcgaccacg tggatcggag cgcaggcgta cgcggcgcgc
     7861 gccgtgaatc gcccagaggg cgcggccttg ccagagtacg agcccgatgc tcggccgccc
     7921 acacccgaag ccgcgttctc gtatgcattc ggagttgcga tcgggcgctt cgacgcgcaa
     7981 ggcaaaggct ggctcgacac ggctccgtcg agtgccctgc cgcacgggct gctgttcatt
     8041 ggtcaactcg gccacgatag cttgcagcat cccgcggcgc gcggggttct ggagaccttc
     8101 gcgggtttgc gggcttcgcc aacggctcca caatcgggta cggcgccctc atccgagcgc
     8161 gagctttcgg ggttacgcaa gttcttgcgc cgggagttct tcacctcgca tcgtaagcaa
     8221 tatcggaacc ggccgattta cctgccgctt gcttctagcc aagccaactt cgtggccttc
     8281 gtgtccgttc atggcttcaa cccccgaacg ctccgcgcgt tggtggatga atatctgacg
     8341 ccagagctcg cctgcatcga ggcggcgctg gtcgcctccg agccaccgca cgcagcaagc
     8401 gagcagccgc gtgccagtgc cgcgagctcc cgcagcggag cggagctcgc gaagctccgc
     8461 tccgagctcg tctgctggat ccgcgacatt ggaaccgtcg cgtcggtggg cccgtggtcc
     8521 ccagacgcgc tgacgcctcc gcgcgaggtc gacgccgagt tcctctggga aatggatgac
     8581 ggcgtgctcg tgaatagcgc ggccctgtgg cccctgctcg cgccgctctg gtccgagcca
     8641 aagaagtggt ggtcgcaact cgcacggcgc agcggaccca aaggttgtca cttcgattgg
     8701 tcccgcacgg cgcgtcgtta ctttccgcaa cgggtggagc gcgcttgcgc cgccgatccg
     8761 gtcgtggcgt ccgcccacgg tgagctcgag cgctaccatc cacagctcgc cacgcgttgg
     8821 cgcgaacgcc ttcaggagct cggcgaccag acaggaaatc caggctgaac cgcagcctca
     8881 cgatcgagga caggagctgt cctcgatctg cttcgagtag ctgaatccgc gttgagctcg
     8941 cgcgcagcgc gcgataatct ccgagtcgat gcccggcacg tcggcccgct tgttgcgcct
     9001 gctcgcgctg ctgcaggccc gcaaatattg gccgggcccc gagctggccg agcgactcga
     9061 aatcacgacc cgcacgttac gtcgagacat cgatcggctg cgcgagttgg gctaccccgt
     9121 acaagccgcg tcgggcgtcg cggggggtta ccagctcggc gcgggcgcga cgctcccccc
     9181 gctgctcttg gaggatgacg aggcgctggc cgtggcgctg ggcctactca ccaccgcgag
     9241 cggcgcggta gtcgggctgg acgaggcggc gttgcgggcg ctcgccaagc tcgagcaggt
     9301 gctcccttcg cgcctgcagc ggcgcatggg ttcactccgg gcctccctta catcgctcga
     9361 gagccacgcc cccaaagtgg aggcttccgt actcggcagc gtggccgccg cctgccgcga
     9421 tcgcgatcgc ctacagtttc gatacgaaga cgcgcgcggg caaagccgcc aacgcgacgt
     9481 agagcccgct ggattggtgc acatgggtgg gcgttggtac ctcgtggctt gggatttgga
     9541 gcgcgtagcc ttccgcacct ttcgcctgga tcgcatcgcc aagaaggtga gcctcgggcc
     9601 acgtttctcc ccgcgggagt tgcctgaagg cggagatttg cgagcctacg tcgcgcgcca
     9661 ggtcacgtcg gtggtttacc cggtgcgcgc cgaaatcgag ttcgccgccc ccctcgccac
     9721 gctgcgtgag cgcgtaccgc ccggggtcgg catgctcgag gccttggatc gagaccgctg
     9781 cctcttgaag accggcgccc attcgatgtc ccacttggcg ctgcacatcg cgctgctgca
     9841 ggtggacttc gtggtcaggt caccgcccga gatgatcgag tacctcgagc ggctgcgttc
     9901 acgcctggac cgcgctttgc ccgcgagttc gagcggctga agtggtgcgt cgcgcgcgac
     9961 cgaaaagcgg atcgcgccgc gtcaatctcc ttcgtattca gggcttctcg gcgagcgtga
    10021 catcgtccag gcagagctcc tgattgttgc caatcgaacc cgtgaatgcc agcccgagca
    10081 caccgagccc ggcgtcgtag tcgaactcct gcgaatacgt ctggtaagag ttgccgaacg
    10141 agaagctctt cgacgtcgcc gcatcgtacg gagcttccat ctggcccacc ttggcatcta
    10201 cgtccgtggc cgcgctggct ctcgcccgga acgagaacac gtagtgcctg ccatcattca
    10261 aggacaagcc aggctcaccc tccttcggat atccaatcgt gaacgagagg gcgccttggc
    10321 ctttcaggca gtactgccca ttggtcgaca gcgagatgcc tcccccgttg tgcgactcca
    10381 ctttccaatg cgcctcgccc tgcgcgaaat caccgttgcc aactagatta ttgccggaca
    10441 tgccgcccgt catgttcgaa gcgccgccgc tcgacgagcc cccgttagcc gtagttccgg
    10501 ctgtcgtatt ggtcgagctg cttccgccgg cggaagtgcc accgagctcc ggctctcccg
    10561 tcgctccggc ctgaccgttg gtagttccgc cggatgtcgt cggctctttt gccccggcct
    10621 cgcccgtgga atcagaacca gaacgcccac cgctcgcgct ggtttgccca cccaatccac
    10681 tcgtcgtccc cccggtctcg ctcggtggca cgacgcaacg attcgagaag caggccagtc
    10741 cgtcgttaca agtgttgttc gggtaacatg gacagcgctc cgtccccgtc gtgcatgaac
    10801 ttccggcctg ctcgctcgaa cagccgatag tgaggtgaaa cccggagaaa actagggcaa
    10861 gcgtgaaact cgatcgcaac ctggaaagca tcgcagacgc agcatgctca atttccggca
    10921 gcgtgcaaag tcgctcgccg gctttgcagt tcctggtaga gtgctgcact ctcggcacac
    10981 gagaacagcg acgatgtaag cgctgattcg gactttcgaa ggcccgctcg agagcaggtc
    11041 gtcgacgaac caagctcgag aacactggaa gatgcaggaa gacgctggtg cacgagaaac
    11101 cccggaggcc gtcggcgatg ttctggcggc ttttcgagaa atcgtggaca cggcggtaac
    11161 gccaacatgg ctgttgtcgc cggactcatc catgctgcac gccaacggcc cacttcacga
    11221 ttggtgcggc gcctctgcac gtgaactgca gggcgtgggt tggctgactc ggctgcatcc
    11281 cgaagacgcc gcccacagct ccgcttactg gcagcgtcta ttcgccgacg gtcagagcac
    11341 cgagatctac tttcgcttac gacgtatgga tggcagttac gccggcctat gcgcgaactg
    11401 catcccgctc cgagacggca gcggcgcctg gctggtgacc acccgcctcg agagccctgc
    11461 ctccggtacg gctgcggtgc tcacgcgctt ggacccgaat gccaacgggc acgcgctgcg
    11521 cgcgctgttt ttcgagcata tgctggagcc cgcgttcatc tgggagctca gcgagaaacg
    11581 cccaatcatc gattggaacc gcggggccga gcaaaccttc ggtttcgagt cgcaagaggc
    11641 catcggccgg gacgcgcggg agctcctgca aacccgtgca tgcacccgct ggagtgaaat
    11701 cgaagctagc ctgaagcgag agggatcttg gcaaggcgag ctactgcaac ggcaccgtct
    11761 cggggatacg ctcgcgatcg agacgcgtct gcaactcgtg ctgtgcgcag gccgcccgcc
    11821 actcgtgctg gagagtggga tcgataacag cgctctgcgg caaggagaac gcgccgtgat
    11881 cgagggtcag gcgcgcctgc ggctcgcact atcctccgct cgaatcggga tctgggaatg
    11941 gcggctcgag tccgatcggt tggtctggtc ttccgaggcc aaaaccgtct tgggctcccc
    12001 gcccgaggag ttcgcgccta cgctgcctgc gctgatcgcg cgcgtccacc ccgatgaccg
    12061 cgccagcgtc cgggagcgcc tcgaagacgc ggtcgcgcac gtacgtgacg tcgaactcga
    12121 gtttcgttgc cctcggcacg accacgtgcg ctggctgcgc tgtcgtggaa tcgtcgaacg
    12181 cgacgaaggt ggaagagcaa cccgcgtggt cggcacgatc tcggacgtga cccccgaacg
    12241 actacaagag gctagcctgc gcgagagcga acagcgcgca cgcgctcgcg ccgacgagct
    12301 ctctgccctg atggacgcgc tgcccgcagc cgtctggatc gcccacgacc gccatgccaa
    12361 gcaagtgacg ggcagtcgcg tcggccacga aatcttacgt atgccgtggg gcgcgaacct
    12421 gtcgaagact gcagaagata cggctcccgt ccagcatttc acggtgctca aagatggcct
    12481 cgagctacaa ccggacgagc tcccgttgca ggtcgcggca ttgaccggca aagagatccg
    12541 cgacttcgaa gaggagatcc gcttcgggga cggcagctcc atctacttgt atggcagcgc
    12601 ggtaccgctg ctggacgaaa agcgagagcc gcgcggggcg atcgcggcgt tcatcgacgt
    12661 tacccgcatc aaacacgccg agtccgagca acgcgcggcc gctcgccgca aggacgagtt
    12721 cctggccatt ctctcccacg agctccggaa cccgctcgcg ccaatcctga ctgccgtcca
    12781 cctgatgaag ctgcgcggcg acgtaccagc gccgaacgag cgcgacgtga tcgagcgcca
    12841 ggccaagcac ctggtgcgcc tggtcaacga cttgttggac
//
LOCUS       DASTKU010000572         3547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_334327, whole genome shotgun sequence.
ACCESSION   DASTKU010000572 DASTKU010000000
VERSION     DASTKU010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334327"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFQ61_19835"
     CDS             <1..322
                     /locus_tag="VFQ61_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956761.1"
                     /translation="RQRAPRHRREGRRHDLGGDAHPPAPPLPIPPPSTPPPIPPLAPP
                     PVPPTALPPALPLELPATPPLGLLLAPAEVPPALVPPALAPPEFELVPALELPPLELF
                     VLPP"
     gene            complement(595..1236)
                     /locus_tag="VFQ61_19840"
     CDS             complement(595..1236)
                     /locus_tag="VFQ61_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956762.1"
                     /translation="MVDIIDAAPTGRAKCRGCGRPIAKGEMRFGERLPNAYGEGEASF
                     WFHLACGACMRAEKFATAWAQTDLTPIDRELIEGLNALARQFPRLERFAGVERASSGR
                     AQCRACRELIEKGSWRVSLQIYEDGRMNPIGYVHVACASEYFGTAEFLRVMASLESGL
                     SPADLAEITRLGSVPRLAKARPEHMESSNEGQSPSSSEDSSESDARRSEPKTG"
     gene            complement(1408..2259)
                     /locus_tag="VFQ61_19845"
     CDS             complement(1408..2259)
                     /locus_tag="VFQ61_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956763.1"
                     /translation="MSRSLASYCSSMFVILIAGGAWAQPAIPPAPSQAGPASAGASEV
                     PAGDAGAEVNLRAEAPPAPSYTAPAPSSATSVGTPAANTSATSATSSETAPATAANAA
                     PPSLMGKKVHFSGYGGVDVLYTRMFGRDGAVVGLQGALLLDHKLALGIAGYGWTNPER
                     GPSTDEGVSRRYQTVYGGFTAHYSFYWDSPAYLTVGALFGGGAVALTPTHSSRHDDDY
                     ERDHRVSDTFVVLQPDVTMHLNLTPWLRAGVTAGYRITSGVGYFGFQETDVNGLVVGA
                     SLQAGNF"
     gene            complement(2440..3153)
                     /locus_tag="VFQ61_19850"
     CDS             complement(2440..3153)
                     /locus_tag="VFQ61_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956764.1"
                     /translation="MRNSYCRLMLTLGVLGVVACGGSTDDEPKTSTGGVAGQSAQSGG
                     RTGSGGSSSSTGGSSTSTGGKSTGGASTGGRTSGGGSSGGSTSSGGDDTRTFAELTDD
                     ERNAVCQAEAEKLDTPENKAQNCKLLGAFAAFQVLQDAEQMPAACQAAYDLCMDQEQS
                     AQVECSDDLRECTATVAEFQACWDQLPAWFGALDVPSCEDVSLQTLLPLATLLTSLPQ
                     ACQTLQDKCPEAVPDPTEM"
BASE COUNT          636 a         1234 c         1033 g          644 t
ORIGIN      
        1 gcgtcaacgt gctccgcgcc accggcgaga aggacgacga cacgatctcg gaggggacgc
       61 gcatcctccc gcgccgccgt tgccgatccc gccgccgtca acaccgccac ccattccccc
      121 gctggcccca ccgcccgttc cgccaacggc actgccaccg gcattgccac tcgaactacc
      181 cgctacgccg ccgctaggat tgctactcgc acccgcggaa gtacctccgg cactcgtgcc
      241 acccgcgctg gctcccccag aattcgagct ggttcccgcg ctggaattgc caccgctcga
      301 gctattcgtg cttccgccct gagcaaccac gccacccgag gcgcgcgttg tgccatcggt
      361 cgtgtttacg ccgcctgtcg ccgtggctcc gccggacgcc gacggagaac taggctcaga
      421 gctgcacgcc aagaggggcg tgagggccag cagcaaagag acggaccatg aggaatcgct
      481 cagccgaatc ttcatcgagg gtggtctaac atgatgttgc acccccggct tggccgttct
      541 tcaagatctc ttcatcgatt ccgctctctg tgctcgcccc gcccaggcgc gtcttcagcc
      601 agtcttcggc tccgaacgtc tcgcgtccga ctcactcgag tcttcggacg aactcgggct
      661 ctgcccctcg ttcgatgact ccatgtgttc aggccgggct ttcgccaagc ggggcacgct
      721 gccgaggcgg gtaatttcag ccaaatccgc gggggacaag cctgattcga gactggccat
      781 cacccgcaag aactcggctg ttccgaaata ttccgaagca catgcaacgt gcacgtagcc
      841 aatcggattc attctcccat cctcgtaaat ctgcagagag acacgccaac tccctttctc
      901 gatcaactca cgacaagcgc ggcactgagc tcgtcccgag ctcgctcgct ccaccccagc
      961 aaagcgttcc aggcggggga attggcgggc tagggcattc agcccctcga tcagctcgcg
     1021 gtcaattggg gtcaaatcag tttgcgccca cgcggtggca aacttctcgg cacgcatgca
     1081 agcaccgcac gcgaggtgaa accaaaagct cgcctctccc tccccgtagg cattcggcaa
     1141 ccgctcgccg aagcgcattt cgccttttgc gattggccgc ccacagcccc ggcatttggc
     1201 gcgccccgtc ggggctgcat cgatgatgtc gaccaagcga gctccttttc tagcttcgcg
     1261 gaggattttg aggccaccga tcggttgtgg gcaagctttt gacggctcct ctattcaccg
     1321 gcgcgttgcc cgctccggcg tttcacccgc cgcgcccccc cctgcgcccc caccacccga
     1381 ggggccccgc gaacgtgacc ctaagattca aaagttaccg gcctgcagac tcgcgccaac
     1441 gaccaggccg ttcacgtccg tctcctggaa gccgaaatac ccaactcctg acgtgatgcg
     1501 gtatcccgcc gtgacgcccg cgcgtagcca gggcgtgagg ttcaagtgca tcgtcacgtc
     1561 gggttgcaga accacgaagg tgtcggacac gcggtgatcg cgctcgtagt cgtcgtcatg
     1621 acggctgctg tgcgtcggcg tcaacgcgac tgcgccgccg ccgaatagag caccgacggt
     1681 caggtaagcg ggtgagtccc agtagaagga gtaatgagcg gtaaatccac cgtacacggt
     1741 ttgatatcga cgggacacgc cctcatcggt gctcggccca cgctcggggt tggtccagcc
     1801 gtaaccggcg atgccgagtg ccagtttgtg atccagcagc aacgcgcctt gtagtccgac
     1861 cacggcccca tcacgcccga acattcgcgt gtagagcacg tccaccccac catacccgct
     1921 gaagtggacc ttcttaccca tgagtgatgg cggcgccgca ttcgcggcag tagccggcgc
     1981 cgtctcactc gatgtcgcgc tggtcgcgga cgtgttggcc gcgggagtgc ccacgctggt
     2041 cgcgctgctc ggcgcgggag ccgtgtaact cggcgcgggc ggcgcctccg cccgcagatt
     2101 gacctctgcg cccgcatctc cggcagggac ttcgctcgcc ccagcgctag cggggccagc
     2161 ttgtgagggc gcgggaggga ttgccggttg cgcccaggcg cccccggcaa tcaaaatcac
     2221 gaacatcgac gaacagtaac tcgctaaaga acgggacatt tgtagtccag cccaagcaag
     2281 agccgcgccc gaaccacgcg caccatcgag catctaaatt tgcctcgatc cgcgtcgagc
     2341 tgtgaatagc ggttgacgct ccccacgcgg acccgagaac gcgcgttgcg agcaacgcgc
     2401 gggtccccgt tcgagtgttc gctcttcgcc ggacggctgt cacatctccg tggggtccgg
     2461 caccgcctcc ggacacttgt cctgcagcgt ctggcaagct tgcggcaggc tggtgaggag
     2521 cgtggctagc ggcaacaacg tctgaaggct gacgtcctcg cacgagggca cgtccagggc
     2581 accaaaccac gcgggcagct gatcccaaca ggcttggaac tccgcgaccg tcgcggtgca
     2641 ctcgcgcagg tcatcgctgc actcgacctg cgccgactgt tcttgatcca tgcacaggtc
     2701 gtaagccgcc tgacaggcgg cgggcatctg ctccgcatcc tggagcactt ggaatgcggc
     2761 gaacgcgccg agcagtttgc agttctgagc tttgttctcg ggcgtgtcga gcttttcggc
     2821 ctcggcttgg catacggcat tgcgttcatc gtcggtcagc tcggcgaagg tgcgcgtgtc
     2881 gtcaccccct gaactcgtgc tgccgccgct gctcccgcca ccgctggtac ggccgccagt
     2941 gcttgcgccg ccggtgctct ttccgcccgt cgacgtggag ctcccgcccg tgctggagct
     3001 gcttcccccc gagccggtgc gaccgcccga ttgtgcagac tgccccgcca ctccaccggt
     3061 gctcgttttg ggttcatcgt cggtcgaacc accgcaagca accacaccta gcacccccag
     3121 cgtcagcatc agtctgcagt aagaatttcg catctctggt cagctccttg gaagcgtcga
     3181 agtatacgca gaagtttcag ggcgtccctc ggcaaacaca gttccgaaag cttccgccga
     3241 gtttttggcg gaaactatcg ccgcgcttcg tgtctggcgc acccgtaaac acttattgct
     3301 gtgccttcga ggcgagacaa gctcgagcga gctggccttc gggcgcgaga accttcgggc
     3361 gcgagaaact agtgccacga aaagtcctgt tgcaggcagc gaagagagct gacttttcag
     3421 ccagcgcacg cgccggccca cgactcctgt ttaccaagcc aactgctgtc gaaagtcagc
     3481 agcgcagtcc gcgtctcgaa acgaccaagc gagcgcgtca tcgaacgatg aaagccaggc
     3541 atccaac
//
LOCUS       DASTKU010000573        29976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1897833, whole genome shotgun sequence.
ACCESSION   DASTKU010000573 DASTKU010000000
VERSION     DASTKU010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 29976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29976
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897833"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="VFQ61_19855"
     CDS             complement(<1..1201)
                     /locus_tag="VFQ61_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956765.1"
                     /translation="MNAWFRRLMLAVTLPLGVLGACDTGGLVGGKCRSGMLFCNSECV
                     DPSTDARHCGACNNTCSDGASCVDGQCTSSTGGVDNSGGRSTGGNGSLEGGASGAGDT
                     GGYTGQPYPVGGDSSGGTNSGGTSARGGVGGTGGTRPRPEAGSAGVSCDPPFDTSQHC
                     GDCDTSCVDPNPLCSPAEMGGFECVPACPPGLLACGDQCIDPNVNPLHCGECFNLCPS
                     GICQAGMCVGASPGHVALYCMNYSWAQPETAHTTLLGNAVYLPVRARVRVLSFSRWAS
                     ANAVSQIEKVVRWSGEARGRTAQVDEMRDPAQLSARLSVQNYDVFLIQEQGNATRDEL
                     IGLGATWASTGVVDSFAKAGGVVVVLGADANDGKTAALINSLGLLDMVGQTPIALDDV
                     TTRFYNRA"
     gene            complement(1198..2793)
                     /locus_tag="VFQ61_19860"
     CDS             complement(1198..2793)
                     /locus_tag="VFQ61_19860"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9956766.1"
                     /translation="MSWPALAEPPRLHGSAAATRALGGYQEREYGWGLAFMPSVELPI
                     TRAFGLQAEFAGIWLTQGDPPKNPYIEPQGEGSGLSGGVGPRLRPFAASHDGTRISPA
                     GFWLGATLGAAYTNELLRPMFNAEIGYDFLDSPGKVGMGPMLGFYHVMQPNNQVRPED
                     ANLVLVGVHALFDTGSGLRRDSDRDKDGIRDELDRCPDDAEDRDGFEDTDGCPEADND
                     RDGVLDLADHCPAQPEDRDGFEDDDGCPDVDNDKDGILDTKDRCPNEAEDKDEFEDED
                     GCPDTDNDKDGIPDVKDLCPFEPETINDYADSDGCPDEDQVRVVGDKIVLDDRVHFMI
                     NSHIIRKESFELLERLAKLIVDHPEYIHISIEGHADERGSEEFNEKLSRGRAYSVLEF
                     LVKSGVHKNRLSHAGYGSKRPLVDSKGESAYYQNRRVEFIITREARVSGKSPLPPKPP
                     SLGSDGPPPESEQPEMKPSSDSGATQPQSENPGRDSKPPGLALPKAPVLAPPSETTNN
                     ASGSAPTAKPTPKSDGTNTRGEP"
     gene            complement(3050..3388)
                     /locus_tag="VFQ61_19865"
     CDS             complement(3050..3388)
                     /locus_tag="VFQ61_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9956767.1"
                     /translation="MPRVRFVDQNIEVEVPVGSSILAAAQGSGAPEGSACGGVCACST
                     CHVYVEAGADLLSPAEEGEEDILDKAFDVRMSSRLGCQARLQRDGLVSVRISKESLDA
                     FYNEHPNERR"
     gene            complement(3388..5307)
                     /gene="hscA"
                     /locus_tag="VFQ61_19870"
     CDS             complement(3388..5307)
                     /gene="hscA"
                     /locus_tag="VFQ61_19870"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HET9956768.1"
                     /translation="MVLLDIFDPKAPPKAIGIDLGTTNSIVATVRDGRPLALTNCDGT
                     ALLPSVVYYAREGNVIVGRAALAYATREPERTIQSVKRFVGRGANDAETQRLSAYKFA
                     RPETEEEAKVVRFDLGELQASPVEVSAEILKSLKQSAVDQLRNVGPAVITVPAYFDDA
                     QRQATRDAGRLAGLEVLRLLNEPTAAALAYGLDKQQNGLFAVYDLGGGTFDVTVLRLE
                     DGVFQVLSTGGDSALGGDDMDRAVADRMLTALDAHSRARSSKELWSVVLASAREVKEA
                     LTEQQTVEVELPDASNRSRIFTLTRGELDELIQPLLERTGKACRRALRDAQVAPEQLD
                     GVILVGGATRVPRVRSYVQELFGKPPLADIDPDLVVAYGAALQAEMLVTGSDDVLLLD
                     VLPLSLGIETMGGGVDKILPRNTTIPAGARATFTTYADNQTGFEIHVVQGERELAKDC
                     RSLARFTLRGIPPMPAGAARLDVTFHVDENGLLSVTASERTTGQSQTVEVKPSYGLSD
                     EQIEKMLEDALDFGEEDFAARRLADARVEGERVILATQKGLQQDPDLLAPAERERIDS
                     AVAALKEAIVQSSDGALVQARIDALADATHEFAGRRMNRAIGLSVSGKAVQEVEQRVQ
                     HAAGVEAHLREKGLT"
     gene            complement(5387..5950)
                     /gene="hscB"
                     /locus_tag="VFQ61_19875"
     CDS             complement(5387..5950)
                     /gene="hscB"
                     /locus_tag="VFQ61_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HET9956769.1"
                     /translation="MDPFELLAVEPRFDLDFGAAEARHRELSSALHPDRHVRGSSVER
                     RRALSRAIEVNEALRILKDPVRRAEALLERYGMVASESEAVASPDFLMEVLELREELA
                     QAQRRHDVAAIQRLVENVRGRESAVEQRLIQAFRSLSAGALEANTADGGSPPIGATRE
                     RIAKTLGELRFFRRLVGEAHAILDDLD"
     gene            complement(5998..6375)
                     /locus_tag="VFQ61_19880"
     CDS             complement(5998..6375)
                     /locus_tag="VFQ61_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET9956770.1"
                     /translation="MTSAPSEAPASGRSKRSIFVTEEAARFARKKLDARGTPDAVVRL
                     GIRGGGCSGFSYVIQFEDEAPRERDNVFEFGDVKFVIDKKSLIYLAGATLDYEKTLMF
                     QGLKFKNPNEASSCGCGHSFTVK"
     gene            complement(6435..6845)
                     /gene="iscU"
                     /locus_tag="VFQ61_19885"
     CDS             complement(6435..6845)
                     /gene="iscU"
                     /locus_tag="VFQ61_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HET9956771.1"
                     /translation="MAYSEKVIEHYENPRNVGMLDKDDPTVGTGLVGAPACGDVMRLQ
                     IRVNEEGVIEDAKFKTFGCGSAIASSSLATEWIKGKTVDEAASIKNSQLAEELNLPPV
                     KIHCSVLSEDAIKSAIEDYKKKRAAREALSEQKP"
     gene            complement(6904..8127)
                     /locus_tag="VFQ61_19890"
     CDS             complement(6904..8127)
                     /locus_tag="VFQ61_19890"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HET9956772.1"
                     /translation="MALKFPIYMDNHATTPVDPRVLEAMLPYFTQEFGNAASRTHAFG
                     WHAEGAVEAARETLADLIHAESGKEIVFTSGATESNNLALKGVAEYYSGKGNHIVTST
                     IEHKAVLDSCKRLEKQGFQVTYVNAGKDGIVDPGDIQKALTEKTILVSVMLANNEVGT
                     IQPIEEIGQITRARGVLFHVDAVQGLGKTPFDVRAMNVDLASLTAHKIYGPKGVGALY
                     VRRSRPRVRLVAQMDGGGHERGNRSGTLNVPGIVGFAKAAEILKAEGEVEAARLRALR
                     DRLYGKLSRELDQIILNGHPERRLPGNLNVSFAFVEGEGLMMAIKDVAVSSGSACTSA
                     SLEPSYVLRSMGLDEDMAHSSIRFGLGRFNTEEEVDYVADLVVSKVKKLRDMSPLWEM
                     HQDGIDIKSIQWVAH"
     gene            complement(8137..8757)
                     /locus_tag="VFQ61_19895"
     CDS             complement(8137..8757)
                     /locus_tag="VFQ61_19895"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9956773.1"
                     /translation="MDSNTPADKRLRLVVLPQAVGLAGVPTRPRSDVTAADPSADGNG
                     LGAAASEELLQVGELAKAAGKTVRAIHLYEELGLLRAHERSKGRYRLFTEEALVRVRW
                     ITKLQNLGLSLSEIQELVREQEASGSAQFAATRLREVYRAKLEETRTKLSELRALEAE
                     LTDSLAYLDTCQTACETELPVHSCPKCARHPERPAVPELVAGVHVS"
     gene            9156..10109
                     /locus_tag="VFQ61_19900"
     CDS             9156..10109
                     /locus_tag="VFQ61_19900"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET9956774.1"
                     /translation="MPNPLRRSALCFGIVVSVSVLGAARPTGSAARPTKAPKTPVGTG
                     APRRLSYEEAQLFVLSLINRDRAKAKLRPVQMDDAASRAGQRHARDLATHGFTGHVGS
                     DGSVPEQRYTESGGQHFVQENAACISDTTLRQLAPNALYDSSKLAALHQMFMSEMPPN
                     DGHRRNILNPSHNYVGIGLALPADLPQPCLTQEFVDQYGHYEALPSAAKRSAKFRVAG
                     TVSEPLLFGGVGLGKTPLPVPPPKRHLNGNLYRIPAPDKMFFPAGFKTPKPVSVDGPR
                     FDINLDLGKNAGPGLYAISIWAKQPGTTRLFMISMRTIRVE"
     gene            10368..11411
                     /locus_tag="VFQ61_19905"
     CDS             10368..11411
                     /locus_tag="VFQ61_19905"
                     /inference="COORDINATES: protein motif:HMM:NF019256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET9956775.1"
                     /translation="MQSSGGVASGGLTSTSSAENSSGSPIGDTPESSGGNSGPPSSVC
                     FVPESVSQDTDSKFVDPLEVRDRITRLLLGVPSSAYALPDTTTRAWAAELARQIARDT
                     VPLPFLRRFFAFWFDNDEAWNTQKPYQATSLALEWGDTWATTLAQHWSFQKLMTASTP
                     QARDVGLLTDTRLLSRYTEIANRGAWLTNKLLCLGAPPEPTNFRAPPPPEGITRRDHL
                     AAQVDHEPCRSCHHWFDPAGWALEHFDERGEYRTSDRGLSIDSAGELPLAFDDSKVTF
                     SSINDLSEQLVGSCAVAHCMAKTLTSMALNAAGVSATPSEAELQHIANEFRSEHSALD
                     MIAAIAASPSMLR"
     gene            complement(11455..12918)
                     /locus_tag="VFQ61_19910"
     CDS             complement(11455..12918)
                     /locus_tag="VFQ61_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956776.1"
                     /translation="MSLEVSVDVEQASPVVPGAPKLASQLLERAAAARLTAALRNLSA
                     QHEPGVRARFRAEIATIIEQAEQAWAVYQNELALLDIAAPLEVTTLREPQTERGLGRH
                     HSNERQVAAVAPRLGSTLCRAYAARAEDACHGANQLSLSAQRAPTREACEAGWQKVEA
                     IVRIAERAARDSAEIVVQLEHEGYAVAAASRAARSAEAAAKAARKVIADRNRSYTFHT
                     QSGFSFGEGWYLAAAAVLEGVLIQIEPGQDGTPTAEKFLLDAGLASQIRPYRARPCAM
                     KHTTESIARAFRADAITAQRRLRSAFLGNGPISGAVADWIEDRVQTASVSRMQQKKVL
                     LWIRDGAHHPGRNTEYLELLELTRRVQGAGLTPIWIGDALRDGSIPDGVIDMILFWKD
                     PIFRTVDMRRSQLQFFEYLIQRHGLLGQVGVTTAGMDGPALMGLRTLYLTQAPNPRMG
                     EWVGAVPGYREVVREAGYLEKISQELSVWSLRSGEQA"
     gene            complement(12915..14516)
                     /locus_tag="VFQ61_19915"
     CDS             complement(12915..14516)
                     /locus_tag="VFQ61_19915"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9956777.1"
                     /translation="MFRDCDVIEESKVHSYHGRRSIGRHARGQTRLMAILGVGVCIVS
                     AAGCGNSDGPQGPSVSGNTNSITGGAMGDGNTSGSGASATAGNGGASANSTGGGSTTG
                     GVAMTGAAGQSVTGSGGRSPSGGASGVGGQAQSGGQSGSAGAGASGGINQAGRGGIPG
                     DGGRAANGGSDTAGSGAGGNSTGATGGASGGASMGPPSCATAGPGRTTCGEKGDESCC
                     SSPTVEGGTYSRTYKNTGSGATEKADAATISSFRLDKYEVTVARFREYVKYLENGGSP
                     PAAGSGKHAHLNGGKGLADSGKSGAFEAGWDASWSSKIPSGAGATAKWKALLTAKGTN
                     ANSGCNIYGSWSDESGKNDSLPITCTSWYESYAFCIWDGGFLPSEAEWKFAAAGGDEQ
                     RAYPWGSTAPGASNDYAIWDCCYPSGQCSGASGRDTCTGLVNVAPVGFAKKGAGRYGQ
                     LDLVGSVWEWLVDRYADKYGSPCQDCAYLTGNTANRVLPGAGFHTGASLGATLMYSWN
                     RSQISYNADTYRGDYAVGLRCARSP"
     gene            15727..19602
                     /locus_tag="VFQ61_19920"
     CDS             15727..19602
                     /locus_tag="VFQ61_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956778.1"
                     /translation="MACPWALASLLVASLLAACGGDPEAPNTGDPYANGARGGTAGTD
                     SGGTSNRGGTGGVINIGGGNACVPHTCESLGTSCGTVADGCGEILNCGECEADGLCGL
                     LSPNVCATPDDLSTLCQPLSKKEACANKQCGIEGDGCGNVYECGDCPQGEACGLDEAF
                     QCAPAITDPTENCPARIESCEAADVECGVIGNGCGGIIDCDAEKGGCAAGQGCGVAEP
                     GKCGELPTCEPFTAAEACAGKCGLVSNGCGNDVDGGLIDCSAMFPCPNGQTCGGGGVP
                     NQCGTSANSCQPVAKTQACEGIECGAVGDGCTGSYSCGTCSGGALCVQGQCEQPSCSA
                     IPRATACAGKACGAVGDGCGGTYNCGTCGANQQCGLRTAFQCGTVTPTSCTPLTKQQA
                     CAGKECGTVYDGCGSAPANQINCGTCTTGQFCGLRQAFQCDAPQVPTCTPNATSCAAL
                     GWECGMAVNSCGTVYDCATEGRSCGPLETCVGGITGPARCETPAGLNCPLCAAVADCS
                     GRPQRTRLAGRVVTPGRANNDTGNQVGVPNAYVYILRTNNVADLPAVGSGIPAGGESC
                     DRCNDQDLGAVLASSVTSATGEFVIEGNVPVGQQFLLVVKVGKFRRAVQYTLPASAAC
                     TTTNLPTAVATNPTRLPRTMSDGLAVNIPRIAITTGQIDAMECVFEKMGIASTEFTNG
                     SATTSGPRVHLYRGGSSSTPRGASLNASTPHDTALYSDAARMRSYDMVVADCEGQTWD
                     SSFSQRDSYGGNVRDYVNRGGRLFASHLSFSWLHENGTAAYATGSARFATGLGPAATW
                     DPALYTDTSGLGRVAIGRPRASSRIQNFADWMINQGVIANSTATFTITDPRSNATGLG
                     PASEEFVYRTNGNQRTQQFSFNTPYSAPQAAACGRVAYSGFHVASTGGSTTPFATVTF
                     PDHCSGSLTGQEKVLLYMLFDLGACVGDEPNPPACSPQACPAYPSCGTRPDGCGDTLN
                     CGCNSGESCINGQCTKQGCVPTTCAAEGIICSTISNGCGQALQCPCDVCTPKTKQQAC
                     NGVTCGYASDGCSGVYLCSECPPSCKPLTACPPDKNCGVISDGCSGTLNCGTCTAGKV
                     CGGAGVPNRCDVPQCKPLTCRDLNASCGLIGDGCGSSVNCGACTAGQVCRTANGEQRC
                     DGCMPRTCESVAAECGLIGDGCGGTVDCGSCPAEQTCGANEPNRCGDGRKCVPLTCEA
                     AGAACGVIGDGCGGRLDCGTCPPGQICGLEKPFQCAAPPPCTPSTCEELGAECGFVAD
                     GCSDLLDCGECAEGFTCGLGQANECARIR"
     gene            complement(19690..20331)
                     /locus_tag="VFQ61_19925"
     CDS             complement(19690..20331)
                     /locus_tag="VFQ61_19925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956779.1"
                     /translation="MTNRSLSGAVFMFALLIGCGAAEDTPPSSPGNSGGRVGAGGKAS
                     GGTTSAGGTTSSVTSGGTAVVGGGGTSTSSSSAGIGLYSSVFEAGGTSAGGTSGAPPV
                     VCDDSEPGTCDWTRVEGCCKQWACNRANGEDVFNPRPITACEALVTCVRANPGCSTAS
                     DPLCFQDEKPTAPCLDEGYLASHEDPDGPFAWTEHLVRCVCGYPQEPASSEAE"
     gene            complement(20418..22949)
                     /locus_tag="VFQ61_19930"
     CDS             complement(20418..22949)
                     /locus_tag="VFQ61_19930"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HET9956780.1"
                     /translation="MTTHSNEEGPAGLSLSVNSARDQLNRGHGIGARTGRLGVIALAM
                     GALAACSNGTQPAGRGGLARGGTGPVIVDQSGSGGGGAVFTNGGSGPLIPNDGQGAKV
                     GSSGSGNTTGGGKKPTRVPLTYCLPEPFPKNYSCKGEEGSWAEALQKVLWFFNVNKSG
                     PGVYCTDVQWRGDAHVADGHIKLDPTDPNGVNLPQSFIDAHRSVLDPDGNNEVDLAGG
                     YHDAGDYIKFTLTTTYAASMIAWSLYEYPESFTTTSLAEEAMGQVRWASDYLMKATFR
                     DENGKLVAFAHQVSDVTDHSCFWMPPEVRRPDLCPRKAYFVWDEKPAADVTAAAAATL
                     ALTGLVTYQNRKTQADVDYAERCISYAKSLYEFASRYPEAREGDDNGLYLSNSSTDDL
                     AWAAIWLYLVNPSQNGAYLDAIVSGERPWLSFGAQANLLISGVTPGVWVPAWAESSPH
                     SWDAVRAGVIVKLAQIMAARKDPMATRWVDIARQMALGFKTGGSTPDGLYVYMSWGSA
                     RYNAAAQFTTLLFTKYFPDDPETPGLLTWANRQIDYILGDNNLGKSYVMGYTDSYPLQ
                     PHHAAGHASIYGLPAKPAENRHIIWGALVNGPKGDGTHVDARDDYGSNEVTIDYNGAL
                     VAALAAHYPNRGAGQCPLKAFPPLEKPIDEFYAMANLNMDLEGCRTQVSIRTMNESIH
                     PPRYDKSLTTRYWFNATEVIEAGLDPTQAITGQIVRDTGMSATPGDPTEMKGPIACDT
                     EAGVYYFEFDYTGSQFWGLIPVIGAPRDVLLDYGVAYNPGCPWNPENDWSIQYLKKGG
                     VDDVVKTPYVTVYSNGQLIWGKEPPCMGGGKEVEPPPPEIEYPPR"
     gene            complement(23368..24762)
                     /locus_tag="VFQ61_19935"
     CDS             complement(23368..24762)
                     /locus_tag="VFQ61_19935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9956781.1"
                     /translation="MRLTFLGAAQEVTGSLLLIEVSSGSLVIDCGMFQGRRQEATQRN
                     RELPREALAADLVILTHAHLDHAGSLPTLVKSGFTGSIYSTSATYDLCSYMLRDSARI
                     QASDAAYLNRKFADDPDWVAIEPLYGEQDVLTALQRFVALPYRRVFQPLPGVQATLIN
                     SGHILGSAQVILDVQEGAHTRRLVVSGDLGRKGLPIIRDPETPPRPVDYLVMESTYGN
                     REHGSAEQMHTDLERVIRETVARRGKVIVPAFAVGRTQELLFVLHELIAAGRIPHIPI
                     FIDSPLAIDVTQVFRSHPECFDAETRALLEARGDVFNFEGVRFMQSREESMQINQYAG
                     PAVIISASGMAEAGRILHHLRNHVEDERNTILIVGFMAQHTLGRRLVEGRQRVKIWGV
                     ERELRAHVEVLNAFSAHADRRDLLAYVEACTGLRRAFLVHGEPEQQGPLQAALNARGI
                     DVAIPHRGQTVTLD"
     gene            complement(24765..25442)
                     /locus_tag="VFQ61_19940"
     CDS             complement(24765..25442)
                     /locus_tag="VFQ61_19940"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9956782.1"
                     /translation="MSEHSARERDTTEHRTNGRCRIVLTGGPGGGKTTAADLFRREIG
                     EGVVVVPEAATLLFAGGFPRSSEFHAQRSVQRAIYHVQKNLEDVQSALYPERILLCDR
                     GTIDGAAYWPEAPTGFFDAVGSTLEAELARYDAVIFFESAAVGGISVEGGNPTRIESI
                     EQALALDRRLRDIWAQHPRFVIVPHNPSFMKKILFGLAALESIVAQLREPAAQLREPA
                     AQPRSRQ"
     gene            25792..25980
                     /locus_tag="VFQ61_19945"
     CDS             25792..25980
                     /locus_tag="VFQ61_19945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956783.1"
                     /translation="MKKVLLVAAVIAFCSMFQNSASAEPTGGTAAKTKTITIEPEQCV
                     TRCFFIMGVPVYCYEMCF"
     gene            complement(26203..27795)
                     /locus_tag="VFQ61_19950"
     CDS             complement(26203..27795)
                     /locus_tag="VFQ61_19950"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9956784.1"
                     /translation="MLSLRPYQQQAIDAVLAARRRGVRRLLVCLPTGAGKTVIFSRLA
                     QLAQRQVLVLAHREELLYQASEKLQHELGGQHLVAIERGSERGARDAKVLICSIRSLH
                     EARLAEVMRQRQLGLIIYDECHHAAAEDNLRVLRQLGAFDAKFPGTLLGFTATTARGD
                     GRGLDEIFEQIVYSRSLPELIQDGYLAPLRGFRISTAADLTRLSATGLDFREDELEEA
                     VDIEERNALVARSIQELARDRRTIVFAVTVRHARNLAYALNALGVATGIVHGDMPGEQ
                     RGRALSEFKAGKLQVMCNVAVLTEGFDDPGVSCIAMARPTRSEGLYAQCVGRGTRLHN
                     DKRDCLILDFVDASRLSLCSLPSLFGAPRELDLRGDDAERAARVWQQILVDHPGFELE
                     AGSITLPEIQQRAASFNPLTLETHDEVRAISANAWFSLGRHGVALHFERRPGVVCEVT
                     VLTRAARGKRWEVSLDGRVKERFSTLEAAVEAVDYEVSRFGPRAAASALASAPWRAAA
                     ADALRQAVWERIVGRKTATASD"
     gene            complement(27863..28276)
                     /locus_tag="VFQ61_19955"
     CDS             complement(27863..28276)
                     /locus_tag="VFQ61_19955"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET9956785.1"
                     /translation="MTSVKGSCLCGAIVFEMTAAPRGVTQCHCSRCRKTRGTGHAVNL
                     VVPLDGFRYLEGEELLTKYRLPEAKYFAHWFCSRCGSTLPRFDEGRGIGIIPMGSFDE
                     DPGVRPDRHIHVGSKAGWETIADDLPQFDAGPPAL"
     gene            28738..29904
                     /locus_tag="VFQ61_19960"
     CDS             28738..29904
                     /locus_tag="VFQ61_19960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9956786.1"
                     /translation="MSRTPPPVPLLLVLLCLCSTTVTCGREAPPANKAAPVQPALLGE
                     ESLATVKREDLDTGPLISGSLEPERRAEIVAEASGSVLKLQAELGDTVKRGQVLARIE
                     TTGIVDAYRSAKVAESSAQQALDLAQREFERSRSLVESGALPTADLDRARNAVEAAQA
                     QLADTQARTASTQRQVASAVATSPFDGVVSAQSVHEGDVVAPGTRLLTVIDPSSVRLE
                     AQVASSALPALSLGARVSFRVRGYPNQTFTGLIQRIAPAADPVTRQITLLVSIPNPQS
                     QLLSGLFAEGRVASEHRSALVIPTAAVERADQGASVIALRQGKAQRVEIELGLEDEAR
                     EIVEVKSGLNEGERVVTGAARKLTPGTPVRELPRPTTRAGTHSESELAQSQGAE"
BASE COUNT         5618 a         9752 c         8956 g         5650 t
ORIGIN      
        1 gcgcacggtt gtagaagcga gtcgtgacgt catcgagtgc gattggcgtc tgcccgacca
       61 tgtcgagcaa gcccaatgag ttgatcagcg ctgccgtctt tccgtcgttc gcgtcggcgc
      121 cgagcacgac cacgacgcca cccgccttcg cgaacgaatc gacgacgccc gtgctcgccc
      181 aagtggcgcc gagcccaatc agttcatcgc gcgttgcgtt cccttgttct tgaatcagga
      241 acacatcgta gttctgcacg ctgaggcggg ccgagagctg ggctgggtcg cgcatctcat
      301 cgacctgtgc ggtgcgaccg cgagcttcgc ccgaccaacg caccactttc tcgatttggc
      361 tcacggcgtt ggcgctcgcc caacgggaga atgagagcac gcggacgcga gcccgcaccg
      421 gtaagtacac cgcgttgccg agcaaggtgg tgtgtgccgt ctcgggctga gcccaactgt
      481 aattcatgca gtagagcgcc acatgaccgg ggctcgcgcc gacgcacatc cctgcctgac
      541 agatgcccga cgggcagagg ttgaagcatt ctccgcaatg cagcgggttc acgttcggat
      601 cgatgcattg atcaccgcac gccaagagcc cgggcggaca cgcggggaca cactcgaagc
      661 cgcccatttc tgcaggcgaa cagagcgggt tcggatcgac acagctcgtg tcgcaatctc
      721 cgcaatgctg ggaagtatcg aatggcgggt cgcagctcac acccgcagat cctgcttcgg
      781 gtcggggacg cgtgccgccg gttccgccta cgccgccgcg tgcgctggtt ccgcccgagt
      841 tggttccgcc actagagtca ccgcctactg ggtagggctg accggtgtag ccccccgtgt
      901 cgcccgcgcc gctcgcgccg ccttcaaggc tgccgttacc gcccgtgctc cggccaccgc
      961 tgttgtcgac tcctcccgta ctcgatgtgc attgcccatc gacgcaggag gcaccgtccg
     1021 aacacgtgtt attgcaagcg ccacagtgcc gagcgtccgt gctgggatct acgcattctg
     1081 agttgcagaa cagcatgccg cttcggcact tgccgcccac caacccgccg gtatcgcacg
     1141 cgccgagcac gccgagcggc aaggtcacgg cgagcatcag tcgacgaaac cacgcgttca
     1201 cggctcacct cgcgtattgg tgccgtctga tttgggcgtc ggcttagcgg tgggagccga
     1261 gccggatgca ttgttcgtgg tttcggaggg cggagcgagc acaggcgctt tgggcagcgc
     1321 gaggccaggc ggcttcgaat cccgacctgg gttctcgctc tggggctgcg tcgcacccga
     1381 gtcactcgaa ggcttcatct ctggttgttc cgattcggga ggcggaccgt cactgccgag
     1441 cgacggcggt ttcggaggca acggcgactt gccactcacc cgagcctcac gggtgatgat
     1501 gaactcgacc cgccgattct gataataggc ggactcaccc ttgctatcga cgaggggtcg
     1561 cttggagcca taccccgcat ggctcaggcg gttcttgtgc acaccgctct tgaccaagaa
     1621 ctcgagcacg ctgtaggcgc gtccacgact cagcttctcg ttgaactctt cggagccgcg
     1681 ctcgtcggca tggccttcga tcgagatgtg gatgtactcg gggtgatcca cgatcagctt
     1741 cgcaagccgc tccaagagct cgaagctctc tttgcggatg atgtggctgt tgatcatgaa
     1801 gtggacgcgg tcatcgagca cgatcttgtc gcccaccacg cgcacctggt cttcgtcggg
     1861 gcaaccgtcc gagtccgcgt agtcgttgat ggtctccggc tcgaaggggc acagatcttt
     1921 gacgtccggg atgccgtcct tgtcgttgtc cgtatcgggg cagccgtcct cgtcctcgaa
     1981 ctcgtctttg tcctcggcct cgttggggca acggtccttg gtgtcgagaa tgccgtcttt
     2041 gtcgttatcc acgtcggggc aaccgtcgtc gtcctcgaag ccgtcacggt cttccggttg
     2101 agccgggcaa tggtcggcca aatccaacac gccgtctcga tcgttgtcag cctcggggca
     2161 gccgtccgtg tcctcgaatc cgtctcgatc ttccgcatca tccgggcaac ggtcgagctc
     2221 gtccctgatt ccgtctttgt cccgatcaga atcgcgacgc agccccgaac ctgtatcgaa
     2281 gagagcgtgc acgccgacca agacgaggtt cgcatcttca ggccgcacct gattgttggg
     2341 ctgcatcaca tggtaaaagc ccaacatcgg ccccatgccg accttgcccg gagaatccag
     2401 aaagtcgtag ccaatctccg cgttgaacat gggccgcaac agctcgttgg tgtatgcggc
     2461 accgagcgtc gcgccgagcc agaagcccgc gggggaaatt cgagttccat cgtgcgaagc
     2521 ggcgaacgga cgcagccgcg gcccgacccc gcccgacaat ccagaaccct ctccttgcgg
     2581 ctcgatgtaa gggttctttg gcgggtcgcc ctgggtcagc cagatccccg caaactccgc
     2641 ctgcaggcca aaggcacgcg tgattggcag ctccacggag ggcatgaacg ccaaccccca
     2701 gccgtactcg cgttcttggt atccaccgag cgcacgtgtc gcggcggcag aaccatgtaa
     2761 tcgaggcggc tctgcgagag ccggccagct caccgaaatg agcccagcag caacaacggc
     2821 agaagtaaca aactgtccaa ggcgcatgga aggcggtccg ctctcggaaa cggctaatct
     2881 gtcccagcca taagtctgcc cggctcactc tgggatgtgt gtctgaatgt ctcgcagctc
     2941 ggtcgcaacg cacgcgcgag ccgccttcgc gaagaagcgt ccccctgttc ctctggcgtc
     3001 gtgctgcgct agagccgtgt cgcgctagag ccgcggaaaa atggcctaat tagcgtcgtt
     3061 catttggatg ctcgttgtaa aaagcatcca gactctcttt cgaaatgcgg actgaaacca
     3121 gaccgtctct ttgtaatcgg gcctggcagc cgagtcgcga ggacatgcga acgtcgaacg
     3181 ccttgtccaa aatgtcttct tcgccctctt cggccggcga aagtaaatcc gcacctgcct
     3241 ccacatagac gtggcaagtc gaacacgcac acacaccgcc acaggcgctg ccctcgggtg
     3301 cgcctgaccc ctgagcagcc gccaaaatcg agctcccgac cggaacctcg acttcgatgt
     3361 tttgatcgac gaagcgaacg cgcggcatca ggtgagcccc ttctcgcgta aatgagcctc
     3421 gacccccgcc gcgtgctgca cgcgctgttc cacttcctgc actgctttgc cggacacgga
     3481 gagcccgata gcacgattca tgcgacgtcc ggcgaattcg tgagtagcgt ccgccaatgc
     3541 gtcgatgcgc gcctgtacca gcgccccatc gctcgattgc acgatggctt ccttgagcgc
     3601 ggccacggcc gagtcgatcc tttcacgctc tgcgggcgcg agcagatcgg gatcctgttg
     3661 cagccctttt tgggtcgcca ggatcacccg ttctccttct acccgcgcat cggctaagcg
     3721 gcgcgcggcg aagtcttcct ctccgaagtc gagggcgtct tcgagcatct tctcgatctg
     3781 ctcgtcgctc agcccgtaac tcggcttgac ctcgacggtc tgcgattgcc ccgtggttcg
     3841 ctcactggcg gtgacgctga gcagcccgtt ctcgtcgacg tgaaatgtaa catcgaggcg
     3901 tgccgcgccc gcgggcatgg gcgggatgcc acgtagcgtg aagcgcgcga gggaccggca
     3961 gtcctttgcc aactcgcgtt caccctgaac gacgtgaatc tcgaagccag tctggttgtc
     4021 ggcgtaggtc gtgaacgtcg cgcgtgcacc cgccggaatg gtcgtatttc gaggcaggat
     4081 cttgtccacc ccgccaccca tcgtctcgat gccgagcgac agcgggagca catcgagcaa
     4141 cagcacgtcg tcgctcccgg tcaccaacat ctccgcctgg agcgcggcgc cgtacgccac
     4201 gaccaagtcc ggatcgatgt cggccagcgg gggcttgccg aacagctctt ggacgtagct
     4261 ccgcacgcgc ggaacgcgtg tagctccgcc caccaggatt acgccgtcca gttgctccgg
     4321 cgcaacctgg gcgtcgcgca acgctctgcg acaagctttc ccggtgcgct ccagcaaagg
     4381 ctggatgagc tcgtcgagct cgccgcgcgt cagcgtaaag atgcggctgc gattgctcgc
     4441 atccggcaac tcgacttcca ccgtttgctg ctcggtgagt gcctctttga cctctcgagc
     4501 cgaggccagc accacgctcc agagctcctt ggatgagcga gcacgcgagt gggcgtccaa
     4561 cgcggttaac atgcgatcag cgacggcgcg gtccatgtca tcgcccccga gagcgctgtc
     4621 tcccccggtg ctcagcacct ggaacacgcc atcctccaag cgaagcacgg tgacgtcgaa
     4681 ggtgcccccg cccaagtcgt acaccgcgaa caggccgttc tgttgcttgt cgaggccata
     4741 cgcaagcgcc gccgccgtcg gctcgttcag caatcgcagc acctcgagcc cggcgagacg
     4801 cccggcatct cgggtcgcct ggcgttgcgc gtcgtcgaaa taagcgggca ccgtgatcac
     4861 ggcaggcccg acgttacgca gctgatccac ggcgctctgc ttcaagctct tgaggatctc
     4921 cgcgctgacc tcgaccgggc tcgcttggag ttcgccgaga tcgaagcgca cgactttggc
     4981 ctcctcctcg gtctctggtc gcgcaaactt gtacgccgac aggcgctgcg tctcagcgtc
     5041 gttcgcgccg cggcccacga aacgcttgac gctctggatc gtccgttcgg gctcgcgcgt
     5101 cgcgtacgcc agtgccgcgc ggccaacgat cacattgcct tcgcgagcgt agtaaacgac
     5161 ggaaggcaac agcgccgtgc catcacaatt cgtgagcgcc agcggccgcc cgtcgcgcac
     5221 cgtcgcgacg atcgagttgg tcgtgcccag atcgattcca atcgccttgg gcggagcctt
     5281 ggggtcgaaa atgtctaata acaccatttc gaattactcg ttcgcgcgcc actagcggaa
     5341 gcctcgggcc tccgtcgagc ggtgcggcac ggcgttaact cgacactcag tcgaggtcat
     5401 ctagaatcgc gtgtgcctcg cccacgagtc gccgaaaaaa ccgcaactcg cccaaggttt
     5461 tcgcgattcg ttcgcgtgta gcgccaattg gtgggctgcc cccgtcggcc gtgttcgctt
     5521 cgagcgctcc cgcgctcaag cttctgaatg cctgaatcaa gcgctgttcg accgcagact
     5581 cgcgccctcg aacgttctca accaggcgct gaattgcggc aacgtcatgg cggcgctgag
     5641 cctgcgccag ctcttcccgg agctccagca cctccatcaa gaagtcaggt gaggcaacgg
     5701 cttccgattc cgaagcgacc atgccgtagc gctccaacag cgcctcggct cgccgcaccg
     5761 gatctttcaa gattcgcagc gcttcgttca cctcgatggc acgcgacaaa gcacgacgcc
     5821 gctcgacgct cgaccctcga acgtgccgat cggggtgcag ggccgagctc aactcacgat
     5881 gacgcgcttc ggcggcgcca aaatccaagt cgaaccgtgg ctctaccgcg aggagctcga
     5941 aagggtccat cgctactctc cttggctcgt tccttccggt cgacttggct cactccttca
     6001 cttcacagtg aaagagtgac cgcagccgca gctcgacgcc tcgttggggt tcttgaactt
     6061 gagcccttgg aacatcaggg tcttctcgta gtcgagcgta gctcccgcca agtaaatcag
     6121 gctcttcttg tcgatcacga acttgacgtc gccgaactcg aagacattgt cgcgttcccg
     6181 cggcgcctcg tcttcgaact ggatgacgta ggagaacccc gagcagccgc cccctcgaat
     6241 gccgagacgc accacggcgt ccggcgtccc tcgcgcatcc agcttcttac gggcaaaccg
     6301 cgccgcttcc tcggtgacga agatgcttcg cttcgagcga cccgacgcgg gagcctcact
     6361 cggcgcgcta gtcacggctg tggctccaac cggctgcgtc gagggagagg agggcaagct
     6421 aggggctgca ctagtcatgg cttctgttcc gagagggcct cgcgggcggc cctcttcttc
     6481 ttgtaatctt caatcgcgct cttgatggcg tcttccgaca gaaccgagca atggatcttc
     6541 accgggggca ggttcagctc ttcggcgagc tggctgttct tgatgctcgc ggcttcgtcc
     6601 accgtcttgc ccttgatcca ctcggtcgcc agcgaagacg atgcgatcgc cgaaccacaa
     6661 ccaaaggtct tgaacttggc gtcttcaatc acgccctctt cgttgacgcg gatctgaagg
     6721 cgcatcacgt caccgcacgc aggcgcgccg acaagaccgg ttccaacggt gggatcgtcc
     6781 ttgtccagca tgcccacgtt gcgtgggttc tcgtagtgtt cgatgacttt ttcgctgtat
     6841 gccatttcag actccagaaa atctctcgtt cggggcggct cggggtgggt cgccagcgcg
     6901 acctcagtgc gccacccatt ggatcgattt gatgtcgata ccgtcctggt gcatctccca
     6961 cagcggcgac atgtcgcgta gcttcttcac tttgctcacc accaagtccg ccacgtagtc
     7021 gacctcttcc tcggtattga aacgaccgag cccgaaacga atcgacgagt gcgccatgtc
     7081 ctcgtcgagc cccatcgagc gcaaaacata ggaaggctcg aggctcgcgc tggtgcatgc
     7141 ggagccgctc gacacggcca cgtctttgat ggccatcatc agcccctcac cctccacgaa
     7201 ggcgaacgag acgttgagat tgccgggcaa acgccgctcg gggtggccgt tcaggataat
     7261 ttgatcgagc tcgcgcgaga gcttgccgta caagcgatcg cgcaacgcgc gaagccgcgc
     7321 ggcctcgact tcgccctccg ccttgaggat ttcggccgcc ttggcaaagc ccacgatacc
     7381 cggcacattg agcgttcccg agcggttacc ccgctcgtgg ccgccgccat ccatctgcgc
     7441 taccaagcga acgcgcgggc ggctgcgtcg aacatagagc gcgcccacgc ccttgggccc
     7501 gtagatcttg tgcgcggtca gcgacgccaa atccacgttc atggcgcgca catcgaacgg
     7561 cgtcttcccg agtccctgca ccgcgtcgac gtggaacagc acgccccggg cgcgtgtgat
     7621 ctggccaatc tcctcgatcg gttggattgt tccgacctcg ttgttggcga gcatgacgct
     7681 caccaaaatc gtcttttcgg tgagcgcctt ttggatgtca cccggatcga cgatgccgtc
     7741 tttcccggca ttgacgtaag tgacttggaa gccctgcttt tccaggcgct tacacgaatc
     7801 cagcacggcc ttgtgctcga tcgtgctggt cacgatgtgg ttgcccttgc ccgagtagta
     7861 ctcggcaacg cccttgagcg cgagattgtt gctctcggtt gcgccggaag tgaacacgat
     7921 ttccttgccg gactcggcgt gaatcaagtc cgccagcgtc tcgcgagcgg cctctacggc
     7981 gccctcggcg tgccaaccaa aagcgtgcgt gcgactcgcc gcgttcccga actcctgtgt
     8041 gaagtacggc aacatggcct cgagcacccg gggatcgacc ggggtcgtcg catgattgtc
     8101 catgtagatc gggaacttca acgccatttt cgtacctcag ctcacgtgca cgcccgcgac
     8161 caactccggc acggcggggc gttcgggatg acgagcacac ttcgggcagg agtgaaccgg
     8221 cagctccgtt tcacacgccg tttggcacgt atccagatag gccagactgt ccgtcaattc
     8281 cgcttctagc gcgcggagct cggacagctt cgtccgcgtc tcttccaact tggcgcgata
     8341 cacctctcgc aaccgcgtcg cggcgaactg cgccgagcca gacgcttcct gctcacgcac
     8401 cagctcctgg atctcactca atgagaggcc caggttctgg agcttggtga tccagcgcac
     8461 ccgcaccaga gcctcttccg tgaagagccg atagcggccc ttcgaacgct cgtgtgcgcg
     8521 taggagcccg agctcctcgt acaagtggat ggcccgcacg gtcttgcccg cggccttcgc
     8581 cagctccccg acctgcagca actcctccga cgctgccgca cccaagccat ttccgtcggc
     8641 gctcggatct gcggcggtca catcgctacg ggggcgcgtc ggaaccccgg ccagaccaac
     8701 ggcctggggc agcaccacca atcggaggcg cttgtctgcg ggggtattcg aatccatcac
     8761 ggcaccgacg taggttcttg gagttcaggt ctgagtaggt tctgctcgga gaatagttaa
     8821 gtcgaggtgt cgcccaatga ggtttcgcga cggtctccct cgctgcgctt ctccggtgga
     8881 tgtcgcttat tgcctcgcaa agctcgtggc gatgtgggct ttagtacggc cttctcggtc
     8941 tgtcgaagcc taaccctaac gcgcgggtaa gggttacgct acggaagcta cttaggcccg
     9001 ccaacccaca tctgtcaacc caattaccga aaattccgtc gccaacccga cggacgacat
     9061 ccactgaggc agggcgcaac aattcgctgg actcggcggc agtgatgcga ggacgccgcc
     9121 gcttgggttt ccgaaacaca cgctagagtt ctgggatgcc caatccgctt cgtcggagcg
     9181 cgctctgctt cggaatagtg gtcagtgttt ccgtgcttgg cgcggctcga ccgacgggca
     9241 gcgcggctag gccgacgaaa gcgcccaaaa cgccggttgg gacgggggcg cccaggcgct
     9301 tgagctacga agaggctcag cttttcgttt tgtcgctcat caatcgtgac cgagccaaag
     9361 ccaagctccg ccccgtccag atggatgatg ccgcctccag ggcggggcag aggcacgcgc
     9421 gtgatctggc gacgcacgga ttcacgggtc acgtgggcag tgatgggtca gtgccggagc
     9481 agcgatacac ggaaagtggc ggacagcact tcgtacagga aaatgccgcc tgcatctcgg
     9541 acacgacgct gcgtcagctc gcgccgaatg cgctttacga ttcgtccaag cttgcggcac
     9601 tgcaccagat gttcatgtcc gaaatgccac ccaacgacgg gcatcggcgc aatattctga
     9661 acccctcgca taactacgtc ggaatcgggc tcgcactacc ggcagacctg ccgcagccgt
     9721 gcctaacgca agagttcgtg gaccagtatg gtcactacga ggcactaccg agcgccgcaa
     9781 agcgcagcgc gaagtttcgc gtcgcgggaa cggtttcgga gcctctcctg ttcgggggcg
     9841 tcggtctcgg caaaacgccg ctccccgttc cgccgcccaa acgacatctg aacggcaatc
     9901 tgtaccggat cccagctccc gataagatgt tcttcccagc cggcttcaag acccccaagc
     9961 ccgtgagcgt cgatggccca cgcttcgata tcaacttgga tttggggaaa aatgcagggc
    10021 caggcctcta cgcgatcagc atctgggcga agcagccggg gaccactcgg ctcttcatga
    10081 tcagtatgcg cacgatacgc gtggaataac ctccgggcag aaaagcgtga tgccctgcgg
    10141 cccgattctg tattggcgcc gtgaatcagt ggcgcgtcgg cacctgcaga tttatgtgtg
    10201 gcaaggaact cactcgaagc ttcgactttc gtgagcgtca catctaaagt gttggtagga
    10261 tgcgggtcat gggaagcggc gtccggagat cgctgtggtg gctgagcctt atttcgacgg
    10321 cttgttgggg gcgtttagag cacgagaggt cagatcaggg cgccagtatg cagtcttcgg
    10381 gtggcgtcgc cagcggcgga ttaacgtcca cctcttccgc cgagaactct tcgggaagtc
    10441 ccattggtga tacgccggag agctctggag ggaactccgg ccccccttca tctgtttgct
    10501 tcgtcccaga atccgtttcc caagataccg actctaagtt cgtcgatccc ctcgaggtgc
    10561 gcgatcggat cacgcggctt cttctaggag tgccgtcatc ggcctatgcg ttacctgaca
    10621 cgactacgcg cgcttgggcc gctgagctcg cacgacagat cgcgcgagac acggtgccat
    10681 tgcccttcct acggcgattc ttcgccttct ggttcgacaa cgacgaggcc tggaacacgc
    10741 agaagcccta ccaagccacg tcactagcgc tcgagtgggg cgacacttgg gcgacgacgc
    10801 tcgcccagca ttggagcttt cagaagctaa tgaccgcgag cacgccccaa gcgcgggacg
    10861 tcggcctcct caccgacacc cgtttgctta gtcggtatac ggaaatcgcg aaccgcgggg
    10921 cctggctgac caacaaactt ctttgcctcg gcgcgccgcc ggaaccaacg aatttccgtg
    10981 cgccccctcc cccggaagga attacgcggc gggaccattt ggcggcacag gtcgatcatg
    11041 aaccttgtcg gagctgtcac cattggttcg atcccgcggg ctgggcgctc gagcatttcg
    11101 atgaacgggg agagtaccgc acatccgatc ggggtctttc catcgatagc gccggtgaac
    11161 tcccgttggc cttcgacgac agcaaggtca cattttccag tatcaatgat ctgagcgagc
    11221 aactcgtggg ctcctgcgcg gtggctcatt gcatggccaa aaccctcacg agtatggcgc
    11281 tgaatgcggc cggcgtctcc gcaaccccga gcgaagcgga gcttcaacac atcgccaatg
    11341 agttcaggag tgagcattcc gcgctggaca tgattgcagc tatcgcggcg agcccgagca
    11401 tgcttcggtg agcttggatt tcgcaccacc tccaggctca cgggcattcg ccgttcacgc
    11461 ctgttcaccc gaccgcagcg accacaccga gagttcttgg ctgatcttct cgaggtaacc
    11521 tgcttctctc acgacttctc gatagcccgg caccgcgcca acccactctc ccattcgagg
    11581 attcggagcc tgcgtcaggt atagggtgcg aaggcccatt agcgcgggcc cgtccattcc
    11641 cgccgtggtg actccaacct gcccaagcag gccgtgccgc tgtatcagat actcgaagaa
    11701 ctggagttgc gagcgccgca tatccacagt tcggaagatg gggtctttcc aaaagagaat
    11761 catgtcgatg acaccgtcgg gaatcgaccc gtcccgcaac gcatcaccaa tccaaatggg
    11821 agtcagcccg gcgccttgca cgcgtcgcgt cagttccagt agctccaagt attccgtgtt
    11881 ccggccaggg tgatgcgcgc cgtctcgaat ccagagcagt accttcttct gctgcatgcg
    11941 agacacggat gctgtttgga cacggtcttc gatccaatca gcaacggcgc cagagatcgg
    12001 gccatttccc agaaaggcag atcgcagacg tcgttgcgcc gtgatcgcgt ccgctcgaaa
    12061 cgcacgcgcg atgctctccg tcgtgtgctt catggcgcac ggccgcgcgc ggtacgggcg
    12121 aatttgactc gccaggccgg cgtccaacag gaacttctcg gctgttggag tcccgtcttg
    12181 cccgggctcg atctgaatca gtaccccctc gagcacggcg gccgcggcga gataccagcc
    12241 ctccccgaac gagaaaccgc tttgggtgtg aaaagtgtaa gaccgattgc gatccgcgat
    12301 cacctttcta gctgctttgg cggcggcctc cgcgctacgc gccgcccggc tcgcagcggc
    12361 tacggcgtag ccttcgtgct ctagttggac aacgatttcg gcggagtccc gtgccgccct
    12421 ctcggcgatc cggacaatcg cctcgacctt ttgccaaccg gcttcacagg cttcgcgcgt
    12481 tggcgcgcgc tgcgcgctca aggagagctg attcgctccg tgacaggcat cttcagctcg
    12541 cgcggcatag gccctgcaca gggtcgaacc aagccggggc gccaccgcgg cgacctgccg
    12601 ctcattcgag tggtgacgtc caagaccacg ctcagtctga ggctcccgca gtgtcgtgac
    12661 ctcgagcggg gccgcaatat cgagtagagc cagctcgttc tgatagaccg cccaagcttg
    12721 ctctgcttgt tcgatgatgg tcgcgatttc ggcacgaaag cgtgccctaa ctccgggttc
    12781 atgctgggca ctgaggttgc gcagcgccgc ggtgagccga gctgctgcag ctcgctcgag
    12841 cagctgcgag gccagcttgg gcgctccagg caccaccggc gacgcttgct ccacgtcaac
    12901 ggacacttcg agactcacgg cgagcgggca cagcgcaacc ctaccgcgta atcaccccga
    12961 taggtatcgg cgttgtacga gatttggctc cgattccagg agtacatgag ggtggccccc
    13021 aggctggccc cggtgtggaa cccggcgccc gggagaacgc gattcgcggt gttccccgta
    13081 aggtacgcgc aatcctggca tggagaccca tacttgtccg cataacgatc gacgagccac
    13141 tcccacacgc tgcccacgag gtcgagttgc ccgtagcgcc cggctccctt cttcgcaaag
    13201 ccaaccgggg caacgttgac caaaccagtg cacgtgtcgc gaccactagc gccactgcat
    13261 tgcccggacg gatagcaaca atcccagatt gcgtagtcgt tgctagcccc cggcgcggtg
    13321 gatccccacg ggtaagcgcg ctgctcgtcg cctcccgcag ctgcgaactt ccattcggct
    13381 tcgctcggta aaaagccacc atcccaaata cagaacgcgt aagactcgta ccaactggta
    13441 cacgtaatcg gcaacgaatc gttcttgccg gactcgtcgg accaagatcc gtagatgttg
    13501 cacccactgt tggcgttggt acccttggcc gtgagcagcg ctttccactt ggcggtcgcg
    13561 cccgcgccac tcgggatctt gctgctccaa ctagcgtccc agcccgcctc gaacgcgccg
    13621 gacttaccgc tgtcggccaa tcccttgccg ccgttcaaat gcgcgtgttt gccgctacct
    13681 gccgcgggcg gggaaccacc gttctccagg tatttcacgt attcccggaa gcgagcgacg
    13741 gtcacttcat acttgtcgag ccggaatgag ctgatggtgg cggcatcggc tttctctgtc
    13801 gcaccgctgc ccgtgttctt gtaggtccgg gaataggtgc caccctctac cgtagggctc
    13861 gagcagcaac tctcatcgcc cttctcaccg caggtcgtcc ttccggggcc cgccgtggcg
    13921 cagctgggtg gacccatgct cgcgccgccc gatgctccac cagttgcgcc ggtggagttg
    13981 ccgcctgcgc cgctccctgc cgtgtcgctg cctccattgg cggcgcgacc gccgtcaccc
    14041 gggataccgc ctctacccgc ctggttgatc ccgccgctcg ctcccgctcc cgcgctccct
    14101 gattgcccgc cactctgagc ttggccgccg acgccactcg cgcctccgct cggggaacgc
    14161 ccaccactac cggtaacgct ttgacccgcc gcgccggtca tcgcgacgcc gccagtggtg
    14221 ctgccaccgc ctgtgctgtt agcgctcgct ccgccgttcc ctgccgtcgc gctcgcgccg
    14281 cttcctgacg tgttgccgtc gcccatcgcc ccgccggtga tcgagttggt attgccgctg
    14341 acagaaggtc cttgcgggcc atcactgttt ccacacccag ccgccgatac gatgcaaact
    14401 ccgacaccga gaatggccat cagccgcgtc tgcccgcggg catgtcgccc aatcgaacgt
    14461 cgaccatggt aagagtgaac tttgctctcc tcgattacat cgcagtcgcg gaacatggct
    14521 ccgaaagctg catgatagcg gcttggcctg caagaggctg attcggaatt ctcgcgctcg
    14581 agcaaacgcc gcagcacgcg agccaaaaca cgtcgatcga aaatcggctc aacaaacatc
    14641 acggctcaac aaaccgagca actccagcgc ctaaacagga tgcttcaccc gctgccgaat
    14701 tctccaatcg ctcaaaaacg ccacgcgagg ttccgagctg caagcgaaag ctcgcttcgg
    14761 aagaccgaag acaaacggcg ccgcatttcg aataggagcc gtgaagcagg agaagccaca
    14821 ttgaacgggc gaagcggctc gacatgagct tcccaagcag gcccgaaccc gatattgccg
    14881 ccatcgacgg cggctgaaca gtgaatctcg acctgtacca ccgagttcta acatcgaatc
    14941 aaagcaccag acatcgattc tccgtgaggc ctcaccccat ttcgattcgc gctttccaag
    15001 aaagtgggtt ggctctagag aaagcgcgca agccaggttg tgactcagct aatgcccccc
    15061 aatgctccgt cagccctcct gaagtgagcc ggcgcgcgac gcggccggcc cggttggccc
    15121 ggagtgggta agggcccgag cccgcccgtc cagccacact tcacacccag ctcgagtcaa
    15181 gtcgagcaac ccgggcagag cactctgaac cactcaacag ctacgcaagg cagtgccgat
    15241 acatgtaacc agaaatcccc ttccgagcgg cgacgaaaga ccggcgagaa caggaccaag
    15301 ctagggacca actggcgcgc aatgagtgcc ccgagagaaa caccatcgaa catctccgtc
    15361 cacaggccga tccagacagg ggctctcgcc atccacggtc atttaccgca acgcgcacaa
    15421 tcggtgtcga taatcgcaca tggtacacgt caatcaccgc gcgatcacac gctttcatgt
    15481 tgagccgtta tgtcccgcta tcgccactca gtcgcagcgc tcaattgcca ggaatcacat
    15541 gccaggaatc gcatgccagg aatcacatgc caggaattac actggcgggc cccgcgaacc
    15601 ctgcttggaa gctacgcagt catcatcctg agtgattgac gcccacgcgt tgacgagaaa
    15661 acgcggatgg cgtagagatt gaagttctag ccggagcaac tcgtgtacta cttctattcg
    15721 aggcggttgg cgtgtccgtg ggctttagcg tcgctactgg tggcgagcct attggcagct
    15781 tgtggcggcg acccagaggc accaaacacc ggggacccct atgccaatgg tgctcggggc
    15841 ggcacggccg gcacagactc gggcggtacc tcgaatcgag gaggcaccgg tggggtcatc
    15901 aatataggag gcggaaacgc ctgcgttcct catacctgtg aatccctcgg gacaagctgc
    15961 ggcacagtcg ctgacggatg cggagagatc ctcaattgcg gggaatgtga agcagatggg
    16021 ttatgtggcc tgcttagtcc aaacgtttgc gccacaccgg atgacctttc aacactgtgt
    16081 cagccactct cgaagaagga agcctgtgcc aacaagcagt gtggaatcga aggagacggc
    16141 tgcgggaatg tctacgagtg tggagactgc ccgcagggcg aagcgtgcgg attggatgaa
    16201 gcgtttcaat gtgctccggc catcacggat cccacggaaa actgtccagc gcgaatcgag
    16261 agctgcgagg ccgctgacgt cgaatgtggc gtgattggca acggctgcgg cggcatcatc
    16321 gactgtgacg ccgagaaggg cgggtgcgca gcaggccaag gttgcggagt ggcagagcct
    16381 ggaaagtgtg gggagctacc cacgtgcgaa ccattcacgg ccgcggaggc gtgcgctgga
    16441 aagtgcggcc tagtgtccaa cggatgcggc aacgatgtgg atggcggcct catagactgt
    16501 tcggcgatgt tcccgtgccc caatggccaa acttgcggcg ggggaggcgt cccgaaccaa
    16561 tgtgggacga gcgccaattc gtgccagccc gtagccaaga ctcaggcctg cgaaggtatc
    16621 gagtgtggcg cggttgggga cggctgtacg ggcagctatt cctgcgggac gtgctcggga
    16681 ggcgccttgt gcgtgcaagg ccagtgcgag caaccgagtt gcagcgcgat tccgcgtgcc
    16741 acggcgtgcg ccgggaaagc ttgcggcgcc gtgggggatg gctgcggcgg cacttacaac
    16801 tgtgggacct gcggggcgaa ccaacagtgc ggcctgcgta cggccttcca gtgcggcacg
    16861 gttactccga cgagctgcac gccgctaacc aagcagcaag cttgcgcggg caaagagtgc
    16921 ggcaccgttt acgacggctg tgggagcgct ccagccaatc aaatcaactg cggaacttgc
    16981 accacggggc agttctgcgg acttcgacaa gcctttcaat gcgacgcgcc ccaagttcct
    17041 acctgcacgc ccaatgccac gagttgcgcg gcgttgggtt gggagtgcgg catggcagtg
    17101 aacagctgcg gaactgtcta tgactgtgca actgagggcc gcagctgcgg tccgctggag
    17161 acctgtgtcg gcgggatcac tggcccagcg cgttgcgaaa ccccggctgg gttgaattgc
    17221 cccctctgtg cggcggtcgc ggactgtagc gggcgccctc aacgcacgag gttggcgggg
    17281 cgggtcgtaa cccccggccg cgccaacaat gatactggca atcaggtggg cgtgccgaat
    17341 gcctacgttt acattctgcg caccaacaac gtcgcagatt tgcccgcggt cggttccggg
    17401 atcccggccg gcggcgagag ctgcgaccgt tgcaatgacc aggacttggg tgccgtgctg
    17461 gccagcagcg tgaccagcgc gaccggtgag ttcgtcatcg aaggcaacgt tcccgtcggt
    17521 cagcagttct tgctcgtggt gaaggttggc aagttccgaa gggctgtgca gtacacgctg
    17581 cccgccagtg ccgcctgtac caccactaac ttgcccaccg ccgtcgcgac caaccccact
    17641 cggctgcctc gcaccatgag cgacggcttg gccgtcaaca ttccccgtat cgcgatcacg
    17701 acgggtcaga tcgacgcgat ggagtgtgta ttcgagaaga tggggatcgc gagcacggaa
    17761 ttcaccaatg gctctgcgac gaccagtggc ccccgcgttc acttgtaccg gggtgggtcg
    17821 tcgtccacac cgagaggcgc cagcctgaac gcgagcaccc cccatgacac cgcactgtat
    17881 tcagatgcgg cgcgcatgcg gagctacgac atggtcgtcg cggattgcga aggccagacc
    17941 tgggacagca gcttctctca acgagacagc tacggtggca atgtccgcga ctacgtcaac
    18001 cgcggtggcc gcctgttcgc gagtcatttg agcttcagct ggttgcacga aaacggcacg
    18061 gccgcttacg cgaccggctc agcgcgtttt gctaccggct tgggcccggc ggcaacctgg
    18121 gatccggcgc tctataccga cacgagcggc ctcggaaggg tcgctatcgg tcgaccgcgc
    18181 gccagctcgc ggatccagaa cttcgccgac tggatgatca accaaggtgt aattgcgaat
    18241 tctaccgcca ccttcacgat caccgatcct cgaagtaacg cgaccggcct cggccccgcg
    18301 agcgaagagt tcgtatatcg aaccaacggc aaccaacgaa ctcaacagtt ctccttcaat
    18361 acaccgtatt cggctccgca agcggcagct tgcgggcgcg tggcctacag cgggttccac
    18421 gtcgcgagca cgggcggcag tactactccc ttcgcgacgg tcacctttcc cgatcactgt
    18481 tcggggagtc tgacaggtca agagaaggtg ctgctctaca tgctgttcga cttgggcgcc
    18541 tgcgtggggg atgaaccgaa tcccccggcg tgctcgcccc aagcgtgccc tgcgtacccg
    18601 agctgcggta ctcgaccgga cggctgcggc gacaccctga attgcggctg caacagcggc
    18661 gaaagctgca tcaatggtca gtgcacgaag caaggttgtg tgccgacgac atgcgccgca
    18721 gaaggcatca tctgctccac catctcgaat gggtgcgggc aagcgctaca gtgcccctgc
    18781 gacgtgtgca cacccaagac caagcaacaa gcttgtaacg gtgtcacgtg cggctacgct
    18841 tctgatggct gcagtggagt gtacttgtgc agtgagtgcc cgccgagctg caaaccgctc
    18901 accgcttgtc ctccggacaa gaattgcggg gtcatcagtg atggctgcag tggcacactg
    18961 aactgcggca cctgtacggc cggtaaagtc tgcggtggcg ccggtgtccc caatcgctgc
    19021 gacgttccgc agtgcaagcc gctcacttgt agggacttga acgcgagctg cggactgatc
    19081 ggcgatggct gcggcagctc cgtgaattgc ggcgcttgca ccgccggtca ggtctgtcgc
    19141 accgcgaacg gggaacaacg ctgcgacgga tgcatgcccc gtacttgcga gtcggtggct
    19201 gcggaatgcg gtctgatcgg cgatggctgc ggcggtaccg tggactgcgg ctcttgcccc
    19261 gcggagcaaa cgtgtggcgc gaacgagccg aaccgctgtg gtgatggtcg caagtgtgtt
    19321 cctctcacct gtgaagcagc gggcgcggcc tgcggcgtca tcggagacgg ctgcggcggg
    19381 cggctcgatt gcggcacatg tccacccggt cagatctgtg gattggagaa accgttccaa
    19441 tgcgccgcac ccccgccctg cacgccgagc acctgcgagg agctcggcgc agagtgtggc
    19501 ttcgttgccg atggctgcag tgacttgctc gactgtggtg aatgcgccga gggcttcacg
    19561 tgcggactcg gccaagccaa cgaatgcgca cgcattcgtt gaggcctgtc tcgatcgagc
    19621 tcagctcatc ggtaaaccgc aaacccgagg ctcactctcg cgtaagtgag cctcggtgaa
    19681 gctccgctct tactcggcct cgctcgacgc tggttcctgc gggtaaccac agacacaccg
    19741 aacgaggtgc tccgtccacg cgaacgggcc atcgggatct tcgtgggagg cgagataacc
    19801 ttcgtccaag cacggtgcag tgggcttctc gtcttgaaag cacagtgggt cgctggcggt
    19861 agagcaaccc ggattggctc taacacacgt caccaaagcc tcgcacgcag tgatcggccg
    19921 aggattgaac acgtcttcgc cgttggctcg gttacacgcc cactgcttgc aacaaccctc
    19981 cacgcgggtc caatcacagg tgccgggctc ggagtcgtcg cacaccaccg gtggggctcc
    20041 cgacgtgcct cctgcgctcg taccacccgc ttcgaatacg gaactgtaca agccgattcc
    20101 ggccgagctg ctgctcgtgg aagtgccgcc tccacccacc acggcagtgc cgccgctggt
    20161 gacagagctc gtcgtcccac ccgcgctggt ggttcctccg ctcgctttgc cgccagcgcc
    20221 gactcggcca ccagagttac cgggagacga cggcggcgta tcctctgcgg caccgcaacc
    20281 gatcagcagc gcgaacatga aaacagcacc ggataaggat cgattcgtca tgtgtagttg
    20341 ctcgtaaaag gcacccgtcc tcgagcgccg cactagcatc ggtatgagta ttcctgagta
    20401 gcactcgaag cggacgctca gcgcggcgga tactcgatct cgggtggtgg aggctccact
    20461 tctttgccgc cgcccataca aggcggttcc ttgccccaga ttagctgccc gttgctgtac
    20521 acggtcacgt acggcgtctt gaccacgtca tccacgccgc ccttcttgag atactggatg
    20581 ctccagtcgt tctccgggtt ccaggggcaa ccgggattat aagccactcc gtaatccaag
    20641 agcacgtcgc gcggagcgcc aatcaccgga atcaggcccc agaactgcga gcccgtgtag
    20701 tcgaattcga agtagtaaac gcccgcctcg gtgtcacagg cgatcggccc cttcatctcg
    20761 gtgggatctc cgggagtcgc gctcatgccc gtgtcacgaa cgatttggcc ggtaatcgcc
    20821 tgcgtcgggt cgagaccagc ctcgattacc tccgtcgcgt tgaaccaata tcgcgtggta
    20881 agcgacttgt cgtagcgcgg cggatggatc gactcgttca tcgttcgaat gctgacttga
    20941 gtgcggcaac cctccagatc catatttagg ttcgccatcg cgtagaattc gtcgatcggc
    21001 ttctctagcg gagggaatgc cttcaacgga cattgccctg ctccccgatt ggggtaatgc
    21061 gccgcgagcg cagccaccag cgctccgttg tagtcgatcg tcacctcgtt cgacccgtag
    21121 tcgtcgcgcg catcgacgtg ggttccgtcg cccttcggac cgttgaccaa cgcgccccag
    21181 atgatgtgcc gattctcggc cggcttcgcc ggcagtccgt agatggacgc atgtccggcc
    21241 gcgtgatgcg gttgcaatgg gtaactgtcc gtataaccca tcacgtaaga cttacccagg
    21301 ttgttgtcgc ctagaatgta gtcaatctgg cggttggccc aggtgagcag gccaggcgtc
    21361 tctgggtcgt ccgggaagta cttggtgaag agcagcgtcg tgaactgggc cgcggcgttg
    21421 taccgagccg agccccagct catgtagacg tacaacccgt ctggcgtcga accaccggtc
    21481 ttgaacccga gcgccatttg tcgcgcgata tcgacccagc gcgtagccat tgggtctttg
    21541 cgagcggcca tgatctgagc cagcttgacg attacgcccg ctcgaaccgc gtcccaagaa
    21601 tgaggtgagc tctccgccca agccggcacc cagacgcccg gggtgacccc gctgatcagc
    21661 aggttggcct gagcaccgaa cgacagccag ggccgctccc cgctgacgat ggcgtctaga
    21721 taggcgccgt tctgactcgg attcaccagg tacagccaaa tggcggccca cgccaaatca
    21781 tccgtggagc tattcgacag gtacaggccg ttgtcgtcac cctcacgcgc ttccggataa
    21841 cgagaagcga actcgtacaa ggacttggcg taggaaatac agcgctccgc gtagtcgacg
    21901 tccgcttgcg tcttgcggtt ctgataggtg acgagcccgg tcagggcgag cgtggcggcg
    21961 gcggccgcgg tcacatcggc ggccggtttc tcgtcccaaa cgaagtacgc tttgcgcggg
    22021 cacaagtcgg ggcgtcgcac ttcgggtggc atccaaaaac acgagtgatc ggttacgtcc
    22081 gagacctgat gagcaaacgc caccaacttg ccgttctcat cgcggaaggt ggccttcatc
    22141 aagtagtcgc tggcccaacg aacttggccc atggcctctt ctgcgaggct agtcgtcgta
    22201 aacgactcgg ggtactcgta caggctccag gcgatcatcg aggctgcgta agtcgtcgtg
    22261 agtgtaaact tgatgtaatc gccggcgtcg tgatagccac cggccaaatc cacctcgttg
    22321 ttgccgtcgg gatcgagcac gctccgatga gcatcgatga aggactgcgg caggttcacc
    22381 ccgttcggat cggtgggatc gagcttgatg tgaccatctg caacgtgcgc gtccccgcgc
    22441 cattgcacat cggtacaata gacgcctggc ccagacttgt tgacgttgaa gaaccagagc
    22501 accttctgca gcgcttcggc ccacgacccc tcttcaccct tgcaactgta gttcttggga
    22561 aagggttcgg gcagacaata cgtgagtggg acgcgcgtgg gcttcttgcc tccgcccgtg
    22621 gtgtttcccg atccggaact accaaccttg gccccctgtc cgtcgttcgg gatcagcgga
    22681 ccactcccgc cattcgtaaa caccgctccg ccgccaccgc tacccgattg gtcaacgatg
    22741 accggaccgg ttccgccccg agccaatcca cctcggcccg ccggctgcgt gccgttcgag
    22801 caggccgcca gtgcgcccat tgcgagcgcg atgaccccga gccgaccggt gcgtgcaccg
    22861 ataccatgcc cgcgattcag ctggtcacga gcagaattca cggagagaga gagtccggcc
    22921 gggccttcct cgttcgaatg cgtagtcacg atgctcgatc ctccaggaac cgaatgaata
    22981 agcgtagcgt gcaaggacag tgcttcgagc ggtgttgccc aggtcacagg cccattcgtc
    23041 gatgatcgca gtcatggact gatgggtgtt gccttcaaca ggcgggcgct tgaatcacac
    23101 cccacggtta ccacgcgccc gccacagaca ctcatggtcc ctttgctcga gctgcgcacc
    23161 gacaggtgac gtgctgagcg acgtcgcctg gccatccatc ttgcgtcggg atcgcgcaaa
    23221 gagcgcgcga ctcgcggccg ttacgcgcag atttcgcagc tcatcccccg cttccgactc
    23281 gagtgcgaac gcttcagtta cgtgagcgcg agtgccccgg ccaggcgacg ctacgtcacg
    23341 agtcgcctcc ggcccgtgag cttcagctca atcgagcgtc accgtttgac cacgatgtgg
    23401 aatcgcgacg tcgattccac gcgcgttcag cgccgcctgc aacggccctt gttgctccgg
    23461 ctcaccatgg acgaggaacg ctcgacggag accagtgcag gcctcgacgt acgcgagcaa
    23521 gtcccgtcgg tcggcgtggg cgctgaaggc attcaggacc tcgacgtgag ctcgcaactc
    23581 gcgctctacc ccccagatct tcacccgctg cctcccctcg accaagcgcc gccccaaagt
    23641 atgttgggcc atgaatccga cgatcaggat cgtgttgcgc tcgtcctcca cgtgattgcg
    23701 caaatggtgg aggatgcgcc ccgcctccgc catccccgac gcagaaatga tcacggccgg
    23761 gcccgcatac tgattgatct gcatcgactc ctcgcgcgat tgcatgaatc tcacgccctc
    23821 gaagttgaag acatctcctc gtgcctcgag cagcgcgcgg gtctccgcgt cgaagcactc
    23881 gggatgagag cgaaaaacct gggtcacgtc gatggccaat ggggaatcga tgaagatggg
    23941 aatgtgggga atgcggccgg ccgcgatcaa ctcatgaaga acgaacaaga gctcttgtgt
    24001 tcgccccacc gcaaaggccg gaacgatcac cttgcctcgc cgcgcgactg tctcgcggat
    24061 cacgcgctcc agatccgtat gcatctgctc tgcactgccg tgctctcggt tgccgtaagt
    24121 gctctccatc accaagtagt ccacgggccg gggcggcgtc tcgggatccc gaatgatcgg
    24181 cagaccctta cgcccgagat cgcccgaaac caccaaccgt cgagtgtgag ccccctcttg
    24241 aacgtccaag atgacctgcg ccgagcccaa gatgtggccc gagttgatca gggtcgcttg
    24301 cacgcccggc aacggctgga acacccgacg atagggcagc gcaacgaagc gctgcaacgc
    24361 ggtgagaaca tcctgttcgc cgtacagcgg ctctatcgct acccaatctg ggtcatcggc
    24421 gaacttgcga ttcagatagg cggcgtcaga ggcctgaatg cgagccgagt cgcgcagcat
    24481 gtacgagcac agatcgtacg tggccgaggt agagtaaatc gacccagtga agccggactt
    24541 cacgagcgta ggcaggctgc ccgcatgatc gaggtgagcg tgcgtgagaa tgacgaggtc
    24601 cgcggccaat gcctcgcgcg ggagctcacg gtttctctgt gtggcctctt gtcggcgacc
    24661 ttggaacatg ccgcagtcga tgaccaagct gccactggag acctctatga gtagcagaga
    24721 gccggtgact tcctgcgctg ctccgagaaa ggtcaggcgc atgctcattg tctagacctg
    24781 ggctgcgcgg ctggttctct gagctgcgct gctggttctc tgagctgcgc tactatgctc
    24841 tccaaagccg ccaggccgaa taggatcttc ttcatgaagg aggggttgtg aggcacgatg
    24901 acgaagcgcg ggtgctgcgc ccagatatcg cgcaggcgac gatcgagagc caaagcttgc
    24961 tcgattgact cgatccgcgt ggggtttccc ccttccaccg aaatgccgcc gacggctgcg
    25021 ctttcgaaga agatgaccgc gtcgtaccga gccagctcag cctcgagcgt actaccaacg
    25081 gcatcgaaaa agccggtggg tgcttcgggc cagtaagcgg cgccgtcaat cgttcctcga
    25141 tcacagagca ggatgcgctc cggatagagc gccgactgca cgtcttccag gtttttttgc
    25201 acatggtaga tggcccgctg caccgagcgc tgcgcgtgga actctgagga gcggggaaaa
    25261 cctcccgcga aaagtaatgt tgcggcctcg ggcacgacca ccaccccttc cccgatctca
    25321 cgccggaaca ggtcagcggc ggtcgtcttg ccaccacccg gcccgcccgt gaggacgatt
    25381 cgacaacgac cgttcgtgcg gtgttccgtc gtgtcgcgtt cccgcgcgct gtgttcgctc
    25441 atcctcgatt caccttcatt gcttcgaatt tgcctccgcg gatagcactc acttgtatta
    25501 agacactcac acgcctcgga gctagtcgtt tacggtgagg ccggccgctg ttggcccaga
    25561 gcatgaccgc gatcgcagtc catatagttt ttacggggcg gcgggtctgc gcacgcatgt
    25621 tacagccccg cagcaagtgt gcagacctaa gcagctcggc tcgcctctaa tcccgtaatg
    25681 gagcgcggcg cgcctgctac cgtgaatgac cattgtggag gagggcttgg atccgcttca
    25741 atcggactca tcgggcgtgt gtggagggcc tccattagga aggtcattca gatgaagaag
    25801 gtgcttctcg tcgccgcagt catcgcgttc tgctccatgt tccaaaactc tgcgtcggca
    25861 gagcccaccg gcgggaccgc ggcaaagacc aaaacgatca ccatcgaacc ggaacagtgt
    25921 gtgactcgct gtttcttcat catgggagtt ccggtgtatt gctacgagat gtgcttctga
    25981 ggttagagct cgcgagctca gcgcgccggg tcagccgtga tccgcgttgg tgaggcacgg
    26041 cttgcaccga tgcgcaggca atcccaaccc ttcgattggg attcttgact gcgctcggtg
    26101 caagctgcgc cgcccgatca agacaaaatc ccagcggcgg acgccgcgag ccggattcat
    26161 tccgagcctg gactcagtgc caagcgcgcc cgtatcgagt attcaatcgc tggcagttgc
    26221 agtcttcctg ccgactattc tctcccatac ggcctgtcgt agtgcgtctg ccgctgctgc
    26281 gcgccaaggc gcgctggcga gagccgacgc tgccgcgcga ggtccaaaac gagagacttc
    26341 gtaatcgacc gcctccaccg cggcctcgag tgtggagaag cgctctttca ctcggccatc
    26401 caggctcacc tcccagcgtt ttccgcgtgc ggctcgcgtc aacacggtaa cctcgcatac
    26461 aacgccggga cgacgctcga aatgcagggc aacgccatgg cgacccagtg agaaccaggc
    26521 gttggccgag atggcacgca cctcatcgtg cgtctccaaa gtgagaggat tgaagctcgc
    26581 cgcgcgctgc tggatttccg gcaaggtgat gctcccggcc tcgagctcga agccgggatg
    26641 gtccaccaga atctgttgcc acacgcgggc cgcacgctcc gcgtcatctc cgcgcaaatc
    26701 gagctcacgg ggtgcgccga acaaggacgg tagactgcag aggctgagcc gtgaagcgtc
    26761 cacgaagtcc aagatgaggc agtctcgttt atcgttatga aggcgtgtgc cgcgtcccac
    26821 gcactgggca tacagccctt ctgagcgcgt aggccgagcc atcgcgatgc aggacacgcc
    26881 cggatcatcg aaaccctcgg tgagcaccgc gacgttgcac atcacttgta gcttgcccgc
    26941 cttgaattcc gatagcgccc ggcctcgttg ctcacccggc atatccccgt gcacgattcc
    27001 ggtggcgact ccgagcgcgt tcagcgcgta cgcgagattg cgtgcgtgcc ggaccgtaac
    27061 ggcaaacacg attgtgcgtc gatcccgtgc cagctcctga atcgaacgcg ctacgagcgc
    27121 gtttcgctcc tcgatgtcga ccgcctcctc cagctcgtcc tcgcggaagt cgagcccggt
    27181 cgcggagagc cgagtcagat cggccgcggt cgagatgcga aatccgcgca gtggcgcgag
    27241 atacccgtcc tggatcagct ccggcaagct gcgcgaatac acgatctgtt cgaaaatctc
    27301 gtcgagcccg cgaccatccc cccgcgctgt agttgcggtg aagcccaaca atgtaccggg
    27361 gaacttcgcg tcgaacgctc cgagttgtcg aagtacgcgc aggttgtcct ctgcggccgc
    27421 gtgatggcat tcatcgtaga tgatgaggcc caactgacgc tgccgcatca cttccgccaa
    27481 gcgggcttca tggagggagc ggatcgagca aatcaacacc ttggcatcgc gtgccccgcg
    27541 ctcactgcca cgctcgatag ctaccaggtg ctgtcccccg agttcgtgct ggagcttctc
    27601 gctcgcctga tagagtagct cctctcggtg cgcgagcacc aacacctgac gttgcgccaa
    27661 ctgggctagg cgcgagaaga ttacggtctt gcccgcaccg gtgggtagac agaccaatag
    27721 acgccgtacc ccgcggcgac gcgcagcaag caccgcgtcg atagcctgtt gttggtacgg
    27781 ccggagactc agcacgaaga acctactcag atgcgctcta gcacgaagaa cccttacccg
    27841 atgaacttac gcgcaacgcg ggtcacaaag ccggcggccc cgcatcgaat tgtggcaagt
    27901 catcggcgat ggtctcccaa ccagcttttg agcccacgtg gatgtgacga tcggggcgta
    27961 cgcctgggtc ctcgtcgaac gaacccatgg ggatgatacc aatcccgcgg ccctcgtcga
    28021 agcggggcaa cgtagaaccg caacggctac agaaccaatg ggcgaaatac ttcgcctcgg
    28081 gtaaccgata cttggtgagc aattcctcac cctcgaggta tcgaaagcca tcgagcggaa
    28141 ccaccaaatt caccgcgtga cccgtgccac gcgtcttgcg gcagcggctg caatggcatt
    28201 gcgtgacgcc tcgcggagcg gccgtcatct cgaatacgat agcgccgcag aggcaacttc
    28261 cctttacgct cgtcatagcc gacactctac gcgatccgct ccgagtcggg ataggggttg
    28321 gttacgctgg gttggtactc gaatgcagag ctggtcacct cgaatgaggc gtctcccatg
    28381 gccagtgatg caatctcatc gcacggcgga gccaccggcg cttcccctgc ctcgcccgat
    28441 gggccagccc cgcccgatac gccagcccct gcccgaggat ctggaaacct gaacgtcatc
    28501 gttcgtgaag tcgcggcccc agatatcgaa tctgctcttt tacagctttg gagccgtaca
    28561 ttcgttggac ctggacttgc gtgattgccc ccggccacgc cagcagcact ggacgtcatc
    28621 cgcctgtgcc cgcggtttcg tgagccaacg aaacgccgtg ccatgcccag tggggctggc
    28681 gggtcactcg cgagagcgtt gggcgcgcca cttgcagctc tggctcggcg aggattcatg
    28741 tctcgcaccc caccgcccgt cccgctattg ctcgtgcttt tgtgcctctg ctcgaccact
    28801 gtgacatgcg ggcgtgaagc accgcctgcc aacaaagccg ccccggtgca gccagcgctg
    28861 ctgggtgagg aaagcctggc aacggtgaag cgcgaagacc tagacaccgg tcccctcatc
    28921 agcggttccc tcgaaccgga acgccgcgcc gaaatcgtgg ctgaagcgag cggctccgtg
    28981 ctgaagctgc aagccgaact cggggatacg gttaaacgcg gccaggtgct ggcacgcatc
    29041 gaaacaactg gcatcgtcga cgcctatcgc tccgccaaag tcgcagaatc atcggcccaa
    29101 caagcgctcg acctcgccca acgcgagttc gaacggtctc gcagcctcgt cgaaagcggg
    29161 gccctgccaa cagcagactt ggaccgagcg cgcaacgcgg tggaggccgc gcaggcccag
    29221 ctcgccgata cgcaggctcg gaccgcgtcc acccaacggc aggtcgcgag cgcagtagcc
    29281 acgtccccgt tcgatggcgt ggtcagcgcg cagagcgttc acgagggcga cgtggtggcc
    29341 ccggggacac gcctcctcac cgttatcgat ccctctagcg tgcgtctcga agcccaagtc
    29401 gcctcttccg cgctacctgc gttgagcctc ggtgccagag tcagcttccg agttcggggt
    29461 tatcccaatc aaactttcac tggcctgatc cagcgcatcg cgcccgccgc tgacccggtc
    29521 acgcgccaaa tcacgttgtt ggtatcgatt cccaacccgc agagccagct cctttccggg
    29581 ctcttcgcgg aagggcgcgt cgcaagcgag caccgctccg cgctcgtcat ccccacggcc
    29641 gcggtcgaac gcgccgatca gggggcgagc gtcatagctc tacgacaagg caaggctcag
    29701 cgcgtagaga tcgagcttgg gctcgaggac gaagcgcgtg agatcgtcga ggtcaaatca
    29761 ggcctgaacg aaggagaaag ggtcgtgacc ggcgcggcgc gcaagctcac accgggcacc
    29821 cccgttcgcg aactcccgcg cccaaccacc cgcgccggca ctcactcgga aagcgagctc
    29881 gcgcagtcac aaggggcgga gtgagcatgt ggatatcgga tttctccatt cgcaaccgag
    29941 tggtcacgag cgtggttatg atcgcgctct tggttt
//
LOCUS       DASTKU010000574         8241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1897978, whole genome shotgun sequence.
ACCESSION   DASTKU010000574 DASTKU010000000
VERSION     DASTKU010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897978"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1781)
                     /locus_tag="VFQ61_19965"
     CDS             complement(<1..1781)
                     /locus_tag="VFQ61_19965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956787.1"
                     /translation="MFVWSRRSAQTVLVGAVWAVLLPVLPGACSLPDYEFSTSTERQS
                     CDRNAQCEPLSATKLCDTKAGKCVECLPSAASSGCGPGQYCDSNRCSVGCSSNDECPT
                     GLECDVESHQCRGCEDGATDACAVGTSCKDGRCVPSCENAEDCPAGFSCCNGACANPA
                     SDPSHCGRCGNVCGVAEDCINGACGTSGLCRSGRGDCDGRADNGCETVLNDPVANCGD
                     CNVTCAPPHATAECSTGTCRVASCDPGYADCDPQILGCETPLGTTENCAECGDSCKPT
                     NAESNACAEIEGGWTCRPRCIESFDDCDGKPSNGCETDLRSPEHCGACNHRCSLKNTS
                     TYTCEDGTCLVASCSPGFDNCDDNSSNGCELPVRDDPMNCGKCKRQCSNVNGTGSCVD
                     GQCQIACKADFGDCNEDNADGCEQDLRSSTRYCGDCKNTCTEPFCVNGVCKDHLDIVV
                     MNSTSHDIRASNGSSQVTLAVTHQLQSAPSPRRLILVGVATMGNSAPAPSAVLYQNMP
                     MEPLQTIQSNNQVWTGIYGLIESKLAPGAYQVSVQVNAAFAVGANVVELNNVEPSGLN
                     TQIGGSKHGNCTNLFDDIEVATGGSFV"
     gene            2378..5884
                     /locus_tag="VFQ61_19970"
     CDS             2378..5884
                     /locus_tag="VFQ61_19970"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9956788.1"
                     /translation="MTSDVDDTDALATARQSLHGSEERFRLLVEQVTEYAIFLLDPQG
                     RVSSWNLGAARIKGYDSSEIIGRHFSKFYRPEDRWKCDLELQIAERAGRVEDEGYRVR
                     KDGSLFWANVVITCLRDLNGGIVGFAKVTRDMTERRKAAESLRQSEERLRLIVESVKD
                     YAIIMLDDLGHVSTWSPGAEGIYGYQAQEIMGQHVSRFLPDRAATPGQAGEEAGGEAI
                     ELQRARRDGRFEDEGWRVRKDGSRFWANLVVTALRDTKSSVQGFALITRDLTERHRAE
                     EARLRLGQAARERINILSELAVALAGALSVEEVSRIVAEKGTTLAQADTCTVYLLNER
                     TGDLELFAERGCNPAVLPYIQRITPDSGQPTYAIGAGQAGALWVETAEQYAALIPSLA
                     NLKTDGPRAQAFWCVPLVAERRTLGMLGVGFHRPREFSDEERDFVATFARHCAQSIAR
                     ARRLEAERAAAALADRLRASLATTLRSIGDALIATDQNGAITMMNGVAEALTDWTEAE
                     ARGHPLPDVFHVINEHTRQLVANPVERVLETGGVVGLANHTVLISRAGREIPIEDSGA
                     PIRNDRGAIEGVVLVFRDVSERKHEESRQAFLADATSVLAGSLDYEQTVAQVAQLAVP
                     RLADWCAVDLIVEGERAPRRLAVAHVDPAKVHLAKELNAKYPPNPDAPAGVPNVLRTG
                     RSELYPEIPDELLVARCVNEDHLHIARELGLRSALMVPLMAQGRVLGAMSFIYSTSGR
                     TYTEEDLRFAEELARRCANAIENARAYRAEQKARRGADIANRAKDEFLAIVSHELRTP
                     LNAIMGWAKMLTTREFDERRGRSALETIERNAIAMAQLIEDLLDMSRVISGKLRIEVQ
                     QVEVARVVDAAIESIRPAAFAKGIELVTSLDRNVPAIMGDPTRLQQIVWNLLSNAVKF
                     SPKGGRVQVLLRESGTALEVSVTDDGKGIAPEFLPHVFDAFRQEDASPSRSRGGLGLG
                     LAITRQLVELHGGQIIAHSEGEGRGATFSVFLPYSVANPVVERAERATREFLESPTFP
                     RPEHIRGLRVLAVDDDEDARHLVATILEECGCSVTMADSVEDGMRKFQAEVPDILLSD
                     VAMPGEDGYELIRRVRALPHHQGGDVPAAALTAYARAEDRRSLLNAGYSIHLPKPIEP
                     AELVAVVATLSRFLPRAHKPES"
     gene            complement(6002..7663)
                     /locus_tag="VFQ61_19975"
     CDS             complement(6002..7663)
                     /locus_tag="VFQ61_19975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956789.1"
                     /translation="MSLVSANSKWISQRFVAPRWLPLIAACGEALVRHAVPIIYVAWF
                     ITTAAAQLWAWGVDPVLFPSPDEAVNRLAAQLVSKQGNPFITLPFRDAEDLVHMRLWI
                     SVGNRALPVYPPVTYYFYGLLLRLGEVGLWLIASLPAAGIGAFAAGVARLMSPKRRWL
                     GLLAPALAFPATYWIVRPWMNISPMLVAACWALFFWANWREKAKDGWLYACFAAVAFA
                     AAVRPDYAPYLLTASVLFSLAAAPAAWRRIFAAGFAAGAFALGLNLVLNKLTTGNAFR
                     VAYQLVSARETGQVSRSGPLHLFVELLFPVGFPPWEVVWQLIRKYWIQLLPLPALLLA
                     QLAVVPLLFKQARLQMALYAAAVCVVVCVMFARMNPEAFGSDDAVGRLSHTVPRYWTL
                     VYLLAALPPLLLLGRWQWRSRLGAAAIGFGAAAACVLAVLGGLEIYSRQQTSLVYLER
                     MRHDRARWLQNAGRHIPKGALVYTGTSDKLIWSRWSVGMIDDPVSTASSMRRAVDAGL
                     PVYIAEPQYPRAKWRRALERALREQELSVETEDRRSHVYRVDNSE"
     gene            complement(8038..>8241)
                     /locus_tag="VFQ61_19980"
     CDS             complement(8038..>8241)
                     /locus_tag="VFQ61_19980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956790.1"
                     /translation="GHVLHLDHRTLAEITLDDASYERSIARVDSAFAAFESLLDTMYT
                     SALERSPARPLRGDWAQGNSLAP"
BASE COUNT         1556 a         2596 c         2625 g         1464 t
ORIGIN      
        1 tatacgaatg agccgcccgt cgcgacttcg atgtcgtcga acaaattggt gcagttgcca
       61 tgtttgctgc cgccaatctg ggtgttcagc ccgctgggct ctacgttgtt cagctccacg
      121 acgttggcgc ctaccgcgaa cgcggcattg acttgcacgg aaacctggta cgcgcctggt
      181 gcgagcttcg actcgattaa tccgtaaatt cccgtccaaa cttggttgtt ggactggatc
      241 gtttgcagcg gctccatggg catattttga tacagcaccg cgctcggcgc gggcgcgctg
      301 ttgcccatgg tggccacccc caccaagatc aatctgcggg gcgatggggc gctctgcagt
      361 tggtgcgtca ccgcgagggt gacttgggat gagccgttcg atgcgcgaat gtcgtggctc
      421 gtgctgttca tcaccacgat gtccaggtgg tccttgcaga caccattcac gcagaacggt
      481 tcggtgcagg tgttcttgca gtcgccgcag tatcgcgtgc tgctccgcaa atcctgctcg
      541 cagccgtcgg cgttgtcttc gttgcagtca ccgaaatcgg ctttgcacgc gatctggcat
      601 tgaccatcga cgcagctgcc ggtgccattg acattgctgc actgccgctt gcatttcccg
      661 cagttcatcg ggtcgtcgcg cacgggcagc tcgcagccgt tcgaggagtt gtcgtcgcag
      721 ttgtcgaagc cagggctgca gctcgcgacc aagcacgtgc catcctcgca agtatacgtg
      781 ctggtgtttt tcagggagca acggtgattg caggcgccgc agtgctccgg ggatcgtaag
      841 tcagtttcgc agccgttgct cggcttgccg tcacaatcgt cgaaggactc gatgcagcgc
      901 ggtcggcacg tccagccgcc ttcgatttcg gcacaggcgt tcgattccgc attagtaggc
      961 ttgcagctat cgccgcactc agcgcagttc tccgtggtgc cgagcggcgt ctcgcagccc
     1021 aatatttggg gatcacaatc ggcgtacccg ggatcgcaac tcgcgacccg gcaagtgccc
     1081 gtggagcact ccgctgtggc gtggggaggt gcacaagtga cgttgcagtc accgcagttc
     1141 gcgaccggat cgttgagcac cgtctcgcag ccgttatcgg cgcgcccgtc gcagtcgccg
     1201 cgcccagagc ggcagagccc actcgtcccg caggcgccgt tgatgcaatc ctcggccacc
     1261 ccgcatacgt tgccgcagcg cccgcaatgg gacggatcgc tggcgggatt ggcgcatgcg
     1321 ccgttgcagc agctgaagcc ggctgggcag tcctcggcgt tctcacagct cggcacgcaa
     1381 cgcccgtctt tgcagctcgt gccgaccgcg caggcatcgg tcgctccgtc ctcgcagcct
     1441 cgacattgat ggctctcgac gtcgcactcc agcccggtag ggcattcgtc gttcgagctg
     1501 cagccgacgc tgcaacgatt gctatcgcaa tactgcccgg gaccacaacc gctgctcgcg
     1561 gcgctgggca ggcactccac gcatttgccc gccttcgtgt cacacagctt ggtcgcggag
     1621 aggggctcac attgcgcatt tcgatcgcag ctttgccgct ccgtgctggt ggagaactcg
     1681 tagtcgggaa ggctacaagc gccgggcagc accggaagca acacggccca gacggccccg
     1741 acgagaaccg tttgcgcaga tcggcgcgac cagacgaaca tctttccgag cgtacaacat
     1801 cgtcccaaat cgcaccaaca gctggcaagc aagcgccatc gagcgaacac gccaggcagc
     1861 cggacatccg gagctcacca gctttcagca ttgcctgggt tgaccttgcc tgggctgact
     1921 ctgcctggct tgaccctgcc ttgggctgac acgggcttgc gctggcatgc aggacatggt
     1981 ctcaggccca aagcggtgtc cgaaaccggg cgccgaggtg cgctacgagg tgcgctacga
     2041 ggtgcgctaa gctcggcgga aagatcctgc cagcccggtc tttcatcggg ctgaggcctt
     2101 cgcgctcacg ccacgtctca ttcggagtct cctccggccg cgaaccggct tgagtcaccg
     2161 catgactcgc gagggtgaaa aggtcgcacg agtggagtga gattggggcc tgccgagcgg
     2221 tgatgctccc gtcgggcgca atcggcgggg taaagcgcgc gcgttctggc tccaaatccc
     2281 cagcgcaact gaccgggatt gttctcgcct ttgtgacatt ttgcaccttc ggccgtccat
     2341 gcctttcgag taggttctga ctataagagt caggctgatg acaagcgacg tggacgacac
     2401 tgatgcgttg gcgacggctc ggcagtccct gcatggtagc gaagagcgct tccggctcct
     2461 ggtcgagcag gtcaccgaat acgcgatctt cttactcgat ccacaaggcc gggtgagctc
     2521 ctggaacctg ggtgcagcac gcatcaaggg ctacgactcg agtgagatta tcggccggca
     2581 tttttcgaag ttctatcgcc cggaggaccg atggaagtgc gacctggagc tccaaattgc
     2641 ggagcgcgca ggtcgcgtcg aagacgaggg atatcgtgtt cggaaggatg gttcgctgtt
     2701 ctgggcgaac gtcgtgatca cgtgtttgcg cgacctgaac gggggaatcg tcggcttcgc
     2761 caaggtgacg cgggacatga cggagcggcg caaggccgct gagtccttac gccaaagcga
     2821 ggagcggctg cgactgatcg tcgagagcgt caaagattac gccatcatca tgctcgacga
     2881 tctgggacac gtgtcgactt ggagtcctgg cgcggaaggc atctacggct accaggccca
     2941 ggagatcatg ggtcaacacg tctcgcgctt tttaccggac cgagccgcga ctccaggcca
     3001 ggccggtgag gaagctggcg gcgaggcgat cgaactccag cgtgcgcggc gcgacggtcg
     3061 cttcgaagac gagggttggc gcgtccggaa agacggcagc cgtttctggg caaacctcgt
     3121 tgttacggcg ctgcgcgaca ccaagtcttc ggtgcaaggc tttgccctga tcacgcgcga
     3181 tttgacggag cgccaccgcg ccgaggaggc ccgcttgcgg ctcgggcaag cggcgcgaga
     3241 gcgcatcaac atactgtccg aactcgcggt ggcgctggcc ggggcgctct ccgtggagga
     3301 ggtcagccgg atcgtggccg agaagggcac caccttggcc caggccgata cgtgcacggt
     3361 gtacttgctc aatgagcgca ctggagatct cgagttgttc gccgagcgcg gttgtaaccc
     3421 ggcggtgctt ccgtacatcc agcgcatcac ccccgactcg ggccagccca cgtatgcgat
     3481 cggcgcggga caggccggcg ccctgtgggt ggagacagcg gaacagtatg cggcgttgat
     3541 tccctcgctc gccaatttga agacagatgg gccgagagcg caggctttct ggtgcgtgcc
     3601 tttggttgcc gagcgtcgca ccctgggcat gctgggagtc ggtttccatc gcccacgcga
     3661 gttctcggac gaggagcgcg atttcgttgc cacgtttgcg cgacactgcg cccaatcgat
     3721 cgcccgcgct cgacgcctgg aagcggagcg agccgcggcg gcgctcgcag atcgactgcg
     3781 cgcctcgctc gcgactaccc tgcgcagcat cggcgatgcc ctgatcgcta ctgaccagaa
     3841 cggagccatc acgatgatga acggggtggc ggaggcgctg acggattgga cggaagcgga
     3901 ggcgcgaggt caccctctgc ccgacgtgtt ccacgtcatc aacgagcata ctcggcagct
     3961 cgtcgcgaat ccggtggagc gagtactcga gaccggcggc gtggttggcc tcgccaatca
     4021 cacggtcttg atatcgcgcg cgggccgcga gatcccaatc gaggatagcg gggctcccat
     4081 ccgcaacgac cgtggcgcga tcgagggcgt agtgctggtg ttccgcgatg tcagcgagcg
     4141 caagcacgag gagtcgcgac aggccttcct ggcggacgca acgagcgtgt tggcgggctc
     4201 cctcgactac gagcagacgg tggctcaagt ggcccagttg gcggtcccac gcttggcgga
     4261 ctggtgcgca gtggatctca tcgttgaggg ggagcgcgcg ccgaggcgtt tggccgtcgc
     4321 tcacgtcgac cctgcgaaag tgcacctggc caaggagctc aacgccaagt acccgccgaa
     4381 cccggacgcc ccggccggag ttccgaacgt gcttcgcacg ggacgctccg agctctatcc
     4441 agaaatcccc gatgagctcc tggttgctcg ctgcgtgaat gaggaccacc tgcacatcgc
     4501 ccgcgagttg gggctgcgtt ccgccttgat ggtgccgctc atggctcagg gccgagttct
     4561 cggtgcgatg tcattcatct actcgacttc gggccgaacc tatacggagg aggatctccg
     4621 cttcgcagag gaactcgcgc gccgctgcgc caacgccatc gaaaacgcgc gagcttaccg
     4681 ggccgagcaa aaggcgaggc gcggcgccga catcgcgaac cgagccaagg acgagtttct
     4741 ggcgatcgtc agccacgaac tgcgcacgcc actgaacgcc atcatgggct gggccaagat
     4801 gctgacgacg cgggagttcg acgagcggcg ggggcgcagt gctctcgaga ccatcgagcg
     4861 caacgccatt gcgatggcgc agttgatcga ggacctgctg gacatgtccc gggtgatcag
     4921 cgggaagttg cggatcgagg tgcagcaggt ggaagtcgcg cgcgtggtcg atgcagccat
     4981 cgaatcgatt cgccccgctg ccttcgccaa gggcatcgag ctcgtaacgt ccctcgatcg
     5041 caacgtgcct gcgatcatgg gtgacccgac gcgtctgcag cagattgtct ggaacttgct
     5101 cagtaacgcg gtgaagttca gccccaaggg cggccgggtg caggtccttc tgcgggaatc
     5161 ggggacggcc ctcgaggtct ccgtcaccga cgacggcaag ggcatcgcac cggagttcct
     5221 gccgcacgtg ttcgatgctt tcaggcagga ggacgcaagc ccatctcgct ctcgcggcgg
     5281 tctgggcttg gggctcgcga ttacgcgtca gctcgtcgaa ctccacggcg gacagatcat
     5341 cgctcacagc gaaggggagg ggcgcggagc gactttctca gtgtttctgc cgtactcggt
     5401 ggctaatccc gtggtcgaac gcgcggagcg tgcaacccgt gagtttctag aaagccctac
     5461 atttccgcga cccgaacaca ttcggggtct acgggttctc gccgtcgatg acgacgagga
     5521 cgctcgccat ctggtcgcga ccatcctcga agagtgcggc tgcagcgtga cgatggccga
     5581 cagcgtcgag gatggaatgc gcaagttcca ggcggaagtg ccggacatat tgctgtctga
     5641 cgtcgcgatg cccggcgaag acggttacga gctcatccgc cgggtgcgcg cgttacctca
     5701 tcatcaaggg ggtgacgtac cggccgcggc cctaaccgct tacgcacgcg cggaagatcg
     5761 tcgaagcctg ctcaacgcgg gctactcgat tcacctaccg aagcccatcg agcccgcaga
     5821 gctggtcgcg gtggtcgcca cgctgagccg cttcttgccg cgcgcacaca agccggagag
     5881 ttagtcccgc taccagcacc tttgctgcgg accacgctcg cagatccggt tttgacctcc
     5941 attggggtgc cggttcggtg gtccccaaca cgtcgtatgc cccgctccga tgctcgccca
     6001 atcactcgct gttatctacg cggtacacgt gcgagcgccg atcctcggtc tccaccgaga
     6061 gctcctgctc acgaagcgcg cgttctaggg cacgccgcca cttcgcccgc gggtattggg
     6121 gttcggcgat gtacaccggc aatcccgcat cgaccgcccg gcgcatggaa gacgccgtgc
     6181 tgacggggtc gtcgatcatc ccgacggacc aacgtgacca aatcagcttg tcgctcgtcc
     6241 ccgtgtagac gagcgcaccc ttcggaatgt gccggcctgc attctgcaac caacgagctc
     6301 ggtcgtgacg cattcgctcg agatagacga gtgacgtttg ctggcgcgaa tagatctcca
     6361 ggccgccgag caccgcaaga acgcaagcgg ccgctgctcc gaacccaatt gcggcggctc
     6421 ccagcctgga gcgccactgc caacgcccga gcaacaacag gggcggcagt gccgctaaca
     6481 gatagaccag ggtccaatat cgcggcacgg tatgtgaaag cctgccgacc gcgtcatcgc
     6541 tcccaaaagc ctcggggttc atgcgggcaa acatcacgca gaccacgacg cacacggccg
     6601 ccgcatacag cgccatctgc agccgagctt gcttgaacag cagtggcacg accgcgagct
     6661 gagcgagcaa gagagccggc aaaggcaaga gctgaatcca atacttgcgg atcaactgcc
     6721 acactacttc ccacggcgga aagccgaccg ggaagagcag ctctacgaac aggtgtagcg
     6781 gccccgagcg cgatacctgc cctgtctccc gtgcactcac cagctggtac gccactcgaa
     6841 aagcgttgcc ggtggtgagc ttgttcaaga cgaggtttag ccctagagca aacgcaccgg
     6901 ctgcgaaccc ggcggcgaag atcctgcgcc aagctgccgg agcggccgcc agcgaaaaca
     6961 ggacgctcgc ggtgagcaag tagggcgcgt agtctggacg aacggcggcg gcaaaggcga
     7021 ccgcggcaaa gcaggcatac agccagccat cttttgcctt ctcccgccag ttcgcccaga
     7081 agaataacgc ccagcaggcg gccaccagca tgggcgagat gttcatccag ggacgaacaa
     7141 tccagtacgt ggccggaaag gcgagcgccg gtgcaagtag gccgagccag cgccgcttag
     7201 ggctcatcag ccgagccact ccggcggcga aagcgccgat ccccgcagcg ggcaagctcg
     7261 caatcagcca gagcccgacc tcgccgagac gcaagagcag gccgtaaaaa tagtaagtga
     7321 caggcggata aactggcaac gcgcggtttc ccacgctgat ccagagccgc atgtggacca
     7381 aatcttccgc atcgcggaac ggcagggtga tgaacggatt gccctgctta ctcaccagct
     7441 gagcggcgag acgattgact gcctcgtccg gagacgggaa cagtacggga tcgacgcccc
     7501 aagcccacag ctgtgccgcg gcggtggtga taaaccaagc aacataaatg attggcacgg
     7561 cgtgccgaac cagggcctcc ccgcaagccg cgatcagtgg cagccagcgc ggcgcgacga
     7621 atcgctgcga tatccacttg gagtttgccg aaaccaggct catacagcgc cctcgtccaa
     7681 acgcgaccaa cggcgctttt gcacgttcgg gagccttcgt caacgctcga ttcccaatcg
     7741 gaagtgaacc caaccgcgtc caggcatcca gctgccgcag tggcaaggca actgaaagtc
     7801 cgcctagaca cccgccgggc ctttcgaaac cttgcttcaa gtccgagata aacaggcttg
     7861 gacgacccaa gtttttcggg gttccccttc aaatatacgt tgcgcctcga atgcttagac
     7921 ccccccccgt gccctagcga aacgggacgg gctagaccca agaatccacg gctccgaagc
     7981 ccagcgagtg ggcccagcca agttggaccg aggtcgagcc ggccgattga gctcagtcta
     8041 cggggcgagt gagttacctt gggcccagtc accgcgaagg ggtcgagcgg gtgaccgttc
     8101 gagtgcgctc gtgtacatgg tatcgagtag agattcgaat gctgcaaatg cgctgtctac
     8161 tctcgcgatg ctgcgttcat agctggcatc gtcgagcgtg atttccgcca acgtgcggtg
     8221 gtcgaggtgc agcacgtgac c
//
LOCUS       DASTKU010000575         7506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_334767, whole genome shotgun sequence.
ACCESSION   DASTKU010000575 DASTKU010000000
VERSION     DASTKU010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334767"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2521
                     /locus_tag="VFQ61_19985"
     CDS             <1..2521
                     /locus_tag="VFQ61_19985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956791.1"
                     /translation="LDEAEFTEVTDSPPAGLNMSRAPLEHEVVTGEFEEFEEFEEFED
                     DEEVPTRVHQAAPREWIQAPGQSAGAPQAAAPAKQPISSPRAAVVPAVNRGASSVRAD
                     AAVPSATPSVTASPARLPSPPTALGNVAPRSWEPQAPSSIPSRAGAAASPSAQPARSP
                     LPSIRTGAPVGSSAAAFAPTAGFAVPEAMSDAGRNLPTDLGRNQLTRSDDPRYPAPSI
                     LLQHLARVGVFEPSGGALPVWEAAPQHKSRGVVPLVTAILLVLAAGGGAYHYSKLRKV
                     ERLAQASTLNREVSQLLHSARVSDLRATDDKLSRVFDLDSRSREAARLWLENRVLAAL
                     LTGEEPQGIDSAVHRGREVGLTEPELAVGRMASFFVEGDLAGAAALLPKWDAKAATDA
                     LYQVMAGAVLERAGDARSLERYAAARALDPKLVLADLLYVRAALLEGESEPALKALAE
                     LQGKVPEGEASARVLRALAFAVGASSEGAAREALPPAEVERLPAALRALPHYVEAVAA
                     VERADAAGARAAIEASLVQVHEPALAVRLGQLGVELGDELLARKAALKALSFSGTHAG
                     ARALAARVALLGGRLDEGQKAVEELDPASKDVAIVHAAVAYESSDAPNVASTLAAFGD
                     VAKTTELSALSQGADLILGVRYPAANSLEALASPKVAWGELIALDAALDTGNLEFADR
                     VVSRRDPASLGPVHLLRVARLRRYQKRTDEALAASESALSAKPAAALVAERILSLVDK
                     QQVQPARELLLKHNAVLGARAGWLGVLVDLAAKQPVQAAARLAQLEPPPEDAPIQLRV
                     LAGRALAEATDRRARPFVGILFKRAAKHPDAMIAALALRGH"
     gene            complement(2598..4337)
                     /locus_tag="VFQ61_19990"
     CDS             complement(2598..4337)
                     /locus_tag="VFQ61_19990"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET9956792.1"
                     /translation="MSRAIWLGLSIFASAPLFGCGGDSATPPRGAAGNTSAGGNAGSG
                     NGGNAPTAGTSPNGGNGTGGASGGTAPIGDPTKECAAPDKGAPLLRRLASRELSRALE
                     DIFPEAKGKWSTSLTTDLISHRGFDNDADVLVVGKQTADQITDTAKAVADAITGDALN
                     QVLPCAASSPGASCAGEFLSKYGKRLFRRALTPAETTSYTEFFTLAASKTDFKQGLGW
                     LIRGLIESPNFLYRRELGAVAGGVRQLTQHEIATELAFTFGGTTPSDALLAQADRGEL
                     ATPEQRTQVAKTLLSSAGGREVIQTLFNSIFGYSRVTSVAKTNVSEFAAQRDEMVQET
                     RRFIEEVVVNKAGGPKELLTAAYSTPSSKLASFYGWPAPSSDYAVTQRPSGRGVGILA
                     QGSVLASRALPNGSSPTKRGLLVFEKLLCREPDPVPPSIPMLPEPKAGVTTRERYETQ
                     HAAGGCQVCHSKFDPIGFGFEHFNEAGQFREKELVPGSNQALDINATGTVKDDAGTAF
                     TFDGLEQLAMQLAQMPEVYSCMSGDVSAFAFGTTESCLAQTRRAEFVSGKLSFVDYLT
                     SLAAEPHFTQRSP"
     gene            complement(4334..5785)
                     /locus_tag="VFQ61_19995"
     CDS             complement(4334..5785)
                     /locus_tag="VFQ61_19995"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9956793.1"
                     /translation="MKSNFWIDRAARRLQRRFFLKAVGLGLAAPLAMKLSQQAFAQSA
                     GRPKRFMLFYHPHGVPPEHFNPKVGSSPTDFSLTESGVSILGPLSEIKTQVNVLQGFK
                     YPGAMTHEGILTFLSGFGNGMGVDEQSPRTTVEHVIGNALGIKPLLLGAIPHRQWGID
                     KDGKLFWDKQPVVAEKNPLKAYDSVFGKLGGTSSTGPDPSVELNEALRQLTMKELTTL
                     QGEVAQITREKNKLQTHLDALASRASMGGGGGMGGGTMACSSKPTIAAVDALRTKAQG
                     QGEDFFVKEDNFPDIYAAQLGIAAHALLCNAAPIVGVQAMYTNAELDFKFMGSSGAHH
                     TTLSHTGPGFSNNGLSLNLETRTPFAKAQRWFYEQLAKHVLTVLKQPDPLDPTHTVLD
                     NTLIYCSSEIGEGAWHPSATVELKQAATAAALSYLPLVTIGGGGGAIKTGNVMKFSPD
                     RPASDIYLALARAMGVANATFPNSTGALTEILT"
     gene            complement(6311..7261)
                     /locus_tag="VFQ61_20000"
     CDS             complement(6311..7261)
                     /locus_tag="VFQ61_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956794.1"
                     /translation="MRCLPHLGAAWRTEMAARRDTSEISMRVTLLGKSLSLGSLGLAL
                     LSGTAWAQDATAPAPAAPAPAAPASPAPDTAPMPAPGAAPAAATPPAAPEPTTAEPAV
                     PEAKPAAPEAPVEESYPPAWFRIDSDLGNLQFWAGATHPVAEGLGIASDLYLVGTLGE
                     FDIGPAITVGPFTATPMLGFQVDWAKRKAQALVPQFYFTGGPDPVYLELWVQNYQNQL
                     FDSTPNHDLYTRFFVDYKFSKYVGIGPQIEPYFALNKKAADDSERKSGIMSMVVGGNV
                     MLSNYGKGNVVFLFLGYETKKDARPDDAGLGGRFTWVRNF"
BASE COUNT         1261 a         2398 c         2484 g         1363 t
ORIGIN      
        1 tttggatgag gcggagttta cggaggtcac ggattcaccg ccggcgggtc tcaacatgtc
       61 gcgcgcgccg ctggagcacg aggtggtgac cggcgagttc gaagagttcg aagagttcga
      121 agaattcgaa gacgacgagg aggtcccgac gcgagtgcat caagctgcgc cgcgcgagtg
      181 gatccaagct cctggtcaaa gcgcaggcgc acctcaggcg gccgcgcccg cgaagcaacc
      241 catctcatcg ccgcgcgcgg cggtggttcc ggccgtgaac cgaggggctt cctcggtgcg
      301 cgccgatgcc gcggtcccga gtgctacgcc cagtgtgact gcttcgccgg cgcgtctacc
      361 gagtccgccc accgcgctcg gaaatgttgc cccccgaagc tgggaaccgc aagccccgag
      421 ctccattccc tcgcgagccg gcgcggcggc gtcgccgagc gcgcaaccgg cgcgcagccc
      481 tctgcccagt attcgaactg gcgcgcccgt cggctcgtcc gcggcggcgt ttgcaccgac
      541 ggctggcttc gccgtgcccg aggcaatgtc ggatgcgggt cgcaatctgc cgactgatct
      601 cgggcgcaat cagctcacgc gcagtgacga tccacgctac cccgcaccgt ccatcttgtt
      661 gcagcatctg gcgcgcgtgg gcgtgttcga accctcgggc ggagcgttgc cggtatggga
      721 ggcggcgcct cagcacaaga gccgcggcgt ggtgccgctg gttacggcaa ttttgctggt
      781 gctcgcagcg gggggcggcg cgtatcacta ctcgaagctc cgcaaagtgg agcgcctggc
      841 gcaggccagc accttgaatc gtgaagtgtc tcagttgctt cactccgcgc gcgtcagcga
      901 tttgcgcgcg accgacgaca aactgagccg cgtcttcgat ttggattcgc gtagccgcga
      961 ggcggcccgg ctttggttgg agaaccgagt gctcgccgcg ctgctcacgg gcgaggagcc
     1021 tcagggcatc gactcggccg tgcatcgggg ccgtgaagtg ggtctcaccg aacctgaact
     1081 cgccgtcggg cgtatggcgt ctttcttcgt ggagggggat ctcgcggggg ccgcggcgct
     1141 gctgccgaag tgggacgcaa aggcggcgac cgatgccttg tatcaagtca tggccggcgc
     1201 cgtgctcgaa cgcgcgggag acgcgcgctc tctcgagcgt tacgcggccg cgcgggcact
     1261 ggatcccaag ttggtgctgg cggatctgct gtatgtccgg gccgcgctgc tggaaggcga
     1321 aagcgagccc gcgttgaagg cgctcgcgga gttgcagggc aaggtaccgg aaggtgaggc
     1381 tagtgcgcgc gtgcttcggg cgctggcttt tgccgtagga gcgagctcgg agggggcagc
     1441 gcgcgaagcg ttaccgcctg cagaggtcga acgtttgccg gctgcattgc gcgcgttgcc
     1501 tcattacgtc gaggccgtgg cggccgtgga gcgcgcagac gcggcgggcg cgcgggcggc
     1561 aatcgaggcg tctttggttc aagtgcacga gcccgccttg gcggtgcggc tggggcagct
     1621 cggggtcgaa ctcggcgatg agcttttggc ccgcaaagcc gcgctcaagg cgctctcttt
     1681 ttcgggcacc cacgcgggcg cccgtgcact ggccgcacgc gtggcgttgc tcggcgggcg
     1741 cttggacgag gggcagaaag ccgtcgagga gctcgacccc gcctcgaagg acgtcgcgat
     1801 cgtgcatgcg gccgtcgctt acgagtcatc cgatgctccc aacgtggcca gcacattggc
     1861 cgccttcggg gatgtggcca agaccacgga attgtccgcg ctctctcagg gagcggactt
     1921 gatcttgggc gtccgttatc cggctgccaa tagcttggag gcgctcgcgt ctcccaaggt
     1981 cgcttggggc gagctcatcg ccttggatgc ggccctagat acgggcaacc tagagtttgc
     2041 cgatcgtgtg gtgtcgcggc gcgacccagc ttcgctcggc ccggtccacc tgctgcgcgt
     2101 ggcgcggctg cggcgctacc agaagcgaac cgatgaggcg ctggccgcga gcgagagcgc
     2161 gctctctgca aaacctgcgg ccgcgctcgt agcagagcga atcttgtcgc tggtggacaa
     2221 acagcaggtt cagcccgcgc gcgagttgtt gctcaagcac aacgcggtgt tgggtgctcg
     2281 ggcgggctgg ctcggtgtgc tcgtagactt agccgcgaag cagccggtgc aagccgcggc
     2341 gcgtttggct cagctcgaac ccccgccgga ggacgctcca attcagctcc gtgtcctcgc
     2401 cgggcgtgcc ctcgctgaag ccaccgatcg ccgcgcgcgt cccttcgtgg gcattctatt
     2461 caagcgcgcc gccaagcatc cggacgccat gatcgcggcc ctggcactcc gaggtcactg
     2521 agctcagtgg cctcggagct ccgagcgccc tttcggattg gcgctcggca atcagcggct
     2581 cctggtgtga taactgctca gggtgagcgc tgcgtgaagt gcggctcggc ggcgagcgac
     2641 gtcaggtagt cgacgaagct cagcttgccg gatacgaact ccgcgcgtcg tgtctgcgcc
     2701 agacacgact ccgtcgttcc gaaggcgaac gcgctgacgt ctccgctcat gcacgagtac
     2761 acctcgggca tttgcgccag ctgcatcgcg agctgctcga ggccgtcgaa cgtaaacgcc
     2821 gtgcccgcgt cgtccttcac cgtgccggtg gcgttgatgt cgagcgcttg attcgagccg
     2881 ggcactagct ccttctctcg gaactgaccc gcctcgttga agtgctcgaa tccaaaaccg
     2941 atcggatcga acttcgagtg gcacacctgg cagccgcccg ccgcgtgttg cgtctcgtag
     3001 cgctcgcgcg tggtgacgcc cgctttgggc tccggcaaca tcgggatcga gggcggaacc
     3061 ggatccggct cccgacacaa caacttctcg aacaccagca ggccgcgctt ggtaggtgac
     3121 gagccgttcg gcagcgcgcg gctcgccagc accgagccct gcgccaggat gccaacgcct
     3181 cgacccgagg ggcgttgcgt gaccgcataa tcggagctcg gcgcgggcca gccgtagaac
     3241 gacgcgagct tggaagacgg ggtgctgtac gcagccgtga gtagctcctt cgggccgcct
     3301 gctttgttta cgaccacttc ctcgatgaag cgtcgcgtct cctgcaccat ctcgtcgcgc
     3361 tgagccgcga actcgctgac gttggtcttg gccaccgagg ttacccgcga atagccgaag
     3421 atcgagttga acagcgtttg gattacctca cgtccgcccg cggagctaag cagagtcttt
     3481 gcgacctgcg tacgttgttc aggcgtagcg agttctccgc gatcggcctg agccagcagt
     3541 gcatccgacg gcgtggtgcc gccgaacgtg aacgcgagct cggtcgcgat ctcgtgctgc
     3601 gtgagctgcc gcactccgcc ggcaactgcg ccgagctcgc gccgatagag gaaattcggc
     3661 gactcgatca ggccgcgaat caaccagccg agcccttgct tgaagtccgt cttcgaggcg
     3721 gccagcgtga agaactccgt gtaactcgtg gtctccgccg gcgtgagggc gcgccggaac
     3781 aagcgcttac cgtacttgct caagaactca cccgcgcaac tggcgccagg gctggaggcc
     3841 gcgcaaggca acacctgatt gagggcgtct cccgtaatcg catccgccac ggctttggcc
     3901 gtatcggtga tctgatcggc agtctgctta ccgaccacga gcacgtccgc gtcgttgtcg
     3961 aagccgcgat gggaaatcaa atcagtcgtc agcgaggtgg accacttccc cttggcctcc
     4021 gggaaaatgt cctccagagc gcgcgacaac tcacgggacg cgagccgtcg caggagcggc
     4081 gcccccttat ccggtgcggc gcactccttg gttgggtcgc cgatgggtgc agttcccccg
     4141 ctcgcgccgc cggtgccatt gccgccgttg ggcgaggtgc ccgcggtcgg agcattgccg
     4201 ccattgcccg agcccgcgtt gccgcccgcg ctggtgttgc cagcagcgcc gcgtggcggt
     4261 gttgctgaat cacccccgca gccgaacagc ggcgcggacg cgaagatcga gagccccagc
     4321 cagattgcgc ggctcatgtc aaaatctccg tcaacgcgcc ggtgctgttg gggaaggtgg
     4381 cgttcgccac gcccatcgcg cgggcgaggg ccagatagat gtccgacgcg gggcgatccg
     4441 gagagaactt catcacattg ccggtcttga tcgcgccgcc tccgccgccg atcgtcacga
     4501 gcggcaagta ggagagagcc gccgccgtgg cagcctgctt gagctccacc gtagcagaag
     4561 gatgccacgc gccttccccg atctcgctcg agcaatagat gagcgtgtta tccagcacgg
     4621 tgtgcgtcgg atcgaggggg tctggctgct ttagaaccgt cagcacgtgc ttggcgagct
     4681 gctcgtaaaa ccaacgctgc gcctttgcga acggcgtgcg ggtctcgaga ttcagactca
     4741 agccgttatt gctgaatccc ggccccgtgt gcgaaagcgt cgtgtggtgc gcgcccgacg
     4801 agcccatgaa cttgaagtcg agttcggcgt tcgtgtacat cgcttgaacg cccacgatcg
     4861 gcgcagcatt gcacagcagg gcgtgggccg cgatgccgag ctgcgccgcg taaatgtcag
     4921 gaaagttgtc ttccttgacg aagaaatcct cgccttgacc ctgagccttg gtgcgcagcg
     4981 cgtctaccgc ggcgatcgtc ggcttgctgc tgcacgccat cgtcccgccg cccattccgc
     5041 ccccgccgcc catactggcg cgggaagcca gcgcgtccaa atgggtctgg agcttgttct
     5101 tctcacgggt gatttgcgcc acttcaccct gcagtgtggt gagctccttc atggtgagct
     5161 gacggagcgc ctcgttcagc tcgacgctcg ggtcaggccc cgtgctgctc gtaccaccca
     5221 gcttcccgaa cactgaatca taggctttga gcgggttctt ttcggcaacg acgggctgct
     5281 tgtcccagaa caacttgccg tctttgtcga tgccccactg gcgatgcggg atcgcgccaa
     5341 gcaacaaggg tttgatcccg agagcattgc cgatgacgtg ctcgacggtg gtgcgtggtg
     5401 actgctcgtc gacgcccatc ccgtttccga acccggaaag gaacgtcagg atcccttcgt
     5461 gcgtcattgc gcccgggtac ttgaagccct gcagcacgtt gacctgggtc ttaatctcgg
     5521 agagtggccc caaaatgctg acgccgcttt cggtcagcga gaagtccgtg gggctcgatc
     5581 caaccttcgg gttgaagtgc tcggggggta ctccgtgtgg gtgatagaag agcatgaagc
     5641 gcttcggccg acccgcggac tgcgcgaaag cctgctgcga cagcttcatg gccaatggcg
     5701 cagcgagacc caaacccacc gcttttagga agaaccgccg ctgcagccga cgcgccgcgc
     5761 gatcgatcca aaagttcgac ttcataagcg actactccgc atccgcacac acaaacgcca
     5821 cgacgagagg gcgaaaaact ctcgacccgc gaaccgctcg cagaaaagtt gtatctgcgg
     5881 aagctcgaac cgcaaggggt tcttcgcgaa gcgcgtgcag atttgtggcc ccctcgagtc
     5941 gcccaaggcc gcaatgcccc actcaccgaa tgttgggccc tttcccaatg agtgatgggc
     6001 cctgccggaa gcggcccacc tagcgttcgt ctgcgtgctc ccgagctccg gtcaccagcg
     6061 attccctcgc aggtgaccac cccaccaccc tcggagcccg ccttccttct atatggattt
     6121 cggcaactaa gaaccgagcg tcttggctca gtcttgccga aatcgcgcaa gcccaatcct
     6181 tgcgctttca gtcttctgat tcgttgtcga ggcgccctgc cctgccaggg cacctcgatc
     6241 tcgaccactc acactcctag atagcaacct gccgtgatgg agcgaacgac tcagagttgt
     6301 ggaccacgac tcagaagttg cggacccagg tgaagcggcc gccgaggccc gcgtcgtcgg
     6361 ggcgtgcatc cttcttcgtc tcgtagccga ggaacaagaa cactacgttg cccttgccgt
     6421 agttcgatag catcacgttg ccacccacga ccatgctcat gatgccagac ttgcgttccg
     6481 aatcgtccgc cgccttcttg ttcagcgcga aatacggctc gatttgaggc ccgatcccga
     6541 cgtacttgct gaacttgtag tccacgaaga agcgggtgta gaggtcgtgg ttgggagtcg
     6601 agtcgaagag ctggttttgg tagttctgaa cccagagctc gaggtaaacc gggtcgggac
     6661 caccggtgaa atagaattga ggcaccagag cctgcgcctt gcgcttggcc caatcgactt
     6721 gaaagccgag catcggagtc gccgtgaacg gccccaccgt gatggccgga ccgatatcga
     6781 actcacctag cgtccctact aggtagagat cggaggcgat gcccaggccc tcggcgacag
     6841 ggtgagtggc acccgcccag aattggaggt tgccaaggtc cgagtcgatg cggaaccaag
     6901 ccggcggata ggactcctcg acgggcgcct cgggcgcggc gggcttggct tcgggcaccg
     6961 ccggttcggc cgtagtgggc tccggcgcag cgggcggcgt ggccgcagcc ggggcggctc
     7021 ctggtgctgg catgggcgcc gtatcggggg cgggagatgc gggagcggcg ggtgcgggag
     7081 cggccggtgc gggcgccgtg gcatcctgtg cccaagccgt tccagacagt agagctaagc
     7141 caagcgaacc gagggagaga cttttaccga gcagggtcac gcgcatgctg atctccgaag
     7201 tgtcgcggcg cgcagccatt tctgtgcgcc aagcggcccc gaggtggggc aggcagcgca
     7261 ttgctatccc tcgcggtgtt gcgatcgaat gtctcaattg tttcgcgaca tcgatcgtaa
     7321 aaactcgtcg cgaaatccaa gtgaaacatg cgatccggca tgttccaatg cgagaacgtg
     7381 gtgttctgcg gaagaattgc ctctgccggg catcgatacc gggccgggga ggcccagctt
     7441 cgatccgtgc gctggcaatg gtgcggcgct tgcgcggtgg ctcgggagca gcggcgcgcg
     7501 ccggct
//
LOCUS       DASTKU010000576        21868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_112727, whole genome shotgun sequence.
ACCESSION   DASTKU010000576 DASTKU010000000
VERSION     DASTKU010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 21868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21868
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112727"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VFQ61_20005"
     CDS             <1..1145
                     /locus_tag="VFQ61_20005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956795.1"
                     /translation="LSSYHISEFVERREQLLQEILEGLDAHSGAALALIYMRNGQLKS
                     PVTLQPSEAEAIERLNSNLGGCVAALESMKGSLVLCSLVSGEHVWEFKHPTIGDAYAA
                     TLVKSPEHVGIYIEGSTTERLLNQVTCGDVGIEKAVVLSNTFFPRMLTKLRDLSHSKA
                     YKSAWLSEFGAKRELHGFLARRCSKDFLSLYVSQNPSILEQVARPGLFLSAVSEVRLA
                     KRLQEVGLLPEEQRKEFVDTVSKYALGGEDVSALDDDEVRTLFTDDEFNDLVERIELE
                     LLPRLGDVRLNMQSHYCSSTSPEEHLQPLLDVFSTLRKYFQNDHHLVRLIDDEVQLAS
                     EWVEKTETPDSGRAPRALGRVEAQASPEGIRSIFDDVDASRDEEAD"
     gene            complement(1208..1657)
                     /locus_tag="VFQ61_20010"
     CDS             complement(1208..1657)
                     /locus_tag="VFQ61_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956796.1"
                     /translation="MDLYVASQPNVSNRLPRGERNARTDCQGLTKRSNPGATTPGFEL
                     FVEAPGIENDQRQLADTIGRGKGPIGVDADPADVSGRASKCAIDGGDVTESSDAYELS
                     NVVETALARALVLAAEARRWDVVLQIAEELEARRSGTPQAQHRAKPS"
     gene            complement(1806..3536)
                     /locus_tag="VFQ61_20015"
     CDS             complement(1806..3536)
                     /locus_tag="VFQ61_20015"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9956797.1"
                     /translation="MTGAYIGIDLGTTYSLVAALIDGQPRILPNAVGELLSPSAVHVG
                     EDGRVLVGSAARARAATHPEQTALAFKRDMGTDRTYKLGTRSFSPQELSSLVLAELKR
                     DAEVALGQPVTEAVVTVPAYFGELQRRATRDACELAGLHVERIINEPTAAALAYGLQH
                     RNRELRAVVLDLGGGTFDVTVLAIMEGVIEIHASAGDARLGGEDFVEVIAAYAREVLG
                     SRGARIESNLGLARLREASELAKRRLSSSENVELVLPSLPTTAGVIDVSVPLSRAEVD
                     ARCAPLLDRLAAPIQRALRDAGCTAKDIDEVLLVGGATRMPSVVRLAAQVFGRMPLRS
                     LPPDEAVALGAAVHAAVKAGDAAVSDVVATDIAPFSLGIASGHQVGQAVVMGLFSPII
                     ERGTVLPTSRVERFVTLSNQQRKLRIEVYQGEHSDCARNQKIGEYNLSGIPARPAGEE
                     AIDVRFSYDMNGVLDVDATIVSTGKAATFTIERTPGGLSKSELTAARERLARLKFHPR
                     EALPNLTAIARADALYAELVGSERAQLGVALAVFRAALESQDPKLIEAQRGALLQVLL
                     ELARGAERSQ"
     gene            complement(3533..4825)
                     /locus_tag="VFQ61_20020"
     CDS             complement(3533..4825)
                     /locus_tag="VFQ61_20020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET9956798.1"
                     /translation="MNPEQALVALSLTRPTSKDAVRRAYLRGVRAHPPERDPHGFQQV
                     RAAYELLKDAPWLWGSEETSREEPNPDAIASLQADGDVDATAVPAGDTPLGVPFGSTL
                     KSSEKGVAGAIAQALEPAPTPSVQIAKAAPVAADATNEASPAGESWRSLRAPVDSPSA
                     RFKVRYWQQRFGEPPDEGQAALVAWQALDTALDLFKNGEGSVAKPLLEGLSNWATRFG
                     VRSAELGPALNARWVLLRELAALDSKVQPELVRVLAKSIGAGKLEQASKAIDDAYAAD
                     VSFERRFPQLAPSLHRAVSPFVRRRSKKAEGPLKLVFSVIWALVMLARFIPDGAPVRE
                     NAVVPRSTPTRTFTRAPPSPAAMERAREEQLAASAADMDLILEAGSCAQVLVQWQVYA
                     AAVRVAPRHDQAAEDMLARVEPILQKCKEIRREDLKSP"
     gene            complement(4934..6043)
                     /locus_tag="VFQ61_20025"
     CDS             complement(4934..6043)
                     /locus_tag="VFQ61_20025"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HET9956799.1"
                     /translation="MTIFQTSRWEGARALWFGSVLATMFAFGCGGEQSSAPAKGTTES
                     SEGESEPRADIVIESEPQANATARVPRSQVPDTTGRPRVVGYLQVWRNRDPSKIDFDT
                     LTHLCLAFANPPAGDPAVKFPKENEPSIRDLVHAAHEKGVKVLASIGGGDLATAQPLF
                     DHIKNNRGDQVIRSLVELVRDYSLDGIDVDLEGDHVKSSYEPFVQALATALPETKLLT
                     AAVKTWESDDAPYFPDGALAEFNFINIMAYDDGGGMPADHSSMENANRDLRYWEDRIH
                     DASRVVLGVPFYAHCWGQGCPRGLLSYAEALAFDSGAALFDRITTDTYDLSLNRPQTI
                     ADKASLAKTKGGIMIWELGQDSADGALFSAVRAAQ"
     gene            complement(6240..6770)
                     /locus_tag="VFQ61_20030"
     CDS             complement(6240..6770)
                     /locus_tag="VFQ61_20030"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9956800.1"
                     /translation="MRNVAYYVATTLDGFICHHDGSLDGFLMEGEHVKDFMDSLQAFD
                     TVLMGRATYDLGRKFGVTSPYPMMRQYVFSRTLKESPDPAVELVSQDAVAVVRELKSQ
                     TGKDIWLCGGAELAAVLFAEGLVDEIIVKTNPVVFGSGKSLFTGPIRQAALELVDNKV
                     FSNGVVVLRYRVKPQT"
     gene            complement(6841..7482)
                     /locus_tag="VFQ61_20035"
     CDS             complement(6841..7482)
                     /locus_tag="VFQ61_20035"
                     /inference="COORDINATES: protein motif:HMM:NF012280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HET9956801.1"
                     /translation="MSSACTGNPVPSPNTPALEPAGTTSPSTPVSSASGSPGPASTAP
                     APSKSGPAGAMCGGLAGFGCAAGLYCAFSIEAHCGAADQTGVCKAIPEMCTEQHDPVC
                     SCSDKTYPNECYAARDGVSVAYLGECKAAQTLAEGQLCGTRGVQGECGAELYCKYKSA
                     CGATDTGGTCVPRPRMCTKIYKPVCGCDGKTYPSECVAASQGIAVQHDRECKP"
     gene            complement(7626..8129)
                     /locus_tag="VFQ61_20040"
     CDS             complement(7626..8129)
                     /locus_tag="VFQ61_20040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956802.1"
                     /translation="MTSLLGAPTVPTLALFLPTILLGAVLACGAGSSSHPAKPLGAPE
                     VVHGVASSSAGKPRAPGASPAESATVPESAGSSTPSEPATPAEKTTVELAVRSWHCFS
                     WVHRRDFATQCYETRDECTAAYRKSPHRDKVACEVKSGVVSCWEPKAAPGAPPPERIC
                     IWERETH"
     gene            complement(8126..9088)
                     /locus_tag="VFQ61_20045"
     CDS             complement(8126..9088)
                     /locus_tag="VFQ61_20045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956803.1"
                     /translation="MANPPDKTPKGTLTRDLLLKIRGTAGYKGLDASARALAESIIRL
                     AISKNQTEATYYLQHLLALLETPYQAPNAPSNKGRSLKSMQEAETDASIAVAKNRKPS
                     ASFDDWDQSEEFLCNHLDRRWEYRFTSEGSGRKVFRIDGSKASPRFVKVKVRLIGDPL
                     YIAKLRSLEDLIEKLAKRPGYILDLEFVDLPGYDVLTVNANSELWADAGNWAGGAETM
                     AHELHHALGLDDRYDYITSHARNEHMPTATRMHWFLVQMRKGRDPRRSASMMGDGGVL
                     LSEDICAIGGPLKRPARLKCEAAQSFFDPPGLPKLPRNHMIHRE"
     gene            9689..10207
                     /locus_tag="VFQ61_20050"
     CDS             9689..10207
                     /locus_tag="VFQ61_20050"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HET9956804.1"
                     /translation="MSAIGGCVAIAAVLSVSEYFVGAGAAGLLVASMGASAVLLFSSP
                     HGPLSQPWPMLGGHLVSGAVGVACARFIETRSLAAALAVGLSIALMLMLRCVHPPGGA
                     TALSAVIGSQSVKALGFTYLLTPVLLNAVITLGVAVAFNGLFGWRRYPAYLAARLDRT
                     PGDAPGDSRVQA"
     gene            complement(10466..14299)
                     /locus_tag="VFQ61_20055"
     CDS             complement(10466..14299)
                     /locus_tag="VFQ61_20055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HET9956805.1"
                     /translation="MQNAPKSTPTRQFAGLVWAPVVEGQLDTLRALLEQITRETQDGM
                     RGRWAASPLIPFSKLQTVHYARFVLIEREPGLGPELIFSTNYDGPEGEESCVESRALA
                     LHIDELVREAGPGLERVFSCCRGYREGRLATYLRRHQRKAATFYVGSSGRSRNQILWE
                     AELRRRVDAILDARDFAKTTPEQVRADVAEALGQERVVVPPFPPQPDLSSKLRRFQLG
                     AGLAAVLVLLGSGWWLGPLGPIGILLVLGLAVLRFRQLELRDPQFQPEHSRATLRQYE
                     TASATENEFLQNQLTHYVRVKPGLIRALTIRLVFFVLQRVARYTYNKGKLGDIPSIHF
                     ARWVLLPQRGVLFLSNFDSSWQSYLGDFIDQASSGLTAVWSNTEDYPHATWLLRAGSR
                     DAARFLAWTRHNQRPTQVWYSAYPGLSIVNVNANTEIRRGLATPACMDAATWLFRLRN
                     VDRLGADQQYSEEQVKEPPLPLADIQGIVLWGYGHMPCARFLMLRVERSASEARAWLS
                     ALPLSSAAAETKTAQPPEPLVNVAFTHRGLQALGVDAGLCERFSIPFVQGSEHPDRAR
                     VNGDLGLDAPEHWEWGGPLNPVHLVLMVYASTDPSAEEFADQFQHAAEQAGLTCVARL
                     EGATLPDRKEHFGFRDGIAQPVVQGSGRAEPLGNTVAAGEFLLGHKDGYGNISYSPVS
                     SAGFAFGMHGSYLVFRQLEQDVVGFWRYCAERAPDRPVRLASKMVGRWPSGATLVRHP
                     DGDPKDVRFRDEDDFTYLGTDEDNDRYGARCPFGAHIRRTNPRDWQLGTTRAGSLELS
                     NLHRILRRSRPYGPCLESTMDAEALVASALQPDADAPRVNRGLQFLCFNSNIERQFEF
                     IQQQWSNNPKFAGQNSDVDPLIGARGEPGRLGIDNPAFTLQSNVKAGIGPRALQLRNF
                     VRLRGSAYFFMPSIPAVRMLAEDASKASTAERFERIPRDEQLHIENLIETLREKLNRD
                     YITGETLRDAHPKMHGLVRATLRVERELEPRLRVGLFAHPAEYPAWIRFSNASEHVQD
                     DAARDIRGAALKLMGVPGSKLLEGAEDLTTHDFLLVTHDTFVTRDVAGFDELMKAVAA
                     GTLPRLGLLARRPSVAWRVWQSFRHHQNLLDTTFFSAVPYLFGDTAVKYSLRPRKLAG
                     VERAQLPRSATHLRDALKADLSRGPAVFEFLVQFYVSEARTPIEDPTVRWDERITPFH
                     HVATLEIAAQEFDTEPRRAFGENLVFNPWRCLPEHRPLGGIQRARRQVYRALSAFRRA
                     RNAAVSKEPTVESNP"
     gene            complement(14460..15248)
                     /locus_tag="VFQ61_20060"
     CDS             complement(14460..15248)
                     /locus_tag="VFQ61_20060"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9956806.1"
                     /translation="MSTETYSLFSGSLRATVELRGSAHGRTYFLLHGGAGPASMAPLL
                     DRLARDHRVVAPILPGFSGTDRPAHIRTVSDLALTYLALLDTMQAHEVVLVGNSFGGW
                     LAAEIALRATQRIVGVVFLNTVGIHTGSPARTIVDPTAVPLARRAALSFHDPAQFAGF
                     LGAPEVARYAQENQRALRTYAGEPFMHDPSLYERLAGMRTSSLVIWGESDRIVDMEYG
                     RRFASVMPGSSFVSLPGAGHFPQIEQSEEVARQIQLFGRERAQG"
     gene            15347..15769
                     /locus_tag="VFQ61_20065"
     CDS             15347..15769
                     /locus_tag="VFQ61_20065"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET9956807.1"
                     /translation="MSTEPEHLAWLVKRLQHRHHRALDTRLQQSLDVSLVQWNALREI
                     DRHPGDSQRSLAEHTFNSVQAFGTLVARLMERGLVTRVDGQGRATKHELTPKGEALLR
                     AGQQILQEITRTSFAPLSKTERVELRRLLELVLKDGAG"
     gene            complement(15787..16143)
                     /locus_tag="VFQ61_20070"
     CDS             complement(15787..16143)
                     /locus_tag="VFQ61_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956808.1"
                     /translation="MAKRLFVLKQLAMVPVGGEVEIRFFQKPKRSSWDGSLKDVRGEP
                     VQHEAVIRDLATGVIFGMDWHFQLGLDSPTQRDACPELVLVETVRGRVTSCQVVCSWG
                     SGEQRIETWLQISALP"
     gene            complement(16201..17904)
                     /locus_tag="VFQ61_20075"
     CDS             complement(16201..17904)
                     /locus_tag="VFQ61_20075"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9956809.1"
                     /translation="MAEGRASEGAPLEESASGAKATQAEAAKADAELARGWAQAASGP
                     PPGQSGSRIRAVRVVSRQPPAAPIERQTYASAALLTGKERPVRVLARRLAHARTQFDV
                     VWLQRCSQMPELALEDADYGEPIQRATAFLLSSLGNAQFELAELHCAELFVDLRELCV
                     AMASVLRIIEAFELAALQVAQDADSELAVRELGRFLTHVAVDVFANRNEGSITDSRVQ
                     VCATSEPSGEQADARPRATGELGLIGESPGIRALRAQIRDIANAPGSVLITGQSGTGK
                     ELVARAIHESAGARQRPFLPVNCAALPRELIESELFGHERGAFTGSRTSAPGMLRAAG
                     DGTLFLDEITEMPESLQPKLLRALEQRSVRPVGGLREVPIRARIIAATNGDPEHAVAT
                     GRMRADLFYRLCVHCVEVPALHERRGDIPLLIRHFVSELAKQGHTVPREFAPETLRRL
                     EDYGWPGNVRELRNVVEHCCATAKNGRVHAEHLPRQFLKRAAARSISASEPRSREPAP
                     LAAESSFPPLESVERQHIEQALTLAAGNKARAARLLGLSRHQLYLRLERLGRGDGPDI
                     E"
     gene            complement(18096..18917)
                     /locus_tag="VFQ61_20080"
     CDS             complement(18096..18917)
                     /locus_tag="VFQ61_20080"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HET9956810.1"
                     /translation="MFSTISGRMITILTAFALLLATGVFASRVILQQQADDGLLVNLA
                     GRQRMLTQKMTKESAQLVNAAHEHNEASVASLRDQLQNTMRVFEATLIALKDGGPAPL
                     NLDMTRVRQAPAAATPEISKQLGAVDALWRPFKKQMGTLIQSNGADASAAAAVMSGNL
                     QLMGEMDAAVTLMQQDSERKVNVLFWVQYAALGLGLGLVGIGVWVARSTIARPIIELA
                     AAARSMSTGNLNVELNMEGTDEVKELGESFDRMRASMVAAMGGGLAATGTDDDDL"
     gene            complement(18895..20682)
                     /locus_tag="VFQ61_20085"
     CDS             complement(18895..20682)
                     /locus_tag="VFQ61_20085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9956811.1"
                     /translation="MESVLPIPDEGRLEIGSAVTHYVVEGFLGQGAEGNVYLARDSVL
                     GRLVALKTLRAGTVLETRGVEEARILSALEHPNIVRVYQARRYQGAWVVVFEYLSGGS
                     VHALVRRVGALSPRQALEYTAQAGAGLAFAHEAGFLHRDVKPHNMLISRAGEVKLADF
                     GLALDIRTERRSGGNPVGTPAFLAPEIWAGDLGSAASDVFSLGACLFFLLTGRLPFVA
                     TDAEQLRRAHLELEPKLPSGLPGPVRDLVLTMMAKAPEQRPASAALPEMLRSLARNPQ
                     RRITTESFLPVVNACDPFVDGGSERALSRVIREGREQADLSELLSALRSRPLGLELCA
                     PEPADALLLLGVAREEVPDRFQPLARLTFSKAQSSLRSVIERKLGVKAALSLRQACEG
                     ILQQGPRDTALRPVLELYAPRALNDAQREEIAQLAGLASPLGVTCVLIRGRVSDDGIE
                     GFRRVAAFESQEAERALDERLQRWLAIATGGRFSFTRDARLLISQVCREQSRWWARLA
                     QDSLLISAAAGTRVVTTWAVMGACTQTTPWHSVEDVPAALRKKPFCWPTPELAARLAV
                     LRAADARRSAPMTPTLEKGQLPHVFDHQR"
     gene            complement(20734..21417)
                     /locus_tag="VFQ61_20090"
     CDS             complement(20734..21417)
                     /locus_tag="VFQ61_20090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956812.1"
                     /translation="MEALASLSKADGVLGVVIFDEDGTCLSNQLPSPYEPILMFEIVK
                     RLSVGFDAFSSLDPSPIASFSVECEDGSLLLRRLEKHWVVALTHPDANMSMINVALNV
                     VVLNLNRGFPGSGKQPVSRSSTDSMLGHSSTSISLSHGGQDLEIPPDAVDRSFVQQLL
                     VIYTDYLGPAARAVFKQQLAALGVTSRTLRRAQVNDLIARLVTKIPVAQRQKEFTAAA
                     RKHQDKLPP"
     gene            complement(21420..>21868)
                     /locus_tag="VFQ61_20095"
     CDS             complement(21420..>21868)
                     /locus_tag="VFQ61_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956813.1"
                     /translation="VNGSSSNSTSGVSAPISVAAPRPAEPNQEKNVTPTKIEPKSDKA
                     DLHQLLLIDSSLKAAARADRQGGVLETAGEMDAETACAVALMASHEVSEAAAEIGLGR
                     PAAWHVSLGNSTWYIVHCREELVVTFGSVTKNPASTLSKVAKSCGV"
BASE COUNT         4069 a         7029 c         6626 g         4144 t
ORIGIN      
        1 atttgagcag ctaccacatc agcgagtttg ttgagcgtcg cgagcagctc ctccaagaga
       61 ttcttgaagg gcttgacgct cacagtggag ctgcgctcgc gttgatctac atgcgaaacg
      121 ggcaactcaa aagccccgtg acgctccagc cgtcggaggc cgaagcgatc gagcggctaa
      181 acagcaacct tggcgggtgt gtcgccgctc tcgagtcaat gaagggcagc ctcgtgctat
      241 gctccctcgt atctggtgaa cacgtctggg agttcaagca cccgacgatc ggcgacgcat
      301 atgcggcaac cctggttaag agccccgagc atgtgggaat ctatatcgag ggcagcacaa
      361 cggagcgact gctgaatcag gtcacgtgcg gcgacgttgg catcgagaag gccgtagtcc
      421 tgtcaaatac cttttttccg aggatgctca cgaaactgcg ggacctttcc cacagtaagg
      481 cttacaagag cgcgtggctc tcggagtttg gcgcaaagcg ggagctacac ggattcctgg
      541 cgcgccgctg ctcaaaggac ttcctatcgc tctacgtttc gcagaatccg tccatcctcg
      601 agcaggtggc gcgtccgggg ctatttctca gcgccgtatc cgaggtgcgt ctcgcaaagc
      661 ggctgcagga agtcggactg cttccggagg agcaacggaa ggagttcgtg gatactgtca
      721 gcaagtatgc gctcggcggg gaagacgtct ctgcgctaga tgacgacgag gtccgaacgc
      781 ttttcacgga cgacgagttc aacgacttgg tagaacgaat agagcttgaa cttcttccgc
      841 ggcttgggga cgtccggctt aatatgcagt cgcattattg ttcaagcaca tctccagagg
      901 agcatctgca gccactcttg gacgtgttca gcaccctaag gaagtacttt cagaatgatc
      961 atcatttagt ccgcttgatc gacgacgaag tgcaactggc aagcgaatgg gtagaaaaaa
     1021 cagagactcc cgattctggg agagcaccac gggcacttgg cagggtcgag gcgcaagcga
     1081 gccccgaggg catcagaagc atctttgatg acgttgacgc tagcagggac gaagaagctg
     1141 actgagccag cagcgtcggg cttgcgctag aacgtcgaac aatgtcggta ggctcgccaa
     1201 aggcggtcta gctaggtttg gcacgatgct gtgcttgcgg cgtgcccgat cgcctcgcct
     1261 ccagctcttc ggcgatctgc agcaccacgt cccaccgcct tgcctccgcc gccagaacca
     1321 gcgccctggc taacgcggtc tctaccacgt tcgacagctc ataggcgtct gacgattccg
     1381 tcacatcacc accatcgatc gcgcacttag atgcacgacc tgacacgtcg gccggatccg
     1441 catcgacgcc gatcgggccc tttccgcgcc cgattgtgtc cgccagctgc ctctggtcgt
     1501 tttcgattcc cggcgcctcc acaaacagtt cgaacccggg agttgttgct cccgggttcg
     1561 agcgctttgt gagtccctgg cagtcggtcc gcgcgttgcg ctcgccgcga ggtagacggt
     1621 tcgagacgtt tggctggctc gcgacataga ggtccataga ggtccccggc tttcgtagaa
     1681 gcatgagccg caaaccttcg accccgatca actcgcgctc gggttcttgc ggtgacgccg
     1741 gctcgcgaac tctaaggcga gagtctattc gtcgatggct atgtgaatcg ccacaaccca
     1801 tcgggtcact gagaccgctc ggcgcctcta gcaagttcga ggagcacctg gagaagagcc
     1861 ccgcgttgcg cctcgatgag tttcgggtcc tgtgactcga gcgcggctcg gaagacggcc
     1921 agggcgaccc ctagctgtgc tcgctcgcta cccacgagct ccgcgtagag ggcgtccgct
     1981 cgcgcgatcg ccgtgaggtt cggaagcgct tcgcgaggat gaaacttcaa acgggccagg
     2041 cgttcgcggg ccgccgtgag ctccgacttg gagagcccgc cgggcgtgcg ctcgattgtg
     2101 aatgtcgcgg ctttgccagt cgagacgatc gttgcgtcga cgtcgagcac gccgttcatg
     2161 tcgtagctga agcgcacgtc gatcgcttct tctccggccg ggcgtgcggg aatgcccgac
     2221 aggttgtact caccgatctt ttgattgcgg gcgcaatcgg agtgttcacc ttgatagacc
     2281 tcgatcctga gctttcgttg ctggttcgag agcgtgacga accgctccac acggcttgtc
     2341 gggagaacgg ttcctcgctc gatgatggga gagaacagcc ccattaccac cgcctgaccc
     2401 acttgatgtc cgctggcgat gcccaagctg aacggtgcga tgtccgtcgc tacgacgtcg
     2461 ctcaccgctg cgtctccggc cttgacggcc gcgtgcaccg ccgctccgag cgctaccgct
     2521 tcatccggag gcagcgagcg cagcggcata cgaccgaaca cttgcgcggc aaggcggact
     2581 acgctcggca tgcgggtcgc gccgccgact agcagcacct cgtcgatgtc cttcgccgta
     2641 cagcccgcgt cccgtaacgc gcgctggatc ggggcggcga gtcgatctag gagtggcgcg
     2701 cagcgagcgt cgacttcggc gcgcgacagg gggaccgaga catcaatcac acccgccgtg
     2761 gtgggtaagc tgggcagtac gagctccacg ttctcgctcg acgagagccg tcgctttgcg
     2821 agctcactcg cctcacggag ccgtgccaat cccaggttcg attcgatgcg cgcgccgcgt
     2881 gagccgagca cctcacgtgc gtacgcagcg atcacttcca cgaagtcctc cccgcccagg
     2941 cgtgcatctc ccgcagaggc atggatttcg atgacgcctt ccataatcgc aagcacggtc
     3001 acgtcgaatg tcccacctcc gagatcgaga accacggccc gaagttctcg attccgatgc
     3061 tgaaggccgt aggccaaagc ggctgcggtc ggctcgttga tgattcgctc gacgtgcagt
     3121 cccgctaatt cgcaggcgtc acgggtcgcc cggcgctgga gctctccgaa gtaggccggc
     3181 acggtgacga cggcttccgt gaccggctgt cccagcgcca cttccgcgtc gcgcttgagc
     3241 tccgcgagca ccagcgagga cagctcttgg ggcgagaacg agcgcgtgcc gagcttgtat
     3301 gtccggtcag ttcccatgtc ccgcttgaac gcgagggccg tctgctctgg atgggtcgcg
     3361 gcgcgcgcgc gggccgcgct tccgaccaac acccgcccgt cttcccctac gtgcaccgcg
     3421 ctcggactca gtagttcacc gactgcgttc gggagaattc gcggctgacc gtcgatgagc
     3481 gccgccacga gtgagtaggt ggtcccaagg tcaatcccga tgtacgcacc agtcatggac
     3541 tcttcaggtc ctcacgtcga atctctttac acttttgaag aataggctcc acccgtgcga
     3601 gcatgtcttc cgcagcttgg tcgtgtcgag gggcgaccct aaccgctgcg gcatagacct
     3661 gccattgcac cagcacctga gcacagctgc cggcttccag gatcaggtcc atgtccgcgg
     3721 ccgaggccgc cagttgctcc tcgcgcgcgc gttccatcgc ggcaggcgac ggcggcgcgc
     3781 gagtgaacgt gcgcgttggc gtcgacctgg gtacgaccgc gttctctcgc accggagccc
     3841 cgtccgggat gaacctagcc agcataacga gtgcccagat gacggagaag accaacttga
     3901 gaggtccttc ggccttcttc gaccggcgtc gcacgaatgg cgacaccgcg cgatgcaacg
     3961 aaggtgcaag ctgcggaaac cggcgctcga aactgacgtc cgccgcatag gcgtcgtcga
     4021 tggctttgga agcctgctcg agcttcccgg ccccgattga tttcgccagc acgcgcacta
     4081 gctctggttg tactttggaa tcgagagctg ccagttcccg tagcagcacc cagcgggcgt
     4141 tcagcgcggg cccgagttcc gcagatcgca ccccgaatcg cgtggcccag ttgctcaatc
     4201 cctccaagag gggtttagca acgctcccct cgccgttctt gaagagatcg agcgcggtat
     4261 ccagggcttg ccacgcgacc agcgccgcct gtccctcgtc gggtggttcc ccgaaccgct
     4321 gttgccaata cctcacctta aagcgagcgg aaggagaatc gacgggcgct cgaagggagc
     4381 gccaactctc gcctgcgggc gaggcctcgt tcgttgcgtc ggcagcgacc ggagctgcct
     4441 tcgcaatctg cactgacgga gtgggggcgg gctcgagagc ttgcgcgatg gctccggcga
     4501 cgcccttctc gctcgatttc aacgtcgacc cgaacggaac gccgagaggc gtatcgcctg
     4561 cagggactgc agtcgcatcc acgtccccgt cggcctgaag tgacgcgatt gcgtctgggt
     4621 tgggctcctc gcgggaggtc tcctcggatc cccataacca cggcgcgtct tttagcagct
     4681 cgtacgcggc acgaacttgc tgaaacccat gcggatcccg ctccggcgga tgcgcgcgaa
     4741 cgcctcgcaa gtacgctcgg cgaactgcgt ctttcgaggt cggacgggtc agcgagagag
     4801 caactagagc ctgctcgggg ttcattgcga aatgaacccc gagattaacc tcgggcggtt
     4861 agaaacgaaa gagtctgcac gggaggtcgc cccttgctcg tcaatttccg tcccgtatcg
     4921 gtacgctcga ctactactgc gccgctcgca ccgctgagaa gagcgcgcca tccgccgagt
     4981 cctggcctag ctcccagatc atgattccgc ccttggtctt ggcgaggctg gccttgtccg
     5041 caatcgtctg cggtcggttc agggacaggt catatgtgtc ggtcgtgatc cgatcgaaca
     5101 gtgccgctcc cgaatcgaac gctagggcct cggcatacga gagcaatccc ctgggacagc
     5161 cctggcccca acaatgggca tagaacggca cccccaaaac gacgcggctt gcatcgtgta
     5221 tgcgatcttc ccagtagcgc aagtcacgat tggcgttctc catgctggaa tgatcggcgg
     5281 gcataccgcc gccgtcatca taggccatga tgttgatgaa gttgaactcg gcgagcgccc
     5341 catccggaaa gtaaggagca tcatccgatt cccaagtctt cactgcggcc gtcaacagtt
     5401 ttgtctcggg caaggccgtc gccagtgctt gcacgaacgg ttcgtaactc gattttacgt
     5461 gatcgccttc gagatcaacg tcgatgccat cgagcgagta gtctcgaacc agctcaacga
     5521 gagatcgaat cacctggtcg ccgcgattgt tcttgatgtg atcgaacagg ggctgcgcgg
     5581 tcgcgaggtc accaccaccg atggaggcca gtaccttcac gcccttctcg tgcgccgcat
     5641 ggaccaagtc ccggatgctc ggctcgttct ccttcggaaa cttcaccgcc gggtcgcctg
     5701 ccggggggtt cgcgaacgcc aaacacaagt gtgtgagcgt gtcgaaatcg atcttggagg
     5761 ggtctcgatt tctccagact tgaaggtaac ccacgacgcg aggcctgccc gtggtatccg
     5821 gaacttggct cctcggcact cgcgcggtgg cgttggcttg cggctcactc tcgataacga
     5881 tatctgctcg aggctccgac tcgccttctg agctctcggt cgttcccttg gctggcgcgg
     5941 agctttgctc gccgccgcag ccgaaggcga acatcgtcgc caacacggaa ccaaaccaca
     6001 gcgcgcgagc gccttcccat cgactcgtct gaaagatcgt cacgatacac ctcgaccaac
     6061 tttggaaaag acgatcacac gccgatgtcg ctcatcgacg tgctcatctg aatcccaccg
     6121 tgttggtccc gctcaaaacg ggtgattctt cgaggtgctt tgccggtcgc ggctcgccgc
     6181 ttgtaacaga gtgcggaccc gccgcgcgcg gcgagagggc cggttcgcgc gcagcctctt
     6241 caagtctgcg gtttcactcg ataccgcagc accacgacac cattactaaa caccttattg
     6301 tccaccagct ccaacgcagc ctgccgaatt ggccccgtaa agagcgactt cccggaacca
     6361 aaaactactg ggttggtctt gacgataatc tcgtcgacta agccctcggc aaacaacacg
     6421 gccgccagct cagcgccccc acacagccaa atgtctttgc cggtttgact tttcaattcg
     6481 cgaaccacgg ccacggcgtc ctgcgatacc aactcgacgg caggatccgg gctctccttc
     6541 aacgtccgcg agaagacgta ctgcctcatc atcgggtagg ggctcgtcac gccgaacttg
     6601 cgtcctaaat cgtaggtcgc acgtcccatc aacaccgtat cgaaggcctg cagcgagtcc
     6661 atgaagtcct tgacgtgctc accttccatc aagaacccat ccaagctgcc atcgtggtgg
     6721 caaatgaaac catccagcgt agtggctacg tagtacgcga cattgcgcat gattcctact
     6781 gatcttcacg gttagctggc gaccgtcaag atccattccg atcgtcgccc gtccgttcgc
     6841 ttacggcttg cactcccggt catgctgcac ggcaatgccc tgactcgcgg caacgcattc
     6901 gctcgggtag gtcttaccgt cgcagccaca aacgggcttg tatatcttgg tacacatgcg
     6961 gggtcgcgga acgcaggttc ctccggtgtc tgtcgcaccg cacgcgctct tgtatttgca
     7021 atagagctct gcgccgcatt cgccctgcac tccgcgggta ccgcagagct gcccttcggc
     7081 gagggtctgg gctgccttgc attcgcccaa gtacgcgacc gaaacgccgt cgcgcgcggc
     7141 ataacattcg ttcgggtaag tcttatcgga gcaggaacat acgggatcgt gctgctcggt
     7201 acacatctcg ggaatggcct tgcaaactcc agtctggtca gcggccccgc agtgcgcctc
     7261 gatggagaag gcacagtaga ggccggcagc gcaaccaaag ccggccaatc ctccacacat
     7321 tgctccggca ggccccgact tcgacggcgc gggcgcggta gaagcaggac cgggcgagcc
     7381 cgacgccgaa ctgacgggag tgctcgggct ggtggtgccg gcaggctcca gtgccggcgt
     7441 gttgggcgac ggaacgggat tgcccgtaca ggcagaggac atcgacagag ttagcgccgc
     7501 ccaagtgatc cggatggcgg gcgaacgtct cgcaagttga gttggcatgg tgctccgccg
     7561 cacaccatac cggactcacc gttcgcgcgc acccgtcccc accggcctcg caggctggcc
     7621 cgaatctagt gcgtctcacg ctcccaaatg cagatgcgct caggaggcgg cgcgcccggc
     7681 gcggctttgg gctcccagca agaaaccact ccgctcttca cctcgcatgc aaccttgtcg
     7741 cggtgcggac tcttccgata ggccgcggtg cactcgtcgc gagtttcgta gcattgagtc
     7801 gcaaagtccc ggcgatgaac ccacgaaaag caatgccagc ttcgcaccgc gagctcgacc
     7861 gtggtctttt cggcgggcgt cgcgggttca ctcggcgtcg acgaacctgc ggattctggc
     7921 accgtggcag actctgcggg gctcgcgccg ggcgcgcgag gcttccccgc cgaagacgat
     7981 gcaaccccgt gcacgacttc gggcgccccg agcggcttgg ctgggtgcga ggagctccct
     8041 gcaccacaag cgagcaccgc cccaagcaga atcgttggca gaaacagggc cagagtcgga
     8101 accgtgggcg ccccgagcaa gctcgtcact cgcgatggat catgtgattt cggggcagct
     8161 tggggaggcc gggaggatcg aagaacgact gcgccgcctc gcacttcaaa cgggcgggtc
     8221 tcttcagtgg cccgccaatc gcacagatat cttcggagag caaaaccccg ccatcgccca
     8281 tcatactcgc agaccggcgc ggatccctgc ccttgcgcat ttgcactaga aaccaatgca
     8341 tcctcgtcgc ggtgggcatg tgttcgttgc gagcatgaga cgtaatgtaa tcatatcgat
     8401 cgtccaagcc caaggcgtgg tgaagttcat gcgccatcgt ctccgcgcca cccgcccaat
     8461 tcccggcgtc ggcccaaagt tccgaattgg cgttgacggt aagcacgtcg tacccgggca
     8521 ggtccacgaa ctcgagatcg aggatgtatc ctggccgctt cgcaagcttc tcgatcaggt
     8581 cttcgagaga gcgaagctta gcaatgtaaa gcggatcacc gatgagcctc accttcacct
     8641 tgacgaaacg cggtgatgct ttgctgccat cgatccgaaa gacttttctt cctgagcctt
     8701 cgctcgtgaa gcggtattcc cagcgccgat cgaggtgatt gcagaggaac tcctcggact
     8761 gatcccagtc gtcgaaggac gccgaaggtt tgcggttctt tgccaccgcg atcgaagcgt
     8821 ccgtttcggc ctcctgcatg gatttcaagc tccgaccctt attggaaggc gcgttggggg
     8881 cctggtacgg cgtctccagc aaagcgagca agtgctggag gtagtaggtg gcctcggtct
     8941 ggttcttgga aatcgcgagc cgaatgatac tctccgccaa cgctcgcgcg ctcgcgtcca
     9001 accccttgta tccagctgtt ccgcgaatct tcaataacaa gtcgcgagtc agggttccct
     9061 tgggtgtctt gtccggcgga ttcgccatgc ttgtttgctc ccgcgagctc gtcgcgctct
     9121 tggacggatt cggcaccgtg ccgaaccgct gaagttccgt tcgctgccca cgaggcaact
     9181 acacctgcct cgagcaccat acgcgatcag ggggcgtgat ctgcctagaa tttctgctca
     9241 aatcgcgtga ttctcagcgc aatagcctac cagcggccga ttctagaggt tggcatttgc
     9301 gctgttcacc cggaccaatg atgtcgcggg ggcattcgcg agtaaacgcg cgctcagcgc
     9361 ggaacacgat gcgcaagatt cgatggggat ggccgatggt ctgaagaccc ggtcgcgatc
     9421 gctaccgttc gtggccttcg atgttccgtc gttggccggg agtgatcggt ctcaagccgg
     9481 aacagcttgc gtctaccagc ccgaaggcca acgaaacacg ctcgaaacac tattgcgaag
     9541 ccggatcccg tcctgctatg agcgcacccc tcaccgcgaa agctcgcgcc tttcccgctt
     9601 agcaggtcat ctagtgtccc gttctctcgc gttcatttgg agttttctgg gggtgccagc
     9661 tgggcaggcg cgccggaagg aagtcattgt gtcggccatt ggaggctgcg tcgcgatcgc
     9721 cgccgtactg agcgtgagcg agtacttcgt cggtgcggga gccgcgggtc tgctcgtggc
     9781 ttcgatggga gcgagcgcgg tgctcttgtt ttcttcaccg cacgggccgc tttcgcagcc
     9841 ttggcccatg ctcggcgggc acttggtttc cggtgccgtc ggtgttgcct gcgctcgatt
     9901 catcgaaacc cgctctttgg ctgcggcgct ggcggtgggg ctttcgatcg ccctcatgct
     9961 gatgttgcgc tgtgtgcatc caccgggagg cgccaccgct ctttcggcag tgatcggctc
    10021 gcagtcggtg aaagccctcg ggttcacgta cctcctcacc ccggttctcc tcaacgccgt
    10081 aatcaccctc ggggtagcgg tcgctttcaa cggcctgttc ggctggcgcc gctaccccgc
    10141 gtacttggcc gcgcgacttg accgcactcc cggagatgct ccgggtgact cacgcgtcca
    10201 agcctgacag caaccccgta cagaccttcg gaatgccgga ggtctgtaca tcggacagtc
    10261 tgggtaaccc cacgccgacg aaggcgaact tcggctgggg ctggcagctc gggtcgtcgc
    10321 tcgtactgca tggcgcccgc agcagttcgg aatcgaggcc ttggcactac ccctgctccg
    10381 cctcgggtcg cggccttgat ctgggttcgg cgcgtggcgc cttcggctgg tgagcctccc
    10441 tcgactgagc gtgtactctg gagtctcacg ggttcgactc cacggttggc tctttgctga
    10501 ccgccgcgtt gcgagcgcgc cggaaggcgg acaaggcccg gtacacctgg cgacgggcgc
    10561 gctgaatccc tccgagcggg cgatgctctg gtaagcaccg ccagggattg aataccaggt
    10621 tttcaccgaa cgcccgccga ggctcggtat cgaattcctg agccgcaatc tctagcgtag
    10681 ctacgtggtg aaaaggtgtg attcgttcgt cccagcgcac ggtaggatcc tcgatcggag
    10741 tacgcgcctc cgacacgtag aattgcacca ggaactcgaa aaccgcgggg ccccgcgata
    10801 agtcggcttt gagcgcatct cgcaagtgag ttgccgacct tggtagttgg gcacgttcca
    10861 ccccggcgag tttacgaggc cgcaggctgt acttcacggc ggtgtcgccg aacaagtacg
    10921 gcacggcgct gaagaacgtc gtatccagca ggttctggtg atgacggaaa gattgccata
    10981 ctcgccaggc caccgacggg cgccgagcga gcaggccgag acgaggcaac gtaccggcag
    11041 caacggcttt cattagctca tcgaagccag ctacgtcgcg ggtcacgaag gtatcgtgcg
    11101 tgaccaacag gaagtcgtgg gtggtgagat cttctgcgcc ttccagcagc ttactgcccg
    11161 gcacccccat cagtttgaga gccgcacctc ggatatctcg cgccgcgtcg tcctgcacgt
    11221 gctccgaggc attggagaac cgaatccaag ccggatactc cgccgggtga gcaaacagac
    11281 ccacgcgtaa gcgaggctcg agctcacgct cgacccgcag cgtggcccgc accagaccat
    11341 gcatcttggg atgcgcatct cgcagcgttt cccccgtgat gtaatcgcga ttcagcttct
    11401 cccgcagcgt ctcgatcagg ttttcgatgt gtagctgttc gtcacgcgga atacgttcga
    11461 accgctcggc cgtagacgcc ttcgatgcgt cctccgcgag catccgcacc gcggggatcg
    11521 atggcatgaa gaagtaggcg ctaccccgca aacggacgaa gtttcggagt tgcaacgcgc
    11581 gtggaccaat ccccgccttc acgttgctct gcaaagtgaa agccggattg tcgatcccga
    11641 gccgtcccgg ctctccccgc gccccaatca gcggatcgac gtcgctattt tgacccgcaa
    11701 atttgggatt attggaccac tgctgttgaa tgaactcgaa ttggcgttcg atgttgctat
    11761 tgaagcacag gaattggagt ccccggttca cgcgaggagc atctgcgtcg ggctgcaacg
    11821 cgctcgcgac cagggcttcc gcgtccatcg tggattcgag gcagggccca tacgggcggc
    11881 ttcgacgcaa gatgcgatgc agattggata gctcgagtga gcctgcgcgc gtcgtaccca
    11941 gttgccagtc acgcgggttc gtgcgtcgaa tgtgagcgcc gaacgggcaa cgcgcgccat
    12001 atcgatcgtt gtcttcgtcc gttccaagat acgtaaaatc atcttcgtcg cgaaagcgca
    12061 cgtccttcgg atcaccgtcc gggtgacgca cgagcgtggc accgctcggc cagcgcccga
    12121 ccattttgct cgcgaggcgc acgggacgat ccggagctcg ttcggcgcag taacgccaaa
    12181 aacccaccac gtcctgctcg agttgtctaa acaccaggta actaccatgc attccgaacg
    12241 cgaatcccgc ggagctcacc gggctgtacg agatgtttcc gtagccatcc ttgtgtccga
    12301 gcaagaactc gcccgccgca acggtgttgc cgagcggctc cgcgcgccca cttccttgaa
    12361 cgactggctg cgcaatcccg tcgcggaacc caaagtgctc ctttcggtcc ggtaacgtgg
    12421 caccctcgag tcgcgctacg caggtcaatc cagcctgctc cgccgcgtgc tgaaactggt
    12481 cggcgaactc ttccgcgctg gggtccgtac tcgcgtagac catcagtacc aagtgcacgg
    12541 ggttaagggg ccctccccac tcccagtgct ccggtgcgtc taggccgaga tcgccgttta
    12601 cgcgagctcg gtcgggatgt tcggaaccct gcacgaacgg tatcgaaaag cgctcgcaca
    12661 agccggcgtc caccccgagc gcctgcagac cccggtgagt gaaagccacg ttcaccagtg
    12721 gttcgggcgg ctgagccgtc ttcgtctccg cggccgccga cgagagcggg agtgcgctca
    12781 gccaggcgcg cgcctcagaa gcggagcgtt ccacgcgcaa catcaggaaa cgtgcacacg
    12841 gcatgtggcc atagccccag agcacgatgc cttgaatatc cgccagcggc aatggcggct
    12901 ctttgacctg ctcttcgctg tactgctggt cggccccgag ccgatctaca tttcgaagtc
    12961 gaaatagcca ggtcgcggcg tccatgcagg ccggcgtcgc caagccgcgg cgaatctcgg
    13021 tgttggcatt tacattcacg atcgacagcc ccggataggc gctgtaccac acctgggttg
    13081 gccgctgatt gtgccgagtc caagccaaaa accgcgccgc gtcgcgagaa ccagcacgca
    13141 atagccaagt agcgtggggg tagtcttcgg tgttgctcca gacggcggtc aatccgctac
    13201 tcgcctggtc gatgaagtct cctaggtagc tttgccacga actatcgaag ttactgagaa
    13261 acagcacgcc gcgctggggt aggagcaccc aacgggcaaa gtgaatggag gggatgtctc
    13321 cgagcttccc tttgttgtag gtataacgcg cgactcgctg cagtacgaag aacaccagtc
    13381 ggatcgtcag ggcgcggatg agccccggtt tgacgcgcac gtagtgtgtg agctggttct
    13441 gaagaaactc attctcggtg gcggaggcgg tctcgtactg ccgcaacgtg gctcgcgagt
    13501 gctccggctg aaactgtgga tcacgaagct cgagctgccg aaaacgaagc acggcgagcc
    13561 ccagcaccaa taagattcca atcgggccga gcggaccaag ccaccatccc gagcccagca
    13621 gaaccagcac cgccgccaaa cccgccccga gctggaatct ccgcaacttg ctgctcaaat
    13681 ccggctgagg aggaaacggt ggaaccacga cccgttcctg tcccagagcc tccgctacat
    13741 ccgctctcac ctgctcggga gtggtcttcg cgaaatcacg agcgtccaga atcgcgtcca
    13801 cgcgccgccg cagctccgcc tcccacagga tctgatttcg acttcgcccc gacgatccga
    13861 cataaaacgt cgcggctttc cgctggtgcc tgcgcagata tgtggcgagc cgcccctcac
    13921 ggtagcctcg acaacaggag aacactcgct ccaggcccgg tccggcttcg cgcactagct
    13981 catcgatgtg cagcgcgaga gcgcggctct cgacgcacga ctcttccccc tctggcccat
    14041 cgtaattcgt gctgaagatg agctctggtc cgagcccagg ctcacgctcg atcaaaacga
    14101 atcgggcgta gtgaacggtc tggagtttcg agaacggaat gagtggtgag gctgcccacc
    14161 gaccacgcat tccgtcctga gtctcgcgcg taatttgctc caagagagct cgaagcgtat
    14221 ccaattgccc ctcgaccaca ggcgcccaca ccagccctgc gaattgacgt gtcggggtgc
    14281 tcttgggtgc attctgcatt cgtccgtgct tcctgaaaaa ccgtcacttt cgcacagtcc
    14341 tgctgggttc caccagtgtc catcgtcgtg gctctccgaa gcggctggaa ctgtcgaata
    14401 tcgatagacg ccgtctctcc cgatcccacg atgcaagaga tcgcactcag gctctgagct
    14461 catccctgag ctcgctcccg tccaaaaagc tgaatctggc gagcgacctc ctcagattgc
    14521 tcgatttgtg ggaagtgacc ggcgccgggg agcgacacga acgagctgcc cggcatcacc
    14581 gaggcgaaac ggcgcccgta ctccatgtct acgattcgat cgctctctcc ccaaattacg
    14641 agcgatgacg ttcgcatccc ggccaagcgc tcgtacaggg aggggtcgtg catgaagggc
    14701 tcgccggcgt acgtacgcaa cgcgcgttgg ttttcctgag cgtaccgcgc gacctcgggc
    14761 gcaccgagaa atccggcgaa ctgcgcggga tcgtgaaagg agagtgcggc gcgtcgagca
    14821 agggggaccg cggttggatc tacgatcgtc cgcgcgggtg agccggtgtg gatgcctacg
    14881 gtgttgagaa agaccacacc aacgatcctc tgggtcgcgc ggagagcaat ctctgccgcg
    14941 agccacccgc cgaacgagtt gcccaccagc accacttcgt gcgcttgcat ggtgtcgaga
    15001 agggccaaat acgtgagcgc gagatcactc accgtccgga tgtgtgccgg ccgatccgta
    15061 ccggaaaagc ctggcagaat gggcgccacg acgcggtggt ctcgcgcgag cctatcgagc
    15121 agcggcgcca tcgaagccgg gcctgcgccc ccgtgaagga gaaaataggt gcgcccatgg
    15181 gcggagcccc gaagctcgac cgtagcgcgc aaagatccgg agaaaagaga atacgtttcc
    15241 gtgctcatat cgcgaggctc ctcgactgga agatttcttg gcaaaaaccc gggccgcccg
    15301 gttcgagcgt gacttatgat caacatgttg atatacgcat gttgatatgt caaccgaacc
    15361 cgagcatctc gcctggctcg tgaagcggct gcagcaccgt caccaccggg ctttggatac
    15421 gaggctccag caatcgttgg atgtgagcct cgtgcaatgg aacgctctcc gggagatcga
    15481 tcgccacccc ggggactctc agcgcagcct cgccgagcac acgttcaact cagtgcaggc
    15541 ctttggcact ctagtggctc gattgatgga gcgcggcttg gtcacgcgcg ttgacggaca
    15601 gggccgtgcc accaagcacg aactcacgcc gaaaggcgag gctctgctgc gtgccgggca
    15661 gcagatcttg caggagatca cccggacgtc gttcgctccc ctcagcaaga ccgagcgcgt
    15721 cgagctccgc cgcttgctcg aactcgtcct gaaagacggc gcaggctagc gctcagcttg
    15781 ctggcttcag ggcagggcgg agatctgaag ccaagtttcg atgcgctgct cccccgaccc
    15841 ccagctgcac acgacttggc agctcgtcac ccggccacgc actgtttcga ccaggacgag
    15901 ttccgggcag gcgtcacgct gggttggcga gtccagcccg agctggaaat gccagtccat
    15961 gccgaagatg acgccggttg caaggtcgcg gattacggcc tcgtgctgca cgggttcgcc
    16021 acgcacgtct ttcaggctgc cgtcccagct gctgcgcttt ggtttctgaa agaaccggat
    16081 ctccacttca ccaccgacgg gcaccatcgc cagctgcttg agaacgaaca agcgttttgc
    16141 catcgcgcca gcttagagcc gttggctccg gcaaagtagc taccatctcc tgcgcacggt
    16201 ttattcgatg tcgggaccat cgccgcggcc caggcgctcg aggcgcaagt acagctgatg
    16261 ccgtgacagc ccgagcaatc ttgcggcgcg cgctttgttg cctgcggcca aggtgagcgc
    16321 ctgctcgatg tgctgccgtt cgacggactc gagtggcggg aaactggatt cagcggcgag
    16381 cggagcgggc tctcgggagc gcggctcaga agccgagatg ctgcgcgcgg ctgctcgctt
    16441 cagaaactgg cgcgggaggt gttccgcatg cacgcggccg tttttggcgg tcgcacagca
    16501 gtgctcgacg acattgcgca gttcgcggac gttgcccggc cagccatagt cctcgagcct
    16561 ccgcagggtc tcgggcgcga attcacgagg aaccgtgtga ccctgcttgg cgagttcgct
    16621 cacgaagtgc cggatcagga gcgggatgtc ccctcgcctt tcgtgcagcg cgggtacctc
    16681 gacgcagtgc acgcacaagc gatagaacag gtcggctcgc attctgcctg tcgcgacggc
    16741 gtgttcggga tcaccgttcg tagcggcgat gatgcgggcg cggatcggga cttcgcggag
    16801 cccacccacc ggtcgtaccg aacgttgctc cagggcacgc aacagcttgg gttgcaggct
    16861 ctccggcatc tccgtaattt catccaggaa cagcgttccg tcgcccgcgg cgcgcagcat
    16921 cccgggcgcg ctggtacggc tgcccgtgaa cgcacctcgc tcgtgtccga acagctcact
    16981 ctcgatgagc tcacgtggca aggcggcgca gttcacgggc aagaacggcc gctggcgcgc
    17041 gcccgccgat tcgtggatgg cgcgtgccac gagctctttg ccagtcccgc tctgcccagt
    17101 gatgagcacc gatccggggg cgttagcaat gtctcgaatc tgtgcgcgca gggcgcgtat
    17161 gcccgggctc tcaccgatca gcccgagctc gccggttgct cgcggccgag catcagcctg
    17221 ttccccagat ggctcgctcg tggcgcacac ctgcacgcgg ctgtcggtga tggacccttc
    17281 gttgcgattt gcgaatacat ccaccgcgac gtgagtcaga aagcgtccta gctctcggac
    17341 cgcgagttcg gagtccgcat cctgcgcaac ctgcaacgcg gcgagctcga acgcttctat
    17401 gatccgcaac accgaggcca tggccacgca gagttcgcgt aaatccacga atagctcagc
    17461 gcagtgcaat tcggcgagct cgaactgagc gttgccgagt gagctcagga gaaaggccgt
    17521 cgcgcgctgg atcggctcgc cgtagtccgc atcctccagc gcgagctccg gcatttgcga
    17581 gcagcgctgg agccacacga catcgaattg cgtgcgagcg tgagctagac gccgggcgag
    17641 cacgcgaacc ggccgctcct taccggtcag gagcgccgcc gaagcatacg tttgccgctc
    17701 gatgggagcc gccgggggct gccgtgagac cacccgcacc gcgcggatgc gcgaaccgct
    17761 ctgccctggc ggtgggccgc tcgcggcttg tgcccagccg cgagccaact ctgcgtctgc
    17821 ctttgcggcc tctgcctgcg tggccttggc gcctgacgcg ctttcctcga ggggagcgcc
    17881 ctcggaggcc cttccctcgg ccacgagact aggcaatcga cctggcacga cgaacccgga
    17941 gaacggacat cgcccctccg aattaagcgt ccttgtttgc ggtgcgatcg ccacgaggcc
    18001 gcgcaagctc ggagcctcgt ctcatgctga atcaactgat tcggaatcgc tcatgccgaa
    18061 tcatgagtcg tggacagacc tgttacacac atggatcaca aatcgtcatc gtccgtaccc
    18121 gtagccgcca ggccacctcc cattgccgca accatgctcg cgcgcatgcg gtcgaacgac
    18181 tcaccgagct ctttgacttc gtccgtacct tccatgttga gctctacgtt caagttaccg
    18241 gtgctcatcg atcgtgcggc cgccgcgagt tcgatgatgg gacgagcaat cgtggaacgc
    18301 gctacccaca ctccgatgcc tacgagccca agtcccaacc cgagcgctgc gtattgcacc
    18361 cagaagagca cgttcacctt tcgttcggag tcctgctgca tcagcgtaac cgccgcgtcc
    18421 atttctccca tcagctgcag attgccactc atcacggcgg cggcggcact cgcgtcggcc
    18481 ccgttggatt gaatgagcgt tcccatctgc tttttgaagg gtcgccacag agcgtcgacg
    18541 gcgccgagct gcttactgat ctctggggtg gcggccgcgg gcgcttgcct cacgcgggtc
    18601 atatccagat tcaggggcgc cggaccaccg tctttcagtg cgataagggt ggcttcgaaa
    18661 acgcgcatgg tgttctgcag ctgatcacgc aagctcgcaa ccgaagcctc gttgtgctcg
    18721 tgcgcggcgt tgaccaactg cgcgctttcc ttggtcatct tctgggtgag catgcgctgc
    18781 cgcccggcga ggttgacgag taggccgtcg tccgcctgtt gctgcaagat gacccggctg
    18841 gcgaagacgc cggtggcgag caacagagcg aacgccgtca atatggtgat cattctaccg
    18901 ctgatggtcg aaaacatggg gcagctgtcc tttctctaac gttggagtca tgggggcgga
    18961 gcggcgcgcg tcggccgcgc gaagcacggc caatcgcgcg gcgagttccg gcgtgggcca
    19021 acagaatggc ttttttctca gggccgccgg cacatcctcg accgaatgcc aaggcgtggt
    19081 ctgcgtacag gcgcccatta cggcccaggt cgtgaccact cgagtgcctg cggccgcact
    19141 gatcaacaaa ctgtcttgcg cgagacgagc ccaccaacgg ctctgctccc gacacacttg
    19201 agagattaag aggcgagcat cccgcgtgaa gctgaaccgc ccgcccgtcg cgatcgcgag
    19261 ccagcgttgc agccgctcgt ccaaggcacg ctcggcctcc tggctttcga acgcggcgac
    19321 acgccgaaat ccctcgatgc catcatccga gacccgcccg cgaatcagca cgcacgtgac
    19381 ccccaatggg ctggcgagcc ccgccaattg cgcgatctct tcgcgctgag cgtcgttcag
    19441 cgcgcgtggt gcatagagct ccaacactgg ccgtagagcg gtgtcacgcg ggccttgctg
    19501 caagatgccc tcgcacgcct ggcgtaacga gagcgccgcc ttgaccccca gcttccgctc
    19561 gatcacggac cgcaagctcg attgagcctt gctgaaggtg aggcgcgcga gcggttgaaa
    19621 tcgatcggga acctcttcgc gggccacgcc gagcagcagc aaagcgtcgg ccggttcagg
    19681 tgcgcacagc tccaaaccga ggggtcgcga gcgcaacgcg ctgagcagct cggacaaatc
    19741 agcctgctct ctgccttcgc gaatgacgcg agagagcgca cgctcggaac ctccgtctac
    19801 gaacggatcg caagcgttga cgactggtaa gaacgactcg gtcgtgatgc gtcgctgtgg
    19861 attacgcgcc agcgatcgga gcatctcggg cagcgccgcg ctcgcgggac gttgctcggg
    19921 tgccttggcc atcatcgtga gcacgagatc gcggactggc cctggtagcc ctgacggcag
    19981 cttcggctcg agctccaaat gggcgcgcct cagctgctcc gcgtccgtgg ccacgaacgg
    20041 taagcggccc gtaagcagaa agaataggca agcgcccaga ctaaacacat ccgaggccgc
    20101 gctgccgaga tctcccgccc aaatctcggg tgccaggaac gccggggttc ccaccggatt
    20161 gccacccgag cggcgctccg tgcggatatc cagcgccaaa ccaaagtcag cgagcttcac
    20221 ctcgccggcc cgggatatca gcatattgtg aggtttgaca tcccggtgca gaaagccggc
    20281 ctcgtgcgcg aacgcgagac ccgccccggc ttgcgcagtg tattcgaggg cttgtctcgg
    20341 cgaaagcgcg cccactcgac ggaccagcgc gtgcacgctg ccgcccgata ggtactcgaa
    20401 caccacgacc caggccccct gatagcgccg ggcctggtac acgcgaacga tgttcgggtg
    20461 ttcgagcgca gagagaatgc gcgcctcctc caccccgcgc gtctccagca ccgttcccgc
    20521 gcgtagcgtc ttcagcgcga cgagccgccc gagtacggag tctcgcgcca ggtaaacgtt
    20581 accttcagcg ccttgtccca gaaagccttc caccacgtag tgcgtgacgg cactaccgat
    20641 ttctagacgg ccctcgtcag gtatgggcag tacggattcc atgagctgtc accgaccgaa
    20701 cgacgtcatc acccgcatcg caggagccgg cgctcagggc ggaagcttgt cttggtgttt
    20761 gcgcgcagcg gccgtaaact ctttctggcg ctgagctacc gggatcttgg tgaccaaacg
    20821 cgcgatcagg tcgtttacct gtgcacgccg tagcgtcctc gacgtgaccc ccagcgcggc
    20881 cagctgctgc ttgaagaccg cgcgcgcggc cggccccaga tagtccgtgt aaatcaccaa
    20941 gagttgctgc acgaaactcc ggtctacggc gtcgggagga atttcgaggt cttggccacc
    21001 gtgagagaga gaaatactcg tgctcgaatg cccgagcatc gagtcggtcg agctgcggga
    21061 tacgggttgc ttgccgctgc cggggaagcc tcgattcagg ttcaagacca cgacgttcag
    21121 ggcaacgttg atcatgctca tgttcgcgtc cggatgcgtg agcgccacga cccagtgctt
    21181 ctccaagcga cgcaatagca gactgccgtc ttcgcactcc acggagaagc tcgcgatcgg
    21241 gctcggatcc agtgacgaga aggcgtcgaa accgaccgac aaacgtttga cgatctcgaa
    21301 catcaagatg ggctcgtagg gtgacggcaa ctggttggat aggcaggtcc catcctcgtc
    21361 gaagatcacc acaccgagca caccatctgc tttcgatagg ctggctagcg cttccatgtt
    21421 caaactccac agctcttggc gaccttgctc aaggtcgagg cagggttctt ggtgacactc
    21481 ccgaaggtca ccacgagttc ctcgcggcag tgcacgatgt accaggtgct attgccgagc
    21541 gagacgtgcc acgctgccgg gcggcccaag ccaatctcgg cggcggcctc ggaaacctcg
    21601 tgacttgcca tcaacgcgac ggcgcacgcc gtctcggcgt ccatctcgcc tgccgtctcc
    21661 agcacgccgc cctgccgatc ggcgcgcgcg gccgccttca gggaactatc gatcagcaag
    21721 agttgatgaa ggtctgcttt gtctgatttc ggttctatct tggtcggagt cacgttcttc
    21781 tcctgattgg gttcggcggg gcgcggggcg gctacgctga ttggcgcgct cacgcccgag
    21841 gtgctgttgg acgagctgcc gtttacga
//
LOCUS       DASTKU010000577         6624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_781269, whole genome shotgun sequence.
ACCESSION   DASTKU010000577 DASTKU010000000
VERSION     DASTKU010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781269"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2733)
                     /locus_tag="VFQ61_20100"
     CDS             complement(<1..2733)
                     /locus_tag="VFQ61_20100"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9956814.1"
                     /translation="MGFSKPVLVLWGRSAIVFCNQASLAFLGPGERSKGGSHTELLGR
                     PVRSSYAETTGTYRALLGQVLTTVNSTDGWAELGWSFRDVTRASVERSSFELECIPIR
                     DLEGGLRGTFVVWQERRTDEPATSQQLLACMTDSLRPLADPIEIQGTAAQLLGSHLNV
                     TRVMYGEVEADDEHIRFDRGYAEAGAPNLLGRFRMRDFGEHLVTALFAGQTIVIDEVA
                     VSQELTPRERSAYSGLGIAALVGVPLVKQGRLVANISVHAARARRWSQEEVSLIQETA
                     ERTWAAVQRARAEAALRQRDEEYRLLFEAMEEGYALCEIVRDEAGQTKDIRYLELNRA
                     FENLLGLPRGRFVGRLRSELLRDPMKETLLRYSHAVETSRPTRFEKYIEALDRWFDVR
                     VHPRADDRFAMIYDDITTRKKAEQTLLDDDARKTCLLAFSDRVRAAAGPLDIHAIATE
                     LLRDYFGASCFSVIYDDEAGFALVLTEMLVGDVPSLKGQYALVDFPLPSSRLREGMPL
                     VESSRSFSTIPLDKQGVQFRAANVTVPIVRSGRLLASLTVLDPRPRDWTEHDVDLVQQ
                     VSERLWIAVERGRTEAAHRESERLARRLLEQATAARAQVEAANRSKDEFLATLSHELR
                     TPLAAIQLWAGALRSGAVPSKDMPRALESITQSADSQSRLIEDLLDLSRLTSGKLALS
                     RAPHDVRRLGEAAMEMIEPIALEKSIALTIRISDEVTLAVLDGARIKQVLWNLLTNAL
                     KFTPRGGSVSLRISATDAVLEFEVKDTGEGIPPESVPRVFDKFWQADMGPTRAHLGLG
                     IGLALAKQLVELHGGRITAQSDGRGMGATFRVELPLIQDDSSVANEAESELHGRGFGA
                     AAPLESVRVLLVEDDSSTQAAMQRVLEHAGARVTAVVTAKEGLHALDSEPIDVII"
     gene            complement(3053..4330)
                     /locus_tag="VFQ61_20105"
     CDS             complement(3053..4330)
                     /locus_tag="VFQ61_20105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956815.1"
                     /translation="MYRYGLSGVMVSLTVALACRAEPDSTSGSAIGWVEEDASAASTR
                     EARGGATAEAPACSLIPGETSCVAAFPRRILHECREEDTFLASAVLANALPENQDVLN
                     IELVLAATDPRVQESPACAANFRECHPLSEPVPGYSPPIAECFTGQCMSPDIRPASSG
                     RPYVWKLLNQEGAGTMEVELPPNAALSREWRRATAVILHHDNGHGWPQAMVLYWNGFL
                     RMKTLSVPDRCFGSSFVLPPFLPVEQPATEPPRPELHPVIDRLVLGEDESGRPRLQAY
                     GAWYDLDWFRPLEVEWTLVMDLPSTDGSTQWRVSTRITRGESSISTASTGRVFDVLGI
                     GAISSMYSRPGSFDADYFEVDPGEVVGAASLLGPSGTTVNGSLQVLGPGSSMKLARSA
                     PSDHNASGPDLRLTLVSIASSGANATIETGAAP"
     gene            complement(4596..5513)
                     /locus_tag="VFQ61_20110"
     CDS             complement(4596..5513)
                     /locus_tag="VFQ61_20110"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9956816.1"
                     /translation="MKPDADVIPPARMPLSCESDLRCGAASSNGSSCCESIWLSGGTF
                     PMGFDPKEVPFPQVLQRASADHPVTVSGFYLDRFEITVGRYAAFARHAPPPPADGDGA
                     HPQIANSGWHAEWDARLDRDVSALLPSMSTGLEAVAADLPSDPNLPMTGLDWFTALAF
                     CVWDGGRLPTEAEWEFAAAGGELNRSYPWGADPSLITSLRESGPRPVGSSPETRGAFG
                     HEDLAGSAEEWTLDWYDAGFYERVGAQCRDCANLSPQLGRVVRGARDSDCCAGMDSLY
                     RSASRAQQAPGMRQSGLGARCARDPQTVR"
     gene            complement(5631..>6624)
                     /locus_tag="VFQ61_20115"
     CDS             complement(5631..>6624)
                     /locus_tag="VFQ61_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956817.1"
                     /translation="ARETPEATPASPDGDPNTIGLVGCVPPSLPVVFTSLAGLSPDAR
                     LYLMQSDGRGRERIGPAGNFQQPVWSPDGKAIAVRFRNPTNGVADWHSEIRLLSPDGS
                     QGVTLFTDESDPEAVTLAQRRDGPSWSPDGQWIGFASPKGGQSWAAWRVSRSGGEPQL
                     LLPGVELSHYDVSWSPRSLDTVAMIAESDGIQDLYLVRNLKSPSLENLTQGRVTEPRL
                     PRWSHDGLTLAIVGGDPQRAPSRDVFAVEVEGHTLMRVTQTADYEVDATWSPSGTELL
                     SAAQPPGVSSPPRQLDLWMISAEQARPARRVTENQGSNWGADWFRGCESSEREQ"
BASE COUNT         1241 a         2139 c         2023 g         1221 t
ORIGIN      
        1 gatgatcaca tcgatcggtt cgctgtcgag cgcgtgtagg ccctctttcg ccgtgactac
       61 ggcggtcacc cgagcgccgg cgtgttctag cacgcgttgc atcgccgcct gagtgctcga
      121 atcatcttcc acgagcagga cgcgcaccga ctcgagggga gcggcggcgc caaagccgcg
      181 accatggagt tcggattcgg cctcgttcgc caccgacgaa tcatcttgaa ttagcggcag
      241 ctcgacgcgg aaggttgcac ccatgccgcg cccatcactc tgggcggtga ttcttcctcc
      301 gtgcaactcc accagttgtt tggcgagcgc gaggccaatc ccgagtccca ggtgtgcgcg
      361 cgttgggccc atatccgcct gccagaactt gtcgaatact cgtggcacgc tctcgggcgg
      421 gatcccttca ccggtgtcct tcacctcgaa ctcgagcacg gcgtcagtgg cggagattcg
      481 caggctgacg ctgcctcccc ggggcgtgaa cttcagcgca ttcgtgagca gattccagag
      541 cacctgcttg atgcgcgcgc cgtcgagtac ggcaagcgtc acctcatcac tgattcgaat
      601 agtgagcgcg atcgatttct ccaaggcgat aggttcgatc atctccatcg ctgcttcacc
      661 caagcggcgc acgtcgtgcg gagctcggct cagcgccagc ttgcccgagg tgagccgcga
      721 caaatcgagc aaatcctcga tcagtcgcga ctggctgtct gcgctctggg taatcgactc
      781 gagcgcgcgc ggcatgtcct tgctcggcac ggctccggac ctcaaagctc ccgcccatag
      841 ctggattgcc gcgagcggag tgcgcaactc atggctcaga gttgcaagga actcgtcctt
      901 cgagcgattg gcagcctcca cctgcgcgcg cgccgcggtc gcttgctcga gcaggcgccg
      961 cgcgagccgc tcgctctcgc ggtgggcggc ctccgtgcgt ccacgctcga ccgcgatcca
     1021 aaggcgctct gagacttgtt ggacgagatc gacgtcgtgc tccgtccagt cacgcgggcg
     1081 gggatcgagc acggtcaaag aggccagtaa cctcccgctt cggacgatag gaacggtcac
     1141 atttgccgca cgaaactgaa caccttgctt atcgagggga atcgtcgaaa aactccgact
     1201 ggactctacg aggggcatgc cctcgcgaag ccggctcgag ggaagaggga agtcgacgag
     1261 cgcgtactgt cctttcaaac tcggcacgtc gccgaccagc atctcggtca gcaccagggc
     1321 gaagccagct tcatcgtcgt agatgacgct aaagcagcta gccccgaaat agtcccgcaa
     1381 gagctcggtt gctatggcgt ggatatctag cggaccggcg gcggcgcgca cccgatccga
     1441 gaaggccagc aggcaagtct tccgagcgtc gtcgtcgagc aaggtctgct cggctttctt
     1501 tcgagtcgtg atgtcatcgt agatcatggc aaagcggtcg tcagcgcggg gatgtacccg
     1561 cacgtcgaac cacctatcca gggcttcgat gtacttctcg aagcgagtgg gacgggaggt
     1621 ctccacggca tggctatatc gcagaagagt ttccttcatg ggatcgcgca gcagctcgct
     1681 ccgaagtctt ccgacaaaac ggccgcgtgg aagtcccaac aggttctcga aagctcgatt
     1741 gagctccagg tagcggatat ctttcgtttg gcccgcttcg tctcggacga tttcgcacag
     1801 cgcgtagcct tcctccatgg cctcgaacag caggcggtat tcttcatcgc gctgtcgtag
     1861 agccgcttcg gcgcgggcgc gttgaaccgc ggcccacgtg cgttcggcgg tctcctggat
     1921 gagcgacacc tcttcttgcg accaccggcg agcccgcgcg gcgtgcaccg agatgttggc
     1981 caccaaacga ccctgtttga ccaggggaac gcccaccaaa gccgcgattc cgagcccgga
     2041 gtaagcactg cgctctcgcg gcgtgagctc ttgcgagacg gcaacttcgt cgatcacgat
     2101 cgtttgaccg gcgaacagcg ccgtcacgag gtgctctccg aagtcgcgca ttcgaaagcg
     2161 ccccaaaagg ttcggcgccc ccgcctcggc gtagccgcga tcgaagcgga tgtgctcgtc
     2221 atcggcttcg acttcgccat acatcactcg agtcacattc agatgagagc caagcagctg
     2281 cgccgcggtt ccctggattt cgatgggatc tgcgagcggc cgtagcgagt cggtcatgca
     2341 cgcgagcagc tgttgtgagg tcgcgggctc gtcggttctg cgctcctgcc agaccacgaa
     2401 ggtgccgcgg aggccaccct ccaagtcacg aatcggaata cattcgagtt cgaacgacga
     2461 ccgctccacg ctagcccgtg taacgtcccg aaatgaccag cccagctcgg cccagccgtc
     2521 ggtcgagttg acagtcgtga gcacttgccc cagcagcgcc cggtacgtcc ccgtcgtttc
     2581 cgcgtaggat gagcgtacgg ggcgccccag cagctcggtg tggctcccgc ctttggagcg
     2641 ttctccagga cccaggaacg caaggctcgc ctgattacag aacacgattg ccgagcgtcc
     2701 ccaaaggacc agaaccggct tcgagaagcc cattgcgagc tgaaggttcg atcgaagtgc
     2761 agctgaccag tgccgcaccg gcccgagttc gcttcggcac cactcgattt ggcgagctat
     2821 cccggcgatc tcgccttcaa tcccgaaagt tgcgcagata gccgcgagct ccgggtcatt
     2881 ggaggagcca aacgacatgg actgaagcag gctagtccat ttctccggag ctgacgacca
     2941 ctgttagacc ttccgcgggt cctcggttcg gtgcggcgcg gccgcgagca gtttcatggg
     3001 tgcagtgcag cacacacagc cggaggagcc ttgctgcctt gggtcaggcg tttcaaggag
     3061 cggcgccggt ttcaatcgtg gcgttcgctc cggacgaagc gatgctcacg agcgtgagtc
     3121 gcagatcggg gccactcgcg ttgtgatcac tgggcgcgga ccgcgcgagc ttcatggatg
     3181 agcctggtcc gagcacttgg agtgatccgt tcaccgtggt tccgctcggg cccagcaagc
     3241 tcgccgcgcc cacgacttca cctggatcca cctcgaagta gtcggcatcg aacgagccgg
     3301 gccgcgaata catggaggag atggccccaa tgccgagtac gtcgaaaact ctcccggtcg
     3361 aagcagtcga gatactgctc tcaccacgcg tgattcgcgt ggagacgcgc cattgggtgg
     3421 aaccgtcggt gctcgggaga tccatgacca gcgtccactc cacctctagc gggcgaaacc
     3481 aatcgagatc gtaccaagct ccatatgcct gaagccgcgg ccgcccagac tcgtcttctc
     3541 cgagcacgag gcggtcaatc actggatgga gctctgggcg cgggggttcg gtcgcgggct
     3601 gctcgaccgg caggaacggg ggcaacacga agctgcttcc aaagcagcgg tccggaacgc
     3661 tcagcgtctt catgcgtagg aagccgttcc agtaaagaac catcgcttgc ggccaaccgt
     3721 gcccgttgtc gtggtgaaga atgactgccg tcgcgcgccg ccactcccgg ctcaaggcag
     3781 cgttgggtgg gagctcgacc tccatcgtcc cggcgccctc ctgattgagc aacttccaaa
     3841 catacgggcg gcccgaactc gcggggcgga tgtccgggga catacactgt ccagtgaaac
     3901 actcggcgat gggtgggcta tatccgggta cgggctcgga tagggggtgg cactcgcgga
     3961 aattcgcagc gcaagcgggc gactcttgga ctcgtggatc cgtcgcggcg agcaccaatt
     4021 ctatgttcaa gacgtcctgg ttctcgggaa gagcgttcgc caataccgcg ctcgccaaga
     4081 atgtgtcttc ttcccgacac tcgtggagaa ttcgacgtgg gaacgccgcg acgcagcttg
     4141 tctcgcccgg tatcaagctg caagcgggag cctctgcggt cgcgccccct cgtgcttcgc
     4201 gcgtagaagc ggcgcttgca tcctcttcaa cccagccaat ggcggagccg ctcgtcgaat
     4261 ctggctcggc tcggcaagcc aacgccaccg tcaatgaaac cataacgccc gacagcccat
     4321 atcgatacat gtttgcgctc ctgagtggct caggctccgt aggccgcgag cggctcacgc
     4381 tccctcggcc gcagctcgct agatagcatg tttgcgctta cggtcgaaag gcctgcaggc
     4441 gcctcgctca tgcgtgaaag cacgcttcgc gcgcgctcgt tcttagagct tcactctaga
     4501 tatcggggga ttgatttgct ggccgcgagt tcgttggtgc gatttcaccc agccatgcgg
     4561 agatatcgcg cgcgcacgcg tttttacctc acgttttacc tcacggtctg cgggtcccgc
     4621 gcgcagcgcg cgccgagccc ggattggcgc atgccgggag cctgttgggc gcgagaagcg
     4681 ctccgataca gtgagtccat gccggcgcag caatcgctgt ctcgagcgcc tcgtactact
     4741 ctacccagtt gcggactcag gttggcacaa tctctacact gcgcgccgac ccgctcatag
     4801 aacccggcgt cgtaccaatc caatgtccat tcttctgcgc tacccgccag atcctcatgt
     4861 ccaaaggcgc cgcgcgtttc cggagaactc ccgaccgggc gtggcccgct ctcgcgtaaa
     4921 ctcgtgatga gcgatggatc cgcgccccac gggtaacttc gattcaactc gccgccggcc
     4981 gccgcaaact cccattctgc ttcggtgggc agacgccccc cgtcccacac gcagaacgct
     5041 agtgcggtga accagtcgag gccagtcatc ggcaggtttg gatcgctggg caaatctgcg
     5101 gccaccgctt ccaggcccgt gctcatggag gggagcaacg cggaaacatc tctatcgaga
     5161 cgagcatccc actcggcatg ccacccgctg ttcgcgatct gtgggtgcgc gccgtcaccg
     5221 tcagccgggg gcgggggagc gtgccgcgcg aacgcagcat aacgaccgac cgtgatttcg
     5281 aagcggtcga gatagaagcc gctcacggtg accgggtgat cggcgctggc ccgctgcaac
     5341 acctgaggaa acggcacctc cttgggatcg aaccccatcg ggaaggtgcc acccgataac
     5401 caaatgctct cgcaacagct ggacccgttc gagctcgccg cgccgcaacg gaggtcggac
     5461 tcacaggaga ggggcatccg cgccggcgga atgacgtcag cgtctggctt catacccgaa
     5521 caaccgaagg acagaagaca gagcccaagc gcgaggcttc gaaaagcact ctcgtgtctg
     5581 ttgcgccggt acaccgtcat ttcgataaag cccacgtggc ccactcttct ctattgttca
     5641 cgctcggacg actcgcagcc tctgaaccag tccgctcccc aattcgaccc ctgattctcg
     5701 gtaacgcgac gagcggggcg agcctgctcc gctgagatca tccagagatc gagttgacgg
     5761 ggaggactgc tcacgccggg cggctgcgcg gcactcagca gctcggttcc gctgggcgac
     5821 caggtcgcgt ccacttcgta atcagcggtc tgcgtgacac gcattagagt gtggccttcg
     5881 acttcgaccg cgaaaacatc gcgggatggt gctcgttgag ggtcgcctcc aacgatggct
     5941 agcgtgagcc cgtcatgaga ccaacgcggg agccgcggct ccgtgactcg gccctgcgtg
     6001 agattttcga gggacgggga tttcaaattg cggaccaaat acaaatcttg gataccgtct
     6061 gactccgcga tcatggccac tgtgtccaaa gaccgaggcg accagctcac atcgtagtga
     6121 gatagctcta cgcccggtaa tagaagctgc ggttcgccgc ccgatcgaga aacccgccaa
     6181 gcggcccagc tctgaccgcc cttgggtgaa gcaaacccaa tccactgacc atcgggtgac
     6241 caactcgggc catcgcgtcg ctgcgcaagg gtcaccgctt cgggatcgct ctcgtccgtg
     6301 aacagggtca cgccctggga gccgtccgga gagagcaacc gaatctcgct atgccaatct
     6361 gcgactccgt tggttggatt gcgaaagcgc accgcgatcg ccttcccatc gggggaccac
     6421 acgggttgtt gaaaattacc cgccggcccg atgcgctctc gtccgcggcc gtctgattgc
     6481 atcaagtaaa gccgtgcgtc cggcgatagg ccggctaaag acgtgaagac gaccggcaac
     6541 gatggcggca cgcagcccac cagtccgatt gtgttggggt ctccatccgg gctcgcgggg
     6601 gtggcctccg gggtctctcg cgcc
//
LOCUS       DASTKU010000578         9517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_781271, whole genome shotgun sequence.
ACCESSION   DASTKU010000578 DASTKU010000000
VERSION     DASTKU010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9517
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781271"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFQ61_20120"
     CDS             <1..756
                     /locus_tag="VFQ61_20120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956818.1"
                     /translation="LAKFSFFVTSLGAMRDLAKEFGAGDQGFGGDLRYGEATGLAGAD
                     KICRTIAERAMPGAGKKTWRAFLSATKGGENDGPIHAKDRVGKGPWYDAVGRLVATNL
                     EDLLQDRPRGADPAIINDLPNEFGIPNHKDGAPGCTGMACPDNHQVLTGTNEKGMLYS
                     FSTSGQMGGTQYTSWDATCHDWTTREQVGRPWCGHSWPRQGSGTNWMSSYYDAGCAPC
                     IAITERGGVLTSEACVGSAGGYGGFYCLAINSP"
     gene            836..1957
                     /locus_tag="VFQ61_20125"
     CDS             836..1957
                     /locus_tag="VFQ61_20125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956819.1"
                     /translation="MRTHWLSSNSLGALSPGHTLALLAVLVFANGCTSKNDNASPAAG
                     ASSGGASMAGSTAMGGASGGTTGSGGTTAGTTTAGTNPAGGSTSTAGNQGSGGSSVSG
                     PLYGGFVVGLEPAADGAPAYTSVLGRFYDGPTPATIPLKEARAQGNCKLLVPFVPFCE
                     PMCDTSSAVCTADNVCTPKPKPIAVGEVQLSGLGSAPLTLKPGGASAYQPSSSLPNPP
                     CQPGAEVNLAWNGPAMRTTCIAALEVTTPSPIPVKTGSSVKLAWTPPATNANTRVRIA
                     MDLAHHGGKKGEIQCEVDDTGSFEIPEPLITDLVQLGLAGFPTISLDRVSVGKVEALP
                     NVTLVMSSVVTLPVDTGVVSCVSDTECNGKTCGVDRACH"
     gene            complement(2072..4426)
                     /gene="mdoH"
                     /locus_tag="VFQ61_20130"
     CDS             complement(2072..4426)
                     /gene="mdoH"
                     /locus_tag="VFQ61_20130"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HET9956820.1"
                     /translation="MAAATLQADPELHARAAICHVQREFESWKTAVFEGFQSPPNPLW
                     LRAFLGSYPKLFLGDVAQARTAAARFGDASEGRLPRRAQFHDQKLEAARLPHWLSGLL
                     PALLLTLAAVSGLASALFTDGLSWLELLWLGCFAFLFGLSAVGLSTAVRGFFPTTRRV
                     ATQSLARAEIDRRVRGQVNVETQSRPGAAAAPLPRTALVMPVYHEDAGQVFAALAAMR
                     ENLLQIPGHEAFEIFVLSDSRDPLCAAEEERAFRRVAKTAAEQIPFFYRRRARNERQK
                     AGNLAEFFERWGTRYRYVIVLDADSLVQPDTLIELVRRMEAAPRLALLQAPITLYGGN
                     TLFARMLQFSNSLCGPLFTRGLARWSEGQANYYGHNAIVRVAAFLECCALPKLEGEAP
                     LGGHILSHDFVEAALLCRAGWQVRIADDLTGSFEGLPPTLLEYVARDRRWCQGNLQHL
                     QVASSRGLKTMSRIHLGLGAAAYLAGPLWLGFLILTFFCYASGEWLSDTAQAGWFAAA
                     STGVLLLGPRWLGWLEVGLDSKRRRSQGGWLRLSASVLAEQLLSALLAPLMMLHHTRI
                     VLSILLGRAVGWRAQNRTATHPLGRIIAIEAPATLLGAAAFLGLRNWAPGLVSWLAPI
                     WLPWCAAIFVHGGLSSERLGQWSRRLGLFLIPSETEPSELMQRARELCAFTASDDAGR
                     FRDLVLDPVLLAAHVAKLERDALGSPARHSGSGARAESLQRLRERALRAGPAALSVSE
                     RERLSADPESMSWLHRTAWQHWPVESWEISRERPQVPEEPGASL"
     gene            complement(4663..6444)
                     /locus_tag="VFQ61_20135"
     CDS             complement(4663..6444)
                     /locus_tag="VFQ61_20135"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HET9956821.1"
                     /translation="MRFESTCTRSTPSGAQSTFPKKASTAQTKCGPRNQRMWPARAIS
                     SGILVLGCGVAQQPGSSPMIPERHRQAVTSSSSVAVHGEKPAGAAHPGPEYAGSSSVS
                     LADFVQQLERELPQSSWRELVERAHELSKQSARPPRKIELPPSLKGLRYDDFRGIRFR
                     PERSLWRGGAGQFEAQFFHPGFYFQEVVDVFEVKRTATGADSRRVEFSTDQFSYEGIP
                     NPPRGAALGFTGFRLHAPLNQTQYRDELVAFLGASYFRPLGKDTVYGLSARGLAMDFG
                     APTSEEFPRFTEFYLLEPAANQRQVWVLALLESRRATGAYAFRFEPGTMTHVDITAQL
                     FLRESVSVLGAAPLTSMYLFGEDAPGRFGDFRPEVHDSDGLAIWGRDGEWLFRPLRNP
                     PRTTTSSLRADQPRGFGLVQRDRSFDSYQDLEARYQDRPSVWVEPISGFEGGAVRLLE
                     ISTELETDDNIVAAFVPDRTSLEPLLRYRLHVGRQSPFEAAGGRVVATRLAKTKNGQR
                     FLVDFRGAALEARDGVPPPEVRCVASATGGRIVEQHVEKNPYLDGYRASFEVTPEPGA
                     KDVELRAFLRAKDVVTETWSYLWQPTR"
     gene            6538..8673
                     /locus_tag="VFQ61_20140"
     CDS             6538..8673
                     /locus_tag="VFQ61_20140"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9956822.1"
                     /translation="MNSRSLRSLDIAARSLLESQAASGTLASVIAEGALAHHFSGLFQ
                     YAASRIGTGPALAALERLEQEFENEPALDGEAGSFGVGARLYRRLRITLRSEPTVALA
                     PDAPEWWTPPERSLAACLIELRCKLDADQAEVLELCFARGLPVPDAAYVLELAPDATE
                     RCLTAALAVVDQTLRSEVRSGRPRELSLLQAFALDPRYVQAPRRVPRKAMLGVGDVIQ
                     QRYQIEEALGAGAFADVYRARDLEVTDHVVALKILRRPAADAHATRAALRELQLIASV
                     FHPSIVQLKDHGWHLGHLWFVMPLYRGETLRERLRRGPLKRAEARQVFEPLAEALATM
                     HRAGVRHQDIKPENVFLANIDPESDSHPSSSGARILPVLLDLGVAAKDAELVLAGTPA
                     FFAPEVAARFAGVPDPPPVGPKSDVFSLALTLREALDPSPTASLATGAVDAFVTFRAG
                     HGASPPRRAELSDLRGCFETWLHLSPDVRPSAEEFRNQLGALTRPAERRARRLELMRW
                     AVPIAFTVLVLFVAVVFVLSREAKLQRIDADTERARAERARDRAASMHASLEEEEARR
                     RKLESDISRLEGAYQNSRMTREQLAARLANAEGETSALLDRQQLQQSRLRQQSEEVRQ
                     LRELGARLVGETESLRRRRDELASELDRTKAALDLERARHAETAARTETVTLQLRAAE
                     VVLEEARARNRELETHLNQAPSKPMSPKD"
     gene            8879..>9517
                     /locus_tag="VFQ61_20145"
     CDS             8879..>9517
                     /locus_tag="VFQ61_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956823.1"
                     /translation="MKPVSSRAEGVSVLRSTGACLLLAGLLSGTACQPQAPATTPPSA
                     TDANKPPAAAPTALGPASNATIPNVPAQQIAISAAVRAAVDAGDRDAEDRALDSGRHP
                     VEVLSFFGIGPGQRVAELFAGGGYTAELLARVVGAQGRVFAQNSRFILERFAQGPWTE
                     RLKKPVMRNVVRVDRELDSPLPPEARDLDAVLFILSYHDSVWMNTDRAAMNRN"
BASE COUNT         1837 a         3053 c         3010 g         1617 t
ORIGIN      
        1 ctggcgaagt tcagcttctt cgtgaccagc ctcggcgcga tgcgcgacct agccaaagaa
       61 ttcggagccg gggatcaggg gttcggtgga gacctgcgct acggcgaggc cacgggcttg
      121 gccggagcag acaagatttg ccgtacgatc gcggagcgcg cgatgcccgg cgccggcaaa
      181 aagacatggc gggccttctt gagcgccacc aaaggtggcg agaatgacgg gcccattcac
      241 gccaaggacc gtgttggcaa ggggccttgg tatgacgcgg tggggcggct cgtcgctacc
      301 aacctggaag acttgctgca ggatcgcccc cgcggagccg atcccgcgat tatcaacgac
      361 ctgccgaacg agtttggaat tccaaatcac aaggacggtg ccccaggttg tacaggcatg
      421 gcctgcccag acaatcacca ggtgctgacc gggacgaacg agaagggtat gctgtattcc
      481 ttctcgactt ccgggcagat gggcggcacc caatacacat cgtgggacgc aacctgtcat
      541 gactggacca cacgagaaca agtcggaagg ccctggtgcg ggcactcctg gccacgccaa
      601 ggtagcggca ccaattggat gtcctcgtac tacgatgccg gctgcgcgcc ctgtatcgcc
      661 atcaccgagc gtggcggcgt gctgaccagc gaagcttgcg tcggctccgc gggtggctac
      721 ggcggcttct actgcctggc tatcaactcc ccctgaacga tctcgacgag ctcactttcc
      781 tggcccgtcc gatagtccgt cttatctaag ccctgcacac tcgcccagaa acatcatgcg
      841 cactcattgg ctttcatcga actcgctcgg agccctttca cctggtcaca cgctcgcgtt
      901 actggccgtg ctggtatttg cgaacggctg caccagcaag aatgacaacg cgagcccggc
      961 agcgggcgcg agttccggtg gagcctcgat ggccggcagc acagctatgg gcggagcgag
     1021 cggcggcacg acagggagcg gtggaaccac ggccggaaca accacggccg gaacgaaccc
     1081 cgctggcggg agcacgagca cggcaggcaa ccagggatcc ggtggttcat ccgtctcggg
     1141 cccgctttat ggtggcttcg tggtcggttt ggagccggcg gcagacggcg cgcccgccta
     1201 tacgagcgtg ctcggacgct tctatgatgg ccctactccg gccaccatcc cgttgaagga
     1261 ggcgcgtgcg caggggaact gcaagctgct ggttcccttc gttccgttct gcgagccgat
     1321 gtgtgacacc agcagcgccg tgtgcactgc agacaacgtc tgtaccccaa agcccaaacc
     1381 cattgccgta ggggaagtcc agttgagtgg cttgggaagc gcgcctctga ctctcaagcc
     1441 cggtggagca tcggcgtacc agccgtcgtc ttccttgccc aacccgccct gccagcccgg
     1501 cgccgaggtc aatctggcgt ggaacggccc ggcgatgcgc accacgtgca tcgcggcgct
     1561 ggaggtcact acgccgagcc ccataccggt gaagacgggc tcatccgtaa aacttgcttg
     1621 gactcccccc gctaccaacg ccaatacgcg cgtgagaatc gcgatggatt tggcacatca
     1681 cggcggcaag aagggcgaaa ttcaatgcga ggtcgacgac accggctcgt tcgagatccc
     1741 cgaaccgctg atcaccgact tggtgcagct tggcttagcc ggcttcccca ccatctcgct
     1801 cgaccgagtt tcggtcggaa aggtggaagc tctgccgaat gtcacattgg tcatgtcgtc
     1861 ggtagtcacc ctgcctgtgg ataccggcgt tgtttcctgc gtgtcggata cggagtgcaa
     1921 cggaaagact tgtggagtcg atcgagcctg ccactaaagg tcgcgaatgg agaatcggag
     1981 gccctcaggg cggaagcggg cccccgattc gcagacgagt agcctacgaa tagcccacga
     2041 gtagcccact ttggaccctc tggcgctgtg gctacaggct agcgccaggt tcctcgggaa
     2101 cttgcgggcg ctctcgcgag atttcccaag actcgacagg ccaatgttgc cacgcggttc
     2161 tatgcagcca cgacatggac tctggatctg cgctcagacg ctcccgctcg ctaacgctca
     2221 aggccgcagg cccggcgcgg agcgcccgct cgcgaagcct ttggagagac tccgcgcgcg
     2281 ccccagagcc cgagtgacga gcgggtgaac cgagcgcatc tcgttccagc ttggcgacgt
     2341 gagcggccag cagcacagga tccaatacta agtcgcggaa acgtccggcg tcatcgctcg
     2401 ccgtaaaagc acacagctcg cgagcacgtt gcatcaattc gctgggctcg gtctcgctcg
     2461 ggatcaagaa caacccgagc cgacggctcc actggcccaa tcgctcgctc gaaaggccgc
     2521 catgtacgaa gatggccgca caccacggaa gccaaatggg ggcaagccac gacacgagcc
     2581 ctggcgccca gttgcgcaaa ccaaggaagg ccgctgcacc cagtagagtt gctggtgctt
     2641 cgatcgcgat gatgcgcccg agtggatgag tagcagtgcg attctgggcg cgccatccga
     2701 cggccctacc caacagaatg gacagcacga tgcgggtgtg gtgcagcatc atcaacggcg
     2761 ccagcagcgc cgagagcagc tgctcggcga gaacactcgc gctcaatcgc agccatccgc
     2821 cttgcgatcg acgacgtttg gagtccaagc ctacttcgag ccaccctagc cagcgcggac
     2881 caagcagcag aacaccagta ctcgccgcgg cgaaccaacc tgcttgcgcc gtgtcactga
     2941 gccattcgcc cgatgcatag cagaagaacg tcagaatcag gaatccgagc caaagcggcc
     3001 ccgccaggta ggccgcggcg cccagcccca agtggattcg actcatggtc ttcaagccgc
     3061 gcgagctcgc cacttgcagg tgctgcaagt tgccctgaca ccaacggcga tcccgagcca
     3121 cgtattcaag tagtgtgggg ggcaggcctt cgaagctgcc ggtcaagtca tcggcaatcc
     3181 gcacttgcca cccggcacgg cacagcagtg cggcctcgac gaaatcgtga cttaggatgt
     3241 ggcctccaag cggcgcctcc ccttcgagct tgggtaatgc acaacactcc agaaaagcgg
     3301 ccactctcac gatcgcgttg tgaccgtagt agttggcttg accttcggac cagcgcgcta
     3361 acccacgagt aaacagcggc ccacagaggc tgttggagaa ctgcagcatc cgggcgaata
     3421 aagtattgcc gccgtatagc gtgatcggag cctgcaagag tgccaaacga ggcgcggcct
     3481 ccatgcgccg caccagttcg atcaaagtat cgggttgaac cagactgtcc gcgtccaaga
     3541 caatcacgta ccgatagcgc gtcccccaac gttcgaagaa ctcggcgaga ttgccggctt
     3601 tttgccgttc gttacgggca cgtcgtcgat agaagaacgg gatctgctcg gcggcggtct
     3661 tggcgacgcg tcgaaatgcc cgctcctctt cggcggcgca cagcgggtcg cgcgaatcac
     3721 tgagcacgaa gatttcgaat gcctcgtgcc ccggaatctg cagcaggttc tcgcgcatag
     3781 cggcgagcgc ggcgaacact tgtcccgcat cctcgtggta caccggcatc acgagcgcgg
     3841 tccgaggtag cggcgcggcg gcggcgcccg ggcgtgattg cgtctcgacg ttcacttgcc
     3901 ctcgaacgcg acgatcaatc tccgcgcgag ctagcgactg cgtcgcgacg cgcctcgtgg
     3961 ttggaaagaa gcccctcacg gcggtgctta ggccaacggc cgagagcccg aacaggaacg
     4021 cgaagcaacc taaccaaagc agctccaacc aactcagacc gtccgtgaat agcgcgctgg
     4081 ctagaccaga cacggccgct agcgtcagca ggagcgccgg cagcaaacca gagagccaat
     4141 gaggtaggcg agccgcctcg agcttttgat cgtgaaactg cgcccgtcgc ggcagcctgc
     4201 cctcactcgc gtcgccgaac cgagcggctg ccgttcgcgc ttgggccacg tcgccgagga
     4261 atagcttcgg atagctgcca aggaaggcgc gcagccaaag tgggtttggt ggcgactgaa
     4321 agccttcgaa tacggcggtt ttccaggact cgaattcgcg ctgaacgtgg catatggccg
     4381 cccgcgcgtg cagctcggga tctgcttgca gggtggccgc cgccatccgc ccgctcaact
     4441 ctgcaatgcg gcttccttcg accaacccaa gagtgcgcag gtactccgcg acacggcgtt
     4501 cggcccgagg tgcggaaggc tcgatatcac gtaccccact aagctggttc gagttgccag
     4561 ctttcgggtc acgcgatttc gaactagcgg ttcgcgagac ctcggcgttg ctccaggttg
     4621 acccgattgg gcctcgagcg gcctgctcgg attcgggacg gtttagcggg tgggctgcca
     4681 aaggtaactc caggtttcgg tcaccacgtc cttggcgcgc agaaaagcac gtaactctac
     4741 atccttggcc ccgggctccg gcgtcacctc gaaggatgcc cgatagccgt cgagataggg
     4801 gttcttctcg acgtgctgtt cgacgatgcg gccccccgtc gccgaagcca cgcagcgcac
     4861 ctctggggga ggcacaccgt cgcgcgcttc tagcgcggca ccccggaaat ccacgaggaa
     4921 tctttgcccg ttcttggtct tcgccaagcg ggtcgccacc actctaccgc cggcggcctc
     4981 gaacggcgat tgccgcccca catggaggcg gtaacgcagc agcggctcga gtgacgttcg
     5041 atccggtacg aatgccgcga cgatgttgtc atcggtttcg agctcggtgc tgatctcgag
     5101 caaacgaaca gcgccgccct cgaagcccga aatcggctcg acccagacac tcgggcgatc
     5161 ttgataccgc gcctcgaggt cttgataact gtcgaagctg cgatctcgtt gaacgagccc
     5221 aaagccacgc ggctggtccg cacgcaggct cgacgtagtg gtccgcggcg ggttgcgcag
     5281 cggccggaac agccactcac cgtcccgccc ccaaatcgcg agcccatcgg agtcgtggac
     5341 ttcagggcga aaatcgccga aacggccggg cgcgtcttct ccgaagagat acatgctcgt
     5401 gagaggggcg gcgcccagca cggatacaga ctcgcgcagg aagagctggg ccgtgatgtc
     5461 cacgtgggtc atcgtccctg gttcgaagcg aaacgcgtac gccccagttg cacgccggct
     5521 ctctagcagt gccaataccc agacctgccg ctggttggcc gcaggctcga gcaagtaaaa
     5581 ctcggtaaac cgtggaaatt cctcggacgt tggcgcgcca aaatccatgg cgaggccacg
     5641 cgcggaaagg ccatatacgg tatctttccc gagcggcctg aagtagctcg cacccaagaa
     5701 agccacgagc tcatcgcgat attgagtttg attgagcggc gcgtgcagcc gaaaccccgt
     5761 gaagccaagt gcagctccgc gtggggggtt gggtatcccc tcataactga attggtccgt
     5821 cgaaaactct acacgacgcg agtccgcacc cgtcgccgta cgtttgactt cgaagacgtc
     5881 gaccacctct tggaagtaaa acccggggtg gaagaattgc gcctcgaact gacccgcgcc
     5941 gccacgccat aggctacgct cgggccggaa gcgaatgcct cgaaagtcat cgtagcgtag
     6001 gcctttgagt gacggaggca gctcgatctt gcggggcgga cgggcagatt gtttgctcaa
     6061 ctcatgagct cgttcgacca gctcgcgcca actactctga ggcaattcgc gctcgagttg
     6121 ctgcacgaaa tcagcgagcg agaccgagct cgacccagcg tattcgggcc ccgggtgcgc
     6181 cgccccggct ggcttttcgc catggaccgc gacgctcgag ctggacgtca ccgcttgtcg
     6241 atgccgttcc ggaatcatag gggacgagcc gggctgttgc gcaacgccac aaccgagcac
     6301 caatatgcca ctcgaaatgg cccgggccgg ccacatgcgt tgattgcggg gcccgcactt
     6361 ggtctgggcc gtggaggcct tcttcggaaa ggtcgattga gcccctgacg gcgtcgatcg
     6421 cgtgcaagtc gattcgaaac gcattcggtg ttcggctatg taacacacga gtgccggtac
     6481 acaatggaac tgctcggaag attctccacc gcggtcggta gtttgggacg agacagcgtg
     6541 aactcgcgca gcctgcgttc gctggatatc gccgctcgat cactgctgga gagccaagcc
     6601 gcgagtggca ctctcgcgag tgtaatcgcc gagggtgcgc tcgcgcatca cttctcgggc
     6661 ttgtttcaat atgcggccag ccgcatcgga actggccccg cgctcgctgc cctggagcgc
     6721 ttggaacagg aattcgaaaa cgagccagcg ctagacggcg aagcgggaag cttcggggtg
     6781 ggcgcgcgcc tataccggcg tctgagaatt acactccgga gtgagccgac cgtggccctc
     6841 gcgccggacg cccccgaatg gtggacgcca ccagaacgca gcctcgccgc gtgtttgatc
     6901 gaattgcgat gcaagctcga cgccgaccag gccgaggtgc tcgaactctg tttcgcgcga
     6961 gggctgcccg tgcccgacgc cgcgtacgtt ttggagcttg ctccggacgc gactgagcgt
     7021 tgcttgacgg ctgcgctggc ggtggtcgat cagacgctgc gatcggaggt gagaagcggc
     7081 cgtccgcgag aactgtcgtt gctccaagcg ttcgcgctgg acccgcgtta cgtgcaggcc
     7141 ccccgacgcg tgccgcgcaa agccatgctt ggcgtaggcg acgtaatcca gcagcgatat
     7201 cagatcgaag aggcgctcgg tgcaggcgcg ttcgcagacg tataccgcgc gcgcgacctc
     7261 gaggttaccg accacgtcgt ggcgctcaag attttgcgtc gccccgccgc ggacgcgcac
     7321 gccacgcgag ccgcgttgcg cgagctccag ttgattgcat cggtcttcca tccctcaatc
     7381 gtgcaactca aagatcacgg ctggcacctc ggacatctct ggttcgtcat gccgctctat
     7441 cgaggcgaga cgctccgcga gcggttacga cgcgggccgc tgaagcgcgc cgaggcacgc
     7501 caagtgttcg agccactggc ggaggctctc gccaccatgc atcgagcggg ggtgcggcac
     7561 caagacatca agcccgagaa cgtgtttttg gccaatatcg atccggaaag tgactcgcat
     7621 ccgagtagca gcggcgcgcg tatactcccc gtgctgttgg acctgggggt agcggccaaa
     7681 gacgccgagc tggtgttggc gggcacacca gccttctttg cgccggaggt cgccgcgcgc
     7741 tttgcgggag ttcccgatcc gcctcccgtc ggccccaaat ccgacgtatt ttcgctagcg
     7801 ctaacgctgc gagaagcgct cgatccgtca ccgaccgcct cgctggcgac gggagccgtg
     7861 gacgccttcg tcaccttccg tgccggtcac ggcgcgagcc ctcctcgccg ggcggagctc
     7921 tccgacttgc gaggctgttt tgaaacttgg ttgcatctat ctcccgacgt tcgcccgtca
     7981 gcagaggagt tccggaacca actcggcgcc ctcacgcggc ccgccgaacg aagagcgcgg
     8041 cgtctcgagc tgatgcgttg ggccgttccg atcgcgttca cggtattggt gctgttcgta
     8101 gcggtggtgt tcgtgctctc gcgcgaagcc aagttgcagc gcatcgacgc tgacaccgag
     8161 cgggctcgcg ctgaacgcgc gcgagacagg gccgcgagca tgcacgcgag cctcgaggag
     8221 gaggaggcgc gtcgacgcaa gctcgagagc gacatcagca gactcgaggg ggcgtaccaa
     8281 aacagtcgca tgactcggga gcagctcgcc gcccggctcg cgaatgcgga gggtgaaacg
     8341 agcgcgctcc tggatcgaca gcagctccag cagagccgat tgcgtcaaca gtccgaggaa
     8401 gtgcgtcagc ttcgagagct cggggcacga ctcgtcggcg aaacggaaag tttacgccgt
     8461 cgccgagacg agctcgccag tgaactcgac cggaccaagg cggcgctgga tctcgagcga
     8521 gctcgccacg cggagacggc agcccgaacc gagaccgtca ccctgcagct cagggccgcc
     8581 gaggtggtgc tggaggaagc acgcgcgaga aaccgagagc tcgagacgca cctcaaccag
     8641 gctcccagca agccgatgtc accaaaggac tagcaacgag cgaacgttcc cggcgcggcg
     8701 ccgttacagg gccggagagc tcgcggtgtt ttcgcctcgg ggcgggttcc cggcccggca
     8761 cgccgcacgc gatgcgggct cggccgtccg gcagtgcaag cctcagattt cgggcttagc
     8821 ccgtggacgc agccaacgac gtgagcaagg atgcgcctcg ccacgaggaa agagctcaat
     8881 gaaacctgtc agttcccgcg ccgaaggcgt ctcggtgctt cgatcgaccg gcgcttgcct
     8941 gttgttggct gggctgttgt ccggcaccgc ttgccaacct caagcgccag caacgacccc
     9001 gcctagcgct acggacgcga ataagccccc cgcggccgcc cccaccgcgc ttggacctgc
     9061 gagtaacgct acgatcccga atgtacctgc ccagcagatc gcaatcagcg cggcggtgcg
     9121 agccgccgtg gatgctggtg atcgcgatgc ggaggaccgt gcgctcgact ccggtcgcca
     9181 cccagtggag gtgctctcgt ttttcgggat cgggccgggt cagcgggtcg cggagctgtt
     9241 tgcggggggc gggtacaccg ctgagttgtt agcgcgagtg gtgggcgcac agggtcgagt
     9301 tttcgcgcaa aatagccgat tcatcctcga gcgcttcgcg caggggccct ggacggaacg
     9361 cctgaagaaa ccagtgatgc gcaatgtggt ccgcgtagat cgcgaactcg actcgccatt
     9421 accacccgag gcgcgcgacc tcgacgcggt gctgttcatt ctctcgtacc atgacagcgt
     9481 ctggatgaac acggaccgcg ccgcgatgaa tcggaac
//
LOCUS       DASTKU010000579         7745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_559081, whole genome shotgun sequence.
ACCESSION   DASTKU010000579 DASTKU010000000
VERSION     DASTKU010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559081"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFQ61_20150"
     CDS             <1..200
                     /locus_tag="VFQ61_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956824.1"
                     /translation="EALPNVPTHFVLCTDDRFFPAKFLRRVVADRLRVVPDEIAAGHC
                     VALSRPRELATLLQSYVKRSC"
     gene            complement(260..679)
                     /locus_tag="VFQ61_20155"
     CDS             complement(260..679)
                     /locus_tag="VFQ61_20155"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9956825.1"
                     /translation="MTIPVRPQPLLAVRDVRASARWYASLLGATRTSELMASDHAHLY
                     DRLLVDGVLVLQLHAWNAEDHPNLIDPDGGRNGHGVLVWFELADLDAAVERARAMQAQ
                     VVEDVHVNPAPAHREMWLRDPDGYLVVICSPDGERGA"
     gene            complement(705..1133)
                     /locus_tag="VFQ61_20160"
     CDS             complement(705..1133)
                     /locus_tag="VFQ61_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956826.1"
                     /translation="MKATTIGSCLAFILLAMSCSSVDDGSGAALETSSGASESGASIE
                     SELRVLTCRSSVECPRLTHCSTEDGVCNRPPGCKPGSICPAVCYGTCVPEKIELPPPI
                     GGGVCGDKVCSAGTFCCNASCGTCAPLGGACTQQVCLPKE"
     gene            complement(1476..2000)
                     /locus_tag="VFQ61_20165"
     CDS             complement(1476..2000)
                     /locus_tag="VFQ61_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956827.1"
                     /translation="MKSGNSGVGCAVVAGLVLSMGCGGTDEPPRTLSPADVTGLPAGD
                     ASGTSFTGAYMLTKSSVTECNCRVGPCSKLHGSQGDLFTLRQTGGALNIVLRSRTGEQ
                     TYDGGINRDGSFQVGSATVDAPYTSYSLLSGTVSAGVSMAATSYNTFTGKVDGEDFDC
                     DATAALEMSFLNAL"
     gene            complement(2076..2972)
                     /locus_tag="VFQ61_20170"
     CDS             complement(2076..2972)
                     /locus_tag="VFQ61_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9956828.1"
                     /translation="MGDPLTEMIQLLQPRAVLSKPISGAGKWAVRYSRFDAPSFAAVL
                     EGSCRLAVDGHRPVTLEAGDFVLLPATPGFTMSGFEAAAPVHIDPKSVPLPTSEVRHG
                     RIGGKPNVRLLGGSFTFGSPDRALLVSLLPGLFHIRGVPRLTLLVQLVAEEARAQNAG
                     RELVLSRLVELLLIEALRSQPGEGAPAGLLRGLADARLTPALRKMHDQLGHAWTVPEL
                     AKAAGLSRSSFFERFHVAVGMRPMEYLLTWRMAVAKDLLRRRELDISLIAERVGYASS
                     STFSTAFSRHVGTSPGRYARAI"
     gene            3055..3846
                     /locus_tag="VFQ61_20175"
     CDS             3055..3846
                     /locus_tag="VFQ61_20175"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9956829.1"
                     /translation="MKTILITGCSSGYGLETARHFHAQGWQVIATMRCPRSDILPSSE
                     RLRVLPLDVTRSESIAAALAESGPIDVLVNNAGIGLFGALEVTPMATIREIFETNTFG
                     VMAMTQAVVPRFRARRSGTIVNVTSSATLAPMPLVSVYTASKMAIEGFTASLAFELEA
                     FDVRVKLVEPGYGPSTAFTKNTGSRLEGLIPEAYAPLAQEIFAALGQGTGAVTKESDV
                     ADAIYRTVNDPSNQLRFPAGADAIALAQGGSPKPQAHGGEPSSRV"
     gene            complement(3816..4742)
                     /locus_tag="VFQ61_20180"
     CDS             complement(3816..4742)
                     /locus_tag="VFQ61_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956830.1"
                     /translation="MSRADRRLANVHKVVRGSVAIFLLLSPAGCSFSQLLPPKEPSKR
                     AAKADKQCGNPLYPIADAASVIAGYTWVHWSNQQERKEANQSMDYDQFGQPITRDSHS
                     SDSRYAVARGFGYGAMVAFGLGAIYGTYVEFKCARLRRETEAATKANAPPVRPGFPES
                     VFEFKFGSALPEAQRACAIRAAEWRTESASAYCSHRTKTMARPDFRFEFALGTLSGIT
                     LRYYTSPERLERDYEALNGAMRRTFGDANTEPSALSRACADSVWACLQQGETRPGPEW
                     HWANGSIELMTRAVDGRPVVEARYTREEGSPP"
     gene            4975..5196
                     /locus_tag="VFQ61_20185"
     CDS             4975..5196
                     /locus_tag="VFQ61_20185"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HET9956831.1"
                     /translation="MSAVKSAFFVKNVPGWERALRLVLAAGALVFGVVQGGMLGAVGF
                     GSAAMLAFSAVLGFCPMCALAGRRLASKG"
     gene            5202..5744
                     /locus_tag="VFQ61_20190"
     CDS             5202..5744
                     /locus_tag="VFQ61_20190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9956832.1"
                     /translation="MALTVSPALFDAARAGDAGAIGRLLELSRPELRRYAQRSCIITD
                     VEDAVQEAMWTLARYVGTLQSVRALTTWLFRVTRRECRRLARKTLGLDLWDDSKTEAV
                     MARLPPPTLRLEVAAAIQSLPEHYRDVLLLRDFEELTIAELAERLALSEANVKSRLFR
                     ARELVREYLLSGGPGAPAGS"
     gene            5931..7700
                     /locus_tag="VFQ61_20195"
     CDS             5931..7700
                     /locus_tag="VFQ61_20195"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET9956833.1"
                     /translation="MNRALLHTSGVAFLTFLVTAGLVSGCAGEANSSVQEEPLVIEAY
                     VEGTVTSGVTHTPIAGATVALPGVMGEAGSTTTDSNGFYSLKAPGVGAHTLFVSAKGY
                     ARAKYSFSANTEPGASGQIQVTAVRNSQLYPTSDADGGALSGRVASGGGRDLIAGAKV
                     LVQFLDTSDPIEDASLKVTAQTDSDGKFMLKNLPAGAPQTRVTVLPADVDDDGKPDTA
                     VFSNFVSGGPSNGVLTPGGQNFMDIVVDPYIGDKVLWTNLDDRPSLDPDEEIVVAYAE
                     SMVDTKDATLVTLAEGADRVKVDFEWEDNLKLTVTPLEPLLQGHTYTLSVQTQSTAGV
                     PVALSRSFLVTSAEAPPDAVTGLEIVDADPVPWSTRRFTLAWDAASDVDGNPIDTYRV
                     LARNDARQTTWTILFEGAVSRFSRPQLQVNLPESFDSFPNEDVFSAVGFGTTVEFAVQ
                     AYSGRNDGPISEEPATFQDTHCPSLTVTPPVTTDNSLGTEELVVKFLISSNDNEPLSD
                     DSEPKFTFADGTLSTDPFVISGAPRVRRVRAGQLEYSFAIPKGKTGAGDTLTLDLSKV
                     TDTSGNGPDGSAGCVKTQSLTLN"
BASE COUNT         1473 a         2475 c         2401 g         1396 t
ORIGIN      
        1 tcgaagcgct accaaacgta cccacccact tcgtgctctg cacggacgat cgcttctttc
       61 ccgcgaagtt cctgcgccga gtggtggccg accggctccg cgtggtcccg gacgagatcg
      121 ctgccggcca ctgtgtcgcc ctcagccgcc caagagaact cgcgactctg ctacaaagtt
      181 acgtgaaacg ctcgtgctga attgggcggc tcaattgggc acggctggat tacgcaccgc
      241 tggattaggc acggctggat tacgcaccgc gctcgccgtc tgggctgcag atcacgacga
      301 ggtagccgtc tgggtctcgc agccacatct cgcggtgcgc cggcgccggg ttcacgtgga
      361 cgtcttcgac cacctgggcc tgcatggcgc gagctcgttc gacggccgca tccaagtcgg
      421 ctagctcgaa ccacaccaat acaccgtgac cattccgtcc gccgtcagga tcgatcagat
      481 tgggatggtc ctccgcgttc caagcatgga gctgaagcac gagcacaccg tccacgagta
      541 ggcgatcgta aagatgggcg tgatcggacg ccatcagttc actcgtgcgc gtggcgccca
      601 gcaggctcgc ataccagcgg gcgctcgcgc gcacgtctcg aactgcgagc aggggttgag
      661 gacggacggg aatggtcatg gggaagttac ctcgtactct acttctactc cttgggcagg
      721 cagacttgct gggtgcaggc accaccgagt ggcgcacagg tgccgcagct cgcgttgcag
      781 cagaacgtgc ccgctgagca gactttgtcg ccgcacactc cgccgccgat gggcggaggc
      841 agctctatct tttcggggac gcaagtgcca tagcagaccg cgggacaaat cgatccgggc
      901 ttacagccgg gggggcggtt gcatacccca tcttcggtgg agcagtgggt cagccgcggg
      961 cactcgacgc tggacctgca cgtcagcacg cgtagttccg actcgatgga agcaccactc
     1021 tcgcttgcac cactgctcgt ttcgagtgct gcgccgctcc cgtcgtccac cgacgaacag
     1081 ctcatcgcca atagaatgaa ggccagacaa ctgccaatgg tcgttgcttt catgactgct
     1141 cctgctcctt gtacgcgatc gtatcgcacg cgatcgcatc gatcgtgccg actatatcga
     1201 ctcgcaatct cgttgattcg cgatctgaat tcaaaggttc gaactgacga ctcgacgccc
     1261 ccccgcgcgc catgaatcat gcacagttcc ggcctttcgt tcaatgggtc tcgccatggt
     1321 cggacataat cgcgtcacga tgccgcccgc gacgaagagc gcagaaaaat gcgaccagag
     1381 acgcctgcac ctcgagttcg acttcacgtg agcccgggcg tcacagcacg agcgcattca
     1441 cccgtggatt agccggatgc gacgatagaa ggctttcaaa gcgcgttcaa gaaggacatc
     1501 tccagcgcag cagtcgcgtc gcagtcgaag tcctcaccat cgaccttacc cgtgaacgtg
     1561 ttgtatgacg ttgcggccat ggagacgccc gcgctcacgg ttcccgagag cagcgagtag
     1621 ctcgtgtaag gagcgtcgac ggtggcgctt cccacctgga agctgccgtc acgattgatc
     1681 ccgccatcat acgtttgctc acccgttctt gaacgcagga cgatattgag cgcgcctccg
     1741 gtctgccgca gcgtaaacaa atcaccctgg ctgccgtgca gtttggaaca cggcccaacg
     1801 cggcaattgc actccgtcac cgaggacttc gtgagcatgt acgcgccggt gaaggaagtt
     1861 cccgacgcgt caccggcagg tagccccgtc acgtcagcag gggataaagt acggggcggt
     1921 tcgtcggtcc ctccacagcc catcgacaga accagccccg caaccacggc gcacccgaca
     1981 cctgagtttc cgctcttcat ctcgactcgc gcgggagcat acgcccactc gtcaccgcac
     2041 gcccgcagtt cccgcgcagc ggactcgcta cacggctaga ttgcccgcgc atagcggccg
     2101 ggcgaagtac cgacgtgtcg actgaaggcc gtgctgaaag tgctcgaaga agcatagccc
     2161 acgcgctccg cgatgaggga gatgtcgagc tcccttcgcc gcaggagatc tttggctacg
     2221 gccatgcgcc aggtcagcag gtattccatg ggccgcatcc caacggctac atggaaccgc
     2281 tcgaagaacg aggaacgcga gagaccggca gccttggcaa gttcgggtac agtccaggca
     2341 tggccgagtt gatcatgcat cttgcgcaga gcgggcgtga gccgcgcatc ggccaaaccg
     2401 cgcaaaagcc ccgcgggcgc tccttctccc ggttgagaac gcagggcctc gatcagcaag
     2461 agctcgacca agcgcgacaa taccagttcg cgacccgcgt tctgcgctct cgcttcctcc
     2521 gcgaccagct gcaccaagag cgtgagacgc gggacgccgc gaatgtgaaa caagcccgga
     2581 agtagcgaca ccagtaacgc gcgatccggc gaaccgaagg taaaagaacc accgagcagc
     2641 cgcacgttgg gctttccgcc tattcggccg tgccgcactt cgctggtcgg caatggcacc
     2701 gacttgggat ctatgtgcac gggcgccgcc gcctcgaagc cagacatcgt gaagccgggg
     2761 gtcgccggca gcagtacgaa atcaccggcc tcgagggtta cgggacggtg accatcgacc
     2821 gccaatcgac agctgccctc gagcaccgcc gcaaagcttg gcgcgtcgaa gcgcgaataa
     2881 cgaaccgccc acttgcctgc cccgctgatc ggcttcgaga gcacggcccg aggctggagc
     2941 agctgaatca tctcggtgag ggggtcgccc atggtcgccg tgaccggact ctagcaaaca
     3001 aaacacgtac tggcacgggt agatagtcca actgcgagcc tctagattgc ctgcatgaaa
     3061 acgatcctca tcacgggctg ttcctctggt tatgggctgg aaacggcccg tcactttcac
     3121 gcccaaggtt ggcaggtgat cgccacgatg cgctgcccgc gttccgacat actgccctcg
     3181 agcgagcggc tgagggtgct gccgctcgat gtaacacgga gcgagagtat cgcggccgcg
     3241 ctcgcggaga gtggcccgat cgacgtcttg gtcaacaacg cggggattgg gctgttcggc
     3301 gcgctcgagg tcacgcccat ggccacgatt cgagagatct tcgagaccaa tacctttggg
     3361 gtgatggcga tgacccaggc ggtcgtgcct cgatttcggg cacgtcgctc gggcacgatc
     3421 gtgaacgtca cctccagtgc cacgctcgcg cccatgcccc tggtctcggt ctacaccgcg
     3481 agcaagatgg cgatcgaggg cttcacggcc tcgctcgcgt tcgagctcga ggcattcgac
     3541 gtgagggtca agctggtgga gcccggttac gggcccagca ctgctttcac gaagaacact
     3601 ggctcacgtc tagaagggct aattcccgag gcatacgcgc ccttggcgca ggagatcttc
     3661 gccgccctgg gccagggcac tggagcggtc acgaaggaga gcgacgttgc cgacgcgatc
     3721 tatcgaaccg tcaatgaccc ttcaaatcag ctccgctttc ccgcgggagc cgatgcaatc
     3781 gcgctcgcgc aaggcggctc accaaagccg caagctcacg gcggagagcc ctcttcgcga
     3841 gtgtagcgcg cctcgaccac cgggcgaccg tcgaccgcgc gtgtcatcaa ctcgatactg
     3901 ccgttcgccc agtgccactc gggccccggc cgcgtctcac cttgctgcag gcacgcccag
     3961 accgaatctg cgcacgccct cgagagcgcc gaaggctcgg tgttcgcatc tccaaacgtc
     4021 ctacgcattg ccccgttcaa ggcctcgtag tcccgctcca agcgctccgg actcgtgtag
     4081 tagcggagtg tgatcccaga cagggtgccc aaggcaaact cgaaccggaa gtcgggccga
     4141 gccatggttt tggtgcggtg cgagcagtaa gcgctcgcgc tctcggtgcg ccactctgcg
     4201 gctcgtatcg cgcaggctcg ttgggcctcg ggcagcgcac tcccaaactt gaactcgaag
     4261 acagactcag ggaagccagg gcggacaggt ggagcattgg ccttcgttgc cgcctcggtc
     4321 tctcgacgca atcgcgcaca cttgaactcc acataggtgc cgtaaatcgc tccaagtcca
     4381 aacgccacca tcgcgccgta gccgaatccg cgtgcgaccg cgtacctcga atcgctcgag
     4441 tgcgaatcgc gcgtgatggg ctgcccgaac tggtcgtagt ccatgctctg gttggcttct
     4501 tttctttcct gctggttgct ccagtgcacc caggtgtagc ccgcgatcac ggaagccgcg
     4561 tcggcgatgg gatataaggg attgccacat tgcttgtcgg ccttcgccgc tcgcttgctg
     4621 ggctccttgg gcggcaataa ttgagaaaac gagcaacccg ctggggagag gagcaggaaa
     4681 atagcgactg accctcgaac gaccttatga acgttggcaa ggcgacgatc ggcacgtgac
     4741 attcggagga ccctactctg ctcgttccgg ctccccgacg acaagacggt ctgggcttct
     4801 catcgcgccg ctccagtggg cgcgctggcc tggcgctccc atgttgcgat tcgatctttt
     4861 ttttcacgcc tcgcgaatcg aagctcctgt tggcggcctc taggtctgcg aggcccaaga
     4921 gcccatcgcg ccgagctgac ggaaaagccc atgggccgaa ccaaggagag aacgatgtct
     4981 gcggtaaaga gtgcgttttt cgtgaagaat gtaccgggat gggagcgcgc gctgcgcttg
     5041 gtgttggcgg caggcgccct cgtgttcggg gtggtgcagg gaggaatgtt gggtgcagtg
     5101 gggttcgggt cagcggccat gctggcgttc tcggcggtgc tcggcttctg cccaatgtgt
     5161 gcgctggcgg gccggcgcct cgcgagcaag ggttagccgg cgtggcgctc acggtatccc
     5221 ctgctctgtt cgatgcagct cgcgctggtg atgccggagc gatcggacgg ctcctcgagt
     5281 tatcgcgccc ggagttgcgg cgctacgccc agcgcagttg catcatcact gacgtcgagg
     5341 acgccgtaca ggaagcaatg tggacgctcg cccggtacgt gggtaccttg cagagtgttc
     5401 gcgccctgac cacctggttg ttccgggtca cgcgccgtga gtgccgacgt ttggcgcgca
     5461 agaccctagg gctggatctg tgggatgact cgaagaccga agccgtgatg gcgcggctgc
     5521 cgccaccgac cctgcgtctg gaggtggcag ccgccattca atctctgccc gagcactacc
     5581 gagacgtctt gctgctccgt gatttcgagg aactgaccat tgcggagctc gcagagcgac
     5641 ttgccttgtc cgaagccaac gtgaagagcc ggcttttccg agcgcgcgag ctagtgcgcg
     5701 agtacttgct gagcggtgga cctggagctc ccgcaggctc ctgaacggaa accgccagcc
     5761 ccacctcggg agcctagcca cggacttgct cctaccttgg ctcgatcata tctgcgttcg
     5821 cgaggctcaa aaaccgattc tcgatgcgac ggctttgcga atagtctgcg tgcccgcttc
     5881 gcggacagga ctgcagacgc gcaacgacga ttgcgcagag gaggatcgag atgaaccgtg
     5941 ccttacttca tacttcgggt gttgctttcc tcaccttctt ggtgaccgcc ggtctcgtct
     6001 cggggtgcgc gggcgaggca aactccagtg tgcaagagga gcccctcgtc attgaagcct
     6061 atgtcgaggg caccgtaacc agcggggtga cccatacccc gattgccggc gcgaccgtcg
     6121 cattgccggg ggtgatgggc gaggctggct caacgaccac cgacagcaac ggcttctata
     6181 gcttgaaggc gccgggggtt ggtgcgcaca ctttgttcgt gagtgcgaag ggctacgctc
     6241 gagccaaata cagcttctcg gccaataccg aacccggcgc ctccggccaa atccaggtga
     6301 cggcggtccg caactctcag ctctacccaa cgagcgacgc ggacggaggt gctttgagtg
     6361 ggcgcgtggc gagcggaggc gggcgtgacc tgatcgcggg cgccaaggtg ctggtgcagt
     6421 ttctggacac gagcgatccg atcgaggacg cgagcttgaa ggtgacggcg caaaccgact
     6481 cggacggcaa gttcatgctg aagaacttgc ctgccggcgc cccgcagacc cgggtgacgg
     6541 tgctccctgc agacgtagac gacgacggca agccggacac ggcggtgttc agtaacttcg
     6601 tttcgggtgg cccaagcaat ggagtgctca cgccgggcgg gcagaatttc atggatatcg
     6661 tggtggaccc gtacattggt gacaaagtgc tgtggaccaa cctggacgat cgacccagtc
     6721 tcgatccaga cgaagagatc gtggtcgcct acgccgagag catggtcgac acgaaggacg
     6781 caaccctggt cacactcgcg gaaggagcag atcgggtaaa ggtcgacttc gagtgggaag
     6841 acaacctgaa gttgacggtg acgccgctcg aacctctgct ccaagggcac acttacacgc
     6901 tctcggtgca gacgcagagc acggcgggcg tacccgtcgc gttgagccgc tcgttcctgg
     6961 tcacgagcgc cgaggctccc cccgacgccg tcacggggct cgagattgtc gacgcggatc
     7021 cggtgccgtg gagcacgcgg cgcttcacgc tggcgtggga cgctgcgtcc gatgtagatg
     7081 gcaacccgat tgatacctat cgagttctgg cgcgcaacga cgcgcgtcaa accacctgga
     7141 cgattctgtt cgagggcgcg gtgagccggt tttcccgccc gcaactccag gtcaacctac
     7201 ccgagagctt cgacagcttc cccaacgaag atgtgttcag cgccgtcggc ttcggcacca
     7261 ccgtggagtt cgcggtgcag gcctacagcg ggcgcaatga cggccccatc tcggaagaac
     7321 ccgcaacgtt ccaagacacg cattgtcctt cgctcaccgt cactccgccc gtgaccaccg
     7381 acaacagcct cggcaccgag gagctcgtgg tcaagtttct catttcgagc aacgacaacg
     7441 agccgctatc cgacgattcc gagcccaagt tcacttttgc cgacggcacg ctctcgacag
     7501 accccttcgt gatcagcggc gcacctcggg tccggcgcgt ccgggcgggc cagctcgagt
     7561 actcgttcgc cattcccaaa ggaaagaccg gcgcgggcga cacactgacg ctcgacctca
     7621 gcaaggtgac cgacacctcc ggcaacgggc ccgacggctc cgccggttgc gtgaagacgc
     7681 aaagtcttac gctgaactga gcctgctttt cgcgtctcgt tcgttcccgg aaaccgaggc
     7741 tgagg
//
LOCUS       DASTKU010000580        20393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1004575, whole genome shotgun sequence.
ACCESSION   DASTKU010000580 DASTKU010000000
VERSION     DASTKU010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 20393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20393
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004575"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VFQ61_20200"
     CDS             <1..1100
                     /locus_tag="VFQ61_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956834.1"
                     /translation="LSQVGCPMLEPISGVPVAWALFSGGPTSTYICQVLRGACVYFRR
                     VPLQEPNLGPAGLVEKIQGRVPGILLYGIAPPKRDTLPTRLVEIAAQQRARLQQLQPD
                     GLIVYDIQDESERTPEPRPFPFVRTLAPEQYAYETLAELGLPKIVYRQVTHGAPAEFR
                     AFLERVRTRAEPSLSVLVGAPTRRTEQIQLRLSEAYALARESAFAEKLVLGGIAIAER
                     HQARFDEHQKMLAKSEQGVRFFVTQAVYDVASSKSLLSDYAIELSQREQAPVPVLLTF
                     SPCGSEKTLAFMKWLGISIPRWLENELRYSTDILEQSVELCVQIFQELWLFARKKGIP
                     LGANVESVSIRKSEIDASVELFQRLRRIMRG"
     gene            complement(1155..2024)
                     /locus_tag="VFQ61_20205"
     CDS             complement(1155..2024)
                     /locus_tag="VFQ61_20205"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET9956835.1"
                     /translation="MTPPSRPSASPAPLGAFGMSRAKPSGKVVAVASPKGGCGKTTVA
                     LNLALSLARQGRTVALVDADINGDVLSTINARKRAEVGTFDVLLGIARPEEALLPTIL
                     PNFKILPAVGGQLPRADRLNADHTPAFRTLLSGLSNQFDVVLVDTPAGMFGVTYQVLV
                     SATHVLAVLQAEAVASRSFPRFVEGLRAIDDEDRPEVLGVVVNMLQTKHTASLAVFQN
                     ACNDLPGEWLFDTAIPRHRAFLDATHVGLPLRHMDDQAPPAVAWLFDNLAGEVADRLR
                     LGAVEKKPQPLLL"
     gene            2293..3756
                     /locus_tag="VFQ61_20210"
     CDS             2293..3756
                     /locus_tag="VFQ61_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9956836.1"
                     /translation="MQIELIAPASEDSAFLPRLGLGVLAALTPPEDEVIYTDDLVRPF
                     DLERDIKDVDLVGISVDSKTARRSYAIASEYRRRGVKVVLGGIHPTALPEEAANFADA
                     VVLSEAENLWPQVVADAKRGALQRIYSGPLPDLAGKPNARRDLFRSKKYIPFQVVQTM
                     RGCPYPCEFCSVSTANGTTMRFRPVDEVLNELRSLGKLVMFADDNVMIHRKYSGELFT
                     RMAELNKHWIGQCSLAAVKRIENIRLMAQSGCKALFIGFESIDDKTLQHTGKRQNRPS
                     EYREVLDMLHDHGISTWGSFVFGFDTDDPEVFDRTVEFGIEMRLTMALYAILTPYPGT
                     KLYKRLLAEGRLTDPRWWLRENHDQGSPYFIPTRMSREALREGWQRAWQRFYSPSSIW
                     KRWTVRTGSSWIQTLGYLPINAMQNRLVKHKIMGHKPRFRSAEFDPMSQAFDAIRALS
                     ERGDPMPEARAEAAGLISSARLTKSANSAQEQTGPNT"
     gene            4022..4864
                     /locus_tag="VFQ61_20215"
     CDS             4022..4864
                     /locus_tag="VFQ61_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9956837.1"
                     /translation="MRFALITDVHFGPRAYHEGKLRKMSERAPELTARFVERMNAQER
                     PELVFNLGDVIEDESRALDLKRYREFAQILAGVSGRVQHVPGNHDQVYLTREDLLSIW
                     GHDAERVAKGELSNCSELYYSFDFGGLHFLVLRTDEYKDHHIELPEVQMEFAERDLGA
                     TRLPTLVLMHHPASEQRLSGNRWFEKAPHVCRVRERGRLRRIFESSGRVVAVFNGHVH
                     WNHLDVIAGIPYITLQSLTENLDEDAPGRPASAYAVCDIDARSLHVDIRGEEPLRFQL
                     ELPR"
     gene            complement(4885..5988)
                     /locus_tag="VFQ61_20220"
     CDS             complement(4885..5988)
                     /locus_tag="VFQ61_20220"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9956838.1"
                     /translation="MLFRQLFDQESSTYTYLLADQASRKAVIIDPVFEQVERDATLIR
                     ELDLELLFALDTHVHADHVTGSGLLKQRLGAKTVLSERAGAVCADKLVKHGDQIVFGG
                     SRLEIRETPGHTNGCLSYVSHESARVFTGDALLIRGSGRTDFQQGSSDLLYRSVIEQI
                     FTLPDETEVFPGHDYKGRSSSSIAEEKRWNPRLGQGKSAQEFATIMRELKLPHPRKMD
                     IAVPANLYCGVRPDSSRPRAEADTSFAPVVISGSGVPELGSEWVAGNAGDLTVLDVRE
                     PDEFLGELGHLPTAELVPLAALAARAASLPRERPLLTVCRSGGRSGQAALLLLQHGFT
                     RVASLRGGMLDWSARRLPVEFGAAPSHVTNRQG"
     gene            complement(6002..6502)
                     /locus_tag="VFQ61_20225"
     CDS             complement(6002..6502)
                     /locus_tag="VFQ61_20225"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HET9956839.1"
                     /translation="MTRRLLASLAAFGSGLVFGAGLVLGGMTDPNKVLGFLDVTGDFN
                     PTLLFVMAGAVSVHALAYRVVRKRQTPWLAPKFVLPTRRDIDWKLIVGSALFGLGWGL
                     AGYCPGPALVSLGSGSSAWVFVLSMALGSYAAARFESWLARPALQAAVRSADSDLTHP
                     PLQGGA"
     gene            complement(6499..7008)
                     /locus_tag="VFQ61_20230"
     CDS             complement(6499..7008)
                     /locus_tag="VFQ61_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HET9956840.1"
                     /translation="MISILREKLPICTWHVICNEGCKGDIVLMQDVYVVRALFGGALI
                     GVSASALLLGLGRVAGISGIFGNLVIGPRTDVGFRVAFLLGLISAGLAAVWFAPALLG
                     ASPRSLPWVALAGLLVGVGTRLGSGCTSGHGVCGISRLSFRSLLATLTFIVTGALGVL
                     LYRMIGVQP"
     gene            7143..8081
                     /locus_tag="VFQ61_20235"
     CDS             7143..8081
                     /locus_tag="VFQ61_20235"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9956841.1"
                     /translation="MFLLSVLLASMVGIALGLLGGGGSILTVPILRYALGMPAHAAIA
                     TSLLVVGVTSVVALVPYATRGKVQFKVGLLFGSAGMVGAFLAGRVAHHVPPTLLLIAF
                     AAMMAATAFAMIRGQAKPARATSELPRSRAPRWVWLKILAEGLLVGAVTGLIGAGGGF
                     LVVPALVVLGGLPMDLAIGTSLVVISMKSIAGVIGFWGHTPVDFRLALPIAAAAVLGS
                     ILGGRWARHIEAAALRRMFGVFVIAMACFILFQELPPLLGVRPRILISIVAAAGLTSL
                     FSLAMRLWERVRVETFVTPSKIVARPRSRPPGPQPR"
     gene            8219..9745
                     /locus_tag="VFQ61_20240"
     CDS             8219..9745
                     /locus_tag="VFQ61_20240"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET9956842.1"
                     /translation="MQVPPPDPPLEVESRSPGEALDRATLQRRAKWGVLLLAGRTVLQ
                     QLIILVGNVYLARLLGPREFGAFWIIQFAISFFTLFGDAGFGAALIQKKESATQEELS
                     SVFWMQMLMGLAVIGLVFLAAPAVTVVWPALPAGAPAMLRGLSASLLLTAMRVIPTIL
                     MERELLFGRLSFIDLVLTVSFYGTAVVLAHQGYGSASLVLGMLAQGVAALVAAFVLRP
                     WRPGMHLNVSTLRPILRFGLTFQAKHIVGFVNGAVMPLYAGRALGPYALGLVTWSQTT
                     AFFPVKLVEILGRVNFPLLSRLQHDPEALARTLEKTLQVCLIVTMLFVSLILGLGPSI
                     VQIIYGDKWVPALPTLYVFTFAIAIGFVSPVMNSALDAIGQPRVMLKLALGWTLLNWL
                     VVVTTMQFMRSALVFALACSVHVLVGNLAVILVVKQQIPGVRLWAQSRAGVGAAIAGA
                     CFGRWLLLSRIAGPFSLIAAVLVVAAVFTAVLAVLDRSAAIALLALVQRRSKSASRAA
                     "
     gene            9742..10422
                     /locus_tag="VFQ61_20245"
     CDS             9742..10422
                     /locus_tag="VFQ61_20245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9956843.1"
                     /translation="MSDAPVPNDYYEHPRPEVVDCVPLSARNVLDVGCASGALGHSLK
                     LARPEVQVRGVEPVAAQAERARAVLDDVLHGGADDPLPSHWPAPECVIFADVLEHLVD
                     PWSTLRRYRSILKPGGTLVASIPNVAHRVVLRGLFRMRWDYTDFGILDRTHLRFFTRD
                     TAVELVESCGFQIRSIGRTTEGLGRGLFSKKVRDVLAKENGSRRVFPRPLAWLIDAYT
                     VQFLIVAE"
     gene            10451..11491
                     /locus_tag="VFQ61_20250"
     CDS             10451..11491
                     /locus_tag="VFQ61_20250"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9956844.1"
                     /translation="MRFFWTDEDRPARRRGSALRAALAYAGHSVHRGVIGDEPPPGTD
                     VWIYGLGVVGAPAIEPRVVGQLLDAQAAVVLTQLCDAESMCFERIPHELAIRARLFLR
                     NHWPSDSHSIPAAFRDRIGWAPPMIKSMPARPGRLLRERSHGAIFFGTRTGFSNMPGN
                     KNAREEVVRILRASGLPFLGGLLHHGAECYRTEPSLLVPRLRERTHLKHLANSKICLA
                     PWGNHRLTYRLFEGMAMRCLVLAQPIRSTRFLDAGLEPGTHYVEIAPDLSDLADAVRY
                     YLNHLDEAQCIADAGHEHFKKYFAARGPLISKWIFDSVVGSWGDLYRSAAPRRARSVA
                     IAAAARLRPSAF"
     gene            11600..13132
                     /locus_tag="VFQ61_20255"
     CDS             11600..13132
                     /locus_tag="VFQ61_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956845.1"
                     /translation="MQKRWAFIALLVAAACGLQFIGCRGNLNVYDEGLALTGAMRVAR
                     GELPYRDFWTIYSPGQFYLLAAFMRFTGEGLIVLRHFYAAILAALAVSSAVLGGSLSR
                     GSSSRRLTVTSILLVISATLCTSYRHYFHAAPVALLMAMLVLLLGAQPSPRRFFPAGL
                     LTGLAVLFRHDIGVYLFGSVASVLLLYPQDRTRRLAPLGWYVAGSCVLPASCFGALVS
                     AGAGSAMWDQLVVFPLTVYPGVRSLPWPLFQGSWSFPRVVETLATNAVILLFAYQFWR
                     LLGLWRDSHRAHSAPDSRIVMMFAFGVACLLLAKTRPDFAHVFPSAMLALSLTPALLS
                     EFSVGSKVLALVVATLFALTGVSASWRQWRASNGYCLSSGVGRGVCGGDATREYDEVI
                     ARLGVLTRPRERIYVGEAVHDRMLTSDAALYYLAGRMPACPWHELHPGEATKGTVQTA
                     IAECLESTKVIVLRQSKPSREPNLSSKPTGVDTLDRFIKTRYLPLDTFERYSILTREL
                     QR"
     gene            13250..14596
                     /locus_tag="VFQ61_20260"
     CDS             13250..14596
                     /locus_tag="VFQ61_20260"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9956846.1"
                     /translation="MPRLEPAKSAASLIAQALSLAVGPALVLDGDLRVLLATADAARV
                     LGFDVPLGASAPKLLCRNSPKREVAAAMSQGLAIQTTIAHPADDAGERRLKLRALPLG
                     APPNAGFLLMLDEAPSSSSEPVLFQGMWTENARLKEVFRIIERVAVDDATVLVRGETG
                     TGKELVARAIHALSPRAGGPFQAINCAALPANLLESELFGHVRGAFTGADRDTKGHLQ
                     LAHKGTLFLDEVAEMPLELQPKLLRVLETRSVLPVGGRTPVPIDVRVISATHRALRRE
                     VELNRFRADLMYRLRVIPIFLPALRERKEDIALLIEKLCESMNKAARRKVERIAPSAL
                     ALLERHDWPGNIRELRNVIAYAYAIGEGPILQIKDLPIELVDAGGPSEGAQASERLRE
                     LAARDPEVRRILQALEKAGGRRHRAAKILGISRVTLWRRMRELGLDEDEPSAEPRD"
     gene            complement(14627..15955)
                     /locus_tag="VFQ61_20265"
     CDS             complement(14627..15955)
                     /locus_tag="VFQ61_20265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956847.1"
                     /translation="MAALFSGCQGSAATGELQPAHFEAGASCEAQKSELIQYIGALSP
                     NPLTPNIGADLPESALGRPPRMGVHLEIGETGAQLDGRPLQAPAQAALAAALKDTSRN
                     ALLYVVAPAATDVRTVRSYLRAVPTGMAVELLVKVPEVVPGEPKASAAASRGSDNSFA
                     GQLLRERDPARRAELALRGYRQYSKCPAIEQAAAAIPSTPAEQRWPNLQKGLLAALPG
                     CGCQTLDTQNLKQIAIAEQRAGSMSLGSLPIGFLRDERCGASMPLRSMRKLVTQMESF
                     DAEFAGTFQKDELTFTSVLENERLLNFFCDALPGETFAALSRERRTLYFKLANTERCE
                     AMRLEPLSPGAPMGTAWRASPSGSALGFHYWQGAEEIRFYGPLQAGAASSPTDTRDWA
                     CNQNVRLSSVDARSIGLEGGRWFFDEEECRKSPAEGNLFTGCAGNAPSPP"
     gene            complement(16216..16950)
                     /locus_tag="VFQ61_20270"
     CDS             complement(16216..16950)
                     /locus_tag="VFQ61_20270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956848.1"
                     /translation="MGKPVLPCAENRTHTENPARKTPMRVKQVKVRVHRSNARSDARR
                     STADSSSVLGHLPESQLASVKPAEPVPGGCRRSDNPQRRNRQTNPDMQRIWLTALLSI
                     VCTLGVACGPGSDGHSNATGGLGGASATQEQTTTVSGGTGNVNGSTCTEGSGSTDCSG
                     NVGNGQGGGSQTMPAAPAAGQAGSEDPPNDENKTRTLEPCCESGITTLCLCVVGETCP
                     GATGVMFFSDGTCCDPRDISNCADGP"
     gene            complement(17253..18368)
                     /locus_tag="VFQ61_20275"
     CDS             complement(17253..18368)
                     /locus_tag="VFQ61_20275"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET9956849.1"
                     /translation="MTEPSVTTCPPSHRPPPPVQLIPSTLHALAASNQPKTARSAPVQ
                     ANPRRSARPKQAFGIWHYLGLSAVAAATSLGLFAYAHSRGEHGTSRVPPDSAHPATAS
                     LKQSAKGHPVRWQHADVVVALDDSIDTLGPTARNSIKEAFTAWTKAGVQLPNPHFERT
                     SKVSAEPKPDGVNSVTVAPITIAGHEHDLAITLTYSDEDTGEIVEADIVINSLYPYRV
                     LNQQSENAVTASGKGNGSQAGGEDSATAAHISSSHASCTSEARNVQCEQQSYDVQNVV
                     THEVGHFFGLGEDMTDASATMYYCTNRCETHKRELTSGDMSAVTELYQAGFVNEAAGC
                     GGAHFAARKPTWLPFLGVSLGLLLVTRGRRASRRRSA"
     gene            18458..19996
                     /locus_tag="VFQ61_20280"
     CDS             18458..19996
                     /locus_tag="VFQ61_20280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9956850.1"
                     /translation="MLSEATRRLIHERLSAEVGRIDKEAPARVALCYPSPYTVALSSL
                     GFQRVYAAIQAMPGVMCERAFLPDDAALPAARVLERPVTYEHRRELSSFPVLALSVAY
                     ELELAGLVKLLESSGIPALAQERSERHPFVLAGGPLTFSNPQPLSVFADAIVMGEAEE
                     LVAPVLQAIFSAGSRAEACEALANIPGVFVPSIHGESHPKPAACDDALLPAHSTIRTP
                     HTELSDMFLIEVERGCSRGCTYCVMRRSTNGGMRIVPKEVVLEKVPADARRVGLVGAA
                     VSDHPKITEIVRGLAERGVQVGLSSLRPDRLKEEFISALKAGGYRTITTALDGSSERL
                     RGIIERRGREPNYVAAAELCRKYGMDRLKLYLMVGLPGETDQDIDECVRFVGELSRII
                     PIALGVAPFCAKRNTPLDRMPYAGVNVVQSRLERLRRGLRGRADVRATSAKWAWIEYV
                     LSQGGTAEGLAVYEAVRAGGGYAEYRRAFAKLGHSPDGRGYAEVVPPIAPERAKMRRL
                     SLLG"
BASE COUNT         3575 a         6577 c         6364 g         3877 t
ORIGIN      
        1 aactgtctca ggtcggctgt ccgatgctcg agccaatcag cggagtaccc gtagcttggg
       61 cgctcttcag cggcggaccg acctcaacat acatttgtca ggtcctccgg ggagcatgcg
      121 tgtatttcag gagggtgccg cttcaagagc ccaatttagg ccccgcgggt ttggtcgaga
      181 agatccaggg gcgggtccca ggcattctgc tctatggcat cgctccgccc aaacgcgaca
      241 cgctgcccac tcggctagtc gagatcgcgg cccagcaacg agcccgcctc cagcaactcc
      301 agccggacgg gctgatcgtc tacgacatcc aggacgagtc cgagcggacc cccgagccgc
      361 gccccttccc gttcgtgcgc acgctcgccc cggaacagta cgcctacgaa accttggccg
      421 agctggggct gcccaagatc gtctaccgcc aggtgactca cggcgccccc gcagagttcc
      481 gcgcgttctt ggagcgagtg cgtacccgcg ccgagccaag cctctccgtg ctcgtgggcg
      541 cgccgacacg ccgcaccgag cagatccagc ttcgcctctc ggaagcctat gcgttggcgc
      601 gcgagagcgc cttcgccgag aagctcgtgc tgggcggcat cgcgatcgcc gagcgtcacc
      661 aagctcgctt cgacgaacac cagaagatgc tcgccaagag tgagcaaggg gtgcgcttct
      721 tcgtcacgca ggcggtctac gacgtcgcct ccagcaaatc gttgctctcc gattacgcca
      781 tcgaactctc gcagcgtgag caagcaccag ttccggtttt gctcaccttc tccccgtgcg
      841 gctccgagaa gacgctcgcc ttcatgaagt ggctgggcat ctcgatcccg cgctggttgg
      901 agaacgagct acgctactcg accgacatcc tcgagcaatc ggtcgagctg tgcgtgcaga
      961 tcttccaaga gctttggctg ttcgcgcgta agaagggcat cccgctcggc gccaacgtgg
     1021 agagcgtctc cattcgcaag agcgaaatcg acgcttcggt cgagctgttc caacggctgc
     1081 gccgcatcat gcgcggttga tcgcgcgctc gcgagccaca cgcccaccca cgctggcgca
     1141 aacccgctag gaaatcagag cagcaggggc tgcggctttt tctcgacggc cccgagcctc
     1201 agccggtccg ccacttcacc ggcgaggttg tcgaataacc aagccaccgc cggcggcgcc
     1261 tgatcatcca tgtggcgcag cggcaggccc acgtgcgtcg catccaagaa cgcgcgatgt
     1321 cgcgggatcg ccgtatcgaa cagccactcg ccgggcaaat cattgcaggc gttttggaac
     1381 acggccaaag acgcggtgtg cttggtctgc agcatgttca ccaccacccc gagcacctcc
     1441 ggccgatctt catcgtcgat cgcgcggagc ccttccacga aacgcgggaa agagcggctc
     1501 gctacggctt cggcttgcag cacggccaac acatgcgtcg cggagaccaa cacctgataa
     1561 gtcaccccga acatccccgc tggcgtatcc accagcacca cgtcgaactg attactgagc
     1621 ccactcaaga gcgttcggaa cgccggggtg tgatcggcgt tcaaacgatc ggcacgcggc
     1681 aactgcccgc ccacggccgg caaaatcttg aaattcggca ggatcgtggg gagtaacgcc
     1741 tcctcgggcc tcgcgatgcc gagcagcacg tcgaacgtgc ccacctccgc gcgctttcgc
     1801 gcattgatgg tggagagaac atccccattg atgtcggcgt ccacgagcgc cacggtgcga
     1861 ccttgtcggg ccaagctgag agcgagattc agcgccaccg tcgtcttgcc acagccacct
     1921 ttggggctgg cgacagcgac cacctttccg ctgggcttcg cgcgcgacat gccaaaagca
     1981 ccgagcggtg cgggagaagc ggaggggcga gaaggagggg tcatcgcgat ttcgtcgaga
     2041 gcttactcaa cacagcctca tagcacgtaa gcttttttga gacccaccct cggggcaagc
     2101 gcccacgcgg gttggaatgc caaaacccgc cgccaaaaag agacagcgcc gagaagccaa
     2161 gcgcttcgag cacgcagagc cgcaaaggtc tcgttcccgg atagtctcga tcttcggccc
     2221 ccttacctcc gtccgtacac tcgaacttgc ccgagcgccg cgcggcgtgt agcgcttgaa
     2281 tgagagctgc ccatgcaaat cgagctgatt gcacccgcca gcgaagactc tgcgttcctt
     2341 ccgcgcttgg gcctcggggt cttggccgcg ctcactccac ccgaagacga ggtgatttac
     2401 acggacgact tggtgcgtcc gttcgatctc gagcgcgaca tcaaagacgt ggacttggtg
     2461 ggcatcagcg tcgacagcaa gaccgcgcgg cgcagctacg ccatcgcgtc cgagtaccgc
     2521 cggcgcgggg tgaaggtcgt gctcggcgga attcacccca ccgcgctgcc ggaagaggcc
     2581 gccaatttcg cggatgcggt ggtgctcagc gaggcggaga acttgtggcc ccaagtcgtc
     2641 gccgacgcca agcggggggc tctgcagcgc atttactcgg gtccgctccc ggatttggca
     2701 ggcaaaccca acgcgcgccg cgatctattc cgctcgaaga agtacatccc ctttcaagtc
     2761 gtccagacca tgcgcggctg cccctatccc tgcgagtttt gcagcgtctc caccgcgaac
     2821 ggcaccacca tgcgctttcg ccccgtggac gaggtgctga acgaactccg atcgctcggc
     2881 aagctcgtga tgttcgcgga cgacaacgtg atgattcacc gcaaatactc cggtgaactc
     2941 ttcacgcgca tggccgagct caacaagcat tggatcgggc agtgctcgct cgcggcggtg
     3001 aagcggatcg agaacatccg tttgatggcg cagagcggct gcaaggccct cttcatcggc
     3061 ttcgaaagca tcgacgacaa gactttacag cacaccggca aacgccagaa tcgcccgtcg
     3121 gagtaccgtg aagtgctcga catgctgcac gaccacggca tctccacctg gggcagcttc
     3181 gtgttcggct tcgacaccga cgaccccgag gtcttcgatc gcaccgtcga attcggcatc
     3241 gaaatgcggc tcaccatggc cctgtacgcg atcctcacgc cgtacccggg caccaagctc
     3301 tacaaacgtc tcctcgccga agggcgcctt acggatccgc gctggtggct gcgggaaaac
     3361 cacgatcaag gttctcctta cttcatcccc acgcgcatga gccgcgaagc cctccgcgaa
     3421 ggttggcagc gcgcttggca acgcttctac tccccgagct cgatttggaa gcgctggacg
     3481 gtgcgcaccg gctcgagctg gatccagacc ctcggctatc tgcccatcaa tgccatgcaa
     3541 aatcgcttgg taaaacacaa aatcatgggt cacaaaccgc gcttccgaag cgccgagttc
     3601 gaccccatga gccaagcctt cgacgccatc cgcgccttgt ccgagcgggg tgacccgatg
     3661 cccgaagcac gcgccgaagc cgctggcctc atctcgagtg cccgcctgac gaaatccgcg
     3721 aactccgcgc aagagcagac cggcccgaat acgtgacggt ccgtgctgcc aagtgcgagt
     3781 ctgcagtgcg cgccgacgat gggtccaatc gccgttcagt cgctcgagcg aatcgtgatt
     3841 tcttggccgc tccgttacga gcgcgaaatc cgtgtgagcg cgcgccagcg cagcttcttc
     3901 gacccattca gcatcgcgtc accgcgcagg cccccgcaat aacgcgagcc tgcggcgcca
     3961 cccgtctcgc cgggggcaat cttgccaggc ccggtcctcg gggatatagc cgcttttgac
     4021 catgcgtttt gcgttgatta cggatgtgca ctttgggccg cgcgcttacc acgaaggcaa
     4081 attgcgaaag atgagcgagc gcgcgcccga gctgacggcg cgtttcgtcg agcgcatgaa
     4141 tgcccaggag cggcccgagt tggtgttcaa cttgggggac gtgatcgaag acgagagtcg
     4201 agcgctcgat ctgaagcgct atcgcgagtt cgcccagatt ttggccggag tttccgggcg
     4261 cgtccaacac gtgcccggaa atcacgacca ggtatacctg acgcgcgaag acctgctgag
     4321 catctggggc catgacgccg agcgcgtcgc aaaaggcgag ctctccaatt gttcggagct
     4381 ctattacagc ttcgacttcg gagggctgca ctttttggtg ctgcggacgg acgagtacaa
     4441 ggaccatcat atcgagttgc ccgaagttca aatggagttt gccgagcggg acctcggcgc
     4501 gacgcgcttg cccactctgg tgctgatgca tcatccggcc agtgaacaac gcttgtccgg
     4561 caatcgctgg ttcgagaagg ctccccacgt gtgccgcgtg cgggaacgcg gtcgtttgcg
     4621 ccgaatcttc gagagttcgg ggcgcgtcgt ggccgttttc aacgggcacg tgcactggaa
     4681 tcacctcgac gtgatcgcgg gcattccgta catcaccctg caaagcctga ccgagaatct
     4741 agatgaggac gcgccggggc ggcccgcctc cgcctatgca gtttgcgata tcgacgctcg
     4801 cagtctgcac gtcgatatcc gcggcgaaga gccgctacgc ttccagctcg aactcccgcg
     4861 ctgattacgg gtcgtgaggt cccgtcagcc ttggcgattg gtgacgtggg acggggccgc
     4921 gccgaactcg acgggcaaac ggcgtgcgct ccaatccaac attccgcccc gcagtgaagc
     4981 cacgcgcgtg aacccgtgct ggagcagtaa tagcgctgcc tgcccggaac gacccccgga
     5041 acgacaaacc gtcaagagcg gccgctctcg cggcagcgaa gccgcgcgcg cggcgagcgc
     5101 cgcgagcggt accagctctg ccgtgggcag atgaccgagc tcgcccagga attcgtcggg
     5161 ctcacgcacg tccaggaccg tgagatctcc tgcgtttccc gcgacccact ccgagccgag
     5221 ctccggcaca ccggagccgg atatcaccac cggggcaaag ctcgtgtcgg cctctgctcg
     5281 gggtcgcgac gaatccggcc gaactccgca gtacaagttg gcgggtacgg cgatatccat
     5341 tttgcgcggg tggggcagct tcagttcgcg catgatggtc gcgaattctt gcgcgctctt
     5401 gccttgcccg aggcgcggat tccagcgctt ctcttcggcg atgctgctcg aactccgacc
     5461 cttgtagtcg tggcccggga acacctcggt ctcgtcaggt aacgtgaata tctgttcgat
     5521 gaccgagcgg tagagcaggt ccgagctgcc ctgctgaaag tccgtgcgtc cagagccgcg
     5581 aatcaggagc gcgtccccgg taaaaacccg ggccgattca tggctcacgt agctcaagca
     5641 gccgttcgtg tgcccggggg tttccctaat ctcgagcctg gagccgccga aaacgatctg
     5701 atccccatgt ttgaccagct tgtcggcgca aacggcgccg gcgcgctccg agagcacggt
     5761 cttcgcgccg aggcgttgtt tcaacaaacc ggagccagta acgtgatcgg cgtgaacatg
     5821 ggtgtcgagc gcaaacagca actcgagatc gagctcgcga atcagggtcg cgtctcgctc
     5881 cacttgctcg aacaccggat cgatgattac cgctttccgg ctggcctgat ccgctagcaa
     5941 ataggtgtaa gtggacgact cctgatcgaa cagttgacgg aataacatga ctcttccttt
     6001 ctcatgcgcc cccttggagc ggggggtggg tcaaatctga atctgcggag cggaccgccg
     6061 cttggagcgc aggccgcgcg agccaagact cgaatctggc ggcggcgtag ctgccgaggg
     6121 ccatgctcag cacgaacacc cacgcgctgc tgccactgcc gagggatacc aaggcgggcc
     6181 cggggcaata tccggccagc ccccacccca gcccgaacag cgcggaaccc acgatcagct
     6241 tccagtcgat gtcacgacgc gtgggcaaca cgaacttcgg cgccagccaa ggtgtctggc
     6301 gcttccggac cacacgatac gcgagggcgt gaaccgatac ggcgcccgcc atcacgaaca
     6361 acagcgtcgg attgaagtct ccggtcacgt ccagaaaccc aagcaccttg ttgggatcgg
     6421 tcatgccgcc gagcacgagt cccgccccga acaccagtcc cgagccaaat gccgcgaggc
     6481 tcgcaagcaa acgccgcgtc atggctgcac tccaatcatg cgatagagca gcacgccgag
     6541 ggcacccgtc acgatgaagg tgagcgtcgc gagcagcgat cgaaaggaca agcggctgat
     6601 cccgcatacg ccgtgaccac tcgtgcaccc gctgcccaag cgcgtgccca cgccgaccaa
     6661 gagccccgct agcgccaccc agggcaacga gcgcgggctc gcgccgagaa gcgcgggcgc
     6721 gaaccacacc gcggccagcc cggcactgat caaacccaac aagaatgcga cccgaaagcc
     6781 cacatccgtt cgcggaccga tcacgaggtt gccgaagatt ccgctgatac ccgcgacgcg
     6841 acccaagccg agcagcaacg cagaggccga aacaccaatc agcgccccac cgaagagggc
     6901 gcgcacgaca tagacatctt gcatgaggac gatgtctcct ttgcagcctt cattgcagat
     6961 cacgtgccag gtgcaaatgg gaagtttctc gcgaaggatc gagatcacgg gcgtgccctg
     7021 aaacgaaagc gttgcacgct gtttcggcac cgtgtttcga gcggagctcc gaatgccgcg
     7081 attcccgcag aaaagtgcgc ggcacgtacg tcgcattggc gcacctcagg agccgagtcg
     7141 cgatgttttt attgtctgta ttgctcgcat cgatggtcgg gatagcgctg gggttattgg
     7201 gcggtggcgg atcgatcctg acggttccga ttctccgcta cgcgctgggc atgccggctc
     7261 acgcggcgat cgccacgtcg ctcctcgtgg tcggggtaac gagcgtggtc gcgttggttc
     7321 cttacgccac ccgcggcaag gtgcagttca aagtggggtt gttgtttgga agcgcgggaa
     7381 tggtcggcgc gttcctcgcc ggtcgcgtcg cacaccacgt gccccccacg ctgctgctga
     7441 tcgcctttgc cgcgatgatg gcggccacgg cttttgcaat gatccggggc caagccaagc
     7501 ccgcgcgcgc cacttcggag cttccgcggt cgcgcgctcc acgctgggtg tggctgaaga
     7561 tattggcgga aggcctcctg gttggcgcgg tcacgggctt gattggtgcc gggggagggt
     7621 ttctagtcgt ccctgcactc gtggtgctcg ggggcttacc gatggacctc gcgatcggta
     7681 cttcgctggt ggtgatcagt atgaagtcga tcgcgggcgt gatcgggttt tggggtcaca
     7741 cgcccgtcga tttcaggctt gcgctgccga tcgccgcggc ggccgtactc ggcagcattc
     7801 tcggcggacg atgggcgcgt cacatcgaag cggcggccct gcgacgtatg ttcggcgtgt
     7861 tcgtgatcgc gatggcctgt ttcatcctgt tccaggagtt gccgccgttg ctcggcgtga
     7921 ggccccgcat cctgatcagt atcgttgcgg ctgctggcct aaccagcctg ttctcgctcg
     7981 cgatgcgcct gtgggagcgg gtacgcgtcg aaacgttcgt aacgccttcc aaaatcgtag
     8041 cgcgcccccg atcgcgcccg ccgggccccc agcctcgctg aatcccggtc aagaaatggc
     8101 agaggctcgg acgtcgggtg tcgcggaatc cgcggattcc cgtcgacata tcaactccgc
     8161 ggggccacct gccgccctgg ccaattgcca gaacggaaat ctcaactagc ttcgccgcgt
     8221 gcaggtgccc ccgcccgatc cgccgctcga agtcgaatcc cgttctcccg gagaggcgct
     8281 ggatcgcgcc accctgcagc gccgcgccaa atggggagtg ttgctcctcg cggggcgaac
     8341 ggtgctgcag caactcataa tcctggttgg caacgtctac ttggcgcggt tgctcgggcc
     8401 tcgagagttc ggggccttct ggatcattca gtttgcgatc tctttcttca cgttgttcgg
     8461 agacgcgggc ttcggcgccg cgctgatcca aaagaaagag tccgccacgc aggaagagct
     8521 ctccagcgtc ttctggatgc agatgctgat ggggctcgcc gtcatagggc tggttttcct
     8581 ggctgcgccc gccgtcaccg tggtctggcc cgcgttaccc gcgggggccc cggcgatgct
     8641 acgcggccta tcagcgtctc ttttgctgac ggcgatgagg gtcatcccga ccattctgat
     8701 ggagcgagag ttgctgttcg gccggctctc cttcatcgac ttggtgctca ccgtgtcctt
     8761 ctacggcacc gccgtcgtac ttgcgcatca gggttacggc tcagcatcgc tggtgttggg
     8821 aatgttggcg cagggcgtcg ccgcgttggt cgcagccttc gtgttgcggc cttggcgtcc
     8881 gggcatgcac ctgaacgtga gcacgcttcg ccctatcctg cgcttcgggc tgacttttca
     8941 ggccaaacac atcgtgggct tcgtaaacgg ggccgtcatg ccgctctacg cgggcagagc
     9001 gctcggcccc tacgcgctgg gcctcgtgac gtggtcacaa acgacggcgt tctttcccgt
     9061 caaactggtc gaaatcctgg gacgggtgaa ttttccgttg ctcagtcgac tccagcacga
     9121 cccggaggcc ctcgcgcgca cgctcgagaa gaccctacag gtttgcttga tcgtcaccat
     9181 gctgttcgtg tccctcatcc tcgggcttgg gcccagcatc gtgcagatca tttacgggga
     9241 taagtgggtg cccgcgctcc cgactctgta tgtgttcacc ttcgcgatcg cgatcgggtt
     9301 cgtgagcccc gttatgaaca gcgccttgga tgcgatcgga cagcctcgtg tcatgctgaa
     9361 gctcgctctg ggttggacgc tcctgaactg gctcgtggtg gtgaccacga tgcagttcat
     9421 gcgcagcgcg ctcgtttttg ccctcgcttg ctcggtccac gttctagtcg ggaatctcgc
     9481 cgtcatcctg gtggtcaagc agcagatccc gggcgttcgc ttatgggcgc agagccgcgc
     9541 cggcgtcggg gcagcgatcg ccggagcctg cttcggccgt tggttgctgt tgagcaggat
     9601 cgcgggaccg ttctccctga tcgcagccgt cctggtggtg gcggccgtat tcacggccgt
     9661 gctcgcagtg ctggatcggt ctgccgccat agcgctgctc gcgctagtgc agcgacggag
     9721 caagtctgct agtcgtgccg catgagcgac gccccagtcc cgaacgacta ctacgagcac
     9781 ccgcggcccg aggtggtcga ttgcgtgccg ctgagcgcac ggaatgttct cgacgtggga
     9841 tgcgcctcgg gcgcgctggg acatagcctc aagctcgcgc ggcctgaggt gcaggtgcgc
     9901 ggcgtggagc ccgtggcggc acaagccgag cgagcgcgcg cggtcttgga cgacgtttta
     9961 cacggtggag ccgatgaccc gctgccctcg cactggccgg cgccggagtg tgtgattttt
    10021 gcggatgtgc tcgagcacct agtagaccct tggagcacgc tgcgacgcta ccgatcgatc
    10081 ctgaagcccg gagggacgct cgtcgcaagc attcccaacg tggcccaccg agtggtgctg
    10141 agagggctgt tccgcatgcg ctgggattac acggattttg gaattctcga ccgaactcac
    10201 ctgcgcttct tcactcgcga taccgcggtt gagctcgtcg agtcctgtgg tttccaaata
    10261 aggagcatcg ggcgcaccac ggagggcctc gggcgagggc tattcagcaa gaaggtccgg
    10321 gatgtgttgg ccaaagagaa cggctcccgg cgcgtcttcc cgcgtccgct cgcctggttg
    10381 atcgatgcgt atactgttca gtttctgatc gtggcagagt gaccaggttc tgaccatggc
    10441 agagtaagac gtgcgcttct tctggactga cgaggatcgg ccggcgcgcc gtcgcggctc
    10501 ggcgctccgt gcggcgctcg cgtatgcggg ccatagcgtt catcgcggag tgatcggcga
    10561 cgagcctccg cctggaaccg acgtgtggat ttacgggctg ggcgtcgtcg gcgcgcccgc
    10621 gattgagccg cgcgtcgtcg gtcagttgct cgacgcccaa gcggcagtgg ttctgaccca
    10681 gctgtgtgac gccgagagca tgtgcttcga gcgcatcccc catgagctcg cgatccgggc
    10741 gcggctcttc ctccggaacc actggccaag cgactcccac tcgatcccgg ccgcttttcg
    10801 tgaccgcatc ggttgggcgc ccccgatgat caagtccatg ccggcgcggc cagggaggct
    10861 cctgcgagaa cgatcgcacg gggcgatctt ctttggtacc cggacgggtt tctccaacat
    10921 gcccgggaac aagaacgcgc gggaggaggt cgtgcgcatc ctgcgcgctt cgggtctccc
    10981 cttcttgggc gggctgctgc atcacggggc ggagtgctat cgcacggagc cttcgttgct
    11041 agtgcccagg ctgcgagagc gcacccacct caaacacctt gccaactcca agatttgcct
    11101 ggctccctgg ggcaaccatc gcctcactta ccggctcttc gagggcatgg cgatgcgttg
    11161 tctggtgctc gcgcagccca ttcgttccac ccgtttcctg gacgcagggc tcgagcccgg
    11221 cacccactac gtcgaaatcg cgccagatct ctccgacctc gcagacgcgg tccgctacta
    11281 cctgaaccat ctcgatgaag cccagtgcat cgccgatgcg ggccatgagc acttcaaaaa
    11341 gtacttcgct gctcgcggtc cgctcatctc caagtggatc ttcgattcgg tcgtcggctc
    11401 ttggggcgac ctctaccgct cggccgcgcc gcgccgcgcc cgctcggttg caatagccgc
    11461 cgccgcgcga cttcgaccct cggcattctg aacttgccgc taaactgcgc cttccaacat
    11521 gaacttcact gcaactctcg agtccgaatc acaccacgtt aggtggtgat attcagacgt
    11581 tggggttagc ggagccgcta tgcagaaacg ttgggccttt atcgctctcc tggttgccgc
    11641 tgcttgcggc ctccagttca tcggttgtag aggaaacctc aacgtctacg acgaggggtt
    11701 agccctcacc ggtgcgatgc gggtggcgcg gggggagctt ccgtataggg acttctggac
    11761 gatctactct cccgggcagt tctatctgct cgccgccttc atgagattca cgggcgaagg
    11821 gttgatcgtc ctgcgacatt tctacgccgc cattttggca gcgctcgcgg tttcgagcgc
    11881 agttctcggg ggttcccttt ctcggggatc ttcgagtcgg cggctgaccg ttacttctat
    11941 tctactcgtc atcagcgcca ctttgtgcac gagctatcgg cactactttc acgccgctcc
    12001 ggtcgccttg ttgatggcga tgctcgtgct gctgctaggt gcccaaccgt ccccccggcg
    12061 attcttcccc gcgggactac ttacgggctt ggccgttctc tttcgtcacg acatcggcgt
    12121 ctatctgttc ggttcagtcg ccagcgtgtt gctcctgtat ccgcaggatc ggacacgtcg
    12181 gcttgctccg ctgggctggt atgtcgcggg ctcgtgcgta ctccctgcat cgtgtttcgg
    12241 cgcgctggtc tcagccgggg cggggagtgc gatgtgggac caactcgttg tttttccgct
    12301 gacggtctat ccgggggtgc ggtctttgcc ctggccgctg tttcagggct cgtggtcctt
    12361 cccaagagtt gtcgagacat tagcgaccaa tgccgtcatc cttctgttcg cctatcagtt
    12421 ctggaggttg ctcggcttgt ggcgagactc ccatagggcc cattcggcac cggattcgcg
    12481 aattgtcatg atgttcgcat tcggggttgc gtgtctgctg ctcgcgaaga cacgcccaga
    12541 tttcgcgcat gttttcccat cggccatgct cgcgctctcg ctgacccccg cactgctgtc
    12601 tgaattctcc gttggatcga aggtgctcgc tttggtcgtt gctacgctgt ttgccttgac
    12661 tggcgtatcg gcgagctggc ggcagtggcg tgcgagcaat ggttattgcc tgagttccgg
    12721 tgtcggccga ggcgtatgcg gcggagatgc aacgcgagag tacgacgagg tcatcgcgcg
    12781 gctcggggtt ctcacccgac cgagagagcg gatctacgtc ggtgaggccg tccacgatcg
    12841 catgctcacc agcgatgcgg cactgtacta tctcgcgggc cgcatgcccg catgtccctg
    12901 gcacgagctg catcccggag aggcaacgaa aggcaccgta cagaccgcga ttgccgagtg
    12961 cctcgagtca acgaaagtta tcgtgctacg tcagagtaag ccgtcccgag aaccgaatct
    13021 ctcgtcgaag cccaccggag tcgatacgct agatcgtttc atcaagacgc gttacctacc
    13081 cctcgacacc ttcgagcgtt attcgatact gacgcgtgaa ctacaaagat gacgaatagc
    13141 gctgtgggtg ggctcgagag tcccgtactt ccaggcggat gctggagttg ctcgtcccga
    13201 gcgaatagat ttgcattgaa acgtttgaaa cgcgttccaa tcttccaagg tgccgcgcct
    13261 cgaacccgcc aaatctgctg cctcgttaat cgcccaggcc ctatccctgg cggtgggccc
    13321 tgcgctcgtg ttggatggcg atctgcgggt attgctcgca acggcggatg ccgcacgagt
    13381 actcgggttc gatgtgccgc tcggcgcgtc ggcgcccaag cttctttgcc gcaactcgcc
    13441 caagcgcgag gtcgcggcgg ccatgagcca aggcctcgcg atccagacca ccatcgcgca
    13501 tccggccgat gacgcaggcg aacgtcggct gaagttgagg gcgctgccgc tcggtgcgcc
    13561 gccaaacgca ggtttcctgc tgatgttgga cgaagcgccg tcgtcgagct ccgaaccggt
    13621 gttgttccaa ggcatgtgga ccgagaacgc tcgcctgaag gaggtgttcc ggatcatcga
    13681 gcgcgtggcg gtggacgacg cgaccgtttt ggtgcgcggt gagaccggca ccggtaagga
    13741 gttggtcgcg cgcgccattc acgcgctctc acctcgcgcc ggggggcctt ttcaagccat
    13801 caattgcgcg gctttgccgg caaacctgct cgagagtgag ctgttcgggc acgtgcgggg
    13861 tgcgttcact ggcgcagatc gggataccaa ggggcacctg cagctcgcgc acaaaggcac
    13921 gctgtttctg gatgaagtgg cggagatgcc gctggagctc caaccgaagc tgctgcgcgt
    13981 gctcgagacc cgcagcgtgc tcccggtggg tggtcggacc ccagtcccga tcgatgtgcg
    14041 cgtgatctcc gcgacccatc gcgcgctccg tcgcgaagtc gaactcaacc gcttccgcgc
    14101 agacttgatg tatcgactgc gtgtgattcc gattttccta ccggctttgc gtgagcgcaa
    14161 agaggatatc gcgctgctga tcgagaagct ctgcgaaagc atgaacaaag cggcgcgccg
    14221 caaggtggag cggatcgccc cgagtgcgct cgcgctgctg gagcggcatg actggccggg
    14281 gaacatcaga gagctgcgta acgtgatcgc ttatgcttac gcaatcggcg aaggtccgat
    14341 tctgcagatc aaagacttgc cgattgagct cgtggacgcg ggggggccgt cagaaggagc
    14401 gcaagcgagt gagcggctgc gcgagttggc cgctcgcgat cccgaggtgc gtcgcatcct
    14461 gcaagcgctc gagaaggcgg gtgggcgccg gcaccgggcc gccaagatcc tgggaattag
    14521 ccgcgtcacc ctttggcggc ggatgcgcga gctgggcctc gatgaggacg agccctcggc
    14581 cgagcctcga gactgaacgc ttaggttcat tgcggagcac tcggggtcat ggcggactcg
    14641 gggcgttccc cgcgcagccg gtgaacaggt tgccctccgc cgggctcttg cggcactcct
    14701 cttcatcgaa gaaccaacgg cctccttcca acccaatcga gcgcgcgtcg accgagctga
    14761 ggcgcacgtt ctgattgcac gcccagtccc gcgtatccgt gggggagctg gcggcgccgg
    14821 cctggagcgg gccgtagaag cgaatttcct ctgcgccttg ccagtagtgg aagcccagcg
    14881 ccgagcccga cgggctcgct cgccaagccg ttcccatcgg cgctcccggt gaaagcggct
    14941 cgagccgcat ggcttcgcag cgctcggtat tggccagctt gaagtagagg gttcgtcgtt
    15001 cacgcgacaa cgcggcgaag gtctcgccgg gcaaggcatc acagaagaaa ttcagcagcc
    15061 gttcgttttc gagcaccgag gtgaacgtca gctcgtcctt ttggaaggtg cccgcaaact
    15121 ccgcgtcgaa gctctccatc tgggtgacca gcttgcgcat cgagcgcagc ggcatactcg
    15181 cgccgcagcg ctcgtcgcgc agaaaaccga tcggcaatga accaaggctc atcgagcctg
    15241 ctcgctgctc cgcgatcgcg atctgcttca gattctgagt gtccaacgtt tggcaaccac
    15301 aacccggcaa cgccgcgagc aagcccttct gtaggttggg ccagcgctgc tccgcgggcg
    15361 tgctggggat cgcggccgcg gcctgctcga tggcaggaca cttggaatat tggcgatagc
    15421 cgcgcaacgc cagctcggcg cgacgtgccg gatcacgctc cctcaagagc tgaccggcaa
    15481 aagagttatc gctgccacga ctcgcggcgg cacttgcctt cggttcgcct ggcaccacct
    15541 ctggcacctt cacgagcagc tcgacagcca tacccgtggg caccgcccgc aagtagctgc
    15601 gcaccgtgcg cacgtcggtc gccgcgggtg ccacgacgta caggagagcg ttgcgagagg
    15661 tgtccttcaa cgccgctgcg agcgctgctt gggccggggc ttggagaggc cttccgtcga
    15721 gctgcgcgcc cgtctcacca atttcgagat gcacccccat ccggggcggg cggcccagcg
    15781 ccgactccgg caaatccgcg ccaatgtttg gcgtgagcgg gttgggagac agcgcgccga
    15841 tatactggat cagctcgctc ttctgcgctt cacagctcgc gcccgcctcg aagtgagccg
    15901 gttggagttc ccctgtcgcc gcggaaccct ggcagccact gaataaggcg gccatgcata
    15961 ccgcggctgc gcacaccgcg acggctggcg ccaagcctag cgccgacaag cgggacggcc
    16021 cactgaggcc gtagacttgc tcactggatg gctgcctgag gcataaacgc cggaaccggg
    16081 aacgctcgcg catgcccggg ctgtaccacg atcacgaggt acccgatcat caagctcagg
    16141 aatcagggcc caagagcagc tctgtgctac tgcccgagat tgagagcggg cggtccgaag
    16201 tctccggcgg tagtgctaag gcccgtccgc gcaattggag atatccctcg ggtcgcagca
    16261 cgtcccatcg gagaagaaca tgacaccggt ggcaccagga caagtttcac cgaccacgca
    16321 gagacaaagc gtggtgatgc cgctctcaca acaaggttcg agcgtgcgcg tcttgttctc
    16381 atcgttcggc ggatcttcac tgcctgcctg tcccgcggct ggcgctgcgg gcatcgtctg
    16441 cgaaccaccg ccttgaccat tgccaacgtt gccgctgcag tcggtgcttc cggagccctc
    16501 ggtacacgtg ctcccgttga cgtttcccgt gcccccgcta acggttgtgg tttgctcctg
    16561 agtggcactc gctccgccta aaccgccagt tgcgttactg tggccatcac tcccggggcc
    16621 gcacgctacg ccaagcgtgc agacgataga cagcaacgca gttagccaga ttcgctgcat
    16681 gtcgggattc gtctgtcgat tgcggcgctg gggattgtca ctgcgccgac agccacctgg
    16741 taccggttcc gcaggcttga ccgacgcgag ctgcgactcg ggcaaatgcc caagaacaga
    16801 cgaggaatca gcagtcgatc gccgcgcatc agacctcgca ttcgagcgat gcacgcgaac
    16861 tttcacctgc ttgacgcgca tgggggtctt gcgtgcgggg ttttccgtat gtgtgcggtt
    16921 ctccgcacac ggcagcaccg gctttcccat gttctccctc tcttgaacag acgatacccg
    16981 cgaatcgacg tgttgtccaa gaataacctt gtccacaaca tcatccacaa gtcgaaatcc
    17041 gatgtagtcg actctccccg caatcccgtg ctccgtctaa cccctcagcg cgtgtggtga
    17101 gcggtgggta tgaggctgct tctacgcaat tggctacgaa gcgccacccc ccatgatgat
    17161 tcatgagcgc acggactgcg ggatataccc gcaagacggt cccaagctcg tctcgatgtc
    17221 cgatctaaag gagcccggtc aactaacttg gatcatgcgc tacgtcggcg gctcgcgcgg
    17281 cgaccgcgcg tcaccaagag taatcccaaa ctcacaccca ggaacggtag ccaagtgggc
    17341 ttgcgggcag cgaagtgtgc tccaccgcag cctgcggcct cgtttacgaa acccgcttgg
    17401 tagagctcgg ttaccgcgga catatcgccg ctcgtcagtt cacgtttgtg cgtctcgcag
    17461 cggttcgtgc agtagtacat ggtggccgag gcgtcggtca tgtcttcgcc taagccgaag
    17521 aagtgtccga cctcgtgcgt gaccacgttt tgcacgtcgt aggattgctg ctcgcactgc
    17581 acgttccgcg cctcgctggt acaggaagcg tgtgaggagc tgatgtgcgc tgcggtcgcg
    17641 ctgtcttcgc ccccggcctg gctcccgttg cccttgccgg atgccgtgac cgcgttctcg
    17701 gactgctggt tcagaacccg atagggataa agtgaattga tcacgatatc cgcctccacg
    17761 atctcgcccg tgtcctcgtc ggagtaagtg agtgtgatgg cgagatcgtg ctcgtgcccc
    17821 gcaatcgtga tcggcgcgac cgtaaccgag ttcacgccgt ccggttttgg ctcggcgctg
    17881 actttgctgg tgcgctcgaa gtgtgggttc ggcaactgca cgccggcttt ggtccaggcc
    17941 gtgaacgctt ccttgatgct gttgcgcgcg gttggcccca acgtgtcgat cgagtcgtcg
    18001 agcgcgacga ccacatccgc gtgttgccag cgcaccgggt ggcccttggc ggattgcttg
    18061 aggctcgctg tggcaggatg ggcggagtcg ggaggaacac gactcgttcc gtgctcaccg
    18121 cgactgtgcg catatgcgaa cagaccgagg gacgtggccg cggctaccgc actcaacccc
    18181 aggtaatgcc agatgccgaa cgcttgcttg ggccgcgcgg atcgccgtgg attcgcctgc
    18241 accggcgcgc tgcgtgccgt tttgggctgg ttcgatgcgg ccagcgcatg cagggtggag
    18301 gggatgagct gcaccggcgg cggcgggcgg tggctcggcg ggcaagtggt cacggaaggc
    18361 tcggtcactg cccaaaagaa cggcgtattt cccgagaagt tgagggttcg agcgtcaggt
    18421 ttctgtcgac ttcgcggggg aagctataac tccggcgatg ttgagtgaag ccacgcgccg
    18481 cctgatccac gagcgcctca gcgccgaggt gggacgcatc gacaaggaag cgcccgcgcg
    18541 cgtagcgttg tgttatccgt caccctatac agttgcgctg agttctctcg gatttcagcg
    18601 cgtttatgcg gccatccaag ccatgcccgg cgtgatgtgc gagcgcgcct tcctgccgga
    18661 cgatgccgcg ctcccggccg cgcgggtctt ggaacggcca gtgacctacg agcaccgacg
    18721 tgagctctcg agctttccgg tgctcgcgct gagtgtggcg tacgaactcg agctcgcagg
    18781 cctcgtcaaa ttgctcgagt cttccggcat tccggctttg gcgcaggagc gctccgagcg
    18841 ccaccccttc gtcctcgcgg gcggcccgct caccttctcg aacccacagc cgttgtccgt
    18901 cttcgcggat gcgatcgtga tgggtgaggc ggaggagctc gtcgcgcccg tgctgcaagc
    18961 catcttttcc gccggttcac gcgcggaagc gtgcgaggcg ctcgccaaca tccccggtgt
    19021 gttcgtgccg agcatacatg gtgaatcgca ccccaagcca gccgcgtgcg acgatgcgct
    19081 gctcccggcc cacagcacga tccgcacccc ccacaccgaa ctctcggaca tgttcctgat
    19141 cgaggtggag cgcggttgtt cgcgaggctg cacctactgt gtcatgcgcc gcagcaccaa
    19201 tggcggcatg cgcatcgtac ccaaagaagt cgtgctcgag aaagtcccgg cggatgcgcg
    19261 ccgcgtgggg ctcgtaggcg ctgccgtcag cgatcacccc aagatcaccg aaatcgtgcg
    19321 cggcctggcc gagcgaggcg ttcaagtggg gttgtcctcg ctgcggcccg atcgcttgaa
    19381 agaggagttc atctcggccc tgaaggcggg cggatatcgc accatcacga ccgcactcga
    19441 cggctcgagc gaacgcctgc gcggcatcat cgagcgacgc ggccgcgagc caaactacgt
    19501 cgcggccgcg gagctctgcc ggaagtacgg aatggaccgc ctcaagctct acttgatggt
    19561 gggtctgcct ggggaaaccg accaagacat cgacgagtgc gtgcgcttcg tcggcgagct
    19621 ctcgcgcatc atccccatcg cgctcggcgt ggcccccttc tgcgccaaac gcaacacccc
    19681 gctcgatcgg atgccgtatg ctggcgtcaa cgtcgtgcag agtcgactcg agcggctacg
    19741 tcgcggtcta cgcggtcgcg ccgacgtacg cgccacgagc gccaagtggg cctggatcga
    19801 atacgtactc tcgcaaggcg gcaccgcgga gggcctcgcc gtttacgagg ccgtacgcgc
    19861 gggcggaggt tacgccgagt atcggcgcgc tttcgccaaa ctcgggcaca gccctgacgg
    19921 ccgcggctac gccgaagtcg tgccccccat cgcccccgag cgcgccaaga tgcgtcgcct
    19981 ctcgctcctc ggctaacttt ccatcatcga gttgggccgc ccgcaatcac gggcgcgaag
    20041 ctcgtccaag cacgttgatt ggcgcagtcc aagctctcga gagcgcccaa atcaccgcgc
    20101 aaacggcact cagcaacgcc gcatcggggc cgcgcctgcg ctacgtgcgg cgccctcgaa
    20161 gccacatgga ccgcggcact agtgacgcag aaagaacact ggtggctcag ggacacgctg
    20221 tagcgtgaaa ggtacaacct cccgggccct ttatttgatt tcggcaggct tctatcacac
    20281 atgctcagta atagaggcca agcaaagtgt tggggaaata acgaaagggg ccagttgggt
    20341 gatgattcga cggtaggaag gaacgttccc gtacgagtcg aagaactgag cgc
//
LOCUS       DASTKU010000581         5056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1004735, whole genome shotgun sequence.
ACCESSION   DASTKU010000581 DASTKU010000000
VERSION     DASTKU010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004735"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="VFQ61_20285"
     CDS             complement(<1..1492)
                     /locus_tag="VFQ61_20285"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HET9956851.1"
                     /translation="MANIRKDESRLGNEDTASRRALLERAGLLVVAGSAALLAGCEAD
                     ARGDDAAVAIDEALTGNGSVIWCDSVTEQLTLSGSNGVICVRRGWSTPGDGGGGVFVW
                     KTGTIGQSVDPLTFSGTTVTSGGGVIVVPRSGGGSWRRIFSGPLNVKWFGAKGDGSPA
                     AAAIQACIAAASALPSVIRPSTFKAGTTAGAQSTPAPNFAVYIPSGLYRLEAGLVTGS
                     NTYMYGDGPSSILFSPAVGSMPFHMVTIPTATDGIRIANIAFAGLNGINLETAKLDPS
                     LYVQSEAFQSCAIHLQIPGEGSVLGSISNVVIERCSFSNLYGFLINGLDTLGDVHLRF
                     SDNYGEYCGNGVNTCASNTIISNNILHSCEGIETSGSNILVSNNVLSGAHALIAVGGA
                     TAPPAPSNISIVGNVIDLTAANQEFAAENVAIGINGATDVSVQGNTILNPPQFGVTVL
                     GTPHIPGPGINISQRISIAGNTIRTSIDRPGVSNLIYIDGGSEDISV"
     gene            2081..3196
                     /locus_tag="VFQ61_20290"
     CDS             2081..3196
                     /locus_tag="VFQ61_20290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956852.1"
                     /translation="MVERLASNTLGIGLLVTGVLVGGCSADGSAPQPTPNLGPGAEYP
                     ADDPGTRAFLQSEPGSPSFSASVQTETETVRARRIRFQASIKEFPTPNTQPLDEATPG
                     WAAKNGVEAWFSALNFSGAYTTIGNWNTDICVLRGFRGDFDPTIPNSPTVGVGVLDAA
                     NGNIVFQASNTLAEAYAYCTPRSTFSGPNNFWPYQDVRDYRFMWDPNPLPNVIGDTAG
                     LSWNVAIPFFSDQYAFLLSGLGYNFTDAASAVSVFRGTGSLRSKSPYRIYVQATPVRL
                     RDNVGGVKVSGSNGTGDPALVTAWTVSSVGPVNNILQTGKSKNTHVCFFSSVSGNFQA
                     DTWAIFNLNANNQWQVDARGGTNGSIKLGVLCMPRAQ"
     gene            complement(3294..3617)
                     /locus_tag="VFQ61_20295"
     CDS             complement(3294..3617)
                     /locus_tag="VFQ61_20295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956853.1"
                     /translation="MSPNAEDYLALFLNYRDESGGHDFGQAYLEPEDERYRFLFEQVC
                     RLLVKSSAFNEALPQEFRRTARLYVRSDPQTLAHMRSAEMRHFMLSDLYDYVHLCSRL
                     GQAPW"
     gene            complement(3784..4320)
                     /locus_tag="VFQ61_20300"
     CDS             complement(3784..4320)
                     /locus_tag="VFQ61_20300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956854.1"
                     /translation="MPPSDQGRSPPRGELAALARKYRELSRLRLAPGPAPPAELAALS
                     REFPGSLRELETLPAEELARRARSADAALAGSAIEPVLEWVAEYHALVRLVLTVKRKL
                     AGRHELSGADAILLAREVSEQAATAVSPGLVERIASPGDGRLNDVVLTELATQLGTSP
                     ERLQHALFGEQERACSAT"
     gene            complement(4332..4868)
                     /locus_tag="VFQ61_20305"
     CDS             complement(4332..4868)
                     /locus_tag="VFQ61_20305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956855.1"
                     /translation="MNLIFSIAPSDSFDLGGVIKSAYRYLDGSVTVLQEMGCVYDSSR
                     RHGSGHDAFGTSVGFEKPFGVLFLISLKQGGTAIFRVTPRPSTPADFESAKAAAGKSG
                     ETDLGEIAVRCQTVWEVVGDVAPEFNSTLPPTRATVLGMCAVLAAVARGSILLEDGSL
                     LDAKSAWERLAELLTPAA"
BASE COUNT          931 a         1531 c         1629 g          965 t
ORIGIN      
        1 cgaccgaaat atcttcggaa ccgccgtcga tgtagatgag gttcgagacg cctgggcgat
       61 ctatcgaagt gcgaattgta ttgcccgcga tcgagatgcg ttgggagatg ttgatgccgg
      121 gccctggtat gtgaggggtc ccgagcacgg taacgccgaa ctggggtgga ttcaggattg
      181 tgttgccctg cacgctgacg tcggtagcgc cgtttattcc aatcgcgaca ttttctgctg
      241 cgaactcctg gttggcggcc gtgagatcga tgacgtttcc gactatggaa atgttcgagg
      301 gcgccggcgg cgcagtagca ccgccaaccg cgatcagcgc atgagctccc gataacacgt
      361 tgttggagac caagatgttc gagccggagg tctcgattcc ttcgcaggag tggagaatgt
      421 tgttgctgat gatcgtgttc gaggcgcacg tgttgacccc gttgccgcag tactcgccgt
      481 agttgtcaga aaatcgtaag tgcacgtctc cgagcgtgtc caggccattg atgagaaagc
      541 cgtagaggtt cgaaaacgaa cagcgctcga taacgacgtt gctgatgctg cctaggacgc
      601 tgccctcgcc agggatctgc aaatgaatgg cgcatgactg aaaggcttct gactgcacgt
      661 agaggctcgg gtcgagcttg gctgtttcga ggttgattcc gttcagcccc gcgaatgcaa
      721 tgttcgcgat gcgtatgccg tccgtcgcgg tcgggatcgt caccatgtga aatggcatgc
      781 tgccaaccgc aggcgagaat agaatgctcg aggggccgtc tccgtacatg tacgtgttcg
      841 agccggtgac tagccccgct tccaatcggt agaggcccga ggggatgtag acggcgaagt
      901 tcggtgcggg ggtactctgc gcgccagccg tcgtgccggc cttgaacgtc gagggccgga
      961 taacgctggg caacgcgctt gcggcagcga tacaagcttg aatggccgca gcggccggag
     1021 acccatcgcc cttggctccg aaccacttga cgttgagcgg tccagagaag atgcggcgcc
     1081 acgaacctcc gcccgatctt ggaaccacga tcacgccgcc cccagaggtc acggtcgtgc
     1141 cgctgaacgt cagcgggtcc acgctctgac cgatggtccc ggtcttccaa acgaagacgc
     1201 caccgccgcc atctcccggg gttgaccagc cgcggcgcac gcaaatcact ccgttcgagc
     1261 ccgaaagggt cagttgctcg gtgacgctgt cgcaccagat aacgctgccg ttgccggtca
     1321 gagcttcgtc gatggcaacc gcagcgtcat cgccccgcgc gtctgcctca cagccagcga
     1381 ggagcgccgc gcttcccgcc acgacgagca gccccgcgcg ctcgagcaga gcccggcgtg
     1441 aagcagtatc ctcgtttccc aaacgcgatt cgtctttcct gatgttggcc atggtcatcc
     1501 tcgcgtgaac gcgagccctg ggttcccccg tcgagctcgc aacagcactc tctactggag
     1561 cgccgcggtt cgagagcacg cgatgtgcca acagaacggt acgagcttaa gtgccaaaag
     1621 gcgggcgaat gggtgccgcg atacctgtca gttcaatgag cccccatgac tgtccgcgaa
     1681 cgtccgcgaa cgttcgcgtt gcgttgattg ggcgaattcg gcgtggtggg tcggggaagg
     1741 gggcgtcggc cgggttagcg tcacccaccg tgctcactct tcgtctcgac tggactttgc
     1801 tcatcgagag caatccgcct ctccgttgtg caaactcgag caatgagtcc acgtgtcgca
     1861 cgcgcggtct cgagagttgt cgcgcggtct ttcgattgct cagattgtgc agtcgtcgag
     1921 gtgggtaagt tgctctatgt gtgcaaacta tgaatctcgc cgcgaaatgt aactcgttgt
     1981 cttgacgttg gatgtgggtg cagggtaatt gagtcgctcg ctgacggcgt gagccgcagc
     2041 gccgctggag cggccgtcgc ttccgggaag gaaatcgaaa atggtggaac gtttagcgtc
     2101 caatacgctg gggattggtc tgctggtcac gggggtgctg gtcggcggtt gtagtgccga
     2161 cgggagcgcg ccgcagccga ctcccaatct agggccaggt gcggagtatc ctgccgacga
     2221 tccgggtacc cgcgcatttc tgcagagtga gcctggctca ccgtcgttca gcgcgtcggt
     2281 gcagaccgaa acagagacag tgcgcgcgcg ccgcattcga tttcaagcct ccatcaagga
     2341 gttcccgacg ccgaatacgc agccgctcga cgaggcgacg ccgggctggg ccgccaagaa
     2401 cggggtcgaa gcctggttct ctgccctgaa cttctccggt gcatatacga cgatcggaaa
     2461 ttggaacacg gacatttgtg tgttgcgcgg attccgcggt gacttcgatc ccaccatccc
     2521 aaactcgccg acggttggag tcggagtttt ggatgcggcc aacgggaaca tcgtatttca
     2581 ggcgagcaac acgctcgcag aagcgtacgc gtattgcacg ccgcgttcga ccttcagcgg
     2641 ccccaacaac ttttggccct atcaggacgt tcgcgattac cgcttcatgt gggatccgaa
     2701 cccccttccg aacgtgattg gagacacggc gggcctttct tggaacgtcg cgataccgtt
     2761 tttctctgac cagtacgcgt ttctcttgag cggcttgggc tacaacttca ccgacgcggc
     2821 ctcggccgtc tccgtgtttc gaggcactgg aagcctacgc agcaagagcc cgtaccgcat
     2881 ctacgtgcaa gccacgccgg ttcggctgag ggacaacgtg ggtggcgtca aggtgagcgg
     2941 ctccaacggc acgggggatc cggcgctggt caccgcttgg accgtcagtt ccgtcggccc
     3001 agtgaataac atcctccaga cgggcaagag caagaacacc cacgtgtgct tcttctcgtc
     3061 tgtgtcgggg aacttccagg ccgacacttg ggccatcttc aacctcaacg cgaataatca
     3121 gtggcaagtt gatgcgcgtg gcggcacgaa tggctcgatc aagctcggcg tgctgtgcat
     3181 gccccgagct cagtaacgag tgtagggcgg tgcccgtcgc gacctggcgg cgggcgtcgt
     3241 ttctaggagc gctccgtccc gggagcacgg acgcggcgtg ccgtcgccgc gctttaccag
     3301 ggagcctggc ccaaacggct gcacaggtgc acgtaatcgt agagatcgct cagcatgaag
     3361 tggcgcattt cggcgctgcg catgtgcgcc aaggtttgcg gatcacttcg tacgtagagc
     3421 cgcgcggtgc gccggaattc ctgcggcaag gcctcattga aggccgacga cttcaccagc
     3481 agccggcaga cttgctcgaa gaggaagcgg tagcgctcgt cttccggctc gagataggcc
     3541 tggccgaagt cgtgaccgcc tgactcgtcc cggtagttca aaaagagcgc gagatagtct
     3601 tctgcgttag gggacattgc cgatcgttgc ggcggtcaaa agggagcgag ttcgtgcgct
     3661 gtcggggagc aagctgcgtg ccgcccgagt tcgagcagga gctcccacgc gcggcgccct
     3721 ttcgtgtccc atttgctacc aaccagagtc tggttctcga caagtgtggg gcgtcggctc
     3781 agctcacgtc gcggagcaag cccgctcctg ttctccgaag agcgcgtgct gcaaccgctc
     3841 cggcgaggtg ccgagctgcg tcgcgagttc cgtcagaacc acgtcgttca aacgaccgtc
     3901 gcccggcgag gcgatgcgct cgactaaccc cggggacaca gccgtggccg cctgttccga
     3961 gacttcgcgc gcgagcagga tggcgtcagc gccgctgagc tcatgtcgtc cggccagctt
     4021 gcgcttcact gtcagcacga gccgaacaag agcatggtac tccgccaccc actcgagcac
     4081 cggctcgatc gctgagcccg cgagtgccgc atcggcgctg cgcgctcggc gtgcgagttc
     4141 ctccgccggt agcgtttcga gctcgcggag cgagccgggg aattctcggg agagcgcagc
     4201 gagctcggcc gggggagcgg ggccgggcgc aagtcgcagc ctcgaaagct cccgatattt
     4261 gcgcgccagc gccgcgagct cgccgcgagg tggagagcgc ccctggtccg atggcggcat
     4321 cacggcgagc actacgctgc gggggtgagg agctccgcta agcgctccca ggcactcttg
     4381 gcgtcgagca gcgagccatc ctcgagaagg atcgagcccc tcgcgaccgc ggcgaggacc
     4441 gcgcacatgc cgagcaccgt cgcccgagtg ggcggcaagg tcgaattgaa ttcgggtgcg
     4501 acgtcgccga cgacctccca aaccgtctga caacgaaccg ctatctcgcc gagatccgtt
     4561 tcgccgctct tgcccgctgc ggcctttgcg ctttcgaagt cggccggggt gctcgggcgc
     4621 ggggttacgc ggaagatcgc ggtcccacct tgcttgagcg aaatcaagaa gagcacgccg
     4681 aacggcttct cgaagccaac ggaggtgccg aaggcgtcgt gtccagagcc gtgacggcga
     4741 ctcgaatcgt agacgcaacc catctcctgc agcacggtga ctgaaccgtc gaggtagcga
     4801 taggcgctct tgataactcc accgaggtcg aaactatcgg agggtgcgat cgagaatatc
     4861 agattcatcg gtagctctct gactccttcc gtgcgcctcc gatgcaaaga gcttgcctga
     4921 aactgccggt gttcgatcgc gcgccggccc gcttgggttt ctgtagttgg agtgctcggc
     4981 cgaggcactc actacgaacg tcgaggctgc ggcgtcgaaa cgtgccggaa aaacgccagg
     5041 cccatctgcc ggagga
//
LOCUS       DASTKU010000582        10170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_781908, whole genome shotgun sequence.
ACCESSION   DASTKU010000582 DASTKU010000000
VERSION     DASTKU010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10170
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781908"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFQ61_20310"
     CDS             <1..193
                     /locus_tag="VFQ61_20310"
                     /inference="COORDINATES: protein motif:HMM:NF016557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956856.1"
                     /translation="KDLSWEPVRPELVVEVAYDHMQGTRFRHTAQFRRWRPDKPVEQC
                     TYAQLEVVPAQELSEIFGA"
     gene            complement(212..2365)
                     /locus_tag="VFQ61_20315"
     CDS             complement(212..2365)
                     /locus_tag="VFQ61_20315"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9956857.1"
                     /translation="MNSMLLLLLQIGVILVAARGVGLVFRRFQQPQVVGEMAAGILLG
                     PSLLGWLAPNVSAFLFPAKSLVHLGTLSQVGLVLFMFLVGLEFDPKLLRGRGHAAVVT
                     SHVSIVAPFFLGSVLALYLYPRLSDASVAFNGFALFMGAAMSVTAFPVLARILQERKL
                     VHTKVGAVTIACAAVDDVTAWAILAVVIAIVRANALHAPLWLTLAGSLVYMVLMLFVV
                     RPALRLLETRYHRAGRVSQDFLGLIFLLLLASAWTTEWLGIHALFGAFCLGAVMPKDR
                     GLMHEIADKLENVTVVFLLPIFFASAGLKTRIGLVTGAEMWGFFGLIMLVAVLGKFGG
                     STAAARLVGLSWRESSALGILMNTRGLMELVILTIGFDLGVISPALFAMLVMMALATT
                     IMTTPVIQWLYPLERLREAQREDSEAEDYTVLVPVSLPTSGPGLLRVARSLSALGQQP
                     RVYGLHLKRMEDTMLADIDLSTRAPRELALQPLLRSGLESGIEIRPLVFATQSPGRDI
                     ADLAYVKGADLVVMGWQKPVISNRILGGAVHDVMTRAHADVAVYVERTFNPWQRVLVP
                     YRAHIQDNAALESAARISATNDIPITVLHVLGAEGDPARSRAEILTQLVRAGANPERI
                     EVKIVRTEDALTTLVHEAKTGGYDLVVIGVTKDWGLTPAFFGSRHERLARETSANLLI
                     VRRALTRGGAADPTVTPLAAARAAQQTVPTATSLG"
     gene            complement(2412..3122)
                     /locus_tag="VFQ61_20320"
     CDS             complement(2412..3122)
                     /locus_tag="VFQ61_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956858.1"
                     /translation="MKEDEDPRSAGPGAIDGIAVNPGAAARISIAPRSTDPGSKSPKS
                     IALPRSKRSWALALLAYLLLVGAPLVALVMVVRFGANLSAPPSVFGSWTITETTVTKA
                     SSQTCVPLSPGEKLSIEQSGRYLRVGSTNGSLETGNAVLEGKQIVGQLRSTRKGCLAA
                     AYRLDAQLAALATGAVTTNNAEASASNASGANVNPESNPAQSPSGKTEGLIARLESMG
                     CNDCATLTFRAERRDVAR"
     gene            complement(3168..4637)
                     /locus_tag="VFQ61_20325"
     CDS             complement(3168..4637)
                     /locus_tag="VFQ61_20325"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HET9956859.1"
                     /translation="MAEDSDFETLKIGSHEVRITHPSKRYFDGPVVLTKLDLARYYLA
                     VAEGALCGVRDRPLVLKRFVNGAMAPPFYQKRAPEHRPDWLQTVTLSFPSGRTAEELV
                     ISDAASLLWVVNLGCIELHPHAVRAQDLDHPDELRVDLDPGPGVNFEDVRRVALEVRA
                     VLEENGLKGYPKTSGSRGIHINVRIEPRWSFTEVRRAALALSREVERRIPNLATSKWW
                     KEERHGVFLDYNQNAKDRTTCSAYSVRPLPDARVSTPLTWEEVPSCDPAEFTVLSVPR
                     RVAERGDPHADIDREFGSLHGLLELADRDEAAGLGDAPWPPHFAKGEKEGTRVAPSRA
                     RGARARSAAHAKTDATPNGTAQSETQAESSARPASETETVAVGHSRKRQKMPLIVVAN
                     SPDRDAALAGLERWKVAHPEAAQHLAIDDILIDSMRGRSTTWTRIRLNLRHVPETLRP
                     EPEKPDPDDDPTREYRRATRERNSETAPRVTKAKKKTAQ"
     gene            complement(4816..5877)
                     /locus_tag="VFQ61_20330"
     CDS             complement(4816..5877)
                     /locus_tag="VFQ61_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956860.1"
                     /translation="MQRGEQNNVRKLSTSGEEMRLEPRDGLFCMGVWGALLLGGCLVG
                     CGAEVDFGDAELSTSRQALTSVELSTDAESCQQIQYRGRTTWACRATASVAPRGSAAP
                     ARDWGICGLTHLEGNADANSVVRIGVSANGYYELLVGVGSRDRSHLSARMSCVRMTAF
                     AGLPSRLHARATREHAVGSHDAPDPGEKAAIRRVELHDGGPRDLCLWHGFDGNINAVP
                     KDDLDSFLFSGTRFDQAHGWSVDAAAFGRLSHQFSTFSHCESYLPGHGWAQSDSSQSW
                     DSAGADGLFRQYQYGASGPLADAASHFCWLTGVSTYGPTMSASLNIEVPRGASAPQWQ
                     YWTDTLSTMVGIQCVQYQQ"
     gene            7344..9425
                     /locus_tag="VFQ61_20335"
     CDS             7344..9425
                     /locus_tag="VFQ61_20335"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET9956861.1"
                     /translation="MRTSLTTVIPLILAAVAGACSSESSPSSGQGGRVTSSGGGGAGG
                     TGGARSGGAGGASGGVSGGLSTGGVHTGGAVGVTGGSATAGSAGSGGSVAGAGGSGAG
                     AGGVVAGAGGVAQGGAGAGAGGTAGASTGAGGSLGGSAAGATDLKCNDPIYGSVAIPA
                     TAVISDFESSSLKQYEHDGRGVNAEPWYAYAVDDVNDAHGEVTTPSVVNPKNAANNFK
                     VETTEHGPCSAKGALHVSSPGKAGSGSYAGFGIDFMARTAARKKLSYDASKYTGVGFW
                     AKCKSDLQFAYLKVPDAKQDADMDPAPCTYSEGTCNQYGIKNAVLTKEWTYYKIYFSE
                     ALQDPNGKNFGTGVDKSKLTAFQIHVNPLSPRNGSPLANPFDCYIDDVHFLTEAAPAV
                     PAATVKYTVSGNQILRNGSPYKIRGLVRPSMEWDYSGFGVTREDMQRMKTKWHANAVR
                     LAVKDTLWKGASTGSATGDGGAYQRNVKRVINWILQQGMDVILDLHYVAGMPTAEHAT
                     FWDSISKDAFFKDGRIIFELYNEPTGDFTQLRSWMDSTITKIRANGAENLLLVGGVDY
                     SYDISGYEKNPITGKGAVAYVTHPYIFKSAPGDAVAFLTPAKTLPVVATEFGDANVEG
                     FHTIPATQCVASIYSDYISKFEGAGMSWTSWAWIVDEWGCGFPQIISDYSGTPNSIGT
                     PVQQQLLKLNP"
     gene            complement(9499..9816)
                     /locus_tag="VFQ61_20340"
     CDS             complement(9499..9816)
                     /locus_tag="VFQ61_20340"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9956862.1"
                     /translation="MDAEPTREEVDRSTGPWVLEFGAAWCPICQGTRPLIDRALADHP
                     GIRYARIEDGKGKPLGRSFRVKLWPTLVFVRDGQELSRLVRPTSDSELRLAFENLSAT
                     PTP"
     gene            complement(9849..>10170)
                     /locus_tag="VFQ61_20345"
     CDS             complement(9849..>10170)
                     /locus_tag="VFQ61_20345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956863.1"
                     /translation="FRSASRGYNGEQSCFSAAVDVRVDAFEPWLTAQLVTWHGAQAAP
                     LEREAPHDSSPLQAETPSKGAPSCQSAGSVGSSSPTWLSAAIFALGWAYRRAARDPRR
                     GTPG"
BASE COUNT         1962 a         3213 c         3130 g         1865 t
ORIGIN      
        1 taaagatctg tcgtgggagc cggtacgtcc agagttggtc gtggaggtcg cctacgacca
       61 catgcagggc actcgattcc ggcacaccgc gcagtttcgc cggtggcgcc cagacaagcc
      121 cgtggagcaa tgtacgtatg cgcaactgga agtcgttcca gcgcaggagc tgagcgagat
      181 attcggcgct tgaggctcca accctctccg ttcagcccag tgacgtggcg gtgggcacgg
      241 tttgctgggc tgcgcgggca gcggccaacg gcgtgacggt cggatctgcg gctcctccgc
      301 gcgtgagcgc tcgccgtaca atcaacagat tcgcgctcgt ttcccgggcc agccgctcat
      361 gccgcgaacc aaagaaggca ggggtcaacc cccaatcctt ggtgacgcca atcaccacca
      421 agtcgtagcc gccagttttc gcttcgtgca cgagcgtcgt gagcgcgtct tcggtacgca
      481 cgatcttgac ctcgatacgt tccggattcg cgcccgcgcg cacgagctgc gtcagaattt
      541 cggcgcggct acgcgccgga tctccctccg cgcccagcac atgcaagacg gtgatgggga
      601 tatcgttggt cgcgctgatg cgagccgcac tctcgagcgc ggcgttgtcc tgaatgtggg
      661 ctcgataggg caccagcacg cgttgccagg gattgaaggt ccgctccacg tacaccgcaa
      721 cgtccgcgtg agctcgcgtc atcacgtcgt ggacggcgcc gcccaagatg cggttcgaga
      781 tcaccggttt ttgccagccc atcaccacca aatcagcccc cttcacgtac gccaagtcag
      841 cgatatcccg ccccgggctt tgggttgcga acaccagggg tcgaatctcg attccggact
      901 ccaaaccact ccgcaatagc ggctggagcg cgagctctcg aggggcgcgc gtggagaggt
      961 cgatgtcggc caacatcgtg tcttccatgc gtttgagatg caacccgtag acgcgcggtt
     1021 gctgaccgag cgctgacaac gagcgcgcca cccgcaatag ccccgggccg gaggtgggca
     1081 gactcaccgg caccagcacg gtgtaatcct ccgcctcgga gtcctcgcgt tgcgcctccc
     1141 gtaggcgctc cagcgggtac agccactgaa ttaccggagt cgtcatgatc gtggtcgcga
     1201 gcgccatcat caccaacatc gcgaacagcg cgggcgagat gactcccaag tcgaacccaa
     1261 tggtcaagat cacgagctcc atcagcccgc gcgtgttcat cagaatgccg agcgcgctgg
     1321 actctcgcca cgacagacca accaggcgcg ccgccgcggt cgagccgccg aacttgccga
     1381 gcacggcgac caacatgatc aggccaaaga agccccacat ctccgcgcct gtcacgagcc
     1441 cgatacgtgt tttcaggccc gcgctcgcga agaagatcgg cagcaaaaag acgaccgtaa
     1501 cgttctcgag tttgtccgcg atctcatgca tgagtccgcg atctttcggc atgaccgcgc
     1561 ccaagcaaaa cgcaccgaac aaagcgtgaa tcccgagcca ctccgtggtc caagcggaag
     1621 ccaacagcag caggaagatc aatcccagaa agtcttgcga gacgcgcccg gctcgatggt
     1681 aacgcgtttc caacagacgc agcgccggtc gcaccacgaa cagcatcaac accatgtaca
     1741 cgagcgagcc cgccagcgtg agccacaggg gcgcgtgcag cgcgttggcc cgcacaatcg
     1801 caatcaccac ggcgaggatc gcccaagcgg tcacgtcatc cacggcggcg caggcgatag
     1861 tgaccgcacc caccttggta tgaaccagct tccgttcttg cagaatccgc gcgagcaccg
     1921 gaaatgcggt tacgctcatg gctgcgccca tgaacagcgc aaaaccattg aaggctaccg
     1981 acgcgtcgga caaacgcgga tacagataga gagccagcac cgaacctagg aagaacggtg
     2041 ccacgatgct cacgtgactc gtgactacgg cggcgtgccc acgtccgcgt agcaacttgg
     2101 gatcgaactc gagcccgacc aagaacatga agagtaccag ccccacctgg ctcaaagttc
     2161 cgaggtgaac gaggctcttg gcggggaaga ggaaggccga gacattcggg gcgagccagc
     2221 caagcaagga cggcccgagc aaaatcccgg ccgccatttc accaaccacc tgcggctgtt
     2281 gaaagcgccg gaagaccaga ccgacgcccc gtgcagcgac caagatgacc ccgatttgca
     2341 gcagtaggag tagcattgaa ttcatcgtcg gatcctcgcc tgccggcttt gaaccgcgag
     2401 attcggagca ctcatcgcgc gacgtccctg cgctcggcgc ggaaagtcaa ggtcgcgcag
     2461 tcattgcaac ccatggattc caagcgagcg attaggcctt cggttttgcc actcggcgac
     2521 tgagcggggt ttgactcggg attgacgttg gctccactcg cgttgcttgc gctcgcttcg
     2581 gcgttgttcg tcgtgacggc ccccgtggca agggcggcta gctgcgcgtc gagtcggtat
     2641 gcagccgcca agcaaccctt gcgagtactc cgcagctggc cgacgatttg tttgccctcg
     2701 agcacagcgt ttcccgtctc tagcgaacca ttcgtggaac cgacccgcag gtagcgtccg
     2761 gactgttcga tcgagagctt ctctcctggg gataagggaa cgcaggtctg cgaagaagcc
     2821 ttggtaacgg tggtttcagt gattgtccaa gagccgaata cgctgggagg agcggacaaa
     2881 tttgccccaa accggaccac cattaccaaa gcgacgaggg gtgctcccac gagcagcaga
     2941 tatgcgagca gtgcgagcgc ccaactacgc ttgctgcgcg gcagggcaat gctcttggga
     3001 gatttcgagc ctggatccgt ggagcgtggt gcaatcgaga ttctggccgc cgcgcctgga
     3061 ttgacagcga tcccatcgat ggccccaggt ccggcggagc gcggatcctc gtcctccttc
     3121 atcgcctata agtagccggt tacggctgtg ggagcaagcc gcgcgaccta ctgagcagtc
     3181 ttctttttcg ccttcgtcac acgaggtgct gtttcggaat ttcgttctcg agtggctcgc
     3241 ctgtactccc gcgtgggatc atcgtccgga tctggcttct cgggttcggg gcggagcgtt
     3301 tcaggtacgt ggcgcaaatt tagtcgaatg cgagtccatg tcgtcgatct accgcgcatc
     3361 gagtctatca atatgtcgtc aatggccagg tgttgcgcgg cctctgggtg cgccaccttc
     3421 cagcgctcga gtcctgccag ggcggcgtct ctgtccgggg agttagcgac cacgatcaga
     3481 ggcatttttt gccgctttcg cgagtgaccc acggcgaccg tctccgtctc gctcgcgggc
     3541 ctcgcgctcg actcggcttg ggtttcggat tgagccgtcc cgttcggggt cgcgtctgtc
     3601 ttcgcgtgcg cagcgctgcg tgctcgcgcg cctctcgcgc gggaaggtgc cacgcgcgtc
     3661 ccttccttct cgcctttggc gaagtgaggt ggccatggcg cgtccccgag ccccgcggct
     3721 tcgtcgcggt ctgcgagttc caacaatccg tgtaacgaac cgaactctcg atcgatatcc
     3781 gcgtggggat cgccgcgctc cgccacccgg cggggaacgc tgagtaccgt gaactcggca
     3841 ggatcacaac tcggcacctc ctcccacgtc agcggcgtcg agacgcgcgc gtcgggcaag
     3901 ggacgcaccg agtaggccga gcacgtggtg cgatctttgg cattctggtt gtagtccagg
     3961 aacaccccgt gccgctcttc cttccaccac ttcgacgtcg ccaagttcgg gatgcgccgc
     4021 tccacttcac gcgaaagcgc caatgccgcg cgcctcacct cggtgaagga ccaacgcggc
     4081 tcgatacgaa cgttgatgtg aataccgcgg gagccgctgg tcttggggta acccttcagc
     4141 ccgttctcct cgagcaccgc acgcacctcc agagccacgc gccgcacgtc ctcgaagttg
     4201 acacccggac ccggatctag atccacgcgc agctcgtccg ggtgatccag gtcctgcgcg
     4261 cgcacggcgt gtggatgtag ctcgatgcag ccgagattga cgacccagag caggctagcc
     4321 gcgtccgata tcaccagctc ctcggcagtt cgccccgacg ggaacgaaag ggtgaccgtc
     4381 tgcaaccagt cgggtcggtg ttcgggggcc cgcttctgat agaacggcgg cgccatggcg
     4441 ccgttcacga aacgctttag gaccagcggg cgatcacgaa cgccacatag ggcgccctcg
     4501 gccacggcca agtagtatcg agccaagtcc agcttggtga gcactactgg cccatcgaag
     4561 tagcgtttcg acggatgggt gatccgtacc tcgtgcgacc cgatcttgag cgtttcgaaa
     4621 tctgaatcct cggccacgcc agtagcttag agcccatccc agtcggagct agtcgccgac
     4681 cgtcgatcgc cctcgtcccg ttccaaaggg ccgtgctgtg gcccggctgt cgcgcgggac
     4741 gggtgccaca atcgagatgg aagtccgctc accgggacgc tcttcagaat cagctacggt
     4801 ccccgggctt cgtacctatt gctggtactg gacgcactgg atcccgacca tggtcgagag
     4861 cgtgtcggtc cagtactgcc attggggagc ggatgctcct cgcggcactt cgatattcaa
     4921 gctggcagac atggtggggc cgtacgtgct cacgcccgtg agccaacaga aatgggaagc
     4981 ggcatccgcg agcggaccgc tcgcgccata ttggtactgc ctaaaaagcc catccgcgcc
     5041 cgccgagtcc cagctttgac tggaatcgga ctgagcccag ccatgcccgg gcaagtagct
     5101 ctcgcagtga ctgaacgtcg agaactgatg tgagagacgg ccaaaagctg cagcatccac
     5161 gctccagccg tgggcctggt cgaagcgtgt gcccgagaac aagaacgagt ccagatcgtc
     5221 tttgggaact gcgttgatgt tcccgtcgaa gccgtgccac aagcacaaat cccgaggccc
     5281 accatcgtgc agctctactc tccgaatcgc ggctttctca ccaggatccg gcgcgtcgtg
     5341 acttcccacg gcgtgctctc gcgtcgcacg agcgtgtagc cgggagggga gccccgcgaa
     5401 cgcggtcatt cgcacgcagc tcatccttgc ggagaggtgg ctgcgatctc tcgatcccac
     5461 gcccaccaat agctcgtagt agccattcgc gctgacgccg atacggacaa cgctgttcgc
     5521 atccgcattg ccctctaagt gcgtcaggcc acaaatgccc caatctctcg ctggcgcggc
     5581 ggagccgcgg ggcgcgaccg acgccgtcgc gcgacaggcc caagtggtgc ggcctcggta
     5641 ttgaatctgt tgacaactct ccgcgtcagt cgagagctct acgctcgtga gggcttgcct
     5701 cgaagtggac aactctgcat ccccgaaatc gacttcggct ccacagccca cgaggcaacc
     5761 cccgagcagc aaggcacccc aaacgcccat gcaaaatagc ccgtctctcg gctccaagcg
     5821 catttcctcc ccgcttgtcg agagcttccg aacgttgttt tgctcgcctc tttgcatgag
     5881 atcgccttct cgaacgccgg gtagagcggt tagcaagagc caggccactc tctcggaccg
     5941 tgaatcgcgc atgttttcca gccaaatcag agccgcggta ctgaacagca gggcttcaca
     6001 acagacttga gagcgtccac tgacctggtt ttgcaaggcc gccccgctcg tgtgtcgttg
     6061 cctcactcta tcttctaccg atctgttccc ctctatcaat cggttccccc tcatcaatcc
     6121 atccccgcca tcaatccaat cctcctctat caggctatcc ccttctcgaa tcgtttcact
     6181 accagccggc aattctgggt ctcgcgctcg cctcgatctg ccgactccgt ccgccatccg
     6241 cgcgaagcca aggcgaatcg agcgctcagg catcccgcgc gcgggggtga gctgcatgac
     6301 cctagagatg acctcatcac gtctgggtga ttgagggcaa tggcaaacgt ttagatgcgc
     6361 cacttcggtg ttccgtgcaa ctcaattgcg aaagatcgag ttgcgccgcc actgcagctc
     6421 ccagtacgag tgacgctact gactcgccac gaccgcgcag ccgcgggcta tgtaacgcgt
     6481 tgtggccagc ctcgaactcc atcggcccga gagcgatgta gttcgccgtg gcaaagttct
     6541 gacgcgggct ccccacccct gctgggtgct ggattcgcat gcatgcgagc ccgcaactat
     6601 gccgagaccg gccgcccgcg agcggaacga gtcgcaacca ataccgtcgc gagctcgcgt
     6661 tcgcttagca cgtggaaaat gggaggaagc ccccctccgt tcatcaaggc gttgcctgtc
     6721 gttgccgccg tggacagcca aagtatccga tgcggccaac aacatcgccg accccgtgat
     6781 tccgtgtgat cggcagttgt cgccgcaggt ttgctcaggt cgctcttccg aattcgagcg
     6841 caatcgagtt cgcaaggcac catgagcgcg gtaggtcggt gactgcgttt tgcaaagctg
     6901 cggtttgcaa aacgactctg gttttctgca gaatgcgtcg ctctcgccta cgtcggagcg
     6961 gcccgcgccc gccttacgga gagcctgatg ggcttatcgc ggcttggatg cggggtgaat
     7021 gattaacggc acatcgaggc ctctttcccg ggcgtgtccc ggcaaaaatc agccttctgc
     7081 gcggaaggag tggcgggcaa gcattaaacg accatttctc gtggcgcgtc ggaactcgat
     7141 gtggcaggtc tcgagcgctc ccttcctttg cggagcgccg tccatggttg ttaaatcacc
     7201 ccgcagggta acgccggcag tgtgagccaa gtggccatgc tcgcggggca agcgctcgcg
     7261 ggctcgcggg gcaagcgctc gcgcggtaag agacggctag ggtaacgaaa gacggctcgg
     7321 aaacaacagg gtaggttcaa gacatgcgaa ctagcttaac aaccgtaata cctttgattt
     7381 tggccgcggt cgccggggct tgctcctccg aaagcagccc atcgtcaggt cagggtggcc
     7441 gagtgacgtc tagcggcgga ggcggcgcgg gcggaaccgg cggcgctcga agcggcggtg
     7501 cgggcggcgc gagtggcgga gtgagcggcg ggctctccac gggaggggtg cacaccgggg
     7561 gagcggttgg agtgaccggc ggtagtgcca ccgccggcag cgcgggctcc ggtgggtcgg
     7621 tcgcgggcgc gggcggctcg ggtgcaggcg caggcggagt cgtggcgggc gcgggcggcg
     7681 tggcgcaggg cggagcgggt gctggtgccg gcggcaccgc gggcgcttcc acgggtgcag
     7741 gtgggtccct cggcggtagc gcggccggtg ccaccgatct caagtgcaac gatccgatct
     7801 acggaagtgt cgcaatcccg gccaccgccg tgatctcgga tttcgagagc agctcactca
     7861 agcagtacga gcatgacggt cgcggcgtca acgccgagcc ctggtacgca tacgcggtgg
     7921 acgacgtgaa tgacgcccac ggtgaggtga ctactcccag cgtcgtaaat cccaagaacg
     7981 ctgccaacaa cttcaaggta gagacgacgg agcatgggcc gtgcagcgcc aagggcgcct
     8041 tgcacgtgtc atcaccgggt aaggcgggct cgggttcgta tgccggtttt ggtatcgatt
     8101 tcatggcccg caccgcagcc cgcaagaagc tgagttacga tgcctccaag tacaccgggg
     8161 tgggcttctg ggcgaagtgc aagtcagact tgcagtttgc ctatctgaag gtgcctgacg
     8221 ccaaacaaga tgccgatatg gatccggcgc cctgcaccta ctccgaggga acctgcaatc
     8281 aatacggtat caagaatgcg gttctcacca aagaatggac ttactacaag atctacttct
     8341 ccgaggcttt gcaggatccc aacggtaaga acttcggcac cggggtcgac aagtcgaagc
     8401 tcacggcctt ccaaattcac gtcaacccgt tgagcccgcg caacggatcg ccgctcgcga
     8461 atccgttcga ttgctatatc gatgacgttc acttcttgac cgaagccgct ccagcggtgc
     8521 ccgctgccac cgtaaagtat acggtgagcg gcaatcagat cctgcgcaac ggatcgcctt
     8581 acaagatccg cggcctggtg cgcccctcga tggagtggga ctactcgggc ttcggcgtga
     8641 cgcgcgaaga catgcagagg atgaagacga agtggcacgc caatgccgtt cgtctcgcgg
     8701 tcaaagatac cttgtggaag ggcgcctcga ccggatcggc gaccggcgat ggcggcgcgt
     8761 atcaacgcaa cgtcaaacgg gtcatcaact ggatcctgca gcagggtatg gatgtgattt
     8821 tggacctgca ttacgtcgca ggtatgccca ccgcggagca cgcgacgttc tgggattcca
     8881 tctcgaagga cgcgttcttc aaggatggtc ggatcatctt cgagctctac aacgagccga
     8941 cgggcgactt cacccaactc cgatcttgga tggatagcac gatcaccaaa atccgggcga
     9001 acggagccga gaacctgctt ctcgtgggtg gcgtggacta ctcctacgat atttcgggct
     9061 acgagaagaa cccgattacg gggaagggcg cggtagcgta cgtcacccac ccgtatatct
     9121 tcaagagcgc tccgggagac gcggtggcgt tcttgacgcc ggccaagacc ctgccggtgg
     9181 ttgcaaccga gtttggcgac gcgaatgtcg aggggttcca caccatcccc gcgacacaat
     9241 gcgtggcgag catctactcg gactacatta gcaagttcga gggggccggg atgagctgga
     9301 cctcttgggc ctggattgtg gacgagtggg gctgcggatt cccgcagatc atctcggact
     9361 acagcggaac gccgaactcg attgggacgc ccgttcagca gcagctcttg aagttgaatc
     9421 cctgagtctc tcgctccgca gcacagcgca aggcttcgac gtttgcctca tgaggctgcg
     9481 tcgaagcctt gatcgcactc acggtgttgg tgtggcactc agattctcga aggcgagtct
     9541 gagttcggag tccgacgtgg ggcgaacgag ccgactcagc tcctgcccgt ccctcacgaa
     9601 aactagagtc ggccagagct tgacgcgaaa cgagcgcccc aggggcttgc cctttccgtc
     9661 ctcgatgcgc gcataacgaa tcccgggatg gtcggcgagt gcgcggtcga tgagcgggcg
     9721 agtgccttgg caaatcgggc accatgccgc accgaactcg agtacccaag ggcccgtgga
     9781 tcgatcgact tcttcgcgtg ttggttcggc atccatgaat cacagcatat ccgagctggg
     9841 aaccggagct agccgggggt gccccggcgc gggtcgcgag cagcgcgtcg gtaggcccag
     9901 ccgagtgcga atatcgcggc agatagccac gtgggggagc tcgagccaac cgaacccgca
     9961 gactggcagc tcggggctcc cttgcttggt gtctctgcct gcagcgggct agaatcgtgc
    10021 ggtgcttctc gttccagcgg agcggcctga gctccatgcc aggttacgag ttgagccgtg
    10081 agccacggtt cgaatgcgtc tacacgcaca tccacggctg cggagaaaca actctgctcg
    10141 ccgttgtagc cgcggctcgc agatcggaag
//
LOCUS       DASTKU010000583         5607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1229513, whole genome shotgun sequence.
ACCESSION   DASTKU010000583 DASTKU010000000
VERSION     DASTKU010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229513"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFQ61_20350"
     CDS             <1..209
                     /locus_tag="VFQ61_20350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956864.1"
                     /translation="AGEGDECSRDEACAYGLICLASQVGAPRRCTADVTPIATPVDPN
                     LLPTLQGGSAPASGGSTSGGGAGT"
     gene            complement(250..1272)
                     /locus_tag="VFQ61_20355"
     CDS             complement(250..1272)
                     /locus_tag="VFQ61_20355"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET9956865.1"
                     /translation="MNQRSIEVKVGALILVALGLLGAFVVIMGGLSFEPSFTVYVGFQ
                     NPGGLQAGAPVRISGVKVGRVSSIEFRGGKLDPSSKKPESIIRAVAKIERRYQEAIRE
                     NSRWFVTSQGVLGEMFLAVEPGTYDRPILADGANVQGISPPRLDLLLSESYELLHRAY
                     LGITNNEQQLAETFEGLHRTLKASGDLLERNGPKLDRTLDNVEQLSSSARDAVSAARE
                     RYVDNPQITRILNDVERSSGALARNLEPTLTDGRDALQSGKKFLTWLSQDTQLQRLDA
                     TTRDLAAAAQHAKQVGAEAEALVRHVQQGKGTLGALAKDEAIYDDIQELLRDLKHNPW
                     KFFWRQ"
     gene            complement(1283..2023)
                     /locus_tag="VFQ61_20360"
     CDS             complement(1283..2023)
                     /locus_tag="VFQ61_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9956866.1"
                     /translation="MISFRGVSKAFGAKQILKDVSFDVAPSQVFFIIGASGVGKSVLI
                     KHLIGLIRPDAGEIWLDHEEVSQLSERDFYRVRRKCAMVFQHATLFDSMNCLENVALP
                     LIKHDGLRATEAFDQARDLLRAVHMQEFAERFPSELGDGLRKRVAIARALAVRPRYVL
                     FDEPTTGLDPVSARRVDRLIRELCDERGVTSVVVSHDLTSIFEIADRIVMLYKGQVRM
                     LGSPEEFRRSDDAIVRQFVSGSAQGPME"
     gene            2247..2987
                     /locus_tag="VFQ61_20365"
     CDS             2247..2987
                     /locus_tag="VFQ61_20365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956867.1"
                     /translation="MTCAAPHGAKYAWASGSGLLRSYRGQIQQLYRKANGVVLAIALP
                     IAVALPLSVSQGCASSEPPRREREALRQDLCPADSVRQFHCDDLLPRPKSQEGAPAPY
                     ELCPAGIQVPLTDGYPANQSVAEFDVPYTEWARRRVSPGHACCYSWCAALPPADPEDV
                     PPEAGCAEPSVTRESYCLPELERGSSIAASEPFERCPMVIHPPAGQFFSVPKAAPLDP
                     QATAGKRGAGEEVCCYAWCSRMPAGILK"
     gene            complement(3027..3767)
                     /locus_tag="VFQ61_20370"
     CDS             complement(3027..3767)
                     /locus_tag="VFQ61_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9956868.1"
                     /translation="MDFFKALYGTFATTLYYVFRGRSERGSVLRQMYEIGNRSLVFVC
                     VVLGFIGTILVYQGGLQTQRVVPDLSGLGPTFLELLVKDLAATICALMLATRVGAGIA
                     AEIGSMVVTEQLDALRMCAADPIDYLVKPRFIACLCMTLVLVVIAGSVAFGTGLGAAY
                     VFFEIRPATFASLSLVDVSDVVVGIAKCVAYGAAIPIISAHAGLMTSGGSEGVGLATT
                     RAVVNSSLAVIVLDFLISGAGYVAFGAG"
     gene            complement(3809..4609)
                     /locus_tag="VFQ61_20375"
     CDS             complement(3809..4609)
                     /locus_tag="VFQ61_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9956869.1"
                     /translation="MAELVPSEAPEGALTRAALALSEQARLLGSMALLFASICGAALR
                     RELDGLEFIRNLHRMGVKSLPIVATTALFTGGIMVIQAGPLVERYGAQGLLGWGAGFG
                     TLREIGPLLTALMISGRVGANNTAELGTMAVTEQLDALRALAIDPQGYLVAPRFFGIV
                     LTLLLSTVFSDALALFGASQAGWALLGVEPTVFFQGLTSELLGFGDVLHGLIKSVLFG
                     GIIGLCSCHFGISTAGGAPGVGRAVNATVVTSAVGIFACDYLSSFALG"
     gene            4730..>5607
                     /locus_tag="VFQ61_20380"
     CDS             4730..>5607
                     /locus_tag="VFQ61_20380"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9956870.1"
                     /translation="MTQDLRIGVIGLGYAGTRHARALQDGRIPGARLHAVCDQDPGRA
                     AAFPDSVHARLDDIFAEPALDAVVIATPHRSHVELCENALREGLSVLVEKPFGVHAAE
                     CRRALAAHSDAIRSAALHSATKRTETAPQEQVSARIQRSVMPLLLGVVHDYRVEPKFI
                     WLKELIQRGELGRVVRFTWQATNFFRTEAYFQTSPWRGRFLTEGGGILVNQAPHLLDA
                     VAWLFGVPRRLFASCGFGRYHQIEVEDDVNALLEYADGTRGNLQLSTGESPGTQRLDV
                     SFDGGRVVVEGNEAVI"
BASE COUNT         1045 a         1755 c         1794 g         1013 t
ORIGIN      
        1 ccgcaggtga aggcgacgag tgttcgcgag acgaggcctg cgcctatggc ttgatctgtc
       61 ttgcgtccca ggtcggcgcg ccacggcgtt gcacagcgga cgtgaccccg atcgccacgc
      121 ccgtggatcc gaatcttttg ccaactcttc agggcggaag cgcgcctgct tcgggcggaa
      181 gcacgagtgg cggcggcgca ggtacgtaaa cgggaaagaa cgagggttcg gcgcgaagaa
      241 agcctgcgct cactgccgcc agaagaactt ccagggattg tgtttgagat cccggagcaa
      301 ctcctggatg tcgtcgtaga ttgcttcgtc cttggcgaga gcgcctaggg tgcccttacc
      361 ctgctgaacg tggcggacca gggcttccgc ttcggcgccg acttgctttg catgttgcgc
      421 ggcggccgcc aagtcgcgcg tcgtggcgtc cagccgttgc agctgcgtat cctgactcag
      481 ccacgtcagg aatttctttc cactctggag cgcatcccga ccgtccgtga gcgtgggttc
      541 caagtttctc gccaacgccc cggacgagcg ctccacgtcg ttcaaaatgc gggtgatctg
      601 agggttgtcc acgtaacgct cgcgggccgc gctcacggcg tctcgcgcgc tgctcgagag
      661 ttgttcgacg ttgtccagcg ttcgatccaa cttggggccg ttgcgctcga gcaagtcacc
      721 ggaagccttg agcgtccggt gcagcccctc gaaggtctcc gcgagctgct gctcgttgtt
      781 ggtaatgccg aggtaagcgc gatgcagaag ctcgtagctc tcgctgagca gcagatctag
      841 gcgcggcggg ctgatgccct gcacgttggc cccatccgcc aatatcggcc gatcgtaagt
      901 tcccggctcc actgccaaga acatctctcc gagcacaccc tgactcgtca cgaaccagcg
      961 cgaattctcg cggatggcct cttgatagcg ccgctctatt ttcgccaccg cacggatgat
     1021 cgactccggt ttcttgctgc tcgggtcgag cttgcccccg cgaaactcaa tcgagctcac
     1081 gcgcccgact ttgactccgg agatccgcac gggcgcgccg gcctgcaacc ctccggggtt
     1141 ttggaagccg acgtagaccg tgaagctggg ttcgaaactg agcccaccca tgatcacgac
     1201 gaacgccccc agtagaccca gagcgaccag gatcagggcc ccgactttga cctcgattga
     1261 gcgctggttc atgactattt catcactcca tcggaccttg cgccgagccg ctgacgaact
     1321 ggcgcacgat ggcgtcgtcg ctgcgacgga actcctcggg gctgccgagc atacgaacct
     1381 gccccttgta gagcatcacg atccggtcgg ctatttcgaa gatcgacgtc aaatcgtggg
     1441 acactaccac gctcgttacc ccacgttcgt cgcatagctc gcgtatcaaa cggtcgaccc
     1501 ggcgtgcgct caccggatcc aggcccgtcg tgggttcgtc gaatagaacg tagcggggac
     1561 gcaccgcgag ggcacgggca atcgcgacac gcttgcggag cccgtccccc aactcggagg
     1621 ggaagcgctc cgcgaactcc tgcatgtgca ccgcccggag caggtctcga gcttggtcga
     1681 aggcttcggt ggcccggagt ccgtcgtgtt tgatgagggg cagcgcgacg ttctcgaggc
     1741 aattcatcga gtcgaaaagt gtcgcgtgtt gaaacaccat cgcgcatttg cggcgtactc
     1801 ggtagaagtc gcgttccgag agctgactca cttcctcgtg atccagccag atttctccag
     1861 catccgggcg aatcagaccg atcaggtgct tgatgaggac gctcttgccc acgccagaag
     1921 caccgatgat gaagaatacc tgcgagggcg ccacgtcgaa gctgacgtcc ttgagaattt
     1981 gcttggctcc gaaggccttc gagacgccgc gaaaggagat cacgctttga caatccgaac
     2041 ccgtccggta tacccaaaaa aaccggcgcg ggcttgataa acgtgggtgt ttggaacgac
     2101 gtgggtttct cgatcttggg cttgttggat tcgctctcgc tttcggaagt gggctcgttg
     2161 cggcacgacg tgcgagcaag ggtgggacga gcgtgggcgc ggtctgaagt ccaagtcgcg
     2221 gtctaccggg aaagggaggc tgtgagttga cctgcgcagc cccgcatggc gcaaagtatg
     2281 cctgggcgtc cggctccggc ctacttcgct cgtaccgtgg tcagatacag cagctctacc
     2341 ggaaggcgaa cggggtcgtt ctcgcgattg ccttgcccat cgcggtcgcc ctgccactct
     2401 cggtctcaca aggctgcgcc tcttcggagc caccgcgccg cgagcgggag gcgctgcgac
     2461 aggacctttg cccggcagat tcggtgcggc aattccattg cgatgatctt ttgccgaggc
     2521 cgaagtcaca agagggagct ccggcgccgt acgagctttg tccggcgggc attcaagtgc
     2581 ctctgacgga tggctacccg gcaaaccaaa gcgtcgccga gttcgacgtt ccctatacgg
     2641 agtgggcgcg gcgtcgcgtt tcccccgggc atgcgtgctg ttacagctgg tgcgccgcat
     2701 tgccacccgc cgatccagaa gacgtgccgc ccgaggctgg ctgcgcggag ccttcggtaa
     2761 cgcgcgaaag ctactgcttg cctgagttgg agcgaggttc ctcgatcgca gcctccgagc
     2821 cgttcgagcg ctgtccgatg gtcatccacc ccccggctgg gcagttcttt tcggtgccca
     2881 aggcggctcc gctcgacccc caagcgacgg cggggaagcg gggcgcgggg gaagaggtgt
     2941 gttgttacgc atggtgttcg agaatgcctg ccggcattct caagtgacgt ggccgtaggc
     3001 cggctggctc gcgggtgcgc catcacttag cctgcgccaa aggcgacgta gccagcgccg
     3061 ctgatcaaga agtcgagcac gatcactgcg agggacgagt tgacgaccgc gcgcgtggtc
     3121 gcgagcccga ctccttcgga gccgcccgac gtcatcaatc ccgcgtgcgc gctgatgatg
     3181 ggaatcgctg cgccgtacgc aacgcacttc gcgatgccaa ccactacgtc cgaaacgtcg
     3241 actaggctga ggcttgcgaa cgtcgcggga cggatctcga agaagacgta cgccgcgccc
     3301 aggcccgttc cgaacgccac gctgcctgcg atcaccacga ggaccaaggt catgcacagg
     3361 catgcgatga agcgaggctt gacgaggtag tcgattggat cggccgcgca catgcgcagc
     3421 gcatccaatt gctccgtcac caccatggac ccaatctctg cggcgattcc ggcgcctacc
     3481 cgagttgcca gcattagagc gcaaatggtc gctgcgagat ccttgaccaa aagctctaga
     3541 aaggtgggtc ctaacccgct cagatccggc actacgcgct gggtttggag cccgccttga
     3601 taaaccagga tcgtgccgat gaagccgagc acgacgcaaa cgaacacgag cgagcggttg
     3661 ccgatctcgt acatctgtcg gagtacggag ccgcgctccg aacgcccacg gaacacataa
     3721 tagagggtgg tagcgaatgt accgtacagc gccttgaaga agtccatgcg cggtcagatc
     3781 cgagtgctgt gccgggaatg ggccagggtc atcccaaggc aaatgacgat aggtagtcgc
     3841 aagcgaagat gcccacggca ctggtcacga ccgtcgcatt gacggcccgc ccaacacccg
     3901 gagccccccc ggcagtcgat atgccgaagt gacagctgca aagcccgatg atgcctccga
     3961 agagcacgct cttgatcagt ccgtgcagga cgtccccgaa gccgagcagc tcgctggtca
     4021 ggccttgaaa gaagaccgtg ggctccacgc ccaacaaggc ccagccggcc tgagatgcac
     4081 cgaacagcgc gagggcgtcc gagaagaccg tcgagagcag cagcgtgagc acgattccga
     4141 aaaatcgggg cgccaccaga tagccttggg gatcgatggc caacgcgcgc agcgcgtcga
     4201 gctgctcggt gacggccatg gtgccgagct cggcggtgtt gttggcgccc acacgcccag
     4261 agatcatgag agccgtcagc aacggcccaa tttcacgcaa ggtgccgaag cccgcacccc
     4321 agcccagcag cccctgagcc ccgtagcgct ccacgagtgg ccccgcctga atgaccatga
     4381 tgccgccggt gaacagcgcc gtcgtggcca caattggcag actcttcacc cccattcggt
     4441 gcaggttacg gatgaactcg agcccgtcta gctcccgccg tagtgctgcg ccgcagatcg
     4501 acgcgaacag caaagccatg ctgccgagca gccgcgcctg ttcggagaga gcgagggcgg
     4561 ctcgggtgag cgcgccctcg ggcgcttcgg aagggaccag ctcggccatg cgcggtcagt
     4621 gtacacggcg ggccttggtt cgtgagactt tcaggaggcg agttgatcag cgagcgcgtg
     4681 cggtttgtct ttggctcctc gcggcgagcg tgtaggttcc gcgcccgacg tgacgcaaga
     4741 cctccgaatt ggcgtaattg ggctcggata cgcgggcacg cggcatgcgc gggcgctgca
     4801 agacgggcgc atacccggag cgcggttgca cgccgtgtgc gaccaggacc cggggcgggc
     4861 cgcggcattt ccagactcgg tgcacgccag gttggacgac attttcgccg agccggcgct
     4921 ggacgcagtg gtaatcgcga ctccgcaccg aagtcacgtg gagctctgcg agaacgcgct
     4981 ccgtgagggc ctatcggtcc tggtcgagaa gccgttcggc gtgcacgccg ccgagtgccg
     5041 gcgggcgttg gctgctcatt cggacgcgat tcgttcggca gcgctccatt cagcaacgaa
     5101 gcgaacggag acagcaccgc aagagcaggt ttcagcgcgc attcagcgct ccgtgatgcc
     5161 tctcctattg ggcgtggtgc acgactaccg cgtcgaaccc aagttcattt ggctgaaaga
     5221 gctcatccaa cggggtgaac tcggacgagt cgtgcgcttc acctggcaag ccaccaattt
     5281 tttccgcacg gaagcgtact tccagacaag cccctggcga gggcgcttcc tcactgaagg
     5341 cggtggcatt ctggtcaatc aagcgccgca cttgctggac gcggtggcct ggttgtttgg
     5401 ggtgccgcgt cggctattcg caagctgtgg cttcggtcgc tatcaccaga tcgaagtcga
     5461 ggacgacgtg aatgcgctgc tcgagtacgc cgacggcacg cgcggcaatc tacagttgtc
     5521 cacgggtgag agcccgggta cgcagcggct ggatgtctcg ttcgacggag gtcgggtcgt
     5581 cgtcgaaggc aatgaggcgg tcattca
//
LOCUS       DASTKU010000584        24271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1004939, whole genome shotgun sequence.
ACCESSION   DASTKU010000584 DASTKU010000000
VERSION     DASTKU010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004939"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFQ61_20385"
     CDS             complement(<1..801)
                     /locus_tag="VFQ61_20385"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9956871.1"
                     /translation="MAFRQPIPRRALLRGAGVCMALPFLEAMLPARAQAQTAATPKRF
                     VAFFYPCGTDPRKWKPAAGPLSAASLPECLKDLTGFAAEGIWPAGDGILADVTAVTGI
                     DHSGVCTDIHVPSMALSAHKGVKNNYTPPEPTLDQYLAENLKASTPYRNLALSATGST
                     DIAQGNISFRAGGQVETVVRNPKQLFDNLFGKTTTTGGSSSAPDKAQKRRASILDLVK
                     ADVSRLNARVGSADRQRLAQYLQSIGELEKQVNTSTPTTCTAPAAPASG"
     gene            complement(821..2512)
                     /locus_tag="VFQ61_20390"
     CDS             complement(821..2512)
                     /locus_tag="VFQ61_20390"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9956872.1"
                     /translation="MPSPSDSGNSTGANAGNAGIGASVGNVPGGGTGITASNAGAPNS
                     GSTSGAGNATGGAALASRCADPNAATADAGESVLKRLSAIEYQLSVQDLLALPNAPSI
                     EGIPADTAKDGFKTFAEVQTVSAQHLRAYLDKASELSSALMADAARRSKVLGCEPSAK
                     DCLKSFIARFGKQAYRRALESDEVDSLSSYAQTNAVDANDQFQYVIEAVLTSPNFLYR
                     VEVGKPAEAVTALTPTELASRLSFSIWGRTPSAELLARAEQGTLSTAEGLRTEVQGML
                     NDPRAQSFYGGFFRQWLGYETLRAPTKALTGWSEALMPAMQAETDQVLNDYAWGKRNF
                     LEVLTTNRSRVSADLAKFYGAPAPGADGSVEFPQTHVRANTGLLTHGSLLSAKSDGDL
                     IAIRGNWIRRTFLCRSMEVPASVAEQLGELLVGLTRVQIVARRNTEAACKGCHAVIDP
                     IGVGLSAFDQSGRFDATFDSTVFGIAPALPDAPMPAFATVAELSAKLRGMPEVAECLS
                     EKLFLYVNGREATANDACTLASAQTAFQSGGQDFAALLSGLIQAPGFRLRRAPSL"
     gene            3373..4215
                     /locus_tag="VFQ61_20395"
     CDS             3373..4215
                     /locus_tag="VFQ61_20395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9956873.1"
                     /translation="MTRRFGAALALSNVTVSFSPGEVHVLVGGNGAGKGTLIKILSGA
                     DSDYRGELWLEGAKVRFRNPSQARALGIACIYQELSLVPNLSLVENIGLRRSSRSSFC
                     PSLFAGAARGAAREEAKRLRCLMQLEVDLDREVRAFSLAEQQLIEVGAALLEEVGVLV
                     LDEPTSALSEREAECLFEHLQRARAAGTSVIYISHRLEEVYRLADRISVLRDGELVLT
                     AWASDLPQPELVRAMVGVVGQRPPRRGDEAAERSAASRPNQCFASTHWNDPVSRDWKM
                     FGCR"
     gene            complement(4309..5286)
                     /locus_tag="VFQ61_20400"
     CDS             complement(4309..5286)
                     /locus_tag="VFQ61_20400"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HET9956874.1"
                     /translation="MAFKIRAGEPIQSAAARTARKQLARAVEALDDPALPLADKVHEV
                     RTTIKKVRALLRLVRPRVGKHFVKPNRKLRDLARKLSPLRDAQVLFDTCARLASAAPH
                     QAKPEFSRILEALAHQLAQATAEFLSNRDALPELRKNLLRARRRVGTWATHARGYTAL
                     GPGFIVSYSRARDRMRELAPHSSGAQYHEWRKAIKTYRYQFGLLEVMWPVAMREQAEK
                     LEKLGELLGHEHDLSVLESRLGSHAPGDPSAARADAAQLVSPRSHAESAALGADPESA
                     SPLREQLREERDRLRQQARELAAPLFDESPDELARRVRTYYRIFKRTKP"
     gene            6200..7702
                     /locus_tag="VFQ61_20405"
     CDS             6200..7702
                     /locus_tag="VFQ61_20405"
                     /inference="COORDINATES: protein motif:HMM:NF014948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956875.1"
                     /translation="MTKKVALFWPGDGRAKPNELALPNMEAATQQMERALRKLGREPY
                     RVPGFLSKPHEALDRLAGVDDPLVGMFVHWVYGPHTTEGVVGKQNPLLLASNFSGTWP
                     GLVGLLNTGACLEMLNREHSRIWTSAEDFSSDALFMERLDAWCSSGRIDYPTDGLRSH
                     APVSVEASRRASEVAVAIRKRRPLVLMLGDTSMGMINGYFGPRLLNRHAFSEHKIDQA
                     WIIDRGRRIEDRRIDDALAFVKDRGVTFHYAGDFDERATREQLRDYLAVLDMIGEFGA
                     DCLGWQYQLGLIPLRPPSDFAEGLFNSVARPESNGDTIACATEADQGNVLPMEMLKRL
                     LKSKGLHQAVMFHDVRWGAEHEGRFLWVLLNSGSCGAYAFNHDPQTLKGVHSYRQPAL
                     YFPTAGGTFAGESLAGRMTWARAYIKQDVLWMDVGRGEVVKLPPEKRDAWWQGTTREW
                     PFMAADMGVSRDTLMAHYQSNHVAVAYGDVYDEMIALSLELGFRVRVLGA"
     gene            7733..9406
                     /locus_tag="VFQ61_20410"
     CDS             7733..9406
                     /locus_tag="VFQ61_20410"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET9956876.1"
                     /translation="MSEQASGEDLYLGVDVGTGSVRAALFRADGQRMGLGVEVIQLYR
                     PAEDFVEQSSDDIFRAAGVAIRQALSEAHARPEWVRGMAFDATCSLVALDAQDHPVSV
                     SPTGDDRNNVVVWMDHRAVEQAARINQVPHEVRRYVGGQISPEMQLPKLLWLKEHAAA
                     SFARAARFLDLADFMSYRATAVDVRSLCTTVCKWTYLGHESRWADDFLVAIGLPGLAS
                     VGQAKIGQRVRPMGERIGTLTPRAAAELGLTSGTVVGVGIIDAHAGGLGLLGTPVAGQ
                     VGQALDTATLSRRLALISGTSSCHMAVSPEPRFVPGVWGPYFSAMVPGLWLTEGGQSA
                     TGALIDHVIHSHARGAELAERALREGTSVYTLLNRELARLSPESDPRLTSELHVYPDF
                     HGNRSPRADATLRGMVSGLKLSDSLDSLALLYLATIQGIAHGTRHILWALEQQGYRIE
                     TILACGGDTKNPLFLREHADITGCTIALPREPEAVLLGAAMLGSVACGRYATVVDAMR
                     AMSAPGSSVAPRPTFAAYHERKHEVFHRMYLDQLAYRDLMESAPHKILP"
     gene            11149..12420
                     /locus_tag="VFQ61_20415"
     CDS             11149..12420
                     /locus_tag="VFQ61_20415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9956877.1"
                     /translation="MPKFALVVCVSALAFAAGCSSDSGDGASFAGGGIPGLPPNQIPG
                     AGTGSTGAGGGNIPTPGGPLSGGQIAISPQQVQTIKQQSCAGSSVEGETVPSVLQLVV
                     DVSSSMNDQAPGSNRSKWEVTRDALINAIPGRSGNAGLSPSLGVGLLFYPNRHVDVST
                     TPVATDQCVNTSANIVPALLGPANAQHRTLIRSSFEQVQLQSSTPTLDAYRYAFQNSI
                     TKSTLTGQKYMLLITDGTPTLALGCMNPSGGLSDVSAADIEAIVSEVKAARAAGIKTF
                     LIGSPGSERNRNWLSRAAIEGGTAPAGCRADGPNYCHMDMTTAPDFGKALQDGLGKVV
                     GQIAPCNYSFPPPPNGETLDSNKINVIVTSGGQSQLVVRNDVGNCTEGWELTDSQEIR
                     LCPSTCNAIQQDASATVDVVFGCASLSEPPR"
     gene            complement(12518..13450)
                     /locus_tag="VFQ61_20420"
     CDS             complement(12518..13450)
                     /locus_tag="VFQ61_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9956878.1"
                     /translation="MTTIWRRPWFGPLASLLALYVLFSVLSPDTFLGAANLLTMTRQT
                     VVVAITAVGMTYVIILGGIDLSVGSAVALTTVALAWLLKSGYGPLSASALAALVPALS
                     GAVNGAFIAGLRIPAFIVTLGTMSLLRGLAKGISGEQKIDANPLGIDTWMSATDGVGL
                     PPGVWFLAASAALAAVALNSTVFGRHVVAIGSNEQTARLCGVPVARVRVLVYGLAGLL
                     AGLAGVLEYATLTVGDPTDSIGLELEVIAAVVIGGASLSGGQGSIVGTLVGALLMTEI
                     KTGCTHVGVPNWVQEIFTGAIIVAAVGIDRLRRK"
     gene            complement(13447..14928)
                     /locus_tag="VFQ61_20425"
     CDS             complement(13447..14928)
                     /locus_tag="VFQ61_20425"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET9956879.1"
                     /translation="MRLSARGIAKSFGATRALSDVALEVAPGSIHAVLGENGAGKSTL
                     MRILAGAMQPDEGQMLLEGKAYSPRDPLSARGLGVAIVYQELSVCTHLTVAENIVLGV
                     EPSRWGWLSRREMRTRAERALTWVVPSRSRIGPDTLLADLAPSDRQLVEIARALCAES
                     TKLLILDEPTSTLSGDATERLFAALARLRERGLSILYISHFLEEVRRIADEYTVLRDG
                     RTVARGRIAEVTNRDLVSAMAGQAIAERVREARPKGRVLLRLEELAGTALPVSASLEL
                     HAGQVLGIAGLVGSGRTELLRAIFGLAPVLRGEITVGAWPGAASPAVRLQQSVGLLSE
                     DRKLEGLALELSIRENLTLSRLAPTQKHGLLSRAEERKICSAWIERLGVRCRGPDQSI
                     QELSGGNQQKIALARLLYHDVDVLLLDEPTRGIDVRSRAEIHGWIDELSSRGKGILMV
                     SSQLPELFSVCDDLCVMHRGQLGPVRPIRSLTEAGVLQEATGA"
     gene            complement(15023..16069)
                     /locus_tag="VFQ61_20430"
     CDS             complement(15023..16069)
                     /locus_tag="VFQ61_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9956880.1"
                     /translation="MRAFAKQGEVTVRREFLKQVIGLSAIWLSSCQKTQGQQTTDKAA
                     SGRLKLAVIPKGTTHEFWKSVHAGAVKAARELDVDIVWKGPLKEDDLKSQVDLVESFT
                     AQKVSGIVLAPLNDKALVSSVVRSTQQKIPVVIFDSALSGGNTVSFVATDNRAAGRLA
                     GQRMLQLLQGGPGKVVVLRYQEGSASTHEREEGFLEAVKGKPGIEVVSDNQYGGATTE
                     SAFSASESLLLAKAADKGGVQGVFAPNESTTFGMLLALEKAKLSGKVHMVGFDASEKL
                     LKALNSGAIDALVVQNPFNMGYLAVKSLVAHIKGQSVAPRTDTGAQIVDKANLESAEI
                     QELVKPDLAKWLSP"
     gene            16351..17022
                     /locus_tag="VFQ61_20435"
     CDS             16351..17022
                     /locus_tag="VFQ61_20435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956881.1"
                     /translation="MVRRFARLGSAPLFALCSKTGAAPCAKPGAALCAAITLAAALSA
                     PHEAHAQALREPGKVAISIERAFGINYSHTEIDKPLHAERSDTTLGFGFQPGLSPLHW
                     ARFGVDVFINEHWSVGGSVGFFKVSGDTDASGLLLAPRVGYALPLGKLVTFWPRAGFS
                     YVKPGDAHLIGLSLEGMFVISANPAWGVLVGPTLDVGISGKSSGGDWSELAVGIPTLG
                     LVATF"
     gene            17046..17324
                     /locus_tag="VFQ61_20440"
     CDS             17046..17324
                     /locus_tag="VFQ61_20440"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET9956882.1"
                     /translation="MPRLNFTPNLERHLGTRSLELPANCGDTLREVFDAAFARLPLAR
                     AYILDDQGGVRRHVAVFVDGQLVSERQRLAASVASDAEIWIMQALSGG"
     gene            17367..18512
                     /locus_tag="VFQ61_20445"
     CDS             17367..18512
                     /locus_tag="VFQ61_20445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956883.1"
                     /translation="MKIPQLFVATRKGLFTIAKHLGAWDIVDVSFEGDNVSMVLPDTR
                     DGAVYVAIQHGHFGSKLHRSDDDGKTWKELTAPQYPPKPESEVDKDPIRGEDVAWSLQ
                     LIWSLEAAGDQRIFAGTVPGGLFYSDDRGESFNLCESLWLRPERRRWFGGGLDAPGIH
                     SIVVDPADGNHVLIAVSCGGIWRTRDGGGSFTLEGKGMHASYMPPERRQDLDIQDPHR
                     LVQCASKPEVLWVQHHNGIFRSTDYAESFVEISGAKPSSFGFTVAAHPKDPEVAWFVP
                     AARDDRRIPVDRRVAVMRTSDGGRSFEPLTRGLPDQFAYDLVYRHALDVDATGALLAF
                     GSTTGNLWISENGGDAFATVSQNLPPIYAVRFQHPHAAPALERVQAA"
     gene            18519..19178
                     /locus_tag="VFQ61_20450"
     CDS             18519..19178
                     /locus_tag="VFQ61_20450"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET9956884.1"
                     /translation="MIDIPPIWRVVRPLAELIDSPEAAGRAADLQPARTKPAAAAPPR
                     IQLVISPGAGFGRGDHETTQLCLQAVHWFRPPGAGGWRMLDFGSGSGILAVAAAKLGA
                     SVEAVEIDDAAIAHAERNACLNGVQGRILYSKSLEPLSGVFELVVGNILLEVLLAHAE
                     ALVARVAPGGRLVLSGLVATDVPAVIARYAPLLDQSRPDVYERAPWRLLTWRRGPSDA
                     G"
     gene            complement(19317..20312)
                     /locus_tag="VFQ61_20455"
     CDS             complement(19317..20312)
                     /locus_tag="VFQ61_20455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956885.1"
                     /translation="MHLTSSNRSSRAASRFAFAGPSLRFAAVTLMTVACSASSDPVEH
                     KDGTGGSSPAATGGANGNTSGATGFSGSGFGGFTSAQGGVTSAQGGATSATGGAATGG
                     ATSAAGGTTAANGGATVVANGGTTATGAGGTTATGAGGTATSSCATYSGTLKAASTIF
                     MEGYGTSGTWMGYAYTYKYGTATITPGSSQTQSCFPGAVLCAAGSVPATYDDGAGIGW
                     SINQAKAGGTPDTVALTGTVKVQLAGATAGMRVGLQPAGTAAEFCYELAAADVTAANG
                     TGLSLPVSSFKSKCYDTATAVAYAGEPIQAIQISIPGTKDAVRAFDVCIVSISGG"
     gene            complement(21125..21472)
                     /locus_tag="VFQ61_20460"
     CDS             complement(21125..21472)
                     /locus_tag="VFQ61_20460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956886.1"
                     /translation="MRGWSDSCRNQSQAPGAALQVARSSRSLYLAEQYLPERRQMEQC
                     RVVATSSDLLAKSTHRGLAPHWGTRHATVRLGWWVLAQVLQEGRLAWPPWVELALDQQ
                     PARGSYWQGRAGC"
     gene            21478..22182
                     /locus_tag="VFQ61_20465"
     CDS             21478..22182
                     /locus_tag="VFQ61_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956887.1"
                     /translation="MTPVVTPEPPSATYEEEEDPGRGMRAGALPELEEIRLAKPEGVA
                     FGLDGYGGIAVLSRQSTGASHAYLGGLARVRVGYLQFGGSFEQSDVVDNEWRTIGGFV
                     GAFFPFHNWVDFDAAVGFASRRHASSDGRYGPDGYNISMPAMTLRFGVSDRTSESLSG
                     VRVGAYLMTTIDLKQRRVPWSFSIENSSGEEVVTTGSSRVGGFSIGGAFALGFDIVPG
                     AHDAPPVPPKSAEPGR"
     gene            complement(22192..22947)
                     /locus_tag="VFQ61_20470"
     CDS             complement(22192..22947)
                     /locus_tag="VFQ61_20470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956888.1"
                     /translation="MSLHSSWSPDAAQVLARACEYAGGATAWNDAQTIRLHPVENGLT
                     GLVPRLKGLGRTFVMPEYFDVMPHQRCARFVGYPAPNSVGIFENGAVRIEGPAGVSSR
                     SELHRRSFRGLSKYRRWSALDALYFFGYALTHYHALPFTLREATLKSSYRERGRGRTL
                     DVLEVELPEDVPSHCRQQRFYFSEAGQLVRNDYHAEILGFWARGAHYWRESTRVNGLT
                     VCLERYVLARVGRLTLPLVALHARFARAELIPG"
     gene            23240..>24271
                     /locus_tag="VFQ61_20475"
     CDS             23240..>24271
                     /locus_tag="VFQ61_20475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9956889.1"
                     /translation="MRHPMLLLLLAYLAFVSLGLPDTALGVAWPSLRRHFDLPASAIA
                     TVLTAGMAGYFTSGLLAGKLMQALGVGGVLALSCGFVTAALTVYALAPAWWFFFPGGL
                     LMGIGSGAIDAGLNGYAARHFSVKHVNWLHACWGIGASVGPALMTGAIARGNGYTAGY
                     GLLALALGGMTVAFAWTHRLWNEPAGEVRTARSTEAQADAAVQAHAEVSAAGDGPRVT
                     MFAALRSGPVWLHIVAFFLYTGFEASTGQWCFTLLTAGRGLSVERAGFWTSCYWGSLT
                     LGRVLLGFVVDRFGPDRLLRYATTSAALGAAAWALDPGLVGRLGLLLVGLSLAPIFPT
                     LMARTPARLG"
BASE COUNT         4302 a         7527 c         7824 g         4618 t
ORIGIN      
        1 gcccgacgcg ggtgcagcgg gtgccgtgca cgtcgtcggc gtgctcgtgt tgacctgttt
       61 ttctagttcg ccaatggatt gtaagtattg tgcgaggcgc tgcctgtcgg cgctgcccac
      121 gcgcgcgttc aggcgactaa cgtcggcctt caccaaatcc aagatgctgg cgcggcgctt
      181 ctgcgctttg tcgggcgcgc tgctggaacc gccggtcgtg gtggtcttcc caaacaggtt
      241 gtcgaacagc tgtttcgggt ttcgtactac ggtctccacc tggccgcccg cgcggaacga
      301 aatgttgccc tgagcgatat cggtgctgcc ggtcgcggac aacgccagat tgcgataagg
      361 ggtcgaggcc ttgagattct cggccaggta ttgatccagc gtgggctcag gcggggtgta
      421 gttgttcttt acgcccttgt gcgcagacag cgccatggaa ggcacatgaa tgtccgtgca
      481 cacgccggag tggtcgatgc cggtcactgc cgtcacgtcg gccaggatgc cgtcccccgc
      541 cggccaaatg ccctccgccg cgaagcccgt gagatccttc aagcactcgg gcaggctcgc
      601 ggccgagagc ggacccgcgg ctggcttcca tttgcgggga tcggtgccgc aggggtagaa
      661 gaaggccacg aagcgcttgg gggtagcggc ggtttgagcc tgcgctcggg cggggagcat
      721 cgcctccaag aagggcaacg ccatgcatac gcctgctccg cgcagaagag ctcttcgagg
      781 aatgggttgt cggaatgcca tgaggagatc ctgctaacgc ttagaggctg ggagcgcgcc
      841 gcaggcggaa gcctggcgct tgaatcaggc cgctcaagag cgcggcgaag tcttgccctc
      901 cgctctggaa tgcggtctgg gcggaggcca aagtgcaggc gtcgttggcg gtggcttcac
      961 gcccgttcac gtacaagaac aacttctccg acaagcactc ggcgacctcc ggcatcccgc
     1021 gtaacttggc ggagagctcc gccaccgtag cgaacgcagg catgggcgcg tcgggcaggg
     1081 ccggcgcaat gccaaacacc gtgctgtcga acgtggcgtc gaagcgcccg ctttgatcga
     1141 atgccgatag cccgacgccg atcgggtcta tcacggcatg gcagccctta caggccgctt
     1201 ccgtgttgcg gcgcgctacg atttgaactc gagtaaggcc gacgagcagc tcgccgagct
     1261 gctcggccac ggaggccgga acctccatac tgcgacacag gaacgtgcgc cgaatccaat
     1321 tgccgcgaat ggcgatcagg tcgccatcac tcttggcgct cagcaaactg ccgtgggtca
     1381 gtaggcccgt gttggcgcgc acgtgggtct gcgggaactc gacgctgcca tctgcccccg
     1441 gcgccggagc gccgtaaaac ttcgcaagat ccgcggacac ccgcgaacga ttggtggtga
     1501 gtacttcgag gaagttgcgt ttgccccagg cgtagtcgtt caacacctga tcggtttcgg
     1561 cttgcatggc gggcatcaac gcctcggacc agcccgtaag ggccttggtg ggcgcgcgca
     1621 gcgtttcgta gccgagccat tgtcggaaga agccgccgta aaagctctgt gcgcgagggt
     1681 cattcagcat gccctgcacc tccgtccgca agccctccgc cgtgctcagc gtgccctgct
     1741 ctgcgcgcgc caacagctcc gcgctcgggg tacggcccca aatcgagaac gaaaggcgac
     1801 tcgccaactc ggtgggggtg agcgcggtga ccgcctcggc gggcttgccg acctcgactc
     1861 gatacaagaa attcggagag gtgagtacgg cctcgatcac gtactgaaat tgatcgttgg
     1921 catcgacggc gttcgtttga gcgtaactcg agagcgagtc cacttcgtcg ctctccaacg
     1981 ctcgccggta tgcctgtttg ccgaagcgcg cgatgaagct cttgaggcaa tccttggccg
     2041 aaggctcgca gccgagcacc ttgctgcgcc gcgcggcatc cgccatcaac gcgctggaga
     2101 gttcgctcgc cttgtccaga taggcgcgta agtgttgcgc cgaaacggtc tgaacctccg
     2161 cgaaggtctt gaaaccgtcc ttcgcggtat ccgccgggat gccttcgatg cttggcgcgt
     2221 tgggtagcgc cagcagatcc tgcacgctca actggtactc tatggcggag agtcgcttta
     2281 gaacgctctc ccctgcgtct gcggtagcgg cgtttggatc cgcgcagcgg ctggctaggg
     2341 cggctccgcc ggtcgcgttt cccgcaccgg acgtgctccc ggagttcggg gcgcccgcgt
     2401 tcgaggccgt gatccctgtg ccgccaccgg gcacattgcc tacgctcgcg ccaatcccgg
     2461 cgttgccggc attggcgcct gtcgagttcc cactatccga cggcgacggc acaccaggag
     2521 ttgcgccgct cgaagccggc gcacctgaat cggcgccaac ttggccaaca caggctggtc
     2581 caaagcaccc aagcgtgctt gctaccaagc agagcagtcc cacgcgagag ccaactgcgc
     2641 gagagtcagg ccgacgcgag cgcgtcgaga gacactcgga gctacccgag aacaccgggt
     2701 gggaagagtg gagtccgatt cgcacctgtg ttcgctcctt gaagtcgaag gatagccgag
     2761 tcactcacta gctcaaccct ggagttcgag ccagagggcg aaattcgata tttgcaccgc
     2821 agggacacaa agttgtgtgc tcggcggatg gatgcggttc gatgaagttc gacacgcagt
     2881 gattggaaga acgcttctct catcggagat gcattctccc tacggtttcc cggcctcgtt
     2941 ggccccacgt cagtatggcc gcgagtgcgc cacaggccgc cttgctcgcg acggttccat
     3001 ccaacccacc aagacacacc gaacgtcgtg tttcgcccat accgatgctc acgatcgata
     3061 ttcttaccgc gcaactgctt atttgcgcct tacgcgggtc tcgagtgttg cttcgagaac
     3121 cctgggttgg ggttccggat tgggcggggt tgcgccgagt tcatgaaacg aaaatcgagc
     3181 tccttgcgat tcacgcaaat tccagaaatc tgcggcccct catctgcgtc gggagcgtga
     3241 tgcggtaaac ttctcgatag cgtggctgtc caattaccga ctgcatcacc gcgcgatctc
     3301 ccgccggttc ttccgcgtgc tcatcctcac gccgcggggc cagcgcctac gcatcgcctg
     3361 gaattgcgcc acgtgactcg tcgcttcgga gccgcgctcg cattgagcaa cgtcactgtt
     3421 tctttctcgc cgggggaagt gcacgtcctc gtcggcggca acggcgcagg aaaaggcacg
     3481 ctgatcaaga ttctgtccgg tgcggattca gactatcgag gtgagctttg gctcgaaggc
     3541 gcgaaagtcc gttttcgaaa tccgagccag gcgcgagctc tgggcattgc gtgcatctac
     3601 caggagctct cgctcgtccc gaacttgtca ttggtcgaga acatcggact tcggcgcagc
     3661 tcccgctcga gcttttgccc gagtctattc gccggcgcag cgcgcggggc cgcccgtgaa
     3721 gaagcaaagc ggctgcggtg cctgatgcag ctcgaagtcg atttggaccg cgaagtgcgc
     3781 gcgttctcgc tggcggagca gcagctgatc gaggtcgggg ccgccttgtt ggaggaagtg
     3841 ggggtgttgg tgctggatga gcctaccagt gcgttgtcgg agagagaggc ggagtgcttg
     3901 ttcgagcacc tgcaacgcgc aagggccgcc ggcacgagcg taatctacat ctcgcatcgg
     3961 ctcgaggagg tctatcgtct cgccgatcgg attagcgtgc tccgagacgg tgaactcgtg
     4021 ctcacggcct gggccagcga tcttccgcag cccgagctgg tccgcgcaat ggtcggcgtc
     4081 gttgggcaaa ggccgccacg ccgcggagac gaggcggctg agcgctctgc tgccagtcga
     4141 cccaaccagt gctttgcatc gactcattgg aacgatcccg tgagccgcga ttggaaaatg
     4201 ttcggttgca ggtagggtcg gggagatcgt gggttggtgc ggccggcgat gatcttcggc
     4261 ccgagtgcgt gatgggtcaa gcgttgcgtc ggggctctcg gagtgggctc agggcttggt
     4321 gcgcttgaag atccgatagt aggtgcgaac tctacgcgcg agctcgtccg gcgactcatc
     4381 gaacagcggc gcggcgagct ctcgcgcttg ctgacgtagg cgatcgcgct cctcacggag
     4441 ctgctcgcgg agcggtgacg ccgattccgg gtccgcgccc agcgcggctg actccgcgtg
     4501 cgagcgcggc gaaaccagct gcgctgcatc ggcgcgcgcg gcgctcgggt cacccggggc
     4561 gtgggaaccg aggcggctct cgagcacact gaggtcgtgc tcgtgtccga gcagttcgcc
     4621 gagtttctcc aacttttccg cctgttctcg catcgctacc ggccacatca cttcgagtag
     4681 cccgaactgg tagcggtacg ttttgatggc ctttctccac tcgtgatatt gcgcgccgct
     4741 gctatgcggc gcgagctcgc gcatgcgatc gcgcgctcgc gaatagctga cgatgaagcc
     4801 ggggccgagc gcggtgtagc cgcgggcgtg agtggcccag gtgcctacgc gacggcgagc
     4861 tcgcaacaaa ttcttacgca gctcgggtag tgcgtctcga ttgctcaaga actccgccgt
     4921 agcttgggcg agctggtgcg cgagggcctc caaaatgcgt gaaaactctg gctttgcttg
     4981 gtgcggggcg gcgctcgcga gtcgcgcgca agtatcgaac aagacctgcg cgtcccgcag
     5041 cggcgagagc ttgcgcgcca ggtcgcggag ctttcggttc ggcttcacga agtgcttgcc
     5101 cacccgtggt cgcacgagcc gcagcagagc acgcaccttc ttgatcgtgg tgcgtacctc
     5161 atgaactttg tccgctagcg gcaacgccgg gtcgtcgagc gcttcgacgg cgcgagccag
     5221 ttgtttgcgc gcggtgcggg ctgccgccga ctggatgggc tcccctgctc gaatcttgaa
     5281 agccaaaacg aaagatctcc ttgctgcgaa gttggggtgg tgcgggagga tggcggatgc
     5341 gtctcgaacg ctgccatcca agtatggtct agagcagagg catctcacct ggtggtgacg
     5401 tggtggtggc ggctacattg ctttgtggcg tcagggccca cgccgcggag gcactctgtc
     5461 tactcccgat cccgaactct cgcagcatga tcccgaactg cgtcggcccc agcctcccgc
     5521 agccggggag cggctcgccg gacgcacggg gttcggctca ttccaggttc ttagcctgga
     5581 cgcctacagc agcgagctgc gcttgatgtc gacaggaacc attgggatcg tgccgtgctt
     5641 ttcctacgca gaatgagcga acgcgctagc tgagctcgga gtgtggccaa agccgcggca
     5701 agcccgcaga ccgagccgtg tagctttacg cgacgagagt acgacggccg aaatgagcga
     5761 acgataggct tctctcgcgg tagtactagg aaccggtgag gcggcttcgt atagtggcga
     5821 gcatgacgaa gaccatgcct gacggttcta ggtctattcg tggtgctcgc gactgttgcc
     5881 tgcaaaaaag agtcgcgttc ggagtcgggg gtgggtgcgg tgtcgggggt gggtgagcgc
     5941 gcgttagcaa agcccgtgtt aatcggtgtg agccagcgca attgggcgga accgtgctcg
     6001 atactagcct tgcgcgctca caacgaccct ggcgcgcacg gcgcatgaaa acgtggatgc
     6061 gggcggcgaa gccctgccgt gaagggctgg gtccgtatcc atggcgcggc ggcgaccgta
     6121 cggccgccga gaaccgaccg tattcacgag cacgactgac tgaacgatcg attcagccca
     6181 aaccggagac agcaaataca tgaccaagaa agtggcgcta ttttggccag gtgatggccg
     6241 agccaagccc aacgagctgg ccctacccaa catggaggcg gcgactcagc agatggagcg
     6301 cgcgctgcgt aagctggggc gcgaacctta tcgggtcccg ggcttcctca gtaagccgca
     6361 cgaggcgctc gatcgcttgg cgggcgtcga cgatccgctc gtcggcatgt tcgttcactg
     6421 ggtgtatggg ccgcacacca ccgaaggggt ggtcggcaaa cagaatccct tgctgctcgc
     6481 cagtaacttc tccggtacgt ggccaggctt ggtggggctg ctcaacacgg gcgcatgctt
     6541 ggaaatgctc aatcgtgagc actcgcgcat ctggaccagc gccgaagatt tttcgagcga
     6601 tgcgctgttc atggagcgcc tcgatgcgtg gtgtagctcc ggccggatcg actatcctac
     6661 cgacggtttg cgcagtcacg cccccgtttc cgtggaggcg tcccgccgag cctccgaggt
     6721 ggcggtcgcc atccgcaagc gtcggccgtt ggtgctcatg ctcggtgaca cctcgatggg
     6781 catgatcaat ggttacttcg ggcctcgact cctgaaccgg cacgctttct cggagcacaa
     6841 gatcgatcag gcatggatca tcgatcgggg cagacgcatc gaggatcgcc gcatcgacga
     6901 tgcgctggcg ttcgtgaaag atcgcggggt gacgttccac tatgctggcg atttcgacga
     6961 gcgggcaacg cgcgagcagc tccgcgatta cttggcggtt ctcgatatga tcggggaatt
     7021 cggggcagac tgtctcggct ggcaatacca gctcgggctg atcccgctca ggccgccgtc
     7081 ggacttcgcc gagggtctgt tcaactcagt agcccggccg gagtcgaacg gggatacgat
     7141 cgcctgcgca accgaagccg accagggcaa cgtgctgccg atggaaatgc tcaagcgcct
     7201 cctgaagagc aaagggctcc accaagccgt gatgttccac gacgtgcgtt ggggcgccga
     7261 gcacgaaggg cggtttcttt gggtgctcct gaattcgggc tcgtgcggcg cctatgcctt
     7321 caatcacgat ccgcagacgc tgaaaggcgt gcactcgtat cggcagcccg ccctatactt
     7381 tccaactgcc ggcggcacct tcgcgggaga gagcctcgcg ggccgtatga cttgggcgcg
     7441 cgcctatatc aagcaagacg tgctctggat ggatgtcggg cgcggagagg tcgtgaagct
     7501 gccccccgag aagcgggatg cctggtggca gggcacgacg cgagagtggc ccttcatggc
     7561 ggccgacatg ggtgtgagcc gggatacgct catggctcac taccaatcga accacgttgc
     7621 cgtagcttac ggggacgtct acgacgaaat gatcgcgctt tcactcgaac tgggcttccg
     7681 cgtgcgtgtg ctgggcgcgt gacggccgaa ccgaatccga gctgaggaca tcatgagcga
     7741 gcaggcgagc ggcgaagacc tatacctggg cgtagatgtg ggcactggca gcgtgcgcgc
     7801 ggcgctgttc cgcgcggatg ggcagcgcat ggggctcggc gtcgaagtga ttcagctgta
     7861 ccgccccgcc gaagatttcg tggagcaatc gtcggacgac atctttcgcg cggcgggggt
     7921 ggcaattcga caagcattga gcgaagcgca cgcgcgcccc gagtgggtgc gcgggatggc
     7981 cttcgacgcg acctgctcgc tcgtggcgtt ggatgcgcag gatcatcccg tcagcgtgag
     8041 ccctacggga gacgatcgga acaacgtcgt ggtctggatg gaccatcgag cggtcgagca
     8101 agcggcccgc atcaatcagg ttccacacga ggttcggcgc tacgtaggag gccagatctc
     8161 gccggagatg caactaccga agttgttgtg gttgaaggag cacgcggcgg cttctttcgc
     8221 gcgcgccgcg cgcttcttgg acctggctga tttcatgagc tatcgcgcta cggcggtgga
     8281 cgtgcgttcg ctgtgcacca ccgtctgtaa gtggacttat ctggggcacg aatcacggtg
     8341 ggcggacgac ttcctggtcg caattgggtt gcccgggttg gcttcggtgg gtcaggccaa
     8401 gatcggtcag cgtgttcgcc cgatgggcga acggattggc acgctcacgc cgcgcgcggc
     8461 agccgaactc ggcctgacct ccggaacggt ggtcggagtg gggatcatcg acgcgcacgc
     8521 tggtgggctc ggcctgcttg gcaccccggt ggctgggcag gttgggcaag cattggacac
     8581 ggcaacgcta tcccggcgct tggccctgat ctcggggacc tcgagttgtc acatggccgt
     8641 atcgccggag cctcgcttcg tacctggtgt atgggggccc tacttttcgg ccatggtccc
     8701 ggggttgtgg ctcacggaag gggggcaatc tgcaactggc gcgctaatcg accacgtcat
     8761 tcactcgcac gcgcgaggtg ccgagctggc ggagcgcgca ttgcgcgaag gcacgagcgt
     8821 atacacgttg ttgaatcgcg agctcgcccg gctctcaccc gagagcgatc cgcggctgac
     8881 ttcagagcta cacgtttatc cagattttca tggcaaccgt tcgccgcggg ccgacgcgac
     8941 actgcggggc atggtgagcg ggctcaagtt gtccgacagc ttggactcgc tcgcgctgct
     9001 gtacctggct acgattcaag gcatcgcgca cggtacccgc cacattctct gggctctcga
     9061 gcagcagggg tatcggatcg agactatttt ggcttgcggc ggtgacacca agaacccgct
     9121 gttcttgcgc gaacacgctg acatcaccgg ttgcacgatt gcgttacctc gcgagcctga
     9181 agccgtgttg ttgggagcgg cgatgctcgg ttcggtcgcc tgcgggcgtt acgcaacggt
     9241 ggtcgatgcg atgcgagcca tgagcgcacc gggcagctcg gtcgcgcccc gccccacgtt
     9301 tgccgcgtat cacgagcgta agcacgaggt cttccaccgc atgtatctcg accaactcgc
     9361 ctatcgcgat ctcatggaat ccgccccgca caaaatattg ccgtaagagc ctatctgggc
     9421 cttatcggtc gggggcgtat cagtcaccca ccagaatgcc gccttttggg ggaccaggct
     9481 tctttccttg gaggccctgg aaacgagtcg agggtgtgca gactcgaaga tacagtcttt
     9541 ccgttcagct cgatgtgctt gcgcgaagtc tcgccgacgg ccctcgccga tttccggtgc
     9601 agaggcgctc gctggaggga ctctaggtag attcttgcct gccgaccggc tccggtctgc
     9661 acctatgctt tggctggtac ctacagccgc tcgcctctgc actgacgaca gtgcctcacc
     9721 actctgacgt gcgattcaaa gcgattcacg ggtgcatgcc tgacgagcgt cacgcgagtc
     9781 cctcttgccg gagccgctcg aacagagttg acccgcgcta cgtagctacg ctcggaacac
     9841 gtgctcgata gaagccgcac atacaagctg cagatgccgc agatgaaggg ttcagcaact
     9901 cggtgagcgg ccgttcggtg tagttgccgg cgtgcaatta ggagcgaggg caacgctgga
     9961 ccggagtgca cattagaact gactgccacg gagccggagt ttcggctcga tcatccgtca
    10021 aagctgcctc gatggctgcg ggcagccagg tgaatccaag tgaggtcgaa agtctactcc
    10081 aggtgggttc gtttctacct gagccgattg aacgggcgat ggctcccggg ggcatcactc
    10141 cggcgggtca aggcaacgcg catcgatcag cggcgcacgt gtcatctgac tcctcgttcc
    10201 gatcaagtcc gtccgatggg agcgaagcca acagttctta tgcttggacg ggagcgcagc
    10261 cgtccgttcc cccggtgcga aacgctgtgg caagggcgcc agcctgcgtg gtcgagccgc
    10321 tatcttccgc ctttcaacgg cgaacctcct cgtccgagcc gcgagcgcga acttcgcgac
    10381 gatcgcggcg tgtgcctgcg ctttatccac gccggtacct aaactgccgg cgcgtgacgt
    10441 gcattgcgaa agccacgcgg cgtgagggaa cggccgtcgc cgtatctctg agtcctcaaa
    10501 acgggtggct ggaaaagagg ttctcgaacg aaaagctact ccactcggtg acccaagcct
    10561 gcagccagcc atccgcatcg caacagcagt ctcgaaaggg gccacggtca cgtggagaat
    10621 cgcctgcatt gagcgcacga gctctcccga cgcgctatct gctcgcaccg gagcagcatc
    10681 gcgctggcaa ggcatcgcgc tggcgcagca ccgccaggca caacatcgcc gggatagaag
    10741 caggcggccc aatgatccga gtgaacgtgc ccgagacaca atcccggttg aaccagaccg
    10801 tgaacgcaac ggtttgcctg cagaccaccc gagcggttcg aaccattctg accagttcga
    10861 cgcgcctcac gcttggtaca tcaagcacgt tccgccccag aaacgccgcc agaatgcggt
    10921 caggcgcgcc gcccggaaaa gacgcgccga cacaacgctg accgaaacga cgccgaagaa
    10981 acccagcagc ctctcccgct agcgagcaaa tcgatgaacc acgaacctcc acactctgca
    11041 ctggcgaaag ctctttattt gtttcaaagt gatgttcccc aagctaagct gtctggccta
    11101 gcgtcgccgc tctcctccac gagcgggctt ctgagaaagg cttcccgtat gcctaagttt
    11161 gccctggttg tctgtgtttc ggctctggcg ttcgctgcag gttgcagttc ggactcgggc
    11221 gacggggcga gttttgccgg cggtggcatc ccggggctgc cccccaacca gatcccgggc
    11281 gctggaactg gttccacagg cgcgggtggt ggaaacattc caacccccgg gggtccgctg
    11341 tcgggcgggc aaatcgccat ctcaccgcag caggtccaaa ccatcaagca gcagtcctgt
    11401 gccggctcct ccgtcgaggg ggaaacggtg ccgtccgtgc tgcagttggt cgtggacgtg
    11461 agcagctcga tgaacgacca ggcgcccggc tccaatcgga gcaagtggga ggttacccga
    11521 gatgcgctca tcaacgcgat cccgggccgc agtggaaacg ccggcttgag cccgtctttg
    11581 ggggtggggt tgctgtttta cccgaaccgc cacgtcgacg tgagtacgac cccggtcgcg
    11641 actgaccagt gcgtcaacac ctcggccaac atcgtgccgg cgctgttggg accggccaac
    11701 gcacagcacc gcacgctgat tcggtcctcc ttcgagcagg ttcagctgca atcgagcacg
    11761 cccaccctcg atgcgtaccg ctatgcgttc cagaacagca tcaccaagtc gacgttgacc
    11821 ggccaaaagt acatgctgct catcacggac ggtacgccga ccttggcgct cgggtgcatg
    11881 aacccttcgg gcggcctgag tgacgtgagt gcggccgaca tcgaagcgat cgtgagtgag
    11941 gtgaaggccg cgcgcgccgc cggaatcaag accttcttga ttgggtcacc gggtagcgag
    12001 cgcaaccgga attggctctc ccgggccgcc atcgagggcg gtacggcacc agcagggtgt
    12061 agggcggatg gtccgaacta ctgccacatg gatatgacca cggcgccgga cttcggaaag
    12121 gcgcttcaag atgggctggg caaggtggtt ggccagattg ctccctgcaa ctacagtttc
    12181 cccccgccgc cgaacggtga gacgttggat tccaataaga tcaacgtcat cgtcacctcg
    12241 ggggggcaga gccaactcgt cgtccgcaac gacgttggca actgcaccga gggttgggag
    12301 ctcaccgatt cgcaagagat tcggctctgc cccagcacct gcaatgccat acaacaggac
    12361 gcctctgcca cggtagacgt ggtgtttggc tgcgcgagct tgtccgagcc gccgcgctga
    12421 gcgcgcaaaa aaggctgcag taacgcagcg gcaactcgag agcgcgttcc gtcaggagcg
    12481 cgctcttttg ctttgctgac ccgctcgttg gcgcgcccta tttgcggcgc aggcgatcga
    12541 tcccgacggc ggcgacgatg attgcgcccg tgaagatctc ctgaacccaa ttcggtacac
    12601 ccacgtgggt gcaaccagtc ttgatttccg tcatcagcaa ggccccgacc agtgttccca
    12661 cgatcgagcc ttggccgccc gacagcgacg ctccaccgat gacgacggcc gcgatgacct
    12721 cgagttcgag accaatcgag tcagttggat ctccgaccgt caaagtcgcg tactccaaca
    12781 cacctgctag cccggcgagc agccctgcca gcccatagac gagaactctg actctggcca
    12841 ccggtacacc gcacaaccgt gcagtttgct cgttcgagcc gatggccacg acatgtcgtc
    12901 caaagacggt ggagttcaag gcgaccgcgg cgagtgccgc actcgccgct aggaaccaca
    12961 cccccggtgg caaccccacg ccatccgtgg cggacatcca agtatcgatt ccgaggggat
    13021 tagcgtcgat cttttgctcc ccggagatgc ctttggcgag ccctctcagc aagctcatcg
    13081 tgccgagtgt cacgatgaag gcgggaatgc gtaagccagc gatgaacgcg ccgttcaccg
    13141 ctccgctcag cgccggcacg agcgcggcaa gcgcagatgc gctgagtggc ccatatccgc
    13201 tcttcagtaa ccacgcgagt gcaaccgtgg tgagcgcaac cgccgaaccc accgagagat
    13261 cgatgccacc gagaatgatt acgtaggtca tgcctaccgc ggtgatggct accactaccg
    13321 tttggcgcgt catggtgagt aggttggcgg cgccgaggaa cgtgtctggc gacagcaccg
    13381 agaacagcac gtacagcgct aggagcgaag cgagaggccc aaaccaaggg cgccgccaga
    13441 tcgtggtcac gcgcccgttg cctcctgcag cacgcccgcc tcggtgaggc ttcggatcgg
    13501 gcggacgggg ccgagctgac cgcggtgcat cacgcacaga tcgtcacaca cggagaacaa
    13561 ctccggtagc tgactcgaaa ccatcaaaat cccttttccc cgactcgaga gttcgtcgat
    13621 ccaaccatgt atttccgcgc gactcctgac gtcgatgcct cgggtgggct cgtccaacaa
    13681 gagaacatcg acgtcgtgat agagcaggcg cgcgagcgca atcttctgtt gattgccgcc
    13741 ggacagctcc tggatactct gatccggtcc tcgacaacgc acgcccaagc gctcgatcca
    13801 agcactacag atcttgcgct cctcggctcg agacagaaga ccatgtttct gcgtgggggc
    13861 gagccgcgag agcgtcagat tctcccggat cgagagctcg agggccagcc cttcgagttt
    13921 tcggtcctca ctgagcaacc ccacactttg ttgcagtcga acggcggggc ttgccgcgcc
    13981 gggccaggcc cccaccgtga tttcaccgcg aagcacggga gccaagccga atatggcccg
    14041 cagcagttcg gtccgcccgg atccgacgag cccggcgatc ccgagcacct gccccgcgtg
    14101 gagttcgagg ctcgccgata cgggcagcgc cgtgccagcg agttcttcga ggcggagcaa
    14161 cacacgacct ttcggtcgag cctcgcggac gcgctcggca attgcttgcc cggccatcgc
    14221 gctgaccagg tcgcgattgg tgacctccgc tattcgaccc cgagccaccg ttcttccgtc
    14281 gcggagcacg gtatattcat ccgcgatgcg ccgcacttcc tccaggaagt gggagatata
    14341 caagatcgat agtccccgtt cgcgaagcct ggccaaggcc gcgaagagcc gctctgtcgc
    14401 gtcgccgctc aaggtgctgg ttggctcgtc gaggatcagt aacttcgtcg attcggcgca
    14461 gagtgcgcgc gcgatctcca cgagctgccg gtcgctgggc gccaaatcgg cgagcagcgt
    14521 gtcgggcccg atgcgcgagc ggctcgggac gacccaagtc agggcacgct ccgcacgtgt
    14581 tcgcatctct ctcctcgaca gccaacccca tcgcgatggc tcgaccccca gcacgatgtt
    14641 ctcggccacc gtgaggtgcg tgcagaccga cagctcttgg tagacgatgg ctaccccgag
    14701 ccctcgagcg ctcagcggat cgcgcggcga gtacgctttg ccttcgagta gcatctggcc
    14761 ctcgtcaggc tgcatggcgc cggccaagat tcgcatcaac gtgctcttgc cagcgccatt
    14821 ttcgccgagc acggcatgaa tcgagccggg agcgacctcg agagctacat cactgagcgc
    14881 gcgtgtcgca ccaaacgact ttgcaatccc gcgcgcggat aagcgcattt ctagagacta
    14941 tcctagattt cgcgcgcggg ccgcacgaac ttggaggtgt cgaagcgaga tcgcgtcagc
    15001 ttggccgctg gctccgatac gttcagggcg acaaccattt cgcgaggtcg ggctttacca
    15061 actcctggat ctcggcactc tccaggttag ccttgtcgac gatttgggcc ccggtatcgg
    15121 tgcgaggggc tacgctctgg cctttgatgt gggccaccag agacttcacc gcgaggtaac
    15181 ccatgttgaa ggggttctgc acgaccagcg cgtcaatcgc gccgctgttc aaagccttca
    15241 atagcttttc cgaggcatcg aatcccacca tgtggacctt gccgctcagc ttggcctttt
    15301 cgagcgccag aagcatgccg aaggtcgtgg actcgttggg ggcgaatacg ccttgaacgc
    15361 cgcctttgtc cgcggctttg gccaacaaca ggctctcgga cgccgagaac gcggactcgg
    15421 tggtggcgcc gccgtactga ttgtcactca cgacctcgat tcccggttta cccttcacgg
    15481 cctccaggaa tccttcttcg cgttcgtggg tgctcgcgga gccttcttgg tatctcagga
    15541 ctaccacctt gcccgggcca ccctgcagga gctgcagcat gcgttgtcct gcgaggcgac
    15601 ccgccgcgcg attgtcagtg gccacgaagc tgacggtgtt tccgccggag agggccgagt
    15661 cgaagatcac caccgggatc ttttgttgag tcgagcggac tacgctgctc acgagtgctt
    15721 tgtcattgag tggagccagc acgattccgg agactttctg ggccgtgaat gactccacga
    15781 gatcgacttg gctcttgagg tcgtcctctt tgagcggacc tttccatacg atgtccacgt
    15841 ccagctcgcg cgcagctttg accgcacccg cgtgcacact cttccagaat tcgtgagtag
    15901 tgcctttggg aatgacggct aacttgagac ggcccgaggc ggccttatcg gtcgtctgtt
    15961 ggccttgtgt cttttggcag ctggaaagcc agattgcgct caacccgata acctgtttca
    16021 ggaactcgcg tcgcacggtc acctcaccct gtttggcgaa agcacgcacc gagagcagct
    16081 ctgaggtaac tttcgctgaa aagagcggcc ggctctgttt gacccttcct ctcgagcctt
    16141 gttgaggctt ccaacgcgcc ctccgctcga gctcgccgcc actcgtccga ggcaggttat
    16201 cagcaaatgc caccaggtag ccaaatcaaa cgcccggtcg gcgtattcgg cgcggagctg
    16261 ctggaaagac cagctgtcga agctgacctc tagcctagtg ccgacaattc gaggttgaag
    16321 cgtaccccag agccggagca taagacctca atggtgcgac gttttgcccg gctaggttcc
    16381 gcgccccttt ttgcgctgtg ctctaagacc ggcgccgccc cgtgcgccaa acccggtgct
    16441 gcactctgcg ctgcgatcac gctggcggcg gctttatctg cgccgcacga ggcgcacgcc
    16501 caggcgttgc gggaaccagg aaaagtcgca atttcgatcg agcgcgcgtt cgggatcaac
    16561 tactcccaca ccgagatcga taagcctctg cacgccgaac gaagcgacac gaccttgggc
    16621 ttcggtttcc agcccgggct ttctccgctg cactgggccc gcttcggtgt ggatgtgttc
    16681 atcaacgagc attggagcgt cggcggttcg gtcggcttct tcaaagtaag cggcgacacc
    16741 gacgcttcgg gcttgttgct ggccccgcgc gtgggctacg cactgccgct cggcaagttg
    16801 gtcacgttct ggcctcgcgc cggcttttct tacgtaaaac ctggagacgc acacctcatt
    16861 ggcctgagct tggaaggcat gttcgtgatt tcagcgaatc ctgcttgggg tgtgctcgtc
    16921 ggccccacat tggatgtagg aatcagcggc aagtcttcgg gcggggattg gtcggagctc
    16981 gccgtcggaa ttccgacgct cggcttggta gcgacgttct gagccgcgcg ctgtaaattg
    17041 agggcatgcc gcgactcaac ttcacgccga acttggagcg ccacttgggt acccgtagcc
    17101 tcgagttgcc cgcaaattgt ggcgacactc tgcgcgaggt gttcgatgcc gcctttgctc
    17161 ggttgcctct cgccagagct tatatcttgg acgatcaagg cggagtccgt cggcacgtcg
    17221 ccgtcttcgt cgacgggcag ctggtcagtg agcgccagcg gctcgccgcg tccgtcgctt
    17281 cggacgccga aatctggatc atgcaagctc tgtccggtgg atgagcccag accgctcagt
    17341 cgccgcgccg tatcccagga aggcccatga aaatcccgca actgttcgtc gccacccgca
    17401 aaggcctgtt cacgatcgcc aagcatctcg gagcttggga catcgtagat gtatcgttcg
    17461 agggggacaa tgtgtccatg gttctgccgg acacgcgaga cggcgccgta tacgtggcga
    17521 tccagcacgg gcacttcggc agcaagctgc accggtccga tgacgacggg aagacttgga
    17581 aggagctgac cgcccctcaa tatccgccca aaccagagtc cgaagtcgat aaggatccga
    17641 ttcggggtga agacgttgcc tggagcttgc agctgatttg gtcgctagag gccgccggtg
    17701 atcaacgcat ctttgccggt acggtgccgg gcggcttatt ctactcggac gatcgagggg
    17761 agagcttcaa cctgtgtgaa tcgctgtggc tccggcccga gcgcaggcgt tggttcggtg
    17821 gcggcttgga cgcacccggg atccactcga ttgtggtgga ccccgccgac ggaaatcacg
    17881 tgctgattgc ggtctcgtgc ggtgggatct ggcggacgcg ggacggtgga ggttcgttca
    17941 cgctcgaggg caaaggcatg cacgcgagct acatgccgcc cgaacgacga caagacctgg
    18001 atatccagga tccacatcgc ttggtgcagt gtgcatcgaa gcccgaggtg ctctgggttc
    18061 agcatcacaa cggcatcttc cgctcgacgg actacgcgga aagcttcgtc gagatcagcg
    18121 gcgcgaagcc gagctccttt ggattcaccg tcgcggcgca tccaaaagac cctgaggtcg
    18181 cttggttcgt tccggccgct agagacgacc gacgcatccc cgtcgatcgt cgggtggcgg
    18241 taatgcgaac ttcggacggg ggccgcagtt tcgaaccgct gacgcgcggt ttgcccgatc
    18301 agttcgctta cgacctagtg taccgccacg cactggacgt cgatgcgacg ggcgcgctct
    18361 tggcgtttgg gagtacgacg gggaacctct ggatcagcga gaacggcggg gacgcgttcg
    18421 caaccgtgtc tcaaaatctg ccccctattt acgcagtgcg ctttcagcac cctcacgcgg
    18481 cgcccgccct ggaacgcgtt caagcggcgt gagctttcgt gatcgacatt cccccgatct
    18541 ggcgagtggt acggccgctc gcggagctca tcgactcccc ggaagcagcc gggcgggctg
    18601 ctgatctcca gcctgctcgt actaagccgg cggccgcggc tccacctcgc atccagctcg
    18661 tgatctcgcc gggcgccggc ttcgggcgtg gagatcacga gacgacgcag ctgtgcctgc
    18721 aagcggttca ttggttccgc ccgccggggg ccggcggttg gcggatgctc gatttcggga
    18781 gcggatcagg catcttggcg gtggcagccg ccaagctagg agcgagcgtc gaggcggtgg
    18841 agatcgacga tgccgcgatt gcacacgcgg agcggaatgc gtgtttgaat ggcgtgcagg
    18901 ggaggattct ttactcgaag agcctggaac cgttgagtgg cgtcttcgag ctcgtggtcg
    18961 gaaacatctt gttggaggtt ctgctcgcgc acgccgaagc gctggtcgct cgcgtggctc
    19021 cagggggaag gctcgtgctc tcgggcttgg tcgctacgga cgtaccggcg gtgatcgcgc
    19081 ggtacgcccc gctgctcgac cagagccgac ctgacgtgta cgagcgcgcc ccctggcgct
    19141 tgctcacttg gcgacgtggg ccaagcgacg cgggctgagg ggcgcgccgt ccaggggagg
    19201 acgaaacgcg aggacaaaaa acaaccagcc ccgcccggtg tctccgcggc caacctgtgc
    19261 cgaggagacc cttgcggggc cgttgatcaa gcgcttcgcg tcgcgaccga gggcgatcag
    19321 ccgccagaga tgctcacgat acagacgtca aaggctctca ccgcgtcttt ggtgcccgga
    19381 atcgagatct gaattgcttg gataggctca cccgcgtagg cgactgccgt cgcggtgtcg
    19441 taacacttgc tcttgaagct gctcactggc agcgaaaggc ccgtgccgtt ggccgcagtt
    19501 acatccgccg cggcgagctc gtaacagaac tcggcggcag tgccggccgg ttgcagaccc
    19561 acgcgcatgc ccgcggtggc acccgcgagt tggaccttca ccgtgcccgt gagcgccacg
    19621 gtatccggcg tgccgccggc cttggcttga ttgatactcc agccgatgcc cgcgccatcg
    19681 tcgtacgtgg caggtacgct gccggcggcg caaaggacgg cgccggggaa gcaactctgc
    19741 gtttggctac tgcccggggt gatggtggcc gtgccgtact tgtacgtgta ggcatagccc
    19801 atccaggtgc cgctcgtgcc gtagccttcc atgaagatgg tgctggcagc tttgagcgtg
    19861 ccgctgtacg tcgcgcagct gctcgtcgcg gtgccgcccg cgcccgtggc ggtggttccg
    19921 ccggcacccg tggcggtggt tccgccgttg gccacgacgg tggctccacc gttggccgcg
    19981 gtggttccgc cggccgcaga ggttgccccg cccgttgcgg ccccgcctgt cgcggaggtt
    20041 gcaccgcctt gagcactagt cacgccacct tgggcgctcg tgaaaccacc gaagccactt
    20101 ccgctgaatc cggttgctcc gctggtgttg ccattggctc cgccagtggc agctggcgaa
    20161 ctgcctccgg tgccgtcttt gtgttcgact ggatcggagg acgccgagca cgccaccgtc
    20221 atcaaggtga cggccgcgaa gcgcagcgaa ggccctgcga aagcgaagcg tgatgcggca
    20281 cgagaagaac ggttggatga agtcagatgc atatcgatct ctccgaggag cgtcgaggcg
    20341 ctaaagaacc agcggcgttg caggcatacg cgccaacgaa gtttcgtcaa gtgaccgatt
    20401 cgatcgagtg ctcggctggc tgagtgaccc ctcccgagag caacccgttc ggagaaacct
    20461 gcgagggggg acctgccggc agaacaaccg gttctacacg taactttgct ggagacttac
    20521 tttgctggac acgcctgtct ggcaactggc ccaggaaacg tacgagaggg atcgcgatct
    20581 gtaaaggagc tttgtcgcct aggttgcgac gcagttgcga gtcctcagct ggtttccaat
    20641 ttcccaatcc gaatcggtct ttcccaatcc gaatctgcac gactctactt cccgcgctcc
    20701 tacctccgcg ggcctaactt gtggcccatc gctagccttg cgccgcgtgt ccgcggccat
    20761 acggcgaaga gcatgatcct gcgcaggcgc tctctcccca ttccgcacta gaccatcgag
    20821 gctttgagtg ccgagcagaa gtttttgcct gggctcgatg ccactgaacg acgactcaag
    20881 gcgaagggcc ggtagagacg ctgaaaagcc caagcaccgt cgcgggtgct cgagcccaag
    20941 ctgagccgat tcgcgcggtc atagccacat cgtcgcagac ggcgcatggc gcgccaaagc
    21001 gcgacactcg aatatcccga agattacatc caaaatctgc ttgcgcttga tattggccgt
    21061 gaacagttga accgtgcgtc aggcaactcg atgcgattcg aaattaccat tcgttgcgtg
    21121 tttcttagca acctgctcgc ccttgccagt acgaacctcg cgcgggctgc tgatccaacg
    21181 ccagctccac ccacgggggc cacgccagcc gcccctcctg gagcacctgc gccagtaccc
    21241 accagcccaa gcgcaccgtc gcgtgccgag ttccccaatg cggcgcaagc ccccggtgcg
    21301 tcgactttgc cagcaagtcc gagctcgtcg ccaccacccg gcactgctcc atctggcgcc
    21361 gctcaggcag gtactgctcc gccaggtaca gcgaccggct cgaccgtgct acctgcagag
    21421 cggcccctgg agcctgggac tggttccggc acgagtcaga ccacccccgc atcgccggtg
    21481 actccggtcg ttacgccgga acccccgagc gccacgtatg aagaggaaga agaccccggt
    21541 cgtggaatga gggcgggcgc tcttccagag ctcgaggaga ttcgcttggc caaaccagaa
    21601 ggcgtggcct tcggcctgga tggttatggt gggattgcgg tgttgagccg ccaaagcacc
    21661 ggagcctctc acgcctacct cggtggcctg gcgcgggtgc gggtcggtta tttgcagttc
    21721 ggagggagct tcgagcaatc ggacgtagtc gataacgaat ggagaacgat tggcggcttc
    21781 gtcggcgcgt tcttcccttt ccataactgg gtggacttcg acgcggccgt gggtttcgcg
    21841 tcgcggcgac acgccagcag cgacgggcgg tatggtccgg atgggtacaa catatcgatg
    21901 ccggccatga cgttgcgatt cggtgtgtcc gatcgcacat cggagagcct gtccggcgtg
    21961 cgcgtcggag cgtatctcat gaccacgatc gatctgaagc aacgccgagt gccctggtcc
    22021 ttcagcatcg agaactcttc gggtgaggag gtggtcacga ccggctcgag tcgagtgggt
    22081 ggcttctcca tcggtggggc gttcgcgctc ggattcgata tcgtgcccgg agctcacgac
    22141 gcgccgcccg tgcccccgaa gagcgctgaa ccggggcgtt gaccaaagcg ctcagcccgg
    22201 aatcagctct gcgcgcgcga agcgggcgtg cagagctacc agcggcaggg tcaggcgtcc
    22261 cacccgggcc aacacgtagc gttccagaca cacggtcaag ccattcacgc gcgttgattc
    22321 gcgccaatag tgcgccccac gcgcccagaa ccccaagatt tcggcatgat agtcgttccg
    22381 aaccagctga cccgcttccg agaaatagaa gcgctgctgg cgacaatgcg acggcacgtc
    22441 ttcggggagc tccacttcca agacgtccag cgttcgcccg cggccacgct ctcgataact
    22501 gctcttgagg gtcgcctctc gcagcgtgaa gggcagcgcg tgatagtgcg tgagcgcgta
    22561 accaaagaaa tagagcgcgt ccaacgcgga ccagcgacga tacttggaga gcccacggaa
    22621 gcttcgtcga tgcagctccg agcggctcga gacccccgcc ggtccttcga tgcgtaccgc
    22681 gccattctcg aaaatgccga cgctgttcgg tgcggggtag cccacgaaac gcgcgcagcg
    22741 ctggtgcggc atcacgtcga agtactccgg catgacgaag gttcgaccaa gccccttcaa
    22801 tcgcggaacg agcccggtca acccattctc gactggatgt agacggatgg tctgcgcgtc
    22861 gttccaagcc gtggcacctc cggcgtactc gcaggctcgg gcgagcacct gggcagcgtc
    22921 gggtgaccag ctcgagtgca gcgacatacc tgacgtgggt ggtaccagag tgggcggccg
    22981 gcgttgcgtt atttattgcg gcgtgcgcgg tttcgcgagg gcgacgaggg gaacgaggct
    23041 gcgcttccgt ggactctgga cgcgcggcgc gcccgttgcc ttcggggccg ccgattcgaa
    23101 tgcaggagaa tcgatcattg ttcgaccgct cgggtcacct gctgattccc gcctcagttg
    23161 gactcgtgat tcgggtcgag gacgacgtgg ggctcggcag aggatgcggg gagccccgga
    23221 gactctaaac tgctggagca tgcgccaccc gatgctgctg ctcttgctcg catacttggc
    23281 gttcgtctcg ctcgggttac cggatacggc gctgggggtg gcgtggccgt cgttgcgtcg
    23341 gcacttcgat ctgcctgctt cggcaatcgc gacggtgctc accgccggca tggccggcta
    23401 cttcacctct ggtttgttgg caggcaaatt gatgcaggcg ttgggcgtgg gcggagtgct
    23461 cgcgttgagc tgtggattcg taacggcggc gctcacggtg tatgcgctcg ctccggcgtg
    23521 gtggttcttc ttccccggcg ggctgctgat ggggattggt tcgggcgcga tcgatgcggg
    23581 tctgaacggc tatgcagcgc ggcacttttc cgtcaaacac gtgaactggt tacacgcctg
    23641 ttgggggatt ggggcgagtg tcgggccggc tttgatgacg ggcgcgattg cgcgaggtaa
    23701 cggatatacg gcgggttacg ggctactggc gctggccttg ggcggaatga cggtcgcgtt
    23761 tgcttggacg caccgacttt ggaatgagcc tgctggggag gtacgcaccg cgcggtccac
    23821 agaggcccaa gcggacgctg ccgtccaagc tcacgccgaa gtgagtgcag cgggcgacgg
    23881 cccgcgcgtg acgatgttcg cggcgctgcg gagcggcccg gtgtggctgc acatcgttgc
    23941 cttctttctt tataccggct tcgaagcctc gaccggtcaa tggtgcttca cgctgctcac
    24001 agcgggccgc ggactttcgg tggagcgagc gggattttgg acgtcgtgtt actgggggag
    24061 cctcacgctg ggtcgcgtgt tgctcggctt cgtcgtggat cgtttcgggc ccgatcgatt
    24121 gctgcgctac gcaaccacga gcgccgcgct gggggcggcc gcgtgggcgc tagatccagg
    24181 gctcgtcggg aggttgggac tgttgttggt gggtctgagc ttggctccaa ttttcccgac
    24241 cttgatggcg cgcactcccg ctcggctcgg c
//
LOCUS       DASTKU010000585        14820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1005139, whole genome shotgun sequence.
ACCESSION   DASTKU010000585 DASTKU010000000
VERSION     DASTKU010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14820
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005139"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1181
                     /locus_tag="VFQ61_20480"
     CDS             186..1181
                     /locus_tag="VFQ61_20480"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9956890.1"
                     /translation="MRILFIGGTGIISSACSDLALARGLDLYLLNRGKSELRPAPRGA
                     KLLSADISDRHAVENALGSLTFDAVVNFVVFTEDQIQRDIELFGKRTAQYVFISSASA
                     YQTPPTRLPVTESTPLHNPFWQYSRNKIACEERLLRAHREQGFPATVVRPSHTYDRTL
                     WPMHGGYTAVDRMRRGRPVVVHGDGTSLWTLTHHRDFAAAFVPLLGHDGAIGEAFHIT
                     SDEWLTWNQIHEIVARAAGTRAELVHVPSEVIATHDPEWGASLLGDKTHSMVFDNTKI
                     KRLVPDYRASIPFSQGVHEIVGFYDQNPQHRQIDRELDRLMDKLIDSQVRERRAL"
     gene            complement(1257..1988)
                     /locus_tag="VFQ61_20485"
     CDS             complement(1257..1988)
                     /locus_tag="VFQ61_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956891.1"
                     /translation="MFGVFACVALTLAAGCASSAPSATQSTASAATPPAPTTSSGLPA
                     PPASSNQNPPAASVDSTVTGTDSAGVQPPPPMAEPPETVSPEPTPTPVPKRPRVEVLS
                     APDLAYVVDYANSGAREAALGACKDKEPDGAAISACLEKARDQFLPDVLRFKKTGSDY
                     SLAIYKRSGSTLRELYVGGVVLTEAPDSRVKVTFTGRERGVRPLFRGRKEGWIGVPTD
                     YSLEIEDEGLGKLTYTAKIGLVSSP"
     gene            2238..4085
                     /locus_tag="VFQ61_20490"
     CDS             2238..4085
                     /locus_tag="VFQ61_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956892.1"
                     /translation="MKRSSSLQQLIVAWAAPYLVVAAPTLTLCPRPALAQGESRAEPT
                     TSEPLKIHVALSSPALDAERVRREVAGELRREVVLASGDEATLTITESAANAASPSRA
                     QRVGATLQFRYVTREGRVLTRAMDLPRDPKKALQMIALLSGNLARDQTSDLIGALHDQ
                     RTAALSPSAAVEAEPARTDATPAPPEPAANDSQAPPPSAHEQGGNKQHEGARRDEGST
                     AKSGAAAHEESKPAQSKPTAPRSYAPLNLSLWHPLSLNPNSHEQGYRFELGLAYSRIG
                     ALGGAAFSAGVLRVDGPVAGAAFSSLWLSSAGPLRGLIASGIFVQNHGDSEGAQFSGV
                     VAHHAEGEFTGAQFAGAVSLAGDLVGIQASGAYSQSQRLRGAQFAGAATHTKEASHGI
                     VGAGAVTWTHSLKGAAFAGALSVSEDVDGLVAAGAATVARDVQGLAVAGGVNVQRDVS
                     GVALATVNVQRRVRGLQIGVVNVAERVDGAAIGLINLAGNGRVSALGWVDQDAAYGGV
                     RFISGMSYTELAFGHEFDSKRYVTDAGLGVHVPLGQRITLSVGGAFSETRTRWDEDQD
                     HAEAIGRGSVGLRLTPMVELFAGAGLRYGVLGDTKEEFRPEGRLGVAIF"
     gene            4235..4885
                     /locus_tag="VFQ61_20495"
     CDS             4235..4885
                     /locus_tag="VFQ61_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9956893.1"
                     /translation="MLPLGFHPSLVSSPFYGTRLTPLAFASNATLGSSRVDAELALIE
                     RIADAEPRAVGEAYDAHHGAVRAFAERLVGEAAAAEDLVHEVFLALPSAAQKFEGRSS
                     LRTFLISIAVNRAHHFVRKASRRRKALDRLEKEPSPGNSTPEHLTERRQLAHALSRAL
                     DTLPVDQRVAFVLCEVEDRSSREAAEIMSVPEGTVRTRVFHAKRKLRIELEREGFR"
     gene            4882..5688
                     /locus_tag="VFQ61_20500"
     CDS             4882..5688
                     /locus_tag="VFQ61_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956894.1"
                     /translation="MNHDLLKAATRALREETERPDQDGSETRARLMASLHQTRVRKRT
                     RLAFALPLAASFVAATALGRLDQRIPQLVRSVAHSLGWSANTGDTAPPPSPRKNVGST
                     GSKLSPTRTTPTRNLAPVESVVPPTEPVATPLDAPTSPQAPEIPPSASSGAAPRAGGN
                     PPPQLPAPRASATEQDAGLTRELDLYRTAHRAHFEQHDYVRALQGWDRYLRLMPNGRF
                     APEARYNRGVCLLRLGREAEAQQALAPFASGQYGEYRRSEAQAVIEALQR"
     gene            5743..6789
                     /locus_tag="VFQ61_20505"
     CDS             5743..6789
                     /locus_tag="VFQ61_20505"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9956895.1"
                     /translation="MRSRENLSALVLTCGLWFGCARSDAWDTQVEHDVVLPEFPTESC
                     GPQVLQVTTEHPKVLLLLDRSASMQDPPETEDPDVVGTTSKWDLVLPALDEVIAQTDT
                     ALDWGMKSFPEGDGTAECAAESVTSRIDVSVASSNASRVVEQIGLTTPQGSGTPTGDA
                     VRSALAYLLTLPAGPPKFLILATDGEPSCAGTTKGQETARPYAIEALQQALSSGVPTF
                     VVGVATNKESATNALNAMAVAGGQPRAVTRDTDPRYYLGNTQDELLSALSEITADLTG
                     SCSIVLHRPPESPELARLRISGAEVPRDLTHQEGWDFVGTERIQTFGAACDRTRKAAP
                     GSLEIVYGCVQALP"
     gene            6814..8475
                     /locus_tag="VFQ61_20510"
     CDS             6814..8475
                     /locus_tag="VFQ61_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HET9956896.1"
                     /translation="MKERLQGFLLVMAVGAAVAIGAKYLSVPYNVALVVVGLFLVFVD
                     VLPHASMDPQVILVLFLPLLVFEAALFSDAKSLRRASRPILALAIPGVVISLIGTAAV
                     AAFALALPFSAALLLGAVLSITDTISVVLAFRSVRVPHRLHAIMEGESLFNDGTALVL
                     VSVTSAILLHGVFDPVSTLRTLLVAILGGVAVGSMIGALGAAVLRRTPDHLTAILASI
                     VLVLGTSALAEEVHASPVIAVVVAGLFVGNAARSALEPSRVLALQGFWEVTGFVINVL
                     LFLRVGMQLDPKILITEAPSIALALVALHLGRAVAVYGCFLGLQWFANENVPRNWQHI
                     MVVGNIKGALSMAAVLALPHNVPYRHRLIAIVFGATLVTMVTQALPFRRFLHWFKVVE
                     GGNEARMDAARASLIAARRGQLELDDLLESGLLSRRSHAERRAQFQRRIIAAEAILHE
                     VEGERPRDRLVDTALLYAQKASVLDAARRGLVPEPIANAEVERIDSSILDLELNHDHD
                     EEHGPASSAAPAHGAKPQPREGLPPERRPAFYSLSPDRRHEKDPP"
     gene            8472..9146
                     /locus_tag="VFQ61_20515"
     CDS             8472..9146
                     /locus_tag="VFQ61_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET9956897.1"
                     /translation="MMRVFIAGAGQAGVSVAKHLSNLGHEVSVVDRDPLITRRIFEQH
                     GIAAITGDATDAALLQQAEIGRADVAVSMLARDADNLAVALLAREFGARRVMVRMRDV
                     AYRRPYEHAGVDRILSETEILVGSLAISIEHEAVGHAMILGDGNSVAFDLTINHAARV
                     VGRTVSELAQSADFPRSCVFAAIFDENGMTSPRGNTQIRGGQQVLLVSHREEMSRVVD
                     FFMSRA"
     gene            9832..11505
                     /locus_tag="VFQ61_20520"
     CDS             9832..11505
                     /locus_tag="VFQ61_20520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956898.1"
                     /translation="MRSLSSSLFVGAVLVFAASCGSDKNDPPPSSGTGGASAGATSSG
                     GASSGGMASTTGATAGGGATSTAGAATAGGRTNVGGSSATGGTAGAGGKGGQGAFGCP
                     AEKPAAGGECTRMANVACTYDDGGCLCVDGAWSCYSDADCPTAAPADAATCTLGGMQC
                     AFDNVNCTCSTTNGWTCESACPSAPPAADAACRRAVNRTCRYAEGALVQGFGTPDTTC
                     ACNESKFSCFSQDDCPAAAPENTSACTMNTLSCEYDDRQCTCTNGEWNCVTDCPEAEP
                     SDGAVCMRPENATCRYAEKALLQGFGGKADSTCACADGKFSCFGQEDCPAAAPSSGGT
                     CEFASLACSYTGAQCSCSNDNTWRCTTDCPAAPPAQDAACDRPETSVCRYNDGALAQG
                     GFGAAEALCACRENKFDCLTKADCPATAPANQAECPDLTNLSCSYTDQTCTCGMNGWT
                     CQSECPAKAPAAGAACSRPVTSACLYVAGSLVESGTDPESTCVCASNAFVCYTDADCP
                     AAQPESNAACEKPGLECAFGDQNCRCRTSTNAWSCNAAPPPMGAGGAAN"
     gene            11642..12883
                     /gene="lysA"
                     /locus_tag="VFQ61_20525"
     CDS             11642..12883
                     /gene="lysA"
                     /locus_tag="VFQ61_20525"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET9956899.1"
                     /translation="MPRTDSRRRELLTRLAAQYGTPLYVYDAQVIRAQFAKLRRFDVV
                     RFAQKACPNTHVLRLLKGEGAMVDCVSAGELERAVHAGFSGGSEPAEIVFTADVLPRE
                     VSERVVELGVPVNAGSEDMLEQLGRVSPGHKVWLRINPGFGHGHSKKTSTGGENSKHG
                     IWYANLQKALVHIDRYGLDLVGLHTHIGSGADIEHLGRVCDAMVEQVRNLGRDIRAIS
                     GGGGLSIPYRPNDPQVDTDAYFKRWDAARKTIEGFLGHPVHLELEPGRFLTAAAGVLV
                     AQVMASKTAGSNHYTLVDAGFNDLVRPAMYGAWHEIEVLPASDRKDGELLPTIVGGPL
                     CESGDMFTQSKDGDVLPRNLPRSEVGDLLVFHDAGAYGASMSSNYNSRPLAAEVLIDG
                     DDHRLIRRRQSIQDLLALEAT"
     gene            complement(12922..13914)
                     /locus_tag="VFQ61_20530"
     CDS             complement(12922..13914)
                     /locus_tag="VFQ61_20530"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HET9956900.1"
                     /translation="MRIALEYSTLLGSLNLFESLAESELEQLSSRLELVEIPEGALIF
                     AQGDKGGRLYIIKEGLVDISHGEGPHRVRLATLAPGQYFGELSLFDGEPRSAAATASR
                     ACKLLALDREDFVEFTTNHPEATLQIAGELAARLRQMNAAFSGQVTRNVLEEQEEKLT
                     LGERVADRVAAFGGSWPFIGVFALGMAVWMSFNAFSGHAFDVYPFILLNLILSTLAAI
                     QAPVIMMSQNRQAAKDKLLAANDYEVNLKSELGIQALIKGQTEMLARIALLEKRLHAP
                     SRRPFPSPGGDAQSSQAHSGVRSSGTSSSPSPSSHSSPTTPSGSKSGADRDPPT"
     gene            complement(13967..>14820)
                     /locus_tag="VFQ61_20535"
     CDS             complement(13967..>14820)
                     /locus_tag="VFQ61_20535"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9956901.1"
                     /translation="GALVLTLLLIATLIGKKTGTIWDVPLELTWVSIVSHALLLQDFY
                     ATGSINYVFWSIAVEWQIYFLFPLLVLGFRRLGSLQVVGLALLIGYALRIGFAHTRIA
                     RANPHFVGMFSLGMLAAYVAQADREPFKRWRYGFPWAAVGSVAFVTAVALAMYWGFRS
                     AIHSFYWLDMPVGIFATCLLVLTSRLPQSRLRSVFAFKPLAFVGTFSYSVYLLHAPLL
                     QLMWQYVLSPLGLAPEAMFSVLLSFGLLAVLGCAYLFFLVLEKPFMSSASGSGHAAGP
                     PIPKAAS"
BASE COUNT         2649 a         4909 c         4769 g         2493 t
ORIGIN      
        1 aaggaacccc aggagcacca gacagatcgc atcgcgcgga ttgtaagtga cagactcacc
       61 tttacctcag ctttgccggc gctcggtagg cacaaaagct cgatttggca gacgacctgg
      121 ctactgtagc gccacggacg ccacccaggc gtttcgagac ggaaagccag acgactggaa
      181 ccctcatgag aatcttgttc atcggaggca ccgggatcat cagctctgcc tgcagtgatc
      241 tcgccctagc acgcggactg gacctttacc tgctcaaccg aggtaagagc gaacttcgcc
      301 ccgcacctcg cggcgcgaag ctcctgagcg ccgacatttc cgaccggcac gcggtcgaaa
      361 atgcgctcgg gtccctgacg ttcgatgccg tcgttaattt cgtggtgttc acggaagacc
      421 aaatccagcg cgacatcgag ctattcggca agcgcaccgc gcaatacgtc ttcatcagct
      481 ccgcatccgc gtatcaaacg ccgcccacgc gcttaccggt cacggagtcc acgccgctgc
      541 acaatccctt ctggcaatac tctcgcaata agattgcgtg tgaagagcgc ttgctccggg
      601 cgcaccgcga gcaaggtttc cccgcgacgg tggttcgccc ctcgcacact tacgatcgga
      661 ctttgtggcc catgcatggc ggctacaccg ctgtggaccg catgcgtcgc ggccgaccgg
      721 tcgtggtgca cggagatggg acgtcgctgt ggacattgac gcatcaccgg gacttcgcgg
      781 cggcgttcgt accgctgctc ggccatgacg gtgcgatcgg cgaagcgttt catatcacct
      841 cggatgagtg gctcacctgg aaccagatcc acgagatcgt ggcgcgggcc gcgggcactc
      901 gcgctgaact cgtgcacgtg ccttcggagg tgatcgcaac ccatgatccc gaatggggag
      961 cgagcctgct gggggacaag acgcacagca tggtgttcga caacaccaag atcaaacgac
     1021 tggtccccga ctatcgcgct agcatcccct tctcacaggg cgtgcacgag atcgtggggt
     1081 tttacgacca gaacccacag catcgtcaga ttgatcgaga gctcgatcgc ttgatggaca
     1141 agttgatcga ctcccaggtt cgagagcggc gggcgctcta gccatagctc tggcggggat
     1201 aggaaggccg tcgacacccg tcgtcgacgg ccgctctcat cgaaaagcgc gaggcctcag
     1261 ggcgacgaaa ctagcccaat cttcgccgta tacgtcagct taccgaggcc ttcgtcctcg
     1321 atctcgaggc tgtagtcggt gggcacccca atccatcctt ccttgcgacc tcgaaacagt
     1381 ggtcgaactc cgcgctcacg accggtaaac gtcaccttca cgcgtgaatc cggtgcttcc
     1441 gtgagcacaa caccaccaac gtacagctct cgcagcgtgg aaccgctgcg tttgtagatc
     1501 gccagagagt aatcggagcc ggtcttcttg aagcgcagca cgtctggcaa gaactgatcg
     1561 cgagctttct ccagacaggc ggaaatcgcg gcaccgtccg gctccttgtc tttgcaggct
     1621 cccagcgctg cctcgcgagc tccggaattg gcgtagtcca cgacgtaagc taggtcgggc
     1681 gcactgagca cctccacgcg cggccgcttc ggaactgggg ttggagtcgg ctcggggctg
     1741 accgtttccg gcggttcagc catcggaggt gggggctgca cgccggctga atcggtgccg
     1801 gtaacggtcg aatcgacgct cgcagcaggc ggattctgat tcgacgaggc cggcggtgcg
     1861 ggcagcccag agctcgtggt tggcgctgga ggagtggctg cagacgcggt ggattgcgtt
     1921 gccgacggag ccgagctcgc gcagcccgcc gctaacgtaa gggccacgca tgcgaagact
     1981 ccaaacatga gctggccata tttctcacac ctcggagtag atgacgggac agtcgaactc
     2041 agcatctcgt actcgccggt tagctcgccg gactctggca ggcccgaacg gcccattacc
     2101 atttacgcgc atactacgct gagcaccacc cccacgaacg gccctgaagc ggggccgcca
     2161 agtgtccttc gggtctcgaa aactggattg aacggttcgc cgagctacgg gtaccaagcg
     2221 ccaggaaccc ggaagcaatg aaacgctcgt cttcgctcca gcagctgatc gtggcttggg
     2281 ccgcgccata cctcgtggtc gcagctccga ctttgacctt gtgccctcgc ccggcgctag
     2341 cccaaggtga aagccgcgcc gagccaacca cgagcgagcc gctcaagatc cacgtggcgc
     2401 tctcctcccc cgcgctcgac gctgagcgtg tgcgtcgtga agtggcgggg gagctgcggc
     2461 gcgaagtggt gctcgcctcg ggcgacgagg cgacgctcac gatcaccgag tccgctgcca
     2521 acgccgccag cccgtcgcgt gctcaacgtg tcggcgcgac gctgcaattc cggtacgtga
     2581 cgagggaagg ccgagtgctc acgcgtgcca tggatttgcc gcgcgatccg aagaaggcgc
     2641 tgcaaatgat cgccctcttg tcgggcaacc tcgcccggga tcaaacctcc gacttgatcg
     2701 gtgcgctgca cgatcaacga acggctgccc tgagtcctag cgcggccgtg gaggcggagc
     2761 ctgcacgcac tgacgcgaca cccgcccctc ccgagcccgc cgccaacgac tcgcaagccc
     2821 ctcccccttc cgcacacgaa cagggtggga acaagcagca cgagggtgca cggagagacg
     2881 aggggtccac cgcgaagtcg ggcgccgcag cccacgagga gtccaagccg gcacagtcca
     2941 agcccaccgc gcctcgatcc tatgcccccc tgaacctctc gctgtggcac ccactgagcc
     3001 tcaatccgaa cagccacgag caaggctatc ggttcgagct cggactcgcc tacagccgca
     3061 tcggcgcgct cgggggagcc gccttctcgg cgggtgttct gcgggtagat ggcccggtcg
     3121 cgggggccgc cttctcgtcg ctctggctca gctccgcggg cccgctgcgc ggcttgattg
     3181 cgagcgggat atttgtgcaa aaccacgggg attcggaggg cgcgcagttc agcggtgtgg
     3241 tcgcccatca tgccgaaggc gaattcaccg gagcccaatt cgcgggcgcc gtcagcctcg
     3301 ccggagacct cgtgggcatc caagccagtg gtgcgtattc acagagtcaa cgcctgcggg
     3361 gcgcacagtt cgcgggagcc gcaactcaca cgaaggaggc ttcccacggc atcgtcggcg
     3421 cgggagccgt gacctggacg cactccttga aaggagcggc tttcgccggc gccctgtccg
     3481 tcagcgaaga cgtggacggc cttgtggccg caggcgccgc cacggtagct cgcgacgttc
     3541 agggcctcgc ggtcgcgggt ggcgtcaacg ttcaacgcga tgtttcgggc gtggcgttgg
     3601 caaccgtcaa tgttcagcgc cgcgttcgag gcttgcagat cggcgtggtg aacgtggccg
     3661 agcgcgtcga tggggcggcg attggtctca tcaacctagc ggggaacggg cgcgtgtctg
     3721 ccctcggttg ggtcgaccaa gacgccgcat acggcggcgt gcgtttcatc agcggcatga
     3781 gttacaccga gctcgcgttc gggcacgagt tcgactccaa gcgttacgtc accgacgccg
     3841 gcttgggggt tcacgtaccg ctcgggcaac gcatcaccct cagcgtcggt ggagccttct
     3901 ccgaaacccg cacccgttgg gacgaagacc aagatcacgc cgaggccatc ggcagaggca
     3961 gtgtgggcct acgcctgacc ccgatggtcg agctcttcgc gggggccggc ctacgctacg
     4021 gcgtactggg ggacaccaaa gaagagttcc gtccggaggg caggctcggg gtggccatct
     4081 tctaaccgtt cgtttttcta gggattcatc caaaagctcg ggcctcgcaa atttcattga
     4141 acggaccgct cgcaaccggg caccaacctc cgctcgttcg acaccctcat tcgatgcgag
     4201 ccccgagcgg tgccgaaccc aaggacttgg accggtgctc ccactcggtt ttcacccctc
     4261 gctcgtctcc tcccccttct acggcacccg cttgacccct ctagcttttg cctcgaatgc
     4321 gacgctagga agcagtcgcg tggacgccga gttggccctc atcgagcgca ttgctgacgc
     4381 ggagccgcgc gccgtcggag aagcatacga cgcacaccac ggtgccgtgc gcgccttcgc
     4441 ggagcgtctg gtgggcgaag ccgcggcggc cgaagatctg gtccacgagg tgttcttggc
     4501 gttgccgagc gcggcccaga agttcgaagg ccgctcgtcg ctgcgcacgt tcctgatttc
     4561 tatcgcggtg aaccgcgcgc accacttcgt gcgcaaagcc agccgtcgac gcaaagccct
     4621 cgatcggctc gaaaaagagc cttcccccgg caatagcacg cccgaacact tgaccgaacg
     4681 ccggcagctc gcccacgctt tgagtcgcgc gctcgacact ctgcccgtcg atcagcgcgt
     4741 ggccttcgtg ctgtgtgaag tcgaggatcg ttcgtcgcgg gaagccgccg aaatcatgag
     4801 cgtaccggaa ggcacggtgc gcacgcgcgt cttccacgcc aaacgcaagc tccgcatcga
     4861 gctcgagcgg gaggggttcc gatgaaccac gacctgctga aagcggcgac gcgcgcgttg
     4921 cgcgaggaga cggagcgtcc cgatcaggat ggcagcgaga ctcgggcgcg cctgatggcc
     4981 agcctgcatc agacgcgcgt ccggaagcgt acccggttgg cgttcgcatt gcccttggcg
     5041 gcgtcgttcg tagccgccac ggcgctcggt cggctcgatc agcgcattcc gcagttggta
     5101 cgttccgtcg ctcactccct gggttggtca gcgaataccg gagataccgc cccgcctcct
     5161 tcgcctcgta agaacgttgg ctccaccggc agtaaactca gtccgacccg caccacgccc
     5221 actcggaatc tagctcccgt cgagagcgtt gtgcctccga ccgagccggt cgcaacaccg
     5281 ctcgatgctc cgacttcgcc gcaagctccc gagattccgc cgagtgcatc gagcggagcg
     5341 gcgccgcgtg cgggcggcaa tccgccgccg caattgcccg cgccgcgagc ttccgcaacc
     5401 gagcaagacg ccggcctgac ccgagaactc gacttgtacc gcaccgccca tcgggctcac
     5461 ttcgaacagc acgattacgt acgcgcgctt cagggctggg accggtactt gaggctcatg
     5521 ccaaacggcc gcttcgcccc cgaggctcgc tacaaccgcg gcgtgtgttt gttgcgcctg
     5581 gggcgcgagg ccgaggccca gcaagcgctc gccccctttg cttctggcca gtacggagag
     5641 taccgccgca gcgaagccca ggcagtcatc gaagcgctgc aacgctgaag ctcgcgcctc
     5701 ggacgccttt gccgtcgggg ctcaacgagg ccaaactcgg gcgtgcgctc gcgagagaac
     5761 ctttcggccc tggtcctgac ctgcggcctc tggtttgggt gcgcgcgctc cgacgcctgg
     5821 gatacccagg tcgagcacga cgtggtcttg ccggagttcc cgaccgagag ctgcggaccc
     5881 caggtgctgc aggtcaccac cgaacacccc aaggtgttac tactgctcga tcgctccgca
     5941 tccatgcagg atccgccgga gaccgaagat ccggacgtcg tgggcaccac ctccaagtgg
     6001 gacctcgtgc tcccggcctt ggacgaagtg atcgcccaaa ccgataccgc gttggattgg
     6061 ggcatgaaga gcttcccgga gggtgacggc accgcggagt gcgccgcaga atccgtcacc
     6121 agccgcatcg acgtgagcgt agcctcgagc aacgctagcc gagtcgtcga gcagattggg
     6181 ctcacgacac cgcaaggttc gggcaccccg accggtgacg ccgtgcggag tgcactcgcc
     6241 tatttgctca cgttgcctgc gggcccgccc aagttcctga ttctagcgac cgatggcgag
     6301 ccctcctgcg ccggcaccac caagggccag gagaccgcgc gcccctacgc catcgaagcg
     6361 ctgcagcagg ctctctcgag cggcgtgccg accttcgtcg tcggggtcgc gaccaacaaa
     6421 gagagcgcca ccaatgcgct gaacgcgatg gcagtggcgg gtggacagcc gcgcgcggtg
     6481 acgcgcgaca cggatcctcg ttattacctc ggcaacacgc aggacgagct gctctccgcg
     6541 ctttcggaga tcaccgccga cctcaccggc tcctgcagca tcgtcttgca tcggccaccc
     6601 gaaagcccag agctcgcgcg cctgcgcata tccggggccg aggtgccccg cgacctcacc
     6661 caccaagagg gctgggattt cgtgggcacc gagcggattc aaacgttcgg cgccgcctgc
     6721 gaccgtacgc gaaaagccgc gcccggcagc ctcgaaatcg tgtacggctg tgtgcaggcc
     6781 ctgccctagc gggagggtcg accgcaaccg agcatgaaag aacgtctgca gggtttcttg
     6841 ttggtgatgg ccgtcggcgc ggccgtcgcg attggggcca agtacctctc cgtcccctac
     6901 aacgtcgcgc tggtcgtggt gggcttgttc ttggtgttcg tggacgtgct gccgcacgcc
     6961 tccatggatc cgcaagtcat cttggtgctc ttcttgccgc tgctggtatt cgaagccgcg
     7021 ctgttctccg acgccaagag cctacgccgt gcgagccggc cgatcctggc cctcgcgatt
     7081 ccgggcgtgg ttatctcgct gatcggcacg gcggccgtcg ccgcgttcgc gttggcgctg
     7141 ccgttctccg ccgcgctgct gctcggagcc gtgctctcga tcacggacac catcagcgtg
     7201 gtcttggctt tccgcagcgt gcgcgtgccg catcgtctgc acgccatcat ggagggtgag
     7261 agcctattca acgacggcac cgcgctggtg ctcgtcagcg tcacgtccgc gatcctactg
     7321 cacggcgtgt tcgatccggt gagcacgctg cgcacgctgc tggtcgcgat cctgggcggc
     7381 gtcgcggtag gttcgatgat cggggcgctc ggcgcagccg tgctgcggcg cacccccgac
     7441 catctcaccg cgatcctcgc gtccatcgtg ctcgtgctcg gcacctcggc gctcgcagaa
     7501 gaggtgcatg cctcacccgt gatcgcggtc gtggtcgccg ggctattcgt cgggaacgcc
     7561 gcacgctctg cgctcgaacc ctcccgtgtg ctcgcgctgc agggcttctg ggaggtcacc
     7621 ggcttcgtga tcaacgtgct cttgttcttg cgcgtcggca tgcagctcga cccgaagatc
     7681 ttgatcacgg aagcgccgtc gattgccctc gcgctagtcg cgcttcacct cggacgggcc
     7741 gtcgccgttt atggttgctt cctcggcctg cagtggttcg caaacgagaa cgtgccgcgc
     7801 aattggcagc acatcatggt ggtcggcaac atcaagggcg cgctctccat ggccgccgtg
     7861 ctcgcgctgc cgcacaacgt tccataccga caccgcctga tcgccatcgt gttcggcgcc
     7921 acgctggtca ccatggtcac gcaagcgctg ccgttccgcc gcttcctgca ctggttcaag
     7981 gtcgtggaag ggggcaacga ggcccgcatg gacgccgcgc gggcttccct gattgcggcg
     8041 cgccggggcc agctcgagct cgacgatttg ctcgagtccg gcttgttgtc tcgccgctcc
     8101 cacgcagagc ggcgcgcgca gttccagcgg cgaatcatcg cggcggaggc gatcctccac
     8161 gaagtcgaag gcgagcgccc ccgtgaccgc ctggtggaca ccgcgctgct ctatgctcag
     8221 aaggcctccg tgctggatgc ggcacgccgc gggctcgtac ccgagccgat cgccaacgcc
     8281 gaggtcgagc gcatcgacag cagcatcctc gacctcgagc tgaaccacga ccacgacgaa
     8341 gaacacggac ccgcaagctc ggccgcccct gcccacggcg ccaagccgca gccgcgcgag
     8401 ggcctcccgc ccgaacgtcg accggcgttc tattccttga gccccgatcg tcggcacgaa
     8461 aaggatcctc catgatgcga gtcttcatcg cgggcgcggg ccaagccggc gtcagcgtcg
     8521 ccaagcacct gagcaacctg ggccatgaag tcagcgtggt cgatcgcgac ccgctgatta
     8581 cccgtcgcat cttcgaacaa cacggcatcg ccgcgatcac cggggacgct acggacgcgg
     8641 cgctgctcca acaagcggag attggacgcg ccgacgtggc cgtctccatg ctggcccgcg
     8701 acgcagacaa ccttgccgtc gccttgctag cgcgtgaatt cggcgcccgt cgcgtgatgg
     8761 tccgcatgcg cgacgtcgcc tatcgccggc cctacgagca cgccggcgta gaccggatcc
     8821 tgtccgagac cgagattctg gtcggctcgc tcgccatctc catcgagcac gaagccgtcg
     8881 gccacgccat gatcctcggg gacggcaact cggtcgcgtt cgacttgacc atcaaccatg
     8941 ccgcgcgcgt ggtgggccgc accgtgagcg agctcgcgca gtccgcggat tttccccgct
     9001 cctgcgtgtt cgccgccatc ttcgacgaaa acggcatgac cagcccgcgc ggcaataccc
     9061 aaatccgcgg gggccaacag gtgttgctgg tttcgcaccg ggaagagatg agccgggtcg
     9121 tggacttctt catgagccgc gcctgagctg gcgttattgg gtggggcttg cgcggcatcg
     9181 cgtgctcctg ggagggccgc ggtggtgcgg gtgcggaatc caacgctcag tcgcgccgcg
     9241 tcatttcctg ccccgcaaat tcatacagcc cgcaaattga cataagcact cgcttgggtc
     9301 gggccgcgca actacgagcg cgctgctcga aggttcgcgg cgcacgcgca gcctctgacg
     9361 cgcgcgacat cccccaacac cacgccggcc cccctcggca acgcgcagac gacggcattt
     9421 tcgttcgaaa actacgcagt tccggccgcg atcattttcg gcgtgcatag cgcgaaaacg
     9481 acctaacgtc ggggctcgtg tcggatgtca ctaaagtgac cacttccgcg cctgctcgtg
     9541 aacgcgacca acacaaacta agagcccacg aggagcgccg gagacggcgg ccgatctctc
     9601 ggctgccggc aactagctcc tctggggaca atccaaacgc tgcgtggaaa tcctgcgcag
     9661 cgtcgcagtc gcgcccgagg gccgaggcac gcattcgtca cgtgagagcg gtcgtgtgcg
     9721 cgctcgcccc gtcgggcaaa cgcatctcga tcgccacctt tcgtgagcag agccagcaag
     9781 cgctttcgtc aggcgcacga gtcgggactt ttcgaggagt agccgatgaa catgcgaagt
     9841 cttagttcga gtctatttgt gggagcggtt ctagttttcg cggcttcgtg cgggtcggac
     9901 aagaacgatc ccccgcccag ctccggaacg ggcggggcga gcgcaggcgc gaccagcagc
     9961 ggaggcgcga gctcgggcgg catggcgagc accacgggcg caaccgcagg cggcggcgcg
    10021 accagcacgg ccggcgctgc cacagcgggc gggcgaacga acgtcggtgg cagtagcgca
    10081 accggtggca ccgcgggtgc tggcggcaaa ggcgggcagg gcgcgtttgg ttgccccgcc
    10141 gaaaagccgg ccgcgggcgg cgaatgcacg cggatggcca acgtggcctg tacttatgac
    10201 gacggcggct gcttgtgcgt cgacggcgcg tggtcttgtt actcggacgc agactgtccc
    10261 acagccgcac ccgcggacgc ggcgacgtgc acactcggcg ggatgcagtg tgctttcgat
    10321 aacgtgaact gtacctgctc gacgaccaac ggctggacgt gtgagagcgc ctgcccgagc
    10381 gctccgcccg ctgctgacgc agcgtgccgt cgcgccgtca acagaacctg tcgttacgcc
    10441 gagggcgcgc tagtgcaggg attcggcacg ccggacacga cgtgcgcgtg caacgagagc
    10501 aaattcagct gcttctcgca agacgactgc ccggcggccg caccggagaa caccagcgcg
    10561 tgcacgatga acacgctgag ctgcgagtac gacgaccgtc agtgcacctg cacgaacggt
    10621 gaatggaatt gtgtcaccga ctgccccgag gcagagccga gtgacggcgc cgtctgcatg
    10681 cgaccggaga acgccacctg ccgttacgcg gagaaggcat tgttgcaggg cttcggaggc
    10741 aaagcggact ccacctgtgc ttgcgcggac ggaaaattca gctgttttgg gcaagaggat
    10801 tgtccggcgg cggcgccgag cagcggcggc acctgcgagt tcgcgagctt ggcttgttca
    10861 tacacgggtg cgcagtgctc gtgttccaac gacaacacgt ggcgctgcac caccgattgc
    10921 ccggcggctc cccccgctca ggacgcggct tgtgatcgac cggagacatc ggtttgccgc
    10981 tataacgacg gggcgctggc ccagggcggg ttcggtgcgg ccgaggcgct gtgtgcttgc
    11041 cgcgagaata agttcgactg cctgacgaag gccgactgcc ccgccactgc gcccgccaat
    11101 caagcggagt gccctgacct taccaacctg agctgcagct atacggatca aacctgcact
    11161 tgcggcatga acggctggac ctgccagtcg gagtgcccgg cgaaggcccc cgcggccggc
    11221 gcggcttgct ctcgcccagt cacgtccgcc tgcctgtacg tcgcgggctc gttggtggag
    11281 tcgggcaccg atccggagag cacctgcgtg tgcgcctcca atgcgttcgt ttgctacacg
    11341 gacgcggatt gcccggcggc gcagccggag agcaacgcgg cctgcgagaa gcccggcttg
    11401 gagtgcgcgt tcggagatca aaactgccgt tgccgcacct cgacgaacgc ttggtcttgt
    11461 aacgccgccc ccccgcccat gggcgccggt ggagccgcga actaagcctt agtcaccggc
    11521 attcccaggc agaaaactcg ggtaaccagc gcgccggagg ggggcgctgg ttacccggcg
    11581 cttggcgcgc cggcctcgcg gctttgccga gcgggacgag tgagattaaa gaggccgagc
    11641 cgtgcctcgt accgactctc gccgccgcga actcctgacc cgacttgctg cccagtacgg
    11701 aacaccgctc tacgtctacg acgcgcaggt gatccgtgcc cagttcgcga agctgcgtcg
    11761 cttcgacgtg gtgcggtttg cgcagaaggc gtgtcccaat acgcacgtgc tgcgcctgct
    11821 gaagggcgaa ggggcgatgg ttgactgtgt ttccgcgggt gagctcgagc gggccgtgca
    11881 cgcgggattt agtgggggtt ccgagcccgc agaaatcgtg ttcaccgcgg atgtgctgcc
    11941 gcgcgaggtg agtgagcgcg tggtggagct cggtgtaccc gtcaacgcag gctccgaaga
    12001 catgttggag cagctcggcc gcgtgagccc tgggcacaag gtttggcttc ggatcaaccc
    12061 gggctttggt cacggccaca gcaaaaagac cagcacgggc ggagaaaaca gcaagcacgg
    12121 gatctggtac gccaacctgc agaaggccct ggttcacatc gatcgctacg gcttggatct
    12181 agtgggcttg cacacgcaca tcggctcggg cgcggacatc gagcacctgg gtcgggtgtg
    12241 cgacgcgatg gtggaacagg tgcggaacct cgggcgcgat atccgcgcca tctcgggcgg
    12301 cggggggctc tcgattccct atcgcccgaa cgatccccag gtggacacgg acgcctattt
    12361 caagcgttgg gacgctgccc gcaagacaat cgaaggattt ttggggcatc cggtgcacct
    12421 cgagctcgag ccgggtcggt tcctgaccgc ggccgcgggc gtgctcgtcg cgcaggtgat
    12481 ggccagcaag accgcgggca gcaatcacta cacgctcgtg gacgcgggct tcaacgatct
    12541 ggtccgcccg gccatgtacg gcgcgtggca cgagatcgag gtgctccccg catccgatcg
    12601 aaaagacggt gagttgctgc ccacgattgt gggcggcccg ttgtgtgagt cgggagacat
    12661 gttcacgcag tcgaaggacg gtgacgtgct gccgcggaac ttaccccgta gcgaagtcgg
    12721 cgacctgctc gtgttccacg acgccggtgc ctacggagct agcatgtcga gcaactacaa
    12781 ctctcgcccg ctcgcggccg aggtgctgat cgacggcgac gatcatcgct tgatccgtcg
    12841 gcgtcaaagc atccaagacc tactcgcgtt ggaagcaacc tgacggcagc cacccgggcg
    12901 gcgcggtcag gggcggcgcg gtcaggtggg agggtctcta tctgcgcccg atttcgagcc
    12961 gctcggagtt gtcgggctac tgtgcgaact cggagagggc gagctcgacg tgcccgagct
    13021 ccgaacgccc gaatgcgctt gggatgattg ggcgtctcca ccgggcgagg gaaagggccg
    13081 gcgcgagggc gcgtgcaggc gcttttcgag cagcgcgatg cgcgccagca tctcggtctg
    13141 gcctttgatc aaggcttgga tcccgagctc gctcttgaga tttacctcgt agtcgttggc
    13201 ggccagcagc ttgtccttcg ccgcctgtcg gttctggctc atcatgatta ccggcgcctg
    13261 gatcgcggcc agcgtcgata gaatcaggtt gagcaggatg aacgggtaca cgtcgaaggc
    13321 gtgcccggag aaggcgttga acgacatcca cacggccatt cccaacgcga acacaccgat
    13381 gaacggccaa gatccaccga atgcggcgac gcgatcggcc acgcgttccc cgagcgtcag
    13441 cttctcttcc tgctcttcga gcacgttgcg cgtgacctga ccggaaaagg cggcgttcat
    13501 ctggcgcaag cgcgcggcga gctccccggc gatttgcagc gtcgcctccg gatggttggt
    13561 ggtgaactcg acgaagtctt cgcgatcgag cgccaacagc ttgcaggcgc ggctggcggt
    13621 ggccgcggcc gagcgcggct cgccgtcgaa cagcgagagt tccccgaagt attggccggg
    13681 agcgagagta gctaaccgca cgcggtgggg cccctccccg tgggagatgt cgaccaagcc
    13741 ttccttgatg atgtagaggc gcccgccctt gtccccctgc gcgaagatca acgcgccttc
    13801 cgggatctcc accagctcga gtcgagacga caactgctcg agctcgctct ccgcgagcga
    13861 ctcgaacagg ttcagggaac cgagcaaggt gctgtactcg agagcgatac gcatactccc
    13921 gtcgctgctc gcgccgggtt cgttgctcgc gcgcggtggc tgaagactag ctggcggctt
    13981 tcgggatggg cgggcctgct gcatgaccac tcccagaggc tgagctcatg aagggtttct
    14041 cgagaacgag gaagaacaga taagcacagc cgagcaccgc gaggagcccg aaactaagca
    14101 gcacggaaaa catggcttcc ggagccaacc ccagcggcga gagcacgtat tgccacatta
    14161 gctgcaacag cggcgcgtgc agcaaataga cgctgtagga gaacgtgccc acgaaggcca
    14221 acggcttgaa tgcaaacaca ctgcgcagcc ggctttgcgg caggcgtgaa gtcaaaacca
    14281 gcaggcaggt ggcgaagatc ccgaccggca tgtccagcca gtaaaagctg tggatagccg
    14341 agcggaaacc ccagtacatc gcgagggcca cggccgtcac gaacgcgaca ctccccaccg
    14401 ccgcccacgg gaaaccataa cgccagcgct tgaacggctc tcggtcggct tgcgccacgt
    14461 acgccgccaa cattccgagc gagaacatgc cgacgaagtg cgggttggcg cgcgcgatcc
    14521 gcgtatgcgc gaagccgatg cgcagcgcat agccaatcaa caacgccaag cccacgacct
    14581 gcagcgagcc gagccgtcga aagccgagca ccagcagcgg gaacaagaag taaatctgcc
    14641 attcgacggc gatcgaccag aacacgtagt tgatcgagcc ggtggcataa aagtcctgca
    14701 gcaacagggc gtggctcacg atcgagaccc aagtgagctc cagaggaacg tcccaaatcg
    14761 tgccggtctt cttgccgatc agcgtcgcga tcaagagcag cgtaaggacc agtgcgccgt
//
LOCUS       DASTKU010000586         7345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1453731, whole genome shotgun sequence.
ACCESSION   DASTKU010000586 DASTKU010000000
VERSION     DASTKU010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453731"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1135
                     /locus_tag="VFQ61_20540"
     CDS             230..1135
                     /locus_tag="VFQ61_20540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956902.1"
                     /translation="MTDAFIRYLSAKRTVDDRALNRVVLERFRSDLQKLRAPRILEIG
                     AGTGTMVDRLWNWNVIASGEYTLLDQDESLLEEAKRRLMRLAAEKGGRSTLNPDGSLS
                     VSAANVDLTIRTYAAELGSFLRQHSDTRYDVLIACAVLDLVDVPEALPKLWSKLRAKG
                     LFWFSINFDGESIFLPEHPLDERVVGVYHASMNDRIRDGKPSGDSKTGRHLFTHLKAC
                     GAHVLVSGSSDWVVHSVGGEYEGDEQAFLRHIVEFFRAELSQHAEIDEKELENWVAMR
                     NRQIDEGDLVYIAHQLDFVGSAPSL"
     gene            1201..2115
                     /locus_tag="VFQ61_20545"
     CDS             1201..2115
                     /locus_tag="VFQ61_20545"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9956903.1"
                     /translation="MLSEPGLDNPQVRSVANYYDENTRAFVRRGHGRTTGAIHRAVWG
                     PGVHTREDAMHHVEDRLLRYWLDRSAGREREQPAYFLDLGCGIGGSLCSLASKAARLG
                     LVKFSGVGVTISSVQVEIAASFIAQRGWSDRVQCIQGDYCELPDRIPPDADVAFAIES
                     FVHGPSPTRFFSEAARMLKPGGLLMVCDDLLASPLESLIEPTSRLAERYRVGWQAVTL
                     VTEQQLLAEAGKAGLTHAATWDLTGHLELDRPRDRAISVLVALLGELPIRSSYFGMLK
                     GGDALQKGLKAGWLQYKAVLLEKPRRAE"
     gene            2159..2890
                     /locus_tag="VFQ61_20550"
     CDS             2159..2890
                     /locus_tag="VFQ61_20550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956904.1"
                     /translation="MRKAALRFLVSSIGFTVACNALLGNEEGILDPRRPDVENSGGSD
                     SNQGGGSSRSSGGSAGSSTTGGESTARGGAEDGGGPAEVGGSGASGAGGADSTSGGEP
                     SMGGTVSMGGTVSMGGAVSMGGAVSMGGMGSGGQPCVCTPGAQSAERVAPCPNGGWKY
                     QKQTCSDKCQWGAWTDTKACQYPQNECTGCACVSDCKDSVTGGTQCLWLACSEQEGRT
                     ECDADIQRVCGTKTQPFEFKDWRVQ"
     gene            complement(2914..3738)
                     /locus_tag="VFQ61_20555"
     CDS             complement(2914..3738)
                     /locus_tag="VFQ61_20555"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9956905.1"
                     /translation="MSRDRCSVPLPARGILGGESPEGYKQGVRVPPPPSLAAYIHHFW
                     FVRWALSRPLAAAALSYPTVVLTFEEHAGVKRAEITGPLTERLTKRLSGEGLAFGITF
                     RPGAFSPLFEGRMAALANRTLALVDVFGAEGGAWADAIFEERDLDGRIERATEFLERR
                     LPPMSAEVRRVRDIVEQMATNRSLLRVEDVCVATGLDLRTLQRRFQQRVGVSPKWALR
                     RFRMLEVVEQLKAPDRPSLAQLAASLRYADQAHFAREFKRVVGETPSRFVQYGGVV"
     gene            3810..4580
                     /locus_tag="VFQ61_20560"
     CDS             3810..4580
                     /locus_tag="VFQ61_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9956906.1"
                     /translation="MKLTGEHCENGVSERRFELEVAGAAVPGVLWTPEGARGPRPLIL
                     FGHGGGQHKKAAPVLGTAMRYAKALGYAIVAIDAPDHGARVSPQDAPQVAQRERELII
                     RAGGMRGEALESAMRRAAIVQAEWAATLDAVLTLDIVGSHGSVGYLGLSMGAILGLPF
                     IASEPRVKAAALGLVGAYGADHPLLQAARRITIPVEFVMQWDDTFVPREDALTLFAAF
                     ASSEKSLHANPGDHADVPAFERTSRENFFVRHFGMVNG"
     gene            4952..5194
                     /locus_tag="VFQ61_20565"
     CDS             4952..5194
                     /locus_tag="VFQ61_20565"
                     /inference="COORDINATES: protein motif:HMM:NF015640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HET9956907.1"
                     /translation="MSHVELPEDLVRLAEAQVAAGRASDLGEVLRLGLAVIERRRQRK
                     EQELFALREALAEGDASPDFQGDPFATVRAEFGLSK"
     gene            5204..5509
                     /locus_tag="VFQ61_20570"
     CDS             5204..5509
                     /locus_tag="VFQ61_20570"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET9956908.1"
                     /translation="MSFRFSSRAVEDLRAIAAYTLKSWGPEQCQRYLQSLEVACQLLA
                     EDPALGRPCEHLSSGLWRRERGRHVIFYRVTEYGIRVVAILHERMMPERHIDEDDDE"
     gene            5764..>7345
                     /locus_tag="VFQ61_20575"
     CDS             5764..>7345
                     /locus_tag="VFQ61_20575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9956909.1"
                     /translation="MRTRLTGAGRRWVWFGLAALWGAGCTSDNKNGGQATCEPGTERC
                     PCDHGACNAGLSCLDQVCVHGTIGTGGVGGKQGGSGGGASGTAAGSSGGTAAASSGAA
                     GARTTGGAEAAGTTGVSGSATAGMSGNAGNGVCGSGGGAAGTTATTGACPPEASDRIV
                     ATTGVDRGDCSIDPCLTFRYAGTQMRAGETLVVEDGTYADSIGSDTFPPGSPSGYTRI
                     VARHPGAVTIEGRLDLYENREFYLELAGLRFVGDESKNVAGGHVRLVGDSFVNGPPDG
                     NAAVLSIGTNDWAPGAWNIEVIDTIVYGHGGRYDVLVYRANDVTLTRLVARKDGGWSG
                     DDQSNPEGVVIFYESSRSVCDRCVVLDSISESGAEPLGGIIVNSHDAKLSTDTEIRAS
                     AVIDSNYPGITLEGHGDVNEARIIDSYASGNSSNGVTLNVGGDLTITRLTAIANRGDG
                     IANYGDITVSSSDCLLRDNSGEDLRGVPGTTTGSGAAPIDFAGFDQARVRRELCAGET
                     RGFCGANLSFAEHVMLRAL"
BASE COUNT         1396 a         2246 c         2360 g         1343 t
ORIGIN      
        1 cggcgagtgc gtgcgacccg gagaggccgc gctggtgacc gttcacgagc cgcaaagacg
       61 gtcggagctc caccgcgagc gcaggctgct cgatcgcggc agccagagac tctcgtcgaa
      121 cgagtttgct cagcccagct agcgaaggag gcagcccagt gcgccgcacc gatggtgtcg
      181 cgaaccctgg tgtcgcgaac gtgctaacgt gcgagcctcg gatcgttgca tgactgacgc
      241 attcattcgc tacttgtccg caaaacgcac tgtagacgat cgcgcactca atcgtgtggt
      301 gctcgagaga tttcgcagcg atttgcaaaa gctccgcgcg ccccgaattc tcgaaattgg
      361 ggccggcacc ggcacgatgg tggatcggct ttggaactgg aacgtgattg ccagtggtga
      421 atacaccttg ctcgatcaag atgagtcttt gctcgaggaa gccaagcgac gcttgatgcg
      481 cctcgcggcg gagaaagggg gcaggtcgac gctgaacccg gatgggtccc tctccgtatc
      541 tgcagccaat gtcgacttga cgattcgcac gtacgcagca gagttaggca gtttccttcg
      601 gcagcacagc gatactcggt atgacgttct gattgcttgt gcggttctgg acttggttga
      661 cgtcccagaa gcattgccca agctttggtc gaaacttcgc gcgaagggcc tattctggtt
      721 ctcgatcaac ttcgacggtg agtcgatctt cctgcccgag catccactcg atgaacgcgt
      781 agttggcgtg taccacgcca gcatgaatga tcggatccgc gacggaaagc cctcgggaga
      841 tagcaagacg gggcgtcact tgttcacgca tctgaaagcg tgcggcgcgc acgtgctcgt
      901 cagcggcagc tcggattggg tcgtgcacag cgtaggcggc gagtacgagg gcgatgagca
      961 agcctttctt cgtcatatag tggagttctt cagagccgag ctctcccaac acgcagagat
     1021 cgacgagaaa gagctcgaga actgggtcgc catgcgtaac cgtcaaatcg atgaaggcga
     1081 tctcgtttac atcgctcacc aattggattt cgtcgggagt gcacccagcc tttgatatag
     1141 atcgctcacc gtcggcagaa tcagctggcc tgcgttgaaa gccccggcta acttcccggc
     1201 atgctgtctg agccgggcct cgacaaccct caagttcgaa gtgttgcgaa ctactacgac
     1261 gagaacactc gggccttcgt gagacgggga catggccgca ccacaggcgc gattcaccgc
     1321 gcagtttggg gccccggggt gcatacgcga gaggacgcca tgcaccacgt cgaagatcgt
     1381 ctactccgct actggctgga ccggagtgcg ggtcgcgaac gcgagcagcc ggcctatttt
     1441 ctcgaccttg gctgtggaat cggagggagt ttatgctcgc tcgcgtcgaa agcggcgcgg
     1501 ctcgggctgg taaagttctc gggagtgggc gtcaccatca gctcggttca ggtcgagatc
     1561 gcggcctcct tcatcgctca acggggctgg tcggatcgcg tgcagtgtat acagggagac
     1621 tattgtgaac ttcccgaccg gatccctccc gatgcggacg tggcgtttgc gatcgagtcc
     1681 ttcgtgcatg gtcctagtcc aacccgattc ttctctgagg ctgcgcggat gctaaaaccc
     1741 ggaggcctct tgatggtttg cgatgacctg ctcgcctccc ctctcgaaag cctaatcgag
     1801 ccaacgtccc ggttagcaga acggtaccga gtaggctggc aggcagtcac gttggtcacc
     1861 gaacagcagc tccttgctga agcaggcaag gcagggctaa cccacgcggc aacctgggac
     1921 ctcaccggtc acctggaact cgaccggcct cgtgatcgcg cgatttcggt gctggtggcg
     1981 ctgctcgggg agctaccaat tcgatcgagc tactttggca tgctgaaagg tggcgatgcc
     2041 ctccagaaag ggctcaaagc tggatggctt cagtacaagg cggtgttgct cgaaaagccc
     2101 cgccgcgccg agtgaccgaa gactgacgtt tccaccctgg cgtgatagcc tccagtccgt
     2161 gcggaaggct gcattgcgct tcttggtctc gagtattggc ttcacggtcg cttgcaatgc
     2221 cctcttggga aacgaggagg gaatcctcga tccacggcga cctgacgtgg aaaattctgg
     2281 cggctccgat agcaaccagg gcggcggttc atcacgaagc agtggcggct cggctggctc
     2341 gtcgacgact ggcggtgagt caacggcgcg aggcggagcg gaagacggag gtggccctgc
     2401 cgaagtgggt ggctccggcg cctcaggagc gggcggagca gactcgacgt ccggcggtga
     2461 gccgagcatg ggcggcactg tttcgatggg cggcactgtt tcgatgggcg gcgctgtttc
     2521 gatgggcggc gctgtttcga tgggtgggat gggctctggc ggccagccct gcgtctgtac
     2581 tcccggcgcg cagtccgccg agcgcgtcgc gccgtgtccg aacggcggct ggaagtacca
     2641 aaagcagact tgcagtgaca agtgccaatg gggcgcgtgg acggacacca aggcttgtca
     2701 atatccacag aatgaatgca ccgggtgcgc ctgcgtgtcc gactgcaagg attcggtgac
     2761 gggcgggacg cagtgcctct ggctcgcttg cagcgaacaa gagggacgta ccgaatgcga
     2821 cgccgacatc cagcgagtct gtggcacgaa aacgcagccc ttcgaattca aagactggcg
     2881 tgtgcagtga ctcgcggccc atcggtatgt gcattagaca actccgccgt attggacgaa
     2941 ccgactcgga gtctcaccga caacgcgctt gaattcccga gcgaagtggg cttgatctgc
     3001 gtaccgcagg gaggccgcga gctgcgccag cgaggggcgg tcaggggctt tgagttgctc
     3061 cacgacctcg agcatacgga aacgccgcag cgcccactta gggctcacgc ccacccgttg
     3121 ctggaaccgt cgctgcagcg tgcgcaggtc gagcccggtt gccacacaga catcctcgac
     3181 ccgtaacaac gaacgattgg tggccatctg ctccacgatg tctcggacgc gccgtacttc
     3241 ggctgacatc ggaggcaggc gtcgctcgag gaactcagtc gcgcgctcga ttcgcccgtc
     3301 gagatcgcgc tcctcgaaaa tggcgtctgc ccaagcaccg ccttctgcgc cgaacacgtc
     3361 aacgagcgcc aacgtgcggt tggccaacgc cgccatccgc ccttcgaaaa gtggactaaa
     3421 tgcccccggc cggaatgtga tcccgaacgc cagtccttca ccagacagcc gcttcgtcag
     3481 acgctccgtg agtggacccg tgatctctgc acgtttgacg cccgcatgct cctcgaacgt
     3541 caacaccacc gttggataag agagtgccgc ggccgcgagc ggcctgctca gcgcccatcg
     3601 gacaaaccag aaatggtgaa tataggcagc gagcgacggc ggaggtggga cgcgaacacc
     3661 ctgtttgtac ccctccggag actcaccccc gagaattccc cgcgcgggca gcgggaccga
     3721 gcacctgtcg cggctcattc gccctgagtg tgtcccgtgt gtcgcgttta ttcaagacaa
     3781 agccttgccc aacaacgagt ctccttggca tgaaactcac cggcgaacat tgcgagaacg
     3841 gcgtcagcga acggcgattc gagttggagg ttgcaggtgc cgcggtgccg ggcgtgctct
     3901 ggacgcccga aggggcgcgc ggtcctcgcc cgctcattct gtttggccac ggcggcggcc
     3961 aacacaagaa agcggccccc gtcttgggaa cggccatgcg ctacgccaaa gcgctgggtt
     4021 acgccatcgt cgcgatcgac gcgcccgacc acggcgcgcg agtctcgcct caagatgccc
     4081 cccaagttgc gcagcgggaa cgcgagctca ttattcgcgc gggcggcatg cgcggagaag
     4141 cccttgagtc tgccatgcga cgcgcggcca tcgtccaggc ggaatgggcg gcgaccctcg
     4201 acgccgtgct gaccctcgac atcgtcggat cccacggatc cgtaggctac ttgggcctat
     4261 cgatgggtgc gatcttgggg ctcccattca tcgccagcga gccaagggtc aaggccgccg
     4321 cgctcggtct cgtgggcgcg tacggcgccg accatcccct cctccaagcc gcgcgccgaa
     4381 tcaccatccc tgtcgagttc gtcatgcagt gggacgacac gttcgtcccc cgtgaggacg
     4441 cgctcaccct gtttgcggcg ttcgcctcga gtgagaaaag cctccacgcc aaccctggag
     4501 atcacgctga cgtccccgcc ttcgagcgca ccagtcgaga gaacttcttc gttcgccact
     4561 tcgggatggt caacggctga cttgtcctcc tcactgcggc atgggtgagc aagctcaagc
     4621 ctgagttaga ccctgccttg ccctctgcgg cgttccactg gctctgaaga cccaaagcga
     4681 ggagccaacg ctcgatgcat tgatagtcta aggattccag ctttcttctc agaatgcccg
     4741 cgacgtccac aatctggcgc tcgggccgca ccacgcccgc gaagctcgag accgaagcaa
     4801 cgcacctcgc cagctgcaaa cccgagttgg tcacctcgta cacgccgagc ctggccccag
     4861 gtaccgcgcc gggaaagcca gatctcgcta gaggtttgcc aatgtcgcca agttctccca
     4921 tacttctgcg ctatcccccc ccccacagct catgagtcac gttgaacttc ccgaggacct
     4981 ggttcgcctt gccgaagcac aagtcgcggc tggacgcgcg agcgaccttg gcgaagtgct
     5041 gcgcttaggg ctcgcggtga ttgagcgccg tcggcagcgc aaggagcagg agctgtttgc
     5101 gttgcgtgaa gcactggctg aaggagacgc gagccccgac ttccaagggg accctttcgc
     5161 gacggtgcgc gctgagttcg ggctcagcaa gtagggggtg agcttgtcat ttcgtttctc
     5221 ctcgcgggcc gtggaggact tgcgagcaat tgcggcctat accctgaaaa gctggggacc
     5281 cgaacagtgt cagagatact tgcagtcgtt ggaagtagcg tgccaactgc tcgcagaaga
     5341 tccagctctt ggtcgtccgt gcgagcactt gagttccggt ctctggagac gcgagcgtgg
     5401 acgccacgtg atcttctacc gcgtgaccga gtacgggata cgcgtagtcg cgattctcca
     5461 cgagagaatg atgcctgagc ggcatatcga cgaggatgac gacgagtgag ggcgtttgga
     5521 tcgtccatca agctaacacg ccgaccagag gcgccgtact ctccaccggc agatcaagac
     5581 tcgccaagcc tgttcagcag aacctcccag tcctcgtctt cgatctctga cggaacgaaa
     5641 accttcgttg gcagcggtgc aacgtatgcg ttgtaccgcc tgcactcggt tttttgagcg
     5701 caccgttgct cgcaattcgc cgtgacttga acggctgggc cgcgcatgag attgtggtgc
     5761 atcatgcgaa ctcgcttgac cggagctggc cgtcgctggg tttggttcgg acttgcggcg
     5821 ctctggggag ccggttgcac gagcgataac aagaacggcg ggcaggcgac gtgcgagccc
     5881 ggaacggagc gctgcccgtg cgaccacggc gcgtgcaacg cggggctcag ctgcctggat
     5941 caggtgtgtg tgcacgggac gatcggcacc ggcggagtcg gcgggaagca aggcggaagt
     6001 ggcggcgggg cctcgggcac ggccgcgggc agcagtggcg gcacggccgc ggcgagcagt
     6061 ggtgctgcgg gcgcgcgcac gacgggcggt gctgaagctg ccgggacgac cggcgtgagt
     6121 ggcagtgcca cggcaggaat gagcgggaac gccggcaatg gtgtctgcgg ctcggggggc
     6181 ggtgctgctg gcactactgc aactacaggc gcatgccctc ccgaggcttc tgatcgcatc
     6241 gtagcaacca ccggggtcga tcgtggagat tgcagcatcg atccgtgcct cacctttcgc
     6301 tatgcaggca ctcaaatgcg tgcgggcgag acgctggtgg tggaggacgg gacttacgcc
     6361 gactcaatcg gtagcgatac gttcccgccg ggcagcccat cgggctacac gcgcatcgtg
     6421 gcacggcacc cgggtgcggt gacgatcgag ggcaggctcg acctctacga gaacagagag
     6481 ttctatctcg aactcgcggg tcttcgcttc gtcggtgacg aatcgaagaa cgtcgccggc
     6541 ggtcacgtgc ggctcgtcgg cgactctttc gtgaacggtc ccccggatgg aaacgccgcg
     6601 gtgctctcga taggcacgaa cgactgggca cctggcgcgt ggaacatcga agtgatcgat
     6661 accatcgtgt atggccatgg cggccgctat gacgtgctcg tctatcgcgc aaacgacgtg
     6721 accctgacgc gcctcgtggc ccgcaaggac ggcggctgga gcggcgacga tcagagcaat
     6781 cccgaaggcg tagtgatctt ttacgaatct agccgctcgg tatgcgatcg ctgcgtggta
     6841 ctcgacagta tctccgagag cggcgccgag cctctgggcg gcatcatcgt caactctcac
     6901 gacgcgaagc tcagcaccga caccgagatc cgcgcgtcgg ccgtaatcga cagcaactac
     6961 ccgggcatca cccttgaagg tcatggcgac gtgaacgaag cacgcatcat cgatagctac
     7021 gcgagcggca acagcagcaa cggagtgacc ctcaacgtgg gcggggacct gaccatcacg
     7081 agattgaccg cgatcgccaa tcgaggtgac ggcatcgcga actacggtga tatcaccgtg
     7141 agctccagcg attgcctgtt gcgggacaat agcggagagg atctgcgcgg tgtcccgggg
     7201 accaccaccg gcagcggcgc tgcgccgatc gacttcgcgg gcttcgacca ggcccgtgtc
     7261 cgccgcgagc tgtgtgcggg ggagacccgt ggcttctgcg gcgccaacct gagcttcgcg
     7321 gagcacgtta tgttacgcgc gcttc
//
LOCUS       DASTKU010000587         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1230214, whole genome shotgun sequence.
ACCESSION   DASTKU010000587 DASTKU010000000
VERSION     DASTKU010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230214"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..370)
                     /locus_tag="VFQ61_20580"
     CDS             complement(149..370)
                     /locus_tag="VFQ61_20580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956910.1"
                     /translation="MKHETSEDVWLKDRSIDSPWDYDVRLFLEPAFILVEVSVTTASL
                     RQDVAQLLGWLRAQTVVEFTDDDGVPLTL"
     gene            1027..2127
                     /locus_tag="VFQ61_20585"
     CDS             1027..2127
                     /locus_tag="VFQ61_20585"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9956911.1"
                     /translation="MLASPREWARLGSGLAIQAHLALDEFSMAPSHVSTKWGLSWSTV
                     RRAELDAIDRWDRAHPPVPLVMAGVDGKWLGRRHKRKEKFVTIVSNLETGVPVWIGYG
                     RDGATLKSFLGSLTPEQKAALKLFAVDMHEPSKKAIHEDHALAHAAIVHDPFHVIKRA
                     GEAVTELRRAVFFRAGPELREVGRGSRWLVLRAWEKSTDEQQSRLRRLFRLNGKLSEL
                     LTERPTERVALPLCRGMVEVFAVRSKRPAFLDRVHEVTLGKSAGGLRLGTAAGERVEL
                     EHRPERALAELILGTVHRCSLFTARLRGVAPVFGTSLLRDYLNVVGVFWSEQPIEREI
                     FSCQGGGAARPSPRAMRSRQFAPTAGGARSFP"
BASE COUNT          428 a          743 c          696 g          446 t
ORIGIN      
        1 aacgccgtcc ggtcgaagcg tacccggggc gcgatgagcg gcggtgacac gtcgggtcga
       61 gcgtgtacga gctggcgccc ggcgagctgc gagaacgccg gccatggcgg gcacgttggt
      121 caggagcggc tccccatggt ctttggtgtc aaagcgtcaa cggcacgccg tcatcatcgg
      181 tgaactccac gactgtctgg gcgcgcaacc agccgagcaa ctgtgccacg tcttgccgca
      241 ggctcgcggt cgttacacta acttcgacca ggatgaacgc cggctcgaga aatagcctta
      301 catcgtaatc ccacgggctg tcgatggacc ggtccttcag ccacacatct tctgacgttt
      361 catgcttcac gacatgcagt ctctcttcga aaacgtggca agcgccgaac gctcggacac
      421 gaaaagcggg atcgcataga tttcccctgg caccacggtc acactctttt tcatgactta
      481 cgcccggcag ccctttactt tgaggttctt gattgaatcg aggccgcgct cgttgcggct
      541 ctcttcgtcc ctcatgtgtc gcgcgatagc aaaaatcccg cagctcaggt catgccttgg
      601 tattcccagg agaaatacct ggatagcttc tctacacatt ccggccacga gtcggcctcg
      661 gactcagcaa cgaccttcgc cagccaactc caaacaacat cgaaatcgta ggcccgaatt
      721 atcaacatcc gtcccaccgc gacaatccca tccttgtcat cgagagtagc caatccggct
      781 agtgtcgcaa gccttacgtg gaaagtatcc gccttttccc cggtttcggc ccgatatcga
      841 catacagttc ccctgcgaca gatgcgggat cggcgagctg ccacgtccgg agattggggt
      901 gcccccagat accaaccgac cgaatagtca gcttcatttg tcccacttcg agtgaacgtg
      961 gacgttcgat gtctgctgct tgtcgaacca gtctgcacgt gtgttgggcc gctcgtgaaa
     1021 gttggcttgc ttgccagtcc tcgcgaatgg gctcgccttg gcagtgggct cgcgatacag
     1081 gcgcacctgg cgctcgacga gttttcgatg gcgccttcgc acgtttcgac caagtggggc
     1141 ttgagctgga gcacggtgcg gcgtgccgag ctcgatgcga tcgaccgctg ggacagggcc
     1201 cacccgccag tgccgctcgt catggcgggc gtcgatggga agtggctcgg ccggcggcac
     1261 aagcgcaagg aaaagttcgt cacgatcgtg agcaacctcg agaccggcgt gcccgtctgg
     1321 atcggctacg gccgcgacgg cgcgacgctc aagagcttcc tcggttcgct cacgcccgag
     1381 cagaaggcgg cgctcaagct cttcgccgtg gacatgcacg agccctccaa gaaggccatc
     1441 cacgaagacc atgcgctcgc ccatgccgcg attgtccacg acccgttcca cgtcatcaag
     1501 cgcgccggcg aggcggtcac ggagcttcgg cgcgcggtat tcttcagggc cggcccggag
     1561 ctgcgcgagg tcggcagggg ctcgcgctgg ctcgtgctgc gagcctggga gaagtccacc
     1621 gacgagcaac agagccgcct tcgccgcttg ttccggctca atggcaagct gtcagagctc
     1681 ttgacggaga ggccgaccga gcgagtcgcg ctgccacttt gccgtggcat ggtcgaggtg
     1741 ttcgcagttc ggtcgaaacg gccagccttt ctcgaccgcg tacacgaagt caccctcgga
     1801 aagtccgccg gaggactccg gctcggaacg gccgctggcg agcgggttga acttgagcac
     1861 cgccccgagc gcgcgctcgc cgagttgatt cttgggaccg tacatcgatg ctccttattt
     1921 acggctcggc ttcgaggagt agccccagtc ttcgggacaa gccttttgcg tgactatctc
     1981 aacgtagttg gcgtattctg gtcggagcag ccgatcgaac gtgaaatctt cagctgccaa
     2041 ggcggcggcg cagctcgtcc atcgccgcgc gccatgcggt cgcgtcagtt tgctcccaca
     2101 gctggcggag ctcggagttt tccatgagga tgcggtcgag cgcggacctt gccctggcaa
     2161 cgagttccgg cgcgaccgcc aacggatgcg cggcgaccca cccatcgacc ttgcgcgtat
     2221 aggcgtcccg ataaccaatt tgtccgcgca agcgggcgat gacttcgcac gccgcgagcg
     2281 caacgcaggc aacctcctga tccaagtatc cgt
//
LOCUS       DASTKU010000588        10500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1230272, whole genome shotgun sequence.
ACCESSION   DASTKU010000588 DASTKU010000000
VERSION     DASTKU010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230272"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /gene="cysS"
                     /locus_tag="VFQ61_20590"
     CDS             complement(<1..921)
                     /gene="cysS"
                     /locus_tag="VFQ61_20590"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET9956912.1"
                     /translation="MPLYLHNTLTKQKELFEPAHPKVVRIYTCGLTTYAPMHIGHART
                     YCFWDLFRRYLEYSGRHVVSVINYTDVDDRIIDRAQGSEGPIDVAERNIASFRRDCRK
                     LHIKDYSAYTRATDFVGEQLAMIEALQKGGHAYTVDGEVLYQVETFEHYGQLSGNTLA
                     AQQVGASGRVEEDVSRKRNPADFSLWKPAKAGEPSWPAERAGWESGRPGWHIECSAMS
                     TTLFGPHFDVHGGGVDNLFPHHENEIAQSEPLCGRPWVKYWLHPEHLDLKDMKMSKSL
                     GNVIGIEELLSRHTYDEVRWFYAMQHYRSKL"
     gene            complement(1032..2798)
                     /locus_tag="VFQ61_20595"
     CDS             complement(1032..2798)
                     /locus_tag="VFQ61_20595"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9956913.1"
                     /translation="MGLTHADEASGARRHYRPLLELGRGGMAKVYLAESLASGLRKLV
                     VLKILNRDLADDAEIRAGFQREAELSARMNHPNVVQVFEVVEFGGVPVIVMEHLDGLA
                     LSRLLQHTKLPLRLHLYVLTQVLAGLSHFHELKDIEGKDFEAVHRDVSPQNVLVLYEG
                     PVKVVDFGIAKVRLAPDQQTRTGLIKGKIHYMPPEQLLGESNLDRRADVFAVGVMLWE
                     AIAGRRMWEGIPEATIMRKLVGGELPKLREAVPDVSPILERITERALAPDRTQRYESA
                     EAMLLDLEHALHDLEGVVRPRELTEFMLKHFGDRRQFQQRTIERALREPASSMSGVME
                     CVTPPPVELERASQAFAQAGDDEPLPSSTFLRGPGSARGPGSSRIFVPTAVSQAAPRR
                     RSHLWLPFLGALLLGGAIGGVLLFRDSAAKRATSVAAPTQADASRSQVRLQVLARPEQ
                     AEILFDGVRLGVGHVEQSLAPREQPATLEVRLPGYATERRSIRLKGDTSVDILLQPVP
                     VDSPGARSATPPVESERKSVSSGAGHARSAPVSPRARPGTSDKPEAPPPAPASAPTTK
                     EGCNPPYRLGPDGVKTYKAECF"
     gene            3311..5176
                     /locus_tag="VFQ61_20600"
     CDS             3311..5176
                     /locus_tag="VFQ61_20600"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET9956914.1"
                     /translation="MLRTALIVSTACLCFSFACVSTNDDLLDSTSGGRGGATGGKGGT
                     GGSRGGSTSRGGSTHAAGAAGEDTGTGGDAPNSSGGSENQAGSTATAGGKASFGGSAN
                     GGVTSNEGGSNNLGGATEDAGAGGEPSSGGSSGGSEPGSGGAANGGTTSSPSGGTANG
                     GTTSSPSGGTPSAGSGGTPVDTSGLVVRYAFDEDSGTTAQDATGHFSAANLVDGAAWA
                     PGRGNGAAVELSAATAHVELPAGLFAGVEKATLATWVNVAAYNPWSRIFDINAGNTKF
                     VFLASDRGPGLGDGPFFAIHTGVPAEDGNIGSAGDLPLGVWKHVALTVDNTTYAMFVD
                     GYPVASTVTNPRYPAEIEPSVAGWLGKSTFDVDAYFKGKLDDFRIYDKVLTQAQIADL
                     AWPDADYTYLRLDETSGSALADSSDRNFDGSLVNGGSFVPGRLGGAVQLTAASDQYIS
                     LPPRVIADCDDVTIALWAKLNSAPPYVRLIDIDGLQNGFIFFTPTAQAPDAQGTGSHP
                     ELRFNIYRTGQSPSDQMVSAPYPEGTVLAGAWHHFAVTLSGTTARLYFDGREIGSNPT
                     MSFKPSQIGIAASGAHAWIGRSTFFNADPELDGAVDEVRISCRAFTSDEIAQLAH"
     gene            5185..6270
                     /locus_tag="VFQ61_20605"
     CDS             5185..6270
                     /locus_tag="VFQ61_20605"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET9956915.1"
                     /translation="MKTSKASVSRAALGGALFAAACSLTLPEEREFFGSEPGGGGGSG
                     TSNANQGGAATAGGTSGTSSGGNPGQAGINSSAGAGGAEGGESGSGGSGAATAGAAGD
                     TSGSGGISGSSGGAGGGAGDENGGAAGSSAPLAPFDPKQGLLAHYAFEETAGTTARDR
                     SPRGNDGLITGSVTWVSTGKVGRALQLSGQQAYVSLPSTFMTNLDEVTLAIWFNQDTR
                     VLWTRVMDFGSSAMHWMYFAPATVQVNEQGARAALDVANFITAEMHMATVLPGPGEWM
                     HVALTWKKTHFACYINGELVAEDTAPMYSPGEFVALTPASETWRAWLGRSSFAVDPYF
                     AGKLDEFRAYDRALAPEHVAALYALKE"
     gene            6276..7205
                     /locus_tag="VFQ61_20610"
     CDS             6276..7205
                     /locus_tag="VFQ61_20610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956916.1"
                     /translation="MTLFPSGTLVSPWRRAGLLAIGAASFGLLPRTARADAGTCVGLH
                     AAGQREVKAGELRKAVKDFMACGSDDSCPAAIRTECMELYASSDRTLPTVIFSVVDDQ
                     GNDVAQVRVFSGAELLTESLDGRALPMDPGKHQLRFELPWGETQNQELLIREGEKNRV
                     VNLKVRDPRQPERAAAPEASATPLETATTHPTYAPKPLPLGFWITSGVGIAALGTGAV
                     FELLGRTKHADLADCSPRCPASRRDEYDTIKQNYLIGDIALGAGVAALGAATIIYFTS
                     HGRTESSARRWPMGVALVPRGQGATLHVTGVRF"
     gene            7305..8717
                     /locus_tag="VFQ61_20615"
     CDS             7305..8717
                     /locus_tag="VFQ61_20615"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HET9956917.1"
                     /translation="MMHGRRRPLLLALFTTLLPVSCIELNSEPAGEEPLGFGGSNGGT
                     PITAAGESGAGGTMTTTSPSLSGSGGQDPSQAGERNHGGMEQAGDSGAGNSNIGDGGV
                     SAAGGTMSTPQGGAADGGAATLSGGASGTEGLGGESHAGAAGGLHVDFSGGGPLDPND
                     RCGVAEYSPDAPPLALPLGGNLDVHDPAIIAVDNQNFVFHTGPGIPIKTSSDLRTWSS
                     AGRVFFGNPSWISALVPGASDLWAPDISHFGGVYHLYYAASTFGSNRSCIGHATRDSL
                     GSGGWLDHGPVICSNAAGTTDDWNAIDPNVVLDEASVPWLTFGSFWSGIKAVRLDSSG
                     VRADNSLYSLAARPTEGKAIEAPFITRRCGYYYLFVSFDYCCRGSSSTYRVLVGRATN
                     VLGPYVDRNGRALLEGGGEAVVSGDSRWHGPGHNAMLFVGDRAYNVYHAYDTLNQGKP
                     SLRIAQLAWDLEGWPVSGGP"
     gene            8767..9885
                     /locus_tag="VFQ61_20620"
     CDS             8767..9885
                     /locus_tag="VFQ61_20620"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HET9956918.1"
                     /translation="MAFISNQAIRVSNWLCLVLAWGAWSCSERQAAPELAAISAELTT
                     VSGPSADVTATETTSTTSSVTVLDLTGDVRAHDPALTRHGAYGDWYVFSTGDAAVGGG
                     AIQIRRSTDLRNWTFAGTVFDAIPAWVSAAVPGVTNLWAPEITYRAGLYYLYYSGSTF
                     GSNNSVIGLATNTTLNPSDPNYQWVDQGMVFRSSPANDYNAIDPAFIQNTDGTPWLAF
                     GSFWSGIRMIRLEWPSGKAAPSSTEPLRLADRFVPPNAIEAPYILYRGGWFYLFVSLD
                     FCCQGTNSTYKIAVGRSRQATGPYFDRLGTPMAHGGGTVILSERGDMFGPGGQSLFGG
                     YMAHHWYDAANNGEIRLGIRRLAWDLSGWPKAATDWVQ"
     gene            complement(9901..10335)
                     /locus_tag="VFQ61_20625"
     CDS             complement(9901..10335)
                     /locus_tag="VFQ61_20625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HET9956919.1"
                     /translation="MPIWFDGFPLEIVRERSQNTLVGHLGIEFLEAGDDYVRARMPVD
                     DRTRQPAGMLHGGASVVLAETLASWAASFTVDPQKNHCVGLEINANHVRALASGWVIG
                     TARPAHLGRSTQIWEVRMVDESNRLICISRVTMAVLSTPSRY"
BASE COUNT         1913 a         3298 c         3364 g         1925 t
ORIGIN      
        1 gagcttggag cgatagtgct gcatggcgta aaaccagcgc acctcgtcgt aggtgtggcg
       61 tgacaagagc tcctcgatgc cgatcacgtt gccgaggctc ttggacatct tcatgtcttt
      121 gagatcgagg tgctcggggt ggagccaata cttcacccag ggcctgccgc acagcggctc
      181 gctctgcgca atttcgttct cgtgatgggg gaacaggttg tcgacgccgc cgccgtgcac
      241 gtcgaaatgc ggtccgaaca aggtggtact catggccgag cactcgatgt gccaaccggg
      301 gcgaccgctc tcccagcccg cgcgctcggc cggccagctc ggctccccgg ccttggctgg
      361 cttccagagg ctgaaatcgg cggggttgcg cttgcgcgag acgtcctctt ccacgcggcc
      421 gctagcgccg acttgctggg cggcgagcgt attgccggag agctggccgt agtgttcgaa
      481 ggtctccact tgatagagca cctcgccgtc cacggtgtat gcgtgcccgc ccttttgcag
      541 ggcctcgatc atggcgagtt gctcgccgac gaagtccgta gcacgcgtgt aagcggagta
      601 gtccttgatg tgcagtttgc ggcagtcacg gcggaagctc gcgatgttgc gctcggcgac
      661 gtcgatgggc ccttcactgc cctgcgcacg gtcgatgata cgatcgtcta cgtccgtgta
      721 gttgatgaca ctgacgacgt gccgaccgct gtactccagg tagcgccgaa acaaatccca
      781 gaagcagtac gtgcgagcgt gcccgatgtg catcggcgcg taagtggtga gcccgcacgt
      841 gtagatgcgg acgaccttcg ggtgcgccgg ctcgaagagc tctttctgct tggtaagggt
      901 gttgtggagg tacaacggca tggccgcttg ctttacactg gctcgcgccc tccgacaccc
      961 ccaaaagcgg cagataaccg ggccgctgcc gaaaccgctc cccctccggc cccgaactcg
     1021 cggttaggca gtcagaagca ctcggccttg taggtcttca cgccgtcggg gccgagccga
     1081 taagggggat tgcatccctc tttggtagtc ggggccgagg caggcgccgg cggtggagcc
     1141 tcgggcttgt ccgatgtgcc gggcctagcc cgcggcgaga caggggccga gcgcgcgtgc
     1201 ccagcgccgc tcgatacgct ctttctttcg ctttccaccg gcggcgtggc tgagcgtgca
     1261 ccgggtgaat caaccggcac gggctgaagc agaatgtcga cggacgtatc acccttcaag
     1321 cgaatcgagc gtcgttccgt ggcgtagccc ggcaatcgca cctcgagcgt tgccggctgc
     1381 tcgcgcggcg ccagactctg ctccacatgg ccaaccccga gccttacacc atcgaacaag
     1441 atctctgctt gttcggggcg ggccaacact tgcagccgca cttggctgcg gctcgcatcg
     1501 gcttgagtcg gggccgccac gctcgtcgcg cgtttggcgg ctgaatctcg aaacaacaga
     1561 actccgccga tcgcaccacc cagcaacagg gcgccgagga acggcagcca gaggtgcgag
     1621 cgccgccgcg gtgctgcctg cgagactgcg gtcggcacga aaatgcggga agaaccgggc
     1681 cctcgcgccg atccgggccc gcgcaggaac gtgctactcg gcagcggctc atcgtcaccc
     1741 gcttgggcga aggcctgact cgcccgttcg agctcgaccg gcggcggagt tacgcactcc
     1801 atgactccgc tcatgctgct ggccggctcg cgcaaggcgc gctcgatggt gcgctgttga
     1861 aactgccgac gatctccgaa gtgcttgagc atgaactcgg tgagctcgcg cggtcgcacg
     1921 accccttcga ggtcgtgcaa cgcgtgttcg aggtccagca gcatggcctc ggcgctctcg
     1981 taacgctgcg tacgatcggg tgccaacgcg cgctcggtga tgcgctcgag gatgggtgat
     2041 acgtcgggca cggcctcacg cagcttcggc aactcacccc cgacgagctt gcgcatgatc
     2101 gtcgcctccg ggatgccctc ccacatgcga cgccccgcga tcgcttccca cagcatcacc
     2161 cccacggcga atacgtctgc tcgccgatcg aggttcgatt ccccgagcag ctgctcgggt
     2221 ggcatgtagt gaatcttgcc cttgattaga ccggtgcggg tttgctggtc cggcgcaagc
     2281 cggaccttcg cgatcccgaa atccacgacc ttcactggcc cctcgtacag caccagtacg
     2341 ttttgagggg acacgtcgcg gtgaacggcc tcgaaatcct taccttcgat gtccttgagt
     2401 tcgtgaaagt gactcagccc ggctaacact tgagtcaaca cgtacaagtg taggcgaagc
     2461 ggcagttttg tgtgttgaag gagccgcgag agggcgagtc cgtccaggtg ttccatcacg
     2521 attaccggaa caccgccgaa ttcgacgact tcgaacacct gtacgacatt ggggtgattc
     2581 atgcgcgcgg agagctccgc ttctcgctga aaacccgcgc ggatctcggc atcgtcggcg
     2641 agatctcgat tcaaaatttt gagcacgacc agcttgcgaa gcccggaggc gaggctctcg
     2701 gccaggtaga ccttcgccat tcctccacgg cccagctcca aaagcggccg gtagtggcgg
     2761 cgagctccgc tcgcctcgtc ggcgtgagtg agtcccatag aaattctggt agacgctacg
     2821 gcgcgcgacg gatttcgcta agaggctgcg cgccaactgc ggtcaggctt cgaatgtact
     2881 gtatcgccga gcactatgag tcaactgtgc agggttgatt cgtgccaatt ttgtacttcg
     2941 cgtagcaaaa tccgactctc atggattcga cttctgctgc tcctcgtcga ccatgcctgc
     3001 gcgcattcga gtgacggtcc attggcactc tcgccggtgc tcgcgaacct aacaccatcg
     3061 gattaaatga caccacgacc gaagtgtgat gcacgagagc atcagcgcgg ttacccgaca
     3121 ttcacaaatc aaggcgtaaa cccgaggtgt cgacaatgga caggggacga caattgacag
     3181 ttatcgatcg agttgatttt cgactggcaa gatgcggcga gttgtcgcat tagggagtga
     3241 tacgggattt gtaaattact cacggtctcg cctgcaatcg aggccgatgt agacgaggat
     3301 aggagtctcc gtgttgcgta cagccttaat cgtaagcacc gcgtgtttgt gtttttcgtt
     3361 cgcttgtgtt tcgaccaacg atgacctgct cgactccact tccggagggc gcggcggcgc
     3421 gacgggcggc aaaggcggca ccggagggag ccgcggcggg tccacgtctc gcggtggcag
     3481 cacgcatgca gccggcgcag cgggtgaaga caccggcacc gggggagacg ctcccaacag
     3541 cagtggaggc agcgaaaacc aagcgggcag cactgcgacc gcgggcggaa aggccagttt
     3601 cggcggctct gccaacggtg gagtcacgag caacgaaggc ggcagcaata acctgggcgg
     3661 cgcgaccgaa gacgcaggag ccgggggcga gcccagttcg ggaggaagct cgggaggaag
     3721 cgagccgggt agcggcggcg ccgccaacgg cggaaccacg agctcgccga gcggcggcac
     3781 ggccaacggc ggaaccacga gctcgccgag cggcggtact cccagcgctg gcagcggcgg
     3841 aactcctgtg gatacctcgg gcctcgtcgt tcgatatgcc ttcgacgagg acagcggcac
     3901 gaccgcccag gacgcgacgg gacacttttc cgcggcgaat ctggtcgacg gtgcagcctg
     3961 ggcgcccggg cgcggcaacg gggccgcggt cgagctgtcg gcggcaactg cacacgtgga
     4021 gttgccggca ggcctgtttg cgggtgtgga aaaggccacc ctcgcgacgt gggtgaatgt
     4081 ggccgcttat aacccttgga gtcgaatatt cgacatcaac gctggcaata caaaatttgt
     4141 attcttggcc tccgatcgcg gtccgggcct gggtgatggc ccattcttcg cgatccatac
     4201 gggagtgccg gcagaagacg gcaacatcgg aagtgcggga gacctgccac tcggcgtctg
     4261 gaaacacgtc gcgctcaccg tggataacac gacatatgcg atgttcgtgg acggctaccc
     4321 ggtcgcaagc accgtaacaa acccgcgtta tcctgccgaa atcgagccca gcgtggcggg
     4381 ttggctcggc aaatcgacgt tcgacgtgga tgcctacttc aagggcaaac tcgatgattt
     4441 ccgaatttac gacaaggtgt tgacgcaggc acagattgcc gatctggctt ggcccgacgc
     4501 ggactatacg tatcttcgtt tggacgagac gagcggctcc gccctggccg actcttcgga
     4561 ccgcaatttc gacggcagct tggtcaacgg tggcagcttc gtgccggggc gcctcggtgg
     4621 cgccgtccaa ctcaccgccg catccgatca gtacatttcg ttaccgccgc gcgtgattgc
     4681 ggattgtgat gacgtgacga tcgcgctctg ggcaaagctc aactcggcgc caccttacgt
     4741 ccgcctaatc gatatcgacg gtctgcagaa cggattcatc ttcttcactc ccaccgccca
     4801 agcgccggat gcgcaaggca ccggctcgca tcccgagcta cgattcaata tctatcgtac
     4861 cggtcagagc ccctcggatc agatggtcag cgccccgtac ccggaaggta cggtgctcgc
     4921 gggcgcatgg catcatttcg cagtcacgct gagcggcacc accgcgcggc tctatttcga
     4981 cggaagagaa atcggcagca acccaacgat gtccttcaag ccctctcaga tcggcatcgc
     5041 ggcaagcggg gcacacgcct ggatcggtcg atctacgttc ttcaacgcag atcccgagct
     5101 cgatggcgcg gtcgacgagg tgcgcatctc ctgtcgcgcc ttcacgagcg atgagatcgc
     5161 gcagctagcg cattgagtgt cagcatgaaa acttcgaagg cctcggtttc gcgggcggcg
     5221 ctcggcggcg cgttgttcgc cgccgcatgc tccctcacgc taccggaaga acgagagttc
     5281 tttggcagcg agcctggcgg aggcgggggg agtgggacat cgaacgccaa tcaaggtggc
     5341 gcggcgacgg ccggcggaac gagtggaacc tctagcggcg ggaacccggg ccaggcagga
     5401 atcaactcga gcgcgggcgc gggcggcgca gaaggcggtg agtcggggtc gggcggcagc
     5461 ggcgcggcga cggccggcgc ggcgggggac acctcgggat caggcggaat ttcgggcagc
     5521 tcgggggggg ctggcggtgg agcgggcgat gagaacggcg gggcggcggg ctccagcgcc
     5581 ccgctcgcgc cgttcgatcc caagcaaggg ctgctcgccc actacgcctt cgaagagacc
     5641 gccgggacca ccgcgcggga ccgcagtccg cgcggcaacg acggcctgat tacgggctcg
     5701 gttacctggg tgagcacggg caaagtgggc cgagccctgc agctctcggg gcagcaggcg
     5761 tacgtttccc tgccttccac gttcatgacg aacctggacg aggtgacgct cgcgatctgg
     5821 ttcaatcaag atacgcgcgt gctgtggacg cgggtgatgg atttcggctc gtccgcgatg
     5881 cactggatgt acttcgctcc cgccaccgtc caagtcaacg agcagggcgc gcgcgcggcg
     5941 ctggatgtcg cgaacttcat caccgccgag atgcacatgg ctactgtgtt gcccgggcca
     6001 ggcgaatgga tgcatgtcgc gttgacgtgg aagaagacgc acttcgcttg ttacatcaac
     6061 ggcgagctcg tggctgaaga cacggcaccc atgtacagcc cgggcgagtt cgtggcgctg
     6121 actccggcga gcgagacctg gcgcgcctgg ttgggccgat cgagcttcgc ggtggaccct
     6181 tatttcgccg gcaagctcga tgagttcagg gcctacgatc gggcgctcgc tccagagcat
     6241 gtcgccgcac tgtacgcact caaagaatag agatcatgac tctcttccca tctggcactt
     6301 tggtttctcc ttggcggcgc gccgggcttc tcgcgatcgg cgccgcctcg ttcgggctcc
     6361 taccccgaac cgcacgagcc gatgcgggca cgtgtgtcgg cttgcacgca gccgggcagc
     6421 gcgaggtcaa ggcgggcgaa ctgcgcaagg cggtcaaaga cttcatggcc tgcggctccg
     6481 atgactcctg tcccgcggcg atccgcaccg agtgcatgga gctctacgct tcgagcgatc
     6541 gcacgttgcc gaccgtcatc ttctcggtgg tcgacgacca aggcaacgac gtcgctcaag
     6601 tgcgggtgtt ctccggagcg gagctgctga ccgagtcgct ggacggtcgc gcgctgccca
     6661 tggatccggg caagcatcag ctccgcttcg agctaccctg gggcgagacg cagaatcaag
     6721 agcttctaat ccgcgaaggc gagaagaacc gagtcgtcaa tctgaaggtt cgggacccac
     6781 ggcagcccga acgagccgcg gctccagagg cgtcggctac gccgttggag accgccacca
     6841 cccaccccac ctacgcgccg aaaccgctac ctctgggctt ctggatcacg agcggtgtgg
     6901 gcattgcggc gctcggtact ggcgccgtgt tcgaactgct gggacgcacc aagcacgcgg
     6961 atctcgcgga ctgctcgcca cgctgtcccg cctcgcgtcg agacgaatac gacaccatca
     7021 agcagaacta cttgattggc gacattgctc tgggcgcggg tgtcgcagcg ctcggagccg
     7081 ccacgatcat ttacttcaca tcccacggcc gcaccgaaag ttccgcccgt cgttggccga
     7141 tgggggtcgc gctcgtgccg cgaggccagg gcgcaacgct gcacgtgacg ggtgttcgct
     7201 tctgagtctg catatacaga cggcatccca ggcaattcag ggggaactgg gtcgttggag
     7261 cgagtgatga atctcgatct atcgagttcg ccgctggggc actcatgatg cacggccgac
     7321 gcagaccact cttgctcgca ctgttcacca cgctactgcc ggtgtcttgc atcgagctca
     7381 actcggaacc cgcaggcgaa gaaccgctcg gcttcggagg cagcaatgga ggaaccccga
     7441 tcactgccgc gggtgaatcc ggtgcgggag gcacgatgac caccacgtcg ccgagcctct
     7501 ccggtagcgg tggtcaggat ccctcgcagg ccggcgaacg aaaccacggt ggtatggagc
     7561 aagccggtga ttcgggcgca ggcaactcga acatcggtga cgggggcgtg agcgccgcag
     7621 gcgggacaat gagcacgccc caaggtggag ccgcagacgg cggcgcggca acgctctccg
     7681 gcggtgcttc cggcaccgag ggtttaggtg gtgagtcgca cgcgggcgcc gcgggcggtc
     7741 ttcacgtcga tttcagcggc ggcggccctt tggatccgaa cgatcgctgc ggtgtggccg
     7801 agtactcgcc cgacgccccg ccgctggcgc tgcccttggg gggcaacctc gacgttcacg
     7861 atccagccat catcgccgtc gacaaccaga acttcgtatt tcataccggg cccgggattc
     7921 cgatcaagac ctcctcggat ctcagaacct ggagcagcgc ggggcgcgta ttctttggca
     7981 atcccagctg gatttctgcg ctcgtgccgg gtgcttcgga cttgtgggct cccgatatct
     8041 cgcatttcgg cggcgtttac cacttgtatt acgcggcctc gacgttcggg agtaaccgct
     8101 cgtgcattgg gcacgcgacc cgagattcac tcggctctgg cggctggctc gatcacggcc
     8161 cggtgatttg ctcgaacgcc gcgggcacga cggatgactg gaacgccatc gaccccaacg
     8221 tggtcctgga cgaggcttcc gtgccctggc ttacgttcgg cagcttctgg agcggaatca
     8281 aggccgtccg cttggattcg agcggagttc gggcggataa ctcgctctac tcactcgcag
     8341 cgcgacctac cgaaggcaaa gccatcgaag cgccgttcat cacccgacgc tgtggctact
     8401 attatctgtt cgtatcgttc gactactgct gtcgcggttc ttcgagcacc tatcgagtac
     8461 tcgtgggccg tgccacgaac gtgctcggac cgtacgtcga tcggaatggg cgcgcgctac
     8521 tggaaggcgg aggcgaggcc gtggtctccg gcgactcacg ttggcatggt cccggccaca
     8581 acgcgatgtt gttcgtcggg gatcgtgcgt acaacgtcta tcacgcctat gataccctga
     8641 atcagggaaa gcccagcttg cgcatcgcgc agctagcgtg ggatctcgag ggctggcctg
     8701 tttcaggcgg accttgaaga tgcgcggcga ggcgtaggac tcgctttcaa cgtaaggagg
     8761 agtgacatgg cgttcatatc gaatcaggcg atccgtgttt ccaattggct ttgcctcgtc
     8821 ctggcgtggg gagcgtggtc ctgcagcgag aggcaagcag cgcccgagct cgccgcgatt
     8881 tccgcggagc tcacgaccgt gtctggaccg tccgccgacg tcaccgcgac cgaaactacg
     8941 agcaccacgt cgagcgtcac cgtgctcgat ctgaccggtg acgttcgcgc ccacgatcca
     9001 gcgctgacga ggcacggcgc ctacggcgat tggtacgtgt tttcgaccgg cgacgccgcc
     9061 gtcggcgggg gcgcaatcca gattcggcga tcgacagacc tgcgcaactg gacgttcgcc
     9121 ggcaccgtat tcgacgccat cccggcctgg gttagcgcgg cggtaccggg ggtcacgaat
     9181 ttgtgggcac cagaaattac gtatcgggct ggcttgtatt atctctacta ttccggctcg
     9241 acgttcgggt cgaacaactc ggtcatcggc ttggccacga ataccacgct gaacccaagc
     9301 gatcccaact accagtgggt ggaccagggc atggtgttcc gctcgtcacc ggccaatgac
     9361 tacaacgcca tcgatcctgc gttcatacag aatacggatg gtacaccttg gttggcgttc
     9421 ggctcgttct ggagcggcat acgcatgatc cggctcgaat ggccctcggg caaagccgcc
     9481 cccagcagca cggagccgct gcgtctcgcc gatcgcttcg tgcctccgaa cgcgattgaa
     9541 gccccttaca tcctctatcg cggcggttgg ttctacttgt tcgtctcgct cgacttctgc
     9601 tgccaaggca cgaatagcac ctacaagatc gcggtgggtc gttcacgcca agccaccggc
     9661 ccctacttcg atcggctcgg gacgcccatg gcgcacggcg gaggcaccgt cattctatcg
     9721 gagcgagggg acatgttcgg cccgggtgga caatccctat ttggcggata catggcccat
     9781 cattggtatg atgctgccaa caacggcgag attcgcctgg gaatccgcag gctcgcgtgg
     9841 gacctgagcg gctggcccaa ggccgccacc gattgggttc agtagtcgat ccccggcagc
     9901 tcagtaacgg ctgggcgtgg agagcaccgc catcgtcacg cgcgagatgc agatcagtcg
     9961 gttcgactca tcgaccatgc gcacctccca aatctgagtg gaacgcccga ggtgcgcggg
    10021 gcgagccgta ccgatgaccc aacccgaggc caaggcccgc acatggttcg cgttgatctc
    10081 caagcccacg cagtggttct tctgaggatc gacggtaaag gacgccgccc aagaggccag
    10141 ggtttcagcc agcaccaccg aggccccccc gtgcaacata ccggcgggtt gtcgggtgcg
    10201 gtcgtccact ggcattctgg cgcgcacgta atcgtctccc gcctccagaa actcgatccc
    10261 taggtgaccc accagggtgt tttgactgcg ttcacgcacg atctcgagcg gaaaaccatc
    10321 gaaccaaatc ggcatcggcc tacctcagaa tccaattcaa aactcgatat cacacccgac
    10381 gtgaccgcct ccaggccgac tcgagggccc ttggcggtca cgcccgctct cacttcgcaa
    10441 actctaggtt tcgatccgag cgatcttgat ctgcaagtcc ttggcttcga gcgtgtcgcg
//
LOCUS       DASTKU010000589        12809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_337008, whole genome shotgun sequence.
ACCESSION   DASTKU010000589 DASTKU010000000
VERSION     DASTKU010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337008"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(742..1560)
                     /locus_tag="VFQ61_20630"
     CDS             complement(742..1560)
                     /locus_tag="VFQ61_20630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956920.1"
                     /translation="MKRSGKSGWVGMGLVFSAVSFLMGCGGHTSLGEDAPQGFGGSSG
                     DESVWGGVAGAAQGGGKHNPPVARGGAGGAVGIIAGAGGITAGAGGIIAGAGGTTAGA
                     GGSAGGSAEQVACPCSRRPGANNSVRCPPGIGESASAVIGVEGGSIELTGQQGTGVAA
                     LVTIAPKQLSADQELTLTEVDTPPPAELNDYSPIYTLTGDVLPARISVRLPFGNLDGE
                     VARDLTVYGAETADGPFVALPNIYVNAGFVEGDVDGYSAFLVAGARGPELQNCP"
     gene            complement(1605..2384)
                     /locus_tag="VFQ61_20635"
     CDS             complement(1605..2384)
                     /locus_tag="VFQ61_20635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956921.1"
                     /translation="MKGPARLRSLSELPSGLSSALDAAPSGPSEAELLSLAAKVGGAV
                     GVPLAAPPALVIAGKSTTSVLAAKPLGSVLAWMLGGMVLGAGLSGASMSWTSTSDKPA
                     PAVRVKSPRAPRVIPPAVIEQPSDTEPEAPRPVPATARALSRGPEVPVVETPALVAPP
                     EPEVRSELELLREARRALERDPARTLELCAEHARSHAIGVLEQERDVLQIDALLRLGR
                     LPEARARFESFERTYPHSAHTPRLRGQLEEKSTANATRTSK"
     gene            complement(2381..2914)
                     /locus_tag="VFQ61_20640"
     CDS             complement(2381..2914)
                     /locus_tag="VFQ61_20640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9956922.1"
                     /translation="MFALKQDKNHEPLEFSDVFAHYSPFAWRVLARLGVERRDVPDAC
                     QEVFLVVHRRLPEFDASRANLRSWLYGICVRTASDYRRRHPSRRETNDDALHSLGVPA
                     TQEHSVDEARAWKRLARVLDRLDSAQRSAFVLYELEAISMNEVAAILECPLQTAYSRL
                     HAARRAVFEAFAVQEAR"
     gene            complement(2989..4101)
                     /locus_tag="VFQ61_20645"
     CDS             complement(2989..4101)
                     /locus_tag="VFQ61_20645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956923.1"
                     /translation="MTRRSSLTHARRNSSTWGLALGVTLLVTGHVSAQGRGAPSALNL
                     VRLAGAESCPDLRALSRQVDAHLGRGAFVSPSDAEVLFDVVLTVGERGGWSAMIVAST
                     VSGEQLGQRQLLLPQPNCAEASDAVALALALMIEPNLVGAEPAPTPVPRSAPSVGPAP
                     PPDSAPKASAPVGVTAPAVSRTSPRASRVSPPAPRPWRTRVAVGALGTLGQVPQAAEG
                     LFVGARFAPPHGSLSFELGASLVAAKRAELRDGAGGDISATLGEALAHWSIAESSGAA
                     IAVSPKLQVGRMSAVGSGFSGTSRAASALLLNVGIALEAAVALNRNFSLLVRPSLVFP
                     LFRESFEATRSDGRTETIFQPAAAIAGASLGIGFQP"
     gene            4713..5339
                     /locus_tag="VFQ61_20650"
     CDS             4713..5339
                     /locus_tag="VFQ61_20650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9956924.1"
                     /translation="MPLIAALACQPAVNQPAQQPARTAIAIGETCTPDGKSPPEILDE
                     NLQPIDLTNELSIEAAVGHRVVLCAPVRRELRGRVVDENDQPVAGATVIVESWQTPPP
                     IGGLEQRRRLLRTTKTETMASGAWHVPAESAWMLGILAADGFPFFVSSYCVLAVGFAP
                     FVFDPWQHPHANAGSSVGDIVLRESEPRNIPALGAETSSCGIALEPSL"
     gene            5736..5951
                     /locus_tag="VFQ61_20655"
     CDS             5736..5951
                     /locus_tag="VFQ61_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956925.1"
                     /translation="MGADRGGPRRRLRADSLCEQFSEPSGHYKLALIHVSGALAQFPP
                     PTRAIGVRNPLSRGEKITLKNGADKIT"
     gene            complement(5944..6576)
                     /locus_tag="VFQ61_20660"
     CDS             complement(5944..6576)
                     /locus_tag="VFQ61_20660"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HET9956926.1"
                     /translation="MLERRHRPVDRDPVRALSSTSRCQGPSYLDSAKVAAVPLSAGDI
                     RTALDALSEELESATERAELLVVGGAALVLLYGARQATKDVDACFLKPSAAVVRSAAA
                     RVAGSLDLPEDWLNDGAKAYIVTLTEGPILYESESLLVRAPSIAQLLAMKLAAWRDEI
                     DRSDARLLLSNVQGSLEDVRALIEPLVLPHQRDKAAYALDDLWESIHGTT"
     gene            6902..7534
                     /locus_tag="VFQ61_20665"
     CDS             6902..7534
                     /locus_tag="VFQ61_20665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9956927.1"
                     /translation="MDQEQTEIWEAVFADKQTMWGLEPTRSAILAKDYFLRMGVRNVL
                     IPGVGYGRNAKLFCDHGMSVTGIEISETAISLARSRLGLDIPIHHGSVANMPFDTRQY
                     DGVFCYGLIYLLDTDGRAKLIRDCWEQLAPGGSMIFTVISKEAPMYGQGQRLGQDWYE
                     IHPGMKMYFYDADSVEREFGDHGLVDHSKIDEPLHNGSSFPFINVVCRKR"
     gene            complement(7724..8599)
                     /locus_tag="VFQ61_20670"
     CDS             complement(7724..8599)
                     /locus_tag="VFQ61_20670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9956928.1"
                     /translation="MKPVSDDQIHEVADTAIWVAGYRAMESERPDALFRDPLAGRLAG
                     ERGRTIALTMAGARQFGWTIVVRTSVIDSLIHEAIAKGFDAVLNLGAGLDTRPYRLEL
                     PSGFQWFEVDHPKLIELKNHQLAGEVASCALERISADLTNASERTGLLDQIAKRAEKI
                     LVLTEGVIPYLSQEEVAALARDLGAHASYRAWIADYSSPFLNRQMRRQRDLRKRLRNA
                     PRRFSPIDWEGFFQEQGWRLTEMRYLSVEGKRRGRPAPLPWWFQALNMLAFRRFDGEV
                     ERMLGYGLLEPVREI"
     gene            complement(9020..10006)
                     /locus_tag="VFQ61_20675"
     CDS             complement(9020..10006)
                     /locus_tag="VFQ61_20675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956929.1"
                     /translation="MLHKRVPLIWAFAGGGLSGAAILVLLQSEADRNQSSTPVVDARA
                     MILDCLGRLNSVSADEHAPEGAATQAKSALTPSVVSVPTNSAIPSGTGVLLPSVAGGT
                     EAADTEPTNEVGSSVAAVLARLESDYRRGTALVASSDAASPNDRPSSAGGSSAPAGAD
                     KTATLQPQGAAAPRDVALANTPPPAESPTTREASVALSAPSPSNQQQTQLDPTADSKP
                     EAEVVLASRNSDRGSVVYMGNVQQNIHVGDTYQGAALAAQQMAVLQQQLAMIQYMQLL
                     TLASRFPRAVPPISVNRPTVPISSRHVTAFPSSITNTDNPWGFDFPPTVLVR"
     gene            complement(10179..11057)
                     /locus_tag="VFQ61_20680"
     CDS             complement(10179..11057)
                     /locus_tag="VFQ61_20680"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9956930.1"
                     /translation="MSYYLERIQHGVDYIEARLHDDLEIARIAKEAGLSQWHFQRIFK
                     SLTGETLKTYIRSRRFAKALELLSSTQLRVLDIALLSGFESQEAFARAFKKAFGMTAQ
                     DYRKLGKRNVFVKKPQFDADYLTHIHHNVSLEPEVRFQKQMNLVGMRTQFFGTDSEKN
                     NIGARLPPLWKAFLDRLPEIDGRVPGVCYGVVRQEQHDSDRLEYHAAVEVTEAAHPPK
                     GMVSFQVPAASYARFEHRGPAQQIDHTVSYAYSTWLAQSEKGYRHTGGPDLEIYGAAY
                     HPTAATSVMHYALPIT"
     gene            complement(11061..11423)
                     /locus_tag="VFQ61_20685"
     CDS             complement(11061..11423)
                     /locus_tag="VFQ61_20685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956931.1"
                     /translation="MSASYRATWETYVEAWKVSSSESKRQLFGRCLVKRCVYTDPLTR
                     TESWDELLQYMVAFHQQVPGGHFVTEQFISHHGKSMARWTMRNGEGSVIGDGISYGEY
                     DDEGRLTSMTGFFETPEN"
     gene            11930..12151
                     /locus_tag="VFQ61_20690"
     CDS             11930..12151
                     /locus_tag="VFQ61_20690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956932.1"
                     /translation="MTWYYRTHGAGVDIDRGVVCGGIDFDFDKIEPDPWRLRIFVEKQ
                     VNAGTLNAEYAELVQDEDRFDAAARELLG"
     gene            complement(12245..12622)
                     /locus_tag="VFQ61_20695"
     CDS             complement(12245..12622)
                     /locus_tag="VFQ61_20695"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9956933.1"
                     /translation="MPRVSICVDVPDLKAATTFYCQALGCSLDKEQASHNTLLVGDTK
                     LHLLLKEGGTEATATASCTRTYERHWTPVHLDFAVDDVDAAVAEVTRLGGTVEGLQRG
                     DWGAAAFCADPFGNGFCLLAIRA"
     gene            12621..>12809
                     /locus_tag="VFQ61_20700"
     CDS             12621..>12809
                     /locus_tag="VFQ61_20700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956934.1"
                     /translation="MRSAYQLSPVASRIVEDVNIRPSGPSPCASAARGKWRLYQNAGT
                     LVGSETWFHWAPTWAASPA"
BASE COUNT         2370 a         4018 c         3994 g         2427 t
ORIGIN      
        1 ccaggttcga tcggagagct ctttcgtcac gcgctccggt gcgccaacga cacgcagaat
       61 cacgatcgtc tcgggccaat gcgtcatgca cgcgaactcc tggctcaact tactgacccg
      121 cgacccaaag cgtagctacg ctcccgcagc ctcgtgtgca gcgtcgcttc aggtcggctt
      181 tccgtatccg cgtccccaat gcttgcgccg cccctgcgtc accaccccaa cccataaagc
      241 cgcccaagca agattcggaa tcatcctcga cgggtatgct aggcagctcc ttgcggcgcg
      301 gcctaaggcc caccttcctc tcgaaactac cgattcgcat ggctgaggct caagaccgag
      361 cggaccacaa aacaaagccc gctgagccgc tgcgggtgcg gctcgaaaag cccgagcacg
      421 cgtggctctg atgacgcggg ctccctgttt gacgactgac gaaaccctcg ctttcgcttt
      481 catggcggag tccgtgccca tgcgcatcgc tctcggttct gatcacgccg gcttcgcgct
      541 aataaagcgg gtcaaggcct tacttgaaga gctagtcacg aggcagatca atgagcccag
      601 cgccgcgact agtcgaccct tggcttagcc tcacccggga cactcgtggc cacggcgccg
      661 tggccacgag tgtccgactg tctcaaccgc gcatcacccg agtgtctctt caggcaaccc
      721 ggagttgtgg attacgaagt ctcacggaca attttggagc tcgggcccgc gcgcccctgc
      781 aaccaggaac gctgaatagc cgtcgacgtc accttcgaca aaaccggcgt tcacatagat
      841 gttgggcagc gcaacgaagg gcccgtctgc tgtctctgcg ccgtatacgg tgaggtcgcg
      901 cgcgacttcg ccgtcgaggt tcccgaatgg tagccggacg ctgatgcggg ccggtagaac
      961 gtcaccggtc agcgtataga ttggcgagta gtcattcagc tcggccggag gcggcgtatc
     1021 gacctcggtc aacgtgagct cttgatctgc gctgagttgc ttcggcgcga tcgtgacaag
     1081 cgcggccact cccgtgcctt gttgccccgt caactctatc gagccgcctt caacaccaat
     1141 gacggcgctc gcgctttcgc cgatgccggg cgggcagcgc acgctgttgt tggcgccggg
     1201 gcgccgcgag caggggcacg ctacctgttc cgccgaacca cccgcactgc cgccggctcc
     1261 agcggtagtg ccaccggctc cagcgataat gccgccagct ccagcggtaa tgccgccggc
     1321 tccagcgata ataccgaccg ctccacctgc accgccgcga gcaactggcg ggttgtgttt
     1381 gccaccccct tgcgcggcgc cggctacacc gccccaaacg ctctcgtcac cggagctccc
     1441 tccgaaaccc tgcggtgcgt cctctcccaa gctggtgtga cctccgcaac ccatgagaaa
     1501 gctgaccgcg gaaaacacca accccatccc cacccaacct gacttgccac ttcgcttcat
     1561 tgtttgttcc tccgacttcg aggatgattg gcggtagagg gtgttcattt tgacgtgcgc
     1621 gtcgcgttag ccgtcgattt ttcttcgagc tgccctcgca acctcggcgt gtgcgccgag
     1681 tgcggatagg tccgctcgaa gctctcgaag cgagcccgag cttccggaag acgcccgagc
     1741 cgcagcaggg catcgatctg cagcacgtca cgttcctgct ccagcacgcc tatagcatga
     1801 gaccgagcat gctcggcgca gagctcgagc gtacgcgcgg ggtcgcgctc gagtgcgcgg
     1861 cgggcctcgc ggagcagctc gagctcgctc cgcacttcgg gctccggggg cgcaacgaga
     1921 gccggggtct cgaccacggg cacctcaggg cctcgcgaga gagcgcgcgc agtggcgggg
     1981 acaggccttg gggcttcggg ctcggtgtcg ctcggctgct cgatcacagc aggcggtatc
     2041 acgcgcggcg cgcggggcga cttgacccgg actgcaggag cgggcttgtc ggaggtcgac
     2101 gtccaactca tcgacgcccc cgacaagccc gctcccagca ccattccacc taacatccac
     2161 gccagtactg accccagcgg cttcgccgcc aaaaccgaag tcgtagactt gcccgcaata
     2221 acgagcgcgg gcggcgctgc caaaggcaca cctacggcac ccccgacctt cgccgccaag
     2281 gacagaagct cggcctcgct cgggccactg ggcgccgcgt cgagcgcgga actcagtccg
     2341 ctcggtaact ccgacagcga gcgcaaacgc gccggcccct tcatcgggcc tcctgcacgg
     2401 cgaacgcctc gaaaactgca cggcgcgcag cgtgaaggcg agagtaggcc gtctgcagcg
     2461 ggcactccag aatagcggcc acttcgttca tcgagatcgc ctcgagctcg tacagtacga
     2521 acgcgctacg ttgcgccgag tccagccgat cgagcacgcg tgcgagccgc ttccacgcgc
     2581 gcgcttcgtc cacactgtgc tcctgcgtgg ccggtacgcc caggctatgc aacgcatcgt
     2641 cgttcgtttc gcgccggctg gggtgtcggc gacggtagtc actcgccgtg cgcacgcaga
     2701 tgccatacag ccaactccgc aggttggcgc gcgatgcatc gaactcgggc aatcgccggt
     2761 gcaccaccaa gaacacctct tggcaggcgt ccggaacgtc gcgacgctcg acgcccaggc
     2821 gggccagcac cctccacgca aacggcgagt agtgagcgaa cacatcgctg aattccagtg
     2881 gttcgtgatt tttgtcctgc ttcaaagcga acaaggtttt tctcggcgct tctaggcgca
     2941 ttggcaagca cgcgcgcggt ttatgaagct cctcaaagaa aacgaaactt acggctggaa
     3001 gccaatgccc aagctggcgc cggcgatggc tgcggcgggt tgaaagatcg tctcggtccg
     3061 accatcgctc ctcgttgctt cgaacgactc acgaaagagc ggaaaaacta aacttgggcg
     3121 cacgagtagg ctgaaattcc gattcagggc cacggccgct tccaaagcga tgcccacgtt
     3181 gagcaacagc gcggatgcgg cgcggctcgt gccgctgaat ccgctgccca cggcgctcat
     3241 ccgaccgact tgcagctttg ggctgacagc aattgcggcg ccgctgctct cagcgatcga
     3301 ccagtgcgcg agtgcttcgc cgagggttgc gctgatgtcg ccgccagcgc cgtcacggag
     3361 ctcggcgcgt ttggccgcga cgaggctggc gccgagctcg aatgagagcg acccgtgagg
     3421 cggagcgaag cgtgcaccga cgaacagacc ttcagctgct tgtggcacct gacctagtgt
     3481 acccagcgct ccaaccgcga cacgcgtccg ccagggtcgc ggtgctgggg gagaaactcg
     3541 tgatgctcgg ggagaggttc gtgatacggc cggcgcggtc accccgacgg gagccgaagc
     3601 ttttggcgcc gaatcgggcg gcggcgccgg ccccactgaa ggcgcggacc tggggaccgg
     3661 cgttggcgcg ggttcggcgc caaccagatt cggctcgatc atgagcgcga gggccagtgc
     3721 aacggcgtcg gaggcttcgg cgcagttcgg ttgcggcagt agcagttgac gctgcccgag
     3781 ctgctcacca gagacggtag atgccacgat catggcgctc caaccgcctc gttcgccaac
     3841 ggtcagcacc acgtcgaaga gcacctccgc gtccgaggga ctgacgaacg caccgcgacc
     3901 caaatgcgcg tcgacctggc gcgatagtgc ccgcaaatcc ggacagctct ctgcaccggc
     3961 gaggcgcacg aggttcaagg ccgaaggcgc gcccctgcct tgcgcgctga cgtgcccggt
     4021 gaccaggagc gtgaccccga gcgcgaggcc ccaagtgcta gagtttcggc gagcgtgggt
     4081 gagcgagctt cgacgggtca cagagacggg acgctggggg attcgcgaac catagcggaa
     4141 aaggccgatc ttcaccggag ggctgagtgg gagcacatct gaatgaggaa agccagcccg
     4201 aagtgactag tgggttatgg tctttggccg agccggttgc gaccccgacg ccgccgcttc
     4261 gctcacttcg aaagcgatgt cgcgcgtcac tcacaacaac tggtgtgtct acgccaagcg
     4321 gccctttcgg tgcactgagc atccactggg tagccttgcc gcacccgcac gttaagtgca
     4381 gatcgttggc cagaccttgc cggatgtcac tcgaccgcgt cccacatcga gttatcgctg
     4441 acctcgtcga gtcgtgccaa tcagtctccg atgccgaggt tctcgccgcg ctagaggcgc
     4501 ttccaccgct tctcgacgag gcggctccgc agtggcaacg tgatgaatac tggctcgctg
     4561 ccgcctaagc cctgcgcagc ggctcatccg aatggttgtc cagccgtaag tctttgcgga
     4621 aggagcgttt gcactccatg acgatttgtt gtcggcgtcc gttgctcttc tcgcaggcct
     4681 tgaccgcatc gggagcatgc ttccacgcgg tcttgccgtt gatcgccgcg cttgcttgcc
     4741 agcctgccgt gaaccagcca gctcagcaac ccgctcgcac cgcgattgcc attggggaga
     4801 cgtgcacgcc ggacggcaag agtcctccag agatcctcga cgagaacctg caacctatcg
     4861 acttgaccaa cgagttgtcg atcgaagccg cagtggggca tcgggtggtt ctctgcgctc
     4921 cggtgcgccg tgagctacgg ggtcgcgttg ttgatgaaaa cgatcagccg gttgcgggcg
     4981 caacggtgat tgtcgagtcc tggcaaacac cacctccgat tggcggactg gaacagcgtc
     5041 gacggctgct gcgtacaaca aagacagaaa caatggcgag cggtgcgtgg cacgtgcccg
     5101 cagagtctgc ttggatgctg ggaattctcg cggccgacgg ctttcctttc tttgtgagca
     5161 gctactgcgt tctagcggtt ggcttcgccc cgttcgtttt cgacccatgg caacatcccc
     5221 acgccaacgc gggttcttca gtgggtgaca ttgtccttcg cgagtccgaa ccgcgtaata
     5281 taccggcgct cggtgctgaa acctcctcat gcggcatagc gctggagcca tcactgtaga
     5341 cgatatggcg gcccactcag ttgggtacca ggaccgcggt acttgcccac tgacaagttg
     5401 gggagccgat atggattcat cagtgtctca gacttccaat ctttgttcag ccggagccga
     5461 gcccgcgctg cgacagcgta ggattcgagg aattcgcacg cgacgctgcg gagttgccga
     5521 attcctcagt caggacttgc cagcagattg ctgaaggggc attcggcggc agggggtggt
     5581 catcagcgcg agcaacgctc tgcctagcag acgtccaaga actcgggcaa cgcgattaag
     5641 tttcgtttgc gcagagattc tgggccctga gttcttgccc gcgcaagcgt ccttggcatc
     5701 cgctcgattc caggatccag caagagcggc tcgccatggg cgccgacaga ggcggtccaa
     5761 ggcggaggtt gcgcgccgac tcgttgtgcg agcagttcag cgagcccagc ggccactaca
     5821 agctcgcgct cattcatgtc agtggggcgc tcgcacagtt cccaccgcca acgcgagcga
     5881 ttggcgtccg caacccactg tcgcgcggcg agaagatcac cttgaagaat ggcgcggaca
     5941 agatcacgta gttccatgta tagactccca cagatcatcc agcgcgtacg ccgccttgtc
     6001 tcgctgatgc ggcaaaacga gcggttcgat cagcgcccgc acatcctcta acgagccctg
     6061 cacgttggag agaagcaagc gagcatcgct ccggtcgatc tcgtcgcgcc aggcggccag
     6121 cttcattgca agtagctgtg caatcgaggg ggcgcgcacg agaagcgact cggactcata
     6181 gaggatcggg ccctccgtta aggtcacgat gtaggccttc gcaccatcgt tcagccagtc
     6241 ctctggcaaa tcaagtgaac cggcgacgcg ggccgcggcg ctgcgaacta ccgcggctga
     6301 tggcttcaaa aagcatgcgt cgacgtcctt agtcgcctgt cgcgccccat agaggaggac
     6361 caacgcggcg ccaccgacca caaggagctc cgctcgctct gttgcgctct cgagttcttc
     6421 agagagtgca tccaacgcgg tcctgatgtc acctgcagag agtggcacag ccgcaacttt
     6481 agcactgtcc aaataggagg gcccttggca ccgcgacgtt gacgagagtg cacgaactgg
     6541 gtctcggtcc acgggcctgt gccgcctttc gagcacgaat gctccgataa tcaattggtc
     6601 atctcgagac tcggcttctg ctgaccatcc cgcccgggcg attgtcaggc ccagccgttg
     6661 cagggcgcca acatggtgga gccgtcatcg gttgctgcta tcagaacgca gcccgcgttt
     6721 cgttgaacct gctcgcggcg gccggcaggt gcttcggttg cgcatctcgc tagcaactcg
     6781 tagggcttgt atcgggctgc catcgccagc gccaatcacg gcgttgcttc tcgtgagttg
     6841 tcatcgggat agccctgtcg cgtcagacgc aaaccgcgac atcggtgcta tgaagaggcg
     6901 catggaccag gaacagaccg aaatctggga agcagtgttt gccgacaagc agacaatgtg
     6961 gggactcgag ccgacgcggt ccgccatcct ggcgaaggac tacttccttc gaatgggcgt
     7021 ccgaaatgtg ctcatccctg gtgttggcta cggcaggaac gccaagctgt tttgcgacca
     7081 tgggatgtca gtgacaggaa tcgagatctc tgagaccgcg atttctctcg ctcgatcgcg
     7141 gctgggcctg gatattccca ttcaccacgg ctcggtggcg aacatgccat tcgatactcg
     7201 gcagtacgat ggcgtcttct gctatgggct catctacctg ctcgatacgg acgggcgtgc
     7261 aaagctcatc cgagattgct gggagcagct cgcacccggt ggctcgatga tcttcacggt
     7321 catctcgaaa gaggctccga tgtacggcca aggacagcgg ctcggacagg actggtacga
     7381 aatccatcct ggcatgaaga tgtacttcta cgatgccgac tcggtcgagc gcgagtttgg
     7441 tgaccatggt cttgtcgatc actccaagat agatgagccg ttgcacaacg gcagctcgtt
     7501 cccgttcata aacgtggtgt gcaggaagcg ttagaccttc gagattgtct gggaagcaga
     7561 tcgacattca gaaagagtga tgtacctcgt tttttctgat gcggtcgtag tatagcagcg
     7621 ctacattaag cggactcaag caggacgtga gacgctgaac acgcatagcg atcgagatcg
     7681 cgcggaatca gagctcgtgc ggcttacgcg ggccaccgct ctttcaaatc tcgcggaccg
     7741 gctcgaggag gccatacccc agcatgcgct ccacctcgcc gtcgaatctc cgaaacgcga
     7801 gcatgttcag cgcctggaac caccacggga gcggcgcagg ccggccccgg cgcttcccct
     7861 cgacgctgag gtacctcatc tcggtgagcc tccatccctg ctcctggaag aagccctccc
     7921 agtcgatcgg ggagaaccgg cgtggcgcat tcctcaggcg cttccggagg tcgcgctgcc
     7981 gccgcatctg cctgttcaag aagggcgacg agtagtccgc gatccacgca cggtagctcg
     8041 cgtgcgcgcc gagatcgcgg gcgagcgcgg ccacttcttc ctgtgagaga tacgggatca
     8101 cgccctcggt gagcaccagg atcttctctg cccgcttggc gatctggtcg agcagcccgg
     8161 tccgctcgct cgcgttggtg aggtcggccg agatccgctc gagcgcgcac gaggcgacct
     8221 cgcccgcgag ctggtggttc ttgagctcga taagcttcgg gtgatcgacc tcgaaccatt
     8281 ggaagccgct cgggagctcg agccggtagg gccgagtgtc gaggcccgcg ccgaggttga
     8341 gaaccgcgtc gaaccccttg gcgatggcct catgaatgag cgagtcgatg acggaagtgc
     8401 ggaccacgat tgtccagcca aattgccgtg cgccggccat ggtcaacgcg atcgtgcggc
     8461 cgcgctcgcc cgcgagccgc cctgccagag gatcgcggaa tagcgcgtcg ggtcgctcac
     8521 tctccatcgc acggtagccc gcaacccaga tcgccgtgtc ggctacttca tggatctggt
     8581 catccgacac gggtttcatc ggcaggaggt cctttcttgc gcgctagcct actccggtgg
     8641 tcagcgtatc aggagcgcgg aagaacctcg actgccttcg tcgcatcgaa gcaccactcc
     8701 aggactcggg cgacgcaacc aggggaagca ttgagctccc gcgtaccgat ggtcgagcat
     8761 ctaagccaca cgtctttgcg accgttgagg cctgggaact ccggacccga cacgtagacc
     8821 ggaatggagt ccgttggcca cgcattccac ggttcgaagg ggggaaagac aaccggtagt
     8881 gataccgaga actgcttgtc gattgacgca attctgagcg tccactcatc gttggcgtcc
     8941 cagacgaacc tgcgccctcg gaatgtgagc ggacgcctcc gtttggtggc gatcgccatg
     9001 gctcgaggat gcaccgatcc tatcggacca gtaccgtcgg tggaaagtcg aagccccacg
     9061 gattgtcggt gttggtgata gagctcggaa aggcggtgac atgccgactc gagatcggga
     9121 cggttgggcg attcaccgag atggggggca ccgctcgcgg aaagcgcgaa gccaacgtca
     9181 gcagttgcat gtactgaatc attgcgagct gttgctgcaa tactgccatc tgttgggcgg
     9241 cgagcgcagc accctggtaa gtatcaccga catgaatgtt ctgttggaca ttgcccatgt
     9301 agacgacact tcctcggtcc gagtttcgag aggcgagtac gacctcagcc tccggtttcg
     9361 aatccgctgt cgggtctagc tgggtctgct gctggttgct cggggagggt gcggagagcg
     9421 caaccgaggc ttcgcgggtt gtagggctct cagcgggcgg tggggtgttg gccagcgcta
     9481 cgtctcgggg agcggcggcg ccctgcggtt ggagagtggc tgtcttgtcg gcaccagcgg
     9541 gcgcgctcga gcctcccgcg cttgatgggc gatcgttcgg agatgcggcg tcactcgatg
     9601 cgacgagcgc tgtcccacga cggtagtcgg actcgaggcg agccaggacc gccgctacgg
     9661 agcttccgac ttcgttcgtc ggctcggtgt ccgcggcttc ggtccctccc gcaacggagg
     9721 gaagcaatac tccagtgccg ctcggaatgg cgctgttcgt gggcacgctg accacggacg
     9781 gcgttaaagc agacttggcc tgcgttgctg cgccctcggg tgcgtgctcg tctgccgaga
     9841 cgctgttcag acgacctagg caatcgagga tcatggcgcg cgcgtccacg actggcgtgc
     9901 tcgactggtt ccgatctgcc tcgctctgca acagtacgag aatcgccgcg ccgctcaggc
     9961 cgccgccagc gaaagcccag attaggggga cgcgcttgtg cagcatgcga aggagcctag
    10021 cacggtacgc gcggacccgg gttcagtggg cggctgggct ggccgaacgt cctttgtgac
    10081 cctggctcgg cgctcgagct ccgaggcggc tgagctggcg cgtcgccaag ccggcgtcag
    10141 gggcgtggcc tgttggtttg tacgtttctt gcttcgcttc atgtgatcgg cagcgcgtag
    10201 tgcatcactg acgtagcagc cgtcggatgg taggcggcac cgtagatctc gaggtcggga
    10261 ccgcccgtgt gtcggtagcc cttctcagac tgcgcaagcc acgtcgaata ggcatagctg
    10321 accgtatgat cgatctgttg cgccgggccg cggtgctcga acctcgcgta gctcgctgct
    10381 ggaacttgga acgagaccat gcctttcgga ggatgggcgg cttccgtgac ctcgaccgcg
    10441 gcgtggtact ccaggcggtc gctatcgtgc tgttcctgac gaacgacacc gtagcaaacg
    10501 cctggtactc gcccgtcgat ttcgggcagg cgatcgagga atgccttcca caatggtggt
    10561 agccgagcgc cgatgttgtt cttctccgag tctgtcccga agaactgcgt gcgcatgccc
    10621 acgaggttca tttgtttctg gaaacgaacc tcgggctcca gcgaaacatt gtggtgaatg
    10681 tgggtcagat aatcggcatc gaattgtggc tttttcacga acacgttccg tttgcccaac
    10741 tttcgatagt cctgtgccgt catgccgaac gccttcttga aggcgcgcgc aaatgcctcc
    10801 tgcgattcaa agccggaaag gagcgcgatg tccaggaccc gcagctgagt cgaggacaac
    10861 aactcgagcg ccttcgcgaa gcgacgcgac cggatatagg tctttagggt ctcgccggtg
    10921 agcgacttga agatgcgttg aaaatgccat tggctcagcc ctgcttcctt cgcgatccga
    10981 gcgatctcga gatcatcatg aagccgcgcc tcgatgtagt cgacgccgtg ttggatccgt
    11041 tccaggtagt agctcaagcg tcagttctca ggggtttcga agaagccggt catgctggtc
    11101 aggcgcccct cgtcatcgta ttcgccatag ctgatgccat caccgatcac gctgccctcg
    11161 ccgttgcgca tagtccagcg tgccatgctt ttaccgtggt gggagatgaa ttgctccgtg
    11221 acgaagtggc caccagggac ctgctggtgg aaggccacca tgtattgcag gagctcgtcc
    11281 cagctctcag tgcgtgtcaa cgggtcggtg tagacgcagc gtttcacgag gcagcggccg
    11341 aagagctgcc gtttactctc gctcgagctg actttccaag cttcgacgta tgtttcccag
    11401 gttgctctgt agctcgcgct catcagattc tccatcgttc tcgcgaccac catacggctc
    11461 gggtgcgggg ccatctgacc gtttgtgcga ttcgccgaga gtcagcgcca atctcgggcg
    11521 cccgtgccac gtcgtccgag cgttctttgg tgcggcgtgc gcggtccaat caagaaatgt
    11581 gactgacggc ggtgagctgt cgggggaggc gacgataggt cgcgctcgtt acggcgaggc
    11641 cgcctgaaag aaccaagaga cgtcagctcc agcgaagtcg gggtatcgga tgaaatgccg
    11701 acgaaccctc gttccgctcg ggctttcgcg ggtgaacagt gctacgttcg gccgaacaag
    11761 catgccaaac acggcaaggg ataccgacgt tgagctcctt cggcgcgtgc tgccgctatg
    11821 gcacgcaacg agggcatggg ccgaatcgct tctctgttcg gcgctcgagc ttgcccaagc
    11881 gtcggacgta ctgaggtccg agcaccgcgg gcggcatcat ttcccaggca tgacctggta
    11941 ctaccgcact cacggtgccg gtgttgacat cgatcgcggc gtggtctgtg gcggaatcga
    12001 tttcgacttc gacaaaatag agcccgatcc gtggcgactg cggatcttcg tcgagaagca
    12061 ggtgaatgcg ggtacgctca acgccgagta cgccgagctc gttcaggacg aggatcgttt
    12121 tgacgcagcg gctcgagagc tcttgggatg agggcgtgca atgtctgaat aacgctgcgt
    12181 acgcagaaca ggtattcagg accgtcagcc tcgccagtcg aaattgaccg gcaacgcacc
    12241 gtcctcaggc tcgaatcgct agcaagcaaa acccgttgcc gaagggatcg gcacagaacg
    12301 ccgccgctcc ccagtcacct cgctggagcc cctcgaccgt cccgcccagg cgagtcacct
    12361 cggctacggc cgcatctacg tcgtctacgg cgaagtcgag atggactggc gtccaatggc
    12421 gttcgtaggt ccgagtgcag gacgccgttg cggttgcctc cgttccccct tccttgagca
    12481 aaaggtgcaa tttcgtgtcc cccaccagaa gcgtgttgtg ggacgcttgt tccttatcga
    12541 gcgaacaccc gagcgcctga cagtagaatg tcgtcgcagc tttcaagtcg ggaacatcga
    12601 cgcagattga gactctcggc atgcggtcag cgtatcagct ttcgcccgtt gcttcccgaa
    12661 tcgtagaaga cgtcaacatc cgaccgagcg ggccatcgcc atgtgcaagc gccgcccgag
    12721 gtaagtggcg cctgtatcag aatgccggta cgttggtcgg gtccgagacg tggtttcatt
    12781 gggcgcccac gtgggccgcc agcccggcc
//
LOCUS       DASTKU010000590         2696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1453936, whole genome shotgun sequence.
ACCESSION   DASTKU010000590 DASTKU010000000
VERSION     DASTKU010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453936"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFQ61_20705"
     CDS             <1..346
                     /locus_tag="VFQ61_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HET9956935.1"
                     /translation="VATIEPTLTCLDFLAIFDSEKAAPKLHTEVPKLAHRWLGGLERR
                     LEEREWIACADFTVADIMMAGVLRVIRKTDLLEPFPRTKAYYDRCFARPAWQRTLNLY
                     AERLGVSADSIR"
     gene            complement(516..920)
                     /locus_tag="VFQ61_20710"
     CDS             complement(516..920)
                     /locus_tag="VFQ61_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956936.1"
                     /translation="MIDVGILLKSDEMIDFLESFDLPVQYHFDRLHEGESDSYSVESD
                     DISLELHFDADQRCTTIFIRDPEAALSHGLVSFPHLHSPAAVESYARNHGLTLRRGPN
                     WLRCDGPTRCVHYQFAADKLTMVTLMSPDTAP"
     gene            complement(1148..1891)
                     /locus_tag="VFQ61_20715"
     CDS             complement(1148..1891)
                     /locus_tag="VFQ61_20715"
                     /inference="COORDINATES: protein motif:HMM:NF025602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET9956937.1"
                     /translation="MPTLILSPRQTDDGQCLWRVAIARGWDVQRLRTWRIDEAVLGAD
                     DPFLYVEALFGATLAEQLGVTLLNPREDWLSSLPERYSRRKVVFSTLAEARKASAPAF
                     VKPPNDKSFPAAVYSPGELPAAFDGEMSVLISEPVTFVSEFRCFVLERKVQTFSLYAR
                     HGLPTSEATSPDAESAHMLSFVSQVLQDHEVDIPRACVLDCGLIDGRGWAVIEPNAAW
                     GAGIYGCDPDKAIDVVRAATVRAAPIPRS"
     gene            complement(1979..2314)
                     /locus_tag="VFQ61_20720"
     CDS             complement(1979..2314)
                     /locus_tag="VFQ61_20720"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9956938.1"
                     /translation="MWGSRVFIEPHARALVGLGGFKGPPSSDAVVEIGYAIAPAFQGQ
                     GLATDAVAQMVRRAFEHASVRAVDAHTLGHANPSTRVLEKSGFRKIGEVRDPDEGLIW
                     HWRHERAVP"
     gene            complement(2336..2491)
                     /locus_tag="VFQ61_20725"
     CDS             complement(2336..2491)
                     /locus_tag="VFQ61_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956939.1"
                     /translation="MIPSRRKLVDGNLELMTAALAGGAALEALLGVSVADGWAGFPEA
                     LPVLCES"
BASE COUNT          531 a          886 c          796 g          483 t
ORIGIN      
        1 agtggccacg atcgaaccca ccctgacatg cctcgatttc ctcgcgatct tcgatagcga
       61 aaaggctgct cccaagctcc acaccgaggt gcctaagcta gctcatcgct ggctcggcgg
      121 tctcgaacgc cgcctggagg agcgcgagtg gatcgcttgc gccgatttca cggtggccga
      181 catcatgatg gccggggttc tacgcgtgat ccgtaagaca gacctgctcg agcccttccc
      241 gagaaccaag gcttattacg atcgctgttt cgcccgccca gcctggcagc gcactctgaa
      301 cctctacgcc gagcggctcg gcgtgagcgc cgatagcatc cggtaggctg cgtgcactcg
      361 aaagggactt gttacttcgc aatcggcatc acgctctcaa gtgcttggtt caacggagtg
      421 ccacgcctgg caacgcattc taagatcgtg aaggccgagc acttgtccaa tcgggaacga
      481 actcgccagg acttgcgccc atcgcggctt tggtctcaag gagcggtgtc gggagacatg
      541 agggtgacca tcgtgagttt gtcggccgcg aattggtagt gcacgcaacg ggtggggccg
      601 tcgcaccgga gccaattcgg gcctctccgg agtgttagac cgtggttcct ggcgtacgat
      661 tcgaccgcgg cgggagagtg aaggtggggg aacgacacca gcccgtggga gagtgccgcc
      721 tccgggtcgc gaatgaagat ggtcgtgcag cgctgatcgg catcgaagtg cagctcgagc
      781 gagatgtcgt cgctttcgac actgtacgag tcgctctccc cttcgtgcag cctgtcgaag
      841 tggtactgca cgggcaggtc gaacgactcg aggaagtcga tcatttcgtc gctcttgagc
      901 agaatgccta cgtcgatcat ctgatgccga gtgcaaccat agcgcaacgc ccgcccagcg
      961 cttgaacgtt atcctggcga accagatgca gggacgcggt cgtccgacac gacagctcca
     1021 ctgccaacga tttgaacgaa cttcgccagg ccggcacgtg tctcgaatcg tagcaaaaca
     1081 cgccgcgagc ccgcaaccaa gccctcttca gcacagaaac cgcctctgat cgatcgcagc
     1141 cctggaccta ggagcgcggg atcggtgctg cgcggaccgt cgccgcacgg accacgtcaa
     1201 ttgccttatc ggggtcacat ccgtagattc ctgcacccca ggcggcattc ggttcgatga
     1261 ctgcccaccc gcgtccatcg attagaccgc aatccaggac gcacgcccgc gggatatcga
     1321 cctcatggtc ctggagtact tgcgacacga aagacagcat gtgtgccgac tccgcatccg
     1381 gcgatgtagc ctccgatgta ggcagcccgt gccgcgcgta caacgagaag gtctgaactt
     1441 tccgctcgag tacgaagcat cgaaactcac tcacgaacgt gaccggctcc gagatgagga
     1501 cgctcatttc tccgtcgaac gccgcaggca actcgcccgg agaatagaca gcggctggga
     1561 agctcttgtc gttcggtggc ttcacgaagg ctggcgctga agctttccgc gcctcagcga
     1621 gcgtggagaa caccaccttg cggcgcgaat accgctcggg aagggagctc aaccaatcct
     1681 ctcgtgggtt caacaaggta acaccaagct gctcggcgag cgttgcgccg aatagggcct
     1741 cgacatacag aaacggatcg tccgcgccca gtaccgcctc gtcgatcctc caagtccgga
     1801 gccgctgcac atcccatccg cgagcgattg ccactcgcca cagacattgc ccgtcgtcgg
     1861 tctggcgagg gctcaagatc agcgtcggca tgggtttctg tgcggctaat gtaccgcgat
     1921 tgagcaagcg gacgcatgtc gaacgtatcc gtttccgccc caccgaccag ctcgcgagct
     1981 acgggaccgc gcgctcatgg cgccagtgcc aaatcagccc ctcgtcgggg tcccgtactt
     2041 cgccaatctt ccgaaatcct gacttttcta gcacgcgcgt cgacggatta gcgtgtccga
     2101 gtgtgtgcgc gtcgaccgcg cggaccgatg cgtgctcgaa cgcacgtcga accatctgcg
     2161 cgacggcgtc cgtggccagc ccctgcccct gaaacgcggg cgcgatcgcg tatccgatct
     2221 cgaccaccgc gtctgaagag ggcggcccct tgaagccccc cagccccacc agcgcccgcg
     2281 catgaggctc gataaacacc cgcgagcccc acacgtgccc ttggggattc tccctctacg
     2341 actcgcacag aaccggaagc gcctccggga aacccgccca accgtccgcc acggacacgc
     2401 cgagcagcgc ctcgagcgca gctcctccgg cgagcgccgc cgtcatcagc tcgaggtttc
     2461 cgtcgacaag cttccggcga gaggggatca ttggactcgt cgagctatcg atggggtcag
     2521 tctccgcgtc aacctgtagc gcctctgttt ccgcaacgaa gatccgacgg cccgctgcga
     2581 cggctcggtt gccccgcacc gcaaacaagc ctagcgtagg ccaatggcgg gaccgagcgc
     2641 agccgttttg tttcgctcac gatcagagca catgtccgaa gaaggtggat atttcc
//
LOCUS       DASTKU010000591         4804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1454342, whole genome shotgun sequence.
ACCESSION   DASTKU010000591 DASTKU010000000
VERSION     DASTKU010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454342"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFQ61_20730"
     CDS             complement(<1..839)
                     /locus_tag="VFQ61_20730"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET9956940.1"
                     /translation="MTLTPAEPTPFRIPRGLRRVHERFVAGNRIHLLHDGSQAFPAML
                     AAIDSATHQVLLEMYWFDSDRIGRRFAQALMRAAQRGVEVAVIYDAVGSFGADPAQFD
                     EMQRAGVHVVEFNPVAPWKRRFRIARLTRRDHRKILVVDGRVGFTGGINIADEWLPEE
                     EEGGGFRDDMVCVEGPAVWGFVESFQRTYRRCGGLPLTRTILQSAVREIGAGEIETRG
                     QAAVTAEATQHVRVLGERFRRRDGSISRAYLSRMYRAHERIWLANSYFIPDRNVVRAL
                     IRAA"
     gene            975..2066
                     /locus_tag="VFQ61_20735"
     CDS             975..2066
                     /locus_tag="VFQ61_20735"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HET9956941.1"
                     /translation="MSRLRLRRWFSGSLIGAALVVMGSCSRERPRPAVYPTPAYGTAG
                     PYGTNTQGYPQQGYPQQQPYPQQGYPQQQPYPQQSVPQPNPPQVAVPLPASPLDPINR
                     VDIGFLRGRALAVLGELVANLPEPQQSRVRGIPLVTDDTVGEVNAFATCTRSGQTAMA
                     ITDGLLDIQAHLAQARAIDELFGSRKTDEYIQFIARNQRPKSPIVQPPLGMFDANQSA
                     DGRKVARQHDVLDEQLAFVLGHELAHHYLGHLPCTGASGLSGAEVARVLADAVPMFNQ
                     GFEIAADSAGINNVLTTGGRRQGYHFTEGGALLTMQFFAGIDQFSAADILFGFERTHP
                     PPAVRTPIIQQAAALFRATGGRGLPVIGF"
     gene            complement(2088..2888)
                     /locus_tag="VFQ61_20740"
     CDS             complement(2088..2888)
                     /locus_tag="VFQ61_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HET9956942.1"
                     /translation="MTRAFPRGRPPTLVNASGTWQIPARMGIFDRMGRVISSNFNGLL
                     DRLEDPGKSVESLLENMRDQLRAARQEVVRAVAAEKQLREKVKTLDAEAEKWNQRAEL
                     ALKRGDESLAREALLQKQRVVGERDRAEALASEQLAAAHEMKNELPRMEQKIQEFSLR
                     KSTIATQAQMAKHGSGVESLGRTGGASPGPFEEMRRIEDQIEGVEASIQAQRELDQAL
                     GTRGPSGLSRAELEAKFRNLEANEPGPSGTPEIEAELKALKQKIRIKE"
     gene            complement(3037..4446)
                     /gene="lpdA"
                     /locus_tag="VFQ61_20745"
     CDS             complement(3037..4446)
                     /gene="lpdA"
                     /locus_tag="VFQ61_20745"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET9956943.1"
                     /translation="MESSFEAIVLGGGPGGYVAAIRLAQLGVKTVCVEEEEMGGVCLN
                     WGCIPSKALIANAHFYEKSKHIADHGIFVNQVTVDENKMQDWKNGIVKKLTSGVRGLL
                     KSNGAQIIDGRGKLVGPRSVEITLKDGSTRRIEATRAIIIATGSATIQVPGFEFDGER
                     VIGAREAVSLRKTPKRLVVIGGGVIGLELGMVYQSFGAELTVVELTPSLLPGIDPDCV
                     RVVERNLTKRGAKILKNTRADGYERGPNGEVIVKVTTPEGPQRLECDNVLVAVGMKPR
                     SRGIGLEEVGVEIDPRGFITTDERCETNVKGIYAIGDVSGRPMLAHKASKEGEVCAEV
                     IAGHPAAKDWVTVPGIVFTDPEIATVGLTEQEAKDQGLTVKSGRFPFSVLGRAMSIGE
                     TDGFVKVVTDTKSGRVVGIHIVGPSASDLISEAVLALETVATAEDIAMSIHPHPTLGE
                     AMMEAAAASLGHAIHIANR"
     gene            complement(4503..>4804)
                     /locus_tag="VFQ61_20750"
     CDS             complement(4503..>4804)
                     /locus_tag="VFQ61_20750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956944.1"
                     /translation="SSSDDPSRYRDEGEVELQRELDPLLRLDRYLRRETLETVEDASL
                     DAEWERMLEASLSRVEALPPPALQTLFDDVFAALPWHLREQRALLENCLERSAGS"
BASE COUNT          845 a         1622 c         1436 g          901 t
ORIGIN      
        1 gccgctcgaa tcaaggcccg caccacattt cgatctggaa taaagtagga attagcgagc
       61 cagatccgct cgtgagcgcg gtacatgcgc gagagatacg cgcgagagat gctgccatca
      121 cgtcggcgaa agcgctcacc gagtacccgc acgtgctgag tcgcctcggc agttaccgca
      181 gcttggccgc gcgtttcgat ttcgcccgcc ccaatctcac ggaccgccga ctgcaaaatg
      241 gtgcgggtca gcgggagccc gccacagcgc cggtaggtcc gctgaaagct ctcgacgaaa
      301 ccccaaaccg cgggaccttc cacgcaaacc atgtcgtctc gaaagccgcc gccctcttct
      361 tcttcgggca accactcgtc cgcgatgttg atcccgccgg tgaaccccac gcgaccatcc
      421 acgacgagaa ttttgcggtg atcgcggcgc gtcagtcgcg cgatgcgaaa acgacgtttc
      481 cacggcgcaa cgggattgaa ctccacgacg tgcacccccg ccctctgcat ctcgtcgaac
      541 tgcgcgggat cggcaccgaa ggagcccacc gcgtcgtaaa tcaccgccac ctcgacgccc
      601 cgctgcgcgg cacgcatcag ggcttgcgca aaacgccggc caatgcggtc cgaatcgaac
      661 cagtacattt ccagcagcac ctggtgcgtc gccgaatcga tggccgccaa catcgcgggg
      721 aatgcctgac tcccatcgtg cagtaggtga atgcgatttc cggcaacgaa gcgctcgtgg
      781 acgcgccgca accctctcgg aatgcggaac ggcgtgggct cagccggggt gagcgtcatc
      841 ttctccttta tcggttagcg cggcgggaat tgccagcggc tgtcctgctt gagcgaacat
      901 ccggccagag ttcgtgaccg tgacccactg catgcaccag acgaggcacc aagctcaagc
      961 tatgcttcgc gccgatgtca cgactccgcc ttcggcgctg gttttcgggt tctctgattg
     1021 gtgcggcgct ggtcgtgatg ggaagttgtt cgcgggagcg cccccgtccc gcggtgtacc
     1081 cgaccccagc ctatggcacc gctgggccat atggcaccaa cacgcagggg tacccgcagc
     1141 agggttatcc gcagcagcaa ccgtacccgc agcagggcta tccgcagcag caaccgtacc
     1201 cgcagcagag tgttccccag cccaacccac ctcaagtagc ggtgcctctg ccagcttctc
     1261 cgctggatcc aatcaaccgc gtagacatcg gttttctgcg cggacgcgca ctcgcagtat
     1321 tgggagagtt ggtcgcaaat ctgccagaac cacaacaaag ccgtgttcgc ggtatcccac
     1381 tcgtcaccga tgatacggtc ggcgaagtga acgctttcgc cacctgcacg cgcagtggac
     1441 aaaccgcaat ggcgatcacg gacggcttac tggacattca ggcgcatctc gcccaggcgc
     1501 gcgccatcga cgagctgttc ggtagccgca agacggacga gtacattcag ttcattgccc
     1561 ggaatcaacg gcccaagtcc ccgatcgtgc agccaccgct cggcatgttc gacgcgaacc
     1621 agtcggcgga tggccgcaag gttgcgcggc agcacgacgt cttggacgag cagctcgcgt
     1681 tcgtgcttgg gcacgagctc gcccaccatt acttggggca tttgccgtgc acaggagcat
     1741 ctggcctgag cggcgccgag gtggcgcgcg ttctggcgga cgcggtgccg atgttcaatc
     1801 aaggcttcga gattgcggcg gactcggctg ggatcaacaa cgtgctcacc accggagggc
     1861 gccggcaagg gtatcacttc accgaaggag gcgcgctctt gacgatgcag ttcttcgcgg
     1921 gcatcgacca gttcagcgcc gccgacatcc tgttcggctt cgagcgcact cacccgcccc
     1981 cggcggtacg cacgccaatc attcagcaag cggccgcgct atttcgcgcg acgggtgggc
     2041 gcggcttgcc cgtgattggg ttctgagcct ccgcgcgggc ctcgagtcta ttctttgatc
     2101 ctgatctttt gtttgagagc cttgagctcg gcctcgatct ccggcgtgcc gctcggcccc
     2161 ggttcgttcg cttctaggtt gcggaacttg gcttcgagct cggcgcgaga aagcccgctc
     2221 ggaccacgcg tgcctagggc ttgatcgagc tcacgctgtg cctgaatcga ggcttcgacg
     2281 ccctcgattt ggtcctcgat ccgccgcatt tcctcgaatg gtccagggct cgcgccgccg
     2341 gttcgtccga gtgactccac gccggatccg tgcttggcca tctgcgcttg ggtcgcgatc
     2401 gtcgacttgc gcagcgagaa ctcctggatc ttctgttcca tgcgcggcag ctcgttcttc
     2461 atctcgtgcg cggcggcgag ctgctccgag gccaaggctt cagcgcggtc acgttcgccg
     2521 accacccgct gtttctgcag caacgcctcg cgggccaagc tctcgtctcc gcgcttgagt
     2581 gccagctccg cgcgctggtt ccacttctcc gcttcggcat cgagcgtctt taccttttcg
     2641 cgcagctgct tttcggcggc caccgcgcgc acgacctctt ggcgagccgc gcgcaactga
     2701 tcgcgcatgt tttcgagcaa tgactcgacg gacttaccag ggtcttccaa gcggtccagg
     2761 agaccgttga agttgctcga gatgacgcgg cccatgcgat cgaagatccc catgcgggca
     2821 ggaatttgcc acgttcccga ggcattcacc agcgtcgggg ggcggccgcg cgggaacgcg
     2881 cgggtcacaa aacgagtaga gcgagaatcg ccgtgcgatc tcgccctact gcgctgcgtt
     2941 gcggcagccg gcagtcttcc ggcctgtgtg ccctgagaag cggcgccatt cgagccagga
     3001 cgccgacgcc ggcaccccag cccaaggcgc tcgagctcag cggttcgcga tgtggatcgc
     3061 gtgcccgagc gaggccgcag cggcctccat catggcttca cccagcgtgg ggtgcggatg
     3121 aatcgacatc gcgatgtctt ccgcagtggc cacggtctcg agcgcgagca cggcctcact
     3181 gatcaggtcg ctcgcggacg gcccgacgat gtggatcccc accacgcggc ccgacttggt
     3241 gtcggtcacg accttcacga agccgtctgt ttcgccgatg ctcatcgcgc gaccgagcac
     3301 cgagaacggg aagcgacccg acttcacggt caggccctga tccttggctt cctgctcggt
     3361 gaggcccacg gtcgcgatct cgggatcggt gaacacgata cccggcaccg tcacccaatc
     3421 cttggccgcc ggatgcccgg cgatcacttc ggcacagacc tcgccttcct tggaagcctt
     3481 gtgcgcgagc atgggtcgcc cgctcacgtc gccaatggcg tagatgcctt tgacgttcgt
     3541 ctcacagcgc tcgtccgtcg tgatgaagcc acgcggatcg atctcgacac cgacttcttc
     3601 caggccgatg ccccgcgagc gcggcttcat accgacggcg accaatacgt tgtcgcactc
     3661 gagccgttgg gggccttccg gagtcgtgac cttgacgata acctcaccgt tggggccgcg
     3721 ctcgtagcca tcggcgcggg tgttcttcaa gatcttggcg ccacgcttgg tcaggttgcg
     3781 ctccacgaca cgcacgcagt ccggatcgat tccgggcaac agcgacgggg tcagctccac
     3841 gaccgtcagc tccgcgccaa aggactgata caccatgccg agctcgagcc cgatcacgcc
     3901 gccgccaatc acgaccaagc gtttcggagt cttacgcaag ctgacggcct cccgggcccc
     3961 gatcacgcgc tccccgtcga actcgaaacc gggcacctga atcgtcgccg agccggtcgc
     4021 gatgatgatg gcgcgggtcg cctcgatacg ccgcgtggag ccatccttca gggtgatctc
     4081 gacgctgcgc gggcccacga gcttgccgcg cccgtcgatg atttgggcgc cattcgattt
     4141 gagcagcccc cgcacgccgg aagtcagctt cttgacgatg ccattcttcc agtcttgcat
     4201 cttgttctcg tcgaccgtca cctggttgac gaagatgccg tgatccgcga tgtgcttgct
     4261 cttctcgtag aagtgggcgt tggcgatcag ggctttggag ggaatgcagc cccagttcag
     4321 gcacacgccg cccatctcct cctcttcgac gcacacggtt ttcacgccga gttgagcgag
     4381 acgaatggcc gcaacgtatc caccgggacc gccacccaat acgatggctt cgaaagagct
     4441 ttccatggcg gacttcgcat tagcgaagtc cctagcgctc tacaagcacg gctcaggctg
     4501 gctcacgagc cggccgaccg ctccaagcag ttttcgagca gcgcgcgttg ctcacgcagg
     4561 tgccaaggca gagccgcaaa cacgtcgtcg aacaaggttt ggagtgctgg cggcggcaga
     4621 gcttcgacac gagagagaga ggcctccagc atgcgctccc attccgcgtc gaggctcgcg
     4681 tcttcaaccg tttccaatgt ttctcgcctc aaatagcgat cgagccgcag taacggatcg
     4741 agctcccgct gcaattcgac ttctccctcg tcacggtagc gacttggatc atcgctcgac
     4801 gagt
//
LOCUS       DASTKU010000592         6573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_115046, whole genome shotgun sequence.
ACCESSION   DASTKU010000592 DASTKU010000000
VERSION     DASTKU010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115046"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VFQ61_20755"
     CDS             <1..926
                     /locus_tag="VFQ61_20755"
                     /inference="COORDINATES: protein motif:HMM:NF014113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956945.1"
                     /translation="TSACTDTPPPNGDTCANAVKFGWCGVDWMKGACARSCGSCSGGP
                     PSGNGGSGNSSGGSSSSSGGSSSSGGSSSTVPNPPRITGGTKGHATRYWDCCKGGCAW
                     KGKTAQTAKSCDANNNPLSDPDAGSSCNNGPAYMCWSLAPWAVSETLAYGYAAVKSDS
                     DCGKCYQLQFDGGSQNGSTDGTKKLSNKTMIVQVISNGRLDEPNKFDLLIPGGGVGDF
                     NACSKQWGTTDLGVQFGGFMLKCQQEKKDYEAAKSCVADMCKKTFANKPDLAAGCDWF
                     LNWFAAADNPTFEYKEVTCPDALIQRSGQRR"
     gene            complement(1268..2698)
                     /locus_tag="VFQ61_20760"
     CDS             complement(1268..2698)
                     /locus_tag="VFQ61_20760"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HET9956946.1"
                     /translation="MIGFLRRLVPKLAVGVLFAGMSTLLAHNVVTRACKIEPRAVDVP
                     AQSKLELSPGVRRFGASFVVQHPGMLEVHLRGSPEQIGFSHARLLQPEMRENEGILLG
                     HFQDSVPSFLLRRLILDVAQVRYRHVDLGMGEDRQREIAASALGFEPDPFRTMFPTYQ
                     RFVYLNALYDIALSFEHSPLIGCTTFVFSGAQIGHSGSLLARNFDFEVDPIFDRKKAV
                     FFVRENGKLPFASVAWPGLVGVVSGINVEGVAVVVHGGRAGEPRAEGRPVVHTLREVL
                     GRAHDTSEAIRILGEASPLVSHIIVLTDRQGHAVRVERVPGAPNFVQALGDAAAVTNH
                     LEGPHQADPKNQRVRASTSTLPRRERGDELVRNAARPVTPETLASMLRDRKGVGGKDL
                     KLGDRNAIDALIATHGVIMDTEGRKLWVSVAPHLLNRFVAFDLERRLAANYEPEPSIE
                     LPSIPPDADWGRVTASFPEDSAVSAP"
     gene            complement(2695..3411)
                     /locus_tag="VFQ61_20765"
     CDS             complement(2695..3411)
                     /locus_tag="VFQ61_20765"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9956947.1"
                     /translation="MKTAFVIPAYEAEKSVGVVVSDLISRITPELETTRPAVIVVDDG
                     SRDATAQRAEQAGAHVLRHVGNRGKGAALLTGFRYAAALSGDVVVTLDADGQHPPEQA
                     IALARHPASPEALVLGIRDLRRAGAPRANRFSNGFSNLWVSGFSRQRLNDTQCGLRRY
                     PLAAIASLGLAGSGFELESEVIIKAARAGVPIEQVPVEVIYPAAEERVSHFDSVRDPA
                     RIVARILYTAATTSFKRAPR"
     gene            3479..4099
                     /locus_tag="VFQ61_20770"
     CDS             3479..4099
                     /locus_tag="VFQ61_20770"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET9956948.1"
                     /translation="MIEDSELRRELASQARRQLRTRMRALRAAHPEAALELRSARIRA
                     YLEALPEFKSAQGVALFWPLTSEVDLRPLDVTARSLGKRVYYPFLEPGPERSLTGFAR
                     VDDISTLAPRGQRFFEPQAGSPHASRGEIDLVVVPALAVSTTGHRLGYGSGFYDATLP
                     DFIPPAQAVVVAYDFQLLAELPTLHHDVACHVVVTDARTLRVPSSA"
     gene            complement(4118..4711)
                     /locus_tag="VFQ61_20775"
     CDS             complement(4118..4711)
                     /locus_tag="VFQ61_20775"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HET9956949.1"
                     /translation="MRAEPVSLGAGAVLRYEAAFLSPEQASRLQAELSERVPWQQGEL
                     SFFGKKVLEPRLSAWFGDADYTYSGRRVAARPWLPALLEIRDRIELATGSRFNSVLLN
                     LYRDGRDSMGFHADDEPELGPNPEIASVSLGAARRFLYRPKRRFEKQLSGGELVLSSG
                     SLLWMGGTFQHVFRHAVPKTAAPLGARINLTFRWVTS"
     gene            complement(4736..5221)
                     /locus_tag="VFQ61_20780"
     CDS             complement(4736..5221)
                     /locus_tag="VFQ61_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET9956950.1"
                     /translation="MTQAVRGHKNPDQVRLRRDLDTYMAKRRLRSTEQRRLIIDTLFD
                     AGEHITIDGLLRQVRTVDSRVGYATVYRTMKLLADSGIVQEHKFGDGFTRYELVDEDH
                     HHDHLICLECGSITEFEEPLIEELQQRVATRYGFQVKQHKHELYGVCAECQKPNARKR
                     R"
     gene            complement(5283..>6573)
                     /locus_tag="VFQ61_20785"
     CDS             complement(5283..>6573)
                     /locus_tag="VFQ61_20785"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9956951.1"
                     /translation="AGARVVEGRLRAIVSWAGPDRAWLRLSTDPRRRADLHASTARVG
                     RLIVERIAPFITGTAALAAFAANLDPLEIGVLCVSIHAALTCPILAAAGSQYVARAIL
                     EGLRRGIAFRTADAFDRAGKVSMLAFCARGTLQLGEPEVADIDAFFGHRPSDVLSLVA
                     GAESGSQHAVSTAILRAARARGVRPDGTRSPTPQAGLGVTAVASNGQALVVGSRALML
                     KEHVSIAAVESRITALEAMGRSVLLVALGGRLIGLLGLQDGLRPGARASVQHALDIGV
                     EPVLLSGDARETCEALGRALDIDHIRPEIPPAERGDAIRRLADGGAIVAVVGRSQADE
                     VALSAADVSIALGAAGSTSSEWNVQLASDDVRDAAFAIRLAHEARREARLSLALILGP
                     AVAFAIVAGLGLAAPAWSPIGALLGGGVALVRLRARR"
BASE COUNT         1228 a         2109 c         2139 g         1097 t
ORIGIN      
        1 gcaccagcgc gtgcacggat acgccgcctc ccaatgggga cacctgcgcc aatgccgtca
       61 agttcggttg gtgcggcgtg gactggatga aaggtgcatg tgcacgttcc tgcggttcct
      121 gcagcggtgg gccgccctcc ggcaatggcg ggagcggcaa ctcctccggc ggaagctcga
      181 gcagctccgg aggctccagc agctctggag gctccagcag cacggtcccc aatccgcctc
      241 ggattacggg aggcaccaag ggtcacgcga cccgctactg ggactgctgc aagggcggct
      301 gcgcctggaa gggcaagacg gctcaaacgg caaagagctg tgacgccaac aacaatccgt
      361 tgagcgatcc ggacgccggg agctcctgca acaacggccc cgcatacatg tgctggagcc
      421 tggcgccctg ggcggtgagc gagacgctcg cttacggcta cgccgccgtt aagagcgatt
      481 cggactgcgg caaatgctac caactccaat tcgacggagg ctcgcagaac ggctctaccg
      541 acggcacgaa gaagctctcc aacaagacga tgatcgttca ggtcatcagc aatggaaggc
      601 tcgatgagcc gaacaagttc gatctcctga ttcctggtgg gggtgttggc gatttcaacg
      661 cctgctccaa acagtggggt accaccgatc tgggcgtcca attcggtggc tttatgctga
      721 agtgccagca ggagaagaag gattacgaag ctgccaagtc ttgtgtggca gacatgtgta
      781 agaagacgtt cgcaaacaag ccggatttgg ccgccggctg cgactggttc ttgaattggt
      841 tcgccgcagc agataaccca acgttcgagt acaaagaggt cacttgcccg gacgcgctga
      901 tccaacgctc gggtcaacgt cgctgagccg cgcggcagaa gtctgaaccc agacgcttgc
      961 gcagacgacg ccctgagctc ccgctgattt agcaacgctc agggcgttgt tgtattcgcg
     1021 ccccgagcgt ggatcttggg tttagcgaag gtgccactac acctcttgaa tgctcgactg
     1081 gacccccaat gactcacgcg gaggtaccgg attcgagcga actcaattga gtgcgaatct
     1141 attcagctca atgccgcagg cgcggtactg gaggcgcacg gtgctaacgc gtctgcgctg
     1201 gagggcggaa cgagccgctt cgcgcagcgt tgctgcgggg cggccactcc aaacaacggc
     1261 tacgccgtca gggtgcggac acggccgaat cttctgggaa ggacgccgtt acgcggcccc
     1321 aatcggcgtc gggagggatg ctcgggagtt cgatggaggg ctccggttcg tagttggcgg
     1381 cgaggcgccg ttcgaggtca aacgcgacga agcgattgag taggtgtggg gccacgctaa
     1441 cccagagttt ccggccttcg gtgtccataa tcacaccgtg ggtcgcgatc agcgcgtcga
     1501 tggcgttgcg atcgccgagc ttcaggtctt ttccgccaac gccttttcga tcgcgcagca
     1561 tgctggcaag cgtctcgggc gtcacggggc gcgccgcgtt gcgcaccagc tcgtcgccgc
     1621 gctcacggcg aggaagcgtg gaggtggagg cgcgcacgcg ctggttcttc ggatcggctt
     1681 gatggggccc ctcgaggtga ttggtgacgg cggccgcatc gcccaaagct tgtacgaaat
     1741 tgggcgctcc gggcacgcgc tccacgcgca cggcgtggcc ttggcggtcg gtgagcacga
     1801 tgatgtggct gaccagcggc gaagcctcgc cgaggatccg gatggcctcg gaagtatcgt
     1861 gcgcgcgccc caagacttca cgcagggtat gaacgacggg gcgcccttcc gcacgcggct
     1921 cgccggcacg cccgccgtgg acgacgacgg cgacgccctc gacattgatg ccactcacga
     1981 ccccaacgag cccgggccat gccaccgaag cgaacggcag ttttccgttc tcccgcacga
     2041 agaagacggc ctttttacgg tcgaagatag ggtcgacttc gaagtcgaag ttccgcgcga
     2101 gcagtgagcc cgaatgccca atttgtgccc ccgaaaatac gaatgtggtg cagccgatca
     2161 gcggtgaatg ttcgaaggag agcgcaatat cgtataacgc attcaggtat acgaagcgtt
     2221 gataagtcgg aaacatggtc cggaacgggt cgggctcaaa gccgagggcg ctggccgcga
     2281 tttcacgttg gcggtcctcg cccatgccga gatctacgtg tcgatatcgc acttgggcca
     2341 cgtccaggat caagcgccgc agcaagaagc taggtactga gtcttggaag tgtcccaaga
     2401 gaataccttc gttctcgcgc atctccggtt gcagtaggcg tgcgtgcgaa aaaccgattt
     2461 gctcggggct gccccgcagg tgcacttcga gcatgccggg gtgctggacc acgaacgacg
     2521 cgccgaagcg ccgcacgccg ggactgagtt cgagctttga ttgcgcagga acgtccaccg
     2581 cgcgaggttc gatcttgcac gcgcgcgtga cgacgttgtg cgcgagcagc gtgctcatac
     2641 cggcgaacaa cacaccgacc gcgagcttgg gcaccaagcg gcgcaaaaag ccaatcaccg
     2701 tggcgcccgc ttgaaggagg tcgtggcggc ggtgtacagg atgcgcgcca cgatgcgcgc
     2761 gggatctcgc acgctgtcga agtgggagac ccgctcctca gccgcgggat agatgacctc
     2821 caccgggacc tgttcgatcg ggactcccgc gcgggccgct ttgatgatca cctcggactc
     2881 gagctcgaaa ccgctgccgg ccagccccag agacgcaatg gcggccagcg gatagcggcg
     2941 caaaccgcat tgagtgtcgt tcagtcgctg gcgtgagaac ccggaaaccc aaaggttgga
     3001 gaagccattc gagaagcgat tggcccgcgg cgcgccggcc cgacgtagat cgcggatgcc
     3061 gagcaccaaa gcctcgggcg acgcggggtg acgggctaac gcgatcgcct gttctggagg
     3121 atgctgaccg tctgcatcga gtgtgaccac cacgtccccg gatagcgctg cggcataacg
     3181 aaagccggtg aggagggccg cgcctttccc gcggttaccg acgtgacgca gcacatgtgc
     3241 cccagcctgc tccgcgcgct gtgcagtggc atcgcgcgag ccgtcgtcta ccacgatcac
     3301 tgcgggccgc gttgtctcga gctcgggcgt aattctcgaa ataaggtcgg aaacgactac
     3361 gcccaccgac ttctcggctt cataggcggg aatgacgaac gcggtcttca tgcgcttgcc
     3421 cgggagcaca gcacaacttg gatctcggcg cgagccgacg cgatgctaac ctgggcccgt
     3481 gattgaggac tcggaactca ggagagagct ggcaagccaa gcccgccgcc agctacggac
     3541 ccgcatgcgg gcgctacgcg ccgcgcaccc cgaggccgcg ctcgagctgc gaagtgcgcg
     3601 aatccgcgcg tatctcgagg cgttgccgga attcaagagc gcccagggcg ttgcgctgtt
     3661 ctggccattg acgagcgagg tggatttgcg ccccctggac gtgaccgctc gttcgctggg
     3721 caagcgcgtt tactacccgt tcctcgagcc tggcccagag cgtagtctga ccgggttcgc
     3781 gcgcgtggac gacatctcca ccttggcccc gcgcggacag cgatttttcg agccccaggc
     3841 gggatcgccg cacgcaagcc gcggcgagat cgacttggtc gtagttccgg cgctcgcggt
     3901 gagcaccacc gggcatcgtc taggttacgg gtctggattt tatgacgcaa ccttgcccga
     3961 cttcattccg ccggcgcaag ccgtggtggt agcttacgat ttccagttgt tggctgaact
     4021 cccgacgttg caccacgacg tggcctgcca cgtcgtcgtt acggacgccc gaacgctgcg
     4081 cgtgccatcg tcggcgtgag ctgtttcgag cgagccatca actcgtgacc cagcgaaagg
     4141 tcaggttgat gcgcgccccg agcggagcgg ccgtcttggg gaccgcatga cggaaaacgt
     4201 gttggaaggt gccgcccatc caaagcaggc tccccgagct cagcacgagc tccccaccgg
     4261 aaagttgctt ctcgaagcgg cgcttggggc gatataagaa gcggcgcgcc gcacccaagg
     4321 acacactcgc gatctccggg tttggcccca gctcgggttc gtcatcggca tggaagccca
     4381 tgctgtctcg tccatctcgg tataagttga gcagcaccga gttgaagcgc gaaccggtgg
     4441 cgagttcgat gcggtcgcgg atctcgagca gggccgggag ccatggccga gcagctacgc
     4501 gccggccact gtacgtgtag tccgcgtcgc caaaccaggc cgacagccgc ggctccagca
     4561 ccttcttgcc gaagaacgag agctcgccct gttgccaagg cactcgttcc gagagctccg
     4621 cctggagtcg ggacgcttgc tcgggggaca ggaatgcggc ctcgtagcgc aacactgcgc
     4681 ccgcgcccaa tgacaccggc tctgcccgca atgacgtggc tgaatctcca gccgctcagc
     4741 ggcgcttgcg cgcgttcggt ttttggcact cggcgcagac gccatagagc tcgtgcttgt
     4801 gctgcttcac ttggaagccg tagcgcgtcg caacacgctg ctgcagctcc tcgatcagag
     4861 gctcctcgaa ctccgtgatg ctcccgcact ccaagcaaat caggtgatcg tggtggtggt
     4921 cttcgtcaac gagttcgtag cgggtgaagc cgtccccgaa cttgtgctct tgcacgatgc
     4981 cgctatcggc caggagcttc atcgttcgat acacggtcgc gtaaccaacg cgcgagtcca
     5041 ccgttcggac ttggcggagc agaccgtcga tagtgatgtg ttctcccgcg tcgaacagcg
     5101 tgtcgatgat cagtcgccgc tgctccgtcg agcgcaaccg gcgcttggcc atgtacgtgt
     5161 ccagatcacg acgtagtcga acttgatcag gattcttgtg gccgcggaca gcctgagtca
     5221 cgctcttcac gctcaaggta ttttccaccc ttgggctcgc gtcaagcacc ggcgcaccga
     5281 cgtcagcgac gtgccctgag gcgcaccagg gcgacgccgc cgccgagcaa ggccccgatc
     5341 ggagaccacg ccggcgcggc caagcccaaa cctgccacaa tcgcaaacgc aacggcgggg
     5401 cccagaatga gcgccaaaga cagccgggcc tcgcgccggg cctcgtgcgc cagccgaatt
     5461 gcgaaggcag cgtctcgaac gtcatcggag gccagttgca cattccactc ggaagaggtg
     5521 cttccagcgg ctccgagcgc gatcgatacg tctgccgcgg acaaggccac ttcatctgct
     5581 tggctgcggc ccacgacggc cacgatcgcc ccgccgtccg ccaaacgacg aatggcgtca
     5641 ccgcgttctg ccggcgggat ctcggggcgg atgtggtcga tgtccaaagc ccggcccaaa
     5701 gcctcgcagg tttcccgcgc atctcccgag agcaacacgg gctccacccc gatgtccaag
     5761 gcgtgttgga cggaggcccg cgcgccggga cgcaacccat cctgtagtcc cagtaagcca
     5821 atcaagcggc ccccgagcgc aaccaagagc acgctgcgtc ccatcgcctc gagcgcggta
     5881 atgcggctct cgacggcagc gatgctgacg tgctccttga gcatgagcgc acgactgccg
     5941 acgacgagtg cctgaccgtt ggatgcgacc gccgtcaccc ccaaccccgc ttggggtgta
     6001 gggctgcggg tgccgtccgg acgcacgccg cgcgcacgcg ccgcgcgtaa gatcgcggtg
     6061 gagaccgcgt gctgggagcc gctctcggcg ccggcaacca gagacaacac gtcgctcggc
     6121 cggtggccga agaaggcgtc gatatccgcg acctctggct ctccgagctg caaggttccg
     6181 cgcgcacaga aggcgagcat gctcaccttg ccggcgcgat cgaaggcgtc ggccgtgcga
     6241 aacgcgatcc cgcgccgcag accctcgagg atggcccgcg ccacgtactg gctccccgcc
     6301 gcggcgagga ttgggcaggt caacgccgcg tgaatggaga cgcagagcac tccaatctcg
     6361 agcgggtcca agttggcggc aaaggcagcc agagccgcgg tgcccgtgat gaacggcgca
     6421 atgcgctcca cgatcaggcg gcccacgcgc gccgtgctgg cgtgcaagtc cgctcgtcgg
     6481 cgggggtcgg tcgagagtcg aagccacgcg cgatcggggc ccgcccagct cacgatggcc
     6541 cggagccgac cttcgaccac gcgcgcaccc gcg
//
LOCUS       DASTKU010000593        11732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1677906, whole genome shotgun sequence.
ACCESSION   DASTKU010000593 DASTKU010000000
VERSION     DASTKU010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11732
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677906"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(402..986)
                     /locus_tag="VFQ61_20790"
     CDS             complement(402..986)
                     /locus_tag="VFQ61_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956952.1"
                     /translation="MPEGWPPDGSSLEDYDVAGELCGNRILYGAPFSDEALFGLRGTV
                     DGEPAAMEPSDQNLGDCSKPCVRLGVLADPIPDTADTPKARLRLQLAQLSVRLDSIGP
                     WIATFQVGRPASKVTSDACKLLVSTTGPQPAQAEGTLVTTFVLAPDGMERGNAVAVLD
                     ATLAFPDPAFGLVSKQLHLELALPWTYRIATPRP"
     gene            1633..2220
                     /locus_tag="VFQ61_20795"
     CDS             1633..2220
                     /locus_tag="VFQ61_20795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2378 family protein"
                     /protein_id="HET9956953.1"
                     /translation="MAHMPSPFVDPPWNIALDVAMCLASIPQHAEIAGMYCNALVAAG
                     KRKGVTFSTARERYVQFNYYPVSEFARMLVEATDRLYPALSKREALRKLGQGGPKAFA
                     GSLLGKVVLGSAEGVHATVLAVAKTYEVNTRPSRCWVKETRPQSMIVSLENVYHFLDS
                     HHVGVFEGTLRFAGVNGRVRIASHGRTAADLLLEW"
     gene            complement(2472..3398)
                     /locus_tag="VFQ61_20800"
     CDS             complement(2472..3398)
                     /locus_tag="VFQ61_20800"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HET9956954.1"
                     /translation="MSVTAASRPSSKPQRLAASFMSGAVFNLRAMFVAMSFGGAAAGC
                     ASAPSPLQPAQFGSVGVPNRGVLTGALELPASGAGYVRYRTRGQHHFGVPRLVRALER
                     AAAGVQALAPGEPLVIGDLSARDGGRISGHNSHRTGRDVDLLYFATTPGGVPVRSPGF
                     IPFEADGLAYDRVRSNFVRFDVDRVWLLIRGLLTDPEISVQFLFMSRPLETQVIDYAI
                     ARGEPLPLVYHAAATLTQPTDSLPHDDHLHVRVACTPEELLTGCSGGGPWWEWFPKLP
                     PPPELHELEYLAIGSEDPLIEAPLPDTASASR"
     gene            complement(3395..6034)
                     /locus_tag="VFQ61_20805"
     CDS             complement(3395..6034)
                     /locus_tag="VFQ61_20805"
                     /inference="COORDINATES: protein motif:HMM:NF039580.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET9956955.1"
                     /translation="MAGPELTQTYEPNPILGAVFRRLFEHIRVDDAWAQKIRQLSQRG
                     RVVYVLRNLNIVDFLALDYLTRRHDLPRIRFVNDLGLWILNPLGKGWLNAILPKRHVQ
                     PQDELEDALSRGGSAALFLKRPPGVLDVAAGATRGLGLKDGDELVRALFDLQRRHSEP
                     ILLVPQLFLWSKVPDKLGTDTRDLLLGLGEWPTPLRAVAQFFYNYKHVSLRAGEPLDL
                     QAFLAESEGQSDTVTARRLTYAMLRRLERERRAVTGPAEKPSERVRLEIVQSPRLRSV
                     IDDLVDEHTDRYTLQTRALGMLEELQARPDRTTVKVLELVFDRLFQRIYAGVEYDAAD
                     IERLREAAKRGTLVLLPSHKSHIDYLILSYIFNMENLPLPIIAAGDNLNFFPAGGILR
                     RGGGFFIRRSFRGDRLYAAVVDAYVRRLIRDGYPIELFLEGGRSRTGKLVPPKFGLLS
                     MIVDAALAVPSTPSYFVPVSIGYERVIEGEAYERELKGGEKAKEDAAGLLKSGNLLRH
                     RYGRINMQVGQILSLSEITSELSVDTSQLSPVKRRNLVTRIGNRVMDEINRVTAVTPG
                     ALTALVLLSHDQRGLSHTELVGRSRRLLEVLRQLGARTSPALVTPAGNLRTESLREAA
                     QLFVDGDLVELHHPDREPEGGRAKASHDAFYVVPHAKRLLLDTTKNIIVHFFVERALV
                     ALALLSLAQAAGDPTATEGIAREDVRERVRELSRLFKHEFRFRADAPFEQIFEETLQQ
                     MTEMHELATEGDRLRAGPGRDGCGGYDVLLGYAAMLKNFLEGYRIAARSLRELLERSQ
                     ADKEMLKKALSLGQHLFLEGDLSRSEAVSRPILSCAYAAFADQGYVIHRESKYHPGPE
                     LTGPAAIEALEQRIRNYTLERKA"
     gene            complement(6119..6385)
                     /locus_tag="VFQ61_20810"
     CDS             complement(6119..6385)
                     /locus_tag="VFQ61_20810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956956.1"
                     /translation="MPSIPSVEPLGAPNGSDTFVGAERSAPAGRSPLGTAVLLRQAPP
                     TTTKLVNAHAAGNPMSARQVEAMRRQYSQLADPASMMARTDGSP"
     gene            6390..7718
                     /locus_tag="VFQ61_20815"
     CDS             6390..7718
                     /locus_tag="VFQ61_20815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956957.1"
                     /translation="MTPSALACRALSNKSASRAAPSPSSVTTSKAPNADAAQKVEGVW
                     LGTASALAAPLDPDADEANVERPFSVELGTARGAFNQFAVPALETRQAATFATITLVD
                     SDVGTGRSVELGRVYGDVEPPALTTWEAGFAVLSADHDASALALRLYTLSAPFDGSGL
                     KKGPEIRSVRRDTQGFALEAHGHEALAVYSKLEKGKGTLGLSRFDLRALTAIGGSTLA
                     PHGGEGDQESPRLIAREGGYYLAWLLRTFVKPRVAPRLAARAAKDGDSDRPEEPVLEE
                     GPSAIEIVPLDGAGVAVASPRRVTPPEAHVTSFDMAPTRDSGALVVYRDERDGPGLDR
                     ANIEAIWVRPDGSSTARTWELSESVGLPSLLVDPGLGKDQPFIAVSVQLEAETRLAHW
                     DETTAGLLEPSAHEALGNRELLARVGERYLTSEVRGSQRHLGVISCRFLR"
     gene            7762..8313
                     /locus_tag="VFQ61_20820"
     CDS             7762..8313
                     /locus_tag="VFQ61_20820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HET9956958.1"
                     /translation="MSQPTKILVPLAEGFEEIEAVTIIDVLRRAGVDVTTAALTGQTV
                     RGAHGIQVVADCTIDDVRESDFQGVVLPGGMPGSKHLREDPRILMLLRLFAQTGRLTA
                     AICAAPTALAAAGLLTGRRATSYPGATLPGADYSEDKVVEDGPFMTSRGVGTALEFAL
                     ALVRRLKGPAAASEHATKVLFQP"
     gene            complement(8377..8955)
                     /locus_tag="VFQ61_20825"
     CDS             complement(8377..8955)
                     /locus_tag="VFQ61_20825"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET9956959.1"
                     /translation="MRTKARRVQILLFDEVELYDVAAPAEILSVAGRHWNFRPYKLEL
                     IAEQTGFVTTRNQLRLEASYALAQAEPAEILLIPGGFGARRFAENANRLAEVARLSEG
                     AELIGCIGNGAMVIESAGMFPKNETLPALASAPPPRRGLREEGRVLQAFSTGDALELG
                     LAIVRRTLGQKLVTWVCGELGLETRERLEFRY"
     gene            9063..10163
                     /locus_tag="VFQ61_20830"
     CDS             9063..10163
                     /locus_tag="VFQ61_20830"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET9956960.1"
                     /translation="MGELAFELHRKPQPLSDVERADVMKNLGFGRVFTEHMVTLRYSV
                     EAGWHAGRIEPYAPLTLDPAASVLHYGQAVFEGFKAYRQPDGGIASFRPDAHARRFAA
                     SARRLAMPELPLDAFVAAADALIRAEQHWVPSKPGESLYLRPLMIATEAALGVRPARE
                     YTFLLFGSPSGNYFPQGIKPLSVWLCKDFVRAAPGGTGEAKCAGNYAASLVAQQRALE
                     QGCQQVIWLDACERRYVEEMGGMNLFFVYEKAGRVRLVTPRLTGSLLAGITRDSILKI
                     GAELGYEAEEATISVEDWEQGVQSGELTEVFACGTAAVITPVGQVKFEGGAWPIHGGE
                     SGPVALKLREHLLAIQHGERADVYGWMHRIAL"
     gene            complement(10225..10701)
                     /locus_tag="VFQ61_20835"
     CDS             complement(10225..10701)
                     /locus_tag="VFQ61_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956961.1"
                     /translation="MSLPTAIAVAQHSPHEIASSVTLGGEHESDATLYLRFPAGSADR
                     ALLLAWLVLDLRESTRSSEPVTLEIGRARGPFSSGGLRPTEQPGLTPPLVEYRIESGA
                     TGPLRVNVTELVQYFERHAQRAPILAIRARNTRGAGVTLSTGVDGGRAPRLDAYWR"
     gene            complement(10900..>11732)
                     /locus_tag="VFQ61_20840"
     CDS             complement(10900..>11732)
                     /locus_tag="VFQ61_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956962.1"
                     /translation="GRHASFRQLTARVAGPDGHTREVNLEPTGAGQYIAHLPVNQPGS
                     YLASLLDDSAQSLGTTGAVLSRGDELRPTGTDRALLRRISELSSGKVRDTLAGIFNDR
                     DARRFAYDALSGWLAAMAALCLLGSVAIRRIHLPPLRWPGREHTAAKPGTYAGNSDSA
                     AYANQGLEALRAVRERLRARAPMAADAQAAGGAPTASKGENDTLLTAAPKETTPARFV
                     LGPEAPAARSSMPESPGTSQANEPVTPSVAHAAPAPTPSPTRTKSAAEILLERRRARR
                     "
BASE COUNT         2011 a         3518 c         4056 g         2147 t
ORIGIN      
        1 cgccgacccc gtagcgcgcg aagaaccaaa aaatggatcc aaacatcaac ccgagcagga
       61 tggccatcgc gccgcagcct atcaccactc gagcgagccc cactactgcg gcgtccgagc
      121 accgcctgca aacgcggctg gcgcactcgc tcgaccccgc agcaagcggt gcccgagccg
      181 aatcgtgtgg cgctcgacat gcgtcagctc gagcatcttt gggattgccg cgcgggagac
      241 aacgggcgcg ccgcgcgacc ccaatcccgc gagctcagcc gccgccccac gcaaagcggc
      301 tcagcaccgc gcctgcggca tgaaacaacg ccagcgcatg aaaggccagc ggccgaacaa
      361 cgcactcgag aataaagaca gccaaggccg cccaccggcc gtcacggcct gggagtggcg
      421 atgcgatagg tccacggcag cgccagctcc aaatgcagtt gcttgctcac cagcccaaag
      481 gcagggtccg ggaaagcgag cgtagcatcg agtacagcga ccgcgtttcc tcgctccatg
      541 ccgtccggcg ccagaacaaa ggtggtgacg agtgtccctt cggcttgcgc cggctgcggg
      601 cccgtcgtgg agaccagcag tttgcatgcg tcgctcgtta ccttgctcgc ggggcgaccg
      661 acttggaaag tagcaatcca gggcccaatc gagtccaagc gaacggatag ctgcgcgagc
      721 tgcagccgaa gacgggcctt gggcgtgtcg gctgtgtcgg gtattgggtc ggccaacact
      781 ccgagtcgta cgcatggttt cgagcagtcg ccgaggttct ggtctgacgg ctccatggcc
      841 gcgggttcac cgtcaactgt tcctcgcaac ccgaacagag cttcgtccga aaacggcgca
      901 ccgtatagga ttcggttacc gcagagctcg ccggcaacgt cgtagtcttc gagggagctt
      961 ccgtccggag gccacccctc aggcaaggtc ggcttgggtg tattgggctc aggtgtaccg
     1021 ggctcaggtg tcttgggctc aggtgtaccg ggctcaggtg taccgggctc aggtgtaccg
     1081 ggctcaggtg taacgggctc aggcgtactg ggctcgggcg attgggatga cggagcgggg
     1141 tcgtgcttca cgcgaccgcc gcacgcgagc gttatggacc cgagtgcgaa tgtgcacacc
     1201 agagccgttg catgcgcaag cgttgctgcg cggggccggc gcggtgtggc gacggatgcg
     1261 gggcggaaga gctctgcgcc aaggtgggag tgcgtcgagt attcgcgcac gttgcgtttt
     1321 ggcccgatcc accccgcgac cgatgcgaag cgagctcgca agttacgact cgagatcatg
     1381 aaacggcagc acagcatcgt ggttccggaa aacgtcagcg cgtaggcggg ggtggatcaa
     1441 gcggatttcc cgcaatgcac tgggcgtagc atggcgccga ccgttgtcgc gcgtacgggg
     1501 gcgtgtgcga gtgtaacgac gctctcgcag ggctggttcc gcgtggctgc gcagttggtg
     1561 taccctcgat ctcggtgcat tagatggcat catccaaggg tggacggaat tcgagaagag
     1621 aggaggcgac gcatggcgca catgcccagc cccttcgttg atccgccttg gaacatcgcg
     1681 ttggatgtgg cgatgtgctt ggcctcgatt ccccaacacg ccgagattgc cggcatgtat
     1741 tgcaacgcgc tggtcgcggc gggcaagcga aagggcgtaa cgttctcgac ggcgcgagag
     1801 cgttacgtgc aattcaatta ttacccggtc agcgagttcg ctcgcatgtt ggtcgaagcc
     1861 accgatcgcc tctatccagc gctcagcaaa cgcgaggcgc tccgcaagct ggggcagggc
     1921 gggcccaagg cgttcgcggg gtcgctgctc ggcaaggtgg tgttgggatc cgccgaaggg
     1981 gtgcacgcga cggtgctcgc cgtggccaag acctacgagg tcaacacgcg gccgagccgc
     2041 tgttgggtga aggagacgcg gcctcagtcg atgatcgtga gcctcgaaaa cgtgtaccac
     2101 tttctcgact cgcaccatgt cggggtgttc gagggcacgc tgcgtttcgc cggggtgaat
     2161 ggtcgcgtgc gcattgcgag tcacggtcga accgctgcgg atctgctgct ggagtggtga
     2221 ggcgctattt cttgggaggg tctgtttgct gtgtttcttg ggagggactg ttccagaccc
     2281 tccccccgcc gggagtgaat cccggcgggt cccccctccc aaggcgcgtt gcgctgcgcg
     2341 cggggcccca gccccgcttg cgaggacgtc ataaaccgat ggggttgcaa tagtcttttc
     2401 tccgcctccc aaggcgctat gcgctgcgct cggggcgtgt tggctgggtg aaaggtcgga
     2461 gtggatgcgg gttatcggga ggcggaggcg gtgtcgggga ggggagcctc gatgagcgga
     2521 tcttcgctgc caatggccag gtattcgagc tcgtggagct cgggaggggg aggcagtttg
     2581 ggaaaccact cccaccaagg gcccccgccg ctgcagccag tgagtagctc ttcaggggtg
     2641 cacgcgacgc ggacgtgcag atggtcgtcg tggggcaggc tgtcggtcgg ttgggtcaag
     2701 gtggcggcag cgtgatacac gagtggaaga ggctcccccc gcgcgatagc gtagtcgatg
     2761 acttgggtct cgagtgggcg actcatgaac aggaattgca cggagatttc gggatccgtg
     2821 aggaggccgc ggatcaggag ccagactcgg tccacgtcga atcgtacgaa gttcgatcgg
     2881 acacgatcgt aggcgagccc gtcagcttcg aaggggatga atccagggct gcgaaccggt
     2941 acgcccccgg gggtggtggc gaagtagagt aagtccacgt cgcgacccgt gcggtgcgag
     3001 ttgtggccgg aaatgcggcc gccgtcgcgc gccgagaggt cgccaatcac gagcggttcg
     3061 cccggcgcta gcgcttgcac gcccgccgcg gcccgttcca aagctcggac caatcgcggg
     3121 acaccgaagt ggtgttgtcc tcgggtccga tagcgaacgt agcccgcacc actcgcgggc
     3181 agctcgagcg cgccggtcaa gactccccgg ttcggcacgc caacgctgcc aaactgtgcc
     3241 ggttgtaaag gtgaaggcgc cgaagcacag cccgccgcgg ccccgccgaa ggacatggcc
     3301 acgaacatgg ctcggagatt gaacacggcc ccgctcatga aagacgcggc gagcctctga
     3361 ggcttggagc tcgggcggct cgccgcagtc acgctcatgc tttgcgctcc agggtgtagt
     3421 ttcggatgcg ctgctccaag gcctcgatcg ccgcggggcc ggtgagttcg ggtccggggt
     3481 gatacttgct ctcgcggtgg atcacgtagc cctgatccgc gaaggccgcg taggcgcagc
     3541 tgaggatcgg gcgacttacg gcctctgaac gcgacaggtc gccttccaag aacaggtgct
     3601 gccccaggga gagggctttc ttcagcatct ctttgtccgc ttgggagcgt tcgagcagct
     3661 ctcgtaacga gcgtgcggcg atgcggtagc cttcgaggaa gttcttcagc atggcggcgt
     3721 agcccagcaa gacgtcgtaa ccaccgcagc cgtcgcgtcc cgggcccgcg cgcagccggt
     3781 ccccttccgt cgcgagctcg tgcatttccg tcatctgctg cagtgtctct tcgaagatct
     3841 gttcgaacgg cgcatctgca cggaaccgga attcgtgctt gaaaaggcgg gagagttcgc
     3901 gcactcgctc gcgcacgtct tctcgcgcga tgccctcggt cgcggtcgga tcccccgcgg
     3961 cttgagcgag cgacaacaag gcgagcgcga cgagggcgcg ctctacgaaa aagtgaacga
     4021 tgatgttctt cgtggtgtcg agcagcagcc gcttggcgtg cggcaccacg taaaatgcgt
     4081 cgtggctggc tttggcgcgg ccgccttccg gctcacgatc ggggtggtgc agttcgacca
     4141 agtcgccgtc gacgaacagc tgcgcggctt cgcgcaatga ctcggtgcgc aagttgccag
     4201 cgggggtgac gagcgccggg ctggttcgcg ccccgagctg tcgaagcact tcgagtaagc
     4261 gacggctgcg gccgaccaac tcggtatgcg agagccctcg ctgatcatgg ctcagcagca
     4321 cgagcgccgt gagcgcgccg ggcgtgaccg ccgtgacgcg gttgatttcg tccatcacgc
     4381 ggttgccaat gcgtgtgacg aggttacgtc gcttgaccgg cgacagctgg ctcgtgtcca
     4441 cgctgagctc gctcgtgatt tcggagagcg agaggatctg gccgacctgc atgttgatgc
     4501 ggccatagcg atgccggagt aggttgccgg atttcagtag ccccgctgcg tcttccttgg
     4561 ccttctcgcc gcccttgagc tcgcgttcgt aggcttcgcc ttcgatcacg cgttcgtagc
     4621 cgatgctgac gggaacgaag tagctcggcg tgctgggcac cgccaacgcg gcgtccacga
     4681 tcatgctgag cagtccgaat ttcggaggaa ccagcttacc ggttcggcta cgcccacctt
     4741 ccaagaacag ctcgatcggg tagccatcgc ggatcagccg gcgcacgtag gcgtccacta
     4801 cggccgcgta aagccgatcg ccgcgaaaag atcgacgaat gaagaagccg cctccgcgcc
     4861 gcaggatgcc gcccgccggg aagaagttca ggttgtcgcc cgccgcgatg atgggcagcg
     4921 gcaggttctc catgttgaag atgtagctga ggatcaaata gtcgatgtgg ctcttgtggc
     4981 tcggcagcaa aaccagggtg ccgcgcttgg ccgcttcgcg tagtcgttcg atgtcggccg
     5041 cgtcgtactc cacgcctgcg tagatgcgtt ggaacaagcg atcgaagacg agttccagaa
     5101 ctttgaccgt ggttcgatcc gggcgggcct ggagttcctc gagcatgccg agggcgcgcg
     5161 tttgcagcgt gtagcggtcg gtgtgctcgt ccaccaggtc atcgatcacg ctgcgcagcc
     5221 gtgggctctg cacgatctcg agtcgcacgc gctcggaggg cttctccgcg gggcccgtca
     5281 cggcgcggcg ctcgcgctcg aggcgccgca gcatggcgta ggtgagccga cgcgcggtga
     5341 ccgtgtctga ctgaccttcg ctctccgcca ggaacgcctg taaatcgagc ggttctccag
     5401 cgcgtaagga gacgtgcttg tagttgtaga agaactgcgc gaccgcgcgc agaggcgtgg
     5461 gccattcccc gagcccgagc aggagatcgc gcgtgtcggt tccgagtttg tccgggacct
     5521 tcgaccacag gaacagctgg ggcacgagca ggatcggctc cgagtgtcga cgctgcagat
     5581 cgaagagagc gcgcaccagc tcgtctccgt cttttaggcc caagccgcgg gtggctccgg
     5641 cagctacgtc cagcacgccc ggtggacgtt tcagaaacag tgcggccgag ccgcctcggc
     5701 tgagcgcgtc ttcgagctcg tcctggggct gcacgtggcg cttgggcagg atggcgttga
     5761 gccaaccctt acccagcggg ttcaagatcc aaaggcccaa atcgttgacg aagcggatgc
     5821 gcggcaggtc gtgacgccgg gttaggtagt cgagcgccaa gaagtcgacg atgttgaggt
     5881 tgcggagcac gtacacgacg cgcccgcgct gcgagagctg gcgtatcttc tgcgcccatg
     5941 cgtcatcgac gcggatgtgt tcgaacaggc gccgaaacac ggctcccaag attgggttgg
     6001 gctcgtaggt ctgagtgagt tcggggccgg ccatggggag attgagcttt ccgctctgtg
     6061 ggatcgagct tgctgctccg tagatggagc ctgctgctcc gtagatcgag ccgcggcttt
     6121 atggggagcc gtcggtgcgc gccatcatac ttgccggatc cgcgagctgc gagtactgac
     6181 gccgcatggc ttcgacttgg cgcgcgctca tcgggttacc agctgcgtgc gcgttgacga
     6241 gtttggtggt cgtaggtggc gcttgtcgca gcaaaacggc tgtaccaagc ggcgaacgcc
     6301 cggccggagc ggatcgctct gcaccgacga aagtgtcgga gccattgggc gctccgagcg
     6361 gctcgaccga aggaatagag ggcaatgcag tgacaccgtc cgcgctcgcg tgtcgcgcgt
     6421 tatcgaacaa gagtgcgtcg agagcggctc cgtctccgtc gagcgtaacg acttcgaagg
     6481 ccccgaacgc tgacgcggcg caaaaggtgg aaggcgtgtg gcttggaact gcgagcgcgc
     6541 tggcggcgcc gttggatccc gatgcggacg aagcgaacgt ggaacgaccg ttctcggtcg
     6601 agctcggcac cgcgcgtggg gccttcaatc aatttgcggt gccggcgctc gaaacccgcc
     6661 aggccgcgac gttcgcgacc atcaccctcg tagatagtga cgtagggacc ggacgcagcg
     6721 tggagcttgg aagggtgtat ggggacgtgg agcccccggc gttgacgact tgggaagctg
     6781 gattcgcggt gctctcggcg gatcatgacg cctccgcttt ggcgttgcgc ttgtataccc
     6841 tgtccgcgcc gttcgacggc tcgggtttga agaagggtcc ggagattcgc tcggtgcggc
     6901 gcgacactca gggcttcgcc ctggaggcgc acggacacga ggccctggcg gtctattcga
     6961 agctcgagaa gggcaaaggc actctcgggc tgtcgcgctt cgacctgcgc gcgctgaccg
     7021 cgatcggtgg ctcgactctg gcgccgcacg gcggtgaggg ggatcaggaa tctccgcgct
     7081 tgattgcgcg cgaagggggc tactacttgg cgtggttgct gcgcaccttc gtcaaaccgc
     7141 gggtagcacc gcgtctcgcc gctcgcgccg ccaaagatgg agactccgat cgtccggaag
     7201 agccggtgct ggaggagggg ccgagcgcca tcgaaatcgt gcccctggac ggggcggggg
     7261 tcgccgtggc gagcccgcgc cgcgtgactc cgccggaggc acacgtgacg tctttcgata
     7321 tggcgccgac tcgggatagc ggcgcgctcg tggtctatcg agacgagcgg gacgggcccg
     7381 gcttggatcg ggccaatatc gaggcgattt gggtgcgccc ggacggctcc agcaccgcgc
     7441 gcacgtggga actttcggag agcgttggct taccgtcgtt gctggtggat ccgggcctgg
     7501 gcaaagacca gccgttcatt gcggtttccg tgcagctcga ggccgagacg cgtctcgcgc
     7561 attgggatga aacgacggcg gggctgctcg aaccgtcggc tcacgaagcc ctcggcaacc
     7621 gcgagctcct ggcccgggtc ggcgagcgtt atctgacttc cgaggtgcgt ggcagtcagc
     7681 gacacttggg ggtcatttcg tgccgattcc tgcgctgacc acgcgaccat aagggtgtag
     7741 tactctcgac ggaggtgagc cttgtcccag cctacgaaga tcttggttcc gctcgctgaa
     7801 ggtttcgagg agatcgaggc cgtgacgatc atcgatgtgc tgcgccgcgc gggcgtggat
     7861 gtcaccacgg cggcgcttac gggccaaacc gtgcggggcg cccacggcat tcaagtcgtg
     7921 gccgactgca cgatcgacga tgtgcgcgag agtgattttc aaggcgtggt tttgccgggc
     7981 ggcatgcccg gatccaagca cctccgggaa gatccccgaa tcttgatgtt gctgcgcttg
     8041 ttcgcgcaaa cgggcaggct gaccgcggcg atttgtgccg ctccgaccgc gctcgcggcg
     8101 gcgggcttgc tgactggacg acgcgcgacg agctatcccg gcgcgacgct gccgggcgcc
     8161 gactatagcg aggataaagt ggtggaagac gggccgttca tgaccagtcg tggggtgggg
     8221 acggcgttgg agttcgcgct ggctctggtg cgtcgactca aaggtccggc cgccgccagt
     8281 gagcacgcga cgaaggtgct gttccagccc taatttctag ccggcgacaa atctcacttc
     8341 gcgctacgca gcggtcgaga aacgcgtgct ttgcgctcag tagcgaaatt ccaaacgttc
     8401 acgcgtctcg agtccgagct caccgcaaac ccaagtcacg agcttttgcc cgagggttcg
     8461 ccggacgatt gccaagccca gttcgagcgc atcgcctgta gaaaacgcct gcagaacgcg
     8521 cccttcttct ctcagacctc gccggggagg cggcgcgctc gccagcgccg ggagcgtttc
     8581 attctttggg aacatcccgg ccgactcgat gaccatcgcg ccgtttccga tgcatccgat
     8641 aagctccgca ccttctgaga ggcgtgcgac ttcggccaac cgattggcgt tctcggcaaa
     8701 gcgacgcgcc ccaaatccgc caggaatcag caagatctcc gcaggttcgg cttgggctag
     8761 cgcgtagctc gcttcgagac ggagctggtt gcgagtggtc acgaacccgg tttgttccgc
     8821 gatcagctcg agtttgtagg gcctgaagtt ccaatgtcgg ccggcgacgg acaggatctc
     8881 cgcgggagct gccacgtcat acagctcgac ctcgtcgaag agcaggatct gaactcggcg
     8941 cgccttggta cgcatgcaac tcaactggtt ctacctcagg cgggccgcga cgtcgctgaa
     9001 tcactcatgc agcctgcgcg caaagtctga cgctttgtat gctatgccca ggctaaccaa
     9061 tcatgggtga gcttgcgttc gaactgcacc gaaagccgca gccgctgagc gacgtggagc
     9121 gcgcggatgt gatgaaaaac ctcggttttg gccgggtttt caccgagcac atggtgacgc
     9181 tgcgttactc tgtggaagcg gggtggcatg cgggccgcat cgagccctat gcgcctttga
     9241 ccctcgaccc tgcggcctcg gttttacatt acggccaggc cgtgttcgaa ggattcaaag
     9301 cctatcggca gccggacggg gggatcgcaa gctttcggcc ggatgcgcac gcccggcgct
     9361 tcgccgcgtc cgcgcggaga ttggcgatgc cggagctccc gctcgacgct ttcgttgccg
     9421 ccgcagatgc cttgattcgc gcggagcagc attgggtccc gtcgaagcca ggtgagagcc
     9481 tgtacttgcg gccgttgatg atcgccaccg aggctgctct cggcgtacgg ccagcacgtg
     9541 agtacacgtt cctgctgttt gggtcgccgt cggggaatta tttcccgcag ggcatcaagc
     9601 cgttgagcgt gtggctctgc aaagatttcg tgcgcgcggc gcccggcgga accggtgaag
     9661 cgaagtgtgc agggaactat gctgcgagtt tggtcgcgca gcagcgggcg ttggaacaag
     9721 gatgtcagca ggtgatttgg ctggatgcct gcgaacgccg ttacgtcgag gagatgggcg
     9781 ggatgaacct gttcttcgtc tacgaaaagg cggggcgcgt gcgcttggtc actccgcgtt
     9841 tgacgggctc tctgctcgcg ggcatcaccc gagacagtat cctgaagatt ggcgcagagc
     9901 tcggttacga agccgaagag gcgaccatca gcgtggaaga ctgggagcag ggagttcagt
     9961 cgggcgaact cacggaagtg ttcgcttgcg ggacggcagc ggtcattacc ccggttggcc
    10021 aagtcaagtt cgagggtggc gcatggccca tccacggtgg agagtcaggg ccggtcgcgc
    10081 tcaagctgcg agagcacttg ttggccattc agcacgggga aagagcggac gtttacggct
    10141 ggatgcatcg tatcgcgctg tgaggcaggg tgtcgatcgt gcgcgggtgg ctcgattcga
    10201 gctgcccgcg ggctcgtatc gggctcatcg ccagtaggcg tccagacgcg gggcgcggcc
    10261 gccgtccacg ccggtactga gcgtgactcc ggcgccgcga gtgttgcggg cccgaatcgc
    10321 caagatgggg gcgcgttggg cgtgccgctc gaaatactgg acgagctccg tcacattgac
    10381 gcgtagcggg ccggtcgcgc cgctctctat tcggtattcc acgagcggtg gggtgagtcc
    10441 gggctgctcc gttggcctca agccaccgct cgagaatggc ccgcgtgctc gcccgatttc
    10501 gagggtcacg ggttcgctac tgcgagtgga ttcccgcaag tcgagcacca accaagctag
    10561 cagcaaggcg cgatcggcgc ttccggccgg gaatcgtaag tacagagtgg catcgctctc
    10621 gtgttcacct ccgagggtga cgctggacgc aatttcatgc ggcgagtgtt gcgctaccgc
    10681 gatggcggtg gggagagaca cctggcgttg cgcgtccgaa gggagagcgg gtgaagcgca
    10741 gggggccaat gcggccgcgc cctgcgcgca cggctgttgt gcatgacagc cgagcagcag
    10801 gagactggtt agcgcgccaa gccggagttt cccggctgtg ccgattctag cggccgtgcc
    10861 cgtactaacg tcagggggtg gatgcgggat catggtcgtt tagcgtcgcg cacggcggcg
    10921 ctccaacaga atctcggcgg cactcttggt gcgggttggc gaaggcgttg gggcgggcgc
    10981 cgcgtgcgct accgacggag tcacgggttc attggcttgg gacgtacctg gtgattccgg
    11041 catagaacta cgtgctgcag gggcctccgg ccccagaacg aagcgtgctg gtgtcgtctc
    11101 tttaggagca gcggtaagaa gggtgtcgtt ttcgcccttc gacgcggtcg gggctccccc
    11161 tgccgcttgc gcgtctgcag ccattggcgc tcgggcacga agtcgctctc gaacggcacg
    11221 tagcgcctcg agcccttggt tcgcatatgc ggctgaatcc gagttgccgg cgtatgttcc
    11281 cggctttgct gccgtatgct cgcgaccagg ccatcggagc ggcggcaagt gaatgcgccg
    11341 aatcgcgaca cttcccaata agcacaacgc cgccatcgcc gcgagccaac ccgacagtgc
    11401 atcgtatgcg aaacgccgcg cgtcgcgatc attgaagatc ccggccaagg tgtcgcggac
    11461 ctttcccgag ctcagctcgc taatccgtcg cagcaatgcg cgatcggtgc cggtgggtcg
    11521 caactcgtcc ccgcgggaca acaccgcgcc ggtggtgccg aggctttgcg cagagtcgtc
    11581 cagcaggctc gccagataag aaccgggctg attcacgggt aaatgagcga tgtattggcc
    11641 tgccccggtg ggttcgaggt tgacctctcg cgtgtgacca tccgggcccg caactcgtgc
    11701 tgtcaactgc cgaaagctcg cgtgacgacc tt
//
LOCUS       DASTKU010000594         3479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1677914, whole genome shotgun sequence.
ACCESSION   DASTKU010000594 DASTKU010000000
VERSION     DASTKU010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677914"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VFQ61_20845"
     CDS             complement(<1..1111)
                     /locus_tag="VFQ61_20845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956963.1"
                     /translation="MLQLTALGAAAAGLGSYLKVQAEDQPLTTVDPRERKLLFVFCAY
                     GGASIIDSFLPIVESDVGDSQLSATLNVYPDSLVEQVSGSNLRHVGLLSDYLFYVKPT
                     NMGDFLRRHGQDVAVITHDVTSVNHAIGQQRSLNGAGFNAGRTLMEVMAMRYGGGMPL
                     PSCNMARNGFIEQGSDATVPLRARHELITSPMSFATGTHGYRGIAGLPASTHIDRARR
                     IREQLDRESVFARTFGKSPRLQTYLEQRKDAQEQFETADMIDKLLLLEPGKLDPKLGL
                     MPSDIVGALRNKLPVMAQNDTQAQLALGFLLAYHGISTSVTMGFREDPSIVDAGVLTT
                     PISFDFAHTSHRLTQSIMWGQTAALLDTFISLLKEY"
     gene            complement(1159..1881)
                     /locus_tag="VFQ61_20850"
     CDS             complement(1159..1881)
                     /locus_tag="VFQ61_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956964.1"
                     /translation="MSSANMRPRLLWSALLIACGSSGKPVPASSTSDSPPGAASPSSG
                     PRATDDVNIQSGDGAGTRTDAPSYQTSRRALVQWKRYAALEADLMSALKLSPEQVCTE
                     LGDQSCVRDVHLVSLGGNEPYVSGLMKPSSEPLATTPSVVDRVLLSACSARVRLDAAG
                     DKQVFMALDLHGPVPPRDDPSIASTIGDLYRKLLARDPEAREVELVAQLADDSAGERM
                     SAQDFAALACFTIGSTTEFLFF"
     gene            complement(1881..>3479)
                     /locus_tag="VFQ61_20855"
     CDS             complement(1881..>3479)
                     /locus_tag="VFQ61_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956965.1"
                     /translation="SVTQQGEMVRSNMVLRDMASFRIHRAWQGAFRRLRRPCGSIRLP
                     AWTHSVFVCILSYASLDPQSVAQARPEAPSIFCTKYPNAADCLGRFVECQECHESTFP
                     ASWNRFGTSLIGALPDDSDFAQGLPIALAAVENMDADGDGVSNLTEILSGTYPGDRSS
                     FWMKPPAQTAPENPRYRVDEYDFSFAFRRASILYCGRSPSYEELAVYRAPGADPSALR
                     NQLHERVDQCLNGPYWLKEGLKRLGDERIRPVKSAGPESKIIVYQWRLVIGDYEYDYR
                     LWNYVLSGGRDARDLLLAQYHIVDDSSGGVKRVEDPIPKPDEGAFAGGQPLQKEYRAG
                     MLTTQWYLARNTMFSELPRTSAAAAYRAYLGMDIAKTQGIFPVPGEPDDIDKKGVKEA
                     RCASCHSTLDPLAYAFAKYNGISRSGNPLFLLFGIFDFPFGDYDDGRPVQLIPGWSDS
                     EQQPVIMGQKVDSLRDWAEVAANSDEFARNVAQIFYVHALSRGPNAEEIDEFTALWRH
                     LPEDKYSANRLIHRLIDTHAFGVP"
BASE COUNT          638 a         1049 c         1163 g          629 t
ORIGIN      
        1 cgtactcttt gagtagcgag atgaacgtat cgagcagcgc cgcggtttga ccccacatga
       61 tgctctgcgt caggcgatgg ctagtgtggg cgaaatcgaa agagatcggg gtcgtgagca
      121 cgcccgcgtc cacgatcgaa gggtcctcgc ggaagcccat cgtcacgctc gtggagatcc
      181 cgtggtacgc gagcagaaag ccgagcgcga gctgcgcctg agtgtcgttt tgggccatga
      241 cgggaagctt gtttcgcagc gcgccgacga tgtcgctagg catcaagccc agcttgggat
      301 cgagcttacc cggctcgagc agcaagagct tgtcgatcat gtctgcggtc tcgaactgct
      361 cttgcgcatc cttgcgctgc tcgagatagg tctgcaggcg cggactcttg ccgaaggtgc
      421 gcgcgaacac cgactcccgg tcgagctgct cgcggatgcg ccgagcgcgg tcgatgtggg
      481 tcgacgcggg cagccccgcg atgccgcgat aaccgtgcgt tccggtggcg aaggacatgg
      541 gtgaagtgat gagctcgtga cgcgcgcgta gcgggacggt cgcgtcgctc ccctgctcga
      601 tgaacccgtt gcgggccata ttgcagctgg ggagcggcat gccgccgccg taccgcatgg
      661 ccatgacctc catcagcgtc cgccctgcgt tgaagccggc accgttcagc gagcgctgct
      721 ggccgatggc gtggttgacg gaggtcacgt cgtgcgtgat caccgcgacg tcctgtccgt
      781 gacgacgcag gaagtcgccc atgttcgtgg gcttcacgta gaacaggtaa tcgctgagca
      841 ggcccacgtg acggaggttg ctgccggaca cctgctcgac cagagagtcg ggatacacgt
      901 tgagcgtcgc gctgagctgc gagtctccca cgtcgctctc gacgatgggc aggaagctgt
      961 cgatgatgct cgccccgcca tacgcgcaga agacgaacag gagcttgcgt tcgcgcggat
     1021 cgaccgtggt caacggttgg tcctctgcct ggaccttgag gtaggaacct aggcctgctg
     1081 cggccgcccc gagggcagta agctggagca ttcgccgacg accgatttgg ggccgagagt
     1141 gagtcatgtt gctggacctc aaaagaagag aaattccgtc gtgctgccga tcgtaaaaca
     1201 ggccagggcg gcaaagtcct gagcggacat gcgttcgccg gcgctgtcgt ctgcgagctg
     1261 agcaacgagc tcgacctcgc gcgcctcggg gtcgcgcgcc aacagcttcc ggtagagatc
     1321 gccgatggtg ctcgcgatcg acggatcgtc tcgaggcgga accggtccgt gaagatcgag
     1381 cgccatgaac acctgtttgt ccccagccgc gtcgagccgc acgcgggcgc tgcaggcgct
     1441 gagcaacacg cggtcgacga ccgaaggcgt ggtggcgagt ggctcggagc tcggcttcat
     1501 cagaccagat acgtagggct cgttcccgcc caaagagacc aggtggacat cgcggacaca
     1561 gctctgatcc ccgagctcgg tgcacacctg ctccggggag agtttgagcg cgctcatgag
     1621 gtccgcttcc aatgccgcgt aacgcttcca ctgtactagc gcgcggcgcg aggtctgata
     1681 ggacggcgcg tctgttcgcg tgccagcccc atcgccggac tgaatgttta cgtcgtcggt
     1741 cgctcttggg ccgctgctcg ggctggcagc gccgggcggg ctgtctgaag tcgagctcgc
     1801 aggcacgggc ttaccactcg atccgcaggc gatgagcagc gcggaccaca agagccgggg
     1861 gcgcatgttc gccgacgaca ttagggcact ccgaaagcat gggtgtcgat caaacggtga
     1921 atcagccgat tggccgagta cttgtcctcg ggcaggtgcc gccacagcgc cgtaaactcg
     1981 tcgatctcct cggcgttcgg accgcgcgag agcgcatgca cgtagaagat ctgggccacg
     2041 ttgcgggcaa actcgtcgga gttggccgcg acctccgccc agtcgcggag actgtcgacc
     2101 ttctggccca tgatgacggg ttgctgctcg ctgtctgacc agccgggaat gagttgcacc
     2161 gggcgaccat catcgtagtc cccgaagggg aaatcgaaga tgccgaacag gagaaacagc
     2221 gggtttccag agcgcgagat gccgttgtac ttggcgaaag cgtaggcgag cggatccaaa
     2281 gtggagtggc agctcgcgca acgcgcttct ttgacccctt tcttgtcgat gtcgtccggc
     2341 tcgcccggca cagggaagat gccctgggtt ttggcgatat ccatgcccaa gtaggcgcgg
     2401 tacgcggcgg ccgcgctggt gcgcggcagc tcgctgaaca tggtgttgcg cgcgaggtac
     2461 cactgcgttg tcaacatccc cgcgcggtac tccttctgta gcggctgacc gccggcaaac
     2521 gcgccctcat ccggtttggg aatgggatct tccacgcgct tgacgccacc gctgctgtca
     2581 tccacgatat ggtattgggc gagcagcaga tcgcgggcgt cgcggccgcc gctcagcacg
     2641 tagttccaga gacgatagtc atactcgtag tcgccgatga cgagccgcca ctggtagacg
     2701 atgatcttgg actccgggcc tgcactcttc accggacgaa tgcgctcatc accgagccgc
     2761 ttcagcccct ctttgagcca gtaaggcccg ttcaagcact gatcgacgcg ctcgtggagc
     2821 tgattgcgga gcgcactcgg gtcggcgcct ggtgcgcgat agaccgcgag ctcttcgtag
     2881 ctcgggctcc ttccgcagta caggatcgac gctctacgga aggcaaagga gaaatcgtac
     2941 tcatccacgc ggtagcgagg gttttcgggc gcggtctgcg cgggtggctt catccagaac
     3001 gagctgcggt cgccgggata tgtgccgctc aagatctcag tcaggttcga gactccgtcg
     3061 ccgtctgcgt ccatattctc cacggcggcg agcgcgatcg ggagcccttg cgcaaaatcc
     3121 gaatcgtcgg gcaacgcgcc gatgagagaa gtgccgaagc gattccacga ggcggggaaa
     3181 gtggactcgt gacactcttg gcattccacg aagcggccca agcagtctgc cgcgttggga
     3241 tacttcgtac agaagatgga gggggcttcc ggccgagcct gcgccacgct ctgcggatcc
     3301 agactggcgt atgagagaat gcaaacaaag acagagtgcg tccaagccgg aagccggatc
     3361 gacccgcagg gcctccgcaa gcggcggaag gcgccctgcc atgcacggtg gatgcggaaa
     3421 gaggccatgt cgcgcagtac catgttgctg cggaccattt cgccttgttg ggtgacaga
//
LOCUS       DASTKU010000595         3993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_561767, whole genome shotgun sequence.
ACCESSION   DASTKU010000595 DASTKU010000000
VERSION     DASTKU010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561767"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..538
                     /locus_tag="VFQ61_20860"
     CDS             188..538
                     /locus_tag="VFQ61_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956966.1"
                     /translation="MDIHTLYSSALCALFVFWNGVRIFTYVPTILKLLRPEAEARDYS
                     LTTWGSWVCSNGFFALYLWEQSHRQLNAMVLLNTGNTLMCAITCCCIFRLQHRTARQP
                     DSNHVPAVRTLHRV"
     gene            673..1998
                     /locus_tag="VFQ61_20865"
     CDS             673..1998
                     /locus_tag="VFQ61_20865"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9956967.1"
                     /translation="MNTQHVLSSSSKSSTLGPRSALRRILGALAPALMLVGASSAFEP
                     QAAAQAASAQAAPAPALSMELWSPEYGYNDGWRVDANPRLIGDVNGDMLDDVVGFGPM
                     GVEVSLSTGDSFETPKFWVLDYTVAQGWSSAKHVRVLADVNGDGRKDIVAFGNGGTYV
                     SLSTGKSFYPAQLWIADYGYDGSWRLDLHVRLLADVNGDMMDDIVAFGTGGTYVSLST
                     GAVFRPPQLWVKDYGYNAGGWRVEQHERRLADVNGDGLKDVVGFGTGGTYVSLNTGKS
                     FLPPKVWVSEYGYDAGGWRVDKHPRFLADVNGDMKDDVVGFGPNGTYVSLSTGSFFTP
                     AEIWIADFGFDVGGWRLDKHPRFLADMDGDGMSDVVGIGYGGVVISKSTGHSFASPTL
                     AIANYGFDAGGWRVDQHPRLLADVDGDNTPDVVGFGGAGTYVTLTNPYH"
     gene            complement(2105..2569)
                     /locus_tag="VFQ61_20870"
     CDS             complement(2105..2569)
                     /locus_tag="VFQ61_20870"
                     /inference="COORDINATES: protein motif:HMM:NF020254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HET9956968.1"
                     /translation="MLEPFEAPAAIQQALLILDSYARVLGKPLLEAHGTPTERARALY
                     RAPFVVLSHGTAVDPVFNYGNLQAQQLFEFDWESLTQLPSRLSAEPVNQAERQRLLES
                     VSSRGYIDDYAGIRISRTGRRFRIERATVWNLMDEAGHRQGQAATFADWTPV"
     gene            complement(2563..3579)
                     /locus_tag="VFQ61_20875"
     CDS             complement(2563..3579)
                     /locus_tag="VFQ61_20875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HET9956969.1"
                     /translation="MAELSHYVNTLGRALRERYGERVHKVAIDAGFTCPNRDGSAGRG
                     GCTFCNNASFSPNHVGAEAIETQIDRGRAVIARRTGARRLLAYFQAYTNTYAAVSTLS
                     ELYDRALNQPGVIGLSVGTRPDCVPDAVLDLLESYADRGLEVWLELGLQSSFDETLRR
                     VNRGHGFPEYRDAIRRARHRGLQVCTHLITGLPGETAEHALTTLARVLDEGTQGLKLH
                     PLHVVRGTLLARDLRLGTYQPWPLQDYVDVAVEMIERTPWPIVYHRLTGTAPAPVLLG
                     PAWCSSKWRVLNAIRGELERRGTRQGHATSEPAFGLAPASNSGVDAQSSCSEQADRAN
                     ACPC"
BASE COUNT          764 a         1193 c         1249 g          787 t
ORIGIN      
        1 agggtcggca agaccgaagc gagatccgct tcggattggc tgacggcgcg cgagcacgtc
       61 cgcacccgag aggaggcgga gagcgcgagc agtggccggg ccctagacga ggacgcgcga
      121 tatcaggcct agctgcgaat gagcgcagcg cagattacgt ggcggcgagc tgcccaaagg
      181 ttttcgaatg gacattcaca cgctctactc gagcgcgctc tgcgctctgt tcgtcttctg
      241 gaatggcgtg agaatcttca cctatgttcc tactatcctc aagttgctcc ggcccgaagc
      301 cgaagcccga gactacagcc tgaccacttg gggatcgtgg gtctgctcca atggcttctt
      361 cgcactgtat ctgtgggagc agagccatcg acaactcaac gcgatggtgc tgctcaatac
      421 cggaaacacc ctgatgtgcg cgattacgtg ttgctgcatc ttcaggctgc aacaccgcac
      481 ggcgcggcaa ccggactcga atcacgttcc cgccgtacga acgctacatc gggtgtgatc
      541 cgcccgatgc tcgactgagc gccgcgacct cccggatcac tcaacctctt gcaacgcgca
      601 ttcgtgcgcg cttgcgagcc tgcgacccaa gttgaaccca aaacccaatc aaaacagaaa
      661 gagacaccca ccatgaacac tcaacacgtc ctcagctcct cctccaagtc ttccaccctt
      721 ggcccccgtt cggcgctgcg cagaatactt ggagctttgg cccccgccct gatgttggtc
      781 ggcgcttcca gcgcgttcga accgcaggcg gccgcgcaag ctgcttccgc ccaagccgca
      841 ccggcacccg ccttgagcat ggagctctgg agcccggaat acggctataa cgacggctgg
      901 cgagtcgacg ccaatccaag actcataggg gatgtaaatg gggacatgct cgacgacgtg
      961 gtcgggttcg gcccgatggg ggtcgaggtt tcgctttcca ccggcgattc gttcgagaca
     1021 cccaagtttt gggtcttgga ctacaccgtg gcgcagggct ggagttcggc caagcatgtt
     1081 agggtgctcg cagacgtgaa cggcgacgga agaaaggata ttgtcgcctt tggaaacggt
     1141 ggaacctacg tctcactctc gacgggcaag tcgttctatc cggctcaact ttggattgca
     1201 gactacggat acgacggcag ctggcgcttg gacctgcatg tacggcttct cgccgacgtc
     1261 aatggagaca tgatggatga tattgtcgcg ttcggcactg gtggaaccta tgtttccctc
     1321 tcgaccggcg ccgtcttccg tccgccacag ctttgggtca aggactatgg atacaatgcc
     1381 ggcggttggc gcgtggagca gcatgaacgt cgcctggctg acgtgaacgg tgacggactc
     1441 aaagacgtag tgggcttcgg cacgggtgga acgtacgtgt cactcaacac gggcaagtcc
     1501 tttcttccac cgaaagtttg ggtttccgag tatgggtatg atgctggtgg ttggcgcgta
     1561 gacaagcatc cacgttttct ggctgacgtg aatggcgaca tgaaggatga cgtcgtgggc
     1621 tttgggccga atggaactta cgtttcgctg tctaccgggt cattctttac ccccgccgag
     1681 atctggatcg ccgactttgg attcgatgtc ggtggctgga gactagacaa gcatcctcga
     1741 ttcctggctg atatggatgg tgacgggatg agtgacgtgg tcggcatcgg ctacggaggt
     1801 gtcgtgatat cgaagtcaac gggacactcg ttcgcgtcac ccactctcgc aatcgcgaac
     1861 tacggattcg acgcaggcgg ctggcgcgtc gatcagcacc ctcggttgct cgcagatgtc
     1921 gacggagaca ataccccgga tgtggtagga tttggaggcg ccggcaccta cgtcacgctt
     1981 accaacccgt atcattgaga ctgacggcat atcggatcga gctccggcgg gccgcccccc
     2041 cccggagagc aacaagcccg cgactgactc gttcattcgc gggcttcgac ctaaacggct
     2101 gcgctcagac tggcgtccag tcggcgaagg ttgcggcttg gccttggcgg tggcccgctt
     2161 cgtccatcaa gttccaaact gtcgcccgtt cgatacgaaa acgtcggccc gtacggctga
     2221 tccgaattcc cgcgtaatca tcgatatacc ctcgcgacga taccgattcg agcaggcgtt
     2281 gtcgctcggc ttgattgacg ggctccgccg agagacgcga ggggagctgg gtgaggctct
     2341 cccaatcgaa ctcgaacagc tgctgtgcct gcagattgcc gtagttgaac acgggatcga
     2401 ccgcggttcc gtgcgatagc acgacaaaag gcgctcgata caacgctcgg gcccgctccg
     2461 tgggagtgcc gtgggcttcg agcagaggtt tgcccaaaac ccgggcgtag ctatccagaa
     2521 tgagcagagc ctgctgaatg gccgccggag cttcgaaggg ctctaacatg ggcaggcgtt
     2581 tgctcgatct gcctgctcgc tgcaactgct ttgagcgtcc acccccgagt tcgatgccgg
     2641 ggctagtccg aaagcaggtt cactcgtagc gtggccttgt cgagtgcccc gccgttcgag
     2701 ttcaccgcgg attgcgttga gaacacgcca cttggagctg caccatgccg gccccaacaa
     2761 gacgggagcc ggagccgtgc cggtcaaccg gtgatagacg attggccacg gcgtccgctc
     2821 gatcatctcg accgcgacgt cgacatagtc ttgcagcggc cacggctgat aggttccgag
     2881 gcgcaagtcg cgagccaaca gcgttccgcg caccacgtgc aagggatgga gtttgaggcc
     2941 ttgggttccc tcgtcgagca cgcgcgccag cgtggtcagg gcgtgctccg cggtctcccc
     3001 gggtaagccg gtgatgaggt gcgtgcagac ctgcagtcct cgatggcggg ccctgcgaat
     3061 ggcatcgcga tactccggga agccgtgtcc gcgattcacg cgccgcagcg tctcgtcgaa
     3121 ggatgactgc agacccagct cgagccaaac ctccaaccca cgatccgcgt aagactccag
     3181 caagtcgagg acggcgtcgg ggacacagtc cggccgagtg cccaccgaga gaccaatcac
     3241 tccgggttgg ttgagcgcac gatcgtatag ttcggagagg gtcgagacgg cagcgtaggt
     3301 attcgtataa gcctgaaaat atgccagcaa tcgacgtgcg ccggtgcggc gggcaatcac
     3361 cgcgcgcccg cggtcgattt gcgtctcgat cgcctcggcg cccacgtgat tggggctgaa
     3421 agaggcgttg ttgcagaagg tacaaccgcc gcgaccggca gaaccatccc gattggggca
     3481 ggtgaatcct gcgtcgatcg cgaccttgtg cactctttcg ccgtagcgtt cgcgcaacgc
     3541 gcggccaagt gtgttcacgt agtgggagag ctctgccacg gatcgggcct acattcgagc
     3601 tcgccaagcg tcgggtccgg gttgcttgac cgctcgcgcc tactctagtg gctagtcgcg
     3661 gcccgtctcg tagagaattg atggctgccg agttcggcct gctcgacatc atggcactga
     3721 gctaggagcg gcaggccggt gccgtacgtt cgatctgccg ccctactggg cagcgtccct
     3781 gcactgggtc actaccgagg cacactgccg agggaccttg tctgatctgc cctcgaacca
     3841 ttgcgccgcg agactagtgc accgcatgtg agatgcgatc aagcattcgg cggtggctgc
     3901 ggatgatagc ggcagcgggc ttggtccaga tgaagggcgt cgggtccttg ttccacccgg
     3961 cgatgtagga agcgatgtgg tctcggagtt gct
//
LOCUS       DASTKU010000596         8479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1902659, whole genome shotgun sequence.
ACCESSION   DASTKU010000596 DASTKU010000000
VERSION     DASTKU010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902659"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VFQ61_20880"
     CDS             complement(<1..484)
                     /locus_tag="VFQ61_20880"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956970.1"
                     /translation="MPTLSSNIVKRDIASVHDVEKALARQALYGGDLPTNLLEVAAVS
                     EADLTRIIAETSGLQAAPVGELPRAEDAVLSTIASSVAQRYGLYPLSKQGNVLNVAVS
                     DPLLPAIVAELEFSHGVKLVQQATTRVRVEQAIARDYGLELNRRVARVLAKVEGDRDP
                     Y"
     gene            complement(666..1688)
                     /gene="recA"
                     /locus_tag="VFQ61_20885"
     CDS             complement(666..1688)
                     /gene="recA"
                     /locus_tag="VFQ61_20885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET9956971.1"
                     /translation="MSSDKKKPDLLRDVLATVEKACGQGAIMRLGEAAFEPIPAFPTG
                     AMALDTALGVGGYPRGRIIEIFGPESSGKTTLALHAIAEVQTLGGIAAFIDAEHAFDL
                     RYAKSVGIDFSRLLVSQPDCGEQALEIAELLTRSGSVDLIVIDSVAALVPKAEIEGDM
                     GDAHMGLQARLMSQALRKLTGVANKTQTTLIFINQLRQKLGVVFGSPETTTGGNALKF
                     YASVRLDVRRTGKLSVGEAVVGNRTRVKVVKNKVAPPFTEAEFDIRWGSGIDVAGDLL
                     EMGIARGVVDKNGSHLSFQGKPLGHGRDRAREQLLSSPELKAELRSAVLEQGRHPQTI
                     DATAAA"
     gene            complement(2326..2775)
                     /locus_tag="VFQ61_20890"
     CDS             complement(2326..2775)
                     /locus_tag="VFQ61_20890"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9956972.1"
                     /translation="MFKNRKILVPVDFSSCSKTALEHALALAALTGAQIDLLYVWEPP
                     PFEGREPRVHVNGEASTLQDYALTQSRTEMDKFIAGVPEEQRKRLQAHYEVGRPRERI
                     LDHATRGYDLVVMGTHGRTGRARALAGSVAESIVRGCVSPVLTVREH"
     gene            complement(3119..4363)
                     /gene="dinB"
                     /locus_tag="VFQ61_20895"
     CDS             complement(3119..4363)
                     /gene="dinB"
                     /locus_tag="VFQ61_20895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET9956973.1"
                     /translation="MSAERWILHADMDAFYASIEQRDHPELANQPVIVGAGSARGVVA
                     AASYEARKFGVRSAMPGFQARKLCPQAVFLPGNMEHYAAVSAEVHEVFLRFTPEIEPL
                     ALDEAFLDITGSRALFGAPEQIGRKLREAVFESTRLPVSVGIAPNKLVAKLACTLAKP
                     NGMKIVPPEEITSFLAPLPVRRLWGVGPVLATQLTEAGFMTFADLWHAPEDELRAIVG
                     RRAPALRALARGEDARPVQPDRAPKSYGEENTFEHDVSAVEIITAALTAHAEAVARRV
                     RTDGIRGRTVTLKIKLGRARETRTSRIDHNVSEPVYPLLTRSRTLPHATDSGAELRKI
                     AIRLWHDADIREPVRLLGVSLSNLESLSGLQLELFDRPREDKLGPVMDAIHERFGKAA
                     ITRAVQAPGKLTASSRRKHGDK"
     gene            complement(4373..4876)
                     /locus_tag="VFQ61_20900"
     CDS             complement(4373..4876)
                     /locus_tag="VFQ61_20900"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET9956974.1"
                     /translation="MSANSKQTVVEEGTEFKGTLVSSCPIVVRGRIEGNVDTPALSVS
                     EKGAVHGRAKVGSLRSLGELSGEFDAETVELSGKVKDSTVIRAKTLEVKLASEDEKMQ
                     LTFGNCELAVGDAPQDDASDARRSRAPKASKAPPLDITASSTSSASPTPPPNLGSNPG
                     AGDVSLP"
     gene            complement(4873..6117)
                     /locus_tag="VFQ61_20905"
     CDS             complement(4873..6117)
                     /locus_tag="VFQ61_20905"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET9956975.1"
                     /translation="MAELTRDEVLKRAGAKESLSREDLRGLDLSDALLEGIDFSRAEL
                     DGANLQGAKLRGANFRNASLREAFLKQADLREANLDKADLEGANLERCDLSEANLNRA
                     NLEGANLTEAVLRLARLNGAQLDSAELGKANLERAVLAHAELVECYFGGANLKFADLS
                     NAVLTSANLEEAVLERAKLEEAELGQISAPKANFSGALLSKTNLSKARLSGAKLDSAD
                     LRHANLTGSDLSNASLTGAKLFGAVTSNAKWVGYAAQWLDASPNGDGSKKATGDDIEI
                     LLSGRLPTPGRAARYFGRGDVLRDALLEFGDGSSIEIESRFENCAILLGAGTDLIVGE
                     AAVLSQCRISGGGNITIHGKFFESESPGIRGPKTLVVSTTGSLVSTIEQSTEATRFAF
                     EPGCKLRIKILKPQDAERSGME"
     gene            complement(6211..7761)
                     /locus_tag="VFQ61_20910"
     CDS             complement(6211..7761)
                     /locus_tag="VFQ61_20910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET9956976.1"
                     /translation="MTHAGLGPVTAVIPASSGSSGAGSIAEALADASERAKSTESSLG
                     TEKNPALATALGPEQKSSLYWLSPQGAATDATATPIPGSPVSLNKARDAAGTESPVAE
                     APVGVEAEAPVEVEMELEPENPPALPPPLFRRRSEPPSAAPHSAPVGSGVVSSSAEVS
                     SSTVARFAASEVVAATDAVSPPNTLSDIDERFESIVSRPPAAPEPPSATADLSATAES
                     SAAIAEPPMLPVVTDAAPTLSSNADLEDARRLFVELSGAYLGPLRDLMLEIELTDPSR
                     EWLAVCRPAVASLSQAAKELGMESLVRALEGMRSALDDAGRGLGSIIDLRGREALRTA
                     YRSLVAELPGAFSVTQERDRREPLIVQSLLLQVPEVRKVALDRIYSAGLCALEHFYKA
                     KPAEIAETAGIPRELAERIVARFQRYRRETGSMPPSARKGRERTQLENLARKLEEQNS
                     AYDASSQSFGSGEDKRRLRQERAATLLEVNLLLARSGELRLIEKLERSTFQGKAEALR
                     KYLSERAE"
BASE COUNT         1442 a         2737 c         2627 g         1673 t
ORIGIN      
        1 gataagggtc gcgatctccc tcgactttgg cgagcacgcg ggccacccgc cgattcaatt
       61 cgagcccgta gtcgcgcgcg attgcctgct ccacgcgcac gcgagtcgtg gcctgctgca
      121 cgagcttgac gccgtgcgag aactccagct ccgcgacgat ggccgggagc aacggatcgc
      181 tcaccgcgac attgagcacg ttaccctgtt tgctcagagg atataatccg tagcgctgcg
      241 cgacgctgct cgcgatcgtg ctcaataccg cgtcttccgc gcggggcagt tcaccgaccg
      301 gtgccgcttg cagccccgag gtctccgcga tgatgcgcgt gaggtccgcc tcactgaccg
      361 ccgcgacctc gagtaggttc gtgggcaggt caccgccgta gagggcttga cgagccaagg
      421 ctttttccac gtcgtgaaca gaggcgatgt ctcgtttgac gatgttggag ctaagcgtcg
      481 gcataggcgg cagccgaagg cagataccct tcgttggcga atgttcacgg ggcttcagcg
      541 cctgtcaaca catgagcgcg ctttattcgc tcgatcgacg ggccggctgc gtgccggttg
      601 gaaccagtcg tgtttgctcc gatacgcaca ccccacgact ggttcctgca cgtcgtggcg
      661 cccgcttagg ccgcagccgt agcgtctatg gtttgcgggt gccgcccctg ctcgagcacg
      721 gccgagcgga gctcggcctt gagctccggt gagctcaaca gctgctcccg agcacggtca
      781 cgtccatggc ccaggggctt accctgaaac gagaggtggg acccgttctt gtccacgact
      841 ccgcgcgcga tgcccatttc caggaggtcg cccgcaacgt cgattccact accccatcgg
      901 atatcgaact cggcttccgt gaacggcggc gccactttgt ttttcaccac tttgacgcgg
      961 gttcggttac ctacgactgc ctcgccgacg ctaagcttgc ccgtacgccg gacgtccagg
     1021 cgcacgctcg cgtagaactt gagggcgttc cctccagtgg tggtttcggg gctaccgaat
     1081 acgaccccca gcttttgccg cagctggttg atgaagatca gcgtggtttg ggtcttgttg
     1141 gcgacaccgg tcagctttcg gagggcctgg ctcatcaagc gtgcctgaag gcccatgtgt
     1201 gcgtcgccca tgtcgccctc gatctcggct ttcggcacta gcgccgcgac cgagtcgatc
     1261 acaatcaggt ccacgctgcc tgaacgcgtc aggagctccg cgatttccag cgcctgctct
     1321 ccacaatcag gctgcgatac gaggagtcga ctgaagtcga tgcccacgga tttcgcgtat
     1381 cgaaggtcga aggcatgctc cgcgtcgatg aacgccgcga tcccgccgag cgtctgcacc
     1441 tcggcgatgg cgtggagcgc gagagtggtc ttgccgctcg attcggggcc gaaaatctcg
     1501 atgatgcgtc cgcgcggata tcctccgaca ccgagcgccg tgtccagcgc catcgcgccg
     1561 gtgggaaacg cgggaatagg ctcgaatgca gcctccccca agcgcatgat ggcaccctgc
     1621 ccgcatgcct tctccacggt cgccagaacg tctcgcagca gatctggctt cttcttgtct
     1681 gagctcacga cagattcctt cctgagccgc tcgtgcggct cgggatcgga atcagcagct
     1741 cacatgccgt gcgaaaatcc cgtgaatttg cgcaaagtgg gcctggtcgc tgctggccat
     1801 gttctggcga gtctgccgat tcgccaggta cagctggtcg ccttgggatc cgttgccttt
     1861 ttggagtcac cgggtcgggt acgcattgcg accgcgaact ccaacaggag cttggtgcag
     1921 cgctcgtggc cgattgaggt tttcgatacc gtcgagcttg ccgacgcgca ctctctccgc
     1981 tcaaaccttg gctgctcgcc gtcgcactgc gtcagccgcc aagaacggaa ggagtaggac
     2041 accgccgggc gcgaccacga cggccgtcca gagtaaggct cgaatcacac cgcgcatctg
     2101 tccagcacct tcgagcccca cacatgcaag aagcgtgcgt ggactcaatg ggcaagtttt
     2161 cggctgattt tgccgaaaac tagatccgcc aaacagccgt catcacaatc cgcccggatc
     2221 tttgtgtcat agcgacagca cggacgtcat gcgcccgggg ctgtgacacg cgttcaccaa
     2281 cctcgaacaa gccggcgggc gctcatctcg acgggcgacc cacggtcagt gttcgcggac
     2341 agtgagcacc gggctgacgc agccacgtac gatgctctcg gcgacactac cggccaaagc
     2401 gcgagcacga cccgtgcggc cgtgggtgcc catcactacg aggtcatagc cgcgtgtcgc
     2461 atgatccaag atccgctcac ggggacgacc tacttcgtag tgcgcttgca agcgcttgcg
     2521 ctgctcttca ggcacacccg cgatgaactt atccatctcg gtgcggcttt gcgtgagtgc
     2581 gtaatcctgc agcgtcgagg cctcaccgtt cacgtggacg cgcggttcac gaccctcgaa
     2641 aggcggtggc tcccagacat acaaaaggtc gatttgtgca ccagtgagag cggccaaagc
     2701 gagcgcgtgc tctagcgccg tcttggagca agaggagaag tccacgggca cgaggatctt
     2761 gcgattcttg aacataacgt tactccgagt ggggcttagt ccgccaacgt atcgacgtcg
     2821 cgagtcaagc tcccacgcgc cgagtctgca acggacatgc ctcgagagca acggcccgga
     2881 aaggccagtt aattacctcg atcagatcga catacacgca aactctgcgt cgtggcgcga
     2941 gcagcctgcg cccaagctcg atatcgctcc aagcgtgcgc atgactgctc gacctccgca
     3001 cgcggtcctg ccatctggct cgagctgcgc tcgcggagcg gcgagaaacg agcgtcgctt
     3061 cgcactcggg cgccgcctcg gggcgctcct actgggatgg gcccaagcac acgagggttt
     3121 acttatcccc gtgtttcctc cggctagatg cggtcagctt acctggggcc tgtaccgcgc
     3181 gcgtgatggc tgcttttccg aaccgttcgt gaatcgcatc cattacgggg ccaagcttgt
     3241 cctcgcgcgg gcgatcgaag agctcgagct gcaggccact caagctctcc agattggata
     3301 gtgacacacc caacaaccga accggttccc ggatatcggc gtcatgccac aacctgatcg
     3361 cgatcttgcg cagctcggcg ccactgtccg tggcgtgcgg cagagttcgg ctgcgggtga
     3421 gcagcgggta aaccggttcc gagacgttgt gatcgatgcg cgaagtgcgg gtctcgcgtg
     3481 cacgtccgag tttgatcttt agagtgaccg tgcgtcctct gatcccgtcc gtgcgaacgc
     3541 gccgagccac ggcttcggcg tgggcggtca aggccgcggt gatgatctcg accgcgctca
     3601 cgtcgtgttc gaacgtgttt tcttcgccgt aagacttggg ggctcgatcg ggctgaacgg
     3661 gccgagcatc ttcacctcgg gcgagcgctc gaagcgcggg cgcccggcgg cccacgatcg
     3721 cccgcagctc gtcttctggc gcatgccata ggtcggcgaa cgtcataaac cccgcttcgg
     3781 tgagctgggt tgccaacact ggtccaacgc cccaaaggcg ccgaaccggt agcggcgcga
     3841 ggaacgaggt aatctcttcg ggcggcacga tcttcatgcc gttgggcttc gcgagcgtgc
     3901 atgccaactt cgcgacgagt ttgttcgggg cgatgccgac cgagacaggc agacgtgtgc
     3961 tctcgaacac tgcttcgcgg agcttgcgtc cgatctgctc gggggcgccg aagagcgcgc
     4021 gcgagcccgt gatgtccaag aacgcttcgt ccaaagcgag aggctcgatc tccggcgtga
     4081 agcgcaagaa cacctcgtgc acttcggcgg agaccgccgc gtagtgttcc atgttgccgg
     4141 gcaagaacac ggcttggggg cacagtttgc gtgcctgaaa gcccggcatt gcggaacgca
     4201 cgccgaactt gcgcgcctca taggaggcgg ccgccaccac tcctcgcgcc gaacccgcgc
     4261 cgacaatcac gggctgattg gctagctctg gatggtctct ctgctcgatg gacgcgtaga
     4321 aggcgtccat atcggcgtgt agaatccagc gctccgcgct caaaggcgcg ccttacggca
     4381 gcgaaacgtc gcccgcgcct ggattcgagc ctaggttcgg gggcggcgtc gggcttgcgg
     4441 atgaggttga gctggcggtt atatcgagtg ggggggcctt ggaggctttg ggcgcgcggc
     4501 tgcggcgagc atcgctggcg tcatcctgcg gagcatcgcc tacggcgagc tcgcagttgc
     4561 cgaacgtgag ttgcatcttc tcgtcttcgg aggcgagctt cacttctagc gtcttggcgc
     4621 gaatgacggt gctgtctttc actttgcccg agagctcgac cgtttccgcg tcgaattcgc
     4681 ccgagagttc accgagcgac cgcaacgagc ccaccttggc acgtccgtgt accgcgccct
     4741 tttcgctcac ggaaagggcc ggcgtgtcga cgttcccttc gatgcgtccg cgcaccacga
     4801 tagggcacga cgagaccaaa gtgcctttga attcggtgcc ctcctcgacg accgtctgct
     4861 tcgaattcgc gctcattcca tacctgacct ttccgcatcc tgcggcttca aaatcttgat
     4921 tcgcagcttg caacccggtt cgaatgcgaa gcgcgtggct tcggtcgact gctcgatcgt
     4981 cgacaccaac gaacccgtgg tgctcacgac gagtgttttg ggtccacgaa tcccagggct
     5041 ctcgctctcg aagaacttac cgtgaatggt gatgttaccg ccgccactga tgcggcactg
     5101 actcaacacg gccgcttccc cgacaatcag gtcggtgccc gcgccgagca ggatcgcgca
     5161 gttttcgaag cggctctcga tctcgatgct cgatccatcg ccgaactcga gcagtgcgtc
     5221 ccgcaatacg tctccacggc caaaatagcg ggcggctcgt cctggggtgg gcaagcggcc
     5281 cgacaggaga atctcgatat cgtcgccggt cgcctttttc gacccgtcac cattcgggct
     5341 ggcatccagc cattgcgcgg cgtagccgac ccacttggcg ttcgaggtga cggcaccaaa
     5401 gagtttggcg cccgtgagcg aagcgttcga caggtccgag ccagtcaagt tggcgtgacg
     5461 caagtccgcg ctgtctagct tggcgccgct caaccgcgct ttgctcaggt tcgttttcga
     5521 gagcagcgcg ccgctaaagt tggcttttgg cgcggagatc tggccgagct ctgcctcttc
     5581 cagcttggct ctttcgagca ctgcttcctc caaattggcg gaggtgagta cggcgttgct
     5641 tagatccgca aacttgagat tcgctccccc gaagtagcac tcgaccagct cggcgtgggc
     5701 caacacagcg cgttccaaat tggccttacc cagttcggct gagtccagct gcgcgccatt
     5761 caagcgcgcc aggcgcaaca ccgcttccgt cagattggcg ccttccaggt tcgcgcgatt
     5821 caagtttgct tcgctcaagt cgcagcgttc caagttggcc ccctcgagat ccgctttgtc
     5881 gaggttggcc tcgcgcaaat cggcctgctt caggaaggct tcgcgcaagc tcgcgtttcg
     5941 gaaattggcg cctcgcaact tggcgccttg caggttcgcg ccgtcgagct ctgcgcggct
     6001 gaagtcgatc ccttcgagga gtgcatccga caggtctagt ccgcgcaggt cttcgcgcga
     6061 caggctctct ttggcgcccg cgcgcttcag cacttcgtct cgagtaagct cagccataat
     6121 tcaaggatcc tccgttcgtt gctcgcggtc gggctgctac gtcactctgt ttcccagtca
     6181 ctctgcttgt tcggtcactc tgcctgtcag tcactctgct cgttcggaaa ggtattttct
     6241 aagcgcttcg gccttacctt gaaacgtcga acgttcgagc ttttcgatca gccgcagctc
     6301 gccgctccga gcaagcaaca ggttgacttc caggagagtg gccgcgcgtt cctggcgcag
     6361 gcgccgtttg tcttctccgc tgccgaacga ctgcgagctc gcgtcgtaag cggagttttg
     6421 ctcctctaac ttgcgtgcga ggttttccag ctgcgtgcgc tcacggccct ttcgcgcgct
     6481 cggcggcatc gagcctgtct cgcgccgata gcgttggaac cgagccacga tgcgttcggc
     6541 gagctcacgc ggaatgcccg ccgtctcggc gatctcggcg ggcttggcct tgtagaagtg
     6601 ttcgagagcg cacaatccgg cggagtagat gcggtccaac gcgaccttgc gtacctcggg
     6661 gacctgcagc agtaacgact gcacgatcag cggctcacgg cggtcccgct cttgcgtcac
     6721 cgagaacgcc ccgggcagct ccgcgaccaa gctacggtac gctgttctca gcgcctcgcg
     6781 gccacgcaga tcgatgatgc tgcccaagcc tcggccggcg tcatcgagag cactacgcat
     6841 gccctccaaa gcccggacca gggactccat gccgagctct ttggcggctt ggctcagcga
     6901 ggcaacggcc gggcggcaaa ccgccagcca ctcgcggctc ggatcagtga gctcgatctc
     6961 cagcatcaga tctctcaggg gcccgagata agcgcccgag agctccacga acaaacgtcg
     7021 agcgtcttcc aaatccgcgt tcgaagacag cgtcggcgct gcatccgtca cgacggggag
     7081 catcggcggc tccgcaattg cggcagatga ttcggcggtg gcgctgagat cagccgtcgc
     7141 agacgggggc tcgggggcgg caggcggccg cgatacgatg ctctcgaacc gttcgtcgat
     7201 gtccgagagg gtgttcggtg gagacaccgc atccgtagcc gctacgacct ccgaagccgc
     7261 gaaacgcgcg acagtgctac tcgacacctc ggcgctactc gagacgacgc cggacccaac
     7321 cggcgcggag tggggggccg cagacggcgg ttctgaccgc cgcctgaaca aaggcggggg
     7381 aagcgctgga ggattctcgg gctccagctc catttctacc tcgacgggag cttccgcttc
     7441 taccccgacg ggagcttccg cgacggggct ctctgttccg gcggcgtccc gcgccttgtt
     7501 caagcttaca ggtgatccag gaatgggcgt cgcggttgcg tcagtggcgg ccccctgcgg
     7561 gcttaaccag tagagtgacg acttctgttc cgggcccaac gccgttgcca acgcagggtt
     7621 cttctctgtg cccaggctgc tttccgtcga tttcgcgcgt tcactggcat ccgccagcgc
     7681 ctccgcgatc gagcccgccc ccgacgaacc agaggacgcc gggatcaccg ccgtcactgg
     7741 tccaaggccc gcgtgtgtca ttgccaggtg cgtcggcccc gcttggctaa ggccaggttg
     7801 ggttgggccg acttgtgtag gccctgcttg cgtgggtccc ggcagcgtga gccccgcctg
     7861 agttggagac gcgccggagc tgggggcagg gggtggtccg ctgcgcccca gggctggaat
     7921 cgtacgacgc ggcggaagtg gacgtcgggc caacggcggc aactgaccgg gcgatgttgc
     7981 cttgggctgg ctcccaaccg caggtgattt gcttggcgtt tggggcacgc ccgccggggg
     8041 tggtggtgcc gtagctacag gtttggagtc tgcctgtggc aaacgcccgc cgcactccac
     8101 gcagaacaga tctccatccg agctgagtgc gccgcagtgg ggacatcgag ccgtaagacc
     8161 cgccatggcg aacgggagac ttagcatcca tggagcgctt tcgggcgact aattcgaaag
     8221 agtttcctcg cccacattgg agcacccatt ggctagtttt cgctcgcggc gccacacgcg
     8281 ctcgcgccgc cgtcgcttac atatctccag gccatgtact ccccaggcca tgtactcccc
     8341 aggccatgga ttctcctgcc atgcatcccg ggtcgtgtat ctctcgcggg tgctatcggc
     8401 atgcagggtt tgtctcaagc gtcggtgaat aggtttgcgg tccgttccgc agaccgtatc
     8461 gccgcgtcga tgcccgagc
//
LOCUS       DASTKU010000597         4581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_562364, whole genome shotgun sequence.
ACCESSION   DASTKU010000597 DASTKU010000000
VERSION     DASTKU010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562364"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(736..1107)
                     /locus_tag="VFQ61_20915"
     CDS             complement(736..1107)
                     /locus_tag="VFQ61_20915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956977.1"
                     /translation="MAKLPQDFVRPSPTNSERVFAALEAFGAPLRAHSVAPGHFAREG
                     DAYRFGIAPLKIEVLTKISGVSFDEALRGSGTFELEGHAIPYIGKAALIANKISASKT
                     LQMSKSSSASASAELRRAMTR"
     gene            complement(1100..1351)
                     /locus_tag="VFQ61_20920"
     CDS             complement(1100..1351)
                     /locus_tag="VFQ61_20920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956978.1"
                     /translation="MATEVEALEARKLRAAARRARMTVEVVALGERKLPPYADSTPEE
                     RLAAAVRLIEHHQALRGGFSKVPRDAWPGETFVIGERVG"
     gene            1405..1824
                     /locus_tag="VFQ61_20925"
     CDS             1405..1824
                     /locus_tag="VFQ61_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956979.1"
                     /translation="MPRQNSWYIGGDPLEMLDPFGRSPGARSRNGALSLSRAVSGCGR
                     YSSSQAKKAVTCGRKFKIIEQDMVALGELDEKVPFLAGLPSSVIRNRLHAHPGRIRRD
                     AEGGGQSVLSHGLYDGARTAVTDCLLPARQRTAIGRR"
     gene            1834..2733
                     /locus_tag="VFQ61_20930"
     CDS             1834..2733
                     /locus_tag="VFQ61_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956980.1"
                     /translation="MTRRSLATLGLAVLLATAGGLLLFRKAAPVDPSALRVRATALSV
                     KHRIRVEYGDPEQFEVPPYPKGSGAIPGTQMRVAELAAVEPALAGLEQSLATYPPGFV
                     SSLIRAIFLCGGLLLDGAEAGGTYGPAWLVLVATPTVGERGIFQTARLGVHHELSSLV
                     WNRSPQIQLRWRALLPTGWSPARTAKEALGAYEGDGKTPHDGFLSSYGATTEENDFNV
                     YAETAFTEAAELVKLAQSDATIAAKVALLIQAYEQIDARMGAVFQRLSLARFRRDQAD
                     SGTTVFSVPAPLVPTGQVVDLRE"
     gene            3083..3871
                     /locus_tag="VFQ61_20935"
     CDS             3083..3871
                     /locus_tag="VFQ61_20935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956981.1"
                     /translation="MTENKRATVLLDGGIGLEITSRNSTVLRDEQIRLSQSLGGAGVV
                     AVELYGRAGGKAILEARSPGGMVAASIEVTVVEINRGSSAPAWIRLDGPHASLNAPNV
                     PKMQMSSTRVVPWEEGAEEILRKVPRGARHLVFNCHGFRAGKKKDFPAAHLSIGTAIH
                     LGNVGAFSQLRELHELKVIWLQSCNIAGAGEGNELCQRMADASGCYVVAQEKSIPIIV
                     APANHVYDAASQTPLYFKPQSAEKVWRGDFFVLDRELGFELKRK"
     gene            3882..>4581
                     /locus_tag="VFQ61_20940"
     CDS             3882..>4581
                     /locus_tag="VFQ61_20940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956982.1"
                     /translation="MSTRKEPTIAARHARAGTLLAWAVACGPGCDDFPARTRRLNADV
                     TPTAPTQFWVVPGTALTRASTGGLGANSSEPTRLSQPTLVEWVSAGAVRPVGASDGAS
                     LLHLPEEAWSTTRKLGLALYVQRPATLFFGAPNGPEFGRAAPGALLRVAVKKNGDLPV
                     SEDRVRVGLAAYEDPAATAFIDRDALGTNFVPLHEANGVFDAPRGWLGVRYRGLRASV
                     LDLASERWREAFEAS"
BASE COUNT          846 a         1388 c         1435 g          912 t
ORIGIN      
        1 atgcgatcta cgccgctccg cagactcaac attctggcga gcaaacagta cacctcgtac
       61 agcacggcgg tcgtggggcg agaatcactg cccagccgcc caccagcagg aactctgctt
      121 tcctttcgca gagctcgatt agcagatccc accgatgatc atccatgcgc cctggacctc
      181 gcgcagcgcc gatgccagtg ccttcagcgc gtcatcaagg cgccgctcac cggttttgcg
      241 acgcacgttt cgctcgctgc tgcactcgcg cccagcgacg ccgaacgcgt tccacggcgt
      301 cctccgatac cggatcgcga gcagtgggcc agcctgcgct ccgctcgatc gcgtttgccg
      361 ctgactgaga ctcgcctacg gccgcgagcg gtcgcgctcc ttcaacggcc acgagctgct
      421 gctgtcggcg agatgctgcc gcttggccac tacgccatcg cagaaatgct gttcgttgat
      481 cgagcttgtg ggacacagat gtggagtcac cctagcagac aggaacgcct ttgtgttagg
      541 cctcggcaag cgccacgctc gcacacaggg ctttccctcc cttcaccccc cgcgcggaac
      601 catcggaaac cgaggagtcg cgcacggtag cctcgtcgag acgcaagtgc gcgctctccg
      661 tgacgcgccg aagccgtggc aggcccgtac ccctggtctg cgggtggccc gctcgccctt
      721 gcctgctcaa cgccgctatc gtgtcatggc ccgccgtagt tcagcgctcg ccgaggcgct
      781 cgagctcttc gacatctgca aggtctttga ggccgagatc ttgttggcga tgagtgccgc
      841 cttgccgatg tatgggatcg catggccttc gagctcgaac gtccccgagc cccgcagcgc
      901 ctcgtcgaac gagacgccgc tgatcttggt gagcacctcg atcttgagcg gtgcgatgcc
      961 gaatcgatag gcatcgccct ccctcgcgaa atgtcccggc gcgaccgagt gcgcacgtag
     1021 cggcgcccca aaggcctcaa gagctgcgaa gacacgttcg gagttcgtcg gggatggcct
     1081 cacgaagtct tgaggaagct tagccaaccc gttcaccaat aacgaaagtc tcgccgggcc
     1141 acgcatcgcg cggaaccttc gagaaccctc cccgaagcgc ctgatgatgt tcgatgagtc
     1201 ggacggctgc ggccagcctt tcttccggtg tgctgtccgc gtacggtggg agtttgcgct
     1261 cacccaacgc aacgacttcg acggtcatgc gcgcccgtcg cgccgccgcc ctgagcttgc
     1321 gcgcctccaa agcttcgacc tcggtcgcca tcgggtaact ttatcacgaa cgagccaacc
     1381 gtcatgccac gagcagtacg ctgaatgccg cggcaaaact cgtggtacat cggcggtgat
     1441 ccgctcgaga tgctggatcc gtttggccga tcgccgggtg cacgctcgag aaatggggcc
     1501 ttgagcttga gccgagcggt aagcgggtgc ggtcgttact cgtcgagcca ggcgaagaaa
     1561 gccgtgacgt gcggccgcaa gttcaagata atcgaacaag acatggtcgc gctcggcgag
     1621 ttggacgaga aagtgccctt tcttgcgggg ttgccgagtt cggtcattcg gaaccggctg
     1681 cacgcacatc ccggtcgcat ccgccgagat gcagaaggcg gtggccagag cgtgctctcg
     1741 catggccttt acgacggtgc gcgcacagcg gtcaccgact gtcttcttcc agcgcgacaa
     1801 cgtaccgcga ttggtcgcag atgattagag accatgacgc gtcgctctct ggcaaccttg
     1861 ggcctggccg tgttgcttgc aaccgcaggt gggctgcttc tgtttcgcaa agcagccccg
     1921 gtggatccct cggccctgcg agttcgagct acggcactat cggtaaaaca tcgaatccgc
     1981 gtggagtacg gggaccccga acagttcgag gtaccgccct atccaaaggg aagtggggca
     2041 attccgggca cgcagatgcg tgtcgctgaa ttagcagcgg tagagccggc cctcgctggc
     2101 ctcgagcagt cgctcgcgac ctatcctccc ggcttcgtgt cctccttgat tcgggcaatc
     2161 ttcctttgcg gaggtctcct tttggacggt gccgaggccg gtggcaccta cggaccagct
     2221 tggctcgtgc tcgtagccac ccccaccgtg ggggagcgcg gaatcttcca gaccgctcgt
     2281 ctcggtgttc atcacgagtt gagttccttg gtgtggaatc gatcgcccca gattcagctg
     2341 cgctggcgcg ccctgttgcc cacgggctgg tcacccgcgc gcaccgccaa agaggctctc
     2401 ggagcgtacg aaggggatgg caaaacccct cacgacgggt tcctgagcag ctacggcgcc
     2461 acgaccgaag aaaatgactt caacgtttac gccgagacgg ccttcacgga ggccgccgag
     2521 ctggtcaagc tggcccagtc cgacgcaact attgccgcga aggtggctct gttgattcag
     2581 gcttacgagc agatcgacgc gcgtatgggc gcggtgtttc agagactttc cctggcgcgt
     2641 tttcgccgcg accaggccga ttcgggcacg accgtcttct ccgttcccgc gccgctggta
     2701 cccaccgggc aagtcgtgga ccttcgcgag tagaactata gcgccctacg cgaattctgc
     2761 tcacagccag ggttcgggtc gagcggtcga gcgtcgcgct aaggctctct ttcgctgcga
     2821 cctagagatc acggagtctt cttctcaagc tctaactccg ggatagcttg tgagtggcca
     2881 gggtgacgtt tctcacgtgt gcttcgaagc cggacatgac aggcaatggc aggcgcgcac
     2941 gagccgctcc aacgcaagta cgtctgcctt gatttcgtgg aggttccatt gtcgagtaga
     3001 tgatatggtg aggttcatgc ggccggcagg cgcaactctg gatcgataca aggtgcacgg
     3061 aggggaacta gtcgccgtga ggttgacgga gaacaagcga gcgaccgtgc tactggacgg
     3121 tggaattggt ctcgagatca cttctcgcaa ctcgaccgta ttgcgtgatg agcagataag
     3181 actatcccag agcctaggcg gtgcgggagt ggtcgcggta gagttgtacg ggcgagccgg
     3241 aggcaaagct attttagaag cacggtcgcc aggtggcatg gttgcggcga gcattgaagt
     3301 taccgtggtc gagattaatc gaggaagttc cgcacccgct tggattcgtt tggatggccc
     3361 gcatgcgtct cttaatgccc ccaacgtgcc taagatgcag atgtcgtcta ctcgagttgt
     3421 tccttgggag gaaggcgcgg aagagatatt gagaaaggtg ccacgcggcg ctcggcacct
     3481 ggtcttcaac tgccacggct ttcgcgcggg gaagaagaag gactttccgg cggcgcacct
     3541 gagtatcgga acggcaatcc acctgggaaa cgtcggtgct ttttcgcaac tgcgagagtt
     3601 gcacgagctg aaggttatct ggctgcaatc gtgcaacatt gccggagccg gggagggtaa
     3661 cgagctttgc caaagaatgg ctgatgcatc cggctgttat gtcgtggccc aggagaagtc
     3721 gattccaatc atcgtcgcgc ccgccaatca tgtctacgat gctgctagtc agactccact
     3781 gtatttcaaa ccccagagtg cggaaaaggt ttggcgcggc gatttctttg tcctcgatcg
     3841 agagctaggg tttgagctca aacgcaaata gggtacctga cgtgtccact cgcaaggagc
     3901 caacgatagc agcgcgacac gcgcgcgcgg gcacgctgtt ggcttgggca gttgcgtgcg
     3961 gtcccggctg tgatgatttt cccgcgcgta cgcggcggct gaacgcggac gtcacgccga
     4021 cagctccaac gcagttttgg gtggtgccgg gtactgcgct gacgagggcg tcaacgggag
     4081 ggctcggtgc caactcgtcg gagcccacgc gattgtcgca gcctaccctc gtagaatggg
     4141 tatccgccgg agcagttcga ccggtggggg cgagcgatgg cgcgagcctc ttgcacctgc
     4201 cagaggaagc ctggtctaca actaggaagc tggggcttgc gctttacgtt caacgtcctg
     4261 caaccctatt ttttggtgct ccgaatggcc ctgaattcgg aagagcggcg cctggcgccc
     4321 tcttgagagt tgccgtcaag aagaatgggg acctgccggt ctcggaggac agggtgcgcg
     4381 taggccttgc cgcatacgag gatcccgcgg caacggcgtt catcgatcgg gacgcactcg
     4441 gaaccaactt cgtccccctc catgaagcga atggggtttt cgatgcgcct cggggatggc
     4501 taggagttcg ttatcgtggt ctccgtgcat cggtactcga tctcgcatcg gaacgctgga
     4561 gggaagcctt cgaggcttcg c
//
LOCUS       DASTKU010000598         4231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1456376, whole genome shotgun sequence.
ACCESSION   DASTKU010000598 DASTKU010000000
VERSION     DASTKU010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456376"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            963..1922
                     /locus_tag="VFQ61_20945"
     CDS             963..1922
                     /locus_tag="VFQ61_20945"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9956983.1"
                     /translation="MAHAVQDTQLARAASASAITTPETFARPSSAGSGACNTEHETHD
                     APPVSIDPSLGADRPSGVAAKIDSPLRVDSPLRVDSPPAGAPNMPSSGALPGSDALGG
                     SERLRGSEALPGSNALAGAASSESGISSTAADTHVGDMAVAEVDVAAQAQSANDKNSA
                     ARNLLTTGDMARLSSSTLRTVRFYEEEGILEPAVRGESGHRLFPKSELDKLRLVGELR
                     LLGLPLDEIKTLFELKRTSPSGGQAACDALLRLETYLERVRERIDRLRSLEREIEDAC
                     AAIRRCQGCTDNHLFPESCGECRTMKDAGDLQPATTVLWGLKR"
     gene            1853..2374
                     /locus_tag="VFQ61_20950"
     CDS             1853..2374
                     /locus_tag="VFQ61_20950"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HET9956984.1"
                     /translation="MSNHEGRRRSAAGHDSAVGPEALKREAAVSSPRVFTIPEVNALI
                     PTLNLLVAQQLRQRSDIEQGLAELTRLSRETPLELAVHPTDSAELRRLKQDLRHRIRG
                     YEAGWNRIRELGGVIKDAQIGLVDFYGRIDGRTVWLCWRYGEDSLGYYHEIEEGYSGR
                     RPLSANVRKHTLN"
     gene            2526..3152
                     /locus_tag="VFQ61_20955"
     CDS             2526..3152
                     /locus_tag="VFQ61_20955"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HET9956985.1"
                     /translation="MTAKLKPNDVVRAVNRERATRAIAMFLEALGYDLTREDLIETPN
                     RVVAAYADELLIGDQTDLAGLIVEGSQSLEGELEAAPPGLVLVKDITVAAVCPHHLLP
                     ALGRASVAYLPGKRLLGLGTVAHVVDACARGLALQESIGERVVQALMTHAGARGALCR
                     LELQHTCMAARGAKQAESQLVTLARGGALAESSSDAELLLAFSSEMKP"
     gene            3149..3835
                     /locus_tag="VFQ61_20960"
     CDS             3149..3835
                     /locus_tag="VFQ61_20960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9956986.1"
                     /translation="MSVCVVTGASRGIGRACALALAARRKSLALLGRDSLALRQTLAD
                     CLEHGAPAAHFVECDLAEPVQIEQAAKSLLERCAAPEVLIHNAGIVERGAIGELALAS
                     FQAQLAVNLTAPFCLTNHVLPAMRAEGRGRVLFVASISSTLGTKNQSAYCASKWGVLG
                     LMKSLAQELSDTGLMTAAVLPGSVDTDMLHGSGFAPRMSAAHVAQTLVYLALDAPLAH
                     NGATIEMFGT"
     gene            complement(3933..4020)
                     /gene="ffs"
                     /locus_tag="VFQ61_20965"
     ncRNA           complement(3933..4020)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFQ61_20965"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT          819 a         1280 c         1369 g          763 t
ORIGIN      
        1 ggccggggct gccctgccat gccgaaaggc taattcactg agcgcgcgaa tatcgcggac
       61 cgccgacaag cgcgaagcaa gcggaaagaa cgttcgttgc ccgcggcgcg gcgtcgattc
      121 ggccgcggct ggctcgagat agccatacct gggattgccc ggcgcggatc actcgcaata
      181 agctaggagt cattcgtgca ccgagtgcac ctttcccgcg ttccttaaag caggctcgag
      241 gcacaggccc cccgcggcgg ccgcagtgag catgccagca aagaagcgga cagatctcgc
      301 caactgcttg ctttcgctac acttccagcc ctgacgcaga ctcacgctcc gctctgcgga
      361 cggaggggtg tgatggatga tgagtgcagc tagccagtca gcgtgaagaa ggcttggatt
      421 tcacaccgcg agcaaagccc gacgctgcac ttggatcgca cgttggctgg cctcgcccaa
      481 ctccaggaag cactctggtt ccggctccgg acgcgcgaag catgaaattg aaggagatca
      541 gagaagcatc caaagagagc tgcagagcgg acaacatgga ttgcgggatt tgagtgattg
      601 gaaggacgcg aaggctcagg aaggatcagg gaagattcga agaattctgg ggattggcag
      661 aagtgaagaa ttgggagcag tcaagcaagt cacgagcacc aggtggttcg ccgattgctc
      721 ctgggccgcc gtgcgacatc ccctcagcgg agccccgata gcacgcgctg actcaaagtc
      781 gattgtgtca tgttcaagtg gcatcacctt gaccgatcga tatccgcttt ctaagctcca
      841 tgcgcttgtc caacggaagg gaagctacga gatcggagcc cacgagggac gcggcgcgtt
      901 cgacgcatcc tgtccaggtc gatctcgcgc cggcagatct agccgcccac atggttcatc
      961 ctatggcgca tgcggttcaa gatacacagc tagcgcgcgc tgcgagcgcg agcgcaatca
     1021 ccacgccgga aaccttcgcg cggccaagct ccgcgggctc gggcgcgtgc aacacagaac
     1081 acgaaaccca cgacgctccc ccggtctcga ttgacccaag cttgggcgcc gatcgaccgt
     1141 ctggcgtcgc ggcaaagatc gattcaccac tgcgagtcga ttcaccacta cgagtcgatt
     1201 caccgccggc gggtgcgcca aatatgccta gctccggcgc actgccgggc tcagatgcac
     1261 tgggaggctc ggaaagactg cggggctcgg aagcactgcc gggctcgaac gcactggcag
     1321 gtgcagccag tagtgagagc ggcattagca gcaccgccgc cgacacgcac gtcggagaca
     1381 tggcggtcgc tgaggtcgac gtagcagcgc aggctcaaag cgcaaacgac aagaacagcg
     1441 cggcgcgcaa tcttttgacg accggagaca tggctcgcct ctcgagtagc acactgcgta
     1501 cggtgcggtt ctacgaggag gaaggcatcc tggagccggc ggtgcggggc gagagtgggc
     1561 accgcctgtt tcccaaatcc gagctcgaca aactgcgtct cgtgggggaa cttagattac
     1621 tcggtctacc gctcgacgaa atcaagacgc tgttcgaact gaagagaacg agcccatcgg
     1681 gtgggcaagc agcatgtgat gcgctgctac gcctcgagac ttacttggaa cgtgtgcgtg
     1741 aacgcatcga tcgcctgcgc agcttggaac gcgagatcga ggacgcttgt gccgcgatcc
     1801 gccgctgtca gggctgcacc gacaatcacc tgtttccgga gagttgtgga gaatgtcgaa
     1861 ccatgaagga cgccggcgat ctgcagccgg ccacgacagt gctgtggggc ctgaagcgct
     1921 aaagcgagag gccgccgtaa gcagtccgcg cgtcttcacc attcccgaag tcaatgcgct
     1981 gattccgacc ctgaatcttc tggttgcgca acagcttcgg caacgcagcg acatcgagca
     2041 agggctggcg gaattgacac gtttatcgcg tgagaccccg ctcgaactcg ccgtgcaccc
     2101 gactgattct gcggaattgc gccgtctgaa acaggacctg cggcaccgca ttcgcggcta
     2161 tgaggcagga tggaatcgga tccgcgagct cgggggcgtg atcaaggatg cgcaaatcgg
     2221 cctcgtcgat ttctacggtc gcatcgatgg ccgcacggtt tggctgtgct ggcgctatgg
     2281 cgaagattcg ctcggctact atcacgagat cgaagaaggc tattcggggc ggcgtccact
     2341 cagtgccaat gtgcgcaagc acacactgaa ctgatcggga tgaggcgctc accgagtgcg
     2401 gagcgcggac gagcagggaa gcaactgacg cgcgatcacc cgcgatcgga cgcgcgctgt
     2461 gggtttgcgt cttggatttg gcttgacgac ggctacgggc gcgggttccg tcgcggtcaa
     2521 gatccgtgac cgccaagctc aaacccaacg atgtcgttcg cgccgtcaac cgtgagcgcg
     2581 ccacgcgagc aatcgcgatg ttcctcgagg cgctcggtta cgacttgacg cgcgaggact
     2641 tgatcgagac tccgaaccga gtggtagcag cctacgccga cgagctcctg atcggggatc
     2701 agacggactt ggctggcttg atcgttgaag gctcccaaag cctagaggga gagttggagg
     2761 cggcaccgcc cgggctcgtt ctggtgaagg acatcacggt ggccgccgtc tgtccgcacc
     2821 accttctgcc cgccctgggt agagcgagcg tcgcctactt gcccggcaag cgcttgctcg
     2881 ggttgggaac cgtcgcccac gtggtcgatg cttgcgctcg cggcctggcg ctccaagagt
     2941 cgatcggcga gcgagtggta caagccttga tgactcatgc aggcgcaaga ggggccttgt
     3001 gtcgactcga gctgcagcac acgtgcatgg ccgcccgcgg cgccaaacag gccgaatcgc
     3061 agctcgttac gttggcgcgc ggaggtgcac ttgccgaatc gtctagcgac gcagagctac
     3121 tgctcgcctt ctcgagcgag atgaagccgt gagcgtttgc gtggtcaccg gcgcgagccg
     3181 cggcattggt cgagcctgtg cactcgcgct cgcagcacgc cggaagtcct tggcgttgct
     3241 tggtcgcgat tcgctggcgc tacggcagac gcttgccgat tgcctggagc acggcgcgcc
     3301 cgcggcgcac ttcgtggagt gcgacttggc ggagcccgtg cagatcgagc aggccgccaa
     3361 gagcttgctg gagcgctgcg ccgcccccga ggtgttgatt cacaacgccg ggatcgtcga
     3421 gcgcggggcg atcggtgagc ttgccttggc tagcttccag gctcagttgg cggtgaacct
     3481 gacggcaccg ttttgtctaa ccaatcatgt gttgccggcc atgcgcgccg aagggcgcgg
     3541 ccgtgtacta ttcgtggcca gcatctcgtc gacgctcggg accaagaatc agagcgctta
     3601 ctgcgcgagc aaatggggcg tgctcggcct gatgaagagc ttggcgcaag agctcagcga
     3661 cacgggcttg atgaccgcgg ccgttctgcc cggatcggtg gacaccgaca tgcttcacgg
     3721 cagcgggttt gccccgcgca tgagcgccgc gcacgtcgcc caaactctgg tctatctcgc
     3781 actcgatgcc ccgctcgcgc acaacggggc tacgatcgag atgttcggca cgtgacactt
     3841 ggcgctgccg cgtcttcaag taccctacgg cgttctcgac cagcggccgc aggctaaaag
     3901 ctcgcggcga actcgcggcg tcggatcgcg ttattggagg gcccgccaca cccgcttgtc
     3961 tttaccgttg ctcccttccg ggcctggcgg ggttcaagct gcgctgtcgg cggacccata
     4021 tgtgcgcgaa acgcggcgtg agtagtagca gggcccgtcg ggttcgacaa gctttttgcc
     4081 gaggatggct ctcgacgcgg agtttttgag cgcgaatact gcttccatga ggtctgcttt
     4141 cctgttgtcc gttcggcacc cgagaccgcg atcgctgtgt tcatgagttt ccaggcctta
     4201 tgagttcccc gcgagaccgc cctcagaatc c
//
LOCUS       DASTKU010000599         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_339939, whole genome shotgun sequence.
ACCESSION   DASTKU010000599 DASTKU010000000
VERSION     DASTKU010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339939"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1238)
                     /locus_tag="VFQ61_20970"
     CDS             complement(129..1238)
                     /locus_tag="VFQ61_20970"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET9956987.1"
                     /translation="MRIDVWSYVDEYAAEKADILDAVQKVFASGRLILGESVARFEEE
                     FARYCDNRFGVGVDNGTNAIVLGLRALGVEAGDEVITVSNTAAPTIVAITALGAIPRF
                     VDVNPEDFLMDVNQIERAITPKTRCIVPVHLFGQCVDMDAVNQIAARHGLKVLEDCAQ
                     SHGATYKGKKAGSMSDAAAFSFYPTKVLGCYGDGGMVVTRDENVARRARRLRFYGMED
                     RFYVVEPGAYNSRLDEVQAEILRRKLTRLESYIEARRAIAARYREAFAGTDLLCPMET
                     AANRHVYYVYVVRHPKRDELLAELQRRDIHLNVSYRWPAHTMSGFTRICGNSVSLPHT
                     EALANQIFSLPMYPAFSANQQSRTCQTLLELLRKV"
     gene            complement(1235..2251)
                     /locus_tag="VFQ61_20975"
     CDS             complement(1235..2251)
                     /locus_tag="VFQ61_20975"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9956988.1"
                     /translation="MSGLAELAPTRSWRCLVTGAAGFLGGAMVRRLRAEGCFVRATSR
                     RPLPWAESEGLETVSCDLLSLDACRALLEGIDTVFHFAAPTSAVLSARDPDGDRRQHL
                     EPLVNLARASRPSDSRVMLFAGTVTQAGLPQRLPLDEAHLDQPATFYDAHKLLAEYYV
                     RLFATAGAWSGISLRLPTVYGPGASVAARDRGVVNAMIRRALRGQELPIYESGDWLRD
                     FLFVDDAISAFIAAAHHAHARAGFEVFTIGAQSLALFRAFELVASSVAELTSVRSALR
                     SVPLPEFANAVDTASYSVDSSRFTSQTGWQPRVEFSSGVRRTIEAFLSEVERPQSDYG
                     RTPG"
     gene            complement(2248..2469)
                     /locus_tag="VFQ61_20980"
     CDS             complement(2248..2469)
                     /locus_tag="VFQ61_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="HET9956989.1"
                     /translation="MSTLDPNGVYLVVINDEEQYSIWPASQNIPAGWRVEGARGTREE
                     CLDHIEQVWTDMRPRSLRDWLAAQGNVSS"
BASE COUNT          515 a          862 c          792 g          459 t
ORIGIN      
        1 acaggatact gcgggtctca tgcgatccac tcacgcaaac gcttcgttca aagcccgtcc
       61 agttcgagtc tgtccagttc gagtcgcgcg cggctactcg gctccgcggc gcgcgactcg
      121 atcctgagtc acaccttccg cagcagctcg agcagagttt ggcacgtccg gctctgctga
      181 ttcgcagaga aggcaggata catggggagc gagaagattt ggtttgccaa cgcttccgtg
      241 tgcggcaggg acacgctgtt gccgcagatt cgagtgaagc ccgacatcgt gtgcgcgggc
      301 caccgatagc tgacattcag gtgaatatcg cgacgctgca gctccgccaa tagttcgtca
      361 cgcttcgggt gccgcaccac gtacacataa tagacgtgtc gattggccgc cgtctccatt
      421 gggcacagca aatccgtgcc ggcgaaagcc tcacggtagc gggccgcaat ggctctgcgc
      481 gcttcgatat agctctcgag tcgggtcagt ttgcggcgca agatctcggc ttggacttcg
      541 tcgaggcgcg agttgtaggc gccaggctcc accacataaa agcgatcctc catgccgtaa
      601 aagcgtagcc gacgcgcccg acgggccacg ttttcgtctc gcgtcaccac catgccgcca
      661 tcgccgtagc agccgagcac cttggtcgga tagaaagaaa aagccgccgc gtccgacatc
      721 gagcccgctt ttttgccctt gtaggtcgcg ccgtggctct gggcgcaatc ttcgagcacc
      781 ttcaacccat gccgcgcggc gatctggttg acggcatcca tgtcgacgca ttgaccgaac
      841 aggtgcacgg ggacgatgca gcgagtcttg ggcgtaatgg ctcgctcgat ctgatttacg
      901 tccatcagga agtcttcggg attcacgtcg acgaagcggg ggattgcgcc gagcgcagtg
      961 atcgccacta tggtcggcgc ggccgtgttg gatacggtga tgacctcgtc cccggcctcg
     1021 acgcccaacg ctcgcagccc gagaacgatg gcgttggtgc cgttgtcgac gccaaccccg
     1081 aaccgattgt cgcagtagcg cgcgaactcc tcttcgaacc gcgccacgct ctcgccgagg
     1141 atcaggcgcc cggaggcgaa gaccttctgc acggcatcca aaatgtctgc cttctcggcg
     1201 gcgtattcgt ccacataaga ccaaacatcg attctcatcc tggagtcctc ccgtaatcac
     1261 tctgcggccg ctccacttcg ctgaggaacg cctcgatggt cctccgcacg ccagagctga
     1321 attccacccg aggttgccag cccgtttgcg acgtaaaccg actcgagtcc acgctgtagc
     1381 tcgccgtgtc taccgcgttg gcaaattcgg ggaggggtac agagcggagg gccgagcgaa
     1441 ccgaggtcag ctcggcgaca ctcgaagcca ccagctcgaa agctctgaac agagcgaggc
     1501 tctgggcgcc aatcgtgaag acctcgaacc ctgctcgagc gtgggcgtgg tgcgccgcgg
     1561 cgatgaacgc cgaaatggcg tcatcgacga acaggaagtc ccgcagccag tcccccgact
     1621 cataaatagg taactcctgc ccgcggagcg cgcggcgaat catggcgttg accacgcctc
     1681 gatctcgcgc cgcaacgctc gcgccaggcc cgtaaaccgt aggcagacgc aacgagatcc
     1741 ccgaccatgc tcccgccgtt gcgaacagcc gtacgtagta ctccgccaag agcttgtgcg
     1801 catcgtagaa ggtagcgggc tggtcgaggt gtgcctcgtc caacgggagt cgctgcggga
     1861 gccccgcctg cgttaccgtg cccgcaaaga gcatgacccg tgagtcgctc ggtctactcg
     1921 cgcgcgccag gttcacgagg ggctcgagat gctgtcgccg atccccatcc ggatcgcgcg
     1981 ccgacagaac cgcgctagtg ggggctgcaa agtgaaacac ggtgtcaatc ccctcgagca
     2041 gagcgcggca ggcatcgagc gaaaggaggt cacaagatac cgtctccagg ccttcagact
     2101 cggcccaggg caggggacga cgcgaggtgg cacgcacgaa gcagccctcg gcgcggagcc
     2161 gacgaaccat cgccccaccc aagaaaccgg ccgctccggt caccaaacag cgccaggagc
     2221 gcgtcggcgc tagctcggcg agtccgctca cgagctcacg ttgccctgcg ccgcaagcca
     2281 gtcacgcaac gaccgcggac gcatgtcggt ccacacctgc tcgatatggt ccaggcattc
     2341 ttctcgcgtg ccgcgcgcgc cctccacccg ccagcccgcc gggatgttct gtgacgcggg
     2401 ccagatggag tactgttctt catcgttgat cactacgagg tagacgccat tgggatccag
     2461 agtgctcatg tttgctctcc cgtggcccga gctccccctg cgctcagcag cttgccaatc
     2521 gcgtcgagag ttcggaaatt ctccgggatg aactggtcgt tggagacgcg aattcggaaa
     2581 cgcccctcca agaaggcaac caagcgcacg acggacatcg agtccaag
//
LOCUS       DASTKU010000600         5437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_339951, whole genome shotgun sequence.
ACCESSION   DASTKU010000600 DASTKU010000000
VERSION     DASTKU010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339951"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFQ61_20985"
     CDS             complement(<1..303)
                     /locus_tag="VFQ61_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956990.1"
                     /translation="MKATPLLTRIFAWGFALGAATLTSEVHADRPAPLSAQTLKAPKG
                     PSALRGLGESFSANPATGTGSTSVPISLPPGLVAPMLALNYVGGSGRAELGVGFTLP"
     gene            complement(324..5414)
                     /locus_tag="VFQ61_20990"
     CDS             complement(324..5414)
                     /locus_tag="VFQ61_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956991.1"
                     /translation="MLLLTALCLLTPARRALADACIPTRGQACTTSSTCSGAASACIN
                     SSCQVPCGVVPSGQLDERPLPNLCSIGESCVAGTAGAKQRYFCKPTPFRMDLNLLDSC
                     IYHFVQGVDPAIGAGNECSVMRQLTNLLDQDGDSSFDIYDVDLCIKSFLGDPPCDVAT
                     QTCPNQQTYCAADAACGEGLFCNQQMHRCERECGFIVDRDFTNVPKVDRVCAGRLEAC
                     NYERGRCAEVSLEGATCQLDRDCPVGAYCLVGQCQPRCYRTLDCPDSNWFCSVDNTCL
                     PKPKGPATGEPFNPKDYSIVYAFDNIALDPFQNQYSVPLLIMNLKTGKQVFADPKVVF
                     GYRLEAKYERKEDARCSGDLTQLTTTQKEDCIVSPDEEFLTLGNPFGSLYAEGDPSTF
                     LRVNDAGANKLSPGSYPVNLTAYFSNGKSTTVRVTFKKPSPSGEYAGRMSAYLGSPEA
                     LLSTTNVAMRLYVKTKEEDTNASLVTWSSVLAANNLTREPEFEDLTEGYPVEGYIHAG
                     ESLVFDKPTATVAGENEVPVKGLYSPQLGRMRLVAVVELPKNYCRNSAGQPCTGAADE
                     WRADSAFGRDIRRIIEFIGPFKPAQRLFEGMYRETISGLMPNAFTVEGGFRITQVNQD
                     SRALNLTAPLIAAEGRVQFPDKATLVQTLKTSMEQACGASVANQLATKAAVDAYLTSY
                     GSAPPAFPGVRFEDRIQNAINTVHDATATLTLTEFLTGQVLFCSSTVTTNCVDQKQLA
                     CGLSFFRRAILEAASPDSLPDSDPNHVPGWFDREALGRDSAGHELAPPLFCNRSSTPG
                     QNTCADPNFVSSRGAVMFQDHMRFYRELTQTYTYQANAAWSDAFHALYKASERADALG
                     QAGAFAYKEGKLREVLRNYDAVRTEMLGPVASGTLFNWPMQRFQGQGDAWIKQMQTVL
                     KDRLDAWIELADLKRRVLRTADLEKSYTFAKHVIHQEYLTQVFLAELQRRWQGLNFQY
                     AGQSLTMLNSGDVFLAKLSDSRNPLGLSPDRVYFENSTPGLTNWQNYKTLVDERVTRA
                     KGTVQTAIANLHDALESKIQLDSGLMQTAQGRDASLDDICGPPGTTEPSCRLDPSDKE
                     LATACVGPNCPYQWSCDAADESGRCSNVVKLFSSAVDNVSCRSDIDSKRYDVRIGKDS
                     TTGAPIMRSCDRGRVGGLLQERVKLDLQRKQAKDKVESLLRQIAREEQELADTRASNS
                     QFIEYLNQQRTKFLAAESGVAVAEAAYEAARIGAESVSCLVIIGFANGTDCPQRGISA
                     ALQIAATAAKYAVVPALNIAMSDMSRAKEIAYQERSADAEVRQLRLRLDNMVAEVENI
                     IAEYQLLIQQLYSLDMQISDTVKTAQRTADRYAEQTNDVIKRLLGNDSSQTLRSNQLV
                     RAADAEFQNLLVFAYKMTRAFLHRYNYADQAEVWTNRVYQLITIADVEKLVTDLVEEE
                     RRYCGSQGIDCDSINGRQVMRFSVRDQLFPQLSDIVDPTTGRILFKGEQFHNLITSSA
                     FRHRRVVQGVTLENIELPFSIWANDLGTVNGAPQPVMLPRGSCNHIVAADRSDGAKTF
                     AANVMGTRLEVRAPLRYELIRGGTDQIRRCDPGGVFVSTFKVDYPLVNSSTQRAFQTR
                     TGPLEACTNNFLLEQVETRDQPQPCWNPFARDRSLAAPDWILSIPLDGQQWVVGIDDA
                     TGAPISDSEKPIVENFVLYFRYNARPSN"
BASE COUNT          974 a         1691 c         1680 g         1092 t
ORIGIN      
        1 gggcagcgtg aaccccacgc cgagctcggc tcggcccgaa ccgccgacgt agttcagcgc
       61 cagcatcggc gcgactaacc cgggagggag cgaaatcgga actgacgtgc tgcccgtccc
      121 cgttgcggga ttggctgaga acgactcccc gagtccgcgc agcgctgatg gccctttggg
      181 ggccttcagc gtctgcgcgc tcagcggcgc cggtcggtcc gcatgaacct ctgatgtcag
      241 agtcgccgcc ccgagagcaa agccccaggc gaagatccgc gttaagagcg gcgtggcttt
      301 cacacccaac ctccgcgacc ggctcagttc gaaggccgcg cgttgtatcg gaagtagagg
      361 acgaagttct ccacgatcgg cttttcgctg tccgagatcg gagcgcccgt cgcgtcgtcg
      421 atgcccacca cccactgctg gccgtctaga ggaatgctca ggatccagtc gggggcagcc
      481 aagcttcgat cacgggcgaa ggggttccaa cagggttgtg gctgatcccg ggtctccacc
      541 tgctcgagca ggaagttgtt ggtacatgcc tccagcggcc cggttcgcgt ctgaaacgcg
      601 cgctgggtgc tggagttcac gagcgggtag tcgaccttga aggtactcac gaacacaccg
      661 cccgggtcgc agcgacggat ctggtcggtg ccccctcgga tgagctcgta gcgaagcggt
      721 gctcggactt ccagccgcgt gcccatgacg ttcgccgcga acgtcttcgc gccgtcggag
      781 cggtcggccg cgacgatatg attgcaagag ccgcgcggca gcatgacggg ctgcggggcc
      841 ccattcaccg tgccgagatc gttcgcccaa atcgagaagg gcaactcgat gttctcgagc
      901 gtaaccccct gcactacgcg cctatgccgg aatgcactcg atgtgatgag attgtggaac
      961 tgctcgcctt tgaacagaat tcgccccgtc gtcggatcga cgatgtccga gagctgcggg
     1021 aagagctgat cccggaccga gaagcgcatc acttgccgcc cgttgatcga gtcgcaatcg
     1081 atgccctgac tgccgcaata gcgcctttcc tcttccacca gatccgtgac gagcttctcg
     1141 acgtcggcaa tcgtgatcag ctgatagacc cggttggtcc acacctccgc ctgatcggcg
     1201 tagttgtagc ggtgcaggaa ggcccgcgtc atcttgtagg cgaaaacgag cagattctgg
     1261 aactctgcgt ccgcggcgcg aacgagctga ttggaacgca gggtctggct gctgtcatta
     1321 ccgagcagcc gcttgatgac gtcgttcgtc tgctccgcat agcgatccgc ggtgcgctgg
     1381 gccgtcttca ccgtatcgga gatctgcatg tccaggctgt agagctgctg aatcaacagc
     1441 tggtactcgg cgatgatgtt ctcgacctca gccaccatgt tgtcgagccg cagccggagc
     1501 tgccgcacct ccgcgtccgc gctccgttct tggtatgcaa tctccttggc acgcgacatg
     1561 tcgctcatcg cgatattgag ggcgggaacg accgcgtact tcgcggcggt cgccgcgatc
     1621 tgcagggcgg ccgaaatccc tcgttgcggg cagtccgtgc cgttggcgaa gccgatgatc
     1681 accaagcagc tcacgctctc tgcaccgata cgcgcggcct cgtaggccgc ctctgcgacg
     1741 gccaccccag attcggcggc aagaaacttg gtgcgctgtt gattcaggta ctcgatgaac
     1801 tggctgttgc ttgcccgggt gtccgccagc tcctgctctt cacgagcgat ctgacggagg
     1861 agtgactcga ccttgtcctt ggcttgtttg cgctgcaggt cgagcttcac tcgttcttga
     1921 agcaggcctc caacgcgacc tcgatcgcag ctccgcatta taggtgcgcc ggtcgtggag
     1981 tcctttccga tccggacgtc gtagcgcttc gaatcgatgt ccgaacggca cgagacgttg
     2041 tcgacggcgc tcgagaagag cttgaccacg ttcgagcaac gaccgctctc gtccgccgcg
     2101 tcgcagctcc attgataggg gcaattcggc cctacgcagg ccgtcgcgag ctccttgtcg
     2161 gatgggtcca gacggcacga tggctcggtc gtaccgggag gcccgcagat atcgtcgagc
     2221 gatgcgtcac gcccctgcgc cgtctgcatc aagccagagt ccaactggat cttgctctcc
     2281 agcgcatcgt ggaggttcgc gattgcggtc tgcaccgtac ccttcgcgcg cgtcactcgt
     2341 tcatcgacca gagtcttgta gttctgccag ttcgtgagtc cgggcgtgct gttttcgaag
     2401 tagacgcggt cggggctcag gccaagcgga tttcgcgagt ccgagagctt cgcgagaaac
     2461 acgtcgccgg agttcagcat cgtcaggctc tggccggcgt actggaaatt cagcccctgc
     2521 cagcggcgct ggagctccgc gagaaatacc tgcgtcaggt actcctggtg aatcacgtgc
     2581 ttcgcgaagg tatacgactt ctcgaggtcg gcggtgcgga ggactcgacg tttgaggtcc
     2641 gccagctcga tccaagcgtc cagtcgatcc ttcaagaccg tctgcatctg cttgatccaa
     2701 gcgtcgcctt gaccctgaaa gcgctgcatg ggccaattga acaaggtgcc ggaagcgaca
     2761 gggccaagca tttccgtgcg aacggcatcg tagttcctga gcacttcgcg gagcttgccc
     2821 tccttgtatg cgaacgcccc agcttgcccg agtgcgtctg cgcgctccga tgccttgtat
     2881 agggcgtgga aggcgtcgct ccaggccgcg ttcgcctgat aggtgtaagt ctgggtgagc
     2941 tcccggtaga accgcatgtg atcctggaac atcaccgcgc cacgcgaact cacgaagttc
     3001 ggatccgcgc aggtattctg acctggcgtc gaactccggt tgcagaacaa tggtggcgcc
     3061 aattcatggc cagcgctgtc tcggccgagg gcctcgcgat cgaaccaacc cggaacgtgg
     3121 ttcggatccg aatccggtag tgaatccggc gacgccgctt ccagaattgc tctccggaag
     3181 aagctgagcc cgcacgcgag ctgtttctgg tcgacgcaat tcgtcgtgac ggtcgagcta
     3241 cagaagagga cctgtcccgt caaaaactcc gtgagcgtca gagtagccgt cgcgtcgtgc
     3301 acggtgttga tcgcgttctg gatgcgatcc tcgaacctca ctcctgggaa cgccgggggt
     3361 gccgagccgt agctcgtcaa gtaggcgtct accgcggcct tggtggcgag ttggttcgcg
     3421 acgctcgcac cgcacgcttg ctccatggag gtcttcagcg tttgaacgag tgtcgctttg
     3481 tccgggaact gcacccgccc ctccgccgca atcaacggcg ccgtcaggtt cagcgcacgg
     3541 gaatcctggt tcacctgagt gatccggaac ccgccctcga ccgtgaaggc gttcggcatg
     3601 agcccagata tggtctcgcg gtacatgcct tcgaacaagc gctgggcggg cttgaatggc
     3661 cctatgaact cgatgatgcg tcggatatcg cgcccgaacg cgctatccgc gcgccactcg
     3721 tctgccgctc cggtgcaggg ctgccccgcg ctgttgcggc agtaattttt gggtagttcg
     3781 acgacggcga cgagcctcat tctcccgagc tggggtgaat agagtccctt cacggggacc
     3841 tcgttctcgc cggctaccgt ggccgtgggt ttgtcgaaca ccagactctc cccggcgtga
     3901 atatagccct ctactgggta gccctccgtc aggtcctcga attccggctc tcttgtcagg
     3961 ttgttcgccg cgagcacgct cgaccaagtc acgagcgatg cgttggtatc ctcttccttc
     4021 gtcttgacgt agaggcgcat cgcgacgttc gtcgtcgaca gcaacgcttc gggtgatccc
     4081 agataagcac tcatccggcc ggcgtactcg ccactgggcg aaggcttttt gaacgtgact
     4141 cgaacggtcg tactcttgcc gttgctgaaa tacgcggtga gatttacggg gtaagaacct
     4201 ggcgagagct tgttcgcgcc agcgtcattg acacgcaaga acgtcgaggg gtcaccttct
     4261 gcatagagac tgccaaacgg gtttccgagc gtgaggaact cctcgtcggg agagacgatg
     4321 cagtcttcct tttgggtcgt ggtgagctgc gtcagatcgc cactgcagcg tgcgtcttcc
     4381 ttacgctcgt atttcgcctc gagccggtaa ccgaacacca ccttgggatc ggcaaatacc
     4441 tgcttgccag tcttgagatt catgatcaag agcggcaccg agtactgatt ttggaatgga
     4501 tcgagcgcga tgttgtcgaa ggcgtatacg atcgaatagt ccttgggatt gaatggctcg
     4561 cccgtggccg gccctttggg cttcggcagg cacgtattat cgacgctaca gaaccaattc
     4621 gagtcgggac aatcgagcgt gcgatagcag cgcggctggc attgccccac tagacaatac
     4681 gcgcccaccg ggcaatcccg gtccagctga caggtcgccc cctccaggct aacttcggcg
     4741 cagcggccgc gctcgtagtt gcaggcctcg aggcgccccg cgcagacgcg gtcgaccttg
     4801 ggaacgttgg taaaatcgcg gtcgacgatg aagccgcact ctcgctcaca gcggtgcatc
     4861 tgctgattac agaacaagcc ctccccacag gccgcgtcgg ccgcgcaata cgtctgctgg
     4921 ttggggcacg tctgcgttgc tacgtcgcag ggcggatcac cgagaaacga cttgatgcag
     4981 agatcgacgt cgtagatatc gaagctactg tcaccatcct ggtcgagcaa gttggtgagc
     5041 tggcgcatca ccgagcactc gttgcccgcg ccgatggcgg gatctacccc ttgcacgaag
     5101 tggtaaatgc acgagtcgag cagattcagg tccatccgga acggtgtggg cttgcagaag
     5161 taccgctgct tcgcaccggc cgtgcccgcc acgcagctct caccaatcga gcacaggttg
     5221 ggcaggggcc gctcatcgag ctggccactc ggcactaccc cgcagggcac ctggcagctg
     5281 ctgttgatac atgcactggc cgctccgctg caggtgctgc tcgtggtgca tgcttgaccg
     5341 cgcgtgggga tgcaagcgtc tgctagcgca cgccgcgccg gggtcagcaa gcacagcgcg
     5401 gtgagcagta gcaccgacca gcgcgcgaat atcgctc
//
LOCUS       DASTKU010000601         5900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1008756, whole genome shotgun sequence.
ACCESSION   DASTKU010000601 DASTKU010000000
VERSION     DASTKU010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008756"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFQ61_20995"
     CDS             <1..353
                     /locus_tag="VFQ61_20995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956992.1"
                     /translation="AGTFEIRDELAPSVRMWVVADPNVAAITYPSMKGDFSLTVENAG
                     DYKVQAFFSGKRVGSELPVAVEKGDVDTTKNPIKLVDERAQAKAAKDDAEGGAEPKAD
                     ATDNKGEKPEAPAK"
     gene            447..2093
                     /locus_tag="VFQ61_21000"
     CDS             447..2093
                     /locus_tag="VFQ61_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 family protein"
                     /protein_id="HET9956993.1"
                     /translation="MLATRFWSVFVALLLGAAVFVLFVGTSMYNRAGTRTLGESLSSD
                     SQVVSWYLKDDARQRSAQLIQFTLYPDVARYLQKSNESEAKVPGEARDKLQAALKKVN
                     SQIPAAEGFDAVFAVDQHGRVVAHLGYDQATGMDNFELGGYPVVADGLRGYLRDDTLV
                     LDRIYRVVVRPVEYDTGQPPAGAIVGARIVDDRFARELSSRTGAAVAFYANGQRTASG
                     APEGFDSSQLDQIVTDLGDLEQDPDYQAKGRSKQRVLGKMLSVQYSRLPGEAWKLGAG
                     YAVARLPFSVDSPLDFFRKADDKDKEQGHIGLAIAIALLAAAAGIGFSVLEYSRPLRV
                     FRTEAQRLAKGEIDQLQPSRFQGAFRKIAADLNDGIDVLLQKGGGPRRGPADFKQVLG
                     DLPAEPVMSAFSLPGDGAPPSSTAAGAPIPPSAGPASSGRALPQPPQNRRLPKPPGGR
                     AGADAAPAAEPLSPPAASELGAWGAGGDEAAEWRAVYQEFVALKTQCGESVDGFTYEK
                     FEGTLRKTREKITQQGAKKVKFSVYVKEGKAALKASPIKE"
     gene            2133..3095
                     /gene="trxB"
                     /locus_tag="VFQ61_21005"
     CDS             2133..3095
                     /gene="trxB"
                     /locus_tag="VFQ61_21005"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET9956994.1"
                     /translation="MSEPRRHRLLIIGSGPAGLTAAIYAARANLKPICIEGFSAGGLI
                     PGGQLMFTSDVENFPGFPQAVSGQELMSRFREQAIHQGAELVTQDVQKVDFSTRPFKV
                     WAGEEENELHLADTVIIATGARANYLGLPSEHALKNKGVSACAVCDGALFRGQDVAVV
                     GGGDTAMEEATYLASLCSSVTLIHRRDDFRASQAMQERVKSNPKIKILYSHAVSEVLD
                     VSKDQVTGLRVKNLKNDAEHEISVGALFVAIGHTPMTELFKGQLDMHPNGYLRVELGT
                     TRTSVPGVFAAGDVADFTYRQAVTAAGTGCMAALDAERYLSAHE"
     gene            3437..4168
                     /locus_tag="VFQ61_21010"
     CDS             3437..4168
                     /locus_tag="VFQ61_21010"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET9956995.1"
                     /translation="MPSAQHAVLHGGSPVANAAPVPSASPPVTAAPAPSATRSEPLPN
                     AGVAAPVLDPQRRLVVLADEARHCTRCALHSTRTQAVFARGTGESGLCFVGEGPGADE
                     DAQGFPFVGAAGQLLDRMITAMGLGRDDVYVCNIVKCRPPNNRKPEPEEMAACMPFLK
                     EQLELVAPKVIVALGATAVQGLLGTTEGITRLRGKWKLYKGEIPVMPAFHPAYLLRTP
                     TAKREVWEDLQAVVRQMGRSLPERS"
     gene            complement(4274..>5900)
                     /locus_tag="VFQ61_21015"
     CDS             complement(4274..>5900)
                     /locus_tag="VFQ61_21015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956996.1"
                     /translation="IDEAINTHYLATDFDKAEAVLTGTIKACDDKCSPQTLAKAWMYV
                     GIVRGSGRNDQGGAKEAFQTALGLDPGVKLDAGLATPETQTTFQHSGGGGAVETPVAP
                     VASGGEEEASGGGEGLTCTPVVTEVETRRPVPVQCRSEAEVTNMELRYKSFGSESWKT
                     LRMEPKDGSFRAQIPCDATQNTGLLKIYVVGKNPKGKANASWGTKAQPIELSILEGST
                     AEPPSYDDADPPPRCEAKEICPPDFPGCGGNQGGTVDLGGQCQSSSQCQSGLVCMDGT
                     CESAPSCKTNDDCPAGTCEEGRCSAQGGEQMSGRARKWWVGLHVAQDIAFFGGDKVCL
                     GDAGFACYVTGENDLPYDTDYYQTKAVGRVNSGAVPGTTRVLLSIDRLLMKNITFGAR
                     LGFAFRGGPPAGKQMEWDPDGDPNTKDYLVKSDGTKFLPVHAELRGTYWFGNNPLGRR
                     GFRPYVHAGLGVGQVDAKVVVSVDDKDTVNSPVPLTADAWKKMGTMFATVGGGVVYAL
                     TPRLGIQGNLNLMLMFPDSGLNVQPSLGMVFGL"
BASE COUNT         1067 a         1866 c         1896 g         1071 t
ORIGIN      
        1 aggccggtac gttcgagatt cgtgacgaac tggcccccag cgtgaggatg tgggtggtcg
       61 cagatcccaa cgtcgccgca atcacctatc cctccatgaa gggcgatttc tctctgaccg
      121 tcgagaacgc gggggactac aaagtgcagg ccttcttttc gggcaagcgg gttggcagcg
      181 agctacccgt ggcggtcgag aagggtgacg tagatacgac gaagaatccc atcaagctcg
      241 tcgatgaacg cgcccaggcc aaggccgcca aggatgacgc cgagggcggc gccgagccca
      301 aagcagacgc cactgacaac aagggcgaaa agccagaggc gccggccaaa tgagccgcgc
      361 atctcggtcc gtgggtcgcg gagcgcaggc gcaacgcagg ctttcgattt ctaagttcga
      421 gctcgcacgt cgaggaaatt agcaagatgc tggctacacg gttttggagc gtgttcgtcg
      481 cgctgttgct gggcgcggcg gtgttcgttt tgttcgtcgg aaccagcatg tacaaccgcg
      541 cgggcacgcg gacgctgggc gagtcattgt cttcggatag ccaagtcgtc tcctggtacc
      601 tcaaggacga cgcgcgacag cgttcggcgc agctgattca atttacgctt tacccggacg
      661 tcgcccgcta cttgcagaag tcgaacgagt ccgaggccaa ggtgccgggg gaggcgcggg
      721 acaaactgca ggcggcgttg aagaaggtga actcgcagat ccctgccgca gagggcttcg
      781 acgcggtgtt cgccgtcgat cagcatgggc gcgtggtcgc ccacctcggc tacgatcaag
      841 cgacggggat ggataacttc gaactcggtg gttatccggt cgtggccgac ggtctgcgtg
      901 gctacttgcg tgatgacacg ctcgtgctcg accggatcta ccgcgtcgtg gtgcgacccg
      961 tggagtacga tacgggacaa cccccggcgg gggcgatcgt cggagcgcgc atcgtggatg
     1021 atcgctttgc tcgcgagctg tcgtcgcgca cgggcgcggc ggtcgcgttc tacgcgaacg
     1081 gtcagcgcac ggcgtcgggc gcaccggagg gtttcgattc ttcgcagctc gatcaaatcg
     1141 tcacggattt gggggatctc gagcaggatc ctgactatca ggcgaagggc cgcagcaagc
     1201 agcgcgtgtt gggcaagatg ctgagcgttc aatactctcg cttgcccggt gaagcgtgga
     1261 agctcggcgc gggctacgcc gtcgctcgct tgccgttctc ggtcgatagt ccgctcgact
     1321 tcttccgtaa ggcggacgac aaggacaagg agcaggggca catcggtcta gccatcgcga
     1381 tcgcgctgtt ggccgccgcg gccgggattg gcttcagtgt gctcgaatac tcgcgacctc
     1441 tgcgggtgtt ccgtacggag gcgcagcgtc tggccaaggg tgagatcgat caactgcagc
     1501 ccagccggtt ccaaggcgcg tttcgcaaga tcgccgccga cttgaacgat ggtatcgacg
     1561 ttctcttgca gaagggtggc gggccgcgcc gcggtcctgc cgacttcaag caggtgctgg
     1621 gcgacttgcc ggctgagccc gtgatgagtg ctttcagttt gccgggcgac ggcgctccgc
     1681 cgtcttcgac ggccgcgggt gccccgatcc cgccgtcagc cggtcctgcg tcgtcggggc
     1741 gggcgctgcc gcaaccacct cagaaccgac gtttgcccaa gccgcccggc ggtcgtgcag
     1801 gcgcggatgc cgctcccgcc gcggagccgc tctcgcctcc cgcggcaagc gagctgggcg
     1861 cctggggtgc tgggggtgat gaagctgccg agtggcgcgc cgtctaccag gagttcgtgg
     1921 cgttgaagac ccagtgcggt gaaagtgtgg acggcttcac gtacgagaag ttcgaaggga
     1981 ccttgcgcaa gacgcgcgaa aagatcactc aacagggtgc gaagaaggtc aagttcagcg
     2041 tctacgtcaa agaaggcaaa gccgcgctca aggccagtcc tatcaaggaa tgacttccgt
     2101 tagagcaatc gaagcacgtc gaggaacgat ttatgagtga accgcgccga caccgacttt
     2161 tgatcattgg ctctggcccc gcagggctga cggcggccat ctacgctgct cgcgcaaacc
     2221 tcaagccgat ttgcatcgag ggcttcagcg ccggcgggtt gattcccggt gggcaattga
     2281 tgttcacgag cgacgtcgag aatttccccg ggtttccgca ggccgtatct gggcaggagc
     2341 tgatgtctcg gtttcgcgag caagccatcc accagggggc tgagctcgtg acgcaagacg
     2401 tgcagaaggt cgatttctcg acgcggccct tcaaggtctg ggcgggcgaa gaggagaacg
     2461 agctgcacct ggccgacacc gtgattatcg ccacgggcgc gcgcgccaat tacttgggat
     2521 tgccgagcga gcacgcgctc aagaacaagg gcgtgagcgc gtgcgcggta tgtgatggtg
     2581 cgctgttccg gggccaagac gtggcggtgg tcggaggagg tgacaccgcg atggaggaag
     2641 cgacctacct ggcgagcttg tgctccagcg taacgctcat ccatcgccga gatgacttcc
     2701 gcgcatcgca agccatgcag gagcgggtca agtcgaatcc caagatcaag atcctgtata
     2761 gccacgccgt gagcgaggta ttggacgttt ccaaggacca agtgaccggt ctgcgcgtga
     2821 agaacctcaa gaacgacgcc gagcacgaga tctcggtcgg ggctctattc gttgcgatcg
     2881 ggcacacgcc gatgaccgag ctcttcaaag ggcagctcga catgcatccg aacggatatc
     2941 tgcgtgtcga gttgggcacc acgcgcacca gcgtgcccgg tgtcttcgcg gcgggagacg
     3001 tggccgactt cacgtatcgc caggcggtga ccgcggccgg cacgggctgc atggcggcct
     3061 tggacgccga gcgctatcta tcagcgcacg agtgatgagc acgcaagagc cccctccgga
     3121 agaggcgtct ctccacggag agggctcggt tcacccgctg tcggtggagg acgagctgtg
     3181 ggagctatcg cgggcactcc gagctcacct cgaatggcag cgaagcagcg gcgcgcgcgc
     3241 gttgccgaaa gatcctgccc ccgcggctcc cattgcaccg aacacgccga ctcgacagtt
     3301 gcccagggcg cccgagcctg cccggaaagt cgaaccacct tcccgtgact cggcgcgaac
     3361 ggagcgaatc cccagcgtaa gcgaaacgcc cgtggggagc gcgccttccg ccccccactc
     3421 gagcacttcc gtccccgtgc cgagcgcgca gcacgcggtg ttgcatggtg gttcaccggt
     3481 tgcgaacgct gcaccggttc ccagcgcttc tccgcccgtg accgcagcgc cggcaccgag
     3541 tgcaaccaga agtgaaccct taccaaacgc gggggttgcc gctccagtat tggatccgca
     3601 acggcgcctc gtggtgctgg cggatgaagc gaggcactgc acgcgatgtg cgctccacag
     3661 cacccgcacc caagctgtct tcgcgcgcgg caccggcgag tccggactct gcttcgtggg
     3721 cgagggccca ggcgcggacg aggacgctca aggcttcccc ttcgtcggcg cggccggaca
     3781 gctcctcgac cgcatgatca cggccatggg cctcggtcgc gacgacgtct acgtctgcaa
     3841 catcgtcaag tgccggccac cgaacaatcg taagcctgag ccggaggaaa tggcggcgtg
     3901 catgcccttc ctcaaagagc aactggagct cgttgctccc aaagtgatcg tcgcgctggg
     3961 ggcgaccgcc gttcaaggtc tactcggtac gaccgaaggc attacgcgtc ttcgcggcaa
     4021 gtggaagctg tacaaagggg agatccccgt tatgccggct ttccaccccg catatttgct
     4081 gcgaacgccc accgccaagc gcgaggtctg ggaggacttg caggcggtcg ttcgacaaat
     4141 gggtcgtagt ttgcccgagc gctcctgacg tccgcggcgg cgacgtgggg attgccgagg
     4201 gtctcggcca caaaagtcaa acggggtcag tttcagctga ccccgtttgc acgagcttgt
     4261 tcagcttgct cttttagagc ccgaagacca tgccgagcga cggctgcacg ttgagccccg
     4321 aatccgggaa catcagcatg agattcaggt tgccctgaat gccgagccgc ggcgtgagcg
     4381 cgtaaaccac gccgccgccc accgtcgcga acatcgtgcc catcttcttc caggcgtcgg
     4441 ccgtgagcgg gaccggtgaa ttcaccgtat ccttgtcatc gacgctcact acgaccttcg
     4501 cgtccacctg acccacaccc agcccagcgt gcacataggg gcggaaccct ctgcgaccta
     4561 gggggttgtt tccgaaccag tacgttccgc gcagctcggc gtgaacgggg aggaacttcg
     4621 ttccgtccga cttcaccagg tagtctttgg tgttgggatc accgtctgga tcccactcca
     4681 tctgcttgcc tgccggggga cctccgcgga acgcaaaacc aagcctcgcg ccaaacgtga
     4741 tgttcttcat caggagtcga tcgatgctca ggagaacgcg cgtggttcca ggcaccgcac
     4801 ctgagttgac gcgaccgacg gccttcgtct gataataatc cgtgtcgtac ggcaggtcgt
     4861 tctcgcccgt gacgtagcag gcgaaaccgg cgtcacccaa gcagaccttg tctccgccga
     4921 agaaggcaat gtcctgcgca acgtgcagac ccacccacca cttgcgggcg cgtccgctca
     4981 tttgctcgcc gccctgagcc gaacaacgac cttcctcgca cgtgccagcc gggcaatcgt
     5041 cgttcgtttt gcaactcggt gccgactcgc aggtgccgtc catgcagacg agtccggact
     5101 ggcactggct ggagctctgg cactggccgc ccaaatccac ggtaccgcct tggttaccgc
     5161 cgcagcccgg gaaatcaggc gggcagatct ccttagcctc gcagcgcggc ggcggatccg
     5221 catcatcata gctcggcggt tccgccgtcg atccctccaa gatcgagagc tcgatcggct
     5281 gcgccttggt gccccagctg gcgttcgcct tgcccttggg attcttgccg accacgtaaa
     5341 tcttgagcag ccccgtgttc tgcgtggcat cgcacgggat ttgcgcgcgg aagctgccgt
     5401 ctttgggctc catgcgcaga gtcttccagc tctccgagcc gaacgacttg tagcgcagct
     5461 ccatgttggt gacctcagcc tcgctgcggc attgcacggg cacggggcga cgcgtctcca
     5521 cttccgtcac gaccggcgtg caagtgagcc cttcaccacc tccggacgcc tcttcttctc
     5581 cgcccgacgc gaccggcgcg accggcgttt ctaccgctcc tccgccgccg gagtgctgga
     5641 acgtggtttg ggtctcgggc gtagccaagc ccgcgtcgag cttgactccc ggatccaacc
     5701 ccagcgccgt ttgaaacgcc tctttggccc caccctgatc gttgcgaccg ctgccgcgaa
     5761 cgatgcccac gtacatccaa gctttggcca gcgtttgagg gctacacttg tcgtcacaag
     5821 ccttgatcgt gccagtcagc acggcctcgg ctttgtcgaa gtcggtagcc agatagtgag
     5881 tgttgatcgc ttcgtcgatc
//
LOCUS       DASTKU010000602         4145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1903850, whole genome shotgun sequence.
ACCESSION   DASTKU010000602 DASTKU010000000
VERSION     DASTKU010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903850"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFQ61_21020"
     CDS             complement(<1..331)
                     /locus_tag="VFQ61_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9956997.1"
                     /translation="MGASEHVPGSTHQRAGADPQSSECCEGSVFQSYREQKPEKQNSG
                     QQSSLLGIFGRPYVDLEPHLDLTGLADLHDEICLGLTQVAVDYTGGSHRTLGIVPPSL
                     PDPSFADY"
     gene            427..1278
                     /locus_tag="VFQ61_21025"
     CDS             427..1278
                     /locus_tag="VFQ61_21025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HET9956998.1"
                     /translation="MPLAYVMGFGLATIVMLLVVLRVGQRLFSPAHTVARHLAEDNVA
                     QRLLSVGQVLSVFLVAGSAVKNCLEGESLLHDLLWVATFALAGLLLVLLTGRAGINLL
                     LRSRLAHEVERGNAASGLAAGAHYVATGIITSRALAGHSLRELALSLGFFVLAQVTLH
                     VFILLFRALTTYDDAEQIQGENLAAALSYSGVTIAIALVIARALEGDFAGLAVSLKGY
                     AAVLAFLFALYPVRQLLVQGVLLGAPISMRGGRLDQAIAGERNEGMGALEAATYLATA
                     LVVTRLL"
     gene            1306..2571
                     /locus_tag="VFQ61_21030"
     CDS             1306..2571
                     /locus_tag="VFQ61_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HET9956999.1"
                     /translation="MSSLANEPEQSYRDFASRVVAGGILADPWISGLPRFREEPLLVS
                     TAQQRLLYDAAEEMAFVYDELCRIVSDDPALLDDFFGLTPCQKAMWFASQPLWHGLAR
                     ADLFMTAEGPVIAELNCDTPTGEAEAVELNRLVRDQHPGTYDPNRELGPRFCALLDTV
                     ARAAFGRAPTPPLQVGLVYPTEFTEDLSLVRLYRGWLEERGYNVLLGSPYNLELEGRG
                     LSLFSASFKLLLRHYKTDWWGERSSVWSDEDIPDPEPLAEPLAAMLTASAEGQVAIIN
                     PLAAVVPQNKRAMAFMWEHLQRFSPQSQAAIQRYLPLTSRLEVIHPELLIAEREDWVL
                     KSDYGAEGEEVIIGRHTSDALFRECVAKARPGRWVAQRYFAALENERRESVNYGVYLI
                     AGEASGLYSRIQAGATDVNALSVPTLIVK"
     gene            complement(2572..3393)
                     /locus_tag="VFQ61_21035"
     CDS             complement(2572..3393)
                     /locus_tag="VFQ61_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957000.1"
                     /translation="MTTGLCGIWGEPYIDLTDHIDISCFERLDREISVGLCHVETTFT
                     GGSLKHMGVCAPWVERDPYVDYGQVISQFSREEFLEFVALGHVPQDFDPERRGEYGFG
                     DETDHPLTREQMLYLKYRYGVYFPWQICYHFLENQRWEDKHSGLNKQFDAPAREFFPK
                     TVEFLRSLPFVEIGRAVLFGLEANHHAPAHRDSEPGKSLGVAQSISFSPRGNKRFYVC
                     DPEDRERRVVDSRIYWFNDMDYHGVEPDPFFRYSIRVDGVFERGFARKLEQSRRR"
     gene            complement(3390..>4145)
                     /locus_tag="VFQ61_21040"
     CDS             complement(3390..>4145)
                     /locus_tag="VFQ61_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9957001.1"
                     /translation="FSASCRGLREVRVDVLHGFVFVCLGNDTAELGTSLAGAPEWLQG
                     SELDLLRLGRAVSYEVAANWKICVENFQESHHFERVHPGLQRQTPSAGASSWKGSGGW
                     LGGLMQLASHASTVAAEPRSAGESTEPAPKRPWIVPPEDRRRVADAFAFPTLLTSLQP
                     DYLLTYRLQPLGPARTGVVAATYFHMAAFAPEPWARDVYEFWDQVNAEDRAICERQQR
                     GVQSRSYEPACYSLVEDGVHAFDQMVARSYLQT"
BASE COUNT          755 a         1312 c         1259 g          819 t
ORIGIN      
        1 cataatcggc gaacgaagga tccggtagcg aaggcggtac gatgccgagc gttcggtgac
       61 tgccgcccgt atagtccacc gcaacctgcg tcagccccag acaaatctcg tcgtgcagat
      121 ccgccagccc ggtcaagtcc aagtgaggtt cgagatcgac gtaaggccgc ccgaagatgc
      181 ctagcaggga gctctgctgt ccggagttct gtttttcagg cttctgttct ctatagctct
      241 gaaaaacaga gccttcacag cattcagagc tctgcggatc agccccagcg cgctggtggg
      301 tggatccggg gacgtgttca cttgctccca tgacctgttc gaactaaccc gacagacttt
      361 accagaaccg acgctcttgc aaagggcctc ttgccttgca gcgctcgcgt tctgtaactt
      421 caccccatgc cgctcgccta cgtcatgggc ttcgggctcg caacgatagt gatgctgctc
      481 gtggtgctgc gcgttgggca acggctcttc tcgcccgccc acacagtggc ccgccatctc
      541 gctgaagaca acgtcgccca acgcttgctc agcgtgggtc aggtgctgag tgtcttcttg
      601 gtagcaggga gcgccgtcaa gaattgtctc gagggcgaga gcttgctgca tgatttgctg
      661 tgggtcgcaa cgtttgcatt ggcgggtctc ctgctggttc ttctgaccgg ccgcgccgga
      721 atcaacctgc tgctgcgctc gcgcctggct catgaggtcg agcgcggaaa cgcggcgtcc
      781 ggtctggccg caggagcaca ctacgttgcc accgggatca ttacctcccg cgcgctcgcc
      841 gggcacagcc taagggaact ggcgttgtcg ctgggcttct tcgtgttggc gcaagtgaca
      901 ctgcacgtgt tcatcctctt gtttcgtgcg ctcaccacgt acgatgacgc cgagcaaatc
      961 cagggcgaaa acctggcggc cgcgctcagc tatagcggcg tcacgatcgc catcgcgctg
     1021 gtgatcgccc gggccttgga gggcgacttc gcaggcctcg cggtctcgct caagggctac
     1081 gccgccgtgc tggcgttcct gttcgcgctg tatccagtgc gtcagctgct cgtgcagggc
     1141 gtgctgctcg gggcgcccat ctcgatgcgt ggcgggcggc tggaccaggc aatcgcgggc
     1201 gagcgcaatg aaggaatggg ggctctcgag gcggcgacct atctggccac cgcgctcgtc
     1261 gtcacccgtc tgctttgatg gaccgggttt attttggcga acaaagtgtc tagtttagcg
     1321 aacgagcctg agcaaagcta ccgcgacttc gcgtcgcgcg tcgtggcggg cgggatcttg
     1381 gcggatcctt ggatctctgg gctgccccgc ttccgcgaag agccgttgct ggtctccacg
     1441 gcccagcagc gcttgctcta tgacgccgcc gaagagatgg ccttcgtcta tgacgagctc
     1501 tgccgcatcg tctcggatga ccccgcgttg ctggacgatt tctttgggct gacgccctgt
     1561 cagaaggcga tgtggtttgc ttcgcagccg ctgtggcacg ggctcgcgag agccgatctg
     1621 ttcatgaccg ccgaagggcc cgtcatcgcc gagctcaact gcgatacccc aacgggtgaa
     1681 gccgaagcgg tggagctcaa tcgcctggtg cgcgaccaac atccgggaac gtacgacccg
     1741 aaccgggaac tcggtccacg gttttgcgcg cttctggata ccgtagcccg cgctgcgttc
     1801 gggcgtgcgc ccacgccgcc gctgcaagtc ggtttggttt accctaccga gttcacggag
     1861 gatctctcgc tggtgcggct gtatcgcggc tggctcgagg aaagaggtta caacgtgctc
     1921 ttgggttcgc cttacaacct cgagctcgag gggcgcggcc tctcgttgtt ctcggcgtcg
     1981 ttcaaattgc tccttcgcca ctacaagacc gattggtggg gcgaacgttc gagtgtgtgg
     2041 agcgacgaag acattcccga tccggaaccg ctcgcggagc cgctcgccgc catgctcacc
     2101 gcctccgcag aggggcaggt tgccatcatc aacccgctcg ccgccgtagt tccgcagaac
     2161 aagcgcgcca tggccttcat gtgggaacac ctccagcgct tctctccgca atcgcaggcc
     2221 gcaatccagc gctatctgcc tctgacttca cggcttgagg tcattcatcc cgagctcttg
     2281 atcgccgaac gtgaggactg ggtgctcaag agtgactacg gcgcagaagg tgaagaggtc
     2341 atcatcggac gccacacctc ggacgcgctc tttcgcgagt gtgtggccaa ggcgcggccc
     2401 ggccgttggg ttgcccaacg ctacttcgcc gcgctcgaga acgagcggcg cgagagcgtc
     2461 aactatggag tgtacttgat cgctggcgaa gccagtggtc tttacagtcg catccaggcg
     2521 ggcgccaccg acgtcaacgc gctgagcgta cccacattga tcgtgaagtg atcaacgccg
     2581 gcgagactgt tcgagcttcc gcgcgaagcc gcgctcgaac acgccgtcga cacggatgga
     2641 gtagcggaag aacgggtccg gctcgacacc gtgatagtcc atatcgttga accagtagat
     2701 tcgcgagtcc acgacacggc gctctcgatc ttccgggtcg caaacgtaga atcgcttgtt
     2761 cccgcggggt gagaacgaga tcgattgggc cacgcccagg ctcttccctg gctcactgtc
     2821 gcggtgagcc ggcgcgtggt ggtttgcttc gagtccgaac aggaccgcgc gcccgatctc
     2881 cacgaatggc aggctgcgca ggaattccac ggtcttcggg aagaattctc gggccggtgc
     2941 gtcgaactgc ttattcaaac cgctgtgttt gtcttcccag cgctggttct ctaggaaatg
     3001 atagcaaatc tgccagggga aatacacgcc ataccgatac ttcagataca gcatctgctc
     3061 gcgggtcagg gggtgatcgg tttcgtctcc gaatccatac tcacctcttc gctccgggtc
     3121 gaaatcctgc ggaacatggc cgagcgcgac gaactccaag aactcttcgc gggagaactg
     3181 cgagatgacc tggccatagt cgacgtacgg gtcacgctcc acccaaggcg cgcacacccc
     3241 catgtgcttc aggctgccgc ccgtaaacgt agtttccaca tggcacaagc ccacgctaat
     3301 ctcacgatcc agccgctcga agcacgagat gtcgatatga tccgtgaggt cgatataggg
     3361 ttctccccag atcccacaca agccggtcgt catgtttgca gatagctcct ggccaccatc
     3421 tgatcgaagg cgtgaacgcc gtcctcgacg agcgaataac aggcaggttc atagctgcga
     3481 gactgcacgc cccgctgctg gcgctcgcaa atggcgcggt cttctgcgtt gacctgatcc
     3541 cagaactcgt aaacatcccg ggcccacggc tctggcgcaa aggcggccat gtgaaagtat
     3601 gtggccgcaa ccacgcccgt gcgcgccggg ccgagcggct gcagacgata cgtcagcagg
     3661 taatccggct gcagactcgt gagcaacgtt ggaaacgcga aagcatccgc aactcggcgt
     3721 cgatcttcag gtggcacgat ccaagggcgt ttcggtgctg gctcggtaga ctctcccgcg
     3781 cttcgcggct ccgcggccac ggtgctcgcg tgcgaagcca gctgcatcaa gccgccgagc
     3841 catcccccac tccccttcca ggagcttgct ccggcgctcg gggtttggcg ctgcaagcca
     3901 ggatgaaccc gctcgaagtg gtgtgactcc tggaagttct cgacacagat tttccagttc
     3961 gcagcgactt catagctaac ggcacgcccg agccgaagca gatcgagctc cgagccctgc
     4021 agccactccg gggctcccgc gagagacgtc ccgagctcgg ccgtgtcgtt cccgaggcaa
     4081 acgaacacaa agccatgcaa cacgtcgact cgcacctcgc gtaagcctcg gcaggaggcc
     4141 gagaa
//
LOCUS       DASTKU010000603        14921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1903852, whole genome shotgun sequence.
ACCESSION   DASTKU010000603 DASTKU010000000
VERSION     DASTKU010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14921
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1903852"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFQ61_21045"
     CDS             complement(<1..318)
                     /locus_tag="VFQ61_21045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957002.1"
                     /translation="MPYSIWFIIIGALLIVMALSGTMLKRMPLSTSFLYLGVGLGLGH
                     HGLGILDLSPVRHSLLLERLSEVAVLISLFTAGLKLNAPFEDTRWALPFRLAFVSMAA
                     SVGL"
     gene            complement(476..1024)
                     /locus_tag="VFQ61_21050"
     CDS             complement(476..1024)
                     /locus_tag="VFQ61_21050"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9957003.1"
                     /translation="MDVIVIGDGPGGLSAALFLAKNGLKVSVYGQDKTAMHFASIRNY
                     LGIPAIHGSEFQKIAREQAQSFGAQLLDQRVESVSQDANGGFNVQLEDGKRATARYLV
                     LSEGKSPRLALQLGLAFDERTGLSVDRNGRTRIDRLYAVGRLTRPERSQAIISAGDGA
                     AVAIDILSSEQGKAVVDWESPA"
     gene            1329..3323
                     /locus_tag="VFQ61_21055"
     CDS             1329..3323
                     /locus_tag="VFQ61_21055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9957004.1"
                     /translation="MNVAEIAHRLSREFVVVCTTTGVCLSVDERAAATLGARVGEPLE
                     TCTDAGSRSKLAALLGRAQHGRVHNWELCMFSDQKPTTFSFEAEACEEGIVLVGTPVS
                     RAQSEAISAYQSALGDLSDLHRETSRQKGELHRLNQELSDSIQGLTSLHSELDEKNDT
                     LRRSADVKARLVANVSHEFRTPINSILGLSRLLLDRIDGELTEEQEVQVSFIRSNAET
                     LRNLIDDVLDLSKIEAGKISLRPRTFHIREMFAGLRGTMRALPRAEAVELIISDPTDV
                     PTMTSDDGKIAQILRNLVSNALKFTERGTVEVTAKAEPEGSISFAVTDTGIGVDPVHH
                     ETIFEDFGQIDSEAQRKVKGTGLGLSLSRRLASMLGGTLTVSSALGRGSTFTLTVPRV
                     HVEVAQMQALVERSKVLDPRRAPVLVLEDDRQTMFLYEKYLTQAGFQVIPARTTDEAR
                     AALGRVRPAAIVLDVMLETESSWRFLRDVKENPETHDIPVMVVTVVDSSQRARALGAD
                     EFWLKPIDPERIRRKLTELAKRGPVSVLLVDDDETSRYLVRKLLEDTPYVLHEASSWK
                     EAVQLASEKLPQVILLDFVLGDATAFDVIDDLKANPSTRGIPIIIQTAKTLDGVERER
                     LARDATAILPKQSLSRELAIARIREALEKSRLGTGDAHVS"
     gene            3313..4881
                     /locus_tag="VFQ61_21060"
     CDS             3313..4881
                     /locus_tag="VFQ61_21060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9957005.1"
                     /translation="MLASMPAPNLPSSIRVLNVNDDPSARYLATKMLRRAGFEVFEAD
                     TGMSAVRQARIHQPHLAVLDVRLPDISGLDVCRALKSDPDTASILVVQTSASFVTSAN
                     KAEGLSAGADAYLAQPFDAEELVATLHSLLRGRRQEAEARERANALTQADQRKDEFLA
                     MLAHELRNPLSAILTAARLLQTQDGEHSPKLAATIERQAHHLASLVDDLLDISRITHG
                     KIQLKMEPVDVLEAVRRAIDTVRSTFARHRHQLELHCCDGPLVVNADPVRLEQILCNL
                     LTNAAKYTNEQGHVEVNVDRVPDSDRVRIAIKDDGIGLSSEDAEHIFDLFYQVDTSIA
                     RSQSGLGIGLTMVKRLIEMHGGSVSVRSEGRGRGAEFNIELPEIAPPSKARTPSLAPR
                     ALHGTRVLFVDDNLDACELMQMSLQMAGCVVTTAHDGGEAVRLGTERRFDVAVLDIGL
                     PVLDGYQVAASLVETLGPLRPRLIALTGYNRAEDRGRAKRAGFDAHLVKPADSESLLR
                     TINDVLQGARVRLG"
     gene            4878..5519
                     /locus_tag="VFQ61_21065"
     CDS             4878..5519
                     /locus_tag="VFQ61_21065"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HET9957006.1"
                     /translation="MMSPLAERYLQAQLDGSRAEALAVVMEALHQGMTVPKIHLEIVQ
                     AAQYEIGRLWETNSISIAVEHQATAISQLALSNLYDRLPRAPTKGKRVLLACVEGELH
                     DMGSRIAADFLEMDGFEVRLLGANVPAVSLAERVEHYRPHLVALSATMTFHLPALESA
                     IAHIRASNQRNVPILVGGRAFSWGEAIAERLDVQGFGGDAEQLVREARRLTGS"
     gene            5560..6276
                     /locus_tag="VFQ61_21070"
     CDS             5560..6276
                     /locus_tag="VFQ61_21070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957007.1"
                     /translation="MQNAASDLATRLEQNIGPLSRRCTEAMYGDPFWSARFGARGRAH
                     AEADSGYHVKYVAAALRADDVTVFKKYALWLRGVLVSRNMCSWHLAESFRQLTLAMRA
                     EHLEGVDRAAEVMHEGALALGYQAGDAGELTAHLANIERQAQPESCYRIAELLSFLAD
                     GLAHSDDRTFACHVTFLNDTLARDASEHRALLDTLRTLREAALNLLSATAGSRVAKLI
                     DSCGLLEVPPPPTSNDAFAL"
     gene            6428..6931
                     /locus_tag="VFQ61_21075"
     CDS             6428..6931
                     /locus_tag="VFQ61_21075"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HET9957008.1"
                     /translation="MTSPKNGPAAKRLIQRRSKRSHRTTTPVQQTAPEEQIEPEERDA
                     HPPGSVMHDAFRRFAHRTSELVGSPWAFIGAVLVIAIWAATGPLFGFSDTWQLVINTG
                     TTIITFLMVFLIQNTQNRDARAIHLKLDELLRALENARDSMIDLEDLSDEELARLESE
                     FRHRFKR"
     gene            complement(6980..9115)
                     /locus_tag="VFQ61_21080"
     CDS             complement(6980..9115)
                     /locus_tag="VFQ61_21080"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9957009.1"
                     /translation="MRRTTEAVLALVQPRRSIWVRFGIGTGAALLGIAFRSALSVWLG
                     DGVPWITFFPMVLASAVLAGGVAATTTIALCSVLGTYLFATPVGAWPRDAQQWVSLLA
                     FVGNSSLVACLGVALRLSLARLEASRSEARRIADSLAERERELTLQVADLTQLHHFGT
                     AVVGMSSRDELLKMLLAAAVRLQSGERGFIELFDSITQRLALGVSFGHADSAIEALTS
                     VPVADGEGACGTCAARRTRVIVEDTEQDPLFTRYRDAARRAGFRAVWSTPLLTRRGEL
                     LGVLSTHFAEPKRPSEREVQLVELYARLAADAAEAAMIHARADEARLQAEAAAAQLRD
                     QDRRKDEFLAMLGHELRNPLAAIRAVVDIAQLRGRDSISGRELQVLDRQSTALARMLD
                     DLLDVARITHDKIDLQRTQVSVQAAVTRAVDSARAGIERRRHDLRIVLPPEPLFVEGD
                     PMRIEQVLVNLLGNAAKYTEPGGRIELHVTHEDDTIRIQVRDNGRGLAPGSENLIFDL
                     FQQDERKLDRAGGGLGLGLTIVKKLVELHGGRVFATSAGLGQGSQFTVELPELRVSSQ
                     LLDADTARADGLRDATSLRVLIVDDNVDAAASLAALLEFYGYSTRVAHDGPKAIELAR
                     EYEPQVVFLDIGLPEMDGYAVAASLRCMGAVARIVAVTGYGESEARARSLAVGISEHW
                     IKPVDGDEVLKFLAAFARDNRSSPLQAAV"
     gene            complement(9446..10405)
                     /locus_tag="VFQ61_21085"
     CDS             complement(9446..10405)
                     /locus_tag="VFQ61_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957010.1"
                     /translation="MRSPKAVWAMGLGAVATAVLVGIALRRGTGDSSSEHLQLQVSPS
                     LSATRETAPIGGSIGESGRNGSRANATEETTPAPRTLSEAIARYAEAFSNTSGTLDLG
                     SARLTLWAAQNGLDMSALRALPATSTLLFRRDPQAERGKLLCVSGVISDIRAERSLAR
                     RLVRDQSSPLVTRRSTAMGEGMPGAENQVWSGANAVEGNSGSRPISESPRTHDSTAEL
                     DPEGWRIPGGPVYFATITETVRSRNKPKPGPPLDNPLVVAAIAIGSAASLVDGDAAKF
                     CGVLTGVDQTDLPSGSLLRHRLIGVFEPTAPRGDLAAGPHAAE"
     gene            11081..11602
                     /locus_tag="VFQ61_21090"
     CDS             11081..11602
                     /locus_tag="VFQ61_21090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957011.1"
                     /translation="MRAVESLHGAAAAESCLSAMRAELAQGFRYQEILASGWYPIEWY
                     RDLLRAIQTALNLGERALFEIGRECARQDMTGIYKLGFKLLSPEVVLQLSTRLFSNYY
                     DTGKSKVVESRPGFAHVRWFDCAGFDKNMWWEAFGAAEMFMELAGARSVRTRIIRGGG
                     DTDEMAELTMHWT"
     gene            complement(11753..>14921)
                     /locus_tag="VFQ61_21095"
     CDS             complement(11753..>14921)
                     /locus_tag="VFQ61_21095"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HET9957012.1"
                     /translation="NGYLGALELTNVSGAPAKEYELLADVGGTKIGKCLLADCDAVEG
                     GYTFTSPRLLKLLPIRRGASYPILFMSRDPYAGITPYVISINGVRCDSVAPQVNLSAN
                     RTFFTANGTLHLTATASDNVAVRKVAFLRDGQEIGVDTDAPYEIDVPADSTQNGRHSY
                     TAVAYDPSGNQGTSNGVRVLTGIGNKFFGTAPDNAADYTHLLTYFNQLTPGNAGKWGS
                     VEATRNQMDWSKLDTAYNFAKQNGVRFKLHTLIWGQQQPDWLSSLTPQAQLAEIEEWM
                     SLAAQRYPDVDMVDVVNEPLHAPPAYSAALGGAGATGWDWVVKSFELARKYFPKAELL
                     MNDYNVEALEAWTTDYLKIINVLKERGLIDGIGLQAHFLERAELPVIAANLDRMAATG
                     LPIYVSELDVNFANDARHAQRLRDLFTLFWQHPSVLGVTHWGHLQGSMWRTDAYLIRS
                     DGTTRPGMDWINCFRVGNTNCTVPEYVPTRRSGDASGITLEAEDYDDARGILALGDVV
                     AYTDNTDWQSYDKVVFDKNWDSLSVTYARGGQDPANISVHLGSLDTPPVATVNLEPTG
                     GWGTNKTVSIPFAPISGEQNVFIRYNGGYGIANVDRFVFGAPANLGPNLIANGEFESS
                     ANGWFTWDGTLSSSTDYAVSGARSLKLGNRNGNGPAATSLAGLVVPGRKYEVALWTTV
                     GGSSSANVNITQKITCNGQDTYNWLINPTAVQPGQWTRLTGTLAVPDCNVTDVMIYAE
                     GPAGGIDMYLDHVSVRAQQVANIVTNGTFESGISGWSTYSGTASSSSARAHSGSKSLL
                     IGGRTGNAPAITNLTSAVTKGTSYQASFWVSIGGAASANVNLTRKIVCDGQTSYSWIA
                     NPVAVADGQWVELKGTLDIPNCTNLSEVLVFAEGPGAGVDLYVDDVNISVPSVTNLLS
                     EGSFESSASGWFTWGTGTLGLTQTRAHGGSKSLLLAGRTGNSPMARSLMGLIAPGSRY
                     QVSMWASIAGAATANVNVTRKFTCDGTDSYSWIINPVAVTDSGWVKLSGTLDVPNCAL
                     TDAIIYAEGPEAGVDLYLDDVVVSP"
BASE COUNT         2852 a         4681 c         4592 g         2796 t
ORIGIN      
        1 gaggcctacc gatgccgcca tggacacaaa tgccaggcga aacgggagcg cccaccgggt
       61 gtcctcgaac ggagcattga gtttcaggcc cgccgtgaac aacgagatga ggaccgccac
      121 ctcgctcagc cgctccagca acagcgaatg acgcacaggg ctgagatcca gaattcccag
      181 cccatgatga ccgagcccga gccccacgcc caaatacaga aacgaggtgc tgagcggcat
      241 ccgcttgagc atcgttcccg agagggccat cacgatcagg agggcgccga tgatgatgaa
      301 ccagatgctg taaggcatga ttgcggcggc cgcggagcac cgaatcgacg caaccgccac
      361 taagtggcgt ttttgcgccg atcacagggc tcctcgaact cgaaccgctc acacctgcaa
      421 cttacctgcc gtcgcgcggt gctcgaagag accgcgcgaa cggcgggggg tggactcagg
      481 ctggggattc ccaatccacg acggctttgc cctgctccga ggagaggata tcgatggcga
      541 cggcggcccc gtcgccggcg ctgatgatgg cctgactgcg ctcgggccgc gtcaaacggc
      601 ctacggcata cagccggtcg atccgcgtgc gcccattacg atctaccgag agcccggtcc
      661 tttcatcgaa tgcgaggccg agctggagcg cgagacgcgg tgacttcccc tccgaaagca
      721 ccagataacg cgccgtcgcc cgcttgccgt cctccaactg cacgttgaaa ccgccgttcg
      781 cgtcctgact gacggactct actcgctggt cgagcagctg agccccgaac gactgggctt
      841 gttcacgggc aatcttctgg aactccgagc cgtgaatagc cggaattccc aagtaattgc
      901 gtatcgaagc gaagtgcatc gccgtcttgt cttggccata cacgcttacc ttcaagccat
      961 ttttggccaa gaacaaggct gccgaaagcc cgcccggccc atcgccaatc acgatcacat
     1021 ccatatgttc ctccactcca cggtagcgat cgctcgctct cgttcgcgct gcaactagcg
     1081 accggcgtat gccccgaaag tggctcaaaa cacacgccaa tcggccagtc agcgctcaac
     1141 ctggaacaac tccggtgctg acttcaccgg aagtggctgc gtaaattcac ccaggctcct
     1201 ccacgccgaa cttccacccc atgagtgact attccaatcc gagaattgac cagaacgcca
     1261 cagtcttgcg atatgacgat gcactttgcc caatgagttc cacgcgctaa ctgctaaaaa
     1321 ctagcctgat gaacgtcgcc gaaattgcgc accgactcag tcgagaattc gtcgtggttt
     1381 gcacgacgac gggcgtctgt ctgtcggtgg atgaacgtgc ggcggcaacc ttgggcgcgc
     1441 gcgttggtga accactcgag acgtgtaccg atgccggcag tcgctcgaag ctcgcggctc
     1501 tgctcggcag agcccagcac ggccgagtcc acaactggga gctgtgcatg ttctcggatc
     1561 agaagccgac gacgttctcc ttcgaagccg aagcttgcga ggagggcatc gtgctcgtgg
     1621 gtacgccagt ctccagagcg cagtcggaag cgatctctgc ctatcaaagc gcgctcggcg
     1681 atctctccga tttgcatcgc gagacgtcac ggcagaaggg cgagctgcat cgcttgaacc
     1741 aagagctctc cgattcgatt cagggtctga ccagcctcca ctccgagctc gacgaaaaga
     1801 acgatacgtt gcgccggtcg gcggacgtga aggcccgctt ggtggccaac gtcagccacg
     1861 agtttcgcac gccgatcaac tcgattcttg gcctctcgcg gttgctgctc gaccgcatcg
     1921 atggtgagct caccgaggaa caggaggtgc aagtcagctt cattcgctcg aatgcggaga
     1981 cactgcggaa cctgatcgac gacgtcctgg atctctccaa gatcgaggcg ggcaagatct
     2041 cgctgcggcc gcgcacattc catatcaggg aaatgttcgc cgggttgcga ggcacgatgc
     2101 gggctttgcc gcgggccgaa gccgtcgagc tcatcatcag cgatcccacg gacgtgccga
     2161 cgatgaccag cgacgacgga aagatcgcac agattctgcg caacctggtg tctaatgcgt
     2221 tgaagttcac cgaacggggc accgtcgagg tgaccgccaa agccgagccc gaaggcagca
     2281 tctcgttcgc ggtcaccgat accgggatcg gcgtcgatcc ggtgcaccac gagaccatct
     2341 tcgaggattt cggtcagatc gacagcgaag cgcaacgcaa agtcaaaggc acggggctcg
     2401 gcctgtcact ctcccggcgg ctcgcgtcga tgctcggcgg caccttgacc gtctcgagcg
     2461 ccctcggacg cggctcgacc ttcactttga cggtgccccg agtccacgtc gaggtggctc
     2521 aaatgcaagc gctggtcgag cgcagcaagg tgctggatcc gcggcgcgcc cccgtgctcg
     2581 tactcgagga cgatcgacaa acgatgttct tgtacgagaa gtacctgacg caagctggat
     2641 tccaagtgat tccggcgcgc acgacggatg aagcgcgcgc cgcactcggg cgcgttcgtc
     2701 cggccgcgat cgtactcgat gtgatgctgg agacggagag tagctggcgg tttctgcgtg
     2761 acgtcaaaga gaaccccgag actcacgaca ttcctgtaat ggtcgtaacg gtggtggaca
     2821 gctcgcaacg agcgcgggcg ctcggggcag acgagttctg gttgaagccg atcgaccccg
     2881 agcgcattcg acgcaagcta accgagctcg ccaagcgagg ccccgtctcc gtgctgctgg
     2941 tggatgatga cgagacctca cgttatctcg tgcgcaagct gctcgaagat actccgtacg
     3001 tgttacacga agccagcagt tggaaggaag ccgtacaact ggcaagtgag aagctgccgc
     3061 aggtgattct gctcgatttc gttctgggag acgccacggc gttcgatgta atcgacgacc
     3121 tcaaggccaa cccctccacg cgcggaatcc ccatcatcat ccaaacggcc aagacgctgg
     3181 acggcgtaga acgagaacgt ttagcgcgcg acgccaccgc cattctgccc aagcagagct
     3241 tatcgcgaga actcgcgatc gccagaatcc gcgaagcact cgagaagtcg cgcctcggca
     3301 cgggagacgc ccatgttagc tagcatgccc gcccccaacc ttccttcgtc gattcgggtc
     3361 ttgaacgtga acgatgaccc gtcggcgcgt tatctggcca cgaagatgtt gcgccgcgcg
     3421 gggttcgagg tgttcgaggc cgacaccggg atgtccgccg ttcgccaggc gcgaattcat
     3481 cagccccatt tggcggtatt ggacgttcgc ttgcccgata tttcgggttt ggacgtgtgc
     3541 cgcgcactga agtcggatcc cgataccgct tcgatcttgg tggtgcaaac ctccgcatcc
     3601 ttcgtcacca gcgccaacaa ggccgagggc ctgagtgccg gagcggatgc gtacctcgcg
     3661 cagcccttcg acgccgagga gctggtggca acgctgcatt cgctattacg ggggcgtaga
     3721 caagaagcgg aagcgcgcga gcgagccaat gccctgaccc aagccgatca gcgcaaggac
     3781 gaattcttgg cgatgctcgc gcacgagctc cgcaatccct tgagcgcgat tctgacggca
     3841 gctcgcctcc tgcaaaccca agatggcgag cactcgccca aactggcggc aacgatcgag
     3901 cggcaggccc accacttggc gagcctagtc gatgatctat tggacatttc tcgcatcacc
     3961 cacggcaaga tccagctgaa gatggagccg gtggacgtgc tggaggcggt aaggcgtgcc
     4021 atcgacaccg tccgctcgac gttcgcgcgt caccggcatc aactcgaact gcactgctgc
     4081 gacgggccgc tggtcgtaaa cgccgacccg gttcggctcg agcagattct gtgcaacctc
     4141 ctcaccaatg ccgcgaaata cacgaacgag caaggccacg tggaggtgaa cgtggaccgc
     4201 gttcccgact ccgaccgtgt gcgaatcgcg atcaaggacg atggcattgg cctctcgtcc
     4261 gaggacgcgg agcacatctt cgacctgttt tatcaggtag acacgtccat cgcccgatcg
     4321 cagagtgggc ttggaatcgg cctcaccatg gtgaagcggc tgatcgagat gcacggcggt
     4381 tccgtcagcg tacgtagtga gggtcggggc cgcggcgcgg agttcaacat cgaattgccg
     4441 gagattgctc cgccgtccaa ggcgcgcacg ccctccctgg ccccacgagc cttgcacgga
     4501 actcgagtgt tgttcgtcga tgacaatctg gatgcctgtg agttgatgca gatgtctctg
     4561 cagatggcgg gctgcgtcgt tacgaccgca cacgatggtg gagaagcggt ccggctcggc
     4621 accgagcgcc gcttcgacgt ggcggttctg gatatcggct tgccggtgct cgacggctat
     4681 caagtggccg cgtcactggt cgagactttg ggcccgctac ggccccgatt gatcgccctg
     4741 accgggtaca atcgagccga agatagaggc cgcgcgaagc gggcgggatt cgacgcccac
     4801 ctggtcaagc cagccgactc ggagagccta ctcagaacca tcaacgacgt tttgcaaggt
     4861 gcccgcgtga ggcttggatg atgagcccac tcgcggagcg ctatctccaa gctcaactcg
     4921 acggcagtcg agcggaggcg ctcgcggtgg tcatggaggc tctgcatcag ggcatgacgg
     4981 tacccaagat tcacttggag atcgtccaag cggcacaata cgaaatcgga cgcctctggg
     5041 agacgaactc catcagcatc gccgtggaac accaagccac ggccatttct caactggcgc
     5101 tttccaatct ctacgaccgt ttgccccggg ccccgaccaa gggcaagcgc gtactcttgg
     5161 cttgcgtcga aggcgagctg cacgacatgg gctcgaggat cgcggccgac ttcttggaga
     5221 tggacggctt cgaagtgcgg ttgctcggcg ccaacgttcc cgccgtcagc ttggcggagc
     5281 gggtggagca ttaccgaccg cacttggtgg cgttgtcggc aaccatgacc ttccacttgc
     5341 cggcactgga gagcgcgatc gctcacatcc gcgcttcgaa ccagaggaat gttccaatct
     5401 tggtcggtgg cagggccttc agctggggcg aggccatcgc agaaagactg gacgtgcaag
     5461 gcttcggcgg cgacgcagag caactggtgc gcgaggctcg ccgattgact ggcagctaac
     5521 gtgaacggcg ccccgcacgc cgaagttgga gtacacccca tgcagaacgc cgcgagcgat
     5581 ctcgccactc gtttggaaca gaacatcggc ccgctatcac gacgttgcac cgaggccatg
     5641 tacggagacc cgttttggtc tgcgcgtttc ggagcgcgtg gccgcgctca cgcggaagcg
     5701 gatagcggct accacgtcaa gtacgtcgcc gcggcgctgc gcgccgacga tgtcacggtc
     5761 ttcaagaaat acgcgctctg gttacggggc gtcttggtgt cccgcaatat gtgctcgtgg
     5821 cacctggcgg agagctttcg ccagcttacc ttggccatgc gcgcggaaca cttggaaggc
     5881 gtcgatcgag cggcagaggt gatgcacgaa ggcgcactcg ccctcggata ccaggcgggc
     5941 gatgccggag agctaaccgc gcatctggcg aacatcgaga ggcaagctca acccgagagc
     6001 tgctaccgaa tcgcggagtt gctctcattt ctcgcggatg gcttggctca ctcggatgac
     6061 aggactttcg cgtgtcacgt cacgttcttg aatgacacgc tggctcgtga cgcgtcggag
     6121 caccgtgcct tactcgacac cttacgcaca cttcgagagg cggcgttgaa cctcctgagt
     6181 gcaaccgcag gcagtcgcgt cgccaagctc atcgattcct gtggattact cgaagtacca
     6241 ccgcctccaa cgtcgaatga cgcttttgcc ctctgaattg ccctaaccac gatttcacgg
     6301 gaatcccggg ctaagatgaa agcaatcttc atctctcgat gcagatgcga cgtgagcgcc
     6361 gaagcaacgc tgtcttggat tgagtgaggg cttggtcggc ggccacgcct gctgtactca
     6421 atactcgatg acctcgccga agaatggtcc tgccgcgaaa cgcctgattc aacgccggtc
     6481 gaagcggtcc catcgaacga ctacgcccgt gcagcagacg gcacccgaag agcagattga
     6541 acccgaagag cgcgacgctc atccccccgg gtccgtgatg cacgatgcgt tcaggcgctt
     6601 cgcgcaccgt acttccgagc tcgtgggttc accttgggcg ttcatcggcg cggtgttggt
     6661 gattgcgatc tgggccgcca cgggcccgct ctttggcttt tcggatacgt ggcaactcgt
     6721 cataaatacg ggcacgacca tcatcacgtt cttgatggta tttctgatcc agaacactca
     6781 gaatcgtgac gcgcgtgcga tccatctcaa attggacgaa ttactgagag cgctagagaa
     6841 cgcgcgagac agtatgatcg atctcgagga cctgagcgac gaggaactcg cgagactgga
     6901 gagcgagttc cggcaccgat tcaagcgttg acgactgggc acgttcgcgt aagcaccccg
     6961 tcgggcacac gaagatgact caaaccgcag cttgcagggg tgatgaccga ttgtctcgcg
     7021 cgaatgcggc tagaaacttc aggacttcgt cgccatccac cggcttgatc cagtgttcgc
     7081 tgatgcccac tgcaagcgag cgtgcacgcg cttcgctttc gccatatccc gtaacggcca
     7141 cgatgcgggc caccgcgccc atgcaacgca ggctggccgc cacggcatac ccgtccatct
     7201 ctggcaaccc aatgtccaaa aagaccacct gcggctcgta ttcgcgcgcc agctcgatgg
     7261 ctttgggacc gtcgtgcgcc acgcgcgtcg aatagccata gaactcgagc agggctgcca
     7321 ggctagcggc tgcgtccacg ttgtcatcga cgatgagcac gcgaagtgag gtcgcatctc
     7381 gcaagccgtc cgcgcgcgcc gtatcggcgt cgagcagttg agaactcacc cggagctctg
     7441 gcaattcgac ggtgaattgg ctaccttgac ccaagcctgc gctggttgcg aacacgcgac
     7501 cgccgtgcag ctcgaccagc ttttttacga tcgtgaggcc gagccctagc ccgcctccgg
     7561 ctcggtcgag cttgcgctcg tcttgctgga agagatcgaa aatcagattc tccgagccgg
     7621 gggccaagcc gcggccgttg tcacgtactt gaattcgaat cgtatcgtct tcatgcgtca
     7681 cgtggagctc tattcgcccc cccggctcgg tgtacttcgc ggcgttgccg agcaggttga
     7741 ccagcacttg ttcgatgcgc atcggatcac cttccacgaa caacggctcg ggcggcagca
     7801 cgatgcgcaa gtcgtggcgg cgcctctcga tgcctgcccg cgctgaatcc actgcgcgcg
     7861 tcaccgcggc ctgcaccgac acctgcgtgc gctgcaggtc gatcttgtcg tgcgtgattc
     7921 gggccacgtc cagcaggtca tccagcattc gggcgagtgc cgtactttgg cgatccagca
     7981 cctgcagctc gcgaccagaa atcgagtctc gcccccgcag ctgagcgatg tctacgaccg
     8041 cccggatcgc agccagcgga tttctgagct cgtgaccgag catcgcgaga aactcgtctt
     8101 tgcgccggtc ctgatcgcga agctgggccg cggccgcctc cgcttgtagg cgagcttcat
     8161 cggcccgggc atggatcatg gccgcttccg ccgcgtccgc cgcgagccgg gcgtagagct
     8221 cgaccagttg tacctcgcgc tcggagggcc gcttcggctc cgcaaagtgg gtcgacagca
     8281 cgcccaggag ttcgccgcgc cgagtgagca agggcgtact ccaaaccgct cgaaaccctg
     8341 cgcgccgcgc ggcgtcgcga tacctggtga acagcggatc ctgctcggtg tcttccacga
     8401 ttacgcgcgt tcgccgcgcc gcacaggtcc cgcaggcacc ctccccgtcc gccaccggca
     8461 cgctggtgag agcttcgatc gctgaatcgg cgtgtccgaa cgagacaccc agtgcgagtc
     8521 gctgcgtgat gctgtcgaac agctcaatga agccgcgttc gcccgactgc aaacgaacgg
     8581 cggcggcgag cagcatcttg agcagttcgt cgcggctgga catcccaacc acggccgtcc
     8641 cgaagtggtg gagctgcgta agatcggcga cctgcaacgt gagctctcgc tcacgctccg
     8701 ccaaagaatc ggcgatccta cgcgcttcgc tgcgcgaagc ctccagccgt gcaagcgaga
     8761 ggcgcagcgc cacgccgaga cacgctacga ggctggaatt gccaacgaac gcaagcagtg
     8821 aaacccactg ctgagcatcg cggggccaag cgcccaccgg tgtggcgaac aagtaagtgc
     8881 cgagcacgct gcagagcgcg atggtcgtgg tcgcagccac gccccccgcg agcacggcag
     8941 acgccaatac catcgggaag aaagtgatcc acggcacccc gtctcccaac caaacactga
     9001 gcgctgaccg gaatgcgatc cctaacaggg ccgcgccggt accgatcccg aatcgcaccc
     9061 agatcgatcg ccgaggctgc actagtgcga gcacggcctc cgtggttctc cgcatatatc
     9121 cacctatctc agccgacacc agcgcgcctc cagtatcggg agattaccat caatcgatac
     9181 ggcaaaatct agcgggcgtc gtcatacagc ctgaaggacg ccgaactacc tttcacgagt
     9241 ttcgtgaccg cctcgtcgct gaaactccct tgcaaaattc gcgattccgc cgtgacggat
     9301 ccgccggcgg gtttcgcacc aagatgcgac ggcctcgtta ctcgatgttt ccgcggtcct
     9361 tgttacgacg tgtttcgcac gctcggcggt ccgtggattc cctcagagcc agatgagctg
     9421 cgtccgcggc tctgcccccg tcgcttcact cggcggcgtg cgggcctgcc gccaagtcgc
     9481 ccctcggagc ggttggctcg aacacgccaa tcagtcgatg acggaggagg ctaccactgg
     9541 gcaaatcggt ctgatcgact cccgtcagaa cgccgcaaaa cttcgccgca tccccgtcaa
     9601 ccaacgaagc cgcgctaccg attgcgatcg cggccaccac cagcggattg tccagcgggg
     9661 gcccaggctt cggtttgtta cgggagcgaa cggtttcggt gatggtcgcg aagtaaaccg
     9721 gcccaccggg aatgcgccac ccttctgggt cgagctccgc ggtcgaatcg tgagtgcgtg
     9781 gagactcgga gattggacgc gagcccgagt tgccttccac cgcgttcgcc ccagaccaga
     9841 cttgattctc cgcgccgggc attccttcgc ccatggccgt gctcctccgt gtgacgagag
     9901 gcgaactctg atcgcggact aagcgccgcg ctaggctacg ttcggctctg atgtcgctga
     9961 tcactccgga gacgcataga agtttgccgc gctccgcttg aggatcgcgc cgaaatagca
    10021 acgtgctagt agccggcagc gctcgcaatg cgctcatatc cagtccgttc tgcgcggccc
    10081 acagagtgag cctcgccgac ccgaggtcga gtgtcccaga ggtgttgctg aaggcttccg
    10141 catagcgcgc gatagcctcg ctgagcgtgc gtggtgccgg cgtcgtctcc tcggtagcgt
    10201 tcgcacgaga gccgttcctt ccactctctc cgatcgaccc gccgatcggg gccgtctcac
    10261 gggttgcaga taacgagggc gagacctgta gctgaaggtg ctcgctactc gaatcacccg
    10321 tgccgcgcct caatgcaatg ccgaccagga ctgcggtcgc gaccgctccc agacccattg
    10381 cccagactgc cttgggactg cgcattttgg ctccaatgct gtgtagaacc gttcgcttga
    10441 acggttctgc tgcgtgaaat atcgaggacg cgcttttggc ccgcgtgctg accgcgcgcg
    10501 gttgcatgtt gccctctttg ggctccctga tcgagctcga aagaggctgc tccgcgcgac
    10561 ttcgaacgcc gcgtggtcac gcaccgcaaa atagtttcgg caccaagccc cgaccgcaag
    10621 ttgcttacgg cctgatcgcg cggtttgacg caagacaacg cttcgagggg tggtcaaaga
    10681 ggatcccgcg ttcgaaacca gtattgcacc ctagttgcat gtacaacctc gccgctcgtc
    10741 cgcgcggggc ggcagctttg gagccggcac acctatgggg gccggcgcgg tgtgtggggg
    10801 aaggttgggc gctgggtctg agccgaagcc gagcgtggat ggacttcgcg cacgtcaccg
    10861 cgtggccacg ctctagaatc ccgaattcca gcggagaatg tgatcaaagc tgggtcagtt
    10921 ctgccgattc ggacacccga gaccgctctt cgatcggtgc cgaacatgac gcacgctctg
    10981 cccgagcgtg gacgtgcgac cgcccatccg atacgcttga aaacgaggaa tggttgagcg
    11041 cggcgtagcc agagtgaagg gcatctcttt caggagtgtc atgcgcgcgg tggaatcgtt
    11101 gcacggggcg gccgccgcgg aatcctgtct ttctgcgatg cgtgcggagc tagcccaggg
    11161 cttccgatat caagagatcc tggcgtccgg ttggtatccg atcgagtggt atcgggatct
    11221 gctccgcgcc atccaaacgg cgctgaattt gggtgagcgt gcgctattcg agattggtcg
    11281 agagtgcgct cgccaagaca tgaccgggat ctacaagctc ggcttcaagc tgctctcccc
    11341 ggaggtagtc ttgcagctct cgacgcgcct gttctcgaac tactacgata ccggaaagag
    11401 taaagtcgtg gagtcccgcc cgggcttcgc gcacgtgcgc tggttcgact gtgcgggatt
    11461 cgacaagaac atgtggtggg aggccttcgg cgccgcggag atgttcatgg agctcgccgg
    11521 agcacgcagc gttcgtacac gcatcatccg tggcgggggc gacacggatg agatggccga
    11581 gctcaccatg cattggacct agagcatcgt ccgctcacag gccgaacgcg gcagaccaac
    11641 ccggcgatgg gcaggactac cgcgctcttt actcgcgtga tgagctcagt gccgaagagc
    11701 tcggacggcg agcggtgggt catgtctcga gtgacccacc gcctgtatcg catcaggggc
    11761 taacgacgac atcgtcgaga tagaggtcga cgcccgcctc cggaccttcg gcgtagatga
    11821 tcgcgtcggt cagggcgcaa ttggggacgt ccagggtacc actgagcttg acccagccac
    11881 tgtccgtcac tgccaccgga ttgataatcc aggagtatga gtccgtgcca tcgcaggtga
    11941 atttgcgcgt aacgttgacg ttggcagtgg ccgcacccgc gatcgatgcc cacatgctga
    12001 cctgataacg tgagccgggg gcgatcaggc ccatgagcga gcgagccata gggctgtttc
    12061 cggtacgccc cgcgagtagg aggctcttgc tgccgccgtg agcccgagtt tgcgtcaacc
    12121 cgagcgtgcc cgtgccccaa gtgaaccagc cgctggcgga cgattcgaac gatccttcac
    12181 tcagcaggtt ggtcacgctc ggaacactga tattcacgtc gtccacgtag agatcgacgc
    12241 ccgcgcccgg tccctcggca aagaccaaga cttcactcag attcgtgcag ttcggaatgt
    12301 cgagcgtgcc tttgagctcg acccactgcc cgtcggcgac cgcgactggg ttcgcaatcc
    12361 agctgtagct cgtttgcccg tcacacacga tcttgcgagt caggttgaca ttggcgctcg
    12421 ccgccccacc aatcgagacc cagaaactcg cttggtaact cgtgcccttg gtcaccgcgc
    12481 tggtcaggtt ggtgatcgcc ggcgcgttgc cggttcgacc tccgatcaag aggctcttgc
    12541 tgccgctgtg ggcgcgcgcc gagctggagc tcgccgtgcc gctgtacgtc gaccaaccgc
    12601 tgattcccga ttcgaaggtg ccgttcgtca cgatgttggc cacttgctgc gcgcggaccg
    12661 agacgtgatc caggtacatg tcgataccgc ccgcggggcc ctccgcgtag atcatcacat
    12721 cggtgacatt gcagtccgga acagcgagtg tgcccgtgag gcgcgtccat tggccgggct
    12781 gaacggcggt tgggttgatg agccagttat acgtgtcttg accattacag gtgatcttct
    12841 gggtgatgtt gacgttggcg ctggagctac caccgacggt ggtccacagg gctacttcgt
    12901 atttgcgacc gggcaccact agccccgcga ggctcgtcgc ggcgggtccg ttgccgttgc
    12961 gattgccgag cttgaggctg cgcgccccgc taacggcgta gtccgtgctc gaggagagcg
    13021 taccgtccca tgtgaaccaa ccgttggcgc tgctctcgaa ttcgccgttc gcgatcagat
    13081 tcgggcccag gttggcaggt gcgccgaaca cgaaacgatc gacattcgcg attccgtaac
    13141 cgccgttgta gcgaatgaat acgttctgtt ctccgctgat cggggcgaac gggatggaga
    13201 cagtcttgtt ggtgccccaa ccgccggtcg gctctaggtt taccgtcgcg accggcggcg
    13261 tatccaagct gccgaggtgc acgctgatgt tcgcggggtc ctgcccaccg cgcgcgtacg
    13321 tcactgacag gctatcccag ttcttgtcga agacgacctt gtcgtagctc tgccaatccg
    13381 tgttgtcggt gtatgcaacg acgtcgccca aggccaggat gccgcgggcg tcgtcatagt
    13441 cctcggcctc cagggtgatt cccgaagcgt cgcccgaacg ccgcgtcggt acgtactccg
    13501 gaacagtaca gttggtgttg cccactcgga aacagttgat ccagtccatg cctgggcggg
    13561 tcgtgccgtc cgagcgaatc aagtaggcgt ccgtgcgcca catgctgcct tggaggtggc
    13621 cccagtgggt aactccgagt acggacgggt gctgccagaa tagcgtgaac aggtcgcgca
    13681 agcgttgggc gtggcgcgcg tcattggcga agttgacgtc gagctcggag acgtagattg
    13741 gtagtccggt cgccgccatc cggtcgaggt ttgccgcgat caccggcagc tcagcgcgct
    13801 ctaggaagtg cgcctggagg ccaatcccgt cgatcaggcc gcgctctttg aggacgttga
    13861 tgatcttgag gtagtccgtc gtccaggcct ccagcgcctc gacgttgtag tcgttcatca
    13921 agagctcggc cttcgggaag tacttgcgag cgagctcgaa cgacttcacg acccagtccc
    13981 agcccgtcgc acccgcaccc ccgagcgccg cgctataggc aggcggcgcg tgcagcggct
    14041 cgttgacgac atcgaccatg tcgacgtcgg gataacgttg ggcggccaga gacatccact
    14101 cttcaatctc ggcgagttgc gcttggggcg tgaggctgct caaccaatca ggctgctgtt
    14161 gaccccaaat caatgtatgg agtttgaagc gaaccccgtt ttgcttggca aagttgtagg
    14221 cggtgtcgag cttcgaccag tccatctggt tgcgcgtcgc ttcgacactg ccccactttc
    14281 cggcgttacc gggtgtgagc tggttgaagt aggtcaacag gtgggtgtag tcagcggcat
    14341 tgtcgggagc ggtaccaaag aacttgttgc cgatcccggt gaggacccgc acgccgttgc
    14401 tcgtgccctg attccccgag ggatcgtagg ccacggctgt gtagctatgt cggccatttt
    14461 gggtcgagtc ggccgggacg tcgatctcgt agggtgcgtc ggtgtcgacg ccgatctctt
    14521 gaccgtcgcg caagaacgcc accttgcgga cggcgacgtt gtcgctcgcg gtggcggtca
    14581 aatgcagggt accgttcgcg gtgaagaacg ttcgattggc gctgaggttc acctgcggag
    14641 ccacggagtc acagcgtacg ccattgatcg agatcacgta gggcgtgatt cctgcgtagg
    14701 ggtcgcggct catgaacaga atgggatagc tcgcgccgcg gcgaatcggc agcagcttga
    14761 gcagccgagg agaggtgaag gtgtagccgc cttccacggc atcgcagtcg gcgaggaggc
    14821 actttccgat cttggtaccc ccgacgtcgg ccagaagctc gtattccttt gcgggcgcac
    14881 ccgaaacgtt ggtaagctcc aacgccccga ggtacccatt a
//
LOCUS       DASTKU010000604         7984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_340486, whole genome shotgun sequence.
ACCESSION   DASTKU010000604 DASTKU010000000
VERSION     DASTKU010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340486"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VFQ61_21100"
     CDS             <1..997
                     /locus_tag="VFQ61_21100"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9957013.1"
                     /translation="SDTPELVQKVTTQWGPRADAWVKGLLHRSGPPEPAEPPIPTPSL
                     QVHREPDGAYSIEIGSGIDVVQRGPGHWRIGPAGSHPASGVDVSRLTAQGLEEFVSYI
                     KHNAFELIRFGHTVVRAVSRSIFLLFMTLMVAGYLMHTREAILGFFRSLVPPRARISF
                     DHLLFRIDRGLAGVVRGQLLICAVNGVLSLIGFWMFDLKYWPILTIVAAVFSIVPIFG
                     SILSSVPAVLIGLSQGFWTALWILLWIIGIHQIEANLLNPKIIGVAAKIHPVLVVLAL
                     LTGEHYFGLWGALLAVPALSVAQSIFNHFRFEALPDAPPDSLLPPEPNVVRGRAT"
     gene            1138..2127
                     /locus_tag="VFQ61_21105"
     CDS             1138..2127
                     /locus_tag="VFQ61_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957014.1"
                     /translation="MANETRKIRRAKERETGNKPPRPDLSKPLSLKDLAESNPSSAGG
                     PGQVPDPFGKEQLKSILVRLGIPLLAAWMLCGFVASVVYSQTWKIVLIAIPALLTALA
                     AGVLVWVFRQAKKARGVASILRNVENAEDRKAALSQLETSYKKSDPAAVFARAQLEMQ
                     EDPKKALATLEQIDLSKVMAPVADEARAQRAMIHLMQGDVSLARPLADGIDLTRHQDA
                     RSRAMMAAVCSEAWARSGAAKKALETLGLFDPDEATFEQLRPQLYRAWAFAYAQTADT
                     KGMRRVLKKLATQDVRLLMGFLVKRTHPLLQKEARKMIEQSGLLPRRMNIQRN"
     gene            complement(2190..3314)
                     /locus_tag="VFQ61_21110"
     CDS             complement(2190..3314)
                     /locus_tag="VFQ61_21110"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HET9957015.1"
                     /translation="MKERAASFMARVDAPGRAARALAGVLADVRHPSGALIFASGSLS
                     ARLVELGRELKAVLGDTPAMLAVGHGVLTERGEIEHESAIAGIVWSGGTTECVGVPSS
                     EGDLGYSLARSLDGHAQRAATAFAFVRAKGFLPHMLEPLREQRFACMVGAGTAGEVSV
                     VPIAPGLPLEPAAAGALILKNLTPPTVKASPGCRLLSKMRRITRVRGGMVLELDGYRA
                     LEALSEAANGLTDQLLFVALAEHLSADDPPALLLRGIQGVDPLQHGVLVSDEVRAGQF
                     MAFAVRNAEQSRIELEQRVARLARDAGGARPHFGVYVSCAGRGRTLYGSEDVDTRIIR
                     ARFPQLPLAGMHSSFEIAPHGGGPALQLYTGVLTVFTEPS"
     gene            3437..4252
                     /locus_tag="VFQ61_21115"
     CDS             3437..4252
                     /locus_tag="VFQ61_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET9957016.1"
                     /translation="MPSQHELKMCSYGGTDIGRRRAINEDSYLADDELGLWVVADGMG
                     GHAAGEVASRESVDVIFGMVRRGKLSLNLGGEPSEEKARAACRVLEGAVQAATYLVFA
                     MAELDRGKAGMGTTISAMMRFGNSVVLAQVGDSRIYRVRERKAQQLTEDHTLIAWQLR
                     QGLITEDEARVSKQRNVITRAVGSRDYVQVDTSVWESNPGDAFLLCSDGLHGYVNLEE
                     IGHALTQGGERAVRSLIGLANSRGGKDNITAVVVEVSADAESITSPPDAESAS"
     gene            4421..5443
                     /locus_tag="VFQ61_21120"
     CDS             4421..5443
                     /locus_tag="VFQ61_21120"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET9957017.1"
                     /translation="MAHLEEEPAPLRMMRLLFGGLISQMLALTVQLNLPDRLKHGPLT
                     AGFLAQQLNLDPVATYRLMRGLSSLGVLQEHMHETFSLTVLGETLRRDLPGSLAGLAR
                     LAGEKWHWSALAELPHAVATGESAFSRAHGTGMFQWLEQHPEASEAFSDAMSSFSAIE
                     AHLVASAYDFTRVTRVADVGGGHGLLLAEILSRYSNVEGVLFDLPHVASGAESTFAER
                     GLSHRSRVIAGDFFEAVPGDCDVYLFKHILHDWDDIRARRILSRVAQAMPSDARVLIA
                     EQVLPPIGVAHFSKIMDVGVLVMTEGGRERTQAEHAALLESAGLVLVEVTPTPGPLSL
                     VEARRA"
     gene            complement(5518..7299)
                     /locus_tag="VFQ61_21125"
     CDS             complement(5518..7299)
                     /locus_tag="VFQ61_21125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9957018.1"
                     /translation="MTERPSEAETTLSAALAELARVRGELKQAQDEIQRLRRPAYPAP
                     ARDESSTAAVTMTINDSPELLARAAKEAACVRIAADSSPCGIMCISGPEGRYVYINQA
                     FANLVGRSLEATLEADPYHIFLEVTHPEDVAIERVQLDRLGRGEIDSYELEKRFMSPG
                     GKSRRVLAKVSATRDAQGRLDNLIANFTDVHDYRDAQQAREELTAQLRQSQKLEVLGR
                     LAGGVAHDFNNRLVIIMGYTELLARRLEPENPLLEHTQTVLESAQRAAELTRQLLAYS
                     RRQVLKPQVFDLNVTVDRMRQLLERLIGEDVELVTVLGAKNPIVSDPGQIEHVILNLA
                     VNARDAMPDGGRLTLETRDAVIEKPDGRTSHLAAGEYVVLTVTDTGIGIPEAVVPHVF
                     EPFFTTKEPGRGTGLGLSTAEGIVHQSGGAISVESQPGRGTRFLIHLPRAHTEAVRAA
                     PPESTAPKSGVSFETVMVCDDDDGVRQLLAQVLLLRGYQILQARDGQHALEVARQHRS
                     RIHLLVTDLIMPGLSGIQLAERMRALDPELRVLFISGYTEDHRELSDLLGQTTHFLPK
                     PFLPGELTSAVCALLEQRAHAPARANP"
     gene            complement(7709..>7984)
                     /locus_tag="VFQ61_21130"
     CDS             complement(7709..>7984)
                     /locus_tag="VFQ61_21130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9957019.1"
                     /translation="RNIFNALRSGGVYGIVDHSARAGSGLSDAQTNHRIEQEALVSEI
                     QAAGFRLDAQTDVLRNPADTRDWNGSPREAGERRGTTDRFVLRFVKP"
BASE COUNT         1439 a         2453 c         2560 g         1532 t
ORIGIN      
        1 gagcgatacg cccgagctcg ttcagaaggt gaccactcag tgggggcctc gcgcagacgc
       61 ttgggtaaaa gggctgctcc atcggagcgg gccgcctgaa cctgcagagc cgccgattcc
      121 cacgccatcg ctgcaggtgc accgtgaacc cgacggtgct tacagtatcg agattgggag
      181 cggcatcgac gtcgtgcagc gcgggccagg tcactggcgt atcggtcccg cgggcagtca
      241 tcccgcttcg ggcgtagacg tgagccgtct cacggcgcag gggctcgaag agttcgtcag
      301 ctacatcaaa cacaacgcct tcgagctgat tcgcttcggg cacacggtgg tccgagccgt
      361 cagccgtagc atcttcttgc tcttcatgac gctgatggtc gccgggtatc tcatgcatac
      421 tcgcgaggcg atcctcggct tctttcgttc actcgtgccg ccgcgggcgc gcattagctt
      481 cgaccatctg ctgttccgca tcgatcgagg tcttgccggc gtggtgcgcg ggcagttgtt
      541 gatctgcgcc gtcaacggtg tgttgtcgtt gatcgggttc tggatgttcg atctgaagta
      601 ctggccgatc ctgacgatcg tcgccgcggt gttcagcatc gtgccgatct tcggatcgat
      661 cttgagctcg gtcccggccg tgctgattgg cctcagccag ggattttgga cggcgctttg
      721 gatcctgctt tggattatcg gaattcatca gatcgaggcc aacctgctca atccgaagat
      781 catcggcgtg gcagccaaga tccatcccgt gctggtggtt ctggcgctgt tgacgggtga
      841 acactacttc ggcttgtggg gagcactgct tgcggtcccc gctctctcgg tggcgcaaag
      901 catcttcaat cacttccgct tcgaggcgtt gcccgatgcg cctccggaca gcctgcttcc
      961 gccggagccg aacgtcgttc gtggtcgcgc gacctagttc ccgcgcgagc tccaacgcga
     1021 ccatcaccgg ggcgacgtcg gcgaaccact ccgagcgcga gcgtaactcg gcgaagaccg
     1081 tgcagctttg gtgggagtcg gttgagcggc tgaggcgaac gcgctatacg acacgccgtg
     1141 gccaacgaaa cgcgcaagat ccggcgcgcc aaagagcgtg agacaggcaa caagccgccg
     1201 cgtcccgatc tgagcaagcc tttatcgctg aaggatctcg cggagtcgaa tccatcgagc
     1261 gcgggtgggc cagggcaggt tccggaccca tttgggaagg aacaactcaa gagcatcctc
     1321 gtgcgtctcg ggattccgtt gctcgcggcc tggatgttgt gcggattcgt ggcgagcgtg
     1381 gtgtactcgc agacctggaa gatcgtgctg atcgcgatcc ccgcgctgct cactgcgctc
     1441 gcggcaggcg tattggtttg ggtgttccgg caggccaaga aggcgcgggg ggtggcgagc
     1501 atcctgcgta acgtcgagaa tgccgaagac cgtaaggctg cgttgtcgca gctcgagacc
     1561 tcttacaaga agagcgatcc tgcggcggtg tttgctcggg cccagctcga aatgcaggag
     1621 gacccgaaga aagccctcgc tactctggag caaatcgatc tctcgaaggt gatggctccg
     1681 gtcgctgacg aggctcgtgc ccagcgcgcg atgatccatc tgatgcaggg cgatgtcagc
     1741 ttggcgcgcc cgctcgccga tggcatcgac ctcacgcgac accaggacgc ccggtcgcgc
     1801 gccatgatgg ccgccgtctg ttcggaagct tgggcgcgga gtggtgcggc caagaaggcc
     1861 ttggagacgc tcggtttatt cgaccccgat gaggcgacgt tcgaacagct gcggccccag
     1921 ctgtatcgcg cttgggcgtt cgcctacgcg cagacagccg acacgaaggg gatgcggcgc
     1981 gtgctgaaga agctcgccac ccaggacgta aggctgctca tgggctttct ggtcaagcga
     2041 actcatccgc tactgcagaa ggaagctcgc aagatgatcg agcagagcgg gttgctgcct
     2101 cgccggatga atattcaacg caattgagtc gacggagagc ggcgtgcaca cggggcgctc
     2161 gcccctttcc gctcggcgag ttccgtcggt cagcttggct cggtaaacac cgtgagcacg
     2221 cccgtataga gctgcagtgc gggtccgccg ccgtgcggcg caatttcgaa cgaagagtgc
     2281 atacccgcca acggcagctg tggaaaccga gctcggatga tgcgcgtgtc cacgtcttcg
     2341 gagccgtata gcgtccgccc tcgcccggcg cagctcacat aaactccgaa gtgcggccgg
     2401 gctcctcccg catcccgcgc cagccgcgcc acgcgttgtt cgagctcgat gcgggattgc
     2461 tcagcgttgc gcacggcgaa cgccatgaat tgacccgcgc gcacctcgtc cgaaaccagc
     2521 accccgtgtt gcaacggatc gactccttga atgccacgca agagaagagc cggtggatcg
     2581 tccgcactga gatgttccgc gagcgccacg aataggagct ggtcagtaag cccgttggcc
     2641 gcctcggaca gcgcttcgag cgcgcggtat ccatcgagct ccagcaccat cccgccgcga
     2701 acgcgcgtga tgcggcgcat tttggaaagc aggcggcagc cgggggaggc tttgacggtg
     2761 ggtggcgtga ggttcttgag tattagggcg ccggctgccg ccggctcgag cggcagcccg
     2821 ggcgcgatcg gcacgacgct gacttctccc gcggtgcccg ccccgaccat gcatgcaaaa
     2881 cgttgctcgc gcagcggttc gagcatgtga gggagaaagc ccttggcgcg tacgaaagcg
     2941 aacgcagtgg cggcgcgctg cgcgtggccg tccagcgaac gtgcgagcga gtaacccaaa
     3001 tcaccctcgg acgagggcac acccacgcac tcggtcgtac ctccgctcca cacgatgcct
     3061 gcgatcgcgc tttcgtgctc gatctcaccc cgctcggtaa gaacaccatg acctacagcg
     3121 agcatggctg gagtgtcccc cagtactgcc ttcagctcgc gacccaattc cacgaggcgg
     3181 gcgcttagcg agccgctcgc gaagatcaat gcgccggatg ggtggcgcac atcggccaaa
     3241 acacccgcta gcgctcgtgc agcgcggcca ggggcgtcga ctcgcgccat gaagctcgcc
     3301 gcacgttcct tcattcgcgt cgaaagatag gccggattgg gtcccgctgc gttcggaaaa
     3361 agcttcccag aatgcccgcc ccatgacggt ggggcccttc accggcccgc tgaactcccg
     3421 tatcctctgg acgaaaatgc cgtcccagca tgagctcaag atgtgctcct acgggggtac
     3481 cgatatcgga cggagacgag ccatcaacga ggactcctac ctggcagacg atgagctggg
     3541 cctttgggtc gttgcagatg gaatgggggg tcacgccgca ggagaggtgg caagtcgcga
     3601 aagcgtcgat gtcatcttcg gaatggtgcg acgcgggaag ctctccctga atttgggggg
     3661 agagccttcg gaggagaagg cgcgcgctgc ttgtcgcgtt ttggaggggg cggtgcaggc
     3721 ggccacctac ctggtttttg cgatggcgga gctcgaccgc ggtaaggccg ggatgggcac
     3781 gaccatcagc gccatgatgc ggtttggaaa ctcggtggtc ttggcgcaag tgggcgacag
     3841 tcgcatctac cgcgtgcgcg agcgcaaggc tcaacagctg accgaggatc ataccttgat
     3901 cgcgtggcag ctccggcagg gcctgatcac ggaggacgag gctcgcgtca gcaagcaacg
     3961 caacgtgatt acgcgggccg ttggcagtcg tgattacgtg caagtagaca ccagcgtttg
     4021 ggagtcgaac cccggggacg cattcttgct ttgcagcgat ggtctgcacg gttacgtcaa
     4081 cctcgaagaa atcggtcacg ccctgaccca aggcggcgag cgcgccgttc gctcactgat
     4141 cggcctcgcg aactcacgcg gtggcaagga caacatcacc gccgtagtcg tcgaggtgag
     4201 tgcggacgcc gaatcgatca cgagcccacc cgacgcagaa tccgcaagct agcccgcgag
     4261 ctcactgcgc ccaaaaccca gctcagcaaa cccagctcag caaacccagc tcagcaaacc
     4321 ctgcaaattg ccccgaggca aagcgtgcct ggacctctgg cattgaccgg gcggagcttt
     4381 cgagtgacta ttctcggctt cgacaacccc gaggttattg atggcacatc tggaggagga
     4441 accagcgccg ttgcgcatga tgcggctgct gtttggtggg ctaatctcac aaatgctggc
     4501 gctcacggtc cagttgaact tgccggatcg gctgaagcac ggaccgctca cggccgggtt
     4561 tctggcgcag caattgaact tggaccctgt ggcgacctat cgattgatgc gcggtttgag
     4621 ttcgctcggt gtgcttcagg agcacatgca tgaaaccttt tccctgactg tgctggggga
     4681 gaccttacgc agagatctgc cgggctcctt ggccggcttg gctcgcttgg ccggcgagaa
     4741 atggcattgg tcggctctcg cggagctgcc gcacgccgtc gctacgggtg agagcgcgtt
     4801 ctcgagagct catggaacag ggatgttcca atggctcgag cagcaccccg aagcctccga
     4861 agctttcagc gacgcgatgt cgagcttcag cgcgatcgag gcccatctcg tagcgtccgc
     4921 gtacgatttc acgcgcgtca cgcgcgtagc ggacgtgggg ggcggtcacg ggctactgct
     4981 tgcagagatc ttgagccgct attcgaacgt cgagggtgtg ctcttcgatt tgcctcacgt
     5041 ggcaagtgga gccgagtcta cgtttgcgga acgaggacta tcgcaccgta gtcgcgtcat
     5101 cgccggagac tttttcgaag ccgttccagg tgactgtgac gtctaccttt tcaaacacat
     5161 cttgcatgac tgggatgata ttcgggcgcg ccgaattctg agtcgcgtcg cgcaggccat
     5221 gcccagtgat gcgcgtgtgc tgattgcaga acaagtgctg cctcccatcg gcgtggcgca
     5281 cttctccaag atcatggatg tcggcgtgct ggtcatgacc gagggcggtc gcgaacgtac
     5341 ccaggcggag cacgctgcgc tgttggagag cgcgggctta gtgctggtgg aggtcacgcc
     5401 cacaccaggg ccactcagtc tggtcgaagc gcgccgagct tgaaagcgaa gctcgggatg
     5461 ggcccggagg gcgctcgtga gagtcaggtt tcttattgga acccgctggg actctcatca
     5521 gggattggcg cgcgcgggag cgtgcgcccg ctgctccaat aaggcgcata ccgccgaggt
     5581 tagctcccct ggcaagaacg gcttcggcaa aaagtgagtg gtctggccga ggagatccga
     5641 gagctcacgg tgatcctcgg tatacccaga aatgaagagg acgcgcagct cgggatccaa
     5701 ggcgcgcatg cgctccgcca attgaatccc gctcaagccg ggcataatca gatccgtgac
     5761 cagcaagtga atgcgactgc gatgttggcg cgcgacttcg agcgcgtgct ggccatcgcg
     5821 cgcctgtagg atctggtagc cgcggagtaa caagacttgc gccaacaact ggcgcactcc
     5881 gtcgtcgtcg tcacaaacca tcaccgtttc gaagctgacg ccgctcttcg gggcggtgga
     5941 ttccggcggg gcggcccgca cggcctcggt atgcgcgcgc gggaggtgga tgaggaatcg
     6001 agtgccgcgg cccggttggc tctcgaccga aatcgcgccg ccactctggt gcacgatgcc
     6061 ttcggccgtg gagagcccta gcccggtgcc gcggcctggc tctttggtcg tgaagaatgg
     6121 ttcgaagaca tggggcacta ccgcctccgg aatcccgatt ccggtgtctg tgaccgtaag
     6181 caccacgtat tctccggccg ccaggtggga ggtccgtcca tcgggctttt cgatcaccgc
     6241 atcccgagtc tcgagcgtga gccgcccgcc gtcgggcatg gcatcacgag cgttcacagc
     6301 caaattcaga atcacgtgct cgatctgacc aggatcggac acgatgggat ttttggctcc
     6361 gagcacggtc acgagctcga cgtcttcccc gatcagtcgt tccagcaatt ggcgcattcg
     6421 gtcgaccgta acgttcaggt cgaagacctg cggcttgagc acctgccggc gactgtaagc
     6481 caacaactgt cgggtcaact cagcggcgcg ttgtgcgctc tccagcaccg tttgcgtgtg
     6541 ctcgagcagc gggttttcgg gctccagccg tcgtgccagg agctccgtgt aacccatgat
     6601 gatgaccaag cgattgttga agtcgtgcgc aacgcccccc gccagacggc caagcacctc
     6661 tagcttctgc gattggcgaa gctgcgccgt caactcctcc cgcgcttgct gggcgtcgcg
     6721 gtagtcgtga acatccgtga agttggcaat caggttatcc aggcggcctt gtgcgtcgcg
     6781 agttgcgctg accttcgcga gcacgcgcct cgatttcccc cccggcgaca tgaagcgctt
     6841 ctcgagttcg taggagtcga tttctccgcg gcccaaacga tcgagctgca ctcgctcgat
     6901 cgccacgtcc tcggggtgcg tgacttccag aaaaatgtgg tagggatccg cttccagcgt
     6961 agcttcgagg cttcgcccta cgaggttcgc gaaggcctga ttgatgtaga cgtagcgacc
     7021 ttcggggccg gagatgcaca tgatgccgca gggcgaggaa tcggcggcaa ttcgcacgca
     7081 ggcggcctct ttggccgcac gggccagcaa ctccggtgag tcattgatgg tcatcgtgac
     7141 cgcagccgtc gacgactcgt ctcgcgccgg agcgggatac gctgggcggc gcagtcgttg
     7201 aatttcgtct tgagcctgct tcaactctcc acgcacgcgg gccagttcgg ccagcgcagc
     7261 cgagagggta gtctccgcct cagaagggcg ctccgtcatt caaaccgggt tataccaggt
     7321 ccgtagcgtc ccggagtacc cgcgttgctc cgttgttggc aaagcatgcg atcggttcat
     7381 gcttacgggg tttaccgaca acacgaagct ggccgccgcc agggattgcc cgcgaaggag
     7441 gccctttcga agagggcagc tcccgagtgc tagcgtttca gttcgtcttg gcgttaccgt
     7501 ttggtgtgaa gtcgacggag cggttcggat caccgccgct cattcacttg ctacccaagc
     7561 gacggactaa ggtcggttcc gtaacggact ggttttccga cccgctcagg ccctgaccgg
     7621 ttctttccgc ctattccctt ctcgtgcgaa ggggcgtgac acaatgcccc gctcggctcc
     7681 aaaccggccg tttcgctgtc ccgaatgctc acggcttcac gaaccgcagc acaaagcggt
     7741 cggtcgtgcc ccgtcgttcg cccgcctcgc gcggagagcc gttccagtcg cgcgtatcgg
     7801 ctgggttccg cagaacgtcc gtctgcgcat ccagtcgaaa gccagccgct tggatctcgc
     7861 tgaccaaggc ttcctgttcg atgcgatggt tggtttgcgc gtcgctcaaa ccgcttccgg
     7921 cgcgggcgct gtgatccaca atcccgtaca ccccgcccga tcgcagcgcg ttgaagatgt
     7981 tccg
//
LOCUS       DASTKU010000605        13274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_340814, whole genome shotgun sequence.
ACCESSION   DASTKU010000605 DASTKU010000000
VERSION     DASTKU010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13274
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340814"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1264)
                     /locus_tag="VFQ61_21135"
     CDS             complement(164..1264)
                     /locus_tag="VFQ61_21135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET9957020.1"
                     /translation="MPKRAHDVRLTWPGKASGFAGSNAGARLEPDLPESSRSNAVLIE
                     GDNITALRALQGLPARATLVYLDPPFFTQREHERVERARGPRGVERRSFKAFDDRWAN
                     FEQYLSELGQRIAAARELLAPEGCLVLHVDPKTSHYAKVLCDEIFGVEAFASEIVWRY
                     RRWPAKTRNFQRVHDVLLRYVRDPEATPRFRQLYEPLAASTEKTWGAGRQRAVVNADG
                     RRYRSSVTDEPSPGVPLGDVWDISIIAPVARERTGYPTQKPEALIERLIEACTDPLDL
                     VIDAYAGSGTTLAVSTRLGRRAIGIDQNPEAIALTRKRLGAQGVDAFEHRVVAGPSIA
                     QIKPERVAKTPRTATAGAPERPVGSRRVRRVA"
     gene            complement(1469..1975)
                     /locus_tag="VFQ61_21140"
     CDS             complement(1469..1975)
                     /locus_tag="VFQ61_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET9957021.1"
                     /translation="MQSSQEVEFKVGDKAVYPAQGVAEVISIDEKDIAGQRQRFYVLR
                     ILDTDRKIMVPVSNANAVGLRQVISEQEIREIFDILKERTIGFDTQTWNRRYRGFMDK
                     IKTGSIYDVAEVLRDLYRLKANKQLSFGERRMLDTARGLIVKEIAIARAQTEEDVKNE
                     IEAIFFSN"
     gene            complement(3517..4470)
                     /locus_tag="VFQ61_21145"
     CDS             complement(3517..4470)
                     /locus_tag="VFQ61_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET9957022.1"
                     /translation="MTSESSRRLAAIDIGTNSVLLLVAEATRGRIEVLVERAEITRLG
                     EGVDRTGRLSEAACERTLAVLRAYAAECRSLGVERLHAVGTSAMRDAAEGREFVDAAE
                     GILGARPEVISGRREAELTFEGALSGLSLEGPVQVFDIGGGSTEIILGTAARAGTAAE
                     SIESAVSLDIGSVRLFERCIRTEPASSEDLARVRQEIRRALAMAPAPAFGGTLVGVAG
                     TVTTLLALELGLVEYDARRVHGGVMSHDALSSLASRLEKLNLEQRRAIPGLAPGRADV
                     IVAGAAIAVEVMAWSRAREMIVSDRGVRWGILQRMARGQAV"
     gene            complement(4467..5267)
                     /locus_tag="VFQ61_21150"
     CDS             complement(4467..5267)
                     /locus_tag="VFQ61_21150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9957023.1"
                     /translation="MRRIVPLAVLFPLLAACGYSQEEWDQKLRENEGLRTELANERQA
                     HKKTQADYAAANQEIDNLKKQLTERGISLDSLNASLAEQRKALEEYARRTEQLDAIKK
                     RFELLSSKLQKLTQLGLKVEVRNNRMLIQLPGDVLFDSGRDKLKPDGEKILLQVAEVI
                     RTDNDLSRRRFQVAGHTDAKPLQGGQFKDNWGLSAMRARSVAVLLTTPVDKGGGGLSV
                     ANWSAAGYAESDPVSTNETEEGRQKNRRVELVVQPDVTEMLNLQSLTK"
     gene            5661..5810
                     /locus_tag="VFQ61_21155"
     CDS             5661..5810
                     /locus_tag="VFQ61_21155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957024.1"
                     /translation="MAAKKTRTKSARTPAAKKAPKVAKKRATKRRVKKAGKKRAKKAA
                     KKAAE"
     gene            complement(6246..6797)
                     /locus_tag="VFQ61_21160"
     CDS             complement(6246..6797)
                     /locus_tag="VFQ61_21160"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9957025.1"
                     /translation="MALSSHRSQFGCWLGSLGASVIMAATGCGGSAAPTRTTADIPAA
                     SEPPDPEPARSAEAQKPKEDEKWEGEDEATKAASGTGASGAEARTNEVIAKVIRDNRK
                     PFRDCYEKAAKEQPGIEGTLTLHFVLTPDGKVKSAELNRERSTLTAPTAVDCAVSALK
                     TLKFPPSSRGMESVVNYPFDFKR"
     gene            complement(6869..7609)
                     /locus_tag="VFQ61_21165"
     CDS             complement(6869..7609)
                     /locus_tag="VFQ61_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET9957026.1"
                     /translation="MDLARAGPGRAFFRAAAFGAFPSEPWPPRRSVTLNPSYAPGVER
                     LVREQEPQTHAGRDVLTLPVLLLNRHFAPVSVATVRRAFVLLYGGAARAVDGEGETYD
                     FGDWLNMPVRHSDDGLPIIGGQVRAPRVLHLLRYDRAPRIVVRLTRKNLMLRDQFQCQ
                     YCGRRPHVRDLNVDHVLPRSRGGEDSWENLVVSCRACNLRKGRRTPTEAGMALLSVPH
                     RPRWSTATQIRLVMREPFAEWQPFLETG"
     gene            7793..9436
                     /locus_tag="VFQ61_21170"
     CDS             7793..9436
                     /locus_tag="VFQ61_21170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957027.1"
                     /translation="MFLNLLSIRHASSNLLNTEHHAAARASRSSRWLICAVALAGLWI
                     SGCGGALGQGEAEWPPLAKRWYDRAQVSFRAGDIEDAELAIENALKVVPKREEARLLA
                     AQIALAELKYDRAVAVLEGLETSAAHAIRGRAYWYAGEVERAADELEKLLADPAVRDP
                     WATEISKLARLGSGRKPFSVSGMLAATDMPKAGTAAMIVPLELNGEPALGLVATGSPE
                     AAIDSSGGARSSWVSLRFGERFQVQDVPALAQDLSGLSRQVNAPIKILLGANLLRHLH
                     ATFDFTGSQFVVRSFDPPPPPAATTVKLSYVRGGGMLLRGTFGNDQASPIGALLLDTT
                     YNFPLALTDRGWKKAGVDTKILQPSRVGGGVKEGLLPMLRIGAFDLPQVPSRQGEVAL
                     KEREETLGGLELDGLIGSGLLATFRGTLVDGGRTLWLEDLPAEALNPPPLMAPPSDGL
                     EGLGSEDLEAAPEEEAPATKPGARPPAGKSPPPGAGRPATPGGAAPGRAPAPAPAAAP
                     PAPKAAPNAGTPSSAAPATTPGASGGGAVRGGAPSGARP"
     gene            9433..9924
                     /gene="coaD"
                     /locus_tag="VFQ61_21175"
     CDS             9433..9924
                     /gene="coaD"
                     /locus_tag="VFQ61_21175"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET9957028.1"
                     /translation="MSDEAKLAIYAGSFDPPTYGHVDVVERAAKLFPRVLVAIGRNPQ
                     RNPLFNVDERVDLMRSVCSGFDNVSVDSFDGLLVDYGKRVGARVIVRGLRAGTDFEYE
                     LQIAHANADLRPELDTVFLPTRANYGFISASLVREIASYGGDVSRYAPPPVCAALKKK
                     FVG"
     gene            10039..11703
                     /locus_tag="VFQ61_21180"
     CDS             10039..11703
                     /locus_tag="VFQ61_21180"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9957029.1"
                     /translation="MNQTRNQTSELKIVIVGGVAGGMSAATRARRVNEHASIIVLERS
                     GYISFANCGLPYYLSGKIKNQADLFLTTPARVGSRFRIDARVHHEVTRVDRTRRCVEG
                     INRLTQESFVVPYDKLILAPGAKPLLPPLENLEAGNVFVLRNIEDTLRLDAFLRERQP
                     KRAVVVGAGFIGLEMVEALCDRGLEVTLIEKAPHVLPALDAQMAGWLERELRTHGVRL
                     ELGTGVTELLGNREGVHGVRTEAGALLETDLVLLSIGVRPTVELAERSGLALGRSGGI
                     QVDRALRTSDPDVYAVGDATEVTQGVSHFATRVPLAGAANRQGRIAGEHAASGQAPPS
                     PQVLGTAIVRVFGLEVGVTGLGRAAAAQAGYSVGTALVHPKHHAGYYPGAQQMHFELV
                     YDEATGKVLGAQAAGGAGVDKRLDVIATLLHFGGTLEDLAALDLAYAPQFSSAKDPVH
                     MAAFVAQNDRQRLCPSTDEVPRGVQLLDVRTRPEFERGALPNAVNIPLDELRERLGEL
                     DPHEPVVVYCEVGIRGYAATRILLQRGFRHVQNLKGGYLQQSRTAS"
     gene            complement(11723..13078)
                     /locus_tag="VFQ61_21185"
     CDS             complement(11723..13078)
                     /locus_tag="VFQ61_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957030.1"
                     /translation="MMKRTPTAEFAVPKPDPTDPEPVRRALLSAEASHAASDLAATAN
                     WLERAAELALQSQLQARAASLSESARDVTKLLAQADSPPRKAQVDAIFDELFGDPLLP
                     GRGGEVQRSRAAVTVSTENVDAESSAPGRRSVPTPPPLPEEKAPFGAAPAPAQRTEDE
                     TVEHQTDSQSHRASPESMVAPIPVPVAVEPAALSAPVAQAVPPAAMSPVLVPEILTRE
                     SETRMQDKQVDAPRLSQLDIEWEDATAYVDADEEEESQVPEAVVEAHPEVIPPPLPLA
                     AVQVTPEPPPREPPPPLPMPPVESNAAAEALQRAVSISAKAPPKPPSVGRASNQPGST
                     SPLPQSSSAEPPSFAGPPSFAGPPSFAEPPSSANHSNQDVAPPNVAPEVGATQPNVGT
                     SLDDRFAMAQCPALRVSLIPTQDEARFILRVLGEGEQPAPGADEALLIGSGAGLLLSN
                     S"
BASE COUNT         2385 a         4249 c         4150 g         2490 t
ORIGIN      
        1 tgcggccttc ttgatgtcct gagccagagc caagcttgcg accagaggga caaccagcgc
       61 gggccagaaa ccgagacgga gcgtacgaga ggtcattggc gagggagctg gttttaccca
      121 attccgagtc gctaaggaac cttgctcgct cgaacacgcg gattcatgcg acccgacgca
      181 cgcgccgcga gccgactggt ctctccggcg cgcccgcggt cgccgttcta ggcgtcttcg
      241 ccacccgctc tggcttgatc tgcgcgatgc ttggacccgc caccacgcgg tgctcgaacg
      301 cgtccacccc ttgtgccccc aaacgctttc gcgtcagcgc gatggcctcg gggttctgat
      361 cgatgccgat cgcacgacgc cccaaacgcg tgctgacggc gagcgtggtc ccactacccg
      421 cgtaggcatc gatcaccaaa tccagcgggt ccgtgcaggc ctcgatcaag cgctcgatca
      481 gtgcctcggg cttctgtgtc gggtagcccg ttcgctcccg agctaccggc gcgatgatcg
      541 agatgtccca gacgtctccg aggggcaccc caggagaagg ctcgtccgtc acgctcgatc
      601 ggtaacggcg cccgtcggcg ttgactacgg cgcgttgcct accggcaccc caagttttct
      661 ccgtggaggc cgcgagtggc tcgtacaact ggcggaaacg cggagtggcc tcgggatcac
      721 ggacatagcg cagcaacacg tcgtgcacgc gctggaagtt gcgcgtcttc gctggccaac
      781 gccggtaacg ccacacgatc tcgcttgcaa acgcctccac cccgaaaatc tcgtcgcata
      841 ggaccttggc gtagtggctc gttttcggat ccacgtgcag cacgaggcat ccttcgggcg
      901 caagcagctc acgtgcggcc gcgatgcgct gccccaactc tgagagatac tgttcgaagt
      961 tcgcccagcg gtcgtcgaac gccttgaagg acctgcgctc tacgccgcgc ggcccgcgcg
     1021 ctcgctctac acgttcgtgt tcgcgttggg tgaagaaggg cgggtcgagg tagaccaacg
     1081 ttgcgcgtgc tggcaatcct tgcagcgccc ttagcgcggt aatattgtca ccctcgatca
     1141 ggactgcgtt gctccggctg ctctccggca agtccggctc gagccgagcc cctgcgttgc
     1201 ttcccgcaaa gccagatgct tttccaggcc aagtgaggcg aacgtcgtgg gcgcgcttgg
     1261 gcatcgccgc gaagctaatg actttgcgcg attgggccta attttggagg tcgaaaccgg
     1321 aagcacacga gctcgagggc cgttcggatc caagcctcta cgcccacgcc tcttcgcgat
     1381 cgcccacggc gttaccgagc tcctcgcagg agctcgaaat ggaagaaggg cgttcaccca
     1441 cggaagtgag caaacgccct ctaccaactc agttcgagaa gaagatcgct tcgatctcgt
     1501 tcttcacgtc ttcttcggtc tgagcgcgag caatcgcgat ttctttcacg atcaggccac
     1561 gcgccgtatc cagcatgcgg cgctcgccga acgagagctg cttgttggcc ttgaggcggt
     1621 acagatcacg cagcacctcg gctacatcgt aaatcgatcc ggtcttgatc ttgtccatga
     1681 aaccgcggta acggcgattc caggtctgcg tatcgaaccc gatcgtccgc tccttcagga
     1741 tgtcgaagat ctcgcggatt tcttgctcac tgataacttg acgcaaaccg acggcgttgg
     1801 cattcgacac aggaaccatg atcttgcggt cggtatcgag gatgcgcagg acgtagaaac
     1861 gctggcgttg gccggcgatg tccttctcgt cgatgctgat cacttcggcc accccctgag
     1921 cggggtacac agccttgtca ccgaccttga actcaacttc ctggctcgat tgcatgacgt
     1981 ctccttgact gcgcggtcgt tcaatccgtc gtgtgagcac tgctcacgca acgccgccga
     2041 acgaccatcg aatcgaatgc ggcttgaatc ccgtttgttc ttcgcggcgg cagattgagg
     2101 gccgacagta acgaacggga tatgagtttc attccagcct ttagcgaaac cggttcccgc
     2161 aaggttgcgt aacgatttcc acagcaagcg ggggctacta ttaaccgggt ctgataatcg
     2221 gggattggct cgacctgctt gtcggtttgg actcgatcga cttagagcga ctctaaccga
     2281 tatctgttgg gccgatattt gttgcgccga ctctgcagcg ctgatgtctg ttgcgccgac
     2341 gatgttgctc gaacgtgttt gccgaagcga aattgccggt gcgagcggcc ggtgaccagt
     2401 cccggtgcat gcgtggcccg cgcagaaatg atgcgtattg gccgtgcggg agtagctttg
     2461 ctgccttagt gcgctgctgc ttgctggcgc ggctgctttc agctgcgctg tgccttgacg
     2521 acctgacgtc gcctcgacga cctgatgtgc cataacggcc ttggcctgcc taatggcccc
     2581 gaaggaactt tgccttcggg ctcgctaacc tcaggtaccc cgcgaccagg caccttgcgt
     2641 tcgtcatccg agcgtccatc acctcgacga ttctggagcc cttgggggag gcccactgaa
     2701 ttttgtggtc aggtttcgcc aggcgcggca ctctagtcgg cttctacttg tctgtcaact
     2761 attcgtgcca attcgccgaa aacttgcgct aagaactccc tgagtttcga accagtgcgg
     2821 acgctcgccg ccgtaacgag actaccaaca acttctccac cggctctcac caacaagcct
     2881 gacccgctag ataccgacat gaccggcgag tggtcctagg ctgacctatg acgcagatag
     2941 tcaactcgag tgcgtgagct cgaccgagct ctgcccacgt ggagcggtgg cccggtagag
     3001 atgcgtttgc ggcatatccg cgaggctgtg agctgacctg tcgtgcctgt cgacctgtgc
     3061 cggcgttatc gactcacctt gctgcacctg tccgagcttg gctgctcggt gcaaacaacc
     3121 gtcgctgaca accacgaacc aacccggagc caaaacggcc ctcaggtaag cagtagtatg
     3181 acctgttcgc acacaaagtc ccgttccctc gagcggtcgg cacagctttc ttgaggaaac
     3241 atgactttcc cgggcgttcg cgggcttcgc ggaaatgggg aatctcccat ttcttgcggg
     3301 caggccatcg tggctgggcg tttcttttgc gggtaagagc agcgtacgcg aacgaggcag
     3361 ctcagttcga tactgggaga cccactcgag actcaacctt cactcaaatt gagccggcgt
     3421 gctggagcac gcgacgctcg aggccagggg acacgctccg ccaggcacgt ctggagagca
     3481 cagcgtgcct tggggcggaa tgagccagat gctgaactaa actgcctgac ctcgagccat
     3541 tcgctgcagg attccccagc gcaccccgcg gtcggagaca atcatttctc gggctcggct
     3601 ccaggccatg acctcaacag caatggctgc ccccgccacg atcacgtctg ccctgccggg
     3661 tgcaaggccc ggaatcgcac gccgctgctc caggttcagc ttctccagcc tgctggcgag
     3721 ggagctcaag gcgtcgtggc tcatcactcc gccgtggaca cgtctagcat cgtattcaac
     3781 gagcccgagc tcgagggcca gcagagtcgt cacggtgccc gcgacgccca ccaatgtgcc
     3841 gccaaacgcc ggcgcagggg ccatggccag ggctcttcga atctcctggc gaacccgcgc
     3901 gaggtcttcg ctgctcgcgg gctcagtccg gatgcagcgt tcgaagagcc gcacgctgcc
     3961 gatatccagg ctaacggcac tctcgatgga ctccgcggcg gttccggctc tcgcagcggt
     4021 gccgagaatg atctcggtgc ttcctcctcc gatatcgaag acctgaactg gcccttccag
     4081 cgaaaggcca gaaagcgccc cctcgaaggt gagctcggcc tcgcgtctcc cggaaattac
     4141 ctcgggacgg gcccccaaga tcccctcggc ggcgtcgacg aactcacgac cctcggcggc
     4201 gtcgcgcatt gcgctggtac cgacggcgtg caggcgttcg acgcctaagc tgcggcactc
     4261 cgccgcatat gcccgtaaga ccgcgagagt gcgctcacaa gccgcttccg acaaccgccc
     4321 agtacgatcc acaccttcac ccaagcgggt gatctcggcg cgctcgacca aaacctcgat
     4381 gcggccccga gtagcttccg ctaccaagag cagcaccgag ttggtcccga tatcgatcgc
     4441 cgctagacgc cgcgacgatt cggacgtcac ttcgtcaagc tctgaaggtt gagcatctcc
     4501 gtcacgtccg gctgcaccac gagctcgaca cggcggttct tttggcgccc ttcctcggtt
     4561 tcattggtcg aaactgggtc actctcggcg tagccggcgg cgctccaatt cgctactgaa
     4621 agcccgcctc cacctttgtc cacgggagtg gtgaggagga ccgcgaccga gcgcgcgcgc
     4681 atcgcactga gcccccagtt atccttgaac tgcccgcctt gcagcggctt cgcgtccgtg
     4741 tgtccggcaa cctggaaccg ccgacgcgag agatcgttgt cggttcgaat gacctcggcg
     4801 acctgcagca ggatcttctc gccatcgggc ttcagcttgt cccgcccgga gtcgaacagc
     4861 acgtcacccg gcagctgaat cagcatgcgg ttgttacgaa cttcgacttt gagtcccagt
     4921 tgggtgagct tctgaagctt gctcgacagc agttcgaagc gctttttgat ggcgtcgagc
     4981 tgctccgttc ggcgagcgta ctcttcgagt gctttgcgtt gttcggcaag gctcgcgttc
     5041 aagctgtcga ggctgatgcc gcgctcggtc agctgcttct tcaggttgtc gatctcctga
     5101 ttggcggccg cgtaatccgc ctgcgtcttc ttgtgcgcct ggcgctcgtt cgcgagctcg
     5161 gtccgaaggc cctcgttctc tctcagcttt tgatcccact cctcctggga gtagccgcac
     5221 gcggccaaga gaggaaagag aaccgctagg gggacaatgc gtcgcatgaa catcggtatc
     5281 tcgcgcgccg ggttgaaagt ttttcggcgc tccaagcaca ggcgctatct cagtcggacc
     5341 cggcgcgtca agtgttggag ccgcttcact tttctggcgc acggccgaag ctgtgcacag
     5401 cggccgagcc gcttttattg ggctcccgcg ggcactgaac aggctcgccc tccgcaatcg
     5461 ctgcggtccg caggatgcct gaattcagct cggccccgcg atcccatgca tttttttcgt
     5521 tgacacatca cactaggtgg caatatacgt cgtgcaacat accctcggga tctcaagatc
     5581 ggcacgaggc cccctgctgg agccccgttc gctgatggcg gattccgaca ccggattgat
     5641 tccgagacag ggaggatcga atggctgcta agaaaacccg tacgaagtcc gctcgtacgc
     5701 ccgctgccaa gaaggccccg aaagtcgcca agaagcgcgc caccaagcgt cgcgtgaaga
     5761 aggcgggcaa gaagcgcgca aagaaagctg cgaagaaagc agcggagtga tgcgcacggt
     5821 cgatcggcac taacttcgct cgccgctcga atccacacac acgctgccct cggatgcgag
     5881 ggagctcgga cgagctaagc gagagaagtc atgcacggca aagctgcaaa gcttccttaa
     5941 cgagggcggc aaagccgccc aaagggctca aaagtccgag ttagggccgt gaagagccga
     6001 tcatattgag tgctcgcctg gtgcgagccg cccgaagcca aacgcttcgg caccgggcac
     6061 gcgaatgaga actccggggt tttcgttcgc gtgctcggca caagaccgtc ttcgctgtgc
     6121 gcctgacacg gcgttccgaa gcggcaatgg ctccgagcca gcaatggctg agccatctcg
     6181 aacgaacgag ttcgaatcgc tgagtcgaac tcccgagttc cttcgtgcgg tcgagtcgag
     6241 gcgactcagc gtttgaagtc gaatgggtag ttgaccacgc tttccatgcc gcgcgaggaa
     6301 ggcgggaact tcagcgtttt caaggcggaa actgcgcagt ccaccgcggt cggcgccgtg
     6361 agggtcgagc gttcacggtt gagttcggcc gacttcactt taccgtcggg cgtgagcacg
     6421 aagtgcaggg tcaacgtgcc ctcgatgcct ggctgctcct tggctgcttt ttcgtaacag
     6481 tctcggaagg gcttgcggtt gtcgcggatg accttggcaa tgacctcgtt cgtacgcgcc
     6541 tcagcgccgg aggcgccggt gcccgatgcc gctttcgttg cctcgtcctc gccctcccac
     6601 ttttcgtctt ctttgggctt ctgagcctct gcgcttcggg cgggctccgg atcgggaggc
     6661 tcggacgcgg ccggaatatc cgccgtcgta cgggttggag ccgcgctgcc cccgcatccg
     6721 gtcgcggcca taatcacaga agccccaagg gaccctagcc agcaaccaaa ctgcgagcga
     6781 tgactcgaaa gcgccatgag cgcagcatat ccgcctgccc cgggctgtgg gcaaactctt
     6841 gactattggc cgccggcagc cgggcgactc agccggtttc tagaaaaggc tgccactccg
     6901 caaaaggctc ccgcattacg agccgaattt gagtggccgt ggaccatcgc gggcgatgtg
     6961 ggacagaaag cagcgccata cccgcctccg tcggcgttcg acgccccttc cggagattgc
     7021 acgcccgaca cgagacgacg aggttctccc aggagtcctc accgccgcgc gagcgcggca
     7081 aaacgtggtc cacattcaag tctcggacgt gtggacgacg gccgcagtac tggcattgga
     7141 actgatcgcg cagcatcagg ttcttgcgcg ttagacgcac cacgatccgc ggcgcgcggt
     7201 cataacgcag cagatgcagc accctgggcg ctcgcacctg cccgccaatg attggcaacc
     7261 cgtcgtcgga atggcgtact ggcatgttca gccagtcacc aaagtcatag gtttctccct
     7321 cgccgtctac tgcacgagcc gctccaccgt agagcaacac gaaggcacga cgcaccgtgg
     7381 ctacactgac gggagcgaag tgccgattga ggagcaatac gggaagcgtc aggacatcgc
     7441 ggccggcgtg ggtttgaggc tcctgttctc taaccaaacg ctccacgcct ggagcatagc
     7501 ttggatttag ggttacggac ctgcgcggcg gccagggttc ggacgggaac gcgccaaacg
     7561 cggccgctcg aaaaaaggct ctgcccggtc cggcacgtgc gagatccatc caggacggaa
     7621 tactctccgc cctctcctga tgaccgtcgc tgttggtttc agaaagccga ggcgttcaac
     7681 ccgcaagcgg ctctcgaccg cgtgagctcg atacgctaat gccgttcgcc tctcctaccg
     7741 cttcgcactc gtgcgccctt ggctcacacg ctcactgact gtgtcggtcc cgatgtttct
     7801 gaacttactc tctatccgcc acgcctcttc taacctgctg aacaccgagc atcatgctgc
     7861 cgcgcgcgcg agccggagct cacgttggtt gatctgcgcc gtagcgctcg cgggactctg
     7921 gatttccggc tgcggcggcg cgttgggtca gggcgaggcg gagtggccgc ccctcgccaa
     7981 gcgctggtac gaccgcgcac aggtcagctt ccgcgcgggc gatatcgaag acgcggagct
     8041 cgcaatagag aacgctctga aggtcgtgcc caaacgcgag gaagcgcggc tactcgctgc
     8101 ccaaattgcg ctcgcggagt tgaagtatga ccgcgccgtg gccgtgctcg aggggctgga
     8161 gacgagcgcc gctcacgcga tccgcggccg cgcctactgg tacgcgggcg aagtcgagcg
     8221 cgcggcggac gagctcgaga agttgctcgc ggatcctgcg gtgcgggatc catgggccac
     8281 cgagatctcc aagttggcgc gactgggtag cggaaggaag cccttcagcg tgagcggcat
     8341 gctggccgcg acggatatgc cgaaggccgg cacggcggcg atgatcgtcc cgctcgagct
     8401 aaacggagaa cccgcgctag ggctcgtagc caccgggagc ccggaagcag ccatcgattc
     8461 gagcggggga gctcgctcgt cctgggtctc gctgcgtttt ggcgagcgct tccaggtcca
     8521 agatgttccc gcgttggccc aggacctctc cgggttgtca cgccaggtga acgcgcccat
     8581 caagattttg ctgggtgcga acctactgcg tcacctgcat gccacgttcg acttcacggg
     8641 cagccagttc gtcgttcgct cgttcgatcc gcctccgcct ccggcagcga cgacggtcaa
     8701 gctgagctac gtgcgcggcg gaggcatgct gctgcgcggt accttcggca acgatcaggc
     8761 ctctcccatc ggggccttgc tgctcgacac gacttacaac ttcccgctcg ccctcacgga
     8821 ccgaggttgg aagaaggccg gggtcgacac gaagattctg caaccttctc gcgtcggggg
     8881 aggcgtcaaa gaaggcctac ttcccatgct gcgaatcggt gcgttcgatt tgccgcaagt
     8941 gccgagtcgt caaggcgaag tggccctgaa ggagcgagaa gaaacgctgg gcgggctcga
     9001 gctggacggg ctgatcggct ccggattgct cgccacgttt cgaggcacgc tggtggatgg
     9061 cggccgcacg ctgtggctcg aagatctgcc ggcggaagcc ctcaatccgc cgccgctgat
     9121 ggctccccca agcgacggtt tggaagggct cggcagcgag gatctagaag cggctcccga
     9181 ggaagaagcg cccgcgacca agcccggcgc tcgaccgcct gccgggaagt cgccaccgcc
     9241 gggagcaggc agacccgcca ccccgggtgg cgccgctccc ggtcgagcgc cggcgcctgc
     9301 gccggccgct gcaccgcccg cgccaaaggc agccccgaat gcgggaaccc cgagcagcgc
     9361 tgctccggcg accactccgg gagcttcggg cggaggtgca gtacgtggag gtgcaccgag
     9421 cggagctcgc ccatgagtga cgaagccaaa ctggcgatct acgccggaag cttcgaccca
     9481 ccgacgtacg gccatgtcga cgtcgttgaa cgagccgcga agttgttccc gcgcgtgctc
     9541 gtcgcaatcg ggcgcaatcc gcagcgcaat ccgctgttca acgtcgacga gcgcgtggac
     9601 ctgatgcgca gtgtgtgctc ggggttcgac aacgtcagcg tggacagctt cgacggcctg
     9661 ctcgtcgatt acggtaaacg cgtgggggcg cgggtgattg tgcgtggcct ccgcgcgggc
     9721 accgacttcg agtacgagct ccagatcgca cacgccaacg ccgatttgcg ccctgagttg
     9781 gatacggttt ttcttccgac tcgcgccaat tacggcttca tctccgcatc cctggtgcgg
     9841 gagattgcga gttacggcgg cgatgtgagt cgctatgcgc cgccgccggt ttgtgctgcg
     9901 ttgaagaaaa aattcgtcgg ctgacaaaaa gcatctcgcg ccgtaccggc cgccggtatc
     9961 tcgactgtcg aagcagtctg agccgcgaac atctcgtacg ttctgcatgc tcgaggccta
    10021 tccgcccccg gagcgtgcat gaaccaaaca agaaatcaga cttctgaact caaaatcgtg
    10081 atcgtaggtg gcgtggcggg aggcatgagc gcggctaccc gcgcgaggcg cgtcaacgaa
    10141 cacgcaagca tcatcgtgct cgaacgcagc ggctacatct cgttcgcgaa ctgtggcctg
    10201 ccctattact tgagcgggaa gatcaagaac caggcggatc tgttcttgac cacgcccgcg
    10261 cgcgtgggaa gccgatttcg aatcgatgct cgggtacatc acgaagtgac gcgggtcgat
    10321 cgaacgcgta ggtgcgtaga gggcatcaac cgcctcactc aagagtcatt cgtcgttccg
    10381 tacgacaaac tgatcttggc gcccggagcg aagccgcttc tgccgccgct cgagaatctg
    10441 gaggccggca acgtattcgt gctgcgcaac atcgaggaca cgctacggct cgatgcattc
    10501 ttgcgtgagc gccagccgaa acgcgccgtg gtggtgggcg cggggttcat cggactcgaa
    10561 atggtggagg cgttgtgcga tcgcgggctc gaggtaacgc tgatcgagaa ggcaccccac
    10621 gtcctccccg cactcgacgc ccagatggcg ggctggctcg agcgcgagct gcggacgcac
    10681 ggcgtaaggc tcgaactcgg gacgggcgtg accgaactct tgggcaaccg cgaaggtgtg
    10741 cacggcgttc gcacggaggc gggggccctc ctggagaccg acctcgtgtt gctctcgatt
    10801 ggcgtgaggc caaccgtcga attagcggag cgctccgggc tggcgctcgg gcgcagcggc
    10861 ggaatccagg tcgatcgcgc gctgcgcacg agcgatccgg atgtgtacgc agtgggcgac
    10921 gcgacggagg tcacccaggg tgtcagccat ttcgcaaccc gcgtgccctt ggccggcgcg
    10981 gcaaaccgcc agggtagaat cgccggggag cacgcggcca gcggtcaggc gccgccctca
    11041 cctcaggtac tcggtaccgc gatcgtgcgg gtgttcgggc tcgaggtcgg agtgaccggg
    11101 cttggacggg ccgccgccgc tcaagccggt tactccgtag gcaccgcgct ggtgcatccc
    11161 aaacaccacg ccggctatta ccccggcgcg caacaaatgc atttcgagct ggtctatgac
    11221 gaggcaaccg gcaaagtgct tggcgctcag gcggcgggcg gcgcaggcgt agacaagcgc
    11281 ctggacgtga tcgcaactct attgcatttc ggtggcaccc tcgaggatct cgcggcgctc
    11341 gacttggcgt atgcgccgca attttcgagc gccaaggacc cagtgcacat ggctgcgttc
    11401 gtcgcgcaga acgatcgcca gcgcctctgc ccgagcacgg atgaagtgcc ccgcggagtg
    11461 cagttgctcg acgtgcgcac gcgccccgag ttcgagcgcg gtgctttacc caacgccgtg
    11521 aacattccac tcgatgagct ccgcgagcgt ctcggtgaac tcgaccctca cgagcccgtg
    11581 gtcgtctact gtgaggtggg cattcgaggt tacgccgcca cgcgcattct tctgcagcgt
    11641 ggcttcaggc atgtccaaaa cctcaaaggg ggatacctgc agcagtcccg caccgccagc
    11701 tgacgctacg cggttagtgt tcctacgagt tcgaaagtag caaaccggcg cccgaaccta
    11761 ttagcagcgc ttcgtctgca ccgggtgccg gttgctcgcc ctcgccgagc acacgcagaa
    11821 tgaatcgagc ctcgtcttgc gtggggatca agctgacccg cagggcgggg cactgagcca
    11881 tcgcaaaccg atcgtccagg cttgtaccca cattgggctg agtcgctccg acttcgggcg
    11941 cgacgtttgg aggtgccaca tcttgattcg aatggttggc tgagctcggc ggctctgcaa
    12001 aactcggcgg ccctgcaaaa ctcggcggcc ctgcaaaact tggcggctcc gctgaactcg
    12061 actgcggcag tgggctggtg ctaccgggct ggttcgatgc gcgcccaacg ctcggcggct
    12121 tggggggagc cttggcggaa atcgaaacgg cccgttgcag cgcctcggcg gccgcgttgg
    12181 actcgactgg tggcataggc agcggcggtg gcggctcgcg cggcggcggc tcgggcgtga
    12241 cctgcactgc agctagcgga agcggcggtg gaatcacctc cggatgagcc tcgacgaccg
    12301 cttccggcac ttggctctcc tcttcctcgt ccgcatccac gtacgcggtg gcatcttccc
    12361 actcgatatc gagttgggac aagcgcggcg catcgacctg cttatcctgc attcgcgttt
    12421 cggactctcg cgtgagaatc tccggaacga gcaccgggct catagctgcg ggggggaccg
    12481 cttgcgccac cggcgcactg agggccgcgg gctcgaccgc tacagggacc gggatcggcg
    12541 cgaccatcga ctcggggcta gctcggtgcg attgagagtc cgtctgatgc tcgactgttt
    12601 cgtcctccgt gcgctgcgcg ggagccggcg ctgcgccgaa gggagctttc tcctcgggca
    12661 agggcggagg ggtcgggacg ctgcgtcgcc ctggagcgct gctttccgcg tcgacgtttt
    12721 ccgtcgagac cgtcacggcc gctcggcttc gctgcacttc ccctccgcgc cccggcagca
    12781 gcggatcacc gaacagttca tcgaagatcg cgtcgacctg ggcttttcgc ggaggcgaat
    12841 ccgcctgcgc caacagcttc gtcacatcac gggccgactc ggacaagctc gcggctcgag
    12901 cctgcaattg cgactgcaga gcgagttccg ccgcgcgctc cagccagttt gcggtggcgg
    12961 cgagatccga tgctgcatgg ctcgcctcgg cactgagcag cgccctgcgc acgggctcgg
    13021 gatccgtggg atccggcttt ggtaccgcaa actccgctgt gggggtgcgc ttcatcatcg
    13081 actgcccttc ccgaaccggg cggagcgtag aacaaccagc gtttcgaaac cagcagcgcg
    13141 gaagcggaaa ttaaggtgac acctggtcag ctgcgaccgc cgtggctgtg agtgcgatgg
    13201 caccgttgga agcgatgacc ttgcggtaaa attcgtacga ttgtttaggg attcgccgct
    13261 gggttgcgta gtcg
//
LOCUS       DASTKU010000606         4226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1680249, whole genome shotgun sequence.
ACCESSION   DASTKU010000606 DASTKU010000000
VERSION     DASTKU010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680249"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1170)
                     /locus_tag="VFQ61_21190"
     CDS             complement(124..1170)
                     /locus_tag="VFQ61_21190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957031.1"
                     /translation="MFLKSFWVGALGSILLATAACNIKVEKADGDTEGGAPSGNGSGG
                     SSSTSGGARAAMGGADTAMGGAQVAMGGTSVGGASSSGGTGTASSGGAAPQVVEDCTG
                     KVTKTRVDTAHAADLGAGATICLNRDGDDYFFVDTPKDKGAHVLKLVIAPDAGADAQF
                     EAVAQSDASSMGRDFTTNGARTLWLALTGSTRTFLKFSPFSSGGRVVITPTWVPTQDS
                     YEPNDTKDTAGAIQVNTDTNAQLQRSYSTAADTTCVDWYKMELAAGPHSFVLSHVPDD
                     VRPQISVIGPSGENVKGDFGRNAGALFESAFTAATAGTYWLGITDFDGCPRSYYVGTT
                     LDSMTDVYTFKVTE"
     gene            1637..1834
                     /locus_tag="VFQ61_21195"
     CDS             1637..1834
                     /locus_tag="VFQ61_21195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957032.1"
                     /translation="MAVSVNESLGFAASHLDDWRKSHRSPGVRRLPDYRCQVLDKAIL
                     AIAHAVERFLHVVEIGDGDPG"
     gene            2031..2534
                     /locus_tag="VFQ61_21200"
     CDS             2031..2534
                     /locus_tag="VFQ61_21200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HET9957033.1"
                     /translation="MKNSAEPAPRASVTEDVNLFLVHAIQLERDAAHRFDELMHCMQT
                     GGNAELERLFRRLGELSRLHLKAAMERGGFREQPQLLPSEFQWPNGTTPEAMEWRGVD
                     AQLDVTSALELALDGEKTGCAWYSAVASGTDNPEVLAMAREFAEEELEHVSELERWIS
                     RQTRRRG"
     gene            2608..2829
                     /locus_tag="VFQ61_21205"
     CDS             2608..2829
                     /locus_tag="VFQ61_21205"
                     /inference="COORDINATES: protein motif:HMM:NF043052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HET9957034.1"
                     /translation="MSDHVYRIIELVGTSPVSQDEAIRNAIQRAGETMSPLRWFEVRE
                     SRGQIVEGKVAHWQVTVRIGATLPNTSDK"
     gene            2982..>4226
                     /locus_tag="VFQ61_21210"
     CDS             2982..>4226
                     /locus_tag="VFQ61_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9957035.1"
                     /translation="MLDLSSHYTADGTRQTTAAEWLARGLYEISQILITTPAPDSLGR
                     VLETLCSLLELRGALLTFVDSDGLRTTEESSWPLQPTGELRGWAHQVIEQVLNDSMPV
                     VVENAWVELAPQPGSDVEHVESELDVSLVGVPIVSGDKLDGALVILRQHSAGDQAAFS
                     FDSDVRLMSSVARISAVSWQLANVSAAEPVPEVAPHPPGDSAAIGRAMCWRAALHTAQ
                     TAAKTNATVLLRGETGVGKSLVARLVHEASPRHERPFVAINCAALPENLLESELFGHE
                     RGAFTGAMNRRLGRFELAHTGTLFLDEIGEISTAFQAKLLRVLQQGEFERVGGTETIS
                     VDVRVVAATHRDLEAAVRAGTFRADLYYRLCVVPVRVPALRERPQDIALLARTFLDRF
                     NAENGTDRSLDDTALAALGAHPF"
BASE COUNT          750 a         1335 c         1328 g          813 t
ORIGIN      
        1 gctgtacgcg cagaacagtg agcccgcgga acaggagcta ggtgcttctt tccgcgggct
       61 tcgctcttgt ttagccaaga ggagcgggtc gtgatcgcgt gcgactcgac cccgccggtg
      121 cgttcattcg gtgactttga acgtgtagac gtcggtcatc gaatccagcg tcgttccgac
      181 gtagtagctc cgagggcagc cgtcgaaatc ggtaatgccg agccaatagg tcccggcggt
      241 cgcggcggtg aacgcgctct cgaacagagc gcccgcgttc cttccgaagt cgcctttcac
      301 gttttcgccg ctgggcccga tgaccgagat ttgcggccgt acgtcgtccg gcacgtggct
      361 cagcacgaaa ctgtgcggcc ccgccgcgag ctccattttg taccagtcca cgcacgtggt
      421 gtcggcagcg gtcgaatacg accgttgtag ctgggcgttg gtatccgtat tcacttgaat
      481 cgcccccgcc gtgtctttcg tgtcgttcgg ctcatacgag tcctgagttg gtacccaggt
      541 cggcgtgatc accacgcgcc cgccgctcga gaacggcgag aacttcagga aggtgcgcgt
      601 gctgccggtt agcgccaacc acagcgttcg ggctccgttt gtcgtgaaat cccggcccat
      661 cgacgaagcg tcgctctgtg cgacggcctc gaattgggca tccgcgcccg cgtcgggggc
      721 gatcaccaac ttcaggacgt gagcgccctt gtccttgggc gtatcgacga agaagtagtc
      781 gtcgccgtct cgattcaggc aaatggttgc acccgcgccg aggtcagccg cgtgagcggt
      841 gtcgacgcgc gttttagtca ccttgccagt gcaatcctcg acgacctgcg gcgcagcgcc
      901 gcccgagctc gctgtccccg tgccgccgct cgatgatgcg ccacccaccg aggtgccgcc
      961 cattgcgacc tgtgcgccac ccattgcagt gtcggcgccg cccattgcag cgcgtgcccc
     1021 gcccgaagtc gaggaacttc ccccgctgcc gtttccggac ggcgcacctc cctcagtgtc
     1081 accgtccgcc ttttcgacct tgatgttgca tgctgccgtc gccagaagga tgctgcccaa
     1141 cgccccaacc caaaaagact tcaagaacat cccatactcc tcgaaaagac aacccgatgc
     1201 ggcgagcgct gtcgcccgct cctcgcaaaa ccactcaacc agtgccgggt gagtagcacg
     1261 atttcgaacc ttgggttgta cgcttcgagc gcgcgttcct tccttacgcg acgagaattc
     1321 cgtccgcgtg cccggcccat gagtccgaat cggcacgaaa ttgcgcggaa acgcggcgca
     1381 gataacgtta ccagaaagcc cgcttcttcg attcccctgc catctgcgtg cgaacaggtt
     1441 gaggtcgact cacgccctcg tcagcgtgaa acagctttag ttgcgtcgcg acgacatcgc
     1501 tccgcttgac gggctcgtcc tgaatgccgg cgaactcgtc gtttcggtaa tccagaccat
     1561 acttggagat ctgtggcgat cgtcttcgtc acgcttcttt ccggggccgt cgccccttac
     1621 gcaccaagct cgcgcgatgg ccgtctcagt gaacgagagc ctgggtttcg cggcctcgca
     1681 ccttgatgat tggcgcaagt cgcatcgaag cccgggcgtg cgtcgtcttc ccgactaccg
     1741 ttgtcaggtt ctggacaagg cgattctagc catcgctcac gcggtcgaac gctttctcca
     1801 cgtcgtggag attggcgacg gggaccctgg ttgatatccc cagcagttac ggctgaatgg
     1861 gtttcagctc ggcgacgaac gagctccgaa ttttgcagcg ggcgcacgcc gcatctccag
     1921 gtcgccagcc gagagttcct tggcatggtt cctgctcggg cgcgatttca gatcgagaaa
     1981 gaggagggcg ccgcgcgcga ccgtgcccag agcgctacgc catgataacc gtgaagaact
     2041 ccgctgaacc tgcgcctcgg gcctccgtga cggaggatgt gaacctgttc ctggtgcatg
     2101 ccattcaact ggagcgcgac gccgcgcatc gcttcgatga gctcatgcat tgcatgcaaa
     2161 ccggtggcaa cgccgagctg gagcggctgt ttcgtcgact cggtgagctc tcgcggctgc
     2221 atctcaaagc agccatggag cgaggcggtt ttcgagagca accacagctt ttgccctcgg
     2281 agttccaatg gcctaacggg acaactccgg aagcgatgga gtggcgaggc gtagacgcgc
     2341 agctcgacgt cacgagcgca ctagagttgg cgcttgacgg tgagaagacg ggctgcgctt
     2401 ggtactccgc ggtagccagt ggcaccgaca accccgaagt actggcgatg gcgcgcgagt
     2461 ttgcagaaga agagttggag cacgtttccg agctggaacg ttggatttcg cgtcagacgc
     2521 gccgccgagg ctagccctgg cccgcgaact ggaccgcgga gtccacaccg aaacagactt
     2581 ggtctccgtc acgaaaggaa cgcctttatg tccgatcacg tctaccgcat catcgagctc
     2641 gtcggcacct ctcccgtcag tcaggacgag gctatccgca acgcgatcca acgcgcggga
     2701 gagaccatgt ctccgctgcg ctggttcgag gtgcgggagt cgcgaggtca aatcgtggaa
     2761 gggaaggtcg cgcactggca ggtgaccgtg cgaatcggcg ccactttgcc gaacacgtcc
     2821 gacaagtgac gcgcccggtg tttctcccgt gtaacgcaga tttcggggga tgtggaccta
     2881 cgagtcacgc gtccgaaagg ctcccgtaat gggcacgagc aaagcatcca acctacagcc
     2941 tggtgagagc gtcccgggct gtacaaaaag gagcggcgtc ggtgctcgat ttgtccagcc
     3001 attacacagc ggacggaact cggcagacca cggcggcgga gtggcttgct cgcgggctct
     3061 acgagatctc tcaaattctg atcacgacac cagcgccgga ctcgttgggc cgagtgttgg
     3121 aaaccctgtg ctcgctgctc gagctgcggg gcgccctgtt gaccttcgtg gattcggacg
     3181 gccttcgcac cactgaggag agctcttggc ctctgcagcc cacgggggag ttgagaggat
     3241 gggcgcatca ggtcatcgag caggtcttga acgattcgat gccggtggtc gtcgagaacg
     3301 cctgggtcga gctcgcgcca cagccaggct cggacgtcga gcacgtggag tccgagctgg
     3361 acgtctcgct cgtgggagtg cccatcgtca gcggtgacaa gctggacggg gcgctggtga
     3421 tcctacgcca gcacagcgcg ggggatcagg ctgctttttc gttcgactcc gacgtgcggc
     3481 tcatgagtag cgtcgcgcga atttcagcag tgagctggca gctggcgaac gtttccgctg
     3541 cggagcccgt gccggaagtt gccccgcatc cgcccggcga ttctgcggct ataggccgcg
     3601 cgatgtgctg gcgcgcggcg ctccacactg cgcagacagc cgccaagacg aacgcgacgg
     3661 tgctgctccg cggggaaact ggcgtcggaa aaagcttggt ggctcgcttg gttcacgaag
     3721 cctcgccgcg gcacgagcga ccgttcgtcg caatcaactg tgcggccttg ccggagaacc
     3781 tgctcgagtc cgagcttttc ggccacgagc gcggcgcctt cacgggcgcc atgaatcggc
     3841 ggcttgggcg cttcgagctc gcgcacaccg ggacgctgtt cttggacgag atcggtgaga
     3901 tctcgaccgc gttccaggca aagctcctgc gcgtgctaca gcagggcgag ttcgaacgcg
     3961 tcggaggcac tgaaacgatt tcggtagacg tgcgcgtcgt cgccgcaacg caccgcgatc
     4021 tcgaagctgc cgtgcgcgcg ggcaccttca gggccgacct ctactaccgg ctctgcgtcg
     4081 taccggtgag ggtaccggct ctgcgcgagc gtccccaaga cattgctcta ttggcccgca
     4141 cctttctcga tcgcttcaac gcggagaatg gaacagacag atccttggat gacacggcgc
     4201 tggctgccct gggcgcgcat cctttt
//
LOCUS       DASTKU010000607         4322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_786731, whole genome shotgun sequence.
ACCESSION   DASTKU010000607 DASTKU010000000
VERSION     DASTKU010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786731"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFQ61_21215"
     CDS             <1..231
                     /locus_tag="VFQ61_21215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957036.1"
                     /translation="PGCLFGKGKFDERGQMALVFGGASIEQAQEELQFLSPFCGHGLS
                     RERVREVLVQPGCAMIVVIAHVALIKMNMSVR"
     gene            782..1762
                     /locus_tag="VFQ61_21220"
     CDS             782..1762
                     /locus_tag="VFQ61_21220"
                     /inference="COORDINATES: protein motif:HMM:NF016629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HET9957037.1"
                     /translation="MSELKQPHDALFKKMFSSVLYASSELASVLPVTLVRQLDFSTLA
                     LCAGAYVDEALAGSQSDLLFSAQINGRASFVYLLFEHQSQVDALMPLRLMKYVLRVLE
                     QHVERAGGSSRALPLPLVVPVVLYHGEKRWTAARSLQDLFDLEQVAASGVGELLPQLG
                     FVLDDLTEQSDSALESRRLHLATLVTLWLLRDARNRARFRRAREHWIPLLALVLDLPN
                     GAQLLETFFRYIGVVANEEEAQALSEGLQAYRPEAKEVLMTLAEKWFSEGEAKGKAEG
                     KAETLRKLLTLKFGELPAAVSERLSSATEGELDDAIERVLSATTLDQVVL"
     gene            complement(2790..3209)
                     /locus_tag="VFQ61_21225"
     CDS             complement(2790..3209)
                     /locus_tag="VFQ61_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957038.1"
                     /translation="MAALSSWIWCTHGSTPSNVRASYRGIALKTTLAAPIAYVFACAT
                     ILAIGSIICRVLYGLPIRAFASSASETVNGADARFGVLSMLAQILILVAAARFAAPKL
                     AGVSWGLLRKLIVGWVSLAVLRTVVAHISDGVWNGLR"
     gene            complement(3494..3916)
                     /locus_tag="VFQ61_21230"
     CDS             complement(3494..3916)
                     /locus_tag="VFQ61_21230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957039.1"
                     /translation="MATESEEALLAKCHSDWGSYPERVASCFVGTLGECLAAGCEGDD
                     DGCYTDALVAYDPSIVDIDSYEACRSSDDAAGCDDLTRGYLKDCLARTDECNVWDDLC
                     ASAVALKQPYRGEAEACLSRSCEELEACLYAAMGRRAP"
BASE COUNT          873 a         1302 c         1324 g          823 t
ORIGIN      
        1 ccgggatgcc tgttcggtaa gggcaagttc gacgaacgag gacagatggc gctggtattc
       61 ggcggcgctt ccatcgagca agctcaggaa gagcttcagt tcctctcgcc cttctgtgga
      121 cacgggttga gccgcgagcg cgtccgggag gtgctcgtcc agccaggttg tgcgatgatc
      181 gtcgttattg ctcatgttgc tctgataaag atgaacatga gcgtcaggtg acccaaccgt
      241 tcggcgtgca gccaattggc aagcttcgaa ggcagaactc ctcgcgcgag atctcgacgg
      301 acccgctcga tgagcggctc gagctcaatc tgaagcggct gatcgggaca acccttggac
      361 atagtgagga ctaccttggg tcggttcggc gcgctcggca accggaggcg cgatttccgg
      421 gcggttgtgg gttaccgctc gatgaaccag tcgattgggc atccagcaca gcgacaagtc
      481 ctgtgtcttg gccctactcg gactggtgct gagcgaatgc ggcgcaagat ggggtggcgc
      541 cagcgaaatc tccagcgaac tcgcgcgcgg tgctccgcct cgggagctga attgactcca
      601 agttcgacgg ccttgggcct ccgctgattc agaattggtt gtgcgcagtg ttttgagtag
      661 tcgtggtgtc tcgcgcatgc gcggccctcg ggatgtcccg gagtcctcga gtcgaatatc
      721 tattattttg ttcatcggaa ctcgttgcat actcggagat tggatctatt agatagcggc
      781 catgagcgag ctcaagcaac cgcacgacgc gcttttcaag aaaatgtttt cgagcgtgct
      841 ttacgcctca tcggagctgg cttcggtgtt gccggtgacc ttggtgaggc agctggattt
      901 ctcgacgctg gcgttgtgcg caggggccta tgtcgacgag gcgctggcgg ggtcacaatc
      961 ggatctgctg ttttccgccc agatcaacgg aagggcgtcg tttgtatact tgctcttcga
     1021 gcatcagagt caggtggatg cattgatgcc attgcgcctg atgaagtacg tgttgcgcgt
     1081 cctagagcag catgtagaac gcgctggcgg ctcgagccgt gctttgcctt tgccactggt
     1141 cgtcccggtg gtgctctacc atggcgagaa aaggtggacg gcggcgcgga gcctgcaaga
     1201 cttgttcgac ttggaacagg tagccgccag cggagtgggc gagctactcc cgcaacttgg
     1261 attcgtgctg gacgacttga ccgagcagag tgactctgcg ctagagtcgc ggcggctcca
     1321 cctggcaact ctggtcacgc tgtggttgct gcgggacgct cggaaccgcg cccgattccg
     1381 acgggcaagg gagcactgga tccccctgct ggccctcgta ctcgacctgc caaacggcgc
     1441 ccagctactc gagacatttt tccgctatat tggcgttgtc gcaaacgaag aagaggccca
     1501 agctttgagc gaaggcttgc aagcgtacag gcccgaagcc aaggaagtcc taatgaccct
     1561 cgcagaaaaa tggttctcag aaggagaagc caaaggcaaa gctgaaggca aagctgaaac
     1621 actgcgaaag ctattgaccc tgaagttcgg cgaactgccg gcggccgttt ccgagcggct
     1681 gtcttcggca accgaaggcg aactcgatga cgcgatcgag cgagtcctaa gcgctacgac
     1741 cctcgaccaa gtcgtgcttt gaggtttggc agccgctctg ccacgacgtt cgcaaacgcc
     1801 aagctcacgg cctaaaacgc gctcagttgc actgacggaa gccacggcca cagccttcgt
     1861 cccaaatcag cgggtatcaa ggatatcaaa gatatatcag gatatatcaa ggatgctgtg
     1921 cagggatggg tcttcatgct catgcaatgg caatgatcgc cttcggaatg gtcggctgcg
     1981 gcgcgtcgcg cgagactcag gcgagacgcc cagcgcccca ccattctgcg gccgctatgt
     2041 cacggacgct tgcggcgccg cgtgtatctt caagcccggc gctcgccatc cttgcgcact
     2101 tccccccgtt cgggccctta cggttgtgga agtgcgagct gccgaaacag gatatgtcct
     2161 caactcacgg cggtccttct gccgcggcgg cctgaacact cccatccaga cgacagacga
     2221 acgggccgag ctggagcgca tcatctcagt gctggccgtg gcccaccggt ccagagcgga
     2281 cccgtgcaaa accgctggac gtgacgcgac ggtttgggtg gtcgcacgcg ccccatcgga
     2341 tccggaaagg caagcccgaa tgttccggcg cgtcgaggtc ggaggcgagt gcggcgagtt
     2401 tcagatagca gcgcgcagtc taatgagact cgccggcctg gagtgtgaga gtagagcctg
     2461 tgttcgggcg cctgaacgtg ccgaccacaa gttcttatgt ccttgaacgg aaacctcgtg
     2521 agcttcacct tcaagggacg ttctctttcc ggatatcgcg ggcggcgccg gtcgggcacg
     2581 ctgtgagggc tctcgccgca gatagcgtct accgatcgaa acgaaaagcg ccctacgatc
     2641 gagggcgcgt tgagcgcgta tggctggttg acccgctcct tctcagattc ggagggttac
     2701 tcggacgtcc ccgagcgctg ccgcgagctt cggtctcttc gtggcgacta gctcctattg
     2761 ggctaggttc ttagccatga gacggagcgt cacctcagtc cgttccaaac gccatcactg
     2821 atgtgagcga caacggtgcg caggaccgcc agcgaaaccc accccacgat cagctttcga
     2881 agcaagcccc acgagacgcc ggcaagcttc ggcgcggcga accgcgcggc cgcgaccaag
     2941 atcaaaatct gagccagcat tgagaggacg ccgaatcgcg cgtccgcgcc gttgacggtt
     3001 tctgatgcgg aggaagcgaa cgcacggatc ggcaggccgt agagcacgcg gcaaatgatg
     3061 ctgccgattg cgagaatggt agcgcacgcg aagacgtacg cgattggtgc ggccaaggta
     3121 gtcttcaacg caatgcctcg ataggaggct cggacgttgg agggagtcga cccgtgcgtg
     3181 caccaaatcc agctggagag cgcagccacg accacggtgc ccgccgagaa ctctcgaacg
     3241 gtgctcagtg ccaggaccct aggaatgtat tcatgcccgc caaacttcga gaggcctaat
     3301 cccagccgaa cggtctcgga ggtcccggcg agtgccccga gaatgccgag tcatgcagta
     3361 ttagctcgaa agaacccggc gtgctgagta ggtgcgtccc gagtcgaatc caataggggt
     3421 tgcgtccttt cgagaaactc gatcagcgaa atcgtttgcc aaccaatcgc cggagttccg
     3481 gcgctgagcg cgactatggc gcccggcggc ccatggcggc gtagaggcac gcctcgagct
     3541 cctcgcagct acgcgacaga cacgcctccg cctctccgcg gtacggctgc ttcaatgcaa
     3601 ctgcgctcgc gcacaggtca tcccacacgt tgcattcgtc cgtgcgggcg agacagtctt
     3661 tgaggtagcc gcgcgttaga tcgtcgcagc ccgcggcgtc gtcactcgat cgacaggctt
     3721 cgtagctatc gatatccacg atgctcggat cgtacgccac gagcgcgtct gtgtaacagc
     3781 cgtcgtcgtc gccctcgcag ccggcagcga ggcactctcc gagggtgccg acgaagcacg
     3841 aggcgacgcg ctcaggatac gacccccaat ccgaatgaca ctttgcgagc agcgcctcct
     3901 ccgattcagt cgccattccg cacatacgct ctcgccgaac ccaaactcga cagagatcgg
     3961 tttccagccc gaccaccgag caggaacccg cctgctcggg tgccttttcc gtctcgtcac
     4021 cgcacgagaa gcatgccaga gcaagcacaa gcaaggatcc caaaactcca catttcccaa
     4081 taagcatcgc gcagtagagt cgctccactc tgctcgactg tcaacgcaat ggcggcagcc
     4141 gctgaattcg cggtgctgtt agttccccaa cgacggggga ccaacttgcg gtgggcccca
     4201 acgaagcgac atctttccca ctcagcgtag gatcatccaa ctgacgaaac gcagactcgc
     4261 cgggcacgtc gactcgaagt caggatggcc cgagagcctt gacggcgcgc ccaccacttg
     4321 gc
//
LOCUS       DASTKU010000608         5419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_786789, whole genome shotgun sequence.
ACCESSION   DASTKU010000608 DASTKU010000000
VERSION     DASTKU010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786789"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VFQ61_21235"
     CDS             <1..647
                     /locus_tag="VFQ61_21235"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HET9957040.1"
                     /translation="SLAEHREFLDGFLDQVLPREPLLLVLHDWGSALGFEWARRHPER
                     VLGVAYMEAIVGVREWDAFPPPARELFQALRSPRGEQLILEQNVFVEQLLPGSILRKL
                     TPEEHDEYRRPFREPGEARRPTLAWPRQLPIAGEPAHICREVERYAEFMARTNIPKLF
                     VNAEPGRSLTGPLRDACRRWPNQTEVTVRGLHFIQEDSPNEIATALAAFAKEVC"
     gene            641..1495
                     /locus_tag="VFQ61_21240"
     CDS             641..1495
                     /locus_tag="VFQ61_21240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9957041.1"
                     /translation="MLNASPNDLHALADAAKAGDRRALEALVRALQGSLYRVALRFLG
                     HPAHAQDATQEILILIVTRLGTFRGESTLQTWAYRIATRHLFRQRRTARQVTFESLVE
                     EDLAKPPNQVDPLALASADQRLLEEEVFVGCTQAMLQALDPPLRMAFVLGAILELEAS
                     DAAAALEISEPAFRKRLSRAREALDNFVSRHCGVANPANACRCAYQINHNLQKGRLNP
                     TQLRFALPAARKNPDALRTFGDIQRVRTALELYRSQPEFQAKEDFAQRLRELIESAPT
                     LSSSGSLS"
     gene            1761..2528
                     /locus_tag="VFQ61_21245"
     CDS             1761..2528
                     /locus_tag="VFQ61_21245"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HET9957042.1"
                     /translation="MEPQKISVLEWHRLLMGTTPAAFLLEMLVRCLVVYTFFVLIVRV
                     LGKRMSGQVANLELGVMITLGAIVSVPLQVAERGIVPALVLLLAVLALHQLLSWGTAR
                     YFKMEQLVQGHAKLLTKNGELMLDELREVGMSQEQLFSVLRSEKLRHLGEVKRVYLEA
                     CGTFSIIRNPTPHPGLSVLPRWDTPLQNRIPRAEELCACRFCGHVVLGSESETACARC
                     SRTEFTAAVAGAPETDEARRNSQQRDSGEQSSPEAAE"
     gene            2525..3214
                     /locus_tag="VFQ61_21250"
     CDS             2525..3214
                     /locus_tag="VFQ61_21250"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HET9957043.1"
                     /translation="MKPEDFRLLDLTRLVWGNTPWTFAIEVALRILVLYLILVIAMRA
                     MGRRMASQLSRNELIAMVSLAAGIGPAIQAPDRGLIPPAFIAVWVVLVQRAISAATVR
                     SYRIENLLQGEPATLVSDGTMDLRELQRNRISRNELFAQVRSIGALQLGAIERIYFEP
                     NGTFTVIQSAQPTPGLTVVPSWDEDLKAEQRLSEDRLVCHHCGKLADRQDTSRACPVC
                     KAKSWQPAVEA"
     gene            3526..>5419
                     /gene="treZ"
                     /locus_tag="VFQ61_21255"
     CDS             3526..>5419
                     /gene="treZ"
                     /locus_tag="VFQ61_21255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HET9957044.1"
                     /translation="MSWTRRFPIGAELAMGDGVHCRVWAPNRKKVMVVERQPGKPDPG
                     RKQALTREPGGYFAGLVPFLRAGSLYSFQLDDEDLLLPDVASRFQPEGPHGPSQIVDP
                     RSYAWKDTDYRGPWSTARVVYEMHIGTFTAEGTYRAAQRELPELRALGVTTLELMPVA
                     DFPGRFGWGYDGVDLWAPYHSYGPPDALREFIDSAHRLGLEVILDVVYNHLGPDGNYL
                     KQFSPHYFSERYENEWGEPLNFDAEHSGPVREFFTSNARYWVEEFHFDGLRLDATQSL
                     FDASDTHIIVDVTRAVNFAARAQNKAAYVVAENEPQHTRLVRPYNTGGFGCSALWNDD
                     FHHTARVALTGRHEAYYHDYRGSPQELISALKWGYLYQGQNYSWQKKCRGTPALDLNA
                     RNFVSYLQNHDQVANSATGARLHELTSPAQLRAMTALMLLAPPTPMLFQGQEFASSAP
                     FLYFADQKPELCEGLNRDRRKFLSQFPSIAHPDVADRLADASALETFERCKLNFAERV
                     QHQTVYDLHRDLLQLRHTDPVFNQQRADVMHGAVLGSASFVLRWMMESGERLVLVNLG
                     MDQAIDPAPEPLLACPEGSTWELVWSSEHPKYGGIGFGKVFDAGRWQLPAHSTHVFKA
                     VPEDATD"
BASE COUNT          998 a         1735 c         1656 g         1030 t
ORIGIN      
        1 attccctggc cgagcaccgg gagtttttgg acggcttctt ggaccaggtc ttgccgcgag
       61 aaccactgct cctcgtattg catgactggg gctctgccct tggcttcgag tgggcccggc
      121 gccacccgga gcgcgtgctc ggcgtcgcat acatggaagc catagtcggg gtgcgcgaat
      181 gggacgcctt ccctcccccc gctcgcgagc tctttcaggc tttgcgatcc ccccgcggtg
      241 aacagctgat cttggagcag aatgtgttcg tcgagcagct tctgcccggc agcattttgc
      301 gaaagctcac cccggaagag cacgacgagt accggcggcc cttccgcgaa cccggtgaag
      361 ctcgccgccc cacgttagct tggccccggc agctgcccat cgccggcgaa ccggcgcaca
      421 tttgccgcga ggtggaacgc tatgcagagt tcatggcgcg tacgaacatc cccaagttgt
      481 tcgtcaacgc ggagccaggg cgaagcctga ccggtccctt gcgagatgct tgccggcgtt
      541 ggcccaatca aactgaagtg acggtgcgcg gtttacactt cattcaagaa gactccccca
      601 atgagatcgc gaccgcgctg gctgcgttcg caaaggaagt atgttgaacg ccagccccaa
      661 cgacttgcat gccctcgccg atgcggccaa ggcgggagat cgccgcgcgc tcgaagcatt
      721 ggtgcgtgcc ctacaggggt ccctgtaccg cgtcgcgctg cgattcctgg gtcatcctgc
      781 ccacgcccag gacgcaaccc aggagatctt gattctgatc gtcacccggc tgggtacgtt
      841 tcggggtgag agcacgctgc agacttgggc ttatcgaatc gcgacgcgac acttgttccg
      901 acaacgacgc acggcgcgcc aggtcacctt cgagtccctg gtggaagagg acctcgccaa
      961 accgcccaac caggtggacc ctctcgcgct cgcgagtgcg gatcagcgac tcttggagga
     1021 ggaagtgttc gtgggctgca cccaggccat gctgcaagca ctcgatccgc ccctgcgtat
     1081 ggcgttcgtg ctcggtgcga tcttggagct cgaagcgagc gatgcggccg ccgcgctgga
     1141 gatctcggag cccgcctttc gcaaacgcct atcgcgcgcg cgggaggcgc tggacaactt
     1201 cgtgtctcgc cactgcggcg tcgcgaatcc cgccaacgcc tgccgctgtg cgtaccagat
     1261 caatcacaac ctgcagaaag gacgactgaa ccccacacag ctacgtttcg cgttgcctgc
     1321 ggcccggaag aaccccgacg cgttgcgcac gttcggcgac atccagcgcg tgcgcacggc
     1381 gctcgagctg taccgttctc aacccgagtt tcaagccaag gaagactttg cccagcggct
     1441 gagggagctg atcgagtccg cgccgacgct ctccagttcg ggctcgctat cttgagagga
     1501 cgacggggcg tttgactcga taccgtaata ggcgcagccc acccgagtgt caggccgcca
     1561 cgcgggtgag tttacccgcg tggcggcttc acgccggctc gccccgcgaa ggccgcatag
     1621 ccacggccta ggctccttgg ctcacatttc gcaacacgcg cgtcccaacg gtccacgcct
     1681 ccccaaaccc gcgcgacacg ccacatgcga ttgtcgccag aagttggcga cgcgtgaggt
     1741 ggatgcgtcc cagaggagcc atggaaccgc agaaaatttc cgtactggag tggcatcgct
     1801 tgctcatggg gacaacgccc gcggcgtttc tcctcgaaat gctcgtgagg tgcttggtcg
     1861 tctacacgtt cttcgtgttg atcgtccgcg tgctcggcaa gcgaatgagc ggtcaggtcg
     1921 caaacttgga gctgggagtg atgatcacgc tcggcgccat cgtctccgtg cctttacaag
     1981 ttgccgaacg tggcatcgtg cccgccctgg tactgttgct cgcggtcctg gcgttgcatc
     2041 aactcctgag ttggggcacc gcacgctatt tcaaaatgga gcagttggtt cagggccacg
     2101 ccaagctgct gacgaagaac ggagagctga tgctggacga gctgcgcgaa gtcggcatgt
     2161 ctcaagagca actgttctcg gttctgcgca gcgagaagct tcgccacctc ggcgaagtga
     2221 agcgagttta tctggaagct tgcggcacat tctcgatcat tcgcaatccc acgccgcacc
     2281 ccggcctctc ggtgttgcca cgttgggaca cgccgctaca aaaccgtatc ccccgggctg
     2341 aagagctttg tgcctgcaga ttctgcggac acgtagtgct cggcagcgaa tcagagaccg
     2401 cttgtgcacg ctgctcgcgc acggagttca cggcggccgt ggcgggagcg ccggaaacag
     2461 acgaggcccg ccgaaacagc cagcagcggg atagcggaga gcaaagctcg ccggaggctg
     2521 ccgaatgaag ccagaagatt tccgactcct cgatctgacg cggctggtct ggggaaacac
     2581 cccgtggacg ttcgcgatcg aggtcgcgct ccgcatcctc gttctgtatc tgattctcgt
     2641 gatcgctatg cgcgccatgg ggcggcggat ggcgtcgcaa ttgagccgca atgagctgat
     2701 cgcaatggtg tccttggccg ctgggatcgg gccggccatt caagcgcccg atcgaggttt
     2761 gattccgcca gcattcatcg cggtatgggt ggtgctcgtg caacgcgcga tctcggccgc
     2821 gaccgtgcgc agctaccgga tcgagaatct gctccagggt gagccggcca ctctggtgag
     2881 tgacggaacc atggatctgc gtgagctcca gcggaatcgg atctctcgca acgagctctt
     2941 tgcacaagtg cgatccatcg gcgcgctgca gctcggcgcg attgagcgaa tctatttcga
     3001 accgaacggc acgttcacgg tgatccagtc agcccagccg acccccggcc tgaccgtggt
     3061 gccctcgtgg gacgaggatc tgaaagcaga acagcgcctg agcgaagatc ggctggtttg
     3121 tcatcactgt gggaagctcg cggaccgaca agatacgagc cgagcttgcc ccgtttgcaa
     3181 ggcgaagagt tggcagccgg cggtggaagc ttgagcgctg ccgaatccgc ctcctcgatc
     3241 ctccccgttg acgacctcgg ctgaatgcca accgtgcttt ccctcccgta accaccactt
     3301 tcgcgatccg gccttgcgcg agttcacgct gcgcgtccga gcacggtttg tgctcatcgt
     3361 gttggtcgct ttgtccccgt cgcacatatt cgtgaggttc agaaggcagc cgggtcttcc
     3421 gtcgagtctc gtcgcgggga tcctcagcca atctgcacgt gacgttcttg cgaagcgggg
     3481 cgcgcttgtg atttcaacct cgaaccaagg ggcaattggc tgaacatgtc ctggacccgc
     3541 aggtttccga taggtgccga gttggcgatg ggtgacggcg ttcactgcag agtctgggcg
     3601 ccgaatcgca agaaggtgat ggtggtcgag cgacagccgg gcaagcccga tccagggcgg
     3661 aaacaagcgc tcacacgcga gccgggtgga tacttcgcgg ggctcgtgcc gttcttgcgc
     3721 gcaggcagcc tgtactcctt ccagctcgac gacgaggatc tactgctgcc cgacgtcgcg
     3781 tcgcgtttcc agccggaagg tccgcacggc ccctctcaaa tcgtcgatcc gcgaagttac
     3841 gcctggaaag acacggacta ccgcgggcct tggagcaccg ctcgcgtcgt gtatgaaatg
     3901 cacatcggca ccttcaccgc cgaaggcact taccgagcag cccagcgcga gttgcccgag
     3961 ctccgcgcgc tcggcgtcac cacgctcgag ctcatgccgg tcgcggattt tcctgggcgc
     4021 ttcggctggg gctatgacgg ggtcgacttg tgggcgccct accactctta tggcccgccc
     4081 gacgcgcttc gcgagttcat cgacagcgcg catcgcttgg gtctggaggt gatcctggat
     4141 gtcgtctaca accacctcgg cccggacggt aactacctga agcagttctc gccgcattac
     4201 ttcagtgaac gttacgagaa cgaatggggt gaaccgctaa acttcgacgc ggagcactcc
     4261 ggccccgtcc gcgaattctt caccagtaac gctcgctact gggtagaaga gtttcatttc
     4321 gatgggctcc gcttggacgc aacgcaaagc ctattcgacg cttcggatac tcacatcatc
     4381 gtcgacgtga cgcgcgccgt gaacttcgcc gcgcgcgccc agaacaaagc agcctacgta
     4441 gtggccgaaa acgagccgca gcacacgcgt ctggtccggc catacaacac cggcggcttc
     4501 ggctgctcgg ccctttggaa cgatgacttc caccacaccg cgcgcgtggc cctcaccggt
     4561 cgacacgaag cctactatca cgattatcgc ggcagccctc aggagctgat ctcggcccta
     4621 aagtggggat acctgtatca aggtcaaaac tacagctggc aaaagaagtg tcgcggcacg
     4681 ccggctttgg atttgaacgc caggaacttc gtctcgtacc tccagaacca cgatcaggtc
     4741 gcgaacagcg cgaccggcgc ccggctccac gagctcacga gcccggctca gcttcgcgcg
     4801 atgaccgcgc tgatgttgct cgcgccgccc acgcccatgc tgtttcaggg ccaggagttc
     4861 gcatcgtcgg cgccattcct ctacttcgcg gatcagaagc ccgaactctg cgaggggctc
     4921 aaccgtgacc gtcgcaagtt tttgagtcag ttcccatcga tcgcccatcc cgacgtcgcc
     4981 gacagattgg ccgacgcctc tgcgctcgaa acgttcgagc gctgcaagtt gaacttcgcg
     5041 gagcgcgtcc agcaccagac agtttacgat ctgcaccgcg atttgctgca gttgcgacat
     5101 acggaccccg tgttcaacca gcaacgcgct gacgtcatgc acggcgccgt gctcgggagc
     5161 gccagcttcg tactgcgctg gatgatggaa tcgggcgagc ggctcgtgct cgtcaacttg
     5221 ggcatggacc aagcaatcga tcccgcaccg gaaccgctcc tcgcctgccc ggaggggtcc
     5281 acttgggagc tggtttggtc gagtgaacat cccaagtacg gcggaatcgg ctttggaaag
     5341 gtgttcgacg cgggtcgttg gcaattgccc gcgcactcca cgcacgtttt caaggcggtg
     5401 ccggaggacg caaccgatg
//
LOCUS       DASTKU010000609         5963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_564294, whole genome shotgun sequence.
ACCESSION   DASTKU010000609 DASTKU010000000
VERSION     DASTKU010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564294"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VFQ61_21260"
     CDS             <1..874
                     /locus_tag="VFQ61_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957045.1"
                     /translation="ALATNVTDRYQTALDLQADIEVYCERLGGAVRQKEIGNLVSSLF
                     AETRAELKALIERRLAQVTTDDTFGDLSASEVRSTISGSRQITPISMSNAQSAAQPGP
                     AENKKSKYAIVAGLAVLLLGVGYYLESTRAREAAAPAPQPTVNAAPEAPKAAPAAPAA
                     PTLATLQLRATPTDAKLFLDDELLPSNPTSKVLPIDGKVHRLRAEADGYAASVAEFSA
                     TRDDTVEVALSEIPSSPKRGAARYIPRAASRPAPEPAKPAATPAPVPEKRPACDHPFF
                     VDSDGIKKIRPECM"
     gene            complement(976..2547)
                     /locus_tag="VFQ61_21265"
     CDS             complement(976..2547)
                     /locus_tag="VFQ61_21265"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET9957046.1"
                     /translation="MRRVGIFGWGVVAPKSPNIEEFAKNLAGSESWLSAFDGFGPDRF
                     LVGNPSFDFNDYKPWIDARFAPSRYRQLIEKADTNSLYAIGAFIQALGQNPGLEGALS
                     SLGEQAHVYVGTGLGSLNTAYDVSVNLYRAQRRWNRFWSEPSRNPALASYLTERRLPA
                     GADVPEEPEAIQDPERREMAEDAWFAFWAARSSALSEYLDDLGEIESMTVEGEVKAGK
                     LALLREKSRRLARLNEKWSAPEPPWTRVSPNLLWNISNAPAAQITMLGHISGLSFAPV
                     AACSTFGVALKLAMDSIDRGQAKLVVVGATDGAPNPLSVAAFYNARVHAADGQVSKPL
                     SGLRGTHVSGGACVWIVGDLEYAQEHGFRPLGLEPVAVGTTSDAEHIITPSEEGPRAA
                     IRQALTQAGCGPEELVSWDLHATATPGDHQEVENLRHVLGNEVIVTARKGTFGHGMAA
                     AGGWELTAQYLGCASGALFPTPLSESELNPEIRKLHERFAFREPLVIPRGPVGKLSMG
                     VGGINACVISRPWDT"
     gene            complement(2865..3074)
                     /locus_tag="VFQ61_21270"
     CDS             complement(2865..3074)
                     /locus_tag="VFQ61_21270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957047.1"
                     /translation="MSLPPFVLRAPTLTDCSGAGTVAGVRSPNCSRLDRGPCALLDLA
                     SARAVPMQTRQREGVLVVTARARST"
     gene            3744..4826
                     /locus_tag="VFQ61_21275"
     CDS             3744..4826
                     /locus_tag="VFQ61_21275"
                     /inference="COORDINATES: protein motif:HMM:NF014116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HET9957048.1"
                     /translation="MMNRRTWTTIGVVAFALLGSIAGCSDDDDVSISTGGKGGTGGAK
                     GGGGSGGTGGTGTAAKGGTTNKGGTTSTPEGGMTSEGGDTGTTGGTKASGGTTSKGGT
                     TGEGGATMEGGAGGAGGDGGAGGAEGGATMMLGGMTSMGGTTAAGGTTAAGGTTAAGG
                     TTAAGGTTAAGGTTAAGGMTADGGTTTAGGTTAAGGTTAGGGTAGTGGTTGASNLIID
                     GTFEDGSKGTWGGWSQTGLAATMTVAHSGSYSLTAAVQATGVIAKDIKSLVTAGARYQ
                     ATAWVYFTGPESAQIMLQTVQNCDAAASDSYPWLQGDTKTQGTWTQLTGVVNLTTCTS
                     VDKLLLFVGSAAAVNLYIDDISLTPL"
     gene            complement(4968..>5963)
                     /locus_tag="VFQ61_21280"
     CDS             complement(4968..>5963)
                     /locus_tag="VFQ61_21280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957049.1"
                     /translation="ALPRTEPMPQVAPTASNSVAVTAAERAVVALRPEVPEVAHEVFF
                     PAFNKLENVTYTPGLYARAKLHFVDKKLGLDAWQSYERWVPVLPTDADPRFSAGSLVP
                     PIFSTNIAPAPLPGSQFEPLPPCLQVAKKYAAFRKAFAQYLYESEAFVLWRAPALKLS
                     SEPGETEPQFRARVEVAAREARDEALEDLQEDYREEFEKLQAKLEKAQAKLDRERAQS
                     REQALDTALSVGTGLLGALLGRKVASQANVRRAGRVGRSGAKAARARVDVAQAEQSRE
                     LLEQAIEELEREFHSESARVSAELDPAHIDIEEQRIPPRKADINVETLQLAWRPD"
BASE COUNT         1142 a         1890 c         1935 g          996 t
ORIGIN      
        1 ggcgctcgcc acgaacgtga cggatcgcta tcagacggcg ctggatttgc aggcggatat
       61 cgaggtctac tgcgagcggc tcggtggggc cgtgcgacaa aaggagatcg gcaacctggt
      121 gtcctcgttg tttgccgaga cgcgcgccga gctcaaagct ctgatcgagc ggcgtctggc
      181 gcaggtaacg acggacgata ccttcgggga tctctcggcc tccgaggttc gcagcacgat
      241 ctcgggctcg cgccagatta cgcccatctc gatgagcaac gcgcagagcg cggcgcagcc
      301 gggcccggcg gagaacaaga agtccaagta cgcgatcgtc gcgggtttgg cggttctctt
      361 gctcggcgtc ggctactatc tagaatccac acgcgcgcgc gaagccgcgg caccagctcc
      421 gcaacccacc gtgaacgcgg cgccagaggc gcccaaggcg gcaccggccg cgccagcggc
      481 accaacactg gcgacgctcc agcttcgagc cacgccgaca gacgcaaagc tatttttgga
      541 cgatgaactt ctaccgtcga atccgacgag caaggtgctg ccgatagacg gcaaggtgca
      601 tcgtctgcgg gcggaggcgg acggctatgc cgctagcgtc gcggagttct cggcaacgcg
      661 agacgatacc gtggaggtcg cgctctcgga gattccgagt agcccgaagc gcggcgcggc
      721 ccggtacatc cctcgcgcgg cgagtcgacc cgctccggaa cccgcgaagc cggcagcaac
      781 gccagcgcca gtaccggaga agaggcctgc ctgcgaccac ccattcttcg tggactcgga
      841 cgggattaag aagattcgtc ccgagtgcat gtagtcggac ccgcccgtcc gctgggcctc
      901 ggcgagtggc atgcggcgga tgcagccccg aacgggcggt gcaatgttgc tgccgtcagc
      961 gccaaagtgc ggcgctcagg tgtcccacgg tcgtgagatc acacaggcgt tgatgccgcc
     1021 gacgcccatg ctcagcttgc cgaccgggcc gcgcggaatg acgagcggct cgcgaaacgc
     1081 gaagcgctcg tggagcttcc ggatctccgg gttcaactcg ctctcggaca agggagttgg
     1141 aaatagcgcg ccgctcgcgc agcccagata ttgagccgta agctcccagc cccccgcggc
     1201 ggccatgccg tggccgaacg tgcctttgcg agcagtcacg atcacctcgt tgccgagtac
     1261 gtgccgcaag ttctcgacct cttgatgatc gcctggagtg gccgtcgcat gcagatccca
     1321 agacacgagt tcttcgggcc cacatccagc ttgcgtcaat gcctgccgaa tcgccgcgcg
     1381 agggccctct tccgagggag tgatgatgtg ctcggcatcg gaggtcgttc cgaccgcgac
     1441 cggttcgagc cccagcgggc gaaagccgtg ctcctgcgca tactctaggt caccaacgat
     1501 ccacacgcag gcgccaccgg atacgtgggt gccgcgcaaa ccggagagtg gcttactcac
     1561 ctgaccgtcg gccgcgtgaa ctcgtgcgtt gtagaaggcc gctacggata gcggattggg
     1621 cgccccatcc gtcgcgccga ccacgaccag cttggcctgg cctcgatcga tgctgtccat
     1681 cgcaagcttc aaggcgaccc cgaaggtcga gcacgccgcg accggcgcga acgagagccc
     1741 gctgatgtgg cccagcatcg tgatctgcgc ggcgggggcg ttgctgatgt tccagagcaa
     1801 attgggtgac acgcgcgtcc acggcggctc gggcgcactc cacttttcgt tgaggcgggc
     1861 gagacgtcgg ctcttttcgc gaagtagcgc gagcttgccg gccttgacct ccccctcgac
     1921 cgtcatcgac tcgatctcgc ccaagtcgtc caggtactcc gaaagcgcgc tcgagcgcgc
     1981 tgcccaaaac gcgaaccacg cgtcctcggc catttcccgt cgctcgggat cctgaatggc
     2041 ctctggttcc tctggaacgt cagcgcccgc aggcagcctt cgctcggtca agtagctcgc
     2101 tagtgcggga tttctcgacg gctcagacca aaatcggttc cagcgccgct gcgcccggta
     2161 caagttgacc gacacatcat aagcggtgtt gaggctaccc aggcccgtcc cgacgtagac
     2221 atgcgcttgc tcgccaagcg aggacagcgc cccctcgagc ccaggatttt gacccagcgc
     2281 ctgaatgaag gccccgatcg cgtatagcga gttcgtgtcc gccttctcga tcagctgccg
     2341 gtaacggctc ggcgcgaagc gcgcgtcgat ccacggtttg tagtcgttga aatcgaaact
     2401 aggattgccg accaagaagc ggtcaggacc aaagccgtcg aaggcactga gccagctctc
     2461 cgagcctgcc agattcttcg cgaattcttc gatgttgggg ctcttggggg cgacgacgcc
     2521 ccaaccaaaa atgccaacgc gacgcaatcc tgattctcct tcggagcagt aaccacgccg
     2581 cctcggagtt cgtcaatgcc cgaaccagca caagtccagg gtcgcccggc aacgcaatgc
     2641 gtcggggcgc atgaatcatg caatttttgg ttgatgttcc gtccggtttg acgacggcct
     2701 cgcttcgcaa gcgagtggac ctggtccttc gcgactgcaa acgattcggc actgcgactt
     2761 cgttcgactc ggtttccggc aagtggaggc ttactttccg cagcggcgtt ttagggcctt
     2821 agaactttga cgctcgaagc ggcgctccaa acccgaggag tggatcacgt cgatcgagct
     2881 cgcgccgtga ccaccagcac tccttcgcgc tggcgagtct gcatgggcac cgcccgcgcc
     2941 gacgcgaggt cgagtagcgc gcacggccca cgatccagtc gtgagcagtt gggcgagcgc
     3001 acccctgcga ccgttccggc gcccgagcaa tcggtgagcg tcggtgcgcg taacacgaac
     3061 ggcggtaggg acatggcgcg aagagcggga gtgcgaagac ccgtgtgccc ggacgagtga
     3121 cagtcgatcg ccgcaatcga cgctcataag acaaactgca ctcgagtcac gactcaaaca
     3181 cgaagacgaa tcaagggact tggacggagc tcgacgattc gatcgtgtga cgtgaatcat
     3241 cgtcgtgatc ggatgagtca aactgacgga aaccgcacgg acgcggtatt cgcgagacct
     3301 gagttgaaat cgcaggcctc ggcgaaaatc gccgtacgat gacgagattg cagagccgag
     3361 ggcggctgcg aaaacgcccc gaggatcgcg gaacgggaga gtgcgaaccg cgctcgaaga
     3421 agcgacgcag atgcggggga caacagagag caaacgtggg gacgaggtgc agatgcgtca
     3481 cgctttcacg acatgaaaac gggggcgttc ggctccaaag ttcgatcgaa aattcacgct
     3541 cgctgatcgc ctgtcgcaca cggtgatcca cgcgctcact tcttcgaagc gagccacacg
     3601 cgagcactgc actttagcga attagttcgg taacgtaccc gctctacctg gacgggttgc
     3661 caatgcgtct tgctctcgga agcgcccgat cgcgccgacg gttactcgga ctcggacagg
     3721 ccaatcagga agggaaactt gaaatgatga atcgaagaac ttggacgact attggtgttg
     3781 tggctttcgc attgctgggc tcaatcgcgg gatgttccga tgacgacgac gtgagcatca
     3841 gcaccggcgg caaaggcggc accggcggag cgaagggtgg cggcggcagc ggcggaactg
     3901 gcggaaccgg cacggcagcc aaaggcggca ccaccaacaa gggcggcacg acttccaccc
     3961 ctgagggtgg catgacgagc gaaggtggcg acaccggcac gaccggcgga accaaggcga
     4021 gcggcggcac tacgtccaag ggcggaacca cgggcgaagg cggcgccacg atggagggcg
     4081 gtgctggcgg cgcaggcgga gatggaggcg cgggcggtgc agagggcggc gccacgatga
     4141 tgctcggcgg catgacctct atgggcggaa ccaccgcagc aggtggaacc accgcagcgg
     4201 gcggaaccac cgcagcaggc ggaaccacgg ccgcaggcgg aaccaccgca gcgggtggaa
     4261 ccacggcggc aggcgggatg accgcggacg gcggtaccac gaccgcgggc ggaaccacgg
     4321 ccgcgggcgg aaccacggcg gggggtggca ctgcggggac cggcggcacc accggggcga
     4381 gtaacctgat catcgacggc accttcgagg acggcagcaa gggcacctgg ggcggttgga
     4441 gccaaactgg cctcgcagcc acgatgaccg tcgcgcacag cggttcgtac agcctgaccg
     4501 ccgccgttca agcgaccggt gttattgcca aagacatcaa gtctctggtt acagcgggcg
     4561 cgcgctacca ggccacggcg tgggtctatt tcacaggccc cgaatcagcg cagattatgc
     4621 tgcaaacggt tcagaattgt gacgcagccg cctcggatag ctatccatgg ctacagggcg
     4681 acactaagac tcaagggacg tggacgcaac tcactggggt tgtcaaccta acgacctgca
     4741 cgtcagtcga caaactgttg ctgttcgtcg gctcggccgc ggccgtgaac ctgtacatcg
     4801 acgacatcag cctaaccccc ctgtgagctc caggctctag ccctggcttg gcagagagcc
     4861 gggctgatcc gacatagtcc gtcgtagtcc tgattgaacc gagtccgcac agaggggcca
     4921 gctcccacgc tcatcatcgc gtaggttggc ccctcgagcg cctcggctca gtctggtcgc
     4981 caggcgagct gcagggtctc gacgttgatg tccgctttcc tcgggggaat gcgttgttct
     5041 tcgatgtcga tatgcgccgg gtccaattcg gcgctgactc gcgcagactc ggagtggaac
     5101 tcgcgttcca gctcctcgat cgcttgctcg aggagctctc ggctttgttc ggcttgcgcg
     5161 acgtccacgc gcgccctcgc ggctttagcg ccgcttcgtc ccactcgacc cgctcgccgc
     5221 acgttagctt gagacgcaac cttgcggccc agcaacgcgc ccaatagccc cgtgccgacg
     5281 ctgagcgcag tgtctagtgc ctgctcgcgc gattgagctc gctcgcggtc gagcttcgcc
     5341 tgcgcctttt cgagcttggc ttgcagcttc tcgaattcct cgcggtaatc ctcttgcaga
     5401 tcttcgagcg cttcgtcacg ggcttcgcga gcggcgactt ctacgcgcgc gcggaactgc
     5461 ggctcggttt cgcctggttc cgagctaagc ttcaaggccg gcgcacgcca caggacgaaa
     5521 gcttcgctct cgtacaggta ttgcgcgaaa gccttgcgaa aggcggcgta cttcttcgct
     5581 acctgcaagc acggcggcaa aggctcgaac tggcttcccg gcagcggagc cggtgcgatg
     5641 ttcgtcgaga agataggcgg aacgaggctg cccgcgctaa agcgaggatc cgcgtcggtt
     5701 gggagcactg gcacccagcg ctcgtagctc tgccaagcat ccaagccgag cttcttgtcc
     5761 acgaagtgca gcttggcgcg ggcatatagg ccgggcgtat acgtgacgtt ctcgagcttg
     5821 ttgaacgcgg gaaagaatac ctcgtgcgcg acctcgggca cctccggtcg gagcgcaacc
     5881 accgctcgct cggcggccgt gactgccacg gagttggacg ccgtgggtgc aacctgcggc
     5941 atgggctcgg tgcgcggcag ggc
//
LOCUS       DASTKU010000610        16145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1905039, whole genome shotgun sequence.
ACCESSION   DASTKU010000610 DASTKU010000000
VERSION     DASTKU010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16145
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905039"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="VFQ61_21285"
     CDS             <1..1144
                     /locus_tag="VFQ61_21285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9957050.1"
                     /translation="LVRRSSMFWKKNSVEHKVAGGAEESSGLDQAEVALDTIASMLRS
                     YGQNAFDTEEVRAAETQSECESWAQKITVGPSRKDGAAGEFKRDFGGVRRYFTAHRAR
                     ERDYVDRSFSNLRDAVQTFAHCLTKAISEERSSDQNVSGQLQRLTQAFTSNDATAIRK
                     QAETVVELVRSAIQERQRRQAEQVEVLGQKIQKLRDELQQAREQASLDPLTQLHNRSA
                     LDSHLERVADLAFLLNSSPCLLMIDVDHFKSVNDKHGHPAGDEVLRKIADALVRQFLR
                     REDFVARFGGEEFVVVIPDSTLQNAKKRAERVREAIQELEIPHQGKVLKVTISVGISC
                     LAEGDAGKSWLARADAALYESKAAGRNRVSVASFPVHSLASLRPSS"
     gene            complement(1147..2211)
                     /locus_tag="VFQ61_21290"
     CDS             complement(1147..2211)
                     /locus_tag="VFQ61_21290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9957051.1"
                     /translation="MRVLFVTRKYPPMVGGMEAQSYQLTSRFPAPKRIISLGRSQKHL
                     VWFLPYVLVRAWLEADTYDVLHLGDGLLAAVGLAPRYLAGKRVVVTVHGLDLTFRSRL
                     YQTYLKLCLRPHQALPVSESTRRIAKERGLCAPYSIPVGVTEPFFDIPRDPGADAELN
                     AQRAGRTILLTIGRLVPRKGVAWFIRNVLPKLSGVLYVVVGDGPDASAVREAIRSSGT
                     EQSVLLLGRISAERLRSVISAADIFVMPNISVPGDVEGFGIVAIEAAASGLPVVASRL
                     EGIPDAIIDGKNGDLVNPGEPDAFVAALTPLLQQPRLRAQRGDAARQFTRETYAWPRV
                     VERYVQALSEQLSPDARPRE"
     gene            complement(2236..2511)
                     /locus_tag="VFQ61_21295"
     CDS             complement(2236..2511)
                     /locus_tag="VFQ61_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HET9957052.1"
                     /translation="MARQVNCIQLGHEAEGLDKPPFKGDLGQRVFDNVSKEAWRRWLE
                     HSKMLINEYRLDLTSPNGQRIWMTECEKFFFGDGSSAPPEFVPKDAG"
     gene            complement(2567..2788)
                     /locus_tag="VFQ61_21300"
     CDS             complement(2567..2788)
                     /locus_tag="VFQ61_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957053.1"
                     /translation="MTRISALGLGLFALLTALPAAARPADCGDEKKEKNPSALSTNSC
                     GEEKKKDPAALPQSGSQCGDEKKKDPAAS"
     gene            complement(2820..3482)
                     /locus_tag="VFQ61_21305"
     CDS             complement(2820..3482)
                     /locus_tag="VFQ61_21305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957054.1"
                     /translation="MACVKLRAVLGRLLVACSLVTLPFFGMRSVLASTADRLGTSLGE
                     WAALEANWLKPVLVAAPPVAPASPPPIYEPEPTPIAPEEAPALQAPSGSAKKSKKTPR
                     KPATSGAPAPARGVFVSAANVLRLAQARAVPHAVFVPASGARPAGLQLAGVGGLGIGM
                     RDGDILTRVSGAPVSSVGEVVARVIAARAQRAARIGGEFWRNGEVYNLIVEQPYVAET
                     AP"
     gene            4410..6098
                     /locus_tag="VFQ61_21310"
     CDS             4410..6098
                     /locus_tag="VFQ61_21310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957055.1"
                     /translation="MTSNANTANSSDQRSALLPGAPGAPGTRGEGAPWHVHLELQPEE
                     LEALERTGLTDGGLAWRKGMQGWQPLAAVPRPAELPESSSRWIPQRSLPARGESGRSE
                     TAREDSQSASQSQRGPVPPRKPPFKVGRNTPPLLSPVVVPLHPSLAREAGARELGARD
                     AAGNVAAGNEAAHNYAPPLQELLAPPIPGQPIGNPPPSGQRLSGMGLPVGNLPSFEGM
                     PPSLGPGFTGPAFSTSGVSAPSGLTGVGPVAESVPQSGLLRLKGKSKTTWLGAAALLL
                     LSASNGALVSALIWSMRRSETEKSAAFAEQIVAAAREAAVIAARDAVAQSAALSATRA
                     ASAANPDDTATNSKNEGGGVTSPEEVPSATSESRVNTRRAARVSVEQLPVVHSSRKAA
                     AVEAKESKPAAAEAKESKGAGEALPVARTGSARSALDAAIAKSQGINLTPPEATPSSD
                     KPSRHARGKANAKVELTEEPATRRGTAPGPADRAAIGSAVSRAAWAASSCGSGAHKGR
                     VSLTFSPSGSVQSVQLVQSFQDGNINACVLRAMGRAHVAPFVGAPVTVTKTISW"
     gene            6228..7163
                     /locus_tag="VFQ61_21315"
     CDS             6228..7163
                     /locus_tag="VFQ61_21315"
                     /inference="COORDINATES: protein motif:HMM:NF003738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HET9957056.1"
                     /translation="MSHASPSASSTESKLDPSIGKETAPNELDAAAALRELSDFFARG
                     PIFVLTGAGVSTDSGIPAYRDERGEWQRPQRPVEYRRFMDFEATRRRYWARSMLGWPS
                     FDRAQPNSAHRALAQLERAGWLSLLVTQNVDGLHQRAGSQRVVDLHGRLDHVICMQCR
                     VRTSREALQHRLIEDNPLFSNASSRVAPDGDADLADADYDQFRLAHCQACSGVLKPDV
                     VFFGENVPQERVERAMQALERSAGVLIVGSSLMVYSGYRFAKRALKLDIPVAILNRGR
                     TRADEFATLRVPHGAGEILTALVAQGLVPRKAERD"
     gene            complement(7179..7457)
                     /locus_tag="VFQ61_21320"
     CDS             complement(7179..7457)
                     /locus_tag="VFQ61_21320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957057.1"
                     /translation="MTTRTNKERCWPGYEPVPGKKPNEQGSCRKKPSSKTTASEKQFR
                     ARRQQQLDAWSAKHPNSPRKAAQHLHAPVEPRSKRAVSPKRRTKKLAA"
     gene            7618..8523
                     /locus_tag="VFQ61_21325"
     CDS             7618..8523
                     /locus_tag="VFQ61_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957058.1"
                     /translation="MWGLRLKHSFGIVQSGLIVLSLVFSACGSKTAPLDGYAQEPSPW
                     PEAPSDFVPGTREKPNGCTDEAQGIVLLSADGGLYRLSPPKLALTTIGKMSCDSPLTS
                     MSLARDGTFWGAGEDGLLYKSSGTGCESTGLNLRVKNEDRFGTGIVADENGRDHLFVA
                     PIGLRPVGKDTFVSNVPELIDVTLGQRPHLRHVPYSLDTSNMEFAGTGDSRLFALSDP
                     NLIVITQLDPSDGSTLEKHYISDLEYLGAYDFCYWQGDFYLFNSTSAGTSDVFRYRVS
                     TRQLEPVGTVPIVVIGAESTTCAPL"
     gene            complement(8557..8751)
                     /locus_tag="VFQ61_21330"
     CDS             complement(8557..8751)
                     /locus_tag="VFQ61_21330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957059.1"
                     /translation="MDETDFEGTLVLERLASIDMLEEFFDAVDSDDVQRAIVLMRRAK
                     IDASTIKIVVEKMERADGEH"
     gene            8750..9349
                     /locus_tag="VFQ61_21335"
     CDS             8750..9349
                     /locus_tag="VFQ61_21335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957060.1"
                     /translation="MVELPDSQLGSSRACFQPAIEKTWRTDSASCYRKPVKIGFSVHE
                     KVIGKRLGALARASVFAFACPLLSCNSSNDHAVSPASSSASESGAPAPSTDVPPFNGT
                     ESNWWLVQETAPTGRSDVLPAFEESARAYGCNTEPLGLDSVQDIYGERRTYHGISASC
                     REGTIALITLRGGRIRIGCVKPTTREACDRLLRNIADAR"
     gene            9414..9896
                     /locus_tag="VFQ61_21340"
     CDS             9414..9896
                     /locus_tag="VFQ61_21340"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET9957061.1"
                     /translation="MMSPEWLGALARYNRWMNDKLYALAATLSDDARKRDCGAFFKSI
                     HGTFNHLLVADRVWLSRFKGVPAPEGFMAPGIRSLDQDLYSEFEALRRERALTDDELS
                     AWSPGSPPSSSRPPSCSCAVVRGSRRPCGEPSRMFSITRPTIVDKSLLCSPNRARIQG
                     "
     gene            complement(10080..11318)
                     /locus_tag="VFQ61_21345"
     CDS             complement(10080..11318)
                     /locus_tag="VFQ61_21345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HET9957062.1"
                     /translation="MPEAARYSTETYCPTQSPGGAGGTMNDHETSVSKISRGTVSGDT
                     APDNRSLDAYRAIGDPVLDPLITSYSAARIGEMLGSLFQQDSLPLDDERFRPLLESLP
                     PVISNDPRALERGQRLFQLYGPEVLLILGCYGLPASYAAANGVQVLFRARRLADDTQR
                     RLCETAQMLINVMQPGALLDGGIGLRSTLKVRLMHALIRRHVELMQHLDPWLDDWGKP
                     INQEDLAGTLLTFSALVLDGLLRIGAPLSEAEQQGYLDTWCHLGRLLGIDERLLPVSL
                     PYARDLAARIARRQFRYSDEGRILVRDLIRVTNGLFPVPGYGLSLMHYFLDQSVFGMS
                     LAEILDLPKPNWTRYLVRARAAQKKLYFRLLAAVPGARKRRSRMAGKFVQALILLERP
                     DKQSPFEVPDELRSAWGLLS"
     gene            11516..12160
                     /locus_tag="VFQ61_21350"
     CDS             11516..12160
                     /locus_tag="VFQ61_21350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9957063.1"
                     /translation="MGDDEHASPPTPVPGTRQSVGIPEPPSRDLLLACKAQNQAAFRV
                     FVASYQRAVFALLSRTTGPGAHVQDLAQEVFLRAFRAFPRFDVDGSARASTWLLTIAV
                     RLALNARKGLSRAQQLYPLAEAEALPAAGTPESERTRRELGTAIAKAAQELSPEQRAI
                     FVLSEFHGMSISEIANAVEIPEATVKTRLFRARAQLRESLASFWSFPRERSHGA"
     gene            12150..12977
                     /locus_tag="VFQ61_21355"
     CDS             12150..12977
                     /locus_tag="VFQ61_21355"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9957064.1"
                     /translation="MERNMSKEWDSWEAQAPSHDFVDRVMHELRFEGTPVVPARSWRS
                     PRSLAMLVGAAALLASLALLAASLQQRWGTHSGSREKPQARSASLGDDGERRSDSAGP
                     INSGRGHNLDSGTRWNAHSERAATVDRELRARVSEKLAPRLRAHGVERDPHTGLTIPS
                     GQAGPVGNITREYIQQRIQKDFMPLALACYEAALAKHPALAGTLAIDFMIVGDPSVGG
                     IIDQAKINASSTLHDLELSNCMLGSLQSIVFAAPDNYGWVTVTYPFVLSPGSGSGEN"
     gene            complement(13161..14150)
                     /locus_tag="VFQ61_21360"
     CDS             complement(13161..14150)
                     /locus_tag="VFQ61_21360"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HET9957065.1"
                     /translation="MAPVGPAPASMKKSSSIRKWHLALIAPAVVLGAWVGWRAFSRGQ
                     PYSTPGGSRQVSALSASSRGAPSPPGALASDGGSIAPDPSAPSDVSAPPGDASVSAAG
                     VAYRVLKSGGGSSNPLPDDRVTVHYTSWKRTGTLVDSSRLRGEPYVQTLRYMVPGLAE
                     AIASMTEGEQRRIWVPARLTFASNDEKPHIPEDLTFEVELLRIRRAPVLPTGLRQAPR
                     TATRTASGLAYEFMKPRGPQGPAILPNTRVTFSHSVWTESGTLFESSEMSEQPVSSSV
                     STLIPGLREGLPLLRPGDRVRFWIPAALAYGDKPGRHQPGGALIYELELMKIE"
     gene            complement(14153..>16145)
                     /locus_tag="VFQ61_21365"
     CDS             complement(14153..>16145)
                     /locus_tag="VFQ61_21365"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HET9957066.1"
                     /translation="VNIATGADRAGSPKQITATVPGTGDGSDGTNVAFNPMFHNQRSG
                     LALASNAVYVAFAAYCDARPTHGWLMGFDVTSLASTGVFNTTPNGTLGGIWQAGSAPA
                     VDSAGNVYVTTGNGDFNGTKNFGETLLKLAPRTLTRTNSTSNFFTPSNFATLNAADDD
                     FGSAGPSFLPGTNLIVNGGKEGKVYLLNSSSLGGMVAGDAQIPQSFQAVDSAARPGDT
                     HHIHNHNVVWSSPAGINMYVWGENDYLRAYRFNSATQKFITPATLVGSVLPPPGMPGG
                     TMSLSANGSTAGTGILWATTPSQGNANQASVPAILRAFNAETLALLWDSSSPGDDVLG
                     LSKGSPPLIAKGKVYLASVSGAVSVFGARSGPTPPIANAIYQIRTRTTTGKCADIFNS
                     STADGGNLQQFTCNGTNAQRFQFINIANDIYEIRNVNSNKCLDVDNSGTADGTNIRQW
                     TCNGSNAQRWAVQALGNGQYRLMPQTAANRCLDVSNSGTADGTNIQQWTCNGSAAQTL
                     TLAFDNTGAQPIPNSTFRITTSTTSGQCLDIDNGGGGDGVNLHQWECNGSDAQRFRFR
                     SLGGNLYEVRASVSEKCLDVDNSGTADGTNIHQWECNGTNAQIWSVDSIGNGRYRISP
                     QVAANKCMDVDNSGTTSGTNIHEWTCNGTAAQQFSVIAP"
BASE COUNT         3020 a         5075 c         5076 g         2974 t
ORIGIN      
        1 gctagtacgg cgctcttcaa tgttctggaa gaagaattca gtagaacaca aggtagccgg
       61 aggcgcggag gagagctccg gactggacca ggcggaggta gcgctggata cgatcgccag
      121 catgctgcgc agctacggcc aaaacgcctt cgatacggag gaggtgcgcg ccgcggagac
      181 ccagagtgag tgtgagagct gggcgcagaa gatcacggtc ggcccgagcc gaaaagatgg
      241 cgcggccggc gagttcaaac gcgacttcgg cggggtgcgc cgttacttca cggcccatcg
      301 tgcccgcgag cgagactacg tcgatcgcag cttcagcaat ttgcgcgacg cggtgcaaac
      361 ctttgcgcat tgtctgacca aggccatctc cgaggagcgg agctcggacc agaacgtgag
      421 cgggcaactg caacgtctga cccaggcctt cacgagcaac gacgccaccg cgatccgcaa
      481 acaagccgag accgtggtcg agctggtacg ctcggcgatt caagagcgtc aacgccgcca
      541 agccgagcag gtggaggtgc tcgggcagaa gatccaaaag ttgcgcgacg agctgcagca
      601 agcgcgcgag caagcctctc tggatccgct gacccagcta cacaaccgct ccgcgctgga
      661 tagccacctc gagcgcgtcg cggatctggc gtttttgctc aactcgagcc cgtgcttgct
      721 gatgatcgac gtcgatcact tcaagagcgt gaacgacaag cacggtcacc ccgcgggtga
      781 cgaggtgcta cgcaaaatcg cggacgcgct cgtgcggcag ttcttacgcc gcgaggattt
      841 cgtggcccgt ttcggcgggg aagagttcgt ggtggtgatc cccgactcga cgctacagaa
      901 cgcgaagaag cgcgccgagc gcgtgcgcga agccatccag gagctcgaaa tcccgcacca
      961 gggcaaggtg ctcaaggtga cgatctcggt ggggatctcg tgcttggccg agggtgacgc
     1021 gggcaagagc tggctcgcgc gcgccgacgc cgcgctgtac gagtccaaag ccgcgggtcg
     1081 caatcgggtg agcgtggctt cgttcccggt gcactcgctc gcctctctgc gcccgagttc
     1141 ctgagctcat tcccgaggcc gcgcgtcggg cgacagctgc tccgagaggg cctgaacgta
     1201 gcgctcgacc acccgcggcc aggcataggt ctcgcgcgta aactggcgcg ccgcatcccc
     1261 gcgctgggcg cgcagacggg gttgctgcag caggggagtg agcgccgcca cgaacgcgtc
     1321 cggctccccc gggttcacca ggtcgccgtt cttaccgtcg atgatggcat ccggaatgcc
     1381 ctccaggcgc gaagcaacca cgggcaaccc gctcgcggcc gcctcgatcg cgacgattcc
     1441 gaagccctcc acgtctcccg gcacgctgat gttcggcatc acgaagatgt cggccgcgga
     1501 aatcacggag cgcagccgct cggcagagat tctaccgagc agcaaaacgg attgctcggt
     1561 gccgctgcta cggatcgctt cacgcacagc gctcgcgtcc gggccatcgc ccacgacgac
     1621 gtagagcacg cccgagagtt tgggcaacac gttccgaatg aaccaggcga cgcccttgcg
     1681 tggcacgaga cgcccgatcg tcagcagaat cgtccgaccc gcgcgctgcg cgttcagctc
     1741 cgcgtccgcg cccggatcgc gcggaatgtc gaagaacggc tcggtcaccc cgaccgggat
     1801 cgaatagggc gcgcacaacc ctcgctcttt ggcgatgcga cgcgtggatt cgctgaccgg
     1861 caacgcctga tgcgggcgca ggcagagctt caaataggtc tgatagagcc gcgatcgaaa
     1921 cgtgaggtcc aagccgtgga ccgtcacgac cacgcgcttc ccggccaggt agcgtggggc
     1981 gagccccacc gccgcgagca acccatcgcc taggtgcaac acgtcgtacg tgtccgcctc
     2041 gagccaggct cgcaccaaca catacggaag aaaccacact agatgcttct gcgagcgccc
     2101 gagcgaaatg atgcgcttgg gggcgggaaa gcggctggtg agctgataac tctgtgcctc
     2161 catcccgccc accatgggcg gatacttacg ggtgacgaac aggaccctca tcgcgctcgt
     2221 gcgctggagc gcgcttcaac ccgcgtcttt gggtacgaat tccgggggag ccgaggagcc
     2281 gtcaccgaaa aagaacttct cgcactcggt catccagatg cgttgcccat tcgggcttgt
     2341 cagatccagg cgatactcgt tgatgagcat cttggagtgc tcgagccagc gtcgccaagc
     2401 ctccttcgag acattgtcga acacacgctg ccccaagtcg cctttgaacg gcggcttgtc
     2461 gaggccctca gcctcgtgac ccaactggat gcaattcacc tgacgcgcca tgtgatttta
     2521 gggcggcacc atgacgctgc gccctaggct ggtcaactga gccgaatcaa ctggccgcgg
     2581 gatccttctt cttttcgtcc ccgcactgcg agccggactg cggaagggca gcgggatctt
     2641 tcttcttttc ctcgccacaa gagttggtcg agagggccga ggggttcttt tccttcttct
     2701 cgtcaccgca gtcggcaggg cgagccgccg ccgggagagc cgtgaggagt gcgaacagac
     2761 cgagaccgag agccgagatg cgcgtcatgg gagaatgatt ggcgcgtgag cccgcaacgt
     2821 caaggcgcgg tttctgccac ataaggctgc tccacgatca ggttgtagac ctctccgtta
     2881 cgccagaatt ccccgccgat ccgcgcggct cgctgcgcgc gcgccgcgat tactcgggcg
     2941 accacctctc ccaccgagct cactggggcg cccgaaaccc gcgtcaaaat gtcaccgtct
     3001 cgcattccaa tgccgaggcc gcccacaccg gcgagttgga gccctgcggg acgcgcaccg
     3061 gaagcgggaa cgaatacggc gtgcggcacc gcccgcgctt gcgcgagccg cagcacattg
     3121 gcggctgaaa cgaatactcc gcgcgctggc gccggcgcgc cgctcgtggc cggttttcga
     3181 ggcgtctttt tgctcttctt ggcgcttcca ctcggtgcct ggagtgcggg cgcctcttca
     3241 ggtgcgatgg gcgtgggctc gggctcgtag atgggaggcg gagacgcggg agcgaccggc
     3301 ggagctgcga cgagcacggg cttgagccaa tttgcctcga gcgcggccca ctcgcccagg
     3361 ctcgttccca ggcgatcggc ggtgctcgcg agcacgctac gcattccaaa gaaaggcaag
     3421 gttacgagcg aacaggcaac gagcagccgg ccgagcacgg cgcgcagttt aacacaagcc
     3481 aacgtcgcgc ccagatctag ccagttccgg gccccccaac caccgacgcg ccgcgcgtgg
     3541 agacgcgctt ttcccgtctc gacgggctgg tggtcgtacg cggatcgacg gcttggcgtg
     3601 gctgaaggct ggaatcggta ctggtcggga aagagcccca cccgaaatgg ccgtgagtcg
     3661 ccacggcccc ggcaattcat cgaggctggc agttggcatt ctctacgaaa ctccagatct
     3721 ccgaagacgc gcgatgtcga atcaagcaca ggccgcgttc ggtttgatgc gaacgaattg
     3781 ctgatgtgtc gtggcttgca tcggggtcgt ggattccagc agtccgggat gtttgcttca
     3841 ctggctcaac ctcagcgcct cggctgacgc acgctgcaca gacgaagagg gcagaagaga
     3901 agttgcatgc gcgcggagcg ggtgtttttc gagcgagttt ctcgacccaa cgctctcgct
     3961 cacgcaacgg gaccatggcg atcatgcgct cgctcccagc gccggcgaag ggtcgtaatc
     4021 cgcagccgat ttcgcggaat ttttcagagc atgcgtgctc ccagtccgcc cggtaagagc
     4081 gagtcgcaac ggcattgcag ctagtcaatc tgtgaaccac tgccaactcg atgtctctgg
     4141 ctggggcatg gtggtgattc cgtcgaagcc ggaaccgata ccactcgaga aaattcatcg
     4201 agctctgatg aggcgatctg cagagtgccc ttgcccccgc cttcgaatga gcctaaaggt
     4261 gggtgggcgt acggaaaggt ctcgaacgtg accctaggtg gacgacgtca agagctgatg
     4321 cgagctgtgt gttctgtagt gtgcggagtc gctcgccacg gctcactcgc gtgggtgatg
     4381 ttgccagagg ggaggctcga atgcgtgcca tgactagcaa tgccaatacc gccaatagct
     4441 ccgaccaacg gtctgcactg ctacccggag cgcccggagc gcccggaaca cgcggggaag
     4501 gagcgccctg gcacgttcac ctcgagctcc agcccgagga gctcgaggcg ctcgagcgca
     4561 cgggcctgac cgacggaggc ctggcctggc ggaagggcat gcagggctgg cagccgctcg
     4621 ccgccgtacc gcgccccgcc gagttgccgg agtcctcgtc gcggtggatc ccgcagcgca
     4681 gcttgccggc acgcggtgag agcgggcgat cggaaacggc acgtgaagat agccagtccg
     4741 catctcagtc gcagcgcggc cccgtaccgc ctcgtaagcc tccgttcaaa gtgggtcgca
     4801 atactccacc actcttgagc cccgtggtcg tcccgctgca tccgtctctg gcccgcgaag
     4861 caggcgcccg cgaactcggc gcgcgagacg ctgcgggcaa cgtggctgca ggcaacgagg
     4921 ctgcgcacaa ctacgctcct ccgttgcaag aactgttggc tccgcccatt cccggacagc
     4981 ccatcggcaa tccaccacct agcgggcaaa ggctctcggg tatgggttta ccggtcggca
     5041 atttgccctc cttcgagggc atgcccccga gtctgggtcc gggcttcacg ggcccagcgt
     5101 tctcgacgtc tggcgtctcg gcgccctcgg gcctcaccgg tgtggggcca gtagcggagt
     5161 ccgtaccgca atcggggctg ctgcgtttga agggcaaatc gaaaacgaca tggctcggcg
     5221 ccgccgcgtt gttgctcctc agcgcaagta acggcgcgct ggtttcggca ctcatttgga
     5281 gtatgcgccg ctccgaaacg gagaagagcg cggccttcgc ggagcagatc gtagccgcgg
     5341 cgcgtgaagc ggcagtaatc gcagcgcgag atgcggtcgc gcagagcgcc gcgctcagtg
     5401 ccactcgcgc cgcgagtgcg gccaacccgg atgacaccgc taccaactcg aagaatgagg
     5461 gcggcggggt cacctccccg gaagaagtgc cgtccgcgac cagtgaatcg cgagtgaaca
     5521 cgcgacgagc cgctcgagtc tcggtcgagc aactgccggt cgtgcactca tcacgcaagg
     5581 cggccgccgt ggaggccaag gagagcaagc cagctgccgc ggaagccaaa gagagcaagg
     5641 gcgcgggcga agcgctgccc gtggctcgca ccggtagcgc acgcagcgcg ttggatgcgg
     5701 cgatcgccaa gtcccaaggc atcaatctta cgccgcccga ggccacgccc agctctgaca
     5761 agccgagtcg ccatgcgcgc ggaaaggcca acgccaaagt cgagctgaca gaggaacccg
     5821 cgacgcgacg aggcacggcg ccgggccctg ccgatcgcgc cgcgattggc agcgccgtat
     5881 cgcgggcggc gtgggccgct agttcatgcg gctcgggcgc ccacaagggc cgcgtcagcc
     5941 tcactttctc gccctccggg agtgtgcagt cggtacagct ggtgcagtcg tttcaggacg
     6001 gtaacatcaa cgcttgcgtc ctgcgcgcga tgggccgcgc tcatgttgcg ccgttcgtag
     6061 gggcgccggt gaccgtgacc aagaccatta gttggtgaac agctacgctc cgagggaacg
     6121 cccactgcgc gtccgaaact gacgtccaat cacccggaac ggtcggttag acaagccgtt
     6181 ggcgagctgg caagcgggct gtgctcggct aaggttttca cgtggtcgtg tcgcacgcat
     6241 ctccgtctgc ttcgagcact gagtcgaaat tagatccgag tatcggcaaa gagacggcgc
     6301 cgaacgagct cgacgccgcc gccgccctgc gggagttgag cgacttcttc gcgcgaggcc
     6361 caatcttcgt gcttaccggg gcgggagtca gtacggactc gggcattcct gcttaccgcg
     6421 acgaacgcgg ggaatggcag cgcccccaac ggcccgttga ataccgtcgc ttcatggact
     6481 tcgaagccac gcgccgccga tattgggcgc gcagcatgtt gggttggccc agcttcgacc
     6541 gtgcccagcc caactcggcc catcgtgccc tggcccaact agagcgcgca ggatggctct
     6601 ctttactcgt cactcagaac gtggacgggc tgcaccagcg tgcgggtagc caaagagtcg
     6661 tcgatctaca cgggcgactc gaccacgtga tttgcatgca gtgtcgcgtg cgtaccagtc
     6721 gggaggccct gcaacatcgc ttgatcgaag acaaccccct gttctcgaac gcgagcagcc
     6781 gagttgcacc cgacggtgac gccgacctgg cagacgcaga ctacgaccaa ttccgactcg
     6841 cccactgcca agcatgcagc ggagttctca aacccgatgt cgtcttcttt ggcgagaatg
     6901 tgccgcaaga gcgcgtcgag cgagccatgc aagcgctgga gcggtctgcc ggagtcctga
     6961 tcgtcggctc atcactcatg gtctactcgg gctatagatt cgcgaagcgc gcgctcaaac
     7021 tcgatattcc ggttgccatt ctcaaccggg gccgcacccg cgccgacgag ttcgccacgc
     7081 tgcgcgtgcc acacggcgcg ggggagatat tgacagcgtt ggtggcgcaa ggtttggtgc
     7141 ctcgaaaagc ggagcgagac tgaccactcc gcatctcgtc atgcggcaag cttcttggtt
     7201 ctacgcttgg gcgacaccgc ccgtttgctt cgaggttcga ccggtgcgtg caggtgctgc
     7261 gccgctttgc gtggcgaatt ggggtgcttg gcactccatg catccagttg ttgttggcgc
     7321 cgcgcgcgaa actgcttctc actcgcggta gtcttggagc tcggcttctt gcggcaactg
     7381 ccctgctcat tcggcttctt ccccggcacg ggctcgtaac ccggccagca acgttccttg
     7441 tttgttcgtg tggtcattgg gtaggctcct gcacggggga tagattcctg cccgggtgtg
     7501 cttgcacggg acgtgccgtt cgcaccgcgc tgcggctttg gcggccagcc ctccccggca
     7561 ccttctggtt ccagtagcct ctggtgcggc gaagcggcag gaggcatagt tcggcgcatg
     7621 tggggtttgc gcctgaagca ctcgtttggg atcgttcagt ctgggttgat cgtattgagc
     7681 ttagttttct cggcgtgcgg ttcgaagacg gctcccctcg acgggtatgc gcaagagccc
     7741 tcgccctggc ccgaagcgcc gtccgacttc gttcctggca ccagggagaa gcccaatggc
     7801 tgcactgacg aagcgcaggg tatcgtgttg ttgtcggccg atggtgggct gtaccggttg
     7861 tccccgccca agctcgcgct caccacgatt ggcaagatga gctgcgactc tcccctgact
     7921 tcgatgtctc tcgcgcgcga cggcaccttc tggggcgctg gcgaagacgg tctcttgtac
     7981 aagagctccg gtaccggctg cgaatccacg ggcttgaatt tacgagtcaa gaacgaggac
     8041 cgattcggca cgggcatcgt tgccgacgag aacgggcgtg accacctctt cgtggcgcca
     8101 attggtttac gccccgtcgg caaggatact ttcgtttcca acgtcccgga gctgatcgac
     8161 gtaacccttg gccaacggcc ccacctgcgt cacgtaccgt attccttgga cacttccaac
     8221 atggaatttg cgggcaccgg agattctcgt ctgtttgcgc tgagtgatcc gaatctgatc
     8281 gtcattactc aactcgaccc gagtgacggt tcgactctgg agaagcacta tatctcggat
     8341 ctggagtatc tcggcgccta cgacttctgt tactggcagg gtgatttcta cctcttcaat
     8401 agtacgtcgg caggaaccag cgatgtattc cgatatcgtg tgtccacccg ccaactggag
     8461 ccggtgggaa ccgttccgat tgtggtgatc ggggcggagt cgaccacctg tgctccgctc
     8521 tgacgaccga aatctgacac ggaaacctga catcggtcag tgctcgccgt cagcccgttc
     8581 catcttctcg accacgattt tgatcgtcga tgcatcgatc ttggcgcgcc tcatcaacac
     8641 gatggcgcgt tgcacatcgt ccgaatccac cgcatcgaaa aactcctcca gcatatctat
     8701 ggatgcgagc cgctccagga cgagcgtgcc ttcaaaatca gtttcatcca tggtcgagct
     8761 cccagatagc cagcttgggt cctctcgcgc ctgctttcaa ccggcaatcg agaagacgtg
     8821 gcgaactgac tctgcctcgt gttatcgcaa gccggtgaag atcggtttca gcgtgcatga
     8881 gaaggtaatc ggtaaaagac taggtgctct agcccgcgct tcggtttttg ctttcgcctg
     8941 cccactgctg agctgtaaca gttcgaacga tcacgccgtc tccccagcat cttccagtgc
     9001 gtcggagagc ggcgcgcccg ctccctccac ggatgtgcca ccgttcaacg gaacagaatc
     9061 gaactggtgg ttggttcaag agacggcgcc gaccggacgt agcgatgttc ttcccgcgtt
     9121 cgaggaaagc gcgcgcgcct acggctgcaa caccgagcca ttggggctcg actcggtcca
     9181 agacatctac ggggaacgtc gcacttatca cggcatcagc gcatcttgcc gagaaggcac
     9241 catcgcactc attacactcc gcggggggag gatacggatc ggctgcgtaa aaccaacgac
     9301 ccgagaagcc tgcgatcgcc tccttcgcaa catcgccgac gcaagatagt gcgcagaggg
     9361 acgggcgctt ggtggcactt ggcagccagg atttcctgcc ctagtttagg tgcatgatga
     9421 gtcctgaatg gctgggtgcg ctggctcgct ataaccgatg gatgaacgac aagctctacg
     9481 cgcttgcggc cacgctgagt gacgacgccc gtaaacgcga ctgcggcgcg ttcttcaagt
     9541 cgatccacgg cactttcaac catctgctcg ttgcggaccg ggtttggctc tcccgattca
     9601 agggcgtacc tgcccccgaa gggttcatgg ctcctgggat tcgctccctc gaccaagacc
     9661 tctactccga gttcgaggcc ttgcgtcggg aacgagcact cactgacgac gagctctccg
     9721 cgtggtctcc gggctctcca ccgagcagct cgcggccccc ctcgtgttcg tgcgccgtgg
     9781 taagaggatc gagacgccct tgtggggagc cgtcgcgcat gttttcaatc accagaccca
     9841 ccatcgtgga caaatcacta ctgtgttcac ccaacagggc acggatccag gggtaacgga
     9901 cctgttcgcc atgctcctcg aggagcagca gcgggcacga taattagtta ccttgggcgc
     9961 caccaatccg cgccgcgccc cgccccaaac ggactgaggt ctggcaacgg ccaggtctca
    10021 gtccgactct gcgtaaagtt tgcagtgcgc ctcccccact ggacgggccg gtgggtgcct
    10081 caactcagga gcccccatgc ggaccgcagc tcatccggca cctcgaaagg agactgctta
    10141 tccggccgct cgagaagaat cagagcttga acaaactttc ccgccattcg gctgcggcgt
    10201 tttcgagctc cgggcacggc cgcgagcaat cggaaataca acttcttctg cgccgcccgc
    10261 gccctcacca aatatcgagt ccaattcggc ttgggcaagt ccaagatctc cgcgaggctc
    10321 ataccgaaga cgctctgatc caggaagtag tgcatcaaac tcaagccata ccctggcacc
    10381 ggaaacaggc cattcgtgac tcgaatcaag tcgcgcacca ggatgcggcc ctcgtcagag
    10441 tagcgaaatt ggcgccgggc aatgcgagcg gccaggtccc gagcataagg taagctcacg
    10501 ggcaacaggc gctcatcgat ccccagcaat ctcccgagat ggcaccaggt gtccagataa
    10561 ccttgctgct ccgcctcgga tagcggagct ccgatgcgca ataggccgtc caagaccagc
    10621 gccgaaaacg tgagcagcgt ccccgccaag tcctcctgat tgataggctt cccccaatcg
    10681 tcaagccacg ggtcgaggtg ttgcatcaac tcgacgtgac ggcgaatcaa ggcgtgcatc
    10741 aatcgcactt tcaacgtcga tcgcagccca attccgccgt ccagcagcgc tccgggctgc
    10801 atcacgttaa tcaacatttg cgcggtttcg cacagacgac gctgggtatc gtcggcgagc
    10861 cgccgggccc gaaataggac ctggacccca ttcgcggccg cgtaggacgc gggcaaccca
    10921 taacaaccga ggatgagcag cacctcggga ccataaagct gaaacagccg ttgtccgcgt
    10981 tcgagcgccc tgggatcatt cgatatgacg ggcggtaggc tttcgagcag gggacgaaaa
    11041 cgttcgtcat ccaagggcag cgaatcctgc tggaacaggc tgccgagcat ctcgccgatc
    11101 ctagccgccg agtacgacgt gatcagcggg tcgaggacag ggtcgccgat ggcgcgatag
    11161 gcatccaagg accggttgtc aggtgcggtg tcaccggaga cggtgcctct agaaatcttg
    11221 gatacgctag tctcgtgatc gttcatggtt ccaccagctc cgccggggct ctgagttggg
    11281 caatacgtct ccgtggaata acgagcggcc tcgggcacga acccatccac ctacgttccg
    11341 agcaactcgt actacccgcg ctcggtacga ctcgatgaac tacaccctcg accgtacccg
    11401 aagcaacgat cacggggttt acgcggccga gtaaacgccc gccagcgggg gcggcaggct
    11461 cgcctcgaaa cattttctgc ggaaccttca cggtggtacc gctgtgtaaa taggcatggg
    11521 agacgacgag cacgcgagcc cgcccacacc ggtacccggc acccggcaga gcgttggcat
    11581 acccgagccg ccgagccgcg atttgctgct ggcctgtaaa gcccaaaatc aggctgcctt
    11641 tcgcgtgttc gtggcgagct atcagcgcgc cgtgttcgcc ttgttatctc ggactaccgg
    11701 cccaggggcg catgttcagg atctcgcaca ggaggtcttc ctgcgcgcgt ttcgggcgtt
    11761 ccctcgattc gacgtggacg gctcggcccg agcttccacg tggctgttga cgatcgcggt
    11821 gcgtctcgcg ctgaacgcac gcaaaggctt gagccgtgcc cagcagctgt acccactcgc
    11881 ggaggcggaa gcgctgccgg ccgcgggcac cccggagagc gaacgcacgc gccgcgaatt
    11941 gggaacagca atcgcgaaag ccgcacagga gctatcgccg gagcaacggg ccatcttcgt
    12001 gctcagcgaa tttcacggca tgtccatttc cgaaattgcg aacgcggtgg agatccccga
    12061 agccaccgtc aaaacgcgct tgttcagggc tcgcgcgcag ctgcgcgaga gcttggccag
    12121 tttctggagt ttcccgaggg agcgcagtca tggagcgtaa catgtccaaa gaatgggata
    12181 gctgggaggc acaggcaccg tcgcacgact tcgtagatcg ggtcatgcac gagttacgct
    12241 tcgaaggcac accggtggtg ccagcgcgtt catggcgttc accgagatcg ctcgccatgc
    12301 tcgtcggagc ggcggccttg ctcgcgtcgt tggcactgtt ggcggcgagt ctccagcagc
    12361 gctggggaac gcactctgga agtagagaaa agccacaggc tcgctcagct tcgctgggag
    12421 atgacggcga acggcgcagc gacagcgcgg ggccaatcaa tagcggccgg ggccataacc
    12481 tggattccgg aactcggtgg aacgctcact ccgagcgagc ggctacggta gatcgtgagc
    12541 tccgcgcgag ggtgagtgaa aagctcgctc cgcgactacg agcccacggc gtcgagcgtg
    12601 atccccacac cgggctcacc attccgtcgg gtcaagctgg accagttgga aatatcactc
    12661 gcgagtacat ccagcagcgc atccaaaagg atttcatgcc gctggcgtta gcttgctacg
    12721 aggccgcgct cgcgaagcac cctgcgctcg cgggcaccct ggccatcgac ttcatgatcg
    12781 ttggagaccc gagcgtcggt ggcattatcg accaagcgaa gatcaatgcc tcgagcacgc
    12841 ttcacgacct cgagctatcc aattgcatgc tcggctcgct gcagagcatc gtctttgccg
    12901 cccccgacaa ctatggctgg gtaacggtta cttatccgtt cgtgctttct ccgggttcgg
    12961 gaagcgggga gaactgagca ggcctgtacc cttggcgcct cggactcgaa gcgacgggcc
    13021 gcgccggaac gccgctttcg tgcgagcccc gatgtgacgc ttcgtcctcg atccgttgaa
    13081 gcaccgtcgt ggaggtcggt cagccccctg aactccgcga atgcctcgga tgtgattgtg
    13141 tcgaggccgt ttggtcgagg tcactcgatc ttcatgagct ccagctcgta gatcaatgct
    13201 ccccccggtt ggtgccgtcc cggtttgtcg ccgtaagcga gggcggccgg gatccagaag
    13261 cgcactctgt cacccggacg cagaaggggt aaaccctcac gtaggccggg gatcaaagtg
    13321 gaaacgctgc tgctgacggg ctgctcggac atttccgagc tctcgaacag agtgccactc
    13381 tcggtccaca cggagtgaga gaacgtgacg cgagtgtttg ggaggatcgc cgggccttgg
    13441 gggccccgtg gtttcatgaa ttcgtaggcc aagccgctgg cggtccgtgt tgccgtccga
    13501 ggcgcctgac gaagacccgt gggcagcacg ggcgcgcgac gaattcgaag taattcgact
    13561 tcgaatgtca ggtcttcggg gatgtgcggc ttctcgtcgt tcgacgcgaa agtgaggcgc
    13621 gccggaaccc aaatccgacg ctgctctcct tcggtcatac tcgcgatcgc ttccgcgagc
    13681 cccggcacca tgtatctcag agtttgaacg taaggttcgc cgcgtaagcg ggagctatcc
    13741 acgagcgtgc cagtacgctt ccagctggta tagtggaccg tcactcggtc atcgggcaat
    13801 ggattggagc ttccaccgcc tgacttcagc acacgatagg caacgcccgc cgcgctaacg
    13861 ctcgcgtctc ccggaggagc cgaaacatcc gaaggagccg aaggatcggg agcaatcgag
    13921 ccgccgtccg acgcgagcgc gcccgggggg gacggagctc cgcgcgagct cgcgctcagc
    13981 gcggatacct gcctcgaccc accgggcgtc gaatacggtt gcccgcgcga gaatgcccgc
    14041 cagcccaccc aggcaccgag caccaccgcc ggcgcgatga gtgctaggtg ccatttgcgt
    14101 atcgaactcg acttcttcat cgacgcaggg gcgggaccga cgggtgccat gttcaggggg
    14161 cgattacact gaattgttgg gcggcggtgc cattgcacgt ccactcgtga atattggtac
    14221 cgctggtggt ccctgagtta tccacgtcca tgcacttgtt ggctgccact tggggcgaga
    14281 tgcggtaccg gccgttgcca atagagtcta ccgaccagat ctgagcgtta gtgccgttgc
    14341 actcccattg atggatattg gtgccatccg cggttccgga attgtccaca tccaggcact
    14401 tctcgctgac cgaagcacgc acctcgtaca ggttcccgcc caagctccgg aaacgaaagc
    14461 gctgcgcatc cgacccgttg cactcccact ggtggagatt gacgccatcg cctcctccgc
    14521 cgttatcgat gtcgaggcac tgacccgagg tcgtggaggt cgtgatgcgg aaggtgctgt
    14581 tcggaatggg ctgcgcgccg gtgttgtcga acgccagcgt gagtgtttgg gcggcgctac
    14641 cgttgcacgt ccattgttgg atattggtgc cgtccgcggt cccgctgttg ctgacgtcga
    14701 ggcaccgatt ggcggcggtt tgcggcatca atcgatattg gccattaccg agcgcttgca
    14761 ccgcccagcg ttgcgcattc gagccattgc acgtccactg acggatattg gtgccgtccg
    14821 ccgtgcctga attgtcgaca tcgaggcact tgttgctgtt tacgttgcga atctcgtaaa
    14881 tatcgttcgc aatgttgatg aactggaaac gctgggcgtt cgtgccgttg caggtgaact
    14941 gctgcaagtt gccgccgtcg gccgtcgagc tattgaagat gtccgcgcac ttgccggtcg
    15001 tcgttcgggt tcggatttga tagatggcgt tggcgatggg cggagtgggt ccggagcgcg
    15061 cgccgaacac ggagaccgcg cccgagaccg aggcgaggta gactttgcct ttggcgatga
    15121 gtggtggact gcccttggag agccccaata catcgtctcc gggcgagcta ctatcccaga
    15181 gcagcgccaa cgtctccgcg ttgaatgcgc gtagaatcgc gggtactgac gcttggttcg
    15241 cattgccctg tgaaggcgtg gtggcccaca gaatgcctgt gcccgccgtg ctgccgttcg
    15301 cggacaaact catcgttccc ccgggcatgc ccgggggtgg cagcaccgag cccacgagag
    15361 tggccggagt gatgaacttt tgggtcgcag agttgaagcg atacgcccgg aggtagtcgt
    15421 tctctcccca gacatacata ttgatgccag cggggctgct ccaaaccacg ttatgattgt
    15481 gaatatggtg tgtgtccccg gggcgcgccg ccgagtccac ggcttggaag ctctgcggga
    15541 tctgggcatc gccggccacc atgccaccca ggctcgagct gttcagcagg tatactttgc
    15601 cttctttccc accgttcacg atcaaattcg tgccgggtag gaagcttggc cccgctgaac
    15661 cgaaatcgtc gtcggccgcg ttgagcgtgg caaagttcga aggcgtgaag aagttcgagg
    15721 tcgagttcgt gcgcgtgagc gtacggggcg cgagcttcaa gagcgtctcc ccgaagttct
    15781 tggtgccgtt gaagtcgccg ttgccggtcg tcacataaac gttaccggcg gagtccacgg
    15841 cgggtgcgct accggcttgc cagatgccac ccagggttcc gttgggcgtg gtgttgaaca
    15901 cgcccgtcga ggccaaggag gtcacatcga agcccatcag ccagccgtgc gtgggccgcg
    15961 cgtcgcagta cgcggcaaac gccacgtaga cggcgttgga ggccaaagcc aagcctgaac
    16021 gttggttgtg gaacatggga ttgaaggcga cgttggtccc atcgctgcca tcccccgttc
    16081 ccggcaccgt cgcggtgatc tgcttcgggc tcccggcgcg atcggcgccg gtggcaatgt
    16141 tcacc
//
LOCUS       DASTKU010000611        14256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1458343, whole genome shotgun sequence.
ACCESSION   DASTKU010000611 DASTKU010000000
VERSION     DASTKU010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14256
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458343"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VFQ61_21370"
     CDS             <1..693
                     /locus_tag="VFQ61_21370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957067.1"
                     /translation="AMTDAGKALAEYTLMGITNTDWEDLAVGPGPSDGSFIYVADIGD
                     NAARTGGTARTEIQVLRVAEPDISTTQALAQKTLSGVEVLRFTYPDKAHDAEALMVDP
                     VSHDLLIATKESDGTTVIFRAPGDTPVDTPTKLEKVTSVQVGASGMDTQVSAGDASPS
                     GDRFALRTYGNVLMWPRAATFVATFSAMPKTLPGDDGPQSEGLTFSADGTSLISSGER
                     ARTIYESKSACK"
     gene            769..1689
                     /locus_tag="VFQ61_21375"
     CDS             769..1689
                     /locus_tag="VFQ61_21375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957068.1"
                     /translation="MFRRAFWIGSVLAALGPACGGKEASELFHSQSGGGKASTGGTGA
                     NAAQGGNAGRATSSTWGGSAASGSAVAGSAGAGENARGGQSASEDGTETSGGVASNAG
                     NAAGGVGSSATGGSSVTFGGGSGVASGGASQAGSSTLGAQGGAAAGGTTAIAGGTPSG
                     GTGSGGAANGGSTSGGVDSQGGQGGGTSSSGGKSAGGGGGALGGSSSTVDCAALLMQM
                     ATELAAAQECSNARDREPCAATVMNECGCSVVVDDASSVSSRNYVKLREEYGRGCKSM
                     CQDLPCPAPRMQRCMSERGAAQGRCASREP"
     gene            complement(1834..4017)
                     /locus_tag="VFQ61_21380"
     CDS             complement(1834..4017)
                     /locus_tag="VFQ61_21380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957069.1"
                     /translation="MHLGSRRALSCVGLAVFMLACSSDETASRPRGGGTGGARPGGLG
                     GSGSIPIPGNGGTGPVLPGGGQPSLPNGPVSGGVVPDDDPSNPNITHPTCDVGTCADF
                     PEQPIMGEGVPANAPTLFGADPAAFTAGTLCVLEPQLSNGSTPGTMIPANWVRPRFKF
                     NAMGANLFEIRIKNAAQKHELVAYTTKTTWYMPKEIWAGNVANSIAAGSGAANNGAGA
                     PFEVTIRGVNTASPGTPVGVKGEFHVAPVVATGSMVFWTVNSAVVTPESSKLLGFAVG
                     DEGVAETVTLKNVRWTGQIGENGATLRGYYDKPKVAGFTDGQVRCIGCHTSTPDGLSV
                     IFTDDWPWPKAVASVSDMGGAVGAVPTWLTPGARALLKLPWLGTQTMSKAHWKQGDRI
                     MVTSYGVKTKNGKVRDKPWYGLDYDNNDRIQTHQLAWFDLEANITVNDTVALNAEEYG
                     EKQDARNNAVTAAQGKAWGLIATGDTQSAVSPSFKNDGTAIVYTVTDYTPDGHPDYTG
                     KVADIKTVSFGANRAGGTGQPVKGASEANYLEYYPSYSADDKFIAFTRAPAVSSTSPD
                     GPYYNRFGEIMVVPSEGGTATPLAANAPNSCAGDNVQRGIINSWPKWSPDVFKAHGKT
                     YYFAIFSSARKYGDEFSTQFQLPTSTASAFQGLTDSSQLWLAAIVVDESGNITTYPAQ
                     YIWNQNRLAAAGGAATNVQFSNLTPAWDPFVLPPITIAPPGEPPR"
     gene            complement(4893..6344)
                     /locus_tag="VFQ61_21385"
     CDS             complement(4893..6344)
                     /locus_tag="VFQ61_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET9957070.1"
                     /translation="MKKFILPLLAWALVCLGVLVSPTPAYADTPEPAAAAQALEKRVA
                     DLEAYINNGARADEATSKVAAPGPGHNGFMMICAALVLFMTLPGLALFYGGLVRSKNV
                     LSIMAQCMGIAGLVTLLWWAVGYSFTFAPGSPFLGNLSMAFLSGVAAAPNTAYAAWVS
                     QSTFSMYQLMFAIITPALIVGAIAERIKFSALMLFVAGWMLIVYFPLAHMVWGADGLM
                     NGVWNANAKIKAIDFAGGTVVHMSSGWSALVLALIVGKRKGFGSENFAPHSLVLTMVG
                     TGMLWVGWYGFNAGSAVAADGIAALAFMTTTIAAAVGGAAWAAIEWVTRGKPTVLGFC
                     SGIVGGLVVITPAAGFVTASGAIIIGVLAGIVPFFACTKLKTALGYDDALDTFGVHAV
                     GGTLGALLTGFLAKADANPNLNTNLKDIVGSTLWVEQLKAMGLTLALSIAGTLVLGYV
                     LKAAIGLRPTAEDEESGLDITGHGERGYHMNEV"
     gene            6617..8395
                     /locus_tag="VFQ61_21390"
     CDS             6617..8395
                     /locus_tag="VFQ61_21390"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957071.1"
                     /translation="MSTRTSALPDGPATLTSDIRRSVATEPAELISADPDSANPEDQL
                     DLSIWDPREAVRAAANRELHTELVREAAARVGKISLFTTLALLFYWIATWLLTPSAVA
                     GAAGLVDSARKVFVGCILLSLAMFAVTRLPRLKATHVTDLGYVYLFLLALLLSLLRHL
                     GTSSVPDLIRQISPVCIPILAFGALIPAAPTRALAVAVSAALMDPLAMFMLHANHRYS
                     ARELPFVFASPLIAALVAQRISREVYRLNEGIAKAREVGSYKLVERLGTGGMAEVWRA
                     HHRMLARPAAVKLIRPQVLIEHGPADSQRLLRLFTREVRATASLSSPHTIQVYDFGIT
                     REGAFYYVMELLTGLDLQSLVERYGPQPSERVAHLLSQACLSLREAHERSFVHRDIKP
                     ANMFTCVVGGEHDFVKVLDFGLVLDRHPTTEELEDEKRFVGTPTVMAPEMVRFQAPVD
                     ARADLYALGCVGYWLLTGKRVFDAETRHDMLVMHAHQKPIPPSRRVGSPVHPGLETLI
                     MACLEKNPNRRPQSARELNDALNGLMFTHAWTEERAALWWRQHGLNALRAGAVPSSRS
                     EPAPSGDDLEDTPNSNPLESVQKEAP"
     gene            complement(8421..10187)
                     /locus_tag="VFQ61_21395"
     CDS             complement(8421..10187)
                     /locus_tag="VFQ61_21395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957072.1"
                     /translation="MQRIDFYRLERPIQERFIASARGTAAPAPLALEREPLQRGALLA
                     GVVSGLTALAALGLASLGFGNLQSSLALHPSTLLVLYALLIATSAAAAVVAYRVQATR
                     NTLPFAPGIYLFPSGVIDTRGPEFRVRKLSDVARVTSSAKHIQLLFQDGTRFQFKVPS
                     GTQPSDVERKLNDLRSRLSTESGPPSARELAIYDPLCDNGFKNPFSPTEPMRPPRTRL
                     LALSAAVGAVVGAALGAGLWKVRNVLGEDALYRAARETHTRDGYAAYVERGGSNPDVR
                     EVLLPRANLAAIVERGSLEEIERFAEAQKGSRVEVEAQAALYKALTSELERAKQEQSF
                     AALKAFRDRYAKHTTIQGELSRAFDERLNSTLAEVERRAHPKPDVLTAFRRLLAYSVD
                     HTPRVEVRFRRRIGESVAKTETLIEKSLAFGGRSSLPAQYFQGNYVETREAEVAKQFI
                     DAFSKSLPPGVLTFEHAPPLEDSPDEDPKVSTPSIVVTHRVEMSGGYLMRKPKAALTG
                     VGVLFRATIQVPGEPAYHPFKYSSWNVPDFKSMVDGKSFAEIYDEMGDKAFAKLSRKY
                     LAEVLPGIPLPGATDADHAPGG"
     gene            10776..11243
                     /locus_tag="VFQ61_21400"
     CDS             10776..11243
                     /locus_tag="VFQ61_21400"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9957073.1"
                     /translation="MLIRWNEQNPLSRFGRESNRPYDAFDELRREMNRLFYDFENGSG
                     FEDHAFPRVSFEDTGAAFELKFEVPGVKDKDLELSVQESSVTVKGQRADAVPEGYSVH
                     RKERAAQRFARSFALPAKVDAERAEAVLRLGILTLTLPKAKEAQPRQISVRSA"
     gene            11276..11635
                     /locus_tag="VFQ61_21405"
     CDS             11276..11635
                     /locus_tag="VFQ61_21405"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9957074.1"
                     /translation="MSEQAISNHRSAAVESASPARRVSPPVDIYENAEGFLIVADLPG
                     VAPDALKVEFNPPELHVQGRLSAAQGPETLYERRFELGRSVDPAGIQAHLEHGVLRIE
                     LKKAAALRPRRIEVRSA"
     gene            complement(11703..12776)
                     /locus_tag="VFQ61_21410"
     CDS             complement(11703..12776)
                     /locus_tag="VFQ61_21410"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET9957075.1"
                     /translation="MSDLKEIQNLLGDKASFLLEHTSKTISKDKLHLPSKNFVDDVWA
                     ISDRNPQVLRSLQALFNNGRLAQTGYLSILPVDQGIEHSAGASFAKNPAYFDPENIVK
                     LAIEGGCNAVASTYGVLASVARKYAHKIPFIVKVNHNELLTYPNKAEQIVWGTIKQAW
                     DMGAVAVGATVYFGSADASREIVEVAGLFQKAHELGMATILWCYLRNKAFKVGNVDHH
                     VSADLTGQANHLGVTLNADIIKQKLPEVNGGFKALNTGESSYGKLDERIYSELTSEHP
                     IDLCRYQVANCYMGRAGLINSGGASGKNDFAEAVATAVINKRAGGMGLISGRKAFQRP
                     MAEGAKLLQAIQDVYACKEVTIA"
     gene            13042..13725
                     /gene="deoC"
                     /locus_tag="VFQ61_21415"
     CDS             13042..13725
                     /gene="deoC"
                     /locus_tag="VFQ61_21415"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET9957076.1"
                     /translation="MPQPTAREVASLIDHALLAPHVSEPEIEAGLALARRHSVASVCI
                     VPWALPRAVQFLKGSPVRASTTIGFPHGAVPSEIKRHEAEWALVQGAEELDMVVNLGL
                     VTSGRYLEVERDIAAVLEPVRAAGKKLKVIFENAYLNQAQKAELCRISNDLRVDWVKT
                     STGFGPSGATLEDLKFMREHAHPSVQVKASAGVRNLATVLAFRPYVTRIGTSHTAAIL
                     AELEASAES"
     gene            13774..>14256
                     /locus_tag="VFQ61_21420"
     CDS             13774..>14256
                     /locus_tag="VFQ61_21420"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9957077.1"
                     /translation="MARLTGRRILITGISRGIGFETAKLFLSEGAHLLGVGKDPERLE
                     RARQLLDPSGERLAVVAADLESPEATAQIVQAAERRFSGLDVLLNNAGVQIDGDTQGF
                     EDNSEKTLERSLAINLMAPYRLCLALLPLLRRGNEPRILNVSSGAGTFAALTESRIAT
                     Y"
BASE COUNT         2632 a         4403 c         4582 g         2639 t
ORIGIN      
        1 gcgatgacgg acgcgggcaa ggcactcgct gagtacaccc tgatgggcat taccaacacg
       61 gactgggaag acttggcggt aggtccgggg ccgagcgacg gctcattcat ctacgtcgcc
      121 gatatcggtg acaacgctgc acgcaccggc ggaacggcgc ggactgaaat tcaggtgctg
      181 cgcgtcgcgg aaccggacat ctcgacgacg caagcgctcg cgcagaagac gctctccggc
      241 gtggaggtgc tgcgtttcac gtatccggac aaggcgcacg atgcagaggc tttgatggtt
      301 gatccagtga gccacgacct gctgattgcg accaaagaga gcgacggcac caccgtgatt
      361 ttcagggctc cgggtgacac tcccgtggac acacccacga aactcgagaa agtgacttcc
      421 gttcaggttg gggcgagcgg catggatacc caggtttcgg ccggggacgc ctcaccttca
      481 ggggatcggt tcgcgctgcg gacttacggc aatgtcttga tgtggcctcg tgcggcaacc
      541 ttcgttgcca cattttccgc aatgccgaaa acattaccgg gtgacgatgg accgcagagt
      601 gaaggcctca cattttctgc ggatgggacc tcgttgattt cgtccgggga gcgcgcccgc
      661 acgatctacg aatcgaaatc tgcctgcaag tgagtggtcg cgagccactc ggcttactac
      721 cactgagtga tcgttcggcg cgcagcgaag ccagcattgg gagacgggat gttcagacga
      781 gctttctgga ttgggagtgt tctggcggcg ctgggccccg cttgtggcgg caaagaagcg
      841 agtgagctgt ttcactcgca atctggtggt ggcaaagcgt cgacgggcgg aactggagcg
      901 aacgccgctc agggcggcaa tgccggccgc gccacttcga gtacttgggg aggtagtgcc
      961 gcgagcggca gcgcggttgc tggcagtgcc ggagcgggtg aaaacgcacg cggaggtcaa
     1021 agtgcgtccg aagatggaac cgaaaccagt ggcggtgttg cgtcgaatgc gggcaacgcc
     1081 gccggcgggg tcggctcctc tgcgaccgga ggctcctccg taaccttcgg cggtggctct
     1141 ggcgtggcga gcggcggcgc gagtcaggct ggctcttcta cactgggagc tcagggaggc
     1201 gcggcggctg gcggaaccac agccattgct ggagggacgc cgagtggtgg tacggggagt
     1261 ggcggcgccg caaatggagg ctccacgagt ggaggtgtgg acagccaggg aggtcagggc
     1321 ggtggcacct catcttccgg cggtaagagc gcaggcggag gcggtggagc actgggcggc
     1381 agtagctcga ccgtcgattg cgccgcgctg ctgatgcaga tggcaaccga gctggcagca
     1441 gcgcaggagt gctccaacgc gcgagatcgc gagccctgcg ccgcaaccgt gatgaacgaa
     1501 tgcggctgtt cggtagtggt ggacgatgcc tcatccgtga gttcgcgcaa ctacgtcaaa
     1561 cttcgcgaag agtatggacg cggctgcaaa tccatgtgtc aggacctgcc ttgtccggcg
     1621 ccgcgcatgc agcgctgtat gagtgaacga ggggcagcgc aaggtcgctg cgcgagccgg
     1681 gagccgtagc cttcggttga gcggaccgtc gcgtgtagga tcgcgccttc ggcttattcg
     1741 ttcgagccga gagctcacgc tgcaaagtac acaaaacgca tcgagcgctc ccggggggaa
     1801 gcgctcgatg ggacgttgtg gcttgttcgt agatcagcgc ggcggttcgc cgggcggagc
     1861 gatggtgatc ggcggcagca cgaacggatc ccacgcaggc gtgagattgc tgaactgcac
     1921 gttggtggcg gctccgcccg ccgcggccaa ccggttctgg ttccagatgt actgcgcggg
     1981 gtaggtcgtg atgtttccgc tctcatccac cacgatcgcg gccagccaga gctgggacga
     2041 atccgtcaga ccctggaacg cgctggccgt agaagtcgga agctggaact gcgttgagaa
     2101 ctcgtctccg tacttacgtg ccgaggagaa gatggcaaaa tagtaagtct tgccgtgagc
     2161 cttgaacacg tcgggggacc acttgggcca gctattgatg atgccgcgct ggacgttgtc
     2221 acccgcgcag ctgttcgggg cgttggcggc caagggggtt gcagtcccgc cttcactggg
     2281 caccaccatg atctcaccga agcggttgta gtacgggccg tccggtgacg tgctgctgac
     2341 ggcgggtgcg cgcgtgaaag cgatgaactt gtcgtccgcc gagtaagacg gatagtactc
     2401 gaggtaattg gcttcggagg cgcccttcac cggttggccc gtgcctcccg cgcgattggc
     2461 cccgaacgac accgtcttga tgtccgcgac cttgcccgtg tagtccgggt gcccgtcggg
     2521 cgtgtaatcg gtcacggtgt aaacgatggc cgtgccatcg ttcttgaagc tcggtgagac
     2581 cgcgctctgg gtgtccccgg tcgcgatcag gccccaagct ttgccttggg ccgcggtgac
     2641 ggcgttattg cgcgcgtctt gcttctcacc gtattcctcg gcgttcagcg cgacggtgtc
     2701 gttgaccgtg atgttcgcct cgagatcgaa ccacgccaac tgatgcgttt gaatacgatc
     2761 gttgttgtcg tagtcgaggc cgtaccacgg cttgtcgcgc accttgccgt tcttcgtctt
     2821 tacgccgtag ctcgtgacca tgatgcgatc gccctgcttc cagtgcgcct tgctcatggt
     2881 ctgcgttcca agccagggaa gcttgagtag cgcgcgagct ccaggggtga gccaggtagg
     2941 aaccgcgccg accgctccac ccatgtccga gacggacgcg actgccttcg gccagggcca
     3001 atcatccgtg aagatcaccg agagcccgtc aggcgtggaa gtatggcagc cgatgcaacg
     3061 cacctgaccg tccgtaaagc ccgccacctt gggcttgtca tagtagccgc gcagggtcgc
     3121 gccgttctca ccgatctggc ccgtccagcg tacgttcttg agcgtgaccg tctcggctac
     3181 gccttcgtcg cccaccgcga acccgagcaa cttgctcgat tcgggggtca ccactgcgga
     3241 gttgaccgtc caaaacacca tgctgcccgt cgctacgacg ggcgcgacat gaaactcgcc
     3301 ctttacgccc accggagtgc ccggcgacgc ggtgttcacg cctcggatcg tcacctcgaa
     3361 cggagcccct gcaccgttgt tggctgcccc tgaccctgcg gcgatcgaat tggctacgtt
     3421 cccggcccag atttccttgg gcatatacca agtggttttg gtggtgtacg cgacgagctc
     3481 atgcttttgg gcggcgttct tgatgcgaat ctcgaatagg ttcgctccca tagcgttgaa
     3541 cttgaagcgc gggcgcaccc agttcgccgg aatcatggtg cccggagtcg agccgttcga
     3601 gagctggggc tcgagcacgc acagggtacc tgctgtaaag gcagctgggt ccgcaccgaa
     3661 caacgtcggg gcattggcgg gcacaccctc acccataatc ggctgctcgg ggaaatccgc
     3721 gcacgttccc acgtcgcagg tggggtgagt gatgttgggg ttgctgggat cgtcatcggg
     3781 gacgacgccg ccgctcaccg gaccgttcgg caaggagggt tggccgccac ccggcagtac
     3841 tggtcctgtc ccaccgttgc cgggaattgg aatactgccc gacccaccca gaccgcccgg
     3901 gcgagcacca cccgtaccac cgccacgcgg tcgtgacgcc gtctcgtcac tcgagcaagc
     3961 cagcatgaac acggccaaac caacgcaaga aagagctcga cgcgaaccca agtgcatgac
     4021 gtcacctacc tcaattctgc gaaccaacgg gactttaacc gattcgcccg gccacgcggc
     4081 gaaccacgct cgaccaaggc gatcctaagc tttcgaactt tcgctcgcga accgaagtcc
     4141 tgcgcgagcg aacctgactg cattgaatca actactgcat ggaatcgact gagaggcagc
     4201 tgcaacccct tcgtggcgag aacgaggggc gaaagtgctg cggccagcgt caggcgtagc
     4261 acgtcttgca actcgtggag cgatcacttc ggtaggggca tggcttcgag cgggtcagcg
     4321 cttcgcgcag gtcatgtcct cgcagtgagg gctcgggcaa gctgcggcag ttgtcgagaa
     4381 agatccctgc caatcagcat gctcgaccgc tttccgtcct cgtgctcatc cgcgtggcgc
     4441 gcagaccgtg cgttgatgcg acgttgtaac tggcgtgcag tctcagcagt agaatgcgcc
     4501 tcgtgcggga gcgttgcgcg ccagcacccg aacacgctgt acacctgtgg tgatgattta
     4561 cgggcgcagc gccatcgaag gctgtgcggg aggccccctt ggtcagattg ggggcttggt
     4621 gtccatcgtg gaattttgcg aattgtttgg atctggcaac cgtctcttat gggcggcggc
     4681 tccgctccaa acgtgatcgc cgtgttcgcg gcactcgatg ggtgcgtggg tcattgctct
     4741 aattagctcg ctaacctgcc aaaaacgcgc acgcggtggc gcatagaggg catccgcagc
     4801 cgcccacaaa aaacgcgcgc cccgcgtccg ctccgaagag cgacacgcgg ggcgaacatc
     4861 cagctcggac ccgggaacgg ggtgggtcgc gttcagacct cgttcatgtg gtagccacgc
     4921 tcgccgtggc cggtgatgtc gaggccactc tcctcgtcct ccgccgtcgg acggagccca
     4981 attgcagcct tgagcacgta accgagtacc agggttccgg cgatcgagag cgcgagggtc
     5041 aagcccatcg ccttgagctg ctccacccac agcgtggaac ctacgatgtc cttcaggttg
     5101 gtgttcaggt tcgggtttgc gtctgctttg gcgaggaagc cagtgagtag tgcgccgagg
     5161 gtaccgccca ccgcgtgcac gccgaaggtg tcgagcgcgt cgtcgtaacc gagcgccgtc
     5221 ttgagcttgg tgcaagcgaa gaacggcacg atgccggcga gcacaccgat gatgatggcg
     5281 ccgctcgcag tgacgaaccc agccgccggc gtgatcacga ccaagccgcc cacgatgccg
     5341 gagcagaaac cgagcacggt cggcttgccg cgggtgaccc actcgatcgc tgcccaagcg
     5401 gcgccgccta ctgccgccgc gatggtggtg gtcatgaatg cgagggccgc gatgccatcg
     5461 gcggcaaccg cgctaccggc gttgaagccg taccagccga cccacagcat gcccgtgccc
     5521 accatggtga gcaccaagct gtgcggcgcg aagttctcgg atccaaatcc cttgcgctta
     5581 cccacgatca acgcgagcac gagcgccgac cagcccgaag acatgtgcac cacggttccg
     5641 cccgcgaagt cgatggcctt gatcttcgcg ttcgcattcc agacgccatt catcaggccg
     5701 tccgcgcccc acaccatgtg cgcgaggggg aagtaaacga tcagcatcca gcctgcgacg
     5761 aacagcatca gcgcgctgaa cttgatgcgc tccgcgatgg cacccacgat caaggccggg
     5821 gtaatgatcg cgaacatgag ctgatacatc gagaacgtgc tctgcgagac ccacgcggcg
     5881 tatgccgtgt tcggcgccgc agccacgccg gacaggaatg ccatgctcag gttacccaag
     5941 aatgggctac cgggggcgaa ggtgaagcta taaccgacgg cccaccacag cagcgtgacc
     6001 aagccggcga tgcccatgca ttgcgccata atcgagagca cgttcttgct ccgcaccaag
     6061 ccgccgtaga agagcgccag gcccggcaag gtcatgaaca acaccagagc ggcgcagatc
     6121 atcatgaagc cgttgtggcc aggtcccgga gccgcgacct tcgaagtcgc ttcgtccgct
     6181 cgcgcgccgt tgttgatgta cgcttccaaa tcggccacgc gcttttcgag cgcttgagcc
     6241 gcagccgccg gttccggagt atctgcatag gcaggggtcg ggctgaccag aacacccagg
     6301 cacacgagtg cccaggccaa aagcggcaag atgaacttct tcatagcgga gaatccttcc
     6361 atagaaacgc gtagatcgaa cgcggggccg gagtctgttc cccggtagag cacggctgcc
     6421 ggtgggtgca ggcgctagct ccgagcgagg actaccctgg cccggcgaat gtttcggtcg
     6481 cgtatccctg aagcttccga tttcttcgcc ggaaacaggc gatttccccg aacgagaact
     6541 ggcgcagcgc gtagcagact cactcaggac gagctcggcc gcgagaatac agtcattttc
     6601 gggttaagct tgccgcgtgt cgacccgaac cagcgcgctc ccggatgggc cggcaaccct
     6661 gacgagtgac ataaggcgct ctgtggctac agaacctgca gagttgatca gcgcagatcc
     6721 ggattctgca aatcccgagg atcagctcga tctcagcatc tgggatccac gcgaggcggt
     6781 gcgggcggcg gccaatcgcg agctccatac cgagctggta cgcgaggcgg cggcacgcgt
     6841 cggaaagatt tctttattca ccacgctagc gctgctcttt tattggatcg cgacgtggtt
     6901 actcacgccc tcggccgtcg caggagcggc cgggttggtg gactccgcgc gcaaggtctt
     6961 cgtcggctgc atcctactga gcttggccat gtttgccgtg actcggttgc cgcgcttgaa
     7021 ggcgacccac gtaacggacc tcgggtacgt ctatctgttc ttgctggcct tactgctctc
     7081 gttgctccgg cacttgggca cctcatccgt ccccgatctg attcggcaga tctcgcccgt
     7141 ttgtattccg atcttggcct tcggggcgtt gattccggcg gccccaacgc gggcgctggc
     7201 ggtcgcggtg agcgcggcgc tgatggatcc attggccatg ttcatgctgc acgcgaatca
     7261 tcgctacagt gcgcgtgagc tacccttcgt gttcgcctcc ccattgattg cggcgctggt
     7321 cgcacagcgc atttcacgcg aggtctatcg gctgaacgag gggatcgcca aggcgcggga
     7381 agtgggcagc tacaagctcg tggagcgctt gggaacaggc ggcatggccg aggtatggcg
     7441 ggcgcatcac cgcatgttgg cgcgccccgc ggcggtaaag ctgattcgcc cgcaagtgct
     7501 gatcgagcac ggccccgccg attctcaacg cttgctacgc ttgttcacgc gcgaggtgcg
     7561 cgccacggct tccctgagtt ccccgcacac catccaagtt tatgacttcg ggatcacgcg
     7621 tgagggggcc ttttattacg tgatggagct gctcacgggg ctcgatctgc agagcttggt
     7681 ggaacgctac ggtccccagc cctccgagcg cgtggcccac ctcttgtccc aggcttgtct
     7741 gtcgcttcga gaggctcacg agcggagttt cgtgcatcgc gatatcaagc ccgccaacat
     7801 gttcacttgc gtggtgggcg gggagcacga cttcgtgaag gtgctcgact ttggcttggt
     7861 gctcgatcgt caccccacca ccgaggaact cgaggacgag aagcgtttcg tcggaacccc
     7921 gacggtgatg gcgcccgaga tggtgcggtt ccaagctccg gtggacgcgc gagcggatct
     7981 ttatgcgctc ggttgtgtgg ggtattggct gctgaccggc aagcgcgtct tcgacgcgga
     8041 gacgcgccac gacatgttgg tcatgcacgc ccaccagaag ccgattccgc cctcacgtcg
     8101 cgtcggcagt ccagtgcacc ccgggctcga gacattgatc atggcgtgtc tcgagaagaa
     8161 cccgaaccga cgcccgcaga gcgcccgtga gctgaacgac gctttgaacg ggctgatgtt
     8221 tacccatgct tggacggaag agcgggcggc gctgtggtgg cgccagcacg gcttgaacgc
     8281 gcttcgcgcg ggcgcagtgc cctcctcccg gtcggagccg gctccgagcg gcgacgatct
     8341 cgaggacacg ccgaactcga acccgctcga gagcgtgcag aaggaagcgc cgtaaacgga
     8401 ggcgctggtg cgcctcgcga tcacccgccc ggcgcgtgat cggcatcagt cgcgcctggc
     8461 agcgggatgc ctggcaacac ttccgccaag tacttgcggg agagcttggc gaaagccttg
     8521 tcgcccatct catcgtagat ctcggcgaag ctcttgccgt ccaccatgct cttgaaatcg
     8581 ggcacgttcc acgacgagta cttgaacggg tgatacgccg gctctccagg gacttggatc
     8641 gtcgcgcgga acagcactcc gacgcccgtg agcgcagcct tgggcttgcg catcaagtaa
     8701 cccccggaca tttccacacg gtgggtgacg acgatgctcg gggtgctgac cttgggatcc
     8761 tcgtcgggcg agtcttcgag gggtggcgcg tgctcgaacg tgagcactcc tgggggcaag
     8821 ctcttgctga aggcgtcgat gaactgcttg gccacctcgg cttcgcgcgt ctcgacgtaa
     8881 ttgccttgga agtactgcgc cgggagcgag ctccggccac cgaaagccag actcttctcg
     8941 atcaatgtct ccgtcttggc tacggattct ccgatccgcc tgcggaagcg cacctcgacc
     9001 cgcggtgtat gatccaccga gtaggcgagt aggcggcgaa aggccgtcag cacatcgggc
     9061 ttcgggtgag cccggcgctc tacctctgcc agcgtcgagt tgagacgctc gtcgaacgcg
     9121 cggctcaact cgccttgaat cgtggtgtgc ttggcatacc ggtctcggaa tgccttgagc
     9181 gctgcgaacg actgctcttg cttggcccgc tcgagctccg aggtcagagc cttgtaaagc
     9241 gcggcctggg cttccacttc gacgcgcgag cctttttggg cttccgcgaa gcgctcgatt
     9301 tcctcgagcg aaccgcgctc cacgatggca gccagattgg ctcgtggcag caacacctca
     9361 cgaacgtcgg ggttgctgcc gccgcgctcg acgtaagcgg cgtagccgtc ccgggtgtgc
     9421 gtctcgcggg cggcgcgata cagcgcatcc tcgcccaaca cgttgcggac cttccacagc
     9481 ccggccccga gagccgcgcc gacgaccgcc cctaccgcgg cgctcagggc caataatcgc
     9541 gtgcgcgggg ggcgcatcgg ctcggtggga gagaatggat ttttgaaccc gttgtcacac
     9601 aaggggtcgt agatggcgag ttctcgcgca ctcggcggac cagattctgt cgacagccgc
     9661 gagcgcaagt cattcagttt gcgttcgacg tcggagggct gcgtgccgct cgggactttg
     9721 aattggaacc gggtcccatc ctgaaaaagc agctgaatgt gcttggcgga gctcgtcacc
     9781 cgagcgacat ccgacagctt tcgaacccgg aactcggggc cgcgcgtatc aatcacgccc
     9841 gaagggaaga ggtaaatgcc cggcgcgaag ggcaacgtgt tccgagtagc ctgcacgcgg
     9901 taagcgacga ctgcggcggc cgcgctcgtc gcgatcaata gagcgtacag aaccagcagc
     9961 gtgctcggat gcagcgccaa cgaactctgc agattgccga agccgaggct ggccaacccg
    10021 agcgcagcca gcgccgtgag gcccgaaacg acccccgcca acaatgcacc acgctggagt
    10081 ggttcgcgct ctagcgcgag cggcgccggg gcagcggtgc cacgggccga cgcgatgaag
    10141 cgttcttgga tgggtcgttc caggcgatag aagtcgattc tctgcatgct ggtcggcccg
    10201 cgaatatgcc gaccttaaaa caaaaacggt cagaagcgaa atgccgtgta ggtaggaccg
    10261 cgtgcgggtg tgtcacgcca gctgcggtgc tcgcgccgag ctccgcgtgc gcctgccagt
    10321 cggcggctcg aaatgtctac attctggaca atttgccgag ggggtcggcg gctcaagtgc
    10381 cagaagcgga aagcttcgct gtcagcagta tcgacgccga ccgcgagcgc gctcagtgac
    10441 ctgcgggtcc cccccttgcc aacgacgcag gcggtgcaca ctttccggaa agccaaggac
    10501 gccggacggt ttcaatccag acctgacaga tgcttggggt tgctccccaa gcactcttct
    10561 cgcgtcgcgc gatgtaccca tctcgcggcg tgcgggcgag ttctcgcggc tcgaacatag
    10621 ggcttcgaga acgaactcgg accaggtgga cacgaagccc cgcgagattg gctaggacga
    10681 aaacggtgag gtggagaggc gagcacgaaa cgctcgggat ctatccgtgc gccgtgattt
    10741 tcgtcccacc ccctagaccc aaaggagatt cagtcatgct cattcgatgg aacgaacaga
    10801 atccgctcag ccgcttcggt cgcgaatcga accgccccta cgacgcgttc gacgagctgc
    10861 gccgagagat gaaccgtctt ttctatgact tcgagaacgg ttcgggcttc gaagatcacg
    10921 ccttcccgcg cgtcagcttc gaagacaccg gcgccgcctt cgagctcaaa ttcgaggtgc
    10981 ccggagtgaa ggacaaagat ctggagttga gcgtgcaaga gagcagcgta accgtcaaag
    11041 gccaacgcgc tgatgcggtt cccgagggat actcggtgca ccgcaaggaa cgcgccgccc
    11101 agcgctttgc gcgcagcttc gcgctgccgg ccaaagtgga tgccgaacgg gctgaagccg
    11161 tactgcgcct cggcattctg accctcactc tacccaaagc caaagaagct caaccacgcc
    11221 aaatcagcgt gcggagcgca tgaaccctcg aacgaaagag aaaggagaat cgaagatgtc
    11281 cgaacaagct atttcgaacc atcgtagtgc cgcagtcgag agcgcgagcc ccgcacgccg
    11341 cgtgtccccg ccggtggaca tttacgagaa cgccgagggt ttcctgatcg tcgccgattt
    11401 gccgggagtg gcgccggacg ccctcaaggt ggaattcaat cctcccgaac ttcacgtgca
    11461 aggccgcttg agcgcggcgc aaggaccgga gacgctttac gagcgtcgct tcgagctcgg
    11521 cagatcggtc gatccggccg ggatccaggc gcacttggag catggcgtgc ttcggatcga
    11581 attgaagaaa gcggccgctc tgcggccccg acgcatcgaa gtgcggagcg cgtgaccccc
    11641 gagtctcgaa cagacggcgg aagatagccg tgcgcgagca gactacttcc gccgtcccgg
    11701 gttcacgcaa tcgtgacttc tttgcaagcg tagacgtctt gaatggcttg caacaacttt
    11761 gcgccttccg ccatcgggcg ctggaaagcc ttgcggccgc tgattaggcc catcccgccc
    11821 gcgcgcttgt tgatcaccgc cgtggcgacc gcttccgcaa agtcgttctt gccggaggcg
    11881 ccgccgctgt tgatgagacc cgctcgtccc atgtagcaat tcgccacctg gtagcgacac
    11941 aggtcgatgg gatgctcgct cgtgagctcc gagtaaatgc gctcgtccaa cttgccatag
    12001 gacgattcgc cggtgttcaa ggctttgaag ccgccgttca cttccggcaa cttttgcttg
    12061 atgatgtccg cgttcagcgt gacgccgagg tgattggctt gaccggtcaa gtccgcggac
    12121 acgtggtggt cgacgttacc gaccttgaac gccttgttgc gcaaatagca ccacaggatc
    12181 gtcgccatac cgagctcgtg cgccttctgg aagaggcccg cgacctcgac gatctctcgt
    12241 gatgcgtccg ccgaaccgaa gtacacggtc gcgccgacgg ccaccgctcc catgtcccaa
    12301 gcttgcttga tcgtgcccca cacgatctgc tcggccttgt tcgggtacgt gagcagctcg
    12361 ttgtggttga cctttacgat gaacgggatc ttgtgcgcgt acttgcgagc tacggaggcg
    12421 agcacaccat aggtgctcgc gaccgcgttg cagccgccct cgattgcgag cttgacgata
    12481 ttctcggggt cgaaataggc ggggttcttg gcgaacgatg cccccgcgga gtgctcgatg
    12541 ccttgatcca cgggcaatat ggacaaatag cccgtttgcg cgagccggcc gttgttgaac
    12601 agagcctgaa ggctacgcag cacttgtgga ttgcggtcgg aaatagccca aacgtcatcc
    12661 acgaagttct tcgacgggag gtgaagtttg tccttcgaga tggtcttgga ggtgtgctcg
    12721 agaaggaatg atgccttgtc gccgagtaga ttttggatct ctttcaggtc actcattgcg
    12781 cagttactcc gtcctcattg cgtatgcggc cgtgttgctt gcggccgaaa gccgccggcc
    12841 ctgtcgttcc gccaagcgtg aacgccgagt ttacaccctt cgattcgaaa cgcgagacac
    12901 agccccggag gaaggggctc gcggggaaaa gttcgtcgtg gcacccgtgt ccaagcttcg
    12961 acgcgggccc ttgttgcgag cgcgcgctcg ttatatccgg agggggcccg ccatacgtct
    13021 accgctatgc ttcccactgt catgccgcaa cccaccgcac gagaagtcgc atcgctcatc
    13081 gaccacgccc tgcttgcccc gcacgtcagt gagccggaga tagaggccgg cctcgcgttg
    13141 gcgcggcgcc actcggtcgc gagcgtttgc atcgtgccct gggcattgcc ccgagcggtg
    13201 cagtttctga aggggagccc agtgcgcgcg agcaccacga ttggttttcc gcatggcgcc
    13261 gtgccgagcg aaatcaagcg ccacgaagcc gagtgggcgc tagttcaagg cgccgaggag
    13321 ctcgatatgg tcgtcaacct cggcctggtg acgagcgggc gctatctcga ggtggagcgg
    13381 gacatcgccg ccgtgctcga accggtgcgc gccgcgggaa agaagctcaa ggtcatcttc
    13441 gagaatgcct acctcaacca agcgcagaaa gccgagctct gccggatctc gaatgacctg
    13501 cgggtggact gggtcaagac ctcgacgggg tttggcccga gcggggctac gctcgaggat
    13561 ttgaagttca tgcgcgagca cgcgcacccg tctgttcagg tcaaagcttc tgccggcgtc
    13621 cgcaacctcg ccaccgtgct cgccttccgc ccgtacgtga cgcgaatcgg caccagccat
    13681 acggcggcaa tcctggccga gctcgaggcg agcgccgaat cctgagccgc attgttccga
    13741 acgtcgggga tttgcgctat gggaccacga accatggctc gcttgactgg gcgtagaatc
    13801 ctcatcactg gcatctctcg tggcatcggc ttcgaaaccg ctaagctgtt tctgagtgaa
    13861 ggtgcccacc tcctcggagt aggcaaggac cccgagcggc tcgagcgagc gcgccaactc
    13921 ttggatccgt cgggcgaacg tctcgcggtc gtggcggcgg atctggagtc gcccgaagcg
    13981 actgcccaga ttgtgcaggc ggcggagcgc cgattctcgg gtctcgacgt gctgctgaac
    14041 aacgccggcg tgcagatcga cggcgatacc caaggcttcg aggacaacag cgagaagact
    14101 ctggagcgtt cgctggccat caatttgatg gctccatatc ggctctgcct ggcgctgtta
    14161 cctctgctgc ggcggggcaa cgaaccgcgc atcctcaacg tcagctcggg cgcggggacg
    14221 ttcgcggcgc tgacggagag ccgcatcgcc acgtat
//
LOCUS       DASTKU010000612        16316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_564746, whole genome shotgun sequence.
ACCESSION   DASTKU010000612 DASTKU010000000
VERSION     DASTKU010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16316
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564746"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFQ61_21425"
     CDS             complement(<1..617)
                     /locus_tag="VFQ61_21425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957078.1"
                     /translation="MQLKPSTLLAYSALVCSAALGVIACGGEEANNPPAGSGGSTATG
                     AGGASTAGAGGSITGGASAGGATNSSGGATASTGGKSTTAAGGTTSVQSGGASSKGGA
                     SASGGSTAVGGTSGIGGSAAGAGGMSASGGTSSTGGANSGGSAGSAGSGGGTGACASV
                     KSIASSTCTVGESNCFSFFVASRARMFALAQAFNGSTKGWGGDFR"
     gene            complement(854..2011)
                     /locus_tag="VFQ61_21430"
     CDS             complement(854..2011)
                     /locus_tag="VFQ61_21430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957079.1"
                     /translation="MSTVKSDKIAGFASVGVVLVVLGSGVGCSSKDSTASNPATGGAA
                     SGAPSSQGGSAQGGSSAQGGSSAQGGSSAQGGGSAQGGATGSGGAATGTGGAANGNAV
                     VGSFAVLLNPAADENPAYTSVIGKVYSGEYPTDVIETAVANSGSCTVYQYSRHSCIAP
                     ACTTSQVCVEMDVCRDRPELVSVGAVSLEGFGAAALKLSAINNNYQYAGDIAYPGFSE
                     GATIKLTAAGAHYPAFSVSVAGVAPVELTKESYMLKYGQPLSVEWKAGSSSVNARVAV
                     LLNISKHGGSAGYLKCDVADSGSLTIAGEWISTLMDLGVAGFPQLTFTRSTKAQAPVS
                     GGVITLEVDALAIPKLEIDGYCSCFDSSDCGSCSDSTKSVCDSVKKLCHAP"
     gene            3223..4524
                     /locus_tag="VFQ61_21435"
     CDS             3223..4524
                     /locus_tag="VFQ61_21435"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET9957080.1"
                     /translation="MVSVTRRGVLGAESGVRLRSRGAGLLSVFGPALLTCGFVACGTA
                     GDQEAVAHVEQAINVCGASVASNLVVDGIPAYAQCDASSNSAIYSNNGVDTATSAASS
                     EWKRTQYNGGYQCTELLHRYWLFKWSVSWIPTGNAGTWCDATPPANSGIVKTTSPVHG
                     DAIVFAPGSCGADSVTGHVALIDTVDSAGSKVTFVEQNRAGRRSSNMSCASCFLHVVA
                     NDGTASGSGGSSAGGASTGTGGSSSGGRPGMATGGRSGATTGGGSNNSGGTSLEAGGA
                     GAGGRPRNEGGSRSEGGATGSSLAGSPPGSAQGGSSLGTSTVHTGGASSTNAGSTTGS
                     VASGGSLPVAGGASNTDAFVSPPQGGGSTSEERPGTSASGGAAGSSASSTGTTPAQDE
                     SGCNFRVGSAPSTWAWLLSLVALLAAQQRSSRRRARNRPEV"
     gene            complement(4551..5564)
                     /locus_tag="VFQ61_21440"
     CDS             complement(4551..5564)
                     /locus_tag="VFQ61_21440"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamide-nucleotide
                     adenylyltransferase/Nudix hydroxylase"
                     /protein_id="HET9957081.1"
                     /translation="MEQSFDAAILVGRFQPFHAGHARLLERALAVAPRVVIVLGSAFH
                     ARTAKNPFTWQERAEMIRDTLALEARARVTFAPVRDWYDDLKWSQAVEEAVLERVSPG
                     SRCALVGCFKDQSSYYLRHFPRWALITVEPLSGLEAQHVRAEWFDATDPQRYLSALDA
                     RLPVSVRRFADAFSRTPEFEQLREEHRAIVQSRKTYGRGPFVTVDAVVTVNDRVLLVQ
                     RGRAPGKDLWALPGGFLEPEESLVSGALRELREETGLELAESACADVAVFAHPARSLR
                     GRIITHAHYFPLGELPELPVVKGADDAAAAQWLPVSEVADAEQRFFDDHFHVLRRFLK
                     GLN"
     gene            complement(5610..7319)
                     /locus_tag="VFQ61_21445"
     CDS             complement(5610..7319)
                     /locus_tag="VFQ61_21445"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET9957082.1"
                     /translation="MTPLRITVAQLNPTVGDFEGNLERMRRAAEQAQQDGANLVVFPE
                     LSLTGYYPGDLLDEPGFARAIEHGLERVAAASRETPGLYWVVGAPTLREGAGKRWHNS
                     LLVYHEGALRLRYAKQLLPTYNIFDERRHFEPGPDVAKVLRIGDAQVGFLICEDGWND
                     EQRDYAVNPFLRLADAAPDIVISINASPSNIGKREQRHALFRAASERHALPILYVNQV
                     GGQDQLVYDGASFAVTPSQGVVFEAARFVEHVVTLNFESGTFSGHAGRQLEPVSPTGL
                     PLMEFYRRQIVLGLRDYARRCGFQRVVVGSSGGIDSALTLALAVEALGAQNVTAVTMP
                     SSYSSAGSVTDSQLLCNNLGVTLFEHPIRELVLKYAQGFGATFGGELRGVALENLQAR
                     ARGTILMEFSNTFGHLLLTTGNKSEISVGYCTLYGDTNGGLGLIGDLYKTEVFELSRY
                     LNHEYGREVIPNAILEKEPSAELAPNQRDSDSLPPYAILDPILKHLIEGDRLGADERS
                     SVEQAYTALIGAETGRALVQRIRQLIARNEYKRRQAAPILRVRARAFGSGRQMPIAAR
                     HEF"
     gene            complement(7316..8590)
                     /gene="pncB"
                     /locus_tag="VFQ61_21450"
     CDS             complement(7316..8590)
                     /gene="pncB"
                     /locus_tag="VFQ61_21450"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF003704.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET9957083.1"
                     /translation="MNAQRTPVVRSLLENDLYKFTMWQSLLHSHPAAQAEYSFVCRNQ
                     PAFPLAELQEEVEREIEHLCSLSFKLDELEYLRGLRFLKSDFVDFLSLFRFQRRFLDV
                     HREGERLVIRAQGPLVHVMGFEIFVLYIVNELYFRRLPAGPEIQQEARARLKKKITWL
                     QEQRPQLTRDPNYPFIFFDFGLRRRYSANWHAEVVESLARGAPQHFRGTSNVYFAKHF
                     GLTPIGTMAHEYLQAYQAFGSRLRDFQTAALEGWVQEFRGDLGIALTDVVGMDAFLRD
                     FDLYFAKLFDGLRHDSGDPIEWGEKAIAHYRSLRVDTASKRLVFSDGLNLPRAQQIHQ
                     HFSGRIQTSFGIGTDLSNDTPHRALNIVMKLLSCNGQPVAKLSDSPGKTLCEDITFLS
                     YLRQVFEHPAPLTAGPSGPAPSKEPALTQVQS"
     gene            complement(8633..9493)
                     /locus_tag="VFQ61_21455"
     CDS             complement(8633..9493)
                     /locus_tag="VFQ61_21455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957084.1"
                     /translation="MQNHLLLIDPQNDFCDLPASAYSELNAGAGPALPVAGAHQDMLR
                     VAALIEQAGAGLTDITITLDSHQHFDIGHPSFWRHADGTAVSPFTQVTADEVRAGRIV
                     PRRAELVARVLQYLEALESRGRYRHMVWPVHCEIGTWGHNVHASVRRAYNQWEEAYDA
                     SVAKVLKGKNPYTEHYSAVQAEVPDPGDSETDVNRELLTSLEAADRVYIAGEASSHCV
                     KSTVEHIVQFLSPTQRKNLALLSDCMSPVSGFEAQARSFLDTMRQAGVRLLGAAEAIA
                     ELRRERGARV"
     gene            9707..10384
                     /locus_tag="VFQ61_21460"
     CDS             9707..10384
                     /locus_tag="VFQ61_21460"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET9957085.1"
                     /translation="MNPSIICTVDVVLLTLHQNALHVALVRREREPYANAYALPGGYI
                     HAQEDADAEEAATRVLRTKAGIASPYLEQLGTFSGRGRDPRGWSISVSYYALVPESVF
                     GGERPADLKLVPVDRARGLPFDHQYVIETAVARVRSKSSYSSLPVYLCSEPFTLPRLQ
                     AVYEALLGEPINKVSFRRKMEELDMLEPVQGAQETGRANRPAQLYRLKARFKRQLSTT
                     TRALNPD"
     gene            complement(10406..13708)
                     /locus_tag="VFQ61_21465"
     CDS             complement(10406..13708)
                     /locus_tag="VFQ61_21465"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HET9957086.1"
                     /translation="MTDAQNESEEVDQSAQALRSNAPPSSCGYRVESDHHPRWWRAGY
                     FGELELTNVSGERAHEFEVFADLGGTAPRHCVHSDCEAVEGGYLFSAPPGIKRGGIRR
                     GETHHILFSSREPYAGVKPYVLSINGVRCDQVPPEVTLAASQTFVPTGGVLTLSADAR
                     DDVAIRKVVFERDGVVLGVDTEAPYSWEIPARAGGASDTRHTYTATAYDPSGNRTLSN
                     GVRVLLGGGQKFFGTAPDNTADYTHLLRYFNQLTPGNAGKWGSVEATRNQMDWAKLDT
                     AYAFARDNGIRFKLHTLIWGQQQPGWMDGLTQAEQLQEIEQWIAAVAQRYPDVEMVDV
                     VNEPLHAVPSYSAALGGAGSTGWDWVIRAFELARKYFPRAELLLNDYNVEALESFTTD
                     YLKVIDVLNQRGLIDGIGLQAHFLERAELPIVATNLDRLAATGLPLYVSELDVNFAND
                     ARQAQRMRELFTLFWQHPSVVGVTHWGHLQGSTWRTDAYLVRSDGSTRPALDWINCFR
                     AGGTDCSVPEYVPEPRIGDATSITLQAEDYDAAQGVLALGDVVAYTDDGDWQSFSRVR
                     FDSNWDTLSVTYAKGNPEPGSVSVHLGSLESEPLATVTLAPTGGWGTSKTISVPISPI
                     LATNEQDLFVRYNGGPGVGNLDRIRLGAPAGSGPNRVSNSDFEVDTSGWFTWDGTLST
                     SEQYAVSGKRSLRLSGRSGNGPAATSLTGLVGPGKTYDVSLWTTISGADAAEVNITQK
                     ITCDGTESYNWLVNPTTVQAGQWTELRGSLTIPECNLGEVLIYAEGPAAAVDLFLDHV
                     SVRAQQSSNLIENGGFEAGIAGWSTWNGTASQSTVRAHSGTSSLLIGNRTDNAPAATN
                     LTSVVKAGGNYQVSFWVAIGGAESANVNLTKKIVCNGQDATYSWIANPTAVADGAWVE
                     LQGTLDVPNCELGEVTVYAEGPAAGVDLYVDDVKVFAPTISNLLADGSFESSTDGWFT
                     WGGGNRATTADRAHGGTKSLLLSGRTGNSPLAKSLLSLVKPGTSYQVSVWTTIAGAAS
                     ANVNLTQKIGCDGSDSYSWLANPTAVTEGEWVKLSGTLSVPSCSLTDVLVYAEGPAAG
                     VDVYLDDAVVSP"
     gene            complement(14207..15070)
                     /locus_tag="VFQ61_21470"
     CDS             complement(14207..15070)
                     /locus_tag="VFQ61_21470"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9957087.1"
                     /translation="MPSTIAVRPRDLVSPPLLYGTAWKEERTESLVLQALQAGFRGLD
                     TANQRKHYFEAAVGQALAIAEQRGIVRREQVFLQTKFTFRAGQDQRLPYDSAARVADQ
                     VRQSFVSSLEHLRTDYLDSYLLHGPSRRNGLGPEDLEAWHAIEQLRASGKARYIGVSN
                     VSVEQLRLLAEQSHILPSFVQNRCYAATGWDRECRAYCEQTGIVYQGFSLLTANRREL
                     QRPIVARIAKRLRVTPAQVVFAFAQRVGMLPLTGTTNPLHMKQDLESTELLFEPADLA
                     ALSELDTPTSA"
     gene            complement(15088..15519)
                     /locus_tag="VFQ61_21475"
     CDS             complement(15088..15519)
                     /locus_tag="VFQ61_21475"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9957088.1"
                     /translation="MDPSPKPRNSPEINPSPLEIVQSFLAALSNGERQNAQAFYAENV
                     EQVEFPNRLVGAGARRGLPELREASERGARAVSSERYVIRNAVEMGERVAVELEWSAV
                     LNVPLGSIPAGGTLRAACAMFFTVREGRIVRQHNYDCFDPF"
     gene            15557..16090
                     /locus_tag="VFQ61_21480"
     CDS             15557..16090
                     /locus_tag="VFQ61_21480"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9957089.1"
                     /translation="MKRPRAAATSPRETRPRPAAPLLEPDQLSLTQLALFVGQAADEQ
                     LLEQLRSAGFQDIKVSHGYVVQHLLGGPRAIGELAALLGVTQQAASKTAAEMVRAGYA
                     ELIPGEDARVRQLRLSERGAACVERARSARKRLEARLSQHVGSESYEDARRTLGGLLD
                     ALGGSEAVRKRRVRMPR"
     gene            complement(16121..>16316)
                     /locus_tag="VFQ61_21485"
     CDS             complement(16121..>16316)
                     /locus_tag="VFQ61_21485"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HET9957090.1"
                     /translation="SLGEPRKFLIRARAGGSSRAFSLGSGDLLVMGGTCQRTFQHGVP
                     KASRAGPRMAIMFRPAWDID"
BASE COUNT         3094 a         5205 c         4991 g         3026 t
ORIGIN      
        1 taccggaagt caccacccca acctttggtg ctgccgttga aggcctgcgc cagcgcgaac
       61 atgcgcgcgc ggctggccac gaaaaagctg aagcagttcg attcgcccac ggtgcatgtg
      121 gaggacgcga tcgacttcac cgaagcacac gcgcccgtgc ctccacccga gccagccgaa
      181 ccagctgagc cgcctgagtt cgccccgccg gtgctgctcg tgccgcccga ggccgacatt
      241 cctccggctc ctgccgcgct gccgccgatg cccgaagttc ctcccactgc cgtgcttccg
      301 ccgctcgccg acgctccacc cttagacgag gcaccaccag attggacact cgtagtaccg
      361 ccggcggcag tagtggattt gccgcccgta ctcgccgtcg caccgccgct cgagttcgtg
      421 gcccctcccg cgctcgcgcc tccggtgatg ctaccacccg cacctgcagt actggctccg
      481 cccgcaccgg tcgctgtact cccgcccgat ccagcaggcg gattgttggc ttcctcaccg
      541 ccacaggcga tgactcccag agcggcggag caaacgaggg ctgagtaggc gagaagggtc
      601 gagggtttca attgcatgat gtatcgaggc cgcgggtagg agatggatat tgcctctcac
      661 cgaaggattt cggcgccgga ggcgaactcg aactcggaaa gaacacggaa gaatccagaa
      721 gtctgacagc tgcgatcggg cgcgcgttag aggtgccgtt acgagagctg acgcgagcac
      781 attgggttgc tcggggaaaa gcgcgcgaga agcgccgagc gatcatgtcc ggcggaataa
      841 cgagggccgg cgctcacgga gcgtgacaca gcttctttac ggagtcacaa accgacttgg
      901 tactgtccga gcaggacccg caatccgagc tgtcgaagca cgaacaatag ccgtcgattt
      961 ccagcttggg aatcgccagc gcatccacct cgagagtgat caccccgccg ctcaccggcg
     1021 cctgtgcctt ggtgctgcgc gtgaaggtca gctgaggaaa gccagccacc ccgagatcca
     1081 tcagggtgct gatccactcc ccggcaatcg tcagcgagcc cgagtccgcg acgtcgcact
     1141 tcagatagcc ggcgctgccg ccgtgcttgc tgatattgag caacacagcc acccgcgcgt
     1201 tcaccgacga gctccccgcc ttccactcga cgctcagcgg ttggccgtac ttaagcatgt
     1261 atgattcttt cgtcagctcg accggggcca ctcccgcgac ggacacgctg aacgccggat
     1321 agtgcgcgcc cgcggccgtc agcttgatcg tcgccccctc gctgaagccc ggataagcga
     1381 tgtcccccgc gtattgatag ttgttgttga tggcggacag cttgagcgcc gcggcgccga
     1441 acccctccag cgaaaccgcg cccacgctga ccaactcggg gcgatcacga cagacatcca
     1501 tctccacgca gacctgcgag gtagtgcaag ccggcgcaat gcagctgtgc cgggaatact
     1561 gatagacagt gcacgacccg ctgttcgcga ccgcagtctc gatcacgtcc gtcggatact
     1621 caccggaata gacctttccg atcaccgacg tataggccgg gttctcgtcc gcggctggat
     1681 tcagcagcac cgcgaagcta cccaccaccg cgttgccgtt cgcagcgccc ccagttcccg
     1741 tcgcggctcc gcccgagcca gtcgcgccgc cttgagcact gccgccgcct tgagcactgc
     1801 tgccgccttg cgcactgctg ccgccttgag cactgctgcc gccttgagca ctgccgcctt
     1861 gagagctcgg cgcaccggac gcggctccac ccgtcgccgg attcgaggcg gtggagtcct
     1921 tggacgaaca gcccacgccg gaccccagaa cgaccagcac gacgcccacc gacgcaaagc
     1981 ccgcgatctt gtcgcttttt actgtgctca tcccacatcc aataagaaag gttcggatct
     2041 agagaacgtt tggagaaagt ctggttgtag aagttggccc gaggaatggt ggtctgatgt
     2101 ggctcgcgac gcggctcgcg acatcatccc aattagtcgc cactcccgct gcaaacctgc
     2161 catcgaactt cacacgcatc tcacttttat cagtctctga tgactctagt gctcggcacg
     2221 ccggtgaacg gatcactgcc ggaagattat tacggtttgt ttcagaactt gcccgacccg
     2281 gccctcgaac cgtgcgggca gcgccaatgc tgccttcctc gcaaggggta ggacacccac
     2341 ccccggtgag ggccccgaac ccaccggttg ccgcgcgccc gaagagccga ccgtcatggt
     2401 gatggcccga gaggccgaga caagcttgct ctcatcgggt cgagtgctca cccttacggg
     2461 cggtggagta agggagccgc atgctccggt cgacacgtca ttcgagacag ccagacgcgc
     2521 gcgggggaaa ccggtcgagc cgcgctcgtc ggttcgctcg gagctgcgct tctggccgcg
     2581 tgcgcagcct ccccggtttc taatacgggc atgccgccag gaccaccaga gccaacggaa
     2641 cgagcgtcgc atgccagcgg cgttccgaac ccgactctcg aagccaagac cgagtaagcc
     2701 acgatgcttg gacgacgccg gctccgcgtg gtgggctcgc gcacgagagt ggttacatcg
     2761 attgaatcgt ccaagtcacg cgcttaaaac tcagcccttc gagctccgct ccagaatcga
     2821 tgttgcctgt tgcggctggc gcggtaccaa acgaagcaag gcgacgtatt ccggctgcgc
     2881 caatacggat ttggacgctt gtcgcgctcg ttccaatttg ccgccaaaca aactgcgctt
     2941 cgaaacgggc catcttccgt caaactgaga ggacctaccg ctcgccaagg ggcgcaccgc
     3001 cccgagcgcc attatgaatg taggtgcgtg gttcctcggt tggagtcgac cgaggcccga
     3061 tataccgtga ccgcgctgag aaagtaagat tctcgaacta ttcggaattc cgctgagtcc
     3121 cattctggcg tgcgattcga gttcggcgaa ttttctgttc tacaccgaag atggctctca
     3181 gtcttgaacg ccgagctcgt ggacgtccat aatcgatgct ccatggtatc ggtcacacgt
     3241 cgtggtgttc ttggtgctga gtcgggggtg cggcttcgct cacgcggcgc gggattgctt
     3301 tccgtgtttg gtcccgcgct gctcacctgc ggattcgtgg catgcgggac ggcaggagac
     3361 caagaagcgg tggctcacgt ggaacaagcc atcaacgtgt gcggcgcgag cgtggcgagt
     3421 aacctggtgg tcgacggaat ccccgcctat gcgcagtgcg atgcttcgag caattctgct
     3481 atctattcca acaacggggt ggatacggcg acgagcgcgg cgtcgagcga gtggaaacgc
     3541 actcaatata acggtggata ccaatgcacg gagctgctgc atcgttattg gctattcaag
     3601 tggagtgtca gttggattcc gaccggcaac gctggcacct ggtgtgacgc cacgccgccc
     3661 gccaacagcg gcatcgtgaa gacgacgagt cccgtgcacg gtgatgccat cgtgtttgcg
     3721 ccagggagct gtggtgcgga cagcgtgacc ggccacgttg ccctcataga caccgtagac
     3781 agcgccggat ccaaggtcac gttcgtcgag cagaatcggg ccggacgccg tagttcgaat
     3841 atgagctgcg cgagttgctt cttgcacgtc gtcgcgaatg acggcaccgc gtccggctct
     3901 ggtggcagta gcgctggtgg cgcaagtact ggcaccgggg gcagttcgag cggtggacgc
     3961 ccgggtatgg cgacgggagg tcggagcggc gccacaactg gagggggttc gaacaactcc
     4021 ggcgggacat cactcgaggc gggcggcgcg ggcgcaggtg ggaggccgcg aaacgagggc
     4081 ggctctagga gcgagggcgg agcgacgggc tcatcgttgg cgggatctcc gccaggcagc
     4141 gcccagggcg gctcctcatt gggaacctcg accgtccaca cgggcggcgc gagttcgacc
     4201 aacgccgggt cgacgaccgg ctctgttgca tccggaggca gcctgccggt agcgggcggc
     4261 gcatcgaata ccgacgcttt cgtgtctccc cctcagggtg gtgggtcgac gagcgaagag
     4321 cgcccaggca cgtccgcatc cgggggggcc gcggggtcca gcgccagctc gaccggcaca
     4381 actccggcgc aggatgagtc ggggtgcaac ttccgggttg ggagcgcacc gtcgacctgg
     4441 gcgtggctct tgtcgctggt tgcgttactt gctgctcagc agaggtcgag ccgacgacgg
     4501 gcacgcaatc gtccggaggt ttgagtgccg tagctcgccc tccgagttgg ttagttcaga
     4561 cctttcagga agcgtcgcag gacgtgaaaa tggtcgtcga agaagcgctg ctcggcgtcg
     4621 gctacctcgc tgacgggcag ccattgcgcg gccgcggcgt catcggcgcc tttgaccacg
     4681 ggcagctctg gtaactcgcc cagcgggaaa tagtgagcgt gggtgatgat gcggcccctc
     4741 agactgcgcg cagggtgcgc gaacacggcc acgtcagcgc acgcgctctc cgccaactcg
     4801 agaccggttt cttcgcgcag ctcacgcagc gcaccgctca cgagtgattc ctcgggttcc
     4861 aagaagcccc ccgggagcgc ccagagatct ttgcccggcg cacgcccacg ctggaccaac
     4921 agtacccgat cattcacggt cacgaccgcg tctaccgtaa cgaatggccc ccggccgtag
     4981 gtctttcgcg actgaacgat ggctcggtgt tcctcgcgca gctgttcgaa ctccggagta
     5041 cgagaaaacg cgtctgcgaa gcgtcgaacg ctgacgggca gtcgcgcatc gagcgcgctc
     5101 aagtatcgtt gcgggtcggt tgcatcgaac cattctgcgc gcacgtgctg cgcctccaag
     5161 cccgacaacg gctccaccgt gatgagcgcc cacctcggga agtgacgcag ataatagctc
     5221 gactgatcct tgaaacagcc cacgagcgcg cagcgcgacc ccggagacac tcgttcgagc
     5281 acggcttcct ctacggcttg cgaccatttc agatcgtcat accagtccct gactggagcg
     5341 aacgtcacgc gagcgcgcgc ctccaaggcc agagtgtccc gaatcatctc ggcgcgctct
     5401 tgccaggtga acggattctt cgcggtgcgg gcatgaaaag ccgagcccag cacgatcacg
     5461 acgcggggcg ccaccgccaa cgcccgctcg agcagtcgtg catggccggc atgaaaaggt
     5521 tgaaagcgcc ctaccaaaat ggcggcgtcg aatgattgtt ccatgggctg ctcctgtccc
     5581 caagctccgt gtcgataaca actcgtgatt tagaactcgt gccttgccgc gatcggcatc
     5641 tgcctgccac tgccgaaagc gcgcgcccgc acgcgcaaga tgggcgccgc ctgacgtcgc
     5701 ttgtactcgt tgcgcgcgat tagctgacgg atgcgctgca cgagcgctct tcccgtctcg
     5761 gcgccgatta gcgccgtgta cgcctgttcg acgctggaac gctcgtcggc gccgaggcga
     5821 tcgccttcga tcaggtgctt cagaattggg tctaggattg cgtacggcgg taaactatcc
     5881 gagtcccgct ggttgggtgc gagctcggcc gatggctcct tctccaagat tgcattcggg
     5941 atgacctctc gaccgtactc gtgattcaaa tagcgagaca gctcgaagac ttcggtcttg
     6001 tagagatcgc caatcaagcc gagcccacca ttggtgtcac cgtagagcgt gcaatagcct
     6061 acggagattt cgctcttatt gcccgtcgtc agcagcaagt gcccaaaggt gttggagaac
     6121 tccatcagaa tggtaccgcg cgcccgcgcc tgtagattct cgagtgccac tccgcgcagc
     6181 tccccaccga aggtggcgcc gaagccctgt gcatacttca acaccagctc acgaatcggg
     6241 tgttcgaata gcgtaacacc gaggttgtta cacaagagct gggagtcagt cacgctgccc
     6301 gcgctcgagt aactcgaagg catggtcacc gccgtaacgt tctgcgcgcc gagcgcctcc
     6361 accgccaggg ccagggtcaa agcgctgtcg ataccgcccg agctcccgac gaccacccgc
     6421 tgaaagccgc aacgtcgcgc gtagtcacgt agaccgagca cgatttggcg tcgatagaac
     6481 tccatcaagg gcaggcccgt cggcgacacg ggctcgagct gccgcccggc gtgcccagaa
     6541 aacgtgccgg attcgaagtt cagcgtcacg acgtgttcta cgaagcgcgc cgcctcgaac
     6601 actacccctt gggagggagt gaccgcgaag gaagccccgt catagaccag ctggtcttgc
     6661 ccgcctactt ggttgacgta cagaattggc agcgcatgac gctcactcgc agcccgaaac
     6721 agggcatgac gctgttcgcg ctttccaata ttggaagggc tcgcgttgat cgagatgacg
     6781 atatcgggcg ccgcgtcggc caagcgcagg aatggattta cggcgtagtc gcgttgctcg
     6841 tcgttccagc cgtcttcgca aatcaaaaag ccgacctgcg cgtccccgat ccggagaacc
     6901 ttggccacgt ccggtccggg ctcgaaatgg cggcgctcgt cgaagatgtt gtaagtcggt
     6961 aacagctgtt tggcgtatcg gagccgcagc gccccttcat ggtagacgag caggctgttg
     7021 tgccaacgtt tccccgcccc ttcgcgcaac gtcggcgcgc ccaccaccca atacaagccg
     7081 ggcgtttcac gcgaggcggc cgcgacacgt tccaagccat gctcgatcgc acgcgcgaag
     7141 cccggctcat cgagtaaatc acctgggtag tagccagtga gagacagctc cggaaagacg
     7201 acgagattgg cgccgtcttg ctgagcttgc tcggccgcgc gtcgcatacg ctcgaggttt
     7261 ccctcgaagt ctccaacggt gggattgagc tgagctacgg tgatgcgtag aggtgtcatg
     7321 actggacctg agtcagcgcg ggctctttgg acggtgcagg cccggaaggg ccggccgtga
     7381 gaggtgcggg gtgctcgaac acttgccgca agtaggagag gaacgtaatg tcctcgcaca
     7441 atgtcttgcc aggcgaatcg gacagcttgg ccacgggctg tccattgcac gacagcaact
     7501 tcatgacgat gttgagcgca cggtgcggcg tgtcgttcga gagatcggtg ccgatgccaa
     7561 agctggtttg gatgcgcccg gaaaaatgct gatgaatctg ctgggcgcgc ggcaagttga
     7621 ggccgtccga aaagaccagg cgcttgctgg cggtgtcgac gcgcagacta cggtagtgcg
     7681 caatcgcctt ctctccccac tcgatgggat cccccgagtc gtgccgcagg ccatcgaaca
     7741 gcttcgcgaa atagaggtcg aaatcgcgta agaatgcatc catgcccacg acgtcggtca
     7801 gcgcaattcc gaggtcacct cgaaactcct ggacccaccc ctcgagcgcc gcggtctgga
     7861 agtcacgcag acgtgagccg aaggcttgat aggcttgcag gtactcatgg gccatcgtac
     7921 caatcggggt cagcccgaag tgcttggcga aatagacatt cgaggtgccc cggaaatgct
     7981 gtggggcgcc gcgcgccaag gactccacca cttccgcgtg ccaattcgcg gagtagcgac
     8041 ggcgcagacc gaagtcgaag aagatgaacg gatagttcgg atcgcgcgtc aattgaggac
     8101 gctgttcctg aagccaggtg atcttcttct tgagacgcgc tcgcgcttct tgttgaatct
     8161 ccggccctgc gggcaggcgc cgaaagtaga gttcgttcac gatgtacagc acgaagattt
     8221 cgaagcccat cacgtgaacg agcgggccct gggcccgaat gaccaagcgt tcgccctcgc
     8281 gatgaacgtc caagaatctg cgctgaaacc ggaacaacga caggaagtcg acgaaatcac
     8341 tctttagaaa gcgcaggcca cgcagatact cgagctcatc caatttgaag gataggctac
     8401 atagatgctc gatctcgcgc tctacttcct cttggagctc ggccagtgga aatgccggtt
     8461 gattgcgaca gacgaatgaa tactccgctt gcgcggcggg atgactatgc agcagcgact
     8521 gccacatggt gaatttgtag agatcgttct cgagcaagct ccgaacgaca ggggtacgtt
     8581 gcgcgttcat gactcagact cccgtactgt ttccaagctc tggcgccgga ttctagactc
     8641 gcgcgccgcg ctcgcgccgc agttctgcga tggcctcggc cgcaccgagc agtcttacgc
     8701 cagcctgtcg catggtgtcg agaaatgatc gagcctgcgc ttcgaagccc gacacggggc
     8761 tcatacaatc actcagcaag gccaagtttt tacgctgcgt cggagatagg aattgcacga
     8821 tgtgctctac cgtggacttc acgcagtggc tgctcgcctc cccggcgata taaacgcgat
     8881 cggcagcctc cagggaggtg agcagctcgc ggttcacgtc cgtttccgag tcgcccggat
     8941 cgggtacctc ggcttgcacg gcgctgtagt gctccgtgta cgggttcttg cccttcagca
     9001 ccttggcgac gctggcatcg taagcctcct cccactggtt gtatgccctg cgtaccgagg
     9061 cgtgaacgtt gtgaccccaa gtcccaatct cacagtgcac gggccagacc atgtgtcgat
     9121 agcgcccccg agactctagc gcctccaaat attggaggac gcgcgccacg agctccgcgc
     9181 gacgtggaac gattcggccg gctcgcacct catcggccgt cacctgagtg aagggagaga
     9241 ccgccgttcc atctgcatgg cgccaaaatg acggatgacc gatgtcgaaa tgctggtgag
     9301 agtcgagcgt tatcgttatg tccgtcagcc ctgctcccgc ctgctcgatg agtgcagcca
     9361 cgcgcagcat atcttggtgc gcacccgcga ccggcagagc cggaccggcc ccagcgttga
     9421 gctcggagta ggcggacgcg ggcagatcgc agaagtcgtt ctgcggatcg atcaggagaa
     9481 ggtgattttg catgacgggc tcgttggttt gctgtcgaac gagtcatagc ctagcccagt
     9541 aagtttcatc gcgcaactta ctgctgacgt ttgctgatcc ccgatatttt accgggcatt
     9601 cgccaactgg tgtcgccaca gggtacctgc agccgggtgg ctttttatca acattgtact
     9661 aagagagctc ccggctccct tctcgtttcc cgactgcaac cgcaccatga acccctctat
     9721 tatctgcacg gtcgacgtcg tgttgctgac tttgcatcaa aacgcattgc acgttgcgct
     9781 cgtgcggcgc gaacgtgagc cctacgcaaa cgcctacgcc ctgccagggg gttacattca
     9841 cgcgcaggaa gacgccgacg cggaggaagc cgccacgcga gtgttgcgaa ccaaagcagg
     9901 aattgcgagc ccctatctgg aacagctcgg cacgttctcc ggtcgcggcc gagatccgag
     9961 aggttggtct atctcggtga gctactacgc gctggttccg gagagtgtgt tcggcgggga
    10021 gcggcctgcc gacttgaagc tggtccccgt ggatcgcgcg cgagggctgc cgttcgacca
    10081 tcagtacgtg atcgagacgg cagtcgcgcg ggtgaggagc aagagcagct attcgtccct
    10141 cccggtatac ctgtgcagcg aacctttcac gctgccgcgt ctgcaggcgg tttacgaggc
    10201 gctgttgggt gagcccatca ataaagtgag cttccgacgc aagatggagg agctcgatat
    10261 gctcgagccc gtccaaggcg cgcaagagac tgggcgagcc aatcggcccg cccagctcta
    10321 tcgactcaaa gcgcgcttca aacgacagct ctcgaccacg acgcgggcat tgaacccgga
    10381 ctagcggtcc gcgagctgct tgcgctcagg ggctcaccac tgcgtcgtcg aggtatacgt
    10441 ctactcccgc ggccggtcct tcagcgtaca cgagcacgtc cgtgaggctg cagctcggca
    10501 cactcaacgt gccgctaagc ttgacccact ccccctcggt gaccgccgta ggattcgcga
    10561 gccagctata cgagtcggag ccatcgcagc caatcttctg agtcagattg acgtttgcgc
    10621 tcgccgcccc agcaatcgtc gtccagacgc tcacctgata gctcgtgcca ggcttgacca
    10681 ggctgagtag agatttcgcg agcgggctat ttccggtgcg ccctgagagc aataagctct
    10741 tggttccgcc gtgggcgcgg tccgcggtgg tggctcgatt gcctccgccc caggtgaacc
    10801 atccgtcggt cgaagattcg aaacttccgt ccgcgaggag attcgaaatg gtaggagcga
    10861 acaccttcac gtcatccacg tagagatcta cgcccgcggc tggcccctcc gcgtagacag
    10921 tgacctcgcc gagctcgcaa ttcggcacat ccagggttcc ttgaagttcg acccacgctc
    10981 cgtccgcaac ggccgtggga ttggcaatcc acgagtaggt ggcatcttgc ccgttgcaca
    11041 cgatcttttt ggtcaagttg acgttggcgc tttcggcccc gccaattgca acccagaagc
    11101 tgacctgata gttgccgccc gccttcacga cactggtgag gttggtggcc gcgggggcgt
    11161 tgtccgtgcg attgccgatc aacaagctgc tcgtgccgct gtgggcgcgc accgtcgatt
    11221 gcgaggcggt gccattccac gtggaccagc cggcgatgcc ggcctcgaaa ccaccgttct
    11281 cgatcagatt cgaggattgc tgcgcgcgca ccgagacgtg gtcgaggaac aagtccaccg
    11341 cggccgcagg gccctccgca taaatcagca cctcgcccaa gttgcactcg ggaatggtga
    11401 gcgaacctct gagctcggtc cattgtcccg cttgcacggt cgtggggttc accagccagt
    11461 tgtaggattc cgtgccgtca cacgtgatct tctgcgtaat attgacctcc gccgcatccg
    11521 ccccggaaat cgtcgtccat aggctcacgt cgtaggtctt cccgggcccg acgagcccgg
    11581 tgaggctggt cgcggcaggg ccgttgccgc ttcggccgct cagacgcagg ctgcgcttgc
    11641 cgctcaccgc gtattgttcg ctcgtggaca gggtgccgtc ccaagtaaac caaccgctcg
    11701 tgtccacctc gaaatcactg ttgctcaccc gattgggtcc ggaaccggca ggggcgccca
    11761 agcggatgcg atcgagattg ccgacgcccg gtccaccgtt ataacgcacg aagaggtcct
    11821 gctcattggt tgccagaatt ggcgagatgg gcaccgagat ggtcttactc gtgccccacc
    11881 ctccggtcgg ggcaagtgtc accgttgcca gcggttcgct ctccaggctc ccgaggtgca
    11941 cggacacgga accgggctcc ggattgcctt tcgcgtaggt cacggaaagc gtgtcccaat
    12001 tcgaatcgaa acggacgcga ctgaagctct gccaatcccc gtcatccgtg taggccacga
    12061 catcgcctaa cgcgagcact ccctgagcgg cgtcatagtc ttcggcttgc agcgtgatgc
    12121 tcgtagcgtc cccaatccgg ggctccggaa catactcggg taccgagcaa tcggttccac
    12181 ccgctcggaa gcagttgatc caatcgagcg ccggtcgcgt gctcccatcc gaacggacca
    12241 ggtaggcgtc ggttcgccac gtactgccct gcaggtgccc ccaatgcgtc acgcccacca
    12301 ccgagggatg ctgccaaaat agggtgaaga gctcgcgcat gcgctgggcc tggcgcgcat
    12361 cattcgcgaa attgacgtcc aactcggaaa cgtagagagg aaggccggtc gcggccaagc
    12421 gatccagatt ggtcgctacg atgggcagct cggcgcgctc gaggaagtgc gcctgcaatc
    12481 caatgccatc gatcaagccg cgctgattca ggacgtcgat gaccttcaga tagtcggtcg
    12541 tgaaggactc gagcgcctcg acgttgtaat cgttcaacaa cagctctgcc cgcgggaaat
    12601 acttccgcgc gagctcgaaa gcgcggatca cccagtccca cccggtgctg cccgcgccgc
    12661 caagagccgc gctgtaggac ggcaccgcgt gcaagggttc gttcacgacg tccaccatct
    12721 ccacgtcggg gtagcgctgc gcaaccgctg caatccattg ctcgatctct tgtagctgct
    12781 cagcttgagt caaaccatcc atccagccgg gctgttgctg accccaaatc aaggtgtgca
    12841 gcttgaagcg aatgccgttg tcccgcgcga aggcataggc ggtatcgagc ttggcccagt
    12901 ccatctgatt ccgcgttgct tcgacgctgc cccacttgcc tgcgttgccc ggcgtcagtt
    12961 gattgaaata gcgaagcagg tgcgtgtagt ccgcggtgtt gtctggggcc gttccgaaga
    13021 acttttgccc gccgcccagg agaacacgca cgccgttact gagggttcga ttgccggagg
    13081 gatcataagc cgtggccgtg taggtgtgcc tggtatccga cgcaccgccc gccctggcgg
    13141 ggatttccca agaatatggc gcttcggtgt cgaccccgag cactacacca tcccgttcga
    13201 agacgacctt gcgaatcgca acgtcatccc gcgcgtccgc gctgagcgtg agcacgccgc
    13261 cggttggaac gaacgtctgg ctagccgcca gagtcacctc gggaggaacc tgatcacaac
    13321 gtaccccgtt gatggacagg acgtacggct ttacgccggc atagggctct cggctcgaga
    13381 acaaaatgtg atgtgtctca ccacgccgaa ttccacctct cttgatcccg ggaggagcgc
    13441 tgaagagata gccgccttcg actgcctcac aatcggagtg cacacaatga cgtggagcgg
    13501 ttcctcccag atcagcaaag acttcgaatt catgggcccg ctcgccggaa acattcgtga
    13561 gctcgagctc accgaagtaa ccggcccgcc accagcgcgg atggtggtcc gactcgacgc
    13621 ggtatccgca gctgctcggg ggcgcgttcg agcggagcgc ctgagcgctc tggtccactt
    13681 cttcgctctc gttttgcgca tcggtcacgg cattggcgca accaatcgaa cccaatagca
    13741 ctgccgtcag cagtgctccc ccagcagtcc catacgcgcg agctccgaat cgatacccgt
    13801 ggagtccata cctatgcgcc cagtccatgt gcctctccgt tgagtttgcg tgacgagttg
    13861 cccaggccgc agcccgcagt ggagcaccca ccgtttgcgg cgctcccatc gaagagtcct
    13921 tatgactgaa ttggggtact cacgtccagc acgacgcgag cctttgggtt cggtgtgctg
    13981 attatcgacg ctccctcacg cgcctggctc gaacttggcg catggcgcga acgttcgcac
    14041 ggatgtgggc ggtcacctgg gcctggacgg gcagtatggg ttcgcaatcg cgctctcgag
    14101 gcggcagcga gccattcggt gcgaagttcg tactgactct gagcgatgcg tgtgggtgaa
    14161 agacgcgcat gtgggtgcga gcacggcgcg cgctcaacgc ttgctctcac gccgatgttg
    14221 gggtgtcgag ttcgctcagc gcggccagat cggccggttc gaaaaggagc tcagtgctct
    14281 ctaggtcttg cttcatgtgc agcgggtttg tggtcccagt cagcggcagc atgccgaccc
    14341 tttgcgcgaa cgcgaatacg acttgcgcgg gggtcactcg cagtcgcttg gcaattcgag
    14401 ccacgatcgg ccgctgcaac tcgcgtcgat tcgccgtgag caacgagaag ccttggtaaa
    14461 cgataccggt ttgctcgcag taggcgcggc actcgcggtc ccaaccggtc gcggcgtagc
    14521 agcggttttg aacgaagctg ggcagaatat gcgactgctc cgccagcagc cggagctgtt
    14581 cgaccgaaac gttgctgacc ccgatgtacc gggccttgcc gctcgcccgc agctgctcga
    14641 tcgcgtgcca cgcctccaga tcttccggcc cgagcccgtt ccggcgactc ggcccgtgga
    14701 gcaggtacga atccaggtag tccgttcgga ggtgttcgag tgaactcacg aaggattgac
    14761 gcacctggtc cgcgactcgg gccgccgaat cgtagggcaa tcgctgatct tgaccggctc
    14821 gaaacgtgaa cttggtctgt aagaagacct gttcgcgccg cacgatcccg cgttgttcgg
    14881 cgattgcgag cgcctgcccg accgcagcct cgaagtagtg ttttcgttga ttggcggtgt
    14941 ccagcccgcg gaagccagct tggagtgcct gcagcaccag cgactccgtg cgttcctcct
    15001 tccaggccgt gccgtagagc aaggggggcg ataccaagtc gcgaggacgg acggcaatcg
    15061 tgctgggcat gagcgaaagc atacggctca aaaaggatcg aagcagtcgt agttgtgctg
    15121 cctaacgatg cgcccctcgc gcacggtaaa gaacatcgcg caagcggcac gcagcgtgcc
    15181 tcccgcgggg atgctgccca agggcacgtt caataccgcg ctccattcca gctcgaccgc
    15241 gacccgttcc cccatctcga ccgcgtttcg aatcacatac cgctccgacg acacggcgcg
    15301 agcgccacgt tcactcgctt cgcggagctc gggcagcccc cgccgggccc ccgctccgac
    15361 tagacgattc gggaactcca cttgttcgac gttctcggcg tagaacgcct gcgcgttttg
    15421 ccgctctccg ttggacaggg ccgccaagaa ggactgcact atctccaatg gcgaaggatt
    15481 gatttccgga gaatttcgcg gcttagggct aggatccatt gcacaaccat agttgtacaa
    15541 tctaggttgt gcaactatga agaggccccg agccgccgcg accagcccgc gcgagacgcg
    15601 ccctcgaccc gccgcgccgc tcctcgagcc cgaccaattg tcgctcactc aactcgccct
    15661 cttcgtaggc caagccgcgg acgagcagct gctcgagcaa ctgcgctccg cggggttcca
    15721 ggacatcaag gtttcacacg gctatgtcgt ccagcacctg ctcggcggcc cccgggcaat
    15781 cggcgagctg gccgcactac taggcgtgac ccagcaagcc gcttccaaaa cggccgcaga
    15841 gatggttcgg gcgggatacg cggagctgat ccccggggaa gacgcgcggg tgcgccaact
    15901 gcgactctcc gagcgtgggg ccgcctgcgt cgagcgcgcc cgtagcgcac gcaagcggct
    15961 cgaggcgcgg ctttcgcagc acgtgggcag cgaaagttac gaagacgcac ggcgtacgtt
    16021 gggcggattg ttggacgcgc tcggcggcag tgaggccgtc cgcaagcgcc gcgtccgcat
    16081 gccgcgctga gcctggctgc gcgcgaaacc ctgacttcac tcagtcgata tcccaagcgg
    16141 gacgaaacat gatggccatt cgcggaccgg cgcggctggc tttgggcaca ccgtgctgga
    16201 aggttctttg acacgtgccc cccatgacga gcaggtcccc agaacccaaa ctgaaagcgc
    16261 gcgacgaacc gcccgctctc gcgcgaatca aaaactttct cggctcgccc aaggac
//
LOCUS       DASTKU010000613         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1235433, whole genome shotgun sequence.
ACCESSION   DASTKU010000613 DASTKU010000000
VERSION     DASTKU010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235433"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1862)
                     /locus_tag="VFQ61_21490"
     CDS             complement(<1..1862)
                     /locus_tag="VFQ61_21490"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HET9957091.1"
                     /translation="MLGFAWALPLAAFACTTSGPQGEAGLGDVRGGSPNTTSSLPNST
                     SPTSSSSGGKGGAPGTTMLEGGVPAMTSGTGAGSGGIAANGGVPGGGGAGELGTGGRT
                     SDQAGQTSAGAGSGGVAPSRGGASGMQTGGTLPNGGGGTGATAGSAGFVGNWPPSATF
                     SNPVIWQDLADDEVIRVDDTYYYTASNMHYSPGAPILRSYDLVNWEYAGHAIPTLDFS
                     AKYDLTQGQAYIKGTWASTLQYRKSNKTFYWLGCIEFGKTYVYTASAVEGPWQKHPPI
                     NNCYNDAGMLVDDDDTLYVAYGNTQISVAQLGADGFTQVKTQQVYSATGMTLEGSHFL
                     KYQGNYYITLTRPADGEFVLKSSSPWGPYTIRALIDRNASPIPGGGVPHQGSLVQTQS
                     GDWYYMAFVDAYPGGRVPVLAPVTWSADGWPSVQLVNGGWAASYPYPKVPRPPRAMRP
                     LTGTQTFDGPTLGHEWEWNHNPDNTKWSFDGGLKLQTATVTTDLYRARNTLTHRILGP
                     QSTGTIQLDISGMQDGDVAGLALLRQDSAYIAVKKSGGSARIVVFNGLSMDSKWNTAS
                     TGKEAASAPLTGNTVWLRLAADIRPGSGRQGKFSYSTDGSTFTALGPGCNMNNS"
     gene            complement(2007..2429)
                     /locus_tag="VFQ61_21495"
     CDS             complement(2007..2429)
                     /locus_tag="VFQ61_21495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957092.1"
                     /translation="MRADRVVRFKTRLCELTIERPAPSLIVLKLSGHDIGELGDAPFE
                     ELEGDFASEGPQRELFIDARGGQGASIEVSGSWAQWLRRHKARLRVVHFLTASRFIQL
                     SADFVRKFAELGETMRIYTDPDVFDAELESAVDRELSR"
     gene            2906..>3341
                     /locus_tag="VFQ61_21500"
     CDS             2906..>3341
                     /locus_tag="VFQ61_21500"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HET9957093.1"
                     /translation="MLLKESFSDWSDDNASRLAAALACYTLLSIAPLVVLCVVAAGMI
                     FGQDAARGQIAGELSGVVGNEAGQAIQSIVASAKAPESGLISSVVGVVVLLFGASGVF
                     GELQSAMNTIWQVKPKPGRGIKGFIRDRFFSITMVLGVAFLLL"
BASE COUNT          588 a         1096 c         1007 g          650 t
ORIGIN      
        1 cacgagttgt tcatgttgca gcccggcccc aacgcagtga aggtgctgcc atccgtgctg
       61 taggagaact tgccttgccg gccactgcca ggtcgaatat cggccgctag acgcagccat
      121 accgtattgc ccgtgagcgg cgcgctcgcc gcttccttgc cagtgctggc cgtgttccat
      181 ttgctatcca tcgagagccc gttgaagacg acgatacgcg cactcccgcc ggacttcttg
      241 accgcgatgt aggcggagtc ttgccgcagc agggcgaggc ccgcgacgtc gccgtcctgc
      301 atgccgctga tatcgagttg aatcgtcccc gtggattgcg ggccgaggat tcgatgagtt
      361 agcgtattgc gcgcccgata gagatccgtc gtcaccgtcg cggtctgtag tttcagacca
      421 ccgtcgaacg accacttcgt gttgtcgggg ttgtggttcc actcccactc gtgccccagc
      481 gtcggtccgt cgaacgtctg cgtccccgtc aggggcctca ttgcgcgcgg cgggcgcgga
      541 accttcggat acgggtaaga cgccgcccag ccgccattca cgagttgcac gctgggccaa
      601 ccgtcggcac tccaagtgac gggcgctagc acgggcaccc tcccacccgg gtaggcatcg
      661 acgaaggcca tgtagtacca atcgccgctc tgcgtctgca ccaagctgcc ctgatgcggc
      721 accccacccc cgggaatggg gctcgcgttc cgatcgatta acgcccggat cgtgtacggc
      781 ccccaggggc tgctcgactt gagcacgaac tcgccgtccg ccggtcgcgt cagggtgatg
      841 taatagtttc cctggtactt tagaaaatga gagccctcga gcgtcattcc cgtggctgaa
      901 tagacctgtt gcgtcttcac ctgagtgaag ccgtcggccc cgagctgagc gacgctgatt
      961 tgcgtgtttc cgtaagccac atacagcgtg tcgtcgtcat ccaccaacat gcccgcgtcg
     1021 ttgtagcagt tgttgatcgg aggatgcttt tgccacggcc cctccactgc tgacgccgtg
     1081 tacacgtaag tcttgccaaa ctcgatgcaa ccaagccagt agaacgtctt gttgctcttg
     1141 cgatactgca gggtcgaggc ccacgtcccc ttgatatacg cctgtccctg cgtgaggtcg
     1201 tacttcgccg aaaaatctag ggtgggaatg gcatggcccg cgtactccca gttcaccagg
     1261 tcatacgagc gcagaatcgg cgctccgggc gaataatgca tgttggacgc tgtgtagtaa
     1321 taggtgtcgt ccacccgaat cacttcatcg tccgccaaat cttgccagat caccgggttc
     1381 gaaaaggtgg ctgaaggggg ccaattgccg acaaatccgg ccgagcccgc cgtcgctcca
     1441 gttcctccgc cgccattcgg gagagtgccg cccgtctgca ttccggaagc gccgccgcga
     1501 ctcggcgcca cgcctccgct ccccgcgcct gcgctcgttt ggccagcctg gtcactcgta
     1561 cgaccgcccg tgcccagttc acccgcccct ccgcctccgg gaacgcctcc gttggcagca
     1621 attccgccgc tcccagcgcc agtacccgag gtcatcgctg gaacgccgcc ctcgagcatc
     1681 gtggtgccag gcgcgccacc cttgccgcca gagctcgagg acgtgggtga cgtcgagttc
     1741 ggcaagctcg acgtcgtatt cggtgagccg ccgcgtacgt cccctaaccc ggcctctcct
     1801 tgcgggcccg aggtggtgca ggcaaacgcc gccagtggca aggcccacgc gaatccaagc
     1861 attgcttgtc gagaagcctg cttcacgttg agacaaagcc ttaccctgcg cccggtcgaa
     1921 agacaggggc tccgatcgcc gctcgatgac attcagccac gcagtggcgg aaaggatctt
     1981 tatccgtcgc gagttctcaa aagttgtcag cgcgagagct cacggtcgac cgcgctctcc
     2041 agctccgcgt cgaacacgtc cggatccgta tagatccgca tcgtctcgcc cagctcggca
     2101 aacttccgta cgaaatcggc gctcaattgg atgaagcgcg aggccgtcag gaagtgcacg
     2161 acccgcaacc gagccttgtg cctccgaagc cactgcgccc aactgccgct gacctcgatg
     2221 ctggcgccct gcccgcctcg tgcatcgatg aagagctccc gttgcggacc ctccgacgcg
     2281 aagtccccct ccagctcctc gaacggcgcg tcgccgagct ctccgatgtc gtgccccgaa
     2341 agcttcaata cgatcagcga tggcgcgggg cgctcaatcg tcaactcaca aagccgggtc
     2401 ttgaagcgaa cgacgcgatc ggcacgcatg actcaaccgt agcaatatgc ccgcccgagc
     2461 tcattcgcca gcgtaaaatc atggctgtgg agccaccgtg attacgggcg cgacgcgcgt
     2521 ccgtaatctt cctggcgcag tcagcgggcg gcgctagcgc gtgaaaaaac gcgctcgggc
     2581 cacccgtcat ccaccgatgg agggcgttac aacgacgggc ttgcgtggcg tggtgacgca
     2641 tgatgcgcaa ttgacctgcg ccaacggcta cacgcaacgc gcagtcgcgc ccgtctacgc
     2701 tgcgtgccgg catcaactct cgaggattct tggagatcca cgatgacgcg cggctggcac
     2761 acacgctgca gtgacacggg ccgtggaaca cacggaacgg ttgcaagggc ttggcgcagc
     2821 gtcacgggcc gaaagcactc gccccgcgcg gaacacggcg cgtcgcgatt cagcgactcc
     2881 aaagggcttc ttcgcgcgcg cttggatgct tttgaaagag tcgttctcgg actggtccga
     2941 cgacaacgcc tctcggctcg cggcggcgct ggcctgttac accttacttt ccattgcgcc
     3001 gctcgtggtg ttgtgcgtgg tggcggcggg catgatcttc gggcaggacg ccgcgcgcgg
     3061 tcagattgcg ggcgagttgt cgggggtggt aggaaacgaa gccgggcaag ccatccaatc
     3121 catcgtagct tccgcgaagg cgccggaatc tgggctcatc agttcggtag tcggtgtcgt
     3181 cgtgttgctg ttcggtgctt ccggggtgtt cggcgaactt cagtcggcca tgaatacgat
     3241 ttggcaggtg aagcccaagc ccgggcgcgg catcaaaggt ttcattcgag atcgcttctt
     3301 ctcgatcacg atggtgttgg gggtggcgtt tctcctcttg g
//
LOCUS       DASTKU010000614         3514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_342309, whole genome shotgun sequence.
ACCESSION   DASTKU010000614 DASTKU010000000
VERSION     DASTKU010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342309"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..563
                     /locus_tag="VFQ61_21505"
     CDS             333..563
                     /locus_tag="VFQ61_21505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957094.1"
                     /translation="MAKRLQIIGNSAGIIIDKPILELLKITPETELELKTDGESVIIT
                     PVRTTQSRGERLARVQSKVVRKHERTFRKLAK"
     gene            570..971
                     /locus_tag="VFQ61_21510"
     CDS             570..971
                     /locus_tag="VFQ61_21510"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HET9957095.1"
                     /translation="MTAPEFLTVEDVELLHSEQLRLFGGLDGVRDRGALESAVAVPAA
                     SFGGEFLHVGLFAMAAAYAFHIAENQPFVDGNKRTALNAALVFLGLNGWDVDDPEGAL
                     YESMIGVSARTVSKEQLAGTLERLAVPYADD"
     gene            1204..1443
                     /locus_tag="VFQ61_21515"
     CDS             1204..1443
                     /locus_tag="VFQ61_21515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957096.1"
                     /translation="MTTRAKIFQNGGSQAVRLPKSCRFPDDQQEVLVHRVGRKVVLEP
                     ADEWPQEFMQCLGAWSEGIERPKSLPLSKLRDPFE"
     gene            1440..1838
                     /locus_tag="VFQ61_21520"
     CDS             1440..1838
                     /locus_tag="VFQ61_21520"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET9957097.1"
                     /translation="MSLKFMLDTDSVSFALRGYGRVGARLVEHAPSHVCISAVTAAEL
                     RFGAERRDSRKLRRLIETFTSTVSVMPFDDECAAAFGKVAARLMEKGRRIGHYDALIA
                     AHAIALEVTLVTNNEKHFGQVEGLKLANWV"
     gene            2108..2398
                     /locus_tag="VFQ61_21525"
     CDS             2108..2398
                     /locus_tag="VFQ61_21525"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HET9957098.1"
                     /translation="MRIVWDEAKNRENQRKHGLSFQEARALLVSGVDHLEIFDEEHSE
                     LEDRFVSIGPIARGIVLVVWTERDEKTTRIISARFATKREAALYRRYMGKKK"
     gene            2395..2685
                     /locus_tag="VFQ61_21530"
     CDS             2395..2685
                     /locus_tag="VFQ61_21530"
                     /inference="COORDINATES: protein motif:HMM:NF027067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HET9957099.1"
                     /translation="MTDIPELTEKQLARAIPARVRRRLMLGQFESGDDVVALRRFVGL
                     SQSKFAEAMGISVHTLRNWEQGRRRPEGAAIALLRIAARHPRIIRENLRSAA"
     gene            2930..3160
                     /locus_tag="VFQ61_21535"
     CDS             2930..3160
                     /locus_tag="VFQ61_21535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957100.1"
                     /translation="MAKRLQIIGNSAGIIIDKPILELLRITPETELELKTDGESVIIT
                     PVRKGSTRGERLAKVQSKVVRKHERTFRKLAK"
     gene            3167..>3514
                     /locus_tag="VFQ61_21540"
     CDS             3167..>3514
                     /locus_tag="VFQ61_21540"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HET9957101.1"
                     /translation="MSAPEFLTVEEVEALHSEQLRLFGGLEGLRDRGALESAVAVPTA
                     TFGGEFLHVGLFAMAAAYAFHIAENQPFVDGNKRTALNAALVFLGINGWEVDDPDGAL
                     YDAMIGISEGTMSK"
BASE COUNT          694 a         1024 c         1152 g          644 t
ORIGIN      
        1 taccggatct tctaccgcgt tgagaacaag gtgctgtggg tagtggccgt gtggcacgga
       61 gcgcagaccg tgaagcgccc gtcacgcaag ggcggcgcct aacaaggggt tgcagctggc
      121 aaggcccgcg ttgcagcgtg ctcacgtcct cgaacgcaac ccggcgtctg cgtgcaagta
      181 gcacgcaccc cccgggccgc ggctatactg cttgatgacc gaacgaagcg ggccttgcag
      241 ctgaacccca ggtccgttag gcggacaaca gagctgttcg cgcatgtcgg cgccatcttg
      301 cacgccgttg tgaacggtgt tatgaacgaa gaatggcgaa gcgcctacag atcatcggca
      361 acagcgctgg catcatcatc gacaagccga tcctcgagct gctgaagatc acgcctgaga
      421 cggagctcga gctgaagacc gacggcgaga gcgtgatcat caccccagtg cgcacgacac
      481 agagtcgggg agagcgcttg gcgagggtcc agagcaaagt cgtgcgcaag cacgagcgaa
      541 cgttccgcaa actggcgaag tgagagctga tgaccgctcc cgagttcctg accgtggagg
      601 atgtcgagct cctgcacagc gagcagcttc gtctcttcgg agggctggat ggtgtgcgtg
      661 atcgcggcgc actcgagtca gcagtggccg tgccagcggc ctcattcggc ggcgaatttc
      721 tccacgttgg gcttttcgcg atggcggccg cgtacgcctt ccacatcgcc gagaatcagc
      781 cattcgtcga cgggaacaag cgcaccgcac tcaatgctgc gcttgtgttc cttggcctta
      841 acggatggga cgtggatgac cccgaaggcg ccttgtacga gtccatgatc ggagtctccg
      901 cgaggaccgt gtccaaagag cagctcgctg gaacgctgga gcggctcgcc gtaccgtacg
      961 cggacgactg agagccgcct aacaaagggt tgcagctgac aaggcccgcg atgctgcctg
     1021 gtcacgtggg tggaacgctg cccggcgtct gcgtgcaggt agcacgcagc ccccgggccg
     1081 cggctatact gcttggacgc ggacgaatcg ggccttgcag ctgaaccctc gctccgttgg
     1141 gccgacaagg gcttcgcacc accgtagttg acaatgtata taccaatgta tatactacgt
     1201 caaatgacta cgcgcgcgaa gatctttcag aacggaggca gccaggccgt ccggctgccg
     1261 aaatcctgcc gcttcccaga cgatcagcag gaggtgctgg ttcaccgagt cgggcgcaag
     1321 gtcgttctgg agccggcgga cgaatggccg caggagttca tgcagtgcct tggggcttgg
     1381 tcggagggga tcgagcgccc gaagtcgttg ccgctctcga agcttaggga cccgttcgaa
     1441 tgagcttgaa gttcatgctg gacacggact cggtgagctt tgcgctgcgc ggatacggtc
     1501 gcgtgggtgc gcgactggtg gagcacgccc cctcccatgt ctgcatcagc gcggtcactg
     1561 ccgcagagct gcgctttggc gccgagcggc gagactcaag gaagcttcgg cgcctgatcg
     1621 agactttcac gtcgacggtg tcggtgatgc ctttcgacga tgagtgcgca gcggcttttg
     1681 gaaaggttgc cgcgcggctc atggagaaag ggcggcggat cggacactac gacgcgctga
     1741 ttgccgcaca cgcgatcgct cttgaggtga cgctcgtgac aaacaacgag aagcactttg
     1801 gccaggtcga ggggttgaag ctcgcgaact gggtataggc agcggcccaa caaggagttg
     1861 catctgacaa ggcccgcgtt gctcccgcca acgtcagtgg aacgcggccc ttgcgcctgc
     1921 gtggtagagt accaggcgat ctcggggccg cggctgtgcc gcctgcgagg ttcgaaagcg
     1981 ggccttgcag ctgaaccctc gcttcgttag accgacacag tgagagcggc gcctgaaccc
     2041 cgcgctcaat tgcgccgcgc gactaagcca ttgaccgact aagccattgg cttataatgg
     2101 ttgcttggtg cggatcgttt gggacgaggc gaagaatcga gagaaccaga ggaagcacgg
     2161 gctttccttt caggaggcgc gagcgctcct ggtctccgga gtcgaccacc tggaaatctt
     2221 tgacgaagag cactccgagc ttgaggaccg tttcgtctcg attggtccca ttgctcgagg
     2281 catcgttctc gtggtctgga ccgagcgaga cgagaagacg acaagaatta tcagtgcccg
     2341 gtttgcgaca aagcgagaag ctgcgctgta ccggcgctac atggggaaga agaaatgacc
     2401 gacatcccag agttgactga gaagcaattg gcgcgcgcaa ttcctgcgcg cgtcaggcga
     2461 cgtctcatgc tcgggcagtt cgaatcgggc gacgacgttg tcgcactgcg tcggttcgtc
     2521 ggattgtcgc agtcgaagtt cgccgaggcg atgggcatca gcgttcatac tttgcgcaac
     2581 tgggagcagg ggcgccggcg ccctgaaggg gctgcgatcg cgctcttgcg gatcgcggcg
     2641 cgccaccctc gaatcattcg cgaaaacctg cgctccgccg cgtgacaggc gcgtcggagg
     2701 cagcggtcta acaaagggtt gcagccgaca aggcccgcgg tgcagcttgg attcgtggtg
     2761 gaacgcagcc cggcgtctgc gtgcaagtag cacgcagccc ccgtcccgcg gtcatactgc
     2821 tggggaacgt acgaagcggt ccttgcagct gaaccctcgc ttcgttaggc gggcaacaga
     2881 gccttcgcgt aggtgtagcg cggtgttatg aaccgtgtta tgaacacgaa tggccaagcg
     2941 cctccagatc atcggcaaca gcgcgggcat catcatcgac aagcccatcc tcgagcttct
     3001 ccggatcacg ccggagacgg agctcgagtt gaagaccgat ggtgagagcg tgatcatcac
     3061 tccggtgcgc aaggggtcga cgcgcggcga gcgcttggcc aaggtccaga gcaaggtagt
     3121 gcgcaagcac gaacgaacgt tccgcaaact tgcgaagtag aagcccatga gtgctcccga
     3181 gttcttgacg gtggaggagg tcgaagctct ccacagcgag caactccgcc tctttggtgg
     3241 ccttgagggt ttgcgcgacc gcggcgctct cgagtcggct gttgccgtac cgacagcgac
     3301 gttcggaggc gagtttcttc acgttgggct cttcgcgatg gcggccgcgt acgcttttca
     3361 catcgccgag aaccagccat tcgtcgatgg gaacaagcgc actgcactca acgcggcgct
     3421 cgtcttcctc ggcatcaacg gatgggaagt ggacgatccc gatggcgctc tctacgacgc
     3481 catgatcggc atttctgagg gtaccatgtc caaa
//
LOCUS       DASTKU010000615         4704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1681761, whole genome shotgun sequence.
ACCESSION   DASTKU010000615 DASTKU010000000
VERSION     DASTKU010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681761"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFQ61_21545"
     CDS             <1..189
                     /locus_tag="VFQ61_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957102.1"
                     /translation="CSGPGLDMNRDARTAGKWATAAFAVGATGLVAATVLWITAAPRT
                     NNVQVGLAGGSLRVRGTW"
     gene            186..1778
                     /locus_tag="VFQ61_21550"
     CDS             186..1778
                     /locus_tag="VFQ61_21550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957103.1"
                     /translation="MNRRNAGWGWVFACLPAMMACNAVLDIDQKKPGELPPTSTTGSF
                     ELTASSSVRLVAGGQAALEVTVQRRDGFGGPVTALVNGLPHGVRAEPLVLAASQTSGK
                     VLFRASESAEFGRSSISILGASQSLILDPISVELVVQGTPGSLDETFGDHGRVVLAPT
                     GATELGVGAGGLRIAQDGSVLLCGRVRTASSERSVLLARLTSEGELDETFGSEGWVFT
                     NSQRSQSDECNSAILRPSGGVVFAGAASPNGSPGDLMAARFSSTGHASGDFGKSGFIT
                     RAFGEKQSSACCVLGGDQDKIIAGGQVDGDALLIRFMKNGEIDASFGSRSGSVIDGAG
                     MRSLAQQSTGHFVAALDSDTFRVARFNPDGQLDESANPQPVGVDVGGRGSSKAVIALV
                     RPDDEIVVVGTAQAETGFAELAVAFLTNTGQLDTSKLSEGWVTSHWGEGDVTVSGAVL
                     QADGGLLVAGETLSADGALLTLTRLEADGSLDTSFGTGGRRTFEMERSAHSIALDALG
                     RVLVVGSAGATDSALVLYRLWP"
     gene            complement(1824..2249)
                     /locus_tag="VFQ61_21555"
     CDS             complement(1824..2249)
                     /locus_tag="VFQ61_21555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957104.1"
                     /translation="MHPKTTLSIHCIGVLLALCWFPGCGRSCFSRGADLYSRGDYVDA
                     EEYFEHTEARLGEADPQERARYALYRASTLLALGDVDRAEHWLARAKHLDRIDPGALD
                     EDEQAMLRRAQATAAVASRRRERTQRAYTQINAVQLASP"
     gene            2537..2935
                     /locus_tag="VFQ61_21560"
     CDS             2537..2935
                     /locus_tag="VFQ61_21560"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HET9957105.1"
                     /translation="MTSIEIELTTPALLFPAISLLFLAYTNRFLVLAQLIRQLHGSNR
                     EQVKEVVVRQIENLRERVRLMKIMQLLGVVAFILCSLSMTALFFSQRTAGRVLFGGSL
                     FCLVSSLLVSLYEVTLSTGAIELELSDLER"
     gene            3156..>4704
                     /locus_tag="VFQ61_21565"
     CDS             3156..>4704
                     /locus_tag="VFQ61_21565"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957106.1"
                     /translation="MPPFSSRTLRQSGPALLAIWFVTHCSSESQSVHRDVAPAEGEGG
                     SPAAGASGMAGDGIAGAQAEGARAGRDSSSAGGDNNVAGSAEPPDTQNSGGTGARGEG
                     GQPTGGTDSAGESSGAGGAGGSEGTESGCSIAGVLVPADERNPRAACEVCDPHRSTTS
                     WSRLGFCPTSIAAGGRRTCGLMDGAVYCWGDNQDGALGDGTTSDALQPIRVESLDGET
                     SALMVGGRHTCTITGSAAYCWGYNGYGQLGDNSTSDQSSPIRVLDDLPASSQIVAGGS
                     HTCLLADGAVQCWGNNESGQLGIRSTVNQRLPTPLQALAAGVEAISAGVLHTCAIQEH
                     AALCWGANDKGLLGNDSEDKSTVPVPVQGLSSGVTAISAGDGHTCAIVNGAAWCWGYN
                     EFGQLGNGSSKNSLVPVRVSGLTSGVTAIAAGGLHTCAVVKGAALCWGSNGYGQLGTE
                     TGETIMVPTPVAGLNQGVTAVAVGAVHACAIADGLTYCWGYNASGTLGDGSQTTSHVP
                     VLVTPPVE"
BASE COUNT          822 a         1358 c         1622 g          902 t
ORIGIN      
        1 tgttcggggc cgggcctgga tatgaatcga gatgcgcgca ccgcgggcaa atgggcgacg
       61 gccgcgttcg cagtgggggc gaccggttta gtggctgcga cggtgctttg gatcacggcc
      121 gctccgcgca cgaacaatgt ccaagtaggg ctcgccggtg gctctctacg agtgcgaggc
      181 acgtggtgaa tcgccgcaac gctggctggg gttgggtgtt tgcgtgcttg cctgcaatga
      241 tggcttgcaa tgccgtattg gacatcgatc aaaagaagcc cggcgaattg ccgcctacct
      301 cgactaccgg cagtttcgaa ctcacggcgt cgtcgtcggt acgactcgtc gcgggaggtc
      361 aggcggcgct cgaggttacg gtgcagcggc gagatggttt tggcgggccg gtgaccgcgc
      421 tcgtaaatgg gctaccgcac ggcgttcgcg cggagccgct cgttctggcc gcctcgcaga
      481 cgagcggcaa ggttctgttt cgtgcttcgg aatccgccga gttcgggcgc tcgtcgatct
      541 cgatactcgg tgcgtcccaa tcgttgatcc tagaccccat ctcggtcgaa ctcgtggtgc
      601 aagggacacc gggcagtctc gacgagacgt tcggagatca tggccgcgtc gtgttggcgc
      661 ccaccggggc cacggagctg ggcgtaggcg ctggcgggct acggattgcg caagacggct
      721 cggtgctcct ctgtggtcga gtgcggactg cgagttcgga gcgtagtgta ttgctggcac
      781 gcctcacgtc agagggcgag ctcgatgaaa cgtttggcag cgagggctgg gtgttcacga
      841 acagccagcg ctctcagtcg gatgagtgca actcagccat cttacggccg agcggcggtg
      901 tcgtgttcgc gggcgccgcg agcccgaacg gaagtcccgg agatctgatg gcggcgcgct
      961 tcagctccac cggtcacgct agtggtgact ttggaaagtc agggttcatc accagagcgt
     1021 tcggtgagaa gcaatcgagt gcatgctgcg tgctcggagg cgaccaagac aaaatcatcg
     1081 ccggcgggca ggtcgacggg gacgcgttgc tgattcgctt catgaagaac ggcgagatcg
     1141 atgccagctt cggctcgcgt tcggggtctg tcatcgatgg cgcgggaatg cgttccctgg
     1201 cgcagcaatc gacgggtcac ttcgtcgccg ccctggactc cgatactttt cgcgtcgcac
     1261 gcttcaaccc ggacgggcag ctcgacgagt ctgccaaccc gcaacccgtg ggagtggacg
     1321 tgggtgggcg tggctcgagt aaagctgtga ttgccctcgt ccgcccggac gacgaaatcg
     1381 tggtggtggg caccgcccag gccgaaacgg gcttcgcgga gctggcggtg gcattcttga
     1441 cgaacaccgg tcagctcgac acgagcaaac tgagtgaggg ttgggttacg tctcactggg
     1501 gcgagggtga tgtcacggtg tccggcgcag tgcttcaggc cgatggcggg ctgctcgtcg
     1561 caggggaaac tctgagcgcg gatggtgcac tcttgacgtt gacacgcctc gaagccgacg
     1621 gatcgctcga cacctcgttc ggtacgggtg ggcggcgaac gttcgaaatg gagcgttcgg
     1681 ctcacagtat cgcgctcgac gcgttgggac gcgttctcgt cgtaggatcc gcgggcgcga
     1741 cggatagcgc gctggtgttg taccgtctct ggccgtgaac atcggtgggt ctctcgagcg
     1801 gaaaccccgc ggagtgaccc gcctcaaggc gaggcgagct gtacggcgtt gatttgcgta
     1861 taggcgcgct gcgttcgctc ccgtcgtcgt gacgcgactg cggccgtggc ttgcgctcgc
     1921 cggagcatgg cctgctcgtc ttcgtccagt gcgcccggat cgatacggtc gagatgcttc
     1981 gctcgcgcta accagtgctc ggcccgatcc acgtccccga gcgccaacaa tgtcgacgcg
     2041 cgatacaatg cgtaccgcgc tcgttcctgc gggtccgctt ctcccaaacg agcctcggtg
     2101 tgctcgaagt actcttccgc atcgacgtaa tccccacggg agtacagatc tgcgccccgc
     2161 gagaagcagg aacgaccaca ccccggaaac cagcacaagg cgagcagtac gccgatgcag
     2221 tgaatcgaga gagtggtttt gggatgcatg atgagaatga ggtagcaagg gcaatgccga
     2281 gctcgagtct ccgccgaggg ctctaaagtt gccccagccg ggcgcttttg ctgccccaga
     2341 tacagcggag agacctagac agagactcgc gcccggaacc gcggcgcgcc attcacgcgc
     2401 ggacgtcagt caggcacgcc cctgcccccc cgaggttcag ggcgtccaat cgacctcatg
     2461 acgcactccg ttgagccaga ctcccgagct gtggcatcgt tccgtgccca cgaggcgtgg
     2521 gccccaattt acgcgcatga cgtccattga aatcgagcta accacaccgg ccttgctctt
     2581 tcccgcgatc tccctgctct tcctggcgta caccaatcgc tttttggtac tcgcgcagct
     2641 gattcgccag ctgcacggaa gcaatcgcga gcaggtcaag gaggtggtcg ttcgccagat
     2701 cgagaacttg cgcgagcgcg tgcgtctgat gaagatcatg cagcttctcg gggtggtggc
     2761 cttcatcctc tgcagcttgt cgatgacggc gctgttcttc tcacagcgca ccgcgggccg
     2821 agtgttgttt ggtggcagct tgttttgctt ggtgagctca ttgctcgttt ccttgtacga
     2881 agtcaccctt tctacgggtg ccatcgaact cgagctctcg gatttggaac ggtagctagt
     2941 tcagcgcatg tgagatggag acaacgattt ccggcgctgc actaggcggg cgtgatctag
     3001 attcgcggac tccctgtcgg ttctcgccgg tatgcggcgg atgagtacgg tgtccggacc
     3061 ggcgcgggtg gacggcaggg catacgatcc gcggaaaacg catagccagg ataatcgatg
     3121 ttatgctctc atcgtggctt tcgaggaggg ctcgtatgcc accgttctcg agtcgcacgc
     3181 ttcgtcagag tgggccggct ctgctggcaa tctggttcgt gacccattgc agcagcgaat
     3241 ctcaaagcgt tcatcgtgac gttgcgcccg cggaaggaga aggcggttct cctgcggcgg
     3301 gcgcgtcggg tatggccgga gacggtatcg cgggcgcgca ggccgaaggc gcacgagccg
     3361 ggcgagactc ttcatccgcc ggcggggaca acaacgtggc tgggagcgcc gagccgccgg
     3421 acacccaaaa ctcgggtggc acgggcgcgc gcggtgaagg cggacagcca acgggcggca
     3481 cggactccgc gggagaatcg tcgggcgccg ggggcgcggg tggatcggaa ggaacagagt
     3541 cggggtgctc tatcgcgggc gttctcgtgc ccgccgacga acgtaaccca cgagccgcgt
     3601 gtgaagtctg cgatccacac cgatcgacca cgagctggtc acggctcggc ttctgtccca
     3661 catcgattgc tgcgggcggg cggcgcactt gtggcttgat ggatggtgca gtgtattgtt
     3721 ggggggacaa tcaggacggt gcgctcgggg acggaaccac cagcgacgcg ctccaaccga
     3781 ttcgagtcga gtccttggac ggcgagacca gcgcgttgat ggttggcggg cgtcatacgt
     3841 gtaccatcac cgggagcgcc gcctactgtt ggggctacaa cggatatggg caactggggg
     3901 acaactcgac ctcggaccag tcatcaccca ttagagtttt ggatgacttg ccggcttcca
     3961 gccagattgt ggcgggagga agccatacgt gtctgcttgc cgacggagcc gtgcaatgtt
     4021 ggggaaataa cgaatctggg cagctcggca ttcgctcaac ggtcaatcaa cgtctgccca
     4081 cgccactgca ggccctggcg gcgggggtgg aggcgatctc ggcgggcgtg ttgcacactt
     4141 gcgcgattca ggagcacgca gcgctgtgtt ggggcgcgaa tgacaaaggc ctattgggta
     4201 acgactcgga ggacaagagt accgttcccg tgccagtaca gggcctttcg agtggggtca
     4261 cggccatcag cgcgggggac ggccacacct gcgcgatcgt gaacggggcc gcctggtgtt
     4321 ggggttacaa cgaattcgga caactcggaa atgggtccag caagaacagt ctagtgcccg
     4381 tgcgcgtgag cggactgacg agcggagtga ccgcgattgc ggcgggaggc ctgcatacgt
     4441 gcgccgtggt caaaggggct gcactctgtt ggggctccaa cggatatggc cagctcggca
     4501 cggagacggg ggagacgatc atggttccta cgcccgtggc gggcttgaat cagggcgtga
     4561 ctgcggtagc agtaggcgcc gtgcatgctt gcgcgattgc agacggtctg acctattgct
     4621 ggggttataa cgcctcaggc acgttgggtg acggttcaca gaccacgagc catgttcccg
     4681 tcttggtgac gcctccggtc gagg
//
LOCUS       DASTKU010000616        30053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_565706, whole genome shotgun sequence.
ACCESSION   DASTKU010000616 DASTKU010000000
VERSION     DASTKU010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 30053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30053
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565706"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..2723)
                     /locus_tag="VFQ61_21570"
     CDS             complement(333..2723)
                     /locus_tag="VFQ61_21570"
                     /inference="COORDINATES: protein motif:HMM:NF027452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HET9957107.1"
                     /translation="MAVAPGASTTVLDAASTLAHYLGQITGRHLDVRVPQGVDAKLPQ
                     IKLETNSDGRGDVSSWVTSAPVAAETYVIRSEPERLLILAATDHEILSAVWDFLHQVG
                     YRRYFPGATWQVIPHASQLEIQTERTCRAAFRQRRLFLGAGAYRENKAEFAEWSRENR
                     LGGLEIVTNHSYQKIVAANPAAFAAHPEYLGDGRAGKSPKFCVTAPGLVELVKGYAAQ
                     YFAQHPDSISISMEPSDQGGWEGCPGDTKLGSPTNRAVTLANAVARSLQAGSGAPHFV
                     GMLAYYEHSSPPTLPIEPNVAVFVATAFSRSGLDFMETFRAWQAAGLRPGGTWMGVHD
                     YYSVNAWDRDLPGQAASSSPSQIVASLETYHSAGAQLFDAEIGDGWAPNGLGYWIAAA
                     TLWNADRSPVPSSYVDDFLSHAFGRARHVMSRFYESIDQSAHPLLCSEFVGELYRLLE
                     RARVLEPKPAVAARLDVLAEYLHYLDLWIDYAFEKGSGRQSAFEKLMVYSYRIRRTHM
                     VDSFALRRDASIRDREIVAPDPALWRDTAAEPWRDETLVTQAEIQSWIRAATRRAPLP
                     PARQLDRVDSLVRLSPHDLHNDRGLHGITLRGQQRFNLLLDPAVPLHLVVRGGDVRAI
                     GEVRIALCPSGATIEECQHTSVPADRADHEVSFQLPGPGHYELDVDDTNSGVDLSWAA
                     GTPIVIAATARALPALRHSWTMYVYVPAGTRELVLYSSGGTGVLRGPDGTIAHRFTRR
                     PGVIRIPVPRGHDALIWKFENNSGYRLPLNIPAWLAASPEELLVPARVLPELVPHG"
     gene            complement(3088..5694)
                     /locus_tag="VFQ61_21575"
     CDS             complement(3088..5694)
                     /locus_tag="VFQ61_21575"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET9957108.1"
                     /translation="MSKLTLQLSSAYTLALGLGLSALGAGAVHLEFLAPFALLVIASA
                     SCVLLQKERDDTQHYFDRRAFVCIAVWGALALICLLQWVRLPLPWLQKIAADNADIWA
                     RALRPFGLPEPKLASISLAPGRSVLEALKVACYGMIFAVSARLSRDWGTRRLLMIAFA
                     VPLLVAVVTAAHQLVGARRLFGIYEPVDAYTIAPLLNANSLGGYLNLGLFCGLGLAFR
                     AGARPTAALFGVGIAFIAAEVLLCESRGASGALAIGLFLVLALPRGVRNPKRAPELSP
                     LLQATVVVSIGVAGGLLAWAARHSPLGLDDRSLSKVELFSRSWQLAKDHFLLGVGRGA
                     FASISSTYQTGGPYIISEHAENLPLQWSAEWGVPVAIGALAALLWSLRPIVCRSTWSV
                     PTRRCALIGCAVVLIQNVVDLGLELPAYAGLLVFVLGALSGSTPSSLPTPRWLSARRV
                     LAFGTALTALCLGLSLRYGVVAPSRLRHELHDELVAAHGAPTQEFWDALGRAVLAYPA
                     EPYFPLLGSSASLMVGRDPLPWITRALERAPQSAQVHLELAKVLKSGGAIQQSLGALQ
                     RCVELDPRQTPGVLNLASGWGVSPEALTAVAPNGQAGAAVLAQLALRATDRALRVQLL
                     EQSLARHSEQPDVHYQLATELLDDLGRGEGGTVCGSQRDVCLTQVAEHLQHGAVPDSP
                     RSLIAAARLLAEQGNPLDAENKLASGCARFPGDLKCATALFDLALDNGSPRLLDATRE
                     FVAASCVNLERCGAAHRRLGDKFASSGRWNMALTHYQRAARDAPSAGAWRAVALAAEH
                     VGEPELAADARRRVTILDSTERMAPAKPVWSGSSVPAMPDMDDMGAQRSSSRLGPARV
                     EEATQREPSNER"
     gene            5876..8116
                     /locus_tag="VFQ61_21580"
     CDS             5876..8116
                     /locus_tag="VFQ61_21580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HET9957109.1"
                     /translation="MTAIPNLSANPRAAVPVRKDTSKTLLDAWKVLRKRWAWMVTLAL
                     ATIGAAGFYTAGLQRIYLSSCVIEIDPKPPKPLGQEVQAIVDVGNSSYWANSEYNKTQ
                     FQIIQGRAVAEETVRRLGLHRNPAFLQGLSPNQKPADGAAQETSVAAAAQMVRGHLVV
                     EPIRDSRLVTVSYRDPDPQRAHQILSTLVGVYVDRNIDVALDSTSSAAEWLRGQVDNL
                     KQELDGSELALHEYKVSNRILSASLDDQSNMLRQEMQQLSSALTAVRVRRTQVAAQAR
                     ELTDFERQGSEWLPKDDVLASETLQRLRETLSQAVAERDSLIGSGKGEMHPLVVAASA
                     RVNAAQDALQHEIKNVREAVQRDLRVIDQEIASLSELFEQAQGRALSLGKLEIEYRRL
                     DRNKLNTEKLYSMVLERSKESDLTLMMRFNNIQVIEPPQVPELPVSPRVPLNMGIGVL
                     CGLALGLMGAFGREMFDQSVKTAADVEDLGITLLGVVPLLGESRSATYGRRQKARRRQ
                     QQDARTELIVHADSTNAVAEAARGVRTNLAFMSPDKPFRSLLVASGGPSEGKTTVACW
                     LAIVMAQAGHKVVLVDCDLRRPRLHRVFNFTNDRGVTNALVERSTLADAIRPTEVPGL
                     SVLLSGPRCPNPAETLQSKSFETLLADLLATYDRVVIDSPPVGPVTDAVILSTRVDAT
                     LLVVRALFSARDMVRQAKRLLQDVRSNLAGAVLNAADQHRQGYPYYRYYGRHDADEAP
                     SQEDAT"
     gene            complement(8109..9257)
                     /locus_tag="VFQ61_21585"
     CDS             complement(8109..9257)
                     /locus_tag="VFQ61_21585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9957110.1"
                     /translation="MRVAYLSGSEIPSRAANTVHVMKMCAAFAGQGHQVDLWARTTRH
                     LASSAFRDFGVPPVFRIHAARWPPLPFGIGSGTFALQVMAGAHCSRPDLIYGRHLPSI
                     FGLASMGSLAALEMHTPPENALKATLIQRLLRMPAFLGIVVISDALKQHFLGLFPTAE
                     RHIFVAHDGADPPCEERTEFGRKAQNNGRPQVGYVGHLYPGRGIELILELAQRMPAVD
                     FHLVGGTDADLRRWSQSAGEQANIRFHGYVRHADLARHYASFDVLLAPYQSRVAISGG
                     AGDTSRWMSPLKLFEYMAQRKPLVVSDLPVLREIVQHERTALVCRADDVRDWQSAIER
                     LSQDEELSRRLSAAAYYDFNRFYTWTTRAQAILNFLDAQRCAKRIQIT"
     gene            complement(9427..10146)
                     /locus_tag="VFQ61_21590"
     CDS             complement(9427..10146)
                     /locus_tag="VFQ61_21590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9957111.1"
                     /translation="MAHDNFCIRTQYIARSTALTHEGTSGETYWTAARIRAADTYQWG
                     VYALAERELRRRAARTVVDVGCGPAPKLAALQKRNPDVSFIGIDQGSAVTYCRGHYQW
                     GDWLEEDLANPKQTPSDIQGDVVICADVIEHVLDPDVLLDYLKQHVTPCGLLIISTPD
                     RDLLRGPNCTQSPNPQHVREWNGEEFKRYLQSRGLRVTRQIRQLPIRLRLGRPLLDEL
                     ILRAVRGQPLRYNQVCLAEVS"
     gene            complement(10220..11197)
                     /locus_tag="VFQ61_21595"
     CDS             complement(10220..11197)
                     /locus_tag="VFQ61_21595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957112.1"
                     /translation="MNLGGTPTAILRMYDRFGTLALQNLAASVTRLLACATALFFGCS
                     VLGFGIASLCGDIASNLFLLVASCLRLRAEGVARIWSANARGHIQRNRSLVQFALSSH
                     LNSTVRVSRELDVMVVAAFSSASAAGVYKAGHKFASFLSVLTDPFYQVLYPELNRIIV
                     AGDLSAFRRTMRFGAVAAGLSAAGALLIGLLVAKPMIAHLLNVQFTDAFGVFAICGIG
                     WVLWGFSTPLTPALLAMGKAPTALAVHAAAAATYLPLLIWLASIVGAIGAATAFVAFN
                     TIWAALMLLVLQAEFSLWSKSRQEASVQLSSATAAAQPGSRGNPLGDSS"
     gene            complement(11618..13366)
                     /gene="asnB"
                     /locus_tag="VFQ61_21600"
     CDS             complement(11618..13366)
                     /gene="asnB"
                     /locus_tag="VFQ61_21600"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET9957113.1"
                     /translation="MCGIFGHTLVTQSYSKEHSRDSLDTLRHRGPDSAGEWTDERVYI
                     GHRRLSILDLSERGRQPMVSPDGSVVVAVNGEVYNYRELRDELGRNRFRSNSDSEVLV
                     HGYQDWGIEGLCARIDGMYAFVIYDAREHMLYLVRDRVGIKPLYYGWCDGVFAWASEL
                     KAIQRHFNGKLELDVTALYDFFTYLYVPSPKSLYKNIQKLEPAELLQLDLKNKTTRRS
                     RYWHPPEPGSLRLTRAEAQQELQHRLARAVQAQLVSDVPVGFFLSAGVDSASVVAAAV
                     DAGATPRTFCIGFDDDKRSEMAGASATAKFLGAWHRNECLNADGARELHATVRRLYDE
                     PFASGSALPTYLVSRAARRECTVALTGDGGDEIFGGYLRYNLYERVLLARRFVPTRFS
                     GYLKALTSRIPRELDPRLRSLARRLESAFVHEPLAVHTRLIGGLVHEEKAAYRERWSI
                     PEDYDDYWSFRRYYRKDLPAVTQQQFLDFHTYLPDDILTKVDRASMAVALEVRVPLLD
                     IQLVELMFALPESIRLCGRELKGLFKAALSERLPRAVLRRGKTGFSVPMATWGKAYFG
                     EQSSIQDRTLELLGLS"
     gene            complement(13600..14823)
                     /locus_tag="VFQ61_21605"
     CDS             complement(13600..14823)
                     /locus_tag="VFQ61_21605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9957114.1"
                     /translation="MVRLPMLKVLHVITGLNQGGAERALMRLMLHEDKSRFSSEAFSL
                     IGDGLVARQMREQGLRTLNCGPRRGGLSATLMSLIAHCLRSRPDVVQGWMYHGNLAAT
                     LAATAACVPLHGWNVRCSPSERLEEKLGTRLAVSLGARLSHLPTWIIHNSYRGEAQHR
                     ARGYSPTRATVIPNGFDCEALRPDDALRTSLRNRHDVSAQALVVGMMARHHAQKDYPG
                     FIRAAARIAAQFPTVCFIAMGRGVDWSNDALRMQIEELGLRHRFRLLGDVPDGGRCAT
                     MFDVNVLSSAFGEGFPNAIAEGMACGVPCVATDVGDTAWIIGDTGRVVPSSNPVALGS
                     AVSDLLGRTREEREKLGALARARVLREFSMTKNVSNYAALFDAGYSSRGRTVEMADTI
                     SNSPQQPATQTGSRG"
     gene            14966..16600
                     /locus_tag="VFQ61_21610"
     CDS             14966..16600
                     /locus_tag="VFQ61_21610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957115.1"
                     /translation="MTENKCRLLRATWIEQMNGEVIAILATLTLGAALRHAMAAVPPL
                     PEVAYRNSVIFLQALRHPGYVLALLAGSSVYILTQRGRQIVRWSMLEPVHLRWFVVLI
                     TWPLAWYYSTYDYNFYFDQTHGWDRALLVLLWLGVLASPALLLPFLLQCVILIRQFDY
                     PDVLQYSWTDKSLPLAFACCFWAYCIVACFTRRAELKRLLIVVMAIFAAHYFGPGVQK
                     VSLGEHPLDWALNNSLHHLFVAATRSGWLDRVDPMSVMSIAHWLERATIPLQLATLAF
                     ELGAVLVFWDRRAVLAAALGAVFLHASIFLSSGILFWKWVIVDLSVLAFVGVIWREGM
                     SHIAAERKLAAIATLLVPLATVIYGVPLLGWHDTQYLASYEIMAQDTSSRHFSLSRQF
                     FAPYDMVFSQNRFRFLAPQPVLPVGTLGSTQDSDLRREIAAATPETLPALIERLGVRR
                     FDPDKKRSFNAFLADFIDGANRRGIHRVVPEWLRAPSHIWLEGGENPQPLATPITHAS
                     VEFREYLLDPFPSHPYRRAVLDDVHIPISLRGGFSR"
     gene            16689..17822
                     /locus_tag="VFQ61_21615"
     CDS             16689..17822
                     /locus_tag="VFQ61_21615"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9957116.1"
                     /translation="MRRILIVAGLAESLVRFRGDLIQSLLARAIEVHVVAPCGVQPRS
                     WETLRSWGVVLHQMSLDRTGLNPAGDMRVIAELYRLLRRTRPTYLLAYTAKPVVYGLL
                     AARLAGIQRTTALITGLGYAFSPESGIRRRVIRHLLESMYGFALGGAHRVVFQNPDDM
                     RLFLDLGLAKPEQCGLVNGSGVNLEKFPDTALPPLGSSMRFVLVARLIRDKGIYEFAE
                     AARSVKHMCPETEFHLVGGHDSNPAAISRAALEQWIAEGSITYHGQLEDVRPVLAQSH
                     VFVLPSYYREGIPRTVLEAMAMGRPIITTDAPGCRETVVQGQNGYLVPVRDSGALASA
                     MLHFVERPELIQIMGTASREMAVRKYDVAKVNSAMLAHIGVDG"
     gene            17875..19038
                     /locus_tag="VFQ61_21620"
     CDS             17875..19038
                     /locus_tag="VFQ61_21620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957117.1"
                     /translation="MTSVGVLFVVLGAGLGLWFSVKWRSPVYLALFVFHLMASLFFWN
                     YSLHHPADAVEYYRYVNYYSEIAIGTTLVAWLTATIRDALVASYLDVFMAFHLFGYLA
                     LVILFRLCAAVMSDFQEPDAVDGQQSLGRRALLYSMTFLPGLHFWTSPICKDGLALLG
                     ITSFTWGMVRPERRLLALVFGLFAVGMVRPHIAALLAAAGALALVFSRDSSAGLRLLL
                     SVSLLAGIAVSLPFLQSYTGLDELTAQGVGAYVDQRQESNLVGGSVVDISQYSFPMQV
                     FTYLYRPMFLDASGALGLIVSFENLYLFALSLCLPFQLRRLWRSFEHQAFLRFNVIYF
                     CAVTAILASTTANMGLALRQKMMGLPSLLVMVLMSLSAREPVEELLADEAVSD"
     gene            19090..19830
                     /locus_tag="VFQ61_21625"
     CDS             19090..19830
                     /locus_tag="VFQ61_21625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9957118.1"
                     /translation="MTEYFENPSLYFRGRYVVDLRKTLARDLLGELKETRILDLGCGD
                     GTVSLQFLGNGNQLTLADSSRAMLSLVRASVPKHLENTVTLVESALEDLPDQQFDIVI
                     CLGVLAHVQSVERAVQKIAASMRPGARCLIQITDHDRRIASLVRLFAGVRHLLQQCGR
                     YEVNVTTRAQLEQCAGNLGLECLESKAYSLGIPGAGRLLSQRVLYKYAELSLRSTMMQ
                     PLCSEVLLLFKKQVGHAVLATAGRGNDY"
     gene            19848..20513
                     /locus_tag="VFQ61_21630"
     CDS             19848..20513
                     /locus_tag="VFQ61_21630"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET9957119.1"
                     /translation="MERSHLEKLAKRTLDVGVAGIGLVMSLPLLVGVGIAVRRELGSP
                     VLFRQIRPGLKERPFEMIKFRTMCEPTLGQDRLDSDAERMTPLGKFLRASSLDELPTL
                     WNVLKGDMSLVGPRPLLPEYLELYSREQRRRHLVRPGVTGWAVVNGRNALDWDSKFAL
                     DVWYVDHWSLGLDLKILAKTVVQILLRTNVVHEGHATMPRFRGSVINEADDSTTLQER
                     REP"
     gene            20942..22240
                     /locus_tag="VFQ61_21635"
     CDS             20942..22240
                     /locus_tag="VFQ61_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET9957120.1"
                     /translation="MGLLEQFSAKQGHIVVVGVGYVGLPLVAEFVREGFQVTGLDYDA
                     RKVELLSQGESYIRDVPSSDLAAHAKSGRLRATADPAVLREADAVIVCVPTPLNKTKD
                     PDMRFIVDAAEQIQKHQHPGMLVVLESTTYPGTTTELLVPKLTERGFELGKDVFVAFS
                     PERVDPGNETFKTRNTPKVLGGATPQCLAVASALYSHVIAKLVPVSSTETAEMVKLLE
                     NTFRAVNIGLANEIALMSRKLGVDPFEVIRAAASKPFGFMPFYPGPGLGGHCIPIDPL
                     YLSWKLKSLKGQARFIELADSINGSMPNYVVERVAAALNEHSKSVKGAKILVYGIAYK
                     KNVNDVRESPAFEVIEELEKKGAHVAFTDPLVPTVELPNGESQAGVAADFAAYDAVVV
                     ITDHDALDRERLAREGRIIIDTRNALAGIKDLDRKRVFGL"
     gene            complement(22267..22899)
                     /locus_tag="VFQ61_21640"
     CDS             complement(22267..22899)
                     /locus_tag="VFQ61_21640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET9957121.1"
                     /translation="MTEGSAVVVYGAGGHGSVVADAARCAGLDVLGFLDDQRPVGDSL
                     ALGRVLGRSGWWRDHPGVSIVLAVGDNFARQRICADLESDGRKPLTVVHPRAVVSATA
                     SLAPGVVVLALATINPCACIGIGAIINTGAVVEHDVHVGAFAHVSPNATLAGAARLGE
                     LSHVGCCACVLPGVRVGERCLVGAGAVVTRDVPHNSVCMGVPARVTRQLG"
     gene            complement(22900..23910)
                     /locus_tag="VFQ61_21645"
     CDS             complement(22900..23910)
                     /locus_tag="VFQ61_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957122.1"
                     /translation="MRRLQAISAMAALTTLGASACEALIGDVEVTRRPRQAMDTLQPN
                     PAPVQGGLSFPPFPNGALPSTPAPGVGRPESDAGVTAPGITDAGGMAPDAAPPAPSAR
                     PILVDAPTQLELVGKVGGGPRLGTCQGGVVVGVRATANPSTETFGQRLAFIEPLCASA
                     TVGGAAITIRRDDALVNWSMSEPFLGVPSLEVPDTRLTWVPQPPAFCPDSAPVLVGLS
                     GQYDPIAPDDTETSALRSLVIECAPLTLAANGVDLAISSSGHLLISQADNFSASGSEA
                     YRSSCPEGAVVTQMQLHSGFWLDGFVLGCSALRAPRAKGEVCGEGRECSSGVCMGGIC
                     GG"
     gene            complement(24144..24806)
                     /locus_tag="VFQ61_21650"
     CDS             complement(24144..24806)
                     /locus_tag="VFQ61_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957123.1"
                     /translation="MSRTLQARPTLLLCAALSSAVLAACAGADPPVVSSRLAGQIRAQ
                     YELDGGVPSNGGSGGGAASAPSNAVASAAGGASAAPPPATPADNTGTGGSGGAANQPE
                     DTGDAEAGSGGSAGSPASSSCDGFAVLAANCGSSGCHGDGSNLENFAASETAARAFID
                     KPGTLACTGQGNVIDPDDPDGSLMIQKLSADPPCGSTMPLGGVTLSKSDIDCIRSWMG
                     NL"
     gene            complement(24984..26456)
                     /locus_tag="VFQ61_21655"
     CDS             complement(24984..26456)
                     /locus_tag="VFQ61_21655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957124.1"
                     /translation="MGHKPGEILGGRYRLIRPLATGGMGSVWKAEHLSLNAAVALKLI
                     EGPAQLDSSATLRFLREARLAAALRSPHVVQILDQGIDGTTPYIAMELLEGESLADRL
                     ERVGRLSAAETARVIQHLARAMTRAHEAGIIHRDLKPENIFIVRNDDEEIFKVFDFGI
                     AKVEAPVGNATRTGSLLGTPSYMSPEQAEGAKTLDQRTDIWALGVIAYRCLLGQLPFC
                     EPTLPQLVLAICTRPLPVPSRKGSVPEGFDAWFARACARSPADRFASARRASAELSAV
                     LGAPSALEEMPSSATNPLLSSVDPPAAGALKPPPRYLAAPALPASAVTPRPVAASLPI
                     ANARSEHGARRSKSAKHWALPISLAIALPGAALGVSRVTASLEQRALASAAAPALEQV
                     TLSLAAPGAAARDGAGLPDLPAGVRLANNVRVVPSLRPAAAEPAAPVPPPAPLPTPAV
                     TVQPVDGTSSARPGRGAQALDAPEPRRPAKRRARQGAHSR"
     gene            complement(26721..27467)
                     /locus_tag="VFQ61_21660"
     CDS             complement(26721..27467)
                     /locus_tag="VFQ61_21660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957125.1"
                     /translation="MPGKTVTGWLAGLALAPLTGSVSAVRRSRMFHPRGVLYRAEVEA
                     APASSALAGLASALAGPALVRWSSAWWKRGERTDVLGCAIRFGSDPLHAAPQPGDQDL
                     LLATIRRPWSMAFAPWTTDFHDFLANDYYGVSPFQVGTRRVEWRLAPEREAPPGPTRS
                     ERLERAVRAGEASLRLELAAYSGPLFPPVGESFRSVVQLHLREPVELDQEALRFDPFR
                     DGRGITPVGFVHSMRKLTYLASQRARPRHR"
     gene            28105..29319
                     /locus_tag="VFQ61_21665"
     CDS             28105..29319
                     /locus_tag="VFQ61_21665"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957126.1"
                     /translation="MERILSTGSLGAVVSARHLVRDEIVVIKLLHPDLCHDPVSVARM
                     SREAHVLAHIASEGVVRLLDTGWTDEVGPYVVLEHLVGMDLARLLETSGPLPLALAVD
                     YLLQACEALAVAHASGITHRDLKPENLFAVRGFCGDTPHPEGAAPLLKLLDFGIAQHG
                     PESFPYREIALEDAEAPLLGTPAYMSPERIQDMPTDQRSDIWSLGVVLHELITGRVPF
                     SRHDRSNLQRGEESDICDRIVSHSFELENNRSLLPSPLRAVIHRCLQRNPAHRYQSVE
                     ELAAALRRTIALPERWRGTQTGKFSRQLLAQELAQAEAAAPPAAPSRWSSAAQEIGAA
                     LQRLPARLAPLAAALRGRAPRPHALRGWVRAHWGFGAWLATALTLAALAGGLLAKLEQ
                     SRAAVAPSALSQ"
     gene            complement(29411..>30053)
                     /locus_tag="VFQ61_21670"
     CDS             complement(29411..>30053)
                     /locus_tag="VFQ61_21670"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9957127.1"
                     /translation="ALDLPSAAQLPAARARLRNTMHYTDDVVRELYESLRDEPWFGRT
                     VWIITGDHAFDLGERGEVLGHDNLRHETTWVPLIVHGSDERLPRGAQRRIASHVDLAP
                     TISELAGIHDDNGYMGHSLLDPQSPNASALILRGWHYACETPQFSLFRPAQGTPIAYA
                     PDDLAQVREPIALTASELGRAERWARATQTLLAYVVDFDRQTPKEDARWLSRK"
BASE COUNT         5604 a         9199 c         9605 g         5645 t
ORIGIN      
        1 tgtttgcgga gaggctcgac gcgggggtcg agcaggcgag tcttcttgcg tcccactggg
       61 ggctcctgtc gtggcgggtg gacccgctca cgctacccga cgcgcggcaa cggctcatgc
      121 acgcttgccg gaagcacacg cccgactgat tgccttcgcg tcacccacgg tagagtgagg
      181 cgaagcccgc tccgacgcta cacggctccg tgaagcggat acggcttccc ttctcacccc
      241 agctcacgag tccccagagc gagaagatta ccccggccga tcacggccga gcgcgagagc
      301 caacactgac tctgcgtcgg gttcgacggt aatcagccgt gtggaaccaa ttcgggaaga
      361 acgcgcgctg gcaccagtaa ctcctcgggc gaggccgcca gccatgcggg aatgttgagc
      421 ggcagccgat agccggagtt gttttcgaat ttccaaatga gcgcgtcgtg gccgcggggc
      481 actggaatgc ggattacgcc tgggcgtcga gtaaagcggt gtgcaatcgt tccgtcgggg
      541 ccgcgcagca cccctgtgcc gccgctggag taaaggacaa gctcgcgagt gccagcaggc
      601 acgtagacat acatggtcca cgagtgtctc agcgcgggca aagctcgtgc tgttgcggcg
      661 atgacgattg gagttccggc ggcccatgag agatccactc cggaattggt atcatcaacg
      721 tcgagttcgt aatgaccggg cccgggcagt tgaaaggaaa cctcatgatc cgcgcgatcc
      781 gcgggtaccg aggtgtgttg gcattcttcg atggtcgcac ctgatgggca caacgcgatg
      841 cgaacctcgc cgatggcacg gacatctcct ccgcggacga ccaagtggag tggcaccgca
      901 gggtcgagta gcaggttgaa acgctgctgt ccgcgcagcg tgatgccatg cagacctcga
      961 tcgttatgga gatcgtgtgg cgagagacga actaggctgt caacgcggtc tagctgtctc
     1021 gccggaggca gcggtgcacg ccgcgtagct gcacgaatcc agctttggat ctccgcttgc
     1081 gtgactagcg tttcgtcgcg ccatggctcc gccgctgtgt ctcgccagag tgccggatcc
     1141 ggtgccacga tctctcgatc gcgtatcgat gcgtctcgcc gcagggcgaa tgagtcgacc
     1201 atgtgcgtac ggcgaatgcg gtagctatac accattagtt tttcgaaggc cgactgccgg
     1261 ccggagcctt tttcgaacgc gtagtcgatc cataggtcga ggtagtgcaa gtactcggcg
     1321 aggacatcaa gccgtgcagc gactgcaggc ttcggttcga ggactcgagc ccgctcgagc
     1381 agtcgataca actcgccaac gaactccgag cacaggagcg gatgggcgga ttggtcaatg
     1441 ctctcataga accggctcat cacgtgtcga gccctgccga acgcgtggct caggaaatcg
     1501 tccacatacg aactcggtac cggcgagcga tcggcattcc agagtgtggc cgcggcgatc
     1561 caatagccga gcccgttcgg cgcccagcca tccccgatct cggcgtcaaa cagctgcgca
     1621 ccggcggaat gataggtttc gagcgaggca acgatttggc tgggcgagct ggaggcggcc
     1681 tgcccaggca ggtcgcgatc ccaagcattt acggaatagt aatcgtgcac tcccatccag
     1741 gtgccaccgg gtctcagacc tgcggcctgc caagcacgaa atgtctccat gaagtcgagg
     1801 cccgatcgtg aaaatgcggt cgctacgaag accgcgacgt tgggctcaat cggcagcgtc
     1861 gggggagacg agtgttcgta ataggccagc atcccgacga agtgcggtgc tccagacccc
     1921 gcttgcaggc tgcgcgcaac ggcgttggct agagtgaccg cgcgattggt cgggcttccc
     1981 aatttggtgt ctcccgggca gccctcccat cccccttgat cactgggttc cattgagatg
     2041 gagatgctgt cgggatgttg agcgaagtat tgcgcggcgt atcctttgac cagctcgacc
     2101 aatccgggcg ccgtcacaca aaatttgggc gatttccctg cgcgcccatc acccaaatat
     2161 tccggatgag ctgcaaacgc cgcaggattc gcagcgacta ttttttgata gctgtggttc
     2221 gtgactatct cgagcccgcc tagacgattc tctcgcgacc actcggcgaa ctcggccttg
     2281 ttctctcggt acgctcccgc accgagaaag agccgacgtt gccggaacgc agcgcgacac
     2341 gttcgctcgg tttgaatctc taactggctc gcgtgcggta tcacctgcca agttgcgccg
     2401 ggaaagtagc ggcggtaacc gacttgatgt aggaagtccc acactgcgct gagaatttca
     2461 tgatcggtag cggccaagat tagaagtcgc tcgggctccg aacggatcac gtaagtttcg
     2521 gcagctacag gggcggatgt cacccaactc gagacgtcgc cccggccgtc gctgttggtt
     2581 tcaagcttga tctgcgggag tttggcatca actccttgcg gaaccctcac atccaagtgc
     2641 cggcccgtaa tttgtccgag gtagtgggcg agtgtgcttg cggcgtccag tacggtcgtt
     2701 gacgcaccgg gtgcaactgc cacgaataat ccgggccgag agaatgaaaa acgctgccca
     2761 taggacgtga tcggagtgag agccggaacc cagcagccac gagccacgtc cgctcgctgg
     2821 tcttcgatct ttgaagttgt ggtcccgttc gtgctggcgc tgtcagcgaa agtccgtcgc
     2881 gcatagcacg tgcttgtcag cgctgtactg atcacgagag gcacatgcca gtagcgaaag
     2941 cgcccctggc tgggaggcaa ggcagaggcg gactgccgga ggcaacagga ggccccttgc
     3001 acaggttctg gatctttgcc gcgattcgag ctcttcgtcg cacatagcgg atcccgaaag
     3061 cacagacgtg agtgggcgtt tgaccaatta cctctcattg ctgggctctc gttgcgtcgc
     3121 ctcttccacg cgcgccggcc ccagcctgga actcgaacgc tgcgccccca tatcgtccat
     3181 gtccggcata gccgggacgc tcgaaccgga ccagaccggc ttggccgggg ccatgcgctc
     3241 cgtggagtcc aggatagtga ctcgccttct cgcgtcagcg gctaactcgg gctccccgac
     3301 gtgttccgcg gcgagtgcca ccgctctcca ggcgccggct gacggcgcat cccgagcagc
     3361 tcgctggtag tgggtgagcg ccatgttcca tctgccggaa ctggcaaact tgtcgcccaa
     3421 cctgcgatgc gcagccccac accgctcgag gttgacgcaa gacgcggcga caaactcgcg
     3481 agtggcgtcc agaagacgcg ggctgccatt gtcgagcgcc aggtcgaaga gcgccgtggc
     3541 gcatttcaaa tcgccaggga accgagcgca gccgctggcc agcttgttct ctgcatcgag
     3601 cggatttcct tgctcagcca acaatcgcgc tgcagcaatc aagctgcggg ggctgtccgg
     3661 caccgccccg tgttgcaggt gctccgccac ctgggtgagg cacacgtcgc gctgcgagcc
     3721 gcataccgtt ccgccctcac cacgacccaa gtcgtcgagc aactccgtgg cgagttgata
     3781 gtgcacatca ggctgttcag aatggcgcgc aagggactgc tcgagcaatt gaacacggag
     3841 cgcgcggtcc gtagctcgca acgccagctg tgctaggacg gctgctcccg cctggccatt
     3901 gggcgccaca gcggttagcg cttcaggcga aaccccccaa cccgaggcca ggttgagcac
     3961 gcccggcgtc tgccgcggat ccagctcgac gcagcgctgc agcgcaccca atgactgctg
     4021 gattgcaccg cctgatttca ggactttcgc caattccaag tgcacttggg cgctctgagg
     4081 ggctcgttcg agcgcgcgcg tgatccaggg cagcggatcg cgacccacca tcagagaggc
     4141 ggaggatccc agcaacggaa aatagggttc cgccggatag gccagaaccg ctcgccccag
     4201 ggcatcccag aattcctgcg ttggtgcccc gtgtgcggcc acgagctcgt cgtgcagttc
     4261 gtgccgcagc cgcgatgggg cgactacgcc gtacctcaag gagagcccga ggcacaatgc
     4321 tgtgagggcc gtaccaaaag ctaggacccg ccgagcactg agccagcgcg gagtcgggag
     4381 gctcgaggga gtgctcccgg agagagcgcc cagcacaaac acaagcagcc ctgcgtatgc
     4441 cggtaactcc aggccgaggt cgacgacgtt ctgaatgagc acgacggcgc acccaattaa
     4501 cgcgcagcga cgtgtgggaa cgctccacgt actcctgcac acaattggcc tcagcgacca
     4561 gaggagcgcg gcaagagctc cgattgcgac cggcaccccc cactctgctg accactggag
     4621 ggggaggttc tcggcgtgtt cactgattat ataaggtccg ccggtctggt aagtggagct
     4681 gatcgaagcg aatgcgccgc ggccgacgcc aagcagaaaa tggtccttcg ctagttgcca
     4741 acttcgagaa aagagctcga ctttgctgag cgaacggtcg tctagcccga gcggtgagtg
     4801 gcgggccgcc caggccaata gcccgccagc gactccgatc gacaccacca ctgtcgcctg
     4861 gagtaagggg ctcagctccg gagcacgctt cggattgcgt acgccccgcg gcagggcgag
     4921 gactaggaac aggccaatag ccaacgcgcc ggacgcacct cgagattcgc atagcaggac
     4981 ctctgcagct atgaacgcga ttccgacgcc gaagagcgct gcggtggggc gagctcccgc
     5041 gcggaacgcc aagccaaggc cacagaacag gcccaagttc aggtagcctc cgagactgtt
     5101 ggcgttcagt aggggggcga tggtgtaagc gtccaccggc tcatagattc cgaagagtcg
     5161 gcgggcgccg acgagttgat gtgcggccgt gaccacggca acgaggagcg gtactgcaaa
     5221 cgcaatcatc aacaggcgac gcgtccccca atcacgcgag agcctggccg aaacagcgaa
     5281 gatcattccg tagcaggcaa ccttcagggc ctccaacacg ctccggcctg gtgccagtga
     5341 tatgctggcc aactttggtt cgggtaggcc gaaggggcgt agggcgcgtg cccaaatgtc
     5401 ggcgttatcc gcagcgatct tctgcagcca aggcaagggc agccgcaccc actgcagcag
     5461 gcaaatcaag gccaaagcac cccacacggc gatgcagaca aacgctcggc gatcgaagta
     5521 atgttgtgta tcatcgcgtt ctttctggag aagaacgcag gaggcactcg cgatcacgag
     5581 gagtgcgaag ggcgcgagga actccaaatg cacagcgccc gctcccagcg cactcaagcc
     5641 aagacccagg gcgagcgtat aagcactcga aagttgaagc gtcagttttg acatcggctg
     5701 gccgaattag acgcagaatg ccgcagccgt actgattccc tccgcagtgt ccctcgaact
     5761 ccgcccacca tcagccgcaa agcgttcacg cccagcatcg acgccccaac tacttagcat
     5821 gaatcgtcgt gaacgctgga cgctccgctg caactaaact cgcgaacggg actaaatgac
     5881 agcgatcccc aacctaagcg ccaacccacg ggcagcagtg cccgttcgca aggacacctc
     5941 caagaccttg ctagatgcct ggaaggtact tcgcaagcgc tgggcatgga tggtcacact
     6001 ggcgctcgca acgattgggg ccgctggatt ttatactgcc gggctacaac ggatttatct
     6061 gtccagctgc gtgattgaga tcgatccgaa gccgcccaag ccgttggggc aagaagtgca
     6121 ggcgatcgtc gatgtgggca atagctcata ttgggcgaac agcgaataca acaagaccca
     6181 gtttcagatc atacagggcc gggccgtggc agaggaaacg gtccggcgtc tgggccttca
     6241 cagaaacccc gcgtttctgc agggcctgag tcccaatcag aagccggcgg acggcgcggc
     6301 gcaggaaacc agcgtcgcag cagcagctca gatggtacgg ggacacctcg tcgtggagcc
     6361 gatccgcgat agccggctag taaccgtttc ttaccgagat ccagatcccc aacgtgcaca
     6421 ccagatcctg tctacgttgg tcggggtcta tgtggaccga aacatcgatg tggctctcga
     6481 ctccaccagc agcgctgcgg agtggttgcg aggtcaggtt gacaacctga agcaagaact
     6541 cgatgggtcc gagctggcgc tgcatgaata caaggtctcg aatcgaatcc tctcagcctc
     6601 gctggacgat cagtcgaaca tgctccggca ggagatgcag caattgagct ccgcgctaac
     6661 ggccgtgcgc gtccgccgca ctcaggtcgc ggctcaggcg cgggagctca cagactttga
     6721 aaggcagggg tcggagtggc tgccgaaaga tgatgtacta gcgagtgaga ctctacagcg
     6781 gctgcgcgag actctgagtc aggcggtggc ggagcgggac tcattgattg gatcggggaa
     6841 gggggagatg catccgctcg tcgtcgctgc cagcgcgcgg gtaaatgcgg cgcaagacgc
     6901 tctacagcac gaaatcaaga acgtgaggga agccgtccag cgagatctgc gggtcataga
     6961 tcaagagatc gcgagccttt ccgagttgtt tgaacaggcg cagggaaggg cgctttcgct
     7021 tggaaagctg gagattgagt atcgccgact ggatcggaac aagctgaata cggaaaagct
     7081 ctattcgatg gtgctcgagc gcagcaagga gagcgatttg acgctcatga tgcgctttaa
     7141 taacattcag gttatcgagc cgccgcaggt tccggaacta cccgtgagtc cacgtgttcc
     7201 cctcaacatg ggcattggcg ttctgtgtgg gctggcgctt ggcctgatgg gagcgttcgg
     7261 ccgcgaaatg tttgatcaaa gcgtcaagac cgccgcggac gtggaggatt tggggatcac
     7321 gcttcttggg gtcgtgcccc tgctaggcga atcacgaagc gctacttatg gacggaggca
     7381 gaaagcccga cgacggcagc agcaggatgc gcgtaccgag ctgattgtac atgcggactc
     7441 caccaatgct gtggcggagg ccgctcgagg cgtccggacc aatctggcgt tcatgtctcc
     7501 agacaagccc ttccggagtt tgttggtggc gagcggtggt ccatccgagg gcaaaaccac
     7561 ggtggcctgc tggctcgcga tcgtcatggc ccaagctggt cataaggtgg tcctcgtgga
     7621 ctgcgatctc cgacgtccac gcctccaccg agtgttcaat ttcaccaacg accgcggtgt
     7681 gaccaacgcc ctggtggagc gttcgactct ggcagatgcc attcgtccca ccgaggtacc
     7741 agggctatcg gtgctgctat ccggcccgag atgcccaaat ccggcggaga cgctgcaaag
     7801 taagagtttc gagacgctgc tcgcggacct cttggccacg tacgatcgag tagtaatcga
     7861 tagcccaccg gttggtcctg tcactgacgc cgtgattctt tccacgcgcg tagatgcaac
     7921 gttgctggtg gttcgagcgc tgttttccgc gcgtgacatg gttcggcagg caaagcgctt
     7981 actacaggac gtgcgctcca atctcgccgg ggccgtattg aatgcggccg accaacatcg
     8041 gcagggctat ccatattatc gctattacgg tcgccatgat gcggacgaag caccgtcgca
     8101 ggaggatgct acgtgatttg gattcttttc gcgcaacgct gcgcgtccaa gaagttcaag
     8161 atcgcctgag ctctcgtcgt ccatgtatag aatcggttga aatcgtagta cgctgcagca
     8221 gacagtcggc gactgagttc ttcgtcctga gaaagtcgct cgatggcgct ctgccaatcg
     8281 cgcacgtcat ctgccctaca gactaatgcc gtgcgctcgt gctgcactat ctcgcggagc
     8341 acgggtaggt ctgaaaccac gagcggtttg cgttgcgcca tgtactcgaa gagctttagt
     8401 ggggacatcc agcgagaggt atctcctgct ccgcctgaga tagccacccg actctgatac
     8461 ggcgccaaga gaacatcaaa gctcgcgtaa tgtcgcgcga ggtccgcgtg gcgaacatag
     8521 ccgtggaatc ggatgttcgc ctgctcgcca gcgctctggc tccagcgacg taaatcagcg
     8581 tcggtaccac ctaccaagtg aaaatcgacc gcaggcattc gttgagccag ctcgagaatg
     8641 agctcgatgc cgcgtccggg gtacagatgg ccaacatatc cgacttgtgg gcgaccattg
     8701 ttctgtgcct ttcgcccgaa ttctgtgcgt tcctcgcaag ggggatccgc gccgtcgtgg
     8761 gccacgaaga tgtggcgctc cgctgttgga aaaagtccta ggaagtgctg cttcagcgca
     8821 tccgagatga cgacgatgcc aagaaaggct ggcatcctca agagtctctg gatcagggtt
     8881 gcttttaagg cattctcggg cggagtgtgc atttccaatg cggccaggga gcccatgctt
     8941 gcgaggccga atatgctcgg caagtggcgg ccgtatatca gatcggggcg actacagtga
     9001 gcgccagcca ttacctgcaa cgcaaatgtg ccactcccaa tgccaaatgg aagcgggggc
     9061 caccgcgcgg cgtggatacg gaagacgggt ggcactccaa agtctcggaa ggcactggaa
     9121 gccaagtgcc gcgtcgtacg cgcccataga tcgacttgat gcccttggcc ggcgaaagct
     9181 gcgcacattt tcatcacgtg cacggtgttg gcggcacggg atgggatttc ggacccagag
     9241 aggtaggcga ctctcatgga ctggctgctc cccgaagagt ggagtgttga tcgaaattgc
     9301 accgatgcaa cgatcgtccc cgttgctggt tgtgaaaacc ccaaatgcgc gcgcaaaatg
     9361 cgagcccatc ggaacggcac gacggcatag actcaggtga tctatgatag caaccaccag
     9421 caatcgtcat gatacctcgg caaggcacac ttgattgtag cgaagcggtt ggccgcgtac
     9481 ggctcggagg atcagctcgt ccaatagcgg tctcccaagt ctcaaacgga tgggcaactg
     9541 gcgaatctgg cgggtgactc gcaagccccg tgactgcaag tagcgtttga actcctcacc
     9601 gttccattct cggacgtgtt gtgggttggg agattgtgtg cagttcggtc cgcgcaacaa
     9661 gtcacggtct ggcgtcgaaa tgatgagtag tccgcaaggg gtgacgtgct gcttgagata
     9721 gtcgagcaac acatccggat ccagcacgtg ctcgattacg tccgcacaaa ttacgacgtc
     9781 tccttggatg tcactcggag tctgcttggg gttcgcgagg tcttcttcca gccagtcccc
     9841 ccattggtag tgtccgcggc agtaagtcac ggccgacccc tgatcgatcc caatgaacga
     9901 gacgtctggg ttgcgttttt gcagcgcggc caacttgggc gctggcccac atcccacatc
     9961 gacgacagtt cgagctgctc gtcgccgcag ttcgcgctcc gcgagcgcgt acacgcccca
    10021 ttgataggtg tccgcggcgc gaatgcgcgc tgccgtccaa taggtttccc cggaggtgcc
    10081 ctcgtgggtg agcgccgtgc tccgagcgat gtattgggtg cgaatgcaga aattgtcgtg
    10141 tgccatgtct gtcaaagcgc gtagagcagt gttgttgtca cccgttcatc cgcagcacaa
    10201 cggcggcggc gggcatccgc taagaggagt cgcccagcgg attccctcgg ctgccgggtt
    10261 gtgctgccgc cgtggcggaa ctcaactgca cgctcgcttc ctggcgagac tttgaccata
    10321 ggctgaactc agcctgcagg accaacaaca tcagtgctgc ccagatggta ttgaaggcga
    10381 cgaatgctgt agcggcgcca atggcgccga cgattgatgc aagccaaatt aggaggggca
    10441 ggtatgtcgc ggccgccgct gcgtgcactg cgagggcggt gggggccttt cccatggcca
    10501 gaagggcagg ggtgagcggc gtgctgaagc cccaaagaac ccaacctatg ccgcaaatgg
    10561 cgaacactcc aaatgcgtcc gtaaactgca cgttcaacaa gtgtgcaatc atcggtttgg
    10621 ccaccaacag accaatcagc agcgctccgg cagcactgag ccctgccgcg accgcaccaa
    10681 acctcatggt gcggcgaaat gctgaaagat ccccggctac aatgatgcga ttcaactcag
    10741 ggtagagaac ctgatagaag gggtcggtca gcaccgaaag gaacgatgca aatttgtgac
    10801 ccgctttgta gactcctgcg gcggaggccg agctgaaggc cgcgaccacc atcacgtcca
    10861 gctcgcgtga tactcggacg gtgctattca gatgggaact gagcgcaaac tgtacaaggg
    10921 agcgattgcg ctgaatgtgt ccccgggcgt ttgcggacca gattcgcgct acgccttccg
    10981 ctcgcaggcg aaggcaactc gcaacaagaa gaaatagatt gctcgcaatg tccccgcata
    11041 agctcgcgat cccgaagccg agtacactgc agccaaaaaa aagagcggtt gcacatgcaa
    11101 gtagtcgcgt cactgatgcg gcgagatttt gtagcgccag agtaccgaat cgatcgtaca
    11161 tgcgcaaaat ggcggtggga gtgccgccga gattcatggc gttgctcgcg cacaacacgt
    11221 aggcgagccg cgtggtttca ctgccccaag cttgccagtg agcgatcagc gtaacaccaa
    11281 cgcagcctaa cgccgcgctg gccaaggaag ccgcgccatc cgacagaaag cccaacttca
    11341 gacagtcccg caggcgatcc gggtcctttc ccagatgggg tgttccgtaa cggatggtca
    11401 tttcccacga ttggaacccc acgattttat gcaccaccag cgcataggcc tgtgcagtcg
    11461 ctagttgacc cagtcgggcc ggccctaagc cgcgtgcaac caggagaaaa gccgcgaacg
    11521 acatcaggac tgaggcgccg ctggaactcg acagcagcga agcttgtttc agaacgcgcc
    11581 agcgtgattg cacgtagact gcctcggaat ctcgaactca ggagagaccg agcaattcta
    11641 gggtgcgatc ctgaatgctg ctttgctcac caaagtaggc tttgccccat gtggccattg
    11701 gtacactaaa gcctgttttt cctcggcgga gcacagcccg cggcaagcgc tcgctgaggg
    11761 ccgccttgaa tagtcccttg agctcacgcc cacagagcct tatggattct ggcaaggcga
    11821 acatcagctc cacgagctgt atatcaagca gcggtacccg tacttctagt gctaccgcca
    11881 tgctcgcgcg gtcgaccttg gtgaggatat cgtcgggcag ataggtatga aagtcgagga
    11941 actgctgttg agtgaccgcg ggcagatcct tccgatagta tctgcggaag gaccagtagt
    12001 catcgtagtc ctccgggatc gaccacctct cacgataggc agccttttcc tcgtgcacga
    12061 ggccgcctat taagcgggtg tggacggcca aaggctcgtg gacaaatgca gactccagac
    12121 gtcgtgcaag cgatcggaga cgcggatcca gctcgcgagg gatacgagaa gtcagagctt
    12181 tcaggtagcc cgagaatcgc gtgggaacga accggcgcgc caacaggact cgctcgtaga
    12241 gattataccg caaatagcca ccaaatattt cgtctccacc atcccccgtg agcgcgacgg
    12301 tgcactcgcg ccgtgctgcg cgcgagacga gataggtcgg taaagcggag ccggaagcga
    12361 agggctcgtc gtacagtcgc ctaacggtcg catgtagctc tcgagccccg tcagcattga
    12421 ggcactcatt gcgatgccag gcgcccagga acttcgcggt ggcagacgca ccggccatct
    12481 cggagcgctt atcgtcatca aagccgatgc agaatgtccg aggggtagca ccggcatcta
    12541 ccgcagcagc aacaaccgaa gcggagtcta cgcccgcact caagaagaag ccgacgggca
    12601 catcactcac aagttgcgct tgcacggcgc gagcgagacg gtgctgaagt tcctgttgcg
    12661 cctccgcccg agtgagcctg agagaccccg gttcgggtgg atgccagtac cggctccgcc
    12721 tagtcgtttt gttcttcaga tccagttgta ggagttctgc gggctccagt ttttggatat
    12781 tcttgtagag cgacttgggg ctcggcacat acaagtacgt gaagaagtcg tacaaggcgg
    12841 tgacgtcgag ttcgagcttg ccgttgaaat gtctttgaat ggcctttagc tccgaagccc
    12901 acgcaaagac gccatcgcac caaccgtagt acagcggttt gataccgact cggtcgcgga
    12961 ccaagtaaag catgtgctcg cgggcgtcgt agatgacgaa ggcgtacatt ccatcgatgc
    13021 gtgcacagag gccttcgatt ccccagtcct ggtaaccgtg gacgagcacc tccgagtctg
    13081 agttggaccg aaatcggttc ctgccgagtt cgtctcgcaa ttcgcgatag ttgtagacct
    13141 cgccattaac cgccaccacg acgctgccat ccggagagac cattggctgg cgtccgcgct
    13201 cggacaaatc gagaatgctc agccggcggt ggccgatata gacgcgctcg tcagtccact
    13261 cgccggcgct gtcagggcca cggtgccgga gagtatccaa gctgtcgcgc gagtgttcct
    13321 tggagtaact ctgcgttact agcgtgtgcc cgaaaatccc acacatccac gcaatgctag
    13381 cttccgcgct gggcacatgc aacgctcgct gagtttcttc cgagtcgtgt ccggctatgc
    13441 gggtctgagc ttgagaagca cttggtcacg gagcagtggc tcgctctccg tggcctcggc
    13501 aggcaagtag cgatggtgcg tcgtccatga ctgaacgttc cggagacgtg gactgagtgc
    13561 gcgggtcttc ggggatgatc tcgttgaccc gtgtcaacgt cagccacggc ttccggtttg
    13621 ggtagccggc tgttgcggtg aattggagat cgtgtctgcc atctcgaccg ttcgcccgcg
    13681 cgaggaatag ccagcatcga agagcgcggc gtagttgctg acgtttttcg tcatggaaaa
    13741 ttcgcgcagg acgcgcgctc gcgcgagcgc gccaagcttc tcgcgctcct ccctggtccg
    13801 gcccagtaag tcagacacgg ccgaccctag tgcgaccgga ttagatgaag gaaccacccg
    13861 ccccgtgtcg ccaatgatcc aggctgtgtc acccacatcg gtggctacgc atggcacacc
    13921 gcaggccatg ccctcggcga tagcattggg aaagccttcg ccaaacgcgg acgacagcac
    13981 gtttacgtcg aacattgttg cgcaccggcc tccatcgggc acatcaccga gcaaacggaa
    14041 gcggtggcgg agccctagct cctcgatttg catccgaagt gcgtcgttcg accagtctac
    14101 gccgcgcccc atggcaatga agcaaacggt gggaaactgc gcggcgatcc gcgcagcagc
    14161 tcgaatgaaa cctggatagt ctttctgcgc gtggtgacgt gccatcatac cgacgactaa
    14221 ggcttgcgcc gaaacgtcgt gtcggttgcg caagctagtc cgtagcgcgt cgtccgggcg
    14281 gagtgcctcg caatcgaacc cattggggat cactgtggcg cgcgtggggg aatatcctcg
    14341 ggcccggtgt tgtgcttctc ctcgatagga gttgtggatg atccaggtcg gtaggtgcga
    14401 gaggcgtgct ccgagactca cggcgagtcg tgtgccgagc ttctcttcca ggcgctccga
    14461 aggagagcac cgcacattcc atccgtgcaa gggcacgcaa gcggcggtgg ctgccagcgt
    14521 tgccgccaaa ttgccgtggt acatccagcc ttggacgaca tctggacgcg agcgtagaca
    14581 atgagcgatc agggacatca acgtggcgct cagaccccct cttcttggcc cgcagttgag
    14641 cgtgcggagg ccttgttccc gcatttgacg agctaccaac ccatcgccaa tcagcgagaa
    14701 ggcttccgag ctgaagcgac tcttgtcctc gtgcaacatc aggcgcatca gcgccctctc
    14761 cgcgcctccc tggttgagtc cggtaatgac gtgcagaacc ttcagcatcg gtagtctaac
    14821 cattgctgac ggccgttaga aagcgttacc tcagaccaat tcacgagcca ctcgagaatc
    14881 gggtgctatg agcgtcgctc tggggtcgca cttttgtcta cacgccgcac cggggatcac
    14941 gggcctggac ctatcctgac acacgatgac tgagaacaag tgccgcttgt tgcgcgccac
    15001 gtggatcgag caaatgaacg gcgaagtcat cgccatcttg gctacgttga ccttgggagc
    15061 ggctttgcgg cacgcgatgg cggcagtgcc acctttgccg gaggttgcct atcgtaacag
    15121 cgtgatcttt ctccaggcac tccggcatcc tggctacgtc cttgcgttac tggcgggttc
    15181 gagcgtctac atactgacac agcgagggcg ccaaatcgtg cgttggtcga tgctcgagcc
    15241 cgttcacctg cgctggtttg tagtgctcat cacttggccg ctagcctggt actacagcac
    15301 gtacgactac aacttctact ttgatcagac gcacgggtgg gatcgcgctc tgttggtgct
    15361 gctgtggctc ggtgttcttg caagtccggc gctgctactg cccttcttgt tacagtgtgt
    15421 gatcttgatt cgacagttcg actatccgga cgtcctccag tacagctgga cagataagag
    15481 ccttccgctc gcctttgcgt gctgcttctg ggcgtactgc attgtggcgt gcttcactag
    15541 acgtgcggag ttgaagcggc tcttgatcgt ggtcatggcg atcttcgcgg ctcactactt
    15601 tggtcctggt gtccagaagg tgtcgttggg agaacatcct ctcgattggg ctttgaacaa
    15661 ttcgctgcat cacctcttcg tcgcggcgac tcgcagcgga tggttggaca gggtcgatcc
    15721 gatgtcggtg atgagcatcg cccactggct cgagcgtgcc accattcccc tgcaattggc
    15781 gactcttgca ttcgaactcg gtgcggtact cgtgttctgg gaccgtagag cagtgttggc
    15841 tgcggcgttg ggggcggtct tcctacatgc ctccattttc ttgtccagtg gcattctttt
    15901 ctggaagtgg gtcattgtcg acctctccgt gctggcgttt gtgggtgtca tttggcggga
    15961 gggcatgagc cacattgctg cagagcgaaa gcttgcggcg atcgcgactc tactcgttcc
    16021 ccttgccacg gtgatttatg gtgtccccct cctgggttgg cacgacacgc aatacttagc
    16081 ttcctatgaa atcatggctc aggatacatc atcacgacat tttagcctca gtcgacaatt
    16141 cttcgcgcca tatgacatgg tgttctcgca gaaccggttc cggttcttgg cacctcagcc
    16201 cgtgctgccg gtgggtactc tagggtccac gcaggatagc gatctgcgcc gcgagatcgc
    16261 agcggcaacc ccggagacgc tgccggcctt gatcgagcga ctgggtgtta gacgcttcga
    16321 cccggacaag aagcgctcgt tcaacgcttt cttggcagac ttcattgatg gcgccaaccg
    16381 gcgtggcatc caccgcgtgg ttccagaatg gcttcgggct ccgtctcaca tctggttgga
    16441 aggcggagag aaccctcaac ccctcgccac gcccatcact catgcgtcgg tcgagttccg
    16501 cgaatatctg ctcgatccct ttccttcaca tccctatcga cgcgcggtgc tggatgacgt
    16561 ccacattccg atttctctca gaggcggttt ctcgaggtga gcctcgctgt atcagaccac
    16621 tcacaggacg gcgtgaccac gcgcttgacg caactttttg aacggccgct acgagcggcg
    16681 caatttccat gcgcagaata ctgatcgtag ccgggctagc ggaatccctg gtgcgctttc
    16741 gcggagatct aattcagtct ctattagctc gtgcgatcga ggtgcatgta gtggcgccct
    16801 gtggcgtcca acctcggtct tgggaaacac tccggtcttg gggcgtcgtc ctgcaccaga
    16861 tgtctctcga cagaacgggg ctaaatccgg cgggcgacat gcgggttatc gcagagctgt
    16921 atcgactcct gcgccgaacc cgccctacgt atctattggc ttatacggct aaaccggtcg
    16981 tgtacggtct cttggcggcg cggttggcgg gaattcagcg aacaacggct ctcatcactg
    17041 gacttggcta cgccttctcc cccgaaagcg gaatccgaag gagggtgatc agacatttgc
    17101 tcgagtcgat gtatgggttt gcgcttggtg gggcccatcg tgtcgtattt cagaacccgg
    17161 acgacatgcg gctcttcctc gatctcggcc tggcgaagcc tgagcagtgc gggctcgtga
    17221 atggctcggg tgtcaatctc gagaaatttc cggacactgc cctgccgccc ctgggctcga
    17281 gcatgcgctt cgtgctcgtc gctcgcctga tccgtgacaa gggcatctac gagtttgctg
    17341 aagccgcacg ttcagtgaag cacatgtgtc ctgagacgga gtttcatctc gtaggaggac
    17401 acgactccaa cccggcagcg atctctcgtg cggcgctcga gcagtggatt gcagaaggaa
    17461 gcatcaccta ccacggccag ctagaagatg tccggcctgt gttagcgcaa tcccacgtat
    17521 tcgtgttgcc ctcctactat cgagaaggaa tccctcgcac tgtcctggag gcgatggcca
    17581 tggggcgtcc tatcatcaca accgacgcgc cgggctgccg cgagactgtc gtccaaggcc
    17641 agaatggcta tctcgtcccg gtgcgagact caggcgcgct tgctagcgcg atgttgcatt
    17701 ttgtcgagcg accggagttg attcaaatca tggggaccgc ctcccgcgaa atggccgtcc
    17761 gaaaatacga tgtggccaag gtcaactcag ccatgctggc acacattgga gtagacggtt
    17821 gagtgcgcgg cacatcgttt cgcgccggaa tcgacatggg gaagccggaa gagaatgact
    17881 tctgtcggtg tgttgttcgt ggtgctgggc gctggcctgg ggctctggtt ttccgtgaaa
    17941 tggcgctctc cagtctattt ggccttgttt gtctttcatc tcatggcctc cctcttcttc
    18001 tggaactaca gcctccatca tcccgccgac gcggtggagt actaccgcta tgtcaattac
    18061 tactcagaaa ttgccatagg caccacgctc gtagcctggc tcactgcaac gattcgggat
    18121 gctctggtgg cgagttacct agacgtgttc atggcgttcc atctttttgg atatttggca
    18181 ctcgtgatcc tgttcaggct ctgcgcggcg gtgatgagtg atttccagga gcccgacgcc
    18241 gtggatgggc aacagagtct tggcaggcgc gccttgctgt actccatgac gttcttgccc
    18301 ggccttcact tctggaccag tcccatctgc aaggatggcc ttgcacttct cggcattacc
    18361 tctttcacgt gggggatggt tcgtcccgag cgaagattgc ttgcgctggt gttcggctta
    18421 tttgctgtag gcatggtccg cccgcacatt gcggcgttgc tcgcggctgc cggggctttg
    18481 gcactggtct ttagcaggga cagttccgcg ggccttcgtc tgctactttc ggtgtcattg
    18541 ctggctggta tcgcagtcag tctgccattc ttgcaaagct acacgggatt ggatgagcta
    18601 acggcgcagg gggtgggcgc atacgtagac caacggcagg agagcaatct ggtcggcggc
    18661 tcagtggtag acatctcgca gtactcgttt cccatgcagg tcttcacata tctttaccgg
    18721 ccgatgtttt tggacgcgag cggcgcgttg gggctcattg tttccttcga gaatctttat
    18781 ctgttcgcgc tctcgttgtg tctgcccttt cagctgaggc ggctctggcg ttccttcgag
    18841 catcaggcgt ttctccgatt caacgtcatc tatttctgtg cggtgacggc catactggcg
    18901 agcaccacgg caaacatggg gctcgcgttg cgacagaaga tgatggggtt gcccagcctg
    18961 ctcgtcatgg tgctcatgag cttgtcagcg cgcgagccgg tcgaggaact gctggcagat
    19021 gaagctgtgt cagactgagc agaaggcggg gactacacaa tgaacacaca tgagaggcgt
    19081 gctcggcggg tgaccgagta ttttgaaaat cccagcctct atttccgtgg gcggtacgtt
    19141 gtggacctcc gtaagacgct cgctcgcgac ctattgggcg agctgaagga gaccaggatt
    19201 ctagatttgg gctgcggtga cggtacggtg tcgttgcagt tcctgggcaa tggcaatcag
    19261 cttaccctgg cggattcatc tcgagccatg ctgagtctgg tcagggcatc tgttccgaag
    19321 cacttggaga acaccgtcac cttggtggaa tcggccctcg aggacttgcc ggaccagcaa
    19381 tttgacatcg taatctgctt gggggttctg gctcacgtgc agtcggtcga gcgcgcggtg
    19441 caaaagatcg ccgcgagcat gcggccgggt gcgagatgcc tcattcaaat caccgaccac
    19501 gatcgtagga ttgcttcgtt ggtccgacta tttgcaggag tcaggcacct tcttcagcaa
    19561 tgcggtcggt atgaggtcaa cgtgacgacc cgggcgcaac tggagcagtg cgctggaaac
    19621 ctgggcctgg agtgtctgga gtccaaagca tactcacttg gtattccggg cgcggggcgt
    19681 ctactttcgc agcgtgtgtt gtacaagtac gcggagttgt cgctccgcag tacaatgatg
    19741 cagccgttgt gctctgaggt tcttctgctg ttcaagaagc aggttggaca tgcggtgctc
    19801 gctaccgccg ggcgcggtaa cgactattga attcgaggag tccgtacgtg gagcgctcgc
    19861 atctagagaa gctggcgaaa cgcacgctgg acgtgggggt ggcagggatt gggctcgtca
    19921 tgtctttgcc tctcctggtt ggggtgggga tcgcggttcg tcgcgaacta gggtcgccgg
    19981 tcctcttcag gcagatacgt cctggattga aggagcgacc gttcgagatg atcaagtttc
    20041 gtacgatgtg tgagccgacg ctcgggcagg atcgcttgga ttcggatgca gaacgaatga
    20101 ctccgctcgg caagtttcta cgcgcctcta gtctcgatga gttgccaacg ttgtggaacg
    20161 tgctcaaagg agatatgagc ttggtggggc cgcgcccgct gctgcccgag tacttggaac
    20221 tctacagccg ggagcagcgt cggcggcatc tcgtgaggcc gggcgttacg gggtgggccg
    20281 tggtcaacgg ccgcaacgcg ctcgactggg acagcaagtt tgcgctcgac gtgtggtacg
    20341 tcgaccactg gagccttggg ctagacctga agattctggc gaagaccgtc gtgcaaatcc
    20401 tgctccgcac caacgtggtg catgaaggtc atgcgaccat gccccgtttt cgcggcagtg
    20461 tgatcaatga ggctgacgac tctacgactc ttcaagaacg gcgggaaccg tgatactggc
    20521 ggacgcaggc gcgttgacag agcgcctcac ccgtgcttgc tcacgggttg cacgctgcga
    20581 gagcgacgac cttccgcgtc gcagcgaggg atacgaagca cagtagaagg tgcctgggga
    20641 tggcgccttc cggatgaggc aaatagcggc gtgcaccagg gcgctcaagt gccagttgct
    20701 gcttcgtgtc aagtgaggct gagcttgggg ctccgggacg ggtgcggccc ggagttcggg
    20761 caagaatgtg tggacggcgc atttccaggg ccgccgcggc acgcggtgtt agtactcacg
    20821 cgctgcgccg tcatggtgct ccagagtagc gagggtcgga acttgcgcta ttacgttggg
    20881 cagtagactg gcatcagtct ccggggccat cggcgccggc tttcggtaaa ggatcaatcg
    20941 cgtgggccta ttagaacagt tctcggcaaa acagggccac atcgtcgtgg tcggtgtcgg
    21001 ttacgtgggg ctaccgttgg tagctgaatt cgttcgcgaa ggttttcagg tcacgggtct
    21061 ggactatgac gcgcgcaagg tcgagctgtt gagtcagggc gagtcgtaca ttcgggacgt
    21121 gccatcttcg gatctggctg cgcatgccaa atcggggcgc ttacgtgcca cggctgatcc
    21181 ggcggtgctg agggaagccg atgccgtcat cgtctgcgtg cccacgccgc tgaacaagac
    21241 gaaagacccg gacatgcgtt tcatcgtgga cgctgccgaa cagattcaaa agcaccaaca
    21301 cccgggcatg cttgtggttc tggagtccac aacctatccc gggacgacca cggaactgct
    21361 ggttcccaag ctgacggagc gcggtttcga gctcggcaag gacgtgtttg tcgcgttctc
    21421 gccggagcgg gttgatcccg gcaatgagac gttcaagacg cgaaatacgc ccaaggtgct
    21481 gggtggggcg accccgcaat gcctggcggt cgccagcgcg ctctactccc acgtgatcgc
    21541 gaagctcgtg ccggtgagct cgacggagac ggcagagatg gtcaagctgc tcgagaacac
    21601 ctttcgcgca gtcaacatcg gcctggccaa cgagatcgcg ttgatgagtc gcaagctcgg
    21661 cgtggatccg ttcgaggtga ttcgtgctgc cgccagcaag ccgttcggtt tcatgccgtt
    21721 ctatccgggg ccgggcctcg gagggcactg catccccatc gatccgctct atctctcttg
    21781 gaagctcaag tccttgaagg gccaggcgcg tttcatcgag ctcgctgact cgatcaatgg
    21841 ctcgatgccc aactacgtcg tcgagcgcgt ggcagcagcg ctcaatgagc attccaagtc
    21901 ggtcaagggc gccaagatct tggtctatgg catcgcctac aagaagaacg tcaatgacgt
    21961 gcgagagtca cctgcgttcg aggtcatcga ggagctggag aagaagggag cacacgttgc
    22021 cttcacagat ccgctcgtcc cgacggtcga gttgcccaac ggtgaatcgc aggcaggagt
    22081 cgcagctgat tttgcagcgt acgacgctgt cgtggtgatt acagatcacg acgcgctgga
    22141 ccgggaacgg ctggcgagag agggtcgcat catcattgat acgcgcaatg ctctggccgg
    22201 gatcaaggac ctggatcgga agcgcgtctt tggcctctag cttctagcag ccagccgagt
    22261 tgcgcttcag ccaagctggc gcgtcacgcg cgcgggaaca cccatgcaaa cagagttgtg
    22321 tggcacgtcg cgcgtgacga cggcccccgc gcccaccagg cagcgctcgc caactcgaac
    22381 ccccggcagc acgcaagcgc agcagccgac gtggctcaat tcgcccaatc gggctgcgcc
    22441 ggcgagcgtt gcgttgggag aaacgtgagc aaatgctccg acgtgcacat cgtgttccac
    22501 aactgcgccg gtgttgatga tggcgccgat gccgatgcat gcgcacgggt tgatggtagc
    22561 tagcgccaga accacgacac ccggagccag gctggccgtg gctgagacaa ccgcgcgagg
    22621 atgcacgacc gtcagtggtt ttctgccatc gctctcgagg tcggcgcaga tccgctgccg
    22681 agcaaagttg tcacccactg ccagcacgat cgacacccct ggatgatcgc gccaccagcc
    22741 ggagcgccca agcactcgtc ccaaggcgag cgagtctccc acgggcctct gatcatcgag
    22801 gaatcccagg acgtcgagtc cggcgcacct tgcagcgtcc gctacgacgg agccatgacc
    22861 gccagcgccg taaaccacga cggcagaacc ctcagtcatt catcccccgc aaatcccccc
    22921 catacaaacc ccactcgagc actccctccc ctccccacaa acctccccct tcgcccgcgg
    22981 cgcccgcaac gccgaacacc ccagcacaaa cccatccagc caaaaccccg agtgcagctg
    23041 catctgggtg accaccgctc cctccgggca cgaggagcgg taggcctcgg acccgctggc
    23101 agagaagtta tccgcctggg aaatcaacag gtggccggag ctcgaaattg cgaggtcgac
    23161 gccgttcgcc gccagcgtca gcggtgcgca ctcgatcacc aggctgcgca gggccgaggt
    23221 ctcggtgtcg tccggcgcga tcgggtcgta ttgcccggag agcccgacca gcaccggcgc
    23281 ggagtcgggg cagaacgccg gcggctgggg tacccaggtc aggcgcgtgt cgggcacctc
    23341 gaggctcgga acgccgagga agggctcgct catggaccag ttcacgagcg cgtcgtcacg
    23401 ccggatcgtg atggcggcgc cgccgacagt ggcgctcgcg cacaggggtt cgatgaacgc
    23461 caggcgttgg ccaaatgtct cggtgctggg gttggccgtg gctcgaacgc cgaccacgac
    23521 gcccccctgg cacgtgccga ggcgcgggcc gccgccgact ttgcccacca gttcgagctg
    23581 ggtcggcgcg tccaccagga tcggtctcgc cgacggcgcc ggtggcgcgg cgtcgggcgc
    23641 catgccaccc gcgtctgtga tgccaggagc cgtcacgcca gcgtcgctct ccggccgccc
    23701 gacgcccggc gccggcgtgg aggggagggc gccgttcggg aagggtggaa agctcaaccc
    23761 gccctgcacg ggagctggat tcggctggag cgtgtccatc gcttgcctgg ggcgccgggt
    23821 gacctcgacg tcaccgatca gggcttcgca ggcgctggcg ccgagtgttg tgagcgccgc
    23881 catcgccgaa atcgcctgca ggcgcctcat gatgcggcat tctgtgcggc ggaacgctcg
    23941 aattcaagcc aagaggcccg cggaatccgg gctccgcgcc cgcaccggcc cgctgacgtg
    24001 gcagagcgcg gcgcgcgcag ggcgcgtcct gtcgcccccg gcgccgcggc gcagggagcg
    24061 cggcggcgcg gcctcgtgtc tccgtctatg agctcagctc gaacgcaccg gagcgaacca
    24121 ttgcatggtg caacagcgag agctcagagg ttgcccatcc agctgcggat gcagtcgatg
    24181 tcgctcttgg agagggtgac gccgccgagc ggcatggtgg aaccgcacgg tggatcggcg
    24241 gagagcttct ggatcatcag gctgccgtcg gggtcgtccg ggtcgatcac attgccctgg
    24301 ccggtgcagg cgagggtgcc gggcttgtcg atgaaggcgc gcgcggctgt ttcgctggct
    24361 gcaaagttct cgaggttgga gccgtcgccg tggcagccgg agctgccgca gttggccgcc
    24421 agcaccgcga agccgtcgca gctgctgctg gcaggcgagc ccgcgctgcc tccgctgccg
    24481 gcctccgcgt cgcccgtgtc ttctggctgg ttcgctgctc cgccgctacc gccggtgccg
    24541 gtgttgtcgg caggggtcgc gggaggaggc gctgcgctgg cgccgcccgc cgccgaggcg
    24601 accgcgttgg agggcgcgct ggccgcgccg ccgccggagc cgccgttgct cggcacgccc
    24661 ccatcgagct cgtactgggc gcgaatctgc cccgccagtc tggagctcac cactggtgga
    24721 tccgctcccg cgcaggccgc gagcactgcg ctcgagagcg ctgcgcagag aaggagtgtg
    24781 ggacgggcct ggagggttct cgacatcgag ttccatcaaa gcaacgtggg tgccacagcg
    24841 ttaatgtccg gcgccgcgcg cgcgagcaag cgccaccggt agcgcaagtc ttgcgcgagc
    24901 ctgctcacgg cgaaagcgtt gcgttgtcgg catcgccccg gaatgcccca gcgctcgtgc
    24961 ctacgcacga ggcgccgctc gcttcagcgc gaatgcgcac cctgtcgagc gcgtcgcttg
    25021 gcggggcgcc tcggctccgg cgcgtcgagc gcctgcgcgc cacgccccgg gcgcgccgac
    25081 gacgtgccgt ccaccggctg cactgtcacg gccggcgtgg gcagcggagc cggtggcggc
    25141 accggcgcag caggctcggc cgccgcaggc cgcagcgagg gcaccacgcg cacgttgttc
    25201 gcgaggcgca cgcccgccgg caaatcggga aggcccgctc cgtcgcgagc cgccgcgccg
    25261 ggcgcggcga gcgacagcgt gacttgctcg agcgcgggcg cagccgcact ggccagtgcg
    25321 cgttgctcga gcgatgccgt gacgcgcgag acgccgagcg cagcaccggg cagggcgatc
    25381 gcgagcgaga tcgggagcgc ccagtgcttc gcgctcttgc tccggcgcgc cccgtgctcg
    25441 gagcgggcgt tcgcgatcgg tagcgaagca gcgaccgggc gcggcgtcac cgcgctggcg
    25501 ggcagggcgg gcgcggcgag ataccggggc ggcggcttca gcgcgcccgc cgcgggcggg
    25561 tccacgctcg agagcaatgg gttggtggcc gagctcggca tctcctccag ggcgctcggc
    25621 gcccccagca cggcgctgag ctccgccgag gcgcgccgcg cggaagcgaa gcggtcggcg
    25681 ggcgagcgcg cacaggcgcg cgcgaaccag gcgtcgaagc cctccggcac cgaccccttg
    25741 cgcgagggca ccggcagcgg gcgtgtgcag atcgcgagca ccagctgcgg cagcgtgggc
    25801 tcgcagaagg gcagctggcc cagcaggcag cggtaggcga tcacgcccag cgcccagatg
    25861 tccgtgcgct ggtcgagcgt cttggcgcct tcggcttgct ccgggctcat gtacgagggc
    25921 gtaccgagca aggagcccgt gcgcgtggcg ttgccgaccg gagcttcgac cttggcgatg
    25981 ccgaaatcga agaccttgaa gatctcctcg tcatcgttgc gcacgatgaa gatgttctcc
    26041 ggcttcagat cgcgatggat gatgcccgct tcgtgcgcgc gcgtcatggc ccgcgccagg
    26101 tgctggatca cgcgcgccgt ctcggcggcg ctcaggcgac ccacgcgctc caggcggtca
    26161 gcgagcgact cgccctccag cagctccatg gcgatgtagg gcgtcgtgcc gtcgatgcct
    26221 tgatcgagga tctgcaccac atgcgggctg cgcagagcag ccgccaggcg cgcctcgcgc
    26281 aggaagcgca gggtcgccga gctgtcgagc tgcgctggtc cctcgatcag cttgagcgcg
    26341 acggccgcgt tcagcgagag gtgctccgcc ttccagaccg agcccatccc gcccgtcgcc
    26401 aatggacgga tcaggcggta tcgaccaccc agtatctcgc ccggcttgtg ccccatgacc
    26461 gaagctaacg agaggacgcg ctcatggcaa acgtaagttg ttggtttgga gagacttttc
    26521 gctgccgttt cggcgtgctt tcagcgtgct ttcaccagcg tcgtgcggtg ggggcatgct
    26581 ggtgttgcgc gggttgctct ggctccgagc cattctctcg ccatccgtgg atcgtcgatc
    26641 ggggcttctc ttttgtccgc gcttctgaac gctgactggg ctctggcaaa atgccagcga
    26701 gaccagaacg aacggcgcac tcagcgatgg cgagggcgag cgcgctggct cgccaggtag
    26761 gtcagcttgc gcatggagtg cacgaagccc accggggtga tgccgcggcc atcgcggaat
    26821 gggtcgaagc gcagcgcctc ttgatcgagc tccaccggct cgcgcagatg gagttgaacc
    26881 acgctgcgaa agctttcgcc gacgggtgga aaaagcggcc ccgagtacgc ggcgagctcg
    26941 aggcgcaagc tcgcctcgcc ggctcgcacc gcgcgctcca gtcgttcgct gcgggtcgga
    27001 cccggcggcg cctcacgctc cggggcaagg cgccattcga cgcgccgggt gccgacctgg
    27061 aacggcgaga cgccgtagta gtcgttggcg agaaagtcgt ggaagtcagt ggtccagggt
    27121 gcaaacgcca tgctccacgg cctgcggatg gtggcgagca acaaatcctg atcgccgggc
    27181 tgcggagcgg catggagcgg gtcggagcca aagcgaatgg cgcaaccgag cacgtcggtg
    27241 cgttcaccgc gcttccacca ggcggaagac cagcggacca gggcaggccc agccagggcc
    27301 gaagccaggc cggcgagcgc ggagctggcc ggcgccgcct ccacctcggc ccggtacaac
    27361 acaccgcggg ggtggaacat gcggctgcgg cgcacagcgc tcacgctgcc ggtgagcggc
    27421 gcgagagcga gccccgcgag ccagccggtg accgtctttc cgggcatacc tgtagcgcta
    27481 gcccgcttcg caggtcacgc ccgagccaac gcagcggctg ctgcgccttg cccctcttcg
    27541 ctcggcagcc ccgctccggt gcgctggccc cgtgcagagt gtgtcgagcg tgtagagatc
    27601 gtagcgatcc gcacgagtgc gctgcttccc ggcgagcgct cattgccgaa gcgacggagc
    27661 agcgcgaagc gagaagagtt tgtccttgct cattggaagg accgcgacta ctcagagcgc
    27721 ccttcgagca cgttcgcatc attgcaggct gatggatgat ttgcgctctc gtggatcacg
    27781 agcagaagcg cagcagacgt catgcgcaga tcgatcgctg ctgaggcgtt ttgacgctgc
    27841 gctagcgctc gtccgtcgtg agacgcgagc agcgacgcag cgcgaaagtt tgttgcgcag
    27901 cgcgtgaact agcccttgcg catgcctgct gcgcgccggc agactcgctg cgtaatggct
    27961 gggcggaggg caaccaatcc cgaaacgctg cgacagcgcc ccacgacatc ggcgggcgcg
    28021 tcagctcctg gagcgcaggc ggcagtaaca cagcgcagca ctcgcgcttt ctacctgcgg
    28081 gcgagcacgt tcggcggcta tcgcgtggag cgtatcctga gcaccggcag cctgggcgcg
    28141 gtggtcagcg cgcggcacct cgttcgcgac gaaatcgtgg tgatcaagct gttgcatccg
    28201 gatctgtgcc acgatccggt gtcggtcgcg cgcatgtcgc gcgaggcgca cgtgctcgcc
    28261 cacatcgcca gcgaaggcgt ggtgcgcctg ctcgacacgg gctggaccga tgaagtgggc
    28321 ccctacgtcg tgctcgagca cctcgtgggc atggacctgg cccgcctgct ggagaccagc
    28381 gggcccttgc cgctggcgct ggccgtcgac tacctgctcc aggcctgcga agcgctcgcg
    28441 gtcgcgcacg cctcgggcat cacccatcgc gatctgaaac ccgagaacct gttcgccgtg
    28501 cgcggcttct gcggggacac cccgcacccc gagggggctg ccccgctgct caagctgctc
    28561 gatttcggca tcgcccagca cggccccgag agcttcccct atcgcgagat cgcgctggaa
    28621 gacgcggagg cgccgctgct cggcacgccc gcgtacatgt ctccggagcg cattcaggac
    28681 atgcccaccg atcagcgctc ggacatctgg tcactgggcg tcgtgctgca cgagctgatc
    28741 accggccgcg tcccgttcag ccgccatgat cgctccaacc tccagcgcgg cgaagagtcg
    28801 gacatctgcg atcgcatcgt cagccactcc ttcgagctgg agaacaaccg ctcgctcctg
    28861 ccgtcgcccc tgcgcgcggt gattcaccgc tgcctgcagc gcaaccccgc ccatcgctac
    28921 cagagcgtcg aagagctggc cgcggcgctg cgccgcacca tcgctttgcc cgagcgctgg
    28981 cgcggcacgc agaccggcaa gttcagccgg cagctgctgg cacaggagct ggcccaggcc
    29041 gaagcggcgg ctcctcccgc ggcgccctcg cgctggagct cggccgcgca ggaaatcggc
    29101 gcggccttgc aacgactgcc cgcgcgcctc gcgccgctgg cggcagcgct gcgcggccgc
    29161 gccccgcgcc cgcacgccct ccgtggctgg gtgcgcgcgc actggggctt cggcgcctgg
    29221 ctcgcgacgg cgctcaccct ggccgcgctg gccggcggct tgctggcaaa gctggagcag
    29281 agccgcgccg ccgtcgcgcc atcggcgctc tcgcaatagc ttcaggggct cgcctccgcc
    29341 ggtccgcgtg cggccccagt atctgcccac cgtacgtctc tcggcgcgtc cgcgcgagcg
    29401 cgcctgctcg ctacttccgg ctcagccagc gcgcatcttc ctttggcgtc tggcggtcga
    29461 agtccaccac gtacgcgagc aaggtctgcg tggctcgcgc ccagcgctcc gcgcggccca
    29521 gctcgctcgc cgtcagcgcg atcggctcgc gcacttgcgc gaggtcgtcg ggagcgtagg
    29581 cgatgggtgt gccctgggcg gggcggaaga gtgagaactg tggggtctcg caggcatagt
    29641 gccagccgcg caagatcagc gcgctggcat tcgggctctg ggggtccagc aaggagtggc
    29701 ccatgtagcc gttgtcgtcg tggatgcccg cgagctcgct gatcgtcggg gcgaggtcga
    29761 cgtggctggc gatgcgccgc tgagcgccgc gcggcaggcg ctcgtcggag ccgtgcacga
    29821 tcagcggcac ccaggtggtc tcgtggcgca ggttgtcgtg tccgagcact tcgccacgct
    29881 cgccgagatc gaaggcgtgg tcgccggtga tgatccacac ggtgcggccg aaccagggct
    29941 catcgcgcag gctctcgtag agctcgcgca cgacgtcgtc cgtgtagtgc atggtgttgc
    30001 gcaagcgcgc tcgagcggcg ggcagctgcg ctgccgatgg cagatcgagc gcc
//
LOCUS       DASTKU010000617         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1235932, whole genome shotgun sequence.
ACCESSION   DASTKU010000617 DASTKU010000000
VERSION     DASTKU010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235932"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..>2172)
                     /locus_tag="VFQ61_21675"
     CDS             complement(302..>2172)
                     /locus_tag="VFQ61_21675"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HET9957128.1"
                     /translation="WSYVEEELKPEQRKVRQSLEALHAVGQQTSDALQPVYKQIQSLT
                     STGGQVHAVLSGMIRAPDATRKLVELCGIMALPLIALVLLRTYGPQLPTLSTAIVGGI
                     ASSTAALARGVSWVRDRAQQTMEHLEPLIGFRDRVAAALEAAEAKKAAEVARNEREVA
                     AKSAALEVAKREVRESESRVQSAIEALAAAVNGAGIARFIEQRLASSDYQKHLGLIAL
                     VRRDFEQLTQLIQGHNAQRLHLQEDAANIREALRRARPNLAEDELRASFENLGVNRVI
                     IYIDDLDRCPSERVVEVLQAIHLLLAFPVFTVVVGVDARWMAHALSKEFPALLGTAAE
                     KAGVEGNGRVSPSDYLEKIFQIPFWVPPLDELTTCALLDKLAGGRKNEATLAGYSPDD
                     LFGEEGASFGSVQAGLGSGGDSHNSGTYPKLSESLSERSRSTQPGANGSEGDSPHPGL
                     IRPVALALDKTEVQAMRELALVIGRSPRATKRFVNSYRLLKAALTDKERGELGLEPKS
                     TSLNFEKLRTPMLLLAIVTGAPSLAARFLAANPPASASLQDKAPSPLEYLAELARQAT
                     SSVEVADTFARVRQFCGGPQRTRWADIEYGEIARWATRVSQFSFEDLASPLAESVA"
BASE COUNT          396 a          720 c          663 g          393 t
ORIGIN      
        1 ccggagtaat ggcagcgatt cacgaaaaag atcgtgaaaa cgtacgccgc atgagcttcc
       61 ccagctccgt cgaaggcgcg aaccgccccg ttgcgggaca gcccttggtc gcaccgcaaa
      121 ttacgcgagg tcgtctggcc cgcgaacgcg gctgcgggcc gagggcgtgc ttggggccga
      181 attggccgtg gggcggcggc agggcgggcg cctcgcggtc gtctccgcgt caccgaggca
      241 tcgattcggg accgctatgt tcgtgggcta cgcctcgcgg gccttgcttg aagcgagacg
      301 ttcaagccac ggactcggcg agtggactcg ccaagtcctc gaaggaaaat tgagaaacgc
      361 gggtcgccca gcgcgcaatc tcgccgtact cgatgtccgc ccagcgtgtc cgttggggtc
      421 ccccgcaaaa ctgccgaact cgagcgaacg tatccgccac ctccacactc gaggttgcct
      481 ggcgggccag ctccgcaagg tactcgagag gtgatggagc cttgtcctgc aaagacgcgc
      541 tcgcgggtgg attggcagcc aaaaagcgcg cggccaggct cggtgccccg gtcacgatcg
      601 cgagcaacaa catcggagtt cgaagtttct cgaagttcag cgaagtgctc ttgggttcca
      661 aacccagttc gccgcgctcc ttgtcggtca acgccgcctt gagcagacga tagctattga
      721 cgaaacgctt ggtcgcgcgc ggagatcggc caatcaccag ggcgagctcc cgcatggcct
      781 gtacctcagt tttgtcgagc gcgagtgcca ccggacgaat caacccggga tgcggagagt
      841 ctccttcact gccgttcgcg ccgggctgag tcgaccgcga acgctccgag agagactcgg
      901 agagcttcgg gtacgtcccc gagttatgtg aatcgccgcc ggaccccagc cctgcctgaa
      961 cgctcccgaa cgaagcgccc tcttcaccaa acaggtcatc cggcgagtac ccggcgagcg
     1021 tggcttcgtt ctttcgccca cccgcgagtt tgtccaacag agcgcaggtc gtaagctcgt
     1081 cgagcggggg aacccaaaac ggaatctgga agatcttctc gaggtagtcg gagggagaga
     1141 cacgtccgtt gccttcgaca cctgcttttt ccgcggcggt gccgagcaac gccgggaatt
     1201 ccttgctcag cgcgtgagcc atccagcgcg cgtccactcc gacgacgacc gtgaacacgg
     1261 gaaacgcgag cagcagatgg atagcctgca gcacttccac gacacgctcc gaggggcagc
     1321 ggtccaagtc gtcgatgtag atgatgacgc gattgacgcc taggttctcg aaagatgccc
     1381 tgagctcatc ttccgctaag ttcgggcgcg cgcggcgcag cgcctcgcgg atgtttgcgg
     1441 cgtcctcttg cagatgcaag cgctgcgcgt tgtgaccctg gatcaactga gtgagctgct
     1501 cgaagtctcg acgcactaaa gcaatcaagc ccaggtgctt ctggtagtcg ctgctcgcca
     1561 ggcgttgctc gatgaagcgc gcgatccccg ctccgttgac ggccgctgcc agcgcctcga
     1621 ttgccgattg cacgcgtgat tcgctctcgc gcacctcgcg cttggccacc tccaaggcgg
     1681 cactcttggc cgcgacctcc cgctcgttcc gcgccacctc ggccgccttc ttggcctcgg
     1741 ccgcctccaa cgcggccgcg actcggtcgc gaaagccgat cagcggctcc aggtgttcca
     1801 tcgtttgctg ggctcgatca cgcacccagc tcacgccgcg agccaacgcg gcagtgctgc
     1861 tcgcgattcc acccacgatc gcggtagata gcgtcggcag ctgcggcccg taagtccgaa
     1921 gcagaactag ggcaatcagc ggcaaagcca tgattccaca cagctctacc aacttccgag
     1981 ttgcgtccgg ggcacggatc atcccgctca gtaccgcgtg cacttggccc cccgtggacg
     2041 tcaacgactg gatttgtttg tagacgggtt gaagagcgtc actcgtctgc tgcccaaccg
     2101 cgtgcagagc ctcgaggctc tgccgaacct tgcgctgctc cggcttcagc tcttcctcga
     2161 cgtaactcca ta
//
LOCUS       DASTKU010000618         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_120077, whole genome shotgun sequence.
ACCESSION   DASTKU010000618 DASTKU010000000
VERSION     DASTKU010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120077"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFQ61_21680"
     CDS             complement(<1..528)
                     /locus_tag="VFQ61_21680"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9957129.1"
                     /translation="MSASFIPVCEPLLLGNELKYVTEAVSKGWISSSGGFVKQFEQKF
                     AEYLGSRHAVTASNGTAALHLALVAAGIGPGDEVIVPTFTMIASCAAICYTGAKPVFV
                     DCERDTFNLDVSRVREKLTPRTKAIMPVHIYGLSCDMAPLLELAREKKLTIVEDAAEA
                     IGSRYQGKPCGAIGTL"
     gene            complement(635..1042)
                     /locus_tag="VFQ61_21685"
     CDS             complement(635..1042)
                     /locus_tag="VFQ61_21685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957130.1"
                     /translation="MQGVELIREGEQLLAILVRANASSTEKYNFLTDSSAPLQLGMNF
                     YRSGDVIPGHSHLPREIKTSEIQEVILIGHGRTRLKLYDDQRKQVAETILEKGDLVLL
                     VSGGHGFDILEDTKILEVKQGPYDGKVKDKVTF"
     gene            1266..2033
                     /locus_tag="VFQ61_21690"
     CDS             1266..2033
                     /locus_tag="VFQ61_21690"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9957131.1"
                     /translation="MSVFGSYSRYYNLLYRDKDYAGEADYIRSLIEKYRPGAKTVLDL
                     GCGTGRHAQLLAGHGYELVGVDRSTEMLNVARQNASAAGGAARTEFLEGDVRSVRLGR
                     TFDVVVSLFHVMSYQNSNADLRAALATMRTHLAPGGLFIFDCWYGPAVLSLKPSTRIL
                     RLEDSESSVTRLAEPVLHATANVVDVNYHVFVKDKASAQVTELRETHSMRYLFSPEVE
                     LLAGLEGLRIDATLAFRADSPPGFDTWTAVFLGGHAS"
     gene            2062..>3062
                     /locus_tag="VFQ61_21695"
     CDS             2062..>3062
                     /locus_tag="VFQ61_21695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9957132.1"
                     /translation="MNQHSGSGVTVSVLCFPYYPSRDTGRGHDRYILELVENLQALGD
                     ELRVDVVEQGFSKGVLAAGGKVLRLAAELLSKRSDVYHAISPNGGAVAALLGKRPLVV
                     TIHDLIPFRVSGFDSSWKARFVRFCSRVSIEKSAAIVVPYRVTKDEIVNTLGGSAERI
                     FVVNYGVDHATYYPRPELTRSPNRILYIGEVSRSKGVDVTIRAFAEVKRQVPNAELVI
                     GGKSSKDQAMLEELARSLGVQDLVFKGFIPEEELATYYSTAAVMVFPSRYGFGLSTLE
                     AMACGTPVVAAAALDAQEFAEDIDILVPPDDHDALAARITRILTDTAFREELSSKGIT
                     "
BASE COUNT          577 a          938 c          948 g          599 t
ORIGIN      
        1 gagcgtgccg atcgctccgc acggcttgcc ctggtagcgt gaaccgatcg cctccgcggc
       61 atcttcgacg atggtgagct tcttctcccg cgcgagctcc agcagcggag ccatgtcgca
      121 cgacaacccg tagatgtgga cgggcatgat cgccttggtg cgaggcgtga gcttctctcg
      181 tacgcgggac acgtcgagat tgaaggtgtc ccgctcgcaa tccacgaaca ccggcttcgc
      241 tcccgtgtaa cagatggcgg cacaggacgc gatcatggtg aaggtgggga caatcacctc
      301 gtctcccggc ccgattccgg ccgcgactag ggccagatga agagcggcgg tcccgttgct
      361 ggcggtaacc gcgtgccggc tgcccaggta ctccgcgaac ttttgctcga actgcttcac
      421 gaatccgccc gaagaggaga tccaaccctt cgaaacggct tcggtgacgt acttcagttc
      481 attgccgagc agtagaggtt cgcaaaccgg gatgaaagag gcgctcaaag tgcgcgcatc
      541 ttagatcgaa aggcaggttc gcgaaaggcc ggccgaggca gccggcagtg ctcggggggc
      601 ggcgctgcct gtggagttct tcgaagcagg gtgcttagaa cgtgaccttg tctttgactt
      661 tgccgtcgta cggcccctgc ttcacttcga ggatcttcgt gtcttccagg atatcgaagc
      721 cgtggccgcc cgagaccaac aacaccagat cgcccttctc gaggatggtc tccgcgactt
      781 gcttgcgctg gtcatcgtag agcttgagcc gagtgcgccc atgcccgatt aagatgactt
      841 cttgaatctc actcgtcttg atctcacgcg gtaaatgcga gtgccccggg atgacgtcgc
      901 ccgagcgata gaagttcatt ccgagctgga gcggagcgct gctatcggtc agaaaattgt
      961 acttttcggt gctagaagcg ttggcgcgca ccaaaatcgc gagcagttgc tcgccttccc
     1021 ggattagctc tacaccttgc atggggcccg ttcacgtacc ctccgccggt ggctgaagca
     1081 aggcgaaggc gagaggagaa ggaaagggat cctttcggtc aaggggccga gtttcccaaa
     1141 agacggaccg ggttcccgaa cggaagcagc cggaaggctg cgcccgcccg gtcgcgcaag
     1201 aggctgttgt cttgccaagg tagaggaagg ctggcagaaa ctgcagcgcc ttcggacgag
     1261 tgcccatgtc agtcttcggc tcctattccc gttattacaa cctgctttat cgcgacaagg
     1321 actacgccgg agaggctgat tacattcggt cattgatcga gaagtatcgt cctggcgcaa
     1381 agacggtcct ggacctcggc tgcggaacgg ggcgccacgc gcagctcctg gcggggcacg
     1441 gctacgagct cgtgggggtc gatcgctcca ccgaaatgct gaacgtcgcc cgccagaatg
     1501 cgtccgccgc cggcggcgct gctcgcaccg agtttctcga aggggacgtg cgatcggtac
     1561 ggcttgggag aacgttcgac gtcgtggttt cgctcttcca cgtgatgagt taccagaaca
     1621 gcaacgcgga tctgcgcgcg gcgctggcga cgatgcgcac ccacttggct ccggggggcc
     1681 tgttcatctt cgactgttgg tatgggccag ccgtgttgtc gctcaagccc agtactcgaa
     1741 ttctgcgcct cgaagactcc gaaagcagcg tgacgcgctt ggcggaaccg gtgctacacg
     1801 cgaccgcgaa cgtggtggac gtgaactacc acgtgttcgt caaggacaag gcctctgcgc
     1861 aagtgaccga gctgcgcgaa acccactcca tgcgctatct cttctctccc gaggtggagc
     1921 tgctggcagg cttggagggg ctccgcattg atgccacgct cgcattccgc gccgatagtc
     1981 ctcccggatt cgatacttgg accgcagtct ttttgggcgg gcacgccagt tgagccctag
     2041 ttcgcaacgg agaatcagca catgaatcag cattccggct cgggcgtcac ggtgagcgtc
     2101 ctgtgcttcc cgtactatcc gtcgcgcgac acgggtcgtg gccacgaccg gtatatcctg
     2161 gagctcgtcg agaacctaca agcgttaggc gacgagctgc gcgtggatgt ggtcgagcaa
     2221 ggcttctcga agggtgtact cgccgccggc ggcaaagtgc tgcggctagc cgcggagttg
     2281 ctgagcaagc gctccgatgt ctatcacgcg atctccccga acggcggcgc cgtcgcggcg
     2341 ctgctcggta aacgcccgct ggtcgtgacc attcacgact tgatcccgtt ccgcgtgtcg
     2401 ggcttcgact cctcgtggaa ggcccgattc gtgcgcttct gcagtcgagt gtcgatcgaa
     2461 aagagcgctg cgatcgtggt gccgtatcgg gtgaccaaag acgagatcgt gaacacgcta
     2521 ggcggctccg ccgagcgcat cttcgtcgtg aactacggtg tagaccacgc cacgtactat
     2581 ccacggcccg agctaacacg ctctcccaac cgcattctgt acatcggcga ggtcagccgc
     2641 tccaaggggg tggacgtcac gattcgcgcg tttgccgagg tcaaacgcca agtccccaat
     2701 gcggagctcg tgatcggcgg aaagtccagc aaagatcaag cgatgctcga agagctggcc
     2761 cggtctctcg gtgtgcaaga cctcgtattc aagggtttca ttcccgaaga agagctcgcg
     2821 acctactact ccacggccgc cgtcatggtc ttcccgtcgc gttacggctt cggcctatcg
     2881 actctggagg cgatggcctg cggcactccc gtagtcgccg cggcggcgct agacgcgcag
     2941 gaattcgccg aagacatcga tatcctcgtc ccgcccgacg accacgacgc tctcgccgcc
     3001 cgcatcactc ggatcctgac cgatacggcg tttcgcgaag agctatcgag caaaggcatc
     3061 ac
//
LOCUS       DASTKU010000619         4204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_566243, whole genome shotgun sequence.
ACCESSION   DASTKU010000619 DASTKU010000000
VERSION     DASTKU010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566243"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..882
                     /locus_tag="VFQ61_21700"
     CDS             256..882
                     /locus_tag="VFQ61_21700"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HET9957133.1"
                     /translation="MSKALSASNAVQPLPFKAGTLNGLSERMIVSHHENNYGGAVKNL
                     NRVEQELSQIAADTPPFVVAGLRQSELTFRNSKTLHEAYFGNLGGNGKRSGMIEKALT
                     EAYGSSARWEEHFRATGAGLGGGSGWVMLDLELSTGELRTAWSGHHTQTLATAIPLLV
                     LDMYEHAYQMDFGAAAARYIDAFFANLNWDEVNRRLERALAASRALRA"
     gene            complement(918..2105)
                     /locus_tag="VFQ61_21705"
     CDS             complement(918..2105)
                     /locus_tag="VFQ61_21705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HET9957134.1"
                     /translation="MTQIQEKPTTESESALVPCSLGEFVRYFLYLGSLGFGGPIALAG
                     YMQRDLVERRRWLSKTDYVEGLALAQLAPGPLAAQLAIYLGWVRAGVSGATLVGLAFV
                     LPSFLMVLVLASLYVTFGGMPWMRGVFYGIGAAVIAIIARSVVKLGKLTLAKDPLLWA
                     VFLASAVVTAWTESEIIWLFIASGVITLFIKAPPKRVTSSVAGFAPVWLLAGLHGAAT
                     PNILATIAWYFAEAGAFVFGSGLAIVPFLHGGVVTRFHWLNDQQFLDAVAVAMITPGP
                     VVITVAFIGFLVAGLAGACVAALGTFVPCYLFTVLPAPYFRRFAKNAQIKAFVDGVSA
                     AATGAIAGAAFVLGRRAIFDIPTALICLVTLALVTKFKKIQEPLVILVSGAVGLAISS
                     FRP"
     gene            complement(2102..3133)
                     /locus_tag="VFQ61_21710"
     CDS             complement(2102..3133)
                     /locus_tag="VFQ61_21710"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HET9957135.1"
                     /translation="MSPVPVMLEANRWLLLIHQLPKEPAYLRVKIGRRLSRIGALALK
                     NSVYVLPKTESASEDFQWVRVEILEGGGDATVLEARFVEGLTDEAVEGQFREAKDVEY
                     AELLREARALPIEPVGKRKRALSEDERAALLADITRLEQRVEELATTDFFGASGRVAV
                     TGVLRELRTRVEPPVAPSPPDAPPSFRARVWVTRTGIHVDRIASAWLIRRFIDPDAVF
                     KFVPARGYEPAPGELRFDMFDAEFSHEGDLCTFEVLCQRLSLEQPGLRAISEIVHDID
                     LKDGKFARPETQGVATQITGLALRHRDDEARLQHGSELFEQLLAYFSRKKPEAPAARV
                     PETSRKKRP"
     gene            3374..3742
                     /locus_tag="VFQ61_21715"
     CDS             3374..3742
                     /locus_tag="VFQ61_21715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9957136.1"
                     /translation="MRLNHIDLQVPDVQETAVFFERWFAFEHVSNRHSPSVAILRGDG
                     DFTLVLQRRADDERYPEGFHVGFLLDDVREVLSFHARAREAGLSISDVQHTGRGTLTY
                     LRGPGVLIEVSCRARVGERP"
     gene            3829..>4204
                     /locus_tag="VFQ61_21720"
     CDS             3829..>4204
                     /locus_tag="VFQ61_21720"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HET9957137.1"
                     /translation="MTRGALLFDLDGTLTDPREGITRCLQHALETIGAAVPGERELQR
                     WIGQPLQQSLRSHLGANNEALVDKGIAAYRERFAAIGMFENVVYAEIPAALAELKASG
                     WAMFVATSKPSLFARQTVEHFGL"
BASE COUNT          843 a         1343 c         1278 g          740 t
ORIGIN      
        1 acaagttaca tcgcttattg cgttgctttc gggtgttcgt gaatcttagg atggtgtccg
       61 caatcgaggt tgcgagcgac ggaggcagaa gtgacgtctt cccaagaccg tatgctttct
      121 cggcgtgacg taatgttcgc cacgggcgga ttcgcggctg cggtgagctg tgcaacggcc
      181 cacgcccagg cgccgacgtc caccgcaaac cctcagccag tatccacgac caagaccgga
      241 gaatccgcga tgcccatgag caaggccctg agtgctagca acgccgttca gccactcccg
      301 ttcaaggccg gcacgctgaa tggcttgtcc gagcggatga tcgtctcgca tcacgagaac
      361 aactacggag gggccgtgaa gaacctgaat cgggtggagc aggagctttc acagatcgcg
      421 gcggatactc cgcccttcgt cgtggcgggc ttgcggcaga gtgagttgac tttccgcaat
      481 tcgaagactc tccacgaggc gtacttcggt aatctgggcg gcaatggcaa gcggtcgggg
      541 atgatcgaga aggctctcac ggaggcttac ggcagctctg cgcgctggga agagcacttt
      601 cgcgccaccg gcgccggctt gggtggaggc agcggctggg tcatgctcga cctcgaactc
      661 tcgacgggcg agctgcggac cgcatggtcc ggccaccaca cccagacgct agcgaccgcg
      721 atccctttgc tggtactcga catgtacgag catgcttatc aaatggactt cggcgcggcg
      781 gcggcgcgtt acatcgacgc attcttcgcg aacctcaact gggatgaagt caatcggcgc
      841 ttagaacgcg cgctcgcggc atcccgggcg ttacgcgcat agcgcggcgc gcccagcgcc
      901 gcgtgcgagg gttgcgccta tggcctgaag ctcgagatcg cgagccccac cgcaccactg
      961 acgaggatga cgagcggctc ttggatcttc ttgaacttcg tgacgagcgc caaggtcacc
     1021 aggcaaatca gcgccgtggg gatatcgaag atggcgcgcc gccccagcac gaacgccgct
     1081 cccgcgatcg cccccgtcgc ggccgcgcta accccatcga cgaaggcttt gatttgggca
     1141 ttcttggcaa agcgccggaa atatggggcg gggagcaccg tgaacaaata acaaggcacg
     1201 aacgtaccga gagcggcgac gcacgcgccc gccaaccccg cgacgagaaa cccaatgaac
     1261 gcgaccgtaa tcacgacggg gcccggcgta atcatggcca ccgccacggc atccagaaac
     1321 tgttgatcgt tcaaccaatg aaaccgcgtg accactccgc catgcaggaa aggcacgatg
     1381 gccaggccgc taccgaacac gaacgcaccc gcctccgcga agtaccaagc gatggtggcc
     1441 aagatgttcg gtgtagcggc gccatgtagc ccagcgagta gccacacggg tgcgaagccg
     1501 gcaacactcg atgtcacccg cttgggcggc gccttaatga acagcgtgat gacccccgaa
     1561 gcaatgaaca gccagatgat ttccgactcg gtccaggctg tcactaccgc actggctaga
     1621 aatacggccc agagtagcgg atctttggcc agagtgagtt tgccgagctt gacgacactg
     1681 cgcgcgatga ttgcaatcac ggccgccccg atcccgtaaa acacgccgcg catccagggc
     1741 atgcccccga acgtcacgta taaactcgcg agcacgagca ccatcaaaaa ggagggcaga
     1801 acgaaagcca gcccaaccag cgtggcccca gacacccccg ctctgaccca acccaggtaa
     1861 atcgcgagct gtgcagcgag cggacctggg gcgagttggg ccagggccag cccttccaca
     1921 tagtctgtct tggagagcca gcgccgccgc tcgaccaagt cccgttgcat gtagccggcg
     1981 agcgcgatgg ggccgccaaa gccgagcgat cctaaatata gaaagtaacg aacgaactcc
     2041 cccaacgagc acggaacaag cgctgattca ctctcagtcg tcggtttctc ttgaatctgt
     2101 gtcatgggcg tttcttccgt gaggtttcag gaacccgggc tgcaggcgcc tccggtttct
     2161 tgcgcgagaa gtacgcgagc aattgttcga acagctcact gccgtgctgg aggcgcgctt
     2221 cgtcatcgcg gtgccgcagc gctagccctg ttatttgagt agctactccc tgagtctctg
     2281 ggcgcgcgaa cttgccgtct ttgagatcga tatcgtgcac gatctcggag atcgcccgca
     2341 ggccaggctg ttcgagactt agtcgctggc acagcacctc gaaggtgcaa aggtcgcctt
     2401 cgtgggagaa ctcggcgtcg aacatatcga aacgcaactc cccgggtgcc ggctcgtaac
     2461 ccctcgccgg cacgaacttg aacaccgcgt cagggtcaat gaatcgacga atgagccaag
     2521 cgctcgcgat gcgatccacg tgaatgcccg tgcgagtcac ccacactcga gcccgaaacg
     2581 agggtggcgc atccggaggc gacggcgcga ccggcggctc gactcgtgta cggagctccc
     2641 gcaaaactcc ggtaaccgcc acgcgcccgg aagccccgaa gaaatcggta gtggccaatt
     2701 cctccacgcg ctgctcgagc cgcgtgatgt ccgcgagcag cgcggctctc tcgtcctcgc
     2761 tcagcgcgcg cttacgcttc cccaccggct cgatcggaag cgcgcgcgct tcgcgcagaa
     2821 gctccgcgta ctcgacgtcc ttggcttcac gaaactgccc ctctacggcc tcgtccgtca
     2881 acccctccac gaaccgcgct tcgagcaccg tcgcatcccc gccgccctcc agaatttcca
     2941 cgcggaccca ctgaaaatcc tcgctggcgc tctccgtttt gggcagcacg tagaccgagt
     3001 tcttcagcgc gagcgcgcca atccgggaca gccgacggcc aatcttcacg cgcaagtaag
     3061 ccggctcctt ggggagctga tgaatcagaa ggagccagcg gttggcttcg agcatcacag
     3121 gcacggggct catgctacag gtgaatcatc ggtaggccaa gcaacgattc agctgatcgt
     3181 ttcgtttcgg cgtcctcctc cccagcgccc ttgactatcc caaggctctc gcgcatacct
     3241 gtgcgcagtc ggaaaacggc agacgggtcc ggtgagcccc cgtcagcgcg cagatggtct
     3301 gtgtgcctct tgtcccgtcc caagacgtgt gaccctcgac ctagggcacg ggtgtgcccg
     3361 aaaggttcgt gtcatgaggc tcaatcacat cgatctgcag gttccggacg ttcaggagac
     3421 tgcggtcttt ttcgagcgct ggttcgcgtt cgaacacgtg agcaatcgcc actcgcccag
     3481 cgtcgcaatc ctgcgcggcg acggcgactt caccctcgta ctccagcgcc gtgccgacga
     3541 cgaacgttat ccagaaggct ttcacgtcgg ttttctgctg gacgacgttc gagaagtgct
     3601 gagtttccac gcgcgagccc gcgaggcggg cctctcgatc agcgacgtcc aacacaccgg
     3661 ccgcggcacg ctgacgtacc tgcgcgggcc cggagtgctg atcgaagtga gttgtcgggc
     3721 acgggtgggc gagcgtcctt aggcacgtct cattgatgat ctgggggcgc accgaggcgg
     3781 gcgcgacaac ccttgtcgtg accgctcggt gtggtgataa agagcgacat gactcgcggc
     3841 gctctgctct tcgacttgga cggcacgctg actgatccgc gggagggcat cacgcgctgc
     3901 ctccagcacg cgctcgaaac gatcggagcg gcggttcccg gggaacgcga gctacagcgg
     3961 tggattggac agccgcttca gcagtcgctg cgtagccatc tcggcgccaa taacgaggct
     4021 ttggtcgaca aaggcatcgc tgcctatcga gagcgctttg cggcgattgg catgttcgag
     4081 aacgtcgtct atgccgagat tcctgcggcc ctggcggagc tgaaggcgag cggctgggcc
     4141 atgttcgtgg cgacgtcgaa gccaagcctg tttgctagac aaaccgtcga acattttggg
     4201 ctct
//
LOCUS       DASTKU010000620         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_788950, whole genome shotgun sequence.
ACCESSION   DASTKU010000620 DASTKU010000000
VERSION     DASTKU010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788950"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFQ61_21725"
     CDS             complement(<1..205)
                     /locus_tag="VFQ61_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957138.1"
                     /translation="MRASFVESRTGRHYTRNYDRFVADATTPLIAVVDSECDDGMSGD
                     VVANALKRSGAGLARHLDAPAERV"
     gene            complement(708..1091)
                     /locus_tag="VFQ61_21730"
     CDS             complement(708..1091)
                     /locus_tag="VFQ61_21730"
                     /inference="COORDINATES: protein motif:HMM:NF014892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRAM domain-containing protein"
                     /protein_id="HET9957139.1"
                     /translation="MKTALLPGEILIKDGAANLQRGIETVGGRLYLTNERLVFEPHGF
                     NVQTDVVAIALVGVTGARKCWTKLLNLIPLVPNSVAVATAEGREHRFVTWGGQAWIAA
                     IQAQKLNERCVGRATESQDLPNDQG"
     gene            complement(1154..1666)
                     /locus_tag="VFQ61_21735"
     CDS             complement(1154..1666)
                     /locus_tag="VFQ61_21735"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9957140.1"
                     /translation="MISRRLHLVDGNLELLAAAVAGGDALEALLGVAVADGWAGFPEA
                     LPVLCASYEENPEARRWGSLFLVEPSLGVLVGLGGFKGPPSADGVVEIGYAIAPAFRG
                     QGLASDAVAQMVQRAFEDTVVRAVDAHTLGHANPSTRVLEKSGFRKIAEVNDPDEGVV
                     WHWRHERLAP"
     gene            complement(1870..2421)
                     /locus_tag="VFQ61_21740"
     CDS             complement(1870..2421)
                     /locus_tag="VFQ61_21740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957141.1"
                     /translation="MDSTDLQAFEQRARRKYEWARVRRALLGFSPAVVVITAAALTGK
                     HPMSTAALGVALFAFGVLLLWYGRDVRRAVLPGLALGALPLVLALCANHVHSCHDGVC
                     VTWCVPACALGGLGAGIGVGIIGQRARHSLGYWLGASALTLLTGAMGCNCAGYGGVVG
                     LAAGFVIGVGPAFLRTFLQRRAR"
     gene            complement(2408..>2666)
                     /locus_tag="VFQ61_21745"
     CDS             complement(2408..>2666)
                     /locus_tag="VFQ61_21745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9957142.1"
                     /translation="GRRRELSAELAPELGSELSDDDFAQRELVQRAVQAMGELSVVDR
                     ETLLATFFDEAASVSGPTLRKRRERAITRLRDAMRRLYGLD"
BASE COUNT          513 a          885 c          795 g          473 t
ORIGIN      
        1 ttactcgctc ggccggcgcg tccaagtgac gagcaagacc ggcgccagac cttttcaatg
       61 cgttcgctac gacatcgccg gacatcccgt cgtcgcactc gctgtccacg acggcaatga
      121 gaggcgtcgt cgcatcggcg acgaagcgat cgtagtttcg cgtgtagtgg cgaccagtcc
      181 tgctttctac gaaagatgct cgcattgcct cagcttcggg ccaatcgtgc gcatagtact
      241 tcagtcctct gcgctgccca acccgcttct tgtcctgctc cgtcccccaa gcgcgcccct
      301 gtggtaaggt cgcgcgccaa gttgcgtagt ctcgaccaaa agtttttcat ctcgtcactg
      361 gcgttacttg ggctgagcgt cccctgcacc ggctgcgctt ctggtgcgca gctcgcgagg
      421 gagcgcgcca tgcgcgagta tgattgtgca gtatcggaag atcatgtccg ttggctctca
      481 tcgggtccgc gtggctacga aatctaccga gtgaaagctt gcgggagatt cgtaacatac
      541 gcttgcaacg aagagtccga agagtgcatc aaagaaagtg acgatcgccc tgcaccgcgc
      601 gccccgcagg acgattgatc gcgcgtcggt cgatgcgcga tcaaaaccat cgaaccgctc
      661 cagccccagt aaccgcgttg acctggtcga tcttcgtgac gttgtcgtca tccctggtca
      721 ttgggaaggt cctgcgattc tgtggctcgc ccaacgcacc gctcgttgag cttttgcgct
      781 tggattgcgg cgatccacgc ttgcccgccc cacgtcacga agcgatgttc ccggccctcc
      841 gcagtcgcca cggccaccga gttcggaacg agcgggatta ggttcagcaa cttggtccag
      901 cactttcgag cgccagtcac ccctaccagc gcgatcgcga cgacgtcggt ttggacgttg
      961 aagccatgcg gctcgaacac gagccgctcg ttggtcagat agagccgacc accgaccgtt
     1021 tcgatcccac gctgcagatt cgctgcgccg tctttgatga gaatctcgcc gggtagaagg
     1081 gccgtcttca tggttgtctc cgttcgagaa cgatcgcact ttcgtgctca tggacgaagg
     1141 ctctccctat ctactagggc gcaagccgct cgtggcgcca gtgccaaacc acgccttcgt
     1201 cagggtcgtt cacctctgca atcttccgaa atcctgactt ttcgaggaca cgcgtcgatg
     1261 ggttggcgtg cccgagtgtg tgagcatcga ccgcgcgaac gaccgtatcc tcgaacgccc
     1321 gttgaaccat ctgcgctacg gcgtcggagg ccaatccctg ccctcgaaat gcaggagcga
     1381 tcgcgtatcc gatctcgacg acgccgtccg cggaaggcgg tcctttgaat cccccgaggc
     1441 ccacgagcac gcctagactt ggttcaacca agaacaacga accccaccta cgcgcctcgg
     1501 ggttctcctc gtatgacgcg catagaacgg gaagcgcctc tggaaagccg gcccaaccgt
     1561 cggcgacggc gacgccgagc aaagcctcga gcgcatcccc tccagcgacc gccgcggcca
     1621 gtagctcgag gtttccgtcc actagatgca agcgccgaga gatcattggg ttcgacggtt
     1681 gagcacgtcc cgccaaggtg gtcgggtcga ccggagcgtg ccatcagacc accgacagga
     1741 gatgtcggca agaccgctgc cgcgtagacc accgaccgtt gtggtccacc acgggtacga
     1801 gtttgggcgc ccctccgtga tctccgcctt cgaactccat acgagtcgag gcgcggtctc
     1861 tgaaccggct caccgagctc gacgctgcaa gaaggttcgc aaaaaggccg ggccaacgcc
     1921 gatcacgaag ccggcggcga gcccgacgac tccgccgtat cccgcgcagt tgcaacccat
     1981 cgcgccggtg aggagtgtca gcgctgaagc gccgagccaa taaccgaggc tgtgccgcgc
     2041 gcgttggcca atgatgccga cgccgatgcc ggcaccgagc ccgccgagag cgcaagccgg
     2101 gacacaccaa gtgacgcaaa ctccgtcgtg gcagctgtga acatgattgg cgcacagcgc
     2161 gaggaccaag ggcaacgcgc caagggccag gccaggcaat accgcgcgcc gcacgtcacg
     2221 gccgtaccaa agtagcaaga cgccgaacgc gaatagcgca acgcccagcg ccgcggtcga
     2281 cattgggtgc tttccagtca aggccgccgc cgtaatgacg accacggccg gactgaagcc
     2341 gagcagagca cgtcgcactc gcgcccattc gtatttgcgg cgagcgcgct gctcgaacgc
     2401 ttggaggtca gtcgagtcca tagagcctcc tcatggcatc gcgtaaccta gtgatggcgc
     2461 gctcgcggcg ctttctcaga gtggggccgc tcacggaagc agcctcgtcg aagaaggtcg
     2521 ccagcaacgt ctctcggtcg acgacggaga gctcgcccat ggcctgcacg gcgcgctgaa
     2581 cgagctcgcg ctgggcgaaa tcgtcgtccg atagctcgga gccaagctcg ggagcgagct
     2641 ctgccgaaag ctccctgcgc cgcccc
//
LOCUS       DASTKU010000621         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_788964, whole genome shotgun sequence.
ACCESSION   DASTKU010000621 DASTKU010000000
VERSION     DASTKU010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788964"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VFQ61_21750"
     CDS             complement(<1..1040)
                     /locus_tag="VFQ61_21750"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET9957143.1"
                     /translation="MSSKGRAERYELDKLRNIGVMAHIDAGKTTLTERILFYTGVSYK
                     LGEVDEGTATMDWMEQEQERGITITAAATTCYWRSHQINLIDTPGHVDFTVEVERCLR
                     VLDGAIAVFSAVEGVEPQSETVWRQADKYGVARVAFINKCDRSGADPEACVRQMSTKL
                     GCRPLVLQLAVGLESEFRGVVDLIARRLLEWSEDDEGVRVCASDVPAALTDAVDAARA
                     QLLEQLAEVDDPMLEAFANGVEPSEREIHDSIRRVTLARRGVPVLLGAAFRNKGVQPL
                     LDAVVDYLPAPVDLPPCQATDLHGRPVTRAPDPAGPLAALAFKVVHDPHAGQLTYVRV
                     YSGTLRTGESVL"
     gene            complement(1037..1450)
                     /locus_tag="VFQ61_21755"
     CDS             complement(1037..1450)
                     /locus_tag="VFQ61_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957144.1"
                     /translation="MCEAPYPSHVVRLESSVFGPSAQVICVPVARIARLLEGRGERAL
                     RRLLSPGERQDCASLGKTAQRCASRVAAKLAVRAGLPAVRRLRWCEIEVRRLESGAPV
                     LRAYDPETRAWEPLAVELSMSHTEKWAAAFVIGAA"
     gene            complement(1451..1858)
                     /locus_tag="VFQ61_21760"
     CDS             complement(1451..1858)
                     /locus_tag="VFQ61_21760"
                     /inference="COORDINATES: protein motif:HMM:NF022654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HET9957145.1"
                     /translation="MQFSVFFENPFWVGVFERVDERGYSVSRVVFGSDPRDREVWELL
                     LANLRQVRFSDPIPEPPKSMEVQKNPKRVQREAREALERAPLSTKAQDALRLALEQKK
                     KVKKEQSKAAREAERDRLFTLKQEKRKEKKRGH"
     gene            complement(1861..>2657)
                     /locus_tag="VFQ61_21765"
     CDS             complement(1861..>2657)
                     /locus_tag="VFQ61_21765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9957146.1"
                     /translation="LATLGLLTVGLFAWVEHRASDPIIPGYFIREKTLAVLGNLVTGA
                     VMYSFIFFLPLFLRAVLRASPGTGLIPLTFSLVAGSILGGRLITRYGTRTTSMLAAGV
                     LVFGALAATQLKASTPLFALAIIGPCIGIGVGGLLVSMVITMQAGTPAAYMGVSSSLS
                     QFMRNLGGLLSVNLLSAMQAAVFRSGLPLELRSTLTQAGGAKGLAESLFSGAAEGGAQ
                     VIAAYTRASTTVFWACALLGGVALLISFRLPRVRLESEAHQEAEAH"
BASE COUNT          504 a          907 c          783 g          463 t
ORIGIN      
        1 ttcaatacgg actcgcccgt gcgcaacgtc cccgagtaga cgcgcacgta cgtgagctgc
       61 cctgcgtgcg ggtcgtgcac gaccttgaac gcgagcgcgg cgaggggccc cgctgggtcc
      121 ggtgctctcg tcacggggcg cccatgaaga tccgtggctt ggcacggcgg caaatcgacg
      181 ggagcgggta aataatccac caccgcgtcg agcaacggct gcaccccttt gttgcgaaac
      241 gccgcgccga gtaggaccgg cacgccacgc cgagccaagg tcacccgccg aatcgaatcg
      301 tgaatttctc gctcggacgg ctccaccccg ttggcgaagg cttcgagcat cggatcgtcc
      361 acctccgcga gctgctcgag cagctgagcg cgggctgcgt ccaccgcgtc ggtcagcgcg
      421 gcgggaacgt cgctggcgca cacgcgcacg ccttcgtcgt cctcgctcca ctccagcaac
      481 cgccgtgcga tcaggtccac cacaccgcgg aactccgact cgagcccgac cgcgagctgg
      541 agaacgagcg gacggcagcc gagcttggtc gacatctgtc gaacgcatgc ctcgggatcc
      601 gccccgctgc gatcgcactt attgatgaag gcgacgcgcg caactccata tttgtcggct
      661 tggcgccaga ctgtctccga ttgcggctcg accccctcca ctgcggaaaa caccgcgatc
      721 gccccgtcga gcacccgtaa gcagcgctcg acctccaccg taaagtcgac gtggccgggg
      781 gtatctatca agttgatttg gtgcgaccgc caatagcagg tcgtggccgc ggcggtgatc
      841 gtaatcccgc gctcctgctc ctgctccatc cagtccatgg ttgcagtgcc ttcatcgacc
      901 tcacccagtt tgtaagacac gccagtgtag aagaggatgc gctcagtgag cgtcgtcttc
      961 cccgcgtcga tgtgggccat gacgcctatg ttgcggagct tgtcgagctc gtaacgctcc
     1021 gcacggcctt tggaactcat gctgcaccta tcacgaacgc ggccgcccac ttctccgtgt
     1081 ggctcatgga cagctctacc gcgagcggct cccaggctcg tgtttcggga tcataggccc
     1141 gaagcaccgg cgcgccgctc tccaatcttc gaacctcgat ctcgcaccac ctcagccgtc
     1201 gaaccgcggg taacccggcg cgcacggcca gctttgccgc gacacgactg gcacagcgct
     1261 gagcggtctt gcccagcgag gcgcaatcct ggcgttcccc cggcgagagt agccgccgca
     1321 gcgctcgctc accgcgtccc tccagcaacc gcgcgatacg cgctacggga acacagatca
     1381 cctgcgcgct agggccaaac acactggatt ccaagcgtac gacgtgggac gggtacggcg
     1441 cttcacacat tcagtgccct cgcttcttct cttttcgctt ctcttgcttg agcgtaaaga
     1501 ggcggtcgcg ctcggcttct cgagccgcct tggactgctc ttttttgacc tttttcttct
     1561 gctcgagcgc caaccgcagc gcatcttgag ccttggtcga gagcggtgct cgctccagcg
     1621 cctcgcgcgc ttcgcgctgc acacgcttcg ggttcttctg gacctccatg ctcttcggcg
     1681 gctccggaat cggatcggag aagcgcactt gacgaaggtt tgcgagcagc agctcccaga
     1741 cctcgcgatc gcgcggatcc gagccaaata ccacgcgact aacgctgtag ccgcgctcgt
     1801 ctacgcgctc gaaaacaccg acccagaacg gattttcgaa aaaaacgctg aattgcatgg
     1861 ctagtgtgct tctgcttcct ggtgtgcctc tgattctagg cgcactcgag ggagcctgaa
     1921 cgagatcagt agcgcgaccc cgccgagcag ggcgcaagcc cagaagacgg tcgtgctcgc
     1981 gcgcgtgtag gccgcgatca cttgggcacc accctcggcc gcgcccgaga ataggctctc
     2041 ggcgagcccc ttcgcccccc ccgcctgggt caacgtcgag cgcagctcca acggaagccc
     2101 actacgaaat acggccgcct gcatagcgga tagcaaattg acactcaaga ggccgccgag
     2161 gttacgcatg aactgcgaca gagaactcga gactcccatg tacgcggcgg gagtgccggc
     2221 ttgcatcgtg atcaccatcg agaccagcaa tcctcccacc ccgattccga tacacggccc
     2281 gatgatggcg agtgcgaaca gcggtgtcga cgctttcaat tgcgtggccg ctagagcgcc
     2341 aaagaccaac acccccgcgg ccagcatgct cgtcgtccgc gttccgtagc gggtgatgag
     2401 tcggccgccg aggatcgatc cggcaaccaa ggagaacgtg agaggaatga gtccggttcc
     2461 cggcgatgcc cgcaagaccg cgcgaaggaa caacggtaag aagaagatga acgaatacat
     2521 cacggcgccc gtaaccaagt ttccgagcac cgcgagtgtc ttctctcgaa tgaagtaccc
     2581 tgggatgatg ggatcggagg cgcgatgctc cacccacgcg aacaagccca cggtcaggag
     2641 cccgagcgtc gccaggt
//
LOCUS       DASTKU010000622        14640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_343265, whole genome shotgun sequence.
ACCESSION   DASTKU010000622 DASTKU010000000
VERSION     DASTKU010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14640
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343265"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFQ61_21770"
     CDS             <1..198
                     /locus_tag="VFQ61_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9957147.1"
                     /translation="DEAIERSLSQAEESSITQGDLLSALGEVKPTTQEWLSTARNYAR
                     YANEGGQYDDVLAFLRQHAKE"
     gene            202..669
                     /locus_tag="VFQ61_21775"
     CDS             202..669
                     /locus_tag="VFQ61_21775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957148.1"
                     /translation="MADEYQIIDEPARSRLDHLATDPFWVLLATMLGGAWLGWPWFIV
                     NSLAVGSMHRVREISAIVVGLGVAMALTAFGLWLVMQGHLGNRALPYAMTVLVVWKLA
                     VSYWVMLWQAPSYELALHFRGVSRNGALLAFGGMFLRGMVLPALPDYWTLVLA"
     gene            680..1618
                     /locus_tag="VFQ61_21780"
     CDS             680..1618
                     /locus_tag="VFQ61_21780"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9957149.1"
                     /translation="MSFEQGLIASWLSQVSRQLQIGNLQGAIQLLKRILGMEPEHATA
                     HALLALVLIDAKRIHAALHEARLAVANDPELALAHYALGRVWIVHRDLNAARTEFELA
                     HSLDPDDSDYELGLAMVAELSGDRAAELTHLERALQLAPDSPEVLARVGQAWLERGDS
                     EKSERYAHAALEQRPDHLEALVLMGHVLLRRGQSREARDHALWGLSSNATHAGALGLL
                     VAVKARESIWLGLWWRLNSWLTQAGNRRAVVVLLVAYVVKQVSAIYTRQHGLALTSSV
                     IQYAWLALCAYTWVGPSWFARKLRAEIGKVELSKDY"
     gene            1701..2183
                     /locus_tag="VFQ61_21785"
     CDS             1701..2183
                     /locus_tag="VFQ61_21785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9957150.1"
                     /translation="MDAIRLLTDQHRQMEASLERLLKATEPERAKLFADAADLLMSHV
                     LIEEQHFYPAVKAKRTEDILLESLEEHLSLKRLLADLTALDVHNPHFEPKVHVLKEQA
                     EHHHKEEEENLFPKVSKALSSKDLDELGSRMQQAQADLLKRQPRQWAASQTRSAPDLT
                     "
     gene            3488..3949
                     /locus_tag="VFQ61_21790"
     CDS             3488..3949
                     /locus_tag="VFQ61_21790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957151.1"
                     /translation="MNSLRGERFRIVQFSVQANHIHLIVEAAERADLSNGMRALNARI
                     GKHVNCVLGRRGRLVSERHHEHSLQSPRAVRHALCYVLANFKKHPTREGRRGDPLDPY
                     SSARFFPGFAEQVNTGACAQDVPVARPRTWLLRAGWQQHGLISIYEAPRSH"
     gene            4513..5757
                     /locus_tag="VFQ61_21795"
     CDS             4513..5757
                     /locus_tag="VFQ61_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957152.1"
                     /translation="MALEPGNLSSLDGAGQEAWDELVSGWVAQLRRASPHWVAPDDPA
                     LSGAPTPVDWSGFPQRPETCLGLRKTLALLDWRTADGDLGRALGHEEYLEWRVVRGAD
                     GKLQRVEFTTEVQEYWRVLAAHHPLRLLRLAGRFAGEATAAWRDIYGELNPFATGVTP
                     AQRARAFDRNMLANNGPAKSPYNNGVKAICFLSQSVNSLAAAVGLAEFAGVPHGKREG
                     GQVVRMSAAEAIAATDTQLAVDCRSSDPTIVDVLIRLAWAGRKMSLHEPLGLYILGVD
                     PSGLALPDGSAVPLDWFEFQRGAGAAAAPGGVRLNQRLVVEAPPGSGFSLGDVIDTSL
                     DEPLRFGGQIAKHVRVGLYARLGAEGSIAVQPAVHTSTAVPACGSVSACASWRRLLEQ
                     FEAIPAPADVAQPERLTRRGPI"
     gene            5754..7034
                     /locus_tag="VFQ61_21800"
     CDS             5754..7034
                     /locus_tag="VFQ61_21800"
                     /inference="COORDINATES: protein motif:HMM:NF045168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957153.1"
                     /translation="MSDATPGTSVQTGAQEDALQAGIFFRAKERPPDHWRGVVINVSA
                     AAEKAAVRDALAQLWAMWSALGQGHVRDLARTRSDDPEVKVDGGGLTVLLGFGRSLFG
                     DERHMPILFAEDLRPPELARLMPAAPFPSLPWAKDARPLEGQGDFFVQLFGASELVVN
                     RAIVEAQKLIDDRNLPLQIVTFYRGAHREDGRSWIDFHDGVNNLRSEERRTAIEITGA
                     DPAWLRGGTFAAFLQIQVDLKAWRRLTREQQELLVGRHKLSGCPVVSATLADDRTAQL
                     AFKPGCPLRGFPTRTAQAIGPEPLEVDPDFIDPPPASGLLKTSHIHRSNFNRGSPAAD
                     GNNRIFRQGYEFLEPSGSGGLHVGLNFISFQRSLLALRRILGTTGWLGDVNFGGTAEQ
                     PLAAPPLPLMSVASGGLYAIPPRGDPFPGAPLFG"
     gene            7113..8720
                     /locus_tag="VFQ61_21805"
     CDS             7113..8720
                     /locus_tag="VFQ61_21805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957154.1"
                     /translation="MPRIRDIFFDPSMAVARLGSSSSPQVAYEWAQTPNPRVEGETSV
                     RPAWSLNVLADGTVSPFMPDRVAFRDGANIRPVCPFLEVWAVLGDSDDPATWTEAPLT
                     PALLAENGVTLSALQFVVNARNRKAERRTGLARLRYGTYPAVRVRGDEHTAVALLGRS
                     PPGSNPRMIPAGRSIPLGSFQVLRSRAQPAPNAASWASEVNVEVVRCRLTPARGFVYG
                     PPQTANPLPDGSVPVDSSRAFLEESAGWAGATVNQLVEPADTFDGAENGTSGLGASMG
                     IVDDTCEVHVEARLTLGSGVLTGRANVFVAPPDFAPDRRPFLSIADELNDRAAGQSER
                     SQRMSAAEVTAWVQDLFERVYETVSLTNVDYWRALRSISLPAAARRSAPIPGDAVPQP
                     SRAGGGLDRLRNELFPVAAPSGNQPLPLTEHARMRHRALADVDQLRDFVAQNPGRLRS
                     LVRAVFECEAAENGRATTLRMPPFMRGSNALPLTLSAWQYDLLMRWVSNIEQGGAIAD
                     LAAEGPAPLSAGAESRRREVLDRLGEP"
     gene            8717..9988
                     /locus_tag="VFQ61_21810"
     CDS             8717..9988
                     /locus_tag="VFQ61_21810"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9957155.1"
                     /translation="MRALPLLETRLPVPPEAPLYHRFQSKRGSHLLVVPYSQIYDVLG
                     DGPSESELLGLTGSYGAEAPLDAMVAPAPQAISLNVSSSCNLSCGYCYAGQGSFSGAQ
                     SGRMTAEVAETAVNRLFEGADSTRPVIVGFMGGEPFVNRTLIHHVVGFAERRARQAGL
                     EVRFSVTTNGTLLEPSDLELLRARPFAVTISVDGGAAVQDAQRPRSRLRGSFSELNRR
                     VAPLLESPGQARVSARATVSAPFSDLRSRFESLVEMGFEQVGFSPVRVSPKGALEPED
                     WPRYLEELSVVARAEAERAQRGEPIRLVNLLVALEQLRRGAASPYPCGAGGGYFSVSA
                     DGRWYACHRGVGQAEFELGDAAGLDSERRTRFLARGHVHAQAACGSCFARYLCSGGCH
                     HEASARSESSCGFIRGWLELCLATFCELEAI"
     gene            9985..11688
                     /locus_tag="VFQ61_21815"
     CDS             9985..11688
                     /locus_tag="VFQ61_21815"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9957156.1"
                     /translation="MSGVNPLKLVPRNAPAVRRASAARDGVPAAQVRRTPGNPVSSRL
                     ESGVGNCFPGLECDLRNLERRFFPFVEVDIAGNAVLVANVDEAGVSRARTSGAITSAT
                     AAAYRAIGAGGRWRITTLTGRFTALGSQTLELDTLDDRSAGSGRRPPDAWTAIRLLEE
                     GSEVTATFTRDSDGAERVITARRAAYLDADGALAISFQPGELTQSLCSPWTHDFRDCG
                     CFYWSSNHPDIAMPPVPAGELPDNRRQRPVNWQRIRTEPAFVPSAASTSDMPESEFLP
                     YYRINTAWELLNFVLDGREQLTPYEPGATTGVPFATPAELEQALRLAAGVELAVIHEY
                     LAAAYSLRDSATLSGDLRGDVRAAEAELLRIAFGEMRHLRAVNDVLQTLPRSGPFQPA
                     LRVATAIPGVVGAVQPRPAQLSTLDRFIDIERPSASVDGLYARILATLEISGTDEQEQ
                     TVRSIMGEGTDHLRTFSFIREWLARHPESAYLRPNLQRRELGNPPPEHQALQQAYLQV
                     LNDLYAGYSAGLPGGAPRINAARAAMPAPGLDRLARAVAAAGFLVEFDASTDPRFLPI
                     P"
     gene            11685..12755
                     /locus_tag="VFQ61_21820"
     CDS             11685..12755
                     /locus_tag="VFQ61_21820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957157.1"
                     /translation="MMIEADTLVIGAGPVGALAAWLRAEREQVVVAARTAFGGDAAPI
                     LEVIPGSTVALLEETLALTSGISPIEERLVAWSSAEPTELAGAPYVVDRSVLDPAILA
                     RVVSHPRVSVVPELQRDLRVGPYRANGIRAARVIDATGRSAVTATHRVSFGEPWAAWS
                     FWSARPRSVRPGLRVAALTTGYAIRISSSRHTVFSFMGPAREPLSASEWMRSVKHEAP
                     FVLEGCSFGQWERGGTRCPSLAIFDGNELRIGDASFARDALSSQGLAAGLSEALYAAA
                     VESVEDAELLRRRQRAQLADHLTSLWQAASSVRFADAPVWTGYRACLSALQRTLTPAA
                     SHAPLTALERGRITSVDAEPAA"
     gene            12825..14342
                     /locus_tag="VFQ61_21825"
     CDS             12825..14342
                     /locus_tag="VFQ61_21825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957158.1"
                     /translation="MRKATVEIDKVTGTEFPPRSVRHDGQEYSLASIFRDAGIELSVV
                     EDQADLPPPPPGQDFDLPLLHEYMSKYRKETRDGWYAHILVVPRIEYQEGWSIVKPLG
                     VMYDFRSGDLNNMPREGCAMSMSAVPADPRAYLRTLAHELGHVFNLLHPKAELPPQAI
                     DTSLMNQTADLQALGAFPDNIELSFSASNKLWLSQGPAEFVMPGGRPYTARPDDAGTL
                     VRDEALEVTSGLTLEISTRAGTFELGEPLYLRITLRNDTKQPMLVNSTLSIADGSVEV
                     WITTPSHREFRFKPVVATCHEAQSVMLPPGAEILASEPIFFGAGGHSFPAPGPYQIRA
                     RLQATASGRQLAAVSKAYAIEVLPPVSAADVDVADALASPSAALYLILRGGAHLRGAE
                     ATLSRIVREAPSRAAAQHAKLAMAAQALTPTTLNPEKAAHLLESVALERLDRTHRAEH
                     QRLLGLARACQGDERRLEAAVQQFETLAQSSRVAAESLVDDTKRWIEGIRRKTQR"
BASE COUNT         2470 a         4482 c         4918 g         2770 t
ORIGIN      
        1 gacgaggcca tcgaacgttc gctatcgcaa gccgaggagt cttcaatcac ccagggtgat
       61 ttgctgagtg cgctcggtga ggtgaagccg acgactcagg aatggctctc gaccgcacgc
      121 aactacgcgc gctacgcaaa cgagggtggc caatacgacg acgtgctcgc gttcttgcgg
      181 caacacgcca aggaatgact cgtggccgac gaataccaaa tcatcgatga gcccgcgcgc
      241 agtcggctcg atcacttggc caccgatccg ttctgggtgc tgctcgcgac gatgctgggc
      301 ggcgcgtggc tcggatggcc gtggttcatc gtgaactccc tcgcggtcgg gagcatgcac
      361 cgggtgcggg agatttccgc gatcgtggtg ggcttggggg tagccatggc gctcacggcg
      421 ttcggcctgt ggttggtcat gcaaggccac ctcggcaatc gtgcgctgcc ctatgccatg
      481 accgtactgg tggtctggaa gttggccgtg tcctattggg tgatgctctg gcaggcgccg
      541 agctacgagc tggcgctgca tttccgtggc gtctcgcgca atggtgcgct cctggccttc
      601 ggcggcatgt tcttgcgggg catggtgctg cccgcgctgc cggactattg gactctcgtt
      661 ctggcttgag ggtgcgcgcg tgagcttcga acaaggcttg attgcgagtt ggctgtcgca
      721 ggtctcgcgt cagcttcaga ttgggaatct gcagggcgcg atccaactcc tgaagcggat
      781 tctcgggatg gagcccgaac acgcgaccgc gcacgcgctc ttggctttgg tgctgatcga
      841 cgccaagcgc atccacgccg ccttgcacga agcacggctc gccgtggcca atgatccgga
      901 gctcgctctc gcccactacg ctctgggtcg cgtgtggatc gtgcatcgcg acttgaacgc
      961 ggcgcgcacg gagttcgagc tcgcccattc gctcgatccc gacgattccg actatgaact
     1021 cgggctcgca atggtcgcgg agctctccgg tgaccgcgcc gcagagctga ctcatctaga
     1081 gcgcgcgttg cagctcgccc ccgattcgcc ggaagttctg gcgcgcgtgg ggcaggcttg
     1141 gctcgagcgt ggcgactccg agaaatcaga gcgctacgcc cacgccgccc tcgagcagcg
     1201 tcccgaccac ctggaggcgc tggtcttgat ggggcacgtg ctgctcaggc gaggccagtc
     1261 gcgagaggca cgtgaccacg cgctgtgggg cttgtcttcg aacgcaactc acgcgggcgc
     1321 gctcgggctg ctggttgcgg tcaaggcgcg cgagagcatc tggctcgggc tgtggtggcg
     1381 cctcaactcc tggctgacgc aagcgggaaa ccgacgcgcg gtggtcgtgc tgctcgtggc
     1441 gtacgtggtc aagcaagtga gcgccatcta cacgcgccag catgggctag ccctcacgag
     1501 cagtgtcatt caatacgcgt ggctcgcgct ctgcgcttat acctgggtgg ggccgtcttg
     1561 gtttgcgcgc aagctgcggg ccgagatcgg caaagtagag ttgtccaagg actattaggc
     1621 gggattgccg agcgcggcag ggtggttgtt ttcgcccatg gcaggccccg agtgactggg
     1681 ccacattcga tcacagaacc atggatgcga ttcgactctt gaccgatcaa catcgacaaa
     1741 tggaagcctc tctcgagcgc ctgctgaagg cgaccgagcc agaacgcgcc aaactcttcg
     1801 ccgacgcggc ggatctgctg atgtcccacg tgttgatcga agagcaacac ttctacccgg
     1861 ccgtgaaagc caagcgcacc gaggatattc tgctcgagtc tctcgaagaa catctctcgc
     1921 tcaagcgcct attggcggat ctcaccgcgc tcgacgtcca caatccccac ttcgagccca
     1981 aggttcacgt gctcaaagaa caagccgagc accaccacaa ggaagaagag gaaaacttgt
     2041 tccccaaggt gagcaaagcc ttgagctcga aagacctcga cgagctcggc tcacgcatgc
     2101 agcaggccca agccgacctc ctgaagcgtc aaccacgcca gtgggctgcg agccaaaccc
     2161 gctccgcacc tgacctgacc tgagtacgcc agtgtgattg ggccacctcg taaagagcgc
     2221 gaccacagtc caagtgtctc gatcccgcag ccacttgact gccaattcgc tcccaacacc
     2281 gcgccggccc tcgcaataac gacactccgg atctcgttat tgtgggcggc ccgcgcggta
     2341 tcgcgacgca acgtgcggag ttgaggctga gccatcgagc gcgccgacgc gcctcgcgct
     2401 cgaaaacaaa gcggccaacg accaccaaaa caccaccacg tcacggttct cgtccctatt
     2461 gccgcaacgg gcggaagcat gtgggctggc gagcgtcggc gcggtgtcga ttgggctctc
     2521 tcgctgccat catcgcgcag gcagacgccg tctcctgggc tccccacgag gagcgatctc
     2581 cccgtacgtg gaccgtaccg taaaccacgg ctcggtctcg tccagcgcct tctgcgttgc
     2641 gtaccgatcg ccaccacttt tgacatggag gcccgatgga cttgggagcc atactgggtc
     2701 taatcaagaa tcataaacgc cctggcgagt ggcgcgcgcc tgagttgtgt cggctcgtca
     2761 cgttggtcac cgtcgcgccg cgtatgctgg gatatcagac tccactgctc ctgagacacc
     2821 ttcccacact tgggactccc tctctgctct tatcccacgc ggtgctgaac ccacgcggtg
     2881 ctgaacccac gccgcagcgt gcccttaccc tgccaccact cgttgccatt cacaagagct
     2941 tcttgtgcat aacaatgagc gctctcgttc gcgcgaacgg aacatcgccg aatcgagaac
     3001 gaggggactt cgccggtacg gctccttcgc cgatgtgtac ggcaagcact ggaacgatgc
     3061 atccgccctg gaggccccaa cgggtcaatc acaacattgc cgattcaaga tcgaccgtgc
     3121 gcgagggatg cgctggcttc gaggggcggg gagcaggagg ctacaatcct cttctcgtca
     3181 tacacaaaaa catttgtaat tggcgctcca gctgctacgc tcacgaacgc accaggaagg
     3241 ggtcggtatg tgtgtttgga agaacccacg gcagctcact ttagacctcc agcacggaca
     3301 gggagatcag cggaattggg gtggcaaacg aaaaggggcc ggtcggaagg gcggaattag
     3361 ggctgtctcg catgcgaaac ggcactccca tcgacattat caccctgtac acgtcacggt
     3421 tcgcgtctcc gtgcgttgtt tgcgagcgca gcgggtcttc ggcgtgattc gtcatgccat
     3481 tagtgccgtg aattcactgc gtggcgagcg cttccgtatc gtgcagttct ccgtgcaagc
     3541 caatcatatc cacctaatcg tcgaggcggc ggagcgcgca gacctgagca acgggatgcg
     3601 cgcgctgaat gcccgcattg gcaaacacgt caattgtgtt ctcggtcggc gtggtcgcct
     3661 cgtgtccgaa aggcatcacg agcactcgct tcaaagcccg cgcgcggtcc gtcacgcgct
     3721 gtgctacgtc ctcgccaact tcaaaaagca ccccactcgt gaaggtcgac gtggggatcc
     3781 actggatccg tattcatcgg ctcggttttt ccccggtttc gcggagcaag tgaacactgg
     3841 ggcgtgcgcc caagacgttc cggtggcgcg cccacgcaca tggctactgc gcgcgggttg
     3901 gcagcagcac gggctcatct ccatatacga ggcgccgaga agtcactgat gcatgcgtct
     3961 tgtgcccaca gtgaataaac cagtttgtca ggtcgccgga gcgtatgccc aagtttcgct
     4021 cgtgaatcgt gtggtgacct aagttccatc aaggttcgat attggtcgcg gtcagccttg
     4081 tgagctgcga aggcacgccc gcacggatcg ctctgcaagc gtcgagtctg gtgtgggcgg
     4141 tgcccgctgg gcgtccatcc gtataccctg cagctcgaac tgtcgctgcc cgaggacccg
     4201 atacactagg cctccgcgcg agctaggcct ccgcgctgga gcattcaatc gtttccgttc
     4261 cgcgccgcgg gcgcaggctg gccaacttgc gacgggtgac gcgggagaca aaggcaaagg
     4321 ctcgatgtag ccaacagcct aacgtagcct ttcagtgcaa gaactcgcgc ataggaactc
     4381 gatgatagga actggagcat aggattaatc tggatatagc gtgggttagt catttgttcg
     4441 gagtggcgcg gccgttcttc cgagtgtggt ctctgacatg tatgcttcgg agacagtgca
     4501 aaggaggtcg cgatggcgct agagccgggc aacttgtcca gcttggatgg ggcggggcaa
     4561 gaagcgtggg atgagctggt gagtggttgg gtggctcagc tccgtcgcgc cagcccacac
     4621 tgggtagctc cggacgatcc ggcgctttcg ggtgctccca ctccggtaga ttggagcggg
     4681 tttccgcagc gaccggagac gtgcttgggg ctgcgtaaga ctttggcgtt gctcgattgg
     4741 cgcacggcgg acggggacct cgggcgcgcg ctgggtcacg aggaatacct cgaatggcgc
     4801 gtggtgcgcg gcgcggacgg caaacttcaa cgggtagaat tcacgaccga ggtgcaggag
     4861 tattggcggg tgctcgcggc gcatcatccg ctgcgattgc tgcgcttggc gggtcgcttc
     4921 gcgggagagg cgaccgcggc ttggagagac atctatggtg agctgaatcc gttcgcgacg
     4981 ggggtcactc cggcgcagcg ggcgcgcgct ttcgatcgca atatgctcgc caacaacggg
     5041 cccgccaaga gcccctataa caacggggtg aaggcgattt gctttctttc gcagagtgtc
     5101 aattcgctcg cggcggcggt gggcttggcc gaattcgcgg gcgtgccgca cgggaagcga
     5161 gagggcgggc aagtggtgcg catgagcgca gccgaggcga tcgccgcgac ggatacgcag
     5221 ctcgcggtgg attgccgctc gagtgatccg acgattgtgg acgtgttgat tcgcctggcg
     5281 tgggcgggtc ggaaaatgag tctgcacgaa cccctcgggc tctacatatt gggcgtggat
     5341 ccgtccgggt tggctttgcc ggatggatcg gcggtcccac tcgattggtt cgagtttcaa
     5401 cgcggcgcgg gtgccgcggc ggcccccgga ggggtgcgcc tgaatcagcg cctggtcgtg
     5461 gaggctccac ccggttcggg tttcagtttg ggagatgtga tcgatacttc gctcgatgag
     5521 ccgctgcggt tcggcgggca gattgccaaa cacgtgcgag tgggcttgta cgcgcgtctc
     5581 ggcgccgagg gcagcatcgc cgtgcagccg gcggtgcaca cgagcacggc cgtgcccgcg
     5641 tgtggcagcg tttcggcctg tgcttcctgg cgacgcttgc tcgagcaatt cgaggcgatt
     5701 cccgcccctg ccgacgtggc gcagccggaa cggctcacgc gcagagggcc catttgagcg
     5761 acgcaacgcc agggactagc gtccagaccg gagctcagga ggatgccctg caagcgggga
     5821 tattcttccg agccaaagag cggcctccag atcattggcg gggtgtagtc atcaacgtca
     5881 gcgcggcggc cgagaaggcc gccgtacgcg acgcgctcgc gcagctttgg gccatgtggt
     5941 cggcgttggg gcagggccac gtgcgggacc tggcgagaac acggtccgat gatcccgagg
     6001 tgaaggtcga cggcggaggg ctcacggtgc tgctcggctt cggccgcagc ctgttcggag
     6061 acgaacggca tatgccgatc ttgttcgcgg aggacctgcg tccaccagag ctcgcccgtc
     6121 tgatgcccgc cgctccgttc cccagtttgc cgtgggctaa ggacgctcga ccgctcgagg
     6181 gacaaggtga tttcttcgtg cagctgttcg gggcgagcga gttggtcgtc aatcgtgcga
     6241 ttgtcgaggc gcagaagctg atcgatgatc ggaatctgcc tctgcaaatc gtgacgttct
     6301 atcgcggcgc gcaccgggag gatggccgaa gttggatcga ctttcacgat ggcgtcaata
     6361 acctgcgctc cgaagagcgg cgcacggcga tcgaaatcac gggagcggat ccggcgtggc
     6421 tgcggggcgg aacgttcgcg gcgttcctgc agattcaggt cgatttgaag gcttggcggc
     6481 gcctgactcg agaacaacaa gagttgttgg tcgggcgcca taagctcagc ggttgtccgg
     6541 tggtttcggc cactctcgcc gacgatcgca ccgcccagct ggcgttcaag ccaggttgcc
     6601 cgttgcgagg atttccgacg cggacggccc aggcgatagg gcccgagccg ctcgaggtgg
     6661 acccggattt catcgacccg cctccggcgt cgggtttact caagacttca catatccacc
     6721 gttccaactt caaccggggt agcccggcgg cggacggtaa caatcgcatc tttcgtcagg
     6781 gttatgagtt cctggaaccg tccggctcgg gcgggctcca cgtagggcta aatttcattt
     6841 ctttccagag atcgctattg gccttgagac gtattctcgg cactacgggc tggctgggag
     6901 acgtgaactt cggcggaacc gccgagcaac ccctggcggc tccgccactc cccctgatga
     6961 gcgtggcgag tggtgggctc tatgcgattc cgcccagggg cgaccctttc ccaggcgcac
     7021 ccttgtttgg ttagtggttc gaggtcgacg ctgttccaat gacaggttcg agtggtccga
     7081 gtcaccacgg ttcgccacaa agaggctgca cgatgccccg catacgagac atttttttcg
     7141 atccgtccat ggctgttgcg cgattgggca gtagctcgtc tccacaggtt gcatacgagt
     7201 gggcgcaaac accgaatccg cgcgtagagg gggagacgag tgtgcggccc gcttggtcgc
     7261 tgaatgtgct cgccgacggc acggtgagcc ccttcatgcc ggatcgcgtg gcctttcggg
     7321 acggggcgaa tattcgaccc gtgtgtccgt tcctggaggt ttgggccgtg ctgggtgact
     7381 ccgatgatcc cgcgacctgg acggaagcgc cgctgacccc cgcgctgctg gcggagaacg
     7441 gggtcacgct gagcgctttg cagttcgtgg tgaatgcgag gaatcgcaag gcagagcggc
     7501 gcactgggtt ggcgcgtttg cgctacggaa cttaccctgc ggtacgcgtg cgcggtgatg
     7561 aacacacggc agtggcgctg ctggggcgca gcccgcctgg ttcgaaccca cgcatgatcc
     7621 ccgcggggcg ctcgatccca ctcggtagct tccaggtgct ccggtcgcgg gcgcagcccg
     7681 cgccgaacgc ggcctcgtgg gcgagtgagg tcaatgtcga ggtcgtgcgc tgccggctca
     7741 cgcctgcgcg cgggttcgtg tacggtcctc cgcaaacggc caatcccctg cccgacggca
     7801 gcgtgcccgt cgattcgagc cgcgcgtttc tggaggagag cgcgggttgg gcgggggcga
     7861 ccgtgaacca attggtcgaa ccggcagaca ccttcgatgg agcggagaat gggacatcgg
     7921 ggctcggggc atcgatgggc atcgtcgatg acacgtgcga agtgcacgta gaggcgcggc
     7981 tcacgctggg cagcggggtg ctcacggggc gcgccaacgt gttcgtcgcg cctccggatt
     8041 tcgcgccaga tcgccgcccc tttttgtcga tcgcggacga gttgaacgat cgcgccgcgg
     8101 ggcagagcga aagaagccaa cgcatgtcgg cggcggaggt caccgcttgg gttcaggatc
     8161 tcttcgagcg ggtgtacgag acggtgtctc tgaccaacgt cgactactgg cgcgccttgc
     8221 ggtcgatttc gctccccgcg gcggcgcgcc gctcggctcc gatcccgggt gacgcggtgc
     8281 cgcagccgag tcgagctggc ggagggctcg atcgcttgcg caacgagttg ttcccggtgg
     8341 ctgcgccgtc gggcaatcaa cctctaccgc tcaccgagca cgcccggatg cgtcaccgtg
     8401 cgctggcgga cgtggatcag ctgcgcgatt tcgtggctca gaaccccggg cgcctgcgct
     8461 cgctcgtgcg cgccgtgttc gagtgcgagg cggcggaaaa cgggcgcgcc accacgttgc
     8521 gcatgccgcc cttcatgcgc ggctcgaatg cactgccgct gaccttgtct gcttggcaat
     8581 acgatctcct gatgcgctgg gtcagcaaca tcgagcaggg gggagcgatc gcggatctgg
     8641 ctgccgaagg gccagcgccg ctcagcgccg gagccgagtc gaggcggcgc gaagtgctcg
     8701 atcggctggg ggagccatga gggcactgcc gctactcgag acacgtctgc ctgttccccc
     8761 tgaggcgccg ctttatcacc gattccagtc caagcgcggt tcacacctgc tggtggttcc
     8821 gtactcacaa atctatgacg tcttggggga tggtccgagt gaatcggagc ttctagggct
     8881 taccggaagc tacggcgcgg aggcgccgct cgacgcgatg gtggcgcccg caccgcaggc
     8941 catttccttg aatgtcagtt cgtcgtgcaa cctgtcttgt ggatactgct acgcagggca
     9001 ggggagtttc tctggtgccc agtctgggcg tatgaccgcc gaagtcgcgg agacagcggt
     9061 gaatcggctg ttcgagggcg cagattcgac tcgtccggtg atcgttggct tcatgggcgg
     9121 ggagccgttc gtcaatcgaa ccctcatcca ccatgtggtg ggcttcgcgg agcggcgggc
     9181 gcggcaagca gggctggagg tgcgcttctc ggtgactacc aatggcacgc tcctcgagcc
     9241 ctcggatctg gagttgctac gggcgcgccc ctttgccgtg accatcagtg tcgatggagg
     9301 ggccgccgta caggacgcgc agcggcccag atcacgattg cgaggtagct tttcggagtt
     9361 gaatcggcgc gtggctccgc tgctcgagtc cccagggcag gcgcgcgtga gcgcgcgcgc
     9421 aacggtgtcg gcgcccttct cggacttgcg ctctcgattc gagtctttgg tagagatggg
     9481 cttcgagcaa gttggtttct cccccgtgcg ggtgtctccg aaaggcgcgc tcgagcccga
     9541 agactggccg cgttacctcg aggagctatc tgttgttgct cgcgccgaag cggagcgcgc
     9601 acaacggggc gagccgatac ggctcgtgaa cttgctcgtt gctttggaac agctgcgacg
     9661 cggcgccgca agcccttacc cgtgtggtgc tggtggcggc tacttctcgg tctcggcgga
     9721 tggtcgctgg tacgcctgtc accgcggcgt aggccaagca gagttcgaat tgggggacgc
     9781 tgcgggtttg gattcagaac gccgaacccg gtttctggcg cgaggccatg tgcacgccca
     9841 agcggcctgc ggcagctgct ttgctcgata cctttgctcg ggtgggtgcc accatgaagc
     9901 cagcgctcgc agcgagtcgt cgtgtggctt catccgaggt tggctcgagc tctgtttagc
     9961 gacattctgt gaattggagg ccatatgagt ggggtcaatc cgttgaagct cgttccgcgc
    10021 aacgcgccgg ccgtccgccg ggcgagcgcc gcccgggatg gcgtacccgc tgcgcaggtg
    10081 cggcgtactc ccggcaatcc agtgtcgtcg cggctcgaat ccggggttgg caattgtttc
    10141 ccggggctcg aatgtgacct gcgcaatctg gagcggcggt tcttcccctt cgtcgaggtc
    10201 gacatcgcgg gtaatgccgt gctggtggcg aacgtcgatg aagcgggggt gagtcgcgcc
    10261 cgaacgtcgg gagccattac gagcgctacg gccgcggcat accgggccat tggggcgggg
    10321 gggcgctggc gcatcaccac cttgacgggg cgttttacgg cgctgggttc gcagactctg
    10381 gagctcgaca cgctcgacga tcgaagtgcg ggcagcggcc gtcgtccacc ggacgcttgg
    10441 accgcgattc gtttgctcga agaggggagc gaggtaaccg cgacatttac gcgcgacagc
    10501 gacggagccg agcgtgtgat cacggcacgt cgtgcggcct atctggacgc ggacggagcc
    10561 ctcgcgatca gctttcaacc cggcgagttg acgcaatcac tgtgcagccc ctggacgcac
    10621 gattttcggg attgcggctg tttctattgg tccagcaatc accccgatat cgcgatgcct
    10681 ccggtgcctg ccggtgagct gcccgataac cgccgtcagc gcccggtcaa ttggcaacgc
    10741 attcgcacgg agcccgcctt cgtgcccagc gcggccagca ccagcgatat gcccgagtcc
    10801 gagttcctgc cctattaccg gatcaacacc gcttgggagc tcctcaactt cgtgctcgat
    10861 gggcgcgagc agctgactcc ttacgagccc ggtgcgacca ccggtgttcc cttcgcgaca
    10921 ccggcggagc tcgagcaagc gttgcgactc gcggccgggg tcgagctggc ggtgattcac
    10981 gaatacctag cggccgccta ttcgctccgc gattcggcga cgttgtcggg cgacttgcgg
    11041 ggtgatgtgc gcgcggcgga ggcggagttg ctgcgcatcg cattcggcga gatgcggcat
    11101 ctgcgcgcgg tcaacgacgt actgcaaacg ctaccgcgca gcggcccgtt tcagcccgcg
    11161 ctgcgggtcg cgaccgcgat ccccggggtg gtcggggcgg tgcagccgcg accggcccag
    11221 ctcagcactc tcgatcgctt catcgatatc gagcgtccgt ccgcatcggt ggatggcctc
    11281 tatgctcgta ttctagctac gctcgagatc tcgggcactg acgagcaaga acagacggtt
    11341 cgatcgatca tgggagaagg tacggatcac ctgcgcacct tcagcttcat ccgtgagtgg
    11401 ctcgcgcggc acccggagtc ggcatatctt cgtccaaact tgcagcgtcg ggagctcggc
    11461 aatccgccgc ccgagcacca agcgttgcaa caggcctatc tgcaggtgtt gaacgaccta
    11521 tatgctggct actcggctgg gctgcctggg ggagctccgc gcatcaatgc cgcgcgcgcc
    11581 gcaatgccgg cgcccgggct ggatcgtctg gctcgtgcgg tggctgcggc gggcttcttg
    11641 gtcgaattcg atgcttcgac ggacccgcgt ttcctgccca ttccgtgatg atcgaagcag
    11701 ataccttggt gattggtgcg ggtcccgtcg gggcgctcgc ggcctggctg cgtgcggagc
    11761 gcgaacaggt cgtagttgcg gctcgcacgg ctttcggcgg ggacgccgct ccgatcctgg
    11821 aggtgattcc aggctcgacc gtggctttgc tcgaagagac tctggcgctg acgtccggga
    11881 tctctccaat cgaggagcgg ctggtggcgt ggtcatcggc agagcccacc gagctggcgg
    11941 gcgcgccata cgtggtggac cggagcgtgt tggatcccgc cattctggcg cgcgtggtct
    12001 cacatccgag ggtgagtgta gtccccgaac tgcagcgcga cttacgagtc ggaccgtatc
    12061 gggcgaatgg gattcgagct gcacgtgtga tcgacgcgac ggggcgctcg gcggtcaccg
    12121 cgacgcaccg agtctcgttc ggggaaccgt gggcggcatg gtcgttttgg agcgctcgtc
    12181 cacggtccgt gcgtcccggg cttcgggttg cagccctgac tacgggctat gccatacgaa
    12241 tcagctcgag ccgccacacg gtgttttcct tcatgggccc cgcgcgagaa cccctgagcg
    12301 ccagcgagtg gatgcgctcg gtgaagcacg aggcgccatt cgtactcgag ggttgctcgt
    12361 tcggccaatg ggagaggggg gggacccgct gtccgagtct cgccattttc gacggcaacg
    12421 agctgcgcat tggggatgcc tcgttcgctc gcgacgcctt gagctcccag gggttggcgg
    12481 cgggcctctc cgaggcactt tacgccgcgg cggtcgagtc ggtggaggac gcggagctcc
    12541 tgcgccgccg ccaacgggcg cagctggcgg accacctaac gagtctgtgg caggcggcta
    12601 gcagcgtgcg cttcgcggat gcacccgtct ggacaggcta ccgggcgtgc ctgagcgctt
    12661 tgcaacggac gctcacaccc gccgcctcac atgcgccgct cacggcgctc gagcgcggcc
    12721 ggatcacgag cgtggatgcg gagccggcgg cgtgacgttc gaacgcagct cagcctcgtc
    12781 tcgtgagctt agcgaaggcg gagcgcgagc tcgaaggagg gaccatgcga aaggcgaccg
    12841 ttgaaatcga caaggtgacc ggtaccgagt tcccgccccg cagcgtgcgg cacgacggcc
    12901 aagagtactc ccttgccagc atctttcgag atgccggcat cgagctgagc gtcgtggaag
    12961 atcaagccga tctgcccccg ccgccaccgg gccaggactt cgatttgcct cttctgcacg
    13021 agtacatgtc gaagtaccgc aaggagaccc gcgacggctg gtacgcacat attctggtcg
    13081 tcccacgcat cgagtatcaa gaggggtggt cgatcgtaaa gcccttgggc gtcatgtacg
    13141 actttcggtc gggcgatctg aacaacatgc ctcgcgaggg ctgcgcgatg tcgatgtcgg
    13201 ccgtgcccgc cgatccacgt gcgtatctga gaacgttggc gcacgagctg ggccatgtct
    13261 tcaacctctt gcaccccaag gcggagctgc cgccccaggc catcgatacg agcctgatga
    13321 atcaaacggc ggacctgcag gcgctcgggg cgttccccga taacatcgag ctctccttca
    13381 gtgcgtcgaa caagctctgg ctctcgcagg ggccggccga gttcgtgatg cctggtggcc
    13441 gcccgtacac cgcgcgtccc gacgacgcgg ggaccttggt gcgcgatgaa gcgctcgagg
    13501 tgacttccgg tttgaccctc gaaatctcga cgcgagcggg caccttcgag ctcggtgagc
    13561 cgttgtatct ccgcattacg ctgcgcaatg acaccaagca accgatgctc gtgaattcga
    13621 cgctctcgat cgcggacggc agcgtcgaag tctggatcac gactccgagc catcgtgaat
    13681 ttcgcttcaa acccgtagtg gccacttgtc acgaggctca gagcgtgatg ttgccgcctg
    13741 gcgcggagat tcttgcgagc gagccgatct tcttcggcgc cggggggcac agcttccccg
    13801 cacccggtcc ctatcagatc cgagcgcgtc tgcaggccac cgcgagcggg cggcaactcg
    13861 ctgcggtatc gaaagcttat gccatcgaag tgctgccgcc ggtgagcgcg gccgacgtcg
    13921 atgtggcgga cgccctggct tcccccagcg ccgcgctata cctcattttg aggggaggcg
    13981 cccacttgcg cggagcggag gcgacgctat cgcgcatcgt tcgggaggcc cccagccggg
    14041 ccgcggcgca gcacgccaag cttgcgatgg ctgcgcaggc actcacgcca acgacgctca
    14101 atccggagaa ggccgcgcat ctcctcgaga gcgttgcgct cgaacggctg gatcggaccc
    14161 accgcgccga gcatcaacgc cttttggggc tcgccagggc ttgccaaggg gacgagcgca
    14221 gactcgaagc ggcggttcaa caattcgaga cgttggccca gagctcgcgt gtagcggcgg
    14281 agagtttggt cgacgacacc aagcgctgga ttgaaggaat ccgacgtaag acccagcggt
    14341 gaaacaccga tttgtcgcgc cgcagcctgg ctggtacaag gggcgaatgc ggtggttcga
    14401 cgtgcttacc gagtgtgcca atcccggcgg gctcgggtgt ggtggttgag tgttcttggg
    14461 ttggctgcct gcaatcaagt actcggcaat gatccgggcg ttctcagtaa acgttcgggg
    14521 cacgcgggga gcatgtccgg aggccatgca gacgcaggcc atgggaacct gggcggagcc
    14581 gccggcgacg gtgggcgcga atcggatcat gctggcgagg ctggagccct caattccaag
//
LOCUS       DASTKU010000623         8417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1460300, whole genome shotgun sequence.
ACCESSION   DASTKU010000623 DASTKU010000000
VERSION     DASTKU010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460300"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..3068
                     /locus_tag="VFQ61_21830"
     CDS             72..3068
                     /locus_tag="VFQ61_21830"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957159.1"
                     /translation="MPTLSSNIVKRDIASVHDVEKALARQALYGGDLPTNLLEVAAVS
                     EADLTRIIAETSGLQSAPVGELPRAEDAVLSTIASSVAQRYGLYPLSKEGNALRVAVS
                     EPLLPAIVAELEFSLGVKLVQQATTRVRVEQAIARDYGLELNRRVARVLAKVEGDRDP
                     YPTMPPETAASRDPSFELGTESLREASTKVAPPRTAAPETTTGIVPPSLSSPPASLLE
                     TPSSEAFSPEPPQSEVISLPGVAPSSGGAPQSTLTVRDTTNTVSDAFVGSARNSSGAS
                     RSGPDSRDSAPPGPRSGPTRAGETLRSSRPPPKLDTRTLTGSNLSQRRRRRLGPYTLA
                     MAERDLAEAISRDDVVRTFFDFASQYFEYAALFAVHRDLAEGRDAHGSGAPREKVLAI
                     GVPLDLPSTFAQVIGSESYRITRLSPTGIDGALAKDLERRPGPSTLILAIRVRGRSVL
                     ALYGDHGDQDVELPLIGDVIAFAPLVQTALERIIRLRKDRARPASAPPAAVAEVPAAS
                     ADSPESAVPNTVQEQLPSPVNGTSSDVQATLPSEPPTRSHSLPPLPMISVGGARPEAP
                     RASVVPAVLTPSQVPPRVSVLPLPSLSRTPVPSSLAPGAPPQASSNAVAVANTEPHLS
                     AAEIARAAEPEPVGIPETLRGPSMVPPPLPEDAFSAVEAPTVTGSWDSDTEESADLGS
                     RAFALSPRTPPAAQATEELQLPTVIVDLAHDTEELVGRMLEGDEAAAVRLVQMGTAAV
                     PALVGAFPGPITSELRRGAGDGPPRASECGPLLKVLARIGHKAAPILAVRSNDRSPAV
                     RAWATRLLGEMPCMDAARAIGRRFVDEDPDVRRAALAAGRMLQAHPGANAVLAATLSE
                     MLLDPAGEEHLEHMVIEAIADLREPRPIPSLIQKLAHGSSDVQRSAQWALVVLTRTDF
                     GTDTAAWTRWWEHNGSRHRIEWLIDALMHETPELRRSAGDELKSLTKEYFGYYDELPP
                     RERERAQQRYRAWWESKGKDRFSVIP"
     gene            3200..3400
                     /locus_tag="VFQ61_21835"
     CDS             3200..3400
                     /locus_tag="VFQ61_21835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957160.1"
                     /translation="MTEEETLGPALRAANTALSGRKHREVTRLASIELRFLDAQSGPW
                     EAAAMVCVGVVSLLGVLALWFW"
     gene            complement(3529..4077)
                     /locus_tag="VFQ61_21840"
     CDS             complement(3529..4077)
                     /locus_tag="VFQ61_21840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957161.1"
                     /translation="MNRLVFLSLGVFLAACGPSLEPDRVKSPDEIVAEQEALGAEQQK
                     KGRDSGDYAEPSGATDDEKRQQWDVHYAALELRRASRSAETCPESVTEKTPKGKAHVT
                     LVFGNDGHVKDKKIDDPYTETAAGKCVLRAMGAVIVKSYEGTERTVEYEVDLTGGKKS
                     GPLGTQKEEEDKYNAEVEKNGQ"
     gene            complement(4214..5578)
                     /locus_tag="VFQ61_21845"
     CDS             complement(4214..5578)
                     /locus_tag="VFQ61_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9957162.1"
                     /translation="MRRVLVVDDEENIRVVLRTLLRRHSYEVEIAASGEEALGLVDSF
                     GPDVVLTDVRMPRMGGLDLLQTLRAKGNDATVIVMSAYGNMDQAIEAMKAGAYDYVQK
                     PFKPDEIVLALRKAEEREVLRRENRALRDEIRKEHRFEDILAKSARMQEIFRTITKIA
                     DYKTTVLVTGESGVGKELVARAVHRRSSRRGAPFVAVNCGAIPENLLESELFGHKKGA
                     FTDAVQDRRGLFEEAHTGTLFLDEIGELPLALQVKLLRVLEDERIRRVGDSRDVQVDV
                     RIVAATHRDLTAETKAGRFREDLFYRLNVLHIHCPALRERREDIPLLIDHFLSRNNAR
                     LGTTIRGLDTEARRLLYEYAWPGNVRELENTIERAMVLAEGEQITASDLPERIREARD
                     PVQLQLASGELSVKKTMRVIEEILIRRALQKTKGNRTRAAEVLEISHRALLYKIKDYQ
                     IGDL"
     gene            5714..6928
                     /locus_tag="VFQ61_21850"
     CDS             5714..6928
                     /locus_tag="VFQ61_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HET9957163.1"
                     /translation="MLCGLDQLSGNQYSAQRRRLRDSRVGLLTHSAAIDRHGRSALAV
                     LEELGVAPKLIFAPEHGFDAVQQAEEPVLSRASTPEFGVNAPVLSLYGTRKEDLSPRE
                     EHLAEIDVLVIDLCDVGSRYYTYVWTALLSARAARSSGVHTLVLDRPNPISGDPSSVE
                     GAPQSPDFLSFVGLEPLPIRHALTLGEILAWFFVQDGHEVGPDTSLSVIPTRGWERLR
                     TAEAWGRPFVGPSPNMPTLETALVYPGACLIEGTNLSEGRGTTTPFQTVGAPFLNGPE
                     LARELEATGVLGAWIRPVSFRPTFEKHAGHVCHGVTLHVTNPQLFRPVATYLSLIAIA
                     RRMAGEHFAFRHSPYEFESDRPAFDLLTGSDRARLAIQDGVSPEELVSMICPVDPAYR
                     DLVLQAEARILG"
     gene            complement(6935..8308)
                     /locus_tag="VFQ61_21855"
     CDS             complement(6935..8308)
                     /locus_tag="VFQ61_21855"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET9957164.1"
                     /translation="MWPSESPVSAFIERRQRLQALLDRPAVLVSGFARPRNFTANPYP
                     FRAESHFLYLVGRHLEGAVLLVDGKGSTLFAEEADPEEELWTGRMPSLEELAKSCGLP
                     VRPLEELDVPAHALTLPAQDWEGALLQSEILERDIEPGSGPELEGADVKLADAMIQLR
                     LTHDRAAISQLEQASAATALAHSAGLAATRAAEREAQIRGAMEGAISACGCVPSYNPI
                     VTVHGEVLHQQHSNGAIRAGDLLLADVGAETPEGFAGDVTRTWPVSGKFSASQRELYE
                     VVLDAQLAAIAAVKPGARFLDVHRTASRSLVDGLLALGILRGDRAELQERGAAALFFP
                     HGIGHLIGLDVHDMEDLGDRAGYAPARKRSTAPSDRYLRLDRDLVPGMAVTIEPGFYQ
                     IARILERPEEVGELERFLCRDVLARYSDVRGIRIEDDVLVTERGCRVLTEAIPKTVAD
                     VEAALAG"
BASE COUNT         1484 a         2792 c         2556 g         1585 t
ORIGIN      
        1 tcatgtgttg acaggcgctg aagccccgtg aacattcgcc aacgaagggt atctgccttc
       61 ggctgccgcc tatgccgacg cttagctcca acatcgtcaa acgagacatc gcctctgttc
      121 acgacgtgga aaaagccctg gctcgtcagg ccttgtacgg cggtgacctc cccacgaacc
      181 tgctcgaggt agcagcggtc agtgaggcgg atctcacgcg catcatcgca gagacctccg
      241 gactgcaatc tgcacccgtc ggtgagctcc cccgcgcgga ggacgccgtg ctgagcacca
      301 tcgcgagcag tgtcgcgcag cgctacggat tgtatcctct gagcaaagag ggcaacgcgc
      361 tcagggttgc cgtgagcgag ccgttgcttc cggcgatcgt tgcggagctg gagttctcgc
      421 ttggcgtcaa gctcgtgcag caggccacga cgcgcgtgcg cgtggagcaa gcgattgctc
      481 gggactacgg gctggagctg aaccgccgag tcgcccgcgt attggccaaa gtcgagggag
      541 atcgcgaccc ttacccaacc atgcctcccg aaacggccgc gtctcgggat ccgtcgttcg
      601 agttgggtac ggagagtttg cgcgaggctt ctaccaaggt cgcgcctccc cggaccgcag
      661 ctcccgagac caccaccggg atcgtaccac cttcgctgag ttcgccccct gcctcgttgc
      721 tcgagacacc gagctccgaa gcgttttcgc cagaacctcc gcagtcagag gtaatctctt
      781 tgccaggtgt cgcccccagc tctggcggcg ctccccaatc cacgctgacg gtgcgcgata
      841 cgacgaacac cgtgagcgac gccttcgtag ggtccgcacg caactcttcc ggagcgtcac
      901 gctccggacc cgattcgcgg gactcggcgc ctcccggacc tcgctccgga ccaacgcgcg
      961 cgggagaaac actacgcagt tcgcggccgc caccgaagct cgacaccaga accttgactg
     1021 gttcgaacct gagccagcgc cgacgtcggc gcctcgggcc ttatacgttg gccatggccg
     1081 agcgcgacct tgcggaagcg atttcgcgcg acgacgtggt gcgcacgttc ttcgactttg
     1141 cctcccaata cttcgaatat gcggccttgt tcgccgtgca tcgcgatctc gccgaaggcc
     1201 gggacgcgca tgggagcggc gccccgcgcg agaaagtgct cgcgatcggg gtacctctcg
     1261 acctgcccag caccttcgcg caggtcattg ggtcggagag ctatcggatc actcgccttt
     1321 ccccgaccgg catcgatggc gcgctggcca aggacttgga gcgtcgcccc ggtccaagca
     1381 ctctgatttt ggcgattcga gtgcgcggtc gatccgtgct ggcgctgtac ggcgaccatg
     1441 gcgatcaaga tgtcgagttg cccttgatcg gcgacgtcat cgccttcgct ccgctagtgc
     1501 aaaccgcgct cgagcgcatc attcggcttc gaaaggaccg cgcgcgaccc gcgtctgcgc
     1561 cgcccgccgc ggtcgctgaa gtccctgccg cttcagcgga ttcgccggag tcggctgtgc
     1621 ccaacacggt ccaggaacag ctaccttcgc ctgtcaacgg gacgagctcc gacgtccagg
     1681 cgaccttacc ctcggagccg ccgactcgtt cacactcttt accgccctta cccatgattt
     1741 cggttggcgg agcgcgacca gaagcaccgc gcgcgagcgt cgtccccgcg gtgctgactc
     1801 cgagtcaggt gccaccgcgc gtcagcgtat tgcctttgcc ttcgctgtcg cgcacgcccg
     1861 taccctctag cctcgcgcca ggggccccac ctcaagcctc gtccaatgcg gtggccgttg
     1921 ccaacacgga gccacacctc tcggccgccg aaatcgcgcg agcagcggag ccggagcctg
     1981 tcgggattcc cgaaacgctg cgcgggccga gcatggtccc ccctcctctc cccgaagatg
     2041 ctttcagcgc cgtcgaggcc ccaaccgtta cgggtagctg ggacagtgac acggaggaga
     2101 gtgcggatct gggctcacga gcattcgcgc tgagcccgcg cacccctccg gccgcgcagg
     2161 ctacggagga actccagctc ccaaccgtga tcgtggatct cgcgcacgac acggaagagc
     2221 tcgtgggacg catgttggag ggagacgagg ccgcagcggt gcgcctcgtg cagatgggca
     2281 cagcggctgt tcccgcgctg gtcggagcct ttcctggtcc aatcacgtcg gagcttcgtc
     2341 gcggtgccgg tgatggaccg ccacgcgcgt ccgaatgcgg tccgttgctg aaagtgctgg
     2401 cacgcattgg acacaaggcg gcgccgattt tggcggtgcg cagcaacgat cgtagccccg
     2461 cggttcgcgc gtgggcgact cgcttgctcg gcgaaatgcc ctgcatggac gcggcccgcg
     2521 ccatcggtcg gcgcttcgta gatgaagacc ccgacgtgcg ccgagcggcg ctcgcagccg
     2581 gtcgcatgtt gcaggcccat ccgggagcca acgcggtgtt ggcagcgacg ctctccgaaa
     2641 tgctactcga ccccgccggc gaagagcacc tcgagcatat ggtgattgag gcgatcgcgg
     2701 atttgcgcga gccccgccct atcccttcct tgattcaaaa actcgctcat gggtcgagcg
     2761 acgtgcagcg ctccgctcag tgggccttgg tggtgctgac gcggacggac ttcggcaccg
     2821 acaccgcagc ttggacgcgc tggtgggaac acaacggctc gcggcatcgt atcgagtggt
     2881 tgatcgacgc gctgatgcac gaaactccgg agctacgccg cagcgcgggt gatgagctca
     2941 agagcctcac gaaagagtat ttcggctact acgacgaact gccgccacgc gaacgcgaac
     3001 gcgcgcagca acgttatcga gcttggtggg agagcaaagg caaagatcgc ttttccgtca
     3061 tcccttgagc cggcgtactc ccccgcgcgg agccccagcg gtacggtgat tcctcacaga
     3121 atcagccgag tattacagtc gacactgaac gatcgattgg ggtcgacacc ggctgcaaaa
     3181 tgcggcaaaa gcgggcctga tgacggaaga ggaaactctc ggccccgccc tgcgagcagc
     3241 aaacacggct ctctcgggtc gtaagcaccg agaggtaacg cgccttgcat cgattgagtt
     3301 gcgatttctc gacgcgcaaa gcgggccttg ggaagccgcc gcgatggtgt gcgtcggcgt
     3361 cgtctcgttg ctcggcgtgc tcgcgctctg gttttggtag ccaggtctgg ttttggtagc
     3421 caggggagag gccggggctc gggctaacgc aggcggctca tcgagcaccg cccgtcttct
     3481 gggacggtgc tcgatctcca agtccgcgtg cgccgcatca gaactggctc attgcccgtt
     3541 cttctcgacc tcggcgttgt acttgtcttc ttcttccttt tgagtgccga gcgggcctga
     3601 cttcttgccg cccgtgagat cgacctcgta ctcgaccgtt cgttcagtgc cttcgtagct
     3661 cttgacgatt acggcaccca tggcgcgaag cacgcatttg ccggcagccg tctcggtgta
     3721 cggatcatcg atcttcttgt ctttcacatg cccgtcgtta ccgaacacca gagtgacatg
     3781 agccttacct ttgggcgttt tctcggtcac ggactccgga caggtctccg cggagcgtga
     3841 cgcgcgacgc agctcgagcg ccgcgtagtg cacgtcccat tgttggcgct tttcatcgtc
     3901 ggtggcgccg ctgggctcgg cgtagtcgcc cgagtctcgc cccttcttct gctgctccgc
     3961 gcccaatgct tcttgctcgg ccacgatctc atcaggagat ttgacccggt ccggctcgag
     4021 tgacggaccg catgcagcca agaacacgcc aagacttagg aaaacgagcc gattcatggc
     4081 gcgcgatact acatcactcg cgcccatcct ggccaaggcg taacgcacta tttcgacttc
     4141 gggccgtctc gcgattcaga gcgtcaggag tcatctgccc caatcgcgac cccgatatcg
     4201 accccgagcg gcgctacaaa tcgccgattt ggtagtcctt gatcttgtac agcaaggccc
     4261 ggtgactgat ttccagcact tcggcagcgc gagtccggtt gcctttggtc ttttgcagcg
     4321 cgcgccggat taggatttcc tcgatcaccc gcatcgtctt cttcacgctg agctcgccgc
     4381 tcgcgagctg caattgcact gggtcccgtg cttcgcggat gcgctccggc aagtcggagg
     4441 cagtgatttg ctcgccctcg gccagcacca tcgcgcgctc aatcgtgttc tcgagctcac
     4501 gtacgtttcc cggccacgca tactcgtaaa ggagtcgacg cgcttccgta tcgagcccac
     4561 gaatcgtcgt gcctagtcgc gcgttgttac gagacaagaa atgatcgatg agcagcggaa
     4621 tgtcctcgcg tcgctcccgc aaggccggac aatggatgtg cagcacattc agccggtaga
     4681 ataagtcttc gcggaaccgc cccgccttgg tctcggccgt gaggtctcgg tgagtggcgg
     4741 cgacgatccg gacatcgacc tgaacatccc gtgaatcccc gacgcgtcgg atccgctcgt
     4801 cttccaaaac ccggagcagc ttgacctgca acgcgagtgg cagctcgccg atctcgtcga
     4861 gaaacagggt gcccgtgtgg gcctcttcga acaaaccgcg gcgatcttgg acggcgtccg
     4921 tgaaggcacc cttcttgtga ccgaacagct cgctctccag taggttctcc ggaattgcgc
     4981 cgcagttgac cgccacgaac ggcgcgccgc gccggctgct gcggcgatgc actgcgcgcg
     5041 cgacgagctc cttgcctacg ccgctctcgc ccgtgaccaa aaccgtggtc ttgtagtcgg
     5101 cgatcttggt gatcgtccga aaaatctcct gcatccgcgc gctcttcgcg aggatatctt
     5161 cgaagcggtg ctccttgcgg atctcgtcgc gcaacgcgcg gttctctcgg cgcaggacct
     5221 cgcgctcctc ggcctttcgc agcgcgagca cgatttcgtc cggcttgaac ggcttctgca
     5281 cgtagtcgta tgcgccggct ttcatggctt cgatggcctg gtccatattc ccgtaggcgc
     5341 tcatcacgat cacggtcgcg tcgttgccct tggcacgcag ggtctgaagc aggtccaaac
     5401 cgcccatgcg cggcatacgc acatccgtga gcactacatc gggtccgaac gaatcgacca
     5461 gaccgagcgc ttcctcgccg ctcgccgcta tctcgacctc gtagctgtgt cttcgcagca
     5521 aggtgcgcag caccacacgg atgttttcct cgtcatcgac aactagaact cggcgcactg
     5581 tgaaaaaaat gctagtcgcg cccgcagaac cccgcaactg tttacacgcc ctacgcaaag
     5641 tacccgaatt cgctagcaat ctgccggctt agttgactag tctgtcgcag ccgtattcgg
     5701 ctcggcgaac gccatgctgt gtggtctcga tcaactctcc ggtaaccaat attccgctca
     5761 acgacggcgc ctgcgcgatt cacgcgttgg cctgctcacc cattcggccg ccatcgaccg
     5821 ccatggtcgc agcgcgctcg cggtactcga ggagctcggg gttgccccta agctgatctt
     5881 cgcccccgag cacggtttcg acgccgtgca gcaagcggaa gaacccgtgc tctcacgagc
     5941 gagtacgccc gagttcggag tcaacgcgcc ggtcttgagt ttgtacggca cccgcaagga
     6001 ggacctgtct ccgcgcgaag agcacctggc ggagatcgat gtgctcgtga tcgacctgtg
     6061 tgacgtgggt agccgctact acacgtacgt ttggacggcc ctgctctcgg cgcgagcggc
     6121 gcgaagcagc ggtgttcaca cgctcgtgtt ggatcgtccg aacccgatct cgggtgatcc
     6181 gagcagcgta gaaggagcgc cacagtctcc ggatttcctg tctttcgtgg gcctcgagcc
     6241 cttaccgatt cgccacgcgc tcacgctagg ggagatcttg gcgtggttct tcgtgcagga
     6301 tgggcacgag gtgggtccgg acacttcgct gagcgtgatc cccacccgcg gctgggagcg
     6361 cttgcgaact gctgaagcgt gggggcggcc tttcgtcggc ccttccccca acatgccaac
     6421 gctcgagaca gcgctggtgt accccggcgc gtgcttgatc gagggcacca atctctcgga
     6481 aggccgcggc acgacgactc cgtttcagac ggtgggcgcg ccgttcttga acggccccga
     6541 gctcgcgcgt gaactcgaag ccaccggtgt gttgggtgcg tggattcgac cggtgagctt
     6601 ccgccccact ttcgaaaaac acgccggcca tgtctgccac ggcgtaacgc tgcacgtgac
     6661 gaatccgcag ctgtttcgtc cggtagccac ttatctctcg ctgatcgcaa tcgcacgccg
     6721 catggccggc gagcacttcg cgtttcgaca ctccccttac gagttcgaga gcgaccgccc
     6781 agccttcgat ctactcaccg gctcggaccg ggcgcgtttg gcgattcagg acggggtaag
     6841 cccagaggag ctggtgtcga tgatttgccc ggtagacccg gcctaccggg acctcgtgct
     6901 gcaggccgag gctcgcattc tgggttgaag gaatctaccc ggcgagggca gcctccacat
     6961 cggccaccgt cttggggatc gcctcggtca acacccggca gccccgctcc gtaaccaaca
     7021 cgtcgtcctc gatgcggatc ccgcgtacgt ccgagtagcg cgcgagcacg tcacggcaca
     7081 gaaagcgctc gagctccccg acctcctcgg gccgctcgag aatgcgggca atctgataga
     7141 agcccggctc gatggtgacc gccattcccg gcaccaagtc acggtccaac cgcaggtaac
     7201 gatccgaggg cgccgtagaa cgcttacgcg cgggagcgta gccggcgcga tctcccagat
     7261 cttccatgtc gtgaacgtcg agcccgatca gatggccaat gccatgcgga aaaaatagcg
     7321 ccgcggcacc acgctcttgg agctccgcgc gatcgccacg caagatcccg agcgcgagca
     7381 ggccgtcaac gagactgcgg gatgccgtgc gatgcacatc cagaaagcgt gctccgggtt
     7441 tgacggccgc gatggccgcg agctgggcgt ccaaaacgac ctcgtatagc tcccgttgag
     7501 aagcgctgaa tttgccgctc acgggccacg tgcgtgtgac gtcccccgcg aaaccttcgg
     7561 gtgtctccgc gccaacgtcg gcgagcaaca gatcacccgc gcgaatggct ccgttcgaat
     7621 gctgttgatg caaaacctcc ccgtgcaccg tgacgatagg attgtacgag gggacacaac
     7681 cgcatgcgct gatggctccc tccatggcgc ctcgaatctg tgcctcgcgc tccgccgcgc
     7741 gagtggcggc gagccctgcc gagtgagcca aagcggtcgc cgcagaggcc tgttcgagct
     7801 gtgagatcgc ggcgcgatcg tgagtcagcc gcaactggat catcgcatcc gccagcttca
     7861 cgtcggcccc ctccagctcc ggtcccgatc cgggctcgat gtctcgctcc aggatctcac
     7921 tctgcagcag ggcaccctcc caatcttggg ccggtaaggt caaagcgtgt gcgggaacat
     7981 cgagttcctc cagtgggcgt acgggcagac cgcaagattt cgccaactct tcgaggctcg
     8041 gcatacgccc ggtccacagc tcttcttccg gatcggcctc ctcggcgaac agcgtggagc
     8101 ccttaccgtc gacgagcaaa acggcacctt cgaggtgacg accaaccaag tacagaaagt
     8161 ggctttctgc gcgaaatggg tacggattcg cggtgaagtt gcgcggacgc gcgaagcccg
     8221 aaacaagaac ggctgggcgg tcgagcaaag cctgcaatcg ctgacggcgt tcgatgaagg
     8281 cactaacggg gctttcgctg ggccacatgc aaggtcctcg actttctgtg cgacgagctt
     8341 tcggcagcgg cgcggcagga atccgaaccg agattcaacg aaacggatga gccgaaacac
     8401 gctgctcgaa catcgta
//
LOCUS       DASTKU010000624         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1236792, whole genome shotgun sequence.
ACCESSION   DASTKU010000624 DASTKU010000000
VERSION     DASTKU010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236792"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFQ61_21860"
     CDS             <1..213
                     /locus_tag="VFQ61_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957165.1"
                     /translation="AEVSLTAVGAHFVPMTQERPGGATSEYRAQMNSEGSPSELVAAG
                     YIWMPGTELARRPPVTSRGVSSTVLM"
     gene            268..999
                     /locus_tag="VFQ61_21865"
     CDS             268..999
                     /locus_tag="VFQ61_21865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9957166.1"
                     /translation="MSTTYAELGHNRTGIATAPQLTEEMVEGTSEFLPQLDGDQREIA
                     RVRESYAKEAEPIGSVPPPKGITPMVKVAAQGLLGMRPTQFMDKLGERLAFERTGVRL
                     YEALLSKLEAFGGFEGGPTRADLEEIMLEEYEHVSLLAEAVTKLGGDPTVMTPSADLH
                     ATISKGILEVMVEPRTTFAQCLEAILVAELADNECWEALSTMAEQAGEPELTRRFISA
                     REDEAEHLSNVRLWVSAAQNRPEDS"
     gene            complement(1068..1946)
                     /locus_tag="VFQ61_21870"
     CDS             complement(1068..1946)
                     /locus_tag="VFQ61_21870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9957167.1"
                     /translation="MAEAEPLIRNVADTAFLVAYFRAQESERPDALFVDPLASKLAGD
                     RGREIGSQVAGASMMRWSVALRTRVIDTYLTEALERGVDTIINLGAGLDTRPYRMNLP
                     ESLHWVEIDAPEVIAFKETQLDGERARCRLERIPLDLRDEEARGALFGALDARAGRTQ
                     VLTEGVIPYLQSEEVASLAADLHRMRKLDSWIIDYFSPETLAFRKRTRIHGQFQQAQF
                     KFEPADWLGFFTERGWRNLETKYLAVEGARYRRQPPLPWAARALRAVMLRFIPPERRT
                     RFDNFLAYVRLEPVRA"
     gene            2047..2454
                     /locus_tag="VFQ61_21875"
     CDS             2047..2454
                     /locus_tag="VFQ61_21875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET9957168.1"
                     /translation="MNFFTRLLSPHRDAIYGVLRIVSGVLFTFHGVQKIFGVLTQHQP
                     PVGSQIWVGGIIELVGGILIAIGLFTRCAAFVASGTMAVAYTQFHWKLQFGQSFFPAV
                     NQGELALVYAFLFLYIAAAGGGRFSVDAARKRS"
     gene            complement(2455..>2917)
                     /locus_tag="VFQ61_21880"
     CDS             complement(2455..>2917)
                     /locus_tag="VFQ61_21880"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET9957169.1"
                     /translation="MLPGGESIWQVVNWVVSLALTTLLFALMFKVVPDVKIKWRDVWV
                     GAVLTAVLFTLGKFLLALYIGKSSTTSAYGAAGSLVALVIWVYYSSQVVFVGAEFTQA
                     YARHFGAGITPSENAVSTEEPEPKAAPPGAMQGATCRAPSPQTSSPPSHKA"
BASE COUNT          566 a          924 c          880 g          547 t
ORIGIN      
        1 gcggaggtga gcctcacggc cgttggcgcg catttcgtgc ccatgaccca ggagcggccc
       61 ggcggcgcca cgagcgagta tcgagctcag atgaactccg aaggatcccc gtccgagctc
      121 gtcgcggcgg gttacatctg gatgcccggc accgaactgg cgcgcaggcc tcccgtgacc
      181 tcccgaggcg tgagctcgac cgtcctgatg tgagccgccg gcgtgaagtg caattcaatc
      241 caatgagcag actaatagga gactgagatg tcgacgacct atgccgaact gggccacaat
      301 cgcaccggaa tagcgactgc accgcagctg accgaagaaa tggtggaggg aactagcgag
      361 ttccttcctc aactcgatgg cgatcagcgc gagatcgcgc gcgtcaggga atcctacgca
      421 aaagaagcgg agcccatcgg gagcgtgccc ccaccgaagg gcatcactcc tatggtgaaa
      481 gtcgccgcgc aggggctgct cgggatgcga cccacgcagt tcatggataa actaggtgag
      541 cgcttggcct tcgagcgaac cggcgttcgc ctctatgagg ccctgctttc gaagctcgaa
      601 gcgtttggcg gcttcgaagg cggccccacg cgcgcggatc tcgaagaaat catgctggaa
      661 gagtacgagc acgtctctct gctcgccgaa gcggtgacga agttgggcgg tgacccgacc
      721 gtcatgacgc cttccgcgga tcttcatgcg accatcagca aaggcattct agaagtgatg
      781 gtcgaacccc gaaccacctt cgcgcaatgc ctggaggcca ttctcgtggc ggaactcgcc
      841 gacaacgaat gttgggaagc cttgagcacc atggctgagc aggcaggcga accggagttg
      901 acgcgccggt tcatctccgc tcgcgaagac gaggctgagc atctgagcaa cgtgcgtctt
      961 tgggtatcgg ccgctcagaa ccggcctgaa gactcctgag ctcccgcaag acgggtcatt
     1021 gcgaaccttg gctctcgctc gctcgttcga ggtgacccgt ctcccattca ggctcgaact
     1081 ggctcgagcc gcacataggc caaaaagtta tcgaagcgag ttcgccgttc ggggggaatg
     1141 aagcgcagca tcaccgcccg cagcgcgcgt gcggcccagg gtagcggagg ctgacgtcga
     1201 taccgcgcgc cttccaccgc cagatacttc gtctcgaggt tgcgccagcc gcgctcggtg
     1261 aagaacccga gccagtccgc gggctcgaac ttgaactgcg cctgctggaa ctgcccgtga
     1321 atccgggttc gcttccgaaa agccagcgtc tcgggcgaga agtagtcgat gatccaggaa
     1381 tcgagcttac gcatacggtg aagatccgcg gccaagctcg cgacttcctc gctttgcagg
     1441 tacgggatca cgccttccgt aagcacctga gtacggccgg cgcgggcatc cagtgctcca
     1501 aacaatgctc cgcgcgcttc ctcgtccctc aaatcgagcg gaatgcgctc cagccgacag
     1561 cgcgcccgtt ctccatccaa ctgcgtttcc ttgaaggcaa tgacctcggg cgcatcgatc
     1621 tccacccagt gcaagctctc gggcagattc atgcggtacg gccgcgtatc cagcccggca
     1681 cccaggttga tgatcgtgtc caccccgcgc tcgagcgctt cggttaaata agtgtcgatc
     1741 acgcgcgtac gcagcgccac gctccagcgc atcatcgaag cgcccgcgac ttgagagccg
     1801 atctcgcggc ctcggtcccc cgcgagcttg ctcgccaacg gatcgacgaa cagcgcgtct
     1861 ggccgctcgg actcttgggc gcgaaaatac gcgaccaaga aagccgtgtc cgccacgttg
     1921 cggatcagag gttccgcttc agccataccc gcgagtgtgc gtcatttgcg ccgaagatgc
     1981 atcctcttcg ggaggttgag cggttttccc cttggtgcca ccgccctgag cggctaaccc
     2041 agactcatga acttcttcac tcgtctattg agtccgcatc gcgacgcaat ttacggcgtg
     2101 ctgcgcatcg tgagcggtgt gctcttcacg tttcatggtg ttcaaaagat cttcggcgta
     2161 ctcacccagc accaaccgcc ggtcggttcg caaatttggg tgggaggtat catcgagctg
     2221 gtgggcggca tcctcatcgc cattggtctc ttcacccgct gcgccgcctt cgttgcgagc
     2281 ggcaccatgg ctgtggccta cacccaattc cactggaagc tgcagttcgg ccagtccttc
     2341 tttcctgcgg tcaatcaagg cgagctcgcg ctggtttacg cgttcctttt cctttatatc
     2401 gccgctgcgg gcgggggacg tttcagcgtg gacgccgcac gcaagcgctc ctagctacgc
     2461 cttgtgcgag gggggcgagg aggtttgggg cgagggcgct cggcaggtcg cgccttgcat
     2521 tgcgccgggc ggcgcggctt tgggctccgg ttcctccgtg gaaacggcgt tttcacttgg
     2581 cgtgatgccg gccccaaagt ggcgggcgta agcctgcgta aactccgccc caacgaacac
     2641 gacctgactc gagtaataga cccagatcac gagcgccacc aaggatccgg ctgcaccgta
     2701 cgccgaggtg gtgctcgact ttccgatgta gagggcgagg aggaatttgc ccagtgtgaa
     2761 cagcacggcg gtgagcactg ctcctaccca tacatcgcgc cacttaatct ttacgtccgg
     2821 caccaccttg aacatcaagg cgaacaatag agtggtgaga gccagcgata cgacccaatt
     2881 gaccacctgc caaatggatt cgccgcccgg caacatg
//
LOCUS       DASTKU010000625        17165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_566792, whole genome shotgun sequence.
ACCESSION   DASTKU010000625 DASTKU010000000
VERSION     DASTKU010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 17165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17165
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566792"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..810)
                     /locus_tag="VFQ61_21885"
     CDS             complement(127..810)
                     /locus_tag="VFQ61_21885"
                     /inference="COORDINATES: protein motif:HMM:NF039186.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HET9957170.1"
                     /translation="MRWLYLALDLGALLGPLALSFDRKVAFYRNYRHLLPAIGVMMGI
                     FVPLDMAFVAAGIWGFDPRYVTGLSVGNLPIEEWLFFPITSYACVFIYECLRYYVRSD
                     PLRAVHRPLLIAFAVAGAVCAITHPTQIYTSLKMGGAAGLALWVALAKRPAYLSRFML
                     MYLVSWVPFLLMNGVLTGSFIEGQVVWYSNEHILGVRIGTIPVEDSYYSLFMLLTTIL
                     VYEWRRSAA"
     gene            complement(894..1607)
                     /locus_tag="VFQ61_21890"
     CDS             complement(894..1607)
                     /locus_tag="VFQ61_21890"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HET9957171.1"
                     /translation="MASSSSSVASYPHRSLRITVALLAILYGVGTWGMLSVHRPRFIA
                     LSCVNLLFTAGLLLWNARRFTSRGALAFALAAVGGFLIEWLGVSTGWPFGAYTYTDHL
                     GPRWYGVPFVIGLNWAVLTHATQASLTQALDAPSVANQKHGSIGPWLRSALGATLMTA
                     FDGLMEPAAIALRFWVWNGGHVPTANYAAWWVISFILLRLGLALNPGLRNPAAPWVLG
                     IQALFFGFVGIEAMIANPP"
     gene            complement(1597..3069)
                     /gene="crtD"
                     /locus_tag="VFQ61_21895"
     CDS             complement(1597..3069)
                     /gene="crtD"
                     /locus_tag="VFQ61_21895"
                     /EC_number="1.3.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF042421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-hydroxycarotenoid 3,4-desaturase CrtD"
                     /protein_id="HET9957172.1"
                     /translation="MKAVVIGSGVAGLAAAIRLRVLGLDVEVYEANTYPGGKLTSLQV
                     GDYRFDAGPSLFTMPELVCELFELAGRRASDYFQYEAKEVGCHYFFPDGTFLRAYTKP
                     ERLAAEVESVLGVPRHKTRAYLRHSAKVYELVGRTFMEKPLNRLATWTNPAILRAIWH
                     LREYELNRTLHDSNVARLAHPKLVQYFDRMATYSGSNPYAAPAMLSVIPHLEHNVGTF
                     VPKGGMHAITLSLYRLAQELGVTFQFDARVTRIITRDRRAIGVEIGARAVTSDVVVCN
                     MDVAPAYRKLLAALPAPERILKQERSSSALIFYWGVRKSVPRIELHNVFFGEPYEAEF
                     DAIFGNHEICDDPTVYVNATVKNVPEDAPAGCENWFVMVNAPVDIGQDWDAIVTRTRR
                     SVLAKLERSLGEKLGAYIEAERVLDPRGIETRTSSYRGALYGASSNSKWAAFMRHPNS
                     RHDLAALHFCGGSVHPGGGIPLCLNSARIAVQGIREELGL"
     gene            3070..4215
                     /locus_tag="VFQ61_21900"
     CDS             3070..4215
                     /locus_tag="VFQ61_21900"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9957173.1"
                     /translation="MPQTLRPAMRDDEVTSGDFAANLRVLSWNVWAVPWVSPDLERRL
                     RELPVALCRPAPDIIVLQELWEAEFGVRLARALREQGFIYSHHFASTAQGQTGLFVAA
                     RFPLLEPSYVPFETGRWPHTAWHLDWLVSKGLARYRLRVAGREIALGNTHLQAQYRTD
                     SYSAERLEQATDIAEQVAQEHAPLILAGDFNSASSDPARRVLRDVAGFDDALPRAALD
                     TILVRDGAAVGLRVRAARLLEIPSYRARNGELLPLSDHAPVFVELMLGTPSKLPLSSA
                     EQARVDATRALREAAELNAHKAWRWGAAAVVLALLTAWVVRRSGWLRRAPTARFRRRI
                     LVLGGAFALALGTIWTSYLSLLYYPRLAHDLERAALRFAPPTSRGAE"
     gene            complement(4268..7036)
                     /locus_tag="VFQ61_21905"
     CDS             complement(4268..7036)
                     /locus_tag="VFQ61_21905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9957174.1"
                     /translation="MPTKVLVFESDAAFARQLESGLSNFDCAVTVVDDASIGLQTAAR
                     DRPDLILLAIELPRMNGFSVCNKLKRDPGLKDVPLIIMSSDSSEETFEQHRRLRTRAE
                     DYVHKPIAFTDLLFRIQQFVSLADAESEVSADDIIIEEDDIQEHLQESSIEADDNVTD
                     RAFDSIIEASSKPSSMPVEIMDAEVLPSVPPSASGSIPPDTFPSGSMAPPSLPTFDFP
                     SLAPLGVAAASPQIFTPSIPAGVRPATMPPLTSMPPAGMISEAPEIDEAEPLVPAEPL
                     AADSPIGADSPIGAESPMAADSLAAAPSVAPIALSAAPAAVSPSAAPPAFSGSVAPPG
                     ISTSVAPGTTSTSSEWPRSNALARAMLVEATGNGLDSDVRSGSLHPSFAPGAFESDLL
                     QAGLPNSPSASFPPGPVGVRSSLLPDRVDDADAQGLREELEKERARATELEGEARAAK
                     ARILELEDQVRRGAAREHEFQRLTHELEETRARATLGKTSSSSAREFLDLREQLNKKD
                     KELIDLRDQLMHRDRELVMSRDGALALERDRADLQDRVAELSDSVKALSNRELTLSSE
                     RDQATARAEALGERASQLDRELNTKVNELNEKLSELAELKAQFEADTRARDAREIELE
                     QKARTDLERAVAATEQLEREQARAALEAASAQAQREREAAVQSTRDEAVAQGQAALAQ
                     RESELASQHEAHIHQLEREHAEDKQRLRMEQEQALAIQDKAAQDRLLAREAELLTQQS
                     EALERQAAEHAGNIDQLRQQHAHQIEQLQQQHAGETEQRRQQHAGEIEQRERRISGLE
                     QELQVRTAERDDTRRELEKRILRIEGLDTDLAMVRGELGAKTEEARGLAARLQETEAR
                     LLETQTRLGQTEQELSDSRATLARDQAVLERAREKSQGDRGSLERAKDALAAALAQID
                     EIEGRRFE"
     gene            complement(7359..8027)
                     /gene="hisIE"
                     /locus_tag="VFQ61_21910"
     CDS             complement(7359..8027)
                     /gene="hisIE"
                     /locus_tag="VFQ61_21910"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HET9957175.1"
                     /translation="MNLKFDPQTGLIPAIAQDRLSGELRMVAYMNQLALDQTLRTGFA
                     TFFSRSRNSLWVKGETSGNRLKVARVVADCDADVVLLLVDAEGPSCHTGRQNCFFHEV
                     GSDGQLQEQAESCTPTLTELERTILARTRSTGEKSYTRYLLDAGIPKINEKIREEAGE
                     LIQALSSESDERVANEAADLLFHMCVGLRSRGVDLAAVLGVLAARTGKSGHEEKRSRK
                     PSPG"
     gene            8471..10807
                     /locus_tag="VFQ61_21915"
     CDS             8471..10807
                     /locus_tag="VFQ61_21915"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957176.1"
                     /translation="MPRTLAFRRLIPLSGVLSASLGVGACSSTPSESGAPPIDGVEQI
                     VYAVRQHTTLEGGEVKTIDVAGGMGQVMDYGRFNPGGRLELLDLRTHRIENLIEDYPT
                     ADISSLDLSFDATKVVFTMKRDPDDKYHVYVAPLSRGAGGKFEITQLSFGPYDDQNAV
                     FAAGGRIVFTTNQAYTEMGTRADEYNHGRVVPQLATMTIDGGDADRKLCSQNLSHTVT
                     LFPMQDGRIGFSRWEHLENVNDVKLFAMNPDCTNMVALGGQHGKPGNSIVQVSESQTP
                     YVYYGIVTNRENTIQAGSLVRMDTRSEADPDVFDEEKTEAQSFTHLTPNVPFDDSASP
                     EGRYRSPTELPDGNVLVSWAEGPVNDLSELILTPPDYGLYVFDVKRKRRLLIQNDEGA
                     WELYAKPVVKRTEPRDLSPRQGVTDSSVPATLGSIDITLTSLSSKHDERVSGAQFRDA
                     TLDQALAQTKRVRVIEGFSSEAANQTMFGLTMAEGAALLGEAKVESDGSWLAHVPPYI
                     PIHLQPIDEFDLAIRNQTTWIQAMPGEGRVCGGCHEDRTGNNRPAMQALTLAAGRGPE
                     NLMLPVAERQEFPWAKADNPSNPREVQRILNARCVSCHNGQTNGDVAQEFYSVSMTDR
                     ATGAQTVYQIPRLDLSDTPITVTYDRRTQAWPASYVSLFYPAALDMEMGRGATVTGTV
                     PPKWAVPSDARHSVLIEKLNVTSSESAERYAWPLGEAFTDTNVRGANRTDHAKQAGLT
                     REELVTLIRAIDLGGQYYARQNTGFMPYTSDPVSPNWP"
     gene            10821..11735
                     /locus_tag="VFQ61_21920"
     CDS             10821..11735
                     /locus_tag="VFQ61_21920"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9957177.1"
                     /translation="MNTRTRSMLSCVALGLIASSALAEPAVHAGRAEVYTSLDSDSLE
                     RVTSAKALEVELSAENVAPTRIWKLLEHGEKVECLSCIPVVSKLLFDANDKTREISAW
                     WLRRRVFGVFGPGEVYSRVVESLSNSEQSEVRRSYAANAIGEFLNPAGVPLVAKAAVE
                     DTSPRVRLAAVRALTRLNHEGPAQELGTALADSDEQVRLAALNGALGVNVFSSTPAVV
                     ERLGDGSASVRRRAAEAVGVMHLADAAMALVPLCSAATEADPNVRAAALWALGQIGDS
                     AYRSVVEGAQGDANPQVKSSARIALRRL"
     gene            11774..14851
                     /locus_tag="VFQ61_21925"
     CDS             11774..14851
                     /locus_tag="VFQ61_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957178.1"
                     /translation="MFLSELRARYWWLAALSAALPLSLAACSRDEQSPTSTFYSRKVG
                     PVLEQSCATSSTRSRCHVVADAQGNALGNLSVESFEHLSYRRDLLLNYGPYGMPGLLL
                     KVVPNFTLSLTDYKGGPPTLITTDIAHAGERLVDFGSPTFNLISRWMENGASENNAPQ
                     PERNAEQRPCNTALGSGSEFDASVDPSSADFQIFRDQVNPVLGESCAAGNCHGSRSNT
                     LYLTCGSSPEQVRWNYFAASDFTSRDPGSSDIARRALAPSAGGTYHEGGTVFPSTADL
                     GYQVILKWAEQKGGPTRIPSEPGFEFFASRVQPMLVKRGCMMLGCHSAAMFHDYRLRG
                     GSGGHFGLPATRLNYELSLLQLALESPNPNASRLIAKNLPPERGGILHRGGALFAREG
                     VAQACDATRAESGPLDEQTPYCVISAWIAKERAARMASAVPFSGIVYVKRAASPGPDT
                     PQDWERSTVAGDVVRVAATLGADGTVTLGAETSLSRQCGLDPATSQARRPAVSWDGQR
                     IAFSARSGATAAYRIYVVDGTNCEVEAAIDRAPVDSSGAPVALNGELSHNLDPAFAPD
                     GRIVFVSTRGNLQSARPTGPRRTPADPSKLDTNLYVRESDGRIRQLTYLLNQEMLPSF
                     MRDGRVILTTEKRTLGFYQLAGRRINLDGGDYHPLFAQRKSIGFQQFTSVVELPNKNL
                     VAIASERGAAHGAGALALVNRSIGIDQGSSDPKDYLVDPSAITFPNPDFFQHSLSFID
                     PAATGQLSGTRGAYFAPSAFVDGRILVSHAPDVTNLGEFSGNFDVVLVDPSRPAAAPV
                     PLITGPEDALWPVAIYARQNAGIFSSRLDEANGASEIHTELGDRAEITYMDLPLLGSL
                     LFQNTRSGRRISEGPQTFQLWESLPPEPGVTSFANAGAFVTRDQDGEVYVRRGLLGAL
                     QPYADGSAKVSLPGGVPFVVAGQFQLAEDAAPTQRFQREEMQVYPGERGRQSFQRRFF
                     EGMCGGCHGSVSGQEYQVAVNPDILTQASEVAAKDAPAIVVEQTGNVLGPEFP"
     gene            complement(14873..16564)
                     /locus_tag="VFQ61_21930"
     CDS             complement(14873..16564)
                     /locus_tag="VFQ61_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957179.1"
                     /translation="MPYAVNCNRCGARFNVSDELYRRKFVGKVVTLRCQRCNAPIRVD
                     ATDPPTNPKIPVAAPAYEVPAQKGAPKPPARALEKSGEASPPPPPVARGSLNPADSAT
                     GATNHHDLFAIDSLPPLTAGEYVDLSEGLQSEPPASSAPPLAALTRPIEPAPESVPRP
                     SDRLLNIGSNGFGGAGLGASFDPSTLPRFSTPPPDSEGSPRTPRTAFHNPFDSSKPAN
                     DVPVASSTSQAAIGHERPASEAVPGGSARVLASASDTSSGHTAPASVRRGLPVGSGRL
                     EGVGSGAADESTQHGPQRRSMRWGVAVVLAVAITAAVVSSRKTLSSGQSAGAETVIAK
                     ALQTDQHTSNEPTPSTPATRPEAAAPLAGNREGDSSAADVDASKSSSADASSRRSSAN
                     RNTETTPNGAARSSSLRSTPGAGVTGALTQAEARVIARNEPRADAARAESARTGLGSK
                     REPAPSSEPVEAEPAEPPAPAIPKGPFDRAAAASALQGAAAQAGHCRKPGDPSGVATV
                     TVTFAPSGRVTSATLSGPPFAGTATGGCIASTLRRAQVPAFDGDKITVSKTIAIE"
     gene            complement(16676..>17165)
                     /locus_tag="VFQ61_21935"
     CDS             complement(16676..>17165)
                     /locus_tag="VFQ61_21935"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HET9957180.1"
                     /translation="ILSASTGLACAVRTADCVPILIADRRSGAVAAVHAGWRGVVGSV
                     IECALRELARSQSEAPEWVAAIGPHISRAAFEVSDEVAHQLQQASPDPGVVDRSRARP
                     HVDLRRIVRAKLLALGVEASWIDDVDGCTVSDARQFFSFRRDGAQSGRHLSAIVARGR
                     QT"
BASE COUNT         2958 a         5453 c         5623 g         3131 t
ORIGIN      
        1 gttctcgctg attccgtcgg cggcaggcag cgccggagta ggctgatcgc tttgcgcggt
       61 cggtccgact cttgcggggg tgagcggcgc gctatcagtc atgggcttcg gagcggcgcc
      121 gccagttcac gcggccgagc gacgccactc gtagacgagg atggtggtca gcagcatgaa
      181 caagctgtag taggagtcct ccacggggat ggtgccaatc cgaacaccca agatgtgttc
      241 gttcgaatac cacaccactt gcccttcgat gaagctgccc gtgagcacgc cgttcatgag
      301 caggaacggc acccacgaga cgaggtacat gagcatgaag cggctcagat aggcaggcct
      361 cttcgccaac gcgacccaca gggcgagccc cgcagcgcca cccatcttca agctggtata
      421 gatctgggtc gggtgcgtga tcgcacaaac cgctcccgcc acggcgaagg caatcagcag
      481 cgggcgatgg acagctcgca acggatcgct tcgcacgtag taccgcaaac attcgtagat
      541 gaacacgcaa gcgtagctcg tgattggaaa gaagagccac tcttcgatgg gtaggttgcc
      601 gaccgagagc ccggtcacgt atcgtggatc gaacccccaa ataccagcag ctacgaaggc
      661 catgtccaag ggcacgaaga tgcccatcat gacccctatg gcgggaagta ggtgccggta
      721 attccggtag aacgccacct tacgatcgaa gctcagggcc agaggtccga ggagcgcgcc
      781 caagtcgagc gccaagtaga gccaccgcat ttgcttttcg ctcgtctcgc gtgccttctt
      841 tttcgctgat cgcgtgtgcc gcggccagga gtgcgcgtca atcgggcggc ttgctaaggc
      901 gggttcgcga tcatggcttc gatgcccacg aaaccaaaga aaagtgcctg aatcccgagt
      961 acccagggtg cggcggggtt ccgcaaaccg ggattgagcg ccaagcccaa gcgcaacaga
     1021 atgaaggaga tgacccacca cgcggcatag tttgccgtgg gtacgtgccc gccgttccag
     1081 acccagaaac gcagggcgat agcggccggc tccatcagcc cgtcgaacgc ggtcattagt
     1141 gtggctccaa gcgccgacct aagccagggg ccgatcgagc cgtgtttctg gttcgcgacg
     1201 ctcggtgcat cgagcgcctg agtcaagctg gcttgcgtcg cgtgtgtgag taccgcccag
     1261 ttcaggccaa tcacgaacgg gaccccgtac cagcgtggcc ccaagtgatc ggtgtaggta
     1321 taggcgccga agggccaccc cgtgctcacg cccaaccatt cgatgaggaa gccgccgacc
     1381 gccgcgaggg caaaggccag ggcaccgcga gacgtgaacc ggcgcgcatt ccaaagcagg
     1441 agccccgcag tgaacagcag attgacgcaa ctcagcgcga tgaagcgcgg ccggtgaacg
     1501 ctgagcatgc cccatgtgcc cacgccgtac aggatcgcga gcagggcgac ggtgatgcgt
     1561 agcgatcgat gagggtagct cgcaaccgag ctagagctag aggccaagtt cctcccgaat
     1621 accctgtacg gcaatgcggg cggagttcaa acacaacggg atgcccccgc cggggtgcac
     1681 gctgcctccg cagaaatgca gcgccgctag gtcgtgtcga gagttcggat ggcgcatgaa
     1741 cgcggcccat ttggagttcg agctcgcgcc gtaaagggcg ccccgataag aggacgtgcg
     1801 ggtctcgatc ccgcgaggat cgagcacgcg ctccgcttcg atgtacgccc cgagcttctc
     1861 acccaggctt cgctctagct tggcgagcac gctccggcgc gtgcgcgtga cgatggcgtc
     1921 ccaatcttgg ccgatgtcta ccggtgcgtt caccatcacg aaccagttct cgcatcccgc
     1981 gggcgcatct tcgggtacgt tcttcacggt cgcgttgacg tacaccgtgg gatcgtcgca
     2041 gatctcgtgg ttgccgaaga tggcgtcgaa ttcggcttcg taaggctcgc cgaagaacac
     2101 gttgtggagc tcgatgcgcg gtacgctctt gcgcacaccc caatagaaaa tcagcgccga
     2161 gctcgaacgc tcttgcttca gaatccgctc cggcgccggc aacgcagcga gcagcttgcg
     2221 ataggctggc gctacgtcca tgttgcagac cacgacgtca ctggtcacgg cacgggcgcc
     2281 gatttccacg ccgatcgccc gccgatcgcg cgtaatgatg cgcgtgaccc gcgcgtcgaa
     2341 ctgaaacgtg acccctagtt cttgcgccaa acgatacagg ctcaacgtga tggcgtgcat
     2401 gccccctttg ggcacgaacg tcccgacgtt gtgctccaaa tgcgggatca cactcagcat
     2461 ggctggcgcc gcgtaagggt tggagccgct gtacgtggcc atgcgatcga agtactgcac
     2521 gagcttcggg tgagcgaggc gcgcgacgtt ggagtcgtgc aaggttcgat tcagctcgta
     2581 ctcccgtaag tgccaaatcg cgcgcaaaat ggcgggattg gtccaagtcg cgagccggtt
     2641 cagcggcttc tccatgaacg tgcgtccgac cagctcgtag accttggccg aatggcgcag
     2701 gtaggcgcgg gttttgtggc gtggaacgcc gagcacactc tcgacctcgg cggcgaggcg
     2761 ttcgggtttc gtgtaggctc tcagaaacgt gccgtccgga aagaagtagt ggcagccgac
     2821 ctccttggct tcgtactgga agtagtcgga agcccttctg cctgcgagtt cgaacagctc
     2881 gcaaacgagt tccggcatgg tgaacagcga tggccccgcg tcgaagcgat aatcgccgac
     2941 ctgcagcgag gtgagtttac ctcctgggta agtgttcgcc tcgtatactt ctacgtctag
     3001 gccgagcacc ctgaggcgaa tggcggccgc taggcccgcg actccggaac cgatgacgac
     3061 tgctttcacg tgccgcagac tctaagacca gccatgcggg atgacgaggt cacctccggc
     3121 gattttgccg ccaatttgcg ggtgttgagc tggaatgtct gggcggtgcc gtgggtttct
     3181 ccggatttgg agcggcggct tcgggagctg ccggtcgcct tgtgcaggcc cgcaccggat
     3241 atcatcgtgc tccaggagct atgggaggcc gagtttggtg tccgactcgc tcgtgcgctc
     3301 cgggagcagg gcttcatcta ctcgcatcac ttcgcgagca cggcgcaagg ccagacgggg
     3361 ctcttcgtgg cggcgcgctt cccgctactc gagccgagct acgtgccctt cgaaaccggg
     3421 cgctggccgc acacggcgtg gcatttggat tggttggtca gcaaggggct cgcccggtat
     3481 cgcctgcgag tggcgggtcg ggagatcgcg ctcggcaaca cgcatttgca ggcgcaatat
     3541 cggaccgact cctactcagc ggagcggctg gaacaggcta ccgacatcgc ggagcaggtg
     3601 gcccaggaac acgcgccact gatcttggct ggcgatttca acagcgcctc ctccgatccc
     3661 gcgcgacgcg ttctgcggga cgtcgcaggc ttcgatgacg ccttgcctcg cgcggccctc
     3721 gacacgatct tggtgcgcga cggggcggcc gtgggcttgc gggttcgggc ggcgcgactg
     3781 ctggagatcc cgagctaccg cgcccgtaac ggtgagttgc tgccgctttc cgatcatgcg
     3841 cccgtattcg tcgagctaat gctggggaca ccctcgaagt tgccgttgag cagcgcggaa
     3901 caggcgcgcg tcgacgcgac gcgcgcgttg cgtgaggccg cggagctgaa cgcgcacaag
     3961 gcctggcggt ggggggctgc tgccgtcgtg ctcgcgctgc tcacggcttg ggtggtgcgg
     4021 cgcagcgggt ggctgcgacg agcaccaacc gcgagattca ggcgtcgtat tctcgttttg
     4081 gggggcgcct ttgcgctcgc gctcggtacg atttggacga gctacttatc gttgctttac
     4141 tacccgaggt tggcgcacga cctcgagcgc gcggcgctgc gtttcgctcc acccacgtcc
     4201 cgcggggcgg aatgaagaag ggtccgcgtc aatacgtgcg cgatggatgc tctttcgagt
     4261 gacgggacta ctcgaaacgg cgcccttcga tttcgtcgat ctgcgccagc gccgccgcca
     4321 aggcgtcctt ggctcgctcc agcgagccac gatcaccctg agacttctcg cgcgcccgtt
     4381 cgagcaccgc ttggtctcgt gccaaggtag cccggctgtc ggagagctct tgctccgttt
     4441 ggccaaggcg cgtttgcgtc tcgagcaagc gagcttccgt ctcttgcagc cgcgccgcga
     4501 gccctcgcgc ctcctccgtc ttggcgccca gctcgccgcg caccatcgcc aagtcggtgt
     4561 ccagcccttc gatgcgcagg atccgcttct ccagttcacg tcgggtatcg tcgcgctcgg
     4621 ccgtgcgcac ttggagctcc tgctccaggc cggagatgcg tcgctcgcgt tgctcgattt
     4681 cgcccgcgtg ttgctggcgc cgctgctccg tttcgccggc atgttgttgc tgcagctgct
     4741 cgatctggtg cgcgtgctgc tgacgcagct gatcgatgtt gccggcgtgc tcggccgctt
     4801 gtcgctctag ggcttcactc tgctgggtga gaagctcggc ctctcgcgcg agtagccgat
     4861 cttgagcggc tttgtcctgg atcgcgagcg cttgctcttg ctccatgcgc aggcgctgtt
     4921 tgtcctcggc gtgctcgcgc tcgagctgat ggatgtgcgc ctcgtgctgg ctcgcgagct
     4981 cactttcgcg ctgcgcgagc gcggcctggc cttgggcgac cgcctcgtcg cgcgtcgact
     5041 gcaccgcggc ctcgcgctca cgctgggctt gcgccgaggc ggcctcgagc gccgctcggg
     5101 cttgctcccg ttcgagctgc tcggtggcgg caacggcgcg ctccaagtcg gtacgggctt
     5161 tttgttcgag ctcgatctcg cgcgcatctc gggcacgagt atcggcttcg aactgggcct
     5221 tgagctcggc gagctcgctg agcttctcgt tgagctcgtt gaccttggta ttgagctcgc
     5281 ggtcgagctg gctcgcgcgt tcaccgagtg cctcggcccg ggcggtcgcc tgatcgcgct
     5341 cggaactcag cgtgagctct cggttcgaga gcgccttcac gctgtcgctc aactcggcga
     5401 cccgatcttg cagatcggct cgatctcgct cgagcgcgag cgctccatcc cgcgacatca
     5461 cgagctcacg gtcgcgatgc atgagctggt cgcgcagatc tatcagctct ttgtctttct
     5521 tgttcagctg ctcacgcaaa tccaggaact cgcgagcgga cgagctgctg gttttgccga
     5581 gcgtggcccg cgcccgggtc tcttcgagtt cgtgcgtgag tcgctgaaac tcgtgctcgc
     5641 gcgcggcgcc gcgccgcacc tggtcctcga gctccaaaat gcgggccttg gccgcgcgcg
     5701 cttcgccctc gagctcggtc gcgcgcgccc gctctttctc gagctcttca cgcagccctt
     5761 gagcgtcggc gtcatctact cgatcgggta acaacgaaga acgcacgcca accggaccgg
     5821 ggggaaacga agccgacggc gagtttggca gccccgcctg cagcagatca ctctcgaacg
     5881 cacctggcgc gaacgagggg tgcaacgaac cgctgcgcac atcggagtcc aatccattgc
     5941 cggtggcttc aaccaacatg gcgcgcgcca aggcgttcga gcgcggccac tcggagctcg
     6001 tactcgtggt gcccggggct accgacgtgg agatccccgg cggcgctacc gagccagaaa
     6061 acgctggcgg agctgccgaa ggcgagacgg ctgccggagc cgccgacagc gctatcggag
     6121 ccactgaagg cgcggcggcg agcgagtctg ctgccattgg cgactctgct cctattggcg
     6181 agtctgctcc tattggcgag tctgccgcta acggctccgc tggcacgagc ggctccgcct
     6241 catcaatctc tggagcttcg gaaatcatac ccgcgggcgg catcgacgtc agaggcggca
     6301 tggttgcggg ccgcacgccc gccgggatgg atggcgtgaa gatctggggc gatgccgccg
     6361 ccacgccgag cggcgcgagc gagggaaagt cgaacgtggg cagcgaaggc ggtgccatcg
     6421 acccgctcgg gaacgtatcc ggcgggatcg agccgctcgc ggatggcggc acggagggca
     6481 gcacctccgc gtccatgatc tcgaccggca tcgaggatgg cttggaagaa gcttctataa
     6541 tcgagtcgaa cgcgcgatcg gtaacgttat cgtccgcctc gatggagctc tcttggagat
     6601 gctcctggat gtcgtcttcc tcgatgatga tgtcgtcggc gctgacctcg gattcggcgt
     6661 ccgcgaggct cacgaactgc tgaatgcgaa acaacagatc cgtgaacgcg atcggcttgt
     6721 gcacgtaatc ttccgcgcgc gtgcgcagac gccgatgctg ctcgaacgtc tcctcgctcg
     6781 aatccgagct catgatgatg agcgggacgt ccttcaggcc cgggtcccgc ttcaacttgt
     6841 tgcagaccga gaagccattc atgcgcggca gctcgatcgc gagcaggatt agatccgggc
     6901 gatcacgggc cgctgtctgc aacccgatgc tcgcgtcgtc caccaccgtc accgcgcaat
     6961 cgaagttcga cagcccgctc tcgagctgtc gtgcgaacgc ggcgtcactc tcgaaaacca
     7021 ggactttcgt cggcatgtgg gcggggaggg tggggctaga gcggcgcccg atcgcgcctc
     7081 gtcgcacacg atatcgggcg gtcgagccgg ggtcaaacgg cagcttgccg cggcaagctt
     7141 gaacccgtgg gaccgtcaaa ggcgactcga gccggcgagt ggctcctgag cttcacggcg
     7201 gggttcgaac cgcacggccg cagcaccgcc gagcaggttc gaaccgccgg actgtttggg
     7261 cgaagggcta gcgcggtggt gcagggctag ccctgcgatg tagtccaagc cggcggggca
     7321 cgatacccag gaaacacggg gccgctgacc cagtctggtc aacctgggct gggcttgcga
     7381 ctgcgcttct cctcgtggcc actcttaccg gtgcgcgcgg cgagcacacc cagcaccgct
     7441 gccaagtcca cacctcggga ccggagcccg acacacatgt ggaagagaag gtcggccgct
     7501 tcattggcga cccgctcgtc cgattcggag ctgagcgctt ggatcagctc gccggcttcc
     7561 tcacggatct tctcgttaat cttgggaatg ccagcgtcca gcaggtaacg cgtatagctc
     7621 ttctctccgg tacttcgcgt gcgcgccagg atggtgcgct cgagctcggt gagggtcggg
     7681 gtgcagctct cggcttgctc ctgaagctgc ccgtcggatc ccacttcgtg gaagaagcag
     7741 ttctgccggc ctgtgtgaca ggacggccct tccgcgtcga ccaggagcaa gacgacgtcg
     7801 gcgtcgcagt cggcgacgac gcgcgcgacc ttcaggcggt ttccagaggt ctctcccttc
     7861 acccaaaggc tgttgcggga gcgactgaag aaggtcgcaa aacccgtgcg caacgtctga
     7921 tcgagcgcga gttggttcat gtaggcgacc atgcgcaatt caccgctgag tcggtcttgg
     7981 gcgatggccg ggatcaagcc agtttgagga tcgaatttga ggttcacgtg tcggcgcgca
     8041 cattagcacc accgcgactt cgactcctcc cctccgcgtt ccaacctgtc ttcccgcaat
     8101 ttctgagtct tggatggtct aggggagttc gagggttccg tcgaggggtc gccccaactc
     8161 gacattccca ttcagggccc actcagagcc cattccgaag agagctagtc gctcgcagtc
     8221 gacagttccg gcgctcctcg tgggacgggc tcttaaccat gtgagttgga gtatcagcta
     8281 ccagttaagt tgagtggcta cgctatagat gagggggtgc tttgcccccg cgcgggggcc
     8341 ttgccccacg tgaacacctc tgtaacatcg tgaaattgcg tgtataatca atggcccgct
     8401 tcctgctcta aagacgggtc gagagcggtg gccggttgcc agcccacgcg tgtctcgaac
     8461 aggagtgctc atgcctcgaa cgcttgcctt tcgccggttg atacctctga gtggagtgct
     8521 gagcgccagt ctgggggtcg gggcgtgctc gtctacaccc tccgagagcg gcgctccgcc
     8581 gatcgatggc gtcgagcaga tcgtatacgc cgtgcgtcag cacaccacgc tcgagggcgg
     8641 agaggtcaag acgatcgacg tcgcgggcgg catgggccaa gtgatggatt acggtcgttt
     8701 caaccctggc gggcgattgg agctgctcga tttgcgcacg caccgcatcg agaacctgat
     8761 cgaggactat cctacggcgg acatatcgag cctcgatttg agcttcgacg cgaccaaggt
     8821 cgtgttcacg atgaagcgcg acccggacga caagtatcac gtctacgtcg ctcctttgag
     8881 ccggggcgcc ggaggcaagt tcgagattac gcagctcagc ttcggtccct acgacgatca
     8941 aaatgcggtg tttgccgcgg ggggccgcat cgtcttcacg accaatcagg cctataccga
     9001 gatgggcacc cgcgccgatg agtacaacca cggtcgggtc gtgccgcagc tcgccacgat
     9061 gacgatcgac ggcggagacg ctgatcgcaa gctgtgctca cagaatctct cgcacaccgt
     9121 caccctgttt cccatgcagg atgggcgcat cgggttctcg cgctgggaac acctcgagaa
     9181 cgtcaacgac gtgaagctct tcgcgatgaa ccccgactgt accaacatgg tcgccctcgg
     9241 cgggcagcac ggtaagccgg gtaactcgat cgtgcaggtg agcgagtcgc agacgccgta
     9301 cgtgtattac gggatcgtca ccaaccgcga gaacaccatt caagccggca gcttggtgcg
     9361 catggatacg cgctccgagg ctgatcctga cgtgttcgat gaggagaaga ccgaggctca
     9421 atccttcacc cacttgaccc cgaacgttcc gttcgatgac tcggcctcgc ccgaggggcg
     9481 ttatcgttcc ccgacggagc tgcccgacgg caacgtgctg gtgtcttggg cagaggggcc
     9541 cgtcaatgac ttgagtgagc tgattctcac tccgccggat tacgggcttt acgtgttcga
     9601 cgtgaagcgc aagcggcgct tgttgattca aaatgacgaa ggcgcctggg agctctatgc
     9661 caagccagtg gtgaagcgca ccgagccgcg tgatttgtca ccccggcaag gtgtgacgga
     9721 ctcgagcgtg ccggccaccc tgggctcgat cgacatcacg ttgacgtctc tgagctccaa
     9781 gcacgacgag cgcgtcagcg gagcgcagtt tcgcgatgcg acgctcgacc aggcgctggc
     9841 gcagaccaag cgagtgcgcg tcatcgaagg cttctcgagt gaggccgcca atcagaccat
     9901 gttcgggctg acgatggcgg aaggggcggc cttgctcggc gaggccaagg tcgaatcgga
     9961 cggttcctgg ctcgcccacg tgccacccta tatcccgatt catttgcagc cgatcgacga
    10021 gttcgatctc gcgattcgca accaaaccac ctggattcaa gccatgcctg gcgaagggcg
    10081 ggtgtgcggc ggctgtcacg aggatcgcac cggcaacaat cgtccggcca tgcaggcact
    10141 cacgctggcg gcgggccgcg gccccgagaa cctcatgctg cccgtcgcgg agcgccagga
    10201 gttcccttgg gccaaggcgg ataacccgag caacccacgc gaagtccagc gcatcctcaa
    10261 cgcgcgctgt gtgagctgtc acaacgggca gaccaacggg gacgtggccc aagagttcta
    10321 ctcggtgagc atgaccgatc gggcaacggg cgcgcagacc gtgtaccaga ttccgaggct
    10381 cgacctgagc gacactccga tcacggtcac ttacgatcgg cgcacccagg cttggccggc
    10441 gagctacgtc tcgctgttct atccggccgc gctcgacatg gaaatgggcc gtggcgcgac
    10501 ggtgacgggc acggtgccac cgaaatgggc ggtgccgagc gacgcgcggc acagtgtgct
    10561 gatcgagaag ctgaacgtca cctcgagtga gagtgcggag cgctatgcgt ggccgctcgg
    10621 cgaggctttc accgacacga acgtgcgtgg cgcgaaccgc accgatcacg cgaagcaagc
    10681 cgggctcacc cgcgaagagc tagtcacctt gattcgagcc atcgatctgg gtggccaata
    10741 ctacgcccgt cagaatacgg gcttcatgcc ttacacctcg gatccggtct cgccgaattg
    10801 gccctgaaca cgagcaaacc atgaacacac gaactcgttc gatgctttct tgtgtagcgc
    10861 tagggctgat cgccagctcg gcgctcgcgg agccggccgt gcatgctggg cgcgccgagg
    10921 tttacacgtc gctcgacagc gactcactcg agcgcgtaac gagcgcaaag gcgctcgagg
    10981 tcgagctctc cgcagagaac gtcgcgccga ctcggatctg gaagctgctc gagcacggcg
    11041 agaaggtgga gtgcctgagc tgtatcccgg tggtctcgaa gctgctgttc gacgccaacg
    11101 acaagacgcg cgagattagc gcttggtggt tgcggcgccg cgtgttcggg gtgttcggac
    11161 cgggagaagt ctactcgcgc gtggtagaga gcctctctaa ctcggaacag agcgaagtgc
    11221 ggcgcagcta tgccgccaac gcgatcgggg agttcttgaa ccccgcgggg gtgcctctgg
    11281 tggccaaggc ggccgtcgaa gacacgtcgc ctcgcgtccg cttggcggcc gtgcgagcgt
    11341 tgactcgtct gaatcacgaa gggccagctc aggagctggg gaccgcgctc gccgactcgg
    11401 acgaacaggt tcggctcgcg gctctcaacg gggccctcgg cgtcaacgta ttctcgagca
    11461 cgccggcggt ggtggagcgc ttgggtgacg gctccgcgag cgtgcgccga cgagcggcgg
    11521 aagcggtggg agtaatgcac ctggctgacg cagccatggc gctggtgccg ctgtgcagcg
    11581 ctgcgaccga ggcggatccg aatgtgcgtg cggccgcgct gtgggcactc ggacaaatcg
    11641 gcgattccgc ctaccgctcg gtggtggaag gtgcgcaggg agacgccaac ccgcaggtca
    11701 aaagttcggc tcgcatcgcg ttgcgtaggt tgtagtgcct tccgcctgat attgtgttcg
    11761 cattactccc cgcatgtttt tgtctgaact gcgcgcgcgt tactggtggc tggcggccct
    11821 gagcgccgcg ctacctttgt cactagcggc gtgttcacgt gacgagcagt cgcccacctc
    11881 gactttctat tcgcgcaaag taggtccggt gctggagcag tcctgcgcga cgagctcgac
    11941 gcgatcgcgc tgccacgtcg tcgccgacgc tcaaggtaac gccctcggca acctgagcgt
    12001 cgaaagcttc gagcacctga gttaccgacg ggatttgttg ctgaactacg gcccgtacgg
    12061 catgcccggg ttgttgctca aagtggtgcc caatttcacc ttgagcctga cggactacaa
    12121 gggcggccca ccgaccctaa tcaccacgga catcgctcac gccggggagc ggctggtcga
    12181 tttcggtagc ccgaccttca acctgatttc tcgctggatg gagaacgggg cttccgaaaa
    12241 taatgcccct caacccgaga ggaacgccga gcaacggccc tgcaacaccg cgctcggctc
    12301 gggctccgag ttcgatgcca gcgtcgaccc gagtagcgcc gattttcaga tcttccgcga
    12361 ccaagtcaac cctgtgctcg gcgagagctg cgcggccggc aattgccacg gttcgcgcag
    12421 caacacgctc tacctcacgt gcggcagtag cccggagcag gtgcgctgga actactttgc
    12481 ggcttcggac ttcacctctc gagatccggg ctcgagtgac atcgcgcggc gcgcgctggc
    12541 gccctccgcg gggggcactt accacgaggg aggtacggta ttcccgtcca ccgccgatct
    12601 gggctaccag gtgatcctca agtgggcaga gcagaagggc ggccccactc gcatcccgag
    12661 cgagccgggg ttcgagttct tcgcctcgcg cgtgcaaccc atgctcgtga agcggggttg
    12721 tatgatgctg gggtgtcact cggcggccat gttccacgac tatcgcctgc gcggcggcag
    12781 cggtggtcat ttcgggttac cggcgacgcg cctgaactac gagctctctt tgcttcagct
    12841 ggcgctcgag tcacccaacc ccaacgcgag ccgcctcatc gccaagaatc taccgcccga
    12901 gcgcggcggc atcttgcatc gtggcggagc gctgttcgcg agggaagggg tcgcacaggc
    12961 ctgcgatgca acgcgcgccg aatccgggcc actcgatgag caaactccgt actgcgtgat
    13021 cagcgcttgg atcgcaaagg aacgcgccgc gcgtatggcc agcgcggttc cgttcagcgg
    13081 gatcgtgtac gtcaagcgcg ccgcgagccc aggccccgac acccctcagg attgggagcg
    13141 ctcgacggtg gcgggggacg tggtgcgcgt ggcggccacg ctcggggccg atggcacggt
    13201 gacgttgggc gcggaaacga gcctgtcccg ccagtgcggg ctcgatccgg cgacgagcca
    13261 ggcccgccgc cccgccgtat cctgggacgg gcagcgcatc gcattctcgg cgcgcagtgg
    13321 cgccaccgct gcatatcgca tctacgtggt cgacggcacg aactgcgagg ttgaagccgc
    13381 aatcgatcgc gcgcccgtgg actccagtgg cgcgccagtg gcgctgaatg gagagctgtc
    13441 tcacaactta gaccctgcct tcgcgccgga tggtcgaatc gtgttcgtgt ctacgcgcgg
    13501 caacctgcag agcgcgcgcc cgacgggccc acgtcgcacg cccgccgacc cttcgaagct
    13561 ggacacgaac ctctacgtgc gcgagtccga cggtcgcatt cggcaactca cgtatctgtt
    13621 gaatcaagag atgctgccgt ccttcatgcg cgatgggcgc gtgatcttga cgacggaaaa
    13681 gcgcacgctc gggttctatc aactggcggg gcgccgcatc aacctagacg ggggcgacta
    13741 tcatccgctg tttgcccagc gcaagagcat cggctttcag cagttcacct cggtggtcga
    13801 actgcccaac aagaacctgg tggccattgc cagtgagcgc ggtgcagccc acggagccgg
    13861 cgccttggcg ctcgtgaacc gcagcatcgg tatagatcag gggagctccg atccgaagga
    13921 ctacttggtg gatccgagcg cgattacgtt cccgaatcca gacttctttc agcactcgct
    13981 cagcttcatc gatcccgcag ctaccgggca gctctcgggc acgcgcggcg catacttcgc
    14041 gccttccgcc ttcgtggacg ggagaattct cgtgagccat gcgccggatg taacgaacct
    14101 cggggagttc agtggcaact tcgatgtcgt gttggtcgac ccgagtcggc ccgcggcggc
    14161 gccggtcccg ctcatcaccg gtcccgaaga cgcgctctgg ccggtcgcga tttacgcgcg
    14221 ccagaacgcg gggattttct cgtcgcgtct cgacgaggcg aacggcgcga gcgaaatcca
    14281 caccgagctc ggtgaccgcg ccgagattac gtacatggac ttgccgttgc tcggctcgct
    14341 tctatttcaa aacacgcgtt ccgggcgtcg catcagcgaa ggcccgcaga cgtttcaatt
    14401 gtgggagagc ttaccgccgg agccgggggt caccagcttc gctaacgcag gcgcgttcgt
    14461 gacccgtgat caagatggag aggtctacgt gcggcgtggg ctgctcgggg cgctgcagcc
    14521 ttacgcagac ggctccgcca aggtctcctt accgggcggc gtcccgttcg tcgtcgcggg
    14581 ccaattccaa ttggcggagg atgcggcacc gacgcagcgc tttcagcgcg aagagatgca
    14641 ggtttatccg ggcgagcgcg gccgccagtc attccagcgg cgtttcttcg agggcatgtg
    14701 cggtggatgc cacggctcgg ttagcgggca ggagtaccaa gtcgccgtca accccgacat
    14761 cttgactcag gcctcggagg tggccgccaa agacgcgcct gcgatcgtcg tcgagcaaac
    14821 cggcaacgta ctcggccctg aattcccgta atcagaggct ggattgccgc cgctactcga
    14881 ttgcgatcgt cttgctgacc gtgatcttgt cgccatcgaa cgcaggaacc tgagccctgc
    14941 gtaacgtgga agcaatgcag ccaccagttg ccgtgccggc gaaggggggg ccggagaggg
    15001 tcgcgctcgt caccctgccc gatggagcaa acgtgaccgt gacggtggcc acgccggacg
    15061 gatcgccggg cttgcggcaa tgacccgcct gagccgcagc gccctgcaat gcgctcgcgg
    15121 cggcggctcg atcgaacggc cccttgggga tagcaggggc aggaggctcg gccggctctg
    15181 cctcgacggg ttcggaggag ggggcgggct cgcgtttgga gccgagacca gtacgcgccg
    15241 actctgctcg tgcggcgtcc gcgcggggtt cattgcgagc gatcacccgt gcttcggcct
    15301 gagtcagagc gcccgttaca cccgcgccgg gcgtggaccg aagcgacgag cttcgagctg
    15361 cgccattggg agtcgtctcc gtattcctgt tcgcggacga gcgacgagag cttgcatccg
    15421 cgctcgagga tttcgatgcg tccacatccg cagcggagct gtcgccctct cggttgcccg
    15481 cgagtggagc tgcagcttcg ggtctcgttg caggcgtgct gggcgtcggc tcgttgctcg
    15541 tatgttggtc ggtttgcaac gctttcgcga tgactgtttc cgcacccgcg gattgcccgg
    15601 agctcagcgt tttgcgggac gagaccacgg ctgccgtaat cgccaccgcg agcaccaccg
    15661 cgacgcccca ccgcattgaa cggcgttgcg gcccatgctg agtgctttcg tccgccgcac
    15721 ccgagccgac tccttccaaa cgcccactgc ccaccggaag cccgcgtcgg acactcgcgg
    15781 gcgccgtgtg accggagctc gtgtcactag ccgaagccaa aacacgagcc gagcctcccg
    15841 gcactgcttc tgatgcgggg cgctcatgtc ctatggccgc ttgcgacgtt gagctagcaa
    15901 cgggaacgtc gttggcgggt ttgctcgaat cgaatgggtt gtgaaatgcc gtgcgcggag
    15961 tgcgtggcga gccttcgcta tccggaggtg gcgtggagaa ccgaggtagc gtgctcggat
    16021 cgaagctggc cccgagcccc gcgccaccga agccgttgct gccgatgttg aggagacgat
    16081 cggagggccg cggcaccgac tctggcgccg gctcgatcgg ccgcgtcagc gccgcgagcg
    16141 gcggggcgga cgaggctggc ggttctgact gaagtccctc gctgagatcg acatactccc
    16201 ccgctgtcag cggcggcagt gaatcaatcg cgaacaaatc gtggtggttc gtcgcgcccg
    16261 ttgcgctgtc cgccgggttg agcgagcccc gggcaaccgg tgggggtggt ggggaggcct
    16321 cgcccgactt ctcgagcgcg cgggccggag gtttgggcgc gcctttctgc gcgggaacct
    16381 cgtaggcggg cgccgccacc gggatctttg gattggtggg cggatccgtc gcgtcaacgc
    16441 ggatgggcgc gttgcaacgc tggcaacgca gggtgacgac cttgcccacg aacttgcgac
    16501 ggtacagctc atcgctgacg ttgaaacgtg cgccacatcg attgcagttc accgcgtaag
    16561 gcacgctcag tccgctcctc aagctggggt cgcttgccac gacccaacgc ccgccgagta
    16621 ttccttcaat gacccggcat cgcaagcacc gtgcgttggc cgggatgcct gaccgtcaag
    16681 tttggcgccc tcgtgcaacg atcgccgaga gatgtcgccc gctttgcgcc ccgtcgcggc
    16741 gaaacgagaa gaattgccgc gcatccgaaa cggtgcagcc atcgacatcg tcaatccagg
    16801 acgcttccac gccgagggcc aggagcttgg cccgcacgat gcgtcgcaga tccacgtggg
    16861 gtcgtgcgcg gctccggtcg acgacccctg ggtcggggga ggcttgctgt aattggtgcg
    16921 ccacttcgtc ggagacttcg aacgcagctc gcgaaatgtg tggaccgatg gctgctaccc
    16981 actcgggtgc ttctgattgg ctccgcgcta gttcccgcag cgcgcattcg atcaccgagc
    17041 ccaccacccc acgccagccc gcgtgaacgg cggccacggc cccgctgcga cgatctgcaa
    17101 taaggatcgg aacacagtcc gcggtgcgca ccgcgcaggc caagccggtg ctcgcgctca
    17161 ggatg
//
LOCUS       DASTKU010000626         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1460528, whole genome shotgun sequence.
ACCESSION   DASTKU010000626 DASTKU010000000
VERSION     DASTKU010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460528"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1995)
                     /locus_tag="VFQ61_21940"
     CDS             complement(52..1995)
                     /locus_tag="VFQ61_21940"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9957181.1"
                     /translation="MKLNRGMCFRALGWGWLASMASAGIGGLGACSSASSSPGALAGQ
                     TSSAGGQTSVSGGSTSGGNTNPGGSGPGGAAAGGNATGGVGAGGAPTGGKTGTAGAAP
                     ATGGSMNQGGSAGSAGMPQTAKDTCAPAPSGAVTFSVPGGTFQGTLNVELHTEAGAEI
                     RYTLDHTPPSATSPLYAGPIAITATTDLRAAAFANGVAVGKASAAVYVARSFDATHDL
                     PVMVLDSYGRALPAQPMGGFGIPAPVPRDYMDAATLTYELKNGAATLSTAPQVASPGA
                     FHIRGQSSASYAKKPYRLEMREADSTDRNCPMFGMPRESDWVLHAPFPDKALIRNAFT
                     YGLGREMGLPAPRFAFAELYLNTATRPLEAADYQGVYLLVETIKNQKDRLNLQQLKEQ
                     DTMMPAVTGGYIFKFEWQVTDIEQQLTCPAEQANCWNWLEVIDPKPWVPEQQAYLTSY
                     LRQFVDALHSANPSDPTTGYPAFLDVRSTVDQVIINEFTRNMDAYARSQFFYKDRGGK
                     LFAGPLWDYDLIAGVGISMTYPNLDTAGFQYESNATRLAQTVDWFPLLLAEPAFQAAL
                     VARWKELRQTLLSDAAIGQRITALTQGLSGAAQRNFQKWNNLTTARIGFFETPVVDTW
                     QGQVDVMRTWLLQRAAWLDTAWK"
     gene            complement(2318..>3741)
                     /locus_tag="VFQ61_21945"
     CDS             complement(2318..>3741)
                     /locus_tag="VFQ61_21945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957182.1"
                     /translation="KAVKSRASSASRSRGTPVWPHLLGAVLGISSGSVTQAAAQPRTE
                     PPLLFGGARPRGRPEIPIDPSLPTGTRVRPAERPRELGLRVCSWRYPVCVHGQEASSP
                     RLLNSALNALEAAYERVIVALGLPPPLPDWGRGGDDGVDAYLTAPDRPLQVFDDEPWA
                     GPFARSSAFCSIPEVTSDAHWEWARSATLCLAEASSIALDASETPHLKRAFATWVWWL
                     SGLPTSLDVQAIHDVQRTPELAIATRELTPFSEGAAIFFEYLETVRSQSEPLALSTAL
                     FSAAASPTPSSADYVNEPDLFDVLRHTLDEQFAKFGALMADFAVARALLGDDSHFPNL
                     AFAGDFGRMRFDWSLDFSSLPRRVLVKPALESTGVVAIRLRLDDVALGQALGFRAEWE
                     APVSFQWQLVKLNAEGKEMGRIDVPFQERAREAEARVSWLDGVASILIVGTNLEGIDL
                     SHPFDPDVSPFEPHSATVYLSRL"
BASE COUNT          738 a         1162 c         1155 g          686 t
ORIGIN      
        1 cccgcagagt gacagggagc gcgttcttct acctctgatt aaggggaccg cctacttcca
       61 agccgtgtcg agccaagcgg cgcgctggag caaccaagtg cgcatgacgt ccacctgtcc
      121 ttgccaggta tccaccactg gcgtctcgaa gaagccaatc cgagccgtgg tcaggttatt
      181 ccacttctga aaattgcgct gcgcggcccc gctcaggccc tgggtgagag cggtaatgcg
      241 ttgcccaatg gcggcgtcgg acaacagcgt ttgtcggagc tctttccagc gtgccacgag
      301 cgcagcttgg aaggctggct cagccagtag cagcgggaac caatccacgg tttgcgccag
      361 gcgcgtcgcg ttgctctcgt attggaagcc cgcggtatcg agattgggat aggtcatgct
      421 aatccccacc cccgcgatca ggtcgtaatc ccagagtggt cccgcaaaca gcttgccgcc
      481 gcgatctttg tagaagaact ggctgcgcgc ataagcgtcc atgttgcgcg tgaactcatt
      541 gataatcacc tgatcgacgg tggagcgcac gtccaggaac gccgggtagc ccgtcgtggg
      601 atcactcgga ttggcggagt ggagagcatc cacgaattgc cgcagatagc tagtcagata
      661 ggcttgttgc tcgggcaccc acggcttggg gtcgatgact tcgagccaat tccagcaatt
      721 ggcctgttcg gccggacacg tgagttgctg ttcgatgtcc gtaacctgcc actcgaattt
      781 gaagatgtag ccgccagtca cggctggcat catcgtatct tgctctttga gctgctgcag
      841 attcagacgg tctttttggt ttttgatcgt ctctacaagc aggtatacac cctgataatc
      901 tgctgcttcc agcggccgag tcgcggtatt caagtacaac tcggcaaagg cgaagcgggg
      961 cgcaggcagg cccatttcgc ggcccaagcc gtaggtgaag gcattgcgaa tcagcgcctt
     1021 gtccgggaag ggcgcgtgca acacccagtc cgactctcgc ggcataccga acattgggca
     1081 attacgatcc gtcgagtccg cctcccgcat ttccaagcgg tagggcttct tggcatacga
     1141 tgccgacgac tgaccacgga tgtggaacgc gcccggactc gccacctggg gcgccgtgct
     1201 caatgtcgcg gcgccgttct tcaactcgta agttagagtc gcggcgtcca tgtaatcccg
     1261 cggcaccggc gccgggatcc cgaatccacc catgggttgg gccggcaagg cacgcccgta
     1321 ggagtcgagt accatcacgg gtagatcgtg agtggcgtcg aaactccgcg ccacatacac
     1381 ggctgcggag gccttgccga ccgcaacgcc gttggcgaag gcagccgccc ttagatcagt
     1441 agttgccgtg atcgcgattg gaccggcgta gagtggagaa gtggcgctgg gcggcgtgtg
     1501 gtcgagcgta taacggatct ccgcacccgc ttcggtgtgc agctccacgt tgagagtgcc
     1561 ttgaaaagtg ccgccgggta ccgagaatgt aaccgcgccg ctcggtgccg gtgcacaagt
     1621 gtctttcgcg gtctgtggca taccggcgct accggcactg ccgccctgat tcatgcttcc
     1681 gccagtggcc ggagcagcgc cggcggttcc ggtcttgcct cccgtaggcg cgccacctgc
     1741 gccaactccg cccgtcgcgt ttccgccggc agcagctccg cccggaccag agccgccggg
     1801 gttggtattg ccaccggatg tagatccgcc cgaaacagag gtctgtcctc ccgcactcga
     1861 ggtctgcccg gcgagcgctc ctggagagga actcgcactc gaacaagctc cgagccctcc
     1921 gatgccagcc gacgccatac tagccagcca gccccacccc aacgcgcgaa agcacatacc
     1981 tctattcagt ttcataccaa gaatccgtcc aactcgaagt tcttagctct agtaccccag
     2041 cgcatgtatg gacgtcaggc aattcgacac cgaatcttca caattctgcg cgtcggtgga
     2101 tcgtgggtcg ctgacatagc gcgccaagcg cggtcacacg cgcgagcctg taggctacca
     2161 actccaaggg gcaggaaacg cgcgggatct ggtggttctg agcttcaaat aactcgcagc
     2221 acgcgtttgt atttcgaata atgccgtcga ctgcattttg ttatagctag tgctgcgtcc
     2281 ccgcagttgc gaatcccagg ttcgctgcgg agctccgtca tagtcggctg agatagacgg
     2341 tcgcggagtg cggctcgaag ggagacacat cgggatcgaa agggtgtgag aggtcgattc
     2401 cttctaggtt ggtcccgacg atcagaatcg acgccacacc gtctagccaa ctgacgcgcg
     2461 cctccgcctc gcgagctcgc tcttgaaagg gcacgtcgat gcgacccatc tctttgccct
     2521 ccgcgttcaa cttaacgagt tgccactgga aactcaccgg agcctcccat tcggcacgaa
     2581 agcccagggc ttgaccgagg gccacgtcgt cgagtcgcaa gcgtatcgcc actactccgg
     2641 tggactcgag tgctggtttg acgagcacac gccgcggcag tgacgagaag tcgagagacc
     2701 aatcgaagcg catacgtccg aaatccccgg cgaacgccag attcggaaag tgggaatcgt
     2761 ctcccaacaa cgcgcgggcc accgcgaagt ccgccatcag cgcgccaaac tttgcgaatt
     2821 gttcgtccag agtgtgccgc agcacatcga aaaggtccgg ctcgttgacg tagtccgcac
     2881 tcgatggtgt tggcgacgcc gccgccgaga agagcgccgt ggacaaggcg agcggctcgc
     2941 tttggctacg gacggtctcg aggtattcga agaagattgc cgcgccctca ctgaaagggg
     3001 tcagctcacg agtcgcgatc gcgagttcgg gagtgcgctg cacatcgtgg atggcctgga
     3061 cgtcgagtga agtcggcagg ccactcagcc accaaaccca agtggcgaaa gctcgcttca
     3121 ggtggggagt ctcgctcgcg tccagggcga tgctgctggc ctcggccaag caaagggtcg
     3181 cagagcgagc ccactcccag tgcgcgtcgc tcgtgacctc cggaatggaa cagaaggctg
     3241 acgagcgggc aaagggccca gcccaaggct cgtcgtcgaa tacttggagg gggcgatcag
     3301 gtgcggtgag gtaggcgtca accccatcgt cgccgccgcg tccccaatcg ggcagtggag
     3361 gcggcaggcc gagcgccacg atcaccctct cgtaggccgc ctcgagggcg ttcaacgccg
     3421 agttcaatag acgcggggaa ctcgcctcct gcccgtgcac gcagacgggg taacgccaag
     3481 aacaaacgcg cagccccagc tcacgaggcc gctcggcagg cctcacgcgt gtaccggtgg
     3541 gcaagctcgg atcaatcgga atctcgggtc gtccccgggg ccgtgcgcct ccaaagagca
     3601 aaggaggctc ggtgcgaggc tgagcggcgg cttgcgtaac acttccggaa ctgatcccaa
     3661 gcacggctcc gagcaggtgc ggccacactg gcgtaccgcg cgaccgactg gcggacgaag
     3721 cccgagattt gacagctttc a
//
LOCUS       DASTKU010000627         8402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1237018, whole genome shotgun sequence.
ACCESSION   DASTKU010000627 DASTKU010000000
VERSION     DASTKU010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8402
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237018"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3351)
                     /locus_tag="VFQ61_21950"
     CDS             complement(<1..3351)
                     /locus_tag="VFQ61_21950"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9957183.1"
                     /translation="MNSREQPQSAGTVVQCLARWARTEPNRVALRFLGDGEQVSDTLT
                     FAELDARARKIAGHLQQISRVGERALLLFPSGTGYIDAFFGCLYAGVVAVPAYPPEAA
                     QPQQLKRLWSIVDDCKPRLILTLAVFIEPLRAVLAASGRVLDIELVAVDAVDSGGAQR
                     YRAHAPDANAVAFLQYTSGSTSEPKGVIIGHDNLAANELSIQRGFGIEPNDTIVTWLP
                     LYHDMGLIGGLLQPLFSGANLVLMSPRHFLEKPARWLEAIARFQGSVSGAPDFAYRMC
                     TERVSDAVLDRLDLSSWRVAFSGSEPVREATLQLFAERFSRRGFDAKAFYPCYGLAES
                     ALFVSGGQRGDGARVVEVDKDALARGVAEPGATPVRLVGCGWSDPNHRIIVADLRTGA
                     ALGDNVVGEIWVAGPSLAHGYFGNPEATAETFVEHSGMRYLRTGDLGFTRQKQLFVSG
                     RKKDLIIVRGQNVYPQDVESTLEASVEALRKGRIAAFAVENQGQESIGVAAEIVRGMQ
                     KFIHPEAVAQAVREVVAERHGEAPALVALLAPGALPKTSSGKLQRLATARAIALAELE
                     TVFVWRAEGFKSELPEAPPTGEEVGAPVSARREVIRNIWQRVLGVERVEERDNFFALG
                     GNSVAVVSVIAEVERDVGVRVEPSALWCSATLEAFAAAIEGATAERSQEGEALAASPA
                     QDRAERSTHSPPTLSPLSPSQRRFWLLWKLRPDSAAYHVQAAVGLQGSLGHGSLGQPA
                     TSEARWERALENIVQRHTALRTVFDERGTHQIVRARSAVCFVSYDFATASSSLEQSTA
                     LARAAAEADAREPFDLVTGPLVRVSVGRLGPERHVLWVTAHHVAVDGFSLQLLLEELV
                     EAYFSANGPAGRLEGQPGSDYTKYAAGQLKLLEAPAAFATAIERYRSQRGDGAYVLRL
                     PIDRPRPVSPSQVGASVHFELPAELTRSMHQLARAHELTPFAVLLGAFKVLLYRLSGE
                     MDIRVGVPMSNRTSLDAMRVIGPLVNTAVSRSVLSGAKSFSHLLGELRGSLVMLQRDQ
                     ALPFELLVQALGVRTDPSHTPLFQVLYNHQVLRWSELEQRCGLELELLPWEPPTAPFD
                     LVLDTYEDDQGRCFGRFGYARELFEETTI"
     gene            complement(3451..4422)
                     /locus_tag="VFQ61_21955"
     CDS             complement(3451..4422)
                     /locus_tag="VFQ61_21955"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HET9957184.1"
                     /translation="MMDMAKDTEMSCGAPERIPVVLSGDGRGNSLLDADLRERIEQVL
                     EGEGAVLLSGYERLGVDGFRAFAQRFGHPLVDYEFGSTPRTEVSAGIYTSTEYPAHQS
                     IPLHNEQSYALTWPMKIWFYCDVAPSHGGETPIADSRAVYRRLKPELRRRFEDKRLMY
                     VRNFGGGLDVSWQMAFGTDSRAAVEAYCRNNAIVCEWKSDGELRTRQVCQASARHPRT
                     GQRVWFNQAHLFHITALAPDTREALLDCVDQEDLPRNVYYGDGSAIEDPVLDDVRAAW
                     EAEKRKFRWSPGDVLMLDNMLCAHGREPFSGPRRIVVAMAEPYSQGR"
     gene            complement(4406..5251)
                     /locus_tag="VFQ61_21960"
     CDS             complement(4406..5251)
                     /locus_tag="VFQ61_21960"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9957185.1"
                     /translation="MNTRVLAPPVTLARLICLPHAGASASVYGKWQALAPSWFEISGL
                     ELPGRGRRASEAPARDLGALSEQLAREIALESRSLVGQSSSPHVRHSLARCPYALFGH
                     SMGAVLAFEIVDRLRAFGAEGPIALFVSGREAPDLRRPTRYRGLQGDAELVAELRRLG
                     GTPELIFQNRDLLEMTLAVLRADFAACANYRYRERPPYGCPLHALGGLQDRATPSELG
                     EWARQTSSTFTLTMFEGGHFFHHRHTARVVSMIAERVREALPENPVRFSAMDLDRESS
                     NDGHG"
     gene            complement(5248..5520)
                     /locus_tag="VFQ61_21965"
     CDS             complement(5248..5520)
                     /locus_tag="VFQ61_21965"
                     /inference="COORDINATES: protein motif:HMM:NF015578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="HET9957186.1"
                     /translation="MSFDSETIVFVVVVNHEEQYSIWPEYKPIPNGWRDAGKRGVKKE
                     CLEYIDRVWTDMRPLSLRRQMDGDAQTSGGHSKTDDNQPTGGDVLP"
     gene            complement(5604..8024)
                     /locus_tag="VFQ61_21970"
     CDS             complement(5604..8024)
                     /locus_tag="VFQ61_21970"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET9957187.1"
                     /translation="MRRRRSGTGVQALKLRALILSLSAFALVPVCGYWWLCRSRPELS
                     GSSQAHGLKHTAAVDTDQEGLVTVRAASAEDAYVALGFAHARDRLWQMELTRRAARGT
                     LSEAFGAKLVRSDRWMRTLGFTEVCEQNFRVLSLEARTVLEKYAEGVNAFLKLGRPLP
                     PEFALFSVTPEAWSSLDSLLTLKLMAWSLSGNFWDELVNVRLLAALPLARALDLAPVP
                     TGEPERIIEALDGDEIGELEPVARALFDAAPAGAAPGVGSNAWVVDGLHTQSGKPLLA
                     SDPHLMLSAPAVWYLARLESPEVEVAGATIPGLPTVVLGHNGHVAWGYTNTRPDTQDL
                     FIERLDAERPDHYVTPEGSAPFVSRMETIHIKGEPDLKFEVRATRHGPVISDADDGVR
                     RAMPKGRVLALAWVTLRDDDQSFEFSVKAARARRAAELLEAARFFHSPQESLVFADIE
                     GQVGYVVAGRVPLRAPSNIVKGRLPAAGWQRDSDWQGFIPFEALPQGLGAPSGKIVTA
                     NQRVTPANYPFWMTANWASAERAHRISALLERAPSSHTLETFAAIQLDDREPGVLDLV
                     AHFTRMRAMTSEQAHLITALRAWDGSMAPDRLEPLLFAEWSRRFTLALYARKLRDLSD
                     ILDEYHPEFLRRVFAVPAATEYWCAPGQPVPCEAELRSALQEAQLWIVSHYGSEPKSW
                     SWGKANMARFAHRSLGRIPLLSKLFSVEVPRGGGYESVNVSGYFFDRAADGYVTESGP
                     GFRALYDLSDLSRSRFMLGPGQAGHPLSPFYSNLAQPWASGGYVVFGRSRAQSWVLEP
                     EPRSLAVP"
     gene            8141..>8402
                     /locus_tag="VFQ61_21975"
     CDS             8141..>8402
                     /locus_tag="VFQ61_21975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957188.1"
                     /translation="MKLVYWLVWQHRVWLLGVLALSGLSASISVGVIALIHELIATDS
                     VLRRVILARFCGLLLALFVTGVASQLIVSWLGHGAVYRLRRVL"
BASE COUNT         1662 a         2782 c         2443 g         1515 t
ORIGIN      
        1 gatcgtagtc tcttcgaaca actcccgcgc gtaaccaaag cgaccaaagc atcgtccttg
       61 gtcatcctcg taagtatcca atacgagatc gaagggggca gtgggtggct cccaaggtag
      121 caactcgagc tcgagcccgc atcgctgctc gagctcggac cagcgcaaca cttggtgatt
      181 gtataggact tggaacaacg gtgtgtggct cgggtcggtg cgcacgccga gcgcctgcac
      241 tagaagctcg aacggcagcg cttggtctcg ctgaagcatc accaacgagc cgcgcaactc
      301 gccgagcagg tgactgaaag atttcgcccc gctgagtacg ctgcgcgata cggccgtgtt
      361 caccagtggg ccaatgaccc gcatggcatc cagactcgtg cggttgctca tgggcacgcc
      421 gacacggatg tccatctcac cgctgagccg atacaagagc actttgaacg cgcccagcaa
      481 caccgcaaat ggggtcagct catgcgcacg cgcgagctga tgcatgctcc gagtcagctc
      541 ggcgggcaac tcgaagtgaa cgctcgcgcc aacttggctc ggcgaaaccg ggcgaggacg
      601 atcgattggc aagcgcagga cgtacgcgcc atctccccgc tggctacggt agcgctcaat
      661 cgccgtggcg aaggcagcgg gggcctcgag aagcttcagc tgccctgcgg cgtacttcgt
      721 gtagtcggat ccgggttgcc cctcgagtcg accggcgggg ccgttcgccg agaagtaagc
      781 ttcgaccagc tcttcgagca ggagctgcag cgagaatccg tccacggcaa cgtggtgcgc
      841 ggtgacccac aacacgtgtc gttccgggcc cagccgtccc acggaaacgc ggaccagtgg
      901 ccccgtcacc agatcgaagg gctctcgcgc gtcggcttca gcggccgccc gtgccagagc
      961 cgtggactgc tccagcgacg aactggcggt cgcgaaatcg tagctcacaa agcacaccgc
     1021 gctgcgggcg cgcacgatct ggtgtgtgcc tcgttcatcg aacaccgtac gcagcgccgt
     1081 gtgacgctga acgatattct cgagagctcg ctcccagcgc gcctcggatg ttgcgggctg
     1141 acctagacta ccgtggccca agctaccctg gagaccgacc gccgcctgaa catgataggc
     1201 cgcgctgtcg ggccgcaact tccaaagcaa ccagaagcgg cgttgggatg gagagagtgg
     1261 agagagcgtg gggggggagt gagttgaccg ttctgcgcga tcctgggcgg gcgatgccgc
     1321 cagcgcctcg ccctcctggc tccgctcggc ggtggccccc tcgatggcgg ccgcgaaagc
     1381 ctcgagggtt gcagaacacc aaagcgcgct cggttccaca cgcaccccga catcgcgctc
     1441 gacttcggca atgactgaca cgaccgccac cgaattgccc cccagagcaa agaagttatc
     1501 tcgttcctcg acgcgctcga ccccgagcac ccgctgccag atattccgaa tcacttcccg
     1561 tcgagcgctc accggggcgc ctacttcctc gccagtagga ggagcttccg gaagctccga
     1621 tttgaagccc tctgccctcc agacaaagac tgtttctagc tccgcaagcg cgatcgcccg
     1681 cgccgtggcc aatcgttgca gcttgccact cgaggtcttc ggcagcgctc ctggtgcgag
     1741 caacgctacg agcgcagggg cctcaccgtg ccgctcggcc accacttcac gcacggcctg
     1801 agccacagct tcggggtgta tgaacttctg catgccgcgc acgatttccg ctgcgactcc
     1861 gatgctttcc tgaccttgat tctcgaccgc gaaggcggcg atacgaccct ttctcagcgc
     1921 ctccacgctt gcctccagcg tcgactccac gtcttgcgga tagacgtttt gcccgcgcac
     1981 tatgatcagg tccttcttgc gcccgctcac gaacagctgt ttttgacgcg tgaacccgag
     2041 atccccagtg cgtagatacc gcattcccga gtgctccacg aaggtctccg ctgtcgcctc
     2101 tgggttcccg aagtagccgt gggcgaggct cggtcctgcc acccagatct ctccaaccac
     2161 gttgtcgccc agagctgctc ccgtccgtag atcggcaact atgattcgat gattgggatc
     2221 cgaccatccg caacccacca ggcggaccgg agtggcgccc ggctcagcca cccctcgcgc
     2281 cagcgcgtct ttgtcgacct ccactacccg cgctccgtca ccgcgctgcc cgccagagac
     2341 gaatagcgcg gactcggcca gcccgtagca agggtagaat gctttggcat cgaagccgcg
     2401 gcgtgagaac cgctccgcga agagctggag cgtggcctcg cgaaccggtt cggagcccga
     2461 gaaggccacg cgccaggagc tcaaatccag ccggtccagc accgcatccg agacgcgctc
     2521 cgtgcacatc cgatacgcaa agtcgggcgc gccgctcact gagccttgga aacgcgcaat
     2581 cgcctcgagc cagcgagcgg gcttctccag gaaatgccgg ggcgacatca acaccaagtt
     2641 ggcaccggaa aacaacggct gcagcagccc gccaatcaaa cccatgtcgt ggtaaagcgg
     2701 caaccacgtc acgattgtgt cattgggctc gattccgaat ccgcgctgga tactgagctc
     2761 gttcgcagcg agattgtcgt ggccaatgat gactcccttc ggctcggagg tcgagccaga
     2821 cgtgtactgg agaaacgcga ctgcgttcgc gtcgggagcg tgagctcggt agcgctgcgc
     2881 gccgcccgag tctaccgcgt cgacagccac taactcgata tcgaggaccc gccccgaggc
     2941 ggccagcacc gcgcgcagag gctcgatgaa aaccgcgagg gtcaggatca gccgaggctt
     3001 acagtcgtcg acgattgacc acaaacgctt gagctgctgc ggttgagccg cttcgggcgg
     3061 ataggcggga acggcgacga ctccagcata caagcaaccg aagaaggcat cgatgtagcc
     3121 agttccggat gggaacaaca gtaacgcgcg ttcaccgacc cgcgatatct gctgaaggtg
     3181 ccctgcgatt ttgcgtgcac gcgcgtcgag ctccgcgaag gtcagcgtgt cactgacctg
     3241 ctcgccatcc cccaagaagc gcagcgcgac tcgattgggc tccgtgcgcg cccagcgcgc
     3301 caagcattgg acgacagttc ctgcagactg cggctgttct cggctgttca ttctctagtc
     3361 ctttgaactc cacacgcgac ccaaaacgat accgacgcac gcgccgaagg cgcgttccca
     3421 attcgtaagt gacaagtggg gaagctgcgt tcagcgcccc tgcgaatacg gctccgccat
     3481 cgccacgaca atgcgccgcg gccccgagaa cggctcacgc ccgtgtgcac acagcatatt
     3541 gtccagcatc aacacatcac ccggactcca cctaaacttg cgtttttccg cttcccaagc
     3601 tgcccgcacg tcatccaaga ccgggtcctc tatggcggaa ccatccccat agtaaacatt
     3661 ccgaggcaga tcttcttggt cgacgcaatc gagcaacgcc tcgcgagtat caggggcgag
     3721 tgcggtgatg tggaataggt gagcttgatt gaaccagacc ctctgcccgg tacgcgggtg
     3781 gcgagccgag gcctggcaca cctgccgcgt gcgtagttca ccgtcagact tccactcgca
     3841 aacgatggca ttgtttcggc aatacgcctc gaccgcagcg cgagaatccg tgccgaatgc
     3901 catttgccac gacacgtcca gtcctccgcc gaagttgcga acgtacatca atcgcttgtc
     3961 ctcgaaccgc cgcctcagct cgggctttag ccgccggtat accgcgcgac tatccgcaat
     4021 cggtgtctct ccgccgtggc tgggagccac gtcgcaatag aaccaaatct tcatcggcca
     4081 ggtgagggcg tacgattgct cgttgtggag cgggatgctc tgatgcgctg gatattcggt
     4141 ggatgtgtag atccccgccg aaacctccgt gcgaggcgtg gagccaaact cgtagtcgac
     4201 gagcgggtga ccaaaccttt gggcaaacgc ccggaatccg tcgacgccca gccgctcgta
     4261 tcctgagagc aacaccgccc cctcgccttc cagcacctgc tcgatgcgtt cacgaagatc
     4321 cgcgtccaac agggagttgc cgcgaccatc gccgctcaat acgaccggaa tccgttcggg
     4381 cgccccgcag ctcatctccg tgtctttagc catgtccatc attcgagctc tcgcgatcta
     4441 gatccatggc actgaagcgc accgggttct ccggtagagc ctcacgcacg cgttccgcga
     4501 tcatactcac gactctcgcg gtgtgccgat ggtgaaagaa gtggccgccc tcgaacatcg
     4561 ttagcgtgaa ggtactactg gtctgacgtg cccattcccc gagttcggac ggagtggcgc
     4621 gatcctggag accgccgaga gcgtgcaaag ggcagccgta gggcggccgc tcgcgatatc
     4681 gatagttcgc gcaagccgcg aagtccgctc ttagcacagc aagggtcatc tccagcaaat
     4741 cacggttctg aaaaatcagc tccggagttc ccccgagcct cctcaactct gcgaccagct
     4801 cggcatcccc ctgcagccca cgataccgcg taggtcggcg gagatccggc gcttcacggc
     4861 cagaaacgaa tagagcgatc ggaccttccg cgccgaaagc tctcagccga tccacgattt
     4921 cgaaagcaag gaccgcaccc atgctgtgcc cgaacagggc gtaaggacaa cgggccagcg
     4981 agtggcgcac gtggggagag ctggactgcc caacgagcga cctgctctcc agggcaatct
     5041 cacgcgcgag ctgctcggaa agcgcgccaa gatcccgagc cggtgcctcg ctcgcacgac
     5101 ggccccgccc gggcaactcg aggcccgaga tctcgaacca actcggcgcc aacgcttgcc
     5161 acttcccgta gacggacgca gaggccccgg cgtgaggcaa gcagatcaaa cgagcgagcg
     5221 taactggtgg tgcgagaact cgagtgttca cggcaacacg tcgccgcccg tgggctgatt
     5281 gtcatctgtc ttactgtggc caccactcgt ctgcgcgtct ccatccatct gccgtcgcag
     5341 gctcagcggc ctcatatcgg tccagactcg atcgatgtac tccaaacatt ccttctttac
     5401 gccgcgcttg cccgcatcac gccatccgtt tggaataggc ttgtactcgg gccatatcga
     5461 atattgctct tcgtggttga ctaccaccac gaatacgatc gtttcgctat cgaagctcat
     5521 ttacatctcc tcgattcaat cggattctca gttcaattca gtcgtcgaat ctcgttggtc
     5581 cacgccagaa acggagaccc tagtcaaggc accgcaaggc tcctcggctc gggctcgagc
     5641 acccagcttt gagctctgct tcgaccgaaa actacgtatc ctccgctcgc ccagggctgc
     5701 gcgagattgc tgtagaatgg agaaagcgga tgtcccgctt gccccggccc gagcatgaat
     5761 cgcgagcgcg acaagtcgga gaggtcgtag agagctcgaa atcccggacc tgattcggtc
     5821 acgtagccat ccgccgcacg gtcgaagaaa tagccactca cgttcacgct ttcgtaaccg
     5881 ccaccgcgtg ggacctcgac gctgaacagc ttggacagga gcgggatgcg cccgagcgat
     5941 cggtgggcga agcgagccat gttggcctta ccccaagacc aactttttgg ttccgagcca
     6001 tagtgactca cgatccagag ctgcgcttcc tgcaacgcgc tccgcagctc tgcttcacaa
     6061 ggaaccggct ggcccggggc gcaccagtat tcagtggctg caggcaccgc gaacactcgg
     6121 cgcaaaaatt cgggatggta ctcatccagt atatcgctca gatcccgcag ctttcgcgca
     6181 tacagggcca acgtgaaccg ccgtgaccac tccgcgaaca gcagcggctc gagacgatcc
     6241 ggcgccatcg atccgtccca ggcgcgcagc gccgtgatca gatgggcttg ctcggaggtc
     6301 atggctcgca tgcgggtgaa gtgcgccacc aagtccaaaa ccccgggctc gcgatcgtcg
     6361 agctggatgg ccgcgaaggt ctcgagtgta tgagagctcg gagcgcgctc caggagcgcc
     6421 gatattcggt gcgcgcgctc cgccgacgcc caattcgcag tcatccagaa tgggtagttc
     6481 gccggcgtaa ccctttgatt ggcagtcacg atcttgccgg atggcgcgcc tagaccctga
     6541 gggagagcct cgaatgggat gaacccctgc cagtcactgt ctctctgcca gccggccgcc
     6601 gggagtcggc ctttcacgat attggacggc gcgcgcagtg gaacacgacc cgcgacgaca
     6661 tagcccacct gcccctcgat atcggcgaat accaagctct cttggggtga atgaaagaat
     6721 ctcgcggcct cgagtaactc ggccgctctg cgggcacgag cagcttttac cgagaactcg
     6781 aagctttggt cgtcgtcgcg aagggttacc catgccaggg cgagtacacg acccttgggc
     6841 attgcgcgcc gcacgccgtc gtccgcgtcg gagatcacgg gcccgtgtcg ggttgcacgc
     6901 acctcgaatt tcaggtcggg ctcgcccttg atgtggatcg tctccatacg ggacacgaag
     6961 ggcgcgctcc cctcgggcgt cacgtagtga tcgggtcgct ctgcgtctaa tctctctatg
     7021 aataagtcct gcgtatctgg acgggtgttg gtgtatcccc atgccacatg gccattgtgg
     7081 cccaacacga ctgtaggcaa ccccggtata gtcgcgcccg cgacctcgac ctctggcgac
     7141 tccaaccgcg ccaagtacca gactgcgggg gcactcagca ttagatgcgg atcgctcgca
     7201 agaagtggct ttccactttg cgtatgcaac ccgtcgacga cccaggcatt cgagcctacc
     7261 cctggtgccg caccggcggg cgccgcatcg aacagagctc gcgccactgg ctcgagttct
     7321 ccaatctcat ccccatcaag cgcctcgatg attcgttccg gttcgcctgt cggcaccggc
     7381 gccaagtcta gggctcgcgc aaggggaagc gccgccaaca atcggacatt cacgagctcg
     7441 tcccagaagt tgccggacaa actccacgcc atcagcttta gagtcagaag cgaatccagt
     7501 gatgaccaag cctcgggtgt caccgagaac agagcgaact ccggtggcag cggccgtccc
     7561 aacttcaaga aggcgttcac accctccgcg tacttctcga gaaccgttcg tgcctccagg
     7621 ctcaaaacgc ggaagttttg ctcgcacact tcggtaaatc ccagcgtcct catccagcga
     7681 tcggaccgca ctagtttggc tccaaacgcc tcggagagcg tgccgcgcgc ggcgcggcga
     7741 gtgagctcca tctgccaaag tcggtcccgt gcgtgggcga agcccagcgc cacgtaagca
     7801 tcctccgcgc tcgcggcccg caccgtcacg agcccttcct gatcggtatc taccgccgcc
     7861 gtatgcttga gcccatgcgc ttgcgagctg cccgacaact ccggccgtga tcggcagagc
     7921 caccagtaac cgcagacggg cacgagcgcg aaagcactga gcgacagaat gagagcgcgt
     7981 agcttcaatg cttggactcc cgtcccggag cgtcggcgcc gcatgcgccc acagcgaaga
     8041 tacgaatgat tcgcattttc ttgatcggca tgctactcag cgaacctccg atgtcaacga
     8101 ctatcgagcc cgttcacaaa cccgcagagg agctccgcgc atgaagctag tgtactggct
     8161 agtttggcaa catcgtgtct ggctgctcgg cgtgctcgcg ctcagcgggt tgagcgccag
     8221 catcagtgta ggagtgatcg cgctgattca cgaactgatc gcgaccgaca gcgttctgcg
     8281 cagagtaata ctcgcccgat tctgcggtct gctcctggct ttgttcgtga ccggagtggc
     8341 atcgcagctc atcgtttcat ggcttggcca cggggcagtc tatcgattgc gccgagtctt
     8401 gg
//
LOCUS       DASTKU010000628         4970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1012767, whole genome shotgun sequence.
ACCESSION   DASTKU010000628 DASTKU010000000
VERSION     DASTKU010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012767"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VFQ61_21980"
     CDS             complement(<1..787)
                     /locus_tag="VFQ61_21980"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957189.1"
                     /translation="METTIGKYRLGRVLGSGGMGRVFAAHDTVLEREVALKRMHGTAQ
                     ASPESRERFAREARAIAALRHPNVVQIYDHGLAEDGSLYTVMELLEGEDLGHRLGRVT
                     SLTPGAFAPILVQVAAALDAAHSARIVHRDLKPENIFITSHGTESVVKILDFGVASIQ
                     GQLATKDSHLTRQNQLLGTPAYMSPEQAEGLAVDHRSDLWSLGAIAYQALTGRLPFRG
                     ENAVATLIRICTREPEPPTSLVASLPRELDRFFARALSKDPGGR"
     gene            complement(1134..>4970)
                     /locus_tag="VFQ61_21985"
     CDS             complement(1134..>4970)
                     /locus_tag="VFQ61_21985"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9957190.1"
                     /translation="TVTRIEVPPIAPFDVEELLADALSMPRDAVRDLASLVHARTGGN
                     PFFLRIFVKTLVEDGALRFGPNGWAWSMTELRERGVTENIAKVLSNQIRRLPNRTHTL
                     LELAACLGSQFPLETLRRVEPRLDLETDLWPAIDAGYLIPAGYIHSPADLSGPNLESS
                     LRFAHDRVREAILGRLPPPQREALHRQIASALANHPQAAEGPMLFELVGQLNQSGDPP
                     SPDERLERARLNLLAATRSIAASSAQRALSYATTGIDLLRQGGNAEPLRHELQTTACE
                     AAFSCGKFDTFTDLAQQALSQSLAPLEEAKILGLAGHVAYARGDGLRALETYLRALTS
                     LSFWPAEPLGSQAEVVAICALSDERILSLPACTDARALLGMELLDPAILTAFYAGSAE
                     LPALLNLCCGLTLRFGLTPYSAFAITILGVLELGQNRFESAMRLAQLALAIAERFGND
                     EISSRILLYTHYQLLHRRLPLRDQVSPLRRAYQFGAKAGSAFNAACAAATLCIVRFLA
                     GAEELRSLTRDMAAFADRVRLLRQNQVLNWHEPYEQTVWNLVQDTPEPTRLHGPVYDE
                     RERSGRHLANNDAPALFNLRFCKAYLAYLFGDFQEAYRVSRENAGFLQAVAVHTLMIG
                     PALLLDALSRLAVAAELSPEERDAELQLVRATLQTFERLRSECASNSQHKYHLIAAEL
                     ARVEGRHEAARDHFTEAMSAASRSGNYAEEALTHEVSARFFLASRDPEAARAALRTAH
                     DAYLRWGATTKVKMLEGEHPNQLPNLPWGTLNTSTLATIHRSTRDFNWLDVLSALHAS
                     QTISREMSVDRLIVSLMDLVVGTAGAELGYLFVNQADGWVVQADKPGPQEPSVLVPLV
                     PLETHALTKSNGLPTSILQHVIATKEPLVLDDAATHEVFGYDSYVASRSVKSVLCFPL
                     LRQGELVCAVYLENNLVRTAFSPSRVLILEFLAGQALISLENARLYATLEQKVQQRTQ
                     QLRRKNEELAQTLDQLKEAQNCIIVQDRLASLGAMTAGLAHELRNPLNFVQNFARLSL
                     RGAEQMISRLELLGATPTNDHSAAPQAATALQAEVPALLQSVTRMKGNLEKVTLHGDR
                     IDGLIRSMLDHSRSNTGPRRDTDINALLETYANLAQHGSSTQDARLNVVLELDRTIEP
                     LPIVPQELGRVFLNLIDNARYATHKKALVAGERDYSPTLRLSTRNCGAHVEIRVRDNG
                     TGVEKSVQSRIFDPFFTTKPTGQGTGLGLSISHDIVVQANGGSLRFESEEGEYTEFII
                     ALPKRPVAQSATGRSP"
BASE COUNT          912 a         1527 c         1610 g          921 t
ORIGIN      
        1 acctccctcc gggatccttg gagagcgcac gagcaaagaa gcgatccaac tcgcgcggta
       61 ggctcgcgac cagtgatgtc ggcggttccg gctcgcgcgt gcagatccga atcagagtgg
      121 cgaccgcgtt ttctcctcga aatgggaggc gacccgtcag cgcttgataa gcgatggcgc
      181 cgagggacca gagatcgctg cgatggtcga cggccagccc ttccgcctgt tcggggctca
      241 tgtacgccgg ggtacccaac agctgatttt gtcgcgtaag gtgactgtct tttgttgcca
      301 gctggccctg gatcgacgct acgccgaaat ccagaatctt caccacggac tcggtgccat
      361 gtgacgtaat gaagatgttc tcgggtttca aatcacgatg cacgatgcgt gcagaatgtg
      421 cagcatcgag cgcggccgcg acttgtacga gaatgggggc gaaggctccc ggcgtcaggg
      481 aggtgacacg ccccaagcgg tgcccgagat cctccccctc gagcagctcc atgacggtat
      541 agagcgagcc gtcctcagcc aggccgtggt catagatctg gaccacgttg ggatgacgaa
      601 gtgcggcaat ggctcgggct tcccgcgcga aacgctcacg cgattccggc gaagcttggg
      661 cggtgccgtg catgcgtttc agcgcgacct cgcgctccaa aaccgtatca tgagccgcga
      721 acactcgacc catgcccccg cttccgagca cacgcccgag acggtacttg ccgatcgtag
      781 tctccatcag ggacagccct cttccgattg agcccccgga gtctacacga acggttcgga
      841 agtttgaagc atgggagccc aagccatcca gccatgtgga agaaggaggc tgcgcgtccg
      901 catcgagcgc tgtggtttcg agtccacttg cagccccacg ggtgcggtca cgcgaggcag
      961 ggtcatgtcc ttgcgcggca tcgccatgcc ttggtgaggc atcgccatgt ccttgcgagg
     1021 ctcggcctcg acgatctacc gccccgccca tggcaggacc ctcccaggtc gcctgccgct
     1081 cattcgcact gggattgtaa cgccgtgcgg acacgtctgt tgattcctac ggtttacggc
     1141 gaacggccgg tcgcactctg tgccaccggt cgcttcggca atgcgatgat gaactccgta
     1201 tactcaccct cttcactttc gaagcggagg ctaccaccgt tggcctgcac gacgatgtcg
     1261 tggctgatgg atagccccaa gcctgttcct tgcccggtgg gcttcgtagt gaaaaaggga
     1321 tcgaagatcc gggattgcac gctcttctcg actcccgtgc cgttgtcccg cacgcgaatt
     1381 tccacgtggg cgccgcagtt tcgggtggag agtcggagcg tcggagaata gtcacgctcg
     1441 ccagcgacga gcgccttttt gtgcgtggcg taacgtgcat tgtcgattag gttcaagaac
     1501 acgcgcccga gctcctgcgg cacaatcggg agcggctcga tggtgcgatc gagctccagc
     1561 accacattca accgcgcgtc ttgcgtggat gagccgtgtt gggcgaggtt ggcgtaggtc
     1621 tcgagcaagg cgttgatgtc cgtgtctcgg cgggggccgg tgttcgatcg cgagtgatcc
     1681 aacatcgatc ggatcaagcc gtcgattcga tcgccatgca gggtcacctt ctccaagttg
     1741 cccttcattc tcgtaacgga ttgcagcaac gcggggactt cggcctgcag tgccgtcgcg
     1801 gcttgcggag cggccgagtg atcgttcgtg ggtgtcgccc cgagcagctc caatcgggaa
     1861 atcatctgct ccgcgccgcg cagcgagagc cgcgcgaagt tctggacgaa gttgaggggg
     1921 tttcgtagct cgtgggcgag gcctgcggtc atggcgccca aggaggcgag tcggtcttga
     1981 acgatgatgc agttctgcgc ttccttgagt tggtccaagg tctgcgccag ctcttcgttt
     2041 ttgcgccgga gctgctgagt gcgctgctga accttttgtt ccagagtcgc gtagagccgg
     2101 gcgttctcga gagagatcag ggcctgcccg gccaagaact ccagaatcag gacgcggctc
     2161 ggcgagaacg cggtccgcac caggttattc tccagataga ccgcgcacac gagctcgccc
     2221 tgccgcagta gcgggaagca cagcaccgac ttcacggagc gacttgcgac atagctgtcg
     2281 tacccgaaca cctcgtgggt agccgcgtca tccaagacca gcggctcctt cgtcgcgatg
     2341 acatgctgca ggatgctcgt gggcaggccg ttcgacttcg tgagcgcatg ggtctccagc
     2401 ggaaccaaag gcaccagaac ggagggttcc tgcgggcccg gcttgtctgc ttggaccacc
     2461 cagccgtcag cctgattcac gaacagatac ccgagctccg cgcccgcggt gccgaccacc
     2521 aagtccatca acgatacaat caagcggtcg acggacatct ctcgcgagat ggtttgcgac
     2581 gcgtgcagcg cgctcagcac gtcgagccag ttgaagtcgc gtgtacttcg gtgaatcgtc
     2641 gcgagcgtgc tcgtgttgag cgtgccccag ggcaaattcg ggagttgatt ggggtgttcg
     2701 ccttccagca tcttcacttt cgtggttgca ccccaacgca gataggcatc gtgtgcggtg
     2761 cgcagcgcgg cgcgtgccgc ctcggggtca cgcgaggcga ggaagaaccg tgcgctcacc
     2821 tcgtgagtga gcgcctcctc cgcgtagttg cccgagcggg aggccgcaga catagcttcc
     2881 gtgaaatggt cgcgcgcggc ttcgtgccgg ccctcgaccc gcgcgagttc ggcggcgatc
     2941 aagtgatact tgtgttgaga gttgctcgcg cactcgctgc gcagacgttc gaatgtctga
     3001 agcgttgcgc gaactaactg gagctccgcg tcgcgctcct cgggggagag ctcggcggcc
     3061 accgcgagac ggctcagggc gtccagcagt agcgcgggcc ctatcatcag ggtatgaacc
     3121 gcgacggcct gcaggaaacc agcgttttca cggctgacgc gatacgcctc ctgaaagtcc
     3181 ccgaacaggt atgcgaggta ggctttgcag aagcgcaggt tgaagagcgc gggggcgtcg
     3241 ttgttcgcca agtgacggcc cgagcgctcc cgctcgtcgt atacggggcc gtgcagccgc
     3301 gtgggctcgg gggtgtcttg gaccaggttc cagaccgttt gctcgtaggg ctcatgccaa
     3361 ttcaacacct ggttctggcg cagcaaacgc acgcgatccg caaacgcggc catgtcccgc
     3421 gtcagcgacc gaagttcctc ggctcccgcc aggaaccgga cgatacagag cgtcgcggcc
     3481 gcacacgcgg cgttgaaggc gctgcccgcc ttggccccga actggtacgc tcttcgtaag
     3541 gggctgactt gatctctcag gggcagtctg cggtgcaata attggtaatg ggtgtagagc
     3601 agaatgcgcg acgagatttc gtcgttgccg aagcgctcgg cgatggccag cgcgagctga
     3661 gccaatcgca tcgccgactc gaagcgattc tggccgagct ccagcacccc cagaatcgtg
     3721 atggcgaacg ccgagtaagg cgttagcccg aagcgtaggg tcaggccgca gcacaggttc
     3781 aagagtgctg gcagctctgc gcttccggca tagaacgcag tcaagatcgc cggatcgagc
     3841 agctccatac ccagcaaggc tcgcgcgtcc gtgcacgcag gaaggctcag aattcgttca
     3901 tcggagagcg cacaaatggc gaccacctcc gcctgactgc cgagcggctc ggcgggccag
     3961 aaactcaggc tcgtcagggc acgcaggtac gtctccaggg cgcgcagccc atctcctcgt
     4021 gcgtaagcca cgtgccccgc caaaccgagg atcttcgcct cctccaatgg cgccaacgac
     4081 tgactcaggg cctgctgcgc aagatcggtg aacgtgtcga acttgccgca agaaaacgcg
     4141 gcctcgcaag ccgtggtctg cagctcgtgg cgcaggggct ccgcgttgcc accctggcgc
     4201 aggagatcga tcccggtggt tgcgtaagac aaagcgcgtt gggcgctgct agccgcgatg
     4261 gagcgcgtgg cggcgagcag attgagacga gctcgttcga gccgctcgtc gggactgggc
     4321 ggatcgccgc tctgattgag ctgacccacg agctcgaaca gcatggggcc ttccgccgcc
     4381 tgggggtggt tggcgagggc actcgcgatc tgacgatgca gcgcctcccg ttggggcggc
     4441 ggtaggcgac ccagaattgc ctcgcgcacg cggtcgtgcg caaagcgaag cgagctctcg
     4501 aggttgggcc cgctgagatc ggcaggcgaa tgaatgtacc ccgccggaat gaggtatccg
     4561 gcgtcgatcg cgggccacag atcggtttcg agatccaagc gcggctccac acgccgcaag
     4621 gtctcgagcg gaaattgact gccgaggcag gccgcgagct cgagcaacgt atgcgtgcgg
     4681 ttcgggagcc ttcggatttg attcgacaga accttggcaa tattctccgt gaccccgcgc
     4741 tctcgtagtt cagtcatcga ccaagcccat ccgttcgggc caaagcgcag tgcgccatcc
     4801 tcgaccagcg tcttgacgaa gatgcgcagg aagaaggggt tgcctcccgt gcgggcgtgc
     4861 accaacgagg ccaagtcacg aactgcatca cgcggcatcg agagcgcatc tgccaatagc
     4921 tcttcgacgt cgaatggggc aatcggtggg acctcgatgc gcgtgacggt
//
LOCUS       DASTKU010000629         3310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1907954, whole genome shotgun sequence.
ACCESSION   DASTKU010000629 DASTKU010000000
VERSION     DASTKU010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907954"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VFQ61_21990"
     CDS             <1..1309
                     /locus_tag="VFQ61_21990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9957191.1"
                     /translation="ALQLLKDHHIDLVITDLRMPKLDGMELLRRIVAMDEEMPVVMLT
                     AHGTVDNAVEALKTGAFDYITKPFDQAEVRTIVRKALRTKDLSLEDASRAAQGPTSGA
                     RYGIIGHSAGILDLYAVLDRVADTPTTVLITGESGTGKELVARALHENSSRREKPFIK
                     VNCAAIPKDLMESELFGYERGAFTGAVTSKPGRFELATGGTLFLDEIGSIPVEMQVKL
                     LRALQESEFERVGGIKTIRVDVRVVAATNSDLKKEIAQGSFREDLYYRLNVVPIRLPA
                     LRERSQDVPELVRHFIEKFNARLKKSVTGIDGQALGLLCGYPWPGNIRELENVIERAV
                     LFCDRTEIGTPDIPAEVRGFSAVQSTPQAPPVALTGASSDGLKEQVKAAMSRLERELI
                     VRALEQTHGNVTHAARLLKISRKGLQLKMKELSLRERDDNKEP"
     gene            1306..2568
                     /locus_tag="VFQ61_21995"
     CDS             1306..2568
                     /locus_tag="VFQ61_21995"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9957192.1"
                     /translation="MKSARSAALPLSLVVVLGVAQSVAGCRGADSGAGSSSLGPGASS
                     SARGLGSAAARAVGSIGPPERPPPPRQGMVWIPGGALVAGTPPDQLPRKVDEEMRGEQ
                     VILHGFYMDVFPYPNEEGAIPLTNVTRDEALALCAERDKRLCTELEWERACKGPSNRI
                     YEYGDRHRPDRCGTGVAPSLRPAGLRVGCRSDFGIRDLHGGAFEWTSSSFGRGTSGLA
                     TVRGGNGTAGEVAARCANAESRTPESKSGLIGFRCCAGEPNPTEVTLNVRWGKRLDEF
                     ERIDKPTSTRLLAALPEEATRALARSGSPRIERLWVWRPVGNEDLSAAAVCSGIGTRP
                     VCGVLIAREELGRATSVAWAPSGLYIASLHMERDPKDVWLLGGDEVGRFKRLIAYAWG
                     KVTVHGEDRRVGKEDTEKKSKSRSKGKK"
     gene            2646..>3310
                     /locus_tag="VFQ61_22000"
     CDS             2646..>3310
                     /locus_tag="VFQ61_22000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957193.1"
                     /translation="MKSRLVEVWERLVRAGRRLATRRSFPGWVAFGAGVVVLVALAVH
                     QMALVGDFRIDDAYITFSFSKNLAAGHGPIFSHGVRVEGYSNFLWMLICAAGYLLAPN
                     ADPYWWARAAALVFLVGALRAVYRLVRPEAGTVAGFLAMGAVLVNTDVMRAALSGLET
                     VPFMACIVLGWSAYLRATPPLHPKSLAWFLAAALMRIDGFVPLLIVLGFEFVSALNDR
                     RFK"
BASE COUNT          558 a          972 c         1171 g          609 t
ORIGIN      
        1 agctttacag ctgctgaagg accaccacat cgatttggtg atcacggatt tacgaatgcc
       61 gaagctcgac ggcatggagc tgctgcgccg catcgtggcg atggatgaag agatgccagt
      121 cgtaatgctg accgcgcacg ggaccgtgga caacgcggtc gaggcgttga agaccggcgc
      181 cttcgattac atcaccaagc ccttcgatca ggctgaagtg cgcacgatcg tgcgtaaagc
      241 gctgcggacg aaggacctat cgctggagga cgcgtcgcgt gcggcgcagg gccccaccag
      301 cggcgcgcgc tacggcatca tcggtcacag cgcgggaatt ctggatttgt acgcggtgct
      361 cgatcgcgtg gcggacacgc cgaccaccgt gctcatcacg ggcgagagcg gcaccggcaa
      421 agagctggtg gcccgcgcgc tgcacgagaa ctcgtcgcgc cgtgagaagc cgttcatcaa
      481 ggtcaattgc gcggcgatcc ccaaggactt gatggagagc gagctgttcg gctacgagcg
      541 cggggccttc accggtgcgg tgacgtccaa gccgggtcgg ttcgagcttg ccactggagg
      601 caccctgttt ttggacgaga tcggctcgat cccggtcgag atgcaggtga agttgctgcg
      661 ggcgctgcag gagagcgagt tcgagcgcgt gggtgggatc aagacgatcc gggtggacgt
      721 gcgggtggtc gccgccacga acagcgatct caagaaggaa atcgcgcagg gcagtttccg
      781 ggaagacctc tattaccggc tgaatgtggt gccgattcgc ttgcccgcct tacgcgagcg
      841 atcgcaagac gtgcccgagt tggtgcgcca tttcatcgag aagttcaacg cacgtttgaa
      901 gaagagcgtg accggcatcg acggtcaggc gctggggttg ctttgcggtt acccatggcc
      961 cggcaacatc cgcgagctcg agaacgtgat tgagcgggcg gtattgttct gtgaccgcac
     1021 cgagatcggg acgccggaca ttcccgccga ggtgcgcggc ttttcggcgg tacaatcgac
     1081 gccccaggcg ccgcccgttg ccctgaccgg cgccagcagc gatggtctga aggagcaggt
     1141 gaaggcggcg atgagtcggc tcgagcggga gctgatcgtt cgagcgctgg agcagacgca
     1201 cggcaacgtc acgcacgccg cacgtttgct gaagatctcc cgcaagggcc tgcagctgaa
     1261 gatgaaagaa ttgtctttgc gagagcgtga cgacaacaaa gagccgtgaa gtcagcgcgt
     1321 agcgcggccc tgccgctctc cctcgtcgtg gtgctcggcg ttgctcaatc ggtcgcgggc
     1381 tgtcgtggcg cggactcggg agcgggctcg agctctttgg gccctggcgc ctcgtcgagt
     1441 gcacgcggcc ttggctcggc tgcggctcgc gccgtgggat cgattggccc gcccgagcgc
     1501 ccgcctccac cgcgccaagg catggtgtgg atccccggcg gagcattggt ggcaggcacg
     1561 ccgccggacc agctgccccg caaagtggac gaggagatgc gcggggaaca ggtgatcctg
     1621 cacggcttct acatggatgt gttcccctat cccaacgagg agggggccat tccactgacg
     1681 aacgtcacgc gcgacgaagc gttggccctg tgcgccgagc gcgacaagcg gctctgcacc
     1741 gagctcgaat gggaacgagc ctgcaaaggc ccctccaatc gcatttacga gtacggagat
     1801 cgtcaccggc cagatcgctg cgggacggga gtggccccga gcctgaggcc agccgggttg
     1861 cgggtgggtt gccgaagcga tttcggcatt cgcgacctgc atggcggtgc attcgagtgg
     1921 acctccagct cattcggccg aggtacgagc gggctcgcga ccgttcgcgg cggcaacgga
     1981 acggcgggtg aagtcgccgc gcgttgtgcc aacgccgaga gccgcactcc cgagagcaag
     2041 tctggcttga ttgggtttcg ctgttgcgcc ggggagccca acccaaccga agtcacgttg
     2101 aacgtgcgct ggggcaagcg gctcgatgag ttcgagcgga tcgacaagcc cacgagcacg
     2161 cgtctgctgg cagcgctgcc cgaagaagcg acgcgcgcct tggcgcgcag tggaagcccg
     2221 cgcatcgagc ggctctgggt gtggcgcccg gtcggcaacg aagatctctc ggccgccgcg
     2281 gtgtgttcgg gcatcggcac gcgacccgtc tgtggcgtgc tgatcgcgcg agaggaactc
     2341 ggtcgagcga cgagcgtcgc atgggcgccg agtgggctct acatcgcgtc tttgcacatg
     2401 gagcgcgatc ccaaggacgt gtggttgctc ggaggagacg aagtgggccg attcaagcgc
     2461 ctgatcgcgt atgcgtgggg caaagtgacg gtccacggtg aggatcgccg ggttggcaaa
     2521 gaagataccg agaagaagtc caagagccga tccaagggca agaagtagcg cacgacctgc
     2581 acggtgattc gacgcgggtg cgagttttca gcgccgcggt ctttctgcta gcgtccccct
     2641 cgtcgatgaa aagtcgtctg gtcgaggttt gggagcgtct ggttcgtgcg ggtcgacgcc
     2701 tggcaacgcg tcgcagtttt cctggctggg ttgcgtttgg cgcgggtgtg gtggtgctcg
     2761 tcgcgctggc cgtgcaccag atggcgttgg tcggtgattt ccggatcgac gacgcctaca
     2821 tcacgttctc cttttcgaag aacctggccg ccgggcacgg ccccatcttc agccatgggg
     2881 tgcgcgtcga ggggtactcg aacttccttt ggatgctgat ttgcgcggca gggtacttgc
     2941 tggcgccgaa cgccgatccg tactggtggg ctcgcgccgc agcgctcgta tttctcgtcg
     3001 gtgcgctgcg cgccgtttac cgcttggtta gaccggaggc gggcaccgtc gcggggtttt
     3061 tggcgatggg cgcggtgttg gtcaacacgg acgtgatgcg cgcggctctg tcgggcctcg
     3121 aaaccgtgcc cttcatggct tgcatcgtgc tcgggtggtc cgcgtatctc cgggcgaccc
     3181 cgccgttgca tccgaagtcg ctggcttggt tcctggcggc cgcgctgatg cggattgacg
     3241 gcttcgtgcc gctcttgatc gtgctcggct tcgagttcgt gagtgcgctc aacgatcgcc
     3301 gcttcaagat
//
LOCUS       DASTKU010000630         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1683706, whole genome shotgun sequence.
ACCESSION   DASTKU010000630 DASTKU010000000
VERSION     DASTKU010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683706"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="VFQ61_22005"
     CDS             complement(<1..1309)
                     /locus_tag="VFQ61_22005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957194.1"
                     /translation="MSTVPIRLLFTLAIGAAAGTLAPSARALCDQSLAARDESDQAFF
                     DARQAAIDPATKCVTNSAAYQNFDLWLPRRLQMADQAVARGVDPGGWSFRGKSEPCEM
                     RFEWGKTVNAAALVLLGAPIGATLDPQDRPFHSAGDYFALIVGEESAWHDSYQHLIED
                     SLGGALGQTSQARPFEPLTVTTSCAVYQAPGPLYADGNDVAGRASVLVHEGWHTWQDA
                     HLDTWIEPLFEVNVPIHHDNDWDARANTSFCGDIGFTEHPECDWYHPHLNSEFGPGEL
                     ERAGSDGFTEHSVFQVQQEFLCDIADYSPDVVPASVRELAALHAKANFVQFFANQPPM
                     FCGSTRPYWTKDRRALADKIGTSVPECKYTYGTPVGASLPAVAGANAPKDCSGTDIAA
                     VDTLLPCTSGLTDCSATRICGAGMTCQSDADGACCRPACSNPNQ"
     gene            complement(1342..>3416)
                     /locus_tag="VFQ61_22010"
     CDS             complement(1342..>3416)
                     /locus_tag="VFQ61_22010"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HET9957195.1"
                     /translation="PDATSPAGARIVSKGGPGQYPFQLAYKSAYYANPEKLSWALGLT
                     PTGARYDYSDQWLTPGQWAHVAMVYDRARVALYIDGREATPAIEHTDPLVHAPGTGLF
                     IGALYNAKYPFKGAIDEVRIYDRALTSKEVAANACSPTVSNGGGGGGAGGAGGAGGTT
                     GGQPSGGAAGEASSCPVVPGYVGRWSFDEGAGETARDDSVFANTGVLGSGMAARRPAW
                     APGKFGSALSFDGIDDVVGCGYGASLDLAGPVTIEAWIKPTAASVAGGRIVSKGRISN
                     YPYELTYKSLYGQHKETVGWAFNFNQTGAKYGNTNEWLVPNEWAHVALVYDLTSIRLF
                     INGAPATPAVPHTEELQRSPYGSLLIGSHDNGTFPFQGLIDEVRVYDRALSPAEVATN
                     AQCAGRPGTVGQACQDSSQCDAGAVCGRANGGYFGLAAAQDACWAPACEDAGFRMQHC
                     GTSGALCGACRCVGCPSDEDLLAAARGSADNPGPAAPPPPLGGTGTTAVSSVRDFIEW
                     TLQSRSSQVLDGRAVIATAAGNSDVANALIAEVNVEQGSLVRNLIVVGILGELKSPEG
                     EAFMRFLVDRPVPPGAATSREVLRLTAVQAKAVDGLAFSRSLGAKRKLLQVAAAHASR
                     LVRFEAMRAYLDRFGPEAKNDLLGVVRPEEQLFLDGFYHRSELKNDLSFDQKLTQYLV
                     THPGGGVP"
BASE COUNT          590 a         1140 c         1086 g          600 t
ORIGIN      
        1 cctggttcgg gttggagcac gccggtcgac agcacgctcc atccgcgtcc gattggcacg
       61 tcatgcccgc accgcatatg cgcgtggcgc tgcaatcggt cagcccggag gtgcaaggaa
      121 gcaacgtatc gacggccgcg atatccgtcc cgctgcaatc tttgggcgcg ttcgcgccgg
      181 ctacagcggg taacgacgct ccgacgggtg tgccataggt gtacttgcac tccggaaccg
      241 aagtgccgat cttgtcggcg agcgcgcgtc gatctttggt ccaatatggc cgcgtgctac
      301 cgcagaacat cggcggttga ttcgcgaaga actgaacgaa gttcgccttc gcgtgaagcg
      361 cagcgagctc gcgtacgctc gcgggaacca cgtcggggct gtaatcggcg atgtcgcaca
      421 gaaactcctg ctgcacctgg aacacgctgt gctccgtgaa accgtccgag ccggcgcgct
      481 ccaactcccc cggaccgaac tcgctgttca agtgcgggtg gtaccagtcg cactccggat
      541 gttctgtaaa gcctatgtct ccgcaaaagc tcgtgtttgc tctcgcgtcc caatcgttgt
      601 cgtggtggat gggaacattg acttcgaaca agggctcgat ccacgtgtcc aagtgcgcgt
      661 cctgccacgt gtgccagccc tcgtgaacga ggaccgaagc gcgacccgcc acgtcgttcc
      721 cgtcggcgta caggggtccg ggcgcttggt acacggcgca gctcgtcgtc accgtcaacg
      781 gctcgaaggg gcgcgcctgg gacgtttgcc ccaatgcgcc gcccaaagag tcctcgatga
      841 ggtgctggta ggagtcgtgc caggcggact cctcacccac gatgagcgcg aagtagtcac
      901 ctgcgctgtg aaacggccga tcttgggggt ccagcgtcgc tccgatcggc gcgccgagta
      961 acacgagcgc ggccgcgttg acggtcttgc cccactcgaa gcgcatctcg cacggctcgc
     1021 tctttccacg aaatgaccaa ccccccggat cgacgccgcg ggcaacagcc tggtctgcca
     1081 tctgcagtcg cctcgggagc cagagatcga aattctgata ggcggccgaa ttcgtaacgc
     1141 acttcgtcgc gggatcgatg gcggcctgcc gagcgtcgaa gaacgcttgg tccgactcat
     1201 cgcgcgccgc gaggctctgg tcgcagagcg cccgtgcgct gggtgcgagc gtacctgcgg
     1261 cggctccgat tgcgagcgtg aacagaaggc gaatcgggac tgtgctcatc gacttgccga
     1321 catggtttac gaaacgcgcg ttcacggaac gcctcccccc gggtgcgtga ccaggtattg
     1381 cgtgagtttt tggtcgaaag agagatcgtt cttgagctcg ctgcgatgat agaagccgtc
     1441 gaggaagagc tgctcttcgg gacgaacgac accgagcaga tcgttcttcg cttcgggccc
     1501 aaacctatcg aggtaagcgc gcattgcctc gaagcggacc aagcgcgaag cgtgagccgc
     1561 ggctacttgg agcagcttgc gttttgcacc caggctgcgc gagaaagcga gcccgtccac
     1621 cgctttggcc tgtaccgcgg tgagccgcag cacttcgcgg gaggtcgccg cgcccggcgg
     1681 caccggtcgg tcgacgagga agcgcatgaa cgcctcgccc tcggggctct tcaactcacc
     1741 caggataccg accacgatga gattgcggac gaggctgccc tgctcgacgt tcacttccgc
     1801 tatcaacgcg ttggcaacgt ccgaattacc cgccgccgtc gcgatgacgg cgcggccgtc
     1861 cagcacttga ctcgaccggg actgcagcgt ccattcgatg aagtcccgca cgctgctcac
     1921 cgcggtcgtc ccggtgcctc cgagcggcgg gggcggcgct gcggggcctg ggttatccgc
     1981 ggagccacgc gcggccgcga gtaagtcttc gtccgagggg cagccgacgc agcggcaagc
     2041 gccgcacagc gccccggacg tgccgcaatg ctgcatccgg aaaccggcgt cttcgcacgc
     2101 gggcgcccag caggcgtcct gggcggcagc gagcccgaag tagccgccgt ttgcgcgacc
     2161 gcacaccgca ccggcatcgc actgcgaaga gtcttggcag gcttgaccca ccgtgcccgg
     2221 cctgccagcg cactgagcat tggtcgcaac ctccgccggg ctgagagcgc gatcatacac
     2281 ccggacttcg tcaatgagcc cctggaatgg gaacgtgccg ttgtcgtgcg aaccgatcag
     2341 cagactgccg taggggcttc tctgcagctc ttctgtgtgc ggcacagcgg gcgtcgccgg
     2401 cgcgccgttg atgaagagcc gtatgctggt caggtcgtaa acgagcgcga cgtgcgccca
     2461 ctcattgggt accagccact cgttcgtgtt gccatacttc gcccctgtct gattgaagtt
     2521 gaaggcccag ccaaccgttt ccttgtgttg tccgtacagg ctcttgtaag tgagctcgta
     2581 cggataattc gatatgcgac ccttcgagac gatccggccc cccgcgacgc tcgccgcggt
     2641 gggcttaatc caagcctcga tcgtaacggg ccccgcgaga tcgaggctcg cgccatagcc
     2701 gcagccaact acgtcgtcga tgccatcgaa gctcagcgcg gaaccgaatt tgcccggagc
     2761 ccacgcaggc cttcgtgcgg ccattccgct gccgagcacg cccgtgtttg caaagaccga
     2821 atcatcacgc gccgtctcgc ccgcaccctc gtcgaaggac caacgaccga cgtagccggg
     2881 cacgacggga cacgacgacg cttcgccagc ggcaccgccg ctcggctgtc cgcccgttgt
     2941 gccgccggcg ccgccggcgc ccccggcgcc cccacctccg ccgccattcg agacagtcgg
     3001 cgagcaagcg ttcgccgcga cttccttgct cgtgagcgct cgatcgtaga tgcggacctc
     3061 gtcgatcgcg cccttgaagg gatactttgc gttgtagagt gccccgatga aaagcccagt
     3121 gccgggcgcg tgaacgagcg ggtcggtgtg ctcgatcgcc ggtgtcgcct cacgcccgtc
     3181 tatgtagagc gctacgcgcg cccgatcgta gaccatcgcc acgtgcgccc attgccccgg
     3241 cgtaagccat tggtcggagt agtcatagcg agctcccgtc ggggtcagcc cgagcgccca
     3301 ggagagcttc tccggattcg cgtagtaggc agacttgtaa gcgagctgga acggatactg
     3361 acccgggccc cctttcgaaa cgatgcgggc cccagccgga ctcgtcgcgt cggggt
//
LOCUS       DASTKU010000631         8953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1461349, whole genome shotgun sequence.
ACCESSION   DASTKU010000631 DASTKU010000000
VERSION     DASTKU010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461349"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            984..1466
                     /locus_tag="VFQ61_22015"
     CDS             984..1466
                     /locus_tag="VFQ61_22015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957196.1"
                     /translation="MDSVPDLASRSLAAPRDMAPRRLIRYHRAMGITAKIWADSEIEV
                     SAMLADPKRVGSRFYAKPTDEYALIHSARSRGLDQYLASKAAHGGGLGAIPGASNITY
                     VLPADRVRVLAETPTSSPFHRLLTDYPTDAINRVEIADVVRAAAGCGHGLVFCLFEDW
                     "
     gene            1538..1969
                     /locus_tag="VFQ61_22020"
     CDS             1538..1969
                     /locus_tag="VFQ61_22020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957197.1"
                     /translation="MTVPHSPYQMHAIPRIDGSDSVVAWFGCWPRFHDHYLVSAPTCV
                     APVGTLCIHAWRTQWELTDAAGRFLQDHHCLVTFDLRRVECVELTELVFPAIIFELDV
                     CARSDAEGWAIQWSSSYGAEGRILVEGVSVKVEPRNPERKE"
     gene            complement(2024..2413)
                     /locus_tag="VFQ61_22025"
     CDS             complement(2024..2413)
                     /locus_tag="VFQ61_22025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957198.1"
                     /translation="MALAGDSPDEMFAQWQKLIAPHSREALASYPAAKERYLRGLPPG
                     ENFFVTTILRDEQHRFEQVFVLVDRIERGKVTGRIASDILTVAGHRRGGTLTFEETEV
                     VDWLIAKPDGSEEGNFVGKYLETLSSK"
     gene            2920..3684
                     /locus_tag="VFQ61_22030"
     CDS             2920..3684
                     /locus_tag="VFQ61_22030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957199.1"
                     /translation="MPTEQELRNNPVYQRQQVRNALASLENEPEELLANVTLTCSAGA
                     GHQTVLNPAGGFALNAAGDQLKIGINAPAAHYPHRLVIPMLETQTRSSAGAGLAQRLA
                     ASPRFAIGGAARVWITDQQTGCTVLALDWGGGQYSMFHLLPYENAAFGALARASFFVS
                     KAARSVIKNSSLRSEATQVVTASQNGGAPPQRYILLQSQHNINKGRLMQVVGVKRNGG
                     WDFFRQVQTMTPAGPRVHSVKHAPWRPWTERFYHDV"
     gene            complement(3757..4980)
                     /locus_tag="VFQ61_22035"
     CDS             complement(3757..4980)
                     /locus_tag="VFQ61_22035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957200.1"
                     /translation="MRDPQCPDGAAQRRIGRRAFTPRSWLRAVCLVALACCTGSARAA
                     AGYVRVYIPQCEPQVLNPTAVLQAIRVEVPPAKLELVSGLDSSGQPAPAQLVSRLLVI
                     APERCQTPVSAVTLALFSRAETEPALRHLQLSDVDQGNEPRVIAIAVAELLNQHSIFP
                     LPAASALGVLPEEPRLPGAEERTQSQPLHPRSPERDTTSASAVRLGGEHFVVRRSAAG
                     NLAGAGLPSDSVIGSTPARLSSRFAGMTLLARAFPSGKLALVGPALTGCARANGLLSL
                     CARSEFTFGSAGLYSDDANGAVDNIRTRSAVASIGPSLMSGGPIELGIGLRVSAGIAE
                     LDSSQRRKRGFLSLIGAESTLRGRIAPHVSALIGIEIGSTLSGIHLENTSPDHGAAVS
                     FGGAFLAVQLGLAVD"
     gene            complement(4958..7594)
                     /locus_tag="VFQ61_22040"
     CDS             complement(4958..7594)
                     /locus_tag="VFQ61_22040"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9957201.1"
                     /translation="MTFGATRIRRFVGGWRAEEVGAVASVLVLLCCRILLSATIRWNT
                     YFTTRGAAIFAFIATFYVLGGSRSVGAKCRPYQRALQFLRDWGPFVVLIAVYENLLPL
                     IRVFRPHLYDAQMLRWDLYMLGTTPSVWLVPLASRTRTEWFSACYLALFALPLISGGV
                     LYLSGRMREFRQFLRTFVLAGAMGYLGYVAVPVVGPRYFFPELFPGRGVEGGSFSALT
                     DHVSHPAQIASSVVPNCFPSLHTAWGIIVLIFAYRQCRWLFWFYLIPVTSLIFATLYL
                     RFHYAVDVIAGATLGIGLSVAVPWLEARYANGATAGLRITPARTGPQGTPRGARSEGV
                     RLLLSAAARLKPTRAFVALLAVVPWIYASSLANGLTLKNRAQDGAELVTAAITSGTAH
                     PPGYPLYLMLVRAACWFWAGAAPINVAHASSAVFCTIAGLLVCAIVRKVLAAIAPDVN
                     DAARDLAGVCAGGLFCLSRTVLGVALVAEVYALHAALVAGMSLCAVNLLVTSEREVSR
                     GTAVSFGLLGGLAISHHLTALPVFAVQCLILWRSRGTSRNDLRVFLPWVAGCCLGLLP
                     WLYLPWAASREPVVSWGQPSTLASFWWVVSAAQYHARLGGTPSELWKRLIEYGPFQAL
                     PASALALAWLGLACLYRTWLNSRGRVRGSRQDALTACRPRLAVIALLAGSGAVNLVLC
                     LFYDIPDLGNYFIPALLGLTILAGVGCSELMRALRELRGTRGAAFEIVSCLVGATVWW
                     TAVSNLRQTDAQGGTRFDQRVRTIVRQAAPSSLIAARGDGMVFGLWYEKFVRQPRHDV
                     DVVSRELLLQAWYPRQSRHFSERMRWPAERLQGDPDRRLAAVIRENWAHRPVVVIDEK
                     DVPSGCVRAQNWVVRCEGSAMP"
     gene            7984..8496
                     /locus_tag="VFQ61_22045"
     CDS             7984..8496
                     /locus_tag="VFQ61_22045"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9957202.1"
                     /translation="MQATRTFIDAPLGSPRSEAAAEEDTDLVKEALAGNRWAEEALYY
                     RHVRGVTRVVLRLLSRSSEVEDVVQDTFVIAFGDLAVLADPAAFRHWLIRIAVRQVHR
                     RFRRRKLLRLLGLDASQDDQILAAQADPKAGPETRVLLGEVDRILAKLPIRARVAWVL
                     RMWRDRNSRT"
     gene            8597..>8953
                     /locus_tag="VFQ61_22050"
     CDS             8597..>8953
                     /locus_tag="VFQ61_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957203.1"
                     /translation="MKQLRPVVPKRRRLPLPVSRVLADPLDERAINKMWRTIERRRGQ
                     PVHPIGKWILVLACIGSLGVLASVLMPRNQPQPLAAANNVGAKANSSAQASLLSPIEV
                     PPMAPPVDVTMADGSHI"
BASE COUNT         1863 a         2900 c         2676 g         1514 t
ORIGIN      
        1 acccgtttct gccacgtgtc ctcatgatca cccagcagcc agggcgtgag gctcgccatc
       61 aggccaacga agtagcgttt tcgcggcggc gcagcgggcg agcggagcga gccgcgaaga
      121 ccggcgttaa aacgctacct cgttgggcag cagacccctg gagaactaaa gggatcgacc
      181 ccacggaact cctcgctgga cttcgtcgaa cgagtactcc ggcgggggcg atggccaacg
      241 accgtgtgcc tcaacgatag cggacggcgt tccagatgag atcgaataga agtcctcatc
      301 gaaggcgcgg atcccgaagc gcgcaaggta gctagtcagg aggtcgcgat cgaaacgatc
      361 tcgcttcctc cgcgcggagt atcgctccag ttgctcgaaa gggaatggcg accccgaagc
      421 atcgaacgac cagcgcccgc catcgttggc gcacgacacc gagcggaggt accccaaggg
      481 cggggtgccg ccgagagatt ctggcgcgta gacctcccaa atcgtcgctg gccatcgtgc
      541 acgcgcgcta gtggagcaga cccgcatggc gcggcaccga aggtgttgag ccaggtagga
      601 cattacggga aaagggtccg agcccagtag tccgctggcg aagtatgccg tccaatcgga
      661 ccttgtagaa acgaatgcgg ccctgcgcat ctccatcgac aacggtgcga gcgcgttgaa
      721 ggtgctctcg agcgatactc tttcggatgc gaccggagtc cgtccgatgt cggcctgcca
      781 tttcacgaag gcggcaacga cgcatgcggc cggtgcttcg atgagcccga agtctgatgt
      841 ggccggatgc caaatgtcta cgaaggttcc catcgcgctg cagctctccg ccaacgtacc
      901 aggcctaagc tgcgcggatc gagcaaagcg agatcagcat tagcatcagg ccggagttgg
      961 gcggcagcac gcgcgcggaa cgtatggatt ccgtgccgga cctcgcatcg cgatccctcg
     1021 cggcgccccg cgacatggca ccgcgccggc tgattcgcta tcatcgcgcg atgggcatta
     1081 ccgccaagat ctgggccgac agcgagatcg aggtcagcgc gatgctcgcc gatccgaagc
     1141 gggtcggctc ccggttctac gcgaagccga ccgacgagta cgcgctgatc cacagcgcgc
     1201 ggagccgcgg cctcgaccag tacctcgcgt ccaaggccgc gcacggcggt ggcctcggcg
     1261 ccatccccgg cgcgagcaac atcacctacg tcctccccgc cgaccgtgtc cgcgtgctcg
     1321 cggagacgcc gacgagcagc ccgttccacc gcctcctcac cgactacccg acggatgcca
     1381 tcaaccgcgt cgagatcgcc gacgtggtcc gcgccgctgc aggctgcggc cacggcctcg
     1441 tcttctgcct cttcgaggac tggtgaagat gggcgcgcgt gatcgtcgtc gcgcgacacc
     1501 ggcggcgtcg cgccgagcag ccgtgcggag ccccgccgtg accgttccac actcgccata
     1561 tcagatgcat gctatcccac gaatagacgg ctccgattca gtagtggcct ggttcggctg
     1621 ttggccgaga ttccacgatc actacttggt gagcgcccca acttgcgtgg ctcccgtggg
     1681 cacgctctgc attcatgcgt ggcgcacgca atgggagctg actgacgccg ccgggcggtt
     1741 cctccaggac caccactgcc tagtcacgtt cgacctccgc cgggttgaat gcgtggaact
     1801 caccgagttg gtctttcccg caatcatctt cgaactcgat gtctgcgcgc ggtccgacgc
     1861 tgagggttgg gctatccagt ggtcctcttc ctacggcgcc gaagggcgaa tcttggttga
     1921 aggcgtgagt gtcaaagtcg agccccgcaa cccagagcgc aaggagtgaa taagtgccat
     1981 caccgggcgt catcaattgg cgtcattgtg cgaatcgccg cgttcacttg gagctcagag
     2041 tttcgaggta cttgccgacg aagtttccct cctctgaccc atccggcttc gcaatgagcc
     2101 aatccaccac ttcggtctcc tcgaaggtca gagtcccacc ccgtctgtga cccgcgacgg
     2161 tcaagatgtc ggaggcaatc cgccccgtca ccttaccgcg ctcgattcgg tccaccaaca
     2221 cgaagacctg ctcgaaccga tgctgctcat cccgcaggat cgttgtaaca aagaagtttt
     2281 ctcccggcgg caaaccgcgc agatatcgct ccttcgcggc ggggtaggac gccagcgctt
     2341 cgcgcgaatg gggggcgatg agcttttgcc attgagcgaa catctcatcc ggggagtccc
     2401 cagcgagtgc cacgggccga tccgccgggg cgttaggagc tagcgcgccc tcttgtcggt
     2461 gccccgtgtt cgcgcagccc acgagaacga aaccaaggca cgtgatgaga gcgcaagctc
     2521 tccagccggc tagcatcgat gacaaatgaa atctcctccc gggtgtgcgt cgcagcttaa
     2581 cccgaaccgc gccgcgatca gaggctttga ggcgaatttt ctatgctgcg ttcgggccgc
     2641 gacgtaacga gcgcgccccc cgtccaggat cttcattggc gcagccttca aaacgccaat
     2701 cacccccgca agaccgcgca ggcccccaca acatctcgtc ggcgatctcc caacaagtgc
     2761 cctgcctcga ggagatctcg ccggcttgta tgagctcaac cagctctcta cgtaaattgt
     2821 gtgaatcgat gtacgttcaa aacaattcct cgagttgact tgacccaggc tggcccggca
     2881 caatgaaggt ttgctgaaga gtgctcgaga ggaagatcga tgccgaccga acaagagctc
     2941 cggaacaacc ccgtatacca gcggcagcag gtacgcaacg cgctggcttc cttggaaaat
     3001 gagcccgaag aattgctggc gaacgtgacg ctcacgtgtt cagccggcgc agggcaccag
     3061 actgtcttga acccagccgg tgggtttgca ctcaatgcgg ccggcgatca gctcaagatt
     3121 ggaatcaatg ctccggccgc gcactacccg caccggctgg tcattcctat gctggagacc
     3181 caaacgcgtt caagcgcggg tgctggtttg gcgcagcggc tggcggcttc gccacgcttt
     3241 gccattgggg gagcggcccg agtctggatt accgatcagc agaccggctg cacggtgctc
     3301 gcgctcgact ggggtggcgg tcagtattcg atgtttcacc tgctgcctta cgagaacgca
     3361 gcatttggag cgctagcccg agccagcttc ttcgtgagca aagccgcccg ctcggtgatc
     3421 aagaactcat ccctgcgcag tgaggcgaca caagtcgtca ccgcctcgca gaacggcggc
     3481 gcaccgcctc agcggtacat cctgcttcaa tcgcagcaca acatcaacaa gggtcgattg
     3541 atgcaagtgg tgggcgtaaa gcgcaacggc gggtgggact tcttccggca ggttcaaacg
     3601 atgacccctg cgggcccccg cgtacattcg gtcaagcacg cgccctggcg gccgtggacg
     3661 gagcgcttct atcacgacgt ctgacgggaa cggagcggac ggttacgctt agttgaatag
     3721 taagggcacg ccaaaggtgc cttctggact tataccttag tctaccgcaa ggcccagctg
     3781 cactgccagg aacgcgcccc cgaaggacac cgcggcccca tggtccgggc tcgtgttctc
     3841 gagatggatt ccagagagcg tactcccaat ctcaataccg atgagtgcgc tgacgtgcgg
     3901 cgcgattcgg ccccgcagcg tgctctcggc gccaattaga gacagaaatc ctcgtttgcg
     3961 tcgctgcgag ctgtcgagct cggcgatgcc tgccgaaact cgcaatccaa tcccgagctc
     4021 gatggggccg ccgctcatga gcgagggccc gatcgacgcg accgccgacc gcgtgcgaat
     4081 gttgtctacc gccccattcg cgtcgtcaga gtacagtccg gcgctgccaa atgtgaattc
     4141 gctacgagca cacaatgaca atagcccatt ggcccgtgcg cagcctgtca atgcgggccc
     4201 gacgagtgcg agtttgcccg aagggaatgc ccgggcgagt aaggtcatgc ccgcaaacct
     4261 ggagctgagg cgagcgggcg tacttccgat cacgctgtct gagggtaaac cggcgcccgc
     4321 cagattccca gcagccgaac gccgaacaac gaagtgctct cctcccaagc gcaccgcgga
     4381 ggcagacgtc gtatcgcgct cgggggagcg tgggtgtagc ggctgcgatt gggtccgctc
     4441 ctccgctccc ggcagccgcg gctcctcggg caatactccg agagctgaag cggcggggag
     4501 cggaaatatc gaatgttgat tcaagagctc tgcgacggcg atcgcgatta cccttggctc
     4561 attcccctgg tcgacgtccg acagttgtaa gtgccgcaac gccggttcgg tctcggcgcg
     4621 cgaaaagagc gccaaggtga cggctgacac cggcgtttgg caacgttccg gcgcaatcac
     4681 gagcaggcgc gagacgagct gcgctggcgc gggttgtccg ctcgaatcga gaccagaaac
     4741 gagctccagt ttggcgggag gaacttcgac gcgaatggct tgtaggacag cggttggatt
     4801 caaaacttgc ggttcacact gcgggatata gaccctgacg taccccgccg cggcgcgcgc
     4861 gcttcccgtg caacatgcca aagctaccag gcatacggcc cgcaaccagc tgcgcggagt
     4921 gaacgcgcgc cgcccgatcc tccgttgtgc ggcaccatca gggcattgcg gatccctcac
     4981 agcgcaccac ccaattctgc gcgcgcacgc aacccgacgg aacatccttt tcgtcaatga
     5041 ccaccaccgg gcggtgcgcc cagttctcgc gaatgacagc cgcaagtcgg cggtcagggt
     5101 cgccctgcag ccgctcagcg ggccagcgca tgcgttcact gaagtggcga gactgccggg
     5161 gataccacgc ttgcaacaga agctctcgac taaccacatc gacatcgtgt ctcggttggc
     5221 gcacgaattt ctcgtaccaa agcccgaaga ccatgccgtc gccgcgcgcc gcaatcaagc
     5281 tcgaaggcgc ggcttgccgc acgatcgttc gaacgcgctg gtcgaagcgg gttcctccct
     5341 gcgcgtccgt ttgacgcaag ttggatacag cggtccacca tacggtcgcg ccgaccaggc
     5401 acgagacaat ctcgaacgcg gctccccggg tcccgcgcaa ctcgcgcagc gcgcgcatca
     5461 attcggagca acccacgcca gctaggatcg tcaaaccgag cagtgcggga atgaagtagt
     5521 ttccgagatc gggaatgtca tagaagaggc agagtacgag gttgacggcc ccgctcccag
     5581 ccagcagcgc aatgacggcc agccgcggcc tgcaagccgt aagtgcgtct tggcgcgagc
     5641 ctcgcacccg cccgcgggaa ttgagccatg ttcgatagag gcacgccaat ccaagccatg
     5701 ccaaagccag cgcggacgcc gggagcgctt ggaaggggcc atattcaatg agtctcttcc
     5761 acagctcgga aggcgtgcct ccaagccggg catgatattg cgcagcgctc accacccacc
     5821 agaaactcgc gagcgtactc ggctggcccc aagacaccac gggctctctc gacgctgccc
     5881 agggcaagta aagccagggc aacaagccaa ggcagcaacc cgcgacccaa ggcagaaaca
     5941 cacgtaagtc attccggctg gtgcctcgcg atctccatag aatcaagcat tgtacggcga
     6001 acacgggcaa cgccgtcaaa tgatgactga tggccagacc gcccaacaag ccgaagctca
     6061 cggcggttcc gcgacttact tcgcgttcgg acgtcactaa taggttcacc gcgcagaggc
     6121 tcattccagc tacgagggcc gcgtgaagcg catacacttc ggccaccaaa gcaaccccga
     6181 gcacggtcct cgataaacag aataggccgc ccgcacagac gcccgctaga tcgcgtgcag
     6241 catcgttgac gtcaggcgcg atggctgcca atactttacg aacaattgca cacacgagca
     6301 ggcccgcgat ggtgcagaac actgcactcg acgcatgcgc gacattaatt ggcgctgcgc
     6361 cggcccagaa ccagcaggcc gcgcgtacca gcatcaggta aagtggatat ccgggtggat
     6421 gagcggttcc ggaggtaatg gcagcggtga ccagctctgc gccgtcttgg gcgcggttct
     6481 tgagggtcag tccattcgcc aaactcgagg cgtaaatcca gggcactacg gcgagcaggg
     6541 ccacgaacgc ccgcgtcggt ttcagcctcg cagccgcgga caagagcagg cgaacaccct
     6601 cggagcgagc cccgcgcggc gtcccttgcg gtccagtccg cgctggcgtg atccgcaacc
     6661 ctgccgtggc gccgttcgcg tagcgagcct cgagccaagg taccgccaca gacagcccga
     6721 tccccagagt ggcgccggct atgacgtcca ctgcatagtg gaatcttagg tagagagtgg
     6781 cgaagatcag tgatgtaacg ggaatgaggt aaaaccagaa cagccaccga cactgccgat
     6841 aggcaaagat caatacgatg atcccccagg cggtatgcaa agaaggaaag cagttgggca
     6901 ccacgctcga cgcgatctgg gcgggatgac tcacgtgatc cgtcaaagcg gaaaagctgc
     6961 ccccttcaac gccccgcccc ggaaagagtt cgggaaagaa gtagcgaggg cccaccaccg
     7021 gaaccgccac gtaacccaga tatcccatcg ctcccgccag cacgaacgtc cgaaggaact
     7081 gccgaaactc gcgcattcgc ccggacaggt acaatacccc gccactgatc agcggtagag
     7141 caaagagcgc caagtagcac gcggagaacc actcggttct ggtgcgcgac gctagcggca
     7201 ccagccacac gctcggcgtc gtacccaaca tatagagatc ccaacgcaac atctgcgcgt
     7261 cgtacagatg aggacggaag actcggatca agggcagcaa gttctcgtaa actgcaatca
     7321 gcaccacgaa aggaccccaa tcccgtaaga actgcagggc ccgctggtat ggcctgcatt
     7381 tggctccgac agaacgagaa ccgccgagaa catagaatgt cgcgataaac gcaaagatcg
     7441 ccgctccgcg cgtcgtaaag taggtgttcc aacggattgt cgcactcaga aggatgcgac
     7501 agcagagcag caccagcacg gacgccaccg ccccaacttc ctctgctcgc cagcctccaa
     7561 cgaaccgacg gatccgagtc gctccgaatg tcatggcgta gttaattcga agtcactaag
     7621 gcgttacgga aggcccaggg cagccgctcg tgtcccagct cgtgctcgtg tcccagctcg
     7681 tgctcgtgtc ggccccctcg ttcaggttgc taccccaact ggtgctcgca tcctggccat
     7741 ctgaccgaga tgcacacccg ggcagcagcg tatggatcac ggcggcaacc agaaaacccg
     7801 atacagtaca aatcgaatac ttcacactac acctccaagt tctgaaccga tccgcggcga
     7861 agcgcgcggc tctcgtccag ggccgcgtgc tcgcgcgatc ggctcaccca acaaaaagaa
     7921 tctaagcgtg gcccgatcgt ctttgctcga ggccctatag aactcctctc ccccgctcca
     7981 tccgtgcaag ccactcgtac attcatcgat gcccccctcg gctcgccccg ctccgaagca
     8041 gcggcggagg aggatacgga tctggtgaag gaggcgctcg cgggcaaccg ctgggccgag
     8101 gaagcgctct actatcgaca cgttcgtggc gtcactcggg tagtgcttcg cctcctgtcg
     8161 agatcgtccg aagtagaaga tgtcgtgcag gataccttcg tgatcgcctt tggagaccta
     8221 gcggtactcg cggatcccgc cgcgttccga cattggctaa tcagaattgc agtccggcaa
     8281 gtgcaccgtc ggtttcgtcg aaggaagctc ttgcgtctcc tcggcctcga cgccagtcaa
     8341 gacgaccaga tcctggcggc ccaagccgat cccaaggcgg gaccagagac ccgcgtccta
     8401 ttaggggagg tcgaccgaat cttggccaaa ctcccaatcc gcgcccgcgt ggcgtgggtg
     8461 cttcgaatgt ggagggacag aaactcgagg acgtagcgtt tgcgtgtggc tgctcgctct
     8521 cgaccacgaa gcgtttactc agccgggccg gctcggccct gtccgaatac gcgagcttcg
     8581 aagcatcgga ggaatcatga aacagctacg gcctgttgtg cccaagcgcc gtcgcctgcc
     8641 tttgccggtt tctcgagtgc tcgcggaccc gctcgatgag cgggccatca acaagatgtg
     8701 gcgcacgatt gagcgacgac gcgggcagcc ggtgcatccc atcggcaagt ggatcttggt
     8761 tttggcatgc atcggatcgc tgggcgtgtt ggcttcagtg ctgatgccga ggaatcagcc
     8821 gcagcccctg gcggcagcca ataacgtcgg cgcaaaagcc aactcttccg ctcaagcttc
     8881 gctgctctcc ccgatcgaag tgccgcccat ggcacctccc gtcgacgtca cgatggctga
     8941 cggttcacac att
//
LOCUS       DASTKU010000632         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1908136, whole genome shotgun sequence.
ACCESSION   DASTKU010000632 DASTKU010000000
VERSION     DASTKU010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908136"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VFQ61_22055"
     CDS             <1..784
                     /locus_tag="VFQ61_22055"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9957204.1"
                     /translation="NALVRFAVLGDYGFAGPAEAQVAELIKGWKPEFVLTTGDNNYPD
                     GSAETIDANIGQFFREFICPYRGKYGAGSKVNRFFPALGNHDWYTAGARPYLDYFTLP
                     GNERYYDLLWGSVHVFVIDSDPNEPDGVESGSRQAKWLEDGLRSSKARWQLVTMHHPP
                     FSSGPHMSNAYMQWPYKEWGADLVLGGHDHDYERFERDGLTCVVIGLGGASIYEFGTP
                     AEGSSARFNANYGAALVEADSEKLVLRFYAAPGQLVDTFTLK"
     gene            872..>2469
                     /gene="murJ"
                     /locus_tag="VFQ61_22060"
     CDS             872..>2469
                     /gene="murJ"
                     /locus_tag="VFQ61_22060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET9957205.1"
                     /translation="MSESPRPPPAKVVPAEIKAASDVSGASETSPDAPTNSPTPSREG
                     MTHASLPPSPSTASDREAAVERGRLARRASVVGAGTLGSRVLGLVRDQVMAAVFERGI
                     TDAFFVAFTLPNVLRQVLAEGAVQSAVLPVLSQTRERDGDPAARSFFAAMRGVSLTLL
                     AVVSVLGVLLAPQLVTLFAGGFRDLPGQFERTVELTRWVFPYIFCMGTAALGMAALNT
                     YQRFAVAAFAPALLNLAFIACSFGLPGTLRERGLDPALALAFAVLIGGVLQVVAQWPS
                     LRAIGFLNRPSLDLNHPGVRDVARRMVPVLFGMGVYYVDVLLARRLLSGLGLGAQSYF
                     SWALRLCDFPQGIFVMALQTAALPNLSRLAARGDTAEFSRTFAFGLRLALFVALPATA
                     VCVALAHPLVIVLFQRGAFDPESAHETARALVAQGSGIWLVAAVRQLVSAYYALGDTR
                     RPTQVAALDLVAFIVAALALRGPLGHVGVGAAVAISSGVQALLLWRGLRSRLLGSDRA
                     QIRRSILIHGSCSVLGGLCAAGFAS"
BASE COUNT          368 a          783 c          804 g          514 t
ORIGIN      
        1 gaatgcgttg gttcgctttg ccgtactcgg agattatggc ttcgcgggtc ccgcagaggc
       61 gcaggttgca gagctgatca agggctggaa accagagttc gttctgacta cgggcgacaa
      121 caactatccc gatggttctg ccgaaaccat cgacgccaac atcggtcaat ttttcaggga
      181 gttcatttgc ccataccggg gcaaatatgg cgcaggcagc aaagtcaatc gcttcttccc
      241 cgcgctcgga aaccacgact ggtacacagc aggcgctcgg ccctatttgg attacttcac
      301 acttcccgga aacgagcgtt attacgactt gctctggggg agtgtgcacg tgttcgttat
      361 cgacagcgac ccgaacgagc ccgatggcgt ggagtcgggc tcgcgccagg ccaagtggct
      421 ggaagatggg ctgcgctctt ccaaagcgcg ctggcagctc gtaacgatgc accacccgcc
      481 cttctcgtcc ggcccgcata tgtcgaacgc gtacatgcag tggccataca aggagtgggg
      541 cgcggacctg gttctcggcg gtcacgacca tgactatgaa cgcttcgagc gggacggtct
      601 gacctgcgtt gtcataggcc tgggcggcgc gtccatttat gagttcggca cgccagccga
      661 gggcagctcg gcgcgtttca acgccaacta cggtgccgct ctggtcgaag ccgacagtga
      721 aaagctcgtg ctgcgtttct acgctgcgcc cggccaattg gtggacacct tcacgttgaa
      781 atgatcgcga gccacccagc gcggtgggcg cgaccgggtg gaggacgcga gtcattgggc
      841 acttcgtcgt agatcagcta agacgcgcgg cttgagcgaa tctccgcgcc cacctcccgc
      901 taaagtcgtt cccgctgaga tcaaggcggc ctcggacgtg tccggggctt cggagacgag
      961 cccggatgcg cccacgaatt cacccacgcc atcgcgggag ggcatgaccc atgcgagcct
     1021 ccccccgagc ccgagcacgg cgtccgatcg cgaagctgcg gtcgaacgag gccggttggc
     1081 gcgtcgagct tccgtcgttg gcgccggaac cttgggcagc cgtgtcctcg gcttggtgcg
     1141 agatcaagtc atggccgcgg tcttcgagcg cggcatcacc gacgcattct tcgtagcctt
     1201 cacgctcccc aacgtgctgc gccaagtgct cgccgaggga gcggtgcaga gcgccgtgct
     1261 gccggtgctc tcgcagaccc gcgagcgcga tggtgatcca gctgcgcgtt ctttctttgc
     1321 ggccatgcgc ggcgtctcgc tgacgctgct cgcggtggtg agcgtgctgg gcgtgctact
     1381 cgcgccgcag ctcgtgaccc tattcgcggg cggctttcga gatctgccgg gtcaattcga
     1441 gcgcacggtg gagctcaccc gctgggtctt tccttatatc ttctgtatgg gcacggcggc
     1501 gctgggcatg gccgccttga acacctatca gcgcttcgcc gtagcggcat tcgcccctgc
     1561 cctgctcaat ttggctttca tcgcttgctc cttcggtttg ccgggaacgc ttcgggagcg
     1621 gggcctggat cccgctctcg cgctggcgtt tgcggtgctg atcggcggcg ttctgcaggt
     1681 cgtagcgcaa tggcccagcc ttcgcgcgat cggttttttg aaccggccca gtctcgacct
     1741 gaaccacccc ggcgtgcgcg acgtggcccg acgcatggtg ccggtgctgt tcggcatggg
     1801 ggtctactac gtcgacgtgt tgctggcgcg gcggctgttg tcagggctcg gtctgggagc
     1861 ccagagctac ttcagctggg ctctacgtct gtgcgacttt ccacaaggca tcttcgtcat
     1921 ggccttgcaa accgccgcgc ttccaaacct gtcgcgtcta gcggcccgcg gggacacggc
     1981 ggagttctcg cgcaccttcg cgttcgggct acgactcgcg ttgttcgtcg cgctgccggc
     2041 taccgcggtg tgtgttgcgc tcgcgcaccc gctcgtgatt gtgctattcc aacgcggggc
     2101 attcgatccc gagagcgcgc acgagactgc gcgagcccta gtcgcgcagg gtagcgggat
     2161 ttggctggtc gccgccgtac gccagctcgt gtcggcttac tacgctttgg gtgatacccg
     2221 gcggcccacc caggtcgcgg cgctggacct cgttgcgttc atcgtcgcgg ctttggcttt
     2281 gcgtggaccg ctgggccacg tgggggtcgg cgccgccgtc gccatctcga gcggcgttca
     2341 ggcgcttctg ctctggcgcg gactgcgatc acgactgctg ggcagcgatc gcgcgcagat
     2401 caggcgatcg attctgatcc atggcagttg ttccgtactc ggcggcttgt gcgcagcagg
     2461 tttcgcctc
//
LOCUS       DASTKU010000633         3538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1461775, whole genome shotgun sequence.
ACCESSION   DASTKU010000633 DASTKU010000000
VERSION     DASTKU010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461775"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFQ61_22065"
     CDS             complement(<1..555)
                     /locus_tag="VFQ61_22065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957206.1"
                     /translation="MSNSPHLARMHRRLFLRGAGGAVLALPVLPSLLHPRAARAEASA
                     APKCFVHFRTPHGGVSTSNMWPADSALTETSSYTHEIRRGALSANVSNGTSSVSPVLS
                     ASSGVLTPALLAKMNILRGLDIPTAMAHNFGAALGYYDVDHQTPARPTATVDQVLAYS
                     PAFYPNLSSVKQRSVVISAPGTNSG"
     gene            complement(552..2468)
                     /locus_tag="VFQ61_22070"
     CDS             complement(552..2468)
                     /locus_tag="VFQ61_22070"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9957207.1"
                     /translation="MLRSCLLSLAGLIGACTTATGDPTASDGPTDTTHTGSMGGSGTG
                     GEQTGGAPSSSGAQGGGAQGGGAQGSGAQGSGAQGSGGTAAGGQGPTTSAAPLFSCDP
                     SAKAGELPFPRLSRVQLENTLKFAVRLALPTDADKIWSVVAPRFAQYPLDQRTAAPGD
                     LKGGYSRFDQSIQLAQAQAMYATGLELSRELTASSTRMKSLLGACATDASTANDRSCL
                     EDFVRRWATRVMRTELSEEDITFYAEMSDGTPVSAAAVADVIAAILNAPETLYRVEHG
                     TDANSPSALSAYELAARLSYNLWQEPPDDELWATAANGSLLQPSTYQVQLERLIEDSK
                     LRRSLDEWVSEWLRLGELPRLDTLKSDPRFLAFAGSELPNESTRQAMIDDVLESAWST
                     VRAGESASALLNDRHSYAKDSILARIYGVPVWTGAGDPPLMSATSRSGLLTRAALLAT
                     GTAGTRPIHKGYLVRNALLCQQLGAPPLNANTEVPVANGQLTTREAASQRTSPAACAG
                     CHAVINPAGFVTEGFDALGRERSEEQLFDAQGELIASLPLDTAATASILPGDTRVLRS
                     ASELSEAIDDSRLFHSCLARHYFRFAAARVENTQTDGCLLAKLESAARSDAPLTAVLR
                     AFAQDATFKTKRFE"
     gene            2604..3245
                     /locus_tag="VFQ61_22075"
     CDS             2604..3245
                     /locus_tag="VFQ61_22075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9957208.1"
                     /translation="MATWVRPLCRTPAELDEALTRERTSALSEATRLRECDPAAPRPL
                     DLESVYASQREFVWLTLQRMGVRRSDLEDVFQDVFMIVHRRLHTYRADAKLSAWLYGI
                     CLRCAARHRRRAFHRREHPADVEPEAGLWPSSMTAPDQRLDARQRQERLNQLLDVLDA
                     EHRSMVVMYEIEELSCAQIAELTGVKLGTIHSRLHTARRKLAAAAQRLKGDSR"
     gene            3242..>3538
                     /locus_tag="VFQ61_22080"
     CDS             3242..>3538
                     /locus_tag="VFQ61_22080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957209.1"
                     /translation="MNDPIRIREPGSDASSELRELFRDAQRPQPLTPTIQADLASRVA
                     SIAATRGTLLTAVTAKAPIFLVGAALLGASMVALREPTPKTEPVAPVEPVSTGSS"
BASE COUNT          631 a         1108 c         1163 g          636 t
ORIGIN      
        1 gccgctattc gttccgggag ccgaaatgac gacgctccgt tgtttgaccg agctcaggtt
       61 tgggtaaaac gcgggagagt acgcgagcac ctgatcgacg gtggcggttg gtcgagcggg
      121 cgtttggtga tcgacatcgt aatagccgag tgctgcgcca aagttgtgcg ccatggcggt
      181 cggaatatcc aggccgcgca agatattcat cttggccagc agagcgggcg tgagcactcc
      241 ggagctagcg ctgagtaccg ggctgacgga gctggtgccg ttcgacacgt tggcggaaag
      301 ggcgccgcgc cggatctcgt gagtgtacga cgacgtctcg gtcagcgcgc tgtcagcagg
      361 ccacatgttg gaggtggaga cgccaccgtg cggggtccga aagtggacga agcacttcgg
      421 tgcggcgctg gcttccgcac gcgccgctct cgggtgaagg agcgagggca ggacgggcag
      481 cgcgagcact gcgcccccgg ccccgcgcag aaacagccga cgatgcatgc gagccaagtg
      541 cggactattg ctcattcgaa cctcttggtc ttgaacgtgg cgtcttgggc aaaagctcgt
      601 aggactgcgg tgagcggcgc atcgctgcgg gccgcgcttt ccagcttggc gaggaggcaa
      661 ccatcggtct gggtgttctc cacccgcgct gccgcgaagc ggaagtagtg gcgagccaga
      721 cacgagtgaa acaagcggct gtcgtcgatg gcttcgctca gctccgacgc ggagcggagg
      781 acccgagtat cacccggtaa gatcgaggcc gtggcggcgg tatcgagcgg caaagaagcg
      841 atcaactcgc cctgtgcgtc gaacagttgc tcctcgctgc gctcgcgccc cagcgcgtcg
      901 aagccttcag tgacgaagcc ggcaggattg atcacggcgt ggcagccggc gcaggcggcg
      961 ggtgacgtcc gctgtgaagc ggcttctcga gtcgtgagct gcccgttcgc gactggcacc
     1021 tcggtgttgg cattgagcgg tggggcgccg agctgctggc ataacagcgc atttcgcacc
     1081 agataaccct tgtggatagg tcgagtgcca gccgtaccgg tcgccaatag ggcagcgcgg
     1141 gtgagtaagc cgctgcggct cgtggcgctc atcagcggcg ggtccccagc gcccgtccaa
     1201 actggaacgc cgtagatgcg ggccaggatc gaatccttgg cgtacgagtg tcggtcgttg
     1261 agcagcgcgc tcgctgattc tcccgcacgc accgtgctcc aagcggactc gagcacgtcg
     1321 tcgatcatgg cctggcgcgt cgactcgttg ggcaactccg atccggcaaa cgccaggaag
     1381 cgcggatcag acttcaaggt atcgagtcgc gggagctctc ccagtcggag ccactcactc
     1441 acccactcat cgagcgaacg tctcaatttg gagtcttcga tcaggcgttc gagctgaacc
     1501 tggtacgtcg agggctgtag caacgacccg ttcgccgcgg tcgcccacag ttcgtcatcg
     1561 ggcggctcct gccaaagatt gtaggagagc cgcgccgcga gctcgtaggc ggagagtgcg
     1621 ctcggtgagt tggcgtcggt gccgtgctcg acgcggtaga gggtctctgg tgcgttgagg
     1681 attgcggcaa tcacgtcggc gaccgccgcg gcgctcaccg gcgttccgtc gctcatctct
     1741 gcgtagaagg tgatgtcttc ctcgctcaac tcggtccgca tcacgcgcgt ggcccaacgg
     1801 cgcacgaagt cttctaggca actgcggtcg ttggctgtcg aggcatccgt cgcgcaggct
     1861 cctagcagcg acttcattcg agtggatgat gcagtcagct cgcgcgagag ttcgaggccc
     1921 gtcgcataca tcgcctgtgc ctgcgccagt tgaatcgact gatcgaagcg ggagtagccc
     1981 cctttcagat cccctggggc ggccgttcgc tggtcgagcg gatactgcgc gaagcgaggg
     2041 gcgacgacgc tccaaatctt gtcggcatcg gtcggcagcg ccaaacgcac ggcgaacttc
     2101 aacgtgttct ccagctgcac gcgagatagt cgcggaaacg gcagttcgcc ggctttggcc
     2161 gaaggatcac aggaaaatag gggtgcggcg gacgtagttg gcccttgacc accagctgca
     2221 gtccccccgc taccttgagc accgctgcct tgagcgccgc tgccttgagc accgccacct
     2281 tgagcgccgc caccttgagc gccactgctg gagggagcac cgccggtttg ttctccaccg
     2341 gtaccactcc cacccatgct accagtgtgg gttgtatccg ttggcccatc ggaggcggtc
     2401 gggtcgcccg ttgcggtggt gcaggcgccg atcaaacccg cgagtgagag caagcatgaa
     2461 cgcagcattc ttcctggtaa tgctggcttc tcgccgttag cttcagacaa agtgcacggg
     2521 ccattcgacc cgatcacggg gtgcctcggc actgggcgct ccccgaaccc gagcatgcga
     2581 accttctcgc gtgtaagatc cgcgtggcta cctgggttcg cccgctctgc cggacacccg
     2641 ccgaattgga tgaagcattg acccgcgaac ggacatcagc gctgagtgag gcaactagac
     2701 tacgcgagtg tgaccccgca gcgccgcgcc ccctcgatct cgagtcggtc tacgccagcc
     2761 aacgcgagtt cgtttggctc accctgcagc ggatgggggt gcggcgctcg gacttggaag
     2821 acgtttttca ggacgtattc atgatcgtgc atcgccggct ccacacctac cgcgccgacg
     2881 ccaagctttc agcgtggctc tatgggattt gcttgcgctg cgcggctcgg caccgacgcc
     2941 gtgccttcca ccgacgcgag cacccggcgg acgtcgagcc cgaagccggt ctttggccgt
     3001 cgagcatgac cgcacccgac caacgattgg atgcacgcca gagacaagaa cgcttgaacc
     3061 agctgctgga cgtgctcgat gcggagcatc gctcgatggt cgtgatgtac gaaatcgagg
     3121 agctgagttg cgctcaaatc gcggagctca ccggggtcaa actcggaacc atacactccc
     3181 gtctacacac cgcgcgcaga aaattggcgg ccgcagcgca acgtctaaag ggggatagcc
     3241 gatgaacgat cccattcgca tccgtgagcc gggctccgat gcttctagcg agttgcgtga
     3301 gctgttccgc gacgcgcaga gaccccagcc gctcactccg acgatccagg cggacctcgc
     3361 ttcgcgcgtc gccagtatcg cggccacacg gggtacgctg ctaacggctg tgaccgcaaa
     3421 ggcgcccatc tttctcgtcg gcgccgcgtt gctaggggcg agcatggtcg cgctacgcga
     3481 acccacgccg aagaccgagc cggttgctcc cgtcgagccc gtatcgactg gttcgagc
//
LOCUS       DASTKU010000634         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_122558, whole genome shotgun sequence.
ACCESSION   DASTKU010000634 DASTKU010000000
VERSION     DASTKU010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122558"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2177)
                     /gene="xdhB"
                     /locus_tag="VFQ61_22085"
     CDS             complement(<1..2177)
                     /gene="xdhB"
                     /locus_tag="VFQ61_22085"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HET9957210.1"
                     /translation="MTVPAPPSAAEPIDAGAPAEQLAPAQQLAPAQQLAPAEQVRPAE
                     QAGPVGKAVSHESATLHVTGRAQYVEDLATRAAHLVHAWPVLAPLDHARVLEIDACDA
                     LQCPGVVGWLSAADVVGENDVGPVRHDEPLFPSEVCFHGQPVAWVLGESSEQARLAAE
                     RVLVRYEALPAVRTLLQAIAEDSYLAPAEHLERGDAESALQVAPRRIQGELFVNGQEH
                     FYLETQAALALGDDQEGILVHSSTQHPSETQEIVARVLGVPRHTVTVQCLRMGGAFGG
                     KETQANAWAAIAALATKKTGRPTLVRLTRQQDMRLTGKRHPALGRFRVGFDARGVVSA
                     LNLELFLDGGFSLDLSLPVLMRALFHADNCYRVPNLRVVGRVCRTHRVSQTAFRGFGG
                     PQGMLMIEEVLDRVARTLGLPPHVVRERNLYREGDRTHYGQIVKDADRITRIWQELKA
                     ASDFDARWSATQQFNASSLHYKRGLAITPVKFGISFTATFFNQAGALVLVYRDGSVQV
                     NHGGTEMGQGLHTKIQQIAAQVLGLPLTSVRMMATRTDKVPNTSATAASSGSDLNGAA
                     VKNACETLRERLGEVAGRRLGVPASDVVFENGRVFPWMTPKHAVSFAELAELAYLERT
                     PLSAAGYYKTPRIHFDRETRQGKPFHYFAYGAAVSEVEVNGFTGQYTLLRSDILHDVG
                     DSLSPLVDRGQIEGGFIQGIGWLTTEELVWSSDGEFLSNGAST"
     gene            complement(2174..>2779)
                     /locus_tag="VFQ61_22090"
     CDS             complement(2174..>2779)
                     /locus_tag="VFQ61_22090"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET9957211.1"
                     /translation="FSSRAIRNRATLGGNLATASPIGDSAPVLLALGAELVLRKRGSE
                     RRLPLSEFFTGYRRTSLEHGELIVAVRIPAPFPERQRFYKVSKRVCDDISTVSAAFGL
                     SLDSSGRVRSLTAAFGGVAATPLLAMSLTGAAVGRPWDAATLALLLGEVGSLGTPQSD
                     VRGSAEYRRHMMGQLLRQFFYETEGPATLTRVAANDTQGAQ"
BASE COUNT          534 a          928 c          838 g          479 t
ORIGIN      
        1 taggtcgagg caccgttcga gaggaactcg ccgtctgaac tccacacgag ctcttcggtc
       61 gtgagccagc cgatgccttg aatgaagccc ccctcgattt gtccccggtc gaccagggga
      121 ctcagcgagt cccctacgtc gtgcaggatg tccgaacgaa gtaaggtgta ctgtcccgta
      181 aagccattga cctccacctc gctcaccgcc gcgccgtaag caaagtaatg gaatggctta
      241 ccttgacggg tctcgcggtc gaagtgaatg cgaggcgtct tgtagtagcc ggccgcagac
      301 agcggcgttc gctccagata ggcgagctcc gcgagctccg cgaacgagac ggcgtgtttc
      361 ggcgtcatcc acgggaatac ccgcccgttt tcgaaaacga cgtccgaagc cggcactccc
      421 agacggcgcc ccgcgacctc gccgagccgc tcccgcaacg tctcgcaggc gttcttcacc
      481 gctgcgccat tcaaatccga gccgctcgac gccgccgttg ccgaggtatt cggaaccttg
      541 tcggtgcgcg tcgccatcat gcgaacactc gtcagcggta gccccaacac ttgggccgcg
      601 atttgctgaa tcttggtatg aagcccctgc cccatttccg tgccaccgtg gttcacctgc
      661 acactgccat cgcgatatac gagcaccagg gcgccggcct gattgaagaa ggtcgcggtg
      721 aaggaaatac cgaatttaac cggggtgatc gctaggccac gtttataatg caggctcgaa
      781 gcattgaact gctgcgtggc agaccagcgt gcatcgaaat cgctcgcggc cttgagttct
      841 tgccaaatgc gagtaatccg gtcggcatct ttgacgatct gcccgtaatg ggtgcggtct
      901 ccttcgcgat aaagattacg ctcgcgcacc acgtgcggtg gtaaaccgag ggtgcgggcc
      961 actcgatcga ggacctcctc gatcatcagc attccctgcg gcccgccaaa cccgcggaat
     1021 gcggtctggg aaacgcggtg cgtgcggcac acgcgcccca cgacccgcaa attgggaacg
     1081 cgatagcaat tgtctgcgtg aaacagcgct cgcatcagca cgggtaggct caagtccaag
     1141 ctgaagccac cgtctaggaa gagttctagg ttcaaagcgc tcaccacgcc gcgagcgtcg
     1201 aagccaacgc gaaagcgacc gagcgccggg tggcgtttcc cggtaagccg catatcttgc
     1261 tgtcgcgtca agcgcacgag cgtcgggcgc cccgtcttct tggtggccag cgctgcgatt
     1321 gctgcccaag cgttagcttg ggtttctttg cctccgaagg cgccgcccat gcgcaagcac
     1381 tgcacggtta cggtgtgccg gggtacaccg agcacacgtg cgacgatctc ctgagtctcg
     1441 gacggatgct gagtcgaaga gtggaccagt atgccttcct ggtcgtcgcc gagcgctaga
     1501 gccgcttggg tttccaggta aaagtgctcc tgtccgttga cgaagagctc cccttggatc
     1561 cgccgcggag cgacctggag agcactctct gcgtcacccc gttcgaggtg ctcggcaggc
     1621 gccaagtagc tgtcctcggc gatggcttgc agcaacgtgc gcaccgccgg caatgcttcg
     1681 taacgaacca gcacccgctc cgccgcgagc cgggcctgct ccgagctttc cccaagcacc
     1741 catgccaccg gctgcccatg aaaacagact tcgctaggaa agagcggttc gtcatggcga
     1801 acgggcccga cgtcgttctc cccgactacg tctgccgcgc tgagccaacc caccactccc
     1861 gggcactgca gggcgtcgca agcgtcgatc tcgagcacgc gggcgtggtc gagaggggcc
     1921 agcaccggcc aggcatgaac gagatgagcc gcgcgcgtcg cgagatcttc cacgtattgg
     1981 gcgcgaccag tgacgtgcag cgtggccgac tcatgactga cagccttgcc cacaggaccg
     2041 gcctgctccg ctggcctaac ctgctccgca ggtgcaagct gctgcgcagg tgcaagctgc
     2101 tgcgcaggtg caagctgctc cgcaggtgca cccgcatcga tgggctctgc ggcagagggc
     2161 ggggcgggca cagtcattgt gccccctgag tgtcattggc cgccactcga gtcagtgtcg
     2221 cgggcccctc cgtctcgtaa aaaaactgac gaagcagctg ccccatcata tgacgacgat
     2281 actcggcgct gcccctcacg tcactctgcg gagtacccaa cgaacccacc tctcccagca
     2341 acagggcgag cgtggccgca tcccagggtc gccccaccgc ggcgcccgtg aggctcatcg
     2401 ccagcagcgg cgtcgccgcc acgccaccga aggcggctgt cagcgaacgc acccgcccac
     2461 tcgagtccag actcaagcca aaggctgcag aaaccgtgga gatgtcgtca cacacccgct
     2521 tgctgacttt gtagaatctc tgccgctcgg ggaaaggcgc tgggatccgc accgcgacga
     2581 tcagctcgcc gtgctccaag gacgtgcgcc ggtaacccgt gaagaactcg gataggggca
     2641 aacgacgctc gctcccgcgc tttcgcaaca ccagctccgc gccgagcgcg agcaacacgg
     2701 gcgccgaatc tccgatcggc gatgccgtgg ccagattgcc gccgagcgta gcccgattgc
     2761 ggatggctcg cgaagaaaa
//
LOCUS       DASTKU010000635        25040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_122559, whole genome shotgun sequence.
ACCESSION   DASTKU010000635 DASTKU010000000
VERSION     DASTKU010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 25040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25040
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122559"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VFQ61_22095"
     CDS             complement(<1..1067)
                     /locus_tag="VFQ61_22095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957212.1"
                     /translation="MAGGARGCFWLLLLASCSLPEFRFREAKPEGNCSDGLLNGSETD
                     VDCGGQCLACAVGRFCSKNVDCEQQSCVDRRCQQDHCTNQRLDVSETDVDCGGGACPA
                     CGVSQRCRAGSDCSSNACVEARCVATGCDDGSRGDDETDLDCGGGTCPACAYGRHCAL
                     GGDCETGICLSSACSSPSCADGTQGEQETDVDCGGLMCPRCLVGQACKGSGDCDSGVC
                     EQGRCVAQACGSALACAGGGGSGATAGAPTTGGLSTAGGSSSSAGQAAQSGASTTGGI
                     ITGGGEGVGGTQGNSESGGATSASGGASNNNGGSPSPNGGAGPSGGTSTAPSSGGTSA
                     SGSGNSPSGGTLAASGGTSAAT"
     gene            complement(1471..1860)
                     /locus_tag="VFQ61_22100"
     CDS             complement(1471..1860)
                     /locus_tag="VFQ61_22100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957213.1"
                     /translation="MSFGPRQIFQRAAALIALSVAITGASRIAAADPDEDAERARIAR
                     ELEDTRQRVRMLEERLRRLDSRRRDGAQSYESGVAARCSLPFFLDSSGVKRLRPECAA
                     EAPSRCDYPFSVGYDGIKRFRSSCGRN"
     gene            2162..3670
                     /locus_tag="VFQ61_22105"
     CDS             2162..3670
                     /locus_tag="VFQ61_22105"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9957214.1"
                     /translation="MRSLSVGQRILGGFAAVLVVTALLGAFVLLRLRVVDEEFKRSAQ
                     QSVPAIVLAGQIRAIIKEDQIGILRYLLTPATNLDEHRIIEDEARQRSAEVTRGLAEL
                     RKLLSDPEQLQMHDEVLKARENYLSVRRELFVNSRNNAVERESLLPRALPAYQAYIAQ
                     LNRIVEQCSKGSARSLSAVSDATESTRYGVWIGLGIALLCGGAIAFQIVLSTTGAIRA
                     VAGRLGRSSAHVASAASHLKASSHTFAGGASEQAASLEETSASIEEISSTIAQNADSA
                     VRAKQLSDEARSAAEQGANDIESMTAAMEAIRISSNNVTKIIKTIDEIAFQTNILALN
                     AAVEAARAGEAGAGFAVVAEEVRSLAQRSASAARETAAKIADAIDKSEQGAEVSTRVS
                     ASLGSIVTKVRNVNSLIGEIASASQEQSKGISQVSTAVAQMDRVTQNNAAGAQESASA
                     AEELSSQAALLDAAVGELEELFGESHSRSLGSDAEIRTMPQRGSRARMRLAS"
     gene            complement(3735..4355)
                     /locus_tag="VFQ61_22110"
     CDS             complement(3735..4355)
                     /locus_tag="VFQ61_22110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957215.1"
                     /translation="MHNAAAPIAELSTTDLHAVLPSTLDAGLLGPAAEALSEKPQRAR
                     SERVRFGGYAKPRRSVAHDRTPNGLQVREISDLEGIREANETSKPDASTQDSSMQSIN
                     QAGGQTRSPSVQAQAQVHFEQQGTQLTAIEIGRESRFPVVSALHRTLFALGIVVASYH
                     VRTRPTGLVERVELQRRDGGAIEGSLSAAIKEAVLPIALNASDRPV"
     gene            4761..6035
                     /locus_tag="VFQ61_22115"
     CDS             4761..6035
                     /locus_tag="VFQ61_22115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9957216.1"
                     /translation="MTASASQTHRTIRAIYGIEAPKLVARLARLLRDVGLAEELAQDA
                     LVAALENWPGSGIPENPGAWLMATAQRRAIDELRRGRRVEQKHDLLEQELALAQSLAE
                     PDGAADASVGDDLLRLMLISCHPVLPPEGRVALTLRLLGGLTTEEIARAFLVPEPTVA
                     QRIVRAKRTLAERRVPFDLPRGAELTERVGSVLEVLYLIFNEGYSATSGDTWLRPDLC
                     EEALRLGRVLAELAPQEPEVHGCIALLELQASRMAARVDAQGAPILLLDQDRRRWDML
                     MIRRGLAALARAERLGGVDGPYALQAAIAACHAHATRAEDTDWPKIAALYTRLAERVP
                     SPVVELNRAVAFGMAFGPQAGLHLVDELRKHPALERYHLLPSVRGDLLMKLARYREAR
                     EELERAASLTQNVRERALLLSRARECDKLNAK"
     gene            complement(6022..7260)
                     /locus_tag="VFQ61_22120"
     CDS             complement(6022..7260)
                     /locus_tag="VFQ61_22120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957217.1"
                     /translation="MRRSASTPGGGLIHPIALLSLVGLIVNDHYLKAAMPGVFTGKFS
                     DVCGLVLMPLWLQTAWELWPGRGLNPTRFRATSNRILGISVGVTAIAYSAVELWSPAE
                     FTYRYGLGAVQWPWFAITAVLERSPLPPLQPVRATADPSDLLALPALALAWYFGRSAA
                     GKAPPNELAPKARLASCKPITGLPLLEPGLLARRLLELGSTIVCVLWATPAWAAPPQS
                     RPSDRRHDGLFIEGGLNVGVLRLASNASISNGFQQRLRSTTWAPIFPSLTLAIGGTVA
                     PGLVLAGEYERSSASPLWISTRNDEFQMPDLALRVQQFAAFVRYYPEPTEGLYFGGAL
                     GLIQMSVQRRSEGFGGGGLPYGHELTGIAVTPEVGIGIWLSRGYSLSGAIRPQYAHLG
                     SDHLSAESLGLTLSAKLTWH"
     gene            complement(7257..7892)
                     /locus_tag="VFQ61_22125"
     CDS             complement(7257..7892)
                     /locus_tag="VFQ61_22125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957218.1"
                     /translation="MKTSFGEVWRCIRLIIALSSLFLVGCSGRQACFEGESSVLTEKT
                     SERTMALTRAGETVRTVFDIELASLPELWPVDGAVSSAVFSMAGSLHYVDAPDPTVMR
                     ELPRMLIWGRVMADATGDPSSSLPPPSSGTASVSWSTPLFGSCANTWNPGCCPFGARE
                     CKARVEFIVQRADGLPFPEVVLDWHGAATAQLNQCPEPFEGDLLISEVEGP"
     gene            complement(7889..8488)
                     /locus_tag="VFQ61_22130"
     CDS             complement(7889..8488)
                     /locus_tag="VFQ61_22130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957219.1"
                     /translation="MAFEKHSRADAKTLVQRWLIGLAFSASSLALLATSPPEPSSYYS
                     GERRLESIPIELDASHPTTAFEIEVNQLAAFPDGQWPRSISVRVQAVFGAEAEARWVL
                     ASIARLEPPDPKPTQEAVLTRFEHSFEAPFAGQCNDPPSATSPCRTRFRIEFAREESG
                     AAEGTVYGQLEIRLSERVASNNSASETIELPISVQVTES"
     gene            9031..10305
                     /locus_tag="VFQ61_22135"
     CDS             9031..10305
                     /locus_tag="VFQ61_22135"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9957220.1"
                     /translation="MTAIDKPAKLPQVLRVPERARKVKPFLRRFGRFLLGLVAASILL
                     TLVLLVDGWRAFGHRAEGQRLERMQRSPQFRDGHFVNPQPLVNHLLLMFEGLLHVSPN
                     ASPSTQIPTVAVDPKTFVVPPVSGLRVTWLGHATTLIEIDGKHVLTDPVWSERASPFT
                     WIGPRRWYTPRISLAELPVLDAVVISHDHYDHLDYRTMLALNELISNRGWATRFVVPL
                     GIGAHLAYWGIPESRIVELDWWEKTSLGEAADDKLEIVCTPARHASGRMLLDNDAKLW
                     ASYALIGPAHRAYFSGDTGLFPALRDIGQRLGPFDLTLIEVGQYHGGWPDWHIGPEQA
                     LTAHQWLRGRVFVPIHWGLFALAYHGWTEPAERVIAAARTAQSVVILPMPGESVEPSV
                     PEAEARASRPRWWPKLPWQSAAEAPIVSTQIQ"
     gene            complement(10380..11336)
                     /locus_tag="VFQ61_22140"
     CDS             complement(10380..11336)
                     /locus_tag="VFQ61_22140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9957221.1"
                     /translation="MSQEAASLPSVCVVIGSNRPGRLKAAVESVLRNDYPRLSLVVVA
                     QGNAEGGGPSWAQRELGAQLSDARLRLVLDEGRGVSRARNIGMANDTSEIILFTDDDC
                     IASPDWVARHVARYRDEPSTDMVFGAVLPPPGFTGADGEVPAFEPQKIVAQGRVEGDL
                     VLGMGANMSAKRRVLEALGAFDEQIGPGSPLPSGEDIDLSLRAAARGMRVVVEPTASV
                     WHDGVRAHGPESRVLWSRDGIGLGAVTAKCLRSGQYRAALMLTKLVVKLNAEAAAAVI
                     SGRRPTGLRRATLFGRGALQGLASGWRLRGTPSTMQHRMSHD"
     gene            complement(11544..13319)
                     /locus_tag="VFQ61_22145"
     CDS             complement(11544..13319)
                     /locus_tag="VFQ61_22145"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HET9957222.1"
                     /translation="MKRTRKWVGIGSMLLLLLPAAWGCASSTDDGATARPRTPVPSQG
                     PGSSSYAGSNNGAAQPPANGAAQPPANGAQPQPSTAQGGNTSSPVLSASGGSFTFGPS
                     AGADEPAPPPNSGDKYEAVGTNPFVLAEYDPLSTFAADADTASYDIFRRDVQTSVLPQ
                     PESVRLEEYVNSFAYQYPAPESESEVPFSIHLAAAPNVQRAETLLLRVGIQGKLAPAD
                     PGTANLVFLIDVSGSMAEQDKLPLVKTVLTEALDVLKPTDKISIVTYAGATLLRLPPT
                     PVANKAEITAVIQGLEAGGSTAGGAGMQLAYDQARAGFIEGGINHVVMCTDGDFNVGL
                     SSDTDLLNLVVEKRKSGVTLTTLGFGSGNLNDSMMEKVSNAGNGMYSVISNADQAIQY
                     AHGRLLSTMIHIAKDMKIQVEFNANRVLAYRLLGYEDRAIADTDFRDDQVDAGEVGAG
                     HRVTALYEVVPKGAAVPAPSGAPALEDGAVYDGAIECAPEDIVLVKIRYKQPGALETD
                     AAAEVSSSLSASDVADSSEALDADFKWAVAVASFAEILKQSPYAEPAHLEEIAALIQS
                     ARGTAADRMEFETLFKRARALLPAP"
     gene            complement(13445..14920)
                     /locus_tag="VFQ61_22150"
     CDS             complement(13445..14920)
                     /locus_tag="VFQ61_22150"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HET9957223.1"
                     /translation="MSWRAFGLIALGLSSAACSPSGSVPAEAPPRAAAVPGVVGKSSV
                     EWLVTTGDEHLAKRAVTSAGTSEPAPSTAVEVRIDPAQAFQTMQGFGGAFNEHGWDAL
                     GVLDAAERAQVLRALFDPEQGLGFNYCRVPIGASDYAIDRYTLDETPGDFAMDQFSIA
                     RDRERLIPYIQAARSIRPDLKLWGSAWTPPTWMKTNQSFDSGAMKDDTLVYAAYALYL
                     ARFVEEYAREGLNISMLVPQNEPGQLTRYPSCDWTPNQYITFIRDHLGPTFRKRRLRT
                     DIYVGTVNRPEWDALSVLNDPEARSYVSGIALQWFGLSHAGPILSAYPQLTFMQSETD
                     CGNHPWEPEFQPETPQNDFTYAAYTFRKFRDYIQAGASSYMLWNMVLDEHGKNLDSQL
                     PWPQNSAIVVDRQTRQVTYTPLYWMTKHFSSLVRPGAQRIATLSSYADQIAFKNPDGT
                     LIVELLNEGNSPIDLQVQAGQRRHLVQLPAQSFATLVIPKT"
     gene            complement(14998..16131)
                     /locus_tag="VFQ61_22155"
     CDS             complement(14998..16131)
                     /locus_tag="VFQ61_22155"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HET9957224.1"
                     /translation="MFRPHLAVGVVCLAGTFACSAAVSESDDAEASSASEALRGHGRP
                     SAVFTSSNSAEGNSVLAFRRSPRGNLEMVGDYATGGLGTGMGLGSQGALAVSEEGRFL
                     VTVDAGSNEITSFAVHGAELSLRSHVSSNGTRPVSVAIQDHRVYVVNAGEPANVAGYR
                     LGPDGTLTPIPGSIQPLSATAAGPAQVGISPRGNVLVVTEKATSRISTFGIGRSGALT
                     PRGAFASAGTTPFGFTFTPQGDLIVSEAGTASTSSYDVHRSGQVTVISAAVPDLQRAP
                     CWATVSPDGHFAYIANAGSSSISGYHVDPNGGLTLLHPDGITGAAGQNSRPLDMAVVD
                     RDLYLLDAGNHGISHFRIAPDGNLNLSESELSLPATAAGLAAY"
     gene            complement(16320..17792)
                     /locus_tag="VFQ61_22160"
     CDS             complement(16320..17792)
                     /locus_tag="VFQ61_22160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957225.1"
                     /translation="MTRTIRRSGSALFLSGLSKPAFWAVALPLMWGAACSKSESAKTE
                     PGAEASAEASPRPPNGTSLSGNASASATVSAAALAELTTPRIGGSLVRVGTFQVEVAP
                     YQSGRIEALVLDASGKPLNAAAELAVTVQASGGKSERVNLDFAAPQARFVGQAAAGVE
                     LTSGPIGVELKVGEQALQGKLALAAALPEATLGGHMLAVGQYSAEVVLDAGGEIRARL
                     RDATGALLKAGADVSANVTTSLDKAARLGLTFDAESQCFSGKLEGSARFVPGPLALHV
                     KVGGLESVGALLDAGISARASHGGAMLALGSYGVELVAEGPKLRAYVFDKLGSPMLDA
                     NLKLRAFAGARAATQLDFDWDASCRCYVSVQAPKLNLRVVPLHVNLGGFYGALASLDG
                     AMRASASAKLAGDAALGAKANLGAGVQVDAKAPTLDVKSKLNAASGAAAKAGAAVKVT
                     APRIEIQPPKLNVNLGASAGTKAKASAGAKASAGFSLGGK"
     gene            18025..18888
                     /locus_tag="VFQ61_22165"
     CDS             18025..18888
                     /locus_tag="VFQ61_22165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9957226.1"
                     /translation="MRGVSLAGKHAIVTGASSGLGVETARALSQAGANVVLAVRDVAA
                     GERLAGTLVGAGVGRVTVSPLDLSDLASVNAFVERHRGGPLDLLVNNAGIMATPLGYT
                     RQGLELQLGTNHVGHVALTLGLLPTLEQSSGARVVTVSSALHSRGRGGRLLETLEQDL
                     RYERRKYVPFDAYGDSKLANVLFTLELARRLPPQVRAFSLHPGVIPTPLSRSLGVGGV
                     LFRTLGAPFMKSVAQGAATSVFAATAPELDLHSGAYLSDCAIAMPSREATDPELAERV
                     WRATEAFIKAR"
     gene            19122..20489
                     /locus_tag="VFQ61_22170"
     CDS             19122..20489
                     /locus_tag="VFQ61_22170"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HET9957227.1"
                     /translation="MSAFTFHVSPLSESQVYRTIEAAGSATLEQLHRAIVAAFNLDQE
                     PAHAFFLNGHAWDAEFGVWGCADQSQRASSETRIESLELVERKRILYLYDFDAEICLD
                     VRVTRIEKDASGPAQPALVGSAGELPPSYLEPSSDADEDEEEDEEQADAEAEAKRAEL
                     LAAAEPTLAALAERVRACIEFAEVRLQMQFDEMEEHFEDAAVSGAGEEGSPSGQHEAV
                     EEGEGGEPGEADEHDDEEEGPSAEEEAELAEELLTRFGTDAQRITEQLEPLVDADVFD
                     WLESVPQRLSDEGDAKRAFALAQRIVQLPWSESLRALLPRLAVRADRPDEALSLIEVL
                     LRDEPQDPEILLQIALAFDELDQGTSAEQYYREALRYAGSDMDLRSEIIEHLVELLRQ
                     LGRDDQANKVMQAEVLHTQRFSPLFGPSSPPSEPFRNLLPKVGRNEPCPCGSGKKFKK
                     CCGLN"
     gene            complement(20637..21503)
                     /gene="sigJ"
                     /locus_tag="VFQ61_22175"
     CDS             complement(20637..21503)
                     /gene="sigJ"
                     /locus_tag="VFQ61_22175"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HET9957228.1"
                     /translation="MSDGVFEEERPRLVALAYRLLGSAAEAEDVVQDAYIRYRDLDAA
                     EIRNPSALLTTMVTRMAINVLASARWRRERYEGPWLPEPVVTGMERDPGSISTAFLLL
                     LETLSPRERAVFVLAKVFDYNHAEISEFLGVDVTTSRQLLHRASEHIESRKPRFRYAP
                     AVHARLVQEFLIACSTGDPERLGRVLATDVVARTDHGGKAQAARRLIIGVDRVARFAL
                     GLARKTAESAARYHLLPINGGPGLVLLHGGIPVTAITFETDGERICEINIVRNPDKLR
                     PLHALASSMTAS"
     gene            complement(21500..22669)
                     /locus_tag="VFQ61_22180"
     CDS             complement(21500..22669)
                     /locus_tag="VFQ61_22180"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9957229.1"
                     /translation="MSRDDNVLVLGAGYAGLSCAFRLAARKPTLPVTVVDARDSFCER
                     IRLHQVALGYEARSPKLGPWLAQSGISFIHTRVLGIDAKSRTVLTHEGTLSYRHLVYA
                     LGSDQDPGVPGARLHAASIASESSARSLASELVALSRGARVVIVGGGLTGLEFAAELA
                     EMRPELEVRLVAREALGTGTLSRSGAAKVRARLLELGVHLHECVEVSEVAAGRVIGPQ
                     LTLPFDVCVWATGFAAPGLAKESGLPTCGANRLVVDATLRVPSYPEIYGVGDAAQPNV
                     DVGAPALMSCRLGMPAGLHAAENIAREREGHAPEPWRVQDMLRCMSLGRDAGLIQLHE
                     PNGELREFSVAGHAAAWLKEGICRGTFWTLEREAKQAAHRRGLQPSSKSTMAYAT"
     gene            22916..23785
                     /locus_tag="VFQ61_22185"
     CDS             22916..23785
                     /locus_tag="VFQ61_22185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957230.1"
                     /translation="MNREASRAWAHSSISLSRLLGTSTALIATLATGCQVETRSWPTE
                     RRDSSDILVRDLRLNSDQELEVLRGVSVLKGGLSLGGSVTDLSALEQLTHIAGDLRVD
                     AARLESLSGLERLHSVDGSLNIAGAELVNLEGIGGLSQVGGYFYVSGEHLRSLKGLFG
                     LRRVRGDLLLSGKCPALRDFSGLERLVEVDGDVYIENHHVVSLRGLDGLRDVRGGLYV
                     YADLTDLTGLGALESVGGNLVLESLALSGLHGLMGVRQIGGYLSFRYTGVTDLSVLER
                     LDRLTRVEFHGSQ"
     gene            23831..24040
                     /locus_tag="VFQ61_22190"
     CDS             23831..24040
                     /locus_tag="VFQ61_22190"
                     /inference="COORDINATES: protein motif:HMM:NF025614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HET9957231.1"
                     /translation="MPRSRKTRPAATEVLCPFCGEPEAIHIDTGGGPHQVYTEDCPVC
                     CRPRVVHVEPGDSPGEYNVWLEREE"
     gene            complement(24093..>25040)
                     /locus_tag="VFQ61_22195"
     CDS             complement(24093..>25040)
                     /locus_tag="VFQ61_22195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957232.1"
                     /translation="CGSGFVRCGQQCIDLRSDPKNCGGCNQACGENQVCTGGACKTSC
                     NTSQTDCSGVCRDLKTDPANCGACGTACPSAPNAKPACAAGKCGITCNSGFSLCGDRC
                     VDTTTDLQNCGECGTVCASKPNATVSCERSMCVTRCNEGLTSCDGACVDTRSSVAHCG
                     ACGKACPAGQVCADGVCGTLCEAPKTDCSGDCTNVANDPRNCGGCGKACAAPENGTAV
                     CREKRCEFTCQAGLSACADACVDTSRDLNNCGACNKACSAPENGKAECKEGTCGVRCD
                     AGYAQCGSACVDLLFDGNNCGDCGNKCAKKETCLLGFCL"
BASE COUNT         4449 a         7938 c         8005 g         4648 t
ORIGIN      
        1 gtcgcggcgg aggtgccgcc acttgctgcc agcgtgcccc cgctcgggct gttgccgctg
       61 ccggaagccg acgtacctcc cgagctcggt gcggtactcg tgccgccgct gggccccgcg
      121 cctccattcg ggctcgggct gccgccgttg ttgttggacg ccccgccgct ggcgctcgtg
      181 gctccacccg attcagagtt cccttgtgtg ccaccgacgc cctctcctcc gcccgtgatg
      241 atgcccccgg tggtcgacgc accgctctgg gcggcttgcc ccgcactaga gctcgaacca
      301 cccgcggtcg agagcccacc ggtcgtaggc gcaccggcgg tggcgccact tccgccgccg
      361 cccgcgcaag caagcgcgct accgcaagct tgcgcgacgc agcggccttg ttcgcaaacg
      421 ccgctgtcgc agtcccccga gcctttgcag gcctggccta ccaggcaacg tgggcacatc
      481 agaccgccgc aatcgacgtc ggtctcttgt tctccctgcg ttccatcggc gcagctaggg
      541 ctgctgcacg ctgaagacag gcaaatgccc gtctcgcagt cgccgcccaa agcgcagtgt
      601 cggccgtatg cgcaggccgg gcacgttcct ccgccgcagt cgagatcggt ctcatcgtca
      661 ccgcgtgagc catcgtcaca accggtggct acgcagcgcg cttctacaca ggcgttgctg
      721 ctgcagtcgg agccggcgcg gcaacgctga ctgactccgc acgccgggca ggctccgccg
      781 ccgcaatcca cgtcggtctc gctgacgtcc aagcgctgat tggtgcagtg atcttgctgg
      841 caacggcgat ccacgcagct ctgctgctcg caatctacgt tcttcgagca aaaacgtccg
      901 accgcgcacg ccaggcactg gccaccacaa tcgacgtccg tctcgcttcc gttcagcaaa
      961 ccgtcgctgc agttgccttc gggcttggct tcgcggaacc gaaactcggg tagtgagcag
     1021 gaggccaata gcagcagcca gaagcaaccc cgtgcgcctc ctgccatgag aggtcgtctt
     1081 ggtacggagc cacgttcatg ctcgagttcg cgattcgacg atgagcgctc cgtatccgca
     1141 cgcgagcgct tgcgggggcg ccggaccgac cttgggttgc gcccgatcgc ctccgtggaa
     1201 gccacgggcg gcacgcgcag cgaggcttcg tgcttcactc ggttaatgta accaagatat
     1261 cgaagcgcgg cgagtgctgt tcgtagtctg cggcttcccc tttgggcgat gctgaactac
     1321 gtaacagtgg attattaata ctctcgggtg attgctggta cccgatttcg atttgcacaa
     1381 gctgtgcaaa tcgggctgca ctcggcgcga cttcttcggg ctgcgggcgc gacagcttcg
     1441 cgagaggggc tgaagtctct gaccgtagag ctagttgcgg ccgcagctcg accgaaatcg
     1501 cttgatccca tcgtaaccga cgctgaacgg gtagtcacag cgactcggcg cttcggccgc
     1561 acactcggga cgcagccgct tcacgccgga gctatcgagg aagaatggca acgagcagcg
     1621 cgcggcgacc ccggattcgt agctctgcgc accgtcccgt cggcgtgagt cgagccgacg
     1681 cagccgctcc tccaacattc gcacacgctg ccgcgtgtct tccagttcgc gagcgatccg
     1741 cgctcgttct gcatcctcgt ccggatcggc ggccgcgatg cggctcgccc ctgtgatggc
     1801 gacggatagc gctatgagcg ctgcagcacg ctgaaatatc tgcctgggcc cgaaggacat
     1861 ggcgttggtt aatgtacctc actccgtgaa tcgagcactc gagtgccttc gattcggtgc
     1921 tctcttgtgc gtgttcgtcg caggcagcgg ctgactgcag cctgcgcctt cgcactctgg
     1981 ggacgtcgcg agtgcttcga gagggtagcc gaaacgttcc gggctggaac aaagctttgc
     2041 cttacgtgat aggtgccgcc tcgtcctcat ctttgcgaac tctgcgccgt tcttcgaggc
     2101 gcatggcatt ctggcacgtc tcacgtttgc ttctgacctc cttcccctcg gtgttggtgt
     2161 gatgcggagt ctcagcgttg gacaacgaat cctgggtggg ttcgcggcgg tgctcgtagt
     2221 cacggccctg ctgggcgcgt tcgtgttgct gcgcttacgc gtagtggatg aggagttcaa
     2281 gcgcagcgct cagcagagcg tgcccgccat cgttttggcc gggcaaattc gagcaatcat
     2341 caaagaggac cagatcggta ttctgcgcta cctgctcaca cctgcgacca acctggatga
     2401 gcatcgaatc atcgaggatg aagctcgaca acgctccgct gaagtgacgc ggggcttggc
     2461 ggagttgagg aaattgctga gcgaccccga acagctgcag atgcacgatg aagtgctgaa
     2521 ggcgcgcgaa aattatctat ccgtacgccg tgagttgttc gtgaatagcc gcaataacgc
     2581 ggtagagcgc gagtcgctct tgccacgcgc gttgccggct tatcaggcct acattgcgca
     2641 gctgaaccgg atcgtggagc agtgttcgaa aggctcggcg cgctctcttt cggccgtgag
     2701 tgacgcgacc gaatcgaccc gctacggggt gtggatcggg ctgggtatcg cgctgctctg
     2761 cggcggagcc attgcttttc aaatcgtgct cagcaccacc ggagccatcc gcgcggtggc
     2821 gggtcggcta gggcgcagct ctgcgcacgt ggcgtcagct gcctcacatc tgaaagcgtc
     2881 ctcgcacaca ttcgccggtg gcgcctccga acaagccgcc tcactcgaag aaaccagcgc
     2941 ttccatcgaa gagatttcgt cgaccatcgc gcaaaatgcc gactcggcgg tgcgggccaa
     3001 acagctctcg gacgaagcgc gctccgctgc ggagcagggg gcgaacgata tcgaatcgat
     3061 gactgccgcc atggaagcga tccgcatctc ttccaacaac gtgaccaaga tcatcaagac
     3121 gatcgacgag atcgcgtttc agaccaacat tctggccttg aacgcagccg tggaggccgc
     3181 tcgcgccggc gaggcaggag caggtttcgc cgtggtggcc gaggaggtac gcagcttggc
     3241 ccaacgctcc gcgagcgccg cccgtgaaac cgccgccaag atcgccgatg ccatcgacaa
     3301 gagcgagcag ggggcggaag tgtccacccg cgtctcggcg tcgcttggca gcatcgtgac
     3361 caaggtccgg aatgttaact ctttgatcgg ggaaattgcg tccgcgtccc aggaacaatc
     3421 caagggcatc agccaagttt cgacggcggt ggctcagatg gatcgcgtca cccaaaacaa
     3481 cgccgccggg gcgcaggagt ccgcgagcgc ggccgaagag ctcagctccc aagccgcact
     3541 actcgacgct gcggtcggag agctcgagga actgttcggc gaatcgcact ctcgttcact
     3601 cgggagcgac gcggagatcc gaacaatgcc gcagcgcggc tcacgagctc gtatgcggct
     3661 cgcgagctga atagcagccc gcaggcaggc ggagcaccac taacgtggcg ctccgcggat
     3721 tcgcacccgg cgactcagac cggacgatcc gaggcgttca gtgcgatcgg cagtacggcc
     3781 tctttgatgg ctgcgctcaa ggagccctcg attgccccgc catctcggcg ctgcagttcc
     3841 actcgctcga cgagcccggt gggccgtgtg cgtacgtgat aggacgccac cacgatgcca
     3901 agcgcgaaca aggttcggtg caacgcggaa accacgggga aacgagactc gcgaccaatt
     3961 tcgattgcgg tgagttgggt gccttgttgc tcgaagtgca cttgagcttg ggcctgcacg
     4021 gacggcgagc gcgtttgccc gcctgcctga ttgatcgatt gcatcgaact gtcttgcgtc
     4081 gaagcgtctg gcttcgaagt ttcgttggct tcgcggatcc cctccaaatc ggagatttcg
     4141 cgcacctgga gcccgttcgg agtgcggtcg tgcgcaacac ttcggcgcgg cttcgcgtaa
     4201 cctccaaacc gcactcgctc agagcgtgct cgttgcggct tttcgctcaa ggcctcagcg
     4261 gcgggaccga gcaagccggc gtccaacgtg gacggcaaca cggcgtgcaa gtcggtcgtg
     4321 gaaagttctg cgataggagc ggctgcgttg tgcacttgcg atctcgaatc cggctctgca
     4381 gtaatgattg aacgtgatga ctggagtgtg atgattgaag gcagtcgaac gggttgaggc
     4441 acggaagtgg gccggccaac gggcgttcgg cgccctgttg gtcccgggcg tttgagcgcc
     4501 ctgttggtcc cgagcgtttg aaagccctgt tgatcccacg cgtgcggccc cgtttggatg
     4561 ccgcccaatt cactggccta aaatcattcg ccgcctttgc tcgaatgatg ccaagttctc
     4621 tgacgcggtt tatctgctca cggaaccgag acctgtcaat cgcggagggg tgacatttcc
     4681 gtgcctttgc ccgtggtttt tgcgcttgcg tgtcgacgca gtggcggcac cctccgggcg
     4741 tgagtagacc ccacggacac atgaccgcct cagcgtcgca aactcaccgc accattcgcg
     4801 ccatctacgg aatagaagcg ccgaagctgg tggcgcgcct tgcgcgcttg ttgcgcgacg
     4861 tgggtttggc tgaagagttg gctcaagacg cgctggtcgc cgcgctggaa aactggcccg
     4921 gctcgggtat tcccgaaaat cctggggcct ggctcatggc gacggcccag cgccgggcca
     4981 tcgacgaact gcggcgcggg cgtcgtgtgg agcaaaagca cgatctgttg gagcaagagc
     5041 tcgcgctcgc gcaaagcttg gccgaaccgg acggcgccgc ggacgcgagc gtcggcgacg
     5101 acctcttgcg gttgatgttg atctcgtgtc acccggtgct gccgccggag ggccgcgtcg
     5161 cgctcactct ccggttgctg ggcggcttga ccacggagga aattgcccgc gccttcttgg
     5221 ttccggagcc gacggtcgcc cagcgtatcg ttcgtgcgaa acgcacgctg gcagagcggc
     5281 gtgtgccctt cgacttgccc cgcggtgcag agctcacgga acgggtggga tccgtgctcg
     5341 aggtgctcta tctcattttc aacgaaggtt actccgccac ctccggggac acctggttgc
     5401 gtccggactt gtgcgaggag gcactgcgtt tggggcgcgt tctcgcagag ctcgctccgc
     5461 aggagcccga agtccacggc tgcatcgcgc tgttggagct gcaagcctca cgcatggcgg
     5521 cgcgagtgga cgcgcagggg gcgccaatct tgctgctcga tcaggaccga agacgctggg
     5581 acatgctgat gatccgacgt ggactggcgg ccttggcgcg cgccgagcgg ctcgggggag
     5641 tggacggccc gtacgcgctc caagcggcga ttgccgcctg tcacgcccat gccacgcgcg
     5701 cggaagatac ggattggcca aagatagcgg cgctctacac gcggctcgcg gagcgcgtcc
     5761 cctcgcccgt ggtcgagctc aaccgagcag tcgcgtttgg aatggcgttt gggcctcaag
     5821 caggcctaca cctggtcgac gagctgagaa agcatcccgc gctcgagcgc taccacctgc
     5881 tcccgagcgt gcgaggcgac ttactcatga agctcgctcg ttaccgcgag gcccgggagg
     5941 agctcgaacg tgccgcgagt ttgactcaga acgtgcgcga gcgcgcgtta ctcctgagcc
     6001 gcgcccgcga atgcgacaaa ctcaatgcca agtgagtttg gccgaaagtg tcagacctag
     6061 agattcggcc gacaaatggt cactacccaa gtgggcgtac tgcggtcgga tggctcccga
     6121 caaactgtag ccgcgactca gccaaattcc gataccgacc tcaggcgtga cggcgatccc
     6181 ggtgagttcg tggccgtagg gcaaccctcc accaccaaag ccctcggagc ggcgctgcac
     6241 cgacatttgg atcaaaccta gcgcaccgcc gaaatacagc ccttccgtgg gttcggggta
     6301 atagcgaacg aaggccgcga attgctgtac tcgcagcgcg agatccggca tctggaactc
     6361 gtcgttgcgc gtggagatcc agagcgggct cgccgagctg cgctcgtact cgcctgctag
     6421 taccagaccg ggcgcaacgg tacctccaat tgcgagcgtc aatgaaggaa agatcggcgc
     6481 ccacgtggtg gatcgcaggc gttgctggaa cccgttgctg atgctggcat tcgaagccaa
     6541 gcgcaatact cccacgttga gcccgccctc gatgaagagg ccatcgtgac gtcgatcgct
     6601 ggggcggctt tggggtggtg cggcccaggc cggcgtggcc cacaacacac acacgatcgt
     6661 actgcctagc tcgagcaatc tccgcgcgag caatcctggc tcgagcaatg gcagtcccgt
     6721 gatcggctta cacgatgcaa ggcgagcctt gggggcgagc tcgtttggcg gcgctttgcc
     6781 cgccgcgctg cggccgaagt accacgccaa agcgagagcc ggcagagcca ataaatcgct
     6841 gggatcggcc gtcgctcgta ccggttgcag aggagggagc gggctccgct cgagaaccgc
     6901 ggtgatcgcg aaccagggcc attgaacggc gcccaaacca tagcggtagg taaactccgc
     6961 aggagaccat agctcgaccg cgctatacgc gatcgccgtt actccaacgg agatacccaa
     7021 gattcggttc gaggtggcgc gaaaccgggt gggatttagg cctcggccag gccagagctc
     7081 ccacgcggtc tgtagccaga gcggcatcaa taccaaccca catacatctg agaatttgcc
     7141 ggtaaaaacg ccgggcatgg ccgctttgag gtagtggtca ttcacgatca aaccgaccaa
     7201 gctcagcaag gcgatcggat gaattagacc gccgcccggg gtcgaagcgc ttcgcctcat
     7261 gggccctcca cttcgctgat cagcaaatca ccttcgaagg gttctgggca ttggttgagt
     7321 tgcgccgtgg cggctccgtg ccaatcgagc accacctccg ggaatggaag cccgtccgcg
     7381 cgttgcacga tgaactcgac gcgagctttg cattcgcgcg ccccaaatgg acaacagccc
     7441 gggttccaag tgttggcgca cgacccgaat agtggcgtag accagctcac gctcgccgtc
     7501 ccgctgctgg gtggcggcaa agagctggag ggatcgcccg tggcgtcggc catcacacgg
     7561 ccccagatca gcatgcgcgg cagctcgcgc atgacggtag ggtcgggcgc gtccacatag
     7621 tggaggcttc ccgccatcga aaacacagcg gagctgacgg ccccgtctac cggccagagc
     7681 tctggtaggc ttgccaattc gatgtcgaat acggtgcgca cggtttctcc ggcgcgcgtc
     7741 aaagccatcg tccgttcact cgtcttctcc gtcagaaccg aggattcgcc ttcgaagcac
     7801 gcctgccgac cagaacagcc gacaaggaac aagctcgaaa gagcgatgat gagtcgaatg
     7861 caccgccaca cctctccaaa gctcgttttc acgattcagt cacctggacg gagataggaa
     7921 gttcgattgt ttcgctcgca gaattgttgc tagcgactcg ttcggatagt cgaatctcga
     7981 gctgcccgta cactgtaccc tcggccgcgc cgctctcctc ccgcgcgaac tcgatacgaa
     8041 agcgtgtacg gcacggggac gtggcagacg gcggatcgtt gcactgacct gcgaagggcg
     8101 cctcgaagga gtgttcgaag cgggtgagca ccgcctcctg ggtcggtttc ggatctggag
     8161 gctccaagcg cgcgatactc gctagtaccc agcgtgcttc cgcttctgcg ccgaacaccg
     8221 cttgcacgcg gacggagata ctccgcggcc actgcccgtc gggaaaggcg gccaactgat
     8281 ttacctcgat ctcgaaggca gtcgtcgggt gagatgcgtc gagttcgatg gggatgcttt
     8341 cgagccgccg ttcgcccgag taatacgagg acggttccgg cggcgaagta gccaacagcg
     8401 ccagcgaact ggccgagaac gcgagcccga ttaaccagcg ttggaccaga gttttggcgt
     8461 ctgccctcga gtgtttctca aaagccattt tgtgatccgg agttagagag atcttccgat
     8521 ctcggaacaa ggtcgcacgc actctctcag tactcaacgg cgcttcctcc tacccgtttg
     8581 cccatgacga gctctcgctg caagacgttt gccatcctga ttcgctgcag cgaaaggcgc
     8641 tccagaaccc gaatctccgg ctcgacgctg gctcgaattt accgctcgac caagaaccga
     8701 gtacgcgccg agagcccttg attgcaaacg aagcgggttt atcgcgaccc gtcggtggcg
     8761 ccgcgaatca cccacgcaga gccgaagagg gccgtccgag ccgcgcggtt ccaccttacg
     8821 gattaacgct cactgccgct cagccctgac gtgggagccc cgcgtcgttg caatttttgc
     8881 gcagcatgcc gaactccggc tggtgcgcat gtcggggttc gggccagagc aggaccaccc
     8941 cgcgaccgag ccaggaaaac gggcgcgagt cagagcagaa aaccagcgca gccccaatga
     9001 cgaatcaaat tggcaatctc tcgcggacac atgaccgcga ttgacaagcc ggcgaagctc
     9061 ccccaggttc tgcgcgtgcc agagagagct cgcaaagtta agccgttctt gcgacgattt
     9121 gggcggtttc tgctaggcct ggtcgcggcg tcgattttgc tcaccctcgt gctgctcgtc
     9181 gacggctggc gcgcgttcgg tcaccgcgcg gagggtcaac ggttggagcg catgcagcgc
     9241 tcacctcagt ttcgggatgg gcacttcgtg aacccgcagc cactcgtcaa tcacctcctg
     9301 ctcatgttcg aagggctcct gcatgtgagc cccaacgcca gcccatccac gcaaatccca
     9361 accgtagcgg tggaccccaa aacattcgtg gtcccgcccg tgagcggact gcgggtaact
     9421 tggctcggtc acgcgacgac cttgatcgag atcgatggca aacacgtgct caccgatccg
     9481 gtgtggagcg agcgggcatc cccgttcacg tggatcggac cgcggcgctg gtacactcca
     9541 cgtatttcgt tggcagagtt accggttttg gatgcggtcg tgatctctca cgaccactac
     9601 gatcacctcg attaccgaac gatgctagcc ctgaacgagc tgatctcgaa ccgggggtgg
     9661 gccacgcgct tcgtcgttcc gctcggaatc ggcgctcact tggcgtactg ggggattccc
     9721 gagtcccgca tcgtagagtt ggactggtgg gagaagacta gtctagggga ggcggctgac
     9781 gacaagctgg aaatcgtgtg tactcctgcg cgccacgctt cgggccgcat gctgctcgac
     9841 aacgacgcta agctatgggc gagctacgcg ctgattgggc ccgcccaccg agcttacttc
     9901 tctggcgata ccgggctgtt tccggcgctg cgtgacattg ggcagcggct aggcccgttc
     9961 gatctcacct tgattgaagt aggtcagtac cacggcggtt ggcccgattg gcacatcggc
    10021 ccggagcagg cgctcacggc acaccagtgg ctgcgtgggc gagtcttcgt gcccattcat
    10081 tgggggcttt tcgctttggc ctatcacggc tggacagagc ctgccgagcg cgtgatcgcc
    10141 gcggcacgta ccgctcaatc cgtcgtgatc ttgcccatgc ccggggagtc cgtcgagccg
    10201 tcggtgcccg aggcggaggc tcgagccagc cggccccgtt ggtggccgaa gctgccgtgg
    10261 cagagtgccg ccgaagcacc aatcgtctcg actcagattc agtgagcgct attgaactcg
    10321 agtgctgggc ttcattcaag tgctccgcca aagcgcgcgg agatatgcat ataggttcct
    10381 caatcgtgcg acatgcggtg ttgcatcgtc gagggggtgc cgcgcagacg ccaacccgaa
    10441 gccagccctt gcagggcacc ccttccgaac agggtggcgc gccgaagccc cgtcggacgg
    10501 cggccgctaa tcaccgccgc ggccgcttcg gcgttcagtt ttacgaccag cttggtcagc
    10561 atcagagccg ctctatattg gccgctgcgc aggcacttgg cggtgaccgc gccgagtcca
    10621 atgccgtcgc ggctccagag tactcgactt tccgggccat gcgcgcgcac accgtcgtgc
    10681 cagacgctgg cggttggctc gactaccacg cgcattccgc gcgcggcggc gcgcagcgac
    10741 aaatcgatat cttcgccgct cggcaggggc gagcctggtc cgatctgctc atcgaatgcg
    10801 ccgagtgctt cgagtacgcg ccgtttggcg ctcatgtttg cgcccatgcc cagcaccaag
    10861 tcaccctcga cgcgcccttg cgctacgatc ttttgcggtt cgaacgcggg gacctcgccg
    10921 tcggcgccag tgaatcctgg tggaggcaac acggcgccga acaccatgtc cgtcgacggc
    10981 tcgtcgcgat aacgcgcgac gtgacgcgcg acccaatctg ggctggcgat gcagtcgtca
    11041 tcggtgaaca agattatctc ggaggtatcg ttggccatgc cgatattgcg cgctcgcgag
    11101 accccccgac cctcgtccag caccaagcgt agccgtgcat cggacagctg cgctccgagc
    11161 tctcgctggg cccacgacgg gcctccaccc tcggcgttgc cttgcgcaac caccaccaac
    11221 gacagtcgcg gatagtcatt gcgtagaacg ctctccacgg cggctttcaa gcggccgggt
    11281 cggttgcttc caatcaccac gcaaacggag ggcagggagg cggcttcctg gctcattcat
    11341 tcctcgtctg ccctcgaata gcatgtcggc gccacctgaa cacgctcggt cgagccgtac
    11401 cgacacgctc gagaggaacg tggctcgaag ttggtgtctg ggtgagaagc cccgactgtc
    11461 cgtgaagccc tcgagcgcag cgttgggttt cgaaccggtg acgcttggaa gctccggccc
    11521 cgatgtcacg cgaacttcgc aaactaaggc gccggtagca aagcccgagc tctcttgaac
    11581 aaggtctcga attccatgcg gtcggccgcc gttccgcggg cgctctggat caaggccgcg
    11641 atctcctcga gatgagccgg ttctgcgtac gggctctgct tcaggatttc ggcgaagctc
    11701 gcgaccgcaa ccgcccactt gaagtccgca tccagtgcct cggagctgtc cgcgacgtcg
    11761 gacgcactca agctcgagct cacctcagcc gccgcgtcgg tctcgagggc gcctggctgc
    11821 ttgtaacgaa tcttgacgag cacgatgtct tcgggagcgc attcgatggc tccgtcgtaa
    11881 acggcaccat cctcgagcgc gggagcgccg ctgggcgcgg gtacggcggc gcccttgggc
    11941 actacttcgt agagcgcagt cacgcggtga ccggcaccaa cttctcccgc gtcgacctga
    12001 tcgtcacgaa agtccgtgtc ggcgatggct cggtcttcat acccgagcag ccgataggcg
    12061 agcacgcgat tcgcgttgaa ctcgacctga attttcatgt ccttcgcgat gtggatcatg
    12121 gtcgagagca gccggccgtg ggcgtattgt atggcttgat cggcgttgga gattacggag
    12181 tacatgccat tgccggcgtt gctcactttc tccatcatgg agtcgttcag gttgccggaa
    12241 ccaaaaccca gcgtcgtgag tgttacgccg gacttgcgct tctcgacgac gagattcagc
    12301 agatccgtgt cggaggagag ccccacgttg aagtccccgt ccgtgcacat caccacgtga
    12361 ttgatgccgc cttcgatgaa cccggctcga gcctgatcgt acgcgagctg catgcccgct
    12421 ccaccggccg tgctcccgcc cgcttccagg ccctgaatga cggcggtaat ctccgcttta
    12481 ttcgcgacgg gcgtcggcgg cagccgcagc agcgtcgctc ctgcatacgt gacaatcgag
    12541 atcttgtccg tcggcttgag tacgtccagt gcttccgtga gcacggtctt gaccagcggc
    12601 agcttgtctt gttctgccat gcttccggat acgtcgatca aaaacacgag attggcggta
    12661 ccgggatctg cgggggcgag cttgccttga attccgaccc gcaatagtag cgtctcggct
    12721 cgctgcacgt tgggcgcggc cgccaggtgg atcgagaacg gcacctcgga ttcggactcc
    12781 ggcgcgggat actggtaggc gaagctgttt acgtactctt ccaggcgtac gctttcgggt
    12841 tggggtaaca cggacgtttg cacgtctcga cgaaagatat cgtagctggc ggtgtcggcg
    12901 tctgcggcaa aggtcgagag cgggtcatac tcggctagca cgaacgggtt ggtccccacg
    12961 gcctcgtact tgtcgccgga gttgggggga ggggcgggct cgtccgcgcc cgcactcggg
    13021 ccgaaagtga aggagccacc cgaggcgctt aggacgggtg aagacgtgtt tccgccctgc
    13081 gccgtcgagg gttgcggctg cgctccgttg gcgggcggct gcgccgctcc gttggcgggc
    13141 ggctgcgccg ctccgttatt ggacccggcg tagctcgatg agccagggcc ttggctcgga
    13201 acgggagtcc ggggtcgcgc agtggcccca tcatcggtac tagacgcaca gccccacgct
    13261 gccggcaaga gcaacaacag catcgagccg atcccaaccc acttgcgagt cctcttcatg
    13321 gcaagcacct cggtgcggcc ggcgaagcaa gccgcgcgcc aacgcggaaa cgggcagcgc
    13381 aacgaacaca cgctcgcttt gcgtgcgata tcggggtcaa gctgtgccga cttgtgccgc
    13441 gctctcaggt cttgggaatg acgagggtcg cgaagctctg cgcgggcagc tggaccaagt
    13501 gtcgcctttg gccggcctga acttggaggt caattgggct gttgccctcg ttcaggagct
    13561 cgacgatcaa ggttccgtct ggattcttga acgcgatctg atcggcatag ctgctcaggg
    13621 tcgcgatgcg ttgtgctcct gggcgcacca agctggagaa gtgcttggtc atccaataca
    13681 gcggcgtgta agtcacttgc cgagtttgac ggtccactac gatcgcgctg ttctgtggcc
    13741 agggtagctg cgaatccagg ttcttgccgt gctcgtcgag caccatgttc caaagcatgt
    13801 agctcgaggc gccggcctgg atgtagtctc ggaatttgcg aaaggtgtag gcggcgtacg
    13861 tgaagtcgtt ttgcggtgtc tcgggctgaa attccggctc ccaaggatga ttgccgcaat
    13921 ccgtctcgga ctgcatgaac gtgagctgcg ggtaagccga gaggatcggg cccgcgtgcg
    13981 acaggccaaa ccactgcaag gcaatgcccg agacgtagct tcgggcttcg gggtcgttca
    14041 gtacgctgag ggcgtcccat tcggggcggt tcacggtgcc gacgtaaatg tccgtccgca
    14101 acctgcgttt tcgaaaggtc gggcccaagt ggtcgcgaat gaacgtgatg tattggttcg
    14161 gcgtccagtc gcaacttggg tagcgcgtga gctgccccgg ttcattctgg ggcaccagca
    14221 tcgaaatgtt caggccttcc cgcgcgtact cctcgacgaa gcgggccaga tagagagcat
    14281 atgcggcgta cacgagtgtg tcgtccttca tggctccgct gtcgaaggac tggttcgtct
    14341 tcatccacgt cggaggcgtc cacgccgagc cccagagctt gagatcgggt cgaatggatc
    14401 gcgcggcttg gatgtaaggg atgagccgct cgcgatcgcg cgcgatggag aactggtcca
    14461 tcgcgaaatc tccgggagtt tcgtccaggg tatagcggtc gatcgcgtag tcgctcgcgc
    14521 cgattggtac gcgacagtag ttgaatccca ggccctgttc cggatcgaac agcgctcgga
    14581 gtacttgcgc acgctccgcc gcgtcgagca ctccgagcgc atcccagcca tgctcgttga
    14641 acgcaccacc gaacccttgc atggtctgaa aggcctgagc tggatcgatt cggacctcta
    14701 cggcggtgct cggggcgggt tcgctcgtac cggcagatgt caccgctcgt tttgccaggt
    14761 gttcgtcacc ggtcgtaacg agccattcga ctgaactctt cccaaccacc ccgggtactg
    14821 cggccgcgcg cgggggcgct tctgccggaa cggaaccaga cggcgaacac gccgccgacg
    14881 agagcccaag agcgatgaga ccgaacgcgc gccacgacat gggtcggcag tctatttcga
    14941 agccgctttt cggcacaact gccgtgcgct ctggccggcg tgcgcgcggg aaatcactca
    15001 gtaagccgcg agccccgccg cggtagcggg cagtgagagc tcgctctcgc tcaggttcaa
    15061 gttgccgtcg ggagcgatac gaaagtgact gatgccatgg ttgccggcgt ctaggaggta
    15121 caaatctcga tctacgacgg ccatatctag cggtcggctg ttctgtcccg cggcacccgt
    15181 tattccgtcg ggatggagca gcgttagacc accgttcgga tcgacgtgat agcccgagat
    15241 cgagctgcta ccggcattag cgatataggc gaaatgaccg tcgggactga cggttgccca
    15301 gcagggggcg cgctgcaaat cgggaaccgc cgcgctgatc accgtcacct ggcccgagcg
    15361 gtgaacgtcg tacgatgagg tggacgccgt tcccgcctcc gatacgatca agtcaccttg
    15421 gggagtaaag gtgaagccga acggcgtagt tccagccgac gcaaaagctc cgcgtggggt
    15481 gagcgcgccg ctgcgtccga tgccgaaggt ggagatgcga ctggtggcct tctcggtgac
    15541 gacgagcacg tttccgcgcg gcgagattcc gacttgggct gggcccgcgg cggtcgcgct
    15601 gagcggctga atcgagccgg gtatgggcgt cagggtgcca tcgggcccga gccggtagcc
    15661 cgccacgttt gcgggttcac cggcattgac cacgtagacc cggtggtcct gaatggcgac
    15721 cgaaacggga cgggtgccgt tggagctcac gtggctgcgc aaagacagct cggcgccgtg
    15781 tactgcaaag ctcgtgatct cgttgctacc ggcgtcgacg gtcaccagaa atcggccttc
    15841 ttcactcacg gcgagcgcgc cttgggagcc tagccccatt cccgtgccca atcctccggt
    15901 tgcgtagtct ccgaccatct ccagattgcc tcgtggggac ctacggaacg cgagcacact
    15961 attgccttcg gcgctgttgc tcgaggtgaa gaccgcgctt gggcgtccat ggccgcgcag
    16021 tgcttcgctc gctgagctcg cttccgcgtc gtcggactcg gaaaccgcgg ccgaacacgc
    16081 gaacgtaccg gccaaacaca cgacaccaac cgccaaatga ggacgaaaca tgctcacgcc
    16141 tttcgtggga gaacgctgag tcggccatga ttgccgactg cgtgccgttc cctacgagca
    16201 gacgagcgag gggttacaac gctccgcaga ttgagcaacg ggctgctcgt tggcccgcgt
    16261 gcctcgcggc tgcctgcctg accgccagcc tgcctgaccg ccagcgcctt ttggcgaacc
    16321 tacttgccgc cgagactgaa gcccgccgaa gctttggcac ctgcagacgc tttagccttg
    16381 gttcctgcgc tcgcccccag gttgacgttg agcttgggcg gctgaatttc gattctcggc
    16441 gcagtcactt tgacggcagc gccggccttg gccgcggctc cgcttgcagc attgagcttg
    16501 ctctttacgt cgagcgtcgg cgctttggca tccacctgca cgcccgcgcc tagattggcc
    16561 ttcgcgccaa gcgccgcatc cccggcgagc ttggcggaag cagaggcccg catggcgccg
    16621 tcgagagagg cgagcgcgcc atagaaaccg ccgaggttca cgtgtagcgg caccactcga
    16681 aggttcaact tgggtgcttg gaccgacacg taacatcggc aactcgcgtc ccaatcgaag
    16741 tccagctggg tcgcggcccg agcacccgcg aaagctcgta gcttgaggtt ggcgtcgagc
    16801 atcgggctgc ccaacttgtc gaacacgtac gcccgcagct tagggccttc ggcgacgagc
    16861 tccactccgt agctgccgag cgcgagcatc gctccgccat gcgaggcacg cgccgaaatt
    16921 ccggcgtcga gcagagctcc cacactctcg agtccaccga ccttgacgtg cagagccaac
    16981 gggccaggaa cgaagcgagc ggagccctcg agcttgcccg aaaaacactg gctctccgcg
    17041 tcgaacgtga ggccgagacg agccgccttg tcgagcgagg ttgtcacgtt cgcgctgacg
    17101 tccgctcccg ctttcagcaa cgcgcccgtt gcgtcccgca accgggcgcg gatctctcca
    17161 cctgcgtcca gtaccacctc cgccgagtac tgccccaccg ccagcatgtg accgccgagg
    17221 gtggcttccg ggagcgccgc cgccagggcg agcttgcctt gcaacgcctg ctcacccact
    17281 ttgagctcca cgccgatagg gccggaggtg agctcgacac cagccgccgc ctgccccacg
    17341 aagcgcgcct gcggagccgc gaagtccagg ttcacccgtt cgcttttccc gcccgatgcc
    17401 tgcacagtga cggcaagctc tgcggctgca ttcaacggtt tgcccgaagc gtccaagact
    17461 aaagcttcga tgcggccgct ctgatacggc gcaacctcga cttggaacgt gccgactcgg
    17521 accaaactgc cgccgatccg tggagtcgtg agctcggcca gagcggcggc cgacacggtt
    17581 gccgaggccg aagcgttgcc gctcagcgac gtgccattcg gaggccgtgg ggacgcttcc
    17641 gcggatgctt ccgcacctgg ctccgtcttc gcgctctcgc tcttggagca tgcggcgccc
    17701 cacatcagtg gcagcgcgac cgcccagaac gctggtttgg acaagccgga gagaaagaga
    17761 gcggagccgc ttcgccgaat ggtgcgggtc attgggctga gcactatcca acacggacgg
    17821 gctgatcgca aaccgctcgg cttgctggcc tgagcccggt gaacgtgacg cgcgaaaccg
    17881 gcccgagtgc gagctctact tacctcttct gttcggctgc tcgtcgggct ttttgaggga
    17941 ttccaagcga ttgacatgtc tcgccgaggg gaccactcat cccacgtggc agctctcaac
    18001 agacgcagca cggctcgaga cgctgtgcgt ggtgtatcgt tggccggtaa gcacgcgatc
    18061 gtgacgggcg ccagctccgg cctcggcgtg gagacggcac gcgctttgtc gcaggcagga
    18121 gcgaacgtcg tactcgcggt gcgtgacgtg gcggctggtg agcgtttggc aggcacgcta
    18181 gttggtgcgg gcgtgggacg cgtgacggtc agcccgctcg acctctctga tctcgcttcg
    18241 gtgaacgcgt tcgtggagcg gcaccgaggg gggccgctag accttctcgt caacaacgct
    18301 gggatcatgg cgacgccgtt gggctatacg cgccaaggcc tcgaactgca gttgggcacc
    18361 aatcacgtcg gccatgtggc gctcacgctc gggctgctgc ctacgctcga gcagtcgagc
    18421 ggggcacgcg tcgtgaccgt ctcgagcgcg ctccacagcc gcggtcgggg tggccgtttg
    18481 ttggagactc tggagcagga tcttcgctac gagcggcgca agtacgtacc cttcgacgcg
    18541 tatggtgatt cgaagctcgc gaatgtactg ttcacgctcg agctagcccg gcgtctaccg
    18601 ccgcaagtgc gggcgttctc tctacacccc ggagtgattc cgacgccttt gtcgcgcagc
    18661 cttggcgtgg gcggcgtctt gttccgcacg ttgggggccc cattcatgaa gagcgtggct
    18721 caaggggcgg ctacgtcggt cttcgcggcc accgccccag agctcgatct acattcgggt
    18781 gcttacctgt ccgactgcgc aatagcgatg ccctctcgtg aggcgactga ccccgagctc
    18841 gccgagcgtg tatggcgagc gactgaggct tttatcaaag cgcgctgatg tcaggggttc
    18901 gtgcaacccg gtaacggcgg ccaggttggc gaaaacgggt tggcgaaccg ggcgggcgaa
    18961 ccgggtggcg gaaacgtggc gccgcctcga gccgcgctgc cacagcagga taatcgcgtg
    19021 attccccagc gtaaccagct ccggggcggc gggtccgccc ggcgagcgtc tgcaagattc
    19081 tgattgcccg cgctcctctg gcgtcgttca ctccgaagcg catgagtgct ttcacgttcc
    19141 acgtcagccc gttatcggaa tcacaggtct atcgcacgat cgaggccgcc ggctctgcaa
    19201 ccctcgaaca gctgcatcga gcgatcgttg ctgctttcaa tctcgaccaa gagccggcgc
    19261 acgccttctt tttgaacggg cacgcttggg acgccgagtt cggcgtttgg ggttgtgcgg
    19321 accagagtca acgtgcctcg agcgagacgc gcatcgagag cctcgagttg gtggagcgca
    19381 agcgcatcct ctacctctac gacttcgacg cggagatctg cctggatgtg cgggtcacgc
    19441 gcatcgaaaa ggatgcgtcg ggcccagccc aacctgcgct cgtgggctcc gcgggcgagc
    19501 ttccgccttc ttatctggag ccttcctcgg acgctgacga agacgaggaa gaagacgaag
    19561 agcaggccga tgccgaggcc gaggcgaagc gtgccgagct gctggccgct gcggagccca
    19621 ctttggctgc attggccgag cgggtgagag cctgcatcga gttcgccgag gtgcgcttgc
    19681 agatgcagtt cgacgagatg gaagagcact tcgaggatgc cgccgtctcc ggagccggtg
    19741 aggagggctc gccctccggg cagcacgaag cggttgaaga gggcgaaggc ggcgaaccgg
    19801 gggaagccga cgagcacgat gacgaggaag aagggcccag cgccgaggag gaagccgagc
    19861 tcgccgaaga gttgctgact cgcttcggca cggatgcgca gcggattacg gagcagctgg
    19921 agccgctggt cgacgccgac gtgttcgatt ggctcgagag cgtgccgcaa cgactcagtg
    19981 acgaggggga cgccaagcgc gccttcgctc tcgcgcagcg catcgttcaa ctgccctggt
    20041 cggagagctt gcgggcgctt ctaccgcggc ttgccgtccg agccgatcgt cccgacgaag
    20101 ccttgagctt gatcgaggtg ctcttgcggg atgaacctca ggacccggag atactgctgc
    20161 agattgcgct cgccttcgac gagctcgatc aggggacgag cgccgagcag tactaccgtg
    20221 aagccttgcg ctacgccggc tcggacatgg atctgcggag cgagattatc gagcacctcg
    20281 tcgagctcct gcgtcagctc ggtcgagacg atcaggcgaa caaggtgatg caagcggagg
    20341 tgttgcatac ccagcgcttc tcgccactat tcgggccttc gagtccgcct tcggagccgt
    20401 tccgaaacct gctacccaag gtcgggcgca acgagccgtg cccgtgcgga agcggcaaga
    20461 agttcaagaa gtgctgtggc ctcaactgac ggtcccgagc cgagctgtgt gacgacccgc
    20521 ctgggctgac tgtgctgagc ggaggtgctc gcgaggcctc atgatcgacc gccacgggcg
    20581 cggatcatga ggccttggcc actttgcgag ctgccgctct cgagcacgac cttcgctcac
    20641 gaggcggtca tcgacgacgc aagcgcgtgc agcgggcgca gcttgtccgg gtttcggacg
    20701 atattgatct cgcagatccg ttcgccatcg gtctcgaacg tgatggccgt gacggggatg
    20761 ccaccatgaa gcagcaccaa gcctggccca ccgttgatcg gcaatagatg gtaacgcgct
    20821 gcgctttcgg ccgtcttacg agccagcccg agcgcgaagc gtgccacgcg atcgaccccg
    20881 atgatgagcc ggcgcgccgc ttgagccttg ccgccgtgat ccgtgcgggc gaccacgtcc
    20941 gtggccagca cccggcccag ccgttcggga tctccggtgc tacacgcgat caggaactct
    21001 tgcaccaagc gagcatgcac ggccggcgca tatcgaaagc gcggcttgcg cgactcgata
    21061 tgctcgctgg ctcggtgcaa gagttgacgg gacgtggtga catccacgcc gagaaattcg
    21121 gagatctccg cgtgattgta gtcgaacacc ttggccaaca cgaagacggc gcgttcccgg
    21181 gggctgagtg tctcgagcag cagtagaaag gcggtggaaa tcgagcctgg gtcccgctcc
    21241 atgcccgtga ccacgggttc gggcagccac gggccctcgt aacgctcgcg tcgccagcgc
    21301 gcgctcgcaa gcacgttgat cgccattcgg gtgaccatgg tcgtgagcaa cgcgctcgga
    21361 ttacggatct cggccgcgtc caggtcacga taacgtatgt atgcgtcctg cacgacgtct
    21421 tccgcttcgg ccgcgctacc gagcaatcga tacgcgagcg cgaccagcct tggccgctct
    21481 tcttcgaaca cgccgtcgct catgtcgcgt atgccatggt cgacttgctg gaaggctgca
    21541 agcctcgacg atgggcggcc tgcttcgctt ctcgctccag cgtccaaaag gtacctcggc
    21601 agatcccttc tttgagccag gctgcggcgt gcccagccac gctgaattct cgaagctcgc
    21661 cgttgggctc gtgcagctga atcaagcccg cgtcgcgacc caagctcatg cagcgcaaca
    21721 tgtcttgtac acgccacggt tcgggagcgt gaccctcccg ctcgcgggca atgttttcag
    21781 ccgcgtggag gccggcgggc attccgagcc gacacgacat gagcgcgggc gcccccacgt
    21841 cgacgttcgg ctgggccgcg tccccgacgc catagatctc gggataactt ggcacgcgca
    21901 gcgttgcgtc aaccaccaag cggttggcgc cacaagtggg aagaccgctc tctttcgcca
    21961 aacccggcgc cgcgaatccg gtcgcccaca cgcaaacatc gaacgggagc gtgagctggg
    22021 gcccgatcac gcgacccgcc gcgacttcgc tgacctcgac gcattcgtgc aagtgtaccc
    22081 cgagctcgag caatcgggca cgaaccttgg ctgctccgga acgagacaaa gtgccggtgc
    22141 ctagcgcctc gcgcgctacg agtcgcacct cgagctcggg ccgcatttcg gccaattctg
    22201 cggcaaactc gagaccggtg aggccaccgc ccacgatcac cactcgggct cctcggctga
    22261 gcgccaccaa ctccgaagcg agactccgag cactgctctc ggacgcgatc gaggccgcgt
    22321 gcaggcgcgc tccgggtaca ccagggtcct ggtccgagcc gagcgcatag accaagtggc
    22381 gataggagag tgtgccctcg tgcgtcagca ccgtgcgaga cttggcgtcg atcccgagga
    22441 cgcgagtatg gatgaaggaa atgccactct gcgcgagcca ggggccgagc ttcggcgagc
    22501 gcgcctcgta ccccaaggcc acttggtgca gacgaatgcg ctcacagaag ctatcccgcg
    22561 cgtccactac cgtcacgggt agcgtgggct ttctggccgc cagtcggaac gcgcagctca
    22621 acccggcgta accagcgccc aacaccaaaa cattgtcgtc tcgactcata cgaacgtcac
    22681 ctccgcctga ctgacaagac cccgggggag ggcgtgacag agtcgaacaa aaaaacgtcc
    22741 ccggctgggt tcgtgcgtgt cgcgctgagc gtttggggtg ctgcggggtg cgtcgagcgg
    22801 ctcttctcgg ctgctggaag gcacagagtg ggagggttgc acgcacaaaa cggtttgcgc
    22861 aagcggtcac ttcgtcggtc gggccgagcc cccgtcactc aagtgtatcc ccgcaatgaa
    22921 tcgagaggcg agcagagctt gggcccattc atccatttct ctgagccgct tgctcgggac
    22981 gagcacggcg ctgatcgcga cactcgcgac gggttgccaa gtcgagacaa ggagttggcc
    23041 gaccgagcgt cgtgattcgt cagacattct ggttcgggat ttgaggctca attcggacca
    23101 ggaactcgaa gtcttgcgcg gagtctcagt cttgaagggc gggctcagtc tcggcgggtc
    23161 cgtcacggat ctatccgcgc tcgagcagct gacgcacata gccggcgacc tgcgcgtgga
    23221 cgcggctcgg ctagagagcc tctcgggcct cgagcgactg cactccgtcg atggctcgct
    23281 caacattgcc ggcgcggagc tggtgaactt ggaaggcatc ggagggctgt cgcaggtggg
    23341 cggctatttc tacgtcagcg gcgagcacct gcgcagcctg aagggcctgt tcgggttgcg
    23401 gcgagttcgc ggagacctct tgctgagcgg taagtgcccg gcgctccgtg atttcagcgg
    23461 tctcgagcgt ttggtcgaag tcgatggtga cgtatacata gagaaccacc acgtcgtgag
    23521 cctgcgcgga ttggacggcc tgcgtgatgt gcggggcggt ctctacgtat atgctgacct
    23581 gaccgacttg acgggcctcg gtgcgttgga atcggtgggc ggaaacctgg tcttggagag
    23641 cctggcgctg agtggtctcc acggtttgat gggtgtccgt cagattggag gctacttgag
    23701 ctttcgctac accggcgtga ccgatctctc ggttctcgaa cgcctcgatc ggctcactcg
    23761 agtcgaattt cacggctccc agtgaagcgg ggcgcggccg ggctgccgcg caagcgttat
    23821 gctccatggc atgccgcgtt ctcggaagac tcgtcccgcc gccaccgaag tgctgtgccc
    23881 attttgcgga gagcccgagg ccatccatat cgacaccggc ggcgggccgc atcaagtcta
    23941 cacggaagat tgtccggtgt gctgccgccc gcgcgtcgtg cacgtggagc ccggcgactc
    24001 tcccggcgag tacaacgtct ggctcgagcg ggaagagtag ggcagagcca agatccagta
    24061 tctagacaca gtagtagcta gggaaacgta gcctagaggc agaagccgag taggcaggtc
    24121 tcctttttgg cgcatttgtt gccgcagtcg ccacagttgt tgccatcgaa caagaggtcg
    24181 acgcaggcgc tgccacattg cgcgtagccc gcgtcgcagc gcacaccgca cgtgccctct
    24241 ttgcactccg ccttgccatt ctccggcgcg gagcaggcct tgttacaggc tccgcagttg
    24301 ttcaagtccc gtgaggtgtc cacacaagcg tccgcgcaag cgctaagccc cgcctggcag
    24361 gtgaactcgc agcgtttttc tcggcacacc gcggtgccgt tttctggagc cgcgcaggct
    24421 ttgccgcaac ctccacagtt gcgcggatcg ttggctacgt tggtgcagtc accactgcag
    24481 tcggtcttcg gggcttcgca caacgtaccg cacacaccgt ccgcgcagac ttggccggcg
    24541 gggcacgctt tgccgcacgc tccgcagtgg gcgacgctag atcgcgtgtc cacgcaagcg
    24601 ccatcgcaag acgtgagccc ctcgttgcag cgcgtgacac acatcgaacg ttcgcagctc
    24661 acggtggcgt ttggcttcga agcgcagacc gtgccgcatt ccccgcagtt ttgcaggtca
    24721 gtggtggtgt ccacacagcg gtctccgcac aggctgaagc cgctgttaca agtaattcca
    24781 catttgcctg ccgcgcatgc gggcttggcg ttgggcgcgc tggggcaagc cgtgccgcac
    24841 gcgccacagt tcgccggatc ggtcttcagg tctcgacata ccccgctaca atcggtttga
    24901 ctggtgttgc agctcgtctt gcacgcaccc ccggtacata cttggttttc gccgcaggcc
    24961 tgattgcagc caccgcagtt cttggggtca gaacgaagat cgatgcactg ctgcccacag
    25021 cgtacgaagc cggacccgca
//
LOCUS       DASTKU010000636        29905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1908853, whole genome shotgun sequence.
ACCESSION   DASTKU010000636 DASTKU010000000
VERSION     DASTKU010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 29905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29905
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908853"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFQ61_22200"
     CDS             <1..585
                     /locus_tag="VFQ61_22200"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET9957233.1"
                     /translation="VFCVRRSSDAGSAVDSEENESLTRALRQHLASSLPDYMLPSQIV
                     WLRSLPHTPSGKVDRMALPEVSRVVRAPECGSELQRQAARIWADILEAGEVSVDDDFF
                     ALGGHSILAMRLVSRIRAELEVELPLAVIFQKTTLGEQVMELALRKSRAEVAQTSEVE
                     VSAIGSAAEHALRELEELSDEEVARLLGSTSEDA"
     gene            689..13942
                     /locus_tag="VFQ61_22205"
     CDS             689..13942
                     /locus_tag="VFQ61_22205"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9957234.1"
                     /translation="MVDWSEQYRGLSQERRTAFALLLKKRGIDVLDVLPIQAVPRDET
                     APLSPAQERLWFLANLQPESAAYNIGEAILLRGKLDRAALEHALTELVRRHEVLRTSY
                     ESRDGKPVARVHAAVELALDSSALDDGLPADARRAMARELAECEAARPFDLGCAPLFR
                     VRLCQLAGGPDIQEWLLLWTMHHIISDGWSMSSMIGEFAQLYAAYGRERPAPLPVCAI
                     QYIDFAVWQREWLAGERGEKQLQFWLSELSDLPAALELPIDFQRGPALDTAGAVETRR
                     LDFELSEALRVAARASGVTPFMFLLGAFELLLARLTGQSEFCIGLPIANRSRPELENV
                     IGFFVNTVVVRARVAMAYTFAELLERVKSAMVNIQGNADVPFERVVERLRPERSLGRN
                     PIFDVLFNYHLDTLRGLEGLPGLEVEVLPRGSRSAQFDLSLDISQDDSGRLALGFGYR
                     TALFSRDTILRFATYFERILRRVVEQPQVRIGEVGMLDGSEQARLLDSALSAAVASAA
                     VVSADARAIPQLVPAWLQASMAARGNREALVCGGERLTFDELWARSGVVADALLHAGL
                     RPEQRVGVLVRRSTGLVVALIGVLRAGGAFVPLDPKYPSERLEFILNDSRIEYVITEG
                     QSQIDRAPSVYRVSLETALHRELDWRALEGPVASVHPRQAAYVIYTSGSTGVPKGVVV
                     EHGALAEHCAAIGAEFALDLTQSMLLFASVNFDAAIDQWLVPLLHGARVVMRGEEIWS
                     FEQMYDTIVRERITVLNILPSYLLEFAGWAELQGHRLPLESCISGGEALPMNGVALCR
                     RVLPTSARILNAYGPTETVVTPMVWHASSERVDQTGYAPIGRTLASRTGYILDSDLNL
                     LPERSTGELFLGGSVLARGYQGRPGLTAERFIPDPFGAPGARLYRTGDRVRRRADGTV
                     EYLGRTDHQVKLRGYRIELGEIEAELLQCPNVREACVLLLDAPKRLVGYVLTDGDVTS
                     EPELRTSLAQRLPEYMVPARLVMLRSFPRTPNGKTDKPALRSLASTQGLAATPLGDQP
                     EVELTDNQRTLLNIWRELLGVHAGVDDNFFELGGDSILTLQLVSRARGSGLMLRPRDV
                     FAHQTVRGLSSVCEERTEQSRDVNPRASAGPLGLTPIQRAFFEQRLPKPDHFNQAVLL
                     RASKRIDTSALRTALEAVLVGHDSLRLRFVEREGGIEQSYAEASPSVDDVLWQRSLSH
                     ANELEPLCDAAQASLCLRDGVLIRAVLARLPDGSDRLLIAVHHLAVDGVSFRILLEDL
                     ARVYGVALEGKPVEPIARTDSYREWSGRLEEYARSARVQAQIPFWQTVLAHCDGELPF
                     PSGDAEMERADESSHAREPELVRCQVAAGLTQQLLREANAAYRTQIQDLLLAAFARAV
                     GRLGAKSEFVIELEGHGREDLFDNLDVSRTVGWFTSIYPVHLPCQGDWKTVVTTIKGR
                     LRAVPDRGLGFGALRYLEGTLAGAPRSRIAFNYLGQFDAVFGNPAEAFAPARESAGRD
                     SARENPNEHWLEVNGQIFDGCLDLSFAFRPDRVPRSSVASLVAAFRQELESLLEHCLE
                     CPGAVTRSDFPLCSLDASELETLPVPPREIEDLYPLTPMQQGMLFHSLAARGAASQRA
                     LPDPYVNQSVFVVADLDVSRFENAWNQVIANHAVLRTRIVWEGLTDEPLQLVQRNVRL
                     QIVGYSAQDRLDAERTLLRVCDDDRRELGFAVAPLMRVSVIAEVDGPFRIVWTCHHLM
                     LDGWSSSRVIGEVIQQYVGVSSSRPRAEYRNYVAWLARRELASSEQFFRAELRKLSQP
                     TLLASACTERGSGPHRVLTKRLTGASRQHIVRFCQRERVTLNTLVQGAWAILLARVTG
                     QNVVALGSTVSGRPADLPNASEMVGMFINTLPLVVEMHPEQAPGDFLRDLQRRCVESR
                     EHEHTPLWVVQKLAPFATDHHGARGLFDTLVVVENYPLDRALSEHRGDLRILETRSSE
                     TTSYDMTLAVVDSGTNGGELTLNWDFSPEQYGPAWVERLAARFEALLLRLTRARAVGD
                     CGLATDSEIDALTRAATRNEGEDERPAQRSTHLDVVSRLAEVARWRGDADAVVAGLER
                     LSYSELARRAERIARRLVARGVQRESRVGVMLERSVDAIAALWGVWLAGAAYVPLDPA
                     YPRERLEYMLADCGARVLLTREELALDLTLPAGVVTEWADARGADADLQLPDRDTLSA
                     RQLAYVIYTSGSSGRPKGVAVEHGALAMHCFAAGALYGMGESDAALHFASLSFDAAVE
                     QWLVPLLHGAKLVLSQGLWTGEETRSVVLEEGITVIYPPTSYVTELARLVIGNSEQLP
                     IRVVTVGGEAVPRETIDLLQRAFGAQWVINGYGPTESVITPLLWRASRTASVGTAYAP
                     IGTAVGERSAYVLDSDGNLAPSDMPGELYIGGFGLARGYHERPSLTGSRFVPDPFGAP
                     GSRLYRTGDTCRRLTDGTFEYLGRVDEQVKIRGFRVELGEIEAELLSHDGVTDARVIL
                     RETERGKQLCAYVVSDEAGLSVEQLGLALARRLPEHMRPSHIVRLEKLPRTPNGKIDI
                     RALPQPEPVVREYRAVQTETERMLVEIWRSVLGVKEVGVQNNFFELGGDSILSIQIAS
                     RARRAALNISPRDVFEAQTVEELARRAEAAGRAVQCARGPLDVTLIDGAPFPLSPIQS
                     DFFARGIPEQHHFNQSLMLRCGREIDLCALGRALQHLVSHHDALRLRFEMRGGPTTQH
                     YAGSTVEPGAAGPGFARESQDLLWVNETSPEGLLELCQNVQRSLNLEAGPVLRAAHFK
                     LPDGSARLLLVIHHLVVDAVSWRILIEDLGQAYAAFARGAEPDLPSETSSYAAFTVAL
                     ERYANSETVASQLPYWRDVVSAVAASTVTCASRSARTKHVRLRVDARLTEALLRRAPV
                     TYRTQIQELLLTALGRALRRLTGASALAVEIEGHGREEALLQLDLSRTVGWFTTLYPV
                     VLPIAESIGECLMAIKETLRAVPHRGLGFGALTSYTQNPDLNQYVAQLRAAPRPSITF
                     NYLGQFDNTFQVPSEEAALFSPAEEDSGDESSRDAPPTNAVVINARVYGGELEIGFIC
                     ALDAQLAEHIEQLVEQYRRELEAIAAHCATAKPQWTPSDFALLAKTDHHSGLRADVNA
                     HVTLTQANIDDLAERYPDLEDIWPLTPMQEGMLFHALEAAPGADPYLNQLHVSVVDLD
                     VPRFVEVFRQVVAAHESLRVAFAWRGLPRPVQVVRRATQVSVEERWLGSDAELESTRE
                     REQLRGVDLEQGPLLRLVVARQPKGVTHLIWVNHHLVIDGWSSARLLEEVLGRYAGAE
                     SKPGRGRYRDYLSWLMQRDPRANERYFRALLAPLSAPTHLASALAGRPGGDGHGRLRW
                     RASPQETLRLQAFLQQQRLTANSLIQGAWALLLSRYCGQEHVVFGITVSGRPAEVPFS
                     EEALGLFINTVPVVTRVSSEMPIVDWLRTLIDQGAKSREHEHTPLYDVQRWSGCSGVP
                     LFDSLLVFDNYPVGEVLEQGFGGLKIGATTNREHTNYPLTIGIEMGSDLVVSLDYSRS
                     AVAEHAAEALLAQFSQLILTLAEDARRSVGQLGLPGGLITMDQPAKAGRHVLDALQEQ
                     VLYRGSSEAVICGETRVSFEELWRDAQVWAETLRALGVGAESRVGIAMERSARMLSGM
                     LGVWLAGGACVPLDGTHPVERLAHIVEDAVIQWVISDEQTAKRVAPSLRRVEWLTDAN
                     REPSATTVGSESFDLATRLHWGLAYVIYTSGSTGKPKGVLVDHGALACHIDAVTDKYG
                     YTEHDTALQFSSIQFDASLEQWLAPLSRGARVVLRGPDIWTASELLCAIELERITVID
                     LLPAYLRELAELALQKGRDLPVRLCVVGGEALPSATWKILDRVLEKSRLVNGYGPTEA
                     IITPLTCELNPALRADDDDVRDCHFAPIGGPLPGRAAHVLDSDLNPVPCGIAGELYIG
                     GASLARGYAGKAALTAERFLPDPFSTGGARLYRTGDRVRWLENGVVEYLGRTDDQIKV
                     RGYRVELGEIEAVLTEHAAVREAAVCLRGSPERLVAYVVWSTALAGSRELLEYARARL
                     PAYQVPTEIVPLPSLPRTPVGKLDRRALPDVLQTPRSHVEPRDDEERKMRDIWKRVLG
                     IEELGVTENFFDLGGHSVLAIRLLSQVNGAFSGPPSHAPGAPREVRLSQLLAAPTSRE
                     LTELLSPEPVQRPRARSGWVPLNGSQAQAAPLFCFHAAGGSVFPYRKLAQWLESTCPV
                     LGLPYALGAAETSLAQLAASYALQVSVAQPVGPYRLFGWSLGGALAALVAAELERTGY
                     TIEFLGLADCYLDAFGDHDSSQDDAEIENSIRAARGEWMGALAEQQLVEHEIVESLAA
                     QREIEARVRGSRLAPVKIRPDCYWSADVGRVGLSKVERELEHSLHTTVATSQRFDQDH
                     RGLVESVELAKHVAERLKLEGSN"
     gene            13954..15030
                     /locus_tag="VFQ61_22210"
     CDS             13954..15030
                     /locus_tag="VFQ61_22210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9957235.1"
                     /translation="MNFQNFVSAAGQRVSFALAGDTYRILVEGLGSIHFRVARERTEG
                     HEPRSTARLFVLAKKSTSVPAEAALFRELARCYFEAEPGSSTVELTGDVDGAAKRELI
                     RGGWAEEIALGVSVARARLYQCPSLWLVPPVREPFPLLYTVSNGKRHPARRPPIEGTF
                     YRRHLPQLEASLSFRTLDFERDLPTFHRWMNEPRVHTFWELAGPLERHREYLANLAKD
                     PHVCPIVGCFDEQPFGYFEVYWAKEDRLSPYYDAGDYDRGIHVLVGESRFRGPQRVGA
                     WLSSLVHCLFLDDCRTRSVVAEPRADNSKMIRYFESVGFHERKRFDFPHKRAALMISH
                     RETFFDQFCPGAVAPTETKQEFEQ"
     gene            15027..16382
                     /locus_tag="VFQ61_22215"
     CDS             15027..16382
                     /locus_tag="VFQ61_22215"
                     /inference="COORDINATES: protein motif:HMM:NF024826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SidA/IucD/PvdA family monooxygenase"
                     /protein_id="HET9957236.1"
                     /translation="MNSERELDLAGIGVGPFNLSLAALLEPIPGIHARFFERKPNFDW
                     HPGMMLAGTRMQTSFLKDLVTPVDPSSRYSFLSYIVEQGRFYRFVNAEFARVPRLEFA
                     AYLRWAASRIAALRFASEAREVLCTDRGFELRFSSGEVVYSKHLAVGTGLRPNVPSWA
                     RDHLGTRCSHSNDYQVRDLDVTDRSVAVIGGGQSGAEIFLQLLSGVRGTAKRVSWVSR
                     RAGLEPLDETAFTNEYFTPDYVRHYHELPEERRRALAYAQKLTGDGVSPETLRDLSQA
                     LYSRDFLEHRRTPYRILTNREIRSMSGRHSHYVLGMRNFFNQTDEYLEADVVLLATGY
                     RYRLPSCLSPIAELLELDADGLPRLNEDYTARWNPPFRGDAAQHRIYMLNAGRHSHGV
                     PDSQLSLAAWRSAVIVNSLCGRKVYDTTPQPAPLEWVDEQGPMSRRSNSGEVELRAGR
                     A"
     gene            complement(16532..16861)
                     /locus_tag="VFQ61_22220"
     CDS             complement(16532..16861)
                     /locus_tag="VFQ61_22220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957237.1"
                     /translation="MEHSGRWLLGLGLLGMLGFVGSCLGLLLHPLPGDAYLVCISCFL
                     AFYLTSLFVGYIGESCPREPRTRTPPALVQFSSGRHEPDEPLELNSAGPWRWRAKQRP
                     SLWVVSR"
     gene            complement(16888..17103)
                     /locus_tag="VFQ61_22225"
     CDS             complement(16888..17103)
                     /locus_tag="VFQ61_22225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957238.1"
                     /translation="MSNSCHHITIAKNGVASVQRCTQCDCISIHLGPTTVRIDAQTLE
                     ALWSVLGEATAQLRAEHRPTQLQRGVA"
     gene            17465..20059
                     /gene="mxcH"
                     /locus_tag="VFQ61_22230"
     CDS             17465..20059
                     /gene="mxcH"
                     /locus_tag="VFQ61_22230"
                     /inference="COORDINATES: protein motif:HMM:NF038079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore myxochelin receptor
                     MxcH"
                     /protein_id="HET9957239.1"
                     /translation="MAAALLGATCALPSHARAQVDRSAVSQSGTRAELPPSTASSEAA
                     RSAAAPSRRDSGSVKPPELITGAPPEYPLRAVQQPELATVVLRLTIDRAGVVTEAACV
                     DSIGPEFDEAAREAALKFRFQPAERAGEAVASRILFPMHFRRPAGPPLASNSTSATPG
                     MEPPQGARAPDPPLVVPVTSTPAARKDIEVTVYGQQSEAQRLRQSAEAVHVIETARAK
                     RETADLGEVLARSQGVAIRRDGGLGSSTRFALNGMYDDQVRFFLDGVPLDVAGYAFGI
                     ANIPIAFVDRVEIYRGVVPIRLGADALGGAVNLITNRRYDTHASASYQTGSFGTHRLS
                     LHGRYRHYPSGLVLGGEAFTDFAKNNYLVDVKVPDETGAQAAVRVPRFHDRYRAYGAV
                     LEGGVVDRPWAKALLFRAFVSGYDKELQHNVIMSVPYGGVEYGERIYGLTGRYQASVG
                     KNMELEIVGSYGHRTIEFSDDENWVYNWYGQRVRRRLVPGEIEAKARDTSVWQHSGFG
                     RAIYKWQLSPRHALGASYTANWAHRTGDERIQVDPDARDPLTAARKLFTLVGGLDYQL
                     SLFDDRLSNVVFVKDYLYHANTEEPLPGGVFRERTSDTHSWGVGDSMRFRFTPWLYAK
                     MSYEYATRLPRPDEVFGNGVLVQANLELEPEVSHNANAGPRLELPKTPIGSLVVDLNG
                     FLRDSDRLIVLLGNDRYFTYQNVYHAGGVGLESAVNWVVPRRWLSLDGTLTWQDQRNV
                     SNTGTFKSFEGDRIPNRPYLFASWAARLRVQGLPGKRDTLEPYYYGRYVHGFYRGWES
                     SGDPRFKQVVASQITHSLGMTWTVDRTEHKVTSTLEFENILDTRVFDNFGVQRPGRGV
                     YLRVTADM"
     gene            20115..20522
                     /locus_tag="VFQ61_22235"
     CDS             20115..20522
                     /locus_tag="VFQ61_22235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957240.1"
                     /translation="MDDLAENVRTFVSLIERGETLAAIERFYAMDVCVFENRELARAG
                     RENCLTYERESLGRITGLPRFKVHRFAVNEAAGVVFLEYTVRFTASSGRPMRLDEVAV
                     QTWEGSLITQERFYYEGLVDEGDDEPKLVATSG"
     gene            20729..22129
                     /locus_tag="VFQ61_22240"
     CDS             20729..22129
                     /locus_tag="VFQ61_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HET9957241.1"
                     /translation="MDRLRGVGLGFRPALASDLLRQRGCVDFVEVVAEACLFNGAQRR
                     EVRALSELWPVVPHGVKLSLGSANGIDVARVRALGQLARELKSSVITEHVAFVRTLSR
                     EIGHLTPLPRTATALGVLERNLGVARAHLPDLPLLVENVAWTVRYPERGEPHFPEPEF
                     YSELVERTGVGMLLDLGNLYANAINAGLEPESELRRYPLHAVGMLHLAGGIFEDGFYF
                     DDHAHAVPDQVLALLETALAAVGDVPIVIERDALFPPFAELSGEVSQVRAMLRGRERL
                     PVHSSLSARRPTHSALQHPVLQPSELQQFESQQATLAELLTNLEFPNSSELAGFEAAE
                     LWRSRAILQRKRLEEALPLLARLGAYWRDLEPWAERVIAQCPRAPRLAAVTDALQIAS
                     RAQREFDSPEIAFAARVDALVLSARFIGEPGQALRPRHPPFVAHVRSPRGARLWVIKG
                     FGSSAQTRIVERNTAV"
     gene            22126..22854
                     /locus_tag="VFQ61_22245"
     CDS             22126..22854
                     /locus_tag="VFQ61_22245"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9957242.1"
                     /translation="MKDLDSTPLRELPPELKRQLLAFTEQLENKLGASLVALFVFGGA
                     ARGEYHVGSSDIDCAIVLDSATPELLIGLGNLLQLGRFQSRFESIILTLEEVQHAADA
                     FPIFYSDMRRCHVLLTGRDVLSDLQISDHHLRLRVEQELRDCQIHLRRAITDSMGESH
                     ALAGAVIRKVKQIRAPLRALAQLEGRSVADKMGEVLADAGRRFQLDVSPLSEPDRAPV
                     VSVGTLMELLSRSIEVVDRMSGEA"
     gene            22859..24010
                     /locus_tag="VFQ61_22250"
     CDS             22859..24010
                     /locus_tag="VFQ61_22250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957243.1"
                     /translation="MDLGIDIFPFNLPPGGPFLVFFVWFALIVWGGAMLSRHALRRPF
                     ERFFLERSSALGSGVPLYAANVGMPAPGWSASASGPSGTPGAGLLKGRIPVGEQLFAV
                     AQLKGGKAGVGDVMITQALGLGLLRPTANPAELVSLQPTTSLQPAPLITQPAQWYSVD
                     SAVRVPPGVMSRFRARLTLHSSSPLTPEKIHEQAEQLADELEDGLEQQLIADGLRRSK
                     SVISIWQNWVLVPGIVVLITAAVRIVLLAIHDRPFTNLLFAAAVAAFMFVVFSKVSSK
                     NCLRGQEYLSWLDAATYSIQTEVRNGRRTEPNEVSLVLAVGGAGALVGAAAFGLLTPL
                     LSPAFRPIASASYAGDGSGSSSSCSSSSSCGGGGGCGGGGGCGGGGGCS"
     gene            24001..25332
                     /locus_tag="VFQ61_22255"
     CDS             24001..25332
                     /locus_tag="VFQ61_22255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957244.1"
                     /translation="MQLRPASSEPARFEVLDALGGWFRLEAGEATLAGSIPLRAARAC
                     SPFLEGNAIGYRLVTDAVFRVQRRLGLWRVEVSVPEAVRRAQRANLPQIAVTMANGGA
                     TSGADAARRGLMTRLERELVWIDGVTSSIPKLILFTGLFVRALPDCELLLSSAGNRRS
                     VTYGVEPGWVRATELVPLLIELTYRGLGDMTWEITGEVASLLPLLRPMSLETRSLSAR
                     SNLGEAHARFFDTNYFAQKKRGPTRKYKSMITESGSARAEQAPSTIGEWVEIVSRPQT
                     ANVRAETIPESDCGAAHLVRNAVSFDARFDGQHVVVTCDPVEMREHANRIEAEWRALY
                     GELGDARGALLYFTKYATLHQAGEPFFFVKPPALVSTPPGTACLVEGLCGPEYDVLRG
                     VIRTDVFHAVPTVIRLNWPGKKIRVPKGMPLAWVVPFERSSPHPSYRRREL"
     gene            complement(25386..27113)
                     /locus_tag="VFQ61_22260"
     CDS             complement(25386..27113)
                     /locus_tag="VFQ61_22260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957245.1"
                     /translation="MQRSSLASACFILISFAISTPASAVDVTTRAFDNNRSGWNGAET
                     TFKNNNLATLHLLRTFNVDEKVEAQPLVYGNALYVFSMNNTVYRFDVNTGALLNSRHL
                     AEAIDPCVQPGQMDMWCVYHKWGIASTPVIDPATNSLYLVTFQRAPGSGDNGNREHHL
                     WTLDATTLANQQPDILIRGNADNGGKHFNVGTETPYQKLRAGLGLLTDAAGNKAVILA
                     FSMNGENPAGPGNGFVFAYDTRGLHHQAGFTNTPGVWCVTPNGGAGGIWMAGSAPAIA
                     GNNIYFTTGNGAIGNSNYGESFVKLAYTAGTAGSNGGKPSLALSDFWTAFDDNGRAFN
                     DEDLGASGVLLVPGFQSLVGGGKDGVLYNLNRTDLGHNNWAPHFNLPFVATYLPNAPN
                     GAAGLPTSTLADANWPIVNLDRNLHDGTPDGKMHHLHGTPVYLRMATHGNVYLWGENE
                     RVKVYNYNFTTQRIDAFRNQGTVYSSAGKPPPGGMPGGMLALSSNGTTAGSAVLWATI
                     PKNGDANQAVVQGSFVVFDATTIVGNQELKKLYSYDYNYYSKFAPPVVANGKAYVPTF
                     GNSVLQFGL"
     gene            27474..28496
                     /locus_tag="VFQ61_22265"
     CDS             27474..28496
                     /locus_tag="VFQ61_22265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957246.1"
                     /translation="MATHRKASAGLWLGAVAIQICVACGTARNPEPKSVAEVTPKLQE
                     PAATPHGKSGACGDGQWCVASGIPKAVTFSSIWGSSSNDIWLAGEAGTLVHWDGVQWT
                     QVESGTQNSLRALTGTSATDVWAAGLEGTLLHYDGSTWSGVQRNGEPWSPTSGPNTRP
                     IYALYAAQPQRLWAAGSGARYFDGTAWSEPHHGSHLPIMGLWSSGEADTWAVGFQGTI
                     TRWDGHHWERVGRDMGPNYLAVWGSARNDVWIVGSAGAALHWDGTGWSAIPSGTTNDL
                     HAVRGFNAKDVWAVGDRGTILHWEGSAWVASPSLSERGLLALWGASAADVWAVGENGI
                     VLRRQN"
     gene            28674..>29905
                     /locus_tag="VFQ61_22270"
     CDS             28674..>29905
                     /locus_tag="VFQ61_22270"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9957247.1"
                     /translation="MGFSVGSSSQEMERARGLARVARALAELEDQETALKEVCEQVRH
                     VLGTTASAINLLDEGGEFFHWSAQCGHPETFPLEPIPWQLFDAFAAGFEPILSETRPE
                     VVRADPRLAMFSVHGLQAFATIPVRRSGRLLGLITAASYGTTRAPFDASEAVWLQTVG
                     DMVVQEVLRQRALFAARESEARYKRIVSTIRDGVWAIDADQRTEYVNPQMADMLGYRV
                     EELIGNRAPELNESWPGAGYKRWADRQQGRAERYEVHFRRKDGKEVYASVSAAPLFDK
                     EGNFSGSLSVVTDITEQRKLELKLQQTQKLESLGILAGGIAHDFNNLLVAMLGNASLG
                     LEIVRDDSELVPIFIDIRSAAQRAAELTRQMLAYSGRGRFVVRDLELNALIRETANLI
                     GTVMPKNAELQLVLSPGLP"
BASE COUNT         5378 a         8803 c         9659 g         6065 t
ORIGIN      
        1 gtcttctgtg tacggcgctc gtcggatgca ggctctgcag tagactcgga agaaaacgag
       61 agcttgacgc gcgcactccg tcaacacctg gcctcctcac tgccagacta catgcttccg
      121 agtcagatcg tctggcttcg atcgctaccg cataccccgt ctggcaaagt ggaccgcatg
      181 gcgctgcccg aggtgtcgcg agtcgtccga gcgcccgagt gcgggtcgga gctccagcgt
      241 caagcggccc gcatttgggc ggatatcttg gaagccgggg aagtgagcgt ggacgacgac
      301 ttctttgcgt tgggtgggca ctccatcctg gccatgcgtc tcgtttctag gatccgcgcg
      361 gagctcgagg tcgaactgcc cctcgccgtg atcttccaga agacgacctt gggtgagcaa
      421 gtgatggagc tcgcgctccg caagagccgc gctgaggtcg cgcaaacgag cgaggtagaa
      481 gtctctgcga tcggcagtgc ggcagagcac gcgctgcgtg agctcgaaga gctgagtgac
      541 gaagaggtgg cgcgtctgct cggttcgacg agcgaggacg cgtaacatcg agtcgaacga
      601 catcgggatt cgcgtgacat cgactcccgt actctgattc taacttgtgt attgggtgag
      661 ctgcgcttcg ggcgcaagcg agtgagatat ggtcgactgg tctgagcaat atcgtggtct
      721 gtcacaggag cgtcggacgg ctttcgcttt gttgctgaag aagcgaggca tcgacgtgct
      781 cgatgttctg ccgattcagg cggtgccccg cgacgagacc gcgccgctct ctcccgcgca
      841 ggagcggctc tggttcctcg caaatctcca gccagagagc gctgcgtaca acatcgggga
      901 agccattctg ttgcgcggca agctcgatcg cgccgcgctc gaacacgcgc tcactgagct
      961 cgtgcggcgc cacgaggtgc tccgaacttc gtatgaatcg agagatggca agcctgttgc
     1021 acgggttcac gctgcagtag agttggccct cgattcgtcc gcgctggatg acggattgcc
     1081 tgccgacgcg cgccgagcga tggctcgtga gcttgccgag tgcgaggcgg cgcgaccctt
     1141 cgatctcggg tgcgccccat tgttccgcgt gcggctttgc cagcttgcgg gtgggccaga
     1201 tatccaggag tggctgctgc tctggactat gcaccacatc atctcggacg gatggtccat
     1261 gtcctcgatg attggcgaat tcgcgcagct ctacgccgca tacggccgag agcgtccggc
     1321 gcctttgccg gtctgcgcca tccaatacat cgattttgcg gtttggcagc gggagtggct
     1381 cgccggggaa cgaggggaga agcagctgca gttttggttg agcgagctgt cggacctgcc
     1441 cgccgcgctc gaacttccaa tcgactttca gcgcggtccc gctctggata ccgcaggtgc
     1501 ggtggagaca cgccgtctgg atttcgagct gagcgaggct ctgcgagttg ctgcgcgggc
     1561 gagcggcgtg acgcctttca tgtttctgtt gggcgccttc gagctgctgc tcgctcgact
     1621 aacgggacag tccgagttct gcattgggtt gccgattgca aaccgatcac gccccgagct
     1681 ggagaacgtc atcgggttct tcgtcaacac ggtcgtcgtg cgggccagag tagctatggc
     1741 ctacacgttc gccgagcttt tggagcgtgt gaagtcagcc atggtgaaca ttcaaggtaa
     1801 cgcagatgtt cctttcgaac gcgtcgtcga gcggttgcga cccgagcgta gcttgggccg
     1861 aaacccaatc ttcgatgtgc tcttcaacta tcacttggac actctgcgcg gtctggaggg
     1921 gttgccgggc ttggaagtcg aggtgctgcc ccgcggctcg cgcagcgcgc aattcgatct
     1981 gagcttggac attagccagg acgacagcgg acgacttgcg ctgggcttcg gctaccgcac
     2041 ggcgctcttc tctcgcgaca ctattctgcg ctttgctacg tacttcgagc gaatattgcg
     2101 tcgcgtggtc gagcagcctc aggtccggat cggtgaggtc gggatgctgg atggatctga
     2161 gcaagcgcga ctgctggact ctgctctgag tgccgcggtc gcgagtgctg ctgtcgtgag
     2221 cgccgatgcg cgggctatcc cgcagctggt tcccgcttgg ctacaggcga gcatggccgc
     2281 tcggggcaat cgcgaagcct tggtctgcgg cggcgaacga ctcactttcg acgagctttg
     2341 ggctcgctcg ggggtcgtgg cggacgcgct gcttcacgcc ggtttgcgcc ctgagcagcg
     2401 cgtcggtgtg ctcgtgcgtc gttcgacggg gctcgtggtt gccttgattg gagtgttgag
     2461 ggcgggaggc gctttcgttc ctttggatcc aaagtatccg agcgaacgtc tagagttcat
     2521 actcaatgat tcacgaatcg aatacgtgat taccgagggc cagagccaga tcgatagggc
     2581 gcccagcgtc taccgcgtct ctctcgagac ggcgctccac cgcgagctcg attggagagc
     2641 gctagaaggg cccgtcgcga gcgtacatcc acgccaagcc gcttacgtaa tctacacgtc
     2701 aggctcgacc ggcgttccca agggcgtcgt cgtggaacac ggcgctcttg ccgagcattg
     2761 cgcggccatc ggcgccgagt tcgcgctcga tctaacgcaa tcgatgctgc tcttcgcgtc
     2821 ggtcaacttc gacgcggcga tcgatcagtg gctcgtaccg ctcttgcacg gggcgcgcgt
     2881 ggtgatgcgc ggtgaggaaa tttggagctt cgaacagatg tacgacacga tcgtgcgcga
     2941 gcggattacg gtcctgaata tcctgccctc ttatttgttg gagttcgccg gctgggcgga
     3001 actacagggt catcgactac cgcttgagtc ctgcatatcc ggcggcgagg cgctgcccat
     3061 gaacggggtg gccctatgcc gtcgtgtttt gccgacgagc gcccggatcc tcaacgccta
     3121 cgggccgacc gaaacggttg tcacaccgat ggtgtggcac gccagctccg agcgagtgga
     3181 ccagactgga tatgccccga ttgggcgaac gctcgcatca cgcaccggct acattctcga
     3241 ctctgatttg aacctcttgc ccgagcggag caccggagaa ctattcctgg gtggctcggt
     3301 tttggcccga ggctatcagg ggcggcctgg gctcacggca gaacggttca ttcccgatcc
     3361 gtttggcgcc cctggcgcac gactgtatcg cactggtgat cgtgtacgcc gacgcgcgga
     3421 cggaaccgtc gagtacctgg ggcgaacgga tcatcaagtc aagttgcgcg ggtaccggat
     3481 cgagctcggc gaaatcgagg ccgagctcct gcagtgcccc aacgtgcgag aggcgtgcgt
     3541 gttgctgctc gatgctccga agcgtctcgt gggatatgtc ctgactgatg gtgatgtgac
     3601 gagcgaaccg gagctgcgca cgtcgctggc gcagcgactg ccggagtata tggttccagc
     3661 gcgtttggtc atgttgcgga gctttcccag gactcccaac ggcaaaaccg acaaacccgc
     3721 actgcgctcg ctggcgtcga cacagggttt ggccgcgacg ccgctcggcg atcaacccga
     3781 ggtggagctc acggataacc agcgcaccct attgaacatt tggcgcgagc tcctaggcgt
     3841 tcacgcgggc gtcgacgaca acttcttcga gctgggtgga gattcgattc tgactctcca
     3901 gctcgtgagt cgagcccgcg gctcggggtt gatgctgcgt cctcgcgacg tttttgcgca
     3961 tcaaaccgtg cgtggcttga gctctgtttg cgaagagcgg actgaacaat cccgggatgt
     4021 caatccgcgc gcgtcggcgg gcccgctcgg gctgacgccc attcagcgcg ctttcttcga
     4081 gcagcgttta ccaaagcccg atcacttcaa tcaggccgtg ctcttgcggg cctccaagcg
     4141 gatcgacacg agcgcgttgc gtacggcgct ggaagcggtt ttggtaggcc acgatagcct
     4201 gcgcttacgc ttcgtcgaac gcgagggggg aatcgagcag agctacgcgg aagcgtcgcc
     4261 ctcggtcgat gacgtcctgt ggcagcgctc tctgtctcat gcgaacgagc tcgagcctct
     4321 ctgcgacgcg gcccaagcga gcctgtgttt gagagacggt gtgttgatcc gcgccgtgct
     4381 cgcgaggtta ccggacggga gcgaccgact actgatagct gtgcaccacc tcgccgtgga
     4441 cggcgtctcg ttccgaatct tgctcgagga cttggcgcgt gtctatgggg ttgctctcga
     4501 aggcaagcct gtcgagccga tcgcccggac cgattcctac cgcgagtggt caggtcgact
     4561 cgaggagtac gctcgcagtg cgcgcgtgca ggcgcaaatc ccgttttggc agaccgtact
     4621 cgcacattgt gatggcgagc tcccgttccc aagcggggat gccgagatgg aaagagcgga
     4681 cgaatccagc cacgcacgcg aaccggagct cgtgcgatgc caagtcgcgg ccggactcac
     4741 ccagcaactc ttgcgcgagg caaatgccgc ctatcggact cagattcaag atcttctgct
     4801 cgccgcgttc gcccgcgccg tcgggcgtct cggggccaaa tcggagtttg ttatcgagct
     4861 cgaaggtcat ggtagggagg acttgttcga taacctcgat gtgagtcgca cggtcgggtg
     4921 gttcacgtcg atctacccgg ttcacctgcc ttgccaagga gactggaaga cagtggtgac
     4981 cacgattaag gggcgtctgc gcgctgttcc cgaccgcggc ctcgggttcg gggctctccg
     5041 ttatctcgag ggaacactgg cgggagcgcc acgatcccgg atcgcgttca attacctcgg
     5101 tcagttcgat gcggtgtttg gcaaccccgc ggaggccttc gcgcctgcgc gcgagagcgc
     5161 gggccgcgat agcgctcgcg agaatcccaa cgagcactgg ctcgaggtca acggacagat
     5221 cttcgacggt tgcctcgatc tgagcttcgc gttccgcccg gatcgcgtgc cccgcagcag
     5281 cgtagcgtca ttggttgcgg ctttccggca agagctcgag tcgctgctcg agcattgttt
     5341 ggaatgccca ggcgcggtga cgcgctctga cttcccgctc tgtagcttgg acgcgagcga
     5401 gctagagact ttgcccgttc cgccccggga gatagaagat ctgtacccgc tcacgccgat
     5461 gcagcaggga atgctgtttc attcgctggc cgcgcgtggt gcggcaagtc aacgcgcgct
     5521 tcccgacccg tacgtgaacc aaagcgtctt cgtggtggct gatttggatg tgtcgcgctt
     5581 cgagaatgct tggaaccaag taatcgcgaa tcacgccgtc ctgcgaacgc gcatcgtttg
     5641 ggagggcctt acggatgagc cactccagct cgttcagcgg aatgtccgct tgcagatcgt
     5701 gggctactcc gcccaggatc gcctcgacgc ggagcgcacg ctccttcgag tgtgcgatga
     5761 cgatcggcgg gagctcggat ttgcggtggc gccgctgatg cgggtgagtg tcattgccga
     5821 ggtggacggg ccgttccgta tcgtgtggac ctgccaccac ctgatgctgg acggttggag
     5881 tagctcccgg gtgatcggcg aggtgattca gcagtatgtg ggagtctcca gctcgagacc
     5941 gcgcgcagag tataggaatt acgtagcctg gctcgcgagg cgcgagctcg cctcgagtga
     6001 gcagttcttc agagcggaac ttcggaagct gtcgcagccc acactgttgg cgagcgcctg
     6061 caccgagagg ggtagtggtc cgcaccgtgt gctcacgaaa cgactgacgg gagcatcgcg
     6121 tcagcatatc gtacggttct gccaacgtga gcgggtgacg ttgaatacgc tggtccaggg
     6181 ggcatgggcc attctgctcg cacgcgtgac gggccaaaac gtagtggcct taggcagcac
     6241 cgtttccggt aggccggcag atttacccaa tgcctccgag atggttggca tgttcatcaa
     6301 cacgctgccg ctggtggtgg agatgcatcc ggagcaggcc ccgggtgact tcttgcggga
     6361 ccttcagcgt cgctgcgtgg agtcacgcga gcacgagcat actcctctct gggtcgtgca
     6421 gaagcttgct ccattcgcca ccgaccacca cggcgcgcgc ggcttgttcg atactctggt
     6481 cgtggtcgag aattacccgc tggatagagc gctctcggag caccgcggcg acctgcgcat
     6541 tttggagacg cggagttcag aaacgacgag ctacgatatg acgctcgcgg tggtggattc
     6601 ggggaccaac ggtggtgaac tcaccctgaa ctgggacttt tcgcccgagc agtacgggcc
     6661 ggcttgggtg gaacgactgg ccgcgcgttt cgaagcgctg ttgcttcgtc tcacacgagc
     6721 gcgggcagta ggggattgcg gtttggcgac agactccgaa atcgacgcat tgacccgagc
     6781 tgcgacgcgg aacgaaggag aagacgagcg accagctcag agatccactc acctcgatgt
     6841 cgtaagtcgg cttgctgagg tcgctcgatg gcggggagat gcggacgcag tggtggctgg
     6901 tttggagcgg ctcagctact ccgagctcgc gcgccgcgcg gagcgcatcg cgcgtcgttt
     6961 ggtcgcgcga ggtgtgcagc gcgagtcacg agtcggggtg atgctcgagc gcagcgtcga
     7021 cgcgatcgct gcgctgtggg gcgtttggct ggccggcgcc gcctacgtgc cgctcgatcc
     7081 ggcgtatccg cgagaacgtc tcgaatacat gctcgccgat tgcggggctc gcgtgttgct
     7141 cacgcgcgaa gagctggccc tcgatctcac tctcccggcg ggcgtggtga ccgagtgggc
     7201 ggacgctcgc ggggctgatg ccgatctgca gttgcccgat cgggacacgc tgtctgctcg
     7261 acaactcgct tacgtaatct atacatccgg gtcgagcggg cggcccaagg gcgtcgccgt
     7321 cgaacatggt gcgctcgcca tgcactgctt cgcggcggga gcgctgtacg ggatggggga
     7381 gtcggacgct gcgttgcact tcgcctccct gagcttcgac gcagcggtcg agcaatggct
     7441 cgtgcctctg ctacacgggg ccaagctggt tttgagccaa ggactgtgga cgggtgaaga
     7501 gacacggagt gtagttctcg aggaggggat taccgtgatc tacccgccca cgagctatgt
     7561 gaccgagttg gcacggttag tcatcgggaa ctcggagcaa ctcccgattc gcgtggtgac
     7621 ggttgggggt gaagcggtcc cccgagagac gattgatcta ttgcagcgcg cctttggggc
     7681 gcagtgggtg atcaatggct atggccctac cgaatcagtc attaccccgc tactctggcg
     7741 cgcatcccga acggcctcgg tgggcacggc ttacgcgccg attggaaccg ccgtgggtga
     7801 acgcagcgcc tatgtgctcg attcagatgg taacctcgcg ccatccgata tgcccggcga
     7861 gctatatatc ggagggtttg ggcttgcacg cggctatcac gagcggccga gtctgaccgg
     7921 cagccgcttc gttccggatc cgttcggcgc tcccggcagt cggctgtatc gaaccgggga
     7981 tacttgccga cggctgacgg acggtacttt cgaatatcta ggtcgtgtcg acgagcaggt
     8041 aaagattcga ggctttcgcg tcgaactcgg tgagatcgag gcagagttat tgagccatga
     8101 tggcgtgacc gacgcgcgtg tcatcctgcg tgagacggag cgtggtaaac agctgtgcgc
     8161 ttacgtcgtg agtgacgagg cggggctcag cgtagagcaa ttggggttgg cgttggcgcg
     8221 ccgcctaccg gagcacatgc ggccaagtca catcgtgcgg ctcgagaagc tgcctcgaac
     8281 cccgaacggc aaaatcgaca tcagggcgct tccccagcca gaaccggtcg tgcgcgaata
     8341 ccgggccgtg caaaccgaga ccgagcggat gctggtcgag atttggcgca gtgtgctcgg
     8401 agtcaaggaa gttggcgtgc agaacaactt cttcgagctg ggcggggact cgattctatc
     8461 cattcagatt gcaagccgtg cacgtcgtgc ggctctgaac attagtcctc gcgacgtgtt
     8521 cgaagcgcag accgtcgaag aactcgcgcg tcgtgcggag gccgcgggcc gtgcggtgca
     8581 gtgtgcgcgt ggtcctctcg acgtcacgct catagacggc gcacctttcc cgctttctcc
     8641 gattcaatcg gacttctttg ctcgtgggat ccccgagcaa catcacttca atcaatcact
     8701 catgttgcgc tgcgggcgtg agatcgacct gtgcgcgctc ggtcgcgccc tgcaacatct
     8761 cgtctcgcat cacgatgcgt tgcgcttgcg cttcgaaatg cgcggcgggc ccaccacgca
     8821 acactacgca ggctcaacgg tagagcccgg ggcggcgggg cccggcttcg ctcgagaatc
     8881 gcaagacctg ctctgggtga atgagaccag cccggagggg ttgctcgagc tatgccagaa
     8941 cgtgcagcgc agcctgaacc tcgaagcggg cccagtgttg cgagccgctc atttcaagct
     9001 cccggacggt tccgcgcgct tgctgctcgt cattcaccac ttggtcgtgg acgcggtttc
     9061 atggcgaata ttgatcgaag acctgggaca agcgtacgct gctttcgcgc ggggggctga
     9121 gccagacttg ccctctgaaa cctcctccta cgcagcgttt acggtcgcac tcgagcgcta
     9181 cgcgaactcg gagacggtgg cttcacagct cccttattgg cgtgatgtcg tcagtgcagt
     9241 cgccgccagc acggtcacgt gcgcttctcg ttctgcgagg accaagcacg tgcgtcttcg
     9301 ggtggacgcc aggttgaccg aggccttgtt gcggcgagca ccggtcacct atcgaacaca
     9361 gatccaagag ttgctgctga ccgcactggg acgcgctctc cggcggctca cgggcgcctc
     9421 ggctctcgcc gtcgagattg aagggcacgg acgcgaagaa gctctcttac agctggacct
     9481 gagtcgcacc gtaggctggt tcaccacgct atatccggtg gtgctcccca ttgcggaaag
     9541 catcggcgaa tgtctaatgg cgatcaagga gacactgcga gccgtgcccc accgagggct
     9601 gggattcggc gcgctcacgt cctacaccca gaatcctgat ctgaaccagt atgttgcgca
     9661 gctcagggcg gcgccgcgcc cgagtatcac cttcaactat ctgggccaat tcgataacac
     9721 gttccaagtt ccgtccgagg aagccgcgct attttcaccg gcggaggaag actccgggga
     9781 cgaatcgtct cgtgacgcac ccccaaccaa cgcggtggta atcaacgctc gggtgtatgg
     9841 cggtgaactg gagattggct tcatttgcgc gctggacgcg cagctcgcgg agcacatcga
     9901 gcagctcgtg gagcagtacc gacgggagct cgaagccatc gcggcccatt gcgccacggc
     9961 aaagccgcaa tggactccgt cggacttcgc actgctcgcg aagacagacc accactcggg
    10021 acttcgcgcc gacgtgaatg ctcacgtgac gctcactcag gcgaatatcg atgatttggc
    10081 agaacgctac ccagatctag aagacatatg gccactcacg cccatgcagg aaggcatgct
    10141 gttccacgcg ctcgaagcgg ctccaggcgc ggacccctat ctcaaccaac ttcacgtctc
    10201 ggtcgtcgat ctcgatgtcc cccgcttcgt agaggtgttc cgccaagtag tcgccgcgca
    10261 cgagagcttg cgtgtcgcct tcgcgtggcg cggcttgcct cgacccgttc aagtggtgcg
    10321 gcgagcgacg caggtcagcg tggaggagcg ctggttaggg tcggatgccg aactcgagtc
    10381 cactcgagag cgagaacagc tacggggagt ggacttggaa caaggaccgc tgttgcgcct
    10441 ggtcgttgct cggcagccaa agggcgtcac ccacctcatc tgggtcaatc accatctcgt
    10501 catcgacggc tggagcagcg ccagattgct ggaggaggtg ctcggtcgtt acgccggtgc
    10561 cgagtccaag cccggtcgcg gacgctacag ggactatctc tcgtggttga tgcagcgcga
    10621 tccgcgcgca aatgagcgct acttccgcgc gctcctcgca ccgctgagtg cgccgaccca
    10681 tttggcttct gcgctcgctg gtcgccccgg tggtgatggc cacggccggt tgcgctggcg
    10741 ggcgtcaccc caggagacgc tccggctgca ggcgtttctc cagcaacagc gcctgaccgc
    10801 gaatagccta atacaaggcg cgtgggcgct gctcttgtcg cgctactgtg gtcaagaaca
    10861 cgtggtgttc gggatcaccg tatccggacg ccctgccgag gttccgttca gcgaagaagc
    10921 tctgggctta ttcatcaaca ccgttcccgt cgtgacgcgc gtaagcagcg aaatgccgat
    10981 agtggattgg ctgcggaccc tgatcgacca aggcgcgaag agccgggaac acgaacatac
    11041 gcctctttac gacgttcagc gctggtccgg ctgctctggg gttccgctgt tcgactcgtt
    11101 gctcgtattc gataactacc cggtcggcga ggtcctggag cagggctttg gaggactgaa
    11161 gatcggtgcc accacaaatc gggagcacac caattatccg ctcaccatcg ggatcgaaat
    11221 ggggtcggac ctcgtggtct ccctcgacta ctctcgaagc gcagtcgcgg agcatgcggc
    11281 cgaggcgttg ttggcgcagt tctcgcagct gattttgacg ctcgcagaag acgcgcgtcg
    11341 ctcggtcggg cagctaggtt tgccgggcgg gctcatcacg atggaccagc cggcgaaagc
    11401 gggccgtcac gttctcgatg ccctacagga gcaggtcctg taccgcggct cttccgaggc
    11461 ggtgatttgt ggtgaaactc gggtgagctt cgaggagcta tggcgtgacg cacaggtgtg
    11521 ggccgagacg ctgagagcgt tgggcgtggg agccgaatcg cgcgttggaa tcgccatgga
    11581 gcgctcggcg cggatgctga gcggcatgtt gggcgtgtgg ttggcaggcg gcgcttgcgt
    11641 ccccctcgat gggacgcatc ccgtcgagcg tctcgcgcac atcgtggaag acgcggtcat
    11701 tcaatgggtg atcagtgacg agcaaacggc aaagcgagtc gccccatcgc tccgccgcgt
    11761 ggaatggctt acggacgcca atcgcgagcc ctcggcaacc acggttggct ctgagtcctt
    11821 cgacctcgcc actcgtctcc actgggggct cgcctacgtg atctatacct cgggatcgac
    11881 gggtaaaccg aaaggcgtgc tcgtcgatca tggcgcgctc gcttgtcaca tcgacgcggt
    11941 taccgacaag tatggttaca cggagcacga tactgctctt cagttttcgt ccattcagtt
    12001 cgacgcctcg ctcgaacagt ggctggcgcc cttgtcacgc ggggctcggg tcgtgctgcg
    12061 gggccccgac atatggaccg cttcagagct cctgtgtgcc atcgagttag aacgcattac
    12121 cgtcatcgat ttgctgcccg cgtacctacg cgagctcgcg gagctcgcgc tacagaaggg
    12181 gcgggatttg cccgttcgat tgtgcgtcgt gggtggcgaa gccctacctt ccgcaacttg
    12241 gaagatcttg gaccgcgtgc tcgaaaagag ccgcttggtg aacggatacg gccccacgga
    12301 ggcgatcatt accccactca cctgcgagct gaacccagca ctcagagcag atgatgacga
    12361 cgttcgtgat tgccactttg cccccatcgg aggtcctttg ccgggccgcg ccgcgcacgt
    12421 tttggattcg gatctgaatc ccgttccgtg cggcattgct ggcgaactct acatcggtgg
    12481 ggcgagtctg gcgcgtggct acgctggcaa ggctgctctc accgccgagc gcttccttcc
    12541 cgatccgttc tcgaccggcg gcgcgcggct gtatcgtacg ggagatcggg tgcgctggtt
    12601 ggagaacgga gtggtcgagt atctggggcg tacagacgat cagatcaaag tgcgcggcta
    12661 ccgggtggag ctcggtgaga tcgaggccgt gctcaccgaa catgctgccg tccgtgaagc
    12721 tgccgtttgc ctacgcggaa gccccgagcg gctggtcgct tatgtcgtct ggtcgactgc
    12781 gttggccggc agccgcgagc tgctcgagta cgcgcgggcg cgcctgcccg cgtaccaggt
    12841 gccgacggag attgttcctc tgccgagcct accgagaaca ccggtaggca agctggaccg
    12901 tcgcgcgctc cccgacgttc ttcagacacc acgcagccac gtcgagcccc gcgatgacga
    12961 agaacggaag atgcgcgaca tttggaaacg agtattgggt atcgaggagc tcggcgtaac
    13021 cgagaacttc ttcgatctcg gtggtcattc ggttctcgcc attcggctac tttcccaagt
    13081 gaacggggcg ttttccggcc cgccctcgca tgctcccggc gccccgcgtg aagttcggtt
    13141 gtcgcagctg ctcgccgcgc caaccagtag agagttgacc gagctcttgt cgccagagcc
    13201 tgtgcagcgg cctagagccc gcagcggctg ggtgccgctg aatggatcgc aagcgcaagc
    13261 cgcgccgctg ttctgctttc atgcggcggg cggctccgtc tttccctatc ggaagctcgc
    13321 gcaatggctc gagtcgacat gtcccgtgct cggcttgcca tatgcactcg gtgccgcgga
    13381 gacgagcctc gcacagcttg ccgcgtccta cgccttgcaa gtctcggtcg cgcagccggt
    13441 tggcccgtac cgcttgtttg gttggtcctt gggcggcgca ctcgctgcgc tggtggccgc
    13501 cgaactcgag cgcactgggt acacgattga attcctcgga ctggccgatt gctacctcga
    13561 tgcgttcgga gaccacgatt cttcgcagga cgatgccgaa atcgagaaca gcatccgggc
    13621 agctcgggga gaatggatgg gcgcgctcgc ggagcaacag ctagtcgagc acgagattgt
    13681 cgagtcactc gcagcgcagc gagagatcga agcccgagtg cgggggagcc ggctagcgcc
    13741 cgtgaagatt aggcccgact gttactggtc ggcggacgtg ggtcgagtcg gactgtccaa
    13801 agtggagcgc gaactcgagc actcgctcca cactaccgtc gcgacgagcc agcggttcga
    13861 tcaggatcat cgcggcctag tcgaatccgt cgagttggcg aagcacgttg cggagcgact
    13921 caagcttgaa ggctctaact aagtattacg cacatgaact tccaaaattt cgtcagtgcg
    13981 gcagggcaac gggtctcatt cgctctcgct ggagatacct atcgaattct cgtcgagggc
    14041 ctcggttcga ttcactttcg tgtcgctcgg gagcgaaccg aaggccatga gccacggagc
    14101 acggcgcggc ttttcgtact cgccaagaag tcgacgtccg tccccgccga agcggcactc
    14161 tttcgcgagc tcgcacgctg ctacttcgag gctgagccgg ggtctagcac ggtggaactg
    14221 actggagacg tcgacggtgc ggccaagcgc gagttgatcc gcggcggttg ggcggaggag
    14281 atcgccttgg gcgtgagcgt ggcgcgcgca agactctacc agtgcccctc actctggctg
    14341 gtacccccag tgcgcgagcc ctttcccctg ctctacacgg tgtcgaatgg caagcgccat
    14401 cccgcacgca ggccgccgat cgagggcacg ttctatcgca ggcatctgcc gcagctcgaa
    14461 gcgagtctct cgttccgaac cctcgacttc gagcgcgacc tacccacatt ccaccgctgg
    14521 atgaacgagc cgcgagtcca tacgttttgg gagctcgcgg ggcctctcga gcgccaccgt
    14581 gaataccttg ccaacctcgc gaaagatccg cacgtgtgcc caatcgtggg ttgcttcgat
    14641 gagcagcctt tcggatattt cgaagtctac tgggcgaagg aggaccgcct ctcgccgtac
    14701 tacgatgcag gcgactacga ccgaggtatt catgtgttgg taggagagtc gcggttccgc
    14761 ggtccgcagc gcgtcggagc ctggctctcg tcgctcgtac actgcttgtt tctcgacgac
    14821 tgcagaactc ggagcgtcgt cgcggagccg cgcgcagaca actcgaagat gatccgctac
    14881 ttcgagagtg ttggattcca tgagcggaag agattcgact tcccgcacaa gcgggcggcg
    14941 ctaatgatca gtcaccgtga gacgttcttc gaccaatttt gtccgggtgc agtcgccccg
    15001 accgaaacaa aacaggagtt cgagcaatga acagcgaaag agaactggat ttggcgggca
    15061 ttggtgtggg gcccttcaac ttgagtctgg ccgcactgct cgagccgata cccggtattc
    15121 atgcgcgctt cttcgagcgt aaaccgaact tcgactggca tcccggtatg atgctcgcgg
    15181 gcacccgtat gcagacgtcc ttcctgaagg atctggtgac ccccgtagat ccttcgagtc
    15241 ggtacagttt cctttcctat atcgtggagc aggggaggtt ttatcgcttc gtgaatgcag
    15301 aattcgcgcg ggttccgcgc ctagaatttg cggcgtatct acgctgggcg gcgtctcgaa
    15361 tcgccgcgct ccgtttcgcc agcgaggcga gggaggtgct ttgcaccgat cgagggttcg
    15421 agctcaggtt ctcgtccggc gaggttgtgt acagcaagca tttggccgtg ggcaccggcc
    15481 ttcgacctaa tgttccgagc tgggcccgcg atcatctcgg aactcgctgc tctcactcga
    15541 atgactatca agtacgggat ttggacgtga ccgatcgaag cgtggccgtg attggcggtg
    15601 gccagagcgg cgcggagatc tttcttcaac tgctctcggg cgtgcgaggc acggcgaaac
    15661 gagtcagctg ggtctcgcgc cgcgcggggc tggagccttt ggacgaaacc gccttcacca
    15721 atgagtactt cacgccggat tacgtgcgtc actaccacga acttcccgag gagcgccgtc
    15781 gagccctagc gtacgcacag aaactcacgg gtgatggcgt ttctcccgaa acgctgcggg
    15841 acctcagcca agctctatat agccgtgact ttctcgagca ccgcagaacc ccataccgaa
    15901 tccttaccaa ccgagagatt cgaagcatgt ccggtcgcca cagccactac gtgttgggca
    15961 tgcgaaactt cttcaaccag accgacgagt acttggaagc cgacgtggtg ttgctggcaa
    16021 caggctaccg ctaccgactt ccaagctgct tgtccccgat cgcggagcta ttggagctcg
    16081 acgcggatgg gttgcctcga ctgaacgagg actacaccgc gcgttggaac ccacctttcc
    16141 ggggtgatgc ggctcagcat cgcatctaca tgctaaatgc tggtcggcac agccatggcg
    16201 tgccggactc gcagctcagc cttgccgcgt ggcgctccgc ggtcatcgtc aacagcttgt
    16261 gtggccggaa ggtctacgac accaccccgc agccagctcc gctggagtgg gtggatgaac
    16321 agggcccaat gtcacgccgc tcgaactctg gggaagtaga gctaagggcc gggcgcgctt
    16381 gagagccgcg tgtcgcgcag gggggaactt tcctcacgca atgttcggct cagaagtgct
    16441 ggcggcctcg gatcttcccc gacgctgccg tccgacagat gacaccgagc tgcaaggacg
    16501 gcgagctacg ccgcgaagcc cgccagcacc gtcatcgaga gacgacccaa agactcggcc
    16561 tttgctttgc ccgccagcgc cagggacccg cagagttcag ctcgagtggc tcgtcgggtt
    16621 cgtgtcgacc cgaactgaat tgaacgagcg ccggcggcgt ccgagtgcgt ggctcacgcg
    16681 ggcagctctc cccaatgtag cccacgaaca ggctcgtcag atagaaggcg aggaagcacg
    16741 agatgcacac caggtaagcg tcgccaggca acgggtggag gagcagcccc aagcagctac
    16801 ccacgaagcc aagcatgccg agcagcccca gccccaagag ccagcggccc gagtgctcca
    16861 tgcaccggcg acaccaccgg cgaaacatca tgcaacaccg cgctgcagct gcgtggggcg
    16921 gtgttcggct cgcaactgcg ccgtcgcctc tcccaaaacc gaccaaagag cttcgagcgt
    16981 ctgcgcgtcg attcgcactg tggttggtcc caaatgaatc gaaatgcagt cacattgcgt
    17041 gcagcgttgc acgctagcga caccgttctt ggcgatagta atgtgatggc acgagttcga
    17101 catgtgaatc ctctggcttc gtagattgat gagaatgatt cgcattgtca atcgtgaagg
    17161 ctggggccat gatcattcga tctttggacg gcagaggctg ctcgggtgcg cagcgcgcga
    17221 tgttgaggag gtatcggtca tggtccgggt cctagcgccg agctcgggtt tggccctcct
    17281 ggtccgaagc gggaagcaag atccagactg aggaggcttc tctcccctgg aactcggccg
    17341 aatctgcgct tgactctggt gggagtttgt gtgaatcgaa aacgcgactc acttgcagaa
    17401 tacagcgcta gccccagttt tgcccccaat caggcaatct cagagcagga gaaagctcct
    17461 ggtgatggcc gcggcgttgt tgggtgcgac ttgtgcatta ccgtcccacg cccgcgccca
    17521 agtggaccgg tctgccgtgt cacagtccgg aacacgagcc gagcttccac cgtccactgc
    17581 gtcgtctgaa gccgcgcggt ccgcagccgc gccgtcgcgg agagattcgg gctccgtcaa
    17641 accgccggag ctcatcaccg gagcaccacc ggaatacccg ctacgggctg tccagcagcc
    17701 ggagctcgcg acggttgttt tgcgactgac catcgatcgt gcgggggtgg tcaccgaagc
    17761 ggcctgtgtc gattccattg ggcccgagtt cgacgaagcc gcacgcgagg cggcactcaa
    17821 gtttcgtttt cagccagccg aacgagcggg agaggcagtt gcttcgcgga tcctcttccc
    17881 tatgcacttc cgacgccccg cggggccgcc cctagcatcc aactcgacct ctgcaactcc
    17941 gggtatggag ccaccccagg gcgcgagggc gcccgatcca ccactcgtgg tgcccgttac
    18001 ttcgactcca gctgcgagaa aagacatcga agtgacggtc tacggacagc aaagcgaggc
    18061 tcagcgcctt cgccaatctg cggaagcggt ccacgtcata gagaccgcga gagccaaacg
    18121 cgagacggcc gacctcggcg aggtgttggc acgcagtcag ggcgtagcca tacgtcgtga
    18181 tggtgggctc ggctcgagca cacgcttcgc gttgaatgga atgtacgacg atcaagtgcg
    18241 attctttcta gacggggtac cgctcgatgt tgcgggctat gccttcggaa tcgcgaatat
    18301 cccgattgct ttcgtggacc gtgtcgagat ttatcggggc gtcgtgccga ttcggctcgg
    18361 cgccgacgcg ctcgggggcg ccgtcaattt gattacgaat cgacgctacg atacccacgc
    18421 ctccgccagc taccaaactg gttctttcgg aacccaccgg cttagtctcc atggccgcta
    18481 ccggcactac ccttctggac ttgtcttggg aggtgaagca ttcaccgact tcgccaagaa
    18541 caactatcta gtggacgtga aggtgccaga cgagacgggg gctcaggctg ccgttcgggt
    18601 gccccgcttc cacgacaggt atcgagctta cggcgccgtc ctcgagggag gcgtcgtcga
    18661 tcgcccctgg gccaaggcgc tcctctttcg ggccttcgta tctggatacg acaaggagct
    18721 gcaacacaac gtcattatgt cggtgcctta tggcggagtt gaatacgggg agcgcattta
    18781 tggtctgacg ggccgatacc aagcctcggt gggcaagaac atggagctcg agatcgtcgg
    18841 cagttacggt catcggacca tcgagttttc cgacgacgag aactgggtgt acaactggta
    18901 tgggcagcgc gttcgccgcc gcttggttcc cggcgagatc gaggccaaag cgcgtgacac
    18961 ctcggtgtgg cagcacagcg gatttggaag ggcgatctac aagtggcagc tcagtccgcg
    19021 gcatgccttg ggtgcctcat acaccgccaa ttgggctcat cgcacgggcg acgagcgaat
    19081 ccaggtcgat cccgacgcgc gtgatccgct cacggccgcg cgcaagctgt tcacgctcgt
    19141 cggtgggctg gactaccaac tgagcttgtt cgacgatcgg ctatccaatg tcgtgttcgt
    19201 caaagattac ctttaccacg ccaacaccga ggagccgctg ccaggcggcg tgttccgcga
    19261 gcgaaccagt gacacccact cttggggcgt gggggattcg atgcgctttc gtttcacgcc
    19321 ttggctctat gcgaaaatgt cttacgagta cgcgacgcgc ttgccccggc cagacgaggt
    19381 gtttggtaat ggagtgctcg tgcaggccaa cctagaactc gagccagagg tcagtcacaa
    19441 cgcgaacgcg ggcccccggc tcgagttgcc aaagacacca attggtagcc tggtcgtcga
    19501 tctgaacgga tttctgcgcg atagcgatcg tttgatcgtt ctgctaggca atgaccgata
    19561 tttcacctat caaaatgttt atcatgccgg cggagtaggc ttggagagcg ccgtgaattg
    19621 ggtggtgcct cggcgttggc tgagcttgga tggcacgttg acgtggcaag accagcgtaa
    19681 tgtgtccaat actggcacgt tcaaatcctt cgagggcgac cggattccca accgaccgta
    19741 tctctttgcg agttgggcgg cgcgtcttcg ggtgcagggg cttcccggaa aacgagacac
    19801 tctggagccg tactactatg ggcgttacgt gcacggcttc tatagaggtt gggagagctc
    19861 aggggatcct cgcttcaagc aagtcgttgc gtcccaaatc acccatagtc tcggtatgac
    19921 ttggacggtt gaccgcacgg agcacaaggt cacctctact ctggaattcg agaatatcct
    19981 ggatacacgc gtgttcgaca actttggcgt ccaacgcccc ggacgaggtg tgtacttgcg
    20041 agtcactgcc gatatgtaaa gttcaggatt gtttcgtcct ggttatctca atggcgctgc
    20101 actagaaggt tcgaatggac gacctggccg agaacgttag aaccttcgtg agcttgatcg
    20161 agcgcgggga gaccttggcc gcgatcgagc gattctatgc aatggatgtg tgcgtattcg
    20221 agaaccggga gctcgcgcga gccgggcgcg agaactgttt gacttacgag cgcgagtcac
    20281 tcgggcgcat cactggattg ccccggttca aggtgcatcg attcgcagtg aatgaggccg
    20341 ccggtgtcgt attcttggag tacacggtgc gctttacggc cagctcaggg cgtccgatgc
    20401 ggctcgatga agttgccgtt cagacctggg aaggctcgct catcacacag gagcgcttct
    20461 attacgaggg gctggtcgac gagggggacg acgagccgaa gttggttgcg acctcgggct
    20521 gagcgcaacc acgaaatccc gagcggcggt gccccgagcc cgctgctcga gacggtttcg
    20581 cctatgcaac cgcatgcaag acagcatgcg ctctggattt cgttgtgatt tctgactacc
    20641 ggccccggtt tttgcggtcc tggcacgaga gagcttcggg gcgcccggcg gttggcccag
    20701 ggatgatctc gggctaatgt aggccgcaat ggatcggttg cgcggtgtcg gcctgggttt
    20761 taggcccgct ttggccagcg atctattgcg ccaacgcggg tgcgtcgatt tcgtggaggt
    20821 cgttgccgag gcatgcctgt tcaatggagc gcaacggcga gaggtgcggg ctctatcgga
    20881 attgtggcct gtcgttccgc acggcgtaaa gctctcgctc ggcagcgcga acggaatcga
    20941 tgtcgcgcga gtgcgggcgc tcgggcaact agcgcgtgag ctgaagtcca gcgttattac
    21001 ggagcacgtc gcgttcgtgc ggacactatc ccgagaaatc ggccatctca caccgctgcc
    21061 gcgtacagca acggctttgg gcgttctgga aaggaacttg ggcgtagcgc gcgcgcattt
    21121 gcccgatctg cccctcttgg tcgagaacgt agcttggacg gtacggtacc ctgagcgcgg
    21181 cgaaccacat ttccccgagc ccgagtttta ttcggagctg gttgagcgta ccggcgtagg
    21241 catgctgctc gatttgggca acctgtacgc gaatgccatc aacgcagggc tcgagcctga
    21301 atccgagttg aggcgttatc cattgcacgc agtgggtatg cttcatctgg ccggtggcat
    21361 tttcgaagat gggttttact tcgacgacca cgcacacgcc gttccggatc aggtgctagc
    21421 gctgttggaa acggcgctcg ccgcggtggg ggacgtgcca atcgtgatcg agcgggatgc
    21481 gctgtttcct ccgttcgctg agctgagtgg cgaggtctcc caagtgcggg cgatgctccg
    21541 gggccgggag cggcttccgg ttcacagctc tctctctgca cggcggccga ctcactctgc
    21601 gctccagcat cccgtgctcc agccgtccga gctccagcag ttcgaaagtc agcaggcgac
    21661 tttggccgag ctactgacca acttggagtt cccgaactcg agcgagttag ctggcttcga
    21721 ggcggccgag ttgtggcgca gtcgggccat tctgcagcgc aagcgcttgg aagaggccct
    21781 tcccctgctg gcgcgtctag gcgcctactg gcgcgatctc gagccctggg cggaacgagt
    21841 cattgcgcag tgtccgcgcg caccgaggct tgcggccgta accgacgcgc tgcagattgc
    21901 gtcccgggct caacgcgagt tcgactcgcc cgagatagct tttgcagcgc gagtcgacgc
    21961 tctggtgctc tcggcgcgct tcattggcga acccggtcaa gcgctgcgac caagacatcc
    22021 cccgttcgtg gcccacgtta gatcgccccg tggggcaaga ctctgggtga tcaaaggttt
    22081 tggatcttcg gcgcagactc gcatcgtaga aaggaacaca gcggtttgaa agatctcgac
    22141 tcgacaccac tacgtgagtt gccgcccgag ttgaagcggc agctgttggc ttttactgaa
    22201 cagctcgaaa acaagctcgg agcatcgcta gtcgctctgt tcgtattcgg aggagccgcg
    22261 cgtggagaat accatgtagg cagttcggat atcgactgcg ccatagtact cgactcggcc
    22321 acccctgaac tgctgatcgg gctcggaaac ttgcttcaac tcggacgctt ccagtcgcgc
    22381 ttcgagagca tcattctgac gctcgaggag gttcaacacg ccgccgatgc gtttccaatc
    22441 ttctattcgg acatgcgcag atgtcacgtt ttactcacgg ggcgggacgt actttcggat
    22501 ctgcagatct cggatcacca tctccgcttg cgggtggaac aagagctgcg ggattgccaa
    22561 attcatctgc ggcgcgcgat aacggatagc atgggcgagt cgcacgccct agccggcgcg
    22621 gtgatacgaa aggtaaagca aattcgggct ccgttgcgcg ccctggccca actcgaaggg
    22681 cgaagcgtcg ccgacaagat gggcgaagta ctggccgacg cggggcgccg attccaattg
    22741 gacgtgagcc ccctcagtga accggatcga gcgcccgtcg tctcggtcgg aacgttgatg
    22801 gagctcttga gtcgcagcat cgaggtggta gaccggatga gcggggaggc gtgaagtcgt
    22861 ggacttggga atcgatatct ttccgttcaa cttgccgccg ggcgggccgt tcctcgtatt
    22921 cttcgtttgg ttcgcgctga tcgtttgggg cggtgcgatg ctgagccggc acgctttgcg
    22981 tcgcccattc gagcgcttct tcctggagcg gtcgagtgct ctcggatcag gagtgcccct
    23041 ttacgccgcg aacgtcggca tgccggctcc agggtggtcg gccagcgcct ctggtccaag
    23101 cggcactccc ggcgcaggat tgctgaaagg gcgtattccg gtaggtgaac agctgtttgc
    23161 ggtggcgcag ctcaaggggg gaaaggcagg ggtaggcgac gtgatgatta cccaggcctt
    23221 ggggctcgga ttgctccgcc cgacggcgaa ccccgccgag ttagtctctt tgcagccgac
    23281 gacatcgctt cagccggcac cgctgatcac ccagcccgct cagtggtaca gtgttgattc
    23341 tgcggtgcgc gtcccgcccg gagtgatgag tcgtttcaga gctcgtttaa cgttgcactc
    23401 ttcgtcgccg ctcacgccag aaaagatcca cgagcaagcg gagcagttgg cggacgaact
    23461 cgaggacgga ctcgagcaac agctcatcgc ggacggactg cgtcgcagca aaagcgtgat
    23521 ctccatttgg cagaattggg tgctcgtccc cggaatcgtg gtgctaatca cggccgcggt
    23581 tcgcatcgtg ctgctagcga ttcacgaccg tccatttacg aacctactct ttgccgcggc
    23641 cgtcgccgca ttcatgttcg tggtcttctc gaaagttagc agcaaaaact gcttgcgagg
    23701 acaagaatac ttgtcttggt tggatgccgc gacttactcg attcagaccg aggtcaggaa
    23761 tggccgtcgc accgagccga acgaggtcag cttggtgctc gccgtgggtg gagccggcgc
    23821 tctcgtgggt gcggccgcct tcggactgct cactccgttg ttgtcaccgg cattccgtcc
    23881 catcgccagc gcgagctacg cgggcgacgg gagcggtagc tcgagctcgt gtagcagcag
    23941 tagcagctgc ggtggcggtg gtggttgcgg cggtggtggt ggctgcggag ggggcggcgg
    24001 ttgcagttga gacctgctag ctccgaaccg gcacgtttcg aagtgctgga cgcactcggg
    24061 gggtggtttc ggctggaagc aggtgaggcg accttggccg gctcgatccc gttgcgggcg
    24121 gcgcgggcct gctcgccgtt cctcgagggc aacgccatag gctatcgatt ggttaccgat
    24181 gcagtgttcc gggtgcagcg tcgactcgga ttgtggcgag tggaagtcag cgttcctgaa
    24241 gcggtgcgac gtgcgcagag ggccaacctt cctcagatag ccgtgacgat ggcgaacggc
    24301 ggcgcgacct ctggcgcgga cgcggcgcgg cgtggcttga tgacgcggct cgagcgtgag
    24361 ctggtgtgga tcgatggcgt gacctcttcg atccccaagc tgatcctgtt taccggcctg
    24421 ttcgtgcgcg ccttgcccga ctgcgaacta ttgctgagct cggccggcaa tcgccgcagt
    24481 gtgacgtacg gtgtcgaacc gggctgggtc agggcgaccg agctcgtgcc gctgctgatt
    24541 gagctcacct atcgaggctt gggggacatg acttgggaga ttacgggcga ggttgcctct
    24601 cttctgccct tgctacggcc catgtcgctc gagactcgga gtttgagcgc gcgctccaac
    24661 ttgggtgagg cgcacgcgcg cttcttcgat actaactact tcgcacagaa gaagcgcggc
    24721 cccacacgca aatacaagag tatgatcacc gaatctgggt cggctcgcgc agagcaagct
    24781 ccaagcacga ttggggaatg ggtggagatc gtctctcgac cgcaaactgc caacgtgcgc
    24841 gctgaaacga tcccggagag cgattgcggg gcggcccact tggtgcggaa cgcggtttca
    24901 ttcgatgcac gtttcgacgg ccaacatgtg gtcgttactt gcgatccggt cgagatgcgc
    24961 gagcacgcga atcggatcga agcggagtgg cgggctctgt atggcgagct gggtgacgcc
    25021 cgcggagcgc tcctctattt caccaagtac gccaccttgc accaagccgg cgaacccttc
    25081 ttcttcgtga aacctcccgc gctcgtgtcc acacctccgg gcacggcttg cctggtggag
    25141 gggttgtgcg gtccggaata cgacgtgctg cggggcgtga ttcgcactga cgttttccac
    25201 gccgtgccga ccgtgatccg cttgaactgg ccgggtaaga agattcgagt gcccaagggc
    25261 atgcccctcg cctgggtggt ccccttcgaa cgctcgagtc ctcacccaag ctatcggcgt
    25321 cgcgagctct gatgcgcgct atcgctggaa tagccgtgaa aacacttcct cgcgggtctc
    25381 gatgctcaca gtccgaattg caacactgag ttgccgaacg ttggaacgta ggctttgccg
    25441 ttcgccacga cgggaggggc aaacttcgag tagtagttgt agtcatacga gtagagcttc
    25501 ttgagctctt ggttgccgac gatcgtcgtc gcatcgaaca ccacgaatga gccctgtacg
    25561 acggcctgat tggcgtctcc gttcttgggg atcgttgccc aaagaaccgc gctgccggcg
    25621 gtcgtgccgt tcgaagacag tgcgagcatt ccgccgggca tgccacccgg cggcggcttt
    25681 cccgcggatg agtagaccgt gccctgattg cgaaaggcgt cgattcgctg cgtcgtaaag
    25741 ttgtagttgt agactttgac gcgctcgttc tctccccaca aatacacgtt cccgtgcgtg
    25801 gccatgcgca ggtacaccgg ggtgccgtgc agatgatgca tctttccgtc tggagttccg
    25861 tcgtgcagat tgcgatctag gtttacgatg ggccagttgg cgtccgcgag cgtcgatgtg
    25921 ggcagaccgg cagcgccgtt cggcgcattg ggtaggtaag tggcaacaaa aggcaaattg
    25981 aaatgagggg cccagttgtt gtgaccgaga tccgtgcggt tcagattgta cagaactcca
    26041 tctttgccac cgccgacgag gctctgaaag ccgggcacca gcagcacacc ggatgcgcct
    26101 aggtcttcgt cattgaacgc gcgcccgttg tcatcgaacg ccgtccaaaa gtcgctcagc
    26161 gcgagactgg gtttgccacc gttgcttcct gcggtacccg cggtataggc gagtttgacg
    26221 aagctctcgc cgtagttgct attgccgatc gcgccattcc cggtcgtgaa atagatgttg
    26281 ttgcccgcga tagccggggc tgagcccgcc atccagatcc cacccgctcc gccgttcggc
    26341 gtcacgcacc atactcccgg agtattcgtg aatcctgctt ggtggtgcag gcctcgggtg
    26401 tcgtacgcga agacgaaacc gttgcccggg cccgccggat tctcaccatt catcgagaaa
    26461 gccaagatga ccgccttgtt gcctgcagca tccgtgagca acccgagccc cgcgcgaagc
    26521 ttctgataag gcgtttcggt gccgacgttg aagtgtttgc cgccattgtc cgcgttgcca
    26581 cggatcaaga tgtcgggttg ttgattcgcg agcgtggtcg cgtccagcgt ccacaggtgg
    26641 tgctcacggt tcccgttgtc accggacccg ggtgcgcgtt ggaacgtgac gaggtacaga
    26701 ctgttggtag cgggatcgat caccggcgtc gacgcaattc cccacttgtg gtaaacgcac
    26761 cacatatcca tctgtccggg ctgcacacag ggatcgatgg cttcggctag gtgacgcgag
    26821 ttcaagagcg cgcccgtgtt cacgtcgaag cgatagaccg tgttgttcat gctgaacacg
    26881 tacagcgcgt tcccataaac tagaggttga gcttcgactt tctcatcgac gttgaaggtc
    26941 cggagcaaat gcagcgtggc gagattgttg ttcttgaaag tcgtttcggc cccattccag
    27001 ccacttctat tgttgtcgaa tgctcgagta gtgacgtcca ccgcactcgc tggagtgctg
    27061 atcgcgaacg aaatcagtat gaagcaagca ctcgcgagtg aggaacgttg catgcaagat
    27121 ctccatagct gggctgctaa ctagggccga gccgctaact agggccgagc cgcaagagca
    27181 ccggattcaa cgcgggctat tctcgtcaat accccgccga agtgcaagcg agagttcgac
    27241 tcgcacgaaa cgtggttacg agcctcgtct tttcgagcac caaggtatat gggtgaatga
    27301 ctatcgaatt ccgccatgat gctgggactt gcgcggcttc gggttaggcg tcttggtgat
    27361 ctgttgccgg atcttttctg gcaccgtgtc cgctagtcat atcttcgcga gactaggatc
    27421 ctgagtgcta aaccatctct gcctgtctgt gttttgttct actatgctcg gccatggcga
    27481 cccatcgcaa agcgtcagcg ggtttgtggc ttggagccgt agcgatccag atatgcgtcg
    27541 cctgcggcac ggctcggaat ccggagccga agtccgtggc agaagtcacg ccaaaactgc
    27601 aggagccagc ggctactccg cacggcaaaa gcggggcctg tggagatggg caatggtgcg
    27661 ttgcttcagg gattcccaaa gcggtcacgt tctcctcgat ctggggctcc agctccaacg
    27721 acatttggtt ggcgggcgaa gcgggcactc tcgtgcattg ggacggtgtg cagtggaccc
    27781 aggtcgaatc gggcacacag aattcgctac gcgcgttgac gggaacatcc gcgacggacg
    27841 tgtgggcggc cgggctcgag ggtaccctgc tgcactacga cggctcgacc tggtccggag
    27901 tgcagaggaa cggagagcct tggtctccca cgtctgggcc caatactcga ccgatttacg
    27961 cgctgtatgc cgctcaaccg cagcggcttt gggccgccgg ctctggcgcg cggtacttcg
    28021 acggtaccgc ctggagcgag cctcatcacg gttctcatct cccgattatg ggactctgga
    28081 gcagcggtga agcagatacg tgggcggtcg gttttcaagg cactatcacg cgctgggatg
    28141 gacaccactg ggagcgagtc gggcgggaca tgggccccaa ctacctcgcg gtttggggca
    28201 gcgcgcgcaa tgacgtttgg atcgtcggaa gcgcaggtgc ggcgctgcat tgggatggga
    28261 cgggctggag cgcgatcccg agcggcacca cgaacgactt acatgccgtg cgtggcttca
    28321 atgccaaaga cgtttgggcg gtcggggatc gaggtaccat cctgcattgg gaaggaagcg
    28381 cgtgggtggc ttcgccaagt ctctcggagc gcggtttgct cgcgctgtgg ggggcgagcg
    28441 cggcggacgt ttgggccgta ggtgagaatg ggatcgtctt gcgccgccag aactaagcaa
    28501 gtgttcgtac cgtctcgcga cgaagctcgt ttggggccgc tccgtaggga aaacacgaaa
    28561 accactcggt gactcgctgg gcggggggcg ccggttctcg agaggccgct gcccggtttc
    28621 gacgctgctt tttgtctcat tccggcagct agctgatccg gtacagttcg aggatgggct
    28681 ttagtgtcgg ctcgtcttcg caggaaatgg agcgggccag agggttagcc cgggtcgctc
    28741 gggccttggc ggagctggag gatcaggaga cagcgttgaa ggaagtctgc gagcaggtgc
    28801 gtcacgtgct cggcacgacc gcatcagcca tcaacctact cgatgaaggc ggggagttct
    28861 ttcattggtc tgcgcagtgt ggccacccgg agacatttcc gctcgagcct attccttggc
    28921 agctattcga cgcctttgcg gcaggcttcg agccgatcct cagcgagacc cgacccgaag
    28981 tggtgcgcgc cgacccgcgt ctcgcgatgt tctccgtgca cgggttgcag gcctttgcca
    29041 cgattcccgt gcgtcgctcg ggacgcctac ttgggttgat taccgcggcg tcatacggca
    29101 ccaccagagc accgttcgat gcgtccgagg cggtttggct gcagaccgtt ggggacatgg
    29161 tcgtccaaga ggtgctgcgg cagcgtgcat tgttcgccgc gcgcgagagc gaggcgcgct
    29221 acaagcgcat cgtctcgacc attcgggatg gcgtgtgggc catcgatgcc gatcaacgta
    29281 cggagtacgt gaacccgcaa atggcggaca tgctgggcta tcgagtagag gagttgatcg
    29341 gaaaccgggc tcctgaattg aacgagtcct ggccgggcgc cggctacaag cggtgggcgg
    29401 atcgtcagca aggccgggcc gagcgatacg aggtccactt ccggcgcaag gacggaaaag
    29461 aggtgtatgc gtccgtttcc gccgcgccgc tgttcgacaa agaaggcaat ttttcaggct
    29521 ctttgagcgt ggttacggac atcaccgagc aacgtaagct ggagttgaaa ctgcaacaga
    29581 cgcagaagtt ggagagcctg ggtattctcg cggggggcat cgctcatgat ttcaacaatc
    29641 tgttggtggc gatgttgggc aatgcttccc tgggcttgga aatagtccgc gatgactcgg
    29701 agttggtccc catcttcata gatatccgct cggcggcgca aagggccgcg gagctgaccc
    29761 ggcagatgct ggcctactcg ggccgaggtc gcttcgtcgt gcgcgatttg gagctgaatg
    29821 cgttgatccg ggagaccgcc aatctgatcg gaaccgtcat gcccaagaac gcagagctgc
    29881 agttggtgct ctcgcccggg ctgcc
//
LOCUS       DASTKU010000637         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1014292, whole genome shotgun sequence.
ACCESSION   DASTKU010000637 DASTKU010000000
VERSION     DASTKU010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014292"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(306..881)
                     /locus_tag="VFQ61_22275"
     CDS             complement(306..881)
                     /locus_tag="VFQ61_22275"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9957248.1"
                     /translation="MGYQNFYVITGASGAGKSTLIAALADLGYATIPEAALSVLREQR
                     KANSEVLPSVDRAAFMQAVLARSIGDYETALSLAKPVFFDRGIPEWLRFLDSGGNEHR
                     QVAARCRYASTVFLAEPWPEIYVRDHERQASFDRAARSYEATVSAYIEAGYDICAIPK
                     VSIQERVAFMLAQTGLAPDNSFGPVPPRGAA"
     gene            complement(1031..1438)
                     /locus_tag="VFQ61_22280"
     CDS             complement(1031..1438)
                     /locus_tag="VFQ61_22280"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9957249.1"
                     /translation="MNPEVDALKQFFAAINRNDMQAMTRDFDPQIVRVEPEGFPTAGT
                     YRGIAEVQEHVRAGRGTWAEGSCEPEKFLVNGEKVVVYLHAWVRLKGATEWTGGRFAD
                     GFVFRNGRITEYRSFGERAQALEWAGIQDQEPS"
     gene            complement(1732..>2073)
                     /locus_tag="VFQ61_22285"
     CDS             complement(1732..>2073)
                     /locus_tag="VFQ61_22285"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9957250.1"
                     /translation="RSIEVLPAVAGHVATMMRGTNPEGAAAALRGRADRPSYEDTLAS
                     LAMPALVVVGSEDAFTTRRDAERMRDLLTGSELVWLEGVGQMPNLESAAAFNAALTRF
                     LERLEIAGATA"
BASE COUNT          385 a          696 c          628 g          364 t
ORIGIN      
        1 acaccggcat acagcgtcgc cacactgcca accagcacga cgatggcggt gatcagccag
       61 agggcgaaga acccgcggag gaggcgacga ggcggcatag cacatactcc gaccgaagtt
      121 gatctgcttg gcggcccaac gcagagctac gcgacgaatg accgctcggc aagcctgaaa
      181 tgctcggcgc tcgccagtca ccttaccacg atgagctcca ccgtcctcgc gctcgtccgc
      241 tcgtgcagag ctctccagcg agaacctctt catggcacgg tctccgcgtt cgttcaacgc
      301 ctgagtcaag ccgcgccgcg aggcggcacc ggcccgaacg aattgtcagg cgccagcccc
      361 gtttgggcaa gcatgaacgc tacacgctct tgaatcgaca ctttgggaat ggcacaaata
      421 tcgtaacccg cttcgatata cgctgaaacc gttgcctcat aggaccgagc cgctcggtca
      481 aagcttgctt gccgctcgtg gtcgcgcacg tagatctccg gccaaggctc cgctagaaat
      541 acggtactgg catagcggca acgcgcagct acctggcggt gctcgttccc gcccgagtcc
      601 aggaaccgca gccactcggg gatgccacga tcgaagaata cgggttttgc cagggataga
      661 gccgtctcgt agtctccgat gctgcgagcg agaactgcct gcatgaacgc cgcacgatca
      721 accgacggaa gtacttcgct gttggctttc cgttgctctc gcaagacgga gagggctgct
      781 tccggaatgg ttgcatagcc aagatcagcc agcgcagcta tgagcgttga cttgccagct
      841 ccggacgcgc cggtgatcac gtagaagttt tggtagccca tgatgctggt tctggtgcct
      901 tccgcccgag ttccgctgcg gggcgtacct taccgaacct accgcgacgg ccggacccgg
      961 gtgcccccca ccagctgcaa cgcctcgaca ggccgcgctg gcagcgtcgc tgacgctact
     1021 tgcgtgcgac tcaactcggc tcctggtcct ggatgccggc ccactcgagc gcttgcgctc
     1081 gttcaccgaa ggatcggtac tcggtgatcc tgccgttgcg gaacacgaag ccatcggcga
     1141 accgaccgcc agtccactcg gttgcgcctt tcaggcgaac ccacgcgtgg agatagacga
     1201 cgaccttctc gccgttgacc aggaacttct cgggctcgca gctcccttcg gcccacgttc
     1261 cacgcccggc cctgacatgc tcttgcactt ccgcgatgcc gcgataggtg ccagcggtcg
     1321 ggaacccctc gggttcaacg cgtacgatct gaggatcgaa gtccctggtc atggcctgca
     1381 tgtcgttgcg gtttatcgca gcgaagaact gcttcagcgc gtcgacttcg gggttcatgc
     1441 gctcgcctcg aggtggtgga tgttgcgcgc cctgacgccc cagcttggct gcgagcacga
     1501 agcacacggg ggcgaggcgc gacgatggtc gatcgtagct agtctcgcca cccaatcacg
     1561 tggcagcccc ggagcgcttg tccgatgcaa cgactcgcca ggcaccactg cgccatgatc
     1621 tccgatggtc acggcgcgat gaaggcgcgg tgttccctac cacaagagcc gaaaatagta
     1681 ggttacgcgc gctcccgtgt acagcataac cgggcaccga ccagataggg ctcatgccgt
     1741 agcgccggcg atttccagcc gctcgaggaa tcgggtgagg gccgcattga aggccgccgc
     1801 gctctcgaga ttcggcattt gcccaacgcc ctccagccag accaactccg accccgtcag
     1861 gagatcgcgc atccgctcgg cgtcccggcg cgtggtgaag gcgtcttcgc tcccgaccac
     1921 gacgagcgcc ggcatggcca gactcgcgag ggtgtcctcg tatgacgggc gatcggcacg
     1981 cccacggagc gcggcggctg ccccttcggg attcgtgcca cgcatcatcg tggcgacgtg
     2041 gccggcgacc gcaggcagca cctcgatgct acg
//
LOCUS       DASTKU010000638        19174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_568779, whole genome shotgun sequence.
ACCESSION   DASTKU010000638 DASTKU010000000
VERSION     DASTKU010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 19174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19174
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568779"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1326)
                     /gene="eno"
                     /locus_tag="VFQ61_22290"
     CDS             complement(55..1326)
                     /gene="eno"
                     /locus_tag="VFQ61_22290"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET9957251.1"
                     /translation="MPEILQVSAREILDSRGNPTVEAEVHTSSGFGRAAVPSGASTGE
                     HEAIELRDGDKKRYLGKGVKQAVENVNEKLGPAIVGMSALDQAAVDHRLIELDGTANK
                     SKLGANAILAVSMAVARAAADTVGLPLWRYLGGVQARVLPTPLMNIVNGGAHADNGLE
                     IQEFMVVPLGFNSFGKALRAGTEVFHNLKSILKKEGLATSVGDEGGFAPRLGSNEDAL
                     KRIIQAIEAAGYTPGEDVALALDCAASEFYDNGTYTFDKRKVSPADLASIYVDLSEKY
                     PIVSIEDGFAEDDWDGWKLITDKLGNKLQLVGDDLFVTNKERLAKGIEKGIANSILIK
                     LNQIGSLTETLDTIRLASTSGYTSIISHRSGETEDAFIADLAVATNAGQIKTGSLSRS
                     DRIAKYNQLLRIGDELGDEQVFSGGTPFKRR"
     gene            complement(1499..2530)
                     /locus_tag="VFQ61_22295"
     CDS             complement(1499..2530)
                     /locus_tag="VFQ61_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET9957252.1"
                     /translation="MTEAAHSATLEVENTTVLMSLLGPQSALLTEVAREAGADVSLRG
                     NTIYISGEEAHVKLAYRFLSDAAELVGRGFTIGPSDVPGSLRGLRADPNRTLVDLVDE
                     TILITPRKRPIVPKSLGQRRYVEAIRTHDLTFGIGPAGTGKTYLAMALAASWLIQKRV
                     KRIVLARPAVEAGEKLGFLPGDLAEKVNPYLRPLYDALYDMLEYERVEQLKEREQVEV
                     APLAFMRGRTLNDAFVILDEAQNATSEQMRMFLTRFGFGSKVVVTGDITQTDLPQGSR
                     SGLREARDLLENVPGIAFCSFTDEDVVRHPLVQKIIVAYEKRDEAMKNERASERPRRE
                     DRGPRFDPR"
     gene            complement(2799..3794)
                     /locus_tag="VFQ61_22300"
     CDS             complement(2799..3794)
                     /locus_tag="VFQ61_22300"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HET9957253.1"
                     /translation="MKGGGGGAAAGGGGPKVIIIKKKGKGHGGHHGGAWKVAYADFVT
                     AMMALFMVMWLLSQTDQETRQKLSEYFRTGMFSGAPSVLTGGSGIQERAFFDVVGRSA
                     MQPESAAFERVALAVRAHLQRMIDEQPDLEDLGKNVKVSVTADGLLITIIDGGDDLMF
                     DLSSSELKPRLVALLERLAPILAELDNPLQIHGHTDGRPFPKGSSRTNWALSFERADR
                     ARAVLERSGLRIKQIQGVFAHGDTNLLNTHNSYAPENRRLAILAVRKNPEADPKAQAQ
                     PGVQGQPGAKGQPGAKGQPGARRTSDAAAERDAEGPRGSTSRPKTKEQEAEDGEE"
     gene            complement(3794..4654)
                     /gene="motA"
                     /locus_tag="VFQ61_22305"
     CDS             complement(3794..4654)
                     /gene="motA"
                     /locus_tag="VFQ61_22305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HET9957254.1"
                     /translation="MSTPVGLVIVLVCVIGGFLMAGGPLPVLWQPNELIVIGGAAMGS
                     LVVSAPGRAMKRVTSAFKKGFGNHSPVKQDYLDLLKMLYQILALIRREGVLALESHTT
                     DRASSSIFTAYPSVSKRHHAMDFLVDGLKQLVDGCSAEELAMLFDAELETHHEEEHQP
                     INLIRTTGDALPGMGIVAAVLGIVITMAHLDGGPEEIGHHVAAALVGTFLGILLCYGV
                     VGPIATSIELQSAAEGRYMLCIKEAVVAAARGVNPAIAIEFARRSIFSDERPTGPELE
                     KAFAELKGKS"
     gene            complement(4737..5219)
                     /locus_tag="VFQ61_22310"
     CDS             complement(4737..5219)
                     /locus_tag="VFQ61_22310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957255.1"
                     /translation="MRSSSAPAGHRFPGGMSLTDYRSRIRFVRWLLPASLWVVVADAK
                     PEEASFRVRTHNHEFHRVKVEARECELAYRLYFTAPELEPSKAGKPREFRFQARLRFQ
                     DGKSFLSPVFKGLAAGERMYENVYDTSSEGCWAKSLSKVAGVDVEACRGRGCTPAEPH
                     "
     gene            complement(5224..6750)
                     /locus_tag="VFQ61_22315"
     CDS             complement(5224..6750)
                     /locus_tag="VFQ61_22315"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HET9957256.1"
                     /translation="MKTKLLRSIQAATRALNCSAIPSWISRGSGPVAGLICFSVTSAA
                     LAQAAAKAPLAAAQSAVSDASQRKNVSLTVYNQDLGLVREVRQLSGLPIGRMALEYRD
                     VASTIQPETVHIRALSGPGTLSVLEQNYRYDLLTPQTLLEKYVGKDVRVYRYHQNSGR
                     EDVTDARLLSVAGNPVLSINREITFDYGGRFAFPTLPENLIEKPTLFWLLDGKSAKQE
                     VEVTYLAGGLNWSADYVAVLDDNEQRADLRGWVTLVNQSGSSYKDADLKLVAGNVNRV
                     APARDMVAMESRKVMAAPRAAQFAEEGLLEYHLYSLGRKTDVLNNEQKQVALVEAKGI
                     GIEKKFTFSGETYWFRQPDATPRKNQKVGVYLEFENNQKNQLGMPLPKGVVRVYKADS
                     TGSQQFVGEDSIDHTPRDERVRIKLGEAFDVVADRKQVRYTVLGECSSESTWEIEFRN
                     HKDTAITIEDLEPASGDYNVVSSSQPAERKDANTFKFDVKVPARGKTRLNYVVRVRWC
                     "
     gene            complement(6762..8252)
                     /locus_tag="VFQ61_22320"
     CDS             complement(6762..8252)
                     /locus_tag="VFQ61_22320"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HET9957257.1"
                     /translation="MRLPSNGWIRSRRAGLMLASLAAAGLLGCSSPKPAAAPRAAHSA
                     LSTSRDREQISVTVYNQDLGLIRERRSIALGSGLVALTFEDVAQKIQPESVHVRSVSH
                     PGQAEVLEQNYRYDLLSPENLLKKYVGKSVRVYRYNEQLGRDEVKTAQVLAAEKGVVL
                     KVDGQITSGTDLGRLAFPEIPNNLVSQPTLEWLLDSGTPRQQLEVSYLSGGLNWHADY
                     VLSLDGDGARGELASWVTLTNESGVGFENADLKLVAGQVNRLAPPREEDADDYKENAT
                     LADTQGLREEALSEYHLYRLDRPTTLRHKERKQVSLFGTARVGVQKKLVFTANMPRLL
                     IASTMPAERKPLEHARVVLELQNSQQNGLGMALPQGILRAYTRDAAGAAQFIGEDEIE
                     HTPRDETLSVSVGEAFDVVLERVALKRSQVSRCTLETDWRLDIKNHKDSPETVELREN
                     TGGTDYELVSTSLPPKRQTRDQFSYVLSVPARGATRVEYRIRVRSC"
     gene            8329..9420
                     /gene="proB"
                     /locus_tag="VFQ61_22325"
     CDS             8329..9420
                     /gene="proB"
                     /locus_tag="VFQ61_22325"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET9957258.1"
                     /translation="MSARSEFIKARRIVVKIGSRSLIHDGELIRRLSQQVAELAGQGR
                     SVIVVSSGAIALGCRRLGYRSKPKELARLQAAAATGQSVLMRRYDEAFGELGLTAAQV
                     LLTHADLADRERLNNARETFAALLEASAIPVVNENDTVSTEEIRFGDNDQLASMVVPL
                     VSADALVLLTDVEGVLDGSGRRIPIMTGAEKLELIGERRERVGTGGIQSKVDAARKAA
                     QSGASVVIAPATRPDVLRALFAGEDIGTLFPSRQNPLRARKHWIAFTLRTRGTLLLDE
                     GATRAVRAGKSSLLPIGVMGVRGAFNPGDAVRLIGPDGAEIGRGLTRLGALDAARSAG
                     RKGTELDLLFGSGGADLVVVHRDDLVVTD"
     gene            9716..10582
                     /locus_tag="VFQ61_22330"
     CDS             9716..10582
                     /locus_tag="VFQ61_22330"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9957259.1"
                     /translation="MALSIAGCALTVACARGPAAPALVPKPASALGEERGPAVSEDGV
                     PQALTAPEASDWVSEDDFTVRVITDAITCTGALIAPDLVLTAHHCVAARSSQGDIVER
                     DVTSSEVTVELGGDRFPLGEAGVRSVVSPPCGYSGGAGDLAILVLSRSLPGLPHRAVE
                     LERMPRAGDSVIPLGFGRCAGTNDGIYRKRRAATPLHVVQEGRMLLDAAICPGDSGGP
                     ALSSSTGRVVGVVSRSVMDADEATLGFTELSRLDGFRELFAAAAQVARGTSRAELPPI
                     ECPVMGGRTSQR"
     gene            10693..11337
                     /gene="lepB"
                     /locus_tag="VFQ61_22335"
     CDS             10693..11337
                     /gene="lepB"
                     /locus_tag="VFQ61_22335"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9957260.1"
                     /translation="MSKLLRFLVWMGIAAGITVGISRAVALRWWKVPSGDPYLEASVA
                     PTLRGGDWVLLWRLSAPRLGDLVLCPEPNAPGREVLARIAGVQGDRVELDGGMLRING
                     RKAAANHPCPEDHFTTIDPETRAATPQSCVIEELGSRNHPRGEVTSQLRAADHKSFEV
                     PAGQVVLISDNRQFPYDSRDFGLVDRASCRESVFFRLVSAAGFSDVATRLTWIP"
     gene            11410..12804
                     /locus_tag="VFQ61_22340"
     CDS             11410..12804
                     /locus_tag="VFQ61_22340"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HET9957261.1"
                     /translation="MNWRLVLGLCVLASSGSLYAAGAVWGARIVEQRLRPLNVWVDGW
                     VLTPGANEGALVEQLARRTAERRAFLKDGGELYGISFGELGIELDRSALLERLRAARP
                     DNGWRARWSRIWRAPGPEPEVRTRLACDEERAARVITSYASVLARAPVNARLDLAAHR
                     RIEDQPGRELDVPATLNELRQRAFDGTPVFDARFHELPARVRSESLPPVDVSRVLASF
                     ETDFSKKPMSRRPNIEAAARYLEGFVLQPGAVLSFNKVVGPRTFERGFREAPVIVADE
                     LESDLGGGVCQVATAVFAASALGGLEILERRSHSRPSGYAPLGLDAAVVYGKVDLRVR
                     NPYPVPVLLHTSFPTPKSLRVELLGLQAPQQVEHSFRVRERKPYHRKVVIKTDVPPDA
                     IEQKQKGNAGYEVVSTLRSVASDGSVRRREYPSRYYPVPEVFWIGTAVDPNSLPALAE
                     DETHVEIERAPQAL"
     gene            complement(12810..13094)
                     /locus_tag="VFQ61_22345"
     CDS             complement(12810..13094)
                     /locus_tag="VFQ61_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative motility protein"
                     /protein_id="HET9957262.1"
                     /translation="MGGDSNRIWETDSKKEFKVRHATPFSGGEMGSNGISASSALTAA
                     QMRAEFDVKVARKALDNVEQQGQNALKLIDAASAPSNVANGVGQRLNVVA"
     gene            13175..13474
                     /locus_tag="VFQ61_22350"
     CDS             13175..13474
                     /locus_tag="VFQ61_22350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957263.1"
                     /translation="MHQSCEVCAQFLPARGVSKIPDPTAWVPFVQADPTDPRTVALCR
                     THLHLVWAAGAKTWSELVELFRENEGRRSWLPRRQVTRTAHPGRRATDRAKDPTG"
     gene            13705..15429
                     /gene="leuA"
                     /locus_tag="VFQ61_22355"
     CDS             13705..15429
                     /gene="leuA"
                     /locus_tag="VFQ61_22355"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET9957264.1"
                     /translation="MAYNPRRYRAPETIRLSDRTWPERTLERAPTWCSVDLRDGNQAL
                     AEPMGMERKQRMLAALLEVGFREIELGFPSASQTDFDFIRWVIEENVLPEGVTPQVIT
                     QARPALIERTFESIRGAKRAIFHLYNSTSEVQRRVVFGLSEAEVIALAVRATEQIRAL
                     AAQMPETEIIFQYSPESFTGTELAFAKAICDAVAEAWGATPERKMIVNLPSTVENTTP
                     NVFADRIEWMSRNLARRDSIILSVHTHNDRGSGVASAELAQLAGAERVEGTVFGNGER
                     SGNVDIVTLALNLYSHGIEPQLRFDDLPALVKVIEYCTRLPVHPRHPYAGELVFTAFS
                     GSHQDAIHKGMKRVGEGPDDIWEVPYLPIDPMDVGRRYESVIRINSQSGKSGLAHVLE
                     RDAGYRVPRPVAIEFGRVAQAVTDGRGEELSSEGVVALFERTYLSVSGPFELRTVAVE
                     RRSAGRDCAVTAELRIDGVSRTVTGIGSGPIEAFVSALSTCVGQTLEVADYVEHARGR
                     GADAEAVAFASVRGAADAGEPLYGVGRHKDVVLASLHAIIAACNRLHAASRFAQRGPT
                     VVAVDIAP"
     gene            complement(15376..16434)
                     /locus_tag="VFQ61_22360"
     CDS             complement(15376..16434)
                     /locus_tag="VFQ61_22360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HET9957265.1"
                     /translation="MKTRNAGGLLALLSVAACAGWASACDGPTAPELFNESAGGVRGG
                     ATSSSAGGVSSPSGGSSTDSIGSGTSPSSGGTSLSESGGTGNGGASSGGLSSSETSPN
                     GDGGKTGNSGGGSGAPHSGGAIQSGGRADPGGAPTSGGQPQGSGGLISSGGAGGSAGQ
                     DMGGSGGDDCSTPNALRGEERCDGLDNDCDGAIDEGELCPTSCVGLGSSEHSWMLCTQ
                     ELDFDAAAKLCATAGMHLAWLKSDAANKQAFELIMKHDPELRRVWLGASDSAKEGQWQ
                     WVTERAQPGEAFWTGATPAAGGSPVGGAFSGWGAERPGDRSGGDDCAVMVISRAVVEP
                     GLWSDVDCDDGWAALCEP"
     gene            17045..17587
                     /locus_tag="VFQ61_22365"
     CDS             17045..17587
                     /locus_tag="VFQ61_22365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HET9957266.1"
                     /translation="MVTTTSNEHADRSFFVANGVVSALALSLIAFILLRDGGGAQDTR
                     LAFVPALNAVFNALSATCLVLGYVAIRRGARRSHQRFMISAFALSSLFLVGYLAYHSV
                     HGDTKFTGTGPIRAVYFFVLISHIVLSIGVVPMALTSFYLAFKQQFARHRRLNRVFLP
                     IWLYVSVTGVLIFLLQRLAA"
     gene            18829..>19174
                     /locus_tag="VFQ61_22370"
     CDS             18829..>19174
                     /locus_tag="VFQ61_22370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957267.1"
                     /translation="MEVSASSAAPAQALRVAFVVQRYGLEVNGGAELHCRELAERLAR
                     RPDVAEVRVLTTCAKRHVDWRNDYSPGTERLGGVVVERFVTDRPRAKLLSESLQWLLA
                     HLPHPRGLEQSWL"
BASE COUNT         3207 a         6072 c         6128 g         3767 t
ORIGIN      
        1 ctcgctcgtt agcggccatg ggtgcgccgc ccgattcagg ggagcgctcg agtctcagcg
       61 ccgcttgaac ggcgtaccgc cggagaacac ctgctcgtcc ccgagctcgt ctccaatgcg
      121 cagcaattgg ttgtacttgg cgatacggtc gctgcgactc aagctgccgg tcttgatttg
      181 gcccgcgttg gtggccacgg ccagatcggc gatgaacgcg tcttcggtct cgccggagcg
      241 gtgcgagatg atcgacgtgt aaccggaggt cgaagcgagg cggatggtgt cgagcgtctc
      301 cgtcagcgat ccaatttgat tcagcttgat gaggatgctg ttcgcgatgc ccttttcgat
      361 gcccttcgcg aggcgctctt tgttggtgac gaacaggtcg tcgcccacga gctggagctt
      421 gttaccgagc ttgtccgtaa tgagcttcca gccatcccaa tcgtcctcgg caaaaccgtc
      481 ctcgatgctg acgatggggt acttctccga tagatcgacg tagatactcg ccagatcggc
      541 agggctcacc ttgcgcttat cgaaggtgta cgtgccgttg tcgtagaatt cgctcgcggc
      601 acaatcgagc gccaacgcca cgtcttcgcc aggggtgtag ccagccgctt cgatggcctg
      661 gatgatccgc ttgagtgcgt cctcgttgga gcccaagcgc ggcgcaaaac cgccctcgtc
      721 acccacggac gtcgccaggc cttccttctt caggattgac ttcaagttgt ggaacacctc
      781 ggtgccggcg cgcagcgctt tgccgaacga gttgaaaccc agcggcacca ccatgaactc
      841 ttgaatttcg aggccgttgt cggcgtgcgc cccgccattc acgatgttca tcaagggcgt
      901 cggcaacacg cgagcttgca cgccgccgag gtagcgccag agcgggagac cgacggtatc
      961 ggccgcggcg cgggccacgg ccatcgagac cgccaagatc gcgttggcgc cgagtttgga
     1021 tttattggcc gtgccatcca gctcgatcag tcgatgatcg acggcggctt gatccagagc
     1081 tgacatacca acgatggctg ggccgagctt ctcgttgacg ttctctacgg cttgtttgac
     1141 gcctttgccg aggtaccgct tcttgtctcc gtcgcgcaac tcgatagctt cgtgttcacc
     1201 cgtagaggca ccgctcggca ccgcggcgcg gccgaagccg ctgctcgtgt gcacctcggc
     1261 ttctaccgtc gggtttccac gtgaatccag gatctctcgg gcactgactt gaagaatctc
     1321 cggcatgcgc gcccgattaa catggaatca gatggcgggc gagtcgccgc gcgcatcgtc
     1381 acatgttcga ttccatgagt gtcagcgccg tgcgatccaa cgccgtgtga ccgaattcgc
     1441 cggacccttc gattgccgag agtcgcacct gagagtgcga tcagcggcat cgcgacgctc
     1501 agcgcggatc gaaccgagga ccgcgatctt ctcggcgcgg ccgctcactc gcccgctcgt
     1561 tcttcatggc ttcgtcacgc ttctcgtaag cgacgatgat cttctgcacc aacggatggc
     1621 gcactacgtc ctcgtccgtg aaggaacaga acgcgattcc cggtacattc tccagcagat
     1681 cccgagcttc gcgcaggccg ctgcgcgagc cctggggaag atcagtctgc gtgatgtcac
     1741 ctgtcacgac caccttcgat ccgaagccga agcgcgtcag gaacatgcgc atctgttcac
     1801 tcgtcgcgtt ctgcgcctcg tcgaggatca cgaacgcatc attgagcgta cggccacgca
     1861 tgaacgcgag cggcgccacc tccacttgtt cgcgttcctt gagctgctcc acgcgctcgt
     1921 attcgagcat gtcgtagagc gcgtcataga gcggccgcag atatggattc accttttccg
     1981 cgaggtctcc cggcaagaag cccagcttct ctcccgcctc caccgcgggc cgagccagca
     2041 cgatacgttt gacgcgcttc tggatcaacc acgaggcggc caatgccatg gccaagtagg
     2101 tcttaccggt gcctgccgga ccaatgccga aggtgaggtc gtgggtgcga atggcctcga
     2161 cgtaacgccg ctgccccaag gacttcggaa ctatgggtcg cttacgcggc gtaatcagga
     2221 tggtctcatc caccaagtcc accagcgtgc gattgggatc ggcgcgcagc ccgcgcagtg
     2281 atccaggcac gtcgctcggc ccgatcgtga agccgcggcc cacgagctcc gcggcatcgc
     2341 tcaggaaccg atacgcgagc ttcacgtgcg cctcttcacc cgagatatag atcgtattgc
     2401 ctcgcaggct gacgtcggct cctgcctcac gcgccacttc cgtgagcagc gcggattggg
     2461 gcccgagcaa gctcatcagt accgtcgtgt tttcgacctc gagcgttgcg gagtgcgcgg
     2521 cttcggtcat caaaccgcgc tcccgaaggc gagcgcgccg taactcgagg gctgaacgga
     2581 ggcgtagggg cgaaacgatg acatgagcac accgagcata gcgggtgatc gcctgcggat
     2641 cgcgcaaatt gtcgacactt agcccgatct ttcgtcggac cgacgacgcg ctggctattc
     2701 agcccaacga ctaagccgct gaagctcgag aagcgggcat ttcgccccaa gcgcgcgcgg
     2761 ctgggcccga aactcgcctg cgctggcggc ccgcgctcct actcctcgcc gtcttccgcc
     2821 tcttgctctt tcgttttggg ccgagaggtg ctgccgcggg gcccctcggc gtctctctcg
     2881 gcagctgcgt cggacgtcct ccgcgctccg ggttggccct ttgcacccgg ttggcccttt
     2941 gcacccggtt gaccctgtac gccgggctga gcctgcgctt tcgggtctgc ctcaggattc
     3001 ttgcgcacgg ccaagatggc caggcggcgg ttctccgggg catacgagtt gtgcgtattg
     3061 agcaggttgg tgtcaccgtg cgcgaatacg ccttgaattt gcttgatgcg cagccccgag
     3121 cgctcgagca ccgcgcgggc tcgatcggcg cgctcgaatg agagcgccca attggtccga
     3181 ctagagccct tcggaaaagg acgtccatcg gtgtggccat ggatttggag ggggttgtcg
     3241 agctcggcca agatcggtgc caggcgctcc aacaacgcca ccaggcgcgg cttgagctcg
     3301 gacgacgaga gatcgaacat caaatcgtca cctccatcga tgatggtgat gagcaggccg
     3361 tccgcggtca ccgaaacctt gacgttcttg ccgaggtctt cgaggtccgg ttgctcgtcg
     3421 atcatgcgct gcaggtgggc ccgcaccgcc agggcaaccc gttcgaaggc agccgactcg
     3481 ggctgcatgg cagagcgacc gaccacgtcg aagaaggcgc gttcctgaat gccggagccg
     3541 ccggtcagga cgctcggcgc tccagaaaac atgcccgttc ggaaatactc ggacaacttc
     3601 tgtcgcgtct cctggtcggt ctgcgacaag agccacatca ccatgaacag agccatcatc
     3661 gccgtgacga agtcggcgta ggctactttc cacgccccgc cgtgatgacc gccgtggccc
     3721 ttgcccttct tcttgatgat gatgactttg gggccgcctc cgcccgcggc cgctccacca
     3781 cctccgccct tcatcaggat ttgcctttca gttcggcgaa ggccttctcg agctcagggc
     3841 ccgtcgggcg ctcgtcggag aagatcgagc ggcgcgcgaa ctcgatggca atggcggggt
     3901 ttacgccacg ggccgcggct acgacggcct ctttgatgca gagcatgtag cgaccctcgg
     3961 ccgcggattg cagctcgatg ctggtcgcga tcgggccgac gacgccgtag caaagcaaga
     4021 taccaaggaa ggttccgacg agcgcggcgg ctacgtggtg accgatttct tcggggccgc
     4081 cgtcgagatg cgccatcgtg atgacgatgc cgagcaccgc cgccacgatg cccattccgg
     4141 gcagggcgtc gcccgtcgta cgaatcagat tgatcggctg atgctcttct tcgtggtggg
     4201 tctcgagctc ggcgtcgaac agcatggcga gctcttccgc cgaacagcca tccaccagct
     4261 gcttcaaacc atccacgagg aaatccatgg cgtgatggcg cttggagacc gaagggtaag
     4321 ccgtgaagat gctagacgaa gctcgatcgg tggtgtgcga ctcgagcgcc agcacgcctt
     4381 cccggcgaat cagggccagg atctgataga gcatcttcag caggtccaag tagtcctgct
     4441 tgactggcga gtgattgccg aagcccttct tgaaggcgct ggtgacgcgc ttcatcgcgc
     4501 ggccgggcgc ggacaccacg agcgaaccca tggcggcacc gccaatcacg atgagttcgt
     4561 tcggctgcca aagcacgggc agcgggccgc cggccatgag gaacccgccg atgacgcaga
     4621 caagaacgat gactagcccg accggagtcg acacaacctc tctaggcggc cacccggggg
     4681 agttccttta gactcttacg tcccagcgcg agacgtgaat agccaagaac ctgcgctcaa
     4741 tgtggttcgg caggcgtgca gccgcggccg cgacatgcct ccacgtctac acccgcgacc
     4801 ttcgataggg acttcgccca acaaccctcg ctgctggtgt cataaacgtt ttcgtacatg
     4861 cgctcaccgg ccgcgagccc tttgaaaacg ggcgagagga agctcttgcc gtcttggaag
     4921 cggagccgcg cctgaaagcg gaactcccga ggctttccgg ctttcgaggg ttcgagctct
     4981 ggagccgtga agtacaggcg ataagcgagc tcacactcgc gcgcctctac cttcacccgg
     5041 tgaaactcgt ggttgtgcgt gcgcacgcgg aacgaagcct cctccggttt cgcgtccgcg
     5101 accacgaccc acaagctcgc tggtaggagc caacgaacga atcgaatcct cgagcggtaa
     5161 tcggtcaagc tcatgcctcc gggaaagcga tgcccggccg gtgccgagct cgagcgcatc
     5221 aggttagcac caacgtacac gcaccacgta gttcaggcga gtcttgccgc gcgcgggtac
     5281 cttgacgtcg aacttgaagg tgttcgcgtc tttgcgctcg gcgggctggc tcgaagagac
     5341 cacgttgtag tctccgctcg caggttccag gtcttcaatc gtgatggcgg tgtccttgtg
     5401 gttgcggaac tcgatctccc aggtgctctc tgagctgcac tcgccgagca cggtgtagcg
     5461 gacttgcttg cgatctgcga cgacgtcgaa ggcttcaccg agcttgatcc gcacgcgctc
     5521 gtcgcgcggt gtgtgatcga tcgagtcttc gcccacgaac tgttgggaac ccgtggagtc
     5581 cgccttgtac acgcgcacca cacccttggg cagcggcatc ccgagctggt tcttctgatt
     5641 gttctcgaac tccagataca cgcccacctt ctggtttttg cggggggtcg cgtcgggctg
     5701 acggaaccag taggtctcac cagagaaggt gaacttcttc tcgatgccga tgcccttggc
     5761 ctcgaccagc gcgacttgct tctgttcgtt gttgaggacg tcggttttgc gaccgagcga
     5821 gtagaggtgg tactcgagca atccctcctc cgcgaactgc gcggccctcg gggccgccat
     5881 cactttgcgg ctctccatgg ccaccatgtc ccgcgcgggc gcgacgcgat tcacgttacc
     5941 agcgaccagc ttgagatcgg cgtccttgta cgagctgccg gactggttca cgagcgtgac
     6001 ccagccgcgc aggtcggcgc gttgctcgtt gtcgtccagc accgccacgt aatccgcgct
     6061 ccagttcagc ccgcccgcga ggtacgtgac ttccacctct tgtttagcgg acttgccgtc
     6121 gagcagccag aacagcgttg gtttttcgat caggttctcg ggcagagtcg gaaaggcgaa
     6181 gcggccgccg tagtcgaacg tgatctcgcg attgatcgag agcacgggat tgcccgccac
     6241 gctcagcagg cgggcgtcgg tcacgtcctc tcggccagag ttctggtgat atcgatagac
     6301 ccgcacgtcc ttgccgacgt acttctccag caacgtttgt ggcgtgagta ggtcatatcg
     6361 ataattctgc tccagcacgc tcagcgtgcc cggccccgat agcgcgcgga tatgtacggt
     6421 ttcgggctgg attgtggacg cgacgtctcg atattcgagc gccatgcgtc cgatgggtag
     6481 cccgctgagc tgacgaacct cgcgcaccaa ccccaggtcc tggttgtaga cggtaaggct
     6541 gacgttcttg cgctgcgagg cgtccgatac cgcgctttgg gccgctgcta ggggagcttt
     6601 tgcggcagct tgcgccagcg ccgcgctcgt cacggaaaag cagattaaac ctgccacagg
     6661 gcccgagcca cgggaaatcc agctcgggat ggcagagcaa ttgagcgcgc gggttgcggc
     6721 ttggatcgaa cgtagcagct tggttttcat cgagagtccc atcagcaaga gcgcactcga
     6781 atccggtact ccacgcgcgt tgcgccgcgc gccggaaccg agagcacata agagaattgg
     6841 tcacgcgttt gccgcttggg cggcaagctc gtactcacga gctcgtagtc cgtgccgccg
     6901 gtgttttccc gtaattcgac ggtttcgggg ctatctttgt gattcttgat gtccaggcgc
     6961 caatcggtct cgagcgtgca gcgcgaaact tgggaacgct tcagcgcgac ccgctcgaga
     7021 acgacgtcga atgcctctcc cacgctgacg ctcaacgtct cgtcacgcgg agtatgttcg
     7081 atctcgtctt caccgatgaa ctgcgccgcg ccggcagcgt cccgcgtgta ggcgcgaaga
     7141 atgccttggg ggagcgccat gccgagcccg ttctgctggc tattctgcag ctccaacacg
     7201 acccgcgcgt gttccagcgg cttccgctcc gccggcatcg tggaagcgat taggaggcgc
     7261 ggcatgttcg ccgtgaagac cagctttttt tggacgccga cgcgcgcggt tccgaacaag
     7321 ctgacttgtt tgcgctcctt gtgacgaagc gtggtcgggc gatccagccg atacaaatgg
     7381 tactcggaga gcgcctcctc gcgtaagccc tgagtgtcag ccagggtggc attctccttg
     7441 taatcgtcgg cgtcttcttc acgcggggga gcgagacggt tcacctggcc tgccaccaac
     7501 ttcaaatcgg cgttctcgaa gccgacgccg ctttcgttgg tcaacgtgac ccagctcgcg
     7561 agctcacctc gggcgccatc gccgtccagc gagagaacgt agtccgcgtg ccagttcaga
     7621 ccgccgctca agtagctcac ttccagctgc tgccgcggcg ttccgctatc cagcagccac
     7681 tcgagcgtgg gctgactcac caggttgttc gggatctctg ggaacgccag tcgtccgaga
     7741 tccgttcccg aggtgatttg gccgtcgacc ttcagaacta cgcccttctc tgcggcgagc
     7801 acctgggcgg tctttacctc atcgcggccg agctgttcgt tgtagcggta aacccgcacg
     7861 ctcttgccga cgtacttctt cagcaggttt tcgggggaga ggaggtcgta gcggtagttc
     7921 tgctcgagga cttcggcctg gcccggatgg gagaccgagc ggacgtgtac gctctcgggc
     7981 tgaatcttct gggcgacatc ctcgaaggtg agtgcgacga gccccgaacc gagcgcgatg
     8041 ctccgtcgct cccggatcaa gcccaagtct tggttgtaga cggtgaccga aatctgctcc
     8101 cgatctcgcg aggtggagag cgcactgtga gccgctcgtg gtgccgccgc cggtttcggg
     8161 gagctacatc cgagcagtcc agcagcagcc aggctcgcga gcatgagacc ggcgcgtctg
     8221 ctccggatcc agccattcga aggaagtcgc atgcggattg tcattggaac gcgctcgtgt
     8281 gcggcacctt acacgtgagc ttacgagcgt gctatctcct cccgcgctgt gtccgcccgc
     8341 tcggaattca tcaaggcccg tcgtatcgtc gtcaagatcg gctcgcgatc actcatccac
     8401 gatggagagc tgatccgccg tctcagccag caggtggcgg aactcgcagg tcagggtcgc
     8461 tccgtgatcg tggtgtcgag tggcgcgatc gcgctcgggt gtcggcggct cggttaccgg
     8521 tcgaagccca aagaattggc gcggctccaa gcggcggcag ctacggggca gagcgtcctc
     8581 atgcgtcgct atgatgaagc cttcggcgag ctcggattga ccgcggccca ggtgcttcta
     8641 acccatgcgg atctcgcgga tcgcgaacgg ctgaacaacg cccgggagac gttcgccgcc
     8701 ctgctcgagg cctcggccat cccagtcgtg aacgagaacg ataccgtgtc gaccgaagaa
     8761 attcgcttcg gggacaacga ccagctggcc tcgatggtgg tcccgctggt ctcggcggat
     8821 gctctggtct tgttgactga cgtggaaggc gtcttggatg gctcgggccg gcgcatcccc
     8881 atcatgacgg gtgcggaaaa gctcgaactg attggcgaac ggcgcgagcg cgtgggtacc
     8941 ggagggatcc agagtaaggt cgacgcggcg cgcaaggcgg cccaatccgg agcgtcggtg
     9001 gtcattgcgc cggcgacccg gcccgatgtg ctgcgcgcgc tctttgccgg cgaggacatc
     9061 ggtactctat tccccagcag acagaacccg ctccgtgccc gcaaacactg gatcgcgttc
     9121 accttgagga cgcgcggcac tctcttgctg gacgagggcg cgacccgggc cgttcgtgca
     9181 gggaagagca gcttgctgcc aatcggtgtg atgggtgtcc gtggcgcgtt caaccccggg
     9241 gatgccgtcc gcttgattgg gcctgacggc gcggagatcg ggcgcgggct gacccgactg
     9301 ggggcgttgg acgcggcccg cagcgccggc cgtaagggca ctgagctcga tctgctcttt
     9361 ggatccggtg gcgcggactt ggtcgtcgta catcgtgatg acctcgtggt caccgactga
     9421 gcgcgtctat cgtttggagc tcccttggag ctccctcgaa agtatggcgg cggtattggt
     9481 aacgagttcg ccgcaatgga atccaggcta ggcgcagcct ctggtggatt tcgacgccag
     9541 tttcgttact ccgagccaag ccaaaaaaag ttccggggtc cgaaacttcg gaccgggtct
     9601 ggtgtcggag tggctctgga cgtttggtct tcgagagctc cgattggcgc ccgcctccat
     9661 cctgacagct tggtccgcgc gaacctgggg gtcgacgtgt gcctggtcat cgcgcttggc
     9721 cctgtccatt gccggatgtg ccctgactgt cgcttgtgct cgcgggcctg ccgcgcccgc
     9781 gttggtgccc aaaccggcaa gcgcactagg agaagagcgg gggcccgcag tctcggaaga
     9841 cggcgttcca caagcgctca cggcgcccga agccagcgat tgggtgagtg aggatgactt
     9901 caccgtgcgg gtgattacgg atgcgattac ctgcacgggc gcgctgattg ctccggactt
     9961 ggtgttgacg gcccatcatt gcgtggcggc gcgctcgagt cagggggaca tcgtcgagcg
    10021 agacgtcacg agctcggagg tgaccgtcga actgggtggc gaccgatttc ctttgggaga
    10081 ggccggtgtg cgcagcgtgg tcagcccccc ttgcggctat tctgggggcg ctggcgacct
    10141 tgccatcctg gtgctcagcc gcagtctgcc gggattgccg catcgagcgg tggagctcga
    10201 gcggatgcct agagcggggg atagcgtaat tccgctggga tttggccggt gcgcggggac
    10261 caacgacggc atctatcgca aacgccgcgc agccacccca ctgcacgtcg tgcaagaggg
    10321 ccgcatgttg ctcgatgccg cgatttgccc tggagattcg ggtggcccgg cgcttagctc
    10381 gagtacgggg cgtgtggttg gcgtggtgtc ccggagtgtg atggatgccg acgaagcaac
    10441 cctggggttt acggagctgt cccgtttgga cggctttcgg gagttgttcg cggcggcagc
    10501 gcaggtcgcg cgcggcacga gccgagcaga attgcctccc atcgagtgcc cagtgatggg
    10561 gggacgtacg agccagcgct gaacgtctgg cgtgtacgtc ctgagcgctt ttcgcttcgg
    10621 tgctaacccg aggtccgggg ttgccgttcc aggtagagac actctcctgt ctctcgtctg
    10681 cgagcggact ccatgagcaa gttgcttcga ttcctggttt ggatgggcat cgctgccggt
    10741 attacggtgg gtatcagtcg cgccgtcgcg ctccgatggt ggaaggtgcc gagcggcgat
    10801 ccctatctcg aagcctcggt cgcgcccacg ttgcggggag gcgattgggt gctgctttgg
    10861 agattgagcg cgccacggct cggagatttg gtgctttgcc ccgagcccaa cgcgccgggg
    10921 cgcgaagttt tggctcgcat agccggtgtg caaggtgatc gcgtcgaatt ggatgggggc
    10981 atgttgcgga tcaacggtcg caaagccgcc gccaaccatc cgtgtcccga ggaccacttc
    11041 accactatcg atccggagac acgcgcggcg actccacaga gttgcgtgat cgaagagctc
    11101 gggagtcgca atcatccgcg cggtgaggtg acgagccagc tgcgagccgc cgaccacaag
    11161 agcttcgaag tgccggccgg gcaggtcgtg ctgatcagtg ataaccggca attcccttac
    11221 gactctcgcg atttcggact ggttgatcgg gcgagttgtc gggagagcgt attcttcagg
    11281 ctggtgagcg cagcggggtt ttccgacgtg gcgacccggc tcacctggat cccgtgaccc
    11341 cgacggcagg ctcgtacgca atggcgttgc aggaccggcg cctcggcata attctaagcc
    11401 gggcagcgaa tgaattggcg actggtcctc ggcttgtgtg tgctggcttc gagcggctcg
    11461 ctctacgcgg cgggggccgt ttggggagcg cggatcgtgg agcaacgcct gcgtccactc
    11521 aacgtttggg tggatggctg ggtgctcact ccgggggcta acgaaggcgc gctcgtcgag
    11581 caactcgcgc ggcgtacggc tgagcggcgc gcctttctca aggacggtgg cgagctttac
    11641 gggatttctt tcggggagct aggaatcgag ctcgatcggt ccgctttatt ggaacgcttg
    11701 cgcgcggctc ggccggacaa cggctggcga gcgcgttggt ctcgaatctg gcgagcgccc
    11761 gggccggagc ccgaagttcg aacacggctc gcgtgcgacg aagagcgcgc ggctcgggtc
    11821 ataacgagct atgcgtcggt cttggctcgc gcaccggtca acgcgcggct cgatttggcg
    11881 gcgcaccgcc gcatcgagga ccagcccggg cgtgagctcg acgtgccggc gaccttgaac
    11941 gagctgcgcc aacgagcatt cgacgggacg ccggtgttcg acgcccgttt ccacgagctg
    12001 ccggcgcgcg ttcgctccga gtctctgccc cccgtcgatg tgtctcgcgt gctcgcgagc
    12061 ttcgagaccg acttttccaa gaagccgatg tctcgacggc ccaatatcga ggcggcggcg
    12121 cgttacctgg agggcttcgt gctgcagcca ggcgcggtgc tgagcttcaa caaagtcgtg
    12181 ggcccgcgca cgttcgagcg cggcttccgc gaagcgcccg tgatcgtggc ggacgaacta
    12241 gagagcgact tgggcggcgg cgtgtgtcag gtggctaccg ccgtgttcgc ggcgtcggcg
    12301 ctgggcggct tggagatctt ggagcgccgt agtcatagtc gaccgagcgg ttatgcgccg
    12361 ctcggtctcg acgccgctgt ggtttacggc aaggtcgatc tacgggtccg caacccatac
    12421 ccagtgcccg ttctgctcca cacgtctttc ccgaccccga agagcctgcg cgtcgaactc
    12481 ttgggcttgc aggcgccgca gcaggtcgaa cactcgtttc gagttcgcga gcgcaagccg
    12541 tatcatcgca aggtcgtgat caagaccgat gttccgccgg acgcgatcga gcagaaacag
    12601 aagggcaacg cgggctacga ggtggtgagc acactgcgca gtgttgcttc ggacggcagc
    12661 gtgcgcaggc gcgaataccc gagccgctac taccccgtgc ccgaagtatt ctggatcggg
    12721 acggcggtag atccgaactc actgccggcc ttggcggagg acgaaactca cgtagagatc
    12781 gaacgcgcgc cgcaggcttt gtaggacgct tacgctacga cgttcaggcg ttgaccgacc
    12841 ccgttcgcca cattggacgg cgcggacgcg gcatcgatca acttcagagc gttctgtcct
    12901 tgttgctcga cgttgtcgag cgctttgcgc gcgactttca cgtcgaattc ggcgcgcatc
    12961 tgcgctgccg tgagtgcgct actggcggag ataccattgg agcccatctc accccccgag
    13021 aacggtgtgg cgtgacggac cttgaactct tttttcgaat cggtttccca gattcgattg
    13081 gagtcgccgc ccatactcgt tcggcgcgtg tgggcgcgcc cgcgggttgg gtcaaaagtt
    13141 ggccacggcc gtgcgtgcgc gcgaggatcc caggatgcac caatcttgcg aggtttgcgc
    13201 ccaattcctg cctgcgcgcg gggtctcgaa gattccggat ccaaccgcgt gggttccttt
    13261 cgttcaggcc gatcccaccg atccccgaac cgtcgcgctg tgccgtactc acctgcacct
    13321 ggtttgggcc gcgggtgcga agacctggtc agagctcgta gagttgtttc gcgagaacga
    13381 ggggcgccgt tcttggttgc cgcgtagaca agtcacgcgg acggcccatc caggccggcg
    13441 cgcgacggat cgggcaaagg acccaaccgg ataatttcga agagtacagg ttgacttttc
    13501 ggagccgttc gccaatctaa gggcctcgtg tcgtcgtcgc tgtctcttgc tctcctcgct
    13561 gggctgcttc ttagcagcgc gggcgaggtc tgagcgcgca gcaccagctg acacgacgct
    13621 caataatcgg acctctcccg ccaacgagcc ggagaggatg tggtccgtcc agtttctccg
    13681 gcgttcatcc cgagagaaac tgcaatggcc tacaatccaa gacgttaccg agcacccgag
    13741 acgattcgtt tatccgaccg cacctggccg gagcgcacgc tggagcgcgc gcccacttgg
    13801 tgcagcgtgg atctgcgcga cgggaatcaa gcgctcgcgg agccgatggg catggagcgc
    13861 aagcagcgga tgctcgcggc gttgctggag gtgggcttcc gtgaaatcga gctgggcttt
    13921 ccctcggctt cacagaccga cttcgatttc atccgttggg tgatcgagga gaatgtgttg
    13981 ccggagggcg tcaccccgca ggtgatcacg caggcccggc cggcgctgat cgagcggacg
    14041 ttcgagtcca tccgtggtgc caagcgcgcc atctttcact tgtacaactc aacctcggag
    14101 gtccaacgcc gcgtggtatt cgggctgtct gaagcggaag tgatcgcgct ggccgtgcgc
    14161 gcgaccgagc agattcgggc gctggcggcg caaatgccgg agaccgagat catcttccag
    14221 tactcgcccg agagcttcac gggcacggag ctggcgttcg ccaaggcgat ctgcgacgcg
    14281 gtcgccgaag cgtggggcgc cacgcccgag cgcaagatga tcgtgaacct gccttctacc
    14341 gtggagaaca cgactccgaa cgtgttcgcc gatcgaatcg agtggatgtc gcgcaacttg
    14401 gcccggcggg acagcatcat cctgagcgtg cacacccaca atgatcgtgg ttccggtgtg
    14461 gcgagcgcgg agctcgcgca gttggcgggc gccgaacgcg tcgagggcac ggtattcgga
    14521 aacggcgagc gaagcggcaa tgtggacatc gtgacgctgg ccttgaatct ctactctcat
    14581 ggcattgaac cgcagctgag gttcgatgat ttaccggcac tggtgaaggt catcgaatac
    14641 tgcacgcgcc tgcccgtgca cccgcggcat ccctacgcgg gggagctggt gtttacggcg
    14701 ttcagcggct cacatcagga cgcgattcac aaaggcatga agcgtgtagg cgaggggccc
    14761 gacgatattt gggaggtgcc gtacttaccg atcgatccga tggacgtggg gcggcgctac
    14821 gagagtgtga tccgcatcaa cagccagtcg gggaagagcg gcctcgcgca cgtgttggag
    14881 cgtgacgcgg gctatcgcgt accgcgccct gtggccatcg agttcgggcg cgtcgcgcag
    14941 gcggtcacgg acgggcgagg ggaggagctc agctcggagg gcgtcgtggc cttgttcgag
    15001 cggacctatt tgagtgtttc ggggccattc gagctccgga cggtggccgt ggagcggcgc
    15061 tccgcgggtc gcgattgcgc cgtcacggcg gagctgcgca ttgacggcgt atctcgcacc
    15121 gtgaccggga tcggcagtgg tcccatcgaa gcgttcgttt ccgcgctgag cacttgcgtt
    15181 gggcagacgc tcgaagtggc tgactacgtc gagcatgcgc gcggccgcgg cgccgacgca
    15241 gaagcggtgg cgttcgcgag cgtgcgcggc gctgctgacg cgggcgagcc gctgtacggg
    15301 gtcgggcgcc acaaagacgt ggtgctcgcc tcattgcacg cgatcatcgc cgcgtgcaat
    15361 cggctccacg cggcatcacg gttcgcacaa cgcggcccaa ccgtcgtcgc agtcgacatc
    15421 gctccatagg ccgggctcca cgacggcgcg gctgatcacc atcaccgcgc aatcgtcccc
    15481 gccgcttcgg tccccggggc gctctgcgcc ccagcccgag aaagcgccgc caacggggct
    15541 accaccggcg gcgggagtcg ctccggtcca gaacgcttcc ccgggttgcg cgcgctccgt
    15601 cacccattgc cactgccctt ccttggctga atcggacgct ccgagccaga ctcggcgaag
    15661 ctccggatcg tgcttcatga tcagctcgaa cgcctgcttg ttcgccgcgt ctgatttcag
    15721 ccaagctagg tgcatgcctg cggttgcgca gagctttgcg gcggcgtcga agtcgagctc
    15781 ctgcgtgcac aacatccaag agtgctcgga ggaaccgagc ccaacgcagc tcgtggggca
    15841 cagctcgccc tcatcgatgg cgccatcgca gtcgttatcc agtccgtcgc agcgttcttc
    15901 gccccgcagc gcattcggcg tggagcaatc gtctcctccg gagccgccca tatcctgacc
    15961 cgcgctaccg cctgcgccgc cgctcgaaat aaggccgcca ctgccctgcg gctggccgcc
    16021 gctcgtgggc gctccaccgg gatctgcgcg gccgccgctt tgtatcgcgc cccccgaatg
    16081 gggcgcaccg gatccaccgc cggagtttcc cgttttgccg ccatcgccat tgggtgaggt
    16141 ctcgctcgac gagaggccgc ccgaggacgc tcctccgttc cccgtcccgc cgctctcact
    16201 caagctcgtg ccgccgctcg acggactggt tccggacccg atcgagtcag tcgagcttcc
    16261 tcccgacggg gagctgactc cgcctgcgct gctgctcgtt gcaccgccgc gcaccccgcc
    16321 cgccgactca ttgaatagct cgggtgcggt ggggccgtca caagcgctgg cccaacccgc
    16381 acatgcagca actgacagca gcgcgagcag accgccagcg ttcctcgtct tcacgtgaat
    16441 cacactccaa gtattgaagc tcgcgctgca tacaagcaaa gagttatctg cagtgggctg
    16501 cgcgatgatc gagtgcagta gactctcgta ctcaggttgg cgtactcaac ggggttgctc
    16561 tctcgcacgc gccgcggtct cacaccatcg tgttgattcg acgatgattc tctgatcgct
    16621 tttccgcctg cctcacgttt ggtggagcgt ggcacccgcc aaaatcacgg agtttctcga
    16681 gttcctgatt cgtatttgtt tggagcgtga ttttcgacga gagcccgtgc ttttcgaggc
    16741 acggggttac attccgtgcg tctcggtcga tcgcgtgggt tccgaagacg tcgcggctcg
    16801 cgatggcact ggaggctgca atcgcacagg acgcgggctc ggtcgtcgaa gcgcttgcag
    16861 ctctgcgcag tcgcggtgaa tcagagagtc acgactctga cgcggatctc acggcggcaa
    16921 acatttgcga tctcgcacga agcggagtcg aatcgcccac gccggtctcc aatctccgcg
    16981 cgttttttca ggctggcggc gcaacgggtt gccaaatagc cacgggtgct atgttcgcgt
    17041 cggcatggtg accacgactt cgaacgagca tgcggaccgc agcttctttg tggcgaacgg
    17101 cgtggtctct gcgctcgctc tgtcgttgat cgcgttcatt cttttgcgcg acggcggcgg
    17161 agctcaggac acgcgactcg cgttcgttcc ggcgctgaat gcagtattca acgcgctgtc
    17221 ggcgacctgt ctcgtgttgg gctatgtcgc gattcgccga ggcgcacgtc gctcgcatca
    17281 acgcttcatg atcagtgcgt tcgcgctctc cagcctgttc ttggttgggt atctggcgta
    17341 ccactccgtg cacggagata cgaagttcac cgggacgggg ccaatccgtg cggtgtactt
    17401 tttcgtcctg atcagccaca tcgtgctctc gatcggcgtg gttccgatgg cgctgacgtc
    17461 cttctatttg gcgttcaaac agcagttcgc gcgtcaccgt cggctcaatc gcgtgtttct
    17521 cccaatctgg ctgtacgttt ccgtgaccgg cgttttgatc ttcctgctgc aacggctagc
    17581 cgcttgagcg cccgatagtt catcggcgtt gcgctcgaga gccagcgtgg agacgagacg
    17641 aagcccacgg ccgaagcgac aaaaagaaga cgagccgacg cgacccttga ggtcgacggc
    17701 tcgattggaa actgcgtcct gggagcaggg ctcgccttgt ccctgagttg cccaataccc
    17761 cgcgcaccat tgcccggttc cactgctcca ccacccggag ggcccggctc ccaggacgct
    17821 tcgtctcgtg ttgtctacga attcattcgc ttcaggcgtg cccccgaaat cggatccgac
    17881 cctgctcagc cccgatcccc gacttggcca cccgatgccc aacccgcaaa ccccatgaac
    17941 gccgtgcccc gtcagccccg ctgctcccat aggccacccc gtatcgcggc cctgactcga
    18001 ccccgcgcct cgattgcccg gcgccccgac tgctgcccct tgccctgagc tccacgtgcc
    18061 ccgattgctc ccagtcccat ccccgtcccc cgctgaatgc cccgagcccc gctgaatgcc
    18121 ccggccccgc tgaatgcccc gcttcttggc cccgactctc cccgcgcgcc gtgttctccc
    18181 cgcgcgccgt gttctccccg cgcgctgtga cccctgggtc cccatttacc ccgttcgact
    18241 gtcttgatcg gcccgcgctg attcgcgagc ccgctggtgc cccgacggca tgctgccctg
    18301 acagaaaatg ccccgagtgc cccgaagaat atacgcgccc tgagggcagg gtgacttcta
    18361 cccccgagcc atcccgcaat cgggaagact cctcacccca aggaaccctg cccccaggac
    18421 gcgacgcaga attctcagcc ccagcttggc tgggcgctcc actggagagg gcactggctg
    18481 tcgaacggat tgctccgtgc ccactggcgc gaagtgacgg tgagcgcgac gaggaagcgc
    18541 tgttttccgt gacttggctg agactcatgc ttgcactccc ccctagtgca ggctgcgtgc
    18601 caggcgtgat acagcggatc gcggtgtcgg ccggctggga gagtcgatcg attagtcggc
    18661 aaaaatgccg ttaaaacggg ctctttcgag ccgtgacgat gttcctgaat ccacgcaggt
    18721 gtatcagccg agtgatacac ctgttggaca gatcctggag acactggatg cgtgtccatg
    18781 ttccagggct ggtccgtgat cggcggttgg gtataggctc cgcgcctctt ggaagtctct
    18841 gcttcttcag cggcaccggc tcaggccttg cgcgtggctt tcgtcgttca gcgctacggc
    18901 ctagaggtca atggcggcgc ggagttgcac tgccgggagt tggcggaacg cctcgcgcgg
    18961 cgcccggatg tagcagaggt cagggtactc acgacctgcg ccaaacgtca cgtcgattgg
    19021 cggaatgact actcgcccgg caccgagcgg cttggtggtg tcgtcgtcga gcgcttcgtc
    19081 acggatcgcc cacgagccaa gctcctgtcg gagagcttgc aatggctgct cgcgcatctc
    19141 ccgcatcctc gcgggttgga gcaaagctgg ctcc
//
LOCUS       DASTKU010000639        10029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_122922, whole genome shotgun sequence.
ACCESSION   DASTKU010000639 DASTKU010000000
VERSION     DASTKU010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122922"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..711
                     /locus_tag="VFQ61_22375"
     CDS             331..711
                     /locus_tag="VFQ61_22375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957268.1"
                     /translation="MANTPGIHTSDFHIGRYRLAKHFATSAINALDVDPKYARQLLLE
                     SASEFPSGRVPLFVCECCADLSCGAITVAVEIADGLVTWRDFGRETPYASVPAFPEIY
                     ARTGPFYFELTAYRSTLRPYSHTR"
     gene            complement(693..1118)
                     /locus_tag="VFQ61_22380"
     CDS             complement(693..1118)
                     /locus_tag="VFQ61_22380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957269.1"
                     /translation="MYRVVRVDEPAHRITVVNEDGGRELMLDGLVLEAQSAMSDGQTL
                     LWVTDGSPYEEGLHIYLIARTGEVIDVIEAGAPYSPGLLEILSTSEQSVQFRFFRPEV
                     IYRLEVAPLARLRWFLPNGFRYERTLSRHFLTVVQRVWE"
     gene            1183..1917
                     /locus_tag="VFQ61_22385"
     CDS             1183..1917
                     /locus_tag="VFQ61_22385"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET9957270.1"
                     /translation="MVTCSAFPYAFQTDGVLRVDAALASKGLARLWSQLAPETASGAV
                     STRSGSSAPGVYAARALLLSRPQLPSILAELGMDEVANRALGTAGFPIDALFFDKGAD
                     ANWAVPAHQDIVVPVPPNSVGLSVRNSRMRHGTFYGEPPLQALRELVALRLHFDDCSR
                     STGGLAVIPGSHDRRWSDAQLAGIPSDAFRHCDCRAGDVLLMKPLVVHRSPRAEQPQR
                     RRVLHVLYAPTDGWHARLSGYTGMAW"
     gene            1960..3048
                     /locus_tag="VFQ61_22390"
     CDS             1960..3048
                     /locus_tag="VFQ61_22390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957271.1"
                     /translation="MLLARTFALARAEDAAEPRARSNAAAQEIGLEYEVPAGCPGIDV
                     YLTDVAQRLGPGWHAALKDLARHLTVTVTHVGERYTGSLDLVTRRGERFYRSVNGAVC
                     SEVVLGIGLITELAAKARASEPAGAIGTSSDTGAAAAADADRDTDARAAPAPSPLSST
                     PQSESGVRPASALRAKRNTAVPPVQGRVYGKAKIRFGARASLTSGVGPSVAMGPGVFA
                     VLELDRARFGLSADLHTSGEVQARRVRADFRLLSVRLDGCPWSFRLGDWGAAEPCAAA
                     ELGSFRAAPEIEPPIVTAAAPQSIAWGAVGLLGRFVLRFRPFVAQVELIGRAPLRRES
                     FFVGSREDVVFRIPVVSAGASAGLGLEF"
     gene            3055..3699
                     /locus_tag="VFQ61_22395"
     CDS             3055..3699
                     /locus_tag="VFQ61_22395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9957272.1"
                     /translation="MSLPVDFQIESVCPGDEPAVPVGSPSPALSANAADVRVGADSKR
                     IAAFVREYHGFAWRVLRRFGLAPADADDAAQRVFMIAASRLGDIAQGSERAFLFRTAA
                     HIASKVHRARRQRPDPPSDHALESADPRPLPDALLEQRRARDLLDRILGELPQELHAV
                     FVLFEIEGLRAPEVADALEIPLGTVASRLRRARAEVSERLARHQAHSMFKGRLP"
     gene            3696..4514
                     /locus_tag="VFQ61_22400"
     CDS             3696..4514
                     /locus_tag="VFQ61_22400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9957273.1"
                     /translation="MNDLKPWIEDGAPEAFTELLEAARVEQPSAESLRRSLSAAGAGV
                     VTASTASAMAGATASGLVQTKGSILLTTSVLKWMLVGVGLGGAAVSTVAEVRGRARQL
                     PATAVSALSPSSTRASGSGAARTGTRELPTHTSLLAPASSLTSHGVDSSKIVTPPAEK
                     RVPNDSPAREVPARLPQKTESERLADEIRAIERATRALASGQAAQTLALLDDYDRRYL
                     ERRFGPESLFLRMEAFAKAGRHIEARTVAERLIATYPNSPQSTRARAVLSSTIP"
     gene            4582..5736
                     /locus_tag="VFQ61_22405"
     CDS             4582..5736
                     /locus_tag="VFQ61_22405"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HET9957274.1"
                     /translation="MKHSITLASLTIGLLGTACGGYTKIGEGSGGSTGTPTGGAAGGN
                     GGRSYGGTANRGGAAPTGGTSSARGGSTFGGSTSRAGESGIGNATGTGNTTGAGGATG
                     MSCNTDRDCPRSLVACQLCADGTTACPWAVCENGTCRSGIDRCPPPSPCAGKACGELC
                     TTCSDEQPCPPVLMFCDEQGQCQRDEPTCKAPLCKTDTDCPEVEACPPCSDGNCAEYK
                     CVSGACQFACESQGGSGGAGGGTCSAQGASCANGETCCAGLECCAGVPVPEGNEYCGT
                     VCPKSDVNIKRDFLSVDPNQILDKLASLPVGTWAYRTEGSTERHIGPMAQDFKAAFQV
                     GSSDRTILQVDADGVAFAAIQALNARLKTLEEQNRKLRSDLDGVRQRCAP"
     gene            complement(5741..9253)
                     /locus_tag="VFQ61_22410"
     CDS             complement(5741..9253)
                     /locus_tag="VFQ61_22410"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957275.1"
                     /translation="MRQLHFRVFARGARGLGWFVGCLGSCLGCSLELHASGEFAGMSG
                     AGQTGAGAVPSSGGRETGGQTGTSTGVTSGSGGISAGNSSGGASAGGSSTSGGDSTFG
                     GSGGDAGGTEADGGTASGGTAGNTTTGGASGTGGEPTISAQPCGIVDLGPDTLIDLGH
                     TAGIELARVSANRGLALDSTGHWLLWDLASHVRIASGDITQRDCPLRGKDCAFGLAGD
                     TLLVPKQRTLEVRSALDASLISSITKPEPEILPSEWGGFVPRSVEVGLAIDGSYLWTR
                     SSKALTVISLSGTVLFEHSGNYLDARLVAAPQGIRVAKGPVASNVIELISVPTGQSSS
                     SSPYLGEFHSWFADGTKFLTSVGSVVRVYDQDANLMHPLTILSSVGNLAGYGDYFWTY
                     EDFSSAEPLKIYRLGIAEPVATFPPTGSTYHVVPSEGSLGLVPWADAPFSVLDLTRSS
                     VEQVPYSLPAAYVSSFASDASGRWIAGTAGGAVLNGGRLNQPLAPRPLSCGAVLSMAG
                     SSAGRVVFATAAGQLLSFDVESHPTFERALAYPTGRLELSQDGQVVAALGTRFGDQYL
                     GDRELSVFSLPSMRTISSWKYEDPNGPTPAYDFALSPVGTRLAQQNCRYEKDPLSGVG
                     YTAHCRHMLTSLDGSETYFAEDSAYEFILPLSISPNNAQLAVGSPTGPVDPQGAKTTN
                     TLIYRNGMLIGVTEGSPATWVDDERLLVYGRSGGLILTNAEATSSTPVSIPAIGRGTW
                     FPPLERPYDVIDGERIYVRSERAVYDLSDGKVSFRLNDRSPGPSAAVGRRVAYTSTRR
                     VLTTELSGTGGPGAPTPVSAVTDLATSGNSTCARLADKSVRCWGQNDFGALGTGDATQ
                     YSASPRDMILIHQVEQVSLGGPFGCALNSDYRAYCWGLNNSGQLGAGVSVEASLAPLP
                     VVMLGDATQLALGSEHACALLQNGSVSCWGSNWRGQLGDSTSANTNTPIEVPSLSNVV
                     SLAASSLQTCAVLTDGTARCWGDNERGQLGNGTSLSPGLPMPVVSLSRVRQIAVGSGH
                     ACALLSDGGTQCWGANEFGQLGDGTNQDRPVPTPIPELTNVVQLSTSESHTCALTADH
                     SIYCWGMNGSGQLGDALPHSKPTRIAFVSEAVQVAAGAQHTCALRSDRSVVCWGDNSR
                     GQLGDGTTQYRSDPVVVQGLELAE"
     gene            complement(9425..>10029)
                     /locus_tag="VFQ61_22415"
     CDS             complement(9425..>10029)
                     /locus_tag="VFQ61_22415"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9957276.1"
                     /translation="TIHPSWWSCPRCQDIILGSQDGAADIAVIEVAEDTPAIPQARVE
                     LGIVGVDTPLVKVGWGCEERTNVDPSTVKLGRFKAANARAISASEIRRFDSRISDEQV
                     AMVDASYVITAGHDQDPQNASLCLGDSGGPLYLPNNGDPRVVGVNSDYTFKPPEDDSN
                     LGGVSWTDWHTRTSLGSFLGVGEWLAGLGVNTVNSQSSQP"
BASE COUNT         1857 a         3083 c         3207 g         1882 t
ORIGIN      
        1 tcgagcgata gagacatcat cgtcgcgcga ccacgcggcg ccccttgaaa gctcggactg
       61 cgaatccggg ctttcgcctt atttggcgga cccaaccctc cttagccggc gttagcccat
      121 gcgggccact ttggtgcgga aatttggtcg tctgccgcgc tttcagccgg aattgcgcgg
      181 attgggagag ccagcgacac gaagaccgaa tgctagagga atcattgtca gagtatcgga
      241 agtataaatg tgaaacgaag atgctcgttc cgtggttctg ctgagcagga ttgaagcggg
      301 tgactgggta tgaagttttc tgtccttcgc gtggcaaaca ctcccggcat ccacactagt
      361 gacttccaca tcggtcgcta tcgtctcgcg aagcactttg caacttcggc gattaacgcg
      421 ctggacgtgg atccaaagta cgctcgtcaa ctcctgctcg agtcagcgtc cgagttcccg
      481 agtggacgcg ttcctctctt cgtgtgcgag tgctgtgccg acctcagttg cggtgcgatc
      541 accgttgcgg tcgaaatcgc tgacggcctc gtcacgtggc gagacttcgg tcgagagacc
      601 ccctacgcaa gtgttcctgc tttcccagaa atctatgctc gaacgggccc attctacttc
      661 gagctgacgg cctaccggtc gacactgagg ccctattccc atactcgttg aacgaccgtc
      721 agaaagtgac gcgagagggt gcgctcatac ctaaaaccgt tcgggagaaa ccagcggaga
      781 cgcgctaggg gcgcaacttc gagacggtaa attacttcgg gtctgaagaa ccggaattga
      841 actgattgct cgctggtgct cagaatctcg agcagccctg gagaataagg cgcgccggcc
      901 tcgatgacgt caatgacctc gccggtacgt gcgatcaggt agatatggag tccttcttca
      961 taggggctcc cgtccgtgac ccaaagtagc gtctggccat cggacatcgc agattgcgct
     1021 tcgagaacca atccgtcgag catcaattcc cgacctccgt cttcgttcac caccgtgatg
     1081 cggtgagccg gttcatcgac gcgaacaacc cgatacacga gtaggtacta acacatcgga
     1141 cgcgctagcg acagatatgc tagcgttgct tgccgccaag atatggtcac ttgctctgct
     1201 ttcccttacg ccttccagac cgacggcgtg cttcgagtgg acgccgctct tgcttcgaag
     1261 ggactcgcac gtctttggtc tcagttggcg cctgaaaccg caagcggagc ggtttcgacg
     1321 cgctctggat cgagcgcgcc tggggtctac gctgcgcggg cgctcttgct gtcgcgccca
     1381 cagttgccct cgattttggc cgagctgggc atggacgaag tagccaatcg tgcgctcggc
     1441 acggctggat tccccataga tgcgctgttt ttcgacaaag gcgcggatgc gaattgggca
     1501 gttcccgcac atcaggacat cgtggtgccg gtgccgccaa acagcgttgg cttgtccgtg
     1561 cggaattcac gcatgcgtca tggcacgttc tatggtgagc cgccgctgca agcgctgcga
     1621 gagctcgtcg cgcttcgcct gcacttcgac gattgcagtc ggagcactgg ggggctggcc
     1681 gtgattccag gaagccatga ccggcgctgg tctgacgcac agcttgcggg aataccgtcc
     1741 gatgcgtttc ggcactgcga ctgtcgagcc ggtgatgtgc tgctgatgaa gccgctggtg
     1801 gtgcaccgat ctcctcgtgc cgagcagccc cagcggcgcc gcgttcttca cgtgctctac
     1861 gctcccacgg atggttggca cgctcgtctg tcgggttaca cgggcatggc ctggtagatg
     1921 aacgtcgctc gtactctctg ctcagcgctt ctcgtcgtcg tgctactcgc gagaacgttt
     1981 gcgctagcgc gcgcagagga cgcagccgag ccgagggcac gctccaacgc ggctgcccag
     2041 gaaatcgggc tcgagtacga ggtgccagca ggctgcccgg gcatcgacgt ctacctgacg
     2101 gacgtagcgc agcgccttgg cccgggctgg cacgcggcct tgaaggatct cgctcggcac
     2161 ctgacagtca cggtgactca cgtcggtgaa cgatatactg gctcactcga tttggtcacc
     2221 cgtcgcggag agcgcttcta caggagtgta aacggcgcgg tatgttcgga ggtggtgctt
     2281 gggatcgggt tgattaccga gttggccgcg aaggcccgcg cgagcgagcc cgcgggtgcc
     2341 atcggtacga gcagtgacac cggcgccgct gccgcggccg atgccgatcg agatacggac
     2401 gcgcgcgccg cgcccgctcc ttctccgcta tcttccacgc cgcaaagcga gtctggtgtg
     2461 cggcctgcgt ctgccctccg cgcaaagcga aatacagcgg ttccgccggt gcaagggagg
     2521 gtgtacggca aggctaagat tcgctttgga gcgcgtgcgt ctttgacgag cggagttggg
     2581 ccgagtgtcg cgatggggcc gggcgtcttc gcggtgctcg agctcgatcg cgctcgattc
     2641 gggttgagcg cagatctgca tacctcggga gaagtgcagg cgcgcagggt tcgagcagac
     2701 tttcgtctgc tctcggtacg tctagacggc tgtccttggt ctttcaggtt gggggattgg
     2761 ggtgcggcgg aaccttgcgc ggccgccgag ttgggctcgt ttcgagccgc gccggagatc
     2821 gagccgccca tcgttacggc tgcggcgccg cagtccatcg cttggggtgc agtcggctta
     2881 ctcgggcggt tcgtgctccg gtttcggccc ttcgtggcgc aggtggagtt gattgggcgg
     2941 gcgccgctac ggcgagaaag cttcttcgtg gggagccgcg aggacgtcgt ttttcgaatt
     3001 ccggtggtga gcgctggggc ttcggcaggt ctgggtttag aattttgacc cgatatgtcg
     3061 ctcccggtcg atttccagat cgaatcggtt tgcccggggg atgaaccagc tgtgcccgtt
     3121 ggctccccgt cccctgcgtt gtcggcaaat gctgctgacg ttcgagttgg ggcggattcg
     3181 aagcgcatcg ctgcgttcgt gagggagtac cacggcttcg cttggcgcgt gctgcgccgg
     3241 ttcgggcttg cgcctgccga cgctgacgac gccgcccaac gggtgttcat gattgcagcc
     3301 agtcggctcg gggacatcgc gcagggttcg gagcgagcgt tcctgttccg cactgccgcg
     3361 cacatcgcct ccaaggtgca tcgagcgcgc cggcaacggc ctgatccgcc gtcggaccat
     3421 gcgctcgagt ctgcggatcc aaggcctctt ccggatgctt tgctcgagca gcggcgggcc
     3481 cgggacttgc tcgatcgcat tttgggcgag ctcccccagg aactgcacgc ggtgttcgtg
     3541 ctcttcgaaa tcgaagggct gagggcgcct gaggtcgccg acgcgctcga gattcctctg
     3601 ggcacggtgg cttcgcggct gcgccgcgcg cgtgccgaag tgagtgaaag gctcgcgcgc
     3661 caccaagcgc attcgatgtt caaaggacgg ctgccatgaa cgatctcaaa ccctggatcg
     3721 aagacggcgc gcccgaagca ttcaccgagt tgctcgaggc cgcgcgcgtg gagcagccgt
     3781 cggctgagtc gctgcgcaga agcctaagcg cagcgggtgc gggtgtggtg actgcgagca
     3841 cggcctccgc tatggcgggg gccaccgctt ccggactagt ccaaaccaaa ggctcgattt
     3901 tgctcaccac gagcgtactg aagtggatgt tggtgggtgt ggggctcgga ggggcggcgg
     3961 tgtcgacggt cgcggaggta cgggggcggg ctcgacagtt gccagcgacc gcagtctcgg
     4021 cgctaagccc gagctctacg cgagcaagcg gatctggggc ggctcgcacc ggaaccagag
     4081 aattgccaac gcatacttca ctgttggcgc cggcttcgag tctgacgagc catggagtcg
     4141 actcgtcgaa aatcgtaacg ccgccggcgg agaagcgagt gccgaacgat agccctgcgc
     4201 gggaggtgcc agcgcggcta ccgcaaaaga ccgaatcaga gcgattggcg gacgaaatcc
     4261 gcgcaattga gcgcgcgacg cgcgcactcg cgagtgggca agcggcgcag acgttggcct
     4321 tgctcgatga ttacgatcgg cgttacctcg aacgccgctt cggaccagag tcgctctttc
     4381 tgcgcatgga agcctttgca aaggccgggc gtcacatcga agcgcgcacg gttgctgaac
     4441 gtctgatcgc tacgtatccg aacagccctc aatccacgcg cgcacgggcg gttctttcgt
     4501 cgacgattcc gtgatcgaag ttgggtcgcg gagggatgag ccttcgaacg agacggggca
     4561 acggtcacca cggagaacgg catgaagcac tcgattacat tggcatcgct cacgattggc
     4621 ctgctcggca ccgcttgcgg agggtacacc aagataggag agggcagcgg cggcagcacc
     4681 ggaactccaa ccggcggggc cgcgggcggc aacggaggca gaagctatgg cggcacggcc
     4741 aatcgaggcg gtgcagcgcc gacgggcgga acctcgagtg cgcgaggtgg ttccactttc
     4801 ggtggcagta cttcgcgcgc aggcgagtca ggaatcggca acgcgacggg gaccgggaac
     4861 acgacggggg ccggcggcgc gaccggcatg agctgcaaca ccgatagaga ctgtccccga
     4921 agcttggttg cttgccaact ctgcgccgac ggcacgaccg cctgcccttg ggctgtctgc
     4981 gaaaacggca cgtgcaggag cggcatcgat cgatgcccgc cccccagccc ctgcgccggg
     5041 aaagcctgcg gagagctctg cacgacgtgc agcgacgaac agccgtgccc gcccgtgctc
     5101 atgttctgtg acgaacaagg gcaatgccag cgggacgagc ccacgtgcaa agctccactt
     5161 tgcaagacgg atacagactg tccggaagta gaggcgtgtc cgccgtgttc ggacggaaac
     5221 tgcgccgagt acaagtgcgt gagtggcgca tgccaattcg cctgtgaatc gcagggagga
     5281 agcggcggcg caggcggggg cacgtgctcc gctcaaggcg cgagttgcgc gaacggggag
     5341 acctgctgcg ccgggctcga gtgttgtgca ggcgtccccg ttcccgaggg taatgagtac
     5401 tgcggcacgg tctgccccaa gagcgacgtg aacatcaagc gagacttcct ctccgtagat
     5461 ccgaaccaga ttctggacaa gctcgcgagt cttccggtgg gcacctgggc ctaccgcacc
     5521 gagggctcga ccgagcgtca catcggaccc atggcgcagg acttcaaggc tgcatttcag
     5581 gtcggctcga gtgaccgcac gatcttgcag gtcgacgcgg acggcgtcgc gttcgcggcg
     5641 attcaggcgc tgaacgcgcg tttgaagacg ctcgaagagc agaatcgaaa gctgcgcagc
     5701 gacttggacg gcgtccggca acgttgtgca ccataaccga tcactccgct agctcgaggc
     5761 cctggaccac cacgggatcg ctccgatact gagtggttcc gtccccgagc tgaccgcgac
     5821 tattatctcc ccaacatacg acgcttcgat cggagcgcag ggcgcacgtg tgctgggcac
     5881 cggcggcgac ttgaaccgcc tcggacacga acgcgattcg ggtaggcttc gagtgaggta
     5941 gcgcgtctcc aagttgtcct gacccgttca ttccccaaca ataaatacta tggtccgccg
     6001 tcaaggcaca ggtgtgcgac tccgaggtag agagctgcac gacattggtg agttccggga
     6061 ttggcgtcgg cacgggccgg tcctgattcg tgccgtcacc gagctggcca aattcgtttg
     6121 ctccccagca ctgcgtcccg ccgtccgaaa gcagcgcgca cgcgtgaccg gagcctaccg
     6181 ctatctgtcg cacgcgagag agcgatacca caggcatggg aaggcccggg cttagagacg
     6241 ttccgttgcc gagctgacca cgctcgttgt ctccccaaca ccgagcggtg ccgtccgtaa
     6301 gcactgcaca agtttgtagg ctgctcgccg cgagactcac tacgttcgac aagctcggta
     6361 cctcgatggg cgtgttcgtg ttggctgaag tgctgtcccc gagttgacct ctccagttgc
     6421 ttccccaaca agaaacggag ccgttctgga gcagtgcgca ggcgtgttcg ctgccgagcg
     6481 cgagttgcgt ggcatcgccg agcattacaa cgggcagcgg ggctaaggac gcttcgacgc
     6541 tcacgcccgc cccgagttgg ccggagttgt tgagtcccca gcagtaggcg cggtaatctg
     6601 agttcaaggc gcagccgaag gggcctccca ggctgacttg ctcgacttgg tggatgagga
     6661 tcatatctcg gggtgaggct gagtactgtg tggcgtctcc agtgccgagc gccccaaaat
     6721 cgttctgtcc ccagcatctc acggacttgt ccgcgagccg tgcgcaggtg ctgtttccgc
     6781 tggtagcgag atcggtgact gccgagaccg gcgtcggtgc gcctggcccg ccggtgccgg
     6841 atagctccgt cgtcagcact cgtcgggtgc tcgtgtaagc cactcgacgg cccaccgccg
     6901 cgctcgggcc tggacttcta tcattcagtc ggaaggaaac cttgccatcc gagaggtcgt
     6961 aaactgctcg ctcggaacgc acgtaaatac gctcgccatc gatcacatcg tagggtcttt
     7021 cgagaggagg gaaccacgtt ccgcgtccaa tcgccgggat cgataccggg gtggaggatg
     7081 tggcctcggc gttcgttagg atgagtccgc cgcttcggcc gtaaaccagc aaacgctcat
     7141 catcgaccca agttgccggc gacccctccg ttacgccgat gagcatcccg ttgcgataga
     7201 ttaacgtatt ggtagtctta gcgccctgcg gatcgacggg gcccgtcggg ctgcccaccg
     7261 cgagttgagc gttgttgggc gagattgaga ggggcaggat gaattcatag gccgagtcct
     7321 ctgcgaagta agtttcgctt ccgtccaggc tcgtcagcat atgccggcag tgcgccgtgt
     7381 aacccacgcc actcaacgga tccttctcat agcggcaatt ctgctgcgcc agtcgggttc
     7441 cgacggggga tagcgcgaag tcgtaggcgg gtgtgggccc attgggatct tcgtacttcc
     7501 atgaactaat cgtgcgcatg gacggcaacg agaacacgct cagttctcga tcccccagat
     7561 actggtcgcc aaagcgcgtg ccgagcgccg ccacgacttg accgtcctgc gagagctcca
     7621 gccgacccgt agggtaagcc aaggcgcgct cgaacgtggg gtggctctcg acgtcgaagg
     7681 agaggagttg ccccgccgcg gtggcgaaga cgaccctgcc cgcgctcgag ccggccatgc
     7741 tgagtacggc tccgcagctg agcggtcttg gcgcgagggg ttgattcaaa cggccgccat
     7801 tcaggaccgc tccgccagca gttccggcga tccaacggcc gctcgcgtcg gacgcaaaac
     7861 tcgaaacgta ggctgcgggc agcgagtacg gtacctgctc gacactcgat ctcgtgagat
     7921 cgaggacact gaagggcgca tccgcccacg ggaccagccc gaggctgccc tcactcggca
     7981 cgacgtgata ggtagacccg gtcggaggga acgtggctac cggttctgca attcccaagc
     8041 gataaatctt gagtggctcc gccgagctga agtcctcgta cgtccagaag taatcaccat
     8101 aaccggcgag atttccgaca ctcgacagga tggtgagcgg atgcatgagg ttcgcatcct
     8161 ggtcgtacac gcgaaccacc gagccgacgc tagtcaggaa cttcgtcccg tccgcgaacc
     8221 aggagtgaaa ctcgcctaag tacggcgagc tcgagctcga ctgacccgtt ggtacggaga
     8281 tgagctcgat aacgttgcta gcgacagggc ctttcgcaac gcggattccc tgtggggcag
     8341 ctacgagtcg cgcgtcgagg tagttgcccg aatgctcgaa caagacggtg ccggatagag
     8401 aaatgaccgt gagcgccttt gaagaacgcg tccagagata gctgccgtca atggcgagcc
     8461 caacctcgac ggacctcggc acgaaccctc cccattcgga cggaagaatc tctggttctg
     8521 gtttggtgat agaagaaatc aggctcgcat caagtgccga gcgcacttcg agagtgcgtt
     8581 gcttcgggac cagcaacgtg tctcccgcca aaccaaaggc gcagtctttc ccacgaagcg
     8641 ggcaatcgcg ttgagtgatg tctcccgacg cgatgcgcac gtgactggcc aagtcccaga
     8701 gcagccaatg ccctgttgaa tcaagcgcca gtccacgatt tgcggacacg cgagccagct
     8761 cgatgccagc ggtgtgccca aggtcgatga gggtgtccgg gcccaaatcg acaatgccgc
     8821 agggctgcgc ggagattgtc ggttctccac ccgtgccgga agcgccgccc gtggttgtgt
     8881 tgccggcggt accgcccgaa gcagtgccgc catccgcttc ggtcccgccc gcgtcaccac
     8941 cactgcctcc gaacgtgctg tcgccaccgc tcgtcgagct cccgccggca ctagctcccc
     9001 ccgagctgtt gccggccgaa atcccaccgg agccgctcgt gacacccgtg ctcgtcccgg
     9061 tctgcccgcc tgtttcacgt ccccccgaac tcggcaccgc gcctgctccc gtttggccag
     9121 cgccgctcat cccggcgaac tctccgcttg cgtgcagctc caaactgcac cccaggcagc
     9181 ttcccaggca acccacgaac cagcccaagc cacgagcgcc cctcgcgaac accctgaaat
     9241 gaagctgcct catgctgtcg tcctcctcgg caaatcagtg agtgtccgac gagcgtgcga
     9301 ttcgatgaac ccagacgaac gaatcaaagc gcacatacgc gcaccggcgg agcccgaccc
     9361 cgttgaacct gtcgcctcta aacttgggtg cgcccttctc gcgcacccaa gagtcaggtg
     9421 cgattcacgg ctgagagctt tggctgttga cggtattcac gccgagcccg gccaaccact
     9481 cgccgacacc gagaaaagaa cccaatgatg tgcgagtgtg ccaatcggtc catgaaacgc
     9541 cgccgaggtt cgaatcgtcc tccggcggct tgaaggtgta atcggagttg acgccgacga
     9601 ctcgagggtc gccgttattc ggcagataga gggggcctcc cgaatctcca agacacaaac
     9661 tcgcattttg agggtcttgg tcatgtccgg cggtgatcac gtacgaggcg tccaccatcg
     9721 cgacctgctc gtcggagatc cggctatcga aacgacggat ttcagaagca gaaatggcgc
     9781 gcgcgttggc agccttgaac cgtcctagct tcacggtgct cggatcgaca ttggttcgct
     9841 cttcacagcc ccagcccacc tttaccaacg gagtatccac cccaacgatc ccaagttcaa
     9901 cgcgagcttg tggaatcgcg ggggtatctt cggccacctc aatcaccgcg atatcagcgg
     9961 cgccatcctg tgagccgagg atgatgtcct ggcaccgcgg gcaggaccac caagagggat
    10021 gaatggtgg
//
LOCUS       DASTKU010000640         7313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1909514, whole genome shotgun sequence.
ACCESSION   DASTKU010000640 DASTKU010000000
VERSION     DASTKU010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909514"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(464..922)
                     /locus_tag="VFQ61_22420"
     CDS             complement(464..922)
                     /locus_tag="VFQ61_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957277.1"
                     /translation="MTRVPEEQVSRSAELEQSELVDSPAATNADGSAAVEVAGIPLAV
                     HGNGPIGAGFQQQIHSRLSSTLKGARVERVIVRFEDLNGPKGGIDTACRIQVVLSGEP
                     DLVVEARAEGEAHAFRLAVPRLTAALKHRLEKRGGRVHGKSLRAMPVKDS"
     gene            complement(1570..2931)
                     /locus_tag="VFQ61_22425"
     CDS             complement(1570..2931)
                     /locus_tag="VFQ61_22425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957278.1"
                     /translation="MRKFLPFLRLSGPCFLLAWACTPLRDLDRAGAGEDKSVGGGVTT
                     LKGGEASSGASAGNAGVAGTGSPSGGSGNSGTSGVTSTGASHSGASTGGAGSGSAPQN
                     LAGGGAQAGGQASFGGASPSTTQYATGGAPGGNANLGGQTEGGTGTTTSGGAASSGGA
                     ASGGASMPAGAPSGGASGGRGGSSGGGSAGTLPNLQDIADSPVSGQITLSQEAEWEVD
                     AGKMAPTFEIKTPTASYWLVKAAAGFSSIEDPNKKQWIAYSYGYRPTRGLPDLGVCCQ
                     PGDPSKLMLPSVTTVLDEESFSKTHARLISKSTSGDIHIVWDFFLTHVTITIQKLNKI
                     PYGFTYSGVIGTALDSTDRLGLPTGVSRDANSAWNDDLPGPAEWVYFSDPDHGQSLFL
                     IQHADDALPDSYQVAYQDTGKFVFGAGQSTTLPIRFSMGIVGSTLHADVAKRVQFVID
                     QVK"
     gene            complement(2999..3943)
                     /locus_tag="VFQ61_22430"
     CDS             complement(2999..3943)
                     /locus_tag="VFQ61_22430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957279.1"
                     /translation="MSQAGTARGIALGLALTIIAQVSAARAEDKRACTAAYEEGQRLK
                     QSGELSAAREKLLICGGAGCPSVMHADCERWLSEVEASLPTVVFEVESERDGALNAAS
                     VAIDGRAPVTLQGRALSFDPGEHEVRFLAEGHEPAVRHYFFSEGQKLRRESVTLRVLT
                     SDGGGPSSEPPATSGESETTGSEPHSIAPVSSGDSRGSRFGVPFIIASSVAVAGAGGF
                     VFFGLDARSRDRALGACEPNCAHGDIQATRDRYLYANVALGVGVASAVTAVILYLVKP
                     GKSAASTRSRGSIASRPWPSGQSVSGFSEFGSSALLRF"
     gene            complement(3940..5547)
                     /locus_tag="VFQ61_22435"
     CDS             complement(3940..5547)
                     /locus_tag="VFQ61_22435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957280.1"
                     /translation="MTNSPVPVSPLKKSTVRPSIARLEKSTSEAPVTEQQIERIGRYT
                     LEALIAVGGMASVYLGSSTGAADFTRVVAIKRMHPHFAQDAEFVSRFRDEAWLCARLL
                     HPNIVQTFDVVEWVHELLLIMEYVEGVTLHALRTDANLSATQFPVPVALGVVVPALHG
                     LHAAHEAKDDDGHPLRLVHRDFTPHNLLVSREGHAKVLDFGIAKARGQLHATSTGQLS
                     GKFGYLSPEQIHADAVDRRADIFAAGIMLWETLTGERLFREQGLSDAGVLDRVLRKPI
                     PAPSSINPEVSVGLDAIALKALARDPTARVDTARDFALALEAELAPASPSRVSEFVLQ
                     TSGARLARLAELHAGVRRRPNLPPRRLPAALEPADPTIAVLPELTRSRIQSRPRKRAL
                     WLIPVTLLAAPLALYSVDRFRGVQRPAPPDLAVSAGGAPKSSGGSVRAPTTPPHAAIQ
                     PEPPAIASATPVVNDQPAVVGSGAQKAQSNAPNSRVKVATAEAKRASSKASASKSSAP
                     KPMMDDRCNPPTYVDAKGIRHFKEGCL"
     gene            5983..6249
                     /locus_tag="VFQ61_22440"
     CDS             5983..6249
                     /locus_tag="VFQ61_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HET9957281.1"
                     /translation="MCRNIRPLFNFEPPATEEEIRAAALQYVRKVSGTRQPSGKNVEV
                     FANAVERIAEVTRYLVDSLETHATPKNREDEAEKARERNKKRFG"
     gene            complement(6371..>7313)
                     /locus_tag="VFQ61_22445"
     CDS             complement(6371..>7313)
                     /locus_tag="VFQ61_22445"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9957282.1"
                     /translation="LGESCDDGNTVSGDGCSATCQVETACGNGLVEGVEQCDDRNTVS
                     GDGCSNVCQLEICGNNVTDPAEQCDDGNTVSGDGCSNACRLESACGNGKKEGAEQCDD
                     GNNRSGDGCSSACRIEGCGDGTTQALAGEQCDDGNTRDRDGCSSTCKLETKCGNGLVE
                     GVETCDDGNTVSGDGCSASCRLEVCGDGTVQGKEACDDGNTRSGDGCSATCSKEVRCG
                     DGEVNQSSEACDDGNNVSGDGCSSTCKEEVCGDGITQASRGEQCDDGNTASGDGCRPD
                     CQRELCGDGKVDKGEQCDDGNNVAGDGCSPFCQSEIR"
BASE COUNT         1323 a         2458 c         2198 g         1334 t
ORIGIN      
        1 taaggagcag gcatcgactt caaaaaatca tcgaactgct cttcggcttg gtgggcttgg
       61 tcattcatcc gagtcagctt cgcttgttgc actggcttcg cttctgcatg gatcagcttc
      121 gctgccgttg catctctgca gccatcccaa actgtgctgc ttctcggtga cgatgtcgag
      181 ccccggtcgt catccgcagg ggttcatcat tcccgaggtc ccaccttgcg cagcgcgcag
      241 aactccaccc accgcgcatc caatcaggtg cgccgcgtaa acaggagagc cgaaccgcgg
      301 aagctaccgg tcgggtactc gctgaagcta cacctgcgga aggccgttcc acgatgccgc
      361 ccctagacgc ccccgagcct cgatttcgcg ctgcaacgcc cccatcagcg cgaaaccaag
      421 gcttcggtgc tcgcgggatt ccgcgagcgc aacgcggagc gaatcagctg tccttgacgg
      481 gcatggcccg caacgactta ccgtggacgc gcccgccccg cttctcgagg cgatgcttca
      541 gtgccgcagt caatcgcggc actgcgagcc ggaatgcatg agcttcacct tccgctcgag
      601 cttccaccac caaatctggc tcgccgctca acaccacctg aatgcggcac gccgtatcga
      661 tgccgccctt gggaccattg aggtcctcga accggacgat cacgcgctcc acgcgcgctc
      721 ccttgagggt actcgagaga cgggaatgaa tctgctgctg aaagcctgcc ccaatcgggc
      781 cattgccatg aacagcaagc ggtatgccag ccacctccac agcggcgctc ccgtcggcat
      841 tcgtcgccgc ggggctgtct acgagttcgg actgttcgag ttctgcggac cgcgaaacct
      901 gctcttccgg gactctcgtc atacttggac aacataccac actaggtctc attcccgttt
      961 cgcctgatat tcgtcacgtc cttttggaac attccccgcg ccttcggtct gtttcgcgga
     1021 ccggtcctct gctgttggcg gctcttacga acttcgatat gtcgccctga ttaggcgttt
     1081 tcgcggtaat tctcgcggcc ggcggccgaa cacctacatt ctcgagtgcc ccaagagctc
     1141 gggagccagc cgacatgccg aatctggata cccaatctcg gatgccgtcc tggtgtggaa
     1201 tgcgcggagc taacgggtca accccgccgc ccgcgcgcgc caaccccgaa acctgaggag
     1261 ccatcgggag ccacgcacga accctcccct ccccattgcc gccgggctgc caccttacgg
     1321 gcgcaacggc actgcccccc tcgacggact ccaacttcat caccgagtgg ccggcgcgat
     1381 cgcgtttagg aggtcaagcc accgatagct ggggtttggc caccaaacca cgagcgcgac
     1441 cgatcgtcgg cctcccccac cagcgcagcc caattcgccc acgagccaat ccccaccgcg
     1501 cgggccctcc tcaatgcgat gtgctcgatt cagaagctcc gcttgccgac ggcaccccgc
     1561 tggctgtctc tacttgacct gatcgatcac gaactgcacg cgcttcgcga cgtctgcgtg
     1621 cagggtcgag ccgacgatac ccatactgaa tcgaattggg agcgtcgtac tctgcccagc
     1681 gccgaacacg aacttgccgg tgtcctgata agcgacttga taggaatcgg gcagggcgtc
     1741 gtccgcatgt tggatcagga acaatgactg gccgtgatcg ggatcgctga aatagaccca
     1801 ctcggcgggc cccggcaaat catcgttcca agcgctgttc gcgtctctgg acaccccggt
     1861 tggcaaaccg agtcgatccg tcgaatcgag cgcagtccca atcacacccg agtaggtgaa
     1921 accgtagggg atcttgttga gtttttgaat cgtaatcgtg acgtgagtga ggaagaaatc
     1981 ccacacgatg tggatatctc cagaggtaga cttggagatc agacgtgcgt gcgtcttact
     2041 gaagctctcc tcatccagca ccgtcgtcac gctgggcagc atgagcttgc tgggatcccc
     2101 gggctggcaa caaacaccca gatcgggaag ccctcgcgta ggccgataac cgtagctgta
     2161 cgcaatccat tgcttcttgt tggggtcctc gatcgaggag aagccggccg ccgctttgac
     2221 gagccaatag ctagccgtcg gcgtcttgat ctcgaaggta ggagccattt tcccggcgtc
     2281 cacttcccat tcggcttcct gcgagagcgt gatttggccg ctcacggggc tgtccgcgat
     2341 gtcttgcaag ttgggtagtg tgccggctga accgccgccg gaggagccgc cacggcctcc
     2401 gctggcaccg ccactgggtg cgcccgcggg catggacgcg ccgccgcttg cggcacctcc
     2461 gctcgatgcg gcgccaccgc tggtcgtggt tccagtgcct ccttcggttt gcccgcctaa
     2521 gtttgcgttg ccgccgggag cacctccggt cgcatactgg gtggtggaag ggcttgcgcc
     2581 gccaaacgag gcctgacccc ccgcttgcgc gccgccaccg gccaagtttt gcggcgcgct
     2641 ccccgaaccc gctccgcccg tggaagcgcc gctatggctc gccccggtac tcgtgacgcc
     2701 gctcgtgccg gagttaccac tgccgcccga cggtgacccg gtgccggcca ctccggcatt
     2761 tcccgcgctg gcgccggacg acgcttcacc acccttcaga gtagtgactc cgcctccgac
     2821 gctcttatcc tcgcctgcgc ccgcccgatc caaatcgcgg agcggcgtgc acgcccacgc
     2881 caacaagaag cacggccccg aaaggcgtaa aaaagggagg aattttcgca ttgccaattt
     2941 ccgcagacgg ttgggaccga gctctattca aagcccaaac aactcgaagc caagcagatc
     3001 aaaacctaag caatgccgac gagccgaact ccgaaaaccc actcacggat tgccccgagg
     3061 gccaaggccg actggcaatt gaccctcggc ttcgagtaga agcggcagat ttccctggtt
     3121 tcaccaggta caagatgacg gcggttaccg cgctggcgac acccacgcca agcgcaacat
     3181 tggcatacag ataacgatcg cgggttgctt ggatatcccc atgagcgcag ttgggttcac
     3241 atgcgccgag cgcgcggtcc cgactccgcg cgtccaggcc gaagaacacg aagccgcccg
     3301 cccctgccac tgccaccgaa ctcgcgataa tgaagggcac cccgaagcgg ctgccccggc
     3361 tatccccgga gctcaccggc gcgatcgagt gcggctcgct acccgtggtc tctgactcgc
     3421 ccgaggtggc gggcggttcc gagctcgggc ccccgccatc cgacgtcaga acgcggaggg
     3481 tcacactctc tcggcgtagc ttctgccctt ccgaaaagaa gtagtggcgc acggcgggtt
     3541 cgtgcccctc ggcgaggaag cggacctcgt gctctcccgg atcgaagctc agggcccggc
     3601 cttgtaacgt gactggcgcg cgtccatcga tcgcgacgct cgccgcgttc aacgcgccgt
     3661 cccgctcgct ctcgacctcg aacacgaccg tgggcagaga ggcttccacc tcggacagcc
     3721 agcgctcaca gtcggcgtgc atcacgctcg ggcaaccggc tccgccgcag atgagcagct
     3781 tctctcgagc agcgctgagc tcaccgcttt gcttcagccg ctgcccttct tcataagcgg
     3841 ccgtacaggc ccgcttgtcc tcggcgcgag ccgcgctcac ctgcgcgatg atcgtcaacg
     3901 caagcccgag cgcgatccct cgcgcggttc ccgcttggct cataagcagc cctccttgaa
     3961 gtggcggatc cctttcgcat ccacgtacgt gggcgggtta cagcggtcgt ccatcattgg
     4021 cttcggcgct gagctctttg aggctgaagc cttggaagac gcacgttttg cttccgccgt
     4081 ggccactttg actcggctat tcggcgcgtt gctttgggcc ttctgagcgc cggagcccac
     4141 gacggcgggt tggtcgttta caactggcgt cgcgctcgcg atggccggtg gctcaggctg
     4201 aatcgcggca tggggtggcg tcgtgggcgc acgcacggaa cctccgctgg atttcggcgc
     4261 accccccgcg gacacggcga ggtccggtgg cgcgggtctc tgtacaccgc ggaaacgatc
     4321 gaccgaatag agcgccaagg gcgcggcgag cagcgtcacc gggatcagcc aaagggccct
     4381 cttgcgtggt cgcgattgaa ttcgactgcg cgtcagctcg ggcaggaccg caatggttgg
     4441 atcggccggc tcgagagcgg caggaagccg acgcggcgga aggtttggcc gacgccgcac
     4501 gcccgcgtgc aactcggcca ggcgcgcgag ccgcgccccc gaggtctgca gcacgaactc
     4561 ggacacccgc gacgggctgg caggtgcaag ttcggcctcc agcgccaacg cgaagtctcg
     4621 cgcggtatcg acgcgagcgg tcgggtcgcg cgccaaagct ttgagcgcaa ttgcgtccaa
     4681 gcccacgctc acttcgggat tgatcgaact tggcgcagga atgggcttac gcagtacgcg
     4741 atccaacacg cccgcatccg acaggccttg ctcgcggaag agtcgttccc ccgtcagcgt
     4801 ctcccaaagc atgatcccgg cggcgaagat atccgcacgt cgatccacgg cgtcagcgtg
     4861 gatctgctcg ggtgagagat agccgaattt gccggagagc tgccccgtcg aggtagcgtg
     4921 cagttggccg cgcgccttcg cgatgccgaa gtcgagaacc ttggcgtgcc cttctcggct
     4981 aaccaataag ttgtggggag tgaaatcgcg atgaacgagc cgcagcgggt ggccgtcgtc
     5041 atccttggcc tcgtgtgcgg cgtgcaagcc atgcaacgcg ggcaccacga cgccgagcgc
     5101 aacgggtacc ggaaactgcg ttgcggacaa gttggcgtcc gttcgcaagg cgtgcagagt
     5161 tactccctcc acgtactcca tgatcagcag gagttcgtgc acccactcca cgacatcgaa
     5221 ggtctgcacg atgttggggt gaagcagccg agcacacaac caagcttcat ctcggaaccg
     5281 cgaaacgaac tccgcatctt gggcgaagtg cggatgcatg cgtttgatcg cgaccacacg
     5341 cgtgaagtcg gccgcccccg tagatgagcc caagtaaacc gaggccatgc cgccgacagc
     5401 aatcaaggct tccagcgtat agcgaccgat ccgctcgatc tgctgctcag tgaccggagc
     5461 ttccgaagtg gacttctcga gccgagcgat cgaaggacgc accgtgctct tcttcagcgg
     5521 cgagaccgga acgggggagt tcgtcatgcg ccgagcccat tccgaagctg attcacgcca
     5581 cgcaagcggc gctccccgaa agttcgtaca caggtagaac tagcgacatt gccttcatga
     5641 acgctgactg atgaaccctg actgcaacaa tgggcccagg ctagcattga agtcgaatgg
     5701 ctcacgcacg cggctgatga caactgtgac ggttgatggg gccgtggaat gcggcgggcg
     5761 aagggctcga atagacggcc gggaaaaaag gcaaacgcag tggactgtgc ggcggagccg
     5821 acccgacact tggcgagcga gccggctcaa aaccttggcg gtggagccac ccaatacaca
     5881 gtggcctggc gtggcgccag cagtccacct gcatcgttaa tcaccccaac ctacataggc
     5941 caactggccg caatatctcg ttcgggttag gttgttcgct caatgtgccg aaacattcgc
     6001 cctcttttca atttcgagcc ccccgccacg gaagaagaga tccgggccgc agccctgcaa
     6061 tacgtaagaa aagtgagcgg cacacgccaa cctagcggga agaacgtcga ggtgttcgcg
     6121 aacgcggtcg aacgcattgc cgaagttacg cgatatctgg tcgactcgct cgagacccac
     6181 gccaccccaa agaaccgcga ggacgaagcg gaaaaggctc gggagcggaa caagaagcgc
     6241 tttggatagg cgcccgaacg atcgccccac cgtgtaaccg cgagcctctg ttggttcgct
     6301 ctcggtgcgc gggctcagga ggggggcgcc gaggcacgtc gagacgtgcc tcggctttca
     6361 cttccggtcg ttagcggatc tcgctctggc agaacgggga gcagccgtcg ccggcgacgt
     6421 tgttgccgtc gtcgcactgc tcgcccttgt ccaccttgcc gtcgccgcat agctcgcgct
     6481 ggcaatccgg acggcagccg tcgccggacg cggtgttgcc gtcgtcgcac tgctcaccgc
     6541 gtgaagcctg agtgatgccg tcaccacaga cttcttcctt gcaggtggag ctgcagccgt
     6601 cgcccgagac gttgttgccg tcgtcgcagg cttcactgct ttggttgacc tcgccgtcgc
     6661 cgcagcgaac ctctttcgag caggtcgcgc tgcaaccgtc accgctgcgg gtgttgccgt
     6721 cgtcacaggc ttctttgccc tggacggtgc cgtcgccgca gacctcgagt cggcagctgg
     6781 cgctgcagcc gtcgcccgac acggtgttgc cgtcgtcgca ggtctccacg ccttcgacca
     6841 agccgttacc gcacttcgtc tcgagcttgc aggtgctgga gcagccgtcg cgatcgcggg
     6901 tgttgccgtc gtcgcattgc tcgccggcca acgcctgcgt cgtgccgtcc ccgcaacctt
     6961 cgattcggca cgcggagctg cagccgtcac cagagcggtt gttgccgtcg tcacactgct
     7021 cggcaccttc cttcttgccg ttgccacagg ctgactcgag gcggcacgcg ttcgagcagc
     7081 cgtcgccgct gacggtgttg ccatcgtcgc attgttcggc cgggtcggtg acattgttgc
     7141 cgcagatctc gagctgacac acgttggagc acccgtcacc gctcacggtg ttccggtcat
     7201 cgcattgctc gacgccttcc acgaggccgt tgccgcacgc ggtctccacc tggcaagtcg
     7261 cgctgcagcc gtcgccgctg acggtattgc cgtcatcgca cgattcgccg agg
//
LOCUS       DASTKU010000641         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1909660, whole genome shotgun sequence.
ACCESSION   DASTKU010000641 DASTKU010000000
VERSION     DASTKU010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1909660"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFQ61_22450"
     CDS             complement(<1..632)
                     /locus_tag="VFQ61_22450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9957283.1"
                     /translation="MTFTVALAACNPARGSSGRGGLHRWGEAVPGPSVSAEVPVRATD
                     RGPIRIEPLPGIDPPVFAHHGRPRGPGKLVFLHGMCGHGMGYAQAFQFAAARYGTLIA
                     PQGDSRCGNGPWAKWSRDLEALDRRILEGFRALGLAEPIEDVIVIGYSMGATRALELA
                     RKYPARYSSVILMGAPAAPTPGAASGLRAAVMMAGEHDRQDQMRAGVRAF"
     gene            798..2306
                     /locus_tag="VFQ61_22455"
     CDS             798..2306
                     /locus_tag="VFQ61_22455"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HET9957284.1"
                     /translation="MAKAQKLLQEEDWKRYAKWQSAGYFELVTDDTGTVPVRLFFTEQ
                     LLADAELSLYRQIVHATRFPGTRLVAVTPDAHHGYGVPVGCVIVTDREQGAVAMGPVG
                     FDIGCGMMSAKSEVPVEAATPDRRLAFNREVMDRVSLGTGGQSIRFGTLPEREFEELV
                     RGGAEYYVQKYGAEFDRSRAERHRIPVDDTWEIPWGGPGTPERGLDQLGSLGGGNHFV
                     ELQSCSETGTLFVQVHTGSRGFGHGLATNYFQMARDERPGEISDLDLGYFTPESSHFS
                     AYLNAVAAGGNYAILNRLVIYEQIARAFRKVFKSDLELVYEISHNLVQAEHHPEYGDV
                     WVHRKGATRAFPSGHPALRGTIWENAGHPVLIPGSNRDHSYVLRPDARAVNSAYSVNH
                     GAGRRMSRGEALRQLDQHKVNDQYRRDGIVVNLDGEVPLDESGQCYKDAAEVVAAVVS
                     AGLARIEYTLSPLASLKGNERGAARDRRRDKSKRERSRDQERDAARRRKGHY"
     gene            2428..>3630
                     /locus_tag="VFQ61_22460"
     CDS             2428..>3630
                     /locus_tag="VFQ61_22460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957285.1"
                     /translation="MLSSARRVAAGSPPEQPASQLDQRRRHSSLELILAFAILAVLGF
                     GGLAARELVGSSAERRPHQRSQSNPSLVAPARAPAFSIGVSPGAPVPDETSAKLSALP
                     STPTSALEFSAAPLSIPFHAPHAEAQAESELLRGPQSAAFTAKGITQTPKHAGSPNMA
                     ERVLWKHRGLCGDDDSDPNNPRNRYLREFGQLPASWSKGLFAYPNAPAEVIEQVRREV
                     PSLPSRVQAFLGLTSEPPAVYVHRDPQALRDYSCVNATAVAYYDGNMHVSLSGGIRFR
                     QELTISLEHEYVHHALMSHGVGQPIWFQEGAAMVFAQENWEDYRFTTQLIPLESMSRA
                     FAHTAKPEGARAFYLQAYAMVRLLRAVWRDPARSSEHALLDALLAQQATPEGLFEWAL
                     EQRMGHLER"
BASE COUNT          710 a         1175 c         1140 g          605 t
ORIGIN      
        1 tggaacgcgc gcactcccgc acgcatctga tcctgacggt catgctctcc cgccatcatc
       61 accgccgcac gcagaccaga cgcggcgccc ggcgtcggag cggcaggagc gcccatcagg
      121 atgacactcg agtagcgagc agggtacttg cgcgccaact cgagcgcccg agtggcaccc
      181 atcgaatagc caatcacgat tacgtcttcg atcggctccg ccagcccgag cgctcgaaag
      241 ccttcgagga tgcgtcggtc gagcgcctcg agatctcgcg accacttcgc ccatgggcca
      301 ttgccacacc gcgagtcgcc ctgcggcgcg atcagagtgc cataacgagc ggccgcgaac
      361 tgaaaggctt gcgcgtatcc catgccgtga ccgcacatgc cgtgcagaaa gaccagcttg
      421 ccgggcccgc gcggtcgccc gtgatgagcg aacactggag gatcgatgcc gggcaacggt
      481 tcaatacgga tgggacctcg atcggtcgcg cgcaccggga cttcggccga caccgatggg
      541 cccggcacag cctcgcccca acgatgcaat ccgccgcgcc ccgagctgcc acgcgcagga
      601 ttgcaggcag ccagtgctac ggtgaaggtc acgagaagcg ctgaagcaac cctcgcagat
      661 gcagaaagca tacgaggagg ggagcaaatt ccaggcctgc cgagcagacg gcgcgcagaa
      721 gggctaatat gtcgcgagcg actgctcctt gcgggaggct aactccactc ccgggtgcgt
      781 tcgagaaact tcgaatcatg gccaaagctc agaagctcct acaagaagaa gattggaagc
      841 gttacgcaaa gtggcagtca gcaggctact tcgagctcgt cacggacgac acgggcacgg
      901 tgcccgtccg gctcttcttc accgagcaac tactggcgga tgccgagctc tccctgtacc
      961 ggcaaatcgt acacgccacg cgatttccgg gcacccgcct cgtcgccgtg acgcccgatg
     1021 cgcaccatgg ctacggcgtg cccgtcggct gcgtgattgt caccgaccgc gagcagggag
     1081 cggtcgcgat ggggccggta ggcttcgata ttggctgcgg aatgatgagc gccaagagcg
     1141 aagtaccggt cgaggccgcc acgcccgatc ggaggctcgc gttcaaccgg gaagtaatgg
     1201 accgcgtgag cttgggcacc ggcggccaaa gcattcgctt cggcacgctg ccagaacgtg
     1261 agttcgagga gctcgtgcgc ggcggcgccg aatactacgt gcagaagtac ggcgccgagt
     1321 tcgatcgcag ccgcgccgaa cgtcaccgca ttcccgtcga cgacacctgg gaaatcccgt
     1381 ggggtgggcc cgggactccc gagcgcggcc tggaccaact aggttcactc ggcggaggga
     1441 atcacttcgt cgaactccag agctgcagcg agacgggcac cttgttcgtg caggtgcaca
     1501 cgggcagccg cggcttcggt catgggctgg ccaccaacta ctttcaaatg gcgcgcgacg
     1561 agcggcccgg tgaaatcagc gatttggatc tcggctattt cactccggag tcgagccact
     1621 tcagcgctta cctcaacgcg gtggcggcag gcggcaatta cgcgatcttg aatcgtttgg
     1681 tgatatacga gcaaatcgcg cgcgccttcc gtaaggtttt caagagcgat ctcgagctgg
     1741 tctacgaaat cagccacaac ttggtgcaag ccgagcacca ccccgagtac ggcgatgttt
     1801 gggtgcaccg caagggcgcc acgcgcgctt ttccaagtgg gcaccccgct ctgcgtggaa
     1861 cgatctggga gaacgccggt catcccgtac tgattcccgg ttccaaccgc gaccacagct
     1921 acgtgctgcg gccagatgcc cgcgccgtga atagcgcgta cagcgtcaat cacggcgccg
     1981 gacggcgcat gtcacgggga gaggccctgc gacagctcga tcagcataaa gtgaacgatc
     2041 aatatcgacg tgacggcatc gtcgtcaatt tggacggcga ggtcccactc gatgaatctg
     2101 gccaatgtta caaagacgcg gccgaggtgg tggcagccgt ggtttccgcg ggattagccc
     2161 gcatcgaata tacgttgtct ccgctggctt cgttgaaagg taacgagcgc ggcgcggcgc
     2221 gcgatcggag gcgcgacaaa tccaagcgtg agcgctcgcg tgaccaggag cgagacgccg
     2281 cccgacgccg aaagggacac tactagtttg cacgcaggcg cgtaactgag cgttagcgct
     2341 ggcgcgcggt gctgcgccgt gtaaagtccg accgtggcgc ggcagttcac ttcgcctcgg
     2401 cttcgaccgg ctgagcctcg ataccctatg ttgtcctcgg cccgtcgggt cgcggcagga
     2461 agcccacccg agcagccagc ctcgcaactc gaccaacggc ggcggcactc tagcctcgag
     2521 ctgatcttgg ccttcgccat tcttgccgtg ctggggttcg gagggctggc agctcgagag
     2581 ctcgtgggct cgagcgcgga gcgccgcccg catcagcgat cgcaatccaa tccgagtttg
     2641 gtggccccgg cgcgtgcccc tgcgttctcc atcggtgttt ctcccggcgc gccggttccg
     2701 gacgagacga gcgccaagct ctcggcactg cccagtacgc caaccagtgc cctggagttc
     2761 agcgcggcgc ccctatcgat tccgttccat gccccccacg ccgaggccca agccgaatcg
     2821 gagctgctgc gcggcccgca gagtgcagcc ttcacggcga agggaatcac tcaaacccca
     2881 aagcacgccg gctctccgaa catggccgaa agggtactct ggaagcaccg cgggttgtgc
     2941 ggagacgatg attctgaccc gaacaacccg cgtaaccgct acttgagaga gtttggtcaa
     3001 cttcccgcga gttggtccaa ggggctcttc gcttacccca atgcacccgc cgaggtgatc
     3061 gagcaggtac gccgtgaggt gccgagtctg ccgagccggg tccaggcctt cttgggtttg
     3121 acgagcgagc cgcccgcggt ctacgtgcac agggatccac aagcgctgcg cgattactcc
     3181 tgtgtcaacg caacggccgt ggcctactac gacggcaata tgcacgttag cctgagtggc
     3241 ggtatccgct ttcgtcagga gctcacgatt agtttggaac acgagtacgt gcaccacgcg
     3301 ctcatgagtc acggcgtggg ccagccgatc tggtttcaag agggggccgc catggttttc
     3361 gcgcaagaga actgggagga ctatcgcttc accacgcagt tgattcccct cgaaagcatg
     3421 agccgcgcct tcgcccacac ggccaaacct gaaggcgcac gcgccttcta ccttcaggcc
     3481 tatgcgatgg tgcgcctcct gcgcgccgtc tggcgggacc ctgcacgcag tagcgaacat
     3541 gcgctgctgg acgcgctgct cgcgcagcaa gcaacgccag aagggctatt cgaatgggcg
     3601 ctcgaacaac gcatgggcca tctcgaacga
//
LOCUS       DASTKU010000642         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_124005, whole genome shotgun sequence.
ACCESSION   DASTKU010000642 DASTKU010000000
VERSION     DASTKU010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124005"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFQ61_22465"
     CDS             <1..747
                     /locus_tag="VFQ61_22465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957286.1"
                     /translation="SSEAIARLLPAITAAARAASIHDPALRLLYASTYTAFRKRRSLL
                     LLNLERQTKLGDLPWIRAIQPWVGGDSASAAASREVLCDAAVLTLSAFPQTIVPNKLV
                     KELRALAAASGSPLPFVEELAADIFMGTFSETYLKAAQLAAGLLRSTLYERYYGIPYD
                     RILTLNDIQKVRRGDLAWICHSLAAACVQCRAIGRARELRCGQWHGDRASADSDDPQP
                     RGPVCGDWCGRGFAIAASGAGPSVFRMDLP"
     gene            788..1060
                     /locus_tag="VFQ61_22470"
     CDS             788..1060
                     /locus_tag="VFQ61_22470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957287.1"
                     /translation="MQKNTAYAWRQLVFYLSLLSASEQAEFLAWSTTHLAEQSLDFRS
                     RFEPVLSGLRLTLAGETFDSNGRHASGARRFLGWSIGRHWLMPKEA"
     gene            complement(1077..1982)
                     /locus_tag="VFQ61_22475"
     CDS             complement(1077..1982)
                     /locus_tag="VFQ61_22475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9957288.1"
                     /translation="MPVNLDDVRVFAAVARAGGFRELSRLSGMSASSASEAVRRLEAE
                     VGVRLLNRTTRTVTPTDAGVRLLERVGPALAEIASALDGVKGSSDQPAGTLRLNVPTV
                     AARIVLPQILPAFLSAYPEIRVEVTAEDGLVDIVAAGFDAGIRYEETLEKDMIAVPIG
                     PREQCYALAASPAYLEKHGRPNHPRKLLDHACLRVRFSRGALVTWEFERDGELLKIDP
                     AGPLVATLGNASEVLISAALAGSGIIYFFEDWLRPHFESGALKPILERWWLRFSGPFL
                     YYPSRRHMPAPLRAFVDFVRAQRKT"
BASE COUNT          378 a          667 c          629 g          388 t
ORIGIN      
        1 tcgagcgagg caattgctcg cctcttgccc gcaatcaccg cggccgcgcg agccgcatcg
       61 attcatgacc cagcgttacg cctcctctat gccagcacct acacggcctt ccgcaagcgg
      121 cgctcgctgc tgctgctcaa cctcgaaagg cagaccaaac tcggcgacct gccgtggatt
      181 cgagcgattc agccctgggt aggcggcgac tcagcatcag cggcggcttc ccgtgaggtg
      241 ctgtgcgacg cggcagtgct tacgctgagc gccttcccac agaccatcgt accgaacaag
      301 ttggtgaaag aactccgcgc gctcgcggcc gcatccggct ctcctttgcc tttcgtcgag
      361 gagctcgcgg cagacatctt catgggtacg ttttccgaga cctacctgaa agccgcccag
      421 ctggctgccg gcttgctccg cagcacgctt tatgaacgct actacggcat cccatacgac
      481 cggattttga ctctaaacga tatccagaaa gtccggcgcg gcgatctcgc ctggatttgc
      541 cactctctgg cagcagcttg cgttcaatgc cgcgcaatcg gccgcgcccg ggagcttcgt
      601 tgcggtcaat ggcacggtga tcgagcaagc gcagattctg acgacccaca acctcgcggc
      661 cctgtttgcg gggactggtg cggccgaggc tttgcaatcg cggcttccgg cgctggcccg
      721 agcgtgtttc gtatggatct gccgtagaca acaggtcacg cgcacggcct ggcaagtccg
      781 gctccggatg cagaagaaca ctgcctatgc ttggagacag ctggtgtttt atctgtcact
      841 gctttcagcc tctgagcagg cggagttctt ggcgtggagc actactcacc tagcggaaca
      901 gtcgttggat ttccgctctc gcttcgagcc agtcctgagt ggattacgcc taacgctggc
      961 cggcgaaacc ttcgactcga atggtcgcca cgcctccgga gcccgccgct ttttgggttg
     1021 gagcattggc aggcactggc tgatgcccaa ggaggcttga gccttcgcac ccaacgtcat
     1081 gtcttgcgct gcgcgcgaac gaagtcgacg aacgcgcgca gcggggccgg catgtgacgg
     1141 cggctcgggt agtaaagaaa gggaccggag aaacgcagcc accaacgttc gagaattggc
     1201 ttcaaggcgc cactctcgaa gtgcggccgg agccagtctt cgaagaagta aatgatgccg
     1261 ctgccagcga gcgcggcgga aatcagaacc tctgatgcgt tgccgagggt agcgacgagc
     1321 gggcccgccg gatcgatctt caagagctct ccgtcgcgct cgaattccca tgtgacgagg
     1381 gcgccacgcg agaagcgcac gcgcaaacag gcatgatcga gcagcttgcg cggatggttt
     1441 ggacgcccgt gtttttcgag ataggccggc gacgccgcga gcgcgtagca ttgctcgcgc
     1501 gggccgatgg ggacggcaat catgtccttc tcgagtgtct cttcgtagcg gatcccggca
     1561 tcgaagcccg ccgcaacgat gtctaccagc ccgtcctccg ccgtaacttc gacccggatc
     1621 tctggatatg ctgacagaaa ggccggcaag atctgcggaa gcacgatgcg cgccgcgacc
     1681 gtcggaacgt tgagtcgcag cgtgcccgcc ggttgatcgc tagagccctt taccccgtcg
     1741 agggcagacg cgatctcagc gagcgcgggc cccacccgct cgaggaggcg cacgcccgcg
     1801 tcggtcgggg tgaccgtgcg cgtggtgcgg ttgaggagtc gaaccccgac ctcggcctcg
     1861 agtcgtcgca ccgcttcact ggcgctcgac gcgctcatgc cgctcaggcg actcaactcc
     1921 cggaaaccgc ccgcacgcgc caccgctgca aagactcgca cgtcatcgag gttgaccggc
     1981 attgttcgct tttccgtacg gcccgtacat attcggagcc cttatcgtac acgggattag
     2041 gctcatacta gccctcatga ac
//
LOCUS       DASTKU010000643         7177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1015507, whole genome shotgun sequence.
ACCESSION   DASTKU010000643 DASTKU010000000
VERSION     DASTKU010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015507"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1205..3004
                     /locus_tag="VFQ61_22480"
     CDS             1205..3004
                     /locus_tag="VFQ61_22480"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957289.1"
                     /translation="MTELRGESNDSLRPGAKFLKYHIVERIGRGDRSSVYSGYDPFMS
                     RHVAIKILSRPGGVTREMLQRSQGEAKLLSRLRHKNIVEVYDAGVTDSGFPYIVMELL
                     RGRTLRAALEEHGKLAIEEVLHLFAQIADGLAKAHEEGAIHRDLKPENLFLSEGNCPK
                     ILDFGLAKIADGAAFATQPNVVHGTLLYMSPEQLQGALSTPRSDIYSLGTTLFEALYG
                     KPPCLLANPHPTIKELALAQAQLLAPRLDRLDSNIPEYVAKMVSRALAKLPDERQSSM
                     TELAAQLRDCQVRYLEDVERSQHRLSTRELSRPRATRLEAKRLAKLAGPAAAETPAKD
                     VAAGVGKNSTQGVSFPTRYVTLGAILLGGFVGSVLAATHLGASSAQLPPRTPPSAHLA
                     AATPGELETHSSVGSALAPRADLPAASADAAAAPEAPPNPHPEPELEIVEGPTPGHDS
                     AATSSVSGVSANSNTSAALSPALATDHARAAEPKATTEAFARRAQLTEARAPAEAHWS
                     GKAAQSPSTPSSPAQAAAVTPPHAAPPAKPAAVGATASQAAKRARDDQARESQARRDL
                     LDQALQWFERDLKKSDSTGERSSSSARVPKEAR"
     gene            3001..5004
                     /locus_tag="VFQ61_22485"
     CDS             3001..5004
                     /locus_tag="VFQ61_22485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957290.1"
                     /translation="MTTKPDSQGQNRAAVACLNDPFPNPGASSQHAERTASAGTDALL
                     FASDDDEPTLSDVITPLPGTLRQVNPLEGLEGSEEPSDDPLGLDDAVTRVTSIGAELA
                     KAAELEQPPSREALPRPALERDVVETTHVRPAAEAKPRTPPPKPERRSGTSASRRITP
                     RIDLRELREQTKSEVGPEPAKSEPARQESRSEPKSSRFKTPPPPKPLRALESRRQSDS
                     ESRMAAASSSSASGAFRLENGQIVSSVPATPSSAPGNPSAGSPDVQRSHLAVVSGAVV
                     NNNDRVEERPSSRNRSSRPAPDFELSTPDRPFNPYRLSTFVLPEGLRGQLNALEPPSL
                     SDADFEDRETTPPPAPREPSEITQVAPWAEATTPAAADALMGRQVTPPALPVQPESRP
                     PASSLNSPSVQPFTYPSVPAGSSSLLITTPSANRSNPANVAHREARARRFVGLAAAAF
                     GLSLLGFGAVTYLQSTDWLSDNGSQGSGSHELGDAPAEPLLEAGNAAPESELRVLAAS
                     PAAPAAAVKEAKPADTPREPAASSSAKAPIEERASAPAPERVAAPAASPSVAIISKPP
                     PLAAAGGWQVQSAGSTKPKAPVVVSAVAPATKAAPLAAVPPTKTAALPAKPKTEPVQV
                     LPAKPAAKPIAPREKAPTVAAASPSKGRSKSSVFDTMMAPPEE"
     gene            5063..6121
                     /locus_tag="VFQ61_22490"
     CDS             5063..6121
                     /locus_tag="VFQ61_22490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957291.1"
                     /translation="MSRPAFALFLGFSLLTAAPVRAESKANSEFGGKSKAENATAQTQ
                     YRKAYQLMRERKWPAARQILSELWAQAQTYDVAASLSQVDFQLGAFASSAEYMSFALS
                     HVPPKEKVETVQRYRTALAELKTKVGTVTILVNRPDAEVSIDGRVIGQSPLSADVFVD
                     PGARSFAAQLAQGLTQQELNVEPGKSYRVELDFPEQDPSLAQDASLRSPRAVTTGFSG
                     AEAAFVDRRRNFTPAIVTGAVAGAGLISGLVLLVAANKKSEDADSIRESLGGSNPCGV
                     GTIYDRDCSNLRDLSDDAATLRAVSYASFGAALAAGVVTYVLWPKAKRPPSMGLAMIP
                     GPAVAGGASVQAFMNGAF"
     gene            6140..>7177
                     /locus_tag="VFQ61_22495"
     CDS             6140..>7177
                     /locus_tag="VFQ61_22495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957292.1"
                     /translation="MWARLRGAMLLWMLSTPLAAALSCEGPCDEKGTCGEYDGPLGGT
                     GGRNDSTSGGVTQPESGSGGSPSSGGAIAGGSITGGGEAGDSGEGGTGPKTDGGAAGQ
                     AGMAGDAGNGGVGGESDACQGACKPPTAICTDGGECVECLKPEDCAQVTGRPLCDTDK
                     GACVACLADKDCGSAATSHCVAGICAKCTKDEECTHITGKGLCTAAGCAQCSVQNEKP
                     CGDFSCNPKTLSCTKTTRASVRTCGACLADSECKADHACVPMKFGTTQRPGGYCLQIK
                     TKYCPPPYYVPLSGVASLSGVPNGTYCGIDTDETTCEAVVDMEQSAACGPPTMSVEPS
                     DSLCACRRDAQG"
BASE COUNT         1387 a         2259 c         2291 g         1240 t
ORIGIN      
        1 gtcatgtatc gtttggactt ggtgatgccg gccgcggacg gcctttcttt gactaattcc
       61 tggctgcggg agcgtgagct tgtcaccaac tcgaaagttc ccggtcagga gcttgcaggt
      121 aattcgagct cgagttgcaa aaagatagtc gcaactcgag ccgaaagaat gaatcgccat
      181 tcgaggttct aggagagcgg cgacggtaac gaatacccgc cggtgggacg cgcgcttggc
      241 tacgtctcgt gcagctcggc aatgggttcg cgcgtcgccc caggagtaaa gtaacgagcc
      301 gcaccggcgt ggagattcag tggtaggttc gagcggccag cgagcagccc tcggggccat
      361 agacctgtcg agtgtgcttg cccctggcgc aacacccggg ctcggcctcg acgttcttcg
      421 aactccagcc gttgctggcc cgctcgagac gagtctttgc ccatgaccgc aaacacagcc
      481 gaacgggtac gcagctggca tcagcaccgg cgtatcagca ccggcgtatc agtgaaggct
      541 taaaagcgaa gcagccgagc ggctaggtgt gcctctacta ctggcaaagc agttgttgtt
      601 tacggttcgc aggaacacgt cggttctggc ccggggcaga ttctggcgga gcggatgtgc
      661 aggtgggtaa tgcagggcca cgcgattgat gatcggcgct cgtcgatgaa acccccgttg
      721 attgtacaag ccaggccgaa tccaatgaac cacgcggtaa aaccgcgtgc tttcacagaa
      781 tcagagccgt cgatcgagac atctcgaacg agacctatcc cgagcgagac ccatcccggg
      841 agttggtaaa gcgtttcaga caacgcttcg agagcgcgtt gaagtgaagc gctctcggat
      901 gccttgcggc tctactcgtg cgcgagcagg caaagaaacc atgaaacaga aaccttgcgg
      961 aagtcggttc gtttctcggc cgtgaggcct gttatcgaac tgggtgggtg ccgaagctcg
     1021 ccgttgcgga gccgcgggat tcacggcatt aatcaagctc gagggcctgg gctcgaacga
     1081 gctcggtggc gcgtacgtgc gattccaaca tgtcctgctg gttctcacac tctagagctt
     1141 cacgttcgag cggagctcac tcaagccgac ggatcactga accgcgggta cccgaggtgc
     1201 tcggatgacg gaactccgag gggaaagtaa cgactcgctc cggccaggtg cgaagttctt
     1261 gaagtaccac atcgtcgagc gcatcggtcg cggtgatcgc tcgagtgtgt acagcggcta
     1321 cgacccattc atgagtcgtc acgtcgcgat caagatcttg tcgcgtccgg gcggggtgac
     1381 gcgcgagatg ttgcaacgca gtcagggcga agcgaagctc ctgagccggc tccgccacaa
     1441 gaacatcgtc gaggtatacg atgcgggcgt cactgactcg ggtttccctt acatcgtgat
     1501 ggagctgctg cgcgggcgta ccctgcgcgc cgcactcgaa gagcacggta agctcgccat
     1561 cgaagaggtg ctgcacttgt tcgcgcaaat cgcggacggg ttggcaaagg cgcacgaaga
     1621 aggcgcgatt catcgcgact tgaaacccga gaacctgttc ctctcggagg gcaattgccc
     1681 aaaaatcttg gatttcgggt tggccaagat cgcggatggt gcggcgtttg cgacgcagcc
     1741 caacgtggtg cacggcactc tgctctacat gagtccagag cagttgcagg gagctctgtc
     1801 tactcctagg agtgatatct attcgctagg cacgacgctg ttcgaagcgc tttatggcaa
     1861 gccgccctgt ctgctcgcca atccgcaccc gacgatcaag gaactcgcgc tggctcaagc
     1921 gcaacttctg gcgccccggc tcgatcgctt ggattccaac atccccgagt acgtcgccaa
     1981 gatggtcagc cgcgccctcg ccaagctgcc ggatgaacgt caatcgtcca tgaccgagct
     2041 cgcggcgcag ctgcgtgact gccaagtacg ttacctagaa gacgtcgagc gctcgcagca
     2101 tcggctgtct acgcgcgagt tgtcccggcc gcgggcgact cgtctcgagg ccaagcgcct
     2161 agccaagctg gcggggcccg cagccgcgga gacaccggcc aaagacgtgg ccgccggcgt
     2221 ggggaagaac agtacccaag gggtttcgtt ccccacgcgc tacgtgacgc tcggcgcgat
     2281 cctgctgggc ggtttcgtgg gcagcgtttt ggctgccact cacctcggcg cgtcttcggc
     2341 tcagctgccg cctcgaacgc cgcccagtgc acacttggcc gcggctactc cgggtgaact
     2401 cgagacgcac tcgtcggttg gttctgcgct tgcgcctcga gccgatttgc ccgcggcatc
     2461 agccgatgcg gcggcggctc cggaagcgcc accgaaccct caccccgaac ccgaactgga
     2521 gatcgtcgaa ggtcccacac cgggtcacga ttcggcagca acgagctcgg tgtcgggcgt
     2581 ctccgcaaac tcgaacacca gtgccgcgtt gagccccgct ttggctacgg atcatgctcg
     2641 cgcggccgag ccgaaagcca cgacggaagc gttcgcgcgc cgcgctcagc tgaccgaggc
     2701 tcgagcgcca gcagaggcac actggtccgg taaagcggcc cagtcccctt cgacaccgtc
     2761 atctcccgca caggctgcgg cggttacgcc gccacacgcg gcgccaccgg ccaaacctgc
     2821 agccgtgggc gcgaccgcca gccaagccgc gaaacgggcc cgcgacgacc aggctcgtga
     2881 gagccaggcc cgaagagatc tcttggatca agccctgcaa tggttcgagc gtgatctgaa
     2941 gaagtcggac tcgaccgggg agcgctcgtc gtcctcggcg agggtaccga aggaggcgcg
     3001 atgacgacca agcctgattc tcaaggccaa aaccgcgcgg cggtggcctg cctgaacgat
     3061 cccttcccca atccgggcgc ctcttcacaa cacgccgagc gtacggcgtc ggcaggaacc
     3121 gacgcgttgc tcttcgccag tgatgacgac gagcctactc tgagtgatgt gattacgccg
     3181 cttccgggga ctttgcgtca ggtcaaccca ctcgaggggc tggaaggttc cgaggagccg
     3241 tcggatgatc cgctcggcct cgacgacgcc gtaactcgcg tcacttcgat cggggcagag
     3301 ctcgccaagg ctgctgagct ggaacagccg ccttcgcgtg aggcgctgcc acgtcccgcc
     3361 ctggagcgag acgtcgtcga aaccacccac gtgcgccccg cggcggaagc caagccgcgc
     3421 actccacctc ccaagcccga gcgccgctct ggaacctcgg cgtcgcggcg catcaccccg
     3481 cgcatcgatc ttcgggagct tcgtgaacag accaagtccg aggtcggacc agagccggcg
     3541 aaatcggaac cggcccggca ggaatcgcgc tccgagccca agagctcccg cttcaagacg
     3601 ccgcctccgc ccaaaccatt gcgcgcgttg gagtcgcgtc gacaatcgga ctccgagtca
     3661 cgcatggcgg ccgcctcgtc gagtagcgcc agcggcgctt tccggctcga aaacggtcag
     3721 atcgtgtcga gtgttcccgc tacccccagc agcgcacctg gtaaccctag cgctggttcg
     3781 ccagacgttc agcgttcgca cttagcggta gttagtggcg cggtcgtcaa caacaacgat
     3841 cgagtcgagg agcgcccgag ctcacgaaat cgatcgagcc gcccggcacc cgacttcgag
     3901 ctcagtacac ccgacagacc cttcaatccg tatcggctct cgacgttcgt gttgccagaa
     3961 ggcttgcgcg gccagctcaa tgcgctcgag ccgccgagcc tctccgacgc ggacttcgaa
     4021 gatcgcgaga cgacaccacc ccccgcgcct cgtgaaccat ccgagatcac gcaggtggcg
     4081 ccctgggcgg aagcgacgac tccggctgcg gcggatgcgt tgatgggcag gcaggtgaca
     4141 ccccctgcgc ttccggttca gccggaatct agaccaccgg cgtcttctct caactcgccg
     4201 tcggttcagc catttacgta tccgtcagtt ccggctggct catcgtcatt gctgattacg
     4261 actccgagcg caaaccgctc gaatccggcg aatgtggcgc atcgcgaggc acgcgctcgt
     4321 cgcttcgtgg gtctagccgc ggccgctttc ggtctctctc ttcttgggtt cggcgcggtt
     4381 acgtatctgc aatccacgga ttggctgtcg gataacggga gccaaggcag cggcagtcat
     4441 gaactaggcg acgctcccgc agagccgctg ttggaggccg gcaacgcggc gcccgagtcg
     4501 gaactgcgag tgctcgccgc gagtccggcg gcacccgccg cggcagtcaa agaagcaaag
     4561 ccggctgata cacctcggga gcccgccgcc tcgagttcag cgaaagcgcc tatcgaagag
     4621 cgcgcctccg cacccgcacc ggagcgagtg gccgcgcccg cggcttcccc ctccgtggcc
     4681 atcataagta agccgccgcc actcgccgct gcgggcggat ggcaggtgca gagcgccggt
     4741 tcgaccaagc ccaaggcgcc cgtcgtggtc tcggccgtgg caccggcgac gaaggcagcg
     4801 ccgctcgcag cggtaccacc gaccaagact gccgctctac ctgcgaagcc gaagactgaa
     4861 cctgttcagg ttctgcccgc aaaaccagcc gccaagccta tcgcccctcg tgaaaaggca
     4921 cccacggtag cggccgcgag tccgtccaag ggccgcagca agagcagcgt ttttgacacg
     4981 atgatggctc ctccggaaga atgacagctc gggctatccc gggttacaac gctttacgga
     5041 agccatgaac ggaattggga caatgtcgag gccggcattt gcgctttttc tcggcttttc
     5101 tctgctgacc gccgctcccg tgcgcgcgga aagcaaagcg aactccgagt tcggcggcaa
     5161 atccaaagcc gagaatgcga ccgcgcaaac ccagtaccga aaagcttatc aactcatgcg
     5221 cgagaggaag tggcccgcag ctcgacaaat cctgtcggag ctgtgggctc aagcgcagac
     5281 ctacgatgtc gcggcttcgc tcagtcaggt cgacttccag ctcggagcat ttgcgtccag
     5341 cgcggagtac atgtccttcg cgctgtctca cgtgccgccc aaagaaaaag tggagaccgt
     5401 tcagcgctat cggacagcgc tggctgaact caaaaccaag gtcgggacgg tcacgatttt
     5461 ggtgaaccga cccgatgccg aggtctcgat cgacgggcgg gtaattggac agtcaccgct
     5521 gagcgccgac gtgttcgtgg acccgggcgc gcgctctttt gcggcgcagc tcgcgcaagg
     5581 tctcactcaa caagagttga atgtggagcc tgggaagtcg tatcgggtgg aactcgattt
     5641 cccggagcaa gaccccagct tggctcagga tgcgagtctc cgttcacctc gtgctgtcac
     5701 cacgggattc tctggtgcag aggctgcatt cgtggatcgc agacgcaact tcaccccggc
     5761 catcgtgacg ggtgcggtgg cgggagccgg tctcatctcc ggtttagttc tgctggtggc
     5821 ggccaacaag aagagtgaag acgcagattc gattcgggag agcctaggcg gcagcaatcc
     5881 atgcggcgtg ggaaccattt acgatcgcga ttgcagcaac ctccgcgact tgtcagacga
     5941 cgctgcgacg ttgcgagcgg tctcttatgc gagttttggc gcggccttgg ctgccggagt
     6001 cgtcacgtac gtgctttggc ccaaggcgaa gcgccctccc agcatgggcc ttgcaatgat
     6061 cccaggaccg gcggtggccg ggggcgcttc ggtgcaagcg ttcatgaacg gtgctttctg
     6121 aagaatagga tcgcgaatca tgtgggcacg attgcgcgga gccatgctgc tgtggatgct
     6181 ctcgacgccg ctcgcggcgg cgctgagctg tgagggccct tgcgacgaga agggcacctg
     6241 cggcgagtat gatggtccgc ttggcggcac cggtggaagg aacgacagca cgtcgggtgg
     6301 cgtaacccag ccggaaagtg ggtcaggcgg atcgccgtcg agtggcggcg ccatcgcggg
     6361 tggatccatc accggcggcg gggaggcagg cgactcgggc gagggtggaa ccggtcccaa
     6421 gaccgacggc ggggccgccg gacaggccgg catggccggt gacgccggca acggtggagt
     6481 tgggggcgag agcgacgcgt gccagggtgc atgcaagccg cccacggcca tctgcactga
     6541 tggaggggag tgcgtcgaat gcttgaagcc cgaggattgc gcacaagtca cgggtcgacc
     6601 gctgtgcgac acggacaagg gggcgtgcgt tgcgtgcctc gcggataagg actgcggctc
     6661 cgccgccacc tcccactgcg tggccggcat ttgcgcgaaa tgcaccaagg acgaagagtg
     6721 cacgcacatc acgggaaagg gactttgtac ggccgcgggc tgcgctcaat gcagcgttca
     6781 gaacgaaaag ccgtgcggcg atttctcctg caacccgaag acgctcagtt gcacgaagac
     6841 cactcgcgcc agcgttcgga cctgtggagc atgcttagcc gacagcgagt gcaaagctga
     6901 tcacgcttgc gtgccgatga agtttgggac cacgcagcgg ccaggcggct attgcttgca
     6961 gatcaaaaca aagtactgtc cgccgcctta ctacgttcct ctttcgggag tagcaagctt
     7021 gtcgggcgtg ccgaacggca cctactgtgg aatcgacacg gacgaaacaa cgtgcgaagc
     7081 agtcgtcgac atggagcaat ccgcggcgtg cggcccgccc accatgagcg ttgagccctc
     7141 agacagcttg tgtgcgtgcc gccgagatgc gcagggg
//
LOCUS       DASTKU010000644        10200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1686141, whole genome shotgun sequence.
ACCESSION   DASTKU010000644 DASTKU010000000
VERSION     DASTKU010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686141"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..2098
                     /locus_tag="VFQ61_22500"
     CDS             143..2098
                     /locus_tag="VFQ61_22500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957293.1"
                     /translation="MQPLRSVGLGLGGAGLGLIGLGLVAVTVRLGWASFIVGHPSASG
                     VVRSAALPPRIPKVLALSSDPRAAHRADIRSGSAQSAPAVDSARNPRRELEPSNPGRL
                     RNVNAFIPPQCYAKTSDSAGRVHNPCYVCHVSARAPNFVDDAQLQLEYAFEARARENP
                     WRNALENYAERSQRVSDAAILNYVRQSNYLDPRGEPLLGRLSREVPAEWDVDHDARWS
                     GFVPDARFVFDERGFDRTPDAGYTGWRSYGFYPLPGAFMPTNGAFGDALVRLPATFRE
                     TEEGTFDPQIYELNLALLESLITRRDVQIEPTDEARYGIDLDGNAKLGLANRAVFKGA
                     LASGVQFVGRAGAEQRSGSIQIVPGLYPVGTEFLHSLRYLDVGAAGVSMAPRMKELRY
                     ARKESFVPPAELARRAREEAEMEREFAALQGAAHHPPEAEDESLEEELGPRAVSGNAE
                     QGVRTDQGFRLQGFIEDRSGQLRPQSFEESVYCVGCHGGVGATDDGIFSFSRRLDYAS
                     PQHGWFHPTQHDLKGTRDPQRGGSSEYVRYLELNGAGDEYRANLEVLAKFFTGEGRLR
                     PEMRERLRRDVTELLLPSPERAYALNKAYYLVVQDQSFVFGRELAGGAERHVLRSVAP
                     GEKTRIARIEPAWRRTSPGVRAAPRGP"
     gene            complement(2144..2452)
                     /locus_tag="VFQ61_22505"
     CDS             complement(2144..2452)
                     /locus_tag="VFQ61_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9957294.1"
                     /translation="MNKRLAMLEKMTAASVADPFAFYALALEYRKEGRVQDALKTFET
                     LRSHHPEYLPMYLMAGQMLVSESRNAEARQWLESGIALAHQKGDTKTLGELQSELANA
                     "
     gene            complement(2454..4709)
                     /locus_tag="VFQ61_22510"
     CDS             complement(2454..4709)
                     /locus_tag="VFQ61_22510"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9957295.1"
                     /translation="MSELNEPQRLAVAHVSGPLIVFAGAGSGKTRTITYRIVNLLATE
                     HVPPYRILAVTFTNKAAAEMRQRVESLAGPEITRDLWIGTFHSICARLLRRYHAAVGL
                     SRNYVIYDDGDQKAVLSRVLKDLKLSEQLFSPKLVLSLISKEKRDGFYPEDSRAERRM
                     ESSLLDVYDRYQAALLSSDAVDFDDLLGFATRIAESKTPEGDELRSRFHHVLVDEFQD
                     TNLIQYRLVHALSRATRNLCVVGDDDQSIYRWRGADVRLIRNFRRDFADAQVIKLEQN
                     YRSTGNIVKAALGVIEPAHEREPKVLWTDSADGEKVRVRAVSDEREEATFVANSIRSE
                     LGRGLRPEQVAVFYRVHAQSRALEEALRGLNIAYQIIGGMKFFERAEVKDLLAYLRFI
                     TNPRSDMDLLRIINVPARGIGDKTVERVASAAAENTCSAYDALNRLLDHDELQGAARK
                     KLAGFRELIEELRDAARDLSPSKFAKHVLERTGYERLLKEEDSAEADARLGNLEELVG
                     ALQDYEKELEERDEQPTLEGYLERVSLIAGVDMLQDSKGVSLMTVHGAKGLEFDVVFL
                     TGMEETIFPYKGVDQTHGTSEEELDEERRLAYVAITRARKRLAITHASMRQLFGRTHY
                     LTPSRFLADLPERAIAREGSGLSAYGSAARFGAAARYSNTLRPYGGDDEPSFRAPPPA
                     LAPGTRFVERELDDSAGESVRVRPGSRVRHRHFGRGVVESVESGDSPVVVARFESVGK
                     KRIKSEFLEFD"
     gene            4835..5968
                     /locus_tag="VFQ61_22515"
     CDS             4835..5968
                     /locus_tag="VFQ61_22515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957296.1"
                     /translation="MQGHPSTKASAAPQLGTTPSSPAVTASAEPPSQDRGASTVYLVR
                     IPEKLLALPAECACCGGPTTQTQRLDLAQQRLLVGYCSICARHAFEFGRRKYSLVFAS
                     ALAGGATALGVPMGFPETPLWLCVLVAVVANGFFLALSALTPRPLSGHTAAGLAVTKA
                     DAFTLACARARFAERVALAAGSECIQVPAPSRFHALSPWLALLVLPVLLTIVSHDYHH
                     PRLRVLNLGGAPLRLVVDGGLAADVEPSSGESPKAGRELRVPAGPRHLQAFDSEGQLL
                     EETSVVLQSGREHLYVPGPSRACFWLETAGYGRDRAELRQQPLRQDRNFWILPAELRG
                     WFVPLPEAGEMTRFSGGSAMVLRQGDCALDMKPGPDPGLSGAD"
     gene            6192..6743
                     /gene="grpE"
                     /locus_tag="VFQ61_22520"
     CDS             6192..6743
                     /gene="grpE"
                     /locus_tag="VFQ61_22520"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET9957297.1"
                     /translation="MSDSNVTRDTQDPSNPAPESESNKANDAEIDPLEAARAETSKMR
                     EQLLRTAADFDNFRKRTSRETQDATRRAREDFLRDLLPIFDNLERAVAHAETATDVKS
                     LSDGVTMVMRMFADTLNKLGVERLTAVGLPFDPAVHEAVQQIETKDVAPGVVAAEIQA
                     GYRMGERLVRPAMVVVAKPPSSN"
     gene            6991..8817
                     /gene="dnaK"
                     /locus_tag="VFQ61_22525"
     CDS             6991..8817
                     /gene="dnaK"
                     /locus_tag="VFQ61_22525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET9957298.1"
                     /translation="MGKIIGIDLGTTNSCVSVLERTNAQGAPEVKVIANTEGARTTPS
                     VVAFTAKNERLVGQVAKRQATTNPESTVHAVKRLMGQRFDSAPVQQHARVAPYKVVRH
                     ENGDAWVEVQGKLFSPPEISSYVLGAMKQVAEAYLGESVSEAVITVPAYFDDAQRQAT
                     KDAGRIAGLEVKRILNEPTAAALAYGLDKKERETIAVYDLGGGTFDISILEIQNGVFS
                     VRATGGDTHLGGEDFDTRIIDKLAEAFLADNAIDLRKDRMSLQRLREAAEKAKHELSS
                     SLETEINVPFIASGPKGPVHLQRTLRRSELEIMTADLVERTISSCRSVLNDAKLGVEQ
                     IGSVVLVGGMTRMPAVQRAVRELFGKDAHKGVNPDEVVSVGAAIQAAALSGAIQEVLL
                     LDVTPLSIGVETGGGVFTRQIPRNTTVPTERSEIFTTSVDNQPFVPVHVLQGEREMAG
                     DNRSLAHFELTGIPPAPRGVPKIQVTFRIDANGILSVEAKDLGTGRTQAISVTATSGL
                     STEEIERLVKEGEKYQESDLLRRELAEVRNQAETLLYTTDQALEGYADLLEASQLDKV
                     RGEVSTLRKMLETGASLDALRDAYSRLEAATFELAEAMYGPS"
     gene            complement(8852..9691)
                     /locus_tag="VFQ61_22530"
     CDS             complement(8852..9691)
                     /locus_tag="VFQ61_22530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957299.1"
                     /translation="MRATTTAAGRRLAIIGLCCGLTAACGDRPPLFTMAAGSGGQGAS
                     GGQTNQGGTTAASGSTSPNGGFDPGTSSSGSTANGGITGQGGAAGHGGFNSKLQILNL
                     KVEDNRDDCLWVGDSDERLVYSDHEWHIEVGADEERARAGLRFEVKIPQGSRIESAIL
                     RLSRVSGTASAVDTMQVHVYDSSDVPEFADAHVHGPEGHVDAGLYAQPVRGMPAGLAN
                     GFTESPDLSPLIQHVVDRPDWEGEGHIGFVLSALSMQTWVGYADSTSERGAAELAIFY
                     VLP"
BASE COUNT         1809 a         3208 c         3299 g         1884 t
ORIGIN      
        1 gagggactcg aaaactgagc agaaaatacc agaattctga gtgaattagg ttatacgaag
       61 caggccggct tgcacgtcgt cattcgcacg tgctccgcag tttgctgaac ggtcatgctt
      121 gcgggctaca atcttcgccg cgatgcagcc gcttcgttcg gtgggcctgg ggctcggagg
      181 tgcgggtctg ggcttgattg gtttggggct agtcgcggtc accgtgcgcc tcgggtgggc
      241 cagtttcatt gtcggtcacc cctctgcatc gggcgtggtg cgctcggctg cgctcccacc
      301 acgcatccca aaggtgctcg cgttgagctc tgatcctcga gccgcgcacc gggcagacat
      361 acggtctggg tctgcgcaaa gcgcgcccgc tgtggactcg gctagaaacc ctcgccgcga
      421 gttggagcca agtaatccgg gccgcttgcg caacgtgaac gctttcattc ccccgcagtg
      481 ctacgcgaag acttcggatt ctgcaggccg ggtgcacaac ccttgttacg tctgtcacgt
      541 cagcgcgcgc gcaccgaact tcgtcgatga tgcgcagctg cagctcgaat acgctttcga
      601 agctcgcgcc cgcgagaatc cctggcgaaa tgctttggag aattacgcgg agcgctcgca
      661 gcgggtgagt gacgccgcga ttctgaacta tgtacgtcag agcaattatc tggaccctcg
      721 aggtgagccg ctgctcgggc gtttgagtcg agaggtgccc gccgagtggg acgtcgatca
      781 cgacgcgagg tggtcgggct tcgtgccgga cgctcgcttc gttttcgatg aacgcggctt
      841 cgatcggacc cccgacgcag gttacacggg ctggcgcagc tacggcttct atccgcttcc
      901 aggcgcgttc atgccgacga atggcgcgtt tggtgacgcg ctggtgcggt tgccggccac
      961 gtttcgtgag accgaggagg gaaccttcga cccacagatt tacgagctga acctcgcgct
     1021 gctggagtcc ttgatcacac gccgggacgt gcagatcgag ccgacggacg aagcgcgcta
     1081 cggcatcgac ctcgatggca acgccaagct gggtctcgcg aatcgcgccg tattcaaagg
     1141 ggcgttggca tccggagtcc agttcgtggg gcgagcgggt gcggagcagc gctcgggctc
     1201 gatccagatc gtgcctgggt tgtacccggt gggcaccgag ttcttgcact ccttgcgcta
     1261 tctcgatgta ggtgcggctg gcgtcagcat ggctccgcgc atgaaggagc tccgctacgc
     1321 tcggaaggag agcttcgttc cgccggcaga gcttgcacgc agggctcgcg aggaggcgga
     1381 gatggagcgt gaatttgcgg cgttgcaggg cgcggctcat cacccacccg aagcagagga
     1441 cgagagcctg gaagaagagc tcgggccgcg cgcagtatcg ggcaacgccg aacagggcgt
     1501 ccgaacggac caaggctttc ggttacaggg cttcatcgag gatcgttcgg gtcagttgcg
     1561 tccacagagc ttcgaggaga gcgtttactg tgtcggctgt cacggcggag ttggtgcgac
     1621 cgacgacggc attttttcgt tcagccgccg cctcgattac gcaagcccgc agcacggctg
     1681 gtttcatccc acgcagcacg atttgaaggg aacgcgcgac ccgcaacgcg gcgggagcag
     1741 cgaatacgtg cggtacttgg agttgaacgg cgccggggac gagtatcgcg cgaatctgga
     1801 ggtgctcgcg aagttcttta ccggtgaggg gcgcctgcgc cccgagatgc gcgagcggtt
     1861 gcggcgcgat gtgacggagc tgctcctgcc gagccccgag cgcgcgtacg ccttgaacaa
     1921 ggcttactac ctcgtggtcc aagatcagtc gttcgtgttc ggccgtgagc tcgcgggagg
     1981 tgccgagcgc cacgtgctgc gcagcgtggc gcccggggag aagacgcgca ttgcccggat
     2041 cgagcccgct tggcggcgga cctcacccgg cgtgcgtgcc gccccccgcg gtccatagcc
     2101 gcagccgcgt cgaccgcagg cggcgcgcgt gcttccgagc gactcaggcg ttggcgagct
     2161 cgctttgcag ctcgccgagg gtcttggtgt cgcccttttg atgagccagc gcgatgcccg
     2221 actcgagcca ttgccgcgcc tccgcgttgc gcgactccga aaccagcatt tgccccgcca
     2281 tcaagtacat cggcaagtac tccggatggt gtgaacgcag cgtctcgaag gtcttgagcg
     2341 cgtcttgcac gcgcccttct ttccggtact ccagagccag agcgtagaag gcgaacggat
     2401 cggcaacgct cgctgccgtc attttttcga gcatcgctag gcgtttgttc atttcaatcg
     2461 aactccagga attctgactt gatccgtttc ttgcctacgc tctcgaagcg cgcaacgacg
     2521 acggggctgt ctccggactc gaccgattcc accactccgc ggccgaagtg ccggtgtctt
     2581 acgcggctgc ccgggcgaac gcgcactgac tcacccgcgg aatcatccag ctcgcgctcg
     2641 acgaagcgag tacctggcgc cagcgcgggg ggaggcgcac gaaagctggg ctcgtcgtcg
     2701 cccccataag ggcgcaaagt attcgaatag cgcgctgcgg cgccaaaacg cgcagcgctg
     2761 ccgtaggcac tcaaaccgga cccttcgcgc gcgatggccc gctcgggaag atcggcgaga
     2821 aaccgagatg gagtcagata atgcgtgcgc ccgaacagct gccgcatcga agcgtgggtg
     2881 atcgcgagcc gcttccgcgc acgtgtgatg gcgacgtagg ccaggcggcg ctcttcgtcg
     2941 agctcctcct cggaggtgcc gtgcgtttga tctacgcctt tgtaagggaa gatcgtctcc
     3001 tccatgccgg tcaagaacac gacgtcgaac tcgagcccct tggcgccgtg cacggtcatc
     3061 aagctcaccc ccttggagtc ttgaagcatg tctacgccgg cgatcagcga gactcgctct
     3121 aggtaaccct cgagcgtcgg ctgctcgtct cgctcttcga gctctttctc gtagtcttgg
     3181 agcgcgccca cgagctcctc caagttcccg agccgcgcgt ccgcttccgc cgaatcctct
     3241 tccttgagca ggcgctcgta gcctgttcgc tccaatacgt gcttggcgaa cttggagggg
     3301 ctcaggtcgc gcgcggcgtc ccggagctcc tcgatcagct cgcggaaacc cgcgagcttc
     3361 ttgcgggctg cgccctgcag ttcgtcgtga tcgagcagtc gattcaaggc atcgtacgcg
     3421 ctgcaggtgt tctcggcggc ggccgaagcc acccgctcga ccgtcttgtc tccgatgccg
     3481 cgcgccggaa cgttgatgat tcggagcagg tccatgtcgc tgcgcgggtt ggtgatgaag
     3541 cgcaggtaag cgagcagatc cttgacctcg gcgcgctcga agaacttcat cccgccgatg
     3601 atctggtagg cgatgttcag gccgcggagc gcttcttcca gcgcgcggga ttgtgcatgc
     3661 actcggtaaa acaccgcaac ttgctccggg cgcagccctc tgccgagctc actgcggatc
     3721 gagttcgcca cgaaagtggc ttcttcgcgt tcgtcggaga cggcccgcac gcgaaccttc
     3781 tcgccgtccg cggagtccgt ccacaacacc ttcggctcgc gctcgtgtgc gggctcgatc
     3841 acgccgaggg cggccttcac gatgttgccg gtcgaacggt aattttgctc gagcttgatg
     3901 acctgcgcgt ccgcgaagtc ccgtcggaag ttcctaatca ggcgcacgtc ggcgccgcgc
     3961 caacggtaaa tcgactggtc gtcgtcgccc acgacgcaca ggttgcgggt ggctcggctc
     4021 agcgcgtgca cgagccgata ctgaatcagg ttcgtgtctt ggaattcatc caccagcacg
     4081 tgatggaagc ggctgcggag ctcgtctccc tccggggttt tgctctcggc gatgcgcgtc
     4141 gcaaaaccca ataggtcgtc gaagtccaca gcgtccgagc tcaggagcgc ggcctggtag
     4201 cgatcgtaga cgtccaacag ggaggattcc atgcgtcgct cggcgcgact gtcctccgga
     4261 tagaagccgt cgcgcttctc cttgctgatc aaggacagca cgagcttggg agagaagagc
     4321 tgctcgctca gcttgagatc cttgagcact cggctgagca cggccttctg atcgccgtcg
     4381 tcgtagatga cgtagtttcg gctgagcccg accgcggcat gatagcgtcg tagtagccgc
     4441 gcacaaatcg agtggaaggt gccgatccac aggtcgcgcg tgatttcggg gcccgccaag
     4501 gactcgacgc gctggcgcat ttcggcggcg gccttgttgg tgaacgtcac ggccaagatc
     4561 cggtacggcg gcacatgctc ggtcgcgagc aggttgacga tccgataggt aatagtccgt
     4621 gttttcccac tgcccgcgcc ggcgaacacg atgaggggcc cgctcacgtg agccactgcg
     4681 agtcgctgcg gttcgttcag ctccgacatg tgacggttcg gcctagaaca tcgcggccgc
     4741 gccggcaacg aaagccgtcg gatgcgaacc tcattcgtgg aagtgagcga gaaagcagca
     4801 gaacacaggc tgtgaactat actgcggccg gaacttgcaa ggtcacccca gcacgaaagc
     4861 cagcgccgca ccgcagctcg gcaccacgcc gagttcgccc gcggtcaccg cgtccgcaga
     4921 gccgccatcg caggaccgcg gcgcgagcac ggtctatctc gtccgaatcc ccgaaaagct
     4981 tctggcactc cccgcggaat gtgcgtgttg cggcgggccc acgacgcaga cgcagcgact
     5041 cgatttggcg cagcaacgct tactcgtggg ctactgttcg atctgtgcgc gccacgcgtt
     5101 cgagttcgga cggcgcaagt acagcttggt tttcgcgagc gcattggcgg gcggcgcgac
     5161 ggcgctaggc gtgccgatgg gctttccgga gacgccgctc tggctctgtg tactcgtggc
     5221 agtggtcgcg aacggcttct tcttggcgct ttcagccctt acgccgcgcc cgctttctgg
     5281 gcatacggcc gcgggcctcg ccgtgaccaa ggcggatgct ttcactctcg cgtgcgcgcg
     5341 cgctcgattc gcggaacgcg tcgcgctcgc tgccggctcc gaatgcatac aagttcccgc
     5401 tccgagtcgt tttcacgctc tgagcccgtg gctcgccctg ctcgtgctcc cagttctgct
     5461 cacgatcgtg tcgcacgatt accatcaccc aagactgcgg gtgctgaacc tgggtggggc
     5521 cccgctccgc ctcgtcgtag acgggggatt ggccgcggat gtggaacctt cgagcggaga
     5581 aagccccaag gcggggcgcg agctccgggt ccccgccggt ccgcgccact tgcaggcttt
     5641 cgattcggaa gggcagctgc tcgaagaaac atcggtggta ctccagtccg ggcgggaaca
     5701 cctgtacgtg ccgggcccca gccgcgcgtg tttttggcta gaaacagccg ggtacggtcg
     5761 cgaccgcgcc gagttgcgtc aacagcccct ccgccaggat cgcaactttt ggatcttgcc
     5821 cgcagagttg cgtgggtggt tcgttccatt acccgaagcc ggcgaaatga cccggtttag
     5881 cggcggctcc gcgatggtgc tccgccaagg agactgcgcg ctcgacatga agccgggccc
     5941 ggatccgggg ctttctggcg ccgattgaac caggccgttc gaatgccctg attctgcctc
     6001 tgaacgcatg cccgtccgag agccccgccc cgcggccgac gtctgttccg gtttcggcgg
     6061 tggggacggg ctctccgatg gggcggaata aaggagcggt aatggccgcg ccgcagccgg
     6121 cgaaaacggc cctcctcaag aagctcaatc cccgcgttgg caagctccga ggcgtggtta
     6181 tactgcgccg cgtgtcggac tcaaacgtga ctcgagatac gcaagacccc tcgaaccccg
     6241 cgcctgagag cgagagtaac aaggcgaatg acgccgagat cgacccgctc gaggctgccc
     6301 gcgccgagac ctcgaagatg cgcgagcagc tgttgcggac ggcggctgac ttcgacaact
     6361 tccgaaagcg aacctcccgc gaaacccagg acgcaacgcg ccgggcgcgt gaagatttcc
     6421 tgcgcgatct gctgccgatt ttcgacaacc tagagcgagc agtggcgcac gccgagaccg
     6481 cgacggacgt gaaatcgctg tcggacgggg tgaccatggt catgcgcatg ttcgccgata
     6541 ccctgaataa gctgggtgtg gagcgtctga ccgcggtcgg cctgccattc gatccggccg
     6601 tgcacgaggc cgttcaacag atcgaaacca aagacgtcgc gccgggtgtc gttgccgcgg
     6661 aaatccaagc tggatatcgc atgggggagc gtctggtgcg ccccgccatg gtcgtggttg
     6721 cgaagcctcc gagctcgaac tgaccagccc gcttacctta cggcggcgcg tgccctgaga
     6781 gcggccaact tagctgtcgg gctggtgagt ttagcgacaa tcgagcgcgc tcccgcggct
     6841 tgagttcgaa tcccgagcta gccggaacgc ggctcgtgtg ggcttcgctg ccccggcgaa
     6901 ctacctaaac cgtcccgagc gagttcagag ctctcgggca tgcgcgatcc aggctgacgt
     6961 gatccaagct gaaaggtatc ctggctgggc atggggaaaa tcatcggcat cgacctcggg
     7021 acgaccaact cttgcgtatc ggtgctggaa cgaacgaacg cgcaaggggc acccgaggtc
     7081 aaggtgatcg caaacacgga gggtgcgcgt accacgccct ccgtggtggc gttcacggcc
     7141 aagaacgagc gtttggtagg gcaagtggcc aaacgccagg ccaccaccaa tccggaaagt
     7201 accgtgcacg cggtgaagcg tctgatgggg cagcgcttcg attcggctcc ggttcagcaa
     7261 catgcgcgcg ttgcaccgta caaggtggtc cgtcacgaga acggtgatgc gtgggtagag
     7321 gtgcaaggca agctcttctc gccccccgag atctcgagct acgtgctcgg ggcgatgaag
     7381 caggttgccg aggcgtacct cggggaaagc gttagcgagg ccgtgatcac ggttcctgct
     7441 tacttcgacg atgctcagcg ccaggccacc aaagacgcag gtcgcatcgc cggtctcgag
     7501 gtcaagagga tcctgaacga gcccactgct gcggcactcg cttacggtct cgacaagaaa
     7561 gaacgagaaa ccatcgccgt ctacgatttg gggggtggca cgttcgatat ctccattctg
     7621 gagatccaga acggcgtgtt tagtgtgcgc gccacgggcg gcgacactca cctcggcggt
     7681 gaagacttcg atacgcggat catcgacaaa ctcgccgaag ctttcctcgc ggacaacgcc
     7741 atcgacctgc gcaaagatcg catgtcctta cagcgtctgc gtgaagcggc cgaaaaggcc
     7801 aagcacgagc tctcttcgtc gctcgagacc gagatcaacg tgccgttcat cgcttcgggc
     7861 cccaagggtc cggtgcatct acagcgaacg ctgcgacgca gcgagctcga gatcatgacg
     7921 gccgatctag tcgagcgcac gatctcttcg tgtcgctcgg tgctgaacga tgccaagctc
     7981 ggcgtcgagc aaattggttc cgtggtgctg gtcggcggca tgacgcgcat gccggcggtg
     8041 cagcgagcgg tgcgcgagtt gttcggcaag gacgcgcaca agggcgtcaa ccccgacgag
     8101 gtggtgtccg tgggcgcggc cattcaagcc gcggccttga gcggcgcgat tcaagaggtg
     8161 ctgctgctcg acgtcactcc actctcgatt ggtgtagaaa cgggcggcgg cgtgtttact
     8221 cggcagatcc cgcgcaacac caccgtcccc acggagcgca gcgagatctt caccactagt
     8281 gtggataacc aacccttcgt tcccgtgcac gtgcttcaag gcgagcgcga gatggcgggc
     8341 gacaaccgca gcttggccca cttcgagctg acgggcatcc cacccgcccc ccgcggcgtg
     8401 cccaagatcc aagtcacatt tcgcatcgat gccaacggta ttctatccgt ggaggccaaa
     8461 gacttgggaa cggggcgcac ccaggccatc agcgtgaccg ccacgagcgg gctctccacc
     8521 gaagagatcg agcgcctcgt caaggagggc gagaagtacc aggagtcgga cttgttgcgc
     8581 cgcgagctgg cggaggtgcg caatcaagcc gagacgctgc tgtacacgac cgatcaagct
     8641 ctcgagggtt acgccgactt gctcgaagcc tcgcagctgg acaaggtgcg cggtgaggtg
     8701 tcgacgctgc gcaagatgct cgagacgggt gccagtctgg acgcgctacg agacgcatac
     8761 tcgaggctcg aggccgcgac gttcgagctc gcggaagcga tgtacggacc gagctgacgt
     8821 cagttagagc tgcgtttcga tgcgctggag ttcagggcag cacgtagaat atcgccagct
     8881 cggccgcgcc gcgctccgac gtcgaatcgg cataccccac ccaggtttgc atcgagagag
     8941 cggagagcac gaacccgatg tgaccctcgc cttcccaatc gggtcgatcg accacgtgct
     9001 gaatcaacgg tgagaggtcg gggctctccg taaagccgtt cgccaagccc gcgggcatgc
     9061 cgcgcaccgg ttgcgcgtaa aggcctgcgt ctacgtgacc ttcgggaccg tgcacgtgag
     9121 cgtcggcgaa ctcgggcacg tcactcgagt cgtatacgtg cacttgcatg gtgtcgaccg
     9181 cgctggccgt gcccgacacg cgggacagcc ggaggatggc gctttcgatc cgggaacctt
     9241 gcgggatctt cacttcgaag cgtagccccg ctctggctcg ctcttcgtct gcgcccacct
     9301 cgatgtgcca ctcgtggtcc gagtagacga gccgctcgtc ggaatcgcca acccacaggc
     9361 aatcgtcccg attatcttcg actttgaggt tcaaaatttg gagcttcgaa ttgaacccac
     9421 catgccctgc cgcgccgccc tgtccggtaa tcccaccgtt cgctgtgctg ccactgctcg
     9481 atgttcccgg gtcgaaaccg ccattgggac tagtacttcc gctcgccgcc gtggttccgc
     9541 cctggttcgt ttggccaccg ctcgcgccct gcccgccgct gccagcggcc atcgtgaaca
     9601 gaggtggacg gtctccgcag gcggcggtga gcccgcagca caaaccaatg atcgcgagcc
     9661 ttctccccgc ggccgtggtt gtcgcgcgca acgaccgctt tgccgacgct ctgctgcgct
     9721 cagaaccgag gtcagggagc tgaccgtgcg cgagccgatg tggctgccgc agcatcgcag
     9781 ctaggcgaaa cggagagata ggggagggcc gacccacact acgagccatg ttcctaggag
     9841 tagcattctg cccatagttt ggggtggttc ggtagcattc aagtctggag taccaaccgt
     9901 gcgcccgtgc gctcgaagtc gcgcggcggg aacttcgagc gttcgagttc cgggccagac
     9961 ggaatgggct tcaacaatcg agtctgttgc gctgcgtacg ggcgaatggc tacctgcgac
    10021 ccctctgacg gaggacggac gggctgttcc tttggaaaag agcctcggag ttcaggaagt
    10081 gcgcgcaatc tgctcctgac cctgcggctc gtcgcttccg ccgcgatgct tcgcatccca
    10141 tcgtcaggcg caccccgagt tggcgagggg cggttataga agcgcaagct cgtactcggc
//
LOCUS       DASTKU010000645         7646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_346689, whole genome shotgun sequence.
ACCESSION   DASTKU010000645 DASTKU010000000
VERSION     DASTKU010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346689"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFQ61_22535"
     CDS             complement(<1..326)
                     /locus_tag="VFQ61_22535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957300.1"
                     /translation="MLALSRWIGVGAATGILLALVPVVRGSFGPLNEPLRKIMRPNVF
                     VEPPKPPDFSGLDLLRLELRPQRAIAPLTEGRMAELTIDPVLQRAARSQMQHYRIPEA
                     GTVMLDV"
     gene            complement(424..816)
                     /locus_tag="VFQ61_22540"
     CDS             complement(424..816)
                     /locus_tag="VFQ61_22540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9957301.1"
                     /translation="MPDRPARILVVDDSPIVLDATRDALEAGGYEVLTHCGEGSFEAV
                     LAEDPDLVLLDANIPHRSTRHLASLLASTGAAPPRQVLLYSSLSPPELARETKRVGAD
                     GYVEKTNDVTGLNRRVGAFLAARSKPGS"
     gene            908..1447
                     /locus_tag="VFQ61_22545"
     CDS             908..1447
                     /locus_tag="VFQ61_22545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957302.1"
                     /translation="MKPKAFWSCAACSLLLVTVAAGPACTSSCDKEDRTLVITSGRVT
                     TTRVDGSTTSSSVQELLGAAGADSGAESEAGVLLRVYESSPWGGPLARFEGGNTLEFE
                     HGLGVVPEWVSTYLSFAAEGTDKSNVAENAGDSGLIECVDAEIIRLRNNTCENEYSVR
                     VLAQATQQGSQSSQPCRTE"
     gene            complement(1481..2065)
                     /locus_tag="VFQ61_22550"
     CDS             complement(1481..2065)
                     /locus_tag="VFQ61_22550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957303.1"
                     /translation="MFERTITGVGLGLIMACQSATVAPAPPPAMPTASHGVEQFFPLK
                     DGIVYAYDTSSSSGDKGMLALEIRRTRSGAVELIVAGRTRRLELDDTGLRHATGGWLL
                     KLPLTEGATYPGDFGQVKVTSVDRKISVPLGNFAGCLETVEAVSNDQLSKRTTTVFCP
                     GVGIVQRQTDAETDTEAGSETLVLRSFGPRVQDL"
     gene            complement(2058..2567)
                     /locus_tag="VFQ61_22555"
     CDS             complement(2058..2567)
                     /locus_tag="VFQ61_22555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957304.1"
                     /translation="MSKASDTPPGTPSAPADGSLEPAPKPENSGSSATGDPGASGAAE
                     LGSSGTPERAEKRTKKRRSKNKALPEVAPRRANLDAKGRERPRFLLEFPEDPELERLI
                     GCFEAGNYRELRQGAPTLIERSTNPEIRAAAEELLRRIEPDPLMKLLLVAAIVLFICV
                     VAAAYGSHV"
     gene            complement(2567..4000)
                     /locus_tag="VFQ61_22560"
     CDS             complement(2567..4000)
                     /locus_tag="VFQ61_22560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HET9957305.1"
                     /translation="MPFNTLAFAKFFGVVFIVSWLLVRLRVARIVFLLLASYAFYAHW
                     DYRFLPLIFGSSSLDFWLARAIARAPDARHRKRWLWLTVVVNLSVLGFFKYANFGLDT
                     LSPLLTTFGVSRPTLEIALPIGISFFTFESMSYVIDVYRGDIEPHPSYLEYLTFVAFF
                     PHLVAGPIVRPRDLLPQLAAPGRFSNEAGAEGLFAIAIGLVKKIVIADFLAINLVDRV
                     FQAPLQYSSLECYAAAVGYALQIYCDFSGYTDIASGCALLLGVRFPRNFDAPYTAVNI
                     ADFWRRWHISLSTWLRDYLYIPLGGNRHGPTRTYVNLLLTMLLGGLWHGANWTFVAWG
                     GLHGAALALTRAYQDLPGSRKAATFGAQRSLSLPRRVLQTVLTFHLVLASWILFRSET
                     FTQARIIFGRIFQGTLYHPNLDARVLLVMLLGLTSHFVPDRWFAAAQAGFCRLPALAQ
                     AALLFCVSVLLRNVASAEAVPFVYFQF"
     gene            4158..5000
                     /locus_tag="VFQ61_22565"
     CDS             4158..5000
                     /locus_tag="VFQ61_22565"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9957306.1"
                     /translation="MLTPGASRLSPGAKPSPARVAFLLATVGVGALLVLALYRALVGQ
                     SSFAAWSTLGLAVLGWVVLSTFGVFFPWLAMYGPVVSRGPRGLNRVALTFDDGPHPVT
                     TRRVLELLRGTPHRATFFVLADKVARHPEVVREIFGDGHTIALHGAVHDRLHSFRGAN
                     RVERELRGARAELEALIGEQVRWFRPPLGHTSPATMRGVARVGLNVIGWSSRGYDGLE
                     RTSSREVLRRVLGTLNDGAIVLLHDAAERDDFEPKSLLILPELLAALDARGLRSVGLN
                     TWLK"
     gene            5115..6401
                     /locus_tag="VFQ61_22570"
     CDS             5115..6401
                     /locus_tag="VFQ61_22570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9957307.1"
                     /translation="MTDSTGSAPPRGMKTGGNDRDTSERPASGPAPRATQPPTTMAPR
                     RRRRWWVWGLAATSGLAVAVWAWPKGASSALAAEAPDVPRAEGKRVMFSDNYAKRIAL
                     ETVSVGTHDVVPAVSVVGTVDFDPEYVARVGTRLRGVVRSVSKFEGARVKRGDVLASI
                     DSPELGDAQASVASLRAEFNALARSRDREQALSARNLTTPREAEEATAQASRSQALLQ
                     AAEQRVSALAGRAASPKAQLGVHDVTSPLDGVVVERHVAKGQLVSSDHFAFLIANLDH
                     LWVELSVFERHLPGIKVGDEVELRSNSSAHAEPVKGRVARVGAVLEAESRSAQVRVEL
                     KNEQRRFVPGQSVVAVIRATAAEADDVPTVPHSAVVFVDGVPTVFVADSPTSVLVTEV
                     ELGESNGQVLHVKRGLSADQRVVTRGATALRNELFR"
     gene            6586..>7646
                     /locus_tag="VFQ61_22575"
     CDS             6586..>7646
                     /locus_tag="VFQ61_22575"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET9957308.1"
                     /translation="MLAAVVAWAIRARRAVLVGLVVLIACGIQAARSLPIDALPDVST
                     IQVSVLTTASGLSPLEVERTVTMPVEVALNGTPGGVELRSVSRSGLSAVTVVFKDGTD
                     VWFARQLVLERLRGIELPPAAAMPELAPVSTGLGEIYQFVVRSDQHSPMQLRTLLDWE
                     IVPKLRSVPGVVEVNTAGGELKQFQVDVDRVRLEARGLSLANVVDALRNANVSVGGGY
                     VERNKESFTVRGEGMLRDIQDIGKVIVKTSPNAAPVLIRHVADVHIGSALRYGVITQD
                     GKGEAVTGIVMMLLGANSKQVVTDVAERVKQIQKELPAGVRVEVIYDRADFVGRTLST
                     VMKNLLEGVAVVSVVLVLFL"
BASE COUNT         1395 a         2305 c         2596 g         1350 t
ORIGIN      
        1 acgtccagca tcaccgtgcc cgcctccggg atgcggtagt gctgcatctg gctgcgcgcc
       61 gcgcgctgca acaccgggtc gatggtgagc tctgccatgc ggccttcggt caacggcgca
      121 atggcgcgtt gcgggcgtag ctccaggcgc agcaagtcga gtccggagaa atccggaggc
      181 ttcggcggct cgacgaacac attgggccgc atgatcttgc gcaaaggctc gttcagcggg
      241 ccgaagctgc ctcggaccac aggcacgaga gccaatagaa tgcctgtcgc ggcaccaacg
      301 ccaatccaac gggagagcgc taacatacgg ttgccgacag cgagttagcg ggcctggcgc
      361 ggccgggcca acaaactggc ttcccgagtc ggtcgcgctc gagggctgaa agaattcagt
      421 cgctcacgaa ccgggcttgg agcgcgccgc caagaaggct ccgaccctgc ggttcaagcc
      481 ggttacgtcg ttggtcttct ccacgtaacc gtccgcgccc acgcgcttgg tctcgcgcgc
      541 tagctcggga gggctgaggc tcgaatacaa gagcacctgg cgcggtggcg cggctcccgt
      601 gcttgcgagt aggctcgcca agtgcctcgt cgatcggtgc ggaatgttgg cgtcgagcag
      661 gactaaatcg gggtcctccg cgagtaccgc ctcgaacgaa ccttcaccac agtgcgtgag
      721 cacctcgtag ccgcccgcct ccagtgcatc acgggtcgcg tccagcacga tcgggctgtc
      781 gtcgacgacg aggatcctgg ccggtcgatc gggcatcggg tttgctaacg ggcaatacgg
      841 ccacgctcat cgtgcccttc agcggaaacg cagcactgcg cggggagggg tgctagcgtg
      901 cgaccgcttg aaaccgaagg ctttctggag ctgtgcagcc tgcagcttgc tgctcgtcac
      961 tgtcgctgcg ggtcccgcgt gtacgtcgtc gtgtgacaag gaagaccgca cgctggtcat
     1021 cacctcgggc cgcgtgacga ccactcgcgt ggacggttca accacctcga gctcggtcca
     1081 ggagctgctc ggggccgccg gagcggattc cggagcggag tctgaggcag gcgttctgtt
     1141 gcgcgtctac gagtcgtcac cctggggcgg cccgctcgct cggttcgagg ggggaaacac
     1201 gctggagttc gaacatgggc tcggcgtggt gccagagtgg gtgagcacgt atctttcgtt
     1261 tgccgcagaa ggcactgaca aatcgaatgt ggccgagaac gcgggcgact cgggactgat
     1321 cgagtgtgtg gatgccgaga tcatccggct gcgaaacaat acctgcgaga acgaatactc
     1381 ggtgcgcgtc ctggctcaag ctacgcagca gggatcccag agctcgcagc cctgtcgcac
     1441 ggagtgacag ggccagagtg atggcaccgc ggcggttcac tcacaggtcc tgcacgcggg
     1501 ggccgaacga gcgcagaacc aacgtttccg agcctgcctc cgtatccgtc tcagcgtcgg
     1561 tttgacgctg cacgatgcct actccagggc aaaatacggt ggtggtgcgc ttggacagct
     1621 ggtcattcga cacagcttcg acggtctcca agcagcccgc gaagttaccc aagggcacgc
     1681 tgatcttgcg gtccacactc gtgaccttca cctggccgaa gtcacccgga tacgtcgccc
     1741 cttccgtgag cggcagcttc aggagccagc cgccggtcgc gtggcggagc cccgtatcgt
     1801 cgagttcgag ccggcgcgtt cgcccggcca cgatgagttc gaccgctccc gagcgcgtgc
     1861 ggcggatctc caacgcgagc attcccttat cgccgctcga agagctcgta tcgtaggcgt
     1921 aaacgatccc gtccttgagc gggaagaact gctctacgcc gtgacttgcc gtgggcattg
     1981 cgggcggggg cgcgggagcc accgtcgcgc tctggcacgc catgatcaag cccaagccga
     2041 cgcccgtgat agtgcgttca aacatgtgag ccatacgccg cggcgaccac gcaaatgaag
     2101 agcacgatcg ccgcaaccag cagcagcttc atcagtgggt cgggttcgat gcgacggagt
     2161 agttcctccg cggcggcgcg gatctccgga ttcgtagagc gctcgatcaa ggtcggcgcg
     2221 ccctggcgga gctccctgta gttgccggct tcgaagcagc caatcaaccg ctcgagctcg
     2281 gggtcttccg gaaactcgag caagaaacgg ggccgctcac ggcccttggc atcgaggttt
     2341 gctcgccgcg gggccacctc cggtaaagct ttgttcttcg aacggcgctt tttggtgcgt
     2401 ttctccgccc gctcgggcgt tccagaagag ccgagctccg ccgctcccga tgcgccggga
     2461 tcgccggtcg cagaggaacc ggagttttcg ggcttcggtg caggctcgag cgagccatcg
     2521 gcgggcgccg agggagtacc ggggggagtg tccgaagctt tgctcattag aactggaagt
     2581 acacgaaggg aaccgcttcg gcgctggcca cgttgcggag cagcaccgag acgcaaaata
     2641 gcagagccgc ctgcgccagt gcaggtaaac ggcaaaaccc agcttgcgcg gcggcgaacc
     2701 aacgatcagg gacgaagtgg ctggtgagac cgagcagcat tacgagcaga acgcgcgcat
     2761 ccaagttcgg atggtagagg gtgccttgga agatgcgccc gaagatgatc cgggcctgcg
     2821 tgaaggtctc cgagcggaac aagatccaac tcgcgaggac gaggtgaaac gtgagcacgg
     2881 tctgcaaaac gcggcggggc agagagagcg aacgctgcgc cccgaacgtc gccgctttgc
     2941 gggaaccagg cagatcttgg tatgcacgcg tcagtgccaa cgccgctccg tggagcccgc
     3001 cccaagcgac gaaggtccaa ttggcgccgt gccacagccc gccgagcagc atggtgagca
     3061 gtagattcac gtacgtccgc gtggggccgt ggcgattgcc cccgagcgga atgtacaggt
     3121 aatcacgcag ccacgtagag agcgagatgt gccagcgccg ccagaaatcg gcgatgttca
     3181 cggccgtgta tggcgcgtcg aagtttcgcg gaaagcgaac tccgagcagc aaagcgcagc
     3241 ccgaagcgat atccgtgtac cccgagaagt cacaatagat ctgcagggcg tagccgacgg
     3301 cggccgcgta acactcgagt gacgagtact gcagcggggc ttggaagacc cgatcgacca
     3361 agttgatggc caggaaatcc gcgatcacga tcttcttcac gagaccgatc gcgatcgcaa
     3421 acaagccctc cgcgccggct tcgttgctga agcgacccgg tgccgcgagt tgcggcagca
     3481 gatcccgcgg gcggacgatc gggcccgcca ctaggtgcgg gaaaaacgct acgaatgtga
     3541 ggtattcgag gtaactcgga tgcggctcga tgtcgccccg atacacatcg atgacgtagc
     3601 tcatcgactc gaaggtgaag aaactaatcc cgatcgggag cgcgatctcg agcgtgggtc
     3661 gggagacgcc gaacgtcgtc aacaatggcg aaagcgtgtc cagaccgaag ttcgcgtact
     3721 tgaagaagcc gagcaccgac aggtttacga ccaccgtgag ccacagccaa cgcttgcggt
     3781 gacgcgcatc tggcgcgcgc gcaatcgcgc gtgcgagcca gaagtctagg ctagaggagc
     3841 cgaaaatcag cggcaaaaag cggtagtccc aatgcgcgta gaaggcatag ctcgcgagca
     3901 gcaggaacac gatgcgcgcg acgcgcaagc gcaccaaaag ccaggagact atgaatacga
     3961 cgccgaagaa cttggcgaag gcgagggtat tgaacggcac gtatccggcg agctccgagc
     4021 gtttcggatg ctactatcgc ggcctgcatg gagcgtccgc agcttttttc accggtgccg
     4081 cgtccggcgc ggtttgcgat ccgaagtcag ggccgcatcc ggccactcga gcgaccccga
     4141 ctcctacaag ctggcccgtg ctgactcctg gcgcttctcg gttgagcccc ggcgccaagc
     4201 cgagcccggc gcgggttgct ttcctattgg ccacggttgg ggtcggcgcg ctgctggtgc
     4261 tggcgctata ccgcgctcta gttgggcaga gttcgttcgc agcatggtcg acgctggggc
     4321 tcgcggtgtt gggttgggtc gtcctctcca cgttcggggt gttcttcccg tggctagcga
     4381 tgtacgggcc ggtcgtctcg cgagggccgc gcggcctcaa tcgggtggcg ctcacgttcg
     4441 atgatggtcc gcacccggta accacgcggc gtgtgctcga gctgctgcgc gggacgccgc
     4501 atcgagcgac gttcttcgtg ctggccgata aagtggcgcg ccacccagag gtggtgcgcg
     4561 agatcttcgg cgatggtcac accattgcac tgcacggggc cgtgcacgat cgcctgcatt
     4621 cattccgcgg cgcgaaccgg gtagagcgag agctgcgtgg cgcgcgcgcg gaattggagg
     4681 cgctgatcgg cgagcaagtg agatggtttc gtccgccgct cgggcatacc agtccggcca
     4741 cgatgcgcgg tgtggctcgg gtggggctca acgtgattgg ctggtcgagt cgaggctacg
     4801 acggtttgga gcggacctcc tcgcgagagg tgttgcgacg cgtgctcgga accctgaatg
     4861 atggggcaat cgtgttgctc cacgacgcgg ccgaacgcga tgacttcgag cccaagagcc
     4921 tgctgatttt gcctgagctc ctggccgcgt tggatgcccg agggttacga agtgtagggc
     4981 tgaacacttg gctcaaatga gcttcgaagc gcgacgtagg ctgttgcggg cttttcccgc
     5041 tgctatagtc cagcgcccgt aaagataccg gcactggaaa ctcccaccca ctctgactcg
     5101 tagcaaaacc tagaatgacg gactcaaccg gctcggcccc gccgcgaggc atgaaaactg
     5161 gtggcaatga ccgcgatacc agcgaaaggc ccgccagtgg ccccgcgccg cgagccacgc
     5221 agccgccgac gacgatggct ccgcgccgac gaaggcgctg gtgggtttgg ggtttggctg
     5281 ccacttcggg gttggcggta gcggtttggg cgtggcccaa aggggcttcc tcggccttgg
     5341 ccgcagaagc gccagacgtg ccgcgagcgg agggcaagcg cgtcatgttt tcggacaatt
     5401 acgccaagcg tattgctctc gaaaccgtca gcgtgggcac gcatgacgtg gtgccggccg
     5461 tttcggtggt gggtactgtc gatttcgatc ccgagtacgt cgcccgcgtc ggtacacggc
     5521 tgcgcggagt agtgcgctcg gtttccaagt tcgaaggtgc gcgcgtgaag cgtggggatg
     5581 tactggcttc gatcgacagc ccggagctcg gtgacgcgca agccagcgtg gcttctttac
     5641 gtgcggagtt caacgcactc gcgagatcgc gagatcgtga gcaagcactc tcggcgcgca
     5701 atttgacgac gccgcgtgaa gccgaagagg cgacggcgca ggcgagccgt tcacaggcct
     5761 tgttacaggc ggcggagcag cgggtttcgg cgctggcggg gcgggcagcg agccccaagg
     5821 cacagctcgg cgtgcatgac gtcacctcgc cgttggacgg cgtggtcgtc gagcgtcatg
     5881 tggccaaggg ccagctcgtg tcttccgatc acttcgcgtt cttgatcgcg aatctcgacc
     5941 acctttgggt ggagctctcg gtgttcgagc gccacttgcc cggcatcaaa gtgggagatg
     6001 aggtggagct ccgatcgaac tccagcgcgc acgccgagcc ggtcaaggga cgcgtcgctc
     6061 gggtcggcgc cgtgctcgag gcggagagcc gcagcgctca ggtgcgcgtc gagctcaaga
     6121 acgaacagcg tcggttcgtt ccaggtcaat ccgtggttgc ggtgatccgc gcgaccgcgg
     6181 ctgaggccga tgacgttccg accgtccctc acagcgcagt cgtgttcgta gacggagtgc
     6241 ccaccgtgtt cgtggcagat agccccacgt ccgtgctcgt gaccgaggta gaactcggtg
     6301 aaagcaatgg ccaggtgctg cacgtgaagc gtggattgtc cgccgaccaa cgcgtggtga
     6361 ctcggggagc aactgcgctg agaaatgagc tgttccgcta gcgatcgggc agtcgccgaa
     6421 gcgccaagcc ttgcttcgaa gcatcttgca ggtcgcgcgg gagggtgaaa ttccctttca
     6481 ttgagttgcg atagcagcgc gcaactggag taagctcccg ctggggatga aggttcagcg
     6541 cctgcatccc tccgtctcga agcctattcg tctcagggag tttcaatgct tgcagctgtg
     6601 gtggcctggg ccattcgcgc acgacgcgct gtcctggtcg gcttggtcgt gttgattgcg
     6661 tgcgggattc aggccgctcg atcgttgccc atcgacgcgc tgccggatgt atcgacgatc
     6721 caggtcagtg tgctcacgac tgcgtcgggg ctgtcgccgc tcgaggtcga gcgcaccgtc
     6781 actatgccgg tcgaagtggc tttgaacggt accccgggag gcgtagagct gcgcagcgtt
     6841 tcccgttcgg gtctgtccgc ggtgacggtg gtgttcaagg acgggaccga cgtctggttt
     6901 gcgcgccagc tcgtgctgga gcgcttgcgc ggcatagagc tgccgcctgc ggccgcgatg
     6961 cccgagctgg cccccgtgtc caccggacta ggagaaatct accagttcgt cgtgcgcagt
     7021 gatcaacact cgccgatgca gctgcgcacc ctgctcgatt gggagatcgt tcccaagctg
     7081 cgcagcgtgc ccggcgtggt ggaggtgaac accgccggcg gcgagctcaa gcagttccag
     7141 gtggacgtcg atcgcgttcg gctcgaagcg cgcgggctat cgctcgccaa cgtggtggac
     7201 gccttgcgca acgccaacgt cagcgtcggc ggcggttacg tggagcgcaa caaagagtcc
     7261 ttcaccgtgc gcggcgaagg catgctgcgc gacatccagg atatcggtaa ggtgatcgtc
     7321 aagacgagcc cgaacgcggc gcccgtcttg atccgtcacg tcgccgacgt gcacatcggc
     7381 tctgctttgc gctacggcgt aatcactcaa gacggcaagg gtgaagcggt gaccggcatc
     7441 gtgatgatgc tgctcggcgc caatagcaaa caggtcgtga ctgacgtggc cgagcgggtg
     7501 aagcaaatcc agaaggagtt gcccgcaggt gtgcgcgtcg aggtgatcta cgaccgcgca
     7561 gatttcgtgg gccgcacgct cagcacggtg atgaagaacc tgctcgaggg cgtcgcggtg
     7621 gtctcggtcg tattggtgtt gttcct
//
LOCUS       DASTKU010000646         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_570133, whole genome shotgun sequence.
ACCESSION   DASTKU010000646 DASTKU010000000
VERSION     DASTKU010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570133"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VFQ61_22580"
     CDS             complement(<1..422)
                     /locus_tag="VFQ61_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957309.1"
                     /translation="MNAFIPLSPVDGCSGGGKINLCSTESLVAIEEMSSRDPKIVEVV
                     PAVDMPAKLSNDEFFARGASPGKTTIDFRGRFDDGSVRSATLDIEVASVTRLDIESRC
                     TTEKNGVVLAAPGELASFEVLMYNQQRQLAGWHPSAIDS"
     gene            919..>2286
                     /locus_tag="VFQ61_22585"
     CDS             919..>2286
                     /locus_tag="VFQ61_22585"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HET9957310.1"
                     /translation="MRRFEFSEGNSNKFWQVEQKGESLLIQWGKIGTNGQSQTKDFTN
                     DAKAKSEADKLIAEKKKKGYVEVKDVAPAGTASTRAPAIASSAPSAAAMPTASNANAS
                     AAASKKEPAPRDRTAQSKDQADGEGIPSGTEGRAADESAPTPAPSKESSLDQAFKPHP
                     VVVLQDEHRAERDTWRDLTAEEIPSASDLLSSLHAFIVKQRKDDKAWSEGKNHADAHG
                     KAVADYALESYAKGKVPTKPDAALDGAFTALVRTTFTMRLFALWHGLEYALDAFVESF
                     SNEWHHEYKSNAVSPAWVARCDPATNWAPFAGSGEKRIQSLAGVVDAAEASARNGAHA
                     HAEKLRRSAALPARVGLSLVFADTKWAAQDALEVLALKDQDIYAGRELLYLLRERRLL
                     DAFPTPEYGRHPISLLVNAGLECVGVLIAWLDNQYSRDEAARALSCVERVESARALVT
                     VLDKKD"
BASE COUNT          466 a          707 c          740 g          373 t
ORIGIN      
        1 gaatcgatcg cgctcggatg ccaaccagct agctgccgct gttgattgta catgaggacc
       61 tcgaacgaag cgagttcgcc cggtgcagcg agcacgacgc cattcttctc ggtcgtgcac
      121 ctcgactcga tgtcgaggcg agtaacgctt gccacttcga tgtccagtgt cgcgctgcgc
      181 accgagccgt cgtcgaagcg cccacggaag tcgatggtcg tcttgccggg actcgccccg
      241 cgcgcaaaga attcgtcgtt cgaaagcttt gccggcatat ccaccgccgg cacaacctcc
      301 acgatcttcg gatcccggga ggacatctct tcgatcgcaa ccaaggactc ggtgctgcaa
      361 aggttgatct tgccaccccc cgaacagcca tctactggcg agagcgggat gaaggcattc
      421 acggccagtg gttgcgtgcc gaggctcgta ttgaacgccg cctcgtcgtc gtgctgaacg
      481 cacgaaaccg tcggccatac tgcgaaaacg acaacccacc cacggcgcgg attccacatc
      541 tcctctgaac tgcagcaagg cctatgccag ccgccctgcg ctagggaatt ggggatctta
      601 tcgagtcgag cgcgtaccgg cagtggcggc gagtgcgatt tggccgtcgc acctgcctcg
      661 cgcgcggccg gctctgccgg gttcaccccg caattcgaaa gtccagtgtt ggaggcaaag
      721 gccgatctgc tcacttggac gtaggcgcgc gagccgtgac attccaacgg gtgagcgaag
      781 cgatcggccg gagggggcgt ttccccgcgg cttcatggcc cccgcacacc cgagctgagc
      841 tcggcgcggc gggtatgggg aaccgctccc ggaggattgg gaatgacccg cgctcgtgcg
      901 ccgtataggg tcctttgcat gcggcgtttt gagttttcgg agggtaactc gaacaagttc
      961 tggcaggtgg agcagaaggg tgagtcgctc ctcatccaat gggggaaaat cggaacgaac
     1021 ggtcagtcgc aaaccaagga ctttacgaac gacgcgaaag cgaagagcga agccgacaag
     1081 ctcatcgccg agaaaaagaa gaagggttac gtcgaggtga aggacgtagc accggcgggg
     1141 acggccagta cccgcgcgcc ggccatcgcg agctcggcgc cgagcgccgc ggccatgccc
     1201 acggcgagca acgctaatgc ttcggcagcg gcttcgaaga aggagccggc gccacgagac
     1261 aggacggccc agagtaagga ccaagccgac ggcgaaggca tcccgagcgg cacagagggg
     1321 cgagccgcgg acgagagcgc gccgacgccg gctccatcga aagagagctc tctcgaccaa
     1381 gcgttcaagc cgcaccccgt ggtcgtactg caagacgagc accgcgcaga gcgcgacact
     1441 tggcgcgacc ttacagcaga ggagatcccc agtgcatccg acctcctgag ctcactccac
     1501 gccttcatcg tcaagcagcg caaagacgac aaggcctggt cggaggggaa aaaccatgcg
     1561 gacgcacatg gcaaggccgt agctgactat gcgctcgaga gctacgcaaa gggcaaggtg
     1621 cccacgaagc cggatgctgc gctggacgga gcgttcacgg cgctcgtccg aactaccttt
     1681 accatgcgcc tcttcgcctt gtggcacggc ctcgagtacg cactcgacgc cttcgtcgag
     1741 agcttctcca acgagtggca ccatgagtac aaatccaatg ccgtcagccc agcgtgggtt
     1801 gcgcgttgcg atcctgccac gaattgggct ccgttcgccg gatcgggaga gaagcggatt
     1861 cagtccctag cgggagtggt cgatgcagcc gaggcaagcg ctcgcaacgg ggctcatgcc
     1921 cacgcggaaa agctccgcag gagcgcggct ctgccggcgc gtgtaggcct ttcgttggtc
     1981 ttcgcggata cgaagtgggc ggcccaagac gctttggaag tgctcgcgct gaaggaccag
     2041 gacatctacg cgggacgcga actcttgtat ttgttgcgag agcgccgcct gctcgacgcg
     2101 tttcccacgc ccgagtatgg acgccacccc atttcgctgc tggtaaacgc ggggctcgaa
     2161 tgcgtaggag tgctgatcgc gtggctggac aaccagtact cgcgcgacga agcggcgcga
     2221 gcgctctctt gcgtggagag ggtcgagtcg gcgcgcgctc tcgtgacggt gctggacaag
     2281 aaggac
//
LOCUS       DASTKU010000647         9541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_124642, whole genome shotgun sequence.
ACCESSION   DASTKU010000647 DASTKU010000000
VERSION     DASTKU010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9541
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124642"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VFQ61_22590"
     CDS             complement(<1..901)
                     /locus_tag="VFQ61_22590"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET9957311.1"
                     /translation="MGSVEVQFAALTDVGQQREQNEDNFLVDKKLGLYVVCDGMGGHA
                     AGEIASALCVRVIHDELRKEGDLLRDYVTGRTGGSRVSKRDITNLLEFATNRASSRIH
                     AEALKDPKKRGMGTTLLIVLLLGSQAFILWVGDSRVYHLRGTALTQLTEDHNVYNELI
                     RRKKMAREQVEKLTQKNAITRAVGVYEHVEPETLVVDVVRGDRFLLCSDGLSSYFEDD
                     LVGLASTLSTPDADEAVRSLVETANQRGGKDNITAIVVTVGSVLARDEGRANRLKLQQ
                     TLLARMPLFRPLSERELLRVLQVT"
     gene            complement(982..1635)
                     /gene="radC"
                     /locus_tag="VFQ61_22595"
     CDS             complement(982..1635)
                     /gene="radC"
                     /locus_tag="VFQ61_22595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET9957312.1"
                     /translation="MPDHRERALRVGVEVLSDQDLLAIVLGTGSTGEPVNLLAATLLD
                     SEGGVTGLSRNTPHRLAGRRGIGPAKAVRLAAAFELGRRAQIHEFGPDPLTVRHFEQV
                     VAWARPRLATLDHEEVWLLMLDAKNGLRSVRRISQGGSHACALTARDILRPALRDGAS
                     AIVLIHNHPSGDPTPSPEDVEMTKNVMAACDVVGLALFDHVVVARRGACSMFEAGTL"
     gene            complement(1848..2588)
                     /locus_tag="VFQ61_22600"
     CDS             complement(1848..2588)
                     /locus_tag="VFQ61_22600"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET9957313.1"
                     /translation="MTLTAATAAALIIGNELLSGKIAEANLFELARLLRVQGIRLQRA
                     VLVPDEIEVIEGEIRSLRQSADVVFTSGGVGPTHDDVTVAAAARAFGVDVVPDPLLTE
                     MLREYYGERCNEAHLRMALVPRGATLLRSADVRWPAILMGNVFLLPGVPEIFRSKLPL
                     IKSYVQGPEPFFSRAVYLSLEEPEITAALDSTVPHHPDVEIGSYPQWVGSSYKTKVTF
                     DGRRAAPVQLASDEFLTKVERSAVVRVE"
     gene            complement(2585..2842)
                     /gene="xseB"
                     /locus_tag="VFQ61_22605"
     CDS             complement(2585..2842)
                     /gene="xseB"
                     /locus_tag="VFQ61_22605"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET9957314.1"
                     /translation="MREKTSQEIEAPSFEAALGRLSEIVERLESSELPLEESLRLFEE
                     GISLSRIAQTRLNAAEKRVEELLSVNDDGSPVVRELGAEDR"
     gene            complement(2903..5095)
                     /locus_tag="VFQ61_22610"
     CDS             complement(2903..5095)
                     /locus_tag="VFQ61_22610"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9957315.1"
                     /translation="MKRTASGLDPDAASVTAPGLDLDAVKVATSGLRGDTSRVLARIA
                     LLLCFQAGLAMPANAATNPKSTDAPSLLDRVGALHLEKSLRARDARVRERSIERLGAL
                     GTSRALELLVRALDANGSAESPRERLLAVRALAAHVREPSVRDCLVRVMTGVSANAER
                     ADPIQALLRDTAALALAASGDARAEDALVKALRQPGRVAQAAARALVAHPPARLDWAL
                     EKRFTPSLELVSALEELGDPRGIEVFRDAIRRGSPELRARASVALARLGQNEAYTLAE
                     RFAAPNSPAVLQLAAAEIMIGAPPRSGLPQWTAKLLASLLARPETRERALELATRGVP
                     GLGSALEAQASRVSGEELQTLVIALTRTQDASTWPALGRLLEQPPNAELMSAEVTAYA
                     LSYSESDAAERLLLRASEGKAAPVAARAAVLQATRFGLGASELKAQLVSWLASSRPPE
                     RAIAAWGLGLLEPERLPSLLRSAHDEVVQAAARAAPFVSAAELLVEPLASAASAATRN
                     QLAIALADERARRRVPNDVLEKLVSEGNVAAPLAVFALCTRDSPSMRDRLRSFLTAPD
                     PLLRAHAALGFGWSREPSALGVLTTSYAFEADARVRRALVHAMALRREEVRAHWLKQA
                     AALDPDTRVRELAELALAGVRPATFAPGHGTFWVHLDTFADPKQRTGARNSGLERGAA
                     SITIAGGLALPVLADPDGIIALAGLPEGPVSLTLALAPHAGKPAEEKR"
     gene            complement(5092..7035)
                     /locus_tag="VFQ61_22615"
     CDS             complement(5092..7035)
                     /locus_tag="VFQ61_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HET9957316.1"
                     /translation="MLRGFRAALLASVWLACSGLSQSAWAAPPPSLDLRGFNASTDPR
                     AALYLEPAATPGHLQWNAGVWASYANRLVELEADGHTIAVPVRHQWSVDYVAGLGLTD
                     RLAIGVVVPTVAYQTGDDVRGLLPDARALPKASLGDLSLTVKATLVPTRTLGGFALAA
                     RGRLSLPTGSAASYVSETAASGELGLLGELGLVALTVRATAGMRVRGAERAYADATLG
                     QSLPWGAQLEFLPQALGWDDEGRWRWNLEAHGAVALTSGFAAAAVSPALMGASARYAF
                     GDVGVIGGAELPLNDALGAPQVRAVLGFSYAPRVIDTDEDGVPDKRDECPELAEDIDQ
                     FQDADGCPDFDNDDDGVPDDQDKCPKEKEDSDEYQDDDGCLDPNNDADALPDAQDKCP
                     NEPGPAETSGCPAKDLDLDGIDDNRDRCPKAAEDRDAFEDEDGCPDPDDDADGVPDVE
                     DACVREPGPARSEPALHGCPSPDRDGDTLEGELDKCPEQAETFNGEADEDGCPELESK
                     AKPLASFVRQRVGRDQTTIRLELAQPLVFDAQNALDSKSERLARSIAAELNRYPQSVV
                     LVGVRPARPTSEAEQTALNRSFAVVELLRHYTHRDEVAETIHWKAVEKLPRPARADIG
                     FLVLSKDTNIPEAAPVAPARRTP"
     gene            7172..7828
                     /gene="hisG"
                     /locus_tag="VFQ61_22620"
     CDS             7172..7828
                     /gene="hisG"
                     /locus_tag="VFQ61_22620"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET9957317.1"
                     /translation="MRRPLTVAVPKGRVVKALLPFFKRAGIDTSPLEADDRRLVREAA
                     DGSVRFLLLKPDDVPTYVEYGTAELGISGRDVLHERAYDLYQPLDLGVGRCRMVVAGP
                     RDAQIPSVPRIATKFPRIASEHFASRGTQAEIILVQGSVELAPLVGLSDLIVDLVESG
                     ATLRDNDLVEYETITDISSVVIANRSLFKLRHAEIQPLLDRLRKAVSDQAAERASAAP
                     "
     gene            complement(7894..>9541)
                     /locus_tag="VFQ61_22625"
     CDS             complement(7894..>9541)
                     /locus_tag="VFQ61_22625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957318.1"
                     /translation="ASWSWAAFFLYPGILVYDSNLSTGADHLAAIWAPAGLLLVLRLW
                     TTPDLRAKALSFKAALLGALAGGAALTKYSAICAAAPLLLGLIWACIRPRWRELSTAA
                     SATKSASTFSWVPIGVGLAAFVLVTSQHWLKNWVWHGDPLYPILRKYLPAHPWHADAE
                     AYFRAFKKGALLKPNRGALGVWQALRANVTLGFEVNDYDFHGQYPTFGYLFPITALLS
                     FLVPSRWRVRGLAVLCMCGVTIWFATNHRDRYLQAILPWMVAVTFCALRVAWRQHGAS
                     VRAGLVLLVGSQLTTSLDVWLFPSHMAEGAHPILPLYDFVKLGMEGKYAERAQVPSAW
                     GFADWVALGSKLPRGSKVLVHEDRLWTGLDAPVVTDEAAWQAGLHYGSLRDTRAVHQA
                     LREYGVTHIVTGHPHGDGGFHALAGELLFWDYVRNQTCPVARSGHLELHRLPSSAPER
                     GAPERVLVMTCQASEPAGVYDLRALRSATAPPAAPFRPIDVEAVPEPIWEGVEYVVTE
                     DECGNWETGTRAFTRLYTREKLTFWRRAATAPRPVGCKSK"
BASE COUNT         1662 a         3220 c         3031 g         1628 t
ORIGIN      
        1 ccgtgacctg cagaactcgt agcaattcgc gctcggatag tgggcgaaac agaggcattc
       61 gggccagcaa cgtctgctgg agcttgaggc gattggcgcg gccttcgtcg cgcgcgagca
      121 cgcttcccac cgtgaccacg atcgccgtga tgttgtcctt gcctccgcgc tgatttgcgg
      181 tctccaccag actacgcacc gcttcgtcgg cgtcgggggt gctcagggtg ctggcgagcc
      241 caactaggtc gtcctcgaag taggagctca ggccgtcgct gcagagcagg aagcgatccc
      301 cgcgcaccac gtctacgacc agcgtctccg gctcgacgtg ctcatagacg ccaaccgcgc
      361 gggtgatggc gttcttctgc gtgagcttct cgacttgctc gcgcgccatc ttctttcgcc
      421 ggatcagctc gttgtacacg ttgtgatctt cggtgagctg agtcagggcc gtgccgcgga
      481 ggtgatacac gcggctatcc cccacccaca gaatgaacgc ttggctcccg agcagcagca
      541 cgattaggag cgtcgtgccc atgccccgct tcttgggatc cttcagtgct tcggcgtgaa
      601 tgcggctaga cgcgcgattg gtcgcgaact ccagcaagtt ggtgatgtca cgcttgctga
      661 cgcgtgagcc accggttcgc ccggtcacgt agtcgcgcag caaatcgccc tctttccgga
      721 gctcgtcgtg gatcactcgc acacaaagcg cgcttgcgat ttccccagcg gcgtgccccc
      781 ccatgccgtc gcacacgacg tagagcccga gcttcttgtc gaccaagaag ttgtcttcgt
      841 tttgctcgcg ttgctggcct acgtccgtaa gagccgcaaa ctggacttcg acgcttccca
      901 ttcggatgaa gaggatactc aagggccatt ggcggggcaa aggggccggc ccgcccgagc
      961 acccgaattt cgacagcacc gctacagtgt tccggcttcg aacatggagc atgctccgcg
     1021 gcgagccacc acgacgtggt cgaacagcgc caggccaacg acatcgcacg ccgccatcac
     1081 gttcttggtc atttccacgt cctctgggct cggcgtggga tcaccactcg gatggttgtg
     1141 aatcagcacg atggcgctcg cgccgtcgcg caatgccggc cgcaagatgt cgcgcgcggt
     1201 cagcgcgcag gcgtgcgagc ccccctgcga aattcggcgc accgagcgca acccgttctt
     1261 cgcgtcgagc atcaacaacc aaacctcttc gtggtccaac gtggcgagtc gcgggcgagc
     1321 ccacgccacc acttgctcga agtgccgcac ggtcaaaggg tcgggaccaa actcgtggat
     1381 ttgagcgcgc cgcccgagct cgaacgccgc cgccaagcgc acggccttgg ccggcccgat
     1441 gccgcgtcga cctgccaacc tatgcggtgt attgcgcgac agaccggtga ccccgccttc
     1501 gctgtcgagc agcgtcgcgg ccaacaagtt gactggctct ccggtactgc ccgtgccgag
     1561 cacgatagcc agcaaatcct gatcggacag aacctcgact ccgacgcgca gcgctcgttc
     1621 ccgatgatcc ggcatgaata acctcgccgt cccggagttc acgtcgcgtg ccggtcctcg
     1681 gaactggttc tttccgcgaa ttcgagcgga aattcggcgg ctactgcgtt ggccgccgct
     1741 cgacggttgg cgcaagttcg ccagttctgc agcaactggt tgcgcggcgg ccagttctgc
     1801 gcaccctgtt ttgcggatcc tgttttgcgc aaaccagtgg cgaggctcta ctctacgcgc
     1861 acgacggcgg agcgctccac cttggtgagg aactcgtcag acgcgagctg aacgggcgcg
     1921 gcgcgacgcc cgtcgaaggt caccttggtc ttgtaagagc tgcccaccca ctgtggatag
     1981 gagccgatct ccacgtccgg atgatggggg accgtcgagt ccagcgcggc cgtgatctcc
     2041 ggttcctcca aactgagata cacggctcgg gaaaagaacg gctccggccc ctgcacgtaa
     2101 ctcttgatta ggggcagctt gcttcggaaa atctccggca cgcccggcag caagaacacg
     2161 ttacccatga gtatggccgg ccagcgaacg tcggcagagc gcagtaacgt ggccccccgt
     2221 ggcacgagcg ccatccttag gtgcgcttca ttgcagcgct cgccgtagta ctctcgcagc
     2281 atctcggtca acagcgggtc ggggaccacg tccactccga aggcgcgcgc tgcggcggct
     2341 acggtgacgt cgtcatgcgt tgggcccacg ccgccgctcg tgaacaccac gtctgcgctt
     2401 tgccgcaagg accggatctc gccctcgatc acctcgatct cgtcgggcac gagcacggcc
     2461 cgttgcagcc ggatgccttg tacgcgcagc agcctcgcca gctcgaaaag gttggcttct
     2521 gcaatcttgc ccgacaagag ctcgttgccg atgatcagag cggcggccgt cgcggcggtg
     2581 agcgtcatcg atcttcggct ccgagctcgc gtacgacggg ggagccgtcg tcgttgaccg
     2641 agagcagctc ctcgacccgc ttttccgcgg cattcaggcg cgtctgagcg atcctcgaaa
     2701 gactgatgcc ctcctcgaac aaacgcagcg actcttcgag aggtagctcg gagctctcca
     2761 agcgttcgac gatttcgctc aagcgcccga gcgcggcctc gaagctcggg gcctcgattt
     2821 cttggctagt tttttcgcgc atgttagcgg tgggcgcggc ggtcggtcgc acgacggaag
     2881 atttggcggc agggggcatg gcttagcgct tctcctcggc aggcttgcct gcatgcggcg
     2941 cgagcgcaag cgtgagtgag accgggcctt cgggcaagcc tgctaatgca atgatcccgt
     3001 ccggatcggc cagcaccggc aaggccaagc caccagcaat cgtgatgctc gcagcgcctc
     3061 gttcgagccc ggagttcctg gcgccggttc gttgcttggg atccgcgaac gtgtcgaggt
     3121 gaacccaaaa cgtgccatgc cctggcgcga aggttgcggg ccgcaccccc gcgagggcaa
     3181 gctcggcgag ctcgcgcacg cgcgtgtccg gatccagcgc cgcggcctgc ttcagccagt
     3241 gcgcgcgcac ttcctcacgc cgcaaggcca tcgcgtgcac gagcgcgcgc cgtactcggg
     3301 catcggcctc gaaagcgtag ctggtcgtga gcacgcccag cgccgaaggt tcgcgactcc
     3361 agccgaagcc gagcgcggcg tgggcgcgca gcaacgggtc gggcgcggtc aagaagctgc
     3421 gcaaccgatc gcgcatcgac gggctgtcgc gcgtgcagag cgcaaacacg gccaacggcg
     3481 ccgccacatt gccctcggac acgagctttt ccagcacgtc attgggaacg cggcgtcgcg
     3541 cccgctcgtc cgccagcgcg atggcgagtt gattgcgagt ggctgcggag gcggcgctcg
     3601 ccaaaggttc gacgagcaac tcggccgcgc tcacgaaggg agccgcgcga gcggcggctt
     3661 gcaccacttc atcgtgcgca ctacgtagca ggctcggcaa acgttccggt tcgagcaggc
     3721 cgagtcccca cgccgcaatg gcccgctccg gtggtcggga cgaggcaagc caacttacga
     3781 gctgggcttt cagctctgaa gctccgagcc cgaaccgcgt ggcttgcaag acggcggcgc
     3841 gcgcggcgac tggcgcagct ttgccctcgc tggcccgaag gagaaggcgc tccgccgcgt
     3901 ccgactccga gtaagagagc gcgtacgctg tgacctctgc gctcatcagc tccgcgttcg
     3961 gtggctgttc gagcagtctg cccagggcag gccatgtgct tgcatcttgg gtgcgcgtga
     4021 gcgcgatcac caaggtttgc agctcctccc cgctgactcg cgacgcttgt gcctcgagtg
     4081 cggagcccaa gccaggcact ccgcgcgtgg ccagctcgag cgcccgctcc cgcgtctcgg
     4141 gtcgggccag caaggaagcg agtaactttg ccgtccactg cggcagccca gacctggggg
     4201 gcgcgccgat catgatctca gcggccgcga gctgtaagac agccggagaa ttgggggccg
     4261 cgaagcgttc ggctagagta tatgcctcgt tttgaccgag acgcgccagt gccacgctgg
     4321 cgcgggcgcg gagttcgggc gagccgcggc gaatggcatc acgaaacact tcgatgccgc
     4381 gcggatcgcc gagttcctcg agcgccgaca ccaactcgag gctcggcgtg aagcgctttt
     4441 cgagcgccca atccaagcgg gcaggcggat gcgccaccag cgcgcgcgcg gccgcttgcg
     4501 ccacacgacc gggctgacgc agcgctttga ccaatgcatc ttcggcacga gcatcgccgc
     4561 tcgccgccag cgcgagcgcg gccgtgtccc gcagcaatgc ttgaatcgga tcggcgcgtt
     4621 ccgcattggc gctcacgccc gtcatgaccc gcaccaggca atcgcgcacg ctcggttcgc
     4681 gcacgtgtgc ggccagagcg cgtacggcga gcaaccgctc gcgcggggat tcagcgctcc
     4741 cgttcgcgtc tagcgcccgc accaagagct ccagtgcgcg cgaagtgccc agggccccga
     4801 gccgctcgat ggaacgctct cgcacccgcg catcacgcgc gcgcagggat ttctccaggt
     4861 gcagagcacc cacgcgatcc agcagggatg gcgcgtccgt gctcttcgga tttgttgcgg
     4921 cgttcgccgg catcgccaat cccgcctgaa agcagagtaa gagggcgatc cgagcgagga
     4981 ctctgctcgt gtccccgcgg agccccgacg tcgcgacttt caccgcgtcg aggtcgagcc
     5041 ccggcgccgt gacgcttgca gcgtcggggt cgagccccga cgctgtgcgt ttcaaggcgt
     5101 cctccgcgcg ggcgcgactg gcgccgcctc cgggatgttc gtgtccttgg agagcaccag
     5161 gaatccgatg tccgcgcggg cgggcctcgg cagtttctcg accgccttcc agtgaatcgt
     5221 ctcggccacc tcgtcgcgat gggtatagtg gcgcagcagc tcgaccaccg caaaggagcg
     5281 gttgagcgcg gtctgttcgg cttccgacgt cggccgagcg gggcgaaccc ccaccaacac
     5341 cacgctctgc ggatatcggt tcaactcagc cgcgatcgag cgagccagtc gctccgactt
     5401 cgagtccaag gcgttctgcg catcgaatac gagcggctgg gccagctcga gccgaatcgt
     5461 cgtttgatcg cgcccgacgc gctgccgcac gaagctcgcc aacggcttgg ccttgctctc
     5521 gagctcgggg cacccgtctt cgtccgcttc tccgttgaag gtttcggcct gctccgggca
     5581 cttgtccagc tccccttcca aggtatcacc gtcgcgatcc gggctcgggc agccatgcag
     5641 ggcgggctcc gagcgggcgg gcccgggctc acgcacacag gcatcctcta cgtccggcac
     5701 tccatcggca tcgtcgtcgg ggtccgggca gccgtcttcg tcttcgaagg cgtcgcgatc
     5761 ctcggccgct ttcggacagc gatcgcgatt atcgtcgatg ccgtcgaggt cgagatcttt
     5821 tgcggggcaa cccgacgtct ccgcaggacc gggctcgttc gggcatttat cctgggcgtc
     5881 cgggagtgcg tcggcatcgt tgttggggtc gaggcagccg tcgtcgtcct ggtactcgtc
     5941 ggagtcttct ttctccttcg ggcacttgtc ctgatcgtcc ggaactccgt cgtcgtcgtt
     6001 gtcgaagtcg gggcagccgt cggcgtcttg gaattgatcg atatcttcgg ccagttcggg
     6061 gcactcgtcg cgcttgtcgg gcactccatc ttcatccgtg tcgatcaccc ggggcgcgta
     6121 cgagaatccg agcaccgctc gcacctgcgg cgccccgagc gcgtcgttca acggtagctc
     6181 ggcaccgcca attacgccga catccccgaa cgcgtagcgc gcagacgcgc ccatcaacgc
     6241 gggcgaaacc gccgccgccg caaaaccaga cgtcaaggcc accgcgccat gtgcttccaa
     6301 gttccaccgc caacggccct catcgtccca gcccagcgct tggggtagga actccagctg
     6361 cgctccccag ggtaggctct gtcccaaggt cgcgtccgcg tacgcgcgct cggcccccct
     6421 tacgcgcatg cccgcggtcg cgcgcaccgt cagcgcgacg agaccgagct cacccagtag
     6481 accgagctct ccgctcgcgg cggtttcaga aacataggac gcggcgcttc ccgtcggcag
     6541 agataggcga ccgcgcgctg cgagcgcgaa ccctcccaat gtgcgagtgg gcacgagtgt
     6601 cgctttcacg gtcagcgaga gatcgccgag cgacgccttg ggaagcgcgc gggcatccgg
     6661 cagcaggcct cgaacgtcat cacccgtctg gtatgcgacc gtcggaacca ccacgccgat
     6721 cgcgagccga tcggtcaagc ctagtcctgc cacgtagtcg accgaccact gatggcgcac
     6781 cggaacggcg atcgtatgtc cgtccgcctc gagctccact agacggtttg cgtacgaagc
     6841 ccagacgccg gcgttccatt gcaggtgccc tggagttgca gcaggttcca agtagagcgc
     6901 ggcgcgcgga tcggtcgagg cattgaatcc gcgcaggtcc aaggaaggcg gcggcgctgc
     6961 ccaggcgctc tgcgaaagcc ccgagcacgc cagccaaacc gaagccaaaa gtgccgcccg
     7021 aaagcctcga agcatgagtg acccaagcac tatcatgccc cctgaaggct gcgaaacccg
     7081 gatcccgcca gtttggggct gggcccatcg gccaacgctt ccgaattagc ttgctttcgc
     7141 gcggctcggc taccaaagaa tgcgggcctc catgagacgc ccgctgaccg tcgccgtgcc
     7201 caaaggccga gtcgtcaaag ccctgctgcc gttcttcaaa cgtgcaggca tcgacacgtc
     7261 tcccctggaa gcggatgacc gtcgcctcgt gcgcgaagcg gcagatggct cggtccggtt
     7321 tcttttgctc aagccggatg atgtcccgac ttacgtcgag tacggcacgg ccgagctagg
     7381 tatcagcggc cgcgatgtgc tgcacgagcg cgcgtacgat ttgtatcaac cgctcgatct
     7441 cggggtgggt cgctgccgca tggtagtagc cgggccccgg gacgcacaaa tcccgagcgt
     7501 gccgcggatc gccaccaagt tcccgcgaat cgcttccgag cactttgctt cgcgcggcac
     7561 tcaggccgag attattttgg tgcagggctc ggtggagctc gcgccactcg tgggtctgtc
     7621 cgacttaatc gtggatctag tggagtcggg agcaacgctg cgcgacaacg acttggtcga
     7681 gtacgagacg atcaccgata tctcgagcgt cgtgatcgcg aaccgatcgc tattcaagct
     7741 gcggcacgcg gagattcagc ccttgctaga tcgcttgcgc aaagccgttt ccgatcaagc
     7801 cgccgaacga gcctccgcgg caccgtagcc tgaacttcgt cgacggagag cacctgcgtc
     7861 gagtcggcgt gaatccaggc ttgctcgtgc tcgctacttc gatttgcacc cgacggggcg
     7921 gggcgccgtc gcggcgcgcc gccagaaggt gagcttctcg cgcgtgtaga ggcgagtgaa
     7981 cgcccgggtt ccagtctccc agttgccgca ctcgtcttcc gtcaccacgt actcgacccc
     8041 ttcccaaatc ggctctggca cagcttcgac gtcaatcggc cgaaatgggg ccgccggcgg
     8101 agcagtcgcg gaccgcaacg cgcgcagatc gtagaccccg gcgggttcgc tggcctggca
     8161 agtcatgacg agcacccgct cgggcgcgcc ccgctcgggc gccgagctcg gtaggcgatg
     8221 caattcgagg tgacctgacc gcgccacggg gcacgtctgg tttcgcacgt aatcccaaaa
     8281 cagcagctca cccgcgagcg cgtggaaacc gccgtctccg tgcggatgtc ccgtcacgat
     8341 gtgggttacg ccatactcgc gcagcgcctg atgcacggcg cgcgtgtcgc gcaatgagcc
     8401 atagtgcaga cccgcttgcc aggccgcttc atcggttacc accggcgcat cgagacccgt
     8461 ccaaaggcga tcttcgtgga ccagcacctt ggacccgcgg ggcagcttcg agcccaacgc
     8521 gacccaatcg gcgaagcccc acgccgacgg tacctgcgcg cgctccgcgt acttgccctc
     8581 cattccgagc ttcacgaagt cgtagagcgg caggatggga tgagcgcctt cggccatgtg
     8641 cgaaggaaag agccagacat ccaacgaggt ggtgagctgg gagcccacga gcagtaccaa
     8701 ccccgcgcgt accgaagccc catgctgccg ccatgccacc cgcaacgcgc agaacgtgac
     8761 cgccaccatc cagggcaata tggcttggag gtaacgatcg cggtggttgg tcgcaaacca
     8821 gatggtcacc ccgcacatgc acaatacggc gagcccgcga acccgccagc gcgagggcac
     8881 taagaagctc agtagcgccg tgatcgggaa gagatatccg aaggtcgggt attgaccatg
     8941 aaagtcatag tcgttgacct cgaaacccaa cgtgacgttg gcgcgcagcg cttgccacac
     9001 tccgagcgct ccacggtttg gtttcagcag agcgcccttc ttgaaagctc gaaaatacgc
     9061 ctccgcgtcc gcgtgccagg gatgggctgg caagtacttg cgcagaatcg ggtataacgg
     9121 atcgccgtgc caaacccagt tcttcaacca atgctgggag gtgacgagca cgaacgccgc
     9181 caggcccact ccgattggaa cccaggagaa cgtcgaggcg ctcttggtcg cggaggcagc
     9241 ggtcgagagc tcacgccagc gggggcgaat acaggcccag attaaaccga gcaggagcgg
     9301 cgcggcggcg caaatcgccg agtacttggt gagagcggcg cccccggcga gcgctccgag
     9361 caaagcggcc ttgaatgaaa gggccttggc tcgcagatct ggcgtcgtcc aaagccgaag
     9421 caccaacaac agccccgcgg gtgcccagat cgcagcgaga tgatcggccc ccgtcgacag
     9481 gttcgagtcg tagacgagga tgccgggata caagaaaaac gcggcccagg accatgacgc
     9541 t
//
LOCUS       DASTKU010000648        11005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1910831, whole genome shotgun sequence.
ACCESSION   DASTKU010000648 DASTKU010000000
VERSION     DASTKU010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11005
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910831"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFQ61_22630"
     CDS             <1..577
                     /locus_tag="VFQ61_22630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957319.1"
                     /translation="KRSTPGLVVLLASVALAFGACSRRADIVDEPDPLLGSGVSAGAT
                     LVEVDAGLADPALPACASREQSGTCSNGGDFPCDFSAWLREYSALCQVESGCKTQGWV
                     AADVADNGCIERVQMSDPNADFIACLGRAARVTRCPCTATTARTFLIPDRSACTATTG
                     AVCGPAEFPCREGEQCIDGTCQAVGGGAGSH"
     gene            767..2782
                     /locus_tag="VFQ61_22635"
     CDS             767..2782
                     /locus_tag="VFQ61_22635"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9957320.1"
                     /translation="MAHTRNDRGPASTLTLPQKLAGLCGTSVLALALVSFVSYRALVE
                     SDGTGRIVAASHAQRAQMDADMMHDALRADVQALRLALDRNDLAAVTNGTLAIRAHSA
                     RLRQDFVRVASNMEPGIQRAVQDARPLVDSYADAVEAVAIAAERSQDLQPKLREFDSS
                     FARLEQQLEQLGDALESCVQDISDQTQLARKAANRRNLLVSGLGGLGLALLSLLFVRS
                     IRAPLLQMARAARHVAEGDVEQRIEYRANDELGRLADALRGMIEYIQSMTSAAEALGR
                     GDLAVQVRARSRKDTLARGFLEMKTNLERSIQDSNRLLQAVQRGDLSARCDPRVFSGV
                     FRDLVEGANGVLEAVSRPFQEAKEVLASVAQRDLSARMRGEYTGDYASIKSSLNEAVT
                     MLEDAMREVIELAVEVANKTQRITSGNTELARTAGAQVQTLGQVTTALSQTTTMSERN
                     AQTVQSSRSHAEDAMKLTEHGANCMERLSCAVEEMKNAADESAKIVRTIDEVAFQTNL
                     LALNAAVEAARAGEAGRGFAVVAEEVRKLAMRSAEAARSTTQVIQRSLQKVEEGVAFN
                     RDASVAFLAISEQIGNITGAMAEIDASSRQQHSGVARLSESVESVMRDARSVADTARN
                     TARDAEALAQRADTMRVSTESFQLASRGGSAKRAELRAVATNRRSVG"
     gene            complement(2834..4033)
                     /locus_tag="VFQ61_22640"
     CDS             complement(2834..4033)
                     /locus_tag="VFQ61_22640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957321.1"
                     /translation="MHIGPLRKRFRHLVGLRAIAPTCWILTLAFATRAAESEVRAAEA
                     ESPESNRGAPPAVPKAPTSEGQPLDSKEQGESPAQSGLLPQPQLPGIGELLPVQLTAF
                     GDIRYALAQDARDRFSIGSLELDASLTLTPYLITTTAIAYDVESNGMALASFTVDGGL
                     FGRDQHHLWRSDWIVRSGVVFGRFDVPFGTAYLEYPSTAARLVTLPASVMATHHAWND
                     IGIQAYAETRYFNVIAYFLNGRDYQATLSDGSPDNRAASFSLGGRVGLLAVPGLEIGS
                     SLALDRHEEGHQDSLFGADLALLSEHFDFRGEYLVAKDGALIRRGSYGRAAFLLDPGF
                     LLARYDVVVEGDAIQSKILDLGAGLKVFKGAEARIVCSRGFEDGTLTGYLQMVGGQSF
                     QPTGFRR"
     gene            4241..4759
                     /locus_tag="VFQ61_22645"
     CDS             4241..4759
                     /locus_tag="VFQ61_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9957322.1"
                     /translation="MMNAITLLKNQHRKVEALFKKLENGRSDAQTVLDELANSLAAHM
                     AIEQEIFYPAVKEVDSDVVNESFEEHSLAEVALKRLLATDPEDEAFDARVTALKELIE
                     HHVEEEEEELFPKVEKALEEEVLTQLAKEMKPRFEEVFEAGFEAAVPKGMAKTSADVS
                     RKKPARKAKSAA"
     gene            complement(4822..6417)
                     /locus_tag="VFQ61_22650"
     CDS             complement(4822..6417)
                     /locus_tag="VFQ61_22650"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9957323.1"
                     /translation="MNSHRLLSSFRLAAVLAIVGGFQDAHAQSAREPLTEQQAVTQLV
                     EHNPGLHSALLQASQAEVLVSAEEGLYSPVLSANAGYTRSRTPYLTGLSGTAVRPSEV
                     VNLGVGLTKPFASGTRLSLDLGAQRTLGTSTTQVPGTTTTGMVTATPVTVLTTSRSYS
                     VTGQLTVTQPLLRGAYNEIGLATLRQARLRRNASVLAAHDTASRSLRDVITAYWELWY
                     AERAVAINQASRDFAREQERTAREQALSGALAQIDALVYSTRLAELEEAVVAALTQRR
                     ARAQALGLLMGDPERAESWTAADPPPGERDAPRDASQAVKAAFENSPELRGYETQIAI
                     ARDARRIAGDALRPRLDLDAYVQAQGLGNGSVPPALEQFGKLQALSAHVGLTFEMPLD
                     DARRKAQVQNAEIETRLTQSDLVAARQRLQSTVLTTLADMESAQQRLRLAIDTQAIAQ
                     RQAEAERQRFRAGASIALQVQQAENSLLQAELRLERARVDLIESQVDMDHLTARLLPR
                     FASVLRSLPQAPAKLDNQAQTPL"
     gene            complement(6410..7615)
                     /locus_tag="VFQ61_22655"
     CDS             complement(6410..7615)
                     /locus_tag="VFQ61_22655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9957324.1"
                     /translation="MFWVTISMALREIRRNSLRSALTMLGVVIGVAAVIALVSVGRGA
                     TQKVTEDIGKLGQNLLMLSPGANVRGGAATPAPPLRVEDAQAIQREILGAAAVAPASS
                     SQQQVVYGNRNTRTQVMGSDNNFFVARSHTIASGRFFTQNEIQSGVSVCVIGQTVREN
                     LFGKASPLGATMRVGRIACQVVGLLTAKGQAGFGQDQDDIVVIPLRAFQRRLSGKIDV
                     NLVFITVAEGRPTSVVADQVRALMRQRRHRSTDSEDDFTVRDMAEIAATVTSTSNAMT
                     ALLGAIAAVSLVVGGIGIMNIMLVSVTERTREIGIRLAIGAFAGEVMLQFLLEAIVLS
                     TLGGVVGILLGLIGSYFATRSMTLPFVVAPDVITLAFVFSALVGVLFGYLPARKAAQL
                     NPIEALRHE"
     gene            complement(7615..8316)
                     /locus_tag="VFQ61_22660"
     CDS             complement(7615..8316)
                     /locus_tag="VFQ61_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9957325.1"
                     /translation="MTAALIELRQVRKIYGSGESEVRALAGVDLDVQRGEFVAIMGAS
                     GSGKSTCMNILGCLDRPSSGHYALSGVAVEDLGGDGLTLLRRHYIGFVFQGFNLLSRT
                     SALENVELPLVYRRIARGERRRRALETLRLVGLSDRADHTPQELSGGQQQRVAIARAL
                     VTDPEILLADEPTGNLDTARTHEIMQLLVELNRERGITIVMVTHEPDVADFAKRVVVF
                     RDGLIVEDRRNPERA"
     gene            complement(8313..9608)
                     /locus_tag="VFQ61_22665"
     CDS             complement(8313..9608)
                     /locus_tag="VFQ61_22665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9957326.1"
                     /translation="MQDRPDGAAILALSKARKRRSLIWRIFLAVVATSALVAVLVFKV
                     GRASGQNYHYLTKPARVGDLRETVTATGTLKGLDSVDVGAQISGRIVRVLVDVNDQVT
                     AGQVLAEIDPEQLRSRVEQSKAQVRAADAAVSLAKATAAQSRLQYERQKDLSQKGLAS
                     QKDLESAQADAERARASVASAEAQASLSRASLKDVQTSLGYTTIRAPMDGVVLARLVE
                     PGQTVAAQLQSPVLFTIARGLAKLELRVDVDEADVGRVKKDLPATFTVDAWPNRKFQS
                     RVVSVYNLPTAGQTVVTYKAVLTVDNQDLSLRPGMTATASIITNERKGVLLVPNAALR
                     FSPPAKVEKSSGPPTLPMMPMRPPRMGAGAGSARPRANAANQGTVWILNAVDPKATPE
                     PSPVSIEVGGSDGENSEMVRGPLKEGQGIIVDVEQRKGS"
     gene            9731..>11005
                     /gene="aspS"
                     /locus_tag="VFQ61_22670"
     CDS             9731..>11005
                     /gene="aspS"
                     /locus_tag="VFQ61_22670"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET9957327.1"
                     /translation="MRANRYRTHTCGELGAAQVGQSVRIAGWVHAIRDHKGIFFFDLR
                     DRYGVTQVVAGEGDSQALRDIARNLTPESVLTVHGVVRPRPEQSVVEAMPTGKIEVVA
                     EQLEVHSLAAPLPFQVRRDDMSNEDLRLKYRFLDLRRQRMQQNIALRARIISFLRNFM
                     DAEGFLDIHTPILANSSPEGARDFLVPSRLHPGMFYALPQAPQQFKQLLMVAGFDRYY
                     QIAPCFRDEDARADRSPGEFYQLDLEMSFAEMEDVFDVCERLFISLTEHFGKKVWKKP
                     FPRFEYEDLLARFGSDKPDLRLGMEIQDLTQDFAQSEFRILKSAAADGRAVRGLCVRN
                     GATMTSSAFKRFEAEAKAQGAKGLLAFGFKKDSPLGMANSERLSEAERKLLADRFGAT
                     TDDLVLMVADKPKAASLVLGAVRRAVGQEFVKT"
BASE COUNT         2022 a         3331 c         3630 g         2022 t
ORIGIN      
        1 gaagcgctcg actcccggac tagtcgttct tttggcctcc gttgcgctgg ccttcggggc
       61 ttgttcccga agggcggaca tcgtcgatga acccgatccg cttttggggt caggcgtttc
      121 cgcaggggcg acgttggtag aggtggatgc aggccttgcc gatccggctt tgccagcctg
      181 cgcgtcacga gagcaatcgg gtacatgctc gaatggcggg gattttccct gcgattttag
      241 cgcttggtta cgcgagtact cggccctatg ccaggtcgag tcgggctgca agacgcaggg
      301 ctgggttgcc gcggacgtcg cggacaacgg ctgtatcgag cgcgttcaga tgagtgatcc
      361 gaacgccgac ttcatcgcat gtctggggcg agccgctcgg gtaacccgtt gcccgtgcac
      421 cgcgacgacg gcacgtactt tcctgattcc cgatcggtct gcgtgtacgg cgacgactgg
      481 ggccgtatgt ggcccggccg agtttccctg tcgcgagggc gagcagtgca tcgacggaac
      541 ttgccaggcc gtgggcggcg gcgcgggttc ccactgacgc cgagacgatg cctggtttgc
      601 gcgcctttcc gagaagtgga agtggcgtca cttgtagaga ggccacgggg gtaacggccg
      661 agctctgacg gatgtggttc ggtgcactac tcattacaaa cttgccacgc gcgaaagtcc
      721 aacctcttag ttgcgcacgg aagaggccgt cttgggatag gcagccttgg ctcacactcg
      781 taacgacagg ggacccgcca gcacactgac attgccccag aagctcgcgg ggttgtgcgg
      841 gacatcggtt ctggctctcg ctctggtcag cttcgtgagt taccgagcgc tggtcgagag
      901 tgacggaacg ggccgcatcg tagcggcaag tcacgcgcaa cgggctcaga tggatgcgga
      961 catgatgcac gacgcgctac gtgcagacgt gcaggcgctg cgtctcgcgt tggatcggaa
     1021 cgacttggcc gcagtcacca acggaacact ggcaatccgt gcccattcgg cgcggcttcg
     1081 acaggacttc gtgcgagtgg catcgaacat ggagcctggt atccagcggg ccgtgcagga
     1141 cgcgcgacca ctggtcgata gctacgcgga tgcggtggag gcggtggcga ttgccgccga
     1201 gcgttcccag gacttgcagc ctaaattgcg agagttcgat tcgtccttcg cgcgactcga
     1261 gcaacagctc gagcagctcg gcgatgctct cgagagttgt gtacaggata tctccgatca
     1321 aactcagctc gcacgcaagg cggcgaatcg tcgaaacttg ttggtgtccg gcttgggcgg
     1381 cttggggttg gccttgctgt cgttgttgtt cgtgcggtcg attcgcgcgc cgctgctcca
     1441 aatggcgagg gcggcgcggc acgttgcaga aggcgacgtg gagcagcgca tcgagtaccg
     1501 cgcgaatgac gagctcggga ggctagcgga cgcgctgcgc ggcatgattg agtacatcca
     1561 gtccatgacc tcggcggccg aggcgctcgg gcgtggggac ctggccgtgc aggtgcgcgc
     1621 gcgttctcgg aaagacacct tggcgcgcgg cttcttggag atgaagacca atctggagcg
     1681 ctcgatccag gacagtaatc ggctgctgca ggcggttcaa cgaggcgact tgtcggcgcg
     1741 ctgcgatccg cgcgtgttca gcggcgtgtt ccgcgatttg gtggagggcg cgaatggcgt
     1801 gctcgaggcg gtgtcccggc cgttccagga ggccaaagag gtgttggcga gcgtcgctca
     1861 acgtgatttg tccgctcgca tgcggggcga atacacggga gactacgcga gcatcaaatc
     1921 ctccctgaat gaagcggtga ccatgctgga ggacgcgatg cgcgaggtca tcgagttagc
     1981 ggtcgaggta gctaacaaga cccagcgcat cacgtcgggt aacacggagc tggcacgaac
     2041 cgctggcgca caggtccaga cccttggtca agtcacgacc gccctctctc agaccacgac
     2101 catgagtgaa cgcaacgcgc agaccgtgca gagttcgcgc agccacgccg aagatgcgat
     2161 gaagctcacc gagcacggag caaactgcat ggagcgcctg tcctgtgccg tcgaagagat
     2221 gaagaacgcc gcggacgaga gcgccaaaat cgttcgtacg atcgatgaag tggcgtttca
     2281 gaccaatttg ctcgcgctca acgcggccgt ggaggcggcg cgagcgggtg aggccggccg
     2341 tggcttcgcc gtcgtagcag aggaggtcag gaagctggcg atgcgtagcg ccgaggcggc
     2401 gcgcagcacc acccaagtga ttcaacgatc cttgcagaaa gtggaagaag gggtggcatt
     2461 caatcgcgac gcgagcgtcg cgttcctcgc gatctcggag cagattggca acatcacggg
     2521 ggccatggcc gagatagatg cctcctcgcg tcagcaacac agcggtgttg ctcggctgtc
     2581 cgagtccgtc gagtccgtga tgcgtgatgc tcgctctgta gcggatacgg ctcgaaacac
     2641 cgctcgtgac gcggaagcgc tggcccagcg ggcggatacg atgcgggtca gcacggaatc
     2701 tttccagctg gcctcccgcg gtggaagcgc gaagcgggct gagttgcgag cggtcgctac
     2761 caatcgacgt tccgtgggct gagcgtcgca tgccggggca gccaacggcc ggctcacgag
     2821 gttcgagcga agttcaacgg cggaatccag ttggctgaaa gctctggcct ccgaccattt
     2881 gcaagtagcc cgtcagggtg ccgtcttcga agcctctgga gcagacgatg cgcgcctcgg
     2941 cccccttgaa taccttgagc cccgcgccga gatccaagat cttgctttga atggcgtcac
     3001 cctccacgac gacgtcgtag cgagccagta ggaatcccgg atcgagtaga aaggcggcac
     3061 gcccgtagga gccgcgccgg atcagggccc cgtccttggc aacgaggtac tcgccccgga
     3121 aatcgaagtg ctccgaaagc agcgcgaggt cggctccaaa caacgagtct tggtgcccct
     3181 cttcgtgtcg gtcgagggcc aacgaactgc caatctcgag gccgggcacc gcgagcaatc
     3241 cgacgcggcc acctagcgag aagcttgccg cacgattatc gggcgaaccg tcggaaaggg
     3301 tggcttggta gtctcgacca ttcaggaagt aggcgatgac gttgaagtag cgggtctcgg
     3361 cgtacgcttg gattcctata tcgttccaag catggtgggt ggccatcaca ctggcgggta
     3421 acgtcaccag gcgtgcggcg gtcgaagggt attccagata tgccgtgcca aagggtacgt
     3481 cgaaccgacc gaacacgacg ccagagcgca caatccaatc cgagcgccaa aggtggtgtt
     3541 ggtcgcgtcc gaatagacca ccatccaccg tgaaactcgc cagcgccatt ccgttggatt
     3601 cgacatcgta agcaatcgcg gtggtcgtga tcaggtaagg cgtcaaggtt agtgaagcat
     3661 cgagttccag ggaacctatg ctgaagcggt cacgggcgtc ttgcgccagt gcgtaacgaa
     3721 tgtcaccaaa agctgtcaat tggaccggca gcaactcgcc tattcccggt agctgaggtt
     3781 gaggcaagag tccggactga gccggggact ccccttgttc cttgctgtcc aagggctggc
     3841 cttcggacgt aggggctttc ggaaccgcag ggggagctcc tcgattcgac tcgggggatt
     3901 ctgcttccgc cgctcggact tcggattcgg cagctcgcgt cgcgaaggcg agcgtcagaa
     3961 tccaacaggt gggcgcaatc gcacgcaacc caactagatg ccgaaagcgc ttacgcagtg
     4021 ggccaatatg catgtattcg catctcttcg aacggcccag tgaggcaaaa gtgaacgtcg
     4081 gctccgttca actgagacgc gcgtggcgaa ctgacgaaac gaggcgttct tacagcgtgt
     4141 ctttcgctag gcggttttgg attcagcctt tcgcggtcgg tgagcgctgt tgttggagga
     4201 tgggctccgc actggcacat gcgttgcagc cgcgaggcgc atgatgaacg caataacctt
     4261 actcaagaac caacaccgca aagtcgaggc gctattcaag aagctcgaga atgggaggtc
     4321 tgacgcacaa accgttctgg atgagctggc aaacagcctc gccgctcaca tggcaatcga
     4381 acaggagatt ttctatcccg ccgtcaaaga ggtggactcc gacgtcgtca acgagagctt
     4441 cgaggagcac tccctggcgg aagttgcgct gaagcgactg ctcgcgacgg atccggaaga
     4501 cgaagccttc gacgcgcgag tgaccgcgct caaggagttg atcgagcacc acgtcgaaga
     4561 ggaagaggag gagctctttc cgaaggtgga aaaggcgctc gaggaagaag tgttgaccca
     4621 gctcgccaag gaaatgaagc cgcgcttcga ggaagttttc gaggcgggtt tcgaggctgc
     4681 agttccgaag ggaatggcca agacttcggc ggacgtttca cgcaaaaagc cggcacgcaa
     4741 ggccaagtcc gccgcgtgaa gcggtcgaat cagggcgcga tcacgaccgt acacggctgg
     4801 ccaagcgcca agacccgctg actacagcgg ggtctgcgct tggttgtcga gcttggctgg
     4861 agcctgcggt agggaccgaa gcaccgaagc gaagcgcggc aggagccgag ccgtgaggtg
     4921 atccatgtcc acctgactct cgatcaggtc gacccgagct cgctccagtc gcaactcggc
     4981 ttgcaacaac gagttttcgg cctgttgcac ctgcagcgcg atcgaggcgc cagctcggaa
     5041 gcgttgacgt tccgcctcgg cctgcctctg cgcaatggct tgggtatcga tcgcgaggcg
     5101 tagacgctgc tgggcgcttt ccatgtcggc caacgtcgtg agcaccgtgc tctgcaggcg
     5161 ctggcgggcg gccacgagat cgctctgagt gaggcgcgtc tcgatttccg cattttgaac
     5221 ctgggccttt cgtcgagcgt cgtcgagcgg catttcgaat gttagcccga cgtgagcgct
     5281 caaggcctgt agctttccga actgttcgag tgcgggtggg acggagccat tgccgagtcc
     5341 ttgcgcttgc acataagcgt ctagatccag tcggggtcgg agtgcgtctc cggctatgcg
     5401 gcgcgcatct cgggcgatgg caatctgggt ttcgtagccc cgaagttcgg gtgagttctc
     5461 gaacgctgcc ttcaccgcct gggatgcgtc gcgcggcgca tcgcgttcgc ccggcggggg
     5521 atctgcggcc gtccagcttt cggcgcgctc cggatcgccc atcagcaagc ccaaagcctg
     5581 ggcgcgcgcg cgccgttggg tgagtgcggc cacgactgcc tcctcgagtt ctgcgaggcg
     5641 cgtgctgtag acgagcgcgt caatctgggc gagcgcgccg ctcagggctt gttcccgcgc
     5701 agtgcgttcc tgctcgcgcg caaagtcgcg tgaagcttgg ttgatggcga ccgctcgttc
     5761 tgcgtaccag agctcccaat acgccgtgat tacgtcgcgc aggctgcggc tggccgtgtc
     5821 atgagccgcc agcacactcg cgttccgacg cagccgcgcc tggcgcagcg tggccaaacc
     5881 gatctcgttg tacgcgccgc gtagcaacgg ctgcgtcacc gttagctgcc ccgtcaccga
     5941 atagctgcgg ctggtcgtca acacggtcac cggggtcgcg gtcaccatgc ccgtggtggt
     6001 ggttccgggg acttgcgtgg tcgaggtccc gagcgtccgt tgcgccccga gatccaagct
     6061 gagccgcgtt ccgctcgcga acggcttagt caagccgacg cctagattca ccacctcgct
     6121 gggtcggact gcagtaccag aaagaccggt cagatacggc gtgcgcgagc gcgtgtagcc
     6181 cgcgttcgcg ctcaacacgg ggctatacaa accctcttcc gcgctgacca gcacttcggc
     6241 ctggctggct tgcaacagcg cgctgtgcaa ccccggattg tgctccacga gttgggtgac
     6301 cgcctgctgc tcggtcaaag gttcgcgagc gctctgggcg tgtgcgtcct ggaacccgcc
     6361 aacgatggcg agtacagcgg cgagacggaa tgagctcagc agacggtggc tattcatggc
     6421 gcagggcttc gattgggttc agttgggcgg ctttgcgcgc gggcagataa ccgaacaaga
     6481 cacccaccaa ggcggagaac acgaaggcca aggtgatcac gtcgggcgcc acgacgaatg
     6541 gcagcgtcat cgagcgggtc gcgaagtacg agccgatcag gccgagtaga atgcccacca
     6601 cgccgcccag cgtggagagc acgatagcct cgagcaagaa ctgcagcatc acctcgcccg
     6661 cgaatgcccc gattgcgagg cgaatgccga tttcccgggt tcgctcggtc acggacacga
     6721 gcatgatatt catgatgcca atcccgccca cgaccaagct gacggcggcg atggctccga
     6781 gcagcgccgt cattgcattc gacgtgctgg tcacggtggc cgcaatttct gccatgtcgc
     6841 gtacggtgaa gtcgtcttcc gaatcggtgg agcgatgccg gcgctggcgc atcagggcgc
     6901 ggacctggtc cgccacgacc gaagtgggtc ggccttcggc aacggtgatg aacaccaagt
     6961 tgacgtcgat ctttccgctc agccggcgtt gaaacgcgcg cagcgggatc acgacgatat
     7021 cgtcctggtc ttgaccgaag ccggcctggc ctttggccgt gagtaaccct accacctggc
     7081 acgcaatgcg tccgacgcgc atggtggccc cgagcgggct cgctttacca aacaagtttt
     7141 cgcgcacggt ctgcccgatc acgcagacgg agacgcccga ctggatctcg ttctgcgtaa
     7201 agaaccggcc cgaggcaatg gtgtggctcc gcgcgacgaa gaagttgttg tccgagccca
     7261 tcacctgtgt gcgcgtgttg cggttgccgt acacgacttg ctgctgagag ctcgatgccg
     7321 gggccaccgc cgcggcgcct aggatctcgc gctgaatagc ctgcgcgtct tccacacgga
     7381 gtgggggggc gggagtcgcg gctccgccgc gcacgttcgc gccggggctc agcatcaaca
     7441 ggttctgccc gagcttgccg atatcctcgg tcaccttctg ggtcgcgcca cgccccacgg
     7501 acacgagcgc gatcaccgcc gcgacaccga tcacgacgcc gagcatggtg agcgcagagc
     7561 gcagagagtt ccggcgaatc tcgcgtaggg ccatgctgat ggtgacccaa aacattaggc
     7621 gcgctccggg tttcgacggt cctcgacgat caggccgtcg cgaaacacca ccacgcgctt
     7681 cgcgaagtcg gcgacgtcgg gttcgtgggt gaccatgacg atggtaatgc cgcgctcacg
     7741 gttcagctcg acgagcagct gcatgatctc gtgggttcga gccgtgtcga ggttgccggt
     7801 gggctcgtcc gccaacagga tttccggatc cgtgacgagc gcgcgtgcga tcgcgacacg
     7861 ctgctgctgg ccgcccgaga gctcttgagg cgtgtgatcc gcgcggtcgg acaggcccac
     7921 caagcgcagc gtctctagag cccgccgacg ccgttcgcca cgcgcgatac gacgataaac
     7981 gagcggcagc tcgacgttct cgagcgcgct agtgcgcgag agcaggttga aaccctggaa
     8041 aacgaagccg atatagtgcc ggcgcagtag cgtgaggccg tcgccgccca agtcctccac
     8101 cgccacgcca gacaaagcgt agtggccgga actcggacgg tccaagcagc caaggatgtt
     8161 catgcaggtg gacttgccgg aaccactcgc gcccatgatg gccacgaact caccgcgctg
     8221 cacgtccagg tccaccccgg ccagggctcg aacctcggac tcgccggaac catagatctt
     8281 ccggacttgg cggagctcga tcagagccgc ggtcatgaac ctttgcgctg ctccacgtcg
     8341 acgatgatgc cttggccctc tttcagtggg cctcgcacca tctcgctatt ctcgccgtcc
     8401 gacccgccca cttcgatgct gaccgggctg ggctcgggag tagccttggg atcgacggca
     8461 ttcaggatcc agaccgtacc ttggtttgcg gcgttggcgc gcggccgggc gctgcctgcg
     8521 cccgccccca tgcgcggcgg acgcatcggc atcatgggca gggttggagg cccggagctc
     8581 ttctctacct tggcgggcgg agaaaaccgc aatgcggcgt tcgggaccaa gagtacgcct
     8641 ttgcgttcgt tggtaatgat cgaggcggtc gccgtcatcc cggggcgcag agacaagtct
     8701 tggttgtcga cggtgagtac cgccttgtaa gtgacgaccg tttgccccgc cgtgggcagg
     8761 ttgtagacgg agacgacgcg cgactggaat ttgcggttgg gccaggcgtc cacggtgaag
     8821 gtcgcgggca aatccttttt cacccggccg acgtcggcct cgtcgacgtc cactctgagt
     8881 tcgagcttgg cgagcccgcg cgcgatcgtg aacaacacgg gggactgcag ttgggccgcg
     8941 acggtttgtc cgggctccac caagcgcgcc agcaccacgc cgtccatggg agcgcgaatg
     9001 gtggtgtagc cgagggacgt ctgcacatcc ttgagcgagg ctcgcgacag gctggcttgc
     9061 gcttcggccg acgccacgct ggcccgggcg cgctcggcat ccgcctgtgc ggactcgagg
     9121 tccttctggg aagccaggcc tttttggctc aaatccttct gtcgttcgta ttgcaaacga
     9181 gattgagccg cggtggcttt ggccaagctc accgcagcgt ctgccgctcg gacttgcgct
     9241 ttggactgtt cgacgcggga tcgaagctgc tccgggtcaa tctcggccaa tacttggccc
     9301 gcggtgacct ggtcattgac gtcgaccaga acgcggacga tgcgcccgga gatctgcgcg
     9361 ccgacgtcca ccgagtccag gcccttcaag gtcccggtcg cggtaacggt ttcccgcagg
     9421 tcccccacgc gcgcgggttt cgtgaggtag tgataattct gcccgctggc tcgtccgact
     9481 ttgaagacga gcaccgcgac tagggcagac gtggcaacta ctgccagaaa gatgcgccag
     9541 atcaggctcc tacgtttgcg tgccttcgag agagccaaga tggcggcacc atcgggtcgg
     9601 tcctgcatga gcttagagcg cagtgtatcc gagaatatgc agcctgcctg gcttcaaggg
     9661 ctgcttcctt ggcgccacgg tgtgtgctag cgtgcccggc ccgtttaccg aaaaacacgg
     9721 aactttcgcc atgcgcgcca atcgatatcg aacccacacc tgcggcgaac tcggcgcagc
     9781 acaggtgggg cagtcagtta gaatcgcggg ctgggtccac gccatccgcg accataaagg
     9841 tattttcttc ttcgatctgc gtgatcgcta cggcgttacg caggtggtcg ctggggaagg
     9901 ggactcgcaa gcgctgcgtg atatcgcgcg caacctcact ccagagtctg ttttgacggt
     9961 gcacggagtc gtgcgtccgc ggccggagca gagcgtggtc gaggccatgc ccaccggcaa
    10021 gatcgaggtg gtcgcggagc agctcgaggt gcattcgctg gccgctccat tgcccttcca
    10081 ggtccggcgc gacgacatgt cgaacgagga tctacgcctg aagtaccgct ttttggatct
    10141 gcgccggcaa cgcatgcagc agaacatcgc gctgcgagcc cggatcatca gcttcctgcg
    10201 caacttcatg gatgcggagg gcttcctcga tattcacacg ccgatccttg cgaactcgtc
    10261 gcccgaaggc gcgcgcgact tcttggttcc gagccgcttg caccccggca tgttctacgc
    10321 gctcccgcag gcgccccagc agttcaagca gctgctgatg gtggccggct tcgaccgcta
    10381 ctaccaaatt gctccctgct ttcgagacga agacgcgcgt gcggatcgca gcccgggcga
    10441 gttctaccaa ctcgatctcg agatgtcgtt cgcggagatg gaggacgtgt tcgacgtctg
    10501 cgagcgcctc ttcatttccc tcaccgagca cttcggcaag aaggtctgga agaagccgtt
    10561 cccgcgcttc gagtacgagg acctgttggc gcgattcggc tctgacaagc ccgatctgcg
    10621 gctcggcatg gagatccagg atttgactca ggacttcgcc cagtcggagt tccgaatcct
    10681 gaagtctgcc gcggccgatg ggcgcgccgt tcgcgggctt tgcgtgagga acggcgcgac
    10741 catgacatcc tcggcgttca agcgtttcga ggcggaagcc aaggcgcaag gcgccaaagg
    10801 cctgctggct ttcgggttca agaaggacag cccgctcggg atggcgaaca gcgagcgcct
    10861 gagtgaggcg gagcgcaagc tgctcgccga ccgcttcggg gccacgaccg atgatctggt
    10921 gctgatggtc gcggacaagc ccaaagcggc ctcgctcgtg ctcggcgctg tgcgtcgcgc
    10981 agtggggcaa gagttcgtga agacc
//
LOCUS       DASTKU010000649         3979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_347006, whole genome shotgun sequence.
ACCESSION   DASTKU010000649 DASTKU010000000
VERSION     DASTKU010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347006"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..754
                     /locus_tag="VFQ61_22675"
     CDS             224..754
                     /locus_tag="VFQ61_22675"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9957328.1"
                     /translation="MAKLVFGMNQSLDGYVDHTEFGPSATLFRHFIKEAEGQAGSVYG
                     RKLYELMRYWDDDQAEWGEPERAFAAAWRKQPKYVVSRSLKSVGPNATLIPGDLEGAI
                     RKLKAEHEGEFEIGGPNLAKTLTELGLIDEYRIYLHPVVLGKGTPYFGGPRPRLRLVA
                     YDQIDETVLRLKYVPA"
     gene            complement(768..1463)
                     /locus_tag="VFQ61_22680"
     CDS             complement(768..1463)
                     /locus_tag="VFQ61_22680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957329.1"
                     /translation="MNSAKKLALVLGLFLVGCARDAPSETPPTQEKQVPLLVGEPCAL
                     SSAPQVSSKPPRIFVELATLEGDLTAIQQTNTAAMKSEAAPRTFSQMLADPRWKAISV
                     RHVLASDGTRQTFPWEFGPPLASRECPASERWVLSLTPHVTGQSPVMVRVDIQIVPAP
                     SPHDVPSASPVPPECGARTTLLVRDQQVVVLSGFPTSTPGRAGLMTTVTPYVIWEDAD
                     LRRLGECKAKRAH"
     gene            complement(1632..3356)
                     /locus_tag="VFQ61_22685"
     CDS             complement(1632..3356)
                     /locus_tag="VFQ61_22685"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HET9957330.1"
                     /translation="MIWQPTSTTEEAGFLAALEEAPDSREIRRAYADWLMQRDDLRGR
                     WLEAHEELWAESLKPRRCRELQPLLRKLARECDPQWLMRVARISFSEMRRRVQVLANL
                     DPARKLFAAGSHRYELKPPLELEQLARLEAGLGLRLPEQYRRFVTEFANGGAGPAYGI
                     VPLAKTLPSASRASRLNEPFPVPKSAEDSPGYGAPPGALEVCETGCGGYYWLITAGPD
                     REVLWQQGSEGDWWPLVANGHAPEDQLLHFDAPRAARLEFIDWYINWLDDARCELLRA
                     MPDGDDVFDVAPEGVTDLNFSGRGLTSLPDGVRKLRYLRKLNLNDNKLTALPDWLGEL
                     THLEELHIAGNSLKTLPASIGELHALTRIRAQHNQLESLPETLGQLRALNTLDLSYNR
                     LTALPESIGDLVELDYIRINSNLLSTLPASFGRLVKVECLDLSFNQLTTLPAELSALH
                     ELSELNLRKNALRLLPEAIGQLPKLERLILNENPELDLADACRKLSHASTLRELQLIN
                     LGLDALPDEIGLLGQLVSLAVSYNRISRLPGTFANLTRLRDFSDRSNPADLDGLAREL
                     IPGLNQDE"
     gene            complement(3353..3532)
                     /locus_tag="VFQ61_22690"
     CDS             complement(3353..3532)
                     /locus_tag="VFQ61_22690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957331.1"
                     /translation="MANSRLSAGLVGRQTEVGDLHWLGAIPNLLAPAHAQRKINHFGS
                     SDVQSATLRYTLETR"
     gene            3531..>3979
                     /locus_tag="VFQ61_22695"
     CDS             3531..>3979
                     /locus_tag="VFQ61_22695"
                     /inference="COORDINATES: protein motif:HMM:NF025466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4275 family protein"
                     /protein_id="HET9957332.1"
                     /translation="MCAFLMTSATPFEFALLNGAVVTVLDLHQRRRLMCMWREKFASS
                     VMRQTGRSVHLGFDWHAFSYGFTRSKHGLRGLELYLAEAAPEVYVVPEDEDNPAFACR
                     ASALLDFSSCHTDVLVFPVALSWTMAFTHEQPELGPYFSRAEWCELDP"
BASE COUNT          748 a         1189 c         1249 g          793 t
ORIGIN      
        1 cctttggacc cgtagttagg gtgtgtctga cggaacgctt gcggtgtatg caagcgtatg
       61 gcacgcaacg acccagtcag atcgatcggg cagcccgagc gtggggcgtt catgcgtact
      121 ccaaaaacca gttgcgatat acgatcagta cgtctagctt acaaccagtt gtagatcgca
      181 atcagttctt gcggcgaaag tctcgctatg gaagggcact ggaatggcta agctcgtgtt
      241 cggaatgaac cagtcgctag acggctacgt cgaccatacg gagtttgggc cgagcgcaac
      301 gctgttccgc cacttcatca aagaggcgga ggggcaggcg ggaagtgtgt acggccgcaa
      361 gctgtatgag ctgatgcgtt actgggacga cgatcaggcc gaatggggtg agccggaaag
      421 ggccttcgca gcggcgtggc gaaagcagcc gaagtacgtc gtctcgcgct cgttgaagtc
      481 tgtgggtccc aacgccacct taataccagg ggacctcgag ggagcgatcc gcaaactgaa
      541 ggccgagcac gagggtgagt tcgaaatcgg cggtccgaat ctggcaaaaa cgcttaccga
      601 gctcggtctg atcgatgaat atcgaatcta cctgcacccc gtggtgctgg gtaagggtac
      661 accgtatttc ggcggccccc ggccacgttt gcgcctcgtt gcgtatgacc agatcgacga
      721 gactgtgctc cggttgaagt acgttccggc gtgagatctt gtctacgtca gtgagcgcgc
      781 ttggccttgc attcgccgag gcgtcgtaga tccgcgtcct cccagatgac gtatggagta
      841 acagtggtca tgagcccggc tctccccggc gttgaagttg gaaagccgga gagaacgacg
      901 acttgctggt cacgcacgag caaggtagtc cgggcgccgc attccggcgg gacaggcgat
      961 gcgctgggga cgtcgtgggg cgacggggca gggacgatct gtatatctac gcgcaccatc
     1021 acgggagatt gtccagtgac gtgaggtgtc aagctgagga cccaacgttc actcgcgggg
     1081 cattccctgg aagcgagcgg cggcccaaac tcccaaggga acgtttggcg agtcccatcg
     1141 ctcgcgagca catgacgcac gctgatcgcc ttccagcgcg gatccgcaag catctgtgag
     1201 aaggtgcgcg gcgctgcttc gcttttcata gcagccgtgt tcgtctgttg aatcgccgtc
     1261 agatcgcctt cgagcgtggc aagctccacg aagatccgcg gcggtttcga tgacacttgt
     1321 ggcgcagaag aaagcgcaca cggttcgccg accaagagtg gcacctgctt ctcttgggtg
     1381 ggcggcgtct cggagggtgc atcgcgcgcg cagccgacca gaaaaagccc caggaccaaa
     1441 gccagcttct ttgcactatt caattcgctt ctccgagatt gaaatccgac cgcccaactt
     1501 tccaccgacc atcgccgccc acaggcgttt cgcccagccg ttcacctttc atcggtccaa
     1561 tgaacgccga tcaccggcaa cgcattccgg ccgctgctct cggcggcgcc gcgcctctca
     1621 tgtgcgactc actactcatc ttgattgagg ccagggatga gctcccgcgc gagcccgtcg
     1681 agatccgcag ggttcgatcg gtcactgaag tcgcgcagtc gggtgaggtt cgcgaacgta
     1741 cccggcagcc gagaaatccg gttgtaactc acggccaagg acacgagttg ccccagtagg
     1801 ccgatttcgt ccggcagcgc atcgagcccc aagttgatca gctgcagttc tcgtagcgtg
     1861 gaggcgtgtg acaacttacg gcaagcatcg gcgaggtcga gctccgggtt ttcgttgagg
     1921 atgagccgtt cgagtttggg caactgcccg atcgcctccg gaagcaggcg cagcgcgttc
     1981 ttgcgcaggt tgagctcgct gagctcgtgc agcgcactca gctcggccgg tagagtcgtc
     2041 agctgattga aggacaaatc gaggcattca accttcacga gccgtccgaa gctcgccggc
     2101 aaggtgctca acagattcga gttgatccga atatagtcga gctcgaccaa atcaccgatg
     2161 ctctcgggca aagcggtgag gcgattgtag gaaaggtcga gcgtgttgag tgcgcgcagc
     2221 tgtcccaggg tctcagggag gctctccagt tgattgtgct gggctcggat acgggtcaag
     2281 gcatgcaact cgccgatgct ggcgggcagc gtcttcaagc tgtttcccgc gatgtgcagt
     2341 tcctccaagt gcgtgagttc accgagccag tccggtagcg ccgtgagctt gttgtcgttg
     2401 aggttgagct tgcgcaggta gcggagcttc ctcacgccgt cgggcagcga cgtcagcccg
     2461 cggcctgaga agttgagatc agtgacgcct tcgggcgcga catcgaacac atcgtcgccg
     2521 tcgggcattg cacgtaggag ctcgcagcgt gcgtcgtcga gccagttgat gtaccaatcg
     2581 atgaactcca gccgagccgc gcggggggcg tcgaagtgaa gtaattggtc ctctggggcg
     2641 tgtccgttcg caaccaatgg ccaccagtcc ccctcggagc cttgctgcca gagcacctcg
     2701 cgatcgggcc ccgcggtgat caaccagtag tatccaccac aaccggtctc gcagacctcg
     2761 agcgccccgg gtggcgcgcc gtagccgggc gaatcctccg cgctcttcgg aacggggaag
     2821 ggctcgttca gtcgagatgc gcggctcgcg gatggaagtg tcttcgcgag cggtacgatt
     2881 ccgtaggcgg ggccggctcc gccgttggca aactcggtga cgaagcgccg atactgttcg
     2941 ggcaggcgca acccgagacc cgcctccaaa cgtgcgagct gctccagttc gagtggtggc
     3001 ttcagctcgt atcggtggct tccggccgcg aagagcttgc gcgcgggatc caggtttgca
     3061 agcacctgca cacgccgccg catctccgaa aagctgatac gcgccacgcg catcagccat
     3121 tgagggtcgc attctcgagc gagtttccgc aggaggggct gcagctcgcg gcagcggcga
     3181 ggcttcagcg actccgccca cagctcttca tgtgcctcga gccaccttcc gcgcaggtcg
     3241 tcgcgttgca tgagccagtc ggcgtaagcc ctgcggatct cgcggctgtc gggcgcctct
     3301 tcaagggcgg ccagaaaacc ggcctcttcg gtggtgctgg taggctgcca aatcatcgcg
     3361 tctcgagcgt atagcgcaag gtcgcgctct gcacgtcgct ggacccgaaa tgattgatct
     3421 tgcgctgggc gtgggctggt gcgagtaaat tcggaatcgc tcctagccaa tggagatccc
     3481 cgacctcagt ctgacgacct accaatcccg cgctcagtcg actgttcgcc atgtgtgcat
     3541 tcctcatgac ctcagcgaca ccctttgaat tcgcactgct gaacggagct gttgtcaccg
     3601 ttctcgactt gcatcagcgt cgacgcttga tgtgcatgtg gcgcgagaag tttgctagct
     3661 ccgtcatgcg ccagaccgga aggtcggttc acttgggctt cgattggcat gcgttcagct
     3721 acgggttcac tcgctctaaa cacggactgc gcggacttga actctatctc gccgaggccg
     3781 cacccgaggt ctatgtcgtc ccggaagatg aggacaaccc cgcctttgcc tgccgggcgt
     3841 ccgcgcttct cgacttctcc agttgccaca cggatgtcct cgttttcccg gtcgccttga
     3901 gctggactat ggctttcacc cacgagcaac cggagctcgg cccgtacttc tcacgggctg
     3961 aatggtgtga gctcgaccc
//
LOCUS       DASTKU010000650         3735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1910957, whole genome shotgun sequence.
ACCESSION   DASTKU010000650 DASTKU010000000
VERSION     DASTKU010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1910957"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VFQ61_22700"
     CDS             complement(<1..776)
                     /locus_tag="VFQ61_22700"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957333.1"
                     /translation="MARRDPHSFADEEQARTASFALDAHVDFQTRTIAAQVTLTFEAP
                     ARGRLDLDTRDLSIESVADATGRALDFTLHPAEPHLGSRLEIEVPEPTPRIVIRYHTS
                     PEASALQWLEPAQTAGRVHPYLFTQCQAIHARSLVPLQDTPSIRVTYSAQLRFPRQLR
                     CVMAAADRGREEPDSEPTQAVHRWEMPQPIPPYLFAFAIGDLASRELSLRSRVWAEPS
                     VVDAAAWEFAVVEDHIRAAEALFGPYDWERFDILIMPPSFP"
     gene            complement(817..1647)
                     /locus_tag="VFQ61_22705"
     CDS             complement(817..1647)
                     /locus_tag="VFQ61_22705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957334.1"
                     /translation="MNFRSVLGLGKRISACSRNRELESTNNFVTWLAPKLRICSLLTA
                     LALGTCATWLTVAPESIERRLRPHVIQKLRDEARVRFPALDSWSRWVEGAQSASAHYR
                     RRLDSGEPEKLARLLAPVLDSLCELDCGEGAIASTIREGFAESIRSWDLVSARLRSWA
                     RGRYSELISQVVADVRVFLWTNAGLLGLAAGILYWRGAGRVAVLFSGVLLVTVAIGAY
                     LYVFGQDWLFTLLFARYLGYGYLGWCALIAVALLDCAFNRGRIVSALLGATTRAIAPT
                     "
     gene            1764..3470
                     /locus_tag="VFQ61_22710"
     CDS             1764..3470
                     /locus_tag="VFQ61_22710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957335.1"
                     /translation="MQVAYSKYLVHRSSFMALTAVAALSLAACSSTSPSPLGTGGMAG
                     SVVGVSGAGSGGTSVPNTGAGGTTSSTQSSPTGSGGVSPTNGGSPAQGGAAAGGAAAG
                     GAVTAGMGGAFVAGATGMPTAGAASGAGGGAPGGSGGSAGAGTGGAHPMDHCLYGYDP
                     EPTDATMKDGPAEFFPVGKTDPNIVDTTLQPEVISWMEKNRWQEAHVEWHAIRGCSVP
                     GGGGLSKVNICSYKQLVPKDQNCQTEGDGYQFLMFHRHMMIALKQLWPKHTEQFEGFK
                     KFPTSAEDVPPQWRSQWKNWDADVLAAGKLGDEIDKPENLAKFPSEGALGFWLQCNVG
                     QKLASVPNMPFVGLHFVLHAKWARPGNTRHGVNNTDSNIDNYMFYKLHGWIDNVWEKY
                     RRAKGLTQEDAKYKADLLAQCREMDSEIEIIKKTKPLPTDETTEPLPVESGDFHTKVR
                     PIFEKPDNLCAGCHAETGSNAGLILGGNISSKKIVEGLVNKPSRDGGQFKLVAPGDPD
                     HSWLYLKAAGKAEGAGCTPASTAQCITGVMPQNASGLPTVTAAELQTLRQWILDGAKG
                     PT"
     gene            3516..>3735
                     /locus_tag="VFQ61_22715"
     CDS             3516..>3735
                     /locus_tag="VFQ61_22715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957336.1"
                     /translation="MRRPAWLGPDAPPAQYEDIAPARARSRCARPEALKAMLAPPRRC
                     AWPRVLSARLPAGPRIVTMGTVPTYVYET"
BASE COUNT          799 a         1171 c         1148 g          617 t
ORIGIN      
        1 gggaacgaag gtggcatgat cagaatgtcg aagcgctccc aatcataggg accgaacaac
       61 gcctccgccg cgcgaatatg gtcctctacc accgcaaact cccaggcggc ggcgtccacg
      121 acgctcggct ccgcccacac ccgggatcgc agggacagct cgcgggacgc caaatctcca
      181 atcgcaaacg cgaacaggta cggtgggatg ggctgaggca tctcccagcg atggaccgcc
      241 tgggttggct cggagtctgg ctcttctcgt ccgcgatccg ccgccgccat tacgcagcgc
      301 aactgacgcg gaaagcgcag ttgcgcggaa taggtaacac ggatactcgg cgtgtcctgc
      361 agaggcacta aggagcgcgc gtggatggct tggcactgag tgaacaagta cgggtgcacg
      421 cggcccgcgg tttgcgccgg ctcgagccac tgcaacgcgg atgcctcggg cgacgtgtgg
      481 taacggatga caatacgggg agttggttcc ggaacctcga tctcgaggcg cgaaccgagg
      541 tgcggttctg caggatgtag agtgaaatcc agcgcacggc cggttgcatc cgcgacggat
      601 tcgatcgaga gatcgcgggt atcgagatcc agtcgccctc gtgcgggcgc ttcgaaggtc
      661 aaggtgacct gagccgcaat cgtccgcgtc tggaagtcga cgtgggcatc gagtgcgaaa
      721 cttgcggtgc gagcttgctc ttcgtcggcg aatgaatgcg gatcgcggcg ggccatggcc
      781 cggcagcata gctcggcgcc agtgaataac ccagcttcac gtgggagcga tggctctagt
      841 cgtggctccg agcaacgcgc ttacgatgcg cccgcggttg aacgcgcaat cgagcaaagc
      901 gactgcgatc agcgcgcacc agccgagata gccatatccc aggtagcgag caaacagcag
      961 cgtgaacaac caatcctggc caaagacata caggtaggcg ccgatcgcaa ccgtgaccag
     1021 caacaccccg ctgaacagca ccgcgacccg ccctgctcca cgccaataca atatgccggc
     1081 cgccagcccg aggagacctg cgttcgtcca aagaaacaca cgtacatccg ccacgacctg
     1141 tgaaatcaac tcagagtacc gaccccgagc ccaagacctc agtcgggcgc tcaccaaatc
     1201 ccaagatcgg atgctctccg cgaacccctc gcggatcgtc gacgcgattg ctccttctcc
     1261 acagtcgagc tcacagagcg agtcgagtac gggcgctagc aatctcgcca atttctccgg
     1321 ctcgcctgaa tccagccgtc gccgatagtg cgcagaagcc gactgcgcgc cctcgaccca
     1381 gcgcgaccac gagtcgagtg cagggaagcg cacgcgcgct tcgtcgcgca acttttgaat
     1441 gacatgaggg cgcaagcgcc gctcgatgga ctccggagcg acggtcagcc aggttgcgca
     1501 agtcccgagc gccagcgccg tcagcaacga gcagattcgc agtttcgggg cgagccacgt
     1561 gacgaagttg ttagtgctct ccagctctct attacgcgag caggcgctga tgcgcttacc
     1621 caacccaagt acgcttctaa aattcacgag actcgaatat cgcgctgaga ttcgaaaatt
     1681 cccgcttgga ccgagctttt ttgcgaaagt tgcgttaagg gcaattcaac tcggggccat
     1741 gcgggcatgc acccgtgagg accatgcaag tcgcctactc caagtacctt gtccatcgca
     1801 gttccttcat ggcgctcacg gctgtagcag ctttgtcact tgctgcgtgc agtagcacca
     1861 gtccgagccc gctgggaact ggcggcatgg ccggaagcgt cgtgggcgta tccggtgcag
     1921 gttcgggcgg aacctctgtt ccaaacaccg gagcaggtgg aaccacgagc tccacgcaga
     1981 gcagtccgac cggcagcggt ggggtctccc cgacaaacgg cggatcaccg gcgcaaggtg
     2041 gtgcagccgc gggtggcgcg gctgctggtg gcgcagtcac cgccggtatg ggtggtgcgt
     2101 tcgtcgcggg agcgacgggc atgcccactg cgggcgccgc cagcggagcg ggtggcgggg
     2161 ctcccggtgg cagcggagga tctgctggcg cgggcaccgg cggtgcgcat cccatggatc
     2221 actgtttgta tgggtacgat cccgagccca ccgacgcgac catgaaagat ggaccagccg
     2281 agttctttcc ggttggcaaa accgatccca atatcgtgga cacaacactc cagcccgaag
     2341 tcatcagctg gatggagaag aatcgctggc aagaggcgca cgtcgagtgg catgcgattc
     2401 gcggctgctc ggtcccgggc ggcggcgggc tcagcaaagt gaatatctgc agctacaaac
     2461 agctggtgcc caaagatcag aattgccaga cggagggtga cggttaccaa ttcttgatgt
     2521 tccaccggca catgatgatt gccttgaagc agctgtggcc caagcacacc gagcaattcg
     2581 agggcttcaa gaaattccct acctcggccg aagacgttcc gccacaatgg cgatcacaat
     2641 ggaagaactg ggacgcggac gtgctcgcgg ctggcaagct tggcgatgaa atcgataagc
     2701 cggagaacct cgcgaagttc ccgtccgaag gcgccctcgg cttctggctg caatgcaacg
     2761 tcggtcagaa gctcgcgagc gtccccaaca tgccgttcgt gggtctgcac ttcgtgcttc
     2821 acgccaagtg ggcgcgcccc ggcaatacgc gacacggagt caacaacacc gactccaata
     2881 tcgataatta catgttttac aaattgcacg gctggatcga caacgtgtgg gagaagtatc
     2941 gtcgtgcaaa gggcctgacg caggaagatg ccaaatacaa ggcggacctg ctcgcgcagt
     3001 gtcgcgagat ggattcggaa atcgagatca tcaagaagac caagcctttg ccgaccgacg
     3061 agaccacgga gccgctgccg gtcgagagtg gtgatttcca cacgaaggta cgccccatct
     3121 tcgaaaagcc agacaacctg tgcgccggct gccacgcgga aacgggctcc aacgctgggc
     3181 tcatcttggg gggcaacatc agctccaaga aaatcgtcga aggcctggta aacaagccct
     3241 ctcgcgacgg tggtcaattc aaactcgtcg cccctggcga tccagatcac agctggctct
     3301 acttgaaagc agcgggcaaa gccgaaggcg caggttgcac gccggccagc accgctcaat
     3361 gcattacggg cgtcatgcct cagaacgcca gcggcctgcc taccgtcacc gcggccgagc
     3421 tccaaacctt acgtcaatgg atcctcgacg gggccaaggg cccaacctga acgcccgagc
     3481 gcaccaatcc ctcccgaata tggtatttgg tggcgttgcg cagaccagca tggctcggac
     3541 cagatgctcc gccggcgcaa tacgaagata tcgcgccggc ccgtgctcgc tcccgctgcg
     3601 cgcggcccga ggccctcaag gccatgctcg cgccgccgcg gcgctgcgcg tggccgaggg
     3661 tcctttcggc gcgcttgcca gcgggccctc gcatcgtcac catggggacc gtgcccacgt
     3721 acgtttacga aaccc
//
LOCUS       DASTKU010000651         5820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_347171, whole genome shotgun sequence.
ACCESSION   DASTKU010000651 DASTKU010000000
VERSION     DASTKU010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347171"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFQ61_22720"
     CDS             complement(<1..272)
                     /locus_tag="VFQ61_22720"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957337.1"
                     /translation="MQSFTQISRGLAEIAGLLEFAGEPKFKVKAYANAAEVVDTLGDE
                     LASLVEQNRLQELPGIGRAIEQQIQELWNTGTSALLARLRSEQPEGA"
     gene            complement(294..1271)
                     /locus_tag="VFQ61_22725"
     CDS             complement(294..1271)
                     /locus_tag="VFQ61_22725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HET9957338.1"
                     /translation="MAAKKAKPKGAPKTTRRRKGVKLRPTQLTAVQLAEVDRQGKLAQ
                     LAERVLADEGSVLAQYREPLGGHELLLVALPLERVEPTPFQRDVSDAHVRRLTRAMDK
                     TKRFLDPIIVTAVDGKYWTPNGNHRLTALRELGAKSIVAILVPEPEVAYHILALNIEK
                     AHALRERALEVVRMYRELGKLSRQTESVYELEFEEPALVTLGFAYEQRPRLAGGAYHA
                     VLRKVDSWIAAPLPSAAEERERRAALVVELDEAVNEAVARLKAKGLTSPYLKNFVVSR
                     VNPLRFIKGDPPPLDVLLPGMAKRARGMKTDKIKNEDLARTAGPPPDED"
     gene            complement(1390..1836)
                     /locus_tag="VFQ61_22730"
     CDS             complement(1390..1836)
                     /locus_tag="VFQ61_22730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957339.1"
                     /translation="MPQTAGQGTVPMATGGSQGLAGRAGGGESSTAGGTAGGSVVATG
                     GSTGGAPAFPPATFTQVYNQVFLANCLSSACHDNSRRQPYFGTKQDTYEFFVQPNVIF
                     PGEDPAWSGIYQIMAWEIMPPDPRPNVPPEQLDLVAGWIMAGALND"
     gene            complement(2497..2865)
                     /locus_tag="VFQ61_22735"
     CDS             complement(2497..2865)
                     /locus_tag="VFQ61_22735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957340.1"
                     /translation="MIRNHSLVALAVILLCSVQALVACGSDSDSDNGGVAGKCLSDED
                     ICQFKEGASTAAEIRNKLGNAQQYIGNEVASYMCQQIAGQQIVHNDLVNFSFDSNGVL
                     EDVIVLRQGSAATPPPSCSR"
     gene            3619..5739
                     /locus_tag="VFQ61_22740"
     CDS             3619..5739
                     /locus_tag="VFQ61_22740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HET9957341.1"
                     /translation="MPSSASQLRLVSPQATRTHCPYCAFQCGMEVSASRSDGRTPTIQ
                     ADPDFPVNRGQMCVKGFTAATLLDHPERIRTPKLRTREGKWASTSWDAALDFIAERVE
                     RIRERHGAAAVGAFGSGALTNEKAYLLGKFVRLVLRSPNIDYNGRYCMASAAAGQNRA
                     FGVDRGLPFPVSDIAQTEALLLWGSNCAETMPPIMQWVFEQKNRGGKLIVVDPRRSET
                     ARLATLHLQLTPGTDLALANGLLHLAIEHRLIDQAYIAERTEGFEEVRRSVLTSHPAH
                     VERVTGVPIHQQLQTVEYLARCASSMALSGRGMEQQSKGTDTVLSLINLMLALGRVGK
                     PASGYGCLTGQGNGQGGREHGQKADQLPGYRLIEDPDHRAHVARVWGVDASELPGKGK
                     SAYELLDALGPSGGVHGLLVFGSNVAVASPHASNIVSKLRALELLVVCDAFENETSEA
                     AHVLLPTTQWAEEEGTMTNLEGRVILRRRVQEPPPEVKTDIEILGALAARLGRPEGFQ
                     FESSRDVFEEFRRATAGGKADYSGLTYERLDRASGIFWPCPAENHPGTPRLFEQRFAR
                     PSGRALFHVVHYRAAAELPDLEFPLYFTTGRYKEHYNSGAQTRRVPALAEAKPEPRLQ
                     MHPQLAIRLEVTEGQGVLVESRRGSVRFRVTLSRDIRPDTLFAPFHWGGKQAANVLTN
                     AALDPTSRMPEFKICAVRARPLTD"
BASE COUNT         1068 a         1910 c         1737 g         1105 t
ORIGIN      
        1 gcgccctcgg gctgctcact ccgcaagcga gccaacagcg cggatgtccc cgtgttccac
       61 agctcctgaa tttgctgctc gatcgctcgg ccaatgccgg gcagctcttg cagccgattc
      121 tgctcgacca acgaggctag ctcgtccccc aaggtgtcga ccacttccgc cgcgttggca
      181 taggccttca ctttgaactt cggctctccg gcaaactcga gcaggccggc gatctcggca
      241 agccctcgtg atatctgcgt gaagctctgc acgtcttcgg tcctcctccg tcttcagtcc
      301 tcgtccggag gtgggcccgc cgtgcgcgcg agatcttcgt tcttgatctt gtcggtcttc
      361 atgccgcgcg cgcgctttgc catgcccggt agcagcacgt ccaagggcgg cggatcaccc
      421 ttgatgaagc gcagcgggtt cacgcgagag actacgaagt tcttcaggta cgggctggtc
      481 aagcccttgg ccttgaggcg cgccaccgct tcgttcacgg cttcatcgag ctccacgacc
      541 aacgccgccc ggcgctcccg ctcctcggcc gcacttggga gcggcgccgc gatccagcta
      601 tccactttgc ggagcactgc atgataagca ccaccggcca ggcgcgggcg ctgttcgtag
      661 gcgaagccca gagtgacgag tgccggttct tcgaactcga gctcgtagac cgattccgtc
      721 tgccgagaaa gctttcccaa ttcccgatac atgcgcacca cctcgagcgc gcgctcgcgc
      781 agcgcgtgcg ccttctcgat attcagcgcg aggatgtgat aggccacctc gggctcggga
      841 accaggattg cgacgatcga tttggcgccg agttcacgca gggcggtgag tcgatggttg
      901 ccgttgggag tccaatattt accgtcgacc gccgtgacga tgatcgggtc caaaaagcgc
      961 ttggttttgt ccatggcgcg ggtcaggcgg cgcacgtgtg cgtcgctgac atctcgctgg
     1021 aagggagtgg gctcgacccg ctcgagaggc aatgcgacga gtaggagctc gtgcccgccg
     1081 agcggttctc ggtactgcgc gagcacactc ccctcgtccg cgagcacgcg ctcggcgagc
     1141 tgcgccagtt ttccctgccg gtcgacttcc gctaattgca ccgctgtcag ctgcgtcgga
     1201 cgaagcttca cgcctttgcg acggcgtgtt gttttaggtg cacccttggg cttggctttc
     1261 ttcgcggcca cactcctgtt ctatcgacaa aacgcgctca cgccagccgc cgcatgctgc
     1321 acgagtgggc caaaacgccg cgcttttagt cgagcgtagt cgcgcattgc cccgcaatct
     1381 cccgatcgat caatcgttca acgcgcccgc catgatccaa cctgccacca ggtccagctg
     1441 ctccgggggc acgttcgggc gtgggtcggg cggcatgatt tcccaggcca tgatttggta
     1501 gatgccggac cacgccgggt cctcgccggg gaagatcacg ttcggttgca cgaagaactc
     1561 gtaggtgtcc tgcttggtgc caaaataggg ctgacgacgg ctattgtcgt ggcacgcgct
     1621 actcaggcag ttggctagaa agacctgatt gtagacttgg gtgaacgtcg ccggcgggaa
     1681 cgccggggcc ccacctgtgc tgccccccgt cgcaacgacg ctcccgccag cagtcccacc
     1741 cgcggtgctc gattccccgc caccggctct tccggctaag ccttgcgagc cgccggtcgc
     1801 catcgggacg gtcccttgcc cggccgtctg cggcaagggg gctccggcaa atccaccgtt
     1861 tccctgacca gtcgaattcg tgccgccggg ggttcctgcg ccggctccgt taccgagtcc
     1921 gccaaaaccg gcgctggagg ttccaccgac tgccgaaggc gcgccccctt tcgatatttc
     1981 cgatccacca aacccgccgg aagttgttcc cccacgcgca tcggtgccgc ttggggccga
     2041 gggcgaactc ccgctctgtg ttgattgcga gccaccactc tgtgttgatt ccgagccacc
     2101 actctgtgtt gattccgagc caacgctctg tgttgattgc gagccaccgg tggtgatttg
     2161 catgggagtg ccattcgcga gagtgccgcc agctccggct gcaggttgcg caggagggct
     2221 tgcagagcat gcgccaatca cgctcgcacc gagcaccaaa cgcgcgcaac gcagcagact
     2281 cgcgtgagat cgcattgcgc gcagactacc aactccgacg cagccaagcg agcccgctgc
     2341 cccacggtgt gaacgatgac tccgtcgagg ccacaagact taagtcccct tgaattgcat
     2401 ggctctccgc ttggccgccg acgacggcgt tggcggctcg tcgctgcaga gggtcaaatt
     2461 cttgttgggc gcggctgcga ttttgctggc gcctattcag cgcgagcagc tcggcggcgg
     2521 cgtcgccgcg ctgccttgtc gcagaacgat gacgtcttcg agcacaccgt tcgaatcgaa
     2581 gctgaagttc accaaatcgt tatgaacgat ctgttgaccc gcgatttgct gacacatgta
     2641 gcttgcaacc tcgttgccga tatactgctg cgcgttcccg agcttattcc taatctcggc
     2701 ggcggtactc gcgccctctt tgaactgaca aatgtcctca tcggatagac actttccggc
     2761 gacacctccg ttgtccgaat cggaatcgga accgcacgcg accagagcct gcaccgaaca
     2821 gagtagaata acggccaaag caaccaagct atgatttcga atcattatat atctcacgat
     2881 cagaagcacc gctcgcacgg cgttccgatg ataccctagg acaccagtcg agatcgacat
     2941 ggacctcatc ggttttcggc gaagcgcgtg tgttcgtttg gacgcaaccc tgcttgctcc
     3001 ggtccatttg gctacccgag cagaaagcag ccgagtacga ccggtgcccg agcagtccgg
     3061 taatctcgcg cgtcgcctga ctctcgtggt cccccactcc agcggcgccg gcccgcagaa
     3121 cctggtcgag aacggcggct ctgccgagca cgtggccaag cacgatcggg aacgcgcgcc
     3181 gcctctcggc gatgaagccc tactcgtgtc gacgatccca cggatcacga ctcgcccatg
     3241 tggaaactca agcgacggaa aagctcgctc atcgagctgg ggctgctccc agccggccat
     3301 actacctggc cgtcgctcgg tacatctcga ttcccgcggc catgacctcg gagagttccc
     3361 ctctcttcga gcaagcccta cgagttcgag atcgattgcg cccaattcga ggcggctttg
     3421 attcaccctg tcgggtagga ttgacagagg gtctgaatgg agccgatcgc gggaatcagc
     3481 gctcctcgta ggcccggaag acgaggacca gagtccacgt atcgaggatc gatagaatcg
     3541 atcctcgata cttttaaccg tcggcagacc ttccggacat cgccttgaaa ccgggcgtct
     3601 cctacgttcg atccggtcat gccgagcagc gcctctcaac ttcggctcgt gtcgccccaa
     3661 gcgacccgaa cccattgccc ttactgcgcg tttcagtgtg ggatggaggt cagcgcttcg
     3721 cgctcggatg ggcgcactcc aaccatccaa gccgaccccg attttccggt caatcgggga
     3781 caaatgtgcg tcaagggctt cacggccgcg accctgctcg atcaccccga acgtatacgc
     3841 actcccaagc tgcgcacgcg cgaggggaag tgggcatcta ccagttggga cgcggccctc
     3901 gacttcatcg ccgagcgggt cgagcgcatc cgcgagcggc atggcgcggc cgcggtcgga
     3961 gctttcggca gtggcgccct gaccaacgag aaggcgtacc tactcggcaa gttcgtgcgg
     4021 ctcgtgctgc gctcccccaa tatcgattac aacggccgct actgtatggc gtcggccgcc
     4081 gcgggccaaa atcgcgcgtt cggtgttgat cgcggcctgc ccttccccgt ctcggacatt
     4141 gcgcagacgg aagcgctcct gctgtggggt tccaattgcg cggagaccat gccacccatc
     4201 atgcagtggg tgttcgagca gaagaaccgc ggcggcaagc tgattgtcgt ggatccccgc
     4261 cgcagcgaga cagctcggct ggcaacgctg catcttcagc tcacccccgg cacggacctg
     4321 gccctcgcca acggccttct ccacctcgcc attgaacatc ggctgatcga ccaggcgtac
     4381 atcgccgagc gcaccgaggg cttcgaggag gttcgacgga gcgtgttgac gagccatcca
     4441 gcgcacgtgg agagggtcac gggtgtcccc atccaccaac agctccagac cgtcgaatac
     4501 ctggctcgct gcgcctccag catggcgctc tcgggccgtg ggatggagca gcagtccaaa
     4561 ggaaccgaca ccgtactgtc gctcatcaat ctgatgctgg ccctcggccg agtcggcaaa
     4621 cccgccagtg gatatggttg tctaacggga caaggtaacg ggcaaggcgg ccgcgaacac
     4681 ggccaaaaag cagaccaatt gcccggatat cgcctgatcg aagatcctga ccatcgtgcg
     4741 cacgtggccc gcgtctgggg tgtagatgcg tcagagctgc ccggcaaagg caagagtgcc
     4801 tacgagttgc tcgacgcgct cggaccaagc ggtggagtac acggcctgtt ggtcttcggc
     4861 tcgaacgtcg ctgtggcgtc gcctcacgct tcgaacatcg tcagcaagct gcgtgcgctc
     4921 gagctcttgg tggtgtgcga cgccttcgag aacgaaacgt cggaggcggc gcacgttctg
     4981 ctcccaacca cgcagtgggc cgaagaggaa ggcaccatga ccaacctgga gggccgcgtc
     5041 attttgaggc gccgggtgca ggaacctccc ccggaggtga agacggacat cgaaatcctc
     5101 ggcgcactag cagcgcggct cggtcgtcca gagggttttc aattcgaatc ttcgcgcgac
     5161 gtattcgaag agtttcgccg cgccaccgcg ggcgggaaag ccgactacag cggactgacc
     5221 tacgagcgct tagaccgagc atcaggcatc ttttggccgt gcccggccga gaatcacccc
     5281 ggaactccgc gcctgttcga gcagcgcttt gcccgcccct cggggcgggc gttgttccac
     5341 gtcgtgcatt atcgcgccgc cgccgagctt cccgatctgg agtttcctct gtacttcacg
     5401 accggtagat acaaggaaca ctacaactcc ggtgcgcaaa cgcggcgcgt cccggcactg
     5461 gccgaggcca aaccagaacc gaggctgcaa atgcacccgc agctcgcgat tcgcctcgag
     5521 gtaacggaag gccagggcgt gttggtggaa agccgacgag gcagcgtacg tttccgcgtc
     5581 actctttcga gagatattcg accagatacc ttatttgctc ccttccattg gggaggcaaa
     5641 caagccgcga acgtactcac caacgcggcg ctggacccaa ccagccgcat gcccgagttc
     5701 aagatttgcg cagtgcgtgc gcgccccctc accgactgag cggcgagacc tcaagtaaat
     5761 ggcgaaaaag aagctggcta tcattggtaa cggaatgggc acctgccggt tgctggacga
//
LOCUS       DASTKU010000652         3064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_570718, whole genome shotgun sequence.
ACCESSION   DASTKU010000652 DASTKU010000000
VERSION     DASTKU010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570718"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VFQ61_22745"
     CDS             <1..370
                     /locus_tag="VFQ61_22745"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9957342.1"
                     /translation="ALERGRLICIDELEASLHPMLSLAILNLFQDPQRNPRQAQLIFT
                     THDTNLLGTVLGEPPLRRDQIWFTEKDTSGATHLYPLTDFHPRKQENLERGYLQGRYG
                     AIPFLGNLLAYETSTGEGSE"
     gene            378..1004
                     /locus_tag="VFQ61_22750"
     CDS             378..1004
                     /locus_tag="VFQ61_22750"
                     /inference="COORDINATES: protein motif:HMM:NF025084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HET9957343.1"
                     /translation="MVSKRRLDNRDYKRRGPKRDRKFLILVVCEGAVTEREYLKSLQH
                     HFRNPRVRVVVPDNSGVPLTVVKTAIRLKKEAAQKAKAQDDDNLEYNSIWAVFDVDEH
                     PNLAEAKQLAAKEGIDLAISNPCFELWALLHLEDQRAHIERHAVASRLRKHFPEYEKL
                     LPFERLIGGYEQAVNRARSLDEEAASHKEPGRNPTTGVFSLTEQIRTR"
     gene            complement(1061..1564)
                     /locus_tag="VFQ61_22755"
     CDS             complement(1061..1564)
                     /locus_tag="VFQ61_22755"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET9957344.1"
                     /translation="MDQSPKKTPERSRVTLHQLMLPEHGNALGNVHGGLIMKMVDEAG
                     AIAAMRHAQRPCVTIAIDSMTFRQPVHLGELLVCDARVTYTGRSSIEVIVHVHSENPI
                     TAQVTHTNSAHLVYVALGEDGRPCEVPPIERVTEEDERAYAEGELRQRERLARKQREA
                     AVPPPPR"
     gene            1664..>3064
                     /gene="rseP"
                     /locus_tag="VFQ61_22760"
     CDS             1664..>3064
                     /gene="rseP"
                     /locus_tag="VFQ61_22760"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET9957345.1"
                     /translation="MDVVYFIVLVSSLIFVHELGHFLFAKAFGVKVLTFSLGFGPKIL
                     KLRGRETEYCISLLPLGGYVRMLEESKSDLVLPEDRRRTFESLPFIKRVLVVLAGPLM
                     NLIFPVLLYFSVFVGEGPLLPPTVGVVLPNHPADGKLQPGDRIMSVNGQEIGTFDELK
                     RKIAKSPGKNLRFKVFRENKHIDVEVTAEDTVERHQLDIVERIGTIGIQPSPPAAVIG
                     VPNSESPAYRAGLRTFDWITHVGGAPVRRFADLTEALGENAGETLPVTYLRPVFLPDA
                     LGGMADMAVFEAGVVALTPDASGSTLLERTGIELADLYAAIVPEDSVFYKAELRPGDR
                     ILRLDEEPVPAWSTFYERLLAEPDRPHRIDFESSRDGRARTGTIQLRREEFTDEHGQS
                     FEKYVLPLQHWVPVAPEQRVSHPTPIRYALEKAVEETVDVTRFVLVGIVRVIQGRVSL
                     KSLSGPITIFQVAGEEG"
BASE COUNT          555 a          938 c          975 g          596 t
ORIGIN      
        1 agcgctcgaa cgtggccgac tgatctgcat cgacgaactg gaggcgagcc ttcacccgat
       61 gctgtcgcta gctattttga acctgtttca agacccgcag cggaaccccc gccaggcgca
      121 gctgatcttc accacgcacg atacgaattt gctgggaacg gtcttggggg agcctcccct
      181 gcggcgcgac caaatctggt tcacggaaaa agacacgagc ggggccactc atctgtaccc
      241 gctcaccgat tttcatccgc gtaagcaaga gaacctggaa cgcggctatc tgcagggacg
      301 ctacggcgcg atcccattcc tcggaaacct gctcgcctac gagacgtcga ctggcgaggg
      361 gtcggaatag cggtccgatg gtctcgaagc gccgactcga taatcgcgac tacaaacgga
      421 ggggccccaa gcgggaccgt aagttcctca tcctggtggt gtgcgaagga gccgtcaccg
      481 agcgagaata cctcaagtct ctccagcacc atttccgcaa cccccgcgtt cgcgttgtag
      541 tccccgacaa ctcgggcgta ccgctcaccg tcgtcaaaac ggccattcgc ctcaaaaagg
      601 aagctgccca aaaggccaaa gcgcaggacg acgacaatct ggaatacaac tctatttggg
      661 ccgtgttcga tgtggacgag cacccgaatt tggctgaagc caaacagctg gctgccaagg
      721 agggcatcga tcttgctatc tcgaaccctt gcttcgaact ctgggcgctg ctgcacctcg
      781 aggatcagag agcccacatt gagcgccacg cagtcgcttc acggctgagg aaacacttcc
      841 ccgagtacga aaaactcctg ccattcgagc gtttgatcgg aggttacgaa caggctgtca
      901 atcgtgcccg gtcgctcgac gaagaagcgg cgagccacaa ggaaccgggg cgaaacccga
      961 ccaccggcgt gttcagtctc actgagcaga tccgaacccg ttaaatgact gagcacggcc
     1021 cggcgaccgt agaactcgct tccgaaggcg ctccctatgt tcaacgcggt ggaggcggca
     1081 ccgccgcttc gcgttgtttg cgggcgaggc gctcgcgctg gcgcagttcg ccttcggcgt
     1141 aggcgcgctc gtcttcttcc gtaacccgct cgatcggagg cacctcgcag gggcggccgt
     1201 cttctccgag ggcgacgtag actaagtgcg cggagttggt gtgtgtcact tgagcggtga
     1261 tcgggttctc ggaatggacg tgcacgatga cctcgatcga gctgcgaccc gtgtaggtga
     1321 cgcgcgcgtc gcagaccaag agctcaccga gatgaacggg ttggcggaag gtcatcgagt
     1381 cgatcgcgat cgtgacgcag ggccgctggg cgtggcgcat ggccgcaatg gctccggctt
     1441 cgtccaccat cttcatgatc aggccaccgt gcacatttcc gagtgcgttg ccgtgttcgg
     1501 gcaacatcag ttggtgcagc gttacccgtg agcgttccgg ggtcttcttc gggctttggt
     1561 ccatcgccgg gctaggctac ccgctggccg aagcacgtgc tagcgtcgcg cgtcttccgg
     1621 cgctcgagcc gcttcatgct tcgccttctt cccttctgaa acgatggacg tcgtctactt
     1681 catcgtgctc gtcagctcgc tgatcttcgt gcacgagctc ggtcacttcc tgtttgcgaa
     1741 ggcgttcggc gtcaaagtgc tcacgttcag cttgggcttc ggtccgaaga tcctgaagct
     1801 gcgcgggcgg gagaccgagt actgcatttc gctgctgccc ctcgggggtt acgtgcggat
     1861 gctcgaggag tccaagagcg acttggtgtt gcccgaggat cgacgtcgca cgttcgagtc
     1921 gctgccgttc atcaagcgcg tgctggtggt gctcgccggc ccgttgatga atctcatttt
     1981 cccggtcctg ctctacttct cggtgttcgt tggggagggg ccgttgttgc cgccgacggt
     2041 cggggtggtg ctgccgaacc acccggcgga cggcaagctg cagccggggg atcgtatcat
     2101 gagcgtcaac ggccaagaga tcggcacgtt cgacgagctg aagcgcaaga tcgcgaagag
     2161 ccccggcaag aacttgcgct tcaaagtctt ccgcgagaac aagcacatcg acgtcgaggt
     2221 gacggcggaa gataccgtcg agcggcacca gctcgacatc gtcgagcgca tcggaacgat
     2281 cggcattcaa ccgagcccgc ccgcggccgt gatcggcgta cccaactcgg agtccccagc
     2341 ttaccgcgcc ggcctgcgca ccttcgattg gatcacccat gtgggtggtg ctccagtgcg
     2401 ccgctttgcc gatttgaccg aggcgctggg cgagaatgcg ggtgagactt tgcccgtgac
     2461 ctacttgcga ccggtgtttc tgccggacgc gctcggcggg atggccgaca tggcggtgtt
     2521 cgaggcgggc gtggtggcgc tcacgccgga cgcgagtggc agcaccctgc tggagcgtac
     2581 tggcatcgaa ctcgcggatc tctacgcggc gatcgtgccc gaggactcgg tgttctacaa
     2641 agccgagctc cgtccggggg atcgcatctt gcgtttggat gaagagcccg tgccggcgtg
     2701 gtccaccttc tacgagcggc tgctcgcgga acccgatcgc ccccaccgca tcgatttcga
     2761 gtcttctcgc gacgggcggg cgcgcacggg gaccatccag ctgcgtcgtg aggagttcac
     2821 ggacgagcac ggccagtctt tcgaaaagta cgtgctgcca ctccagcatt gggtgccggt
     2881 ggcgcccgag cagcgcgtct cgcatccgac ccccattcgc tatgccttgg agaaggcggt
     2941 cgaagagacg gtggatgtga cccgcttcgt gctcgtgggt atcgtgaggg tgatccaggg
     3001 gcgggtcagc ctcaagtctt tgtcgggccc aatcaccatc tttcaagtgg cgggggagga
     3061 gggg
//
LOCUS       DASTKU010000653        14185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1464886, whole genome shotgun sequence.
ACCESSION   DASTKU010000653 DASTKU010000000
VERSION     DASTKU010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14185
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464886"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VFQ61_22765"
     CDS             <1..376
                     /locus_tag="VFQ61_22765"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9957346.1"
                     /translation="YGVLDADHHQLRAASAGAFPFPLLRRGANGAGSELECSGRPLNL
                     PGPRQFGTVETSLPSGSALLLASDGVLELEPKGRPRDKRRELANLLERAHSLQDLLRG
                     LSLDEATRLEDDVALLFIQREA"
     gene            380..865
                     /locus_tag="VFQ61_22770"
     CDS             380..865
                     /locus_tag="VFQ61_22770"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET9957347.1"
                     /translation="MAKGHVTHAEHDGVHVLRYFGRVDYMAACGIQKFLDSLVEQGEL
                     SGLVFDLTDAEALDSTNLGLLARMRERVRARRLAESLIVSRNEDINDVLYSMGFDRIF
                     QIVTDAPAASKDGSDPIDGAATSQEELRKTMLDAHRALLELSDRGRAEFQDVVACLEK
                     Q"
     gene            complement(965..1972)
                     /locus_tag="VFQ61_22775"
     CDS             complement(965..1972)
                     /locus_tag="VFQ61_22775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957348.1"
                     /translation="MDGSADVEPVPLDAPIVVRLSRPLLPASAVRQAVSVYSGQPGMA
                     SPFLAPHYDVLNRVLVYWTIGQFTPEALYQIQFAIPAAAQGFGLRAFDGTPLSNTSPF
                     LRTSFVTSDRTAALPVLPKPEDTAEQEMPGQPQTEPPKINPQQAEPTCREVVELFKAS
                     CASSCCHGGAAPAMALALDSEEGLLRTAIGKVAHQARAPEVEGVPFVDPDRFGNAMAI
                     IQPGSPATSYLVYKMLLLPENLESPSPVTGSQLDASAKTSSHVLGLSLPDEACDPLDE
                     QERTRLSNWFVHGEAMPPSWVARSRPGTADCSLPSRAALGCSEFRRINRFIELGAHCS
                     P"
     gene            complement(2167..2421)
                     /locus_tag="VFQ61_22780"
     CDS             complement(2167..2421)
                     /locus_tag="VFQ61_22780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957349.1"
                     /translation="MKELVRFLFENLYFDFQGDITMEKVREFLREDDSREARALLSKI
                     IEERGVDDMLITLADCLKEHIRTGVSEQIIRDQLSTYSDS"
     gene            complement(2652..3956)
                     /locus_tag="VFQ61_22785"
     CDS             complement(2652..3956)
                     /locus_tag="VFQ61_22785"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HET9957350.1"
                     /translation="MNDRSSLTWLVWVACGLAALLGISAAPAAPAPLEARTAASASVS
                     AQPAPELRFAVRSAVIYPSTSEVLRFNHERHVTELRLPCTACHDAARRSRRAEDRLRP
                     PPARCDGCHGSDHSDLSHVKGDESEPSSRCQVCHLGYQPEHGNAVARFVNKPAHLKFN
                     HAVHVAAKLGCEGCHAGVEHSATAGREHLPSMQQCVTCHRLNAGGPRHPSGECAVCHV
                     TAGRRLQTHFAEGRLLPPAWLGHAEHDPAFAMRHAVLAANDSGLCAKCHVEKDCADCH
                     DGRVRPRRLHPNDFLSMHAVAARHDRPRCASCHQQQTFCLSCHQRLGVSSSSPTALAA
                     ERGRFHPPSQIFVEGPRSARHHAVEAQRNLNVCVACHTERDCVSCHATREIGAGGLSA
                     DGARLNPHPPGFSARCARLLSQNPRPCLVCHELSDARLQECR"
     gene            complement(3953..5431)
                     /locus_tag="VFQ61_22790"
     CDS             complement(3953..5431)
                     /locus_tag="VFQ61_22790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957351.1"
                     /translation="MLIIRPRAAGAYDARIDAALDAQYYMLRSPFGEPRFYRRRYTST
                     LGLDVENLAGPSSLRGPRLDFRSRVRLDTDFGRDAAATDPQSNRYLPGLEQGPLDVMY
                     AYLEGSGYWHGFLGFRVGRQYVMDALGYWGFDGGLLSLRLPGVQLSGYVGTEQRSGLP
                     VLSTSRFGGSGVWRGDRTGLEDNQYPAFLSESALAPAVGVAIETRDVGWLHARLGYRR
                     VENRSRVLVSPFLEPGRPLVFVDQARVSSERLGGSLTLDAGSSFTLSARGAYDLFQRT
                     LADASATLDATLGSNLNAGLEYSYYYPTFDADSIFNYFRRGASSTGLVRVDTRRGAWD
                     LAVSSGARWFMIPDGGAVFPFFEGSVSNAESGTAARAASLFLADAGARCAIPSGSITM
                     DASVELGAESHRVGADVATRRAFAGGRYDSLVILSVHDFRDALREERSATSFSYVLGG
                     GVRPGAMLISRGRLGFEWEHSVNRLVGHRMRLLLTMDFTVLQ"
     gene            5698..6960
                     /locus_tag="VFQ61_22795"
     CDS             5698..6960
                     /locus_tag="VFQ61_22795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957352.1"
                     /translation="MRRGYSIAITLLTLLACSSEKEPTTSTGQGGGGAGATQSGGSAG
                     MGGNHSAGSSTASGASAGGSSSGGAHSGGGSQPAGAGASNSAGSTSGPSGSGSPPATG
                     ICGAAPGQWFGSDYPFNQRIDQAALDTESRAIVEYLQANHRDSRRFRIDGPSDEANNV
                     YGLVLLGTDAGTIRQSFEATDDFYDPDCDPAPIPLPARGAIEGETSYACEEDGDCHLI
                     VVDAEQCKLHEMWRANRSSPSSFSGGCQAIWDLRAAYTPTLRGDCCTSADAAGLPILA
                     QTFTADEIAAGRIAHAIRFILPNEHIRERIYVRPATHATDTTGPANAPPYGVRMRLKA
                     GFDDSGLKPAARVVVRALKEYGMILSDGGNLTFTAANDRFTTHKWSEVDLMPGDLTSL
                     NWSDFEVPELGERYTWDDNCECRRTPIR"
     gene            complement(7011..9317)
                     /gene="pilQ"
                     /locus_tag="VFQ61_22800"
     CDS             complement(7011..9317)
                     /gene="pilQ"
                     /locus_tag="VFQ61_22800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HET9957353.1"
                     /translation="MRLAVPIQIESKRLASSALALLLALSLAHPAAAAEPLRHATKLT
                     VAPESAGETRIRIQFSAAPTFTARLEQQGVRLIVDVPNADLVNVPAALTERVGMVTGV
                     LTQTFKPPGATGSSTLRVLVMLSEPAKYAVIADGNDLVLRFASGELPPLSADRATVPA
                     ASAALPRVSDVKYEHTSDSDRIRIELSERVEYSVSEPDPSRARLTLRGSLPEELERRL
                     DVSAFHGKVSAVSSFAGEAQQVVFEVERAPGVKGRVERRGTALDWIFDAASPLPSTVS
                     GVGADGKAARRVKTVHVESPTAGIAEVHTGFEGQAIETRSNPDEAHAFGASVLGQQRK
                     AYTGRRIDLDLKDADVHNVLRLLADVGQVNIVTADNVSGTITIRMRNVPWDQALDIVL
                     QAKSLGMVRQANMIRVAPLDVLEKEREMQIARRQQEVKLAPIETRLVPISYANAEDLQ
                     ERARPLLSERGSLAVDERTNVLIARDLAGNLNQIEELVRSLDTQTPQVLVEARIVEAT
                     SRYLRDVGIQWGGDATFSGATGNPTGLAFPNSVGVVGGASDAQTPTAGLSPFANSVSN
                     PNFAVNLPAAVGTGQGGAIGLTFGSIDNNFNLALRLSAAEASGMLRILSSPRVLTLDN
                     QQARISQGTLIPFSQVSAQGVQTTFQEAKLQLLVRPHVTADGSISMHVKINRDEPDFN
                     QTSARGDPTILKREAETHLLVMDGHTAVIGGIYTRNTGRNLDQVPFFGDIPLLGLLFQ
                     RRRASDSRSELVIFLTPRIVNRAEALGR"
     gene            complement(9559..10170)
                     /locus_tag="VFQ61_22805"
     CDS             complement(9559..10170)
                     /locus_tag="VFQ61_22805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HET9957354.1"
                     /translation="MKRPAGIAFAVLGFCAACVSATACEDKVVRGVDVPTPPANAAAL
                     ASAAAAKPIPTVTIDESEFTESDRSRDPFRSYARTFVVQPTHGPVHQTNVVSPEYSID
                     ELKLVGIVTRIQPAKAMLVDPKGQGHVVQRGQFVGKADVVQTAGRAGSTYEINWRVDR
                     IREGDVVLVRVDPANPDVPSVTRVIPLRTEPTTDLGAAASVRN"
     gene            complement(10167..10787)
                     /gene="pilO"
                     /locus_tag="VFQ61_22810"
     CDS             complement(10167..10787)
                     /gene="pilO"
                     /locus_tag="VFQ61_22810"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HET9957355.1"
                     /translation="MARADLSLARLPTAAKVGISAAMVALVGAAYYIVFYDDLTTSMA
                     AAKGQEQQLHTQVSDAHKAEFAYQKDLAELTAKQQRQRELNKVLPVDTEYPAFLSAVQ
                     NVATVSGVGLTAWSPNPEVTEQFYARVPMRVELVGRFHQLAKFFYGVSQLDRIINMEN
                     ITLADPKAVGEDVVLRAEGSAVAFRAIEAGAPVPQDKRGAAQGAKP"
     gene            complement(10793..11431)
                     /locus_tag="VFQ61_22815"
     CDS             complement(10793..11431)
                     /locus_tag="VFQ61_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HET9957356.1"
                     /translation="MIRINLLSHKKRVDRQEKTQVWLLVAVGLVLVEVVILLVFHSLK
                     VEKLHDQERTNAELAARLDQLKKAVANHEEVKQKLAQLRAREDAITKLQTARTGPTLL
                     LMELARILTPGRGPTMDAAKLSQLRRDDPTAAYSSTWDPQRLWLTTFKEEKRKLRIEG
                     LARDGEDVSELARRMHLSDYFSNVRLLPAKREIEPVSKTEVVRFALEAEAKY"
     gene            complement(11428..12501)
                     /gene="pilM"
                     /locus_tag="VFQ61_22820"
     CDS             complement(11428..12501)
                     /gene="pilM"
                     /locus_tag="VFQ61_22820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HET9957357.1"
                     /translation="MGEGKNLVGVDIGSASIKVCQVRETRRGLGLIRMGYVPLGPQVI
                     VDGQVMDSGAVVEALGKAFTDAKIRQKECAVSVSGQSVIIRKITVPMMTPAELNEQIQ
                     WEAEQHIPFDIKDVQVDYQVLRKRPELSQMDLLLVAAKKDRIDDYAQLARSAKLRPVV
                     CDIDSFTVQNLFEFSRGLPEDKTIALINVGASFSSLSIIANGVHVFPREIANGGNAIT
                     EEIQKQLGLPFEQAEVYKCGGSSNDPTRGGMVPQQVVQVVEAVTDSIAAEIQRSIDFY
                     LATSGEGEISRIYVTGGTANLPSLARAIERRARVPVETWLPTEKVTVEAKEVNGQLLM
                     QHAAQLSVALGLSLRKEREVRAA"
     gene            13110..13691
                     /locus_tag="VFQ61_22825"
     CDS             13110..13691
                     /locus_tag="VFQ61_22825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9957358.1"
                     /translation="MLLEEYTPLVRKIAGGFQRKLPRNVLREDLLAAGMSGLWDAIRR
                     HGDTAGETFEWYVRVRVRGAILDELRAQDWLPRRARAAAATASAESGEYVPPPAVVRL
                     DDVSEWEQSRCLSDMSSSETAVAAKFTHETLAKAVELLPERERHIVSQHYFRGVKFKD
                     LGAELGVSEPRISQLHSRAMRRLKTLMAEAQQI"
     gene            13923..>14185
                     /locus_tag="VFQ61_22830"
     CDS             13923..>14185
                     /locus_tag="VFQ61_22830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957359.1"
                     /translation="MKPTLSALLITAAFAGCSNSNTGSVPPGAPPSNSTGGATAGSSP
                     VATGGNSVASTSGGTISAAGGATSATGGAVSASGGASAAGGSST"
BASE COUNT         2630 a         4693 c         4431 g         2431 t
ORIGIN      
        1 ctacggtgtc ttggacgcgg accaccacca gctgcgcgcc gcgagcgcag gagcgttccc
       61 tttccccctg ttgcgacgcg gggccaacgg cgcgggcagc gaactggagt gcagcggccg
      121 tcccttgaac ttgcctgggc cacgccaatt cgggacggtg gagacgagcc tgccgagcgg
      181 cagcgcgcta ctcttggctt cggacggcgt actcgaactc gaacccaagg ggcgaccgcg
      241 ggacaagcgg cgggagctcg ccaatttgtt ggaacgagcc cacagcttgc aagacttgct
      301 gcgcggtttg tccctggacg aggccactcg gctcgaagac gacgtggcct tacttttcat
      361 ccaacgggag gcatagagca tggccaaggg tcacgtcact cacgcggaac acgacggcgt
      421 tcatgtgctt cgttatttcg gccgcgtcga ctacatggcc gcctgcggga tccagaaatt
      481 cttggactca ctggtggagc aaggcgagct ctctggcttg gtcttcgatc tgacggatgc
      541 cgaggcgctg gacagcacga acctgggcct gctcgcgcgc atgcgggagc gcgttcgcgc
      601 ccgaaggctg gccgagagct taatcgtctc gcgaaatgaa gatatcaacg acgtgctcta
      661 cagcatggga ttcgatcgca tcttccaaat cgtgacggat gctccggcgg catcgaagga
      721 tggttcggac ccgatcgacg gggctgccac ttctcaggag gagcttcgca agacgatgct
      781 cgatgcgcat cgcgcgttgc tggagctaag tgaccgcggg cgtgcagagt ttcaagacgt
      841 ggtcgcctgc ctcgaaaaac agtgataacc gcgctcgcgg accgcttcgc gtcgcgctcc
      901 gcgctcgacg acgcacaaac ttcttgggcg cggctcaggg cgagcaggct cagggtgagc
      961 aggctcaggg cgagcaatgg gcgccaagct cgatgaagcg attgatgcga cgaaactccg
     1021 aacagccgag tgcggctcgg ctgggcagcg aacaatcggc cgtgccgggc cgtgatcgcg
     1081 caacccagct cggcggcata gcctcaccgt gcacgaacca gttcgatagt cgcgtgcgct
     1141 cttgctcgtc cagcggatcg cacgcctcgt cgggcaggct caagccgagc acatgcgagg
     1201 acgtcttggc ggacgcatcg agctgagagc ctgttaccgg cgatgggctc tcgagattct
     1261 ccggcagcag caacattttg tagacgagat agctggtagc ggggctgccc ggctgaatga
     1321 tagccatcgc attgccgaag cgatcgggat ccacgaacgg cacgccttcc acttcgggcg
     1381 cgcgcgcttg gtgcgcgacc ttgccgatcg cggttctcag caggccctcc tccgagtcca
     1441 acgccagcgc catcgccggc gcagctccgc cgtggcaaca gctgctcgca caagaagctt
     1501 tgaacagctc gaccacctcg cggcaggtgg gctctgcctg ctgcggattg atcttaggtg
     1561 gctcggtctg cggctgcccg ggcatctcct gctccgcagt atcctccggc ttcgggagca
     1621 cgggcaacgc ggccgttcga tccgaggtta cgaagctggt gcgcaagaag ggcgatgtat
     1681 tcgataacgg tgtgccgtcg aacgcgcgca agccaaagcc ttgagcggca gctgggatcg
     1741 caaattggat ttgatagagg gcctcgggtg tgaactggcc gatagtccag taaacgagca
     1801 cccgattcag aacgtcgtag tggggtgcca gaaatggcga tgccatcccc ggctggcccg
     1861 aataaaccga cacggcctga cgcaccgcgg acgccggcaa cagcggccgg ctaagccgca
     1921 ccacgatcgg tgcgtcgagc ggaaccggct ccacgtcagc agagccatcc atcggccgcg
     1981 gttcgtcagc tccaagctcg cccgcaagcc gcggcgaata accacgcggc gagatctcga
     2041 cgatacggag cggtggcccg gccgctactt cgggcgatgc cggacccaaa tcgcagctag
     2101 cgagagccgc tccagccgca cagccggcgg cggcccgaat cgctaacaag agccaacgaa
     2161 accaacctag ctgtcgctgt aggtggacag ctgatcgcga atgatctgct cgctgacgcc
     2221 agtccgaatg tgctctttca aacagtcggc caacgtgatg agcatgtcgt cgacgccacg
     2281 ctcctcgata atcttcgaca gaagcgcacg ggcttcgcgg ctgtcgtcct cgcgcaggaa
     2341 ttcccgcact ttctccattg tgatatcgcc ctggaagtcg aagtacaggt tttcgaaaag
     2401 gaatcggacc agctccttca cctaatgcct ccttcgacgc cggccgggta agccccgaca
     2461 ctttgccgct atagccgccc gcagaacttt cctgcaagca gattgcgacc gatgggaaag
     2521 cggcgccgcc gcggttaata ccaccatccg ggcggctttg cctgaacctc gcctcgattg
     2581 ttctgggtcg ctcagaaacc gccaaaaaac ccggccccac gagacgcgca ctcgcgcagc
     2641 ctgcgtcggg ctcagcggca ctcctgcaaa cgcgcgtcgg acagctcatg acaaaccaaa
     2701 caagggcgcg ggttctggga cagaagacgc gcgcatcgag cagagaatcc cggaggatgg
     2761 gggtttagtc gcgcaccatc ggccgatagg ccgcctgctc cgatctctcg cgttgcgtgg
     2821 caagacacac agtcgcgctc tgtgtggcag gccacacaga cgttcaggtt tctctgagcc
     2881 tccaccgcgt gatgccgagc ggaccgcggg ccctccacga atatctgcga gggcggatga
     2941 aatcgtcctc tctccgcggc cagcgcggtc ggactgctgg aactcactcc cagccgttgg
     3001 tgacacgaga ggcagaacgt ctgctgctgg tggcacgagg cgcagcgcgg gcgatcgtgg
     3061 cgcgccgcga ccgcatgcat cgacaagaag tcgttcgggt gtaaacggcg cggccgcacc
     3121 cgcccatcgt gacagtcggc acagtccttc tccacgtggc acttcgcgca caagccgctg
     3181 tcgttcgcgg cgagcacggc gtgtcgcatc gcaaatgccg gatcgtgctc ggcgtgtcca
     3241 agccaggcgg gcggaagcag ccgcccctct gcgaagtgag tttgcagacg cctgccggcc
     3301 gtcacgtgac acacggcgca ttcaccactc ggatgacggg gcccgcccgc gttcaggcgg
     3361 tggcaagtca cgcactgttg catgctcggc aagtgctctc ggcctgccgt cgcgctgtgt
     3421 tcgacgcccg catgacaacc ttcgcagcca agcttcgccg cgacgtgcac ggcgtgattg
     3481 aattttagat gcgccggctt gttcacgaag cgagcgaccg cgttgccgtg ctccggctga
     3541 tatcccaggt ggcagacttg gcaccggctg ctcggttcgg attcgtcgcc cttcacgtgc
     3601 gagaggtccg agtgatcact gccgtgacag ccatcgcagc gtgcaggagg cgggcgcaac
     3661 cgatcctcgg cgcggcggct gcgccgggcg gcatcgtggc acgcggtgca cggcagacgc
     3721 agctcggtca cgtgccgctc gtggttgaag cgcaacactt cggacgtact cggatagatc
     3781 acggcgctcc gcaccgcgaa gcgcaactcc ggcgcgggct gtgcgctcac cgatgcgctc
     3841 gcggccgttc gcgcttcgag gggggcaggc gcggccggag ccgcagagat cccgagcagc
     3901 gcggcgagcc cacacgccac ccaaacgagc caagtgagcg agctcctgtc gttcattgga
     3961 gcaccgtaaa atccatggtc aggagcagac gcatgcggtg ccccaccagc cggttcacgc
     4021 tatgttccca ctcgaagccc agccggccgc gcgaaatcag catcgcgccg gggcgcacgc
     4081 cgccgcccaa cacgtaactg aaactggtgg cgctgcgctc ttcgcgcagt gcgtcgcgga
     4141 aatcgtggac tgacaagatc acgagcgaat cgtagcgacc accagcgaag gcgcgcctcg
     4201 tcgcaacgtc agccccgaca cgatgggatt cagcgcccaa ttcaacgctg gcatccatag
     4261 tgatcgagcc cgatggaatg gcgcagcgcg cgccggcgtc tgccaagaac agactggccg
     4321 cgcgcgccgc cgtccccgac tcggcgttgc tgacagagcc ctcgaaaaac ggaaagaccg
     4381 caccgccgtc ggggatcatg aaccagcgtg cgcccgagct gaccgccaaa tcccaagctc
     4441 cacgccgagt gtcgacgcgc actaggccag tcgatgaggc tccgcgccga aagtagttga
     4501 agatcgagtc tgcatcgaag gttggatagt aataggaata ttcgaggccc gcgttgaggt
     4561 tgcttccgag cgtcgcatcc aacgtcgcgc ttgcatccgc cagcgtgcgc tggaacagat
     4621 cgtaagcacc gcgcgctgac aacgtgaagg agcttcctgc atccaaagtc agagagccgc
     4681 ctaggcgttc actggaaact cgcgcttgat ccacgaacac caaaggacgc ccgggctcca
     4741 ggaaaggaga cacgagcact cgactgcggt tctctactcg tcgatagccc aagcgcgcgt
     4801 ggagccagcc cacgtctctt gtctcgatcg cgactcccac cgccggcgcg agcgcggact
     4861 cgctgagaaa cgccggatac tgattgtctt cgagcccagt gcggtccccg cgccacacgc
     4921 cactgccgcc gaagcgagac gtcgaaagta caggcaggcc gctccgctgc tcggtgccga
     4981 cgtagcccga gagctgaacc cctgggagcc gcagcgagag cagaccaccg tcgaaccccc
     5041 agtacccgag ggcgtccatc acgtactggc gaccgacgcg gaagcctagg aaaccgtgcc
     5101 aataaccgct cccttcgagg taagcgtaca tcacgtcgag cgggccctgc tccaaccccg
     5161 gcaagtagcg gttggattga gggtccgtcg cggccgcgtc acggccaaag tccgtatcga
     5221 gccgcacccg cgaacggaaa tcgagccggg gcccgcggag actactcgga ccggcgaggt
     5281 tctcgacatc gagccccagg gtcgaggtgt aacgccgacg ataaaagcgc ggctctccga
     5341 aggggcttcg cagcatgtag tactgcgcgt ccaacgcggc gtcgatgcgc gcatcgtacg
     5401 cgccggctgc gcgcggccgg atgatcaaca aggcgcacac gctgatcgcg ctgcaaaccg
     5461 cacacccagc ggcccgcagt agtcgcccgt tagatggcga ggagctccgc atcgacgggc
     5521 gcagaatcgt cgtgggctcg gcgcctgtca attgctgcgg cgctaggccc cgcgcgcctg
     5581 aaccccaaac tccatcccac tacaaactcc cccctaaaac acgcgcaggt cagaccgccg
     5641 tcgcgagccc cgtgccaaac taccggttcg tggatggttg cacggaaagg gacatgcatg
     5701 cgtcgaggct attcaatcgc gatcaccttg ctcaccctgt tggcctgctc atctgaaaaa
     5761 gagcctacga ccagcaccgg tcagggcggc ggcggtgcgg gcgctactca gtcgggaggg
     5821 agcgcaggaa tgggcgggaa tcacagcgca ggcagctcga ctgcctccgg cgcgagcgcg
     5881 ggcggctcga gcagcggtgg agcgcacagc ggtggtggga gccagccggc aggggcggga
     5941 gcaagcaatt ccgcggggtc gacgagtggc ccgagcggct cggggagccc acccgcaacc
     6001 gggatctgcg gcgccgcgcc agggcagtgg tttggctccg attacccgtt caatcagcgg
     6061 atcgatcagg ccgcgctgga cacagagtcg cgcgccatcg tcgagtatct ccaggccaat
     6121 caccgggatt cgcgccgctt ccgcatcgac ggtccgagtg acgaagccaa caacgtctac
     6181 ggcttggtgc tgctcgggac cgacgccggc accatccgtc aaagcttcga ggccacggat
     6241 gacttctacg atcccgattg cgatcccgcg ccaatcccgc tcccggcccg cggcgcgatc
     6301 gaaggtgaaa ctagctacgc ttgcgaggaa gatggcgact gccatctgat cgtggtcgat
     6361 gccgagcagt gcaagttgca cgagatgtgg cgcgccaatc gatcctcccc cagctctttt
     6421 tccggaggct gccaggcgat ttgggatttg cgtgccgcct atacaccaac cctgcgcggc
     6481 gactgctgca ccagcgcaga cgcagcgggc ttacccatct tggcgcagac cttcacggcc
     6541 gacgaaatcg cggccggccg tatcgcgcat gcgattcgct tcattctgcc gaacgagcac
     6601 attcgcgagc gcatctacgt gcgccccgcc actcacgcca ccgacacgac tggtcccgcc
     6661 aacgctcccc cctacggagt gcggatgcga ctcaaggccg gtttcgatga ttcagggttg
     6721 aaacccgcag cgcgcgtcgt ggtccgtgca ctgaaagagt acgggatgat cctctccgat
     6781 gggggcaatc tgacattcac cgcggccaac gatcgattca cgacccacaa atggagtgag
     6841 gtcgatctca tgccgggcga tctcaccagc ttgaattgga gtgacttcga ggtccctgag
     6901 ctcggggaac gctacacttg ggacgacaac tgcgagtgcc gccggacccc cattcgctga
     6961 acctacctgg accgtgctga ccaccgaggc agagcgcgct ccgctctgcc tcagcgcccg
     7021 agcgcctccg cccggttcac gatccgaggg gtcaggaaga tcacgagttc actgcggcta
     7081 tcgctcgcac gtcgacgttg gaacagcagc ccgagcaacg ggatatcccc aaagaaaggc
     7141 acctggtcca agttgcggcc cgtattgcgc gtgtaaatgc caccaatcac cgcggtgtga
     7201 ccgtccatca ccagcaggtg cgtctcggcc tcgcgcttga ggatcgtcgg gtcaccacgg
     7261 gccgaggtct gattgaaatc cggctcatcg cggttgatct tcacgtgcat gctgatgctg
     7321 ccatccgccg tcacgtgagg ccgcaccagg agctgcagct tcgcctcctg gaaggtcgtc
     7381 tgcacgcctt gggcgctgac ctgtgaaaac gggatcaacg taccttgcga gatgcgcgcc
     7441 tgctgattgt ccagggtgag cacgcgaggg ctgctgagga tgcgcagcat gccgctcgcc
     7501 tccgccgccg ataagcgcag cgcgaggttg aagttgttgt cgatcgagcc aaacgtgagc
     7561 ccgatcgcac caccttgccc ggtacccacc gccgccggca ggttgacggc gaagtttggg
     7621 ttggagacgc tgttggcaaa tgggctgagg cccgcggtcg gagtctgcgc gtcgctcgcg
     7681 ccgcccacca cgcccaccga gttcggaaac gccaatcccg tcggattacc ggtcgctccg
     7741 gagaacgtcg cgtcaccacc ccactgaata cccacgtcgc gcaaatagcg gctcgtggct
     7801 tcgacgatgc gcgcctcgac caggacctgg ggcgtctgcg tgtcgaggga gcgcacgagc
     7861 tcctcgattt gattcaagtt gcccgccaag tcgcgggcga tcagcacatt ggtccgctcg
     7921 tccaccgcga gcgatccgcg ctcggacagc aacggacggg cccgctcctg cagatcttct
     7981 gcgttcgcgt agctgatcgg aacgaggcgc gtctcgatgg gtgcgagctt cacctcctgt
     8041 tgccggcgcg cgatctgcat ctcgcgctcc ttctcgagca catcgagcgg cgccacgcgg
     8101 atcatgttgg cctggcgcac catgcccagg ctcttggcct gcagcacgat gtcgagcgcc
     8161 tgatcccagg gcacgttgcg cattcgaatc gtgatcgtgc cgctgacgtt gtcggcggtc
     8221 acgatattga cctggcctac gtccgcgagc agccgcagca cgttgtggac gtcggcgtcc
     8281 ttgaggtcca agtcgatgcg tcgaccggtg tacgccttcc gctgctggcc caacaccgaa
     8341 gccccgaagg catgcgcttc gtcggggttg ctgcgggtct cgatggcttg cccttcgaac
     8401 cccgtgtgca cctcggcgat gccggcggtg ggtgactcca cgtgcaccgt cttcacgcgc
     8461 cgcgccgctt tgccgtccgc gcccactccg ctaacggtcg agggcagcgg cgaagcggcg
     8521 tcgaagatcc aatccagcgc cgtgccccga cgttcgacgc ggcccttcac gcccggcgcg
     8581 cgctccacct cgaacacgac ctgctgggct tcgccggcaa agctcgaaac ggcgctcacc
     8641 ttaccgtgaa acgcggacac gtcgaggcgc cgctccagct cctccggcaa gctgccacgc
     8701 agcgtgagcc gcgcgcggct cgggtccggc tcggacacgg agtattcgac acgctcggag
     8761 agctcgattc ggatccgatc cgagtcggat gtgtgctcgt atttcacgtc ggatacgcgg
     8821 ggcagcgcgg ccgaggcggc cggaaccgtc gctcgatccg cgctcagagg tggaagttcg
     8881 ccgctcgcga agcgcaatac caaatcattg ccatccgcga tcaccgcgta cttcgcgggt
     8941 tcgctgagca tcaccagaac acgcaacgtg ctcgagcccg tggcgcctgg aggcttgaaa
     9001 gtttgcgtga gcacgcccgt gaccataccc acgcgctcgg ttaacgcggc cggcacattc
     9061 accaaatcag cgttgggcac atccacgatc aggcgcacgc cctgctgctc gagccgcgca
     9121 gtgaaggtcg gcgccgcgct gaactggatc cgaatccgcg tctcgcccgc gctctcgggt
     9181 gcgaccgtca acttcgtcgc gtgacgtagt ggttccgcag cggcggcagg atgggccaac
     9241 gaaagggcga ggagcaaggc caaggccgag cttgcgagcc gcttggactc gatctgaatc
     9301 ggaacagcta acctcatgac gaagctccag gatgctccct ctatcgcgag catcggacgc
     9361 ggtacgacag cctagtcgga ggaggattcc agaagcaaaa aaagggcacc cggccgtcac
     9421 gcgcacacct accccagacg accggccgcc gcgctggagc accgtttgcg gacgctgacg
     9481 ccgcccgcgg cgggtccaca accgagttct acgaactcct cgagagtggc ttctgcccgg
     9541 caattagcgc gaaaaaggct agtttcgcac gctcgcagcc gcccccaagt ccgtcgtcgg
     9601 ctcggtgcgg agggggatca ctcgggtcac ggaggggacg tcgggattcg cgggatccac
     9661 tcgcaccagc accacgtcac cttcacgaat ccgatcgacg cgccagttga tttcgtacgt
     9721 cgatccggca cgaccggcgg tctggacgac gtcggccttg ccgacgaact ggccgcgttg
     9781 caccacgtgc ccctgccctt tcgggtccac gagcatggcc ttggcaggct gaatccgcgt
     9841 cacgatgccc acgagcttga gctcatcgat cgagtactcg ggagagacca cattggtctg
     9901 gtgaacggga ccgtgcgtcg gctgaaccac gaacgtgcgg gcataagacc gaaacggatc
     9961 gcggctgcgg tcgctctcgg taaactcgct ctcatcgatg gtcacggtcg ggatcggctt
    10021 ggccgccgcg gcgctcgcga gagccgccgc attggcagga ggagtgggca cgtccactcc
    10081 tcgcaccacc ttgtcctcac acgcagtcgc gctgacacac gccgcacaaa acccgagcac
    10141 cgcgaaagca attccggcgg ggcgcttcat ggcttggctc cctgcgcggc cccgcgcttg
    10201 tcttgcggta ccggcgcgcc cgcttcgatg gctcggaacg ctacggccga gccttccgca
    10261 cgtagcacca cgtcttcgcc caccgccttg gggtcggcca gcgtgatgtt ctccatgttg
    10321 atgatgcgat cgagctgaga cacgccgtag aagaacttgg cgagttggtg gaagcgtccc
    10381 acgagctcga ctcgcatcgg cacccgcgca tagaactgct cggtgacctc cgggttcggg
    10441 gaccaggcgg tcaggcctac gcccgacacg gtagccacgt tttgcaccgc gctcaggaac
    10501 gccgggtact cggtgtccac tggcaatacc ttgttgagct cgcgttggcg ctgctgcttg
    10561 gccgtcagct ccgcgagatc cttttgataa gcgaactcgg ccttgtgagc gtcggagacc
    10621 tgcgtatgca gctgctgctc ttggcctttc gcagcggcca tactagtcgt caaatcatcg
    10681 taaaacacga tgtagtaagc cgccccgacc agcgcgacca tggccgcgga aataccgact
    10741 ttcgccgccg tgggcaggcg agcgaggctc aaatcagcgc gcgccatggc catcagtact
    10801 tcgcctccgc ttcgagcgcg aagcgcacga cctcggtctt cgacacgggc tctatctctc
    10861 gttttgcggg cagcagccgc acgttactga aatagtcgga gaggtgcatg cggcgcgcga
    10921 gttcgctcac gtcctcgcca tcgcgggcga ggccctcgat ccgcaacttg cgcttctcct
    10981 ctttgaacgt agtgagccac agccgctgcg ggtcccaggt ggaagagtaa gcggccgtag
    11041 gatcatcgcg ccgaagttga ctcagcttcg cggcatccat ggtgggccca cgaccgggcg
    11101 tcaaaatgcg agctaactcc atcaaaagca gcgtgggccc cgtgcgcgcg gtctgcagct
    11161 tggtgatggc gtcctcgcgg gccctgagtt gagccagctt ttgcttcacc tcttcgtggt
    11221 tggccacggc cttcttcagc tggtcgagcc gcgccgcgag ctccgcgttg gtacgttctt
    11281 ggtcgtgcag cttctccacc ttcaggctgt ggaagaccag caaaatgacg acctccacca
    11341 gcactagccc gacggctacg agcagccaaa cctgcgtctt ctcctggcga tcgacgcgct
    11401 tcttgtgcga gagcaggttg atgcgaatca tgccgctcgc acctctctct ccttgcgcaa
    11461 gctgaggccg agcgcgaccg agagctgagc cgcgtgctgc atcaacagtt gcccgttgac
    11521 ctctttggcc tcgacggtca ccttctccgt tggcagccag gtctccaccg gaacgcgcgc
    11581 acggcgttcg atggcgcggg ccagagacgg caggttcgcc gtgcccccgg tcacgtagat
    11641 cctcgagatc tctccctcgc cactggtggc caagtagaag tcgatgctgc gttgaatctc
    11701 ggcggcgatc gagtccgtaa ccgcctctac gacttgaacc acctgctgcg gaaccatgcc
    11761 gccgcgcgtc ggatcattgc tgcttccgcc gcacttatag acctccgcct gctcgaaggg
    11821 taacccgagt tgtttctgaa tttcctcggt gatggcgttg cccccattcg caatctcgcg
    11881 cgggaaaacg tgcacgccat tggcgatgat gctgagggac gagaagctcg ccccgacgtt
    11941 gatcagcgcg atcgtcttgt cttccgggag gccgcgggag aactcgaaca agttctggac
    12001 cgtgaaggag tcgatgtcac aaactacggg cctcagcttg gcgctgcgcg ccagctgcgc
    12061 gtaatcgtcg atgcgatcct tctttgccgc gacgagtaac agatccattt gactgagctc
    12121 gggccgcttg cggaggactt gatagtcgac ctgcacgtcc ttgatgtcga atgggatgtg
    12181 ctgctctgcc tcccactgga tctgctcgtt gagctcggct ggcgtcatca tcggcacggt
    12241 gatctttcgg atgatcacgc tctgtccgga gacgcttacc gcacattcct tctgtcggat
    12301 cttggcgtcg gtaaacgcct tccctagcgc ttccacgaca gcgccggaat ccatcacctg
    12361 accgtcgaca atcacttgcg gtccgagcgg cacgtagccc atgcggatca gccccaagcc
    12421 gcgccgggtc tcacgtacct ggcacacctt gatggaagcc gagccaatgt cgacgccgac
    12481 caggttcttt ccttcgccca tgatttggtg cggacagagc cgcgcagaaa ctctggcagg
    12541 aagcggaggt gatagccaag taacgcgcac cgggtctccg aagacccggc ataaactcta
    12601 acgatttcgc gagcccggag tctccgacgt cccggcctgg tcccccgggt cgcaacgcac
    12661 ccgcccccca ccgaacagct gtctcacgcg gctctcggag aaaacgagac tcgcgggacg
    12721 gagcgctgat caacctaccg ggtagaaaag gaccccgagc tacagaagaa atcagccaca
    12781 gatcgagaat cattggcttt tcagaccgtg gcacagccac tgcattagtc ggcgcccgga
    12841 gaccacgaaa gcccatgacg acgcgaacct cagccgcccg cgccattacc gattcggact
    12901 gttcagattc cacggaacga aaggttgctc gcccaagcgt gctgcctcgt gccggagcga
    12961 cgaaagccaa agcagcccct cggcctcgcc gtcatgagac tgccgggcgt cgcgcagccc
    13021 ggcgccccgc cgcgcagtca cctgcaacgc cggtagccat ccgtagcatc ggccgcacgg
    13081 gcctcgagct tgagtccact caagccagat tgttgctcga ggagtacacc ccgctggtcc
    13141 gcaagatcgc gggcgggttc cagcgcaagc tgccacgcaa cgtactgcgc gaggatttgc
    13201 tggccgcggg catgagtggt ttgtgggacg ccattcgccg acacggcgac acggcgggcg
    13261 agacgttcga gtggtacgtg cgcgtgcgcg tccgcggcgc gatcctcgac gagctgcgcg
    13321 ctcaagattg gctgcctcgt cgtgcgcgtg ccgccgcggc gacggcttct gccgagagcg
    13381 gcgaatacgt tccgccgccc gccgtcgtgc gcttggacga cgtgagcgag tgggagcaat
    13441 cccgttgctt gagcgacatg agctcgagtg aaacggcggt cgccgccaag ttcacgcacg
    13501 agacgctcgc caaggccgtc gagctcctgc ccgagcgcga gcgccacatc gtctcgcaac
    13561 actacttccg cggagtgaag ttcaaagacc taggcgccga gctcggcgtg agcgagcccc
    13621 gcatctcgca gctgcactcg cgcgccatgc gccgcttgaa gaccttgatg gccgaggctc
    13681 aacaaatttg agccacgtca cggactgaga ttttctgcgg atttgagctc ttttgcgcgt
    13741 cggtctccct ctggtcgcaa aagaatcgcg tctgtctgta ccagaacgtc cggggtgacg
    13801 aaaagtcgcc ccggaaccgg ttccaggcgc tttgtttgac aggctgaaag gggcttcggc
    13861 aactcctctc atgcggtacg ctgcgcgggc acaccgtgct gtaacgtgcg ctcgagagga
    13921 acatgaaacc gaccctatct gctttgttga ttaccgcagc ttttgcgggt tgttcgaact
    13981 cgaacactgg ctctgtgccc cccggggctc ccccctcgaa ctccacgggc ggagccaccg
    14041 caggcagcag ccccgtcgcg accggaggca actcagttgc gtctacgagt ggcggcacca
    14101 tcagcgcagc tggcggcgct accagtgcaa cgggcggcgc agtcagtgca tctggcggcg
    14161 ctagcgcggc cggggggagc agcac
//
LOCUS       DASTKU010000654         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_794433, whole genome shotgun sequence.
ACCESSION   DASTKU010000654 DASTKU010000000
VERSION     DASTKU010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794433"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(602..1801)
                     /locus_tag="VFQ61_22835"
     CDS             complement(602..1801)
                     /locus_tag="VFQ61_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HET9957360.1"
                     /translation="MTTKATMALAELSEKGAEVDLLREMIRHVAQRMMDMEVENLCAA
                     GYGERSPERVNSRNGYRERQWDTRTGTIDLQIPKLRKGSYFPGFLEPRRTAEKALAAV
                     IQEAYVHGVSTRSVDELVKAMGMGGISKSQVSRLCAEIDERVNAFLNRPIEGDWPYLW
                     IDATYLKVREAGRIVSVAAIIAVAVNTEGVREVLGMAIGASEAEPFWTSFLRSLTRRG
                     LRGVKLVISDAHEGLKAAAAKVLKATWQRCRVHFLRNALAHAGKGQRQMVLALINTVF
                     AQDSPESAITQWRAVADQLRTKFPKLAALMDEAESDVLAFMSFPKSHRVQIHSTNPLE
                     RLNAEIKRRTNVVGIFPNEAAITRLVGALLLEQNDEWQLQRRYLSLEGLQALADNQPA
                     RLSAVVS"
BASE COUNT          373 a          674 c          653 g          401 t
ORIGIN      
        1 catatcggag gcctcggcga gacccctggc atggccgttg cttatcttac gggcagaagg
       61 gcggcgcgac ccccgcccgg aaggaagaga cgctagcgga tatctaaaga agaattgcga
      121 cagcccgaac ggaatgactc cccgcacggg ctcacgcgat ccgacaacaa caacgacaac
      181 gacgacactc ctccacaaaa caagcaagcg accaccccct gcacaacagg gggttttttt
      241 tgcctgtgtg gcgggtgcgc gcggcttgcg cacgcccgga gcacacgggg acgtactgag
      301 ccgagcgcgc gcaagatgac gagtttcggc ccgggatgtg gcgtcgcgtg taagcccgaa
      361 gggtccgcga tctggtcgcg gcgccggatc gccctacttg aaggacgacg gtgatcgcgg
      421 catccgccgc tctccacggc gcaggcctaa tgggatgctc gcgcggtgcg gtactgcgtc
      481 ggatgtgttg cgcgcgcatc gggttcatat cggtacagaa gggctgtgcg gcgcgtgggc
      541 gcggatcgtg ccccaggacg tggtgtacga gtcgtgagtc ttggctcggt tggactcgtg
      601 ctcagctcac cactgcggag agccgcgcgg gctgattgtc agcgagtgcc tgcagacctt
      661 caagcgacag gtagcgtcgt tgcagttgcc actcgtcgtt ttgctcgagc agcagcgctc
      721 cgacgagccg ggtaatggcg gcctcgttgg ggaagatccc gacgacgttc gtacgtcgct
      781 tgatctccgc attgagccgc tccagcgggt tggtgctatg gatctgcacg cgatgagact
      841 tggggaaact catgaacgcg agcacatccg attcagcctc gtccatcagc gcagcgagct
      901 ttgggaactt cgttcgcaat tggtcggcca ctgctcgcca ttgtgtgatc gccgactcgg
      961 ggctgtcctg cgcaaacacg gtgttgatca gcgcgagaac catctggcgc tgtcccttgc
     1021 cggcatgggc cagcgcgttg cgcaggaaat ggactcgaca gcgctgccag gtggccttga
     1081 gcactttggc cgcagcggct ttcaggcctt cgtgcgcatc ggagatcacc agtttcaccc
     1141 cacgcagtcc gcgccgcgtg agcgagcgca ggaagctcgt ccagaacggc tccgcctcgc
     1201 ttgcgccgat ggccatgccg agcacttcac gcaccccctc ggtgttcacg gctacggcga
     1261 ttattgcggc caccgagacg atgcggccgg cctcgcgtac cttcaggtag gtggcgtcga
     1321 tccacaggta cggccagtct ccttcgatgg gccggttcag aaacgcattc acgcgctcgt
     1381 cgatctcggc acacagtcgc gagacctggc tcttgctgat cccacccatg cccatcgcct
     1441 tgacgagctc gtccaccgaa cgggtggaga ctccgtgcac ataagcctcc tggatcaccg
     1501 cggcgagcgc cttctccgcc gtgcggcgcg gttccagaaa accggggaaa tagctgcctt
     1561 tgcgcagctt cgggatctgc agatcgatcg tgccggtgcg cgtgtcccat tgccgctcgc
     1621 gatagccgtt gcggctgttc acccgctccg ggcttcgctc gccgtagccc gccgcgcaca
     1681 ggttctccac ctccatgtcc atcattcgct gcgcaacgtg gcggatcatc tcgcgcagca
     1741 agtcaacctc agcgcccttc tcggaaagct ctgcaagtgc catagtggcc ttggtggtca
     1801 tcgtgaaccc tcttggtcat ggttgtcggt ctggtaaccc aaccttaacc gagaactcac
     1861 ggtgaccgcc tcgaagactc cgcggccctt cgggaccgcg tctcccctac ggcttccgag
     1921 cagcaaagct cggatcagaa aaaccctcgt acaccactcc cggggacgtc agttgcgcat
     1981 cagatcgagg agtgcggggc tgagtgtcgg cttcgagtcc atcgaacctc tggccttgtg
     2041 tttggcggtg cggccatata ctgccatcgg catacgctga aacgaaaatg ccgccgagtg
     2101 c
//
LOCUS       DASTKU010000655         5629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_794456, whole genome shotgun sequence.
ACCESSION   DASTKU010000655 DASTKU010000000
VERSION     DASTKU010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794456"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFQ61_22840"
     CDS             <1..655
                     /locus_tag="VFQ61_22840"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9957361.1"
                     /translation="PGPRERPLVEAARKHHLAVALIAPDESFRGTVTLRSDNIGVGRD
                     IAQHLSELGHKRVAYVGGPEESLDSRERCEGLEQGLASFKIKLPTSNRVFQGRYHVAE
                     GVNYAEKWLKLPRSRAPTAVVLANDAMALGFMRTLQAQGVSIPKDVSVIGFDDIPAAS
                     LVWPGLTTVRQELRQMGSQACRALMSSLDQEKLERDFVEFPMKLVVRQSTGPVPELV"
     gene            1015..2172
                     /locus_tag="VFQ61_22845"
     CDS             1015..2172
                     /locus_tag="VFQ61_22845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET9957362.1"
                     /translation="MLRLPSSLAESLELGHPWVYRNHVPAGIELRSGTVVQVQCGAFQ
                     GYALWDETSPIALRILSRGTQPDRAWLRERLLEALALRASVLSHETTAARIVFGEGDG
                     IPGLSVDLYDKFAVIITYADALDRFVADVAAILTEELALEGVLLRNRELQEGEGKVKL
                     LSGRAAPERLIVREHGVKLWVELYAGQKTGMFLDHRENRAYVRGIAAGKRVLNLFSYT
                     GAFSVNAALGGATQVISVDIASGALHAAAENFTLNEVPRDLHQEIAQDVFEYLNSQRA
                     APTFDLVICDPPSFASSQKQKFAALRAYQRLNALGLSVVQPGGLYAAASCTAQVSPED
                     FRYVLSESAARAGVRFQIIHEAGQPQDHPIGIGHPEGRYLKFVVGRVLRHC"
     gene            2382..5180
                     /locus_tag="VFQ61_22850"
     CDS             2382..5180
                     /locus_tag="VFQ61_22850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957363.1"
                     /translation="MASDRTPAGPRAARGLSRSGKHFTAAAVVAILVFGVFLAYAFLA
                     RATYQSTAQFVVEPASPAATPALPAPLEAARRVHEAVLGPEQMQRLARELSPHGGVEV
                     RMLRDAFEVDTLDGRTFAVSFGDSEPERAQRLCSMLVHQAVGVAPLALATRSEEERSG
                     ETEREQRLAALMAFLSKHPEVTAGDKEGAAGKPEDAGLSALRIERERLSRRLQALESN
                     SDNPYTDPSDLNWKPDAIRRRIAEIDGVVASRHRAIGERKADATQAPPAMVDEWRRLV
                     QALSDAPPAIEPSTALKARLLSDATLPGWPVAPNRKLILLIGVLAGLGAFGIILVARG
                     GSSRRSSSGPSTRRSSELKSTAEAKSSSSDAPAGLGPMAATGLQALAGAAPGSSSPPG
                     PEGASAALPGVAAGASPALTLGIELPSVDSPALAFKVTQPFAPFLPGGAQQPGGGRPE
                     ASNIDGVAPVQTLVLEPAPGPIPPPEKTPIVATEARVLSPADGAASFPARPSPTNSAG
                     TETLMGPSAVPPPRQTQPSILPHAVIEINAQNQRAADPRRAEAAPQGQAIPQAQAVPQ
                     AQAAPGQVPTAQATPAAAQSFPENREGGAQPAQPTRPPARVTQVLGSPMQALLSELGA
                     EVRPGAGPARADAPRAERTSVPAEMLGGSGYRMSGAAAREDHAPKPHTAPSDGAAYGQ
                     PHGGSSGAPRGRASYTPPGGYAAGASYGAPPGRSGAAAPRVRLLVQDISSMFEPDSAL
                     SYESRSDLTAKVLSFGRAGQCFVTGISAAHDSSDAKSRLAVELALALAHQGRRTLLIE
                     GDFQRPSVHRLLRVNPTVSTGFSQQLHQRMRNQSLGQWTVVRCSRTLDVLPEGVMRSP
                     GLMASPQFIGCLAELREHYEFILIDGPPLSAEAECRILNSVAEGMVVVSAVASPAVLE
                     RASQLFPEKPFSAIVAAA"
     gene            5225..>5629
                     /locus_tag="VFQ61_22855"
     CDS             5225..>5629
                     /locus_tag="VFQ61_22855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957364.1"
                     /translation="MLRDVTRALNEPYYGVSLKDWLITGGIAISVAVVLRVMGGAALR
                     RTRRWAEATPTKVDDLLVTVLSSLKFLFLLAIGVWIAAHYIELSKRAEQGLRVVVSLI
                     TLLQIGISVQAGLKTFASSVSAEKGGEARTMAA"
BASE COUNT          928 a         1766 c         1891 g         1044 t
ORIGIN      
        1 ccctggccca cgcgagcgac cgctcgtgga ggcggcgcgg aagcaccatc tggcggtagc
       61 gttgatcgcc cccgacgagt ctttccgagg cacggtgacc ttgcgcagtg acaatatcgg
      121 cgtgggtcgg gacattgccc agcacttatc ggagctcggc cacaagcgtg tcgcttacgt
      181 gggagggccg gaagagtcac tggacagccg cgaacgctgt gagggcctag agcagggcct
      241 cgcctcattc aaaatcaagc tgcccacctc gaatcgagtg tttcaaggtc ggtaccacgt
      301 ggctgaaggc gtcaattacg cggagaaatg gctcaagctg ccccgctcgc gcgcacccac
      361 ggccgtggtg ttggccaatg acgccatggc gctcggcttc atgcgaacgc tccaggcaca
      421 gggggtctcc attccaaagg acgtcagcgt gatcgggttc gacgacatcc cggcggcgag
      481 cctcgtgtgg ccggggctga ccactgttcg tcaagaattg cgacaaatgg gttctcaggc
      541 ctgtcgagcc ctcatgagct cgctcgacca ggagaaattg gagcgcgatt tcgtcgaatt
      601 cccgatgaag ctggtggtgc gccagagcac tggccctgtc ccggaactag tttgagcggc
      661 tcgtcgggat tcgtgaactc gcgatggccc gggccggagg catccatgag gtttcggagg
      721 agtgctgagg gcccgcgcct gagttagtgt gcgccgccta tggctggcgc ccgtttccgt
      781 tcagatcgct cccgtcgtgg ttcagggttc gaagggcccg cgccgcgagg ctcgagccat
      841 tcgcaggaca aatccaaggg caagcacgcg acatcaccgc gagcaataac gccgggtgga
      901 ggagcgccgc gatcgcgcgt ctcgtcgcat gtcagcgcga acgcgccaag cggcgcgcgc
      961 agtgacgcga agcgagcgga acctcattcc cgctcgcatt cgggagccgc gtcggtgttg
     1021 cgtttgccga gctcactcgc ggagtcgctg gaacttggcc atccctgggt atatcggaac
     1081 catgtgcccg cagggatcga gctgcgcagt ggcacggtcg tccaggtgca gtgcggagct
     1141 tttcagggct acgcgctttg ggatgaaact tcccctatcg ctctacgcat cttgtcccgt
     1201 ggaactcagc cggatcgggc gtggttgcgg gagcgcttgc tcgaggcgct tgctttacgc
     1261 gcttccgtgc tgagccacga aactaccgcg gcgcggatcg tgtttggcga aggcgacgga
     1321 atcccaggtc tcagcgtcga cttgtacgac aagttcgcgg tcatcatcac ctacgcggac
     1381 gcgttggaca ggttcgtggc ggacgtcgcc gccattctga ccgaagaact cgcgttggag
     1441 ggcgtgctgt tgcgcaatcg ggagctccaa gaaggtgagg gcaaggtcaa gttgctctcg
     1501 ggccgcgcgg ctcccgagcg actgatcgtc agggagcatg gcgtgaagct gtgggtggag
     1561 ctgtatgccg gtcaaaagac gggtatgttc ctggaccatc gcgaaaaccg cgcatatgtc
     1621 cgcggcatcg cggccggcaa gcgtgtgctc aatctgttct cgtacacggg cgcgttttcg
     1681 gtgaacgcgg ctttgggcgg ggctacccag gtaatcagcg tggacatcgc gagtggcgcg
     1741 ctgcacgcgg ccgccgagaa cttcactttg aacgaggtcc ctcgggacct gcatcaggaa
     1801 atcgcgcagg acgtattcga gtatttgaac tcacagcgcg cagcccccac cttcgatctc
     1861 gtcatctgcg atccgccgag cttcgcgagc tctcagaaac agaagtttgc tgcgcttcgg
     1921 gcctaccagc ggctgaacgc gctcgggttg tccgtggttc agcccggtgg gttgtatgcc
     1981 gcggcgagct gcaccgcgca ggtttctccg gaagacttcc ggtatgtcct ttcggagtcg
     2041 gctgcgcgcg ccggagttcg ttttcaaatc atccacgaag ccggacaacc acaagaccat
     2101 cccattggca tcggtcatcc cgagggcaga tacctgaagt tcgtggtggg tcgcgtgctc
     2161 cgccactgct gagtcggggc cagttgaatg ggtatgtggc gtgaggctgc ccgagctagc
     2221 aatcttgcta ggtatttatt cctagattcg acgtgccggt tcgattgggc cgattcccgc
     2281 acgctggccg acgggagcgc gtcggacttt cacgcaagag tgcgcggaga gtttctgtag
     2341 atacccgtcg cgcaggtaag atgctatgcg agaactttcg tatggcatcc gataggaccc
     2401 ccgccggccc ccgggctgcc cgcggtttga gccgctcggg taaacacttc accgctgctg
     2461 cagtcgtagc gattctcgtg ttcggggtgt ttctcgcgta cgcgtttctg gcgcgtgcta
     2521 cctaccagtc cacagcgcag ttcgtggtgg agccagcgtc acctgctgcc actccggcac
     2581 tgccagcgcc cttggaagcc gctcgccgcg tgcacgaggc ggtgctcggc cccgagcaaa
     2641 tgcaacgtct ggcgcgtgag ctctcgcccc atggcggcgt cgaagtacgg atgttgcggg
     2701 atgccttcga ggtggacacg ctggatggtc gtaccttcgc ggtgtccttc ggcgactccg
     2761 agccggagcg agcgcagcgc ctgtgcagca tgctggtgca tcaagccgtc ggtgtcgctc
     2821 cgttggcgtt ggcgacgcgc agcgaagaag agcgcagcgg cgagacggag cgagagcagc
     2881 gcttggcggc gttgatggcg ttcctctcca agcaccccga ggtcacggcg ggtgacaagg
     2941 aaggggctgc aggcaaaccc gaagacgcgg gcttgtccgc gctgcgcatc gaacgcgagc
     3001 gactgtcccg ccgattgcag gcgctggagt cgaattcgga caatccgtat acggaccctt
     3061 ccgacctgaa ctggaagcca gacgcgattc gccgtcgcat cgcggagatc gacggcgtag
     3121 tggcttctcg tcaccgggcc atcggagagc gtaaggcgga cgcgactcaa gctccgcctg
     3181 cgatggtgga tgaatggcgg cgcttggttc aggcgctgtc cgatgcaccg cctgccatcg
     3241 agccgagtac tgcgctgaag gcccgcctac tgtctgacgc cacgcttccg ggctggcccg
     3301 ttgcgcccaa tcgcaagctc attctgctga ttggagtgct cgcggggctc ggcgcgttcg
     3361 ggatcatctt ggtggctcgg gggggatcgt cgcgtcgatc gagctctggc ccgagtaccc
     3421 gtcgctcgtc ggagttaaag agcactgcag aggcgaagtc ctcgtcttcg gatgcgccgg
     3481 cggggctggg gccgatggca gcgacagggc ttcaggcttt ggcgggggcg gcgccgggat
     3541 cttcatcccc gccgggaccc gagggcgctt ccgcagcttt gcccggcgtc gcggcggggg
     3601 cttcgccggc tctgactctt ggcatcgagc tgccgagcgt agattcgccc gcgctcgctt
     3661 tcaaagtgac gcagccgttc gctccgttcc tccccggagg ggcgcagcaa ccgggcggcg
     3721 ggcgtcccga agcttcgaac atcgacggcg tcgcgccagt tcaaaccctc gtgctagagc
     3781 ccgcacccgg gcctatccca ccccctgaaa agaccccgat cgttgccacc gaagcccgag
     3841 tgctgagccc ggccgacggc gccgcgtctt ttccggcgcg tccgtcgcct actaactccg
     3901 cgggcaccga gacgttgatg gggccctcgg cggttccgcc accgcggcaa acccaacctt
     3961 ccatccttcc ccacgcggtg atcgagatta acgcgcagaa tcaacgcgcc gcggatcccc
     4021 gccgggccga ggccgcgccg caaggtcagg cgattcccca agcgcaagct gttccgcaag
     4081 cgcaagccgc accagggcag gtcccaacgg ctcaggccac tccggcggcg gctcagtcat
     4141 ttccggagaa tcgtgagggt ggcgctcaac cggctcagcc cacgcgtccc cccgcgcgcg
     4201 tgacgcaggt tctgggatcg ccgatgcaag cgctcctgtc tgagctcggc gccgaggttc
     4261 gtcccggggc gggccccgcg cgcgcggatg cgccgcgtgc agagcgaacc agtgtacctg
     4321 ccgagatgct cggcgggtcc ggatatcgca tgtcaggtgc cgcagctcgc gaggaccacg
     4381 ctcccaagcc gcacaccgct cccagtgacg gggcagcgta cggccaacct cacggcggtt
     4441 ccagcggagc cccgcgcggg cgagcgagct acacgcctcc cggtggctac gccgcggggg
     4501 caagctacgg agctccgccc gggcgatctg gagctgctgc gccgcgcgtg cggctgttgg
     4561 tgcaggacat ctcgtcgatg ttcgagccag acagcgctct ttcgtacgaa tctcgctcgg
     4621 atctgaccgc caaagttttg agcttcggtc gagcgggtca gtgtttcgtc accgggattt
     4681 cggctgccca cgacagctcg gacgcgaagt cccgcttggc ggtggagctc gcgctggcac
     4741 tcgcgcatca gggccgccgc accctactga ttgaggggga ttttcaacgc cccagcgtgc
     4801 accgcttgtt gcgcgtgaac cccaccgtgt cgacgggttt ctctcagcaa ctgcaccagc
     4861 gcatgcgcaa ccaatctttg ggtcaatgga ccgtggtgcg ctgctcccgt acgttggatg
     4921 tcttgccgga aggcgtaatg cgctcacctg gtttgatggc atcgccacag ttcatcgggt
     4981 gcctggcaga gttgcgcgag cattacgagt tcattttgat cgacggcccg ccgctttcgg
     5041 cagaggcgga gtgccgcatc ctgaattcgg tggcggaggg catggtcgtg gtcagcgccg
     5101 tcgcgagccc ggcggtgttg gagcgggcct cgcagctctt tcccgagaag ccattttcgg
     5161 cgatcgttgc cgcggcgtag gcgcaaccac gggcctgccc gcgtaaacag aaggtattcg
     5221 tatcgtgctg agagacgtga cgcgcgcgct gaacgagcct tactacggcg tcagcctcaa
     5281 agattggtta atcaccggcg gcattgcgat cagcgtggcg gtggtgctgc gcgtgatggg
     5341 gggcgcggcg ctgcgccgca cgagaaggtg ggctgaagct acgccgacca aagtggacga
     5401 cttgctggtg accgtgctct ccagcctgaa gttcctgttt ctgctcgcga tcggcgtctg
     5461 gatcgcggcc cattacattg agctctccaa gcgagccgag caggggctgc gcgtggtggt
     5521 ctcgctcatc acgttgcttc agatcggcat cagcgtgcaa gcgggcctca agaccttcgc
     5581 cagcagcgtg agcgcggaga agggcggcga agcgcgcacc atggcggcg
//
LOCUS       DASTKU010000656         5720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_794477, whole genome shotgun sequence.
ACCESSION   DASTKU010000656 DASTKU010000000
VERSION     DASTKU010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794477"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFQ61_22860"
     CDS             <1..636
                     /locus_tag="VFQ61_22860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957365.1"
                     /translation="EWSHLSDGSRACLLVADDRKGLLAQVTRAFSAFQFDITSAQIFC
                     RTRADGSVEAVDFFWVRAAPGSHPSGASIEHVQERLISLLSRAEPVSQVGSSPGNLFG
                     AGQPSQPVVSSATTRVYFEPEALAVGQSVLVVETADYPGLLFAITNALFRTKVEVVAS
                     DVRTSGGVARDRFTVTNTDGRPLTGARLKEVCDVVETSIRKVNPRSNGRSN"
     gene            1099..1467
                     /gene="rbfA"
                     /locus_tag="VFQ61_22865"
     CDS             1099..1467
                     /gene="rbfA"
                     /locus_tag="VFQ61_22865"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET9957366.1"
                     /translation="MGNSEYRGKRREPDLSSGFRSLRLEQLFLEELNSILDGEISDRR
                     FDGARVSRVEISRDGASARVWVALTAPGEAQRARDVVSAFERAQRFFRSRLCDALELK
                     RTPELTFKYDPTYLLQIDPE"
     gene            1476..2678
                     /locus_tag="VFQ61_22870"
     CDS             1476..2678
                     /locus_tag="VFQ61_22870"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HET9957367.1"
                     /translation="MASAIIGGGRVPIRLWASPHEVDSDALKQLRALANLPWVAHHVA
                     VMPDVHVGKGATVGSVVALEGAVAPAAVGVDIGCGMAAMKTNLDADRLPDDLRRLRLK
                     IEAAVPVGRAQHRSAVWEQASPALRTECEALFSSMSELAPTARSITQSAHNQLGTLGG
                     GNHFIELCLDQSSQQQSSHVWLMLHTGSRRVGAALAEYHMEVASRLIHNQSLPDPELA
                     VFLAGSRAFDAYRNDLFWAQRYAALNRSLILERIKAVLSAAFPDIQFEPPIACHHNYV
                     AEEHHFGTDVLVTRKGAIRAGLGELGIIPGSMGAKSFIVRGLGNPLSFESASHGAGRR
                     LSRGQARKAFSVRDLARQTEGIECRKDHGVIDEAPKAYKNIDRVMEQQRDLVEIVAEL
                     KQVICVKG"
     gene            complement(2725..3963)
                     /locus_tag="VFQ61_22875"
     CDS             complement(2725..3963)
                     /locus_tag="VFQ61_22875"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9957368.1"
                     /translation="MTRPPCQASGTRTPTPPSIPLSKLKGRAHRARYVVSLVLPLWSL
                     AGVGCSKKSTPSPANTSVSSAALGSASAGPGTSEPLKIGFAYVGPVGDAGWTFAHDRG
                     RKEIEAEFAGKVKTTFVENVPEAADAERVFRDLVDQGNRLIFGTTFGYMEPILSVASE
                     AKDVRFEHATGYKTSGNVRTYDARTYEGAYLAGVIAGAMTKTNTLGVVASIPIPEVIR
                     NIDSFTLGAQSMNAKVSTKVVWVNKWFDPPKEAEAAQALLNQGVDVLMQNTDSSAVLQ
                     TAEKAGKFAFGWDSDMTKYGPAAHLASAIINWGPYYKKAVRDALEGTWKTEQSWWGLK
                     DGAIDLVGVSEKVPPEVKQKLQSLKSGLKEGSFTIWKGPISDQAGKEMLAGGQVADDK
                     FLRGINVYVKGVEGKLPTGK"
     gene            complement(4047..4982)
                     /locus_tag="VFQ61_22880"
     CDS             complement(4047..4982)
                     /locus_tag="VFQ61_22880"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9957369.1"
                     /translation="MNDLALLMGASIDSGTALALAGLGLLINERAGVVNLGAEGLMLV
                     AAVTGFATAVHTGSTAAALLVGGIAAAALALCFGLLVVGLNTNQYATGLALSLFGSGF
                     SAFIGLGYTQESLAQRLGGKASSTWFAGLAELPGVGPVLFGHHPMAYGAVALSALVAW
                     FLFRTRAGLALRAIGERPEVAHALGYPVRRIRLVAVTAGGALCGLAGAYVSVIYTPLW
                     VEGMIAGRGWIALALTSFATWRPARVLLGAYLFGGVTMLQLHLQGRGVNLPVQLLTSL
                     PYVSTILVLVLIARRPSWIRANMPASLGKPFHPHA"
     gene            complement(4992..>5720)
                     /locus_tag="VFQ61_22885"
     CDS             complement(4992..>5720)
                     /locus_tag="VFQ61_22885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9957370.1"
                     /translation="TLGGAVWGALPAWLRARFGVSELLVSLMLVYIAEQLLSYLVFGP
                     WKDPQGYNFPQTPMFAEAARIPRLLAAFGRLRVNLGLPLAGLTCLGSWLYLSRTYAGY
                     RLLVSGLSPRAATYAGFSSQRTVWTAFLVSGALAGLAGALEVAGPVGQLTPHVPMGYG
                     FAAITVAFVGRLHPVGVTLAALLTSMFYIGGELAQSRLGLPKSLTGVFQGLLLFALLA
                     ADTLVQYRVRWWGFRKTAPAETRA"
BASE COUNT         1097 a         1794 c         1795 g         1034 t
ORIGIN      
        1 gagtggagcc acctatctga cgggagtcga gcctgtttgc tcgtagccga cgaccggaaa
       61 ggtttgctgg ctcaagtgac gagggcattt tcagcgtttc agttcgacat tacgagcgcg
      121 cagatcttct gccgcacgcg cgcggatggc agcgtggaag cggtggactt cttctgggtg
      181 cgcgccgcgc ccggcagcca tcccagtggc gcttcgatcg agcacgtgca ggagcggctc
      241 atctcactac tcagtcgcgc tgagcccgtt tcgcaggtgg ggtcctctcc tggaaacttg
      301 tttggtgcgg gtcaaccatc ccaaccggtc gtctcgagcg ccacgacgcg cgtgtacttc
      361 gagcccgagg cgttggcggt ggggcagtcg gtcttggtgg tcgagacggc ggactatccg
      421 gggttgctgt ttgcgatcac gaacgcgcta ttccgcacca aggtcgaggt cgtcgcttcc
      481 gacgtgcgta cgagcggggg tgtggcacgc gatcgtttta cggtcacgaa caccgatggt
      541 cgcccgctta ccggagcgcg tttgaaggaa gtctgtgatg tcgtggagac ctcgattcgc
      601 aaggtcaacc cacggagcaa cggccgctcc aactagccaa gttctgaatt cccgctccac
      661 tctcgatctt cggctcgggc gtgcatatgg cagtttgttc acggaaaata gcgcgaccac
      721 attgacaagc ggcgcgtgaa ctgtcagttt tcgcgcctcc gcaggcgtga accggacgct
      781 gctcttacgg gcctgcgaga aaggagatga tgccgatgcc ccgacgaccc gaaaaacacg
      841 ttgaacgagt cttcatcgag tcatcatctc caatcatttc aatttgagtc ggcatcttag
      901 tgcgtgctga aaccgggcct cgccgaggcg caggtttcgt ggtgcgctct gagtccggcc
      961 tgccatgatc gcaatcgaac acgcattcac gaggatgcgc tgcgcctcga cgggccgttg
     1021 acggcccaac acgggagcac gcaagctcgt gagccgcgcg gtttcggccg tgcatgtgtg
     1081 ttcacgaatg gatgccaaat gggcaattca gaatatcgcg gcaagcgtcg tgaaccagat
     1141 ctatcctctg gattccgaag cttgcgatta gaacagctgt ttctcgaaga gttgaattcc
     1201 atattggacg gcgagatcag tgaccgtcgt ttcgacggcg ctcgcgtcag tcgtgtagag
     1261 atatcgcgtg atggagcgag tgcccgcgtt tgggtcgcgc tcacggcgcc cggcgaagct
     1321 caacgcgcta gggatgtcgt gtctgcgttc gaacgcgcgc agcgcttctt tcggtcgcgc
     1381 ttgtgtgacg cgctcgagct gaagcgcacc cccgagctga ccttcaaata cgatccgaca
     1441 tacctattgc agatcgatcc ggagtgaggc ccaacatggc cagtgcaatc atcgggggcg
     1501 gacgggttcc gatccgcctc tgggcatccc cgcatgaagt ggattcggat gccttgaagc
     1561 agctgcgcgc gctcgcgaac ctgccgtggg tggcgcatca cgtggccgtc atgcccgacg
     1621 tccacgtcgg gaagggagcc accgttggtt cggtggtggc tctagaagga gcggtggccc
     1681 ccgccgcagt cggagtcgat attggctgcg gtatggccgc catgaagacc aatttggacg
     1741 ccgaccgttt gccggacgat ttgagacgtc tgaggctaaa aatcgaagct gcggtgccgg
     1801 tggggagagc acagcatcgc tcagcggtgt gggagcaagc gagcccggcg ctccgaaccg
     1861 aatgcgaggc tttgttttcg agcatgagcg agcttgcgcc caccgcccgc agcatcaccc
     1921 aaagtgcaca caatcagctc ggcacgctcg gtggaggcaa tcacttcatc gagctatgcc
     1981 tggaccagag ctcgcagcaa cagagctcgc acgtctggct gatgttgcac accggtagcc
     2041 gtcgagtggg cgcggccttg gccgagtacc atatggaagt cgcttcccgc ctgatccaca
     2101 accagagcct gccagacccc gagctagcgg tgtttttggc gggcagccgc gccttcgatg
     2161 cgtatcggaa cgacctgttc tgggcgcagc gttacgcagc tctgaatcgc agcttgatcc
     2221 tggaacgcat caaggccgtg ctcagcgcgg cttttccaga cattcagttc gaaccgccca
     2281 tcgcctgtca ccacaactac gttgccgaag agcaccactt cggcaccgac gtgctcgtga
     2341 cccgcaaggg ggcgattcgc gcggggctcg gtgaactcgg catcatcccg ggttcgatgg
     2401 gggcgaagtc gttcatcgtg cgaggtctgg gcaatccgct ctcgttcgag tccgcttcgc
     2461 acggggcggg tcggcggttg agccgaggtc aggcacgcaa ggcgttcagc gttcgagatc
     2521 tggcgcggca gaccgaaggc atcgaatgtc ggaaggacca cggcgtgatc gatgaggccc
     2581 cgaaggccta caagaacatc gaccgcgtca tggagcagca gcgagatctc gtcgagatcg
     2641 tggccgagct caagcaggtg atttgtgtga agggttgaac ggtgggggcc cgagccggtc
     2701 cgaaactggc ttgggctcgg gtcctcactt gcccgtgggg agtttcccct ccacaccctt
     2761 cacgtagacg ttgatgccac gcaggaactt gtcgtccgcg acttggccgc cggccagcat
     2821 ttccttgcca gcttggtccg agattggccc cttccaaatc gtgaacgagc cctccttcaa
     2881 gccgctcttc aggctctgga gcttctgctt cacctcgggc ggcacctttt cggacacgcc
     2941 gaccaggtcg atggcgccat ccttcaggcc ccaccaggat tgctcggttt tccacgtgcc
     3001 ttcgagcgcg tcccgcacgg ctttcttgta gtacggcccc caattgatga tggcggacgc
     3061 gaggtgtgcg gcggggccgt acttggtcat gtccgagtcc cacccgaacg cgaacttgcc
     3121 ggctttttcg gccgtttgca gcacggccga tgaatcggtg ttttgcatca gcacgtcgac
     3181 gccttggttc aaaagcgcct gggccgcctc ggcctccttg ggcgggtcga accacttgtt
     3241 gacccagacc accttggtcg acaccttggc gttcatgctt tgggccccca gggtaaagga
     3301 gtcgatattg cggatcacct ccgggatagg gatcgacgcc accacaccga gcgtgttcgt
     3361 cttggtcatc gcgcccgcta tgacgccggc taagtaggcc ccctcgtaag ttcgtgcgtc
     3421 gtaggtgcgc acgttgcccg atgtcttgta gccggtcgcg tgctcgaaac ggacgtcttt
     3481 ggcttcggat gccacggaca ggatgggttc catgtagccg aaggtcgtgc cgaaaatcag
     3541 gcgattgccc tggtcgacga gatctcgaaa aacgcgctcg gcgtctgcgg cttcggggac
     3601 gttttcgacg aacgtcgtct tcactttacc cgcgaactcg gcctcgattt ctttgcggcc
     3661 gcgatcgtga gcgaacgtcc agcccgcgtc acctaccggg cctacgtaag cgaagccgat
     3721 cttgagcggc tccgaggtgc cgggtcccgc tgaggcgctg cccaaggccg cgctgctcac
     3781 gctcgtgttc gcagggctcg gagtcgactt cttactgcag ccgacgcccg cgagggacca
     3841 gagcggaagg accaaggaca cgacgtaacg agcgcggtgt gctcgtccct tgagcttcga
     3901 aagcggaatg gacgggggcg ttggggtgcg ggtgccggaa gcctgacagg gcggacgagt
     3961 catctcggga gatctcctca atgaaagcgc gttggttctt cgagccgtgg agtgtcatgc
     4021 gttggagcgg gtcgcgcatg gagggttcaa gcatggggat ggaagggttt gcccagcgac
     4081 gccggcatgt tggcgcgaat ccagctcggt cggcgagcga tcagcaccaa taccagaatc
     4141 gtggagacgt acggcaagct cgtcaggagt tggaccggca agtttacacc tcgaccttgc
     4201 agatgcagct gcagcatcgt cactcccccg aacagataag cgccgagcaa tactcgtgcg
     4261 gggcgccaag tggcaaacga ggtcaaggcc agcgcaatcc agcctcgccc ggcgatcatg
     4321 ccttccaccc acaagggcgt gtagatcacc gacacataag caccggcgag cccgcacaat
     4381 gctccgcctg ccgtcactgc gaccaagcgg attcggcgca ccggataccc cagcgcgtgc
     4441 gcgacctccg gtcgctctcc aatcgcgcgc agcgcgagcc ccgcgcgcgt gcgaaacagg
     4501 aaccaagcca ccaaggcgct gagggccacc gctccataag ccatgggatg atgtccgaac
     4561 agcaccgggc ccactccggg cagctcggcg agcccggcaa accatgtcga agatgccttc
     4621 ccgccgagcc gctgggcgag gctctcttgc gtgtacccga gcccgatgaa ggcggaaaag
     4681 ccagatccga acaagctcag cgccagcccg gtggcgtact gattggtgtt gagcccaacg
     4741 acgagcagcc cgaagcagag tgcgagcgca gccgccgcga tgcccccgac cagcagcgca
     4801 gccgcagtgc tgccggtgtg cactgcggta gcaaaaccag tcactgcggc caccaacatt
     4861 agaccctcgg cacccagatt cacgacaccc gcgcgctcgt tgatcagcag cccgagcccg
     4921 gccagggcca aggcggtccc tgaatcgatc gaggcgccca tcagcaaagc gagatcattc
     4981 atgcggcgcc ttcaggctcg agtctccgcg ggggcggtct tgcgaaagcc ccaccatcgc
     5041 acgcggtatt gcacgagcgt gtccgccgcg agcaatgcga acagcagcag accttggaat
     5101 acgccggtca aagacttggg cagccccaat cgcgactgcg cgagctcccc accgatatag
     5161 aacatactgg tcaagagcgc ggcgagcgtg accccgacgg gatgcagccg acctacgaaa
     5221 gccaccgtaa tcgccgcgaa tccatagccc atcggcacgt gtggcgtgag ctgcccaacg
     5281 ggcccggcga cttccaaggc ccccgcaagc ccagccaaag cgcctgacac gaggaaggcc
     5341 gtccacaccg tgcgttgcga cgagaagccc gcatacgttg cggcgcgagg cgagagcccg
     5401 ctcaccaaca acctataacc cgcgtacgtt cgcgagagat acagccacga acccaggcac
     5461 gtaaggcccg ccagcggcag ccccaaattc acgcgcagcc tcccgaatgc tgcgagcagg
     5521 cgcggaatgc gcgccgcctc cgcgaacatc ggcgtctgcg ggaaattgta gccctgggga
     5581 tctttccaag gcccaaacac caagtagctc aagagctgct cggcgatata gaccagcatc
     5641 aagctcacga ggagctcgct gacaccgaac cgtgcccgca gccaagccgg cagcgcgccc
     5701 cagaccgctc cgccgagtgt
//
LOCUS       DASTKU010000657         3365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1912451, whole genome shotgun sequence.
ACCESSION   DASTKU010000657 DASTKU010000000
VERSION     DASTKU010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912451"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..761
                     /locus_tag="VFQ61_22890"
     CDS             195..761
                     /locus_tag="VFQ61_22890"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET9957371.1"
                     /translation="MQQTNGALTLDSPLEVTAGNLEPDSQSSELLDHFLSLLGKFDTA
                     ILVTHQGDHGLHGRPMAIADTDADGTLWFLTSDDSPKVAEIAEDSRVLVTMQQDTRFI
                     VASGRIRVVRDAKKIDELWKESDRVWFDGKDDPNLVLLSFFPEDAEFWDNSGSRGVRF
                     VYRAAKAVLSGVPLKDLNDPTAHAKVSL"
     gene            814..1758
                     /locus_tag="VFQ61_22895"
     CDS             814..1758
                     /locus_tag="VFQ61_22895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET9957372.1"
                     /translation="MPPIHSIAPVSTWVLFAALIIAFIAFDLGVLQRKSHEVSLREAG
                     ALSVMWITVAALFGCGVYFYAGRDSALEFATGYLLEKALAVDNLFVFALIFNHLRLPP
                     RYQHRVLVWGVLGAIVLRALFIAAGSLFVAYFSWALYVFGVLLVILGIRLAFASEKTG
                     DLEESSLLRALRRVLPTTTRIHGRDFWAREDGKLRATPLFVGLVFVELCDVLFALDSL
                     PAIFAVTHDPFLVFSSNILAILGLRALYFLLAHLLDRFKYLRYGLALTLCLIGAKLLL
                     KDVIHLPVIVTLAVTLSTIVGATLISFLVVRRTEPEPS"
     gene            complement(1825..>3365)
                     /locus_tag="VFQ61_22900"
     CDS             complement(1825..>3365)
                     /locus_tag="VFQ61_22900"
                     /inference="COORDINATES: protein motif:HMM:NF016972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HET9957373.1"
                     /translation="LFTWIVAENGGLLYSPSTHEELALGPEPPPALLALLKARGVGPI
                     SAGRTIIATWEPHQATVLECIHELGLDLQVIFNKNAVMILPAGVNKRTGLAAALARLD
                     ASPHNCVGIGDAENDHALLSFCECGVAVENALPALKQAADWVTNGDHGAGVCELITHL
                     LASDLALIDPQLTRHHILVGKDAHGHDLTLAPYGTLALIAGGSGAGKTTATTGIIERF
                     AENKYQVCVIDPEGDHSELPEALVSGTPEQPPDLEQVLRMLAKPSENVVVNLIGVPLA
                     DRPRFFISLLGRLQELRASTGRPHWLVVDEAHHVLDGAFEPALLTLPSELQSMLLVTV
                     HPERLPELVLRQVQWVLSIGEQAGATLSAFAKAAHKPTPLVAATPERPGEALLWRTMS
                     HNADMIQLVPGRWVHRRHKRKYARGDLGPDKSFVFRGPHGALNLRAQNLVAFNEIGAG
                     VDDATWDHHLKLGHYSSWIREHIKDEGLAERVRAVEIDDDVDAQASRARIRQMIEDAY
                     TNPA"
BASE COUNT          580 a         1040 c         1044 g          701 t
ORIGIN      
        1 aagttggggc gcgtggctga gtgctccagt aaccagatct gtaaagggta gccctcgctg
       61 agccaccgct cgcggcgggc ccaggcaagg cgcgcggtgt cttcggactc gcgcgccttg
      121 cctgctttat ccgtggccct cgagtgtctg cagcggctgg aattgctggc atatgggttg
      181 cagaaattcc caccatgcaa cagaccaatg gcgctctcac tctagattct cctctcgaag
      241 tcactgcggg caacctcgaa ccggattcgc agtcttccga gctgctggat cacttcctga
      301 gtttgcttgg aaaattcgac acagccattt tggtaaccca ccagggagac cacggccttc
      361 acggccgccc catggcgatc gccgacaccg acgcggatgg caccctctgg ttcctgacca
      421 gcgacgattc gccaaaggtc gcggagattg ccgaagattc ccgcgtgctc gtcacgatgc
      481 agcaagacac tcgtttcatc gtcgcgagcg gtcgcatccg agtggtgcga gacgccaaga
      541 agatcgatga gctctggaag gagagcgacc gagtctggtt cgacggtaaa gacgatccga
      601 acttggttct gctgagcttc ttcccagagg acgcggagtt ctgggacaac agcggctcgc
      661 ggggcgtgcg cttcgtgtac cgtgccgcca aagccgtact gagtggtgtg ccgttgaagg
      721 atctcaacga tcccaccgcg cacgcgaaag tctcactcta gccccgtgct tcgctggtcc
      781 gatcgtcgcc ctcagcccta gatcgccccc aatatgccgc cgatccacag tattgctccg
      841 gtcagcacct gggtcctgtt cgccgctctg atcatcgcgt tcatcgcgtt cgatctcggc
      901 gtgctacagc ggaagtccca cgaggtctcg ttgcgcgagg cgggggcgct gagcgtgatg
      961 tggatcaccg tggcggccct gttcgggtgc ggagtgtact tctatgcggg gcgagacagc
     1021 gctctggagt tcgcaaccgg ctatttgttg gagaaggcgc tcgccgtcga caatctgttc
     1081 gtcttcgctt tgatcttcaa tcacctgcgg ctgccgccgc gctatcagca tcgcgtactc
     1141 gtttggggcg tgttaggagc gatcgtgctg cgagcgttgt tcatcgcggc gggcagcctg
     1201 ttcgttgctt acttcagctg ggccttgtac gtcttcgggg tgctgctggt catccttggt
     1261 atacggttgg cgtttgcctc cgaaaagaca ggcgacctcg aggagagtag cttactgcgg
     1321 gcgctgcgcc gcgtgctgcc gactacgaca cgcatccacg gacgggattt ctgggcgcgc
     1381 gaggacggta agctgcgcgc cacgccactg ttcgttgggc tcgtgttcgt cgagctctgc
     1441 gatgtcctgt ttgcgctgga ctcgctgccg gccatctttg ccgtgactca cgatccgttc
     1501 ttggtgttca gctcgaacat tctggccatc cttggtctgc gcgcgctgta cttcctgctc
     1561 gcccatctgc tcgaccggtt caagtacctc cgttatggat tggcgctcac gttgtgcctc
     1621 atcggcgcca agttgctatt gaaggacgtc atccaccttc ccgtcatcgt gacgctagct
     1681 gtcacgctca gcaccatcgt gggcgcgacg ttgatctcct tcctggtcgt gcgccgcacg
     1741 gagccggaac cgtcctgagt tacgaaggcg cgagcggcac cggaagcgga aactgctagc
     1801 gtcgttaccg acgaaggccg gcaatcatgc cgggttcgta taggcgtctt cgatcatctg
     1861 acgaatgcgg gcacggctcg cctgggcatc cacatcgtcg tcgatctcca cggcgcgcac
     1921 tcgttcagcc aggccttcgt ccttgatgtg ctcccggatc cagctggagt agtgtccgag
     1981 cttcaggtga tggtcccagg tggcgtcgtc tacgccggcc ccaatctcgt tgaacgcgac
     2041 caagttctgc gcgcgtagat tgagcgcacc gtgtggacct ctgaagacga agctcttgtc
     2101 gggacccaag tctcctcgag cgtacttgcg cttgtgccgc cggtgaaccc accggcctgg
     2161 aacgagttga atcatatcgg cgttatgcga catcgttcgc cacaatagcg cctcaccggg
     2221 gcgctcaggt gtcgcagcca ccaatggcgt gggcttgtgg gcggccttgg cgaaagccga
     2281 aagggtagcg cccgcctgtt cgccgatcga aagcacccat tgaacctgac ggagaacgag
     2341 ctccggcaga cgctccggat gaaccgtgac caacagcatg gactgtagct ccgaaggcaa
     2401 ggttagtagc gccggttcga acgccccgtc gaggacatgg tgggcttcat cgaccacgag
     2461 ccagtgaggc cgcccggtgc tggcgcgcag ctcttgcaaa cggcctaaca ggctaatgaa
     2521 gaagcgtggg cgatccgcga gtggaacacc gattagattg accaccacgt tctcgctcgg
     2581 cttggcaagc atgcgcagga cttgctccag atcgggtggt tgctcgggag tccccgagac
     2641 caacgcttct ggcagctccg agtgatcgcc ctccggatcg atgacgcaga cttgatattt
     2701 gttctccgcg aagcgctcga tgattccggt ggtggcggtg gtctttcctg cgcccgagcc
     2761 acccgcgatc agcgcgagcg tgccgtacgg cgcgagcgtg aggtcgtgtc cgtgcgcgtc
     2821 cttgccgacc agaatgtgat gacgggtcaa ctgaggatcg atcagggcca gatcactcgc
     2881 caatagatgg gtgatcagct cgcagacgcc ggcgccgtgg tcgccattcg tgacccagtc
     2941 ggcagcttgc ttcaaggccg gcaaagcgtt ttcgacggcc accccacact cgcagaaact
     3001 gagcaaggcg tggtcgttct cggcgtcgcc gatgcccacg caattgtgcg gcgaagcatc
     3061 caagcgggcc agcgccgccg cgagcccagt gcgcttgttt acccccgcgg gcaggatcat
     3121 caccgcgttc ttgttgaaga tcacctggag atcgaggccg agctcgtgga tgcactcgag
     3181 cacggtggcc tggtgcggct cccaggtcgc gataatcgtg cgtcctgcgc tgatcgggcc
     3241 caccccccgc gccttcagca gcgcaagtag ggctggcggt ggctccgggc cgagcgccag
     3301 ctcttcatgc gtgctcggtg aatagagcaa gccgccgttc tccgcgacga tccaagtgaa
     3361 tagat
//
LOCUS       DASTKU010000658         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_571642, whole genome shotgun sequence.
ACCESSION   DASTKU010000658 DASTKU010000000
VERSION     DASTKU010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571642"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFQ61_22905"
     CDS             <1..310
                     /locus_tag="VFQ61_22905"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HET9957374.1"
                     /translation="DGSEPPRAPNIPGGDTWRPVLAAAFERHCAEIERDVPTLLGGYA
                     ATNDSEFFAVATETFFEKPAELQAVYPDLYQQLAMLFRQDPARNLRPEDHAAKVSFGR
                     "
     gene            complement(345..632)
                     /locus_tag="VFQ61_22910"
     CDS             complement(345..632)
                     /locus_tag="VFQ61_22910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957375.1"
                     /translation="MAAVVEPGSPREDPEGAEVGHEERVSLLGTGEALERGAVEDGAA
                     GKGPLEVHGRDLDRLVDAEQIGELQEERADAPSAELVKDLLFRHLAQRQGL"
     gene            602..1531
                     /locus_tag="VFQ61_22915"
     CDS             602..1531
                     /locus_tag="VFQ61_22915"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET9957376.1"
                     /translation="MGTRARPRQPRSHRADRAPRLRHPLSRRPPLRRLPAHCPQAPAR
                     ARPQRRLCDGGRLRGGVLRPGARHLAHAADDLATLRVVVRNVALRYGLVATFMPKPVF
                     RFSGSGLHTHQALSANGKNAFTDPDAPLQLSDVARWYIGGLLRHARGNCAVTNPLVNS
                     YKRLVGGFEAPVNVTWSTQNVSPLIRVPAARGESTRCENRMPDPAANPYLALLVQLAA
                     GLDGIHEKTDPGDPVNKNIARMSYRERRKYRIDDLPRDLHEALDHLERDRVVRDALGE
                     HIYERYVDAKREEWEEYIGRVSPWEVERYMGQY"
     gene            2099..>2433
                     /locus_tag="VFQ61_22920"
     CDS             2099..>2433
                     /locus_tag="VFQ61_22920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957377.1"
                     /translation="MPPDTGLSEIEMMTLVYDYIGVDGGYLFGFSYNSHEEFYPRYCN
                     IRIDVARLRKEHGTTRNTFLEILRNAAPSLQAQIVEGTFQFLPLEKFPNESREAKTGA
                     KAKLLAAVAR"
BASE COUNT          430 a          793 c          759 g          451 t
ORIGIN      
        1 cgatgggagc gagccaccac gggcgccgaa catcccgggc ggtgacacat ggcgcccggt
       61 gctcgccgcc gccttcgagc gccattgtgc cgagatcgag cgcgacgtcc ccacgctcct
      121 cggcggctac gcggcgacga acgacagcga gttctttgcg gtggcaaccg agacgttctt
      181 cgagaagccg gccgagctac aggcggtcta tccggatctg taccaacagc tggcaatgct
      241 ctttcgccag gaccccgcgc gcaaccttcg ccccgaggac cacgccgcaa aagtatcctt
      301 tgggcgttga cagtcagagc tggatttcaa atcgtagacg tgagtcacag cccctgacgc
      361 tgggccaaat gccggaacaa gagatccttg acgagctccg cagacggggc atccgctcgc
      421 tcctcctgca gttcaccgat ctgctcggcg tcaacaaggc ggtcgaggtc ccgtccatgc
      481 acttcgagcg ggcccttgcc ggcggcacca tcttcgacgg ctccgcgctc gagggcttcg
      541 cccgtaccga ggaggctgac gcgctcctcg tgcccgacct cggcaccttc cgggtcttct
      601 cgtggggacc cgggctcgac cacggcagcc acgatcccac cgagctgatc gcgcgcctcg
      661 tctgcgacat ccgctatccc gacggccgcc ccttcgaagg ctgcccgcgc actgccctca
      721 agcgccagct cgagctcgcc cgcaacgccg gctatgtgat gggggtcggc tccgaggtgg
      781 agttcttcgt cctggagccc gtcacctcgc ccacgccgcc gacgacctcg ccacgctccg
      841 cgtggtggtg cgcaacgtgg cgctccgcta cgggctggtg gccaccttca tgcccaagcc
      901 ggtgttccgg ttcagcgggt ccggcctcca cacccaccag gcgctctccg ccaacgggaa
      961 gaacgccttc accgaccccg acgccccgct gcagctctcc gacgtcgccc gctggtacat
     1021 tggcggcctg ctccggcacg cccgcggcaa ctgcgcggtc accaatccgc tggtgaactc
     1081 ctacaagcgg ctggtgggcg gcttcgaggc gccggtcaac gtcacctggt ccacgcagaa
     1141 cgtctcgccg ctcatccgcg tccccgcggc ccgcggggag agcacccgct gtgagaaccg
     1201 catgcccgat ccggcggcga atccctacct cgcactgctg gtgcagctcg cggccgggct
     1261 cgacggcatc cacgagaaga ccgatcccgg cgacccggtc aataagaaca ttgcgcgcat
     1321 gagctaccgc gagcgccgca agtaccggat cgacgacctc ccccgcgacc tgcacgaggc
     1381 gttggaccac ctcgagcgcg accgggtggt gcgcgacgcg ctcggcgagc acatctatga
     1441 gcgctacgta gatgcgaagc gcgaggagtg ggaggagtat atcgggcgcg tgagtccgtg
     1501 ggaggtcgag cgctacatgg gacagtactg atccgagcaa gaagtcttgc cggggcgggt
     1561 gctcgatcaa tttcaagtcg tcgacgtact cgccgggtgc ggcatggcga gcctctagcg
     1621 ggctcgcggc ctggggacca gccgtatcgg cgattaagtt gaccaaggct ccgctctcag
     1681 catattagcg cttgtcggaa tgcctccggc atggcgtgtc aagtgcgtca atccttgaag
     1741 tgtattgacg caatcgaggc aagtcgcgtt tctaccgcga cacgaacaca acgtcacgtt
     1801 gggctgatgc cgtcgtggcg ggctcgacgc gtcgctgagg ggagcgcatc ttcaggtgtg
     1861 tcgcccgtct gcccctttgg atatctagtc tgaggctgtc aggtcaatgc agtccattgc
     1921 gtaattgggc cgatatagcg acccgcagtt cagcatgtgc cttcgatgag acagtgtgtc
     1981 ggtcgcgttg gcgtgagttc ttggtggttg ccattcgttg caagcggatg tgtgcgcgag
     2041 cttgaccgct gcgatcagtg tcgatgggac tggttgctta catggggcag ggtggcgcat
     2101 gccgccagac acaggactga gcgaaattga aatgatgact ctcgtctacg actatatcgg
     2161 tgtggacggg ggctacctat ttgggttctc gtacaattcg cacgaggagt tctaccctcg
     2221 atattgcaac atcagaattg acgttgctcg acttcgcaag gaacatggca ctacacgcaa
     2281 cacgttcctc gaaatccttc gaaatgcggc accgtcgctt caagcgcaaa ttgtcgaggg
     2341 gacgtttcag tttctccctc ttgaaaagtt tccaaatgag tctcgtgagg cgaagacggg
     2401 cgccaaggcc aagttgctgg cagctgtggc tcg
//
LOCUS       DASTKU010000659         7743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1017926, whole genome shotgun sequence.
ACCESSION   DASTKU010000659 DASTKU010000000
VERSION     DASTKU010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017926"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFQ61_22925"
     CDS             complement(<1..464)
                     /locus_tag="VFQ61_22925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9957378.1"
                     /translation="MDPLLLDLSVIVPVYNEEESLPHLVSELHAALEPTGLSYELVLV
                     DDGSVDGSYALLERMARLDPSLHVVRFRRNFGQTAAMQAGLDASRGRKVVTIDADLQN
                     DPADIPAMLAKLDEGYDMVAGWRKNRQDAFVNRKLPSMIANRIISLATRVKL"
     gene            complement(615..1685)
                     /locus_tag="VFQ61_22930"
     CDS             complement(615..1685)
                     /locus_tag="VFQ61_22930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957379.1"
                     /translation="MSEPEPNPPHVEPEPLAGPRLAWSKHPELARWSRRLGVLTTLAA
                     VAFLGLRLHGELVTLLSARGADRVAQLGVGGLDATIPKLIAASVACLASFSLAVLNWS
                     NLLPAPERITLPRLGVIYGYTLVTKYLPGGVFQYLFGVRVAERLGLRKSTMVQLFLRD
                     SLLAIPTGVALAALSLLGPARAWGTRALDQHGPRWIISLLVFAALLGSLFVLGSRFAV
                     FAKLRAAGRNLLDLSPRVLGAAVLLNALHFFAHGVAARQLAELWAPEPLAPLPSSFSL
                     SCGFALAWLVGLLVPGAPSGLGVREVLLTSLFASSLGEARAILSFVSLRVVSCACELL
                     FFAPAPWLERRYRVLQKPLDKP"
     gene            1848..2738
                     /locus_tag="VFQ61_22935"
     CDS             1848..2738
                     /locus_tag="VFQ61_22935"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HET9957380.1"
                     /translation="MSRSVVRVGLVQMSMGLDKAENVKKAQARIQEAAAKGAEIVCLP
                     ELFASQYFCQSEDASTFDLAEPIPGPSIQGIAEAARAARVHVVTPVFERRAPGVYHNS
                     LVLLSDAGQITGHYRKMHIPDDPLFYEKYYFAPGDLGFTCFETPRAKVGPLICWDQWY
                     PEAARLTALTGADVLVYPTAIGWHPAEKAEYGAAQLSAWQTIQRSHAIANGVFVVAVN
                     RVGHEGPADGGIEFWGHSFVADPFGIVLAEASEKEETLVVECDLSRMEFVRRNWPFLR
                     DRRIDAYQDITKRFIDPSRR"
     gene            2952..4022
                     /locus_tag="VFQ61_22940"
     CDS             2952..4022
                     /locus_tag="VFQ61_22940"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HET9957381.1"
                     /translation="MPAEWEPHAATWLAFPHEISDWPGKFGPVPWVFAEIARALAPGE
                     RVRLIVNDAKLEKVARQTFERAGVPLTEVDFIRSETNRSWTRDFVPSFVVRGKRPRRA
                     GSLGGRQRDGIAAVKWRFNGWARYKNHAADEQTGQRVARWLDVPCFEPRSHVGRREDR
                     IVLEGGAIDVDGEGTLLTPEECLLDGRQARNPRLGAHGHEQILLENLGIERVIWLERG
                     IAGDDTGGHVDDFARFVAPGVVVLAQEKNKRDPNHRILEAARERLEGARDARGRRLRV
                     LRVPMPAPLLFDGQRLPASYANFYVGNAAVLVPTFNDPLDRTALGILSEAFPGRRVVG
                     IHALDLVLGLGTIHCSTQQEPL"
     gene            4109..5221
                     /locus_tag="VFQ61_22945"
     CDS             4109..5221
                     /locus_tag="VFQ61_22945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957382.1"
                     /translation="MPLSSPFPRSVDWTPRLRRGLAAARSLSLTIALLLAITQGTARL
                     AAAAPSAGAAGVGGAPPAAAAKKSPEPVAKAANGDPVVAKPPPWTQKQKTSAERGVNP
                     CNTPDPGFGSYSGWNREVSIGQMIAPKQGAVNARGEFDVMIHFHGHEPARKEWVQVMQ
                     RGVLVGIDLGLGSGPYESTFQPRDAFRTLLQSIEKAVAKHSGKPNAHVRRIGLSAWSA
                     GYGAIQSILSDPWAMEHVDTVVLLDGLHCGYRGPTLNALQIQPFIEFAKLAAASKRLM
                     VVSHSSIIPPGYASTTETANFLVAKVGGKPERARPRKNDPMGLELISRYSRGNFHVRG
                     FSGNDKMDHCAHLGFFRDVLRAHVVPRWSSRIVQTR"
     gene            5205..6134
                     /locus_tag="VFQ61_22950"
     CDS             5205..6134
                     /locus_tag="VFQ61_22950"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET9957383.1"
                     /translation="MSKPAESHYVVPRELADAPLDRTLRGWLADKSWNDVRQFISTGK
                     VRVDGRVELDSRTRVQPGQRIEYVANAPRPRAPGVSKSALVYVDSQVVVVEKPAHVSS
                     VAFDENERNTLDEQVARLLAELERQKRPPLGVVHRLDKETTGLLVYARTLPAKRSLKN
                     QFRFHTVKRRYYAICEGHLPSQTLASRLVQDRGDGRRGSTENPELGREAITHVRLLER
                     LAGASLLECRLETGRTHQIRIHLSEVGHPLIGERVYRRNKAEDRQGGGTRVMLHAFEL
                     GFEHPSSGKPLHFESPMPEDMAQLYERLRQPRR"
     gene            6246..>7743
                     /locus_tag="VFQ61_22955"
     CDS             6246..>7743
                     /locus_tag="VFQ61_22955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957384.1"
                     /translation="MSNLADRGRRLGAQGLRSYLTRAASSSREVLSNAVGQLRTGKGV
                     RALGLTCVLLIAAGFFLGFVDRSYPIREWLFFRYAGYWAVVLVWGAGSFGVGYGLLTR
                     VFRVRFGLLTEALLSFTLGQLAFEYLLFLVGLCQGYRAFAFYLVPLAFLALGGGGLLA
                     LLRRTQRILARPAPPRSLLRFATLAFGCAALGMIYFLILTPDNVQFDARWKHMALAED
                     YAAHGGIHRSPEGWMFAARPHITSYLYAWAFLIPSGRLFDQMLLGAHLEYFAFLVSTV
                     LGIPALVRRLVPRADPAVVWAARFLFPGVLLYDSSLSGGTDHFGALYAIPIAIVLLRS
                     WRDFTARTLSLLATLLAGPVLVKETAALLLVPFPLLVVGTRFVTDLWQRLRTRSASDG
                     PSATLSPARPFWLAPLVAIGTGLLVTTPLWLKNLVWYGDPLYPSLHRFFPSHPWSEAA
                     AYKLKWAYSERQMWAPKHDLQGLIATLKALFTYSFVPNNWKALHRDVPV"
BASE COUNT         1356 a         2435 c         2590 g         1362 t
ORIGIN      
        1 tgcaacttga ctcgagtggc caagctgatg atgcgattgg cgatcatcga ggggagcttg
       61 cgattgacga acgcgtcttg gcggttcttt cgccagcccg cgaccatgtc gtagccctcg
      121 tcgagcttgg cgagcatggc gggaatgtcc gccggatcgt tttggagatc tgcgtcgatg
      181 gtgacgacct tacgaccgcg cgaggcgtcg agccccgcct gcatcgcggc ggtttgcccg
      241 aagttgcgcc ggaagcgcac gacgtgcagg ctcggatcga ggcgcgccat gcgctcgagc
      301 agggcgtagc tgccgtcgac agagccgtcg tcgacgagca ccaattcata agacagcccc
      361 gtgggttcga gcgccgcgtg gagctccgac acgaggtgcg ggaggctctc ctcctcgttg
      421 tagacaggga cgatgactga aagatccaag agtaaggggt ccacgcgcga tccggctctc
      481 cttgccacga gcgggcgacg ccccctttac cacgctagcg ctgcgtgtcc ggccgatttg
      541 gcagttggcc aatgagccag tagacacgga cggtcgctaa gtccgtccac ctgcgagagg
      601 gtcgcagccg aaggttacgg tttatcgaga ggtttttgca gaacgcgata acggcgttcc
      661 aaccagggcg caggggcaaa gaacaacaat tcgcaggcgc aagagaccac gcgcaggctc
      721 acgaagctga ggatggccct cgcctcgccg aggctcgaag caaaaagact ggtcagcaat
      781 acctcgcgca cgccgagccc ggaaggtgcg ccggggacga gcaggccaac tagccaagcc
      841 agcgcgaagc cgcagctcag gctgaacgag gagggaagcg gcgcgagggg ctcgggcgcc
      901 caaagctcgg cgagctgccg ggcggccact ccatgcgcaa agaaatgcag cgcattgagc
      961 aggaccgcgg cgccgagcac ccttgggctg aggtcgagca ggttgcgacc ggcggcgcgc
     1021 agcttcgcga agacagcaaa acgagagccg agaacgaaga gggagccgag cagagcggcg
     1081 aacacgagca ggctgatgat ccagcgtggg ccgtgctggt ctagagcgcg cgttccccag
     1141 gcccgcgcgg ggccgagcag gctgagagca gccaaggcca cgccggtggg gatggccagc
     1201 agcgagtcgc gtaggaagag ctgcaccatg gtgctctttc ggagtcccaa acgctccgcc
     1261 acgcggactc cgaagagata ttgaaagacg ccgcccggca ggtacttggt cacgagcgtg
     1321 tagccataaa taacgccgag ccgcggcaac gtgatgcgct cgggcgcggg gagcaggttg
     1381 ctccaattca agacggcgag cgagaaggat gccaagcacg caacgctcgc cgcgatcaac
     1441 ttggggatcg tggcatccag accgccgacg ccgagctgag ccactcggtc cgcgccccgt
     1501 gcgctcagta gcgtaaccag ctcgccgtgc aggcgcagcc cgaggaaggc cacggcggcc
     1561 agcgtcgtca atacgcccag tcgacgagac caacgcgcca gctcgggatg cttcgaccac
     1621 gcgagtcgtg gcccggcgag cggctcgggc tcgacgtgcg gcggattagg ctcgggttcg
     1681 gacatggccg cgtacgcctc agtacgttga acggtggctg gtccgccaca gagaatttac
     1741 ggaaggctcc gcgggtgcca gggacggcgg gcgctcgggc tcgcgcgccc caacttcgcg
     1801 ggtctcgctc ctggtttcat cgcgtaggtt gttctaggct gccggccatg agtcgctccg
     1861 tcgttcgcgt tggcctcgtg cagatgtcca tggggctcga caaagccgaa aacgtcaaga
     1921 aggcccaggc gcgcatccaa gaggcggccg ccaagggcgc cgagatcgtg tgtttgcccg
     1981 agctctttgc ctctcagtac ttctgccaat ccgaagacgc gagcacgttc gacctggccg
     2041 agccgatccc cgggccgtcg atccaaggca ttgccgaggc cgcacgcgcg gcgcgcgtgc
     2101 acgtggtcac tccggtgttc gagcggcggg cgccgggtgt gtaccacaac agcttggtgc
     2161 tgctcagtga tgcgggccaa atcaccgggc actatcgcaa gatgcacatt ccggatgatc
     2221 cgctgtttta cgaaaagtat tacttcgcgc cgggagattt gggctttacg tgcttcgaga
     2281 caccgcgcgc caaggtgggt ccgctgattt gctgggacca gtggtatcca gaagccgcgc
     2341 gcctgaccgc gctgacgggc gccgacgtgc tggtgtatcc gaccgcgatc ggttggcatc
     2401 ctgctgaaaa ggccgagtac ggggcggcgc agctctcggc ttggcagacg attcagcgct
     2461 cgcacgcgat cgcgaacggc gtgttcgtgg tggcggtgaa tcgcgtcggc cacgaagggc
     2521 cggccgatgg cggaatcgag ttctgggggc actcgttcgt cgccgatccg ttcggtatcg
     2581 tgctggccga ggcatccgag aaggaagaga cgctggtggt ggagtgcgat ctatcgcgca
     2641 tggagttcgt gcggcgcaac tggcccttcc tgcgggatcg gcggatcgac gcctaccagg
     2701 acatcaccaa gcgcttcatc gatccgagcc gccgctgatc gctgacccgc gtcactcacc
     2761 ctccgcatcg cgtttggaac gaagagaatt cgccagtgaa aaagcgttcg tcgaagcgca
     2821 cttcagcacc gaagagcacg agccccgccc gatccgcact cggccctgca aaggccagcg
     2881 cggctcgtgg cgcgaacctc aatccagagg ccgcccccgc aacggagaca cctcgcgccc
     2941 gaggctaccg aatgcccgcc gagtgggaac cgcacgcggc cacctggctc gcctttcccc
     3001 acgagatcag cgattggccc ggcaagttcg ggccggtgcc gtgggtcttc gcggagattg
     3061 cgcgggcgct cgcgccggga gagcgcgtcc gcttgatcgt gaatgacgcc aagctcgaga
     3121 aggtggcacg ccaaaccttc gagcgtgcgg gcgtgccgct cacggaagtg gacttcattc
     3181 gtagcgagac caatcgctcc tggacccgtg atttcgtgcc cagcttcgtg gttcgcggca
     3241 agcgcccgcg ccgcgcgggt agcttgggcg ggcggcagcg cgacggcatc gccgccgtca
     3301 aatggcgctt caatggctgg gcacgttaca aaaatcacgc cgctgacgag cagacgggcc
     3361 aacgcgtggc tcgatggctg gacgtacctt gcttcgagcc tcgctcgcac gtcgggcgcc
     3421 gtgaggatcg gatcgtgctc gaaggcggcg cgatcgacgt cgacggcgag ggtacgctgc
     3481 tcaccccgga agagtgcctg ttggatggtc gccaagcgcg caatccgcgg ctgggagcgc
     3541 acgggcacga gcagattctg ctcgaaaacc tcgggattga gcgggtgatt tggttagagc
     3601 gtggaattgc cggtgacgac accggtggtc acgtcgacga cttcgcgcgc ttcgtggcgc
     3661 ccggcgtggt ggtgctggca caagaaaaga acaagcgtga tccgaaccat cgaatcctgg
     3721 aggccgcgcg cgaacgtctg gagggtgcac gcgacgcgcg cggccgcagg ctccgagtac
     3781 tgcgtgtgcc gatgcccgcg ccgttgctgt tcgacgggca acggctgccc gcaagctacg
     3841 ccaatttcta tgtgggaaac gcggcggtgc tagtgcctac cttcaacgat ccgctcgatc
     3901 gaaccgcgct cggcatcctg tccgaggctt tcccggggcg gcgcgtggtc gggatccacg
     3961 ccctggactt ggtgctcggt ctcggaacga ttcactgcag cacccaacaa gagccgctgt
     4021 aacgacgcgc ggaaaggtgg cggcaaagag cgcgcaagaa ggcggtgctt gcgcgccctt
     4081 tgcgctgtgc caagcctgcc ggagcgccgt gcccctgagc tccccgttcc cccgctccgt
     4141 tgactggacg ccgcgtctgc ggcgcgggct cgccgccgcg cgctcactgt cgctcacgat
     4201 cgcgctgtta ctcgcgatca cgcagggcac ggccaggctc gccgcggccg caccctcggc
     4261 gggagcagcc ggcgtgggtg gcgccccacc cgctgcggcc gcgaagaaaa gtcccgagcc
     4321 cgtcgccaag gcggcgaacg gagatccggt ggtagccaag ccaccgcctt ggacccagaa
     4381 gcaaaagacc tcggccgagc gcggggtgaa tccctgcaac actcccgatc cgggattcgg
     4441 cagttacagc ggctggaatc gcgaggtctc gattgggcaa atgatcgcgc ccaagcaggg
     4501 agcagtgaac gcgcgcggcg agttcgacgt gatgattcat tttcacggtc acgagccggc
     4561 acgcaaagag tgggtgcagg tcatgcagcg cggggtgctc gtcgggatcg atctcggact
     4621 cggctcgggc ccttacgaga gcacgtttca accgcgcgac gcgttccgga ccttgctcca
     4681 gagtatcgag aaagccgtgg ccaagcacag cggcaaaccc aacgctcatg tgcggagaat
     4741 tgggctcagc gcatggagcg cgggttacgg agcgattcag agcatcttga gcgatccatg
     4801 ggccatggag cacgtggata cggtggttct gctagatggg ctgcattgcg gctatcgcgg
     4861 ccccaccctg aatgcgctgc aaattcagcc gttcatagag ttcgctaaac tcgcggcggc
     4921 ctcgaaacga ttaatggtgg tgagtcactc ctccatcatc ccgcccggct acgcatcgac
     4981 cacggagacc gccaatttcc tggtcgcgaa ggtgggtggc aagcccgagc gggcgcggcc
     5041 gcgcaagaac gatccgatgg gcctcgagtt gatctcacgc tattcgcgcg gcaacttcca
     5101 cgtccggggc ttctccggca acgacaagat ggatcactgc gcacacctgg gcttctttcg
     5161 tgacgtgctg agagcgcacg tggtgccgcg ctggagtagt cgaattgtcc aaacccgctg
     5221 aaagtcacta tgtcgtgccg cgcgagctgg ccgacgcgcc cctcgaccgc acgctacgcg
     5281 gctggctcgc agacaagagc tggaacgacg ttcgtcaatt catcagtacg ggcaaggtgc
     5341 gcgtggacgg ccgcgtcgag ctagattctc gcacgcgagt gcagccgggg cagcgaatcg
     5401 agtacgtggc caatgcgccg cggcccaggg cgcccggcgt gtcgaaatca gcgttggtat
     5461 acgtcgactc gcaagtagtg gtggtggaga agcctgccca cgtcagcagt gtcgcgttcg
     5521 acgaaaacga gcgcaatacg ctcgacgagc aggtggcgcg cctactcgcc gaactcgaac
     5581 gtcaaaagcg ccctccgctc ggcgtcgtgc atcggctcga caaggaaacg acggggttgt
     5641 tggtctacgc tcgcaccctg cccgccaaac gctcgctcaa gaaccaattt cgctttcaca
     5701 cggtgaagcg ccgctactac gcgatctgtg aagggcactt gccgtcccaa acgttggcga
     5761 gccgcctggt acaagaccgc ggagacggtc ggcgcgggtc gaccgagaac ccggagctgg
     5821 gccgtgaagc cattacgcac gtacgcctgc tggaacgcct ggcgggggcg agcctgctcg
     5881 aatgccggct cgagaccggt cggacgcacc agattcgcat ccacctctcc gaagtgggtc
     5941 accccctgat cggcgaaaga gtctatcggc gcaacaaggc cgaggaccgc caggggggcg
     6001 gaacgcgggt gatgctgcac gccttcgagc tcggcttcga gcatccctct tcgggcaagc
     6061 cgctccattt cgaatccccg atgccggaag acatggcgca gctttacgag cggctgcgcc
     6121 agccgcgacg ttaggcgacg gctgtgcggc gggtacgccg gtgcgccccg cgtcgaggct
     6181 gtccccaccg cctcttttcg gcagctttga ttcggctggc gctccgcgct aatgcggcga
     6241 ggcttatgtc caatcttgcc gatcgtggcc ggcggctcgg ggcccagggg ctccgctctt
     6301 atctaacgcg cgcggcctct tccagccgcg aagtcttgtc caacgccgta gggcaactac
     6361 gaaccggtaa gggggtgaga gcactgggtt tgacttgcgt gctcttgatc gcggcgggat
     6421 tctttttggg gttcgtggat cggagctacc cgatccgtga atggctgttc ttcaggtacg
     6481 cgggctattg ggcggtcgta ttggtttggg gcgcgggcag cttcggcgtc ggctatggct
     6541 tactgacgcg agtgttccgc gtgcgcttcg ggctgctgac cgaggccctg ctttcattca
     6601 ccttggggca gctggcattc gagtacctgc tgttcttggt cggactgtgc caggggtacc
     6661 gcgcatttgc gttctatctg gtgccactcg cattcctggc gctgggtggc gggggattgc
     6721 tggctctgct ccggcgaacg caacgcatac tggctcggcc cgcgccccct cggagtctgc
     6781 ttcgattcgc gacgctggcg ttcggctgtg cggcgctggg catgatctat tttctgattc
     6841 tgaccccgga caacgtgcag ttcgacgcgc gctggaagca tatggcgctc gcggaggact
     6901 acgcggcgca cggcggaatt caccgctcgc ccgaaggttg gatgtttgcc gctcgcccgc
     6961 acatcacgag ctatctctac gcctgggcgt tcttgattcc ttctggacgc ctgttcgatc
     7021 aaatgctgct tggggctcac ctggagtact tcgcgttcct ggtgagtacg gtgctcggga
     7081 ttcctgcgct cgtgcggcgc ttggtgccca gagcggaccc ggccgtggtc tgggccgctc
     7141 gctttttgtt tcccggggtg ctgctgtacg acagctcctt gagcggcggc accgatcatt
     7201 tcggtgccct ctacgccatt cccattgcca tcgtgctgtt gcgtagctgg cgcgacttca
     7261 cggcgcgcac cctgtcgctg ctcgcgacgc tgctcgctgg ccccgtactc gtcaaagaaa
     7321 cggccgcgct attgctggtc ccgttcccgt tgctggtcgt cggcacccgg tttgtgacgg
     7381 acctttggca aaggctccgc acgcgatctg catcggatgg accaagcgcg acgctttcgc
     7441 cggcgaggcc gttctggctc gcgcccctcg tcgccatcgg caccggacta ctcgtgacga
     7501 cgccgctctg gctcaagaac ctggtttggt atggcgaccc gctttatccg agtctgcatc
     7561 gcttcttccc ctcgcatccg tggtccgaag ccgccgctta caagctgaag tgggcttatt
     7621 cggagcgtca gatgtgggcg ccaaagcacg acctccaggg cctgatcgca accttgaaag
     7681 cgctcttcac gtactcgttc gtgccgaaca actggaaggc gttgcaccgg gacgttccgg
     7741 tgt
//
LOCUS       DASTKU010000660         4831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1018023, whole genome shotgun sequence.
ACCESSION   DASTKU010000660 DASTKU010000000
VERSION     DASTKU010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018023"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VFQ61_22960"
     CDS             complement(<1..606)
                     /locus_tag="VFQ61_22960"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9957385.1"
                     /translation="MQLDALQSLALSVAQARSPDSVLKEMVQGLGMWEGVALARVWLL
                     RDEPGQEPYLELKASIGASINDPSLRWNRIDGSHAKIPLSFGKVGHIASRNQPLLLQR
                     GPGTWLLEPKWAELEGIQSFAGQPLCFRGQVLGVVAFFSRKRLEKANLDWLRVFADHA
                     AVAIANARAFEEIERLKENAERERDYLREAVRSALHQGEIIA"
     gene            725..1948
                     /locus_tag="VFQ61_22965"
     CDS             725..1948
                     /locus_tag="VFQ61_22965"
                     /inference="COORDINATES: protein motif:HMM:NF017380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HET9957386.1"
                     /translation="MSKRAGYADLPLHGGRVPAWLSARMARLGRVITEAIVQHYGRQE
                     WLARLAHPFWFQSFGAVMGMDWHSSGITTSVLGALKRGLAPVQDELGIWVCGGRGRHS
                     RRTPSELQAIAETTGQDGDALARTSRLIAKVDSAAVQDGFDLYLHAFVLSADGHWTVV
                     QQGMMPERRLARRYHWQSAGLKSFVDDPHTAIEGQSRGMIVNLADARAESNRAAQLEL
                     SARGPDPVLAVLRKERASQERVAEPAQQVLPHLVLPSHHEVREDDVLMRRLRGTLAAA
                     ADAGAKDFADLLLVPGLGARTLEALAHVAEVVHGAPCRFTDPARFSMALGGKDGHPFP
                     VPLKIYDETLRVLRQAVDSAKLGNDERLTAIRELDRQARVLETRANTGPTFEEFVSRE
                     REASARYGGRTVLDR"
     gene            complement(1985..3457)
                     /locus_tag="VFQ61_22970"
     CDS             complement(1985..3457)
                     /locus_tag="VFQ61_22970"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HET9957387.1"
                     /translation="MNPLDRAPRRLPPHANWTIIRLTMLRKERAREVGLERLLEAVDQ
                     VQSFSSPLQEDRDLDRLIARIGDARYVLLGEASHGTTEFYTWRTRLTRRLIREKGFRF
                     VAVEGDWPECSAVNRYVRAYPDAPNSALSVLQGFQRWPSWMWANREVMTLVQWLRGHN
                     AVLPEDQRVGFYGLDVYSLWRSMSAVIGYLDRKDPEAAERVRHAYECFAPFEQDEEEY
                     ARATVLLPTSCEDDVIQALCELQAVQPSSSSSLVASGNLEFSATANARDREAWFDAEQ
                     NALVAKNAELYYRTMVRGGTASWNVRDMHMAETLDRLAEFHGPEAKAIVWEHNSHVGD
                     ARFTDMGRGGELNLGQLARERHEREGVFIVGFSTYQGTVIAGDAWGAPFKRLAVPPAR
                     PGSWDDVLHQASRGADALLLFPGADAPDVLLAPRGQRAIGVVYRPQYEQFGNYVPTVL
                     PLRYDALLFVDTSHALDPLPIPSLPRAAEKDFPETFPSGL"
     gene            3773..4057
                     /locus_tag="VFQ61_22975"
     CDS             3773..4057
                     /locus_tag="VFQ61_22975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957388.1"
                     /translation="MATATSNTANPSSKSTSGVGSPISNEAYNVITALQAKLEGLEAY
                     RKYSSNAGSSVWQELTRADVQSVELLVNELEQLVRDGKLRLREPGRADAE"
     gene            4547..>4831
                     /locus_tag="VFQ61_22980"
     CDS             4547..>4831
                     /locus_tag="VFQ61_22980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mannan endo-1,4-beta-mannosidase"
                     /protein_id="HET9957389.1"
                     /translation="MNVSGGAQNSGGTATGGSAGGGTANGGTTNSTSGGAASMGGGLS
                     TGGTSARGGASATASGGAAPAGGATANGGASPKGGATSSGGAAGGAAPMGG"
BASE COUNT          953 a         1512 c         1522 g          844 t
ORIGIN      
        1 cgcgatgatc tccccttgat gcagagcgct gcgcactgcc tcgcgcagat agtctcgttc
       61 gcgttcggcg ttctccttga gccgctcgat ctcctcgaac gcgcgtgcgt tcgcgatcgc
      121 caccgccgca tggtcggcga agacccgtag ccaatcaagg ttcgccttct cgagccgttt
      181 gcgactaaag aaggccacca cacccagcac ctgcccacga aagcagagcg gctgtcccgc
      241 gaagctttgt atgccttcga gctccgccca cttcggttcg agcagccaag tgccgggccc
      301 gcgctgcaaa agcagcggct ggttgcgcga tgcgatgtga ccaactttgc caaagctgag
      361 cgggatcttg gcgtggcttc cgtcaattcg attccagcgg aggctgggat cattgatgga
      421 agctccgatg ctggccttga gctctagata gggctcctgg ccgggctcgt cacgtaacaa
      481 ccagacgcgg gcgagcgcca ctccttccca catgcccaac ccctgcacca tctctttgag
      541 taccgagtcg ggagagcgcg cttgcgcaac cgaaagagcc aaggactgga gcgcatcgag
      601 ctgcatccgt agcggcactc cacaccgcgg ccggctacgg gcgtgtgtcc accgagtttc
      661 cgagcggacg cggaacggcc agggccgcgt gcaaaaggcg gcgttcgacg ctagagctct
      721 cgaaatgagc aagagagccg gttacgccga tcttcctctg catggggggc gcgttccggc
      781 ttggctctcg gcgcgcatgg cgcgtttggg tcgggtgatc acagaggcaa tcgtgcagca
      841 ctacgggcgg caagagtggc tcgcgcggct tgcccaccca ttctggtttc agtccttcgg
      901 cgctgtgatg gggatggatt ggcactcgag tggtatcacg actagcgtcc tcggggcatt
      961 gaagcgcggc ctcgccccag tccaagacga gctcggaatt tgggtatgcg gaggtcgagg
     1021 ccggcactcg cggcgcacac cttcggagct gcaggctatc gccgaaacca cgggtcagga
     1081 cggagatgca ctcgcgcgga ccagccggct gatcgcgaag gtagattcgg cagccgttca
     1141 ggacggcttc gatctatatc tccacgcctt cgtgttgagt gctgacgggc actggaccgt
     1201 ggttcaacaa ggaatgatgc cggagcgccg cttggcacgc cgctatcact ggcagagtgc
     1261 cggactcaag agcttcgtgg acgaccctca tactgcgatc gagggccaga gccgcggaat
     1321 gatcgtgaat ttggcggacg cgcgagcgga gtcgaatcgt gccgcgcagc tcgagctatc
     1381 cgcgcgtggt cccgatccgg tgcttgccgt actccgcaaa gaaagagcct cccaggagcg
     1441 tgtggctgaa cccgcccagc aggtgctgcc acacctcgtt ctgcccagcc accacgaagt
     1501 gcgcgaagac gacgttctca tgcgccgctt gcggggcacc ttggcggcgg ccgcggacgc
     1561 aggcgccaaa gacttcgccg acctgctctt ggtaccgggt ctcggcgcgc ggacgctgga
     1621 ggccctggca catgtggccg aggtagtgca tggagcgccg tgccgcttca ccgatcccgc
     1681 ccgtttttcc atggccctcg gtggcaaaga cggtcatccc ttccccgttc cgctaaagat
     1741 ttatgacgag acattgcggg tattgcggca ggccgtcgat agcgcaaagc tcggcaatga
     1801 cgagcggctc actgcaatcc gggagctaga tcgccaggcc cgcgtgctcg aaactcgagc
     1861 caacacgggg cccacgttcg aggagttcgt ttcgcgagag cgagaagctt cggcgcgtta
     1921 cggggggcgc accgttctcg atcggtgagt gtcggcgatt cgccccgctc actccgcagc
     1981 ccgttcacag tcccgatggg aaggtctcgg gaaagtcctt ttcggccgct cgagggagcg
     2041 atggaattgg cagcgggtcg agcgcgtggc tagtgtcgac gaacagaagt gcgtcgtaac
     2101 gcagcggcag aaccgtaggt acgtagttac caaactgctc gtattgaggg cgatacacga
     2161 cgccaatcgc acgttgaccg cgcggcgcca ggagaacgtc tggcgcgtcc gccccgggga
     2221 agagaagcag ggcgtccgcc ccgcgggaag cctgatgcag aacatcgtcc cagctgcccg
     2281 ggcgagcagg gggaacggca agacgtttga acggagcgcc ccaggcgtcc cccgcgatca
     2341 ccgtgccctg gtacgtggag aaccccacga tgaatacgcc ctcccgctcg tggcgctctc
     2401 gagcgagttg cccgaggttc agctcgccgc cccgacccat atcggtgaat cgtgcgtcgc
     2461 ccacgtggct gttgtgctcc caaacgatgg ctttggcttc ggggccatga aactcggcaa
     2521 gccggtccag agtctctgcc atgtgcatgt cccggacgtt ccagctcgcg gtcccgcctc
     2581 gcaccatggt gcgatagtag agctccgcat tcttcgcgac gagcgcattc tgctccgcgt
     2641 cgaaccaagc ctcccggtcc cgagcgttgg ccgtcgccga aaactcgaga ttgccagacg
     2701 caaccagact actcgatgac gaaggttgaa cggcttgcag ctcgcacaag gcctgaatca
     2761 cgtcgtcttc gcaggaagtc ggcagcagca ccgtggcgcg agcgtactcc tcttcgtctt
     2821 gctcgaatgg ggcgaagcac tcgtaagcgt gccttacacg ctcggccgcc tcgggatcct
     2881 tgcggtccag gtatccgatt accgccgaca tcgatctcca aaggctgtac acgtccagcc
     2941 cgtagaaccc aactctttgg tcctccggca ataccgcgtt gtgccctcgc agccactgaa
     3001 cgagcgtcat tacctcacga ttggcccaca tccacgacgg ccaacgttga aagccttgca
     3061 agacggacag agcggagttc ggggcgtctg ggtaggcccg aacataacga ttgactgcag
     3121 agcactctgg ccaatccccc tcgacggcca cgaagcggaa tcccttttct ctaatgagac
     3181 gccgggtcag gcgcgttcgc cacgtgtaga actcggtcgt accgtgcgag gcctcgccca
     3241 gtagcacgta ccgggcgtcc ccgatgcgcg cgatcaagcg atccaaatca cgatcttcct
     3301 gcaacggcga agagaaagac tgcacttggt ctactgcctc tagcagcctt tcgaggccga
     3361 cctctcgagc tcgctcctta cgcaacatag tgagtctaat gatggtccag ttcgcgtgcg
     3421 ggggcagccg gcgcggcgcg cgatctagag ggttcattga agccacaccc cgcgcatgac
     3481 tgcagcgcgg agacgggagt gacaagaagg tacgaagctg aatgccacgg ctttcgagac
     3541 tcgatgcgta agagcacgac cccatgactc ggatcgccag gcatgaccgt tgcaaccgaa
     3601 cgattgcaac taagtcgctg caaccgaagt gagtgcaagc gagttacacg cccgcgaatc
     3661 cacgtgagca tgtaaagagc acatcgagtc accgcagcac ggtcgctcga cgcgacttac
     3721 ccgttagaga cgaattaccc caaaacccca attacccagt acgaggtgaa gcatggctac
     3781 tgcaacttcg aacaccgcca acccgagcag caaatcgacc agcggcgtcg gctctcccat
     3841 ctcaaatgaa gcgtacaacg tcattaccgc cctacaggcc aagctggaag gactcgaggc
     3901 gtacaggaag tacagttcga atgccggcag ctccgtttgg caagagctca cacgcgcgga
     3961 tgtgcagagc gtcgagctct tggtgaacga actcgagcag ctcgttcgcg atggcaaact
     4021 ccggcttcgc gaaccgggcc gagccgatgc tgaatgaagg gctcgcaagg gcgctgcgac
     4081 cttaaaggca cccagagttc atcgacgcgc ccgcgcccgt ttggtccacg ccggtcggta
     4141 cacgcggcgc gctcccaatg aacacgcgtg ggccgactcg gcgtagctcg agtcgaggct
     4201 cccactgggg cccgtacacc gaggagtgaa actttgcgtc tgagcgctcc ttcggagcat
     4261 cgctttcggc agtggagctc tcggaccaag caagtgggcg ggcatcttca gatgggacgg
     4321 ctttttccac tctaaacgtg gtcagaaaag tcgccgaaat gacggacctc gaactggcag
     4381 aggtgacact ggttacaaac tagtgacaat gccgagaaga atgacgattg ctgcgggttc
     4441 gttccattca ctctttgccc tcgtcgtgcc aagcgtactg ctcgcggcgg ggtgctcttc
     4501 gagcaaaaat gagggcagtc cggcaggggg tgctggaact actgcagtga acgtgagcgg
     4561 tggcgcgcag aactcgggag gcacagctac gggaggctca gccggtggtg gcacggctaa
     4621 tggcggcaca acgaacagca catcgggagg agccgcatcg atgggtggag gtctatccac
     4681 cggaggcacg agcgctcgcg gtggggcttc cgctaccgcc agcggtggcg ccgcgcccgc
     4741 cggaggcgct acggcaaacg gcggtgcgtc gcccaagggt ggcgcgacct cgagcggtgg
     4801 agcagccgga ggcgcggcgc ccatgggagg c
//
LOCUS       DASTKU010000661        11095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1242569, whole genome shotgun sequence.
ACCESSION   DASTKU010000661 DASTKU010000000
VERSION     DASTKU010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11095
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242569"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VFQ61_22985"
     CDS             complement(<1..826)
                     /locus_tag="VFQ61_22985"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9957390.1"
                     /translation="MGYGQNWHLKYLCVALACACSVDKNHNYKAGQGGESGSSGSTSG
                     GRTAGTGGGPTGGSGGRGGSSSAGSTNAGAGGVIANGGTGGSPTGGTTANPEGGTDGG
                     ETDTGGTGGSPPEPPKGEPCKAADGTYECLGTAGKTRRVCRDGYWEDGESCAASQNCD
                     SRDGSCKEIIAQCVDQTPGFRYCDASNQSLTCGPDLVSTESLACMGKCVQGQCAPVSC
                     GDGVKQAPEECDDGNMVDTDDCTSGCKNKACGDGFIQSGEACDDGNTTAGDGCTSCK"
     gene            complement(1041..2015)
                     /locus_tag="VFQ61_22990"
     CDS             complement(1041..2015)
                     /locus_tag="VFQ61_22990"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HET9957391.1"
                     /translation="MTRVLGLRLSTLLLALWSLLLAENSFAHPINSASLILKEVRHGY
                     FSMQFKSGSPTLEQQVTPAIFPKPCVRAGAYIDCGPSGMVGRIDFPWLEGASMRFMVE
                     IEWQDGARLLRVVTASAPSLTVYGGARTGWISLKPIIADYTWLGVEHILTGFDHLLFV
                     IALTLLVTGRRLLLATITAFTVAHSVTLAFTALGLLSVPTAPVEATIALSIVLVCAEC
                     LRPADSLTRRAPWLVAFAFGLLHGLGFASALLEIGLPEKHVPSALLCFNLGVELGQLA
                     VIGVILALGGFVERSNLNKQTWTRRAVYAMGGVAAFWAIDRIGVVFSG"
     gene            complement(2012..2827)
                     /locus_tag="VFQ61_22995"
     CDS             complement(2012..2827)
                     /locus_tag="VFQ61_22995"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9957392.1"
                     /translation="MPNEMSRNPVWRRLATEPVVHFFLIGALLFLAQRWVAGTPRTII
                     VTPGVRAAMARRFEDLHERKPTASELEAEVRNWKRDEALFRESLRERLDQDDPDVRAV
                     LVGKMRARAELEVRDPEPSEAELSEWLNSHRSDYESPRTYDFEFIAFPKSEPTAPEKL
                     EQFARKINEGTNPAMLGRPVIGGNLSVADIRERFDLELAERIPMLPLGAWARVESKDT
                     LFLVQVKQLGGGLPSLEQIRPGVLADWKAAMRERAIERLLQRTVERYRFEEQP"
     gene            complement(3018..4451)
                     /locus_tag="VFQ61_23000"
     CDS             complement(3018..4451)
                     /locus_tag="VFQ61_23000"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9957393.1"
                     /translation="MISLHRSFAALGVIVFAVSCSSTDPINNNTGGSPPVGTGGAKAS
                     GGTSAQTGGTSTTTGGTTQATGGAVSGGSGGGTSTSGGSSAKGGASATGGSNSGGAGG
                     STSVTGGTSAGGTSATGGAGPGSGGGGGKASGGSSSGGSGGNTGGATGMGGTTSTGGS
                     GPQGGTGPLGASGYPAPGNGGVAKPSGTPGAITVLPWAGFKGAVSYTFDDTNQTQINR
                     YAEMNALGVRFTFFMQTGKQNEFGNSVWSQAKTAGHELANHTRTHSGTASDPDIKNGQ
                     SDIQGKFQVTPVTMAAPNGTDGYADVAKNNRYLINRGVGKTNPGFNDPISSQYNLSGW
                     LPAGGNADYGSVTSAVSGGKWIIVCIHGWNDQGATDGAYQATSFSSFTSHVTSLKNSG
                     NAWIDTVENIGSYWVGSKSFAAATTTTSGSDKTWTWTLPANFPKGNYLRVKVDGGTLK
                     QNGTTLPWDPHGYYEISLDALSVTLSP"
     gene            complement(5147..6508)
                     /gene="gor"
                     /locus_tag="VFQ61_23005"
     CDS             complement(5147..6508)
                     /gene="gor"
                     /locus_tag="VFQ61_23005"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF004776.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET9957394.1"
                     /translation="MSKHGYDLLVLGGGSGGLAAARRAASYGVSAAIIEGAELGGTCV
                     NRGCVPKKIVWNLSRVLDSLHDAKGYSLGEFSPQLDYRSFKNKRDQYVARLNAIYERN
                     LGVDGVTLIRGWGKIEGPNTIRVTALDGSEQTVTGKHLLIATGSAPERPNIPGAELGE
                     TSDDFFAWETLPRRVAIVGAGYIAVELASALSHLGVDVSLIIRHERLLRRFDRTITDE
                     LIEQMTAAGVSMITGFTAQRLERTEQGLTLTSETGHVEGGYEHVIWAIGRRGRIQDLG
                     LERVGVPVENGFVITDEWQATRAVDIFALGDVTGKNELTPVAIAAGRKLADRLYGGKA
                     DARLDYTDVPTVIFSHPPVASVGLSEEEALERFEDGVKCYRTRFFDMYYGLTERRTPT
                     VMKLVTVGATEKVVGIHLIGRNCDEIIQGFAVALKMGATKADLDRTVAIHPTAAEELV
                     TMR"
     gene            complement(6556..7632)
                     /locus_tag="VFQ61_23010"
     CDS             complement(6556..7632)
                     /locus_tag="VFQ61_23010"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9957395.1"
                     /translation="MDIAALLQDRTRLTLAAQPIVDLRRGEVVGYEGLSRFQLDPPAG
                     PDRVFAEAARQGLGAELESVVVARCLELAQQKPTNCFFAMNVDPSHLLEPAVRKVLEA
                     TPDLRGIVLELTEHRAIENMKALAAELGRLRERGALVAVDDAGSGYSGLQQILALRPQ
                     ILKVDRALVMNVHEDDAKRALIQMLGELAGRLDAWLLAEGVETESELSLLRQIGVPLA
                     QGYFLARPAPPWADILPVAQRVLVNAAPSLRPARILRPLLEPCATCLSDEDWPAQNTA
                     VRLSGEGRPLGLRFLNAEQHHVRSEHELMRVKPDSALESVAQRAATRPERVRWDPLIC
                     TDDRGLFLGIVRMSRLVCALASQD"
     gene            complement(7666..7845)
                     /locus_tag="VFQ61_23015"
     CDS             complement(7666..7845)
                     /locus_tag="VFQ61_23015"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HET9957396.1"
                     /translation="MKEFSCGDVVPGCTAKFRCNSDEEVLREVAAHARDAHGIKDVPP
                     ELIKVVREHIRSVQA"
     gene            8258..8761
                     /locus_tag="VFQ61_23020"
     CDS             8258..8761
                     /locus_tag="VFQ61_23020"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HET9957397.1"
                     /translation="MPISPSGPSAPLVVPSVPDEIKVPGDATLIQRTNARGVQIYTCV
                     PKPAAPGQFEWALKAPEASLTDDAGKVLGKHYAGPTWESTDGSKVVGAMKAKVDSPSP
                     TAIPWLLIEAQSNAGEGAFSRIAFVQRVNTEGGKAPAQGCDKAHEKSEARVDYTATYY
                     FYARSKP"
     gene            8982..>11095
                     /locus_tag="VFQ61_23025"
     CDS             8982..>11095
                     /locus_tag="VFQ61_23025"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET9957398.1"
                     /translation="MDHRAAAEHSGLPQAVRLAMNAPIASTSGVARTLSRLRLPLLMC
                     VSGFASLTYQMVWTAQGALWIGHESAAVLAVITAFFGGLAVGGLAFGKLAQHSANPTR
                     WYGACELCIAAWGCILLAVGAHFQAGLQTLLESPAENGFPWLTTFLLMFLWLLPATTA
                     MGATLPAMQRLGSRRADGWHSLPELYACNTFGAVLGVLLPTFWLIPQFGFTRTLQVCI
                     ALNLACAASPLFLTNRDPTHGSSSHLGIGAITKSPEPESAWPHYITLAVTGFLGIAYE
                     VLVVRVLSQVTEDTVYTFALLLSVYLVGTAVGAGAYQRFAATSLVTPARLRIRLLGAT
                     ASACLVGTTSLWQSNWIRDTASSALGSSFPAAIATEALLAGCAFGPAAVAQGALFSHL
                     SQSLIGAGANFGVALGMNTLAGSLAPILAGVVMATALGSKASLLWVAIGYVLLARPWG
                     SARRELALWVPAACAMGLAVFAPPLSFLEVPEGGRVVSHEEGVMAAVSVVEDARGVAR
                     LSINNRQQEGSSASALVDARQAWIPLLLHPHPRRALFLGLGTGVTARAASRDQNLEVD
                     AVELLPEVIEAAHHFRSENDPAGKPRVFEADARRYIRSSRSTYDVIVSDNFHPARSGS
                     GALYTVEHFAAVRRVLNERGLFCQWLPLHQLDLDTLRSIVQSFVSVYPRSWALLANNS
                     LETPVLGLLTKADQTRLDLTLIR"
BASE COUNT         2075 a         3596 c         3253 g         2171 t
ORIGIN      
        1 ccttgcaact cgtgcagcca tctcccgcag tcgtgttgcc gtcatcgcaa gcctcaccgc
       61 tttgtatgaa gccgtctcca cacgccttgt ttttgcagcc actcgtgcag tcgtcggtgt
      121 cgaccatgtt gccgtcgtca cactcttccg gcgcctgctt cacgccatct ccgcagctca
      181 ccggcgcgca ctggccctgc acgcacttgc ccatgcaagc caggctctcc gtgctcacca
      241 gatccgggcc acacgtcagc gattgattgc tcgcgtcgca atatcggaag cccggcgtct
      301 gatcgacgca ctgagcaatg atctccttgc aggaaccgtc acggctatcg cagttctggc
      361 tagcggcaca gctctcgcca tcctcccagt atccgtcacg gcacacgcgg cgggtcttcc
      421 cggccgtccc gagacactcg taggttccgt cagcggcttt gcagggctct cccttcgggg
      481 gttcgggagg ggagccccct gtaccgcccg tgtccgtctc accgccatct gttccgcctt
      541 ccggattcgc ggtggtgcca ccagtcgggc tgccacccgt tccgccgttg gcgatgacgc
      601 ccccggcacc ggcattggtg cttccagctg agctgcttcc accgcgcccg ccggagccac
      661 cggttggacc gccaccggtt ccggctgtgc ggccgccgct cgttgagccg ctcgaaccgc
      721 tttcaccgcc ttggcccgcc ttgtaattgt gattcttgtc gacgctgcac gcgcacgcga
      781 gtgcgacaca cagatacttt agatgccaat tctgcccgta ccccatggtc tcggtctgcc
      841 tctttaatgc actggtgtca acgatccggg agagcgatgt gacacagaac ccgattggac
      901 gctcgcggta gcgccttccg cgtccagact ctggtgagcc acgccgccct gaactgcccg
      961 gtgcgggcgc gtccgccgca gcgaggccga aacacgaacc gcgggccggc ctagaccaag
     1021 tgcagattcc ggcggagcgc tcacccgctg aacacaacgc cgatgcgatc gatagcccaa
     1081 aacgcggcga cgccacccat ggcgtagacg gcgcggcgcg tccacgtttg cttgttcaga
     1141 ttcgaccgct ccacgaaccc ccccaacgcg agtatgaccc cgatcaccgc aagttgacca
     1201 agctccacgc ccaagttgaa gcaaagaagc gcggagggga cgtgcttctc cgggagcccg
     1261 atctccagca aagccgacgc gaaaccaagc ccgtgcaaga ggccgaaagc gaaggctact
     1321 agccatggtg cacggcgtgt gagcgagtcc gcggggcgca aacactcggc gcagacgagc
     1381 acgattgaaa gagcaatggt ggcttccaca ggtgcagtcg gaacgctcaa caagccgagc
     1441 gcggtgaacg ccaatgtgac actgtgggcg acagtgaagg ctgtaatcgt ggctaggagg
     1501 agccgcctac ctgttaccag aagagtgaga gcgatcacga acaacaggtg atcgaacccg
     1561 gtcaggatgt gctcgacacc cagccaggtg tagtccgcga tgataggttt cagcgaaatc
     1621 cagcctgttc gagcgccgcc gtagacggtc aggctgggag cgctcgccgt aacgacgcgg
     1681 agcaggcgcg cgccgtcttg ccattcgatc tcgaccatga agcgcatcga ggcgccttcc
     1741 agccatggaa aatcgatcct gccaaccata cccgaaggcc cgcagtcgat gtacgctccc
     1801 gctcgtacgc aaggtttcgg aaagatcgcc ggtgttacct gctgttccaa ggttggtgag
     1861 ccagacttga attgcatcga gaagtagccg tgacgcacct ccttcaggat caaagaggca
     1921 gagttgatgg ggtgagcgaa gctattttcc gccagcagaa gggaccacag ggcgagcaac
     1981 aaggtgctca gtcgcagccc gagcacgcga gtcacggctg ttcctcgaat ctgtagcgct
     2041 ccaccgtccg ttgcagaagg cgctctatcg cccgttcacg catcgccgcc ttccaatccg
     2101 ctagcactcc agggcgaatt tgttccaagc tcggcaatcc tcccccaagc tgcttaacct
     2161 gaacgagaaa cagagtatct ttggattcga ctcgcgccca agcccccagg ggaagcatgg
     2221 gtatccgctc tgccagttcc aggtcgaacc gctcccggat gtcagcaacc gacaagtttc
     2281 ccccgatcac gggacgccca agcatggctg gattcgttcc ttcgttgatc ttgcgcgcga
     2341 actgttcgag cttctccgga gcagtcggtt cggactttgg aaaggcgatg aactcgaagt
     2401 cgtatgttcg cggagattcg taatcgcttc tatgtgaatt gagccactcc gaaagctccg
     2461 cttcggaggg ctccggatct cgcacctcca actccgcgcg tgcgcgcatt ttcccgacca
     2521 gaacggctcg aacgtcggga tcatcctggt cgaggcgctc ccgcaaggac tcgcggaaga
     2581 gcgcttcatc ccgcttccag ttacgcactt ccgcttccag ctcactcgcc gtcggcttgc
     2641 gctcgtgcag gtcctcgaaa cgccgagcca tcgccgctcg gaccccagga gtaacgatga
     2701 tggtgcgcgg agtcccggct acccagcgct gcgcaagaaa tagaagcgct ccgatcaaga
     2761 agaagtgaac gacaggttca gtggctaacc ggcgccaaac cgggttccgg gacatctcgt
     2821 tcggcatcgc taaattcgtt cgcaccgtgc agggttccct tagcatcacc gcgaacacgc
     2881 tttaagagca gcgcgcggtc accaaggatt ttcaccaatg cgaggcccta aatgcaaaca
     2941 ccccccagcc gagcagacgc tagactgggg ggtcgttggc gcggaacgaa gccgcgcggc
     3001 gctcgatgaa tctccgctca gggtgacagc gtcaccgaga gcgcgtcgag cgagatctca
     3061 tagtacccgt gggggtccca aggcagggtg gtcccgttct gcttcaaagt accgccatcg
     3121 accttgaccc gcaggtagtt gcccttgggg aagttggccg gaagcgtcca ggtccaggtc
     3181 ttgtcggaac cggaggtcgt cgtcgtcgcg gccgcaaacg acttcgagcc gacccaataa
     3241 gaaccgatat tctcgaccgt atcgatccaa gcattgcccg agttcttcaa actcgtgaca
     3301 tggctggtga agctggagaa cgacgtcgct tggtaggctc cgtcggtagc cccctgatca
     3361 ttccagccgt gaatgcaaac gattatccac ttccctccgc tcactgcgct cgtaacggag
     3421 ccgtagtcag cgttgccgcc cgcgggcaac cacccgctca gattgtactg cgagctgatg
     3481 ggatcattga aacccgggtt cgttttgcca actccgcggt tgatgagata gcggttgttc
     3541 ttggccacat ctgcgtagcc atccgtgcca ttcggggctg ccatcgttac cggggtaacc
     3601 tggaacttcc cctggatgtc gctctgaccg ttcttgatat ccgggtctga cgcagtcccg
     3661 gaatgggtcc gagtgtgatt ggcaagctca tggcccgcag tcttggcttg gctccagact
     3721 gagtttccaa actcgttctg tttgccagtt tgcatgaaga acgtgaagcg cacgcctagc
     3781 gcgttcatct ctgcatatcg gttgatctgc gtctgattgg tgtcatcgaa cgtatacgac
     3841 accgcacctt tgaacccggc ccaaggcaga acggtaattg cgcccggggt cccgctcggc
     3901 ttcgccacac cgccattgcc cggtgcgggg tagcctgaag ctccaagcgg gcctgttcca
     3961 ccttgcggac cggagcctcc ggtcgaagtc gtgccgccca tgccggtcgc acccccggta
     4021 ttgccgccgc tgcctccgga gctgctgccg ccgctggctt tcccgccgcc gccaccactg
     4081 cccggaccgg cgccgccggt ggcgcttgtt ccgcccgccg aggtgccacc ggtgacggag
     4141 gttgagccac ccgcccctcc ggagttagag ccgccggtgg cgctcgcgcc gcccttcgcg
     4201 gaactgccgc cgctagtact ggttccgcct cctgacccgc cagaaacggc tcctccggtg
     4261 gcttgggtgg tgccacccgt ggtggtactg gtgccgccgg tttgcgcaga ggtgccgccg
     4321 gatgccttgg cgccgccggt gcccacgggt ggcgaaccac cagtgttgtt gttgatgggg
     4381 tcagtgctcg agcaactgac ggcaaaaacg atgacgccta gcgccgcaaa cgatcgatgc
     4441 aaagaaatca tcttggagtt atccgaactc gcaggtggct ttgcgacgtt cgtccttcgt
     4501 tgggagaaga attatccaac cagcaggaat atgctggttc cgaggtaggg accagggtgc
     4561 gcaagtctga gcggtacctg aggctcccgc ttctcatctc agacacaatc gtcgatcatt
     4621 tctgagcaac ccgcgtggct caccgattgt aggtccgttt tttcgagata cgtgtgccct
     4681 ccagagcacc attcccgcgc tcatccgagt gggtcagcgc ccaatcaccc cgagatgaag
     4741 ctcgatcgaa gccgcccatc aagttcgcgg gctgggatct acgtacgcga aacgtcggag
     4801 ggtgcgcacg ctgcaatgac gcgaacatgg caaggatgcg ccccagacga aaacttatgc
     4861 tgccgcacaa cttacgtcgt gctctcgtcc ttgtgtccga acgtatcgat tcacgcgata
     4921 gccccagagt gagctgcgcg tgattgcgac accctttagg tgtacggctc acctgatcga
     4981 agtcagccga gacaagctaa gggccagcga atgaatgaat cagtcgcgag ctggccttac
     5041 gaggcttcat cactccttgg gatttagtcg gagcaaagcg gtgggcaaga aagccttacg
     5101 acttctggca gccgaaggcg catcgttggt tcccccgcgc cccccttcaa cgcatcgtca
     5161 ccagctcctc ggcggcggtc gggtggatcg cgacggttcg atctagatcg gctttggtgg
     5221 cgcccatttt gagggccacc gcgaagcctt gaatgatctc gtcgcagttc ctgccgatca
     5281 ggtgaatacc gacgaccttt tcggtcgcac cgaccgtcac cagcttcatg acggtgggag
     5341 ttcgcctctc ggtcagacca tagtacatat cgaaaaatcg agttctgtag cacttcactc
     5401 catcttcgaa gcgctccaag gcctcttcct cactcaagcc aacgcttgcc accggaggat
     5461 ggctgaagat gacggtgggc acgtcggtgt agtcaagccg cgcgtccgcc ttgccaccat
     5521 agagtcgatc cgcgagcttg cgacccgcgg caatcgcgac tggtgtcagc tcgttcttcc
     5581 ccgtgacatc gccgagcgcg aatatgtcaa cggcgcgcgt agcctgccat tcgtcggtga
     5641 tcacgaaccc attctcgacc ggcacgccta cccgctcgag tcccaagtcc tgaattcgac
     5701 cacgacgccc gatggcccag atcacgtgct catacccacc ctccacgtga ccggtttcgg
     5761 aggtcagcgt gagcccctgc tcggttctct ccagtctctg cgcggtgaaa ccagtgatca
     5821 tgctcacccc cgccgccgtc atttgttcga tcagctcgtc cgtaatcgta cggtcgaagc
     5881 gccgcagcag acgttcgtgt cggatgatca gactaacgtc cacgcctaaa tgactgagcg
     5941 cacttgccag ctcgactgct atgtagcccg caccaacgat ggcgactctc cgcggtaagg
     6001 tttcccacgc gaagaagtca tccgaagtct ccccgagttc cgcgcctgga atattcggtc
     6061 gttccggagc tgacccggtg gcgatcagca agtgcttgcc ggtgacggtc tgctcactgc
     6121 cgtccagagc cgtgacgcgg atcgtgttgg gcccctcgat cttgccccaa ccccgaatca
     6181 gagtgacgcc gtcgacgccc agatttctct cgtaaatcgc gtttagccgc gccacatact
     6241 ggtcccgctt gttcttgaag cttcgataat cgagctgcgg gctgaactcc ccgagtgaat
     6301 agcctttggc gtcgtgaaga ctgtccaaga cgcgagatag gttccagacg atcttcttcg
     6361 ggacacagcc gcggttcaca catgtaccac cgagttcggc gccctcgatg atcgccgcac
     6421 tgacgccgta gctcgccgcg cgacgcgccg ctgccaaccc accgcttccc ccacctagca
     6481 ccaacaagtc ataaccgtgc ttggacatcg tcgcacagta cgagatctgc cggcggcggc
     6541 caagccaggc gcgcgtcagt cttgcgaggc caaagcgcag actaagcgtg acatccgaac
     6601 gatacccagg aagagcccgc ggtcatccgt acagatcagg gggtcccagc gcaccctctc
     6661 cggacgagtc gcggctcgtt gggccacgct ctccaaagcg ctatccggtt ttacacgcat
     6721 gagctcgtgc tcgctccgta cgtggtgctg ctctgcgttc aagaagcgga gcccgagtgg
     6781 ccttccctcg ccacttagcc gcacggctgt attctgagca ggccagtctt cgtccgacaa
     6841 gcaagtggcg cagggctcga gcaggggtcg caagatgcga gccgggcgta agctcggagc
     6901 ggcgttgacg agtactcgct gggcaaccgg tagaatgtcg gcccagggtg gcgccgggcg
     6961 cgccaagaaa tagccttgcg ccagcggaac cccgatctga cgcaagagag acagctcgct
     7021 ttcagtctcg acgccttcgg ccagaagcca agcatccagt ctcccggcga gctcacccag
     7081 catctggatc agcgcccgct tggcgtcgtc ttcatggaca ttcatcacca aggcgcggtc
     7141 gactttcaaa atctgcggtc gcaacgcaag gatctgctgc agcccggaat agcccgatcc
     7201 tgcgtcgtca acagcaacga gtgcaccgcg ctcgcgcagc cggccaagct ctgcggcgag
     7261 cgctttcata ttctcgattg ctcggtgctc cgtgagctcg agcacgatgc cacgcagatc
     7321 gggagtggcc tccaagacct tacgcaccgc cggttcgagc aagtgactcg gatccacgtt
     7381 catcgcgaag aagcagttcg ttggcttttg ctgggcgagc tcaaggcaac gcgctacgac
     7441 gacggactcc aactctgctc ccaatccctg acgcgccgcc tccgcgaaca cccggtcggg
     7501 ccccgcggga ggatcgagct ggaaacgtga cagcccctcg tagcccacca cctcaccacg
     7561 acgcagatcc acgatcggct gagcggcaag tgtcagccgg gtgcggtctt gtaggagcgc
     7621 ggcaatgtcc aaggcggaac gtgcccgagg cggaacgggg ttgactcagg cctggaccga
     7681 acggatgtgt tcgcgaacta ccttaatcaa ctcgggagga acatccttga tcccgtgtgc
     7741 gtcgcgagcg tgggccgcca cctctcgcag aacttcttca tcggaattgc aacggaattt
     7801 ggcggtgcac ccgggcacca cgtctccgca agaaaactct ttcattcgtc tcctccgtaa
     7861 acacgtacgg cttgctgcgg cactggcttt agttgagtag gccgaagaac gcaggtcagc
     7921 tcgcgcgaga atcgcggtgc ttttcgcccc agagctcgat tagtagaagc tttgatggtc
     7981 ggttttcgag ccccttgcgg cgttctgtac gcgggcagcg tactccgcga gaatgacaca
     8041 cggaagtccg gtgcggtgga cgccgtccgt cacgacccga tacgctgcag ccatgagatg
     8101 gcatctgctc ggttgctcct tggcgctcgt cgcatgcgcg gaaacaccac ctcctcccgc
     8161 gtcctccccc gacgcggtcg acaccgcgcc agcaagcggc cccccgagct caccccgtac
     8221 tgactctgct tcgtctccac ccgcaacgac tggagcgatg ccgataagtc ccagtggccc
     8281 atcagccccg ctcgttgtcc cgagcgtacc cgacgaaatc aaggttccgg gagacgcaac
     8341 actcatacag agaacgaacg cgcgcggagt ccagatctat acttgtgtcc cgaagcctgc
     8401 cgcaccgggg caattcgagt gggcgctcaa agccccggaa gcgtcgctga cggatgacgc
     8461 gggtaaagtc ctcggcaaac actatgcggg gccgacgtgg gaaagcacgg acggcagcaa
     8521 agtcgtgggt gcgatgaagg cgaaggtcga cagcccctcg cctacggcta ttccatggct
     8581 gctgattgaa gctcaatcca acgccggaga gggcgcgttt tctcggattg ccttcgttca
     8641 acgagtgaat acggagggcg gcaaagcgcc ggcccaaggc tgtgacaagg cccatgaaaa
     8701 gtccgaggct cgcgtcgatt acacggcgac gtattacttc tatgcacgct ccaagccata
     8761 accacttcgg cttagagaag actccacccc acagggattg gcatctagcc catcacgaga
     8821 gtcgtccccc cccctacacc cgagtcgctg aatgcgagct ccacgatatc gcgtcggtcg
     8881 gttgcgttgc gagtgcgtga cgatctcggg gcgcgacgtt tagtagtgga cttggagcag
     8941 gagctccagc catagaggcg ctgaatcggg atcagagaca tgtggatcac cgtgccgccg
     9001 ccgaacactc gggcttgccg caggcggtgc ggctcgcgat gaatgcccct atcgcatcga
     9061 ctagcggcgt tgcccgtacg ctatctcgcc tgcgtttgcc gctcctgatg tgcgtctcgg
     9121 gttttgcgag cctgacgtat cagatggttt ggacggctca gggtgcgctc tggattggtc
     9181 atgagtccgc cgcggttcta gccgtgatca ccgctttttt cggcggccta gcggtgggtg
     9241 ggctcgcctt cggaaagctg gcccagcata gcgccaatcc cactcgttgg tatggcgcgt
     9301 gtgaactgtg cattgccgcg tggggttgta ttctgttggc cgtaggagcg cacttccagg
     9361 ctgggctgca aaccttgctc gagtccccgg ctgagaacgg ctttccctgg ctcaccacct
     9421 ttctcctgat gttcctttgg ctgctgccag cgacgaccgc gatgggcgcc acacttcccg
     9481 ccatgcagcg cttgggttcc agacgggctg atggatggca cagcctgccc gagctctacg
     9541 cctgcaacac cttcggcgcc gtgctggggg tgctgctccc aacattctgg ctcatccccc
     9601 aattcggatt cacgagaacc ctgcaggtct gcatagcgct gaacctcgcc tgcgctgcga
     9661 gcccgctgtt cctcacgaat cgtgatccga ctcacggtag ctcaagtcac ctcgggatcg
     9721 gcgcaattac caagtccccg gaacccgagt cggcgtggcc ccactacatc acactagccg
     9781 tgacgggctt tttgggcatt gcgtacgagg tcctggtcgt acgggttctc agtcaagtca
     9841 ccgaagacac ggtctacaca ttcgcgctgc tgctttccgt atacctcgtt ggcacggcgg
     9901 tgggagcggg tgcttaccaa cgcttcgccg cgacaagcct tgtcactccg gcccggcttc
     9961 ggattcggct gctgggagct acggcaagcg cgtgcctcgt cggaactact tcactttggc
    10021 aatcgaactg gatccgagat acggcttcga gcgcgctggg ctcgagcttc cctgccgcca
    10081 ttgccaccga agcgctgctc gcgggctgtg cgttcggacc agccgcggtc gcgcaaggcg
    10141 ctctctttag ccacctcagc caaagtttga ttggggcggg cgcaaacttc ggcgtggcgc
    10201 tcggcatgaa cacactcgcg gggtcgctcg cgccaattct agcgggtgta gtcatggcga
    10261 ccgcattggg cagcaaggct tcactgctgt gggtcgcaat aggctatgtc ttgctagcgc
    10321 ggccatgggg ctcggcacga cgcgagctgg cgctctgggt accagcggct tgcgccatgg
    10381 gcttggccgt attcgcgccg ccattatcct tcctcgaggt gcctgagggc ggtcgcgtcg
    10441 ttagccacga ggagggggtc atggcggcgg tgagcgtggt cgaagatgcc cgcggcgtcg
    10501 cgcgactgag catcaacaat cgccaacaag aaggcagtag tgccagcgcc ctggtcgatg
    10561 cgcgccaagc ctggattccc ctgttgctcc atccgcaccc gcgtcgcgcg ctatttctcg
    10621 gcctcggcac tggcgtcacc gcccgcgccg cgagtcgcga tcagaatctc gaggtagacg
    10681 cggtggagct tctaccagag gtgatcgagg cggcgcacca ctttcgctcc gagaacgatc
    10741 cggcaggcaa gcctcgagtg ttcgaggccg acgcgcgacg ctacattcgc tcgagccgct
    10801 cgacttacga tgtcatcgtc tccgacaact ttcatccggc gaggagcggt tctggagcgc
    10861 tatacaccgt cgagcacttc gcggcggtgc gccgcgtttt gaacgagaga gggctgtttt
    10921 gtcagtggct accgcttcat cagctcgacc tcgacacgct gcgcagcatc gtgcaatctt
    10981 tcgtcagcgt ttaccccagg tcttgggctc tactggccaa taatagcctc gaaacgcctg
    11041 tcttgggctt gctgaccaaa gccgaccaga cgcgactcga tctcacgctg attcg
//
LOCUS       DASTKU010000662        15120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_349941, whole genome shotgun sequence.
ACCESSION   DASTKU010000662 DASTKU010000000
VERSION     DASTKU010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 15120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15120
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349941"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1647
                     /locus_tag="VFQ61_23030"
     CDS             151..1647
                     /locus_tag="VFQ61_23030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HET9957399.1"
                     /translation="MTSLSPKSERARAAFDEFLARDLDEVLTRCLRGEPARLALELFR
                     RTAASVPAYKAFLSDHGISPEQAETLDDLASVPVTTKENYHRRHSLDDLSREGRLSDC
                     DMLAVSSGSTGEPGVWPRFSSDELQTSVRFEQVLVDAMEVTNRRTLGVVCFALGTWVG
                     GMYTTACCRHLASKGYPLTLVTPGNQKAEILRVLRALAPAFDQIVLFGYPPFLKDVVD
                     SARGAGFDWSKHRVRLVMAGEVFSEAWRSSMCERLGAADPALTTASLYGTADGGVLAN
                     ETPLSIRIRRALAERPEAARALFGEARLPTLCQYDPCHRFFEVESDGSLLFTGDGGIP
                     LVRYKILDRGGIVEYRTMLAFLKQHGVALPELGTARELPFVFVFGRSNFAVSFYGANV
                     YPENVAAGVERPEFAEDLTGKFVLEVVEDTDQNPRLSVAVELAAGKQPDESLSTVLAA
                     TLREQLEHMNSEFLNYAPAERRTPLVRLLPQGHPEYFPLGVKHRYSRA"
     gene            2939..4867
                     /locus_tag="VFQ61_23035"
     CDS             2939..4867
                     /locus_tag="VFQ61_23035"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957400.1"
                     /translation="MDPNRLQSSKYPDGTDGAESEVSSPNQSRARALGNHLPGSGAPG
                     NGSPGSSIPGSSIPGSSVLESSVQNATLPSAGAGSGVLSEGIPSAGGFASGGLSSDAM
                     FSAGLPVAAGLSSGGVPSGTMPSAMPVAGSLSGPVPITDPNQAARMYGMLAPGSNFGV
                     YVVGNCIGEGGMARIYRAEHAGLRRHVALKVLTNGIAKDPDGRERFVREARIAAAIKH
                     PNVVNIFDVGVHDDIPYLVMELLEGTDLESLIKTKGALDESTILDIIVPVVAGLTAVH
                     DAGVVHRDLKPGNIFLSKGRYDEIEPKLLDFGISKAGEADKLRLTTHGLLVGTPFYMS
                     PEGLRGEEMTPHSDQYSLGVLMYECATGRVPFTASTFPDLFRVISNGDYPALTETNSK
                     VSKRLIRIIQRAMSLDPRQRFSDLRELGRELLLLSGQRTRITWGLSFGETRQHSQASR
                     LSSMVPVAGSLQPKPAPARRKLAPWLMLGAAAFLLMGFAVVKHTAWDTVAATPPTSAE
                     LPHSLPGTESATQVTQTPPSEVPNGLVDRADPASGSLVQGGSQVPASAVKAALGEKSE
                     ATGTGVLSGPEVLPLSVTAELPVKRVLTRPSMQQVRAPSRNNADTTRNSAGVSNSGSS
                     AKSAESMTTGSNGAPIFD"
     gene            4959..6566
                     /locus_tag="VFQ61_23040"
     CDS             4959..6566
                     /locus_tag="VFQ61_23040"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9957401.1"
                     /translation="MGPAELMAQQARLQGVLEPGSTFGVYIVRDCIGEGGMARIYRAE
                     HAGLRRQVALKVLINGFAREPEGRERFVREARIAAAIKHPNVVNIFDVGVYEDVPYLV
                     MELLEGTDLESFVQSKGPLDESVIIDIMVPIVAGLAAVHEAGIVHRDLKPGNIFLSRG
                     RYDEIEPKLLDFGISKAPDTEQAKLTMNGLLMGTPFYMSPEGLRGEDMTPLSDQYSLG
                     VVMYECVTGRAPFAPSTFPELANQITTGQYLLPTQHNPTVSKRLERIIVRAMSLDPKK
                     RFSDFREMGRELLLLAGQRTRITWGLSFGETSHTRTMAVVAPPPESIPNFRPNQWRKP
                     IAAIVAGLGIGLLANFGLPWSRSSEDRLGKLPASNAPTNPAQPPTSPRAVKPGADKPA
                     SEAGPSTDPALAANHADPRLTVRSAQTGPTQNADLAQGALAIQGAQAIQAAQASVGSQ
                     ARPHPAAASQQAGSTSLGRAKAGTNTTGADSRSSASADDAARSSAPAVRAPKSKSQST
                     GGSADSTKTKVSAAKTVSGANGAPIFD"
     gene            complement(6583..8244)
                     /locus_tag="VFQ61_23045"
     CDS             complement(6583..8244)
                     /locus_tag="VFQ61_23045"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HET9957402.1"
                     /translation="MSSSFTARPARAVDDPSNEHPPSRQLDPRHPGGAEQSESPARTE
                     SPDDKAVASPTDTPSGDVVESIEGDTATTSAPPPGMEGTSTPPNDELQKFELNGWARQ
                     TLELGFSNHARRDSQPEPGALPYDRFTSQSQLFVRARYARGRWFEAAVSGALTYSLFE
                     QGTDSSSKSFNGFNGQSARGVLEPRLQELFIGLFADKFDFRIGQQRLAWGRGEFVSPN
                     DVMNARDLRNPFVSETELRTLPTFLLRADLDLGFGSLQGVIAPVFTPDRFDVSDSNWA
                     ALQPDAPLWMRGLAGIARHSLDPTLQERAQRLLEATQYPKQNFTEPVLGARFAWSAGG
                     VDVNHYYQYGFDGPLVSVAPDLREALTNVDWATAGLTTLQPLFAAIDAGQNPIRVRYE
                     RRHHVGLDAATTVGPIAIRLDTAYQSKRGFFRRDFTSVVSPSLQTVLALEYQTFDKNK
                     LILIEGVYQRIMDTLPYPVLLYKLDSFAVAGTVRWPVWGAWGFDVRSIVGIYPRTLIL
                     QPELTFKPDPLNISLGGLWLDGAAFTLGDYYRRNREVYVKARYFF"
     gene            complement(8384..9253)
                     /locus_tag="VFQ61_23050"
     CDS             complement(8384..9253)
                     /locus_tag="VFQ61_23050"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HET9957403.1"
                     /translation="MLKQNLASIVLAALSVQLASAGVVARAAPGAAAKGPIKPAPNTV
                     AKAPSSPPAKASVQEILSGLIDADPWALGGAVMTARASLKDKHGASSELTFQARSRRY
                     APPFSKAIVRFTAPADLAGAGFLQVQNQTGDDDRFLFLPDLKRSRRISGNLRGSAFMG
                     TDFSFADLDRRDLRESSSLRKADESIGKFPCFRVDATPSRADSPYSRIEMWIRQDNYM
                     PLKMQMYDRASALFKTFTAQEVRRVSGHWYITKSRMIDHLNSHQTELVIENVAPSTDI
                     PDEEFTVRNLEKL"
     gene            complement(9265..11610)
                     /locus_tag="VFQ61_23055"
     CDS             complement(9265..11610)
                     /locus_tag="VFQ61_23055"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9957404.1"
                     /translation="MTRFIRFILKYRALVGALLVLSVVVSLYAARTIRARFQFRDFYD
                     YPGNTELPLFRQDIDQFGDPAGYVVAMVESDDVFRPDVVAYVQKLTQALEPERTFARV
                     RSLTNVHAIRGTADEVVSGPLYAQLVERPGGLAEFKRFALQSPLLRRRLVSADAKATA
                     VLAEMRTPTAFSTFDEHHAALGLVERAISRIPAPPGTEVRVTGAPAVEAGVTASLVSD
                     QLVLMPGVLVVLCIVLYLTFRSWHGIVLCLAAVGVATVWTAACFAQLHRPVDIIGSVI
                     PTTILVYGVVDPIFVLARVMLKLESGLKREEAIVRSFSELALPCFLTSLTTSIGFAAF
                     VTARQLTIRYYGLTVAIGVLLAWVTSMTVLPVLLSVVPLPKRQLSTGAWERGLDRALG
                     ALWDGLRKRVPHAIVASVVLLVAGSWFASKQHLDNVYVDELPHGKARRDVRHLEQKLS
                     GVLGLTVHLQGARDSMKRPAVLQAVQAVDTTMEREPAVTLSTSLSDLVAEANQAFLGG
                     DQQERRVPSSQALIAQYLALIEPSDRATFVSSDYSQSHLAIVITDQGSERTRALVAKL
                     RKAVESAGFVDLGVKAEITGTGVVGYTELDRVVEELLWGFITAFGLIVLLQWALFRSL
                     RIALIGIIPNLLPVVACFLTLRALGIHLRIDTALVLCISVGGLFNTTIHLAARVRQLA
                     IEGQRDADGIVGLAMRTVGPPSLFTAAALSAGFAVLLLSSFPGLQALGLLSMVTLVIG
                     FFADMIVTPVLLRAGFDWKSFLRAAPPSGGAVLPAIAPTVE"
     gene            11938..12972
                     /locus_tag="VFQ61_23060"
     CDS             11938..12972
                     /locus_tag="VFQ61_23060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957405.1"
                     /translation="MPRSLLLEAGPLRTSCDLTSADLEMPILNLVGRIPNQLALSALM
                     AVSWACSVGCTELSDPGDHRELPAEEVDPEWGCLKIPAVKPTYAEVPAYIIYTAPIID
                     FAARPPQEVSGLVVEFCPIGGQQGCTRIPFREPLSVTVKLNGAPVKVPVYALPVPYGA
                     IGYFRLTAPDYMPQEYYLGGPLIGAPGSVMVPFTLPDGTATTGAIMQAQPLTLVRTKD
                     ADDLAFKVMATRDPNQAIVAVRTVNCKYEPAAGVTITLERAANRPVPFTYRSSLALSS
                     DPDAPEPTDENGLAGFANIAFPTGNPGYSVLLEGTAPTGEKYGKIAILVRPGQISTAE
                     IRVSPEAFGR"
     gene            complement(13026..>15120)
                     /locus_tag="VFQ61_23065"
     CDS             complement(13026..>15120)
                     /locus_tag="VFQ61_23065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioester reductase domain-containing protein"
                     /protein_id="HET9957406.1"
                     /translation="IHAAGVLDDGLFAQQTPERFQAVMAPKVIGAWNLHLLTQSAGLD
                     WFVLVSSIAGTLGSSGQSAYAAANCYLDALAAARRARGLVASSIAFGPLSERGLAANL
                     DSRAQTRLSRQGFQFLTLQEASDQLLRSLQGGGENLAVAAIDLRKLSQSSRAQVPPLW
                     RALLRSPARSALRGAEVGPPSTFVKRLEALPREEQLALTTEIVRAEVARVLSLASPVA
                     VPLEKPLKELGLDSLMALELRNGLSRRTSITLPAAVLVQQPSVRSVSEYITASALGDA
                     GSAPGSSVVVKSAASVPFEMDATLDEEIRPPTATQAGAAPASDARSVLLTGATGFLGA
                     YLLAELLREPGTHIHCLVRARDAAAGRARVVENLRQYGLWNDAHGSRVHVLIGDLTQP
                     RLGLDASTFAELAEGVDAIYNNGAVLGFVADYPDLKASHVDATREVLRLASIGVAKFV
                     HHVSSAVVFDSSAYRGQELPESTLPVETRGLQLGYSQCKWVSENLIWKAAARGFPVTV
                     YRPAFIAGASTTGAWNTADFLCRTLKTTIELGTIPGDLELDLELSPVDYVSRAIVHLS
                     RQPDSRGRAFHLQHPSGLSFQRLAEILRSCGYGVREVPYWEWIAQIEARPTDPLAAVL
                     PFLAQRLPPDRLTHIERWQRQYRPKLTCMETLRTLGRANIECPAIDARLIGRYLDYLG
                     EIGFIPRKPAKAGAR"
BASE COUNT         2956 a         4740 c         4800 g         2624 t
ORIGIN      
        1 ccgacgcaga aggcccaagc ccttccggag gaggactgag tttcacgcct tggcggagca
       61 tgtgcccggg tatgtgaatc cagcgcatcg tcgatagttc cggcgcgcta ggtggacggg
      121 gtgcgactat gctcgggacc gaggttagaa atgacgtctc tgtcccccaa gtcggagcgt
      181 gcgcgcgctg ctttcgatga attccttgct cgagacctgg acgaggtgct cacccgctgt
      241 ttgcgcggag agcccgcacg cctagcgctc gagctgtttc ggcgtacggc agccagcgtg
      301 cccgcgtaca aagcattctt gtccgatcat ggaatttccc ccgaacaggc ggagaccttg
      361 gacgatcttg ccagcgtgcc cgtcaccacc aaggaaaact atcaccgccg ccacagcctg
      421 gacgacttgt cgcgggaagg gcgcttgagc gattgcgaca tgctcgcggt ctcctcgggc
      481 tcgaccgggg agcccggtgt ctggccacgg ttctcgagtg acgagctgca gacgagtgtg
      541 cggttcgaac aggtgctggt ggacgcgatg gaggtcacga accggcgtac attgggcgtg
      601 gtctgtttcg ccctcggaac gtgggtaggc ggcatgtaca cgacggcgtg ttgccggcat
      661 ttggctagca agggatatcc cctcacgctg gtaacccctg gcaatcaaaa agcagagatc
      721 ctgcgtgtgt tgcgggcgct cgcaccggcc ttcgatcaaa tcgtcttgtt tggttatcca
      781 ccatttctga aggacgtggt ggatagcgcg cgcggcgcgg gcttcgactg gagcaagcat
      841 cgcgtccgct tggtcatggc gggtgaggtg ttcagcgagg catggcgatc ttcgatgtgt
      901 gagcgtttgg gtgcggcgga tcccgcgctc acgacggcct ccttgtacgg aaccgccgat
      961 gggggagtac tggcgaacga gacgccattg agcattagaa tccgacgcgc cctcgcggag
     1021 cgacccgagg cggcgcgcgc gttgttcggc gaggcgcgcc tgccgacgtt gtgtcaatac
     1081 gacccttgtc accgcttctt cgaagtcgag agcgatggca gccttctatt caccggggat
     1141 gggggcattc ccttggttcg gtacaagatc ttggaccgcg gggggatcgt ggaatatcgc
     1201 acgatgctcg cgttcttgaa gcagcacggg gtagcgctgc ccgagctcgg aacagcccgt
     1261 gagttgccgt tcgtattcgt attcggtcgc tcgaacttcg ccgtctcttt ctacggggcc
     1321 aacgtttacc cggaaaatgt agcggcgggc gtggagcggc cggagtttgc cgaggatttg
     1381 accggtaagt tcgtgctcga agtcgtagag gacaccgatc aaaacccgcg gctctccgtc
     1441 gccgtcgaac tggcagccgg gaagcaaccc gatgagtcgt tgagtacggt cttggccgcg
     1501 acgcttcggg aacagctgga acatatgaat agtgaatttc tgaactacgc ccccgccgag
     1561 cgccgtactc cgctcgtccg gctacttcct cagggccatc ccgaatattt cccgctgggc
     1621 gtcaaacatc gctactcccg ggcctgaagt ttgttcgcgc cccagaattt cagggcaact
     1681 ttcccatgaa gggaaaaacc caaatggaaa cgggccccgt cggaaataca tgcctgacca
     1741 tcgtgcggac ctgaccaatc tagagcgccg ttctcgcaaa ttacctcgat gcgctccgtt
     1801 tcggccctat tcgaacatcg tccaactgaa accctggata ccaccaagcc tggatgcggc
     1861 ggcccaggat acgaaggccc aggatacgaa ggcccaggat acgtaagcga aacaggtcca
     1921 agccggcatt ctcatcggga gagcggcgtg gccgggttta tcggtcgctg cgctagttag
     1981 aacctatccc agtagttggg ataggttcta actaggtaaa agcacggttc gtgattgtgg
     2041 tttcccaaac ggggaggcct gggccaagcc aggctgagtc gtgtcgaggg gccggtgcgc
     2101 gaccgccaag ccagcactct cagcagctac ttagctactt ggcttcgcag cttccgcaga
     2161 ggcagagcag aggcacagca gaggcacagc agaggcacag cagaagcaga acagctgagc
     2221 cagactgata ttgaaccgag gcctgaccag aagagccaaa ggctcgccgg atgccggaac
     2281 gcccgcggag agtgtcggcg ggagtagtga ttcgcgattc gcgacaacac cgatcctgcc
     2341 ccgcaattgt cgctgaaaga ctgtccgttc ccgccaagct ctggcgtgcc ccgctaggga
     2401 tctccgtagg acaaaggggc catttctagg cgcaaagcgc catagccgcc gagaaatgcg
     2461 cgcctctgcc gcgaaaatac ctcgccgagg ctcccccgcg tgctgagctc ccgagaaccg
     2521 gcactccaaa gcacgctcac gcgaatgaac acgcccaagg cttgagtgcg tgttggcgcc
     2581 cagaaaaccc gcgcaagcag tcttgtcacg acgtattccg aaccggaacc tctcctgcga
     2641 gtcagattgc attcgatagc gaacgcccga ccgactgcgg tcaaaagaac tcgctgcgaa
     2701 tagagctgca gacgccaaac agcccgccaa aaggctgcta tagaggaggc ttacgagatc
     2761 tgattctcgg tggttgattt gagagcgcat ctgtctaggc ggggtacgcc caggacccga
     2821 gcccggaagc agacgcgaca gtttggaacg cggtagtcgg cagcagacag ttggtagcag
     2881 tggcgaagcg gtggagcgca agggctcccc gcgtggcaag cagaaaacgg acggcgctat
     2941 ggatcccaat cggcttcagt cttcgaagta cccggacgga acggacggcg ccgaatccga
     3001 ggtttcgtcg cccaaccaga gtcgcgcacg cgcgctcgga aaccatctgc ccggaagcgg
     3061 cgctcccgga aacggtagcc ccggcagcag tatccctggc agcagtatcc ccggcagcag
     3121 tgtgctggag agcagtgtgc aaaacgcgac actcccttcg gctggcgccg gaagcggagt
     3181 tctgtcggag ggcattccgt cagcaggggg attcgcgtcg ggcgggctgt cttcggatgc
     3241 gatgttttcg gcggggttgc ccgtggccgc aggcctgagc tccggtggag ttccttctgg
     3301 gacgatgcct tcggcgatgc cagtcgcggg cagcttgagc ggcccggttc cgatcacgga
     3361 ccccaatcag gcagcgcgaa tgtacggcat gctcgcgccg ggctctaact tcggggttta
     3421 tgtcgtcggc aactgcatcg gcgaaggcgg gatggcccgc atctaccgcg ccgagcacgc
     3481 cgggctgcgt cgtcacgtcg cgttgaaggt gctcaccaac ggcatcgcca aggaccctga
     3541 tgggcgcgag cgattcgtgc gggaagcgcg gattgccgcc gcgatcaagc accccaacgt
     3601 cgtcaacatc ttcgacgtcg gggtgcacga cgacatcccg tatttggtca tggagctgct
     3661 cgaaggcacc gacctcgaga gcctcatcaa gaccaagggg gccctggacg aaagcacgat
     3721 tctggacatc atcgttccgg tcgtcgcggg cctcaccgcg gtgcacgacg cgggcgtggt
     3781 gcaccgcgat ctgaagcccg gcaacatctt tttgtccaaa ggcagatacg acgaaatcga
     3841 gcctaaactg ctcgatttcg gcatctccaa ggcgggcgaa gcggacaagt tgcggctcac
     3901 cacgcacggt ctgctggtgg gaaccccgtt ctacatgtct ccggagggtc tgcgcggcga
     3961 ggagatgacg ccgcactccg atcagtactc gctcggcgta ctgatgtacg aatgtgccac
     4021 tgggcgggtg ccgttcaccg cgtccacgtt tccagacctg ttccgcgtga tctccaatgg
     4081 ggattacccg gccctcaccg agaccaactc caaggtctcg aagcgcttga tccgcatcat
     4141 ccagcgcgcc atgagcctgg atccgcggca acgcttctcg gatctacgcg agctcggacg
     4201 ggagctcttg ctgctgtcgg ggcaacgcac gcgcatcacc tggggcttga gtttcgggga
     4261 aacacgccag cactcgcagg cgagccggct gagctcgatg gttcccgtcg caggctctct
     4321 ccagccgaag cctgccccgg cgcggcgcaa gttggcccct tggttgatgc tgggcgcagc
     4381 ggcatttctg ttgatgggtt ttgcggtggt gaagcacacc gcttgggaca cggtggcggc
     4441 tactccgccc acgtcagccg agctgccgca ctctttgcct ggcaccgagt cggcgaccca
     4501 ggttacgcag acgccgccgt ccgaagttcc gaacgggctc gtggatagag cggatcccgc
     4561 gagcggttcg ctggtacaag gcggttcaca agttccagcc agcgcagtga aggctgcgct
     4621 gggggaaaag agcgaagcca cgggcacggg tgtgttgtcg gggccagaag tcttaccctt
     4681 gagcgtgacg gcggagctgc cagtcaagcg cgtgctaacg cgacccagca tgcagcaagt
     4741 gcgtgcgccc agtcgcaaca acgcggacac cacgcgcaac agcgccggcg tgtctaattc
     4801 cggcagctcg gcgaaatcgg cagagtcgat gacgacagga tcgaacggtg cacctatctt
     4861 cgattgatac agctcagttg agtgttcatc acgtgcgcga aatgactatc gtgtcagggc
     4921 tcgaccttcg ctaactgacg cttgatgctt ggtgcgtgat gggaccggcg gagttgatgg
     4981 cgcaacaagc ccgtttgcag ggcgtgctcg aacctggttc gacgttcggc gtctacatcg
     5041 ttagagattg tatcggtgaa ggcggcatgg cgcggatcta tcgcgcggag catgcgggcc
     5101 tacgccgaca ggtggcgctg aaggtcttga tcaacgggtt cgcgcgcgag cccgaagggc
     5161 gcgaacgctt cgtgcgcgag gcgcggatcg cagcggcgat caagcacccc aacgtggtca
     5221 acatcttcga cgttggcgta tacgaagatg tgccttacct cgtgatggag ctactcgagg
     5281 gcaccgacct cgagagcttc gtgcaatcga aggggccgct cgatgagagc gtgattatcg
     5341 atatcatggt gccgatcgtg gcgggcctcg ccgcggttca cgaagccggg atcgtgcatc
     5401 gcgatctgaa gcccggaaac atcttcctat cgcgtggtcg ctacgacgag atcgaaccga
     5461 agctgctcga tttcgggatt tccaaggcac ccgacacgga gcaagccaag ctcaccatga
     5521 atgggctcct gatggggacg ccgttctata tgtcgcccga aggtctgcgc ggagaggata
     5581 tgacgccgct ctccgatcag tactcactcg gagtggtgat gtacgagtgt gtgaccggac
     5641 gcgcgccgtt tgcaccgagc acgtttcccg aactagccaa tcagatcacc accgggcaat
     5701 acctgctgcc cacccaacac aacccgaccg tctcgaagcg cctcgaacgg atcatcgtgc
     5761 gcgccatgag cctggatccg aagaagcgct tctcggattt ccgtgagatg gggcgcgagc
     5821 tcctactgct cgcggggcag cgtacgcgca tcacgtgggg cctcagtttc ggcgagacga
     5881 gtcacactcg gaccatggcc gtggtggcgc cgccgcccga gtcgatcccc aacttcagac
     5941 cgaaccaatg gcgtaagccg atcgccgcga tagtggcggg gttgggaatc ggtctactcg
     6001 cgaatttcgg gcttccttgg tcgcgctctt ccgaagaccg gctcggaaaa cttcccgctt
     6061 cgaacgcacc aacgaacccc gcccagccgc caacctcacc gcgggcggtg aagccaggag
     6121 cggataaacc cgcatccgaa gctggaccgt ccacggatcc ggccctggcc gcgaatcacg
     6181 ctgacccgcg cttgacggtt cgctccgcgc agacggggcc gacccaaaac gctgacctgg
     6241 ctcagggggc tctcgctatc cagggcgcgc aagcaattca agccgctcag gcaagcgtag
     6301 gttctcaggc caggccgcat ccagcggcag cctcgcagca ggccggctcc acgtcattag
     6361 ggcgcgccaa agccgggacg aacacgacgg gcgcggattc ccgctcttcg gcgtcggctg
     6421 atgacgcggc acgttcttcg gcgccggcag tgcgcgcccc gaaatcgaaa tcgcagagca
     6481 cgggcggcag cgcggactcg acgaagacca aagtgagcgc cgccaaaacg gtatcgggcg
     6541 cgaacggcgc gcccatcttc gattgatcgc gacgaccgtc gctcagaaga agtatcgggc
     6601 tttgacgtac acctcgcggt tacgccgata gtaatccccg agcgtgaagg cggcgccgtc
     6661 gagccataac ccccccaggc tgatgttcaa gggatcgggt ttgaacgtca gctcgggttg
     6721 tagaatcagc gttcgtggat aaatcccgac gatgcttcgg acatcgaagc cccaggcacc
     6781 ccacaccggc cagcgcacgg tacccgctac cgcgaaggaa tccaacttgt agagcagcac
     6841 cggatacggc agcgtatcca tgatgcgctg ataaacccct tcgatcagaa tcaacttgtt
     6901 cttgtcgaag gtctgatact cgagggcgag caccgtttgc aggctcgggc tgacgacgct
     6961 cgtgaagtcc cggcgaaaga acccacgctt gctttgatat gccgtatcca ggcggatcgc
     7021 gatggggccg accgtggtcg ccgcgtccaa acccacgtgg tgacgtcgct cgtagcgaac
     7081 tcggatcgga ttttgtccgg cgtcgatggc cgcgaacagc ggctgcaacg tggtcaggcc
     7141 ggccgtcgcc cagtcgacgt tggtgagtgc ctcgcgcaga tcgggagcaa cgctcacgag
     7201 cgggccatcg aagccgtact ggtagtagtg attgacgtcc accccgcccg cgctccacgc
     7261 aaatcgagcg ccgagcactg gctccgtaaa gttctgcttc gggtactgag tcgcttcgag
     7321 caagcgctga gcccgttcct gcagcgtggg atccaaggaa tggcgtgcga tgcccgccag
     7381 accgcgcatc cagagcggcg cgtccggctg gagcgcggcc cagttcgagt ccgaaacatc
     7441 gaagcgatcc ggcgtgaata ccggcgcgat caccccctgc aggctaccga aacccaaatc
     7501 cagatcggca cgtagcaaga acgtcggcaa cgtccgcaat tccgtctcgg agacgaacgg
     7561 atttcgcagg tcgcgcgcgt tcatcacgtc gttcgggctc acgaactcgc cgcgccccca
     7621 agccaagcgc tgctgcccga ttcggaaatc gaacttgtcc gcgaacagcc cgatgaagag
     7681 ctcctgcagg cgcggttcga gcacccctcg cgcgctctga ccattgaagc cgttgaagga
     7741 cttggatgaa gaatcggtgc cttgctcgaa caggctgtac gtcaacgctc cggagaccgc
     7801 cgcctcgaac caacgccctc gcgcgtagcg cgcgcgcacg aacaattggc tttgcgacgt
     7861 gaaccgatcg tacggcaacg cgccgggctc cggttgcgaa tcccggcgtg cgtggttcga
     7921 gaagccgagc tccagcgtct gacgtgccca gccgttcaac tcgaacttct gcagctcatc
     7981 gttgggcgga gtgctcgtcc cctccatccc aggcgggggc gcactggtgg tggccgtgtc
     8041 cccttcgatg ctctcgacga cgtccccgct gggcgtgtcg gttgggctgg ctaccgcctt
     8101 gtcgtcgggc gactccgtgc gagcgggaga ctcactctgc tcggccccgc cggggtgtct
     8161 cggatctaat tgcctcgaag gggggtgctc gttcgaagga tcgtctacgg cgcgcgccgg
     8221 gcgcgccgtg aatgagctgc tcactgcaat ccaagctgcg aggcgaagcc ccaaacgaag
     8281 gcacatgcgg gcggttcttc gaagttcgag ttcgggccga agccttggag cgcgaagcca
     8341 cacggctcgt gggctacggg tcttcagcca gcaggttgag ggttcagagc ttctcgagat
     8401 tgcggacggt aaactcctcg tccgggatat ccgtagaggg cgccacgttt tctatcacga
     8461 gttccgtttg gtgagaattc aggtgatcga tcatgcgcga tttcgtgatg taccagtgac
     8521 cgctcacgcg ccgaacctct tgggcggtaa acgtcttgaa caaggcgctc gcgcgatcgt
     8581 acatctgcat cttcaggggc atgtaattgt cctgccggat ccacatctcg atgcgtgagt
     8641 acggggaatc tgcgcggctc ggcgtggcgt ctacccgaaa gcatggaaac ttaccgatac
     8701 tttcgtcggc ttttcgcaag gagctgcttt cgcgcaagtc ccggcggtcc aaatccgcga
     8761 aagagaagtc cgtgcccatg aatgcgctgc cacgcaggtt tccggagatg cgccgcgagc
     8821 gcttcaggtc gggtaagaac aggaaccggt cgtcatcacc agtttggttc tgtacctgca
     8881 ggaatcccgc gccggccagg tccgccggcg ccgtgaagcg cacgatggcc ttggagaagg
     8941 gcggagcata gcgtcgcgaa cgagcttgaa acgtgagctc gctgctcgcc ccatgcttgt
     9001 ctttcagaga ggcccgcgcc gtcatcaccg cgcctccgag ggcccacgga tcggcgtcaa
     9061 tcaggccgga gaggatctcc tggacgctgg ccttggccgg gggactcgaa ggtgctttcg
     9121 cgaccgtgtt gggcgccggc ttgatgggcc ccttcgcggc ggcgcccggc gcggcgcgag
     9181 ccaccacgcc ggccgacgcg agctgcacgc tgagcgctgc caaaacgata ctggcgaggt
     9241 tttgtttgag catcacgtct tctcctactc gaccgtcggc gcgatcgccg gaagcactgc
     9301 cccgccggaa ggcggagccg cgcgcaagaa ggacttccaa tcgaagccgg ccctgagcag
     9361 caccggagtc acgatcatgt ccgcgaagaa accaatcacg agggtgacca tcgaaagcaa
     9421 cccaagcgct tggagcccgg gaaagctcga gagcagcagg accgcgaagc cagcggacag
     9481 cgctgccgcg gtaaacaagg acggagggcc aacggtgcgc atggccagtc ctacgatccc
     9541 gtccgcgtct ctctgccctt cgatcgcgag ctggcgcacg cgagccgcga gatggatggt
     9601 cgtattgaac aagcccccca ccgagatgca aagcacgaga gccgtgtcga ttcgcaagtg
     9661 aatgcccaag gctcgcaagg tgaggaagca cgcaaccacc ggcagcaagt tgggaatgat
     9721 gccgatcagc gcgatgcgca gggatcggaa aagtgcccac tgcagcagca cgatcaaacc
     9781 gaaggcagtg atgaagcccc acagcagctc ctccacgacg cggtcgagtt cggtgtagcc
     9841 cactacgccc gtgcccgtga tctcggcttt cactcccaga tccacgaaac ccgcgctctc
     9901 cacggctttg cgcagcttgg cgaccaacgc ccgcgtcctc tcgctacctt gatcggtgat
     9961 cacgattgcc aaatgagact gtgaatagtc gctggacacg aacgtggccc gatcgctcgg
    10021 ctcgatcagc gcgagatact gcgcgatcag ggcttgtgag ctaggcacgc ggcgttcctg
    10081 ttggtctccg cccaggaagg cctggttggc ctccgcgacc aaatcactga gcgacgtcga
    10141 gagcgtgacc gccggctcac gctccatggt cgtgtccacg gcttggacgg cctggagcac
    10201 ggcggggcgt ttcatgctgt cgcgcgcgcc ctgcaagtgc acggtgaggc cgagcacgcc
    10261 ggacaacttt tgctcgaggt gtcgcacgtc acggcgcgct tttccgtgcg gcaactcgtc
    10321 cacgtagacg ttgtccaggt gctgtttact cgcgaaccaa gagcccgcca cgaggagcac
    10381 caccgaagca acgatcgcgt gcggaacgcg cttgcgcagc ccatcccaaa gtgcgcccag
    10441 cgcccgatcc aagccgcgct cccacgcgcc cgtcgaaagc tgacgtttgg gcagcggcac
    10501 gaccgagagc aacacgggta acaccgtcat cgatgtcacc cacgccaaca gaacgccgat
    10561 cgcgacggtc agcccgtagt aacgaatcgt tagttgtcga gccgtaacga aggccgcgaa
    10621 cccgatcgag gtcgtcagcg aggtgaggaa acaaggcaat gctagctcgg agaaggagcg
    10681 cacgatggct tcttctcgct tcagccccga ctcgagcttg agcatcacgc gtgcgagcac
    10741 gaagattggg tcgacgacgc cgtaaacgag gatcgtggtc ggaatcaccg atccaatgat
    10801 gtcgacgggg cgatgcagtt gagcgaagca tgccgccgtc cagacggtcg cgaccccgac
    10861 cgcggccaga caaagcacga tgccgtgcca cgatctgaag gtcagatata gcacgatgca
    10921 cagcaccacg agcacgcctg gcatcagcac cagctgatca ctcaccaagg atgcggtgac
    10981 tcccgcctcc accgccgggg caccggtcac acgaacttcg gttcccgggg gagcagggat
    11041 gcgtgaaatc gcgcgctcga ccaagcccaa ggcggcatgg tgctcgtcga acgtggaaaa
    11101 cgcagtgggt gttcgcatct ccgcgagcac ggccgtagcc ttcgcgtccg ccgaaacgag
    11161 ccgacgtcgg agcagcgggc tctgcagagc gaagcgcttg aactccgcaa gcccacccgg
    11221 cctttccacg agctgcgcgt aaagcggccc gctcacgacc tcgtcggcgg tgcctcgaat
    11281 ggcgtgcacg ttggtcaggc tgcgcacccg ggcgaaggtc cgctcgggtt ccaaggcttg
    11341 cgtgagcttc tgaacgtacg ccacgacatc gggtcgaaag acgtcatcgc tttcgaccat
    11401 cgccacgacg taacccgccg gatctccgaa ctggtcgatg tcctgtctaa acagcggaag
    11461 ctcggtgttt cctgggtagt cgtagaaatc acgaaactgg aaacgcgctc gaatcgtgcg
    11521 cgccgcgtac aacgagacga ccaccgagag caccaacagc gcccccacca gggcccggta
    11581 cttgagaatg aatcgtatga agcgagtcac ccggccatct ctccgccgag aacctatcgc
    11641 agggagctct tccaggcgag ggtcgcgtcc aaaggtttat ggggtttact gggagaaagc
    11701 tctatctcag cttcgcggtc ggaggctgct gtgcggagcc gcggctacgc acgaatcata
    11761 cgcaccggaa ctgaatgggc ttcccgtgct gccggttggc cgcatcgcgt atccaggccc
    11821 gagaatgccc aacaccggcg gggctggtga gctcggggcc ggttgacggc cgccggggtt
    11881 cccggtattg ccaaggcttg ctcggggtga cgccaagatg agaaagtcca ggaaagcgtg
    11941 cctcgttcgt tactcctcga ggccggccca ctccgaacct cctgtgacct gacctccgct
    12001 gacttagaga tgcctatctt gaacttggtt gggcgcatcc cgaatcaact ggctctcagc
    12061 gcgctcatgg ctgtgtcttg ggcctgctcc gttggctgta cggaactgag tgaccccggt
    12121 gaccaccgtg agctgcctgc ggaagaggtc gacccggagt ggggctgttt gaagattccg
    12181 gcggtcaagc ccacgtacgc agaagtcccg gcctacatca tctacacggc ccccatcatc
    12241 gatttcgcgg cccgaccgcc acaagaggtg agcggcctcg tggtggagtt ctgtccaatc
    12301 ggcgggcaac agggctgcac caggatcccg tttcgtgagc cgctcagcgt cacggtcaag
    12361 ctcaatggcg cccccgtcaa agtacccgta tacgcactgc cggtaccgta cggtgcaatt
    12421 ggttacttcc ggctgaccgc gccggactat atgccccaag agtattactt gggcgggccg
    12481 ctgattggag cacccggcag cgtgatggtt ccgttcacgc taccggacgg aacggccacg
    12541 acgggcgcga tcatgcaggc gcaacccctc acgttggtcc gtaccaaaga cgcggatgac
    12601 ttggcgttca aagtgatggc tacgcgcgac ccaaaccagg cgatcgtcgc ggtgcgcacc
    12661 gtcaactgca agtacgagcc cgcggccggg gtgacgatta cgttggagcg agccgccaat
    12721 cgcccagtgc cgttcaccta tcgcagcagc ctggcgctct cgagtgatcc ggatgcaccg
    12781 gaaccgaccg acgagaacgg cttggccggc ttcgcaaaca tcgcattccc cacgggcaac
    12841 ccaggatact cggtcttgct cgaaggcacc gcacccaccg gtgagaagta cggaaagatc
    12901 gcgattttgg tacgacccgg gcagatcagc acggccgaaa ttcgcgtaag tccagaagcg
    12961 ttcgggcgct gagcggaggc gccccggggc tcagccgggc gtcttctacg aggccgcgaa
    13021 tcggttcaac gagccccggc cttcgccggt ttgcgcggga tgaacccgat ctcgccgagg
    13081 tagtcgagat agcggccgat caagcgggcg tctatggccg ggcactcgat gttcgctcgc
    13141 ccgagtgtgc gcagcgtctc catgcacgtg agttttggcc tgtattgccg ctgccaacgc
    13201 tcgatatggg tgaggcgatc gggtggtaag cgctgcgcca ggaacggcaa cacggccgcc
    13261 aacggatcgg ttggacgcgc ttcgatttgc gcgatccact cccaatacgg aacttcccgg
    13321 accccgtacc cgcacgagcg caagatctcc gcgagccgct ggaagctcaa ccccgacggg
    13381 tgttgaaggt gaaaggcgcg cccgcgcgag tccggctggc gcgagaggtg aacgatggcc
    13441 cggctgacgt aatctaccgg agacagctcg agatcgagct ccagatctcc ggggatcgtt
    13501 ccgagctcga tggtcgtctt cagcgtgcgg cacagaaaat ctgctgtgtt ccacgcgccg
    13561 gtcgtgctcg cgccggcgat gaaggcgggg cggtatacgg tgacgggaaa gccgcgcgcg
    13621 gcggccttcc agattagatt ctcgctaacc catttgcact gcgagtagcc gagctgcaac
    13681 ccccgggtct cgacgggaag cgtggactcc ggcagctcct gcccgcgata agcactcgaa
    13741 tcgaacacca cggcggagga gacgtgatgc acgaacttgg caaccccgat acttgcaagc
    13801 cgcaagactt cgcgagtggc gtccacgtgc gaagctttca aatcgggata gtcggcgacg
    13861 aagccgagca cggcgccgtt gttgtaaatg gcgtccacgc cctcggcgag ttcggcgaac
    13921 gtgctggcgt cgagcccgag gcgcggctgg gtcaagtcgc cgatcagcac gtggacgcgc
    13981 gagccgtgcg cgtcgttcca caacccgtat tgccgtaagt tctcgacgac gcgggcacgc
    14041 cccgccgccg cgtctctcgc gcgcaccaaa caatggatgt gagtaccggg ttcgcggagc
    14101 aactcggcta gcaaataggc ccccaaaaag cccgtggccc ccgtgagcaa caccgagcgg
    14161 gcgtcgctcg cgggagccgc gcccgcctga gtggctgtgg gagggcgaat ctcctcgtcg
    14221 agcgtcgcgt ccatttcgaa tggcacgctg gccgcggact tcacgaccac gctcgagccg
    14281 ggagcgctcc ccgcgtctcc cagcgcgctg gccgtgatgt actcggagac gctgcgcacg
    14341 gaaggttgct gtaccaacac cgccgcgggc aaggtgatgg aagtgcggcg cgacaagcca
    14401 ttccgcagct cgagcgccat cagcgagtcc agccccaact ctttgagtgg cttctccagt
    14461 ggcaccgcca ccgggctcgc gagcgagagc acgcgcgcca cttcggcccg cacgatctcc
    14521 gtagtgagcg ccagctgttc ctctcgtggc aaggcttcca atcgcttgac gaaggtactg
    14581 ggtggcccaa cttccgcgcc gcgcagcgcc gagcgcgccg ggctcctcag cagcgcgcgc
    14641 cagagcggcg gaacttgcgc cctcgaggac tggctcagct tacgcaagtc gatggccgca
    14701 accgccaaat tttcgccgcc accttggagt gagcgcagca gctggtcgga cgcttcctgc
    14761 aaagtgagga actgaaaacc ttggcgggag aggcgcgtct gggcgcgaga atccaggttc
    14821 gcggccaacc cgcgctccga caaaggcccg aaggcgatgc tcgaagccac gagccctcgc
    14881 gctcgacgcg cggcggcgag cgcgtccaag tagcaattgg cggcggcgta ggcgctctgt
    14941 cccgaggacc ccaacgttcc cgcaatcgaa gaaacgagga cgaaccaatc caacccagcc
    15001 gattgagtga gcagatgcag attccaggcg ccgatcacct tcggagccat caccgcttgg
    15061 aagcgctcgg gcgtttgctg agcgaacaag ccgtcgtcga gcaccccggc cgcgtggatc
//
LOCUS       DASTKU010000663        13697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1243412, whole genome shotgun sequence.
ACCESSION   DASTKU010000663 DASTKU010000000
VERSION     DASTKU010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13697
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243412"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFQ61_23070"
     CDS             <1..279
                     /locus_tag="VFQ61_23070"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HET9957407.1"
                     /translation="GWLFFLTCSVVLAFAAFWELLEWWVTLLVASDTGQAFLGSQGDI
                     WDAQWDMLLAGVGAVVALTAFGRFHDRSMARVPFPHRVQASTPPARLA"
     gene            417..2240
                     /locus_tag="VFQ61_23075"
     CDS             417..2240
                     /locus_tag="VFQ61_23075"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9957408.1"
                     /translation="MRYPRGDDRTPRHFSESGLRVQNSLDRAEREEQKPPGSGEFVRA
                     RLELKLDALQLERRYRDSLKLIELNRALVGAHDFEQVAALVCSAVRALTGADGATFVL
                     REGDQVHYAAEDAISPLWKGQSFPIERCISGWAILHRAVAVIEDIFSDPRIPVEAYRP
                     TFVRSLVMTPVGPGAPVASIGAYWSRRYRASGYSLELLDAIASAADLALSSVRAYEES
                     ERARLEAARANELKDQFLATLSHELRNPLSSMVAYSELLLRSSDTRQIPLVRTAADSI
                     HRNAKAQAQIIHDLLDFSRIQTGKFALERQLISLAPLVGEAVESVRARAQEKRIKLDV
                     DLPAEPFVCHADPVRIQQIVWNLVSNAVKFTAQGGRVCVHLTREGAQAQLVVQDNGQG
                     IAPEFLPHVFEMFRQGDEHKPGPQGGLGIGLALVKQLAELHGGSVEASSEGLGCGARF
                     VVRLALQPDTAPHHSSSAGGRSPSHAAGPSLPGARILVVDDTEEFLEVIALLLGNEGA
                     QVTTARSAEEALKTAQSHHFDLVVSDISMPDVDGYQLVQALRLLPSFASTPTIALTGF
                     GRQEDVERTRCAGFSAHLTKPVHIGHLVELARVLIPPRAPQ"
     gene            2375..3613
                     /locus_tag="VFQ61_23080"
     CDS             2375..3613
                     /locus_tag="VFQ61_23080"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9957409.1"
                     /translation="MERWIESLQRRAATQEDGLPLSGGLPLAELFPQKELLACHAALS
                     LEADSSALQYTWPEGSEALRTWIAARLRKRGANTTPADVIITTGAQQALVMLCAVLKL
                     EGRRIGVDAATYSGALDVFRRARALPVVSTRAAAIYAIPGSSNPEGRGLGAPRRAELL
                     EAGCPIIADEAYAELRYDGVLERPLIADAPDRVFHVGTLSKTLCPGLRLGWLVAPSQL
                     RSPLLDAKRDLDLQAPSLSQALVERLLNDFDWDAHLEVQRREYRARMATLMGSLRRHL
                     PELEFEEPEGGFTLFVRYAGSPGQSPSGLDEPLLLDCATRHGTSFDPGSMFQAEPAPL
                     ALRLSPCGVGKEQIDSAVQRLAAAFSEARDRVATKTAQTSGAAPSAPSTGGQELDDSP
                     QLHDSPPGATRSERSTGTDG"
     gene            complement(3509..4696)
                     /locus_tag="VFQ61_23085"
     CDS             complement(3509..4696)
                     /locus_tag="VFQ61_23085"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HET9957410.1"
                     /translation="MHDSTEFQPRWVCPGCRRPRVTCYCSELRTIQTRTRVLLLQHPR
                     ERDMPIGTARMAHLSLPNSELLVGFDWQNSPALWRELNNPAQPAVLLYPGENAIDLSR
                     ERMPGPVTLVVVDGTWSNTKKMVAKNPCLAALPRVAFQPPRPSEYRIRREPRVDFVST
                     IEALMFALGALEEEPRRFEALLLPFRKLIDSQIECATKNKTPRRRRAVRRKSEPRKLP
                     ECLWERPEDLVCVVGEANAWPVREATQRSAYPDELIQWVALRPSTGESFQALAAPRNP
                     LAPSTASHLELPADELLRARELPELMRGFREFLRPNDVLCAWGCYSLALYCGAGGALT
                     EPSLDLRAISKAAMRQPVGTLEDFAASLSVSARAPLGTGRARRRVVELGRVVEFLASG
                     GGS"
     gene            4998..5357
                     /locus_tag="VFQ61_23090"
     CDS             4998..5357
                     /locus_tag="VFQ61_23090"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HET9957411.1"
                     /translation="MTQSLYERIGGEAAVDAAVDRFYRKVLSDDRISEFFDTTDMDRQ
                     RAKQKAFLTYAFGGPNQYSGKDMRAAHAKMKLNEQHFDAVVEHLAATLRELEVPEELI
                     AEVAGVATSVKQDVLNR"
     gene            5463..6473
                     /locus_tag="VFQ61_23095"
     CDS             5463..6473
                     /locus_tag="VFQ61_23095"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9957412.1"
                     /translation="MSEEIRISFEGRSYLLEEGESVLDCLLRNRESLDSYCRSGVCQS
                     CVVKARPHSRDAVPARAQVGLKEAAKRAGLMLACVCRPTQPLELERAEALPRYATRIH
                     DVRPLSPSVLRVELEVPSGFEYQAGQFIQLERPQDGLMRPYSLASLPSDPCLELHVAR
                     LENGAMSGWLESAVGQPVTIKGPLGECSYFDGEPERPLLLAGTGTGLAPLLGVIRAAA
                     RARHQGPITLLHGARDAAGIYHAEKLARLRAAIPNLRVLAVALEGPLPSDPGLEWRSG
                     ALDQQLFSEYAQLDEHRLYFCGNPELVGRLKKRAYLAGASLARIHSDPFLAPTQAPQA
                     RS"
     gene            6553..6996
                     /locus_tag="VFQ61_23100"
     CDS             6553..6996
                     /locus_tag="VFQ61_23100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET9957413.1"
                     /translation="MQLTVHTDYAFRTLIALATIAPELTTVGELSERYDISANHLSKV
                     VQRLSELGYVETLRGKAGGVRLAAAPSSIRVGDVVRLMEPELGLVECLRGGAPGACVI
                     SPACRLKSVLTTALDSFLHVLDGYTLADLVKNRGRVEKLLELRVP"
     gene            7166..8650
                     /locus_tag="VFQ61_23105"
     CDS             7166..8650
                     /locus_tag="VFQ61_23105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957414.1"
                     /translation="MKTKALSIGLRVVLFGAFGASLPGACSVQDRSNVVFASGGEDDG
                     TGGRTTGGATTSSGGRRTSGGSANSGGDAAGGQAGANDAGATQAEAGGGAGAGASPSQ
                     AGATSAGATGVVMDPAACAESLLRGTGKPLAESSTQDGTDAHKGSCGSGTGNDVVFDW
                     LVPATDYYSINTIGSDFDTVLYTLPSCEGKELACNNNTGTSPQSEILSHFKKDERVLL
                     VVDGNSGDQGSVALNTERITCPDTELTGQPLPATLSTSGGPNTHKGTCGGDGTPEKSY
                     HWTAPQAGIYRFTASSTEFVPSLYVERGPRCGGELLGCSQASGKTTTSYPATVSRYLQ
                     AGQVVNLIVDGGSGFFTLDAKRVDAGTTCGTTALPGDTPVTLKDTRQAGPVTQSCGPA
                     GSIDGVGSGPFPFSSVTYKMDVNFGQSTSCSYQVQANGPIVVSLIRGTCSGSEARCVA
                     STESGGTYTAKFSLGAADNGTYTLLIQNGSLREIEFTLTFACIA"
     gene            complement(8593..10062)
                     /locus_tag="VFQ61_23110"
     CDS             complement(8593..10062)
                     /locus_tag="VFQ61_23110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957415.1"
                     /translation="MPHYDPSTGELVVRIVYDGSGEAGKTTSIQGLCSLVSLQRRGPM
                     QSPGAMGRRTEYFDWLDFSGGFIDGRRVRCQLVSVPGQIEFSRRRERLLHSADAVVFV
                     LDSRPDALEESRERLVEARQTIAALPGPPVGMVLQANKRDVPEALPIPALTERLELDS
                     NVAVVPTVAVNGDGLIHAFVLATRLATDRARAQLSEGLPALPEEAASPAALHAAMVAL
                     EHSAGSSGSAKGDPWLDEAPFQSARVRNPVPEGWKCSLHAGLDISAGHVWPPVKGRAL
                     LTRASVEPFAQPEFLAPWAPSEAFEVRSADDWAFHSLPGWSFASESEARACLLSVVRS
                     QMRFVDLIPEGRALFLSSDGERSRIWLLSPPEVETLRQSFLRVVRERDGEALEVIRSC
                     VRGLDTRLGDGHGMSLDDVCVTGKVSILALPQASSERRNSEGPPTANPEPRAGLYAQL
                     GELERAAVARDPFIKHWLWPVYAMQANVRVNSISRREPF"
     gene            complement(10094..11158)
                     /locus_tag="VFQ61_23115"
     CDS             complement(10094..11158)
                     /locus_tag="VFQ61_23115"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HET9957416.1"
                     /translation="MAESDSDKTEEATPERRRKAREEGQFPRARDTGAVAATVGIVLT
                     ISALGGDYWNLLKAFSERCFSEGLAYRGGGIGFLVRELLHVLVVGTVPLAMVAAVCGI
                     LAGFAEAGFHPNLDLVAPKFERLDPVGKLGQMFSPKSALTSTALSLLRVGVVAWVTYS
                     ILKGEFGLLSRLTRTPVFSAAREVYAVWLRLALWSTVALALLAAVDYVTAWFRHEAQL
                     RMTPQEIKDEMKQQEGSPQIRARQRARARELLRRGVRKAVKEASVIVTNPTHIAIVLR
                     YKASEGAPVVTGKGYDDVAKHIRELARDYDIPIVENKPLARALAEKVKVGRVIPVDYY
                     VAVAEILAFVFRAKRRGVKA"
     gene            complement(11239..12039)
                     /locus_tag="VFQ61_23120"
     CDS             complement(11239..12039)
                     /locus_tag="VFQ61_23120"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HET9957417.1"
                     /translation="MSSAAFDPWLRAQACAFFLALVRISAMLVTAPLPWSTAPSRTRV
                     ILALLLTVCAYDRSPEWEALGANVLRSAASVGGEFMLGAAIGLVVRTTVAAVEIAADQ
                     MATSMGLGAAQMFDPEMHATSNVLSQILRYLAMFVAVSVGFHRLVLLATIQSFKIIPA
                     GSVLAPESFAPSLSTLVSTALSTGVRLAMPIVAVLFMTQVALAFISRAAPAMQVFSVG
                     FAVTLGVGGLVLLMALPDFTFELVADMSQLESRLSVLFSNLLPRAAPP"
     gene            complement(12073..12342)
                     /locus_tag="VFQ61_23125"
     CDS             complement(12073..12342)
                     /locus_tag="VFQ61_23125"
                     /inference="COORDINATES: protein motif:HMM:NF013480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HET9957418.1"
                     /translation="MTVDGAVELFKNAVMVCGMVIGPALAVALVMGLVVGVLQAATQV
                     NESSIAFLVKLLAVGATLAVLGSWSMQKLVDYTSRTIASISNVTR"
     gene            complement(12339..13016)
                     /gene="fliP"
                     /locus_tag="VFQ61_23130"
     CDS             complement(12339..13016)
                     /gene="fliP"
                     /locus_tag="VFQ61_23130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01103.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HET9957419.1"
                     /translation="MTPIGPPPSAAQALESMGLAPSLRILLVLTLLALLPAILLSCTS
                     FVRTVVVMSFVRQGIGSMQSPPSQVIIGLSLFLTAFTMAPVAREVQRVAIDPYMAGQL
                     TDMAALEAGIVPVKTFMLKQTREADLKLFYDATDTALPKTPEDVPMRLAMPAFVVSEL
                     TTAFHMGVIILLPFLVVDLAVASLLMSMGMMMVPPATLALPIKLLLFVLVDGWNLVAG
                     SILRSYT"
     gene            complement(13013..>13697)
                     /locus_tag="VFQ61_23135"
     CDS             complement(13013..>13697)
                     /locus_tag="VFQ61_23135"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HET9957420.1"
                     /translation="AKGTSDSVSVLRFTLFGVVLFGLSMYALYRHQRGRMGRGTAAQR
                     SALTLEGAVRVGPRAQVVVVNVAGRKLLLGVTESEVSRLAWLDDGMEPEAESEPERAE
                     APAPRAFTAVEPRHQPAFETRRLAAPATAPTPDQTAPRSTAAASAAANGAGKNAAPKR
                     FRDALLGALGHKTTDAPEPAAVAIAEATQDVVSRSRTAPIAAPPGAPEMVDVEGQARG
                     LVMRLQKRA"
BASE COUNT         2565 a         4395 c         4385 g         2352 t
ORIGIN      
        1 ggttggctct tcttcttgac ctgctccgta gtgctggcct tcgcggcctt ctgggagctg
       61 ctcgagtggt gggtaacgct gctggttgcc tccgacacgg ggcaagcctt cctcggctca
      121 caaggcgaca tctgggatgc tcagtgggat atgctgctcg ccggggttgg cgcggtcgtg
      181 gcgctgaccg cctttggacg ttttcatgac cgctcgatgg ctagagttcc ctttcctcac
      241 agggttcaag cgtcgacacc gccagcacgg ctcgcctgat gtcgagccgt ttctctgctc
      301 cgcacctgac acgggtgtga agcggcctac gaaacggcac ggaaaccgac accggtacag
      361 cttggcctca ggcgcatttc tgcctcgtgg ccgatacttt cgcggtaagc ttcggcatgc
      421 gctacccgag gggagacgat cgaacgccga ggcatttcag cgaatctggt cttcgcgtcc
      481 agaactcgct ggatagagcc gagcgcgaag agcaaaagcc acccggtagt ggcgagtttg
      541 tacgggcccg gctggaactc aagctcgacg ctctgcagct cgagcgccgg taccgcgaca
      601 gcctcaagtt gattgagctg aatcgggcac tcgtgggcgc acacgacttc gaacaggtgg
      661 cggcgctggt ttgctccgcg gtccgggcgc tcaccggagc cgacggcgcc acgttcgtgc
      721 ttcgcgaggg cgaccaagtt cactacgcgg cggaagatgc gatctcaccg ttgtggaagg
      781 gccagtcctt tccgatcgag cgctgcatct ccggttgggc gatcttgcat cgggcagtgg
      841 cggtcatcga agatatcttt tcggacccgc gtattcccgt cgaggcttac cgccccacct
      901 tcgtgagaag cctggtgatg accccggtcg ggccaggcgc tcccgtggct tcgattggcg
      961 cgtattggag ccgtcgctat cgagcgagcg ggtatagcct cgagctactg gacgcgatcg
     1021 cgagcgcggc cgatttggcg ctgagtagcg tgcgcgccta cgaggaatcc gaacgagccc
     1081 gattggaagc ggcgcgagcc aacgagctca aggatcagtt tctcgcgact ctatcccacg
     1141 agctgcgcaa tccgttgagt agcatggtgg cctattcgga gttgctcctc cggagctccg
     1201 atactcggca gataccgttg gtacggaccg cggcggattc gatccatcgc aacgccaaag
     1261 cccaggcgca gatcattcat gatctactgg acttctctcg cattcaaacc gggaagttcg
     1321 cactggagcg gcagctcatc tcgctcgctc cgctggtcgg cgaagcggtc gaatccgtgc
     1381 gggctcgagc gcaagaaaag cgcatcaagc tagatgtaga tctgcccgcc gaacccttcg
     1441 tctgccacgc cgacccggtg cggatccaac agatcgtctg gaacctcgtc agcaacgcgg
     1501 tgaaatttac ggcgcaaggc ggacgcgttt gcgtgcacct cacgcgagaa ggcgctcagg
     1561 cccagctcgt tgttcaagac aacggccagg gcatcgcccc cgagttcctg ccgcatgtct
     1621 tcgagatgtt tcgccaagga gatgaacaca agccgggacc gcagggcggg ctcggcattg
     1681 ggttagcgct cgtcaaacag cttgcggaat tgcacggggg ctccgtcgaa gcgagttcgg
     1741 aagggctcgg atgcggcgcg cgcttcgtcg tgcggctggc gctccagccc gacacggctc
     1801 cgcatcattc gagctctgcg ggcgggcgtt ccccgtcgca cgcagccggc ccaagcctac
     1861 ctggcgctcg catcttggtc gtggatgaca ccgaggagtt cctggaggtc atcgcgctgc
     1921 tgctcggcaa cgagggagcg caagtcacca cggcgcggag cgcagaggaa gctttgaaga
     1981 ccgcgcaatc gcatcatttc gatctggttg tgtcggatat ctcgatgccc gacgtagacg
     2041 gctatcagct ggttcaagcg ctgcgactgc tgccctcctt tgcatccact cccaccatcg
     2101 ccctgaccgg cttcggtcgg caagaagacg tcgagcgcac ccgctgcgcg gggttttctg
     2161 cccacctgac caaacccgtc cacatcggcc acctggtgga gcttgcgcga gtattgatcc
     2221 caccacgcgc gccacagtga aacttgcctc gcgcccaccg agtgtcgcgg gtattggcgt
     2281 ggcttgagtg ctgcgcgctc ttggcattac tcgtgcagac tctctgatcg gcgaagggtc
     2341 cccccttggg ctctcgtggt gactcgcaca ctccatggaa cgctggatcg aaagtctgca
     2401 acgccgcgcg gcaactcaag aggatggatt accgctctct ggaggcttac ctctcgcgga
     2461 gttgtttccg cagaaagagc ttctggcttg tcacgccgct ctcagcctcg aggcggactc
     2521 gagcgcgctc cagtacacgt ggcctgaggg cagcgaggcc ctacgcacgt ggattgcggc
     2581 gcgattgcgc aagcgcggcg caaacaccac acccgcggac gtgatcataa cgaccggagc
     2641 ccagcaggcg ctcgtcatgc tttgcgcggt gctgaagctg gagggacggc ggatcggagt
     2701 ggacgcggcg acctactcgg gtgcgttgga tgtctttcgc cgagcgcgag ctttgcccgt
     2761 ggtctcgact cgggcggcgg caatctacgc gatccccggc tccagtaatc ccgaaggcag
     2821 aggcctcggc gcgccccggc gcgccgagct gctggaggcg ggctgtccca tcattgccga
     2881 cgaagcctac gccgaactcc ggtacgatgg agtgctcgaa cgtccactga ttgccgacgc
     2941 gccggaccga gtattccacg tcggcaccct atcgaagacg ctgtgtccgg gcttgcgctt
     3001 gggctggctg gttgccccat cacagctgcg ttcgccgctc ctggacgcca agcgagatct
     3061 cgacctgcag gcaccgagtc tctctcaagc actcgtcgag cggctgctga acgacttcga
     3121 ttgggacgct cacttggagg tccagcgacg tgaatatcga gcccgcatgg caacgctgat
     3181 ggggtccctg cgcaggcacc tgccagagct agagttcgag gaacccgagg gcgggttcac
     3241 gttattcgtg cgttacgcag gctcgccagg tcagagcccg tcagggctgg atgaacctct
     3301 ccttctcgac tgcgctacgc gccacgggac gagcttcgat ccgggcagca tgtttcaggc
     3361 ggagcccgcg cctttggcgc tgcgactatc accatgcggc gtaggcaaag aacagatcga
     3421 ctccgccgta cagcgactcg cggccgcttt ctcggaggcg cgcgaccggg tcgcgacgaa
     3481 gacggctcag acctctggag ccgccccgtc agctccctcc accggaggcc aagaactcga
     3541 cgactcgccc cagctccacg actcgcctcc gggcgcgacc cgttccgagc ggagcacggg
     3601 cactgacgga taagctggcg gcgaagtcct ccaaggtgcc cacgggctgg cgcatagcgg
     3661 ctttgctaat ggcgcgcagg tccagactgg gctccgtcaa ggctccgccc gccccgcagt
     3721 aaagcgcgag tgagtagcaa ccccatgcgc aaagcacgtc attcggccgc aggaactcgc
     3781 ggaagccgcg catgagctcc ggcaactcgc gtgctctcag cagttcgtcg gccggcagct
     3841 ccaggtggga agccgtgctc ggagcgaggg gattgcgcgg agcagcgaga gcctggaagc
     3901 tttccccggt gctcggacgc agcgccaccc actggatgag ctcgtccgga taggcggacc
     3961 tctgcgtcgc ctctcggacg ggccaggcgt tggcctcccc gactacgcag accaaatctt
     4021 cggggcgttc ccaaagacat tcgggtagtt tgcgcggctc ggatttcctc cgcaccgctc
     4081 gacggcgccg aggcgtcttg ttcttcgtcg cgcactcgat ctgcgagtcg atcagcttgc
     4141 ggaatgggag cagcagggcc tcgaatcttc gcggttcctc ttcgagcgca cccaaggcga
     4201 acatcagagc ctcgatcgtg gacacgaaat cgacgcgcgg ttcccgccta atccgatatt
     4261 cgctcggacg aggcggctgg aatgcgaccc ttggcagagc cgccaaacac ggattcttgg
     4321 ccaccatctt cttggtattg gaccaagtgc catccaccac caccagggtc actgggccag
     4381 gcatccgttc acgcgatagg tcgatcgcat tctcgccggg atagagcagc accgcgggct
     4441 gcgcgggatt gttcagttca cgccacaacg ccggcgagtt ctgccaatcg aatccaacca
     4501 agagctcgga attgggtagg ctgaggtggg ccatacgggc cgtgccgatc ggcatatccc
     4561 gctctcgcgg gtgttggagc aagagcactc gagtccgagt ttggatggtg cgcagctcgc
     4621 tgcagtagca ggtcacccgc ggccggcggc aacccgggca gacccagcgc ggttggaact
     4681 cggtgctgtc gtgcattcgg cgcggcacta gcacgttgcc ctgccgctgg cgatggtgct
     4741 gcagtcagtg gctgaaggac cgggggtgag ttcgaccgaa cccctagggc ttggagtgcg
     4801 accgccgctc gcgctgtcgc gagtagcggt cgcatgtgcg gagttcgtct cggtcttttg
     4861 ttcgacggac gccactgaat aagtgccacg acgctgccgt tcactgccga gtcttactgt
     4921 cgcttgacat gccgcacctt aaacaggtat ttcacatacc actttaccca gagcatcttt
     4981 gcaacgaagg gattcgcatg acgcaatctt tgtacgaacg tattggcggc gaggctgcgg
     5041 tcgacgcggc ggtggaccgc ttctatcgca aggtcctgag cgatgatcgg atttccgagt
     5101 tcttcgatac caccgacatg gaccgtcagc gcgccaagca aaaggccttc ctgacctacg
     5161 ctttcggtgg acccaatcag tattccggca aggacatgcg cgcagcccac gcgaaaatga
     5221 agctgaatga gcagcatttc gacgcggtcg tcgagcactt ggcggccacc ttgcgcgagc
     5281 tcgaggtgcc ggaggagctg atcgcggagg tcgcgggtgt ggccaccagt gtgaagcaag
     5341 acgtattgaa tcgctgatcg ccttcgacgc gatcgagcct actgactgcg gattgaacgc
     5401 cgcgcgcggg gcacgcgcgg cgacggatcg ggacttgtag ttccagctcg gatcttactg
     5461 cgatgagtga agaaattcgg atttcgttcg agggtcgttc ctacctgctc gaggagggcg
     5521 agagcgtcct ggattgtttg ttgcgcaacc gagagtcgct cgactcctac tgtcgcagcg
     5581 gggtctgcca gagctgcgtg gtcaaggcgc gcccgcatag ccgtgacgcc gttccggctc
     5641 gcgcccaggt cggcctcaag gaggctgcaa agcgggcggg actgatgctg gcgtgcgtgt
     5701 gccgcccgac tcagccgctc gagctcgagc gtgccgaggc gctgccgcgc tacgcgactc
     5761 gaattcacga cgtgcggcct ctgtcgccga gcgttttgcg cgtcgagctg gaggtaccga
     5821 gcggcttcga gtaccaagcc gggcagttca ttcaactcga acgcccccag gatggcttga
     5881 tgcggcccta ctctctggcc tcattgccga gtgacccctg cctggagctg cacgtggcgc
     5941 gtctggagaa cggcgccatg agtggctggc tcgaatcagc ggttgggcaa ccggtgacga
     6001 tcaagggtcc gctcggagag tgttcctact tcgacggcga gcccgagagg ccactgctac
     6061 tggccggaac gggcaccggt ttggcgccac tgctcggcgt catccgcgcg gcggcgcgcg
     6121 cgcggcatca gggtccgatc accctgctgc acggcgcccg agacgcagcc ggcatctatc
     6181 atgccgagaa gctcgcgcgg ctgcgagcgg ccattcccaa cctgcgcgta ctggcggttg
     6241 cgctggaagg accattgccc agtgatcccg gcctggagtg gcgctcgggt gccctggacc
     6301 agcagctctt ttccgagtac gcccaattgg atgaacaccg gctgtacttc tgcggaaacc
     6361 ccgagttggt ggggcgactc aagaagcgcg cctatttggc cggagcgagc ctggcgcgga
     6421 tccacagcga tccgttcttg gcgcctacgc aggctccgca agcgcgcagc tgacagcata
     6481 ctcggtggca cgtcgccgag cgcatgcgcg tcgagcgacc gcaccgctga gtgttcacgg
     6541 gaggagagct ccatgcagct gacggtacac accgactacg cgttccgaac cctgatcgcc
     6601 ctggccacga ttgcgcccga gctgaccacg gtgggcgagc tgagcgagcg ctacgacatc
     6661 tccgcaaatc acctatcgaa ggtcgtgcag agattgtcgg aattgggcta cgtggagact
     6721 ttgcgcggca aggccggcgg cgtgcgcttg gcggcggcgc ccagctcgat ccgggtgggg
     6781 gacgtcgtac gtctgatgga gcccgagctc gggctggtgg aatgcctgcg cggcggcgcc
     6841 cccggagcgt gcgtaatctc cccagcttgc cggctcaaat ccgtgctcac gacggcgctc
     6901 gatagcttct tgcacgtgct cgacggatat acgctcgcgg atctcgtgaa gaaccggggt
     6961 cgggtcgaga agctgctcga gctgcgcgtt ccctgagcgt ctcgaggaag cgggctcctc
     7021 gagccagtag atagggccct ggatgtaaaa accacgattg aagtgcggtc taggaaacgc
     7081 tctcccgggc cccctgcccg aagaagaggg cgaggcaatt ggggccagtt gagccgcata
     7141 accgggcagt gctatcctgc cagggttgaa aaccaaagca ctctccattg gtttgcgcgt
     7201 agtactgttc ggcgccttcg gtgcttccct cccgggcgcg tgtagcgtgc aggatagaag
     7261 taacgtggtg ttcgcgagcg gcggcgagga cgacggcact ggcgggcgaa cgacgggcgg
     7321 cgctacgacg agctccggtg ggcgtcgcac gagcggaggt tctgctaact ccggcgggga
     7381 cgcagcgggc ggtcaagccg gcgcaaacga cgccggtgca acccaagccg aggcgggtgg
     7441 cggagccgga gctggagcga gcccttctca agccggagcg acgagtgcag gggccacggg
     7501 cgtagtcatg gatcccgcgg catgtgcgga gagcctactg cgcggaaccg gtaagcccct
     7561 cgccgaatcg tccactcaag acgggaccga cgcgcacaaa ggcagctgcg ggtcgggcac
     7621 gggcaacgac gtagtgttcg attggctagt tcccgccacg gactactaca gcatcaacac
     7681 catcggctcg gacttcgata ccgtgcttta cacgctcccc tcgtgtgaag gcaaagagct
     7741 ggcctgtaac aacaacacgg gcacttctcc gcagtcggag atcctgagtc acttcaagaa
     7801 agacgagcgc gttctcttgg tcgtcgacgg gaactcgggc gatcaaggct cggtcgcgct
     7861 gaacaccgaa cgcatcacct gcccggacac cgaactgacg ggacaaccgt taccggccac
     7921 gctgtccacg agcggcgggc ccaacacgca caagggcacc tgcggtggag acggcacgcc
     7981 agagaagtcc tatcactgga cggcacctca agccggcatt taccgcttta cggcgagctc
     8041 gaccgagttc gttcccagcc tctacgtcga acgcgggcca cgttgtggtg gtgaactgct
     8101 cggctgcagc caagcgagcg gcaaaacgac gacgagttac cccgccaccg tcagtcgcta
     8161 ccttcaggcc ggtcaagtcg tgaacttgat cgtggacggc ggctccggct tcttcacgct
     8221 cgacgcgaaa cgggtggacg cgggcacgac ctgcgggacg accgcactgc cgggtgacac
     8281 gccggtcaca ttgaaggaca cgcggcaggc ggggccggta actcaatcgt gtgggcctgc
     8341 gggcagcatc gacggcgtcg gcagcggccc cttcccgttc tcgtccgtca cttacaagat
     8401 ggatgtgaac ttcgggcaga gcacgagctg ctcctaccaa gtgcaggcga acggtccgat
     8461 cgtggtctcg ttgatccgag gcacttgctc gggctccgag gcccgctgtg tcgccagcac
     8521 cgagtcgggt gggacgtata ccgccaagtt ctcgctcggt gccgcggaca acggcactta
     8581 caccttgctg attcaaaacg gttctctgcg agagatcgag ttcactctta cgttcgcctg
     8641 catcgcgtaa acgggccaaa gccagtgctt gatgaacggg tcgcgcgcca ccgcggcccg
     8701 ctcgagctcc cccagttgtg catagagccc cgcgcgcggc tccggatttg cggtgggagg
     8761 cccttcgcta tttcgccgct cgctcgaggc ttgaggcaag gccagaatgg aaaccttgcc
     8821 tgtcacgcat acgtcgtcga gcgacatgcc gtggccatcg ccaagccgcg tatcgagccc
     8881 ccgcacgcag gatcggatca cctcgagcgc ttcgccgtcg cgctcgcgca ccacccgcag
     8941 gaatgactga cgcagcgtct ccacctccgg cggagacaat agccagatac gcgagcgctc
     9001 gccgtcgctg ctcagaaaca gcgcgcgccc ctccggaatg agatctacga agcgcatttg
     9061 actcctgacc acggagagca agcaggcgcg ggcttcggat tcactcgcaa acgaccagcc
     9121 cggcaacgag tggaaggccc agtcgtcggc gctgcgaact tcgaaagctt ccgacggcgc
     9181 ccaaggcgcg aggaactcgg gctgagcgaa gggctccacg ctggcgcgcg tgagcaaggc
     9241 cctgcccttc actggcggcc acacgtgccc cgccgaaata tctaggcccg cgtgcagcga
     9301 gcacttccaa ccttccggca ccggattccg cactctcgca gactggaagg gcgcctcgtc
     9361 gagccagggg tcgcctttgg ccgaacccga actgcccgcg ctatgttcga gtgcaaccat
     9421 cgcggcatgc aatgccgccg ggctcgccgc ttcttctggc aatgcgggaa ggccctccga
     9481 gagctgcgcg cgggcccgat ccgtggcgag ccgcgtggcg agtacgaaag cgtgaattag
     9541 gccatccccg ttgacggcca ccgtagggac caccgccacg ttcgagtcga gctcgagccg
     9601 ctcggtgagt gctggaatgg gaagagcctc cgggacgtcg cgcttgtttg cttgcaagac
     9661 catgcccacg ggcggcccgg gcaacgcggc gatcgtctgc cgcgcttcca ccagccgctc
     9721 acgtgattcc tcgagcgcat cgggacgaga gtcgagtacg aataccacgg cgtcggcgga
     9781 gtggagcaag cgctcccgac gacgactgaa ctcgatttga ccgggcacac tcactaattg
     9841 gcagcgcacg cgacgaccgt cgatgaagcc tcccgaaaag tcgagccaat cgaaatactc
     9901 cgtgcgacga cccatggcgc ccgggctctg catgggcccg cgacgttgca gcgaaacgag
     9961 cgagcacagg ccttgaatac tcgtggtctt gcccgcctcc ccgctcccgt catacacgat
    10021 tcgaaccacc agctcgcccg tactcgggtc atagtgaggc atccgctcag tttagcgccg
    10081 atcagccgtg gaatcaggct ttgacgccgc gtcgtttcgc tcggaaaacg aaggcgagga
    10141 tctccgcgac ggccacgtag tagtcgactg gaatcacacg accaactttg accttctcgg
    10201 ccaacgcgcg tgccagcggc ttgttctcta cgatcgggat atcgtagtcg cgagccaact
    10261 cacgaatgtg cttcgccacg tcgtcgtagc ccttgccggt cacgacgggc gcgccctccg
    10321 aagctttgta acggagcacg atcgcgatat gagtcgggtt ggtgacgatg acgcttgcct
    10381 ctttgaccgc cttccgcacg cctcgcctga ggagctcccg cgcgcgcgcc cgctgacgcg
    10441 cgcgaatttg cgggctgccc tcttgctgct tcatctcatc tttgatttct tgcggcgtca
    10501 tgcgcaactg cgcttcgtga cggaaccacg ccgtgacgta gtccaccgcc gccagcagtg
    10561 cgagcgccac ggtggaccat agggcgagac gcaaccacac cgcgtacacc tcgcgcgcgg
    10621 cgctgaacac cggtgttcgc gtgagccgcg agagcaggcc gaactcaccc ttcaagattg
    10681 aataagttac ccacgccacc actcccaccc gaagtaggga aagagcggtg ctcgtgagcg
    10741 cagacttggg cgagaacatt tggcccaact tcccgaccgg atcgaggcgc tcgaacttcg
    10801 gggcaaccaa atcgaggttc gggtggaacc ccgcctcggc aaaacccgcg agaatgccac
    10861 agacggccgc gaccatcgcg agcggcacgg taccgacgac gagtacgtgt aggagctcac
    10921 gcaccaaaaa cccaatccca ccgcctcggt acgcgagacc ttcggagaaa cagcgctcgc
    10981 tgaaggcttt tagcaagttc cagtagtcac ccccgagcgc cgaaatggtc agcacgattc
    11041 cgaccgtcgc ggctacggca cccgtgtcgc gcgcacgagg aaactgcccc tcttcgcgcg
    11101 ccttgcgtcg ccgctctggg gtggcttcct ctgtcttgtc ggagtcgctc tcggccaatc
    11161 gagttactca gttgaaggga tgcagccggt ccaaagagag ccaccggagc gctcgtcacc
    11221 gaaatcgaga gcagtcactc acggtggcgc ggcgcggggt agcaggttgg aaaacagcac
    11281 tgaaagtcga gattcgagct gggacatgtc tgcgacgagc tcgaaggtga agtcgggtag
    11341 agccatcaag agcacgagcc cgcccacccc gagcgtcacg gcgaagccga cggagaagac
    11401 ctgcatcgcg ggtgcggcgc gagagatgaa tgcgagcgcg acttgggtca tgaaaagcac
    11461 cgccacgatc ggcatcgcca aacgcacgcc ggtcgagagc gccgtcgaca ccaacgtcga
    11521 gagcgagggg gcaaagctct cgggcgccaa aacggatccc gcaggaatga tcttgaagct
    11581 ctggatggtg gccaaaagaa ccaagcgatg aaatcccacg ctcaccgcta cgaacatcgc
    11641 gagatagcgc aggatttgtg agagcacgtt gctcgtcgca tgcatctcgg gatcgaacat
    11701 ttgcgcggcg ccgaggccca tactggtagc catctgatcg gctgcgatct cgacggcagc
    11761 aacggtggtg cgtaccacga gaccaattgc cgcccccaac atgaactctc ccccgaccga
    11821 agcggcgcta cgcagcacgt tcgcccccag cgcctcccac tccggggaac gatcgtaggc
    11881 acagaccgtc agcaggagcg cgaggatcac ccgcgttcga ctcggggccg tgctccaagg
    11941 cagaggtgcc gtaacgagca tggcggaaat ccgcacgagc gcgagaaaga acgcgcacgc
    12001 ttgggctctc aaccagggat cgaatgcggc ggaggacacg tgacaggtgg gtcagtgcga
    12061 cacgacggaa actcagcgag tcacattgga gatgctggcg atggtgcggc tcgtgtagtc
    12121 gacgagcttc tgcatggacc aggagccgag cacggccaag gtggcgccga ccgcgagcaa
    12181 ctttaccagg aaggcgatgc tcgactcgtt gacttgggtc gcggcttgaa gaacgcccac
    12241 cacgagcccc atcaccagcg cgacggccag cgcggggccg atcaccatgc cgcaaaccat
    12301 gaccgcgttc ttgaatagct cgactgcacc atcgaccgtc acgtgtagct cctcaagatc
    12361 gagcctgcga ccaggttcca gccatccacc aacacgaaca gcagcagctt gatcggcagt
    12421 gccaaggtcg ccggtggaac catcatcatg cccatgctca tgagcagaga agcgaccgcc
    12481 aaatccacga ccaagaacgg caatagaatg atcacgccca tgtggaaggc ggtggtgagc
    12541 tcggatacta cgaaggccgg catcgccaaa cgcatcggca catcctccgg ggtcttgggt
    12601 agcgcagtgt ccgttgcgtc gtaaaacagc ttcaggtcgg cctcgcgcgt ctgcttgagc
    12661 atgaacgtct tgaccggcac gatcccggcc tcgagcgcgg ccatgtcggt gagctgcccg
    12721 gccatgtacg ggtcgatggc cacccgctga acttcacgcg ccaccggcgc catcgtgaag
    12781 gcggtcagga acaagctcag cccgatgatc acctgactgg gcgggctctg catcgatccg
    12841 atcccttggc gcacgaacga catcactacg acggtgcgca cgaaggacgt gcaagagagc
    12901 aggattgccg gcagcagagc cagcaacgtg agcacgagca ggatgcgcaa agaaggcgcg
    12961 agccccatgg actcgagcgc ctgcgccgcg gaggggggcg gaccaatcgg agtcatgcgc
    13021 gcttctgcaa acgcatgacg agaccacgcg cctgaccctc tacgtcgacc atctcgggcg
    13081 cacccggcgg tgcggcgatg ggcgcggtac gagaccggct gaccacgtcc tgggtcgcct
    13141 cggcaatcgc taccgcggca ggttcgggcg catctgtcgt cttgtgtccg agcgcaccga
    13201 gcaaggcgtc acggaagcgc ttgggagcgg cgttcttccc cgcgccattt gccgccgcgc
    13261 tcgccgccgc cgtcgagcga ggcgccgttt gatcgggcgt gggagcggtc gcgggcgccg
    13321 ccagtcgacg cgtctcgaac gcgggttgat gccgaggctc caccgccgtg aaggctcggg
    13381 gcgccggggc ttcggcgcgc tcgggttcgg actcggcttc aggctccatt ccatcgtcga
    13441 gccaggcgag tcggctgact tcggactcag tcactccgag tagcaacttg cgcccggcca
    13501 cgttgacgac caccacctgc gcgcgcggcc ccacgcgcac cgcgccctcc aaggtgagag
    13561 cggagcgctg agctgccgta ccgcgaccca tgcgaccgcg ctgatgacgg tacagcgcgt
    13621 acatggatag accgaagagc acgaccccga agagtgtgaa gcgcaaaacc gaaaccgagt
    13681 cggaggtgcc cttcgct
//
LOCUS       DASTKU010000664         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1914307, whole genome shotgun sequence.
ACCESSION   DASTKU010000664 DASTKU010000000
VERSION     DASTKU010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914307"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFQ61_23140"
     CDS             complement(<1..227)
                     /locus_tag="VFQ61_23140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957421.1"
                     /translation="MPRAPLRELWYARHAKPVSSGVCYGHHDVPVIDLAPSAAVKLHE
                     LFPGSASPRRVWSSPSLRCWELACELAQLWGA"
     gene            complement(276..965)
                     /locus_tag="VFQ61_23145"
     CDS             complement(276..965)
                     /locus_tag="VFQ61_23145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957422.1"
                     /translation="MPSSSIGRAHSKVLLALLAGPLLSIFGSSACGSDAREEPSAPSG
                     GRAVIPPPAQSGSAPAGNQTGGRTNSGNSSGASSGRGGSANGGVSPNGGTRVSTGGTG
                     GTTSQVAQGGMPEPNWPVVPVGTDGVSPYLRECHGETVECQDPKSLTCLGIRDEKGVQ
                     GFACSNTCETNADCAAEATGVGAKPSCIDFAISRYCMLACTPDDSTNDCPSGMACYVY
                     PGTTAGYCLWR"
     gene            1180..2400
                     /locus_tag="VFQ61_23150"
     CDS             1180..2400
                     /locus_tag="VFQ61_23150"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9957423.1"
                     /translation="MAPSSIRRSPPASRNKKSFVNALGSVTLLTAAAWVAALAGACSS
                     DADSAGSPSTTTTTTATSRIPTSNSNPNGAAAGNSGLTPGSGCGESTPGACAGVSFEG
                     ERIPVDLYIMFDQSGSMLNDVGGMTRLDAVRQAVTTFLRDSGGAAIGVGIGYFGTQPI
                     GQVSCNAADYSRPAVPVSLNHELVIQSVSGREPTGETPTAAALTGACGYVSEYARSNP
                     GRAAAILLVTDGTPEAPVSCSQGGCCPTLDQAADAASSCAAGKTPIPTYVLGVGPNLD
                     GLHRIAAAGGTERAYLVDDRDVANSVLQALLQIRGAAQIPCDIEIPEPDPGLTLDYSK
                     INVLSSATSCAVQPFYYVESAADCGHELAWHYDNNAAPSRIQLCSQACEAVNQPSTRL
                     RVLLGCESVELPPR"
     gene            2481..>2992
                     /locus_tag="VFQ61_23155"
     CDS             2481..>2992
                     /locus_tag="VFQ61_23155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957424.1"
                     /translation="MQRLNSIHCAFTVLLLSLSASVGCGEADHGNGGPGSRAGASGVG
                     GGANSGRGGGNSGSGASMSGGGNTGSTTEAPPGFHGTPGSTGFVPLSAGCGPSTRLEC
                     SSQCASGAGNSTVLRPPATLCFVSDEDPSPADPIALIEQVIEETEGKRLVHLRVTFDP
                     AFVDNTYGENA"
BASE COUNT          559 a          988 c          922 g          523 t
ORIGIN      
        1 gccccccaga gctgagcaag ctcgcacgca agttcccagc aacgcaagct cggggagctc
       61 cacacgcgtc gtggagaagc gctacccgga aacagctcgt gcaacttgac cgcggcggag
      121 ggggcgagat caatcaccgg aacgtcgtga tgtccgtagc aaacacccga cgacacgggt
      181 ttggcgtggc gggcgtacca gagttctcgc aaaggggctc gaggcatcgg cgctgtggac
      241 ttaacaccgg attcggactt tgccagggcg gacatctatc gccacaagca atatcccgcc
      301 gtagtcccag gatacacata acaagccatg cccgaggggc agtcattggt tgaatcgtcc
      361 ggcgtacatg cgagcatgca gtagcgcgaa atcgcgaaat cgatgcacga aggctttgcg
      421 cctactcccg tggcttcggc ggcgcagtcc gcgttcgttt cacaggtgtt cgaacaagca
      481 aacccttgca cgcccttttc gtctcgaatg cccaggcagg tgagcgactt cggatcttgg
      541 cactcgactg tctcaccgtg gcactcgcgc agatatggag acacgccatc ggttccgacg
      601 gggacgacgg gccaattcgg ctcgggcatc cccccctgcg cgacctgtga agtagtgccg
      661 cctgttccgc cagtcgacac gcgcgtcccg ccgttcggcg aaactccgcc gttcgccgaa
      721 cccccacggc cgctgctcgc tcccgaggag ttcccgctat tggttctgcc acccgtttga
      781 ttgcccgccg gcgcggaccc cgattgcgca ggagggggaa tcaccgcccg tcccccgctc
      841 ggcgcggaag gctcctctcg cgcgtcagaa ccgcaagcgc tgctcccgaa gatgctcaac
      901 agggggcccg cgagcagcgc caaaaggact ttggagtgag cacgaccaat agagctagac
      961 ggcatcagac cgagccatgg gatcttccaa gcgcttttct gatctccacg cagtggccca
     1021 ctttcatccc ttgaaccagg gggacggatc caccggctcc gattccactc ccgcgacgcc
     1081 gtatgggcat ctggaccacg gtttctgatc gattcacctg atatttctca cgagaaaaga
     1141 atcagaaatc tcagccatcc gctccatcgg gaggtcgaga tggccccgtc ctcgattcgt
     1201 cgcagcccac ccgcgtcgcg aaacaaaaaa tcgttcgtca acgcgctcgg ctcggtgacg
     1261 ctcctgaccg ccgccgcttg ggttgccgcc ttggctggcg cttgctcgag cgacgctgac
     1321 tcggcgggct cgccgtccac gaccacgacc accaccgcga ccagtcgtat cccgacctcc
     1381 aactcgaacc cgaacggtgc ggccgcggga aactccgggt tgaccccggg aagtggctgc
     1441 ggggaatcga caccaggcgc ctgcgcgggc gtgagcttcg agggggaacg aatcccggtc
     1501 gacctctaca tcatgttcga ccagtccggc tcgatgctga atgacgtggg ggggatgacg
     1561 cggctcgatg cggttcgcca agccgttacg acgttcttgc gtgattccgg cggcgcggcg
     1621 attggcgtgg gaatcgggta tttcggaacg cagccaatcg gccaagtctc ttgcaacgcg
     1681 gccgactact cgcgacccgc cgtacccgtc agcctcaatc acgaattggt gatccaatcc
     1741 gtatcaggcc gagaaccgac cggagagacc ccgaccgcag cggccctgac cggagcatgt
     1801 ggatacgtca gtgagtacgc gcgcagcaac ccggggcgcg ccgccgcgat tcttctggtt
     1861 accgatggca ctcccgaagc gccggtgtcc tgtagccaag gggggtgctg cccgactctc
     1921 gatcaagccg ccgacgcggc ctcgagctgt gccgccggca aaacaccgat tcccacgtac
     1981 gtgctcgggg taggcccgaa cttggacggc ttgcatcgca tcgccgccgc cggcggcacc
     2041 gagagagcgt acttggtcga cgaccgagac gtcgcgaaca gcgtcttgca ggcgctgctg
     2101 caaattcgcg gagcggccca gatcccgtgc gacatcgaga tacccgagcc tgacccaggc
     2161 ctaaccctcg attattcgaa gatcaacgtg ctcagctcgg cgacgagctg cgcggtgcag
     2221 ccgttctact acgtcgaatc ggccgcagac tgtggccacg agctggcgtg gcattacgac
     2281 aacaacgcgg cgccgtcgcg aattcagctg tgttcgcaag cctgcgaagc cgtgaatcaa
     2341 cccagcactc gcctgcgtgt gttgctgggg tgcgagtcgg tcgaactccc cccgcgttga
     2401 gccactcagc gagtgatcga aaccgtctcg gcgcgagctc gtcgggactc gagataaaga
     2461 ctgcccggag caattcatcc atgcaacgac tcaattccat tcattgcgca ttcacggttt
     2521 tgctgctctc tctttccgcc agcgtcggct gtggcgaggc ggaccacggg aacggcggtc
     2581 ctggctcgcg tgctggagct agcggcgtcg gcggaggcgc caactccggt cgcggtggag
     2641 ggaacagcgg ctctggcgcc tcgatgtccg gtggggggaa cacgggttcc acgacggagg
     2701 cgcctcccgg attccacggt acgccgggca gcaccggctt cgtgccgctc agcgctggct
     2761 gtgggccgag cacgcgactc gaatgctcgt ctcagtgcgc gtctggagcc ggaaactcga
     2821 ccgtattgcg gcctcccgca acgctgtgct tcgtttcgga cgaggatccg agtcccgccg
     2881 atccgattgc cttgatcgag caggtgatcg aggaaaccga gggcaagcgt ttggttcatc
     2941 tgcgcgtcac gttcgacccc gctttcgtag acaacaccta cggcgagaac gc
//
LOCUS       DASTKU010000665         9364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1914359, whole genome shotgun sequence.
ACCESSION   DASTKU010000665 DASTKU010000000
VERSION     DASTKU010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9364
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914359"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /locus_tag="VFQ61_23160"
     CDS             complement(<1..1185)
                     /locus_tag="VFQ61_23160"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HET9957425.1"
                     /translation="MEAQQPHVSVGVSDVEQDAEHRLSASLRAALNLARAIAQEYRVP
                     GEIGTGLLLVAIVRTGRAQPKDASGPAELMKLIDSLGTHSLGSACDEFLERERRSTSG
                     GGPRYDELSPNLNGVLEAARHLPNHDGILAMRHVIVSLLTFRPRVGVSNAHRILDQAG
                     VQRATVHETFVDSLAARGPEPERDGWRALRPSASRPSASRTLRPEYWADVPSGRAADD
                     CLNLDATVSAVANLFASPELDGSLSLGVFGNWGSGKSFFMRRLRLAIDELTAQARAQA
                     EPWATPVYWPNIVQVEFNAWHYVDSNLWASLVAHLLQQLRDFKPGERSETQRTQLQKA
                     LSDLNLAADLHAAAERARAVAADAREVAERSLKKAEEDATEAERSLAAALGRSLWSYV
                     EEE"
     gene            1301..1933
                     /locus_tag="VFQ61_23165"
     CDS             1301..1933
                     /locus_tag="VFQ61_23165"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9957426.1"
                     /translation="MPVDAAFWDNIAEKYSRKPLANPDAFERKIAVTKARIKPTDIII
                     DLGCGTGSLALRLAPCAAHVHGLDISSEMVRIANEKALAQKIENVTFHVGAFDDTFTR
                     FGPESLDGVCAYNLLHLVEDRAAALARIHRLLKPGGFFISSNPCMGESKIPYRPILRV
                     MRWLGKAPLVEIFTKRTIEDEIRKAGFVDLDQPDVGAKPEISFIVAVKPR"
     gene            complement(2035..2484)
                     /locus_tag="VFQ61_23170"
     CDS             complement(2035..2484)
                     /locus_tag="VFQ61_23170"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9957427.1"
                     /translation="MVESKPVRRAAVPSDGLVDGLVRTSFFCMAVLNKIGAENDLSLT
                     QLRVCGILRDRRVRMAELAEYLGLEKSTMSGLIDRAEKRGLVARSPSPEDRRAIDVYL
                     TSEGAALVKDLYAQVRQSLAHLTSRLGASDQRQLEELLRRMLDAPGG"
     gene            2585..3526
                     /locus_tag="VFQ61_23175"
     CDS             2585..3526
                     /locus_tag="VFQ61_23175"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HET9957428.1"
                     /translation="MKAAVIRSFGKPPSYEDFSEPLPRGPEEIRVQVLAAGLHHLTRA
                     RADGSHYTSAQTLPMVPGVDGVGRGPDGKLRYFVVDGGQIGSMAEETVVEIDHSLVLP
                     RDCDPVTVAAAMNPAMGSWLALRCRVPFKKRQKVLILGATGNAGSMAVQVARHLGAAQ
                     IIAVGRDQQRLAKLGALGATDLVSLGDARLGALAREVDVVLDFIWGESSAQTLELLVR
                     DRADRARPLTWIEVGSVGGQTAAIPASALRASRLEILGSGIGSVPGREILKELPALVK
                     EITRGTFRIDAKAVPLRAVEQAWCEAAHMSERVVFTP"
     gene            4019..4264
                     /locus_tag="VFQ61_23180"
     CDS             4019..4264
                     /locus_tag="VFQ61_23180"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9957429.1"
                     /translation="MRSPSARKATSAQVALAWLLAQKPWIVPIPGTTKLPRLEENLSA
                     LRIELTPEDLRAIDTSAARLTVQGNRYPDELERLTGL"
     gene            complement(4339..4749)
                     /locus_tag="VFQ61_23185"
     CDS             complement(4339..4749)
                     /locus_tag="VFQ61_23185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957430.1"
                     /translation="MEGKLKNLKDLAANPQPTKAQFGIGGFSSWVILSLMVSLAVACG
                     AAEGDPIQAEPEVATETMTQRLSNVDTASGGPDAQGALLEATQEMQETQMSFNLQYLE
                     LQSQMQHENRSYTAVSNIMKTKHDTAKNSISNVR"
     gene            5454..6779
                     /locus_tag="VFQ61_23190"
     CDS             5454..6779
                     /locus_tag="VFQ61_23190"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9957431.1"
                     /translation="MSDSTLPVLTGGEQEGPPTLWGLELVFPPEDARRFRISSARGLA
                     IGRDADADVVIASHQVSRRHADIVCVGEELWVHDSGSLNGTHVNGERVIRRPLHEGDV
                     LRIGDRLAVVVRDDIEEMSSATVTQIASGFWAGSVLQAALANARKAARTDLPVVLQGA
                     TGTGKERAARAIHEWSGRAGAFVPINCAAIPEHLAEAELFGYRKGAFTGADRSAVGHL
                     RAAHGGTLLLDEVSDLPLPLQAKLLRVVEQSEVQPLGEARALPIDVRLVVAAQRPLLD
                     DVEAGRFRADLYARFNGVMVTLPELARRKSDVPSLFLRLLNRRLGAPPPPLDVLLVER
                     LLLYGWPGNVRELELLARRMVGLHGHESRFSLSHLAGTALEMNFVAPAPELSRDERDV
                     QALLSGLRSSHGNLSQAAALARVSRQRAYRLLQSRAEVDLKALRKGSLA"
     gene            6776..8248
                     /locus_tag="VFQ61_23195"
     CDS             6776..8248
                     /locus_tag="VFQ61_23195"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957432.1"
                     /translation="MTGAWAPELLSPRSPNDGSSTPEIEIDSAIPEPNLDGRYELLRR
                     VGRGGMGQVYEARHLRLGRRVAVKLLRAQLGSSDMLRKRFEREARAAAQIEHDRVVAV
                     FDFGVAKNHQPYLVMEYLEGLDLRALLRAEGSLAPPRAVRLLRDACLGLEAAHARSVI
                     HRDIKPENLFVVHPSGGPESCKVLDFGLAKLRAAGASDLHTANGLPLGTLHYMSPEQA
                     RGDGDVDERTDVYGCAAVLYELLSGQRPHTAESPHGLLYKITHEPVRRIEEHDAWLPA
                     GLAEVIHRGLEIERSLRPMSIEAFRKSIEPFLGGSVGAALLSLDGCGGDSPVRVDTEP
                     ELGSPTLVRRRSQFLTTTRRKFAASLVVTACVSSALSSWYTLSRAKGSAPTAAMPACP
                     SAAVESASSASAAPTPALPSTYEGAPAPAAIEPTALAPAAPPRRPRHEAASRRSGPHV
                     PNSVTHASAPEKPGDRAATADTPVPQAPAVEPFDRQNPYE"
     gene            8289..9326
                     /locus_tag="VFQ61_23200"
     CDS             8289..9326
                     /locus_tag="VFQ61_23200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9957433.1"
                     /translation="MSVLFTLCMLLGAPSRAAAEARDEARLHFNRALQLVDGQRFEEA
                     AVEFEQAYELSPNPAVLYNLGQAYAAARHPTQAIAAFRGYLAAASGPEDPRRKQALRE
                     LPRLEALVGVLVLLARPSQVEVHVDGRRVQTTGPLQLDPGRHQIVVSAPGYRAEEREV
                     VAMSGTRETWNLELSPIADQPALEAAQAPRLARAQPHSPPRARNVPLTRHPPTPEDDA
                     VLRRSKQRVWWTATAAGAGLLLGGITAGLVVDNGARYGRWREQQASLDQRWRSAPADS
                     ELIEHQSRNDERAERIKLQDEVAVGLGVAGAACFVGAVMIWMLSDSPPAVSAGLGGSP
                     QAQTGTLSVAW"
BASE COUNT         1705 a         2973 c         2966 g         1720 t
ORIGIN      
        1 ctcttcctcg acgtaactcc atagcgaacg ccctaacgcc gcagccaaac tgcgctcggc
       61 ctcggtggcg tcctcctctg ctttcttgag ggacctctcg gcgacttccc gcgcatcggc
      121 cgcaaccgca cgcgcgcgct cggcggcggc gtgcaggtca gcggccaggt tcaggtcact
      181 gagggccttc tgaagctgag tgcgctgcgt ttcggatcgt tcacccggct tgaagtcacg
      241 cagctgctgc aacaagtgtg cgaccaacga ggcccacaga ttggagtcca cgtagtgcca
      301 agcattgaac tcgacctgga cgatgttggg ccagtatacc ggcgtagccc aaggctctgc
      361 ttgcgcacga gcctgggccg taagctcgtc gatggccaga cgcaggcggc gcatgaagaa
      421 gctcttgccc gaaccccaat tcccgaacac accgagcgag aggctcccat cgagctccgg
      481 agaagcaaaa aggttggcca ccgcagagac ggtcgcatcc agattcaggc aatcatcagc
      541 cgcgcgccca ctcggcacgt ccgcccaata ttccggccgc agggtgcgtg aagcagaggg
      601 ccgtgaagca gagggccgca aagcccgcca gccgtcccgc tcgggctccg ggccgcgcgc
      661 ggcgaggcta tcgacgaagg tttcgtgtac cgttgcacgc tggacgcccg cctgatctag
      721 gattcgatgc gcattgctta ccccaacccg cggtctaaac gtcaagagcg acacgatcac
      781 gtgccgcatt gccagaatcc cgtcgtgatt gggtaagtgc ctcgcggctt ccaagacccc
      841 gtttagattc ggagagagct cgtcgtaacg cggtccgccg ccactcgtac tccttcgctc
      901 tctctcgagg aactcgtcac aggcacttcc gagcgagtgc gtgcccaagc tgtcgatcag
      961 cttcatcagc tcggccgggc cggacgcatc cttgggctga gcgcgacccg tgcgaacgat
     1021 cgcaacgagc aacaggcccg tcccgatctc ccccggcacc cgatactcct gcgcgatggc
     1081 gcgcgccaaa ttgagggctg cccgcagact cgcggataac ctgtgttcgg cgtcttgctc
     1141 gacatcactc accccaacgg acacgtgcgg ttgctgagct tccaattggc cctcttccca
     1201 ggagctttag tgtgccaggg caggcgcggc gcgcccttgg tcattacttc gcaaactcgc
     1261 acttgactca cacgcgcggt cttcgcaccg tatgcggacc atgcccgtag acgctgcatt
     1321 ctgggacaac atcgccgaga agtattctcg taagccgctc gcgaatcccg acgccttcga
     1381 gcgcaagatt gccgtcacca aagcccgaat caagcccacg gacatcataa tcgatctcgg
     1441 ctgcggcacg ggctccttgg cactccggct agccccctgt gcggcccacg tacacggcct
     1501 ggacatttcc agcgagatgg tgcggatcgc gaatgaaaaa gcgctcgctc agaaaatcga
     1561 gaacgtgact ttccacgtcg gcgccttcga cgacacgttc acccgtttcg gacccgagag
     1621 tttggatggg gtgtgcgcct acaatctgct gcatctcgtc gaagatcgag cggccgctct
     1681 ggcgcgcatc catcgactcc tcaagcccgg cgggttcttc atctcctcca acccgtgcat
     1741 gggcgagtcc aagattccct accggcccat tctgcgggtc atgcgctggc tcggcaaagc
     1801 tcccctggtg gagatcttca cgaagcgcac catcgaagac gaaattcgca aagcagggtt
     1861 cgtcgacctc gaccagccgg acgtgggagc caagcccgag atttctttca tcgtcgccgt
     1921 caagccgcgc tgagaaggcg tcggcttctt gcgcccggca ctcgcgagcg aggtcgtccg
     1981 catgagggtg gccccgcctg aatggccatg atgccgccgg tgaacagcct cagctcagcc
     2041 gccgggcgca tcgagcatgc gccggagcag ctcttcgagc tgacgttggt ccgatgcgcc
     2101 gagccggctc gtcagatgcg cgagcgattg tcgtacctga gcgtataggt ccttcaccag
     2161 ggccgcacct tcgctcgtca ggtagacgtc gatggcccgg cggtcttcag ggctgggtga
     2221 gcgcgccacg agtcccctct tttctgctcg atcgatcagg ccggacatcg tggacttctc
     2281 gagacccagg tattcggcca gctctgccat gcggacgcgt cggtcccgca agattccaca
     2341 gactctgagc tgagtgagcg agagatcgtt ctccgcacca atcttgttca gcacggccat
     2401 gcagaagaag gatgtccgca cgagcccgtc caccaatccg tcgctcggca ccgcagcacg
     2461 tctcaccggc ttcgattcaa ccatcgaacg gcagcttgac atagttcgtt gtacgaacca
     2521 aatggttctt gttacgaacc acttgcgggg tcattacccc gctctccaac caagcaggaa
     2581 acccatgaaa gccgctgtca tccggtcatt tggcaagcca ccgagctacg aagacttctc
     2641 cgagcccctc ccgcgaggtc ccgaagagat acgcgtccaa gtactcgccg ctgggctaca
     2701 tcacctcacc cgggcgcgcg ccgacggctc gcactacacg agcgcccaga cgcttccaat
     2761 ggtcccagga gtcgacggcg tcgggcgcgg tcccgacggt aaactgcgct acttcgtggt
     2821 ggatggcggc cagattgggt cgatggcgga ggagacggtc gtcgagatcg atcacagcct
     2881 cgtgctccct cgcgactgcg atcccgtcac cgtggctgcc gcgatgaacc ccgcaatggg
     2941 ctcgtggctt gcgctgcgtt gtcgagtgcc cttcaaaaag cggcagaagg ttctgatcct
     3001 cggcgccacc ggaaatgcgg gcagcatggc agttcaagtc gctcgccatt tgggcgcggc
     3061 gcagattatc gcggtgggtc gcgatcagca gaggctcgcc aaactgggcg cgctcggcgc
     3121 caccgatctc gtgtcgctgg gggacgcacg gctcggcgct ctggcgcgcg aggtcgacgt
     3181 cgtactcgac ttcatttggg gtgagagcag cgcgcagacg ttggagctcc tggtgcgcga
     3241 ccgggcggat cgggcgcggc cgctcacgtg gatcgaggta ggatcggtcg gggggcaaac
     3301 cgccgccatt cccgccagcg ccttgcgcgc ttcacgattg gaaatcttgg gcagtgggat
     3361 cggctcggtt ccgggtcgcg agatcctgaa ggagctgccc gcgctcgtga aggagatcac
     3421 gcgtggcaca ttccgcatcg atgccaaggc cgtcccgctt cgcgccgtgg agcaagcctg
     3481 gtgcgaagcg gcccacatga gcgaacgtgt cgtgttcacg ccgtagtgtc cggggttttg
     3541 cgacgcggag cggcggggtt ccgccagccc ggcgttaata gcccaggcga ggtcgccgaa
     3601 gcctcactcg cacgcctcaa gaccgacgta atcgatctgc tgtatcagca ccggggtaga
     3661 tcccgaagtt ccgatcgagg acgttgcggc acggtcaagg agctaatcca gcaaggcaag
     3721 gtgaaacact ttggaatgtc cgaagccgga gcaaacacgt taagacgcgc ccacgcggtg
     3781 cagccggtca ccgcgttaca gagtgaatac tcgctctggc atcgggttcc ggaggccgaa
     3841 atcctcccgg cttgtgagga gctcgacgtc ggcttcgttc cctttagtcc gctcggcaga
     3901 ggcttcctca ccggcaagct cgaccaaagc tcgtttgggg agaaagactt gcgccgctca
     3961 ctcccgcgct tcacgccgga ggcgatgaag gcaaacgaaa ccttcttaga gctgctgcgt
     4021 gaggtcgccg agcgcaagaa aggctacctc cgctcaggtc gcgctggcct ggttgctcgc
     4081 gcaaaaaccg tggatcgtcc cgatccccgg aaccacgaag ctcccacgtc tggaagagaa
     4141 cctcagtgcc ctccgcatcg agctcacccc agaggacctg cgcgccatcg acacctccgc
     4201 cgcacgactc acggtgcagg gcaaccgcta ccccgacgag ctcgaacgcc tgacgggcct
     4261 ctgaagcgaa acctgtcggc tggctaggac gaaaggcacg gctctctcgc ggttccccga
     4321 gcttcccagc acgccaattc agcggacgtt cgagatcgag tttttggcgg tgtcgtgctt
     4381 ggtcttcatg atgttgctga cggccgtgta ggagcgattc tcatgctgca tctgcgactg
     4441 aagctccaga tattggaggt tgaagctcat ttgagtttct tgcatttctt gcgtagcttc
     4501 gagcaacgcc ccctgtgcgt ccgggccgcc cgaggctgtg tcgacattcg agagacgctg
     4561 cgtcatcgtc tcggtcgcca cctcaggctc ggcctggatg gggtcccctt cggccgcgcc
     4621 gcacgctacg gccaacgaga ccatcaacga tagaatcacc caagagctga atccgccgat
     4681 tccgaattgc gccttcgtcg gttgcggatt cgctgcgaga tctttcaggt tcttcaactt
     4741 accctccacg cgttagccgg aactccaggc tatccgtgcc tgtcgttcgc gaacggttgg
     4801 ttcccgccgc ggcatccatt gtgagcgggg agcgacgccg aggcaagcgg ggtttagttc
     4861 cactcaatcg tagcgatatt gcggcgagac gaccaccaac agcacatctc cgctcacgcc
     4921 gcgcggcccc ccagaatccg tagttgttgt gtccgctgat ggcgcgcggc aagcggcgac
     4981 tcggtcccaa gagatcgatg gcgccggcct cgccatagtt ggaggtgaag atgccggctc
     5041 tcgtccgctc ttccggagtg agtgaggaat acgctctggc aacggcggtc acgatggctt
     5101 cccaaccgtg catgtccgca aacagcggcg ccgctcagcg ccaagcgatt cattcggttc
     5161 caatccatag acgaatctac gctcgaccgg tggcacaatc gggcacacgc caattccggc
     5221 aagccatccc actcagcgac gatggaggtc tgaaacattt cgactgagcg gtggctccgc
     5281 agctgcatgt gcacggcaac tcctgaacgg agtaccgccc ggtcctggag gctttccaga
     5341 ggccgctcga ccgcggccgc tgactcgaga accggatggg actttagcgc gggccgtgtt
     5401 aggtatggcc ttgggtttgt agagaagagg cagcgggtat ggcgaaagat ctcatgagtg
     5461 atagcacttt gccggttttg accggagggg agcaggaggg gcctcccaca ctttggggtt
     5521 tggagttggt gtttccgccg gaagatgcga gacgattccg tatttcctcc gcacgaggcc
     5581 tggccatcgg tcgcgatgca gacgcggacg tggtgattgc cagccatcag gtttcgcgcc
     5641 gtcatgccga tatcgtgtgt gtgggggaag agctctgggt gcatgacagc gggagtttga
     5701 acggaacgca tgtcaatggt gaacgcgtga ttagacgccc ccttcacgag ggagatgtgc
     5761 tccggatcgg ggatcggttg gcggtcgtcg tacgcgatga catcgaggag atgtcgagcg
     5821 caacggtgac gcaaatcgca agcggctttt gggctggttc ggtgctgcaa gcagcgttgg
     5881 cgaacgcgcg taaggccgca cgcacggatc tgccggttgt actgcaagga gcgaccggaa
     5941 caggcaaaga gcgagccgct cgcgcaattc atgaatggag tgggcgggcc ggcgctttcg
     6001 tgcccatcaa ttgtgcggcc attcccgagc acctggcaga ggccgagcta tttgggtacc
     6061 ggaaaggggc atttaccgga gcggataggt ctgccgtcgg tcatttgcgc gccgcgcacg
     6121 gcggcacgct actgttggac gaggtctcgg atctgccact gccactccag gcgaaattgc
     6181 tcagagtggt cgaacaaagc gaggtgcagc cgctcggtga ggcgcgggct ctgccgatcg
     6241 atgtgcggct cgtggtcgcg gcgcagcgcc ctttgctgga tgacgtcgag gccggtcgct
     6301 ttcgtgccga tctttatgct cgttttaacg gtgtgatggt aacgttacca gaattggcgc
     6361 gccgtaagag tgatgttccg agtttgtttc ttcgattgct gaacaggcgg ctcggagcgc
     6421 cgcctcctcc gctcgatgtg ctattggtcg aacggctgct gctctatggg tggccgggca
     6481 atgtgcgcga gctggaattg ctggctcgcc ggatggtcgg gctgcatggg catgagtcgc
     6541 gcttctcgtt gtcccaccta gcggggaccg ctctcgagat gaattttgtg gccccggcgc
     6601 cggagctgtc tcgcgacgag cgggatgtgc aagctctgct ctccgggctg cgcagcagtc
     6661 acggcaacct gagccaagcc gccgcgctag cgcgtgtttc gcggcagcgt gcctataggc
     6721 tactgcagag ccgggccgag gtagacctga aggcgctgcg caaggggtcg ctcgcgtgac
     6781 aggagcgtgg gcaccggagc tcttgtcccc ccgctcaccg aatgacggaa gttcgacgcc
     6841 ggagatcgag atagatagcg ctatcccgga acccaatttg gacgggcgct acgagctctt
     6901 gcggcgcgtg gggcgcggag gcatgggtca agtatacgag gcgcgtcatc tacgtctcgg
     6961 acgccgtgtc gcggtcaagc tgttgagagc tcaactcggc tcgtcggaca tgttgcgcaa
     7021 gcgcttcgaa cgggaggcgc gagccgccgc gcagattgag cacgatcgcg tcgtggcggt
     7081 gttcgacttt ggggtggcca agaatcacca gccctatcta gtgatggagt acctcgaggg
     7141 gctggatctg cgcgcgctgt tgcgtgcaga gggctcgtta gcgccacctc gtgccgtgag
     7201 gctactgcgg gacgcctgtc tggggctcga ggcggcgcac gcgcgaagcg tcatccatcg
     7261 cgatatcaag cctgaaaatc tattcgtggt tcacccctcc ggaggcccgg agtcctgcaa
     7321 ggtgctcgac ttcggtctgg ccaagctgcg cgcggcgggc gcctcggact tgcacaccgc
     7381 caacggtttg ccgctcggaa cactgcatta catgtcaccg gagcaagccc ggggggacgg
     7441 cgacgtcgat gaacggacgg acgtttacgg ctgcgccgcc gtgctctatg agctgctctc
     7501 gggccagcgg ccacacaccg cggagtcgcc gcacgggctc ttgtacaaga tcacccacga
     7561 accagtgcgg cgcatcgagg aacacgacgc ttggctgccc gcaggtctcg ccgaggtgat
     7621 tcatcgcggg ctggagatcg agcgctcgct gcgcccaatg agcattgagg ctttccgtaa
     7681 gtcaatcgag ccctttttgg gaggcagtgt cggcgctgca cttctgtcac tggatggctg
     7741 tggcggggat tctccggttc gggtcgacac cgagcccgag ttgggctcac caacgctcgt
     7801 gcggcgccgt tcccaattcc tgacgaccac tcgccgcaag ttcgcggcat cgctcgtagt
     7861 gacagcatgc gtatccagcg cgctgagctc ctggtacacg ctctcacgtg caaaaggctc
     7921 ggcccctaca gcggccatgc cggcctgtcc gtccgcggcg gtcgaaagcg cgtccagcgc
     7981 gtcggccgcc ccaacgcccg cgttgccctc gacttacgaa ggtgcacctg cacccgcggc
     8041 gatcgaaccc acagcgcttg cgccggcagc gcctccgcgg cgaccccgtc atgaagccgc
     8101 ctcgaggcgg agcggaccgc acgttccaaa ctcggtcact catgcgagcg cacccgaaaa
     8161 gcccggcgac cgtgcggcga cagccgatac accggtacct caggcgccag cggtagagcc
     8221 cttcgaccgc caaaatccct atgagtgaaa cgcatgtttg cgcccgacga ggtgcacgac
     8281 ctcgtctagt gagtgttctg tttaccctct gtatgctcct gggcgcgccc tctcgcgccg
     8341 cggccgaggc ccgcgacgag gcccgactgc atttcaatcg cgcactccaa ttggtagacg
     8401 gtcaacgctt cgaggaggcc gcggtggagt tcgagcaggc gtacgagctg agcccaaacc
     8461 ctgccgtgct atacaacttg ggccaggcgt acgccgccgc gcgccaccct acccaagcca
     8521 tcgcggcatt ccgtggttat ctggcggccg ctagtggtcc cgaagatcca cggcgcaaac
     8581 aagcactccg tgagctacca cggctcgaag cactggttgg tgttctggta ctgctggcca
     8641 gaccgagcca ggtcgaggtg cacgtggacg gtcggcgggt gcaaaccaca ggtccgctgc
     8701 agctcgatcc gggccgccac cagattgtcg tgtcggcgcc gggctatcga gcggaagagc
     8761 gggaggttgt tgcaatgagc gggactcggg agacctggaa tctcgaactc tccccgatcg
     8821 ctgaccagcc ggcattggaa gctgcgcaag caccgcggct cgcccgggcc caaccgcact
     8881 cgccgcctcg cgcgcgcaac gtgccgctca cccgccaccc gccaacgccc gaagacgacg
     8941 cagtgctacg cagatcgaaa cagagagttt ggtggacggc gaccgcggcc ggagcgggcc
     9001 tgctgctagg aggcattacg gccgggctcg tcgtcgacaa cggcgctcgt tatggccgct
     9061 ggcgcgagca acaggccagt ctcgatcaac gttggcgaag cgcccccgcc gactcggagc
     9121 tcatcgagca tcaaagccgg aacgacgagc gtgccgagcg catcaagttg caagacgaag
     9181 tggccgtggg gctcggggtg gcgggcgctg cgtgcttcgt gggagcagtg atgatttgga
     9241 tgctctcgga ctcaccccct gcggtgagcg ccgggctagg tggatcgccg caagcgcaaa
     9301 ccggcacgct ctcggtggca tggtgacgca ctcgaagaac aaagccatga agatacctag
     9361 tttg
//
LOCUS       DASTKU010000666         5178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1914438, whole genome shotgun sequence.
ACCESSION   DASTKU010000666 DASTKU010000000
VERSION     DASTKU010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914438"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..500)
                     /locus_tag="VFQ61_23205"
     CDS             complement(237..500)
                     /locus_tag="VFQ61_23205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957434.1"
                     /translation="MFARLLRWLRPSKPNPVTRLSEAQAIELARRSAAPDSLAEQLTW
                     ARLDTSGVGPRWIVGSSTIGLVLEVVVDDATGEVLEKRTLGVR"
     gene            complement(519..1445)
                     /locus_tag="VFQ61_23210"
     CDS             complement(519..1445)
                     /locus_tag="VFQ61_23210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957435.1"
                     /translation="MSDSVLNWPFEKRLQVTFEKTLPKLGPEARAQLAAIINPTSLAI
                     VAGVLIAWVVSHAFGVGEVIDIIVAVIGIASIGLAVFTGLDYLFDFATGVYRARSERD
                     LDVAADNLAKAIGILGIQAVMAVLFRGAKAPRTGRGGRINLGAAPRNAPLRYKPSIRQ
                     DPTQVAGAGYTTMWGDIVVSSLGSAADRAVVLLHEKVHQFLAPKLYILREYRASNRYA
                     SYTRSSLYRYIEEALAETIAQVGVNGMKQFFSGIRFPVQSGYMYLTKGGGYAAAFAGA
                     GAIPEAASLVYSGTVLGMAFELWFVAGTTPPE"
     gene            complement(1564..2886)
                     /locus_tag="VFQ61_23215"
     CDS             complement(1564..2886)
                     /locus_tag="VFQ61_23215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9957436.1"
                     /translation="MLPLTHLGPGLTLLTGANGSGKSRAAQALARSTAGAQLLSAESQ
                     QAFYEAQLAADESNFKQGVDTSTKVGELLGEAARSHPLFTAFRLEGLWDRGYRLLSTG
                     EARKTLLLQAVLAGPSLLILDEPFDGLDRAAQAELALAILHVAERMAVVCVGTFGARQ
                     LPFPVSALREIVLVDQGQLSFRGSARAFLDRGAAHSQHRAPPIELGTWYPPLDPSVAL
                     VQLRNGCVRYGDFTVFEQLDFSVMPGQHTLIEGPNGSGKSTLLEMITGDHPQAYSNDL
                     QLFGRRRGTGETVWDIKKNVGLVSSRLHRDYRVGGSVESVLLSGLFDSIGVYERVEPS
                     HRARARAWLDWLAIGVGPGAPFRELSFGQQRLVLIARAAIKVPPLVVLDEPTSGLDED
                     NRERVLELAESLCSQRKSTLLLVTHRDDERAFWQNRIGGPILSLGARA"
     gene            complement(2943..4427)
                     /gene="ilvC"
                     /locus_tag="VFQ61_23220"
     CDS             complement(2943..4427)
                     /gene="ilvC"
                     /locus_tag="VFQ61_23220"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF003557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET9957437.1"
                     /translation="MSRQNYFNTLTMAQKLEQLGQCRFMDRSEFNGVEKLRGKKVVIV
                     GCGAQGLNQGLNMRDSGLDVSYALRQDAIDQKRASFTNATSNGFKVGTYEQLIPSADL
                     VCNLAPDKQHADVVSKVMPLMKKGACLAYSHGFNIVEEGQQIRKDLTVVMVAPKCPGT
                     EVREEYKRGFGVPTLIAVHKENDPNGDGLEIAKAYAAATGGDRAGVLESSFVAEVKSD
                     LMGEQTILCGMLQTGSILCFDRMVAQGIDRGYASKLVQFGWETITEALKQGGITLMMD
                     RLSNPAKLKAFALAEELRGILRPLFRKHQDDIITGVFSSTMMKDWDAGDVNLLRWRAA
                     TNDTAFEQTPAGSDKITEQEYFDHGVLMVAMIKAGVELAFECMVEVGMKPESAYYESL
                     HETPLIANLISRKKLYEMNKVISDTAEYGCYLFSHAAVPLLKEFMEKQSTDVIGKAFA
                     PGSQQVDNRSLLAVNQAIENHPVEKVGLTLRGHMTAMKQIAVGG"
BASE COUNT          997 a         1662 c         1563 g          956 t
ORIGIN      
        1 agtccgccta acaacaagca acctccgagt attcgacggg tggttccccg agcagagcgg
       61 gagtactgag cgacatcggc cccaaaccac ctgcatccaa cgagagcgcg tagagctggc
      121 attagctacc gtacgatact caagagtggc caaccctcgg gcttggagct cacattttta
      181 tgtgatttcg aagctggata gcgcctcagg ccagcccggg gatgcgcgac tgtccttcag
      241 cgaacgccca aggtgcgctt ttcgagcacc tcgccggtcg catcgtcgac cacgacttcg
      301 agtacgagtc caatcgtgct cgaacccacg atccatcgcg ggcctactcc ggaggtgtcg
      361 agcctcgccc aggtgagctg ctccgccagt gagtctggcg ccgccgaacg gcgagcaagc
      421 tcaatcgcct gagcctcgga cagtcgggtt accggattgg gcttggaggg cctcaaccaa
      481 cgcaggagtc ttgcaaacat ggcgctttgg aatctcactc attccggcgg cgtcgttccg
      541 gccacgaacc agagctcgaa agccattccc aacaccgtgc ccgagtagac gagtgaggct
      601 gcctccggga tggcgcccgc gcccgcgaag gccgccgcat agccgccgcc cttggtgagg
      661 tacatatacc cgctctgcac agggaagcgg atccccgaga agaactgctt cataccattg
      721 acgccaacct gcgcgatggt ttcggcgagg gcctcctcga tatatcggta aagcgaggag
      781 cgcgtgtacg aagcgtaccg attgctggcc cggtattcgc gaagaatgta gagcttcgga
      841 gccaggaact ggtgcacttt ctcgtgcagt aacacgaccg cgcggtccgc tgctgaaccc
      901 agggacgaga ccacgatgtc accccacatc gtggtgtagc ccgcgcccgc aacttgcgtc
      961 ggatcttgcc gaatcgaagg cttatagcgc aacggcgcgt tgcgcggggc cgcaccgaga
     1021 ttgatgcggc ctccgcgtcc ggtccgcgga gccttggctc cgcgaaatag caccgccatt
     1081 accgcctgaa tgccgagaat gccgatcgct ttggccagat tgtcggccgc cacatccagg
     1141 tcacgctccg aacgcgcgcg gtaaacgccc gtcgcaaagt cgaacagata atcgagcccc
     1201 gtgaacacgg cgaggccgat cgaggcaatg ccgatcacgg ccacgatgat gtcgatgacc
     1261 tcccccactc cgaaggcgtg agacaccacc caagcgatca acactcccgc aacgatcgcg
     1321 agcgaggtcg gattgatgat ggccgcgagc tgggcgcgag cctcggggcc gagcttgggt
     1381 aacgtcttct cgaacgtgac ctgcaatctc ttctcgaaag gccaattaag gacggaatcg
     1441 ctcatgtttg aaccggatcg acggagacgg acggaccaaa gctctcgcgt gcccccatcc
     1501 tacgtcattt gggcacgccc tcttcctcag agcccgcgaa ggtgttcctc cccgcgagtt
     1561 ggctcacgcg cgggcgccca aggacagaat cgggccgcca atgcgatttt gccaaaacgc
     1621 gcgctcatcg tcgcggtggg tcaccaggag caacgtgctt ttgcgctggg aacataagga
     1681 ttcggcgagc tcgaggaccc gctcacggtt atcctcgtcg agcccagagg tgggttcgtc
     1741 cagaacgacg agcggaggga ccttgatcgc ggcccgcgca atcaagacca ggcgctgctg
     1801 accgaaggac agctcacgga acggtgctcc tggccccacc ccgatcgcga gccagtcgag
     1861 ccaggcccgc gcccttgccc ggtggctcgg ctcgactcgc tcgtacacac caatgctgtc
     1921 gaatagaccg ctcagcagca ccgactcgac gctgccgccc acgcgatagt cgcgatgcaa
     1981 tcgagaggac acgagcccca cgttcttttt gatgtcccag accgtttcgc cggtgcctcg
     2041 tcgccgaccg aagagctgaa ggtcgttact gtaggcttgc ggatgatcgc ccgtgatcat
     2101 ctccagtagc gtggacttac ctgaaccgtt ggggccttca atcagcgtgt gttgaccggg
     2161 catcacgctg aaatctagct gttcgaagac ggtgaaatcg ccatagcgca cacagccatt
     2221 gcgcagctgt acgagcgcca cgctgggatc gagcggcggg taccaagtac cgagttcgat
     2281 gggcggggct cggtgttgcg agtgggccgc tccgcgatcg aggaacgcac gagcggaccc
     2341 gcgaaaactc agctggccct gatcgacgag gacgatctcg cgcaaagccg agaccggaaa
     2401 cgggagctgg cgagccccga acgtgcctac gcagactacc gccatgcgct ccgcgacgtg
     2461 caggatggcc agcgccaact ccgcctgcgc agcgcgatcg agaccgtcga acggctcgtc
     2521 caaaatgagc agcgacggcc cggcgagcac ggcttgcagc aacagagtct tgcgcgcctc
     2581 gccggtcgac agtagccgat aaccgcggtc ccagagcccc tcgagccgaa aagccgtgaa
     2641 caacggatgg ctgcgagcgg cttcacccag cagctcaccc accttcgtac tggtatccac
     2701 accctgcttg aagttgctct cgtcagccgc cagctgtgcc tcgtagaagg cttgctgaga
     2761 ttcggctgac aacagctgcg cccccgcggt gctccgcgcg agcgcctgcg cggcgcggga
     2821 tttaccggaa ccgttggcgc ccgtgagcag ggtgagcccg ggtccgaggt gcgtcaatgg
     2881 caacatggat tcggattctc acccctcacg ggtcggtagc tgacggaatt ggccgcgttc
     2941 gttcagccgc ccacggcgat ctgcttcatc gcggtcatgt ggccacgtag cgtgagcccc
     3001 accttctcga ccggatgatt ctcgatggct tgattcacgg cgagcaagct gcggttgtcc
     3061 acttgctggc tgcccggtgc gaacgctttc ccgatcacgt ccgtactctg cttctccatg
     3121 aactccttca ggagcggcac cgcggcgtgg ctgaatagat aacatccgta ttcggccgta
     3181 tcgctgatca ccttattcat ctcgtacagc ttcttgcgcg agatcagatt ggcaatcagg
     3241 ggcgtctcgt gcagcgactc gtagtaggcc gactcgggct tcatgcccac ctcgaccata
     3301 cactcgaacg ccagctccac ccccgccttg atcatggcga ccatcagcac gccgtgatcg
     3361 aagtactcct gctcggtgat cttgtccgag ccggcagggg tctgctcgaa agcggtgtcg
     3421 ttggtggcgg cgcgccagcg aagcaagttt acgtctcccg cgtcccaatc cttcatcatc
     3481 gtcgagctga aaacacccgt gatgatgtcg tcttggtgct tacggaacag cgggcgcaag
     3541 atgccgcgca gctcttccgc cagcgcgaat gccttgagct tcgcgggatt gctcaagcga
     3601 tccatcatca gcgtgatgcc accctgcttg agcgcctcgg tgatggtttc ccagccgaac
     3661 tgcaccagct tgctggcgta gccacggtcg ataccttggg ccaccatgcg atcgaagcag
     3721 aggatcgagc ccgtctgcag catgccgcag agaatcgtct gctcgcccat caaatcactc
     3781 ttcacctcgg ccacgaagct cgactcgagc acgccggcgc ggtcaccacc ggtggcggcg
     3841 gcgtaggcct tggcgatctc gaggccgtca ccgttcggat cattctcctt gtgcactgcg
     3901 atgagcgtgg gcaccccgaa accgcgcttg tactcttcac gcacctcggt gccggggcac
     3961 ttcggggcca ccatgactac cgtcaagtct ttgcggatct gctgcccttc ctcgacgatg
     4021 ttgaagccat ggctataggc caggcaggcg cccttcttca tgagcggcat caccttcgac
     4081 accacgtctg cgtgctgctt atccggcgcc aagttgcaca ccagatccgc gctcgggatc
     4141 agctgctcgt acgtgcccac cttgaagcca ttgctcgtgg cgttcgtgaa gctcgcgcgc
     4201 ttttgatcga tggcatcttg tcgcagcgcg tagctgacat cgagccccga gtctcgcatg
     4261 tttaggcctt gattcagccc ctgcgcgccg cagccgacga ttacgacctt cttgccccgc
     4321 agcttctcga cgccgttgaa ctcgctccgg tccatgaaac gacattggcc gagctgctcg
     4381 agcttctgcg ccatcgtgag agtattgaag tagttttgac gggacatgtg cgctcacagt
     4441 tctgatgcct gggcggcatc tgctggagcg gaggatagga tccccaaggc cctcgggcaa
     4501 cgttgcaccc gcgcctattt tcggcaggcc ttattgcgaa aaacgcaaca tagctccgac
     4561 cgaaatacag gctcggctcg caaggcgccc attcaggcag taaacccgct atctgggggc
     4621 aaacgtcgga gcaaacgtcg gaaccacctt cgttaagaac gaccttcgtt ccaggccgct
     4681 tctgtcggcg cggatgccgt gacattgccg caaaacggct gtggcaagga tcggatactc
     4741 gcccacgtac acgtcggcac gcttcactag ttcagtggca gcgacgggtg aagcgggtca
     4801 cggccggggc cacgtcgact tggggggccc gcgcggtatt acgggagcaa gctggagtga
     4861 gttttctgcg ccgacggggc tcggggacgg gcggcgcgca cgtttacaga acggccgagg
     4921 aaagaatcag tctccccctc ggatgcgcgt cggtgtgagc aaccgaccac gtgtgctgag
     4981 ggtgcctatc ggttagcgac cgcacacaaa tatctagtgt cagttggtcg ccccggactg
     5041 tctgcaatat cgtacgccga atgacgcaag ctccatgaac ccggtcaggg gtaaggggcg
     5101 gcggggttag gggccgaggg ggagctcgac cgtggcgagc gaaccctcca gcgccggagg
     5161 cgggccgttg tgttgcgg
//
LOCUS       DASTKU010000667         4368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1690463, whole genome shotgun sequence.
ACCESSION   DASTKU010000667 DASTKU010000000
VERSION     DASTKU010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690463"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..869)
                     /locus_tag="VFQ61_23225"
     CDS             complement(108..869)
                     /locus_tag="VFQ61_23225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957438.1"
                     /translation="MGAQRHLHLVHSIDRGEIEIGTALARRFLGMAEDEPTELTFLVR
                     GRTHVAHAMNAREHVRLLREAEQIKGFNGAYQLVNGPINPALLARYAPNRIHRAWNGR
                     VGEGDIRQRRAIYIDIDPVRPKGIGATHAEKAAAFEVCEAVEAHLETHLGDACALGHG
                     DSGNGYFILIALQPVETSVAITAGIQQLLRLLAARFNTPHARIDVSVANVARLMPAPG
                     TWKCKGENTPERPYRRTAFSCRAPVRRVSLETILG"
     gene            complement(1037..1684)
                     /locus_tag="VFQ61_23230"
     CDS             complement(1037..1684)
                     /locus_tag="VFQ61_23230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957439.1"
                     /translation="MATQPKPAPWGDINDFRDELFEWLVREGYSPARSSPAKAALLVQ
                     KEGAELPALATTVAQALYRVECFRGTALSIEGSLSPRLKEELTPEQYSVFIDRRREIE
                     AERRVRKLQTDLELIKRDIVALAKRSEKRLELQLCAPHIMKPSLARVRVRRKLALYAL
                     GAAREDALTLHTAVCLLAPKSTRYAPTLAGWRKLEKELRATLAKADFPMHLLPKE"
     gene            complement(2177..2632)
                     /locus_tag="VFQ61_23235"
     CDS             complement(2177..2632)
                     /locus_tag="VFQ61_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957440.1"
                     /translation="MRELIGDRFGLLQGSASHSVAPRRLSTLVVASEIQYGTQLAELV
                     RLLGHEARFTTSVREAIELLDVRMPQLVFTELALPDGTASQLANAVHARTTFLCGMAA
                     LLKKELGLVELGALWRAGFDAVLSSPCDKSSLAAMLDWFQARLPVAPVN"
     gene            complement(2842..3378)
                     /locus_tag="VFQ61_23240"
     CDS             complement(2842..3378)
                     /locus_tag="VFQ61_23240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957441.1"
                     /translation="MENLDALTKRALRAYRLGRLRMAARVTLLLLPVATLCLIEPAGR
                     EACACCAALLVTAAVWFRFRDRKGADAVTTGLLAGAVPLASGMILGAMDLGCATATSP
                     SLCSGLSLVSGVLAGIFITLRESNNHGRVRSGWLPLLVAVLVGSMGHARLGATSIVAT
                     ALGLIMGSLLARQARRFA"
     gene            complement(3365..3904)
                     /locus_tag="VFQ61_23245"
     CDS             complement(3365..3904)
                     /locus_tag="VFQ61_23245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9957442.1"
                     /translation="MSPERRQAIQDAVLRLADGDRSAMPGLVPELWPLLLSFAQRGLR
                     NAHDAEDVAQEVFLRICARISEFDRSRDALSWAFGIASFEIMTHRRRLQRRRESFSVS
                     GMPEDSHTSPSAEDNAIEAELRVALVEALGELTPTELAQLGFGNSHPAGPSTPAMRKR
                     RQRTLERLRGIWRKVYGEP"
     gene            4033..4338
                     /locus_tag="VFQ61_23250"
     CDS             4033..4338
                     /locus_tag="VFQ61_23250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957443.1"
                     /translation="MTSPHPTSAPVLPKRSFGIAGLAGFAACVACCALPMFAAAGVGG
                     GLLSTVAGYIQPGADLVIGGLVGAGVLSFLTIRSRRLKREKSCGAACSTEGGCGCRR"
BASE COUNT          842 a         1388 c         1369 g          769 t
ORIGIN      
        1 cgttcgcgag cctgaagacg ctctcttcgc gctgcccacc gccagcggct tctggcggct
       61 tctcggctgc gccctcagaa gggtgaacct gcagagtgtg aatgaggcta gccaaggatc
      121 gtctccaatg aaacgcgccg aacgggagcg cggcaggaaa aggccgtgcg gcgatagggc
      181 cgctcgggag tgttctcgcc cttgcacttc cacgttcccg gcgcgggcat cagccgggcg
      241 acgttggcga cggacacatc gatacgcgcg tgcggcgtgt tgaatcgcgc agcgagcaac
      301 cggagaagtt gttgaatacc ggccgtgatc gcgacgctcg tctcgaccgg ctgcagagct
      361 attaggatga aatagccatt gccgctgtca ccgtggccta gcgcgcaagc gtcccccaga
      421 tgagtttcga ggtgcgcttc taccgcctcg cagacttcga atgctgccgc tttctccgcg
      481 tgggtcgcgc cgatgccctt gggtcgcacc gggtcgatat cgatgtagat ggcccggcgt
      541 tgacggatat ccccttcacc gactcggccg ttccaggccc gatgaatccg attcggcgcg
      601 tagcgcgcga gcagagcggg attgatcggc ccgttcacga gctgatacgc cccgttgaag
      661 cccttgattt gctccgcctc gcgcagcagg cgcacgtgtt cgcgcgcgtt catggcgtgc
      721 gcgacgtggg tgcggccccg caccaggaac gtgagctcgg tgggctcatc ctccgccatc
      781 ccgagaaagc gccgagcgag cgcggtgccg atctcgattt cgccccggtc gatggagtgg
      841 accagatgca ggtgtctttg cgcgcccatc agccactccc cggggcgcgc gaactccgcc
      901 cgagggaacg tcgcgcgtcg ttgatatctg caagcatgcg gttcaccacc tgctcacagt
      961 cttgggtcgg ggccctaaaa tggccagggc cgccccccgc gggaccttga ctcactcagt
     1021 agagtgtgat cgggctctat tccttgggca aaagatgcat cgggaaatcc gctttagcga
     1081 gcgtggctcg gagctccttc tccaacttgc gccaccctgc cagggttggc gcgtagcgcg
     1141 tgctcttcgg agccagcagg cagaccgctg tatgaagcgt gagggcatcc tcacgggccg
     1201 ccccgagcgc gtatagcgcg agctttcgcc gcacccgtac cctcgccaaa ctgggcttca
     1261 tgatgtgcgg cgcgcacagc tggagctcaa ggcgcttctc gcttcgcttg gccaaagcca
     1321 caatatcgcg cttgatgagc tccaagtccg tctgcagctt ccgaacccgg cgctcggcct
     1381 caatttcgcg gcggcggtcg ataaacacgg agtattgctc gggggtgagc tcctccttga
     1441 gccgtgggga cagagagccc tcaatactga gcgccgtccc gcgaaaacac tcgacccggt
     1501 agagcgcctg cgcgacggtt gtagccaggg cgggcaactc ggcaccctcc ttctggacca
     1561 gcagtgcggc cttggcgggt gagctgcgcg cgggcgagta gccctccctc acgagccact
     1621 cgaacagctc atctcgaaaa tcgttgatgt cgccccaggg tgcgggcttc ggctgcgtcg
     1681 ccatgtctgc ggggtgagat tggcacgctt cccttccgtc agtaacgcaa gcaccgacga
     1741 cacttcgggg gggtgtcagc ctcgtgcttg ccatgacacg tgccagagct tggcaacctc
     1801 tgcgtgcacg ctgcagttgg agcagggctg ggccgaaaac agcctcaaaa ccatcagagc
     1861 aacgaagcgt gccgcgcctg acacccgggg atggcaccct gcagcggcaa ctatagccga
     1921 ggcagttgtc caatcgttcg tcgccataac gtttgcccgt gaacggccaa cgagagccgc
     1981 ggaaatttaa cctttggtcg gtcgcccaag cgcgctttgg ttcggatagc cccatgcgag
     2041 gagcggcggc aaacgactgg aggcgcagcc ggcgagaact ggggtcggag ctgtgaacgg
     2101 ccaacaggat ccgcgtaaat gtgaccacgt cgtcggtcga ccggcgaacg cgcccgtaat
     2161 cgcgctcccg ttcagactaa ttcacgggcg caacgggcag tcgagcctgg aaccaatcga
     2221 gcattgctgc aagggacgac ttgtcacagg gacttgagag caccgcatca aatcccgccc
     2281 tccagagggc tccaagctct acaaggccga gttccttctt cagcaaagcc gccatcccac
     2341 agagaaaggt agttcgggca tgcacggcgt tcgcgagttg gctcgcggta ccgtcgggga
     2401 gcgcgagctc cgtaaacacc aactgcggca tcctcacgtc gagaagctcg atggcttctc
     2461 gaaccgaggt agtgaagcgt gcctcatggc ccaacaaccg cactagctcc gcgagctgag
     2521 taccgtattg aatctcgctc gcgacaacga gcgtactcaa cctgcgcggt gcaaccgagt
     2581 gagaagcgct cccttgtagc agcccgaatc gatcgccgat cagctccctc atgcacgttc
     2641 tttttgggtc gaaactcaaa aaattgcaag acggtccgag ccaacacagc tctcgcgagc
     2701 gaagcggcaa aaggcaacgg ggcaaacacg ctccagggct cggcaatctg atgtcgagaa
     2761 tccacgagcc tcccgctccg ggcaaacttg gacaagcgtc aaagccgaaa cgacgaagca
     2821 gtcggatcac attctctcgc gtcaagcgaa gcgacgtgcc tgccgcgcga gtaagctgcc
     2881 catgatgaga cctagtgcgg tagccacgat gctcgtagcg cccagtcgcg cgtgccccat
     2941 actgcccaca agcacagcaa cgaggagggg gagccatccg cttctcacgc gaccatgatt
     3001 gttggattcg cgaagggtga taaagatccc ggcaaggacg ccgctcacga gcgacaagcc
     3061 tgagcaaagt gagggggagg ttgctgttgc gcagccaagg tccatggcgc ctaaaatcat
     3121 cccggatgcg agcggcactg ctccagccag taaccccgtg gttacggcat ccgctccctt
     3181 acggtcgcgg aagcggaacc aaacggcagc ggtaacgagc agcgcggcac agcaggcgca
     3241 agcttctcgt ccagcgggct cgatcaagca aagcgtcgct accggtagca gaagcagggt
     3301 cacgcgcgcc gccatgcgca agcggcccaa tcggtaggcg cggagcgcgc gtttcgtcag
     3361 tgcgtcaagg ttctccatag accttcctcc agatgccgcg caggcgctcg agtgtgcgct
     3421 gacgacgctt gcgcatggcc ggtgtcgagg ggccggcagg atgcgagttc ccgaagccca
     3481 gctgagccag ctctgtcggc gtcagctcgc ccaatgcttc caccagcgca acgcgcagct
     3541 cggcttcaat ggcgttgtct tcagccgacg gcgaggtgtg cgaatcctcg ggcatcccgg
     3601 aaacgctgaa cgactcgcgc cgccgctgaa gacggcgacg gtgggtcatg atttcaaagg
     3661 aggcgattcc aaacgcccac gacaaggcat ccctagaccg atcgaactcc gatatgcgcg
     3721 cgcagatgcg gaggaaaacc tcctgcgcca cgtcctccgc gtcgtgtgca tttcgcaatc
     3781 cgcgttgggc aaacgatagg agcaacggcc acagctccgg aaccagccct ggcatcgccg
     3841 atcgatcccc atcggccaac ctaaggacgg cgtcttggat ggcttgtctt cgttcgggac
     3901 tcatcaggtg gacggactcg cagcactcgg agtaggcgcg gcgcccgaga ccgtgacgca
     3961 ctttttgaac gaggccacgt cacgacgggg ccggaccggc ctagtgccgg tgaacattag
     4021 caggagaagc acatgacatc gccccatcct acttccgcac cagttctccc caaacgcagc
     4081 tttggcattg ccgggctggc cggctttgcg gcttgcgtcg cgtgctgcgc cctcccgatg
     4141 tttgctgcgg ccggcgttgg aggcggattg ttatcaacgg tcgcgggcta catccagcca
     4201 ggggcagatc tcgtcatcgg cggtctggtc ggtgctgggg tactcagctt cttgaccatt
     4261 cggtctcgaa ggctgaagcg cgagaagtct tgcggagccg cgtgttcaac ggagggtggc
     4321 tgcggctgtc ggcgttgact caacgagctt cgtgcgcgcg cggaactc
//
LOCUS       DASTKU010000668         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1914805, whole genome shotgun sequence.
ACCESSION   DASTKU010000668 DASTKU010000000
VERSION     DASTKU010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1914805"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VFQ61_23255"
     CDS             complement(<1..408)
                     /locus_tag="VFQ61_23255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9957444.1"
                     /translation="MSSPHNFSGGPGALPQVVLEQASRALIQAPGSPLGLLGISHRSD
                     WFREAVAEAERNTRQRLGLSDDFAVLFLQGGGSLQFTMVPMLLLRGREQPADYIESGY
                     WSGKAIPDARLEGPVRVAWSGRSAGFARLPTEDE"
     gene            complement(405..>3567)
                     /locus_tag="VFQ61_23260"
     CDS             complement(405..>3567)
                     /locus_tag="VFQ61_23260"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HET9957445.1"
                     /translation="PPVPGQPTRELVSSGVPARDIEVAIVDVVSAERLGDRRVGEIWV
                     RGPNVASGYFADPEQTQDTFGGVLGDGEPWLRTGDLGATLDGQLFVTGRLKDLIIVRG
                     RNLYPQDVERTVERACSLVRPGGVVAFSGLNEGLYIIAEVRDPVDGQGTRQSAPFESA
                     LDQLVRAVVEEHELAPTRIVLVPARSVRKTSSGKLARRATRADFEQGALQVLAQYPAS
                     NLAQCSSPYRQLEHVFRRLDARHTRYQFDLELDVPWAQLDAPGDYYGPEALRGYGYDV
                     DALRASAGAWRLFQWRTALSIATGFEELERALISFTRRESAHFSGVKSVPVLEQEEVK
                     HVALFKRYAEHLAAQRPEHAAHVTEKGSELRRELEAFAHPEIFPSVAAYHYSWWLVIL
                     FFEELTIWLYEVLAAEQGIQPAWLAAHRLHAREEAQHVLTDFAFLQSIEASDEERLAW
                     SEQVFQRVAPRLALAMSPEAGLELTLAAYPDLGVRQIRRNPGSPFAALLRNSSFSRTC
                     AAGPYFARVARGDAHPTQDAKPLPSSEDARDLEAWILRWVTENVSLPAGGARVDDVHR
                     DLQAVGLDSVQAVALSGALERHLECRMPLSCVYDHPTAARLARFALARASAGVTAVQS
                     EPEPLLARQRRLLALEVAQPGRVLDHISLLFESSTGDAESWLRALAELVQRQPQLWTQ
                     FAGPPSFEPGLCSDLGSPPRLDELPVIVVQRPFNDARLAQRLAELDRTPFILKGAPPW
                     RAELWAQERRTALLLTMHHVCADGWSTEVLFRELDALGAAVPRAVPRIPAVLAAPGLA
                     REQSLLRSAEAVRRLEFARALLKGHPDLERPGRRTRGQVRAAGASLTATQWTMLAELA
                     RRHQASPYLVLLTVFHQAWRTHTRRDDLLIQTHLFNRVSEQERQSVAYLVNLLVLRTE
                     IHDGMAFGAALERVRGAWLEATEQELPIDHLVRELFPERYAEPWMPARVAFNMLLPPS
                     ESGQVLTWRHELAPTPSFLFFDGMLLGRPRAQGLDLTFWYDGEAFAPEQAEGFMQSFG
                     TRLRSLLEAGVSTQSPARPPEVP"
BASE COUNT          691 a         1212 c         1078 g          586 t
ORIGIN      
        1 ctcgtcctcc gtcggcaagc gagcgaaacc cgcgctgcgg ccactccaag ccacgcgcac
       61 aggcccctcc aaccgcgcgt ccggaatcgc cttcccactc caatagccac tctcgatgta
      121 gtcggcaggt tgttccctcc cgcgaagcaa aagcatcgga accatcgtga actggagtga
      181 cccaccacct tgcaggaaca acacggcgaa gtcgtccgat agccccaggc gctgccgtgt
      241 gttccgttcg gcctcggcca cagcctcacg aaaccagtcc gagcgatggc tgatcccgag
      301 tagcccgagc ggtgaccccg gggcttggat cagagctcga ctggcctgct ccaggacaac
      361 ttgagggagc gcaccagggc caccggaaaa gttgtgcggc gagctcacgg tacctccggt
      421 ggacgtgcgg ggctctgggt cgaaactccg gcctcgagca acgagcgcag gcgtgtcccg
      481 aagctttgca tgaagccctc cgcctgttcc ggggcgaagg cttctccgtc gtaccaaaag
      541 gtcaggtcga gaccttgcgc gcggggccgc cccaacagca tgccatcgaa aaacagaaaa
      601 ctgggcgtcg gcgccagttc atgccgccag gtcaaaactt gaccgctctc gctgggcggc
      661 aaaagcatgt tgaaagcgac gcgggccggc atccacggtt ctgcgtagcg ttcgggaaac
      721 aactctctca ccaggtggtc aatcggcagc tcttgctcgg ttgcctcgag ccaagcgccc
      781 cgaacacgct cgagtgcggc gccgaaggcc attccatcgt ggatctccgt gcgtaggacc
      841 aacaagttga ccagataagc gacggactgc cgctcttgct cggacacgcg attgaacagg
      901 tgagtttgaa tcaaaaggtc atctcggcga gtgtgcgtac gccacgcttg gtggaacacg
      961 gtcaagagca cgagataggg gctggcttgg tgacgccgag cgagctccgc cagcatcgtc
     1021 cattgcgtgg cggttaggct tgccccggca gcgcgcactt gcccacgagt gcgccgcccg
     1081 gggcgctcga gatctggatg gcctttcagc agagcgcgag caaactccag ccgtctaacc
     1141 gcttctgccg agcgcaggag agactgttcc cgcgccaggc ctggcgcggc gagcacggcg
     1201 ggaatgcgag gaaccgctcg tggcacagcc gctccgagag cgtccaattc ccgaaatagt
     1261 acctctgtcg accaaccatc cgcgcacacg tggtgcatcg tgaggagcag cgccgttcgg
     1321 cgctcttgcg cccaaagctc tgcgcgccag ggcggggccc ccttcaggat gaaaggtgtt
     1381 cggtccagct cggcgagcct ttgtgcgagt cgagcatcgt tgaacggtct ttgcaccacg
     1441 atcaccggaa gctcgtccaa gcgaggcgga gaccccaaat ccgagcagag tcccggttcg
     1501 aacgaaggcg gccccgcaaa ttgcgtccag agctgcggct gccgttgcac cagctcggcg
     1561 agcgcgcgca gccacgattc cgcgtctccg gtggagctct cgaacaacaa gctgatatgg
     1621 tcgagcactc gacccggttg cgccacctcg agcgccaaca atctacgctg ccgagccagc
     1681 agcggctcgg gctcgctctg caccgcggtc acgcccgcgg aagcacgcgc caacgcgaac
     1741 cgcgcgagcc gcgccgccgt cgggtgatcg tacacacacg agagcggcat gcggcactcg
     1801 aggtgccgct ccagggctcc cgagagcgcg accgcttgga ccgagtcgag accgacggcc
     1861 tgcagatctc ggtgtacgtc atcgacgcga gcgcctcccg cgggcagcga gacgttctcg
     1921 gtcacccatc gcaagatcca ggcctccaaa tccctggcgt cttcactcga cggcaacggc
     1981 tttgcatctt gggtcgggtg ggcgtcccct cgggcgacgc gggcgaagta gggccccgcc
     2041 gcgcaagttc gtgagaagct cgagttcctc aacagtgcgg cgaacggaga gcctgggttc
     2101 cgccggatct gacgcacccc gaggtccgga tatgcggcga gcgtcaactc cagccccgcc
     2161 tctggactca tggccagcgc gagcctcggc gccacccgct ggaaaacctg ctcggaccag
     2221 gccagccgct cttcatcact tgcctcaatc gactgcagaa aagcaaagtc cgtcaggacg
     2281 tgctgtgctt cttcccgagc atgcagacga tgggccgcca accaggcggg ttgaatcccc
     2341 tgctccgccg ccaaaacttc gtataaccag atcgtcagtt cctcgaagaa taggataacg
     2401 agccaccaac tatagtggta ggcggcgaca ctcgggaaga tctccgggtg ggcgaaagcc
     2461 tcgagttcac gccgcagctc tgaccctttc tccgtaacgt gcgccgcatg ctcagggcgc
     2521 tgggccgcca agtgctccgc gtaacgtttg aacagagcga cgtgtttgac ctcctcttgc
     2581 tcgagcaccg ggacactctt cacgcctgaa aagtgagcag attcacgccg cgtgaaagaa
     2641 atcaaggctc gctccagctc ctcgaaacca gttgcaatcg agagcgccgt acgccactgg
     2701 aacagccgcc aggcacccgc gcttgcccgc agagcgtcca catcgtagcc gtacccgcgg
     2761 agcgcctcgg ggccgtagta gtcaccaggc gcatcgagtt gcgcccaggg tacgtcgagt
     2821 tccaagtcga attgatagcg ggtatggcgc gcgtccaagc gccgaaacac atgctcgagc
     2881 tgccggtacg ggctcgagca ctgcgccagg ttcgaagccg gatattgagc cagcacctgg
     2941 agcgctcctt gctcgaaatc cgcgcgcgta gcgcgacgcg ctagtttgcc gctcgaggtc
     3001 ttcctcacgc tgcgcgcggg caccagcacg atccgcgtcg gcgcgagctc gtgctcttcc
     3061 accaccgcgc gcacgagctg atccagtgcg ctctcgaacg gcgctgattg ccgagttcct
     3121 tgcccgtcca ccgggtcccg aacttcggca atgatgtaga gcccctcgtt cagtccggag
     3181 aaggccacta ctccgccggg ccgaaccaat gaacacgccc gctcgaccgt tcgctcgaca
     3241 tcttggggat acaggttgcg cccgcgtacg atgattaagt ctttcagacg ccccgtcacg
     3301 aacagctgcc catccagggt tgccccgaga tcaccggttc ggagccatgg ttcgccgtca
     3361 cccaagaccc cgccgaaggt gtcttgggtc tgctcgggat ccgcgaagta gccgctggcg
     3421 acgtttggac ctcgcaccca aatttcaccc acacgtctat cccccaagcg ttccgcgctg
     3481 acgacgtcca caatcgcaac ctcgatatcc cgcgctggaa cgccagaaga aacgagctcc
     3541 cgcgtaggct gccccggcac tgggggc
//
LOCUS       DASTKU010000669        21590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1915166, whole genome shotgun sequence.
ACCESSION   DASTKU010000669 DASTKU010000000
VERSION     DASTKU010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 21590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21590
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1915166"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..1757)
                     /locus_tag="VFQ61_23265"
     CDS             complement(249..1757)
                     /locus_tag="VFQ61_23265"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9957446.1"
                     /translation="MNEAHIDAARVNAGTGDSGLGKSGSRGTGAAAASVVAEPAPPVT
                     GEPFAPLPALSGVRSILRYRGRPLLRLEHARAASEHATAFEVLGDRFLVLFDPKAVEQ
                     VLVTQHAAFQKDRFTKELRRILGTGLLTSEGEEWRRSRKLMASSFQPNEIAAYGGIMA
                     ERAREFVVTRRAGEVFDVHSSMMHLTLDILVRALFGMEVKQAKEVEGLLAPFVDDYRP
                     GSLAWRLVPEWVPLPSRRRLARTRRELDVILDSLIAERRQQQSSAEQQRSGEQASQSA
                     DLLGRLMLARDVAGSLSEVALRDEAMTLFLAGHETTALALTYALRLLALHPSEARVAR
                     AEIASVLGERLPTMQDVPRLVRIRAALDESLRLYPPVWGMAREPKEDVVISGVLVPKG
                     VHVVVCPWVMHHDARFFNAPERFQPSRWIDQPSPPRFAYLPFGAGPRVCIGSHFALAE
                     AVLVLATILQHVELELLPEPALELVPSVTLRPRGPVPMRFWPRAAPSVARRD"
     gene            2017..4254
                     /gene="tssI"
                     /locus_tag="VFQ61_23270"
     CDS             2017..4254
                     /gene="tssI"
                     /locus_tag="VFQ61_23270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HET9957447.1"
                     /translation="MAKTRHATVVTPLPQELLLLSFEAREQLAQPFEYQLELLAESDD
                     VDLSKLLGESVRVELELADGKTREFTGFVTHASALGQEGRYFKYRAVMRPWLWFLTQS
                     WDCRIFQKETIPEVVKSVFRQHGFTDFETELTGQYRSLEYIVQYRESDFNFVQRLLEQ
                     EGVYYYFRHEKGRHKLVLCDSISAHAPKPGYESIPYYPPQPGEHRERDSLDAWLVSRQ
                     LQSGSLELRDFDFEKPRAELGYRSETPLGHAHDYYPRYDYPGVFSNRAEAETQTRVRL
                     QAIQAGFELVRATGNARAMCTGYLFSLEKYPREDQNKQYLILETNYEIHVNDYETTGN
                     NSDRPECRVELVALDAKRVFRPERRARKPIVEGPQTAIVVGYSGQEIWTDKYGRVKVQ
                     FHWDRAGKSNETSSCWVRVSQLWAGSGFGGIHIPRIGQEVIVDFLEGDPDRPIITGRV
                     YNADNMPPYDLPRNQTQSGIKSRSSKGGGAENCNEIRFEDLKGSEELFIQAEKDQNTN
                     VKNNQSITIGVNQTETVGANQTLTVKANQTITTLANRTVTVLANEAIQVSGTRSTNVT
                     LADTQTYAATRTVQVAQADTLTVNGGRNVAVTGADTTTVSQGGKTTSVPDGAYGIDAK
                     SYGVDSQSSLSLTCPSASIQAGPPAPGAPSGGPPTALTLIAQDALILQCGENVIYIGK
                     DGISISSPSSVTALVGDNALDLAKDCATLTGKNATVTSSGSTDVMGGQVNLRKAPPKK
                     VIKKV"
     gene            4290..5309
                     /locus_tag="VFQ61_23275"
     CDS             4290..5309
                     /locus_tag="VFQ61_23275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957448.1"
                     /translation="MDRDVVTIGLRARSLAAAYTLSALAGLWGCAADTNRHALGLASP
                     SAANDRPADSGESAAASAPPVRHDSATERAALDACYAASSAQHPGLSVNSTALYLVRG
                     GKYIYVDFELAEAPELARCIRESMLQWQAFQPPGSDPGAVGVGGRPIRLGPAPTERPA
                     AETFAAHRERWRRVVNSALEKGILDRNDPIVQETLHPKPEWPSQAQRAQLESCYANHV
                     STKPTPELHLEAIYLARGARVLLADVLLPDPKAPRAPEFAACLAGRIQSWPAPFEGTR
                     ETVFSSFFIHLGTNKEEFPNLPASPELLLARRRGLILRAADLRLVSQDDPVWALLNQQ
                     PGLRK"
     gene            5570..5779
                     /locus_tag="VFQ61_23280"
     CDS             5570..5779
                     /locus_tag="VFQ61_23280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET9957449.1"
                     /translation="MKLSARNVISGKVIAITKGVTTAHIKLEIAPGSVITASITNEAV
                     DDLGLAVGREATAIIKSSDVIIGVT"
     gene            complement(5914..6354)
                     /gene="tsaA"
                     /locus_tag="VFQ61_23285"
     CDS             complement(5914..6354)
                     /gene="tsaA"
                     /locus_tag="VFQ61_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HET9957450.1"
                     /translation="MAESFDPSVSGLFLLRPIGTIRSQLTARRDAPKQGSEGAPDAWL
                     EIEPWAIDAVDGLAVGDEILLITWFHLAQRDILKVHPRSDPQNAIRGVFATRSPDRPN
                     PLGLHPVHVRSIEHGRLRIGPIEAIDGTPVVDIKPVLACEPRLG"
     gene            6445..8748
                     /locus_tag="VFQ61_23290"
     CDS             6445..8748
                     /locus_tag="VFQ61_23290"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HET9957451.1"
                     /translation="MDIKKTIAALTLEEKVALCSGQDFWRTKAIERLGIVSYMLTDGP
                     HGLRKQTSEAEVAALHDSVPATCFPSGAGLAATWNRSLLEEVGEALGREARAEDVGVL
                     LGPAINIKRSPLCGRNFEYFSEDPYLAGELAKYHVRGVQAQGVAACPKHFAANNQEKY
                     RMLVDARVDARSLREIYLPAFESTVKDARPWTLMCAYNRLNGVYCAQSAWLLNQVLRD
                     EWGYDGVVVSDWGAVDDRVESLRAGLELEMPGNGGITDRELADAVRAGKLDERVLDTA
                     LERLLTLYSRVQDGRQPGTPFDREGHHALARRVAQESLVLLKNSNELLPLPQAGRIAF
                     IGTFAAEPRYQGGGSSHIQPTRVENALDAARELLAGSADVVFARGYDVRSSAPDPTLI
                     GEAVELARGADAAVLFVGLTDTFESEGFDRAHLRIPESHVALIDAVAAVQPRTVVVLS
                     NGSPIEMPWLPRVGAVLEGYLGGQAGGGAAADILFGEANPSGKLPETFPVRIQDTPSY
                     LNFPGEKDRVEYREGLFVGYRHADSAEVEPLFPFGFGLSYTTFAYSEPHVDREELQEH
                     EELTVSVRVKNTGKRAGQEVVQLYVRDEVSSVVRPYKELRAFEKVALEPGEEKEVRFN
                     LGKRAFAFWDDRQHDWRVEPGEFTIFVGASSRDLRGRVKVTIRNATQPVPHWDQNSLL
                     GDVMAHPVVGPAITALKNEFSKALGQYPPDSAEALMAEAVAREMPLRNVVRMLRGLNQ
                     EQLSLLLDVLNGATPPERLLSELPTHR"
     gene            8848..9516
                     /locus_tag="VFQ61_23295"
     CDS             8848..9516
                     /locus_tag="VFQ61_23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HET9957452.1"
                     /translation="MLLVHPHPLLALGAIQVEYSSPLSEQPDLAWLSELLKPGAATPL
                     APVTDRLRSSVRDLLRHGGYKPTGRGKPASEYLVRAADEGSLKPINPAVDVGNVASLH
                     SGFPISVIDLDKTVTLDTAGAELEIKIATAGDQYVFNASGQSIDLEGLLCAFDGQGPC
                     ANGVKDSQRTKTSAMTTRLLLLVWGVRADEERLRATVKFATELFERSGARVTPAELLG
                     GANS"
     gene            complement(9511..10710)
                     /locus_tag="VFQ61_23300"
     CDS             complement(9511..10710)
                     /locus_tag="VFQ61_23300"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957453.1"
                     /translation="MRLNPEEHRPWWYGDALGVGASGLELDGHPLTAIAAEHGTPTYV
                     YGLDSVRRRIRELRAALGAVGAPGRIHYAVKANRCRALLEVIREEGLGIDACSPREVA
                     LALEVGFTPEQISVTAGMLSNRDLEAFARAHVHLNLDTRSALRRWSEIAGPAERGVGI
                     RLDPGVRVGWGEHFAYGVSKFGFPIEQALEIVELARSLGLEVDTLHVHAGWGLKAEDE
                     ARLAHVFEQIAELSAHISSLHTINVGGGLGGRLRGEEQPLSLERWAALLRGSLGATGL
                     GIACEPGTYIAASAGVLIAEVNTVELRRSVKWYGLDVGSAVNVYAAYYGLPLEILSLR
                     DPLCTAGEPAHVAGNINEPNDVFARARPLPRFREGELVGIYPAGAYGASMASDHCMRG
                     APAEVAL"
     gene            complement(10819..11721)
                     /locus_tag="VFQ61_23305"
     CDS             complement(10819..11721)
                     /locus_tag="VFQ61_23305"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET9957454.1"
                     /translation="MTHTQNLSLDVPELRLSVFGSTDIGLVRKKNEDAFAICPFDGRA
                     TLHSLSEPLQVEVQERGVLLAVSDGLGGEKAGEVASALALHCLRSSLSHAHSASVEQA
                     LTRSVEAANREILAAGQAEERRGMGATLTAALIHGAHAYVAEVGDSRAYLLRGGRFLQ
                     LTRDQSYAELMVEAGMLRRDQINNFVYRNLVLQALGTRPEVQVGLTRVRLCEGDRLLL
                     CSDGLSGQLSDEEMAHIIQRSGDVRWVCQALIDAAKSTGGPDNITVVVAIVESGAGAP
                     CTTDRISLETIHSPSLDSLSRRMG"
     gene            complement(11777..15949)
                     /locus_tag="VFQ61_23310"
     CDS             complement(11777..15949)
                     /locus_tag="VFQ61_23310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9957455.1"
                     /translation="MRLPFLRAVLGVGPRSALVTAGLLVGSAWGCGSVPDVTNPENGG
                     QLSYGGVGNGGAGGSNPGSGGAVIAQGGRTMGQGGATDPGQNGGETGSDGPICGDSVL
                     DSTEGCDDGNSRAGDGCDGNCKVENGYACDEVGEPCTSTLSCGDGQPGPDEACDDGNV
                     RAGDGCAEDCSVESGYSCDIYGEPCTPSTNEETCGNGVTEFGETCDDGNSVSEDGCSE
                     LCQTEDGYTCSGATCVLNAACGDGTLNSGEQCDDGNRVPGDCCNGNCQLEPNCKCSTP
                     PSGGAQSCSSTIVCGDGAVNGGEACDDGNRSANDGCAADCQTVEPGFTCPPEGGACVP
                     AVVTCPNARIEAGEECDDGNAQSADGCSPNCKIEAGYVCPTVGQACRPKEYCGNGLVS
                     YTSGETCDDGNTKAGDGCSAACTLEAGYTCDTSSTPTACTKEICGNKKIAAGETCDDG
                     NQLSGDGCSSACKLELGYTCPELGAACRPICGDKRKLGIEQCDDGNRANGDGCNSRCQ
                     LEPGYVCDANGSCRKTVCGDKLKEGTEQCDDTALGAKDLPFDGCYQCLLEPSCAAGPC
                     ESTCGDGQRFSNEACDDGNTFDGDGCSSTCQIESGFACSDSVAAALPATRSLPIIVRD
                     FIGLGRETAPGASNTNYHPDFNRHLGTGIFKMVKTALSGSGKPEWRWLPYATSDVNNV
                     TQNTVYSPLSSAICSCNESAPVSSWVTTNETWLGGGEGAAKTITLLRPPCSCSNGTAC
                     TCDNSGHMFKDGGVSNSNRRNLSTPANFNQWYTDVAGVNLTTPYTLNLSLTDAATAQY
                     SNLGAPNATAFDPIGGTGGWIARAAETVSGCGTDKTVNVSFSTETHFWFEYQGGERFD
                     FSGDDDTWVFVNKKLVVDLGGLHGLQTGYFVLDSDTDGNGPDTADGSAVANGNGSFYD
                     GTNYTTSQGAKLGLGLVVGQVYELVMFQAERNQCGSNFGITLKNFGKPKSICASVCGD
                     GVVASDEACDLGKSGNTGTYGGCKTDCSLAPYCGDKAVNGAEQCDDGVNSNVYGSLSS
                     GCAPGCKVAPYCGDGHVDAVYGEACDQGAQNSSTAYGPGQCTKACQPAAYCGDGLVNG
                     VETCDEGQSNGGPASQCDTSCKIKCGNGKLDPGEQCDLGTQKNDGAYGGCKVNCVLAP
                     YCGDGTKQAPEACDDGKNDGSYGTCTATCALGSYCGDGTLDAGAGEQCDAGPQNQSNP
                     YGRELCASTCKQAPYCGDHAVDTAFGEKCDDGANNSDTQAGACKRDCSGYNSPPKTCG
                     NAKLDSGEECDNGSQNGSLGSDCDARCHYKCGNGIKDSGEACDNGVNDGSYGTCKHDC
                     TSAEYCGDGAKNGPEQCDLGDANEASPYGAGKCTTACKVAPYCGDRRLNGNEVCDGQA
                     GCNSSCTYDIVR"
     gene            16486..17682
                     /locus_tag="VFQ61_23315"
     CDS             16486..17682
                     /locus_tag="VFQ61_23315"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HET9957456.1"
                     /translation="MLGLIGTTTVAGAAFLLVRGGVDPDESFVLRADVPASSEGFALA
                     LRQSLGVELRPGHRLELLDNGRVFDALVKELNGAQSSMHLLLYIWERGAASDRVVSAL
                     IARARAGVACRILVDSFGSPKFGEEVEKPLVAAGCEVRIFRPSPSDKLARNHRKIVVV
                     DGRVGFTGGFGIRDDWLGDGVHDERWRDTNVLFAGPAVQSAQQAFAENWQEAGGQLLP
                     IEAFPEPLPVSESGTRGASDGTALAAFVSSSASPSVTRAERLTQLLISSARKRLWIAN
                     AYFVPSRAILDLLARKASEGVDVRILVPGKKSDSKTSFGVQQFEYGSLLDRGVRVWEY
                     QPSMMHSKSMVIDQNRGVIGSVNLEPLSLRKLEEGALVFESPALARALTLAFEQDCSR
                     AQELKR"
     gene            complement(18525..19142)
                     /locus_tag="VFQ61_23320"
     CDS             complement(18525..19142)
                     /locus_tag="VFQ61_23320"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HET9957457.1"
                     /translation="MATLGSFKPYSTPQIASLEAQLADTRSSDGKLSTPTKRGIRGIG
                     GLLLFGGVSLLAAALGARINAKRSNRLWYRLLRKSELTPPDRTFSIVWPILYSMSAFS
                     AWRVARAPSSPARTRALGWWGVQIACNAVWTPLFFGAHRPRLALVDLSANAASLGAYA
                     RAARDVDRTAALLVTPYLGWLSFAALLNTKVITKNTGPTAWLLRG"
     gene            19368..19988
                     /locus_tag="VFQ61_23325"
     CDS             19368..19988
                     /locus_tag="VFQ61_23325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957458.1"
                     /translation="MSKVRCKIMAICALFAAAGLSTSQEAKAEPHLHDGFYLQLATGV
                     GYYNSSAEAGANKVGFSGATLPFSLMLGGSPIPGLAIGGSLMIDYAPSPRYKINGQTF
                     DSGLDISQYVIGLGPSVDFYPDPSGGLHFQGFVGWGGLETSADGNAGGSDPTGLLLAV
                     GAGYDFWVGNEWSIGGLGRLTYAPLSMNDVTYSTIEPALLATFTYH"
     gene            20329..21384
                     /locus_tag="VFQ61_23330"
     CDS             20329..21384
                     /locus_tag="VFQ61_23330"
                     /inference="COORDINATES: protein motif:HMM:NF045039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957459.1"
                     /translation="MRVRLSFAVSSMLVAACSATDPGAPLGGLAGAAPAGGGNTALST
                     GGSSSGSGASSGGNASAGAANGGSAGTSTVVSGGKTQGEGGAPSRGGTSSVGGNSGGI
                     GSAQGGAWTGGASQGGSSQAGSSPGGASGSCPSNAAFCSSFEDSAMPAGAVFKLNGDP
                     ASPWTVNFEVDTTMAKSGKSSLRVKSANEGGGGAYKMLAVPTGGAAFWVRMYMRSDKD
                     IGEIDHNVFAQAATKTDPNDDPHMEFAEDVGLSLHYNDAEIKWPMGYGRTSSGGTTPY
                     KLVKDTWHCVELFFDGPGKTYRFYANGMEVISANGFPSASPAYSTFKFGYNSLHGTQR
                     KTWYDDVAVGPSRIGCP"
BASE COUNT         4133 a         6856 c         6577 g         4024 t
ORIGIN      
        1 ccccaagccg gcggcgtaac tcaagctcgc tctttgtacc tacaggcctc acgatgcgaa
       61 aagatcatac ctcctcgtgc ttgtcgatcc ccgtccgatc tttttctcgc ttggaaggtc
      121 agtaacttcg ctcacgtcga ctgccctcga gcccttcatg ctacacgagc gcaatcggac
      181 atacacgcct gctcgcgtgg cttccagaat gattgccaat cagcggccga agcggtgcct
      241 gcgccgcctc aatcacgccg cgcgaccgag ggcgcggcac gaggccagaa acgcattgga
      301 acgggccctc gcggacgcag ggtcacggaa ggcaccaact cgagtgcggg ttcgggtaac
      361 aactcgagtt ctacgtgctg caggatcgtg gcgaggacga gcaccgcctc ggccaacgcg
      421 aagtggctgc cgatgcacac tcgcggcccc gcgccgaacg gcaaatacgc aaatcgcggc
      481 ggcgagggtt gatcgatcca gcgcgaaggt tgaaaacgct ccggcgcatt gaagaaccgc
      541 gcgtcgtgat gcatcaccca agggcacacc acgacatgca ctcccttggg gaccagaaca
      601 ccggagatga ccacgtcctc cttgggctct cgcgccatgc cccacacggg cgggtagagg
      661 cgcaacgact cgtccagcgc cgcgcggatc cgcacgaggc ggggtacgtc ctgcatcgta
      721 ggcaggcgct cgccgagtac ggacgcaatc tcggctcgtg caacgcgcgc ctcgctcggg
      781 tggagagcca gcaagcgcaa ggcatacgtc aaggccaacg ccgtcgtctc gtgccctgcc
      841 aaaaacagag tcatcgcctc atcacgcaac gccacctcac tcaggctacc cgccacgtct
      901 cgggcgagca tcagcctgcc gagcaggtcc gcgctctgcg aggcctgttc accagagcgt
      961 tgctgttcag cggagctctg ctgctgtcgg cgttcggcga tcaacgagtc gagaatgacg
     1021 tcgagctctc gccgggttcg agccaggcgt cgtcggctcg gcagcgggac ccattcgggt
     1081 accagccgcc acgccaaaga acccggacga taatcatcga cgaagggcgc caacaggcct
     1141 tccacttctt tcgcctgctt cacttccatt ccaaatagcg cccgcaccaa tatatcgaga
     1201 gtcaggtgca tcatgctcga gtgcacgtcg aagacctcgc ccgcgcgtcg ggtgaccacg
     1261 aactctcgcg ctcgctcggc catgattccg ccgtatgcgg ctatctcatt cggctgaaat
     1321 gaagaagcca tcagcttcct acttcgtcgc cattcctcgc cttcactcgt caacaggccg
     1381 gtccccagaa tgcgacgcaa ctccttggtg aaccgatcct tctgaaaggc cgcgtgttgc
     1441 gtgacgagca cttgttccac cgctttgggg tcgaacagaa ccagaaagcg gtcccccagc
     1501 acctcgaatg cggtcgcatg ctccgaagcg gcgcgagcgt gctccaagcg tagcagtggc
     1561 ctgccccgat acctgaggat cgatcgcacc ccggagagtg cgggcagcgg cgcgaagggc
     1621 tcaccagtga ccggtggtgc aggctctgcc acgacgctcg ccgccgccgc gcccgttcct
     1681 cgcgagcctg actttcccaa gcctgagtct ccggtacccg cgttcacgcg cgctgcatcg
     1741 atgtgcgctt cgttcatgcg cgcagtctac tcggttcaag ctgcacttga gcaggcagaa
     1801 agctcgatcg ttactgcgtt ttgttggcgg ccacgcagaa acgggcgcgc cgcgcggtga
     1861 agcccgccca cggcgcagcc tctttccgac agtcctcccc agatcaccgc gcaggccccc
     1921 ccgacaacgc cagccacttt tcccgttgac ccttcagatc gcggccccca cactgcgagg
     1981 acttcgcgag ctcgcccacc gcaaacgcag gaccacatgg ccaagactcg acacgcgacg
     2041 gtcgtcactc cgctccccca ggagctcctc cttctttcgt tcgaggcaag ggagcagctc
     2101 gcccagccct tcgaatatca actggagctg ctcgcggaga gcgatgacgt cgacttgtcc
     2161 aagttgctcg gcgagtcagt gcgggtggag ctcgagctgg cggatggcaa gacacgcgag
     2221 ttcaccggct tcgtgaccca cgcgagcgcc ctcggccaag agggtcgcta cttcaagtat
     2281 cgcgccgtca tgcggccgtg gctctggttc ttgacccaat cctgggattg ccgcattttt
     2341 cagaaggaga ctatcccgga ggtggtcaaa tccgtgtttc ggcagcacgg attcacggat
     2401 ttcgagaccg agctcacagg gcagtatcga agtctcgaat atatcgtcca gtatcgtgaa
     2461 tccgatttca acttcgtcca gcggctactc gagcaggagg gcgtctatta ctacttccgg
     2521 cacgagaagg gccgtcacaa gctcgtgctc tgcgactcca tcagcgcaca cgcgccgaag
     2581 ccaggttatg agtccatccc ctactatccc ccccagcccg gtgagcaccg cgagcgggat
     2641 tcactcgatg cctggctggt ctctcgtcag ctacagtcgg gatcgctcga gcttcgtgat
     2701 ttcgacttcg agaagccacg ggcagagttg ggctaccgct cggagacgcc gctcggccac
     2761 gcccacgact actacccccg ctatgactac cccggggtat tctcgaatcg agccgaagcc
     2821 gagacccaga cgcgggttcg gctgcaggca atccaagctg ggttcgagtt ggtgcgcgca
     2881 accgggaatg cacgagcgat gtgcaccggc tatctcttct cgctcgagaa gtacccccgc
     2941 gaagatcaga acaagcagta tctgatcctc gagacgaatt acgaaattca cgtcaacgac
     3001 tacgaaacga cggggaacaa ctccgaccgc ccggagtgcc gggtggagct cgtcgccctc
     3061 gacgccaaac gcgtatttcg ccccgaacgg cgcgcgcgta agcccatcgt cgaggggccg
     3121 cagaccgcaa tcgtcgtggg ttatagcgga caagagatct ggaccgacaa gtacgggcgg
     3181 gtcaaagttc aatttcattg ggatcgcgcc ggaaaatcca acgagacgag ctcgtgttgg
     3241 gtgcgcgtat cccagctgtg ggcggggagc gggttcggtg gcatccacat ccctcgcatc
     3301 gggcaggaag tgatcgtgga cttcctagaa ggcgacccag accgccccat catcacgggc
     3361 cgagtctaca atgcggacaa tatgccgccc tatgacttgc cgaggaatca gacccaaagc
     3421 gggatcaaga gtcgaagcag taagggcggc ggcgcggaga actgcaacga gattcgcttc
     3481 gaggatctaa agggttccga agagctcttc attcaagccg agaaggacca gaataccaac
     3541 gtcaagaaca atcaaagcat cacgatcggg gtgaaccaaa ccgaaaccgt gggtgccaat
     3601 cagacgctga cggtgaaggc caatcaaacc attacgacgc tggccaatcg cacggtcacc
     3661 gtgctcgcca acgaggccat tcaagtgagc ggcacgcgca gcaccaacgt gaccttggcg
     3721 gatacccaga cctacgccgc cactcgcacg gtgcaagtgg cgcaggccga cacgctcacc
     3781 gtcaatggtg gacggaacgt ggccgtcacc ggcgcggata cgacgacagt gtcacagggc
     3841 ggcaagacaa ccagcgttcc ggatggagcc tatgggattg acgccaagtc ttacggtgta
     3901 gactcccaaa gtagcctctc actcacctgt ccgagcgcta gcatccaagc gggcccgccc
     3961 gcgccaggcg cgccgagcgg ggggcccccg accgcactca ccttgatcgc acaagatgcc
     4021 ctgatcctgc agtgcggcga aaacgtcatt tatatcggga aagacgggat cagcatctcg
     4081 agcccgagta gcgtgacggc gctggtgggt gacaacgcgc tcgacctcgc gaaggattgc
     4141 gcgacgctca ctggtaagaa cgccaccgtc acgtccagcg gcagcaccga tgtgatgggc
     4201 ggccaagtaa atctacgcaa ggctccgccc aagaaggtga tcaagaaggt ttagccgaaa
     4261 gcacctcaac aactcagggg aggataccga tggatcgaga tgtcgtcacg attgggctga
     4321 gagcgcgctc gctcgccgct gcctatacgt tgagtgcatt ggcgggcctg tggggctgcg
     4381 ccgctgatac gaaccgccac gccttggggc tggcttcacc ctcggccgcc aacgaccggc
     4441 ccgcggacag cggcgagagc gccgccgcca gcgctccgcc ggtacgccat gactcggcga
     4501 cggaacgcgc tgcgctcgac gcctgttacg ccgcgtccag cgcgcagcat cctgggttgt
     4561 ccgtgaacag taccgctttg tacttggtac gtggtggcaa gtacatctat gtggatttcg
     4621 agttggccga ggcaccagaa ctcgcgcgct gcatccgcga aagcatgctg cagtggcaag
     4681 cattccagcc tcctggatcc gatccaggcg ccgttggcgt tggcgggcgc ccgatccggc
     4741 ttggacccgc cccgacggaa aggcccgccg cggaaacatt cgccgcacac cgcgagcgat
     4801 ggcgccgcgt cgtcaactca gccctggaaa agggcatact ggaccgcaac gaccccatcg
     4861 tgcaggagac tctccaccca aagccggagt ggcccagtca ggcgcagcgc gcgcagctcg
     4921 agagttgcta cgcgaaccac gtttcaacga agccaactcc tgagctgcat ctagaagcca
     4981 tatacctcgc gcggggggca cgggtactac tggcggatgt actgcttccc gatccgaagg
     5041 ccccacgcgc acccgagttt gccgcgtgcc tagcggggcg tatacagagc tggccggccc
     5101 ccttcgaagg cacgcgggag actgtattca gctcgttctt cattcacttg ggcacgaaca
     5161 aagaagaatt tcccaacctg cctgcttcac cggagctact cctggcgcgg cgtagaggat
     5221 tgattctgag ggctgctgac ttgaggctcg tcagccaaga cgatcccgta tgggcgctcc
     5281 tgaatcagca gcccggccta cgcaaatagg caagcaagcg agcgaaagcc gaggacccgc
     5341 cgatcctcac tttctgtcgt ccgttgcgcg gaacgagatc gaaacgcggt ctgctaacga
     5401 gcgaaacaaa taagagacag gcgttactcg cgatcagcgc gccgcgtacc tcgctcagca
     5461 cacgacccat ccagtgttgc ggagtcgggc gttgatggac acatcggcaa tggaagtagt
     5521 atcgtgcctc acgacagcga ctcagcgctg aaccacgaaa ccaaacacaa tgaaactctc
     5581 cgcacgtaac gtcatatccg gcaaggttat cgctatcacc aagggagtta cgaccgccca
     5641 tatcaagctg gagattgcgc caggttccgt gattaccgcg tcgattacga atgaagcggt
     5701 cgatgacttg gggctcgcgg tgggccgtga agcgacggca atcatcaagt cttccgacgt
     5761 tatcatcggt gtgacttgaa tcagagcttt gtcgggattc cgacaaagct ctgtcgagga
     5821 cttggcatgg gctagcgcgc ttatcccgtc gtgaacgact ggtggcggga ggtgtccccc
     5881 cgcgtgcgtt cggcgcggct ctgaggctcc gcatcacccc aagcgaggtt cgcatgccaa
     5941 caccggcttg atgtcgacga ccggggttcc gtcgatcgct tcgatagggc caatccgaag
     6001 ccgtccatgt tcgatgctgc gaacgtggac gggatgcagg cccaggggat tgggccgatc
     6061 gggagacctg gtcgcgaaga cgcctcggat cgcgttctgt ggatccgagc gcggatgtac
     6121 tttgaggatg tcgcgctgag ccagatgaaa ccaggtgatc aataggatct cgtcgcccac
     6181 tgcgagtccg tcgacggcgt cgattgccca cggctcgatt tccaaccaag cgtcgggcgc
     6241 accctccgaa ccctgcttgg gggcgtcgcg acgcgcagtg agctgggatc gaatagtacc
     6301 gatcgggcgt aacaggaaca gcccgctaac tgaagggtcg aatgactcag ccatgatgtc
     6361 taggtacgat tgacacatct cgggcccgcg tgacacgtcg cctcttgcgc ggagcgcccc
     6421 ggcagggcaa gcaagtgccc tgccatggac atcaagaaga ccatcgccgc tttgaccttg
     6481 gaagagaagg tggctctgtg ctccggtcag gacttctggc gcaccaaggc aatcgaacgc
     6541 ctgggcatcg tatcctacat gctgaccgac ggcccccatg gtcttcgcaa gcaaaccagc
     6601 gaggcagaag ttgctgccct gcacgactcg gtgccggcta cctgttttcc ctccggcgcc
     6661 ggactggccg caacctggaa taggtcgctc ctggaagaag tgggcgaagc cttgggtcgc
     6721 gaggcgcgcg ccgaagacgt cggagtgctg ctcgggcctg ccatcaatat caagcgcagc
     6781 ccgttgtgcg gacgcaattt cgagtacttc tcggaggacc cgtacttggc tggcgagctc
     6841 gcgaagtacc acgtccgagg cgttcaagcg caaggagtcg cagcctgccc gaagcacttc
     6901 gccgccaata accaagaaaa ataccgcatg ttggtcgacg ctcgggtgga cgcgcgcagc
     6961 ttgcgcgaaa tctacctgcc cgccttcgaa agcacggtaa aagacgctcg gccttggacg
     7021 ttgatgtgcg cctacaaccg gctcaacggc gtctactgcg cgcaaagcgc ttggttgttg
     7081 aatcaagtgc tcagggatga atggggctac gatggagtcg tggtttcgga ctggggggcg
     7141 gtcgatgatc gggtagagtc cctgcgtgca gggttggagt tggagatgcc gggaaatggc
     7201 gggattacgg atcgtgagct cgcggatgcc gtgcgcgccg ggaaactcga tgaacgggtg
     7261 ctcgatacgg cgctcgaacg tttgctcaca ctctattccc gcgttcaaga cgggcgccaa
     7321 ccgggcaccc cgttcgaccg cgaggggcac cacgcgttgg cccgccgcgt ggcacaggag
     7381 agcttggtgt tgctcaagaa ctcgaatgaa ctgctgccgt tgcctcaagc gggtcgcatt
     7441 gcgttcatcg gaaccttcgc cgcagagccc cgctaccagg gcggtgggag ctcgcatatc
     7501 cagccgaccc gtgtagagaa cgccttggac gcggcccgcg agctgttggc aggttccgct
     7561 gacgtagtct ttgcgcgcgg atacgacgtc cgatcatccg cgcccgatcc gacgttgatc
     7621 ggcgaagcgg tcgagcttgc gcgtggagcg gacgcagcag tcttattcgt cgggctaacg
     7681 gataccttcg agtccgaagg tttcgatcgc gctcacctgc gcatccccga aagccatgtc
     7741 gcgctgatcg atgcggtcgc cgcggtgcag ccccgaactg tcgtcgtatt gtccaacggc
     7801 tcgccgatcg aaatgccctg gctcccgcgc gtgggcgcgg tcctggaggg gtacttggga
     7861 gggcaggccg gcgggggagc cgcggccgac atactgttcg gtgaagccaa tccatcgggc
     7921 aagctgcccg agacctttcc ggttcgcatt caggacacgc cctcttacct caactttccc
     7981 ggcgagaagg accgcgtaga gtatcgcgaa ggtctgttcg taggataccg tcacgctgac
     8041 tccgccgaag tggagcccct gtttccgttc gggttcggcc tctcttacac gaccttcgct
     8101 tactccgaac cccacgtgga tcgcgaggag ttgcaggaac acgaggaact gacggtaagc
     8161 gtgcgcgtaa agaataccgg caaacgagcg ggtcaagagg tcgttcagtt atacgttcgg
     8221 gacgaagtca gctccgtagt gcgcccctac aaagaattgc gcgcgttcga aaaagtggcg
     8281 ctcgagccag gagaggagaa agaagtccga ttcaacttgg gcaaacgcgc ctttgcgttc
     8341 tgggacgacc gacagcacga ctggcgcgtg gaaccaggtg aattcacgat tttcgtaggt
     8401 gcgtcgagtc gtgatctgcg ggggcgagtc aaagtcacga ttcgaaatgc cactcagccc
     8461 gttccgcact gggaccagaa ttcattgctg ggtgacgtga tggcgcaccc ggtggtcggc
     8521 ccggccatca ccgcgctgaa gaatgagttc tccaaagcgc tgggccaata ccctcccgat
     8581 tcagcagaag ccttgatggc ggaggccgtt gcacgtgaga tgccgctgcg gaacgtggtc
     8641 cgcatgctgc gcgggctgaa tcaggaacaa ctcagcttgc tgctcgacgt actaaacggt
     8701 gcaacaccgc cagagcgcct gctgtccgag ctgcccacgc accggtagca ttcccaaaga
     8761 cctcgtgagt cgtcgctggt ctgcctcgcg aggaccggcc ctgcgagttg atccaagtac
     8821 tgccgccgcg gtattctccc gaacgagatg ttgctcgtgc acccgcatcc cctgctcgct
     8881 ctaggagcaa ttcaggttga atactcgagc ccgctgagcg aacagccaga cctcgcttgg
     8941 ctttcggagc tcctgaagcc aggagcggca accccgctcg cgcccgtcac ggaccgcctg
     9001 cgttcgagcg ttcgcgatct gctccggcat ggtggataca agccgaccgg gcgcggcaaa
     9061 cccgcatccg aatatctagt ccgcgccgcc gacgaaggga gtttgaagcc catcaacccc
     9121 gccgtggatg tcggcaacgt cgcgtctctg cactcgggct ttccaattag tgtgatcgac
     9181 ttggacaaga ccgttacctt ggacactgcc ggcgccgagc tcgagatcaa gattgcgacc
     9241 gccggcgatc agtacgtatt caacgcttcc ggtcaaagca tcgatctaga gggtttgctc
     9301 tgcgcattcg atgggcaagg gccctgcgcc aacggagtca aggattcgca gcgcaccaaa
     9361 accagcgcaa tgacgactcg cttgctgctg ttagtctggg gtgttcgagc ggatgaggag
     9421 cgacttcggg ccaccgtgaa gttcgcgact gaactcttcg agcgttccgg agcccgagtg
     9481 acgccggccg agctgctcgg aggggccaac tcatagcgcg acctccgcgg gcgctccccg
     9541 catgcagtgg tcactggcca tgcttgcgcc gtaggcgcca gcgggataga tgccgaccaa
     9601 ctccccctct cgaaagcgcg gtagtgggcg cgccctcgcg aatacgtcat tcggctcgtt
     9661 gatgttgccg gccacgtgcg cgggctcgcc cgccgtgcaa agcggatcgc gcagcgacaa
     9721 gatctccaag ggcagcccat agtaggcggc atagacattc acggcgcttc ccacatcgag
     9781 gccgtaccac ttgacgcttc gacggagttc caccgtgttc acctcggcga ttagcacgcc
     9841 cgccgacgct gcgatatagg tcccgggctc acaagcaatg ccgagaccag tggcacctag
     9901 agagcctcgc agcagcgccg cccagcgctc caagctcaac ggctgttctt cgccacgcaa
     9961 acgcccaccc agcccgcctc cgacattgat ggtgtgcagc gaagaaatgt gagcggagag
    10021 ctccgcaatt tgctcgaaca cgtgggcgag ccgcgcctcg tcttccgcct tgaggcccca
    10081 tcccgcgtgg acgtgcagag tgtcgacttc gagccccaag ctccgcgcga gctccacgat
    10141 ctcgagcgcc tgttcgatgg ggaatccgaa cttgctgacg ccgtatgcga agtgctcgcc
    10201 ccagccgaca cgcacgcccg ggtccaatcg aattcccacg ccgcgctcgg cggggcccgc
    10261 gatctcggac cagcgcctca gcgccgaacg agtgtccagg ttcaaatgca cgtgagctcg
    10321 ggcaaacgct tcgagatctc gattggagag catcccggcc gtcaccgaga tctgctccgg
    10381 ggtgaagcct acctcgaggg ccagcgcgac ctcgcgcgga gaacaggcgt cgataccgag
    10441 cccttcctcg cgaatcactt cgagcaacgc gcgacaccga ttggccttca ccgcgtaatg
    10501 gatgcgcccg ggcgcgccca cggcaccgag cgcagcccgc agctcgcgga tccgacggcg
    10561 cacgctgtcg agcccataaa cgtaagtcgg ggtgccatgc tcggccgcga ttgctgtcag
    10621 cgggtgacca tcgagctcca acccactcgc accgactccg agggcgtccc cataccacca
    10681 aggcctgtgc tcttcgggat tcagacgcat accgggcaga caggatagtc cactgcgcgc
    10741 gcacttggaa cgctccggtg cctctgcctg cccgggcctg acgccggccg gtacgggcgg
    10801 ggtggactca aaaattcttc aacccatccg gcggctcagc gagtcgagcg acggtgaatg
    10861 tatggtttct agcgaaatgc gatccgtggt gcacggcgcg ccggcgccgc tctccacgat
    10921 cgccaccacg acagtgatgt tgtccggacc gcccgtggat tttgctgcgt caatcaacgc
    10981 ctgacagacc cagcgcacgt ctccggatcg ttgaatgatg tgcgccatct cctcgtcgct
    11041 gagttgccca gacagtccgt cggaacagag caagagccgg tcgccttcgc acagccgcac
    11101 cctcgtgagc ccaacctgca cttcgggccg agtgccgagg gcttgtaaca cgagattgcg
    11161 ataaacgaag ttgttgattt ggtcgcgccg cagcatgccc gcttcgacca tcagctccgc
    11221 atagctctga tcgcgcgtga gctgcaagaa cctgccccca cgcagcagat acgctctcga
    11281 gtcgcccacc tcggcgacat aggcgtgagc gccgtgaatg agtgcagccg tgagggtagc
    11341 gcccatacca cgccgctctt ccgcctgacc ggcagcaaga atctcacggt tggccgcctc
    11401 cacgctgcgg gtcagcgctt gctcgaccga tgcgctatga gcgtgagaca agctcgagcg
    11461 taggcaatgc agagcgagcg cgctcgctac ctcgcctgcc ttttcgccac ccaacccgtc
    11521 ggagacggcc agtagcactc cccgctcctg cacctccact tggagcggtt cagacaacga
    11581 gtgaagggtg gctcgtccgt cgaacgggca aatggcgaac gcgtcttcat tctttttccg
    11641 caccaaaccg atatcggtac tcccaaacac cgagaggcgc agttcgggaa catccaacga
    11701 gaggttttgg gtgtgagtca tcaccggacc tcgaagccag atccgtgcca aagtgtgcaa
    11761 aactgagggc tcgccttcaa cgcacgatat cgtacgtaca ggacgagtta catccggctt
    11821 gcccatcgca aacctcgttc ccattgagcc gacggtctcc gcaatatggg gcaaccttgc
    11881 aagctgtcgt acatttgcca gcgccgtagg gtgaggcctc gttcgcatct ccgaggtcgc
    11941 actgctctgg tccgttcttc gcgccgtcac cgcagtactc ggccgaagtg cagtcgtgct
    12001 tgcaggtccc gtaagagcca tcgttcacac cgttgtcgca cgcctcgccc gaatccttga
    12061 tgccattgcc gcacttatag tggcagcgcg cgtcacagtc gcttcccagg ctgccgtttt
    12121 gggacccgtt gtcgcattcc tcacccgagt cgagcttcgc gtttccgcag gtcttgggcg
    12181 gtgagttata acccgaacaa tcccttttgc aggcgccggc ctgagtgtcg ctattgttcg
    12241 cgccgtcgtc gcacttctct ccgaacgcgg tgtcgacggc gtggtcgcca caatagggtg
    12301 cctgcttgca ggtgcttgcg cacaactcgc gaccgtaggg attggactga ttctgtgggc
    12361 cagcgtcaca ttgctcgccc gcgcccgcgt cgagcgtgcc gtcaccgcag tacgagccga
    12421 gcgcgcaggt cgcggtacag gtgccatagc tgccgtcgtt cttgccgtcg tcgcaggcct
    12481 ctggcgcctg cttggtcccg tccccgcaat agggagcgag cacacagttg actttgcagc
    12541 cgccatacgc gccatcgttc ttctgggttc ctaggtcgca ctgctcccct ggatcgagct
    12601 tgccgttgcc gcacttgatc ttgcaggacg tgtcgcattg gctggcgggt ccaccattgc
    12661 tctggccttc atcgcaagtc tccacgccgt tgaccaaacc atccccgcag taagccgctg
    12721 gttgacacgc tttggtgcat tgcccggggc cgtatgcggt gctcgagttc tgagcgcctt
    12781 ggtcgcaggc ctctccgtat acggcgtcga cgtggccatc cccgcagtac ggagctacct
    12841 tgcagcccgg tgcgcaaccg ctgctgagcg aaccatagac attcgaattc acgccgtcat
    12901 cacattgctc tgcgccgttg acggccttgt ctccgcaata gggcgcgagc gagcagtccg
    12961 tcttgcagcc gccataggtt ccggtattcc ccgacttgcc gagatcacac gcctcgtccg
    13021 aagccacaac gccatcgccg cacactgaag cgcagatcga cttgggctta ccgaagttct
    13081 tcagtgtaat gccgaagttc gagccgcact ggttgcgctc ggcttggaac atcacgagct
    13141 cgtagacctg acccacgacg agccctaggc cgagtttggc gccttgggat gtggtgtagt
    13201 tggtgccatc gtaaaatgag ccattgccgt tcgcgacggc gctgccgtcc gcggtatccg
    13261 ggccgtttcc gtcggtgtcc gagtcgagga cgaagtagcc ggtttgcaga ccatgcaggc
    13321 cacccaaatc cacgacgagc ttcttgttca cgaacaccca ggtgtcgtca tcgcccgaga
    13381 agtcgaagcg ctcgccacct tggtattcga accagaagtg cgtctccgtc gagaaggaca
    13441 cgttgacggt cttgtccgtc ccgcacccgg agaccgtctc cgccgctcgc gcaatccaac
    13501 cgccggtgcc gccgatcggg tcgaatgcgg tagcgttggg tgcccccaag ttcgagtact
    13561 gtgccgtcgc cgcgtcagtc aagctcaggt tcagagtgta tggcgtggtg aggttgaccc
    13621 ccgcgacgtc cgtgtaccac tgattgaagt tggccggcgt cgacaaattc cttcgattgg
    13681 agttactgac accgccgtct ttgaacatgt ggccgctgtt gtcacacgtg catgcagttc
    13741 cgttcgagca cgagcatggt gggcgcagca gggtgatggt cttcgcggcg ccctctccgc
    13801 cgcccaacca ggtctcgttc gtcgtcaccc aagatgaaac gggcgcactc tcgttgcagg
    13861 agcagattgc gctactcagg ggcgagtaca cggtattttg agtgacgttg ttgacgtcgg
    13921 aagtggcgta aggcagccaa cgccactcgg gtttaccgct gcccgagagc gcggtcttca
    13981 ccatcttgaa gatgccggtg cccagatggc gattgaagtc gggatggtag ttggtgttcg
    14041 aagctccagg tgccgtctcg cgcccgagcc cgatgaagtc tctcacgatg atcggaaggc
    14101 tcctggtcgc tggcagagct gctgcgaccg agtcgctaca agcgaagccc gactcgattt
    14161 ggcacgtgga cgagcaaccg tcgccatcga acgtgttccc gtcgtcgcag gcctcgttcg
    14221 agaacctctg gccgtcacca cacgtggact cacagggccc ggccgcgcaa ctcggctcga
    14281 gtagacattg gtaacagccg tcgaagggca agtccttggc gcccagcgcg gtatcgtcgc
    14341 actgctcggt gccctctttc agcttgtcac cgcacaccgt cttccgacag ctgccgttcg
    14401 cgtcgcagac atagccgggc tcgagctggc agcgagaatt gcaaccgtcg ccgttggcgc
    14461 gattaccatc gtcgcactgc tcgatgccta gtttgcgctt gtcaccacag ataggtcgac
    14521 acgcggcgcc cagctccggg caggtgtaac cgagttccaa cttgcatgca gagctgcagc
    14581 cgtccccgct gagttggtta ccatcgtcgc aggtctctcc ggccgcgatc ttcttgtttc
    14641 cgcagatctc cttggtgcaa gccgtggggg tggagctcgt gtcgcacgtg tagccggcct
    14701 ccagcgtgca cgccgcggaa cagccgtcgc ccgccttggt gttcccgtcg tcacaggtct
    14761 cgccgctcgt gtagctgact agcccgttac cacagtattc tttcggtcgg cacgcctgcc
    14821 ccactgtcgg gcacacatac cccgcttcta tcttgcagtt cggggagcag ccatctgcgc
    14881 tctgggcgtt gccgtcatca cactcctcgc cggcctcgat gcgcgcgttc ggacaagtca
    14941 ccacggccgg gacacacgca ccaccctccg gcggacacgt aaacccgggc tccacggtct
    15001 ggcaatcggc ggcgcagcca tcgttcgcgc ttcgattgcc atcgtcacaa gcttcaccgc
    15061 cgttcacggc gccgtcgccg caaacgatgg tcgagctgca gctctgcgcg ccgccgctgg
    15121 ggggtgtact gcacttgcaa ttgggctcga gctggcagtt cccattgcag caatcacccg
    15181 gcacgcggtt gccgtcgtca cactgctcgc cgctgttgag cgtgccgtca ccacaggctg
    15241 cgttcaatac gcaggtagct ccgctgcagg tatagccgtc ctcggtttgg cagagctcgg
    15301 aacagccgtc ctccgatacc gagtttccgt cgtcgcaggt ctctccgaac tcggtgacac
    15361 cgttgccgca cgtctcctca ttggtcgagg gtgtgcatgg ttcgccgtaa atatcgcagg
    15421 aatagccgct ctcgacgctg cagtcctcgg cacaaccgtc gcccgctcgg acgttgccat
    15481 catcgcaagc ctcatcgggc cccggctggc cgtctccaca cgagagcgtg ctcgtgcagg
    15541 gctcgcccac ctcgtcgcag gcgtagccgt tttcgacttt gcagtttccg tcgcagccgt
    15601 cccctgctcg cgagttcccg tcgtcgcagc cctccgtgga gtcgagcacc gagtcgccgc
    15661 agattggccc atcactgccc gtctcgccgc cgttctgccc cggatcagtg gccccgccct
    15721 ggcccatagt gcgccctccc tgggcgatga ccgctccgcc actgcccggg ttcgagcctc
    15781 cggctccgcc gttgccgacg ccaccatagg atagctggcc accgttctcg gggttggtga
    15841 cgtccggcac agaaccgcag ccccaagctg acccgaccag tagccccgct gtaaccaaag
    15901 cggagcgcgg cccaacaccc aataccgcac gcaagaaggg aagacgcatt ggaatcaacc
    15961 cacaaggatt cacaatcact cgctcaaatc caactcgttt tcgttggctc gccacattta
    16021 gtggtcgagt cagaacgccg cgccgacatt gtgtgaagct ccgcgcacac cgtacgatcg
    16081 agcccgtgtt cgtatggtcg tgagcaatcc acgctaccct ccggagtaga tagcgaagta
    16141 tcagacattg accgagcggg ttacgacgaa gctcggggcg gtgcccctaa gactctcaga
    16201 gactggtctg cacccaggtt acgcgctcgg aatggcgtcc tcgagaatgg tcaacttctc
    16261 ctcggtctct cggacgcgct cgggaagcgc gctcaaaaat cgtccgcagc ggctggttga
    16321 aatcgcgcac cccgagattc actgaaaggc tttgcgcgag gatgtgagtg acgtacggtt
    16381 tgcgacgtgg tcgctctcgg ttctggtcgt gacggaacgg ctgaaatcgc cgaacctcga
    16441 gttgggcgat ctgccgtctc cagcgcgctg cgccgatgga agtgggtgct cggactgata
    16501 gggaccacca ccgtcgcggg cgcggccttt ttgctggtgc gcggcggggt cgatccagac
    16561 gagtctttcg tcttgcgtgc cgatgttcct gcgtctagcg aaggcttcgc gctcgcgctc
    16621 cgtcagagcc tcggcgtgga actccggccc gggcatcgat tggagctgct cgacaacggg
    16681 cgagtgttcg acgccttggt gaaggagctc aacggggctc agtcgagcat gcatctgctg
    16741 ctctacattt gggagcgcgg cgcggcgtcc gatcgcgtcg tgtccgcgct gattgcccgg
    16801 gcgcgagctg gcgtcgcttg ccgaatattg gtggattcgt tcggtagtcc caagtttggc
    16861 gaagaggtcg agaagccgct ggtggccgcc ggctgtgaag tgcgcatctt ccgaccgtca
    16921 ccctcggaca agttggcgcg caatcaccgg aagatcgtag tagtcgacgg tagagtcgga
    16981 tttacgggcg gatttgggat tcgggacgat tggctgggcg acggggtgca cgacgaacgg
    17041 tggcgtgaca ccaacgtgct ctttgcggga ccggccgtgc aaagtgcaca gcaagccttc
    17101 gctgaaaatt ggcaagaggc tggcggacag ctgttgccta tcgaagcgtt tcccgagcca
    17161 ctcccagttt cggagtctgg gacgcgcgga gctagcgatg gaaccgcgct cgccgccttc
    17221 gtgtccagca gtgcctcccc cagcgtgacg cgcgccgagc gcctcaccca gctgctgatt
    17281 tcttcagcgc gcaagcggct ctggatcgcg aatgcctatt tcgtaccgtc gcgcgcaatc
    17341 ttggatctcc tggcccgcaa ggccagtgaa ggagtagacg ttcgcatcct ggtcccgggc
    17401 aagaagagcg attccaagac ttcgttcggc gttcagcagt tcgagtacgg gagcctcctc
    17461 gaccgtggcg tacgggtttg ggagtaccag ccgtcgatga tgcattccaa gtccatggtg
    17521 attgatcaga accgcggagt catcggttcc gtgaacctgg agcccttgtc attgcgcaaa
    17581 ctcgaagaag gagcactcgt tttcgagtcg cccgccctgg cccgtgcact cacgctcgct
    17641 ttcgagcaag attgcagccg tgcccaggag ttgaagcggt agttagcgtg cagtcgcgcc
    17701 ctattcacac tacacgactg gccgcgtact cgggcggata gagagctctc cgcaatccag
    17761 tcccaacgcg gtgccgttgg aacctcggta tgcgccggct cgggggagga agccgctcgg
    17821 atagtaatgg ttttggacgt tcgttcgggc tcttgcaaaa tgccgcgatc gacgtcagac
    17881 atcgtgggcg actaaacgag ggaagtgagc gcgagcaata gcctcgtaag ccctaaacag
    17941 ggcgacccgt aaagcccaga gagccttaac accgccgcgc aagtgactag actacgagaa
    18001 cacgccgcaa tgagcggcaa atcgatacag gaggtggatt catgaaccat tttcgtcccg
    18061 ccagttcttt gtacttgagt tttaccctag ccttggctgc atggggctgc tccgcggctt
    18121 ccgacgaggt ctcttccgag cctacggtag gagtgtccag cgccgcgcta gctcaaagct
    18181 gtagtgtgcg cggggacccc ccgctgacct ccggctgcag tgaaggtcag tattgcagac
    18241 tcgaagcgtg tacgaactca attccagctt cctgttgggg aacgtgccag accaagccga
    18301 agcgagcaat tctcccccca cccgacaaat gctcgggcag cttcgcttgt ctttgcggca
    18361 caccgaagtg cgttggaggc gaatggactt gcgaagggag ctgtgggccc ggcggcgaga
    18421 cggagtgaag gcggtcgcgc cccgagtctg gagcccaaag tagcctgttg agccaggtct
    18481 agattcgatc aaacgaggtt cgagctgcag cgcgatgaat tcgattagcc gcgcagcaac
    18541 cacgcggtgg gtccagtatt cttcgtaatg accttcgtat tgaggagcgc cgcgaacgaa
    18601 agccagccca gataaggggt aaccaggagc gccgccgttc tatctacgtc gcgagctgca
    18661 cgcgcatagg cgcctaagga ggccgcgttg gcgctcaagt ccaccagggc cagacgcgga
    18721 cggtgcgcgc caaagaagag gggcgtccag actgcgttgc atgctatttg gacgccccac
    18781 cagcccagtg ctcgagtgcg ggcgggcgag ctgggcgcgc gggctacgcg ccaagcggag
    18841 aacgcggaca tcgagtacag aatgggccac acgatcgaga aagtgcgatc aggaggtgtg
    18901 agttcagact tccggagcag ccggtaccag agtcgattgc tccgcttcgc gttgatgcga
    18961 gcgcccagcg cggccgcgag cagggagaca ccgccgaaga gcagcagtcc accgatccct
    19021 cggatccccc tcttcgtcgg ggtgcttagc tttccatcgg agctgcgggt atccgccaat
    19081 tgggcctcga gcgaggcgat ttggggagtg ctgtacggtt tgaaagagcc gagcgtcgcc
    19141 attcgccggt gtgaagcaag cggcgcgcca gcagctccct cgactcggcc cagctcgacg
    19201 tcggccggct cgacgtcggc cagctcgaca tcacgccgat gacgcgagcg ctcgattcac
    19261 tagaaccagc gttcacacaa ggtgcgcaag cgaagcaccc gtgcggacaa accgggcggc
    19321 gcgagcatgg tccttatcac gacatagccc aagtcgtttc aggttgtatg tcgaaagtcc
    19381 gatgcaaaat catggcgata tgcgcgctct tcgcggccgc ggggctgagc accagtcagg
    19441 aggcgaaagc agaaccgcat ttgcacgacg gtttctatct gcagctcgcg acaggagtag
    19501 gttattacaa ctcctcggcg gaagcgggag ccaacaaagt cggattctcc ggcgccacgt
    19561 taccgttctc cttgatgctc gggggttcgc ccatcccggg tctggcaatc gggggcagct
    19621 tgatgatcga ctacgcgccg tctccccgct acaaaatcaa cggccagacc ttcgactcag
    19681 gtctcgatat ctcgcagtac gtcattgggc tggggccctc cgtcgatttc tatccggacc
    19741 cgagtggtgg gctccacttt cagggattcg taggttgggg aggcctcgag acttccgcgg
    19801 acgggaacgc aggtggcagt gacccgaccg gtctcctgct ggcagtgggc gccggctacg
    19861 acttctgggt aggcaacgag tggagcattg gcggcctggg ccgcctcacc tacgctccgc
    19921 tgagtatgaa cgatgtgact tactcgacga tcgagcccgc gctcctcgcg acctttacct
    19981 atcattgagt ttgcagacag agtccatatg cgcccgccgg tcgcacgttg cgactggcgg
    20041 ggccgcggcg cacttttgcg gtcccaggcg ccgtcttccg atcgcgcaac gacctgagag
    20101 tgggggccac accgcaggcc gaaaacctgg aaccgggccc acagtgatgc tcgaacgagg
    20161 ttaccgcaat cgccccaacg ccgagcatgc gctgactcgg atgtttgttc gtttaacgcg
    20221 cagcgatgaa cggcacgatc ctcagagtgg gttatacctg ccccacgcgt cccaatgcgc
    20281 tgagcagtgt cgattatgaa attacacgaa tcttctttcc gccattcaat gcgggttcgg
    20341 ttgtctttcg cggtttcttc gatgctcgtt gcggcgtgct ccgcgactga tccgggagcg
    20401 ccactcgggg gacttgccgg agcggcgccg gccggaggag gaaacacggc gctgtccacc
    20461 ggcggaagct cgagcggcag tggtgcctcg agtggcggga acgcgagcgc aggagccgca
    20521 aacggcggca gtgcgggcac gagcaccgtc gtttcgggcg gcaagactca aggcgagggt
    20581 ggggctcctt cgcggggcgg aaccagctcc gtgggcggca actctggagg tatcggaagc
    20641 gcgcaaggag gcgcgtggac tggcggtgcg agtcaaggtg gctcgtctca agcgggatct
    20701 tcccccggcg gagcttcggg ctcttgccct tcgaacgccg ccttttgctc cagcttcgaa
    20761 gattccgcga tgccagccgg cgccgtgttc aagctcaacg gcgatccggc ttcgccttgg
    20821 accgtcaact tcgaagtcga cacgacgatg gcgaaatcgg gcaaatcgtc tctgcgcgtg
    20881 aagtctgcca atgagggcgg aggcggcgcc tacaagatgc tcgccgtgcc gacgggtggc
    20941 gctgcgttct gggtgcggat gtacatgcgc agtgacaagg atattggcga gatcgatcac
    21001 aacgtatttg cgcaagccgc aacgaagacc gatcccaacg acgatcctca catggagttc
    21061 gcagaggacg taggactctc tctgcattac aacgacgcag agatcaaatg gccaatgggc
    21121 tatggtcgaa cctcctccgg aggcaccacg ccttacaagt tggtcaagga tacttggcac
    21181 tgtgtggagc tcttcttcga cggccccggc aaaacttaca ggttttacgc caacggcatg
    21241 gaggtgatca gcgcaaacgg gttcccgtcg gcctcccccg catattccac cttcaagttc
    21301 ggatacaact cactgcacgg cacccagcgc aaaacctggt acgacgacgt cgccgtggga
    21361 ccgtcgcgaa tcggctgccc ctaaacacac cgagttcccc gctccgttgc gcggcacggg
    21421 ttgagatctt cggatcgagc gtcgggcgta tcctgcggat tcgcccgacg cgatgccgtg
    21481 catcacatgc ctttcgcggc cggcatgtga gcgggcagag ctgcgggcag ccaacgctcg
    21541 cccgtttcgc agtttatgtg gttgaggtgg agtttaccct cggtcaactg
//
LOCUS       DASTKU010000670         3664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1691081, whole genome shotgun sequence.
ACCESSION   DASTKU010000670 DASTKU010000000
VERSION     DASTKU010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691081"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFQ61_23335"
     CDS             <1..456
                     /locus_tag="VFQ61_23335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HET9957460.1"
                     /translation="STDQAAESDPTPFVKTLTGLIRAEDSYGAWDKKSDTQLLSEFVV
                     TKEERRQIPIIGDPDPDIIHRVEQFYRAVGLRIEQRTGLMASPTVNLHHEGFGRVFLT
                     VGKLVAFAKTLRDVHRFGFEDLLDLTDEGEKAVERAIKTIEQYPEVARA"
     gene            453..650
                     /locus_tag="VFQ61_23340"
     CDS             453..650
                     /locus_tag="VFQ61_23340"
                     /inference="COORDINATES: protein motif:HMM:NF016938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HET9957461.1"
                     /translation="MSDVDALKTELKKLSAKAMQLKMDLHDLSEELPQNWQNILTVAQ
                     QAYDAFAVLERKRGELQKLGS"
     gene            657..950
                     /gene="fdxB"
                     /locus_tag="VFQ61_23345"
     CDS             657..950
                     /gene="fdxB"
                     /locus_tag="VFQ61_23345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin III, nif-specific"
                     /protein_id="HET9957462.1"
                     /translation="MQTRDGRDWQPQYLLKIDAKACIGCGRCYKVCGREVMTLKGIND
                     EGEVVELEEDEDEIEKKVMVMADTGACIGCGACARVCPTNCQTHGLEAMAGAA"
     gene            1030..1641
                     /locus_tag="VFQ61_23350"
     CDS             1030..1641
                     /locus_tag="VFQ61_23350"
                     /inference="COORDINATES: protein motif:HMM:NF016762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifQ"
                     /protein_id="HET9957463.1"
                     /translation="MTGANPSACSGFDAHVLACTLALTAVEGGGDGRALLQGLGLDRA
                     ELLALVGATFPHAAELFARTLPLDVQLDLQADELCLRELLTRGATERSPLQLALAFII
                     ARRCQRPNHLWQDLGLRNRRELSWLMERHFQPLAQRNTRDMKWKKYLYRVICRDEGFA
                     LCTAPSCSECSDFEHCFGGEDGESLLARARRDAELGVASGAAE"
     gene            complement(1702..3207)
                     /locus_tag="VFQ61_23355"
     CDS             complement(1702..3207)
                     /locus_tag="VFQ61_23355"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9957464.1"
                     /translation="MPQVPQLQPLSPQPVEPPPAALARSQSSGSQSAGLQSAGLQSAG
                     AKSAAVYMTRLAGWGANRYSESRYREPETSGQVRAWLRAASVEGVTSISRGLGRSYGD
                     AATNGGGQVLGMTHLSRYLAFDDATGTLTCEAGVSLEQIIQHFTPRGWFPMITPGTKY
                     VTVGGCIANDVHGKAHHAQGSFSTCVESMTILLANGEVVTASRTENPDLFWASFGGMG
                     LLGIVLTATLQLRRIETTYFRQQAFRVRNLEEMLSVLDAQDQQFPYSVATLDVFAKGA
                     ALGRGVITVGDHVSLEQLPTPLRGDPLKGAPTRRLTLPFRLPEFTLNPLSMRVVNAAI
                     QAIQSGARPFGHFEGFFYPLDMISHWNRGYGRRGFTQYQFVIPFQDGVRRMRDILTAI
                     FSAGELPFLNVLKRMGKASASPLSFPAEGYTLAIDFPIRQNTAALCKRLDALVLEAGG
                     RIYLGKDSFLDAATFHAMYPQAKGWLETKAKYDPHGVFTSDLARRVGLTLD"
BASE COUNT          697 a         1078 c         1242 g          647 t
ORIGIN      
        1 tccaccgacc aagccgcgga gtccgacccg actccgttcg tcaagaccct cacgggcttg
       61 attcgcgccg aagacagcta tggtgcttgg gacaagaaga gcgacactca gctgctttcc
      121 gagttcgtgg tgaccaagga agagcgccgt cagatcccga ttatcggaga ccccgatcca
      181 gacatcattc accgcgtcga gcagttctac cgtgcggtgg gtctgcgcat cgaacaacgc
      241 actggtctga tggcgtcgcc gaccgtgaac ctgcaccacg aggggttcgg gcgcgtgttc
      301 ctcaccgtgg gcaagttggt ggctttcgcc aagacgctgc gggacgtaca ccgcttcggg
      361 ttcgaggatc tcttggattt gacggacgag ggcgaaaaag ccgtggagcg cgcgatcaag
      421 accatcgagc agtacccgga ggtcgcgcgc gcatgagcga cgtggacgca ctcaagacgg
      481 agctcaagaa gctctcggcg aaagccatgc agctgaagat ggatctacat gacctatccg
      541 aggagctgcc ccaaaattgg caaaatattc tgacggtcgc ccagcaggcg tatgacgcat
      601 tcgccgtgct ggagcgcaag cgcggtgagc ttcaaaagct cggatcgtga ggcatcatgc
      661 agacgcgaga tggccgtgat tggcaacctc agtatcttct gaaaatcgac gccaaagcct
      721 gcatcggttg tgggcgttgc tacaaggttt gtgggcgtga ggtcatgact ctgaagggca
      781 tcaacgacga aggcgaagtc gttgagctcg aggaggacga ggacgagatc gagaagaagg
      841 tcatggtcat ggccgatacg ggtgcgtgta tcggatgtgg agcctgcgcc cgggtatgtc
      901 ccacgaattg ccaaactcac gggctggagg cgatggccgg cgcagcctga tcgatatcgg
      961 actttcgagg cccattgcgg cgaagaccgg gaggtgctcg tggagcaaat ggatgtctat
     1021 acgtttctca tgaccggtgc gaacccgagc gcgtgctctg gattcgacgc gcatgtgctg
     1081 gcgtgtacgc tcgcgttgac ggcagtggag ggagggggcg atgggcgagc gctgcttcag
     1141 gggctgggct tggaccgggc ggagttgttg gccctggtgg gggcgacctt cccgcacgcg
     1201 gcggagctct tcgcgcgcac gttgccgctg gacgttcagc tggacttgca ggcggacgag
     1261 ctgtgcttgc gtgagctact gacgcgcggg gccaccgagc gtagtccatt gcaattggcg
     1321 ttggccttca tcatcgcgcg gcgttgtcag cggccaaatc acctttggca agacctcggt
     1381 ttacgcaacc ggcgggagct ctcctggctg atggagcgac actttcagcc cctggcgcag
     1441 cgcaatacgc gcgacatgaa gtggaagaag tacctgtacc gcgtgatctg ccgtgacgag
     1501 ggcttcgcac tctgcaccgc tccgagctgc agtgagtgca gcgatttcga acactgcttc
     1561 ggtggcgaag acggagagag tctgctcgct cgagcgcgac gggacgccga gctcggcgtg
     1621 gcgagcggcg cggccgaatg agctcgatcg tctgcgcgtg ggctcgtaga cgtagattca
     1681 cccccacgca agctcgagag ttcaatccag cgtgagcccc acgcggcgcg ccaaatccga
     1741 ggtgaagacg ccgtgcggat cgtacttggc tttcgtttcg agccagccct tggcctgcgg
     1801 atacatggcg tggaacgtgg cggcgtcgag gaacgaatcc ttgccgaggt agatgcggcc
     1861 tccggcctcg agcacgagcg cgtccaaacg cttgcagagc gcggccgtgt tttgccggat
     1921 cgggaagtcg atcgcgagcg tgtagccctc ggccgggaac gatagcgggc tcgcgctggc
     1981 cttacccatc cgcttgagca cgttcaaaaa cggcagctcc cccgcggaga agatggcggt
     2041 caagatatct cgcatgcgcc gcacgccgtc ttgaaacggg atcacgaatt gatactgcgt
     2101 gaagccgcgc cggccgtaac cgcggttcca gtgcgaaatc atgtcgagcg ggtagaagaa
     2161 cccttcgaag tgcccgaagg gtcgcgctcc ggactgaatg gcctgaatgg cggcgttcac
     2221 gactcgcatg ctgagtggat tcagagtgaa ctccggcaag cgaaagggca gcgtcaatcg
     2281 ccgggtcggt gcgcccttga ggggatcgcc gcgcaacggc gtgggcagtt gctccaaact
     2341 gacgtggtcc cctaccgtga tcacgccacg accgagcgcg gcgcccttcg caaacacatc
     2401 gagtgtcgcc accgaatagg ggaattgctg atcctgtgcg tcgagcacgg acagcatctc
     2461 ttccaggttc cgtacgcgaa aagcttgttg acgaaaatac gtagtttcga ttcttcggag
     2521 ttgcagcgtc gccgtaagta cgattccgag gaggcccatg ccaccgaagc ttgcccagaa
     2581 cagatccgga ttctcggtcc gacttgcggt aacgacctca ccgttggcga gcaagatcgt
     2641 catcgactcg acgcaggtgc tgaagctacc ttgggcgtgg tgggccttgc catgcacgtc
     2701 gttggcgatg cagccgccca ccgtgacgta cttggtgccg ggcgtgatca tcgggaacca
     2761 gccgcgcggc gtgaagtgct ggatgatttg ttcgaggctc acgcctgctt cgcacgtcag
     2821 ggtgcccgtc gcgtcgtcga acgcgagata gcggctgaga tgcgtcatgc cgagcacctg
     2881 cccgccaccg ttcgtggccg cgtccccgta gctgcgtccc aggccacgcg agatggacgt
     2941 caccccctcc acgctcgcgg cgcgcagcca tgcgcgaacc tgcccgctcg tctcgggctc
     3001 ccggtaacga gactcggagt aacgattggc gccccaaccc gcgaggcgcg tcatgtacac
     3061 ggcggcgctc ttcgcgcccg cactctgtaa acccgcactc tgtaaacccg cactctgtga
     3121 acccgagctc tgcgagcgcg ccagcgcggc cgggggtggc tcgacgggtt gcggcgagag
     3181 gggctgcaat tgcggtactt gcggcatgtc ctgactttgc atcaccgggt cgtcggcgag
     3241 tttagctgac ccggaccgcc ccgccaggca ctcgtgtttt cacgcggccg cgcgccgcgg
     3301 gagggtggcg gccgcacggc ttgacggccc tccagcgggt gggtgggcgc cacatcacca
     3361 cgagcgcgcc gcccgtcgcg cttcccgcat gcgccgccca atgcgcccag ctcacgtccg
     3421 tggcaccgcg ccggcgccca accgaacgcg cgacgctagc cccgtccatc tcgcgttggg
     3481 aaagcgcgcg tgaggagact tcgaggagag aacgaggaga cccgagagag cgcgggcagg
     3541 ttggaaaggg caggttggaa agggcaggtt ggaaagggca ggttggaaag ggcaggttgg
     3601 aaagggcagg ttggaaaggg ctggttggaa agggcaggtt ggaaagggca ggttggaaag
     3661 ggca
//
LOCUS       DASTKU010000671         5592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_351611, whole genome shotgun sequence.
ACCESSION   DASTKU010000671 DASTKU010000000
VERSION     DASTKU010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351611"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VFQ61_23360"
     CDS             complement(<1..829)
                     /locus_tag="VFQ61_23360"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957465.1"
                     /translation="MRAPKGLRSQRLVLGQGLIWALGLLVHCSVDRKHVFGDISSSRG
                     SSGEAGAPPEGEPGGTTSSGGSAGWGGSSESTGGDLNEGGAKPDDTMLSGGQNSGGRE
                     SVGGTTTSDEDSTGGGVQSGGASGAAGADGEGGSSSGGAPAQGGMTTTAPAHLTVVKL
                     WAAGSGACALMNDDSFICKPEIPQLAGLNPLEIAVPCVVLRDQTARCWGANDVGQVGD
                     GTNVPATNPVPVSGLGNVSQIVSGSYHRCALLNDGSVKCWGEGEFGQLGNGKLYIQSS
                     "
     gene            complement(1027..1386)
                     /locus_tag="VFQ61_23365"
     CDS             complement(1027..1386)
                     /locus_tag="VFQ61_23365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957466.1"
                     /translation="MTKNFKFMGLFAIAGWAALSMMLACGSPVDNDPVPPPSCVHEGK
                     TFGVGERYTQGCGGCQCMEDGSWGFCTGYCPDKIPEVTCERDGVLYPVGTVVSRGCGS
                     CICLASGEFGQCGGACR"
     gene            complement(1653..2342)
                     /locus_tag="VFQ61_23370"
     CDS             complement(1653..2342)
                     /locus_tag="VFQ61_23370"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HET9957467.1"
                     /translation="MKGPLKALELLTLGALSVSCALLTKSEALNARYFSPEGAHQSPD
                     SRHAPMPTTPTSELRLGRVNAAGYLGERIVYRDSKYELNFYDERRWAENPDVYLRRAL
                     GRALFEERGLRRIVAGAGPTLEVELDEFAELRAAPARARVRVTYRLYDNRITRREATL
                     TLEKPIATEVSRDDAVEPKVRAMAEVLNEAVEQIATQVMADLSASPASSVTSDSIGSN
                     RVRAEPAQSER"
     gene            complement(2339..3295)
                     /locus_tag="VFQ61_23375"
     CDS             complement(2339..3295)
                     /locus_tag="VFQ61_23375"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET9957468.1"
                     /translation="MATPSNHWKLGLFVVCCIAVGLAALILLGAHGLNRPTVTYRSYF
                     DESVQGLEIGAPVKFRGVTIGSVAEIGVAPDRRHVMVTSNLGVDQARTMGLAGSESDA
                     PRVPVDLRVQLASAGITGVKFIQIDFFSPITNPVPKLPFEAPKNYIPAAVSTMKNLED
                     AVVLAVSRFPEMTNAAMALIDKLDRLVDDVDRTRLPERTLSTLAAAQQAFAELKTTLT
                     RTEVGKLSEHARSTLGRADELIARVDKLVAKIDGDRGVVMSAQRASDAIGDVAQNAGG
                     LTEDLGATLRDVQQAAEAVQKLADALERDPDMLLKGRARRAP"
     gene            complement(3296..4036)
                     /locus_tag="VFQ61_23380"
     CDS             complement(3296..4036)
                     /locus_tag="VFQ61_23380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9957469.1"
                     /translation="MTEPIIRVTDLTIGHDDVVLQKRANFEVRAGDIFAILGGSGCGK
                     STLLRYLVGLERAETGEIWIAGLGKPSLELGRPPFGVMFQSGALFGSMTVGENVALAL
                     EQWTNLPPDAIAAVVLAKLRLVGLQGAEDKFPAELSGGMKKRAGIARALALEPPLIFL
                     DEPSAGLDPVSAAELDELILTMNRGLGLTVVIVTHELESIFRIANRCIMLDRESQSII
                     ARGDPRELRDQSEDPRVKGFFNRLTREG"
     gene            complement(4050..5216)
                     /locus_tag="VFQ61_23385"
     CDS             complement(4050..5216)
                     /locus_tag="VFQ61_23385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HET9957470.1"
                     /translation="MTEGPGAPERLSIPRLSRIELAASRSALPELILEGQLEFSRAAG
                     LWKSINKRLKAVPRGATLNFDMSRVDSVDGGTMALLVHLRNELKLRGVRSDFVGATGA
                     VQDLVHLYRGDEKPIRKRRRRPKSTLDEIGERTRAFFGSVREVLGFLGDVILTLLGLL
                     REPRTANWKEIPHLMERAGADAVPIIVLINFLVGFVMAFQGAIQLKQFGANIFVADLV
                     GLSVTRELGPLMSAIIVCGRSGAAFAAELGTMKVSEEIDALRTLGFGPIRYLVVPRTV
                     ALVLVLPMLTLLADLVAICGGMVVGIVSLDLSIAGYLSETRQAVHVWDVFSGVLKSSV
                     FALAIALISCQQGFATSGGAEGVGRRTTSSVVSILFSLILIDAVFTVLFYFFHL"
     gene            complement(5322..>5592)
                     /locus_tag="VFQ61_23390"
     CDS             complement(5322..>5592)
                     /locus_tag="VFQ61_23390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957471.1"
                     /translation="RRGRRRHRQDPRKTRALAVALLSVNHILLRLDERHRAWARCRFC
                     VSGVGNDRQSASKHRALREALRDFTHLRGLRSFVHRARLVSALLA"
BASE COUNT         1054 a         1848 c         1652 g         1038 t
ORIGIN      
        1 tcgacgattg tatgtagagt ttcccgttgc ccaactgacc gaattcgcct tcaccccagc
       61 acttcacgga gccatcgttg agcaaggcac agcggtgata actcccagag acaatttggc
      121 tgacgtttcc gaggccgctc accggtacgg ggttcgttgc aggaacattg gtgccgtcgc
      181 cgacctggcc cacgtcgttg gctccccagc agcgtgcggt ttggtctctc agaaccacgc
      241 aaggtacggc gatttcgagc ggattgagcc ccgcgagctg tgggatctcg ggcttacaga
      301 tgaaactgtc atcgttcatc agtgcgcacg ccccagaacc tgccgcccac aacttcacga
      361 cggtcagatg tgctggcgcg gtcgtcgtca ttccgccttg agcgggcgct ccgccgctcg
      421 agctgccgcc ctctccgtca gccccagcag ctccgctcgc gccgcctgac tgcacaccgc
      481 ctcccgtcga gtcttcatcg ctcgtcgtag tgcctccgac ggactcgcga ccacccgagt
      541 tttgaccgcc ggataacatc gtgtcgtcgg gtttggcccc gccttcattg aggtcccccc
      601 cggtggactc gctggaccca ccccagcccg cgctgccgcc ggagctagtc gtgccgccag
      661 gctcgccctc gggaggggct ccggcttcac cgctactgcc gcgagacgag gatatgtccc
      721 cgaacacgtg tttccgatcc acgctgcagt ggaccagaag acccaatgcc cagatgagcc
      781 cctgacctag aacgagcctt tgcgaacgga gacctttggg tgcgcgcatg gtgacctgag
      841 agcgatgtct gctctcccga tggcgcgggc ttggcggccc tatccccgcg cgagagctag
      901 aaccgccgat cctacgcgag ccacccccgg gaagaatcgc cgagcgcgtg gtcatttttg
      961 cagtcagcgt cgctcgacga gcgaccggaa gctcgaatct ctacttccgt ttacttcgcg
     1021 cgagcttcag cggcacgctc ccccgcactg cccgaactca ccgctagcca ggcaaataca
     1081 gctgccgcac ccgcgagaca cgaccgtgcc taccggatac aacacgccat ctctctcgca
     1141 ggtgacttcc ggtattttat ccggacaata cccggtacaa aatccccagc tcccgtcttc
     1201 catgcactga catcccccac atccttgggt gtaacgctct ccgaccccga aggtcttccc
     1261 ctcatgcacg caactcggcg ggggtacagg atcattgtcc actgggctgc cgcaagccaa
     1321 catcatagag agcgccgccc aacccgcgat cgcaaacaac cccatgaatt tgaaattctt
     1381 cgtcacgttc cgtcctccac aagcgattac taggcgatgc atacggacga gcgcagcggc
     1441 actactggcg cgagcttact gaacaagcag ccctcgatgc cgaaggcacc cgcatgcatg
     1501 ggtaccattc agcaatcccc gtgccttccg ctttagtcgg cacttgccac aaactctcgc
     1561 tgaaatgggc tgtgccagcc tgtgtcagtt cgcgccgcgg ttctctcgag ggcacagcct
     1621 ctcgatcgca gggcattctc aaacgcttcg ctttaccgtt ccgattgcgc tggctctgcg
     1681 cgcactcggt tcgagcctat agagtctgac gtcaccgagc tagctggaga ggccgagaga
     1741 tcagccatca cctgcgtggc gatctgctcg acggcctcgt tcaacacttc ggccatggcg
     1801 cgtactttcg gctcgaccgc atcatctcgg ctgacctcgg ttgcaatcgg cttttcgaga
     1861 gtgagcgtgg cctcgcgtcg cgtgattcga ttgtcgtaga gtcggtaagt cacgcgtacg
     1921 cgggcgcgag caggcgcggc acgtagctcc gcgaactcgt cgagttcgac ttctagcgtc
     1981 ggccctgcgc cggccacgat acggcgcaac ccccgctctt cgaatagggc tcgcccgagc
     2041 gcgcgtcgca gatacacatc agggttctcg gcccagcgtc gctcgtcgta gaaattgagc
     2101 tcgtacttcg aatctcgata gacgatgcgt tctcccaagt agcccgccgc gttcacgcgc
     2161 ccgagtcgaa gctcgctcgt cggcgtggtc ggcatcggcg cgtgacgtga atcagggctt
     2221 tggtgcgcac cctcggggct gaagtagcga gcgttcagcg cctcgctttt ggtgagtagc
     2281 gcacagctca cgctcaatgc acctagcgtg agcagctcga gtgctttcag gggacctttc
     2341 aaggtgcccg cctcgcgcgc cctttgagca gcatgtcggg gtctcgctcg agcgcgtccg
     2401 ccaacttttg cacggcttct gcagcttgtt gcacgtcacg cagggttgca cccaagtcct
     2461 cggtgagccc ccccgcattc tgtgcgacgt cgccgatggc atcggaggcg cgctgcgcgc
     2521 tcattaccac tcctcggtct ccgtctatct tggctactag cttgtccact cgcgcgatca
     2581 gctcgtcggc tcgacccaac gtcgaacgtg cgtgctccga cagctttccc acctccgtgc
     2641 gcgtcagtgt ggtcttgagt tcggcgaaag cctgctgggc cgcggcgaga gtgctcagcg
     2701 tacgttcggg cagtcgggtg cgatccacgt catccaccaa gcgatcgagt ttgtcgatca
     2761 gagccatcgc ggcgttggtc atctcgggga agcggctcac cgcgagcacc accgcatcct
     2821 ccaggttctt catggtcgag accgcagccg ggatgtagtt tttgggtgct tcgaacggca
     2881 gcttggggac cggattggtg atcggcgaaa agaagtcgat ctgaatgaac ttcacgcccg
     2941 tgatgccggc ggacgccagc tggacccgca agtcgacggg tacgcgcggg gcatcgctct
     3001 cgctccccgc gaggcccatc gtgcgcgctt ggtcgacgcc gaggttgctc gtgaccatca
     3061 cgtgtcgacg atccggggcg acaccgatct ctgcgacgga gccgatcgtc acaccgcgga
     3121 acttgaccgg cgcgccgatc tcgagaccct gcacggactc gtcgaagtaa gagcgataag
     3181 tcacggtcgg ccgattgagc ccgtgcgctc cgagcaaaat caaagccgcc agccccacgg
     3241 cgatgcagca caccacgaat agaccgagct tccagtgatt gctgggggtt gccactcagc
     3301 cctcccgagt tagacggttg aagaagcctt tgacgcgcgg gtcctcgctc tgatcgcgaa
     3361 gttcacgcgg atccccacgg gcgatgatgc tctgactctc tcgatcgagc atgatgcagc
     3421 ggttggcgat cctgaagatg ctctcgagct cgtgggtcac gatcacgacc gtcagcccga
     3481 gccctcgatt catggtcaaa atcagctcgt cgagctcggc tgcgctgacg ggatccaggc
     3541 ccgccgacgg ttcatccagg aagatcaacg gcggctcgag cgcgagcgcc cgcgcaattc
     3601 ccgcgcgctt cttcataccg cccgagagct ccgcggggaa cttgtcttcc gcgccctgca
     3661 gccccaccag ccgaagcttg gccagcacga ccgcggcgat tgcatcgggc gggaggttcg
     3721 tccactgctc cagagcaagc gcgacgttct cgcccaccgt catggagcca aatagcgcac
     3781 cggactgaaa catcactccg aagggtggtc gaccgagctc caagctcggc ttgccgagcc
     3841 ccgcaatcca gatctccccc gtctcggccc tttcgagccc caccaagtac cggagcaggg
     3901 tggatttgcc gcagccgctg ccgcccaaaa tggcgaagat gtcgccggct cgaacctcga
     3961 agttggcgcg tttctgcaat acgacgtcgt cgtgaccaat cgtcaggtcc gtaactcgga
     4021 tgatcggctc ggtcattgcg acgctcattt caaaggtgga agaagtagaa gagcacggtg
     4081 aagaccgcgt cgatcaggat gagtgaaaac aggatcgaga ctacggacga ggtggtgcga
     4141 cgccccacgc cctctgcgcc gccgctggtc gcaaagccct gctggcaaga gatgagcgca
     4201 atcgccaaag cgaatacgct gctcttcaac acgccagaga acacgtccca gacgtggacg
     4261 gcctggcgcg tttcactcaa gtagccggcg atcgagagat cgaggctcac gatgccgacc
     4321 accattccgc cgcagatggc gacgagatcg gcgagcaggg tcagcattgg taacaccaga
     4381 accaatgcaa ccgtacgggg cacgaccaag tagcggattg ggccaaaacc cagcgtgcgt
     4441 agcgcgtcga tctcttcgga gaccttcatc gtgccgagct cggccgcgaa ggcggcgccc
     4501 gaacgtccgc acacgataat cgcgctcatc aacggcccga gctccctcgt tacggagagc
     4561 ccgaccagat ccgcgacgaa gatgttggcg ccgaactgtt tgagttggat ggccccttga
     4621 aatgccatca cgaagccgac caagaagttg atcagcacga tgatgggcac cgcgtcggct
     4681 ccggcgcgct ccatcaagtg gggaatctct ttccaattcg cggtgcgcgg ttcccgcagc
     4741 agcccgagca aagtcaagat gacgtcaccc aagaagccga gcacttcacg aaccgaaccg
     4801 aagaaggcac gggtgcgttc cccgatctcg tccaacgtgc ttttgggtcg ccgacgacgc
     4861 ttgcggatgg gcttttcgtc tccgcggtag aggtgcacta ggtcttgcac cgcgccggtc
     4921 gcgcccacga aatccgatcg taccccgcgc agcttgagct cgttccgcag gtggacgagc
     4981 aacgccatcg ttccgccgtc cacgctatcc acgcgcgaca tgtcgaagtt cagggtggcg
     5041 ccgcgcggga ccgctttcag tcgcttgttg atgctcttcc aaagccccgc ggcgcgtgaa
     5101 aactcgagtt gcccttccaa gatgagctcg ggaagcgcgg agcgcgacgc ggccagctcg
     5161 atgcgagata gacgtgggat ggagagccgc tcgggcgcgc cgggaccctc ggtcataggc
     5221 gatcgggctc catggaagtg atctcgagtc gcacgcgaac gcgaccgctc tacgctactc
     5281 tagcgcaaga gtaggagcgc ggtacgtgtg tagaatcacg ctcacgcaag tagggcgctc
     5341 acgagccgcg ctcgatgaac gaagctacgt agtccccgga ggtgcgtgaa atcgcgcagc
     5401 gcttcccgca atgcgcgatg cttggacgcg gactgccgat cgttgccgac ccctgaaaca
     5461 cagaagcggc accgagccca agcccggtgc cgctcgtcga gccgcagtaa gatgtgattt
     5521 acagagagta gagcgacggc aagagcccgc gttttacgag gatcttgacg atggcgtcgg
     5581 cggcctcttc tg
//
LOCUS       DASTKU010000672         4283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_129745, whole genome shotgun sequence.
ACCESSION   DASTKU010000672 DASTKU010000000
VERSION     DASTKU010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129745"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFQ61_23395"
     CDS             <1..194
                     /locus_tag="VFQ61_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3079 domain-containing protein"
                     /protein_id="HET9957472.1"
                     /translation="PPHPERVCWGCDRLCAANDLGCGDDTVRTPHPCELFGDDWVQWA
                     EENGAASPMYRLFANHQEE"
     gene            462..2357
                     /locus_tag="VFQ61_23400"
     CDS             462..2357
                     /locus_tag="VFQ61_23400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957473.1"
                     /translation="MLRPKSRVAAVLVTAAVAPLLVSASAHASSIELFAQVPYEFETG
                     EFDLNDLEWQNTEPLFAQNLHIPYATLETQGRAQIAQLTKPLTGHEPCGDGILDPLCP
                     DADWTVTFTADFRFTQHADPVLTAYGTPAENGVRAHLETELSVQVNAHVHVETSIQDA
                     DFDVPIDALIGVSATTAFKLYPVLQADELDLKLTWRGGNVELEGLDGQAIADGAVLGT
                     ALGFTPLGAVFGGPLGGGILGAVTGQVALDAAKEKAKGIIEQKAKAALESAGTLVTNE
                     LGARLRPKLQQANNVLASIRAQPVPGTGLTLGQLESALGMSMDVRSVTVDDVFRSAIT
                     TRFANSPGAGQLQGLVVFPRTACNYQQVRSKLLGNLLVALDLKPINQDLVGKRCSEAA
                     GELAHRAYYGENPDRVLKSGAAQNQLATWATFGALELPGDVVELPDALVCSYGLSGLP
                     NTAIFEVTPERGSALTERLSSNRLGFAPAAGSLIDQMLENSRQFGQRLLLLDQGGLPA
                     AFDHEGTPLDPMALDFGGPGPASPADCPTVVLASGTGLFTSPLDELENARDPEKCPYC
                     VAPSLGFEEHVRWEGVSAVSPQTNRPDVRGNLELRDTLTYSDSALVTGSAVTATVPGA
                     RLPQLRR"
     gene            2485..3561
                     /locus_tag="VFQ61_23405"
     CDS             2485..3561
                     /locus_tag="VFQ61_23405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957474.1"
                     /translation="MAAPNEAAPSSNTKPAGEPRVAEYAAAHSEGEEQEAEPACPVPQ
                     GVIADNARLLADEFFARGKALWTSGKLANACECLQNSDRLAPGRGGTMLALGLCREEQ
                     GRLGLAYRELQNARAKAREDGRSDREELAVQHLRALEPRLAWLTVVSRRLEGAAEGRF
                     WLDGMPIRIEGPALPLELGTHVLKVELPGFVTQELSVELTQPGERRTLEIPSLERRAA
                     ASKPGGEGTTSHSVPSSGNPERARAARVEPRRAAASPTSSPASSTLAQLKPATLALGI
                     VGVAVSLGAGAWALERKATVHAECDAAKQCTDKGLSAVSTGRTLIVVGTTAFAVGAVS
                     LGAWVLLPSSGTSNSARVVLSGVF"
     gene            3563..>4283
                     /locus_tag="VFQ61_23410"
     CDS             3563..>4283
                     /locus_tag="VFQ61_23410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957475.1"
                     /translation="MRASSWSVLGFLVGGTLSGCGVLVGESFEGYSHQRSSSSGGEAG
                     LAGELSSPGGSVDSGGASSGGFMGTEPPSCPTTEYLITDMEELNTADPSYAKVSPLGV
                     GSGIEAYGFYCGHGDSGAPCTPDASGFEPTLLPEEAHCGAQALKAYGRGTEKLGISLD
                     LTMSFEHALNYPGVSVWAKGAGPFVPGLTQGGDWYDLPELDPEVWQEYVLRWSDLKDE
                     NRRPWKFDGKLGAADDGAGHKL"
BASE COUNT          780 a         1273 c         1455 g          775 t
ORIGIN      
        1 ctcccccgca ccccgaacgc gtctgctggg gatgtgaccg tctgtgcgcg gcgaacgatc
       61 tgggctgcgg cgacgacacc gtacgcacgc ctcacccttg cgagctgttt ggggacgact
      121 gggttcagtg ggcggaagag aacggcgcgg caagcccgat gtatcggctc ttcgccaacc
      181 atcaagaaga gtgagcctgc gtcgtgcctt gcgctccagc ggagatgcgg gggccggcgt
      241 ggtggtgagg gaatcctggg gaggctgaga ctgcgccaga ggctgaccgt gacgggcttc
      301 gcgcacgttg cagagcggcc cgatctccct gaaatggaag cgcgctagta agtgcgttgc
      361 cccgagtgag aatcgaagcg tggattgtgc gactcggggg cgcgagcggc atccccctgt
      421 cgtgacgccg gtcgagtggc gccgcaggaa aggaattgcc catgcttcgt ccgaaatcga
      481 gggtcgcagc cgtcctggtc actgccgccg tggcaccgct actggttagc gcatccgcgc
      541 acgcctcgtc gatcgagctg ttcgctcaag ttccttacga gttcgagacg ggcgagttcg
      601 atctgaatga cctcgagtgg caaaacacgg agccgctgtt cgcccagaat ctgcatattc
      661 cttatgcaac tctggaaacg caaggcaggg ctcagattgc gcagctgacc aagccgctca
      721 ccggtcacga gccgtgcgga gatggaatcc tggatccact ctgcccagat gcggattgga
      781 cggtgacgtt cacggctgac ttccggttca cgcaacacgc cgatccggtg ctgacggcgt
      841 acggcacacc ggcggaaaat ggtgttcgcg cacacctgga aaccgagctg agcgtgcagg
      901 tgaatgctca cgtgcacgtg gagacgtcga ttcaggacgc agacttcgac gttccgattg
      961 atgccttgat tggagtatcc gccacgaccg cgttcaagct ctaccccgtg ctccaagcgg
     1021 atgagctgga tttgaagctg acttggcgcg gcgggaacgt agagctcgag ggcctcgatg
     1081 gccaagccat cgcggatggg gcggtgctcg ggaccgcgct gggcttcacg ccactcggtg
     1141 ccgtgttcgg agggccgctg ggcggcggga ttctgggcgc ggtcacgggt caggtggcgc
     1201 tcgacgccgc gaaagagaag gcaaagggca tcatcgagca gaaggcgaag gcggcgctgg
     1261 agtcagccgg gaccctggtt acgaatgagc tgggggcgcg gctgagaccg aagttgcaac
     1321 aagccaacaa cgtgctcgcg tcgattcggg cccagcccgt gccgggcacg gggctgacgt
     1381 tgggccaact agaaagcgct ctgggcatga gtatggatgt gcgcagcgta accgtcgacg
     1441 atgtgtttcg ctccgcgatc accacgcgct tcgcgaactc accgggtgcg ggccagctgc
     1501 aagggctggt ggtctttccc agaacggcct gcaattacca gcaagtcagg agcaaattgc
     1561 tcgggaatct gctggtggcg ctggatctaa agccgatcaa tcaagatctg gtgggcaaac
     1621 gctgttcgga ggcggcggga gagctcgcgc atagagccta ttacggtgag aacccagacc
     1681 gggtgctgaa gtccggcgca gcacagaatc agctcgcgac gtgggcgaca ttcggtgcgc
     1741 tggagctccc gggtgatgtg gtggagttgc cagacgcgct ggtctgctcc tatggcctct
     1801 cggggctacc caataccgcc atcttcgagg ttacgcccga acgaggatcc gccttgacag
     1861 agcggctatc gagtaatcgg ctcgggttcg cgcccgcagc gggctcgctc atcgatcaga
     1921 tgctcgagaa tagccgccag ttcgggcaac ggctcttgtt gctcgatcaa ggtggattgc
     1981 ccgcggcttt cgatcacgag ggcactccgc tcgaccccat ggccctcgac ttcggcggcc
     2041 ctggcccggc gagcccggct gattgcccta cggtggtgtt ggcgagcggg acgggtctat
     2101 tcacgtcccc gctcgacgag ctcgagaacg ctcgcgaccc ggagaagtgc ccctattgcg
     2161 tcgcacccag cctaggattc gaggagcacg ttcgttggga gggagtctcg gctgtgtccc
     2221 cgcaaaccaa tcgcccagac gtacggggca atttggagtt acgtgacacg ctgacttaca
     2281 gcgactctgc tctggttacg ggttcagcgg tcacggccac ggttcccgga gcgcgccttc
     2341 cccagcttcg acgataaaca cccttccctt gcggacgtct aagccatctc ctacggtgct
     2401 tcggtgcgcg gccgcttcgc ttctcccgtt gttgccattt ggctcgtccg tgggctcgcg
     2461 ctcttcacga tctgctcgcc cgcgatggct gcgcccaacg aagccgcacc aagcagcaac
     2521 actaaacctg cgggcgaacc ccgcgtcgca gagtacgccg cagcacacag cgaaggtgag
     2581 gagcaagaag cggagcccgc ttgtcccgtt ccgcaaggtg tcattgcaga caatgcgcga
     2641 ctgctcgcgg atgagttttt tgcgcgaggc aaagccctct ggaccagcgg aaagttggcg
     2701 aatgcctgcg aatgcctgca gaacagtgat cgactcgcac cgggccgagg tggaaccatg
     2761 ttggccttgg gcctgtgccg agaagaacag ggtcgactcg ggctggcgta tcgcgagctg
     2821 cagaacgcgc gagccaaggc gcgggaagac ggtagaagcg atcgcgaaga gctcgccgta
     2881 caacacctgc gtgccctcga acctcggctc gcctggctca ccgtcgtttc gaggcgcctg
     2941 gaaggtgcgg ccgagggtcg cttttggttg gacggaatgc cgatccgtat cgaggggccc
     3001 gcactgccgc tcgagctggg aacgcacgtg ctcaaagtcg agctcccggg gttcgtgacg
     3061 caggagctga gcgtcgaatt gacgcaaccg ggagagcgcc gcacgctcga gattccgagt
     3121 ctcgagcgtc gtgcggcagc ttcgaaacct ggcggagagg gcacgacgtc gcattcggtg
     3181 ccgagctcgg gcaatccaga gcgagcgcgc gccgcgcgag tcgagccgcg cagggccgca
     3241 gcttcgccta cgagttcacc cgccagctct acgctagccc agctgaagcc cgcaacgctc
     3301 gcgctcggta tcgttggggt tgccgtgagc ttgggcgcgg gggcttgggc gctcgagcgc
     3361 aaggcgacgg tgcacgccga gtgcgatgca gccaagcagt gtacggacaa ggggctgtcc
     3421 gccgtctcga ccggacgcac gttgatcgtc gtcggaacga cggcgttcgc ggtcggcgcg
     3481 gtgagcctcg gagcgtgggt cttgttgccg agttccggaa cctccaactc ggcgcgcgtc
     3541 gtcctaagtg gagtgttcta aaatgcgcgc ttcgagttgg tcggttctgg gctttctcgt
     3601 gggcggcact ctgagtggct gcggggtgct agtaggcgag tctttcgaag gctattctca
     3661 tcaacggtcc agttcgagcg gaggtgaagc ggggttagcg ggggaactct cgagtccggg
     3721 gggttccgtt gactctggcg gtgcgagctc tggcggcttc atgggcaccg aacccccgag
     3781 ttgcccgacg acggagtacc tgatcaccga tatggaagag ctgaataccg ccgatccgag
     3841 ctacgccaaa gtatccccgt tgggcgtcgg cagcggaatc gaagcctatg gcttctattg
     3901 cggacatggc gattccggag ctccttgcac tccggacgcg agtggcttcg aaccgactct
     3961 gctacccgaa gaggcgcact gcggggcgca ggctctgaaa gcctacggtc gcggcacgga
     4021 gaagctcggc atcagcttgg atttgacaat gtcattcgag cacgcgctga actaccccgg
     4081 cgtaagcgtc tgggcgaaag gagcagggcc cttcgtgccg ggactcaccc agggcggtga
     4141 ttggtacgac ttgcccgagc tcgatccgga ggtttggcaa gaatacgtct tgcgttggtc
     4201 ggacttgaaa gacgagaatc gccgaccttg gaagttcgat ggcaagctcg gggccgccga
     4261 cgacggcgct gggcacaaac tca
//
LOCUS       DASTKU010000673         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_797915, whole genome shotgun sequence.
ACCESSION   DASTKU010000673 DASTKU010000000
VERSION     DASTKU010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797915"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="VFQ61_23415"
     CDS             <1..1532
                     /locus_tag="VFQ61_23415"
                     /inference="COORDINATES: protein motif:HMM:NF015387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HET9957476.1"
                     /translation="GGAGPLSDPDYSQWAEGPTLTKRGEWYYYFVQTHTACSGKSSVW
                     ASKTLDGGETNWTWLGYVMGPGDPYNGTQHPSSPFQIADGTWWAFAHSYDCTGKGSTA
                     EQDGEWLGLGREGLLHQVTWEDTDVNGQTIPVPHFTEGTRNLPAPQLEPSGTPFLLPV
                     NDDFSASKLGTAWTSYGRTGVNLSVTDREGWLRIKPPTGTTVWALQKEALRSTASLTK
                     VEFTPTAEGEAAGISVRNGFSADDQLLSGPTWLEGEGLLVGLFDVSLARTFVGGADMI
                     KFSFRSRVPTPTGGSGNYTSLQDPVEVSYTAPAPQQTAVWLKLVRSNHQATGWFSTDR
                     VTWKQVGQAIDIEELDDNYGMSSAWVGNHAGLFATGKPADFDLFTYRDGFSVIPATAT
                     DQQSGTAIVTSGDKGGVLGQLENGDWAMYGSVDLGTGAKSARIEASSVGGAQLEIWLD
                     PLAGGKRLGSCTVPDTGSWDKWQAIECAVTASGSHDVYLKVVGDAGKELARVSSLQFV
                     Q"
     gene            complement(1851..2588)
                     /locus_tag="VFQ61_23420"
     CDS             complement(1851..2588)
                     /locus_tag="VFQ61_23420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957477.1"
                     /translation="MSNHYHAIVHDPLGTLPVFLEHLHKLAARALNARWGRWENLWAT
                     EQTCATHLVHPGDVLDKLLYTLCNPVAEHLVDRVTDWPGANSYRAMLTGKTIEVERPR
                     DFFRANGSTPDVVRLELVRPPGFEECDPTQWQNHLSGLVSAVEARTRTERLRRGIKLR
                     GRKAVLRASAFETPLGSEPRRDLRPAVACADRATRIEVLARLVDFRRAHEAARLRLAA
                     GDLEATFPYGTYRMRLMGHRCAPAPFN"
BASE COUNT          588 a          931 c         1027 g          542 t
ORIGIN      
        1 agggcggagc cggaccgctc tcggaccccg actatagtca gtgggcggaa gggcccaccc
       61 tgaccaagcg cggagagtgg tattactact tcgttcagac tcacaccgca tgcagcggga
      121 aatcgagcgt ttgggcctcg aagacgctgg acggtggaga gacgaactgg acctggctcg
      181 gctacgtcat gggcccgggc gacccgtaca atggcactca gcatccgagt tcgcccttcc
      241 aaatcgcgga cggcacttgg tgggcattcg cgcactcgta cgattgcacc ggcaagggga
      301 gcacggccga gcaggacggt gaatggttag gcctcggccg tgaagggctc cttcaccaag
      361 tcacctggga ggatacggac gtcaacggcc agacgatccc ggtccctcac ttcaccgagg
      421 gaactcgcaa tctgccggcg ccgcagctcg aaccaagcgg aactccgttc ctgctcccgg
      481 tgaacgacga cttctctgcg tccaagctcg ggaccgcttg gaccagctat ggccgcaccg
      541 gcgtaaacct ctcggtcacg gatcgcgagg gctggttgcg tatcaagcct ccaaccggaa
      601 ccacggtctg ggcgctccaa aaggaggcgc tgcgttccac ggcgtcgctc accaaagtcg
      661 agttcacgcc gacggccgag ggtgaagcgg cggggatcag cgtgcgtaac ggcttctcgg
      721 cggatgatca actcctgagc ggtccgacct ggttagaggg agaaggactg ctcgttgggt
      781 tattcgatgt ctctttggca cgcacctttg ttggcggcgc tgacatgatt aagttctcct
      841 tccgatcgcg cgtccccacg ccgacgggcg ggagcggcaa ctatacgtca cttcaggatc
      901 ccgtagaggt tagctacacc gcgccggcac cgcagcaaac ggcggtttgg ctcaagctcg
      961 tgcgcagcaa tcaccaggcg accggatggt tcagcaccga tcgcgtcacg tggaagcaag
     1021 ttggtcaggc catcgacatc gaggaactgg atgacaacta cggcatgtcc agtgcctggg
     1081 tcggaaatca tgccggccta ttcgcgacgg gcaagcccgc tgatttcgat ctcttcacgt
     1141 accgtgatgg ttttagtgtc atcccagcga ctgcgaccga tcagcaatcg ggaactgcga
     1201 tcgtcacgag cggcgacaag ggcggagtgc tggggcaact cgagaacggc gattgggcga
     1261 tgtacggcag cgtcgatctc gggactggcg cgaagtcggc gcgaatcgag gcatcgagcg
     1321 tcggcggagc gcagctcgag atttggctcg atccgctggc gggaggcaag cgcttgggct
     1381 cttgcaccgt gcccgacacg ggtagctggg acaagtggca agccattgaa tgtgcggtga
     1441 ccgcgagcgg cagtcacgac gtttacctca aggtagtcgg agacgcgggc aaggagttgg
     1501 cccgcgtgag ctctctgcag ttcgtgcagt agagcccacg atcagggatc gcaaatggta
     1561 acggcggggg ggctctgatc ccgcgacgtt ggggcgggac gacgttgcgg gatcggaccg
     1621 ttgcgacgtc gcgagcgtcg gggcgggacg acgttagggc gcggggcggt cgttggtggc
     1681 ggactttcgg gggggctttg tccgggtacg ggcggtcgaa tggggcgacg cgcgaacact
     1741 gcgacgtcag atcgcggata caccgctccc cgtgcgcgtt ggagcgccac ggcggacgta
     1801 gttgtcccta gagccgccgt gggcggcctt gggcagggga cacgggacgg ctagttgaac
     1861 ggcgccggcg cgcagcggtg gcccatcaaa cgcattcgat aggtcccata tgggaacgtc
     1921 gcctctaggt cgccagccgc aagtcgaagc ctggccgcct cgtgcgcgcg ccggaaatca
     1981 accagccgcg cgagcacctc gatgcgcgtc gcccgatctg cacacgcgac agcaggccga
     2041 agatcgcgcc gcggttccga accaagtggg gtctcgaagg ccgaagcccg gagcacagct
     2101 ttgcgtccgc ggagcttgat tccgcgccga agtcgctccg tccgggtgcg cgcctcgacg
     2161 gccgacacca atccgctcaa atgattctgc cactgcgttg ggtcgcactc ttcaaacccg
     2221 ggtggcctga cgagttccaa ccgtacaacg tcaggcgtac tgccgttggc tcgaaagaaa
     2281 tcgcgcggac gctcgacttc aatagtcttt cccgtcagca tggcccggta agagttcgct
     2341 ccaggccagt ccgtcacccg atccacgagg tgctcggcga ctggattgca gagagtgtac
     2401 agcaacttgt cgagcacatc gcccggatgg accaggtgcg tcgcgcaggt ctgctcggta
     2461 gcccacaagt tttcccagcg tccccagcgc gcgttcagcg cgcgtgccgc caacttatgc
     2521 aggtgctcga ggaacacggg taatgttccg agtgggtcgt gcacgatggc atggtaatgg
     2581 ttgctcatcg ccaaaaagcc tacaatccgc actccggcgt attgcgcggc cagcactaag
     2641 caaaacagga aacactggtt cgtccgctcg tcggggcgaa gcaaaaactg ccgtcgcgtg
     2701 cagcgacgag taatcatcca gtagcggccg ggtatgattt cgcggggagc gcacaaagcg
     2761 tatgcccata aggcgagcgc tgtaccatgc ggatatgccc gcagtccccg cgtgcgcccc
     2821 ggtggccgcc agtggcgaag gttggcgaat cgtcgcgcgt ccttgagtac ggggcccggc
     2881 gcggcggagc gtgtcagtaa gtggcacctc gtgcttgcag gatgtcgtca ctttccatcg
     2941 gatttcgctg cccgtgcccg tgtgattttg acggaatacc aaaccttacg acggcgccac
     3001 ggtctgtgcg ggaagcctga aaagccgaaa gagaacttcg ctcggctcag gcgtctgctc
     3061 ggtatcgctg ccgataatcc ggccgcac
//
LOCUS       DASTKU010000674         8774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1469437, whole genome shotgun sequence.
ACCESSION   DASTKU010000674 DASTKU010000000
VERSION     DASTKU010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469437"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFQ61_23425"
     CDS             complement(<1..354)
                     /locus_tag="VFQ61_23425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957478.1"
                     /translation="MRKFGFPLVATTVSALVWASRAQGQERQLTGAGEIQVMGERPTS
                     PRGAGDLRIDRQQLEASPRQHASELLAAAPGFFVDHEDGEGLGNDVTLRGFDLEHGTG
                     IELRLGSVPINSPIHI"
     gene            complement(405..800)
                     /locus_tag="VFQ61_23430"
     CDS             complement(405..800)
                     /locus_tag="VFQ61_23430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957479.1"
                     /translation="MLGACSSGETKGACELGDLDGIIGGDYQFEVTVNDVEFSPKILK
                     AQNSANVRLKLTNSGTVAHNFLLACQPTPNDDGCPQQACFEDEAQVMHLEPGESVTIE
                     FAVPRAEGLYEFKSTLPGPEPLTGQFIVS"
     gene            complement(978..1580)
                     /locus_tag="VFQ61_23435"
     CDS             complement(978..1580)
                     /locus_tag="VFQ61_23435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9957480.1"
                     /translation="MTARILLVEDEPDTRELLLRALKRAGYDCVAAEGPARALLEARR
                     FGALDLVITDVVMGRDDRAGLHLMNDLRTSGFIAPVVIMTAFADVDKVKTALNQGAAY
                     FVEKPFSAAALVEVVQRVLVQRGPEPHVLDDAGLTPKELRVARYLLQGLSSTEIAERE
                     GNSPKTIRQHVTEVYSKCGVAHRAQFFDFVYRGVRTRKPE"
     gene            complement(1577..2569)
                     /locus_tag="VFQ61_23440"
     CDS             complement(1577..2569)
                     /locus_tag="VFQ61_23440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9957481.1"
                     /translation="MKTSSNARLYSYLVPGLLLGSAYVAFDVIAEKRLALGTLTGAFA
                     TLHNWVDHLLPVFAGGLFGLALYQLGLQRRLREAEKAAERAEALKIRLQKVERDQAVW
                     VLIAAVLHEINNPLHALGLLLDELALEDVTPVERSELAQRARAQVQRALGQVGTLRSL
                     RKVGQPELARLDLVSAVRALVNDLEHLGAEQHVQMEVTAEGACFARADSMYLRTILEN
                     LVDNSLHALHARAAGRISIRIYESDGRAVMTVTDDGPAIATDTEAVLFDPLGTSKGPH
                     LGGGAGVGGLASKSTGLGLGLSIARALARAMDGDLVFEREPKCFRLTLPVGVTQ"
     gene            3116..4606
                     /locus_tag="VFQ61_23445"
     CDS             3116..4606
                     /locus_tag="VFQ61_23445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957482.1"
                     /translation="MGMFIQADDGTGSFFPAEVGTHLLTITNFDTGYGEAPPSGELVA
                     VSYARSNSGPPPERLDDLVNNAAYTNDTRIRGVDNNFNVPGVGLISNFAGVAIEVGDA
                     NNPFPNTQLTYYDVTENGGAGYPACDTSNNPIPSPGPIVLYHELSHIHDYHNNVATTE
                     FDAMTDENELRTQMGTEFGQRDPNNHGGCGTATTTCCVVASIATGWPRSPQLHELRKV
                     RDRLLRRSAVGFEFFEALFRDYYYFSPEACRLLGQNSQLSRLVRDRLVDPLVIALRLV
                     DSWRPTPPAPSGAALQFERERSAHPALAALSVAELEWSVAALRSVATGATDSRLEELG
                     MPAEVVALARRVPRSAAVMWAFVEPITLTYSALLEHAKSALLDSAGLDRRDGSQQEQL
                     ESGHRERGQRLLAAFEGWSAGLPLTPGWQRWTCLEIEQQLRFLSEVLLLTSRSRARFR
                     TRMLGELEAGPEVPAGSARPAADDPRLLSSLERWGYFGGEYDAPCH"
     gene            4587..5093
                     /locus_tag="VFQ61_23450"
     CDS             4587..5093
                     /locus_tag="VFQ61_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957483.1"
                     /translation="MTRLAIEEFRMRDNGERLEFSLRIRNDEDRTLHMIAAPRGYEWD
                     AATRRFTVKLHDTPLDPASRVQVALFVPPTFKELEPGRVTEVSFTVPRVLKTADASGA
                     VVVWPVWNALEVRVAFAVSDTPLYPVVDEARRASGLAAPLQLAAASAAWGERVEISAP
                     ARASLETH"
     gene            5130..5696
                     /locus_tag="VFQ61_23455"
     CDS             5130..5696
                     /locus_tag="VFQ61_23455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957484.1"
                     /translation="MATLAEIANQINTTLSQISSNTQDTATTAGLIKGDTADIKTRLD
                     NIRATLQAGFTGLGLGLFACLEQLKKGNSLLELNAAQNETIICWLGKLADLECRQLRV
                     AEHMLETTRAIALDARKVAQITEHVHADAALTVAHENRLEERIRQCCDAPPKDPGPCF
                     EPCRQREVDIYEPRGDWQPPRPNGGEVG"
     gene            5693..6190
                     /locus_tag="VFQ61_23460"
     CDS             5693..6190
                     /locus_tag="VFQ61_23460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957485.1"
                     /translation="MTSRRGAEHHRNGARGWTVLAATALAATWLAASLSTGCKCDPEK
                     VEVPPDDCASRPAAASVTIRASLSGIRPIGSDKQLKINGNREPGQSSCFTGDTNVLTT
                     LTGQSEVSEAHALAHGAWKFTVTPLSGGDHAAVPEQLRTLAPGSSQTLRITSTSSGDI
                     AIGWE"
     gene            6578..7720
                     /locus_tag="VFQ61_23465"
     CDS             6578..7720
                     /locus_tag="VFQ61_23465"
                     /inference="COORDINATES: protein motif:HMM:NF045039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957486.1"
                     /translation="MSLSVGAFGAACSSTGGGVLGSGGGVSPGAGGASGGSTPAASGG
                     AVASGGAVATGGSGVVSSGGMSTVSGGTSASGGTSAVSSGGASGGVPQSGGAASGGQS
                     TTGGASGGSTGMAGSGTGGTSAGGTSAAGGSNGGTAGSGAGGSSSSGKGFFSDDFEAG
                     TDAMQPPGWENFIGYVKNGANPSSDKSGLVLVDSTRAHSGKKSLHVKGGSTPALATKA
                     LPTGTKKLYVRAWFYMTRKLGQNPGANHETLISLRKDLKGANDEVRFGEIKGVIGTNE
                     VPSDNISPKMDMWGKGPVVAANTWACIEVAFLGDTSPNKLQAWADGTLVHEVTAPDQW
                     QNGMLPANWLDGKFLEVGIGWQSFSSAANELWIDDVVLSDSKIGCN"
     gene            7911..>8774
                     /locus_tag="VFQ61_23470"
     CDS             7911..>8774
                     /locus_tag="VFQ61_23470"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET9957487.1"
                     /translation="MAENLAARRAAWEPSSVRRSLIHQLTFCAAFVTAPALSSAQSVG
                     LQGSVRARSAASEPSVIEKLAVASRVSRGKVSSKPGREAHARPTVNDSRGRDHSVNAV
                     QSALPIQRNQARHSVASGETLSHLAVRYDTSVAKIAALNDLDPEKPLRLGQVLVLPAS
                     ASQPPKSWHPYAKPSKKSGYLEVSTHASRFQGLTVDADGRLRTPAVRALNNLLGAGGP
                     HPPLPERLIRLLIQVSDTFGGRGLRVVSGYRTRSFFQDSRHKVSCAVDFSVIGVPNAV
                     LRDYLREFEDVG"
BASE COUNT         1681 a         2708 c         2767 g         1618 t
ORIGIN      
        1 gatgtgaata gggctgttga tcgggacgga gccgaggcgc agctcgatgc ccgtgccgtg
       61 ttcgagatcg aagccgcgca aggtcacatc gttcccgagc ccctctccgt cttcatgatc
      121 gacgaaaaag cccggcgccg cggctaggag ttcactcgcg tgttgccgtg gcgaagcttc
      181 gagctgctgg cggtcgatgc gtaagtctcc cgcgccgcgc gggctggtcg gtcgctctcc
      241 catgacttgg atctcgcccg cccccgtgag ctgtcgttct tgaccttggg cgcgtgaagc
      301 ccaaaccaaa gcgctcaccg tggtggcgac gagtgggaag ccgaacttgc gcaacagatg
      361 aattctcggc ggacgtaggc caatggcgct gagcgtcaga accctcaact gacgatgaac
      421 tgccccgtca gcggctcggg cccgggcagc gtggacttga attcgtagag cccttcagct
      481 cgaggaaccg caaactcgat ggtcacgctt tcgcccggtt cgaggtgcat gacctgcgct
      541 tcgtcctcga agcacgcttg ctgcgggcaa ccatcgtcat tcggcgtcgg ttgacaggcg
      601 agcaaaaaat tgtgggcgac cgtgcccgag ttagtgagct tgagacgcac gttggcgctg
      661 ttttgggcct tcagaatctt gggggaaaat tcgacgtcgt ttaccgtgac ctcgaattga
      721 taatccccgc cgatgattcc atcgagatcc ccgagctcgc aagcaccctt ggtctcgccg
      781 ctcgaacagg cgcctagcac gagcagagct acgaggcttc ccggggcaaa cctcgcccgt
      841 aaacatcccg ggcgccaacg ggaacgaatg tttctctttt tcttgaaatg cggcatcgag
      901 gaggtgacgg ataacctatc tgtctgaagg gtcgttgcct atccgacagt tgccggaccc
      961 tcgtggcatt ctcgggtcta ctcgggtttt ctagtccgaa ccccgcggta aacgaagtcg
     1021 aagaactgtg cgcggtgggc cacgccgcac ttcgagtaga cctcggtcac gtgttgacgg
     1081 atggttttcg ggctattgcc ttcgcgctcg gcgatttcgg tgctcgaaag cccttgcaat
     1141 aggtaacgtg cgacacgcag ctctttgggc gtcaaccccg catcgtccaa gacgtggggc
     1201 tcaggcccgc gttggacgag tacccgttgc acgacctcga ccaaagcggc cgcactgaag
     1261 ggcttctcga cgaagtaggc cgccccctgg ttgagcgcag tcttcacctt atctacgtct
     1321 gcgaacgcgg tcatgatcac tactggagcg atgaatccgc tggtccgaag gtcgttcatc
     1381 aagtgcagcc cggctcgatc atcgcggccc atcactacat ccgtgatcac cagatccaga
     1441 gcaccgaaac gtcgagcctc gagcagcgct cgtgccggac cctccgcggc cacgcaatcg
     1501 tacccggcgc gtttcagcgc gcgaaggagc aactcgcggg tatcgggctc gtcttccacc
     1561 agtagaatgc gggcagtcat tgcgtcactc cgacgggtag cgtcaaacgg aagcacttcg
     1621 gttctctttc gaagaccaag tcaccatcca tggcccgcgc gagtgcccgt gcaatcgaca
     1681 ggcccaagcc caatcccgtg ctctttgaag caaggcctcc gacgccggct ccccctccga
     1741 ggtgaggtcc tttgctggtt ccgagcggat cgaacagcac ggcttccgta tccgtcgcaa
     1801 ttgctgggcc gtcgtccgtt acggtcatca ccgcacgccc atcgctttcg tagattcgaa
     1861 tcgagatccg gcctgcggca cgggcgtgca acgcgtgaag gctattgtcc acgagatttt
     1921 ccagaatggt tcggagatac atcgagtccg ctcgcgcgaa gcaggcgccc tcggcggtga
     1981 cctccatctg cacgtgttgc tccgctccga ggtgctccag atcgttcacc agcgctcgca
     2041 cggcgctcac caaatccagt cgagcgagct ccggttgacc taccttgcgc agtgagcgga
     2101 gggtccccac ttggcccaag gcgcgctgca cctgcgcgcg agcacgctgc gcgagttcac
     2161 ttcgttcgac cggcgttacg tcctcgagcg cgagttcatc cagcagaaga ccgagcgcgt
     2221 gcaacggatt gttgatctcg tggagtaccg ccgcgatcaa cacccacacg gcttgatccc
     2281 gctccacctt ttggagccta atcttcaaag cctcggctcg ctctgcggcc ttctcggctt
     2341 ctcgcaagcg ccgctgcaac ccgagctggt agagcgcgag cccgaacagg ccgcccgcga
     2401 agacgggcag cagatgatcc acccaattgt gaagagtcgc aaaggcgccg gtcagggtcc
     2461 ccagggccag gcgcttctcc gcaattacat cgaaggcgac gtaagcagag ccgagcagca
     2521 gccccggtac cagataagaa tagagtcggg cgttcgagct ggtcttcacg gggttcgcga
     2581 tcgagtacac tgatgtttcg gaggcggcta cccaattcgg ggcgggcgcg cgtcagggga
     2641 aagtttcgcc gccgcggcgc gatagctcgc caatcgcggt ccagccaatc gtggtccagc
     2701 cattcgtggt ccagccattc gtggcccacc gcgcgtctgc gcgcaggcga caaggcaatg
     2761 agttgggggt gacaacacga atgcgcatcg agggggggaa tgcgatgtag ttggtgcagt
     2821 gcaacacgct ttctcactct gaatccgctc gctcccgctg gactcagtgt tgcttgctgt
     2881 ctctcgcgtt aggcggcaat aggcgtgtgc cgccagctcg cgtcatcgtt tgctcgcgtt
     2941 ccagagctga ataggacccg gggcataggt tgacatagtc actgcttgat ctgtgcttga
     3001 ccatcacccg tctggctgtc actctgtttc gagatcgggg tcgctcgtaa ctcgtaggat
     3061 cgctcgtttt ctcgagcacc aaccgaggag cacgctggat cagaggagga tagtcgtggg
     3121 gatgttcatt caggccgatg acggcacggg tagctttttc ccggccgaag tgggtacaca
     3181 cctgctcacg atcactaact tcgatacggg ctacggtgaa gccccgccca gcggagagct
     3241 cgtggcggtc agctacgcgc gatcgaatag cggtccaccg ccggagcggc tcgacgatct
     3301 cgtcaacaac gcagcgtata cgaacgacac gcgcattcgc ggagtggaca acaacttcaa
     3361 cgtgcccgga gtcgggttga tctccaactt cgccggcgtg gccatcgaag tgggggatgc
     3421 caataacccc tttcccaata cgcaactcac ctattatgac gtcacggaga acgggggtgc
     3481 cggctaccct gcttgtgaca ccagcaacaa tcccatcccg agccccggcc cgatcgtgct
     3541 ctatcacgag ctctcacaca ttcacgacta ccataataac gtcgcgacca ccgagttcga
     3601 cgcgatgacg gacgagaatg agcttcggac tcaaatggga accgagtttg ggcagcgcga
     3661 cccgaacaat cacggaggat gtgggacggc cacgacgacg tgttgcgttg tcgccagtat
     3721 cgctacgggt tggccccggt ccccgcagct gcacgagctc aggaaggtgc gcgaccgctt
     3781 gttgcggcgt agcgcggttg gcttcgagtt tttcgaggca ctgttccgcg actactacta
     3841 cttcagtccc gaggcttgcc gcctgctcgg gcaaaactcg cagctctcgc gtctggtgcg
     3901 ggatcggctg gtcgatccac tcgtgatcgc gttacgccta gtggacagtt ggcggccgac
     3961 accgcccgct ccgagtggcg cagccctgca attcgaacgc gagcgaagcg cgcatccggc
     4021 actagcggcg ctgtccgtgg ccgagctcga gtggagcgtg gcggcattgc ggagcgtggc
     4081 aacgggagcc acggattcgc gcttggaaga gctcggtatg ccggcggagg tcgtggctct
     4141 ggctcggcgc gtaccccgga gcgcggccgt gatgtgggca ttcgtggagc cgatcactct
     4201 gacttattcg gctctgctag aacacgccaa gtccgctcta ctagattcgg ccgggctcga
     4261 tcgccgcgat ggctcccagc aggaacaact cgagtcgggg caccgcgagc gggggcagcg
     4321 cctactcgca gcgttcgagg gctggagtgc ggggctaccg ctcacgccag ggtggcagcg
     4381 ttggacttgc ctcgaaatcg agcagcagct tcggttcttg agtgaggttt tgctgctcac
     4441 gagtcggtcg cgagctcgct tccggacccg gatgttggga gagctcgaag ccggtcccga
     4501 ggtgcccgcc gggagcgccc gtcccgctgc ggatgacccg cgtttgctct cgagtttgga
     4561 gcgttggggt tacttcggag gagaatatga cgcgccttgc cattgaagaa tttcggatgc
     4621 gggacaacgg agaacgtctg gagttctcgc tgcggatccg aaacgacgag gaccgaacac
     4681 tgcacatgat cgcggcccct cgcggttatg agtgggatgc cgcgacacgg cgcttcaccg
     4741 taaaactgca cgatactccc ctcgatccag cttcgcgcgt gcaagtggcc ttgttcgtcc
     4801 ccccaacgtt caaagagcta gagccggggc gagtcaccga ggtctccttc acggtgccgc
     4861 gcgtgctcaa gacagccgac gcgagcggag ccgtcgtagt ttggcccgtt tggaacgcac
     4921 tcgaggtacg agttgctttt gcagtctcgg atactcccct ttaccccgtg gtggacgaag
     4981 cgcgtcgggc ttccgggctc gcggcccctt tgcagctcgc ggccgcgtcc gccgcgtggg
     5041 gagagagagt cgaaatctcc gctcccgccc gcgcgtcgct cgaaactcac tgaaagcgca
     5101 ttcgttgcgt agtgaacgag gagaagtcaa tggccacact tgccgaaatt gccaatcaga
     5161 tcaacaccac tctgtctcaa atttccagca atacccagga caccgcgacc accgccggtc
     5221 taatcaaggg cgacaccgcg gatatcaaga cacgcctcga taacatccga gccactctac
     5281 aggccggctt caccggactc ggactagggt tgtttgcctg cctcgagcaa ctcaagaagg
     5341 gcaatagcct gctcgagcta aacgcggccc aaaacgaaac catcatttgc tggctcggca
     5401 agctcgccga cctcgaatgc cggcagctgc gcgtcgccga gcacatgctg gagacgacgc
     5461 gagccatcgc gctggacgca cgaaaggtcg cgcagatcac ggaacacgtg catgccgacg
     5521 cagccctgac cgtggctcac gagaatcggt tggaggagcg gattcgtcag tgctgcgacg
     5581 ctccgccgaa ggaccccggt ccttgtttcg agccgtgccg tcaacgcgaa gtagatatat
     5641 acgaaccgcg cggcgattgg cagccgccgc ggccaaacgg gggagaagta ggatgacatc
     5701 gcgacgcggc gccgaacatc atcgcaacgg cgcgcggggc tggaccgtgc ttgccgctac
     5761 cgcgcttgcc gctacctggc tagcagcgag cttatctacc ggttgcaagt gcgacccgga
     5821 gaaggtggaa gttccaccgg acgattgcgc gtcacgcccg gccgcggcaa gcgttacgat
     5881 ccgagccagc ttgtctggga ttcgccctat cgggagcgac aaacagctga agatcaacgg
     5941 caatcgagag ccggggcaga gctcctgctt tacgggagac accaacgtgc tcacgaccct
     6001 gaccggccaa tccgaggtca gcgaagcgca cgcgctcgcc catggtgcct ggaagttcac
     6061 ggtcaccccg ctttcgggcg gggatcatgc cgccgtcccc gagcagttgc gcacgctggc
     6121 gcccggctcg agccagacat tgcgcatcac atcgacctcg tccggcgata tcgcgattgg
     6181 ctgggaatag cgggctcgaa aagccatggc cctgttcatc gatacgactg atctagcgct
     6241 atccattggc gcaaacaccc gccgagtctg gtcgagtttg gcagtcgggt aagtgcagag
     6301 cccgcgggat tgggggcgcg acaatcggga gacaaccact cgagcgcagc gaccggaatc
     6361 cacggttcct gaaataagcc gcttgcgccg ttcttcacga ttcaattggc tcagagacag
     6421 tcagaatctg cgcagtctcg gtgaggcggg gcagttggcc cgaggctcga aggaccggga
     6481 aatgacggaa gccacgtgca ctcgcgtgtg atacgccaag ggccttgcgg agaaatgaaa
     6541 agtgagtcga cgaatcggcg tagctagttt ccttgtggtg agtctctcag tgggtgcgtt
     6601 cggagccgcc tgttcctcga cgggaggcgg cgtgctgggc agcggcggcg gcgtttcccc
     6661 gggcgcgggt ggagcttcgg gcggcagcac tcccgcagct tccggcggtg cggtcgcatc
     6721 cggcggtgcc gttgcgactg gtggcagtgg tgttgtgagc tcgggcggca tgagtaccgt
     6781 ttcgggcggc accagcgcga gcggtggcac tagcgccgtg agcagcggcg gtgcttccgg
     6841 cggagttccc cagagcggtg gtgctgcgag cggcggtcag tcgacgaccg gtggagcttc
     6901 ggggggcagt acgggaatgg caggaagtgg cacgggcggc acgagcgccg gcgggaccag
     6961 cgcggcgggt ggcagcaacg gcggcaccgc gggcagcggc gcgggcggca gctcgtcgtc
     7021 gggcaaaggc ttcttctcgg atgatttcga ggccggcacc gacgccatgc aaccccccgg
     7081 ttgggaaaac ttcattggct acgtgaagaa cggcgccaac ccgagcagtg ataagagtgg
     7141 gttggttctc gtcgattcga cgcgagcgca cagcggaaag aagtccttgc acgtcaaggg
     7201 cggcagcact ccggccttgg caaccaaagc cctaccgacc gggaccaaaa aactttacgt
     7261 gcgagcctgg ttctatatga ctcgcaagtt gggccagaat cccggcgcca accatgagac
     7321 cctgattagt ctgaggaaag acttgaaggg cgccaacgac gaggttcgct tcggcgaaat
     7381 caaaggcgtg attggcacca atgaagttcc gagcgacaac atctctccga agatggatat
     7441 gtggggcaag ggtccagtgg tggccgccaa cacgtgggct tgcatcgaag tggccttcct
     7501 gggcgacacc agcccaaaca agctacaagc ttgggccgac ggcacgctag tgcacgaagt
     7561 caccgcgccc gatcagtggc aaaacggcat gctgccggcg aattggctcg acgggaagtt
     7621 cctggaagtc ggcatcggct ggcaaagctt cagcagcgcg gccaacgagc tctggatcga
     7681 cgacgtcgtg ctgagcgact cgaagattgg ctgtaactga acggcagttc gaagcagccc
     7741 tgcactcgag atgagccgcg gcgcacgaac tccgcggctc atctgttttg tgtgtgtcgc
     7801 cttcagccag cgccgagagt gaatcgagcg aggagtttgg cgaccagaga attgcgtgac
     7861 cagagaattg cgcagccagc ctagattctg atggtctgcg agcggcggcc atggccgaaa
     7921 acttggccgc acggcgcgcg gcatgggagc ccagttccgt gcgtcgctcc ctcatccatc
     7981 agctgacctt ctgtgcagcg ttcgtcacgg cgcccgcgct atcctccgcg caaagcgtgg
     8041 gactgcaagg ctccgtcaga gcccggagtg ctgcgagcga acccagcgtt atagagaagc
     8101 tcgccgtggc gagtcgcgtc agcaggggca aagtgagctc caagcccggg cgagaggccc
     8161 acgcacgccc gaccgtaaac gattcacgtg gtcgcgatca ctccgtcaat gcagttcaat
     8221 ccgctctgcc cattcagcgc aaccaagcgc ggcacagcgt ggcctctggc gagaccctca
     8281 gccaccttgc ggtgcgctac gacacgagtg tcgccaagat tgcggccctg aacgatctgg
     8341 atccagagaa gccactccgg ttgggccaag tcctcgtgct tcccgcctcc gcttcgcagc
     8401 caccgaaatc gtggcacccc tacgccaagc caagcaagaa gagcgggtat ctggaggtct
     8461 ctactcacgc ctcgcgtttc caaggcctga ccgtagacgc agacggtcgc ctgcgaacgc
     8521 ctgccgtgcg cgcgctcaat aaccttctcg gcgcaggtgg gccgcatccc ccgttgccgg
     8581 agcgcctaat tcgcttgctc atccaagtga gtgacacgtt cggagggcgt gggctccgtg
     8641 tcgtcagcgg ctatagaact cgctccttct tccaggactc gcgtcacaag gtgagttgcg
     8701 ccgtggactt cagcgtcatt ggcgtgccca acgcggtatt gcgggattac ctccgtgagt
     8761 tcgaggacgt cggg
//
LOCUS       DASTKU010000675         9527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1692182, whole genome shotgun sequence.
ACCESSION   DASTKU010000675 DASTKU010000000
VERSION     DASTKU010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9527
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692182"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..1043)
                     /locus_tag="VFQ61_23475"
     CDS             complement(327..1043)
                     /locus_tag="VFQ61_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9957488.1"
                     /translation="MTLKTLKAGFKRPFEWKAILIQLEVLGVASVGIVTVTSVFIGMV
                     MAVQFAFGLRKFGGMEYTGRVIALSFARELAPTLTAVIVGGRIGSGMAAEVGAMAVTE
                     QIDAVRALGADPLKKLVWPRLVASVIVMPMLGAFALVLGFAGAMMIADLQFDIPAEFF
                     LRTALSTVTMADFISGMLKTPVFGAIIALVGCHFGMTTTGGTAGVGNSTTRTVVVISI
                     AILIADFFLTKVAIILWPTA"
     gene            complement(1196..1735)
                     /locus_tag="VFQ61_23480"
     CDS             complement(1196..1735)
                     /locus_tag="VFQ61_23480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957489.1"
                     /translation="MRLVRCSSALGACLPSLKLASLVFALCVLSACARSSFDGQVYRD
                     SEVAFRVGQLPPSFHAVKAEGTRLAFRDDANDITVAVNGRCGLDGDDVPLPALTRHLF
                     IHFTQREVISERRVELDGREALRTELSAELDGVPRRFVVYVLKKDGCVYDFMWIARPE
                     ATASVTEFERFVLGFSTRI"
     gene            1875..2600
                     /locus_tag="VFQ61_23485"
     CDS             1875..2600
                     /locus_tag="VFQ61_23485"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9957490.1"
                     /translation="MLPIGDQNPTRTRPYVNYLLILANVIAFVWQYLLTSTGGEAWIV
                     AGYGLVPVRFANDPLGEAFTLFTSMFMHGGIGHLVFNLLYLHIFGDNVEDAMGHIKYL
                     VFYLACGVAAGLVQVGVDPGSTVPMVGASGAIAGALGGYIVLYPRAPITVLNPIPFLW
                     LFFGLFLVLPAWLVIGLWFGGNLMSGLAELGEMESGGVAFFAHIGGFVAGMLTVRGFV
                     LGRQRADRYSNFTPAPPRVYYRR"
     gene            complement(2758..3555)
                     /locus_tag="VFQ61_23490"
     CDS             complement(2758..3555)
                     /locus_tag="VFQ61_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9957491.1"
                     /translation="MLWSLDPLVYAKRVILARYGMRLGILSDIHANYEALSAVIEAYR
                     HENIDQYYCLGDTVGYGGSPNECSDLVRNLVVATILGNHDAAVAGRMDYSYYYEAARH
                     ALDLHAQLLSAENMAWLKGLPYQIKLEDLGVLLCHGSPVRLEEFEYIFAPEQARECLP
                     IYDQLGDLTLIGHSHLCKVFALTPTSVEELPPIDFELLPNRKYIVSVGSVGQPRDYDN
                     RASYTVFDTTKRRFEFKRVEYDIETAADKVLRAKLERNFAHRLFIGV"
     gene            3960..4535
                     /locus_tag="VFQ61_23495"
     CDS             3960..4535
                     /locus_tag="VFQ61_23495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957492.1"
                     /translation="MIPTPPPVEDLVCVALDYVERATGIRLDFHEETLPVLDHYAATV
                     RAEGERNPGLISLIAPALGAYFGELVRSTFGGFWRVPTPNQHDWAVCLETTFLAFNPI
                     GVAFDSVAGGNDHEGPRSALRIAPEDREYLEQRLEAIPPVPEEEYFLFSTRFEVLQVA
                     VEALRAKMEEEGYGGTEYSADDYTLDYGSGA"
     gene            complement(4567..5871)
                     /locus_tag="VFQ61_23500"
     CDS             complement(4567..5871)
                     /locus_tag="VFQ61_23500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957493.1"
                     /translation="MNGVARARRNCPAAPSWVACAAIALVVGCAESKTKPPERSTEPS
                     PNANIVPAPLSNEESNAQRSHVSDAGVPSADPSGEVGPAPEVRGLWERSALSADMAFK
                     EPVGVRLTARLRWLDLPSFPRAPETNVEVLARLREASTFDLEISLLPAGRMRIVFDSD
                     AFSLPRGSELRARSDRLGHLLVWDAASRYTILPAGALRAVFTEHRPDVLPLVKPKVMP
                     GTPGNWLSMPTTKVELATTFGRLVVEQANIPNAGAAGELLCRTLSELVAADPLNPACI
                     KGQLALRAELFSRSNGRMSFEVLRIDRERALEPSSLLVPPADARALIAELPNPARAVF
                     PSEERLRELRLRPLPRAEKLDPSAPKQGLWIQNRSDVFRYVLLDGAMLARVAPRSDLR
                     VDALTPGRYSWLALDFLGDEPTPLRSVEIPARIVIGEESEAK"
     gene            complement(5868..6320)
                     /locus_tag="VFQ61_23505"
     CDS             complement(5868..6320)
                     /locus_tag="VFQ61_23505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9957494.1"
                     /translation="MDERAAPALIAPAEPRDEATLTRIMQECGLSFEFRKELNRPYAR
                     IWVARPHAEGEAQGVLVAWQVADEAHLIDLAVDPAFRRRGVARALLRHLISSSLRQGL
                     RLIALEVRSGNDPAIRLYRAFEFEEVGERRGYYADGEDARLFSLRLLP"
     gene            complement(6450..7838)
                     /locus_tag="VFQ61_23510"
     CDS             complement(6450..7838)
                     /locus_tag="VFQ61_23510"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9957495.1"
                     /translation="MTARILVVDDSPTIRKVVSMILAARSYEPFLAGDGKEALDLLEA
                     QPVDLVLLDFVMPRMNGYQFCRELRARRHLHALPVILMSAKSDKIRGQFVQQTGAVDA
                     ITKPFDARGLVAAIEGALKRKREGRLRPVPDASSMPLEESGLEPSGRNSDVGPSSLEF
                     VDNPRGGGLIASLEELLAPELELLAPELERRTLRKALERAFAPDVLGRVYSLSRALSV
                     GPESREALAGDISVISIAEVLQMLELQRQTGSLSVHTRKQEITLHIRDGKLDWAGSHG
                     LGDEFLIGRYLVESGVLSRDRLEKIMLLHPGPKRLLGETLVEAGLVAQEQIDKALRRQ
                     SSELVYEAVRWKKGRFAFTARQENLIAEKASLGLETAALVMEGFRRVDEWRVIEGSFD
                     FDEVLYPDPVAIERLGNDAMLTRRERRVLEAIDGERTIREIVSEMGGSSFELCKIVYQ
                     FLNSRLVRRRAA"
     gene            complement(8351..9196)
                     /locus_tag="VFQ61_23515"
     CDS             complement(8351..9196)
                     /locus_tag="VFQ61_23515"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET9957496.1"
                     /translation="MSQPTRLLIIGFGLIGGSVALGVRKAEPRSFHVTAVDVPEVLAL
                     PEARAAADSLIEISDWTRVERALSECTLALLAAPVRVICDQVLRVLSLANVVTDCGST
                     KRHIVERAAKSPRAGRFVPGHPMAGLPEGGLSRASGELFVARPWIVCPERVDTDALMH
                     VESLVETLGARLMRMSAEEHDAAVARTSHLPQLIGSALTLIGSPYASSAAGPAFERAT
                     RVAGGPESMWRDIFATNGDEIARAIQELCGELEPIGRELGADVGRTALADALLARARK
                     QRSQS"
     gene            complement(9193..>9527)
                     /locus_tag="VFQ61_23520"
     CDS             complement(9193..>9527)
                     /locus_tag="VFQ61_23520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957497.1"
                     /translation="ELARGGTSVVLLEAGGEYPNGARTLEIAAIARAKQRTHLPIVVD
                     VPSIAQKGRYCAAVACAAIAAGADGVILRAWVGPDGEIPRVPATLQWADAVALAEKLR
                     AIGGVVRR"
BASE COUNT         1706 a         3066 c         2993 g         1762 t
ORIGIN      
        1 gatctcggga gtgtgcgagc agtcaggagc catcactcga atctgctact atgggaattc
       61 tctcgaaaca tcgagtggaa gctaggctga acgcgcactc tcttcttggt gtgcaatcgt
      121 tttccgcggc ctgctctcga aacgtacccg tgagtcgcgt gtggttcgcg caacgacgag
      181 ttccgccgtt caattgcgag gccgcgtcat cgcgagtcgg ggaatcatga tcgaagggat
      241 agcgagtttg cgccgccgtg ctagggccgc ggcgacccaa acacccttgc ttccaactcg
      301 gtcgttcgtc ggctacgccg ggtgcatcaa gccgtgggcc atagaatgat cgctaccttc
      361 gtgaggaaga agtcggcaat caggatggcg atggagatca cgaccacagt gcgcgtcgtg
      421 ctgttgccca cgccggccgt accccccgtg gtcgtcattc caaagtgaca acccacgagc
      481 gcaataatgg cgccgaacac gggggtcttc agcatgccgc tgatgaaatc ggccatggtc
      541 accgtgctca gagcggtacg caggaagaat tctgcgggaa tgtcgaactg cagatcggcg
      601 atcatcatgg cgcccgcgaa tccgagcacg agcgcgaacg ctccgagcat cggcatgacg
      661 atcaccgaag ccacgagccg tggccacacc agctttttca agggatcggc gcccagcgcg
      721 cgcacggcgt cgatctgctc ggttacggcc atggcgccca cctcggccgc cattccggag
      781 ccgatgcggc ctccaacgat aacggcggtg agcgtgggtg cgagctcgcg cgcgaaggac
      841 agcgcgatca cgcggcccgt gtattccata ccgccgaact tgcgcagtcc gaaggcgaac
      901 tgcaccgcca tcaccatgcc gatgaacacg ctcgttacgg tgacgatgcc cactgaggcc
      961 acgccgagca cctcgagttg aatcaggatc gccttccact cgaagggacg cttgaacccc
     1021 gccttcaggg tcttgagcgt catctcggag acggccccga gatggccgaa gaaacccagt
     1081 gcatgctccg accaccgcga caacagccgt gcgggtgggg gcggcgcgag gctcaagcgt
     1141 gagcgacggc tctctggttc gtcggtcgtg tcagacacgg gcaagcacgg ggttatcaga
     1201 tccgagtaga aaatccgaga acgaagcgtt cgaactccgt cacactcgcg gtcgcttcgg
     1261 gtcgagcgat ccacatgaaa tcgtatacgc agccgtcctt cttcagcacg tacaccacga
     1321 accgacgcgg tacgccgtcg agctccgcgc tgagctcggt ccggagtgct tcgcgtccgt
     1381 cgagctcgac gcgacgttcc gagatgactt ctctttgcgt gaagtggatg aacaggtgtc
     1441 gcgtcaaagc cggcagcggc acgtcgtcac cgtccaatcc gcagcgccca ttgacggcga
     1501 ccgtgatgtc gttggcatca tcacgaaacg cgagccgcgt gccttcggcc ttcacggcgt
     1561 ggaaactcgg agggagttgc ccgacgcgga aggcaacctc ggaatctcga tatacctgac
     1621 cgtcgaacga agaccgcgcg cacgccgaca gcacgcacag agcgaacacg agagaggcca
     1681 gcttgagtga aggcaggcag gccccgaggg cagaactgca ccggacgagc cgcatgattg
     1741 cgccggacgt tagcacggcg gaccgcgtga ggtccccttt cccgagctcg aaaatcggcc
     1801 gttcgcagcc ggtccgctcg aaatgggcgg ctcccccaac gcgaccgtga attgtagcta
     1861 gacttcgaag cgcaatgctg ccgatcggcg atcagaaccc cacgcgcacg cgtccgtacg
     1921 tcaattacct gctgatcctg gccaatgtga tcgccttcgt gtggcaatac ctactcacgt
     1981 cgacaggcgg cgaagcctgg atcgtcgcgg gttacggcct ggttcccgtt cgctttgcga
     2041 acgatccgct cggtgaggcc ttcacgctgt tcacgagcat gttcatgcac ggtgggatcg
     2101 gccacctcgt attcaacctg ctctacctgc acatcttcgg ggataacgtc gaagacgcga
     2161 tggggcatat caagtacctg gtcttctact tggcgtgtgg tgttgccgca gggctcgtgc
     2221 aggtcggggt ggatcccggg agcacggtgc ccatggtcgg tgcctccggc gcgattgcgg
     2281 gagctttggg tggctatatc gtgctctacc cgcgtgctcc gatcacggtg ctcaacccga
     2341 tcccctttct ctggctgttt tttggcctat tcttggtact tccagcctgg ctcgtgatcg
     2401 ggctctggtt cggcggaaac ctgatgagtg ggctcgcgga gctcggcgaa atggaatctg
     2461 gtggcgtcgc cttctttgct cacatcggag gcttcgtcgc gggcatgctc accgtacgtg
     2521 gcttcgtgct gggtaggcag cgggccgatc gctacagcaa cttcacccct gctcccccgc
     2581 gggtgtatta ccgacgctga tccgcaacgg taactcgaga attcatcgcc ccgtcgctcg
     2641 gcagttggta acggggccag cgacggcctc gaaatcagcg gacaagacgg cagctctcgc
     2701 cgcgcaggcg tgcgcgacgg cgctcagacg ccgatggcgc tcagacgccg atggcgctca
     2761 gacaccgatg aataaccggt gtgcgaagtt ccgctcgagc ttcgcgcgca gcaccttgtc
     2821 cgccgcggtc tcgatgtcgt actcgacgcg cttgaactcg aagcgacgct tggtcgtgtc
     2881 gaacacggtg tagctcgctc gattgtcgta gtcgcggggt tgcccgaccg agccaacgct
     2941 gacgatgtac ttgcggttgg ggagcagctc gaaatcgatc ggcgggagct cctctacgga
     3001 ggttggcgtg agcgcaaata ccttgcagag atgcgagtgc ccgatcagcg tcagatcccc
     3061 gagctggtcg taaatcggca aacactctcg cgcttgctcg ggggcgaaga tgtactcgaa
     3121 ttcttccaag cgcacgggcg agccgtggca cagcagcact ccgagatctt ccagtttgat
     3181 ctggtaaggc agccccttca gccaggccat gttctcggcg gacaagagct gcgcgtgcag
     3241 atcgagcgcg tgccgggcgg cctcgtaata gtacgagtag tccatgcgac cggcgacggc
     3301 cgcatcatgg ttgccgagga tggttgccac caccaagttc cgaactaggt cggagcactc
     3361 gttgggcgag ccgccgtagc caaccgtgtc tccaaggcaa tagtactggt cgatgttctc
     3421 gtgccggtac gcctcgatca cggcgctgag cgcctcgtaa ttggcgtgaa tgtcgctgag
     3481 gattccgagg cgcatgccgt aacgcgcgag gataacacgc ttcgcgtaaa ctagagggtc
     3541 gagcgaccac aacatttccc gaccccggtc gctaacgcaa gccctacgga acaggtttcg
     3601 cagtttaggt ggctttttct tttcttcacc cgtccgttca ccgggctgcg gagctgtgtt
     3661 gcctcggagt tgcctcggga aaggtgcctc cgacgaaggt gcctcggcga aaggtggttt
     3721 ctgggcaggc gtggtcgggg gttcttaaag cgggtcggac gaggcggccg gagtttctct
     3781 cggtgcggcc aaaaaggcgg agttgttgcg gaacggtcgc gtcacgggtg cccgcgtcag
     3841 ctttgccgac gtggaagcgg tcgggtgccg ggagacagtt gagctcgctg ctccccacgt
     3901 gcaggctgaa atgaaatgct catgtgggcg aacggcccca gagtcgctat ggtggtcgcg
     3961 tgattccgac gcctcccccc gttgaagacc tggtctgcgt tgcgctggac tacgtcgagc
     4021 gcgctaccgg cattcgcctc gactttcacg aagaaacttt gcccgtcctc gatcactacg
     4081 cagccactgt tcgcgcggag ggcgagcgca atccgggctt gatttcgctg attgcgccgg
     4141 cattgggcgc ctacttcggt gagctcgttc gcagcacttt cggcgggttc tggcgagttc
     4201 cgacaccgaa ccaacatgac tgggccgtgt gtttggagac tacatttttg gcctttaatc
     4261 ccataggggt ggcgttcgat tcggtcgccg gtggcaacga ccacgaaggc ccccgctcgg
     4321 cgctacgcat tgcccccgag gatcgcgagt acctggagca gcgcttggag gcgatcccgc
     4381 cggtacccga agaagagtat ttcctgtttt cgacccgatt cgaagtactg caggtggccg
     4441 tcgaggccct ccgcgcgaag atggaggagg agggttacgg cggaacggaa tattccgctg
     4501 acgactacac gctcgactat ggctcagggg cgtgatcggt ttgagtgtga ccatgaagca
     4561 ggcgcatcac ttcgcctcgg actcttctcc gatcacgatg cgcgcgggga tctcgacgct
     4621 gcggagcggc gtcggctcgt ctcccaggaa gtccagcgct agccacgaat accgtcccgg
     4681 cgtgagggca tccacgcgta ggtcgctgcg cggggcaacg cgagccaaca tggcgccgtc
     4741 gagcagcacg taacgaaata catccgaccg attctggatc cagagtccct gtttgggagc
     4801 agaggggtcc aacttttccg ccctgggaag cggccgcaag cgcagctcgc gcaggcgctc
     4861 ttcactcggg aagaccgctc gggccgggtt cggcaactcg gcgatcaacg cccgagcatc
     4921 tgcggggggg accagtagcg aactcggctc caacgcgcgt tctcgatcga tgcgcaggac
     4981 ctcgaacgac atgcgaccgt tgctcctcga gaacaactcg gcgcgtaacg cgagctgacc
     5041 cttgatgcag gccgggttca acggatcggc ggccacgagc tcgctcaacg tgcggcacaa
     5101 gagctcgccc gccgcgcccg cattcgggat gttcgcctgt tcgacgacga gccgtccaaa
     5161 tgtcgtcgcg agttcgactt tggtggtcgg catcgatagc caattgccgg gcgtgcccgg
     5221 catcaccttg ggtttcacca gtggcaacac gtccggccga tgctcggtga acaccgcgcg
     5281 cagcgcacca gcgggcaaga tcgtgtagcg gctggccgcg tcccaaacca agaggtgacc
     5341 gaggcggtca ctgcgagcgc gcagctcgct cccgcgcggc aagctgaagg catccgagtc
     5401 gaagacgatg cgcattcgtc cggcgggcaa taggctgatt tcgagatcga acgtcgaggc
     5461 ctcacgtaag cgagcgagca cttcgacgtt cgtctccggt gcccgcggaa aggatggcaa
     5521 atcgagccag cgtaggcgag cggtgaggcg caccccgacc ggctctttga aagccatgtc
     5581 agcgctcagc gcgctgcgct cccagagccc ccgcacctcg ggcgcgggcc ccacctcccc
     5641 ggacggatcc gcgctcggta cgccggcgtc ggagacgtgc gagcgttgcg cgttggattc
     5701 ttcgttactc aacggcgcgg gaacgatatt cgcgttgggg cttggctcgg tgctgcgctc
     5761 cggcggcttg gtcttggact ccgcgcagcc aaccactagc gcaattgcag cgcaggcgac
     5821 ccagctcgga gccgcggggc agttgcgcct cgcgcgcgcg accccgttca tggcagaagc
     5881 ctgaggctga acaggcgagc gtcctcgcca tccgcgtaat agccgcggcg ctccccgact
     5941 tcctcgaact cgaacgcacg atacagacgg atcgcgggat cgttgccgct gcgcacctcg
     6001 agcgcgatca accgtagacc ttgccgaagc gagctggata tcaggtgccg cagcagggcc
     6061 cgcgcaaccc cgcgccggcg aaacgccggg tcgaccgcca aatcgatcag atgcgcttca
     6121 tctgccactt gccacgcgac gagcactccc tgagcttcgc cctcggcgtg aggtcttgcc
     6181 acccatatcc gagcgtatgg tcggttcagc tccttacgga actcgaagct taggccgcac
     6241 tcttgcatga tgcgcgtcaa cgttgcttca tcgcgtggtt cggccggtgc gatcagagct
     6301 ggcgcggctc gttcgtccat tcgaccgcgc agtttcccag tccacgtgcg cgctcgcaag
     6361 cggctcgttc gacaccccgt gcggcacggt ttcgatacga ctgttctgcg tggaccgggc
     6421 gggcgagcga gccgggagac ggcctcaggt cacgctgctc ggcggcgcac caaacgcgaa
     6481 ttcaaaaact gatagacgat cttacaaagc tcgaacgagc tcccgcccat ctcactcacg
     6541 atctcgcgga tcgtgcgttc gccgtcgatc gcttcgagca ctctccgctc gcgccgcgtg
     6601 agcatcgcgt cattgccaag gcgctcgatg gcgaccggat ccgggtacag cacctcgtcg
     6661 aaatcgaagc tgccttcgat cacgcgccac tcgtcgactc gtcggaagcc ctccatgacc
     6721 agagccgctg tctcgaggcc gagtgaagcc ttctccgcga tcaagttttc ctgtcgagcc
     6781 gtgaaggcaa agcgaccctt cttccagcgt acggcctcgt aaaccagctc cgaagattgc
     6841 cgacgcagtg ctttgtcgat ctgctcttgg gcaacgaggc cggcctccac caacgtctcc
     6901 ccgagcaggc gctttggccc ggggtgcagc agcatgatct tctccagccg atcccgcgac
     6961 aacacgccgc tctcgactag atagcgaccg atcagaaact cgtcacccaa gccgtgagac
     7021 ccggcccagt ctagcttgcc gtcacggatg tgcagcgtga tctcttgctt gcgcgtgtgg
     7081 acgctcagcg aaccagtttg gcgctggagt tccagcatct gcagtacctc cgcgatggag
     7141 atcaccgaga tgtcgccggc gagcgcctcg cgcgactccg ggccaacgga gagtgcacgc
     7201 gacaaggagt agactcgccc cagaacgtcg ggagcgaagg cccgctccaa agctttgcgc
     7261 aaggtgcggc gctccaactc cggtgcgagc agctcgagct cgggcgccaa caactcttcg
     7321 aggcttgcga tcaagccgcc cccgcgtggg ttgtctacga actccaagct ggacgggcct
     7381 acgtccgagt tacgtccgct gggctctaga ccggattcct cgagcggcat cgagctcgcg
     7441 tcgggcacgg ggcgcagccg cccttcgcgt tttcgcttga gagccccttc gatggctgcg
     7501 accaagccgc gggcgtcgaa gggtttggtg atcgcgtcca ccgcgccggt ctgctgcacg
     7561 aattgaccgc ggatcttgtc gctcttcgcg ctcatcaaga tcacgggcag ggcgtgtagg
     7621 tggcgacgcg cgcgcagctc gcggcagaac tggtagccgt tcatgcgcgg catcacgaag
     7681 tcgagcagaa ccaaatccac cggttgggct tcgagcaagt ccaacgcttc tttgccgtct
     7741 ccggcgagga acggttcgta agagcgcgcg gccaagatca tggacacgac cttgcgaatc
     7801 gtcgggctat cgtcgacgac cagaatcctc gccgtcatgg tttgcaccgc gacgctggca
     7861 ccagtgttcg tctttcgttc ggcacgttac gcggctctgt tccttgtttc ttttgcgcgg
     7921 ccgtgtcttg cgcggcccgg tttgggcccg ggttcggcca agcgtcttgg gcgttgttca
     7981 agccatgtcg tttcgttacg ccgcaggctc tttccttgca ccgtaggctc tttcgttgcg
     8041 ccgtgggccc ttcaacctag gggcttacct gcttcgattc tcgtcggggc ttcgtagctt
     8101 gctgttgcgg gtgggcggca gctcgagctg ggccctttcg tggttttggc gttagcacat
     8161 atggtgcagc cgctcggcgg gtttcgtcca gcggcttcgg catgggggca aaaccctatg
     8221 gccgaacgca ttctacgcac acgaatccgg tgtcgccgac tgcgcactcc gggtgtaccc
     8281 ccacttacaa agtctcggct tgcgcacacg cggagtaaag ctgagatcgg gcgccgagcg
     8341 atcgagtcgt tcagctttga gaacgttgct ttcgggcgcg cgcgagcagc gcatctgcga
     8401 gcgcggtacg acccacgtcc gccccgagtt cgcgcccgat gggttcgagc tcgccgcaaa
     8461 gttcctgaat cgcgcgagcg atctcgtccc cgttggtggc gaatatgtcg cgccacatcg
     8521 attcgggccc acccgcgacg cgtgtggcgc gctcgaatgc aggcccggcg gcgctgctcg
     8581 cgtagggcga gccaatcaga gtcaatgcgc tgccgatgag ctgcggcaaa tggctcgtcc
     8641 tcgcgaccgc ggcatcatgc tcctccgcgc tcatccgcat gaggcgcgcg cccaaggtct
     8701 ccacgagaga ctcgacgtgc atcaaagcgt ccgtgtccac gcgctccgga cagacgatcc
     8761 aggggcgtgc aacgaacagc tcccccgagg cccggctcaa gccgccctcg ggtagcccgg
     8821 ccatgggatg gcccgggacg aagcgtcccg cacgcgggct cttggctgcg cgctcgacga
     8881 tgtgtcgctt ggtggagccg caatccgtga ccacgttggc gagcgagagc acgcgcagca
     8941 cttgatcgca gatcacccgc accggcgccg ccaataacgc cagcgtacat tcgctgagcg
     9001 cgcgttcgac gcgcgtccaa tccgaaatct cgatcaacga atccgcggct gctcgcgctt
     9061 ctggcaacgc caacacctcg ggaacatcca ccgcagtcac gtgaaacgat cgcggctcgg
     9121 ccttacggac acccaaagcc accgagccgc cgatcagccc aaacccgatg atcaagagtc
     9181 gggtaggctg actcatcggc gcaccacgcc tccgatcgcg cgcaatttct cggcgagcgc
     9241 gaccgcatcc gcccactgca acgtggccgg cacgcgtggg atctcgccgt ccgggccgac
     9301 ccaggcacgc aagatcacgc cgtcggcgcc cgccgcgatc gccgcgcaag ctaccgcggc
     9361 gcagtagcgg cccttctgcg cgatactcgg cacgtccacg acgatgggca gatgcgtccg
     9421 ctgcttggcg cgggcgatcg cggcaatctc gagcgtgcgc gcgccattgg gatattcacc
     9481 acctgcttct aacagcacga cgctcgtgcc cccgcgcgcg agctcac
//
LOCUS       DASTKU010000676         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_798345, whole genome shotgun sequence.
ACCESSION   DASTKU010000676 DASTKU010000000
VERSION     DASTKU010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798345"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..746
                     /locus_tag="VFQ61_23525"
     CDS             162..746
                     /locus_tag="VFQ61_23525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957498.1"
                     /translation="MKSEQRLALDMRGRSKRGCNPDRTLQPDAHEVHVLVYEHGTLGS
                     GERLPSPDRSLELTVLAQSQGESPAEFTQRVLRQVRALNASGRRIRKAGIVLNSGHST
                     EAMQAREMIGRSVLNRFPEDHSGSGAQGGSGPESWAEFVAPGAGETSPEYRYQLFNLV
                     DQLMASAPDSQTAVRIRFRPSELSQELARVPRVA"
     gene            complement(756..1091)
                     /locus_tag="VFQ61_23530"
     CDS             complement(756..1091)
                     /locus_tag="VFQ61_23530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957499.1"
                     /translation="MKPRYYRSDSLITDMCDADAHWGPLLFLRPAQDERFDWRRQALF
                     ALIVGTALGGLGSVILGVLARVFGRAPDIAYSFPLVLTLVYFAACVGVVAPAWNRRAM
                     RLARLRTYR"
     gene            1306..1776
                     /locus_tag="VFQ61_23535"
     CDS             1306..1776
                     /locus_tag="VFQ61_23535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957500.1"
                     /translation="MRTPPSSEGNTCEFRVGRLMEIRVPNGYRTADDVDRMVARMTSL
                     SLSLARSEKFIIAADWRKVSVLAPEVAEGVQKMLALSNARVIRSSILILPERSLTDLQ
                     VHRLVREADNPNRKYFTHPQHQYAWLSEVLTAPEKERLAQFLEIEAEPPASVKA"
     gene            1915..2232
                     /locus_tag="VFQ61_23540"
     CDS             1915..2232
                     /locus_tag="VFQ61_23540"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9957501.1"
                     /translation="MKHLLYAYRVLLSGIHLMRIGEVVANIGVLNEIFRSPPIDELVA
                     RKRSGAEKMLLEESELEQHSTLLDALERELDRAHDQSRLPEAPTTIVALDALVVRARL
                     SAA"
     gene            2385..>2667
                     /locus_tag="VFQ61_23545"
     CDS             2385..>2667
                     /locus_tag="VFQ61_23545"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9957502.1"
                     /translation="MKLPKSRGDADRKTRPERVQLTGAALDELTARVQKHFSENKPEP
                     ASFPAMASRVIDLAEHPDVDVARLAHLIERDPAICAAVLAVANSAANRRS"
BASE COUNT          548 a          828 c          807 g          484 t
ORIGIN      
        1 ttgagctctg cgcccaccct acccagactc acttcgatct cgcttgttcc gaatgagtac
       61 gcttggcatg ggttctgctt agagcgcgcg acgagagtcg tgggtcgggg ccttggaggt
      121 ttgcaaggaa tgcccccgac gacctacgcc ctgggagacg gatgaaatcg gaacagcgtt
      181 tggcgctcga tatgcgcggt cgttcgaaac gaggatgcaa ccccgaccgc acactgcaac
      241 ctgacgcgca cgaggtgcat gtgctcgttt acgaacacgg cacgctaggt tccggcgagc
      301 ggttgccgag ccccgatcgc tcgctggagt tgacggtact cgctcagagt cagggggaat
      361 cacctgcgga gttcacccaa cgcgtgctcc gccaagtccg agctctgaat gcttctggcc
      421 gtcgcatccg caaagccggc atcgtattga actcgggcca cagtacagag gcgatgcagg
      481 cgcgagagat gatcgggcgc agcgtgttga accggtttcc cgaagatcat tcagggagcg
      541 gcgcacaggg cggcagcggc cccgaaagct gggcggaatt cgtggccccg ggcgccggag
      601 aaaccagccc agaatatcgt tatcagctgt tcaatctggt tgaccaattg atggcgagcg
      661 caccagattc tcaaaccgcg gtgcggatcc gattccgccc gagcgagctc agccaggagc
      721 tcgctcgggt accacgggtt gcctgagagg gagtgttagc gatacgtgcg caagcgcgcc
      781 aaacgcatgg cgcgccgatt ccacgctggt gccaccacgc caacacaggc cgcgaagtag
      841 accagcgtga gcaccagcgg gaagctgtac gcgatgtcgg gggctcgccc gaatacgcgc
      901 gctagcacgc ccaggatcac ggatcccaaa ccacccaggg cggttcccac gatcagcgcg
      961 aacagcgcct gccgacgcca atcgaagcgc tcgtcctgag cgggacgcag gaacaacagc
     1021 ggcccccaat gcgcgtccgc gtcacacata tcggtaatca aagaatcact gcggtagtag
     1081 cggggtttca tcgtcactct tgcaaccgtc gaactgggag acggccctaa cctcgaggaa
     1141 ttaagattgg cgctcagctc taggggtgat ggtgccccgc cgcgaagcac aaggcgcagg
     1201 cgggcaggta cctgagaaga ttcgtgctga ggcattccgc gccggcatgc ggcgcgattt
     1261 gtaacacttc agtcctcact tgcacctgcc gtctatctcg agcacatgcg cacgccgcca
     1321 tcctccgaag gaaatacctg cgaattccgc gtcggccgtc tgatggaaat tcgagtcccc
     1381 aacggctatc gaactgctga tgacgtcgat cgaatggtgg cccgcatgac ctcgctgagc
     1441 ctcagcttag caagatcaga aaaattcatt attgctgctg attggcgcaa ggtttcggtc
     1501 ctagcgcccg aagtggcgga gggcgtgcag aagatgctcg cgctatcgaa cgcgcgggtg
     1561 atccgcagct cgattttgat ccttcccgag cgctcactca ccgacctgca agtgcatcgg
     1621 ctcgtgcgtg aggcagacaa cccgaaccgc aagtacttca cccatcccca gcatcagtac
     1681 gcctggttgt ccgaggtctt gaccgcgccg gagaaagagc gcctcgccca attcctcgaa
     1741 atcgaggccg agccgcctgc ttccgtcaaa gcctgacgcc gaagcttcat ccacccgcgg
     1801 tccccgcacc tgtcgcctcg gagcaggtat gatgcacgac cgtgaaagca tcccaactcg
     1861 aggaagtctg gagctggctg gatcgcgact atctgaggca agtcgaaggc gagcgtgaag
     1921 cacctgctct atgcatatcg agtcttactc tccgggatcc atttgatgcg gatcggagaa
     1981 gtggtcgcga acatcggcgt actcaatgag atctttcggt ccccacccat cgacgagctc
     2041 gtcgcacgca agcgctccgg agctgaaaaa atgttgctgg aggagtcgga gctcgagcaa
     2101 cactcgactc tgctagatgc cctcgagcgt gagctggatc gggcccacga tcagagccga
     2161 ttgcccgagg cgcccaccac catcgtggca ctcgacgcac tggtggtgcg cgcacgactc
     2221 agcgcggcgt gagctcccga aatcgggtcg cgggtgagag cgctggtcac atgtatgtgg
     2281 cagctctggt cttcgcgttt ccttcgcaag gtcattgcca gtcggggaaa cctgcagtta
     2341 ggatctcgcc acggattcat tcgaagcgag ggatgcgggt gaccatgaag ctcccgaaat
     2401 cgcgtggcga cgccgatcgt aaaactcggc ccgaacgcgt tcaattgacc ggggccgcac
     2461 tcgacgagct caccgctcga gtgcaaaagc acttctcgga aaacaagcct gagcccgctt
     2521 cttttccggc gatggcttcg cgcgtgatcg atcttgcgga gcatcctgac gtagacgtcg
     2581 cgcgcttggc gcacctgatc gagcgtgacc ccgcgatttg cgcggccgtg ctggcggtcg
     2641 ccaactccgc cgccaatcgc cgctctt
//
LOCUS       DASTKU010000677         3345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1246237, whole genome shotgun sequence.
ACCESSION   DASTKU010000677 DASTKU010000000
VERSION     DASTKU010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246237"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFQ61_23550"
     CDS             complement(<1..413)
                     /locus_tag="VFQ61_23550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957503.1"
                     /translation="MATAWRSLERRCGWSQFFACSFDAPIGRTRPSQPSAKHDLLHRG
                     GMKFYAYYADVPPEVLGELVHTAKLARLVTAGGSGPHLGLDPFLPLDAGFELHLNRTD
                     EQISDLRESAACAIEIDEPLSVIPSHWIDPRTQASR"
     gene            545..1309
                     /locus_tag="VFQ61_23555"
     CDS             545..1309
                     /locus_tag="VFQ61_23555"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9957504.1"
                     /translation="MQARALVAIRYAVSVKPEAWEIPEVPVPEATAHDAAVLRIYLLL
                     SAWAAARGNCRVARNLALRWLENYPRTGIDPDVCVISPAPADFESLTSLRLWEPSRTP
                     PVFCAEVVSASHPYKDYSEIHERYAAAGVFELVVLDPGLFGPKALGGPVLLQLWRRDA
                     SGAFERLHFGDGPVFSQALDAWISVIAGQLLISSDQAGVDRWLTGEERALAEMERALA
                     EKERALAEKERALAAREEIERRLRELELSLERNRAP"
     gene            1716..2846
                     /locus_tag="VFQ61_23560"
     CDS             1716..2846
                     /locus_tag="VFQ61_23560"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9957505.1"
                     /translation="MQARRLLVTLSNLGTATIVSLCLSLSAACSGTNDGSGGATGSGG
                     KTGSGGSANGGTGTGGSANGGTGTGGSASGGASSGGSPSGGASSGGKTGSGGSASGGT
                     GMGGSSNGGSSNGGGSNGGSSNGGSSNGGATNKGGASAMGGGGGAAMGGSGNGGAPAG
                     GSGGAGSFDPCPASGACKVLPLGDSITFGIQYEGAWRVELFSKAVAAGKKITFVGSQM
                     NGPSMAGGQSFPKNHEGHSGYTIDQMKPYAMTTDVPYMPNMILLHIGTNDTYGSSPSG
                     APGRLSALVDLITSTYPNALLVVAKIVPYPAQAANVKLINDSIPALVTKNQMAGKHVI
                     MVDCFTGFTTSSMLSSDSIHPNKTGYDFMGDQFYAAVASYLH"
     gene            complement(3009..>3345)
                     /locus_tag="VFQ61_23565"
     CDS             complement(3009..>3345)
                     /locus_tag="VFQ61_23565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957506.1"
                     /translation="TTDQDVPAQLQGALDGAYISARGCNFALPSAPRPLDLNRVNLTL
                     TVGSGAPAIVPGVKDAAACGAAPGWFYQSSSVSPNSIVLCEASCSALKSSPNAHLDIL
                     TGCETKVSN"
BASE COUNT          613 a         1022 c         1122 g          588 t
ORIGIN      
        1 cgcgaagctt gcgtcctcgg gtcgatccag tgcgagggaa tgacgctgag cggctcatcg
       61 atctcgatgg cgcacgcggc gctttcgcgc agatcggaga tctgttcgtc ggtgcggttg
      121 agatgcagct cgaagccagc gtcgagcggc aagaaaggat cgagtccaag gtgaggcccg
      181 ctgccgcccg ccgtcaccaa gcgcgcgagc ttggcggtgt ggacgagctc gccgagcact
      241 tccggcggga cgtccgcgta gtaggcgtag aacttcatgc ctccacgatg cagtaaatcg
      301 tgcttggccg agggttgtga cggtcgcgtg cgcccaattg gcgcatcgaa cgagcacgca
      361 aagaactgcg accatccaca acgtcgctcg agcgagcgcc acgcggtcgc cactcgtcgc
      421 cactgttggt gagcgcacgc gattctggaa ctgcgtaaat tcgctgacga aaggagctgc
      481 tcaggaggag gcatgggcgt tgctgaaccg aggctgcccc atgacagcta tacttcgccg
      541 ggtcatgcaa gcgagagctc tagtcgcgat tcgctacgcg gtctcggtga agcctgaagc
      601 gtgggaaatt cccgaggtgc ccgtgccgga ggcgacggct cacgatgcag cggtgctgcg
      661 aatctacctc ttgttgagcg cctgggcagc cgcgcgcggc aattgtcgcg tggcgcgcaa
      721 cttggccttg cgctggctcg agaactaccc gcgcaccggg attgatcccg acgtgtgcgt
      781 gatttcaccc gcgccggccg acttcgagag cctgacctcg ctcaggctat gggagccttc
      841 gcggacgcct ccggtgtttt gcgcggaggt cgtgagcgcc tcgcatccat acaaggacta
      901 ctcggagatt cacgaacgct acgccgcggc gggtgttttc gagctcgtgg tgctcgaccc
      961 gggcttgttc ggtcccaaag cgttgggcgg gccagtgcta cttcagctct ggcgtcgtga
     1021 cgcaagcggg gcgttcgagc gcctgcactt tggggatggt ccggtgttta gccaggcttt
     1081 ggatgcttgg atttcggtca ttgccgggca gcttttgatt tcgtccgacc aggccggcgt
     1141 cgatcgctgg ctcaccggtg aggaacgggc gctcgccgaa atggaacggg cgctcgccga
     1201 aaaggaacgg gcgctcgccg aaaaggagcg ggcactcgcg gcgcgcgaag agatcgaacg
     1261 gcgcctgcgc gagttggagc tcagcttgga acgaaaccgc gcaccgtagc tccactcttg
     1321 tcgggatcct tgcggcgtcg agcgcttgtc acaccgcctc gctgacgacg ccgtcggttc
     1381 gtggtgcatt cggagacatt gatcggctcc gatgtagccc gcgcgattgg atcgccgggg
     1441 gcgtaggtcg cggaagcttg ccggtgtccg cgcgctgcgc tcggagctcc gagctcggag
     1501 cgacgacgca ttcgaccgtg tgacgttcga cgagctggag gagttacggc gcgacaacgc
     1561 ttgccgcgat gcggtcgctc actcgcactt tcgcgtcgag ccacaacttc acagccactc
     1621 gagaacaact gggggatggc taaattgacg ctacctcgcg cacctcgcac gcagttgaat
     1681 ggtccgcctt ctccccacac ggagcgtaaa gcccaatgca agcgcgtcgc cttctcgtca
     1741 ctttgagcaa ccttggtacg gcgacgatcg tctcgctgtg cctcagcctg agcgctgctt
     1801 gcagcggcac gaacgacgga agcggtggcg cgaccggctc cggaggcaag actggctccg
     1861 gaggcagtgc caacggcggt accggcacgg gaggtagcgc caacggcggc acgggcacag
     1921 gaggcagtgc aagcggaggt gccagctccg gtggcagtcc aagcggtggc gccagctccg
     1981 gagggaagac cggctctggc ggtagcgcca gcggcggcac cggcatggga ggcagttcca
     2041 acggcggcag ctccaacggc ggtggctcca acggcggcag ctccaacggc ggcagctcca
     2101 acggcggtgc gacgaacaag ggcggcgcgt cggctatggg cggtggcggc ggcgccgcaa
     2161 tgggcggtag cggcaatggc ggagctcccg cgggaggaag cggcggcgca ggaagcttcg
     2221 acccttgtcc cgcgagcggt gcctgcaagg ttctgccgct cggcgactcg atcacgttcg
     2281 ggattcagta cgagggcgcc tggcgcgtcg agctctttag caaggcggta gccgcgggca
     2341 agaagatcac gttcgtcggc agccagatga acggtccttc gatggcaggc ggccagagct
     2401 tcccgaagaa ccacgagggt cacagcggct acaccatcga ccagatgaag ccgtacgcca
     2461 tgaccaccga cgttccatac atgccgaaca tgatcctgct ccacatcggc acgaatgaca
     2521 cctatggcag cagcccttcg ggagcacccg gccgtctttc cgccttggtc gatctgatta
     2581 cgagcacgta tccgaacgcg ctcctcgtgg tggcgaagat cgttccctac cccgcgcaag
     2641 cagccaacgt gaaactcatc aatgacagca tccccgctct ggtcaccaag aaccagatgg
     2701 ctggcaaaca cgtcatcatg gtggactgct tcacgggatt cacgacctcg tcgatgctga
     2761 gctcggactc gatccacccg aataagaccg ggtacgactt catgggcgac cagttctacg
     2821 cggcggtcgc gagctatctg cactgagtcg gattaagcga cgtctggatt cgcgctcgca
     2881 atgtccggac ggtttcacct aacttagctc cgagctactg catcgcagcg ataccattgg
     2941 cgcaccttgg tgcagcgctc caagggctga ttgcggacgt tgtccgagac acagctacca
     3001 gcccccaatc agttggaaac cttggtctcg cagcccgtga gaatgtcgag gtgcgcgtta
     3061 ggtgaagact tgagtgccga gcagctcgcc tcgcagagca cgattgaatt cgggctgacc
     3121 gaagatgatt ggtagaacca acctggcgcg gcaccgcagg cggctgcgtc cttgacgccg
     3181 ggcacgatgg cgggagcgcc gctgcccacc gtcaatgtca ggttgactcg gttcaggtcg
     3241 agtgggcggg gggcgctcgg caaagcgaag ttacatccgc gcgcggagat gtacgcgccg
     3301 tcgagcgcgc cttgtaactg cgccggaacg tcttgatccg tggtg
//
LOCUS       DASTKU010000678         3095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_576252, whole genome shotgun sequence.
ACCESSION   DASTKU010000678 DASTKU010000000
VERSION     DASTKU010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576252"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            460..1989
                     /locus_tag="VFQ61_23570"
     CDS             460..1989
                     /locus_tag="VFQ61_23570"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9957507.1"
                     /translation="MHARRDGSSLWLSALMAGTLVTAACSSSNEPGAAPAAGGANGGT
                     SGAGAATGGSLGGQNPSTTAGRGGSTSAGGASGASAGGTSGGVAGGAGSSNGGKTASG
                     GGSTSGGSPATGGSTARGGASAGGAPSMGGSGSTMPDSTDGKVFFDGTKLHQIDIVVE
                     SAHLGDLDSDTDNRVPCKFTYDGVSIENAGIRRKGQSTLQPLAEKPSFSIKLDEFVMG
                     ADIDGVQKFALNNTVKDPSYASEVVAYETYRRAGVPAPRTAHAVVRFNGTLKGLYVVS
                     EAINKRFLKHAFGNGDGNLYEGPWDFDKDVEQADLKDIEDGRKRDDLAALKRAITSAG
                     DAQLWDAIEPLMDANEMLTTVAVEMAANLWDGYAVAAWNYYLYNVPGGRFVMLPHGAD
                     WPYWYYQGDTARSTARLNPMDVDYRPWGEGSPSGILAVRLVAGNPDRYRAELSRVARE
                     AFVPAALEAELDRVDRVVHQVDTKQTLLASTVREFEPAIKIAHQFIKDRSAYLASQGF
                     K"
     gene            complement(2051..>3095)
                     /locus_tag="VFQ61_23575"
     CDS             complement(2051..>3095)
                     /locus_tag="VFQ61_23575"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9957508.1"
                     /translation="YISPEQVKNPDLVDQRSDVYSLGVTLYQLCTGQVPFGGQNHFSI
                     MMAHVTERPRPPSELRPDIPAALEQLILDALAKEPGQRPQSCAAFKARLEAALGDNRP
                     HPNQDGEQKLPSTRRSSQGSVMHLVPAGPFLMGAARREVYLDAFYIDRLPVTNREFLT
                     FLEVTGYQPVDPEAARFLHHWAGGKLPARQENHPVIYVSWGDAQAYASWAGKRLPTEA
                     EWEKAARGSDGRKYPWGRSEPGPALALYDKPDGGTVGVGSFPRGASPYGVLDLAGNVW
                     EWCADYDDPAFYERGPLHNPRNEQPGSRVLAVVRGGSYLCGPRALRTYARRSFEANYR
                     FGDGGFRCAQSAE"
BASE COUNT          610 a          958 c          989 g          538 t
ORIGIN      
        1 tcgaagagcc gtaattagac cgcccctgcc attccctcca gtggacacca atacacatgg
       61 cgaatcgacc gccgaatttg ttcccattca tggccttcgg cgagcgcctt caccgcttcc
      121 aaggagaacc gtgcccgaga agtaggcaac cgacgaaggg gcagctgagg cacgcgctct
      181 gcagaagtcg gttagtcccc gtttggtgaa cgtcggagaa acagcaacac aactaggtgt
      241 cgaggcgccg tgtctaccgt cgcgaccagg tgctgacttc gagcagcgta tgactttgta
      301 tgcatgatcg cagtcccgcc gtaggcgagc ggccttgcgc gaggggccga gcggtgctcc
      361 ctttgctgga tgtgtcgatt cgggttgaag cgcggatctg agttcgagtc agcggagcat
      421 ctcgttttgg agcgaacctc ggctgagaga aacccgggga tgcatgcccg cagagacgga
      481 agctcgctct ggctgagcgc tctgatggca ggcacgttgg tcaccgcggc ctgcagctcg
      541 tcgaacgaac caggagccgc tcccgccgcg ggcggcgcaa acggcgggac ttcgggcgcg
      601 ggcgcagcta cgggcggtag cctcggcggg caaaatccgt cgaccacggc gggccgcggc
      661 ggaagtacga gcgcgggtgg cgcatccggt gcgagtgctg gcgggacctc gggcggggtt
      721 gcgggtggcg ctggctcgag caacggcgga aagacggcca gcggaggtgg cagcacgagc
      781 ggcgggagcc cggcaacggg aggaagcacg gcccgcggcg gcgcaagcgc aggcggtgcc
      841 ccgagcatgg gcggcagtgg atcgaccatg cccgactcga cagatggcaa ggtgttcttc
      901 gacggcacca agttacatca aatcgatatc gtggtcgaaa gtgcacactt gggtgatctg
      961 gattcagaca ccgacaatcg ggtaccgtgc aaattcacgt atgatggcgt gagcatcgaa
     1021 aatgcgggca tccgtcgcaa aggccagagc acgctgcaac cattagcgga gaaaccgtcg
     1081 ttcagcatca aactcgatga gttcgtaatg ggcgccgaca tcgacggagt tcagaagttt
     1141 gcgctgaaca acaccgtcaa agacccgtct tatgcgtccg aggtcgtcgc ctacgagacc
     1201 taccgccgcg ccggggtacc cgcgccccgc actgcgcacg ccgtcgttcg cttcaatggc
     1261 acgctcaaag ggctgtacgt cgtctccgag gccatcaaca agcgcttcct caagcacgcc
     1321 ttcggcaacg gggatggcaa cttgtacgag gggccgtggg acttcgataa agatgtcgag
     1381 caggcggact tgaaagatat cgaggacggt cggaagcgcg atgatctcgc ggccctgaag
     1441 cgcgccatta ccagcgccgg ggacgcacag ctttgggatg cgatcgagcc gctgatggat
     1501 gcgaacgaaa tgctgaccac ggtggccgtg gagatggcgg cgaatctctg ggacggctac
     1561 gccgtcgcag cctggaacta ctacttgtac aacgtgcctg gcggtcgctt cgtcatgctg
     1621 ccgcacggtg ccgattggcc ctattggtat tatcaagggg acacggcccg cagcacggcg
     1681 cgactgaatc ccatggacgt cgattatcga ccgtggggcg aagggagccc gagcggcatt
     1741 ctcgcggtga ggctggtagc cggaaacccg gatcgctatc gcgccgagct gtcgcgcgta
     1801 gcgcgagaag cgttcgtccc cgccgccttg gaagcggagc tcgatcgagt agaccgcgtg
     1861 gtccatcaag tcgacaccaa gcagaccctc ctcgcgagca ccgtgcgtga gttcgagccg
     1921 gccatcaaaa tcgcgcatca attcatcaaa gatcgctcgg cctacctcgc cagccagggc
     1981 ttcaagtgag ccgtagctgc gcccgaagcc tgcgaagctt cacactccgc tgctcccccg
     2041 ctgaaaacgc tcattcggcc gattgcgcgc aacgaaaccc gccgtcaccg aatcggtagt
     2101 tggcttcgaa gctgcgtcgc gcataagtgc gaagcgcgcg cggaccgcac agataggacc
     2161 cgcctcgcac cacggcaagc actcgagacc ccggctgttc gtttctcggg ttgtgtaatg
     2221 gccctctctc gtaaaaagca ggatcatcgt agtcggcgca ccactcccaa acgttgcccg
     2281 ccagatccaa cacgccatat ggcgaagctc ccctcggaaa gctgccgacg cccaccgtac
     2341 cgccatcggg cttatcatag agtgccagcg ccgggccggg ctcactgcga ccccaggggt
     2401 acttccttcc gtcgctgccg cgcgccgcct tctcccattc cgcttctgtg ggtagccgct
     2461 taccggccca gctcgcgtat gcctgcgcat ctccccagga cacgtaaatc acgggatggt
     2521 tttcttggcg cgcggggagc ttgccgcccg cccagtgatg gagaaagcgc gccgcctccg
     2581 gatccacggg ctgatacccg gtcacctcca agaaggtcag aaattctcga ttcgtcactg
     2641 ggagtcggtc gatgtagaac gcgtccaggt agacttcgcg ccgagccgca cccatcaaaa
     2701 aggggcccgc cggaaccagg tgcatcacgg agccttgact gcttctacgc gtcgagggca
     2761 gcttttgctc gccatcctgg ttgggatgcg ggcgattgtc tcccaacgcc gcctcaagcc
     2821 tggctttgaa ggccgcgcag gactgcgggc gctggccggg ctctttcgcc aacgcatcta
     2881 gaatcagctg ttcgagcgca gctggtatgt ccggtcgcag ctcggagggg ggtcgtggac
     2941 gctcggtcac gtgagccatc atgatcgaga aatggttctg gccaccgaac ggcacctgtc
     3001 ccgtgcagag ttggtacagc gtcaccccga gcgaataaac atcggatcgc tggtcgacca
     3061 gatcgggatt cttgacttgc tcgggcgaga tatag
//
LOCUS       DASTKU010000679        14981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_131334, whole genome shotgun sequence.
ACCESSION   DASTKU010000679 DASTKU010000000
VERSION     DASTKU010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14981
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131334"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1319)
                     /locus_tag="VFQ61_23580"
     CDS             complement(39..1319)
                     /locus_tag="VFQ61_23580"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9957509.1"
                     /translation="MPGHIRANECAPRIVEASDSAASLGITSVRLTGNDGTTNRRATD
                     REPASVLLVDDDPTVLRALDRTLKSRGYEVVTAPDGEEATRQIGQRAFDVIVSDIGMP
                     GIDGIQLLREVREHDLNVPVILITGEPAVRTAVQALEYGAFHYLTKPVENEALESVLD
                     RAVRVHRMARIKQQAAELLGNANALGADRAGIEASFDRALQLMWMAYHPIVDPRTQTI
                     FGYEALLRTQEPSLPHPGAILDAAIRLDRLEELSRLIRARAAEPMLTNESSGKLFVNL
                     HVTDLLDPLLLSPDSPLFKIASRVVLEITERTSLDEVRDVRTRIARLRELGYSIAVDD
                     LGAGYAGLTSFTLLEPEIVKLDMSLIRDVHQNSTKQKVVRSMAGLAKDMGMIVVAEGV
                     ENTSERDVLLELGCDYLQGFLFARPGPAFPEVKW"
     gene            complement(1391..1882)
                     /locus_tag="VFQ61_23585"
     CDS             complement(1391..1882)
                     /locus_tag="VFQ61_23585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957510.1"
                     /translation="MTPRSPKPPVRRVDEASASTDPTSLEPSVSAPPAATTQAPFEAV
                     DVVEAEHIVARGTRRTAWFVRHNSGWLRAQQVEGAIVITLACGPGTVWLRRIELKLRR
                     GTPLMCVVSQPRSQIEKSAKSPLAYLEKGHSLPKPLVQRLEYRVARGGHLERTPTDSK
                     KRG"
     gene            complement(1879..2352)
                     /locus_tag="VFQ61_23590"
     CDS             complement(1879..2352)
                     /locus_tag="VFQ61_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9957511.1"
                     /translation="MKAGLLVKGAYTIIREVGRHILRRPVVGVAAAARTPDDRWLLIR
                     RGDSGQWALPGGTLEWGETLESALRRELLEEAGVEQLNVVRLVGVYSRPDRDPRFHAV
                     TVVVEVQVDAPTRQPTNPLEILEVRLFERSALPNELSHNMTPMLEDALVGKSRFE"
     gene            2670..4130
                     /locus_tag="VFQ61_23595"
     CDS             2670..4130
                     /locus_tag="VFQ61_23595"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9957512.1"
                     /translation="MASARIDAARIDAAQTDGASIDTANDAAPSGTPDAQLGRVSDNL
                     PFTPSGLRLGSIALRTWIYSDTGPKRTRYGYLRAGSIVDARGPAMKNEGCPGGWYRIN
                     PRGFVCVGMGATLELNHPVLVAANIRPVRKAGLPYLYAKAGKTPPYFYFRLPSVAQMQ
                     ASEGERVTARAAVFRERALASGLKSEPPPEFLQGGRKLEKPYGSKEGLHFSAHSGQAA
                     PDSGFSITRVIDWQNRLFGLTTELELLALDRTKLVVPSQFHGVELGPDEGLPVGFVEP
                     RFAPKYVLNSRAELVQQGAFEYREGLKLTGAHKPGAMLEAAGGVWVPESVLRKIAPRT
                     SFPSVATGDRKWIDISIRDQTLVAYVGRRPVYVTLVSTGRGGMGDPETTLSTVRGSFM
                     IYQKDTSSTMDGEQDSAADSYALRDVPFVQYFHKGFALHGTYWHDEFGRVRSHGCVNL
                     SPVDAAWLFEWTDPSVPPDWHGVINKDRGTVVFIRP"
     gene            4311..5552
                     /locus_tag="VFQ61_23600"
     CDS             4311..5552
                     /locus_tag="VFQ61_23600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957513.1"
                     /translation="MGTSNTHPGGEEMKSSVLDPLEALFSEREREITDRRQRDLAGLR
                     TLVGAGVLVGLTSLGLYAAQFGSWGVFARVLSGGVLTCAAAALIGVVGGFLFGLPRPL
                     VVEEHAGSEGPSSQENPGRAGYVLTTNLERISDISIKILIGLALTQLYRVPELLGRFS
                     DWLGRNVPEHSEAGKVAVGAMGYALCLGFLSGYLWTRLVLSPAFRRADEQLQKTLQRL
                     AAAERKVREAGARVESMEMCSESKLSAAEQSAKSRIEGAERAAETILSEARAKASAAE
                     QAEKRRLETLNQTLRDMLHALHAPAGQRGFDRAIAVAQQYVDSYGVPDCSLFQVRCAA
                     AYGRRATELPETYDDARGLALEAVRRAIDASPELGRYWLSMWFHPEHPHATPGEDWLQ
                     VFFDEGEFQNLIGRDPYVAPE"
     gene            5616..6857
                     /locus_tag="VFQ61_23605"
     CDS             5616..6857
                     /locus_tag="VFQ61_23605"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HET9957514.1"
                     /translation="MRRLSDWQIDTDRFRTRMFPELMRRKSERMCRSPLAFLRGAAPL
                     FYLLLASIPELEAGPAGEGWLVGDAHLENFGAFQVERRSEEENGKREVVFDLNDFDEA
                     VVGPLRWDVMRLTTSLVLGARELGIDGPSVLRLCERLIAHYVETSLRGAPLPQVPAPV
                     SALLERARTRSKKDLLEARSSGNGRERRLIRSEGYRELPPEIAARVPTTLLQYAGTLS
                     AEEQPKQEQLQVMDVAHRVAGTGSLGCLRIAALTRGKSEGDGAWLFDLKEQVEPSALL
                     VSNAARDAAPRPSPLDASGLAERPAQRVLRASKACLSRPPRMLGFAELEGRSMLIRRL
                     TPQEDKLELARLRSAELPELSAYLGAVLGAAHARGRMQATPAAWSCAEQRELLERAVL
                     CAGLHEACYLEFCLTAHPSRS"
     gene            complement(6969..7994)
                     /gene="hypE"
                     /locus_tag="VFQ61_23610"
     CDS             complement(6969..7994)
                     /gene="hypE"
                     /locus_tag="VFQ61_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HET9957515.1"
                     /translation="MSSCPIPSESEIVLLAHGGGGRLTRKLIQELFWQELGEPTDGRR
                     HDGVVLESRPGRPVLTTDAFVVRPLFFPGGNIGELAVYGTVNDLAMCGARPTELTASF
                     ILEEGLPLAVLRDVVRSMKQAADRSGVKIVTGDTKVVERGHGDQLFICTTGLGWVEPN
                     VRVGPEQIAVGDQIIVSGDLGRHGIAVMATREGLTLSGDIQSDCQELTSQVQALLGAG
                     IELHCMRDLTRGGLGAALIEIAQDARLQLNIDSGSIPLSAAVLGAAELFGLDPLLLPC
                     EGRFAAFVPQSQLETTLAVLPSPFKARIGEVVQIEAARGKVVLETELGTQRELELPLG
                     EQLPRIC"
     gene            complement(7994..9085)
                     /gene="hypD"
                     /locus_tag="VFQ61_23615"
     CDS             complement(7994..9085)
                     /gene="hypD"
                     /locus_tag="VFQ61_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HET9957516.1"
                     /translation="MKYVTEFRDRRLAESLIQRIHETVTRPWTLMEVCGGQTHALLRF
                     GIDSMLPKQLELLHGPGCPVCVTPLEAIERALRIAREPNVIFCSFADMLRVPGDSGDL
                     ALARAQGANVRLVYSPLDALLLAEQNPDKQVVFFAIGFETTAPANAFAVSEAKRRGLS
                     NFSLLVAHVRVPPALEALLTTPGRSLHGFLAAGHVCSIMGTREYERLSAAHRVPIVVT
                     GFEPIDLLQGILHCVQQLERGRYEVENQYARVVASDGNTTARALMESVFEVVPRRWRG
                     LGEIAESGLGLRAEYASFDAERRFVTDLQAIEEPADCISGLVLAGRKKPTECVHFGKG
                     CSLERPLGATMASSEGACAAYFHYRRGNA"
     gene            complement(9082..9324)
                     /locus_tag="VFQ61_23620"
     CDS             complement(9082..9324)
                     /locus_tag="VFQ61_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HET9957517.1"
                     /translation="MCLAVPGEIVAIEDGEPRHARVAFGAVERRVCLSLVPEAQVGDY
                     VIVHVGFAISRLDPEQAHLTLATLNEVESSQGTDDS"
     gene            complement(9350..11728)
                     /locus_tag="VFQ61_23625"
     CDS             complement(9350..11728)
                     /locus_tag="VFQ61_23625"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HET9957518.1"
                     /translation="MKKRWAIEVCGTVQGVGFRPYTARHAQALGLSGYVCNSGGGVRI
                     EAQGDVELLEMFRQRLLRIPSPARIRSLEHWEIQAEPCSEGGFAITRSRQTTEAGGPS
                     QPGLPPDLMVCADCLRELVDPEDRRYGHPFISCVACGPRYSVALGVPYDRAKTTLASF
                     PLCPACEREYRDSTQRRYHAETIACPHCGPQLEYRTRADSVPVIGNLALGAAIECLRR
                     GEILALRGLGGFQLLVDARSSRAVQRLRERKQREEKPLAVLFPSLFSAEQHCELTPAD
                     RRLLSSAEAPIVLVPRRTKAELAFEVAPLLDEVGALLPTTPIHFLLLQALGFPVVCTS
                     GNPSGEPLCTRNEEAFLRLREVADGFLIHDREIARPLDDSVARAYPSETSDVCQSTPS
                     NRANDSATAAPDLGEDRIELLRRARGYAPREVARLPAGAAMLSLGAHQKSSVALCHEG
                     RAFLSQHLGDLDSVPSRENFRSTVRDLLAYHGAVPQTVVCDLHPDYASSLEAETLARE
                     FQARLVRVQHHQAHVAAVLAEAGANPHDACFGLAWDGTGYGTDGTSWGSEAWLVNDGR
                     FEHFAALDGFALTGGDRAAREPARAALGVLFACSYELAESYAARQFGARGQLLLEAQG
                     RERMTNITRSIGRLYDAVAGLLGIRARCAYEGQSAIELEVLARRASEPSVYPLPWQPP
                     AEGRSARGDFRPLIRQICADHARGVDPARIAAGFQESLVNFGLELARRADRETIVLSG
                     GCFHNRFLRGELTRRLRRAGHRVLLPQRVPGNDGGIALGQLWIASLSEKSAS"
     gene            complement(11865..13502)
                     /locus_tag="VFQ61_23630"
     CDS             complement(11865..13502)
                     /locus_tag="VFQ61_23630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET9957519.1"
                     /translation="MLKRWLLLIGTVLATASPAWAQEPSPAPAVASANAPATASAATS
                     AAAAPSAEANSAPSAAASAVAPTTAAAPTAAAVPAAAPVAAMQAAPAPSAGPTPAEIE
                     KRLLDLEAYVNNGARADATTSKVSGPGPGHNGWMMVCSALVLFMTLPGLALFYGGLVR
                     QKNVLSVMAQCMGLAGMVTVLWWAFGYSLSFAPGGPMIGGTKFLFFNGVDAIPNTDYG
                     AWVSHNVFSIFQLMFAIITPGLIVGAIAERMKYGSLMLFLLGWMLIVYFPLAHMVWGV
                     DGMMNGVWNAKASIKAIDFAGGTVVHMSSGWSAITLAVILGKRKGFGHENFAPHSLVL
                     TMIGTGMLWVGWYGFNAGSAVGADGIAANAFVTTTIAAGVAGASWPAVEWMTRGKPTV
                     LGFCSGIVGGLVVITPGAGFVTPSAAVIIGVLAGVIPYFACTKLKAMLGYDDALDTFG
                     VHGVGGTLGALLTGFFAKAEVNPNLNTNLSSVVGSTLWIEQLKAMAFTVVLSVVATCV
                     LAFAVRAVLGLRPSVEDEETGLDLTDHGERGYHLGET"
     gene            13854..14552
                     /locus_tag="VFQ61_23635"
     CDS             13854..14552
                     /locus_tag="VFQ61_23635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957520.1"
                     /translation="MALTPACNKKDPAACEQAQQTIRQALDREQFQAITPWREYAWRQ
                     CEDRTALAALDQKIVARQNEVQARNRAAEARRSETAQLLRVFLSWVAETRAAPDRATA
                     TPVCDPPAPNDPEKEKSKQRMCTASRTVQGPSGMRPLSVRYFAAEPTAARFIINLPDA
                     TSCEELGAIKSKTWQVATTSGSTTARFSCDFTSGPLSGMRAVLSQAVNADLHVFNPTY
                     LDKEPQYRAILDSP"
BASE COUNT         2683 a         4721 c         4805 g         2772 t
ORIGIN      
        1 ggagttcaat gtgccgggct tgaactgatc catgcggctc accacttcac ctcggggaag
       61 gccggacccg ggcgcgcgaa caggaagcct tgcaggtagt cgcagccgag ctcgagcagc
      121 acgtctcgtt ccgacgtgtt ctcgacgcct tcagcgacga caatcatccc catgtctttg
      181 gcgagacccg ccatcgaacg caccaccttt tgcttggtgc tgttctggtg cacgtcgcgg
      241 atcaacgaca tgtcgagctt gacgatctcg ggctccagca aggtaaagct cgtaagaccc
      301 gcgtagcccg cgccgagatc gtcgacggcg atgctgtaac cgagctcgcg tagccgtgcg
      361 attcgcgtgc gcacgtcgcg tacctcgtcg agcgaagtgc gctcggtgat ttcgagcacg
      421 actcggctcg cgatcttgaa aagtggagaa tctggagata gcagcagcgg atccagcaga
      481 tccgtcacgt gcaagttcac gaacagcttg ccgctggact cattggtgag catgggctcg
      541 gccgcgcgag ctcggatcaa tcggctcagc tcttccaaac gatcgagccg aatggcggcg
      601 tccagaatgg cacccgggtg gggcagagag ggttcttggg tacgcagcag ggcttcgtac
      661 ccaaagatgg tctgagtgcg cgggtcgacg atcgggtggt acgccatcca catcagctgc
      721 agggcgcgat cgaagctggc ctcgatgccg gctcggtctg ctcccagcgc attggcgttc
      781 cccagtagct ccgcggcttg ctgtttgatc cgcgccatcc gatggacccg caccgcgcga
      841 tccaacacgc tctccaaggc ctcgttctcc accggcttgg tgagatagtg gaaggcaccg
      901 tactccaggg cttgaacggc cgtgcgcact gccggctccc ccgtgatcag gatgacgggc
      961 acgttcaggt cgtgctcgcg aacctcgcgc agcagctgga tcccatcgat tcccggcatg
     1021 ccaatgtcgc tcacgatgac atcgaatgcg cgttgcccga tttgccgagt cgcctcttcc
     1081 ccgtcgggcg cggttactac ttcgtagccg cggctcttca gggttcggtc gagagcccgt
     1141 aacacggtag ggtcatcatc caccaacaac acacttgccg gctctcgatc cgtggcacgg
     1201 cgattcgtcg tcccgtcgtt gccggttagt ctgacgctcg tgatccccaa ggaagcggca
     1261 gaatccgatg cttcgacgat gcgcggcgcg cactcgttgg ccctgatgtg acctggcata
     1321 cctcccaatt caacggtcgt tcatcgcaaa ggtgtagggt ttgcgcgagg aggtctgcag
     1381 atcgttgcat ttagccgcgc tttttcgaat cagtgggagt gcgctccaag tgtccaccgc
     1441 gtgcgactcg gtactccagg cgttgaacta gcggcttggg taggctgtga cccttctcta
     1501 aataggcgag cgggcttttg gcgcttttct cgatttggga cctaggctga gacaccacgc
     1561 acatcagagg cgtgcctcgc cggagtttta gttcgatcct gcgcaaccaa accgtgccgg
     1621 ggccgcacgc cagcgtgatg acgatcgcgc cttccacctg ctgggctcgg agccatcccg
     1681 agttgtgacg cacgaaccaa gccgtgcgcc gcgtgcctcg cgccacgatg tgctctgcct
     1741 cgaccacgtc tacggcctcg aacggcgcct gcgtcgtagc cgctggagga gctgatacgc
     1801 tgggctcgag cgaggttgga tctgtggacg ccgaagcctc gtcgacgcga cggacaggag
     1861 gtttgggcga ccgtggtgtc attcgaagcg gctcttgccc actagcgcat cctcgagcat
     1921 gggggtcatg ttgtgggaga gctcgttggg taacgcgctg cgctcgaaca agcgcacctc
     1981 caaaatctcg agtggattgg tgggctgtcg tgttggcgcg tccacttgca cctcgaccac
     2041 gacggtcacg gcgtggaaac gcggatcgcg atcgggtctg gaatacacac ccaccaagcg
     2101 caccacattc agttgctcga cgccggcttc ttcgagcagc tcgcgacgca gtgcggactc
     2161 cagcgtctcg ccccattcca aagtgccgcc gggcagtgcc cactgacccg aatcgccgcg
     2221 tcgaatcaac aaccaccgat catccggggt gcgagcggcg gccgccaccc ctactaccgg
     2281 acgccgcagt atgtggcgtc cgacctcgcg gatgatggtg tacgcgcctt tgaccaggag
     2341 tcctgctttc acgcgcgcag gatgccgcgt tcgcgggcgt ctgtcgcatg gcttctgact
     2401 ccccacgcgt gcggccgtcg gtccgagctg cctcgagcgt gccagacact atgcctcgac
     2461 gacccttgac cgagcgtgtc gtggtatgag gcgcgccttc ggtgcgctct agagttctcg
     2521 tctttagtct gctgtgcgtc gcttcagggt gtgcgcggcg agagcctccc ggtgcgagtc
     2581 aaagggacca cgccgcgagc gcccctggtt ccccctcgtc ggttttgggg gatgcttctc
     2641 cgttagttcc ggggagcgtg tcgtcgccgg tggcctctgc gcgtatcgat gccgcgcgca
     2701 tcgatgccgc gcaaaccgat ggagcctcga tcgacaccgc aaacgatgct gcaccgagcg
     2761 gcacgccgga cgcgcagctt ggccgggttt cggacaactt acctttcaca ccctccgggc
     2821 tgcgcctggg cagcattgca ctgcgcacct ggatctatag tgacaccggc cccaagcgca
     2881 cgcggtacgg atatctgcgc gctggcagca tcgtggacgc gcggggcccc gctatgaaga
     2941 acgaaggctg tcccggtggt tggtaccgca tcaacccgcg cggcttcgtg tgtgtgggca
     3001 tgggtgcaac gctggagcta aaccacccgg tgctggtcgc ggccaacata cggccggtgc
     3061 gcaaagccgg gcttccttat ctctacgcga aagctggcaa aaccccgcca tacttctact
     3121 tccgcttgcc ctcggttgct caaatgcagg cgtcagaagg ggagcgggtc acggcgcggg
     3181 cggcggtgtt tcgcgagcgc gctctggctt cgggcctgaa gagcgaacca cccccagagt
     3241 tcctgcaagg cgggcgcaag ctcgagaagc cgtacggctc caaagaaggg ctgcacttct
     3301 ccgcccactc gggacaagcc gcgcccgata gtgggttctc gatcacgcgc gtcatcgatt
     3361 ggcaaaatcg gctgtttggg ctcacgaccg agctcgagct attggctttg gatcggacca
     3421 aactcgtagt gccgagccaa tttcatgggg tcgagctcgg tccggacgag ggcttgcccg
     3481 tcggcttcgt cgagccgcgt ttcgccccca agtacgtgct gaatagtcgc gcggagctag
     3541 tgcaacaggg cgccttcgag taccgagagg gcttgaaatt gaccggcgct cacaaacccg
     3601 gcgcgatgct cgaagctgca ggcggcgttt gggtgcccga gtcagtgctt cgaaagatcg
     3661 cgccgcgcac cagctttccc agtgtggcta cgggagatcg aaaatggatc gacatctcca
     3721 ttcgtgatca aaccctggtc gcgtacgtgg ggcgacggcc cgtctacgtt acgttggtca
     3781 gcacgggccg cgggggcatg ggtgatcccg agaccacgct gtccaccgtg cgcgggagct
     3841 tcatgatcta tcagaaagat acgagctcca cgatggacgg tgagcaagac agcgccgccg
     3901 attcctacgc tctgcgcgac gtgcctttcg tgcagtactt tcacaagggc ttcgcgctgc
     3961 atgggactta ctggcatgac gaattcggac gcgtgcgcag ccacggctgc gtcaacctgt
     4021 ctcccgtcga cgcggcttgg ctgttcgaat ggaccgatcc gagcgtgcct cctgattggc
     4081 acggcgtgat caacaaagat cgcggcacgg tcgtgttcat ccgcccctga gctcagtcac
     4141 gaatcgtctt tcgtaatctg ctccggcagg taacgtctgt tctctggtta gctgtttcag
     4201 ctcggtggtg atttcagtcg cgccgaaagg ccgaatggcc gaggtcggag ccgcttgacc
     4261 gccaacaccg cggttggtct tctgttcgct cctgaagaag ggcacgaaga atgggcacct
     4321 ccaacacgca tccgggtggc gaagagatga agtcgtccgt tctcgatccg ctggaggcgc
     4381 tgttctccga gcgtgagcgc gaaatcacgg atcgtcggca gcgcgatctg gctgggctac
     4441 gcacgctggt cggagcaggg gtgttggttg gtctgacctc tctcggccta tatgcagctc
     4501 aattcggcag ttggggagtc tttgcccgag tgctgtctgg gggcgtgttg acgtgcgcgg
     4561 cggccgcctt gatcggtgtg gtgggagggt tcttgttcgg tttgccgcgc ccgctcgtcg
     4621 tggaggaaca cgctggctcc gaggggccca gctcgcagga gaatccgggc cgggcgggtt
     4681 acgttctgac aacgaatctg gaacggatct ccgatatctc gatcaagatc ctgattggct
     4741 tggcgctcac gcagttgtat cgagtgccgg agttgctggg gcgcttttcg gattggctcg
     4801 gtcgcaacgt gcccgagcac tccgaagccg gcaaggtcgc cgtgggggcg atgggttacg
     4861 ccttgtgcct cggcttcctg agcgggtatc tctggactcg attggtgctg agccccgcgt
     4921 ttcgccgggc cgacgagcag ctgcagaaaa cgttgcagcg cctggcggca gccgagcgaa
     4981 aggtgcggga ggcgggagcg cgggtggaaa gcatggagat gtgctcggag tccaagctct
     5041 cggcggccga gcaaagtgcc aagtctcgca tcgaaggcgc ggaacgcgct gccgaaacca
     5101 tcttgtccga agctagggca aaggccagcg ccgcggagca ggcggagaag cgcaggctcg
     5161 agacgctgaa tcagacgctg cgcgatatgc tgcacgcgtt gcatgcgcca gccggtcagc
     5221 gcggcttcga ccgcgcgatc gccgtcgctc agcagtatgt cgatagctat ggggtcccgg
     5281 attgtagctt gttccaggtg cgctgcgcgg ccgcgtacgg ccgtcgcgcg accgagttgc
     5341 cggaaaccta cgacgacgct cgcgggctcg cgctcgaggc ggtgcgtcga gctatcgacg
     5401 ccagtcccga actcgggcgc tattggctgt cgatgtggtt tcaccccgaa catcctcatg
     5461 ccacaccggg tgaggattgg ttgcaggtgt tcttcgatga aggcgagttt caaaacttga
     5521 ttgggcgcga tccgtacgtg gctcccgaat agtcgatgac tccctcggcc ctcgaccctc
     5581 ggaaaatatg gaatccgctg tctgatgcac gcagcatgcg ccgcctgtcg gattggcaga
     5641 tcgacacgga tcgttttcgg actcgaatgt tcccggagct catgcggaga aagagcgaac
     5701 ggatgtgccg ctcgccgctc gcgttcttga ggggcgccgc tccgctcttc tatctcttgc
     5761 tggcgagcat tcccgagctc gaagcggggc ccgcgggcga gggttggctg gtgggtgacg
     5821 cgcacctcga aaacttcggg gcttttcaag tcgagcgccg ctccgaggag gagaacggaa
     5881 agcgcgaagt ggtattcgat ctgaatgact tcgacgaagc agtggtgggg ccgctgcgct
     5941 gggacgtaat gaggctcacg accagcctcg ttctcggggc gcgcgagctt gggatcgacg
     6001 ggcccagtgt gctccgcttg tgtgagcggt tgatcgcgca ctatgtcgag acgtcgttgc
     6061 gcggagctcc gttgccccag gtgccagctc cggtgtcggc cctactcgag cgggcacgca
     6121 cgcgctcgaa gaaggatcta ttggaggcgc gcagctcggg caacggtcgt gaacgacgat
     6181 tgattcgtag cgaaggctat cgcgagctac ccccggaaat cgcagctcgg gtccccacca
     6241 cacttctgca gtacgccggg acgttgtcgg cggaggagca acccaagcag gagcagctcc
     6301 aggtgatgga cgtcgcgcat cgcgtggcgg gtacggggag cttgggttgc ctccggattg
     6361 cagctctgac ccgaggtaag agcgaagggg acggagcatg gctcttcgat ctgaaggagc
     6421 aggtggaacc gagtgcgctc ctcgtctcga atgcagcacg ggacgccgcc ccgcgaccga
     6481 gcccattgga cgcgagtggc ctggctgagc gccccgcaca gcgcgtctta cgagcttcca
     6541 aggcctgttt gtcgcggccg ccacggatgc tgggtttcgc ggagctcgag gggaggagca
     6601 tgctgattcg acggcttacg ccgcaggaag acaagctcga acttgcgcgc ctccgttccg
     6661 ctgaattacc ggagctgtcg gcctatctcg gggcagtgtt gggggccgcg cacgcgcgag
     6721 gtcgtatgca agcaactccc gcggcgtggt cttgtgccga gcagcgtgaa ttgctggaac
     6781 gggcggtgct gtgcgccggc cttcacgagg cgtgttacct cgagttttgc ctaaccgccc
     6841 atcctagtcg gagttagtgg ccggcagccg ataacccgcg ttctgggccg aggtcgggcg
     6901 cttaagcgat gaagacgacc gcgcctgttc gttggcggtg ggcggcccca cttccgactc
     6961 gaatccgatc agcaaatgcg aggtagctgc tcaccgagcg gcagctcgag ttcacgttgg
     7021 gtccccagct cggtttccag cacgaccttg ccgcgtgcag cttcgatttg cacgacctcc
     7081 ccaattctcg ccttgaacgg ggatggcaat acggcgagcg tcgtctccag ttgcgattgc
     7141 gggacgaatg cggcgaaacg cccctcgcag ggcaacagca atggatccaa tccgaacagc
     7201 tcggcggctc cgagcaccgc ggcgctaagt gggatgctcc cgctgtcgat gtttagctga
     7261 agccgtgcgt cctgagcgat ctcgatcaac gctgcgccca ggccgccgcg ggtgagatct
     7321 cgcatgcagt gcagctcgat cccggcgccg agcagtgcct gcacttggct cgtgagctcc
     7381 tggcaatcgc tttggatatc cccactgagg gtgaggcctt cgcgcgtggc cattaccgcg
     7441 attccgtggc gccccagatc gccgctgacg atgatctgat caccaaccgc gatttgctcc
     7501 ggtccaaccc gaacgttcgg ctcgacccac ccgagcccag tcgtgcagat gaagagctgg
     7561 tccccgtgcc cccgttccac gaccttggtg tcgcccgtga cgatcttcac gcctgatcga
     7621 tcggcggctt gcttcatcga gcgcacgacg tcgcgtaaga cggcgagggg taagccttct
     7681 tccaggatga agctcgccgt caattccgtc ggtcgggcgc cgcacatcgc gagatcattg
     7741 acggtgccat agaccgccaa ttcgccgatg tttccgcccg ggaagaacaa cggccgcact
     7801 acgaacgcgt cggtggtgag taccggtcgc cccgggcgcg actcgagcac cacgccgtcg
     7861 tgtcgtcgac catccgtcgg ctctccgagc tcctgccaaa acagctcttg gatcagctta
     7921 cgagtgaggc ggcctccgcc gccgtgggcc agcagcacga tctcggactc gctcggtatc
     7981 ggacaactcg acatcaggca ttcccccgac gatagtgaaa gtatgcggcg caggcgcctt
     8041 cagaagacgc catggttgcg ccgagcgggc gctcgagact gcagccctta ccaaagtgga
     8101 cacactcggt gggcttcttg cgccctgcca gcacgaggcc gctgatacaa tcggcgggct
     8161 cttcgatggc ttggagatcc gtgacgaagc gccgttcagc gtcgaatgac gcgtattccg
     8221 cgcgcagacc caggccgctc tccgcgatct cgcccagccc acgccaacga cgtggcacca
     8281 cctcgaagac gctctccatg agggcgcgtg ccgtagtgtt cccgtcgctt gcgacgacgc
     8341 gcgcgtactg gttttcgacc tcgtatcgac ctcgttccag ctgttgaacg cagtgcagga
     8401 tgccttgcag gagatcgatg ggctcgaagc cggtcacgac gatgggcacg cgatgcgcgg
     8461 cggagagccg ctcgtactca cgtgtcccca tgatgctgca gacgtgcccc gcggcgagga
     8521 aaccgtgcaa actgcggccc ggtgtggtca gcagcgcctc tagcgcgggg ggcacccgaa
     8581 catgcgcgac caacaacgag aagttgctca ggccgcgccg cttggcctcg ctcaccgcaa
     8641 aggcatttgc gggtgcggtg gtttcgaagc cgatcgcgaa gaagacgact tgcttgtctg
     8701 ggttttgctc cgccaaaagc aaggcgtcga gcggggaata gaccagcctc acgttcgcgc
     8761 cttgcgcccg cgccaacgcc agatctcccg aatcacccgg cacacgcagc atgtccgcga
     8821 acgagcaaaa gatcacgttt ggttcgcgcg cgatgcgcag cgcgcgttcg atggcctcga
     8881 gcggtgtgac gcagaccggg caacccggcc catgcagcaa ttcgagctgc ttgggcaaca
     8941 tgctgtcgat gccgaagcgc agcagcgcgt gggtttgccc gccacagacc tccatcaggg
     9001 tccagggccg cgttaccgtc tcgtggatgc gttggatcag cgactcggcc aagcggcgat
     9061 cccggaattc agtaacgtat ttcacgaatc gtccgttccc tgcgaggact cgacctcgtt
     9121 caatgtcgcg agcgtgaggt gggcttgctc cggatccaag cgactgatcg cgaagccgac
     9181 gtggacgatc acgtagtccc cgacttgggc ttcgggcacg agcgagagac agacgcgacg
     9241 ctccactgcg ccgaacgcca cgcgcgcatg acgcggctcg ccgtcttcga tggcaactat
     9301 ttcacccgga actgcgaggc acatgagaat tgctttcgtg tcttgtcgtt caacttgcgg
     9361 acttctcgga gaggcttgca atccagagct gaccgagggc aatgccgccg tcgttaccag
     9421 gaacgcgctg tggaagcagc actcgatgcc ccgctcgccg tagccggcgc gtgagttcgc
     9481 cgcgtaagaa tcggttatgg aaacagccgc cgctcagtac aatcgtctct cgatccgcgc
     9541 gccgagcaag ttccagcccg aaattcacca ggctctcttg gaacccagct gcgatgcgcg
     9601 ccgggtctac gcctcgcgcg tgatcggcgc agatttgccg tatcaacggt cggaagtctc
     9661 cccgcgcgga tcggccctcc gcgggaggct gccatggcag agggtacacc gatggttcgg
     9721 acgcgcgtcg agcgagtact tcgagttcaa tcgcagattg accctcataa gcgcagcgtg
     9781 cccgaatccc gagcaacccg gcaaccgcgt cgtacaagcg cccgatggat cgcgtgatgt
     9841 tggtcatgcg ctcgcggccc tgcgcttcga gtaacaattg gcctcgagcg ccgaactgac
     9901 gcgctgcata ggactccgca agttcgtagc tgcaggcgaa cagcactccc agtgcggcgc
     9961 gcgcaggttc gcgcgccgcg cgatcaccgc ctgtcaacgc gaacccgtcg agcgctgcga
    10021 agtgttcgaa ccgtccgtcg ttcaccagcc aggcttcgct gccccacgac gtgccgtccg
    10081 tgccgtaccc agtcccgtcc cacgccaacc cgaaacaggc gtcgtgcggg ttggcaccgg
    10141 cctccgcgag cacggcggcc acatgagcct ggtgatgctg caccctcacc aagcgagcct
    10201 ggaactctcg ggccagagtc tcggcctcga gactggacgc atagtctggg tgaagatcgc
    10261 agaccacggt ttggggaaca gctccatgat aggccagcaa gtccctaacc gtgctcctga
    10321 agttctcgcg tgagggcacg ctgtccaagt cgcccaagtg ttggctcagg aatgcacggc
    10381 cttcgtgaca gagggcgacg ctgctctttt gatgcgctcc gaggctcagc atcgccgcgc
    10441 ccgcgggcag gcgtgcgact tcgcgtggcg cgtaacctcg cgcgcgccgc agcaactcga
    10501 tgcggtcctc tccgaggtct ggagcagccg tggcgctgtc gttcgcgcgg ttgctcggcg
    10561 tgctttggca aacgtcggag gtctcgctcg gatatgctcg ggcgaccgag tcatcgagcg
    10621 gccgcgcgat ctctcggtca tgaatcagaa agccgtccgc gacttccctc aagcgcagga
    10681 acgcctcctc gttgcgtgtg cagagcggct cccccgaagg gttgccgctc gtgcagacca
    10741 ccggaaaccc gagcgcctgc agaagcaaga agtgaatcgg tgtggtgggg agcagcgctc
    10801 ccacctcatc gaggagtggc gcgacctcga acgccaactc cgctttggtt cggcgtggca
    10861 cgagcacgat cggcgcctcc gctgagctca gtagtcgtcg atcggcgggc gtgagttcgc
    10921 aatgttgctc agcgctgaag agtgacggga acagtaccgc gagcggtttt tcttctcgct
    10981 gtttgcgttc gcgtaagcgt tggaccgcgc gcgagcttcg ggcatcgacg agtagctgaa
    11041 agccacccag gccccgaagg gccaagatct cgcctcgccg taagcactcg atcgcagccc
    11101 cgagggccaa gttcccgata acgggcaccg aatcggctcg cgtgcggtac tcgagttgcg
    11161 gaccacagtg agggcaagcg attgtctccg cgtgatagcg tcgttgcgtg gagtcccgat
    11221 actctcgttc gcaagctggg cagagcggga aagacgctaa agtcgtcttg gcgcggtcgt
    11281 aaggaactcc cagcgccacg ctgtagcgcg gaccacacgc cacgcagctg atgaaagggt
    11341 ggccgtaacg ccgatcttcg ggatcgacga gctcgcgcag gcagtccgcg cagaccatta
    11401 gatcgggcgg cagccccggt tgcgacgggc cgcccgcttc tgtcgtttgg cgacttctgg
    11461 taattgcgaa cccgccctcg gagcacggtt cggcctggat ttcccagtgc tccagactac
    11521 gaattcgagc cggggagggg atgcgcaaca agcgctggcg gaacatctcg agcaactcga
    11581 cgtccccctg agcttcgatg cgaacgccgc caccgctgtt gcacacgtag ccgcttagcc
    11641 ccaaagcttg ggcatgccgg gccgtatacg gacggaagcc cacgccctgt accgtgccac
    11701 aaacctcgat ggcccagcgc tttttcatga gatccgagcc ggcgtgtttt gaagccggaa
    11761 ttcgaggctg ccacaaaacg ccaacgccgc ccagccggag ctgggcggcg tcaggttcgg
    11821 tcgcgagccg cgcggagttt cgtgcgttcg cgagctcgag ccgatcaggt ctcgccgagg
    11881 tggtaaccgc gttcgccgtg gtcggtgaga tcgaggccgg tctcttcgtc ttccaccgac
    11941 gggcgaagcc cgagcactgc gcgcacggcg aatgcgagca cgcaagtcgc gacgacgctg
    12001 agcaccaccg tgaaggccat ggccttgagc tgctcgatcc agagcgtcga acccaccacg
    12061 gacgaaaggt tggtgttcag gttcgggttg acctcggcct tggcgaagaa acccgtgagc
    12121 agcgcaccca gcgtgccgcc gacgccgtgg acaccgaacg tgtcgagtgc atcgtcgtag
    12181 cccagcatcg ctttcagttt agtgcaggcg aagtagggaa tgaccccggc gagtacgccg
    12241 atgatgaccg ccgcgctggg cgtcacgaac ccggcgcccg gcgtgattac gaccaatccg
    12301 ccgacgatac ccgagcagaa gccgagaacc gtcggcttgc cgcgcgtcat ccactctact
    12361 gccggccagc tcgcgcccgc gacgccagcc gcaatcgtgg tcgtcacgaa tgcgttcgcg
    12421 gcgataccgt cggcaccgac ggcgctgcca gcgttgaatc cgtaccagcc gacccacaac
    12481 atgccggtgc caatcatggt cagcactagg ctgtgcggtg cgaagttctc gtggccgaac
    12541 cccttgcgct tcccgaggat gaccgccagc gtgatcgccg accagcccga gctcatgtgc
    12601 accaccgtgc cgcccgcgaa gtcgatcgcc ttgatggacg ccttggcgtt ccaaacgccg
    12661 ttcatcatgc catcgacgcc ccaaaccatg tgcgcgagag ggaagtagac gatgagcatc
    12721 cagccgagca gaaacagcat cagagagcca tatttcatgc gctctgcgat cgcgccgacg
    12781 atgagccccg gcgtgatgat cgcgaacatg agctggaaga tcgagaatac gttgtgagaa
    12841 acccaagcgc cgtagtcggt gttcggaatg gcgtcgacgc cattgaagaa taggaacttc
    12901 gttccaccga tcatgggccc tccgggcgcg aacgacaggc tatatccgaa cgcccaccac
    12961 agtaccgtca ccatgcccgc caagcccatg cactgggcca tcacactgag cacgttcttc
    13021 tgacgaacca agccgccgta gaagagcgcg agaccgggca gggtcatgaa tagaaccagc
    13081 gccgaacaca ccatcatcca gccgttgtgg ccggggccgg ggcccgagac cttcgaggtc
    13141 gtcgcatctg ctcgcgctcc gttgttcacg tacgcttcca ggtccagcag gcgcttttca
    13201 atctccgcag gggtcggacc ggcgctcgga gccggtgcag cctgcatcgc ggcaacaggg
    13261 gccgcggcag gtacggcggc ggcggtgggt gcggctgcgg tcgtcggggc gactgcgctc
    13321 gcagccgcac tgggcgctga gttggcttcc gcactgggtg cggcggcggc gctcgtggcc
    13381 gcgctggccg tggcaggggc attagccgac gcaactgcgg gggccggtga gggttcctgc
    13441 gcccatgccg gagaggccgt agccaagacc gttccgatta ataacaacca ccttttcaac
    13501 attccgagct ccttcaggat gtgcgccacc gcgcggcagc cctccctccg ggctgcgggt
    13561 caacgcgcag cggagttacc gctgggaatg tttctcacag ctttcgaaat ggtttcgttt
    13621 cgtgctttgc gtggccggat cagaaaagat gaaataaatt gaatggttgc attctgatac
    13681 gaggtttcgg ccaaagccca cctgaaacag tgctcgcgtt tgctatcgcc acggaactct
    13741 ggtttagtcc gggcgattcc gtgcaaaatc tggctaaaac tacgggtcgc actggcgttt
    13801 cgctctccgg tcgcttggta gctcgcgcaa gctggctagt ttgcggcgcg tcgatggcgc
    13861 taacgccagc ctgcaataag aaggatcctg cggcttgtga gcaggcccag cagaccatcc
    13921 ggcaggcgct ggatcgcgag cagttccagg cgataacccc ttggagggaa tacgcctggc
    13981 gccaatgcga ggatcgcacc gctttggcgg cgctcgatca gaaaatcgtg gctcgccaaa
    14041 atgaagtgca ggcgcggaac cgcgccgctg aagcacggcg ctccgagacc gctcagttgc
    14101 tgcgcgtctt cttgagctgg gtggccgaaa cccgcgccgc gccggatcgc gcgacggcaa
    14161 cgccggtctg cgatcctccg gcccccaatg atcccgagaa ggaaaaatcc aagcagcgca
    14221 tgtgtaccgc ctcgcggacg gtacaggggc cgagcggcat gcgacccttg tccgtgcgct
    14281 actttgcggc agagccgacc gccgcgcgtt tcatcatcaa cctgccggac gcaaccagct
    14341 gcgaggaatt gggcgcaatc aagtccaaga cctggcaggt tgccacgacc agcggcagca
    14401 cgaccgcgcg tttttcgtgt gattttacgt ctggaccact ctccggcatg cgagcggtgc
    14461 tgagccaagc ggtgaatgcc gacctgcacg tattcaatcc gacgtatctc gacaaggaac
    14521 cgcagtaccg cgcgatcttg gacagcccct gactgcgcgt cacttcgcgg ttcgaacccg
    14581 acgcgcgggg cctctcgtcc aatccatgtc aggcggacgt ccgcgcgcaa gaacacctac
    14641 ggtagggtga tggctcgcga acacctaccg tagggtgatg gcgcgagggg atcgctagct
    14701 aaagggtcgc ggggagatgg caaaaatcga cgctcccggg ggctgggaac gatttcagag
    14761 ctgaatcgcg gccatgcttt ggccgttcga agggtcactc cctttcgttg tctctgggcc
    14821 gcgcctcgcg atgaaaaccc cttctgaccg tccacctacc ttggacgcct tgccacgcgt
    14881 cagcctgcgc gcgcagctcg tgagcttgga aggccgcgcg gcaggtcgga agttcagaat
    14941 cgacggcagc gctaccatcg ggcgctcgct ggagagcacc g
//
LOCUS       DASTKU010000680        14698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1022579, whole genome shotgun sequence.
ACCESSION   DASTKU010000680 DASTKU010000000
VERSION     DASTKU010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14698
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022579"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFQ61_23640"
     CDS             <1..252
                     /locus_tag="VFQ61_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HET9957521.1"
                     /translation="VLARELRQLGIAVDLDARGGSLRSLLRRADALSASLCLVMGDSE
                     MDRGVVQIKDLAQHQQTELPRAAVSDHVRTWFARAEAAR"
     gene            288..2039
                     /locus_tag="VFQ61_23645"
     CDS             288..2039
                     /locus_tag="VFQ61_23645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957522.1"
                     /translation="MQLSKIGLGVSGLLLASPAHAQDPFGGGQGGGMFPGGGMGGPPR
                     SSGGKGKGKQPAKAPPPGTPEFHAASGASDQLVAPGSEPSLPANPLEKTKTVDATIGS
                     DHDPELEERGRSQKTTRRFYGPYYEEESGKYRLRLAFPVWGERTQPSRLNPSIPDRAS
                     VYGLYYNRRSNERADDVLFPLYWDMKNRVSGERTTIVGPLVHREAPDEHDNWLAPFYF
                     TGKRKHGGYTLIPPLLTYTRNDQDSGFTLVGPMFCSWKGGSSCDARTAQDIDLGVAPF
                     YFYGQNASSKYELIPPLLHYYSYDDRSLSWTNLWGPVYRHHSPKRDALHIFPFYYSLW
                     GQNERHTTLFPFFHYGHAGNESLLANPLFVLRKGEHGESTFVTWGYARYRGRTELDMI
                     TPLWWHFRDPDLSIDQKLLFPFFYSRQSPRESSLALFPFYGRFKRFGVSDSVWITPFF
                     QHSHDLRGFQTNIHPLVYIGREAANTHTVIAPFFWDFATPTSRTTVGFPLYWRFSDEK
                     EVSQVVGNVFYHEKKLSTGLDWEVHIFPAFSYGQTPDGHWWNVLFGLAGYTRRGAMTQ
                     MRTLWIPITLTGDPNKN"
     gene            2040..2765
                     /locus_tag="VFQ61_23650"
     CDS             2040..2765
                     /locus_tag="VFQ61_23650"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9957523.1"
                     /translation="MAEPLREPLLVPQPSPPVELVCFDLGGVLIRIAHHFDEGCAQAG
                     LVLRPLPKDHATVAARRRLTELLGTGQIQEHEWAERVSSLLKGLYSSEEILRIHHAFS
                     RGEYDGASALIREIHERGVTTACLSNTNHAHWRRLVHEEAPPSRVEPTRPLPPVPEFP
                     CVLALQHRHASHLFGVAKPDPRIYEMFESASAKRGPQILFFDDLIENVEAARARGWRA
                     ERIDPRAETVPQMRRLLAQYGVF"
     gene            2813..3874
                     /gene="argC"
                     /locus_tag="VFQ61_23655"
     CDS             2813..3874
                     /gene="argC"
                     /locus_tag="VFQ61_23655"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET9957524.1"
                     /translation="MKRYRASVLGGSGFAGAELIRRLLLHPEVELARVGAADHLGEPL
                     SDALPHLEGRTNLKFEALTPERAVADVDIVLMGLPQSASIPVVEAALARGVKVLDLSG
                     AFRLRKPEQYARYYGETHPRPDLLSGFVYGLPELNRAMIRGAQHVASPGCFATCIELG
                     LLPLAKAGLLPERIQTVGITGSSGSGVAPQLTTHHPTRANNLRTYRPLTHQHTPEIED
                     LLSQAAQHTVQLDFIPVSAPLVRGIFATSFARLTGAHTQDSLNAVFRQHYENEPFVRI
                     PARRAPEVVAVAGSNYAEVGFAMGASSPDSAATETVVSVFSCLDNLLKGGAGQAIQNM
                     NLMLGLPETLTLEDVGPYP"
     gene            complement(3891..4049)
                     /locus_tag="VFQ61_23660"
     CDS             complement(3891..4049)
                     /locus_tag="VFQ61_23660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957525.1"
                     /translation="MPGRVDNRDRYDFWIAAQSSLGLSAQFDAAFLGDEEGASGYSLG
                     LLASYAYH"
     gene            complement(4291..5223)
                     /locus_tag="VFQ61_23665"
     CDS             complement(4291..5223)
                     /locus_tag="VFQ61_23665"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9957526.1"
                     /translation="MSRKIPIAVLGLVLLSQAAACEREDASTAAYLSLAALNSPKSKA
                     KTPPAEVAARGERRAKAGGETDAEGTAKAEETAEAEETESPTLVAARGRRLAKNGACP
                     ADMVLVEGDYCPDVQLNCRKYMDPEGRYEQFRCAEYGPSTCKSKARKHMRYCIDRHEY
                     VPPGEALPANFKSFTHAERICKEQGRRVCMESEWNFACEGEDMRPYPYGFKRDAEACN
                     ADRMDILTDTGKLRDLRATPGSHPRCASPFGVQDLSGNLEEFVAIDRTNPVRPAMKGA
                     YWQPGRNFCRAAQTAHDRYYNGTETGFRCCAEAR"
     gene            complement(5465..6433)
                     /locus_tag="VFQ61_23670"
     CDS             complement(5465..6433)
                     /locus_tag="VFQ61_23670"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9957527.1"
                     /translation="MRVPASHRPFWRRARYGMGLALGVVLVRLALPDAGLPDDDNASI
                     DRQRVTARSDGSPSVESKVATPQNGRPSSPSTTSRPASVASPAATPAAPSPAAGEAIA
                     PNPEVCPGDMVLVEGLYCPDVVHTCLRWLDDEKLPFARCGEYLPKPQCRGEKLRLRYC
                     MDRREFTAEGEALPLNHQSFMKAEALCARVGKRVCTESEWNFACEGEEMRPYPYGFQR
                     RPVCNQDRSDLYEDNPRRQILRDNRRASGSMPECSSPFGVFDMVGNLDEPVLREAQRY
                     NYPFRNGLKGGWWMAGRNRCRPATTAHDDHYQDIQIGIRCCRDARR"
     gene            complement(6534..8450)
                     /locus_tag="VFQ61_23675"
     CDS             complement(6534..8450)
                     /locus_tag="VFQ61_23675"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9957528.1"
                     /translation="MSEPHCPSTPLKPLRHWTGWWLIMAIAALPACRAKPQASPGHAA
                     ASASALASSNAPMSTVVVDHGELDTKAPSDAPRLAATTIATTVYKLPDTASRKLGYIR
                     LGGIVRRDPASVSGKGCKGEFYRVYPMGYVCSEDATTDLESPIVRAASRRPDIDKPLP
                     YKYGFVRATSPQYLRIPSRAEQLKSEFKLEEHLSWFAEHRAEVQTVVPGANDVWLDAR
                     GFPRLSGKPPADFRKSTELSENELFGGATANDPIPFWLQGSRQIPNVAAYQVKEGALF
                     ADRVRRKTGLSFVAAFLTQDEGLERRFAVTVDMRLIPTTKVKPDTGSAFHGLELGDKV
                     ALPIAWVISDDAHAYQLIKDKDEVRGTEKLPRRAIVPLTGKARIKQGARYYQTVKDKT
                     VWLRASDLAVVDKPQAVPDVAERGEKWIDISITQQTLVLYEGKRPIYATLVSTGRDGL
                     GDPKTTLSTPRGTFRLQSKHIAAAMDSEENSSVAGGSRAPVATTNANASATIERLKRA
                     QAQGTKLDEEDRRRLANIEKGRDPEYGITVRRGSAGFELRDVPWIQYFASGYALHGAY
                     WHDVFGTPRSHGCVNLSPIDARVVFMFTDPPVPDGFHGINVGSDMGVGTLVSIREYRS
                     RARCKGGGGKGVAR"
     gene            8690..10165
                     /gene="miaB"
                     /locus_tag="VFQ61_23680"
     CDS             8690..10165
                     /gene="miaB"
                     /locus_tag="VFQ61_23680"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET9957529.1"
                     /translation="MPSYAVVTFGCQMNQHDSDRIGEVLRRSGYEEAADPERADLVLL
                     NTCSVREKAEQKLRSEVGRLGQLKRERPDLIIGVAGCLAQQEGNKLLSRMPQIDFLIG
                     PDHILDLPRALHELEQGGPPRAHVGFDLDEPEFLAARPEPGRVGVTAFVTVMKGCDER
                     CSFCIVPTTRGPERYRPARQIVDEIESLVALGVREVTLLGQTVNSYVDPEQALRAVPG
                     QTPDESQFAALIRAIAERVPTLARLRYTSPHPRHLTPALIAAHAELPLLPRHVHLPVQ
                     SGSDRVLKRMIRRYNRQEYCTRVTALRNVVPGLTLSTDVIVGFPGETEEDFEATLSLV
                     REIGFTGLFGFKYSPRPGTPALKLGDDIPEQEKSERLERLFRVSEAIRQEHLNSLVGA
                     ELEVLVEGRGKGDDFTGRSERNEIVHVSAGGLDPTGRVVRVRVEKAYKNSLAGVLLDR
                     LPRVPASVIPARPAAPLAARSELAPNDAVDPSPRRALPIVL"
     gene            10215..10763
                     /locus_tag="VFQ61_23685"
     CDS             10215..10763
                     /locus_tag="VFQ61_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET9957530.1"
                     /translation="MPALIAKFEESSPRFGSGVLLAENAVVIGDVELAEDVSVWFGAV
                     LRGDVGRIRVGARTNIQDLAMVHMTTALSNAEIAEDVTIGHAALVHGARIEPGVLVGM
                     GAILLDNAVIGAESIVGAGALVTSDFRAPPRSLILGRPAKVVRELSEEQYRQGRLSAL
                     HYVALARRHCASSGALQPPPTK"
     gene            complement(10742..10942)
                     /gene="thiS"
                     /locus_tag="VFQ61_23690"
     CDS             complement(10742..10942)
                     /gene="thiS"
                     /locus_tag="VFQ61_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HET9957531.1"
                     /translation="MHVIVNGDSLEAPEGLTVAGLIAHLGFSDGPVAVERNREVLPRA
                     EHAQTPLCENDVLEIVHFVGGG"
     gene            11050..12042
                     /locus_tag="VFQ61_23695"
     CDS             11050..12042
                     /locus_tag="VFQ61_23695"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9957532.1"
                     /translation="MKAIGARSSTREQLISEPSRRNRLGALVLLLCLPCSCCQRSVGA
                     SEASPVPAWGASGTLEVASWNVGWFGSGTQGPRDDVAQCNHVASVLRGSNIDVWALTE
                     VVDARAFAELVRELPEYDGLLASDLRVRDGSRFYDSGEQKPAVVFRRGYVKLSAAELI
                     LTEADSKFAGRPPLRVSLRVGEEPLELIVLHAKAYADAQSHARRTAASGALQGYLDEI
                     LPSAKVLVAGDFNDDIFESISRGRPSPYANFVGAGEKYRFLTAELSSRGLTSTVDHGA
                     MIDHHLASNELEALFIAGSTQVLSVDRYISNYAETTSDHYPVASRFKWPLPSSP"
     gene            complement(12220..12495)
                     /locus_tag="VFQ61_23700"
     CDS             complement(12220..12495)
                     /locus_tag="VFQ61_23700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957533.1"
                     /translation="MPTRTPRPLTAEALLTVLTGLAVAGCAKSAPEATPEKTNPPSVQ
                     PGAPASAPVIQAKEVPSSAPSASASGSAGSCAPNGCAPGACGGSKKK"
     gene            complement(12565..13998)
                     /locus_tag="VFQ61_23705"
     CDS             complement(12565..13998)
                     /locus_tag="VFQ61_23705"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET9957534.1"
                     /translation="MRKPLSALGLSIVLLGGCEPQQPAAAPLAAPPPAEAAPVAAAPA
                     AQPEAPKEAPKPVELTPEQSVKFYQECWSAFNAKDLTKYSACYSENASDEMVDANMPP
                     LVGKDITEKGAKVTLAAFPDLTGEAQLTLASGNRVAGVWLIKGTNQGPMHGPNGEIAP
                     TQKKIGYLMANVVELAGGKIARQFDYSDQRTFLTQLGLMPGQARKLMEQGAAEKPVVV
                     ATGSDAEKANLELYRKYSEAFNKHDATTLEGLLADDFVYSDQAMNADLVGKKEASRGV
                     KEMWKGLTDLRVDSREAFAAGDYVVSMSTLSGTNDGPLPAMKLWKKTGKKVNVNNLEV
                     MKVAGGKLKQHWVFSNGLAFAAQLGIGAPPVKEAKPAPAPVAAAPAKPGMNATPAAGA
                     APAAKPAPAAAAAGAKPAVGNPAAPPPAAAPAAAAAPAAPKPATPAAAAKPATPPAAP
                     KAAAPAAPPPAPKPAAPPAAPAPAPAK"
     gene            14184..14648
                     /locus_tag="VFQ61_23710"
     CDS             14184..14648
                     /locus_tag="VFQ61_23710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET9957535.1"
                     /translation="MKLSNKGRYAVRALFDIAFYNEGRPTQVKDIAERQAIPPRFLEQ
                     IFQDLKRSGIVGSKRGPQGGYSLGRRAAEIRLGDVVRALEGPIALGDREAGSRKAANQ
                     AGEARRVTEAVFKDLSTRVEACFDAVTIGDICTRAEEFGLKRPGAQRYVYVI"
BASE COUNT         2494 a         4681 c         4707 g         2816 t
ORIGIN      
        1 gtattggcgc gcgagctgag gcagctcggc attgccgtgg acctcgacgc gcgcggcgga
       61 tccttgagga gcttgttgcg acgggctgac gcgctctccg cgtccttgtg tttggtgatg
      121 ggtgactcag agatggatcg cggggtggtg cagatcaaag atctcgccca gcaccagcaa
      181 accgagctgc cacgtgcggc tgtatctgac cacgtgcgga cttggttcgc ccgcgccgag
      241 gcagcacgtt gagcgcgtcg cagcgtaggt tgtgggcgcg cccgtgggtg cagctatcga
      301 agatcggact gggcgtcagc gggctcctgt tggcgagtcc ggcgcacgct caggatccgt
      361 tcggcggtgg gcaaggcggc ggcatgttcc ccggcggggg aatgggcggc cctcctcgga
      421 gtagcggcgg caagggcaag ggcaagcagc ccgccaaagc ccctcctccg ggcactccag
      481 agttccacgc ggcctcgggg gccagtgacc agctcgttgc gcccggcagc gagccttcgc
      541 tgcccgccaa cccactcgag aagaccaaga cggtcgacgc aacgatcggc agcgatcacg
      601 atccggagct cgaggaacgt ggacgcagcc agaaaacgac ccgacgcttc tacgggccgt
      661 actacgaaga agagagcggc aagtatcgct tacggctcgc tttccccgtt tggggcgagc
      721 gcacgcagcc gtcgcgcttg aatccgtcga tcccggatcg cgcgtccgtg tacgggcttt
      781 actacaatcg tcgcagcaat gagcgggcag acgatgtcct gttcccgctc tattgggaca
      841 tgaagaaccg cgtcagcggg gagcgcacca cgatcgtggg cccgctcgtc caccgcgaag
      901 cgccggacga gcacgacaat tggctcgcgc ccttctactt caccggtaag cgcaagcacg
      961 gcgggtacac cttgatcccg ccgctcttga cctacacgcg caacgatcag gacagcggct
     1021 tcacgctcgt gggcccgatg ttctgttcgt ggaagggcgg cagcagctgc gacgcgcgca
     1081 cggctcaaga catcgatttg ggcgtcgcgc cgttctactt ctacgggcaa aacgcctcga
     1141 gcaagtacga gctgatccct ccgctgctcc actactactc gtacgacgat cgtagcttgt
     1201 cgtggaccaa cctatggggt ccggtctatc ggcaccactc gccgaagcgc gacgcactcc
     1261 acatttttcc gttctattac agcctatggg gccagaacga acggcacacg acgctgtttc
     1321 cgttcttcca ctacggccac gcggggaatg aatcgttgct cgccaacccg ctgttcgtcc
     1381 tgcgcaaagg tgaacacggt gagagcacgt tcgtgacgtg gggctacgcg cgttatcgcg
     1441 gtcgcaccga actcgacatg atcacgccgc tctggtggca cttccgagat ccggacttga
     1501 gcatcgatca gaagctgctc tttccgttct tctactcgcg ccagagccca cgcgagtcga
     1561 gcctagccct gttcccgttc tacgggcgtt tcaagcgctt cggcgtgagt gacagcgtct
     1621 ggattacgcc gttcttccag cacagccacg atctgcgtgg cttccagacc aatattcatc
     1681 ccctggtcta cattggacgc gaggcggcca atacccacac ggtgattgcg ccgttcttct
     1741 gggatttcgc gacgcccacc agccgcacga cggttggctt ccccttgtat tggcgcttct
     1801 ccgacgagaa ggaagtctcc caagtcgtcg gcaacgtctt ctatcacgag aagaagctca
     1861 gcacggggct cgactgggaa gtgcacatct tcccggcctt ctcctacggg cagactccgg
     1921 acggtcactg gtggaatgtg ttgttcgggc tcgccggcta cacgcggcgt ggcgcgatga
     1981 cgcagatgcg cacgctgtgg atcccgatca cgctgaccgg agatccgaac aagaactaat
     2041 tggctgagcc cctccgggag ccgctgctcg tgcctcaacc atcgccgccc gtcgagctcg
     2101 tttgcttcga tctgggcggc gtgttgatcc gcatcgccca tcatttcgac gagggctgtg
     2161 cgcaagcggg tctggtgctg cggccgctcc cgaaagatca cgccacggtt gccgcgcgcc
     2221 gacgcctgac cgagctgctg ggcaccggac aaatccaaga gcacgaatgg gcggagcgcg
     2281 tctcgagctt gctcaaaggt ttgtactcgt cggaagagat cctccgaatt catcacgcat
     2341 tctcgcgtgg cgagtacgac ggcgcatccg cgctcattcg agagattcat gagcgtggcg
     2401 taaccaccgc ctgcctttcc aatacgaacc acgcgcattg gcggcggctc gtgcacgagg
     2461 aggcgccgcc gtcacgagtc gagcccacgc gacccctgcc tccggtgcca gagttcccgt
     2521 gtgtgcttgc cctccaacac cgtcatgcct cccacctatt cggcgtggcc aagcccgacc
     2581 cccgcattta cgagatgttc gagagcgcga gcgcgaaacg cgggccgcag attctgttct
     2641 tcgacgatct gatcgaaaat gtggaggccg cgcgagcccg cggttggcgg gccgagcgga
     2701 ttgatccaag ggcggagaca gtgccacaaa tgcggcgatt gctcgcgcag tacggcgtgt
     2761 tttagcgagc cctgagttcg cgcggatggg tcacggcggg taccctaacg gaatgaagcg
     2821 atatcgtgcg tccgtcctgg gtggctccgg tttcgcaggg gccgagttga tacgccgtct
     2881 tttactgcat ccggaagttg agcttgcgcg cgtgggcgcc gccgatcacc tcggggagcc
     2941 gctctcggat gcgctgccac acctggaggg gcgcacgaac ctgaagttcg aggcccttac
     3001 gcccgagcga gccgtggctg acgtcgatat cgtgttgatg gggttgccgc agagcgcgag
     3061 cattcccgtc gtcgaggccg cgctcgcgcg tggggtcaag gtgctcgatt tatcgggggc
     3121 ctttcgtctg cgcaaacccg agcagtacgc gcgttattac ggtgagactc acccccggcc
     3181 cgatctgttg agcggcttcg tatacggatt gcccgagctc aaccgagcga tgattcgagg
     3241 tgcgcagcac gtcgcgtcgc cgggttgctt cgccacttgc atcgaactcg ggttgctgcc
     3301 gctcgcgaag gcggggttgc tgccggagcg cattcagacg gtcgggatca cgggctcgag
     3361 cgggagcggg gtggctcctc agttgacgac ccatcacccg acccgcgcca acaacttgcg
     3421 gacctaccgt cccctcaccc accaacacac gccggagatc gaggatctgc tgagccaggc
     3481 cgcacagcac accgtccaac tcgatttcat tccagtctcc gcgccgctcg ttcgtggcat
     3541 cttcgcgacc agcttcgcgc gactgacggg tgcccacacg caagacagcc taaacgcagt
     3601 gttccggcag cactacgaga acgagccgtt cgtgcgcatt cccgcacggc gcgcgcccga
     3661 agtggtggcc gttgcgggtt cgaattacgc ggaggtgggc ttcgcgatgg gcgcgagcag
     3721 tccggacagt gccgcgaccg agaccgtcgt cagcgtcttc tcgtgtctcg acaacctgct
     3781 caagggcggc gcgggccaag ccatccagaa catgaacctc atgctcggcc tgcccgagac
     3841 actgacccta gaagacgtgg gaccctatcc ctgatgagac gagcgttttc tcagtggtac
     3901 gcataggacg cgagcagtcc gaggctgtac ccagaggcac cttcttcgtc gcctaggaat
     3961 gcggcatcga actgcgcact caagcccagg ctgctctggg ccgcgatcca gaaatcgtat
     4021 cgatcacggt tatcgactcg gccgggcacg ccagatcgtg ggtgcgatcc aacgcgcgcg
     4081 cgaactccgc gggttgacgc tgggtcgcgc acgcggcgcc gatcaatccc aatacagcgg
     4141 ccccaagccg tagccacaag cagtttgcgt tggcggtgcc tgggtctacg ttcgtccttg
     4201 gtgcgctttt tccgagctga cccacgccca tggcgcgcgc tttacgcccc ccggtcacgc
     4261 tccgtcaaga accccgagcg aggcggagta tcagcgagct tccgcacaac accggaaacc
     4321 cgtctcggtg ccgttgtaat agcggtcgtg tgcggtctgg gcggcgcgac agaagttgcg
     4381 tcccggttgc cagtaggcgc ctttcatggc ggggcgcacc gggttggtac ggtcgatcgc
     4441 cacgaactcc tccaggttac ccgacaaatc ctgaactccg aatgggctcg cacaacgcgg
     4501 gtgagagccg ggcgtcgcgc gaagatcacg cagcttaccc gtgtccgtca ggatatccat
     4561 gcgatcggcg ttacaggctt ctgcatcgcg cttgaagccg tacggatacg gccgcatgtc
     4621 ttcgccttcg cacgcgaagt tccactcgct ctccatgcat acgcgccggc cctgctcttt
     4681 gcagatgcgc tccgcgtgcg taaagctctt gaaattggcg ggtagagcct cacccggagg
     4741 cacgtattcg tggcggtcga tgcaataccg catgtgcttc cgtgctttgg atttgcacgt
     4801 cgagggcccg tactccgcac atcggaattg ctcgtaacgg ccttcgggat ccatgtattt
     4861 gcggcagttg agctgcacgt cggggcaata gtcgccctct acgagcacca tgtccgcggg
     4921 gcacgcgccg ttcttcgcca agcggcgtcc gcgcgcggcc accagcgtcg gcgattccgt
     4981 ctcctctgcc tccgctgttt cctccgcctt agcggtgcct tccgcatcag tctccccgcc
     5041 agctttcgcc cggcgctcac cccgggccgc tacctcagcg ggcggcgttt tcgccttcga
     5101 tttgggcgaa ttcaatgcgg ccagactcag gtacgcagcc gtgctggcgt cttcacgctc
     5161 gcaggcagct gcttgagaga gcaacacgag tccgagcacc gcaatcggga tcttgcgcga
     5221 caaggcctct ttttttcggg atctctgggg ttgcgtcaag cgaaatctcg gaagggtcga
     5281 atcgccggag atcgcggaag ccagcgtttt cttgcgaaaa tcgccagagt caccgcaaca
     5341 atgcagcgag aggagagaga gactgagggt tttagtcctc agtgttcaga gccgaattca
     5401 agcaagatcc cgaaatcacg atattccggc tcgcgagtct gaatgcctat cttcgactcg
     5461 cgatctaacg acgagcgtcg cgacaacagc gaatcccgat ctgaatatcc tgataatggt
     5521 catcgtgagc ggtggtcgcc ggacggcagc ggtttcgtcc cgccatccac cagcccccct
     5581 tcaggccatt tcggaacggg tagttgtagc gctgagcttc gcggagcacc ggttcgtcca
     5641 agttgccaac catgtcgaac acgccaaaag ggctactgca ttccggcatt gaaccagacg
     5701 cacgccgatt gtccctcaag atttgccgac gggggttgtc ttcgtacaag tcgctgcggt
     5761 cttggttgca aacgggccga cgctggaagc cataagggta aggccgcatc tcctcgccct
     5821 cgcatgcgaa gttccactcg ctctccgtgc agacacgttt gccgactcgg gcgcacagcg
     5881 cctcggcttt catgaagctc tggtggttca agggcagagc ttcaccctcg gcagtgaatt
     5941 cgcgtcggtc catgcaatag cgcaggcgca gcttttcccc tcgacattgc ggtttcggga
     6001 ggtactcgcc gcagcgtgca aacggtagct tctcgtcatc gagccaacgt aagcaggtgt
     6061 gcactacgtc cgggcagtac agtccttcga ccaacaccat gtcacccggg catacttccg
     6121 gattgggcgc gatcgcctca ccggcggctg gcgatggtgc cgccggggtc gctgcaggtg
     6181 aagcgaccga ggctggtcgc gacgtggtcg atggagaact gggacgccca ttctgcgggg
     6241 tcgcgacctt gctctcgacc gacgggctac cgtcggatcg cgccgtcacg cgctgccggt
     6301 cgatactggc gttgtcgtca tccggcagtc cggcgtcagg cagagctagt ctcaccaaca
     6361 ccaccccgag cgcgagaccc atgccgtagc gcgcgcggcg ccagaacggg cgatgggagg
     6421 ctgggacacg catcgagtgc cagccactaa agttacacgc gaccgaggac ccacgcgaag
     6481 tttacggggt acttctcgaa ttgggccgcg ccggatgcga tccgcttgca cgcttaacgc
     6541 gcgacgccct tacctcctcc ccctttacac cgggcgcgag agcgatactc ccgaatcgag
     6601 accagcgtgc cgactcccat gtccgagccg acattgatgc cgtgaaaccc gtccggaacc
     6661 ggcggatccg taaacatgaa caccacccgc gcatcgatgg gtgagaggtt cacacagccg
     6721 tggctgcgcg gtgtgccgaa cacgtcgtgc caataggccc cgtgcaaagc gtaaccggac
     6781 gcgaagtatt ggatccacgg gacgtcgcgc agctcgaagc cggccgagcc gcgccgcacg
     6841 gtgatgccgt actcgggatc gcggcccttt tcgatgttgg ccaggcgacg gcggtcttcc
     6901 tcgtcgagct tcgtcccctg cgcttgggcg cgcttcaagc gttcaatcgt ggcgctggcg
     6961 ttggcgttgg tcgtggcaac cggcgctcgg ctgccgcccg ccacgctcga gttttcttct
     7021 gaatccatgg ccgcggcgat gtgcttgctc tgcaagcgga acgtaccgcg tggggtggag
     7081 agcgtggtct tggggtcacc caagccatcc cgaccggtgg aaaccagggt ggcgtagatg
     7141 ggtcgcttgc cttcgtacaa caccaaggtt tgctgagtga tcgagatatc gatccacttt
     7201 tcgccccgtt ccgcgacgtc gggcacggct tgcggcttgt cgacgacggc caagtccgag
     7261 gcgcgcagcc aaaccgtctt gtctttgacg gtttggtagt agcgagcgcc ttgcttgatg
     7321 cgcgccttgc cggtcaaagg cacgatggcc cttcgcggta acttctcggt gcctcgaacc
     7381 tcgtccttgt ccttgatcag ctggtaggcg tgggcatcgt cgctgattac ccacgcgatc
     7441 ggcagggcga ctttgtcgcc cagctccagg ccgtgaaacg cggagcccgt gtctggtttc
     7501 accttggtcg tggggatcaa gcgcatgtcg accgtgaccg caaaacgtcg ctcgagcccc
     7561 tcgtcttgcg tcaggaaggc ggctacgaaa ctcaggcccg tcttgcgccg cacccgatcc
     7621 gcgaacagcg cgccctcttt gacctggtag gcggccacgt tggggatctg ccgcgaacct
     7681 tgcagccaaa acgggattgg atcgttggcc gtcgcgccgc cgaacagctc gttttcactg
     7741 agttcggtgg acttgcggaa gtccgccgga ggtttgcccg agaggcgtgg gaatcctcga
     7801 gcgtcgagcc atacgtcgtt ggcgccggga actacggtct gaacttctgc gcgatgttcg
     7861 gcgaaccagc tcaagtgctc ctccagcttg aactcgctct tgagctgttc ggcccggctg
     7921 ggaatcctca ggtactgcgg cgaggtggcg cgcacgaagc cgtacttgta cggcagcggc
     7981 ttgtcgatat ctggtcggcg gctcgccgcg cgcacgattg gcgattccaa atcggtggtc
     8041 gcgtcctcgc tgcagacata gcccatcgga tagacgcggt agaactcgcc cttgcagccc
     8101 tttccggata ccgaagcggg gtcccgcctc acgatcccgc cgagtcggat atagccgagc
     8161 ttgcgagagg ccgtgtcggg caacttgtat acggtggtcg cgatcgtggt cgcggcgagc
     8221 ctcggggcgt cgctcggcgc tttggtgtcg agctcgccgt ggtctacgac gacagtcgac
     8281 atgggtgcgt tactcgaggc gagcgcgctg gccgaggccg cggcgtgacc cggcgacgct
     8341 tgcggcttgg cacggcacgc cggcagggcc gcgatcgcca tgattagcca ccagcccgtc
     8401 caatggcgca gaggtttcag gggggtgctt gggcaatgag gctcggacac cggcgttgcg
     8461 ctacatatcg cggcgcggtt gcggggcaag tgggggccgt tcgggaagcg ccccctggga
     8521 gcagattggc ccgcccgctt gcgcgcttgg gcgacctcct gcatataccg ccgcctgtag
     8581 gctgccggtt cggaaccggc caagcccgtg tcccggtcgg caaactcagc cccgttcgcc
     8641 cgcccagccc cgttcaccag ccccgttcaa cagcccagcc ccgtttatca tgcccagcta
     8701 cgccgtcgtt acctttggct gccaaatgaa ccaacacgat tcggatcgaa tcggagaggt
     8761 tttgcgacgt tcgggctacg aagaggcggc cgatcccgaa cgagcggatt tggtgctgct
     8821 caatacttgt tcggttcgtg aaaaggccga gcagaagctg cgcagcgaag tggggcggtt
     8881 ggggcaactc aagcgagaac ggccggatct gatcatcggg gtggcaggct gtttggccca
     8941 gcaagagggc aataagctgc tcagccgaat gccccagatc gattttctga tcggccccga
     9001 tcacattctc gatttgccgc gggctttgca cgagctggaa caaggcgggc cgccgcgagc
     9061 acacgtgggc ttcgatctcg atgagcccga gtttctcgcc gcacgaccgg aaccgggccg
     9121 cgtgggagtg accgccttcg tcacggtgat gaagggctgc gacgagcgtt gctcgttttg
     9181 catcgtgccg accacgcgag gtcccgagcg ttaccgacca gcgcgtcaaa tcgtggatga
     9241 aatcgagtcg ctcgtggccc tcggggtgcg cgaggtcaca ttgctcggac agacggtcaa
     9301 tagctacgta gatccggagc aggccttgcg tgccgtgccc gggcagacgc ccgacgagag
     9361 tcagtttgca gccttgattc gcgcgattgc cgagcgggtg cccacgctcg cgcgactccg
     9421 ttacacgagc ccccaccctc ggcacctcac gcccgccttg atcgcggccc atgcggagct
     9481 tccgttgttg ccccggcacg tccacctccc cgtgcagtcg ggtagtgatc gggtgctgaa
     9541 gcgcatgatt cgtcggtaca accggcagga gtactgcacg cgggttacgg ccttgcgcaa
     9601 cgtggtgccg ggcttgaccc tctccaccga tgtgatcgtc gggttccccg gggaaaccga
     9661 ggaagacttc gaggctacgc tgagcttggt tcgcgaaatc ggcttcacgg gactattcgg
     9721 gttcaagtac tctccccgcc ccggcacccc cgctctcaag ctcggggacg atatccccga
     9781 gcaggagaag agcgagcgcc tcgagcggct atttcgggtc tccgaggcca ttcggcaaga
     9841 acacttgaac tctctcgtgg gcgccgagct cgaggtgctg gtcgaggggc gcggcaaggg
     9901 ggacgacttt accgggcgca gcgaacgcaa cgagatcgtg cacgtgagcg ctggtggctt
     9961 ggatccgacg ggtcgcgtcg tccgcgttcg agtcgagaag gcctacaaga actcgctggc
    10021 gggcgtgctg ttggatcgac tgcccagggt cccagccagc gtaattcctg ctcgccccgc
    10081 cgccccactt gccgcacgct cggagctggc gccgaacgac gctgtggatc cgtctccacg
    10141 gcgcgccttg ccaatcgtct tgtgaaacgg ctgtcgccaa aacggatttc ctagtttcgg
    10201 aggtcgcgcc tgccatgccc gcactgatcg cgaagttcga agagagctcc ccccgcttcg
    10261 ggagcggcgt actgctcgcg gaaaacgccg tagtgattgg ggacgtggag ctcgcggagg
    10321 acgtgagtgt ttggttcggc gccgtactgc ggggagacgt tggtcgtatt cgagtgggcg
    10381 cgcgcacgaa cattcaggac ctagccatgg tccacatgac gaccgccctt tcgaacgcgg
    10441 aaattgcgga agacgttacg attgggcacg ccgcactcgt acacggcgca cgcatcgaac
    10501 ccggcgtgtt ggtcggcatg ggcgcaatct tgctcgacaa cgcggtgatc ggagctgaat
    10561 cgatcgtagg cgcgggcgct ctggttacct ccgattttcg ggctccaccg cgcagcctga
    10621 tcttgggtcg tcccgcgaaa gtggtgcgcg agctcagcga ggagcagtac cggcagggtc
    10681 gcctctccgc gttgcactac gtggcgctcg ctcgtcgaca ctgcgcgagc agcggcgcgc
    10741 ttcagcctcc ccccacgaag tgaacgatct cgagtacatc gttttcgcag agcggagttt
    10801 gagcgtgctc ggcgcgcggc aaaacttcgc ggttgcgctc gaccgcgacg ggcccgtcac
    10861 tgaagccgag gtgtgcgatc aggcccgcga cggtgaggcc ctccggggct tccagcgaat
    10921 caccattgac gatcacgtgc atggcccgag attttgcacg cgccgcggtt ttttcaagcg
    10981 gccgagcgat tcgagagccc agcccacatt ctacgcaact gcccgcgcag tttggccgat
    11041 atgcaggtca tgaaagcgat aggcgcgcgg tcttcgactc gagaacagct gatttccgaa
    11101 ccgtcgcgcc ggaatcggct cggggcgctc gtgttgctcc tgtgcctgcc ttgctcgtgc
    11161 tgtcagcgct cagttggcgc ttcggaagca agcccagtcc cggcttgggg ggcgagtgga
    11221 acgctggagg ttgccagctg gaatgtggga tggtttggga gtggtaccca gggcccgcgc
    11281 gatgacgtcg cccagtgcaa tcacgtcgcg agcgtactgc gaggcagcaa catagacgtc
    11341 tgggcgttga ccgaagtcgt cgatgctcgc gcgttcgccg agttggttcg cgagcttccc
    11401 gagtacgacg gactactcgc ctcggatctg cgcgtccgcg acggatcgcg attctacgat
    11461 tcgggcgagc agaagcccgc agtggtgttc cgccgcggct acgtgaagct gagcgcggcc
    11521 gagttgatcc tgaccgaagc ggattcgaag tttgcgggcc gtcccccgtt gcgcgtgtct
    11581 ttgcgcgtgg gcgaggagcc tctggaattg attgtacttc acgcgaaggc ttacgcggac
    11641 gcgcagagcc atgcgcgtcg cactgccgct tccggcgcac tccagggcta tctggacgaa
    11701 attctgccga gcgcgaaggt gctcgtggcg ggcgacttca acgacgatat cttcgagtcc
    11761 atcagccgcg ggcgaccatc gccgtatgcg aacttcgtgg gtgcgggcga gaaataccgc
    11821 tttctcaccg cagagctctc gtcacgaggc ctcacctcca cggtggatca cggcgcgatg
    11881 attgaccacc acctcgcgag caatgagctc gaggcgctct tcattgccgg atcgacgcag
    11941 gtgctctccg tcgatcgcta catttcgaac tacgcggaaa ccacgagcga ccattacccg
    12001 gtcgcgagcc gcttcaagtg gccgctccct tctagccctt agaacctatc ccaatcggag
    12061 ctagtcgccg acggccgata gttcggtcac cgcctccggc gctcctagtg ggattagtgg
    12121 gattagcggg ataggttctt agctcaaggc gcgatcgctc tggagtcgcc acgtgtcacg
    12181 cagcggagtc catatggcgc gagcgcagca cggccgtgtt cacttcttct tcgaaccgcc
    12241 gcaggcgccc ggtgcgcagc cgttcggcgc gcagctcccg gcgcttccgc tggccgatgc
    12301 tgagggcgcg ctcgacggca cttctttggc ctgaatcacg ggggctgaag cgggcgcgcc
    12361 cggttgcacg ctcggaggat tcgtcttctc cggcgtcgct tcaggggcag acttcgcgca
    12421 acccgccacc gcgaggcccg tcagtacggt caaaagagcc tcggcggtga gcggtcgtgg
    12481 agttcgagtc ggcattgagc cccgagagtt gcgcggcggc gcggccttcg caagcctcga
    12541 ggcaagcgaa cgcccgtcga ggaactactt cgccggagcg ggtgccggag ctgcgggcgg
    12601 agccgcaggt tttggcgccg gaggcggggc ggccggggcc gctgccttcg gtgccgcggg
    12661 cggcgttgca ggctttgccg ccgcggcagg cgtcgcgggc ttcggggccg ccggagccgc
    12721 cgctgcagct ggcgccgctg cagggggcgg ggccgccgga ttaccgactg ccggtttcgc
    12781 gccagccgca gccgccgccg gtgcgggctt agcagcgggc gccgcccccg ctgcgggagt
    12841 cgcgttcatg cccggcttgg ctggggccgc tgcgaccggc gcaggtgcgg gcttggcttc
    12901 cttgaccggc ggagcgccaa tgccgagctg cgccgcgaag gccaggccgt tggagaacac
    12961 ccagtgctgc ttgagcttgc ctccggcaac cttcatgacc tcgaggttgt tgacgttgac
    13021 cttcttgccg gtctttttcc agagcttcat cgccggcagt gggccgtcgt tggtgccgga
    13081 gagcgtgctc atcgacacca cgtagtcacc cgccgcgaac gcttcgcggc tgtcgacgcg
    13141 caggtcagtc agacctttcc acatctcctt cacgccgcgg gaagcttcct tcttaccgac
    13201 caggtcggcg ttcatcgctt gatccgagta gacgaagtcg tccgcgagca ggccctcgag
    13261 cgtcgtcgcg tcgtgtttgt tgaaggcctc gctgtacttg cgataaagct cgaggtttgc
    13321 cttctccgca tcactgccgg tggcgaccac gacgggcttt tcggcagccc cttgctccat
    13381 cagtttgcgc gcctgccccg gcatgaggcc gagttgggtc aggaaggtgc gttgatcgga
    13441 gtagtcgaat tggcgtgcga tcttcccgcc cgcgagctcg accacgttgg ccatcaaata
    13501 cccaatcttc ttttgggtgg gcgcgatctc tccgttgggc ccgtgcatcg gcccttggtt
    13561 ggtgcccttg atgagccaca ctccagcgac acggttgccg ctggccaacg tgagttgggc
    13621 ttcgccagtc agatccggaa acgcggccaa ggtcactttg gcgcccttct cggtaatgtc
    13681 cttgccgacc agaggcggca tgttggcgtc caccatctcg tcgctggcat tctccgagta
    13741 gcacgcgctg tacttggtca ggtccttggc gttgaatgcg ctccaacact cctgatagaa
    13801 cttcaccgat tgctcgggcg tgagctctac gggcttcggt gcctccttgg gcgcttccgg
    13861 ttgagcggca ggcgcggccg caaccggcgc cgcctcggca ggcggcggtg ccgccaacgg
    13921 agcggcggcg ggttgctgcg gttcgcagcc gccaagtaac acgatgctga gaccaagagc
    13981 gctgagcggt ttcctcatgt tcgttcccct ttccgaaccc cgcttctaac ccgagctggc
    14041 cctgttcttc cagcatcaac actggttgta tgtttcggcc gtccaccggg catgcggtgt
    14101 aacggcgcag atgtaggccc tagtgttttt ccccgttcgc gggttcttga caaacccgat
    14161 gtaggaatgc aaaccaaggc accatgaagc tatcgaacaa gggccgctac gccgtgcgag
    14221 cgctgttcga tatcgcgttt tacaacgaag gccggccgac tcaggtgaaa gacatcgcgg
    14281 agcggcaggc tatcccgccg cgcttcctcg agcagatttt ccaggacctg aagcgatcgg
    14341 gcatcgtcgg ttcgaaacgt gggcctcagg gtggctacag cctagggcgg cgcgcagccg
    14401 aaattcgact cggggacgtg gtgcgagcgc tcgaagggcc gattgccctc ggggatcggg
    14461 aggcgggcag ccgcaaagcc gccaatcaag ccggagaagc gcggcgcgta acggaagccg
    14521 tgttcaaaga cctttcgacg cgcgtagagg cttgcttcga cgcggtcacc atcggagaca
    14581 tttgcacccg cgccgaggag ttcgggctca agcggcccgg agcacaacgt tatgtctacg
    14641 tcatctgaat cgaactctgc ccccgcgctg aagtctcatc cgctggtgtt ctccagtg
//
LOCUS       DASTKU010000681         3202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1246925, whole genome shotgun sequence.
ACCESSION   DASTKU010000681 DASTKU010000000
VERSION     DASTKU010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246925"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..1321
                     /locus_tag="VFQ61_23715"
     CDS             284..1321
                     /locus_tag="VFQ61_23715"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9957536.1"
                     /translation="MSPPLLNRETPFVIVFNAASGSGDARRAQAEMTEVLNAGHQEHE
                     FFVVEDPKQLQDLARRAAEKAVQRNGAVIVAGGDGTINAVAQATLKTGRPFGIVPQGT
                     FNYTTRARAIPLDTALATHALLNARLSAMQVGLVNEKVFLVNASLGLYPQLLEERETY
                     KSQYGRTRGVALWAGLRTLLRDHRQLTLEIEHDTERELVRTTSLFVGNNPLQLEQAGL
                     PEAQAVQRRRLAAVIVKPRKTRSLLWLAARGALGQLGGAANVRNFAFKRLVVSPARGN
                     YMRPFKVATDGEICFMQPPLVFRVASESLWLMTPAAESESTVTPVASGSGTADPAGMS
                     GRDDRTKEPAA"
     gene            1338..2180
                     /locus_tag="VFQ61_23720"
     CDS             1338..2180
                     /locus_tag="VFQ61_23720"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9957537.1"
                     /translation="MAVLVQISDPHFGTERPHVVEGLVALVREQQPALVVLSGDITQR
                     ARRAQFAAARAFLDRLNAPHVLVIPGNHDIPLFNPVARWLYAYRNYREAFGPELEPEY
                     ESDDLLVLGLNTTRPYRHKDGEVSDAQIRRVSDRLSAARARQLKIVVTHQPVRVIRDS
                     DVSNLLHGHERAVRAWASAGADLVLGGHIHLPYVRQLSDCFPDLPRRLWAVQAGTAVS
                     HRVRDGVTNSVNIIRYAPRHAGQPDHSDLRAAGTVCDVERWDYHVGQERFEREHVQTI
                     ALER"
     gene            complement(2186..>3202)
                     /locus_tag="VFQ61_23725"
     CDS             complement(2186..>3202)
                     /locus_tag="VFQ61_23725"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HET9957538.1"
                     /translation="IKSVQQYSMLRDMREFLQWRRGDAAILAEANVLPEVNVHYFGDD
                     GDRMHMMFNFQVNQNLFYALASGDTRPLVKALESTRERPESAQWAMFLRNHDELDLGR
                     LTETQRQRVFAEFGPEQEMQLYDRGIRRRLAPMLHGDRRRLEVAYSLLFSLPGTPVIR
                     YGDELGMGDDLSLPERNCARTPMQWSTEPNAGFTRARKPVLPVISEGPYGYHKVNAAE
                     QRRDTQSMLNWTERMIRMRKEVPEFGWGDFSVVDTGQRCVLGLRYDWRNNSVLTLHNF
                     GDQAVEVSLKLERGRGGKLVNLLTGDHSDADARGRHRIQMEPYGYHWYRMGGLGYLLE
                     RTNF"
BASE COUNT          600 a         1011 c          990 g          601 t
ORIGIN      
        1 ttagtgtctt gttgtaccgg cccttcgaat cgtgagtagc gtagcggttt tgacgcgcga
       61 ggcgcctttg cacccgaaag ctcgcgggcg gcgacgccgg gggacgttcg cgcctaggag
      121 gagcccctcc tctgaggccc accaaaactc gacgtggacc atggtgcgaa taacggcggt
      181 acggtggttg catcttcgca ggctaaccga ttgattcggt gaagcttcgg agcttgctgg
      241 cacgctccgc cgaccacggc ctgcgttgaa cccaaaggat tacatgtcgc cgcctctgct
      301 caatcgagag acgcccttcg tcatcgtgtt caacgccgcc tcgggttcgg gagacgcccg
      361 tcgggcccaa gcggaaatga ccgaggtcct gaacgcgggc caccaagagc acgagttctt
      421 cgtggtcgaa gatccgaagc agctgcaaga tctcgcccgc cgagcagcag agaaggccgt
      481 ccagcgcaat ggcgcggtga tcgtcgcggg tggcgatggc accatcaacg cggtagcgca
      541 agccaccctg aagaccgggc ggccctttgg catcgttccg caaggcacct tcaactacac
      601 cactcgcgcg cgagcgatcc cgttggacac cgccctggcc acgcacgccc tgttgaatgc
      661 gcggctctcg gccatgcaag tggggctggt caacgagaag gtgttcttgg tcaatgctag
      721 cttggggctc tatccccaat tgctcgaaga gcgcgagacc tacaaatcgc agtacggccg
      781 cacacgtgga gtcgcgctat gggcaggatt gagaacgctg ctgcgggacc atcgtcaact
      841 caccctcgag attgagcacg ataccgagcg agagctggtg cggaccacct cgctcttcgt
      901 cggcaataac ccgttgcaac tcgagcaggc agggctaccg gaggctcaag ccgtgcaacg
      961 acgccgattg gccgcggtga tcgtgaagcc acgcaagact cgctcgctgt tgtggctggc
     1021 ggcgcgcggg gccttggggc agctcggcgg cgcggccaat gtcaggaact tcgcgttcaa
     1081 gcggctggta gttagcccgg cccgaggcaa ttacatgcgg ccattcaagg tcgcaaccga
     1141 cggcgagatt tgcttcatgc agccgcccct tgtattccga gtggcctccg agtcactctg
     1201 gctgatgacg cccgcagcag agtccgaatc tacggtcacg ccggtcgcga gcggcagcgg
     1261 gacggccgat ccagcaggga tgagcggtag agacgaccgc accaaagaac ccgcggcctg
     1321 acgcaccgac gaaatcgatg gcggtcctgg ttcagatatc ggatcctcac tttggcaccg
     1381 agcgaccgca tgtcgtcgaa ggcttggtgg cgctggttcg cgagcaacag cccgcgttgg
     1441 tggtgttgtc cggggatatt acgcagcgtg ctcgacgcgc tcagttcgcg gcggctcgcg
     1501 cgttcttgga ccggcttaac gcgccccacg tgctagtgat tccgggcaat cacgatattc
     1561 ccttgttcaa tccagtagcg cgctggctgt acgcatatcg gaattaccgt gaagcgttcg
     1621 gcccggagct cgagcccgag tacgagtccg atgatttact cgttttgggc ctgaatacga
     1681 ctcgcccgta ccgccacaag gatggcgaag tttcggatgc tcagattcga cgagtgagcg
     1741 accggctgag cgccgcgcgg gctcgacaac tcaagattgt ggttacccac cagcccgtgc
     1801 gcgtgatccg cgactccgat gtcagtaact tgctccacgg ccacgaacgc gccgtacgcg
     1861 cgtgggcgag cgcgggcgcc gacctcgtgc tgggcgggca catccacctg ccgtacgtgc
     1921 ggcaactctc ggactgcttc ccggatctgc cgcgcaggct gtgggccgtt caagcgggga
     1981 ccgccgtatc ccatcgcgtg cgcgacgggg tcacgaactc cgtgaacatc attcgatacg
     2041 ccccccgcca cgccggccaa cccgaccact cggacctgcg cgccgcgggc acggtctgcg
     2101 atgtagagcg ttgggactat cacgtgggcc aagagcgctt cgagcgcgaa cacgtacaga
     2161 cgatcgcgct cgagcgctaa ccgccttaga agttggtccg ttccagcaga taaccgaggc
     2221 cacccatgcg ataccaatgg tagccgtagg gctccatttg aatgcgatgg cgcccccgtg
     2281 catcggcatc gctgtgatcg cccgtgagca ggttcaccag cttgcctccc cgcccccgct
     2341 cgagcttgag ggatacctcc accgcttggt ctccgaagtt gtgcaaagtg agcaccgagt
     2401 tattacgcca gtcgtagcgc aggcccaata cgcacctttg tcccgtgtcc acgacgctga
     2461 agtctcccca gccgaactcg ggaacttcct tgcgcatccg gatcatgcgc tccgtccagt
     2521 tcaacataga ctgagtgtct cgccgctgtt cggcggcatt gaccttgtgg tacccgtagg
     2581 gcccctccga gatcacgggc aacacgggtt tacgcgcgcg cgtgaagccg gcgttgggct
     2641 cggtcgacca ctgcatgggc gtgcgagcac aattgcgctc gggcaagctc aaatcgtctc
     2701 ccatgccgag ctcgtcccca tagcgaatta ctggggtacc cggcaggctg aacaataggc
     2761 tataggccac ctccagacgg cgtcgatcgc cgtgcagcat gggggccaag cgccggcgaa
     2821 ttccgcggtc atagagctgc atttcctgct cggggccgaa ttctgcgaat acgcgctgac
     2881 gttgggtctc ggtcaatcta ccgaggtcca gctcgtcgtg gtttctcaga aacatggccc
     2941 attgggcgga ctccggtctc tctcgggtcg attcgagagc cttgaccaac gggcgcgtgt
     3001 ccccggacgc gagagcgtaa aataggtttt ggttcacttg gaagttgaac atcatgtgca
     3061 tgcgatcgcc gtcatcaccg aagtagtgca cgttgacctc cggtaagaca ttggcctcgg
     3121 ccaggatcgc ggcgtcaccg cgacgccatt gcaggaactc acgcatgtcg cgcaacatcg
     3181 aatactgttg cacgctcttg at
//
LOCUS       DASTKU010000682        13041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1022682, whole genome shotgun sequence.
ACCESSION   DASTKU010000682 DASTKU010000000
VERSION     DASTKU010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13041
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022682"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1575
                     /locus_tag="VFQ61_23730"
     CDS             346..1575
                     /locus_tag="VFQ61_23730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957539.1"
                     /translation="MTEMQPDYEGRVIAGRYRVERLLGQGGMGSVWAGRHLTLNQLVA
                     IKFIHPRLTSSEDALRRFEVEAKAAARLRSLHAARVYDHGVTADSQPYIVMEYLEGET
                     LGQSVRRRGHLPLLELVRVISETARALGAAHEQGILHRDLKPDNIFLATERDAPPFGY
                     VVKVLDFGIAKIAEDDGLGTGTQAGMVLGTPHYMSPEALTASAPVGAAADIWSLGACA
                     YAAACGTMPFYGDAIGDVVLKVCVAPMPVPSKVQPALPKAFDEWFARACARDVSQRFA
                     SMKEAEQAILELDAWNQRRREQSLYELGKIAGATELDSLHPPSSRRALLLGGGLAGAS
                     VMLALLGNYAYQRTRAAADEAARVAASARAVMDRENERRLREAEQRFWSQQPDAGPPS
                     EDAGVPNQNATQRKKKR"
     gene            1958..2515
                     /gene="frr"
                     /locus_tag="VFQ61_23735"
     CDS             1958..2515
                     /gene="frr"
                     /locus_tag="VFQ61_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET9957540.1"
                     /translation="MIEDVLAELKQATERAKEALRRELAKLRTGRAHAGMLDSLRVEY
                     YGSITPVAQMATVNVPEPRMITVKAWDKSQTSAIEKAIRESDLGLNPQVDGDLIRIPI
                     PPLSEERRKDLVKVARKQGEESKVVIRKARHEALDMLNEIKTEGHASEDDVERGKKKV
                     EEWISESTASVDQIIQQKEKEILTI"
     gene            3284..4165
                     /locus_tag="VFQ61_23740"
     CDS             3284..4165
                     /locus_tag="VFQ61_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET9957541.1"
                     /translation="MRAVAAGITDVGLQRDHNEDSFAILKDHALYIVADGMGGHRAGD
                     VASRLATEAIVDFFRTTASDDFTWPFHFDARLSEEENRLLTGIKIANRQIIDRSTRSR
                     EYHGMGTTVVGALFNAKRGKMYIGHVGDSRAYRVRSGQIVQMTRDHSLVNDYLMAMPE
                     LTEEQRSELPKNVITRALGMQDQVSVDLQSDDAQAGDVYVLCSDGLSGMIDDQEIVSV
                     VASTPDLEEVCRRLVALANEHGGEDNITALVVRIDPENEAQKVSGDTMPIIPTHRSTD
                     VTAETPPAGVAAVKPGG"
     gene            complement(4411..4986)
                     /locus_tag="VFQ61_23745"
     CDS             complement(4411..4986)
                     /locus_tag="VFQ61_23745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957542.1"
                     /translation="MHRCYACALPLILGLAALSCSDPVPASAAVGLTLKMQPSASCPY
                     DSNLVDDIGQIGGDGKPQSPNSLAGTPGKRVYSGESGLDASCSITGGGPFSISGGGSM
                     SDPRISFNIEGTMNANGQGTATMTFASNSIGEGVTSDPGACTLTAVQTSDGVQIHPGA
                     MWAQFNCPSVKNVPTINCMAFGELILENCKK"
     gene            complement(5659..5735)
                     /locus_tag="VFQ61_23750"
     tRNA            complement(5659..5735)
                     /locus_tag="VFQ61_23750"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5699..5701),aa:His,seq:gtg)
     gene            complement(5861..6931)
                     /locus_tag="VFQ61_23755"
     CDS             complement(5861..6931)
                     /locus_tag="VFQ61_23755"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET9957543.1"
                     /translation="MNSTLAPSPNQKPLLFDDPVFDEHHARGDHPECPERLQAARRAV
                     ERTRPQLEFTQVPARDATEEELARVHTPEYLQQLDQLAGQWSFIDADTYVCPASIGAA
                     KRAAGATVQLVEGLLSGMAPWGLALVRPPGHHACANRAMGFCLLNNVAVAAAHARALG
                     AERVLVLDIDVHHGNGTQEIFYRDPSVLYVSLHQFPYYPGTGAATELGEGRGRGYTVN
                     VPLSGGAGDTTYRTAFERIVLPIIEEYHPSLTLVSAGFDAHERDPLGGMMLSDAGYSS
                     LIQRLKSSLPARHPIGLVLEGGYDFIGLQTALEHTLLSMASPRVTPSLQALQTPASPG
                     LGSHQRDLERAEAVQRVHWYLG"
     gene            7315..8769
                     /locus_tag="VFQ61_23760"
     CDS             7315..8769
                     /locus_tag="VFQ61_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HET9957544.1"
                     /translation="MRWKIIVVNAGILIVVGLLSFVLLATSLRELFANPAERKREVEQ
                     ALRAASAQLSLDGLRMERWLEQQSDNESARAVFSIGVPEARNQAATVDANRLRDAAVS
                     EPTFAKMAPTLVLYVDDQGIAIGRNGSALMRGDNIAAVYPDIRKSLKAGSTGSAVWLN
                     RQRQEQLLVSYAPLRSETGKILGALVVGTPLNDERMTRTSELTSGHTLVFQVVNDKST
                     ELLADSNDASSEVVNATSSSAVVSAARAAQTSGTVVHAEAEISDHVFGAIPLFGYAEQ
                     KGVLVAAVPSSIVGSVYAVLWPLVAVAALGLVLVVIGGTLLGNYISGPVSELEDGLLA
                     IINGSSDLRFQIEHDELGGLVFRINSLLNALMGVPEDTTDEQGRPSHAPRAQDFQEAL
                     AVDESAVSEQGDPDVAEALAKEPADKYYRRLFEEYIAAKRKLGDPVDHITFEAFRVKI
                     QASEQEMSQKHGRPVRYQVQLRGNSVVLTAISVS"
     gene            8978..9721
                     /locus_tag="VFQ61_23765"
     CDS             8978..9721
                     /locus_tag="VFQ61_23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9957545.1"
                     /translation="MGLFDIFSKKKGDAPRVSERELLRLERLVGNKLSQNIDRQEAIE
                     TLCAMGNARSAAALLRRFDWMLDPSITDQEEKDACLRGIVAAGEDALSPVRAYCQKAE
                     SLTWPIRVLKSIVKHEALQEELLALLDQYDTEYVRNAEPKVQLITELAQHKTDDVRVA
                     LEPFLQDASEPVRFATVTSLFEIGDPASVPAFIAALTDEESQRIKNRIAQRLAELGMA
                     IPTDQATACAKALPTGFSLENGRVRALTA"
     gene            complement(9908..10786)
                     /locus_tag="VFQ61_23770"
     CDS             complement(9908..10786)
                     /locus_tag="VFQ61_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957546.1"
                     /translation="MNTSKGKTSKRLLGVLAAALSLFAFTERAEAQEILLTGPLAGAP
                     AVRKLRLYREGRFEFAPTVSFTLLDEYQRTILLGARLGYNFTDWLGIGVWGAYGGVLQ
                     MPTALTEHIQDVNTQRRTSAGDGYERSLTGLLTDTNLGTDFKKQVALIDWAVSPQITV
                     VPLRGKIALFQSIYIDTDLYVFGGPAFVGLKERKDCERGTCGGSFERVSRTAIAPTFG
                     LGLSFYANSWGGLGLEYRALPFSWNTGGFDTAGSGKDEKFPDERISSADRQFKFNQLL
                     SVSYTIFLPTQFRISE"
     gene            complement(10804..11760)
                     /locus_tag="VFQ61_23775"
     CDS             complement(10804..11760)
                     /locus_tag="VFQ61_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HET9957547.1"
                     /translation="MKKMRVGLLVAAALGILPANGWAQGKGAKAAEPEAPSGDAEAAE
                     GQTPTEGEATPAEGGDATPAEEGQGGGLEEICKIDPAACPNLDMEKEAARPLRENIYA
                     MQQIYSLRVRRFELNPYWGFTLNDQFVSHPGPGLAINYYITNVLAVGVNGNYYRWFNA
                     DQEFNAQVRRSARVAVPLTEYDWGANLNFTYVPAYGKFAGLGDWIFQYDAYVVAGVGV
                     LSTRPIPVIDPDNRNFTFDTKLAFNAGIGLRVNLNRWFAITGEIRDYIFPDKLENLAV
                     DETDAGNKSKWYESKSRLTNAVQAQLGVSIFLPFSFDYRLPK"
     gene            complement(11974..>13041)
                     /gene="ftsY"
                     /locus_tag="VFQ61_23780"
     CDS             complement(11974..>13041)
                     /gene="ftsY"
                     /locus_tag="VFQ61_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET9957548.1"
                     /translation="GVEPSAPPPPPSRHRETPIAPRASEAPRADDAKKEPRHSAPPAP
                     SSLERLRKGLSRSRSSEGFFGRLRALFRGKTEVSPELAREIEEVLLTSDVGAETTKAL
                     LERVQQELANGEHTDPEKVWDAIALEAKRLLDFGAVGPIEVRHHPTVVLMVGVNGAGK
                     TTTIGKLATKLQAAGHKCVLAAGDTFRAAAVQQLVVWGDRVGCEVVRGKEGSDPGSVL
                     FEAIKRAQDTGADVVLADTAGRLHTKSNLMAEMKKIAKTAEKALPGAPHETFLVIDAT
                     NGQNALAQAREFREALPLSGIVLTKLDGTAKGGVVLGIAHTLKLPVRFIGLGERPDDL
                     HEFRPAEFVDAMLGRESDNRA"
BASE COUNT         2496 a         3954 c         4164 g         2427 t
ORIGIN      
        1 atcatggaag ccggccctcg gcatgccctc cgccacctct tggagcagcg ctacgtcagg
       61 ttcctgttcg gacagaaccc cgatcgttcg ctcgatgtcg tagcgccgat cgacgccacc
      121 aatgcctttg tgaatgttcc agctcacgac gcgcaggcgc atcgaaaatg cagtatggca
      181 caggtcggcg cgtgcccagg gccgaactcg agctcgccgt aaatcgcaat ttcacgcacc
      241 taaagccacc cacgtggaac aacgagacat cggggcaagg agactcgaac actgagccgc
      301 ccagcagtgc tatagagacc caggcgtgtt gctgggttcc tcaccatgac cgagatgcag
      361 cccgactacg agggtcgcgt catcgcgggt cggtaccggg tcgagcggct gctcggtcag
      421 gggggcatgg gttcggtctg ggcgggtcga catctaacgc tgaatcaact cgttgcgatc
      481 aagttcattc acccccggct cacgagcagc gaggatgcgc tccgccgttt cgaggtggag
      541 gccaaagctg cggcgcgctt gcgttccctg cacgcggccc gtgtctacga ccacggtgtg
      601 acggcagaca gccagccgta catcgtgatg gagtacctcg aaggggagac cctggggcag
      661 agcgtgcgcc gccgaggaca cttgcccttg ctcgaactcg tgcgagtgat ctcggagacg
      721 gcgcgtgcgt tgggtgcagc acacgagcag ggcattttgc accgggattt gaagcccgat
      781 aacatctttc ttgcgacgga acgggacgct cctcccttcg gctacgtggt gaaggttctc
      841 gactttggca ttgccaagat cgccgaagac gatgggctag gaaccgggac gcaagcgggg
      901 atggtgctcg gaactccgca ctacatgagt cccgaggccc tcacggcttc cgcaccggtg
      961 ggtgccgcgg cagacatctg gtcgctcgga gcttgcgcgt acgctgcggc gtgcgggacc
     1021 atgccgttct acggcgacgc gatcggggac gtggtgctga aggtttgcgt cgcgccgatg
     1081 ccggtgcctt ccaaggtcca gccggcgctg cccaaagcat tcgacgaatg gttcgcgaga
     1141 gcttgcgcac gtgatgtcag tcagcgcttc gcttcgatga aggaggcgga gcaagcgatt
     1201 ctagagctgg acgcatggaa tcagagacga agggagcaga gcctctacga gctgggcaaa
     1261 atcgccgggg caacggagct cgacagcctt catcctccgt cgtctcgacg cgcgttgctg
     1321 cttgggggtg ggctagccgg cgcgagcgtg atgctcgccc tgctcggcaa ctacgcctat
     1381 cagcgcacgc gggctgctgc ggacgaggct gcgcgcgtag cggcgagcgc gcgcgcggtc
     1441 atggaccgcg agaacgaacg tcgtctgcgt gaagcggagc agcgtttctg gtcccagcag
     1501 cccgatgcgg gcccccccag cgaggatgca ggcgtcccga atcagaacgc cacccagcgc
     1561 aagaagaagc gctgactttg cctagggctc caaatgaatg ggccgggatg cacgtcgctt
     1621 tcgcggtccg gcacgcaccc taagacaggc tcgtggagac aggcacgggt cggggcgcac
     1681 acccgagaag cacgccggag atccacggtc agacgggtag gttcccaccg gctcgggagc
     1741 tgcggtgcga aggcaatgga actgcgctgc ttctcgggct ataccgacgg ccggcgcgtg
     1801 atttgcgcct cacacccagt gcctcttgtt ctggcaaggg tgtgccttcg gctggcaagc
     1861 tgccagcaac ctggctgagc ccgtgccggc gcctgagctg gcaccgagcg gagcccccgc
     1921 tctgacaagt tactctcgaa cagaataggt ctggctgatg atcgaagacg ttttggctga
     1981 gctgaagcaa gcaaccgaac gcgcgaaaga ggcgctccga cgcgagcttg cgaaactgcg
     2041 taccggccgt gcacacgctg gcatgctcga ctccttgcgc gtcgagtatt acgggtcgat
     2101 cacgccggtt gcacaaatgg cgacggtaaa cgtgccggag ccgcgcatga tcaccgtgaa
     2161 ggcctgggat aagtcgcaga cctccgcgat cgagaaggcg atccgggaaa gcgatctggg
     2221 cctgaacccg caggtcgacg gcgatctgat tcggatccca atcccgccgc tcagcgaaga
     2281 gcgtcgtaag gatctcgtca aggtcgcgcg taagcagggc gaagaatcga aggtcgtgat
     2341 tcggaaggcg cgccacgagg cgttggacat gctgaacgaa atcaagaccg aaggtcacgc
     2401 gagcgaagac gacgtcgagc gtggaaagaa gaaggtggag gagtggatct cggagtccac
     2461 ggcttcggtc gatcaaatca ttcagcagaa agagaaggaa attctcacca tttgaggggc
     2521 cgcgtggcgg cgtgttcctg ccgctgacag aagcaaaggg gcgttgctgt tcatcaatgc
     2581 ccctgacgcg ctcatccaac cacacctggc caaccgcgct atcgtgtcca tggacacaga
     2641 catgtccatg gacacagaca tgtccaggga cacagacatg tccatggaca cagacatgtc
     2701 cagggacaca gatgaggcgc cgagagtcag ctgcgccgtt gtcagcagcg agcctcagca
     2761 gcacgacccc tccgcggcac gagcgctggt aggtgtcccg acgcgacagc gggcgtgtgc
     2821 tcctgggcgc tccccacgtt ccgatttgct tgccggcggg gtcggcgtca cggcgtcggg
     2881 tggcctcgag cgctctggcc cgcgatgagt ctcggggcgc tctgccgtgg gcggcgtggc
     2941 aagttccgcc ctgcgagcca gagcatggaa gcagcccacg ggacacccgt cgatgaacgg
     3001 aattatgagc aaagggcatc ggacgagggc aaaactcgcg gatttccgct ctggttcgga
     3061 actacgagca tagaaggatc ttcgatccag gggttggtgt gaccgatggc cccactcgaa
     3121 ggccccgcac cgtgggccac tccgggccgc ccctggaact gtgaaccggc gggcagtccg
     3181 tatctttcag gtgaccttag gtattcggac tccttgacag cttgacccgc tcgtgaaact
     3241 tttttcgagg attgggcaat tggttctcgg aaggaatgag tcgatgcgag ccgtagcggc
     3301 cggaatcacc gacgtcggtc tgcaacgcga ccataacgag gacagctttg ccatcctcaa
     3361 ggaccacgcg ctgtacatcg tggcggatgg catgggcggt catcgtgcgg gcgacgtagc
     3421 cagccgtttg gcgaccgagg cgatcgttga tttcttcagg acgacggcgt ccgacgattt
     3481 cacttggccg tttcacttcg atgcgcgctt gtcggaagag gagaatcgtc tccttaccgg
     3541 catcaagatc gccaatcgtc agattatcga tcgcagcacg cgctctcgtg agtaccacgg
     3601 catgggtacg acggtggtcg gagcgctgtt caacgccaag cgcggcaaga tgtacatcgg
     3661 gcacgtgggg gacagccgcg cgtaccgcgt tcgctcgggg cagatcgtgc agatgacgcg
     3721 cgatcactcg ctcgtgaacg attacctgat ggcgatgccc gagctgaccg aagagcagcg
     3781 cagtgagctc cccaagaacg tgatcacccg ggcgctcggg atgcaggatc aggtctcggt
     3841 ggatttgcag agcgatgatg cccaggccgg tgacgtgtac gtgctctgct cggatggcct
     3901 gagcgggatg atcgacgatc aggagatcgt ttcggtcgtg gccagcaccc cggatttgga
     3961 agaggtgtgt cgtcggctgg tggcgctcgc caacgagcac ggtggtgagg acaacatcac
     4021 tgcgctggtc gtccgcatcg atccggaaaa cgaggcgcag aaggttagcg gggacacgat
     4081 gcccatcatt cccacgcatc gttccacgga cgtgaccgcc gagacgccgc cggcgggtgt
     4141 tgctgcggtg aagcccggcg gctgagtcgc gctcgcttta aaacgactcc gctagcgagc
     4201 gcggcgcctc tttgcggcgc cgcacctctc tcgcggcctt gcgccagacg gcgacgttcc
     4261 ctccttctgg tcactgcgag gtggttcgct ccgactgtcc cacggcatca cggtgaggcg
     4321 ggttccgcag tccgcgcgtt tgcgtccgtt tgcacaacga cggcgccacg cagcgccccc
     4381 ctctggcgct tccagcaggc gcgtcctcag ctacttcttg cagttctcta gaatgagctc
     4441 accgaaggcc atgcagttga tggtggggac gttcttcacg gaggggcagt tgaactgggc
     4501 ccacatggcg cccgggtgga tctgaactcc gtcggaggtt tgcacggccg tcagtgtgca
     4561 agcgcccgga tcgctggtga ccccttcacc gatgctgttg cttgcgaacg tcatcgtggc
     4621 cgtgccttgc ccgttcgcgt tcatggtgcc ctcgatgttg aacgagatgc gtgggtcgct
     4681 catcgagccg ccgcccgaga tcgagaatgg gccacccccg gtaatgctgc aggatgcatc
     4741 gaggccggac tcaccactgt agacgcgctt gccaggagtt cctgcgaggc tattcggcga
     4801 ttgcggctta ccgtctcccc cgatttggcc aatgtcgtcc acgaggttcg agtcataggg
     4861 acaagaagcg gacggctgca tcttcagcgt cagcccgact gcagccgatg ctgggacagg
     4921 gtcactacaa cttaatgctg ccagaccaag aatcaaaggc agggcgcagg cgtaacatcg
     4981 gtgcatcgtg aacctccaac tgcagtctac tccgagttct atagagatcg atccacgaga
     5041 aacgtgcgat ccgccgaaag ctgatgaacc gcgcgggacg ggtcgattcg gatgcactcg
     5101 gggtgagagg ttgccgctcg catggatact gatcgcacgc ttggaatgag caatgcccac
     5161 gctccgggaa tcagacgtta tccagccgtc tacggcgcgc tcgaggccga gctactctat
     5221 ccggctgcca tcctcggttg caggttatcg tttgcagctt atcagcggat aatgcccgcc
     5281 tctagaaccc agacaccacg gggatgcgtg ggattcggca gcgttcctca cgacggcctc
     5341 ctcgcctgaa actccagagc gctgagccat cctcggcaag cgtgcaccgg aaacgaacgc
     5401 gggagctcag tgttcccatc gggcgcttcc cgtccggaag tcacttccgg atctcgattc
     5461 gccttggcct aaggcaacgc gaggtcgagt aacaccgccc gaacgacttg gcctaggccg
     5521 gcggctccat gcgacgacct cgattgcctg cacagacaat cgtcagcaga gactgttggg
     5581 gagcggacca gcgtggcacc cgctcatgaa gctttgggtg acgagtgtct gcccaaggca
     5641 aagtcgggtg gctatttttg gggtgaccga cgggattcga acccgcgacc tcgagagcca
     5701 caatctcgcg ctctaaccaa ctgagctacg gccaccgtac aactgagatt tcaagccgct
     5761 gggactgtcc gtcccagcgg gacagggggt ttagcgcaca ggtgtgccca gatgcaaccc
     5821 ggaaatctgg tcgccggaaa gtagagccct ggccaacgta ctagcccagg taccagtgca
     5881 cccgctgaac ggcctccgct ctttcgagat cgcgctggtg actgcccaac ccagggctcg
     5941 ccggcgtttg tagcgcctgc aagctcgggg tgaccctcgg gctggccatg ctgagcagcg
     6001 tatgctcgag ggccgtctgc aggccgatga agtcataacc cccttccaac accaggccga
     6061 tgggatggcg cgcgggtagg ctcgatttga ggcgctggat cagcgaagaa tagcccgcat
     6121 cactgagcat catgcccccg agcggatctc gctcatgagc atcgaagccc gcgctcacca
     6181 gagtcaagct cgggtggtac tcctcgatga ttggtaacac gatgcgttcg aaggcggtgc
     6241 gataggtcgt gtcgcccgcc ccgcccgaaa ggggcacgtt gacggtgtag ccacgccctc
     6301 ttccttcgcc gagctcggtg gcagcccctg ttcccggata gtacgggaac tgatgcagcg
     6361 agacatacaa tacgctcggg tctcgataga agatctcctg tgttccgttg ccgtgatgga
     6421 cgtcgatgtc caggaccagc acgcgctctg ccccgagagc tcgcgcgtgc gcggcggcta
     6481 ctgcgacgtt gttgagcagg caaaacccca tcgcgcggtt cgcacaagcg tggtgcccgg
     6541 ggggccgcac gagagccaac ccccacggcg ccatgccgct cagtagacct tcgaccaatt
     6601 gaaccgtcgc gcccgcggcc cgcttcgcgg ccccgatgga ggccgggcac acgtatgtat
     6661 ccgcatcgat aaacgaccac tggccggcta actggtccag ttgctgcaga tactcagggg
     6721 tatggacgcg cgcgagctcc tcctcggttg cgtctcgcgc ggggacctgc gtgaactcga
     6781 gctgaggtcg cgttcgctcc acggcgcgac gcgcagcttg caatcgctcg gggcactctg
     6841 ggtgatcgcc gcgcgcgtgg tgctcgtcga acactggatc gtcgaacaat aggggcttct
     6901 gattggggga gggagcgagc gttgaattca tggtgcaggg gtcacgaacg acgctgtcga
     6961 aatcgggctc gaaattggga cagcccaccc atcttcgatg actgagctcg cctgaatgag
     7021 gcgcgagctc tcgcaaactc agaggctcga ttgcaggagt agtttcccct gcgcgacact
     7081 cccacagtag caaaacataa ggcttcgggc cggccggaac tccactttcc cacacgctca
     7141 cgcggcttgc caaagcgaca cgctccgccg cgccgatcca tcaagaacct agagctcctg
     7201 gtcgtgaagt agtaccgtgc tcggtgccga cttacgcgcg acgccgtggc gttgcttacc
     7261 ggttagaacg aggtggccga ctgccgctgg gcgagcggtc accaaggggt ccagatgcgc
     7321 tggaagatca tcgtcgtaaa cgcaggtatc ctgattgtcg tcggtttgct gagcttcgta
     7381 ttgctcgcga cgtctttgcg agagctgttt gccaatcctg ccgagcgcaa gcgcgaggtc
     7441 gagcaggcat tgagggcggc gagcgcgcag ctctcgctgg acggtttgcg catggagcgc
     7501 tggctcgagc aacaatcgga caacgaatcc gcgcgcgccg tgttcagtat tggcgtcccg
     7561 gaagcccgaa accaggcggc tacggtggac gccaatcgct tacgggacgc cgcggtgtcg
     7621 gagcccacct ttgcgaagat ggcgccgacc ttggtgttgt acgtggacga ccaaggaatc
     7681 gcgattgggc gcaatggctc agcgctcatg cgcggagaca acatcgcggc cgtttacccg
     7741 gacatccgca agtcgctgaa agccggcagt acgggtagcg ccgtgtggct gaatcgtcag
     7801 cgtcaagagc agctgctcgt gagctatgcg ccgctgcgct ccgaaacggg gaagatcctg
     7861 ggcgcgttgg tggtgggcac accgctcaac gacgagcgca tgacgcgcac gagcgagctc
     7921 accagcggtc acacgctggt attccaggtc gtgaacgaca agtcgacgga gctcttggcc
     7981 gacagcaacg atgcgagctc cgaagtggtg aacgcgacga gctcttcggc cgtggtttcg
     8041 gcggcgcgcg cggcgcaaac gtctggcacg gtggtgcacg ccgaggcgga gatttcggat
     8101 cacgtgttcg gcgcgattcc gctgttcggc tacgccgagc agaagggcgt gctggtcgcg
     8161 gccgtcccga gctcgatcgt gggcagcgtg tacgccgtgc tttggccgct cgtcgcggtg
     8221 gcggctctcg gtctggtatt ggtggtgatt ggtggcactc tgctcggcaa ctacatttct
     8281 ggcccggttt cagagctaga ggacgggttg ctggccatca tcaacggcag ctccgatctg
     8341 cgtttccaga ttgagcacga tgagctcgga gggctggtat tccggatcaa ttcgctgctc
     8401 aacgccttga tgggcgtgcc ggaagacacg accgacgagc aggggcggcc gtcccatgcg
     8461 ccgcgcgctc aagacttcca agaggctttg gcggtggacg agagtgccgt gtcggagcag
     8521 ggcgatccgg acgtcgcgga ggcgctcgcc aaagaacccg cggacaagta ctaccggcgg
     8581 ctgttcgaag agtacatcgc ggccaaacgc aagctcggcg acccggtgga tcacatcacc
     8641 ttcgaggcgt ttcgcgtgaa gatacaagcc agcgagcagg agatgtccca gaagcacggg
     8701 cggcctgtgc ggtatcaggt ccagctgcgc ggaaattccg tagtgttgac cgcgatttcg
     8761 gtgagttgac acgctaatcg tccccggggg gcactggcgc gtgggcagac gtgccgcctg
     8821 cccacggccg cgcaatcgat cccgaggcga gccctcaatc gcagggctcc ggcgatcggc
     8881 cgtgtacgtt ccaggcgggc gcgctatatc accgcacgca ggacgccacg tctgcgggaa
     8941 cggcccgcgc ggccatccgc cacaagttgg aagaatcatg ggtttgttcg atattttctc
     9001 gaagaagaag ggggacgccc cgcgagtttc cgagcgcgag ctgttgcgct tggagcgcct
     9061 ggtcggcaac aagctatcgc agaatatcga tcgccaagag gccatcgaga cgctgtgtgc
     9121 gatgggcaac gcccgcagtg cggcggcgct acttcgccgc ttcgattgga tgctggatcc
     9181 ttccatcacc gatcaggagg agaaggatgc gtgcctccgc gggatcgtcg cggccggtga
     9241 agacgcgctc tctccggtgc gcgcctactg ccaaaaggcg gagagcctga cttggccgat
     9301 tcgggtgctg aagagcatcg tcaaacacga agcattgcag gaagagctgc ttgctctgtt
     9361 ggatcagtac gacacggagt acgtgcgcaa cgcggagccc aaggtgcagt tgattaccga
     9421 gctcgctcag cacaagaccg acgacgtacg cgtggcccta gagccgttct tgcaggacgc
     9481 gagcgagccg gtgcgcttcg cgaccgtaac cagcttgttc gagattggcg atcccgcgag
     9541 cgtgcccgcg ttcattgcgg cgctgacgga cgaggagtct caacgcatca agaatcgtat
     9601 cgcacaacgt ctggcggaac ttggaatggc aatccccact gaccaggcga cggcgtgtgc
     9661 caaagcgctc cccaccgggt tcagtctcga gaacggccga gtgcgcgcgt tgacggcttg
     9721 accgagaccg cgcgcgtgag cgagcccata cgagggcaca aagcaaaacg gagcagacga
     9781 ttcgcctgct ccgtttttgc tttacctctg cttggtcgcg cgaggcttac gccttacccg
     9841 agcgccgctt ccgtgatacc ccgagccggc ccgcgcgcga ggcgggccaa cacggggcta
     9901 cccgcggcta ctcgctgatg cggaactggg tgggcaggaa gatcgtgtag ctcacgctca
     9961 acaattggtt gaacttgaac tgacgatctg cgctgctgat cctctcgtcc gggaacttct
    10021 cgtccttgcc cgagcctgcg gtgtcgaagc cgccggtgtt ccaagagaag gggagggcgc
    10081 gatactcgag ccccaagccg ccccagctat tcgcgtagaa gctcaagccg agaccgaacg
    10141 ttggtgcgat tgcggtgcgc gagacgcgct cgaaactccc gccgcaagtt ccgcgctcac
    10201 agtctttgcg ttccttcaaa ccgacgaacg cgggtccacc gaacacgtac agatccgtgt
    10261 cgatgtagat gctctggaag agagcaatct tgccgcgcaa cggaacgacg gtgatttgcg
    10321 gggacaccgc ccaatcgatc agcgcgacct gcttcttgaa gtcggtgccg aggttggtgt
    10381 cggtgagcaa gccggtgagg gatcgctcgt agccatcacc ggcgctcgtt cggcgctggg
    10441 tgtttacgtc ctggatgtgt tcggtgagcg ccgttggcat ctgcaagacg ccaccgtatg
    10501 caccccaaac accaattccg agccagtccg tgaagttgta accgagccgc gcacccaaca
    10561 gaatcgtacg ttggtactca tccaaaaggg tgaacgacac cgtaggagcg aactcgaagc
    10621 gcccttcgcg gtacaagcgc agcttgcgca ccgcgggggc gccggcgagc ggccccgtga
    10681 gcaggatctc ttgtgcctct gcgcgttccg tgaatgcgaa gagcgagagg gcggccgcta
    10741 ggactcccag gagccgcttc gacgtcttgc ctttgctagt gttcatgcct cgcccttcgc
    10801 tcatcacttc ggcagcctgt aatcaaacga gaacgggagg aagatcgaaa ccccgagctg
    10861 cgcctgcacg gcgttcgtca gacgggattt gctttcgtac cacttcgact tgttgccggc
    10921 gtccgtctcg tcgaccgcga ggttttcgag cttgtccggg aagatataat ctcggatctc
    10981 gccggtgatt gcgaaccaac gattgaggtt gacccgcaga ccaatgccgg cgttgaacgc
    11041 gagcttcgta tcaaaggtga agttgcggtt gtcggggtcg atgaccggga tgggtcgcgt
    11101 cgacaacaca cccacgcccg cgacgacgta ggcgtcgtac tggaagatcc agtcgcccaa
    11161 gccggcgaac ttgccgtacg cgggtacgta cgtgaagttc aggttcgcgc cccaatcgta
    11221 ctcggtgagc ggcaccgcga cgcgcgcaga gcgacggacc tgagcgttga actcctgatc
    11281 cgcattgaac caacggtagt agttaccgtt cacaccgacc gcgaggacgt tggtgatgta
    11341 gtagttgatc gcgagacccg gacccgggtg cgagacgaac tggtcgttca gcgtgaagcc
    11401 ccagtacggg ttcaactcga agcgacgcac tcgcagcgag taaatctgct gcatcgcgta
    11461 gatattctcg cgaagcggcc tcgcagcttc tttctccatg tccaggttcg gacatgcagc
    11521 agggtcaatc ttacagatct cttcgagacc tccaccttga ccttcttcag caggagttgc
    11581 gtcaccaccc tctgcgggtg ttgcttctcc ttcggttggt gtttggcctt ctgcggcttc
    11641 tgcatctcca ctcggagcct ctggttcagc cgccttggca ccctttccct gcgcccagcc
    11701 gttggcaggg aggatgccga gggccgctgc cacgaggagc ccgacgcgca ttttcttcat
    11761 cgatgcctct ttcggtttcc ccttcaacga ctgcttctct cgatccgcaa atgctgagaa
    11821 tgattaacta attccgccgt ccaaaaacgc cggggcacta tatcacgcta aaaatcagcc
    11881 gatcaacaag caaagaaatt cagtcaaaac ttaatctgga cgcgatcttg attagctcgc
    11941 tgtacttcgg tttgaagttg ggttttgcga gcttcacgcg cgattgtcgc tttcacgtcc
    12001 cagcatggcg tccacgaact ccgcagggcg gaactcatgc aaatcatctg ggcgctcacc
    12061 taaacctatg aagcgcacgg gcagcttcag cgtgtgagcg ataccgagca cgacaccgcc
    12121 tttcgcggtg ccgtcgagtt tcgtcaatac gatgccagac aaaggtagtg cttcgcggaa
    12181 ctcccgcgct tgagcaagtg cgttctgccc attagtggca tcgatcacta agaacgtctc
    12241 atgcggcgca ccgggtagtg ctttttccgc tgtcttggcg atcttcttca tctccgccat
    12301 caagttagac ttcgtatgaa ggcgccctgc cgtatcagcc aggaccacgt cggccccggt
    12361 gtcctgagca cgcttgatgg cttcgaatag aacgctgcct ggatcggagc cttctttccc
    12421 tcgcacgact tcgcagccga cacgatcgcc ccaaacgacc aactgctgga cggctgcggc
    12481 tcggaaagtg tcgccagcag cgagtacaca cttgtgacct gcggcctgca acttggtggc
    12541 gagcttcccg atcgtagtcg tttttccggc gccgttgacg ccaaccatca atacgacggt
    12601 gggatggtga cgaacctcaa tcggccccac tgcgccgaag tcgaggagcc gcttggcttc
    12661 gagagcgatc gcgtcccaaa ccttctccgg atccgtgtgc tcgccgttcg cgagctcttg
    12721 ctgcacgcgc tcgaggagag ccttggtggt ctcggcacca acgtcggagg tgagcagaac
    12781 ctcttcgatc tccctcgcca gctctgggct gacctcggtc ttgccacgga acaacgcgcg
    12841 gagccggccg aagaaacctt cgctactgcg cgaacgcgac aaacccttac gcagccgctc
    12901 gagtgagcta ggtgccggcg gcgcggagtg acggggttct tttttggcgt cgtcagcgcg
    12961 gggggcttcg gacgcccgag gcgcgatcgg cgtctcgcgg tgccgagatg gagggggtgg
    13021 gggcgcgctg ggctcgacac c
//
LOCUS       DASTKU010000683         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1693535, whole genome shotgun sequence.
ACCESSION   DASTKU010000683 DASTKU010000000
VERSION     DASTKU010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693535"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1621)
                     /locus_tag="VFQ61_23785"
     CDS             complement(113..1621)
                     /locus_tag="VFQ61_23785"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HET9957549.1"
                     /translation="MFAKRLVRVSLKTVVAACLTSGVLGLATDAAAQEAKKQSDTSNE
                     SRALRIALEEVGLLLAGTGYYFMQHEVNSLDWTFDYSWDTVEAKLTGDAYSFDTNFFK
                     TNTLAHSGAGTLYYLAARGNYLSPAESLAVGFFASAAWEFFAEFRERVSINDQMVTPF
                     GGFAFGEAMTQVGRLFLRGCDSTSFGILGAVFAPSESLHRSIDGTQLPRARRCNSSGF
                     STRDDYVLRLGLGASFSGTWSARDGRGWLLAHGEALILDVPKNQTVQEPWRAFYDGGL
                     SQLSVRVQAAADELREIDVIARGSLFGSAYRDVTTNTVALLALGAGVNYSERRYSAAP
                     APRDPFFVLEAPALFAYWRRGYGHQRLELGLVAGASFVQAGAFALPVYLQTNTADELT
                     PVVANRGYSHGFGLTLSPHLRWTSPFHEVWLGARVDRAWAVRSRIGDQLASSPVDEEE
                     ARRRLRAALSLGEDERWRFRTEADFIVRRGTLGELERRRGEFTWTTGVEGRF"
     gene            complement(1638..>2086)
                     /locus_tag="VFQ61_23790"
     CDS             complement(1638..>2086)
                     /locus_tag="VFQ61_23790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957550.1"
                     /translation="YTTSGGLWGGIAGALIAGAAESQTSYRAERAYAIGALGYNAALL
                     AALATSPQLAPSVARVRLVDLGGLAGGLLGAGGYLLIADDAASSRAAMGAAAGGALLG
                     LGFSWWATSGMPEEPHPLAEQPLLSRLRPTVVPVRQGAVGVLELTL"
BASE COUNT          454 a          695 c          612 g          325 t
ORIGIN      
        1 ggttcgccac gttgtcatcg acgacaagaa cccgagaccg ttcgccgaca accgccatgg
       61 gcgccacgtt atgccccact agcttgggct tcaatcacca aagttgagag catcagaatc
      121 gtccctctac gcccgtcgtc caggtgaact caccgcgacg gcgttcgagc tcacccaagg
      181 tcccgcgtcg aacgatgaaa tctgcctcgg tccgaaaccg ccagcgctcg tcctcgccca
      241 acgacaacgc ggctctgagc cgacgacgcg cctcctcttc atccacagga ctggacgcga
      301 gctggtcgcc gatgcggctg cgcactgccc aggcacggtc gactcgcgct ccgagccaaa
      361 cttcgtggaa aggcgaggtc caccggaggt gagggctgag agtgaggcca aagccatggc
      421 tatatccgcg atttgccacg accggagtga gctcgtccgc ggtgttggtc tgcaagtaaa
      481 cgggtaaggc gaaggctcca gcctgaacga aggaggcgcc cgcgaccaac cccaattcca
      541 ggcgctggtg accgtaaccg cgccgccaat acgcgaatag ggctggcgcc tccaacacga
      601 agaacggatc cctcggagcc ggagcggcgg agtagcggcg ctccgagtag ttgaccccgg
      661 cccccagggc gagcagggct acggtatttg tcgtcacatc tcggtaagca gaaccaaaca
      721 gtgaacctcg agcaatcacg tcgatctcac gcagctcgtc agcagcggct tgcactcgaa
      781 cgctcaactg cgacagaccg ccgtcgtaga aggcgcgcca cggctcttgc accgtctggt
      841 tcttcgggac gtccaggatc agcgcttcac catgtgccag cagccacccc ctaccatcgc
      901 gcgcgctcca agtgccgctg aaagacgcac ccaagcctag ccgcagtacg tagtcgtccc
      961 gggtcgagaa accactcgag ttgcagcgcc gtgcacgagg taattgcgta ccatcaatgg
     1021 aacgatgcaa gctttcgctt ggagcaaata cggcgcctag aatgccgaac gaggtgctgt
     1081 cacagccgcg caagaacagg cgccccacct gcgtcatggc ttcgccgaaa gcgaaaccac
     1141 caaacggcgt caccatttga tcgttgatgc tgacgcgctc acggaactcg gcgaaaaact
     1201 cccaagccgc agacgcgaaa aacccgacgg ctagagattc ggcaggcgac agatagttgc
     1261 cccgtgcggc caagtagtag agcgtgcccg ccccggagtg agcgagggtg ttggtcttga
     1321 agaaattggt atcgaaggaa taggcatcgc cagtgagctt ggcttcgacg gtgtcccaac
     1381 tgtaatcgaa ggtccagtcc aacgagttca cctcgtgctg catgaagtag tatcccgtcc
     1441 ccgccagcag gagccccact tcctccagcg caatgcgcag cgcacgggac tcgttcgacg
     1501 tatcggactg cttcttggct tcttgcgcag cagcgtcggt tgccaaacca agaacacccg
     1561 aggtaaggca ggccgcgacc accgtcttca gtgatactcg cacgaggcgc ttcgcgaaca
     1621 aggaccgttc caaaggatta cagggtcaat tccaacacgc ccaccgcacc ttgtctaacg
     1681 ggaacgaccg tgggacgaag tcgggacaac aatggttgct cagcgagcgg gtgcggctcc
     1741 tcgggcatcc cggaggtcgc ccaccagctg aaacctagac ccaacaacgc accgccggcc
     1801 gcggctccca tggcggcgcg ggagctcgcc gcgtcgtctg caatgaggag gtaacctcca
     1861 gcccccaata ggccgcccgc caacccgcca agatccacca aacggactcg cgcaacgctg
     1921 ggcgccaact gtggactcgt ggccagcgcc gccaggagag cggcattgta acccagcgcg
     1981 ccgatggcgt aggcgcgctc ggcgcggtaa ctggtttgac tctcggcggc acccgcgatc
     2041 agcgcgcccg cgatgccccc ccaaaggcca cctgacgtgg tgtacg
//
LOCUS       DASTKU010000684         4460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1693567, whole genome shotgun sequence.
ACCESSION   DASTKU010000684 DASTKU010000000
VERSION     DASTKU010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1693567"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..561)
                     /locus_tag="VFQ61_23795"
     CDS             complement(142..561)
                     /locus_tag="VFQ61_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9957551.1"
                     /translation="MVIVKATANSEAGVMPSEKLLTEMGKYNEELVKAGVLLAGEGLH
                     PSSKAKRMLFSGGRKTLVDGPFAETKELVAGFWLWQVKNMDEAIEWARRCPEPMPGED
                     AVLELRPVFEASDFGEEFTPELRAQEENLRARVEQKT"
     gene            complement(606..974)
                     /locus_tag="VFQ61_23800"
     CDS             complement(606..974)
                     /locus_tag="VFQ61_23800"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9957552.1"
                     /translation="MKYLSFVRSPEKFRDAQPPAALMEAMGKLIEKFTKEGALVDTGG
                     LAASREGFRMRLADGTLTTTDGPFTESKEVIGGWAILQANSKEEILRLTTEFMDLHRK
                     YWPGFEGECEVRPIDFQMRG"
     gene            complement(1090..2670)
                     /locus_tag="VFQ61_23805"
     CDS             complement(1090..2670)
                     /locus_tag="VFQ61_23805"
                     /inference="COORDINATES: protein motif:HMM:NF016158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HET9957553.1"
                     /translation="MSAFRRALEDLSGRAASTNSRRWLFVPYDQLSDRIGPLSREPPG
                     SLGIVLVECPAKAARRPYHKQKLALVLANLRSFAIEQASRGVRVRHLVAPAGYDSALS
                     PLAAELGPLHMMEAAERELREELAPLIARGELVVLPHEGWLTSDSDFERASADGFAPW
                     RMDRFYAAVRRRTGILMKKDRPLGGRMSLDAENRKSWRGQPPVPTPPRFEPDSITREV
                     GDLIEQRYAQHPGRLELTMLPATRADAVQLLDWAMSECLPLFGPYEDAMSVRSTSLFH
                     TRLSALINLHRLTPREVLDRALGLHLPLSSQEGFVRQILGWREFVRHVHRRTDGFRMR
                     PTARGQQPLSAREGDPLPAAFWGEASGLACLDRVVADVWSEGYGHHITRLMILSNIAT
                     LLEASPRELTDWFWAAYVDAYDWVVEPNVLGMGTYALGDLMTTKPYVAGAAYIHRMSD
                     YCAECAFDPKKNCPITPLYWSFLARHSDELAGNSRMLVPLSALQKRRPEQRVSDASVF
                     ATVRQALGRGEKLHPGVR"
     gene            3151..>4460
                     /gene="tssI"
                     /locus_tag="VFQ61_23810"
     CDS             3151..>4460
                     /gene="tssI"
                     /locus_tag="VFQ61_23810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HET9957554.1"
                     /translation="MSQTRLVNVVTPLGDELLFLSMSGREALGSPFCYEVELLSESSK
                     VDLSALLGQTITLELELEDGGVREFNGVVTYFSAMGAHGRYALYRATVRPWLALLGNR
                     RNSRIFQDKTVPEVVKEIFRDHGFSDFVESLGPGYEPRTYLVQYRESDLNFVNRILEE
                     EGIYYFFKHQDRKTVLVLADSYSAHEPVPGSETLPYFPPQQREHRERDHIDGWTVFRQ
                     IRSGKVELADFDFEKPAVKLRSNTAMPAEHPHGDAEIYDYPGRFTQRAGGDRLSRLQM
                     EEHLTDQEVAQASGNARAIYSGALFTLQDFPREDQNKEYLVVEYSVELRDNSYESGQA
                     RDTETEYRCRFTAIESKRPYHPPRVTPKPIVEGPQTAIVVGQEGKEIWTDNYGRVKLK
                     FHWDRKEQADQTSSCWVRVAQLWAGSGFGGIHLPRIGQEVIVDFL"
BASE COUNT          843 a         1352 c         1403 g          862 t
ORIGIN      
        1 catctcgctg aaggaatcgt gttccaccat ccggacgggc gtcgagcaaa gatcaaacga
       61 aaggacttct gagcggcaca cactcggaga cccgaaagtg gcatgcacgt ggggcgtcac
      121 gtgcatgcca gagccgttca atcaggtctt ctgctccacg cgggcgcgca ggttctcttc
      181 ctgagctcgt agctccggcg tgaactcctc accgaagtcg ctggcttcga acaccggccg
      241 cagctccaac accgcgtctt cgccgggcat tggctccggg caacgccgcg cccactcgat
      301 cgcctcgtcc atgttcttga cctgccaaag ccagaatcca gcaaccagct ccttcgtctc
      361 ggcgaaaggt ccgtccacga gggtctttct tccgcccgaa aagagcatgc gtttcgcctt
      421 ggaactcgga tgcaagcctt cacccgcgag tagtacgcca gccttcacca gctcttcgtt
      481 gtacttgccc atttccgtga gcagtttttc actcggcatc actccagctt cggagttcgc
      541 cgttgccttg acaatcacca tgactcgcat ttgtctgctc cttctattct cgggttgacg
      601 tgccgtcatc ctctcatctg aaagtcgatc ggccgcacct cgcactctcc ttcgaatccg
      661 ggccagtact tgcggtggag atccatgaac tcagtggtga gccgcaagat ctcctctttc
      721 gagttggctt gcaggatggc ccagccacca atcacttcct tgctctccgt aaaaggcccg
      781 tccgtggtcg tgagggtgcc gtccgcgagt cgcattcgga agccttcacg gctggcggcg
      841 aggccaccag tgtccacgag cgcgccttct ttggtgaact tctcgattag cttgcccatg
      901 gcttccatga gggctgcggg cggctgagcg tcgcggaact tttcggggct gcggacgaaa
      961 ctgaggtact tcattttctg attcctgatt ggggttcgaa taggcgacga acgacggggg
     1021 accagatcga caacccatcc ccattttttc gaatttcggg ctgaggcggc attgtctcgg
     1081 aatcgaggtt taccgaacgc ccgggtggag cttttcaccg cgcccaagag cctgccgcac
     1141 tgtggcgaag accgaggcgt cgctcacccg ctgctcgggc cttcgcttct gcaatgcgga
     1201 gagcggcacg agcatgcgag agttgcccgc cagctcatcc gaatgacgcg ccaagaagct
     1261 ccaatataga ggcgtaatcg ggcaattctt cttggggtcg aacgcgcact cggcgcaata
     1321 atcgctcatg cggtggatgt acgcggcgcc cgccacatac ggcttcgtcg tcatgagatc
     1381 gccgagcgcg taggttccca tcccgagcac attgggctcg accacccagt catacgcgtc
     1441 cacgtaggcg gcccagaacc aatcggtgag ctcgcgaggg gacgcctcga gcagcgtcgc
     1501 aatattcgag aggatcatca gccgggtgat gtgatggccg taaccttcgc tccaaacgtc
     1561 ggccacgacg cgatcgagac acgcgaggcc cgaagcttct ccccaaaacg cggccgggag
     1621 tggatcgccc tcgcgcgcag agagcggctg ctggccacgc gccgtgggcc gcatgcgaaa
     1681 tccgtccgtg cgtcgatgca catggcgaac gaactcgcgc catccgagga tttgtcggac
     1741 gaaaccttcc tggctcgaaa gcggtaggtg caagccaagc gcgcggtcca gaacttcgcg
     1801 gggcgtgagc cgatggaggt tgatcagcgc agaaagacga gtgtgaaaca ggctcgtcga
     1861 acgaacggac atggcgtctt cgtacggacc gaacagcggc aggcactcgc tcatcgccca
     1921 gtccagcagt tgcactgcgt ccgcgcgtgt ggcgggtaac atcgtgagtt cgagacgacc
     1981 tgggtgctgg gcatatcgct gctcgatcaa atcaccaacc tcgcgcgtga tcgagtccgg
     2041 ctcgaagcgc ggtggcgtcg gcacgggcgg ttgccctctc caggattttc gattctctgc
     2101 atcgaggctc atccggccgc ccagcggtct atccttcttc atcaggatcc cggtgcgtcg
     2161 tcggaccgcc gcatagaagc ggtccatgcg ccagggagca aaaccatcag cgctcgctcg
     2221 ctcgaaatcc gagtcactcg tcagccagcc ctcgtgcgga agcacaacga gctcgcctcg
     2281 agctatgaga ggggccagct cttcgcgcag ctcgcgctcc gcggcttcca tcatgtgcaa
     2341 tggtccgagc tcggccgcga ggggtgagag agccgaatca tagccagcag gtgcgacgag
     2401 atggcgtacc cttacgcctc gcgaggcctg ctcgatggcg aaggaacgca gattggccag
     2461 caccagtgcg agcttctgct tgtggtacgg gcgccgcgcc gccttggcgg gacattcgac
     2521 gagtacgatg ccgagtgacc caggcggctc gcgcgacagc gggccgatcc gatccgagag
     2581 ctgatcgtaa ggcacgaaca gccaccgtcg cgaattcgtg cttgctgcgc gcccgctcaa
     2641 atcctccagc gctcgtcgaa atgcgctcat cgctcgagca ccttccccgc gtgggcacac
     2701 ccggtcaacg ggaattagcg ggagtcaggt tgctgtgccc gcagcagaac tcctggttcg
     2761 gacccgccct catatccggg tcgccggggg ggggccgaag aacagggccc gcaaaggggc
     2821 gaggggcaat ccaagctagg cagttccttt gtcgcacggg tggggtcgaa ttgggcgtcg
     2881 aattttctca cgcgatgttt tgaggtcagc ccgtcccgcc ataactgctc ggccaggccg
     2941 gcttccggcg atggccaagg aataggggag attggggctc aactggccgt agcctaggtg
     3001 gcaggctgtg ctagcctgcc ggcaggttgg gcgtgttact gggcagagtc gggcagagtc
     3061 gggctgagtc gggctgagtc gggctgagtc ggatcggtcg gcattgggcc ggatacccgc
     3121 gggtttggtt cttggtgtaa ggatttgcgc atgtctcaaa cgaggctcgt gaacgtcgtc
     3181 actccgttgg gtgatgagct gctgttcctt tcgatgtccg ggcgggaggc gctggggtcc
     3241 ccgttctgtt acgaggtcga gctgctcagt gagagctcca aggtcgactt gagcgcgctg
     3301 ctcgggcaga ccatcacgct ggagctggag ctcgaagatg gcggggtgcg tgaattcaac
     3361 ggagtggtga cctacttctc ggcaatgggc gcgcacggtc ggtatgcgct gtaccgagcc
     3421 accgtgcggc cctggctcgc actactcggc aaccgcagga actcgcggat ctttcaggac
     3481 aagactgttc ccgaggtggt caaagagatt ttccgggacc acggcttctc ggatttcgtg
     3541 gaatcgctgg gcccgggcta cgagccgcgc acctatctgg tgcagtatcg ggagagcgat
     3601 ctcaacttcg tcaatcggat cttggaggag gagggcatct actacttctt caagcatcag
     3661 gatcgaaaga cggtcctcgt cctcgcggac tcttacagcg ctcatgagcc cgtacctggc
     3721 tcggagacgc tcccgtactt cccaccgcag cagcgggaac accgcgaacg cgaccacatc
     3781 gacggctgga cggtgtttcg gcagatccgc tcgggcaagg tggagctcgc cgatttcgac
     3841 ttcgagaaac ccgcggtgaa gttgcggtcc aatacggcca tgcctgcgga gcatccccac
     3901 ggggacgcgg agatttacga ttatcccggg cgtttcactc agcgcgcggg cggagatcgc
     3961 ttgtcgcgcc tgcaaatgga ggaacacctc actgaccaag aggtggcgca agctagtggc
     4021 aatgcgcggg cgatctactc cggagcgctc ttcacgttgc aggactttcc aagggaagat
     4081 cagaacaagg agtatttagt cgtcgagtac tcggtcgagc tgcgagacaa tagctacgag
     4141 tcggggcagg cgagagatac cgagactgag taccgatgcc gctttacggc catcgagagc
     4201 aagcgccctt atcacccccc gcgtgtcacg ccgaagccca tcgtcgaggg gccgcaaacg
     4261 gccatcgtcg tgggtcaaga aggcaaggag atctggacgg acaactacgg ccgcgtgaag
     4321 ctcaagtttc attgggatcg caaggagcaa gccgatcaaa cgagctcgtg ctgggtgcgg
     4381 gtggcacagc tctgggcggg cagcggcttc ggcggcattc atttgccgcg catcgggcag
     4441 gaggtgatcg tcgacttcct
//
LOCUS       DASTKU010000685        22890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_799711, whole genome shotgun sequence.
ACCESSION   DASTKU010000685 DASTKU010000000
VERSION     DASTKU010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 22890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22890
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799711"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFQ61_23815"
     CDS             complement(<1..339)
                     /locus_tag="VFQ61_23815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957555.1"
                     /translation="MPLALRAAGGGFLADFDEWFSRVGEGTNGSQEAGHGRHRDAAGT
                     FRGLSFADDAAGVVDESFEPASDGLSERESARAAGPYPVDKREAGEATVQAGFVCACQ
                     TPVHPVRCRPT"
     gene            733..1368
                     /locus_tag="VFQ61_23820"
     CDS             733..1368
                     /locus_tag="VFQ61_23820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylated adapter RNA export RNA-binding
                     domain-containing protein"
                     /protein_id="HET9957556.1"
                     /translation="MATEDTVSNIAKSLGEADEIPLTQIAGIVRVLGEEGALALLNET
                     QEIEKGGGMLLADGTRRRSPGGVFFQLARRKLPIEERKNIFREKKPAGEAPPPSSRPG
                     AQPQTAAATAADATRFPRRRVVEVVPARAAGPARGAKGFTPPELPSALTRARIRQQVT
                     QAVAPLPLEEQYHLLLDLLADIHDRATGGANPPPSSEDALPSRARSARRTK"
     gene            complement(1592..2179)
                     /locus_tag="VFQ61_23825"
     CDS             complement(1592..2179)
                     /locus_tag="VFQ61_23825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9957557.1"
                     /translation="MLGACQVHHLHEVKEASPRRLQPEREREIRAALVRHLPQLTAHA
                     VRLTKDTIEAHDVVQEAALRALNFSWSYQAGSNARAWLHQILDSVFMTRCRRRAREKR
                     AFRALTDDPNAWLKNGSNGSETRRLSPRVESALQLMPGSFRDVVYLVDVHGLSYKDAA
                     ETLRVPLGTVMSRLHRGRRLLAAELSDSTLSARAA"
     gene            complement(2276..3844)
                     /locus_tag="VFQ61_23830"
     CDS             complement(2276..3844)
                     /locus_tag="VFQ61_23830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET9957558.1"
                     /translation="MLRILSREQLRAFDRLCAEQCSVPPLSLMENAGRGAADWIAARY
                     QRGRFKAKAGASRPERVLVVAGRGNNAGDGYVVARRLQLLGVPVEVWALYGEAELSGD
                     ALLNYRAAQASGVAVHHAAGANADELISRASWVVDAVLGTGLSREVGSPEREAIAAMN
                     AARAHKIALDVPSGVHADTGAVMGLAFHADYTLTFAAHKLGFFTAAGLEHVGGVYLMD
                     IGVPASAVRESGESALLVEPQDVRSTLRMRSARAHKVSAGRVAIVAGSPGKLGAALLV
                     ARGAARAGAGLVTLVADGALAEAFDLRVLEAMTARFEPSDLPGSLERCLEGADVVVIG
                     PGLGFESQAARCIEHVVLHEPRPVVVDADAISYFRGAPERLAAARGPRILTPHTGELA
                     RLLGRTSAEVERERWRATAQAAAATRAIVLLKGPHTLVAAHDQTARVGRRGSHVLATG
                     GAGDVLGGVIAALACELDPFDAAWVGAYLHARSGELWAKQRGADRGLLAHEIADGIPD
                     ALAELSVRHGRLTL"
     gene            complement(3852..5147)
                     /locus_tag="VFQ61_23835"
     CDS             complement(3852..5147)
                     /locus_tag="VFQ61_23835"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9957559.1"
                     /translation="MSDGTGRKRRLTLTAIGVGGLAVMVVAALLQVRRGAPRNADPSP
                     RENPADNKGSPHAAGASQTPAPAPTRKPADPPAPALSAPARPPAEVAFPGLESKDLRE
                     QKAALLGLMQRELELDEGSVAKVRTIYEGSSWMSQGNPKVTQHPMTRAECRARRASAE
                     PLPSGDSVCGKPNMVALYDPSRGETVNDARACIDQYEFPNIPCEYPVIWVKADEAVEL
                     CKAEGKRICDAHEWEGGCAGALKKPEEEYAFGQRRLMIEYLHNKERDVVWSYGKTKDH
                     SKCATGSRKSTTCNVIDWDLCGSNTYPAGSFPECRSPFGAFDLNGNAAEHMNLPLTPE
                     QLASRGGLGETEMKGSWFIFAMYEAHEDDCRWRAPMWHKSKIDARGSHRNYHLGFRCC
                     SDVKSVSDSAPPAVSAAPPSSVAPVGSAEPTRSPVQPGK"
     gene            5125..6459
                     /locus_tag="VFQ61_23840"
     CDS             5125..6459
                     /locus_tag="VFQ61_23840"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957560.1"
                     /translation="MRPVPSLTGFSLVTSLHDPVILPDHFDSATAARGRDPSDLALPT
                     CAIRSAELLQRAVSHPLKVAACPHRPACPGCPRFGSAALAPDAAKTLQRLAELGGLAA
                     PLVHRGAPLGFRYRARLAIRGRAKNPKLGIFRIGTHQVVHIPHCPIHHPAINSAAQVI
                     RSALVQHGVAPYSDSAHAGSVRYLQLVVERRTGRVQVVVVTRDDDHLALAPFFATLVS
                     SLGDSLQGLFWNIQPERSNAILGPEFRHLAGETEIREDFAGARLFFPPGAFGQSHLDL
                     AERIALGVAEACPRGARVAEFYSGVGALGLPLVRQVSRLTCNEISDASIDGLQLGIRA
                     LEPELAERVQLRPGSAGSATDLLGQVDLVIVDPPRKGLDRALLEGLLRIPPARLVYVS
                     CGLDALVREAQELLSSQLWQLSRLEAYDLFPYTEHVETLAIFDRRGAHGSRA"
     gene            complement(6544..9672)
                     /locus_tag="VFQ61_23845"
     CDS             complement(6544..9672)
                     /locus_tag="VFQ61_23845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5808 family serine peptidase"
                     /protein_id="HET9957561.1"
                     /translation="MRILERLGRALVVLGVFGVASYFALHFGWGGLWTGLEAAHAVTS
                     PGAPKAPYDLTQLTAVNETLNTIRNKYVDPSRVKPRQMFLSALNQIQKEVAQVMLHYE
                     DKAATVKVTVDTESAEFRVDNVQGPWDVAARLREVFAFLQANLKDSGVDLREVEYAAC
                     NGMLRTLDPHSTFMSPEAYREMTLSTSGHFGGLGIVISIRDQQLTVMRPMPGTPAGRA
                     GLKRFDRITKINNESTLNMPLDDAVNRLRGKPGSKVTVWLHRDGSEGWQGARPFELTR
                     EEIQVRSVDSRALAPGVGYIRLKQFQASSSDELDDALNELSKKERLRGLVLDLRNNPG
                     GLLDQAAKIADRFLDDGVIVTTVGNSEGRDEKRAARKGTEPNYPIVVLVNEASASASE
                     IVAGALKNQDRSIIVGQTTFGKGSVQLVFPRITPENAALKLTIAQYLTPGDVSIQGVG
                     VAPDVELDPMTADTLEMDLFRSERSFRERDLMKSLTSAARRSTEQPFFKLRYNLPEAE
                     RAAIRERGGDMEDEFELDFPIKVARDLVSKLAPGKRLEQLRSLKPQLDKLQQAEIDAV
                     SHDLAKMSIDWNGPPKDYSNGLKASDYEVKVQTDRAGDTVNAGEVMNLRVSVRNKGNT
                     PIYQLRAITKSDGPYYDERELIFGRIDPGQTRTASVPFGFCDVDGRKPGSTKPVGENA
                     PRVCKIPLDANTRSDVVIVRFFAEGAEPPRDVELRPTVNALQQPIFAYTYQVADNRPG
                     NGDGQIARGEGATVYLTVKNVGKGTSYETQANLRNLTGDGLLLHAGRFDVSNMKPGET
                     RSVAFTFDVLDSLADNMVNVEITVVDRDLKEFSSEKLKLPIASNALSLQGASGRVTAT
                     AAAPLRGQPLDAAPIVGVVEKGAVLDRSASFGRFTKVLLGEDRFGFVETRLLKDGTGP
                     VKPQFKPSLTHSPPLLEVASAKLATREDKVRIEALAADSDRVMDAFVFVGPRKVFYQS
                     NRKAQDATKLKIGFDAQLEPGVNVITVVARENEDTITRSTTIVRRDGPNGEPLPTPKA
                     ESHDDWESSDGD"
     gene            complement(9822..11054)
                     /locus_tag="VFQ61_23850"
     CDS             complement(9822..11054)
                     /locus_tag="VFQ61_23850"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET9957562.1"
                     /translation="MAFAREVKVGLFVMAGLAVSAMVIFMIGEERRAFGSKETYFSVF
                     KDVQGLRRGSPVRMGGVDIGSVADVSYGENAKDASIFVKMSIVAGEARRIRKDSISSI
                     EGKGLLGDKMIVISVGNPSLPQIPPGERIESKTGDDMSVMITRLGAVSAQAERVMGNL
                     ERTTGMLADPELHDDLKASVGSLANILGAVERREGYVGRLFGDPAEAERLSRTFAHLE
                     QVSGELTTTTRNINQIIGQIQHGPGLAHEVVYGNEGQRAVAQLGQAAEELGTTLKGVR
                     EGNGLARSLLFGDARGDRFAADLNDVSHDLKQIVADLKAGRGTLGALLVDPSVYEDLK
                     LVLGNVERNKALRALVRYSIRQEEASPKVSDPDAPSNARSGTGQNQNSAPREASRAAG
                     SGLRASAGQPSQAPAAAE"
     gene            complement(11283..12062)
                     /locus_tag="VFQ61_23855"
     CDS             complement(11283..12062)
                     /locus_tag="VFQ61_23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9957563.1"
                     /translation="MPEPLIRFQDVRKAFGPKVIYRGLNLEIAKGEVLTIVGGSGVGK
                     SVMLKMLIGLLRADHGSILFDGQEVTQMKEESLADVRKRIAMLFQGAALFDSLTVGEN
                     VAYGLEEHFRQKMSKQERDERVAWALGLVGLPGIELMRPADLSGGMRKRVGLARAIAV
                     QPEVLLYDEPTTGLDPINTARVNHLIMGLQEKLHITSIVVTHDMKSVFTISDRVAMVH
                     GGRIIQVGNVDEFRSTKDPRVSDFVEGRAPVKESVETLLTS"
     gene            complement(12166..13878)
                     /gene="recJ"
                     /locus_tag="VFQ61_23860"
     CDS             complement(12166..13878)
                     /gene="recJ"
                     /locus_tag="VFQ61_23860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET9957564.1"
                     /translation="MLSSAPSVVQVTRRPTGPSGEVSRIAAELGVSYTFAAWLRQRGF
                     DASGKTRDFLNPKLSGLTLPDGMADRDVAAQRLARAVRAREKIVVFGDYDCDGMTATA
                     ILTEILRMLGGDVTPLLASRFDGGYGLSRSAVQRIQRERPGLLVTCDCGSSDHVALEE
                     LSRSGVQAIVVDHHLVPEAPLPALAFLNPHRKDCGFAYKGLASCGLVLSLGGAVRRAL
                     DKKLELKAWLDLVAIGTVADVAPLDGDNRALVRAGLHVLAAPERPGLKALLSEARIGG
                     GGALTAEDIAFSIAPRLNAPGRLGAPDVALALLLSRSESEAYALAARLEQASAERKAQ
                     QEKLQDEALDEIAREGYAERSSLVLGRAGWNHGIVGIVAGRLADSFARPVVVCAIEGG
                     RARGSVRGPAQARLHDLLQACSPLLERFGGHQAAAGLELRSEKLPEFREAFEAATRAQ
                     AAVAAVRPVEPAEDAASVWLDLQDSPDRVLRDFAMLEPCGRDNPTPRLLVETRVLAAR
                     AVKGGHLKLELELSSGQKLSGFGPRLGDLSERLGATALLCGRLRRDRYRGGDALELHV
                     ESVL"
     gene            complement(14047..14433)
                     /locus_tag="VFQ61_23865"
     CDS             complement(14047..14433)
                     /locus_tag="VFQ61_23865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957565.1"
                     /translation="MHRLSAAVPSASRPLPLTLPGELWGTLSPAQQAVYRRAESLLDA
                     FTDSRQLALSRLMQLGPRAEVLRALQVLGGMSLIEIDNAEEDPQVRLRATPEEHLRVV
                     GPDGRPRWIFVARPIDPPSVESGLLN"
     gene            complement(14541..15773)
                     /gene="secF"
                     /locus_tag="VFQ61_23870"
     CDS             complement(14541..15773)
                     /gene="secF"
                     /locus_tag="VFQ61_23870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET9957566.1"
                     /translation="MHLFKSHKVYDFMSVRRYFIALSLFLTVFSLVLVTGKVPGIEPR
                     FGTDFKGGTEIEVAFHAPVGPGEVRDAVQRAGFSTPDVVRVEDGKQQNHYLIRVQEVS
                     TLSAETQAEVEKALCLTPGLPEAACPADKRATEVKFSPGGDKITARFREAPDLDWVRE
                     HVSAVKGIRLRPGGNNPFIQNARDQKVEIQLMSKGDQLVAGLKRALGAKAPDQPLRSE
                     WIGPRAGAQLRDSAVKSVAISIVFIMAYIAFRFDLRFAPGGVVALIHDAVGMVGVLIL
                     LGREINLTTVASVLTIIGFSVNDTVVIYDRVRENLGKLRGASFRHLINLSTSEMLGRT
                     LLTNATVQISLIAFFVWGTGTLKEFALALTVGMVLGTYSSIYVALPLTEWLDRVLFQR
                     IGSSRGGASVDKGATAAV"
     gene            complement(15805..17913)
                     /gene="secD"
                     /locus_tag="VFQ61_23875"
     CDS             complement(15805..17913)
                     /gene="secD"
                     /locus_tag="VFQ61_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET9957567.1"
                     /translation="MAPNVILTIVFGACAALSLLLGAVSRPRRVLAFGATLMLGGAAG
                     AAWVDGFWAMVALALIGFWLLFMTTELVDAGWRLRAGIAIGICGLAFLVLWPTISEST
                     GGKLPCPAYVKEKVDFRLVAGLDLRGGLRLVYTVDVDEAIKDKRDHYYEDMRLELSKL
                     LGLHKGDELPSDEIFQKLREKVELEAPRRPANMIRMKLKEGFDPGKIDDRFLERFRSE
                     LSYVRQDPRTYEFQIRSAVETQIRERAVGQAREIIHRRVDELGLREAAISTRDEDIII
                     EVPGSDEKSFANIREIISQTARLEFKLLDDDTDFFGQVRSTAKEESLPEGLEFYSENV
                     SVGKNAQGDVQTRQNTFAFLKKLPKETSQQTLQRFREWVATLPVPPDREVGYEIEYRL
                     VDELTGKQEENGFRSYLLKSRAEVTGDMIRDAAAVPDQSQTSMGSWYVSLTFTDQGGK
                     IFERITANNIKRRFAIILDNRVESAPVIQTRIPGGHAQITLGSSDPEIQLRDARKLEL
                     VLRSGALPAPISPSNEQRIGPTLGRDAIELGVQGALGGIVLVLMFMLIYYQRAGLIAD
                     FAVLVNLFLQLAILCMFGAAMTLPGIAGLSLTVGMSVDSNVLINERIREEMALGKSAR
                     AAVEIGYRRAFSAIVDGHITTVIAGVVLAQYGTGPLKGFAVTLIVGIVCNVFTGVVVT
                     RLFFEAWIRMLGRHGRLDMG"
     gene            complement(17943..18512)
                     /gene="yajC"
                     /locus_tag="VFQ61_23880"
     CDS             complement(17943..18512)
                     /gene="yajC"
                     /locus_tag="VFQ61_23880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET9957568.1"
                     /translation="MSLAASAPGNGSHSVASEVVVSEVHLASKPGVVTSEVAIHSKAH
                     APAGPVSHGVGGAKSSAFLLSAYQQVPPAPGREPSVSQPAPSAPAGAAPPPSPLGGIL
                     PLLIMVVPMVLLLVFSSRSQQKKQAAAIASLKKGDRVLIQGGLVGRLVELTDRYAKLE
                     IAPGVKVEVLKSSVLGADNVETQAAAEKK"
     gene            18885..18956
                     /locus_tag="VFQ61_23885"
     tRNA            18885..18956
                     /locus_tag="VFQ61_23885"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18917..18919,aa:Val,seq:gac)
     gene            19608..20360
                     /gene="infC"
                     /locus_tag="VFQ61_23890"
     CDS             19608..20360
                     /gene="infC"
                     /locus_tag="VFQ61_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET9957569.1"
                     /translation="MALSGGGAGGRRFSRDNRGPQIRVNHRIRVPEVRVVAEDGSNLG
                     VLSTEQALRRAQEVGLDLVEVNPKAVPPVCKILDFGKYKYEEKKKQREAKKKQTVVEV
                     KEIKIRPKTDDHDVEVKIRAIRKFIESGNKVKVTCRFRGREIMHPERAQMQLNLILSR
                     VEDIANVEQRPVMEARTMALVVAPKPVVFQRLQSDRAQRERERERQIAQGQVPTAEEE
                     EDFEDEDLDDEDDDDDDEDEDEDEDEDDSAKA"
     gene            complement(20524..21957)
                     /locus_tag="VFQ61_23895"
     CDS             complement(20524..21957)
                     /locus_tag="VFQ61_23895"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957570.1"
                     /translation="MAQQQQRYRVIEKIASGGMAEVFRAESAGLEGFKKTVAIKRVLP
                     HLAEKKQFIGMFLDEARLSAHLSHSNCVQVFDIGVGDNTYFIVMEFVDGSDLKAVIEY
                     RRRIGQPIPIEEACLICVRICEGLAYAHELSDSKGESLKIVHRDMSPPNVLITRHGEV
                     KIVDFGLAKANSQLEHSEPGIIKGKSSYLSPEAAQGLPVDARTDIFAVGIILWELLAG
                     QRLFLGESDLETVRMVQQARVPSVRKYNPRVTRELEDVLMRSLAGDPSARYSSARELG
                     RDLNKLLFHLGREVSSFDISQLVLPIWRERLEKKRQKAAGPVGRGSIIGSLIDEALFE
                     FTSIDGGDKKSQVSMVGAMPLSLGTFAQQNWMDDLGLSKAENPTPEPLEVGNLAALED
                     DPISAHSPSAPPHSSPPNASSAPPAGVSAPPPTGPSVPAPMDPSSDDDEAPFARKKTN
                     PGLIVGVVFAILLILALVAYFAGAFRR"
     gene            22194..22610
                     /locus_tag="VFQ61_23900"
     CDS             22194..22610
                     /locus_tag="VFQ61_23900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957571.1"
                     /translation="MSAHDSLAGGAHGRHEGHDHPGAAHAHGAHGHDHAGHDDDHGGH
                     DDHHVEPPPPEPESPAWLPLLGGGLFILGLVVYLLMQSNPSAPSPAGATGEKPVAGAA
                     AAKGAPDAAPPSGGVPRPAMADRPNRPMPAQAPAGH"
     gene            22695..22767
                     /locus_tag="VFQ61_23905"
     tRNA            22695..22767
                     /locus_tag="VFQ61_23905"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22728..22730,aa:Ala,seq:ggc)
BASE COUNT         4255 a         7614 c         6917 g         4104 t
ORIGIN      
        1 agtgggcctg caccgtactg ggtgcacggg agtctggcag gcgcacacga atcccgcctg
       61 gaccgttgcc tcgcccgctt cgcgtttatc cacagggtac gggccggccg cgcgagctga
      121 ctcgcgttca gacagcccat ccgaggcagg ttcgaacgac tcgtccacaa ctcctgcggc
      181 gtcgtccgcg aaggataacc cccggaatgt accggctgca tcgcggtgtc tcccgtgccc
      241 cgcttcctgg ctcccattcg tgccctcccc aaccctcgaa aaccactcgt cgaagtctgc
      301 cagaaagcca ccgcccgctg ctcgcaacgc gaggggcata acggtggcag cctgaaccat
      361 ccctttcggg tacaacagcg gtacggcgaa gcccggacta cggaagcccg gactacggaa
      421 gcccggacta cggcgaagcc cggactacgg cgaagccctg gggactccag cgcagtatca
      481 cgcggcaaac aacgcagccc agactaccgc gcacccggag tacggggggc gaactaccgc
      541 cagctctggc cccggtgact accaggaatg aggctgcccc aaacaggtag tagcggggga
      601 agggagctga attttccgga tggatccgct aagctgtttt ggaattgcgc gggcgagttg
      661 cagtctcgct cggcgcgagg cacagtccga accccaccca cgaccggggg cgtggcccag
      721 atccaatcaa acatggcaac tgaagatacc gtctcgaaca tcgcaaagag tctcggagag
      781 gccgacgaga tccctctgac tcagattgct ggcatcgttc gcgtgttggg cgaggagggt
      841 gccctagcac tgctgaacga gacccaggag atcgaaaaag gcggaggcat gttgctcgcc
      901 gatggcaccc gccgcagaag ccccggcggc gtgttctttc aactcgcacg gcgcaagctc
      961 ccgatcgaag aacgcaagaa catctttcga gagaagaagc ccgccggcga agcgcctccc
     1021 ccgtcttctc gacccggagc gcagccgcaa accgccgccg ctaccgctgc ggacgcaact
     1081 cgctttccgc gtcgtcgcgt ggtcgaggtg gttcccgcgc gtgccgccgg ccctgctcga
     1141 ggagcgaaag gttttactcc tccggagttg ccgagcgcac tgacgcgcgc tcgtatccgc
     1201 cagcaggtga cccaggccgt cgccccgctg ccgctcgaag agcagtacca tctgctgttg
     1261 gatctcttgg cagacatcca tgaccgagct acgggcggcg ccaacccacc gccatcatcc
     1321 gaagacgcgc ttccgtcacg cgcccgcagc gcgcggcgca ccaagtagcg cagactcgcg
     1381 ggttagaact gagtcgtccg ggccccacac cagggcccgc ggggtgcggt ggatctcgct
     1441 ggatgcgctc ttgcgaactc gcgtcgttac ggacgccgct gctccggaca ttccgggcca
     1501 ccgatcgctg agtgccgtga agtacgtgaa gggctgctgc cgcaacggtg ggcgcgcaag
     1561 ctcatgcccc ttctcagtta gcgcgttagt ctcacgcagc gcgtgcagag agggtcgagt
     1621 cagaaagttc tgccgcgagc aagcgacgcc ctctgtgtag acggctcatt accgtcccca
     1681 ggggtacacg caacgtctcg gcggcgtctt tgtacgagag cccgtgcacg tccacgagat
     1741 acaccacgtc ccgaaagctt ccgggcatga gctggagcgc gctctccacg cgtgggctca
     1801 ggcgtcgtgt ctcggagcca ttggatccgt tctttagcca agcattggga tcatccgtca
     1861 aagcccggaa ggctcgcttc tcccgagcgc ggcgacggca acgcgtcatg aagacgctat
     1921 ccagaatctg gtgcaaccag gcgcgagcgt tgctgcccgc ctgatagctc caggagaagt
     1981 tcagtgcgcg cagcgcggcc tcctgaacga cgtcgtgagc ttcgatagtg tctttggtga
     2041 gccgcacggc gtgtgcggtg agctgcggta agtggcgaac caacgccgct cgtatttctc
     2101 gctcgcgctc cggctggaga cggcgcggcg aggcttcctt cacttcgtgt agatgatgaa
     2161 cttggcaagc acccaacatg gccagccctc atcgcaagca acatgccacg cacgcttcag
     2221 ccaaattagg ctcgatcagc ctcgagtact cggacaacca gtcaggaagc tcttgtcaaa
     2281 gtgtcaggcg gccatggcgg actgacaact cggcaagcgc gtccggaatc ccgtcggcaa
     2341 tctcgtgagc gagcagcccc cgatcggctc ctctctgctt cgcccaaagt tctccactcc
     2401 gcgcgtgtaa gtaggcgccg acccacgccg cgtcgaatgg gtcgagctcg cacgccaatg
     2461 ccgctatcac gcccccgagc acgtcgcccg ctcctcccgt cgccaggacg tgggatccgc
     2521 gacgcccaac ccgagcggtt tggtcgtgag cagccaccaa tgtgtgaggg cctttcagca
     2581 atacgatcgc tcgggtcgct gcggctgcct gcgcagtagc tcgccaacgt tctcgctcca
     2641 cctcggcgga ggtgcggccg agcaaacgcg cgagttcccc ggtgtgaggg gtcaggatgc
     2701 gcgggccacg cgcagcggcc aagcgctcgg gagccccgcg aaagtagctg atggcatccg
     2761 cgtccacgac gacggggcgc ggctcgtgca aaaccacatg ctcgatgcag cgcgcagcct
     2821 gggattcgaa gcccaagccg gggccgatca ccacgacatc cgcaccttcc aagcaacgct
     2881 cgagcgaacc cggcaaatcc gacggctcga agcgcgcggt catggcctcg agcacccgca
     2941 agtcgaacgc ttcggcgagc gccccatcag ccactagggt gacgagcccg gcccctgctc
     3001 gcgcggcacc tcgcgcgacc agcaatgccg ccccgagctt accaggggac ccggcgacga
     3061 tggcgacgcg ccccgccgat accttgtgcg cacgcgccga gcgcattcgc aacgtcgaac
     3121 gcacatcctg aggctcgacc aacagcgcac tctcgccgga ctctcgcacg gcagatgccg
     3181 gaacgccgat gtccatcaag tacactccgc caacgtgctc gagcccagcg gccgtaaaga
     3241 agccgagctt gtgcgcggcg aacgtcagcg tgtaatcggc gtggaatgcg agccccatca
     3301 ccgcgcccgt atcggcgtgt accccggagg gtacatccag cgcgattttg tgagcccgcg
     3361 cggcgttcat agccgcaatg gcttcgcgct ctgggcttcc cacctcgcga gacagccccg
     3421 taccgagcac cgcgtcgacc acccaactcg ctcgcgatat cagctcgtcg gcgttcgcgc
     3481 ccgcagcgtg gtggaccgca actccgctcg cttgcgccgc gcggtagttg agtagtgcgt
     3541 ctccgctcag ctccgcttcg ccgtacaagg cccaaacttc gaccggaact cctagcaact
     3601 ggagccgtcg cgcgacgaca tatccatccc cagcgttgtt gcctcgcccc gcgaccacca
     3661 gcacgcgctc tgggcgggat gctccggctt tggccttgaa gcgcccgcgc tgatagcgag
     3721 ccgcgatcca gtctgccgct ccacgacctg cgttctccat cagagagagc ggcggaacgc
     3781 tacattgctc agcgcacagt cgatcgaatg cccggagttg ctcgcgcgaa aggatccgca
     3841 acatacgaag ctcacttccc cggctggacg gggcttcgag taggctctgc gctgccaacg
     3901 ggagccacgc tactcggcgg cgctgcgctc accgcgggtg gcgcactatc cgagacggat
     3961 ttcacgtccg agcagcagcg aaagcccaaa tgataattcc ggtggcttcc gcgcgcgtcg
     4021 atcttgctct tgtgccacat gggcgcccgc cagcggcaat cgtcttcatg agcttcgtac
     4081 atggcgaaga tgaaccagct ccccttcatc tccgtctcac ccagcccgcc gcggctcgcg
     4141 agctgctctg gcgttagagg caggttcatg tgctcggccg cattgccgtt caagtcgaac
     4201 gcaccgaacg ggctgcgaca ctcgggaaac gaaccagccg ggtacgtatt cgagccgcac
     4261 aaatcccagt cgatcacgtt gcaggtcgta ctctttcggc tgccggttgc gcatttcgag
     4321 tggtccttgg tcttgccgta ggaccaaacg acgtcgcgct ctttgttgtg caggtattcg
     4381 atcatcagcc gacgttgtcc gaaggcgtac tcttcttccg gcttcttgag ggcccccgcg
     4441 cagccgcctt cccactcgtg agcatcgcag atgcgcttac cctcggcctt gcatagctcg
     4501 actgcctcgt ccgctttgac ccaaatcacc gggtactcgc aagggatgtt gggaaactcg
     4561 tactgatcga tgcaagctct cgcgtcgttt acggtctccc cgcgcgacgg atcataaagc
     4621 gccaccatgt tcggctttcc gcagaccgag tcgccgctcg gcaggggctc ggcactcgcg
     4681 cgacgggccc gacattccgc gcgcgtcatc ggatgttgcg tcaccttcgg attgccttgg
     4741 ctcatccaac tcgagccctc gtagatcgtc ctcaccttcg caacgctccc ctcgtcgagc
     4801 tccagctcgc gctgcattag cccgagcagc gctgccttct gttcccgtaa gtccttggac
     4861 tcgagacctg ggaacgccac ttcggccggc ggccgcgcgg gcgcgctcag agctggcgcc
     4921 ggcggatcgg caggcttacg ggtcggcgcg ggcgcgggcg tctgactcgc ccctgccgca
     4981 tgcggtgacc ccttgttgtc ggcagggttc tcccgtggcg atgggtctgc attccgaggc
     5041 gccccgcgcc tcacctggag cagcgcggcc acgaccatca cggcaagccc accgactccg
     5101 attgcagtga gcgtgagccg gcgtttgcgg ccggttccat cactcacggg attctctctc
     5161 gttacgagcc ttcacgaccc ggtcatctta ccggatcact tcgatagcgc cacggccgcg
     5221 cgaggtcgtg atcccagcga tttagcgctg ccgacgtgcg caattcgctc cgcggagcta
     5281 ctccaacgcg ccgtgagcca cccgttgaaa gtcgccgcct gcccacatcg gcctgcttgc
     5341 cccggttgtc cacgcttcgg ttcggccgcg ctagcgcctg atgcagccaa gacgctgcaa
     5401 aggttggcag agctcggtgg cctcgcggcc cctctcgtcc accgcggggc gcccctcggt
     5461 tttcgctacc gagcgcggct cgcgattcgc gggcgcgcca aaaacccgaa gctcgggatc
     5521 tttcgaatcg gcacccacca ggtcgtgcat atcccgcatt gcccgattca ccacccggcg
     5581 atcaattcag cggctcaggt gattcgaagc gcgctcgtcc aacatggcgt ggccccgtac
     5641 tcggacagcg cacacgcggg cagcgtacga tacctgcaac tcgtagtcga gcgtcgcacg
     5701 gggcgcgtac aggtcgtagt cgttacgcgt gacgacgacc atctcgccct cgccccgttt
     5761 ttcgcaactc tggtgtcgag cctaggcgac agcttgcaag gtctgttctg gaatatccaa
     5821 cccgagcgca gcaacgcgat cctcgggcca gagttcagac accttgccgg cgagacggag
     5881 atccgtgagg actttgcggg tgcgcggctg ttcttcccac ccggagcctt cggtcagagt
     5941 cacctcgatc tggccgaacg gatcgcgctc ggtgtagccg aggcatgccc ccgaggagcc
     6001 cgggtcgcgg agttttatag cggagttggc gcgctcgggt tgccgctcgt gcgtcaggtg
     6061 agtcggttga cctgcaacga aatctccgat gcatccatag atgggctcca actcggaatc
     6121 cgagcgttgg aaccggagct cgccgaacga gtccagctgc gaccgggcag cgccgggagt
     6181 gccacggact tgctgggcca ggtcgatctc gtcatcgtag atccgccgag gaaaggccta
     6241 gatcgcgctt tgctcgaagg tttgctgcgc atacccccgg cgcgactcgt ctacgtcagc
     6301 tgcgggctcg acgcgctagt gcgtgaggcc caagagctct tgagctctca gctctggcag
     6361 ttgagtcggc tcgaagctta cgacttgttc ccctacacgg agcacgtcga gacgctcgcg
     6421 atcttcgacc gccgaggcgc gcacgggagc cgcgcgtaag cgcccttcca ctcggagggc
     6481 cgcagcagcg cggttaactt cgcgcggcta cgaccccaga gtgattcggc agttcaggcg
     6541 cgctcagtca ccgtcgctgg attcccaatc gtcgtgggac tcggccttgg gcgtgggcaa
     6601 aggctcgcca ttcggaccat cccggcggac gatggtcgtg gagcgcgtga tggtgtcctc
     6661 attttcacga gccaccaccg taatgacgtt cactcccggt tcgagctgtg cgtcgaaacc
     6721 aatcttcagc ttggtcgcgt cctgagcctt acgattcgac tgataaaaga ccttacgcgg
     6781 cccgacgaaa acgaaggcat ccatcacgcg atcgctatcc gccgcgagcg cttcgatgcg
     6841 aaccttgtct tcgcgcgtcg cgagcttggc cgacgccacc tcgagcagcg gcggagagtg
     6901 ggtcagcgac ggcttgaatt gaggcttgac cggcccggtg ccgtccttca gtagccgcgt
     6961 ctcgacgaag ccaaatcgat cttctccgag cagcaccttg gtgaagcgtc cgaagctcgc
     7021 gctgcggtcc aacacggcgc ccttctccac cacgcccacg atcggcgctg cgtcgagcgg
     7081 ctgcccgcgc agcggtgcgg ccgcggtcgc ggtcacgcgc cccgaagcgc cctgcaagct
     7141 caaggcgttg ctcgcgatgg gcagcttcag cttctcactc gagaactcct tcaaatctcg
     7201 gtcgaccacg gtgatctcga cgttcaccat gttgtccgcc aaactatcga gcacgtcgaa
     7261 ggtgaacgcc acgctgcgag tttcgcccgg cttcatgttc gagacgtcga agcgcccggc
     7321 gtgcagcagc aaaccgtcgc ccgtcaggtt tcgcaggttg gcctgcgtct cgtaagacgt
     7381 gcccttcccc acgttcttca ccgtgaggta cacggtcgcg ccttcaccac gcgcgatttg
     7441 accatcccca ttaccgggac ggttgtccgc cacctggtag gtgtacgcga agatgggctg
     7501 ctgcagagca ttgacggtgg gccgcagctc gacgtctcgc ggcggctccg cgccctccgc
     7561 gaagaagcgg acgatcacca cgtcggaccg cgtattggcg tcgagcggga tcttgcagac
     7621 gcggggcgcg ttctccccga ctggcttggt ggaacccggc tttcgaccgt ccacgtcaca
     7681 gaaaccgaac ggcaccgaag ccgtgcgtgt ctgccccggg tcgatgcgcc cgaagatcaa
     7741 ctcgcgctcg tcgtagtacg ggccatcact cttggtgatt gcccgcagct gatagatcgg
     7801 cgtgttgccc ttgttgcgca cggagacccg caggttcatc acctcgcccg cgttgacggt
     7861 gtcgcccgcg cgatcggtct ggaccttcac ctcgtagtcc gaagccttca gaccgttgga
     7921 gtagtccttg gggggcccat tccaatcaat cgacatcttc gcgaggtcgt ggcttaccgc
     7981 gtcgatctcc gcttgctgca gcttgtcgag ctgtggcttc aacgaccgca gctgctcgag
     8041 gcgcttgcct ggtgcgagct tcgagaccaa gtcgcgcgcc accttgatcg ggaaatcgag
     8101 ctcgaactcg tcctccatgt ctccgcctcg ctcgcggatc gccgcgcgct cggcttcggg
     8161 caggttgtaa cgcaacttga agaacggctg ctcggtgctg cgtcgagccg cactcgtcag
     8221 gctcttcatc agatcacgct ctcggaacga ccgctcggat cggaacaggt ccatttccag
     8281 cgtgtctgcc gtcatggggt cgagctccac gtcgggagcg acgcccacgc cttggatcga
     8341 gacgtcaccc ggcgtcagat actgcgcgat ggtcagcttc aaagccgcgt tttcaggggt
     8401 gatgcgcggg aaaaccagct gcaccgaacc tttgccgaac gtggtttgcc ccacgatgat
     8461 cgagcggtct tggttcttga gcgctcccgc cacgatctcg ctcgcgctgg cgctggcttc
     8521 gttcaccagc accacgatcg ggtagttcgg ctccgttccc ttgcgcgcag cgcgcttctc
     8581 gtcgcgaccc tcggagtttc cgaccgtcgt gacgatcacc ccgtcgtcga ggaagcgatc
     8641 cgcgatcttc gcggcctgat cgagcaggcc gccaggattg ttccgcaaat ccagcaccaa
     8701 accgcgcaac cgttccttct tgctgagttc gttcagcgcg tcatcgagct cgtccgagct
     8761 cgatgcttgg aactgcttga gccgaatgta gccgacgccg ggcgccaacg ccctcgagtc
     8821 gacagagcgc acttgaatct cttcgcgcgt cagctcgaac gggcgagcgc cttgccaacc
     8881 ctccgagcca tcacgatgga gccatacggt gaccttggac cccggcttgc cacgcaggcg
     8941 attgaccgcg tcatcgagcg gcatgttgag cgtggactcg ttgttgatct tggtgatccg
     9001 atcgaagcgc ttgagccccg cgcgcccggc cggagtgccc ggcatcggtc gcatcaccgt
     9061 gagctgctga tcgcgaatcg aaatgacgat gccgaggcca ccgaagtgac ccgaggtcga
     9121 gagcgtcatc tctcggtacg cctccggact catgaacgtg ctgtgcggat ccaacgtgcg
     9181 gagcatgccg ttgcaggccg catactcgac ctcgcgcaag tccacgccgc tatccttgag
     9241 gttcgcctgc aaaaacgcga acacctcacg caaacgcgcc gcgacgtccc atggcccttg
     9301 cacgttgtcc acgcgaaatt cagcggactc ggtgtcgacc gtcaccttga cggtggccgc
     9361 cttgtcctcg taatgcagca tcacctgagc cacttccttt tggatttggt tcaaggcgct
     9421 gaggaacatc tggcgcggct tgacccggct cggatcgacg tacttgttcc gaatcgtgtt
     9481 gagcgtctcg ttgaccgcgg tgagctgagt caaatcgtac ggcgctttgg gtgcgcccgg
     9541 actcgtcact gcatgagccg cctcgagccc cgtccaaagg cctccccagc cgaaatggag
     9601 cgcgaagtaa gacgcaacac caaaaacccc cagcacgacg agggcgcgcc cgaggcgctc
     9661 gagaatccgc atggcccgca gtatagcgtc tagcagacgc cgattcagca gctctgctcg
     9721 ttcctagtta ttcccgagcg tcccacacct tcgagtcgat ttggcagctt cgacctgggc
     9781 cggtcgaacg tgctgattcc ccaaaccgcc gacggcagct ctcactcagc cgcagcagga
     9841 gcctggctgg gctggccggc gctagcgcgt agcccagaac ctgcggcccg gctcgcttca
     9901 cgcggcgcgc tattctgatt ctggccggtg ccggatctgg cgttactcgg tgcgtccgga
     9961 tcactcacct tgggcgaggc ctcctcctga cgaatcgagt agcgaaccag cgcccggagg
    10021 gccttgttcc gctctacgtt acccaacacc agtttcaaat cttcgtaaac ggaggggtct
    10081 acgagcagcg cccccaaggt gccacgcccc gccttcagat ccgcgacgat ctgtttcagg
    10141 tcgtggctca cgtcgttcaa gtctgcggcg aaacgatctc cgcgtgcgtc cccgaacagt
    10201 aaactacggg ccaggccatt tccctcgcgc actcctttga gcgtggtgcc gagctcctcc
    10261 gcagcttgac cgagctgcgc tactgcccgt tggccctcgt tgccatacac gacttcgtgc
    10321 gccagccctg ggccgtgctg gatctgtccg ataatctggt tgatgttgcg ggtggtcgta
    10381 gtgagctcgc cggaaacttg ctctaggtgc gcgaacgttc gggacaggcg ctccgcttcg
    10441 gcgggatccc cgaacaagcg tcccacgtaa ccttctcgcc gctcgaccgc gccgagaata
    10501 ttcgcgagcg aaccgacgga tgccttgaga tcatcgtgca gctccgggtc cgcgagcatg
    10561 cccgtcgtgc gctccaagtt acccatcacg cgctcggctt gagccgacac tgcccccaag
    10621 cgcgtgatca tgacggacat gtcgtcgccg gtttttgatt cgatgcgttc tcccggcgga
    10681 atttgcggca aactcgggtt tccgacgctg atcacgatca tcttgtcgcc gagcaaaccc
    10741 ttgccttcga tggacgagat gctgtcctta cgaatgcggc gtgcctcgcc cgcaacgatc
    10801 gacatcttga cgaagatgct ggcgtccttc gcgttttctc cgtacgaaac gtccgcgacg
    10861 gagccgatat ccacgccgcc catacggacc ggcgacccgc gccgaagccc ctgcacgtcc
    10921 ttgaagacgc tgaagtaggt ctccttactt ccgaatgccc ggcgctcttc gccgatcatg
    10981 aagatgacca tcgcgctcac cgcgagaccc gccatcacga acagtccgac ctttacttct
    11041 cgagcgaacg ccatcttgca cctgcagtgt ccctcagtct tggcgtgctg ccacccggcc
    11101 gcacggcctt ctcatccctc gcggtgacct cccgcgcgca aacgagcccc accccgtagc
    11161 tttggtcctt gcgtgaatcc gcacagggga ggccgacatg aggggcgagg aaatgaaacc
    11221 gccgaggtag ggaaagccga cgcctcccca aaccaacgac tccggggccg agcacgagaa
    11281 actcacgacg tgagcagcgt ctcgacgctc tctttgaccg gcgcccgccc ctcgacgaag
    11341 tccgaaaccc gcggatcttt ggtgctccga aattcgtcga cgttcccgac ctggatgatg
    11401 cggccgccat gcaccatcgc cacgcgatcg ctaatcgtga agacgctctt catatcgtgc
    11461 gtcacgacga tgctggtgat gtgcaacttc tcctgaagcc ccatgatcaa atggtttacg
    11521 cgcgccgtat tgatcgggtc gaggcccgtt gtgggctcgt catatagcaa tacttccggt
    11581 tgaacagcga tggcgcgcgc caatcccacg cgcttcctca taccgccaga caagtctgcc
    11641 ggtcgcatca actcgatgcc gggcaacccc accaggccta gagcccaagc cacacgctcg
    11701 tcccgctcct gtttgctcat cttttgacgg aagtgctctt ccaggccgta ggccacgttt
    11761 tctccgaccg tgagcgagtc gaagagggcc gcgccttgaa acagcatcgc gatgcgcttg
    11821 cgcacgtcgg cgagggactc ttctttcatc tgagtcactt cttggccatc gaaaagaatg
    11881 ctgccgtgat ccgctcgcag cagaccaatc agcatcttca gcatcacgct cttgcctacg
    11941 cccgatcccc caacgatcgt gaggacttcg cccttggcaa tctccaggtt caatcctcga
    12001 tagatcacct tggggccgaa ggccttacgc acgtcctgaa agcgaatcag tggctcgggc
    12061 atacggggcg acacactagc attgctcggc ggggatttcg cgcggacgac gcggtcatga
    12121 acgcgagaga ttgcgggcga gggactagag agtcggaatc cagcttcaga gcacgctttc
    12181 gacgtgcagc tccaaggcat ccccgccgcg atagcggtcg cgccgcaggc ggccacacag
    12241 cagcgcggtc gcaccgagcc gctccgagag gtcgccgagc cttggtccaa aaccggagag
    12301 cttctgcccc gaagacaact cgagctccag tttcaggtgg ccacctttga cagctcgggc
    12361 tgcgagcacc cgagtttcca ccaacagtct cggagtgggg ttatccctgc cacacggttc
    12421 gagcatggcg aaatcgcgga gcaccctgtc tggagagtct tgcaaatcca gccacacgct
    12481 ggccgcatct tccgcgggct cgaccgggcg caccgccgca accgcggctt gagcacgcgt
    12541 cgcggcctcg aacgcctctc tgaactccgg cagcttctcg gaccgtagct ccaaacccgc
    12601 cgccgcttga tggccgccga agcgctcgag cagaggcgag caagcttgca gcaaatcatg
    12661 cagccgagcc tgcgctggac cgcgcaccga ccccctcgcg cgcccacctt cgatcgcgca
    12721 caccactact ggtcgagcga agctatctgc caagcgccca gcgacgatcc cgacaatgcc
    12781 atgattccaa cccgcgcgac cgagcacgag tgacgagcgc tccgcatagc cttcacgggc
    12841 gatctcgtcc agcgcttcgt cctgcagctt ctcttgttgt gctttgcgct ctgcggaggc
    12901 ctgctccaga cgagccgcca aagcgtatgc ttcgctttcc gagcgggaca acagcaaagc
    12961 cagcgcgacg tccggcgcgc caagcctacc aggggcattc aaacgcggag cgatcgagaa
    13021 ggcgatatcc tctgccgtga gggcgccgcc accgccgatg cgagcttcgc tcagcagcgc
    13081 cttgagcccg ggccgctccg gcgccgcgag cacgtgaagc cctgcgcgaa cgagtgctcg
    13141 attgtcccca tcgagtggtg ccacgtcggc gaccgttcca atcgccacga ggtcgagcca
    13201 cgccttcaat tcgagcttct tgtcgagcgc acgccgaacg gctccaccca gcgaaagcac
    13261 tagcccgcac gacgccaggc ccttgtacgc gaagccgcag tctttacggt gcgggttgag
    13321 gaacgcgagc gcgggcagcg gagcttccgg caccaagtgg tgatcgacga cgatcgcctg
    13381 cactccggag cgggacagct cctctagggc aacatgatcc gaggagccac agtcgcacgt
    13441 cacgagcaaa cccggacgtt cgcgctggat tcgctggacc gcggagcgag ataggccgta
    13501 accgccgtcg aatcggctcg ccaacagggg cgtcacatcc cccccgagca tgcgcaatat
    13561 ctcggtcaga attgcggtcg cggtcatgcc gtcgcagtcg tagtccccga atacgactat
    13621 cttctcgcga gctcttaccg cgcgcgccaa gcgctgcgcg gcaacgtcgc gatccgccat
    13681 gccgtccggc agcgtcaagc cggacagctt gggattcaag aagtcccgcg tcttgcccga
    13741 tgcgtcgaag ccgcgctgcc ggagccacgc cgcgaacgta tatgaaaccc ctagttccgc
    13801 ggcgatgcgg ctgacttcgc ccgatggccc ggtggggcgc cgcgtaacct gaacaacact
    13861 cggagcgctc gacagcactt tgcacttcga gcactcccgc ttccataagt caactgccgg
    13921 agtggggcgc cccccgccgc tcagtcgcgc tgagcggcgc ggccgctggt attcaactga
    13981 gcggcgcggc cgctagtatt caactgagcg gcgcggccgc tagtgttcaa ctgagcggcg
    14041 cggccgctag ttcaataatc cgctctcgac actcggcgga tcgattggcc gtgccacgaa
    14101 aatccagcgt gggcgaccgt cagggcccac cacgcgcaga tgctcttcgg gcgttgctcg
    14161 tagccgaacc tgcggatcct cctccgcgtt gtcgatctcg atcaacgaca ttccgcccag
    14221 aacctgtaag gcccgcaaca cctccgcgcg cgggcccagc tgcatgagcc tagacagcgc
    14281 gagctgccgg gaatccgtga atgcgtcgag cagcgactct gcgcgccgat acactgcttg
    14341 ctgcgccgga ctcaaggttc cccacagctc accgggcagg gtcagaggca acggacgcga
    14401 ggcactcgga acagctgcgc taagtcggtg catgcgctta acagtaaaca cgcggccggc
    14461 cctgtcaatc gcgcgaccga ccggacgctt ggtcttgaaa cgtggggtat acagagctcg
    14521 accgtcggcg ttgccgcgcc tcacacggct gcggttgccc ctttgtccac cgacgccccg
    14581 cctcgcgatg agccaatgcg ctggaagagc acgcgatcta gccattccgt gaggggcaga
    14641 gccacgtaga tggaggagta ggtgccgagc accatgccca ccgtaagggc cagtgcgaac
    14701 tccttcaaag tccccgttcc ccatacgaaa aatgcgatca agctgatctg caccgtggcg
    14761 ttggtgagca gtgtccgccc caacatctct gacgtgctga gattgatcag gtgtctgaac
    14821 gacgccccgc gtagcttgcc caggttctcg cgcacgcggt cgtagatgac caccgtgtcg
    14881 ttgacggaga agccgataat cgtgagcacc gaagccacgg tggtcaggtt gatttcgcgc
    14941 cccaacaaga tcaggacgcc caccatgccc accgcgtcgt gaatgagggc gacgacaccg
    15001 ccgggcgcga atcgcagatc gaaacgaaac gcgatgtacg ccatgatgaa cacgatcgag
    15061 atcgcgacgc tcttgaccgc actgtcccgg agctgcgctc ccgcgcgcgg cccgatccac
    15121 tctgagcgca gtggttgatc cggagctttg gcgccgagcg cacgcttgag gcccgccacg
    15181 agctggtcgc ccttgctcat cagctggatc tcgacctttt gatcccgggc gttctgaatg
    15241 aacgggttgt tgccgcctgg acgcagccgg atgcccttca ctgccgagac gtgctcccga
    15301 acccaatcca ggtccggggc ctctcggaaa cgcgccgtga tcttgtcgcc accggggctg
    15361 aatttgactt cagtggcacg cttgtcggcg ggacaagccg cttcgggcaa acccggagtc
    15421 agacacaacg ccttctccac ctcggcttga gtctcggcgc tcagcgtgga gacctcttgc
    15481 acgcgaatca ggtagtggtt ctgctgcttc ccgtcctcga cgcggacgac gtccggagtc
    15541 gagaagccgg cacgctgtac ggcatctcgc acctcacccg gacccacggg agcatggaag
    15601 gctacctcga tttcggtgcc acccttgaag tccgtgccga acctcggctc gattcctggg
    15661 accttgccgg ttaccaaaac cagcgagaaa accgtcaaga acaagctcag cgctatgaaa
    15721 tagcgccgaa cgctcatgaa gtcgtaaacc ttgtggctct tgaacagatg catggctcag
    15781 ccggtcgctc tcgctagaag ttgactaccc catgtccaag cggccgtgcc gtccgagcat
    15841 gcggatccag gcctcgaaga aaagtcgcgt caccaccaca ccggtgaaga cgttgcacac
    15901 gatgccgacg atcagcgtca cggcgaaccc cttgagaggc cccgtgccgt actgcgctag
    15961 caccacgccg gcaatgaccg tcgtgatgtg gccgtccacg atcgcgctga acgcgcgtcg
    16021 atacccgatc tctaccgctg cgcgcgcgct ctttccgagc gccatttctt cacggattcg
    16081 ctcgttgatg agcacgttgc tatccaccga cataccgacg gtgagggaca aacccgcgat
    16141 accgggcagc gtcatggcgg ccccaaacat gcacaggatc gcgagctgca ggaacaggtt
    16201 cacgagcacg gcgaagtcgg cgatgagccc agcccgctga tagtaaatca gcatgaacat
    16261 gagcacgagt acgatgccgc ccagtgctcc ctgcacgccg agctcgattg catcgcgacc
    16321 caacgtggga ccgatccgct gctcgttgga gggcgagatg ggcgctggca acgcaccgga
    16381 acgcaacacg agctcgagct tgcgtgcgtc acgcagctga atttccgggt cgctcgagcc
    16441 gagcgtgatt tgagcatgcc ccccgggaat gcgcgtctga atgaccggcg cgctctccac
    16501 gcggttgtcc aaaataatcg cgaagcgacg cttgatgttg ttcgcggtga tgcgctcgaa
    16561 gatcttcccg ccttggtccg tgaacgtgag ggacacgtac caactgccca tgctggtctg
    16621 cgactggtcg ggcacggccg ccgcgtcacg gatcatgtca ccggtgactt cggcgcggct
    16681 cttgaggagg taagagcgga acccgttctc ttcctgcttt ccagtcagct cgtctaccag
    16741 gcggtattcg atctcgtagc ccacctcgcg atccggaggc acgggcaggg tcgccaccca
    16801 ctcgcggaag cgctgcaggg tttgttggct cgtctctttg gggagcttct tcagaaacgc
    16861 gaacgtgttt tggcgcgtct ggacgtcacc ctgcgcgttc ttaccgaccg agacgttctc
    16921 ggaatagaac tcgagccctt cgggcaggct ctcttccttc gcggtagacc gcacctgacc
    16981 gaagaagtcg gtgtcgtcgt cgagcaactt gaactcgagg cgcgcggtct ggctgatgat
    17041 ctcgcgaatg ttcgcgaagc tcttctcgtc cgagccgggg acctcgatga tgatgtcttc
    17101 gtcccgcgtg gagatcgcag cttcgcgcaa gccgagctcg tctacgcgac ggtgaatgat
    17161 ctcgcgagcc tgaccgaccg cgcgctcgcg gatttgagtc tcgacggccg agcggatctg
    17221 gaactcgtag gtgcgcggat cttggcgaac gtagctgagc tcgctgcgga agcgctctag
    17281 gaaccgatcg tcgatcttac cgggatcgaa gccttccttc agcttcatcc ggatcatgtt
    17341 ggcgggccgg cgaggcgcct cgagctcgac cttctcacgc agcttctgga agatctcgtc
    17401 ggacggcagc tcgtcgccct tgtgcaagcc gagcaacttc gacagctcga gccgcatgtc
    17461 ctcgtagtag tggtcgcgct tgtccttgat cgcttcgtct acgtccaccg tgtagacgag
    17521 acgcaagcca cccctcaggt ccaagcccgc gacgagccga aagtctacct tctctttcac
    17581 atacgccggg cacgggagct tgccgcccgt ggactccgag atcgtcggcc acaacacgag
    17641 aaaggccaag ccgcagatgc cgatggcgat gccagcgcgc aaacgccaac ccgcgtccac
    17701 cagctcggtc gtcatgaaca ggagccagaa gccaatcaag gccagggcga ccatggccca
    17761 gaagccgtcg acccaggctg cgcccgcggc tccgccgagc atgagcgtgg cgccgaaggc
    17821 gagcacacga cgtggacgac tgacggcgcc gagcagcaag ctgagcgccg cgcaggctcc
    17881 gaatacgatg gtcagaataa cgttgggcgc cacagtctta ctccgcctcg ttccttgagt
    17941 gcttacttct tctcggcggc ggcttgcgtt tcgacgttgt ccgcccccaa caccgacgac
    18001 ttgagcactt ccactttcac acccggcgcg atctcgagct tcgcatagcg atccgtcagc
    18061 tccacgagcc gacccacgag gccaccctga atgagcacgc gatcgccttt cttcagggac
    18121 gcgatcgcgg cggcttgctt cttctgttgc gagcggctgc tgaacaccag caacaacacc
    18181 atggggacca ccatgatgag gagcggcaag ataccgccga gcgggctcgg cggcggggcc
    18241 gcgcctgccg gcgccgaagg agccggttgc gaaacactcg gttcacgccc cggagcaggt
    18301 ggaacctgct ggtaggcaga gagcaagaac gcactgctct ttgcgccgcc aacgccatgc
    18361 gagacagggc ccgcgggcgc atgtgccttt gaatgaatag caacctcgga ggtcacgacg
    18421 ccgggcttgg aagcaagatg gacttccgag acgacgactt ccgaagcgac agaatgcgac
    18481 ccgttgccgg gcgcagatgc ggcgagcgac acgcgcaggg ctccttgaca ggcccaagga
    18541 cccgtcgttt aagggtcggg gctataccgg gaaaccacgc ttcgtgcaac atgcccatcc
    18601 tgccggggtc caaatgacgg cgcttggaga agccctgcgt acggctcggc agaacaccct
    18661 cgagggaagg ctgttgcttc cgctcatcta acctcggccc tcagagcggt cgcgccaagc
    18721 cctccacgcg agcccaatcg gcccgtcact tcgaatgaca acggttaacg agaaaaacgg
    18781 cgcccattcc aaccgctgct tgacagggtc ctaggtgagc tgtaatgaag ccggcctgac
    18841 atccccaccc gggaagatct ccggcggatt ctccgccgac atgaaggcgc gtagctcagt
    18901 ggtagagcac taccttgaca cggtaggggt cgttggttca atcccaatcg cgcctacaat
    18961 ccgtgtgtca gccgtgcttt gcgcggcctt ggatcacacg attggggttc ctgcggcgca
    19021 tcgcgcgcag atgacttgta cgttcgagcg ggcactaggc tccagcgtgg attgcgacga
    19081 tgtcgcagta tgcctcgctt cgaagtctga gcgagtggga ctcgccagga cggccagtcg
    19141 agtcatgctc gcgttccgcg tcggacgtcg tgcgggcact agaagcagcg tcctcgggag
    19201 aggcgctcac cgtcccccgc tcgggaagag ccgcgccgct tcgacaatca aagtgcgccc
    19261 cagcgaaaca cgcacagcgc cacgcggcgc attttgccgg ccccggaact cgatcatggg
    19321 cctatgagcg accagctggc gctacgccat tgcgtctggc gcgttcccgc gctggtatgg
    19381 cgcatgctcg gcaggagcgt gctcgggctc ggagcgtcca gtgcggacga caccggatcg
    19441 agcgcgcggg tccgctccga tctcgcgatc ggatcgaatc ctttctgcaa ccggcgttcg
    19501 tttagtcccg agcggggctc ggcgcgccgg aaatcagaac ccgaacgtct agacaaagca
    19561 gcgcatcgag aagtcgagaa acatcgaagg gagcacgtga accgtacatg gcattgagtg
    19621 gtggaggagc cggaggtcgg cgtttcagcc gcgataaccg cggaccccag atccgcgtaa
    19681 accacagaat acgcgtaccc gaagtgcgcg tcgtggccga agacggcagt aacttggggg
    19741 tattgtcgac tgaacaagcg ctgcgccggg cgcaagaagt cggtctcgac ttggtcgagg
    19801 tcaatcccaa agccgtgccc cctgtctgca agattctcga tttcggtaag tacaaatacg
    19861 aagagaagaa gaagcagcgc gaggccaaga agaagcagac cgtcgttgag gtcaaagaga
    19921 tcaagatccg tcccaagacg gacgatcacg acgtcgaggt caaaatccgc gcgattcgga
    19981 aattcatcga atccggcaac aaggtgaagg tgacgtgccg gttccgcggg cgcgagatca
    20041 tgcatcccga gcgcgcacag atgcagctga atctgatttt gtcgcgcgtc gaagatatcg
    20101 cgaacgtgga gcaacggccg gtgatggaag cgcggaccat ggccctggtg gtcgcgccga
    20161 aaccggtcgt attccagcgt ttgcaaagtg atcgtgcaca gcgcgagcgc gaacgcgaac
    20221 ggcaaatagc gcaaggtcaa gtcccgaccg ccgaggaaga ggaagatttc gaggatgagg
    20281 acctcgacga cgaggacgac gacgatgacg atgaggatga ggacgaagac gaagacgaag
    20341 acgacagcgc gaaggcctga gcggaagtaa acgctccctt cgaacagact tcctcgtcct
    20401 ttcgaatccg gcagctggcg cgagttaggc tcgcgtcagc tgggccggta cttaagagat
    20461 tgccgtcgag aacagcccgg tgcgaggcgg ggccgcgaac gactcccatc ccggcgggtc
    20521 cagtcaacgc cgaaacgcgc ccgcgaagta ggcgaccagg gccaaaatca gcaggatcgc
    20581 aaagaccacc ccgacgatca ggcccgggtt ggtcttcttc cgagcgaagg gcgcctcgtc
    20641 gtcatcggac gaaggatcca ttggagccgg caccgatggc ccggtgggag gtggcgccga
    20701 caccccggcg ggcggagccg atgaagcatt gggaggtgaa ctgtgcgggg gagccgatgg
    20761 agagtgcgcc gaaatcggat catcctccaa ggcggcgaga ttacccacct cgagcggctc
    20821 gggcgtgggg ttctcggctt tgctcaagcc gagatcatcc atccagtttt gttgtgcgaa
    20881 ggtgccgagg ctcaagggca tcgcacccac catcgagacc tgggacttct tgtcgccgcc
    20941 gtcgatggat gtgaactcga acagcgcctc gtcgatgagc gatccgatga ttgagccacg
    21001 accgactggg cccgcggctt tttggcgctt cttctcgaga cgctcgcgcc agatcggcag
    21061 caccagctgt gagatatcga agctcgaaac ctcgcgcccg aggtgaaaga gcaacttatt
    21121 caagtcgcgc cccagctccc gcgcactgga gtaacgcgcg ctcgggtccc ccgcgagcga
    21181 gcgcatcaac acgtcttcga gttctcgcgt gacccgcgga ttgtacttgc gaaccgacgg
    21241 cacgcgcgct tgctggacca tgcgcaccgt ctcgagatcc gactcgccca agaacagccg
    21301 ctgccccgcg aggagttccc aaagaatgat cccgaccgcg aaaatgtccg tgcgcgcgtc
    21361 gacgggtaag ccctgcgcgg cctcgggcga gagatagctg gacttgcctt tgatgattcc
    21421 aggttcgctg tgctcgagct ggctattcgc tttagccagc ccgaagtcca cgatcttgac
    21481 ctcgccgtgc cgggtgatga gcacgtttgg cggagacatg tcgcgatgca cgatcttcaa
    21541 gctctcgccc ttcgaatcgc tgagctcgtg cgcgtaggcc aggccttcgc aaatgcgaac
    21601 gcaaatcaag caggcttcct cgatgggaat gggctgcccg atgcgccgcc ggtattcgat
    21661 gacggccttg agatcggagc cgtccacgaa ttccatgacg atgaaatacg tgttgtcacc
    21721 gacgccgatg tcgaagacct gaacgcagtt cgaatgagag aggtgcgcag agagccgcgc
    21781 ctcgtccagg aacatcccga tgaactgctt cttctctgcc aagtgcggca gaacgcgctt
    21841 gatcgctacg gtcttcttga agccctccaa gccggcactt tcggcgcgaa acacctcggc
    21901 catgccaccg gacgctattt tctcgatcac tcggtagcgc tgctgctgct gggccattcg
    21961 gggctttccg ttcgtggagc tgctctcggg ttctggtcct gagccctgcg ttcccggagc
    22021 atcgccattg tgccgagcaa caaccggttt cgtccacctg tcagcgcaga ttaccggcca
    22081 ctctgtcagc cggccgagat ctttagagcc tggttgagct cgcccaggag agcggcgcat
    22141 ggtgccgcga gcaggcttga ttcgagtcga aaagcgcgag ataagccgtg cccatgagcg
    22201 ctcatgacag tttagcgggc ggcgcccacg gccggcacga agggcatgat catcctggcg
    22261 ccgcgcacgc gcacggcgcc cacggccatg atcacgccgg tcacgatgat gaccacgggg
    22321 gtcacgacga tcatcacgtc gagccgcccc ctcccgaacc ggaatcgccg gcgtggcttc
    22381 cgctgttggg tggcgggctg ttcattttgg gcctcgtagt ttacttgctc atgcaatcga
    22441 atccgtcggc gccctcgccc gctggggcaa cgggcgaaaa acccgtcgcc ggcgcggccg
    22501 cggccaaggg tgctcccgat gccgccccgc cgagcggtgg cgttccgcgt ccagctatgg
    22561 cggaccgtcc aaaccgtccg atgccggctc aagcgcccgc cggacactga gcgcgaacca
    22621 aaaaaggcgc agggacgcgc gttttcttct cgccaatcga gaaagaccgt ggcaggttcc
    22681 gcccgtcttt tcccggggcc gtagctcagc tgggagagcg catgactggc agtcatgagg
    22741 tcaggggttc gatccccctc ggctccacaa cacaagtacc tgaagcctca cggctattcc
    22801 gacgaagccg aggggaactc gcgtaacatc ctgtcgcgtg aacgaacgcg gcgatggcga
    22861 ggtcagagtg ccggctttgc gcgtgcggag
//
LOCUS       DASTKU010000686         5732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_577214, whole genome shotgun sequence.
ACCESSION   DASTKU010000686 DASTKU010000000
VERSION     DASTKU010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577214"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFQ61_23910"
     CDS             <1..690
                     /locus_tag="VFQ61_23910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957572.1"
                     /translation="GASSGGSDAQGAGGRASGGRSSTPLQGGANVGGASASNGGSAAA
                     GSASGNTAQPSQGGSSGMTNTEPEPPAALQICKRLDNVTMLSANVTKQFMGRILDDCA
                     IGLLYEEYFSAPSLLANRILQWNLPLWGCTEAATSFDLAPDSAQLSRGDATRLIEHYL
                     ASAKPILDLGSTEQRDLKGMLEYLANKRIVDQSDQPSRPCDLVGAGGNGSDEGGSGNM
                     ESGEGGAPGTP"
     gene            687..1751
                     /locus_tag="VFQ61_23915"
     CDS             687..1751
                     /locus_tag="VFQ61_23915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957573.1"
                     /translation="MRSAKLKLVLVFAGMALTQAELCEKAYAQMASSLSPSGAEVNLA
                     ETLYRQGRELMAAGKYADAAAKFAESYRLDPATGTLLNLATCQEQQGKLASAWLTFSE
                     AARAARREQRDDRVRFAEQHVSALEPRLPRLTIQVSVPSADLQVRLNGIAIGPAAYGV
                     PTPIDPGVHRIEATGPDYKPWRQDVRIEHEAESKTVVVPVLEAAPEAQPKPAARMNDA
                     NRAQPSALPPAVPTSPVVQRPVPVAAYVSGAVCVLTTTGALVTGALYLNQKDEYEKQL
                     SDGDSRSKAHALGVANLALWGAAGVSAALTAYLYFARPERTQATAVSRGQNAGVRWVP
                     WAATQEQRLVAAGLNLGSEF"
     gene            1777..3414
                     /locus_tag="VFQ61_23920"
     CDS             1777..3414
                     /locus_tag="VFQ61_23920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957574.1"
                     /translation="MTGPSRGLTHFGQIGAAALLASGCLLTNPLPDTGAGGSSGQMNS
                     SGSGGSASTGRCTSHKQCTDDHAGDPYICRASDGQCVPLVSTACPLPSNLQYATDPNA
                     IFVGAFTDLNKSELENSSAVYAYRLALDEFNNLGGLEGGPNGARRPLVAVMCYDDPEY
                     IESAMQFLTEELQIRAIVAGFRQPKDLVTSFEKYATGKVFFLSPYGGSSDLRQLNDNG
                     LVWSLLGSPGDFAEIYAALLERLERFVRKDRSFEASRELKVVTFQTQDSFSSELANAV
                     SPGLRFNQRSLDANLDAGTYLPLSFDAATEGQPDLDAKVKSILDFRPDVVLSFAGPAF
                     SGGRQRGVVGQLQTQWGSDTPRPFYVLSPLNDGSLESDDVAGFIESEALIHPEAQPYR
                     RFLGIGSGLSSDPTLRNEFNVRLATHYARAPRGTEQFYDAMYLLGYAIYGAGSVSNVQ
                     GPDIARGMKRLLNGPSFDIGPKRMGQVISVLKVPEGELSLKGTLGPPNFDVASGMRID
                     PGSVFCYDENAVLERYALTYDLDTHQLSGTFPCFDDF"
     gene            3396..4655
                     /locus_tag="VFQ61_23925"
     CDS             3396..4655
                     /locus_tag="VFQ61_23925"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9957575.1"
                     /translation="MLRRLLSAACRSGVAAPSRSWSGTGPRCRSIFCALSLALSSCGS
                     EAVDTPAPDAPSPSAATKVQPIIAGEPTEPGQDDAILMLLLHHEDGALAECSATLVAK
                     NLVLTTRHCVTYMNFEAFACTVHGEVIENPTGGGTYGAEARPDQLTFHTGQPLRTEPI
                     AYGRAIVSSSSTTICKNDIAFVILDRDLELPRIPIRMQGNVRRGELVDIVGYGLGEDM
                     DPEFSRRLRLRKEGLAILGVGPLRNDQDVTTVAPRVFTLGGPGPCRADSGGPALSSET
                     GAVIGTYSLSLRADCLADDITHHYTNVAGFTALAEQAFEAAGYPPLREDEREPSACTS
                     GNACSPTPTEGQSTNSGCALGRRGPQRRLTGEQPSRNSSSLPWLGVFGLTLGVRARRM
                     RQRRSLKISVSPAPGLALAEAAGRDDA"
     gene            complement(4574..>5732)
                     /locus_tag="VFQ61_23930"
     CDS             complement(4574..>5732)
                     /locus_tag="VFQ61_23930"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957576.1"
                     /translation="AVDYVLQACEALAEAHSYGIIHRDLKPANLFLTRRPDGSALIKI
                     LDFGISKDLLADMEAPASLTASQSLIGSPHYMSPEQVRHPKLVDSRSDVWALGVILHE
                     LIVGERPFAGDTPMSVLAAVVSDAPVKLRSLRREAPAELENVVLRCLEKDPARRFANV
                     AELARALEPWATPASRPLVARIEGIVRTASGSTPPELRPISSSPRFETSETLPSPRYV
                     NSTLQTGTLPPSTPAPAPRRALWLWGAAALGAVGGVIYVLRSSHSTRPTATTVTTATL
                     VGASDSASSLASNAPHAEEPRVELAPPSGASSGVIAPSATVTSEPAARVVSRPGGHKG
                     RPRATGAPASSGAPVNSSSPAGPSAQASSRPAASASAKPGAGDTDILSERR"
BASE COUNT         1091 a         1814 c         1829 g          998 t
ORIGIN      
        1 ggagcatcga gtggtggtag cgacgctcaa ggcgcgggag gccgggcgag cggcggccgc
       61 agctcgaccc cgctccaagg aggggcgaac gtcggggggg cctcggcgag caacggcggt
      121 agtgctgcag ccggaagtgc gtctgggaat acagcgcagc caagccaagg cggctcttcc
      181 ggcatgacca acacggaacc cgagccaccg gccgcacttc agatctgcaa gcgactcgac
      241 aacgtgacca tgctgtcggc gaacgttacc aaacagttca tgggtcggat cttggacgac
      301 tgtgcgattg gcctactcta cgaggaatac ttctcggcgc cgagtctgct cgcgaatcgc
      361 atcttgcaat ggaatctacc gctgtggggc tgtaccgaag cggccacgtc tttcgatttg
      421 gctccggata gcgcacagct gagccggggc gatgcgactc ggctgatcga gcactattta
      481 gcttcggcca aacctatttt ggacttgggc agcaccgagc aacgtgattt gaagggcatg
      541 ctcgagtatc tggcaaacaa gcgcatcgtg gatcaatccg accaacccag ccgcccctgc
      601 gacctcgttg gcgccggagg taacggctcc gacgagggcg gcagcggaaa catggaatcg
      661 ggcgagggcg gagcgccggg cacgccatga gatcggcaaa gctcaaactc gtcctggtct
      721 tcgccggaat ggcgctgacg caagccgagc tgtgcgagaa ggcttacgcc caaatggcgt
      781 cctcgctctc cccgagtgga gccgaggtca atttggccga aacgctgtat cgccaagggc
      841 gcgagctgat ggccgcgggc aagtatgccg acgctgcggc gaagttcgcg gagagctatc
      901 gcctcgatcc ggcaaccggc actctgctca acctcgcgac ctgtcaggag cagcagggca
      961 agttggccag cgcctggctc accttcagtg aggcggcccg cgcggcgcgg cgcgaacagc
     1021 gcgatgaccg agttcgtttt gcggaacaac atgtcagcgc cttggaacct cgcctgccgc
     1081 gcctcacgat ccaagtcagc gttccgagcg cggatctgca ggtgcgtttg aacggcatcg
     1141 ccatcgggcc ggccgcgtac ggagtgccca cacccatcga tcctggcgta catcgaatcg
     1201 aagccaccgg ccccgactac aagccgtgga ggcaagacgt tcgaatcgaa catgaggcag
     1261 agtccaagac ggtcgtggtt ccagtgctcg aagcagcgcc cgaagcccag cccaaaccgg
     1321 cggcgcgcat gaacgatgca aaccgggctc aaccatctgc actgccgccg gccgtgccca
     1381 cttcgcccgt ggttcagagg ccggtgccgg tcgctgcgta tgtcagcggc gcagtctgcg
     1441 tgctgactac taccggagcg ctggtcacgg gcgcgctgta tctgaatcag aaagacgagt
     1501 acgaaaagca actgagtgat ggtgattcac gctctaaagc ccacgcactc ggagtcgcga
     1561 acctggcgct ctggggcgcc gcgggcgtgt cagccgcact tacggcgtat ctttacttcg
     1621 cgcgcccgga gcggacacaa gctaccgctg tctcgcgggg ccagaacgcg ggcgtgcgct
     1681 gggtgccttg ggccgccact caagaacagc ggctcgtggc ggccggattg aacctaggat
     1741 cggagtttta ggggcgagat gtcacgctcg attcagatga ctgggccctc acgcggattg
     1801 acccatttcg gccagattgg cgcggcggcc ttgttggcct cgggctgcct gttgaccaat
     1861 ccgttgcctg acaccggcgc aggtggaagc tcggggcaga tgaacagcag cggaagcggc
     1921 ggcagcgcga gcactgggcg ttgcaccagt cacaagcagt gtactgatga tcacgccggt
     1981 gatccgtata tttgccgcgc ctcggatggg caatgtgtgc ctctcgtgag caccgcttgc
     2041 cctttacctt cgaatctaca gtacgccacg gatcccaacg cgatcttcgt aggcgcgttc
     2101 acggatctga acaaatcgga gctcgaaaac agcagcgctg tttacgctta tcggctcgcc
     2161 ctcgacgagt tcaacaatct cggcggcttg gaaggcgggc ccaacggcgc gcgccggccg
     2221 ctggtcgcgg tgatgtgtta cgacgatccg gagtacatcg aatctgccat gcaattcctc
     2281 accgaggagc tgcagatcag agctatcgtg gccggctttc gccaaccgaa agacctagtc
     2341 acctccttcg agaagtatgc gacgggaaaa gtgttcttcc tcagtccgta tggagggagc
     2401 agtgacctcc ggcaactgaa cgacaatggg ttggtttgga gtctgctcgg ctccccggga
     2461 gacttcgcgg agatctacgc ggccctgctc gagcgcctgg agagattcgt ccgaaaagat
     2521 cgctcattcg aggcttcgcg cgaactgaag gtcgttacgt ttcaaacgca ggatagtttc
     2581 agctccgaac tcgcgaatgc cgtgagccct ggcttgcgct tcaaccagcg atccttggat
     2641 gcgaacttgg atgcgggcac ctacctgcct ctgtcattcg acgcggccac cgaggggcaa
     2701 cccgatctgg acgccaaggt gaagagcatc ttggactttc gccccgacgt cgtgctttct
     2761 tttgcgggcc cggccttcag cggaggccgc caaagaggtg tagtcgggca gctgcagacg
     2821 caatggggct ccgacacgcc ccggccgttc tacgtactgt ccccgttgaa cgacggctca
     2881 ctcgagagcg acgatgtggc cggcttcatc gagagcgaag ccttgattca ccccgaagcg
     2941 cagccctacc ggcgctttct gggcatcggc tcgggcctct cctcggatcc caccctacgc
     3001 aacgagttca atgttcggtt ggccacccac tatgccagag ctccgcgtgg cacagagcaa
     3061 ttctacgacg ccatgtatct gttgggttat gcgatctacg gcgccggctc ggtcagcaat
     3121 gtgcagggcc ccgacatcgc gcgcggcatg aagcgtctgt tgaacgggcc atcattcgat
     3181 atcgggccca agcgaatggg ccaggtcatc tccgttctca aggttccgga gggcgagttg
     3241 agtttgaagg gcacgctcgg gccacccaac ttcgacgtcg cttctggcat gcgtatcgat
     3301 ccgggtagcg tgttttgcta cgacgaaaac gcggtgctcg agcgctacgc actgacctac
     3361 gacttggaca cgcaccagct gtccggcaca ttcccatgct tcgacgactt ctgagcgcgg
     3421 cttgccggtc gggcgttgcc gccccgtcga gatcttggtc gggaaccggg ccgcgatgcc
     3481 gctcgatttt ttgtgccctc tcgctggcac tgtcgagctg cggttcggaa gctgtcgata
     3541 cgccggcccc ggacgcgccg tccccttcgg cggcaactaa agtccaaccc atcatcgccg
     3601 gtgagccgac agaaccgggg caggatgacg cgatcctgat gctcttgttg catcacgagg
     3661 atggcgcact cgcggagtgc agcgcgaccc tggtcgccaa gaatctggtg ctcacgacgc
     3721 gccactgcgt cacgtacatg aacttcgagg cgttcgcctg cacggtgcac ggggaggtca
     3781 tcgagaaccc gaccggaggc ggaacctacg gcgcggaagc gcgccccgat cagctgacat
     3841 tccatacggg acaaccgctt cgaaccgaac ccattgccta cgggcgcgcc atagtttcga
     3901 gcagctccac gaccatctgc aaaaacgata tcgccttcgt cattctggat cgagacctcg
     3961 agttgccacg catccccatt cgcatgcaag gcaacgtgcg gcgcggcgag ctggtcgaca
     4021 tcgtaggtta cgggctcggg gaggacatgg acccggagtt ttcacgccgc ctccgattgc
     4081 gcaaagaagg gctcgcgatc ctaggagtgg gaccgctgcg aaatgaccag gacgtcacga
     4141 ccgtagcgcc gcgcgtcttt acgctaggag gccccggtcc ttgccgtgcc gatagcggcg
     4201 ggcccgcgct ctcgagcgaa accggagcgg tcatcggcac ctattctctg agcttgcgcg
     4261 ccgactgcct cgccgacgac atcactcacc attacaccaa cgtggcgggc ttcaccgccc
     4321 tcgccgaaca agcgttcgaa gcggcgggat atccaccgtt gcgcgaggac gagcgggagc
     4381 ccagtgcctg caccagcggc aacgcatgta gtcccactcc gacagaaggg cagagcacca
     4441 actcgggctg cgccttgggt cggcgtggcc ctcagcggcg ccttactggc gagcaaccga
     4501 gtaggaactc gagttcactg ccgtggctcg gcgttttcgg actgactctc ggtgtgcgcg
     4561 cgcgacgtat gcgtcagcgc cgctcactca agatatcagt atctccagca ccaggtttgg
     4621 cgctcgccga ggccgccggt cgcgacgacg cctgagcgct tggcccagcc ggggagctcg
     4681 agttcactgg agcgcccgag ctggcaggcg cacccgtcgc acgaggccgg cctttgtgac
     4741 cgcccgggcg agagacgact cgggcggcag gctcgctcgt aacggtggcg gaaggagcga
     4801 tcacgccact cgatgcgccg ctcggcggcg caagctccac gcggggttct tccgcgtgag
     4861 gagcgttcga agcgagcgaa gaagctgagt cgctcgctcc cacgagcgtc gcggtggtca
     4921 ccgtggtcgc cgtaggacgc gtggaatggg acgagcggag cacgtaaatg accccaccga
     4981 cggctccgag agcagctgcc ccccagagcc aaagcgcacg gcgtggtgcc ggcgctggcg
     5041 tcgaaggcgg gagggtgccc gtttgcagag tcgagttcac gtaacgaggc gaaggtaacg
     5101 tctccgaggt ttcgaagcgg gggctgctcg aaatgggcct caactccggc ggcgtcgagc
     5161 ccgaggcggt ccgtacgatc ccctcgatac gcgcgaccag cggccgcgat gccggcgtag
     5221 cccacggctc cagagcgcgc gcgagttccg caacgttcgc gaaacggcgc gccggatctt
     5281 tctccaggca acgcaacacg acgttctcga gctcggccgg cgcctcgcgt cgcaagctgc
     5341 gcagcttaac gggagcatcc gaaaccaccg ccgccagaac ggacatcggg gtatcgccgg
     5401 cgaagggtcg ctcgccgacg atgagctcgt gcaggatcac cccgagcgcc cacacatcgc
     5461 tgcgcgagtc caccagtttg ggatgccgca cctgctcggg tgacatgtag tgcggagacc
     5521 cgattagact ttggctcgcg gtgaggctcg ccggagcctc catgtccgcc aacaaatctt
     5581 tggaaatccc aaaatccagg atcttgatca gcgctgatcc atcgggacga cgcgtgagaa
     5641 acaggttcgc gggcttgagg tcgcgatgga tgattccata gctatgagcc tccgcgagcg
     5701 cctcacacgc ctgcagcacg taatccacag cc
//
LOCUS       DASTKU010000687         7987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_131830, whole genome shotgun sequence.
ACCESSION   DASTKU010000687 DASTKU010000000
VERSION     DASTKU010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131830"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFQ61_23935"
     CDS             <1..289
                     /locus_tag="VFQ61_23935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957577.1"
                     /translation="AGGDAVGGVSDGARVTCWRMRGLAGAVGSWVCASGGVDGGRARC
                     WARRAEEIGCLIGLIGGDESVQKNEFPCCVPGLFLARWEGWEAQDECFGVA"
     gene            420..1043
                     /locus_tag="VFQ61_23940"
     CDS             420..1043
                     /locus_tag="VFQ61_23940"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9957578.1"
                     /translation="MNREHPRHVTLSVAIRSLRTQRVFAVVCGVIRAVNRLRGNEFRI
                     VEFSVQSNHIHLMVEADNDAALSHGMQALTIRLVRRINRLLGRRGSLFTDRFHDRSLK
                     TARAVRQVLAYIFANFRKHGTVEALRGFSLDPFSSAPFFDGFCAADFDSVFSSPVGFG
                     PSGMSGRPPPDASSTRGNVQGQSRFLRGGWKREGLLSIGYWPASGNH"
     gene            1258..2058
                     /locus_tag="VFQ61_23945"
     CDS             1258..2058
                     /locus_tag="VFQ61_23945"
                     /inference="COORDINATES: protein motif:HMM:NF015949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HET9957579.1"
                     /translation="MSGSKTSDIYEVLQRRAAGARSVTRGVKRVVLGLNWSLAEVDSI
                     GLCFTPGAQSSVGQSRTLEWPGTLTRRSPEELASWLRSADPLEATIGMCITNALVNDS
                     ENACLKSATRLGAGPNGHLAVFAHFDALLGDARVAVVGRYPGLEVLWRHRVYQCVERR
                     PFADTLPESEAERVLKGADWVFVTASALPNHSLPRLLEQARGASVVLMGPTLPWLEEW
                     RDFGVDYLAGVGVENPEELGRVVSEAGGTRIFEGAVSYRLLALGRASI"
     gene            2566..3861
                     /locus_tag="VFQ61_23950"
     CDS             2566..3861
                     /locus_tag="VFQ61_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957580.1"
                     /translation="MTSAIARCVGVSALTVLAFACSSEVGAPAAGGGVAGSPAQGGAG
                     GVVGSGGATSPVAGSNSGTASGGTTATTSPATGGATTGTPGGAGSSGASGGSGGIAQG
                     GGIAQGGAAQGGAAQGGASGGSVQGGSTATAGAGSGGAAGSTGTGGGTAGETAKFSFF
                     VTSQKVIVELAKTINGGEKGFGGDLRFGETGAGAGLRGADKICATIAEKSMPGAGRKV
                     WRAFLSAVDGGDGKQVNAIDRVGTGPWYDRRGRLFAMTKTDLIATRPVGANSAIANDF
                     PNEDGVPNHAPDGATQVDNHDMLTGSNDKGELYGATATCKDWTSVAPSADGRPRVGHS
                     WPRSGTPIGGGAFPGGGTPAAGAGGSGGGMMGGGFAVGGMSGGGFGGINGDHWISALD
                     EAGCAPGINTVEMGPPKVSDGTVGSGGGYGGFYCFALTP"
     gene            3899..5014
                     /locus_tag="VFQ61_23955"
     CDS             3899..5014
                     /locus_tag="VFQ61_23955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957581.1"
                     /translation="MSLRSSLIWASVSCWLGSATWACSSADSPADAGGAPPGAGGAVV
                     SGGSSSGGNSSGGGSTGGTSATPGGASSGGSTTTGGSSPTGGAATGGGAGVDVSKFWG
                     TFTLSLNAEKLGAPAFTGVLGVVYDRFNPPRTVPLKVQKELNGCQLLVPQAFFCSSCP
                     SETGVCTAEEVCTPNGKPQNLGNVKVSGLKPADFVMEPFPPGFDYQPTDSVPNPACDE
                     GAPVGVETSSFRIQGTCISQLVLEGPETISVRAGQPVAVRWKAPSKPELSKIAIHLDI
                     AHHGGKKGEINCEVPDNGSFDIPEPLVTDLVSLGLAGFPTIEVTRRSEASADKAPQVK
                     LVFASEVERNVDTGVNSCSSDTDCSGGKTCKPDKTCG"
     gene            complement(5127..5819)
                     /locus_tag="VFQ61_23960"
     CDS             complement(5127..5819)
                     /locus_tag="VFQ61_23960"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET9957582.1"
                     /translation="MSRRLSKALAGLSFASPTAYVYDPLTYARAPHEAYLARYGERTG
                     RILMLGMNPGPFGMAQTGVPFGDVRMVRDFLGIQAPVDRPTREHPARPVLGFACPKSE
                     VSGTRLWGFARERFGSAERFFEQFFVANYCPLAFLEESGKNRTPDRLLAVERTAVNQA
                     CDAALREAVELLQPSLILGVGGFAEESAKRAVGDRVRVECILHPSPANPRANSGWAKI
                     VEEQLRALGVEL"
     gene            6071..7699
                     /locus_tag="VFQ61_23965"
     CDS             6071..7699
                     /locus_tag="VFQ61_23965"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9957583.1"
                     /translation="MRAKNRVHTLPRQDPLAKVNPPKAGSSLPPSGQMLSFRAGEVVA
                     DRYELIRCIGTGAMGAVFRAHDRVLDAELAIKLIRIDRERIDADRATKRLLLEARAIA
                     RISHPGIVRVFDFGSSGEVPFIVMELLQGESLLELMSREGAMDAVRAVRALLPIADAL
                     SVAHRRGVVHRDIKPDNVFLHRDEFARVQPKLVDFGVARVQQGSGFTRPGALMGTPDY
                     MAPEQAQAEPDVDHRADIWAFGVLLYELMTLRRPFAEGAHGNYLAVLKAVVGSPPRPI
                     LDYGVGDAALWAILSRALEKARHQRFQDIREMGEQLSRWLLARGVTEDAYGGSLISNW
                     SVVGEPLSPVPELTSMTMRRGSTGELELQSASDPSWPPGMRPTPVVPQAIPQQARPSR
                     PVTGQSAAPGSMLKAASARPTAISERVAHAVASLKPPASIKSESVKSEPAPERARTRS
                     ESARSENVRPSSARPERAVASSAPSATSTVTDPPTGGRLAGKLRLRPTPSEWMAVVIA
                     ILLASLLAVVSVWFGALGAEPEPQPHSDTVGAAR"
BASE COUNT         1388 a         2246 c         2891 g         1462 t
ORIGIN      
        1 tgcggggggc gacgcggtag gtggcgtgag tgacggagcg cgcgttacgt gttggcgcat
       61 gcgcggtctc gcgggggcgg tggggtcatg ggtctgcgcg agtgggggag ttgacggggg
      121 gcgcgctcgt tgttgggcgc gccgggctga ggagattggg tgtttgattg ggttgatcgg
      181 aggtgacgag tcggtacaaa aaaatgagtt tccctgttgc gtgccgggtc tgtttttggc
      241 aaggtgggag ggatgggagg cgcaggatga gtgctttgga gtcgcgtgag gcgcggtacg
      301 aaggagcgca agggaccgca aatgagcggg gaacggggcg ttggggcggg aagcgggctg
      361 gtgcggggcg gaagccgagc gggacgaggg caggggtttc gcatcggaag cgagaggcgg
      421 tgaatcgaga gcatccgcgg cacgtgacgt tgagtgtggc gattcggagt ttgcggacgc
      481 agcgtgtgtt cgcggtggtt tgcggggtaa tccgcgcagt gaatcgattg cgagggaacg
      541 agttcaggat cgttgagttt tcggttcaat cgaatcacat tcacctgatg gtggaggcgg
      601 acaatgacgc tgctctgagc cacgggatgc aggcgctgac gatccgcttg gtgcgaagga
      661 tcaaccgctt gttggggcga cgtgggagtc tattcacgga tcgttttcac gatcgatcgt
      721 tgaagacggc tcgcgccgtg agacaggtgc tggcatacat cttcgcgaat ttccgaaagc
      781 acgggacggt ggaagccctt cgggggtttt cactggatcc gttctcgtcg gctccgttct
      841 tcgatggatt ctgtgctgct gacttcgata gtgtgttcag ctcgccagtt gggtttggtc
      901 cgtcggggat gagcggtagg ccaccgccgg atgcatcgag cacgcgcgga aacgtgcaag
      961 gacagagtcg ctttctaaga ggaggatgga agcgggaggg gctcctttcg atcggatatt
     1021 ggccagcttc gggcaatcac tgagtcacat gtcagccttg cgtagagtct tgcgctcaag
     1081 cgacaagcgc acgggataag cgagcggggg caagcgaacg caggctaaac agccgggtga
     1141 ataaggaagc ggagcccgaa agagacgtgt tcgatcatgg ccccaggggc gggaacttgg
     1201 tggaaacttt ggggttacgt ggccaacatg atttctcacc cattgcgaga gtcacgaatg
     1261 tcaggttcga aaacgagcga tatctacgag gtcttgcagc gacgagcggc cggcgcgcga
     1321 tcggtaacga ggggggtgaa gagggtggtg ttggggttga actggtcgtt ggcggaggtg
     1381 gattcaatcg ggctttgttt cacgccgggc gcgcaatcgt cggtgggtca aagtcgaacg
     1441 ctggaatggc cagggacgct gacgcggcgt tcgccggagg aactcgcgag ttggctgagg
     1501 agcgcagatc cgctggaggc gacgatcggg atgtgcatca cgaacgcgct ggtgaacgac
     1561 tcggagaatg catgtctgaa gtcagcgact cggctcggcg cgggaccgaa tgggcacttg
     1621 gcggtgttcg ctcatttcga cgcgctgctc ggggacgcac gggtcgcggt ggtcggccgt
     1681 tatccagggc tcgaggtgct gtggcgtcac cgggtatatc aatgcgtgga acgccggccg
     1741 ttcgcggata cgctgccgga gagtgaggcg gagcgcgttt tgaagggagc ggattgggtg
     1801 ttcgtgacgg cgagcgcgct gccgaatcac tcactgccaa ggctgctcga gcaggctcgt
     1861 ggtgcttcgg tggtgttgat gggtccaacc ttgccgtggc tcgaggagtg gcgagatttt
     1921 ggcgtcgatt acttggctgg tgtcggcgtg gagaatccgg aggaactggg tcgagtggtt
     1981 tcggaagcag gtggaacacg cattttcgag ggggccgtga gctatcggct actcgcccta
     2041 ggtcgagcgt cgatctagct cgggggttag cgtctcgacg catgagtgcc atgggcgcgt
     2101 tgaagcgatc aaaagctggg agcgaaggtc gttggccggt cgctcgcgcg agcaggggac
     2161 gaatatgccc ccagtgccgc aggccgaacc aatttgcgag gtgaagctgc gattgagagc
     2221 cgcgccgaca gggtcagagc gtcatccgtt ggaaaaattg cgcgcgactg gccagctcac
     2281 ggataaaccg gaattgcctg gtttggcgca gtattgggac tacccttcgt gacgcacgcg
     2341 tgactcgata tgtcccgcga gctgagtccg gactgctgcg ggcgggttcg gatttttcga
     2401 actaggcttc taacaatcgg gcgcggccac gggtcgtcgt ggttttaggt gagaacggac
     2461 atgagaaaac aggcagcgaa ttcgtccaca cgggttaatt ctgttcgggc tattgtccgg
     2521 gcttccgagc aggccgatgc caccacggca ggcagcaagc gtcgcgtgac gagcgcgatt
     2581 gcacgctgcg tgggcgtctc tgccctgacc gttctagctt tcgcttgtag ttcagaggtg
     2641 ggtgcgcccg cggcgggcgg tggagttgcc ggttcgcccg cgcaaggcgg cgctggaggc
     2701 gtcgtaggtt cgggcggcgc aacttctccg gttgcaggta gtaactcggg tacggcgtcc
     2761 ggcgggacca cggccactac ttctcccgcg acgggtggcg cgacgaccgg caccccgggc
     2821 ggcgccggct cttcgggcgc ttcaggcggc agcggcggga ttgcacaggg cggcgggatt
     2881 gcgcaaggtg gggctgcaca gggcggcgcg gcacagggcg gcgcgagcgg tgggagcgta
     2941 cagggcggaa gtaccgcgac cgcaggcgca ggctcgggtg gtgcagctgg tagcaccggc
     3001 accggcggtg gaaccgcggg cgagaccgcg aaattcagct tcttcgtcac gagccagaag
     3061 gtcatcgtgg agttggccaa gaccatcaac ggcggcgaga agggcttcgg cggcgatttg
     3121 cggtttggag agacgggcgc gggcgcaggg ctgcgcggcg cagacaagat ctgcgcgaca
     3181 atcgcggaga agtcgatgcc cggcgcgggt cggaaggttt ggcgcgcgtt cctgagcgca
     3241 gtggacggcg gcgacgggaa gcaagtcaac gctatcgatc gggtgggcac aggtccctgg
     3301 tacgatcgac gcgggcggct gttcgcgatg accaagacgg acctgatcgc gacccggccg
     3361 gtgggcgcga actccgccat cgccaacgac ttcccgaacg aagatggagt gcccaatcac
     3421 gcaccggatg gggcgacgca ggtcgacaac cacgacatgc tcaccggcag caacgataag
     3481 ggcgagctct acggggccac ggccacctgt aaagactgga cgagcgttgc tccttctgcc
     3541 gatgggcggc cgcgcgtcgg ccactcctgg ccacgtagcg gtactccgat tggcggcggc
     3601 gcattccccg gtggtgggac gcccgcggcc ggcgcgggtg gctccggagg cggcatgatg
     3661 ggcggtgggt ttgccgtagg tggtatgtcc ggtggcggat tcggcggcat caacggcgac
     3721 cactggatct ccgcgctcga tgaggcgggc tgcgcccccg gtatcaacac cgtcgagatg
     3781 ggccctccga aggtttcgga cgggaccgtc ggatcgggcg gtggatatgg cggattctat
     3841 tgcttcgctt tgacgccgtg agtagttgcc acaggaatct catccgcttc gggtactgat
     3901 gtcacttcga tcgtctttga tttgggcttc ggtttcgtgc tggctcgggt ccgcaacctg
     3961 ggcatgttct tccgcagact cacccgccga cgcgggtggc gctcctcccg gtgccggcgg
     4021 cgctgtggtg tcgggaggaa gctcctcggg tggtaactcg agcggcggcg ggagcacggg
     4081 cggaacgagc gctacgccgg gaggggcctc gagtggaggc tctacaacca ccgggggcag
     4141 cagtcctacc ggcggtgctg ctaccggcgg cggtgccggc gtggatgtct ccaagttttg
     4201 gggcacattc acgctgtctc tgaatgcgga gaagctcggg gcgcctgctt tcacgggcgt
     4261 cctcggggtc gtctacgatc gcttcaatcc cccgcgcacg gttccgctga aggtccagaa
     4321 ggagctcaac ggttgccagc tgctggtgcc gcaagccttc ttttgtagct cgtgcccgag
     4381 cgaaaccggc gtctgtacgg ctgaggaagt gtgcacgccg aacggcaaac ctcagaatct
     4441 ggggaacgtc aaagtctcgg ggttgaagcc ggccgatttc gtgatggagc cgttcccgcc
     4501 cggcttcgat tatcaaccga ccgattcggt cccaaacccg gcgtgtgacg aaggggcgcc
     4561 ggttggtgtc gagacgagca gcttccggat tcaggggact tgcatctcgc agctcgtgct
     4621 ggaggggccc gagacgattt cggtgcgcgc gggccagccc gtggccgtgc gctggaaggc
     4681 tccgtccaag cccgagctgt ccaagatcgc gattcacctg gatatcgctc accatggcgg
     4741 caagaagggt gagatcaact gcgaggttcc ggacaacggt tcattcgata tccccgagcc
     4801 gttggtcacc gatttggttt cactcggcct cgcgggtttt ccgaccatcg aggtgacgcg
     4861 acggtcagag gcgagcgcgg acaaggctcc gcaagtgaag ctggtcttcg cctcggaggt
     4921 ggagcgtaac gtggacacgg gtgtgaactc gtgcagttcg gacaccgatt gttcgggcgg
     4981 caagacctgc aagcccgaca agacctgcgg ctgagcctgg atccgtcggg aaattcaggc
     5041 tcggtcagcc gagtcgaaga ggcacagcca agtcgaagag cagtcgaaga gcagtcgaag
     5101 agcagtcgaa gagcacagcc gagtcgctac agctcgaccc cgagcgctcg gagctgctct
     5161 tcgacgatct tggcccaccc gctgttggca cgcggattcg ccggactcgg gtgaagaatg
     5221 cattcgaccc gaacccggtc gcccacggcc cgttttgcgc tctcctcggc gaagccgccc
     5281 acgcccaaga ttaagctcgg ttgcaacaac tcgacggctt cacgtagcgc cgcgtcgcaa
     5341 gcctgattga ccgcggtccg ctcgaccgcg agtaaacgat cgggcgtgcg gttcttgccg
     5401 ctctcttcga gaaaggcgag cgggcaatag ttggcgacga agaactgctc gaagaagcgc
     5461 tcggcgctgc cgaagcgctc ccgagcgaag ccccacaaac gggtgccgct cacttcactc
     5521 ttcgggcatg cgaagcccag cacgggacgc gcgggatgct cgcgcgtagg acggtcgacg
     5581 ggcgcttgaa tgcctaggaa atcacgcacc atccgtacat ctccgaaggg aacccccgtt
     5641 tgcgccatgc caaacgggcc cgggttcata ccgagcatga ggatgcgccc ggtgcgttcc
     5701 ccataacgag ccaggtaggc ttcgtgcgga gcgcgcgcat acgtgagtgg gtcgtagaca
     5761 tacgcggtag gggaagcgaa gctgagcccg gcgagtgctt tggacaagcg gcggctcacg
     5821 gaggcgagag gtgggatgcg tgcggctggc atgcaggagc gcccctctta ctctggtttg
     5881 aaggtcgacg cgacttccgc cgttacatcc gagaaacggg ctcaaatgca cgaagtctaa
     5941 tccgatatgc gtgatcgcca ctggcgggtt ccgcccgctt cgtacgggaa acacaccctc
     6001 gctcgcgtct cgaatcgtta cacaccacag actcgacgcg tggcactgaa ggctagtttg
     6061 acacggccgt atgcgagcga agaatcgggt gcataccttg ccgcgccaag atcctctcgc
     6121 aaaggtcaat ccgccgaagg ccgggtctag tctgccaccc agtgggcaga tgctgagttt
     6181 tcgggctggt gaagtcgtag ccgatcgcta tgagctgatc cgttgtatcg gcaccggcgc
     6241 gatgggtgcg gtgttccgcg cgcacgatcg cgtgttggac gctgaactcg cgatcaagct
     6301 gattcggatc gatcgtgagc gtatagacgc cgatcgggcc accaagcggc tactgctgga
     6361 agcgcgcgcc atcgcgcgaa tcagccatcc cggtatcgta cgggtgttcg atttcggctc
     6421 ctcgggcgag gtgccgttca tcgtgatgga gctgctgcag ggagaatcac tgctcgagct
     6481 gatgagccgc gagggcgcga tggatgcggt gcgtgcggtg cgcgcgctgc tgccgatcgc
     6541 ggacgcgctc tccgtcgctc accggcgcgg ggtcgtgcac cgcgacatca agcccgacaa
     6601 tgtcttcctg caccgcgacg agttcgcgcg cgtgcagccg aaactcgtcg acttcggggt
     6661 agcgcgcgtg cagcaggggt cgggctttac gcgacccggt gcattgatgg gcacgcccga
     6721 ctacatggcg ccggagcagg ctcaggccga acctgacgtg gaccaccgcg ccgacatttg
     6781 ggcgttcggc gtgctgcttt acgagctgat gacgctgcgg cgtccgttcg cggaaggcgc
     6841 tcacggtaat tacctcgccg tgctcaaggc ggtggtgggc tcaccgcctc gccccatctt
     6901 ggactatggc gtgggtgatg ccgcgctgtg ggcgattcta tctcgagcgc tcgagaaggc
     6961 gcggcaccag cggttccaag acattcgcga gatgggcgag cagctctcgc gttggctctt
     7021 ggcgaggggc gtgaccgagg atgcttatgg tggttcgctg atttcgaact ggagcgtggt
     7081 gggtgaaccg ctgtcccccg tgcccgagtt gaccagcatg accatgcgcc gcgggagcac
     7141 cggcgagctc gagctgcaga gcgcgtccga tccatcctgg ccgccgggta tgcgacccac
     7201 cccggtagtg ccgcaagcca ttcctcagca ggcccgccct tcacgaccgg tgacggggca
     7261 gagtgcggcg cccggcagca tgctcaaggc cgcttcggca cggccgacgg ccatctcgga
     7321 gcgcgtcgcg cacgcggtgg cttcgttgaa gcctccggct tcgatcaagt ccgagtctgt
     7381 caagtccgag cctgcgcccg agcgcgcgcg cacccgctcc gagagcgccc gttccgaaaa
     7441 cgtgcgtccc agcagtgcgc gtcccgagag ggccgtcgcg tcgtcggctc cgagcgccac
     7501 gagcacggtc actgacccgc cgacgggcgg gcgcttggca gggaaattac ggctgcggcc
     7561 cactccgagc gagtggatgg cggtagtgat cgcgattctg ctcgcgtctt tgctggcggt
     7621 cgtctcggtt tggttcgggg ctctcggtgc tgaacccgag ccgcagcctc actcggatac
     7681 cgtcggcgct gcgcgctgaa cccaggcgtt ataaggctga taggcgcgag tggggcatgg
     7741 cgcgcggctg atgcacgggg tggcttcggc gcgcccttgg cgcggtccgc gccgggtcgt
     7801 ggtatatgga cgcccgaggt catacgcccc gtgcaaccag agtccgcaaa cgacctagat
     7861 ggctacaaag aagccgccct atccgcgcta gccgagctgc gggacgagaa ggcgctcgaa
     7921 ggttggtacc gcaagcattg ggctccctcc ggtacgctga accagtggcg caagcgcatc
     7981 ggcgcag
//
LOCUS       DASTKU010000688         3812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1918443, whole genome shotgun sequence.
ACCESSION   DASTKU010000688 DASTKU010000000
VERSION     DASTKU010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918443"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3413)
                     /locus_tag="VFQ61_23970"
     CDS             complement(<1..3413)
                     /locus_tag="VFQ61_23970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957584.1"
                     /translation="MTDRRTFWRAATLLIWLAVFFVASGVGVILHLNLPQSRRVLGRL
                     ATSALNQNLQGRFEVGEIEQIGPQNLIVNGFSAHDPQGRRVLIASRVALSVDLITLVR
                     KLFTDTDAPLTIAIEHAQLASVEAHWYESQDGTPSLLQTFTPRPSPAAPQSSAARPVR
                     VGVRSIALNRGYARGAPAGLPTLELEISQARGWVLATQSGAELRFDRFGAVLRGFGGA
                     DTRGVASVHVRTPGPLAVSFDGFHEAIQFGAEVRAQGGNVGIALDLPRVQPNDARALF
                     PEYPLLEQASAQLELIGKLPELKLSARVTTTSSSFNAAGPLHLGTAPRARLEVDGTAI
                     DLAALAPGLPRTRFDARALLDLKLGPKFELGLTGRTAPTVAFGAELPALELLANLDSE
                     HISGHARAREPGSPVAIDFERRDAGPWKLGIEIPRLVLERNPRLASLETRGTASLRAS
                     ATLDRGAAQGDFELGVKGLEHVDAAARELKLAGSFRGRVENLSRLELTSKLQARDARL
                     LDFPFRELNVSLSGPLSQPVLKLEANDRNGSQYQASARLAPRTESGQLIGVGVSRLEL
                     VARRGRSELTGKAEQVELRRQQLKIQNLVAQGFGGSLSGSAEVTPSVVHLDARGSGLD
                     LGSLGRFLGLPPHYVRGALAFDADCVLARDIETGTVHVRLEQASVGPLDGLTLTARAA
                     LENHRLALDADAQVASLGLGRLQAAVEVPTTVRDLRALQRDALGRIEVEVGRVDLGLL
                     GNLLAPGTSSRLEGVVGMRAVATRSRAGALPDLELTAQSLGLRVPAELTGLKNPVEGL
                     DVGLGLRMAGGTGDTEASVKLLDHRGLLASATASARYDVEGTLRQPQRVVQALLDTPI
                     LGKLVIEDRALAEWPAFLVPPRGPSGRARAELTWSGSPRSPDVAAKLRLAGLELTSDG
                     NSQPLDLCLNAAWSNAAHALGSSGQLYLTERGRETCGGTRVAHYTLGATAVLNAGTEP
                     QLDGNALLVLEAFPLTAVPSLKESGLSGLMSGRASLSRTGGAPAFSANVSLAQLGVRG
                     TSVGDGRLEVRSGQDTVGATLEIGDARSHIQGTVLAALDHSAGFPKLKRGEPVGVRLS
                     AQRANAALLAPLTHEVLADLGGELDAEVTAALVPGTDPANAAFSSQIGG"
     gene            complement(3603..>3812)
                     /locus_tag="VFQ61_23975"
     CDS             complement(3603..>3812)
                     /locus_tag="VFQ61_23975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957585.1"
                     /translation="ESDAAARAIAARMQLRFPIVVDRDGVLANKFNVEALPSVVVVDS
                     ARRVRWIGGTDMTQEGLLAAVDSAD"
BASE COUNT          637 a         1269 c         1270 g          636 t
ORIGIN      
        1 tgtccgccaa tctgactgct gaaggcagcg ttcgcgggat ccgttcctgg cacgagcgcc
       61 gcggtgacct cggcgtccag ctcgcctcca aggtccgcta atacctcgtg cgtgaggggg
      121 gcgagcaagg ctgcgttcgc ccgctgggcg gagagtcgaa cccccacggg ttctccgcgt
      181 ttgagcttgg gaaaacccgc ggagtgatcc agcgcggcga gtacggtgcc ttgaatatgg
      241 cttcgagcat cgccgatctc cagtgtggct ccgacggtgt cttggcccga tcgcacctcg
      301 agccgaccgt cccccacgga ggtgccgcgt acgccgagct gcgcgaggct gacgttcgcc
      361 gagaaggcag gcgcacctcc cgttcgtgag agcgacgccc gcccactcat cagtccgctc
      421 aacccgctct ccttcaggct ggggaccgcg gtcagcggga acgcctccag caccaacaag
      481 gcgttgccat cgagttgcgg ttcggtgccg gcgttcagca cggcggtggc cccgagcgtg
      541 tagtgggcca cccgggtacc tccgcacgtc tcgcgtccgc gctcggtgag gtaaagttga
      601 ccactcgagc cgagcgcgtg cgcggcgttg gaccaggcgg cattcaagca caggtccaga
      661 ggttgactgt tgccgtcgga agtcagctcc aagccagcta ggcgcagctt ggccgcgaca
      721 tcgggtgagc ggggagaacc cgaccaagtc aattcggcac gggcccggcc cgaagggccc
      781 cggggcggca ccaagaacgc aggccattcg gcaagcgccc gatcttcgat cacgagtttc
      841 ccgagaattg gtgtatcgag cagagcttgc acaacgcgtt gcggctgtct cagcgtgccc
      901 tccacgtcgt aacgcgccga cgcggtcgca gacgccagca gcccgcgatg gtccaggagc
      961 ttgaccgatg cctcggtgtc tccggttccc cccgccatgc ggagaccgag acccacgtcg
     1021 agtccctcga cgggattctt cagtccggtc agctcggcgg gcacccgcag ccccaaactt
     1081 tgcgcggtga gctcgagatc cggaagggcg ccggcgcgcg aacgagtggc gacggctcgc
     1141 atgccgacca cgccttcgag tcgcgagctg gttccgggcg ccagcagatt gccgagcagt
     1201 ccgagatcga cgcgtccgac ctccacctcg atccgaccca gcgcgtcccg ctgaagtgcg
     1261 cgcaggtcac gaaccgtggt cggaacctcc accgcggcct ggaggcggcc caaaccgagg
     1321 ctcgccacct gggcgtcggc gtccaaggcg agtcgatggt tttccaaggc tgcccgcgct
     1381 gtgagagtca agccgtcgag tggccccacc gaggcttgct ccaaacgcac gtgaacggtg
     1441 ccggtctcga tgtcgcgagc caaaacgcag tccgcgtcga acgccagcgc accacgaaca
     1501 tagtggggtg gtaaacccaa aaagcggccc aaacttccga gatcgaggcc gctgccgcgg
     1561 gcatccaggt gcactacgct cggcgtcact tcggcggacc cgctcaggct gcctccgaag
     1621 ccttgggcga ccaagttctg gattttgagc tgctggcggc ggagctctac ctgctcggct
     1681 ttcccggtca gttcggagcg acctcgccgc gcgacgagct ccaatcggct cactccaacc
     1741 ccgatgagct ggcccgactc cgtgcgcggc gcgaggcgcg cgctcgcctg atattggctg
     1801 ccattgcggt cgttggcctc gagcttcagc acgggttggc tgaggggacc gctgagcgat
     1861 acgttgagct ctcgaaacgg gaaatccagc agccgcgcgt cgcgcgcctg cagcttggac
     1921 gtcagctcga gccgactcag gttctcgacc cgacctcgga atgacccggc cagtttcagc
     1981 tcccgagccg cggcgtctac gtgctcgagc cctttgacgc ccagctcgaa gtccccctgg
     2041 gctgcgccgc ggtccagggt ggcggaagcg cgcaggctgg cggtgccgcg cgtctcgagg
     2101 gacgccagcc tggggttacg ctccaggacc aggcgcggaa tctcgatccc gagtttccac
     2161 ggcccggcgt ctctgcgttc gaaatcgatc gccacgggcg agccgggctc gcgtgctcgg
     2221 gcgtggccgg aaatgtgctc actatcgagg ttggccagca gctccaaggc ggggagctcc
     2281 gcaccgaatg ccaccgtcgg agccgtgcga ccggtcagcc cgagctcgaa cttgggtccg
     2341 agcttcagat cgagcagggc gcgtgcgtcg aagcgtgtcc ggggtagacc cggagctagc
     2401 gcggcgagat cgatagccgt gccgtcgacc tccagtcgtg cacggggcgc ggtcccgaga
     2461 tgtagcggtc cggccgcgtt gaatgagctg cttgtcgtgg ttactcgtgc cgacagcttg
     2521 agctcaggaa gctttccgat gagttcgagt tgagcgctgg cttgttcgag cagtgggtac
     2581 tcgggaaaga gggctcgcgc gtcgttcggt tgcacccgcg ggagatcgag cgcgatcccg
     2641 acgtttccgc cctgagcccg cacctctgcc ccaaactgaa tggcttcgtg aaagccatcg
     2701 aaagagaccg cgagcggtcc gggtgtgcgc acgtgcacgc tcgcgacgcc gcgcgtgtcc
     2761 gcgccgccga agccgcgcaa caccgcgccg aagcggtcaa agcgcagctc cgcccccgac
     2821 tgcgtggcga gtacccagcc gcgcgcttga ctgatctcga gctccaaggt gggcagaccc
     2881 gcgggcgcgc ctcgcgcgta accacgattc agcgctatgc tgcgcactcc cacgcgcacc
     2941 gggcgcgcgg cgctgctctg gggtgccgca gggctgggcc gtggggtgaa agtttggagc
     3001 agactcggcg tgccgtcttg gctctcgtac cagtgggctt ccacgctcgc gagctgagcg
     3061 tgttcgatgg cgatggtcag tggcgcatcg gtgtcagtga acagtttgcg gaccagagtg
     3121 attaggtcca cgctgagcgc gacgcgtgac gcgatcagga ctcggcgtcc ttgcgggtcg
     3181 tgggcactaa agccgttgac gatcagattt tgaggaccaa tctgctcgat ctctccgact
     3241 tcgaagcgtc cttgcagatt ctgattcagc gcggaggtcg cgaggcgccc gagcacccgc
     3301 cggctttgcg gcaaattcag gtgcagaatc acccccacgc cggaagcgac gaaaaagacg
     3361 gcgagccaga tcaacaacgt cgcggcgcgc cagaaagttc gccggtcggt cacggcatct
     3421 tcgccgcgtg cgcgaggtgg ggcgccgctc ctgccacgct gtcttcggtt cgcgtccttg
     3481 tcgatagtat ctttcggtta gcagaaaaga cgcgaccgag gggaggccga tgcccagttt
     3541 cggccgcacg ctcgagtacc agggctggca agcagggctg gcaagctcgc cccgacccag
     3601 cgtcaatccg ctgagtcgac ggcggccagc aatccctcct gagtcatgtc cgtgccgcca
     3661 atccagcgca cacgtcgggc tgaatcgact accaccacac tgggcagggc ctcgacgttg
     3721 aacttgttgg cgagcactcc atcgcggtcc actacgattg ggaatctcaa ctgcattcgt
     3781 gcggcaatgg cgcgagccgc ggcgtccgat tc
//
LOCUS       DASTKU010000689         3784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1694051, whole genome shotgun sequence.
ACCESSION   DASTKU010000689 DASTKU010000000
VERSION     DASTKU010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1694051"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFQ61_23980"
     CDS             <1..318
                     /locus_tag="VFQ61_23980"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957586.1"
                     /translation="ARHAEFATMERRVERRGGRMYLDTGQTGASRTIVAPYSVRARPS
                     ATVSTPLFPHEVSAALSPERFTMLTVPSRIAEFGDPFEALFEASVDVAAALGKLERWV
                     PRG"
     gene            476..1756
                     /locus_tag="VFQ61_23985"
     CDS             476..1756
                     /locus_tag="VFQ61_23985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9957587.1"
                     /translation="MRRTPAFGLRAALACVGVCAIGSGCSASATNCAPCQAASNPAAV
                     TASSGAGRPLSVCSFEHPGTPSEPDAQGLVRIPVSHPNLAYLGRVDCARDGAVGFAHA
                     GGIVRFAFKGSALDFLYRDHGGNGETDTNYFAVVVDQRAPQVIAMNSKQEVYPIARDL
                     PPGEHQVEIFKRTESGQLGKPGIGRGEFLGLRVPQGSEILPARPHAHRIEFIGDSITC
                     GFGTEATTSEPDKVHYSSRNSSAFSAWAAITARTLDADLMLVCYSGRGAYRNYSGLAG
                     DPLPVLYPRIDPDDSKSKPWDFSKFTPEVVVINLGTNDFSPGGVDRAKFRDAYAKFLA
                     TLRTTYPDATFILAVGPMMSDFYPPNEKAWSSIQEDLNTLLQQRRAAGDSNVHPLILP
                     TQTGPFGEDWHPTVMQQASMANQAVQLIRAVRGF"
     gene            complement(1799..2926)
                     /locus_tag="VFQ61_23990"
     CDS             complement(1799..2926)
                     /locus_tag="VFQ61_23990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957588.1"
                     /translation="MKHAEHHGYEVVVGQLSPGAALDQYVVKGCLGNGGMALVYEAED
                     TVLCRPVAIKMLSVLWSADSSVRSRFMREARAAATLRHPNVVAVFEVGRYRETPYLVM
                     ECLQGESLSQLLLREGPRSVQQTADLLLPVLSAMAAAHALGIVHRDLKPENIFLSRSE
                     SGVITPKVLDFGISKMLLPSHDSDITRSDVLLGTPNYMAPEHVEGKEQIDARSDIFSL
                     GVVMYECVTGERPFAASSLHAILEAIVSHHPPPPSELSAKVSREFSDIVMRALSKKAD
                     RRYPDVRELGAALLPFASARTKLMFEQDFVPSAAPQRAGRRLARSLRRASYLGLGLAL
                     LGGAAWVSYHFGKGRVEPTGRAEVERDRTAVPTTHESVEQR"
     gene            complement(3213..>3784)
                     /locus_tag="VFQ61_23995"
     CDS             complement(3213..>3784)
                     /locus_tag="VFQ61_23995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957589.1"
                     /translation="GDQAIVASICPKVVRSANPDSDPNYGYNPAIAALLKQLVSRLSG
                     RCLPRPPSLQPSGETACAVIEARLDGKCDCQSANRTETSPDALLRIRENLERDGLCKG
                     NACDSVCACGIPEARDQALEACRSTAPPEEQSPSFCYVEPDAGLGDPALVRDCPANQR
                     RLLRFVGQNTPAKGSLTYMACFGATSNAN"
BASE COUNT          640 a         1212 c         1217 g          715 t
ORIGIN      
        1 gcgcgacacg cggagtttgc gaccatggag cggcgcgtgg agcggcgcgg aggccgcatg
       61 tatctggaca ccgggcagac gggcgcttca cgcaccatcg tggcgcccta ctcggtgcgg
      121 gcgcgccctt cggccacggt ctcgacaccg ctcttccctc acgaggtctc ggccgcgcta
      181 tcccccgagc gctttacgat gctcacggta ccctcgcgca tcgcggagtt cggcgatcca
      241 ttcgaagcgc tgttcgaagc ttcggtcgac gtcgcggcgg cgctgggcaa gctggagcgc
      301 tgggtgccga ggggctgacc cgcgttcgtg cgatcctaat cgctagccgc gacgcgggtt
      361 gtgccctaat ctcggtcacc cgttttctag caggtgagag atggcttccg acgagatggc
      421 ttgtggcaaa gcagctgggc aactgaacct tcggaagatg cgaccgagga agagagtgcg
      481 tcgcaccccg gctttcggct tgcgtgcggc cctcgcttgc gtcggggtct gcgcgatcgg
      541 atccgggtgt tcggcgagtg ccacgaactg cgcaccctgt caggccgcga gcaaccctgc
      601 cgcggtcacg gcgagcagtg gtgcggggcg cccgctctcg gtgtgcagct tcgagcatcc
      661 cggcacgcct tcggagccgg acgcgcaggg gctggtgaga attccggtgt cacatcccaa
      721 cttggcctat ctgggccgcg tcgattgcgc gcgcgacggc gcggtaggct tcgcccacgc
      781 cggaggcatc gtgcgttttg cgttcaaggg cagcgcgctc gattttcttt atcgcgatca
      841 cggtggcaac ggggagaccg atacgaacta cttcgcagtc gtagtagacc agcgcgcccc
      901 ccaagtaatc gcgatgaact ccaagcagga ggtgtacccg atcgcccgtg atctgccgcc
      961 cggtgagcac caggtggaga tcttcaaacg caccgagagc ggccagctgg gtaagccggg
     1021 catcggccgc ggagagttct tgggtttgcg cgtgccgcaa ggttccgaga tcttgccggc
     1081 gcggccccac gcgcatcgca tcgagttcat cggggattcc attacgtgcg gcttcggtac
     1141 cgaagcgacg actagcgagc cggacaaggt ccattactcg agccgtaact ccagcgcgtt
     1201 ctccgcgtgg gcggccatta ccgcgcgcac gctcgacgcc gatttgatgt tggtttgcta
     1261 ctctggccgc ggcgcatatc gtaattactc ggggctggcc ggggatccgt tgccagtttt
     1321 gtatccgcgc atcgatccgg acgattccaa atccaagcct tgggattttt cgaagttcac
     1381 tccggaggtc gtggtcatca atctcggtac caacgatttc tcacccggag gggtagatcg
     1441 tgccaagttc cgtgacgctt acgcgaagtt tctcgccacc ctgcgcacca cctatcccga
     1501 cgcgacattc atcctcgcgg tcgggcccat gatgtccgac ttctatccgc ccaacgagaa
     1561 ggcttggagc agcatccagg aagatctcaa cacgttgttg cagcagcggc gcgcggccgg
     1621 ggacagcaac gtgcacccgc tgatcttgcc cacccagacg gggccgttcg gcgaagattg
     1681 gcacccgacc gtcatgcaac aggcgagcat ggccaatcag gcggttcagt tgatccgggc
     1741 cgtacgcggc ttctaagcga ctccaatcgc gacggcggtt tcgcggtcga gccgccgctc
     1801 aacgttgctc gaccgattca tgggtggtgg gaacagccgt tcgatctcgc tcgacctcgg
     1861 cgcggccggt gggctccacg cgccctttgc caaagtgata cgagacccac gctgcgcctc
     1921 ccaatagcgc caagccgagg ccgaggtaag acgcccgtct caagcttcgc gctagccgtc
     1981 gtcccgcgcg ctgaggcgcc gcgctcggta cgaaatcttg ctcgaacatc agcttggtgc
     2041 gcgcgctcgc gaacggcaat agggcggcgc cgagctcacg tacgtcggga taacgtcgat
     2101 ccgctttctt gctgagcgct cgcatcacga tgtccgaaaa ctcgcggctc accttcgcgg
     2161 agagctcgct cgggggcggc ggatggtgcg atacgatcgc ctccaagatg gcatgcaacg
     2221 agctggcggc gaacggccgc tcccccgtca cgcactcgta catcacgacg ccgagcgaga
     2281 atatgtccga ccgcgcgtcg atctgctctt tgccttcgac gtgctcgggg gccatgtagt
     2341 tcggcgtccc gagcagcacg tccgatcgcg tgatatccga gtcgtggctc ggcagtagca
     2401 tcttagagat cccgaagtcg agcaccttgg gcgtgatcac accgctttcg gagcgactca
     2461 ggaagatgtt ctccggcttc aaatcgcgat gcacgatgcc gagggcgtgg gcggccgcca
     2521 tcgcggacaa cacggggagc agcagatccg cggtttgctg aacggagcgt ggcccttctc
     2581 gcaacagcag ctgcgacaag ctctcgccct gcaggcactc catcaccagg tagggtgtct
     2641 cccggtagcg cccgacttcg aatacggcta ctacattggg gtgccgcagg gttgcggcgg
     2701 cgcgtgcctc cctcatgaag cgcgatcgca cgctgctgtc ggcggaccac agcacgctca
     2761 gcatcttgat cgcgaccggg cggcagagga cggtgtcctc cgcctcgtac accagcgcca
     2821 tcccaccgtt cccgagacaa ccctttacga catactggtc gagcgcggcc ccgggggaaa
     2881 gctgcccgac gacgacctcg taaccatggt gctcggcatg cttcatggca tgaactcgcg
     2941 ccgagtgctc ggggaacgag agctgcggag acgcggcgtg cgacaccagt agcggtttcg
     3001 caccctttgc tctgcgatca cggcttcgag atccacggct tcgagatcca cggcttcgag
     3061 agctattgtg accttcgata tcgtgctggc cacaatctgt ttgtgcccct ccagccgcgg
     3121 actcgtgcgt cgttttgccc cggggttccg gggtgggagc tggttgtctc catgactaca
     3181 gcagagagat tcttgccgac tcgggaggcc gctcaattcg cgttactcgt tgctccgaag
     3241 caggccatat acgtcagaga gcctttggcc ggtgtgttct gcccgacgaa gcggaggagg
     3301 cggcgttgat tggcagggca atcacggacg agcgccggat ctcccagccc ggcgtcgggc
     3361 tccacgtagc agaagctcgg gctctgctcc tcgggagggg cggtactccg gcaagcttcg
     3421 agcgcttggt ctcgagcttc gggaatgccg catgcgcaaa ccgagtcgca ggcgttccct
     3481 ttgcagaggc cgtcgcgctc caggttctct cggatccgga gcagcgcgtc ggggctggtt
     3541 tcggtgcggt tcgcgctctg gcaatcacac tttccgtcca agcgggcctc gatcaccgcg
     3601 caggccgttt caccactcgg ttgcagactc ggcggccttg gcaagcagcg accactcagg
     3661 cgcgagacca gttgcttgag cagcgcggcg atcgcgggat tgtagccata attggggtcc
     3721 gagtctgggt tggccgaacg caccaccttg gggcaaatcg aagctacgat ggcctggtcc
     3781 ccta
//
LOCUS       DASTKU010000690         7258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_800223, whole genome shotgun sequence.
ACCESSION   DASTKU010000690 DASTKU010000000
VERSION     DASTKU010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800223"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VFQ61_24000"
     CDS             <1..981
                     /locus_tag="VFQ61_24000"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957590.1"
                     /translation="ESAARSFENHRIAALERGLELHGHFRDAATGELSGVTLGGKYRI
                     LRKIGSGGIGAVYAAEHIALGSHVAVKVLRGAAAADASEIARLRREAKVQVSIEHPNV
                     VRVLDLDQLPDGSIYVVMELLQGRSLAARLKALGPLTPGEAVPIFRQVCRGLSAAHQL
                     GIVHRDLKPGNVFLCEDRNVKVLDFGMSKFAEAEQLTQEGYTLGTPEYMSPEQCIGAQ
                     VDPRTDLYAFGVMMYEALTGVLPIAGKNRREFLELHQRAIPRSMRERCPELGIPQELD
                     EAVLKCLKKRAAERPAGARDLERMLAEVPLSGPLKLMDSQSPTGKSHRAG"
     gene            complement(1026..1811)
                     /locus_tag="VFQ61_24005"
     CDS             complement(1026..1811)
                     /locus_tag="VFQ61_24005"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET9957591.1"
                     /translation="MLDWLYAALLGLIEGVTEFIPVSSTGHLLLAEQFLPRQTDLFNV
                     VIQCGAVIAVIPLFWARIRSLLLAYREPAGRDYLLKLMLAFGITAVGGLLLQKGGYEL
                     PESALPVALALLIGGIAFLAIEHLVRGREPSDRITWTVAVLMGVGQLIAAVFPGASRS
                     GSTILLALLVATSRPAATEFSFILGVPTILAAGGVKILKAFSASAPVEHWDRVAIGTL
                     VSALVSFVVVRWFLRYVRSHSFSLFGWYRIALGLIILAAMTAH"
     gene            complement(1896..3287)
                     /locus_tag="VFQ61_24010"
     CDS             complement(1896..3287)
                     /locus_tag="VFQ61_24010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET9957592.1"
                     /translation="MSDVDARASLPAGSAPHLWGVTWPLLCSLALSLSLHFTDSFFLA
                     HISDQAAAAAGALFPLLGAAIVVFSAVGQAGGSVASQLSGARRFDELPVTYLTLVGFN
                     TLLGLSSSLGFVLFHNALPRWLGLSGTVADQAAAFLVVVGGGQFLKAVQIAYGNILNS
                     RRQTKWAFVEALITNFCNITLNVALLRGWFGLKQSVTGIAWVTVVSLGIGMLFTISIV
                     HLKLRVRFALGRNRAHFSERLRRVLGIGLPSALEPISYQFAQMTVNAIIIPWGASALA
                     TRTYVFNFYMVTTVLLAVAFGIGTQVMVSHAVGAEDFRSANAEMRRGLAFGVIGNFVL
                     AGMLVLFHRPLLGLLTRDPSIHRFAGPLFLLGMAVEPGRAVNIVAGGALRSSGDARYT
                     ALIGICLMWTVGMPLCFFFGRYLGLGLTGIWVAFACDEILRGVVNYRRWLTGHWKEYR
                     ISLRPRPESLEPS"
     gene            3494..4138
                     /locus_tag="VFQ61_24015"
     CDS             3494..4138
                     /locus_tag="VFQ61_24015"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9957593.1"
                     /translation="MPPSHASYPAVLRPRSPGPFSEPGRAAALAKAAAEGDVTATREF
                     LTNVSPALHRVVTSVLGAKHPDREDVVQQAMIALVHALAAFRGECHPVGYASRIALHV
                     ALRARRRARVERGHQETLERLSREFEEPHADPDDGMAERRRRALRDLLEELPEEQADA
                     LALRVMLGWSLEETADAAGAPVNTIRSRVRLAKQALRKRIEANPELFDDLEVLP"
     gene            4135..5376
                     /locus_tag="VFQ61_24020"
     CDS             4135..5376
                     /locus_tag="VFQ61_24020"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9957594.1"
                     /translation="MSGLDLHPEDLLDKFADRTLSEAEAARLDAHLAMCSVCRVELQL
                     RADFEEESELLRPNAPLQLTPLPAVARGIAAPLSATAPLQGSASPHAGPASAASNQER
                     AVRDERSAMGESKQTVRRRRPLAARRLAWVAAALLSLVASVSLAARYTGWFESRGAAN
                     RPILNSNLQGTGARGHGPRSASKAEQPTVMPTPSSAIEASPGREGSVTVPSASSALDQ
                     AAGGESPSGVRVVRPSVVSNRSVPNRPVSDPAPLRTPQRRGERSATVETSNRAPAANG
                     GSPHSAALENVEADASALFREANAARRGGNAVLAIELYRRLQRVYPASPEAELSRATL
                     AALLLEQGEPAAALDSFDKYLQRGGKLVDAEALVGRALALSKLGKRDEEIAAWRAVLS
                     KFPGTVYAEQATKRLTALGER"
     gene            5386..6564
                     /locus_tag="VFQ61_24025"
     CDS             5386..6564
                     /locus_tag="VFQ61_24025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957595.1"
                     /translation="MLAATDPHRFRVAVRVIGVLLLSLSASAAARAEGTATSGSPSAE
                     SSLLRVELWVYGSADAVARARLIGEELVQRLPVRIDVHEARAPEAGARMSESRADVKF
                     EVDLRSLSRVSVVMFDGAGSRELSRRVLPEASSLEVSTEATSYVISTQLQTYLRTRAP
                     RESRPPVAKPPARAPEARKRQTPDTVSSNAPAFFQAEIEAQGRLGWLHADQASVGFAL
                     GALARARRPGLSPQLQLLLAGYLPERISRGELVANWWLGSLRLLPGFEWRFGPSLSFV
                     LGLGGGVDWFRLTPVDDPDRAVRGNAVRRVTPVLTGVLGLGLSFVGAGRWLIGVNSDL
                     ELAPTRYVAEAGPERRTFLIHNGLRPQVFSGVSFPLGAAPESPRARSAGPSARGATND
                     "
     gene            6557..>7258
                     /locus_tag="VFQ61_24030"
     CDS             6557..>7258
                     /locus_tag="VFQ61_24030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957596.1"
                     /translation="MTKVRSALSWLTLGSSVLAVGASCRENELVLFEIGGAPVAIASS
                     SAGSGGGGGSSAPPTSQSTTPAPGNCESSSDCPNGWLCVKSDCAVTSGACEPPPLFCD
                     AGREPVCGCDGVTYWNDCVRRQNLSAASTSGECRGSAQRCESASDCPAQNAFCAQLMP
                     PGRGCDLEQAGTCWVLPTDCDPSLDSARWMSCDFGRPRPGGPQGSGFPMDGPPQNSGF
                     PDGGGSGGPDATCRDT"
BASE COUNT         1305 a         2241 c         2501 g         1211 t
ORIGIN      
        1 gagagcgcgg cgcgctcctt cgaaaaccac cgcatcgcgg cgctcgagcg cgggctggag
       61 ctgcacggtc attttcgtga tgccgctacg ggtgagcttt ccggggtgac gctcggcggc
      121 aagtaccgca tcctgcgtaa aattggctcc ggaggcatcg gcgcggtgta cgcggcggag
      181 cacattgcgc tggggtctca cgtggcggtc aaggtgctgc gcggggcggc ggcagcggat
      241 gcttccgaga tcgcgcgtct gcgccgggag gccaaggtgc aggtctcgat cgagcatccc
      301 aacgtggttc gagtgctcga tttggatcag ctacccgacg gctcgattta cgtggtgatg
      361 gagctgcttc aggggcgcag tttggcggcg cgcttgaagg cgcttgggcc gctcacgccg
      421 ggcgaggcgg tgccaatttt taggcaggtg tgccgtgggc tgtccgcagc gcatcagctc
      481 ggaatcgtgc atcgcgacct gaagcccggg aacgtattcc tgtgcgagga tcgcaacgtg
      541 aaggtgctcg acttcggtat gagcaagttc gcggaggcgg agcagctgac ccaagagggt
      601 tacacgctcg gtacgcccga atacatgtcg cccgagcagt gcatcggggc gcaggtggat
      661 ccacgcacgg atctgtatgc gttcggggtg atgatgtacg aagccctgac gggagtactg
      721 ccgatcgcgg gcaagaatcg gcgagagttc ctggagttgc atcagcgcgc cattccgcgc
      781 tcgatgcgcg agcgatgccc ggaactcggg atccctcaag agctcgacga agcggtgctg
      841 aagtgcctga agaaacgcgc ggcagagcgc ccggccggcg cgagagatct agagcgcatg
      901 ctggccgagg ttccgctcag cggccccctg aagttgatgg acagtcagag ccccacgggc
      961 aagtcgcacc gagcgggcta atcaaccgcc tctgctgcga gcgacgccgg ggtcgcgcaa
     1021 ttacgtcagt gcgcggtcat ggccgcaaga atgattaggc cgagcgcgat gcggtaccag
     1081 ccaaagaggc tgaagctatg gctgcggaca tagcgcagga accagcgcac cacgacgaac
     1141 gacacgagcg ccgacacgag ggtgccgatg gcgacccgat cccaatgctc gacgggcgcg
     1201 gaagcggaga atgctttcag gatcttgacc ccgcctgccg ccaaaatggt cggcacgccg
     1261 aggatgaacg aaaactcggt cgcggcgggg cggctggttg ctaccagcag ggccaacaag
     1321 atggtggagc cagaacggga ggctcccggg aacaccgccg caatgagctg acccacgccc
     1381 atcaatacgg cgaccgtcca ggtgatccga tcggatggtt ctcgcccgcg caccaggtgt
     1441 tcgatggcga ggaacgcgat acctccgatc agcaaggcca aggcgaccgg gagcgcgctc
     1501 tccggcagtt catagccgcc cttctggagc agcaggccgc cgaccgccgt gatgccgaag
     1561 gcgagcatca gcttgagcag gtagtcacga ccggcgggct cgcggtacgc gagcaagagg
     1621 gagcggatgc gcgcccaaaa caacggaatg accgcgatta ccgcgccgca ctggatcacg
     1681 acgttgaaca gatcggtctg gcgcgggaga aactgctcgg ccaacagcaa gtgtcccgtg
     1741 gacgagaccg gaatgaactc ggtcacacct tcgattagac cgagcaacgc cgcatacagc
     1801 cagtccagca tgtcggggcc agacttatcg cgatgtctgc tggaaggcta gggtgagcgg
     1861 ccgtgagaag ggccgcggcg tgttgcccaa gtgattcagg agggttcgag cgactcgggg
     1921 cgaggccgaa gcgagatgcg atactccttc cagtgaccgg tgagccagcg tcggtaattc
     1981 acgacgccgc gcaagatctc gtcgcaagcg aaggcgaccc aaattccggt cagcccgaga
     2041 cccaggtaac gaccgaagaa gaaacagagc ggcatgccga ccgtccacat caggcaaatc
     2101 ccgatcaagg ccgtataccg tgcatcaccc gatgagcgca gcgcgccgcc tgccacgatg
     2161 ttcacggcgc gccccggttc cacggccatg ccgagcaaga acagcggccc tgcgaaacga
     2221 tggatggaag ggtcacgtgt cagcagcccc aaaagcggtc ggtggaacag caccaacatc
     2281 cccgccaaca cgaagttccc aatcaccccg aacgcgaggc ctcgccgcat ttccgcgttg
     2341 gcgctccgaa agtcttctgc gccgacggcg tgggagacca tgacctgcgt gccgataccg
     2401 aaggccacgg ccaggagcac ggtcgtgacc atgtagaagt tgaagacgta ggtgcgcgtg
     2461 gcgagcgcac tggcgcccca agggatgatg atcgcgttca cggtcatctg cgcgaactgg
     2521 tacgaaatgg gctcgagcgc cgagggtagg cctatgccca gaacgcgtcg caggcgttcg
     2581 ctgaaatgag ctcggtttct gcccaaagcg aagcgcaccc ggagcttcag atggacgatg
     2641 ctaatcgtaa agagcatgcc gatgccgagt gagaccaccg tcacccaggc gatgccggta
     2701 accgattgct tcagcccgaa ccagccccgc agcagcgcta cgttgagcgt gatgttgcag
     2761 aagttcgtga tcagggcttc gacgaaggcc cacttggttt ggcgtcgcga gttcaggatg
     2821 ttgccgtagg cgatctggac ggccttcagg aattgtcctc cgcccaccac gaccaagaat
     2881 gcagccgctt gatcggcgac cgtccctgac aggcccaacc aacgtggcaa agcgttatgg
     2941 aagagcacga agcccaggct cgaactcagg cctagcaggg tattgaagcc gaccagcgtc
     3001 aggtaggtga cggggagctc gtcgaagcgg cgcgcgccag acagctgcga ggccacactc
     3061 ccgccggctt ggcccaccgc ggaaaatacc actatcgctg cgccgagcag cggaaacagc
     3121 gctccggcgg ctgcagcggc ttggtccgag atgtgagcga gaaagaagga atcggtgaag
     3181 tgaagcgaga ggctcagcgc caggctgcag aggagcggcc aagtcactcc ccagaggtgc
     3241 ggagcagacc ccgccggaag ggaagcacgg gcgtctacgt cagacatgtt ggcctcgcgg
     3301 cgattaccca gttcggcggg ggcgcgcaac caaaccccca aatttgagca gattcgcgga
     3361 tgtttggggg ctgaggcccc ggattgaacc cccggttagt agacggtgcg gccacggcgg
     3421 cgaacgtgga tcgacttcgg tatcgagact ccattgatcg ctcgtcgggc atcccggaca
     3481 ctttaagtac gcaatgcccc cctcccacgc gagctatcca gcggtgttgc gtccccgttc
     3541 cccggggccg ttctccgagc cggggcgcgc ggccgcgtta gccaaagcgg ccgcagaggg
     3601 agatgtcact gccacccgcg aattcctgac caacgtctcg ccggccttgc atcgcgtggt
     3661 cacgtccgtg cttggtgcca agcaccccga ccgagaggac gtggtgcagc aagcgatgat
     3721 cgcgctggtg catgcgctgg cagcgttccg cggggagtgt cacccggtgg ggtatgcttc
     3781 gcgcatcgct ttgcacgtgg cgctgcgcgc ccgacgtcgg gcgcgcgtcg agcgtgggca
     3841 tcaggagacg ttggagcgcc tgtctcgcga gttcgaagaa ccgcacgccg accccgacga
     3901 cggcatggcg gaacgtcgtc gccgcgcgct gcgcgacctg ctcgaggagc tccccgagga
     3961 gcaagcggac gcgctggctt tgcgggtaat gctcggctgg tcactggaag aaacggcgga
     4021 cgcggcgggc gcccccgtga atactattcg tagccgggtg cgactcgcca agcaggcgct
     4081 gcgcaaacgc atcgaggcga accccgagct gttcgatgac ctggaggttt tgccgtgagc
     4141 gggctcgatc tgcatccaga ggacttgctg gacaagttcg ccgatcggac gctgagcgaa
     4201 gcggaagccg cgcggctcga tgcccatctt gcgatgtgca gcgtatgccg cgtcgagctg
     4261 cagctgcgtg ctgacttcga agaagagagc gagcttctgc gaccgaatgc cccgctgcag
     4321 ttgaccccgc tgccggccgt ggcgaggggg atcgcagcgc cgttgagcgc caccgcgccc
     4381 cttcagggga gcgcatcacc gcatgccggg ccggcgtctg cggcgtcgaa ccaagaacgc
     4441 gcggtgcgcg acgagcgctc cgcgatgggt gaatcgaaac agacggttcg gcgcagacga
     4501 cccttggcgg cgcgccggct tgcttgggtg gctgcggcgt tgctctcgtt ggttgcatcc
     4561 gtcagcttgg cggctcgtta taccggctgg ttcgagtcgc ggggtgcggc gaaccggccg
     4621 atcttgaact cgaacttaca gggcaccggc gcgcgcggtc acggccctcg ttcagcgagt
     4681 aaggctgagc agcccacggt aatgccgact ccctcgtccg ccatagaggc gtctccgggc
     4741 cgcgagggtt cggtgaccgt ccccagcgct tcgagcgctc tcgaccaagc cgcgggcgga
     4801 gaaagcccga gcggggttcg agtggtgcgg ccttcagtcg tttctaaccg gtctgtcccc
     4861 aaccggcctg tgtccgaccc cgcccctctg cggacgccac agcggcgcgg cgagcgttca
     4921 gcgacagtcg aaacatcgaa tcgggcaccc gctgcgaacg gtggctctcc acactcggcg
     4981 gcgctcgaga atgtcgaggc ggatgcttcc gctctattca gggaggccaa cgcggcgcgg
     5041 cggggcggca acgccgtcct cgcaatcgag ttgtatcgac gcttgcagcg cgtctatcca
     5101 gcttctcccg aggccgagct gtcgcgtgcc acgctagcgg cgttgctcct cgagcagggt
     5161 gaaccggcgg cggcgctcga tagcttcgac aagtatttgc aacgcggagg caaattggtc
     5221 gacgcggagg cgctggtcgg acgagcgctc gcgctttcca aactcggcaa gcgcgacgag
     5281 gaaattgcgg cctggcgcgc cgtgctcagc aagttcccgg gcaccgtgta tgccgagcaa
     5341 gcgaccaaac gcctgacggc gttgggtgag cgctgatcta caccgatgct cgccgcgacg
     5401 gatccccatc gatttcgtgt tgccgtccgc gtgatcggcg tgctgctgct ctcgctgagc
     5461 gccagcgcgg cggcgcgagc ggaagggacc gcgacttctg gctctcccag tgcggagtcc
     5521 tccctcctgc gggtggagct gtgggtttat gggagcgccg acgcggtagc gcgagcgcgg
     5581 ctgatcggcg aggaactcgt gcaacgcttg cccgttcgaa tcgacgtgca tgaagcgcgc
     5641 gcgccggagg ctggcgcgcg aatgagcgag tcgcgggctg acgtgaaatt cgaagtcgat
     5701 ctacgttcgc tctcgcgagt gagcgtcgtg atgttcgacg gcgcgggtag ccgcgagttg
     5761 tcacgtcggg ttttacccga ggcgagctca ctcgaggtct ccacggaggc taccagctac
     5821 gtaatctcga cgcagctaca gacttacttg cggactcgag ctccgcgcga gagccgcccg
     5881 cccgtcgcca agccccccgc ccgagccccg gaagctcgaa agcggcagac tccagacact
     5941 gtctcttcga atgctccggc tttctttcag gccgagatcg aggctcaggg ccgcttgggg
     6001 tggctccacg ctgaccaggc gagcgtggga tttgccctgg gcgcgctcgc gcgcgctcgg
     6061 cgcccgggct tgagtccaca gcttcagctg ttgctagcgg gatacttgcc cgagcgcatt
     6121 tcaaggggcg agttggtggc gaattggtgg ctcgggtctt tgcgcctact gcctggcttc
     6181 gagtggcgat tcggtccctc gctgagcttc gttctcggtt tggggggcgg ggtcgattgg
     6241 ttccggctca cacccgtcga cgacccggat cgtgcggtgc gcgggaatgc cgttcggaga
     6301 gtaacgccgg tgctcacggg tgttttgggc ctggggcttt cgttcgtggg tgccgggcgc
     6361 tggctgatcg gagtcaactc agacttggag ttggccccga cccgctatgt cgccgaagcg
     6421 ggccccgaac gacgcacctt cttgattcac aatgggcttc gtccgcaggt cttcagtggg
     6481 gtttcgttcc cgctcggcgc cgcgcccgag tctccgcgtg cgcgctcggc cgggccttca
     6541 gcgcgaggcg cgaccaatga ctaaggttcg aagcgcgttg agctggctca cgctgggctc
     6601 gagcgttctc gcagttggcg cgagttgccg ggaaaacgag ctggttctgt tcgaaatcgg
     6661 gggcgcgccc gtggcgattg cctcgagctc cgcgggctcg ggcggaggcg gcggcagcag
     6721 cgcgccccca accagtcaat ccacgacgcc tgcaccaggg aattgtgaga gctcgagtga
     6781 ctgtcccaat ggctggcttt gcgtaaagag cgactgcgcc gtcacgagcg gggcctgtga
     6841 accgccgccg ctgttctgcg atgcagggcg agagccggtg tgtggctgcg acggagtgac
     6901 ttattggaac gattgcgtgc gccgccagaa cctgagcgcc gccagtacct ccggcgagtg
     6961 tcgtggatcg gctcagcgct gcgagagcgc cagtgattgt ccggctcaga atgcgttttg
     7021 cgcgcaactg atgcctccgg gacgtggctg cgatctcgag caggccggca cttgttgggt
     7081 gctgcctacg gactgcgatc ccagcttgga ttcggcgcgt tggatgagtt gtgatttcgg
     7141 tcggccccgc cccggcggac ctcaaggtag cggctttccc atggacgggc cgccgcagaa
     7201 ctcgggcttt cccgacggcg gcggatcggg cggacccgac gcgacgtgca gagatacc
//
LOCUS       DASTKU010000691        11666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_800265, whole genome shotgun sequence.
ACCESSION   DASTKU010000691 DASTKU010000000
VERSION     DASTKU010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11666
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800265"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            657..1097
                     /locus_tag="VFQ61_24035"
     CDS             657..1097
                     /locus_tag="VFQ61_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957597.1"
                     /translation="MARSLIWSLAAAAVLVSGLGCGNDDGDEHPEEPPSVPIPGTPGS
                     GIGVAQGDGTGVGGAGTAGNASSGGSTAGTTAGDGTNPSGFGGNAFGGSGGNTTAGNP
                     GAGGSGGLGSGGTQNGGGNSGTGNTPGVAGSMGLGTGGTNSFGT"
     gene            1346..3730
                     /locus_tag="VFQ61_24040"
     CDS             1346..3730
                     /locus_tag="VFQ61_24040"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9957598.1"
                     /translation="MRSTDGFSQLSKRGRGPRSDSGDPALVIPNKLALRTSLIYGAVG
                     ALWILCSDALVAAIFREREAITRVSMYKGWAFVVVTALALYTGLRGQLEQLKAEHRRR
                     ELAEAERRRGEHEIARLSRVYATLSRINECVARARDRTALFEQVCSIATGEAEYKLAF
                     VAWAATPSSRLSMQALSGECEHFLDMNEVLNSNPIVDAFENGRRAIAELNLQSSAACE
                     GCMKCAGLRSAAAFPIRVRGEVHGVFAACASEAGAFGASELELLERAIDNVCFGLEML
                     DREERRARAERALQSNEDRLRRMADSVPAGLVILSGLRFDFVNDHARKLLGAADQQAL
                     DGHSIAEYLHTDDQSAFSQAIDELLARGTPSSFSELRLLRADGVVVLAEVSAVPFDAG
                     GDPTTLVFFRDIGKQRLLEQQLRQSQKMEAIGTLAGGVAHDFNNLLTVILANASLLLP
                     ALEQDASNRDLVQEITETGTRAAELTRQLLLFSRRQALRSTEFDVNDVVANLSKLLRR
                     ILGEDVAFCVECCPGLPAVRGDVTMFEQVIMNLAVNARDAMQDGGKLTISTRTEQSAR
                     PATPMQPYPKPMPCVVISVSDTGTGIGADILPHIFEPFFTTKDVGQGTGLGLATAYGI
                     VDQHHGWIAVDTRVGTGTTFHVYFPAVSVRQPSPPVEDRARGLAGGKETLLVVEDDPA
                     VRRAIVGVLRHCGYTVLEAASGPDALALWAQRQTDIKLLITDCIMPLGINGLDLADRL
                     RHDEPSLPVLYTSGYRAMGIDSSRLELGVNFLEKPFEANALAVLVRKQLDVSVI"
     gene            3809..4213
                     /locus_tag="VFQ61_24045"
     CDS             3809..4213
                     /locus_tag="VFQ61_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET9957599.1"
                     /translation="MKKTYRGSCHCKRVTFEAELDLSQGTGKCNCSYCWKVRNWSVIV
                     KPDAFRLLSGEDQLSDYQFGGKVGHHLFCKNCGVRTFGRGNIPEIGGEYCSVILSTLD
                     DLDPGELAEAPVRYFNGRDNDWFHTPSETRHL"
     gene            4299..4637
                     /locus_tag="VFQ61_24050"
     CDS             4299..4637
                     /locus_tag="VFQ61_24050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957600.1"
                     /translation="MDAIWRLMPPAAWAAFCLALAGVYARFWPRPLTQAGQTEMLGSG
                     GLGRVRYLVLRWFHSLVWLLLALAVVLRGEDQHTWAVRVATAALVVYVVFIVFSTMAR
                     RRERSASGPE"
     gene            4785..5171
                     /locus_tag="VFQ61_24055"
     CDS             4785..5171
                     /locus_tag="VFQ61_24055"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HET9957601.1"
                     /translation="MTHTVNRDAMSIEPHAIRARAYELWNERGRPDGSADHDWLRAEQ
                     ELMNRLSAKQPAATALPETSGATVSTPVAQDPTQARAAVNKTADKPSASRAKKSSRSK
                     VRDVSAEPGAAAATRLLAKLATNARA"
     gene            complement(5236..5943)
                     /locus_tag="VFQ61_24060"
     CDS             complement(5236..5943)
                     /locus_tag="VFQ61_24060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957602.1"
                     /translation="MRTSLSALALITIATTLVAGCGSAATTAHPNAPVASNAGDHSES
                     APTAKDAAAETPTVTPKEAAPAVAPPPSENDGIRKASRPPHDLIMAPDVLFVFNFAQS
                     EVGESAKQRCEESAGDDRAALRSCLEAARAKVPVESIQFTKDAHGAWWWVTYNRYKGN
                     LLKWNKVQFAPGEETTDQITLKLSGKDKGIAPMPRVPKTLAIDLPNDYSIVVKHPEFG
                     AMTYDAKIGMLAPDPDR"
     gene            6303..6815
                     /locus_tag="VFQ61_24065"
     CDS             6303..6815
                     /locus_tag="VFQ61_24065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957603.1"
                     /translation="MVLSVREFSSWLVLFGLLGAACEQGRVTGGPAFLSTDFTAEQTQ
                     SVVTALDEWRRATAHAVDFTPVLRNERDGEHVSVRPGRLGENEAGREWRGQIMIDVEL
                     LDRRGSGELGVTTVTMHELGHAIGIRAHTSEGLMQSDVSLAHPCIDAYTLELVCNWMA
                     CGPEAAPTCP"
     gene            complement(6845..7435)
                     /locus_tag="VFQ61_24070"
     CDS             complement(6845..7435)
                     /locus_tag="VFQ61_24070"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET9957604.1"
                     /translation="MIPVSVAAEFFAQTNLNQILAATRSGGETELATQQIDRLLAGRR
                     RGERCFTIQTQDDLLRVFQTITSAMSWALLAIASISLLVGGVGVMNIMLVSVRERTRE
                     IGIRRAVGATRGDILFQFLVESVLIALLGGGIGLAVGSILVLVVTYSAPQIPVRLSGS
                     TALLAFCSAFVVGATSGVMPARQAAQLDPSHALRFE"
     gene            complement(7605..8213)
                     /locus_tag="VFQ61_24075"
     CDS             complement(7605..8213)
                     /locus_tag="VFQ61_24075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9957605.1"
                     /translation="MSVGPARDVSAERTVGEEERRHTDIELMRFAAAGQRAAQRIVML
                     RLMRRIQRLCRSLLRHAEDARDASQASLFAILRSAANFRGESSLERWADRIAIRTTLR
                     QFREKRHDSFDSTEDACLLVVQASADPGIAASQYLDLLPERQRTALILRCGFEYSVEE
                     IAEITRTSPNTVKDRLKRGRENLRRAVGRDELLPMRAGQRPK"
     gene            8397..10334
                     /locus_tag="VFQ61_24080"
     CDS             8397..10334
                     /locus_tag="VFQ61_24080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957606.1"
                     /translation="MNHVDSFGHQTSVDRKIDSTIQGRAGENIGKPIANDVTNRVMQG
                     SMALLSSRAAVWWIGTTAAVLSLTSIQPLRALDDWVIALAVSGRGGEVGLPRSAWDAF
                     TFTRGDPAENAALRDAGLMLPWWADTQLRVAFFRPLAALSHALDERLWPNHPALMHVH
                     SLVWLVLLVALMSRVYRRFADSSGLAGLALALYALDDARGATLAWLSNRNALMCAVFG
                     CGALLAHDTWRQKRLAKAGIGAIALFLATWASGELAIGALAYLVAYALFVDRGSWRAR
                     ATSLMPYLLLSLICVVTTRSAGFGVQRSGAYLEPWFDISAFLQQAFERWLWLAASQLG
                     PVPSDLGWMLGPGAKSWLWLGAISVIGLFAWLLRPFLDDAKVRFWLCGGILSLLPMAT
                     TLPSDRLLTFVGIGASPLLARVLGPSLTGPSRRVRTALGYSFAAVHLILAPLLFPLRA
                     AQMNWIASAEETAYASLGAPLPEAKTLILVNAPHVLVTNYIQARWSYLKASHPAHVHS
                     LAATGSDVRVTRLGAQTLLVTPRSGYFDTPLERAYRSDFTALARAQSVRLTAFEAQVS
                     STTPDGRPRSIAFSFTRPLNEYLFACWVGGRFVPCDLPAIGRSAVFEADDLKVVVRSL
                     TRNSEPDSKAREFEMKGSRIE"
     gene            10331..11227
                     /locus_tag="VFQ61_24085"
     CDS             10331..11227
                     /locus_tag="VFQ61_24085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9957607.1"
                     /translation="MKDSELECVVCASVLRATRAESTRVRSNVRAFSHESFEVWRCRV
                     CSSIHAREPVDLGRYYAEYPFHGLREDLRTRIVYAKQLARLRRAGLQRCHRVLDYGCG
                     GGQFVRYLASRGYDSHGFDAYCPQYQSRSTLERQYDCVITQDVIEHVDSPNALLGVLR
                     SLTRPAGILCVGTPNAEAIDLRRAHEFIHTLHAPYHRHILGREALVQRAELHGFQLRA
                     FYRTMYTNTALFGLNEAFYRYYAGLLDDTLDAILGPIQVGPMLRRLPLTLFWGLFGGA
                     FSRGTDCTAIFSRQDSGLTQSA"
     gene            complement(11253..>11666)
                     /locus_tag="VFQ61_24090"
     CDS             complement(11253..>11666)
                     /locus_tag="VFQ61_24090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957608.1"
                     /translation="AWRERAMAQHNLHLPGSDVASPIAPSGALSAERLEVLASGQKLL
                     IYAILLNIATYALSRAMGPIVGLLAFPSVVLSVIGVYRIARGLGWSSTLGVVLAALTL
                     VPFVALVVLIVLNGRASRRLRDGGYRVGLLGASRV"
BASE COUNT         2149 a         3645 c         3642 g         2230 t
ORIGIN      
        1 gctctcctct ccaggttaag gggaggccct tgctccatct atcaactttt tctcgagaac
       61 gcggtcccac gctccagcat cgcaagccgg gtccctcgga tgacgcttcc ttgcgagcag
      121 aatcgaattt tcgtgctcgg agccagacct ccatcgcccg gctcggcgca cggcgccggc
      181 gtcgcgcctc tgcgaggccg ttttgctacg agaccccgtt cacgcctcgt cctggattgg
      241 gcccgcgctg tccgccggag ggacttggag cgtccagcac ggttcaacga cgcaacacgt
      301 tgcgccccaa tacctctccg gcagtaacaa gggcgcgccg gcgtggtgtc ttggcgagca
      361 cggacgaggt cgcggctgcg ctgctggaat gaggagacgc tgatcgcgct cgttgtagtg
      421 cggcgaaatc ggtaatggcg tggctccaga cagcgacccg gcacgattgt ggaacaggtg
      481 ttggcgcaga attgctcctt cgatccacgg atatgtgcac tcatgcctcg agcactcgtc
      541 cgggcacacg ttctgcatta gtcccgagcg gcagtttgtt cctcatcggc agtgctgaat
      601 gcgcccgaat cgcgagatcc gggcgcgaac cacggagatc acactcgagg tctactatgg
      661 ctaggtccct gatatggagt ttggccgcgg cagctgtatt ggtaagcggc ttgggctgcg
      721 gcaatgacga cggggacgaa catccggagg agccgccttc ggtcccgatc ccgggtaccc
      781 ccggcagtgg cattggagtg gcccaaggag acggcactgg agtcggtggc gctggcacgg
      841 cgggcaacgc atcgagcgga ggctctaccg caggcacgac ggcgggcgac ggtaccaacc
      901 cgagtgggtt tggtggcaat gctttcggtg gtagtggagg caatacaacc gctgggaatc
      961 ccggagcggg tgggagtgga gggctcggtt cgggaggcac ccaaaacggc ggtggaaatt
     1021 ccggtacagg aaacacgcct ggtgtcgccg ggagcatggg tctaggtacg ggaggaacga
     1081 attcgtttgg aacttgaccg aaccggactg aaccgtcatg gcgggaactg ccacccgttg
     1141 gcggtgagcg aatggcgcgc agatgctgca gagaggctgc tcacccaacg caccctgttc
     1201 gtagccaagg ctacgtgcca aaagcttgtc gccgatgcgt agtcgctaac cggccgaaaa
     1261 cacgtttaaa cggttgggga aacccggagg tgcggcacac ctgatattag aggcctgtag
     1321 cttgcagcgc agcctcgagg atcggatgcg gtcgacagac ggtttttccc agctcagtaa
     1381 acgcggccgt ggcccgcgat ccgactcggg cgaccccgcg ctcgtgattc cgaacaagct
     1441 cgcgctgcgc acgagcttga tttacggagc cgtcggcgcg ctttggatct tgtgctcgga
     1501 tgcgctcgtc gctgccatat ttcgagagcg agaggcgatt acgcgggtct cgatgtacaa
     1561 ggggtgggcc ttcgtcgtgg tgaccgcgct ggccttgtac accggcctgc gggggcagct
     1621 cgagcagctg aaagcggagc atcgacggcg tgaattggcc gaggcggagc gtcggcgtgg
     1681 cgagcacgag atcgcgcggc tcagccgcgt ctacgccacg ctgagccgca tcaacgagtg
     1741 cgtggcgcgg gctcgcgacc gaaccgcact gttcgagcaa gtttgcagca tcgcaaccgg
     1801 ggaggccgag tacaagctcg ctttcgtggc gtgggcggcg acgccgagtt ctaggctgag
     1861 catgcaggcc ttatccggcg agtgcgagca cttcctggac atgaacgagg ttctaaattc
     1921 gaatccgatc gtggacgcgt tcgagaacgg acgccgcgca atcgccgagc tgaatctaca
     1981 gagcagcgcc gcctgtgagg gatgcatgaa atgtgctggt ctgcgttcgg cggcagcctt
     2041 tcccatccgt gttcgaggcg aagttcacgg agtgttcgcg gcctgcgcga gcgaagcagg
     2101 cgctttcggt gcgtcggagc tcgagctcct cgagcgtgcc atcgacaatg tttgtttcgg
     2161 gctcgagatg ttggaccgag aggagcgtcg cgcgagagcc gaacgggcgt tgcaatccaa
     2221 cgaggatcgc ttgcgacgca tggccgacag cgtccccgcg gggctggtga tcctgagcgg
     2281 gttgcgcttc gacttcgtga acgaccacgc acgcaagctg ctcggggccg cggatcagca
     2341 ggcgctcgat ggccactcga ttgcggagta cttgcatacc gacgaccaga gtgcgttttc
     2401 ccaagccatc gacgagttac tggcgcgcgg cacgccaagt agtttctcgg agctgcgcct
     2461 gctccgcgcg gacggagtgg tggtgttggc cgaagtctcg gccgtgccct tcgatgcggg
     2521 cggggatccc accacgctgg tgttctttcg agacatcggc aagcaacggc ttctggaaca
     2581 gcaactccgg cagtcgcaga agatggaggc gattggtacg ctggcgggcg gcgttgccca
     2641 tgacttcaac aacttactga cggtgattct agccaacgcc tcgctgctct tgccggccct
     2701 cgaacaggac gcatcgaatc gagacctggt gcaggagatt accgagacgg gcacccgcgc
     2761 ggcggagctc acccgccaac tcctgctgtt cagtcgccgt caggcgctgc gttccaccga
     2821 gttcgatgtc aatgacgtgg tcgcgaatct ttccaagctg ctcagacgca tcctgggtga
     2881 ggacgtcgcg ttttgcgtag agtgctgccc agggctgccg gcggtgcgcg gcgacgtcac
     2941 gatgttcgag caggtgatca tgaatctcgc cgtcaatgcg cgcgacgcga tgcaggacgg
     3001 tggcaaacta acgatctcga ctcgtaccga gcagagcgcg cgcccggcta ctcccatgca
     3061 gccttacccc aagccgatgc cctgcgtagt gatctccgtg agtgacacgg gaaccgggat
     3121 cggagcggac atcctgccgc acattttcga gccgtttttc acgaccaagg acgttggaca
     3181 aggcaccggg ctcgggctgg ccactgccta cggcatcgtc gatcagcatc acggctggat
     3241 cgccgtcgac acgcgagtgg gcaccgggac cactttccac gtctatttcc cagccgtcag
     3301 cgtgcggcag ccgagcccgc cggtcgagga tcgcgcccgc gggctggccg gcggcaaaga
     3361 aaccctgctc gtggtcgaag acgatccggc ggtgcgccgc gcgatcgtgg gcgtgctccg
     3421 acattgcggg tacaccgtgc tcgaagctgc gtccggacca gacgcgttag cactctgggc
     3481 ccagcgtcaa accgacatca agttgctgat taccgattgc atcatgccgc tcggaatcaa
     3541 cgggctcgat ctggcggatc ggctgcgtca cgatgaaccc tcgctcccgg tgctctacac
     3601 cagcggctac cgcgcgatgg gcatcgatag ctctcgccta gagcttggcg tcaactttct
     3661 cgagaagccg ttcgaagcca acgcgctcgc ggtgctcgtc cgcaagcaac tggacgtcag
     3721 cgtgatttga gccgcgcggg cggccgagcc tcaccgggct aacaattggg gacagaaact
     3781 gtccgcaatt cggagtagct tctgacccat gaaaaagacc taccgcggca gctgtcattg
     3841 caaacgagtc accttcgaag cagagttgga tctcagccag ggaacgggca agtgcaactg
     3901 ttcgtactgt tggaaggtca ggaactggag cgtgatcgtc aagccggacg cattccgcct
     3961 gctatcgggc gaagatcagc tgtccgacta tcagttcggc ggcaaggtgg ggcaccacct
     4021 gttctgcaag aactgcggag ttcgcacgtt tggccgcggc aacatcccag agatcggagg
     4081 cgagtactgc tccgtaatcc tgtccacgct cgacgatctg gatccgggcg agctggccga
     4141 agccccggtc cgctatttca acggccggga caatgactgg ttccacacgc cgtcggaaac
     4201 tcgacacctg tgagccctcg cccgcgccgc ggtccgcgcg aatcgagagc gccatatcga
     4261 gcatcgaggc tcgcgagcgg gcctctgcgg gtttctaaat ggacgcgatc tggcgcttga
     4321 tgccgccggc tgcgtgggcc gcattctgcc tcgctttggc gggtgtttac gcgcgcttct
     4381 ggccgcggcc gctgacacag gcaggccaga ccgagatgct cggttcaggg gggctgggcc
     4441 gagttcgcta cctagtgctg cgctggtttc attcgttggt ctggctgctg ctggcgctcg
     4501 cagtcgtatt gcgaggagaa gatcaacaca cctgggccgt gcgtgtcgcg acggctgcgc
     4561 tcgtcgtcta tgtcgttttc atcgtgtttt cgacgatggc gcggagacgt gaacgttccg
     4621 catccgggcc cgagtagagg cgagatcgca cttctggcgg gcgtccgcgt tctgcctctt
     4681 ttttaacttt tcgcccgaga tgcgctacgg gttggcaccg catcccgaga gagagtgtgc
     4741 gattccatct ggaaacactc cgcgggatct cgaacggagt agccatgacc cacaccgtga
     4801 accgtgacgc aatgtccatc gagccccacg cgatccgcgc acgcgcttac gaactttgga
     4861 acgagcgcgg gcgccccgac ggctccgccg accacgattg gctccgcgcc gaacaagagc
     4921 tcatgaaccg gctcagcgcc aagcagccgg ctgccaccgc gctccccgaa acgagcggcg
     4981 cgaccgtgag tacgccagtg gcgcaggatc cgacccaggc tcgagccgcc gtgaacaaga
     5041 ctgcggacaa acccagcgca agccgcgcca agaaatcgag ccgctccaaa gtgcgcgacg
     5101 ttagcgcgga acccggcgcg gccgcagcga cgcgattgct ggccaagttg gccaccaacg
     5161 cgcgcgcttg aagcttcggt cgagtccacg caggagcgct gcgccaactc gagaaccgaa
     5221 gcttagtaca gtcgctcagc gatcgggatc gggagcgagc atcccaatct tggcgtcata
     5281 ggtcatggcg ccaaactccg ggtgcttcac gacgatcgag tagtcgttgg gcaaatcgat
     5341 agccagcgtc ttcgggacgc gcggcatggg cgcaatgccc ttgtctttgc ccgacagctt
     5401 cagggtgatc tgatcagtgg tctcttcgcc gggggcgaat tgcaccttgt tccacttcaa
     5461 cagattgccc ttgtagcgat tgtacgtcac ccaccaccag gcgccgtgcg cgtctttggt
     5521 gaactggatc gattcgacgg ggactttggc gcgagcggcc tccaagcagg agcgaagcgc
     5581 agctcgatcg tcaccagcgc tttcttcgca gcgctgcttc gcgctctccc ctacttcgga
     5641 ttgagcgaag ttgaagacga agagcacgtc tggcgccatg atgagatcgt gaggaggacg
     5701 gcttgccttg cggatgccgt cgttttcgga gggaggcgga gccacagccg gggcggcttc
     5761 tttaggcgtc accgtgggtg tctcggcggc agcgtccttc gccgttggcg cggactcgga
     5821 gtggtcgccg gcgttcgatg caaccggagc gttagggtgg gccgtggtgg cagctgagcc
     5881 acaaccagcc acgagcgtag tcgcgatcgt gatgagagcg agggcagaca gagatgttcg
     5941 catgctgggc ctgattgccc gccgggtgga gcggcgagat caggggctac taacggcgaa
     6001 ggcgtgagct ttcgaacgtg cggatgcgcc acgacggcac ttttgtggga cgcacgtcac
     6061 tcgtcggctc actgccaccc cttgcgcggc tgttctcttt ccaaatacga acacctcggg
     6121 catcgagatg cctcgttgcg agccgctgaa ctccggcgag ttgcaatagg ctacttcatc
     6181 cctgggagtc tcatcctggt aaaagagaga tttgcccgcc gatgattgaa ttcacacacc
     6241 agcggtgact acaaggcgaa gcaactcagc tctcagtaca caggccgagg atcacggtcc
     6301 ccatggtgct cagcgtccgc gaattttcta gctggttagt gttgtttggt ttgctcggtg
     6361 cggcttgcga gcagggccgg gttacgggcg gacccgcttt cctgtccacc gactttactg
     6421 ccgaacagac ccaatcggtc gtcacggcgt tggatgaatg gcggcgcgcg accgctcatg
     6481 cggtggactt cactcccgta cttcggaacg aacgggacgg cgagcacgtg tcggtgcgac
     6541 cggggcgtct cggggagaat gaggccggcc gcgagtggcg cggacaaatc atgatcgatg
     6601 tagagctgct cgatcggcgt ggctcgggag aactcggggt gacgacggta accatgcacg
     6661 aacttggaca cgccattggc atccgcgctc atactagcga gggtttgatg cagtccgacg
     6721 tgtctttggc acacccttgt attgacgctt acactctcga gctggtctgt aattggatgg
     6781 cttgcgggcc cgaagccgcc ccaacttgcc cctgaaactc accctcgaac ggcgcgccct
     6841 ctcatcactc gaaacgaagg gcgtgggacg ggtccagctg cgcggcctga cgcgctggca
     6901 ttacgccgga tgtcgcgcca acgacgaacg ccgagcagaa cgccaggagc gcggtactac
     6961 ctgacaggcg gaccggaatc tgcggggccg aatacgtgac caccagcacc agaatgctgc
     7021 caaccgcgag accgattcca ccgcccagca acgcaatcaa tacgctctcg accaagaatt
     7081 gaaataggat atcaccacgg gttgcgccga cggcgcgacg gatcccgatc tctcgcgtgc
     7141 gctctctaac gctcacgagc atgatgttca tgacgcctac cccgccgacc aacaaggaaa
     7201 tcgaggcaat cgcgagcaag gcccaactca tggccgaggt aatggtctga aatacgcgca
     7261 gcaaatcatc ttgagtctgg atcgtgaaac agcgctcgcc acgccgtcga ccagcgagca
     7321 agcgatcgat ttgttgggtc gcgagctccg tctcgccgcc gctgcgcgtt gccgcgagga
     7381 tttgattcag gttggtctgt gcaaagaact ccgccgctac cgatacgggg atcaagccgt
     7441 gaccctgccc aagcccacgc ttggctcctg atctggttaa atgccattct gaattcgccc
     7501 gaagccggcc cttcggacca gcacgctcga gggcaactac cgtcatcctg acgtacgaat
     7561 gcccaactca cccacgcatc cgaaggggct gcggaatccc aggctcattt tggtctttgg
     7621 cccgcgcgca ttggcaacag ttcgtcacgt cccaccgcgc gccgcaggtt ttcgcgccca
     7681 cgcttgagtc gatctttcac tgtgttcggc gaggttcggg taatctcggc gatttcttcg
     7741 acggagtact cgaagccgca acgtagaatg agcgccgtac gctgccgctc cggtagtagg
     7801 tcgaggtact ggctcgcagc aattccggga tcggcgctgg cctggaccac cagcaaacaa
     7861 gcatcctccg tagaatcgaa gctatcgtgt cgcttctcac gaaactgacg gagcgtggtt
     7921 cgaatcgcga tccgatcggc ccaacgttcg aggctactct caccgcgaaa gttcgcggcc
     7981 gaccgcagaa tcgcaaacaa gctcgcctga ctagcgtctc gcgcatcctc ggcgtgtcgc
     8041 agcagtgatc tgcagagccg ctgaatgcga cgcatcaggc gcagcatgac gatgcgctgc
     8101 gccgccctct gcccggctgc cgcgaacctc atgagctcga tatccgtgtg ccttctctct
     8161 tcttcgccga cagtgcgttc tgccgaaacg tcgcgcgcgg gcccgacact catgtactct
     8221 cggtgtgagt cacgcctcga tcggggcgga gccagtgcca gttcgggttc tttaggtacg
     8281 acctccggtt ccggccgatg acgcccgccg ccaacacccg atttcgactc acccctacac
     8341 tccagacgag agcactcgcc agagtgattc ccattcgcgg accgctcgtt cctgccatga
     8401 atcacgtcga ctcgttcggg caccaaacaa gcgtcgaccg caaaatcgac tccacaatcc
     8461 agggccgcgc cggcgagaac attggcaaac caatcgccaa cgacgtcacc aacagagtca
     8521 tgcaaggttc aatggccctg ctctcgagca gggctgccgt ttggtggatc gggaccaccg
     8581 cggcggttct ctcgctgaca tccattcaac cgctgcgcgc cctggatgac tgggtgattg
     8641 cgctcgcggt aagcggtcgt ggcggcgaag ttggcctgcc ccggagcgcg tgggacgcct
     8701 ttacttttac acgcggtgac ccagccgaga acgcagcgct gagagatgcg gggctcatgc
     8761 tgccatggtg ggcagatacg caattacgcg tcgcgttctt tcgtcccttg gctgcactca
     8821 gccatgcctt ggacgaacgc ctttggccga atcaccccgc gctgatgcac gtgcacagtc
     8881 tggtgtggct cgtactgctc gttgcactca tgagccgagt ctaccgacgc tttgcggatt
     8941 cttcgggact ggccggattg gcgctggcgc tctacgcgct cgacgacgcg cggggtgcca
     9001 ccttggcctg gctctccaac cgcaacgcgc tgatgtgcgc ggtattcggc tgcggcgcgc
     9061 tcctggcgca cgatacgtgg cgccaaaaac gactcgccaa ggccgggatc ggcgccatag
     9121 cgttgttcct cgccacatgg gccagcggag aactcgcgat tggcgctctc gcctatctcg
     9181 ttgcctacgc cctcttcgtg gatcggggga gctggcgcgc gcgggccacc agcttaatgc
     9241 cctaccttct cctcagcctg atttgcgtgg tgaccacgcg ttccgcaggt ttcggcgtgc
     9301 agcgctcagg tgcctatctc gaaccctggt tcgatatctc tgccttcctt cagcaggcct
     9361 tcgaacgttg gctttggctg gccgcgagcc agctcggacc agtaccgtcc gatttgggtt
     9421 ggatgctcgg gccgggcgcg aagtcgtggt tatggttagg agccatctcc gtgatcggcc
     9481 tgtttgcatg gttgctgcgg ccattcctcg acgacgcgaa agttcgtttc tggctctgcg
     9541 gaggcatcct ctcactgcta ccgatggcca cgactctacc gagcgatcgt ctgttgacgt
     9601 tcgtggggat cggtgcttcc ccgttgctcg ctcgagtgct gggacccagc ctcaccgggc
     9661 cttcccgcag agtgcgaacc gcgcttgggt actctttcgc ggccgttcac ctcatcctcg
     9721 caccgctgct ctttccgttg cgggcggccc aaatgaattg gatcgcgagc gccgaggaga
     9781 ctgcgtatgc gtctctaggc gcgccgctgc ccgaggccaa aaccctaatt ttggttaacg
     9841 cgccccacgt cttggtgacc aactatatcc aagcccgctg gagctacctg aaggcctcgc
     9901 accccgcaca cgtccacagc ttggcggcga ccggtagcga cgtacgagtc acgcggcttg
     9961 gcgcgcagac gctgctggtc acaccacgct caggctactt cgacaccccg ttggagcgcg
    10021 cctatcgctc ggacttcacg gctctcgcgc gggcgcagtc agtccggttg acggctttcg
    10081 aggcccaggt cagcagcacg actccggatg gacgcccgcg ttccatagca tttagcttca
    10141 ctcgacccct gaacgaatac ctgtttgcgt gttgggtcgg cgggcgcttc gtgccctgcg
    10201 atctaccggc aattggtcga agtgccgtct tcgaagccga tgacctgaaa gtcgtggttc
    10261 gatcgctcac acggaactcc gaaccagact ccaaggcgcg ggaattcgaa atgaaaggct
    10321 cacggatcga atgaaagact cggagctcga gtgcgtggtg tgtgccagcg tgctgcgcgc
    10381 gacgcgagca gagagcacgc gcgtgaggag caacgtgcgc gcgttctctc acgaaagctt
    10441 cgaggtttgg cgctgtcgcg tttgctccag cattcacgcc cgagagcccg tggatcttgg
    10501 gcgctattac gccgaatatc cttttcacgg gttacgggag gaccttcgaa ctcgcatcgt
    10561 ctatgccaag caattggccc gattgcgccg cgccggcctc caacgttgcc accgcgtgct
    10621 ggattatggc tgcgggggag gccaattcgt acgctatctg gcgtctcgcg gttatgacag
    10681 ccacggcttc gacgcctact gcccgcaata ccagtctcgg tcgacgctcg aacggcagta
    10741 cgattgcgtg attacccagg acgtcatcga acacgtcgac tctcccaacg cgctattggg
    10801 agtactgaga tccctcacgc gaccggccgg cattctttgc gtcggcacac cgaatgccga
    10861 agcgatcgat cttcggcgcg cgcatgagtt cattcacacg ttgcacgcgc cgtatcatcg
    10921 gcacatcctt gggcgcgaag cgctcgtgca gcgcgcagag ctgcacgggt ttcagctccg
    10981 cgccttctac cgcaccatgt atacgaatac agcgctgttc ggcttgaatg aggcgttcta
    11041 ccgctactat gccggcctgc tcgacgacac gctggatgcc atcttgggcc ccatacaggt
    11101 cggccccatg ctgcgacgtc taccgctgac gctattctgg ggcttgtttg gcggcgcatt
    11161 ctcgcgaggg acagattgca ccgccatctt ctcgcgccag gactcaggac taacccagtc
    11221 cgcttgagtc ctggttgcgc tcgtgagctg cgtcagacgc ggctagcgcc gagcagcccc
    11281 acacgataac cgccgtcacg caagcgtcgc gaggccctac cgttcagcac aatcaacacc
    11341 acgagcgcca cgaagggaac gagcgtcaac gccgccaaga cgacgccgag cgtgctcgac
    11401 caccccagtc cgcgtgcaat ccggtatacc ccgatcacgg acaacaccac cgaagggaat
    11461 gccaacaagc cgacgattgg ccccatcgcg cggctcagtg cgtaagtcgc gatattcaac
    11521 agaatggcgt aaatgagcag tttttgacca ctcgcgagca cctccaaccg ctctgccgag
    11581 agcgcgccgc tcggagcgat cggtgaggcg acgtccgaac caggtaaatg cagattgtgc
    11641 tgggccattg ctcgctccct ccaggc
//
LOCUS       DASTKU010000692        16188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1248226, whole genome shotgun sequence.
ACCESSION   DASTKU010000692 DASTKU010000000
VERSION     DASTKU010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16188
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248226"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFQ61_24095"
     CDS             <1..516
                     /locus_tag="VFQ61_24095"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET9957609.1"
                     /translation="HPHADIRRGEAFLNLIYSVVTRGPAWRNTVLVVNYDEWGGFFDH
                     VPPPTAAIPDADRVAGNADGRLGFRTPALLVAPWARAGFVSHQVYDHTSVLKMIEWRW
                     GLEPLSVRDRDAANLADALDFAQPRPKPPLFAVPPGAPSLPCELTEALDEISLEHLRE
                     LALRHGWPVYD"
     gene            780..1370
                     /locus_tag="VFQ61_24100"
     CDS             780..1370
                     /locus_tag="VFQ61_24100"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET9957610.1"
                     /translation="MQGPPVLGRPVRSRILLVGQAPGDREPVLGRPFAWTAGKQLFKW
                     FATIGLDEEQFRSRVYMAAVCRCFPGKNPSGGDRVPAPQEIENCKPWLDAELMMLSPR
                     LVIPVGRVAIEQLLSAGPARAGLLTAYVGTQQRIRRANRTFDVIPLPHPSGASTWPRT
                     EPGKTLTAAALRLIADHSAWRKVLASPERTGGELGP"
     gene            1531..2496
                     /locus_tag="VFQ61_24105"
     CDS             1531..2496
                     /locus_tag="VFQ61_24105"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9957611.1"
                     /translation="MRRWPSTGEGYQGHAQRVAKSIVVKWVRPPDERTLARDPAALRS
                     HRRKLRSYAVEAAFYTRHSRRCDQTCRVPQAYVIENGERDWLFVLENLDAAGYAARRT
                     HASGADIRDALSWLAGFHARFLGVEPEGVWKRGSYWHLETRQDEWRALTDARVKEAAS
                     KIDERLARARFKTLIHGDAKLENFCFATGGVRPVQDGDSAARIAAVDFQYVGGGVGVQ
                     DVAYLLSSCLEGRELEAQAEQWLEFYLGELCRSAGQLRSTVGPLFSRAPVDEAALSEA
                     VEEWRALYPFAVADFQRFLLGWAPSHARDSYLIEVTERVLAAVQR"
     gene            2528..2653
                     /locus_tag="VFQ61_24110"
     CDS             2528..2653
                     /locus_tag="VFQ61_24110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957612.1"
                     /translation="MGLASCQPHGSCEHGVPGGAQMPQLGLQQTVSALQVVMPHI"
     gene            2669..2818
                     /locus_tag="VFQ61_24115"
     CDS             2669..2818
                     /locus_tag="VFQ61_24115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957613.1"
                     /translation="MAFVQGVVSQLAPGRTQVPQLGLQHSSPTLQVLTPHTTLSGMMG
                     MPHAV"
     gene            complement(3713..6397)
                     /locus_tag="VFQ61_24120"
     CDS             complement(3713..6397)
                     /locus_tag="VFQ61_24120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9957614.1"
                     /translation="MIAEPGEGAPNETVLAWRELLENTGGDLSRLPRLRGTRWPRRAG
                     VALPRLSSVYLDDALTARMVRRLERGETLFEALHHQLEDGLRCVRYAPLDVHEDCETR
                     VGQVITSLQQGGAERVALDLTRELPRLGVRARTYSVYSPTRASFPAPSATVDLPSLLP
                     AGANRAARVQRLAWELAGWGCDLVHAHLLDAEDLIGLSRYAFPTVVTVHNTRQGFPSG
                     LAELPASAASLLVACARAVENEIQSLGLSIPTRTVWNGVASPPEPTRSSSEFRERHGI
                     PAGALVLAALANPRPQKRLHLLPSIMVALDAHLEPTRPKPHLLMVGSSSGSSESARRA
                     QMELEGEVARCALQDRVHYLGATSHVHEVLAGSDVLVSPSEHEGLSLAHLEALSQGIP
                     VVATGSGGTAELAALCDGLTHLPLDFTPLEFAQAVLHVAQRPRAQSSQAVVARHFHAS
                     GMAAGYARLYARARAGAGTKRGDGVLLVTNNFSTGGAQSSARRLLCGMKAVGVRVRAA
                     VIEEQLEFPTPGRRALLDAGIPVLAIPPPVELEPGRAVEELLRTIDRDPPAAVLLWNV
                     IPEHKLLLVDGLWGVPVYDVSPGEMYFSSLERYFRKPRAGLPYLTARDYGSRLSGVIV
                     KYHAESQRAREVLGTEVYVIPNGIQSRQTHSNVNTTTRRSEPTTAITSGACAWSTARP
                     FTMVTATRLHPDKKLEDLLLALHLAAPRLPPHRLRIAGEVDGDCAGYAARLRELSIGL
                     RVEWVGECREVAELLGDADLFVLIAEPAGCPNASLEAMAFGLPILATDVGGMREQVID
                     GVNGCLVTRGSPREFAAALIELASSAERLRDMGRAGLNHARQTFSLEKMVRAYAEVCL
                     GRSACPDPADRGPAAPDPADPLPFDSSRDTRGADAAGS"
     gene            complement(6723..8345)
                     /locus_tag="VFQ61_24125"
     CDS             complement(6723..8345)
                     /locus_tag="VFQ61_24125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET9957615.1"
                     /translation="MRFGPVVGAWQGMREKRGPAGRAQKQAHGRAEPRPTREAKAAAA
                     EVHVEGQTLRPGARLARPLVAFLIVALGPSVPGLGHAQSATEVGAKAPLTPTLSIDLG
                     GLPLELALIRAGHFVQGSPLGETGRSEDETPHEVILTRDFYLARTPVTVAQFSRFTDE
                     TGYRTESEVGQSGGFGFDGTKLTQKPEFNWRHPGFPQTPDHPVTLVTFEDARAFTRWL
                     TEKTGRDFDLPSEAQWEYANRAGSALSYYAGGSASVAGLIGWFKSNSPNGTQPVARKP
                     SNAFGLYDMSGNVYEWCRDWYGPYPPSNVSDPELTSAPAGDKPRRVLRGGSWLKEAKH
                     LRAAARYRNDPRSRNADNGFRVLAAVNARVAPAPSSVSTASAVEDSKPAASSAGAKTL
                     LAMGVLATAAVSLIGVLTYLVRRSQRPRPMRGAPPGVNYRAQRDGFWLFAPPDLYGST
                     LHYRFFHRGAVRQAAVPIERGEQGQFVYTGATPDLVEAERIAGWGAAYAMAAAPRPVT
                     PRRGVGHAASEGEFSDSDPDSGSAFRGYPSAY"
     gene            8576..9427
                     /locus_tag="VFQ61_24130"
     CDS             8576..9427
                     /locus_tag="VFQ61_24130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9957616.1"
                     /translation="MNDIASNDMLETDSTLDELAFLNFTESLREFVRWGEGGVLSESE
                     DTLRTQTRVAFAAGSFNTAVFLGKRLDSPASWLETQRAWFHERGRGFSVHTRAAHHAE
                     LERACLQAGLRAEAKPPVMVRRAPLEPVPGTGAGATARVRLGNDLRDWKSFVEVTAAS
                     YVSMGFAEHLTRNLMNSPERMARPVWLCALARAGSTDCAAAALLFSHSIAGLYWVGTR
                     PTARRQGFAAACVQHLTNIAFERGARAVVLQASDAGAPLYRRMGFTDLTHYPMYVAAR
                     DVNVRDP"
     gene            complement(9494..10858)
                     /locus_tag="VFQ61_24135"
     CDS             complement(9494..10858)
                     /locus_tag="VFQ61_24135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9957617.1"
                     /translation="MQIIRDFGRGVTQQLARRAASARAIRWALLTLSIAGIQVDPGGT
                     AVARAAEPTREHANHERGTQWVAAWQGSATPGGTFYSEGCPSDIGLSDQTVRNLVRTS
                     VAGSAVRVRISNAGGSEPLKVGAASLAISSGDGGTLPGSLRPLRFAGKASTIVAAGAE
                     ALSDPVSLSVQALQTLALSVYLPESTGPATQHYFAAQDNFLAEGNQTNSPGAAPFSRK
                     ISCWMFVSGVEVRAPSRVLGSLVALGDSITDGYLSTTNANRRYPDLLAERFAARRGPT
                     LSVVNAGIIGNELLTVRPQLQFGYPVPARLARDVLTQAGAKAVILLAGINDIGDRSAK
                     AVDLIAVDQQIIQQVHAAGLRIYGATLVPFAGSTETYGGDYGTAAGEQERLKLNQWIR
                     SSGAFDGVVDFDRALRDPSDPSRLLTAYDADRLHPNDAGYQAMANTVDLDAILADVLR
                     DLSK"
     gene            11065..12192
                     /locus_tag="VFQ61_24140"
     CDS             11065..12192
                     /locus_tag="VFQ61_24140"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957618.1"
                     /translation="MITRITSHLRPPEPAAAPFCAVYRECGGCPLIGLSSAEQRAKKT
                     EQLMRTMHAQGVPCDAPAWVSDGRVWGYRNRLRLRFDQGRPVFFNANKDPSCSVLRPE
                     LRELIPRVAEWAGPRQAALSAFEHLEVRAPDVDGLGALAIYSSERVTRPRGTMGPLGL
                     QSSSHLTESEPLEAPPGMLLAQPGHPVPMQRFHWTVDIVNYVPVTSFMQVNSGVNDLL
                     IREIQELALSCGARTALDLYSGTGNFSLALLAAGLNVTSVEMDGLAFAGYRRALTERG
                     EQLSGAVCDVSEAANRLLDSGAAYDLVIANPPRAGLGKAAPRCASLSRTHFVLCCCRA
                     ESFARDARVLTQSGLQLVRLMAFDMFPGTEHLEVLGFFSRS"
     gene            12457..13152
                     /locus_tag="VFQ61_24145"
     CDS             12457..13152
                     /locus_tag="VFQ61_24145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957619.1"
                     /translation="MHDCIFCRAFGTLVAVEPIIPESLGLDQVTLEYQVCKGCQRYFG
                     QELEAFVLTQTPVALWRVKAIDLPPPAPSPRSCGVGCDCFLSVEELSSVSRFFLKIGL
                     ELVASLDAAGVRQARFDAARNYARFEMGEAWPLFYGVAEQLEGQRWFEPSESGVVECL
                     EYEYELLSVEDDCFLHFSMGGEHWLISLTQRYPELRLREMFPHLELNLLWHPTCPSLT
                     DGREQPAPSRRAS"
     gene            complement(13181..14584)
                     /locus_tag="VFQ61_24150"
     CDS             complement(13181..14584)
                     /locus_tag="VFQ61_24150"
                     /inference="COORDINATES: protein motif:HMM:NF012754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957620.1"
                     /translation="MIGLNSWNVCAFTLVATVACLATPKQDPEAVGGTCSNCKGGSGG
                     ALGGRAHGGTSNGGATTEGGASSGGTLVEGGADASEAGAGGEGATGGITGSGGAAAGS
                     GGDSGRAGGATDFVGGNGGEAGSTVVEPPYDCPKDAPLVGWAAVPGLEFTHALTGGGE
                     GTTVTATTAEELMDYAGSVEPHVILVSGTITVPALDVKSNKTIRGADGSAKLVGGIRV
                     IGTSIAAADMVSNVIIQNLSIDASGSNTSELMDEDDAITVAYAHHVWIDHVDTLDAGG
                     DSIDITNGSDYVTVSWTRFRFANAARRTGVRVGNSDANSAEDAGRLKVTLHHNWFSDR
                     VDQRMPRVRFGDVHVFNNYYSAVANKHCVTAGYESRLLVENNYFDGAVLPHVFFSFVD
                     NTSIFQEPTAEMVVNDNTYVGDSDLPEGKQSGQGLSFVPPYSEILDPADEKLKEDVRH
                     CAGPRPLEPQTPAPEDP"
     gene            14978..15634
                     /locus_tag="VFQ61_24155"
     CDS             14978..15634
                     /locus_tag="VFQ61_24155"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9957621.1"
                     /translation="MSVSPSGARSVRYASHISWQPSPSPHVRRKRFEHEGPAESGRVT
                     SLVEYAPGANFPAHDHPEGEEILVLEGTFSDERGDFSAGTYMLNPEGFRHAPFSQPGC
                     LLFVQLRKYAGPQRERVLIDTRTAPYVARMAGVSSLELYRSAHYPERHWLTKLEPNAQ
                     VGPIHLPKGEHIFVVSGTLEDEWGSYPRHTWLGLPPGFIHTPRSPGGCLLYIRQIDAG
                     "
     gene            15684..>16188
                     /locus_tag="VFQ61_24160"
     CDS             15684..>16188
                     /locus_tag="VFQ61_24160"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9957622.1"
                     /translation="MPAKAVQKLQRTLTVSRSFRIAVVADTHSAPHPNTCALLQEFRP
                     EAILHAGDIGDSSVLDTLGEVAPVLAIRGNIDVTARELPDCLTLDLVCEQTRLLRIFM
                     THIAVYGPKLRKEVLQQAKVEAAQLVVCGHSHVPFIGRDRGVSVFNPGSVGPRRFQLP
                     IVFGTMQL"
BASE COUNT         2866 a         5062 c         5124 g         3136 t
ORIGIN      
        1 cacccgcacg cggacatccg ccgcggcgag gcttttttga acctgatcta ttccgtggtg
       61 acgcgaggcc ccgcatggcg caacacggtc ctggtggtca actatgacga gtggggcggc
      121 tttttcgatc acgtcccccc accaaccgcg gccattccgg acgccgaccg cgtcgccggg
      181 aacgcagatg gtcggcttgg atttcgaact cctgccttgc tggtcgcgcc ttgggcacgc
      241 gcgggattcg tctcacacca ggtctatgat cacacgtcgg tgctcaaaat gatcgagtgg
      301 cgttgggggc tcgaaccgct gagtgttcga gatcgcgatg ccgcgaacct ggctgacgcg
      361 ctcgatttcg cgcagccacg gcccaagccg ccgttattcg cggttccacc cggagcgccg
      421 tcgctgccgt gcgagctgac cgaggctttg gacgaaatct ctctcgagca tctccgcgag
      481 ttggcgctcc gtcatggctg gcctgtatat gactgagcgc gggatgccta tgcaggcatt
      541 gcgcggcgca tcagcggagt aggtcccgga gcatgtgagg gtggccgtct ttcgtagggc
      601 gggtgctctc ctccggctgt actgtccgag cgttcgaaag cgtcgcgcaa cgttgacgcg
      661 cgggcggggc tacgcaacgc ctgggtttga ccttgcgctc attgcctctt ctgcagccgt
      721 ttccggaatc ggtccacgaa caccagcgcg tcgttcgaaa ttgccggcgc tgccaagcaa
      781 tgcaaggtcc cccggttttg ggcaggccgg tgcgctcgcg tattctcctg gtaggtcaag
      841 ctcctggtga tcgagagccg gtgctgggga gacccttcgc gtggaccgcc ggcaagcaac
      901 tattcaagtg gtttgcaacc atcgggctcg acgaagagca gttccggagt cgagtgtaca
      961 tggccgcagt gtgtcggtgc ttccctggta agaacccgag cggcggcgat cgcgttccgg
     1021 caccgcagga gatcgagaat tgcaagccgt ggctggatgc cgagctcatg atgctctcgc
     1081 ccaggctcgt gatcccagtt ggccgcgtcg caatcgagca gctcttgtca gccgggcccg
     1141 ctcgggcggg gctgctcacc gcctatgtgg gaacccaaca gcgcattcgg cgtgcgaata
     1201 ggacgttcga tgtcatcccg ctgccgcatc cgtcgggcgc ctcgacttgg ccgcgcaccg
     1261 agccgggcaa aactctgacc gcggcggcat tacgtctcat cgcggaccat tcggcgtggc
     1321 gcaaggtgct ggcatcgcca gagcggacgg ggggcgaact tggcccatga cgccagatcg
     1381 gatggaaagc acctcagctc tcgggtagcg caggccctgg gcgcacgaga actgcatctc
     1441 gagccccctt ttcaaagatt gtggggcggg tacggagacg cgcggcgggg cgtggtgctc
     1501 gagtcggctc gggtttccga ggacgaagat atgcgccggt ggccaagcac aggcgagggc
     1561 tatcaagggc atgcgcagcg ggtcgcgaag tccatcgtcg tcaagtgggt ccggcctcca
     1621 gacgagcgaa cgctcgctcg ggatcccgcc gcgctgcggt cgcatcgtcg gaagctgcgc
     1681 tcctacgcgg tggaggcggc gttctatacg cggcattcgc gacgctgtga ccagacgtgt
     1741 cgggtgccac aggcgtacgt catcgagaac ggtgaacggg actggttgtt cgtgctcgag
     1801 aatctggacg cggcaggcta tgccgcccgt cgcactcacg cgtcgggagc cgatattcga
     1861 gatgctctct cctggctcgc cggctttcac gcgagatttc tgggagtcga gcccgagggc
     1921 gtgtggaagc gaggcagcta ttggcatctg gagacacgcc aagatgagtg gcgcgcgctc
     1981 acagatgctc gggtcaagga ggctgcttcg aagatcgacg agcggctagc aagggcccgc
     2041 ttcaaaaccc tgattcacgg agatgccaag ctcgaaaact tctgctttgc gacgggcggt
     2101 gtgcggccgg tgcaagatgg tgactcggcc gcgcgcatcg ccgcagtgga tttccagtac
     2161 gtaggcggag gggtcggggt gcaagacgtc gcgtatctgc tgagcagttg cctggaaggg
     2221 cgcgaactcg aggcgcaggc tgagcagtgg ctcgagtttt acttgggtga gctgtgccgc
     2281 tccgcggggc agctccgctc gacagtgggt ccgctattct ctcgggcgcc cgtggatgag
     2341 gcggcgctga gtgaagccgt cgaggagtgg cgcgcgctat atcctttcgc ggtggccgac
     2401 tttcaacgct tcttattggg atgggcacct tcccacgccc gggattccta tctaatcgag
     2461 gtcaccgagc gagtgctcgc cgcggtgcaa agatgagtgg cacgctcaac gtcactactg
     2521 ggaggggatg ggcttagcct cgtgtcagcc gcatggctcg tgtgaacacg gggtgccggg
     2581 tggcgcgcag atgccgcagc tcgggttgca gcaaacggta tccgcgttgc aggtcgtcat
     2641 gccgcacatc tgacttacag ggttggtaat ggcgttcgta cagggtgtgg tgtcacagct
     2701 cgcaccgggc cggacgcaag tgccgcagct cggattgcag cacagttcgc ccacgttaca
     2761 ggtactcacg ccacacacga cgctctcggg catgatgggc atgccgcatg ccgtctgaga
     2821 gcatttttga ccaggatcga cgcaggtgcc gcagcttgcg ttgcagcaaa ccttgccgct
     2881 cgggcagacc gttttgccgc actgttcacc agccgcgggt gcgggcgcgc cctgaccagg
     2941 gccaccgccg atcccaggac tgccagtcgt cgtggctgga gggtttccgg ccgcgctact
     3001 cgcggcgacg gcgagtccat tcgaagttgg ctcagttgtt cccgtattgc cgacagattc
     3061 gctcggggcc acattccgcg cgacctctcg cgtagtcgtg gcctcatttg cggtgctgga
     3121 gccgaggtca gcgtcgttcg gtgcggctcc cggggcggat gagggcgcca ttgtaccggc
     3181 tgaagtgggc tgccattggg gcgcagcatc gggagggacg tcggggggag tgctcgctgc
     3241 accccgaggg ggtagcgtct gcgcggtgct cctgctgcca gtgttcgtct cagctgttcc
     3301 tggctcatcc gcggccctca tggcgagcac gtccgaggtg agctcgccgg cgtggatgct
     3361 cagtccgcga tatgcagtca ccagccaaac cgcgctcgtc gccaggagcc ccacgccggc
     3421 cgcgactcca atgaatgtgg gccaggtata ctttctacgc ttctgcgcga gagagagggg
     3481 cctcgttggg gcttgagctg ccggggcctc tcgagttgcc gttgtgggcc gtgcctcagc
     3541 ttcgttgatc ggcgccacca gcgtaacggg cagcggcgat gtcgagggat tcgagctcgg
     3601 taactccggg ttcgaggcaa tttccgcgtg ccccaggggt tccagcgttg cagagtctaa
     3661 aggggagggc gggagcgatt tagccacata cttcgcattg caagccctgg acctatgacc
     3721 ccgcagcgtc tgcgccgcga gtatctcgag aggagtcaaa gggcagcgga tcggcagggt
     3781 ccggagcagc agggcctcga tcagcagggt cgggacaagc agagcgccct agacaaacct
     3841 cggcatatgc cctcaccatc ttctctagcg agaaggtttg gcgcgcatgg ttcaaacccg
     3901 cacgtcccat gtctcgcagg cgttcggcgc tcgaggccag ctcgatgagc gcagcggcaa
     3961 actctcgtgg acttccccga gtgaccaagc aaccgttaac gccgtcgatc acctgctcgc
     4021 gcattccgcc cacgtcggtg gccaggatgg gcaagccgaa cgccatcgcc tcgagcgaag
     4081 cgtttgggca gcctgcgggc tccgcgatca gcacgaacag atccgcgtcg ccgaggagct
     4141 cagcaacttc gcggcattct ccgacccact cgactcgtag tcctatcgaa agctcgcgca
     4201 ggcgtgccgc gtagcccgcg cagtctccat cgacctcgcc cgcgatgcgc aaccggtgag
     4261 gcggtaggcg cggggccgcc aagtgtaggg ccaacagcag atcctccaac tttttgtcgg
     4321 ggtggagtcg cgtggcagtg accatcgtga aggggcgtgc cgtcgaccac gcgcatgcac
     4381 cactcgtgat cgcggtcgtt ggctcgctcc tcctcgtggt ggtgtttacg ttcgagtggg
     4441 tctgacgcga ttggatgcca ttgggaatga cgtaaacctc cgtccccagc acttcccgcg
     4501 cgcgctgaga ctcagcatgg tatttgacga tcaccccgga aaggcggctc ccgtagtctc
     4561 gcgccgtcaa gtagggcagg cccgcccgtg gttttcggaa gtagcgctcg agcgagctga
     4621 aatacatctc gccggggctc acgtcgtaca caggaacacc ccataggccg tcgacgagga
     4681 gcagtttgtg ttcggggatg acgttccaca acaacaccgc ggccggagga tctcgatcaa
     4741 tggttcgcag gagttcttcc acggcgcgac ctggctcgag ctccaccggt ggtgggatcg
     4801 cgaggaccgg aatcccagca tcgaggagcg cacgtcgacc tggcgtggga aactcgagct
     4861 gttcctcgat cacggccgct cgcactctca ccccaactgc tttcatgccg cacagcaggc
     4921 ggcgagcgct cgactgggcg ccacctgtcg aaaaattgtt ggtgacgagc aggactccgt
     4981 ccccgcgctt cgtgcccgct cccgcccgtg ctcgagcata aagccgcgcg tagcccgcgg
     5041 ccatgcctga agcatggaag tgccgcgcta ccacggcttg actcgactgg gctcgagggc
     5101 gctgggcaac gtgaagaacg gcttgcgcaa attcgagagg cgtgaagtcc aatggcaaat
     5161 gcgtgagccc gtcgcacagc gccgcgagct ccgcggttcc gccgctaccc gtcgcgacca
     5221 cggggattcc ttgcgagagc gcctccaggt gagccaggct caagccttcg tgttcgctcg
     5281 gggagaccag gacatcactg cccgccaaca cctcgtgcac gtgcgatgtg gcgccaagat
     5341 agtgcacgcg atcttgcaac gcgcagcgcg caacttctcc ttcgagctcc atttgcgcgc
     5401 ggcgcgcgct ctcactgctc ccggacgaac tgccgaccat caggaggtgc ggtttcggtc
     5461 gtgtcggttc gaggtgtgca tccagcgcga ccatgattga gggcagcagg tgtagcctct
     5521 tttgtgggcg aggattggct aaggccgcta acacgagcgc acctgcgggg atcccgtgcc
     5581 gctcacgaaa ctcagaggag gagcgtgtcg gttcgggcgg cgacgcaaca ccgttccata
     5641 cggtgcgtgt agggatggaa agtcccaaac tctggatctc gttctcgacg gctcgcgcac
     5701 acgcaacgag caggctcgcg gcggaggcgg ggagttcagc cagccccgac gggaagcctt
     5761 ggcgcgtatt gtgaaccgta accacggtcg gaaacgcgta acggctcagc ccaatcaggt
     5821 cttccgcgtc cagcagatgc gcgtgtacca aatcacagcc ccagcccgcg agttcccagg
     5881 cgagccgctg cacgcgtgcc gcacggtttg cgccagccgg gagcaggctg ggcaagtcca
     5941 ccgtagccga gggagcggga aaacttgcgc gagtaggcga atagaccgag taggtgcgag
     6001 ctcgaacccc gaggcgcggc aattcacgcg tgagatccag cgcgacccgc tctgcaccac
     6061 cctgttgtag actcgtgatt acttgaccaa cgcgggtttc acaatcttcg tgcacatcca
     6121 acggggcgta gcgcacgcaa cgtaatccat cctcgagttg gtgatgcagg gcttcgaaca
     6181 acgtctcgcc gcgttccaag cgccgcacca tgcgagccgt gagtgcatcg tcgagatata
     6241 cactcgacaa gcgtgggaga gccacgccgg ctcgtcgcgg ccaccgagtc ccacgcagcc
     6301 gtggcaagcg actcaggtcg cctccggtgt tctccagcag ctctcgccat gcgagcacgg
     6361 tctcgttcgg agcgccttct ccaggctctg caatcaaggc accgagggca cagatgggcc
     6421 gcccagtggc gctgggcgcg ggccacgtcg cagcttgccc gggtgcgctc ctgcgcaagc
     6481 aggccggcca cgcgccggct ctgaccagcc acagggcccc tgggcgtagt cgtcgagcta
     6541 gttcgctcca agctagctcg gaaagatccg atggctgcaa acctagctgg tttagagcgc
     6601 gcacggagcg cgcgcgttgc tcgcgttccg ccgagcactc ggagaagatc acgcattgaa
     6661 gtgcagtctg catcctgaac gccgaaggaa tcggagtatt cgaatcggaa gggcccggac
     6721 gatcagtagg ctgagggata gccgcggaac gcggacccgg aatcgggatc tgaatcgctg
     6781 aattcgccct cggaggctgc atggcctacg ccgcgtcttg gcgtgaccgg tcgtggcgct
     6841 gccgccatgg cgtacgcagc accccaacct gcgattcgct cggcttccac gagatcgggg
     6901 gtcgccccgg tgtagacgaa ctgaccttgc tcaccgcgct cgatgggtac cgcggcctgg
     6961 cgcaccgcac cgcgatgaaa gaaacggtag tgcagcgtag agccgtagag atccggcggt
     7021 gcaaacagcc agaaaccatc tcgctgggct cggtagttca ccccgggggg ggcgccacgc
     7081 atcgggcgtg gtcgttggct cctccgcacc aggtacgtaa gcacgccaat cagcgacaca
     7141 gcggccgtgg cgaggactcc cattgccagt agtgtcttcg ctcccgcgct ggaggcagcg
     7201 ggtttcgaat cttcaacggc gctcgccgtt gaaaccgagc ttggggcggg cgcgaccctg
     7261 gcattcaccg cggcgagcac gcggaaccca ttgtccgcgt tacggcttcg agggtcattg
     7321 cggtaacgcg cagccgcgcg cagatgcttt gcctctttca accaagagcc ccctcgtaga
     7381 acgcgacggg gcttgtcccc ggcgggagcg ctcgtgagct ccggatcact cacgttgctt
     7441 ggcgggtaag ggccatacca atcgcggcac cactcgtaga cgttgccgct catatcgtat
     7501 aagccgaaag cgttgctggg cttgcgggct acgggttgag tgccattcgg ggagttgctc
     7561 ttgaaccaac caatcagacc agccacgctc gcgctgccgc cggcatagta gctgagcgcg
     7621 cttcccgccc gattggcgta ctcccattgt gcttcactcg gtaagtcgaa atccctcccg
     7681 gtcttctcgg tcagccagcg cgtgaaggca cgcgcgtcct cgaacgtgac taatgtgacc
     7741 gggtgatcgg gggtttgcgg aaagcctgga tgccgccaat tgaactcggg cttttgggtg
     7801 agtttggttc cgtcgaaacc gaagcctccc gactgaccca cctccgattc tgtacggtag
     7861 ccggtctcgt cagtgaaacg cgagaactgc gcaaccgtaa cgggcgtgcg tgccaagtaa
     7921 aagtcgcgcg tcagtatgac ttcatgtggg gtctcgtctt cgctgcgacc ggtttctccc
     7981 aagggggagc cttgaacgaa atgcccggct cgaatcagcg caagttctag gggaaggccg
     8041 cccaggtcga tgctgagcgt aggggtgagg ggagctttgg ccccgacttc ggttgcggac
     8101 tgtgcgtggc ccaatcctgg cacgctcggg cccagcgcga cgatcagaaa ggctacgagc
     8161 ggacgggcca atcgcgctcc cggtcgcagt gtttggcctt cgacgtgaac ctcagccgct
     8221 gcggctttcg cctcccgagt cgggcgaggc tcggcgcgcc catgagcctg tttctgtgct
     8281 cgccctgcgg ggccgcgctt ttctcgcatg ccttgccaag cacctaccac cggaccgaat
     8341 cgcatttcga aactctaaca tcggccgctt tccgacgtgc cgccgatccg cacagcagtt
     8401 cgtgaaaaca cccaacccaa gggcccgttc aaccaccagc gccggcattg ctcgggccgc
     8461 ggctaggttt ataaaagagt gcgtcagtgc gtcgctgaac tcaccgcagg acgaatctga
     8521 tcgccccacg agcggagatt gaaacgatga gcggagattc agccgaggag cggagatgaa
     8581 cgacattgcg tcgaacgaca tgctcgagac ggactccacg ctcgatgagc tagcctttct
     8641 caacttcacc gagagcctgc gcgagttcgt ccgctggggt gagggagggg tgctgagcga
     8701 aagtgaggac accttgcgca ctcagacccg ggttgccttc gcggcgggaa gcttcaacac
     8761 cgccgtattt ctgggaaaac gcctcgattc gccagcgtcg tggctcgaaa cgcagcgcgc
     8821 ctggtttcac gaacggggtc ggggcttcag tgtgcacact cgtgccgcgc accacgccga
     8881 gctcgaacgc gcgtgcttgc aggcgggact gcgcgccgaa gcgaagccgc cggtgatggt
     8941 tcggcgtgct ccgctcgagc ccgttcccgg caccggcgct ggtgcgaccg ctcgcgtgcg
     9001 actcgggaac gacctccgcg attggaagtc cttcgtcgag gtgaccgcgg cctcctacgt
     9061 cagcatgggt tttgccgaac atctgactcg caatctgatg aattcaccgg agcgcatggc
     9121 gcgacccgtg tggttgtgcg ctttggcacg cgcagggagc accgattgcg cggcggcggc
     9181 cttgctcttc agtcattcca tagctggctt gtactgggtc ggcactcgcc cgacggcccg
     9241 gcgccagggg tttgctgccg cctgcgtgca gcacctcacg aacatcgcat tcgaacgcgg
     9301 cgctcgcgcc gtggtgctgc aggcgagcga cgctggtgct ccgctttatc gccggatggg
     9361 gttcactgat ctcactcact atcccatgta cgtggcggca agggacgtaa atgtgcgcga
     9421 tccgtgaacc cacagcacga gcgtcgaaaa tgccggaaag tcgtgcttcg agccgagcta
     9481 atcgacactc gaactatttc gatagatcac gcaacacgtc cgccaggatc gcatcgagat
     9541 ccaccgtgtt tgccatggct tgatatccag cgtcgttcgg atgcagacga tctgcgtcgt
     9601 aggcagtcaa gagccgactc ggatcggaag gatctcgcag cgcgcgatcg aagtccacca
     9661 cgccatcgaa ggccccgctc gagcgaatcc actggttcag cttcaaccgt tcctgttccc
     9721 cggccgcggt tccgtagtcg ccgccgtagg tctcggtcga gcccgcgaat ggcaccaagg
     9781 tggcaccgta gatcctcaat cccgcggcat ggacctgctg aatgatttgc tgatccaccg
     9841 cgatcaagtc aaccgccttc gcggaacgat cgccgatgtc gttgattccc gccaacagga
     9901 tgaccgcctt ggcgcccgct tgcgtcaaaa cgtcacgtgc taggcgtgcg ggcaccgggt
     9961 agccaaactg aagctgtggc ctcaccgtca gcagctcatt gccgatgatc cctgcgttca
    10021 ccactgagag ggtggggccc cggcgtgccg caaatcgctc ggcgagcaga tcgggataac
    10081 gccggttcgc gttggtggtc gacaggtaac cgtcagtaat cgaatcaccg agcgcgacta
    10141 gcgagcccaa cactcgagac ggggctcgca cttccacgcc cgaaacgaac atccagcacg
    10201 aaatttttcg tgaaaacggg gccgctccag ggctattcgt ttggttgccc tcggccagga
    10261 agttgtcttg ggccgcgaaa tagtgctgcg tggcggggcc ggtgctttcc ggaaggtaaa
    10321 cgctcagcgc cagcgtctgc agcgcttgta cggacaagga caccggatcg ctgagagctt
    10381 ctgcgccggc cgccacgatg gtcgaagctt tccccgcgaa ccgcaatggg cggaggctac
    10441 cgggtagcgt gccgccatct cccgaagaaa tagcgagaga agcggcgcct actttcaacg
    10501 gctcactgcc cccggcattt gaaatacgca cgcgcaccgc actccccgca acactggtcc
    10561 gaaccaggtt tcttacggtc tgatcgctca gtccgatatc cgatgggcag ccctccgagt
    10621 agaaggttcc tcccggggtg gcgctgcctt gccaggccgc cacccattgc gtgccgcgct
    10681 catggtttgc atgctctcgt gtaggctccg ctgctcgggc cacggcggtt cctcctgggt
    10741 cgacctggat ccctgcaatc gaaagcgtta gcagcgccca acgtatggcg cgagcgctcg
    10801 ctgctcttcg agctagctgc tgtgtcacgc cacgtccgaa gtcacgtatg atctgcatcc
    10861 tctcctcctg ctttccggtc gacagcttgc acgtcccgcc ggtcctatgg catctcgttt
    10921 tcggctagag ctgtggccac ctcttgccca aatgcctatt caagtcgagc gagccagctt
    10981 ctcttggacc gagctgacct gtagaagctc caactgctcg atacagccca tggaggccgg
    11041 ccgcaagagc gcgcaccact gaccatgatc actagaatca ccagtcatct ccgtccgccg
    11101 gagccggcgg ccgcgccatt ttgcgcggtg tatcgtgagt gtgggggctg tcccctcatt
    11161 ggcctgtcga gcgcggagca gcgtgcgaag aagaccgaac agctgatgcg gacaatgcac
    11221 gctcagggcg tgccgtgcga tgctccagcg tgggtgagcg acggccgggt gtggggctac
    11281 cgaaatcgcc ttcgcttacg cttcgaccaa ggtaggcccg tcttcttcaa cgccaacaag
    11341 gacccaagtt gtagcgtgtt gcgcccagag ctgcgtgagc tcatcccgcg ggtagcagaa
    11401 tgggcaggcc cgcggcaggc agctctctct gcattcgaac acctagaagt gcgcgcaccc
    11461 gacgtcgatg ggctcggtgc gctggccata tactcttccg aacgagtgac tcgcccgcgt
    11521 ggcacgatgg gcccactcgg cctgcaatcg agctcgcatc taactgagtc cgagccgctc
    11581 gaagcgccgc cggggatgct gcttgctcaa ccagggcacc cggtgcccat gcagcgcttt
    11641 cattggacgg tggatatcgt gaactatgtc cccgtgacct ccttcatgca ggtcaacagc
    11701 ggcgtgaatg acttgctgat ccgtgagatc caggaactcg cgctgagttg cggcgcccgc
    11761 accgccctcg acctctattc aggcactggc aacttcagct tggcactgct cgccgcaggc
    11821 ctgaacgtca cttccgtcga gatggatggc ttggctttcg cggggtatcg tcgcgcattg
    11881 acagaacgag gcgagcaact gagcggggca gtttgcgacg tgagtgaggc cgccaatagg
    11941 cttctcgact caggggcggc gtatgatctg gtaatcgcga atccccccag ggctggtttg
    12001 gggaaagccg ccccacggtg cgcgtcactg tctcgcacgc attttgttct gtgctgttgc
    12061 cgtgcagaaa gctttgcccg cgatgcccgc gtcttgacgc aatcggggct gcaactggtc
    12121 cggttaatgg cgttcgatat gttccccggt accgagcacc tcgaggtgct cgggttcttc
    12181 agtcggtcgt gagcaagggc tagacacgtc cgagtttcac caaccttcgt gaacaaactt
    12241 ctcaaaagca gagtgcaaga cagagcccgt tacgcccttt tcgaaatctg cgattgtgag
    12301 tgaccactct gcaaagtaga agggtgtgcg tgcgcggtcc ccgtctttgc gccgcggaga
    12361 tgccatgagt cgtgcgcaaa atgcccctgc ccaaatgcgt tggctggatg ccgatcgggc
    12421 gtggacaagc gagactcgaa gcttcagcct tcaattgtgc atgattgtat tttttgtcgg
    12481 gccttcggaa ctctcgttgc ggtcgaaccg atcattcccg agtctctcgg gctcgatcag
    12541 gtcacgttgg agtatcaagt ctgtaaggga tgtcagcgct acttcggcca agagctcgaa
    12601 gctttcgtgc tgacgcaaac cccggtcgca ctttggcggg tcaaggctat cgacctgccg
    12661 cctccggcgc cctcaccgcg ctcgtgcggt gttggttgtg actgcttctt gagcgtcgag
    12721 gagctgagct cggtgagccg gttctttctg aaaatcgggc tcgaactggt ggcgagtttg
    12781 gacgcggccg gggtgcgcca agctcgtttc gacgccgctc ggaactacgc ccgtttcgag
    12841 atgggagaag cctggccact attctacggg gtggccgagc aactcgaggg acagcgctgg
    12901 ttcgagccca gcgagtccgg agtggtcgag tgcctcgaat acgaatacga gctgctctcg
    12961 gtcgaggacg actgctttct gcatttcagc atgggcgggg agcattggtt gatttcgctg
    13021 acccagcgtt acccagagct gcggttgcgc gaaatgtttc cccatctcga gctgaatctg
    13081 ctgtggcatc ccacttgtcc aagtctcacg gacggccgcg agcagcccgc gccttccaga
    13141 cgcgcgagct gaccactaaa cgattcgcag ggcgaagcag tcagggatct tcgggcgctg
    13201 gtgtctgggg ctcgaggggt ctcggacccg cgcaatggcg cacgtcttct ttgagcttct
    13261 cgtctgcggg gtcgaggatc tcggagtagg ggggcacgaa gctcaggcct tggccggatt
    13321 gctttccttc tggaaggtca gaatcgccga cgtaggtgtt gtcgttgacc accatctcgg
    13381 cggtgggctc ctggaaaatg ctcgtgttgt cgacgaacga gaagaacacg tgggggagaa
    13441 ccgcgccgtc gaaatagttg ttctcgacga ggagccggga ctcgtagccg gcggtgacgc
    13501 agtgcttgtt tgctacggcg gagtagtagt tgttgaagac atgcacgtct ccgaagcgaa
    13561 cgcgcggcat gcgctgatcc accctgtccg aaaaccaatt gtggtgcagc gtaaccttga
    13621 gccttcccgc atcttcagcg ctgttggcgt cgctgttgcc gacgcgaacg ccggtacgcc
    13681 tcgccgcgtt cgcgaaccga aatcgcgtcc atgagacggt tacgtagtcc gagccattcg
    13741 tgatatcgat gctgtcacct ccggcgtcca gcgtatcgac gtggtcaatc caaacatggt
    13801 gcgcgtaggc gaccgtgatc gcatcgtcct cgtccatcag ttcggaggta ttcgaaccag
    13861 acgcatcgat tgaaagattc tggatgatta cgttggacac catgtctgcc gctgcgatcg
    13921 acgtgcctat cacgcgaatc ccgccgacca gcttggcact tccatcggcc ccgcgaatgg
    13981 tcttgttgga cttcacatcg agcgcaggta ccgtgatcgt gcccgagacc aagatgacgt
    14041 gtggttcgac gctgcccgca tagtccatca gctcctccgc ggtggttgcg gtgaccgtgg
    14101 tgccctctcc gccaccagtc agggcgtgcg taaactccag gccgggcacc gcggcccagc
    14161 ccaccaacgg cgcatctttt gggcaatcgt agggtggttc cacaacggtt gagcccgctt
    14221 caccaccatt gccgccgacg aaatccgtag cgccaccggc ccgtcccgag tctcccccgg
    14281 atcccgcggc ggcgcctccg ctgccagtga tgccccctgt tgctccttcc ccgcccgcac
    14341 cggcttcact cgcatcagct cccccttcca ctagtgtgcc gccagacgag gctccgcctt
    14401 ccgttgttgc gccgccgttg ctggtgccgc cgtgggccct tccgccgagc gctcctccgg
    14461 agcctccttt gcagttgctg caagtgcctc ctaccgcttc gggatcttgt ttgggcgtgg
    14521 ccaaacacgc caccgtcgcc accagtgtga aagcgcaaac gttccaggaa ttcaaaccaa
    14581 tcatcgtcaa cgttttcctc gcatcccgtg tgggcccgac gggttgccga aagaagccag
    14641 ccgaggcgcg tggcgacggt ttagtggtgc agggctctcg gatctcccaa tcttcgagcg
    14701 gggccgctcc aaccgggaga atacggttat tcttcgagag atgaagaccc ttcttcgcgt
    14761 tcagcgacat ttggcgtgtt ctgagtcata ggtcgagcgc gagtccattt tcgtaaatac
    14821 aactctgtca gcgacaacgc tgtcagcgcg tcggtcgtga gatgtaggcg cgtgtcgagc
    14881 ggctgcgtcc gccccggatc tcgagctcga gtctggggca cgcgaggaga acgccaacgc
    14941 cactgcgttt ccaagtgggc tcaggcatac tgaggaaatg tcagtgtcgc cctccggagc
    15001 caggagcgtt cggtacgcct cccacatatc ctggcagcca agcccatccc ctcacgtacg
    15061 ccgcaaacgc ttcgagcacg aaggccccgc cgagtcgggc cgagtcacct cattggtgga
    15121 gtacgcgccc ggcgccaact ttcccgccca cgaccatcca gaaggggagg agatcttagt
    15181 tctcgaagga accttttccg acgaacgcgg cgacttctcg gctggcacct acatgctgaa
    15241 tccggaaggt ttccgtcacg cacccttttc gcagccaggt tgtctgctgt tcgttcaact
    15301 ccgaaagtac gctgggccgc aacgtgagag agtgctgatc gatacgcgta ccgccccgta
    15361 cgtggcgcgc atggctgggg tgagctctct ggagctgtac cggtcagcac actacccgga
    15421 gcgccactgg ctgaccaagc tcgagcccaa tgctcaagtg gggcccattc atcttccgaa
    15481 gggcgagcat atcttcgtgg tcagcggcac cctggaggac gagtggggca gctatccccg
    15541 gcatacttgg ctcggcttgc ctccaggctt catccacacc ccgcgtagcc ccggcggctg
    15601 tcttctttac attcgccaga tcgacgccgg ttgagcagac ccgccttgct cgaaccattt
    15661 ccttgggtac tgtgctttgc gacatgcccg ccaaggccgt tcagaagctt cagcgtacac
    15721 tgaccgtgag ccgctcgttc cgtatcgccg tcgtagcgga tacgcactcg gctccgcatc
    15781 caaacacctg cgcgttgctc caggagtttc gtcccgaggc gatccttcac gcgggagaca
    15841 ttggcgactc gagcgtgctc gatacgctcg gcgaagtggc gccggtcttg gccattcgtg
    15901 gcaatatcga tgttaccgcg cgggagctgc cggactgttt gactctggat ttagtatgcg
    15961 aacaaactcg gctgctgcga atcttcatga cccacattgc agtgtacggt cccaagcttc
    16021 gcaaggaggt cctgcagcag gccaaagtcg aggccgccca gctcgtggta tgcggacact
    16081 ctcacgtccc cttcatcggg cgggatcgtg gggtttctgt gttcaacccc ggatcggtgg
    16141 gcccccgacg ctttcagctc ccgattgtgt ttggaaccat gcagttga
//
LOCUS       DASTKU010000693         4825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_355140, whole genome shotgun sequence.
ACCESSION   DASTKU010000693 DASTKU010000000
VERSION     DASTKU010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355140"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VFQ61_24165"
     CDS             <1..521
                     /locus_tag="VFQ61_24165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957623.1"
                     /translation="FGCTSTGCNSTGGTSFGCTSTGCNSTGGTVIGTTMGGSTADAGS
                     ARGAGAGQGGDSEVDCTTSRPFGQCTGSCNKTVAEYCEKYTSCDLTPEERCQRIWFGA
                     TWQRGCGYISAEYRGDVGDSGMDIWEESTGKLVYHWFNGNLSVGCVPQTIVGEKPSCA
                     EWAPACPIDEGS"
     gene            647..1402
                     /locus_tag="VFQ61_24170"
     CDS             647..1402
                     /locus_tag="VFQ61_24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9957624.1"
                     /translation="MHTAGRRGATRVVSFSRVRTPASSLDDPTVYPVEEPVGEDILQC
                     WMLEVLRPLVERWLAERGAPSFVGSDQFIYFEKGQPAQRIAPDLYILPGVAPDTHIPS
                     WKLWETGIRPSFALEICSLDWRKDYVTAPLLHDQIGTQELIVVDPDHLKRPEGYRFQR
                     FERTSRGLERQERTQADRVRSRILGCFLRLVGTEQSSRQRVRLALDPEGDVLFPTHEE
                     RVEAERQRADAERQRADAALARVAELEALLEQR"
     gene            complement(1623..2558)
                     /locus_tag="VFQ61_24175"
     CDS             complement(1623..2558)
                     /locus_tag="VFQ61_24175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957625.1"
                     /translation="MKSMLLRNSLALISLASLVACGETLDPGSRVTSFRVLAQQMDSP
                     YAAPGETVHVTSLVADPQSRPIHWAWAMCVNPISSEVGGCMDKIQADSEAKGSALLIA
                     EGVGVDAVDVTVPSDALSSLPESARSQASVGVLSVACPGTLSIASGASELPFRCVETG
                     TGRELGLHEFVVGVKRIVVRDRDRNLNPSIARVTFDGMEWPETEVKRAAACHETGNDF
                     SCPDSLQHKIAAEPTPQSIETGVSEFSQDFSEQVVVQYYASEGTFENETRIASNPETK
                     WAPRDPATGQDLTFWFVVRDNRGGVTWTTRQLHVE"
     gene            complement(2555..>4825)
                     /locus_tag="VFQ61_24180"
     CDS             complement(2555..>4825)
                     /locus_tag="VFQ61_24180"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9957626.1"
                     /translation="VEAPEPVYPEDKKSSGESARVGLLLTVDETGHVSQAEVSASAGP
                     EFDQAALAAARGLVFKPATRDGKASPARIPFHFDFTFQAPPPPEPAAAPAVAPPPAPP
                     AAIEPSAEAADSLDIDVEGDKPPREPTQRTLLVEEITKLPGTNGDALRSLTNMPGVAR
                     PAAFDGLLIVRGSAPQDTQVFVDGTSVPLIYHLGGLSSVVPSEILERIDFYPGNFGPQ
                     YGRSMGGIVDVGVRSPRKDRFHGLLQFDSVDGRFLAEGPITDSTRVLVAGRRSWLDAW
                     LGPALRSTGVGVTVAPVYYDYQAMIEHDISRNTQLRLFAFGADDRTELTLNSPDSQDP
                     ALGGSVLSREAFWRVQARLDTRPTDSLRWTTTLSAGHNSERQTVGDLFVDVDVLSFEG
                     RSDLRAKLSPQLTAVAGFDVQHNYFDAKVRLPPIEFGSDEPSGPLFGRPMNTLKASGG
                     VLRPSAYAMLEVAPLPGLKIFPGVRTDYNQDTGDWTVDPRVGVRYDVHPSFPRTTLKG
                     GVGIFHQPPEPYETAKEFGTSKLDSNIATHYSLGFEQEFLPPLELSVEGFYKDLRKLV
                     ISSPSATSNAAGQTYSNTGTGRAYGAEVLLRYKPSGPFFGWLAYTLSRSERRDAADDA
                     KYLFDYDQTHILTALASYKLGRGWQVGARFRYVSGKPYTPNLGGVMDYDAGVYSPVAS
                     PRQNSARLPAFQQLDVRVDKTWKFKSWSLAWYLDIQNAYNHKNVEAQGDNYDYSATTK
                     IYGLPILPIVGLRGEL"
BASE COUNT          875 a         1414 c         1603 g          933 t
ORIGIN      
        1 cgtttggatg cacctcgact ggatgcaact cgaccggagg tacttcgttt ggatgcacct
       61 cgactggatg caactcgacc ggaggtaccg tcatcggcac cacgatgggc ggaagcactg
      121 cagatgccgg gtctgctcgg ggcgctggcg caggacaggg cggagacagc gaagttgatt
      181 gcaccacctc gagaccgttt gggcaatgca cgggcagctg caataagacc gtcgccgagt
      241 actgtgagaa gtacaccagt tgcgatctga cccctgagga gcggtgtcaa aggatctggt
      301 ttggcgcaac ttggcagcgc ggctgcggct acatcagcgc cgagtatcgt ggagacgtgg
      361 gcgattccgg catggacatt tgggaagaat cgaccggaaa gctcgtctat cactggttca
      421 acggcaatct ctcggtcggc tgcgttcccc aaacgatcgt tggcgagaag cctagttgtg
      481 cagagtgggc gcctgcttgt ccgattgacg agggttcttg agtatcgcgg cactcgggat
      541 atcccgagtg ccgtcgttcc gaactccggg atttgcccgc attcctgcct agtttttctg
      601 ccattttgtt caggcgttct cgctgcctcg tgctagatgc cgatgcatgc acactgccgg
      661 tcggcggggc gccacgcgcg tggtatcgtt tagtcgcgtg cgcactccag cctcttctct
      721 cgatgatccg acggtgtatc ccgtggagga gcccgtgggg gaagacatct tgcaatgctg
      781 gatgctcgaa gtgctcagac ccctcgtcga gcggtggctc gcagagcgcg gcgccccctc
      841 tttcgtcggt tccgaccagt tcatctactt cgagaagggt cagcccgccc agcgcatcgc
      901 acccgatctc tacatcttgc ccggggtcgc gccagacacc catatcccct cttggaagct
      961 ctgggagacc ggcattcgtc cgagcttcgc cttggagatc tgctcgttgg attggcgcaa
     1021 ggactacgtc accgctccgc tgctccacga ccaaatcgga actcaagagc tcatcgtggt
     1081 cgatccggat cacctaaaaa gacccgaggg ttatcgcttc cagcgtttcg aacgcacatc
     1141 acgcgggctc gaacgccaag aacgcaccca ggcggaccgc gtccgctccc gtatactcgg
     1201 gtgtttcctg cgcctggtcg gcaccgagca atcctctcga cagcgcgtgc gactcgcgct
     1261 cgacccagag ggtgacgtcc tgtttcctac tcacgaggag cgcgtcgaag ctgaacgaca
     1321 gcgcgccgat gctgaacgac aacgcgccga tgctgcgcta gctcgcgttg ccgagctcga
     1381 ggcgttgctt gaacagcgtt agaggggcgc cctcgcggca atgcgagggc agcacgctct
     1441 tgctgggtcg aaacatccgt gaagcctggt agcccaggtc gaattgcccc agctggttga
     1501 aacaggacga cgggtggagg cccgcttgtt tggcccgact gaccacctgg attgccacga
     1561 tcgaaatccg aacgctcgcg ttgcgcgccg ggcgtttcga ggcgcgccgg cgcagagctc
     1621 cgctattcga cgtgcagctg gcgcgtggtc caggtgacgc cgccgcggtt gtcgcgcacg
     1681 acgaaccaga aggtgaggtc ttggcccgtg gcggggtcgc gaggggccca cttggtttcg
     1741 gggttgctcg cgatgcgcgt ctcgttttcg aacgtgcctt cgctagcgta gtattgaacg
     1801 acgacttgtt ccgagaagtc ttgggagaac tcgctgactc cagtttcgat gctttgcgga
     1861 gtcggctcgg cggcaatctt gtgttgtagg ctgtcggggc aggagaagtc gttgcctgtc
     1921 tcgtggcaag cagccgctcg tttcacttcg gtctcgggcc actccatgcc gtcgaaggtg
     1981 acacgcgcaa tgctggggtt gaggttgcga tcgcggtcgc gaacgacgat gcgtttgacg
     2041 ccaacgacga actcatggag gccgagttcg cgaccggtgc cggtctcgac gcagcggaag
     2101 ggcaactcgg aggctccgct cgcgatgctc aaggtgcccg gacaagcgac gctgagcact
     2161 ccgacggagg cttgagatcg cgctgactcc ggcagggagc tcaatgcgtc gctcggaacg
     2221 gtgacgtcga cggcgtcgac cccgacaccc tcggcaatga ggagggcact gcctttggcc
     2281 tcgctatcgg cttggatctt atccatgcag ccgccgacct cactggagat gggattgacg
     2341 cacatggccc acgcccagtg aatcggacgt gattgtgggt ccgccacgag tgaggtgacg
     2401 tgaacggtct cgcccggcgc ggcgtagggg gagtccatct gctgggcgag cactcgaaaa
     2461 gaggtgacgc gcgagcctgg gtcgagtgtt tccccacacg cgacgagaga cgccaaagaa
     2521 atcagggcca atgaattacg caaaagcatg gatttcatag ctcgcctcgc aaaccgacga
     2581 tgggcaggat gggcaatccg tagatcttcg tggttgccga ataatcgtag ttgtcgcctt
     2641 gggcttctac gttcttgtgg ttgtaagcgt tctgaatgtc gagataccag gccaatgacc
     2701 agctcttgaa tttccaggtt ttgtccacgc gcacgtccaa ttgctgaaaa gccggcaagc
     2761 gtgccgagtt ttgacgcggg gaagcgacgg gcgagtagac gccagcgtcg tagtccatga
     2821 ctccgccgag attgggcgtg tagggcttgc ccgagacgta gcggaaacgc gcgcccactt
     2881 gccagccacg acccagtttg tagctggcga gcgcggtgag aatgtgggtt tggtcgtagt
     2941 cgaacaggta cttggcgtcg tcggccgcat cgcgccgttc actgcgtgac aaggtataag
     3001 cgagccagcc gaagaatggg ccactcggct tgtagcgcag tagtacctcg gctccgtatg
     3061 cgcggcccgt gccagtgttg gagtaggttt ggcccgcggc gttggaagtc gcggaagggc
     3121 tcgagatcac gagtttccgc aaatccttgt aaaagccttc caccgagagc tcgagcgggg
     3181 gcaggaattc ctgctcgaaa cccaagctgt agtgggtggc gatgttgcta tcgagcttcg
     3241 aggttccgaa ctccttggcg gtttcgtacg gctcgggcgg ttggtgaaaa atcccgacac
     3301 cacccttgag ggtcgtgcgc ggaaagctcg ggtgcacatc gtaacgcacg ccgacccgtg
     3361 gatccacggt ccaatcaccg gtgtcctgat tgtagtcggt gcgcaccccg gggaaaatct
     3421 tgagaccggg tagcggggcg acttcgagca tggcgtaggc cgagggtcgc agcacgccgc
     3481 ccgatgcttt gagcgtgttc atggggcggc cgaataaagg gcccgatggc tcgtccgagc
     3541 cgaactcgat gggtggcaat ctgactttgg catcgaagta gttgtgctgg acgtcgaagc
     3601 ccgcgacggc ggtgagctgt gggctgagct tggcgcgtag gtccgaccga ccttcgaagc
     3661 tgagcacgtc cacgtctacg aagaggtccc cgaccgtctg ccgctcgctg ttgtgaccag
     3721 cggacaacgt ggtcgtccag cgtagcgaat cggtaggacg agtatcgagg cgggcctgaa
     3781 cgcgccagaa ggcttctcga ctcagcaccg atccaccgag ggccggatct tgggaatcgg
     3841 gtgagttcag ggtcaactcc gtgcgatcgt cggccccgaa agcgaaaaga cggagctgcg
     3901 tgttgcgcga gatgtcgtgc tcgatcatcg cttgatagtc gtaataaacg ggcgcgaccg
     3961 taacgcccac gccggtgctg cgcagggcgg gcccgagcca tgcgtcgagc cacgaacgtc
     4021 ggcccgcgac cagcacgcgc gtggaatccg taatcgggcc ttcggcgagg aagcggccat
     4081 ctaccgagtc gaattggagc aggccatgga agcggtcttt gcgcggggaa cgcacgccca
     4141 cgtccacgat gccgcccatg gagcgaccat attgagggcc gaagttgccg gggtagaagt
     4201 cgatgcgctc cagaatctcg ctcggcacga cggaggagag gccgcccaag tggtaaatca
     4261 acggcacgct cgtgccgtcg acgaagactt gagtgtcttg cggtgcggag ccgcgcacga
     4321 tcagcaaacc gtcgaacgca gcggggcgag ccacacccgg catgttggtc agagagcgca
     4381 gcgcgtctcc attggtgccg ggtagcttcg tgatttcttc gacgagcagg gtacgctgcg
     4441 tgggctcgcg cggcggcttg tcgccctcga cgtcgatatc caaggagtcg gccgcttcgg
     4501 cgctgggctc gattgcggct ggcggcgcgg gcggcggcgc caccgccggc gccgccgcgg
     4561 gttcgggggg cggcggcgcc tggaaagtga agtcgaagtg aaagggaatt cgcgcggggc
     4621 tcgccttgcc gtcgcgcgtt gcgggcttga atacgagacc gcgcgcggcg gccaacgccg
     4681 cttgatcgaa ttcggggcct gccgaggcgc tgacctcggc ttggctcacg tgcccggttt
     4741 catcgaccgt caacagcaag ccgactctcg cgctctctcc cgagctcttc ttgtcttccg
     4801 gatatacggg ttctggtgcc tcgac
//
LOCUS       DASTKU010000694         5807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1249033, whole genome shotgun sequence.
ACCESSION   DASTKU010000694 DASTKU010000000
VERSION     DASTKU010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249033"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFQ61_24185"
     CDS             complement(<1..451)
                     /locus_tag="VFQ61_24185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9957627.1"
                     /translation="MLAQAGDIEVVGEAENPFVARDRIVELSPDVLLLDVEMPHMDGV
                     TFLRRLMHYRPMPVVICSSRTERAGKVALEAMQAGAVEVICKPNENYPPDQMAVDLIA
                     AIRTAAISVRVTTKASDARAMASAAPAVVRNSLPSPRNAFDLIAMGGS"
     gene            complement(510..965)
                     /locus_tag="VFQ61_24190"
     CDS             complement(510..965)
                     /locus_tag="VFQ61_24190"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET9957628.1"
                     /translation="MMTPSPELLADTAAPILAECAFLCTTPARGKQDWATSVSIASLR
                     FSGPRSGRIEVCAAFDLLKSLAADMLGIDPSDSDASRHADAALAEITNVVTGRLVANL
                     FGTSSTWQLSLPEVSHGPVPPLGERAISQTLVDPDLRPIRVTLYLGKAA"
     gene            complement(962..1348)
                     /locus_tag="VFQ61_24195"
     CDS             complement(962..1348)
                     /locus_tag="VFQ61_24195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9957629.1"
                     /translation="MLRVLVVDDSPVMRRMLTKSLEMAELPIQTIYEAGDGQTALDVL
                     ANNWIDLVLCDINMPQMNGVELMEHISRSAAMGLVPVIMVTTETSEPRLDYLKKLGVR
                     ACLAKPFRPEALGETVRQILHLESEA"
     gene            complement(1367..1852)
                     /locus_tag="VFQ61_24200"
     CDS             complement(1367..1852)
                     /locus_tag="VFQ61_24200"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HET9957630.1"
                     /translation="MSVITVGVGDMQVSANPDAVLVTYALGSCIAVCVYDATKQAGGM
                     IHYMLPNSAVSPERAQREPAVFADTGVPMLFERLYALGCKKPNLIVKVVGGANIRDPD
                     CTFDIGQRNYLVLRKMFWKVNVPVKAEDVGGNATRTARLCLKTGTLTVRISDRGTEVE
                     L"
     gene            complement(1849..2664)
                     /locus_tag="VFQ61_24205"
     CDS             complement(1849..2664)
                     /locus_tag="VFQ61_24205"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET9957631.1"
                     /translation="MDSVTFGAFQKLAYQHAGITIGENKRALLHARLTKRMRKLGMTK
                     AEHYLAYLSQDRSGTELMGFLDAITTNFTSFFRENEHFEFLSRELKNRLQEGARMFRV
                     WCAAASTGEEPYSLGITLAEALEGSQADFRLLATDISMQALTEAHEGCYEAARLRPIA
                     PHLVRKYFEPAREQEDDQMLRVRDALRAKIVFKRLNLATPPFPMRGDIDFVFCRNVMI
                     YFDDATRQRLVAAIFEMLRPGGVLFIAHSETLTRVPSDLNLVQPSVYRKGCSI"
     gene            complement(2668..4758)
                     /locus_tag="VFQ61_24210"
     CDS             complement(2668..4758)
                     /locus_tag="VFQ61_24210"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9957632.1"
                     /translation="MAMKLKVKLLLAFASVALASGLVGVVGWHDAQRFRDALTETTES
                     LAPSLLHTGSARSSQARVQLFSSRAIVATLEGKTVEVPGLRRARDEAMRELESSLDSI
                     SKLKLPESTREAARLASSRLRDWQTHNNEIWRAIEAGDGKQAREIVEHVSAPSGEAAV
                     QAIDALTTATREVLDESSKRRDVITEDAFSELSLMTLIIMLASLGVGFTIATAITRPL
                     RDLEKSATRVAEGDFGHTIEHRSQDEIGALADSFRSLQQYVADISEAAKSLSEGRLET
                     KLTPRSEHDELSRGMMSATAVLREVLMEFERCILSARHGELSRRADTTRFPGVYGELL
                     GGMNNLLSAVAEPINEASRVLERMAARDLTSRARDDLPGDYRTMMTSVNRAAENLKDS
                     LLQVSSASEQVASASSQIAQSSQSVAQGASEQASALEETSSALVEISTTTRRNAEDAK
                     VANEIAQEARKASANGGAAMVQMTEAMSQIRAAAEATAAIIRDINDIAFQTNLLALNA
                     AVEAARAGEAGKGFAVVAEEVRNLALRSKEAAKKTELLIGQSMSLTQQGDQIRDHVSQ
                     TLEAIVGSVDRVGKIVDQISTASRGLAEGIEQSQRAISQMDQATQQAAANSEETSSAA
                     QELASQAQELSSLVARFRVEDSGGGGAKVSPIRPLRAASPQRKSGKQQRAEGGAALAR
                     NLIPIEDDPDFREF"
     gene            complement(4793..5302)
                     /locus_tag="VFQ61_24215"
     CDS             complement(4793..5302)
                     /locus_tag="VFQ61_24215"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9957633.1"
                     /translation="MTMVTQGGPGADTASLAGKYMTFRLATEDYGFEILKVRELIGLM
                     EITRVPRSPSYIRGVINLRGKVIPVVDLRVKFEMESVEPTSQTVIIVVQLGPQHGGLT
                     MGVMVDEVLEVRAIESKDVEPPPTFQSQTATDFILGVGKAENRVVFLLDIDRILSVKE
                     TTNVSEGLF"
     gene            complement(5299..>5807)
                     /locus_tag="VFQ61_24220"
     CDS             complement(5299..>5807)
                     /locus_tag="VFQ61_24220"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9957634.1"
                     /translation="NSTPGAGTSFKLILPLTLAIIEGMLVACGKERYIIPSLMIVESV
                     QPTSEMVRTPSRDTEVINVRGELMPLLRLARLFDIPESEQDPANARVVVVESAGSKLG
                     LMVDDVLTQQQVVIKPLSAGLGDTDFLAGAAILSDGRVGLILNVERLSSVFGARRRST
                     SESREFAA"
BASE COUNT          981 a         1825 c         1887 g         1114 t
ORIGIN      
        1 tggagccgcc catagcgatc agatcgaagg cgttgcgcgg gctcggcagc gaattgcgca
       61 cgacggccgg ggcggcgctc gccatggcgc gggcgtccga agctttggtc gtcacgcgga
      121 ccgaaatcgc ggcggtgcga atggccgcga tcagatcgac cgccatttga tcgggcggat
      181 agttctcgtt gggtttgcag atgacctcga cggcgccagc ttgcatggct tcgagcgcga
      241 ctttgccggc gcgctcggta cgcgaagaac agatcacgac cggcatgggt cggtagtgca
      301 tcagccgccg caggaaggtg acgccgtcca tgtgcggcat ttccacgtcg agcagcagca
      361 cgtcgggcga cagctccacg atgcggtcgc gggccacgaa cgggttttct gcttctccga
      421 ctacttcgat gtcgccggcc tgcgcgagca tgcgcgcgaa caggcggcgc gcggcactag
      481 agtcgtcgac caacaatacg cggatcacgt taggctgctt tcccgaggta caaggtcacg
      541 cggatggggc gaagatcggg atccacgaga gtttggctga tggcgcgctc cccgagcggg
      601 ggaacggggc cgtgggagac ttcgggcaac gagagctgcc aggtggagct cgtgccgaac
      661 aggttcgcga ccaggcgacc cgtgaccacg ttggtgatct cggccaaggc cgcgtcggcg
      721 tggcgagacg cgtcgctatc gctcggatcg atgccaagca tgtcggcggc cagagatttg
      781 aggaggtcga acgcggcgca cacttcgatg cgcccggagc gcgggccgct gaagcgcagg
      841 gacgcaatgc tgacgctggt cgcccaatct tgcttgccac gcgcgggcgt ggtgcaaagg
      901 aaagcacact cggcgaggat gggcgcggcg gtgtcggcca gcagctccgg gctgggggtc
      961 atcatgcttc ggactccagg tgcaaaattt ggcggacggt ttcgccgagg gcctccggtc
     1021 ggaagggctt ggccaggcac gcacgcacgc ccagtttttt caggtaatcc aagcgcggct
     1081 cgctggtctc ggtagtgacc atgatgaccg ggacgagccc catcgccgcg ctgcgggaga
     1141 tgtgctccat cagctccacg ccgttcattt gcggcatgtt gatgtcacac agcacgagat
     1201 cgatccaatt gttggccagt acgtccaagg cggtttggcc gtcccccgct tcgtagatgg
     1261 tctggatcgg aagctccgcc atttccagag acttcgtcag catgcgccgc atgaccggag
     1321 aatcatcgac tacgagtact ctcagcattt caggattcct tccgagtcaa agttcgactt
     1381 ccgtgccgcg atcgctgatg cgcaccgtga gtgtgccggt cttcaggcag aggcgcgccg
     1441 tgcgggtggc attgccgccc acgtcttcgg cctttacggg cacattgacc ttccaaaaca
     1501 tctttctcaa aactagatag ttgcgttgcc cgatatcgaa cgtgcagtcc gggtctcgga
     1561 tgttggcgcc cccgaccact ttgacgatca ggttgggctt cttgcagccg agcgcgtaca
     1621 gccgctcgaa caacatgggc acgccggtgt cggcgaagac cgccggctcg cgctgagcgc
     1681 gctcgggcga cacggccgag ttcggcaaca tgtagtgaat cattccgccc gcttgcttcg
     1741 ttgcatcgta gacgcatacg gcaatgcagg agccgagcgc gtacgtcacg agcaccgcgt
     1801 cggggttggc ggagacttgc atgtctccca cacctacggt gatgacgctc aaatcgagca
     1861 gcctttgcgg tagacggagg gctgaaccag gttcaaatcg gagggcactc gggtcaaggt
     1921 ctccgaatgc gcgatgaaca acaccccgcc gggtcgaagc atctcgaaga tcgcggcgac
     1981 gagccgctgc cgggtcgcgt cgtcgaagta gatcatgacg ttgcggcaaa acacgaaatc
     2041 gatgtcgccg cgcatgggga agggcggagt tgcgaggttc agtcgcttga acacgatctt
     2101 tgcgcgcagc gcgtcccgca cccgcaacat ctgatcgtcc tcttgctcgc gggcaggttc
     2161 gaagtacttt cgaaccaggt ggggcgcgat ggggcggagg cgagcggcct cgtagcagcc
     2221 ctcgtgggct tcggtcagcg cctgcatgga aatatccgtg gcgagaaggc ggaagtcggc
     2281 ttggctgcct tccaacgctt ccgccaacgt gatgcccagg ctgtagggct cctcgcccgt
     2341 cgacgcggcc gcacaccaca cgcgaaacat ccgagctccc tcctgcagcc gattcttgag
     2401 ttctcgactc aggaactcga agtgctcgtt ctcacggaag aagctcgtga agttcgtggt
     2461 gatggcgtcc aagaagccca tcagctcggt gccgctccga tcttggctga ggtaagccaa
     2521 gtagtgctcg gccttggtca tgccgagctt gcgcatacgc ttggtgagcc gggcgtgtag
     2581 cagggcgcgc ttgttctcgc cgatcgtgat gccggcgtgt tgataagcga gcttctggaa
     2641 cgccccgaac gtcaccgagt ccatggctca gaactcccga aagtccggat cgtcttcgat
     2701 cggtatcagg ttgcgagcca gcgcggctcc gccctccgcg cgttgctgct ttccgctctt
     2761 gcgctgtggg gacgcggcgc gcagcggtcg aatcggcgag accttggctc caccgccgcc
     2821 cgagtcctcc acgcggaagc gcgcgaccaa gctcgagagc tcctgggcct gagaggcgag
     2881 ctcctgggcg gcgctggagg tttcctccga gttggccgca gcttgctggg tggcttggtc
     2941 catctgcgag atggcccgtt gcgactgctc gatgccctcg gcgaggccgc ggctggcggt
     3001 cgaaatctga tcgacgatct tgcccactcg atccaccgag ccaacgatgg cctcgagcgt
     3061 ctggctgacg tgatcgcgga tttgatcgcc ttgctgggtc aaggacatgg actggccgat
     3121 caagagctcg gtcttcttgg cggcttcctt gctgcgcagc gcgaggttgc gcacctcttc
     3181 ggcgaccacg gcgaagccct tgcccgcttc gccggcccgc gccgcttcga cggcggcgtt
     3241 caaggcgagc aggttggtct ggaacgcaat gtcgttgatg tcccggatga tggcggccgt
     3301 ggcctccgcg gccgcgcgga tttgcgacat ggcctcggtc atctgcacca tggccgcgcc
     3361 gccgttggcc gaggcttttc gcgcttcctg agcgatctcg ttcgcgacct tggcgtcttc
     3421 ggcgttgcgg cgcgtggtgg tcgagatctc gaccagcgcg ctcgacgttt cctccagcgc
     3481 gctggcctgt tccgaggcac cttgggcaac cgattggctg ctctgcgcga tttgtgacga
     3541 ggcggaggcg acctgttcgg aggcggagga cacttgcagt aagctgtcct tcaagttttc
     3601 cgcagcccgg ttcaccgacg tcatcatcgt ccggtaatcg ccgggtagat cgtcgcgcgc
     3661 acggctcgtg aggtcgcgcg cggccatgcg ctcgagcacg cggctggcct cgttgatcgg
     3721 ttccgccacg gcgctgagca ggttattcat gccgcccaag agctctccgt agactcccgg
     3781 gaagcgggtc gtatcggccc ggcgcgagag ctctccgtgt cgcgcggaca ggatgcagcg
     3841 ctcgaactcc atcaacacct cgcgcagcac cgcggtggcg ctcatcatcc cgcgcgatag
     3901 ctcgtcgtgt tcggagcgcg gcgtcagctt ggtctccagc cggccctccg agagcgattt
     3961 agcggcctcc gaaatgtccg ccacgtattg ctggagcgag cggaaggagt cggcgagcgc
     4021 gccaatttcg tcttggctgc ggtgctcgat ggtgtgtccg aaatcacctt ccgcgacgcg
     4081 cgtcgcggat ttttcgagat cccggagcgg gcgcgtgatg gcggtcgcga tcgtgaagcc
     4141 cacgccaagg gaggccagca tgatgatcag ggtcatcagg ctaagctcgg aaaaggcgtc
     4201 ctcggtgatc acgtcgcgcc gcttgctcga ttcgtccaag acctcgcggg tggcggtggt
     4261 cagggcatcg atggcttgca ccgccgcctc gcctgagggc gcggagacgt gctccacgat
     4321 ctcgcgagct tgtttgccat cgcccgcctc gatcgcgcgc cagatttcgt tgttgtgcgt
     4381 ctgccaatca cgcagcctgg agctcgccaa gcgcgcggct tcgcgggtgc tttcgggcag
     4441 cttcagcttg ctgattgagt ccagtgagga ctcgagctcg cgcatggcct cgtcccgagc
     4501 gcgacgcagt cccggaactt cgaccgtctt tccctcgagg gtggccacga tcgcgcggct
     4561 cgagaacaac tgcacgcgag cctgactact gcgcgccgat ccggtgtgga gtagggacgg
     4621 cgcgagcgac tccgtcgttt cggtcagtgc atcccgaaat cgttgcgcgt cgtgccagcc
     4681 aacgacgcct accaagcccg aggcgagcgc gacactcgcg aacgccagga gcagtttgac
     4741 cttcaacttc atcgccattt acgttctcgc tcttgcgccc ccgcgtggaa gctcagaaaa
     4801 gaccctcact cacgttggtc gtctctttga cgctcaagat tcgatcgatg tcgagcagga
     4861 agacgacgcg gttctctgcc ttaccgacgc ccagaatgaa gtcggtagcg gtttgacttt
     4921 gaaaggtggg aggtggctcc acgtccttgg attcgatggc gcgcacctcg agcacctcgt
     4981 ccaccatcac ccccatcgtg agcccgccgt gttgcgggcc gagctgcacg acgatgatta
     5041 ccgtttggct ggtgggctct acggattcca tctcgaactt gacgcggaga tccaccaccg
     5101 ggatcacctt gccgcgcaag ttgatgacgc cgcgaatgta gctcgggctg cgcggcacgc
     5161 gtgtgatctc catcagacca atcagctcgc gaaccttgag gatttcgaag ccatagtcct
     5221 cggtcgccag ccggaacgtc atgtatttgc cggcgaggct cgccgtgtcc gcacccggac
     5281 cgccctgggt caccatcgtc atgctgcaaa ctcccgggat tcgctggtgc tgcgccgacg
     5341 agccccgaat acactactca agcgttctac gttgaggatc aaaccgactc gaccgtcgga
     5401 caggatcgcc gcgccggcga ggaagtcggt gtcgcccagc cccgcgctga gcggcttgat
     5461 gacgacctgc tgctgggtga gcacgtcgtc caccatcagc ccgagcttgc tgcccgcgct
     5521 ctcgaccacc acgactcgcg cgttggccgg atcctgctcg gactctggga tgtcgaacaa
     5581 gcgagccaac ctcaagagcg gcatcagctc gccacgcacg ttgatgacct cggtgtcccg
     5641 actgggagtg cgcaccatct cgctcgtggg ctgcaccgac tccacgatca tcaggctcgg
     5701 aatgatgtat cgctccttgc cacacgcgac caacatgcct tcgatgatgg ccagggtgag
     5761 cggcaggatc agcttgaagc tggtgcccgc ccccggcgta gagttga
//
LOCUS       DASTKU010000695        30943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1695414, whole genome shotgun sequence.
ACCESSION   DASTKU010000695 DASTKU010000000
VERSION     DASTKU010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 30943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30943
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695414"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFQ61_24225"
     CDS             complement(<1..352)
                     /locus_tag="VFQ61_24225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957635.1"
                     /translation="MKARAPGKLVLSGAYAVLEGAPAIVAAADRYVVADSSRPAGFIT
                     PEVRAALPSGPWPAFDASPLREGDRKLGLGSSAAILVASLAALELERDPELAAAELAE
                     RVFRPALVAHREAQG"
     gene            complement(349..1353)
                     /gene="mvaD"
                     /locus_tag="VFQ61_24230"
     CDS             complement(349..1353)
                     /gene="mvaD"
                     /locus_tag="VFQ61_24230"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET9957636.1"
                     /translation="MTHVASALACSNIALVKYWGKADVERNLTAVPSLSLTLAALTTR
                     TRVEFDPNSNEDTLEINGSVLDGTPVTRAVRLLDEVRSLARIQTRARVTSFNDFPTAS
                     GLASSASGFAALALAACAAAGVELPPSATSSMARRASASAARSVFGGFVELLAGAEAA
                     QPLVDPEYLDVAMIIAATTLGPKAVGSTSGMLNTQRTSPYYPAWVASAEPLFREAKAA
                     LLAKDLERLGEAMEASTLAMHACMLAARPSLCYLRDSTLSLMEEVRRLRSSGVAAFFT
                     MDAGPHVKVLVSRADSARVRDALAAVPGVERVIVSGPGAAAHVLAPSVDGPARAGVAP
                     "
     gene            complement(1350..2276)
                     /gene="mvk"
                     /locus_tag="VFQ61_24235"
     CDS             complement(1350..2276)
                     /gene="mvk"
                     /locus_tag="VFQ61_24235"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET9957637.1"
                     /translation="MAFANGKLILLGEHAVVYGSPAIACGIQRGVFARADPAQVGELV
                     IGERRISADPTSGNDLSRALQALLGELALGPHCITLRIELPTGAGLGASAAMGVACAR
                     AVLEAHGLPCESRRVLAAAGAWERVFHGNPSGIDAAAAERGGCLWFTKAEGIQPLALA
                     CDLPAVVAIAGPPASTREMVEQVARLAAAKPELVQRSMSSITALVRNGRTALEHGDFA
                     ALGQLMNLNQMLLAGLFVSTDGIERACGVARDAGAWGAKLTGSGGGGAIVAIAEDPER
                     VVSALRSEGFESFVTQVQAVPSSGLDPMRVES"
     gene            complement(2356..3447)
                     /locus_tag="VFQ61_24240"
     CDS             complement(2356..3447)
                     /locus_tag="VFQ61_24240"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET9957638.1"
                     /translation="MPQSKNRPNQPSTESTRRVRPEPFRELLGQVQKEIDRRLDEQLA
                     RELERDSNVAVEVKAMLSALRALCSRGGKRLRPALVMVGYRASKARVRGELVYRVGVA
                     LELIQAYFLIHDDWIDGDLVRRGGPSVHAALSRRFRNEHIGASSAILAGDYAAALATG
                     ILASLDLPAPAAGRVLRAFSEMQLHAVAGQQMDLMAKSKDIELAYVLKTGSYTVHGPL
                     LLGALLAGAPASVERCLGRLARPLGVSFQLRDDLLGAFGDPKLTGKPLGNDLTAGKRT
                     LLVMEALKRARPAGRRVLKSVLGNAQAELALVKQALDVIESSGARHAIEQRVTELAAE
                     ARAALGPELTRDGRQLLEGAIQALSERAT"
     gene            3679..4716
                     /locus_tag="VFQ61_24245"
     CDS             3679..4716
                     /locus_tag="VFQ61_24245"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9957639.1"
                     /translation="MPATYVWPMSSLAPFTKAAYQAQQAAVHWSLVALHLGLRPFVTR
                     KNEPDTRALQALRERLSELVALDLANVERGAYPSELLFQLPVREYLAGLPKLAGEVTR
                     MIARARRGRTVELPRELDLSHYPEYFRRNFHWQTDGYFSSRSAELYDLGVEFLFLGTA
                     DIMRRQIIPPLAPLLAGPEPRRVLDVACGTGRSLYQLSRAYPRHRYFGVDLSPFYIER
                     ARELLRGRSVSLLVENAESLTLRDASFDAVTSTFLLHELPKSARHRVLAEMRRVLSPG
                     GLLVLEDAAQLGDAPELEVFLKNFAHTMNEPYFKTHIESDLATELAGHGFRIVEQQPA
                     FLAKVVVARAE"
     gene            4865..5929
                     /gene="fni"
                     /locus_tag="VFQ61_24250"
     CDS             4865..5929
                     /gene="fni"
                     /locus_tag="VFQ61_24250"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET9957640.1"
                     /translation="MTDIAQRKADHLDLAEKGDVAFKQRTTLLECVELIHDALPELDY
                     DELDASLQLFGKRLRAPILIAAMTGGHPRAERINKELAEVAESLGFAFGLGSQRAMHR
                     DPKARASYDVRSVAPNALLFGNIGVVQARGMKSAELRELVESVGADALCVHLNPAMEL
                     VQVDGDRDFRGGLETLQRLSSELPVPVIAKETGCGLSARAGARLKKAGIRHVDVSGAG
                     GTSWVAVETHRARDERKALGETFWDWGIPTAASVGSLRPFAFDTLIATGGVQNGLDAA
                     RAIALGAHLVGIARPTLQALDRGGPSEAEAFLRRVESELKTAMLLTGSPNLDALRAAP
                     RILRSELRDWLHGAPDTSGG"
     gene            complement(5961..6290)
                     /locus_tag="VFQ61_24255"
     CDS             complement(5961..6290)
                     /locus_tag="VFQ61_24255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957641.1"
                     /translation="MDATVSAIEPAEIDRLIDASDFAGARSALASYPEGDERVIVLRI
                     KLGLREERLPPGAAMQQLIQIMRQNEHFPGAKELYQEASSLAWQARESNVAHSHPPPP
                     ATPRRPR"
     gene            6570..7421
                     /locus_tag="VFQ61_24260"
     CDS             6570..7421
                     /locus_tag="VFQ61_24260"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9957642.1"
                     /translation="MSEENRRRSEHSDAPAPDLRIERDQFLRKFSRGARLTEDFLRAY
                     EELELRCAALESENGVLRTQVEAGDAIRELLNKIQRLEQEKDELLSRFRRVEAQSSTV
                     SARVQEVEHEFANLANLFVASNQIHSSLSPRGVTRRIKEVLAQFVGAERYSMYLVNPE
                     GTELVPIAFEGVPASELAAVRVSGTFLGQIFSSRTAFVDEDHDASQGTFDRPAAVIPL
                     SVDERVVGVIAIFATLSQKPRFDTVDFELFKLLGEHAAAALVSASLFVQADRKLPGLE
                     AFLDLSV"
     gene            7443..7820
                     /locus_tag="VFQ61_24265"
     CDS             7443..7820
                     /locus_tag="VFQ61_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9957643.1"
                     /translation="MAEYSCLIVEDSPMMRQLLVFALARVKNLRVTEADDGVDGLRKL
                     ASARYDIIVTDINMPIMDGLKLVKRVRSDPVHKDTPIVIITTEGSQEDRQRALQLGAN
                     AYITKPIQAPQVIATVKELLRIE"
     gene            7841..7972
                     /locus_tag="VFQ61_24270"
     CDS             7841..7972
                     /locus_tag="VFQ61_24270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957644.1"
                     /translation="MAQSRGMGRLRAEIIREHIGVREHIGIREHIGVREEVTQCGLG"
     gene            7957..8580
                     /locus_tag="VFQ61_24275"
     CDS             7957..8580
                     /locus_tag="VFQ61_24275"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET9957645.1"
                     /translation="MRPWLKICGITCDEDADLVVDAGADAIGLNLVPRSKRYIDRKLA
                     RRLADRVRGKLEIVCVVADESLGSLRELQTELAPDWLQLHGEEPAAWLNELPRSFKAV
                     GLAEPSDALAADAYPGSRLLVDAKSGGSSGGTGLSFDWAWAAELCRKRDVILAGGLNP
                     ENVAQAYAALHPFGLDVASGVDSPGNPRRKDPLRVAQFVARAHAAAQ"
     gene            8537..9124
                     /locus_tag="VFQ61_24280"
     CDS             8537..9124
                     /locus_tag="VFQ61_24280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957646.1"
                     /translation="MSRSSWRVLMRRRNKLTPILSGAPGWVHARSASMLLGLLASLAV
                     PFASSCGGPNLRGALAVDRGTEPRDGELSSYSVRSCSDATGAPASGAGPRVSHQRSQK
                     RDILVEFRPRYSSVVIVNTYAEANEQVFAYVSDDAQRAAILHEYRIPQPDNASGGRLL
                     LFDRYTLAKDGDTFRRTMGSPIATCTLVPIATDLR"
     gene            complement(9157..9810)
                     /locus_tag="VFQ61_24285"
     CDS             complement(9157..9810)
                     /locus_tag="VFQ61_24285"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9957647.1"
                     /translation="MRFLCVSDIHGHARELDAILEEADGYGWDQLVACGDLVFPGPEP
                     LKVWKTLVRHRALCVQGVSDRALSQIDPSKLLATTEHERERIERFRQIQGELGELIIT
                     RLGKLPTIARLPLESGHTLMVVHGSPLDPTECFTMDMTEDELIALLGDEPGDVIVCGG
                     SHAQFERRVADVQIISVGSVGQAPGGGYAQATIVATGATGIQVTPLSVNLPELPKYS"
     gene            9957..12851
                     /locus_tag="VFQ61_24290"
     CDS             9957..12851
                     /locus_tag="VFQ61_24290"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HET9957648.1"
                     /translation="METTRALLALANRIDRDRAAILRAEFGPSPERVPAVLLGAAFPA
                     LTPSSEFKLQAVEQLARQGLRVERRRRDLVRDVLSAVPGDAEPEAFGRALRRFVWAER
                     ARIALRELLPLELGGASIEVTARELSVLSEAALELALREAARTMRSRYGAPIRSDGGE
                     SSLCAFGMGKLSGHELNSGSDVDLLFVYDTDDGQSALTLHEHWSRVVRRAVSTIDSLT
                     EDGQIWRVDLRLRPEGSSGPITNSVAATERYYETWGRLWERAALLRSRPIAGNLELGA
                     LIEREIFTPFVYRHAVDPGLAGPLCAMVEQGRNELSPDPTRDLKLGPGGIREAEFFVQ
                     ILQLVWGGRDPNLRVPGTLAALARLASRGLVTDRESRAISSAYVFLRLLEHRVQWASG
                     LQTHLMPDQPADLDKLGRTLGYPDAAPMLAELSRARAAISEAFAGVLPGRVSRRQQRS
                     PFFSLLSALNDPVRLAREAERLFGSADLGEHLAALSRRPDGLLGELTQDRAPGLAGRL
                     LEALSTSPDPEQAARYLRSFFARLVTPMAYVTALGEDPRMLARFVTALSSSQFVGDAC
                     ISRPELADIVLFGEGKVSDPKAALELELESQPIDDPEEPDEGNQAFVTALCMARRRFM
                     VEVAVADLAGTLSARSTTRLLSDLADEVLRRAAERVLGPGAPGLAVIALGKLGGRDIG
                     YGSDLDVLFVYDPKRQSSREEAHLFYTRAAQRILRLISEPSVAGPGYVLDTRLRPSGA
                     HGLLVTSLPAFARYHGIELEDPEETATATSSGAAWERQALLRARAAAGDAALGERVVK
                     IAEQAAYERGAPPVEEMHRLRQRMELELGRERAWRMDLKSGRGGLLDVEFCVQWLQMH
                     HGEDLGVRTTDTSEALEALHLFGYLSRPHFDALRDGYSFLRRLEQRIHILSGIASEVI
                     DLTTRGLPQLARRMGFRDGPGPSAKDQLLARYASVTQSVRAAYLEALELS"
     gene            complement(12953..13954)
                     /gene="hemB"
                     /locus_tag="VFQ61_24295"
     CDS             complement(12953..13954)
                     /gene="hemB"
                     /locus_tag="VFQ61_24295"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET9957649.1"
                     /translation="MSETTQLVHRPRRNRRTEAIRSLVRETHLTAGHLVMPLFVQEGT
                     GQRTEITSMPGQARLSIDLLLETAREAHALGVAGVALFPALPEGVKDARGTESLNRAG
                     LLPRAVRALKQALPGLLVITDVALDPYSSDGHDGVLIDGRIDNDETLPLLAGMAVAQA
                     EAGADWVAPSDMMDGRVGVIRAALDEAGYTDVGICSYCVKYASGFYGPFRNALDSAPR
                     SGDKKTYQMDPGNVRECLREIELDEAEGADCLMVKPGLPYADVIRYVREHSTLPVAAY
                     HVSGEYAMLKFAAERGVLDYERCLLESLVCLRRAGADIIFTYGAIEAARALRQTIKT"
     gene            14147..14695
                     /locus_tag="VFQ61_24300"
     CDS             14147..14695
                     /locus_tag="VFQ61_24300"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET9957650.1"
                     /translation="MSSKNSSEKAYLVITAVGPDRPGIVSDLSRVIHAAGANLEDSRM
                     AVLGGEFALLLLVSGSEAELSRVLADAQSQESTLGLRLMSKPTSGPRAARNVLPYTIR
                     VTGLDRPGIVQSVTGVLAHHRVNVRSLESRVTYAAESGTPLFTFEAELEVPSAVVLAA
                     LRKELTTRADEDNLDLSLDARV"
     gene            complement(14673..15365)
                     /locus_tag="VFQ61_24305"
     CDS             complement(14673..15365)
                     /locus_tag="VFQ61_24305"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET9957651.1"
                     /translation="MKTAGVKTAGGKTAKDLLRFRCTGCGNCCKEPLLPLTDTDLRSI
                     LDHTGDAVADVVRFVDRNGIDMDDEPEAFVILPQGKRVMVLRHQRGGCRYLDAQDRCG
                     IYDARPLGCRIFPFDPRFERSGKLRRLKLIQATDCLYELDGANDPSALYELHQRYVEA
                     TRVYHEKVAEWNRLQRKLKRADKPLKRVRKFFEFLGVPVMPGRTSSPPSDYAAAPQTA
                     VDAPTLKPARPG"
     gene            15672..16781
                     /locus_tag="VFQ61_24310"
     CDS             15672..16781
                     /locus_tag="VFQ61_24310"
                     /inference="COORDINATES: protein motif:HMM:NF003711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HET9957652.1"
                     /translation="MSLKIDQDHSRFRSIVRGRIRQNLRRYISQGEMLGRKGKDLVSI
                     PIPHIDIPRFVFGDRQKGGVGQGDGQVGDGVGGDEAEQEGSGQAGKDAGDHVLEVDVT
                     LDELADILGEELELPAIENKGKSRIIAAKDRYTGIRRVGPESLRHFKRTYREALKRAI
                     ASGVYDPGMPLVVPQPEDKRFRSWKEDLEPVANAVILYMMDVSGSMGDEQKEIVRIES
                     FWIDTWLRRQYKGLESRYIIHDAVAREVDRETFFKTRESGGTMISSAYRLAVQIIDQH
                     YPSEEWNIYPFHFSDGDNWSMEDTLTCVELLKKEMLPKVNMFAYGQVESPYGSGQFIK
                     DMREHFGADERVVTSEIRDRDAIVSSIKDFLGKGK"
     gene            16781..18346
                     /locus_tag="VFQ61_24315"
     CDS             16781..18346
                     /locus_tag="VFQ61_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HET9957653.1"
                     /translation="MSQFATNTSLPRYLRVEQERIEKIAQKLGLDFFPVVFEMLSYDQ
                     MNEIAAYGGFPNRYPHWRFGMEYERLSKSYEYGLSKIYEMVINNNPSFAYLLEGNSLT
                     DQKLVMCHVFGHVDFFKNNFSFRATDLDAGDPVTDPMAKGGANYDPNRKWIDKMANHG
                     AAVRRIVDRYGIQRVEEFIDNCLSLENLTDPWSRFVVRRREREEEEEPKEIEVPRLRA
                     KDYMEEFINPSDYIEKERKRLTQERDRAKGRHPAHPEQDVLLFLIENAPLERWERAIL
                     SIVREEAYYFVPQMQTKIMNEGWASYWHSRMMTEQVCDWSEIVDYAENNAGVMATANG
                     RLNPYKLGVELFRSIEDRWNRGQFGPEWEQCDDLDVKRNWDLRLGLGRQKIFEVRALY
                     TDVTFIDEFLTEEFAIEHKLFTFNWSNRNERFEIESREFRQVKEKLLSQLTNFGNPVI
                     SVLDANFDNRGELLLRHDHRGIDLRSDYAKETLTALHRVWRRPVCLATQLDGKRALLR
                     YDGKEHSSRPDAP"
     gene            18475..19677
                     /locus_tag="VFQ61_24320"
     CDS             18475..19677
                     /locus_tag="VFQ61_24320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9957654.1"
                     /translation="MTAISWACAGAPAGQPQAPVAVRSNINTRDSSGSLSPEAEDAAA
                     PGGEAARENPPSSGPPDGSPHGAARTPGPAGSANGPGTRAVPGGLSRGGDSELERALS
                     AGDAAYFDGELPRARAEYERALKLAPQDLAPQLGLLRVELAGLDVPLDYAAAPNDARL
                     LGLLKRLDMLKARDPGYGPTELERGRVLLILGRAAPALQALERGVATLPKDPEAHSAL
                     GVALLATGRAAEALPRFRKAYELEPANAERARALGTAELLTGNVDRAIAAYSRAVALA
                     PTDARAHSDLGSAYLAQNAPVQAVPELQRAVDLDPTRATFRSNLAYAALLVGDNPAAA
                     EQARHAIRLDPKLASAHLNLGTALARQGRYDDAERAFRRALELDPSDPRAKTNLEELQ
                     ALRRQKPQ"
     gene            19940..20818
                     /gene="rfbA"
                     /locus_tag="VFQ61_24325"
     CDS             19940..20818
                     /gene="rfbA"
                     /locus_tag="VFQ61_24325"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET9957655.1"
                     /translation="MKGIVLAGGSGTRLDPVTRAVSKQLLPIYDKPLVYYPISVLMMS
                     GIREILIISTPHDLPLFRRLLGTGEELGLTFEYAEQAAPNGLAEAFIIGKQFIGSDSV
                     CLVLGDNIFYGAGLVKTLVDARAQLDGAVLFGYRVEDPERYGVAEFAPDGTLLGIEEK
                     PRAPKSQVAVTGLYFFDNQVVEIAAGLKPSARGELEITDVNNAYIQQRRAKIQVLGRG
                     FAWLDTGTQQSMLDAAHFVATLERRQGTYIACLEEIAYRSGYIDASRLRALAARCGKS
                     EYGRYLYRLLDEKATP"
     gene            20835..21380
                     /gene="rfbC"
                     /locus_tag="VFQ61_24330"
     CDS             20835..21380
                     /gene="rfbC"
                     /locus_tag="VFQ61_24330"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET9957656.1"
                     /translation="MQASDLRLSGLKLIRPQVFRDERGFFVETYHAQRYRDANIGVEF
                     VQDNYSRSARGTLRGLHYQSSPGQAKLVRVTAGRIYDVVVDIRPHSPTFGQWEGLHLD
                     SEEHLQLFIPVGFAHGFCAVSEVADVQYKVSNFYSGATECGLRYDDPDIGVVWPISDP
                     VLSERDRNAESFREFRARALK"
     gene            21377..22282
                     /gene="rfbD"
                     /locus_tag="VFQ61_24335"
     CDS             21377..22282
                     /gene="rfbD"
                     /locus_tag="VFQ61_24335"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HET9957657.1"
                     /translation="MKVWLCGARGMLGRAIEARLQSLAVACVPTDSEFDVTQGEPVIA
                     FARQHAFTHIVNCAAYTRVDAAESEPGTAHAVNALGASHLASAAAACAAHLLHFSTDY
                     VFDGAAHEPYREEAACAPRSAYGRTKLEGERLVLGTAPASMREARAVHVVRTSWLFGP
                     GGKNFVTTMLQLMAERETLRVVADQVGCPTYTYDLADASLALAAVGPSARAAAPSGIY
                     HFSNAEPVSWCGFAQAIHERALGLGFPIRAQAIQPIRTEEYPLPATRPAYSVLDTSKI
                     SGQLGRAPRPWRAALDEYLTTLRSS"
     gene            22282..23340
                     /gene="rfbB"
                     /locus_tag="VFQ61_24340"
     CDS             22282..23340
                     /gene="rfbB"
                     /locus_tag="VFQ61_24340"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET9957658.1"
                     /translation="MSAQRPTHNLLVTGGSGFIGSAFIRWLFAQPSYSGKIVNLDLLT
                     YAANPDNVSQVPADRYTFVHGDILDGKLVEETCRAHGVDTVVHFAAESHVDRSIHGPG
                     VFMHTNILGTYRLLEVVRALPGVHFHHVSTDEVYGSLGPQGYFTETTPYQPNSPYSAS
                     KAASDHLVRAYAHTYGVSVTISNCSNNYGPYQFPEKLIPLMIMNLLEGKPLPIYGDGS
                     NVRDWLYVDDHAEAIWLILQQAKSGETFNVGGNAEWPNLQLVHKLMDVVAELTGRSRE
                     ALQKQITFVADRPGHDRRYAIDCSKLQSQLGWTQRENIDSGLRKTVRWYLDNPLWLER
                     VRSGAYREWIDRNYGKRQ"
     gene            23508..24500
                     /locus_tag="VFQ61_24345"
     CDS             23508..24500
                     /locus_tag="VFQ61_24345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9957659.1"
                     /translation="MNSPSIAAIIVNWNSRDDVVDAVRSLLTQADSDVDIVVVDNGSK
                     DGSVEALQQFAPQVRVIETGANLGFAEGCNRGIEASNSDWVFILNNDAIVAPGGMARL
                     RAAAAAAPEDLGMLQPCIVFRTNPDQVNSSGVIVFSNGAARDRDFAAPVSATQQGSEV
                     FCCTAGAALYRRSMLQRVKRPTGYFDRTFFMYFEDVDLGWRCRLAGYRAVYFPEVVVL
                     HRFQASSSRRGRHFARSHSQANRVRSLIKNASPRMLARSIPRTVSDLTQLTMHDPRAL
                     GRLLRTLPELLQERQAIASLRQVDPARIEKDWMANKHFPAPKPDLPAPPKASSG"
     gene            24584..26200
                     /locus_tag="VFQ61_24350"
     CDS             24584..26200
                     /locus_tag="VFQ61_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET9957660.1"
                     /translation="MIRFDSINKGHGRQLLFLDASASVNRGEKVGLVGPNGSGKTTLF
                     RLIIGEEQPDNGNVVLDRGVTIGYFSQYIGDMRGKSVLSETMAGAGEVSRVAEELHQL
                     EHAMTHAAGDELEKAVERFGEVQARFDELDGYGLEAKAREILTGLGFRPEMIDGDVGR
                     LSGGWKMRVGLARILLMKPDLLLLDEPTNHLDLESILWLEAFLGAFEGALVMTSHDRE
                     FMNRLCSKILEIDGGELKTYSGNYDFYVAQRELDAAQKEAAFARQQAMLAKEQAFIDK
                     FKARASHAAQVQSRVKKLEKIERVEPPRRRKVVEFDFPSPPRSGEDVVKLEGVRKAYG
                     EHVIYSEFDFLIRRRERWCVMGVNGAGKSTLLKLVAGESNADGGRVSLGPSVVLGYFA
                     QHSMEILDPEKTVLEILTQAFPQSSLGSIKTLLGAFGFSGDDVDKRCRVLSGGEKARV
                     VLARMLFNPPNLLVLDEPTNHLDVDTKEMLVKALADFEGTLLFVSHDRRFLSALSNRV
                     LELGTGKPHAYGGGYTEYVRTTGHEAPGVS"
     gene            complement(26227..27507)
                     /locus_tag="VFQ61_24355"
     CDS             complement(26227..27507)
                     /locus_tag="VFQ61_24355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957661.1"
                     /translation="MSRARHTVGTILDRRYLLKREVARGAAGVVYEAQHLFTKRPVAV
                     KLLSETYAHVAETRNRLLREAIALATARHPFVAQVLDAGELPEFGPYLVMELLEGRTL
                     QGILAVRLRIGVHETVQVGRQLCEALAVAHGRGVLHRDIKPSNIFVARDETGREVVKL
                     IDFGIARTTSDSNKLTQQGAVLGTPEYMAPEQMMGHGQMDERVDIYAVGVTLYECLTG
                     NVPFDGSFGEVLLKSSTQEIPPIRAKSPTVPAELAATIEKALARDPNDRYPSMSAFVE
                     SLTQFPVPDTGATLLGLRALRPVARPAPVAPKLPAAAPPELPGVARRRFPRAPYVTPT
                     RILRPTGKGIDGRSEDISVAGLLVVTAAPCDADELVKVRFALPGSGKLVELAARTKWV
                     RNARGTEAVGLEFTAVPPDVHTAIEGYVTSMVGL"
     gene            complement(27771..29258)
                     /locus_tag="VFQ61_24360"
     CDS             complement(27771..29258)
                     /locus_tag="VFQ61_24360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957662.1"
                     /translation="MIVSRAGLVPCCLALLQVVGCGGAARNQRIGVDCVGTQSGRAEC
                     QEESASAASGSSVAGASSDQRGQPGRSARAGSRPTLPESKSTPGKPAVADAGEKAGDP
                     AGAAEPPAPPSYEPGRVRQALDATASAPDERTRLGLRLAIVDQGPNAPWLVAIANRGT
                     EPVRVNPDLRTLSLEITPKAPEPPQNAKYHRPVKPPKPVTCTLPRDLFPSVEDPNLRT
                     QLEPGEAVVDGFDPRLYCLSWTGTSPLKPGARVVARLGWPEKTKTVWKAGKSEKVLVA
                     QAPPFVAERLSGSTERDAGPASAPNDQSSSAAPGTAESSLLKQLVSASIELGPDYAEA
                     PPEADSEPLALQLTRGSDARTERDATLAVSIVNRSKSTQRVFFRRELLSFEISGPRGL
                     VTCEPGPDDRAPDRRAFQSLRPGGRISATSRLVELCPAGAMRSPGLYLVHARFEAREE
                     DAGEGSQPAYHGRLLSQKPVSIRVRRGWGVLPPQHEPMKVRLGTP"
     gene            29393..30238
                     /locus_tag="VFQ61_24365"
     CDS             29393..30238
                     /locus_tag="VFQ61_24365"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9957663.1"
                     /translation="MKQKRAPSPTRKQTVQTADPPELYTFAISHFSEKVRWALDIERV
                     PYREQRLIPGVHGFTTRGFAAKSSVPVLRQGNRVIQGSREILDALPVLFSATTLQPAT
                     LSPEQRAEIVEWEALFDYAFGLGVQRLAFDELLGLPREAVDFIAQDAPGWAKLLYRVG
                     YPFVRRSVRDLYEIKPRTVDRARRAFADAIARTDKRLENCPFLVAEQLTRADITLAAL
                     LAPLCRPPQHLARWPKTVPGLQEFVTPFESGVTWKWVLRMYSERRHGSGSAIEQQADS
                     ARRRE"
     gene            30353..>30943
                     /locus_tag="VFQ61_24370"
     CDS             30353..>30943
                     /locus_tag="VFQ61_24370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957664.1"
                     /translation="MSLERIRALVERFRRVIADGGASGPAVNGWQFDTAIGLELLESM
                     LLARHLDVAAHELRASGHGHYTICSSGHEANAVLGRLTTPDDSALLHYRSAALCLERA
                     RQVPEVDAVRDIALSLVAARDEPASGGRHKVFGNRRLGIIPQTSTIASHLPRAVGLAF
                     ALERKARLGLLPAIGSLAEPGSHTRRSPIVICSFGDA"
BASE COUNT         5654 a         9932 c         9955 g         5402 t
ORIGIN      
        1 cgccctgtgc ctcgcgatgc gcgacgagtg cgggacgaaa gacgcgctct gccaactcgg
       61 ccgctgcaag ctccgggtca cgttcgagct cgagcgcagc caagctcgcc acgagaatcg
      121 cggcgctgga ccctagtccg agcttgcgat ccccctctcg caaggggctc gcatcgaagg
      181 cgggccaagg acctgacgga agcgcggctc gaacttcggg ggtgatgaac cctgctggac
      241 gagagctatc ggcgactacg tagcggtccg cagccgccac aatcgcgggt gcgccctcca
      301 gcaccgcata cgcgcccgag agcaccagct tgccgggcgc tcgagccttc atggtgcaac
      361 tcccgctcga gcgggcccgt cgactgatgg cgcgagcacg tgcgcagccg ccccggggcc
      421 actcacgatc acgcgctcga ctccgggcac ggccgcgaga gcgtctcgaa cacgcgcact
      481 gtcggcgcgt gacaccaaca ccttcacgtg aggcccggcg tccatcgtga agaacgcggc
      541 gacgcccgag gaacgaagcc gacgcacctc ttccattagg ctcaaagtac tgtcccgcag
      601 gtaacacaag ctgggacgcg ccgcgagcat gcaagcatgc atcgccaagg tgctcgcttc
      661 catcgcttcg ccgagcctct ccaggtcttt tgccaacagg gcggccttcg cctcgcgaaa
      721 caagggctcg gcggacgcga cccacgccgg atagtatggg ctcgtgcgtt gcgtgttcag
      781 catgccggac gtcgatccga ccgccttggg accgagcgtc gtcgcggcaa tgatcatggc
      841 cacgtccaga tactcgggat caaccagggg ctgcgcggcc tccgcccccg ccaacagctc
      901 gacgaagccg ccgaacacgg aacgcgctgc agaagcggag gcgcggcgag ccatgctgct
      961 agtggctgaa ggcggcagct ccacgcccgc ggcggcgcag gcagccagcg cgagcgcggc
     1021 gaagccagac gcactcgaag ccaaacccga agcggtcgga aaatcgttga agctcgtgac
     1081 ccgagcgcga gtctgaattc gggccaggct ccgcacttcg tcgagtagac gaaccgcgcg
     1141 cgtcacgggg gtgccgtcga ggacgctgcc gttgatctcc agagtgtcct cgttcgagtt
     1201 cggatcgaac tccacgcgcg tgcgcgtggt gagggctgcg agcgtgaggg agaggctcgg
     1261 gaccgcggtc aaattccgtt cgacgtcggc cttgccccag tacttgacga gggcgatgtt
     1321 ggagcaagca agcgcgctag caacgtgggt catgattcga ctcgcatggg gtcgaggccg
     1381 gaactcggca cggcctgcac ctgcgtaaca aagctctcga aaccctcgct tcgaagcgcg
     1441 ctcaccacgc gctcggggtc ctctgcgatc gccacgatcg cccctccgcc tcccgatccc
     1501 gtcagcttgg ctccccaagc accagcatcg cgggccacgc cacacgctcg ttcgattccg
     1561 tccgtcgaca cgaagagccc cgcgagcagc atctgattca agttcatgag ctgtccgagc
     1621 gccgcgaaat ccccgtgctc gagcgcagtt cgaccgttgc gcaccaaagc ggtgatgctc
     1681 gacatactac gttgcaccag ctctggcttg gccgcggcaa gtcgtgccac ttgctccacc
     1741 atctcgcggg tgctcgcggg cggccccgca attgcaacca ccgccggtaa atcgcaggcc
     1801 aaggcgagcg gctgaatacc ctcggctttg gtgaaccaga ggcatccgcc gcgctccgcc
     1861 gcagccgcgt cgatcccgga tggattgccg tggaacacgc gctcccacgc gcctgccgcg
     1921 gcgagcaccc gtcgagactc acacggcagg ccatgggctt ccagaacggc ccgagcacag
     1981 gccacgccca tggccgcaga ggctccgagc cccgcgcccg tgggaagttc gatgcgcagc
     2041 gtgatgcaat gcggcccgag tgcgagctct cccagcaagg cctgtagcgc tcgcgagagg
     2101 tcattcccac tcgtaggatc ggcgctgatc cggcgctcgc cgatcacgag ctcgccgact
     2161 tgcgccgggt cggcgcgagc gaacacgccg cgctgaatgc cacatgcgat ggcgggcgag
     2221 ccgtaaacca cggcgtgctc acccaaaaga atcagctttc cgttagcaaa agccatggcc
     2281 tgttcgcaaa caacgcttct tacagtcttt cagtgctcgt aacacgagcg actgggtgtc
     2341 cacctgacta gcacgtcagg tcgccctttc gctgagtgcc tggatggcac cttcgagcag
     2401 ctgccgtccg tcccgggtca actccgggcc cagcgccgcg cgggcctcag cggccaactc
     2461 cgtcacacgc tgctcgattg cgtgccgcgc ccccgaggac tcaatcacgt ccaaagcttg
     2521 ctttaccagc gcgagctcgg cctgggcgtt gcccagcacg cttttcagca cgcggcgacc
     2581 ggccgggcgt gctcgtttca gcgcctccat gacgagcagg gtccgcttgc ccgcggtcaa
     2641 gtcgttgccc aagggcttgc ccgtgagctt ggggtctccg aacgcgccga gcaggtcgtc
     2701 tcgcaattgg aaacttaccc ccagtggccg cgccaagcgc cccaagcagc gctcgacgga
     2761 agctggcgcg cccgccagca acgcgccgag cagcaacggc ccgtgtacgg tgtagctgcc
     2821 ggtcttgagg acgtacgcga gctcgatgtc cttggacttg gccatcagat ccatttgctg
     2881 gcccgcgacc gcgtgcaact gcatttccga gaacgcgcgc agcacgcggc cggcggcggg
     2941 ggcgggtaag tcgagactcg ccaggattcc ggtcgccagg gccgcggcgt agtcacccgc
     3001 caggatcgcg ctggaagccc caatgtgctc gttccggaag cgtcgagaaa gcgcggcatg
     3061 aacgctgggg ccgccgcgtc gcaccagatc tccgtcgatc cagtcgtcgt ggatcaagaa
     3121 gtacgcttgg atgagctcca gcgccacacc gacgcggtac accagctcgc cgcgcacacg
     3181 cgccttgctg gcccgatatc caaccatcac gagcgccggt cgcagacgct tacctccgcg
     3241 agaacacaga gcgcgcaggg ccgagagcat agctttcacc tcgacggcga cattgctgtc
     3301 acgctcgagt tctcgagcca actgttcgtc caaacggcga tcaatctcct tctgaacttg
     3361 cccgagcagc tctctaaaag gttcaggacg cacgcgacgg gtggactccg tggagggctg
     3421 gttcggccgg ttcttcgact gtggcatcag ggcggcgcga gcctagagca aggagacctt
     3481 ggaaaccagc tgccgaacgc ggaaagtcca tcacacgccg agccgatcgc gctagctcgg
     3541 cgtacgcgcc gcacccgggc ccccgccgca cgctgtctag gctgccgagg tgtgcatctc
     3601 cgatccggag acggcgtgga cacgatagcg gcggtcccgc actgcgatag ttgaccaggc
     3661 gggagccttt acgagcccat gcccgcgact tatgtctggc ccatgtcttc gctcgcgcct
     3721 ttcaccaagg ccgcatatca ggcccagcag gcggccgttc actggtctct ggtagcgctg
     3781 cacctcggtt tgcgaccgtt cgtcacacgc aaaaacgaac ccgacaccag agctctgcag
     3841 gctttacggg aacgtctaag cgagctcgtg gccctggatc tcgcgaacgt ggagcgtggc
     3901 gcctatcctt cggagctctt gtttcagctg cctgtacgcg aatacttggc tgggctgccc
     3961 aaactagccg gtgaagtgac ccggatgatc gcgcgcgccc gccgcgggcg gaccgtcgag
     4021 ctgccgcgcg aactcgatct gtctcactat ccggagtact tccggcgcaa cttccactgg
     4081 cagacggatg gctatttcag ctcgcgctcc gccgagctgt acgacctggg agtggagttc
     4141 ctgttcttag gcacggcaga catcatgcgc cgccagatca tcccgccact cgcgcctctg
     4201 ctggccggtc cagagccgcg gcgggtactc gacgtggcat gtggcaccgg gcggagcctc
     4261 taccagctgt cgcgggctta ccctcgccat cgctacttcg gcgtggatct cagcccgttc
     4321 tacatcgagc gcgcccgcga gctgctgcgc ggacgcagcg tttcgctgtt ggtcgagaac
     4381 gctgaatctt tgacgttgcg cgacgcgagc ttcgatgcag taacgagcac cttcttgctg
     4441 cacgagctac cgaagtcggc acggcaccgc gtgcttgccg aaatgcgccg cgtgctcagc
     4501 ccgggcgggt tgctggtatt ggaggacgcg gcgcaactgg gggacgcgcc cgagctggaa
     4561 gtgttcttga agaacttcgc gcacacgatg aacgagccct acttcaagac ccacatcgaa
     4621 tccgacttgg ccaccgagct ggcgggccat ggcttccgca tcgtcgagca gcagcctgcg
     4681 ttcctggcga aggtggtcgt cgcacgcgcg gaatgagagc ccacgagccg aacggtgtgc
     4741 tcacgccgtt cgacggaggc gcttggacct ccgccggatg cgcgcgcgtg ctagcgtggc
     4801 gtttcggccc gcattcgaag tgaatcgccg cctgaagcgc gacgaccgga gcacgattga
     4861 tagcatgacc gacattgcgc aacgcaaagc cgaccacctg gacctggccg aaaaagggga
     4921 cgttgcgttc aagcagcgca cgactctgct cgagtgcgtc gagctcattc atgatgctct
     4981 tcccgagctc gattacgacg agctcgatgc gtcactccag ttgttcggga agcgcctccg
     5041 agcgccgatc ctgatcgcgg caatgaccgg tggccacccg cgcgcggagc gcatcaacaa
     5101 agaactcgcg gaagtggccg agtctctcgg ctttgccttc ggcctgggta gccagcgcgc
     5161 gatgcaccgc gacccgaagg cccgagccag ctacgacgta cgatcggtgg ccccgaacgc
     5221 gctgctgttc gggaatatcg gggtcgtgca agcacgtggg atgaagagcg cggagctgcg
     5281 cgagctcgta gagagcgtcg gcgcggacgc tctgtgcgtg cacttgaatc cagccatgga
     5341 gctcgtgcag gtggatggtg accgcgactt tcgcggcggt ctcgagacct tgcagcggct
     5401 gtccagcgag ctaccggtgc ccgtgatcgc aaaagagacc ggttgcggcc tatccgcccg
     5461 cgcaggcgca cgcctgaaga aagcagggat tcgacacgtc gatgtctcgg gcgccggggg
     5521 cacctcctgg gtggccgtgg agacgcaccg cgcgcgcgac gaacggaaag ctctcggcga
     5581 gaccttttgg gattggggaa taccaaccgc cgcgagcgtg ggctcgttga ggcctttcgc
     5641 gttcgacacg ctgatcgcaa cgggcggcgt gcaaaacggt ttggacgcgg ctcgagcgat
     5701 cgcgctcggg gctcacttgg tgggcatcgc gcgaccgacg ttgcaggctc tggatcgggg
     5761 cggcccgagc gaagccgaag ccttcttgcg tcgcgtagag agcgaactga agacggcaat
     5821 gctactcacc ggctccccca acttggatgc gctacgcgcc gccccgcgca tccttcggag
     5881 tgagctgcga gattggttgc acggagcccc ggacacgtcc ggcggctgag acggtcccgt
     5941 tgagctccac ccaccgagcg tcagcgcgga cggcgggggg tggcgggcgg cggcgggtgc
     6001 gagtgcgcga cattggattc acgggcttgc cacgccaagc tcgaagcctc ctggtagagc
     6061 tccttcgccc cgggaaagtg ctcgttctgt cgcatgatct gaatcaactg ctgcatcgcc
     6121 gcgccgggcg gcaagcgctc ctcgcgcagg ccgagcttga tgcgcagcac gatcacacgc
     6181 tcatcccctt cggggtaact cgcaagcgcc gagcgagcac ccgcaaaatc ggaagcatcg
     6241 atgaggcgat cgatctcagc gggctcgatc gcactgacag tggcatccat tgcggagccg
     6301 agactatcag atttccgacg ccgggcggca gcgctcgtgc aggacgctcg ctcgcgacca
     6361 accccagctg tccaaggtcc cccgcccctc ggtacacgag ttgccccaag tgctcccacg
     6421 aggctcgatg taagagcata tccgtcacgc caccccgcca attagcgagt tcgcgtgatt
     6481 gacaggccgc gcggaggggg tgttactctg cgagaggtga cgagggcgcc gtcaccgccc
     6541 gggctgggac aggctggaat gtaacgacga tgagcgaaga gaaccgccga cgttcggagc
     6601 acagtgatgc tcccgcaccc gatttacgga tcgaacggga ccagtttttg cggaagttct
     6661 cgcgcggagc gcggctaacc gaagacttct tgcgcgctta cgaggagctc gagctgcgtt
     6721 gcgccgccct cgagtcggag aacggcgtgc tccggacgca ggtcgaggcc ggcgacgcaa
     6781 tccgcgagct gctaaacaag attcagcggc tggagcagga gaaggacgag ctcctgtcgc
     6841 gcttccgacg cgtcgaggcc caaagctcga ccgtcagcgc gcgcgtacag gaggtggagc
     6901 acgagttcgc caacctggcg aacctgttcg tcgccagcaa ccagattcac tcgagcctgt
     6961 caccccgcgg ggtcacccgc cgcatcaagg aggtgctcgc gcagttcgtg ggagccgagc
     7021 gctactcgat gtacctcgtc aatcccgagg ggacggagct cgtacccatc gcgttcgagg
     7081 gcgttcccgc ttccgaactc gcggcggtgc gcgtctccgg cacgttcttg ggccagattt
     7141 tcagctcacg cactgctttc gtggacgaag atcacgacgc gagccagggc accttcgacc
     7201 ggccggcagc cgtgattccg ctatccgtgg acgagcgcgt ggtgggagtg atcgccatct
     7261 tcgcgacgct atctcagaag cctcgtttcg acacggtgga cttcgagcta ttcaaactgc
     7321 tcggagaaca cgcggcagcc gccctggtta gcgctagtct attcgtccaa gcggaccgca
     7381 aactgccggg actcgaggca ttcctcgatc tgagcgtcta ggacttttga gcgtacagaa
     7441 atatggccga atactcctgc ctgatcgttg aagactcgcc gatgatgcgc cagctcttgg
     7501 tgttcgcttt ggcgcgggtc aagaatctgc gagttacgga agcggacgac ggggtggacg
     7561 gcttgcgtaa gcttgcctcc gctcgctacg acatcatcgt caccgacatc aacatgccga
     7621 tcatggacgg cctgaagttg gtcaagcgcg tacgcagcga tccggtgcac aaggataccc
     7681 ccatcgtgat catcaccacc gagggctcgc aggaagaccg gcagcgcgcc ctgcagctcg
     7741 gtgccaacgc ttacatcacc aagccgattc aggctccgca ggtgattgcg acggtgaagg
     7801 agctcctgcg catcgagtga gtggcgagcc gggctgtgtc atggcacaaa gccgcggcat
     7861 gggacgcttg cgcgcagaga tcattcggga gcacatcggc gttcgggagc acatcggcat
     7921 tcgggagcac atcggcgttc gggaggaagt cactcagtgc ggccttggct gaagatctgc
     7981 ggaataacgt gcgacgagga cgcggatcta gtcgtcgacg ctggcgccga tgcgattggg
     8041 ttgaacctgg ttccgcgctc caaacgctac atcgatcgca agctggcgcg acgcctagct
     8101 gaccgcgtgc gcggcaagct cgagattgtt tgcgtagtgg ccgacgagag cttgggcagt
     8161 cttcgtgagc tgcaaaccga gctcgcgccg gactggcttc aattgcacgg cgaagagccc
     8221 gcagcgtggt tgaacgagct gccccggtct ttcaaagcgg tggggctcgc tgagccgagc
     8281 gatgcgctcg ccgcggacgc ttatccaggg agccgcctgc tggtggatgc gaagagcggg
     8341 ggatctagtg gcggcacggg cctgagtttc gactgggctt gggcggccga gctgtgccgc
     8401 aaacgagacg tgatcttggc cggcggtctc aaccccgaaa acgtggcgca ggcctacgcg
     8461 gccttgcacc ccttcgggct cgatgtggca agcggagtgg attcaccggg caacccccga
     8521 cgtaaggatc cgctgcgtgt cgcgcagttc gtggcgcgtg ctcatgcggc ggcgcaataa
     8581 acttacgccc atcttgagcg gcgcgccggg gtgggttcac gctcgttccg cgagcatgtt
     8641 gctcgggctg ttggcctcac tcgctgtgcc gttcgcgtcg tcgtgcggcg gtcctaattt
     8701 gcgtggggcc ctcgcggtgg accgcggaac ggagccgcgc gacggtgagc tttcgagcta
     8761 ctcggttcgc agctgtagcg acgccaccgg ggcgccggcg tccggcgcgg gccctcgcgt
     8821 ctcccatcaa cgctcgcaga aacgggacat cctggtggag tttcgacctc gctactccag
     8881 cgtcgtgatc gtgaacactt acgcggaggc caatgagcag gtcttcgcgt acgtctcgga
     8941 tgacgcccag cgagccgcaa tcctgcacga gtatcgaatt cctcagcccg acaatgcctc
     9001 gggcggacgc ttactactgt tcgaccgcta caccttggcc aaagacggag acactttccg
     9061 gcgcacgatg ggcagcccca tcgccacgtg cacgctggtt cccatcgcga ccgatttgcg
     9121 ctgagcgcgc cgctgcccgt tcggcgcgac ttgctcctaa gagtacttcg gcagttccgg
     9181 caggttcacg ctcaggggtg taacctggat gccggttgcg cccgtggcaa cgatcgttgc
     9241 ttgcgcgtaa ccgccgcccg gcgcctgacc cacggagccc acgctgatga tttggacgtc
     9301 ggctacccgg cgctcgaact gcgcatgact gccgccgcac acgatcacgt cgcccggctc
     9361 gtctcccaac agcgcgatca gctcgtcctc cgtcatgtcc atcgtgaagc actcggtcgg
     9421 atcgagcggc gaaccgtgca ccaccatcaa ggtgtgaccg gactcgagcg gcaggcgcgc
     9481 gatcgtaggc agcttaccca aacgagtaat gatgagctcc ccgagttcgc cttggatctg
     9541 ccgaaagcgc tcgatgcgct cgcgctcgtg ctccgtggtg gccagcaact tcgaaggatc
     9601 gatctgcgag agggcccgat cgctcactcc ttgaacgcaa agcgcgcgat gacggacgag
     9661 tgtcttccac accttgagcg gctccgggcc ggggaaaacc agatcgccgc acgccacgag
     9721 ctgatcccag ccataaccgt cagcctcctc taggatcgcg tcgagctcgc gggcatgacc
     9781 atgaatgtcc gagacgcaga gaaaccgcat cgctcgggct gatacgccgg agtgtgctcg
     9841 agaacaaggc cctccgagca ccggcctcac gaccaagtgg cgttacgggc ccgcaaaact
     9901 ctggacatcg ggactggctg cctcacctcc gtgacggcgt tagaacagca cgagacgtgg
     9961 aaacgacccg tgctctgctc gccttggcca atcgtatcga tcgggatcgc gcggcaatct
    10021 tgcgcgcgga attcgggccg agtcccgagc gtgttccggc agtgttgctg ggcgccgcct
    10081 tcccggccct cactccgagc tccgaattca aactgcaagc cgtggagcag ctggcgcgac
    10141 aagggctgcg agtcgagagg cgccgccgcg atctggtacg tgacgtgctt tccgcggtgc
    10201 cgggcgacgc ggagccggag gcctttggac gggcgctgcg gcgcttcgtc tgggcggagc
    10261 gggcacgcat cgcgctccgc gagttgttgc cgctcgagct cggcggagcc tcgatcgagg
    10321 tcaccgcacg ggagctgagc gtcctctcgg aagccgccct ggagctcgct ctgcgtgagg
    10381 ccgctcggac gatgcgctcg cgttacgggg ccccaatccg ctcggacggt ggagagtcga
    10441 gcctgtgcgc gtttggaatg ggcaagctct ctggacacga gttgaactcg ggctccgacg
    10501 tcgacttgtt gttcgtctac gacaccgatg atggtcagag cgcgctgacg ctgcacgagc
    10561 attggtcgcg cgtcgtccgt cgtgcggtga gcaccatcga ttcgctgact gaggatggtc
    10621 aaatctggcg cgtggatctg cgactgcgcc ccgagggctc ctcgggcccc atcaccaatt
    10681 cggtggctgc caccgagcgt tactacgaaa cgtgggggcg attgtgggag cgagcggcgc
    10741 tgctgcgctc gcgccccatc gcgggtaact tggagctcgg cgcgctgatc gagagggaga
    10801 tcttcacgcc attcgtatac cgccacgcgg tggatcccgg gctggcgggc ccgctctgcg
    10861 ccatggtcga gcaagggcgc aacgagctct cgcccgatcc gactcgagac ctgaagctgg
    10921 gtcccggcgg catccgcgaa gcagagttct tcgtgcagat tctacagctg gtttggggtg
    10981 ggcgggatcc caacctgcgc gtgcccggca cgctcgcggc gctggcgcgc ctagccagcc
    11041 gggggctagt gactgaccgc gagtcgcgag ccatttcctc cgcctacgtg tttcttcgcc
    11101 tgctcgaaca tcgcgtgcaa tgggcgagcg gactgcaaac tcacctgatg ccggaccagc
    11161 ccgccgatct cgataagctc ggacgcacgc tgggttatcc ggacgcggcg cccatgctgg
    11221 cggagctctc tcgcgctcga gccgcaatct cggaagcctt cgcgggggtg cttcccggac
    11281 gcgtgagccg acgccagcag cgctcgccgt tcttcagctt actctcggcg ctgaacgatc
    11341 cagtgcgctt ggcgcgagaa gcggagcgtc tattcggaag cgccgacttg ggcgagcacc
    11401 tcgccgcgct ctcgcggaga cccgatggtc tgcttggtga gttgacgcag gaccgagctc
    11461 ccggcttggc cggacgcctc ctcgaagcgc tgtcgaccag ccccgacccc gagcaagcgg
    11521 cgcgctatct gcggtccttc ttcgcgcggt tggtgacgcc aatggcttac gtgaccgcgc
    11581 tcggcgaaga tccacgcatg ctcgcgcgct tcgtgacggc cctgagctcc agtcagttcg
    11641 tcggagacgc gtgcatctct cgccccgagc tcgcggacat cgtgctgttc ggtgaaggta
    11701 aggtgagcga ccccaaggcc gccttggagt tggagctcga gagtcagccg atcgatgacc
    11761 ccgaggagcc agacgaaggc aaccaagcct tcgtgacggc gctgtgtatg gcccggcgcc
    11821 gcttcatggt ggaagtcgcg gtcgcggatc tggcaggcac gctgagtgcc cgtagcacca
    11881 cacggctatt gtccgatctg gccgatgagg tgctacgccg agccgcagag cgcgtgctgg
    11941 gcccgggggc gcctgggctc gcggtgatcg cgctcggaaa actcggcggc agagacatcg
    12001 gctacggctc ggatttggat gtgctgttcg tctacgaccc gaagcgccaa agctcgcgcg
    12061 aagaggcgca cttgttctac acgcgggccg cgcagcgcat tctgaggctc atttccgagc
    12121 ccagcgtggc aggcccgggc tatgtcttgg acacgcggct ccgcccttcg ggagcacacg
    12181 ggctgctggt gacttccctg cccgccttcg cgcgctatca cgggatcgag ctcgaggatc
    12241 ccgaggagac tgcgaccgcc acttcatcgg gtgccgcctg ggaacgtcaa gcattgttgc
    12301 gggcgcgagc ggccgccggc gacgcggcgc tcggagagag agtggtcaag attgcggaac
    12361 aagcagccta cgagcgtgga gcccccccgg tcgaagaaat gcatcgcctg cgtcaacgca
    12421 tggagctcga gctcggccgc gaacgggcct ggcgcatgga cttgaagagc ggccgcggag
    12481 gcctactgga cgtggagttt tgcgtgcagt ggttgcagat gcaccatggt gaggacttgg
    12541 gggtgcgcac cacggacacc tccgaggcgc tcgaggctct ccacttgttc ggctacttgt
    12601 cgcgtccaca cttcgacgca ctccgcgacg gctacagctt cctgcggcgg ctggagcaga
    12661 gaatccacat cctgtcgggc atcgcgtcgg aggtgatcga tctgacgacg cgcggcttgc
    12721 ctcagctcgc gcgtcgcatg ggttttcgcg acggccctgg cccaagcgcc aaggaccagc
    12781 tgctcgcgcg atacgcgagc gttacgcaga gcgtgcgagc agcctacctc gaagcgctcg
    12841 agctgagctg attcgctcgt gtggcatggc ttacgcggca cgtcctgcgc gacgcagctt
    12901 gcgagtcatg gccaaagcga aagcgccgcg ccgcgagacg atatcttccg agctaagtct
    12961 tgatggtctg gcgcaaggcg cgcgcggcct cgatcgcacc gtaggtgaag atgatgtccg
    13021 ctcccgcacg acgcaagcaa accaaagact cgagcagaca gcgctcgtag tcgagcacgc
    13081 cccgttccgc cgcgaacttg agcatcgcgt actctccgct cacgtggtac gcggcgacgg
    13141 ggagcgtgct gtgctcacgc acgtagcgga tcacgtccgc gtaaggcagg cccggcttga
    13201 ccatcaagca atcggcaccc tccgcctcgt ccagctcgat ctcgcgcagg cattcccgca
    13261 cgttgccggg atccatttgg taggtcttct tgtctccgct gcgtggcgcc gagtccagag
    13321 cattccgaaa aggaccatag aaccccgacg cgtacttcac gcaatagctg caaatcccaa
    13381 catccgtgta acccgcctcg tcgagcgccg cgcggatcac cccgactcga ccatccatca
    13441 tgtccgaagg tgccacccaa tccgcaccgg cttcggcttg ggccaccgcc atccccgcga
    13501 gcagcggcaa ggtctcgtcg ttgtcgattc gaccgtcgat cagcacgccg tcgtgcccgt
    13561 cggaagaata ggggtcgagc gcgacgtcgg tgatcacgag cagacccggc aaggcttgct
    13621 tcaacgcacg cactgcgcgc ggtagaaggc ccgcgcggtt caggctctcg gtgccccgcg
    13681 catctttcac tccctcgggc aatgccggga acagggccac gccggcaacc cccaaagcat
    13741 gagcctcgcg tgcggtctcc aacaacagat cgatcgagag tcgagcctga ccgggcatcg
    13801 aggtgatctc ggtgcgttgg cccgtgccct cttgaacgaa tagcggcatc accagatgcc
    13861 ctgcggtcag atgcgtctcc cgaacgagcg aacgaatggc ctcggtgcgg cggttgcggc
    13921 ggggacggtg aacgagctga gttgtttcgc tcatggggag cctttgcatg atcctagcct
    13981 tttgcgcgtc tagcctgatt ctgtgggtac gaactgatgc ggtcgggata gcccgagacg
    14041 tcggtataac ccgatgttgc cggtataacc tgatgatcga tgcgagcccg ctcgaatcca
    14101 gtgcaagtgg cggcagggtc ttctatgctt acgcccgagg agcacgatgt cgagcaagaa
    14161 ttcgagcgag aaagcctatc tggtcatcac cgccgtaggc ccagatcggc cgggaatcgt
    14221 gagcgatcta tcgcgtgtaa tccacgcggc gggggcaaac ctggaggaca gccgcatggc
    14281 ggtgctgggg ggcgagttcg cgctgttgct tctcgtctcg ggcagcgaag ccgagctttc
    14341 gcgcgtgctc gcggacgcgc agagccaaga gagcacgctc gggctgcgct tgatgagcaa
    14401 acccacgagt gggccgcgcg ccgctcgcaa tgtactcccc tatacgattc gcgtgacagg
    14461 tctcgatcgc ccgggcatcg tgcagtccgt cacgggcgtg ctcgcgcatc accgcgtgaa
    14521 cgtgcgttcg ctcgaatctc gggtcacgta cgccgccgag agcggcaccc cgctctttac
    14581 gttcgaggcc gagctcgagg ttccgtcggc ggtcgtattg gccgccttgc gcaaagagct
    14641 cacgacgcga gccgacgagg acaacctcga tctcagcctg gacgcgcggg tttgagtgtt
    14701 ggtgcgtcga cggcggtctg cggcgcggcg gcgtaatccg aagggggaga cgacgtgcgc
    14761 cccggcatca ccggcacgcc caagaactcg aagaacttac gaacccgctt gagcggtttg
    14821 tcagcgcgtt tgagctttcg ttgtagtcga ttccactcgg cgactttctc gtgatagacg
    14881 cgcgtggctt cgacgtaacg ctggtggagc tcgtacaacg ccgagggatc gttggcaccg
    14941 tcgagctcgt ataggcaatc ggtagcctgg atcagcttca agcggcgtag ctttccggag
    15001 cgctcgaacc gggggtcgaa cgggaagatc cggcaaccga gcggccgtgc atcgtagatc
    15061 ccgcagcggt cctgggcatc caagtaacgg caacctccgc gctggtgcct gagcaccatg
    15121 acgcgcttgc cctgcggcag aatcacgaag gcctccggct cgtcgtccat atcgattccg
    15181 tttcgatcca cgaaacgtac gacatcggcc accgcgtcac cggtgtggtc caggatgctc
    15241 cggagatccg tgtccgttaa agggagcagc ggttccttgc agcagttgcc gcaacccgtg
    15301 caccggaatc gaagcaggtc ctttgcggtc ttcccgcccg ccgtcttcac gcctgcagtc
    15361 ttcacgcgcc gatgttcccc tcggctagcc tccgccggca agcatcaaaa cacggacacg
    15421 cccatcccag caggtgcgag tggtcgttgg ggaaccatcc cgcgcagaac tgccgggtga
    15481 gatctccccg gaaggacgct cgaacccatt ccaaagagtc cgtccgacac agagctccgg
    15541 tcgcgggccg catggccgag cccccgctgc caagacgtct agccgcgacc cccgccgcga
    15601 cgacgccgga gtgggcatgc attcggggaa tgctcgtctc gaggcgctgg aaatgtgcga
    15661 gacttgcgac gatgtccctg aagatcgacc aggaccactc gcgctttcgc agcatcgttc
    15721 gcggccggat tcgacagaac ttgaggcgtt acatctccca gggggaaatg ctgggtcgca
    15781 aggggaaaga cctcgtcagc atcccgattc cgcacatcga tattccacgc ttcgtgttcg
    15841 gtgatcgtca aaagggaggg gtcgggcaag gtgacggcca agtcggggat ggggtcggcg
    15901 gcgacgaagc cgaacaagag ggctccggac aagccggcaa agacgcgggg gatcacgtcc
    15961 tggaagtcga cgtcaccctc gacgagctgg cggacattct cggggaagag ctcgagctgc
    16021 cggcgatcga aaacaagggc aagagccgca tcatcgccgc caaggatcgc tacaccggca
    16081 ttcgccgcgt cgggccagag tcgctgcgcc acttcaagcg cacctatcgg gaggcgctga
    16141 agcgcgcgat cgcgagcggc gtatacgacc ccggcatgcc gctggtggtg ccgcaacccg
    16201 aggacaagcg ctttcggtcg tggaaagaag acctcgagcc cgtcgcgaac gcggtgattc
    16261 tgtacatgat ggacgtgtcc ggctcgatgg gtgacgaaca gaaggaaatc gtgcgtatcg
    16321 agagcttttg gatcgatacc tggctgcgtc gtcaatacaa ggggctggag agccggtaca
    16381 tcatccacga cgcggtcgcg cgcgaggtgg accgcgagac cttcttcaag acgcgcgagt
    16441 ccggcggcac gatgatctcg agcgcttacc ggctggcggt gcagatcatc gaccagcact
    16501 acccgagcga ggagtggaac atctatccat tccatttctc cgatggtgac aactggtcga
    16561 tggaagacac gctcacctgc gtggagctgc tgaagaaaga gatgctgccg aaggtgaaca
    16621 tgttcgctta cgggcaggtc gagagcccct acggttcggg gcaattcatc aaagacatgc
    16681 gcgagcattt cggtgcggac gagcgcgtcg tcaccagtga aattcgcgat cgcgatgcga
    16741 tcgtctccag catcaaagac ttcttgggca aaggtaagta atgtctcaat tcgcaacgaa
    16801 cacgtcgctg ccgcgttacc tgcgcgtcga gcaggagcgc atcgagaaga ttgcacagaa
    16861 gctcgggctc gatttctttc ccgtggtgtt cgagatgctc agctacgatc agatgaacga
    16921 gatcgcagct tatggtggct tcccgaaccg ctacccgcac tggcggttcg gcatggagta
    16981 cgaacggctc agcaagagct acgagtacgg gttgtcgaag atctacgaga tggtgatcaa
    17041 caacaaccct tccttcgcgt acctgctcga gggaaacagc ctcaccgacc agaagctcgt
    17101 gatgtgccac gtgttcgggc acgtcgattt cttcaagaac aacttctcct tccgcgccac
    17161 cgacttggac gccggagatc cggtgacgga tccgatggcc aaggggggcg ccaactacga
    17221 tccgaatcgc aagtggatcg acaagatggc caaccacggc gcggcggtgc gccggatcgt
    17281 ggatcgctac ggcatccagc gggtcgagga gttcatcgac aactgcctct cactcgagaa
    17341 cctgaccgat ccgtggtcgc gcttcgtggt acgccgacgg gagcgcgagg aggaggaaga
    17401 gccgaaggaa atcgaggtcc cgcgcttgcg cgcgaaggac tacatggagg agttcatcaa
    17461 tccttcagac tacatcgaga aggagcgcaa gcggctcacg caagagcgcg atcgcgccaa
    17521 gggccgccac ccggcgcacc ccgaacaaga cgtgctgttg tttttgatcg agaacgcccc
    17581 gctcgagcgc tgggaacgcg caattctcag catcgtgcgc gaagaagcct actacttcgt
    17641 gccgcaaatg cagaccaaga ttatgaacga gggttgggcc tcctactggc actcgcgcat
    17701 gatgaccgag caagtctgcg attggagcga gatcgtcgat tacgccgaga acaacgcggg
    17761 cgtgatggca accgcgaacg ggcgcctgaa tccgtacaag ctcggggtag agttgttccg
    17821 cagcatcgaa gaccgctgga atcgcggtca attcgggcca gagtgggagc aatgtgatga
    17881 tctcgacgtc aagcgcaact gggacctgcg gctgggcctg ggccggcaga agatcttcga
    17941 agtgcgggcg ctatacacgg acgtgacgtt catcgacgag ttcttgacgg aggagttcgc
    18001 gatcgagcac aaactcttca cgttcaactg gtcgaaccgc aacgagcgct tcgagattga
    18061 gtcgcgggag ttccgccaag tcaaagagaa gctgctctcg caactcacga acttcggcaa
    18121 cccggtcatt tcggtgctgg acgccaactt cgataatcgg ggcgagctct tgttgcgcca
    18181 cgatcaccgc ggcatcgacc tgcgctccga ctacgccaaa gagaccctga ccgcgctgca
    18241 ccgagtgtgg cgacgaccgg tgtgcttggc gactcaacta gacggcaagc gcgctctgct
    18301 ccggtacgac gggaaggaac acagctcgcg ccccgacgcg ccgtgatgtt tgacctcacc
    18361 gcacccgcgc gatcgcgaac tgcgatgcga tcactccctg cggcgccatc acagcgcgaa
    18421 gcgccatcac agcgcggagc gccatcacag cgcggagcgc aggccctcct tctagtgact
    18481 gcgatcagtt gggcctgcgc gggagcacca gcgggtcagc cgcaagctcc ggttgccgtc
    18541 cgttcgaata tcaacacccg cgatagttcc ggctctctct cgccggaagc tgaagatgct
    18601 gcggcgccgg gcggagaagc agcgcgcgaa aacccgcctt cctccggccc ccctgacggg
    18661 tcaccgcacg gagcagctcg aacgcccggt ccagccggga gcgccaacgg gccgggtaca
    18721 cgagcagtgc cgggcggact atcgcggggt ggcgactcgg agctcgaacg cgctctgagc
    18781 gcaggcgacg cggcctactt cgatggagaa ttaccgcgag cacgcgctga gtacgaacgc
    18841 gccctcaagt tggcgccgca agacctggcg ccgcagctcg gattgctgcg ggtcgagctc
    18901 gccgggctgg acgtgccgct cgattacgcg gcggccccga acgacgcacg gctgctgggc
    18961 ttattgaagc gcttggatat gttgaaagct cgcgacccag gctacgggcc taccgagctc
    19021 gagcgcggac gggtgctctt gattttgggc cgcgccgctc ctgctctgca ggcgctcgag
    19081 cgcggcgtcg ccacgcttcc gaaggatccc gaggcccact cggcgctcgg cgtcgccctg
    19141 ctcgcgaccg gccgagccgc agaggcgttg ccgcgttttc gcaaggcgta cgagctcgag
    19201 ccggccaacg ccgagcgagc ccgtgcgcta ggaacggcgg agctcctgac cggcaacgtg
    19261 gaccgcgcca tcgcagccta ctcgcgagct gttgccctgg cgcccactga tgcccgggcg
    19321 cacagtgact tgggcagcgc atacttggct caaaatgcgc ccgtgcaggc cgtgcccgag
    19381 cttcagcgtg cggtcgactt ggatccaacc cgggcgacat ttcgctccaa tctcgcgtat
    19441 gcagcgctgc tggtcgggga caaccccgca gccgccgaac aggcccgtca cgccatccgg
    19501 ctcgatccga agctcgcgtc agcgcacctg aacctgggca cggcgttagc aaggcaaggt
    19561 cgttacgacg acgcggaacg cgctttccgc agggcgctcg agctcgatcc aagcgatccg
    19621 cgcgccaaaa ccaacctaga ggagctgcaa gccctccggc gacagaagcc ccaatgagct
    19681 gcccgttcga gagctcagac tgccgcgacg ctcatgctaa gctgccgcgc ccgagagcga
    19741 tggaacctgc gcggcccacg actggagcgc ttcgcaatta gccacttcgc agtaaccact
    19801 tcgcagttga ttgtgccccg cgacgttgcc gagcctccaa atcgagcgcg gcgcaagtga
    19861 gctgagccac ccaagtagac cgcgcgcaac gtatgcaata gccgtgcgct gcgcccaatg
    19921 aaccgagtgc aggagacgta tgaaaggaat cgtactggct ggaggtagcg ggactcggct
    19981 ggacccggtc acgcgcgcag tgtcgaagca gctgctgccg atttacgaca agccgctcgt
    20041 ctattatccg atctcggttc tgatgatgtc agggatccgc gagatcctga ttatctcgac
    20101 gccgcacgat ttgccgctgt ttcggcgtct actcgggact ggggaggaac tcggcctgac
    20161 cttcgagtat gccgagcagg ccgccccgaa cggccttgcc gaagcattca tcattggcaa
    20221 gcagttcatc ggctcggact cggtgtgcct cgtgctgggc gacaacatct tttacggtgc
    20281 cgggctcgtg aagaccctgg tcgacgcgcg cgcccagctc gatggggccg tgctgtttgg
    20341 ttatcgggtg gaggaccccg agcgctatgg tgtggcggag ttcgcgcccg acgggacgct
    20401 gctcggaatc gaggaaaagc cgcgcgctcc caagagccag gtcgcggtaa cgggtctcta
    20461 cttcttcgac aaccaggtcg tggagatcgc cgctggtttg aagccgtcag cgcgcgggga
    20521 actcgagatc acggacgtca acaacgccta catccaacag cgccgcgcga aaattcaggt
    20581 actcgggcgt ggctttgcgt ggctcgacac cgggacccag caaagcatgt tggatgccgc
    20641 gcacttcgtt gcgacactgg agcgccgaca aggcacgtac atcgcgtgtc tcgaggagat
    20701 tgcataccgc tccggttata tcgacgcctc acggctgcgt gcgctcgcgg cccgatgcgg
    20761 caagagcgaa tacgggcgct acttgtatcg tttgctcgac gaaaaggcca cgccgtgaca
    20821 gaaggggccg cgcgatgcaa gcaagcgacc tgcggctgag tggtttgaag ctcatcagac
    20881 ctcaagtttt ccgagatgag cgcggttttt tcgtcgagac ttaccacgca cagcgctacc
    20941 gagacgcgaa catcggcgtc gagttcgtgc aggacaatta ctctcgctcg gcgcgcggca
    21001 cgctgcgcgg actgcactac cagtcgagcc ctgggcaagc caagctggtg cgggtgacgg
    21061 ccgggcgcat ctacgatgtg gtggtggata tccggccgca ttcgccgact ttcgggcaat
    21121 gggagggctt gcacctagac tcagaggagc atctgcagct cttcatcccg gttggcttcg
    21181 ctcacggctt ttgcgccgtt tcggaggtcg cggacgtgca gtacaaagtc agtaacttct
    21241 actcgggcgc gaccgaatgt ggcttacgct acgatgatcc cgatatcggc gtcgtctggc
    21301 caatcagtga tccggtgctc tcggagcgag accggaacgc ggagagcttc cgcgagtttc
    21361 gcgcgcgagc gctgaaatga aggtctggtt gtgcggagcg cggggtatgc tggggcgcgc
    21421 catcgaagct cgcctgcagt cgctcgcggt ggcgtgcgtc ccgaccgaca gcgagttcga
    21481 cgtgactcaa ggcgagcccg tgatcgcgtt cgcgcgccaa catgccttca ctcacatcgt
    21541 gaattgtgcc gcttacacgc gtgtcgacgc ggccgagtcg gaacccggga cggcgcacgc
    21601 ggtgaacgcg ctgggggcca gtcatctggc ctccgcggcc gcggcctgcg ccgctcactt
    21661 gctgcacttc tcgacggact acgtgttcga tggggccgcg cacgagccat atcgagagga
    21721 agcagcctgc gctcctcgta gtgcctacgg tcgcaccaag ttggaaggtg agcggctggt
    21781 cctcggcacc gcccccgctt cgatgcgaga ggcccgcgcg gtgcacgtgg tccggacctc
    21841 ttggttgttc ggccctggtg gcaagaactt cgtcaccacg atgctgcagt tgatggcgga
    21901 acgcgaaacg ctgcgcgtcg tggcggatca ggtcgggtgc cccacctaca cgtacgactt
    21961 ggcagacgcg agtttggcac tagcagcggt cgggccgagc gcccgcgccg cagccccaag
    22021 cggcatttat cacttttcca acgccgaacc cgtgagctgg tgcgggttcg cgcaggcaat
    22081 ccacgagcgc gcgcttggct tgggattccc aattcgcgcg caggcgattc aacccatccg
    22141 aacggaagag tatcccctgc ccgcaacgcg ccccgcttat tcggtgctag acacgagcaa
    22201 aatcagcggt cagctcggac gagcgcctcg tccgtggcga gccgcgctcg acgaatacct
    22261 gacaaccctc cgaagcagct gatgtcagcc caaagaccca ctcacaatct gctcgtcacc
    22321 ggcggctcgg gcttcattgg ttcggcgttc attcgctggt tgttcgcgca gccttcgtac
    22381 agcggcaaga tcgtcaacct ggatctgctg acatatgccg ccaacccgga caacgtgtcg
    22441 caagttccgg ccgatcgtta taccttcgtg catggcgata tcctggacgg caagctcgtc
    22501 gaggaaacct gccgtgccca cggagtcgac acggtcgtcc acttcgccgc cgagagccac
    22561 gtcgaccgca gcattcatgg cccaggcgtg ttcatgcaca cgaacatact cggcacttat
    22621 cgcctgctgg aggtagtgag agcgctgccc ggcgttcact ttcaccacgt ctcgaccgac
    22681 gaagtgtacg gatcattggg cccgcagggc tatttcaccg aaaccactcc ttatcagccg
    22741 aactcgccgt attcggcctc caaggccgcg tccgatcacc tggtgcgagc gtacgcgcat
    22801 acgtacggcg tctcggtcac gatttcgaac tgcagcaaca actatgggcc gtatcagttt
    22861 cccgagaagc tgatcccgct gatgatcatg aatctgctgg agggcaagcc attgccgatc
    22921 tacggcgacg gctcgaacgt gcgcgattgg ttgtatgtcg acgaccacgc ggaagccatt
    22981 tggttgattc tgcaacaggc caagagtggc gagaccttca acgtgggtgg caacgccgag
    23041 tggcccaacc tgcagctcgt ccacaagctg atggatgtgg tggccgaact cacggggcga
    23101 agtcgcgagg cgctccagaa gcagatcacg ttcgtcgcgg atcgcccagg tcacgatcgt
    23161 cgctacgcga ttgattgctc gaagctccaa agccagctcg gctggacgca acgtgagaac
    23221 atcgacagcg ggctgcgcaa gacggtgcgc tggtatctag acaacccgct ctggctcgag
    23281 cgggtgcgca gcggagctta tcgggagtgg atcgatcgca actacgggaa gcgtcagtaa
    23341 cgcttcgatt cgcggcaagg acgcgcttgc caccggccag tttccccgcc gatggagcgt
    23401 tcgcggcaaa gcaccgcggt tttcaacggc tctggatcat accgctcaag tgactttatc
    23461 cactgcacat gctagtcgca acggcgactt ctgctttgga tgacgttatg aattcgccga
    23521 gtatcgccgc gataatcgtc aactggaact cacgcgacga tgtcgtggac gcggtgcgca
    23581 gcctgctcac gcaggctgat tccgacgtgg atatcgtggt cgtcgacaat ggttcgaagg
    23641 atggctcggt ggaagcgctg cagcagtttg cgccgcaggt gcgcgtgatc gagaccggcg
    23701 cgaacttggg tttcgcggag ggatgcaacc gcggaatcga ggccagcaat tcggactggg
    23761 tgttcattct caacaacgat gcgatcgtgg cccccggcgg gatggcgcgg ctgcgcgcgg
    23821 cggccgccgc ggcgcccgag gatttgggga tgctgcagcc ctgcatcgtg ttccggacca
    23881 acccggatca agtgaactct tcgggggtga tcgtgttcag caatggagcc gcgcgcgacc
    23941 gcgacttcgc ggcccctgtc agcgcgaccc aacagggctc ggaggtcttc tgctgcaccg
    24001 ccggtgcggc cctctaccgc cgcagcatgc tgcagcgagt taagcgaccg acgggctatt
    24061 tcgatcgcac cttcttcatg tacttcgaag acgtagactt ggggtggcgc tgccgactcg
    24121 ctggctatcg cgccgtctat tttccggagg tggtcgtgct gcaccgcttc caggcctcgt
    24181 cttcacgtcg gggccgtcat ttcgctcgct cacacagtca agccaaccgc gttcgcagcc
    24241 tgatcaagaa cgcctcccca cggatgttgg cgcgctccat cccgcgcacg gtcagtgacc
    24301 taacccagct cacgatgcat gaccctcgcg cgctcggtcg tctgctgcgc accctgccgg
    24361 agctattgca ggagcggcag gccatcgctt cacttcgcca ggtagatccg gctcggatcg
    24421 agaaggattg gatggcgaac aagcattttc cggcccccaa gcccgacttg ccggcgccgc
    24481 cgaaggcgag ttcaggctaa tccagccgcg ctcgccggct cttgggggca gtcccggcgc
    24541 ttccgacttg ccgagcgcgg gttccgatcg cacagcttgc cgcgtgatcc gcttcgactc
    24601 gatcaacaag ggccacggcc gtcagctgct gtttctagat gcctccgcca gcgtcaatag
    24661 aggtgagaag gtggggctcg tcgggccgaa cggctccggc aagaccacgc tgttccgcct
    24721 gatcatcggg gaagaacagc cggataacgg caatgtcgtg ctcgatcggg gcgtgacgat
    24781 tggctacttc agccaataca tcggcgacat gcgcggcaag agcgtgctgt ccgagaccat
    24841 ggcgggagca ggcgaagtct cgcgcgtggc cgaggagctg caccaactcg agcatgcgat
    24901 gacccacgcg gcgggggacg aattggagaa ggccgtcgag cgcttcgggg aggtgcaagc
    24961 acgcttcgac gaactcgacg gctacgggct cgaagccaaa gcgcgcgaga ttttgaccgg
    25021 gctcggcttc cgaccggaga tgattgacgg cgacgtcggt cgcctctcgg gcggctggaa
    25081 gatgcgggtc gggttagcac gcatcctgct catgaagcct gacctgctgt tgctggacga
    25141 gccgacgaac cacctcgatc tggagtcgat cctgtggctg gaggctttct tgggggcgtt
    25201 cgagggggcc ctcgtgatga cctcccacga tcgcgagttc atgaaccgcc tctgctccaa
    25261 gatcttggag atcgatggcg gcgagctgaa gacctactcc ggcaactatg atttctacgt
    25321 ggctcaacgc gagctcgacg cggcccaaaa ggaggctgca tttgcccggc agcaggccat
    25381 gctcgccaaa gagcaagcct tcatcgacaa gttcaaggct cgcgcgagtc acgcggccca
    25441 ggtccaatcc cgggtcaaaa aactcgaaaa gatcgagcgc gtcgagccac cgcggcgacg
    25501 aaaggtcgtg gagttcgatt tcccgtcgcc tccgcgctcg ggtgaagatg tggtgaagct
    25561 cgagggcgta cgcaaagcct acggcgaaca cgtcatctac tccgagttcg actttttgat
    25621 tcgtcgtcgc gaacgctggt gcgtgatggg tgtaaacggc gccgggaagt ccacgctgct
    25681 caagctcgtg gccggtgaat ccaacgcaga cggcgggcgg gtcagcctcg gtccgagcgt
    25741 ggtgctcggc tatttcgcac agcacagcat ggaaatcttg gatcccgaga agaccgtgct
    25801 cgagatcttg acccaggcct tcccccaatc ctccctcggt tcgatcaaga cgctgctcgg
    25861 cgcgttcggg ttctccgggg atgacgtcga caagcgttgc cgcgtattgt cgggaggcga
    25921 gaaggcgcgc gtggtgttgg cgcgcatgct gttcaatcca cccaatctgc tggtgctcga
    25981 cgagccgacc aaccacttgg acgtggacac caaagaaatg ctcgtcaaag ccttggccga
    26041 tttcgaaggc accttgctat tcgtgtcgca cgatcggcgt ttcttgtcgg ctctctccaa
    26101 tcgggtactc gaactcggta cggggaagcc gcacgcttac ggaggcggct acaccgaata
    26161 cgttcgcacg accgggcatg aagcccccgg cgtcagctga gcggagttcg agctcgggcg
    26221 acgcgactaa aggcccacca tcgaggtgac atagccctcg atggcggtgt gaacatcggg
    26281 aggaacggcg gtgaactcca gacccacggc ctcggttcca cgagcattcc ggacccattt
    26341 ggtgcgcgcg gcgagctcca ccagcttgcc agagccgggc agcgcgaacc gcactttgac
    26401 gagttcgtct gcgtcgcacg gcgcggccgt aaccaccagt aggcccgcga cggagatatc
    26461 ttcgctgcgc ccatcgatac ctttgccggt agggcgcaga attcgggtcg gcgttacgta
    26521 gggcgcgcgg ggaaagcgcc gacgcgctac cccgggaagc tcgggcggag ccgcggcggg
    26581 cagcttcggc gcgacgggcg cgggacgcgc tacggggcgt agcgcacgaa ggccgagcaa
    26641 agtggcgccc gtgtctggaa cggggaattg agtcagcgac tccacgaacg cgctcatgct
    26701 cgggtagcga tcgttcggat ctcgcgccag agccttctcg atcgtggcgg caagctccgc
    26761 gggaacagtg gggctcttgg cacgaatcgg cgggatctcc tgggtcgaag acttgagcag
    26821 cacttcgccg aagctgccat cgaacggcac attgccggtc aagcactcgt acagcgtcac
    26881 cccgacggca tagatgtcga cccgctcgtc catctggccg tgacccatca tctgctcggg
    26941 agccatgtat tccggagtgc cgagcacggc gccctgctgg gtcaacttgt tggagtcgct
    27001 ggtcgtgcgg gcaatcccga agtcgatcag cttgaccact tcacgtccgg tctcgtcgcg
    27061 cgcgacgaag atgttgctcg gcttgatgtc ccgatgcaga acgccacgac cgtgcgcgac
    27121 cgccagcgcc tcgcacagct gacgaccgac ctgcaccgtt tcgtgcacgc caattcgcaa
    27181 gcgcacggcc aagatgcctt gcagggtgcg accctccagc aactccatga ctagataggg
    27241 gccgaactcg ggcaactccc ctgcatccag cacctgggcg acgaacgggt ggcgcgcggt
    27301 cgcgagagca atcgcttcgc ggagcaaacg attgcgggtt tccgcgacgt gcgcgtaagt
    27361 ctccgacagt aacttcaccg caaccgggcg cttggtgaac aggtgttgtg cttcgtacac
    27421 cacgccagcc gcaccgcgag ccacctcacg cttcaagagg taacggcgat ctaggatcgt
    27481 tccaaccgtg tggcgcgctc gactcatgcg tgggtgcttg ctggaatgcg accgggttca
    27541 aacagccggg ccgcgcatcg gcagatcctt gaacggtcga ctccggcacg atatcacgcc
    27601 cccaaagcgc tcggcgtgcg cgaaaatcgc gcgggtcgac ggccggctga ctacgcgcgc
    27661 acccagctct cgaacactcg ttcgcgatag cggaaaggcg gcgttgagcg acaactccgg
    27721 cgtcacactt acccgaacgc ctgcgccgga aactgtagca ggcagggcag ctagggagtg
    27781 cccagccgaa ccttcatggg ttcatgctgc ggaggcagca cgccccatcc gcgtcgaact
    27841 cgaatgctca ccggcttctg actcaggagt cggccgtggt aagcaggttg actgccctcg
    27901 ccggcatctt cttcgcgggc ctcgaaacga gcgtgaacca agtacagccc gggagagcgc
    27961 atggcacctg ccgggcacag ctccacgagc cggctggtcg cggaaattct gccacctggc
    28021 cgtaggctct ggaaggcgcg ccgatctggc gcccggtcgt cgggaccagg ctcgcacgtc
    28081 accaatccac gtggcccgga gatctcgaag gacagcaatt cacgacggaa gaacacgcgc
    28141 tgggtactct tgctgcggtt cacgatcgaa acggccagag tagcgtcgcg ctcggtgcgc
    28201 gcatccgaac cgcgcgtgag ctggagagcc aagggctcgg agtccgcttc ggggggcgct
    28261 tcggcgtagt ctgggccgag ctcgatcgaa gcggacacga gttgcttcag gagcgagctt
    28321 tccgccgtgc ccggcgccgc ggagctggac tggtcattcg gggcggatgc ggggcccgcg
    28381 tcacgctccg tgctgccgct caggcgctcc gcaacgaagg gcggcgcttg cgccacgagc
    28441 accttttccg acttgcccgc cttccacacg gtcttggtct tctcgggcca cccgaggcgc
    28501 gcaacgaccc gagcccccgg cttgagtggc gacgtgcccg tccaagagag gcagtagagg
    28561 cgcggatcga agccatcgac cacggcttcg cccggttcga gctgcgttcg caagttcggg
    28621 tcttcgacgg acgggaacag gtcgcgaggc aacgtgcagg taaccggctt cggcggcttg
    28681 actggccgat gatacttcgc gttctgcggc ggctcgggcg ccttcggcgt gatctccaag
    28741 ctcaacgtgc gcaaatccgg attgactcgc accggctcgg tgccgcgatt ggcgatggcg
    28801 accagccacg gcgcgttcgg tccttgatcc acgatcgcaa gccgcagccc caggcgcgtt
    28861 cgttcgtcgg gagcacttgc ggttgcatcg agggcctgac gtacgcgccc gggttcatag
    28921 ctgggaggcg cgggcggttc agcggcgccg gcagggtcgc ccgctttttc gccagcatca
    28981 gctacggctg gtttacccgg agtcgacttc gactcaggta acgtgggccg agaaccagct
    29041 ctcgccgaac gaccgggctg gccacgctgg tccgaggatg cacccgcgac cgacgaaccg
    29101 ctcgccgctg aggcagattc ctcttggcac tcggctcgcc ccgactgcgt gccgacgcag
    29161 tccacgccga ttcgctggtt gcgcgccgca cctccacatc cgaccacctg gagcagagcc
    29221 aagcaacagg gaacgagtcc ggcgcgcgac acaatcatga ctcgactgtt tagttcgggc
    29281 cgcgcagcct gacaactgaa gcaagcagcc tgcagaggct cccctcaatc tatgcgccgg
    29341 ctgtcgacgc gataagcagc cccttcccga gccatccgcc cgttttgagg ctatgaagca
    29401 gaaacgcgcg ccgagcccca cacgtaagca gaccgtgcag accgcagatc ctccggaact
    29461 ctacacgttc gcgatttcgc acttctcgga aaaggtgcgc tgggcgctcg atatcgagcg
    29521 tgtgccttac cgggaacagc gcctcattcc aggggttcat ggctttacca cccgtggttt
    29581 tgccgccaaa agttcggtcc ccgtactgcg ccagggcaat cgcgtgatcc aaggcagtcg
    29641 cgagatcttg gacgcgctgc ccgtcctctt ctcggccacc acgctgcagc ccgcgacctt
    29701 gagccccgag caacgagcag aaatcgtcga atgggaggcc ctgttcgact acgccttcgg
    29761 gctcggagtg caaagactgg cgttcgacga gctgctgggg ctgccccgtg aggcggtcga
    29821 tttcatcgct caggacgcac cgggctgggc caagctgctc tacagggtgg gttacccgtt
    29881 cgtgcggcgt tcggtccgag atctatacga gatcaagcct cgaacggtgg accgcgcgcg
    29941 acgggcattt gcggacgcca tcgcgcgcac tgacaaacgg ctcgagaatt gcccctttct
    30001 cgtggcggag cagttgactc gggcggacat cacgcttgcg gccctgctcg cgccgctctg
    30061 ccgtccgcca caacacctgg ctcgatggcc caagaccgtc cctgggctgc aggaattcgt
    30121 aacgcccttc gagtcgggtg tgacttggaa atgggtcctt cgcatgtact cggagaggcg
    30181 ccacggctct ggctcggcca tagagcagca ggctgactcg gcgcgacgtc gtgagtgagg
    30241 tcaaaggcgc gcccggatag cgcgggcgcg gagcggacgg gcgcgctgag gtctgctcgg
    30301 tcttccaaat tccctcaccc ttttgccgcg aaaccgagtc taagctgcgg gcatgtccct
    30361 ggaacgcatt cgtgcgctcg tggagcgctt tcgccgggtg atcgcagacg gaggcgccag
    30421 cggtcccgcg gtgaatggct ggcaattcga caccgcaatc gggctcgaac tgctcgaatc
    30481 gatgctgttg gcgcggcatc tcgacgtcgc cgcgcatgag cttcgagcga gtggacacgg
    30541 ccactacacg atttgttcct cgggtcacga agccaatgcc gtgctcggtc ggctcaccac
    30601 accagatgac tcggccctgc tgcactacag aagtgcggcg ctctgcctag aacgggctcg
    30661 acaggtgcca gaagtggatg cggtgagaga tattgcgctt tcactagtgg ccgcccgcga
    30721 cgagcctgcc agcggtgggc gccataaggt gtttgggaac cgccgactgg ggatcatccc
    30781 gcaaaccagc accattgcct ctcaccttcc ccgcgcggtg ggcttggctt tcgcgctcga
    30841 acgcaaagct aggctcggtc ttctgcctgc gatcggctca ctcgcagagc ccggctcaca
    30901 tacgcggcgg agcccgatcg taatttgttc gttcggtgac gcg
//
LOCUS       DASTKU010000696         8245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1695528, whole genome shotgun sequence.
ACCESSION   DASTKU010000696 DASTKU010000000
VERSION     DASTKU010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695528"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VFQ61_24375"
     CDS             complement(<1..926)
                     /locus_tag="VFQ61_24375"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HET9957665.1"
                     /translation="MNPSKLSVYDEDFLLENDVARELYHDHAKHQPIIDYHCHLPPEH
                     VAKNHRFRTITEIWLDGDHYKWRALRTNGIPEVLVTGPAGDWDRFSAWARTVPKTLKN
                     PLYHFTHLELKKPFGIRDRLLSADTARGIYDDTNFRLADPEYSALGLMRQFNVAAVCT
                     TDDPADDLVHHRAYRESPAWDDVRMFPSFRPDGALEIEDPSALRNYVTRLGEVSGVDI
                     SSFNALLEALDRRHAAFHELGCRLSDHGLETMFSDDFSESEVEQIFEQALRGRVVDPG
                     QAQRYRSALLYELARLDSARGWTQQFHLGAIRG"
     gene            complement(1031..2206)
                     /locus_tag="VFQ61_24380"
     CDS             complement(1031..2206)
                     /locus_tag="VFQ61_24380"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET9957666.1"
                     /translation="MFSCRTSHDRTPSPFTQALEVARRAGTPLLDLTLSNPTESAIAY
                     EQTSILRALASPASMRYEPEPFGPRAARELLVQHWAERGFRVLEQQILISASTSESYS
                     LIFKLLCDPGHEVLVPVPSYPLFEQLARFEGVRAVPYELGFDGAWFIDFAELERRVTP
                     ASRAIVVVSPNNPTGHYLRRGELARLASFGLPIVSDEVFEAYPIDPPADRALSILEVE
                     TVPVFALSGLSKLAALPQMKLAWMTLGGPGAFIDEATRRLEHLADAFLSPSAPVLAAL
                     GSLLETRHTAQKQIQARLVENASWLRGAVAGSAVSVLPIEGGWYAMIRLPRIQDDESW
                     ALEVLERAQVVVQPGYFYDACDEGLLVLGLLTPPETFRAGVSRILRVVQDHLQIPNP"
     gene            2327..3325
                     /locus_tag="VFQ61_24385"
     CDS             2327..3325
                     /locus_tag="VFQ61_24385"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET9957667.1"
                     /translation="MTSLPTEWPNFDGMPDDETLTSTRPRGQKLSPYIWWDGEVRESD
                     APTVHYYANALHYGVAVFEGIRCYPTVNGPVLVRLRDHLERMLLSARLYGMQIPFDLD
                     ALTRAAVEVTRRNGIQNGYLRPLAYFGAGPIDLKPKRECPVHVFVAVRELGTFLGPDA
                     LRNGIRLTVSSWRRPHHTMLPTMAKASGHYANSVLAAHEAMDRGYDDAILLNQDGTVA
                     CATGHNVFFVRGGVLHTNDESSSIIPGITRDCVLSIARERNVPVQIRPFTREELLRAD
                     EVFLTGTAAEVTPVRELESCAYQTGKGTLGALFQKAYLRAVTGRDPKRLDWLTRVQ"
     gene            3777..5756
                     /locus_tag="VFQ61_24390"
     CDS             3777..5756
                     /locus_tag="VFQ61_24390"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9957668.1"
                     /translation="MSLIKRSQVTSTNGSKNGANGHANGNGYSNGHAQLSSATPDSGR
                     VSREAEAERKKARTLAKQQQVAEKIASSTTELSGAVSEATSAIGQLRQAVQDIAQGAT
                     QASGAAQESLTAMKAIAANVLRQKSLAETSGQKTDAIRTVIGNVTTDINKLVGNVEVA
                     ATRQFEATQLANELEQQAEKIGDIVKAVMRIADQTNLLALNAAIEAARAGQHGKGFAV
                     VADEVRTLAETSEKSAREIQTLVAQIKEAVRSVSQGIEASSLSAKQEAQKGYAIMSHL
                     QQIAGDMDTLLAGAQEIATGAVQMDRAAREGQSGSELISSAAEGQSSACDESQKTVDQ
                     QMSALEQAEQGTQELAALSEELRSSTDISKSAEEVASNAEQLSGAVEEVSRAAKQIFT
                     ALEQISKGAQQQAAASAQSLKAALEIEKGAQVADGRATEALDKARKMQDALSSNKASV
                     TEVSDGIQRSVEAGRKSQEQVTSLELVTRRIDKIVESISTVSIQTGMLAVNGAIEAAR
                     AGEYGKGFVVVATDIRNLANDSAANAEHIKDLVKAIQDQVHRVRKDLEETTSAAVSEA
                     EKARNIGTLLDSVAKDMSVVVQGNDGIRSAALEILTATVQAKTGVSQIATAADLAEKK
                     AAQAATASRQQEQAAQELAVRIEEISTLADGLQSN"
     gene            5767..7377
                     /locus_tag="VFQ61_24395"
     CDS             5767..7377
                     /locus_tag="VFQ61_24395"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9957669.1"
                     /translation="MQTPIQSAANGDSAPKQAAQKAASGELGTQQFVTFLVNDELFGV
                     PMGSVREIIRMPALVQVPLSPPSLDGLANLRGKVLPIIDLRSSFKFSRAEHSDSTRVI
                     VADCGALVGFVVDRVSSVISIDWDRIEPAATIQGTVDTRMMTGVIKPRGASNEPMVVL
                     LDLQKVLGEDFSSLQELGASTQANSSLGTSTSREPQDAAAAELQLVSFQVAGQEYAFP
                     INRVQEIVQVPSGFCEIPNTQSHVLGVMTLRDRLLPIVSLRELFHLPRTALEEQNRIV
                     VLTLDEAGTTSVGIVMDRVKEVLRLPASCVGQLPGLLTRESQTQEVDAICQLDSGKRL
                     VSVLSVEKMFRHQVISEALGSNSNTVEEEQEQVEESRSRESDDEAQMVVFRLGAEEYG
                     VAIDSVQEILRVADQFTHVPHAAEFIEGLISLRGAVLPVVDLRRRFNMPDRERDERQR
                     IMVFSVGGVRTGVIVDAVLEVLKVPSSSCEAAPRLSARQAEVIKRVANLERQKRMVLV
                     LEAERLFDSAELGQLRRVDGPGWEHSSC"
     gene            7356..>8245
                     /locus_tag="VFQ61_24400"
     CDS             7356..>8245
                     /locus_tag="VFQ61_24400"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET9957670.1"
                     /translation="MGTLKLLIVDDSALMRRHLVQIFEEAGGFEIRTARDGLDCLRQT
                     QEFQPDVVTLDLNMPHMDGMTCLSHLMIESPRPVVVVSSLTERDALPTLEALALGAVD
                     YVPKPGGTISANIRDIHQVLVSKVRGAARSRVRRARNLAARLQQPAPTRGRSPATPIA
                     REPLPRAALPRAVACSESLRLERLVLIGVSTGGPRALEELLPKLPADYPIPILVSVHM
                     PGAFTTQFAKRMGEACALQVVEVRRQVSVAAGTIYLAAGDTDMLVTRRSDKLVAIPTP
                     ADPSFLWHPSIERMVASTLQ"
BASE COUNT         1661 a         2539 c         2641 g         1404 t
ORIGIN      
        1 ccgcgaatcg caccgaggtg gaactgctga gtccagccgc gcgccgagtc gagccgcgcg
       61 agctcgtaca gcagcgcgga gcggtagcgt tgcgcttgcc ccggatcgac cacgcgcccc
      121 cgcaaagctt gctcgaaaat ctgctcgacc tccgattcgg agaagtcgtc gctgaacatc
      181 gtctcgagtc cgtgatccga gaggcggcag cccagctcgt gaaacgcggc gtgccgacga
      241 tccagggctt ccaatagggc gttgaagctc gagatatcga cgccgctgac ttcaccgagc
      301 cgtgtgacgt aattgcgcag cgcgctcgga tcttcgattt cgagcgcgcc gtcggggcgg
      361 aaactcggga acatgcgcac gtcgtcccaa gccgggctct cgcgataggc gcggtggtgc
      421 actagatcgt cagcgggatc atcggtggtg cacaccgcgg cgacattgaa ctggcgcatc
      481 aaaccgagtg cgctgtactc gggatcggcg agacggaagt tcgtatcgtc gtagatgcca
      541 cgcgcggtgt ccgcggagag tagacgatcc cgaattccaa agggcttctt cagctctaga
      601 tgggtgaagt gatagagcgg gttcttcagc gtcttgggca ccgtgcgggc ccaagccgag
      661 aaacggtccc agtccccggc gggaccggtg accagcacct ccgggatgcc gttggtccgc
      721 agtgctcgcc atttgtagtg atcgccgtcg agccaaatct ccgtgatggt gcggaagcga
      781 tggttcttcg ccacatgctc ggggggcagg tggcaatgat aatcgatgat tggctggtgc
      841 ttcgcgtggt cgtggtagag ctcacgcgcc acgtcgttct cgagcaagaa gtcttcgtcg
      901 taaacggata gcttggaagg attcacggct gactgggcgg gagcatagcg ccgacaagcc
      961 gctgcggtcc atgcggctcc gccgagacaa ttcatggctc cgccgcgctc gagcctctgg
     1021 aggcgtgctc tcagggattt gggatttgca ggtgatcctg caccacccgc aggatgcgcg
     1081 acactccggc gcggaaggtt tcgggcgggg tgagcagacc gagtaccagc aagccttcgt
     1141 cgcaagcgtc gtagaagtag ccaggttgca cgacgacctg cgcccgctcg agcacctcga
     1201 gggcccagct ttcgtcgtct tgtatacgcg gcagtcgaat catggcgtac caaccgcctt
     1261 cgatcggcag cacgcttacc gcgctgccag ccaccgctcc acgcaaccag ctggcgtttt
     1321 cgacgagacg agcttggatc tgcttttgcg ccgtatgccg cgtttcgagc aggctcccca
     1381 gagctgcaag cacgggcgcg ctcggggaaa gaaacgcatc tgccaagtgc tccaaacgcc
     1441 gggtcgcttc atcgatgaat gccccggggc cgccgagcgt catccaggcg agcttcattt
     1501 gcggcagggc cgcgagcttc gacaagccgg agagcgcaaa caccggtaca gtttcgactt
     1561 cgaggatcga cagcgcgcga tcggcgggcg gatcgatagg ataagcttcg aacacctcgt
     1621 cgctgacgat cggcagcccg aagctcgcca atcgcgcgag ctccccgcgg cgcagataat
     1681 ggccggtcgg attgtttgga ctcacgacca cgatagcgcg agaggcgggc gtgacgcgcc
     1741 gctcgagttc ggcgaagtcg atgaaccatg cgccgtcgaa accaagctcg tagggcaccg
     1801 cgcgcacgcc ctcgaagcgg gccagctgct cgaacaacgg atagctcggc accggcacca
     1861 aaacctcgtg ccccggatcg caaagcaact tgaaaatcag cgagtaggac tcgctcgtgc
     1921 tcgccgaaat gaggatctgc tgctccagca ctcgaaagcc gcgctccgcc cagtgttgga
     1981 ccaagagctc gcgtgcggcg cgcggaccga acggctcggg ttcgtagcgc atcgacgccg
     2041 gcgacgcgag tgcccgaagg atgctggttt gttcgtacgc gatggcggac tcggtaggat
     2101 tggagagggt caaatccagc aagggggtgc ccgcgcgccg cgccacctcg agagcctgag
     2161 tgaacgggct gggcgtgcgg tcgtgcgagg ttcgacagga aaacatgcga gtaagttggt
     2221 tctagacctg ggattcgcga ccgtagcgag gaaatcggcg ggttgcagga ggacgccgcg
     2281 tcggggggcg gactatactg gcgtcctact ccggagaccc gctgaaatga cctcgcttcc
     2341 cacggaatgg ccgaacttcg atggcatgcc cgacgatgag acgctgacct cgacgcgccc
     2401 acggggccag aagctctcgc cgtacatttg gtgggatggg gaggtacgcg agtcggacgc
     2461 gcccacagtc cactattatg ccaatgcctt gcattatgga gtcgcggtct tcgaggggat
     2521 tcgctgctac ccgaccgtca acgggcccgt gctcgtcagg ttgcgcgatc acttggagcg
     2581 catgctgctc tcggcccgcc tctatggcat gcagatccca ttcgacttgg acgcgctcac
     2641 gcgcgcggcg gtggaggtta cacgccgcaa cggcattcag aatggatatt tgcgtccgct
     2701 cgcctacttc ggcgcgggcc ccatcgactt gaagccaaaa cgcgagtgcc cggtgcatgt
     2761 cttcgtagcc gtgcgcgaac tcggaacgtt cctgggcccg gacgcgctcc gcaacggcat
     2821 ccggctgacg gtctcgagct ggcgtcgccc gcatcacacc atgctgccta cgatggctaa
     2881 ggctagcggc cactacgcca acagcgtgct cgcggcccat gaagccatgg accgcggcta
     2941 cgacgacgcg atcctgctca atcaggacgg cacggtcgcc tgcgccacgg gtcacaacgt
     3001 gttcttcgtg cgcggcggcg tgctccacac caatgacgag agctcctcga ttataccggg
     3061 tatcactcgt gattgtgtgc tgagtatcgc ccgcgagcgt aatgtccccg ttcagattcg
     3121 accgttcacc cgtgaagagc tgttgcgtgc ggacgaggtg ttcttgaccg gcacggccgc
     3181 ggaagtcacg cccgtgcgcg agctcgagag ttgcgcctat caaaccggca agggcacgct
     3241 cggcgcgctg tttcaaaagg cctatctgcg cgccgtaact ggtcgcgatc cgaagcgttt
     3301 ggactggctt acgcgcgtgc aatgagtgga gtgcgtcgcc gcggccgtag tcgagtcagg
     3361 gccacgctcg atgggcacac cttcattggc ggagctctgc tcatccatcg atgacgtgct
     3421 ttgcgtcacg tccgcgtcac gacaaccgtg cgcggcccga cgcaaatttt tccgagcggg
     3481 agactacggc catttcggtt gtagaagcac cgattcgcca cagcgctgag tggcggtatt
     3541 ccgtcacgcg tacgtatgtc taagcagctc ttaaatcgca tttcccggac gccgtcatag
     3601 tccccaatca tctcgtatct agttcgctgg ctcgcaacgg tatcgcgcgc ccgaacgtgg
     3661 atcgaggtgg cgcaacgtag ggaacatcgg cagcgcggag gcgcgcttcg ctagcagtgt
     3721 tcgctgacga cgcggtccag acaacaacag caatcgatac aggggaagca gcgagcatga
     3781 gcctcatcaa gcgcagtcaa gtcacctcca cgaacggctc caagaacggc gccaacgggc
     3841 acgccaacgg caatggctat tcgaatggcc acgcccaatt gagcagcgcc actcccgaca
     3901 gcgggcgcgt ctcccgcgaa gcggaggccg agcgcaagaa ggcccgaacg ctcgcgaaac
     3961 agcagcaagt cgcggagaag attgcgtctt ccacgaccga gctctcgggt gcggtatccg
     4021 aggcgacgag cgccatcgga caactccgcc aagccgtcca ggacatcgcg caaggtgcaa
     4081 cccaggcgtc gggggccgca caagagagcc ttacggcgat gaaagccatc gccgccaacg
     4141 tgctgcggca gaagtcgttg gccgaaacct cgggccagaa gacggatgcc attcgaaccg
     4201 tgattggcaa cgtaacgacc gacatcaaca agctggtagg aaatgtcgag gtcgccgcga
     4261 cccgccaatt cgaagccacc cagctcgcca acgagctgga gcaacaagcc gaaaagatcg
     4321 gagacatcgt caaggcggtg atgcggatcg cggaccaaac caacttgctg gccctgaacg
     4381 ccgcgatcga ggccgcccgc gcgggacagc acggaaaagg ctttgccgtg gtcgccgatg
     4441 aagtgcgcac actggccgaa acttcggaga agtcggcacg tgaaatacag accttggtcg
     4501 cgcagatcaa ggaggccgta cgctcggtat cgcaaggcat cgaggccagc tcgctgtcag
     4561 ccaagcagga agcccagaag ggctacgcga tcatgagcca tctgcagcag atcgccggag
     4621 atatggatac cttgctggcg ggcgcgcaag agatcgccac cggcgcggtg cagatggacc
     4681 gcgccgcccg tgaagggcaa tcaggctccg agctcatctc ctcggccgca gagggacagt
     4741 ccagcgcttg tgatgaatcg caaaagacgg tggaccagca gatgtccgcc ctggagcagg
     4801 ccgaacaagg cacgcaggag ctcgccgcgc tgtccgagga gctgcgctcg tccaccgaca
     4861 tctccaagag cgcagaagaa gtcgcgtcca acgcggagca actttccgga gccgtcgaag
     4921 aggtatcgcg cgccgcgaag cagatcttta cggctctgga gcagatctcc aagggagctc
     4981 agcagcaggc ggccgcatcc gcccagtctt tgaaggccgc gctggaaatc gagaagggtg
     5041 cgcaggtggc cgatggtcgc gccacggaag cgctcgacaa agcgcgcaag atgcaagacg
     5101 ccctgagctc gaacaaggct tccgtcaccg aggtgagcga cggcattcaa cgctctgtcg
     5161 aggcgggccg caagagtcag gagcaggtca cctcgctcga gctcgtgacg cggcgaatcg
     5221 ataagatcgt cgagagtatc agcaccgtca gcattcagac gggcatgttg gcggtcaacg
     5281 gcgccatcga agccgctcgc gccggtgagt atggaaaagg tttcgtggtc gtggccaccg
     5341 atattcgcaa tctggccaat gactccgcgg cgaacgccga gcacatcaaa gatctcgtca
     5401 aagccatcca ggatcaagtg caccgggtgc gcaaggatct cgaggagacc accagcgccg
     5461 ccgtcagcga ggccgagaag gcccgcaaca tcggcacgct gctggacagc gtcgccaaag
     5521 acatgagcgt ggtcgtgcaa ggcaacgacg ggattcgatc cgcggcactc gaaattctga
     5581 ccgccaccgt acaggccaag accggcgtca gccaaatcgc gaccgctgcg gatctagccg
     5641 aaaagaaggc ggcccaagcg gccaccgcga gccgccaaca agagcaggcc gctcaagagc
     5701 tcgcggtccg catcgaagag atatcgactc tagccgacgg cctgcaaagc aactgaggtg
     5761 cttccgatgc agaccccgat tcagagcgcc gccaacggcg actcggcccc aaaacaagcc
     5821 gctcagaagg cggcttcggg cgagctcgga acccaacaat tcgtcacctt cctggtcaat
     5881 gacgagctgt tcggggttcc catgggctcc gtccgagaaa tcattcgcat gcccgcgcta
     5941 gtgcaggttc cgctcagccc tcctagcctg gatggtttgg ccaacctgcg cggcaaagtg
     6001 ctgccgatca tcgatttgcg gagtagcttc aagttcagcc gcgcggaaca tagcgactcc
     6061 acgcgggtga tcgtcgccga ttgcggagcc ctggttggct tcgtcgtcga ccgcgtatcg
     6121 agcgtcatct cgatcgattg ggaccgcatc gagcccgccg cgacgatcca aggcaccgtg
     6181 gatacgcgga tgatgactgg agtcataaag ccccgcggcg cctcgaacga gccgatggtg
     6241 gtcttgctcg atctgcagaa ggtgcttggc gaggatttca gcagtttgca agagctgggc
     6301 gcgagcacgc aagcgaactc gagcctcggc acctcgacga gccgcgagcc acaggacgcg
     6361 gccgccgcgg agctgcagct cgtcagcttt caagtcgccg ggcaagagta tgcatttccg
     6421 atcaaccggg tgcaggaaat cgtgcaggtg ccgagtgggt tctgcgaaat acccaatacc
     6481 caatctcatg tactcggcgt catgaccctg cgcgatcggc tgctgccgat cgtgagtcta
     6541 cgagagctgt ttcacctccc gcggactgcg ctcgaggaac agaatcggat cgtggtcctc
     6601 accctcgacg aagccgggac cacctcggtc ggcatcgtga tggaccgcgt caaagaggtg
     6661 ctgcgtctac ccgcttcctg tgtaggtcag ttgccgggtc tgctgacgcg cgagtcccaa
     6721 acacaagagg tggatgccat ttgccagctg gactcgggga agcgcctggt gagcgtgctg
     6781 tccgtagaga aaatgtttcg acaccaggtc atttccgagg cgctgggctc gaactcgaac
     6841 accgtcgaag aggagcaaga gcaagtggag gagagccgca gccgagaaag cgacgatgag
     6901 gcgcagatgg tggtgttccg cctcggcgcc gaagagtacg gcgtcgccat cgattcggtg
     6961 caagagatcc tgcgcgtggc ggaccagttc acgcacgtgc cgcacgcggc cgagttcatc
     7021 gaaggcctaa tcagcctgcg gggcgcggtc ttaccggtgg tggacctccg ccgccgcttc
     7081 aacatgcccg atcgagaacg ggacgagcga cagcgcatca tggtcttctc cgtgggcgga
     7141 gttcgaaccg gggtgatcgt cgacgcggtg ctcgaggtgt tgaaggtgcc gagtagctcc
     7201 tgcgaggccg ccccgcgcct ctccgctcgt caagccgagg tgatcaagcg ggtcgccaac
     7261 ttggaacgac aaaagcgtat ggtgctggtg ctcgaggcag aacgcttgtt cgattcagcc
     7321 gaactgggtc agctccgtcg tgtggatggg ccaggatggg aacactcaag ctgctgattg
     7381 tcgatgactc cgcgttaatg cggcgtcacc tcgtccagat cttcgaagaa gcgggcggtt
     7441 tcgagattcg aaccgcccgc gatgggctcg attgcttgcg tcaaacccag gagtttcaac
     7501 ccgacgtggt gacgctggat ttgaacatgc ctcacatgga cgggatgacc tgcttgagtc
     7561 acctgatgat cgagtcgccg cgaccggtgg tggtggtgtc gtcactcacc gagcgggacg
     7621 cactgcccac gctcgaggcg ctcgcgctcg gtgccgtgga ctacgttccc aaaccaggtg
     7681 gcaccatctc cgcgaatatt agggatatcc accaggtgct ggtgagtaaa gtgcgtggag
     7741 ccgcccgctc tcgggtgcgg cgagcgagaa acctcgcggc gcgcctgcag cagccggcgc
     7801 caacacgcgg tcgatcaccg gccacgccga tcgcgcggga gccgttacct cgagccgcgt
     7861 tacctcgagc cgtggcgtgc tccgagagct tgcgactcga gcgtctggtg ctgatcggtg
     7921 tttccacggg gggtccccgc gctctcgaag aactgctccc aaaactgccc gcagattatc
     7981 cgattccgat cctggtttcc gttcacatgc ccggcgcatt cacgactcaa tttgccaagc
     8041 gcatgggcga agcctgtgcg ctgcaggtcg tggaggtgcg ccgacaagtg tcggttgccg
     8101 ccggcaccat ctatttagcg gccggagaca cggatatgct ggtgactcgg cgctccgaca
     8161 aactagtcgc gatccccact ccggccgatc catcgtttct ttggcatccg agcatcgagc
     8221 gcatggtcgc cagcacgctg cagca
//
LOCUS       DASTKU010000697         5377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1473045, whole genome shotgun sequence.
ACCESSION   DASTKU010000697 DASTKU010000000
VERSION     DASTKU010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473045"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VFQ61_24405"
     CDS             <1..682
                     /locus_tag="VFQ61_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET9957671.1"
                     /translation="KTFRWVGRYASNLQSRTTLDTQLNVFADFKPRLPEAYRDSPFVL
                     LGNIQPQLQLDVLEQAQGRKFVVADTMNFWISSEPKALRKVLEKVDLLVINDEEVRQL
                     AEEHNVKKAARAVLGMGPKRLVVKRGEFGAMLFDESGMFFAPAYPLESEIDPTGAGDT
                     FAGALVGFLASQPEVNPGSLRRAVMTAATVASFCVEGVGTRRTRALNRDDVAVRLEEL
                     RQLVHFAP"
     gene            complement(803..1096)
                     /locus_tag="VFQ61_24410"
     CDS             complement(803..1096)
                     /locus_tag="VFQ61_24410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957672.1"
                     /translation="MATRSKTEMLNTLRTLIQQSLQELHRGAAHSRLTRVSGAVDGYM
                     RALLDAGIAKQDELLAIVASERALQDGPATAFVQPETASAEASLPSKSATLAA"
     gene            1358..1783
                     /gene="panD"
                     /locus_tag="VFQ61_24415"
     CDS             1358..1783
                     /gene="panD"
                     /locus_tag="VFQ61_24415"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HET9957673.1"
                     /translation="MKLNVFKSKIHRATVTHADLNYEGSVTIDEELMAAAEILPYEAV
                     HIWNVTRGTRLMTYALKGPAGSGCVCINGAAAHLNQPGDLVILATFAEMTPEEAREHM
                     PRVVRVDARNVAIDDDSPELPGPILDSLPLARSTPLSRA"
     gene            1942..3285
                     /locus_tag="VFQ61_24420"
     CDS             1942..3285
                     /locus_tag="VFQ61_24420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957674.1"
                     /translation="MSSVSVVSALSVHPQLAPMASLVRASAMDATLARRADYASRHRL
                     ASGPAVGPGHVPEGLTREVSDTPFGNPFDILERGVEREEERALLGALLAFGLKEEPYD
                     GDSLRAMGSHLVWLAAHTPCSAFPFIDAVLSEVQASAIWGGAALILTQPEAAPSDFGM
                     TEALVSASVLQASSSKEAYRLRLEAAEIESDPSVYALLTAGIDELGSVLRGELQPAPR
                     GPIVTTVLAFTGLLFIAHAFRLIARAVFAYRRPAVLRLGPQGLELSHRVEMMGRVLND
                     HATVVPLFSLARITRETRFARAGLYVGLLALMLGTYFGTDLFVSGVRVPSGSGPLLGL
                     AGLFIVAGLGVDFVLSSAGDALRGKSRLVIVPTRGRTLCVGALDPAAADALLDDLVEY
                     TRRAGLVEPTDVSAKAPPTLADSDKQRPSRAPMLNETSVKAQPTEPAAADETSLN"
     gene            complement(3298..4731)
                     /locus_tag="VFQ61_24425"
     CDS             complement(3298..4731)
                     /locus_tag="VFQ61_24425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957675.1"
                     /translation="MDSWTLWRGRSDTRRRTWSLWLLASALIHLPMTPAAALLGLLGW
                     ALTRPSADDIPALPPITEIPVEILESDPADAQPVGGPKSTEPGPGATGEGVAVPTPKP
                     VPKPKPKPKPKPAERDRAEDAQAGAGGAGNATPPGKHAPAAGGSTGGEKKPEGIADPI
                     AAAGGARKVADPNANVRVLVDTEKLRSHPMGPRIGALLSGIYQWRDFFGPSGINPVND
                     IDRILIAGPQLRRSDEVVAVLQHRVGQERIRAALDRLVQRDASGAWLDSKVPVAKARA
                     DRADRYFVLPNPRIVVVSPESTLESAKRLGAQVRLPALPGATVVNAYVNAPARAFRGL
                     PFRVPDTLQWVRARIDATADNGAVVEIEAEDASEDAATQDAVFLTRNVTAATQLDLGL
                     LGQLLGTRPQRFVERVEFEAKGRRVVGKIVLTAAQIAALYDFAQMFLVPPAPRTPAPA
                     ARGPAAPGDASPSVPNPSTSSPRDAAP"
     gene            4880..>5377
                     /locus_tag="VFQ61_24430"
     CDS             4880..>5377
                     /locus_tag="VFQ61_24430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957676.1"
                     /translation="MRVSRLLRLWIGPAVGSFTWLGCDREPPPAPAPNPTASTVQAGR
                     PELPPELSPKTSGNGNASAASSANAASNSAAQQPATPAPGPGSWSVAPSFPADYKGTW
                     FAVTALGAGVYVEPSFEAKKIGYLRSGSRVPVEAKPSSKKNCTGGWYALQSGGFICGN
                     LGTTDM"
BASE COUNT          875 a         1734 c         1791 g          977 t
ORIGIN      
        1 gaaaaccttt cgttgggttg ggcgctatgc ctccaacctg cagagccgca ccacgctcga
       61 cacccagttg aacgtgttcg cggacttcaa gccccggctg cccgaggcct accgcgacag
      121 cccgttcgtt ctgctgggca acatccagcc gcagttgcag ctggacgtgt tggagcaagc
      181 acaaggccga aagttcgtcg tggccgacac gatgaatttc tggatttcca gcgaacccaa
      241 ggcgctccgc aaagtgctcg agaaggtgga tctgctcgtc atcaacgacg aggaggttcg
      301 gcaactcgcc gaggagcaca acgtcaagaa ggccgcccgt gccgtgctgg gcatgggacc
      361 gaagcggttg gtggtgaagc gcggcgaatt cggcgcgatg ttgttcgacg agtcgggcat
      421 gttcttcgca cccgcctacc cgctcgagag tgaaatcgat ccaaccggcg ccggcgatac
      481 gttcgctggc gcgctcgtcg gcttcctcgc gtctcagcca gaggtcaacc cgggcagcct
      541 gcgacgggcc gtgatgacgg cggcgaccgt ggcttcgttc tgcgtcgaag gcgtgggtac
      601 acgccgaacc cgcgccctga atcgcgacga cgtcgcggtc cggttggagg agctgcgcca
      661 gctcgttcac ttcgctccct gaccgtcggc tgacccgcgc cgcccgggag ctccggttca
      721 cgacagcgct tgggcggacg aactgcgcgc aagctcgagc ggctggcgcg cagcatcggg
      781 atgcgggaaa cgtatcgggg cactaagcgg cgagggtcgc ggacttcgac ggtagcgacg
      841 cctccgccga ggcggtctcc ggttgtacga aggccgtggc ggggccatcc tgaagagccc
      901 gctcgctggc cacgatcgcg agcagctcgt cttgcttcgc gatccccgca tcgagtaggg
      961 cacgcatgta gccgtccacc gcgccgctga cacgcgtcaa gcgcgaatgc gcggcgccgc
     1021 ggtgaagttc ctgcagggat tgttgaatca gggtccgcag ggtgttcagc atttccgttt
     1081 tggatcgggt cgccattgcc aagtctccgg agctacgagc tcgtacgtcg acttcacgcc
     1141 tatcggaagt tgctgatctg ggccaagttc gtatcttcct tcggagcaac ccgtcggtgc
     1201 caaccagcac gctcgtgcaa gcaaggccgc cagcacccaa gggacaggtg taacggggct
     1261 ttgacggtga gcgcgcgcag acctatgctg tcgataagtc cgacaagtgg acccggaacc
     1321 gctcgggccg ctatgccgcg acgcattagg agccgttttg aagctcaacg tattcaagtc
     1381 gaagatccac cgtgccaccg tcacgcacgc ggatcttaac tacgagggta gcgtcaccat
     1441 cgacgaggag ctgatggctg ccgcggagat cctgccgtac gaggcggtac atatctggaa
     1501 tgtgacccgg ggcacgcgct tgatgacgta cgcgctgaaa ggccccgccg gctccggctg
     1561 tgtttgtatc aatggcgcgg ccgcacacct gaatcaaccg ggggatctcg tcattctggc
     1621 gaccttcgcc gagatgactc ccgaggaagc ccgggagcac atgccgcgcg tcgtgcgcgt
     1681 ggatgcgcga aacgtcgcga tcgacgacga cagtcccgag ttgccagggc cgatcttgga
     1741 ttctttacca ctcgcccgtt ccaccccgct gtctcgcgcc tagggtgtga gggtgctctg
     1801 gaggccaatc gggagctagc gtcgcggcgc gagccgaacg cgcggagcca gtattcgagg
     1861 gtgtgagacg ccgggttcgc cgctttcctc aggttttcgg acattccatg ataagcaggc
     1921 agcgccagta tgactgctga cgtgagtagc gtttcggtgg tgtccgcgct gtcggtacac
     1981 cctcagctcg ccccgatggc cagcctggtt cgagcttcgg ccatggacgc gacactcgcg
     2041 cggcgcgccg actatgcgtc acgccaccgc ttggcgagcg gtcctgccgt gggtcccggt
     2101 cacgttccgg aagggttgac gcgggaggtt tcggacacgc cgtttggcaa ccccttcgac
     2161 attttggagc gcggcgtgga gcgcgaagag gagcgagctc tgctcggagc gctactcgcg
     2221 ttcgggctga aggaggagcc atacgacggt gacagtctgc gcgcgatggg ctctcacctg
     2281 gtctggctcg cggctcacac gccttgcagc gcgtttccat tcatcgacgc ggtgctctcg
     2341 gaagtccaag ccagcgctat ttggggcggt gcggccttga ttctgacgca acccgaggcg
     2401 gccccctcgg acttcggaat gaccgaagcg ctggtgagcg cctcggtttt gcaggccagt
     2461 tcttccaagg aagcctatcg cttgcgcctg gaggcggccg agatcgagag cgatccttct
     2521 gtgtacgccc tgctgaccgc gggcatcgac gagctgggga gcgtcctgcg cggcgagcta
     2581 cagccggcgc cgcgcggtcc gatcgtgacc acggttctcg ccttcaccgg cctgctcttc
     2641 atcgcacacg ctttccggtt gatcgcgcgc gccgtgttcg cgtaccggcg cccggcggtg
     2701 ctgcgtctcg gcccgcaggg gctcgagctc tcgcatcgag tcgaaatgat ggggcgtgtt
     2761 ctgaatgacc acgcgacggt ggtgccgttg ttcagcttgg cccgcatcac gcgcgagact
     2821 cggttcgcac gggccggctt gtacgtgggc ctgctcgcgc tgatgctggg cacctacttt
     2881 ggcacggacc tgttcgtctc cggcgttcgg gtcccgagtg gttcgggtcc cctgctcggc
     2941 ctggccggtc tgttcatcgt ggcggggctg ggcgtcgatt tcgtgctctc cagtgcgggg
     3001 gatgcgctgc gcggcaagtc acggctcgtg attgtgccaa ccagaggccg aacgttgtgt
     3061 gtaggcgccc tcgatccggc cgccgccgat gcgctgctcg acgatttggt cgagtacacg
     3121 cggcgtgcgg gattggtgga accgacggat gtatccgcga aggctcctcc cacgctcgcg
     3181 gacagtgaca agcaacggcc gtcgcgcgcg ccgatgctca acgaaacgag cgtgaaagcg
     3241 caaccgaccg agcccgcggc ggctgacgag acatcgctga actgatctgg actcctctca
     3301 gggagcggcg tctcgcgggg agctcgtact cgggttcggg actgacggcg aagcgtctcc
     3361 cggcgctgct gggccccgtg ccgctggggc cggggtgcga ggcgcgggcg gaaccaggaa
     3421 catctgcgca aaatcgtaca gcgcagcaat ttgcgcggca gtcagtacaa tcttgcctac
     3481 cacgcggcgg cccttcgcct cgaactctac gcgctcgacg aagcgttgcg gacgcgtgcc
     3541 cagcagctgt cccaaaagac caaggtcgag ttgggtcgcg gcggtgacgt tccgcgtgag
     3601 aaacaccgcg tcttgagttg ccgcatcttc gctcgcgtcc tccgcttcga tttcgacgac
     3661 cgcgccgttg tcggcggtgg cgtcgattcg cgctctgacc cattgcaaag tatctggcac
     3721 tcggaagggc aaaccgcgga aggcgcgcgc gggcgcgttg acgtatgcat tcaccaccgt
     3781 cgctcctggt agcgccggca agcgcacctg cgcgcccaaa cgttttgcgc tctcgagcgt
     3841 gctctcgggg ctgaccacga cgatccgcgg gttcggcaaa acgaagtagc gatccgcgcg
     3901 atccgcgcgc gctttcgcga ccgggacctt gctgtcgagc cacgctccgc tcgcgtcgcg
     3961 ttgcaccaag cggtcgagcg cggcgcgaat acgctcctgc ccgactcggt gttgcagcac
     4021 cgcgacgacc tcgtcggacc gccggagctg aggaccagcg atgagaatac ggtcgatatc
     4081 gttgaccggg ttgatgccgc tcgggccgaa gaaatcccgc cactggtaga tgccggaaag
     4141 taaggctccg attcgcggtc ccatcgggtg cgatcgcagc ttctccgtat ccacgagcac
     4201 ccggacgttg gcgttcggat cggcgacttt gcgtgcgccc cccgcagccg cgatcggatc
     4261 cgcaatgccc tcgggcttct tttcgccgcc ggtacttcca cccgcggccg gggcgtgctt
     4321 acccggcggg gttgcattgc ccgcgcctcc ggcgccggcc tgagcgtcct cggctcggtc
     4381 gcgctccgcg ggcttgggct tcggcttggg tttcggtttg ggcacgggct tgggggtcgg
     4441 aacagcaaca ccctcgcccg ttgctcccgg accgggctcg gtgctcttgg ggcctccgac
     4501 gggctgcgcg tcagcggggt cggattccaa aatctcgacg gggatctctg tgatcggggg
     4561 cagcgccgga atgtcgtccg cagaaggtcg cgtcaatgcc cagcccagca ggccgagcag
     4621 tgccgctgcg ggagtcatgg gcaagtgaat caacgccgaa gcaagcagcc agagcgacca
     4681 ggtcctgcgg cgcgtgtccg aacgaccacg ccagagcgtc cacgaatcca cgattgcacc
     4741 atagtacctg tcgctaagtc gcgcgaaccg ctggcgtttc tccagcgcgt gctctggcac
     4801 gatctttcca gagcgtggcg cttggccact ccaataagtg gcctgacccg ctcgcctgtg
     4861 gaagaagagg tccccacgaa tgcgcgtttc ccgtctcctt cgactgtgga taggccctgc
     4921 ggtcggcagc ttcacttggc tcggctgcga tcgcgagccc ccgcccgccc ctgccccgaa
     4981 cccgaccgcg agcacggtcc aggcggggcg cccggaattg ccacccgagc tttcgccgaa
     5041 aacctcaggc aacggcaacg catcagcagc atcgagcgcg aatgcggcgt caaactcggc
     5101 tgcccaacaa cccgcgactc cagcgccggg ccctggaagt tggtccgtcg ctccgtcttt
     5161 tccagcggac tacaagggca cgtggtttgc ggttacggcg ctaggcgcgg gcgtgtacgt
     5221 ggagccgagc ttcgaagcga agaagattgg ctatctacgc agcggtagcc gtgtgccggt
     5281 ggaggccaag ccgagctcca agaagaactg cacggggggc tggtacgctc tccagtctgg
     5341 cgggttcatt tgcggcaacc tcggaacgac cgacatg
//
LOCUS       DASTKU010000698         4919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1473046, whole genome shotgun sequence.
ACCESSION   DASTKU010000698 DASTKU010000000
VERSION     DASTKU010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473046"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="VFQ61_24435"
     CDS             complement(<1..1616)
                     /locus_tag="VFQ61_24435"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET9957677.1"
                     /translation="MLAEQDTPDGSRRVALSRRFLVAFVTPLCLLLAVGALLALQVAR
                     MSEAAAWLEHTDHVLMKLSELDRRLANQEAALRGYQLSQLQVFLDDHERAELRTISSS
                     LEQLLVDNPAQRARLKEFQQRHALWEEAAKRGATRGVQDVVSDMTQRKRLADDASATI
                     ATMRETELSLRAQRNDDLAAKTLVTKIAFIVLLLATAIAIGVLSRRSLLDITRRFNEL
                     LQRERTSNRRLETEEWLRSGLLELSEAIRGDLTLERLAQQATSALSRYMRADAGALFV
                     AEPAGLRRVAGYGIDHDTAGSAFFTHGEGIVGRAAQEPGVIHLKELPPNFLKVRGGTG
                     SAEPNHLLISAVRHEGETLGVIELGFFNEPKAQTLSLFERVNETLGSGLRTALQRLRM
                     KELLEESQRQAEELQTQQEELRVANEELHEQSDALQEAQQQLRDRQLELETKNADLRQ
                     QTVYLQEARRSLQDKNNELERTSRYKSEFLANMSHELRTPLNSSLILAKLLADNRENN
                     LSAEQVRYASTIYSAGNDLLTMINDILDLS"
     gene            1997..2464
                     /locus_tag="VFQ61_24440"
     CDS             1997..2464
                     /locus_tag="VFQ61_24440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9957678.1"
                     /translation="MTTKSNDSESDRSSDGHGPSELGPGKEPAERRQVRVLVVDDHED
                     SAELLLEFLAGRACEARAASDGTSALLLAQEFVPDVALLDIGLPDMDGYELGARLLQI
                     PELRGLRMVALTGYSGHAEQVRAREAGFHAHVEKPVDIAKLFSLLDRLSPSLA"
     gene            2682..4112
                     /locus_tag="VFQ61_24445"
     CDS             2682..4112
                     /locus_tag="VFQ61_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9957679.1"
                     /translation="MKDRILIVDDDQGQCDLLAMVLERHGYEPISTTSPRTALERVAK
                     EEFDAVITDLEMTEMGGLELCERMLGTSPNVPIVVVTGQGSMDAAIKAMRVGAYDFLT
                     KPVDSKVLGLSVARAVQHRKLNSEVKRLREAAGSPPPEGFVVGSSTAMKRLADLVSRV
                     SQSDASVLIHGETGTGKELVAKAIHGASPRKGPFVAINCAAVPANLLESELFGHARGA
                     FTDAKNPRQGLFVEATGGTLFLDEIGEMPLEMQAKLLRALQERRVRPLGSNVEVEFDA
                     RIVCATHRDLEAEVEKRRFREDLYYRVNVVKIDVPPLRERGADILQLASFFLRKFSAR
                     SSKGNLSLSPQAAERMLSYDWPGNVRELENAMERAVALARLDQIVVEDLPDKIRSYRA
                     DRFVVSADDPQEVVSLEEIERRYIYRVIKILGGNKARAAELLGLDRRTLYRRLEKYEG
                     KTNGKHASSRAPGAPDSGPTETGESE"
     gene            4109..4807
                     /locus_tag="VFQ61_24450"
     CDS             4109..4807
                     /locus_tag="VFQ61_24450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9957680.1"
                     /translation="MSDSPQDGASYLKGEFLERIAHELRGPAGVMLGVLDELEFAMTT
                     DPARVVELLRMARRSTRRLLRTAERLQHAAQLDSGEVQWQRDPHDLRELVRTAVENAE
                     RIEARKGIQVQLTLPEAPCLALVDAPWLNAAILELVSNAIRNARTSVRISLASNSESA
                     GFELIFADDGAGFAGPITPRFSPPPRRQGLGLSLALVEDVVRGHEGKLTFESPTEPEA
                     RGTRVHITIAGPGR"
BASE COUNT          922 a         1515 c         1549 g          933 t
ORIGIN      
        1 ttggacaaat ccaagatgtc gttgatcatc gtgagcaggt cgttgccagc cgagtaaatc
       61 gtgctcgcat accgcacctg ttccgcgctg aggttattct ctcggttgtc agccagcagc
      121 ttggccaaga tcagcgagct gttcaacggt gtccgcagct cgtgggacat gttggcgaga
      181 aactcggact tgtagcggct ggtccgttcg agctcgttgt tcttgtcttg gagcgagcgc
      241 cgcgcctctt ggagatacac ggtctgctgg cgtaaatccg cgttcttggt ttcgagttcg
      301 agctgacgat cgcgcagttg ctgctgggct tcctgcaacg catcactctg ttcgtggagc
      361 tcctcgttgg cgacccgtag ctcctcctgt tgggtctgca gctcctccgc ttgccgttgc
      421 gactcctcca acaactcctt catgcgcaag cgctgcaaag ccgttctcaa accggagccc
      481 agcgtctcgt tcacgcgctc gaacaacgag agcgtttgcg ccttcggctc gttgaagaag
      541 ccgagctcga ttaccccgag cgtctctccc tcgtgtcgaa cggccgagat caggaggtgg
      601 ttcggctcgg cggacccggt gccaccacga accttgagaa aattcggggg cagctccttg
      661 agatggatca cccccggctc ctgcgcggct cgtccgacga tgccttcacc gtgggtaaag
      721 aaggcgctgc ctgcggtgtc gtgatcgatg ccgtacccag ccacccggcg cagccctgcc
      781 ggctccgcca cgaacagggc gccagcgtca gcgcgcatgt atcgggaaag cgcgctggtc
      841 gcttgctgcg ccagtcgctc gagcgtcaga tcaccgcgga tcgcttctga cagctccaac
      901 aaaccgctcc gcaaccactc ctcggtctcc aaacgtcggt tcgaggttcg ctctcgctga
      961 agcaattcat tgaagcgccg cgtgatgtcg agcaggctgc gtcgcgatag aactccgatc
     1021 gcgatcgccg tcgccagtaa cagcacgatg aacgcgatct tcgtgaccaa cgtcttggcg
     1081 gccaagtcgt cattgcgctg agcgcgcaag ctgagctcgg tctcgcgcat ggtcgcgatc
     1141 gtggcgctcg catcatccgc caagcgcttg cgctgagtca tatccgaaac gacgtcctgc
     1201 acccctctcg tcgcgccccg cttggccgct tcttcccaga gggcgtgcct ttgctggaac
     1261 tccttgagtc gcgcacgctg ggctgggttg tccacgagca gctgctcgag cgaagaactg
     1321 atggttcgaa gctcggcccg ctcatgatca tcgagaaaga cctgcagctg actgagctgg
     1381 tagccgcgta gtgcagcctc ttgattggcg agccggcggt cgagctcgct caatttcatc
     1441 aggacatggt ccgtatgctc gagccaggca gcggcctcgc tcatgcgggc cacctgcaat
     1501 gcgagcaggg ctcccaccgc cagtaggaga cacagcggcg ttacgaaagc gaccaggaac
     1561 cgccgggaaa gagcgactct ccgggatccg tcgggcgtat cttgttcagc gagcatgctt
     1621 tagggacgtt ctctctgggg ttcgtaccgc agccgcgcca gcggcgggct ggagttgtct
     1681 ccatgcggcc tcaccggtcg gcactggtct aacagtcgac ccaacccgtt atccgcgtgc
     1741 ttcggttgtg gcagcatgcc gcgcaatgca ctcaccgtgc cgggtatccc gtggcaagtt
     1801 ggtgagcaca ccttcttggc gagcacggac ggggagggac ggccgccaca gtggcgaaaa
     1861 cgtggtaagc ggaccactcc ggctcgcttt cagcttacga tgagcctcgg agattccctg
     1921 actcggagcc cataagctcc caccgaaacc acggaagcgc gtgcccgcag gggcacgacg
     1981 acggcacggc gagcgcatga ctacgaagtc caacgactct gaatccgatc gctcttcgga
     2041 cgggcacggg ccgagcgaac tcgggccagg caaagagccc gctgaacgtc gacaggtgcg
     2101 cgtcctggta gtcgatgacc acgaagattc ggcagaactt ctgctggagt ttttggcagg
     2161 aagagcctgc gaagcgcgcg cggcgagcga cggcacctcc gcgctcctgc tggcccaaga
     2221 attcgtgccg gacgtcgcgt tgctcgatat cggcttgccg gatatggacg gctacgagct
     2281 tggcgcgcgc ctgcttcaga taccagagct gcgcgggctg cgcatggtgg ctttgacggg
     2341 ctactcgggc cacgccgaac aagtgcgagc gcgcgaagct ggctttcacg ctcacgtcga
     2401 gaagcccgtg gacatcgcaa agctcttcag cctgctcgat cgcctaagcc cttccctggc
     2461 gtaaccagtt tccaacttat tggggcgggc tcgcgagtac agggcttgtc acgaggcgcg
     2521 aatgcgctaa caagaacgcc ccactgtgtc accatcgaaa tcgcgggaat tgagctgttt
     2581 ccgggagaag acgcaatttt ccccgtggca cgaaatctgc tgatgcaacg cacaccggac
     2641 cagtgcccca tcactgaacg tcacggtcct cggagagtgg aatgaaagac agaattctca
     2701 tcgtcgacga tgaccaaggc cagtgtgacc tgttggccat ggtgttggag cggcacggat
     2761 acgagccgat ctccaccact tcccctcgca cggcattgga gcgagtggcg aaggaagagt
     2821 tcgacgctgt gatcaccgat ctagagatga cggagatggg tggtctcgag ctctgcgagc
     2881 gcatgctggg caccagcccc aatgttccaa tcgtcgtggt cactggccag ggcagcatgg
     2941 acgctgcgat caaggccatg cgcgttggag cgtatgattt tctgaccaag ccggtagaca
     3001 gcaaggtgct cgggctcagc gtcgcacgcg cggtgcagca ccgaaagctg aattcagagg
     3061 tcaagcggct gcgtgaagcg gccggcagtc caccgccaga aggtttcgtg gtgggctcca
     3121 gcacggccat gaagcgcttg gccgatctgg tatcacgcgt gtctcagagc gacgcctccg
     3181 ttctgattca cggcgagacc ggcaccggta aggagctggt ggccaaggcc attcatggcg
     3241 cgagcccccg caaagggcca ttcgtagcca tcaactgcgc ggcggtgcca gccaatctgc
     3301 tcgagagcga gctgttcggg catgcgcggg gcgcgttcac ggacgccaag aatccacgcc
     3361 aaggcctgtt cgtcgaggcc acgggcggca cgttgttcct ggacgagatt ggggaaatgc
     3421 cgctcgaaat gcaggccaag ctgctacggg cactgcagga gcgtcgcgtg cgtccgctcg
     3481 gttcgaacgt cgaggtcgag ttcgatgcgc gcatcgtgtg tgcgacacac cgcgatctgg
     3541 aagccgaggt ggagaagcgc cgcttccgag aagacttgta ttatcgagtc aacgtagtga
     3601 agatcgacgt tcctcccttg cgcgagcgcg gcgcggacat actgcagctc gcttcgttct
     3661 tcctccgcaa gttctcggcg cgcagcagca aaggcaatct ctcgctgtcc ccacaggccg
     3721 ccgagcggat gctctcctac gactggcccg gcaatgtgcg cgagctcgag aacgcgatgg
     3781 aacgcgcggt cgcgctcgca cggctcgatc aaatcgtagt cgaggacctg ccagacaaga
     3841 tccgctcgta tcgcgccgac cgtttcgtgg tgtcagccga tgatccgcag gaggtcgtct
     3901 cgctcgaaga gatcgagcgg cgctacatct atcgagtgat caagattttg ggaggcaaca
     3961 aggctcgtgc ggccgagttg ctaggcttgg atcgacgcac gctgtaccga aggctcgaga
     4021 agtacgaagg caaaaccaac ggcaaacatg cctcctctcg agcgcctgga gctcctgact
     4081 ccgggccgac ggaaaccgga gaatccgaat gagtgattct ccgcaagacg gggcctctta
     4141 tctcaaaggt gaattcttag agcgcatcgc ccacgagctg cgcgggccgg ccggcgtcat
     4201 gctgggcgtg ctcgatgaac tcgagttcgc gatgactacc gatccggcgc gcgtggtgga
     4261 gctcttgcgc atggctcgcc gcagcacccg tcgtttgctt cgcaccgctg agcgtttgca
     4321 gcacgcagcc cagctcgact cgggcgaagt gcagtggcaa cgagaccctc acgatttacg
     4381 ggagctggtg cgtacggccg tagagaacgc ggagcgcatc gaggcccgca agggaattca
     4441 ggttcagctc acgttacccg aggccccttg cctggcgctg gtggacgccc cctggttgaa
     4501 cgcggcgatt ctggagctcg tcagcaacgc tatccgcaac gcgcggacga gtgtgcgtat
     4561 ctcgctcgcg tcgaatagcg agtccgcggg tttcgagctg attttcgcgg atgacggggc
     4621 aggcttcgcg gggcccatca ctccgcgctt cagcccgcct ccacgccggc aagggcttgg
     4681 gctttctttg gcgctggtgg aagacgtggt gcgcggtcac gagggcaagt tgacgttcga
     4741 gagccccacc gagccggaag ctcgcggcac ccgcgttcac atcaccatcg ctgggcccgg
     4801 acggtagctt cgctggcaat caccaaacgt agaaggccgt agaacttcga accgggaggg
     4861 gtgtgcctcc cgctcccgat aacacgatag tgttgtagtt cgattccgtg gctgcggga
//
LOCUS       DASTKU010000699         7976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1025380, whole genome shotgun sequence.
ACCESSION   DASTKU010000699 DASTKU010000000
VERSION     DASTKU010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7976
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025380"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..1643
                     /locus_tag="VFQ61_24455"
     CDS             327..1643
                     /locus_tag="VFQ61_24455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9957681.1"
                     /translation="MRHSLHLLTTPSLAIALSLFGCSLFQNSDDDDDAAPAGGMIGTG
                     GGGILPQGGSTSSTSSGGRTSSGGSMSTDPGTGGKTDMNACESLPGLDEACATTGKQA
                     DFRTVNMLVVIDKSGSMTDTPEGFDRDKWSALQTALPTALGEVATQMNFGLLLYPFDP
                     AGIPLDDCAKLGNCCSLPQAPEAINVPILPGDEAVPQIKQALTDASPGGGTPTAAALH
                     AAYTYFTSGAGAKLEGDKFVLLATDGGPNCNTSLSCEAERCTTNLDRRPDGSSQCTKK
                     DGASCCDGNPDRCLDDANVTKEIEALSRANIPTFVVGIPGTEVYSEYLNAFAEAGGKA
                     DPNSTTAKYYEVKAKGGVSGLTGVFSTITTQLVHSCDIPLDEPPPDSNKVNVAVDCEV
                     MRADTDWELLQSEEPNIIRLKGSLCDRVQADGVKRVDVILGCQSIR"
     gene            1728..3017
                     /locus_tag="VFQ61_24460"
     CDS             1728..3017
                     /locus_tag="VFQ61_24460"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9957682.1"
                     /translation="MQRYGYLLLKHAALAAVLSLAAGSCSSEEDDDGGSGAASSGNKP
                     GIPTGGSTSANGGNSSGGGGAGNTSGGKGGSVSTSGGGKTSTGGSGSGNATNGGAVAA
                     CEGLPFDPDNDPSVEACEGYAFEAEVVPVDMFIMMDRSVSMNELVPGTQTRRWDALHD
                     AVEAFVNSDSIGSIGAGIGFFGISGAKDDTLDCSVERYAEPTVEIGPLSETGPDLVQA
                     MADTVPGGLTPTTPALQGAIQYAKGWAKGHPGRATVVVLVTDGYPTQCPDQPSISAIA
                     DAAREGYQGTPSIRSFVIGLAAGFNLNNIAQAGGTKSAYLVDEGDVTNTFTNALLNIT
                     NSKIACEYEMPTSSDVDMEVDPSKVQIIYKPASGGQEEVPRLDSVTQCAINPNGGWYY
                     DNPSDPRSIKVCPCTCSRFQAGEVNVRVGCEPKIGIR"
     gene            complement(3155..5533)
                     /locus_tag="VFQ61_24465"
     CDS             complement(3155..5533)
                     /locus_tag="VFQ61_24465"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET9957683.1"
                     /translation="MGTTLLGCSSAVLLWPGKAPPQSRQPPRVTALPSAAGPARVPQL
                     ELESFAPFAAEPRFQPVTDALELDQLARAAQLLAEVRAQQPATDSQSLALWNGRLWER
                     AEQPERALPECEKALSLPGELTDYAELCVARVHVTLGLGKPALQLLSARQYGAATEPK
                     RLLLLSRAAKLSGDVERALQSVGQAAPLLKDAKERAEAELLQAELLITRASASNTALP
                     GARETDVELALTLLRRAFPNLQAKQRQVASELEQRGLSLLSPEKRAVLSVPTLEQNVQ
                     ELNGLLEGREREEAERRAGALLQTLDESTRNSPLGCELQLIQAKALGALRRYGKAEDV
                     LRVAREKCKADPERGPKIWFLSAKYAGSDGKSTQATNFYGEVEKLYPSDRLADDARFH
                     AALTWLELGDVARFTEMVLSMPDAYPRGDMTREGLFRLALRRMEVGAWGEALHVLDRT
                     LQLTGAEEKRGFDVLGRERFFKARALLALGQREPALAEFLDLVKTQPLSYYMLQSYTW
                     LEREAPGQARAALQAAEEAARSAPFRIASPSELDRPGFHRMLELLRIGELDAALAELD
                     VLGMAKTPAGPDLLWGIARLYERAGFSKFSADLAKRHLGEVSSMWPVNDWEKAWQLAF
                     PRPYWDLVRKHCGEARVEPGLAYAIMREESAFDPEATSIADAYGLMQLIVPTAQKAAK
                     GTNLRVSATSLKSPRVNIALGCRELAHLTGLFAENPLLAIPAYNAGPGRPQRWLRDRP
                     AMDFDLWVELIPFQETRRYFKRVVSSRAAYTYLYERERSESALLLPQRLAAL"
     gene            5671..6231
                     /locus_tag="VFQ61_24470"
     CDS             5671..6231
                     /locus_tag="VFQ61_24470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET9957684.1"
                     /translation="MARTPSTMLALDTELHDFSLPDVVSGKLLRSRDWLGKPLVVAFI
                     CNHCPFVKHLRAQLAEFGDYCRDKGVGMLAISSNDAANYPEDSPENMAKEATEFGYHF
                     PYLYDESQAVARAFDAACTPDFYVFDAQGKLAYRGQFDDSRPGNGIPPTGRDLYAAVD
                     ALLRGARPATEQRPSIGCNIKWKTAG"
     gene            6213..7385
                     /locus_tag="VFQ61_24475"
     CDS             6213..7385
                     /locus_tag="VFQ61_24475"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET9957685.1"
                     /translation="MEDGRLTPPRAGRVGWLRWVVTRTRHAPASCRLAPNKPSTRILA
                     GLARAALCALGLLSLGRLPALGAEPRARPAPLVDLHVDLPYQHVFQGRDFASGSGQYR
                     ALELLRAGVVGVVLPLFVPARVSPSGPRLSDLEGSYQRVFDALTRTPPYALPGCLASR
                     QSVRTFLAFEGAAPLLERQDSLVEFVARGVRSIGLVHTRDNRLAASSQQPSELAGTPA
                     GHKGLTTEGVDLVERAVRLRVAVDVSHASDATVREVAELVTSARVPMLATHSNSRVVR
                     NHPRNLADAEALAIARSGEVVGVNLHSPFLVAGRRARIADVVRHVRYWVRVLGVEHVG
                     IGSDFEGDIRPPEGLESVADLPKLALALEQSGLSRAAVEQIFGLNALRILCPPSER"
     gene            7421..7975
                     /locus_tag="VFQ61_24480"
     CDS             7421..7975
                     /locus_tag="VFQ61_24480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957686.1"
                     /translation="MSTLEDRCLEAIPHWLSLLGDDARELAELLGVDGAPHVKGAAAL
                     GLTFLVRSLEFISEVEEQGFAEAAIVLRVAAERVPAEELVGEQNSAALQTLARLGQDA
                     ALVREFLGEEFELLLALLHNPAAFALHGKTYEQLLEEPEALADLAYRARQFADQYSAP
                     SFARDPKSLIKLRAYLRVKTSPGA"
BASE COUNT         1381 a         2545 c         2593 g         1457 t
ORIGIN      
        1 tcctccacca ctcagtgggt gtcccttcgg tgtggtctcg caccgaactg aacatggcga
       61 cacccgcacc gctgccattc aaacacgaga tcgtgaacgt ccctatctaa ccctgagccc
      121 cgtaacgctc gaagcccagc atcactcagt acccggcaca cgcgacgacc tgcctcgtgc
      181 tatgaggtat cctgcttgca tcgcccccaa gctccgcacc tgcagagccc gggttgcgcc
      241 atcgcgcatc gagcggctat cctcaccagg ttctcgcgaa gttgccgtcc acgcgcggga
      301 tgctcgggga aaacggagtc aagaatatgc gtcactcttt gcacctgttg accactcctt
      361 ctctcgccat tgcgctgtcc ctcttcggct gcagcctgtt ccaaaactcc gatgacgatg
      421 atgacgctgc gcccgcaggc ggaatgattg ggaccggtgg tggcgggatt ctgccgcaag
      481 gtggttcgac gtcttcgacg tcgagcggcg ggcgaacgag ctcgggcggc tccatgagca
      541 ccgacccggg cacgggtggc aagaccgaca tgaatgcttg cgagagcctg ccgggcttgg
      601 acgaagcctg cgccacgacg ggcaagcaag cggacttccg caccgtcaac atgctcgtcg
      661 tgatcgataa gtccggcagc atgacggata cgcccgaagg gttcgatcgg gataagtggt
      721 cagcgctcca gacggcgttg ccgaccgcgc tcggggaagt ggccacgcag atgaacttcg
      781 ggctgttgct ctatcccttc gatccggcgg gcattccgct ggatgattgc gcgaagctcg
      841 gcaattgctg ttcattgccg caggctccgg aggcgatcaa tgtgccgatc ttacccggtg
      901 acgaagcggt gccgcagatc aagcaggcgt tgacggatgc ttcgccgggt ggaggcacac
      961 cgaccgcggc cgcgctccac gccgcgtata cgtacttcac cagcggtgct ggagcgaagc
     1021 tcgaaggcga caagttcgtt ttgctcgcga ccgacggtgg accgaactgc aacacgagct
     1081 tgagctgcga ggccgagcgc tgcacgacga acttggatcg taggcccgat ggctcgagcc
     1141 agtgcaccaa gaaggatggc gcgagctgct gtgacggaaa ccccgatcgt tgcttggacg
     1201 acgcgaacgt caccaaggag atcgaggcgc tctcgcgggc caatatcccg acgttcgtgg
     1261 tcggcattcc gggaaccgag gtttattcgg agtacttgaa cgccttcgca gaggcgggtg
     1321 gcaaagccga tccgaacagc accacggcca agtactacga ggtcaaggcc aagggcggcg
     1381 tcagcgggct cacgggtgtc ttctccacga ttacgaccca gctcgtgcac tcgtgcgaca
     1441 tcccgctgga cgagccgccg ccggactcca acaaggtgaa cgtcgcggtc gactgcgaag
     1501 tgatgcgtgc ggacaccgat tgggagctgc ttcaatcaga ggagcccaac atcattcgcc
     1561 tcaagggctc gctttgtgac cgagttcaag ccgacggcgt caagcgcgtc gacgtgatcc
     1621 tcggctgcca gtcgattcgc tgaccgctct cggatcggtc tcgtttttct ttcatcaacc
     1681 tgtcgcgtct tattccaacc cgccgcggcg acataaaggc aaacaccatg cagcgttatg
     1741 ggtaccttct tttgaaacac gcagccttgg ctgccgtcct ttctcttgcg gcgggtagct
     1801 gcagcagcga agaagacgac gatggagggt cgggagccgc gagctcgggc aacaaaccgg
     1861 gcatccctac cggcggctcc acctcggcga atggcggcaa ttcctccggg ggcggtggcg
     1921 cgggcaacac ctctggagga aagggcggca gcgtttccac cagcggtggc ggcaagacgt
     1981 cgacgggagg atcgggcagc gggaacgcga cgaacggcgg agccgttgca gcgtgtgaag
     2041 gtctgccgtt cgatcccgac aacgatccct cggtcgaagc ttgcgaaggc tatgcgttcg
     2101 aagccgaagt cgtgcccgtc gacatgttca tcatgatgga tcgctccgtg tcgatgaacg
     2161 agctggtgcc gggcacccag acgcggcgct gggacgcgct ccacgatgcg gtcgaggcct
     2221 tcgtcaactc cgactcgatc ggctcgatcg gggccggcat tggtttcttc gggatctccg
     2281 gcgcaaagga cgacacgctc gactgcagcg tcgaacgtta cgccgagcca accgtcgaga
     2341 tcgggccgct gagtgagacg ggcccggatt tggtccaggc catggcggac acggtgccgg
     2401 gtggtctcac gccgaccacc cctgcgttgc aaggcgccat tcagtacgcc aagggctggg
     2461 ccaagggtca tcccgggcgg gcgacggtgg tggtgctcgt gacggatggg taccccacac
     2521 aatgcccgga tcaaccgagc atctctgcca ttgctgatgc ggctcgcgag ggataccaag
     2581 gtacgccgtc catccgctcg ttcgtgattg ggctcgcagc gggcttcaac ctgaacaaca
     2641 tcgcgcaggc cggcggcacc aagtctgctt acttggtgga cgaaggggac gtgaccaaca
     2701 cgttcaccaa tgcgctgctg aacatcacga actccaagat cgcttgtgag tacgaaatgc
     2761 cgacctcgag cgatgtggac atggaggtcg atccgagcaa agttcagatc atctacaagc
     2821 cggcctcggg cggccaagaa gaagtgccgc gtctcgacag cgtgactcag tgtgcgatca
     2881 atccgaacgg cggctggtac tacgataacc cctccgatcc gcgcagcatc aaggtgtgcc
     2941 cgtgcacgtg ttctcgcttc caagcgggag aagtgaatgt gcgcgtcgga tgcgagccga
     3001 agatcggcat ccggtgaggc gcggcgaggc gctcgggtcg ttcgagtcgc gacgctagcc
     3061 agacctggct gacgagcaac tcgttagctc gaagaacaaa caagccgctc gctccatcgg
     3121 gagccagcgg cttgcgtctt tcgttgctcg ttccttacag cgcggcgagc cgttgtggaa
     3181 gcaacagcgc ggactcactg cgctcgcgct cgtagagata ggtgtaggcg gcgcggctgg
     3241 agaccacgcg cttgaagtag cggcgcgttt cttggaacgg aatcagctcc acccacaggt
     3301 cgaagtccat ggccgggcga tctctgagcc agcgttgagg acgaccgggg cccgcattgt
     3361 aggcaggaat agcgagcaac ggattttcgg cgaacagccc ggtcaagtga gcgagctctc
     3421 gacacccgag ggcgatattc acccgcggcg acttcaagct cgtcgcggac acccgcaagt
     3481 tcgtgccctt ggccgctttc tgcgcagtgg gcacgatcag ctgcatcaag ccgtaagcgt
     3541 ccgcgatgct ggtcgcctcc ggatcgaacg cggactcttc gcgcatgatc gcgtaagcga
     3601 gcccgggttc gacgcgcgct tccccgcagt gtttacgcac caaatcccag tacgggcgcg
     3661 ggaacgctag ctgccacgcc ttctcccagt cgttgacggg ccacatcgac gacacctcac
     3721 ccaagtggcg cttggccaaa tcggcggaga acttcgagaa accagcgcgc tcgtacaagc
     3781 gcgcgatgcc ccagagcagg tctggcccgg cgggtgtctt ggccatgccg agcacgtcga
     3841 gctccgcgag cgccgcgtcg agctcgccga tgcggaggag ctccaacatg cgatgaaagc
     3901 cgggacggtc gagctcgctt gggctggcga tccggaaggg ggcgctgcgg gccgcttcct
     3961 ctgcggcttg gagtgccgcg cgggcttggc cgggcgcttc gcgctcgagc catgtgtagc
     4021 tctgcagcat gtagtacgag agtggctgcg tcttgacgag gtcgaggaac tctgcgagcg
     4081 cgggctcacg ttgcccgagc gccagcaagg cccgtgcttt gaagaagcgc tcgcggccaa
     4141 gcacgtcgaa gccgcgtttt tcctcggccc ccgtcaactg cagggtgcgg tcgagcacgt
     4201 gcagcgcttc gccccacgct ccaacctcca tacgccgcag cgccagccgg aacaggcctt
     4261 cgcgcgtcat gtcgccgcgc ggataggcat ccggcatgct cagcaccatc tcggtgaagc
     4321 gcgcgacgtc tcccaactcc agccacgtga gcgccgcgtg aaagcgagcg tcatccgcca
     4381 agcgatcact cggatatagc ttctccactt cgccatagaa attcgtggct tgggtcgact
     4441 tcccgtccga gccggcgtac ttggccgaca agaaccagat tttggggccg cgctctggat
     4501 ccgctttgca cttctcgcgc gcaactcgga gcacgtcttc tgccttgccg taacgccgta
     4561 gtgctccgag cgccttggct tgaattaact gcaactcgca accgagcggt gagttccgcg
     4621 tgctctcgtc cagcgtttgt agcagcgctc ctgcccggcg ttccgcctcc tcacgttcac
     4681 ggccttccaa cagtccgttc agctcttgaa cgttctgttc gagcgtgggg acgctcaaga
     4741 cggccctctt ttcgggagac agaagcgaga ggccgcgctg ctcgagctcg ctcgcgactt
     4801 ggcgctgctt cgcctgcagg ttcgggaagg cgcgccgcag caatgtgaga gccagctcga
     4861 cgtctgtttc ccgtgccccg ggcaaagcgg tattcgaagc cgacgcccga gtgatcagca
     4921 gctccgcctg gagcagctcc gcctccgctc gctccttggc atctttcagc agcggagcgg
     4981 cctggcccac gctctgtagt gctctttcta cgtctccgct cagctttgcg gcgcggctca
     5041 gtaggagcaa gcgcttcggt tcggttgcgg cgccatactg tcgggccgag aggagttgaa
     5101 gcgcaggctt gccgagcccc agcgtcacgt gaacccgcgc tacacagagc tctgcatagt
     5161 ccgtcaactc gccgggtaag ctcagcgcct tttcacactc gggcaacgct ctctctggtt
     5221 gttcggcgcg ctcccacaag cgcccgttcc acagcgccaa agattgcgag tcggttgccg
     5281 gttgctgcgc gcgcacttcc gcgagcaact gcgcggcccg ggccagctga tcgagctcca
     5341 atgcatcagt aacgggctga aagcgcggct ccgccgcgaa cggcgcgaag ctctccaact
     5401 cgagctgagg cacccgcgcc ggcccagccg cgctcggcag cgccgtgacg cgcggcggct
     5461 gtcgagactg cggtggcgcc ttgcccggcc aaagcaagac cgcggacgaa caaccgagga
     5521 gggtggtgcc cacggccaag gcaatcctcc gactagacaa gagcttcggc ggcaacgccg
     5581 gcaagcttag cccgaaatcc cccgccgtgt gccgcccttc aaggcggact cggccttttg
     5641 ctgcggggag tttgctatgc tccggccgtc atggcgcgca ccccctccac gatgttggct
     5701 ttggataccg aattgcacga tttcagcttg ccggatgtgg tcagcggcaa gttgctccgt
     5761 tcgcgtgact ggctgggtaa gccgctggtg gtcgcgttca tctgcaacca ttgcccgttc
     5821 gtgaagcacc tgcgcgcgca gttggcggag ttcggagatt attgccgcga caagggcgtc
     5881 ggtatgctgg caatcagctc gaacgacgcg gccaattacc ctgaggactc gccggaaaac
     5941 atggccaaag aggccacgga attcgggtat cacttcccct acttgtacga tgagtcgcag
     6001 gcggtcgcgc gggcgttcga tgcggcgtgc acgccggatt tttacgtttt cgacgcgcag
     6061 ggcaagctcg cctaccgcgg ccaattcgac gatagccgcc ccggcaacgg cattcctccg
     6121 acgggacgcg acttgtacgc ggcggtggac gcgctgctgc ggggagcccg cccggcaacc
     6181 gagcagcggc ccagcatcgg ctgcaacatc aagtggaaga cggcaggctg acgcctccgc
     6241 gagcaggtcg cgtcggttgg ctccgttggg tagtcacgcg gacgcggcat gcgccggctt
     6301 cgtgccggct ggctccgaac aagccgagta cgcgaatcct cgccgggctc gcgcgcgcgg
     6361 cgctctgcgc ccttggactg ctctcgttgg ggcgcttgcc cgcgctcggt gccgaaccac
     6421 gagcgcgtcc ggctccgctc gtggatctgc acgtcgattt gccgtaccag cacgtgtttc
     6481 aaggtcgcga tttcgcgagc gggagtggac agtatcgcgc cctagagcta ctgcgggccg
     6541 gcgtggtcgg cgtggttttg ccgctgttcg tgcctgcccg cgtgtccccg agcgggccac
     6601 ggctctcgga tctcgaaggc tcttatcagc gcgtgttcga cgcgctgacg cgaacccctc
     6661 cttacgcatt gccgggctgc ttggcgtcgc gtcagagcgt gcgcacgttc ttggctttcg
     6721 aaggcgcggc cccgctgctg gaacggcaag attcgctggt cgagttcgtg gcgcgtggcg
     6781 tgcgcagcat cgggctcgtg cacacgcgtg acaaccggct cgcggcgtcg tcgcagcagc
     6841 cgtccgagct ggcaggcact ccggcgggcc acaaagggct cacgaccgag ggggtcgacc
     6901 tcgtcgagcg ggccgtgcgt ttgcgcgtcg ccgtcgatgt atcccacgcc tctgacgcga
     6961 ccgtgcgcga ggtggcggag ctggtcacga gcgcccgggt gcccatgcta gcgacgcact
     7021 ccaactcgcg tgtggttcgc aatcatcccc gcaatttggc tgacgccgag gctctcgcga
     7081 tcgcgcgctc cggagaggta gtcggggtca acctgcattc cccattcttg gtggcggggc
     7141 ggcgggcgcg gatcgcggac gtcgtgcgtc acgtccgtta ctgggtccgc gtgctcggag
     7201 tggagcacgt cggcatcggt tctgatttcg agggcgacat tcgacctccc gaggggctcg
     7261 agagcgtcgc ggacctcccg aagcttgcgc tggctttgga gcagagcggc ttgtcccggg
     7321 cagcggtgga gcagattttc ggtctgaatg cgctgcgaat cctgtgcccc ccgtccgagc
     7381 gttgatcttc ggcgtgagcc cggtatagct gccgcaccgc atgtcgacct tggaagaccg
     7441 ttgcttggag gcgatcccgc attggctgag cctgctcggg gacgatgcgc gtgagctcgc
     7501 cgagctgttg ggcgtcgatg gcgctcctca cgtcaaaggc gcggcggcgc tcgggctcac
     7561 gtttctggtg cgttcgctcg agttcatctc cgaggtggag gaacagggct tcgccgaggc
     7621 ggcgatcgtg ctgcgagtcg cggcggaacg cgtgccggca gaggagctcg tcggcgagca
     7681 aaacagtgcc gcgcttcaga ctttggcgcg gctaggccaa gatgcggctt tggttcgcga
     7741 attcttgggc gaagaattcg agctgctgct cgctctgctc cacaatccgg cagctttcgc
     7801 gttgcatgga aagacttacg agcagctgct cgaagaaccc gaggcgctgg cggacttggc
     7861 ctatcgcgcg cggcagttcg ccgaccagta ttccgcgcca agctttgctc gcgacccgaa
     7921 gagcctgatc aaacttcgcg cgtacttgcg ggtgaagacc agccctggag cctgag
//
LOCUS       DASTKU010000700        12378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1025777, whole genome shotgun sequence.
ACCESSION   DASTKU010000700 DASTKU010000000
VERSION     DASTKU010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12378
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025777"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFQ61_24485"
     CDS             complement(<1..293)
                     /locus_tag="VFQ61_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018555222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET9957687.1"
                     /translation="MKPPMSASSLVPNVLAERYASAPMRRLWSPENKIRLERQLWVAV
                     LRAQRDLGIEVPPGTIEAYEAVIDRIDLSSIREREKKTRHDVKARIEEFCALAG"
     gene            complement(414..1097)
                     /locus_tag="VFQ61_24490"
     CDS             complement(414..1097)
                     /locus_tag="VFQ61_24490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957688.1"
                     /translation="MTAIAPPRVFVAFSQGASSAEVPVDARSTAYRVEFVRARRGQLP
                     FSPPEPGTEWQRLEASSPLGSAQGLCGVTQHVLYTSAAEREILTRTSAKESGPWAVLI
                     PIRKSAAWWALAQDQRLALFHARRADGSDQVGTGHPRSGTDGGTESGTERGHYSVGAR
                     YAHRIYRRLYHARYLTDGAWDFLTYFEFPDGERATFEALLADLRDERLNPEWAYVEAE
                     VEFWLRREN"
     gene            complement(1214..2089)
                     /locus_tag="VFQ61_24495"
     CDS             complement(1214..2089)
                     /locus_tag="VFQ61_24495"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9957689.1"
                     /translation="MLDMTVAVAGLLVGFVVGLTGMGGGALMTPVLVLLFGVPPLTAV
                     SSDLVASMIMKPVGGAVHWKRGTVHRPLVFWLMLGSVPSAFIGVALLKYLGSEQALQG
                     IVKHALGVALLVVVFGLVVRPLLQRKRQQEGEDEAPLQVRPIPTLLIGIFGGLVVGMT
                     SVGSGSLMIVLLLMLYPRIRLSELVGTDLVQAVPLVSSAALGHLLFGDFKFGLTASIL
                     VGSLPGVFLGALFSSRAKDHVIRPVLIVVLLASAMKLLGAGTVLVGCTAAVVTLFAVL
                     YGYSASQRMARQSAS"
     gene            complement(2177..3532)
                     /locus_tag="VFQ61_24500"
     CDS             complement(2177..3532)
                     /locus_tag="VFQ61_24500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HET9957690.1"
                     /translation="MSGHGQSSSKGVVTRLVQAAALVVIVGLLFAATRAAPELEARLG
                     SVAAVGFLLLAGTLLSELLETFGLPHLSGYLLAGILGGPHILHLVDHETVEQLSPINT
                     LALALIAIAGGAELRLDMLKQVSRSVAWALLLQCCIGLFVMVGSFLALSRFIPFAAEL
                     DALPLIGVALLWGTLAVSRSPSALLGILAQTRAKGVVATFSLAFVMASDVVVVVMMAV
                     AMMLARPLVSPAMGISLSDFAVLGHELLGSVALGTTLGLALALYLRLVGTNLLLILVG
                     LGFGLSDFMRYVNIDPLLSFLTAGFVVQNLSGQGRRLLEAVEQMGSVVFVVFFATAGA
                     HLDIPLLATLWPIALALCSIRATVTILTARLSSRLADDPPILRRWAWSSLISQAGLTL
                     GLSVVVARAFPTFGEGFRSLAIAAVAINEMVGPVLFKLALDRAQESESPAQRTSLVPP
                     S"
     gene            complement(3529..4800)
                     /locus_tag="VFQ61_24505"
     CDS             complement(3529..4800)
                     /locus_tag="VFQ61_24505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kef"
                     /protein_id="HET9957691.1"
                     /translation="MNAIGLLLGLLVLSYLGSILVSGRTIRGFGLPSGAEYLLLGFVF
                     GPSVLGVINGSLLEAFAPVLVVGTAWVTLCAGLGYGQVGSRPVRVPRAVVGILFAALV
                     AAGVSGAVFLGFSVARVGVSDVRLWTALAAGAVCSETTRHAVRWVAERHGARGDLSDT
                     IADYARASALVPAALLAVLFAAAPGHGLPESPLVERVGLTLAIGVLLGMVSALLLGRE
                     FRRDESWGILLGVSLLGIGIAARLGLSCVSTTFFLGLTVTLVSPHRSELKFMVAPTER
                     PVLLPVLVLAGASVHVTGQPAFLILVGCGLIGRLLMDLMRGAILSRAIPSARPAGSWL
                     GLGMLPIGAFSLSSALAYQLRVPGAGGAEILAFAAVSALLGELLGPLTLRRALTRAKN
                     IDSTRPPPEPARTSSANAPRSSGSTTRREPT"
     gene            complement(4797..6719)
                     /locus_tag="VFQ61_24510"
     CDS             complement(4797..6719)
                     /locus_tag="VFQ61_24510"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HET9957692.1"
                     /translation="MSSVRPAVFLLSFLLVPALGLAQEPGPEQRPSLNQQPSAAPAGE
                     QGGPAAQARPARSVRPSPASSAIDPAGDSSVAAPVTPIGPPPPPAEGAADPAPSSPAR
                     SDVAASPQPALPVEARPVPAAPELLPGPAPSVAVPRMAPVGASVSPSSSASTRAPEAA
                     ASGAVSKAALPGAAAVKLGDTLVFSLRTAYAGKSADDRARRAAKALLAALEDPRGNEV
                     RVERQGEVAVIFAGPTPIVQLIEEDARLAGDSSLDVHAGSVASAVRQALESERQRSRV
                     AKNVFSLSLVVFFALIAIYLMKKVGDVADRVRGWLEDHGDRVLTVRVQRIEIVRPAVL
                     KSSALVAVSLARGFGQFGIFYAWLVVVLSLFERTRGYTERLTGFVLTPLSQLMERLAT
                     ALPLLVVALLAALAVFVLVRFVGLFFAGVTRQETAVAWLPADLAAPTSVLLRVAIVIA
                     SLVFAAPIVTGDPDGALGRAGAIALISIGLAATPIVATGLLGSVVLFGRRLRVGQHVE
                     VSGSRGRISAINLMELRIETPTHEEVRIPHLLLLWRPIRGLGLQPRISVEICVAAGIL
                     PSRVSAVLERAALCVGSEPIIEIGQVTADGILYRVTVTCAALEGRSKLLSALLEALSE
                     DALPLGRIGGAAARPA"
     gene            complement(6790..8001)
                     /gene="serB"
                     /locus_tag="VFQ61_24515"
     CDS             complement(6790..8001)
                     /gene="serB"
                     /locus_tag="VFQ61_24515"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HET9957693.1"
                     /translation="MTQPEHADRIVIHIAGTDRPGVTARVMRIIAEENAELVDIGQSV
                     LHGYLMLSAIVNIPQNSRALRRILFEASDLGLRFEVSPFRGPEGGYVVGNAPALCVTV
                     LGPLADGVAAARITGMMAEMSMNIREMRSLSEQDLSGLELIADLLPGRAISDQDLAAM
                     RGAFLSQGMQLGVDLAVQKDDMYRRTKRLVCMDVDSTFVKGEFIDELAELVGCKEQVS
                     AITARAMRGELDFKQALAERVKLLAGLPLERAMTLLEKIELTPGADRFVHALRSLGFR
                     IGLVSGGFTVFVDQLKRRYGLDFAFANELEIENGKLTGRVRGTVVDGERKAQVLKDMA
                     QVYNCRLEQTVAIGDGANDISMLQTAGLGIAFRAKPKLQEVADMSLNHNERLDTLLYL
                     MGLNARDLREV"
     gene            8234..9514
                     /locus_tag="VFQ61_24520"
     CDS             8234..9514
                     /locus_tag="VFQ61_24520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957694.1"
                     /translation="MTRQTSFAGWLPVGCIALGLLLSACSSEDLTDAAQPAGSGGNAA
                     PGSSGAASGGASNSGGSGGVSGSPAADAVARLLPRDARLVSLYEPPLNFSATALAFDP
                     LRPGELWVTLRRFPTGAPCTEQDPTGCAALRGQVTLLVGATGPNPMAEQRRDGNAWHF
                     MRRPTSIAFGDNGNLATCGEARTDNYEDEVIDYSGPTLWSSDPSIFGATPQADQNGTH
                     LDMLHSTPYCMGIEHERGNIYFTFNGKLGAIDRYDFKEPHQIGGEDHSDGELYRYAEG
                     EFTRAPEIPSHLALDRERQMLYVADTGNGRVARLDITSGSMGDDVETNDPIHVHVSMV
                     GAIVDTFVKGAPLTAPSGVVVVSSNVFVTDNATSRIHAFDPQGHELASMETGLPSGSL
                     AGIAIGPDERLYLADLKNGRAYRVESSTGAGGAD"
     gene            complement(9562..10656)
                     /locus_tag="VFQ61_24525"
     CDS             complement(9562..10656)
                     /locus_tag="VFQ61_24525"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET9957695.1"
                     /translation="MTLAFAALFSAAAWAAGDQHSAAGSASKAAPPPAGEHLPNRAGA
                     SRAQAHESVSSGGSPGKGPLWSAGAPRSQTQPSKGGAFALASPPASAKRAESHPPAAH
                     AGRPSVNTPAHSAGEARHSSRPPGDAQAQASKAGPVEEFKIPGSCRQLLTVRSKHWDA
                     VSGELRRYVRASDSEWKLVGDVVPVNVGRRGMAWGRGLQGEPPIVRRKRDRDLRAPAG
                     VFALGTAFGYAPDLEQNAKKYPYLPIRANTACVEQIDSEHYNRIIDTSQTASKRWELR
                     SEMLRPDGLFRWGVVVRQNEPDPRPGAGSCIFMHIWRGPKQGTAGCTAMEPANIQEVI
                     HWLDPAASPVLVQLPEPEYEKYRSAWQLPP"
     gene            10874..11686
                     /locus_tag="VFQ61_24530"
     CDS             10874..11686
                     /locus_tag="VFQ61_24530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957696.1"
                     /translation="MTALPASLRTFLEVFSTSLSDVRFADIDAKTLEKSAAELDAAAD
                     RLAEIQAALEAARAELTEKTDSFVQLAQRAHAYARIYAEGNPELSAQLDALALPKAGR
                     RAKTEDSAKGSEVPSGEKSRSRKRAEAPQSESASSESVQSELALGTPPAEEQQRGRGQ
                     RAGAQAELGAADGAVAGGVDQEELEEELENAAESTDLAFDGADEFEESADEVEAAPAR
                     SRVTTSGRAANGRAAANGRAGSNSRAPSGRGATSTTNSQSSERKGRRGTASA"
BASE COUNT         2477 a         4063 c         3943 g         1895 t
ORIGIN      
        1 ccggcgagcg cgcagaactc ctcgatccgc gctttgacgt cgtgccgagt ctttttctcg
       61 cgctcacgaa tcgatgacaa atcgatgcga tcgatcacgg cttcataggc ttcgatcgta
      121 ccggggggga cctcgatccc caaatcgcgt tgtgcgcgca gcacagccac ccaaagctgc
      181 cgttccagcc ggatcttgtt ctccggcgac cagagccggc gcatcggcgc gctggcgtaa
      241 cgttcggcga gcacgttggg cacgagtgaa gaggctgaca tcggcggctt catgcgccga
      301 gcctcggcac gaagcaactc acagccgggc cgtgcgcgaa aatagctgaa attggcggca
      361 ccaattcggc gatttccttc gccttccaag cttgagaccg agccttatga gcctcagttc
      421 tctcgcctga gccagaactc gacctccgcc tcgacgtaag cccactctgg attcaaccgt
      481 tcgtcgcgca aatcggcgag caacgcctcg aaggtcgccc gctctccgtc cgggaactcg
      541 aagtaggtga ggaaatccca cgcgccgtcg gttagatagc gggcgtggta cagccggcgg
      601 tagatgcggt gggcgtaacg cgccccaacg gagtagtggc cgcgctctgt tcccgactcg
      661 gttcccccgt ccgtacccga tcggggatga ccggtaccga cttggtcgga cccatcggcg
      721 cggcgagcgt gaaatagagc gaggcgttga tcctgggcaa gcgcccacca cgcggcggac
      781 ttgcgaattg ggatcaacac tgcccacggg cccgattcct tcgccgaggt gcgcgtcagg
      841 atctcacgtt ctgctgcgct cgtgtacagc acgtgttgag tgactccgca caaaccctgg
      901 gccgacccca acggtgacga ggcctcgagc ctttgccact cggtgccggg ctccggtgga
      961 ctaaacggaa gctgcccccg ccgggcgcgc acgaactcga cccgataggc tgtgcttcgc
     1021 gcatccacgg gcacctcggc agagctggca ccctgggaga aggcaacgaa gacccggggc
     1081 ggagcgatgg ctgtcatgtc gcctcagctc gcctcacgcc ctgtttcccg agcgttccgg
     1141 gtgcgtctct agcgcgctga ttcggggcgg agcgacgccg gctgcaagcc gggcttgggt
     1201 agcgtttgga agctcagctg gcgctctgac gggccatacg ctgggacgca ctatagccat
     1261 agagcacggc gaacagggtg actacggccg cggtgcatcc aaccagcacc gtaccggcgc
     1321 caagcagctt catcgccgaa gcaagcagca caacgatcag caccgggcga atcacgtggt
     1381 ctttggcgcg cgaggagaag agcgcgccca gaaagacgcc cggcaagctg ccaaccaaga
     1441 tcgaggcggt gagcccgaac ttgaagtcgc cgaacaacag atgtccgagc gccgccgagg
     1501 acaccaacgg cacggcttga accaagtcgg tgcccaccaa ctccgagagg cgaatgcgcg
     1561 gatagagcat gagcagcagg acgatcatga gcgagccgga gccgaccgaa gtcatgccca
     1621 ccaccaagcc accgaagatc ccgatcagca gcgtaggaat gggccgcacc tgcagcgggg
     1681 cttcgtcctc accttcttgt tggcgcttgc gctgcaaaag agggcgcacc accaagccga
     1741 acaccacgac gagcaacgcg acgcccaggg cgtgcttgac gatcccttgg agcgcctgtt
     1801 ccgaccccaa gtatttgagc agcgcgacgc cgatgaatgc ggaggggacc gacccgagca
     1861 tcagccaaaa caccaaggga cggtggacgg tcccgcgctt ccagtgaacc gcaccaccca
     1921 ccggcttcat gatcatcgaa gccacgagat cgctcgatac ggcggtcagg ggaggaacgc
     1981 cgaacagcag cacgagaact ggcgtcatca aggctccgcc gcccataccg gtgaggccaa
     2041 cgacgaagcc caccagcaag ccagctaccg cgacagtcat atcgagcatc gggaggcagt
     2101 ctgaacgagc tatcccgact agtctagtag gaaacatggg ttttacataa aacccaatga
     2161 ttccatgtaa ttaaattcac gacgggggaa ctaggcttgt ccgttgagca ggtgattcac
     2221 tctcctgggc tcggtcgagc gcgagcttga acaggaccgg acccaccatt tcgttgatgg
     2281 cgaccgcggc gatcgcaagc gagcggaatc cctccccaaa cgtggggaac gccctggcca
     2341 cgaccacact caaccccaga gtgagcccgg cctgcgagat caaggacgac cacgcccagc
     2401 gtcggagaat gggcggatca tccgccagcc tgctcgacag gcgcgcggtc aggatcgtca
     2461 ccgtggcgcg tatcgagcac agcgccagcg cgatgggcca cagtgtggcg agcaacggaa
     2521 tgtcgaggtg ggcgcccgcc gtggcgaaga acacgacgaa caccacgctc cccatctgct
     2581 cgaccgcttc gagtaagcgt cggccctgcc cgctcaggtt ctggaccacg aaacccgcgg
     2641 tcaagaagga cagcaacgga tcgatattca cgtagcgcat gaagtcgctc aatccaaagc
     2701 ccaaaccgac caggatcaaa agcaggttgg tgccgaccaa acgtaggtag agggccagcg
     2761 ccagcccgag cgtcgtcccg agagccacgc taccgagcaa ctcgtggcca agcacggcga
     2821 agtcgctgag ggagatgccc atggctgggc tcacgagcgg acgcgcgagc atcatcgcga
     2881 cggccatcat caccaccacc accacgtccg aggccatcac gaaggccaag gaaaacgtgg
     2941 ccaccacgcc tttagcgcgg gtttgcgcca agatccccag cagggcagac gggctacgac
     3001 tcaccgccaa ggttccccaa agcaaggcga cgccgattag cggcaatgcg tcgagctccg
     3061 cagcaaaggg gatgaagcgg gagagcgcca agaacgaacc gaccattacg aacaagccga
     3121 tgcaacactg gagcaacagc gcccaagcca cgctgcgcga cacctgcttg agcatatcga
     3181 gccgcagctc cgcgccaccg gcgatggcga tcagggcgag cgccaaagtg ttgatggggg
     3241 agagctgctc gacggtctcg tgatccacca aatgcaggat gtgtggaccg cccaagatcc
     3301 ctgccagcag gtaccccgag aggtgaggca aaccgaaggt ttcgagcagc tcactcaaca
     3361 acgtgcccgc gagcagcagg aacccgacgg ccgcgacgct gcccagacgc gcttcgagct
     3421 ccggggcggc acgcgtggcc gcaaacagca gacccacgat cacgaccagc gcggccgctt
     3481 gcaccaagcg agtgacgaca cctttgctcg agctttgacc gtggccgctc acgtgggctc
     3541 cctcctcgtg gtgctacccg aactgcgcgg agcgttggcg ctcgacgttc gcgcaggctc
     3601 gggcggcgga cgcgtagagt cgatattctt cgctcgcgtg agcgcacgcc gtagcgtcag
     3661 cgggcccagc agctcaccga gcagcgccga aaccgccgcg aacgcgagaa tttccgcccc
     3721 gcccgcgccg gggactcgta gctggtacgc gagcgcggag gacaagctga acgctccgat
     3781 tgggagcatg ccgagcccga gccaacttcc cgcgggacgc gcgctcggaa tggctcggct
     3841 caggatcgcg ccacgcatca ggtccatgag cagacgcccg atgagtccgc agcccaccag
     3901 gatcaagaac gccggctgcc cggtgacatg aacggatgcg ccagcgagca cgagtacggg
     3961 caggagcacg ggccgctccg tcggagccac catgaatttg agctcactgc ggtgcggcga
     4021 aaccaacgtg acggtgagcc ccagaaagaa cgtcgtcgag acgcagctca acccgaggcg
     4081 cgccgcgatg ccgatcccga gcagcgacac gcctagcaag atgccccagc tctcgtcgcg
     4141 ccgaaactca cgaccgagca gtagagccga aaccatgccg agcagcacgc caatcgcgag
     4201 cgtcaatcca acgcgctcca ccaacggaga ctcgggcaag ccgtgacctg gcgcggccgc
     4261 gaacagcacc gccaatagag ccgctggcac tagcgcactc gcgcgcgcgt agtccgctat
     4321 ggtatcggaa aggtctccgc gagcgccgtg gcgctcggcc acccagcgta cggcatggcg
     4381 cgtggtctcc gagcagacgg ctccagccgc caaagccgtc cacagccgca cgtcggaaac
     4441 acccacacga gccacgctga agccgaggaa aacagcccca ctgacgcccg cggcaaccag
     4501 agccgcgaac aagatgccta ctaccgcgcg cggcacacga acgggtcgag agcccacctg
     4561 accgtagccg aggccggcac acaacgtgac ccaagccgtg ccgaccacga gaacgggcgc
     4621 aaaagcttcg agcaacgagc cgttgatgac cccgagcacg ctcggtccga atacgaagcc
     4681 gagcagcaag tattccgccc cggagggcaa gccaaagcca cggatcgtgc ggcccgacac
     4741 caagatactg cccaaatagg acagtacgag caaacccaag agcaggccaa tcgcgttcaa
     4801 gcgggcctcg ccgcggcacc cccgattcgc ccgagcggga gagcgtcttc gctcaatgct
     4861 tcgagcaacg ccgagagcaa cttggaacgg ccctccaaag ccgcacaggt gaccgtgacc
     4921 cgatacagga ttccatcggc cgtgacctga ccgatctcga tgatcggttc actcccgacg
     4981 cacagcgcgg cgcgctccag cacggccgac acccggctcg gtaagatgcc agcggccacg
     5041 cagatctcca ccgagatccg cggctgcaag cccaagccac gtatcggccg ccatagcaat
     5101 aataggtgcg gtatacgaac ctcctcgtgg gtgggggttt cgatgcggag ctccatcaag
     5161 ttgatggcgg agatgcggcc ccggctgccc gacacttcga cgtgctgccc cactcggagt
     5221 cgtcgcccga agagcaccac gctgcccaac agcccagtcg cgacgatcgg ggtggcggcg
     5281 agcccaatcg agatcagagc aatggctccg gcgcggccga gcgccccgtc agggtcgccc
     5341 gtgacgatgg gagcggcgaa gaccaacgag gcgattacga ttgcgacgcg cagcaatacg
     5401 ctggtcggcg cggccagatc cgccggcaac caggccaccg cagtttcctg gcgcgtcact
     5461 cccgcgaaga acaggcccac gaagcgcacc agcacgaaca ccgcgagcgc ggcgagcagc
     5521 gcaacgacga gcaacggaag tgccgtcgcc aaacgctcca tcagctgcga caacggcgtc
     5581 aacacgaagc cggtcaaacg ctcggtgtag ccgcgcgtgc gctcgaacag cgagagcacg
     5641 accacgagcc aagcgtagaa gattccgaat tgaccgaagc cccgcgctag actcacggcc
     5701 accagcgccg agctcttgag cacggcgggg cgcacgattt caatgcgctg aacacgcacc
     5761 gtcaagacgc gatcgccgtg atcctcgagc cagccccgaa cccggtcggc cacatcgccc
     5821 accttcttca tcaggtagat ggcgatcaac gcgaagaaga cgaccagaga gagcgaaaag
     5881 acgttcttcg cgacgcgact acgctgacgt tcggattcga gagcctgccg cacggctgac
     5941 gccacactgc ccgcgtgcac gtcgagcgag ctatcgccgg cgagccgcgc gtcctcttcg
     6001 atcagctgaa cgattggcgt ggggcccgcg aaaatgacgg ccacctcccc ttgccgctcc
     6061 acacgaactt cgttgccccg aggatcttcc agggccgcga gcagcgcctt ggctgcgcgc
     6121 cgggctcgat cgtctgcgct cttgccggcg taagcagtac gcaagctgaa gaccaaggta
     6181 tcgccgagct tgaccgccgc ggctcccggc agcgcggctt tgctcactgc acccgaagct
     6241 gcagcctcag gggcgcgagt cgaagccgag ctcgacgggc tcactgaagc tccgaccggc
     6301 gccatccgcg gcacggcgac gctgggagct gggcccggca agagctccgg ggccgcaggc
     6361 acggggcgcg cctcgacggg caatgcgggc tgaggcgagg ccgcaacatc gctgcgtgct
     6421 ggggagctcg gagcaggatc ggccgctccc tccgcgggcg gcggaggagg accgattggc
     6481 gtcaccggcg cggccaccga gctatcacca gccggatcaa tcgccgaaga cgcagggctc
     6541 ggtcgcaccg atcgagccgg cctcgcctgc gccgcgggcc ccccctgctc ccccgcaggg
     6601 gcagcgctgg gctgctgatt gaggctgggg cgctgctctg gaccgggctc ctgagccaac
     6661 ccgagcgcag gaacgagcag aaatgaaagc aagaagaccg cagggcgcac gctgctcacg
     6721 gtcgcagcct atcccagata ggcgtccgcg gtcgacccta cgcgagccta cacgcggcct
     6781 tgctcgagct cacacctcac gcagatcgcg cgcgttgagc cccatcaggt agagcaaagt
     6841 atcgagcctc tcattgtggt tgaggctcat gtctgccact tcctgaagct tgggtttggc
     6901 tcggaaagcg atgcccagcc ctgctgtttg cagcatcgaa atgtcgtttg cgccatcgcc
     6961 gatagcgacc gtttgctcca ggcgacagtt atacacctgg gccatatcct tgagcacctg
     7021 cgccttccgc tcgccatcca ccacggtgcc gcgcacgcga ccggtcagtt tgccgttctc
     7081 gatttcgagt tcgttcgcga acgcgaaatc gagcccgtag cgccgtttga gctgatcgac
     7141 gaacacggtg aacccgccgc tgaccaggcc gatgcggaaa ccgagcgaac gcagcgcatg
     7201 cacgaagcgg tcggccccgg gggtcagctc gatcttctcg agcaaggtca tggcgcgctc
     7261 caagggtagc cctgccagca gcttcacccg ctctgcgagc gcctgcttga agtcgagttc
     7321 accgcgcatc gcgcgggcgg tgatggctga gacctgctcc ttgcagccca cgagttcggc
     7381 gagctcgtcg atgaactcac ctttcacgaa cgtcgagtcc acatccatac agacgagccg
     7441 cttggtgcgg cgatacatgt cgtccttctg cacggcgagg tcgaccccga gctgcatgcc
     7501 ctggctcaaa aatgcacccc gcatcgcggc taggtcctga tcggaaatcg cgcggcccgg
     7561 caacaagtcc gcgattagct ctagaccgct caaatcttgc tcgcttaggg agcgcatctc
     7621 gcggatgttc atcgacatct ccgccatcat gccggtgatc cgcgcggcgg ccacgccgtc
     7681 cgcgagcgga ccgagcacgg tgacacacag cgcgggagca ttgccgacga cgtagccacc
     7741 ttctggtccg cggaacgggc tcacttcgaa gcgcaggccg agatcggatg cctcgaacag
     7801 gatgcgcctc agtgcgcggg agttctgcgg gatgttcacg atcgccgaga gcatgaggta
     7861 cccgtgcaga acggactgtc caatgtcgac tagctccgcg ttttcttcgg cgatgattcg
     7921 cattacgcgg gcggtaaccc cagggcggtc ggttcctgcg atgtggatga cgatgcggtc
     7981 cgcgtgctcg ggttgggtca tgggaacgaa tgcactccgt acgagaaggg ggtaacgaga
     8041 agagagggct agggcggggc tctgttacca gccacggcaa ggttgagcga gagacttttc
     8101 cggaaggtcg gttccggctg ggaaactccg ccgaaccccc acagaaatca aggcggccgg
     8161 ggaatttttg cggcccagcg gagcactggg gcacaccttt acaggcgccc ctgagagctc
     8221 cagattccga cgggtgacgc ggcaaacgag cttcgcgggc tggctcccgg taggttgtat
     8281 cgcccttgga cttttgcttt cggcgtgcag ctccgaggac ctgacggatg cggcccagcc
     8341 cgccggatcg ggagggaatg ctgctccggg cagctccggc gcagcgagtg gcggcgcttc
     8401 caactccggc ggttcaggcg gcgtgagcgg tagtccggcg gccgacgccg tggcacggct
     8461 gctcccgaga gacgcacgcc tggtatccct ctatgagccg ccgctcaatt tcagtgcgac
     8521 cgcgctggcg ttcgacccgc tgcgccccgg cgagctctgg gtgacactgc gtcgtttccc
     8581 gaccggcgcg ccgtgcaccg agcaggaccc aacgggctgc gcggccctgc gcggacaggt
     8641 gacgctgctc gttggcgcga ccggacccaa cccgatggct gagcagcggc gtgatggcaa
     8701 cgcgtggcac ttcatgcggc ggcccacgtc catcgctttt ggtgacaatg gcaacctcgc
     8761 gacctgcggc gaggcccgca cggacaacta cgaggacgag gtgatcgact actcggggcc
     8821 gacgctgtgg tcttcggatc cgtcgatctt cggggcaacg ccccaagccg atcagaacgg
     8881 aacccacctc gatatgttgc acagtacgcc gtactgcatg gggatcgaac acgagcgcgg
     8941 caacatctac ttcacgttca acggaaagct cggcgcgatc gaccgctacg acttcaagga
     9001 gccgcaccaa atcggtggag aggaccactc cgacggagaa ctctatcgct atgcagaagg
     9061 ggagttcacg cgggcaccgg agataccgag tcatctggcg ctcgatcgcg agcggcagat
     9121 gctgtacgtg gccgacaccg gcaacgggcg agtcgcaagg ctggatatca cgtcgggttc
     9181 gatgggtgat gacgtggaga ccaatgaccc gattcacgtg cacgtaagca tggtcggcgc
     9241 gattgtcgat accttcgtga agggggctcc tttgaccgca ccgagcggcg tggtggtggt
     9301 gagttcgaac gtgttcgtga ccgacaacgc gacgagccga attcacgctt tcgatccgca
     9361 gggccacgaa ctcgcgagca tggagaccgg cttaccgagt gggtcgctgg ccgggattgc
     9421 gatcggcccg gacgagcgct tgtacctcgc ggatctcaaa aacgggcgtg cttaccgcgt
     9481 cgaatcgagc accggcgcag gcggcgcgga ttgagcagtg aactcgccgc agggccccga
     9541 gctcttcgtg aggtgcccga gctatggcgg tagctgccag gcagaacgat acttttcgta
     9601 ctcaggctcg ggcagctgca cgagcaccgg tgacgccgcc ggatccaacc aatgaatcac
     9661 ctcttggatg ttcgcgggtt ccatcgcggt gcagcccgcc gtgccctgct ttggtccgcg
     9721 ccaaatgtgc atgaagatgc acgagcccgc gccgggtcta ggatcgggct cgttttgtcg
     9781 caccacgacg ccccagcgaa agaggccgtc cggtctcagc atctcgctgc gtagttccca
     9841 gcgcttgctg gccgtttgcg aagtatcgat gatgcgattg tagtgctccg agtcgatttg
     9901 ctcgacgcac gccgtgttgg ctcgaatcgg caggtaagga tacttctttg cgttctgctc
     9961 cagatccggc gcgtacccga aagccgtacc caacgcgaag acgccggctg gtgcgcgcaa
    10021 gtcccgatcg cgcttgcgtc tcacgattgg cggctcccct tgcaggcctc gcccccaagc
    10081 cataccgcgc cgccctacgt tgacgggaac gacgtctccg acgagcttcc actccgagtc
    10141 cgaggcgcgc acgtagcgac ggagttcgcc gctcaccgcg tcccaatgct tcgatcgaac
    10201 cgtcaggagt tgccggcagc tccccggaat cttgaactcc tccacaggac cggccttcga
    10261 ggcttgggcc tgtgcatctc ctgggggtcg cgacgagtga cgcgcctcac cagcagagtg
    10321 cgcgggcgtg ttgacgctcg ggcgcccggc atgggccgcc ggaggatgtg actccgcgcg
    10381 cttcgcgctc gcgggcgggc tggcgagcgc gaaggccccg cccttgcttg gctgagtttg
    10441 gctgcgcggc gcccctgccg accagagggg ccccttgccc ggcgaacctc cagagctgac
    10501 gctttcgtga gcctgcgctc tggacgcgcc cgcgcgattc gggaggtgtt cgccagccgg
    10561 cgggggagcg gccttggacg cgctccccgc agccgagtgc tggtcaccgg ccgcccaagc
    10621 cgcggcgctg aacagtgcgg caaacgccag ggtcacggat aaaccaactg caggctggcc
    10681 tctgaaacgg cttcggatcc gattgagcat caccatggcc atggaaaagg agcggcgcgg
    10741 ccttccttag caccgcattc caccttcact acggcgcgtt cgcgacaaag ctccgcctcg
    10801 gggcccagaa atcgtgcgtc atcgcacaaa gccgttggcg gaatgagttg ctgattgtat
    10861 gttcagcgcc gcgatgactg ctctgcctgc ctctttgcgc acctttctcg aagtattctc
    10921 cacttcatta agcgacgtac gttttgcgga catcgacgca aagacgctag agaagagcgc
    10981 cgccgagctc gacgcggcgg cggatcgcct agcggagatt caggccgcgc tcgaggcggc
    11041 gcgggcagag ctcacagaga agacggattc tttcgtgcag ctggcacagc gagcccacgc
    11101 ctacgctcgg atttacgcag aaggaaatcc cgagttgagc gcccagctcg acgcactagc
    11161 tttgccgaaa gcgggccgtc gcgccaagac cgaagattcc gctaagggct ctgaagtgcc
    11221 gagcggagag aagagccgct cgcgcaagcg cgctgaggcc ccgcagtccg aaagcgcgtc
    11281 ttcagagtcc gttcagtcgg agctggctct gggaacaccg cccgcggaag agcagcaacg
    11341 cgggcggggg cagcgcgccg gggcgcaggc ggagctagga gcggcggacg gcgcagtcgc
    11401 gggcggagtg gatcaagagg agctcgagga agagctcgaa aacgctgctg agtcgacgga
    11461 tttggccttc gatggcgcgg acgagttcga ggagtctgcc gatgaggtcg aggcagcccc
    11521 cgcccgttcc cgagtgacca ccagtgggcg cgccgcgaat ggacgcgcag ccgcgaatgg
    11581 acgcgcgggc tcaaactcgc gagccccctc cggacgcggg gcaacgagca cgacgaatag
    11641 ccagtcgagc gagcgcaagg gccggcgtgg cactgcgtct gcctgaacga acgtatcgaa
    11701 ttagccgcgc ggaggctcag cagagacggc atttcgcacc tggcgcgtgc ggtgaacagc
    11761 ggagctcgga agagcttgcg ccatcaaggt ttcgcgcgcg gcgtcagcca atctgaagct
    11821 gctctcgcgt gggccttcgt gcgtcgtcgc gcaaaatcca ggttggctca caatagaaca
    11881 cgcggtcaga ggttggtatc cggctcatga agtccgttgt ctcgtggcat tgctagggat
    11941 tcggtcgtcg cggcggccgc aatcacacga ccgcatcgcc ccggcggctc gccctcaatc
    12001 acacgctcgg ttgggtgatt gagaagagtt cgtgtttctg tcacgcgagt tctcacaatg
    12061 cgcgagagcc caattctatg ttctcgatgc agccgaattc gagtcgcagc ccgcatcgga
    12121 tttcttcgct ttttgcatct gggaaccggt ccccaataaa agtgagccgt cgcctatcgg
    12181 cgagttcgaa atagcgcact tgggacgact ggagtcgaag tgcggtccgc gcctgcacga
    12241 tgagcactcg cgaggtccgt tgccttcaga aggcggttgc gtttcgaagg tcgagctctc
    12301 accatgcggc agccctctag cagtgtgagt aagaaaggcg tcttgatgaa gagcgtccgc
    12361 aatccggttc tcactggc
//
LOCUS       DASTKU010000701         5245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_356447, whole genome shotgun sequence.
ACCESSION   DASTKU010000701 DASTKU010000000
VERSION     DASTKU010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356447"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1797)
                     /locus_tag="VFQ61_24535"
     CDS             complement(235..1797)
                     /locus_tag="VFQ61_24535"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9957697.1"
                     /translation="MLKPGDTFERYTIEALIGQGGMGCVYRAYDPRLDRRVALKVISE
                     GAEDPATNARLLREARAAAAFDHPNAVTIFDVGEVDGAPYIVMELVEGRTLREAPDDA
                     PLPVATRVAQLADVARALAAAHKRGLVHRDIKPENVMVRGDGMVKVLDFGIARRTGGP
                     TNPQSPTQPALPTLTVEGVHLGTPVYMAPEQMRGEKLDGRADQFAWGVLAFELLAQKL
                     PWRGDDVLAVMASALTDPVDRLALEKAGVPPAVTSVVLRALEKQPAARFASMDELATA
                     LESAGVAETFGEPLKAKPAASATMAQQFSTSEVREVLAQAIERQASKQDSVKLGFEDL
                     IDIAAEVGVDVESLREASRALRVRGQEQLTVSAEATKRDAWLRHRRLAFYRHAGIYAI
                     VNAALLVLGLALLSFTPWWIWCLPALGWGVGVAIHGLVALTSNEEDWNEHNTAMQWWL
                     GNQQRGLPLPRGAALTDHAGVLHHGPRVRIGVAEALPSNADELADGDAPDADADADAR
                     AGSRLRTRAERN"
     gene            complement(2001..3566)
                     /locus_tag="VFQ61_24540"
     CDS             complement(2001..3566)
                     /locus_tag="VFQ61_24540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957698.1"
                     /translation="MTKRTRLLAISLLSFPTGLSLAACTGGNGSAGSGGSSSPSGGAA
                     AKGGTTASSGGTTTGSGGTTASSGGNTSNSGGSSNSSGGATNSSGGATSSSGGSTSSS
                     GGNTASSGGKTGSGGTAGGGGTSTASGGMTGSAGSAGGSSTGQCTLPKFPDASSLPVN
                     KKLPDPFTFFDGTKVTTKAQWECRRQEILAMAAKYIYDPVPTKPDEVTGTVSGGTVSI
                     TVKVGSKSDTFSATISGSGTAIALELSGGIFPSSHKKLSFATGFENKIKSLYGFSANV
                     NTNIANGWMIDRVMDVLEKNPSSGHDPTKIMVSGCSGCGKGAYLAGVFSRAPLVVIVE
                     SGGGGAANLRQAEWFRHGDGKSVWKCADDVPQSIDNLESNGICGPWITNTAQWLVSSP
                     SKVNNLPFDTHLLLATIAPRYLLHFSNNNGVNSWCHLGGTCEALSAWAAKPVWKALGV
                     PERMGFHMYSAGHCSNPAEATALAGEFFKRAIDGNMSAKTDVMDIMDNGVQQPVSEWK
                     TMWVDWNMDTVLQ"
     gene            complement(3916..4554)
                     /locus_tag="VFQ61_24545"
     CDS             complement(3916..4554)
                     /locus_tag="VFQ61_24545"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9957699.1"
                     /translation="MLKVYGFSRVNEFARGNTRDLRVLWALEEMGIPYEIVGMDHPNH
                     DLDSASFRAKNPFGQIPVIDDDGVVVTESGAIVLYLARKSGKLMPRDLAGEAQVLRWS
                     VAALNSIEVPILTKWFVDMNGAKGTKPSEALRQWSELRLGQLDGWLANRQYVATDDFT
                     VADILMTHVLGSRSTLGLTEPYANVLAYLERCTARPAWKRTFDAYCERVKAG"
     gene            4916..>5245
                     /locus_tag="VFQ61_24550"
     CDS             4916..>5245
                     /locus_tag="VFQ61_24550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957700.1"
                     /translation="MKQLFPLFVLAAAVSSGCAASTELDGEQLGADDSVGDVEQAFTE
                     AACPTLTPDRLISVVGHNKSATARSSGTNYGTDTRCPRAFRVDVQAINAATASVYVYW
                     QSPFPQSK"
BASE COUNT          884 a         1695 c         1672 g          994 t
ORIGIN      
        1 tgccttggcc gttccgcggt cggtcgattt caggtggtcg aggccatcac tcgtgagttc
       61 cccgaagtag accacggtat cgtcgaaccg cgtgaccgac acgggcgtgc gcggtggcgc
      121 cgcacagttt acgaagagcg cggccagaac tacgcttctg ctgctcgaca ctcggtcgaa
      181 actatcgacg cggcgcggtc agggcaagcc ggcggggaag acgacgatgg cgcgtcagtt
      241 gcgctcggcc ctagttctga gccgggaacc cgctcgcgcg tcggcgtcgg cgtcggcatc
      301 gggcgcgtca ccatcggcta gttcatcggc gttactgggc agagcttccg cgacaccgat
      361 tcgtacccga gggccgtggt gaaggacgcc ggcatggtcg gttagagccg cgcctcgcgg
      421 caagggcagg ccgcgttgct ggtttccgag ccaccactgc atggcagtgt tgtgctcgtt
      481 ccagtcctcc tcgttcgacg tgagcgcgac gagcccgtgg atcgccactc ccacacccca
      541 cccgagcgcg ggcaagcacc agatccacca cggcgtgaag ctgaggaggg cgaggccgag
      601 caccaggagc gcggcgttga cgatggcata gattcccgcg tgccggtaga aagccagtcg
      661 tcggtgccga agccaagcgt cgcgtttggt cgcttcggcg ctgacggtca actgttcttg
      721 gccgcgcacg cgaagtgcgc gactggcttc gcgtagcgac tcgacatcca cccccacctc
      781 tgcggcgatg tcgatgaggt cttcgaagcc gagcttgacc gagtcttgct tcgacgcttg
      841 ccgctcgatg gcctgagcga ggacctcgcg cacttcggag gtcgagaact gctgcgccat
      901 ggtcgcgctc gcggccggct tagcctttag aggctcgccg aacgtctcgg ctacgccggc
      961 ggactcgagc gcagttgcga gttcgtccat cgaagcgaat cgagccgcgg gctgtttttc
     1021 gagcgcgcgc agcaccacgc tcgttacggc cgggggcacg ccggcttttt cgagcgccaa
     1081 ccgatcgacc gggtcggtca gggccgaggc catcaccgcg agaacgtcgt cgccgcgcca
     1141 gggtagcttc tgcgcgagga gctcgaaggc gagcacgccc caggcgaact gatctgcgcg
     1201 cccgtcgagc ttttcgccgc gcatctgttc gggggccatg tacaccggcg tgccgaggtg
     1261 aacgccctcg acggtgagcg ttggcagcgc cggttgagtg gggctctggg gattggtggg
     1321 tccgcccgta cgtcgtgcga taccgaagtc gagaaccttc accatgccat cgccgcgcac
     1381 catcacgttc tcgggcttga tatcgcggtg gacgagccct cgcttgtgcg ctgcggcgag
     1441 cgcacgtgcg acgtcggcga gctgcgccac gcgcgtcgcg actggcagcg gtgcgtcgtc
     1501 gggcgcttcg cgaagcgtac gcccttcgac gagctccatc acgatgtatg gcgcaccgtc
     1561 cacctcaccc acgtcgaaga tcgttaccgc gttcgggtga tcgaaagccg ctgcggcgcg
     1621 cgcctctcgc aacaacctcg cgttggtcgc ggggtcctct gctccctccg aaatcacctt
     1681 gagggccacg cgacgatcca aacgagggtc gtaagcgcgg tatacgcacc ccatgccgcc
     1741 ctggccgatc agggcctcga tggtgtagcg ctcgaaagtg tcgcccggct tcagcattga
     1801 ggcgggagag cgtaccgcat tcgagcgagc gggtacaaac gtggaccggc gagccccggc
     1861 gaaggtgcgc tgtccctcta cccagtcccc tactattggg gcggcgaaga tcgctgccgt
     1921 gcatccttcg cacacggcag cgatccaaag cctcgaagca agactttttc tctcagcgtc
     1981 gcgtcagctc aatacgccga tcactgcagc accgtgtcca tgttccagtc gacccacatg
     2041 gtcttccact cgctgaccgg ttgttgaacg ccgttgtcca tgatgtccat cacgtcggtc
     2101 ttcgcgctca tgttgccgtc gattgcccgc ttgaagaact cacccgcgag tgccgttgct
     2161 tccgccgggt tgctgcagtg ccccgcgctg tacatgtgaa atcccatgcg ctccgggacg
     2221 ccgagcgctt tccacacggg cttcgcggcc caagccgaga gcgcttcgca ggtgccgccc
     2281 aggtgacacc acgagttcac tccgttgttg ttcgagaagt gcagcaagta ccgcggcgca
     2341 atcgtcgcga gtaacaggtg cgtatcgaac ggcaggttgt tcaccttgga ggggctcgat
     2401 acgagccatt gagccgtatt cgtgatccag gggccacaaa taccgttgct ctcgaggttg
     2461 tcgatgctct gcggcacgtc gtccgcgcat ttccaaacgg acttcccgtc gccgtgccgg
     2521 aaccattctg cctgtcgcaa attggccgcg ccgccaccgc ccgattcgac gatgaccacg
     2581 agaggagcac gactgaatac acctgcgaga taggcaccct ttccacagcc ggaacacccc
     2641 gacaccatga tcttggtggg atcatgcccg ctgctcggat tcttctccaa cacgtccatg
     2701 acccggtcga tcatccaccc gttggcgatg ttcgtgttca cgttggcgga gaagccgtaa
     2761 agcgacttga tcttgttctc gaagccggtc gcgaaagaga gcttcttgtg agatgagggg
     2821 aagatgccgc ccgacagctc gagagcgatc gccgtgccgg aaccgctaat cgtggccgag
     2881 aacgtgtctg atttcgagcc gactttgacc gtgatcgaga ccgtcccgcc cgacacggtt
     2941 ccggtgactt cgtctggctt ggtgggaacc gggtcgtaaa tgtatttggc cgccatcgcc
     3001 aaaatttctt ggcggcggca ctcccattga gccttcgtgg tgactttcgt tccgtcgaag
     3061 aacgtgaacg gatcggggag cttcttgttc accggcaggc tgctcgcgtc ggggaacttg
     3121 ggcagcgtgc attgcccagt cgagctgcca cccgcgctac cggcgctgcc cgtcatgcct
     3181 ccggacgcag tgctcgttcc gccgccgcca gccgtgccac cgctgcccgt tttgccgccc
     3241 gagctcgcgg tgttgcctcc ggagctcgag gtgctcccgc ccgagctgct ggttgcgcca
     3301 cctgagctgt tggtcgcgcc ccccgagctg ttgctcgaac cgccggagtt gctggtgttg
     3361 cctccggagc tcgcggtcgt gccaccagag ccggtagtcg tgccgccaga gcttgcagtt
     3421 gtgcccccct tcgctgcggc tccgccgctc ggcgaggagc tgccaccgga gcccgcgctg
     3481 ccgttgcccc cggtgcaagc ggcgagcgag agaccggtgg ggaaggagag gagggagata
     3541 gcgagaagtc tggttctctt ggtcatagtg ctcactggtc actttgggat cgctagagga
     3601 cgatcccgtg atggaagttg agaggaggcg tgactgcgct cagagggacg cccgacgcac
     3661 taattcccga gctcttggtc tcgcgatcac gagtgggaag gattcgcgca gtcattgcag
     3721 tctggtcacc tctttctctt gaggttggac ctctgatgat gcacgaccgc gtgcgcgctc
     3781 ccttcggtcg ctagaggcga agcgagcgca acagcgtgcg cgagaacgtg tgcggtcggt
     3841 gctccagcaa cgcgcggcgt tcgcggtgct cgtgagagtg cgctcgccgc gctgtccggc
     3901 ggggagcgaa gccgttcaac ccgcttttac gcgctcgcaa tatgcgtcga acgtcctctt
     3961 ccaagcaggg cgcgcggtgc agcgctctag atacgcgaga acgttcgcgt acggctcggt
     4021 gagccccagg gtgctccggc ttccgagaac atgcgtcatc aggatgtccg caactgtaaa
     4081 gtcgtcagtc gcgacgtact gcctgttcgc tagccagccg tcgagctggc cgaggcgcag
     4141 ctccgaccac tgacgcagcg cctcgctcgg cttcgtgccc ttcgcgccgt tcatgtcgac
     4201 gaaccacttg gtcaggatcg gcacctcgat cgagttcagc gcggcgacgc tccaacgcag
     4261 cacttgcgct tcccccgcca ggtcacgagg catcagcttg ccgctcttac gcgcaaggta
     4321 gagcacgatc gcgccggact cggtgacgac cacgccgtca tcgtcgatga ccgggatctg
     4381 cccaaacggg ttcttggccc tgaacgaggc cgagtcgagg tcgtgattcg gatggtccat
     4441 tccgacgatc tcgtacggga tgcccatctc ttccaacgcc cataggacgc gcaggtcccg
     4501 cgtgttgccg cgcgcaaatt cgttcactcg ggagaagccg tagaccttga gcatgccggc
     4561 atcctagtgc agggcatgtg agattgcatc aacatcgccg gcgctgcagt agagcggacg
     4621 cggagggctc gccgccgggc gtgatagctg ttcactcctc gccgcttgcc ggactgcagc
     4681 gacgtaccaa agaagcgcag cttcccgacg tgcgcgtcga cgcgccggcg cggtactctc
     4741 ttgggacgca gttgcttgtc gccgtcgagt ttggggcagt gagctcacga actagcattc
     4801 tcgtgcgcaa agctcgaacc caaactcgaa gtcttgcgtt gatcgagtcg tgagcacgag
     4861 tggttcgtcg aagcgcgcgc cacgagctca cctcaacgcg aaaggtgaag agacagtgaa
     4921 acagctattc ccactatttg tcttggccgc ggcagtctcg agcggttgcg cggcgagcac
     4981 cgagctagac ggagagcaat tgggcgccga tgattcggtt ggcgatgtcg aacaggcgtt
     5041 caccgaagct gcgtgcccga cactcactcc cgacaggctc atctcggtcg tcgggcacaa
     5101 caagagtgcc acggcgcgct cgtccgggac caactatggc accgacaccc gttgccccag
     5161 ggcgttcagg gtcgatgtcc aagccatcaa tgcggcgacc gcctccgtct acgtctactg
     5221 gcagtcgccg ttcccccaga gcaag
//
LOCUS       DASTKU010000702         8529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_580204, whole genome shotgun sequence.
ACCESSION   DASTKU010000702 DASTKU010000000
VERSION     DASTKU010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580204"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="VFQ61_24555"
     CDS             complement(<1..1080)
                     /locus_tag="VFQ61_24555"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9957701.1"
                     /translation="MTLTGAASEEQSSPDARGVSESVGESQPSPVETVEASLTGVPLP
                     PMVPGLPVLGHALELAGDSRPFLIGLYRKFGPIFRLRVPNQTLTVLAGPEANQFFSQA
                     GGEHFRSRELWQAFATELGSDHLMNAIDGPEHAWQRKVMKPGFARQTMLDRLPEVIRV
                     VDQACAKLAIGREIAVLPFMQGVVTEQLGVVLTGRSPGEYMPDIRKMVRLALNTLVVR
                     KWPRAVLWSPGYRRAKARVRELANGLIADHRALAPPARKADMIDDLMAAHQQRPDVYT
                     ENDLRVGVVGPYIAGLDTVANTCAFMLRALLSEPEVLRSVTEEVDTAMNAGPLTAEVL
                     KQMHALHGAAMETLRLYPVAALSQRT"
     gene            complement(1610..3259)
                     /locus_tag="VFQ61_24560"
     CDS             complement(1610..3259)
                     /locus_tag="VFQ61_24560"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HET9957702.1"
                     /translation="MHSRVIGPIRVAALLAWMAAGCSQGASNAPALPDVNPPTAPAQE
                     SDSNPEEMPVSNLQNEERDYELDTNPDLPNPERGVAFWFETAATDPHTLEYHFLWLGE
                     VCDQPLIWNGLDGVGTSAVLKDWATAAIELRKKGVKVIFRPRYDSPGREGLPNGCQKL
                     EGASYGRMLEHVDAIAKMLGDPQIKPSLAFIEMGYLGSWGEWNTGDNCDVSKVATCQR
                     LAPVLLANEPVQDRIHLIQYVVEAYRKAGVTRSVAVRRPEYHRNAVENMKLPKTSLGF
                     HNDCLMVDENDGGNFIALRWDYADYHELYPDTAFNSVTEAKAYLAAQVGTGSMGGETC
                     PNASKTEPWRNANAVRERLQTDQFQYLHAAFAPEFRAAMIAGLAWDDVKSRLGYRYHA
                     KSVRYPSSVRAGAPLTVSVDLENSGFARIPQERTAYLVLEGAAQAGGGVAAYVVGKSN
                     PGSSFTPLLAIAQAGENVGLWRPGETITFSQTFPAPGVAGSYRLKLYIPDPECIDNRF
                     CGPAQDRLLAVRLATKRAGNSVFDPNAGTNDLDVSITVSPP"
     gene            complement(3696..5654)
                     /locus_tag="VFQ61_24565"
     CDS             complement(3696..5654)
                     /locus_tag="VFQ61_24565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957703.1"
                     /translation="MSKSVLWVAASMALLVACSSGEKVSTPGGSGAGGQSGSNSPTGG
                     APLASGAASTGTNTVGVSGATSSSGATGATQAGGAPGVGGSGGFGGVASSASGGQIIG
                     LGATSGAGGSSFGSGGFTSPLETAGMANSGGSSTTPPECQPGEKRCAGAEPILQSCGA
                     DGHFQSTSCEFVCRSGGCTGVCKPGERRCSDTQPQRCNDFGEFVADGPACNAQCEKGT
                     CTGSCRNGATQCTTSSSLQTCRDGAWSPSSECEFTCVNGACGGVCDPGSRRCFSGTQS
                     ETCSSTGEWGNRTSCNFVCVNNTCGGECRPGDKRCTGTNLETCNNQGQWAVSESCPNA
                     CVNKACGGECRPGSKRCGAANASLETCDGNGKWVQSTCPGACANGSCVTCTQGATECV
                     GTTKIRTCGSNNQWGQATECQFACVGNACGGACKPGARECTAPGAARYRACNDQGSWS
                     TTTCSDTQSCKAGSCGSYPRVVFVTSEVYNGNLGGIAGADKKCQDLAKKANLTGTFKA
                     FLGEPGKPLRDRMNAEGGPFVLVDKKTVVAYNFNDLDSDSLNPVKQTEKGLKPPATVM
                     PPLQAAQDRLITSCDEFVSGLVWVNSLGVSSESRDICKGFTFAGNSEVVDDANFGSWL
                     STEDWTFVCSPTRTGLCATKAPLFCIEQ"
     gene            complement(5790..7736)
                     /locus_tag="VFQ61_24570"
     CDS             complement(5790..7736)
                     /locus_tag="VFQ61_24570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957704.1"
                     /translation="MTDLGTERSARACFGTMLYRGMRFWAPPDWCAGLGMLGLASVLG
                     LGACQVSDKSDYRFEREPAMPQAGAMGGNSTTARAGEIGGASRGGTANAGSGSTGVSA
                     GAGNAGNAQGGSTPNPEFTGTAGIGAAGTNNVTGTPECQPGSKRCDSSARAVHTCDAN
                     GHWLTQACEFVCAEGACVGACLPGQRSCRDLTPQRCDDRGAWVPAGDPCAAQCIEGAC
                     SGACQEGSKQCVSSDQVQECRGGQWTQGSACPFACLDQGCGGECRPSDTRCASSTEME
                     TCGAIGAWGFKAACDFVCSGKKCGGQCRPGAKRCASPTELQSCDSSGEWTGVSACAYA
                     CVNDACGGVCKPRTKRCGASGKTVETCSATGQWEAQSCPEACVDGACSSCTPGETECV
                     SSSQVRACNAAGQWEAATTCANACVTNQCGGVCKPGARQCVGNASTPQYQICSASGQW
                     GAATSCAPEPACKAGSCGQDPKLVFVTSSLYTGNLGGLSGADAKCNERARAAGLSGTF
                     RAFLSDGTGSPATRFSKQGGPYRRVDNVVVAQNFNDLTANGLSKLINLTEKGSAPPAA
                     KPPASRSDICGPDTSNLVWSNTLRSGSVAVSDGGCSNWTTTNSGKTNFGRFDDLQHWS
                     SWCTSTGGDNPQCAAQAPLYCFEQ"
     gene            complement(7855..>8529)
                     /locus_tag="VFQ61_24575"
     CDS             complement(7855..>8529)
                     /locus_tag="VFQ61_24575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957705.1"
                     /translation="RVIKVAAVSGAARVTLRYIASQSGAAEWAIDVPNQSLGMVLSGL
                     ESGSTQVPADARAGMVTSVVIPDDPHTVPVANAQLILDRWPRPNWPEASALTGASGAI
                     SLRVAPDSAVGSFRVTRLEVAAALFALSGAGSLDFRSGLRLRAQARGSRSCQELAALL
                     PASVYREKVRAYVGSHTKDERVELSLSIDLQDTAARENAEQTGLRFAWHLAAGCGLEE
                     LRPDPV"
BASE COUNT         1550 a         2907 c         2460 g         1612 t
ORIGIN      
        1 cgtgcgttgg ctcagcgctg ccaccggata gagccgcaga gtttccatcg cggctccgtg
       61 cagagcatgc atctgcttga gcacctccgc cgtgagtggg ccggcgttca ttgcggtatc
      121 cacttcttcc gtcacactcc gcagcacctc cggttcgctc aacagggccc gaagcatgaa
      181 cgcacacgta tttgcgaccg tgtctagccc ggcgatgtag ggccccacga ctccaacacg
      241 cagatcattc tccgtgtaga cgtcaggccg ctgctggtga gctgccatca agtcatcgat
      301 catatccgcc tttcgggctg gcggcgccag cgcgcgatga tctgcaatca gcccattcgc
      361 cagctcgcgc acccgcgcct tcgcgcgccg ataacccgga gaccagagca cggctcttgg
      421 ccacttgcgg accacgagtg tattcaaagc cagccgaacc atcttgcgaa tatccggcat
      481 gtactcgccc ggcgatcgcc cggtcagcac gacccccagc tgctcggtca ccacaccttg
      541 catgaacgga agcactgcaa tctcgcgacc gatcgcgagt ttcgcacaag cttggtcaac
      601 gacgcgaatg acctctggca gccgatcgag catggtttgc cgagcgaagc caggcttcat
      661 cactttccgc tgccacgcgt gctcgggtcc gtcgatcgca ttcatcaagt gatccgaccc
      721 gagctcggtc gcgaacgcct gccagagttc ccggctccga aagtgctcgc cgcccgcttg
      781 cgagaagaac tgatttgctt ccgggcccgc caacaccgtc agcgtctggt tcggcacgcg
      841 gagcctgaaa atcggcccga atttgcgata cagcccgatc aggaacggtc gcgaatcccc
      901 ggcgagctcg agcgcatgtc ccaacaccgg tagccccggc accatcggcg gcagcggtac
      961 acccgttaga ctcgcttcga ccgtttcaac ggggctcggc tgactctcgc caacgctttc
     1021 gctcacgcct cgagcatccg gcgaactctg ctcttcgctt gctgcgcctg tcagggtcat
     1081 ggttcggttc ctctctccct cgtgagtgtc tccgagtttc ccgacctcac cgttcgacaa
     1141 ggctttgaat atcagctctg agtcgggctg tgaacgccgt ttgtgggcgg ccacgtcatt
     1201 acgagcgcgc tcacccggcg agagtcccca cctgcgcagc cccctttgct ccaagtttca
     1261 ttctccaccg cgcaagccgc ccctaataac tccgtctccg cagcgtcagg acccacttgg
     1321 ctagggggcc acttggcact ccacgcaaag acgcacactt tcccgctcca aaagcaaaac
     1381 ttcatgggcc cgcgaccggt tcggcagcgt aagccgtctt tcgaaggcaa gcgagcgacc
     1441 ggcgccccca cacacgactg ggttcagacc ggcgctcgcc gttgcagggt tggcgagcct
     1501 acggttgaac atgggaggtg agaagcgcgg gagggcgtcg taagcggaag gcgccggtcg
     1561 ctcgcaaaag tgatagcttc gaaggtttac cgagtgatcg tcggaagctt cagggcgggc
     1621 ttacggtgat ggaaacatcg aggtcgttgg tgcccgcatt tggatcgaag acactgtttc
     1681 ccgctcgctt cgtcgcgagt cgcaccgcga gcagcctgtc ctgcgcgggg ccgcagaaac
     1741 gattatcgat gcattctgga tctggaatgt agagcttgag gcgatagctc cccgctacgc
     1801 ccggagccgg aaaggtctga ctgaaggtga tggtttctcc cggtcgccag aggcccacgt
     1861 tctcccccgc ttgagcgatg gccagcaacg gggtgaatga gcttcctgga ttcgatttgc
     1921 caactacata cgcagccaca ccgccgcctg cctgcgctgc cccctcgagc acgagataag
     1981 ccgtacgctc ctgggggatt cgcgcgaatc cgctgttttc gagatccaca ctcacggtga
     2041 gcggagcgcc cgctcgaacc gagctgggat agcgaacgct cttggcgtga tagcgatagc
     2101 ctaggcggct cttgacatca tcccaggcga gtccggctat catagcggcg cggaactcgg
     2161 gcgcgaacgc cgcgtgcaaa tactgaaact gatccgtctg gaggcgctcg cgaaccgcgt
     2221 tggcgttgcg ccaaggctcc gtcttggagg cgttcggaca ggtctcccca cccatgctgc
     2281 cggtgccaac ttgcgcggcc aagtaggcct tggcctcggt gacgctgttg aacgcagtgt
     2341 cggggtagag ctcgtggtaa tctgcgtagt cccagcgcag ggcgatgaag ttgccgccgt
     2401 cattttcgtc gaccatcaag cagtcgttgt gaaagcccaa gctcgtcttg ggcagcttca
     2461 tattttcgac cgcgttgcgg tggtactcgg gtctgcgcac ggctacgctg cgggtcacac
     2521 ccgcttttcg gtacgcttcg acgacgtact gaatgagatg aatgcgatcc tgcacgggtt
     2581 cgttcgcgag aagcaccggg gccaatctct ggcacgtggc aactttggaa acatcgcagt
     2641 tgtcgccagt attccactct ccccagcttc cgagatatcc catctcgatg aacgccaaac
     2701 tgggtttgat ctgcggatct ccgagcatct tcgcgatggc atcgacgtgt tcgagcatgc
     2761 gtccatagga ggcgccttcg agcttctgac agccattcgg tagtccctct cttcctggac
     2821 tgtcgtagcg cggcctgaag atgaccttta cccctttttt ccggagctcg atcgcggcgg
     2881 tagcccagtc tttcaggacc gcgctcgtgc ccaccccgtc aagaccattc cagatcaggg
     2941 gctgatcgca cacctcaccc agccaaagga aatgatactc gagggtatgc gggtccgtgg
     3001 cagccgtctc gaaccagaac gcgacgccac gctcagggtt ggggagatcg ggattggtgt
     3061 ctagctcgta atcccgttct tcgttttgca ggttgctgac cggcatctct tcgggattgg
     3121 agtcgctctc ctgcgcgggc gccgttgggg ggtttacgtc aggtagagcc ggagcattcg
     3181 aagctccctg ggagcacccg gccgccatcc aagccaacaa ggccgctact cggattggac
     3241 cgatcactct cgaatgcatt gcggtctccg ctgaaacaag aaccttcact cgtgttgaat
     3301 ctggcacttg cctgtactgt gtaaggcgcg cccgaaacgg gtgattcagc acgaataaag
     3361 cgggccgctt cggcacgact caaccgccgg aggcgctcga cggccgggct caaacggccc
     3421 ttcccagcgg caatcccgcg acaacgacgc ttcgaacggg tggccatcgc agcccgtcgc
     3481 cgaccacggc ctccaggcta ggcccggcac gacttcggtc gcgcggctgc ccgtcgccga
     3541 gttgtccgga atcgcggcag gaccctccga aggatccgct gtatcgagat gggccgagta
     3601 ctgatgggcc cgagctcttt tcccggaggg tcgttcatct gccggtttgt agcaccgggg
     3661 ttctacgggc gcgtgaaagg gcggctaagc gcccttcatt gctcgatgca aaagagtggg
     3721 gccttggtgg cacaaaggcc cgtgcgcgtg ggtgagcaaa cgaaggtcca atcttcggtg
     3781 ctcagccaag acccgaagtt cgcgtcgtcc acgacctccg aattaccagc gaaagtaaag
     3841 cctttgcaga tatcgcggct ttcgctgctc acgccgagcg aattgaccca taccaacccc
     3901 gacacgaact catcacaact cgtgatcaga cggtcttggg ccgcctgaag cggcggcatg
     3961 accgtagccg gtggcttgag gcccttctcg gtctgcttca ccgggttgag cgagtcactg
     4021 tctaggtcgt tgaagttgta agcgacgacc gtcttcttat ccaccagcac gaacggcccg
     4081 ccctcggcgt tcatgcggtc acgcagcggc ttgccgggct cacctaggaa ggctttgaac
     4141 gtgcccgtga gattcgcttt cttggccagg tcctggcact tcttgtcggc accagcgatt
     4201 ccacccaagt tgccgttgta aacctcagaa gtgacgaaaa cgactctggg atacgaacca
     4261 cagctgcccg ctttgcacga ctgagtatcg ctgcaagtcg tcgtcgacca cgacccttgg
     4321 tcattgcacg cgcgatagcg agctgccccc ggcgcagtgc actcccgggc tcctggcttg
     4381 caggcgcccc cgcaggcatt ccctacacaa gcaaattggc actctgtcgc ctgtccccac
     4441 tgattgttgg agccgcacgt gcggatcttg gtcgttccaa cacactcggt cgcgccctgg
     4501 gtgcacgtga cacaggaacc gttcgcgcac gcgcccggac aagtcgattg cacccacttg
     4561 ccgttgccat cgcaggtctc gagcgacgcg ttcgcagcgc cgcaacgttt cgagcccggt
     4621 cggcactctc ccccgcaggc cttgttcaca cacgcgttgg gacagctctc ggacaccgcc
     4681 cactggccct gattgttgca agtctctagg ttggtgccgg tgcaacgctt gtcaccaggt
     4741 ctgcactctc caccgcaggt attgttgaca cacacgaagt tgcaggacgt tctgtttccc
     4801 cattcgccgg tgctgctgca cgtctccgac tgggtgccgg aaaagcaacg tcggctaccc
     4861 gggtcgcaaa cgcctccgca ggcgccgttc acacaggtga actcacattc actcgatggc
     4921 gaccaagcac cgtctcgaca ggtctgcaga ctgctcgagg tcgtgcattg cgtcgccccg
     4981 ttgcggcagc tgccggtgca agttcccttt tcacactgcg cgttgcaggc cgggccgtcc
     5041 gcgacgaact caccgaagtc gttgcaacgc tgaggctgag tgtcactaca gcgccgctca
     5101 cccggcttgc aaacgccagt gcatccgcca cttctgcata cgaactcgca cgaagtactc
     5161 tgaaaatggc cgtccgcgcc gcagctttgc agaatgggct cggcaccggc gcagcgcttc
     5221 tcacctggtt ggcactcagg aggcgtcgtc gagctaccac ccgagttggc catccccgca
     5281 gtttccagag ggctggtgaa gccgccgctg ccaaaactgc tcccgccagc gccgctcgtt
     5341 gcgccgaggc cgatgatttg gccgcccgag gcgctcgagg cgactccgcc gaacccacca
     5401 cttcccccga cgcccggggc accgccagcc tgcgtcgccc ccgtggcccc cgagctgctg
     5461 gtcgctccag agacccccac cgtgttcgtc ccggtgctcg ccgcaccgct tgccaacgga
     5521 gccccgcccg tcggagagtt ggatcccgac tgaccgcccg cgcccgatcc gcctggcgtg
     5581 gacaccttct ctcccgaact gcacgccact agcagagcca tcgacgctgc gacccaaagc
     5641 acagatttcg acatacacaa actcacttgg ttgaaacccc aaaccaccct gaaatcgtcc
     5701 tgcgcatcaa accgtcaaac gcggagatag tcttgcactt ggctgcagcg tcgcagaaca
     5761 cgctcttcgc tcgacctcga ttgagcgcgt cattgctcga agcaatagag cggcgcttgt
     5821 gcggcgcact ggggattgtc cccgcccgtc gaggtgcacc aactcgacca gtgttggagg
     5881 tcgtcgaaac ggccgaagtt cgtctttccg ctgttggtgg tcgtccagtt cgaacaaccg
     5941 ccatccgaca ctgcgaccga gccacttcgc agcgtattgc tccacaccag gttactggta
     6001 tccggaccac agatatcgga gcgggacgcc ggcggttttg ccgcgggcgg cgcgctgccc
     6061 ttctcggtca agttgattag cttggataag ccattagccg tgaggtcatt gaagttctgc
     6121 gcaacgacca cgttatccac ccggcggtat ggtccgccct gtttggagaa gcgggtagcg
     6181 ggagaaccgg tgccgtcgct caagaacgca cgaaaggtcc cgctcaggcc cgctgcgcgc
     6241 gctcgctcgt tgcacttcgc gtcggccccg ctcaacccgc cgagatttcc cgtgtacagg
     6301 ctggacgtca cgaacacgag ctttgggtcc tgcccacaac tgcccgcctt gcacgccggc
     6361 tccggtgcac agctggtcgc ggcgccccat tgaccggacg cgctacaaat ctgatattgc
     6421 ggggtactgg cgttgcctac gcattggcga gcgcccggct tgcacacgcc accacactga
     6481 ttggtaacgc aggcattcgc gcaggtggtg gcggcctccc attgccctgc agcattgcag
     6541 gctcgaactt gagaactcga aacgcattcc gtctctcccg gcgtacacga agagcaggcg
     6601 ccatcgacac atgcttcggg acagctctgc gcctcccatt gaccggtcgc actgcaggtc
     6661 tcgacggtct tgcccgaggc cccacagcgt ttcgtgcgcg gcttgcacac gcctccgcac
     6721 gcgtcattca cgcaagcgta agcgcatgcc gacacgccag tccactcgcc gctcgagtcg
     6781 cacgattgca gctccgtggg tgacgcgcag cgcttcgcgc cgggtcggca ttggccgccg
     6841 cacttctttc ccgaacaaac gaagtcacac gccgccttga agccccacgc cccgatggca
     6901 ccgcacgtct ccatttcggt gctcgacgcg cagcgcgtgt cagacggacg gcattccccg
     6961 ccgcagccct ggtccaagca cgcaaacggg caggctgaac cctgagtcca ctggccacct
     7021 cgacactcct gaacttgatc gctcgagaca cactgcttgc ttccctcctg gcacgccccc
     7081 gagcaagcgc cttcgatgca ctgtgcggcg caagggtccc ctgccggtac ccacgcgcct
     7141 cgatcgtcac agcgctgtgg agtcagatca cggcagctgc gctggccagg gaggcacgcg
     7201 cccacgcagg ctccctccgc gcagacgaac tcgcaggcct gagtgagcca atggccattt
     7261 gcgtcacatg tatgaactgc ccgtgcgctc gaatcacagc gcttgctgcc gggctgacat
     7321 tcgggtgtac cggtgacgtt gttggtgccc gccgcgccga ttccggctgt gcccgtaaac
     7381 tccggattcg gtgtgctccc gccctgggca ttgcctgcgt tgcccgcgcc cgcgctcacc
     7441 ccggtcgacc ccgatcccgc gtttgcggtg ccaccgcgag aagccccgcc gatctcaccc
     7501 gcacgagctg tagtcgagtt cccgcccatg gcgccggcct ggggcatggc gggttcacgt
     7561 tcgaaccggt agtccgattt atccgaaact tggcaggccc cgagccccaa caccgaggcg
     7621 agccccaaca tgccgagccc ggcgcaccaa tccggtggcg cccagaaccg cattccccga
     7681 tacaacatcg tgccgaagca cgctcgcgcc gagcgctctg tacccagatc cgtcattgaa
     7741 ccacgccgca cctctaagcg tgttctggag acgaagattg tatccgattg cgtgccccgc
     7801 actcagtcga gattcacgta gtccggaacg ctccctcgaa agaccgccgc aatctcatac
     7861 cggatccggt cgcaactctt ccagtccgca gcccgcagcc aaatgccaag cgaaacgcag
     7921 tcctgtctgc tccgcgttct cacgagcagc agtatcctgg agatcgatac tcaggctgag
     7981 ttcgacacgc tcatccttcg tgtgggagcc aacgtacgcc cgcaccttct ctcgatatac
     8041 cgaggcgggc aagagcgccg cgagttcctg acaagagcgg ctgcctcgag cctgcgcgcg
     8101 aagccgcaat ccgctccgga agtccaagct tcccgcccca ctcaacgcga atagtgccgc
     8161 cgcaacctct aaccgtgtca cccgaaagct gccaacagcg gagtccggag caaccctcag
     8221 cgaaatggct cccgaggcgc cggtcagcgc gctggcctcg ggccaattgg gccgtggcca
     8281 acggtcgagg atcaactggg catttgcgac cgggaccgtg tgaggatcgt ctggaatcac
     8341 cacgctcgtg accatccccg cccgagcgtc cgccggcacc tgagtggacc cgctctcgag
     8401 ccccgagagc accatcccga gcgattgatt gggcacgtcg atcgcccact cggcggcgcc
     8461 ggactggctc gcgatatagc ggagggtgac ccgagcagcc cctgagacgg cagccacctt
     8521 gatgacccg
//
LOCUS       DASTKU010000703         3563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1250386, whole genome shotgun sequence.
ACCESSION   DASTKU010000703 DASTKU010000000
VERSION     DASTKU010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250386"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            939..2495
                     /locus_tag="VFQ61_24580"
     CDS             939..2495
                     /locus_tag="VFQ61_24580"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HET9957706.1"
                     /translation="MTTKSMDKIMALAKRRGFVYQASEIYGGLNGFWDYGPLGAQLKK
                     NLREAWWQDVVMSPCGGAPGPFGEPVRMVPLDTCIIQHPRVWEASGHIAGFNDPMVDC
                     RETKARYRYDHLMVYAPESGDRQGKPLFCYMPDTPSQQKQRKKAEKAFGAVVEVPLTS
                     LSKDAFEHILAPDAEKVGSLTEPRAFNLMFKTYIGATATENDVAYLRPETAQGIFVQF
                     KNVVDTTRVKLPFGIAQTGKAFRNEVTPRNFTFRSREFEQMEIEFFCHPSEAFEWYRF
                     WRDTRVAWWKSLGLAGENLQLREHDKDELAHYAKDGAGTSDVEYRFPFTAPGFGELEG
                     IAHRSSFDLTQHQKFSRTNLEYFDAERGELLPNGSRKGEKYIPHVIEPSAGLDRGVLA
                     LLCEAFTEDASRPSPELMKLHPRVAPIKAAVFPLVNKDGMPEISEKLHAELARRFNRV
                     GFIEHDVKQTIGKRYARMDEAGCPFCFTVDQNTAPTQQVTVRDRDTGAQQNVALDRVG
                     DWLAEKLQVG"
     gene            2636..>3563
                     /locus_tag="VFQ61_24585"
     CDS             2636..>3563
                     /locus_tag="VFQ61_24585"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET9957707.1"
                     /translation="MLRSASFAWFSSVSSRLTRSVAACSALLTLGYFAACTPASPNSA
                     AHPASDTQNSSASGAYKSIAYRNVFLEHGKSQAEIDAKLRLAYNTYFTPTDPNTLYFE
                     MGPDMAYIKDILHDDIRSEGQSYGLMIAVQQNDQARFKKLWKFAFEKMRVKDPKHPSY
                     RCFAWVVDPKGEMCDENSAPDGEEYFAMALYFAHNRWGSAAGEGYDNYKHWADDLLDA
                     VRHRQPISGNRSRRMDQGKDDGNPCTGTSTPEPVTSVALFDEAAKMVRFNGTQGENYT
                     DPSYHLPAFYELFAEFGPKADSAWWKEVAVTSR"
BASE COUNT          779 a         1108 c         1048 g          628 t
ORIGIN      
        1 cccgccgttc caacgtcacc cgtgggtcga tccgtgattg gctcggcgcc aacattacag
       61 ccgaacccgc ccagcaacgc ggccgcgaag gcgaaccgaa gcttccgacc gaggcgaggg
      121 agcgaagcgg acgcgctatc gaattcactc atcgacacac catgacctct tgagaaaaca
      181 tccatgcggc ctttctgaca acgacacccc cgaggacgta gattagcgtc gcagatgcgc
      241 gaggaaaggt ccccttgtcg gttttagcag ccttttagca acaatatttt tggagttgcc
      301 ggaactttcc gagcaccaca caaaaactga cggaaccgac cacccagcat gtcggcaatc
      361 acttcaaagg gtgtaacccg cgggccaaaa accccgggat tttccgagca ttttgcatca
      421 gagcccgcga gacgagtgat ggccagattg cccactgcag ggccgtcgaa caccaacccg
      481 agacgcttaa ttcgagtcga cggcggacga ggcactgcaa agttggagtt cgcgctttga
      541 catgccggaa cccgccggcg caacgcgagc agcccagttt ggtacgggcg agcgccagca
      601 attcgccgcc tggaacggaa gcactgcgga tcaagagcta ggcggttcga gcgcccgcgt
      661 ttgagtggtg gagcgccgag ctcgggctgc ggcgtcgaat agcccaatga cggaggccgc
      721 tcctaacgcc ctgaaatggg tgaaaaaagg ccttctccgg gtgccctcga gcttgccagc
      781 tccgagatcc ttggcctttc tgctacgctg cgcacgctct tggctgtgtg gccgagactc
      841 aagcatacag accccactgc aacttcaatt cagcgttcca gccgcagccc agccttcatc
      901 actcagtatc agtgaatgaa actcgagaag ataagccgat gaccaccaag tcgatggaca
      961 agatcatggc gctcgcgaag cgccgcgggt tcgtttatca agcttccgaa atatacggcg
     1021 gcttgaacgg tttttgggac tacggcccgc tcggcgctca gctcaaaaag aatctacggg
     1081 aggcctggtg gcaagatgtg gtgatgagcc cttgtggcgg cgcacccggg ccattcggcg
     1141 agcccgtgcg gatggtgcca ttggacactt gcatcattca gcaccctcga gtctgggaag
     1201 cgagcggcca tatcgcgggg ttcaacgatc caatggtcga ttgccgcgaa accaaagccc
     1261 gctatcgcta cgaccacctc atggtctatg ccccggagag cggggatcgc cagggcaagc
     1321 cgctattttg ctatatgccg gatacgccga gccagcaaaa gcagcgcaag aaggcggaaa
     1381 aagcctttgg tgccgtcgtg gaagtgccac tcactagcct gtcgaaagac gccttcgagc
     1441 acatcctggc tccagacgcg gaaaaggtag ggtcccttac cgagccgcgc gccttcaatt
     1501 tgatgttcaa aacctatatc ggtgccaccg ccactgaaaa cgacgtggcc tatctgaggc
     1561 ccgagacggc acagggcatc ttcgtccaat tcaagaatgt ggtggatacc acgcgggtga
     1621 agctgccgtt cgggatcgcg cagacaggca aggcgttccg caacgaggtg acgccgcgca
     1681 acttcacctt ccgaagtcgc gagttcgagc agatggaaat cgagttcttc tgccacccct
     1741 cggaagcttt cgagtggtac cgattctggc gcgatacccg cgtggcctgg tggaagagcc
     1801 tgggcctggc cggcgaaaac ctccagttgc gcgagcacga taaagacgag ctcgctcatt
     1861 acgccaagga cggagccggc acgagcgacg tggaataccg gttcccgttt acggcgcctg
     1921 gtttcggtga gctcgagggc attgctcacc gctcgagctt cgatctcact caacaccaga
     1981 agttctcgcg caccaacctg gagtacttcg acgcggagcg cggtgagctc ctgccgaatg
     2041 gctcgcgcaa aggcgagaag tacatccctc acgtgatcga gccctccgcg ggtctggatc
     2101 gcggtgtgct cgcgctgctt tgcgaagcct tcaccgaaga cgcttcgcga ccgagccccg
     2161 agctcatgaa gctgcaccct cgcgtggcgc cgatcaaggc agcagtattc ccgctcgtca
     2221 acaaggacgg catgcccgaa atcagcgaga agctacacgc cgagctggcg cgccgcttca
     2281 atcgcgtggg tttcatcgag cacgacgtca agcagacgat cggcaaacgc tacgcacgca
     2341 tggacgaggc gggttgcccg ttctgtttca ccgtggatca gaatacggcg cccacgcagc
     2401 aggtgacggt acgcgatcgc gacacgggcg cccaacagaa cgtcgcgctg gatcgcgtgg
     2461 gtgactggct ggcggagaaa ctgcaagtcg gctgagctgc aaggcggctg acgccagcct
     2521 catgcccgct gagcccacgg acccgctgag ctcgcgcatg cctcccgttc gatactcaaa
     2581 aagaaaacga gtctgagaag acactcccga gacattggtg gagaaagagc ctcccatgct
     2641 gcgttctgct tcattcgcct ggttttcgtc tgtttctagc cgcctcaccc gctccgtggc
     2701 cgcgtgctcc gcgctgctca cgctgggcta tttcgcggcc tgcacgcctg cgtcgccgaa
     2761 cagcgcggcc caccccgctt cggacactca gaacagctcc gccagcggcg cttacaagag
     2821 catcgcttac cgcaacgtgt tcctcgagca cggcaagtcc caagccgaga tcgatgccaa
     2881 gctgcgcctc gcctacaaca cctactttac gcccaccgat ccgaacacgc tctacttcga
     2941 gatgggaccg gacatggcgt acatcaaaga catcttgcac gacgacatcc ggagcgaggg
     3001 tcaatcctac ggcctgatga tcgcggtgca gcagaacgac caagctcgct tcaagaagct
     3061 ctggaagttc gcgttcgaga agatgcgcgt gaaggatcca aagcacccga gctatcgctg
     3121 cttcgcttgg gtggtggatc ccaagggcga gatgtgtgac gagaactcgg ctccggatgg
     3181 ggaagagtac ttcgcgatgg cgctctattt cgcccacaac cgttggggca gcgccgcggg
     3241 cgaaggctac gacaactaca agcattgggc cgacgatctg ctcgacgcgg tccgccatcg
     3301 gcagcccatt tcaggcaacc ggagccgccg catggatcag ggcaaagacg acggaaatcc
     3361 atgcaccgga acgagtacac ccgagccggt cacctcggtg gccttgttcg atgaggcagc
     3421 caagatggtg cgcttcaacg gcactcaggg cgagaactac acggacccct cgtatcacct
     3481 gccggccttc tacgagctgt tcgccgaatt cggacccaag gcagattcgg cgtggtggaa
     3541 agaagtcgcc gtgaccagcc gtg
//
LOCUS       DASTKU010000704        12386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1474263, whole genome shotgun sequence.
ACCESSION   DASTKU010000704 DASTKU010000000
VERSION     DASTKU010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12386
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474263"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..630)
                     /locus_tag="VFQ61_24590"
     CDS             complement(31..630)
                     /locus_tag="VFQ61_24590"
                     /inference="COORDINATES: protein motif:HMM:NF037003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957708.1"
                     /translation="MLARFAEHREHVAHWVRHQRQLVLRRLVTVIDQNAPLYKYAGPG
                     HWNDPDMLEVGKLANETEDRAHFSMWAIMAAPLIAGNDIRNMSAATRSILTNSEVIAI
                     DQDPLGEQGRVVATPGSNLQVWSKTLSAPNARAVALFNRGTSAASISVQWSSLGLPAG
                     AATVRDLWSHMDLGSFTDSYTATSVPSHGVVMLKVVSAK"
     gene            complement(540..1772)
                     /locus_tag="VFQ61_24595"
     CDS             complement(540..1772)
                     /locus_tag="VFQ61_24595"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HET9957709.1"
                     /translation="MMIQLEAAIATDLRSALSVPANILTPDSGSAAQACRNPVAHRHL
                     ERRSSWATLIFALEILGCSSPKDAPITPAVAAGTSEPRSSGGTYGSGGAPGGAGAVGG
                     AGVSGGSSAGGTFHTGGIAGGASSGGPTTASGGSAMGGTMATSGAGAAAGGATAVGGG
                     SALGGAPPSGGSSSLAGQSAGGSSASGGSTSTSGGSTAAAGNAGTGGMAPLASTPPMG
                     WNSWNTFGCANLNEDLIRGVADRMVSSGMKDAGYQYVNLDDCWMNGRDASGKIQVNTS
                     KFPSGMPALVRYVHEKGLKLGLYSTPGTKTCAAIYGGYTGGSGSLGHETSDAKTYASW
                     EVDYLKYDKCTAALSGFAVMRDALRATGRPIFYSINPGDGTGCVPGKCSLDLLSTANM
                     WRIGFDINASWSSVVSLL"
     gene            2519..3910
                     /locus_tag="VFQ61_24600"
     CDS             2519..3910
                     /locus_tag="VFQ61_24600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957710.1"
                     /translation="MTHRSGLIFKGPISCMFLVIAGCSGGGSSGVLTGAQGGGSSGGA
                     AQGGQSSGNSSGASQGAQGGVNSTGSGGSTNASGGSGLTGGQTASTNGGSAQAGGQST
                     AASGGDAGRSGSGGTPAGGSSGKGGGTASGGLAGAASGGAGTMAGGQSGENGGQAGRG
                     GGAGQTGTAGASGGAENPCVGKAWPSADPTNAGPFEVTSEKNVGPLAGALPDPVYGNE
                     QQRFNIYRPKDLAQSGYCHPVLVWANGHGDNPEQNPPKCIVNSATNTWCGTYPMLINQ
                     LASHGFVVIASLSTTTSQGNPLPTIAGLNWLLQQAEDPMSPYYHRLDTAHIGALGHSE
                     GGMSTCKAAADPRIKAIATVSGTTSLSGLHGPALFFCGEKDEVAKCSGINTVFKSVKD
                     QPSIFINNLTADHGSWLGQNGIKGPTIFGLTAWFRIHLMGDTENRKRFYGQNCTFCTD
                     NRVKVEQNSLMTQ"
     gene            4381..6255
                     /locus_tag="VFQ61_24605"
     CDS             4381..6255
                     /locus_tag="VFQ61_24605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HET9957711.1"
                     /translation="MRHQRQLALATWLLALVTALPSTSYADNPIVQTNYTADPAPMVH
                     EGRLYLYTSHDEDNTVSNFYTMNDWRLYSTVDMVNWTDHGTPAAGKTFSWFTDNAWAP
                     QGISRNGKFYLYLPINNNTGAKIGVAVANSPAGPFSDPLGKGLAAQGNGNIDPTVFID
                     EDGQAYMYWGNASLRYVKLNSDMTSYSGSVNTVSLNGFLEGPWFYKRGSLYYMVYAAN
                     GTSTEKISYATSNSPTGPWTVRGDVMDSGKTYTNHPGVVDFKGHSYFFYHNSALPGGN
                     DFRRSVCVEEFTYGTDGSIPKLTMSTKGPNGIATLNPFQQVEAETIAFSAGLKTEVCS
                     DTGGGMNVSSISNGDYIKVKDVDFADGVTSFDARVSNSGSNAKIELHLDSQTGTSLGT
                     CDVSGASSWTTKTCMVTGGSGKHDLFLKFTGGSGDLFKFNWWKFTGPTMPNNGTGGTG
                     GVGGTGGAGQGGVAGQGSGGVSGGGGTSTAGGGNASGGAGGMTASGGSTAGGAAGNGQ
                     GGTGTAASGAANGGSVSAGSGGTLAMMGGSSNGAKGGATPSGGTASSSGGSAGNPQVT
                     GGLSSTGPGAAPPVDSSGCSCRVGADQRQAGPWATLGLLGTVLLSRKRSAARKRARST
                     "
     gene            complement(6296..7072)
                     /locus_tag="VFQ61_24610"
     CDS             complement(6296..7072)
                     /locus_tag="VFQ61_24610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957712.1"
                     /translation="MRLSRTRRLRHDICALGCAGALAWTGTARAYRPFTGTDAAIASP
                     GEVEIEFGPAALIRQGQDWRLSAPAVVLNYGVSSQLEAILEGRHDVRLGKRSEEPRSQ
                     LEDSAVSLKMMLRNGSLQDGTGLSIAIEAGALLPSPDRKLGTHVASIVSMRWPEFTIH
                     VNVANDVLLAPRQYEAMLDVIAEGSERVRVRPVTEILVSREFGNGKFSRALTGSLLIG
                     LIARYDDSLSIDCALRGGRAPERFEEELRAGLTWSFEVTR"
     gene            complement(7069..8157)
                     /locus_tag="VFQ61_24615"
     CDS             complement(7069..8157)
                     /locus_tag="VFQ61_24615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957713.1"
                     /translation="MRPAKFLPLLFGVLAAGFTSAGCTCNAHPAPPSVSRGDFAESSS
                     NRAREVPLGLGQGLGFDDLVYSSEMGKTIAPAGRSNCVALLDPNSLAASNICSGRAEA
                     TAYSGGHDSGLTSADFGAGMVFAVDRSNRAVLALHPSTGEVVASAKLAGSPDYVRWIR
                     KTEDVWVTEPDAESIEVFHWSSGSAPQLIRRRAIQVSGGPESLVVDQDSAVAYSHLWK
                     GRTLALDAQTYAITDTWENGCQGSRGIALDSERHYLFFGCAEGQVGVLDLQHAGRRLG
                     TARCSPRVDLVSVNLRLGHLYAPAASDGTLCIFEVSPHGQLTKLGTLSAVRGAHCVAN
                     DERGGVWVCDPARGSLLHFRDPYPPAPR"
     gene            8294..8962
                     /locus_tag="VFQ61_24620"
     CDS             8294..8962
                     /locus_tag="VFQ61_24620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9957714.1"
                     /translation="MRVLVVDDHLETRQLLLRNLQLDSHSVKAVDTCAEAEVQLSRGD
                     FDVVVLDIMLPDGSGLELCSRLRMSQMNAPILLLTARGDVKSRVLGLESGADDYLPKP
                     FAVSELRARVKALGRRGPIARGQTIARGGLEVDLDARRVRVDGRALPLTARELAIIEL
                     LATRRGRVVQRDELIESVWGELTDSARSSLEVLVARIRRKLGRHAHALRTVRNLGYVF
                     EVEE"
     gene            8959..10266
                     /locus_tag="VFQ61_24625"
     CDS             8959..10266
                     /locus_tag="VFQ61_24625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9957715.1"
                     /translation="MKPTTIAARVSSAGARTTVTALTLVALSTSLAVTELLTAKTDQL
                     LNAMGERTAYYLEARPAPIDWDWIRAEVDEARPSEVRVEIRDPTGHTRAAYGPRTPIL
                     AFHRNCRDDGKLRVCGRRSEHFLVLAAIDRSDDLAARNRLLFVIGALSALAAGTVLLV
                     SRSLMRVAVKPLTDLAARVRAVQPGSGERVELRSDLVEVNELAGRFDDLVERFEQALA
                     REKRFAAVASHELRTPLTVIQAEIEALPKNDATSRALLSVQRLNQLVEVLLWFARAQS
                     GLGGERMEWINVADLIRDQVSALQIANPSARFSVHLPDEALVHGDEHLLGRVTANLLD
                     NAVKYGEGSEVSVSASHGSVDLEIEVTNGGAGIPSDVREHVFEPFFRVPGRSADGFGL
                     GLAFARAVARAHGGDLTLSPHSQSLTRFVLTLRLASWSETEHG"
     gene            10336..11136
                     /locus_tag="VFQ61_24630"
     CDS             10336..11136
                     /locus_tag="VFQ61_24630"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9957716.1"
                     /translation="MNASANSGRLTQPYPVFGLLLLLVSVLLIAAAPPTRPNIVDGII
                     GDQDPTPEVSTAELRRAVAELGAIVLDARPYEEYAVSHLPAARSVKGKVGTTPALYVA
                     DVNDVLQQVPDKKQPLILYCNGLFCGRSKRFAAELVQAGYTNVRRYQLGIPAWRALGG
                     VTQVEKDALMSLLARDQTAVLVDAREPGQRRPRLLGATPIPLSETTQAKDDGRLPMTD
                     HNTRIFVVGSSGTQARAVAEAIVKDAFHNVAFFDGPETELPRLLDRHR"
     gene            complement(11289..12074)
                     /locus_tag="VFQ61_24635"
     CDS             complement(11289..12074)
                     /locus_tag="VFQ61_24635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957717.1"
                     /translation="MMRTAIALFFLVCGLPATAAAQPAPQPLPQPPRPQQPSSAVGQP
                     ALPAVPPGLDPKFRPDPGILLLLAPVPTGAQKAAGEGLATLRELVTAQNCVGLGFDKP
                     EDVQRAKLGVPIRDLSVSLERLKQYEANTSIAALVLPFEKFTVPVLVDGAVRSSISVV
                     RRGPEWHAASFGFPNLIRGISGVVAAQAGNAQARGISIVRVPSLRLLLVAHVTNGRLF
                     FTEVTGGRRLRLDPGTTQPADSLLFSLRGPARAYRPLRPRPDR"
     gene            complement(12071..>12386)
                     /locus_tag="VFQ61_24640"
     CDS             complement(12071..>12386)
                     /locus_tag="VFQ61_24640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957718.1"
                     /translation="YGFHATEWPGPLSWTEVKEQLSREDHCQRTPFLFTWEWAEGGSH
                     MMVAKGYSSVKGKNYIEILDPWAPCQGDEGIVDYASFASEPGSHTHSLDFYDIEYVGF
                     GS"
BASE COUNT         2369 a         3849 c         3906 g         2262 t
ORIGIN      
        1 gccctcaatc ggtcgtgggc aaccctcgag tcacttggca ctcaccacct tgagcattac
       61 gacgccgtgg ctcggaaccg aagtggccgt ataggaatcg gtgaaggacc ctagatccat
      121 atggctccag agatcccgca ccgttgctgc tccggccggc aaccccaggg aactccactg
      181 cacgctgatc gacgcggcgg acgtgcctcg attgaagagt gcgaccgctc gcgcgttggg
      241 agccgacaat gtcttggacc agacttggag attcgagccg ggagtggcca ccacccgacc
      301 ctgctcaccc aaggggtctt gatcgatcgc gatgacctcg gagttggtga gaatcgatcg
      361 ggtcgcggcg ctcatattcc gaatatcgtt ccccgcaatc agcggcgcgg ccatgatcgc
      421 ccacatgctg aaatgcgccc gatcttcggt ttcattggca agcttgccga cctccaacat
      481 atcggggtcg ttccagtgcc ccggaccggc atacttgtag agcggtgcat tttgatcgat
      541 tacagtaacg agacgacgga ggaccagctg gcgttgatgt cgaacccaat gcgccacatg
      601 ttcgcggtgc tcagcaaatc gagcgagcac ttgccaggaa cgcagcccgt gccgtcgccg
      661 ggattgatgc tgtagaaaat agggcgaccg gtggcgcgca gcgcgtcgcg catcaccgcg
      721 aacccgctca gcgcggccgt gcacttatcg tatttcagat agtcgacttc ccatgaggcg
      781 taggttttgg cgtcactcgt ctcgtgaccg aggctgcccg agccgcctgt gtagcccccg
      841 taaatcgccg cacaggtctt ggttcctggc gtggaataca gaccaagctt gaggcctttc
      901 tcgtgtacgt aacgcaccag ggccggcatg cccgagggaa acttgctggt gttcacctga
      961 attttgccgc tcgcatcacg gccattcatc cagcagtcat cgagattgac gtactgatag
     1021 ccagcatctt tcataccgct cgacaccatg cgatcggcca caccccggat cagatcctcg
     1081 ttcaggttcg cgcagccgaa cgtattccac gagttccatc ccatgggagg agtcgaggcg
     1141 aggggagcca tgcctccggt ccccgcattg ccagccgccg cggtcgaccc accggaagtt
     1201 gaagtcgacc caccagaagc gctgctgccg cccgcgctct gcccagctaa cgagctcgag
     1261 ccaccgctcg ggggggctcc gccgagagcg ctgccgccac ctacagcggt ggctccgccc
     1321 gctgcggcac cggcaccact cgtagccatc gttccgccca tggccgagcc tccgcttgcg
     1381 gtcgtgggcc cgcccgagct ggccccgccc gctatccccc cggtgtggaa tgtgccgccg
     1441 gcgctgctcc cgcccgagac acccgctccg ccaactgcgc ccgctccgcc gggcgcgccg
     1501 cccgagccgt aggttccacc cgagcttcga ggctcgctgg tgcccgcggc taccgccggg
     1561 gtgatggggg cgtccttcgg gctactgcag cccagtatct cgagtgcgaa gatcagggtg
     1621 gcccaagagc ttctcctttc taggtgacgg tgcgcaaccg ggttgcggca ggcctgggcg
     1681 gccgatccgg aatctggagt aaggatgttt gctggtactg agagtgccga tcgcaagtcg
     1741 gtcgcgatcg ctgcttcgag ttgaatcatc atattcgtgg gaacgaccgc gtgcccgcgc
     1801 actcaccatc atcgagagag cgagcccaag ctcctcgaag ctattccggt ggcgaagcgg
     1861 tttcgtctgg cagagcgtcg tgctggaagg ctcacctctc aaagtgacaa cgcgctctca
     1921 aatctgtctt ccagaaatag tgtccctgcg acgtcagggc taggtgaccc agcgtgacta
     1981 gccacaacag cctgtctgtg gcgggccatt cgcaaccggg tggcattgtg gaatccgcga
     2041 cggcaaacgg cctcaacgcg tagttcactt gccacaggcg cgttcagcag ctctcgcgct
     2101 gtagtatttt agcgcaagga caacgcgggc cttttcggtc gaattggtaa cggatcttta
     2161 gcgggcggcc cactgcacgg atgctagcca tgaagccttt cttgctctcg cgttgcgtcc
     2221 ctggcgattt acgccagcgc atgcgcgggc gatcaggatc tgggcgcttc aggctgttct
     2281 ggggcgaggc gggaagaacg ccgcagtaca acgtaatcgt gcggtcgacg agcttgcctc
     2341 ttcgtggagt ggaccgctgg caatcagcct gtatttcgct gctcgatgat ctgaatacgg
     2401 gtgtttgccg ggcgttggtt tgcacggaac ccccgcgggc aatcgcgcaa tcggggaatc
     2461 tgcgacgcac ggggatgaag ccgtcccgcg ttcggcgcat cacatttgga cggagcccat
     2521 gacccatcga agtggcctca tattcaaagg cccgatcagc tgcatgttct tagtcattgc
     2581 gggctgcagc ggaggcggct ccagcggagt cctaacgggc gcgcaggggg gcggttcgag
     2641 cggcggcgcg gcgcagggcg ggcagagctc cggcaactcg agtggcgcgt ctcaaggcgc
     2701 tcagggtggc gtaaactcaa cgggttcagg cgggagcacg aatgcttccg gaggcagtgg
     2761 tctcactggg gggcaaacgg ctagcaccaa cggagggagc gctcaggcag gtggtcaatc
     2821 gaccgccgcg agcggtggag acgcgggaag atcgggatcc ggaggcaccc ccgccggagg
     2881 cagcagcgga aaaggcggag gaacggcttc aggaggattg gcgggtgccg cgagcggcgg
     2941 ggccggaaca atggctggcg ggcagtccgg cgagaacggc ggccaagctg ggcgaggcgg
     3001 gggagccggt caaaccggaa ctgctggtgc aagtggcgga gcggagaatc cttgcgtagg
     3061 caaggcgtgg ccctcggccg atcccactaa cgccggaccc ttcgaggtga cctccgagaa
     3121 gaacgtcgga cctctcgcgg gcgctctgcc cgatcccgtg tacgggaatg aacaacagcg
     3181 attcaatatc taccgtccaa aggacttggc gcagagcggc tactgccacc cggttctggt
     3241 ttgggcgaac ggtcacggcg acaatcccga gcagaatccg ccgaagtgca tcgtcaactc
     3301 cgccacgaat acctggtgcg gaacctatcc gatgctgatc aatcagctcg cctcgcacgg
     3361 gttcgtggtg attgcgtcac tcagcaccac gacctcgcag ggcaacccac tgccaacgat
     3421 tgccgggctg aactggttgc tgcagcaggc cgaagatccg atgagcccct actatcatcg
     3481 cctcgatacg gctcacatcg gtgcgctcgg gcactccgag ggcggcatgt cgacttgcaa
     3541 agccgccgca gatccgcgga tcaaggctat cgccacggtc tcgggaacaa cgagcctatc
     3601 ggggctgcac ggcccggcgc tgttcttctg cggcgaaaag gacgaggtcg cgaagtgctc
     3661 gggcatcaac accgtgttca agtccgtaaa agaccagccc tcgatattca tcaataacct
     3721 cacggcggac cacggtagct ggttaggtca aaatggcatc aaaggcccga cgattttcgg
     3781 cctcacggca tggtttcgga ttcacctgat gggtgacacg gagaatcgga agaggtttta
     3841 cggacagaac tgcacgttct gcacggacaa tcgcgtcaag gtcgagcaaa acagtctgat
     3901 gacgcagtga ctctgacgcg atcgtcaggg ccgcgatgag cagccatgtg gcgcgcgctg
     3961 cgccacgtgg agttactcga gcgtgagcta cgctttttcg agctgtgggc caatcaaccc
     4021 cgagtggcgc gctgcgagag gtgcgtacgt ccagggttcg ccgtggaaaa ggcgcgactg
     4081 cggggtgatg atcgcagttg cgctgccggc gtgagccgtg gtgcgctttg cgattgcggt
     4141 gctggggtgg cttccaatgg gcactccgct aaccgagcgc tctgggatgg tgtgttcccc
     4201 gactgcgcgc tttgcgagtt ttcacaattt gcattttatt cgtattcggg attgcaaaat
     4261 ccgcatcgtt cagacagtta aggtcatccg ttcgacgaac tgtccctggt gaatcaacag
     4321 caccattgtg ccgtttcgac cggttccggg gcccacgaga acaacattcg caggaacacg
     4381 atgcgtcatc aacgacagct tgcgcttgca acttggttgc tcgccttggt taccgcactg
     4441 ccgtccacgt cttacgcgga caatccgatc gtacagacga actacacggc tgaccccgcc
     4501 cccatggtgc acgaggggag gttgtacctc tacacttccc acgacgaaga caacaccgtg
     4561 agcaacttct acaccatgaa cgattggagg ctctactcca cggtcgacat ggtcaactgg
     4621 accgatcacg ggacgccagc tgccggcaag acgttcagtt ggtttacgga caacgcctgg
     4681 gcgccccagg ggatctcgcg gaacggcaag ttctatttgt acctgcccat caacaacaac
     4741 acgggtgcga agatcggcgt ggccgtcgcg aacagccccg caggcccgtt cagcgatcct
     4801 ctcggtaaag ggctcgccgc gcagggcaac ggcaatatcg accctaccgt gttcatcgac
     4861 gaggatgggc aagcctatat gtactggggc aacgcctcgc tccgttacgt gaagctgaac
     4921 tccgacatga ccagttattc gggtagcgtc aacacggtga gcctgaacgg ctttctagaa
     4981 gggccgtggt tttacaagcg agggtcgctt tactacatgg tctacgcggc caacgggacc
     5041 agcaccgaga agatcagcta cgccacgagc aatagcccca cggggccgtg gaccgtgcgt
     5101 ggggacgtca tggactcagg caagacgtac accaatcacc ctggcgtcgt cgacttcaag
     5161 ggtcactcct actttttcta tcacaactcg gccctacccg gcggcaacga cttcagacga
     5221 tcggtatgtg tcgaggaatt cacctacggc accgacggca gcattcccaa gctcaccatg
     5281 agcaccaagg gtccgaacgg catcgccacg ttgaatccct tccagcaagt ggaagcggaa
     5341 accatcgcct tctcggcggg cctcaagacc gaggtctgct ccgacacggg gggaggcatg
     5401 aacgtgagct cgatcagcaa tggagactac atcaaggtca aagatgtaga ttttgccgac
     5461 ggcgtgacct ccttcgacgc tcgcgtctcc aactctggca gcaacgcgaa gattgagctc
     5521 catctcgaca gtcaaacggg cacttcgctc ggtacctgtg acgtatcggg cgcgtcgtct
     5581 tggaccacca agacttgtat ggtgaccggc ggaagcggaa agcacgacct cttcctcaag
     5641 ttcactggcg gcagcggcga cctgttcaaa ttcaactggt ggaagttcac cggacccacc
     5701 atgccgaaca atggcaccgg cggcacggga ggcgtcggcg gtaccggcgg cgcagggcag
     5761 ggtggcgttg ccgggcaggg aagcggtggc gtcagtggag gcggtggcac ctcaacggcg
     5821 ggcggcggca atgcgtccgg cggagctggc ggcatgaccg cgagcggcgg atcgactgcc
     5881 ggaggcgcag ccggcaacgg tcaaggcgga accggcaccg ctgcatccgg agccgccaac
     5941 ggcggctcag tctctgcggg atcagggggc acacttgcga tgatgggtgg ctcgagcaat
     6001 ggcgctaagg gcggagcaac cccgtcgggt ggcactgcca gcagctccgg gggctccgcc
     6061 gggaacccgc aagtgactgg cggtctgtcc agcacgggtc cgggcgcagc tccgcccgtt
     6121 gactcctcgg gttgctcctg ccgcgtcggc gcggatcaac gccaggcggg gccgtgggcg
     6181 acgctcggat tactcggcac cgtacttctc tcccgtaaac gaagcgccgc acgcaagcga
     6241 gcgcggtcaa cttagctccg ataaggcggc tgtgcgttca gggccggaat tcgtctcatc
     6301 gggtcacttc gaatgaccag gtgagtccgg cccggagctc ttcttcgaag cgctctggcg
     6361 ctcgccctcc gcgcagggcg cagtcaatgg agagagagtc gtcgtagcgg gcgatcagac
     6421 caatcagcag gcttccggta agggctctgg agaacttgcc gtttccgaat tcccgactca
     6481 ccaggatttc tgtgactgga cgtacgcgaa ctctctctga accttccgcg atgacgtcga
     6541 gcatggcctc gtattgacgg ggcgccagga gcacgtcgtt ggctacgttc acgtggatcg
     6601 tgaattcagg ccaacgcatc gaaacaatgc tggcgacatg ggtgccgagc ttccggtccg
     6661 ggctggggag caatgctccc gcctcgatgg ctatcgaaag cccggtgcca tcctgaaggg
     6721 atccgttcct cagcatcatc ttcagcgaaa cagccgaatc ctcgagctgg ctccgtggct
     6781 cctcgctcct cttccccaaa cgaacgtcgt gtcgaccttc cagaatcgct tccaattgcg
     6841 agctgacacc gtagttcaac accacggccg gcgccgaaag tcgccaatct tgcccttggc
     6901 gaatcagcgc tgcgggcccg aattcaatct ctacctctcc cggcgacgcg atggcggcat
     6961 ccgttccggt gaacggtcgg tatgctcgcg ccgtgcccgt ccaagccaac gctcccgcgc
     7021 agcccagcgc gcagatgtca tggcgtagcc gtcgcgttct cgagagtctc atcgcggcgc
     7081 cggtgggtat gggtctcgaa agtgcagaag ggagccccgc gccgggtcgc acacccaaac
     7141 cccgcccctc tcgtcgttcg ccacacagtg agctcctcgc accgcgctca gcgtccccaa
     7201 cttcgtgagt tggccgtgcg gtgaaacctc gaatatgcat agcgtcccgt cagaggcggc
     7261 gggggcgtac agatgaccga gccgaagatt gaccgagacc agatctacgc ggggggaaca
     7321 ccgcgcagtg cccaatcgtc gtccagcgtg ctgcaagtcc agcacgccca cttggccttc
     7381 ggcgcacccg aagaacaggt agtgccgttc tgaatcgagc gcgatgccgc gagagccttg
     7441 gcagccattc tcccaggtgt cggtgattgc atacgtctgc gcgtcaagcg ctaaagtccg
     7501 gcctttccac aggtgcgagt aggcgactgc tgaatcctga tccacaacga gggattccgg
     7561 gccgccgcta acttggatag cgcgccgccg tatcagctgc ggagcactgc ccgacgacca
     7621 gtgaaagacc tcgatgctct ccgcatccgg ctcggtcacc cacacgtcct cggtctttcg
     7681 aatccatctc acatagtcgg gggagccagc gagtttggct gacgcgacga cttcgccggt
     7741 ggacggatga agagcgagta ccgctcgatt cgagcgatcc accgcgaaaa ccatgcccgc
     7801 tccaaagtcc gcggaggtca gcccgctgtc atggcctccc gaataggcgg tggcctcagc
     7861 tctgccagaa cagatgtttg aagcggcaag cgagttcggg tcaagcagcg cgacgcagtt
     7921 gctgcgcccc gccggagcaa tcgtcttgcc catctccgac gagtaaacga ggtcatcgaa
     7981 cccaagaccc tggccgagcc ctaaggggac ctcgcgggct cgattggagc tcgattctgc
     8041 gaagtcccca cgcgacacgc tcggcggtgc tggatgagcg ttgcatgtgc agcccgcgct
     8101 cgtaaagcct gcggccagaa caccgaatag gagcggaagg aacttcgctg gtctcaacgt
     8161 gcctcgagat ccccaaaacc atagtaggtg cccaatcgac gagcgcaagc caatggggct
     8221 cgcggcgtct ggttcggagg ggggcgaccg acttcgttca ggttccgttc aggttcgggt
     8281 gtatccttca cgaatgcgcg tcctcgtggt cgatgaccac ttagaaacca ggcagctcct
     8341 gctcaggaac ctgcaactcg actcgcactc ggtaaaagcc gtcgatactt gtgccgaggc
     8401 agaggttcag ctgagccgcg gtgacttcga cgtggtggtt ttggacatca tgctcccgga
     8461 cggctcgggt ctcgagcttt gctcgcgcct ccgcatgtcg caaatgaatg ctccgattct
     8521 gcttctgacg gcgcgggggg atgtgaagag ccgcgtgctc ggcctcgaat ctggcgccga
     8581 cgactacctg cccaagccgt ttgcggtttc ggagctgcgg gcgcgggtga aagcgctagg
     8641 gaggcgcggg ccgatcgcga gaggccagac tatcgcccgg ggagggctcg aggtggacct
     8701 agatgctcgc cgggtacgtg tggacgggcg cgctttgccg ctcacggcgc gagagctcgc
     8761 aatcatcgag ctgctcgcta cccggcgggg gcgcgtcgtt caacgcgatg agctcatcga
     8821 gagcgtctgg ggcgaactca cggactcagc ccgttcgagc ctggaagtac tggtcgcacg
     8881 gatccgacga aagctcggga ggcacgcgca cgcgcttcgg accgtgcgaa atctgggcta
     8941 cgtgttcgaa gtcgaggaat gaagccaacg accattgcgg ctcgcgtgag ctcggccggg
     9001 gcccgaacca cggtcacggc gctaactctg gtggccctga gtacttcatt ggccgtgact
     9061 gagctgctga ccgccaagac cgaccaactg ctcaatgcga tgggcgaacg aacggcgtac
     9121 tatctcgagg ctcgcccagc gccgatcgat tgggactgga tacgtgccga agtcgacgaa
     9181 gcacgaccaa gcgaggtgcg cgtcgaaata cgtgacccca cggggcatac gcgggctgct
     9241 tacggtccgc gaactccaat cttggcgttc caccgcaact gccgtgacga cgggaaactg
     9301 cgtgtgtgtg gtcgacgcag cgagcacttt ctagttctag ccgcgatcga tcgctcggac
     9361 gatctggctg cgcgcaatcg gctcttgttc gtgattggcg ccttgtccgc tctcgcggcg
     9421 ggtacggtcc tgctggtcag tcgctctctc atgcgggtcg ccgtgaagcc gctcactgat
     9481 cttgccgccc gagttcgagc cgtccagccc ggcagcggag aacgcgtgga gctccggagt
     9541 gacctagtgg aggtcaacga actcgccgga cgcttcgacg acttggtgga acggttcgaa
     9601 caggcgctcg cgcgcgagaa gcgctttgcg gcggtggcct cgcacgaact ccggacccca
     9661 ctcaccgtca tccaggccga aatcgaggct ttgccgaaga atgacgcgac ctcgcgcgcg
     9721 ttgctatccg tccagcggct caatcagctg gtcgaggtgc tgctttggtt cgctcgcgct
     9781 caatccgggc tgggtggtga acgcatggag tggatcaacg tcgccgacct gattcgggac
     9841 caggtttccg ctctgcaaat cgcgaacccg tcagcgcggt tttccgttca ccttccggac
     9901 gaagccttgg tgcacgggga tgagcatctg ctgggtcgtg tcacggccaa cctgctcgac
     9961 aacgccgtca aatatggcga gggctcggaa gtgtcggtca gcgctagcca cggctccgtc
    10021 gatctcgaaa tcgaggtcac gaacggcggg gctgggattc cctcggatgt gcgtgagcat
    10081 gtcttcgaac ccttctttcg cgtaccgggg cgctctgcag atggcttcgg cttgggcctg
    10141 gcgttcgcgc gagcggtggc ccgggcccat ggcggcgact tgactctctc gccgcattcc
    10201 caaagtctca ctcggttcgt gttgacgctc aggctcgcct cgtggagcga gacggaacac
    10261 ggatgattat tccgttcagt tggcgttcgg attcgcgagt tattgattct cctcgacccg
    10321 agaggaagat ccatcatgaa cgcttctgca aattcagggc gccttacgca accgtaccca
    10381 gtctttgggc tgctcttgtt actggtcagc gtgcttttga tcgcagcggc cccgccgacg
    10441 cgacccaaca tcgtggacgg catcatcggg gatcaagatc ccacacccga ggtgagtacc
    10501 gcagagttgc ggagggccgt cgccgaactt ggcgcgatcg ttctcgacgc ccgcccgtac
    10561 gaggaatacg cggtgagtca tctgcctgcg gccagaagcg taaaaggcaa agtgggaaca
    10621 acccccgcgc tgtacgtcgc cgacgtgaat gacgtgctcc aacaagtgcc ggacaagaag
    10681 cagccactca ttctgtattg caatggcctc ttctgcgggc gaagcaagcg ctttgccgcc
    10741 gaactcgttc aggcgggcta cacgaacgtt cgacgctacc aactcggcat tccggcgtgg
    10801 cgagcgctcg gaggagtcac ccaagtcgaa aaggacgctc ttatgtcgct gctcgcccgc
    10861 gatcaaacgg ccgtcttagt cgacgcccgg gagcccgggc aacggcgtcc acgtctactg
    10921 ggcgctactc ccattccgct cagcgagacc acacaggcca aagacgatgg acgcttgccg
    10981 atgacagacc acaatacacg catcttcgtc gtgggttcga gtggaaccca ggcccgcgcc
    11041 gttgccgaag ccattgtcaa agacgccttc cacaatgttg cattcttcga cgggccggag
    11101 accgaattgc cccgtttgct ggataggcat cggtgatctt tcttgggccc ggcgaggcaa
    11161 cgagcgcgac cagtcgttgc agggcgtcga aagcgtaact ctgcgcggtg agccactcga
    11221 gcccaccgcg ccggcccctc gaaataacgg cctcttcggg actctcgctg acttagctcg
    11281 cgcgctgatc aacgatcagg gcgtgggcgg agcggtcggt acgcacgcgc gggccctcgc
    11341 agggaaaaca gcagcgagtc cgcgggctgc gtggtgccgg ggtcgagtct gaggcggcga
    11401 ccgcccgtca cctcggtgaa gaacagccgc ccgttcgtca cgtgagccac gagcagcagc
    11461 ctcaacgatg gcacgcggac gatggagatt cccctggctt gcgcattgcc ggcctgagcg
    11521 gccacgacgc ccgatatgcc tcgaatgagg ttgggaaagc cgaacgaggc cgcgtgccac
    11581 tcgggcccgc ggcgcacgac actgattgac gagcggacgg ctccgtcgac gaggaccggc
    11641 accgtaaact tctcgaacgg caagacgagc gcagcaatcg aggtattggc ctcgtactgt
    11701 ttgagccgct cgagtgacac agacaggtcc cgaatcggca ccccgagttt ggcgcgctga
    11761 acgtcctctg gcttgtcgaa gcctagcccc acgcagttct gggcagtcac gagctcgcgt
    11821 aaggtggcaa ggccttcgcc agccgctttc tgagccccgg tcggtaccgg agcaagaagc
    11881 aggaggatcc ctggatccgg acgaaacttg ggatcgagcc ccggaggcac tgcgggcagc
    11941 gcgggctgcc ccaccgcgct cgacggctgc tggggccgcg ggggctgggg tagcggctgg
    12001 ggcgcgggct gcgcggccgc ggtggcgggc aaaccacaca ccaggaagaa gagggcgatg
    12061 gcggttctca tcatgagccg aaccccacat attctatatc gtagaagtcc aaactgtgag
    12121 tgtgagaccc gggctcggag gcgaaggagg cgtaatccac aatgccctca tcgccctggc
    12181 atggcgccca agggtccaga atctcgatat agtttttacc tttgacgctg gagtagccct
    12241 tggcgaccat catgtgactt cctccctcgg cccattccca ggtgaagaga aacggtgttc
    12301 gttggcaatg gtcttccctg gacaattgct ccttgacctc cgtccaactc aaaggccccg
    12361 gccattcggt cgcgtgaaat ccgtag
//
LOCUS       DASTKU010000705        20868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_356823, whole genome shotgun sequence.
ACCESSION   DASTKU010000705 DASTKU010000000
VERSION     DASTKU010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 20868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20868
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356823"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFQ61_24645"
     CDS             complement(<1..225)
                     /locus_tag="VFQ61_24645"
                     /inference="COORDINATES: protein motif:HMM:NF014930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HET9957719.1"
                     /translation="MEYEPVIGLEVHAQLLTESKLFCSCSTNFGNPPNSNVCPVCLGL
                     PGSLPVLNARAVEMAVRAALALSREIRGSSI"
     gene            complement(290..982)
                     /locus_tag="VFQ61_24650"
     CDS             complement(290..982)
                     /locus_tag="VFQ61_24650"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HET9957720.1"
                     /translation="MTDSLPTVSPAPTPLVAGPLGPRKVVGKVVDRPRRGAGVQSYVP
                     EIAKGLLITMKHFFQNTKEMAFGQRNDPSLEAIEEGVNTVSYPEQRRPYAARFRGVHR
                     LTHRQDGSPRCVACLCCSTACPAQCIHIVAGEYPPGDPRRGYERFPERFVIDELRCVF
                     CGFCVEACPCDAIRMDTGMHAAPYDSREQFLFEKDLLMSFPGRDGSFETQNPRHEPGE
                     KAHPGIDRERRH"
     gene            complement(994..2787)
                     /locus_tag="VFQ61_24655"
     CDS             complement(994..2787)
                     /locus_tag="VFQ61_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HET9957721.1"
                     /translation="MTLTPLELLISGLRAFIVVMFAMNVAVILTWADRRQGAAIQDRV
                     GPNRAVAWIPTKVAQALAVLPALGVVAGIVAFAFVAARNLDAESRPFASVVFSQLAVF
                     MWWLTGLVIAGRVVRRGPRSSFDVFVSTLGNPRNFLYGGLVAHAVTLSLGLMYRGTEL
                     GETLEQAGISIGGALLALAVAFGAGYAAYSIRNEPRIGLRLAGLLHPAADGLKTIFKE
                     DFIPPNADKLLHSIAPIIAFFPALVVLAVVPFGDSLCFGVDPETGRSAFGELFATVPA
                     TGCPGAQLPLQVVSLNVGVLYFFALAGTGIVGAALAGWASDNKFSLLGGLRAASQMVS
                     YEVTMGLTLIGAFMVYGTLRVDEMVRWQADNAWGIFLQPLAFVLFFAAAVAESKRIPF
                     DLPEGESEIVAGYYTEYAGMKFAMFFFAEYIAVVTSSALMAALFLGGWDLPFVERDGL
                     HIALGNTVFFSAPLPHVFLVVIGALGFVFKTVLLCWVQLTIRWTLPRFRYDQLMRLGW
                     RKLLPASLFNIVITGLLVLAFQSAGENVQRGLGVLADVTKLIVAVGGLVAFVSFVRFL
                     LAPRNKRRLLATTSAQFAAALGGTRSARMEA"
     gene            complement(2784..4424)
                     /locus_tag="VFQ61_24660"
     CDS             complement(2784..4424)
                     /locus_tag="VFQ61_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9957722.1"
                     /translation="MPTFKLDDREIPFEPGDTIIRAAHRVGIDIPHYCWHPGLSVAAN
                     CRMCLVEVLPPPGRPAMMLDVVAWDSDKQQYISQKKPKLQPACQMAAGDGMVVKSQTS
                     EHVIRARSAVQELLLLNHPVDCPICDQAGECRLQDYWLEHQASKKRMRDEPVHKPKGV
                     VFGPTIVYDAERCIVCTRCVRVSEELAKDPVLSVRERGNLNEIVVAPGRQLDHNYTLM
                     TEHVCPVGALTAVDFRFKARVWFLRSARSVCVGCATGCNSYTDYDPRSESVLRYRPRE
                     NLAVNKYWMCDEGMLDYRRIHKGRVLAARVQGEPTTREAALEKAAELLRVKDTSKVAV
                     VLSAEHSQEDNLAMVWLARDVLGTSRVFVTGKPPGKADNVLMHADKNPNTAGVQALAQ
                     LVPVQSFADFEAALRNNSVEAALVLGSAVPQGGALSARKLVALSSHEGKVAEKANVVL
                     PVSSWAEADGTFVNHQNLAQVSEKVIRPQGDSRPAWRVVADLAKALGRAPTWRRPEEL
                     RDALLGALTPGGAPSAGASGANSAGSSSAGSSSAGANG"
     gene            complement(4487..5701)
                     /locus_tag="VFQ61_24665"
     CDS             complement(4487..5701)
                     /locus_tag="VFQ61_24665"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET9957723.1"
                     /translation="MEPLDQALESTELDLPTEPMLLNVGPSHPAMHGTVRIVMELSGE
                     SIERCDVQIGYLHRGFEKMCERGTWTQVFPYVDRCNYVSPMLNNVAFALACEKLLGIE
                     VPERCQWYRMALGELARMSDHLTCDGAMAMELGAFTPFLWMIKGREMIWDLMEEETGA
                     RLTHSFGRVGGMAQPPTPGFKENARAVSKHILGIIEEVERLLLGNRIFLDRLEGVGVI
                     RVDEAISLGWTGPCLRAAGVPYDVRKSHPYMKYGEVDFEVPIGRNSDCFDRFIVRIEE
                     IKQSARILEQCLERLSDSGPVNVDDPRVILPPKEEVYNTIEGTIQHFKIVMEGLKLPA
                     GEVYSFTEGGNGELGFYIVSDGSGTPYRVRIRPPCYYITAGLERVITGQMMADVVPCF
                     GSLNMIGGECDR"
     gene            complement(5701..6288)
                     /locus_tag="VFQ61_24670"
     CDS             complement(5701..6288)
                     /locus_tag="VFQ61_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET9957724.1"
                     /translation="MAKLLLDLLQRQFPSAVLETSSQHGDETAVVSSAAWRAVAQFLR
                     DDPRAAMEMLSDLTVVDYPDRDPRFEVVVHLYSLSKGHRLRLKTRAGDPAGEVVRVDS
                     IADLWASANWAEREAFDMFGVEFVGHPDLRRILMYPEFVGHPLRKDYPAQQTQPLLPY
                     RDVPNIEKLPPFGPDEGMPFGRQTHAGVLTDEEAN"
     gene            complement(6440..7021)
                     /gene="nuoB"
                     /locus_tag="VFQ61_24675"
     CDS             complement(6440..7021)
                     /gene="nuoB"
                     /locus_tag="VFQ61_24675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET9957725.1"
                     /translation="MGELTHTSTRVIEPAGEQGFATTRFQDLLAWAQKYSLFMYPYVT
                     ACCGMEFMAVSSPRYDFSRFGSEAPRFSPRQSDLLWVVGTIVQRQAPVLRRIYEQMAE
                     PKWVLAFGTCASCGGFYDNYATVPGIDKIIPCDVYVPGCPPRPEAVLDGLMLLQDKIQ
                     SGDRRPAVVKPRHDPVQEPNMGLTQLRRSRTRT"
     gene            complement(7009..7368)
                     /gene="ndhC"
                     /locus_tag="VFQ61_24680"
     CDS             complement(7009..7368)
                     /gene="ndhC"
                     /locus_tag="VFQ61_24680"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET9957726.1"
                     /translation="MVDIYLPMFVMFAIVAVICSVMFLGGQLIGPKNPTPEKMQPFEC
                     GNDTQGAKGTRLSVKFYLTAILFVVFDVEVVFMYPWAALFKGLGWTGFASMFAFIVAI
                     VVALAYCWKKGALEWES"
     gene            complement(7653..8810)
                     /locus_tag="VFQ61_24685"
     CDS             complement(7653..8810)
                     /locus_tag="VFQ61_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957727.1"
                     /translation="MHAKMLGRLIVSLEAIRTDGWFERMGEGIGSFQALCDIIGARFF
                     AFAMITGARITSLTVDRRSPENTQVEFVVGGEDASLDPSDTQRLSLAEFRARLVEALV
                     TEEPLGPPPRRATDIEAVQLHIGVRYLLLAPLYGYALRELTVDEDGSEIRLLHDGVEE
                     LYPIGSFRARLRTHVREELERLGRSQSRSAIDLTRVAEAEQAASSGDHIRVLELLGAW
                     PAPLAIFLRTVEGQTLPQDTRALIARALGLLGTACVMLGESGKGEEVLRLAVQYAGDS
                     SAAADVYLRLGDAILGDARPGEAIAPLRRAANLGAPGEQVWPLLAQAFAERQRWLAAY
                     AAVLEGRAAGAPAEILEQVLEAVREGLGEQLEQLRDCVERTPPLSQTVHPG"
     gene            8889..9998
                     /gene="mutY"
                     /locus_tag="VFQ61_24690"
     CDS             8889..9998
                     /gene="mutY"
                     /locus_tag="VFQ61_24690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET9957728.1"
                     /translation="MTFEADFARLLLAWYDREHRDLPFRQTKDPYAIWVSEIMLQQTQ
                     VKSVTPYFTRWMARFPTVTSLAQASEDEVLHAFQGLGYYSRARSLHRAAQQVVRDFGG
                     RMPGTAHELRSLPGIGPYSAGAIASIAFRERVPAVDGNVVRVLTRLFAWHGDPQHNPL
                     KAQLTARAAELVPPGRPGDFNQALMELGATVCTPRAPQCAACPVARMCRAQELGLQQR
                     LPETAARPKPTDVAVAALALTRRGRVAVVKLRADAPRWAGMWQFPAMEVALPVTPAEG
                     AARVARDVLRLKVASVRELGLIRHSVTRFRISLHAFTAPTLGVGELNPEHAQEVAWKR
                     VSDLEEVAMPAAHRRIALLLLQQASGKDALPSVEP"
     gene            9995..10411
                     /locus_tag="VFQ61_24695"
     CDS             9995..10411
                     /locus_tag="VFQ61_24695"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HET9957729.1"
                     /translation="MTANPPPTIDVAIALVFRGSRVLVTRRREGTHLGGFWEFPGGKI
                     EPNETPEAAAERELMEEVELRVLPRARGSVIEHAYATRRVRLHPVYCSSEHGEARLRE
                     VAEARWVDSAELATLHFPEANRSLLAELEQRRWPPF"
     gene            10500..11306
                     /locus_tag="VFQ61_24700"
     CDS             10500..11306
                     /locus_tag="VFQ61_24700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957730.1"
                     /translation="MLAARQTAPAELPCSLDLGLSEVTASLESEGVRLPGGAFVTWAQ
                     LEHVRDHENACFELQDDELHVIRCYSEEFRRDYRLMPTTRAPALLISGFVMHRIRDTT
                     PAEGAHAMVHSLLPVRGRLLDTATGLGYAAVEAARHASEVVTIELDPSVQEVARRNPW
                     SRELFENPKITLRIGNSAELICGLAEGEFSAILHDPPAINLAGDLYSEEFYVHARRVL
                     GRAGKMFHYIGDADSASGSRTTRGVVRRLKSAGFSKVIPKPAAFGVLAIK"
     gene            complement(11312..12109)
                     /locus_tag="VFQ61_24705"
     CDS             complement(11312..12109)
                     /locus_tag="VFQ61_24705"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET9957731.1"
                     /translation="MGGLGTGELLQALPSSDRKDCESVIEAARQAANWKPPAKVPARM
                     ARAAIQKTELLVGKRLKTLGRQPALACAKGCASCCYGTKVDISAPEALLLAEFLKETL
                     GAAELAGLVDGVKQEASRIRDFSLESRALDRAPCVLLESTTQSCRVHAVRPMACRAHH
                     SLEVEPCVRAFEDPGGEHFALMDPEILRSFELGAFALEHALRAKGLDGESYELTQALL
                     IALSEPDAAQRWLKGQPIFEAARVPSDEADREWVEQERGRAPLTTAR"
     gene            complement(12135..13640)
                     /locus_tag="VFQ61_24710"
     CDS             complement(12135..13640)
                     /locus_tag="VFQ61_24710"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HET9957732.1"
                     /translation="MSHVTISELMESSGVKFGTSGARGLVEHLSARVAYAYTAGFLAH
                     LEQSGQIGAGSGLRSVAVAGDLRTSTPVILAGVCRAAQDRGFSVYHAGRVPSPAVFHY
                     AASRGIPSIMVTGSHIPEDRNGIKFNTARGEITKADEQGIRAQRVEVPDLFDARGQFK
                     DAACGQLPPVDSGAARAYVERFTKAFPAGALRGLKLAVYGHSAVGRELLVEILEALGA
                     RVSRLGWSDVFISVDTEAIRPQDVELASAWARSEGFFALLSTDGDSDRPLLADENGRF
                     LRGDVLGVLTARQLRASFVAAPVSCNSMLEQVGWFKTARTRIGSPYVIEAMEAAVRSG
                     ERAAVGFEANGGFLTASALQVPPLAGEPGGEISALPTRDAVISPLAALCAAVAQNATL
                     SALVAALPKRFTASDRLPEFPTELAQARLKAFREALPASAREFLPELGPLASKNEVDG
                     LRLSFENGEVVHLRASGNAPELRCYVEAASEARAELLLREALAKLEIWRSA"
     gene            complement(13696..16374)
                     /gene="aceE"
                     /locus_tag="VFQ61_24715"
     CDS             complement(13696..16374)
                     /gene="aceE"
                     /locus_tag="VFQ61_24715"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HET9957733.1"
                     /translation="MDSYRPDSDRDPQETQDWVESLKSVVEHTGKERARFLLTKVLET
                     ARRDRVEPVLPLNTDYVNTIGVEEEPKYPGDAALEKRIQAIIRWNAAVMVHRANHHHS
                     GLGGHISTYASSALLYEVGFNHFYRGKDAPGRGDHIFYQGHAVPGIYARSFLEGRLSQ
                     EQLEHFRQEAARGKGLSSYPHPRLMPEYWEFPTVSMGLGPIGSIYQARFNRYLQARGF
                     KDTSQQRVYCYVGDGESDEPETLGALGVAAREKLDNLCWVVNCNLQRLDGPVRGNGKI
                     IQELEAVFRGAGWHVIKVIWGPEWDSVFARDTEGVLREQLNQVVDGQWQRFTTAPGDL
                     VRREFFARDPRLLETVNHLNDDELGSMRRGGHSLRKVYAAYQRATSLLGQGKPVVILV
                     HTVKGWLLGEGFAGSNVTHQRKKFDNDELRAFRDVLGLPIDDKDLEDPPFYHPGKDSA
                     EVRYMLDRRRELGGSLPERRVKVDVPLELPKHDLYEEFYRGMDKGEASTTMVFARLLS
                     KLIRDKKIGRRIVPIVPDEARTFGMDALFGQVGIYASQGQLYEPIDKGKLLYYREAKD
                     GQVLEEGITEAGSMASFVASATSYATHGEPLIPFYIFYSMFGFQRTGDQLWLAGDQMA
                     RGFVLGATAGRTTLNGEGLQHEDGHSPLVAMTVPACRAYDVTFAFELAAVIEDGMKRM
                     YVDGENIYYYITLQNENYPMPPMPEGARDGILKGLYRFRSAEKRLDKHVQLLGSGSIM
                     RQVLAAQEILAQRFGVSSDIWSVTSYQALRSDALACERHNRLHPEDEPRVPYISRALE
                     GVSGPFITASDYMKSLGDLIGRWVPGRLVPMGTDGFGMSDTREALRRHFEVDAECIVL
                     GALDGLRQEGKLSAAELAKAIVELGVNPDKIEPTSI"
     gene            16524..17714
                     /locus_tag="VFQ61_24720"
     CDS             16524..17714
                     /locus_tag="VFQ61_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9957734.1"
                     /translation="MPRLVSFLIFFTIFLSVLGGAHYYVWARLVRDPALPAQLSRVLT
                     WAMVALFASIPATFLSGRLLGVRAGGMLAWVPFVWMGMLFLSLVVVAGLDLVRAGWLL
                     VRAAGDASPIDPERRLWFSRAFAGAAGLLVSGLGLAAVRSGLSRVLAREVRVDLERLP
                     SELDGVTIAQLTDVHVGPTIQRSFVEQIVESTNALNPDVIAITGDLVDGSVEELRDHV
                     APLARLRARYGVYFVTGNHEYYSGVEEWCRELTRLGIRVLRNERVTIGVGEASFDLAG
                     VDDYQARSFGEDHGPNLGRATAGRDPSRELVLLAHQPRAVFEAQHHGVGLQLSGHTHG
                     GQLFPWNFMVRLQQPVVSGLARFGRTLLYVSNGTGYWGPPMRLGAPAEITRLVLRAPA
                     EAVA"
     gene            17996..19360
                     /locus_tag="VFQ61_24725"
     CDS             17996..19360
                     /locus_tag="VFQ61_24725"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9957735.1"
                     /translation="MLLRLSFWAAGTLTLAAACSFEDPKSDDGGSGGGGAGGQTAVGG
                     QTTASGGKASGGTTSKPTGGTSNQGGTTTAAGTSGEAGAGGQAGETSSASGGTSGAEG
                     GAGGEEAGGAPEGGAGGSEPGGGAGAGGDTTEPVVIGSSIYPKPPGEDTVPQPAGTVG
                     GLTVLNWAGFKSAVSYTFDDSNETQIKHYDDLKSLGVRYTFYLQTGKGAFSDPTWARA
                     AQDGHELGNHTVHHLNQADLSLLPMPTDEVDLATKAIETSYGVKVFTMAGPYGASAYE
                     VMAQNRFFINRGASDGMMLASSPGSAFNVACFIPPDGATVDTFNTQVNQTRTASAWKV
                     FLVHGFKDAVVKDWAYLPVDYPVFQDAVNYAKGLGDVWIDSVKNVGAYWLGQRAFNAA
                     TKSVSGSSTTWTWQLPAHFPPGNYLRVTVTGGTLKQQNTPLAWNPRGFYEVALDAKSL
                     TLSP"
     gene            complement(19366..>20868)
                     /locus_tag="VFQ61_24730"
     CDS             complement(19366..>20868)
                     /locus_tag="VFQ61_24730"
                     /inference="COORDINATES: protein motif:HMM:NF044849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9957736.1"
                     /translation="LEDLPEIVLRKRSLDARRGAVRFHLLFGFQTVSGQPGFPALRLP
                     RPVRGDPEVVIVGDGPAGLFCAYELACHGIAAIVVDRGKTVQPRRHDLKALHRGGVVN
                     EDSNYCFGEGGAGTYSDGKLYTRSHKRGNVRDVLEILALAGAPAEILTEARPHIGSNR
                     LPKVVTALRERIEAAGSQFRFGARVVDILVEHGPQGARVCGVALAGGEQILARHVVLA
                     TGHSARDILEITQRLGLSLEPKAFALGVRIEHPQPLINRIQYGASADHPKLPNAAYRV
                     AHEVEGRGVFSFCMCPGGWIVPASTEPGGLVVNGMSLARRDSPFANSGFVVSVEPSDW
                     ERAGFTGALGGVLLQRKLEQIAFDAGGGELRAPATRATDFVRRQASSTLPDTSYIPGL
                     TPTNLWDVFEPLGLGIAPRLSAALQTFGRRMPGYVSEDVVLVGVESRTSCPVRLVRDG
                     QTLQARGLAGLYPTGEGAGYAGGIVSAAIDGVRVAQAVCVELGVEPTASE"
BASE COUNT         3795 a         6946 c         6531 g         3596 t
ORIGIN      
        1 gatgctgctg ccgcggatct cgcgggacag cgcgagggcc gcgcgcacgg ccatctcgac
       61 cgcgcgcgcg ttcaacaccg ggagcgatcc cggcagcccc aaacaaaccg ggcaaacgtt
      121 ggaattcggc ggattaccga agttcgtcga gcacgagcag aacaacttgc tctcggtgag
      181 cagctgggcg tgaacctcaa ggcctatgac tggctcgtac tccatgaacc tcgtactccg
      241 ggaaactggg tgctccgttg gcaacgctga cggctagttt gcttccgaat cagtgacggc
      301 gctcgcgatc gatccccggg tgcgccttct ccccgggctc atggcgcggg ttctgggtct
      361 cgaagctgcc gtcacggcca gggaaggaca tcaggaggtc cttctcgaac aggaactgct
      421 cgcgcgagtc gtaaggggcg gcatgcatgc cggtgtccat gcggatggcg tcgcaggggc
      481 aggcctccac gcaaaaaccg caaaacacgc agcgtagctc gtcgatcacg aagcgctccg
      541 ggaagcgctc gtatccgcgc cgcgggtctc cgggcgggta ctcgccggcg acgatgtgaa
      601 tgcactgcgc cggacacgcg gtggagcagc acaaacacgc gacgcagcgt ggcgagccgt
      661 cttggcggtg agtgagccgg tgcacgccgc ggaagcgcgc ggcgtagggc cggcgctgtt
      721 ccgggtagct caccgtgttc acgccttcct cgatggcttc caagctcgga tcgttgcgct
      781 gcccgaacgc catctccttc gtattctgga agaagtgctt catcgtgatg agcaggccct
      841 tggcgatctc gggcacgtag ctctggacgc ccgcgccgcg tcgcggccgg tcgacgactt
      901 tgccaacgac cttgcgcggg ccgagcggcc ccgcgacgag gggcgtgggc gcaggcgata
      961 cggtggggag tgaatccgtc atgactccga tcctcatgct tccatacggg ccgagcgagt
     1021 gccgcccaaa gccgccgcga actgcgcgga tgtagtggcg agcaagcgcc gcttgttacg
     1081 cggagccagc aagaagcgca cgaaacttac gaacgcgacc aaaccaccga cggccacgat
     1141 caacttggtg acgtcggcca acacgcccag gccgcgctgc acgttttcgc ccgcggattg
     1201 gaacgcgagc accaatagcc cggtgatcac gatgttgaag agagacgccg gcagcaactt
     1261 gcgccagccc agccgcatca actggtcata acggaagcgc ggcaaggtcc agcgaatggt
     1321 gagctgcacc cagcacagca gcaccgtctt gaatacaaag ccgagcgccc cgattacgac
     1381 gaggaacacg tgaggcagcg gggcgctaaa gaacaccgtg ttgcccaacg cgatgtgcag
     1441 accatcccgc tccacgaacg gcaagtccca accgccgagg aacagggccg ccatcagcgc
     1501 gctcgaggtc acgaccgcga tgtactctgc gaagaagaac atcgcgaact tcatgcccgc
     1561 gtattccgtg tagtaacccg ccacgatctc gctctcgccc tcgggcaagt cgaacgggat
     1621 gcgcttggac tccgccaccg ccgccgcgaa gaacagcacg aacgcgagcg gctgcaagaa
     1681 gatgccccag gcgttgtcgg cttgccagcg caccatctcg tcgacgcgca gggtgccgta
     1741 caccatgaac gcgccgatca gcgtgagccc catggtcacc tcgtaggaga ccatttggct
     1801 tgccgcgcgt agcccaccga gcaagctgaa cttgttgtcg ctcgcccagc ccgccagcgc
     1861 ggcgccgacg atacccgtgc cggccagagc gaagaagtag agtacgccga cgttgaggct
     1921 aaccacttgc aacggcagtt gcgcaccggg gcagcccgtc gccggaaccg tcgcgaacaa
     1981 ctcgccgaac gcggagcgac ccgtctcggg atctactccg aaacacaggc tgtctccgaa
     2041 cggcaccacg gccagcacga ccagggccgg aaagaaggca atgatcgggg cgatactgtg
     2101 caggagcttg tcggcgttcg gaggaatgaa atcctctttg aagatcgtct tcaagccgtc
     2161 tgcggccggg tgcagcaacc ccgccagtcg cagcccgata cggggctcgt tgcggatgct
     2221 gtatgccgcg tagccggcgc cgaatgcgac cgcgagcgcg agcagagcac cgccgatcga
     2281 aatgccagct tgctcgagcg tctcaccgag ttccgttcct cggtacatca gacccaagct
     2341 caacgtcacg gcatgagcga cgagcccgcc atacaagaaa ttgcgcgggt taccgagcgt
     2401 ggaaacgaat acatcgaagc tgctgcgggg gccccggcgc accacacggc cggcgatcac
     2461 gagcccagtc agccaccaca tgaacaccgc gagctgactg aacaccacgc tcgcgaatgg
     2521 gcgtgactcg gcgtccagat tacgcgcggc aacgaacgcg aacgccacga tgccggctac
     2581 gaccccgagc gcgggcagca ccgcaagcgc ctgcgccacc ttcgttggga tccacgcgac
     2641 cgcgcggttc gggccgacgc ggtcctggat ggccgcaccc tgacgacggt ctgcccaggt
     2701 cagaatcacc gccacgttca tcgcgaacat cacgacgatg aacgcgcgca gacccgaaat
     2761 taagagttcc aaaggagtga gcgtcatccg tttgctcctg cactgctcga acctgcactg
     2821 ctcgaacctg cactgttcgc gccgcttgca ccggcgctgg gcgcgccacc cggagtcagg
     2881 gcgcccaaca gggcgtcgcg tagttcttcg gggcggcgcc aggtcggcgc gcggcccagc
     2941 gctttcgcga gatcggccac cacgcgccaa gcgggacgtg aatccccctg gggccggatg
     3001 accttttcgc tcacctgggc gaggttctga tgattgacga aagtgccgtc cgcttcggcc
     3061 cagctcgaga cgggcagcac cacgttggcc ttctctgcga ccttgccctc gtggctggag
     3121 agcgcgacca acttgcgcgc gctcagcgcg ccgccctgcg gaaccgcaga accgagcacc
     3181 aacgctgctt cgaccgagtt gttacgcagc gcggcctcga agtccgcgaa cgactggacc
     3241 gggaccagct gcgccaacgc ctgcacgccc gcggtgttcg ggttcttgtc cgcgtgcatc
     3301 agcacattgt cggctttccc cggcggcttg ccggtcacga acacacgcga tgttccgagc
     3361 acgtcacggg ccagccagac catggccaaa ttgtcttctt gcgagtgctc ggcgctgagc
     3421 acgacggcca ccttgctcgt gtctttgacc cgcaatagct ccgccgcctt ttcgagcgcg
     3481 gcctcgcgcg tcgtgggctc accctgcacc cgcgcggcca acacccgacc cttgtggatg
     3541 cggcgatagt cgagcatgcc ctcgtcgcac atccagtact tgttgacggc caggttctcg
     3601 cgcggacggt aacgaagtac cgactcggaa cgcggatcgt agtccgtgta cgagttgcag
     3661 cccgtggcac agccgacgca aaccgaccgt gccgagcgca agaaccagac ccgcgccttg
     3721 aaccggaaat ccacggccgt gagcgcaccg accgggcaca cgtgctcggt catcagggtg
     3781 tagttgtgat cgagctggcg ccccggggcg accacgatct cgttcaggtt gccgcgctca
     3841 cgcacggaca gcaccggatc tttcgccagc tcctcggaga cgcgcacgca gcgcgtacac
     3901 acgatgcagc gctcggcgtc gtacacgatg gtgggtccga acacgacgcc cttcggcttg
     3961 tgcacgggtt catcgcgcat gcgcttcttc gacgcttggt gctcgagcca gtagtcctgc
     4021 agccggcact cgcccgcttg atcgcagatt gggcagtcta ccgggtgatt cagcagcagc
     4081 agctcctgaa ccgccgatcg cgcccggatg acgtgctcgc tggtctggct cttcaccacc
     4141 atgccgtcac ccgccgccat ctggcaggcc ggctgcagct tcggcttctt ttgcgagatg
     4201 tactgctgct tatccgaatc ccaagccacc acgtccagca tcatcgcggg cctgccgggc
     4261 ggcggcagta cctcgaccaa gcacatgcgg cagttggccg cgacgctcaa ccctgggtgc
     4321 cagcagtaat gcggaatatc gatgcccacg cgatgggcag cccgaatgat cgtgtcaccg
     4381 ggctcgaagg ggatctcacg gtcgtccagc ttaaacgtag gcatcaagac tccgaagcgc
     4441 gtctttaagg cagattctta gagcacacac agcagcatgc cgagattcag cgatcgcact
     4501 cgccgccgat catgttgagg gagccgaagc acggcaccac gtccgccatc atctggccgg
     4561 tgatcacgcg ctcgaggccc gccgtgatgt agtagcaagg cggacggatc cgaacgcggt
     4621 aaggcgtacc gctgccgtcg ctcacgatgt agaagccgag ctcgccgttg ccaccttccg
     4681 tgaacgagta aacctcgccc gcgggcagct tcaggccttc catcacgatc ttgaagtgct
     4741 gaatcgtgcc ttcgatcgtg ttgtagacct cttccttggg agggaggatg acgcgcggat
     4801 catcgacgtt caccgggccc gaatcggaca agcgctcgag gcactgctcg aggatgcgtg
     4861 cagactgctt gatttcctcg atacgtacga tgaagcgatc gaagcagtcg ctgttgcgac
     4921 caatcggcac ttcgaagtcc acctcgccat acttcatgta agggtggctc ttacgcacgt
     4981 cgtacggcac gcctgcggcg cgcagacacg ggcccgtcca gccgagcgaa attgcttcgt
     5041 cgacgcgaat gacgcccaca ccctcgagcc gatccaggaa gatgcggtta ccgagtagca
     5101 ggcgctcgac ctcttcgatg atgccgagaa tgtgcttcga gaccgcccgc gcgttctcct
     5161 tgaaaccggg cgtcggaggc tgggccatcc cgcccacacg gccgaagctg tgggtcaaac
     5221 gggcgcccgt ctcctcttcc atcaagtccc aaatcatctc gcgccccttg atcatccaaa
     5281 ggaacggagt gaaagcgccg agctccatcg ccatggcgcc gtcgcacgtc aggtggtcgc
     5341 tcatccgcgc gagctcaccg agtgccatgc ggtaccactg gcaacgttcg ggcacttcga
     5401 tgccgagcag cttctcgcat gccaaggcga acgccacgtt gttgagcatc ggcgagacgt
     5461 agttgcagcg atcgacgtac gggaacacct gcgtccaggt gccgcgttca cacatcttct
     5521 cgaagccgcg gtgcagataa ccaatctgaa cgtcacagcg ttcgatcgac tcgccggaga
     5581 gctccatcac gatgcgcacc gtgccgtgca tcgcaggatg cgaaggcccc acgttgagca
     5641 acatgggctc ggtcggcaga tccagctcgg tggactcgag cgcttgatcc aagggttcca
     5701 tcagttcgcc tcctcgtcgg tgagcacgcc ggcgtgagtc tggcggccga agggcatgcc
     5761 ttcatccggt ccgaatgggg gtagcttttc gatgttgggc acgtcccgat agggcagcag
     5821 cggctgcgtt tgctgcgcgg ggtaatcttt gcgcagcgga tgccccacga actcggggta
     5881 catcaagatg cgtcgcagat ccgggtggcc gacgaactcg accccgaaca tgtcgaaggc
     5941 ctcgcgctcg gcccagttcg cgctggccca gagatccgcg attgaatcga cccggacgac
     6001 ctcgccggct gggtcgcccg cacgcgtctt caaacgcagg cggtgccctt tcgagagcga
     6061 gtacagatga acgacgacct cgaagcgcgg atcgcggtcc gggtagtcga ccaccgtcag
     6121 atccgacagc atctccatgg ctgcgcgcgg gtcgtcgcgc aggaactgag cgacggcgcg
     6181 ccaagctgca gaggacacga ccgccgtctc gtccccgtgc tgggagctgg tctcgagcac
     6241 cgcgctcgga aactgacgtt gaagcaggtc aagcaggagt tttgccactg tatgttccga
     6301 atgtttggac gttcggctgt tccgaggttg ggtcgctcga ggttcgctcg ctcaagaggt
     6361 gaccttggac tcggattcga gctcgagtct gcaaagtcac gttccagtcc ttccggtcgc
     6421 cgctgctacg cctctagggt taggtgcgcg tgcgcgaacg gcgcagctgc gtcaagccca
     6481 tgttcggttc ttgaacggga tcgtggcgtg gcttgaccac ggccggacgg cgatcgccgc
     6541 tctgaatctt gtcctgcagc agcatcaaac catcgagcac ggcctcggga cgtggcgggc
     6601 aaccggggac gtacacgtca cacgggatga tcttgtcgat gccggggacg gtcgcgtagt
     6661 tgtcgtagaa gccgccgcag gatgcgcagg tgccgaacgc cagcacccac ttcggctccg
     6721 ccatctgctc gtaaatgcgg cgcagaaccg gcgcctggcg ctgcacgatc gtgcccacga
     6781 cccagagcaa atcggattgg cgcggtgaga agcgcggggc ttccgagcca aagcgcgaga
     6841 agtcgtagcg cgggctcgag acagccatga actccatgcc gcaacaagcc gtcacgtacg
     6901 gatacatgaa cagcgagtac ttctgcgccc acgcaagcaa atcctgaaag cgcgtcgtgg
     6961 cgaagccctg ctcacccgcg ggctcgatca cacgtgtcga ggtatgtgtc agctctccca
     7021 ttctagagct cccttcttcc agcagtaggc cagcgcaacc acgatcgcga cgatgaacgc
     7081 gaacatgctc gcgaagccgg tccaacccag gcctttgaac aacgccgccc aggggtacat
     7141 gaacacgacc tcgacgtcga aaacgacgaa cagaatcgcg gtcaggtaaa acttcacgct
     7201 caagcgtgtg cccttcgcgc cttgagtgtc gttgccgcac tcgaaaggct gcatcttctc
     7261 gggcgtcgga tttttgggtc caatcagttg tccgccgagg aacatcacgc tgcagatcac
     7321 agcgacgatg gcgaacatga cgaacatggg gaggtagata tccaccatga aacagaaacc
     7381 ttaggggtac gtggtcgccc gaaatggaaa gcgacgcggg gatctactgc ctgcccaagc
     7441 aggctgtcaa tgcgagcagc gagctttgag ccgctccgcg ctcgcccccg ggcgcaggct
     7501 tcgaaggata tgtccggtcg agcgccgctg ccatggctgc ccgtactcgt aacatgcgag
     7561 cccgtgccgc gagccaaccg gcggcccggc gccagattca gagcgatttt cgctagtccg
     7621 ccgaggctcg gctacttgtg cagtgcttgg actcagccgg gatgcaccgt ttgagacaaa
     7681 ggtggcgtgc gttcgacaca atccctgagc tgctcgagct gttcgccaag cccctctcgc
     7741 accgcctcga gaacctgctc caagatctcg gccggcgcac cggccgcgcg cccctcgagg
     7801 accgctgcat acgctgcgag ccaacgctgc cgctcggcga acgcttgcgc caacagcggc
     7861 caaacctgtt ctccgggggc acccaggttg gccgcccgcc gaagcggggc gatcgcctca
     7921 cctggccgtg catcacccaa aatggcgtcc ccgagccgta ggtaaacgtc ggccgcggcg
     7981 ctgctgtctc cggcgtattg cacggctagg cgcaagacct cttcgccttt cccgctctcg
     8041 cccagcatca cgcacgcggt gccgagcagc ccgagcgccc gcgcgatcaa agcgcgagta
     8101 tcttggggca gggtttggcc ctccaccgtg cgcaagaaga tcgcgagcgg ggcgggccac
     8161 gcgccgagca gctccaacac gcgaatgtgg tcgcccgagc tcgcggcctg ctccgcttct
     8221 gcgacgcgcg tcaggtcgat tgcgctgcga gactgcgacc gcccgagccg ttctagctcc
     8281 tcacgcacgt gcgtgcgaag ccgcgctcga aagctaccga tcggatagag ctcctccacc
     8341 ccgtcgtgca gcagacggat ctccgatccg tcttcgtcta ccgtgagctc gcgcagcgca
     8401 tagccataga gcggagcgag taacagatag cgaacgccaa tgtgcagctg cacggcctcg
     8461 atgtccgtcg cgcggcgcgg cggcgggccg agtggctctt ccgtgacgag cgcctccacc
     8521 agccgcgccc gaaactccgc gagcgagagc cgttgggtat cgctcgggtc gaggctcgcg
     8581 tcctcgccgc ccaccacgaa ctccacttgc gtgttttcgg ggcttcgccg atcgacagtc
     8641 agtgacgtga tgcgggcacc ggtgatcatc gcaaacgcga agaagcgcgc gccgatgatg
     8701 tcacatagcg cttggaagct gccgatgccc tcccccatac gctcgaacca tccgtccgtg
     8761 cggattgctt cgagcgagac aatcaaacga ccgagcattt tggcgtgcat tttcacacgc
     8821 tttacgcgcc agccacaagg agcttctcct ttatccgcgc tcgagtatgg tggcggcgag
     8881 cacccccttt gaccttcgag gcagacttcg cgcggctttt gttggcctgg tacgaccgag
     8941 aacaccgcga cctacctttc cgccaaacca aggacccgta tgcgatttgg gtcagcgaga
     9001 tcatgctcca gcagacccag gtcaagagcg tcactcccta cttcacgcgc tggatggcca
     9061 ggtttccgac ggtgacgagc ctggcccagg cgagtgagga tgaggtgcta catgccttcc
     9121 aggggctcgg ttactactcg cgtgcgcgga gcttgcatcg ggcggcgcag caggtggtga
     9181 gggacttcgg ggggcgcatg ccaggcacag cccacgagct caggagcctg ccgggcatcg
     9241 gcccctactc ggcgggggcc attgcgagca ttgcgttccg tgagcgcgtc cccgccgtgg
     9301 acggcaacgt ggtgcgcgtg ctcacccgcc tttttgcgtg gcacggtgac ccgcaacaca
     9361 atccgctgaa ggctcagctc acggcgcggg ccgctgagct cgtgccgcct gggcgaccgg
     9421 gggatttcaa ccaggcgctg atggagcttg gcgccaccgt gtgcactcct cgcgccccgc
     9481 aatgcgccgc ctgccccgtt gcgcggatgt gccgcgcgca ggaactcggg ctgcaacagc
     9541 gcttgcccga aaccgcggct cgacccaagc ccaccgacgt ggccgttgcg gcgctcgcgc
     9601 tcacgagacg cggccgagtc gcagtcgtaa agctgcgggc agacgcccca cgctgggcgg
     9661 gcatgtggca gttcccagcg atggaagtcg cactcccggt cactccggcg gagggcgcag
     9721 cgcgcgttgc gcgcgacgtc ctccgcttga aggtcgcaag cgtccgcgag ctcggcttga
     9781 tccgacatag cgtgacccgc tttcgcattt cgctccacgc tttcacggcg ccaacactcg
     9841 gcgtcggaga gctgaacccc gagcatgccc aggaagtggc ctggaagcgc gtgagcgatt
     9901 tggaggaggt tgccatgcca gccgcgcacc gccgaatcgc tctactgctg ttgcagcagg
     9961 cctcgggcaa agacgcgctc ccgagcgtcg aaccatgacc gctaacccgc cgcccacgat
    10021 cgatgtggcg atcgcgctgg tatttcgcgg ctcgcgcgtg ctggtgaccc gacgccggga
    10081 aggcacgcac ctcgggggct tctgggagtt ccccggcggt aagatcgaac ccaatgaaac
    10141 tccggaagcc gcggcggagc gcgagctgat ggaagaggtg gaactacggg tcctgccccg
    10201 cgcccgaggt tccgtcatcg agcacgccta tgcgacgcgc cgtgtgcggc tgcacccggt
    10261 ttactgcagc tccgagcacg gggaggcgcg tttacgggag gtagccgagg cgcgctgggt
    10321 cgattcggct gagctcgcga cgctccattt tcccgaagct aaccggagcc tgctggctga
    10381 gctcgagcag cggcgctggc ccccctttta gtcgagcttg ggcaaagcca tcaggctcct
    10441 ccaacccgga ccgagatccc ctgaacccac ctgtactctc tcaccttcaa gcacgacctt
    10501 tgctcgcggc acggcaaacc gcgccggcag agcttccctg ctctttggac ttgggcttga
    10561 gcgaggtgac ggcttcgctc gaaagcgagg gagttcgttt gccaggaggg gcgttcgtta
    10621 cgtgggcgca actcgagcac gtgcgagacc acgagaacgc ttgcttcgag ctccaggatg
    10681 acgaactcca cgtcatccgc tgctactcgg aggagtttcg ccgagactac cgcttgatgc
    10741 cgaccacgcg cgcgcccgcg ctgctgattt caggcttcgt catgcaccgc atccgcgaca
    10801 ccacccctgc ggagggcgcg cacgcgatgg tgcacagctt gctcccggtt cgcggccgac
    10861 tgctggacac ggcgacgggc ttgggctacg cggccgtgga agccgcacga catgcgagcg
    10921 aggtggtcac gatcgagctc gatccgagcg tgcaggaagt agcgcggcgg aacccatggt
    10981 cacgcgagct gttcgaaaac cccaagatca ccctgcgcat cggcaacagc gccgagctga
    11041 tttgcggcct agccgaaggt gaattcagcg ccatcctgca cgatccgccc gccataaacc
    11101 tcgcgggcga cctatattcg gaggagttct acgtccacgc gcggcgagtg ctcgggcgcg
    11161 cgggcaagat gttccactac attggggatg cggacagcgc atccggttcg agaacgacgc
    11221 gcggggtcgt ccgccgtctc aagagcgcgg gcttttcgaa ggtgatcccc aagcccgccg
    11281 cctttggcgt actagcgatc aagtagcggc gtcatcgcgc cgtcgtgagt ggcgcgcgac
    11341 ctcgctcttg ctcgacccac tcgcggtcgg cctcgtcact cgggacccgc gccgcctcga
    11401 atatcggctg ccctttcagc cagcgctgcg ctgcatcggg ctcactcaac gcaatcagga
    11461 gagcttgggt cagctcatag ctctcgccat ctagcccttt cgcgcgcagc gcgtgctcga
    11521 gcgcgaacgc gccgagctcg aagctgcgca agatctcggg gtccatcagc gcgaagtgct
    11581 cgccccccgg atcttcgaaa gcccgcacgc agggctcgac ttccaaggaa tggtgagccc
    11641 gacatgccat cggccgcacc gcgtgcaccc ggcacgactg cgtcgtcgat tcgagcaaga
    11701 cgcaaggagc gcggtccagt gcccggctct cgagcgaaaa atcgcggatc cgcgaggcct
    11761 cttgcttcac cccatcgacc aaaccggcga gctcggccgc gcccagcgtc tctttcaaaa
    11821 actccgcaag caaaagggct tccggcgcgg aaatatcgac cttcgtaccg taacagcacg
    11881 aggcacagcc cttcgcacaa gccagagccg gctgccgtcc caaagtcttc aaacgcttcc
    11941 cgaccagcag ctccgtcttc tgaatcgcag ccctggccat gcgcgcggga accttggccg
    12001 gcggcttcca attggcagcc tgccgcgccg cttcaatcac agattcgcag tctttccgat
    12061 cgctcgacgg gagcgcctgc agcagttcac cggtacccag tccgcccatt tgaccggtcg
    12121 tcgataggag acagctaggc ggatcgccag atctccagct tggcgagcgc ctcacgcaac
    12181 aagagttcgg cgcgagcttc gctcgccgcc tccacgtagc agcgcaactc tggcgcgttc
    12241 ccggaggcgc gcagatgcac cacctctccg ttctcgaagc tcaagcgcaa tccgtcgact
    12301 tcgttcttcg atgcgagcgg tccgagctcg ggtaagaact cgcgggcact cgccggcaaa
    12361 gcttcccgga atgccttgag tcgcgcttgc gcgagttcag tcgggaactc cggcagccgg
    12421 tcactggcgg tgaagcgctt cggcagcgcg gccacgagcg ctgagagagt cgcgttttgc
    12481 gccaccgccg cacacaacgc ggcgagcggc gagatcaccg cgtcgcgcgt gggtaacgcg
    12541 ctgatttcgc cgccgggttc acccgcgagc ggagggacct gcagcgcgct cgcagtcaag
    12601 aagccgccgt tcgcttcgaa gccgaccgcc gcacgctcac ccgatcgtac cgccgcctcc
    12661 atggcttcga tgacgtaagg agatccgatg cgggtgcgcg cggttttgaa ccaacccacc
    12721 tgctcgagca tgctgttgca gctcacgggc gcggccacga agctcgccct cagctgccga
    12781 gcggtgagca cgccgagcac gtccccgcgc agaaagcgtc cgttttcatc ggcgagcagc
    12841 gggcggtcac tgtccccgtc cgtcgagagt aatgcgaaga agccttcact tcgcgcccag
    12901 gccgaagcga gctcgacgtc ttgcgggcga atggcctcgg tgtcgaccga gatgaagacg
    12961 tccgaccaac ccaaccggct aacgcgcgcg ccgagcgctt ccaaaatctc cacgagcagc
    13021 tcgcgaccca ccgcggagtg cccgtaaacg gcgagcttca gcccacgcaa ggcgcccgcc
    13081 ggaaacgcct tcgtgaaacg ctccacgtaa gcgcgtgccg cccccgaatc caccggcggt
    13141 aactgtccgc aggcggcgtc tttgaattgt ccgcgcgcgt cgaacaggtc cggcacctcc
    13201 accctttggg cgcgaatgcc ctgttcgtcg gccttcgtga tttcgccgcg cgcggtgttg
    13261 aacttgatgc cgttgcgatc ctccgggata tggcttcccg tcaccatgat cgaaggaatc
    13321 ccgcgtgagg cggcgtaatg aaacaccgcc gggctcggaa cacgcccggc gtggtagacg
    13381 ctgaaacccc ggtcctgcgc ggcccgacac acgccggcca gaatgaccgg cgtgctcgtg
    13441 cgcagatcgc ccgccaccgc gacgcttcgc aggccagacc ccgccccgat ttgcccgctc
    13501 tgctcgagat gagcgagaaa ccccgccgtg taagcgtagg cgacccgcgc gctgaggtgt
    13561 tcgacgaggc cgcgggcccc gctcgtgccg aacttcacgc cggagctttc catcaattct
    13621 gagatcgtga cgtgcgacac gggacggggt cctcctatcg attcgagagt aacggcgact
    13681 cggggttgac cagcatcaga tgctggttgg ctcgatcttg tctgggttca caccgagctc
    13741 gacgatggcc ttggccagct cggctgcgct gagcttccct tcttgacgaa gcccatccag
    13801 agcgccgagc acgatgcatt ctgcgtccac ctcgaaatgt cgacgcaagg cttcgcgtgt
    13861 atcgctcatg ccgaagccgt ccgtgcccat cggcactaga cgccccggca cccaacggcc
    13921 aatcaagtca ccgagcgact tcatgtagtc ggaggccgtg atgaacggac cgctgacgcc
    13981 ttcgagcgcg cgactgatgt acggaacgcg cggctcgtcc tcgggatgca agcggttgtg
    14041 acgctcgcag gcgagcgcgt ccgatcgcaa agcttggtag ctcgtgacgc tccaaatatc
    14101 ggatgaaacg ccgaagcgtt gcgccaggat ttcctgtgcc gctagcactt ggcgcatgat
    14161 cgatccgctc ccgagcagtt gcacgtgctt atcgagccgc ttctctgcgc tgcggaaccg
    14221 atagagccct ttcaggatgc cgtcgcgggc tccttccggc atcggcggca ttgggtaatt
    14281 ctcgttctgc agcgtgatgt aatagtagat attctcgccg tcgacgtaca tgcgcttcat
    14341 gccgtcttcg atcacggccg cgagctcgaa cgcgaaggtc acgtcgtaag cgcggcaagc
    14401 cggcaccgtc atcgcaacga gtgggctgtg tccgtcctcg tgttggaggc cttcaccgtt
    14461 gagcgtggtg cgaccggcgg tagcaccgag cacgaacccg cgcgccatct gatccccggc
    14521 caaccaaagc tggtcacccg tgcgctggaa gccgaacatc gaatagaaga tgtagaacgg
    14581 aatcagcggc tcgccgtgcg tcgcatacga ggttgccgac gccacgaacg aggccatgga
    14641 accggcttcg gtgatgccct cttcgagcac ttggccgtct ttcgcctcgc gataatagag
    14701 cagcttgccc ttgtcgatcg gctcgtacag ctgcccctgc gaagcataga tgccgacctg
    14761 accgaatagc gcatccatgc cgaacgtacg ggcctcgtcc ggaacgatcg gcacgatgcg
    14821 gcgaccgatc ttcttgtcgc ggattagctt cgagagcagg cgcgcgaaca ccatcgtggt
    14881 cgaggcctcg cccttgtcca tgccgcgata gaactcttcg tacagatcgt gcttcggcag
    14941 ctcgagcggc acgtccacct tcacgcggcg ttctgggagt gaaccgccga gctcacgtcg
    15001 gcgatccaac atgtagcgga cctccgcact gtccttgccg gggtgataga agggcggatc
    15061 ctctagatcc ttgtcgtcga tcggtagccc gagcacgtct cggaacgcgc gcagctcgtc
    15121 gttgtcgaac ttcttgcgtt ggtgggtgac gttgcttccc gcaaaacctt ccccgagtag
    15181 ccaacccttc accgtgtgca ccaggattac gaccggcttg ccctggccga gcaagctcgt
    15241 cgcgcgttga taagccgcgt acaccttgcg caggctgtgc ccaccgcgac gcatcgaacc
    15301 gagttcatcg tcgttgaggt ggtttaccgt ctcgagcaag cgcgggtctc gcgcgaagaa
    15361 ctcgcgccga accaaatccc ccggcgcggt ggtgaaacgc tgccactggc catccaccac
    15421 ctgattcagc tgctcgcgca gcacgccttc cgtgtccctc gcgaacaccg agtcccactc
    15481 cgggccccag atcaccttga tcacgtgcca gcccgcgccg cggaacaccg cctcgagctc
    15541 ttgaatgatc ttaccgttgc cgcgcaccgg accgtcgagg cgctgcaagt tacagttcac
    15601 gacccagcac aggttgtcga gcttctcgcg cgccgcgacg ccgagcgctc cgagcgtttc
    15661 cggctcatcg ctctcgccat cgcccacgta gcagtaaacg cgttgttgcg acgtatcctt
    15721 gaagccacgc gcttgcaggt agcgattgaa ccgcgcctgg tagatcgatc caatcggacc
    15781 gaggcccatg ctcacggtcg gaaactccca gtactcgggc atcaagcgcg ggtgcggata
    15841 ggacgagagc cccttgccgc gcgcggcctc ctgccggaag tgctccagct gttcctggct
    15901 caaacggccc tcgaggaacg aacgagcgta aatgccgggc acggcgtgcc cttgatagaa
    15961 gatgtggtcg ccgcgtccag gcgcgtcctt gccgcggtag aagtggttga agcccacctc
    16021 gtacagcagc gcgctcgaag cgtaggtcga gatgtgacca ccgagaccgg agtggtggtg
    16081 attcgcacgg tgcaccatca ccgccgcgtt ccaacgaatg atggcttgga tgcgcttctc
    16141 gagcgctgca tcgcccgggt atttaggctc ttcttcgaca ccgatcgtat tcacgtaatc
    16201 ggtgttgagc ggcaacacgg gctccacccg gtcgcggcgc gcggtctcca acaccttggt
    16261 gagaaggaag cgtgcacgtt cctttcccgt gtgctcgacg acggatttta gcgactcgac
    16321 ccagtcttgg gtctcttggg ggtctcggtc cgaatcaggc ctgtagctgt ccactcgatg
    16381 gtcctcgtgg cgcaatacgt atgccggacc gggcgggtgt tcaagcgtga ggcgcaattc
    16441 ctagatcgcg tgcctgaacc ggggaggttt ccaggccgtt tacggcttgt ggtccgcctg
    16501 gagcggccca agacatcgtt ctcatgccgc gcctcgtttc gtttctgatt ttcttcacaa
    16561 tcttcctgag cgtattgggg ggcgcccact attacgtttg ggcgagattg gtgcgggatc
    16621 cggcgctccc cgcgcagctc agccgtgtgc tcacttgggc aatggtcgcg ttgtttgcca
    16681 gcattcctgc gacatttctg tcgggacggc tgctcggagt ccgggcgggc gggatgctgg
    16741 cctgggtccc gttcgtctgg atggggatgt tatttctgtc actcgtcgtc gtggccgggc
    16801 tcgatttggt acgggcgggg tggctcttgg tgcgcgcggc tggcgacgcg agccccatcg
    16861 atccggagcg tcggctgtgg ttctctcgcg ctttcgctgg ggcagccggg ctgctcgtct
    16921 ctggcctcgg ccttgccgca gtgcgttcgg ggctgtcgag agtgctcgcg cgtgaggtgc
    16981 gggtcgatct ggagcgtctc cctagcgagc tggacggcgt gaccatcgcg caactcacgg
    17041 acgttcacgt gggccccacg attcagcgct ctttcgtgga gcaaatcgtc gagagcacca
    17101 acgccttgaa cccggatgtg atcgcaatca cgggcgactt ggtggacggg tccgtcgaag
    17161 agcttcggga tcacgttgcc cccttggcgc ggctgcgggc gcgttacggc gtctacttcg
    17221 ttacgggcaa tcacgagtac tactcgggcg tggaggaatg gtgtcgtgaa ctcacgcgac
    17281 tcgggattcg cgtgctgcgc aacgagcgcg tgacgatcgg cgtcggtgag gcgagcttcg
    17341 acttggcggg ggtcgacgat taccaggccc gctcgttcgg cgaagatcac ggtcccaacc
    17401 tcggtcgcgc caccgcgggg cgcgaccctt cgcgagagct cgtcctgctc gcccatcagc
    17461 cgcgcgcggt gttcgaagcg cagcaccacg gcgtgggcct ccagctctcg gggcacacgc
    17521 acggtgggca gctcttcccg tggaacttca tggttcggct tcagcagccg gtggtgtcgg
    17581 gcttggcccg cttcggacgc acgctgctct acgtcagcaa cggcaccgga tactggggcc
    17641 cgccgatgcg gctcggcgct cccgccgaga tcacgcggct cgtcctgcgc gcacccgccg
    17701 aagccgtcgc ctgagcccgg ggcacgcgct gaccttgccg cgacgccaag gacacctcgg
    17761 ggcaaagctc aagacacagt tcaggacgca gctcagctca caccggcgcg cgctatcgct
    17821 tgtgcgcccc gctgatgagc aaacccgcga cgtgggctcc tcatcaccca ccgaactgcg
    17881 cattcaactc gagtttttga cggcgagtac ttctctaata ggccagggtg tcgttggtgc
    17941 ggtctgcagc aagcgcgatc tcatttcgcg cctgccaaaa ccctactcac caaccatgtt
    18001 gttacgtctt tcgttttggg ctgccggaac tctcacgctc gctgccgctt gtagcttcga
    18061 agatccgaag agtgacgacg gcggatctgg tggaggcggc gccggcggcc aaaccgcggt
    18121 aggcggacag accaccgcga gcggcggtaa ggccagcggc ggcaccacca gcaaacctac
    18181 gggcggcacc tcgaaccagg gcggcacgac gaccgccgcg ggaacgagcg gcgaagccgg
    18241 tgcgggcgga caagcgggtg agacatcctc agcaagcggt ggaacgagcg gagcggaagg
    18301 cggcgcaggc ggcgaagaag ccggcggagc tcccgaaggc ggcgccggtg gttcggagcc
    18361 gggcggcggc gcgggcgccg ggggtgacac gacggagccg gtagtgattg gctcttcgat
    18421 ctatccgaag ccgcctggag aagacaccgt gccgcaaccg gcgggcacgg tcggtggcct
    18481 cacggtccta aactgggccg gcttcaaatc agccgtttcc tacacgttcg acgactcgaa
    18541 cgagacgcag atcaagcact acgatgatct gaagtcgctg ggcgttcggt acacgttcta
    18601 cctccaaaca ggcaaagggg cgttctcgga cccaacttgg gcgcgcgccg cgcaggacgg
    18661 gcacgagctc ggaaaccaca ccgtacacca cctgaatcag gctgaccttt cccttctccc
    18721 tatgcccacg gacgaagtgg atttagccac caaggcgatt gaaacctcgt acggcgtgaa
    18781 ggtcttcacg atggccggcc cttacggcgc ctcggcttac gaggtgatgg cccaaaaccg
    18841 gttcttcatc aaccgaggcg cttcggacgg catgatgttg gcgagcagtc cagggagtgc
    18901 tttcaacgtc gcctgcttca tcccgccaga cggagcgacc gtagacacct tcaacacgca
    18961 agtgaaccag acccgcacgg cctccgcctg gaaggtgttc ctggtgcacg gcttcaagga
    19021 tgcggtcgtc aaggattggg cctacttacc cgtggattat ccggtgttcc aagacgcggt
    19081 gaactacgcg aagggcttgg gcgacgtgtg gatcgactcg gtcaagaacg tgggtgcgta
    19141 ttggctcggg cagcgcgcct tcaacgccgc caccaagagc gtgtcgggct ccagcacgac
    19201 ctggacctgg cagctcccag cgcacttccc gccgggtaac tacctacgtg tcacggttac
    19261 cggtggaacc ctgaagcagc agaacacgcc tttggcctgg aacccgcgtg gattctacga
    19321 ggtcgcgctc gacgcaaagt cgctgactct atcgccctga gccgatcact ccgatgccgt
    19381 cggctcgaca ccgagctcga cgcagacggc ctgtgctacg cggacgccat cgatcgccgc
    19441 actaacgatg ccgcccgcgt agcctgcgcc ttcacccgtt ggatacagac ccgcgaggcc
    19501 gcgggcttgg agcgtctgcc cgtcacgcac gaggcgcacg gggcaactgg tgcgcgattc
    19561 tacgcccacg agcacgacat cctcggagac gtagccgggc attcgtctgc cgaaggtttg
    19621 cagcgccgcg gacagccgag gagcaatccc aagccccagg ggctcgaata cgtcccaaag
    19681 attcgttggc gtcagacccg ggatgtagct cgtgtcgggc agcgtgctcg aagcctgccg
    19741 acgcacgaag tcggtcgcgc gagtggctgg cgcacgcagc tctccgccgc ccgcgtcgaa
    19801 cgcgatttgc tctagcttgc gctgcaagag cacgcccccg agcgcacccg tgaaaccggc
    19861 tcgctcccag tccgatggct ccacactcac cacgaaaccg ctgttcgcga atggtgagtc
    19921 acgtcgcgcg agactcatgc cgttgacgac gagcccgccc ggctcggtag aagcaggcac
    19981 aatccagccc ccggggcaca tgcagaacga aaatacgcct ctgccctcca cctcgtgcgc
    20041 aactcgatag gcggcgttgg gaagcttggg gtggtccgca ctcgcgccgt actggatgcg
    20101 gttgatcagc ggctgcgggt gctcgatccg aacacccagc gcgaacgcct tgggttcgag
    20161 gctcagcccg agtcgttgcg tgatttctag gatgtcgcgc gcggagtggc cggtcgcaag
    20221 caccacgtga cgcgcgagga tttgctcgcc gccagccagc gcaaccccac agacgcgcgc
    20281 accctggggc ccgtgctcca cgagaatatc gaccacccga gcgccaaacc ggaactggct
    20341 gcccgcagcc tcgattcgct cacgtaacgc ggtgaccacc ttgggcaggc gattgctacc
    20401 gatgtgcggt cgcgcttcgg tgaggatctc cgcgggcgcg ccggccagcg ccaagatctc
    20461 gagcacgtcc cgcacgttgc ctcgcttgtg cgagcgcgtg tacagcttgc catcgctata
    20521 cgtgccggcc ccgccctccc cgaagcaata gttgctgtcc tcgttgacga caccgccgcg
    20581 gtgcaaggcc ttcaagtcgt ggcggcgcgg ctgcacggtt ttcccgcgat cgaccacgat
    20641 cgccgcgata ccgtggcagg cgagttcgta agcgcagaac aaccccgccg ggccatcgcc
    20701 gacgatcacc acctcggggt cgccgcggac gggccgcggt aatcgcagcg cgggaaaccc
    20761 gggttgcccc gatacggtct gaaacccgaa caggagatga aagcgcacgg cccctcggcg
    20821 cgcgtccaaa gatcgtttgc ggagcacgat ttccggtaga tcctcgag
//
LOCUS       DASTKU010000706         4730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_135190, whole genome shotgun sequence.
ACCESSION   DASTKU010000706 DASTKU010000000
VERSION     DASTKU010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135190"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFQ61_24735"
     CDS             complement(<1..198)
                     /locus_tag="VFQ61_24735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957737.1"
                     /translation="MHGNATVAFRPASEAAAFWRPLAVAGLLAAVLGVAAWLQNESVK
                     RPSRPAEPASMSSMNLATPSAA"
     gene            complement(395..1825)
                     /locus_tag="VFQ61_24740"
     CDS             complement(395..1825)
                     /locus_tag="VFQ61_24740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9957738.1"
                     /translation="MRFRHRHLLEFGSRPPRPMALKRQLFAWLVITILFTGLAVGLVF
                     RIARVGDTHGLDPNKLSTFVAQSFAAVQEAPARRAELARALSGALHATVILEDNTGKR
                     EVFGSPCTAPDRSADILVNGKKTGSVSVCLNGLRDARLTGLIAACTVILVLWAAAAKL
                     SRYLTRPLTALIAVTRDLGQGNLKRRVRLRRGNKGELGMLAESINDMAERIEAQLNEQ
                     RELLAAVSHEIRSPLARLRISTELLRGATQDNARLDAIDEEIAGIDALLGKILANSRL
                     DFGTLTCTALSARAVALGALDRHGLPPENLVMAEPDLEVSADPTLLGRALDNLLENAQ
                     AHAQRPLGPEDHHPQSEGTEPKAAPALPSEVVEECAICLHVRPARPGEHPHPERAVAF
                     ELCDGGPGFDPNSLSRIFEPFRKQKQNASGASSARGSLGLGLSLVDRIAKAHGGRAWA
                     KNLPQGGACVGLSIGAEVPLKAATSL"
     gene            complement(1828..2505)
                     /locus_tag="VFQ61_24745"
     CDS             complement(1828..2505)
                     /locus_tag="VFQ61_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9957739.1"
                     /translation="MSLRVLIVDDDTRLFELLQSYFAQNDVFALSAPDGQRGLAMLET
                     ETVDAVLLDVMMPGIDGLTVLRRLRERSRVPVIMLTAKGDETDRVVGLELGADDYLPK
                     PFAPRELLARLRAVLRRTQAELVDSRLAIGNLSIDTQARRVELSGKEVVLTGLEFDIL
                     NALATRQGRVVPRATLLELAGRGDITVSDRTVDVHVSHLRKKLGDDPPRLIRTVRGVG
                     YVFARES"
     gene            complement(2522..2740)
                     /locus_tag="VFQ61_24750"
     CDS             complement(2522..2740)
                     /locus_tag="VFQ61_24750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957740.1"
                     /translation="MRHRFLMVLLGIGAVVGFTSGFVSLAHRHHALGYRSGYGPHAEF
                     EDHVADICTRAAERVYRAKGSASQTAAP"
     gene            complement(2764..3255)
                     /locus_tag="VFQ61_24755"
     CDS             complement(2764..3255)
                     /locus_tag="VFQ61_24755"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HET9957741.1"
                     /translation="MEKQAMFGFLVGAACLGGLFALTMRRPRFGSGHFGHFGHRSPWY
                     AILRRLDTTPGQEKVIRSAVADFMDSAWTMRSKVQSSRSEIADALRAETFDAERIRGV
                     VSQHVEELITLGTSFGVSLGRVHEALDHDQRQRLARLIESHAGGPCLRSTPFFTYSAP
                     HCA"
     gene            3438..>4730
                     /locus_tag="VFQ61_24760"
     CDS             3438..>4730
                     /locus_tag="VFQ61_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9957742.1"
                     /translation="MRIALIYPPPWKIPAKGEKTLSPEQGGPPRDFREGDLDADFFQT
                     PYGLFSLGAQALAAGHQVKVLNLSGFAWSALEELIPKLDAELFGMSCWTANRRGVALV
                     ADLIKKHHPRAEVVVGGPHATPLARELLQHHSAIDRVCVGESDATLMDLVARKERGAS
                     LSEVPGTVYRTGSGIQVAPERPSIANLDTLASPHSYYATHIVMTSRGCPWACTFCGAE
                     TSWGRGFRGHSVPYVLDALEQALARLRVKMVQIKDDTFTTNKKRVIELCRGIRERKLN
                     FLWSCDTRVDVLSEELLREMRLAGCQRLSLGVESGSQRILDQIHKKITKEEILRAVEM
                     AKKYGIHVRFYMMLGNRGETAETFRETLEFLKLAKPHQYIFSCLSIYPGTQDFHDAEA
                     SGWLSREVYFQEAFQELKVPFDANDADTRLMTDWFARNS"
BASE COUNT          871 a         1476 c         1543 g          840 t
ORIGIN      
        1 tgcggcggac ggcgtcgcaa ggttcatcga actcatcgat gctggttcag ctgggcggga
       61 agggcgtttg acgctctcgt tctgcagcca agcagccacg ccgagtaccg ccgccagcaa
      121 ccccgccacc gccagaggtc gccagaacgc ggctgcctca gaggcaggac gaaacgcgac
      181 agttgcattg ccgtgcatgg aaatcctcgc tggaaggagt gttctcgaaa gagccgtggt
      241 gctcacgact cgaggtcgtg agcgcgctcc gctcctcctc ctccgaagca tgcaccgtgc
      301 cgtggaacga ctatccacat ccgatgaaca tcggatgtgg ccacgtggca ccgctgttcc
      361 gctccacagg ccctatctgc ggacccatcg agtgctacag cgaagtcgcg gctttcagcg
      421 gaacctccgc gccaatcgac agccccacac aggctccgcc ttgaggcaga ttcttcgccc
      481 aagcgcggcc gccgtgcgcc tttgcaattc ggtccaccaa gctcaagccc aagccgagcg
      541 agccgcgtgc cgagctggct cccgaagcgt tctgtttctg ttttcgaaac ggctcgaaga
      601 tccgactgag gctgtttgga tcgaagccag gccccccgtc acacagctcg aaagcgacgg
      661 ctcgctccgg gtggggatgc tctcccgggc gagccgggcg cacgtgcaaa caaatcgcgc
      721 attcctccac cacctcactc gggagggcag gtgcggcttt cggctccgtt ccttcgctct
      781 gggggtggtg atcttcaggg ccgagtgggc gttgcgcgtg ggcttgtgcg ttctccaata
      841 agttatcgag cgcgcgcccc aacagcgtag gatccgcgga cacctcgaga tcgggttccg
      901 ccatcactag gttctccggc ggcaggccat gacggtcgag ggcaccgagc gccaccgcgc
      961 gggccgatag cgcggtacac gtgagcgttc caaaatcgag ccgcgaattg gccagaattt
     1021 tgccgagtaa cgcgtcgatg ccggcgatct cttcatcgat ggcgtcgagc cgcgcattat
     1081 cctgagtggc accgcgcagt aactcggtcg aaatgcgtag tcgggcgagg gggctgcgaa
     1141 tctcgtgcga gacggcggcg agcagctcgc gctgttcgtt gagctgggct tcgatccgct
     1201 ccgccatgtc gttgattgat tcagcgagca tgccgagctc gcctttgttg ccccgcctga
     1261 gccgcactct gcgcttgagg ttgccctgcc cgagatcccg cgttactgcg atcagcgcgg
     1321 tcaagggcct tgtcaggtag cgggagagct ttgccgccgc cgcccacaaa accaagatga
     1381 cggtgcaggc tgcgatcaag cccgtcagcc gcgcatcgcg cagaccattc aggcacacgc
     1441 tgacgctgcc ggttttcttc ccgttgacca ggatatcggc gctccggtcg ggggcggtgc
     1501 agggcgagcc aaacacctcg cgtttgccgg tgttgtcctc gaggatcacg gtcgcatgta
     1561 acgctccact caaggcgcgc gccagctccg cgcgcctggc cggagcttcc tgcacggcgg
     1621 cgaagctttg cgccacgaag gtagagagct tattcggatc gaggccgtgc gtatcgccga
     1681 cgcgcgcgat gcgaaagacc agcccaaccg ccaagcccgt gaacaagatc gtgatcacca
     1741 gccaggcgaa cagctggcgc ttcagagcca tggggcgcgg cggacgcgac ccgaactcca
     1801 gcaagtggcg gtgtctgaag cgcatgctca agactcgcgg gcgaacacgt agccgacgcc
     1861 gcgcacggtg cggatcaagc gaggcggatc gtccccgagc ttctttctca gatgcgaaac
     1921 gtgcacatcc acggtgcgat cgctaaccgt gatgtcaccg cgtccagcga gctcgagcag
     1981 cgtggcccgc ggcaccacgc gaccttgacg ggtcgcgagc gcgttcagaa tgtcgaactc
     2041 gagcccggtc agcacgacct ccttgccgga gagctccacg cggcgggcct gagtgtcgat
     2101 gctcaggttg ccaatcgcga gtcggctatc gaccagctcg gcttgcgtac ggcgtagcac
     2161 ggcgcgcagc cgcgcgagca gctcgcgcgg tgcgaagggc ttgggtaaat agtcgtctgc
     2221 gccgagttcc aagcccacca cgcgatcggt ctcatcgcct ttggccgtca gcatgataac
     2281 cggtactcgg ctgcgttctc gaagccttcg cagcacggtc aatccgtcga tcccgggcat
     2341 catgacgtcg agcagcaccg cgtccacggt ctccgtctcg agcatcgcga gcccgcgctg
     2401 cccgtcgggg gcggacagcg cgaagacgtc gttctgagcg aagtagctct gaagcagctc
     2461 gaaaagccgg gtgtcatcgt cgacgatcag gactcggagg ctcattctgg ttcgggggtg
     2521 atcagggggc ggccgtttgc gacgcggatc ctttagcgcg atatactcgc tccgccgctc
     2581 gcgtgcagat atccgcgacg tgatcctcga attcagcgtg gggaccgtag ccggagcgat
     2641 agccgagcgc atggtggcgg tgcgctaacg acacgaatcc agacgtgaag ccaaccaccg
     2701 cgccgatgcc cagtagcacc atcaaaaacc ggtgacgcat gggtcttcct ttctataggg
     2761 cgctcacgcg caatgcggag cagagtaagt gaagaagggg gttgaacgga ggcatggtcc
     2821 gcccgcatgg gactcgatga gacgcgccag ccgctgccgt tgatcgtggt ccagggcttc
     2881 gtggacgcga ccgagtgaga ccccaaagct cgtgccgagg gtgatcagct cctcgacgtg
     2941 ctggctcacc acaccccgga tgcgctcggc gtcgaaggtc tcggcgcgga gggcgtccgc
     3001 gatttcggag cgcgaactct ggaccttcga tcgcatcgtc caggcgctat ccatgaagtc
     3061 cgcgaccgca ctccggatca ctttctcctg cccgggcgtg gtgtccagcc gacgcaggat
     3121 ggcgtaccag gggctgcgat gtccaaagtg cccgaaatga ccggaaccaa agcgagggcg
     3181 gcgcatcgtc aacgcgaaca ggccgcccaa acaggcggcg ccaactagaa aaccgaacat
     3241 tgcttgcttc tccatgctgc gagtgagagc ggtcgagccg ctcggaacga cgccgggcca
     3301 ctccgaatcg gattcgcggg gccggcgaca ggagaaacat gggcgggcag tgttaggggc
     3361 gtagggggca ccgtgtgaag aaacgttaac cctgggcggg ccgagccgcg attcgcggct
     3421 aaactctcgc atcctgaatg cgcatcgctc tgatctatcc gccgccttgg aagattcctg
     3481 cgaagggcga gaagacgctc tctccggagc agggcgggcc tccccgcgac ttccgtgagg
     3541 gagatctcga tgcggacttc tttcagacac cgtatgggct gttctcgctc ggggcccagg
     3601 ccttggcggc gggccatcaa gtcaaggtcc tgaacctgtc ggggtttgcg tggtctgccc
     3661 tggaggagct gatcccgaag ctcgacgcag agttgttcgg catgtcctgc tggacggcga
     3721 accgtcgcgg ggtggcgctc gtggcagatc taatcaagaa gcaccacccg cgggccgagg
     3781 tggtggtcgg cggcccacac gcgaccccct tggcgcgcga gcttctccag caccactccg
     3841 cgatcgatcg ggtgtgcgtg ggggagagcg acgccacgtt gatggacctg gtggcgcgca
     3901 aggagagggg ggcctcgttg agcgaggttc cggggaccgt gtatcgaacg ggttccggaa
     3961 tccaggtggc cccggagcgg ccttcgattg cgaatttgga tacgctggct tcgccgcact
     4021 cctattacgc gacgcatatc gtgatgacgt cgcggggctg cccatgggct tgcacgtttt
     4081 gtggcgcgga gaccagctgg gggcggggct tcagaggcca ctcggtgccg tacgtgctgg
     4141 acgccctcga acaggcgttg gcgcgcttgc gcgtaaagat ggtccaaatc aaggacgaca
     4201 cgttcactac caacaagaag cgcgtcatcg agctgtgccg tgggatccgc gagcgaaagc
     4261 tgaacttcct ctggagctgc gatactcgcg tcgatgtgct cagtgaagag ctgctgcgcg
     4321 agatgcggct cgcgggctgt cagcgcctga gcttgggcgt cgagtctggc tcccagcgca
     4381 ttctggacca gatccacaag aagattacca aagaggaaat cctgcgtgcc gtggagatgg
     4441 ccaagaagta cggcattcac gtgcgcttct acatgatgct cggcaaccgc ggagaaacgg
     4501 cggagacctt ccgcgagacc ctggagttct tgaagctcgc caagccgcac cagtatatct
     4561 tctcgtgcct ctcgatttac ccgggtacgc aggactttca cgatgcggag gcgtccggtt
     4621 ggttgagtcg ggaagtctac ttccaggaag cctttcaaga gctgaaggtg ccgttcgacg
     4681 cgaacgacgc agacactcgc ctgatgacgg actggtttgc tcgcaactcc
//
LOCUS       DASTKU010000707         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1697386, whole genome shotgun sequence.
ACCESSION   DASTKU010000707 DASTKU010000000
VERSION     DASTKU010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697386"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFQ61_24765"
     CDS             <1..223
                     /locus_tag="VFQ61_24765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957743.1"
                     /translation="PSLGKAGLANAGEVWTLRDPNQRTLSVFPAIASKNGGQSLARRS
                     AQAADSSSSFGPHAAPGASPGAANTVTEP"
     gene            complement(263..1624)
                     /locus_tag="VFQ61_24770"
     CDS             complement(263..1624)
                     /locus_tag="VFQ61_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET9957744.1"
                     /translation="MQDAEAIELENLAQSLVERARRAGMQVAEVTARQGYDLSVRVRL
                     GQPELVEEAGHRGVTLRVMRDQRVALSSTSDLTPEGLDRLVSDAVELVDLCEADPFAG
                     PADPEDLCHAPHPDLDLYDATVAEIRGAQALQIATEAERVALASDPRLTLSEGATFSR
                     STGLSAMVLSGGFSGVQRGSYASLSVAPVVEDEGGKKRRGYYWTASRHASGLETAEAV
                     GKEAARRTLRKLGARKVPTGEAAVVFDPDVARSLLGSFAGCILGGSIWRKSSYLVERE
                     GTPVASPLVTIVDDPLIPGAPGSRAYDGEGLRSVRQVVVERGVLKTFLLDSYSGRKLK
                     RKSTASAARSGGVGPSTSNLLLQAGPLSPAQLIKETGTGLYVTEMMGFGFNALTGDFS
                     RGAAGFWIENGELAFPVSEVTISSNLDTMLKGIDALADDLVLRTSTAAPTLRVQRMTI
                     SGT"
     gene            complement(1817..2356)
                     /locus_tag="VFQ61_24775"
     CDS             complement(1817..2356)
                     /locus_tag="VFQ61_24775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957745.1"
                     /translation="MPAPIGPLLGLALGGAFAWVLNDGSTRGKAALTLPPLALVCCFT
                     LLIFAPAAAYFIAFEPDWAYAYFVDSSQRLGPLDTFALLADVTSVPGGFLAVLHAARG
                     PTFSVLLRWIGLPLLVSILFLVILFPRLSVYATYAQFHGDFGTRPVSGSPLGYALIWI
                     TFVVACAAAWTAHSLKRMR"
     gene            complement(2402..>2977)
                     /locus_tag="VFQ61_24780"
     CDS             complement(2402..>2977)
                     /locus_tag="VFQ61_24780"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9957746.1"
                     /translation="NNLAAAMALFATFRSKAELTLQLHELCVASASIAHGIAKKTQHA
                     PPSTAFVCGLLCEIGAMACLVFDGKEYVKLWQHASGDPQNRGELERERYGVSSYEIGR
                     RFLERNSLPPNVSAAVGADLIKDPEQADPLGKITVVARHAPTAIARGVGQPDQLRAGL
                     AELADRVKLPKLDGELLFDICAKVGAFLAPT"
BASE COUNT          582 a          964 c          935 g          496 t
ORIGIN      
        1 tccgagcttg ggaaaagcgg gcttggccaa cgcaggggag gtctggacat tgcgtgaccc
       61 gaaccagcgg acgctctcgg tttttccggc aatcgcgagc aagaatgggg ggcaaagcct
      121 ggctcgacgc tctgcccaag cagcggactc gagctcgtca tttggtccgc acgcggcgcc
      181 aggagcgtca cctggcgccg cgaatacggt cacggagccg tgagcgcgtg gcgcccatca
      241 agcagccccg cgccgcgcaa cttcacgtcc cagagatggt catgcgttgc acccgcagtg
      301 tgggtgccgc ggtgctggtt cgcagcacca gatcatccgc cagagcatct atgcccttca
      361 acatggtgtc caagttcgag gagatcgtga cctcgctgac ggggaaagcg agctcaccgt
      421 tctcgatcca gaagcccgcg gcgccacgcg aaaagtcgcc cgtgagcgcg ttgaagccga
      481 agcccatcat ctcggtcacg tagagcccag tgcccgtttc cttgatgagt tgtgccgggc
      541 tgagtggacc ggcctgcagc aacaagttgc tggtgcttgg tcccacgccg ccgctgcgcg
      601 cagccgatgc cgtggattta cgtttcagtt tgcgaccgct gtagctgtcg agcaggaacg
      661 tcttcagcac ccctcgctcc acgacgacct ggcgtaccga acgcaatcct tcgccatcgt
      721 aagcacgaga gccgggcgca ccagggatca aaggatcatc gacgatcgtc acgagcgggc
      781 tcgccaccgg cgttccttct cgttccacga gatacgagga tttgcgccaa atcgatccgc
      841 cgaggatgca tccggcgaag ctgcccaaga gagagcgggc cacgtccgga tcgaatacga
      901 cggccgcttc acccgtcggg acctttcgcg cacccagctt gcgcagcgtg cggcgagcgg
      961 cctctttgcc gacggcctcc gccgtttcca agccgctcgc gtggcgactc gccgtccagt
     1021 agtagccgcg tcgcttcttt ccgccctcgt cttctacgac cggcgctacg gagagcgagg
     1081 catacgagcc ccgctgaact ccagagaacc ctccggacaa caccatcgcg gacagcccgg
     1141 tgctgcgcga gaaggtggcg ccttcactga gggtcaagcg tggatcggaa gccaaggcca
     1201 cacgctctgc ctccgtcgca atctgaagcg cctgcgcgcc cctgatttcc gccaccgtag
     1261 cgtcatacaa atccaaatct ggatgcggcg cgtggcacaa gtcttccgga tctgccgggc
     1321 ctgcgaacgg atcggcttca cacaagtcaa cgagctccac cgcgtccgac accaagcgat
     1381 ctagcccttc gggcgtcaaa tcgctggtcg aagacaaagc cacccgctgg tcgcgcatta
     1441 cccgtaaggt cacaccgcga tgcccggctt cctcgacgag ttccggctga ccgaggcgta
     1501 cccgcacgct caagtcatat ccctgtcgag ccgtcacttc agcgacctgc atacctgccc
     1561 ggcgggcgcg ttcgaccagg ctctgggcta ggttctcgag ctcaatcgct tcagcatctt
     1621 gcatggcttg cctttgtagc gcgctttgcg gctcggtgtc gcgcgcgcag gccgctttgg
     1681 gctaaaaacc cgccctgggc taaaaacctg cctgggcaaa aatcacggtg cagacgcgag
     1741 tcggcgggct ccagtcgagc gggctgggtc tgcgcccgca gctcggtagt ccgagtgcgt
     1801 gggccgcgag cacctttcag cgcatgcgct tcaacgaatg cgccgtccaa gcggcggcgc
     1861 acgctaccac gaaggtaatc cagatcagag cgtacccaag cgggctaccg gacaccggcc
     1921 gcgtgccgaa atcgccgtgg aattgggcat acgtggcgta cacgctgagg cgaggaaaaa
     1981 gaatgaccaa gaaaaggatg ctcaccagca gcggcaggcc aatccaacgt agaagcaccg
     2041 agaaagtcgg gccgcgtgcc gcatgcaaga ccgccaaaaa tccgcctggc acgctggtca
     2101 cgtcagccag caacgcgaac gtgtccaacg gtcctaggcg ctggctgctg tctacgaagt
     2161 aagcgtaggc ccagtccggc tcgaaagcga tgaagtacgc cgccgccggc gcgaagatca
     2221 gcagcgtgaa gcaacacacg agcgcgagag gtggcagcgt cagcgctgct ttgccacgcg
     2281 tggaaccgtc attcaaaacc caggcgaacg ccccgccgag ggcgagaccg aggagggggc
     2341 cgataggagc aggcacggcg cgcagcagag cacgagcgtg cagacgagag aaggcgttcg
     2401 cttacgtcgg tgcgagaaat gcgcccacct tggcgcaaat gtcgaacagg agctcgccat
     2461 ccagctttgg cagcttcact cgatcggcga gctccgcgag cccagctcgg agctgatcgg
     2521 gctggccgac accgcgggca atggcggtgg gggcgtgtcg cgcaacgacc gtaatctttc
     2581 cgagtgggtc ggcctgctcc ggatccttga ttaggtcggc acccaccgcg gcgctcacgt
     2641 tggggggcag tgaattgcgt tcgaggaaac gccggccgat ctcgtagcta gaaaccccgt
     2701 aacgctcgcg ctcgagctcg cctcggtttt gcggatcccc cgaggcatgc tgccagagct
     2761 tcacgtattc ttttccgtcg aagaccaagc aggccatggc tccgatctca caaaggaggc
     2821 cgcaaacgaa cgccgtgctg ggtggggcgt gctgcgtctt tttggctatc ccatgcgcta
     2881 tcgaggcgga agctacgcac agctcgtgaa gttgcagcgt gagctcggcc ttggagcgga
     2941 aggtcgcgaa cagggccatc gcggcggcca ggttgtt
//
LOCUS       DASTKU010000708        21449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_135482, whole genome shotgun sequence.
ACCESSION   DASTKU010000708 DASTKU010000000
VERSION     DASTKU010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 21449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21449
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135482"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VFQ61_24785"
     CDS             <1..779
                     /locus_tag="VFQ61_24785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957747.1"
                     /translation="QAEQYADPRSLERLDHTAYTLRSGEFSVGLDKGQLGVLDHLQIG
                     TYLLPWFVFPWTSSPIPSGIVKLRMPIAERVALSVQASGIYLSSDALRWAFDIRQETR
                     GSLFVLPVEAAFSLALSPVWTQSGSLTYVASGLQGGTRGNTRLWGAVISSALTFSTYS
                     EFRLSPMFAISLLSRILLRQGGTRVTAEVRQGATTVNLDIGAGPSREILTCVIPGVEL
                     SFGMLHMNLGLGYGSYWIPAVELPVSTAGLVPDVSAYLRF"
     gene            complement(808..1686)
                     /locus_tag="VFQ61_24790"
     CDS             complement(808..1686)
                     /locus_tag="VFQ61_24790"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HET9957748.1"
                     /translation="MHIHLVDGTYELFRNYFAVPSTKAPSGREVGAARGLLRSLLAWL
                     RAGGVTHIAVAFDHVVESFRNDLYAGYKTGEGMDPDLYSQFELAERVTRALGIVTWPM
                     VEFEADDALATFADRAHRDPSVERVWLCTIDKDLAQCVRGERVVCFDRHRNQVLDASG
                     VRSKFGVDPGSIPDYLALVGDSADGYPGLPGFGAKSAAALLTRWHTIDRIPDSAESFD
                     VPLRGAKKLAETLARQRSDAQLFRTLATLRCDVPLPEGIADLEWQGAKRAELEALCEE
                     IGDTTSLERVPRFQPS"
     gene            1863..3839
                     /locus_tag="VFQ61_24795"
     CDS             1863..3839
                     /locus_tag="VFQ61_24795"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9957749.1"
                     /translation="MQSRHLAVWSRVGVATLLAQIAAGCSGEIPSAEEGGAAGGGSVG
                     GTAAGVSPSGARDAGTHLEGGTGGANRPSPAAGAAAGVGGSVSGGSGSVAPVSAPEFA
                     CDKSMTEPVSNQAPVAALRRLTMRELGNSVLDLVRFVGGTDISLQDQIRTSIEKLPLD
                     RREPVPQDPRGSYRRLDQTLQQVHVEALYALSRTVAAALTDESHLASTVGACATDSDS
                     NNDAACLSQFIARFGGRALRRPLAQDEIEFYRLAYGEDPHADRAAYADLIGLFLNSPE
                     FVYFVEHGAEPLANQPGTFSLSAYELASRLSYQLWQTAPDAALLAAAKDGSLLENSGF
                     SAQLDRLLRDPRARATLQEFFEDWVKAEDLPALDAKRDDPIFQAFAGEDLPEPSLRRA
                     LIDDATGLLGYFTWDEPASVGTWFLTQKSFARDARLARIYGAPIWDERAVAARMPPSM
                     PDDARPGLLTRALFLTTGSANTRPIMKGVFIRRQILCDDIPPPPPGANSTLPELRPDM
                     TTREVVEELTEASGSTCAACHATLINALGFATENFDALGRLRVEQSLFDEHGRIVGTK
                     PVDTSGVPRVRLQDSSRLAGAEELMRKVVESGKVEACLARNYFRFTFARWEDLRADGC
                     VLEDMRRGIASGQSFQELMRRALASEAFHSRRFE"
     gene            3911..5473
                     /locus_tag="VFQ61_24800"
     CDS             3911..5473
                     /locus_tag="VFQ61_24800"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9957750.1"
                     /translation="MAKRIIDISRRQFLVGLAGTTLALPVMPSLLTRNAYGQDPTFVR
                     KPRLFWVSTSHGGAFESSMFPGAEVLTGKGQVFADHGFRYGSAVGRVEAGRRAVSAVL
                     SADASRFSEGLAKKMNVLHGLDVPFYIAHNTGLHLGNYARNDGNGADGKNVQKDPRPT
                     IDQILAWSRSFYPELTGVRERAMIMSSEALSWNYSNPEASSGNVENVRGYTSSQQLFD
                     RIFVPPSSSGPVARRPIVDRVLASYKNLRNGNRRLSAADRQRLDDHIARLAELERKLS
                     SASALSCAAAPRPREDAEQARGNDPTSAARYGQLFNDVVVAAFTCQTSRIAALGYGST
                     EAFVAYGGDWHQDVAHQWQDGEKQKLLVSSYQRFFESVFLDLAAKLDVEEAPGMTYLD
                     NSLLVWTQESGMETHGSVSIPVVTFGSAAGYFKTGQYLDYRRTESVASRYDPGAGGEQ
                     YLGLLYNQWLANVLTAMGAPRPEFERWGHRGYGVPYLTVDDWTPPYRQHYGNTSSRYF
                     QIASDPLPNLRA"
     gene            5853..6929
                     /gene="fbaA"
                     /locus_tag="VFQ61_24805"
     CDS             5853..6929
                     /gene="fbaA"
                     /locus_tag="VFQ61_24805"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET9957751.1"
                     /translation="MAASLKLKPGIVTGAQLDELFAYCKERQCALPAVNVIGSHSANA
                     ALAAAREAKAPVIVQFSHSGAQFYAGKFLDNKQHQASIAGAVAGAHHVRTLAQAYGVP
                     VVLHTDHAAKKLLPWIEGLLDAGEAFFKSSGAPLFSSHMLDLSEEPLHENLEISRKFL
                     TRMAKMGMTLEIELGVTGGEEDGVDNSGVENSKLYTQPSEVLAAYDALNPVGSFTVAA
                     SFGNTHGVYSPGNVKLTPVILKNSQDKIAADRKTAGKPVNFVFHGGSGSSQAEIREAV
                     SYGVVKMNIDTDTQWAFTHPVKKYMDEKTAYLQGQLGNPEGPDAPNKKQYDPRAWLHL
                     GEKGMAQRLVEAFKDLNSFGQFEL"
     gene            complement(7016..7951)
                     /locus_tag="VFQ61_24810"
     CDS             complement(7016..7951)
                     /locus_tag="VFQ61_24810"
                     /inference="COORDINATES: protein motif:HMM:NF024595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HET9957752.1"
                     /translation="MWPSAPGGSPGGAPDFRNVVAPVAPPSPPPAAIELSALESFRFA
                     FRQPDLLSNVLFGLLLQFIPLVGPIVRMGWSAETTRRLVFGDARPIPKFSFNDFSKYL
                     ERGVVPFVAQLVVVFVLVIPLVLLGVLAGIMSGALAHQHTELGLVIGIAATVLGIPAF
                     ALYFLFMHSTLLIAELTGDFRLSFSLALHKAYLARCWGKVLVRGLVFGLLTIGVSLVG
                     ILACGIGLYLAIQVVLFASGHLRYQLYNAYRLEGGEAFPTKSPTPTEDEQRELAARYY
                     AGYAQPGYGPGYGSAPQGYGSPPANNSPPQGPAQP"
     gene            complement(7993..9195)
                     /locus_tag="VFQ61_24815"
     CDS             complement(7993..9195)
                     /locus_tag="VFQ61_24815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957753.1"
                     /translation="MLDESERLQRWLTLGTASFYALTVGIGLLLRWDTATAFASSSGA
                     NFAFLRHAHSHAGFYGVLTLCWWEVERRQGTRLLRGWVLLHMACALLAALGFAWMGYR
                     APTIALSTVVAGLWLRVGLQRLSALRGPARPPGSSARLGSAAAMRVRGEWLDATPFGL
                     IAGVALIPAIAVTAKRDFMLSRDLAHVFVCSVLFTVFVPAAFQALGYARRVALYWYVP
                     LALVGAARVVFGSRGPEVVSWLTALLALLFATCVTFVLLTVRRRNRYWYGFWVLPAAI
                     VIGSFVPSENAYSLRIAGIHLLILGPVLTSLLVRTPAPAAYVRNSEPWTARASWLLHW
                     ATLLLMVGALVLPSTLLSMPVSAVTQPVWRHDPIGIAAWACSIFVVTSLGLAFSLASR
                     RLEKPSSE"
     gene            complement(9237..10103)
                     /locus_tag="VFQ61_24820"
     CDS             complement(9237..10103)
                     /locus_tag="VFQ61_24820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957754.1"
                     /translation="MAQHAVACDNQAACQRSSDCAKVGRCSPNEKGICSVVSNDDCKA
                     SELCKLHGKCSAKDGTCAALEDTDCKGAQDCQKLGQCSAYQGSCADLSKSIHAECTKT
                     CESEGLCVMKDGHCQALSRLHCAGTSEEKAEPESPCAKLGLCEAEGGHCVAKEKADCK
                     RSAACKELAKCTPSEGRCIASEEDCKTSAACTSAGRCGSKAGQCVALSNADCRQSGRC
                     KLEGTCSLKEGACVAASSADCQSSAVCTKAKRCQAQDGVCIGAGGAGRGGAGKSGAPD
                     KAGNTTITGLSI"
     gene            10389..11234
                     /locus_tag="VFQ61_24825"
     CDS             10389..11234
                     /locus_tag="VFQ61_24825"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9957755.1"
                     /translation="MNHQLGIAPCVSVLVPAYNEAATIGIVVRHVLSLPIVKEVIVVD
                     DCSTDGTAEVMSEIDDPRLKLLKQPQNAGKTAAIQRALDEVSGDIVIIQDADLEYDPS
                     EIPNVVDPILRGKADVVYGSRFLVRKAARVLYYYHALANHSLTTLSNLLTNLNLTDVE
                     TGYKAFRTEVIRGIPLVSRGFGMEIELTASVAKLGVRIYEVPISYYGRTYAEGKKIGF
                     KDGLSALWYILYFNALEPFTERRKSYFDAARERLARGAERFPRIDTIRPGPRTRRDSV
                     NPRLK"
     gene            11270..12085
                     /locus_tag="VFQ61_24830"
     CDS             11270..12085
                     /locus_tag="VFQ61_24830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9957756.1"
                     /translation="MVQLTPKNVTAGRSMKSAPASNATAYADAGTGTRALELLEDARA
                     YNGWIVDAIRPYLGDVNFELGAGRGTLSALVAESHAIVPFEIAEANREVLRERFRNHP
                     RVAPSEVDLLGCDRWNSVDCIYSANVLEHIPDDLAVLRHAARLLKPGGNFVAFVPAGM
                     WLYSEFDRSIGHVRRYTASDRIRLAHVRAPDAELFLHEWRHLNVPGALGWFLKMRLLG
                     RTELDARDVSRVARLIPLIRRLDRLRLPFGQSVLLVWKRPGPTGRANALSSDR"
     gene            complement(12069..12473)
                     /locus_tag="VFQ61_24835"
     CDS             complement(12069..12473)
                     /locus_tag="VFQ61_24835"
                     /inference="COORDINATES: protein motif:HMM:NF025569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HET9957757.1"
                     /translation="MTDSPTPFPCPCCGHLVFAAPPGSEDICLVCFWEDDADQLRWPN
                     LAGGANAVCLREAQRNYQQFGAIEERFRNDVRPATEEEPLDPDFRPIASGDVFEVQSE
                     EAVWPADLTSLYYWRRPAAPPLVVAPDISDLN"
     gene            complement(12470..13687)
                     /locus_tag="VFQ61_24840"
     CDS             complement(12470..13687)
                     /locus_tag="VFQ61_24840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9957758.1"
                     /translation="MNGQAHRARRTATILALSFVESFSGVLLQRGLYFYTHDVLAMSD
                     RENLALALASGVTYAGGALLSHPLATRHGERRALLAVLTLLFALHTSLAVFPTTWMIA
                     LVFPLLGAVQGAKWPLVETFVSAGHAPAALARVLGRFNATWCLAIPLAMLASGALISS
                     EFRPALFAAAGALNVGSLLLARALPGAPEHLAVAHPERPDPIQLKRFSSLLAAARFGM
                     LLSYALLFLLAPLLPEVFSRLGVAVAWGASLSGLIDLARLVTFSVLGRYRAWRGRTGL
                     LLGMLFLAPAALVLILTTDRLLWLIVGELCFGVAAATLYTAALYYAQLVQNASVEAGG
                     AHESWIGVGFALGPASGLVGSFLQSSSLPSNRATLVALLPLVISFVVLSARHLLAVGW
                     STARETRPTQTSP"
     gene            complement(13684..14970)
                     /locus_tag="VFQ61_24845"
     CDS             complement(13684..14970)
                     /locus_tag="VFQ61_24845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9957759.1"
                     /translation="MTETVPTPSAPLRDMCPLGDALQPSRDVLPSRDVLLSSDFLRIL
                     AVQFTIGFGFSIYFLLPKYLAVELKADAITIGTLSSTPALAAVLASPWIGPLLDRRGR
                     RKPLLAGGLLAGAASLVLLANPSWIALLYAIRIAQGVGFGLCFNAAAALVADLAPTAR
                     LGQAMGLLGGASLITNAIAPAVCESIAHAWGYTPVFALASATIVLGVMIASTLPTERP
                     PPVSLAGALPGARDTGLYFASFVAGAAFGTLITFTQPFALELGATRVSGYLLGYTVGA
                     LFVRVGFGRIADVYGRRRIAALALGVYGVVAFATSQLQPAWLLLFGVAFGLAHGVLYP
                     ALAALAAEGSAASRRGRALTRLNASFNLGTGAMLLIGGRIAQWLGYPGLFTSAGLGIA
                     ACALLFHLSPRRTSSNDWIVGESRPSPDRPASSHPS"
     gene            complement(15530..17221)
                     /locus_tag="VFQ61_24850"
     CDS             complement(15530..17221)
                     /locus_tag="VFQ61_24850"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9957760.1"
                     /translation="MKPLTASESESRDTHPDLLGIVLRGTYRILRVMDQGNMGTVFEA
                     EHERLKRRLAVKVLSPHMAREPEALARFQREAEIISHLNHPNVVQVIDFDSTEAGAPY
                     LVMEFLHGETLAARLEREGRLPLRVAVRLAHQVAAGLAAAHRAGVVHRDLKPANLFLV
                     DLPFERTQVKILDFGISKSNEGRHRRLTGEHAVLGTPDYMAPEQAIGRAAWVDPRADQ
                     YSLGVILYEALTGHTPFDGQDVKELLYQVVAREPTPLTQYAPETPPLVWRVLRRALAK
                     EPDERFPTVMEFASELLQAAGCSPWPSDPPARPLSGFPQSVFPAMPPIESLVALRGEY
                     RHPGSERPQLSEPARDSVVPPERAVSIHPPPIRIPALPNPSSELSKEASQTPAKTSPG
                     KTQPPKVQRADPVDEEIARILDTARSAFARNDLEDACSKIESALQLNQPGVSSRALAN
                     DSAFVECVFIRQLGSLHAHLQISSPQLAGNGPHLSPEQAFLLSRVDGHASVEDLLDLS
                     PLPRRETLRHLVALLRLGLLSRGVPVQRSTPPVQRSTPPVQQSTPPNRSSTPPTR"
     gene            17683..19665
                     /gene="ftsH"
                     /locus_tag="VFQ61_24855"
     CDS             17683..19665
                     /gene="ftsH"
                     /locus_tag="VFQ61_24855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET9957761.1"
                     /translation="MPNALGSRPPPPPEPRPPVVPEQSPNGRTSWRGWVILALLLITF
                     GTWERFAATDETQPLVPYTAFYKSVQAEEVESVVWKGQTVSGKFKKPTTLEGRSLSSF
                     RTLVPNQEDRDLLPLLREKGVIINVRSEEQPLGIQIVLTLLPFALIIGGWVWLSRRAQ
                     TMMGPGGPFAMIKGKSRRFEKEGQVTVKFDDVAGLKSAKQDLQEIVQFLKEPQHFRKL
                     GGKVPRGVLLVGPPGTGKTLLARAVAGEAGVPFFSINGSEFIELFVGVGASRVRELFE
                     EAKKVAPAIIFIDEIDAVGRSRGAGLGGGHDEREQTLNQLLSEMDGFDRNDLTIVLAA
                     TNRPDVLDPALLRPGRFDRRVTIDRPELAARRAILEVHTRGKPLAIDVDLELIAGNTP
                     GFSGADLANLVNEAALNATRRGAEAIARTDFTAAYDKIVLGDLRETKLDSNEKRRVAV
                     HESGHAVVAHFSPNAEPLERVTIIPRGMALGVTQYVPGVDRHLFTEPQLRDNLRMMLG
                     GYAAERLILGSISSGAENDLKRATELAFRMVAHWGMSERVGPVYHEHKTEHPFLGQML
                     ATEGGTSDATVHIIEDETRQILTGALSGAENILRERRSELELLVNALLATETVEKDEL
                     FRLLGPGSAGSASSLPTGLAASSAEALNADPARSRA"
     gene            19684..20103
                     /locus_tag="VFQ61_24860"
     CDS             19684..20103
                     /locus_tag="VFQ61_24860"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HET9957762.1"
                     /translation="MPKSYFLAKSEPFKYPFSRLVEEKRTTWDGVRNFEARNNLRAMK
                     KDDYVLFYHSNEGKEIVGVAKVVKTAYPDPTAEDGDWSVVDMAPVKSLKVPVSLATIK
                     ADAVLRNMQLVRRSRLSVVPVTAQEFARVLELAETKL"
     gene            complement(20203..20391)
                     /locus_tag="VFQ61_24865"
     CDS             complement(20203..20391)
                     /locus_tag="VFQ61_24865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957763.1"
                     /translation="MPIELELLKTERDKLKEGLREVEAELRKMEAELKLMRQREIQTK
                     REIEALGTLIDIKESREG"
     gene            complement(20519..>21449)
                     /locus_tag="VFQ61_24870"
     CDS             complement(20519..>21449)
                     /locus_tag="VFQ61_24870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9957764.1"
                     /translation="SVPEMREPQFQEEARTMEVTLRTADHPLVAPIAERALLIRMDGI
                     HAGSVIALDQFPFGIGRHPTNQLRIDEESISRFHAKISRGAEGFVVDDLGSRNGTFVA
                     GERVSHKLLTDETWLMLGPQVSFRFALRDLREEALMRRLYESSTRDALTSAYNRSHFD
                     ERLRAEVAYAARHGMAASLVLLDIDHFKRVNDTYGHQAGDIVLRELATRSLRSLRAED
                     VFARFGGEEFGVILRGIPLRGAQRLAERLRVSLANTPIQADGHGILATLSAGCASVSC
                     CGSAPTPEEVIRIADRRLYLAKSAGRNRVIAEG"
BASE COUNT         4106 a         6633 c         6943 g         3767 t
ORIGIN      
        1 cgcaggccga gcaatatgcc gatccgcgct cgctggagcg cctggatcac acggcgtata
       61 ccctgcgcag cggtgagttc agtgtgggtt tggacaaggg gcaactcggg gtcttggatc
      121 acctgcagat tggcacttat ctgctgccct ggttcgtgtt cccgtggaca tcctcgccga
      181 ttccgagcgg tatcgtgaag ttgagaatgc caatcgccga gcgggtcgcg ctatccgtgc
      241 aggcgagcgg catttacctg agctcggacg ccttgcgctg ggcattcgat attcggcaag
      301 agacgcgggg ttctttgttc gtgctgccgg tcgaggcggc attttccctg gcgttgagcc
      361 cggtctggac ccagagcggc tcattgactt acgtcgcgag cgggctccaa ggcggtacgc
      421 gaggtaacac gcgcctatgg ggggcggtga tctcgagcgc gctcacgttc tcgacctatt
      481 cggagtttcg gttgagcccc atgttcgcga ttagcctgct cagtcgtatt ctccttcgtc
      541 aaggcgggac gcgtgtgacg gccgaggtgc gccagggcgc taccacggtc aacctagaca
      601 tcggcgccgg cccgtcgcgg gagattctca cgtgcgtgat ccccggcgtg gagctcagct
      661 ttggtatgct tcacatgaac ctgggcctgg gctacgggtc gtattggatt ccggcggtgg
      721 aactgccggt ctcgactgca ggcttggtgc cagacgtctc cgcctacttg cggttctgag
      781 cgcatccgcc gtgtaggacg cgcgagctca gcttggctgg aagcgcggaa cgcgttccaa
      841 actcgtggtg tccccgatct cctcgcacaa agcctcgagc tcggcgcgct tcgccccctg
      901 ccactcgagg tcagcgatcc cctcggggag cgggacatcg cagcgcaagg tcgcgagggt
      961 gcgaaacagc tgcgcgtcac tgcgctgccg ggccaaggtt tccgccagct tcttcgcgcc
     1021 acgcagcggt acatcgaagc tttcggcaga gtcggggatg cgatctatcg tgtgccagcg
     1081 cgtgagcagc gccgcggccg actttgctcc gaagccgggc aatcctggat agccatccgc
     1141 gctgtccccg acgagcgcca ggtagtcggg aatggatccg ggatcgacgc cgaatttgct
     1201 gcgtacgccg gatgcgtcca acacctgatt gcgatggcga tcgaagcaaa cgacgcgctc
     1261 tccacgtaca cattgagcga gatctttgtc gatcgtgcac aaccaaactc gctctacact
     1321 gggatctcgg tgcgcgcgat cagcaaacgt cgcgagcgcg tcgtcggcct cgaattcgac
     1381 catgggccag gtcacgatgc ctagggctcg cgtgacgcgc tcggccagct cgaactgcga
     1441 atacaaatcc ggatccatgc cctctccagt tttgtagccg gcgtagagat cgttgcggaa
     1501 ggactcgacg acatgatcga aggcgacggc gatgtgagtc acgccgcccg cgcgtaacca
     1561 ggccagcagg cttcggagca acccgcgcgc ggcgccaacc tctcgacccg agggggcctt
     1621 ggtgcttgga accgcgaagt agtttcgaaa cagctcgtag gtgccgtcga cgaggtggat
     1681 gtgcatcact gcgtatgccc gcttggggtg agtagggtgg agtggaaggt tggagcgtgc
     1741 gattgccgcg gagaggaatc gctggggacg ggtgtagccc gaacccgagg ggcctgcgac
     1801 ccgccaagaa agcctgagcc ggaggcggtg cttcgaggga cgtacctccg agcatgcaac
     1861 ggatgcagag tagacatctt gcggtctggt cgcgagtcgg tgtggctacc ctgctggcgc
     1921 agattgcggc cgggtgctcc ggagaaatac cttccgcgga agaaggcgga gccgccggag
     1981 gcggttcagt cgggggaacc gctgccgggg tgtccccgag tggcgcacgc gacgcgggaa
     2041 cccatttgga gggagggacc ggcggcgcga acaggccgag ccccgcagcg ggcgcggccg
     2101 caggcgtagg cgggtctgtt tcgggtggct ccggttcggt cgcgcccgtg tcggcacccg
     2161 agttcgcctg cgacaaatcc atgaccgagc cggtgtccaa ccaagcgccc gtcgctgcac
     2221 tccgccggct cacgatgcgc gaactcggca atagcgtgct cgacttggtt cgcttcgtcg
     2281 gcggtaccga tatcagcctg caggatcaga tccgaacgtc catcgagaag ctcccgctcg
     2341 atcggcgcga accggtacct caggatccac ggggcagtta tcggcgtttg gatcagacgc
     2401 tccaacaagt tcacgtggaa gctctgtatg cgctatctcg gacggtagcg gcggccctga
     2461 ccgacgagag ccacctggca tcgacagtgg gagcctgcgc cacggactct gactcgaaca
     2521 acgacgccgc atgcttgagc cagttcatcg cgcgcttcgg gggccgcgcg ctgcggcgac
     2581 cgctggcaca ggacgagatt gaattctatc gcctcgccta tggcgaggat ccgcacgccg
     2641 atcgcgcagc ctacgccgac ttgatcgggc tgttcctcaa ctcgcccgag ttcgtgtact
     2701 tcgtggagca tggggccgag ccgctggcaa accagccggg tacgttcagc ctctccgcat
     2761 acgagctcgc ttccaggctc tcgtaccagc tctggcaaac cgctcctgac gccgccttgc
     2821 tcgcggccgc gaaggacggt tcgttgctcg aaaactctgg cttctccgcg cagctggatc
     2881 ggctgctccg cgatccgcgc gcgcgggcga cgctgcagga gttcttcgaa gactgggtca
     2941 aagccgagga cttacccgcg ctcgacgcca agcgtgacga ccccatcttc caggcatttg
     3001 cgggcgaaga tttgccggag cccagcctgc gtcgagcgct gatcgatgac gctacggggc
     3061 tgctcggtta tttcacgtgg gacgagccgg ccagtgttgg aacttggttt ctcactcaga
     3121 agagcttcgc tcgggacgcc cgcctcgcac gtatttacgg cgccccgatc tgggatgagc
     3181 gggccgtcgc cgcgcgcatg ccgccgagta tgccggatga tgcccgcccg ggactcctga
     3241 ctcgtgcgct cttcctcacc acgggctccg cgaatacgcg gccgatcatg aagggcgtct
     3301 tcattcgtcg ccaaatcttg tgcgacgaca tccctccgcc gccgcccggg gccaactcaa
     3361 ccttgcccga gttgcggccc gacatgacca cgcgtgaggt cgtcgaagag ctcaccgaag
     3421 caagcgggtc gacttgtgcg gcatgccacg cgacgctcat caacgcgctg gggtttgcga
     3481 ccgagaactt cgacgccctc ggacgccttc gcgtcgagca gtccttgttt gacgagcacg
     3541 gacgaatcgt gggcacgaaa ccggtggata ccagcggcgt gccgcgcgtt cgactccagg
     3601 attcgagccg attggcgggc gctgaggagc tcatgcgcaa ggtcgtagaa agcggcaagg
     3661 tggaggcttg tctcgcgcgc aattacttcc gcttcacgtt cgcgcgctgg gaagacctaa
     3721 gggctgatgg atgcgtgctc gaggacatgc gtcgaggcat cgcctccggt caaagttttc
     3781 aggagctcat gcgccgggct ctcgcgagcg aggcgttcca ttcccgccgc ttcgaatgaa
     3841 cggaactcga tctatcgcct cgggccaccc gactgccaac cctgccgctg ccaaccctgc
     3901 cgaacaagtc atggccaaac gaatcatcga catctctcgt agacaatttc tggtcgggct
     3961 cgctggaacg acgctggcgc tgcccgtcat gccctcgctg ctgactcgta acgcgtacgg
     4021 acaggatcct actttcgtgc gcaaaccacg cctcttctgg gtctcgacgt cacacggggg
     4081 agccttcgaa agcagtatgt tcccaggtgc ggaagtgctc acgggaaagg gtcaagtctt
     4141 cgcggatcac ggcttccggt atggctcggc cgtgggtcgc gtggaggcgg gtaggagggc
     4201 cgtctcggcg gtgttgtcgg ccgacgcgag tcggttctcc gaaggtttgg ccaagaagat
     4261 gaatgtgctg cacggcttgg atgtgccgtt ttacattgcg cacaacaccg gtctccatct
     4321 cggaaactac gcgcgaaatg acggcaacgg cgcggacggc aagaacgtgc agaaggatcc
     4381 tcgccccacg atcgaccaaa tcttggcttg gtctcgctcg ttttaccctg agctcaccgg
     4441 cgtccgcgag cgcgcgatga tcatgagctc cgaggcgttg tcttggaact attcgaaccc
     4501 ggaagcgagc tcgggcaacg tcgagaacgt gcgcggctat acgagttcgc agcagctttt
     4561 cgatcgcatc ttcgttcccc cgagcagcag cgggccggtg gcgcggcggc cgatcgtcga
     4621 tcgcgtgctc gcgagttaca agaatctgcg caacgggaac cgccgcttgt ccgccgcgga
     4681 tcggcagcga ctcgacgatc acatcgcgcg tttggcggag ctcgagcgca agctttccag
     4741 cgcgtccgcg ctgagttgcg cggccgcacc ccgaccccgt gaagacgccg agcaagcgcg
     4801 gggcaatgat ccgacctccg ccgcccgcta cggccaactc ttcaacgacg tggtggtggc
     4861 tgcattcacc tgccaaacca gccgcatcgc cgccttgggt tatggttcga cggaggcctt
     4921 cgttgcttat ggaggcgatt ggcaccagga cgtggcgcac caatggcagg acggcgaaaa
     4981 gcaaaagctg ctcgtcagct cttaccagcg cttcttcgag tcggtgttcc tggacttggc
     5041 ggccaagctc gacgtcgagg aggcgccggg catgacttat ctcgacaact cactgctcgt
     5101 ttggacgcag gagagtggga tggagaccca cggctcggtt tcgatcccgg tggttacgtt
     5161 tggcagcgcc gccggttact tcaagactgg gcagtacctg gactatcgga gaaccgagag
     5221 cgttgcatcg cgctatgatc cgggagccgg aggggaacag tatctcggct tgctgtacaa
     5281 tcagtggctc gccaacgtgc tcaccgcgat gggcgcaccg cggccggagt tcgagcgttg
     5341 gggacaccgg ggctatggtg tcccgtacct cacggtggac gactggaccc cgccttaccg
     5401 tcagcactac ggcaacacat cgtcccgtta ctttcagatc gcgagcgacc cgctccccaa
     5461 cttgcgagcc tagggctgac ctgaacgcgc gtgcggagcg gcatttctgg gagcggtttc
     5521 tgcctggttt ctgcccgtcc gggcggcctc gaatggcggt tgtcaccagc gcgtggcgta
     5581 aaccagaggg tttgccgcgt tgtgtctagc tgagagacca gcgctccaga gccgcgcgcc
     5641 tgggctgcgg agcccgctca gcgctttcca agcggcattt tcagcccttg aagcactgct
     5701 tggcgggttt cccgctctgc gccccgacag ctttttatcg ggttcttcca aattgtgctt
     5761 gcgccgagcg tgcggaggcg aaaaacagcg gctgccgagc ccgagccgtg aggcccgcgg
     5821 acccggccgt catacgaaag aaagaaggaa cgatggccgc atctctcaaa ctcaaacccg
     5881 gaatcgtgac cggagctcag ctcgacgagc tctttgctta ctgcaaggag cgccagtgtg
     5941 cattgcccgc ggtgaacgtg attggctcgc actcggcgaa cgctgcgcta gcggcggcgc
     6001 gcgaggccaa ggccccggtg atcgtgcagt tctcgcactc gggcgcgcag ttttacgccg
     6061 gcaagttcct ggacaacaag cagcaccagg cctcgatcgc cggcgccgtc gcgggcgcgc
     6121 atcacgttcg tacactcgcc caggcctacg gggtgccggt ggtgttgcac acggatcacg
     6181 ccgccaagaa gctgctgcct tggatcgaag ggctgctcga cgcgggcgag gcgttcttca
     6241 agagctcggg cgcgccgctg ttcagctccc acatgctcga tctctccgag gagcctctgc
     6301 acgagaatct cgagatcagc cgtaaattcc tgacccgcat ggcgaagatg gggatgacgc
     6361 tggagatcga gctcggcgtt accggtggtg aggaagacgg cgtagacaac tcgggcgtgg
     6421 agaactccaa gctctacacg caaccttccg aggtgttggc cgcttacgac gcgctcaatc
     6481 ccgtgggctc gttcacggtc gccgcttcgt tcggcaacac gcacggcgtg tactctccgg
     6541 gcaacgtcaa gctgacgcct gtcatcttga agaactcgca ggacaagatc gctgcggatc
     6601 gcaagactgc gggcaagccc gtcaacttcg tgttccacgg cggctccggc tcgagccagg
     6661 ccgaaatccg cgaggccgtg tcttatggcg tcgtgaagat gaacatcgac acggatacgc
     6721 agtgggcgtt cacgcatccg gtcaagaagt acatggacga gaagacggcc tatctgcagg
     6781 gccagctggg caatccggaa ggtccggacg ctccgaacaa gaagcagtac gacccgcgcg
     6841 cgtggctaca cctcggcgag aagggcatgg cccaacgctt ggtcgaggca ttcaaggacc
     6901 tgaactcctt cggtcagttc gagctctgag ttagagccgc tcggtggagc gcccatctcg
     6961 ggattggcgc tccacgcgag accaccgcat tccgcgaacc ggttcagtac cgagctcagg
     7021 gctgagctgg gccctgcggg ggagagttgt tggcgggggg tgagccgtaa ccttgcggcg
     7081 cggagccgta accgggcccg tatcccggct gcgcgtagcc ggcgtagtat cgcgcggcca
     7141 gctcgcgttg ctcgtcttcg gtcggggtag gcgacttggt tggaaacgcc tcaccgccct
     7201 cgaggcgata cgcattgtag agttgatagc ggagatggcc gctcgcgaac agcaccactt
     7261 ggatcgcgag atacaagcca atgccacacg ccaatatacc caccagactc acgccgattg
     7321 tgagtaggcc gaacacgagt ccgcgcacga gcaccttgcc ccaacatcga gccagatagg
     7381 ctttgtgaag ggccagggag aaactcagtc gaaagtcgcc ggtcagctct gcaatcagca
     7441 gcgtgctgtg catgaagagg aagtacagcg cgaacgccgg gatgccgagc accgtcgcgg
     7501 cgatgccgat gacgagcccg agctccgtgt gttggtgggc tagcgcgccc gacatgattc
     7561 cggcgagcac tccgagcagc accaaaggaa tcacgagcac gaacacgacc acgagctggg
     7621 ccacgaaggg caccacgccg cgctccaagt acttgctgaa atcgttgaac gagaacttcg
     7681 gaatcgggcg cgcgtctccg aacaccaagc ggcgcgtggt ctctgccgac cagcccatgc
     7741 gtacgatggg gccaaccagt gggatgaact ggagcaataa gccgaacagc acgttgctca
     7801 gcaaatcggg ctgccgaaac gcaaaccgaa agctctcgag ggcgctgagc tcgatagccg
     7861 caggcggcgg cgacggcgga gcgacgggcg cgacgacgtt tcgaaagtct ggggctccac
     7921 ctggcgagcc tccgggagca gacggccaca tgtggctaga ctaacgcaaa gtcgcgggga
     7981 gctcagcgcc attcattcgg acgagggttt ctcgagccgg cgtgaggcga gcgagaaggc
     8041 gagaccgagc gaggtgacca cgaagatgga gcaggcccaa gctgcgattc cgatcggatc
     8101 gtggcgccaa acgggctgcg tgacggcgct gaccggcatc gacagtaggg tggacggcaa
     8161 gaccagggct cccaccatca ggaggagagt ggcccagtga agcagccagc ttgcgcgcgc
     8221 cgtccagggc tcggagttgc gcacgtatgc agcgggcgcc ggtgtacgca ccagcagcga
     8281 ggtgagcacc gggccaagaa tcagcaggtg gatgccggcg atgcgcaacg aataggcgtt
     8341 ttcgctcgga acgaagctgc cgatcacgat tgcggcgggc aacacccaaa agccgtacca
     8401 gtatcggttg cgtcgcctta ccgtcagcag cacgaacgtg acacaagtcg cgaacaacag
     8461 cgccagcagt gccgtcaacc aactgacgac ctcagggccg cgcgagccga agaccacgcg
     8521 agcggcgccc accaaggcca gcggaacgta ccaatacagc gcaacacggc gtgcgtagcc
     8581 gagcgcttgg aaggcggcgg gcacgaacac ggtgaagagc acgctgcaaa cgaacacgtg
     8641 cgccaagtcc cgtgacagca tgaaatcgcg cttcgccgtg accgcgatgg ccgggatcag
     8701 cgccacgccg gcaatcaaac cgaacggagt ggcatccagc cattcgccgc gaacacgcat
     8761 cgcggcggcg ctaccgagac gggccgagct gccgggtggg cgggctgggc cgcgcaaggc
     8821 ggagagacgt tgtaggccca cgcgcagcca aagcccagct acaacggtgg aaagagcgat
     8881 ggtcggagcg cggtagccca tccacgcaaa accgagcgcc gcgagcagcg cgcaagccat
     8941 gtgaagcaag acccaaccgc gcagcaaccg cgtcccctgg cgccgctcga cctcccacca
     9001 gcagagcgtt aggacgccgt agaagccggc atgtgagtgg gcgtggcgca agaacgcgaa
     9061 gttcgccccc gaactactcg cgaacgcggt agcggtatcc cagcgcaaaa gcaggccgat
     9121 gcccaccgtc agggcgtaga atgaggcggt gcccagcgta agccaccgct gtagacgctc
     9181 agactcatcc aacacggatt cgccttgcca tgagaccggc ggtcgatctc gccttgctag
     9241 atcgagagcc cagtgatggt ggtgttcccg gccttgtcgg gggcgccgga tttgcccgcg
     9301 cctcccctgc cggcgccacc ggcgccaata cacacaccgt cttgggcctg gcagcgcttg
     9361 gccttggtgc aaacggccga gctctgacaa tccgccgagc tcgcggccac gcaggcccct
     9421 tccttcaagc tgcaggtccc ttcgagcttg cagcgaccgg actgtcggca gtctgcgttg
     9481 ctcagcgcga cgcattgccc cgctttagag ccgcagcgac cggcgctggt gcacgcggcg
     9541 gaggtcttgc aatcctcttc gctcgcaatg caacgaccct cgctgggggt gcacttagcc
     9601 agctctttac aagccgcaga gcgtttgcag tccgctttct cttttgccac gcagtgcccc
     9661 ccctcggctt cacacaggcc gagcttggcg caagggctct ccggttccgc tttctcttcg
     9721 ctggtacccg cgcaatgcag gcgagacagc gcttggcagt gcccatcctt catcacgcag
     9781 agcccttcgc tctcgcaagt tttggtgcac tcggcatgaa tggacttgct cagatcggca
     9841 cagctgcctt gataggcgct gcattgtccg agcttttggc aatcttgagc acccttgcaa
     9901 tcggtgtcct ctagagccgc acaggtaccg tctttggcgc tgcatttgcc gtgcagcttg
     9961 cagagctcgg acgccttgca atcgtcgttg ctcacgacgc tacagatccc cttctcattg
    10021 ggcgagcagc gccccacctt ggcacagtcc gaggagcgct ggcaggccgc ttggttgtca
    10081 cacgcgaccg cgtgttgggc catggccagg agcaaggcgc tgcgccaaat ccaacccagg
    10141 cctcttcgct ggtgaaaaag agctgaaagg gactgcggaa tcgaggcgct cgggcttgcc
    10201 atgtgtgtcc tctccatgtg tcatctcgaa gggcggcgga gcgtactcga ttttgcaact
    10261 ttggctgcga tcgttcggta acctcgaaac ttccatgtgg ggcggctttt tttgccagaa
    10321 aggatcggct accttccaaa ctggtcccga agaagcaccc cgatccgcca ccaaggctcc
    10381 gaaccgctat gaatcaccaa ttgggtatcg ccccttgtgt cagcgtcctg gtgcccgcgt
    10441 ataacgaggc cgcgacgatt gggatcgtgg tccggcacgt tctcagtttg ccgatcgtga
    10501 aagaggtcat cgtcgtggac gactgctcga cggacggcac tgccgaggtc atgagcgaaa
    10561 tcgatgatcc gcgactcaaa ctgctgaagc agcctcagaa cgccggaaaa accgcggcca
    10621 ttcagcgcgc gctcgacgag gtttcggggg acatcgtgat cattcaagac gcggatctgg
    10681 agtacgatcc atcggagatc cccaacgtcg tagaccctat tctgcgaggt aaggcggacg
    10741 tggtgtatgg cagccgtttt ctggtgcgaa aggcggcgcg ggttctctac tactaccatg
    10801 cgctggccaa tcactccctc acgacgctat cgaacctgct caccaatctg aatttgaccg
    10861 acgtcgagac ggggtacaaa gcttttcgta ccgaagtgat tcgtggcatt ccgctggtca
    10921 gccgcggctt cggcatggag atcgagctta cggccagtgt tgcgaagctg ggtgttcgca
    10981 tctacgaagt gcccatctcc tactatgggc gcacgtacgc ggaggggaag aagattggat
    11041 tcaaggacgg cttgtccgcg ctctggtaca ttctctactt caacgctctg gagccgttca
    11101 ccgaacgtcg caagagctac ttcgatgccg cgcgcgagcg gctggcgcgc ggtgcggagc
    11161 gcttcccccg catcgacacg attcgtcccg ggccgcggac gcgcagggat tcagtcaatc
    11221 cacgcctcaa gtgagggccg gcggtggcgc ccgccactgc gcgagatgca tggtccagct
    11281 caccccgaag aacgtcaccg caggcaggtc catgaaatcc gcgccggcca gtaacgccac
    11341 ggcctatgcc gatgccggca cgggcacgag ggcgctcgaa ctgctcgaag acgcgcgcgc
    11401 gtacaacggt tggatcgtgg acgcgatacg gccctacttg ggcgacgtca atttcgagct
    11461 gggggcgggg cgcggcacac tgagtgcctt ggtcgcggag tctcacgcga tcgtgccatt
    11521 cgagattgcg gaggccaacc gggaggtctt gcgagagcgc tttcgaaacc atccgcgcgt
    11581 ggccccgagc gaggtcgatc tgctcggctg tgataggtgg aacagcgtcg actgcattta
    11641 ctcggccaac gtcctcgagc acattcccga tgatctggcc gtgttacgcc acgcggcgcg
    11701 cttgctcaaa cccggtggaa acttcgtggc cttcgttccg gcaggcatgt ggttgtactc
    11761 cgagttcgac cgctccatcg ggcacgtgcg tcgctatacg gcaagcgacc ggatccgatt
    11821 ggcgcacgtg cgcgcccccg acgcggagtt gttcctgcac gagtggcgcc acctcaacgt
    11881 cccgggtgcg ctgggctggt tcttgaaaat gcgcctgctc ggacgaactg aactcgatgc
    11941 gcgcgacgtg agccgcgtgg ctcgtctgat ccccttgatt cgcaggctgg accgattgcg
    12001 tttgccgttt gggcagagcg tgctcctggt ttggaaacgg ccggggccaa ccggtcgagc
    12061 caacgcgctc agttcagatc gctgatatcc ggagccacga cgagcggcgg agcggcgggg
    12121 cgacgccagt agtacaagct cgtgagatcg gcgggccaca cggcttcctc actctggacc
    12181 tcgaaaacat ccccgctggc aattgggcga aaatccggat cgagtggctc ctcctcggta
    12241 gcgggtcgca cgtcgttgcg gaagcgctct tcgatcgcgc cgaattgctg gtaattgcgc
    12301 tgcgcctctc gcaaacagac agcattggcg cccccggcaa ggttcggcca acgcagctga
    12361 tccgcatcgt cttcccagaa gcagaccagg caaatgtcct ccgagcctgg cggcgcggcg
    12421 aacacgaggt gaccgcagca ggggcatggg aaaggagtag gtgaatcggt catggcgagg
    12481 tttgggtggg gcgcgtttcg cgagctgtgg accaacctac ggccagcagg tgtcgcgccg
    12541 aaagcacgac gaacgatatc accaacggca acagcgcgac cagggtcgcg cgattggagg
    12601 gcaagctcga agactgcagg aacgaaccga cgaggccaga ggcggggccc aaggcaaaac
    12661 cgactccgat ccaactctcg tgggcaccgc ccgcctccac cgaggcgttc tggacgagct
    12721 gtgcgtagta caatgccgcc gtgtaaagcg ttgcggccgc cacgccgaaa cacagctcac
    12781 cgacgatgag ccaaagcagt cgatcggtcg tcaaaatcaa cacgagcgcg gccggcgcca
    12841 ggaacagcat gccgagcaac agcccggttc gcccacgcca tgcgcggtag cgtccgagca
    12901 cggagaacgt aacgagacgc gccaagtcta tcagtccgga gagggacgct ccccacgcca
    12961 cggccacgcc caagcgggag aatacttcag gcagtaacgg ggccagcagg aagagcagcg
    13021 cgtaggacaa caacatgccg aagcgagcgg cggccaacag cgaagagaag cgcttgagct
    13081 ggatgggatc ggggcgctcg gggtgggcga ccgcgaggtg ttcgggcgct cctggtagag
    13141 cacgggccaa cagcaacgat cccacattca acgcacccgc ggccgcgaac agagcggggc
    13201 gaaactcgct gctaatcagc gctcccgagg ccagcatggc cagcgggatg gcgagacacc
    13261 aagtggcgtt gaagcggccc aagacccgcg ccaaagcggc gggcgcgtgg ccagcgctga
    13321 cgaaggtctc gaccagcggc catttcgcgc cctgcactgc gccgagcaac gggaacacga
    13381 gcgcaatcat ccatgtcgtc gggaacactg ccaaggacgt gtgcagagca aacaagagcg
    13441 tgagcacggc gagcagcgcc cggcgctcgc catgccgggt cgccagaggg tggctgagca
    13501 gcgcgccgcc tgcataagta acaccggagg cgagcgcgag cgccaagttc tcgcgatcac
    13561 tcatggccaa aacgtcgtgc gtatagaagt acaggccgcg ctgcagcaat acaccgctga
    13621 agctctcgac gaagctgagc gccagaattg tcgcggttct gcgagcccgg tgagcttgac
    13681 cgttcatgag ggatgcgagg atgcggggcg atcgggggag ggccgggact ctcctacaat
    13741 ccagtcgttg gaggaagtgc gccgtggcga gaggtgaaac agcagtgcac aggcggcgat
    13801 acctagccct gcgctcgtga atagcccggg gtacccgagc cactgtgcga tccgcccgcc
    13861 gatcagcagc atggcgcctg taccgagatt gaacgaggcg ttgagccgtg tcagcgctcg
    13921 accgcgtcgc gaagcggcgc tgccctcggc ggccagagcg gccagggctg gatagagcac
    13981 gccgtgagcc agcccgaagg ccactccgaa gagcagcaac caggcgggtt ggagctgact
    14041 ggtcgcgaac gccaccaccc cgtacacgcc gagcgccaac gccgcgatgc gacggcggcc
    14101 gtatacatct gcgatgcgcc cgaagccgac ccggacgaac aacgcaccca ccgtatagcc
    14161 caacaagtag cctgacacgc gcgtggcgcc gagctcgaga gcgaacggtt gagtgaacgt
    14221 gattagggtg ccaaacgcgg cgcccgccac gaagctcgcg aagtagagtc cggtatcgcg
    14281 cgcgccgggt agcgctcccg cgaggctaac cggtggtggc ctctcggtag gcaaggtgct
    14341 ggcgatcatt actccgagca cgatagtcgc gctggccagc gcaaacacgg gggtatatcc
    14401 ccaagcgtgc gcgatcgact cacaaactgc gggcgcgatc gcgttggtga tcaaagatgc
    14461 accacccagc aaacccatgg cctgcccgag ccgtgccgtc ggtgcaagat cggcgaccaa
    14521 cgccgcagcg gcattgaagc agagtccaaa ccctacgcct tgcgcgatgc ggattgcgta
    14581 gagcagcgcg atccacgatg gattagcaag caataccagg gaggctgcgc cggcgagcag
    14641 cccacccgcc agcaacggtt tacgccggcc gcgccgatcg agtagcggtc caatccacgg
    14701 gctggccagc acggccgcca acgctggtgt ggagctcaga gtgcctatcg taatggcgtc
    14761 cgccttgagc tcgaccgcca agtactttgg caacaagaag tagatcgaaa agccgaagcc
    14821 aatcgtgaat tgcaccgcca agatgcgcaa aaaatcgctg ctgagcagta cgtctctgct
    14881 cggcagtacg tctctgctcg gctgcaatgc atctccgagc gggcacatat cgcgaagcgg
    14941 tgcagacggc gtgggcaccg tttcggtcac tgcgtgcgac ctcgccgtag ggcgagatat
    15001 cgatgcttcg ccgagacctg gagtgcagga tagcaaggcg gccgagctgg gtaatggaag
    15061 cgagtctcgc gtcgatggtg gcgacatgac tgaagagctc ccgcccgatc gatgagagct
    15121 ccaaaagcga aaaaccctct cgatcgctgg cgatggagag ggtgttcgga gcggggacta
    15181 ttaagctctc agctcgaatt caccctatcc aggcctttaa cgacctgaat ggtcagatgc
    15241 aggcgcttct gcggtacacc cgagagtgcg acgcaaaggc tggcctgggt cgaccagtga
    15301 atacgggcgg agtgaaggaa catctaaagt cgggcgtaac ttagtgtgtt tcgctcgaac
    15361 agtcaaatgc cgggacgcat gcgaacccca aattgatgac ccacgctcac ggcgtcgcgt
    15421 gatttcgatc cggagcctca gtgagtgaat agtacgagca ccgctaaagg tgggttcatc
    15481 ccgcgttgag ccgcagtacg gcgaacaagt cagagcgcct aggcggtagt cagcgcgtag
    15541 gcggggtgct gctccgattc ggtggggtgc tttgctgaac tggaggcgtg cttcgctgaa
    15601 ctggaggcgt gcttcgctga actggcacgc ccctcgagag caatccgagc cgcaacagtg
    15661 ctaccagatg gcggagtgtt tcacggcgag gcagaggaga caggtccagt aagtcttcga
    15721 cgctcgcgtg tccgtccacg cgcgataaca agaatgcttg ctccggggag aggtggggac
    15781 cgttgcccgc gagctgtggg gaggaaatct ggagatgggc atgcagcgac ccgagctgac
    15841 ggatgaagac gcactctacg aacgcggagt cattggcgag cgcacgcgag ctgactcctg
    15901 gctgattcag ttggagtgcg ctctcgatct tgctgcaggc gtcctcgaga tcgttgcgtg
    15961 cgaaagcgct gcgggccgtg tccaggatcc gcgcgatctc ctcatccacg ggatcggcac
    16021 gctgcacttt ggggggctgc gtcttacccg gcgaagtttt cgccggagtc tgcgatgctt
    16081 ccttactcag ttccgacgaa ggattgggca gggccgggat tcgaatcggt gggggatgaa
    16141 tggatacggc ccgctcgggc ggcacgacgc tgtcgcgagc gggctcgctg agctgcgggc
    16201 gttcgctgcc cgggtgccga tactcgccgc ggagcgcgac caagctctcg atgggtggca
    16261 tcgctgggaa taccgactgg gggaagccgg acaagggtcg agccggggga tcgctaggcc
    16321 agggcgaaca gcccgcggcc tgcagcagct cactcgcgaa ctccatgacg gtcggaaatc
    16381 gttcatcggg ctccttggcg agcgcgcggc gcagcacccg ccacacgagc gggggcgtct
    16441 cgggcgcata ctgcgtgagc ggcgtcggtt cccgggcgac gacctgatag agcagctcct
    16501 tgacatcttg gccgtcgaac ggagtgtgcc ccgtgagcgc ctcgtataga atcactccta
    16561 gggaatactg atccgcacga ggatccaccc aagcggcgcg gccaatcgct tgctccggtg
    16621 ccatgtagtc tggagttccg agcacggcgt gctcaccggt gagcctacgg tgccgaccct
    16681 cattgctctt gctgatccca aaatccagga tctttacctg cgtgcgctcg aacgggagat
    16741 cgacgagaaa caagttcgca ggcttgagat cgcgatgcac gacccccgcg cgatgcgccg
    16801 ctgcgagccc agccgcgact tgatgagcca gtctgaccgc gacgcgcagc ggcaaacgtc
    16861 cctcgcgctc caagcgcgcg gcaagcgtct cgccatgcag gaactccatc accagatacg
    16921 gggcgcccgc ctccgtgctg tcgaaatcga tcacttgcac cacgttgggg tggttcaaat
    16981 gactgatgat ctcggcttcg cgctggaagc gagccagcgc ctccggttcg cgcgccatat
    17041 ggggcgaaag taccttgacg gcgaggcgac gtttcagccg ttcgtgctcg gcttcgaaca
    17101 ccgtgcccat gttgccctgg tccatcacgc gcaagatccg atacgtcccg cgtaggacga
    17161 ttccgagcag atctggatga gtgtccctgg attcggattc cgatgcggtg agcggcttca
    17221 tgtcacggct tgtgttcgtc cgcgggtgcg cgcgaagcgt tcccgattgc caattttcag
    17281 ggtagctcgc cgcttcggct ggcgtcgagc gaatcagcgg tgagtaacac taagagctcg
    17341 cacctttggg atactcactt tcacccgtga cgcgtaattg tcgacggtcg cggcggttct
    17401 cggcagtcga acttcggcgc tgagagttcg tacatggcag ttggcgttag acgtgatgtt
    17461 cctcgtcacg gtttcttcgc ccgtcgcttt ctggagggcg ccttctttgc gctcatcgtg
    17521 caaccgcgta gcaaggccgt tacgtgcgcc acatgacccg tctcagtgcg tgcgagcgga
    17581 gagacatcga ggcggatggc agaaatcgta cgattggttc gcgggccggt gagccgtcac
    17641 tcgacgagcg caacaagcgt cattactttc tccgacacac ccatgccgaa cgctctcgga
    17701 tctcgcccgc cgcctcctcc cgaaccccgc ccgccggtgg tgcccgagca atcaccgaac
    17761 ggacgaacga gttggcgtgg ctgggtcatc ttggcactgc tgctaattac gttcggaacc
    17821 tgggagcgct tcgcggccac ggatgaaacc caaccgctcg tgccttacac cgcgttctac
    17881 aagtcggttc aggccgaaga ggtggaatcg gtagtttgga aggggcagac ggtcagcggc
    17941 aaattcaaga agcccaccac gctggaaggg cggtcgctca gttcgtttcg aacgctggtt
    18001 ccgaatcaag aggaccgaga tcttctgccc ctcttgagag agaagggtgt gatcatcaac
    18061 gtcaggagtg aagagcagcc cttagggatt cagatcgtcc tgactttgct gccgttcgcg
    18121 ttgatcatcg ggggctgggt ttggctctcg cggcgcgccc aaaccatgat ggggcccggt
    18181 gggcccttcg cgatgatcaa aggaaagagc cgtcgcttcg aaaaagaggg tcaggtcacc
    18241 gtcaaattcg atgacgtagc ggggctaaag tccgccaagc aggatttgca ggagatcgtt
    18301 caattcctga aggagccaca gcacttccgg aagctgggag gcaaggtgcc tcgcggcgtg
    18361 ctgctcgtgg gccctcccgg tacgggaaag acgttgctgg cccgtgcggt ggctggtgaa
    18421 gccggcgttc cgttcttctc gatcaacggg tcggagttca tcgagctgtt cgtgggcgta
    18481 ggtgcgtcac gcgttcgaga gctattcgag gaggcgaaga aggtagcccc cgcgattatc
    18541 ttcatcgatg agatcgacgc cgtcggccgc tcccgtggcg cgggtttggg tggaggtcac
    18601 gacgagcgcg aacaaaccct gaatcagttg ctctccgaga tggatggttt cgaccgcaac
    18661 gatctcacga tcgtgctcgc ggccacgaac cgcccggacg tgctggatcc ggctctgctc
    18721 cgcccaggcc gcttcgatcg ccgcgtgacc atcgatcggc ccgagctcgc ggctcgcaga
    18781 gccatcctcg aggttcacac gcgaggcaag cccctggcca tcgacgtcga cctcgagctg
    18841 atcgcgggta acactcccgg attctcgggc gcagacttgg cgaacctggt caacgaggcg
    18901 gccctgaacg cgactcgtcg cggcgccgag gcgattgccc gcacggactt tacggccgcc
    18961 tacgataaga tcgtgctcgg cgatctacgc gaaaccaagc tcgattccaa cgagaagcgc
    19021 cgggtggcgg tgcacgagtc cggacacgcc gtagtggctc atttctcgcc caacgcagag
    19081 cccctcgagc gcgtcaccat cattccgcgg ggcatggcgc tgggcgtgac ccagtacgtg
    19141 cccggggtag atcggcacct gttcacggaa cctcagctgc gtgacaacct gcgcatgatg
    19201 ttgggcggat atgcggcgga acgactgatc ctgggcagca tctcttccgg ggcggagaac
    19261 gacttgaagc gcgcgaccga gctcgcgttc cgcatggtgg cccactgggg catgagcgag
    19321 cgagtggggc ccgtctacca cgagcacaag accgaacacc ccttcctggg gcagatgctg
    19381 gccacggagg gcggtacgag tgatgcgacc gtccacatta tcgaagacga gacgcgccaa
    19441 attctgaccg gcgcgctctc gggcgccgag aacattctgc gcgaaaggcg cagcgagctc
    19501 gagttgctcg tcaacgcgct gcttgccacc gagaccgtcg agaaagacga gctgttccgg
    19561 ttgttaggac ccggttccgc cggctctgct tcgagcttgc cgacggggct ggcggcctct
    19621 tccgcagagg ctctgaacgc ggatcccgct cgctcacggg cgtgatacag cggagctcgg
    19681 acgatgccca agagttactt cctcgccaag agcgaaccgt tcaagtaccc gttttcgcgg
    19741 ttggtcgaag agaagcggac gacttgggat ggagtgcgga acttcgaggc gcgcaacaac
    19801 ttgcgcgcca tgaagaagga cgactacgtg cttttttacc actccaacga gggcaaggag
    19861 atcgtcgggg tggccaaggt cgtcaagacg gcttacccgg accctacggc cgaggacggg
    19921 gactggtctg tcgtggatat ggcgccggtc aagtccctca aggttcccgt ttccttggcg
    19981 accatcaaag ccgacgcggt gctgcgcaac atgcagctcg tgcggcgttc acggttgagc
    20041 gtggtacccg ttaccgcgca agagttcgct cgcgtactcg aactcgccga aaccaagctc
    20101 taacgagccg agctcagcgg ttcgtcgatc ttttcagacc ggctctgcca aagttcaacg
    20161 ccaaagttca acgggccctg ccgaagccta gtcgaggagg cttcagcctt cgcgagactc
    20221 tttgatgtcg atcagcgtgc cgagcgcttc gatctcgcgc ttggtttgaa tctcgcgctg
    20281 gcgcatcagc ttcaactcgg cttccatttt gcgaagctcg gcttcgactt cacgcaaacc
    20341 ttccttcagc ttgtcgcgtt cggtcttcaa cagctcgagc tcgatgggca cgatgatcct
    20401 ccgtaaaagc aggtctgctc gagactcgta gcaaactccg gccgagccga aaatcgcttt
    20461 cgccgcccga cgagaaaaat cgccagcttc ggcccggcgc tcggcgttgg atccgcggct
    20521 agccctcggc gattacgcgg ttccgacccg cgctcttcgc gaggtagagg cggcgatcgg
    20581 caatgcggat gacctcttcg ggcgtcggtg cgctgccaca gcacgagacc gaggcgcagc
    20641 ccgcgctgag tgtggcgaga attccatggc catcggcctg gataggagtg ttagcgagcg
    20701 agacgcgcaa tcgttctgcg agccgctgcg caccgcgtag gggaatcccg cgcaagatca
    20761 cgccgaactc ctcgccgccg aaacgtgcga aaacgtcctc cgcccgcagc gaacgcagag
    20821 aacgcgttgc gagttcacgc agcacgatat ccccggcctg gtgcccgtag gtgtcgttta
    20881 cgcgtttgaa atggtcgatg tcgagcagca ccagggatgc ggccatgcca tgccgtgccg
    20941 catacgccac ttccgcgcgc aagcgttcgt cgaagtggga gcggttgtag gccgacgtca
    21001 aagcatcgcg cgtgctcgac tcataaagtc gccgcatcag cgcttcttcg cgcagatcgc
    21061 gcaaggcgaa gcggaacgag acctgcggcc ccaacatgag ccacgtctcg tccgtcagta
    21121 gcttgtgcga gacgcgctct cccgccacga atgttccgtt gcgcgaaccg aggtcgtcga
    21181 ccacgaagcc ctccgcgccg cgcgagatct tggcgtgaaa gcgactgatg ctctcctcgt
    21241 cgatgcgcag ctggttggtg gggtggcgac cgatcccgaa cgggaactga tcgagcgcga
    21301 tcaccgagcc ggcgtggatg ccatccatgc ggatgagcag ggcgcgttcc gcgattggag
    21361 cgaccagcgg gtgatccgcg gtgcggagcg tcacctccat ggtgcgagcc tcctcctgga
    21421 attgaggctc tcgcatctcg gggacgctg
//
LOCUS       DASTKU010000709         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1922698, whole genome shotgun sequence.
ACCESSION   DASTKU010000709 DASTKU010000000
VERSION     DASTKU010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922698"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFQ61_24875"
     CDS             complement(<1..215)
                     /locus_tag="VFQ61_24875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9957765.1"
                     /translation="MNLATVAVKNLARNKFRALLTVLAVALSITFFLVLRTMLWSWTA
                     AMEQSASDRIGIRHKVTFIMTMPKKYI"
     gene            complement(330..992)
                     /locus_tag="VFQ61_24880"
     CDS             complement(330..992)
                     /locus_tag="VFQ61_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9957766.1"
                     /translation="MIRLQGVGKTFFRGKEPLEVLKSIDLDIPEGAYEALMGPSGSGK
                     STLLNLIAGLDQPSRGQLTVAGADVGKMSEGERAKWRAANIGFVFQTYNLMPVLTALE
                     NVELPLLLAKLPSSERRKRAEIALRVVGLEQRMDHLPRQLSGGQEQRVAIARAIVADP
                     KIIVADEPTGDLDRESANEILNLLEKLNKEFNKTIVMVTHDPAAAERAHLVRRLDKGN
                     LV"
     gene            complement(1034..2278)
                     /locus_tag="VFQ61_24885"
     CDS             complement(1034..2278)
                     /locus_tag="VFQ61_24885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9957767.1"
                     /translation="MPDSLSTDLAALRIDRTQRPPSAGGPLKTALIVLAIVGVGAVGY
                     TVGKPYAESKLFKTEVAVTEIATVSPAQASVELTATGYVVAQSVSKVSVKVGGKIAKV
                     YVKQSDTVKAGDVLFEIDPRDHEASIASARSQAAAARARAVAARAGLVETEIQAKRAL
                     NLAAEGVAPKSNAEDLAARVTSLKEQANASEAEARASTALVSALEINKGNFTVRAPIS
                     GVVVNRPPEVGEFVGPQPAGLAVDMGGVEISDFNTLMVETDVPEQRLALVKVGRPAEI
                     VLDAYPDRRYRGKVAEITPKIDRAKATVLIKVAFVDEKTGVLPNMAARVSFLTAELDA
                     EAIKAKPKTIVPASAIVSREGSKSVFVIDEGKVHLTPVALGPSFGSGFELARGPASGT
                     RLVKDPPANLADGQPIRERTQE"
BASE COUNT          468 a          861 c          866 g          536 t
ORIGIN      
        1 tcgatgtact ttttcggcat ggtcatgatg aaggtgacct tgtgccggat gccgatgcga
       61 tcgctcgcgc tctgctccat ggcggccgtc cacgaccaaa gcatggttcg gagcaccaaa
      121 aagaacgtaa tcgagagcgc gaccgcgagc accgtgagca gcgcacggaa cttgttgcgg
      181 gccagatttt tgaccgcgac ggttgccaga ttcaaagcgc gatcctccta aacgaccgaa
      241 aacgtgagcg acgggggcgg actgccggtg agctcgagag ccgcagtgtg agcgacccag
      301 atgattgtgt gaaccagagc gaggcagcct cagaccaggt tgcccttgtc gaggcgccgg
      361 acgaggtgag cacgttcggc cgcggccgga tcgtgcgtca ccatcacgat cgtcttgttg
      421 aattctttgt tgagcttctc gagcaggttc aaaatctcgt tggcgctctc gcggtcgaga
      481 tcacccgtgg gctcgtccgc cacgatgatc ttggggtcgg ccacgatcgc gcgggcgatt
      541 gcgacgcgct gctcctgacc acccgagagc tgccgcggca agtgatccat acgttgctcg
      601 aggccgacga cgcgtagcgc gatctccgcg cgtttgcggc gttcggacga gggtagcttg
      661 gcgagcaaca aaggtagctc gacgttctcg agtgccgtga gcacgggcat caagttgtag
      721 gtctgaaaaa cgaacccgat gttggcggcg cgccatttgg ctcgctcgcc ttcgctcatc
      781 ttgccgacgt cggcgcccgc cacggtgagc tgcccgcgcg agggttgatc caagcccgca
      841 atcaggttca agagcgtcga tttgccggag ccgctggggc ccatcagggc ctcgtacgcc
      901 ccctcgggaa tatcgagatc gatgctcttc aacacctcga gcggctcttt gcctcggaag
      961 aacgtcttgc cgacgccttg caggcgaatc atcgcagaac ttccgttttg gctcttctcg
     1021 ctcatgtctg cattcactcc tgagtgcgtt cgcggatcgg ctgaccatcg gccaggttcg
     1081 cgggtgggtc tttcacgagg cgcgtccccg atgcggggcc acgagccagc tcgaacccgc
     1141 tgccgaagga cgggcccaaa gcgacgggag tcagatgcac tttgccctcg tcgatcacga
     1201 agacggattt cgacccctcg cggctcacga tggccgatgc cggcacgatg gtcttgggct
     1261 tggctttgat ggcttcggcg tcgagctctg cggtcaggaa gctgacgcgg gccgccatgt
     1321 tcggcagcac gccggtcttc tcgtccacga acgcgacctt gatcagcacg gtggctttgg
     1381 cgcggtcgat cttgggcgtg atttccgcga cttttccgcg gtaccgccga tcgggatagg
     1441 catccaacac gatctccgcg gggcggccga ccttcacgag ggccaggcgc tgttccggga
     1501 cgtcggtctc caccatcaac gtgttgaagt ccgaaatttc gacgccgccc atgtccaccg
     1561 cgagccccgc gggttggggc ccgacgaact caccaacttc cggtgggcga ttcacgacca
     1621 cgcccgagat gggcgcgcgc accgtgaagt tgcctttgtt gatttctagg gccgaaacca
     1681 gagctgtcga tgcgcgtgcc tcggcctccg aggcgttggc ctgctccttc aacgaggtca
     1741 cccgcgccgc caaatcttct gcgttcgact tgggcgccac accttcggcg gccaggttca
     1801 gggcacgctt ggcttgaatc tcggtctcca ccagcccagc tcgcgccgcg accgcgcgcg
     1861 cccgcgccgc cgccgcttgg cttcgggcgc tcgcaatgct cgcttcgtga tcgcgcgggt
     1921 cgatctcgaa cagcacgtct ccggctttca cggtgtcgct ctgcttgacg taaacctttg
     1981 cgatcttgcc gccgaccttg acgctgacct tcgagacaga ttgagcaacc acgtaacccg
     2041 tcgcggtcag ctccaccgaa gcttgagccg gcgaaaccgt ggcgatctcc gtgacggcga
     2101 cttcggtttt gaagagcttg gattccgcgt agggtttgcc aaccgtgtag cccaccgccc
     2161 cgacgccgac gatcgccagc acgattaggg cagtcttcag ggggccaccg gcgctcggag
     2221 gccgctgggt ccggtcgatg cgcagcgccg caaggtcagt ggacagagag tccggcatcc
     2281 gattttcctc aaaagaaggg ccgcttcgct agcacagggg gccgtgcgcg tccccgtttt
     2341 cgatcgtccc aattcgggtg gtcgtggttt ccggcctgga ctggtctcaa taacacgggg
     2401 tcaaaggaaa cagagcgggc cgaactggtg gccccattgc acacgaaacc tccagcccgg
     2461 gcgaatcttg cacgcctggt cagctcgttc aatgccattg ggcggatctc gaaaagcgcc
     2521 agcaggggtt cgagcagcca acgggcagag ttttggtaca gtgtgcggcg tgcagttccc
     2581 gttgatcgcg caggtgagtg tcgtcgctgt tgcggcgatc ggagtattcg gcttcgtagc
     2641 aaccgcaaag gagggcgaga cacgccgcgc gtgtgcctcc ttttgcgccc tgcagcccaa
     2701 ctacgcgggg cgtaaccggc ttgcgccaga c
//
LOCUS       DASTKU010000710         5584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1027373, whole genome shotgun sequence.
ACCESSION   DASTKU010000710 DASTKU010000000
VERSION     DASTKU010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027373"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3131)
                     /locus_tag="VFQ61_24890"
     CDS             complement(<1..3131)
                     /locus_tag="VFQ61_24890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM system kinase/cyclase fusion protein"
                     /protein_id="HET9957768.1"
                     /translation="MASEIPEFQALWGEAYDIVQRLGRGGYATVFKAVQRATGQTVAI
                     KVQRLDPDQTAEERAARVARFERETRLCQTLTHPNIVRMIESGQSDRFLYIVFEFVPG
                     ETLSQLLIRRGALLAEESCWLMADVLEALAGAHSQGILHRDLKPDNIMVESRADQLLI
                     RVLDFGVGAFVPGTIEGAATLTRSHEILGTPCYAAPEQLRGEPATTRSDIYAWGLVFL
                     ECLTGQRVIDGATMAQVFQKQLSPLEVPLPPALAAHPLAALLRRALAKSPNERAGDAA
                     RLAAELRKVPIGDLVGLLTPHFNDRPARTLALDARKPVAEQRHVTALSFSLTVSAVAQ
                     DGRELGLDLETLDAVQRDQMSLCIDLVRRFGGHVAGTLANRMLAVFGYPEAMSGDARR
                     AGRAALEISSRLQVRRRLLASQRGIELEFRMGLHTGSVIVDPSGHAAGPALNLASRLE
                     GMAEAQTTLVTEATRQLLEPYLRFRPVHLDIPSRIYALVSEKASATLDVQQTGSDRGP
                     IIGRGAELEALNALLAQARAGSGALTLIRGEPGIGKSRLVQEVCRRALETGVGVLDGQ
                     CLLEDKNSALSPVLSLVRRYFELDEGLKEGLARIEELLPAARAREITSILCAWLELAK
                     PDGYPDPNLSAEAQRKLLLEALVRILSHASRSDAQLVVIEDVQWADPTTLELLRTLAR
                     EVKAQRTLVLLTARPEFTACPEEGSLIELSGLSPSDVERLLNERLGDLRLSRETRARI
                     VERTDGVPLFLDELSRSLLRAAQATEPSAEPDSSPLRQPNIPDSLRDLLSSRLDGLGQ
                     GKETAQLASAIGREFELELLSAISLRGTETLQGDLDSLVQSELVLRRATQDGARYVFR
                     HALIQEAAWDSMLPSQRRSVHRSIAQVLETKFADQAKQEPARLARHYELGHVPEKAVA
                     LRLQSAQQAARSLAYLESVAHARAGLALLPYVEDTALANTWDLELNNILGGMLSAAQG
                     FAAPELIEAFTRCQTLLQTTEPPPLQRLMTQKGLWTFHNARADYSSASLLVAQMLSLA
                     AAHPEPIFKLGAHT"
     gene            complement(3149..4069)
                     /locus_tag="VFQ61_24895"
     CDS             complement(3149..4069)
                     /locus_tag="VFQ61_24895"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9957769.1"
                     /translation="MGILRQRPEKRRVLLSGEFVVGRLASCSLVLDTPFVSTTHALIR
                     WQGDSWELRDLGSTNGTYIDGQRVAPGEAVRLSTGTLITFGHQSETWEVCDVTPPGPA
                     LVPMDGSEPLLLLDGVLAIHDGNGEVQGTVFYESSGWVLEVEHEKSPLLPGQTFTLAG
                     REYRFDCPIGLAATQASTERPWYLDETTLVLTVSSDEEHVQLELKTRGTAKDLGERAC
                     FYLALVLGRRRIEDRESQAAEHGWLPLESLLKMVPDYTNLSHLNLDIFRLRRLLGQAG
                     IQDAAGVIERRKGQLRLGTDRIELRRKRDG"
     gene            complement(4165..>5584)
                     /locus_tag="VFQ61_24900"
     CDS             complement(4165..>5584)
                     /locus_tag="VFQ61_24900"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9957770.1"
                     /translation="GMGAVYEGENTRIARRVAIKVLHSGVAEMGEAVARFEREAQAAG
                     RIGSEHICEVLDLGNLPSGDRYLVMEFMDGDSLSRRIRQRGQLTPSELYPIAHQLLDG
                     LMAAHAAGIIHRDLKPDNIFVLRSKRGGGEFVKLLDFGISKFTDGGSNLSMTRTGTVM
                     GTPYYMAPEQARGSRELDARVDLYAVGVILYEALTGQVPFMAETFNELLFKIVLETPR
                     PIGELVPGAEPSFVAIVEKAMSRDPALRFQSALEFQQALEKWASGGFQVTGSTLAVGQ
                     VPPMVSALQNTGNRGLSQSGGRASSPQLGTQTGPHGQQLGATSESWAGASAPNLQLPT
                     HPRRPLWIVGSVVGLLVVIGIGFAVRSGLKTDTPAAASSNVLGTTPPTAAPSSPPDIR
                     VEPAPSVPPPVVNADASSSSAEANSEPAPSASARPTAAPRASIAVRAPAPRPTSPPVA
                     PPVDPRKSAQPASTGRKIRTSL"
BASE COUNT         1044 a         1820 c         1739 g          981 t
ORIGIN      
        1 gtgtgggcgc ccaatttgaa gatgggctcc ggatgggccg cagccaagct aagcatttgc
       61 gcgactagta agctcgcgct cgaataatcc gctcgtgcgt tgtggaatgt ccacagcccc
      121 ttctgagtca tcagccgctg caacggcggt ggctccgtcg tttgtaaaag agtctgacag
      181 cgcgtgaatg cttcgatcag ctcgggtgcc gcgaacccct gcgccgcact caacatccca
      241 cctaggatat tgttgagttc gagatcccaa gtgttcgcca gggcggtatc ttcgacgtac
      301 ggcagcaacg cgaggccggc acgtgcgtgg gcaaccgatt ctaggtaggc gagagatcgg
      361 gccgcctgct gcgcagattg cagacgcagc gccaccgcct tctcgggaac gtgccccaat
      421 tcgtaatgac gagcgagccg tgccggctcc tgcttcgcct gatcggcgaa cttcgtctcg
      481 agcacctgcg caatgcttcg atgcacactg cgccgttgag acggcaacat gctgtcccag
      541 gcggcttcct ggatcaacgc gtggcgaaac acgtagcgcg cgccgtcttg agtcgcgcgg
      601 cgcagcacga gttcggattg caccaggctg tccagatctc cctgcaaagt ctcggtgcca
      661 cgcaaggaga tcgcgctgag caactccagt tcgaactccc gcccgatcgc ggaggccaac
      721 tgcgctgtct ctttgccttg gccgagccca tccaagcggc tactcaatag gtctcgcaag
      781 ctgtccggaa tgtttggctg acgaagcggg ctcgagtcgg gttcggcgct cggctccgtg
      841 gcctgggcgg cgcgcaacaa gctgcggctc aattcatcca agaacagagg tacaccgtcg
      901 gtgcgttcga cgatgcgagc gcgcgtctcg cggctcaagc gcaggtcccc caagcgctcg
      961 ttgagtagcc gctctacgtc actcggcgag agacccgaga gctcgatgag cgagccttct
     1021 tccgggcagg ccgtgaattc aggacgggcc gtgagcaaca ccagtgtgcg ctgcgccttc
     1081 acctctcgcg ccaaggtccg taagagctcg agcgtcgtcg ggtctgccca ttgtacgtcc
     1141 tcgatcacca ccagctgggc gtcactgcga ctggcatggc tcagaatccg aactagcgct
     1201 tcgagcaaca gttttcgctg tgcctctgcc gagaggttcg gatccggata gccgtcaggc
     1261 ttggccaact cgagccaggc gcacaggatg ctggtgatct cgcgcgcacg tgcggcgggc
     1321 agcagctctt cgatacgcgc cagcccttcc ttgagcccct catcgagttc gaagtagcgg
     1381 cgcaccagag atagcacggg ggacagcgcg ctgttcttgt cttcgagcag acattgtccg
     1441 tcgagcacgc ccaccccagt ttccaacgcg cgccgacaaa cttcctgaac cagacgcgac
     1501 tttccgatgc cgggctcgcc acgaatcaaa gtcagcgcgc cgctgccagc gcgcgcctgc
     1561 gcgagaagcg cgttcagcgc ctcgagctcg gcgccgcggc cgatgatggg gccgcgatcg
     1621 gagcccgtct gctgcacgtc cagagtggcg ctagcctttt cggagaccag cgcgtagatg
     1681 cggctcggta tatcgaggtg aacgggcctg aagcgcaggt acggctcgag caactgacgc
     1741 gtggcctctg tcaccaaggt agtctgcgcc tcggccatgc cttccaagcg cgacgcgagg
     1801 ttcagggcgg ggccggcggc atgcccagag gggtcgacga tcacgctccc ggtatgcagt
     1861 cccatgcgga actcgagctc gatgccgcgc tgggatgcga gcaagcggcg gcgcacctgc
     1921 agacgcgaag aaatctcgag ggccgcgcgc ccagcgcgac gagcgtcccc gctcatcgcc
     1981 tctggataac caaaaaccgc gagcatgcgg ttggcgagag taccggcgac gtggccaccg
     2041 aagcgccgca ccagatcgat acagagactc atttgatcgc gctgaaccgc gtccagcgtc
     2101 tccaagtcga gccccaactc gcgaccgtct tgggccaccg cggacacggt caggctgaag
     2161 gagagggcgg tgacgtgccg ctgctcggcc acgggcttgc gggcatcgag cgccaacgtt
     2221 cgcgccggtc gatcgttgaa atgcggtgtc agcaagccaa ccaagtcacc gatcggaact
     2281 tttctgagct ccgccgccag acgagccgca tcgcccgcgc gttcgttcgg cgatttggcg
     2341 agcgcgcgac ggagcagcgc cgccaaagga tgcgcggcca gcgctggcgg caggggcacc
     2401 tcgagcgggc tcaattgttt ctgaaatacc tgggccatgg tcgcgccatc gatcacgcgt
     2461 tggcccgtga ggcactctag aaacaccagc ccccaagcgt aaatgtcgga gcgggtcgtc
     2521 gccggctctc ctcgtaactg ctctggagcc gcgtaacacg gagtacccaa aatctcgtgg
     2581 ctgcgcgtga gcgtcgccgc gccttcgatg gtacctggca cgaaggcgcc aaccccgaaa
     2641 tccaagaccc gaatcaggag ttggtccgcg cgcgactcga ccatgatgtt gtcgggcttg
     2701 aggtcgcgat gcaatatacc ctgggagtga gcaccggcca acgcttcgag cacgtcggcc
     2761 atcagccaac agctctcttc cgcgagcaac gcaccgcgcc ggatcaagag ctggctcaac
     2821 gtctcgcccg gcacgaactc gaacacgatg tacaagaagc gatcgctttg accactctcg
     2881 atcatgcgta cgatgttcgg gtgcgtcagg gtctggcaaa gacgcgtctc gcgttcgaag
     2941 cgggccaccc gcgccgcgcg ttcttcagcg gtttgatcgg ggtcgagtcg ctggactttg
     3001 atggctacgg tctgacccgt ggcgcgctga acggctttga aaacggttgc atagccgccg
     3061 cggccgaggc gctgcacgat gtcgtacgct tcgccccata gggcctggaa ttcggggatc
     3121 tcacttgcca acgcacctgc tcccgacctc aaccgtcacg cttgcgtcgc agctcgatgc
     3181 gatcggtgcc gaggcgcagt tggcccttcc gacgctcgat cacgcccgcc gcgtcttgga
     3241 tgcccgcttg gcccaacaaa cgtcgcagac ggaaaatgtc caaattcaga tgagacaagt
     3301 tcgtgtagtc gggcaccatc ttgagcaagg actcgagcgg tagccagcca tgctcggcgg
     3361 cttgtgattc gcggtcctcg atccgtcgtc ggccgaggac caaagccaag tagaagcacg
     3421 cacgttcgcc caagtctttg gccgttcccc gggtcttgag ttcgagctgt acgtgttctt
     3481 cgtcactgga gacggtgagc acgagcgtgg tctcgtcgag gtaccacggc cgctccgtcg
     3541 aagcctgcgt cgccgcgagc ccgatcggac aatcgaagcg gtattcgcga cccgcaaggg
     3601 tgaaggtctg gccgggcagc agcgggctct tctcgtgctc cacttcgagc acccaccccg
     3661 aggactcgta gaataccgtg ccctgaactt cgccgttgcc gtcgtggatc gccaataccc
     3721 cgtccaggag gagcaatggt tcactcccat ccatcggcac caacgcgggc cccggcggcg
     3781 tgacgtcaca gacttcccat gtctctgact ggtgaccgaa cgtaatgagc gtgccggtgg
     3841 agagtcgcac ggcttcgccg ggagccacgc gctgcccgtc aatgtacgtg ccgttagtgc
     3901 taccgaggtc acgcagctcc cagctatccc cctgccagcg gatcagcgca tgggtggtgg
     3961 agacgaacgg cgtatccagc acgaggctac aagaagccag gcgacctacc acgaattcgc
     4021 cagacagcag tacccgcctc ttctcgggcc tctgtcgcaa aattcccatg gcgcgagact
     4081 acacaaaacc atcgccgctg tggatcccgc gcgcaagaac gcaccaccta atgacgacac
     4141 ctcggggtga ggccattttg caggtcacag actagtgcgg atttttcggc ctgtactcgc
     4201 cggctgcgcg gacttccttg ggtccactgg cggagctacc ggcggggatg tcggccgagg
     4261 tgctggagct ctaaccgcaa tggatgcgcg aggcgcggcc gttggtcgcg cggacgcgct
     4321 cggcgcgggc tcggagttgg cttcagccga ggagctcgag gcatctgcgt taactacggg
     4381 cggaggcact gaaggcgccg gttcaactct gatgtcgggc ggggacgacg gcgcggccgt
     4441 cgggggagta gtgccgagca cgttcgagct cgcggcggct ggagtgtccg tcttcagccc
     4501 gctgcgtacc gcaaagccaa tgccgatgac cacgagtaac ccgacgacgc tgcctacgat
     4561 ccaaagcggt cgacgggggt gcgttggaag ctgcaaattc ggcgcgctcg cgcccgccca
     4621 gctctccgaa gtcgcaccta gctgctgccc atgaggcccc gtctgcgtcc cgagctgggg
     4681 agaagaagcg cggcccccgc tctgcgacaa gccgcgattc cccgtgtttt gcagcgcgga
     4741 gaccatcggc ggaacctggc ccacggccaa ggtggaaccc gtaacttgaa agccaccgga
     4801 cgcccatttt tcgagcgcct gctggaactc gagcgccgac tgaaaacgca acgcaggatc
     4861 acgcgacatc gccttctcga caatcgcgac gaagctcggc tcggcgccgg gcacgagctc
     4921 tccaatcggg cgcggcgtct cgagcacgat cttgaagagc agctcgttga aggtctcggc
     4981 catgaagggg acctgtcccg tcaaggcttc gtacaagatc acgccgaccg catacaaatc
     5041 gacccgcgca tcgagttcac gcgatcctcg agcctgctcc ggcgccatgt agtacggcgt
     5101 tcccatcacc gtaccggtac gcgtcatgct caggttgcta ccaccatcgg tgaacttgga
     5161 gatgccgaag tcgagcagct tcacgaactc tccgccgccg cgcttggagc gcagcacgaa
     5221 gatgttgtcc ggcttcagat cgcgatgaat gatgccagcc gcgtgcgcgg ccatcagccc
     5281 atccagtaac tggtgcgcaa tcgggtagag ctcgctgggg gtgagctggc cacgctgacg
     5341 gatccggcgg ctcaggctat ccccgtccat gaactccatg acgagatagc gatcgcccga
     5401 cggcaggttg cctaaatcga gcacctcaca gatgtgttcg gagccaatgc gacctgcggc
     5461 ctgtgcttcc ctttcgaagc gcgcaactgc ctcgcccatc tcagcgacgc cagagtgcaa
     5521 aaccttgatc gccacgcgcc gggcaatacg agtgttctca ccttcgtaca cggcgcccat
     5581 gccg
//
LOCUS       DASTKU010000711         4314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1922759, whole genome shotgun sequence.
ACCESSION   DASTKU010000711 DASTKU010000000
VERSION     DASTKU010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1922759"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VFQ61_24905"
     CDS             <1..532
                     /locus_tag="VFQ61_24905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957771.1"
                     /translation="SVTQYVNHAVMTVFQGEQHAHRAMEACLALESHLRLRTFEARNA
                     TRPAADAATAAAIGVDSGLLIASPVGSLAHGCVSQTVLGSVVYNAARLASLANPNEIL
                     VSARARQNLPHPEDCELDSSRVLRDAEGTQPIFRVLRSGQSGGDTSPTTTVAGPHAEA
                     ASAEVANGQKPTGSGD"
     gene            complement(549..1520)
                     /locus_tag="VFQ61_24910"
     CDS             complement(549..1520)
                     /locus_tag="VFQ61_24910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957772.1"
                     /translation="MNKTKLYSVAEIQRALGGPEPNSPQPPSHEAVPDGERSSSSAAE
                     AGSHRVDPQYEVMAARPRLHLVPKSYAGAPEPVPSRSVLPEPFVLAPASRARPPALPD
                     FAEATPAGTSAFDLGTTRVLPVAELLARGKAIQQASAPPGASGSIPAPPQLSEVLAQA
                     GAHQAPQRAARQLEPLGACTRFALAKLGGARLSRRQRGLIVAAIGLLTVTGVSTGLAL
                     LSASPGPERATTRPSTASRPDRNYKLLSKAAQAAQRAAPLRGSANLPLKLAPGMTLER
                     AAADAGAEGRYQVAVELYEELFRLHPDKPVFREAARVLARRLKDASR"
     gene            complement(1523..2641)
                     /locus_tag="VFQ61_24915"
     CDS             complement(1523..2641)
                     /locus_tag="VFQ61_24915"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET9957773.1"
                     /translation="MIPHDVYTQTVLEFFGPVRSYLQDPSVTEVMINGPDQVHIERKG
                     RLELTDARFSSTESILCALRNVAQFAGKRFDEFNPILEARLPDGSRIEAVVPPAAPGG
                     PYVAIRRFSKESLNLEHLIQIGSLNEAAAETLSVLVQAKLNVIIAGGTGSGKTSLLNV
                     MTRFAPESERIIVIEDSKEVQVQRPHVVYLEARPPDERGRGAVSIRDLFRATLRMRPD
                     RIVIGEIRGAEALDIVQAMVSGHGGCLGTLHATYPRDTLTRLETMAMMSDVSLPLTAL
                     RLQIASGVNLVVQVTRAQDGTRKVSHITEVLGFDVTTNRYELRDIFVREYHGTDAKGR
                     IISELVPTGEIPSCRAQLIEHGLDLPPAIDEAARRRRS"
     gene            complement(2658..3248)
                     /locus_tag="VFQ61_24920"
     CDS             complement(2658..3248)
                     /locus_tag="VFQ61_24920"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9957774.1"
                     /translation="MKRAVLTGVLFLILGVGAGCTRTASVSPEDASSIRPGRDAEPVI
                     RRASQLLALGDYIRAEQYLNMALQRGADEKRVLPLLVSACIQDQRYRDAIQYLEQHLR
                     RHPTQQNARFVLAGLHMSIGQTALAKKELEQVIARDPEQADAHYALATLLRDENGNYS
                     AADSHFRAYLRLQPNGVHAEEAQAGLLVAIPESRTQ"
     gene            complement(3245..4126)
                     /locus_tag="VFQ61_24925"
     CDS             complement(3245..4126)
                     /locus_tag="VFQ61_24925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9957775.1"
                     /translation="MMEASALRFQLRQADGTQRELSVDADVARVGSGAHCEIRLPEVD
                     AAVEQLRIQAQPTGPLVEVRATRPPVLLNGATFQGGPLLPDAVLRLGSVELRVSTIDR
                     QVIVGLRPGPTPASRRAMYVLVGLGIPLGIALLALSPPPVSSGWSLPPQRLWDKEVPG
                     SCGETNPASAAALADKHWQEAEAAQERAPFSPQDGVQAVALFSQAAACYQTASEKEQA
                     ARASLRRGKLKQDLEQQFHVHQVRLERALVTQRYESVKTEIRILLSFVHHSGGEYANW
                     LSTLDRQIVFKSQDGAK"
     gene            complement(4123..>4314)
                     /locus_tag="VFQ61_24930"
     CDS             complement(4123..>4314)
                     /locus_tag="VFQ61_24930"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET9957776.1"
                     /translation="QAEEHGNPLARVLQIHAEVSRQQRSVRAEEAAAKAGAKMMLPTA
                     MVFGALLILIGTPLVRAMS"
BASE COUNT          809 a         1382 c         1362 g          761 t
ORIGIN      
        1 cagtgtgacc caatacgtca atcacgcagt gatgaccgtg tttcaaggcg agcaacacgc
       61 tcatcgagcg atggaggcct gcttggcatt ggaaagccac ctccgactac gcacgttcga
      121 agctcggaat gcgactcgac ccgcagcgga tgcggccacg gccgctgcca tcggtgtgga
      181 ctctgggctt ctgattgcga gtccagtcgg ttcactcgcc cacggctgcg tgagccaaac
      241 cgtgctcggc agcgtggttt acaatgccgc tcgcctggca agcttagcca atccaaacga
      301 gatcctcgtc tccgcgcgcg cgcggcagaa cttgccgcat cccgaagatt gtgagctcga
      361 ttcctctcgc gttctgcgcg acgctgaagg cactcagcca atctttcgag tcctgcgcag
      421 cggccaatcg ggtggtgaca catccccgac cacgaccgtg gcgggccctc acgccgaagc
      481 tgccagcgcc gaagtggcta acggccaaaa gccaacgggt tctggtgatt gagccgtgag
      541 gagatgcgct atctgctcgc atccttgaga cgtctggcca acactcgcgc cgcttcgcga
      601 aacaccggtt tatccggatg gagtcggaac aactcttcgt aaagctcgac cgcgacttgg
      661 tatctaccct cggcgcccgc gtcggccgcg gcgcgttcca aagtcatacc gggggcgagc
      721 ttgagcggca agttcgcgct cccgcgcagc ggcgcagctc tctgcgcggc ctgcgcggcc
      781 ttggagagca acttgtaatt gcgatcgggc cgactcgcgg tggagggtcg cgtcgttgct
      841 cgctcagggc cgggagaggc cgagagcaga gcgagaccag tggacacacc tgtcacggtc
      901 aagagcccaa tcgccgctac gatcagcccg cgttggcgcc tcgataaccg ggctccgccg
      961 agcttcgcta gcgcaaaccg ggtgcacgct ccaagcggtt cgagttgccg tgcggctcgc
     1021 tgcggcgcct ggtgcgctcc cgcttgcgcg aggacttctg aaagttgtgg cggggccggt
     1081 attgagccgg aggcacccgg cggggccgat gcttgctgaa tggccttccc gcgagcgagc
     1141 agctctgcaa caggaaggac gcgagtcgtg ccgagatcga aagcagaggt tccagctggt
     1201 gttgcctccg cgaaatccgg caaagcgggc gggcgtgcgc gtgaggccgg cgccagaaca
     1261 aagggctccg gcaggacgga acgcgacggc acgggttcgg gtgcgcccgc gtaagacttg
     1321 ggcaccaggt gcaagcgtgg ccgcgccgcc atcacctcgt attgaggatc aacccggtgg
     1381 ctgcccgctt cggcagccga agacgagctt cgctcgccat ccggaaccgc ttcatgcgac
     1441 ggtggttggg ggctattcgg ctccggccca cccagggcgc gctgaatctc agcaacgcta
     1501 tagagcttcg ttttgttcac gctcaagatc tccggcgtcg agcggcctca tcgatggcgg
     1561 gcggcaagtc caggccgtgc tcgatcagct gagctcggca cgaggggatt tctccggtgg
     1621 ggacgagttc gctgataata cgccccttgg cgtcggtacc gtggtattcg cgcacgaaga
     1681 tatccctgag ctcatatcga ttcgtggtca cgtcgaaacc cagcacctcc gtgatgtggc
     1741 tcaccttccg cgtcccatcc tgggcccgcg tgacctggac cacgaggttg accccggagg
     1801 cgatttgcag ccgcaaggcc gtgagcggca gactcacatc gctcatcatg gccatcgtct
     1861 cgaggcgagt cagggtatcg cgcggatagg tcgcgtgcag agttcccaga caaccgccgt
     1921 ggccgctcac catcgcttgc acgatatcga gcgcctccgc gccgcggatc tcaccaatca
     1981 cgatgcgatc gggacgcata cgcaacgtgg cgcgaaacag atcgcggata ctgaccgcac
     2041 cgcgaccgcg ttcatcggga gggcgcgcct ccaagtagac cacgtgcggg cgttgaacct
     2101 gcacctcttt ggagtcctca atcacgatga tacgttcgga ctccggcgcg aagcgcgtca
     2161 tcacgttcag cagcgaggtc ttgccgctgc cggtcccccc cgcgatgatg acgttgagtt
     2221 tggcctgcac gagcaccgac aatgtctctg cggcagcctc gttcaaagag ccgatctgga
     2281 tcaggtgttc gagattcaga ctctccttcg agaagcgccg aatggcgaca tagggcccgc
     2341 ccggtgcggc gggcggcacc accgcctcga tgcgcgaacc atccgggagc cgcgcctcca
     2401 ggatgggatt gaactcatcg aagcgcttgc ccgcgaactg ggccacattg cgcagcgcac
     2461 acaagatcga ctccgtgctg gagaaccgcg cgtccgtcaa ctcgaggcgg cctttgcgct
     2521 cgatgtgaac ttggtcggga ccattgatca tcacctccgt aacggaagga tcctgcagat
     2581 aagaccggac tggcccgaag aactcgagca ccgtctgagt gtaaacatca tgagggatca
     2641 tggcgctcca actaggttca ctgagtccga ctctcgggaa tggcgacgag cagcccggcc
     2701 tgcgcctcct cggcatggac accattgggc tgcaggcgca ggtaagcgcg gaagtggctg
     2761 tcggcggcgg agtaattccc attctcgtcg cgcaacagcg tagcgagcgc atagtgagcg
     2821 tccgcttgct ccgggtcccg cgcgatcacc tgctcgagct ccttcttggc cagtgcagtc
     2881 tgcccgatcg acatatgcag ccccgccagc acgaagcggg cattctgctg cgtaggatgc
     2941 cgacgcaagt gctgctccag atactgaatg gcgtcgcgat agcgctggtc ttggatacaa
     3001 gcactcacca gcaacggcaa gacgcgcttt tcatcggcgc cacgctgcag ggccatgttc
     3061 agatattgtt cggcgcggat gtagtcgccc aacgcgagca gctgagaggc gcgccgaatc
     3121 accggctccg cgtcacgtcc agggcgaatg gacgacgcat cctctggcga aacagaggcg
     3181 gtgcgcgtgc agccagcacc cactccgagg atcaggaaca atacacccgt gagtacagca
     3241 cgtttcattt ggcaccatcc tgcgacttga aaacgatctg acgatcgagc gtcgataacc
     3301 aattggcgta ttccccacct gagtgatgaa cgaaggagag aagaatacga atctccgtct
     3361 tgacgctctc gtagcgctgc gtgacgagag cgcgctccag gcgcacttga tgaacgtgga
     3421 attgctgctc gagatcctgc ttcagttttc cgcggcggag cgacgcgcgg gccgcctgct
     3481 ctttctcgga ggcggtctga tagcacgcgg ccgcttgaga aaagagcgcg acggcctgca
     3541 ctccgtcctg gggactgaag ggggctcgct cttgagccgc ttcggcttct tgccaatgct
     3601 tgtcggccag cgccgccgcc gacgcggggt tcgtctctcc acacgacccc gggacctcct
     3661 tgtcccaaag cctctggggt gggaggctcc accccgaaga gacgggcggc gggctcaacg
     3721 cgagcagcgc aattcccaaa ggaatgccga gcccaacaag cacatacatc gctcggcgac
     3781 tggcgggggt ggggcccggc cgcaaaccga cgatgacctg ccgatcgatc gtgctgacgc
     3841 ggagctcgac gctgccaagg cgcagaacgg cgtcgggaag caacggcccg ccttgaaagg
     3901 tggccccgtt cagcagaacg ggcgggcggg tcgcacggac ctcgacgagg ggcccggtcg
     3961 gttgcgcctg aatccgcagt tgctccacgg cggcatctac ctccggcaac cgaatctcac
     4021 agtgagcacc gctgccgacg cgagcaacgt ccgcgtcgac cgagagctcg cgctgtgtcc
     4081 cgtctgcttg ccggagctga aacctgagtg cgctagcttc catcacgaca tcgccctgac
     4141 taggggcgtc ccaatcagaa tgaggagggc gccaaacacc atcgcagtgg ggagcatcat
     4201 cttcgcacct gcctttgcgg ctgcttcttc ggcacgcacc gagcgctgtt gtcgggacac
     4261 ctccgcgtgt atttgcaaaa cacgcgccag cgggttgccg tgttcttcgg cttg
//
LOCUS       DASTKU010000712         9300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1028008, whole genome shotgun sequence.
ACCESSION   DASTKU010000712 DASTKU010000000
VERSION     DASTKU010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9300
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028008"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4884)
                     /locus_tag="VFQ61_24935"
     CDS             complement(<1..4884)
                     /locus_tag="VFQ61_24935"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9957777.1"
                     /translation="MIPSLFGCEILEVLHQGERSVVYRARQAGHAEACILKTARSDLA
                     DPVHARRLRREHELLTQLSDCEFVPRCSGLVFEGERLGLLLADSGARSLGQLGLAGGL
                     SREEFFALASKLVTALEQIHGRGVLHRDLHPGNVLLGSALGALQLIDFGSAALTKGGL
                     PSVPARGSLEGKLGYIAPEQTGRVNRSVDYRSDYYSLGVILFELLTGVLPFASRDPLE
                     LVHAHLARQPPAASALNPTVPPMLSQIVGKLLAKDADERYQSTAALRADLSRAQRDTG
                     AGRDGVEFELAASDRYHDFRFPQRLYGRERELEELCNAFDRVCAGQARVVIVTGPSGV
                     GKSRFVKEIASHVNARHGHFTSAKYEQYKHNVPFAALGEALDEVAKQILTESASNIAT
                     WRQQLLAAVSGTGRVLIELCPSFEAVLGTQPEVGALPPREAAQRLRQVMIGTARVLAT
                     AEHPLCVFMDDMQWAGAASMQVFKEAMLALRDAPLLLIAAVRDAEAGPTHPLWLTLEE
                     IEVAGVPVTRIPIHELSYDRCQQLIADALALSEAQSAELARLVHARTSGNPFFVRAFL
                     QSLHNDGLLDGLSNAGVSALEAIRRRGVTDNVVELLIDRVARLPAATQQVLRFAACLG
                     SQFELGTLTRVLGEPAGVELWPALVQELVLPLEDIASEAEFFAREANVRCRFAHDRIQ
                     QAIISTLSEGQRCELHLAVGRSLRLLAHHSPNLLFEASRHLNHALSALVDEGERRDLA
                     ALDLAAARESMRRSAGAEASNFALAGIAALGPTAFSREYELALDLHLTAAEAAFEWAD
                     HESLSSLVSQIVTHARSPLDSARARRLSGRAQQARGQSRDAIRTYLDALRLLGYELPE
                     SPTATQIQEELRDTARALRGREVEALLNLPECRSAEGELARELLSKLIFFAYAAVHEL
                     LPVAVCRLVRASVTEGNTAESANGYTFYGLLLSRDHDLDRAHRFGRVALELAHRYDNP
                     AVLSQTYLYANYLLMHWKLPLSALATEFRKAHQYGLVAGSPLNAACSATTLCICRFWS
                     GAELSSLAGDMLQYREQIQQFSLRLVLNWHEVLLQTVENLNIEGPAPSELLGPYYVER
                     ERLPQHSAASDKSSLFNYYVAKALLSYLFDQPVQALGCADAILPLVPLFATALWAVPV
                     AYLDALVRLARADELEGPEREPLLGSVEQSLRRLRGWASHNPADIACKVVALEAELSR
                     VRGDQEQARLLFRRAVELARNGGSALDEGITYELQGKHALRTGDQESARVALQSSREA
                     FRRWGAHAKVLHMEQRYAEVLRSSSASISFASFLNQTENVDVVDLLGVMHASRAISGE
                     IRLDRLLQRLMELLLESAGADVAYLLLERGGNWVIEAGRVADRGVVTTLESLDQSGLA
                     ARGLPEVAASIVNYVASTAETVLLGDAALSPRFGSDPRFRSELASGGARAASLLCFPL
                     RRMQTTRAIVYLENRLSREAFTARRIKALELLSSQAVISLENARLYDELEQKVEKRTR
                     ELLDKNAELGRALQQVTEMQRQLVTQEKLAALGALTAGIAHEIKNPLNFITNFADVSA
                     RLSEEVEQVLEPELDRIAAPVQSEVNAVLGELRVAVQKIAEHGARATAIINGMAAHAR
                     GGSGPRESTQLNTLLAQS"
     gene            complement(5003..5236)
                     /locus_tag="VFQ61_24940"
     CDS             complement(5003..5236)
                     /locus_tag="VFQ61_24940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957778.1"
                     /translation="MQDVFNFLTELAIDPFKTIDYASRPPEQRASIHEMLRVDGSAAS
                     GERIAGGHFEACRTCSDPGPDEPFPDFEDQVSS"
     gene            complement(5279..6643)
                     /locus_tag="VFQ61_24945"
     CDS             complement(5279..6643)
                     /locus_tag="VFQ61_24945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957779.1"
                     /translation="MSDGQVLRKLSKWSRTIEGIEKSGRLDQSGSLAKAYAQRGSLYS
                     EIGLAEAGLCDALRALLSHEGRRYGRAEAQLPDLMREYRSASERMAAIPATLWAQLGE
                     CFRVYTRDVLLSRSRRGAEQDVAAFIEYSLACFERASSALSHVGSSNPRQLGWVKAHH
                     AAVLTMKYWMSLSVDSPAEQAFLDAEELFRSAEQCYGEDSYDWAKRFRAFLYALRGRN
                     DERDQDYHHARMLLHELENTTASQQSSFDRSMAMLASYDVLDEKAAPNRIHAGRAGDV
                     PAGTAGAQQLREQAARESIERGFCAIRKDPEEFVAAFFAATSQWYLSKSAAKDEECER
                     FEATLGSMLDAAEIRANNAISQALMCLAGVQLLKALRSENSDEAKKPAKRAKSTLRLL
                     QRMQPDLETRAMLHRNLLWSKIQDDSSEEARRVREVFGEETFKKLKTLFSVSPGTASP
                     RDSE"
     gene            complement(6636..7601)
                     /locus_tag="VFQ61_24950"
     CDS             complement(6636..7601)
                     /locus_tag="VFQ61_24950"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9957780.1"
                     /translation="MDDRSANEHGRDEAGRNEPGCDQPGCEQPGSDQPGRGPRPAEAH
                     CSKPTAELAPHRGALVVVGTGIQWAAQTTLAARRAIEAADCVLFALADPWGARWVRSL
                     NPRAESFVYASDGGRREIYSRMVEDVLMALTRHQRVCAAFYGSPAILTQPAHDAIHRA
                     RAAGHRALMLPGVSSLDCLFADLAVDPGQGGLQFYEASEFLARPRQLDLGAHLVLGQI
                     ALVGERGVYDPRDEARLRAGLRRLAARLLQDYPPAHQVLLYEASQHPALEPRILAVPL
                     RDLYAIDVSEISSLYIPPIPREGDGLSRVKFEVFNSTQPGREAHV"
     gene            7851..>9300
                     /locus_tag="VFQ61_24955"
     CDS             7851..>9300
                     /locus_tag="VFQ61_24955"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9957781.1"
                     /translation="MRTIRRRYALEQEIGRGASGSVWTALDLELGRRVAIKLLRSDAL
                     ENAPARERFESEARTIALLRSPHVVQVYDAGVDEGEPFIVMELLHGESLEARLSRHPQ
                     LPFEIVAQLVSEIAKGLRAVHRAAVVHRDLKPANIFLCREPDREVVKILDFGISALLG
                     GTTAEKSALTFMGTPQYMSPEQYRGGVPEPPSDLWALGVVTYRMLIGRAPFDAASLPA
                     LQDLVEHATYRPASECRPELPSGIDAFFKRAFAPAPRDRFASASDLAAELASLKGSEH
                     GKPIRALFVDDESDMEILLKQRFRREVKSGKYEILFADSGEAALEELRRRPDIDVVLT
                     DLNMPGMHGLTLLERIPEVNPLVRVVVLSAYSDMANIRAAMNQGAFDFLGKPIDFGDL
                     TRTIDKSAANTHLIRQELASREENDVLRALIGRGLADRLVTLVKAADRERYTSFEGTV
                     VWVELPVPSDVFARANAAQLLELLNRRLDDIVP"
BASE COUNT         1733 a         3022 c         2872 g         1673 t
ORIGIN      
        1 gctctgcgcg agcagcgtgt tcaattgtgt cgattccctc ggcccgcttc caccccgcgc
       61 gtgtgcggcc atgccgttga tgatcgcagt ggctcgcgcc ccgtgctccg cgatcttctg
      121 gacggctacc cgcagctcac ccaataccgc gttgacttcg ctttgcaccg gcgcggcgat
      181 gcggtctagc tctggctcta gaacctgctc cacttcctcc gatagacgag ccgaaacatc
      241 cgcgaagttg gtgatgaagt tcagcggatt cttgatctcg tgggcaatgc cagccgtcaa
      301 agcgccaagc gccgcgagct tctcctgcgt aacgagctgg cgctgcatct ccgtgacttg
      361 ctgcagcgct cggcctagtt cggcgttctt gtccagcagc tcgcgcgtgc gcttttccac
      421 cttttgctcg agctcgtcgt acaaccgggc gttctcgagc gagatcacgg cctggctgga
      481 cagcagctcc aaagccttga tgcgccgagc ggtaaacgcc tcccgcgaga gtcggttctc
      541 caggtacacg atggcccgcg tggtttgcat gcgccgcaaa gggaagcaca gcaaagaagc
      601 ggcgcgcgcc ccgcccgagg cgagctcgct tcgaaagcgc ggatcggaac cgaatcgagg
      661 actcagcgcg gcgtccccga ggagcaccgt ttcggcggtg ctcgccacat agttgacgat
      721 cgaggctgcc acctctggca ggccccgcgc cgcgagccca gactgatcca gactctcgag
      781 cgtcgtgacg acaccgcggt ctgccacgcg accagcctcg ataacccagt tgccgcctcg
      841 ctcgagaagc aggtaagcca cgtcggcccc cgcgctctcg agcaacagtt ccatgaggcg
      901 ttgtaacaga cgatctagcc taatttcgcc cgagatcgcg cgcgaggcgt gcatcactcc
      961 gagtagatcg acgacgtcca cgttctcggt ttgattcaag aacgacgcga agctgatgga
     1021 agccgagctg gagcggagca cctccgcgta gcgttgctcc atgtgcagga ctttggcgtg
     1081 cgccccccag cgccgaaagg cctcccgcga gctctgcagc gcgacccgcg ccgattcctg
     1141 atcaccagtg cgcaaggcat gtttgccctg caattcgtag gtgatgcctt catccaaggc
     1201 cgaaccaccg ttgcgtgcca gttcgaccgc gcggcgaaac aacagacgag cttgctcctg
     1261 atcccctcgc acgcgcgaaa gctccgcctc tagcgccacg accttacatg caatgtcggc
     1321 ggggttgtgc gaggcccaac cgcgcaggcg tcgtaggctc tgttcgacgc tgcccagcag
     1381 cggttcacgc tcggggccct ccagctcgtc ggcgcgagcc agacgcacga gagcgtccaa
     1441 gtaggcgacc ggtaccgccc acagcgcggt ggcgaacaag ggcacgagcg gcagaatggc
     1501 gtccgcgcag ccgagcgcct gcacaggttg gtcgaacaaa tagctgagca gcgccttggc
     1561 tacgtaatag ttgaagagcg agcttttgtc cgaggccgcg ctgtgttgag gcaaacgctc
     1621 tcgctcgacg tagtaggggc cgagcagctc actcggagcc gggccctcga tattgaggtt
     1681 ctccaccgtt tgcagcaaca cctcgtgcca attcagcacg agtcgcaggc tgaactgctg
     1741 gatctgctcg cgatactgaa gcatgtcgcc ggccaggctg gacagctcgg cgcccgacca
     1801 aaatcggcag atgcagagtg tggtcgcgct gcacgcggcg ttcaaagggc tgcccgccac
     1861 caacccgtat tggtgcgctt tgcggaactc cgtggccagc gccgacagcg gcagcttcca
     1921 gtgcatcagc aggtagttgg cgtagagata ggtttgcgac aggaccgccg ggttgtcgta
     1981 gcgatgggcc agctcgaggg ccactctgcc aaagcggtgc gctcggtcca ggtcgtgatc
     2041 tcgagacagt agaaggccat agaacgtgta accgttggcg gactccgccg tgttgccctc
     2101 ggtcaccgag gcacgcacta gccggcacac ggccaccggc agcaactcat ggacggcggc
     2161 gtaggcaaag aatatcagct tgctcaagag ttctctggcg agctcaccct ccgcgctacg
     2221 acactctggc aagttcagga gcgcttcgac ctctcgcccc cgcaacgcac gcgccgtgtc
     2281 acgcagctcc tcctgaattt gggtggcggt cggcgactcg ggcagctcgt agcccaacag
     2341 gcgcagggcg tccaaatagg tgcgtatggc gtcccggctc tgacctcggg cctgctgcgc
     2401 tctcccgctc aagcgccgag cgcgggccga gtcgaggggc gagcgggcat gcgtgacaat
     2461 ctggctcacc aagctactca aagactcgtg atctgcccac tcgaaggcgg cctccgcagc
     2521 cgtcaaatga aggtctagcg cgagttcgta ctcccgtgaa aaggcggtcg ggccgagcgc
     2581 agcaatgccc gccagcgcga agttggaggc ttcggcgccc gcggatcgtc tcatcgactc
     2641 gcgtgcggcc gccagatcga gcgcggctag gtcacgccgc tccccctcgt ctaccaaagc
     2701 cgagagtgcg tgattcaagt gccgacttgc ctcgaacagc aggttcggcg agtgatgggc
     2761 caggagccgc agcgatcgcc ccaccgcgag atgcagctca caacgctgcc cttcgctcag
     2821 cgtcgagatg atcgcttgtt gaatacggtc gtgcgcgaag cggcaacgca cgttggcttc
     2881 ccttgcgaag aactctgcct cggaggcgat gtcctcgagc ggaaggacga gctcctgaac
     2941 caatgccggc cacagctcga ccccggccgg ctcacccagc actcgggtca gtgtcccgag
     3001 ttcgaactgg ctgcctaggc aggcggcgaa gcgcagcacc tgctgggtcg ccgcgggcag
     3061 acgcgcgacg cgatcgatca gcaactcgac cacgttgtcg gtcacgcctc gcctgcggat
     3121 ggcctcgagc gcgctcactc cggcgttgga cagcccatcg agtagcccgt cgttgtgtaa
     3181 cgactgcagg aaggcgcgca cgaagaaggg gtttccggaa gtgcgcgcgt gaaccaggcg
     3241 cgccaattcc gcgctctgcg cctcggagag cgcgagggca tccgcaatca gctgctggca
     3301 gcgatcgtag ctgagctcgt gaatcggaat tcgcgtaacc ggtacgcccg cgacctcgat
     3361 ctcctccagc gtcagccaga gtgggtgcgt gggcccggcc tcggcgtcgc ggacggcggc
     3421 gatcagcagc aaaggcgcgt cccgcaaggc caacattgct tctttgaaga cctgcatgga
     3481 ggccgcgccc gcccactgca tatcgtccat gaacacgcac agcgggtgct ccgcggtcgc
     3541 tagaacgcga gccgtgccga tcatgacctg gcgcaagcgt tgggcggcct cgcgtggcgg
     3601 aagcgccccg acttccggtt gcgtgccgag cacggcttcg aagctcgggc agagctcgat
     3661 caatacccga cctgtacccg agaccgcggc cagcagctgt tggcgccaag tcgcgatgtt
     3721 ggaggcgctc tcggtgagga tctgtttggc gacttcgtcg agcgcctcgc cgagcgccgc
     3781 gaatggcacg ttatgcttgt attgctcgta cttggcactc gtgaagtgcc cgtgacgggc
     3841 gttgacgtga ctcgcgatct ccttcacgaa acgagactta ccgaccccgg aagggcccgt
     3901 tacgatcacc actcgggcct ggccggcgca gactctgtcg aaggcattac acaactcttc
     3961 tagttcccgc tcgcgcccat acaggcgttg agggaaccgg aaatcgtgat accgatcgga
     4021 agccgcgagt tcgaactcga ccccatcgcg tccagccccc gtatcccgct gcgcccgcga
     4081 caagtctgcg cgaagcgccg ccgtcgactg ataacgttcg tccgcgtctt tggctagcag
     4141 cttgccgacg atctgggata gcatcggcgg gacggtggga ttgagcgcgc tcgccgcggg
     4201 aggctgtcga gccaagtgcg cgtgcacgag ctcgagcggg tcacgcgacg cgaacggcag
     4261 aacgccggtg agcagctcga acagaattac ccccaaagaa tagtaatctg atcgatagtc
     4321 gacgctgcga ttgacccggc ccgtctgctc gggcgcgatg tagccgagct tgccttccaa
     4381 ggaacctcgg gcgggcacgc tcggcaaccc gcccttggtg agagccgcgc taccgaagtc
     4441 gatcagctga agcgcgccga gcgccgaacc gagcagcaca tttccggggt gcagatcgcg
     4501 atgcagcacg ccccggccat ggatttgctc gagcgcggtg acgagcttcg aggcgagggc
     4561 gaagaactcc tcccgcgaga ggccgcccgc caagcccagc tgtccgagcg aacgggctcc
     4621 agagtcggcg agcagcaagc ccaagcgctc cccttcgaac accagccccg agcagcgcgg
     4681 aacgaactca caatcggaaa gctgagtcag cagctcgtgc tcgcgccgaa gccgccgcgc
     4741 gtgaaccgga tccgccaaat ccgatcgtgc cgttttgagg atgcaggcct ccgcgtggcc
     4801 agcctgacgg gcgcggtaaa ctacgctgcg ctcgccctgg tgcagaacct cgagtatctc
     4861 gcaaccgaat agtgacggga tcatcttcag cctagattcg tggccgacca aggacgtggc
     4921 cgagaccgac ctcggtaggt ctcgaatcct aggagtctat cctgcggctt ggaatgggct
     4981 accgcgccca cgcgcaatga ttctaggaag aaacttgatc ctcgaagtcc gggaagggct
     5041 cgtccgggcc tgggtccgag caggtacggc aagcttcgaa atggccgccg gcgatccgct
     5101 cgcccgacgc cgcagagccg tccacccgca acatttcatg tatcgaggcg cgctgctcgg
     5161 ggggacgaga agcgtagtcg atggtcttga acggatcgat cgcgagttca gtcagaaaat
     5221 tgaatacgtc ttgcatggtc cgtctccttg gctcgtgaat gctctccgcg aacccctttc
     5281 attcactatc gcgcggggat gcggttccgg gagagacact gaatagcgtc ttcaacttct
     5341 tgaacgtctc ctccccaaat acctcacgaa cgcgccgagc ttcttccgag ctgtcgtcct
     5401 gaatcttgga ccagagcaga ttacggtgca gcatggcacg cgtctccagg tccggctgca
     5461 tgcgctggag cagccggagt gtgctcttgg cgcgcttggc gggcttcttc gcttcgtccg
     5521 agttctcgct gcgcagagcc ttcaacagct gcacgcccgc gaggcacatc aaggcttggc
     5581 taatggcatt gttggcgcgt atttcggccg catccaacat cgatccgagc gtagcctcga
     5641 accgttcgca ctcctcgtcc ttggccgctg acttcgacaa ataccattgg ctggtcgccg
     5701 cgaagaaagc cgcgacgaac tcctccgggt cctttcggat agcgcagaat cctcgctcga
     5761 tggattcccg ggcggcttgc tctctcaact gttgcgcccc agccgttccg gccggcacat
     5821 cccccgcgcg gcccgcgtga atccgatttg gcgcggcctt ctcgtccaag acgtcgtagc
     5881 tcgcgagcat ggccatgcta cgatcgaaag aggactgctg ggaggcagtc gtgttctcga
     5941 gttcgtgcag caacatgcgc gcgtgatgat agtcttggtc tcgttcgtcg tttcggcccc
     6001 gcagcgcgta caagaaagcg cggaagcgct tcgcccaatc ataagaatct tcgccgtagc
     6061 actgttcggc ggaacggaag agctcctctg catccaaaaa ggcctgctca gccgggctat
     6121 cgacagagag gctcatccag tacttcatgg tgagcaccgc cgcgtggtga gccttgaccc
     6181 atcccaactg acgcgggttg gacgagccaa cgtggctcag cgccgagctc gcacgttcga
     6241 aacaagccaa gctgtactcg atgaaggccg cgacgtcctg ctccgctcct cggcgggatc
     6301 gactgagtaa gacgtctcga gtgtataccc tgaaacactc gcccagttgc gcccagagcg
     6361 tggccgggat cgcggccatt cgctccgacg cactccgata ctctcgcatg aggtccggga
     6421 gctgcgcttc ggcgcgtcca tagcgccgac cctcatggct gaggagtgcc cgtagcgcgt
     6481 cgcacagccc cgcttcagcc aacccgattt ccgagtacag gctcccccgc tgggcatatg
     6541 ctttcgccag gctaccgctc tgatcgagtc gacccgactt ctcgatccct tcgatggtcc
     6601 gcgaccactt gctcaatttt ctcaagacct gaccgtcaga catgggcttc cctcccgggc
     6661 tgcgttgaat tgaatacctc gaacttcacg cgtgataggc cgtcaccttc tcgcggaatg
     6721 ggcgggatgt ataaactcga tatctcgctg acatcgattg cgtaaaggtc gcgcaaaggt
     6781 accgcgagga tgcgcggctc cagtgcgggg tgctgcgagg cctcgtagag caacacctga
     6841 tgagcgggtg gatagtcctg aagcagccgt gcggcgagcc gccgaaggcc tgcccgcagc
     6901 ctggcttcgt ctcgagggtc gtacactcca cgttcaccca ccaacgcgat ttgccccagc
     6961 acgaggtgtg cgcccaaatc cagctggcgc ggccgagcga gaaactcgga ggcttcatag
     7021 aactggagtc cgccctgccc cggatctacg gccaagtccg cgaacaagca gtccagggag
     7081 gatactcccg gcagcatgag cgcgcgatgc ccggcggccc gggcgcggtg aattgcgtca
     7141 tgtgccggtt gcgtaaggat agccggacta ccatagaagg cggcacagac gcgctggtgc
     7201 ctagtcagcg ccatcaggac atcctcgacc atgcgtgagt aaatttcccg tcgcccgccg
     7261 tcgctcgcat atacgaacga ttccgcgcgc ggattcaggc ttcgaaccca gcgcgcaccc
     7321 cagggatcag ctaacgcgaa caagacgcaa tcagccgcct cgatcgcccg gcgtgccgcg
     7381 agcgtagtct gcgctgccca ctgaatgcct gttccgacca cgacgagcgc gcctcgatgt
     7441 ggcgcgagct ccgccgttgg cttggagcaa tgcgcttccg ccggccgtgg gccgcggcca
     7501 ggctgatcgc tgccaggttg ctcgcagcca ggctgatcgc agccaggctc gttgcgacca
     7561 gcctcatccc gaccatgctc gttggccgaa cgatcgtcca tgcgggacca acctaggtct
     7621 gtcgcccacg cttagaccat ttcaacccat gtcaggatcc cactctaagg tctttttcgg
     7681 cctggaagct ccagcctgac ggcgaccgcc gatggaacct gcgtacgaga cccgggcata
     7741 gcggaaatcg caagcgcgcg cggacaaatt gaccgacctg aaacgggttg ctaggtgaat
     7801 tgggctgccg catcactctc gggtagcaaa cggtttccaa gtaaaggcca atgaggacaa
     7861 tccggcggcg ctatgcgctc gagcaggaga ttggccgcgg tgctagcggc tcggtttgga
     7921 cggccctcga tctggagctg ggccgacgcg tcgcgatcaa gcttttgcgc agcgacgctt
     7981 tggagaacgc ccccgcgcgc gagcgcttcg agagcgaggc ccgaacgatc gctctgttgc
     8041 ggagcccaca tgtcgtccag gtttacgacg cgggcgtgga cgaaggcgaa ccgttcatcg
     8101 tcatggagct gctccatggc gagagcctcg aagcacgcct ttctcgccac ccgcagcttc
     8161 cgttcgaaat cgttgcgcag ctcgtgtcgg agatcgccaa gggattgcga gccgttcacc
     8221 gcgccgcggt agtgcatcgc gatttgaaac ccgcgaacat cttcctgtgt cgagagccgg
     8281 atcgtgaagt cgtaaagatt ctggacttcg ggatctcggc attgctgggc ggcacaaccg
     8341 cagagaagtc ggccctcacg ttcatgggga cgccgcaata catgagccct gaacagtatc
     8401 gaggtggcgt cccagagcca ccctcggatc tgtgggcgct cggcgtcgtc acctaccgca
     8461 tgctgattgg gcgcgcaccg ttcgacgcgg cgagcctgcc agccttgcaa gacctggtcg
     8521 aacacgccac ttaccgtccc gcctcggagt gtcgccccga actgccgagc gggattgatg
     8581 ccttcttcaa gcgcgcattc gccccagcgc cgcgggaccg ttttgcgagc gccagcgatc
     8641 tggcggcgga gctcgcgagc ttgaagggca gcgagcacgg aaagcccatt cgggcgctgt
     8701 tcgtcgacga cgaatcggat atggagattc tgctcaaaca gcgcttccgc cgcgaagtga
     8761 agagcggtaa atacgaaatc ttgtttgcag actcgggaga agctgcgctc gaggagctgc
     8821 ggcgtcggcc cgacatcgac gtggtgctca ccgacttgaa tatgccgggc atgcacggct
     8881 tgacgctgct cgagcggatt ccggaggtca atcccctagt gcgcgtggtc gtactctcgg
     8941 cctatagcga catggcgaat atacgcgccg ccatgaacca gggtgcgttc gactttctcg
     9001 ggaagccaat cgactttggg gatctgaccc gtaccatcga caaatccgcc gccaataccc
     9061 acttgattcg ccaggagctc gcgtcgcgcg aggagaacga cgtgctgcgc gcgctgatcg
     9121 gacgcgggct cgccgatcgc ctcgtcactc tagtgaaggc tgccgatcgc gagcgttaca
     9181 cctctttcga gggtacggtc gtctgggtcg aattaccggt tccatccgat gtgttcgcgc
     9241 gcgccaatgc ggctcagcta ctcgagctat tgaatcgacg gctcgatgac atcgttcccg
//
LOCUS       DASTKU010000713         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_137552, whole genome shotgun sequence.
ACCESSION   DASTKU010000713 DASTKU010000000
VERSION     DASTKU010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137552"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1033)
                     /locus_tag="VFQ61_24960"
     CDS             complement(161..1033)
                     /locus_tag="VFQ61_24960"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9957782.1"
                     /translation="MNRKYLEISNLEKSYPTPKGRTVIVRDFNLLVAEGEFVSIIGHS
                     GCGKSTVLSIIAGLNQSTGGGVLLAGREVREAGPDRGVVFQAPCLLHWLTALENVRLG
                     VDQVHTHKSKAERSQIAQHYLELVGLGDSLHKKPAELSSGMRQRVGLARAFALSPKLL
                     LLDEPFGMLDSLTRLELHEVLLQAWRKDKKTALMVTHDVDEALFLSDRIVMMTNGPEA
                     RVGEILTVPFERPRQRSVVMEHPEYYALRERLIGFLESEGKRKAEAAENVVPAAPLRT
                     PRWGFLRAFSGAAR"
     gene            complement(1030..1854)
                     /locus_tag="VFQ61_24965"
     CDS             complement(1030..1854)
                     /locus_tag="VFQ61_24965"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9957783.1"
                     /translation="MAILELKGVEKSYGKTHVLCDVNLSVEPGEFVAIVGFSGAGKST
                     LVSLLSGLQRPDKGTVSFKGQALSGPGPERGVVFQNYSLLPWLTAFENIYLAVDQVFP
                     SWSRSKKLEHTDRYVGLVGLSHARDRKPGELSGGMRQRVALARALSAEPEVLLLDEPL
                     SALDALTRATLQDELERIGRTSGKTIVMITNDVDEAVLLADRIVPLSAGPAATLGQSV
                     NVDIPRPRERVALNHDANFKRVRSILIDYLLGSKKKRSLPESPSIVPVAAALEPSA"
     gene            complement(1862..>2257)
                     /locus_tag="VFQ61_24970"
     CDS             complement(1862..>2257)
                     /locus_tag="VFQ61_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9957784.1"
                     /translation="KPDPAGTFTIAMCSMWPTVLNTALGVRSVPQDYLNVARVLKLSP
                     LKTLVKVMLPSALPFMFTGFRLSLGIAWLVIVAAEMLTGAPGVGGFLWQEYNALIYEH
                     IILCIVTIGVVGFVLDRLMGLVERRFRTA"
BASE COUNT          445 a          725 c          673 g          414 t
ORIGIN      
        1 ttttcgtagc tcggtctgcc cgagctcttc gtgttttcgg agctcggctt gtccgagctc
       61 tttgcgctcg tactgtcttc gcttactttg cttagctacg gtctaactgc ccgcggcagg
      121 ccctcgactg cgcggctttg ctcgaagggt tgactgaagg ttaacgtgca gcgccggaga
      181 aagctcgaag aaaaccccag cgtggcgttc gcagaggggc cgcaggtacg acattctcgg
      241 cggcctcggc tttccgcttg ccctcactct ccaagaaccc gatcaggcgc tcacggagcg
      301 cgtagtactc gggatgctcc atcaccacgc tgcgctgccg agggcgctcg aacggcacag
      361 tgagaatttc acctacacgt gcttcggggc cattcgtcat catgacgatg cgatcggaaa
      421 ggaacagcgc ctcgtccacg tcgtgggtca ccatcaacgc cgtcttcttg tccttgcgcc
      481 aagcctgcag cagcacctcg tgaagctcga gacgagtgag tgagtccaac atgccgaacg
      541 gctcgtcgag tagcagcagc ttgggagaga gcgcgaacgc gcgagccaag cccacccgtt
      601 gccgcatgcc tgacgaaagc tcggctggct tcttgtgcag agagtctccc aaacccacga
      661 gctccaggta gtgctgggcg atctggctcc gctccgcctt ggatttgtgc gtatgcactt
      721 gatccacacc gaggcggacg ttctcgagag cggtgagcca atgcaggaga catggcgctt
      781 ggaacacaac tccgcgatcg ggcccagcct ccctcacctc ccgccctgcg agcagcacgc
      841 ctccgcccgt cgactggttg agccccgcga tgatcgagag caccgtcgac ttgccgcagc
      901 ccgagtgtcc gatgatactg acgaactctc cctcggcaac gagtaggttg aagtcccgaa
      961 cgatgacggt tcggcccttg ggcgtcggat aggatttctc cagattcgat atttcgagat
     1021 acttgcggtt catgcggacg gctccagggc ggcggcgacg ggtacgatgc tgggcgattc
     1081 gggaagggag cgcttcttct tcgatccgag caagtagtcg atcaggatcg agcgcacgcg
     1141 cttgaagtta gcgtcgtgat tgagggccac ccgctcacgc ggacggggaa tatcgacgtt
     1201 tacggattgg cccaacgtag cggcggggcc ggcgctgaga ggaacgatcc gatcggccag
     1261 cagcacggcc tcgtccacgt cattggtgat catcacgatg gtcttgccgg aagtgcgacc
     1321 gatgcgctcg agctcatcct gcagggtggc gcgcgtcagt gcgtccagag cgctgagggg
     1381 ctcatccaac agcaagacct ccggttccgc ggagagagcg cgggcgagcg ccacgcgttg
     1441 gcgcatgcca ccactcagct cgcccggttt gcgatcgcgc gcgtggctca ggcccacgag
     1501 ccccacataa cgatccgtgt gttcgagctt cttgctgcgc gaccaggacg gaaacacctg
     1561 gtccacggcc aagtagatgt tctcgaacgc ggtcagccac ggcagcaacg aataattctg
     1621 gaacaccaca ccgcgctcgg gccccggccc gctcagcgct tggcctttga acgacacggt
     1681 gcccttgtct ggccgctgca ggccggacaa gagggagacc agagtcgact tgcccgcccc
     1741 cgagaaaccc acgatggcga cgaactcacc cggctccaca ctgagattca cgtcgcacaa
     1801 cacatgggtc ttaccgtacg acttttcgac acctttgagc tccagaattg ccatgatttc
     1861 ctcacgcggt gcggaagcga cgctcgacca agcccatcag gcgatccaac acgaacccaa
     1921 ccacgccgat ggttacgatg cacagaatga tgtgctcgta gatcaacgca ttgtactcct
     1981 gccacaagaa gccccccacg ccgggcgctc ccgtcaacat ctcggccgca acgatcacga
     2041 gccacgcgat cccgaggctc aggcggaacc ccgtgaacat gaatggcagc gctgaaggca
     2101 gcatgacctt cactagcgtc ttgagcggcg agagcttcag cacccgggcg acgttcaagt
     2161 agtcttgcgg caccgaacgc acgccgagcg cggtattcag caccgtgggc cacatcgagc
     2221 acatcgcgat cgtaaaggtg ccggccggat ctggctt
//
LOCUS       DASTKU010000714         7840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1253640, whole genome shotgun sequence.
ACCESSION   DASTKU010000714 DASTKU010000000
VERSION     DASTKU010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253640"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            532..837
                     /locus_tag="VFQ61_24975"
     CDS             532..837
                     /locus_tag="VFQ61_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HET9957785.1"
                     /translation="MATHITDDCINCGACEPECPNNAISQGDDIYVIDPELCTECVGF
                     YDHEACQAVCPVECCLPDPRHVETEQTLVQRAVALHPDDAELKKRVVSLTFPSRFRK"
     gene            complement(1002..1553)
                     /locus_tag="VFQ61_24980"
     CDS             complement(1002..1553)
                     /locus_tag="VFQ61_24980"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET9957786.1"
                     /translation="MIVPGSKLPEFSTTACVSVEKGKEFANITNKSYPGKWLVIYTYP
                     KDFTFVCPTEIVEFDKKLKAFEDRDAVVLGGSTDNEFSHLAWRQAHADLKVIKHALLF
                     FNPSLAQGLGILHPEAGVALRATIIVDPEGVVRFASSNDLSVGRNVDEVLRVLDGLQT
                     DELCPCNWKKGEETLTEKLKKSA"
     gene            2087..3094
                     /locus_tag="VFQ61_24985"
     CDS             2087..3094
                     /locus_tag="VFQ61_24985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957787.1"
                     /translation="MRFFGPGVTCAIGLGLWGCSSSTASYTSDADRGSTHAWISVERS
                     ETLSIGAASPTGAASIAVGASGASPASPGSPSNASPALPNPGALAPSANPAVAPQVRA
                     FASFMRTPPHVDAAAVARATGFELALPALGECTEGEPAREKAGAIAGGRAELLDVGEV
                     SVETQGTRVALALRAFPAVTDAISGVVYSTRDRMATLPAAELYSFGARGSSSLPGFVV
                     SAEAPAPLEGLSLNGSPISAESVLGREPAELRWKPGTSRDRLYVTMTTGSETIATCAF
                     RDDLGRGMLPALTSTSGRVNVAVHRLRSVPFNESVNGGVDEGELRFDFELAVDLPIDP
                     S"
     gene            3112..4245
                     /locus_tag="VFQ61_24990"
     CDS             3112..4245
                     /locus_tag="VFQ61_24990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957788.1"
                     /translation="MRGPHRVYFVPGMFGFGQLAGYDYFTHVRTALEQRYRAAGVQVM
                     FEDVPAPPTSSLRYRSRVLASTIARSAGPESGPIHLVGHSTGGLDVRLLLSPSVNLGL
                     PPEVLAFRSRVQTAIAVNSPHYGTPLASYFTTVSGTRLLYAVSLFTVVSLSLGGAPLA
                     VFSKLLGGVGSIDNLFGGELKLFSRFTDSILRFVDRNGRREIHEFLSKVRIDQGGVIQ
                     IMPEAMDLFNAATEDHVNVRYACVASAAPPPSSLRVARRVRSPYAAFTAALFSTMYQF
                     ASQPPNVYPYARPSTRQAELFRAGIDHEVDETSNDGIVPTLSMLWGELIWAGEADHLD
                     VLGHFHDDLTPRTHTDWVTSGSRFTRQRFAALMDAIAHFQLSS"
     gene            4459..4956
                     /gene="lspA"
                     /locus_tag="VFQ61_24995"
     CDS             4459..4956
                     /gene="lspA"
                     /locus_tag="VFQ61_24995"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET9957789.1"
                     /translation="MSVNYSRFGWLLFVGLLLGVVGCDHTTKHLALSELKNGADVSLV
                     KNVLSLEYVTNTDTAFGVLGSVFSAETRRVLILILQGLTIVFLGGWLVRRFPVISNIE
                     RAAFALVLGGAVGNFSDRLLRGHVIDFIHLRHWPVFNVADIAVCIGAGLLFLASRKHP
                     TRATS"
     gene            5294..7039
                     /locus_tag="VFQ61_25000"
     CDS             5294..7039
                     /locus_tag="VFQ61_25000"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET9957790.1"
                     /translation="MRATDSRRAACILICWLARAALGCSSQVEEPASHGGASGDSTAT
                     GAQAAGGSAQSAAGGTSSAGAARGGSSGAAGSGGGTPTGAQAKAWLTAARRSSRAELD
                     NVLRDVLGDTTAPASRFLVEDDYAPYDNDYTLQSASQALIDGLNALAEDVARRALSER
                     RSKLVPCTPTGPGDAVCFEQVVKSLGERLLRRPLLDDEVRAYLGLQAFATETNPAVAH
                     DFYTAVGLFLRAVLQDPEFLYRVEATSSSATPRLSGNEIATRLAFLIWGSTPDDDLLA
                     RAASGELDAAAGRAAAARRLLEDPRARGQLHRYHAMWLGYRAIPQSAALARAFQTETG
                     ALIDRVVFEERRNYLDLFTLDETYVDASLASHYGLAPPAAASSWVSYGSTGRAGILSH
                     GSVLSAFSKFSDTSPTQRGILVRTRLLCEDVAPPPPSVNVDQPPAAGDSKCKTDRYAR
                     HRSTASCAACHSLIDPIGMGLERFDMAGRYREHDDELPECLLDGKGELPGVGSFSGPG
                     ELGAKLVQSGSLESCSIRQYLTFATGHRPGAAESDLVQAELAAFRSRGRDLSAWIVDF
                     VSREAFVARQERETP"
     gene            7036..>7840
                     /locus_tag="VFQ61_25005"
     CDS             7036..>7840
                     /locus_tag="VFQ61_25005"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9957791.1"
                     /translation="MKLDRRTFLRGLGGASIGLPLLDCMLQRSSVARAQAAAPARRYA
                     IVFAGQALGGDEWEHDRFMLDGVRKQESGHYIVPAQTGSDYAITTPLKPLTALRDDFS
                     LVSGLAIPYSKTSAEAAAVPAGGAFRDFHGGGAGPLLCGTRSESANFTCRSITSDQVI
                     AKLNQGKTKQPSLVLRAQPSWYLSGSSFAGRQYISYGEGGTRIEAQTSPQIAFRSLFT
                     GFAPADSQARAEFEFNQRAQKSVLDLIEAKRKGLLAKVGTSDRLRLEQHF"
BASE COUNT         1324 a         2442 c         2585 g         1489 t
ORIGIN      
        1 agccatcggg gcaagtgccg ggctcgcaat tgaaaacagt gcaatagccg ccgggctgcg
       61 tgatgtcgca gagccgttcg cccgtcgcgg agcagttcac ggaagactca cacgagtccc
      121 caatttgcgg acggcacgcg acggccgaga gcgcgattgc aaaggccaat gtcgtccacg
      181 ccttcgaagt gccccctctg ccgctggcca aacttcgcag ccacgccggt accccagaac
      241 cgagcgaccg ccggaagccg acgcggcgga aggtgtgcgg ccgggccacg cccgagtaag
      301 tgcccggcga ttccgagtgg gtggacggcg ctgacccagc gcatgtcgct gacgccggct
      361 gggctaaaga ctgagactgg acggggatca tattgaagcg gcgggactct acaccaggac
      421 tcccggagca ccagtccttc gatcatgacc tcggccagaa tgacgcgaaa actcgctcac
      481 caggggtcgc aagtgccggg gcagtgagct aacactacca gcccaagacc tatggccacg
      541 cacatcaccg acgattgcat caattgtggc gcctgcgaac cagagtgccc caataacgcc
      601 atttcgcagg gcgacgatat ttacgtcatc gatcctgagt tatgcaccga gtgcgtcggc
      661 ttttacgacc acgaagcctg ccaggcggta tgccccgtag aatgctgctt gcccgacccg
      721 cggcacgtag aaaccgagca aaccttggtg caacgcgcgg tcgccttgca cccagatgac
      781 gcagagttga agaaacgagt cgtctcgttg accttcccct cgcgttttcg gaagtaaagc
      841 gagctagtta cgcacgacga cccggtgtga ggctggagcc tcagaccggg tcgctgtttt
      901 tggtgctctc ctgcagccgt ctaggccggc cgccgtccgt gagcctggcg cccggagcgc
      961 ggcggagccg agggcccgct gcgctcccga ccagcactag ctcacgccga cttcttgagc
     1021 ttctcggtga gggtttcctc gcccttcttc cagttgcagg ggcagagttc gtccgtctgc
     1081 aaaccgtcga ggacgcggag tacctcgtcg acgttgcggc ccaccgagag atcgttggaa
     1141 gaagcgaagc gaacgacgcc ttccggatcc acgatgatgg tcgcgcggag cgccacgccc
     1201 gcttcggggt gaaggatgcc gagaccctgc gcgagggaag gattgaagaa cagcagggcg
     1261 tgcttgatga ccttcaggtc agcgtgcgcc tggcgccagg ccaagtgcga gaactcgttg
     1321 tcagtcgagc caccgagcac gaccgcgtcg cggtcctcga acgccttgag cttcttgtcg
     1381 aactccacga tttccgtggg gcacacgaag gtgaagtcct tcgggtaggt gtagatgacg
     1441 agccacttac cggggtagct cttgttcgtg atgttcgcga actctttgcc tttttcgacg
     1501 ctgacacaag ccgtggtgct gaactctgga agtttgctgc cggggacgat catggttgcc
     1561 tcgttcttgg acgtgttctc gagttccgaa actcgcgagc tcccggaact ctgtttgttt
     1621 gattttcgag ctcgttcctc gaccgccgtg aaggcttagc caaagatcga gccctgggct
     1681 ggccgccgag gaagctaacg ctgcccggcg acccctggaa tggttcgcga agtagacata
     1741 gtctaaaccc tctacctcgt caaacccgat tactgttcca atccgatgcg cttccggaag
     1801 cgaaaagcag cgcggaccgc gacctcgccg ggctcacggt gagcttcgtc aatccacgtt
     1861 tccccttcgt ctctcatctt ctctcctcac ctctggacgt tcccctgggt aacctggacg
     1921 ctaggcgagt gtccacgcgt gcgggcaacg cggcaacccg ctcccaatga ggaatcgggt
     1981 gtgtgggagg agcgggtggt gggccgactc ggtgactgtc gaaatccgag acggtccatg
     2041 ttgcgcggag cttgcccagc ggcttcgccc tgcatagagt tacgtcgtgc gtttcttcgg
     2101 accgggagtg acctgtgcga tcgggcttgg cctgtggggc tgctcgagct cgacggcttc
     2161 ctatacgtcg gacgcggacc ggggcagtac gcacgcgtgg attagcgtgg agcgcagcga
     2221 aacactgagc atcggcgcgg cgagccccac gggtgcggct tcgatcgcag tcggcgcctc
     2281 gggtgcttca cccgcatcgc ccggctcccc aagcaacgcc agtcctgcct tgcctaatcc
     2341 cggcgctctc gcacccagcg caaacccggc ggtagcgccg caagttcgcg ctttcgcgag
     2401 cttcatgcgc acgcctccac acgtggacgc ggcggcagtc gcgcgagcaa ctggattcga
     2461 gctcgcgctg ccagcactcg gtgagtgcac ggaaggcgag cccgcgcgcg agaaggccgg
     2521 agccatcgcg ggcgggcgcg cggaactctt ggatgttggg gaggtcagtg tcgaaacaca
     2581 aggaacgcgg gtcgcgctcg cgctacgcgc cttcccggcg gtgactgacg ccatctcggg
     2641 cgtggtctac tcgacccgag atcgcatggc gaccttgccc gcggccgagt tgtattcgtt
     2701 tggagcgcgc ggcagcagct ccctacccgg cttcgtagtg agcgccgagg cccctgcccc
     2761 gctcgaaggt ctgagcttga acggcagccc gattagcgcc gaaagcgttc tagggcgtga
     2821 gcccgcggag ttgcgctgga agcccggaac gagtcgggat cgactctacg ttactatgac
     2881 caccggctcg gagacgatcg cgacttgcgc cttccgggac gatctcggtc gcgggatgtt
     2941 gccggcgctc acgagtacga gcgggcgcgt gaacgtcgcg gtgcatcgcc ttcgtagtgt
     3001 gccattcaac gagagcgtga atggcggcgt agacgaggga gagctgcgct tcgacttcga
     3061 gttggcggtc gacctcccga tcgatccgag ctgagctctg cttcgttgac tatgcgcgga
     3121 ccgcatcgcg tttacttcgt cccgggcatg ttcgggttcg gccagctcgc tggctatgac
     3181 tacttcacgc atgtccgcac ggccctcgag cagcgctatc gagccgccgg ggtgcaagtg
     3241 atgttcgagg atgttccggc gccgccgacg tcttccttgc gttaccgttc gcgtgtgctc
     3301 gcgagcacga tcgcgcggag cgctggcccc gaaagcggac caatccactt ggtggggcac
     3361 tcgaccggcg gcctcgacgt gcgattgctg ctctcgccga gcgtgaactt gggcttgccg
     3421 ccggaagtgc tcgcgtttcg ttcccgcgtg cagaccgcga tcgccgtcaa ttcgccgcat
     3481 tacggcacac ctctcgcaag ctacttcacc accgtttctg gtacccgatt gctatacgcg
     3541 gtgagcttgt tcacggtggt ctcgctgtca ctcggcggcg cgccgctcgc ggtgttttcc
     3601 aagttgctgg gcggcgtagg cagcatcgac aacctgttcg ggggcgagct caagctcttc
     3661 agtcgcttca cggattcgat cctgcgcttc gtggatcgca acggtcgccg agagatccac
     3721 gagtttttga gcaaggtgcg catcgatcag ggtggcgtca tccaaatcat gccggaggcg
     3781 atggacctgt tcaacgccgc gaccgaagat cacgtcaacg tgcgctatgc gtgcgtggcc
     3841 tccgcggcac ctccaccgag ctcgctgcgc gtggcgcgcc gcgtgcgcag cccttacgcg
     3901 gcgttcaccg ccgcgctgtt ttccacgatg tatcagttcg cgagccagcc gccgaacgtg
     3961 tacccctacg cgcgccccag cacgcgacag gcagagctgt ttcgagccgg catcgaccac
     4021 gaagtcgacg agacctcgaa tgacgggatc gtgccgacgc tgagcatgct ttggggcgag
     4081 ctgatctggg ccggggaagc cgatcatctc gatgtcttgg gccactttca tgacgatttg
     4141 acgccgcgta cgcacaccga ctgggtgacg agtggttccc gcttcacgcg ccaacgcttc
     4201 gcggcgctga tggacgcgat tgctcacttc cagctctcta gctgatgagg ctgcgggtgg
     4261 accggtcgcg agctcgcgtg ttacggcggc cgagggcgcg gggagctcgg agcgcctccg
     4321 tgcggcgcgg caccgggttc gggacggccg ggaatcttcc tgctttcgca gctttccacg
     4381 ctggagtgac tcggggtcac ccgtgaagcg gccgttctgg tgcattcgtg gaatactggg
     4441 ctttcgatag gtgttgatgt gtccgtgaat tattcgcgct tcggttggtt gttgttcgtg
     4501 ggtctgttgc tcggcgtcgt cggctgcgac cacaccacca agcacttggc gctgtcggag
     4561 ttgaagaatg gcgccgacgt ctcgctggtg aagaacgtgc tgtcgctcga atacgtgacc
     4621 aacacggaca ccgcgttcgg agtgctgggc agcgtcttca gcgccgagac tcgccgagtg
     4681 ctgattctga ttttgcaggg gctgacgatc gtgttcttgg gaggctggtt ggtgcgccgc
     4741 ttcccggtga tcagcaacat cgagcgggct gcgttcgcgc tcgttctggg cggcgcggtc
     4801 ggcaacttca gtgaccgact gctacgcggg catgtcatcg acttcatcca cttgcgccac
     4861 tggcccgtgt tcaacgtcgc cgatatcgcg gtctgcatcg gggccgggct gttgttcctc
     4921 gcatcacgca agcacccgac tcgagcgacg agctgagccc tgccaatgaa tagagcgcgt
     4981 cgctacggcg ccggcgcgcg tcgttgcagt gcgccgcttg cgcggtatcg aggcgggatt
     5041 tgaaggctct cgattgcgct agggagtcag tttacgggct tcgcgcccgt agtttcgagc
     5101 ggtcgaggcc atgcgtggtg aaggcacccg ggacgcgcac cccgatgcgg ttcatcgact
     5161 cgtaaactcc gctgttcccg ctgatatcga gtttgagaca cagcgccgga ggtgccgcgc
     5221 ggtgcgacaa atccaactct cgaggtggat tcgaacctta ccgagattcg aacgccagct
     5281 atcttgaacg gtcatgcgtg cgacggattc tcggcgggcc gcttgcattc tgatctgttg
     5341 gctggctcgc gcggcgctcg gctgctcgag ccaagtcgaa gagcccgcgt cgcacggcgg
     5401 tgcttcgggc gactcgacgg ctacgggcgc gcaagcagcg ggcgggtcag cccagagcgc
     5461 tgccggtgga actagctcgg cgggagccgc gcgcggcggc agctccggcg cggcgggcag
     5521 cggtggcggg acgccgacgg gtgcccaagc caaggcttgg ttgacggcgg cgcgacgctc
     5581 gagccgagcg gagctggaca atgtgttgcg tgatgttctg ggggacacga ccgcgccggc
     5641 gagccgcttc ctcgtggagg atgactacgc tccgtacgac aacgattaca cgttgcagtc
     5701 ggcgtcccag gcgctgatcg atgggctcaa cgcgctggcc gaggatgtcg cgcgtcgtgc
     5761 actgagcgag cgccgctcca agctggtgcc ctgcacaccg acgggtccag gggatgcggt
     5821 ttgcttcgaa caagtggtga agagcctggg agaacggctg ctgcgtcggc cgctgctcga
     5881 cgacgaggtg cgagcgtatt tgggattgca ggctttcgcg accgagacca acccagcggt
     5941 ggcccacgac ttctacacgg cggtggggct attcttgcgg gcggtcctgc aagatcccga
     6001 gttcttgtat cgggtggaag cgacgtcgag ctccgcgacg cctcgcttga gtggcaacga
     6061 gatcgcgacg cgactcgcgt tcttgatttg gggcagcact ccagatgacg atctgttggc
     6121 gcgcgcggcg agcggcgagt tggatgccgc cgcgggccgc gccgccgcgg cacggcgttt
     6181 actcgaggat ccgcgcgctc gcggtcagct acatcgctat cacgcgatgt ggctcggcta
     6241 tcgcgcaatc ccgcagagcg cagcgttggc tcgagcgttt cagaccgaaa ccggcgcgct
     6301 catcgatcga gtggtgttcg aggagcgccg gaactacttg gacctcttca cgctcgacga
     6361 gacgtatgtg gacgcgagcc tggccagtca ttacggtttg gcgccccctg cggccgcttc
     6421 gtcctgggta agttatggca gcacggggcg cgctggaatt ctttcgcacg gcagcgtgct
     6481 ttcggcgttc tcgaagttct cggatacgag cccgacccag cgtggaatct tggtgcgcac
     6541 gcgcctgctg tgcgaggacg tcgcgccccc gccaccgagc gtcaacgtcg atcagccgcc
     6601 ggccgccggc gattccaagt gcaagacgga tcgctacgcc cggcatcgct cgacggcatc
     6661 gtgcgcggcg tgccactcct tgatcgatcc gatcggaatg gggctcgagc gcttcgacat
     6721 ggccggtcgc taccgcgagc acgacgacga actgccggag tgcttgctgg atggaaaagg
     6781 tgagctgccg ggtgtaggta gcttctccgg cccgggagaa ctgggagcca agttggtgca
     6841 gagcggctca ctcgagagct gcagcatccg gcaatacctg acgttcgcga ccggacaccg
     6901 accgggcgcc gccgaatcgg atctggtgca agccgagctc gcggcgttcc gaagccgagg
     6961 tcgcgatctg agcgcttgga tcgtggattt cgtgagtcgt gaggctttcg tagcgcgaca
     7021 ggagcgtgaa acgccatgaa gctagatcgt agaacctttt tgaggggcct cggcggcgca
     7081 agcattggtc tgcccctact ggattgcatg ctccagcgct cgtcggtggc gcgcgcgcaa
     7141 gcggctgcgc ccgcgcgacg ctacgcgatc gtattcgcgg gccaagcgct gggaggtgac
     7201 gagtgggagc acgatcgctt catgctcgat ggcgtgcgca aacaagaaag cggtcactac
     7261 atcgtcccgg cccagaccgg ctctgattat gcgatcacca cgcctctcaa accgctcacg
     7321 gccttacgcg atgatttctc gctggtctcg gggctcgcga ttccttacag caagacctcc
     7381 gcggaagcgg ccgcggtgcc agctggcggc gcgtttcgcg acttccatgg cggcggagcg
     7441 ggcccgctgc tgtgcggcac ccgatcggag agcgctaact tcacgtgccg cagcatcacc
     7501 tccgaccagg tgatcgccaa gctcaatcaa ggtaaaacca agcagccatc actggtgctt
     7561 cgcgcgcagc cgtcttggta tctgagcggc agtagtttcg ccgggcggca gtacatttcg
     7621 tatggcgaag gcggcacccg catcgaggcg caaaccagcc cgcaaatcgc ctttcgctct
     7681 ttgttcaccg gctttgcgcc cgcggacagc caagcgcggg ccgagttcga attcaatcaa
     7741 cgcgcgcaga agagcgtgct cgatctgatc gaggccaagc gcaagggcct attggcgaag
     7801 gtgggtacgt ccgaccgact gcgcctcgag caacacttcg
//
LOCUS       DASTKU010000715        11367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1477560, whole genome shotgun sequence.
ACCESSION   DASTKU010000715 DASTKU010000000
VERSION     DASTKU010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11367
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477560"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFQ61_25010"
     CDS             complement(<1..699)
                     /locus_tag="VFQ61_25010"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HET9957792.1"
                     /translation="MQRSAYWIGVGLLALACDAGRSTAAGPSTAPATGGVTSQVTLTV
                     SAANPQPVSSSFFGQNYWSWVTAWGDPVATIEPQAKELGIKLLRAGGANNDQQNPERF
                     NEAEIDGFVDYARAVGAEPMLQVPVLKGPTGAAATAQDAAELVRYVNVTRAYGIRYFS
                     IGNEPDLYTEQKFRDASFDASAACATFAEFAKAMKQVDPSILVLGPELSWKYRRGGND
                     WLTPFLQGCGTEIDI"
     gene            861..1328
                     /locus_tag="VFQ61_25015"
     CDS             861..1328
                     /locus_tag="VFQ61_25015"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET9957793.1"
                     /translation="MSEHKAWVEWQLADGDFSAGQYSREHTWRFDGGLSVPASPSPSV
                     VRVPYSNPAHVDPEEAFVASISSCHMLTFLYLASRRGFVVTRYADEAVGRMTKNERRV
                     PWVSSVELHPEIEYGTPRPTPEEETELHHAAHAECFISNSVKTDIRVAARSAP"
     gene            complement(1325..1627)
                     /locus_tag="VFQ61_25020"
     CDS             complement(1325..1627)
                     /locus_tag="VFQ61_25020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957794.1"
                     /translation="MAAVDQGVPAFLQKVQVEASVRDGKFVGWQVRGLFPPADWQGID
                     LQPGDVITAVNGKSIERETDAYDVFVSLKTAPRLEVSVLRGAEPRRLTFEIVPRPN"
     gene            1882..3033
                     /locus_tag="VFQ61_25025"
     CDS             1882..3033
                     /locus_tag="VFQ61_25025"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET9957795.1"
                     /translation="MSNAPGKSKAGQAPLAKPRLHPRRFSCHVRPVEPTMLINDPRTE
                     AVPRFLNPIARLLNDPRDVVFVALMLRCAAVAGFGLSLFFLGRWAIYLVPVYWLALFG
                     LVMDRFTLMLHCTSHRQLFNKTARPLNHVIPWLLGPFFGQTPNTYFAHHMGMHHREEN
                     LADDLSSTLPYQRDRLDHWLRYYLRFLFIGLPELGVYFARRKQWKFVGRIALGEGLYW
                     SMIAGLLWLHPVAAAAVFVVPLLAIRTAMMMGNWAQHSFVCAEHPEDPYRSSITCINT
                     RYNRRCFNDGYHILHHVKPRCHWSEHPDEFLRALPEYAQHDAIVFDGIDYFQIWVYLM
                     FGKYDRLAQHFVRLPGAPERSPREVEQFLKQRVRRGPEPRPNISAASVA"
     gene            3072..3917
                     /locus_tag="VFQ61_25030"
     CDS             3072..3917
                     /locus_tag="VFQ61_25030"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET9957796.1"
                     /translation="MSIRKQTLSAAGVRQALRAHADPERARALARFFKTGPGEYAAGD
                     TFWGIGVPPQRAIARQFAALPLDELLKLLQSPVHEQRLTALLILVRQFERADEHGRQE
                     RFRFYVRHLAYVNNWDLVDSSAHKIAGEYLLSRTRGERELLYRLARSKNLWRRRVSII
                     ASAAFINAGDARDTLALAEILLGDEHDLIHKAVGWMLREVGKRIDRKVLLEFLRQHAV
                     AMPRTALRYSIEHLPASERAAWLAMRAERAARPTMPRVRTRGASPRAKLQPEAELSKP
                     LLQKK"
     gene            4101..5861
                     /locus_tag="VFQ61_25035"
     CDS             4101..5861
                     /locus_tag="VFQ61_25035"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET9957797.1"
                     /translation="MSVSSQPSNRPTAGAGSPANAGASSSPSSAVAGSSAAGSAAANS
                     GGRGSVVPIPEDCSAETLGEPSPVRRLSRLEYVNSLRALLAEYGLERELDASRELLAQ
                     LPPDGENSHLFSEMDARTSARHVDAFYAVADDLATRLSRAPTLTRIAGECAAQSPVER
                     ACIQSFVDRFGRLAARRTLSAEQSRRAMELYDGSLTGAEIVRRLVFTFLLSPEFLYHL
                     ELEGLESSTSPRALELDGPALAQRLSFHFLRQPPSSAVLDAAEAGQLSTNAGYEALVD
                     ELFERSENRALVSRFFHEWFQLDGFAGFADTRAFQAFAAGTTPNGELYADMLKEVDAL
                     IHRFTFESAGTYADLLTTRLSLTSSSGLASLYGVAPWSGAGQPPSFAAEQRAGLLTRA
                     ALLVEGNEMTNPIKRGAFILKHVLCVPLEPPSNLPAEALALPPANAAQTTRTRFQNKT
                     APRECAGCHELINPLGFALEAYDALGRFRTEEQLFDDAGRAQATLPVDAHVTLSLGGT
                     RAEVSSPVAFSDALAQSAEAAACFARQYFRFSVRRDEGALDACTIARVQQSLRTGGLK
                     AAFRSLVFSEAFKHRVIEAP"
     gene            5858..7489
                     /locus_tag="VFQ61_25040"
     CDS             5858..7489
                     /locus_tag="VFQ61_25040"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9957798.1"
                     /translation="MKIDRISRRMFLQGVSGSLLALPFLPSLVARSAEAQSAAAPLRL
                     ITIKSYSAQNIRDFYPARAVPGYTLRGYGGDETGGANGKQDGTLALTQSLSANSGKHS
                     NGSVYTGKQAPLRDFASPGISRILGPALNPYLDKLLLLRGLDFLPDTNHNDGGILGNF
                     GGGAISDSAGSIREVPTIDQVLAYSPKFYSTAPPGGRSLHLSPGRQNTFSYTHAGKKG
                     GPVTQVQADTDPRLVFDRIFANVSAPAAEGVNPNSLLVNRVIEDYRRLRSHPRMSAED
                     RARMEQHIALMAELEARLQSKPGAACQAPPRPAALGNVGVNVMSLRQSYAAMLDILAA
                     AIRCDLTRLVTLDVYKAIGASAGEEQGFEHSCASCQGNPNPTDWHRAAHDWDQKPQRE
                     KVVSINEWIAKEVFAALLSRLDVPEGNDKTFLDNSLVLWGNELGMNHLNYSVPVVMAG
                     SAGGRLATGRYIDYIDWNQPVRFSQHDGMVIEGVPYNRLLVTILQALGLSPEDYETEP
                     NAGYGEITPLGKPATAFATDYDYTQIGQPLPTLLT"
     gene            complement(7523..9277)
                     /locus_tag="VFQ61_25045"
     CDS             complement(7523..9277)
                     /locus_tag="VFQ61_25045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957799.1"
                     /translation="MARQIWGRQALQTAIAGACWGGTAALPLIAAATWLRIPVHGWAA
                     LVGVAAGAVAVGAVAGAILGFKRRWNLSQVALFLDHRLATGELLVTALETEPRPSAVG
                     ALLRAQAERALQTVPRGTLSLRLFTPRQAFAVPVFAASVWLATHPPQRAQAPIVPGSA
                     RVKLDTVRGLDRVEALEQAPSLSNADAQRLRQLAEQAKKLRRDLAKGVEEREAQAELA
                     RLREELDGQRQRFSDRAERAGLEAALSELGAEPSTRKAEKALGDGDIIAFDEEMQRLA
                     TRAEARARDAAREALERAKQRAREQKAQKLADMLERQSSNFAKREQESAALRELAKQL
                     EGALSAEGKEALSELNGTGNPAAQRRLAEALAKALESLSDEERKRLAEALKQRLSGAA
                     PGAQALSREELSRMLKELETPEGREALARALRELAREDNLDAERERALGEAERGRAQA
                     ERGLSKLPLPMPGPGMASPGSDPGSKANTGAPSSENGGPASGQPGSGPGRGDHGRTKP
                     EAVRSEEFRARAEARWLPGESLAGRSFGRAPGRAGETANEVGMGSLLSRGNGEVGAVE
                     GGDVPEEYREQVGRYFEP"
     gene            9617..10522
                     /gene="panB"
                     /locus_tag="VFQ61_25050"
     CDS             9617..10522
                     /gene="panB"
                     /locus_tag="VFQ61_25050"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET9957800.1"
                     /translation="MHKNKITVPDLIARKAAGPKISVLTAYDATMARLFDDAGVDVLL
                     VGDSLGMVVQGQANTLGVTIEDICYHGRAVARGTRRAHLVGDLPFMSFQGSADKALES
                     AGRLLKEGSFEAVKLEGGARYAEHVRLLVESGIPTMGHLGLLPQSVHAMGGFRVQGRD
                     DASAERILRDARVLEEAGAYAIVLEGIPMDLARRVTESITIPTIGIGAGPHCDGQVLV
                     CYDFLGMYSDIRPKFVKRFAELGDCVAAAAQAYVSEVQSGAFPGIEHSFGAAPPRPRP
                     KTEETYPRLDPAAPATYGPASEESV"
     gene            10519..>11367
                     /gene="panC"
                     /locus_tag="VFQ61_25055"
     CDS             10519..>11367
                     /gene="panC"
                     /locus_tag="VFQ61_25055"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET9957801.1"
                     /translation="MKPQLIERAQDFRRRCDTVRASSRAVGLVLTMGALHRGHMALVQ
                     AAKARGLVPLVTIFVNPTQFGPNEDYARYPRTLERDLELCGEHGVESVFCPSVEEMYP
                     PGDRTRVRVSGLTQALCGPKRPGHFEGVATVVTKFFALAGPCVAFFGRKDYQQLQVIR
                     RFARDLLLPVEVKGEPTVREPDGLALSSRNSYLSADERACALVIPRALSAAVARHRAG
                     ERRAEPLRTSVQAALEAAGMRVDYVELATPEELEPISDASVVGERALLALAAFVGKTR
                     LIDNVVL"
BASE COUNT         1981 a         3830 c         3507 g         2049 t
ORIGIN      
        1 gatgtcgatc tcggtgccgc agccttgcag gaatggcgta agccagtcat tcccaccgcg
       61 ccgatacttc cacgacagct ctggcccgag gacgagaatg ctcgggtcca cttgcttcat
      121 ggccttggcg aactcggcga acgtggcaca cgcggcgctg gcgtcgaagc tcgcgtctcg
      181 aaacttctgc tcggtgtaga ggtcgggttc gttgccgatc gaaaaatagc ggatgccgta
      241 ggctcgcgtc acgttgacgt agcgcaccag ctctgccgca tcctgagcgg tggcggccgc
      301 gccggtcggc cccttcagca ctgggacttg cagcatcggc tcggcgccca cggcgcgcgc
      361 gtagtcaacg aacccgtcga tctcggcttc gttgaagcgc tctggattct gctggtcgtt
      421 gttcgcgcca ccggctcgca gaagcttgat tccgagctcc ttggcttggg gctcgatggt
      481 cgcgacgggg tctccccaag cggtgaccca gctccagtag ttctgcccaa agaagcttga
      541 agaaactggc tgtgggttcg cagcgctcac ggtcagagtc acctgggaag tgacaccgcc
      601 ggttgcaggc gcagtgcttg ggcctgccgc agtgctgcgc cccgcatcgc aggctagcgc
      661 gagtaaacca acgccaatcc aataggccga acgctgcatg gcgggagtat ggcgggaact
      721 cgcgcgcgtg gccgtaggga tccagcattc gagaccgcga ggcagccaag tgatgccaaa
      781 ggacaggagc cgggtaggac acctcgccag aagagcgcga gcttgcgccg aaacagatct
      841 ccgctactac gtcggcgacc atgtcggaac acaaggcttg ggtcgaatgg cagctggcgg
      901 acggggactt ttctgcgggc cagtattcga gggaacacac ctggcgcttc gacggcggcc
      961 tgagcgtccc ggcttcgccg tcacccagcg tcgtgcgcgt gccgtactcg aaccccgctc
     1021 acgtcgaccc ggaagaggcg ttcgtggcgt cgatttcgag ctgccacatg ctcacgttct
     1081 tgtatctggc ctcgcgccgc ggcttcgtgg tcactcgtta cgcggacgaa gcggtggggc
     1141 gaatgacgaa gaacgagcgg cgcgtgcctt gggtgagttc ggtcgagctc catcccgaga
     1201 tagaatacgg cacgcctcga cccacgccgg aggaggaaac ggagctccac cacgcggccc
     1261 acgccgagtg cttcatcagc aactccgtca agacggacat ccgcgtcgcg gcacgatccg
     1321 cgccctagtt cgggcgcggc acgatttcga aggttagccg gcggggctcg gcgccgcgga
     1381 gcacgctgac ttcgagccga ggcgcggtct tcagagagac gaagacgtcg tacgcatcgg
     1441 tctcacgctc gatggacttg ccgttgaccg cggtaatcac gtctcccggt tgcaagtcga
     1501 tgccctgcca atccgcgggc ggaaacagcc ctcgcacttg ccagcccacg aacttcccgt
     1561 cgcgaacact agcttcgacc tggaccttct gcagaaaggc gggaacgccc tgatcgacgg
     1621 cggccacgac atcctcccgg tacagcttgc ccgctggcct cgcccgaggg cgggacggcg
     1681 cgggagccgt cgccgggggt gccatcgcgg gagccgggcc ctccaaggcg ggggccgaac
     1741 cacacgcggt tagacaggtg agcaggctcc aaacccgcca ttgcagcccg atccgcctgg
     1801 aaaactgcgg ggttggctga tgactactcg aaaccagggc gctcacgtgc gcagtcgatt
     1861 tcacatccgg gactatgctc gatgagcaac gctccgggca aatccaaggc tggccaagcg
     1921 ccgctcgcga agccacgctt gcacccgcgc cggttcagtt gtcacgtccg gcccgtagaa
     1981 cccaccatgt tgatcaacga ccctcgcacg gaagccgtgc ctcgtttcct gaacccgatc
     2041 gctcgcctgc tgaatgaccc gcgggacgtc gtgttcgtgg cattgatgct gcgctgcgcg
     2101 gcggtcgcgg gcttcggatt gagtctgttt ttcctggggc gctgggccat ctatctggtc
     2161 cccgtctact ggctcgctct gttcggcctg gtcatggacc gtttcacttt gatgttgcac
     2221 tgcacgtccc atcggcagtt gttcaacaag acggctcggc ccctcaatca tgtcattccg
     2281 tggttgctcg ggccgttctt cggtcaaacg cccaacacct acttcgctca tcacatgggc
     2341 atgcaccacc gtgaagagaa cctggccgac gacctcagca gcaccctgcc ctaccagcgc
     2401 gatcggctcg accattggtt gcgctattac ctgcgctttc tgttcatcgg cttacccgag
     2461 ctcggcgtgt acttcgcgcg tcggaagcag tggaagttcg tagggcgcat cgcgctcggc
     2521 gaagggctgt attggagcat gatcgccggg ctgctgtggc tgcaccctgt ggccgcggcg
     2581 gcagtattcg tagttccgct gctcgcgatc cggaccgcaa tgatgatggg caattgggcg
     2641 cagcacagct tcgtctgcgc cgaacacccc gaagacccct accgatcgag cattacctgc
     2701 atcaacactc gctacaatcg gcgctgcttc aacgacggat accacatcct ccatcacgtg
     2761 aagcctcgct gccattggag cgaacacccc gatgagttcc tgcgcgcgct gcccgagtac
     2821 gcgcagcacg acgcgatcgt gttcgacggc atcgactatt ttcagatctg ggtttacttg
     2881 atgttcggca agtacgaccg gctcgctcag catttcgtgc gtctgcccgg ggcgccggag
     2941 cgcagcccga gggaagtcga gcagttcctc aagcagcgtg tgcgacgcgg gcccgagcct
     3001 cgcccaaaca ttagcgcggc ctcggtcgcg taacgcgggt gagcctcggg cctggtccga
     3061 gtcgagtccc gatgtcgatc cgcaagcaaa ccctcagcgc tgcgggagta cgccaggcgc
     3121 tccgggccca tgccgacccg gagcgagccc gtgcgttggc tcggttcttc aagaccggac
     3181 ccggcgagta cgctgccgga gataccttct ggggcatcgg cgtgccaccg caacgcgcga
     3241 ttgcgcggca attcgccgct ctgccgctcg atgaactgtt gaaactgttg cagtccccgg
     3301 tgcacgagca gcggctgacg gcgctgttga tcttggtgcg tcaattcgag cgggccgacg
     3361 agcacgggcg ccaggagcga ttccgcttct acgtgcggca cttggcctac gtgaacaact
     3421 gggacttggt ggatagctcc gcgcacaaga tcgctggcga gtatctacta tcgcggacac
     3481 gcggtgagcg cgaattgctg taccggctcg ctcgctcgaa gaatctttgg cgccgacgcg
     3541 tctcgatcat cgcgagcgcc gcgttcatca acgcgggtga cgctcgcgat accctcgccc
     3601 tcgccgagat cctattgggt gatgagcacg atctgattca caaggccgtc ggttggatgc
     3661 tccgcgaagt cggcaagagg atcgatcgca aggtgctcct ggagttcttg cgccagcatg
     3721 cggtggcgat gccacgtaca gcgctgcgct actcgatcga gcacttgccg gcttccgagc
     3781 gcgcagcttg gttggcaatg cgcgccgaga gagctgcacg gccaaccatg cctcgcgtcc
     3841 gcacccgtgg ggcgagccca cgcgccaaac tccagcccga agctgaactg tccaaaccgc
     3901 ttcttcaaaa gaagtgagcc gcccggcgcc accgagggtt cctacgcgaa gtgacgccct
     3961 tcgcccgttc gcgacgcgcc caaagtcatt ggatctggcc ccgtctctgc gcgtgcgccg
     4021 cattggcggc cgcttgtagc gctgcgagcg tatccgatga ttcgtcttcc ggggccggtt
     4081 cgagctccgg cggtcacggc gtgagcgtct cgagtcagcc ctcgaatcga cccacggcgg
     4141 gcgcgggctc cccagcaaat gcgggggcga gctccagtcc gagctcggca gtagcgggct
     4201 catctgcggc gggcagcgcc gcagctaaca gcggtggacg cggcagcgtg gtgccaatcc
     4261 cggaagactg cagcgcggaa acgctcggag agccaagccc agtgcgaagg ctgagccgcc
     4321 tcgaatacgt caatagcctg cgtgctctcc tggccgaata cgggctcgaa cgagagctcg
     4381 atgcgagcag agagctgctc gcccaacttc cgcccgatgg cgaaaactct catctgttca
     4441 gcgaaatgga tgcgcgtacg agcgcgcgcc acgtcgacgc cttttacgcc gtggccgacg
     4501 acctcgcgac gcgcttgagc cgtgcaccca cactcactcg gatcgcgggt gagtgcgccg
     4561 cgcaaagccc ggtcgaacga gcctgcatcc agagcttcgt tgaccgcttc ggtcgactcg
     4621 ccgcgcggcg cacgctgtcc gcggaacaga gccgacgcgc catggagctc tatgacggga
     4681 gcttgaccgg agcggaaatc gtccgccgtc tcgtgttcac cttcttgcta tcgcccgagt
     4741 ttctctacca tctcgagctg gagggcctcg aatccagcac ttcaccgcgg gcactcgagc
     4801 tggatgggcc ggcgctagcc caaagactca gcttccactt cctgcgtcag ccgccgtcga
     4861 gcgcagtcct ggatgccgcc gaagccgggc aactttcgac gaacgcgggc tatgaggcgc
     4921 tggtggacga gctgttcgag cgttccgaga accgagcgct cgtctctagg ttcttccacg
     4981 agtggtttca actcgacggc ttcgccggct tcgcggacac gcgcgcgttc caggccttcg
     5041 cggcgggaac caccccgaac ggcgagttgt acgcggacat gttgaaggag gtcgacgcgt
     5101 tgattcatcg cttcaccttc gaatcagccg gaacctacgc ggacctgctc accactcggc
     5161 tttcgctgac ctcttcgagc gggctcgcgt cgttgtatgg ggtagcgccc tggagcggtg
     5221 ctggacagcc gccgagcttc gcggcggagc agcgggcggg gctgctgacg cgcgcggcgc
     5281 tgctcgtcga agggaacgaa atgaccaacc ccatcaagcg cggcgcattc atcttgaagc
     5341 acgtgctctg cgtgccgctg gagcctccct cgaacttgcc cgcagaagcg ctggcgttgc
     5401 cacccgcgaa cgcggcccag accacgcgca ctcgctttca aaacaagact gcgccgcgcg
     5461 agtgcgcggg ctgccacgaa ctcatcaatc cgctgggctt tgcgctggag gcttacgacg
     5521 cactcggccg cttccgaacc gaggagcagc tcttcgacga cgcgggacga gctcaggcca
     5581 cactacccgt tgacgcccat gtcacgttga gtttgggcgg cacgcgcgcg gaagtcagta
     5641 gtccagtcgc gttcagtgat gcgcttgcgc aaagcgccga ggccgctgcc tgttttgctc
     5701 gccagtactt ccgcttctcg gtgcggcggg acgagggtgc actcgacgcc tgcaccattg
     5761 ctcgagttca gcagagtttg cgcacggggg gcctcaaagc cgctttccgt agcctggttt
     5821 tcagcgaagc attcaaacat cgcgtcatcg aggctccatg aagatcgatc gcatcagccg
     5881 ccgcatgttt ctgcaaggtg tctccgggag cctattggcg ctaccattct tgccgtcgct
     5941 cgtcgccaga agcgcggaag cccaatcggc cgcggcgccg ctacgtctga tcaccatcaa
     6001 gagctacagc gcccaaaaca ttcgcgattt ctacccggct cgggctgttc caggctacac
     6061 acttcgcgga tacggcggtg acgagacggg cggagccaat ggcaaacaag acggcacgct
     6121 cgcgttgacc cagagcttga gcgcgaacag cgggaaacac tcgaacggaa gcgtatacac
     6181 aggcaagcaa gcaccgctga gagacttcgc gtcgcccgga atttctcgta ttctgggtcc
     6241 cgcgctgaat ccttatctcg acaagctctt gctgctgcga ggcttggact tcttaccgga
     6301 cacgaaccac aacgacggcg ggatcttggg caacttcggg ggcggcgcca tctcggatag
     6361 tgcgggttcg atccgcgagg tccccactat cgatcaggtc ttggcctact cgcccaaatt
     6421 ctactctacg gcgccgcccg gcggtcgaag ccttcacttg tcgcccggtc gccagaacac
     6481 gttctcctat acgcatgctg ggaagaaggg cggcccggtc acccaggtgc aagccgacac
     6541 cgatccgcgt ttggtgttcg accgcatttt tgcgaacgtc tcggcgccgg ccgccgaagg
     6601 cgtgaacccc aattcgttgc tcgtgaaccg cgtgatagag gattaccggc gcttgcgctc
     6661 gcacccgcgc atgagcgcag aggaccgcgc gcggatggag cagcacatcg cgttgatggc
     6721 agagctcgag gcccgtctgc aatccaagcc cggtgccgcg tgccaggcgc cgccgcgccc
     6781 cgccgcgctc ggcaacgtcg gcgtgaacgt gatgagcctg cggcaatctt acgctgccat
     6841 gctggacatc ttggcggcag ccattcgctg tgacctcacg cgcctcgtta ccctcgacgt
     6901 atacaaagcc attggggcca gcgcgggcga agagcagggc ttcgagcaca gctgcgcgag
     6961 ctgccaaggt aacccaaacc ctaccgactg gcatcgcgcc gcccatgatt gggatcaaaa
     7021 gccgcagcga gagaaggtgg tgagcatcaa cgaatggatc gccaaagaag tattcgcggc
     7081 cctgctgagt cgactcgacg tgcccgaggg caacgacaaa acctttctcg acaactcgct
     7141 cgtgctttgg ggcaacgaac tcggcatgaa tcacctcaac tacagcgtgc ccgtggtgat
     7201 ggccggcagc gcgggcggcc gcctcgcgac gggccgctac atcgactaca tcgactggaa
     7261 ccagcccgtt cgcttcagcc aacacgatgg catggtgatc gaaggcgtcc cttacaaccg
     7321 cctcttggtc accatcctcc aagcccttgg actcagccct gaggattacg aaaccgagcc
     7381 caatgcgggc tacggcgaga tcacccctct cggcaagccc gccactgctt tcgcgaccga
     7441 ctacgactac acccaaattg gccaacccct ccccaccctc ctgacctaac tcgcgccaac
     7501 ggtaacccgc ggtcccccac tcttacggct cgaaatacct gcctacctgc tcccgatact
     7561 cctccggcac atcccctcct tccaccgctc ctacctcccc gttccctcga ctcaacagac
     7621 tgcccatccc cacctcgtta gcggtctctc ccgctctccc cggcgcccgc ccaaaactcc
     7681 tccccgccaa agactccccc ggtaaccaac gcgcctccgc gcgcgcccga aactcctcac
     7741 tgcgcaccgc ctccggcttc gtgcgcccgt gatccccgcg tcccggcccc gatccgggtt
     7801 gccctgaagc tgggccgcca ttctcactgg atggcgcacc cgtgtttgct ttggatccgg
     7861 gatcgctccc gggtgaagcc atcccgggcc caggcatcgg caacggcagc ttcgataaac
     7921 cacgctcggc ttgcgcgcgc ccccgctcgg cctcaccgag cgcacgttcg cgctccgcgt
     7981 ccagattgtc ttctcgagcc aactcccgca gcgcgcgggc cagggcttca cgtccttcgg
     8041 gcgtctcgag ctccttcagc atcctgctca actcttcgcg agacagcgct tgggcccccg
     8101 gcgcggcgcc cgacagacgt tgcttgagag cctcggccaa acgcttgcgt tcctcgtcac
     8161 tcaaggattc gagcgccttg gcgagcgcct ccgccaaccg gcgctgtgcc gcagggttcc
     8221 cggtaccgtt cagctcggag agcgcctctt tgccctccgc ggagagtgcc ccctcgagct
     8281 gcttggcgag ctcgcggaga gccgcgctct cctgctcacg cttcgcgaaa ttcgaagact
     8341 gacgctccaa catatccgcg agcttctgcg ctttctgctc ccgggcgcgc tgtttggctc
     8401 gctccagggc ctcgcgagcc gcgtcccgag cccgcgcttc ggcacgggtc gcgaggcgtt
     8461 gcatctcctc atcgaacgcg atgatgtccc cgtcgccgag agccttctcg gccttgcgcg
     8521 tcgagggctc cgccccaagc tcggaaagag ctgcctccaa tcccgcgcgc tcagcgcgat
     8581 cagagaagcg ttgacgttgc ccgtctagct cttcacgtaa tcgagccaac tccgcctgcg
     8641 cctcacgctc ttccactcct tttgcgaggt cacgcctgag cttcttggct tgctccgcga
     8701 gttgtctcag ccgttgcgcg tccgcgttgg agaggctcgg tgcttgttcg agagcctcca
     8761 cgcgatccaa cccccgcacc gtatccaact tcacgcgagc gctgcccggt acgatgggag
     8821 cttgggcgcg ctgtgggggg tgcgtggcga gccacaccga ggccgcgaag acgggaactg
     8881 caaacgcctg cctcggcgtg aaaagacgca gactgagggt cccgcgcgga acggtctgca
     8941 acgctcgctc ggcttgggca cgcaacaacg cgcccaccgc ggatgggcgg ggctcggtct
     9001 cgagcgcggt cacgagcaat tctccggtcg cgaggcggtg atccaagaac aacgccactt
     9061 ggctcaggtt ccagcggcgc ttgaatccca agatggcccc cgcgactgca cctaccgcga
     9121 ccgcgcccgc ggcaacaccg accaacgccg cccagccgtg caccggaatt ctcaaccagg
     9181 tcgcagctgc gatcagcggc aaagcagccg ttccgcccca gcacgcgcca gcaatcgccg
     9241 tctgtaacgc ttgtcgaccc catatctggc gtgccatgcg ccacagagaa ttcatgaacc
     9301 cggacgctgg cttcgggctt aactcggttg cgctgctgct catcggatcc tgctcaccgc
     9361 gtcatgctct tcgggtcatg ctcatggctc gacgacattc gtttcggcgc ggcgctcgcg
     9421 tcgcggcatt ccgtccgccg cccaggactc aggttggcag actcgagcca cttctctgcg
     9481 gccgaacacg agcgtagcgc cagccactca ggctcgccag cccgccccac gcgccgtcct
     9541 cgcccggggc gaaagtctcg agtaggctcg cacacgagcg ggcagcacga accgcgatac
     9601 gtgtgcctgg attcgcatgc ataaaaacaa aatcacggtc cccgatttga tcgcacgcaa
     9661 agcagcaggt ccgaagatca gcgtgctcac cgcctacgat gcgaccatgg cgcgactgtt
     9721 cgacgacgcg ggcgtcgatg ttctgctcgt cggagactct ctgggcatgg tcgtgcaagg
     9781 gcaagccaac acgctcggcg tcacgatcga agacatctgc tatcacggac gggccgtcgc
     9841 tcggggtacg cggcgcgccc acctggttgg ggatttgccc ttcatgagtt tccaaggctc
     9901 ggcagacaag gcgctcgaat ccgccgggcg gctgttgaaa gaagggtcat tcgaagccgt
     9961 caagctcgag ggtggagccc gttacgccga gcacgtgcgc ttgctcgtcg aatccgggat
    10021 cccgaccatg gggcacctgg gtctgttgcc ccagtcggtg cacgcgatgg gtggctttcg
    10081 agtgcaaggg cgcgatgacg cttcggccga gcgcattttg cgtgatgcgc gtgtactcga
    10141 ggaggcgggc gcgtatgcga tcgtgctcga aggtatccca atggatctag ctcgccgcgt
    10201 gacggagagc ataaccattc ctaccatcgg catcggagcg ggccctcact gcgacgggca
    10261 ggtgctggtt tgctatgact tcttgggcat gtactcggac attcgaccca agttcgtgaa
    10321 gcgcttcgcg gagctgggtg attgcgtggc ggccgccgcc caggcctacg tgtccgaggt
    10381 gcaaagcggc gcgttccccg gtatcgagca ctcatttggt gccgcaccgc ctcgaccccg
    10441 tccaaagacg gaggagacat atccgcggct ggatccggca gcacccgcga catacggacc
    10501 ggccagcgag gaatcggtat gaagccgcag ctcatcgagc gcgcgcagga cttccgacgt
    10561 cgctgcgata ccgtgcgtgc gagttcgcga gcggtgggcc tcgttttgac catgggtgcc
    10621 ttgcaccgcg gccacatggc gctcgtgcaa gccgccaagg cgcgcgggct ggtgcccctc
    10681 gtcacgatct tcgtgaatcc cactcagttc ggtcccaacg aggactacgc gcgctatccg
    10741 cgcacgctcg aacgcgacct cgagttgtgc ggtgaacacg gtgtggagag cgtcttctgc
    10801 ccgagcgtcg aagaaatgta cccgcccggt gatcggactc gtgtcagggt ctcgggtctc
    10861 acgcaagcct tgtgcggccc caagcggccg gggcacttcg aaggcgttgc caccgtcgtg
    10921 accaagttct tcgcgttggc ggggccgtgc gtcgcattct tcggccgcaa agattatcag
    10981 cagctgcaag tgattcgccg gttcgcgcgg gacttgctgc taccggtcga ggtgaagggg
    11041 gagcccaccg tgcgcgagcc cgacggcttg gccttgagct ctcggaacag ctacctgagc
    11101 gccgacgaga gggcgtgtgc actggtgatc ccgcgcgcac tctcggcggc agttgcccgg
    11161 catcgggccg gggaacgccg cgctgaaccg ctgcgcacgt cagtccaagc ggcactcgag
    11221 gccgcgggca tgcgcgtcga ctacgtggag ctggccacgc cagaagaact cgaaccgatt
    11281 tctgatgcga gcgtcgtggg tgagcgggct ttgctcgcac tggccgcgtt cgtgggcaaa
    11341 acccggttga ttgacaacgt cgtactc
//
LOCUS       DASTKU010000716         8607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_806091, whole genome shotgun sequence.
ACCESSION   DASTKU010000716 DASTKU010000000
VERSION     DASTKU010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8607
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806091"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VFQ61_25060"
     CDS             <1..545
                     /locus_tag="VFQ61_25060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957802.1"
                     /translation="TTVYAKEAVGGDGPHKDVMAFRVETRKGDNGNDKTESWQLPLAE
                     NPQRVVRYRELAYSPSTGMPENEDYWDPYKIHIDGSPDKISKGQWPEEYTEFKKPTGG
                     AVSSGGRIDLWKVINADESVNVNGKTFEHSIHFMKTGNSDPDSVKEYWYVRGIGKVRE
                     AGSSQTEELISYTVGGKKGP"
     gene            542..1417
                     /locus_tag="VFQ61_25065"
     CDS             542..1417
                     /locus_tag="VFQ61_25065"
                     /inference="COORDINATES: protein motif:HMM:NF020916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HET9957803.1"
                     /translation="MSQSALVALNGSREGDEQMTAEREETKYLFPAEHVDALVFELNR
                     RLPAHRFTGEGANHLPDPHHFVTTIYFDTPSRRQFRSAIADVEHNVKIRAKEYYDLHP
                     SLAELATDPTEIVRYQPWVWFEVKRREGSRTLKQRFRLPKRDVPRIFSEGCISPGLSA
                     DMILSDAELEHSAAGVLELVKCVEQVGEPLRADCLVNYRRLSWQDAQGFLRVTLDLGL
                     SYYSPPPELWELQHALVRAVLGPHRGSEPHAVLEIKRRAALPSWLTELLSRADVRPVP
                     FSKFVASAGAVHGYS"
     gene            1404..3533
                     /locus_tag="VFQ61_25070"
     CDS             1404..3533
                     /locus_tag="VFQ61_25070"
                     /inference="COORDINATES: protein motif:HMM:NF014719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957804.1"
                     /translation="MATADRTSTRLRTVPIKVSQRIKTRRRISVLAAWVLTALIAGFY
                     LLLPNGDERVSLPTARESVEEQGEPRRLDIVGVAPSDVSAGSAVTVAFTGAEHDERVA
                     AFVGKTPMEVLTRRQGSLVVRVPAELTPGRVKLRVVEGTERSKPYDLQVKSPNWQKPF
                     RNLVGGFALLLFGISVLAKGAREAAGLRSAQTLARLARRGPSALGFGALIGALVQSTA
                     GAASVLAGLVASSLVAVAPAALAFLGAQIGAATAPLVTGLVDPREGLVLIAIGALWHG
                     LASDRRSNAMGRFVLGAGLIAFGLQTLRPGFEPFVQNPRLLSLISELRADSIGHIAAA
                     ASLGALLVALLQGPAPVLILVLVLAETTAQWDLTTALAVLSGSGLGAAMGALVTTPAG
                     RKCRQLATLNLSLGLLATLIAASTVGIWSRVADLVVPGVPHEIQWGRRVLLPNLGVHL
                     AVAFALSQLSSALLLWPLVRPLASWLDRRLPSSSLHNLARIGDPVGVLRNQLGAVVKA
                     YAQALPHLRQLVLEGSRQAGRDAEHVLTDARAALEEVLAGPIFSLPDTLDGRSLSRTA
                     FTTLQLGRALEALSRQAERLVDVRVSASSSGEPSPLPDADSATLTQMHELLSEGIASV
                     FDALSEAKAIDIEHARAREIRMNGLESRARGSVLADQREPGEMRCYLAVLELADAYEN
                     VGNQLYRSSESLAENFVRTSAAPARVM"
     gene            complement(3562..4287)
                     /gene="recO"
                     /locus_tag="VFQ61_25075"
     CDS             complement(3562..4287)
                     /gene="recO"
                     /locus_tag="VFQ61_25075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET9957805.1"
                     /translation="MNRAETVLDSRGLLLRRVEFGESDLVLSFFTERLGRISALARGA
                     RRSRKRFGGSLEPFFTLALRLQPPGRGELFTLVEASIVQARTRLLSDLNGMNAAGRAL
                     SWLRRAAPAHTPEPALWEMIEALLEKLADPQSSGGAEGELAVAGMRLLSALGFAPELG
                     RCVRCGTPCPPERSGSLDVPSGGLVCRACGGGRKRLSASVRQELMAAMRGEMLTVPLT
                     HEALTQALDLIEGVLAHHAGVEG"
     gene            complement(4284..4730)
                     /locus_tag="VFQ61_25080"
     CDS             complement(4284..4730)
                     /locus_tag="VFQ61_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9957806.1"
                     /translation="MRGVGQYLREQRESRGMSVEEISRATRVPVSSVERIEADRYDEL
                     PGEVFVRGFLKSYARALGVPADEVMARYTASRRVAWVTPLPISSVNPRPARGRRFGVA
                     IAFVLLLILFTLALSIVLKPRGEDMPQELSFASFAHGTLTRFERGA"
     gene            complement(4742..5509)
                     /locus_tag="VFQ61_25085"
     CDS             complement(4742..5509)
                     /locus_tag="VFQ61_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9957807.1"
                     /translation="MWAAVSAGCGGSRTQGAQDPTRQAETEYDLARDLWLQRGQPREA
                     LNHALKAIELDEQNAEAQHLVALLYLDFCARSALECRLPQAEKHARAALDQKPDLREA
                     RNTLGVILIHAHRPRDAIQVLKPLSDDILYQTPEKAWGNLGWAYLESGQLELAEDALR
                     RSIAAQPLFCVGSYRLGLVYEKRMHYPAALDALTRALETEAPECSRLQDAFSARARVL
                     AHLGRTPESLSDLERCVELSQSTETGKNCRSMLQKLK"
     gene            complement(5588..6259)
                     /gene="tsaB"
                     /locus_tag="VFQ61_25090"
     CDS             complement(5588..6259)
                     /gene="tsaB"
                     /locus_tag="VFQ61_25090"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET9957808.1"
                     /translation="MRILGIETSSRRGSVALLEDERCVAQIEHEQPNAHAERILTLLN
                     RAFSEAGWSRTSLDRIAVGTGPGSFVGLRVGIAVAEGLGIGLSRPVVGVSSLQAMLRA
                     TPPDLCEARIALLDARRGEAFAAAEDNLGRALLPPCTIPLERLATELLAIAPRPLLIG
                     EILVPDFDATHLRGPHTDLPHARWVAELARRLDPSVAPAVPEYVRGPGATLPKLPPSP
                     LRAQS"
     gene            6540..7781
                     /locus_tag="VFQ61_25095"
     CDS             6540..7781
                     /locus_tag="VFQ61_25095"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET9957809.1"
                     /translation="MVVVYYWSVPHVLPRFDYRVDYATAFDQGFQRESYEDAALIAPE
                     LGVFAIADGMGGHAAGEVAAALAIQEVKLALASRAAQRSVEQYLNQPDLTARRAVFAE
                     LKGAVERANQRVFAESCERSEHRGMGSTLDVVWLARGHAFVAHAGDGRVYVARSKTML
                     QVTQDHTVLAALGSESGRHSRYTALGAALTNAVGLREAVQVDSAFVDVSRGDRLLLCT
                     DGVYNQIHGEAELAELLRHGDAAHSAQALVERARQGGRDNATVVLVCIGERFVRRGER
                     DRGLNAGDLERARQSPLLVDLPESYVLSALTAAVEVEVSAGERIPRLVASDLVAYIVL
                     DGVVFHPASGRRVGAGALVHPESLAGISGSVEPPVAEETTRLLRLRADDFAEVCSEPR
                     LAAELHRRLSQHLARLLVRGP"
     gene            7966..8043
                     /locus_tag="VFQ61_25100"
     tRNA            7966..8043
                     /locus_tag="VFQ61_25100"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8001..8003,aa:Pro,seq:ggg)
     gene            complement(8408..>8607)
                     /locus_tag="VFQ61_25105"
     CDS             complement(8408..>8607)
                     /locus_tag="VFQ61_25105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957810.1"
                     /translation="SLETCRARSGDAACDVTFVDERPAGERARVGLAECNARSSHTQF
                     RFEAQRRTLLLSPLRRLRACV"
BASE COUNT         1484 a         2672 c         2894 g         1557 t
ORIGIN      
        1 tcactacggt gtacgcgaaa gaagcggtcg gtggtgatgg gccgcacaag gacgtgatgg
       61 cgttccgggt cgaaacccgc aagggtgaca atggcaacga caagaccgag agttggcagc
      121 tgccgctggc cgagaacccg cagcgggtcg tgcgctatcg ggagcttgcc tactcgccgt
      181 ccacgggtat gccggagaac gaagattact gggatcctta caagatccac atcgacggct
      241 cgcccgacaa gatcagcaaa gggcagtggc ctgaagagta taccgagttc aagaagccga
      301 ccgggggcgc agtcagctcg ggtgggcgca tcgatctatg gaaggtcatc aacgccgacg
      361 agtcggtgaa cgtgaacgga aagaccttcg agcactccat tcacttcatg aagaccggca
      421 actccgaccc ggactctgtg aaggagtact ggtacgtgcg cggcatcggg aaagtcaggg
      481 aagccggcag cagccagacc gaagagctca tctcttacac ggtgggaggc aagaaagggc
      541 cgtgagtcaa tccgcgctgg ttgcgttgaa cggctcgcgc gaaggcgacg agcagatgac
      601 cgccgagcgc gaggagacga agtacctttt tccggccgag cacgtagacg ccctggtttt
      661 cgagctcaac cggcgtctac ccgctcaccg tttcaccggc gagggcgcga atcatctgcc
      721 cgacccgcat cacttcgtga cgacgatcta cttcgatacc ccatcgcggc ggcagttccg
      781 gtctgcgatc gcggacgtcg aacacaacgt gaagatccgc gcgaaggaat actacgatct
      841 gcatccctcg ttggcggagc tcgcgaccga tcccacggaa atcgtacgct atcagccgtg
      901 ggtttggttc gaggtcaagc ggcgcgaagg ctcgcgcacg ctcaaacagc gctttcgatt
      961 gccgaagcgc gacgtgccac ggattttttc cgaaggttgc atatcgccgg ggctctcggc
     1021 ggacatgatc ctatcggacg cggagctcga gcactccgcc gcgggggtgc tcgagctcgt
     1081 gaagtgcgtt gagcaagtgg gggagccgct gcgcgcggat tgcttggtca attaccggcg
     1141 gctctcttgg caagatgcgc agggcttcct gcgcgttacc ctcgatttgg ggctcagcta
     1201 ctactctcca ccgcccgagc tgtgggagct ccagcacgct ctcgtacgcg cggtgctcgg
     1261 gccccaccgc ggttccgagc cccacgccgt gctcgagatc aagaggcgcg cggcgttgcc
     1321 gagctggctc accgagctgt tgtcccgggc cgatgtgcgt ccggtcccat tcagcaagtt
     1381 cgtggcttct gcaggagcag tgcatggcta cagctgatcg aacgtcgacc cggctacgca
     1441 ccgtaccgat caaggtctcg caaaggatca agacacggcg gcggatttcg gtcctggccg
     1501 cgtgggttct gacggctctg atcgcgggct tctatctgct gctgcccaac ggagacgagc
     1561 gggtttcgct cccgacggct cgcgaatcgg tggaagagca aggcgagccg cgccgtctcg
     1621 acatcgtggg cgttgcgccg tcggacgtga gcgcgggcag cgccgtcacc gtggcgttta
     1681 cgggcgctga gcacgacgag agagtcgcgg cgttcgttgg caaaactccg atggaggtgc
     1741 tgactcggcg gcagggttcc ctggtggtgc gcgtgccagc ggagctgact cccgggcgcg
     1801 tcaagctgcg cgtcgtcgag ggtactgaac gcagcaagcc ttacgatctg caggtcaaga
     1861 gcccgaactg gcagaagccg ttccgcaatc tcgtcggcgg cttcgcgttg ctgttgttcg
     1921 gcatctcggt gttggccaag ggggcgcgtg aagccgcggg cctgcgcagc gcacagacgc
     1981 tggcgcgctt ggcgcggcgg gggccctcgg cgctgggttt cggcgcgctc atcggcgcgc
     2041 tcgtgcagtc cacggcgggc gcggcttccg tattggccgg cttggtagcg tcgagcttgg
     2101 ttgcggtcgc tccggcggcg ctggcttttc tgggcgcgca gataggagca gccacggctc
     2161 cgctggtcac cggcttggtg gacccgcgtg aaggcctggt gctcatcgcg atcggcgcgc
     2221 tctggcatgg gctggcgagc gatcggcgat cgaacgccat gggccgcttc gtgctcggcg
     2281 cgggcttgat cgcatttggg ctgcagacgt tgcgcccggg attcgagcct ttcgttcaga
     2341 atccaagatt gctttctctg atttccgagc tccgcgcgga tagcatcggc catatcgcag
     2401 cggccgcttc gctcggtgcg ctcttggtcg ccctgctgca aggccccgcg ccagttttga
     2461 ttctggtgct cgtgttggca gagacgacgg cgcagtggga cctcaccacc gctctggccg
     2521 tcctctcggg ttcgggcttg ggtgcagcca tgggcgcgct cgtgacgacg cctgcgggcc
     2581 gcaaatgccg tcaattggcg accctgaatc tgagcttggg tctgctggcg accctgattg
     2641 ctgcgagcac ggtagggatt tggagtcgag tcgcagatct ggtcgtgccc ggtgttccgc
     2701 acgaaatcca gtggggacgc cgcgtgctgc tgcccaactt gggcgttcac ctggcggtag
     2761 ccttcgcgct gtctcagctg agctcggcct tattgttgtg gccgctggtg cgtccgctcg
     2821 cgtcctggct ggatcgtcgg ctgccgagct cgtcgttgca caacttggcg cgaatcgggg
     2881 atccagtggg tgtgctgcgc aatcaactcg gggcggtggt gaaggcctac gcccaagccc
     2941 tgcctcattt gcgccagctg gtgttggagg gcagtcgtca agcggggcga gacgccgagc
     3001 acgtcctgac cgacgcgcgt gctgcgctcg aggaagtgct ggcgggtcca atcttctcac
     3061 tgcccgacac gttggacggg cgctctttga gtcggaccgc attcacgacc ctgcagctcg
     3121 ggcgggcact cgaggctttg agccgtcaag ccgagcgcct ggtggacgtg cgcgtctcgg
     3181 cctcctcgag cggagagcct tcgccgttgc ctgacgcaga ttccgcgacg ctcacccaaa
     3241 tgcacgagct gctcagtgag ggcatcgcgt cggtgttcga cgcactcagc gaagccaaag
     3301 ccatcgacat cgagcacgcc cgcgcgcgag agatccgcat gaatggcctg gagtcgaggg
     3361 cgcgaggctc ggttctggcg gaccagcgcg agccgggtga aatgcgctgc tacctcgcag
     3421 tgttggagct ggcggacgcc tacgagaacg tcggcaatca gctgtaccgt tcgagcgaga
     3481 gcctggcgga gaatttcgtg cgaaccagcg ccgcgcccgc gcgcgtgatg tgactcggag
     3541 ctcgaataag gctcgcggcc ttcaaccttc tacgcccgcg tggtgtgcaa gtacaccttc
     3601 gatgagatcg agcgcttgcg tgagcgcttc gtgagtcaga ggcacggtca gcatttcacc
     3661 ccgcatcgcg gccatgagtt cttggcggac gctggcgctc agtcgcttgc gtccgcctcc
     3721 gcaagctctg caaaccaaac ccccgctcgg aacgtcgagg gagccggacc gctcgggagg
     3781 gcagggggta ccgcatcgaa cgcagcgccc gagctccggc gcgaatccga gtgcggagag
     3841 taaccgcatc cccgcgaccg cgagctcccc ctctgctccg ccgctcgatt gcggatcggc
     3901 aagcttctcg agcaaggcct caatcatctc ccacaacgcc ggctccgggg tgtgggcggg
     3961 ggcggctcgg cgtagccaag acagcgcgcg gcccgccgca ttcatgccgt tcagatccga
     4021 aagcagccgg gttcgcgctt gcacgatgct ggcttccacg agcgtgaaca gttcaccgcg
     4081 accggggggc tgcagccgca aggcgagggt gaagaatggc tcgagcgagc ccccgaaacg
     4141 cttacggctg cgccgggcgc cgcgggccag cgccgagatc cttccgagcc gctcggtgaa
     4201 gaacgacaag accaagtccg actcgccgaa ctccaccctt cgtaacagga ggcctcggct
     4261 gtcgagcacc gtttcggcgc gattcacgct ccccgttcga acctggtcag cgtgccgtgc
     4321 gcgaagctcg cgaacgataa ctcttgaggc atgtcttcac cgcgcggctt caacacgatg
     4381 ctcagcgcga gcgtgaacaa aatcaggagc agcacgaacg cgatggcgac cccgaagcgg
     4441 cggccgcgcg ctggcctcgg gttgacgctc gaaatcggca acggggtcac ccatgccacg
     4501 cgccggctcg cggtgtaacg cgccatcacc tcgtccgcag gcacaccgag agcacgcgcg
     4561 tacgacttca aaaagcctcg gacgaacact tcgcccggca gctcatcgta acgatcagct
     4621 tcgatccgtt cgacactcga gaccggaacg cgggtggcgc gcgagatctc ttcgacgctc
     4681 atccctcggg actcgcgttg ctctcgcagg tattggccga caccacgcat ggggcccgag
     4741 actacttcag cttctgcaac atggagcggc aattcttgcc cgtttcggtg ctttgcgaca
     4801 gttcgacgca acgttcgagg tctgacaggg attctggagt tcgcccgaga tgtgcgagca
     4861 cacgcgcacg tgcagagaac gcgtcctgca gtcgactaca ctccggggct tccgtttcca
     4921 gtgccctcgt aagcgcgtcc aaggctgccg gatagtgcat gcgtttttcg tacaccaggc
     4981 cgaggcggta gctgccaacg cagaacaacg gttgcgccgc gatgcttctg cgaagagcat
     5041 cttccgcgag ctcgagctga cctgattcta agtaagccca gcccaagtta ccccaagctt
     5101 tctccggtgt ctgatagagg atgtcatcgc tcagtggctt caagacttgg attgcgtctc
     5161 gaggccgatg agcgtgaatc agaatcactc ccaaggtgtt gcgggcttca cgcaaatcgg
     5221 gcttttgatc gagcgccgcg cgggcgtgct tctcggcctg aggcagccgg cattcgagcg
     5281 cgcttcgcgc gcagaagtcc aggtaaagga gagcaacgag gtgctgggct tcggcgtttt
     5341 gctcgtccag ctcgatcgcc ttcagcgcgt gattcaatgc ctcgcgcggc tggccgcgct
     5401 gcagccaaag atctctcgca aggtcgtatt cagtttctgc ttggcgcgtg gggtcctgtg
     5461 ccccctgagt gcggctgccg ccacaacctg ccgaaactgc agcccacacc gcgagcgcga
     5521 ctgccgttga gcgaggtccc gtgacgggcc agcggcggag tgagcggttg caggggacca
     5581 taccgagtca ggattgggcg cggagcgggg aaggcggcag tttgggaagc gttgctccgg
     5641 gaccgcgcac gtactcgggt acagcgggag cgactgacgg atcgagtcgg cgggcgagtt
     5701 ccgccaccca acgcgcgtgc ggcaagtccg tatggggccc gcgaagatgg gtggcatcga
     5761 agtcggggac caagatttca ccgatcagga gcgggcgcgg agcgatggcg aggagttcgg
     5821 tggctagtcg ctccaagggg attgtacacg gcgggagtag ggcacggccc aagttgtcct
     5881 cggcggccgc aaacgcttca cctcttcgtg cgtcgaggag ggctatccga gcttcgcaaa
     5941 gatccggggg tgtggctcgc agcatggcct gcagcgatga aacgccgacc acgggcctcg
     6001 acaagccgat gccaagacct tcagccactg caatgcccac gcgcaggccc acgaatgatc
     6061 ctggccccgt gcccacggca atgcgatcca ggctcgttcg cgaccatccc gcttccgaaa
     6121 aggcccgatt gagcaaggtc agaatgcgct cggcgtgagc attgggctgc tcgtgctcga
     6181 tctgggcgac gcaccgctcg tcttccaaca gggcgacgga cccgcgccgg ctggaagtct
     6241 cgattccgag gatgcgcatg gccgcctctt agttctgggc gttgcagtgt gcaaccaact
     6301 cgttgaggtc tccgtgtgaa tttggatgag gggttgtgtc gccccgaatc taacctgcga
     6361 gtcacgccga tgagtcgcct gcgagcggac ccctgattcg gagaagtggg gcgaaaccga
     6421 cggcatgtgc gccgagttcg caccgcacgg cctgtgcgca gatcggcgtc cgcggcaatg
     6481 tgagtttcct ctccaatgag cgattcgatc acacgacagg ggatttgggc gatcgggccg
     6541 tggttgtcgt ctactattgg agcgtgcccc acgtcttgcc ccgcttcgac tatcgcgtcg
     6601 actacgcaac cgcgttcgat cagggctttc agcgcgaaag ctacgaagac gcggccttga
     6661 ttgcccccga gctcggcgtc ttcgcgatcg ccgacgggat gggcggccat gcagccggcg
     6721 aagtggcggc tgcattggcc attcaagagg tgaagctcgc gctggcgagc cgagccgctc
     6781 agcgctcggt cgagcaatac ctgaaccaac cggatttaac ggccagacgc gccgtattcg
     6841 ccgaactcaa aggcgcggtc gagcgggcca atcagcgcgt ttttgcggaa tcgtgcgaac
     6901 gcagcgaaca tcgtggcatg ggctctacgc tcgacgtcgt gtggctggcg cgcggccatg
     6961 ccttcgtcgc gcacgcgggg gatggccgtg tctacgtggc tcgctccaag acgatgctgc
     7021 aggtaaccca agaccacacg gtgttggcag cgctgggctc ggagagcggt cggcacagcc
     7081 gatacaccgc cctcggggcc gccctgacga acgccgtcgg gctgcgcgag gcggtacagg
     7141 tcgattcggc tttcgtcgac gtatcacgcg gtgatcgcct gctgctttgc accgacggtg
     7201 tgtacaatca gatccacggc gaagccgagc tcgcggagct tctgcgtcac ggcgacgccg
     7261 cccactccgc ccaagccctg gtcgagcgcg cgcgtcaggg tggacgggac aatgctacgg
     7321 tggtgctcgt ctgtattggc gagcggttcg ttcgtcgcgg agagcgagac cggggcttga
     7381 atgcagggga cttggagcga gcgcgtcaga gcccgctgct cgtcgacctg ccggaatcct
     7441 acgttttgag cgcgctcacc gccgcggtcg aggtcgaggt gtctgcgggc gaacgcatcc
     7501 cgcgcttggt ggcgagcgat ctggtcgcgt acatcgtgct cgacggagtt gtgttccacc
     7561 cggcgagcgg gcgtcgcgtc ggtgccggcg cgttagtcca ccctgaatct ctggctggca
     7621 tcagtggctc ggtcgagcca ccggtcgcgg aagagacaac acgcctgttg cgattgcgag
     7681 cggatgactt cgcagaagtg tgcagcgaac cacgcctcgc ggccgagttg catcggcgct
     7741 tgtcacagca tcttgcacgc ctgctggtgc gcgggccgtg agcccaccgc gcgaagcact
     7801 tcgcgctcgc ggcaaagtag gctgccgagc caggatgtca gaaagcaccc gacaggcact
     7861 aattggggtt gcaatcttcg gcagaccgac tacgttcgcc gggccttgaa cacctgggcc
     7921 gcttcgcaaa aacgcagcga tttagggcgt gaaaggatgg tcgaacgggg cgtagcgcag
     7981 cctggttagc gcaccagact gggggtctgg gggtcggcgg ttcgaatccg cccgccccga
     8041 ccattgatcc tgaagtagtc cctcgtattg cacgggggct ctcgagtcca aacgggctcg
     8101 taaatccaga cttagctcca cctgtctggg tgcgagcctg tattggggac gagccgcgtc
     8161 cggggctgca cctcaaaggg agcagcttcc ggacgagcag catccaacgg acgcagcgcc
     8221 gcagtcgctc gggctgcgtt tgtcgctcgg gctgcgtttg gagtggcgct tcggcgactg
     8281 cgagccacca tgaccaccgg cggccaccgt cagtagtcac gttcgccgag gtgtaaggtt
     8341 ggggcgcttt gcgagtgcga ctacccgcgt ggtttacgac ctcgaaccgc cgaggcccgg
     8401 ggtgaactta aacgcaggcg cgcaggcgcc gtagtgggct cagtaggagc gtcctgcgct
     8461 gagcctcgaa gcggaattgg gtgtgggacg accgcgcgtt gcactcggcc aagccaaccc
     8521 tcgctcgttc acccgctggt cgttcgtcga cgaaggtgac gtcgcacgct gcgtctcccg
     8581 acctcgcacg gcaggtctcg agtgaac
//
LOCUS       DASTKU010000717         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_138196, whole genome shotgun sequence.
ACCESSION   DASTKU010000717 DASTKU010000000
VERSION     DASTKU010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138196"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFQ61_25110"
     CDS             complement(<1..241)
                     /locus_tag="VFQ61_25110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957811.1"
                     /translation="MTIRAFVPRVFRALVLLGGLALLVAGGLSIRVWVAGRAALDRAE
                     AAFDRGELEEAIRQAQRAATLQLPGAAHVDQAYLRL"
     gene            complement(238..1170)
                     /locus_tag="VFQ61_25115"
     CDS             complement(238..1170)
                     /locus_tag="VFQ61_25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET9957812.1"
                     /translation="MIARLWALSYNTYREAVRARILHGLFALALGAGVYAQVVAAFSN
                     RAKLRALSDLGAASVSIFAVFVAIVLGATSLYRELELKTIFPILSRPLERGEYLVGKF
                     LGALLTLSVFIAANTGALLMALGQAAGRSFLGSFGGALGVLVAAAIVAWRMPRLRTAL
                     PIPVALILLGLGYLLAGGAPDDRRVLVFSAWLTLCEVGIVMGVTSVFSSFSSPFLTAV
                     FSAGVFVVGRSAATLGELPVRQFGEQVRAIGQGLSRVLPNLMLYVPERRLLTGEAAML
                     PIERYLAYATLHAAAWAVGLLALAAVLFRRRDFL"
     gene            complement(1174..1899)
                     /locus_tag="VFQ61_25120"
     CDS             complement(1174..1899)
                     /locus_tag="VFQ61_25120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957813.1"
                     /translation="MRRASRFLGLAVLLCVCSSGLLAEAEPSRSSGSSLSRPERAELA
                     AGQRVERALEVHAGSGEYVGGLAYQVVRAAAPAVMLELTDLSNLQSMLPNTLGSRVVA
                     RDRDIVRVEVEQGVRPFVARYTIVMQPATAGEVRFWLDKTAPHDIEDVWGFFRVTPFG
                     KHSLLTVATAIDLGSGLVSALFKERVRALALRSATSIRDFVEPRCSGIQHLASLDQPG
                     FGAVEERPLQGLSPAAGRGVRRR"
     gene            complement(2166..>2615)
                     /locus_tag="VFQ61_25125"
     CDS             complement(2166..>2615)
                     /locus_tag="VFQ61_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957814.1"
                     /translation="VSVSAFVRPAGPTTLVSFSDTCEEAWRIPELGGRFEGNTNNASA
                     DYSAGCDYGGGSARGAPEQMLRLTLNRKRRFVFDLAGSDYRTLLAIRRGDSCPGEELP
                     NACAPGRAANRSFLDLTLEPGDYFVQVDGFGEASGHWVLDVYSATPE"
BASE COUNT          554 a          902 c          795 g          364 t
ORIGIN      
        1 ccaagcgcag gtaagcttgg tccacgtgcg cggcgccggg cagctgcagg gtcgccgccc
       61 gctgagcctg gcgtatggcc tcttcgagct ctccgcgatc gaaggcagcc tccgcgcgat
      121 ccaacgccgc gcgcccggcg acccacactc gaatcgagag cccgcccgcc acgagcagcg
      181 ccaatccacc caaaagcacc agcgctctga acacgcgcgg aacgaaggct cgaatcgtca
      241 taaaaagtcg cggcgccgga acagcaccgc cgcaagcgcc agtaggccta cagcccaggc
      301 cgcggcgtgc aaggtcgcat acgccaagta gcgctcgatc ggcaacatcg ccgcctcacc
      361 cgtgagcagc cggcgctccg gtacgtagag catcaagttc ggcagcacgc ggctcaagcc
      421 ttggccgatc gcccgcacct gctcaccaaa ctggcgcacg ggcaactcgc ccaaggtcgc
      481 cgccgagcgc cccaccacga aaaccccggc gctaaacacc gcggtgagaa acggcgacga
      541 aaacgaggaa aaaacgctgg tgacccccat cacgatcccg acctcgcaca acgtgagcca
      601 ggcggaaaaa acgagcacgc ggcgatcatc cggcgctccg ccggccaaga ggtacccgag
      661 cccgaggagg atcagtgcga ctgggatggg cagagccgtt cgcagcctcg gcattcgcca
      721 agccacgatc gcggccgcga ccagcacccc cagggccccg ccgaagctac ctagaaagga
      781 tcgcccggcg gcttgcccga gtgccatcag caatgcgcct gtgttcgcgg caatgaacac
      841 gctcaacgtg aggagagcgc ccaaaaactt gccgaccaag tactcacctc gctcgagcgg
      901 gcgagacaag atcgggaaga tggtcttcag ctccagctca cgatagagcg atgtcgcacc
      961 cagcacgatc gcgacgaaca ccgcgaagat agagaccgaa gccgccccca agtcgctcaa
     1021 ggcgcgcaac ttcgcgcggt tcgagaaggc ggccacgact tgggcataga ccccggcccc
     1081 gagcgccagc gcgaacaatc cgtggaggat gcgcgctcgc acggcctccc ggtacgtatt
     1141 gtacgagagc gcccagagcc gcgcgatcac gactcacctt cggcgaaccc cgcggccagc
     1201 cgccggcgag agcccctgaa ggggccgctc ctccacggcg ccgaacccgg gctggtccaa
     1261 gcttgcgagg tgctggatcc cggaacaccg gggttcgacg aagtctcgga tactggtggc
     1321 agagcgcaaa gcgagcgcac gcacgcgctc cttgaacaga gcgctgacca gaccagagcc
     1381 caggtcaatg gcagtcgcga cggtgagtag gctgtgcttt ccaaacggag tgactcgaaa
     1441 gaagccccaa acgtcttcta tgtcgtgtgg agcagtcttg tcgagccaga atcgcacctc
     1501 tccggccgtg gcgggctgca tcacgatcgt atagcgagcg acgaacggcc gaactccttg
     1561 ctccacttcg acccggacga tgtcgcgatc gcgagcgacc actcgcgagc caagcgtgtt
     1621 cggcagcatg ctctgcaggt tcgagaggtc cgtgagttcg agcatcacgg ctggtgccgc
     1681 tgctcgcacg acctgatagg ccaggccgcc cacgtactcg ccgctccccg cgtgaacctc
     1741 gagagcccgc tcgacgcgct ggcccgcggc gagctcggcg cgctctggtc tcgacaagct
     1801 cgagccactc gagcgtgacg gctcggcctc cgccagcaat cccgacgaac acacgcagag
     1861 caggacggct aagccgagga atctcgaagc tcgtcgcatg gccgctcaga ctaaccgctc
     1921 tcgagcgttc gagccagtaa tcggtgagtc gcaatccatg ctcgaatgag acactctgaa
     1981 gtgtcacggc tcagaacttg ccagcttcag cctcagccac acttcatcgc gccagaccga
     2041 ggacgagggg cgaagttcgc cagtggcagt ctcgtattgc ctcattattc cgcgctgaat
     2101 ggctcaagat gcacgccccg gcgaagcaac tcgcgcgttc tcacgctgca tccggttggg
     2161 cggattcatt caggcgttgc ggaatagacg tcgaggaccc agtgccccga agcctcgcca
     2221 aagccatcga cttgcacgaa gtagtcgccg ggttccagcg tgagatcgag gaaactgcgg
     2281 ttagccgctc gcccgggcgc acaagcgttc ggcagctcct ctcccggaca ggaatcgcca
     2341 cgtcgaatcg cgagcagtgt tcgatagtca ctgcccgcga ggtcgaacac gaagcgtcgc
     2401 tttcgattca gcgtgaggcg cagcatctgc tcgggagcac cgcgcgcaga gccaccgccg
     2461 tagtcgcagc cggcagagta atctgcggag gcattgttgg tgttaccctc gaagcgccca
     2521 ccgagctccg ggatgcgcca tgcctcctca caagtatccg agaacgatac gagcgtagtg
     2581 ggccccgcgg ggcgcacgaa ggcactcacc gaaac
//
LOCUS       DASTKU010000718         4305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_806487, whole genome shotgun sequence.
ACCESSION   DASTKU010000718 DASTKU010000000
VERSION     DASTKU010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806487"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VFQ61_25130"
     CDS             complement(<1..1496)
                     /locus_tag="VFQ61_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET9957815.1"
                     /translation="MNHMARALRFRSLSLGISLWLTTGWVGAHEEVEAPRLLGEPYAT
                     WPSGHELSTDLVVPVVMLVNAEGAVESVELEASVGEPFDSAAREAALAFRFEPARDAN
                     GARAAKVRAIVRFVAVHSLSPPPLAAPLSATSNPSPAEDTPRPPLTPLAPDGERERTV
                     TVRGQAPPRGASESELDRALLSAAPHRNASELLQTVPGVFVSQHSGEGKAHQIFLRGF
                     DAVHGQDVEMWAGGAPINDVSNLHGQGYADAHFLIPELVLRLRSTPGNYDPRQGDFAV
                     AGTLRFDLGYSEPGITAKASAGSFGARRFFMGYRPTQATDETFAAFELYKTDGFGPSR
                     AARHATAMAQLRHELTSAVTTRVLVSAYAGRFDSPGVLRLRDIESKNVDPFATYDAKQ
                     GGSSARAQLVWEIERATDAEAANPNQPQEHWSVAPFLVFRSLNLRSNFTGTLRSPEGD
                     STQQLNRATTLGATASYTRSVNWFSRNDRLEAGLYLRSDRIHQTQHRLS"
     gene            1932..2906
                     /locus_tag="VFQ61_25135"
     CDS             1932..2906
                     /locus_tag="VFQ61_25135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957816.1"
                     /translation="MLSNHRICLLALLSAALGAACKSTPAGTSNCAAAPQQSPIGAPC
                     PGFGAPVQTGSIDASNLLEISGIAASRTVPGAFYVHNDSDDGPRFYAIDASGRLLAAF
                     ELTGAAAVDYEDIAVGASNGRTFVYIGDTGDNGARDGSVAPRGEIQVYRVPEPNFPRV
                     PDLAQYAIGFERLRLSYPDRPHDSEALLFDSIRSQLILISKEDRGVATVFEVPPDVTP
                     DTPTTLVPRGPVVIGSCNGPTTGVVTGADISPDGRNIVLISYGLISLWQRSVTESVAD
                     ALGRPAQFTATLGAPNAEAITFAPDGRSWYATTEGANAPLLKATLNCP"
     gene            complement(2983..3717)
                     /locus_tag="VFQ61_25140"
     CDS             complement(2983..3717)
                     /locus_tag="VFQ61_25140"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9957817.1"
                     /translation="MSSKTLDDGLRVRERGEPGMAARGWGGQRRGAGRKKQRGLESVP
                     HEPRAPVRPQFPRHVTLCLNTRALRTQSVFNAVCAAFRALHRKWGESFRVAEFSVQSD
                     HIHLLIEANTDAALRSGMKSLGARLSHGINRVLGRRGKLYRDRFHDRELTSARAVRVV
                     LAYLFANFRKHQTKEALAGLSRDPFSSAPYFHGFVSADFDTPFAGHGPPEANLPIMRA
                     RGRLLSRDWQRHGLLSVGYWPPARVA"
BASE COUNT          818 a         1408 c         1319 g          760 t
ORIGIN      
        1 gagagccggt gttgggtttg atgaatgcga tcagaacgca agtagaggcc ggcttccaag
       61 cggtcgttgc gggagaacca gttgacggag cgcgtgtagg aagcggtggc gcccagcgtc
      121 gtggctcgat tcagctgttg agtcgagtcc ccctccgggc tgcgaagcgt gcccgtgaag
      181 ttggacctca gattcaggga gcggaacacc aggaacgggg cgacgctcca gtgctcctgc
      241 ggttgattcg gattggccgc ctcggcgtcg gttgcacgct cgatctccca gaccaattgt
      301 gcccgggccg agctgccccc ttgtttggca tcgtaagtcg caaacggatc aacgtttttt
      361 gactcgatat ctctgagccg caagacaccg ggggaatcga agcgtccggc gtaggcggag
      421 acgagcactc gagtggtaac agcgctcgtg agctcgtgcc tcaattgagc catggccgtg
      481 gcgtggcgcg cggcgcgtga ggggccgaag ccgtcggtct tgtagagctc gaaggccgcg
      541 aaggtctcgt cggtggcttg tgtggggcga taacccatga agaagcggcg agccccgaag
      601 ctcccggcgc tggccttggc cgtgataccc ggctccgagt agccgaggtc gaaacggagt
      661 gtgcccgcca ccgcgaagtc cccctgccgc gggtcgtagt tgccgggggt ggagcgcagc
      721 ctcagcacca attcgggaat tagaaaatgt gcgtccgcgt agccctggcc gtggagattc
      781 gagacgtcgt tgatgggcgc gcccccggcc cacatttcga cgtcttgccc gtgcaccgcg
      841 tcgaagccgc gcaagaagat ttgatgtgct ttgccctctc cgctgtgctg gctcacgaac
      901 acccccggca ccgtctggag cagctcactc gcattgcggt gcggagcggc cgacaacagc
      961 gcgcggtcca attcggactc tgaggcaccg cgcggcggcg cttgcccacg cacggtcacc
     1021 gttcgttcgc gttcaccgtc tggcgcgaga ggtgtgaggg gcggtcgcgg tgtgtcttcg
     1081 gccgggctcg gattcgacgt agcagagaga ggcgccgcca ggggcggggg cgacaacgag
     1141 tgtacggcca caaagcgcac gatggcccgc accttggcgg cacgcgcgcc attcgcgtcc
     1201 cgagccggct cgaagcgaaa cgcaagcgcc gcctcgcgag cggcggaatc gaacggctcc
     1261 ccgacggaag cctccaactc caccgactcg actgcgccct cggcgttcac cagcatcacc
     1321 acgggcacca ccaagtcggt ggataattcg tgcccggacg gccaagtcgc ataaggctca
     1381 cccaacaagc gcggtgcttc cacctcctcg tgtgccccga cccagcccgt agtgagccac
     1441 aaactgatgc ctaggctcag ggacctgaaa cgcagcgcgc gagccatatg attcattgtg
     1501 ctactagttg aaccattcgt gtcacacaag ccctgcgacg cgacttcacg ctcccgccac
     1561 acagactcac tcgatgtgac cgaccaacgt cagatacgcc ctccacgcca cctcggatca
     1621 cctgattttt cttcggccac gagataaaga gcgcgacccg ccggcaatgc ctgcactcgt
     1681 gcgcagccgg cttctcccgc gcccccccaa gataccgcgc cggcccccgc ggtaacctaa
     1741 cgttgggcga tcgtgctacc cggcccggcg ggtttccgag cgcgcgtcga ttcaacgctc
     1801 ccgtgcctgc tcgagggctg ctttttgctt acgagctgcg catccgatca gctggtacgg
     1861 agctcctggg ccgcgaatgc atcgggttcc ttcgtctcgg tctggcgggc atgatgagcc
     1921 tccaccacgt tatgctttcg aatcatcgca tctgcttact ggcgctcctt tcagctgccc
     1981 tcggcgctgc ttgcaagtcc accccggcgg gtacgagcaa ctgcgcggcc gctccccagc
     2041 aaagcccgat cggcgcgccg tgccccggct ttggcgcacc ggtccagacg ggcagcatcg
     2101 acgccagcaa tttgctcgag atctcgggca tcgccgcgag ccgcaccgtg cctggcgcct
     2161 tctacgttca caacgattcc gacgacggcc ctcgcttcta cgcgatcgac gcgagcggcc
     2221 ggctcctagc cgccttcgaa ttgaccggag cagccgccgt cgactacgag gacatcgcgg
     2281 tgggtgcgag caacggccgc accttcgtat atatcggcga caccggcgac aacggggccc
     2341 gcgacggctc cgtcgccccg cgcggcgaga tacaggtgta tcgagttccg gagcccaact
     2401 tcccacgagt accggacctg gctcaatacg caatcggatt cgagcgcctt cgcctctcgt
     2461 acccagatcg cccgcacgac agcgaggccc ttcttttcga ttccatacgc tctcagctca
     2521 tcctcatcag caaggaagac cgtggtgtgg cgaccgtgtt cgaggttccg cccgacgtga
     2581 cccctgacac gccaacgacc ctcgtcccac gcggtccggt agttattggc agttgcaacg
     2641 gccccaccac gggtgtggtc acaggcgcag acatttcgcc cgacggccgc aacatcgtcc
     2701 tcattagcta tggattgatt tctctttggc agcgctccgt caccgaaagc gtcgccgacg
     2761 ccttgggtcg tcccgcgcag ttcaccgcaa ccctcggcgc ccccaatgcc gaagcgatca
     2821 ccttcgctcc tgacggtcgc tcctggtatg ccacgacgga aggcgccaat gccccgctgc
     2881 tgaaggcgac tctcaattgc ccgtgaactc aatgcgctcg atttaggatg tggcgccgat
     2941 gcgcaaaggg agccgggcga aggacgagcg actccacagc ggttacgcca cgcgagcggg
     3001 cggccagtac ccaacggaca acaacccatg gcgttgccag tcacggctca gcagccgccc
     3061 ccgagcccgc atgatgggca ggttagcctc aggaggcccg tgacccgcaa aaggggtatc
     3121 gaaatcggcg gacacaaaac catggaagta aggggctgag gaaaaaggat ctcgcgaaag
     3181 ccccgccaga gcctctttcg tctggtgttt gcggaagttc gcgaagaggt atgccaacac
     3241 gactcgaacg gctcgcgccg aagtgagctc gcgatcgtga aacctatccc gatagagctt
     3301 acccctgcgc ccgagcaccc gattgattcc gtgcgacaag cgcgccccca gcgacttcat
     3361 cccgcttcgg agtgcggcat cggtatttgc ttcgatgagc aggtgaatat ggtccgattg
     3421 gacggagaac tccgccactc gaaagctctc gccccacttg cggtggagag ccctgaatgc
     3481 cgcacaaacg gcattgaaga cgctctgcgt gcgaagcgct cgagtgttca ggcacaaagt
     3541 aacgtgtcgg ggaaactgcg ggcgcaccgg agcccgcggt tcgtgcggaa cactttccaa
     3601 tccgcgctgc ttcttccttc cggcgcccct cctttgaccg ccccaccccc gcgctgccat
     3661 gccgggctcg ccccgttccc gaactcgcaa tccgtcgtct agagtcttcg aactcatggg
     3721 atctccaagc agtttgtcga gcttgacctt tcgagagcat ctgaagccgc gatgacacgc
     3781 ggcatagcgc gagtaacaac agccccacgc ccgataagct tttgggcctt acgctaccgt
     3841 accgccttac taaggcgaca tcgagagtct cgagcgatta cggtagcgtc agtcctcact
     3901 cgcgtctcag gagtcgaaac aacaacatac cacacactct gtttgctacc cgtctggcat
     3961 gtggctcagc cgcttatgca ttgatcggac tcgtcgtggg tcgggaggag cacctctgga
     4021 cgatcgacgt cctcgcagtg tgcgccgtcc gaaggtggag gaggcaggga gtggtccgaa
     4081 ggggccacgc ccaaaccaaa agaagaagaa gaagaagaag aagaagaaga agaagcgcgg
     4141 cgcctcgaac tcatgaggtg aggacgcgat ggggcacgct gcgagacgct agtgcaccgc
     4201 atgtgagatg cgatcaagca ttcggcggtg gctgcggatg atagcggcag cgggcttggt
     4261 ccagatgaag ggcgtcgggt ccttgttcca cccggcgatg tagga
//
LOCUS       DASTKU010000719         6175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_806568, whole genome shotgun sequence.
ACCESSION   DASTKU010000719 DASTKU010000000
VERSION     DASTKU010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806568"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(565..852)
                     /locus_tag="VFQ61_25145"
     CDS             complement(565..852)
                     /locus_tag="VFQ61_25145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957818.1"
                     /translation="MGAARGQLAHAVATVALQISTTNCLAVEVAALRRAIVSVLARAV
                     KVGADYKKFPKTWLFEHRWGGRRGSEQIAGQRIVREEVGGRTTAWVPPRQK"
     gene            2644..3363
                     /locus_tag="VFQ61_25150"
     CDS             2644..3363
                     /locus_tag="VFQ61_25150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957819.1"
                     /translation="MNGLIAVSFLFLIASSCSPKDDGAAVSAGGASTTVGSGGFTGGI
                     GGSSSVASGGLDFGGNTVEPIPPRECKPSAAKPTFRLTGTICGKAVEYSAPEGTRILI
                     SHAPFVSDVNVFTVADTEFATTPNTLSRDFLKTHDVQFHVNTNESMAFAVGESTLTVH
                     GGKIHICGIGTAFFKEETQMKAAIESIEPESNARGRIRVRLSNLRVDAADSEFGATEV
                     PVCSTEGELTAVFEGIYERVI"
     gene            complement(3595..4047)
                     /locus_tag="VFQ61_25155"
     CDS             complement(3595..4047)
                     /locus_tag="VFQ61_25155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957820.1"
                     /translation="MWRRLHSDQRWERPLLLEGVAGLATPVGFAGVMVESSTPFFAAS
                     AGTGLGFTGIQLAAQARLRLPLGGVAPYAGLGFSVGPFARLRPFSCCSGDPTLFMWLN
                     TELGVEGRDKAGTNVRVFVGLGQSVDPVVLPYVGVAVGHAFSFEPKSS"
     gene            complement(4271..5116)
                     /locus_tag="VFQ61_25160"
     CDS             complement(4271..5116)
                     /locus_tag="VFQ61_25160"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET9957821.1"
                     /translation="MPELPEVETACRTIRHALQGQRITHVEVVRDSLVFGTATPKTIE
                     KALLGRTLKAVGRRGKFFWLGLSGEGPTLFGHLGMSGWIREVGLEGVRLHGHGDAPFD
                     DEHGRPRFLKLGIAARSGRGIVLTDPRRLGRVWLGPAPEQDRRVKRLGPDAFEALPSA
                     KELLELFSRRKIPIKAVLLDQGALAGIGNWIADEVLYQSRISPKRAAASLTAEEVADL
                     RRAIVSVLARAVKVGADYKKFPKTWLFEHRWGGRRGSEEIAGQRIVREEVGGRTTAWV
                     PTRQK"
     gene            complement(5197..5718)
                     /locus_tag="VFQ61_25165"
     CDS             complement(5197..5718)
                     /locus_tag="VFQ61_25165"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9957822.1"
                     /translation="MSEQTHAEILEGEIVIRPSPTPAHQSASAELYAELRAPFQRGRG
                     GPGGWWLIPDVDVEFGPYDICRPDLTGWRREHLPDFPATRPIRTRPDWICEALSPGTA
                     LRDQGYKRDLYQRAGVPWYWMLDPVNRTITVLRWIREGYVLDHVAGDAGKAALPPFEG
                     FEIELETVFPPRE"
     gene            complement(5768..>6175)
                     /locus_tag="VFQ61_25170"
     CDS             complement(5768..>6175)
                     /locus_tag="VFQ61_25170"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9957823.1"
                     /translation="CAFAGGRLPEAAEWRFAASGLQGRRFPWGFTGLVCRRASFGLLH
                     GPCSDVGTGPDLVGARPDGNTPEGIADLSGNVAEWVKEPNGTYRARGGSYRSRVAVEL
                     LTAAVETRPPNAADVGFRCVYPEEPEETAEGPR"
BASE COUNT         1217 a         1910 c         1880 g         1168 t
ORIGIN      
        1 gtgtcccagc gtaacgccta caaagggctg gccaaaggaa tggccgacgt tttgagcgta
       61 tcccgacgaa gagccgaacg ttggtccccg cttggtgccg gaattcggcc ccgagtttcg
      121 cgttgagcca cgtggcttcg ttcgtgcagt accccagcgt caaaacttgc gagctcccct
      181 cccaatggga gaaccgcggt ggtgtggcag catcagggtc ctgcagcgtt caatcgccct
      241 ttgggaggaa aatcgtgacc agttcgcaac ctatcgaccc ggagaccccg cagtagtgct
      301 ctaccgagcc ttgtatcccg atccgtccgt acaatccgaa aacccagcgt gtatgtcttg
      361 tgcagaccat ttgggttcaa gtgacgagat gaacagtctt attgcggtaa gttacttggg
      421 ttttgattgt tgcctcgtgt tctccgaagg atgatggcgc agcgtcaagc gcaggtggcg
      481 catccacaat agccggcagc gctgggttca agggtgcacg aactttaacg cgtccagcct
      541 gcccggagca cttgggaggg tgagctactt ctgccgtggg gggacccacg ccgtcgtgcg
      601 tccgccgact tcttcgcgaa cgatacgttg ccctgcgatt tgctcggagc cgcggcggcc
      661 gccccagcga tgctcgaaca accaggtctt ggggaatttc ttgtagtcgg cgccgacctt
      721 cacggcccgc gcgagcaccg agacgatcgc gcgacgtaag gcagccactt ccaccgcaag
      781 acaattggtt gtagagatct ggagcgcgac ggtcgcgacg gcgtgcgcga gttgaccgcg
      841 agctgcgccc atccccagat gccccggcga acgaagagcg caaggccgct tcacccctcg
      901 agcgggagca caccgctcag cgccgccgtg cgcacctctc gtacggctcg gctaggaccg
      961 atatcgcagg ttggacgctc acgagcttgc tgcggcacgg aataaataac caaccagtcc
     1021 ggcgcaaaga ctcgaacagt gtaaccactt gggtagttgc tccgctgcgt cgtcaatgcg
     1081 cagctaaact tgctccccta tgagcgacat catccagttg acgtaacgca agctcgagtt
     1141 cactaacccc caaggtgaat caaagagacc tgattgacct cagactaaag ccggaacgcg
     1201 gagacaattg aattgctttg cgcgggtcgt tcttgatagg caactcaggt agtttcagtt
     1261 gcgataatct tgggagacca ggggagtatg acgcaaagga cggaacgtcg gggtttggtc
     1321 ggaggcgtgc agctgtggcg ctgcttgttc gcgtcgatac tggcgctctg tatactggcg
     1381 gcggggtgca cgaccaatgc tggttcagac gagcacgaca acgttgcggt tcaccgaggg
     1441 gcactaagtg ttccactctc gattcggatc cagcgactcc ctctcgggct cctaggtgcc
     1501 tagacgacaa ttggtcatga tcgctcggat ggtccctccg tcatcggtgc tatcgcgact
     1561 aactggcacg ctctcctgga cgggcactat gttaatggcc ctcttctgaa ccccggttcg
     1621 ggcaagcaat accttcgaac gttgaagcag tcaattcccg gcgctagcgc ctcgagcctc
     1681 ctatatacgt cggatgcgta tggaaacctt gaggacgagc cgcttgctct tcccggctgg
     1741 aagatgagaa acgtggttga cgagttcctt agccgcacag aggatttggc cgtgccgaac
     1801 ccgtctgccg cgctgaaccc aatacactca ggacggcgat ggcgtatacg gcgcgcggct
     1861 cggcatcctg gtgcgatgcc tgccgggtca tggctctcca tgagccgggg cacaggggag
     1921 cgctgggact tgggatgcaa ccctcagtct gaacaccacc gggtgcagcg gggttcgtca
     1981 gtcacgcgga tactcgcatc cgcgactccc aatggcagaa cctgcagcgt tcaaccgcct
     2041 ctctaggagt actgtgaccg ttccgaacag attccggcca agacgcttcg gcgcttgtgg
     2101 ggctgtaggg ggcttcctag tggtccttca gagttgcgat tcagttgact gctggagcct
     2161 ggaacaggga cacacctaca acttcacggt gtatgaagca ggaccgcaga gcgcacgcag
     2221 accatacggg tacccctgcg atcctcaatt gccgccgctc cttggagaca cttttcagtt
     2281 gaaggtagtt ggtgtgcgag acgatagtag gccgggagac gactgcaaag agtccttcgg
     2341 actgctgctg ggagggtacc cttcctcaag catggaggaa agttcgtctc ttcccgcagc
     2401 tgaagtcccg acctattttg ctgttagcca agagctcatt cgcgcgtcgg cacgaattag
     2461 gtggaatgga tgcaagggcg tctggtcgat cattgctctc ggaaattcgg gaagtcatga
     2521 cccattctca gcctatcgcc ccggagaccc cgcagtagtg ctctaccgag ccttctaccc
     2581 cgacccgtcc gaaaacccag cgtgcgtgtc ttgtgcagac cattttggag ttcaagtaac
     2641 gagatgaacg gtcttattgc ggtaagtttc ttgtttttga ttgcttcatc gtgttctccg
     2701 aaggatgatg gcgcagcggt gagcgccggt ggcgcatcca caacagtcgg cagtggcggg
     2761 ttcacgggtg gaatcggcgg ttcaagcagc gtggcctctg gtggccttga ctttggcggg
     2821 aacaccgtgg agcccattcc tccccgtgag tgcaagccta gcgcggccaa gccaactttc
     2881 cgactgactg ggacgatctg tggaaaagca gtcgaatact ccgcgcccga ggggactcgg
     2941 attttgatct cccacgcacc cttcgtgtca gacgtgaacg tgttcacggt ggcggacacg
     3001 gaattcgcaa ctacgcctaa cacgctttcg cgagacttct tgaagacgca cgatgtgcaa
     3061 tttcacgtca acacgaatga gagcatggct tttgccgtag gcgagagcac gttgaccgtt
     3121 catggcggga agatccatat ctgcgggata gggaccgcct tcttcaaaga ggaaactcag
     3181 atgaaggctg ccatcgaatc gatcgaaccg gagagcaacg cccgcggacg cattcgtgtt
     3241 cggctctcca atctccgcgt ggatgccgcg gactctgagt tcggcgcgac ggaggtgccc
     3301 gtgtgcagca ctgagggtga gctgaccgcc gtcttcgagg ggatatacga acgcgtcatt
     3361 tagaacctat cccagtagca gcgtcggaga cgggggctga gaactatcgg ccgctggcga
     3421 ctagctccta ttggggcagg ttcttagcca tgagatggag cgcccgcctg agttagaaga
     3481 acaacgccca gctcgcaccc gcgctgagca cgatcgcatg gtaatgttcg aacgaagatt
     3541 ctcgccgata agtcggagat gagtcgcagg tttgccccct gcacagccac tgatttacga
     3601 ggacttaggc tcaaacgaga aggcgtgtcc caccgccact cctacatagg ggagaacgac
     3661 agggtcgaca ctttggccca agcccacgaa gactcggacg ttcgtgccgg ccttgtctcg
     3721 gccctcgact ccgagctccg tgttgagcca catgaacagg gtcggatcac ccgagcagca
     3781 gctgaaaggg cgaagtcttg caaacgggcc gaccgagaag cccagtcccg cgtacggcgc
     3841 gacgccgccc aacggcaaac gaaggcgcgc ctgcgccgcg agctggatgc ccgtgaatcc
     3901 gaggccagtg ccggcagaag cggcaaagaa tggggtggag ctttccacca tcacgccggc
     3961 aaaacccaca ggggtagcta gcccggcaac tccctcgagc agcagcgggc gctcccaacg
     4021 ctgatcggag tgaagcctgc gccacactgt ccctggcggg agacggtcgc gggctcgcgg
     4081 ccggggactc tcggggctgg ataggtgcct gttgagaagg cgggatttgt ggggcgggct
     4141 gtggaacggc ggcgccggtc aattgagcca gcgccaacca actctagagc atcatgcgga
     4201 cgcaaacagc acgccatgag ccgcgaagtc tcggcgggtc ctgcctgtcg ggagcccgtg
     4261 tgatggtgag ctacttctgt cgcgtcggga cccaggccgt cgtgcgtccg ccgacttctt
     4321 cacgcacgat gcgctgtccg gcaatttctt cggagccccg gcggccgccc caacgatgct
     4381 cgaatagcca ggtcttcggg aatttcttgt aatcggcgcc gaccttcacg gcccgcgcga
     4441 gcaccgagac gatcgcgcga cgtaggtccg ccacttcctc cgcagtgagt gaagccgcag
     4501 cacgcttggg tgaaatccga gattgataca gcacctcatc ggcaatccaa ttgccaatgc
     4561 cggccagcgc accttgatcg aggagcacgg ctttgatcgg aatctttcgc cgtgaaaaca
     4621 gctcgagcag ctccttggcc gaaggcaaag cctcgaacgc gtccgggccc aagcgcttga
     4681 cccgccggtc ctgctcgggt gcaggcccaa gccagacccg gcccagtcga cgcggatccg
     4741 tgagaacgat gccgcgaccc gatcgcgccg cgatgcccag tttcaagaac cgcggccgcc
     4801 cgtgctcatc atcgaaaggg gcgtctccgt ggccgtggag gcgtacccct tccaaaccca
     4861 cttcgcgaat ccatccgctc attcccagat ggccaaacaa cgtgggcccc tcgccactca
     4921 aaccaagcca aaagaacttc ccgcgccgac cgacggcctt tagcgtgcga ccgagtagcg
     4981 ctttctcgat ggttttcggg gtggcggtcc cgaacacgag cgaatcacga acgacttcga
     5041 cgtgcgtgat tcgctgccct tgcagcgcgt ggcgaatcgt tcggcaagcg gtctcgacct
     5101 cgggtagctc gggcatgcgc ctgattgtag ccgcgaccgt gcgcgcggtg cccgtgaagc
     5161 ggccctcgcg tgcgggcacg ggccttgtgc ggcttttcac tcgcgcggcg gaaacaccgt
     5221 ctcgagctct atctcgaagc cttcgaacgg tggcaacgcc gctttgcccg catccccagc
     5281 tacgtggtcc aacacatagc cttcgcgtat ccaacgtaag acggtgatgg tgcgattgac
     5341 tggatcgagc atccaatacc agggcactcc cgcgcgctga tagagatcgc gcttgtatcc
     5401 ctgatcacgt aaggcggtgc ccggggaaag cgcttcgcaa atccaatccg gtctcgttcg
     5461 gatcgggcgc gtcgcgggga aatccgggag gtgttcgcgg cgccaacccg tgaggtcggg
     5521 tcggcaaatg tcgtaaggtc cgaactccac gtcgacgtca gggataagcc accagccccc
     5581 cgggcctccc cgtccgcgct gaaatggtgc ccgcagctcg gcatacagct ccgccgacgc
     5641 gctttggtgc gcgggcgtgg gcgagggccg aatgacgatc tcaccctcca aaatctcagc
     5701 gtgcgtctgt tccgacaaca gcaagaggtc cgagtagctc gctcgtcgtc gttccgagtc
     5761 tggcacttta ccgcggcccc tccgccgtct cctctggctc ttccgggtac acgcaccgaa
     5821 accccacgtc cgccgcatta gggggcctcg tttctaccgc cgcggtcagg agctcgaccg
     5881 ccacgcgcga acgataggac ccgccgcggg cgcgataggt tccgttcggt tctttcaccc
     5941 actccgcgac gttgcccgat aggtccgcga tgccttccgg cgtgttgccg tcgggccgcg
     6001 ctcccaccaa gtccgggccg gtgccgacat cgctacaggg gccgtgaagc aagccgaagc
     6061 tcgcgcgccg acacacgagg ccggtgaaac cccaggggaa gcgccgccct tgcaaccccg
     6121 aagccgcaaa gcgccactcc gcagcttcgg gcaaacgccc gcccgcgaag gcgca
//
LOCUS       DASTKU010000720         9511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1925507, whole genome shotgun sequence.
ACCESSION   DASTKU010000720 DASTKU010000000
VERSION     DASTKU010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9511
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925507"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFQ61_25175"
     CDS             complement(<1..212)
                     /locus_tag="VFQ61_25175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957824.1"
                     /translation="MSASSGVVSIEAIPPLLGRYQLSQYLDSGGTADVFRAVDLSDGS
                     TVAVKILNETARHDVRLRRYFLEGSRA"
     gene            complement(777..1130)
                     /locus_tag="VFQ61_25180"
     CDS             complement(777..1130)
                     /locus_tag="VFQ61_25180"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HET9957825.1"
                     /translation="MNAVAAGAIGLTLGVGLGAFGAHGLSHLGPVKLGWWGTATQYEV
                     MGSFGLIAVGLFERAPVSWRSPAGLLILGMLLFSGSLYAMALGAPRWFGAITPFGGLS
                     LMGAFAWFAIQAVQR"
     gene            complement(1326..3068)
                     /locus_tag="VFQ61_25185"
     CDS             complement(1326..3068)
                     /locus_tag="VFQ61_25185"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9957826.1"
                     /translation="MAQPVAQKTDRSSRGRRGEDPSLGKVIAGRYRLEARIGEGGMGI
                     VYRARHVLIDRVVAVKLIRPDLRGETHLRAWMLREARAANRVDHAHIIDIHDIGETDD
                     GELYLVMEYLVGIPLSSELARGPVPLARALDILEQMGAALTRAHDLGVVHRDLKSDNI
                     LLTQRGGRKDFVKILDFGLAALAHDPRLAPKGAVFGTPEYMSPEQARGEQAGPHSDLY
                     ALGVLFFEMLTGQLPFRASDRETLLEMQRTAPAPRPSSIKKDCHPVAERIVLKLLEKE
                     PRKRYRDGHHLLEELKALQRSQPNTSWEKENQESAPVAPPPPPLPKSPGVTEWGNRAG
                     LFGRMMARAYPSANAPPELSQALATLWEVAAKANALEGEVASHTRKLEALERRGRALR
                     AEIGRKVEELAQEESRVMREAAAYGEEEEAARRELARAQQVAQERVAQADQAERSGKA
                     TRAIFEQAGAAAAMVDAQRQWLKTRETRRTSREATAVDLRRQIDDLRAQLSRYAEALE
                     EDLAAGREKVAARTREGLKFEKAFQDASHVLIAQLRGKPECRELMNELMAADGIEPET
                     ASINVPLPRAAEAS"
     gene            complement(3182..3889)
                     /locus_tag="VFQ61_25190"
     CDS             complement(3182..3889)
                     /locus_tag="VFQ61_25190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957827.1"
                     /translation="MRIVPYVLTLPFLLTAFSAHAEEPSGSPKGIIGGALLGGEVVIA
                     AEAAFKVRSPWAYLGGGLAGAAAGGLAGHFIEDGASPRVPLVMLAAGLTLAVPTTVAV
                     LSATAYEPPADFVQDQGPTDEPVAEPPRPGSPTPGATPSPATGTPDAASEVPATTPPA
                     TTPTEPPRSERTRRTRAREATATRYMPPALVDIQPEKLSLSVPSVEVGQVFSRREIAM
                     GAPKATEVRIPLLNVLF"
     gene            4234..5142
                     /locus_tag="VFQ61_25195"
     CDS             4234..5142
                     /locus_tag="VFQ61_25195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957828.1"
                     /translation="MPIRRVILVYLAVFGVACMTAVLRLQAVDVSSAPSAAWLPFVYP
                     LLGAVSEMTLLLGVPSSVLCGLATGLSWRTGLACACALLALGFGLSGFAESASRAPGV
                     LAQELVDAGREQCLKSPSHRSDVPIVPVAWRCPSSKRTTLVGRGSWSSKIELEAGTLE
                     LAEDLREAKLSRVDAKLKARAPLPALHLKVSKVRVRGLPPWGRPANLSFAFRLALATC
                     AVTLTLGVGAFALRRERVPRLAVIACAFLSGAVVLGLELWLDRRLSSAASYVGAVFAG
                     PALCAIVLVIFERCAPRRSFVAQGRS"
     gene            5215..5475
                     /locus_tag="VFQ61_25200"
     CDS             5215..5475
                     /locus_tag="VFQ61_25200"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET9957829.1"
                     /translation="MFALLVVVLIVLLLLGGDRLATLGKGLGEGAKAFRKATHNPRPP
                     ARRVELHSVELRRPENEPASPKLLPAKGETSSEAHTPDSERH"
     gene            5481..6422
                     /gene="cyoE"
                     /locus_tag="VFQ61_25205"
     CDS             5481..6422
                     /gene="cyoE"
                     /locus_tag="VFQ61_25205"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET9957830.1"
                     /translation="MLPSSGSAELPSADAVPSSNSAGSLNAALPPKFNIYLELSKPGV
                     GGLVIVTTLCGALTAPGRVAFGRLLITLMGTALVVAGANAANMWLERDTDVFMRRTRT
                     RPLPSGRLAPEAALWFSGLTCVAGLALLVSCVGWLTAALAALAYVSYVAAYTPLKRVT
                     PLALFVGAVPGAIPPLIGWASARGELSTLGWLEFLVLFVWQLPHFLAIAIFRRDEYAR
                     AGLLVLPVVHGVRRTKIEILLYSAALVAVSLLPVGLGLTGLGYAAVAIACGLPFLVLA
                     SAGLSVADDRRWARRVFFASMPYLVLTLGALSIFSTR"
     gene            6562..6921
                     /locus_tag="VFQ61_25210"
     CDS             6562..6921
                     /locus_tag="VFQ61_25210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957831.1"
                     /translation="MASSILAGCWESLTLRPEDAPAPRDYYREIVRKKSDCSTDVPLL
                     REGEVNRPYEEVSRLSSTCYPGTPAVCERTLKERACQLSADALLVQRSVASGSPPGTS
                     HESLMAMQARAVRWTAR"
     gene            complement(7481..7861)
                     /locus_tag="VFQ61_25215"
     CDS             complement(7481..7861)
                     /locus_tag="VFQ61_25215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9957832.1"
                     /translation="MKILVLEDDPRIARCLQRELTRAGYQVCATRLCRTARAWNHKVD
                     VAVLDIELPDGSGVDIARELLAHGTARRVIFFSSARDQQLLRSAAEHGEVLTKSGDFA
                     PLITALASKSDVRLRRRRLGPARR"
     gene            complement(8223..9242)
                     /locus_tag="VFQ61_25220"
     CDS             complement(8223..9242)
                     /locus_tag="VFQ61_25220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957833.1"
                     /translation="MSVNTTPAGNEAAPTSTPETREVADWGLPLLGFAGLALCALVLT
                     KGFAQALPGSATGIERWIAITSFLGACASQVLAAGGIALCLRLLGNMLGLPGLGLAFR
                     ITMAPVGLLVVTLVAVAAARPLTPELGRLLALTAVFAQLSALPFLLPPRWLRGAGLVL
                     LASALSAILELIAYESSGGKAGGAQIMLVSALDLAARSLDAAAASLALIWVSHSARKA
                     GALHGLLVAVVIALLSLARMGAVHSAHPVLVVLRRSIASLVERPSLLQASWLGSALSL
                     FALLGAFALLSRRGQRPELRAALCLGLVARACAGAPVAALLSVGTALLLSSIALDPKR
                     APRAT"
     gene            complement(9264..>9511)
                     /locus_tag="VFQ61_25225"
     CDS             complement(9264..>9511)
                     /locus_tag="VFQ61_25225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957834.1"
                     /translation="NPVIACTTGNAHPFILKTKPKAAPAPVPTPAPPPADDERHGQLR
                     PRSPERARAPQPATPEKRRAVGGKHAPPARGELALSP"
BASE COUNT         1606 a         3067 c         3032 g         1806 t
ORIGIN      
        1 gctcgcgagc cttcgaggaa gtagcgtcgc agccggacgt cgtgccgagc cgtctcattg
       61 aggatcttta cggccaccgt gctgccgtcc gacaagtcga cggcgcggaa gacgtccgcg
      121 gtgccgccgc tatcgaggta ctggctcagc tggtagcgcc caagcagtgg agggatggcc
      181 tcaatggaga cgacgcccga agaggccgac acgcctgcag actctggcgt aggcgagaat
      241 atagctcgcc caggcgaata tcccgctcta gttacgtctg ttccgcaagt gaagcccata
      301 gccatgacgg gacttacctc tgccgcggaa aagatgcgcg gcagtactct aagccaccga
      361 atagcattgc tctgcgctcc ggtgacaact ttcgtgattg agtaactaat acaaccacac
      421 acccgaaccg tggtgatcaa ttcatagctt cagtgttttt ggagctacgt tggagttcgt
      481 ccgagcccga atttgcgcgc ccgaacgcaa ggcgtgctgc ccgtccccgc atgattcggc
      541 tggttcttag cgatgattcg gccggattcc aggcaaggcg gggctgagct cgatcaccca
      601 gacgagtgat cgagctgacg gccgttaggg tccagcgccc tttggcgctg caggtatttg
      661 cgggggcagc gctttcagcg ctgcaggtat ttggcggggg cagcgctttc agcgctgcag
      721 gttacttgcg ggggcagcgc tttcagcgct gcaggttctt tgcgggggca gcgctttcag
      781 cgctgcacgg cctgaatcgc gaaccaggcg aaagcaccca ttaggctcaa accaccgaac
      841 ggcgtgatgg ctccgaacca acgcggtgct ccaagggcca tggcgtacaa cgagccgcta
      901 aataggagca tccccaagat gagcaagccg gcagggcttc gccaggagac gggggcccgc
      961 tcgaacaagc ccacggcgat gagaccaaac gagcccatca cctcgtattg agtggcggtg
     1021 ccccaccagc cgagcttcac cggtccgaga tgagagaggc cgtgggctcc gaaagcaccc
     1081 aaaccgacgc ccaaagtaag gccaatggct cccgcggcaa cagcgttcat gtaggcgcgc
     1141 ggattctagc acctcgctca gcgtttccag ccgcctccca acgccatcga gctcgaactc
     1201 aggatgccgc tagggtcgcg tccagcactt cgtttgggct atgcgggtgc tctggcgagg
     1261 ccgcggtggc ccacttccag ccgcgcgttc cgggccttgg gccttcgcgc cggtgtcgag
     1321 ttgggttaac tcgcctcggc ggcgcggggt aggggaacat tgatcgacgc cgtctccggc
     1381 tcgatgccgt ccgcggccat tagctcgttc atgagctctc ggcattctgg cttgccacga
     1441 agctgagcga tcaaaacgtg cgaggcgtct tggaacgcct tctcgaactt caacccttcg
     1501 cgggtgcgcg ccgccacttt ttcacggccg gcagccaagt cttcctccag agcttcggca
     1561 tagcgcgata gctgagccct caagtcgtcg atttgccgac ggagatccac cgccgtcgct
     1621 tcgcgcgagg tgcgccgcgt ttcccgcgtc ttgagccatt gacgttgcgc atctaccatc
     1681 gccgccgccg caccggcctg ctcgaagatg gcgcgggtcg ctttccccga gcgctctgct
     1741 tgatcggctt gggctactcg ttcttgtgcg acctgctgcg cccgtgcgag ctcgcgccgc
     1801 gcggcctctt cctcttcgcc gtacgccgcc gcctcgcgca taacgcgaga ttcctcctgc
     1861 gcgagctcct ccactttgcg gcctatttcg gcgcgcagcg cgcgcccgcg tcgctccagt
     1921 gcttcgagct tgcgggtgtg gctcgccact tcgccttcga gcgcgttggc cttggccgca
     1981 acttcccaca aggtggccaa ggcttggctc aactccggcg gtgcattggc ggacggatag
     2041 gcgcgggcca tcatgcgtcc gaacaaaccg gctcgattcc cccactcggt caccccgggt
     2101 gacttcggca gtggcggagg aggtggcgcg acgggcgccg actcctggtt ctctttttcc
     2161 caggaagtat tgggttggga acgctgcaga gccttgagct cttcgagcaa atggtggccg
     2221 tcccgatagc gcttacgcgg ctctttttcg agcagcttga gcacgatgcg ctcggccacg
     2281 ggatggcaat ccttcttgat actcgaaggg cgcggcgcgg gtgccgtgcg ctgcatctcg
     2341 agtaatgtct ctcgatcgct tgcacggaat ggcagttgcc ccgtcagcat ctcgaagaac
     2401 aacacgccga gggcgtacaa atccgagtgc ggcccggctt gttctccgcg ggcctgctcg
     2461 ggcgacatgt actcgggcgt gccgaacacg gcgcctttgg gggccagacg cggatcgtgc
     2521 gccagcgccg ccaagccgaa gtccaggatc ttgacgaagt ctttgcgacc tccccgctgc
     2581 gtgagcagga tgttgtcgct cttcagatcg cgatgcacca caccaaggtc atgagcacgc
     2641 gtcagcgccg cacccatctg ctccaagata tctagtgcac gtgcgagcgg cacggggccg
     2701 cgcgccaact cactggagag cggaatgccc accaggtact ccatcaccag atacagctcg
     2761 ccgtcatcgg tctcgccgat gtcgtgaata tcgatgatgt gggcgtggtc cacgcggttg
     2821 gctgctcgcg cttcgcgcaa catccacgcg cgtaagtgcg tttcaccgcg taaatccggc
     2881 ctaatcagct tgaccgcaac cacgcgatcg atgagcacgt ggcgcgcgcg gtatacgatg
     2941 cccataccgc cttcgccaat gcgagcttct agccgatatc gtccggcaat gaccttgccg
     3001 agtgacgggt cttcaccccg gcggccgcga gaactccgat cagtcttttg cgcaacgggc
     3061 tgtgccatgg tgggtgcgct gaagcgcgct gtaacatcgc gcttcagcca aagatgctaa
     3121 cggctgactc gttgcaaagc aaagcccttt ccccgctttg aacaccgaga ccgttagcgg
     3181 atcagaacag aacgttcagg agcggaatgc gcacttcagt cgccttgggt gcgcccatgg
     3241 ctatctcgcg tcgggagaaa acctggccca cctcgacact cgggacggac agcgacagtt
     3301 tctctggctg gatgtcgacg agtgcgggag gcatgtaacg agtggcggtc gcttcgcggg
     3361 cacgggttcg gcgcgtacgc tcggacctcg ggggttctgt aggtgtggtg gcgggcggtg
     3421 tcgtcgcagg cacctcggaa gcggcatccg gagtacccgt agccggagaa ggcgtcgccc
     3481 ccggggtagg cgagccggga cgcggcggtt cggcgacggg ttcatccgtg ggaccctgat
     3541 cctggacgaa atccgccggg ggctcgtacg cggtggcgct caacactgcg accgtggtcg
     3601 gcactgccaa cgtcagaccc gccgccagca tcacgagcgg aacccgcggc gacgccccgt
     3661 cctcgatgaa gtgcccagct aggcctcctg ccgctgcgcc ggccaaacca ccacccaaat
     3721 aagcccacgg ggaacgcact ttgaaggcag cctcggcggc gatcacgacc tctccaccca
     3781 gtaaggctcc accaatgatg cctttcggag acccagaggg ttcctccgcg tgtgcagaga
     3841 aagccgtcag cagaaaggga agagtgagga cgtaaggaac gatgcgcatc gatacctctt
     3901 tcaaggtgag ccggaggcat acgttgcaaa gcctccctca gacattcgcc gcggcgagca
     3961 tagcatcgat gccgtccgtc cttacaatcc gcctcgagct gggggctgcg caatcctcga
     4021 gccgtgccca attcagcgtg ccattcggcc gtgccggtgc gttcggccgt gcccgtcgcc
     4081 ggaaccgccg cgcggccggc tcgccgaggt ttcggggtaa ttcgcccttt cgaacgaagc
     4141 cctccaacgc tctcgcaccg aaaagaacta ggaagagtac tgggcagcac gtcgttctga
     4201 ggcgacgccc tagggtgagg taggattccg cgggtgccga ttcgacgggt catcctcgtc
     4261 tatctcgccg tcttcggcgt tgcgtgcatg accgctgtgc tgcggctgca ggccgtcgac
     4321 gtatccagcg cgccgagcgc tgcgtggctg ccgttcgtct accctctgct cggggccgtg
     4381 agtgagatga cgctgctgct cggggtgccg tctagcgtgc tttgcgggtt ggcaactggg
     4441 ctctcttggc gcaccgggct cgcttgcgcc tgcgcgctcc tcgctctggg tttcggactg
     4501 tctggtttcg cggaatccgc gagtcgagca cccggtgttc tcgcgcaaga gctcgtggat
     4561 gcggggcgcg agcaatgctt gaagagccct tcacaccgat cggacgtgcc gatcgtgccc
     4621 gttgcgtggc gctgtccttc gagcaagaga accaccctcg tggggcgcgg cagttggagc
     4681 tccaagatcg agctcgaggc cggtactctc gaactcgccg aagatctccg ggaagcaaaa
     4741 ctgagccgcg tcgatgccaa gttgaaggcg cgtgcgccct tgcccgcgct gcacctaaag
     4801 gtttccaaag tgcgcgtacg cggcctgcct ccttggggaa gaccggccaa tctttcgttc
     4861 gcgtttcgtc tggcgctcgc gacgtgcgcc gtgacgctca ctctcggggt tggcgcgttc
     4921 gccctgcggc gcgagcgcgt accgagactc gccgtgatcg cctgcgcgtt tctgagtggg
     4981 gcggtcgtgc tggggctcga gctttggctg gatcgacgcc tgtctagcgc cgcaagctat
     5041 gtcggagctg tttttgccgg ccccgcgctc tgcgcgatcg tcctagtcat tttcgagcgc
     5101 tgcgccccac gacggtcttt cgttgcccag ggccgctcgt agtggtaacc aaggccgcgc
     5161 tccggacctc catccggccc ggcccgcggc tcctgcgcat gttggccatg cttcatgttt
     5221 gccctcctgg ttgtcgtctt gatcgtgctc ctgctcctcg ggggcgatcg tcttgcgaca
     5281 ttgggcaagg gcttgggtga aggtgccaag gctttccgca aagccacgca caacccgcgg
     5341 ccgcccgcgc gccgagtgga attgcacagc gtcgagttga gacgccccga gaacgaacct
     5401 gcctcgccga agctgcttcc cgccaagggc gagaccagct ccgaagccca tacccccgac
     5461 tctgaaaggc actgaacccc gtgctcccat cttctggatc tgcagagctt ccgagcgccg
     5521 acgcagtgcc gtcctccaac tctgcgggct cgctgaacgc ggctctgccg cccaagttca
     5581 acatttacct ggagctctcg aagccaggcg tcggcggttt ggtgattgtg accacgctgt
     5641 gcggggcttt gaccgcaccg ggtcgagtgg cgtttgggcg ccttttgatc acgttgatgg
     5701 gtaccgcgct ggtggtagca ggcgctaacg ccgcgaatat gtggctcgag cgagacacag
     5761 acgtcttcat gcgtcgcacc cgcacgcgcc cgttgccctc gggtcgcttg gcgcccgaag
     5821 ccgcgctgtg gttttccggg ctgacctgcg ttgctgggct cgccttgctc gtgagctgcg
     5881 tcggttggct cacggcggcg ctcgcggcgc tcgcgtacgt gagttacgtg gccgcgtata
     5941 cgccactcaa gcgagtcact cccttggccc tgttcgtggg agcggttcca ggcgccattc
     6001 ctcccctgat cggttgggcg agcgcgcgcg gcgagctgtc gactttgggc tggctcgagt
     6061 tcctcgtcct gttcgtctgg cagctcccgc atttcctcgc gattgcgatc ttccgtcgtg
     6121 acgagtacgc gcgagcaggg ttgttggtgc tgccggtcgt gcacggcgtg cgtcgcacga
     6181 aaatcgaaat cctgctgtat tctgcggctc tggtggcagt gagtcttttg cccgtggggc
     6241 tcggcctaac gggattgggc tatgccgccg tggcgatcgc gtgtggtttg ccgtttctgg
     6301 ttttggcgag cgcgggcctg tcggtcgccg atgatcggcg ctgggcgcgg cgtgtgttct
     6361 ttgcctcgat gccatacctg gtgttgaccc tgggcgcgct ttcgatcttc tccacgcgct
     6421 gagcaaggct atgcttgcac cagaatggct ggtgcgaacg tgcccaaacg atccagctgc
     6481 gcggctcgcg cgtttcgagt cgcaagacca tcagagggtc gtcgtcaggc ttcggccgcg
     6541 ctgatctatg cgacgctgct gatggcatcg tcaatactcg ccggctgttg ggagtcgctc
     6601 acgctgcgtc ccgaagatgc gccggcgccg cgcgactact atcgagagat cgttcggaag
     6661 aagagcgact gcagcacgga cgtgccgctg ctccgagaag gcgaagtgaa tcggccgtac
     6721 gaggaagtga gccgcttgtc gtctacgtgc tatcccggca ctccagcggt ttgcgagcgc
     6781 acgctcaagg agcgagcttg tcagctctcc gcggacgctc tgctggtgca acgctccgtc
     6841 gcgagcggct ccccaccggg cacctcacac gagtccttga tggccatgca agctcgagcc
     6901 gttcgctgga ctgctcgttg atgtgctgaa gcgctggacc aggccgggcc cgcggggtga
     6961 acgacaaaaa ggcaacggtg ccaagcggga ggagacgcct ggcaccgtcg aggagtttaa
     7021 ttgctggcgg ccatcctctg ggggaggggg gcaccctgag gtgccgccag cggttggaca
     7081 ctagtgcagt cgtcgtgcca agcgccgcga gggcgcgaac gcgagcgtct acgcgtccgc
     7141 cgcaccgatg gggagctctc tttgggctga tttgccgagg aatacacggt gcgcttcgag
     7201 tcgaggcgtt gcccgtcgcc gtccgatcac gagacgggcg agccagttcg agatctcgtt
     7261 tcgagctttc tcggtaagag atgtgcgaat tcgcaattcg ctgattcaaa tccgcagtgc
     7321 acactcttgt aagtgattga actgcgttgc gtgcgcgccg gggccactcg ctactcctgt
     7381 gggtgattga gctccggcct gtgccatcgc tgcgcgattc accatcgggt tcgagcctgc
     7441 gcgcgtctgt gtgtacactt cacggccgcg aacggcgcca tcagcgccga gcgggaccca
     7501 aacggcgccg acgtagccgt acgtccgact tgctcgcgag cgccgtgatc aagggggcga
     7561 agtccccgct cttggtcaag acttcgccgt gctcggcggc gctgcgcagg agctgctggt
     7621 cccgtgcgct gctgaagaag atgactcgcc gggcggtgcc gtgcgcgagg agctcgcgag
     7681 cgatatctac tccgctgccg tccggtagct cgatatccag cactgcgaca tccaccttgt
     7741 gattccaagc gcgcgcggtt cgacacaagc gcgtcgcgca tacctggtag ccagcgcgcg
     7801 tgagctctcg ctggaggcag cgcgcgatac gtggatcatc ctccagtacg aggatcttca
     7861 aaccaggatc cctcgactcc gcgtctggtc gaaactgcca attccgtgac agccgaggcc
     7921 cgctgacttg ccgatgactc tcgctcgatg gctgaagctg gcggtcttca tccagccgta
     7981 tgtcttcgcg gcgcgtgttt ccgcagcggg tctcgatgcg ccgtatttcc ccctgcgaac
     8041 gctggccttt ctgttcccgt gtatgggcca tggatcggcc cggaccatat cagcagcggt
     8101 tcagctgaac aagcgaatgg tgtctttcgt tccggacgat tcggaactcc agtatttcat
     8161 ttcaatacac gagcatcgaa gtcgtgatgg tcaatacacg agcatcgaag tcgtgcgcgc
     8221 actcacgtcg cgcgcggtgc ccgcttcggg tcgagcgcga tagacgacaa caacagtgcg
     8281 gttcccacac tgagcaaagc cgcgaccggg gcgcctgcac aagcgcgcgc caccagaccc
     8341 aagcacaggg ccgctcggag ttccggccgt tggccgcgtc tcgacaacaa cgcaaacgca
     8401 cccaacaacg caaacagcga aagtgcactg ccgagccaac tcgcttggag caaactcgga
     8461 cgctccacga gggatgcgat cgagcggcgc agtacgacca agacgggatg ggccgagtga
     8521 accgcgccca ttctcgcgag cgaaagcagc gcaatcacga ccgcaaccag caaaccatgc
     8581 aacgcacccg ctttgcgcgc gctgtgactg acccaaatta gcgccaagct cgcagccgcc
     8641 gcgtccaagc tccgggcggc cagatcgagc gcagacacga gcatgatttg cgcccctccc
     8701 gctttgccac ccgaagactc gtacgcaatc agctccagga tcgccgacaa cgcgctcgcg
     8761 agcagcacca accccgcccc gcgcaaccaa cgtggcggaa gcaagaacgg caaagcggac
     8821 agctgcgcga acacggcggt gagcgccaat agacgcccga gctcgggcgt cagcggtcgt
     8881 gccgctgcga ctgccaccaa ggtcaccacc aacaaaccaa cgggcgccat ggtgatgcga
     8941 aacgcgagcc ccaatcccgg cagccccaac atattgccga gcagccgcag gcacagcgca
     9001 atgcctcccg ccgccaaaac ctgacttgca caagcgccga ggaagctcgt aattgcaatc
     9061 caacgttcga tgcccgtagc cgaacccggc aaggcctgcg cgaaaccttt ggtcaacacg
     9121 agcgcgcaca acgcgagccc cgcaaagccg agtagcggca agccccagtc cgccacttcg
     9181 cgagtttctg gcgtgctcgt tggcgctgcc tcgttgccgg cgggggtcgt gttgacgctc
     9241 atgggcttcg tactcggtca ggctcagggg ctcagcgcca gttctccgcg cgccgggggt
     9301 gcgtgtttgc ctcccaccgc gcgtcgcttc tctggggttg cgggctgcgg cgctcgagcg
     9361 cgttcgggag acctcggccg cagctgacca tgtcgctcgt cgtcggcggg cggaggggcg
     9421 ggggtgggaa ctggagccgg tgccgctttg ggcttcgttt ttaggatgaa cgggtgcgca
     9481 ttgccggtcg tgcaggcaat caccgggttt c
//
LOCUS       DASTKU010000721         3668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1701257, whole genome shotgun sequence.
ACCESSION   DASTKU010000721 DASTKU010000000
VERSION     DASTKU010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1701257"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFQ61_25230"
     CDS             complement(<1..402)
                     /locus_tag="VFQ61_25230"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:sodium antiporter"
                     /protein_id="HET9957835.1"
                     /translation="MIQWIQLLGGGLLLYFGAEWFVGGASALALALRVPQILVGLTVV
                     AYGTSAPEIIVGIEAAGTGHGEVALGNVIGSNVANIGLILGLAVLVRPARVDGALRKR
                     ELPVLALSTALLPLLLLDGVISRVESGALVIF"
     gene            complement(429..1850)
                     /gene="nhaA"
                     /locus_tag="VFQ61_25235"
     CDS             complement(429..1850)
                     /gene="nhaA"
                     /locus_tag="VFQ61_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET9957836.1"
                     /translation="MATTSSPISVPLSSAPPEAWEPLLRVARLAGKPLERFLRIEAAS
                     GILLLAAAASALVWANSPWAESYHQLWRTPLSVGVGQLRFERTLEWFVNDILMVIFFF
                     VVGMEIRREIHHGELSEWRRATLPVAAALGGMLVPAGVYLGLAGERLTRPGWGVSMAT
                     DIAFAVGILTLLGKRVPAALRVLLLALAVIDDLGAIVVIALFYSSGVSLSGLLVAAMG
                     LAGVFVMQRLGLRSKIVYVVPAFVAWAGIYGAGIHPTIAGVIVGLVTPVRAWLGPEGF
                     LVSARRELDVLAQVPAGVLSSHELAGSLRKVELARREAMSPAESLIATLHPWVAFGIM
                     PVFALANAGVSLTGGPLDDAARRIVFGVAIGLAVGKPAGVLLVSWIALKFKLATLPRG
                     LTFAHLLVLGVVAGIGFTMALFVAQLAFTDQRLLAAAKVGVLGASGLAAVAALALGRL
                     LLKPAEGASAALTADEAERSTEG"
     gene            complement(2143..>3668)
                     /locus_tag="VFQ61_25240"
     CDS             complement(2143..>3668)
                     /locus_tag="VFQ61_25240"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET9957837.1"
                     /translation="FLATYPELLQGRLAGPVSVGVLLIAAVAPWSLRSESSELQLIAR
                     GRVLPAVLAVLQRATDLDGDGASAWFGGRDCAPFDRSRNPGAQEIPGNHIDEDCDGKD
                     EGTSATRQLAPTYSFWKAARNPPNVIWYVVDSLRFDHLKTYGYPFETAPALSEFAAES
                     LVFESAFSQSSTTALSIPSMLSGRNPEQMLFDAGGFPRARPGQFYVQSVFGRNGFLTG
                     LIINDWTRQNLPGIQHEFQRVLSAPPEHNWRSADHAVLQLIDFITVAKSERRPFFAVL
                     HVDDVHHPYLSAQGKAVQSFPAPGEVGRYDSGIAVFDQSFRIAIEYLKRSGLWDDTVI
                     IVTADHGEEFGEHGGTVHSRTCYSEVTHVPLVMRVPRVSARRIKSTVALIDIVPTLLE
                     LLDRREEGGALEGQSLLIPALEPSELGSSRPVFCTIFQIADPQRPFFTRAVRKSQWSL
                     FEESRSGALELYDVKSDPGERINLAMEVGLQPILRELRASLARPRSGNLYQITQAAR"
BASE COUNT          836 a         1166 c         1072 g          594 t
ORIGIN      
        1 gaagatcacg agcgctcccg actcgactcg tgagatgacc ccgtcaagca gcaacaaggg
       61 tagcagcgcg gtgctgagcg cgagcactgg tagctctcgc ttccgaagcg cgccgtccac
      121 ccgagccgga cgaacgagta ccgcaagtcc caagatcagg ccgatattgg ccacgttcga
      181 accgatcaca ttgccgagcg cgacctcgcc gtgtccggtg cccgcagcct cgatgccaac
      241 gatgatctcc ggcgccgagg taccgtacgc gacgacggtc agtcctacca ggatctgggg
      301 cacgcgcagc gcgagcgcga gcgcggaggc tcccccgacg aaccattccg cgccgaagta
      361 gagtagaagg ccgccgccga gtagctgaat ccattgaatc accaagattg cggaagtgta
      421 tccaacctct atccttcagt cgatctctcc gcttcatcgg ccgttagcgc ggcactagcg
      481 ccttcggctg gcttgagaag aaggcgccca agcgcgagcg cagcaaccgc cgccagtccg
      541 ctcgcaccaa gcacacccac cttcgccgcg gctaacagtc gctggtcggt aaacgcgagt
      601 tgcgcgacaa acagcgccat cgtaaatccg atcccagcta caactccgag aaccaggaga
      661 tgtgcgaagg tcaaaccgcg cgggagggtg gcgagtttaa acttcaatgc gatccaactc
      721 acgagcagaa cacccgctgg cttccctacc gcaagaccga tcgcgacgcc gaacacgata
      781 cgtcgcgcag catcatcgag cggccctccc gtcaggctga ccccagcatt cgccaacgca
      841 aatactggca tgatcccgaa agcaacccac gggtgcagtg tcgcgatcag gctctccgcc
      901 ggggacatcg cctcacgccg agcaagctcg acctttcgca gtgatccggc caattcgtgc
      961 gacgagagca ctccggcagg tacctgcgca agcacgtcca gctctctgcg cgcgctgacc
     1021 aaaaacccct cgggcccaag ccaagcccgc accggtgtaa ccagcccgac tatgacgccg
     1081 gcgatagtgg gatggatccc tgctccgtaa atgcccgccc acgcgacgaa cgccggaact
     1141 acgtagacga tcttggagcg cagccctagg cgctgcatca cgaacactcc agctaaaccc
     1201 atggccgcta cgagcaggcc gctgagcgat actccggagg agtaaaagag ggcaatgacg
     1261 acgatcgcgc cgagatcgtc gatcaccgcc agggcgagga gtaacacgcg cagcgccgcc
     1321 gggacgcgct tgccgagcaa cgtgagaatg cctacggcga acgcgatgtc tgtcgccata
     1381 gacacgcccc agcccgggcg agtcaagcgc tccccggcca gccctaggta cactcccgcg
     1441 ggcacgagca tgccacccaa cgcagcggcg accggcaggg tcgcccgtcg ccattctgac
     1501 aattcgccat ggtggatctc gcgtcggatc tccataccga cgacgaagaa gaagatcacc
     1561 atcaagatat cattcacgaa ccactcgaga gttcgctcga acctgagctg cccaaccccg
     1621 acactcagcg gggtacgcca aagttgatga tagctctcgg cccacggcga attcgcccat
     1681 acgagcgccg aagccgccgc ggcaagcagg agaatgccgc tcgccgcctc gattcgaagg
     1741 aatcgctcca aaggcttgcc ggcgagccgc gcaactcgca ggagaggttc ccacgcctcg
     1801 ggcggtgcgg aagaaagggg gactgatatg ggggacgaag tcgttgccat gcgcatctca
     1861 cgccggcgta aagaaccagc gtccacgtca cggcattcct tcgagggttc gagaagaatg
     1921 ggcgtccagc ccggaatcct aggcttcggt ctaacaagca ccgtaagggg aagagggtta
     1981 cggccgggct gaacgctcga gcggagacta tcagtttcgc ccgtctaccc caagtgcgat
     2041 ttcatgtcct accgcgagac acttcgggaa agacctggca acagaagaac accaactcgg
     2101 gagagattgg gtcagttcga cacagccacg caccgcgacg attcaccgtg ccgcttgcgt
     2161 gatttgatag aggttcccgg acctgggtct agccaagctc gcccgcagtt cgcgcaaaat
     2221 gggctgaagt ccgacttcca tggcgagatt gatgcgctct cccggatcag acttcacatc
     2281 atacaattcg agtgcacccg acctcgattc ttcgaacaaa gaccattggg actttcgcac
     2341 cgctctcgta aagaacgggc gttgtggatc ggcaatctga aagatagtgc agaagactgg
     2401 ccggctcgag cccaactccg acggttccaa ggctgggatg agcaaactct gcccttctag
     2461 agcgccgccc tcctcacgcc ggtccaacaa ctccaggaga gtcggaacga tatcgatcaa
     2521 agccaccgtc gatttgattc ggcgcgcgct gacccgagga accctcatga ccaacggcac
     2581 gtgggtgacc tcagagtagc aagtccgaga atgcacggtg ccaccgtgtt ctccgaactc
     2641 ttcaccgtga tcggcagtga cgattataac ggtgtcgtcc cagaggccag aacgctttag
     2701 gtactcgatg gcaattcgaa aggactggtc aaagactgca atcccagagt cataccgccc
     2761 aacttcaccc ggcgcgggga aggactggac ggctttgcct tgcgcagaaa ggtacggatg
     2821 gtggacatcg tcgacatgca gcactgcgaa gaatggtcga cgttcgctct tcgcgaccgt
     2881 gatgaagtca atcaattgca acaccgcgtg gtccgcactt ctccaattgt gctctggtgg
     2941 tgcgctcagc actctttgga actcgtgttg aatgcccgga agattctggc gcgtccaatc
     3001 attgatgatg aggccagtca gaaatccgtt acgcccaaag acgctctgca cgtagaattg
     3061 cccgggccgg gctcggggga atccccccgc gtcgaagagc atttgctccg gattgcggcc
     3121 ggatagcata ctgggaatgc tcagcgcggt cgtagatgat tggctaaatg cggactcgaa
     3181 cacgagagac tccgcagcga actcggatag agccggagcg gtctcgaaag ggtacccgta
     3241 agtcttgagg tggtcgaagc gcagcgagtc cacaacgtac cagatcacgt tcggtgggtt
     3301 gcgagcggcc ttccagaagg agtacgtggg ggcgagctga cgagttgccg acgtaccttc
     3361 gtctttgccg tcgcagtctt cgtcgatgtg gttccctggt atctcctgcg cacccggatt
     3421 gcgcgagcga tcgaagggcg cacaatcgcg cccgccgaac caagccgacg caccatcccc
     3481 atcgagatcg gtggcgcgtt gaagcacggc cagaactgca gggagaacgc gtccacgagc
     3541 gatcagctgc agctcggaag actcggagcg caaggaccaa ggcgctaccg cggcgatcag
     3601 caacacccca accgagacag gccccgccag ccgtccctgg aggagctcag ggtaggtagc
     3661 caggaaca
//
LOCUS       DASTKU010000722         6874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_584806, whole genome shotgun sequence.
ACCESSION   DASTKU010000722 DASTKU010000000
VERSION     DASTKU010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584806"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1938)
                     /locus_tag="VFQ61_25245"
     CDS             complement(<1..1938)
                     /locus_tag="VFQ61_25245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957838.1"
                     /translation="MAHLIRALGWIRFLPFFLIGWVAFVSSCSLPDYQFRTGNCDNGV
                     ADGNETDVDCGGSCAKCTLGKYCLTSADCANESCEDKHCQPKSCTNHRRDLSGPMGSF
                     ETDVDCGGSVCPACRANQRCQIDSDCSSGSCNGGRCSPESCTDGEKNDKETGTDCGGE
                     ECATCKNGETCDRNEDCVSGNCSSGTCSGGDCVDHIKNGNETGVDCGGSCPEDCGYLE
                     ACKTNQDCENRTCVAGHCQDETCANGKLDPSETDIDCGGGKCVGCDSGEVCEVAPDCA
                     SGICIEGRCQAPRCDDGSRNGEETDVDCGGPVCPDCQDDARCSFALDCVSRVCKGGFC
                     LAANCSDETLNGAETSVDCGGVSCSACVKAQGCRIGADCETGLCVSDICTTERCQNGS
                     LDGMETDEDCGGDECPKCAPGGSCQGDTDCLSRRCVDGVCAPPACDDGVRNGGETGVD
                     CGGGTCPARCEYGSSCGGGSDCASGSCIDQVCAPPRCDDRVANGTETDVDCGGRQCGP
                     CGPEASCESNRDCQSGLCSQDKCLGADCSDGILNGQESDVDCGGQARGSKGELKCPRC
                     DLREACQENDDCLSSFCSAALLCVDPSCGNGQRDASESDVDCGGSCGATCEEGAACGR
                     DDDCNSFRCSAGRCAVPTCQDR"
     gene            2134..3285
                     /locus_tag="VFQ61_25250"
     CDS             2134..3285
                     /locus_tag="VFQ61_25250"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9957839.1"
                     /translation="MADASPPAAQTEILARLESLIGEDAARLAELSWSALLLSEGISG
                     GVSERTPDEASDGSSERSSGKYPGGSPEGELELAPSAATLEHLLLHRRLLADWLARSE
                     ASELSRGVESTQPALAGRPSFAAQPDRYLAERCARWLSYKNQFAFGADTQPELFRLFA
                     DGRTKLASALSAPADLVEARVRAAWAEVRGALSRWAKQRAGAHPRAVVCSEYSASLQL
                     EVLGLDLESLAEPILDVGCGERAELVTLLKARGKRVVGLDRYAQKEGCLVADWLAFPY
                     ESARWGTVLSHQAFTLHFAHQHLANAELSRSYAATYMRILRSLAPRGRFAYAPSLPFL
                     EELLPPSEFSVTRRSILAATSPAKPAAIEELERKSGVALQSSTLVQRSP"
     gene            complement(3297..3722)
                     /locus_tag="VFQ61_25255"
     CDS             complement(3297..3722)
                     /locus_tag="VFQ61_25255"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9957840.1"
                     /translation="MRFLHTMLRVRDLKAALDFFVGALGMIELRRHENSQGRFTLVFL
                     GTGAPGDAAEIELTFNWDQTEPYPTGRFFGHLAFEVDDIYATCESLERNGVSVLRPPR
                     DGRMAFVKSPDGQSVELLQRGAALPPKEPWLSRENRGSW"
     gene            3795..>6874
                     /locus_tag="VFQ61_25260"
     CDS             3795..>6874
                     /locus_tag="VFQ61_25260"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9957841.1"
                     /translation="MRLFSSVRWSLLLGLSVACGGNPDVVTPTETGNSTGGRSSGGGG
                     SGNSGGRTVIPVGGDSDPGEGGTTGTPLDPCLEQECGSGQRCEMNASGEAECVDVSCK
                     ELKCGKKEECQPAPGGGHRCVDIGCDSDADCPEERFCNADGVCADDACSAESRRCDGN
                     ELLVCGSNGGVEESPYACSSLGKDRFQSECSEAIASAIGCTCEDDWDCPPNTVCEVSQ
                     CTGTGVAPTCTLPPVPFENVLPTREFRWGGSAYATPEAPGKAFPWSAQVASTPIIANL
                     DDDNGDGKVNELDFPEILFLTYHRYPGTTATSAPQPLTEGIVRAIHGGGEHKGEDFFA
                     LCGNPARPAQPRSSYSDAKGAYWSEGAAPLNDCDSDTTNNGTNSPLEQNSRKAALGRP
                     GGALAVGDLDADGLPEIVVPTENYGIQILSNRGEVLLTSAANLFKPYSSNDDWHYPSP
                     AIANLDFKGLAEIVVGNYVLTLKKDGANFLIDQVRGGAVGTGAMVFNDDNQDFGPMTC
                     VADVRPDLPGLEVVAGPTLYRFDPNQAGLVEVWTAKTGLTYPDGFCAVADVLGKDPEA
                     APGPDNPLDRVPEVIVVADGHLFVLSAQDGSVLRDLALGGGRQGGAPNVDDFDGDGFP
                     EIATALSDFYTVVDLQEPDAAHCPEWPSLLRADEAPPGRNPERDPGADCKSDKDCNSG
                     AVCNAVTHACVCLHNSWRRDTEDDSSKSTSSSVFDFNGDGAAEVVYNDECYFRVYDGA
                     TGGEYLAIPSLSRTIVENPVVADVDNDGNAEIVFVQNNDLIQCNETPLDSFPNGNNDT
                     DPQSLPNGIEVWGDPSDVWVAARRIWNQQSYHVTNVLEGGGIPLHEPESWKPLHDRLY
                     NTYRSQPRVYGVAPDLALVALQVSSPDTECGKLSDNMQVTVLVKNEGDLRVGPGVVLD
                     FYGTWGGKETTLRDDLGEPVQVTLDKSLEPSASTLVTIQYSVGNNPAPNDKALPDRLR
                     VAIDGGNGAKGDGAERECHEDNNEIEGPVEAGEALADLAVEVSAGDCGGDVEIKV"
BASE COUNT         1275 a         2209 c         2150 g         1240 t
ORIGIN      
        1 ccggtcctga caggtaggga ccgcacaacg tcccgcgctg caccggaagc tgttgcaatc
       61 gtcgtcccgg ccgcacgcag ccccttcttc gcaggttgct ccacagctgc cgccgcagtc
      121 cacgtccgac tccgaggcgt cacgctgacc attcccacac gacggatcga cgcagagcag
      181 cgccgccgaa caaaagctgc tgaggcagtc gtcgttctcc tgacaggctt cgcgcaaatc
      241 gcaacgaggg cacttcagtt cgcctttgga gcctcgggct tggccgccgc aatccacgtc
      301 gctttcctgc ccgttcagaa tgccgtcgct gcagtccgct cccaggcact tgtcttgaga
      361 gcacaagccg ctctgacaat cgcggttact ctcacagctt gcttcggggc cgcacggtcc
      421 acattgccgg ccgccgcagt cgacgtcggt ttcggtgccg ttggcgacgc gatcatcgca
      481 acgtggcgga gcacacacct ggtcgatgca agagccgctg gcacaatccg aacctccgcc
      541 acaactcgat ccatactcgc agcgcgcggg gcaggtgcca ccgccgcagt ccacgccagt
      601 ctcgccgccg ttgcgcacac cgtcgtcaca agccggcggg gcgcaaacgc cgtcgacaca
      661 acggcggctg aggcaatcgg tgtcgccttg gcagctcccg cctggcgcgc acttcggaca
      721 ctcgtcaccg ccgcagtcct catcggtctc catgccgtcc agcgagccat tttggcaacg
      781 ctcggtcgtg cagatgtccg atacacagag gcccgtctcg cagtcggcac caatgcggca
      841 gccctgggcc ttgacgcagg ccgagcagct tacaccgcca cagtcgacgc tggtctcggc
      901 gccgttcaac gtctcgtccg agcaattcgc cgccagacag aaccctccct tacaaacccg
      961 actcacgcaa tcgagcgcaa acgagcagcg tgcgtcgtcc tggcagtccg ggcacacggg
     1021 gccgccgcag tcgacgtccg tttcctcacc gttccgcgat ccgtcgtcgc aacgcggcgc
     1081 ctgacaacgg ccctcgatgc agatgccgct cgcgcaatcc ggggcgacct cacagacctc
     1141 gccactatcg cagcccacgc acttgccgcc cccgcaatcg atatcggtct cgctcgggtc
     1201 gagcttaccg ttggcgcaag tctcgtcttg gcaatgcccc gcgacacaag tgcggttctc
     1261 gcagtcctga ttggtcttgc acgcttcgag gtaaccgcag tcttcggggc aggatccgcc
     1321 gcagtccacc cccgtttcat tgccgttctt gatgtgatcg acgcaatccc cgccgctaca
     1381 cgtaccgctc gaacaattgc cgctcacaca gtcttcgttg cgatcgcagg tctcgccgtt
     1441 tttgcaggtc gcgcactcct cgccgccgca atcggtcccc gtttctttat cgttcttctc
     1501 gccgtcggtg cagctctcgg gtgagcatcg cccgccgttg cagctaccgc tgctacaatc
     1561 cgaatcgatt tggcagcgct gattggcgcg gcaggcggga cacactgacc cgccacaatc
     1621 cacgtccgtt tcgaatgacc ccatgggtcc gctcaggtct cgacggtgat tggtacagct
     1681 cttgggctgg cagtgtttgt cctcacacga ttcgttcgcg cagtccgccg aggtgagaca
     1741 gtacttgccg agcgtgcatt tcgcgcagct tcctccgcaa tcgacgtcgg tttcgttgcc
     1801 atcggccacg ccgttgtcgc agttgccagt ccgaaattgg taatccggca gcgaacaaga
     1861 ggaaacgaaa gcgacccagc cgatcaggaa aaaaggaagg aacctgatcc atccaagggc
     1921 tcggatgagg tgtgccaatt gcgagagctt agcttgaatt cgttacggtc gatgctgctc
     1981 cttttgcgaa ctcggcttcg gcattcgcga acccggcaga cgcgccaagg ctgcagcact
     2041 tcccgagccc acccgagcat ccgatccctc cgaccgcgag gctcatccac cgagtggctc
     2101 acccgtgggg acgcgtgctg ctatacagcg cgcatggcag atgcctcccc tcccgcggcg
     2161 cagacggaaa ttttggctcg gctcgagagc ttgattggcg aagacgctgc gcgcttggcg
     2221 gaactttcgt ggtctgcgct gttgctttcg gagggaattt cggggggagt ttcagagcga
     2281 acgccggacg aagcttcaga cggctcctct gagcgctcct ctgggaagta cccgggaggg
     2341 tcgccagaag gcgagctcga gctcgcgccg agtgcggcca ctttggagca tcttttgcta
     2401 caccgccgct tgctagcaga ttggttagct cggtcggagg catccgagct ctctcgcggg
     2461 gtcgagtcta ctcagccagc cctcgcgggc cgccccagtt tcgcggcgca gccagaccgc
     2521 tacctggcag agcgctgtgc ccgctggctg agctacaaga atcaattcgc cttcggcgcc
     2581 gacactcagc cagagttgtt tcggctgttc gccgacggcc gaacgaaatt ggcctctgct
     2641 ctgagcgccc cggccgatct ggtcgaggcg cgagtgcgcg cggcttgggc ggaggtcaga
     2701 ggggcactga gtcgctgggc gaagcaacgc gcgggagcgc acccgagagc ggtggtctgc
     2761 agcgagtatt ctgcctcact tcagctcgag gtcttgggtc tcgacctcga gtccctggca
     2821 gagccgatcc tggatgtcgg ttgcggagaa cgcgccgagc tggtgacgct gctcaaggcc
     2881 cgcggcaagc gtgtcgttgg cttggatcgt tacgcccaga aggagggctg tttggtggcg
     2941 gattggctcg cgttccctta tgaatcggcc cgctggggaa cggtgctctc gcatcaggcg
     3001 ttcacgctgc acttcgcgca ccagcacttg gcgaacgctg agctatcgcg cagctacgcg
     3061 gcaacgtata tgcggatcct tcggtccctc gcgccccggg gccgttttgc atacgcgcca
     3121 agcctaccgt ttttggagga gctgctgccg ccttctgagt tcagcgtaac tcgccgctcg
     3181 atcctcgccg caacctcccc cgccaaaccg gctgcgatcg aggagctcga gcgaaagagc
     3241 ggcgtagcgc tgcagtcgtc gacgctggtc cagcgctcac cgtgaccggc ggtcggtcac
     3301 caggagccgc gattttcacg cgatagccag ggttccttgg gcggcaaggc tgccccgcgc
     3361 tggagcaact ccacggactg cccgtcgggg gacttcacga acgccatccg gccatcccga
     3421 ggcgggcgca gaacactgac gccgttgcgc tccaaagatt cgcaagtcgc gtagatatcg
     3481 tcgacttcga acgcgaggtg gccgaagaag cgccccgtgg ggtacggctc ggtctggtcc
     3541 caattgaacg tgagctcgat ttccgcggcg tcccccgggg cgccggtgcc caggaaaacg
     3601 agggtgaaac ggccctggct gttctcgtgg cgacgcagtt cgatcattcc cagcgccccc
     3661 acgaagaaat ccagggcagc cttcaagtct cgaacgcgca gcatggtgtg gaggaatcgc
     3721 atgacggtga atggttagcg caaaaattcc ggactgcgct agagccgcat ttctgagcta
     3781 cattcgacct cctcatgagg ctcttcagca gcgtgcgctg gtcacttcta ctgggtcttt
     3841 ccgtagcgtg cggaggtaat ccggatgtcg tgactccgac cgagaccggc aattctacgg
     3901 gcggaagaag ctccggcggt ggaggctccg gcaacagcgg tggacgcacc gtcatccctg
     3961 tgggcgggga ctccgacccc ggagaaggag gcacgactgg cacgcccttg gatccctgcc
     4021 tcgagcagga atgtgggtct ggccaacgct gcgagatgaa cgcgtcgggc gaggctgaat
     4081 gcgtggacgt cagctgcaaa gagctgaagt gcggcaagaa ggaggagtgc caaccggcgc
     4141 cgggtggagg gcatcgctgc gtcgatatcg gttgcgattc cgacgccgac tgcccggagg
     4201 aacgcttctg caacgcggat ggggtatgtg cggatgatgc ctgttcggca gaatcccggc
     4261 gctgcgatgg caacgagctg ctcgtttgcg ggtcgaacgg cggcgtcgag gagtcgcctt
     4321 acgcctgcag cagtttgggc aaagatcgat ttcagagcga atgctccgaa gcaatcgcga
     4381 gcgcgatcgg gtgcacgtgt gaggacgact gggactgccc gcccaacacc gtttgcgagg
     4441 tctcccaatg cacgggcact ggcgtcgctc ccacctgcac gctgcctccg gttccgttcg
     4501 aaaacgtgct accgacccga gaatttcgct ggggagggag cgcctacgcc acccccgagg
     4561 ctccgggcaa ggcgtttcct tggtccgctc aggtagcgtc cactccgatc atcgccaacc
     4621 tggacgacga caatggtgac ggcaaggtca acgaactcga ctttccggag atcctcttcc
     4681 tcacctatca ccgctacccg ggtactacgg ccacgagcgc gccccagccg ctcacggagg
     4741 ggatcgtgcg cgcgatccat ggcggcggcg agcacaaggg cgaggacttc ttcgcgctgt
     4801 gcggcaaccc ggcccggccg gctcaaccgc gcagttcgta ttcggatgcc aagggcgcgt
     4861 actggagtga aggcgccgcg cccctcaacg actgtgactc ggataccacg aacaacggga
     4921 cgaacagccc gctcgagcag aacagccgca aggcggcgct cgggcgtccc ggcggcgcgc
     4981 tcgccgtagg agatctggat gccgatggtc tcccggaaat cgtagtcccg actgaaaact
     5041 acgggattca gatcctctcg aatcggggag aggttttgct gaccagcgca gccaacttgt
     5101 tcaaaccgta ctcgagcaac gatgattggc attacccatc gccggccata gccaatctgg
     5161 atttcaaggg cctcgccgaa atcgtggtcg gcaactacgt cctcaccttg aagaaggacg
     5221 gagccaattt cttgatcgat caagtgcgag gcggcgcggt gggcacgggc gccatggtct
     5281 tcaacgacga taatcaggat ttcgggccca tgacgtgcgt cgccgacgtg cgaccggatc
     5341 tgccgggtct cgaggtcgtc gcggggccaa ccttgtatcg tttcgacccg aatcaagctg
     5401 ggctcgtgga agtctggacg gccaagacgg gtctcacgta cccggatgga ttttgtgcgg
     5461 tggcggacgt acttgggaaa gatccagagg ccgcaccggg cccggacaac ccgctcgacc
     5521 gagtgccgga ggtgatcgtc gtcgccgatg gacacctctt cgtactgtcc gcccaagacg
     5581 gcagcgtgct ccgagatctc gcgctgggcg gcgggcggca agggggcgcc ccgaacgtgg
     5641 acgacttcga cggggatggt tttccggaaa ttgcgaccgc cctctccgat ttctatacgg
     5701 tcgtggatct gcaggagcca gatgcggccc actgccccga atggccaagc ctactgcgcg
     5761 cggacgaggc tccccccggg cgaaacccag aacgggatcc aggcgcagat tgcaagagtg
     5821 acaaggactg caactcgggt gcagtgtgca acgccgttac tcacgcgtgc gtgtgtttgc
     5881 acaacagttg gaggcgagac accgaggatg actcgagcaa gtccacgtca tcgagtgtct
     5941 tcgatttcaa cggcgatggc gcggcagagg tcgtgtacaa cgacgagtgt tatttccgag
     6001 tttacgacgg cgctacgggc ggcgagtact tggccatccc atctttgagt cggacgatcg
     6061 tcgagaaccc ggtggtggcc gacgtggaca acgacggcaa cgccgagatc gtgttcgtgc
     6121 agaacaacga cctgattcaa tgcaatgaaa ccccgctcga tagctttccg aacggcaaca
     6181 acgacacgga tccgcaatcg cttcccaacg gcatcgaagt gtggggcgac cccagtgacg
     6241 tgtgggtcgc ggcacgccgg atctggaacc agcagagcta tcacgtaacg aacgtgctcg
     6301 aaggcggcgg aatcccgctg cacgagcccg agagctggaa gccgctgcac gatcggctct
     6361 acaacaccta tcgctcacag ccccgggtct acggggtggc gcccgatctg gcgttggtcg
     6421 cgcttcaagt ctcctcgcca gacaccgagt gcggcaagct ctccgacaac atgcaggtca
     6481 ccgtgctcgt aaagaacgag ggcgacctgc gcgttggccc cggtgtggtc ttggacttct
     6541 atggaacctg gggtggcaag gaaaccacgc tgcgcgacga tctgggcgaa cccgtgcagg
     6601 tgacgctgga caagagcctc gagccctccg cttcgacttt ggtcaccatc cagtacagcg
     6661 tcggcaataa cccggccccc aacgacaaag ccctgcctga ccgtctgcgt gtcgcgattg
     6721 acggcggcaa tggcgcgaag ggtgacggcg ccgaacgcga gtgccacgaa gacaacaacg
     6781 agatcgaagg gcccgtcgaa gccggagagg cactcgcgga tctcgcggtc gaagtgagcg
     6841 ctggcgattg cgggggagat gtcgagatca aagt
//
LOCUS       DASTKU010000723         6163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1925852, whole genome shotgun sequence.
ACCESSION   DASTKU010000723 DASTKU010000000
VERSION     DASTKU010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925852"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFQ61_25265"
     CDS             complement(<1..878)
                     /locus_tag="VFQ61_25265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957842.1"
                     /translation="MINCPFVTPKTDGSEGNFPALEQLWQVTANSVGSAFFAAVCVAL
                     SRLLPNTEVLYATASDRVARLEPGPESADVASAANEGSVEPSLPVEIRSRASGGKAQH
                     QTSRREVSTLLERVLSLGHVLLPKAARDVFPDDPLLEASNAQGCIAVRAADSLDGDGA
                     VLLMSQTPLSSEVALVWLNILKSEISRERSQRATRWLRVTAELSAHLTSQSPPASKLE
                     KLCQVLQAELGVPGVGVYAPKSRFVRSPVWIARGALSESLSRSIPADPVPEEMAWLAT
                     EPDTVGVQTPVLCLRVG"
     gene            2687..3346
                     /locus_tag="VFQ61_25270"
     CDS             2687..3346
                     /locus_tag="VFQ61_25270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9957843.1"
                     /translation="MSFVEFPKSAHRSAHHSLGPTDKAHPAPGPVKALDSPAGTKLGD
                     CERAHFNARVLELYVPLLSAARRLTSCEEDALDLVQDTFERALRNFGKFSGDNLQGWL
                     FTLMKHLFFDERRRDRVFVRTKEELRVSSVGTLEPEQARLQASCEYLERALASIDGVS
                     REILLLRELEALTYQEIAGRLGISASSAGVRLHRARVRLQQALEAGRLEAPRAEPPPS
                     E"
     gene            3797..4366
                     /locus_tag="VFQ61_25275"
     CDS             3797..4366
                     /locus_tag="VFQ61_25275"
                     /inference="COORDINATES: protein motif:HMM:NF015065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957844.1"
                     /translation="MAALNVITDETSTQDFLVIKDFLASQGIDFGRFQRNPAVLSLAE
                     PHTLTDEQRAELIALYPDVREKFERRTGFRCDVVCFYPELPQLNFIIDKFGSIHYHFE
                     TEYWYFVDGEAKFGFLGTNGTKFELTVQGGEFLQVPEGCWQWFQLTPIKRMKSIRYFY
                     STESQKPRVPYKFGGSDAQAGSKPEVVGS"
     gene            4363..5178
                     /locus_tag="VFQ61_25280"
     CDS             4363..5178
                     /locus_tag="VFQ61_25280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9957845.1"
                     /translation="MTLSSASVTMLAACAARASFADRLQDSFSNEILRHLPDADAKRD
                     TLDQASRGMFSRSILLRTLYFRERLRATLRPGSQLVLLSHGLDCELMKSGEHALFAVD
                     HPASQRFTRAALEAAKIDVRVQFIAHDFARDTPSQLWSSLRAAGFSADAHSVVLWEGA
                     TYYLPETAVVPVLSSFASRCARVDLFADFLNHGGYFVNGRVVSPGVGQSLAFVESLGE
                     PWVGFFQTDTLSRTLQDAGFQSVNCEDRSGVEQRILGERLMQPNMMFFMEAHK"
     gene            5175..5813
                     /locus_tag="VFQ61_25285"
     CDS             5175..5813
                     /locus_tag="VFQ61_25285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957846.1"
                     /translation="MTETALSGRAFVASLLQDVDETVQRNKGVMPPGMDGPVLPEPIL
                     RHWLTFAVYYEKAATSFIGGWMRSTQEPDAVVQFAHQIEDEANHFIWLRKHLLSFGID
                     AATFKPPKEWALLMEEFYPGLDHLVERLAAHNIASETGALGFMEYNMDKFPAEIQATV
                     RKVARDEKFHVAIGKRLLSKYCTTAELQERARGATRSALDLMSAARRVFVRV"
     gene            5835..>6163
                     /locus_tag="VFQ61_25290"
     CDS             5835..>6163
                     /locus_tag="VFQ61_25290"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET9957847.1"
                     /translation="MRRTVITGIGVIAPNGRSPREFWDNANRGRSFIEADPEMEQLGI
                     KSRVTCRIREFDPQDHMDSELARAVAGEDRFVQIGVAAGAGALRDSACLDHHPGTRVG
                     AIYSSAIG"
BASE COUNT         1206 a         1795 c         1941 g         1221 t
ORIGIN      
        1 cccacgcgaa ggcaaagcac cggcgtctgg acccccaccg tgtccggttc agtcgcgagc
       61 caggccatct cctcgggcac cggatcggcc ggtatactcc gggataaaga ctcgctcaga
      121 gcgccgcgcg cgatccacac cggggagcgc acgaatctgc tcttcggggc gtagacgccg
      181 accccgggca cgccaagctc tgcctgcaag acttggcata gcttctcgag cttcgaagcc
      241 gggggcgatt gggaggtcag atgagcggag agctctgccg tcaccctcaa ccacctggtc
      301 gcccgctggg aacgctcccg ggatatctct gatttgagga tattgagcca aaccagcgcg
      361 acctcgctgc tcaagggcgt ctggctcatc aacaagactg cgccatcgcc gtcgagcgag
      421 tcggcggctc gtaccgcaat gcagccctgc gcattggaag cctcgagaag tggatcgtcc
      481 gggaaaacat cgcgagccgc cttcggaagc agcacgtggc ccaagctgag cacgcgctcg
      541 agcaaggtgc tcacttcacg ccgggaggtc tgatgttgag ctttgccccc cgaggcgcgc
      601 gagcggatct cgacggggag agacggttca acagagccct cattcgccgc agaagcaacg
      661 tctgcggact cgggccccgg ctcgagtcga gctacgcgat cactggctgt tgcataaaga
      721 acttctgtat tcggaagcag acgactcagg gcgacgcaca ctgccgcaaa aaacgctgat
      781 ccgaccgaat ttgcggtcac ctgccagagc tgttcgagag cggggaagtt gccctccgac
      841 ccatccgtct tcggggtcac gaaaggacag ttgatcaccg ccaccccgaa cgcaatgaga
      901 acggctgacg atcgtcgcga gatgtccggt cgccgggcca gatgtggtca gcggtgttcg
      961 ctgcggctga gagccatatc gctcgcttct acgtggcacg cggttgggca gggtcgtgga
     1021 gctagagcca tctcctcctt gggcggagcg tcatctgatg gagccgcgga gcctcatgtg
     1081 gcggcgcctc acatgccggc gtcaagctcg caagagactc ggtggtcgaa gttccaccga
     1141 agccctcatg aggcggacgc gcccggtgcg cagagtggga gtcgcggagc atcatagtgg
     1201 tgaccgcgaa cagagcgctc cagagctttc gcaggcggcc ggtggcgaac tgaggagcca
     1261 taaggcaatc gcgccgagcg catgcgtaga ttgccgagtc actcgccagg ataccgtagt
     1321 gcggactcgg tgtcagacgc ggatgccacc gctcgagtcg agtggactca atgtgtaata
     1381 taaaacattt tcagaaacat ctccggcttc aaaaatattg aatcggcgcg aatcgccccg
     1441 tattcgcggt ctggaattgc gtcccgctct ctcgaagatg caagtcgtat ccgatgcgaa
     1501 tcgatcgcat ccaacggagt tgggacagga gagaccaaac ccgagagtcc tccatcaagt
     1561 cgactcgatc tcgacgcgtt gttcttgctc tgagcggatc gctgtgcgat cttgcgcgcc
     1621 gtacgctggt ggtcgaggga gcaagtgtgg aagctgttgt tttgcggtat ctgcagtctg
     1681 atttctcgag acttgggtct tgggactggg aaaaattgcc atggctcggc agcggtatcg
     1741 tgcggggtgc cgttggtgcc tttggcctag atcgaggcag tcgccgcgat ccggaactag
     1801 aggtgaacaa ggcgtactca ccacgtggtc ggagccgaag ctagccactt ggcccatgac
     1861 tgcagcggaa cgatgcatcg aaggacacga gcgcactatt cttgagcgta gctcggcaga
     1921 cactgcgcag attacgtgtc attttcgcgc tcgtccgctg caatcggtgg ggaagcccac
     1981 tccatagcgt tgacccgacc acctctcctt cgctctgcaa accagtcgct ctgcaaacca
     2041 gtcgctctgc aaactagtcg ctctgcaagc tagtcgctct gcaaaccggg ttttggcaga
     2101 gtgataaacg accaagctca gcaagtgagc cgagtcgaat ccgaggggca aatgctcttg
     2161 cgtgcggctc tgtgaactgc atttccgcgg gaaactaggc gttgcagagc ttctgttgac
     2221 ttgtatttga tcatgtcgat tgatgggctc gcggcgctcg ggcatccact gcacctcgga
     2281 gatatgccgg ttcatctgcc cgaccggaag gatcgacatt caatgggatg gcggcggaag
     2341 actgaacagg acgcacgctc gcagcgaatg agttggtacg accgagcgaa caggcaccca
     2401 atattcggtg cagggcatat gtaattgggc tcgagacgag accttgggaa tcgaggatcg
     2461 tagtaatgag gttcttctta cgaacacgct ttttcggttc tctccttcat gataggaatg
     2521 cgcgcccaac tgagtggcag gcggaataga agcgctcact gagcgattat gagggtcttc
     2581 gccaggctat ccgcctcccc gctccgccct gatttgcgaa agatggtcgg gatcaggacg
     2641 gtcgggagag aaactctgag actgcaagcg tgcccgagaa ttcgccatgt cattcgtaga
     2701 attcccgaaa tctgcccatc gatccgcgca tcactctctg ggtcctacag acaaggcgca
     2761 cccggcgccg ggccccgtca aagcgctcga ttcgccagcg ggcaccaagc tcggagactg
     2821 cgagcgtgct cacttcaacg cgcgggtcct cgaactttac gtgccgttac tcagtgccgc
     2881 ccgccgcctg acgagctgcg aagaggatgc gctagacctc gttcaggaca ccttcgagcg
     2941 cgcgctgcgg aactttggaa aattcagcgg agacaatctc cagggctggc tgttcaccct
     3001 catgaagcac ctgttcttcg atgagcgccg gcgcgatcga gtattcgtgc gcacgaagga
     3061 agaactgcgt gtcagttcgg ttggtacgtt ggagcccgag caggcgcgct tgcaagcttc
     3121 ctgtgagtac ttggaacgcg cgctcgccag catcgacggc gtgtctcggg agatcctgct
     3181 cctgcgcgag ctcgaagcgc tcacctacca agaaatagcc gggcgcctcg gcatcagcgc
     3241 atcgtcggct ggggtgcgcc tgcatcgggc ccgggttcga ctgcagcagg ccttggaggc
     3301 gggtaggctg gaagcgccgc gcgcggagcc gcctcctagt gaatagagcc ggtcctctgg
     3361 tccccttcgt agcgtggggc ggaatcagcg tcgcaacgac cccgagcttc ccatccaccg
     3421 agcgtactct actaatggag tgctccaccg ggttcgggtg ggcgtgggcg tgggcgtgag
     3481 cgagtgctct cgatcgcgaa gctggctcgc ctcagcgacg ccccgcggcg atgagcagtg
     3541 gctctgcggc tctgaatgcg cggcccgact gcgcgagagc gaaccagccg gacggagtta
     3601 gccgttctca ccagccacaa gggtcatctt tgtctgcgac ggcccgtcgg gtcgatcggc
     3661 gtgggagggt ggaggcgtcg tgagccctga gagatcgatc cctgggtgta ataaatggcg
     3721 cacgggattc gttctacatg cgggtacggg aatgcccgga tgatgaatcc gcggccactt
     3781 ctccagtggt gatttcatgg cggcgttgaa cgttattacg gatgagactt ccacgcagga
     3841 tttcctcgtc atcaaggatt tcctcgcttc gcagggcatc gatttcggtc gtttccagcg
     3901 aaaccccgcg gtcctcagtc tagctgagcc tcacacccta acggatgagc agcgcgccga
     3961 gttgatcgcg ctctatcccg acgtgcgcga aaaattcgag cgccgcacgg gctttcgctg
     4021 tgatgtggtt tgcttctatc ccgagttgcc acagttgaac ttcattatcg ataaattcgg
     4081 aagtattcat tatcacttcg agactgagta ttggtatttc gttgatgggg aagccaagtt
     4141 cggcttcctg gggacgaacg gaaccaagtt cgagttgacg gttcaaggtg gggagttcct
     4201 tcaagtgccg gaagggtgct ggcaatggtt tcaattgaca cccatcaaac gtatgaagtc
     4261 gatccggtac ttctactcga cggagagcca aaagccccga gtgccttata aattcggagg
     4321 gtcggacgcc caggccggga gcaagcccga ggtggtgggg tcatgacgtt gagttcagcc
     4381 agcgtcacga tgctcgcggc ctgtgcggcg cgggcatcgt ttgcggaccg attgcaagac
     4441 tccttttcca atgagatcct ccggcacctg cctgatgcag acgccaagcg agacacgctg
     4501 gaccaggcgt cgcggggtat gttttcccga tccatcttgc tccgcactct gtatttccgg
     4561 gagcgcttgc gagccacctt acgtcccggt tcacagctgg tgctgctgag ccacgggctg
     4621 gactgcgagc tcatgaagag cggagaacat gccctcttcg cggtggacca tcccgcatcg
     4681 cagcgcttca cccgcgccgc gctggaggcg gccaagattg acgtccgggt gcagttcatt
     4741 gctcatgact ttgcgcgtga cacgccatct cagctgtgga gttccttgcg tgcggcgggg
     4801 ttctcggccg acgcccatag cgtcgtgctg tgggaaggcg ctacctacta cttgcccgaa
     4861 accgcggtgg tccccgtcct gtcatccttc gcgtctcgat gcgctcgagt cgatctattc
     4921 gcagatttcc tgaatcacgg aggctacttc gtgaacgggc gagtcgtcag cccgggcgtg
     4981 ggccagagcc tcgcgttcgt ggagtcgttg ggtgaacctt gggtcggctt cttccagacc
     5041 gacaccttga gtcggacgct gcaggacgcc ggcttccaga gcgtgaattg cgaagatcgc
     5101 agcggagtcg aacagcgcat cctgggagag cgtttgatgc agcccaacat gatgttcttt
     5161 atggaggcac acaaatgacg gaaactgcgc tgagtggtcg agcattcgtc gcgagcttgt
     5221 tgcaggacgt cgacgagacg gtgcagcgga acaagggcgt gatgccgccg ggcatggacg
     5281 gcccggtgct ccccgaacca atcctgcggc attggctgac ctttgccgtg tattacgaga
     5341 aggcggccac ctcgttcatc ggcggatgga tgaggagcac acaggagccc gacgcagtag
     5401 tgcagtttgc acatcagatc gaggatgaag ccaatcactt catttggctg cgcaaacacc
     5461 tgctgtcctt cggtatcgac gcggcgacct tcaagcctcc aaaggaatgg gcgctcctga
     5521 tggaggaatt ctatccgggg ctggaccacc tcgtcgagcg actcgcggcc cacaacatcg
     5581 cgtcggagac gggcgcgctc gggttcatgg agtacaacat ggacaagttt ccggcggaga
     5641 tccaagccac cgtccgcaaa gttgctcgtg acgagaagtt tcacgtggcg atcggcaagc
     5701 gtttgctctc gaagtactgc accacggctg agctgcagga gcgcgcccgc ggggccactc
     5761 gtagcgccct cgacctcatg agtgcggcac gtcgagtctt cgtgcgcgtg tgagcggaga
     5821 gcatgggtag cgaaatgagg cgcacggtca tcactggaat tggagtcatc gccccgaacg
     5881 gccgctcccc gcgggagttt tgggacaacg ccaaccgggg gaggtctttc atcgaggccg
     5941 acccggagat ggaacaactc ggcatcaaga gccgagtcac ttgtcggatt cgcgagttcg
     6001 acccccagga tcatatggac tccgaactcg cgcgggcggt cgcgggcgaa gatcgtttcg
     6061 tccagatcgg tgtcgccgcg ggggcgggtg ctctacgcga tagcgcgtgc ctcgatcacc
     6121 accctgggac gcgggtgggg gccatctatt cttcggccat cgg
//
LOCUS       DASTKU010000724         3842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1256194, whole genome shotgun sequence.
ACCESSION   DASTKU010000724 DASTKU010000000
VERSION     DASTKU010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256194"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(405..890)
                     /locus_tag="VFQ61_25295"
     CDS             complement(405..890)
                     /locus_tag="VFQ61_25295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957848.1"
                     /translation="MKTKYTVLATALTAVLWNAVADAAVPGWAGVPDPMTETQCWLQD
                     GARLKLNTSACGDNAFHNFNVPIPLASDSAGTVTMSWKIGVPPSCSNTVCVAVGETSA
                     RAITFNADGTIHAVGPWTSTSGTKSLAAPAFATAQLQIRMKGNPDNNQSLWAVSGGLT
                     P"
     gene            complement(1718..2635)
                     /locus_tag="VFQ61_25300"
     CDS             complement(1718..2635)
                     /locus_tag="VFQ61_25300"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET9957849.1"
                     /translation="MGPTTPASSAPRKGLPIPVAALAITAIWGFNFVVIKVGTDGVPP
                     LLLAALRFTLLAFPAVLFVRRPQAPWRLVAGYGLFLGVGEFGLLFTAIKLGAPTGLSS
                     LILQAQAFFTAALAALFLDERFSARSALGMLIAASGLALIGWQGDHPGAWGGHFVVAL
                     SMLLAAAFMWAVANILAKRIGRVGPLNLLIWSSLFSPMPLLVLSFAYEGPGAMVAAIT
                     SLSWLSVGALAYLVVLATLVGYGGWNHLIVEHGAARVAPYSMLVPIFGMISGAVVLHE
                     TFNRYHASGAGLVLLGLAVHALGGRLYER"
     gene            complement(2704..3609)
                     /locus_tag="VFQ61_25305"
     CDS             complement(2704..3609)
                     /locus_tag="VFQ61_25305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957850.1"
                     /translation="MVDRKLGGGSLLIAWAVLASACGTQSSGDSSGGSAGTAQGGRAS
                     GGAPAFGGSGGGGFASGGTGNAGSASGGTNGGGDGGAQNGGAGTGSGGTATGGTSTGG
                     VTASLGGSGAGGKSTGGSSNGGKGSGGTTSGGTNTGGAHSGGTSAGANSGGRSAGGTS
                     GQSGGGGTAGKSAAGGTAGTGSGSGGTAGKTGMGGTAGTGSGSGGTAGGGGVTWCGNV
                     QCDCTLNGIPLYGKVQITTSAFADFDIEVVTAFADLDVQKVTSFANDCGEWEIVTSFP
                     DFTVREVSSFGDFKVRYVTAFPGLP"
BASE COUNT          714 a         1277 c         1198 g          653 t
ORIGIN      
        1 cggcgggcaa cactcaatgc gtgagttacg tgctgcgcag cattcagcac gcagtatgcg
       61 tatcaggcgc tctcgaaatg cttcttcccg aagcggtatg cccactcggg agcaacttga
      121 tctcagtaag ttctgtgccc gcccgcgagc actaccgggc cgccgatgcg gttcagcgcg
      181 ctcggccgca ttcgaagcgg gcgatggctc gagagcatgt gcgaggctca gggcagcgcg
      241 accagctcgt tcgcttcgac cgcgcagaag ggcgcatccg aaacttggcc gactgcgccg
      301 ccctgcccgc cggcggtgct gtgactgacg aagaccgatg ccatgccgag ctcgcagcta
      361 tcgtggcttg cctctcgggg attgggtgtg ccccgaaaaa actactacgg cgtcaagcca
      421 cctgacaccg cccagagcga ctggttattg tccggattcc ccttcatgcg aatctgcagc
      481 tgagccgtgg caaaggccgg agcggcaagc gacttcgttc cgctcgtcga ggtccacggt
      541 cccacggcgt ggatggtgcc gtcggcgttg aaggtgatcg cacgagcgct cgtttccccg
      601 acagcgacgc agaccgtgtt gctgcaacta ggggggacgc cgatcttcca gctcatcgtg
      661 acggttccgg cggagtccga tgcgagcggg atgggaacgt tgaagttgtg gaaggcgttg
      721 tctccgcaag cgctggtgtt caacttcagg cgcgcgccgt cttgaagcca gcactgggtc
      781 tcggtcattg ggtcgggcac gcccgcccaa ccgggtaccg ccgcgtcggc aactgcgttc
      841 cagaggacgg ctgtgagtgc agttgccaga accgtgtatt tcgttttcat gcgctcttgc
      901 tctttctcgt ttgcggagaa cgacaagcat cgaatcaatg tatgcgcccg tctccgaaac
      961 aatcgaggca cactaaccta gagttagaaa ttgcggaagc ttctcgcacc gcgccgcgcg
     1021 tggcggtgcg agggcttcct catcgtggag cgctggttcg ttgcgccggc gcgctcgggt
     1081 ggggccgcca gcgtccgcaa gctcgttcga cgacctgtgg gagttcagcg acaagcgcct
     1141 gtgcagtgcc cccacgtccc atcatcgagg caaacacacg aagagcaacc ttgcacggca
     1201 atgcttccaa cgctgaaaga ggcgccgtcg cgttcgcaag tcactgtgtc gggggtggga
     1261 tcgggcggcg gctctggaca cgcgcccgtg cagcgacccc acgtcccgtc ggattcacaa
     1321 atgcagctca cacagcctga gcgatagctc tcccccgcag agtatttctg tccgtcgtgc
     1381 tcgcactgga ggccgagctg ctgcgggtcg gacccatgcg gctcagcgtc gccacaagcg
     1441 acccaagccg gcaccgttgc tagtacgaac gcaaccccaa atcgcttcac gctacacctc
     1501 gcagtctcag tgaacggcag ctcgaagtgc aagtcggatg ccacttgccg atgaccactt
     1561 tcatcgcgca atcctcgacg caaaaccact cgtcagtggg tcgtgttgtg ccagtatgcg
     1621 ccgggcatcg ctccgcgtgt cttgcacgaa gctgtgctgc acgaagctgt gctgcacgaa
     1681 gctgtgctgg gccaagctat gctgcggctc tagcgctcta gcgctcgtac aacctaccgc
     1741 cgagggcgtg caccgcgagg ccgagcagca ccaaaccggc acccgaggcg tggtacctgt
     1801 tgaacgtctc gtgcaggacg acggcgccgg agatcatacc gaagatcggt acgagcatcg
     1861 agtagggtgc gacgcgcgct gcaccgtgct ccacgatcag atgattccat ccgccgtagc
     1921 cgacgagggt cgctagcact accaggtagg caagtgcacc aaccgagagc cacgagagtg
     1981 aggtgattgc cgcgaccatc gcgcccgggc cttcgtaggc gaaggacaac acgagcaacg
     2041 gcattggcga gaagaggctc gaccagatca ataagttcaa tgggccgaca cggcctatgc
     2101 gcttggccag gatgttagcg acagcccaca tgaaggcggc cgcgagtaac atcgagagcg
     2161 cgacgacgaa gtggcccccc catgcgccag ggtggtcgcc ctgccaaccg atgagcgcga
     2221 ggccgctagc ggcaatgagc atccccagcg cgctcctcgc gctgaagcgt tcgtccagaa
     2281 acaacgccgc gagcgcagcg gtgaagaagg cctgggcctg caggattagg gagctcagcc
     2341 ccgttggcgc gccgagtttg attgcggtga acaggaggcc gaactcgccg actcctagga
     2401 acaagccgta gcccgcgacg agtcgccacg gcgcctgcgg ccgtcgcacg aagagcacgg
     2461 ccgggaatgc aaggagcgta aagcgcagcg ccgctagcaa caggggaggc actccgtcgg
     2521 tgccgacctt gatgacgacg aagttgaacc cccaaatcgc cgtgatcgcc agcgcggcga
     2581 ccgggatggg caagccctta cgaggagcgc tactggcggg agtcgttggg cccaccgtgg
     2641 gatgttgcgc cgcgagggga gggccgtgtc aagcgcgcat ctctgcgcaa cgcaagcgcg
     2701 accttatggc agccccggaa acgccgtcac gtagcgcact ttgaaatcgc cgaacgagct
     2761 gacttcgcgc acggtgaagt cggggaagga cgtgacgatc tcccattcac cgcagtcgtt
     2821 cgcgaatgag gtgaccttct gcacgtcgag atctgcaaag gcggtcacca cctcgatatc
     2881 gaagtcggcg aacgcgctgg tggtgatctg gactttgccg taaaggggaa tgccgtttag
     2941 cgtgcaatcg cattgcacgt tgccgcacca cgtgacaccg ccacctccgg cagttccgcc
     3001 tgagccgctg ccagtccccg cggtcccgcc catgccggtc ttgcccgcag ttccgcctga
     3061 gccgctgcca gtccccgcgg tgccgccagc tgcgctcttg cccgcggtgc cgccaccgcc
     3121 actttggccg ctcgtgccac ccgcgcttcg tccgcctgag ttggcgccgg cactcgtccc
     3181 accagagtga gcgccgcccg tgttcgtgcc gccagaggtg gtgccaccgg aacccttgcc
     3241 accattgctg ctgccgccgg tgctcttacc gcctgctccc gaaccaccga gactggcggt
     3301 gacgcctccc gtagaggtgc cgcccgtggc ggttcctccg gaacccgttc ccgccccacc
     3361 gttctgcgcg ccgccatcgc cgcccccgtt cgtgccaccg ctggccgagc cggcgttacc
     3421 agtgccgccg cttgcgaatc cgccgcctcc gctcccaccg aaggccggcg caccgccgct
     3481 agcacgaccg ccttgagctg taccagccga gccaccgctc gaatcgcccg agctttgggt
     3541 tccgcacgca gaagcaagta cggcccacgc aatgagcaga gaaccaccac cgagtttcct
     3601 atccaccatg cccccttcta cgccgaatct cgcccacttg acgacagttc tttgcgagca
     3661 gggcgaagac acggcgccga aacgcggtcg cgtgtgaaac gatggcggta aagcgggagc
     3721 agccctccag tcataatcgc gtcttcgatg ctgcgttccc gtccggaagc aacagatctt
     3781 ccaagaagaa cgcggcgagg tcgttacgcc cggagagtcc cgcctttttg taaacggcgc
     3841 gc
//
LOCUS       DASTKU010000725         8267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1480709, whole genome shotgun sequence.
ACCESSION   DASTKU010000725 DASTKU010000000
VERSION     DASTKU010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480709"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..1196
                     /locus_tag="VFQ61_25310"
     CDS             408..1196
                     /locus_tag="VFQ61_25310"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9957851.1"
                     /translation="MHTAGRRGATRVVSYGFMRTPAPSLEAPAVYPVEEPGGEDILQC
                     WMLEVLRPLIERWLAERGTPWLVGSDQFIYFEKGQISKRIAPDIYILPGVPPDTRIPS
                     WKIWETGIRPSFALEICSQDWQKDYITAPQLHNQIGTEELIVVDPDYLKRREGYRFQR
                     FERTAHGLERQECTQADRVRSRLLGCFVRLVGTEQEPRQHVRLALDPEGDVLFPTQEE
                     AERLRANTESQRAEEERKRAQEQKQRAEAALARVAELEALLKQR"
     gene            complement(1362..3050)
                     /locus_tag="VFQ61_25315"
     CDS             complement(1362..3050)
                     /locus_tag="VFQ61_25315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957852.1"
                     /translation="MHVRHPHEDAFILFRYVEHLAHGQGIVFNTVGPHAEGATDFLWM
                     VLLALGTRLGVDVAEFAALLNALGVALLAGLGSAMFLGLRAPSLGDRAFLLILSMALP
                     FLPFASASYEGFSSLLYCGLIVGGAAAAVSEERKWVHATPWLALIVALFRPDGVLLAV
                     PMTVTALWRSRARLHASKRLLFHVLAAAVLGACYFMWRYRYFGLLLPLPLYVKSRGTA
                     VLPGLEDNWDWFWNASGAPLILLCCITLALVVQRRGSGAHRSRSLLWIALPAVFLFAG
                     LLFAHQSQNIAFRFQAPIHALLLALMLSQLGVALDHWPSHLARGSGRFFGVLAIVAAF
                     FFPKTIWPLDYMDGVSVELARHLNGKVVAATEAGRLAFWTDGTVIDVVGLNDPRCAQR
                     PADLNYISAVQPDVIMFHAAGTLSERLLRRALGSASRGEVGKIPAQALGAALQPSFSR
                     LVESAPREYPGSVLPHRLAAATLAYWLSRSSEYEVVLLRYGGSFRHVWAVRKSLPDAN
                     ILLARLEAAPRAPYWPYSNFRRLRLGRLACTAMEWPVRILGAAAFGWPRAPSCP"
     gene            complement(3260..4630)
                     /locus_tag="VFQ61_25320"
     CDS             complement(3260..4630)
                     /locus_tag="VFQ61_25320"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9957853.1"
                     /translation="MGSTRLTRARELGQHGIALLLGHSIPICLVLAVGLASKRTIWPF
                     ANALLVIPLVSMTVTAHEASHAFAGILLGMRPSRVALGFGPKLFEIAIKSCRIQVHAV
                     LNGGMTVLANEAQLSWVRLRFWLTVAAGPAFNLLLLMLLLPLHARHESRGLAIGWAPV
                     QVAWFVALFAAAYTLIPFRSFSSSGDIRNDLWLLLAVPFWREEQVAELKTSALLERAA
                     AFERQKRFDEAFACFDQALAQVPNSYGARLARAALLIRRGQLEQGRKDTVALLTCKEP
                     WPAYRLGALNNIAWADLLLDRPELVEEAARYSSEVVIAQSRVSPYQGTRGLCLVTLGD
                     WEEGMAALRYAFGTNLEAENRALNACSLALGLMRVGKPADARQWIETARSLDSSCLLL
                     ARAEGEVEGRVPCPEPIPRKLRVSLAREHQAAAERARERQHARAQVYWAAVFVGTLAV
                     LKFIFG"
     gene            complement(4643..5203)
                     /locus_tag="VFQ61_25325"
     CDS             complement(4643..5203)
                     /locus_tag="VFQ61_25325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957854.1"
                     /translation="MNLRAVLLLAISSAACAPVASLRAPYSFPEERRFELGGGVASLG
                     KRPYVEESTQFTGQVWGTYQPRPWLAVSAIGAFDDSAAAGGLAARVHALRSDRLALSP
                     EVEAGYAWVATSLGLAARVFEHNWLYASPRVGNLGSAWTVFIPVGVNLELYRGLGVRG
                     EASLSYAELKYYNLRRHFAAAVFYQF"
     gene            complement(5200..6561)
                     /locus_tag="VFQ61_25330"
     CDS             complement(5200..6561)
                     /locus_tag="VFQ61_25330"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HET9957855.1"
                     /translation="MAGLLTIGTSYYWIRPQLNQIDWDFPDAVTRIRNLRASLDSNKF
                     KTNHVLHPLAGSSYYLFAREVDLSPLAAAAYSFGSSAVFEYLLEWLEKVSINDLIFTP
                     GAGIPAGEFVYNLSNYLNSAPAETSWAQDIASYSLGLPSRVHGRPRAKFDSRAVARLP
                     RDRLGFSSAYHHEFHVAIGVGTVDTEAGYGGLYQRLTLHGEIVAMPGFQRPGRFAQSF
                     GQGNFSETRFRYSISDHGMEADAYFQATLFGYYAQNYRDDGRGLTGSAGMLGLGLGYR
                     YHDSQPEGPIDQYAFAHLLGVNSDLWLARRGAVLRLRSDAHLDFGAVRSLPYAEHVAP
                     FDTSGMKQVLRDQGYYFVRGASARLETSLSVFGLQLGASYGLGRYYSIQGIDRFQGEV
                     LYDISSNDTIQDLDAWFAGAAPHSPIELRFDYNLTRHVSQMPPWTDRHRDQRATFSLG
                     LRL"
     gene            7356..>8267
                     /locus_tag="VFQ61_25335"
     CDS             7356..>8267
                     /locus_tag="VFQ61_25335"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9957856.1"
                     /translation="MNGWTLKRGAQVLVASFALMALAGCNKAKSGASAAGPKTSGPAA
                     SGSAPSATAKRTSCDDYADKICAKAGAESPTCTGFKEATKLMSTSACSAGLKDISVSL
                     SKMDAARSECDKLVKTLCDAIGPKTKTCEMVTTQTKQFPPERCTAMLEHIPEITADLQ
                     KMEAANKPLTPELQAAIVAGGPPSFGPADAKVTLVEFSDFECPFCSRAAKVVHDIRAK
                     YSDKVRFVFRQFPLPMHSHAHLAAEAALAANEQGKFWEFHDKLFENQRALDRAALESH
                     AKDAGLNVAQFKKALDDKKHNAQVDEDM"
BASE COUNT         1793 a         2516 c         2573 g         1385 t
ORIGIN      
        1 aaatccacgc ttcgggatcc agaatctcag cgcgcgatct gtcgaaaccg gcggccatct
       61 acttcgctga gtcttcagta ggcggcgggg tatcaaatcg atccttcgcg taatccgcac
      121 cttgatctgc atgcagttcg caaagcaagc gtcgtactcg gggtggcaaa caggtggtga
      181 ggggtacgct cccaagagtc tgagagtcga ggcggtcgcg agtgcgtgtc tccatgacac
      241 cggatcgcgg agacttcgtt ggcgaccgcg aatgatgaac tcgcccgtga cggcactcgg
      301 gatatcccta atgccgccgt tcgaatctcg ggatgcaccc cagttcctgc gggattttgt
      361 gctcttttgt tcaggcgttc tcgatgtctc gtgctacatg gcagtgcatg cacactgccg
      421 gtcggcgggg cgccacgcgc gtggtatcgt acgggttcat gcgcactcca gccccttctc
      481 ttgaggcccc ggcggtgtat cccgtggagg agcccggggg ggaagatatc ttgcagtgct
      541 ggatgctcga ggtgctgcgt ccactcatcg aacgatggct ggctgaacgc ggcactccgt
      601 ggctcgtcgg ctccgatcag ttcatctact tcgagaaggg tcagatctcg aagcgcattg
      661 ctccggatat ctatatcctg cccggtgtcc ctcccgacac tcgaatccca tcttggaaaa
      721 tctgggagac cggcattcgt ccgagcttcg ctttggagat ctgctcccag gattggcaga
      781 aggactacat caccgcgcct cagctccaca atcaaatcgg aactgaggag ctcatcgtgg
      841 tcgatcccga ctacttgaag cgccgggagg gctatcgctt tcagcgcttc gagcgaactg
      901 cacacggtct cgaacgtcag gaatgcactc aagcagaccg cgtgcggtcg cgactactcg
      961 ggtgtttcgt gcgcctggtc ggcaccgaac aagagccgcg gcaacacgtc cgcctggccc
     1021 tcgaccccga gggtgatgtt ctgtttccca cgcaagaaga ggccgaacgg ctccgcgcga
     1081 acaccgagag ccaacgcgct gaagaggagc gcaagcgtgc ccaggaacag aaacaacgcg
     1141 ccgaagccgc attggcccgt gtcgccgagc tcgaggcttt gctcaagcag cgttgagccc
     1201 ctcgtgacgc gctcaagccg aattagcgat catccaacaa gcagaagtac tccccggatc
     1261 gctctcccaa tccactcgag atcatagtac catgtgggtt cgagagggcg ctggcacagg
     1321 atgctcgtag acacgccagt acggcgtctc gatctcgcag cttacggaca cgacggcgct
     1381 cgaggccacc cgaaggccgc cgcgcccagt atgcgcacgg gccactccat ggctgtacaa
     1441 gcgagtctgc cgagacgaag tcgcctgaag ttggagtacg gccaataggg agcccgtggg
     1501 gccgcttcta aacgagctaa gagtatattg gcatctggca aactcttacg caccgcccag
     1561 acgtgacgga atgagccccc gtaacgcaag agaacaactt cgtattcact ggagcgactc
     1621 agccagtacg cgagcgtcgc ggccgcaagg cggtgtggaa gaacagaacc ggggtattcg
     1681 cgcggagcac tctcgaccaa acggctaaag gaaggctgca gcgcggcccc gagtgcttgg
     1741 gccgggatct tgccaacctc gccgcgagat gcactcccga gcgcacggcg caacaaacgc
     1801 tcgctcagtg tgcctgccgc atgaaacatg atcacgtcag gctgaaccgc ggagatgtag
     1861 ttgagatcgg ccgggcgctg agcgcagcgc ggatcattga gcccgacgac gtcgataacg
     1921 gtcccatcgg tccagaacgc gaggcgccca gcttcagttg cggccacgac tttcccattc
     1981 aggtgtcgcg ccaattcgac gctcacacca tccatgtaat ccagcggcca tattgtcttg
     2041 ggaaagaaga aggccgcgac aatggcgaga acgccgaaaa atcgaccaga cccgcgcgcg
     2101 aggtgactcg gccaatgatc aagagccacc cccaattggc tcagcatcag ggctagcaac
     2161 aaggcatgaa tcggcgcttg aaagcgaaag gcgatgttct gcgattggtg agcaaacagc
     2221 agccccgcga acagaaacac cgcgggtagc gcgatccaga gtaatgaacg tgaacggtgc
     2281 gcccccgaac ctcgccgctg aaccactaag gctaatgtta tacaacacag cagaataagc
     2341 ggcgcaccgc tcgcgttcca aaaccaatcc caattgtctt cgagcccggg caatacggca
     2401 gtaccgcgcg acttcacata gagcggcagc gggagcagca aaccgaaata ccggtaacgc
     2461 cacatgaagt agcaagcgcc caacaccgcg gccgcaagca catgaaatag caaccgcttg
     2521 gacgcatgca gtcgcgcgcg cgagcgccac aatgcagtca cggtcattgg cacggcaagg
     2581 agcactccgt ccgggcggaa gagtgccacg atgagcgcaa gccacggtgt cgcgtggacc
     2641 cattttcgct cttcgctgac ggccgcagcg gctccgccaa cgatcaatcc gcaatacagc
     2701 aggctgctaa acccctcgta gctggcactg gcgaagggca agaacggcaa cgccatgctg
     2761 agaatgagta gaaacgctcg gtcacccaag ctcggggcgc gaagaccgag aaacattgcc
     2821 gaacccaggc ctgccaatag ggcaacgccg agcgcgttca agagagcggc aaactccgct
     2881 acgtcgacgc ccaggcgggt gccgagtgcg agcagcacca tccacagaaa gtctgtcgct
     2941 ccctccgcat ggggacccac cgtgttgaac acgatgcctt gcccatgtgc caagtgctcg
     3001 acgtagcgga acaggatgaa cgcatcttca tgcggatgcc gaacgtgcaa gtgagcaagc
     3061 gcccggcaca agaagatgac gctggagagt attgctgtgc aggcagcggt aacccagcgt
     3121 tcagtccggg cctggaaagg cgattgcgct ccgcttccag accacttctg gtcaagccca
     3181 agttggcgtt tcactcagcg ctgccagtta taccgagcgc cggcaagtgt cgagccccgt
     3241 tcgacccctc ggcgtgcgct cacccgaaga tgaacttgag gacggccagg gttccgacga
     3301 agacggcggc ccagtagact tgtgcgcgcg cgtgctgacg ctcgcgcgct cgttcggcag
     3361 cagcttggtg ttcacgggcg aggcttaccc tgagtttgcg gggaatcggc tcggggcacg
     3421 ggactcgacc ctcgacttca ccctcggccc gcgccaatag taggcaactc gagtcgaggg
     3481 aacgcgcggt ttcaatccat tggcgcgcgt ccgcgggttt gcccacgcgc attagcccta
     3541 acgcgaggga gcaggcgttc aacgctcgat tctcggcctc caagttcgtc ccgaatgcat
     3601 agcggagtgc cgccatcccc tcttcccaat caccgagagt cactaagcag aggccgcgcg
     3661 tcccctggta gggtgagacg cggctctgag cgatgaccac ctcgctggag tatcgggcgg
     3721 cctcctccac gagctcggga cggtcgagca gtagatcggc ccacgcaatg ttgttcagtg
     3781 cgccgagtcg atacgccggc cacggttctt tgcaggtcag gagggctact gtgtccttac
     3841 ggccttgctc gagttgccca cgccgaatga gcagcgccgc gcgtgccaag cgagctccgt
     3901 acgaattggg cacctgggcg agcgcttggt cgaagcaagc gaatgcttca tcgaagcgtt
     3961 tctgccgctc gaaagctgcg gcccgctcga gcagcgcaga ggtcttgagc tccgcgacct
     4021 gttcttcgcg ccagaagggt accgccagga gcagccatag atcgtttcga atgtcacctg
     4081 aggatgaaaa cgagcgaaac ggaatgagag tgtaggccgc cgcaaataaa gccacgaacc
     4141 aggcgacttg aactggcgcc cagccgattg cgagccctcg actttcgtgg cgcgcatgca
     4201 gaggaagcaa gagcatgagc aggagcaggt tgaatgcggg gccggcggca accgtcaacc
     4261 aaaaccgaag ccgcacccag ctgagctgcg cttcatttgc gagcacggtc atgccgccgt
     4321 tgagcacagc atgcacctgg attcggcagc tcttgattgc aatctcgaag agtttgggac
     4381 caaatccgag cgctactcga gacggtctca ttcccaagag tatgcccgcg aacgcgtggg
     4441 aagcctcgtg agcggtgacc gtcatactga cgagggggat cacgagcagt gcgttcgcga
     4501 acggccaaat cgtgcgtttc gatgccagcc caacggcgag taccaagcag atcgggatcg
     4561 agtggccaag cagcagggct atcccgtgct gcccgagttc gcgggcccga gtgagtcggg
     4621 tgctccccat gagtctcatg agctagaact ggtaaaagac cgcggccgcg aagtgcctgc
     4681 gtaaattgta gtacttgagc tccgcgtagc tgagcgatgc ttcaccccgc acgcccaagc
     4741 cccggtagag ctcgaggttg acccccaccg gaatgaacac ggtccaagcg gatccgaggt
     4801 tgcccacccg gggagacgca tacagccaat tgtgctcgaa gacgcgagcg gccagcccga
     4861 gtgaggtggc cacccaggcg tagcccgcct ccacttccgg gctgagcgcg aggcggtcgc
     4921 tccgcaaagc atgcacgcgc gcagccaagc caccggcggc cgccgagtcg tcgaaggcgc
     4981 caatagccga gaccgcaagc caaggacgcg gttggtaggt gccccacact tgcccggtga
     5041 actgcgtgct ttcctcaacg tagggtcgct tgcccaggct cgccacgccg cccccgagct
     5101 cgaagcgacg ctcttcggga aatgaatagg gcgcgcgcag actcgccacc ggcgcacacg
     5161 cggccgagct gatcgccaga agaagaaccg ctcgcaggtt catagccgta gccccaggga
     5221 gaaagtcgca cgctgatcgc ggtgccgatc cgtccaagga ggcatctgac tcacgtgtcg
     5281 agtcaaattg tagtcgaaac gtagctcgat ggggctgtga ggagcagctc ccgcgaacca
     5341 agcgtccaga tcctgaatgg tgtcattgga ggagatgtca taaagcacct cgccttgaaa
     5401 gcgatcgatg ccttgaatcg agtaatagcg cccgagcccg tagctagctc cgagttgcag
     5461 gccgaagacg gacagtgacg tttcgagacg agccgatgct ccacgcacga agtaataacc
     5521 ttggtcacgc agcacttgct tcatgccgct ggtatcgaat ggggccacgt gttcggcgta
     5581 gggcaatgag cgcaccgctc caaaatcaag gtgggcgtcg ctgcgcaggc gcaagacggc
     5641 tccgcgacgc gcgagccata ggtccgagtt tacgcccaag agatgcgcaa aagcatactg
     5701 gtcgatgggg ccttcgggct gcgagtcgtg gtagcgataa ccgagcccca accccaacat
     5761 gcctgccgac ccggtcaaac cgcggccgtc atcccgatag ttctgcgcgt agtaaccaaa
     5821 aagcgtagcc tggaagtagg cgtcagcttc catgccatga tccgaaatcg agtagcggaa
     5881 tcgcgtctcg ctaaagttcc cttgcccgaa gctctgggcg aagcgcccgg gtcgttggaa
     5941 gccgggcatc gcgacgatct cgccatgcag ggtgagccgt tgatagagcc cgccgtaacc
     6001 ggcttcggtg tcgaccgttc caaccccgat cgcaacatgg aattcgtgat ggtaggcgga
     6061 gctgaagccg agcctgtcgc gtggcaagcg agctaccgca cgcgagtcga acttggcacg
     6121 cgggcggccg tgaacgcggc tcgggaggcc caagctgtag gaggcgatgt cttgggccca
     6181 gctcgtctct gcaggagcgc tattcagata attcgaaaga ttgtaaacga actcaccagc
     6241 gggaatgccg gcgccaggtg tgaagatcag atcgttgata ctcactttct ctagccactc
     6301 gaggagatac tcgaatacag ccgatgagcc gaacgaatag gcggctgcgg ccaaagggga
     6361 aagatccacc tcccgtgcaa acaggtaata ggatgaaccc gcgagcggat gcagcacgtg
     6421 attcgttttg aacttgttgc tatcgagtga ggcccgcaag ttgcgtattc gcgtaacagc
     6481 gtccgggaaa tcccaatcga tttgattgag ttgggggcga atccagtaat aggacgtgcc
     6541 tatggtcagc agtccagcca tctccgctgc gccaagccag tagttgggcc gccggtgacg
     6601 gccgccaaac catgcgtcat agcttgcagc ggcgcggcgc ggattcccat ctgtatcgat
     6661 gaaacacccc gtctcgaccg aacgcgtgca atggcggctt gccggtacgg gcgaggaggc
     6721 gtgaagcggt tgagaaagcg ccagtcgaac ggagcccacc gagtacgcga tcactacgca
     6781 gcatcccccg aaccagcgga gtgcacgcga gtacgacagc gatgcaagat ggaccacgct
     6841 gaatacgttg ggtgcaaagt gcgatccagg gagttgccgt gcccaaagct gattttctca
     6901 gcaatacgca ggcattcgtg ggtctatcga agcgctgcgc cacccccgaa tgggcacagc
     6961 gcaaggcttg cgtctgcggc ctctgcccgc gattgatctg cgacctcgcg cgacaacgaa
     7021 gcgactgaag ttcgcgagtc atccggcaga gccaggagca gagctgctgg gcgagctgac
     7081 gagctcgcac gctcgagcga gtacgtctgc gcgagactga atgcccctcc gggcaacgcg
     7141 ggagatgacg gcgcgcagcg gggtcgtttt atcgcgcctt gggccccgta gggccgtgac
     7201 ggcaggcgaa aacgtgaacc tgcttgggag ccggcagccg cggagccggg tcgttttcgg
     7261 gcgaagcggg agtggcgtgt ctctgcggca ggccagtgct aagcctgcgg gcgctcggac
     7321 ggctgtcgtt tgggcggaca agaggaatga attcgatgaa tggatggacc ctgaagcgcg
     7381 gcgcccaagt gttggtggcc tccttcgccc taatggcact cgccggatgc aataaggcca
     7441 aatccggagc atccgcagca ggccctaaga cttccggccc cgccgcctcc gggtcagccc
     7501 cgtcggcgac cgcgaaacgg acctcgtgcg acgactacgc agacaagatc tgcgcaaagg
     7561 caggcgcgga gtcgccgacg tgcacgggct tcaaagaggc caccaagttg atgtccacct
     7621 cggcgtgcag cgccggactg aaggatatct cggtgtcgct cagcaagatg gatgcggcgc
     7681 gctcggagtg cgacaagctc gtcaagacgc tatgtgacgc gatcggtccg aagacgaaga
     7741 cctgcgagat ggtcaccacg cagaccaagc agttcccgcc cgagcgctgt acggcgatgc
     7801 tggagcacat tccagagatc acggctgacc ttcagaagat ggaagccgcc aacaagccgc
     7861 tcacgccgga gctgcaggcc gcgatcgtgg ctggaggccc gccgagcttc ggacccgccg
     7921 acgcgaaggt gacgctggtg gagttctcgg acttcgaatg tccgttctgc tcgcgcgccg
     7981 ccaaggtggt ccacgacatc cgcgcgaagt acagcgacaa agtgcgcttc gtgttccgcc
     8041 aattcccgct gccgatgcac agccatgccc acctggccgc ggaggccgcg ctggctgcca
     8101 acgagcaagg taagttttgg gagttccacg acaagctgtt cgaaaatcag cgcgctctcg
     8161 atcgggccgc gctggagagt cacgccaagg atgcggggct gaacgtggcg cagttcaaga
     8221 aggcgctcga cgacaagaag cacaatgccc aggtcgatga ggacatg
//
LOCUS       DASTKU010000726        29687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1927648, whole genome shotgun sequence.
ACCESSION   DASTKU010000726 DASTKU010000000
VERSION     DASTKU010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 29687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29687
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1927648"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFQ61_25340"
     CDS             complement(<1..362)
                     /locus_tag="VFQ61_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9957857.1"
                     /translation="MKSDLSDSIYVRGAREHNLKNVELRIPKHRLVILTGVSGSGKSS
                     LAFDTLYAEGQRRYVESLSSYARQFLGQMEKPKYDMIRGLSPTISIEQKSASSNPRST
                     VGTVTEVHDYLRVLFASIG"
     gene            complement(598..1431)
                     /locus_tag="VFQ61_25345"
     CDS             complement(598..1431)
                     /locus_tag="VFQ61_25345"
                     /inference="COORDINATES: protein motif:HMM:NF012255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HET9957858.1"
                     /translation="MASYDASRGCYVIRVVYDGPGYAGKTTNLERISQMVPATRRSEM
                     YTPAALKGRTMFFDWLEVDGPKHFETSLKFQLISVPGQIERNYRRKPLVEMADVLVFV
                     CDSSVQKMPDSLRCFARLRSSIKQRETPVPLVVQANKQDTPGAASLEEIAARLGLAAE
                     VPLVSAAACTGAGVQETLRWAARFGVDGLRKSGNVDLLPPEFANADALFDHVLGFEDV
                     PNPNAVVEVEEVDVHASYAPVNEAALAAHLSAASMDSLLFRARKAAARNPEEPEAPPA
                     E"
     gene            complement(1555..6864)
                     /locus_tag="VFQ61_25350"
     CDS             complement(1555..6864)
                     /locus_tag="VFQ61_25350"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9957859.1"
                     /translation="MVVESLSAELERLYELEELKALCSSLLGMDPSEVGGASAKGSFA
                     RALSQKCVEVEAVDALIDVVLASRRELPQELVKKLRNGAIDASERPQPGDELGEYLIV
                     GELGTSASGSVYRARKNAEDLRVRRLSRELRTRRRDVQRYLAATRVASNATHEGLPSL
                     VAAGALEDSGRLFGVAHTFREGEPLAKWISDRGGRHINELLPLFWALLDALKAVHAQG
                     LVHGALHAGNVLVLEGANSRPEIVLLDAGAHHLRPGLLGLARSGSANWLASASPELLR
                     GDALDARTDVYALGTLIYQTMSGRAPFVGSSPVDVALGHLTKDAESLSFAAAGNGATP
                     EVDVFVKLLMEKDRALRPRDATEVEEGLRRLWRASQRPPSSVADELVEERFQALTDVA
                     WDEQKASEVEALVDLGVPPLRLADGFYSVAREVRAKNTVGSDRIVRKLLARAARLYEM
                     ADRHEAAEKLYQGLVKLDPEDTQVVESLDRLRKRLGKFDELIESLIERSDAAKVATER
                     ARYFAEIARIYSNEVKDPEQALVAATQAFCFDPGNEKLSEQVEKLAGSRYPDWEEVFS
                     HCLEAMESDLADDAKAALAFQIGRWYSEKVSRPDLALPWLTRSVELEPAHDRALAELA
                     QLYRKAQQWSELGQTLMRRADIAPPNQARDLRAEAADLLVNQLSNMQAAQDAYESILA
                     EDPGHARAAEGFSRLLKAQGDTARALRVLEARAEVLSGEERHKSLLEIAETYEHELDK
                     LADAERAYRQILKDNPKHLDALKGIDRLLTRAARYEDLIETLEAELELAVTARQKIAL
                     HERIAKLYDEEFLDHAQAARALEAILELDPSRESAATDLARHLRVLERFEDLAGLYEK
                     QLSLESGSARRVDLGLQLGRVLGEQLKDLPRAIAAYERVLELAPSHAGALEALANLRA
                     ASGDRESAVRAVEELAERATTPEARAEQYLRAAALLEEQGDNTGALLRYKLAADACPT
                     NAAITRKVREKYLEHKNYAAAVQLIEEELERTEGSHARAKLAGDLAVLCQRHLKDDKR
                     ASAAAQIALHLDPTNPEASRVQGRLAYAEERYVEAAKRLEPSIPGLVTLDAEEAAETA
                     FVYIDSLAESGMTEKAVAAVGDLLELLAKDAQALLAVSELTAAHGSPERALAVCDLLL
                     ERHDSELGGSELGAVYRRRGEALFKLGREREAVEALERAIRADSSDAASFRALGKVYA
                     ARDEWERVIETRYRELELVQGDERVQTLFEIGDIAASKLNNPDYAARALLLALDERPN
                     DRKILAKLMQLYSAEKDWPQLIEVISRLAEVVDDPKQRAKYLHTAAMLAARELRDPTR
                     AIEFVDLALESDPENEPAFNEGLKLRRQVRDYEGLKEMLKRRAQDLAPREDKGDLLGV
                     LEQLAEIYEQHLQRHDQAVRVYESMLELDRDNPRYQEKISQLYSAEPTQYFEQAVGSL
                     TAWVKRDPYQPTPYKMLRKVYTEARRADGAFLACQALHVLGQAEPDESRFFSRMRDTD
                     PPAIQRSLSAPEWNELIAPEGAEPLLTQLFALVEPFVVKARAEPLEAFGLAEDHRIDA
                     ESYPYGLVLATHYAAEALGIPEPPMYQRPEDPGVLGFLPTLPPAILVGGGAFNANFES
                     LQAAFIAGHHIAYYQPGLYLRQLLPNLTALKAWLFAAIRLVKPRFPVTPDLEAPVADS
                     SKVLARLATGAGIEQLTHVVTKLLSSETALDLKRWVHAVDFSADRAGLAVCHDLETVG
                     ALIQSVPSAQGSPSVEARLENLMAYSVSERYVELRSRQGVSLD"
     gene            complement(7105..9687)
                     /locus_tag="VFQ61_25355"
     CDS             complement(7105..9687)
                     /locus_tag="VFQ61_25355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957860.1"
                     /translation="MRILLSCMAGAALCLASCSKPPESLPPLPSRARPKAPLRDPGVP
                     SLHATEIGRVPGATYGPYLAARADGGLVVWAANEGNERRFQSVALSATGRPLKAAQLL
                     APAPAEMGVVLVRPLSSGYALLYTRQEPSREETLEALCTSAEGLTAKDQKPATTSLPA
                     RALWVEAVPVAGGALVFYATRSNNARAAEVRVRRLDASCRFGEAELLEGQALAWQATA
                     FAEGAQLTAMRPGTGNNRSVWSTSVTNDGKIKRSVTVVGGAEVELDLDAAYVDSHLLV
                     AFTDHRSLDPHISIASLDSDQKLVREPTPLTAKEGEQALVRLVSEYPGAKQGYVAFER
                     FTGTEPGRHISLSALDGEGRLKGRRVELAYDREDGIPDFVASAGGLAVVTSTKPCPKR
                     GECGDQPVVPLFVSFDAALTPLASEPLRLDALQGKVAELAVGLACTGSGCFGVAALTR
                     VPAPLYVTELAARSDVWKAPLAPPARNIRPRVLEHEAVTELESLAGFAVAQGNPDKYL
                     AYVTDFDSTTPWKRLTKPAADGRFEPLRAKVGVEVGVNNGPQPYKAASFSPISLRAQS
                     LANVALAPSQPSSGDVLMAWGGVDNGRAQVFATLIGKGGVKRAQRMLTHGTADVADIA
                     LAFVGDGWVVAWVDERSSDPEVYAQKLDSKLNLVGTEQRITRVPGAALDLALQFDGKS
                     LLLAWSDAQASPESGDADIYVQRLRPKDAAPDGSALRVSETPAHSYGPSLAATPSGFS
                     VAWIERGREGERSSVALAELNADFTVERSTAPTIPGDPRALALDCSSVGSCHLVILAE
                     VEDGAVLYGAIRTGKAFSKIEPLIGLDGSALTSVRPVLSGDNLFFADRAETGTRIRRA
                     HLEW"
     gene            complement(9869..13915)
                     /locus_tag="VFQ61_25360"
     CDS             complement(9869..13915)
                     /locus_tag="VFQ61_25360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9957861.1"
                     /translation="MANLKGPFLALVISCAAGVAAWACGSNPELVLVPYEGNGGGANT
                     GGLGGGGRTTVLLPNGGSAGDEDGSPCLTGGCGGPEPSGCGDGTLNEGEQCDDGNGRP
                     GDGCSGICRLEANYTCEVPGEACVPSHRCGDSVVSGAEGCDDGNEQSDDGCSEACEVE
                     PGFTCSEPGALCMPDSDGVAECGNSSVEFGESCDDGARLAGDGCDDECRREPGFTCPE
                     PGSTCTRDEYCGDGRLNGDETCDDGNAEIGDGCNQRCQKESPFYDCSRPGELCASTIV
                     CGDSNVDGDEACDDGNTEAGDGCVADCKMTEPGYVCPHEAGVGGRCIVATAARCGDGK
                     LAPGEMCDDGNVKPSDGCSAVCVVELGYDCPAGKPCTRIEVCGDGKLSTALGEECDDG
                     NVKGADGCSASCKKEAFFTCANPGKACVSQVVCGDGVQINEACDDHNTRNGDGCSSTC
                     KVETGWSCPLGAQCKSAACGDGVVAGNEQCDDANTRGNDGCSASCQLELGFACAPNAK
                     GTSVCRRTVCGDGVREGLEQCDDGNTRPLDACSPTCTNEPSCVDSLGRRKACSPVCGD
                     GIKFPSEACDDGNTRSGDGCSSSCTIERGYQCREVSDATDALTLPIVFRDFTPGAPNG
                     SGHIDFQWNDFAPWGTYLRNPTAQNGINYDYSGLTTGLVRVGLGANADSQGNLSLAGK
                     PVFAYTSPASCPLAPNANGEFWLRRNGVYDCANTVQSAASFAQWFNDGSHATTVPGNL
                     VLLRCPRAGAPANDPVCASGDAGTFLFDSNTMLSNGSVCQGSAACDGFFPLDAQPQTK
                     FSDCAGGSPNGQLNSHDFHFTSEVRYWFQYQAAANATLSFRGDDDVFVFVNGRLVVDL
                     GGIHVAEPGSVTLNAATVDRANQRLNLVENSIYEIIVFQAERNTCASNYRLQLKNFFF
                     SRSQCAPVCGDGVVTPDEACDEGARNVDASGDTYGRCTKQCTLGPRCGDGVLRKDKEA
                     CDNGLNQDTYATASGACAPGCALPPRCGDGAVNGSGGEACDDGAKNSNAYSASGRVCT
                     TSCTLGRFCGDGLVSDGETCDEGLAKNGTASSRCSVACTLRCGNATLDPGEDCDWGAA
                     KNLGGYGGCTAACQFGPRCGDGIKNGGEQCDDGRNDGTYGTCAPECKFGARCGDGVVQ
                     AGAGEACDAGADNVSNGYGEGQCTTRCTPTPYCGDRQVDIDHGETCDDGANTGEPGSC
                     TPDCTAWVALTSCGNGKLDAGEQCDEGRRVNGSAQSGCDRACHLKCGNGIKEAEEECD
                     DGVDDGSYGGCNPDCTLAEYCGDGALNGREQCDAGRENGALRYGPDQCTSTCRVAPYC
                     GDGRIQSEFGELCDSTGGCGSDCQPIPR"
     gene            14237..16012
                     /locus_tag="VFQ61_25365"
     CDS             14237..16012
                     /locus_tag="VFQ61_25365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9957862.1"
                     /translation="MATLTLGQRLLLATGLLSVAATTTLGYGLRTAFRRTEEELFANE
                     FSTLLKPLSQQLEGELSGLGGELRRHCQDPLLDQALLGLSAGDLDSRRVSIVVRVPEF
                     AKALQADELWLVTHRGEVIGAHDTALAGQRSDDLVKRLQAASRGTRLVASGASAGAGL
                     SADVSAQPGAPHSASSQPKPLTSTKPRSGRVEAGCIRSEPGHPERWVGLLAVRSVEQV
                     LARIGNAYGLELALRPATAASEPGAAGAMTRHIELSAFPGSEVQVRRSRVRLTDLLGE
                     LDTEILAIGGATLCLALLGAVLLSRGLARPLVELSRQASERMRGEPRPIEATGPREVE
                     VAAAAFNQAIEDLVTLRRRLAASERIAAWREIARSVAHEIKNPLVPIRAAIETLRRLR
                     ARGDAAFDEYFDEATATALAEVNRIARIVSEFSEFARLPAPKLAPVDPIELTRSVVSL
                     HKATGPSIEFLASPCPSVTADRDQIVQVLTNLVQNALDAVKDVPSPKVIVEVAPRAQD
                     RVRIRVCDNGTGISAEMQARLFVPYATTKPNGTGLGLAIAQRLVVEHGGEIGFEAAPG
                     GGTQFWFDLPTSSGTPPRDTDAPAI"
     gene            16112..17713
                     /locus_tag="VFQ61_25370"
     CDS             16112..17713
                     /locus_tag="VFQ61_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9957863.1"
                     /translation="MTRLTPRQLLALLALLGITFAMWMALGFEQRLGSAFLVLFIAGA
                     AASLLSSADGMPVEALSTFRTQIRRMSDGKAPEMPGSVPTPLIPIYEALIDLHESRAQ
                     VSSPELGEAAQNASSVARVLTDGVAAQMTASDESARVMKEVATAHREIATSVETLAAT
                     AEESSSSVLEMNATTDEVTENIGELASGVRETVASIEEMAYSIREVAKNVDALSLTAE
                     ETSSSMNEMDVSIDQVQSNANETARLSEEVAQDAERGAEAILKTIGEIYRIKESSQEA
                     VAVISNLGSRIDAIGQILNVIDDVAEQTNLLALNAAIIAAQAGEHGKGFAVVADEIKD
                     LAERAGASTKEIADLIKTIQTESRNAISAVERGAVNVDRGVEVSNEAERALKKILESS
                     QKSTNMVRAIARATVEQAKGSKQVTDAIGRIAATVQQIAAATAQQARGSELIMKSGEK
                     MRTIAQQVERSTQEQTNGSRQIKNSIENINAMVNQLNAAHRSLNRGSERALAAATQIE
                     DAARRQDNALHELVTHIDRIKRLGS"
     gene            17710..18006
                     /locus_tag="VFQ61_25375"
     CDS             17710..18006
                     /locus_tag="VFQ61_25375"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957864.1"
                     /translation="MNAGTRGSGSVEPPRAGTMWRRGIAVAIAGVASGCVLGVKGEPE
                     HSATQRPPPRDEQPGPKPPQCRESAANCRYSPGYYHWTPRGYVWIDGHWESTPP"
     gene            18027..19694
                     /locus_tag="VFQ61_25380"
     CDS             18027..19694
                     /locus_tag="VFQ61_25380"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HET9957865.1"
                     /translation="MTPAGPRGPSASAARPQQKRRPWRQFTRAGLALAAPLLLSLWVR
                     AEGPLLHEYVPKDAVEDLELNATTPDGRMPVAIQTPNGPAYAPNLLDPHAPRQVVYGD
                     NSTPDSIDATFRLDSDTSEPSFVHYDDPFTPSVAPFKRLFAFDAVDADFQLGVRDSKA
                     LRPVPLMPEAPPDHDRFFGDLFVDVAPTVLTRIPTVAPGTRLLALRVEPETAITLRQD
                     AAENWFVTSPERKRLRLIFELSAPRAAFGGPFPDVSYEAISHSVTPLPNNVRRAVDDV
                     LARLGLSRNVRPRDAIQTLVSYFRSFAPSDAPPNARGGAALYTELSLEKKGVCRHRAF
                     SFMLTALGLGIPARFVRNEAHAWVEVGDGKTFRRIDLGGAAQRFDIDTPPGAPQHRMP
                     EDPYAWPEGARSALQSTESSPPPNANPRPAASTPRPASSSSATSPVTSSARPAPSFSA
                     PSPAATPPEGQDSPSQIEFAIEPGDVQRGATVRVRGRVTQNESPCPSTRVDIVLSDGA
                     KDNLIGSVPTDSGGQFDSRVTIPFDVELGDHVVRATTPGALRCNPSR"
     gene            19934..21067
                     /locus_tag="VFQ61_25385"
     CDS             19934..21067
                     /locus_tag="VFQ61_25385"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HET9957866.1"
                     /translation="MSKTLEAQYQALPEQLKATLTRYGFDLAQFLELASRVKAGKIED
                     NYVKGELQPPKPEDVQSLPAKDTPAYAELVALGEEELRAGRVALAVLAGGMATRMGGV
                     VKALVEALPGKTFLDLRLQEIESNRQKYGKAPPLWLMASYSTEGPLRQALGARVDHDK
                     IGLFTQFISLRVTPEGELFLDAQGKPSEHAKGHGDFPEALQRSGLLQRFVDGGGRVVM
                     LTNLDNLGGTLDPAIIGWHVKHGQPVTMEVVDKLPSDRGGIPIYVDNVLSMLEEFRIP
                     PSFDPATVRVFNTNVFHFDARALNELKMNWTYFQVNKKVDSKPVVQFERLVNEIAGKL
                     PTRYLLVPREGVESRFLPVKDNEELVQRRAENEAVARARGMIA"
     gene            21064..23592
                     /gene="sppA"
                     /locus_tag="VFQ61_25390"
     CDS             21064..23592
                     /gene="sppA"
                     /locus_tag="VFQ61_25390"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET9957867.1"
                     /translation="MRTLRFGLIGLSLGLGSLLPSVALAQSSVARPLPLPSYGRSVAG
                     TDDGTAVVINPGNLGFMPGPEVRWMGRFLDENALLPYQGHAFSLATSLPFGLGTALRL
                     DLVDPPAALASLGSPFAANYNWLHFGLGYRASDSFGLGFSYKHSFSDNRLTNGIDSWS
                     FGASIRPARRFGISLVAHDVGNPHARSGDALAASYDLALAVRPFATRVLELGLESRYV
                     AEEHGYFVPRATLGVDIPELGRLRGEFSVADPTEEVQTRRWLASAVMSFGYNAVGGAM
                     EFGAGTVFGDALGRSARGQAEENLAFDVAFKGYREPRASEPPMHAIRLRLENTPDNRG
                     HVALLRRLWNIAEQERSAAAVLLELRASPGNSLPHIQELRDAIHKLRTAGKQVFCHLE
                     DADGGALYLCSAANRIWMSPGGGYRFAGLKARYLYFKNLLDNLGVRADFVRIGAHKSA
                     PESFMQEGSSDVARQDKIDLLQQYERQFTLGVASGRNIEPLELRRRIAQGPFIASEAK
                     AQGLVDELAFDDEVDALITKTLGGRVPVIDDNRHAAYAPSSFGSTRGLCIVYVDGDMV
                     DGRSQVIPLLGMRLVGSYTIADSIKKARENPMVGAVVLRVESGGGSALAADVIWREVQ
                     LTRQVKPVIVSMGSSAASAAYYIAAPATRVIANPLSLTGSIGVFYGKADVSELLRKIG
                     VNVELYKTAPRADAESLFRPFTAEEKKELERKVAQFYEQFLSRVSAGRRMTRDQVDAV
                     GQGRVWTGEQALSRGLVDQLGGLRQAIESARELAGLGEEAPIVELPPVETSLLGKLLG
                     IEGLHAESVVLPPELLRLGQALAPFMVHPAEKPLYRMELTDIAP"
     gene            23594..24523
                     /locus_tag="VFQ61_25395"
     CDS             23594..24523
                     /locus_tag="VFQ61_25395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957868.1"
                     /translation="MQAQRAEAVLLELREARWQFASSAQSNELSVRAVGARVALIGAW
                     DVLFRGLAGEARLSGGSASIYGQPPEVALRQGLVGVQRFTLRAPADITPEQYLFESAS
                     LLGRGSSYARREAARAVDEYGLSSVKRYPFSALPVLEARLLALAQATLGEPRALFCEL
                     PLHRFGEAAQRIMLSALERAATGRSLLFSVAQRVYSGPQAELLSRADQRFALERGQLS
                     EPSASPAPPNGEAWLITVSEHPDRFTTRLVTDKIGFEELGPVEAMETLLQGGRSTPVR
                     RFRVWLEGAPARERLLHAARDTEAAILELRPET"
     gene            complement(24555..25739)
                     /locus_tag="VFQ61_25400"
     CDS             complement(24555..25739)
                     /locus_tag="VFQ61_25400"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9957869.1"
                     /translation="MRYRRFGKTELEMPVLTCGGMRFQHSWSDLGLGEIPRDSQTNLE
                     ATIRRALELGMNHIETARGYGTSELQLGQVLPSFPRSELLVQTKVGLTAQTSEFVANF
                     EQSLRNLRLERVELLSLHGINNRQKLDWALRKGGCLEAALRLKEQGLAKHVGFSTHAT
                     TDLILEAIESGGFEYVNLHFYWVNLLNWAAVQAAHRRDMGVFIISPNDKGGKLYTPPA
                     KLVELCRPLTPMQFNDLYCLSHPEVHTLSIGAARPSDFDEHVAALEHYDRIEQTIAPI
                     RARVEQEMWRVLGRDVCERYAEGIPEYVDVPGEVNVREIVRLWVYSKSLDLVDWGKMR
                     YNLLGRGDDWFPGETAGKLDEPALRRVLANSYLKPDFVISALKEAHATLLDAPRKRLS
                     QS"
     gene            complement(25967..26440)
                     /locus_tag="VFQ61_25405"
     CDS             complement(25967..26440)
                     /locus_tag="VFQ61_25405"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET9957870.1"
                     /translation="MAKTIEVGDTAPDFELSDQSGQRQTLRGLCEQGPLVLYFYPKDD
                     TPVCTQEACYFRDDRSAFQEAGAQVVGVSDDSVQSHDKFAQRHGLEFKLLADVGGRVR
                     EAYGVGKTLGLLKGRVTFVIDRERVVRHVFRSQLAARRHVDEALESVRAIAARRA"
     gene            complement(26643..27311)
                     /locus_tag="VFQ61_25410"
     CDS             complement(26643..27311)
                     /locus_tag="VFQ61_25410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957871.1"
                     /translation="MSTWRKPGNLRGMRAARVTVSILVLVPCCVLAACGGRTLDAGFF
                     AETGGVGAGGAVGSGGLAQGGVRPQGGQRATGGRENTGGRAADGGQAPEAGAPSGGVG
                     GEAFGGSGGEPGGKGGNTGTGGRPGTLDRAVKACSSACASIIDACGTAVKSPMETCNA
                     GCSVPLLQATDECLQTSEFLFACVDGVMRKQEGLTCEQAFERVAQDCAIFLLASAGCL
                     PLPQ"
     gene            complement(27366..29051)
                     /locus_tag="VFQ61_25415"
     CDS             complement(27366..29051)
                     /locus_tag="VFQ61_25415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957872.1"
                     /translation="MCVLALAGCSLEKGFRDTSDALSPEDTLYIDGPGYQLLPGKFYG
                     LDYQADLMFARRSDAEASDLMVVDPQRQKMCELPGAVNYRHWVPPWIAYQSEADENGF
                     GRLRFATPQCEPLNFELSSARLPFDITEKGEAVIRVGDGIVRADPLTSEQTSIVEKFE
                     GIRLLSFRASDKVPNYLWLLQIDRSLTAFDADWTQLGTYGREIRRLVGLSDSSLLLED
                     ADGLSILSASAGPHLSLTPFATDACEATGGKWITYFSPCEERRLKTRSASRGPLEFGV
                     SVHPSLTKFVSLPDDKRPGQFIDQLYYLQDIDPNTGLGSLYVTEPLPEGSTAEADGID
                     APEPRLLAEQADLGWLSVQSEGVYVLAKVRDGIGTLMFIDREFAEHKLVTGVPRQIPN
                     ASNYGVDGFVLSNADVATGTGELVQVDRIPSGSESPVPAVNFAAAKVPLDGGFIADTG
                     SGSAVLQDFDGARGTLAMASGPRIGQSAGSGVPKWGFVSLTGFGLPGVAFFSQYNFEK
                     GTGNLEYWNVEIDARGIVSNNVSEFMLVPWPHAGVVYAVQNGDQSGVWFARAK"
     gene            29347..>29687
                     /locus_tag="VFQ61_25420"
     CDS             29347..>29687
                     /locus_tag="VFQ61_25420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9957873.1"
                     /translation="MSAEVAMCSAAWAQPGTPAADSATRAPESEFSAPEPLQASVEYP
                     ADASGRAEVLLELTIDPEGHPRDVAIVEGAPPFAEAAATAAAQWQFKPARLRGRNVAA
                     RIRYTVRFEPEP"
BASE COUNT         5215 a        10048 c         9176 g         5248 t
ORIGIN      
        1 ccgatgctcg cgaacaagac gcgcaggtaa tcgtgaactt cggtgacggt gccgacggtc
       61 gagcgcgggt tactggaggc gctcttttgc tcgatgctga tggtcgggga gagcccgcgg
      121 atcatgtcgt acttgggctt ctccatctgg cccaagaact gccgtgcgta gctggagagg
      181 ctctccacgt agcggcgctg cccctcggcg tagagcgtgt cgaacgcgag tgaggactta
      241 ccactgccgg agacgcccgt cagaatgacg agccgatgtt tcgggatgcg gagctctacg
      301 ttcttgaggt tgtgctcacg tgctccgcgc acgtaaatcg aatccgacag gtcactcttc
      361 atgcgaatcg agtcctagcg cgtgcgaaat cgagcgcgcg caatggtagc tcagtcttcg
      421 gcaatggcag gcctatccac gcctcaccgc gcacccgccg taccaacggt gacaatcgag
      481 gacactggca cgctcgagtt ggacgatcga gctaggcgcc tggctgggct cggttttggt
      541 gcccggcgtt cggtttcgcc tcgtgctcag cccttcactg gaccaagccg cgaatgttca
      601 ctccgccggc ggcgcttccg gttcttccgg attgcgagct gcggctttgc gtgcgcggaa
      661 caacagcgag tccatggacg cggccgacaa gtgcgcggct agtgccgctt cgttcacggg
      721 cgcgtagctc gcgtgcacgt ccacctcctc gacctccacg actgcgttcg ggttcggcac
      781 gtcctcgaag ccgagtacat ggtcgaacaa ggcgtcggcg ttcgcgaact ctggaggcaa
      841 aagatctaca ttgcctgact tacgcagccc atcgaccccg aagcgcgcgg cccatcgcag
      901 cgtctcttgg acgccagcgc ccgtgcacgc ggccgcggat acgagcggga cctcggccgc
      961 gagcccaagc cttgccgcga tttcctcgag cgaagccgcc ccaggcgtat cctgcttgtt
     1021 ggcctgcacg acgagcggca cgggcgtctc tcgctgcttg atagagctgc gcaggcgcgc
     1081 aaagcagcgc agactgtcgg gcatcttctg aacgctggaa tcacagacga acaccagcac
     1141 atccgccatc tcgaccaggg gcttacgtcg gtagttgcgt tcgatctgcc ctggcacgct
     1201 gatcagctgg aacttcagcg aagtttcgaa atgcttcggg ccgtccacct cgagccaatc
     1261 gaagaacatg gtgcggcctt tgagcgccgc cggcgtgtac atttcgctcc gcctcgtggc
     1321 cggcaccatc tgagagattc gctcgagatt ggtggttttt cccgcgtaac cgggaccgtc
     1381 gtagaccacc ctgattacgt agcatccgcg agacgcatcg tacgacgcca tgcccgagct
     1441 tagcgcccga tggagattgg tctagccggt cttttatata tctggagccg agtccggccc
     1501 gcggggctgc gcgctcacta ccagtgaacg cgcacgccgc tcatcgagcc gccgctagtc
     1561 gaggctgact ccttgtcgcg agcgcagctc gacataacgt tcgctgaccg agtaggccat
     1621 caggttttcc agacgagcct ccacgctcgg gctgccttgg gcggagggca ccgattggat
     1681 cagggcaccg acggtttcga ggtcgtgaca cacggccaat ccagcgcgat cagcgctgaa
     1741 atccacggcg tggacccagc gcttcaggtc caacgcagtc tcggacgaga gcagcttggt
     1801 caccacgtga gtcaattgtt cgatgcccgc tccagtggcc aagcgcgcta gcaccttgct
     1861 ggaatccgcg acgggcgcct cgaggtccgg cgtgaccggg aagcgcggct tcaccaatct
     1921 aatcgcggca aacagccaag ccttcagtgc agtcaggttc ggcaagagct gcctcaaata
     1981 gaggcctggc tggtaatacg cgatgtgatg gcccgcgatg aacgcggctt ggagcgactc
     2041 gaagttggcg ttgaaagccc cgccgcccac cagaatcgca gggggcagtg tgggcaaaaa
     2101 cccgagcacc ccagggtctt ccggccgctg atacatgggt ggctcgggaa tgcccagagc
     2161 ttccgctgcg taatgcgtcg ccagcacgag cccgtacggg tagctctccg cgtcgatccg
     2221 gtggtcttcg gcaagcccaa acgcctccag cggctccgct cgcgctttga ccacgaacgg
     2281 ctcgaccaag gcaaagagct gcgtcagcag cggctccgca ccctcgggcg cgatgagctc
     2341 gttccactcc ggcgccgaca gcgagcgttg aatggccgga ggatccgtgt cgcgcatgcg
     2401 tgagaagaaa cgagactcgt cgggctcggc ttgacccagc acgtgaagcg cttggcatgc
     2461 caggaatgcc ccgtccgcgc ggcgcgcctc ggtgtacacc ttgcgcagca tcttgtatgg
     2521 cgtcggctgg tagggatcgc gctttaccca agccgtcagg ctgccgaccg cttgctcgaa
     2581 gtactgcgtg ggttccgccg agtagagctg tgagatcttc tcctggtacc gcggattgtc
     2641 acggtccagc tccaacatcg attcgtagac gcgcaccgcc tggtcgtggc gttgcaagtg
     2701 ctgctcgtag atttccgcga gctgctcgag cacgcccagc aaatcacctt tgtcttcgcg
     2761 cggcgcgagg tcttgcgcgc gacgcttcaa catctccttg agcccctcgt agtcgcgcac
     2821 ttgccgccgg agcttcaatc cctcgttgaa cgcgggctcg ttttcgggat cggactcgag
     2881 cgccaaatcg acgaactcga tggcgcgcgt cggatcgcgg agctcgcgcg cggcgagcat
     2941 ggccgccgta tgcaagtact tcgcgcgctg cttcgggtcg tccacgacct ccgccaagcg
     3001 cgagatcacc tcgatgagct gaggccaatc cttctcggcg ctgtacagct gcatcagctt
     3061 ggccaagatc ttgcggtcgt tcgggcgctc gtcgagcgcg agcaacagag cgcgcgccgc
     3121 atagtcgggg ttgttcagct tggaggccgc gatgtcgccg atctcgaaca acgtttgcac
     3181 gcgctcatcg ccttgcacga gctcgagctc ccggtagcgc gtttcgatca cgcgctccca
     3241 ctcgtcgcgg gcggcgtaca ccttaccgag agcgcggaag ctggcggcgt cgctcgagtc
     3301 agcgcggatg gcgcgctcca gggcctcgac cgcctcacgt tcgcgtccga gcttgaacag
     3361 cgcttcgccg cgcctacggt acacggcccc gagctcgctg ccgccgagct ccgagtcgtg
     3421 ccgttcgagt agcaaatcgc agaccgcaag cgcgcgttcg gggctgccgt gggcagccgt
     3481 aagctccgag accgccaaca gcgcctgcgc gtctttggcc aatagctcga gcaaatcgcc
     3541 gaccgccgcc accgccttct cggtcatgcc ggactccgcg agcgagtcga tgtacacgaa
     3601 cgcggtctcg gccgcctcct cggcgtccaa cgtgacgagc cccggaatcg agggctcgag
     3661 tcgttttgcg gcttcgacat agcgttcttc cgcatacgcg agccgcccct gcacgcgcga
     3721 ggcttccggg ttggtagggt ctaggtgcaa ggcgatttga gcggccgcgc tggcgcgctt
     3781 gtcgtctttg aggtggcgct ggcacagcac ggcgaggtcg cccgcgagct tggcgcgcgc
     3841 gtgcgatcct tcggtacgtt cgagctcctc ttcgatgagc tgcacggcag ccgcgtagtt
     3901 cttgtgctcg aggtacttct ctcgcacttt gcgcgtgatc gccgcgttgg ttggacaagc
     3961 atcggctgcg agtttgtatc gcagcagcgc gcccgtgttg tcgccttgtt cctcgagcag
     4021 cgcggccgct ctcaggtact gctcggcacg cgcttcaggg gtcgtcgcac gctcggcgag
     4081 ctcttcgacc gcacgcactg cgctctcccg atcaccggag gcggcgcgca aattcgccaa
     4141 agcttcgagc gcccccgcgt ggctcggcgc gagctcgagc acccgctcgt aagccgcgat
     4201 ggcccgcggc aaatccttga gttgctcacc cagtacgcgc ccgagctgca gcccgaggtc
     4261 cacccggcgc gcgcttccgc tctccaaaga gagctgcttc tcgtacaagc ccgcgagatc
     4321 ctcgaagcgc tcgaggacgc gcaggtgacg cgcgagatcc gtggctgcgc tctcacggct
     4381 cggatcgagc tctagaattg cttccagcgc gcgagccgcc tgcgcatgat ccaaaaactc
     4441 ttcgtcgtag agcttggcga tccgctcgtg cagggcgatc ttctgacggg ccgttaccgc
     4501 tagctcgagc tccgcctcga gcgtctcgat caggtcttcg tagcgagcgg cccgggtgag
     4561 cagacggtcg atgcccttca gcgcatccag gtgcttcggg ttgtccttga ggatctggcg
     4621 ataggcgcgc tcggcgtcgg ccaacttgtc gagctcgtgc tcgtaggtct ccgcgatttc
     4681 gagcagcgat ttgtgccgct cttccccgga aagaacctcg gcccgcgcct cgagcacgcg
     4741 cagtgcgcgc gccgtgtccc cctgcgcttt gagcagccgc gagaagccct cggccgcgcg
     4801 cgcgtgcccg ggatcttcgg ccaaaatgct ctcgtaagcg tcctgggcgg cctgcatgtt
     4861 gctgagctgg ttgaccagca aatccgcggc ttcggcccgt aaatcccggg cctgattggg
     4921 cggcgcgatg tcggcccgcc gcatcaaggt ttgcccgagc tcggaccact gttgcgcctt
     4981 gcggtacagc tgcgccaact ccgcgagggc acgatcgtga gcgggctcga gctcgaccga
     5041 tcgcgtgagc cacggcaagg ccaagtccgg tcgggacact ttctccgagt accaacgccc
     5101 aatctggaac gcgagggcgg ccttggcgtc gtccgccagg tcactctcca tggcttccag
     5161 acagtgactg aagacttcct cccagtccgg gtagcgggag cccgccagtt tctcgacttg
     5221 ctcggagagc ttctcgttac cggggtcgaa acagaacgct tgagtggcag cgaccagcgc
     5281 ctgttccgga tctttgacct cgttgctgta gatgcgcgcg atctcggcga aatatcgggc
     5341 acgctccgtc gcgaccttgg ccgcgtcgct gcgttcgatc agggactcga tcagctcgtc
     5401 gaacttgccg aggcgcttgc gcaagcgatc gagtgactcg accacctgcg tatcctcggg
     5461 gtctagtttg accagcccct gatacagctt ctctgctgcc tcgtggcgat cggccatctc
     5521 atagagccgc gccgcgcgcg ccaggagctt gcgcacgatg cggtcactgc cgacggtgtt
     5581 cttggcgcgg acctcgcgag ccacactata gaagccgtcg gccagacgca gcggcggaac
     5641 gcccaaatcg accagggctt ctacctccga agccttctgt tcgtcccaag ccacgtccgt
     5701 gagcgcctgg aaccgctcct ccaccaactc gtccgcgacc gagctcggtg ggcgttggga
     5761 ggcgcgccac agccgccgga gcccctcttc cacctcggtg gcgtcacgcg gacggagagc
     5821 acgatccttc tccatcagca gcttcacgaa cacgtccacc tcgggggtag cgccgttgcc
     5881 cgccgcggcg aatgacagag actccgcgtc tttggtgaga tgcccgagcg caacgtcgac
     5941 cggtgaactc ccgacgaagg gtgctcgccc cgacatcgtt tgataaatta gcgtgccgag
     6001 cgcatacacg tcggtgcgcg cgtccaaggc atcgccccgc aacagctcgg gggaggccga
     6061 agccagccag ttcgcgctcc cgctacgcgc caagcccaac aaaccgggcc gcaagtggtg
     6121 cgcgcccgca tccagcagca cgatctcagg gcgtgagttt gcgccctcga gcaccaacac
     6181 gttgcccgcg tgcaaagcgc catgcaccaa gccttgcgcg tgcaccgcct tcagagcgtc
     6241 gagcaaagcc cagaacagcg gcaagagttc gttgatatga cgcccgccgc ggtcgctgat
     6301 ccatttggcg agcggctcgc cttcgcggaa cgtgtgcgcg acgccgaaga gccgcccgct
     6361 atcctccagt gctcccgccg cgaccagact cggaagcccc tcatgggtag cattgctcgc
     6421 tacgcgggtc gccgcgaggt aacgctgcac gtcgcgtcgg cgggtacgaa gctcgcgcga
     6481 caagcgacga accctcaagt cctccgcgtt tttccgcgcg cgatagacgc tgcccgacgc
     6541 gctcgtgccg agctctccta cgatcaggta ttcgccgagt tcatcccccg gttgaggacg
     6601 ctccgaggcg tcgatcgcgc cgttgcgtag tttcttgacg agctcctgag gcaactcgcg
     6661 acgtgacgcc agcaccacgt cgatcagggc gtcgacagcc tccacttcga cacatttctg
     6721 agacagggcg cgggcgaacg agcccttggc gctcgcacca cccacctcgc tcggatccat
     6781 gccgagcaag ctcgagcaaa gagcctttag ttcttccagc tcatacagcc gttcgagctc
     6841 tgcgctcagt gattcgacga ccatcttcgt gctcctcggc tcgactcatt tgggggaccg
     6901 gcagcaggct gccacgcccg tcgggagcca ggcttaccag tgtctgcgag ccggttccag
     6961 tcagagcgaa cggtcgatgt cggataacag ggcagattgt ggcttttaac gacatcttcc
     7021 cacctaattc cagttttgct taaccagagt caggcggcgc gccgccgttc agaggtgccg
     7081 ctgccgaccg ccctcgcgac cggatcacca ctcgaggtgc gcgcggcgga tgcgagttcc
     7141 ggtctcggcg cggtccgcga agaacaggtt gtctccactc aagacgggac gaacggaggt
     7201 gagcgccgag ccgtccaagc caatcagcgg ctcgattttc gagaacgctt tgcccgtgcg
     7261 gatcgctccg tagagtaccg caccgtcttc gacctccgcc aagatcacca gatggcaact
     7321 gccgacggag gaacaatcga gagccagcgc gcgcgggtcc cccggaatcg tgggcgcggt
     7381 cgagcgctcc accgtgaaat ccgcgttcaa ctccgcgagc gccacgctgc tccgctctcc
     7441 ttcgcgcccg cgctcgatcc aagccacgga gaacccgctc ggggtcgcgg ccaagctcgg
     7501 tccgtacgaa tgagcaggcg tctcgctcac gcgtagcgcg gagccatccg gtgctgcgtc
     7561 cttggggcgt aaacgctgca cgtaaatgtc ggcatctcca ctctccggcg aagcttgggc
     7621 gtccgaccaa gccagcagca ggctcttgcc atcgaattgc agagctagat cgagcgcggc
     7681 ccccggaact cgcgtgatgc gttgctcggt gccgaccagg ttcagcttgc tgtcgagctt
     7741 ctgcgcgtag acctcgggat cggagctgcg ttcgtcgacc cacgccacca cccaaccgtc
     7801 gccgacgaac gcgagggcga tatcggccac gtcggctgtg ccatgagtca acatgcgttg
     7861 ggcacgcttc acgccgccct tgccgatcaa agtcgcaaac acctgagctc gcccgttgtc
     7921 cactcctccc caggccatca gcacgtcccc actgcttggc tgcgatggag ccagcgcaac
     7981 attggccaga gactgcgcgc gcaatgaaat gggcgagaag ctcgccgcct tgtacggctg
     8041 cgggccattg ttgaccccta cttctacgcc gaccttggcg cgcagcggtt cgaagcgccc
     8101 gtccgcagcc ggtttggtga ggcgcttcca aggagtggtc gagtcgaagt cggtgacata
     8161 ggcgaggtac ttgtcagggt taccctgtgc gaccgcaaaa cctgccagcg actcgagctc
     8221 ggtgaccgcc tcgtgctcca acacccgggg gcgaatattg cgcgccggtg gcgcgagtgg
     8281 ggctttccac acgtccgagc gcgccgcgag ttcggtcaca tacaggggcg ccggcacgcg
     8341 ggtcagcgcg gccacgccga agcaacccga gccggtgcat gcgaggccta cggccaactc
     8401 ggcgacctta ccctgcagag catccagacg cagtggctcg ctcgccagcg gggtcagagc
     8461 cgcatcgaag ctgacgaaca gcggaaccac cggttggtct ccgcactcgc cccgcttcgg
     8521 acagggcttc gtcgacgtga ctaccgccag ccctcccgcg gaggcgacga agtcgggaat
     8581 gccgtcctcg cgatcgtacg cgagttctac ccggcgtccc ttgagccgcc cttcaccgtc
     8641 gagcgcggat agcgagatat ggcgtccggg ttcggtaccg gtgaagcgtt cgaacgcgac
     8701 gtacccttgc ttggcgccgg ggtactcgga gacgagtcgc accaaggcct gctcaccttc
     8761 ttttgcggtg agcggagttg gctcgcgcac gagcttctgg tcgctatcga gcgacgcgat
     8821 cgaaatgtgc gggtccaggc tgcgatgatc ggtgaacgcc accaggaggt gcgaatcgac
     8881 ataggccgcg tcgagatcca gctccacctc ggccccaccg acgaccgtga cggagcgctt
     8941 gatcttgccg tcgttggtga cgctggtgga ccacacactg cggttgttcc ccgtacccgg
     9001 gcgcatggcc gtgagctgcg caccttccgc gaacgcggtg gcctgccaag caagcgcctg
     9061 tccttcgagc aattccgctt caccgaagcg acagctcgcg tctagacgcc gcacgcgaac
     9121 ctccgcggcg cgggcgttgt tgctgcgcgt cgcgtagaag accagagcgc cgcccgcaac
     9181 cggcaccgcc tccacccaca aagcacgtgc gggcagtgac gtcgtcgcgg gcttctgatc
     9241 tttcgcggta aggccttccg cactcgtgca caaagcctcc aaggtctcct cgcgggaggg
     9301 ttcctgtcgc gtgtagagca aggcgtaacc ggagctcaac ggacgcacca agaccacacc
     9361 catttccgcg ggagctggcg cgagcagttg cgcggccttc agcgggcgcc ccgtcgctga
     9421 caaggccacg gactggaagc gccgttcatt gccttcgttg gccgcccaca cgaccaggcc
     9481 gccgtcagct cgagccgcta aataaggacc gtaagttgca ccgggaaccc gaccaatctc
     9541 ggttgcgtgc aagctcggca ctccggggtc gcgcagcggc gccttgggcc gtgcgcgtga
     9601 aggcagggga ggcagtgatt caggaggctt gctgcacgaa gcgaggcaca gcgcggcgcc
     9661 agccatgcac gacaggagga ttctcacggg gcgtgaacgt acgagcgcac tcccgagcat
     9721 gcaagccagc agaccgagac ggacgcagcg ccggggttac tcggggtccg ttccggcgag
     9781 tcgttttcag cctagatttg gaattcacta gctgcggtgc acgagctgcc accgtgaccc
     9841 agcccgaacg gcgcggtccg gagtcggctc agcgcggaat cggttgacag tctgaaccgc
     9901 aacccccggt gctgtcacac agctctccga actcgctctg aatacgtcca tctccgcaat
     9961 aaggagccac gcggcaggtg ctggtgcatt ggtccgggcc gtagcgcaga gctccgttct
    10021 ctcgcccggc atcgcactgc tctcgaccgt tgagggcgcc atctccacag tactcggcga
    10081 gtgtacagtc ggggttgcag ccaccgtagc tgccgtcgtc gacgccgtcg tcacactcct
    10141 cttcggcttc ttttatcccg ttgccgcatt tgaggtggca agcgcggtcg cagccgctct
    10201 gggcggagcc attcacgcgc cggccttcgt cgcactgctc tcccgcgtcc agcttgccgt
    10261 tgccgcacga agtcaacgcg acccaagccg tgcagtcggg cgtgcaggag ccgggctccc
    10321 ccgtgttcgc tccatcatcg cacgtctccc cgtgatcgat gtctacttga cggtctccgc
    10381 agtacggtgt cggcgtgcaa cgcgtagtgc actgcccttc gccatatccg ttgctgacgt
    10441 tgtctgcccc cgcgtcgcaa gcttctccgg ccccggcctg tacgacgcca tcgccgcagc
    10501 gcgccccgaa cttgcattcc ggcgcgcacg ttccgtacgt gccgtcgttg cggccatcgt
    10561 cacactgttc gccgccgttc ttgatgccgt ctccgcagcg cggaccgaat tggcacgcag
    10621 ccgtgcaacc gccatagccg ccgagattct tggccgcccc ccaatcgcag tcttccccgg
    10681 gatccagggt ggcgttgcca caacgcaacg tgcaagccac gctgcaacgg ctgctggcgg
    10741 taccattctt cgcgagacct tcgtcacaag tctcgccatc gctcactagc ccatccccac
    10801 agaagcgccc gagcgtgcac gacgtcgtgc atacgcgacc actcgcggaa taagcattgc
    10861 tgttcttcgc gccatcatcg caagcctcgc cgcccgagcc gttcaccgcg ccgtcgccac
    10921 agcgcggcgg cagcgcgcaa cccggcgcgc acgctccgct cgcggtggcg taggtatcct
    10981 gattcaagcc gttgtcacag gcttctttgt ctttgcgcag cacgccgtca ccgcagcgcg
    11041 gcccgagtgt gcattgcttg gtgcagcggc cgtaagtgtc tccggacgcg tccacgttgc
    11101 gcgcgccctc gtcgcaagcc tcgtcgggag tgaccacgcc gtcgccgcac accggagcgc
    11161 actgcgagcg actgaaaaag aagttcttca gctgcagacg atagttggag gcgcacgtgt
    11221 tgcgttcggc ttgaaagacg atgatctcgt agatgctgtt ctcgaccagg ttgaggcgtt
    11281 ggttggcgcg atccacggtc gccgcgttca gggtgacgga gccgggctcg gccacgtgaa
    11341 tgccgccgag atccacgacc aagcgtccgt tgacgaatac gaacacgtcg tcatcccccc
    11401 ggaagctcaa ggtcgcgttg gccgcggctt ggtattggaa ccagtaccgg acctcactcg
    11461 taaaatgaaa gtcgtggcta ttgagttgcc cgttcggcga cccgcccgcg caatcgctga
    11521 acttggtttg gggctgggca tcgagcggaa agaatccgtc gcacgccgcg ctgccttggc
    11581 acacgctgcc attgctcagc atcgtgttgc tatcgaacaa gaacgtccct gcatcgccgc
    11641 tcgcacacac cggatcgttc gccggcgcgc cggcgcgcgg gcagcgcagc aagaccagat
    11701 tcccgggcac ggtggttgcg tgactgccgt cattgaacca ctgcgcgaag ctcgcggcgc
    11761 tctgcaccgt attggcgcaa tcgtacacac cgttgcggcg caaccaaaac tcaccgttgg
    11821 cgttcggagc gagagggcag ctggcggggc tcgtgtacgc gaacactggc ttgcccgcca
    11881 agctcagatt gccttgtgag tcggcgttgg ctccgagccc aacccgcacg aggccggtgg
    11941 tgagcccgct atagtcgtag ttgatgccgt tctgggccgt ggggttgcgc aagtaggtgc
    12001 cccacggcgc aaagtcattc cattgaaagt cgatgtggcc gctgccattc ggcgcgccgg
    12061 gcgtgaaatc ccgaaaaacg atcggcagag tcagagcgtc ggtggcgtcc gatacttctc
    12121 ggcactggta gccccgctcg atcgtgcagg acgagctaca accgtcaccc gagcgggtgt
    12181 tcccatcgtc acatgcttcc gacgggaact tgatcccatc cccacacacg ggggaacaag
    12241 ctttgcggcg cccgagcgag tccacgcacg aaggctcatt ggtgcaggtc ggtgagcacg
    12301 cgtccagagg ccgcgtgtta ccgtcgtcgc actgctcgag cccctcacgc accccgtcac
    12361 cgcaaacggt gcgtcggcac acgctggtgc ccttggcgtt gggcgcacac gcaaaaccga
    12421 gttcgagctg acacgacgcc gaacacccat catttccgcg ggtattcgcg tcatcgcact
    12481 gctcgttgcc ggccactact ccgtcgccgc acgccgccga tttgcactgc gcgcccagcg
    12541 ggcacgacca acccgtctcc actttgcaag tactcgagca gccgtctccg ttgcgcgtat
    12601 tgtgatcatc gcaggcctcg ttgatctgga ccccatcccc gcaaacgacc tgagacacgc
    12661 aggctttgcc ggggtttgcg caggtgaaaa aggcttcctt cttacaagaa gcgctacacc
    12721 cgtctgctcc cttcacgtta ccatcgtcgc attcttcccc gagcgcggtg ctgagctttc
    12781 cgtccccgca aacttcgatc cgggtgcacg gcttgccagc agggcaatcg tagccaagct
    12841 cgacgacgca cacggccgag catccgtcgc tcggcttcac gttgccgtcg tcgcacattt
    12901 ccccgggcgc aagcttgcca tcaccacatc gagcggcggt cgccacgatg caacgaccac
    12961 ctacgcccgc ctcgtgcggg cagacgtagc cgggctcggt catcttgcag tccgcgacgc
    13021 acccgtcccc ggcttcggtg ttgccgtcgt cacaagcttc gtcaccgtct acgttcgagt
    13081 cgccgcacac gatcgtggag gcgcagagct cacctggcct cgagcagtcg tagaacggag
    13141 attctttttg gcagcgctgg ttgcatccat ccccgatttc cgcgttacca tcgtcgcaag
    13201 tctcgtcgcc gttcaatcgg ccgtcgccgc aatactcgtc gcgagtacac gttgaacctg
    13261 gctctggaca cgtaaaaccc ggctctcgcc ggcactcgtc gtcgcagccg tcccccgcca
    13321 gacgcgcgcc gtcgtcgcaa ctctcgccga actctacgga ggaatttccg cactcggcca
    13381 ctccgtcgct atcgggcatg cagagcgcgc cgggttcact gcacgtaaag ccgggttcca
    13441 cctcgcacgc ttcggagcac ccatcgtcgg actgctcgtt tccgtcgtca cagccttccg
    13501 cgccggagac cacactgtct ccgcagcgat gactcggcac gcaggcttcg cccggcactt
    13561 cgcaggtgta gttcgcctcc aaccggcaga tccccgagca gccgtctccc gggcgcccgt
    13621 taccgtcgtc acattgctcc ccttcgttca gagtgccatc cccgcaaccg gacggctctg
    13681 gtcccccgca tcctcctgtt aggcaggggc taccgtcctc gtcgcccgcg cttcccccgt
    13741 tcggcaaaag aaccgtcgtt cggccaccgc cgccaagccc tcccgtgttc gcgccgccgc
    13801 cattgccttc gtagggcacc aacacgagtt cgggattcga accgcatgcc catgccgcca
    13861 cgccggctgc gcaggagatc acgagcgcca gaaatggacc ttttaggttt gccatgcaga
    13921 ctcgagaagc aacgaaggtt gaccaccggc cgcgagcgca ggagcgagcg cagaatcgag
    13981 ctcgcggcca cgcgaggaag acagacgcaa gccggcgagt gctggcgaat agcctcccag
    14041 agagggcgct tagataacaa ccaccacgag cgacctcgct ctattatgca gaagccccgc
    14101 gcctttgccc gaataacttg cgcgcttcgg agcgggcatg tgtttcaggt attctgcgcc
    14161 gtccacgaat tctctatgaa ctcgagccgt ctttgaggct gggtgtgctg aatagctctc
    14221 ttccaacgaa aacgtcatgg cgactttgac actcgggcag cgcctcttgc tcgcaactgg
    14281 actcctgagc gttgccgcca cgactaccct cgggtatggc ctgcgcaccg cattccgtcg
    14341 caccgaagaa gagctcttcg cgaacgagtt ctccacgcta ctcaagcctt tgtcccagca
    14401 gctggaaggc gagctctccg ggctcggtgg cgagctccgg cggcactgcc aagatccgtt
    14461 gctcgatcag gccttgctgg ggttgtcggc gggggatctc gattcacggc gagtatcgat
    14521 cgtcgtgcgc gtgccggagt tcgccaaggc cctgcaagcc gacgagctct ggctcgtgac
    14581 tcaccgggga gaagtaatcg gcgcacacga caccgcgctc gcgggacagc gctccgatga
    14641 tttggtgaag cgactccagg cggcttcgcg cggcacgcgg ctcgtcgcct ccggagcctc
    14701 agccggagcc ggattatcag ccgatgtctc agcccagccc ggcgcgccgc attccgcctc
    14761 gtcacagccc aagccgctca cctccacgaa gcctaggagc ggccgcgtcg aggcaggctg
    14821 cattcgttcg gagcccggac acccggagcg ttgggtgggg ctgctcgccg tgcgatcggt
    14881 cgagcaggtc ttggctcgga ttggcaatgc ctacggcctc gagcttgcgc tgcgaccggc
    14941 gaccgcagcg agtgaacccg gcgccgcggg ggcgatgacc cgtcacatcg aattgagcgc
    15001 gtttccgggc agcgaggtgc aagtgcgccg ctcacgagtc cgccttacgg atctgctggg
    15061 ggaactcgac accgaaatcc tcgcgatcgg tggggcgacg ttgtgcttgg cgcttttggg
    15121 agcggtgctg ctatcgcgtg gtctagctcg tccgctggtg gagctgtcac gccaagcgag
    15181 tgaacgcatg cgcggtgaac cgcgacccat cgaggcaacg gggccccgcg aagtcgaagt
    15241 ggccgccgca gccttcaatc aggcgatcga ggacctcgta acgttacggc gccgcctcgc
    15301 ggccagcgaa cgcatcgccg cgtggcgcga gatcgcacgc agcgtggccc acgagatcaa
    15361 aaatcccctg gtgccgattc gagcagcgat cgagaccctg cgacgactcc gcgcgcgcgg
    15421 cgacgccgcc ttcgacgaat acttcgacga agccacggcc acggcgctgg cggaagtaaa
    15481 ccgcatcgca cgcatcgtgt ccgagttcag cgagttcgcc cggctgcccg cgccgaaact
    15541 cgccccggtg gatccgatcg agctgacccg ctccgtggtc tctctgcaca aagccacggg
    15601 acctagtatc gagttcctcg cgagcccttg cccgtccgtc actgccgatc gggatcaaat
    15661 cgtgcaggtc ctgaccaacc tggttcaaaa cgcgctcgac gccgtaaaag atgtgccaag
    15721 ccccaaggtc atcgtcgaag tggcaccgcg ggcccaggat cgcgttcgca tccgagtgtg
    15781 cgataacggc accggaatct cagccgagat gcaggcgcgc ctgttcgtac cctatgccac
    15841 cacgaaaccg aacggcaccg gtctcgggct ggcgatcgct cagcgcttgg tcgtcgagca
    15901 cggtggagaa atcgggttcg aagctgcgcc cggcggcgga actcagttct ggttcgatct
    15961 tccgacatcg agtggcactc ccccgcgaga caccgacgcg cccgccatct gacccgccgg
    16021 ccagacggcg caccttcctt caggctgact cgctagaagc ttgttcacgg ggcagtccgc
    16081 caagtacgct gtttttcctt cgggaatatc gatgactcgc ctcacgccgc gccagctctt
    16141 ggcgttgctc gcgctgttag gaatcacgtt cgccatgtgg atggcgttgg gtttcgagca
    16201 gaggttgggc agcgcgttct tggtcctatt catcgcgggt gctgccgcga gcctgctttc
    16261 ttccgcggat ggcatgcccg tggaagcgtt gtcgaccttc cggacacaga tccggcggat
    16321 gagcgacggc aaagcgcccg agatgcccgg ctcggttccg acgccgctga ttccgatcta
    16381 cgaagcgctg atcgatttgc acgagtcgcg tgcacaagtt tcttcgcccg agttggggga
    16441 ggcggcgcag aacgcgagct cggtcgcccg ggtcctcacg gacggggtcg cggcgcaaat
    16501 gacggcgagc gacgagtcgg ctcgggtcat gaaggaagtg gccacggcgc accgggagat
    16561 cgccacgagc gtcgagacgc tggcggcgac ggccgaggaa tcgagctctt cggtgctgga
    16621 aatgaacgcc acgaccgacg aggttaccga gaatatcggc gagcttgcct cgggcgtgcg
    16681 tgagaccgtc gcctcgatcg aggagatggc ttactcgatc cgcgaggtcg ccaagaacgt
    16741 cgatgccctg tcgctcaccg cggaggagac gtcctccagc atgaacgaga tggatgtctc
    16801 gattgaccaa gtccagtcga acgccaacga gaccgctcgc ttgtcggagg aagtggcgca
    16861 agacgccgag cgcggcgcgg aagcgatcct gaagacgatc ggtgagatct accgcatcaa
    16921 agagagcagc caggaagcgg tggcggtgat ctcgaatctg ggttcgcgca tcgacgccat
    16981 cgggcaaatc ttgaacgtga tcgatgacgt cgccgagcag accaacctgc tcgccttgaa
    17041 cgccgccatc atcgcggcgc aggccggtga acacggcaag ggcttcgccg tcgtcgccga
    17101 cgaaatcaag gacttggccg aacgcgctgg cgcgagcacc aaggagatcg cggatctcat
    17161 caagaccatt cagacggaga gccgcaacgc gatctccgcg gtggaacgcg gcgcggtcaa
    17221 cgtcgaccgc ggcgtcgagg tctccaacga agccgagcgc gccttgaaga agatcctcga
    17281 gagctcgcag aagagcacca acatggtgcg cgccatcgcc cgcgcgaccg tcgagcaggc
    17341 caaaggcagc aaacaggtca ccgacgcgat cggacgtatc gcggcaaccg tgcagcagat
    17401 cgcggcggcc acggcgcagc aagcccgcgg ctcggagctg atcatgaaga gcggcgagaa
    17461 gatgcgcacg atcgcgcagc aggtcgagcg ctccactcaa gagcagacca acggcagccg
    17521 ccaaatcaag aactcgatcg agaacatcaa tgccatggtc aaccaactga acgcggccca
    17581 tcgctcgctg aatcgtggct cggagcgtgc gttggcggcc gctacgcaga tcgaagacgc
    17641 agcccgacgt caggacaacg cgctccacga gctggtcacg cacatcgatc ggatcaagcg
    17701 gctcgggtcg tgaacgcagg aacgcgcggt tcgggctcgg tcgagccgcc acgcgccgga
    17761 accatgtggc gccggggaat tgccgtcgcg atcgccggtg tggcctcggg atgcgtgctg
    17821 ggcgtgaaag gcgaacccga acactcggcc acgcagcgcc caccaccacg cgacgaacag
    17881 cccgggccca agccgcccca gtgccgggag agcgcggcga actgccgcta ttctcctggc
    17941 tattaccatt ggacgccccg cggctacgtt tggatagacg ggcactggga gagcactccg
    18001 ccataattcg tggaacactc ctcgcgatga ccccggctgg ccctcgcggc ccgagtgctt
    18061 cagcagcgcg cccgcagcaa aagcggagac cttggcgcca gttcacccgc gccggtttgg
    18121 ccctggccgc gcccctcctg ctctcgctct gggttcgtgc ggaagggccg ctcttgcacg
    18181 aatacgtgcc gaaggatgcg gtcgaagacc tcgagctgaa cgccaccacg cccgacgggc
    18241 gtatgccggt tgcgattcaa acccccaatg ggcccgccta cgcgccgaac ttgctcgatc
    18301 cgcacgcgcc ccgccaagtc gtgtacgggg acaactcgac gcccgacagc atcgacgcga
    18361 cgttccggct cgattcggat acgagtgagc cgtcgttcgt gcactacgac gatccgttca
    18421 caccttcggt ggcgcccttc aaacggctgt tcgcgttcga cgcggtcgac gccgatttcc
    18481 aattgggcgt gcgcgactcg aaggcgcttc gaccagtgcc gctgatgccg gaagcgccgc
    18541 cggaccatga ccgtttcttc ggcgacttgt tcgtcgacgt ggcccccacc gtgctgacgc
    18601 gcatccccac ggtggcaccg ggtacgcggc tactcgcgct gcgcgtggag cctgaaaccg
    18661 ccatcacctt gcgccaagat gcggccgaga actggttcgt gacgagcccg gagcgcaagc
    18721 gactacgctt gatcttcgag ctcagcgcgc cccgcgccgc gttcggcggg cctttcccgg
    18781 atgtctcgta cgaggcgatt tcacattcgg tgacgccgct ccccaacaac gtgcgccgcg
    18841 ccgtcgacga cgtgctggcg cgtttgggcc tttcacgcaa cgtgcgtccg cgcgacgcca
    18901 ttcagacctt ggtctcctac ttcaggtcgt ttgccccctc ggatgctccg cccaacgcac
    18961 gcgggggcgc ggcgctgtac accgagcttt cgctcgagaa aaaaggcgtc tgccgacacc
    19021 gcgccttcag cttcatgttg accgcgctcg ggctcggcat tcccgctcgg ttcgtgcgca
    19081 acgaggcgca cgcttgggta gaggtcggtg atggcaagac gttccggcgg atcgacttgg
    19141 gcggggccgc ccagcgcttc gatatcgata ctccgcccgg tgctccgcag catcgcatgc
    19201 cggaagaccc ctatgcctgg ccggaagggg cgcgcagcgc gctgcaatct accgagagct
    19261 cacccccgcc gaacgccaat ccgcgtccgg ccgcttcgac accgcgtccc gcgagctcgt
    19321 cgtccgcaac gagtcccgtg acgagcagcg cgcgcccggc tccaagcttt agtgctccga
    19381 gtcccgccgc taccccaccg gagggccagg attctccgtc acaaatcgaa ttcgcgatcg
    19441 agccgggaga cgtgcagcga ggggccaccg ttcgcgtgcg ggggcgggtc acgcaaaacg
    19501 aaagcccctg ccccagcacc cgcgtggaca tcgtcctgtc ggatggcgcc aaagacaacc
    19561 tcatcggctc cgtccccacc gacagcggcg gccagttcga cagccgagtc acgatcccct
    19621 tcgacgtgga gctcggtgat cacgtggtgc gcgccaccac gcctggcgcg ctacgctgca
    19681 atcctagccg ttgagggggg cgtctatcgt tgggcacggc cgagttccgt gcgcgttttc
    19741 acctaggctc gtgaagggca gcgccgtgaa gggcagagcc gtgaagggca gagccgcgcc
    19801 ggggctcgct cgcaacctcg gatgacggaa cggcgagccg tcttgcaacg acccgctccc
    19861 ggccgggtcg gctggagacc tcgcgccggg ccactatagg ttcgccctcg aagctctggt
    19921 aggctcccgc gtcatgtcca agacgctgga agcgcagtac caagctctgc cggaacaact
    19981 aaaagccacg ctcacccgct atggcttcga tttggcgcag tttctggagc tcgccagccg
    20041 cgtcaaggcg ggcaagatcg aagataacta cgtcaaaggc gaactccaac cgcccaagcc
    20101 cgaagacgtc cagagcctgc ccgcgaagga cacccccgct tacgcagagc tcgtcgcgct
    20161 cggtgaagag gagctcagag cgggccgggt cgcgctcgcc gtactcgccg ggggaatggc
    20221 tacgcgcatg ggcggcgtgg tgaaggcact cgtcgaagca ctaccgggca agacgttcct
    20281 ggacctgcgg ctgcaggaga tcgaaagcaa tcgccagaag tatggcaaag ccccgccgct
    20341 ctggttgatg gcgagctact ccaccgaggg cccattgcgg caggcgctcg gcgcgcgcgt
    20401 ggaccatgac aagatcggcc tgttcacgca gttcatctcg ctccgcgtca ctccggaggg
    20461 agagctcttc ttggacgcgc agggtaaacc gagcgagcac gccaaaggtc atggtgattt
    20521 cccggaggcg ctacaacgaa gcggcctgct gcagcgcttc gtcgacggcg gcggtcgcgt
    20581 cgtgatgctc accaatttgg acaacctggg cggcaccctc gatcccgcga tcatcggctg
    20641 gcacgtgaag cacgggcagc ccgtcaccat ggaggtcgtc gacaagctcc cgagtgatcg
    20701 cggcggtatc ccgatctacg tggacaacgt cctgagcatg ctcgaggagt tccgcatccc
    20761 gccgtccttc gacccggcca cggtgcgcgt gttcaacacc aacgtgttcc acttcgatgc
    20821 ccgagctctg aacgaactca agatgaactg gacctacttc caggtgaaca agaaggtaga
    20881 cagcaaaccg gtcgtgcaat tcgagcgcct ggtcaacgaa atcgccggca agctgcctac
    20941 gcgctatctg ctcgtgccgc gcgaaggcgt ggagtcgcgc ttcttgccgg tcaaggacaa
    21001 cgaagagctc gtgcaacgac gtgccgaaaa cgaagcggtg gcacgcgcgc gggggatgat
    21061 tgcgtgagga cactgcgttt cggtctgatc ggcctgagtt tgggcctggg ttcgctgctg
    21121 ccgagtgtgg cgctagccca gagcagcgtc gcgcgccccc tgcccttgcc atcgtacggg
    21181 cgcagcgtcg ccggaacgga tgacggcacc gccgtcgtca tcaaccccgg aaatctcggt
    21241 ttcatgccgg gccccgaggt gcgctggatg gggcgctttc tcgacgaaaa cgcgctgttg
    21301 ccgtatcaag gccacgcctt ctcgctcgcg acctcgctgc cgttcgggct cggtaccgcg
    21361 ctgcgcttgg acttggtaga tcctccggcg gcactcgcca gtttgggctc gcccttcgcc
    21421 gcgaactaca actggctcca cttcggactc ggctatcggg cttcggattc gttcggtctc
    21481 ggtttttcgt acaagcattc tttttcggac aaccgcctga cgaacgggat cgactcgtgg
    21541 tctttcggtg ccagcatccg ccccgcacga cgcttcggga tttcgctggt cgcgcacgac
    21601 gtgggcaatc cccacgcgcg ctccggtgat gcgctggcgg cgtcttacga cctggccttg
    21661 gccgtacgcc ccttcgccac gcgtgtgctc gagctcggcc tcgaatctcg ctacgtggcc
    21721 gaggagcatg gttacttcgt gccgcgcgcg acgctcgggg tcgatattcc cgagctcggc
    21781 cggctgcgcg gggagttctc ggtcgcagat ccgacggaag aggtgcaaac ccgacgttgg
    21841 ctcgccagcg cggtcatgag ctttggctac aacgcggtcg gcggggccat ggagttcggc
    21901 gccggcaccg tgttcggcga tgcgctcggt cgctccgccc gcggccaagc cgaagagaac
    21961 ctcgcgttcg acgtcgcctt caaaggttac cgtgaaccgc gtgcttccga gcctccgatg
    22021 cacgcgattc gcctgcggct cgagaacact cccgacaacc gcggccacgt cgcgctgctg
    22081 cgccggctct ggaacatcgc cgaacaggag cgctctgcgg ccgcagtctt gctcgagctg
    22141 cgcgcttccc ccggcaactc gctgccccac atccaagagc tgcgcgacgc gatccacaag
    22201 ctgcgcaccg cggggaaaca ggtcttttgc cacctcgaag acgccgacgg cggcgcgctc
    22261 tacctgtgct cggcggccaa ccgaatttgg atgtctccgg gcggaggcta tcgcttcgcg
    22321 ggcctcaagg cacgctatct gtacttcaag aacttgctcg acaacttggg tgtgcgcgcg
    22381 gatttcgtgc gcatcggtgc ccacaagagc gcgcccgagt ccttcatgca agaaggctcc
    22441 agcgacgtcg cgcgtcagga caagatcgac cttctgcagc aatacgagcg acagttcacg
    22501 ttgggggtcg cgagcgggcg caacatcgag ccgctcgaac tgcgtcgccg gatcgctcag
    22561 gggccgttca tcgcgagcga ggccaaagcg cagggcttgg tagatgagct cgccttcgac
    22621 gacgaggtgg acgcgctgat caccaagacc ctcggcgggc gcgtgccggt catcgacgac
    22681 aacagacatg ccgcctacgc tccctcgtca ttcggttcca cgcgcggcct gtgcatcgtg
    22741 tacgtcgacg gcgacatggt agacggccgt agccaagtca tcccgctgct cgggatgcgg
    22801 ctcgtgggct cctacaccat cgccgattcg atcaagaaag ctcgtgaaaa tccgatggtg
    22861 ggtgcggtcg tgctgcgcgt cgaatcgggc ggtggctcgg cgctcgcagc cgacgtgatc
    22921 tggagagagg tgcagctcac gcgccaggtc aagccggtca tcgtgagcat gggcagcagc
    22981 gccgccagcg ccgcttacta catcgccgcg ccggccacgc gcgtgatcgc caacccgctc
    23041 agcctcaccg gcagcatcgg ggtgttctac ggcaaggcgg atgtctccga gctgctgcgc
    23101 aagattggcg tcaatgtgga gctctacaag accgcgcccc gcgccgacgc cgaatcgttg
    23161 ttccgtccgt tcaccgccga ggagaagaag gaactcgagc gcaaggtggc gcagttttac
    23221 gagcagttct tgagccgcgt cagcgcgggc agacgcatga cccgcgatca ggtcgacgcc
    23281 gtcggccaag ggcgcgtatg gacgggcgag caagcgctgt cgcgcggcct tgtcgatcaa
    23341 ctcggcggct tgcgccaagc catcgagagc gcacgtgaac tcgccgggct cggtgaagaa
    23401 gccccgatcg tcgagttgcc gccggtggag acgagcctgc tcggcaagct cctcggcatc
    23461 gaaggcctgc acgccgaatc ggtggtcttg ccgccggagc tcctccgctt gggtcaggct
    23521 ttggcgccgt tcatggtcca tcccgccgag aaacctctct accgcatgga gctcaccgat
    23581 atcgcccctt gagatgcaag cacagcgggc cgaggcggta ctgctcgagc tacgagaagc
    23641 gcgctggcag ttcgcgagct ccgcgcagtc gaatgagctg tcggtgcgag ccgttggggc
    23701 gcgcgtggcc ctgatcggcg cgtgggacgt gctgtttcgg ggcctcgctg gtgaagcccg
    23761 cttgagcggt ggaagcgcct cgatctacgg ccagccgcca gaagtcgctc tgcggcaggg
    23821 cctggtgggc gtgcagcgct tcacgttacg tgcgccggca gacatcaccc ccgagcaata
    23881 cttgttcgag agcgcgagct tgctcgggcg cggcagcagc tacgcgcggc gcgaagccgc
    23941 ccgtgccgtg gacgagtacg gactgagctc ggtcaagcgc tacccgttca gcgccctacc
    24001 cgtgctcgag gcgcggctcc tcgccctggc ccaagctacg ctcggagagc cacgcgcatt
    24061 gttctgcgag ctaccgctgc atcgcttcgg cgaggctgct cagcgcatca tgctgagcgc
    24121 gctggagcgg gcggcgaccg ggcgaagtct actcttctcg gtagcgcaga gggtctactc
    24181 cgggcctcaa gcagagctct tgagccgcgc cgaccaacgc ttcgcgctcg aacgcgggca
    24241 gctgagcgag ccgtcggcat ctcccgcacc ccccaacggt gaagcttggc tgatcaccgt
    24301 ctcggagcac ccagaccgct tcaccactcg gctcgtcacc gacaagattg ggttcgaaga
    24361 gctcggcccc gtcgaggcca tggagaccct gttgcagggc ggccgtagca cgccggtccg
    24421 acgcttccga gtctggttgg aaggcgcccc cgctcgggag cgccttctcc acgccgcgcg
    24481 cgacaccgaa gcggcgatcc tcgaacttcg ccccgagact tgacgcgcgt gggctcgaac
    24541 gccggagcgc cgcttcagct ctgactgagc cgcttgcgcg gagcatccag taacgtggcg
    24601 tgcgcttctt tcagcgcgga gatcacgaag tccggcttca ggtagctgtt tgcgagcacc
    24661 cgccgcaacg ccggttcatc caacttgccc gcggtttctc cgggaaacca gtcgtcaccg
    24721 cgccccagca agttgtaacg catcttcccc caatccacga gatcgagcga cttcgaatac
    24781 acccagagcc gcacgatctc tcgcacgttc acctcaccgg gcacgtcgac gtactcggga
    24841 atgccctccg cataacgctc gcacacgtca cgcccgagca cgcgccacat ctcctgctcc
    24901 acgcgcgcgc gaatcggcgc gatcgtctgc tcgatccgat cgtaatgctc gagcgcggcc
    24961 acgtgctcgt cgaaatcact cgggcgagcc gcgccgatac tcaacgtgtg cacttcgggg
    25021 tgcgacaaac agtacaagtc attgaactgc atcggcgtga gcggccgaca caactccacg
    25081 agcttcgccg gaggcgtata aagcttcccg cccttgtcgt tcgggctgat gatgaacacg
    25141 cccatgtcgc gtcgatgcgc cgcctgcacc gccgcccaat tcagcagatt cacccaataa
    25201 aagtgcagat tcacgtactc gaagccacca ctctcgatgg cttccaaaat caaatccgtc
    25261 gtggcgtgcg tggaaaatcc gacgtgcttg gcgagccctt gttccttcag ccgcagcgcc
    25321 gcctccaagc acccgccctt acgcaatgcc caatcgagct tttggcgatt gttgatcccg
    25381 tgcagcgaga gcaactccac gcgttccagc cgcagattgc gtagcgactg ctcgaagttc
    25441 gccacgaact cgctcgtctg cgcggtgagc cccaccttgg tttgcaccag cagctcactt
    25501 cgcggaaaac tcggcagaac ctgcccgagc tgcagctccg aagtaccgta accgcgcgcg
    25561 gtctcgatgt gattcatgcc gagctcgagc gcgcgccgga tggtggcttc caagttggtc
    25621 tgcgaatccc tcgggatctc tcccaaaccg agatcgctcc acgagtgctg gaagcgcatt
    25681 cccccgcacg tcagcacggg catctcgagc tctgtctttc cgaaacgtcg ataacgcatg
    25741 gccaccttcg gtggcgcgag cttgccacga ctcgcacact gcagccaaac cggcttgacc
    25801 gcaggtgatg tcggccgcgc tgtaaagagc gcgccgcgcg ccgccgctgc ccccgagcgc
    25861 agcctcttac ctagactccg cccccgtcac cgcgccggcc ctgcgaataa agcggcaatt
    25921 cctcgaatct cgtgagcgat tttgcataac gcggtttcac gcgcattcac gcgcgccggg
    25981 cggcaatcgc ccgcacactc tccagcgctt cgtcgacgtg gcgtcgtgcg gccagctgcg
    26041 aacggaatac gtgacgcacc actcgctcgc gatcgatcac gaacgtgacg cgccccttga
    26101 gcaacccaag agttttgccg accccgtatg cttcgcgcac tcgcccaccc acgtccgcga
    26161 gcaacttgaa ctcgaggcca tgacgttggg cgaacttgtc gtggctctgc acgctgtcgt
    26221 cactcacgcc cacgacctgc gcgccagcct cttggaaagc gctgcggtcg tcgcggaagt
    26281 agcaggcttc ctgcgtgcaa accggagtgt catccttcgg gtagaagtag agcacgagcg
    26341 gcccctgctc acacaaaccg cgcagggttt ggcgctggcc gctctgatcc gaaagctcga
    26401 aatccggggc cgtatcgccc acctcgatgg tcttcgccaa tgtttctctc cgctgggctg
    26461 ttctgctggg ctcgcctgtt tgctgggctc ggctgtttgc tgggctcggc tgtttgctgg
    26521 gctcgcctgt tttgctgggc tcgcctgttt gctgggctcg cctgcctgtc cggttcaagc
    26581 tgtatcaggc gcgcggcgac gcgaccagcg cgcaactggg ctcacgtgct ggcgggactc
    26641 ggttactgag gcagaggcag gcagcctgcg gaggccaaga ggaatatcgc gcaatcctgg
    26701 gcaactcgtt cgaacgcctg ctcgcacgtg agcccctctt gtttgcgcat cacaccgtcg
    26761 acacacgcga acaggaactc gctcgtttgc agacactcat ccgtcgcttg cagcagaggc
    26821 accgagcacc cggcattgca tgtttccatc ggcgacttta cggccgtacc gcacgcgtcg
    26881 atgatactcg cgcatgcgct cgagcaagcc ttcaccgcgc gatccaacgt gcccggacgg
    26941 cccccggtcc ccgtgttccc gcccttccca cctggttcgc cgccgctgcc cccgaaagct
    27001 tcgccgccca ctccaccgct cggggccccc gcttcgggtg cctgccctcc gtcggcagct
    27061 cgcccacccg tattctcgcg cccgccggtc gcacgttgcc cgccttgagg tcgcactccg
    27121 ccctgagcca aaccgcctga gccaaccgca ccgcccgcgc ccactccgcc ggtctcggca
    27181 aagaacccag catccagcgt acgaccaccg cacgccgcca agacgcagca gggaaccaga
    27241 actaggatcg agaccgtaac gcgtgccgcc cgcataccgc gtaggttacc cggtttgcgc
    27301 cacgttgaca cgccttcact gccttcactg ggcgggagcg cacttcgggc aacccgaaag
    27361 ccggtctatt tcgcgcgcgc gaaccagacg ccgctctgat caccattttg tacggcgtag
    27421 acgacgccag cgtgcggcca gggcacgagc atgaactcgg agacgttgtt cgagacgatg
    27481 ccccgagcgt cgatctcgac gttccagtat tcgaggtttc cggttccttt ttcgaagttg
    27541 tattgcgaaa agaaggccac cccgggcagg ccgaatccag tcaatgaaac gaacccccac
    27601 ttcgggaccc ccgagcccgc tgattgaccg atgcgcggac cagaggccat cgcgagggtg
    27661 ccgcgcgcgc cgtcgaagtc ctgcagaacg gcgctgcccg agcctgtatc cgcgatgaac
    27721 ccgccgtcca gtggcacctt cgccgccgcg aagtttacgg cagggacggg gctctccgag
    27781 ccgctcggaa tccgatcgac ttgcacgagc tcgccagttc ccgtcgccac gtccgcgttc
    27841 gacaacacga agccatcgac gccgtagttg gacgcattcg ggatttggcg cggcaccccg
    27901 gtcaccaact tgtgctccgc gaactcgcga tcgatgaaca tcaaagttcc gatgccgtcc
    27961 cgcacctttg ccaggacgta gacgccctcg ctctgcacgc tgagccagcc tagatccgct
    28021 tgctcggcca aaagccgcgg ctcaggcgca tcgatcccat ccgcttccgc ggtgcttcct
    28081 tctggtaggg gctccgtcac gtacagtgat ccgaggcctg tattcgggtc gatgtcctgc
    28141 aagtagtaga gctggtcgat gaactggcca ggccgcttat cgtccggcaa ggagacgaac
    28201 ttggtcaaac tcgggtgcac gctcactccg aactctagag gaccacgcga cgcgctgcgg
    28261 gtcttgagtc ggcgctcttc gcaaggagag aaatacgtaa tccacttacc cccggtggcc
    28321 tcgcaagcgt cggtcgcaaa aggcgtcagg ctcagatgcg ggcccgcgct ggcgctcaag
    28381 atgctcagac cgtctgcgtc ctcgagcagc agagaggaat ccgacaaccc aaccaagcgg
    28441 cgaatctctc gcccgtaggt gccaagctgg gtccaatccg cgtcgaacgc ggtgagcgag
    28501 cggtcgattt gtaagagcca caagtaattg ggaaccttgt cggacgctcg gaaagacagc
    28561 agccgaatgc cctcgaactt ctcgacgatc gacgtctgct cggacgtcag cgggtccgcc
    28621 cgcacgatgc cgtcccccac ccgaatcacg gcttcaccct tttcggtgat gtcgaacggc
    28681 aaacgcgctg acgacaattc gaagttgagc ggttcgcact gtggagtcgc gaagcgcaga
    28741 cgaccgaagc cgttctcgtc cgcctcgctc tgataggcaa tccaaggtgg cacccaatgc
    28801 cggtagttca cggcaccggg gagctcgcac atcttttgac gttgtggatc gaccaccatc
    28861 aggtcggaag cttccgcgtc cgatcgacgc gcgaacatca aatccgcctg atagtccaga
    28921 ccgtaaaact tgccgggcaa caactgatag cccggcccgt cgatatacag cgtgtcttcg
    28981 ggggacagcg cgtcggaggt atctcggaag cccttctcga gactgcagcc cgcgagcgcc
    29041 agaacgcaca atagaacccc tcgtatcgac gcccgcccac gtgtcagcgg ctctgacctg
    29101 agcgacggca gtggctcgag ccgacgtcta gacctcaacg tagagcagag ggaaaaaacg
    29161 agcgagcgac agcgaagaga agagacaccc gaagacccgc gtgccatgct gggcacgagc
    29221 ttagcatcgg atgggccaac gtgacgtggt tctacgtctt cggatgcgaa gtagtttaga
    29281 gaaggatccg tgagaggtcg gcgttcaaat ccagcgttga gaactttagc ggcatggctc
    29341 ttcggtgtga gcgcggaagt tgcgatgtgt tcagcggcgt gggcacaacc tggcacaccc
    29401 gcggcggatt ccgcgactcg agcgccagag tctgaatttt ccgctccaga acccctacaa
    29461 gccagcgtcg aatatccggc ggatgcgagc ggtcgggcgg aggttctgtt ggagttgacc
    29521 atcgatcccg aaggtcatcc ccgagacgtg gcaatcgtgg agggcgctcc ccccttcgcc
    29581 gaagccgcgg caactgccgc agctcaatgg cagttcaagc ccgcgcgtct tcgcgggcgc
    29641 aacgtggcgg cacgcattcg ttacacggta cgcttcgagc ccgagcc
//
LOCUS       DASTKU010000727         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_363563, whole genome shotgun sequence.
ACCESSION   DASTKU010000727 DASTKU010000000
VERSION     DASTKU010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363563"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFQ61_25425"
     CDS             <1..466
                     /locus_tag="VFQ61_25425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957874.1"
                     /translation="TGGKMCTEGTTAKVLKTSAGADDYSNMWGAGIGMDFNHPPDPAT
                     KMPFDAVAAGIKGVSFDLDMPVVSSLRVEFPIPETEMHADGSAYWGATKSWPASPVSV
                     GTNTITWDKVQQPNGTIPFNPAKLLGIQFHVPTNTSAATPYSFCISNLKLIK"
     gene            complement(584..1303)
                     /locus_tag="VFQ61_25430"
     CDS             complement(584..1303)
                     /locus_tag="VFQ61_25430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957875.1"
                     /translation="MFRSIVMRPGVGSATVVIIALRQDGELFVSTRDGRTGTFSSWLS
                     IGSPFDIATGERLALRQISNTWQVVGPGPQIVADVFAVSESGVLYRLVGWGGARWERT
                     QQLADIAISSSAAGTAIFGYHSSGLRWGNPAVPPIAPRMTFQWNFQTTYGPALPAAVG
                     SRFLWLLDADATNVDGRILRSTKQSDGSWGPLQVFATGEDGTSSGQNRHWFIADGSAF
                     RGNSGELFVVGEKFHLRNYVP"
     gene            complement(2065..2733)
                     /gene="pgsA"
                     /locus_tag="VFQ61_25435"
     CDS             complement(2065..2733)
                     /gene="pgsA"
                     /locus_tag="VFQ61_25435"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET9957876.1"
                     /translation="MSDPTRSSPENSLAGGRRRMAPGTLKRDAMNLPNLLTFGRVLII
                     PLVLALLDRGTPKDCAWAAIVYSAAAITDLLDGYLARRMNVVSVLGKFLDPLADKLLV
                     MASLVYMVPMGRISEWAVALLLAREIAITALRSIASSEGVVIAAGDDGKSKTALQMVG
                     ILCLILGYPYHLTLGPIDMGVVDLVYVGRALVYVSLVFSLLSAVSYVKTFAAAVEAKA
                     EREG"
     gene            complement(2764..3486)
                     /gene="hisA"
                     /locus_tag="VFQ61_25440"
     CDS             complement(2764..3486)
                     /gene="hisA"
                     /locus_tag="VFQ61_25440"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HET9957877.1"
                     /translation="MELIPAIDLLEGRAVRLRQGRYEEATIYSDKPEELAREWSGRVT
                     RLHVVDLEGAKAGQPVQREQVRRIVEAFGPGVQLGGGIRTLEAVSTYLDLGVTRLVLG
                     TAAVRDPDFVARVAEAHPNCVILAVDARAGRVATSGWLEATELLAVDLVKQLKHLPLA
                     GVLYTDIERDGTEVGPNVAETARLARETGAAVIASGGVGTLDHLRALAGAGENIIGAI
                     VGRALHERRFTLEQGIAASSGA"
     gene            complement(3517..>4027)
                     /gene="hisB"
                     /locus_tag="VFQ61_25445"
     CDS             complement(3517..>4027)
                     /gene="hisB"
                     /locus_tag="VFQ61_25445"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HET9957878.1"
                     /translation="SDIVTPLPFLTHMVDQIARHGCIDLKIRAEGDVEIDGHHTTEDL
                     GIVLGAAVLKALGDRAGIRRYGSATLPMDEACVTCAMDLSGRPFFVFEVPIAKAKLGT
                     FDTELCEVFFEAFARSCQANLHFHLLAGQNLHHIIEISFKAFARALRQATEFDPRAPG
                     IPSTKGSLD"
BASE COUNT          807 a         1263 c         1214 g          743 t
ORIGIN      
        1 caccggcggc aagatgtgca ccgagggcac cacggcgaag gtgttgaaga ccagcgccgg
       61 cgcggatgac tactccaaca tgtggggtgc tggtatcggc atggacttca accatcctcc
      121 cgatcccgcc acgaagatgc cgttcgacgc agtcgcggct ggcatcaaag gtgtgagctt
      181 cgacctcgac atgcccgtcg tttccagcct gcgcgtcgag ttcccgatcc cggagacgga
      241 gatgcacgcc gacggttccg cgtactgggg cgcgaccaag tcttggccgg catctccagt
      301 gtccgtcgga acgaacacca ttacttggga caaggttcag cagccgaacg gaactatccc
      361 gttcaacccc gccaagctgc tcggcatcca attccacgtg ccgaccaaca cgagcgccgc
      421 gacgccctac agcttctgca tcagcaacct caagctgatt aagtaaccaa agtaagcgaa
      481 agcgtgcctc tgggaaaacc ctcgagtcca tgacccgagg gttttcttgc atgtgcattc
      541 ggaggcggct ccacttcggc ggcagcgttc cgtgtccgcg ccactacggt acgtagtttc
      601 gcaagtggaa cttctcgcca accacgaaca attcgcccga gttcccgcgg aaggcggagc
      661 cgtcagcgat gaaccagtgt cggttctgtc ccgaactcgt cccatcctct ccggttgcaa
      721 agacttgaag ggggccccag ctcccgtctg actgtttcgt cgagcgtaag atccgcccgt
      781 cgacgttggt cgcgtctgcg tcgagtagcc acagaaatcg cgagccaacg gctgctggaa
      841 gagcggggcc ataagtggtt tggaaattcc actggaaggt cattcgcggg gcgattggtg
      901 gaacggcggg gttgccccat cgaaggccgc tcgagtgata gccaaaaatg gccgtaccgg
      961 cggcgctcga cgagatagcg atgtcagcga gctgttgcgt acgctcccac ctcgcgcctc
     1021 cccagcctac caaacgatag aggacgccgc tttccgacac agcgaacacg tcggcaacga
     1081 tctgcggtcc cggaccaact acctgccacg tattcgagat ctgtctgagc gcgagccttt
     1141 cccccgtagc gatatcgaag ggactaccga tgctcaacca cgagctaaac gtcccggttc
     1201 tgccatccct cgtggaaaca aagagctcac catcttgcct gagggcaatg atgactaccg
     1261 tcgcgctgcc cacgcccggt cgcatcacga tcgaacgaaa catcttcccc gacgacacgc
     1321 tgcgagcaaa tgcacttccg tctgctgggg cggatgttcc ggtcagctcg ctcattcccc
     1381 gtggttgcca gcaggtcaac ccttgaccga acgggccctg gccgtcattc ggtgggctgg
     1441 tccagcacac gaaccctctt tctactgcga ccatgctcgc ttgtggatgg ccggaagcga
     1501 gatcgcgcca gcagaagcgg tcgttcgcgt ctccttggtt gcatagtcga gcctctgcag
     1561 aacccgcaaa tagtgccgcg tctgcggcca aagtcccgac aaacagaagg cgacaagccc
     1621 tcgttgcaaa tcttgtaaac attccattcc cctagtgttg cgctacgaca agattatcct
     1681 gacgtcgtct agcaatcgct gaataacagt cagctcaatg actggcaagc tcgtctacct
     1741 tatgccgtta gcccagagcg atatgcaggt agaaaccagt attgcgcttg tgctttgcct
     1801 tggcttgcct caacgattgc caatcaggtc tttggcttac caagtcacgc cagagctagt
     1861 cacgaacaga gtctgtgcaa cgcttagagc actgactccg ttgcgtccga gtgggcttca
     1921 cgcaagcatc gaggcgaaca tgtgcagtgg aataggcacg cgcaagtcga acgcgcggtg
     1981 acgaacaaag ttcggcgcta gtccgcggag gtgctgaacc aagcccaacc taaactcagg
     2041 atatacgcgc gaaactgaac ggggtcagcc ttcgcgctca gccttggctt ccaccgcggc
     2101 cgcgaaggtc tttacgtagg agacggcgct gagcagggag aagacgaggg agacgtaaac
     2161 gagggctcgg ccgacgtaca ccagatctac gactcccatg tcgatggggc cgagtgtgag
     2221 gtgataggga tatccgagga tcaggcacag gatgccgacc atctggaggg ctgttttgga
     2281 tttgccgtcg tcgcccgcgg cgatcacgac gccttcgctg ctagcgatgc tgcgcagggc
     2341 ggtgatcgcg atttcacggg ccagcagcag cgcgacggcc cactcggaga tgcgccccat
     2401 cggcaccatg taaacgagcg acgccatgac gagcagtttg tcggcgagcg gatcgaggaa
     2461 cttgccgagc acgctcacga cgttcatgcg ccgcgcgagg tagccgtcca acaaatcagt
     2521 gattgctgct gccgagtaca cgatggccgc ccacgcgcaa tccttcggcg tgccgcggtc
     2581 gagcagcgcg agcacgaggg ggatgatcaa tactctgccg aaggtgagta ggttcggcag
     2641 gttcatggcg tcacgcttga gcgtgccggg cgccatgcgg cggcgaccac cagcgaggct
     2701 gttttcagga ctcgagcgtg tgggatcact catggtttgg agtcgcctgg gagggtctgg
     2761 cgctcaggca ccgctagacg ccgcaatgcc ttgttcgagc gtgaagcgac gctcgtgcag
     2821 ggcccggccg acgatggcac cgatgatgtt ttcgcccgct cccgccaacg cccgcaaatg
     2881 atcgagcgta cccacgccgc cactcgcgat tacggcggcg cccgtctccc gagcgaggcg
     2941 cgcggtctcg gcgacattgg ggccgacctc ggtcccgtct cgctcgatat ccgtatatag
     3001 aactccggcc aagggcaggt gcttgagctg cttgacgaga tcgaccgcca gcagctcggt
     3061 ggcttcgagc cagccgctcg tcgcaacacg cccggcacgc gcgtctaccg cgaggatgac
     3121 gcaattcggg tgagcttcgg ccacacgcgc cacgaagtcc ggatctcgaa cagccgccgt
     3181 accgagcacg agccgggtca cgcccaggtc caagtaggtg ctcaccgctt cgagcgtgcg
     3241 gatcccgccg cccagctgca ctccagggcc gaaggcctcc acgatgcgcc gaacttgctc
     3301 gcgctgcacg ggttgccccg ctttggcgcc ttctagatcc acaacgtgca gccgcgtgac
     3361 gcgccccgac cactcgcgcg cgagctcttc gggcttgtcg gagtagatcg tggcctcttc
     3421 ataacggccc tggcgcagcc gtacggcgcg gccttccaac aaatcgatgg cgggaatgag
     3481 ctccatttct gttccgtcct attcgttccg actcggtcag tccaagctgc ccttggtgga
     3541 agggatccct ggtgctcgag gatcgaactc ggtggcttgt cgcagggcac gggcaaaggc
     3601 tttgaaagag atttcgatga tgtggtgcag gttctgcccc gcgagaagat gaaaatgcag
     3661 gttcgcctgg caagaacggg cgaaagcctc gaagaagacc tcacacaatt cggtgtcgaa
     3721 ggtacccaac ttggctttgg cgatgggcac ctcgaatacg aagaacggcc gcccggaaag
     3781 gtccatcgcg cacgtgacgc atgcctcatc catcgggagg gtggccgagc cgtaacgccg
     3841 gatccccgcg cgatcgccga gcgccttgag caccgcggcc ccgagcacga tgccaagatc
     3901 ttcggtggtg tggtgcccgt cgatctctac gtcgccttcg gctcggatct tcagatcgat
     3961 gcagccgtgc cgcgcgatct ggtcgaccat atgggtcagg aacgggagtg gcgtcacgat
     4021 gtcgctc
//
LOCUS       DASTKU010000728        12493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1033203, whole genome shotgun sequence.
ACCESSION   DASTKU010000728 DASTKU010000000
VERSION     DASTKU010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033203"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VFQ61_25450"
     CDS             complement(<1..993)
                     /locus_tag="VFQ61_25450"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9957879.1"
                     /translation="MRSLKTLVALGIALGGTLACGGEDPCKPDDKKLDTTGEPNTSGG
                     TGSGGTGPSATASAGTASGGIAAEGSRTGGSGSGGRTGSGTSIEGGAQGSTDEEGGSQ
                     ATGGVASGGLSGSGSGGATTPPVKPLPGDVVFEEGRVLEFRMSLSDADWLHLEEHGDD
                     EEYLPASATIRGDGFAETKFTRVGLRHKGAYSLERCFDNPDGVREDSAECRKLSYKIK
                     FDEYVSESRLDGLKRINLHASATDPTKLRELLAYDTYRSFGVDAPRARPAKLYINDQF
                     QGLFIAVEEIDGRYTKAHYPEGPDGNVYKEIWPHPDQTKESYQAALETNEDEADV"
     gene            complement(1805..3184)
                     /locus_tag="VFQ61_25455"
     CDS             complement(1805..3184)
                     /locus_tag="VFQ61_25455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957880.1"
                     /translation="MKRLRWGALSLALCVSGVALGCGKTHSSRAPSGEPSDQMTGGAT
                     DGGADGTAPGAGTGAKNSSGGRAGTGATGTGASGSGARAGVGGNSVVGGNSVGGEMSV
                     VAGANSESGGRSGGATGGSSSVEAGSPGTSELEGCTAPTSVAVQCAVPEPERGATPVQ
                     QCIQKPKLVTDERYIKDVFADSMGVYWANDADELRALETGQSAPTTLFASQQGTIGNI
                     VGNDQSLFFLVWDSASKANQSSSIHELSRVNGAHRKLDSDLPLNVSLAVQGQNLYLGN
                     VPGPGDLSVHSIAGVGPAPRSIAESAVMQLTADETHLYWITEAGALQRLELPDGAPET
                     LTSGQVRLRDLRVSNSEVFAISDSGVLSVSTAGECARALVLPPWVLDLSGLAVDDDFV
                     YFGSVMESDLLHSSIWRTPRAGGYAVQMTPMSSETGSGSAAPLVISGRRLFWRTQGGL
                     EALTLPGAP"
     gene            complement(3496..3885)
                     /locus_tag="VFQ61_25460"
     CDS             complement(3496..3885)
                     /locus_tag="VFQ61_25460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9957881.1"
                     /translation="MADTSVTKVDSAHSPRGAMGQFYLASGVQLGMRLWRDEPVTAQS
                     EVATQADETARTKTVRRDYEVVGYVLQGRAELEIEEQKVVLKTGDSYVVPRGAAHRYR
                     ILEPFSAVEATAPPAHVHGRDERGSAE"
     gene            4079..4753
                     /locus_tag="VFQ61_25465"
     CDS             4079..4753
                     /locus_tag="VFQ61_25465"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET9957882.1"
                     /translation="MVTRARDSATRRDDMVVRQLAARDITDPRVLQAFRDVPREAFVP
                     KSLAHEAYEDRPLPIGQHQTISQPYIVALTLQALNLTGSERVLEIGTGSGYSAALLSR
                     LAREVYSVERLPSMAESARGRLASLGYRVHVKVGDGTLGWPEQAPYEAIAVAAGGPRV
                     PEALVAQLASGGRLVIPVGGDEGQVLLRVLQIPGDGLQHEPIEHVRFVPLIGVQGQPE
                     RTASRA"
     gene            complement(4802..5887)
                     /locus_tag="VFQ61_25470"
     CDS             complement(4802..5887)
                     /locus_tag="VFQ61_25470"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET9957883.1"
                     /translation="MTEPIGKRSVTALVLSLLAASCAQQDDTALLLAALKEHAAPPET
                     STEGGRQVAINPRLLRRFEPLRAQIEHPQNPITDDKVELGKLLYFEKRLSKHGDLSCN
                     SCHPLGKYGADQRATSLGTDGQLGKRNAPSVYNAAGFFAQFWDGRAQTVEEQATGPIL
                     NPVEMGLPSAEAGVKLLKSIPEYVRRFQAAFPTEKDPVTFENVGRAIGAFERRLMTPS
                     RWDEYLRGSESALTELELEGLRTFTNVGCMVCHTGEFVGGGMYQKVGVVEAWPNQSDQ
                     GRFEVTHQDVDRMMFKVPSLRNVAQTAPYFHDGSVANLEDAVRLMGKHQLGLRLSDRE
                     VTAVVAWLNALTGPLPALASSEPASPE"
     gene            complement(5880..8186)
                     /locus_tag="VFQ61_25475"
     CDS             complement(5880..8186)
                     /locus_tag="VFQ61_25475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET9957884.1"
                     /translation="MMGMQSGQIEPPRTRPSTPAPPLRRVDEEPRLISVPIPAPPATV
                     DVLVPSRRIRTKLVVLIAAMISLLVVGLAAYFPSRQLKQQHNEVIERAGVYAGLSSHQ
                     LRSAVAFEDQETAREVLESIARDASVDGIAVYTSQGRELYRAGQLSALAHAARRGFGG
                     PRVFSLPGRVLATAPVRSLEGPRGTVVIELSLRGVTQARDRLVWLALTAGLVALAVGT
                     TLAWLIAGSLARRIERIAEAATRVAGGDLSQHVRDDGSQDELSLLGQSFNTMVSRLNS
                     LITQIRESAREESHRLDRLVRERTAALDRKNRDLRLVLDSVDQGFVTIDQNAHVVGEY
                     SNAVERWFGPMAPDTMLWSYMSGRDTAQRESFEMGWQQLVEGILPLELTIHQMPRSIE
                     VDSRTLELEYKPLLADAQEAARLLVVMTDVTARRAQESREQEERDTMAVTSRLLSDRQ
                     GFVEFFKENSALSQRIFANQTDEVALKRDLHTFKGNTALYGMTGIARICHDLESCLES
                     VSPAELDCAPLERAWTHIHTKVEQMLGSRVEGRVEVAEREYLGVLSAVQRRVDYSTVE
                     KMLAVWRLEPISERLNRVAEHLAASVVRAGKGTASVRVDAPQLYLDRDELAEFWGVFS
                     HVIRNAAVHGTEAEADRRELGKSKAAEFALSAGLERGSFFIELRDFGPGIDWERVRKR
                     AAEQGFPHTTAEDLERALFTDGLSTADATTEAAGRGVGLSAVRDVCQRKRCKISIASS
                     RGRGASFRFDFDAASLTSLTQLEPRHYD"
     gene            complement(8227..10167)
                     /locus_tag="VFQ61_25480"
     CDS             complement(8227..10167)
                     /locus_tag="VFQ61_25480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9957885.1"
                     /translation="MTVAKGTSAQVEQHVLDNLLEGCQIIDSEWRYVYINPALEAQIG
                     RGREELLGRTMMECYPGIEHTQMFAELQRCMASRAHHQFENEFVHPNGARGFYELRFV
                     PIPDGMCVFSCDITRRRLSEQSLRQSQETLAELHTALPDAVLLVAGDGKILMANRAAE
                     RFFGERRVTRVKLRDLIRSQARELDLSTLLHAKGVEVKGAEAKAAAEFKVTRSPVMHE
                     ELSWVGVREDGVEFPVSVAVEPTLTGGERAFVALVRDMTEVRSLERQLRQAQKLEALG
                     QVASGVAHDFNNLLSVILSYSSLLLEDSALPERLRSDVNAVHVAGLKAAELTRRLLTF
                     GRRQVLQPQVSSLNDILTHFEPVLSRILGDDVELELVLSSELGLTRLDSRQVEQVIVN
                     LVMNARDAMPNGGKLTISTRNMDTVQLEGVSGLLTNGPHAVLTISDTGVGMDEATRSR
                     VFEPFFSTKSRGSAAGLGLAAVFGVMKQSGGHVDVVSALGKGTTFHLFFPRCDEPHTS
                     SPSGPSLTPISRRQGTETVLVVDDEEQVLALATKVLRRTGYEVYEAHGGGEALELCAL
                     HAGKLQLLITDIVMPKMSGPELAERVLAVCPQIKVLYMSGYPDDLMVKSGVVDPNVAF
                     LQKPITPEAFGRKVREVLDRSA"
     gene            complement(10488..11090)
                     /locus_tag="VFQ61_25485"
     CDS             complement(10488..11090)
                     /locus_tag="VFQ61_25485"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET9957886.1"
                     /translation="MPDLTIYLCRHGDTAWSAERRLAGRTDLPLIEQGERNAVQLGER
                     LKAVPFTRVLVSPLQRARRTAELAGMENVELEGRLVEMNFGRYEGLTVNEIRRESPGW
                     TYLREGCPEGESAEDLGRRADALLHELAPLRGKVALFAHSVMLRVLAARFLGLVPEAG
                     RNFMLSPGSISILSHDPVEDSPVIATWNDRAHLTGQDFLP"
     gene            11829..>12493
                     /locus_tag="VFQ61_25490"
     CDS             11829..>12493
                     /locus_tag="VFQ61_25490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957887.1"
                     /translation="MVPPNDDQAPYLLVHAARRYFIGLPLVFNVVFENRSAIAHFLRL
                     PRLDLLTRQANQFAVTLERIDKSEPARYVRFPPVEEDGPVAQLGPMERLSVICDLSNL
                     AVTFTPGIYRITFSVGRGPTERSSAPIEVALEALAPTDARVVGRLLQPNPRGRADIGA
                     WASFLEHHSRPIGLPLSLSQDVRKQLALHLFLSAAVHGRAPISELDADALGAITDRAW
                     LSEA"
BASE COUNT         2331 a         4222 c         3555 g         2385 t
ORIGIN      
        1 gacatccgcc tcgtcttcgt tcgtctccag ggcggcctga tagctctctt tcgtctgatc
       61 cggatgcggc caaatctcct tgtatacgtt gccgtcgggt ccctcgggat agtgagcctt
      121 ggtgtagcgg ccgtctatct cttccacggc aatgaacaag ccctggaact gatcattgat
      181 atagagcttc gcagggcgcg cgcgcggcgc gtccaccccg aacgatcggt aagtgtcgta
      241 cgcgagcagc tcgcgcagct tggtcggatc cgtcgctgaa gcatgcaggt tgatccgctt
      301 cagcccatcg agccgagact cggagacgta ctcatcgaac ttgatcttgt aggaaagctt
      361 gcggcattcc gcgctgtctt cccttactcc atcggggtta tcgaagcagc gctccaaact
      421 ataggccccc ttgtggcgca gcccaacgcg cgtgaacttc gtctcggcga aaccgtcacc
      481 gcggatggtt gcgcttgcgg gcagatactc ttcatcatcg ccgtgttctt ccaaatgcag
      541 ccaatccgcg tccgacaagc tcattcgaaa ctcgaggacg cgcccctcct cgaaaaccac
      601 gtcgcccggc aggggtttca ccggcggcgt cgttgcaccg ccactgcccg atccggatag
      661 tcctccgctc gccactcctc ccgtagcttg gctgccgccc tcctcatcgg tagagccctg
      721 ggccccgcct tcgattgacg tgccgctccc ggtccgcccc ccgctgcccg aaccacccgt
      781 tcgacttccc tccgcggcaa ttcccccact cgccgtgccg gcactagccg tcgcactagg
      841 gcccgttcct ccgctgcccg ttcctccgct ggtattgggc tcacccgtcg tatcgagctt
      901 cttgtcgtcc ggcttgcatg ggtcttctcc accacatgcg agcgtgcctc ccaacgcgat
      961 acccagcgcg actaaagtct tcaagctacg catttcgccg caccatacca cgccattcaa
     1021 cggcggagat atggactttg aatcatcaca cttcaagccg cgatagaagc aacactgata
     1081 tatctcgaaa gcacgtcggc acgacgaatc gtttccggag ctcgcgcgga ctgcttcgct
     1141 cgtgtttacg agcgaacttc gattcgccac ttcgagcatg cgcgccgcac gaagccgggg
     1201 ccaaatcacc cttgggtaag atcgcccgcc acgtgccgtg cacaatcctc acccagtcat
     1261 cgccgttggg gcggccgatg aatcgagcac ctgcacggat ctcgaatgta ccgtgcggcc
     1321 gcgatcgaac gagcgcgacc ccgcgtcgat cattcgtcgg atgcgcagcc cctgccccgc
     1381 ccctatccct cgcgaaaccg cgccagcccc ctcgatccag tgccaacgag ctagccccct
     1441 cgctcgctcc cgctcatctc ccaccaagcg cagcgcatac gagatcgtga cggtgccgga
     1501 acacgcaata ggccggagct tctgtcaaca cgtctttggt tcgatcgacg acgagcaatg
     1561 catttccaaa gcacggccac ggacgctgct aggtcgaaga ccgttcaacc gaggaccaat
     1621 gcccggaaac cgagcacctt gacgcgctcg gaatcagaaa gtctcatgcc gcgtgaaagc
     1681 cgcggcaccg aaaggcccgc ggccacaccg ggagtattct tgtcgctagc aaacgactcg
     1741 cccccttgcg cgcgtcgtta gccccaaagc cgtgctgggg agccggagaa tcgagccaat
     1801 ccttttacgg cgctcccggc aaggtcagcg cctccagtcc accctgagtc cgccagaaca
     1861 gacggcgtcc cgatatcacc agcggagctg cagaaccgga gccggtttcg gagctcatcg
     1921 gagtcatctg aaccgcgtag cctcctgcac gcggcgtccg ccaaatcgag ctgtgcagaa
     1981 gatcggactc catcacggaa ccgaagtaga cgaaatcgtc atcgaccgcc aatccactca
     2041 aatcgaggac ccacggcggc agaaccaaag ccctcgcaca ctccccagcg gttgaaacgg
     2101 acagcactcc gctatccgag atggcgaata cctcggagtt ggatacacgc aaatcgcgca
     2161 ggcgaacctg tcctgatgtc agcgtctccg gtgcgccatc gggcagctcg aggcgttgca
     2221 gcgcacccgc ttcggtgatc cagtagaggt gcgtctcgtc cgcagtgagc tgcatgaccg
     2281 cactctccgc gatgctacgt ggcgccggcc ccaccccggc aatcgaatgt accgagagat
     2341 ccccgggacc gggcacgtta cctagataca gattttggcc ttgaaccgcg agacttacat
     2401 tgagcggcaa gtcagagtcc aacttgcggt gcgcgccatt cacacgagaa agctcgtgga
     2461 tcgagctact ctgatttgcc ttgctcgccg aatcccaaac taggaagaac aggctctggt
     2521 cgttgccgac gatatttcca atagtccctt gctgactcgc gaacagagtg gtcggcgcgg
     2581 attgcccagt ttccaacgcg cgcaattcgt ctgcatcgtt cgcccaatat actcccatcg
     2641 aatcggcaaa cacgtctttg atgtaccgct cgtccgtaac gagcttgggc ttttgaatgc
     2701 attgctgcac gggtgttgcg ccgcgctccg gctcgggtac ggcacactgc accgcgacgg
     2761 acgttggggc cgtgcagccc tccagctcgg aggttcccgg cgatcctgct tcgaccgacg
     2821 agctgccgcc agtggcgcca ccgcttcgcc ccccggactc tgagtttgcg cccgccacga
     2881 cactcatctc gcccccgaca ctgttgcctc cgacgacact gttgcctccg actcccgctc
     2941 gagcgcccga accgctggca cccgtacccg tcgcaccggt gccagctctg ccgcccgacg
     3001 aattctttgc gcccgtgccc gcgccgggcg ccgtgccatc cgcaccgcca tcggttgcgc
     3061 cgcctgtcat ttgatcactt ggctcaccac tgggcgctcg tgacgagtga gttttgccgc
     3121 agcccaatgc gacacccgaa acgcagagcg ccaatgacag cgcgccccag cgcaatcgct
     3181 tcacgcgcgc ctccaatccc aagcaatgca gccctgagcc gtttcgagga acgatcccac
     3241 aaacatcccg atgaacaacc atgccgccgt gcaaactagc agcttggtag ctcgatggga
     3301 agggccaact tggttcactc agcacgccgc acagatcaag gactgagccc tcagctcgta
     3361 ccttctcacg agcttatttc gtcacgaccg ctcgagccgt gacgttggtt cgcgcttgat
     3421 agatcgccgc acgatcgttg cggtccactg tcgccctcac tcagccccgt cactcagccc
     3481 cgtcactcag ccccatcact cagcactgcc tcgttcatcg cggccatgca cgtgcgccgg
     3541 gggtgccgtc gcttccacgg ctgaaaaggg ctcaaggatg cggtaacgat gggccgctcc
     3601 gcgcggcact acgtatgagt ccccggtttt gagcaccact ttctgttctt cgatctcgag
     3661 ctccgcgcgg ccctgcagca cgtaacccac cacctcgtag tcgcggcgaa cggttttggt
     3721 tcgcgccgtt tcatcagctt gcgtggctac ttcggattgc gcagtaacgg gttcgtcgcg
     3781 ccacagccgc atcccaagct gaaccccaga ggccaaatag aactgtccca ttgctccgcg
     3841 tggtgaatga gctgagtcaa ctttggtaac gcttgtgtct gccatgggat ctcctcgctc
     3901 gaaattctat cgagcctaac aacctcaata gcgagcaccg ttcgtgccaa cggcgcacca
     3961 agtcgagatg cggctcgagc cagcgcgttg ttcgcacgga tcagagctga atgagctacc
     4021 cgtggcacac gctttgctct tcaccggtgg gggagtccgt aatgatggag acgaaaccat
     4081 ggtcactcga gcccgagact ccgcaacgcg ccgcgatgac atggtggtga ggcaactcgc
     4141 cgctcgagac atcacggatc cacgggtatt acaagccttc cgcgatgtgc cacgtgaggc
     4201 cttcgtgccc aagtcgctcg cgcatgaggc gtacgaggac cgtccattgc ccatcgggca
     4261 acaccaaacc atctcgcaac cgtacattgt cgcgctcacc ctacaagcgt tgaatcttac
     4321 tggctcggag cgggtcctcg aaatcggcac cggatccggc tactctgcgg ccctgctctc
     4381 gcgcttggct cgtgaggtgt actcggtcga gcgcttgccc agcatggccg aaagcgcgcg
     4441 ggggaggctc gcgtcgctcg gatatcgtgt tcacgtgaag gttggagatg gcacgctggg
     4501 ctggcccgag caggccccgt acgaagcgat cgcggtcgcc gccggcggcc ccagagtgcc
     4561 cgaggcgcta gtcgcccaat tggcaagtgg cggtcggctc gtgattccgg taggcggcga
     4621 cgaaggccaa gtccttttac gggtgcttca gattccaggc gacggactgc aacacgagcc
     4681 catcgaacac gtacgcttcg tccccttgat aggggtacag ggccaacccg aacgaactgc
     4741 gtcccgagcc tgacgcggcc cgcgaggaac gggcaactcc ggctggccaa ggagcgcgtg
     4801 actactcggg cgaggctggt tccgacgagg ccaaggctgg cagtggtccc gtcaacgcgt
     4861 tgagccaagc caccaccgcg gtcacctccc ggtcactcag gcgcaagccg agttggtgtt
     4921 tgcccatcag tcgcaccgcg tcttctaggt tcgcgaccga tccgtcgtgg aagtaggggg
     4981 cggtctgggc aacgttccgc aatgacggca ccttgaacat catgcgatct acgtcttgat
     5041 gggtgacttc gaagcgacct tgatcggact ggttgggcca agcctcgacg acgccgacct
     5101 tttgatacat gccgccaccg acgaactcgc ccgtgtgaca caccatgcag cccacgttcg
     5161 tgaaggtgcg caacccttcc agttcgagct ccgtcagcgc cgattcgctg ccacgcaagt
     5221 actcgtccca acgtgaaggc gtcatcagcc gcctctcgaa cgcgccgatc gcccggccca
     5281 cgttctcgaa cgtaacgggg tccttctcgg tcgggaatgc ggcttggaag cgtcgcacgt
     5341 attcgggaat cgacttgagc agcttcacgc ctgcttctgc gctcggcaaa cccatctcga
     5401 cgggattcag gatgggtccc gtcgcttgtt cttccacggt ttgagcgcgg ccatcccaaa
     5461 actgcgcgaa gaatccggcg gcgttataga cgctcggggc gttgcgcttg ccgagctgcc
     5521 cgtccgtgcc gagcgaggtc gcgcgctggt cggcgccata cttgcccagt gggtggcagc
     5581 tgttgcacga aaggtcgccg tgcttggaca gccgcttctc gaagtagagg agtttaccga
     5641 gctctacctt atcgtccgtg attgggtttt gggggtgctc gatctgggca cgcaagggct
     5701 cgaagcgccg caagagcctc gggttgatgg cgacctgtcg cccaccctcc gtcgatgtct
     5761 cgggcggtgc cgcgtgctcc ttcaaagcgg cgagcagcag cgccgtgtca tcctgctgcg
     5821 cacaactcgc ggcgagcaag gaaagcacga gcgcggtcac ggaccgcttg ccaattggct
     5881 cagtcatagt gcctcggttc gagctgcgtc aggctggtga ggctcgcggc gtcgaagtcg
     5941 aagcggaaag acgctcctcg tccccggcta ctcgcgatcg agatcttgca gcgcttgcgt
     6001 tggcagacgt cgcgcacggc actcaagccg acgccgcgcc ccgccgcttc cgtcgtcgca
     6061 tccgcggtgg agaggccgtc ggtgaacaat gctcgttcca gatcctcggc ggtggtgtgg
     6121 ggaaacccct gttccgcagc gcgtttgcgc acgcgctccc agtcgatccc agggccgaag
     6181 tcccgcagct cgatgaagaa gctgccccgc tccagccctg cgctgagcgc aaattccgct
     6241 gctttgctct tgccgagttc gcgacgatcc gcttcggcct ccgtgccgtg cacggcggcg
     6301 ttgcggatta cgtgcgagaa cacgccccaa aactcggcaa gctcgtcgcg gtccaggtac
     6361 aactgcgggg cgtccacccg cacgctagcc gtgcctttgc ccgcccgcac cacgctcgcc
     6421 gcgaggtgct ccgcaacacg attcaagcgc tcactgatgg gctcgagccg ccacacggcc
     6481 aacatcttct ctaccgtgct gtagtcgacg cggcgctgca cggcgctcag cacgcctaga
     6541 tattcgcgct ccgccacttc gacgcgcccc tcgacgcgac tgccgagcat ctgctcgacc
     6601 ttcgtgtgaa tatgggtcca cgcacgctcg agcggggcgc aatcgagctc ggcgggagat
     6661 acactctcca aacacgactc gagatcatga caaattcggg cgatgccggt catgccatag
     6721 agcgcggtat tgcccttgaa ggtatgcaag tctcgcttga gagcaacttc gtcggtctgg
     6781 ttcgcgaaga tacgctgcga gagcgcgctg ttctctttga agaactccac gaagccttgc
     6841 cgatccgaga gcaagcgcga cgtcacggcc atcgtgtcgc gctcctcttg ttctcgggac
     6901 tcctgcgctc ggcgcgcggt cacgtcagtc atcacgacca gcagccgcgc tgcttcttga
     6961 gcatcagcca gcaacggctt gtattcgagc tccagcgtgc gcgagtcgac ttcgatcgag
     7021 cgcggcatct ggtgaatcgt gagctccagc ggcaggatcc cctccaccag ctgttgccat
     7081 cccatctcga agctctcgcg ctgggcagtg tcacgcccgg acatgtagct ccaaagcatc
     7141 gtgtcgggag ccattggtcc gaaccagcgc tcgaccgcat tggagtactc gcccacgaca
     7201 tgcgcgttct ggtcaattgt tacgaaaccc tgatcgaccg agtccaacac cagccgcagg
     7261 tcccgattct tccgatccaa ggctgccgtt cgttcgcgca ccaagcgatc caaccggtgg
     7321 ctctcctcac gcgctgactc gcgaatctga gtgatgagcg agttcaggcg gctaaccatc
     7381 gtgttgaagc tctgcccgag caaagacagt tcatcttgcg atccgtcgtc gcgcacatgc
     7441 tgggagaggt ccccgccggc tacgcgcgta gccgcttccg cgatgcgctc gatccgcctg
     7501 gcgagcgatc cggcgattag ccacgccagc gtcgttccaa ccgcaagcgc cactagcccc
     7561 gccgtgagcg ccagccagac gaggcgatcc cgtgcttgcg tcaccccgcg cagggagagc
     7621 tcgatcacca ccgtaccgcg aggaccttct aggcttcgaa cgggcgccgt agctagtacg
     7681 cgccccggca gcgagaatac gcgcggcccc ccgaaacccc gtcgcgctgc gtgcgccaga
     7741 gcgctcagct gccccgctcg atagagctcc cgcccttggc tggtatacac cgcgatgccg
     7801 tcaaccgatg cgtcccgtgc gatggattcc agcacctcac gagcggtctc ttggtcctcg
     7861 aaggccaccg cagagcgcaa ttgatgactg gaaagaccag cgtaaacccc ggcccgctca
     7921 attacctcat tgtgctgttg tttcagctga cgactaggga agtaggcggc cagccccacc
     7981 actagtagcg atatcatcgc cgcaatcaac accaccagct tggtgcgaat tcttcggctc
     8041 ggaaccagca catcgacggt cgcgggggga gccggaatgg gcacggatat cagccgcggt
     8101 tcttcgtcga cgcgacgcag cggaggtgcc ggggtcgacg ggcgagttct aggcggctcg
     8161 atttggcccg attgcattcc catcatgcaa gcggactgcg tggcacgaac gttaagctct
     8221 cgcggctcag gcggagcgat ccagtacctc cctcaccttt cgaccaaaag cctcgggtgt
     8281 gatcggcttc tgaagaaaag ctacgttggg atcgaccacc ccactcttga ccatgaggtc
     8341 gtcgggataa ccagacatat agagcacctt gatctgcgga caaacggcca gcacgcgctc
     8401 cgctagctcc gggccgctca tcttgggcat tacgatgtcg gtaatgagca gctggagttt
     8461 gcccgcgtgg agcgcacaca attcgagcgc ttcacctcct ccgtgggctt catacacctc
     8521 gtaccccgtt ctacgtaaga ccttggtggc aagcgccagc acctgctcct cgtcgtccac
     8581 cacgagtacc gtctccgtac cctggcgacg cgaaatcggg gtaagcgacg gccctgaggg
     8641 gctagaggtg tgcggctcgt cacaacgagg gaagaaaagg tgaaaggtag tccctttgcc
     8701 cagcgcactg accacatcca catgcccccc gctttgcttc attactccaa ataccgccgc
     8761 taaccccaag cccgcggcgc tgcctcgcga tttcgtactg aagaacggct cgaatacgcg
     8821 gctccgggtg gcctcatcca tcccgactcc cgtatccgag atggtgagca ccgcgtgcgg
     8881 gccattcgtc aagaggccgc tcacgccctc caactgaacc gtatccatgt tccgagtcga
     8941 gatcgtcaac ttgccaccgt tcggcatcgc gtcccgagcg ttcatgacca agttgacgat
     9001 gacttgctcg acctgtcgag agtccagccg agtgagaccg agttcagacg agagaaccag
     9061 ctcgagttcc acgtcgtccc cgaggatccg ggacagcacc ggctcgaagt gagtcaggat
     9121 gtcgttcagg ctcgatactt gaggctggag cacctgcctt ctaccgaagg tcaacaggcg
     9181 tcgcgtcaac tctgccgcct tgagcccggc gacgtgcacg gcgtttacgt cgctgcgcag
     9241 ccgctcgggc agcgcggaat cctcgagtaa cagcgaactg tagctgagga tgaccgagag
     9301 cagattgttg aagtcgtgcg ctactccgct agcgacttga cccagcgcct ccaacttttg
     9361 cgcttgccga agctgccgct ccagcgagcg cacctcggtc atgtcacgca ccagcgccac
     9421 gaaagcgcgc tctcctccgg tgagcgtcgg ctccaccgcg acactaaccg gaaactcgac
     9481 gccgtcttct cgcaccccta cccagctgag ttcttcgtgc atgacgggcg aacgcgtcac
     9541 tttgaactcc gctgcggctt tggcctcggc gcccttgacc tcgacgcctt tggcgtgcag
     9601 caacgtagag agatcgagtt cgcgggcctg agatcggatc aaatcgcgaa gcttgactcg
     9661 agtgacgcgc cgctcgccaa agaatcgctc cgcggctcga ttggccatca ggatcttgcc
     9721 atcaccggcc accagcagca cggcgtcggg caacgcggtg tgcagctccg ctagtgtttc
     9781 ctggctctgg cgcagcgatt gttcactgag cctgcgccgc gtgatgtcgc acgagaacac
     9841 gcacattccg tcgggaatgg gcacgaatcg cagctcatag aagccgcggg caccgttcgg
     9901 gtgcacgaac tcgttctcga actggtgatg agcacgcgag gccatgcagc gctgcagctc
     9961 agcgaacatt tgcgtatgct cgatacctgg gtagcactcc atcatcgtgc gccccagcag
    10021 ttcctcccgc ccgcgcccga tctgagcctc gagcgccgga tttatgtaca cataacgcca
    10081 ctccgagtcg atgatttggc agccttcgag caggttgtcc aagacgtgct gttcgacttg
    10141 cgcgctcgtg cccttcgcga cggtcattcc ttcctccctg ccggcattcc atcctccctg
    10201 cgagctctgc tgagtacgag agctttcgaa atcgagcgcg gctccttcac cgggagcact
    10261 gttgatctac gcacggaccg cagcgctcgg ctttggctct gcgctgcggc cgttacttac
    10321 gatatcaatc gaagctgcgc ttccccaaat gtttttaggc ctttgcctgg gagcagattg
    10381 tatgcaccac gtcggttaga cacctcacat gtatccgcac ccataaccga caaacacatg
    10441 acactctgac ccgccaaatc gaagccccgg gcgccgcgga cgggcgctca cggaagaaag
    10501 tcttggcccg tgagatgcgc gcggtcgttc caagtcgcaa tgaccggcga gtcctccacg
    10561 ggatcatggc tcaggatgct gattgatcca ggcgacagca tgaagtttcg ccccgcctca
    10621 ggcaccagtc ccaggaatct cgccgcgagc acacgcaaca tcacgctgtg tgcgaacaat
    10681 gccaccttac cccggagtgg cgcgagctca tggagcagcg cgtccgcacg gcgtccgaga
    10741 tcctcggcgg attctccctc ggggcaacct tccctcaaat aagtccatcc agggctttct
    10801 cgccgaatct cgtttacggt gagcccctcg tatcggccga agttcatttc caccagacga
    10861 ccttcgagct ccacattttc cattcccgcg agttctgcgg tgcggcgagc ccgttgtagc
    10921 gggctgacca gcacacgtgt gaagggcacc gccttgaggc gctcacccag ttgcacggcg
    10981 ttgcgctccc cctgctcgat gagcggcaga tccgtccgcc cagctagacg ccgctccgcg
    11041 gaccaagcgg tgtcaccgtg acggcagaga taaatggtga ggtccggcac cgggatagca
    11101 cctcaggagc gttcgatgaa aaacggcaac tccgccgagt ctgcttgtat gagtccgtcg
    11161 cgctgaatcc ttcgactatg cgccggctca gcgcggacgt ccatatcccc gacccgggtg
    11221 aaaacgacct gcgtcgtcgc gcagtgcgaa attcgacact ccccagttga ccccatgccg
    11281 gacgcacctg ctgcgggact gctgctgggc aaactgttgc tgggcaaact gctgctggca
    11341 gattgccgct gggcagactg cggcccgcag gccaccgcac gaacgcgctc tgccgccact
    11401 agccggccac gctgcgtgat agaccagttc tcagctgtgc agcttccgaa cgttttgcct
    11461 accgaaggtc gagcggacac aatcttcgca cttgagaact cagcactcgt aacccgcgta
    11521 gctgatgcta gcgcaagctc ggacaagttt ttggcaggaa ggattggcac tgccccttgc
    11581 aagcgcgggg cgggttgggt gtggccgtga agcttggcct ggcgaacaag gctagtctgt
    11641 ggctcacgtg gtgcgtggcg ttgggctgtg tgcgggaacg aagcgcgata actccctcgc
    11701 tcgaatctcg cagtcgagca aacccgtcaa tcacggcccc tagcgtcaac tccccgaacg
    11761 cgagctcccc tgtcacggga gcactttcca cgaccagcgc ctctggaccc cctattcctt
    11821 ctgtagccgt ggtgccgccc aacgatgacc aggcgccgta ccttctggtg cacgcagccc
    11881 gccgctactt catcgggtta cctctggtct tcaacgtggt gttcgagaac cgttccgcga
    11941 ttgcccactt cttgcgtttg ccgaggctcg atctactaac acgccaggcc aatcaattcg
    12001 ccgtcactct agagaggatc gataaaagcg aacccgcgag atatgtccgc ttcccgcccg
    12061 tcgaggaaga cggccccgtc gcgcagcttg ggccgatgga acgcctgagc gtaatctgcg
    12121 atctatctaa cttagcggtg acctttaccc ccggtattta ccgaatcact ttctcggtcg
    12181 ggcgcggccc caccgaacgg agctccgcac caatcgaagt cgcgctcgaa gcactcgccc
    12241 caactgacgc gcgagtagtg gggcgcttgc tgcaaccaaa cccacggggc cgcgccgaca
    12301 tcggcgcctg ggcatctttc ctcgaacacc actcgagacc gatcgggctg cccctaagcc
    12361 tcagccagga cgtgcgcaag caattggctc ttcatctgtt tctcagtgcg gccgtgcacg
    12421 gccgcgctcc catctctgag ctcgatgcgg acgcgctcgg agccattact gatcgtgctt
    12481 ggctgagtga ggc
//
LOCUS       DASTKU010000729        20619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1928640, whole genome shotgun sequence.
ACCESSION   DASTKU010000729 DASTKU010000000
VERSION     DASTKU010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 20619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20619
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928640"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VFQ61_25495"
     CDS             <1..956
                     /locus_tag="VFQ61_25495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9957888.1"
                     /translation="LLHATIESKAHLVLELGDELPPVGADRFQLRQVMTNLILNALDA
                     MEGKRGTLTLRTEATRLEGASSERYGVAAGTYVKVTVTDTGVGILPEARERLFEPFFS
                     TKGAGRGMGLAAAAGIVRAHRGWLGVDETSEHGTRFCILLPVAQKSMSRPSSTPTRLA
                     AAPPARRILLIDDEPAVRLVTGRMLSELGHHVMTADSGRRGLEILEEQADAIDLVILD
                     LTMPEQSGEQTLHQLREVRSNIAVVITSGFHAEDASMLLQIPNVIGFLDKPHTMTSLE
                     MVLASVPRHAPATQATGTQATGTQATVAQATGATKPKIAVH"
     gene            complement(1040..1414)
                     /locus_tag="VFQ61_25500"
     CDS             complement(1040..1414)
                     /locus_tag="VFQ61_25500"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9957889.1"
                     /translation="MRTLDYLLLAVRNPRESAQLYTRLLGCPPVENSETFVLYVTTTG
                     LKVGLWKASDIQPKAAPPGGIEIAFSEHSRDAVLKTYEEWKNLGLEVLQEPTDMDFGF
                     TFVVADPDGHRLRPFVLAERPR"
     gene            complement(1509..2195)
                     /locus_tag="VFQ61_25505"
     CDS             complement(1509..2195)
                     /locus_tag="VFQ61_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HET9957890.1"
                     /translation="MSRASRLLELMDLLRRRRAPISGTDLASALGISVRTLYRDIATL
                     QAQGAEIQGEPGVGYVLQPGFTLPPLMFSAEELEALVLGSRWVAVRGDARLSAAAENA
                     IAKIRAVLPRELRESVDEATLTVPSADAPPTASIDVSVVRAAVRQERKLVIGYQDGSG
                     QVTRRVIWPILIGFFERVLVVAAWCELRQDYRGFRVDRIREVEETGTPYPGKRVALVR
                     DWRERQGISR"
     gene            2574..3167
                     /locus_tag="VFQ61_25510"
     CDS             2574..3167
                     /locus_tag="VFQ61_25510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957891.1"
                     /translation="MSVEAWCVYVAGLVLALACDVGDDGSSTAGVGGQTGSGGAIGES
                     GGAPSCDGSKLPPLPNGCTPDCATEPEDQEPIVCQDGEWQCPEGRFLASECELKTCAR
                     RYENRCCDRATGNFVTPECGPSGKLEACAPGSEEWPANRDCVAADLDVSDCTELQNLA
                     CQIEGQACQPAFRCSTGCWCAKSSAGGLRWSCSSLPC"
     gene            3313..4350
                     /locus_tag="VFQ61_25515"
     CDS             3313..4350
                     /locus_tag="VFQ61_25515"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9957892.1"
                     /translation="MRATIMYAAGDVRVVTVPDPSITEPTDAIVRVVRACICGSDLWP
                     YHDMARSEAGQSMGHEAIGVVEAVGSAVRVVKRGQVVIMPFAISDGSCAFCQEGLTTA
                     CVHGGFFGYDGTNGAQAEALRIPFADGTLFPIDVTESDARMPGLLTLSDVLGTGHHAA
                     VVARVAPGHRVVVVGDGAVGLCGVIAAKRLGAEQIILMGRHPDRIALAREFGATDIVP
                     ERGEEGIARVRELTEGQGAHSVLECVGTDAATSTCVGVVRPGGAIGRVGVPQYSAIPG
                     GDTTFFKNVIVGGGPAPVRSYIRELLPEVLEGKIQPGRVFDQVVGLDGVPEGYRAMDQ
                     RKSIKVLVIPS"
     gene            4481..5941
                     /locus_tag="VFQ61_25520"
     CDS             4481..5941
                     /locus_tag="VFQ61_25520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957893.1"
                     /translation="MGTAVEKLAATLSLPRLASTGRAARGLLNGTLEGSLRKRSLVAS
                     GLALFVTVLLYLLPDVHPLLQNADSAVYNDQVDARNLQSRTTHWGYMMLGVFARTLLP
                     GPTDSVMNFMTLSAGALGIVACFTAAHRATNALIPSVIASLTLLTVPAYLRGMVLSEV
                     DVPLTVFLSASFALALLRKPVFSGLSFGAALVISPLAGLTLAGILVVIVPEADGKLRV
                     RGALKRLTLFGVASFAMYAPVVLANWQDYVYGGRGILHASRKNFDVVTRVHRSAEFLY
                     RQCGPLLLLWGLGFIVGLRRRRYVIVFAPVVGLLVATLLGERLIDVPVQLPTIALMVP
                     LCALALGALWRGRGQIVVSAIACSVVLAGIWRGFESTHSLLEAQMARMIAERDAYIAL
                     AESSRRAPLLVNIKGYDQGRRVERMVFGSAHPEHALSRKAFQRRCKRLASARGHFIWF
                     VASPHRLPCAELEASYQVQSRTLNGRRFKVLIPRTG"
     gene            complement(5987..6535)
                     /locus_tag="VFQ61_25525"
     CDS             complement(5987..6535)
                     /locus_tag="VFQ61_25525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9957894.1"
                     /translation="MLPARVALSFAHVYARFFDFVWAATRRMGIDPESIDDVVQEIFI
                     VIHQRLHTLERPEAIRSWVYGITRRTVSTYHRTVRNKKAGADTLGLSVEMLETQVPSP
                     LDLIEQNAQVQLLQSLLRELSPTKREVFVLAEIEELSVPEIAEALEIPLNTAYSRLRA
                     SRQDFEAALARYTMRSAREEAS"
     gene            complement(6735..7556)
                     /locus_tag="VFQ61_25530"
     CDS             complement(6735..7556)
                     /locus_tag="VFQ61_25530"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9957895.1"
                     /translation="MADRPTLYQRFLPEGGQHSFVWKYSDPIGGRRPRHFHGEPEVNL
                     VVRGSATFGVGDRIAEVSQGELLVFPSGQDHVLIQASPDLYLYAIGLDLPYSAQVLGV
                     RNQPVVPLHVRIAPHELRGVLNRAASIVDRAGTAQLGAELWEHIHWLAGRAADQTRCR
                     AHVLTRRSLQRMTESPELGLDALADELRAQASEISRNFHQDLGMTLVRYRSRQRLLHA
                     IRLIDNAGHDLMAVASIAGFGSYSQFHRTFHAELGCSPRQFVSGVRKQMQLAYED"
     gene            7736..9046
                     /locus_tag="VFQ61_25535"
     CDS             7736..9046
                     /locus_tag="VFQ61_25535"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET9957896.1"
                     /translation="MRRIPLVPLCLLATLLLPRAALAVRGSELYTSKAYGYGRFEARV
                     RFAAGDGVVSSFFLWKDGSEKSGTFWNELDFEKLGADCHVETNAIYGNPAGNHSQRNG
                     TATDFCTAYHTYAYEWTPSAIAWFIDGVEVRRETGAVAAAFADNAAGGMQIHFNVWPG
                     DASFGGNFSPNILPVHQYVDWVQFSAFANGNFTLSWREEFDGSTVPSGWLTGDWASPK
                     NLSTHDPRNVNLIRSNVVLSLTADNAVGPAGAMPNSSGGTAGTGAGGASGGSATGGST
                     MTAGGSGGSQGGAGNAGNPSQGGVAGMAGMPSSGGTTASGGVSAGGASPGVSGAPSGG
                     SAGAIVSGGASSAAGSGNNTAGSSTAAGASGGLAGSGAGAGQGGLAGVSGAATTPNPP
                     GTPPGKDNGCNIATPSSGSPLGAFGLFAIAALGLGRRRRARATR"
     gene            9094..10038
                     /locus_tag="VFQ61_25540"
     CDS             9094..10038
                     /locus_tag="VFQ61_25540"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET9957897.1"
                     /translation="MFRTALPTLCFVAVALVPQVAAAVTSAELYTGESYRYGRVEARV
                     RFAAGDGVVSSFFLWKDGSEQTGNYWNELDYEKLGADCHLETNAFYGKPAVTHSQRHS
                     LNIDLCGGYHTYVYEWTPEAIVWTIDGMEIRRETGAAAAAYADNAAAGMQIRFNIWPG
                     NASFGGNFSPSILPVHEYVDWVQYSSYANGAFTLEWRDDFDAPQLASRWLTGSWASPK
                     NLSTHDARNVNIVNGYAVLSLTADDAVGPAGAAPEAVNNGTAGGPSVPVTNTSNDGGC
                     SVGSPRSGSFGTWLLACFAGIGVISRRLRRRSLEATRS"
     gene            complement(10065..10805)
                     /locus_tag="VFQ61_25545"
     CDS             complement(10065..10805)
                     /locus_tag="VFQ61_25545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957898.1"
                     /translation="MPPASQPDSSIRDRLTLLDDYVQSRDYPRNVCHLLGSLGRRLGF
                     PEAPDEIGVVCPNVLEASEYLTHQYGAGPFFLGSGSPKDFTEAGVVKPYTTRIGFGYL
                     RGVLIELAEPGLGSNLFGRALDPRGRITLHHLGFFARGASLCRKDIRGRRAHFDDRML
                     GAGYRPGVTTRLSALGLVGNVTIFETGPETHGIGTEFLDIRLFGRRGIPFGLPQWLLE
                     LGARFEIRSGHRLVHLPADHELPPAPKS"
     gene            complement(10888..11733)
                     /locus_tag="VFQ61_25550"
     CDS             complement(10888..11733)
                     /locus_tag="VFQ61_25550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HET9957899.1"
                     /translation="MLKERERTSTLQRVFALLADALRWSLHDRVSRVLVSSFVLIMLW
                     GTHGRVDLLTLVLDGWTGPGGDPHTRARLIAGLKWDQEWIAFGIGLLLLVVTPCLLIR
                     FWFRESPLDYGLGLPKPGAWPFTLVSTLVLGVFAVPSMYFASRDAGMRATYPFYREFA
                     GLGEFARYEAGYLLFFVTIEFTFRGYLLFGLYRIQDSMTASAPSTDMGARGPLLFGRY
                     SVLVSMLSYTAWHLGKPLPEQYGTLIWGLITGTLALRSGTIWHIVIVHFAMNVWLDYL
                     IYVSL"
     gene            complement(11727..13457)
                     /locus_tag="VFQ61_25555"
     CDS             complement(11727..13457)
                     /locus_tag="VFQ61_25555"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9957900.1"
                     /translation="MHGPTASSDREGVCGRLIRFGATLLALVGHVVLALLITAQQLWQ
                     VASRYPQALVRVWLPRPLPGSIDVTHAGHFENVTKNQFARPATIRSITEGEQIARVIR
                     EAEAATPPRRVHAVGSGHSFSDAAIADDVLLELKDWNTLLPLDALSLRSGVDRGQLVR
                     AASGISIRALNQALKRLGLALPNMGGYDGQTLAGAISTGTHGSGRTLGPLSDMVRSLD
                     LITEGGRALRIEPCGGPSEPCSHATLCPDIELHQDDALFHSVVVSMGCMGVVHSYVLE
                     VCPEYYLGEHREVLEWQEVRRKLVQQEFQPTLHSLRDKPIRHFEFLVSPYERRGKRQC
                     LVTYRWPEKEAAKTRPDRTRAFVATLFTSIREFDEIYALALTVWPRLSTWVIEREIQS
                     LSDGLYVDESFRVLHLGDANYVPAYGSELAFALNEPDPATGLPQYVAALERLFQLARE
                     QASRGRYHNVPVSVRFVAESPHLLSLSHGRASAIVELPMLARVPGGWDLLRFYERVLF
                     NEFGARAHWGQANFSVGADRICKSYGAAFVTWLEQHAKLCPRGTFQNSFSERMGVRAL
                     GRAIRGQSIC"
     gene            complement(13461..15584)
                     /locus_tag="VFQ61_25560"
     CDS             complement(13461..15584)
                     /locus_tag="VFQ61_25560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957901.1"
                     /translation="MSSKWRDIPHEGDPAFIATESGGRPRKPSEILGPVSWGFGRNFL
                     GGLTRTLAFDWFPDRLDPRDWMSAESVTTQPAQPSMARADAREFWFDYISDMGDSFRA
                     TYSAYYLLHGDLFVDDPRLLSADEPTLTPPGRVREWEIQYRGDDFALRLPTGAFLFCG
                     GDAAYSVADEKTLRARLQTPCDLAHAARRPGNQTSTRSSRPLYGIPANHDWYDSLDGY
                     NRLFRIPPSGAAPVIQLAGFTSVQQASYVAIDLPFDWCIWGIDSRDSTDVDFRQKQFF
                     KRHAEIPKRLVVVTSKPLVVYNETQSWAAQLLAELGLELEPRPPQARLYVSGDSHHYA
                     RYGLGSGAFSIVSGLGGATLHPPPHLAGHATQSCDAFPHPKATRRTITSRLANPVHML
                     LRSGVTGIGAVVGAVVGASLCSYEGELSRSLVRAGWLTLGAPRLPDGVAWVFWAAMLL
                     LFTVGAWFGVGHRLFQFDRSDGEQRARRGWSKVARGFGLVAVTFVFSSLAILVAVWLG
                     GGRSTPGVTLDLLVYVILITLGPGLGPIALLQSHNRQQLHPARQAASLVLGTLSGCAM
                     VGALLFVAARLASALAPHEPLHSLIQSSLPPTPRGVLAYVLLTALGALLGGVVGAIVI
                     PVLFGWAMLIQYALGAHYSLPGTFALLDDYVCFIRFRVRELASGATDLTGFVIGARSV
                     ARGELMAGRTPAPRAHLIDVFRIRG"
     gene            15698..17944
                     /locus_tag="VFQ61_25565"
     CDS             15698..17944
                     /locus_tag="VFQ61_25565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957902.1"
                     /translation="MPGAGVRGKGSKLVERTLPCGPTLRVGHALAVGAVVLLGTSRGD
                     AAIDDFLRGVGRTLGAPFGGFVESLVTPSLQAGEQTGKELVADVDRRVGERIHQIGVT
                     GGALLSRADSLAASRIGQADGAVAARLTQADAMLTARITQLDVGANRTLDRALGRLDG
                     SVQSAVRGLGAEARSAVRQLDQAGKARLEQADAILESRIDQLGAVVNQSIVDLDQNLE
                     NRLEQVDDISERRVGNLDVVGTRQVLGAESALTRLAVLAAVLGLIVIFLKSLGAEIWE
                     RWEETEREQARWAGRVRSMLSPSLFLGGARVLLAHLAIAGVCVGVVLLVAKRLPRETE
                     QRSQQLLATHQAGFEESIQSIDHRRALYFASQLAIIDPLRSGEYTRRAEKIRLVRDVL
                     VRPGLFQRPDAVRETARRIAVLSAELHDTDGDLLTLQCYVRWQLARNREDEAAASADC
                     QRALALPDHSPFRLRPLAQHYLAALATFPSWRLAELPSPPVFEPPSALLPGLEHIFTY
                     AALVRKLDEDATRAYLQMLAAESDLHKALERLPSKPKALPPRAPLSPEQKTFEDSVAR
                     RLAHAEQVVEAWRAFDDALQHEPWLAGTNTELAAFLLDDAVLTRALMVVARPREIHLG
                     LRLAELPESLHRIEAAPLRIEWMRRYFGGLGESVARIVSYEESRRFLDHERELVEFEK
                     AYVAFHLSESPNAQLRFSLALSSARVGLYVSEHGRRRPFAQTLGLVEAAQISEVQRAA
                     DERHVRLL"
     gene            18381..19040
                     /locus_tag="VFQ61_25570"
     CDS             18381..19040
                     /locus_tag="VFQ61_25570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957903.1"
                     /translation="MLLTVACGGSSDNELNAMGGSTSGTSGGTANATGGVTNSNGGTT
                     SVSGGSQSGGASATSGGASSTSGGASSTSGGATTKDPFSTPVTCTSNVKWTRGENDRM
                     RPGEACIACHAQTGDAPKFTIAGTVYPTGHEPNDCNGVTAATATTIVITDANQVEHKI
                     SANSAGNFMLSTSVATPYTAKVVTSVGERKMFSPQTSGDCNSCHTQDGSNGAPGRVLI
                     P"
     gene            19227..19814
                     /locus_tag="VFQ61_25575"
     CDS             19227..19814
                     /locus_tag="VFQ61_25575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957904.1"
                     /translation="MTPISDTGFAQLESEGRLLNPVFKGPTHVPGRFGFRGELALKFA
                     SKQADEARPPELMCQQVLAIAERQPTIPFFAGYLLSFAELKPLAEALGKTISAGGKYF
                     VFCNNIDLGSRYQVPYAGAMWYVLPIDEATVWNEMLDLVHLDRNDLKKLDTAAKLDRL
                     AQAAIQYDVTFDMMTYAEGLERMGPVRNPNENRPV"
     gene            complement(19946..>20619)
                     /locus_tag="VFQ61_25580"
     CDS             complement(19946..>20619)
                     /locus_tag="VFQ61_25580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957905.1"
                     /translation="IASESLGYGATATWDGSSYWLAWETENHHVMGRNLAEDGTLGEI
                     STWIGEGDCAGVTLTSDRQGQLLLSYVRYTNMGNARRVHSRLVGRGADTVPNGGTGGA
                     GGGTSTNPGTSGTGASAGSSAAGASAGGTTTTQSTGTGANSTGGTTAASGGAAGSSSS
                     SSGSTSRGGTTSRGGSTGTGGSSNPGFTCSVGNIGGGSTSTGAIAGLGLAVTLAAVRR
                     RRRVV"
BASE COUNT         3832 a         6374 c         6666 g         3747 t
ORIGIN      
        1 gtctgctgca cgcaaccatc gaatccaagg cccacctggt cctggagctc ggggacgagc
       61 tgccgccggt tggcgcggat cggtttcagt tgcgtcaggt catgaccaac ttgatcttga
      121 acgcgctcga cgcaatggag ggaaagcggg ggaccttgac ccttcggacg gaagccacgc
      181 gtctcgaggg cgcgagcagc gagcgctacg gggttgctgc gggtacctac gtcaaggtga
      241 ccgtcaccga tacgggcgtg ggtattctgc cggaggcccg agagcgcctg ttcgagccgt
      301 tcttttcgac caaaggtgcg ggcaggggca tgggcctagc agccgcggcg ggtatcgtcc
      361 gcgcccatcg cggctggctc ggggtggatg agacttctga gcacggaacg cgcttctgca
      421 tcctgctgcc ggtcgcgcaa aagtccatgt cgcgcccctc cagcactccc accaggttgg
      481 cggcggcgcc tcccgcgcgt cgtattctgc taattgacga tgagccggcg gtgcgcctcg
      541 tgacgggcag aatgctgagc gaactcggtc atcatgtcat gaccgcggac agcggcaggc
      601 gcgggctcga aatcctcgag gagcaagccg atgccatcga cctcgtcatt ctcgatctga
      661 cgatgccaga gcaatctggc gaacaaactc tgcatcagct gcgcgaggtt cggagcaaca
      721 tcgcggtggt gatcacgagc ggatttcatg cggaagacgc ctcgatgttg ctgcagatcc
      781 ccaacgtgat tggcttcttg gacaagcctc acaccatgac cagcttggag atggtattgg
      841 cgtcggttcc tcggcatgcg cccgctaccc aagcgactgg tacccaagcg actggtaccc
      901 aagcgactgt tgcccaagcg actggtgcca ccaagcccaa gattgcggtg cattgacggc
      961 tcaattgcac cagccgcggc tgctctcgat tcccaccgag cgcgcattag gtctattcgt
     1021 ttcgaacgct cggcgcagct cagcgcggcc tctccgctag tacgaatggc cttaggcggt
     1081 ggccgtccgg atcggcaacc acgaaggtga atccgaaatc catgtccgta ggttcctgca
     1141 agacctcgag cccgaggttc ttccactctt cataggtttt cagtacggca tcacgggagt
     1201 gctccgagaa agcgatttcg atgccaccgg gcggggcggc tttgggttgg atatcgctgg
     1261 ccttccagag gccgactttc agtcccgtag tggttacgta caatacgaag gtctcggagt
     1321 tctccaccgg cgggcagccg agcaatcgag tgtagagttg tgcgctctcg cgcgggttcc
     1381 ggacggcgag caatagataa tcgagggttc gcatgatgtc tcctgttcgg tagagccatc
     1441 ttgtgggtcc gcggtgtcag tttttggcag tagcggagcc cgtactcgaa ggcgcggctc
     1501 ttctgatctc agcgcgagat cccctgccgt tctcgccaat cccgaaccag cgcgacgcgt
     1561 ttgcctgggt agggagtgcc cgtttcctcc acttctcgaa tgcggtcgac acggaatccc
     1621 cggtagtcct ggcgcaactc acaccaggcg gccacgacca gcacacgctc gaagaagccg
     1681 atcaggatgg gccagatcac ccgccgtgtg acttggccac tgccgtcttg atatccgatg
     1741 acgagctttc gttcctgccg aacggcagcc cgcaccaccg agacgtcgat gctcgcggtc
     1801 gggggtgcat cagcgctcgg caccgtcaaa gtcgcttcgt ccacgctttc ccgcaactca
     1861 cgcggcaaca ccgcgcggat cttggcgatc gcattttcgg cggcagcact gaggcgtgca
     1921 tccccacgta ccgcgaccca gcgcgagccc agcaccaacg cctcgagttc ttcggccgag
     1981 aacatcagcg gaggcaaggt gaagccgggt tgcagcacat aaccaactcc gggctcaccc
     2041 tgaatttccg cgccttgggc ctgcagcgtc gcaatgtctc ggtagagcgt gcgaaccgaa
     2101 atgccgagcg cgctcgctaa atcggtcccc gagattggag cacgccggcg tcggagcagg
     2161 tccatcagtt cgagtagccg tgaggctcgg gacatccgcg ccccgtctca tggctcggcc
     2221 ttggcgtcca ctctaggcgc gcaaactagg ccaggaagca cgaagcgagc acgagagcag
     2281 actcactcgc atcattcgcg tgctgccgag tgcgcctcga gaaggcgaac cgacgaacgg
     2341 agagcgactc tttcccacga cggctcaggc tcgcgcggct gcccgcaccg tcacgggccg
     2401 gcgcatccct tcaggctgac ggcgctcatt gtcgagcagc cgcgaggcga cggcgagcca
     2461 agctttcatc gcgcgtcctt gagcagggga cctctcggag caaactgcgg cggaacactc
     2521 gccgcgactc ggtcgtagtt gcgggccggc gttgtccgtg gaagcttcct tgcatgtctg
     2581 tcgaggcgtg gtgtgtgtac gtggctggtc tggtgctcgc gttggcgtgc gacgttgggg
     2641 acgacgggag ctctacggca ggtgtgggtg gtcaaacggg cagtggtggt gcgattggag
     2701 aatctggagg cgcgccgagc tgcgacggct cgaaattgcc acctcttccg aatgggtgta
     2761 cgccggattg cgccacagaa cccgaggacc aagagccgat cgtctgtcag gacggagagt
     2821 ggcagtgtcc cgaaggtcgc ttcttggctt ctgagtgcga gctgaagacc tgcgcccgcc
     2881 gctacgagaa tcgttgctgt gaccgggcca ccggcaactt cgtgactcca gagtgtggcc
     2941 cttcgggcaa gctcgaagcc tgcgcgccgg gttccgagga atggccagcg aatcgcgact
     3001 gcgtcgcggc tgacctcgac gtatcggact gtaccgagct ccaaaatctg gcctgtcaga
     3061 tcgagggtca ggcttgtcag cccgcgttca ggtgcagcac gggctgctgg tgtgcgaaga
     3121 gctcggccgg ggggctccgc tggtcgtgct cgtcgcttcc gtgctgaggg tcacgcgcga
     3181 cgtggcaccg atgcgctgaa cagtgcgtcg agccattcgt cgagcacacg agagctagtc
     3241 gggcgaacga gaatggtgca gtctcgagcg ctccaatcga gtcgagctct cgacccagaa
     3301 agaaggtacg acatgcgcgc aacgatcatg tacgcagccg gggacgttcg agtcgtgact
     3361 gtgccggacc cgtccatcac cgagcccacc gacgccatcg tgcgcgttgt ccgcgcctgc
     3421 atctgtggca gtgatctctg gccatatcac gatatggccc gcagcgaggc cgggcagagc
     3481 atgggtcacg aggccatcgg ggtggtcgag gcggtgggtt cagcggtccg cgtcgtgaaa
     3541 cgcggtcagg tcgtgatcat gccgttcgcc atttccgacg gcagctgcgc gttctgccaa
     3601 gagggtctaa ccacggcctg cgttcacggc ggcttctttg gctacgatgg gacgaatggc
     3661 gcgcaagccg aagcgttgcg catccctttc gcggacggga cgctattccc gatcgatgtg
     3721 accgagagtg acgcacgcat gccagggctg ctcacgctct cggacgtatt ggggaccggc
     3781 catcacgccg cggtggtcgc ccgcgtggcg ccgggtcatc gcgtggtggt cgtcggagac
     3841 ggagcggttg ggctatgtgg tgtgatcgcg gccaaacgct tgggtgcgga gcagatcatc
     3901 ttgatgggcc ggcatccgga tcgaattgcg ctcgcgcgcg agttcggggc gacggacatc
     3961 gtccctgagc ggggtgagga gggaattgcg cgtgtgcgag agctcaccga gggccaaggg
     4021 gcacactccg tgctcgagtg tgtcggcaca gacgcggcga cgagcacctg cgttggagtg
     4081 gtccgccccg gaggcgcgat cgggcgcgtc ggtgtccccc aatactcagc aatcccaggg
     4141 ggtgacacga cattcttcaa gaacgtcatc gtaggaggcg gcccggctcc cgtgcgttcg
     4201 tacatccgtg agctcttgcc ggaggtactc gagggcaaga tccaacccgg gcgtgtcttc
     4261 gatcaggtcg tgggtctgga cggcgtgccc gagggttacc gagccatgga tcaacgcaaa
     4321 tccatcaaag tcttggtcat accgtcatga ggagctgagc cggtgacttc gggtgggggg
     4381 cggctgttgc agccgcaccg gccgatccag ggggacgccg ggtgaggtcc tgcagatcga
     4441 attgaccgca cgcgagccct gaaggatggt gcgcgccgcc atgggcacgg cagtcgaaaa
     4501 actagcggcg actctgtcat tacctcgact cgcgtcgacc ggtagagctg ctcgtggtct
     4561 gctcaacggc acgctcgagg gatcgctccg caagcgttca ctggtcgctt cggggctcgc
     4621 cctgttcgtt acggtgctcc tatacctgct gccagacgtg caccccttgc tgcagaacgc
     4681 cgactctgcc gtttacaacg accaggtgga tgcgcgcaat ctacaatcga gaacgaccca
     4741 ttggggttat atgatgctgg gagtcttcgc gcggaccttg ctgcccggcc ctacggattc
     4801 agtcatgaac ttcatgactc tgagtgccgg ggcgctgggg attgtagcgt gttttacggc
     4861 ggctcatcga gcgaccaacg cattgatccc gagtgtcatc gcttcgctta cgctgctcac
     4921 ggtcccggcg tatctgcgag ggatggtgct gtccgaggtg gatgtgccgt tgacggtctt
     4981 cctgtccgcc tcttttgcgc tcgcgttgtt gcgaaagccg gtgttctctg gcctgagctt
     5041 cggcgccgcg ctcgtgatct ctccgctggc tgggcttacg ctggcgggca ttctggtggt
     5101 gattgttccc gaagcagacg gcaagcttcg cgtcagaggt gcactgaagc gcctgacatt
     5161 gtttggcgtc gcatctttcg ccatgtatgc gcccgtcgtg ctcgcgaatt ggcaggacta
     5221 tgtgtatggc gggcggggaa tcctccatgc gtctcggaag aacttcgatg tcgtgactcg
     5281 agtccatcgt tcagctgagt tcctatatcg ccaatgcggg ccactcctcc tcttgtgggg
     5341 attggggttc atcgtggggc tgcggcggcg ccgttacgtc atcgtgttcg cccccgtggt
     5401 tgggctgctg gtggccacgc tcttgggcga gaggctcatc gacgtgcccg ttcagctgcc
     5461 gacgatcgcg ctcatggttc cgctgtgcgc gctcgcgctg ggagctctct ggagaggccg
     5521 cggccaaatc gtggtttcgg cgatcgcatg tagcgtagtt ctcgccggga tctggcgagg
     5581 cttcgagtca acccactccc tgctcgaagc acagatggcc cggatgattg cggaacgcga
     5641 tgcctatatc gccctcgccg agagctctcg cagagcgcct ctgctcgtca atatcaaggg
     5701 atacgatcaa gggcgcagag tcgaacggat ggtgttcggc tcggcgcatc cggaacatgc
     5761 cttgtcccgt aaggcctttc agagacgttg caaacgtctc gcatctgcgc ggggacactt
     5821 catctggttc gtggcgtcgc cacaccggct gccgtgcgcc gagctggagg ccagctacca
     5881 agttcagtcg agaacgctga acggacgtcg attcaaagtg ctcatcccgc gaacgggctg
     5941 actctcggtt cgaaggcgcc agttcagggc gctgctcggg gcgctctcac gaggcctcct
     6001 cgcgggcgga gcgcatcgtg taccgggcga gcgcggcctc gaaatcctga cgactcgcgc
     6061 gcagccggga atacgcggta ttcagcggga tttcgagcgc ctccgcgatc tccggcacgc
     6121 tgagttcctc gatctccgcg agcacgaata cctcgcgttt cgtcgggctt agctcacgga
     6181 gcagcgattg gagcagctgg acttgagcat tctgctcaat cagatcgagc gggctcggca
     6241 cttgcgtttc cagcatctcg acgctcaacc ccagcgtgtc cgctcccgcc ttcttgttgc
     6301 gcacggtacg gtgataagtg ctcaccgttc gccgcgtgat gccatagacc cagctgcgga
     6361 tggcttcggg ccgctccagc gtgtgaagcc gctggtgaat gacgataaag atttcctgca
     6421 ccacgtcatc gatcgactcc gggtcgattc ccatccggcg agtggcggcc cacacgaaat
     6481 cgaagaagcg cgcatacacg tgcgcaaacg agagtgcaac ccgggcaggg agcatgctga
     6541 gcagctttga cgcttggcaa acaagcagac gacgccgcaa tagctcacgg ctacgtgaaa
     6601 ttgctggttg aagggcgtgt ctactcagga gggcagtggc agtcggctca cgcttctgtc
     6661 agagaaaatg cgtgcgccgc gctttcatgg cgagccactc gattcgcggc atcgggggct
     6721 tggacgccga acgttcagtc ctcgtaggcg agctgcatct gcttgcgtac gcccgaaacg
     6781 aattgacgcg gggagcaccc gagctccgcg tggaaggtcc gatgaaactg ggagtacgaa
     6841 ccgaaacccg cgatgctcgc gaccgccatc aaatcgtgcc ccgcgttgtc aatcagacgg
     6901 atggcatgga gcaggcgctg ccgcgatcta taccgaacca gcgtcatccc caaatcctgg
     6961 tgaaagttgc ggctgatctc actcgcctga gccctcagct catccgcgag ggcgtctagg
     7021 ccgagttcgg ggctttcggt catgcgctgc agcgaccgtc gggtcaggac gtgggctcgg
     7081 caccgggttt gatccgcggc gcgtccggcg agccagtgga tgtgctccca aagctcggct
     7141 ccaagctgag cggtccccgc gcggtctacg atcgacgcgg cccggttgag cactccgcgg
     7201 agctcgtgcg gcgcgatgcg gacatggagc ggcaccacgg gctggttgcg aaccccgagc
     7261 acttgcgccg agtagggaag gtccagcccg atcgcgtaga gatagaggtc cggcgaggcc
     7321 tggattaata cgtgatcctg cccggacggg aacaccagca gctcgccctg ggagacctcg
     7381 gcgatgcgat cgccgactcc gaacgtcgcc gagccccgca ccaccaggtt tacctcgggt
     7441 tcaccgtgga aatggcgtgg ccgtctgccg ccaatcgggt ccgaatactt ccacacgaag
     7501 gagtgctgtc caccctcggg aagaaagcgc tgataaaggg tcgggcgatc agccattgct
     7561 tcgggcgtct aaggtatctc ttgcgcgccc ggggcgccaa cgtagagccg cgggggcagg
     7621 cagcagaccc gggcgccggt ccgtgacgcg ttcgccgccc gtttccccaa gaaacgcgaa
     7681 ctcgcacccc gggagcggcg ctaaaccctg ggcgcagcca aggaaagcta gtcatatgcg
     7741 ccggattccg ctggttcccc tctgcttgct cgcgactctc ctgctacccc gcgccgcgct
     7801 ggccgtacgt ggcagcgaac tctatacctc caaagcctac ggttatggcc gtttcgaagc
     7861 gcgggtgcgg ttcgcggccg gcgacggcgt ggtgagctcg ttcttcctct ggaaggacgg
     7921 ctcggagaaa tctggcacgt tctggaacga gctggatttc gagaagctcg gcgcagattg
     7981 ccatgtcgag acgaacgcca tctacggcaa tcccgcgggc aatcacagcc aaagaaacgg
     8041 aacggcgacg gatttctgta ccgcgtatca cacctatgcc tacgagtgga ctcccagcgc
     8101 gatcgcctgg ttcatcgacg gcgtcgaggt tcggcgcgag acgggggcgg tagcagctgc
     8161 attcgccgac aacgccgcgg gaggcatgca aatccacttc aacgtgtggc ctggtgatgc
     8221 cagcttcggt ggcaacttca gtcccaatat cctgcctgtc catcagtacg tcgattgggt
     8281 gcagttctcc gcctttgcga acgggaattt tactctctcg tggcgtgaag agttcgacgg
     8341 ctccaccgtg ccgagtggct ggctcacggg cgattgggct tctccgaaga acctctcgac
     8401 gcacgatccc cgaaacgtga acctcattcg cagcaacgtg gtcctttcac tgaccgcgga
     8461 taatgccgtg ggcccggcag gcgcgatgcc caactcttca gggggtactg ctggtaccgg
     8521 agctgggggc gcctcgggcg gtagcgcgac gggcggctcg acgatgaccg cgggcggttc
     8581 gggcggttcc caaggcggcg cgggcaacgc cggcaacccg agtcagggtg gcgttgccgg
     8641 tatggcgggg atgccgagca gcggaggcac gaccgccagc ggcggcgtaa gcgcgggtgg
     8701 cgcttccccc ggtgtttccg gtgcaccctc ggggggctcc gcgggagcaa tcgtgagtgg
     8761 aggcgcatcg agcgcggccg gctcagggaa caacacggcc ggctctagca ctgcagcagg
     8821 cgcgtcgggt ggcttggcag gatcgggtgc cggcgcgggt cagggcggtt tggcaggcgt
     8881 gagcggcgcc gccacgactc ccaacccacc gggcacgcca ccgggcaagg acaacggttg
     8941 caacatcgcc actccgtctt cgggctcgcc gctcggcgcg ttcggtctgt tcgcgatcgc
     9001 ggcattgggc ctcgggcgac gacgacgcgc gcgcgcgacc cgatgaaacg cggctgcgac
     9061 agatagacta tgaataacga aagaatacat tgcatgttcc ggactgcact gccgacgctt
     9121 tgctttgttg cggtcgcgct ggttcctcaa gtggccgccg cggtgactag cgctgaactt
     9181 tatactggcg agtcctaccg atatggccgc gtcgaagcgc gcgttcgatt cgcggcggga
     9241 gatggggtcg tgagctcctt cttcctttgg aaagatggct ccgagcagac gggaaactac
     9301 tggaacgagc tcgactacga gaagctcggt gcggactgcc acttggagac gaacgccttc
     9361 tacggcaagc cggcggtgac tcactcccag cggcactcgc tgaacatcga tctctgtggc
     9421 ggctaccaca cctatgtcta cgaatggacc cccgaggcca tcgtgtggac catcgatggc
     9481 atggagattc gccgcgaaac cggcgccgcc gccgcggcct acgccgataa cgcggccgcg
     9541 ggcatgcaga tcagattcaa tatttggccg ggtaacgcga gcttcggagg caacttcagc
     9601 ccgagcattc tgcccgtcca tgaatacgtg gattgggttc agtactcgtc ttatgcgaac
     9661 ggcgcattca cgctggagtg gcgtgacgat ttcgatgcgc cacagctggc ttctcgttgg
     9721 ttgacgggca gctgggcctc gcccaaaaat ctgtccaccc acgatgcgcg aaacgtgaac
     9781 atcgtgaacg gttacgccgt gctctcactc accgcggatg atgcggttgg gcccgccgga
     9841 gccgcgcccg aagccgtcaa caatgggacc gccggtggac catccgtgcc ggtgacgaac
     9901 actagcaatg acggcgggtg cagcgtcggt tcgccgcgaa gcggcagctt tgggacctgg
     9961 ctattggcct gttttgccgg gatcggggtg atctcacgac ggctccggcg gcgttctctc
    10021 gaggccacgc gcagctgaag tttcacggag cggggggctc ccgctcaaga cttcggcgcc
    10081 ggtggcaact cgtgatcggc gggcaggtgc acgaggcgat gtccggatcg gatctcgaaa
    10141 cgtgcaccca gctcgagtag ccactgcggt agcccaaacg ggatgccgcg ccttccaaac
    10201 aaacggatgt cgagaaactc cgtgccgatc ccgtgcgttt ccggtccggt ctcgaagatc
    10261 gtgacattcc cgaccaagcc caacgcggac aagcgcgtgg tcacgcccgg gcggtagccc
    10321 gcacccagca tgcgatcgtc gaaatgggcg cgacgaccgc ggatgtcttt acgacagagg
    10381 ctcgcgccgc gcgcaaagaa cccgaggtga tgcagggtga tccgaccgcg cggatcgagc
    10441 gcgcgcccga acagattcga gcccagaccc ggctcggcga gctcgatcaa aaccccgcgc
    10501 agatagccga agccgatgcg cgtcgtgtaa ggtttcacca cgccggcttc ggtgaaatct
    10561 ttcgggctac cggagcccag aaaaaacggg cccgcgccgt attggtgcgt gaggtattcg
    10621 gaggcttcga gcacatttgg gcaaaccacc ccaatctcgt ccggagcttc ggggaaaccg
    10681 aggcgacggc ccagcgaccc gagtaggtgg cagacattgc gtgggtagtc gcgggactgc
    10741 acgtagtcgt ccaacagggt gagtcgatcg cgaatgctcg agtctggttg cgacgctggc
    10801 ggcataggcg gagttgctcc cgagcctaga tcttgctccg agctctaggc tgaagttgat
    10861 ttgcgccgaa ttgaacggtt ttgggcctca tagagacacg taaataaggt aatccagcca
    10921 aacattcatc gcgaagtgca cgattacgat gtgccaaatc gtccccgagc gcagcgccag
    10981 cgttcccgtt atcaaacccc agattaaggt gccatattgc tcgggcagcg gcttgccgag
    11041 gtgccaagcc gtgtaagaca gcatcgaaac caggaccgaa taccggccaa acagcaaagg
    11101 tccgcgtgcg cccatatcgg tggatggtgc tgatgccgtc atcgaatctt ggatgcggta
    11161 caagccaaac aacaagtagc cgcggaacgt aaattcgatg gttacgaaaa atagaaggta
    11221 tcccgcttcg taacgagcaa actcgcccag cccggcgaac tcccgataga aggggtaggt
    11281 tgcgcgcatg cccgcgtcgc gactcgcgaa gtacatgctc gggacggcga acacgcccaa
    11341 caccaaggtg gagacgaggg tgaaaggcca ggctcctggc ttcggcagtc ccaatccgta
    11401 atcgagggga gactcccgaa accagaaacg aatcagcaag cacggcgtca ccaccagtaa
    11461 gagcagaccg attccgaagg cgatccactc ctggtcccac ttcaaacctg caatcaatct
    11521 cgctcgcgtg tgcgggtcac cgccggggcc ggtccagccg tcgagcacca gcgtcagcag
    11581 atcgacgcgg ccgtgcgtgc cccacagcat gatcaagacg aagctgctga ccaaaactcg
    11641 agatacgcga tcgtgcaagg accaacgcag ggcatctgcc agcaatgcaa agactcgctg
    11701 tagggtcgaa gtacgctcgc gctccttcag catatcgact gccctcgaat ggcgcggccc
    11761 aaggcgcgta cccccatgcg ttcgctgaag ctgttctgga aggtacctcg ggggcacagc
    11821 ttggcgtgct gctcgagcca ggttacgaag gcggcgccgt agctcttgca aatgcgatcg
    11881 gcacccacgc tgaagttggc ctggccccaa tgggcgcgcg ctccgaattc attgaacagc
    11941 acccgctcgt agaagcgcag caagtcccag ccgccgggca cccgcgccag catcggcagc
    12001 tcgacgatgg cgctcgcccg accatgcgat agcgacagta gatggggcga ttcggcgacg
    12061 aagcgcaccg agaccggtac gttgtggtag cgcccgcgtg aggcttgctc tcgagccagc
    12121 tggaacagcc gctctagagc cgcgacatat tgaggcaggc ccgtcgcagg gtcaggctcg
    12181 ttcagtgcga acgcgagctc ggagccgtaa gcgggcacgt agttcgcgtc gcccaagtgt
    12241 agaacacgaa agctctcgtc cacgtacagc ccgtcgctca gactctggat ctctcgctcg
    12301 atgacccagg tcgaaaggcg cggccaaacg gtgagcgcca gcgcgtaaat ctcgtcgaac
    12361 tcgcggatgc tcgtgaacaa cgtcgcgacg aacgcgcgcg tgcggtctgg tcgtgtcttg
    12421 gcggcctcct tttccggcca gcggtaggtc accagacact ggcgcttgcc acgccgctcg
    12481 taagggctca ccagaaattc gaagtgccgg atcggcttgt ctcgcaagct gtgtagggtg
    12541 ggctgaaact cctgctgcac caactttctg cgaacctcct gccattccag gacttcgcga
    12601 tgctcaccga gatagtactc ggggcagacc tccagcacgt aggagtgcac gacccccatg
    12661 cagcccatcg ataccacgac gctgtggaac agagcatcat cctgatgtag ctcgatgtcg
    12721 gggcaaagcg tggcgtggga gcagggttcg ctgggcccgc cacacggttc gatccgcaac
    12781 gcgcgcccgc cctcggtgat gagatcgaga ctgcgtacca tgtcactgag cggtcccaat
    12841 gtccgacccg agccgtgcgt gcccgtcgaa atcgcgccgg ccaacgtttg tccgtcatag
    12901 ccacccatgt tgggaagggc gagaccgagc cgcttgaggg cttgattgag cgcacggatg
    12961 ctgatgccgc tggcggctcg cacgagctga ccgcgatcta cgccggagcg cagactcagc
    13021 gcgtcgagcg gcaacagtgt attccaatcc ttcaattcga gcagcacgtc gtccgcaatg
    13081 gcggcgtcgg agaaggagtg gccggagccc acggcgtgca cgcgtcgagg gggggttgcc
    13141 gcctcggcct ctcggataac gcgcgcgatc tgctcgccct cggtgatcga acgaatcgtc
    13201 gcgggccgtg cgaactggtt cttggtgacg ttctcgaagt gcccggcgtg ggtaacatcg
    13261 atcgagccgg gcaagggtct tggaagccag actcgaacca acgcttgggg gtaacgcgag
    13321 gcaacttgcc agagctgttg agcggtgatg agcaacgcga gcaccacgtg acccactaac
    13381 gccagcaagg tcgcgccaaa gcgaatcagt cttccgcaga cgccctcccg atcactcgag
    13441 gcagttggcc cgtgcatgtc tcaacctcgt atcctgaata catcgattaa atgggctcgg
    13501 ggcgcgggcg tgcggcccgc catcagctcg ccgcgggcca ccgaccgcgc gccaattacg
    13561 aaccccgtca agtcggttgc tccgctcgct agctcgcgca cccggaaacg aatgaaacag
    13621 acgtaatcgt ccagcaatgc aaaggtgccg ggcagcgagt aatgcgctcc caacgcatac
    13681 tgaatcagca tcgcccagcc gaacaacacc ggaatcacga tcgcgcctac gacgccgccg
    13741 agcagggccc ccaacgccgt gagtagcaca taagccagca ctccacgcgg tgtcggtggc
    13801 agactggact gtatgagact gtggagcggc tcgtgggggg cgagtgcgct cgcgagccga
    13861 gcggcaacga atagcaaggc tccaaccatc gcacagcccg agagcgtccc gagaacgaga
    13921 cttgccgctt ggcgtgcggg gtggagctgc tggcgattgt gggactgcag cagcgcgatg
    13981 ggacccagcc cgggccccag cgtaatcaat attacataga ccagcagatc gagcgtcact
    14041 cctggcgtgg atcgcccgcc cccgagccag accgcgacaa ggattgccaa actcgagaat
    14101 acgaatgtga ccgcgaccaa accaaagccg cgcgcaacct tggaccaccc gcgacgggcg
    14161 cgctgttcgc cgtccgaacg atcgaactgg aacagccggt gtccgacgcc gaaccacgca
    14221 ccaacggtga acagcaatag catggcggcc cagaacaccc aggccacgcc gtccggaagg
    14281 cgcggtgcac ccagagtgag ccagcccgcg cgaacgagcg agcgagagag ttctccctcg
    14341 tagctgcaca aggatgctcc gacgacggcg ccgaccacgg ccccgatgcc cgttacgccg
    14401 ctgcgcaaca gcatatgaac cgggttggca agacgtgaag tgatggtgcg gcgcgtcgcc
    14461 ttggggtgcg ggaaggcgtc gcaactctgc gttgcatgac ccgccaagtg gggcggtgga
    14521 tgcagcgtcg cgccgccgag ccccgagacg atcgagaagg ctccgcttcc taatccgtat
    14581 cgcgcgtagt gatgcgagtc ccccgataca tagaggcgcg cctgaggggg tctcggttcg
    14641 agctcgaggc cgagctcggc caacagttgg gccgcccacg actgagtctc gttgtaaacc
    14701 accagcggct tcgaagtgac gacgaccagg cgtttcggaa tctccgcgtg gcgtttgaag
    14761 aattgcttct gccggaagtc cacatcggtg ctgtcacgcg agtcgatccc ccaaatgcac
    14821 cagtcgaaag ggagatcaat cgcgacgtag cttgcttgtt ggaccgaagt gaaccccgcg
    14881 agctgaatca cgggagccgc gccggatggg gggattcgaa acaaccgatt gtaaccgtcc
    14941 aggctgtcgt accaatcatg gttggcggga attccgtaca gcggtcggct cgaacgtgtg
    15001 gatgtctggt tgccggggcg ccgggctgcg tgcgccaagt cgcagggagt ttgcaggcgc
    15061 gcgcgtaacg ttttttcgtc ggcgacggag taagccgcgt ccccgccgca gaaaagaaac
    15121 gccccggtcg gaagtcgcag cgcaaagtca tcgccccgat actgaatttc ccattcgcgg
    15181 acgcgccccg gcggggtgag cgtgggttca tccgcactca ggaggcgtgg atcatccacg
    15241 aataggtcac cgtgcagtag gtaataggca gaatacgtgg ctcggaacga gtcacccatg
    15301 tcagatatgt agtcgaacca gaactcgcgc gcgtcagccc gggccatcga cggctgagcg
    15361 ggttgcgtgg ttacggactc ggcgctcatc cagtctcttg gatccaggcg atccggaaac
    15421 caatcgaagg ccaacgtgcg tgtgagcccg cccaggaaat ttctgccgaa tccccaactc
    15481 acgggaccta ggatctccga aggtttgcgc ggcctccccc cgctttccgt cgcgatgaag
    15541 gcggggtcac cctcgtgtgg aatgtctcgc cactttgacg acaaggatga atcgtaacat
    15601 ttagtcagga tgggcgcgcg tggtcattgc ttcagcggat tggcaatttg aaccaacgct
    15661 cattgaccag gtggacactg agggtcttag ttccaagatg cccggagctg gagtgcgcgg
    15721 caaaggttcg aagctcgttg agcgaacgct gccgtgtggt ccgacgctac gcgttgggca
    15781 cgcacttgcc gtgggcgcgg tggtgttgct cgggaccagt cgcggtgacg cagctatcga
    15841 cgactttctg cgcggcgtgg ggcgcacact gggggcacct ttcggtggct tcgtcgaatc
    15901 gttggtcact ccctcgttgc aggcggggga gcaaacgggc aaggagctcg tggcggacgt
    15961 ggatcgacgc gtgggtgagc ggatccatca aatcggtgta accggcggag ctctattgag
    16021 tcgtgcggat agccttgccg caagtcggat cggacaagcc gatggcgcgg tggccgcgcg
    16081 tctgacgcag gctgacgcaa tgttgacggc ccgcattact caactcgacg tcggcgccaa
    16141 ccgcacgctc gaccgcgcct tgggccggct cgacggcagc gttcagtcgg cggtgcgcgg
    16201 gttgggcgca gaagcaagga gcgccgtccg ccagctcgat caagcgggca aggctcggct
    16261 cgagcaggcg gatgcaattt tggaatcacg cattgaccag ctcggcgccg tggtcaacca
    16321 atcgattgtg gacctggacc aaaacctcga gaatcgcctg gagcaggtcg acgacatcag
    16381 cgagcgccgc gtaggtaatc tggacgtggt cggcacgcgc caggtactcg gcgccgagag
    16441 tgcgctcacc cgactcgccg tgctcgctgc agttttgggg ttgatcgtga tcttcctaaa
    16501 gagccttggc gcggagattt gggagcgctg ggaagagaca gagcgggagc aggcgcgctg
    16561 ggcggggcgc gtgcgctcga tgctcagccc aagtctattc ttggggggcg cccgcgtgct
    16621 gctggcacac ttggcgattg cgggcgtatg cgtaggggta gtcttgctgg tcgccaagcg
    16681 cttgccgcgc gagaccgagc agcgctccca acagctgctc gcgacgcacc aggcgggctt
    16741 cgaggagagt atccagtcga ttgaccaccg gcgcgcactg tacttcgcct cgcaactcgc
    16801 gatcatcgat ccgctgcgat cgggtgagta tacgcggcga gccgagaaga ttcgtttggt
    16861 gcgcgacgtg ttggttcggc caggtttgtt tcaacggcct gacgccgtgc gcgaaacggc
    16921 gcggcgcatc gcggtattga gcgccgaact ccacgatacg gacggggatc tactcacgct
    16981 gcagtgctac gttcgttggc agctcgcgcg caatcgtgaa gacgaggctg cggccagcgc
    17041 cgactgccag cgtgcgctcg cgttgccgga ccactccccc ttccggttga ggccgctcgc
    17101 tcagcactac ttggcggccc tggcgacgtt ccctagttgg aggctcgcgg agctgcccag
    17161 ccctcccgtg ttcgagcccc cgagcgcgct gctcccgggc ttggagcata tattcactta
    17221 cgcggcgttg gtgcgtaagt tggacgagga cgcgacgcgc gcctatctac agatgttggc
    17281 ggcggagagc gatctccaca aggcgctcga acgtttgccc tcgaaaccca aggcgctccc
    17341 gccgcgcgcg ccgctttcac ccgagcagaa gacgttcgaa gatagcgtcg cccgtagatt
    17401 ggctcacgcg gagcaagtgg tggaagcctg gcgcgccttc gacgacgctt tgcagcacga
    17461 accgtggctg gccgggacga acaccgaact tgcggccttt ctgctcgatg atgcggtgtt
    17521 gactcgcgcc ttgatggtgg tcgcgcgtcc tcgggaaata cacttgggat tgcgtcttgc
    17581 ggagttgccg gaaagcttgc accgaatcga agccgccccc ctgcggatcg aatggatgcg
    17641 tcgttacttc ggcggcctgg gggagagcgt cgcccgcatc gtgtcttacg aggagtctcg
    17701 gcgtttcttg gaccacgagc gcgagctcgt tgaattcgag aaggcctacg tggcgttcca
    17761 tctgagcgaa agcccgaatg cgcagctgcg cttctcgcta gccctatcga gtgcccgagt
    17821 tggcttgtac gtgagcgagc acggccggcg gcggcccttt gctcaaactc tggggctcgt
    17881 ggaagcggca caaatctccg aagtgcagcg cgccgccgat gagcgacacg tgcgcctact
    17941 ctagtggatt gagccggtca cgtcgcggaa tgagatgggt agggccgctt cgctctcggc
    18001 ccgccgtctc cggcgctcct actgggatac tctccaacca ctggcgtttc agctgcgccg
    18061 agcggtcaag cgtcggccga gagcctctga gacaagctcc cagtcaggcg ctacctaccg
    18121 cccatgcgtg cggttgaggg ctcgggtgcg agggcgtagc agaaggctcg acgaagtatt
    18181 ttgcctcgcg gcgtacttag cggggcactc agtccgtatt cagatgctca gaggaccgcg
    18241 ggcgggaaac gccgctcgcg agccccgcca aactcgctct cttgccgact ctggagatcc
    18301 gaatgcattc taagttgtcg tgcccattct tctcgtccgt tgctcgaact tgggttgtgg
    18361 ggccgatcgg tgctgcggcg atgctgctca cggtcgcttg cggggggagc agcgataacg
    18421 agctcaacgc catgggcggc agcacgagtg gaacgagtgg aggcaccgcg aacgctaccg
    18481 gcggtgtgac gaactcgaac ggcgggacca cttcggtgag cggaggctct cagagcgggg
    18541 gagcgtccgc caccagcggt ggcgcgagca gcaccagcgg tggcgcgagc agcaccagcg
    18601 gcggtgcgac cacgaaggac ccattcagca ctccggtgac gtgcacgagc aacgtgaagt
    18661 ggacccgcgg tgagaacgac cgaatgcgcc ccggcgaagc ttgtattgcg tgccatgccc
    18721 aaactggcga cgcacccaaa ttcaccatcg caggtaccgt ataccctacg ggtcacgaac
    18781 ccaacgattg caacggcgtt accgccgcga ccgccacgac catcgtgatc acggacgcaa
    18841 atcaggtgga gcacaaaatt agcgccaatt ctgccggaaa tttcatgctt tctacgtcgg
    18901 tcgcgacgcc atacacggcc aaagtggtca cgtcagtcgg cgagcggaag atgttttccc
    18961 cgcaaacgtc gggagattgc aacagttgcc acacgcaaga tggctcgaat ggcgccccgg
    19021 ggcgcgtcct gatcccgtga gagtcgcaaa gcttccggcc ctctacgacg tgcagcggca
    19081 gcggcgctgg cgaatgcgag cggcgcgaac gcgcgtacgg cacggtgcat gttcggtttg
    19141 cgaccagttg ttggttttgc gacgcgggtt cctagcctaa actccatgct gctgcatcgg
    19201 cacgtcccgt gcagcgcgcc cgaccgatga ctcccatttc cgacaccgga tttgctcaac
    19261 tcgaatcaga agggcgactg ctgaatcccg tcttcaaagg cccaactcac gttccagggc
    19321 gctttgggtt tcggggtgag ctggcgctca agttcgcgtc caagcaagcc gacgaggcgc
    19381 gcccgcccga gctgatgtgc cagcaagttc tcgcgatcgc cgaacgacag cccacaatcc
    19441 cgttcttcgc gggttaccta ctgtcgtttg ccgaactcaa gccattggcc gaagcgttgg
    19501 gaaagaccat cagcgccgga ggcaagtact tcgttttctg caacaacatc gatctcggct
    19561 cccgctacca ggtgccttat gcgggcgcca tgtggtacgt gctcccaatc gacgaagcga
    19621 cggtctggaa cgaaatgctg gatctggtcc atctcgatcg aaacgatctc aaaaaactgg
    19681 atacggccgc gaaactcgac cggctcgctc aggccgccat tcagtacgac gtgaccttcg
    19741 atatgatgac ttacgccgag ggtttggagc gcatggggcc ggtccgcaat cccaatgaaa
    19801 atcgaccggt ctgagtgttt tcagcgccga agcgcggcgt ggaaaggctt gcgtcgagac
    19861 tacgattact cgaagtctcg acgcaatgtt ctacggacag ttcgccctct acggacagga
    19921 tcccaatcgg gccactctta gctcatcaga ccactcttcg gcgccggcgc acggccgcca
    19981 aagtgacggc cagcccaagc ccggctattg ctcccgtcga ggtggaccca ccgccgatgt
    20041 tgccgacgct gcacgtaaag cccggattgg acgagccgcc cgtccccgtc gaacctccgc
    20101 ggctggtggt tccgccgcgg ctcgtcgagc cgctcgaact cgaggagctg cccgccgcgc
    20161 caccgctagc ggccgtggtg ccacccgtgc tgttcgcgcc cgtgccggtc gattgcgtgg
    20221 tcgtggtacc ccctgcgctg gcgccggccg cagaagagcc ggcgctcgcg ccggtgccgc
    20281 tcgttcccgg attcgtgctc gtgcctcctc cggccccacc ggtaccgccg ttgggaacgg
    20341 tatcggcgcc tctcccaacg agtcgggagt gtacgcgccg cgcattcccc atgttcgtgt
    20401 agcgcacgta gctcaagagc agctgtcctt gtcgatcgct cgtcaacgtc acccctgcgc
    20461 aatctccctc gccaatccag gtcgagattt ctcccagggt gccatcctcc gccagattac
    20521 ggcccatcac gtgatggttc tcggtttccc acgcgagcca gtacgagctg ccatcccagg
    20581 ttgccgtagc gccgtagccg aggctctccg aggcaatct
//
LOCUS       DASTKU010000730         8341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_587769, whole genome shotgun sequence.
ACCESSION   DASTKU010000730 DASTKU010000000
VERSION     DASTKU010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8341
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587769"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFQ61_25585"
     CDS             <1..363
                     /locus_tag="VFQ61_25585"
                     /inference="COORDINATES: protein motif:HMM:NF021941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957906.1"
                     /translation="ELLTSEARFIEHLVGTQGKVVIGAAHGERPRGTVVSVTGDVEVL
                     VNLLGLVDAKKEHDRIERQLKKVTKDVTVMQKRLENPNFVNNAPPEVVSEAKAQLEQL
                     QRQKVRLEEARVLATELE"
     gene            complement(463..1263)
                     /locus_tag="VFQ61_25590"
     CDS             complement(463..1263)
                     /locus_tag="VFQ61_25590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957907.1"
                     /translation="MLRSTSRGAVSRIERCLPLIAASMLASVLSFLTSTAHAEDYYTR
                     RSFLAKDGGFEITGEPSRPEILRADISDDGPHAITLPPHFYFGVSRDVTLGITHRRGL
                     CLSACGDRHYNDVGFGLLVGLARGADYEIDLNTGLQFRSIDPAHFGWKGGVIGRVNFG
                     QIVGFVFDPTLYLAFTGRDTGNQHQLVLPFWFYFQASPKVAPFVGAMLVGPLDGFIDN
                     LSIPVEGGVMFSVSDRVDLGFDLRFTNLLGHHGGPAGRELGLLGRFQF"
     gene            1638..2651
                     /locus_tag="VFQ61_25595"
     CDS             1638..2651
                     /locus_tag="VFQ61_25595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9957908.1"
                     /translation="MAENPKGKSRKQPGFGLDREDPTGVPRRPQSEPASYAELDPDEA
                     TALVHVGSLGRIELGEEKNRHLLVRVKGAAIGQVFALPDEPCRIGRAPDSTLYVGDDG
                     VSRRHALIVKEGDGYVLIDTNSANGTFVGGERVGKHPLVDGDTIQLCSTAAFRYTVTD
                     VSQENLLQKLFEASVTDGLTGANNREHFDTMLRSELSYARRHKTELSLVLFDIDHFKR
                     VNDTFGHPAGDQVLIQISQAVATMIRSEDMFARYGGEEFALLLRGVALPGAVRLAERV
                     RATIESLLVACEKGSIRVTISAGCAALSELENPVGEALIQAADRRLYIAKNSGRNRVC
                     QTG"
     gene            2694..5534
                     /locus_tag="VFQ61_25600"
     CDS             2694..5534
                     /locus_tag="VFQ61_25600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9957909.1"
                     /translation="MTEPSKPPESGLPEHGVPERRDRADRSPRHSPAADWETAPTEQP
                     SELEFEEIRLPPGHPLRWSALLCAVGSFVLWTLWLARVGHSRLAVPVGTTFALLTSIC
                     VLRGLGCLDASRAPDAICSGRRGLRVLLMVGAGGLGFVGALRVAVAGVLPWPQWSSGV
                     LVTAAALALLAALARVPVTLGLAKPTRLYTQYGPWSLLALGVLLYVPWLGSFSLLDPW
                     ETHYGEVAREMLARDDWLSFWWAHERWFFSKPALDFWLQGSAFSLLGVQYQPGEMLAS
                     VQHGQLPAPEWAARIPIVVLSLVAVFLSYDAVARYAGRRAAWFSGAVLLTTPYWFLLS
                     RNGMTDMPYAAPVTSALALIALGLATPPHRVQKEIAFQFSRTRWTFTARSALLLLVLC
                     TVFPVLAYLASRNLSWVGLGHGAWPLRVQADHVLYGSGGQNCGLPGNDPCEQQAPQNP
                     LIQPALAALLWLLPLGLFLFANRGEYRVQRLYFIAAWYFTALATLGKGAPGLVLPVAI
                     GVAAVLVARRYERLVQLELVGLLLLVCCVTLPWYVQVYLRHGPEFTNRLLFYDMYQRA
                     FEHVHDTNSGEDVSFRYYIWQLGYGLFPWTSLSFVALACHRASLSAGSRKPAALVHYL
                     GLWALLTFCLFSASLTKFHHYILPAAPAVALITGLVAARAVGRSNERLADWAQAVVVG
                     FGSVAGAWLLSSGARQDAHHRALYLGVSCVCLMLTCGALFARKAAPSAKQAGYASAVF
                     GLAALLGAGWIALVTWDLSNPGSVPGDSRLLHLFSYNYARGFPSNLDFSAEFASFGWV
                     AATLCGLLLVPSLRAVLLRSSLLVACLFCVFVVNVYLPRIAPHFGQRNTLLEYYRARH
                     SADELLVAYQMNWKGENFYSGNRLAIFINTGQRLTSFLAERREAGRSVLFFTAEHSRL
                     SHLQLELGSVKSFRLLTTREQNDKFFLARVEL"
     gene            complement(5584..7131)
                     /gene="cobA"
                     /locus_tag="VFQ61_25605"
     CDS             complement(5584..7131)
                     /gene="cobA"
                     /locus_tag="VFQ61_25605"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HET9957910.1"
                     /translation="MAVGKVFLVGAGPGDPGLITVRGADVLSRADHVLTDALAHPALL
                     ELCEKAQIHWVGKRYGEDSAAQEAINRELVALASAGHLVVRLKGGDPLLFARGGEEAL
                     ALLDAGIPFEVIPGIASPVAASAYAGIPLTHRELSKSVTFITGSDRAGKEWSEEDWTR
                     LATSTDTLCILMGMRRIEEITQALLRGGRAPTTPAAVVQWGTRPEQRAVTGQLENIAQ
                     LSRERGLKNPAVILVGDVVQLRSRLAWFDVKPLFGKRLFLPRPAQQAKQSARAIRERG
                     AEPVVAPLIQIAPPPDAAPLERALETLSEYSWVVFTSSNGVDRFFAALHQKKRDARAL
                     GAARVAVIGPKTRDALLQFGIHADVVASEFVGEGLARDMIAASADAGGREPKKVLIAR
                     ALVARDALPNALRDAGFGVNVVPVYETRPVPDAERERVRELLQSGGIDVALFTSSSTV
                     SETLEVLGAQAQELLSRVTVASIGPITSKTLSEAGIRIDVTAREYTVEGLLDALESHF
                     AASSSAA"
     gene            7781..>8341
                     /locus_tag="VFQ61_25610"
     CDS             7781..>8341
                     /locus_tag="VFQ61_25610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957911.1"
                     /translation="MRHIQTSRFILIGLAALTALAANGCSSAEEAAAGCNGLDAKVQA
                     QASVKAFADAAFALRDRAVAIEGQFLAVCNKINAAVGADTSKTTAEGACGVLSGYIQA
                     QKLSVSVIVGGGCTIDAKAQAECEATCTPPSCDIEAECKGGEVVVACNGECSGSCDVQ
                     GPSVACQGSCEGECTSDVAVKCAGSCS"
BASE COUNT         1507 a         2658 c         2654 g         1522 t
ORIGIN      
        1 gaactcctga ccagtgaagc tcgcttcatc gaacacttgg tggggaccca aggtaaggtc
       61 gtgatcggcg cggcccatgg tgagcgcccg cgcggcacgg tcgtgagcgt gaccggagac
      121 gtggaggtgc tcgtcaatct gctcggtctc gtggacgcga agaaagagca cgatcgcatc
      181 gagcgccagc tcaagaaggt caccaaggac gtgaccgtga tgcagaagcg gctcgaaaat
      241 cccaacttcg tgaacaacgc tccacccgag gtggtgagcg aagccaaagc gcaactcgag
      301 caactacagc ggcagaaggt ccgcctcgag gaagcgcgcg tgctcgcgac cgaactcgag
      361 tgagcagccc cccaagtgag gggttgttgg agaacccgcc gcagcgaagg agcgagatag
      421 accgggctcc ttcgctgccg ctcgaaagcg atgcgagggc cgctagaact ggaaccgccc
      481 gagcaggcca agctcacgcc cggcgggccc gccatggtgg cccagcaagt tcgtgaagcg
      541 cagatcgaac ccgagatcga cgcgatcgct gacgctgaac atcacgccgc cctcgaccgg
      601 aatcgaaaga ttgtcgatga agccgtcgag cggccccacc agcattgccc cgacgaatgg
      661 cgccaccttg ggcgacgcct ggaagtagaa ccagaacggc aaaaccagct ggtgctggtt
      721 gccagtgtcg cgcccggtga aggcgaggta cagcgtggga tcgaaaacga agccgacgat
      781 ctgtccgaag ttgacgcgcc caatcacccc gcctttccag ccgaaatgag cgggatcgat
      841 gctccgaaac tgcagcccgg tattcagatc gatctcgtag tcagcacctc gggcaagccc
      901 caccaacagc ccaaaaccga cgtcgttgta gtggcggtca ccacaggcgg agaggcacag
      961 accacgacga tgtgtgatgc cgagcgtgac gtcgcgcgac acaccgaagt agaagtgagg
     1021 aggcaaagtg atggcgtgcg ggccatcatc cgagatgtcc gcgcgcagga tctccggccg
     1081 agacggctca cccgtaatct cgaagccgcc atccttggcg aggaagctgc gccgcgtgta
     1141 gtagtcttcc gcgtgggcgg tgctcgtgag gaaagagagc acactcgcca gcatcgaggc
     1201 cgcaatcagg ggcaagcagc gctcgatccg agaaacagct cctcgcgagg tggagcggag
     1261 cattttggag aatctaggca tctgtgggcg ggagcctaga gcatcgaagg ttttgaaacc
     1321 agcggtgaaa acgcctaggc gaggcgccgg aacgagcccg cagcgcgccg ggaaagccaa
     1381 cgcggcgccg agaagccccg cagggcggtg ggcagcgaga gaagctcgcg agcggatcaa
     1441 tctgcagtcg agccgcatgt tcacgcgcgc taacgcgcgt ctgctcggag tgcgcccctc
     1501 accacagacc gtaggcgcgg gtccgagtgg gcgagattcc gtatgtagat cagcgcgacg
     1561 cgcgctgacg caaaggcaag ttcgtcggga atcgtagctc ttgcagagcc tcgggatcca
     1621 ggtacgttga gaagtacgtg gccgagaacc ccaaagggaa atcccgcaag cagcctgggt
     1681 tcggtttgga tcgggaggac cccaccggtg taccgcgtcg gccgcaaagc gagccagcaa
     1741 gctacgccga gctggatccg gacgaagcga cggcgctcgt ccatgtgggg agcttgggtc
     1801 gcatcgagct tggcgaagag aagaaccgcc acctgctcgt gcgcgtaaaa ggtgccgcga
     1861 ttggccaggt ttttgcgttg ccagacgaac cctgccggat cgggcgcgcg ccagattcga
     1921 ccttgtacgt cggcgacgat ggtgtgagcc gccgccacgc tttgatcgtg aaggagggcg
     1981 atggctacgt gctgatcgac acgaacagcg ccaacggcac cttcgtgggt ggggagcgcg
     2041 tcggcaaaca tcccttggtc gacggagaca ccattcagct ctgctccacg gcggccttcc
     2101 gctataccgt caccgacgtc agccaggaga atctgctcca gaagctgttc gaagccagcg
     2161 taaccgatgg cctcacgggt gcgaacaatc gagaacactt cgataccatg ttgcgctccg
     2221 agctgagcta cgcgcggcga cacaagaccg agctatcgct ggtgttgttc gacatcgatc
     2281 acttcaagcg cgtcaacgac acgttcggcc atcctgcggg tgaccaggtg ctgatccaaa
     2341 tctcgcaggc cgtcgcaacc atgatacgca gcgaggacat gttcgcgcgc tatggtggtg
     2401 aagagtttgc tcttctgctg cgtggggtcg cgcttcccgg agccgtgcgc ctggccgagc
     2461 gcgtccgcgc aacgatcgag agcttgctcg tggcgtgcga aaaaggctcg attcgggtca
     2521 cgatcagcgc gggctgcgcg gcgttgagcg agctcgaaaa ccccgtcggt gaggccctga
     2581 tccaggctgc cgaccgtcgg ctctacatag ccaagaactc aggccgaaac cgagtctgcc
     2641 agaccggttg atcgttgctt cgagccgggt tgtgggcgaa aaccgctacg cccgtgaccg
     2701 agccatccaa gccccccgaa agcggacttc cggaacacgg agttccggag cgtcgcgatc
     2761 gagcggacag gtctcctcga cacagccccg cagcggattg ggagacggcg ccgacagagc
     2821 agccatccga gctcgagttc gaggagatcc gactgccccc gggccatccg ctgaggtggt
     2881 ccgcgttgct gtgcgccgtg ggcagcttcg tgctgtggac gttgtggttg gctcgggtgg
     2941 gccattcgcg cctggccgtg ccagtcggca cgacgttcgc gctgctcacc agcatatgcg
     3001 tgttgcgcgg cctcggatgt ttggatgcga gtcgggctcc cgacgcgatc tgctcgggac
     3061 ggcgcggcct gcgcgtgttg ctgatggtcg gtgccggtgg actggggttc gttggagcgc
     3121 tccgtgtggc cgtcgcgggt gtgctgccat ggccgcaatg gtcgagcggt gtactggtca
     3181 cggcggccgc gctcgcgctg ctcgccgctc tcgcccgagt cccggtcacg ctcgggctgg
     3241 caaagcctac tcgtttgtac acgcagtatg gtccctggag tctgctcgca ctcggcgtgc
     3301 tgctctatgt gccgtggctc ggcagcttct cgctgctcga tccgtgggag acccactacg
     3361 gtgaagtggc gcgcgagatg ctggcgcgcg acgactggtt gagcttctgg tgggcacacg
     3421 agcgctggtt cttctccaaa cctgcgctcg acttctggct gcagggcagc gccttctcct
     3481 tgctcggagt ccagtatcag cctggagaga tgctcgcgag cgtgcagcac gggcagctgc
     3541 cggcgcccga atgggccgcg cgtattccga tcgtcgtgct gtccctggtg gccgtgttcc
     3601 ttagctatga tgccgtggcg cgctacgcgg gccggcgagc ggcgtggttt tccggcgcgg
     3661 tgctgctcac cacgccttac tggttcctgt tgtctcgcaa cggcatgacg gacatgcctt
     3721 acgcagcgcc ggtcacctct gcgttggctt tgatcgctct cgggctcgcg acgccgcccc
     3781 atcgcgtgca gaaagagatt gcgttccaat tcagccgcac gcgttggacc ttcaccgctc
     3841 gtagcgcgct gctgttactc gttctctgca cggtatttcc cgtgctcgct taccttgcct
     3901 cccgcaacct cagctgggtt ggattggggc acggcgcgtg gccgctgcgc gtccaagcgg
     3961 accacgtgct gtacggctct ggtggtcaaa actgcgggct ccccggcaat gatccttgcg
     4021 agcagcaggc gccacagaat ccgttgattc agcccgcgct ggccgccctg ctttggctcc
     4081 tgcctctcgg cttgttcttg ttcgccaacc gcggggagta tcgggtgcag cggctgtact
     4141 tcatcgccgc gtggtacttc accgcgctcg ccaccttggg caagggtgcg cctgggctgg
     4201 tgttaccggt cgcgattggg gtggccgcag tgttggtagc tcgccgctac gagcgtttgg
     4261 ttcagctgga gctcgtgggc ttactgctcc tggtttgctg cgtgacgctg ccctggtacg
     4321 tgcaggtcta tctccgacac ggtcccgagt tcacgaaccg gctgctattt tacgacatgt
     4381 atcagcgtgc tttcgagcac gtgcacgata ccaacagcgg ggaagacgtc agctttcgtt
     4441 actacatctg gcagctggga tacgggctct tcccttggac ttcgctgagc ttcgtcgcgc
     4501 tggcctgtca tcgagcgagc ttgagcgccg gctcgcgaaa gccagcggca ttggtgcact
     4561 atctgggcct gtgggcgctc ctgacgttct gtctattcag cgcttccttg accaagttcc
     4621 accactacat tctgcccgcc gcgcccgccg tcgcgttgat taccggcttg gttgcggctc
     4681 gcgcggtggg gcgcagcaac gagcgtctgg cggactgggc gcaagcggtc gtggttggct
     4741 tcggtagcgt cgccggcgct tggctgctca gctctggcgc tcggcaagac gcgcatcatc
     4801 gggcgctata tttgggcgtc agttgtgtgt gtttgatgct gacttgcggc gcgctgttcg
     4861 cgcgcaaggc agcgccgagc gcgaagcaag ccggctacgc ttccgcggtt ttcggcctgg
     4921 ccgcgttgct cggggcgggc tggatcgcgc tcgtcacctg ggatctgtcg aacccgggca
     4981 gcgtgccggg cgacagccgc ctgttgcacc tgttcagcta caactatgca cgcggcttcc
     5041 cctccaacct ggatttctcc gccgaatttg cgagtttcgg ctgggtcgcg gccacactgt
     5101 gcgggctctt gctggtgccg agcttgcgcg cggtactgct acgcagttcg ctgctcgttg
     5161 cctgtctgtt ttgcgtgttc gtcgtgaacg tctacctgcc gcgcattgcg ccacacttcg
     5221 gccaacgcaa cacgcttctc gaatactatc gcgctcgcca cagcgccgat gagctgctcg
     5281 tcgcctatca gatgaactgg aagggggaaa acttctattc gggcaaccgc ttggcaatct
     5341 tcatcaacac cggccagcgc ctgacgagct ttctcgcgga gcgacgcgag gccggacgaa
     5401 gcgtgctgtt cttcacggca gagcacagcc gactgtccca cctgcagctc gaactcgggt
     5461 cggtgaagag ctttcggctc ctcaccacgc gtgaacagaa cgacaagttc tttttggcgc
     5521 gcgtggaact ctgagtccac gcatcaccgc aaatatcacc tcaccgcggc atccccgcgc
     5581 ggctcaggcc gcggacgagg acgcagcgaa gtggctttcg agcgcgtcga gcaagccctc
     5641 cacggtgtac tcgcgggccg tcacgtcgat gcgaatcccg gcttcggaga gggtcttgct
     5701 ggtgatgggc ccgatgctcg ccaccgtcac cctcgagagt agctcctgcg cctgcgcccc
     5761 cagcacctcg agcgtctcgc tcacggtcga actggaagta aagagggcca cgtctatccc
     5821 gccactctgc agaagctccc tgacccgctc gcgctcagca tccggcaccg ggcgcgtctc
     5881 gtaaacgggc accacattca ccccaaaacc cgcgtcgcgg agcgcattcg gcagcgcatc
     5941 gcgcgcgacc aaggcgcgcg cgatcagcac cttcttgggt tcccgacctc ccgcgtccgc
     6001 cgaggccgcg atcatatcgc gcgccaaacc ctcgcccacg aactcactcg cgaccacgtc
     6061 cgcgtggatc ccgaactgca gcagcgcgtc ccgagttttc ggaccaatca ccgccacccg
     6121 cgcggcgccg agagcgcgcg catcacgctt cttctgatgg agcgccgcga agaagcgatc
     6181 gacgccattg ctgctcgtaa acaccaccca ggaatattcg gacaacgtct ccaaagcgcg
     6241 ctcgagcggg gccgcatcgg gcggcggcgc gatttgaatc aaaggtgcca ctacgggttc
     6301 ggcgccgcgc tctcggatgg cccgcgcgct ctgttttgct tgctgcgccg gtcgcggcag
     6361 aaatagccgc ttgccgaaca gcggcttcac gtcgaaccac gccaggcgag agcgcagctg
     6421 aaccacgtca ccgaccagaa ttacggcggg gttcttcagg cctcgctcac ggctgagctg
     6481 ggcaatgttt tcgagctgac cggtcactgc gcgttgctcg ggacgggttc cccattgcac
     6541 cacggccgcg ggcgtcgtgg gcgctcggcc gcctcgcagc aacgcctggg tgatctcctc
     6601 gatgcgccgc atgcccatca agatgcagag cgtatcggtc gaggtcgcca ggcgagtcca
     6661 gtcttcttcc gaccactctt tgcccgcacg atcactaccg gtaatgaagg taacgctctt
     6721 agagagctca cgatgtgtga gcgggatccc cgcatacgcg ctagccgcga ccggcgacgc
     6781 gatgcccggg attacctcga acgggattcc ggcatcgagc agggctaagg cctcctcacc
     6841 gccgcgcgcg aacaacagcg ggtcaccgcc tttgagacgt acgaccagat ggccagcgga
     6901 ggccaacgcg acgagctcgc gattgatggc ttcctgggcc gcgctgtcct cgccgtaacg
     6961 cttcccgacc cagtggattt gcgccttttc gcacagctcg agcaacgccg ggtgcgcgag
     7021 cgcatccgtg agcacgtgat cggcccgcga gagtacgtcc gctccacgca ccgtaatcag
     7081 ccctggatcg cccggacctg cgccgaccaa gaacacctta ccgacagcca ttcggacgcg
     7141 gttctacgac gaaacgacgg tcgtgcgaat cggatgcagg ttcctgcgga ttttcggcag
     7201 actgcaacgt cggctgaatc cgagggccgt cgaaatcgag cggcgcgcat ccacgtcagg
     7261 aaaacgacgc agactgtcag cagcaatcga ccctcgttcg aacaggcgcg ccactgcacg
     7321 cgtgcccctc ggttcacgcg actatgactc gaagatccgg gtgcttgggc tggccgaaag
     7381 cacgcgcttc gacgatctca ggcaaatcat cgacgaggag ggacctcgcg cacgagcgcg
     7441 agcgaacggc ccaaggactg caaagcgcgc gcagacagcc ggctctcagc acttccgaaa
     7501 ccacgtgggc aacatcgcca aaacgcacga ctccatggcc gctccgcttc atcacgacgg
     7561 ttccgcttcc tcatacggtt caaatgcata agtgtgaggg tctgggtgtg aggagcattc
     7621 ttcacttgtc tccacaagtt gagtaccgtg cggggctcgg tgacgctccc gactcatcgg
     7681 ccttaaggcc aatctcgagt tcgcaggacc ttacttagaa ccgtcgttgc ttcggttccc
     7741 ctgcgcttcc gtccaaggca atcaaaggag aagcttcctc atgcgacata tccaaacttc
     7801 acggttcatt ctgatcggct tggcagcact caccgccctc gccgcaaacg gctgcagctc
     7861 cgcggaagaa gccgcagcgg gctgcaatgg tctcgacgca aaggtgcagg cccaagcctc
     7921 cgtgaaggca tttgccgacg cggctttcgc actccgcgat cgcgccgtcg ccatcgaagg
     7981 ccagttcttg gcggtttgca acaagatcaa cgccgccgtg ggcgcggata ccagcaagac
     8041 tacggccgag ggtgcctgcg gtgtattgag cggctacatc caagcccaga agctatccgt
     8101 gagcgtaatc gtgggcggtg gctgcaccat cgacgccaaa gcgcaggctg agtgcgaagc
     8161 gacctgtacg cctccaagct gcgatatcga ggccgaatgc aagggtggcg aggtcgttgt
     8221 tgcttgcaac ggtgagtgca gtggaagctg tgacgttcaa ggcccttccg tggcatgcca
     8281 aggatcttgc gaaggcgaat gcacctcgga cgtcgcggtg aagtgcgcag gctcctgctc
     8341 c
//
LOCUS       DASTKU010000731        19500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1259105, whole genome shotgun sequence.
ACCESSION   DASTKU010000731 DASTKU010000000
VERSION     DASTKU010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 19500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19500
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259105"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFQ61_25615"
     CDS             <1..271
                     /locus_tag="VFQ61_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HET9957912.1"
                     /translation="ANAGEEGSIVVQKIKEGSGNFGFNAATTKYGDLVADGVIDPVKV
                     VRSALQNAASVAGLMLTTEALVAEKPKEEKAPAPGGHAHGPGGDF"
     gene            complement(360..1328)
                     /locus_tag="VFQ61_25620"
     CDS             complement(360..1328)
                     /locus_tag="VFQ61_25620"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9957913.1"
                     /translation="MGALVLLVDDSPTIRSQIRLALQQVPEFERFIEAGDGLQAFKMM
                     VDARPDLVVCDLVMPVFDGLKFLALRHTRADLAHIPVIMLTAEGDSNRKVEVLDRGAS
                     DYVTKPFNEKELLARVRVHYRLKVLQDELRTANARLESLAVTDGLTGLFNRRYFDTLL
                     ITEMQRTLRYKTPIAVALLDIDHFKHVNDTYGHPMGDEVLRNLSRLVASSVRTTDSVA
                     RYGGEEIAVVLTQTDEHGALEVAERLRVQISELTHTLGEHSIQKTASFGVACFDGNGD
                     AMHPEDLVKRADQALYRAKHGGRNRVVLWTPDLQQEAEPSSVVAHL"
     gene            complement(1442..2416)
                     /locus_tag="VFQ61_25625"
     CDS             complement(1442..2416)
                     /locus_tag="VFQ61_25625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957914.1"
                     /translation="MRWLSAFVIGLCTTWSGFAVAQNPEPAATPSASAAQAEALFNEG
                     RKLVSAGKFAEACPKFEQSERLDPALGTLLNLADCFERVGRVGSAYRGFLDAAARAES
                     EGYSDAVRGARERAEALAPQVPKLVVIVPTQSNLRVTQDGQTLEPASWGQPIPVDPGA
                     HHIAASAPGKQPWETNVVMEKGATKTVQVPELGEGASSAAGVALEPSDAVSPGSFQWT
                     GARIGAVAAGGVGVVGIGLGTFFGLKSMSKRDDAEKHCTGSTCTDDEGVELRSDARAA
                     GNASTVAFAAGAVALAAGAVLWFTARDAEAPTAGLRVGPTSVALTGSF"
     gene            complement(2413..3957)
                     /locus_tag="VFQ61_25630"
     CDS             complement(2413..3957)
                     /locus_tag="VFQ61_25630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957915.1"
                     /translation="MEGFVGPGQTLAGGKYRVERILGQGGMGVVVSALHVPLGQHVAL
                     KFLQPAMAANPGATQRFLREARAAAQIQSEHVARVMDVGTLDFGAPYMVMELLRGSDL
                     ARWLREKGPLTVEQAVSYVLQACEAIAEAHSLGIVHRDVKPANLFLTQRADGSPLVKV
                     LDFGISKAAVCDTDEFQVSVTSTNAVMGSPRYMAPEQLKNAKDVDARADIWSLGIVLY
                     ELIAGVPPYPGETPWDLMSAIISDEIPALRTVRPDAPALVDRVISRCLEKDRERRFSN
                     LAELVVALKDAAPPEARVSIDRTVRIIRGNQQGLTSPGEAARAEGASPATFTASAWDS
                     HPARHLPLRRNIGIGVASVAGVALLGVGAFALWGHSETPDHPSTVHALSSGSISSEVG
                     SLRARPREAASETGSGLGVPRVEPEPTSAETAASSQASEVKTSAPSGAAGATAGAAAS
                     GEPKGTQASQASAKAQPRPAKPAVASPAPTVAKPVSTAGTPAVKSRPSLTDLMQSRKP
                     GHGKSP"
     gene            complement(4169..5725)
                     /locus_tag="VFQ61_25635"
     CDS             complement(4169..5725)
                     /locus_tag="VFQ61_25635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957916.1"
                     /translation="MKRTTALGFLGVCLAPWVVACGPQSSENVSAQSLAVTAQANAET
                     TLRGLSNSAGFVAESASLAQALSAVSGSTVCSTSAAVPCPIGSTTCPQPVTTCETEKV
                     EVSDLQDSHDELNDSINSLVRELKEKIFIPANLESETSTAATYLLGPKVLCEETESVA
                     VPGGTDAAPSQTTGLDPDCVEQAQKLAIRLRLSPLGAQGVSVALLLTSAQRNPLTLEL
                     EPARAALNLDLGELQGALDALGEDTGSVNRMSGKLQAQIVKNAERNYTLSLSVLQALA
                     LDLENDFGEKVAVSLAPSSPTLELGLDGVERKLTARMDYGAFGVRGPLNAFRDRFDDP
                     APTATGSDVAAPAYTGNIDLSVAGLEGTVTLDGAQDRLKLVGLGLGDAPSTLKFEGTT
                     VLEANLNPNSGRHFDVDVQKVGENASLTFSSFDLSLLMNFSPLAVQIADLPKFMLNDS
                     MRFFLQGDKPALQVEKEQVRVLSGTLNMTSQAQPGASIVVAPGNCLLDSGIDDAPHEL
                     LGAFAAGACH"
     gene            complement(6101..7834)
                     /locus_tag="VFQ61_25640"
     CDS             complement(6101..7834)
                     /locus_tag="VFQ61_25640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957917.1"
                     /translation="MNRLFACVAVLELVGCAKSTQISTPFSTDWLSDSGQSIAAVEAS
                     LRNAPRPRNQAVVLAITDRALQGVSLASGQRWSYAAAPDSAPSVAGDLVLFTSKGVLH
                     ALDAGSGKSRFQVSVEDMVLKGAGDDGSTTVVTLGTFDGRRSLFLALDHAGHEKTRLP
                     TSIALGRPAVLGGVAFIPWSGQYVSAIDTSNSEELGRLLTREVVSHAFTAGGELFFGE
                     RAVLHFDQRIRDASTRQSGRVALPERVLPGQPRWLGPGSEVSIVDPSARAKIRLYASP
                     RYDGQQVSFASNAFLATYFRAVMSFDAKTAELRAVQALPGAVLGGSAARSGFVLCGAD
                     GKVTYFGTQGGVLDGPDFKTPVRGCVVEANEFELPKTNSAGTLAAQLDRTLLSLEPEM
                     AAAQAFLMTELGRLPDPSVSKTLIDLASSARLAPDLRARARQLLAQRRAGAEFMLAAL
                     ERRYDFLASDALPPPVGPLADALAAMNEKRAAPLLARHLSDPATELADLEHAAKALET
                     LATAAELPSLKTFFALYRATADEAPLVAAVVSVARALVRVGGPEERALVERAQHDPLT
                     QPDVARDLAGV"
     gene            complement(7831..8562)
                     /locus_tag="VFQ61_25645"
     CDS             complement(7831..8562)
                     /locus_tag="VFQ61_25645"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9957918.1"
                     /translation="MERWRKPLESSNESELLAALEELSGLGESAGAAAPMVNSLLARG
                     ANTKVLLSALNTAALLGQASSSDFVAPYVSHRNTEIRRAAARTLARTKGATAVTALRQ
                     ALTGQDPVTRGLAAAGLGQLGAKEAVNELFAVLVRDTPEAAVSIASLCSPEQCDRLMD
                     FVGKLKFQVLEASFVPLLLRPQAELPDANKIRYIDRLRRLQTKGAASVLETVSASLPK
                     EESAAVRAALTAALRARPVVGDPSP"
     gene            8570..9568
                     /locus_tag="VFQ61_25650"
     CDS             8570..9568
                     /locus_tag="VFQ61_25650"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9957919.1"
                     /translation="MAFAPAAALPVEGLAAVRPGLAGALGLGAAGAEFTAGGFDAGGF
                     GAAAASPDAVDTRASQGKQRSILSPLARDRSRWRGRRRHPDVLWLAYARKPLSHTEFT
                     MPSSPSLPELPLIRLELVEDQSPPHQTGFLRLVRRRFRAIYPDGSRSAEFTYDEVDRP
                     AIDAVVIVAYYLDAHGERRVFLRSSLRPPIVLRDRTRSPRPGADPVSSIWELAAGLVE
                     QDEQTESGLKTSAIRELEEELGFRASPDQVFELGPSTFPAGGVIAERHFYFGVRVDPK
                     SRQDPGLDGSALEHGGVVTDVTLTEALAMCRDGRIVDAKTELGLRRLAETPVERPT"
     gene            9565..10506
                     /locus_tag="VFQ61_25655"
     CDS             9565..10506
                     /locus_tag="VFQ61_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET9957920.1"
                     /translation="MTSAKASRTRTPTATKAAEPRIIVVTKRTSYRRLILEERDPRAR
                     NLISRKDPSVSSWVRAHREHERTLETVERVLGKSGLPVLWVRRAHALFDTSGASLVIA
                     IGGDGTLLAASHNVDTVPLLGVNSAPNHSVGFFCAARRENFAELFEQALERRLRHVTL
                     TRMKVVVNGRLRSSRVLNEALYAHSSPAATSRYLVELDGLREDQRSSGFWIGPASGST
                     AAQRSAGGRVMPLSSKQLQLVVREPYAPFDQKYRLLKKQFSPDQTLIVHSKMDDAMLW
                     LDGPNRQIPIRLGDIVEFGVSDMPLTVLGLSSKRTIL"
     gene            10520..11188
                     /locus_tag="VFQ61_25660"
     CDS             10520..11188
                     /locus_tag="VFQ61_25660"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET9957921.1"
                     /translation="MTGLLRTRIKACCIASITEARIAIQAGASAIGLMPRMPDGPGVL
                     SEAEVREIAEATPPGVTSVLLTTQLDVDQIVTQQRGSMTHTLQLCAPLTPDARLRLRR
                     ELPGVRLLQVVHIEGEESVAQALDVQRTADALLLDTQIKRDRFVQLGGTGLTHDWAIS
                     ERIVKQANIPVFLAGGLRATNVAEAIRRVRPFGVDVCTGIRAPHGLDPEKLEAFVTAV
                     AEAS"
     gene            11279..11821
                     /locus_tag="VFQ61_25665"
     CDS             11279..11821
                     /locus_tag="VFQ61_25665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957922.1"
                     /translation="MSAEFRSGRCLAGWIAVLTVVLGACARNAPAGGSAESAPSAPSV
                     TPAAGSMPSISARTSSPASASAPNSDPTRDPEHTTPEPSSDCPTAASLGATALLELEA
                     TTSAEQAAIQAVRRELTRVLENPGSYFGTVQTASDLILVELWHKSGLSPEAGCHAPGK
                     RRTMTFDPRAGKIVATKIWE"
     gene            complement(11843..13009)
                     /locus_tag="VFQ61_25670"
     CDS             complement(11843..13009)
                     /locus_tag="VFQ61_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HET9957923.1"
                     /translation="MRIHSKLPQVGTTIFTVMSRLAEQYGALNLGQGFPDFEPPAALL
                     EALARCAAQGRNQYAPMAGIPALREQIASKLRRDYGRALDPESEITITSGATEAIFDV
                     VAALVGADDEVILLDPSYDSYGPAVALQGARARHVPLDAEHFSIDFERLRAALTPRTR
                     LLVINFPHNPSGAVLGAEDLDRLAGLLRDTSCWLLSDEVYEHIVFDGGAHQSVLRHPE
                     LAERSLQVGSFGKAYHATGWKVGWIAAPRALTEEVRKVHQFVTFSTNTPAQHALAEVL
                     VSAPEHLHELSGFYQRKRDLFRSLLSSSRLQFPSTRGTYFQLADYSALSRLPDQAFAE
                     RLVREAGVAAIPISGLYQAGSSRQIVRFCFAKGNDTLERACERLLRFEEKLLNE"
     gene            13094..14335
                     /locus_tag="VFQ61_25675"
     CDS             13094..14335
                     /locus_tag="VFQ61_25675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HET9957924.1"
                     /translation="MATASVLSIGTEITRGELVNTNASYLAEGLTARGFEVTEICTVD
                     DDAPRIEACLARLGGSCDVIVSTGGLGPTTDDITTECVARLLGVPLRRDPESFAILEQ
                     PLRDFGRSVSASNAKQTDFPVGASVLPNPNGSAPGFCVRIARALAFFMPGVPREMKPM
                     FESSVLPAVAPLVDVASHQIHLQTFGLPESEANDRLAGVEAQFGVLIGYRVSMPVLEI
                     KVLARDASASVAETRARTAADEVRRRLGDVVYAEGHATLPEVLGAELKSRGLRLACAE
                     SCTGGLVGELLTDRPSASEFFAGSAVVYENRQKTRLLGVDAALIEQHGAVSEPVARAM
                     AEGALRVFEVDVALAITGIAGPGGGTETKPVGLVHYAVATPRGTTARSFVFVRDRYMV
                     RRRAAFAALDLAHRVIRQGHP"
     gene            complement(14401..15336)
                     /locus_tag="VFQ61_25680"
     CDS             complement(14401..15336)
                     /locus_tag="VFQ61_25680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957925.1"
                     /translation="MKRDAFEEEIAVNPGASIVVSADFLRLRLLPSGRPDRFYIGGDH
                     PVLRQRLRIDRNPNAVRLALGSANHELPWPFREFLSSHGELRLHIPDGALLDVTASAG
                     RISAEHLSGGLNIRSDAGRIDIHSFRGNLRIAASAGRIEGTELEGTIDVSSNAGAVRL
                     EVVSLAPGEHRVQTNLGTARVDLPRGLPVAIEARTAMGSASIAFPSTPTADAVLKLSA
                     DLGSVSVRESARIWDPRLPPRVTAGLASRPVRVATAGFAEVSRPGQSLEAADREQALA
                     LRVSDEDWQRVQRRVTRGTLSPDVARELLRVLGHR"
     gene            15472..17130
                     /locus_tag="VFQ61_25685"
     CDS             15472..17130
                     /locus_tag="VFQ61_25685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9957926.1"
                     /translation="MGSDENPKDRPPDSLADGAETPRRIGNYRVIRQLGYGAFADVYL
                     AQHEQTHLQVALKVLRAQLDPRLAEQIRLRFLAEERIARTIDDARIVRIYETSTGEEA
                     GPCFIAMEYVDGKPFCEAFRGFEPLTDEERQLKLSQLGMMVAQAVARAHERGIVHRDL
                     KPDNVLVTRGPRHGIKILDFGIAKAPISLLASLPKPAGSLTSQLVTELGTVMGSRPYM
                     APEQNGAAHAVTGKADVYALGVMLAITAAGLDADALETSGASWVLQEDVDRVIDTRPL
                     SNEFSQLLRRMLELEPEARPEMAEVALELQRWSRPERAFADAVYAFRKHAKMPTKGAL
                     LRVLRDVEHEAHFTEDELEFLRLVPARSLASTGAGLPWALVGLCAASAAALGVTYVRQ
                     REQAQSDQAKLRVQNADVLKLYADLKQSVGQPGETGTADARELRLASAAAPKSDAKAQ
                     ERSAPVCRNNAEQLAECVERRRGAERQAAECSGRSENLAQNLETQRRELDAHVQAEAQ
                     CRRELDTRIAQLAECTSRVRLCSESLRATNSVRTVPADPDGAVE"
     gene            17292..18020
                     /locus_tag="VFQ61_25690"
     CDS             17292..18020
                     /locus_tag="VFQ61_25690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9957927.1"
                     /translation="MPRREGAAAIANATPPSSPRGSHENPDPERPPLSGPETGSTPPE
                     LPRAAMVLQVGDSFADALSKPLKARLLAEGLRTAVEVKTASYIGTWAYGADLPRFVAR
                     YKPDLILVTLGANELSIPEPEARQSAIRRLVGSISSHPCVWITPPRWKKDTGVLEVIR
                     RNAAPCRFLDSDQLVGELPRGPDKIHPTKQGSERWAELVLGWLRHERDPSGATPFALK
                     PEPLQPGAPRVETSQSATHDPSRQ"
     gene            18216..18728
                     /locus_tag="VFQ61_25695"
     CDS             18216..18728
                     /locus_tag="VFQ61_25695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957928.1"
                     /translation="MNRVASPLPLSHPPDELRGLKLSFRPNLELIASTRRLVEDLLDV
                     ELTDPDARSRIVVAIHELIENTYKYSSDGAATLDVSFARSNAGGTRVCILASNRTLPG
                     RAKELQERISSLGRLSDPMSAYVRWMTESAQREGSGLGLLRIRAECEMQLSAALDGDR
                     ITIEATALVS"
     gene            complement(18795..>19500)
                     /locus_tag="VFQ61_25700"
     CDS             complement(18795..>19500)
                     /locus_tag="VFQ61_25700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957929.1"
                     /translation="IASRSLWLERSTVAVENGYFSVRLGEETPFPAGLFESEGNLYLG
                     IRIADDEELKPRQKITSVPFALRSAYADEAGAAVGELAQQIVPSGAVMPFDLEVCPAG
                     WAAFEPARGRTIVGTNPVDPDSANSNKLSRRVRGERFGEESHTLTVEELPAHHHTGAT
                     DRGARMNYRVYYSAGESYFTNRSIGLGSGAYTERNDTAWPMSDHTHTFTTNDTGEGQA
                     INLTPPSVVLLYCRKL"
BASE COUNT         3551 a         6315 c         6232 g         3402 t
ORIGIN      
        1 ggcgaacgcg ggcgaagaag gctcgatcgt cgttcagaag atcaaggaag gcagcggcaa
       61 cttcggcttc aacgccgcca ccaccaagta cggcgacctc gttgccgacg gcgtcatcga
      121 cccggtcaag gtcgtgcgct ccgcgctgca gaacgccgcc agcgtcgccg gcctcatgct
      181 gacgaccgag gcgctcgtcg ccgagaagcc caaggaagag aaagctccgg caccgggcgg
      241 tcacgcgcac ggtccgggcg gcgacttctg atcggtcccc ccacctcgac actgaggtca
      301 acagcgcgcg gctcggtttc gactgggccg cgcgtcgatc ttttgtacgc cgaagcggct
      361 cagagatgag caaccacgga gctgggctcc gcttcctgct gcaggtcggg cgtccataag
      421 actacccgat tgcgtccccc gtgcttggct cgatagagcg cttgatccgc gcgctttacg
      481 agatcctcgg ggtgcatcgc gtccccgttg ccgtcgaaac aggccactcc gaagctggcc
      541 gtcttttgga tcgagtgctc acccaaggtg tgcgtcagct ccgagatttg cacgcggagg
      601 cgttccgcca cctctaacgc accgtgttcg tccgtttggg tcaagaccac tgcgatctct
      661 tcgcctccgt agcgcgccac cgaatccgtc gtgcgcacgc tcgaagccac taagcgactc
      721 agattgcgga ggacctcgtc acccatgggg tggccgtagg tgtcgttgac gtgcttgaag
      781 tggtcgatat ccagcaacgc gacggcgatt ggggtcttgt agcgcagagt gcgctgcatc
      841 tccgtgatca gcagagtgtc gaaatatctg cgattgaaca gccccgtgag tccgtcagtt
      901 accgccagcg attcgagccg cgcattggcc gtacgcagct cgtcctgcag caccttgagt
      961 cgataatgca cgcgcacgcg cgcgagcagc tccttctcgt tgaagggctt ggtcacgtag
     1021 tcagaagctc cgcgatccag aacttcgacc ttgcgattcg agtcgccctc ggccgtcagc
     1081 atgatcaccg ggatgtgcgc caggtcggcg cgcgtgtggc gcagggccag gaacttcagc
     1141 ccgtcgaata ccggcattac gaggtcacac accactaagt cggggcgcgc gtcgaccatc
     1201 atcttgaatg cttgcagacc gtccccggcc tcgatgaaac gttcgaactc cggaacctgt
     1261 tggagtgcga gccggatctg gctgcgtatc gttggactgt catcgacgag cagaaccagg
     1321 gcgcccatgg ctagaagcgt agcgcggaga atgcagccca tgcgaggcag attgcttcgc
     1381 atcccctgga cgcaaatacc ctacattctc tcgcgcaaga tcggcccggc tcagctcgag
     1441 cctaaaacga tccggtcagg gcaacactgg tgggtcccac tcgcaaccca gccgtagggg
     1501 cctcggcgtc gcgcgccgtg aaccacagca ccgcacctgc agccaacgcc actgcgcccg
     1561 ccgcaaacgc aaccgtggac gcgttgcccg ctgcgcgcgc gtcggatctc agttcgacgc
     1621 cctcatcgtc cgtacacgta gaaccggtgc agtgcttctc ggcgtcgtcg cgtttggaca
     1681 tggacttgag gccaaagaac gtcccgagcc caatgccgac cacgcccacg cctccggccg
     1741 cgaccgctcc gatgcgagcg cccgtccact gaaatgatcc cgggctgacc gcgtcacttg
     1801 gttcgagagc tacgcccgca gcggatgagg cgccctctcc gagctccggc acttgcacgg
     1861 ttttggtcgc gcccttctcc atcacgacat tggtttccca cggctgctta ccgggggcgc
     1921 tcgcggcaat gtggtgggct ccgggatcga ccggaatggg ctggccccac gaagcgggct
     1981 cgagtgtttg cccgtcttgg gtgacacgca ggttcgactg ggtcggcaca atcaccacca
     2041 gcttggggac ttgcggcgcc agcgcctcgg cgcgctcgcg tgctccccga accgcgtccg
     2101 aatagccctc ggattccgcg cgcgcggcgg catccaagaa gcctcgatac gcgctgccca
     2161 cgcggcccac tcgctcgaag caatcggcca aattcaggag ggtaccgagc gccggatcga
     2221 gccgctcact ctgttcgaac ttgggacatg cctccgcgaa cttacccgcg gaaacgagct
     2281 tacgaccctc gttgaacaag gcttcagctt gtgcggcgga tgcgctgggc gtcgccgccg
     2341 gctctggatt ctgggcgacg gcgaaaccag accaagtcgt gcaaaggcca atgacgaacg
     2401 cgctcaacca tctcatgggc tctttccatg accaggtttg cggctctgca tcaagtccgt
     2461 aagcgatgga cgtgatttga cggctggcgt gccggcggtg gaaaccggct tggcaaccgt
     2521 cggagcggga ctcgcaacgg caggcttggc ggggcgaggc tgcgcctttg ccgaggcttg
     2581 agacgcttgc gtgcccttcg gttcacccga agccgccgct cccgcagtcg ccccggccgc
     2641 gccgctcggc gcgctcgtct tcacctccga agcctggctg ctcgcggcgg tttcggcgct
     2701 cgtgggctct ggctcgacgc ggggcactcc cagtccggag cccgtttcag acgcagcctc
     2761 gcgcgggcgg gctcgcagac tgcccacttc gctgctgatt gagccactcg agagtgcgtg
     2821 caccgtcgac gggtgatcgg gagtttcgct atggccccaa agcgcgaacg caccgacccc
     2881 gagtaaggcg acacctgcga ccgacgccac gccgatgccg atgtttcggc gcaacggcag
     2941 atgccgagca ggatgggaat cccacgccga cgcagtgaag gtcgccggac tcgcaccctc
     3001 cgcgcgcgcg gcctcgccgg gcgaagttag cccctgttga ttgccgcgta tgatgcggac
     3061 cgtacgatca atcgagactc gtgcctccgg cggcgccgca tccttgaggg ccacgacgag
     3121 ctcggccaaa ttgctgaatc ggcgctcgcg atccttctcc aagcagcgcg agatcacccg
     3181 atcgaccagt gccggcgcgt ccggccgcac cgtacgcagc gccgggatct cgtcggaaat
     3241 gatcgcgctc atcagatccc acggggtctc gccgggataa ggaggcacgc ccgcaatcag
     3301 ctcgtaaagc acgatgccga gcgaccagat atcggcgcgg gcatctacgt ccttggcatt
     3361 cttgagctgc tcgggcgcca tataacgggg tgatcccatc accgcgttgg tggaagtgac
     3421 gctgacctgg aactcgtcgg tgtcgcacac ggcagctttc gagatgccga aatccaagac
     3481 cttgaccagc ggcgatccgt cagcgcgttg agtcagaaat agattggcgg gcttcacatc
     3541 acgatgaacg atgcccagcg agtgagcttc cgcgatggct tcgcacgcct gcagcacgta
     3601 gctaaccgct tgctcgaccg tcagcggccc tttctctctc aaccaacgcg ccaaatcgga
     3661 gccgcgcagc agctccatca ccatgtaggg ggcaccgaaa tccaaagtgc caacgtccat
     3721 cacgcgagcg acgtgttcac tctggatttg ggcggcggcg cgcgcctccc gcaagaagcg
     3781 ctgggtggcg cccggattcg cggccatcgc gggctgtaaa aacttcagcg caacgtgctg
     3841 cccgaggggc acgtgcaggg cggagacgac cactcccata ccgccctggc ccaggatgcg
     3901 ctccacgcgg tatttaccgc ctgccagcgt ttgcccggga ccgacgaatc cttccatccg
     3961 ggcaggagct taacgtagcc accagactcc gcaactgtgg cttcaccggc gttgccgtca
     4021 cagcggcacc gcggagccgc agccccacga ctgccctcgc ccggggcagc cacgattgcg
     4081 ccagtgtctc gagtcaacgt ttgcggcacc ccggaatcga ggcgccgcat gcgttcagtt
     4141 ggagtaagcc gctggtgaac cgctgctttc agtgacaggc gccagcggca aaagcgccca
     4201 gtagctcgtg gggcgcgtca tcgatcccag aatccaacaa gcagtttccc ggggcgacca
     4261 cgatgcttgc cccaggctga gcctggctcg tcatattgag cgttccgctc aacacgcgga
     4321 cctgctcttt ctcaacctgc aacgccggtt tatcaccctg gaggaagaac cgcatcgagt
     4381 cattcagcat gaacttcggc agatctgcga tctggacggc cagaggtgag aagttcatga
     4441 gcaggctcaa atcgaaactc gagaacgtca ggctcgcatt ttcgccgacc ttctgaacat
     4501 ctacgtcgaa gtggcgaccc gagttgggat tcaggttggc ttcgagcacc gtagtaccct
     4561 cgaacttcag cgtggacggg gcatctccga ggccgagacc caccagcttg agccggtctt
     4621 gagcgccgtc gagggtgacc gtaccctcca aacccgccac cgacaggtcg atgtttccag
     4681 tataggcggg ggctgcgacg tcggaacccg tggccgtagg cgcaggatcg tcgaagcggt
     4741 cgcggaaagc gttgagtggg cctcgcaccc cgaatgcgcc atagtccatt cgcgccgtca
     4801 gtttgcgctc gacgccatcc aggccgagct cgagggtggg tgagctcggc gccagagata
     4861 ccgcgacctt ctcgccgaag tcgttttcga gatcgagagc cagggcctgc agaacgctca
     4921 aagagagagt gtagttgcgc tcggcgttct ttacgatttg tgcctgcagc ttgccgctca
     4981 tgcgattcac cgagccggtg tcttcgccga gcgcatcgag cgccccttgc agctcaccca
     5041 agtccaggtt gagcgccgcg cgcgccggtt cgagctcgag cgtcagaggg ttgcgctgcg
     5101 cggacgtgag caagagtgca acgctgacgc cttgcgcgcc cagcggactc aagcgcagcc
     5161 gtatcgcgag tttctgcgcc tgttccacgc aatccgggtc caagccggta gtctgactag
     5221 gagccgcatc ggtccctccg ggcaccgcga ccgactctgt ttcctcgcac aatactttcg
     5281 gcccgagcaa atacgtggct gcagtcgagg tctcggactc caagttcgcc ggaatgaaga
     5341 tcttctcttt gagctcacga acgagggaat tgatgctgtc gttcagctcg tcatggctgt
     5401 cttggagatc cgagacttcg accttctcgg tctcgcacgt cgtgaccggc tgcgggcagg
     5461 tcgtcgaacc gatcggacag ggcactgccg ccgaggtgga acacacggtg ctgcccgaga
     5521 cggcgctcag ggcttgggcc agactcgcgg actctgcgac gaacccggcc gagttggaga
     5581 gccctcgcag cgtggtctcg gcgttggctt gcgcggtcac cgccaaagat tgtgcagaga
     5641 cattctcgga actctgcggc ccacacgcaa caacccaagg ggccaaacaa acgcccaaaa
     5701 acccaagtgc cgtcgtacgc ttcatgtttt gcatgcctcc accggaacac cctgtgagcc
     5761 ggaacaccct gtaagcttga atctgtttgc cgggacacct gtgcccagca cagtgcagct
     5821 ggcgagcggc agctcgatca gctgcgcggt tggatgccca ccaattcacg cccgactagt
     5881 cagcaagcac cgtgccgctt ctcttcagca cgcctccgac cagctccgcc tcgtgccggg
     5941 gagggatttc ggctcgtgag cgttcacaca accccagaac gacgtgtgac ctgcggtctt
     6001 cagtgtgaat cggtagccgc gacgaatgac ttacggaagc gcgggcggac gcggacgcgc
     6061 ttccagtctg atctcgatgt cacgcgtcac cgcggacgcg tcacaccccc gccaggtccc
     6121 gggcgacgtc cggctgggtg agtgggtcat gttgtgcgcg ttcgacgagg gcgcgctcct
     6181 cgggccctcc aacccggact agtgcgcgcg ctaccgagac gacagcggcg acgagaggcg
     6241 cctcgtcggc ggttgctcga tacagagcga agaaggtctt caagctgggc aattcggccg
     6301 cggtggccaa cgtttccagt gccttcgccg cgtgctcgag atccgcgagc tcggttgcgg
     6361 gatcactcag gtggcgcgcg agcaacggag ccgcacgctt ttcgttcatg gcagcgagag
     6421 cgtctgccag cgggcccacc ggcggcggca gcgcgtccga agccagaaag tcatagcgac
     6481 gctccagcgc ggccaacatg aattccgccc cggcacggcg ctgtgcaagc aactgcctcg
     6541 cgcgtgcgcg cagatccggc gcgagacgcg cgctcgacgc caaatcaatc agggtcttgc
     6601 tgacgctggg gtccggcaat cgcccgagct ccgtcatgag gaatgcttgc gcggcggcca
     6661 tctccggttc gagcgagagc agcgtgcgat cgagctgggc cgccagtgtt cccgctgagt
     6721 tcgttttcgg cagttcgaac tcattggcct cgacgacgca accgcgcact ggcgtcttga
     6781 agtccggtcc atcgagcact cctccctgcg tgccgaagta ggtgaccttg ccgtcagcgc
     6841 cgcacagaac gaacccggag cgcgccgccg acccgcccaa caccgctcct ggcagagctt
     6901 gcacggcacg cagctcggcg gtcttggcat cgaacgacat gaccgcccgg aaataggtag
     6961 cgaggaacgc attggacgcg aaactcacct gttgcccgtc gtaacgcgga ctggcgtaga
     7021 gccggatctt ggcgcgcgcg ctcggatcga cgatcgatac ctcggaaccg ggcccgagcc
     7081 agcgcggctg ccccgggagc acgcgctcgg gcagcgccac ccgcccactc tggcgagtgc
     7141 tcgcgtcgcg gatccgctga tcgaagtgca gcaccgcacg ctcgccaaag aacagctcgc
     7201 ccccggctgt gaaagcgtgg ctcacgacct cacgagtcaa taagcgaccg agctcctcgg
     7261 aattgctcgt gtcgatcgcg ctcacgtact gtccggacca ggggataaag gcgaccccac
     7321 cgagcacagc agggcggccg agtgcgatcg atgtgggcaa gcgggtcttc tcgtgacctg
     7381 cgtgatcgag cgcgagaaac aagctacgtc tgccatcgaa ggtgccgagc gtgacgaccg
     7441 tggtcgaacc gtcatcgccg gcgcccttca acaccatgtc ttccacggag acttggaagc
     7501 gtgacttacc ggagcctgcg tccagtgcgt gcaacacccc tttcgaggtg aacaaaacca
     7561 gatctcctgc gacgctcggg gcgctgtccg gcgcggcggc ataggaccag cgctggcccg
     7621 aagccaaaga cacgccctgc agcgcgcggt cggtgatcgc gagcaccacg gcttgatttc
     7681 gcggacgcgg agcgttgcgc aaggaggctt ccaccgcggc gatcgactgc cccgaatcac
     7741 tgagccaatc ggtggaaaac ggtgtcgaga tctgcgtgct cttggcgcac ccgacgagct
     7801 ccaacacggc aacgcaagca aacaacctgt tcatggcgac ggatctccca cgacgggacg
     7861 cgcgcgcagc gcggcggtca gggccgccct caccgctgcg ctctcttctt tcggcaacga
     7921 agccgagacg gtttcgagca cgctcgcggc gcccttggtc tgcagtcgcc gcaaccgatc
     7981 gatgtaacga attttgttcg catccggtag ctcggcctgc ggccgcagca acaagggcac
     8041 gaagctggct tccaggactt ggaatttgag cttgcccacg aagtccatca atcgatcaca
     8101 ctgttcgggg ctgcacaaac tagcgatcga caccgccgct tctggggtgt cacgcaccaa
     8161 gaccgcgaac aactcgttga cggcttcctt ggcgccgagc tgcccgaggc cagccgcggc
     8221 caaccctcga gtgaccgggt cctgccccgt gagggcctgg cgcagagcgg tcactgcggt
     8281 ggctcctttg gtccgcgcga gcgtgcgcgc ggccgcacgg cgaatttccg tgttacggtg
     8341 cgagacgtat ggcgcgacga agtcgctcga gctggcttgg ccgagcagcg cggcggtatt
     8401 caacgcggaa agcaacacct tggtgtttgc tccgcgcgcc aacaatgaat tgaccatggg
     8461 cgccgcggct cccgcgctct cgcccaggcc actcaactct tcgagcgccg ccagcagctc
     8521 cgactcgttg ctggattcga ggggctttcg ccagcgctcc acatctacct tggcttttgc
     8581 tcccgccgcg gctttgccgg tcgagggtct ggccgcggtg aggcccggct tggccggcgc
     8641 gctgggcttg ggggctgccg gagccgaatt cacggcagga gggttcgatg ctggtgggtt
     8701 tggcgcagca gcggcgagcc cggatgcggt cgacaccaga gcaagccagg gcaagcaacg
     8761 gagcattctg tctcctttgg cgcgagaccg ctcacgttgg cgtgggcggc gccgccaccc
     8821 cgatgtactc tggctcgcct acgcccgcaa gccgctgagc cacaccgagt tcacgatgcc
     8881 atcatcgcct tccctccccg agctgccgtt gattcgattg gagctggtcg aagaccaaag
     8941 tcctcctcac caaactggat ttctgcgcct ggtgcggcgt cgcttccgcg ccatctatcc
     9001 tgacggctca cgcagcgcag agttcaccta cgacgaggtc gatcgcccgg cgatcgatgc
     9061 cgtggtgatc gtggcttatt atctcgacgc gcacggcgaa cggcgagtct tcctgcgctc
     9121 ctccctgcgg ccacccatcg tgctgcgcga tcgaactcgt tcaccgcggc cgggcgcgga
     9181 tcccgtttcg agcatctggg agctcgccgc cgggttggtc gagcaagacg agcagaccga
     9241 gagtggcctc aaaacttccg cgatccgcga actggaagaa gagcttgggt ttcgagccag
     9301 ccccgaccag gtcttcgaac tcggccccag cacctttccc gcaggtgggg tgatcgcgga
     9361 acgtcacttc tatttcggag tacgcgtcga cccgaagtcg cgccaggatc cgggactcga
     9421 tggctccgcg ctcgagcatg gaggcgtggt gaccgatgtc acgttgacgg aggcgctggc
     9481 catgtgccgc gacggtcgca tcgtggacgc gaagaccgaa ctcggcttac gtcgcttggc
     9541 agaaacaccc gttgaacggc ccacatgacc tcagccaagg cgtctaggac gcggaccccg
     9601 accgccacga aggcggccga accacgcatc atcgtggtca ccaaacgtac ctcctatcgg
     9661 cgcttgattc tggaggaacg tgacccgcgc gcgcgaaacc tgatctcgcg caaggacccg
     9721 agcgtttcga gttgggtgcg cgctcatcgt gagcacgagc gcacgctaga gaccgtagaa
     9781 cgagtgctcg gcaagagcgg gctacccgtg ctgtgggttc gccgcgcgca cgccctgttc
     9841 gatacgtcgg gcgcgagcct ggtgatcgcg attggaggtg acggaactct gctggccgcc
     9901 tcccacaacg tggacaccgt gcccctgctc ggcgtcaaca gcgcgcccaa ccatagcgtc
     9961 ggcttcttct gcgccgcgcg acgtgagaac ttcgccgagc tgttcgagca agcgctggag
    10021 cgccggctgc gacacgtgac tctcacccgt atgaaagtgg tcgtgaacgg ccggctccgg
    10081 tcttcccgag tcctcaacga agcgttgtac gcgcacagct cacccgcggc tacttcgcgc
    10141 tacctagtgg agctagatgg cttgcgggaa gatcagcgct ccagcggctt ttggatcggt
    10201 cccgcttccg gatcgacggc cgctcaacgc tcggcgggtg gccgcgtcat gccgctttca
    10261 tccaagcaac tccagctggt ggtgcgcgag ccgtacgcgc catttgatca gaagtaccgc
    10321 ttgctcaaga agcaattctc tccggatcaa acgctgatcg tgcacagtaa gatggatgac
    10381 gccatgctgt ggctcgacgg gcccaatcga cagatcccga ttcggttggg agatatcgtc
    10441 gaattcggtg tatccgatat gccgctcacg gtattgggct tgagctcgaa gcgcactatc
    10501 ctgtgatggg tacgggagca tgaccggtct tctgagaacg cgaatcaaag cgtgctgcat
    10561 cgcgagcatc accgaagctc gaatcgcgat ccaggccggc gcgagcgcga tcggactgat
    10621 gccgcgaatg ccggacgggc caggggtgct ctcggaggca gaggtccgcg agatcgccga
    10681 agccactccc ccgggtgtaa cgtccgtttt gctcacgacg cagttggatg tggatcaaat
    10741 cgttacccag caacgcggct cgatgaccca caccctgcag ctctgcgcgc cgctcacccc
    10801 cgacgcgcgg cttcgtttgc ggcgcgagct gcccggcgtt cgcctgctgc aagtggttca
    10861 catcgagggc gaggaaagcg tggcccaagc gctcgatgtg caacgcaccg ccgacgcgct
    10921 gctgctcgac acgcaaatca agcgggaccg cttcgtacaa ttgggcggaa cggggctgac
    10981 tcacgattgg gccatcagcg agcggatcgt aaaacaagcc aacattcccg tgtttttggc
    11041 gggcgggcta agggctacca acgtcgcgga ggccatccga cgcgtgcggc cgttcggagt
    11101 cgacgtttgc acgggaattc gcgcgccgca cggcttggat cccgaaaagt tggaggcctt
    11161 cgtaacggcc gtggccgaag catcgtgacg tgccagtcgg gagcgcgcac atccgcgaga
    11221 ctccgaggga tgaattttcg actacgcgcc tgcggctccg ctctgtcaca ctccggagat
    11281 gtctgcggag ttccgtagcg gccgatgctt ggcgggttgg attgcggtct tgaccgtcgt
    11341 cctgggagcg tgcgcgcgga atgcacccgc gggaggttcg gccgaaagcg ctccctccgc
    11401 cccgagcgtc acgccggccg cgggctcgat gccttcgatt tcagctcgaa cgagctcgcc
    11461 cgcttctgct tcggccccga actccgatcc cacgcgcgac cccgagcaca cgactcccga
    11521 gccaagctcc gactgcccaa ctgcggcctc gctcggcgcc accgcgctac tcgagctcga
    11581 agccacgacc agtgccgagc aggccgcgat tcaagccgtg cgccgcgaac tgacgcgggt
    11641 tctcgaaaat ccgggaagct actttggaac ggtgcagacc gcgtccgatc tgattttggt
    11701 agagctatgg cacaaatccg ggctctcacc cgaagcaggt tgtcacgcgc cgggcaagcg
    11761 tcgaaccatg actttcgatc cgcgggcggg aaagattgtg gcgacgaaga tctgggagtg
    11821 actcacgagc ccgcggtcgc gatcactcgt tgagaagttt ctcctcgaat ctcagtaatc
    11881 gctcgcaggc gcgctcgaga gtgtcattac ctttggcgaa gcaaaagcgc acgatttgcc
    11941 gcgagctgcc ggcctgatag agccccgaga tcggaatcgc agcgacgcca gcctctcgca
    12001 ccaggcgctc cgcgaaagct tgatccggca ggcgggagag cgccgagtaa tcggcgagct
    12061 ggaagtacgt gccgcgcgta ctcggaaact ggaggcggct cgagcttaga aggcttcgaa
    12121 acaagtcgcg tttacgctga tagaagccgc tcagctcgtg cagatgctcg ggagcgctca
    12181 ccaggacctc cgccaaggcg tgctgggcgg gcgtgttagt gctgaaagtc acgaattggt
    12241 gcaccttgcg gacttcttcg gtgagcgcgc ggggtgccgc gatccagccc accttccagc
    12301 ccgtggcgtg ataggccttg ccgaacgagc caacttgtaa gctgcgttcg gcaagctccg
    12361 ggtgccggag cacgctttga tgggcaccgc cgtcgaacac gatgtgctcg tacacttcgt
    12421 ccgagagcaa ccaacaggac gtgtcacgca gcaggcccgc caagcggtcc agatcttccg
    12481 cgccgagcac ggccccactc gggttatgcg ggaaattgat gacgagcaag cgcgtgcgcg
    12541 gcgtcaacgc agcccgcaga cgctcgaaat cgatcgagaa gtgttctgcg tcgagcggaa
    12601 cgtgccgcgc ccgtgcgcct tgtagcgcga cggcagggcc atacgagtcg tagctcggat
    12661 cgagcagaat cacctcgtcg tcagcgccaa ccagcgccgc caccacgtcg aagatcgctt
    12721 ctgtcgcgcc gctggtgatc gtgatctccg actctggatc gagtgctcgg ccgtagtctc
    12781 ggcgcaactt ggaggcaatt tgctctctca gtgccggtat gccggccatg ggcgcgtatt
    12841 gatttctgcc ctgcgcagcg cagcgcgcga gggcttcgag caaggccgcg ggcggctcga
    12901 aatccgggaa accctggccg agattcaagg ccccgtactg ctcggcgaga cgcgacataa
    12961 cggtgaagat ggtcgtcccg acctgcggca gcttggagtg gattcgcatg cttcctcgcc
    13021 cgcgagcttg tcacgaaccg cgcccagccg ggaggccctc gacgcgcccg gtggcccgtg
    13081 ccagcgtgcg cgcatggcca ctgccagcgt gctgtcgatc ggaaccgaaa ttacccgggg
    13141 cgagctcgtt aacaccaacg cgagctactt ggcggagggc ctcaccgcgc gcggcttcga
    13201 ggtcaccgag atttgtacgg tagacgacga cgctccgcgt atcgaggcgt gcctggcgcg
    13261 tctggggggc agctgcgatg tgatcgtgag caccggaggc ttgggcccaa ctacggacga
    13321 catcaccacc gagtgcgtgg cgcggctgct gggagtgcct ttgcgcaggg acccggagtc
    13381 gtttgcgatc ctcgagcaac cgctgcgcga tttcggacgc agcgtgtcgg ccagcaatgc
    13441 caagcaaact gacttccccg taggcgccag tgtgttgccc aacccgaacg ggagtgctcc
    13501 cggcttctgc gtgcgcatcg cccgcgctct agcgttcttc atgccgggcg tgccgcggga
    13561 aatgaagccc atgttcgaaa gcagcgtgct cccggcagtg gcgccgctgg tggatgtcgc
    13621 gagtcaccag atccatctgc agacctttgg tctacccgaa tcggaggcga atgatcgctt
    13681 ggcgggcgtg gaggctcagt tcggtgtgct gattggatac cgagtgagca tgcccgtgct
    13741 cgagatcaag gtgctggcgc gcgatgccag tgcgagcgtc gcggagacac gcgcgcgcac
    13801 ggccgcggac gaggtgcgac gccgcctggg cgatgtggtc tatgccgagg gtcacgccac
    13861 cctacccgag gtgctcgggg cggagctcaa gtctcggggg ctgcgcctgg cttgcgcgga
    13921 gtcctgtacg ggcgggttag tcggagagct gctgacggat cgtccgtccg cgagcgagtt
    13981 cttcgcgggg agcgcggtgg tctacgagaa ccggcagaag acccggttgc taggggtgga
    14041 cgcggccttg atcgaacagc acggggcggt cagtgaaccc gttgcgcgag ccatggccga
    14101 gggcgctttg cgcgtgttcg aggtggacgt cgcgctcgcc atcaccggta tcgcgggtcc
    14161 aggcggaggc accgagacga agccggtcgg gctggtgcac tatgcggtag ccacgccgcg
    14221 cggcaccacg gcccgaagct tcgtgttcgt acgggaccgc tacatggtga ggcgccgagc
    14281 cgccttcgcg gcgctggacc tcgctcaccg cgtgatccgg caggggcatc cgtaacttct
    14341 cgttccgcac gcgaagcaag cacgcgcacc gccacgctgc tggcgtccga tcgggcgcgg
    14401 ctaacgatga ccgagaacgc gcagcagttc gcgagccacg tcgggagaga gggtaccgcg
    14461 ggttacccga cgttgaacgc gttgccaatc ctcgtcactc actcgaagcg ccaacgcttg
    14521 ctcgcgatct gcagcctcca acgattggcc cgggcggctc acctcggcga atcccgcagt
    14581 cgcgacgcgc acgggccgag aggcgagccc cgcggtgaca cgcggaggca gccggggatc
    14641 ccaaatccgc gcagattcac gcacgcttac gctgccgaga tcggccgaca acttgagtac
    14701 ggcatccgcc gtcggtgtgg aagggaacgc aatgctcgcg ctacccatcg ccgtgcgtgc
    14761 ttcaatcgcg accggcaagc cgcgtggcaa atcgacgcgc gcagtaccca aattggtttg
    14821 cacccggtgt tcgccgggag cgaggcttac gacctccaac cgaacggcac cggcgttcga
    14881 tgatacgtcg atggtgcctt cgagctcggt gccttcgatc cgccccgcgc tcgccgcgat
    14941 cctcaaattg cctcgaaaag agtggatgtc gatgcggcct gcgtccgatc ggatgttgag
    15001 accaccgctc aggtgctcgg cggaaatccg ccccgcgctc gcggtaacgt cgagcagggc
    15061 tccatcggga atgtgcaaac gcagctcgcc gtgtgacgat agaaactctc ggaaaggcca
    15121 gggcaactcg tgattcgccg aacccaaggc gagccgtacc gcattcggat tgcgatcgat
    15181 gcgcaatcgc tgacgcaaca cggggtggtc gccgccaatg taaaaacgat cggggcgtcc
    15241 gctcggtagc agccgcaagc gcaggaagtc cgcgctcacg acgatggacg caccggggtt
    15301 gacggcgatt tcttcttcga acgcgtctct cttcactggg ttgcctgttc tgtttggcgc
    15361 tggtgaggat ctggggcggg tccggtcggc ggcaacccgg gacgggtgcg ttgcggtgtg
    15421 tagggaagtt gctcccttgg cttttgggcc actaggctgt gctcgtgcag catgggatct
    15481 gacgaaaacc ccaaagaccg gccacccgat tcgctggcgg acggcgccga gactccacgt
    15541 cgaatcggca attaccgagt cattcggcag ctcggttatg gcgctttcgc ggacgtctac
    15601 ctggctcagc acgaacagac gcacctgcaa gtcgcgttga aggtactgcg ggcgcagctc
    15661 gatccgcgat tggcggagca aatccggctc agattcctgg cggaggagcg gatcgcgaga
    15721 acgatcgacg acgcgcggat cgtacgcatc tacgagacca gtaccggtga agaagcaggg
    15781 ccgtgcttca tcgcaatgga gtacgtggac ggcaaaccgt tctgtgaggc atttcgtgga
    15841 ttcgagccgc tcacggacga ggagcgtcaa ctcaagcttt cgcaactcgg aatgatggtc
    15901 gcgcaagcgg tcgcgcgcgc ccacgagcgc ggcatcgtgc accgcgattt gaaacccgac
    15961 aatgtgctcg tgacgcgcgg accgcggcac ggcattaaga ttctcgactt tggtatcgcc
    16021 aaggctccta tctcgctctt ggcgagcttg cccaagccag cggggagcct aacgtcgcag
    16081 ctcgtcaccg agcttggcac ggtcatgggc tcgcgcccat atatggctcc ggagcagaat
    16141 ggggccgcgc acgcggtgac cggcaaagcc gatgtttatg cgctcggcgt gatgctcgcg
    16201 atcaccgccg cgggcttgga tgcggacgcg ctcgagacga gcggagcctc ttgggtttta
    16261 caggaagacg tggatcgcgt gatcgatacg cgcccgctct cgaacgagtt ttcgcagctc
    16321 ctacggcgca tgttggagct cgaacccgag gcccgacccg agatggccga ggtcgcgctc
    16381 gagctccaac gctggtcacg accggagcgt gcattcgcgg acgcggtcta tgcctttcgc
    16441 aagcacgcga aaatgccgac gaaaggagcc ctactccgcg ttctgcgcga cgtggagcat
    16501 gaggctcact tcacggagga cgagctcgag ttcttgagac tagtccctgc ccgcagcttg
    16561 gcgagcacgg gggcggggct gccttgggcg ttggtaggcc tatgcgctgc gtcggcggcg
    16621 gcgctcgggg taacgtacgt tcgacaacgc gaacaagcgc agagtgacca agccaagctg
    16681 cgtgttcaga atgccgacgt tctcaaattg tatgcggatc tcaagcaaag cgtggggcaa
    16741 cccggcgaga ccgggacggc cgacgcccgc gaactccggc tcgccagcgc ggccgctccc
    16801 aaatcggatg ccaaagcgca ggagcgctcg gcgcccgtat gccgcaacaa cgccgagcag
    16861 ctcgccgaat gcgtcgagag acgacggggg gccgagcgac aagccgccga atgcagcggg
    16921 cggtcggaaa acttggcgca gaatctagag acgcagcgtc gcgagctcga cgctcatgtc
    16981 caggccgaag cgcagtgccg gcgcgagcta gatacacgca tcgcgcaact cgcagagtgc
    17041 acgagccgag tgcgactgtg tagcgagagc ttgcgcgcca cgaactcggt gcgcacggtt
    17101 ccggccgatc cggacggtgc cgtcgaatga gcgctcgctt tccttcccga ctgggatgct
    17161 gcctcggggc ggttctgatg gcttgtgggt cggcggaaca tcgggtctca tcagcgccac
    17221 gaactggagc tccgggagca acgccacctc ccgtcgcggg tgaatccggt acattctcca
    17281 gcgttgaccg cttgcctcga cgcgaaggtg ccgcggcgat cgcgaacgcg acaccaccct
    17341 cctcaccgcg tggatcgcac gagaatcctg atccggagcg gcccccgctc agcgggcccg
    17401 agacggggag tacgccccct gagctgccca gagcggccat ggtgttgcag gtaggtgact
    17461 ccttcgcaga cgctctcagt aaacccttga aggctcgact cctcgccgag ggtttacgca
    17521 ccgcggtgga ggtcaaaacc gcaagctaca tcggtacctg ggcctacgga gcggacctcc
    17581 cgcggttcgt ggctcgctac aagcccgatt tgatactcgt gacgctaggc gccaacgaac
    17641 tctccattcc ggagccggaa gcacggcaaa gcgcgattcg acggctcgtt ggcagcatca
    17701 gctcgcaccc gtgcgtgtgg atcactccgc cccgttggaa gaaggacact ggggtgctgg
    17761 aggtgatccg tcgcaacgcg gcaccgtgcc gcttcttgga tagtgatcag ctggtaggcg
    17821 agttgcctcg gggcccggac aagattcatc ccaccaagca gggttccgag cgctgggctg
    17881 agctagtgct cggctggttg cggcatgaac gagacccttc gggtgccacg cccttcgcgc
    17941 tcaaaccaga gccgctccag ccgggagcgc cgagggtcga gacgtcccaa agcgcgacac
    18001 acgatccgag ccggcaatag aacacccgta caactttcat ccacgctgag cgcaaccaga
    18061 ccgggtgaac caggcgggct caacaaccgc ccaggacgac gaggcggagt gaacgtcacg
    18121 agacggcggt ttcggtttgc gcgtacccag agtctggacg atggttaagc caaccgttcc
    18181 ggatgagcag ccgggcggtt ccttcgagcc tcctcatgaa tcgcgtggcg agccccctgc
    18241 ccctgagcca tccccccgac gaactgcgcg gcttgaagct gagtttccgt ccaaacctgg
    18301 agttgatcgc tagcactcga cgtttggtcg aagacttgct ggacgtcgag ctcacggatc
    18361 cggatgcccg atcgcggatc gtggtggcca ttcacgagct gatcgagaac acgtacaagt
    18421 actcttccga cggcgcggcc acactcgacg tcagctttgc tcgcagcaac gcgggaggca
    18481 cgcgggtttg tatcctggct tccaatcgca cgttgcccgg acgcgccaag gaattgcagg
    18541 agcggatctc gtcgctcggc cgcctctccg atccgatgag cgcttacgtg cgatggatga
    18601 cggagagcgc acagcgcgaa ggctctgggc tggggctgct gcggattcgc gctgagtgtg
    18661 aaatgcaact ctcggctgcg ctggacggcg atcgcatcac gatcgaagcc accgcgctcg
    18721 tctcctgacg tgctcgtgaa acccgcgtcg agcgcaagag agctgagctc gatgcgcaac
    18781 gttctacgcg aacttcagag cttccgacag taaaggagca ccaccgaggg gggcgtcaag
    18841 ttgatcgctt gaccctctcc agtgtcgttg gtcgtgaagg tgtgcgtgtg atccgacatt
    18901 ggccaggcgg tgtcgttgcg ctccgtgtag gcgccactac cgaggccaat cgatcgattc
    18961 gtgaagtagc tctcccctgc cgagtagtac acgcgatagt tcatgcgcgc acctcgatct
    19021 gtcgcaccgg tgtgatgatg cgcgggcagc tcctccaccg tcagcgtgtg ggactcctcg
    19081 ccaaagcgct caccacgcac tcggcgtgag agtttgttgg agttcgcact atccggatct
    19141 accggattcg tgccaacgat tgttcgaccg cgcgccggtt cgaaggcagc ccaacccgcg
    19201 ggacagactt cgagatcgaa aggcatgacc gcgcccgagg gcacgatttg ctgggcgagt
    19261 tccccgaccg ctgcgcccgc ttcgtctgca tacgccgaac gcaatgcgaa cggaacgctc
    19321 gtgatttttt ggcgcggctt cagttcttcg tcgtcagcga tccgaatccc gagatagaga
    19381 ttgccctcgc tctcgaacag ccctgcggga aagggcgtct cttcgccgag cctcacggag
    19441 aagtagccat tctcgaccgc cacggtgctg cgctccaacc agaggcttcg tgaggctatc
//
LOCUS       DASTKU010000732        14274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1482796, whole genome shotgun sequence.
ACCESSION   DASTKU010000732 DASTKU010000000
VERSION     DASTKU010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14274
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482796"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..551)
                     /locus_tag="VFQ61_25705"
     CDS             complement(216..551)
                     /locus_tag="VFQ61_25705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957930.1"
                     /translation="MQKAQLETIARRTISNVSWGFGNVESTKALLKRELDALGEEDNA
                     RRAQTLLRFGIIDSNPDGQAAVFAAACAAESSICEPQKMKDAALSETRARHVSPGDVL
                     PLYFIGGHP"
     gene            899..1636
                     /locus_tag="VFQ61_25710"
     CDS             899..1636
                     /locus_tag="VFQ61_25710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957931.1"
                     /translation="MINTRSTLVWIAFSAGVAALSSACGTSSSSPNTAGATGLPPAAG
                     GSANAGQAGASGGLTPSGGTSTSVNGGSTASGAGSGGAAAGSSAVTGGAAQSGGAAQS
                     GGAAQSGGAAQSGGTAQSGGTAQPGGSSSAGMTGAGNATFTAVAGIIESRCGAPQCHG
                     GSPEGRSLNLVDRSKLYDRLLNTTVSACSNHPLVTPNDPNSSALIDLPTWQCKDIVMP
                     QGCFDDPCLDAQELNTFRSWISAGAPK"
     gene            1894..4746
                     /locus_tag="VFQ61_25715"
     CDS             1894..4746
                     /locus_tag="VFQ61_25715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957932.1"
                     /translation="MAQARNSRLGDHWAIQVAALMIAAGALSACGSEGASGGPGAGSG
                     GSGATSGGGSGAKPGQGGSSGASTTGGTVTTGAEGGVGNVAGEVGDPNSALAAKIELP
                     IPREGQVLTASTLANQLTHYDPGSLLRCSVAEDAKYRKAIDALGGVSWGYNITALEDS
                     SKPRYLAGVERIWNDFADSPASGASPPVEIVAPDIVAVTDAAALFHSSTHGLMIVGLD
                     QLDKGKPEFRCAVPLPGIVDQFFFHGGELVVMTKSHDGRASHLLHFKVQGSELQFVED
                     VDLGAVSILDSRRFNDKLVFYTSFSPRQVSDMVPSPDPIGGSSDEAASQGAAPTSPLS
                     PRALERHRALRVFALGDKLEEELYDTLLDTSEPEAQLVHERITREMPIGTLVSESRSF
                     GQSMWASDHYFVVTEQIAKTSVEGWRTLSYSSCTQSHTVPSRYEYCETQYETRPNPDY
                     VAPDNSGGDRACHGTTLSDCLIEVARVANKTIQVPVGRKCEEREYQNWVCDHYELVTN
                     EYPELRTDISTRLYIYEYTESGFVRVDSSVHEVNAEELDTLAADATVKSVRTSAETYD
                     LAVPGGIQTLYFQNGFLYVVSQGVLQVYAMGGGSIVRTSTLPVVNETLQSSLFSKDKL
                     ILSDFGWASGDHSTLRVINLSNPAFPKQDGATHELPGGHRSIIAADSGIFTIGSVQSF
                     MGQQVSRIKLGLFSDPYAEETAYSILGTDLKWTWLQAEETQFFNAGSQRLALPYAGQN
                     EDGMPLQRVGFSRVVPGSIVSEGAVVVPEAVQRVRPIGTQDETYLTFANSSVEWVLPN
                     DREWRSEPVLEYLEPFAIYRQNADAEYVELQRLGTRCRLYFAQETTINDREHGLYSQE
                     FACVGYTEAYDKRFLFGERLGIEYDDEHEIRALSPEEVSDTLAEIRARPVCLQRLELA
                     DDVNLDPKSPPAVEDFTCMSPEEYGALRNRLSQRNN"
     gene            5022..5783
                     /locus_tag="VFQ61_25720"
     CDS             5022..5783
                     /locus_tag="VFQ61_25720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9957933.1"
                     /translation="MAKFSRERGGAGAPAARADNAARAARENRENNAQRDTERPTWLE
                     LRYRGRTLMFEVTEGARPLTLGAGLCCDLRVDGAGVALVQFELARQSGKVWLVPKPAA
                     HVRVNAAHVGQPRELSTRSVIEFLEHEIEVLLHARFPGQSSAGLPSAGASRVSAENAF
                     AREGTASGPARDEPAPVTVRAFGNLAGLVERSPTSSRGSVLEEAYAPVEVAARTLPTP
                     AFPPPPTQLAPECGTEGGAGTWLAMPAQRRTLDGR"
     gene            complement(6001..6408)
                     /locus_tag="VFQ61_25725"
     CDS             complement(6001..6408)
                     /locus_tag="VFQ61_25725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957934.1"
                     /translation="MSRYIGQSGRSVVVVLDELPPLDCVMPPSAPEVPPPECIAPPEC
                     IAPPECIAPPEFVVPPSVVELPPGFGADVPPDDAPPAFEGEAPALAPLEAPLLPAAPP
                     KFPLPSPELEHAAATIQTTQYPSNPSKLQGLEG"
     gene            6613..7983
                     /locus_tag="VFQ61_25730"
     CDS             6613..7983
                     /locus_tag="VFQ61_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET9957935.1"
                     /translation="MNCQSEVEPGSVSGALERALSVLRTVVDIVETPVDGPEPPEWCV
                     RRGWAPRLLALSESELASCEERGLGAVVPQLASCPAEWLELSSVLRACTSLPRLASVK
                     AELPSRALRGVSARKREQLAEFIPNVAALVRRSERVVDVGAGAGHLSRLVAELFQRDA
                     WALDRDAQRLERGRSRCQDRAREVGELNVRFVEAHVGEHELEFAPNDLALGLHACGEL
                     GDRLVQQAARVRCHVGLVSCCLQKTQAERRMSMARAARGFTLRRSALGLANLTARAEG
                     VEATLAENLRAREARLALRYLLCERGIELKPGEEMRGINRRRAVAGFAELSAHALAVL
                     GLPAASPAELRAHVERARRDYAVMRRLSLPRNWLSRLVEVAIVLDRGARLEEAGLHVR
                     VAQLFEQRVTPRNTVLFASRDAQLLPPLSGESARGLDASDATPKRTFLVGTANMHNRD
                     QDASSG"
     gene            7967..8917
                     /locus_tag="VFQ61_25735"
     CDS             7967..8917
                     /locus_tag="VFQ61_25735"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET9957936.1"
                     /translation="MPAQDNRAEPTASVERPTGLGPILLVDTYSLVFRAHHALPAMNT
                     QAGEPTSALYGFSTVILKLLKEQRPAALAFALDAPERTFRHEQYAPYKGTRERAPSEL
                     VAQLRKLPEFLPAFGVPVHCAPGFEADDVIATLASEFEPRGRQTLIVSGDRDLLQLVT
                     PHTRVWFVGARGQKPTLFDQEQVLQRFGVEPAQLPMRAALVGDTSDNLPGVPGVGPGT
                     AAKLVREFGTVEALVRGLGSVKPSRVQEALASHVQQIVLNEKLCRLVRDVPLSGAPPA
                     AALSAQSFARLRQAFESLEFRSLVPRLDAIERITLETRAV"
     gene            complement(8892..10343)
                     /locus_tag="VFQ61_25740"
     CDS             complement(8892..10343)
                     /locus_tag="VFQ61_25740"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9957937.1"
                     /translation="MHSEVVELLAKGGLDESTARCWFPSSTGEVFEAYAQALSHGRKL
                     TLVGAKKSFGGHFLCPPGADALDVRDLGEPATLVEREPDGAIWVKVPAGVTFRQLLAQ
                     YPEYQPRSPPTSDGISISGALASCTHNMAGFFADGVRAFALLSPDGRSHRCSPRGNAL
                     EQALFQFAPGTFGALGVFTDIELRLERVPPDFALCIEVASCGKVREPQCYEELFRIAA
                     DPKHAPGHGLFFYGLRDRYVLVADSIASNRASRRLTEAPLTGDRPRFYAALQGLSNRH
                     PALARHIALGAFRPGRAFSADWYGFLFFQRSYDTAYDILSSRSLTFAGLRALGVDPRL
                     TVCHQTWCFPREHVAEFMSVYWEVMQRYAGVERRAEQQDLILLPVCRWPAHGTWGNTQ
                     GSAYITPSIAVRRGSAGHTRAAAFYSELSRVAFERNPRFKVLLLKQSTCDDGLLREMH
                     ADYVARLRELKRQVDPEQLLTSKLLSRLLVSPA"
     gene            complement(10394..11671)
                     /locus_tag="VFQ61_25745"
     CDS             complement(10394..11671)
                     /locus_tag="VFQ61_25745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HET9957938.1"
                     /translation="MTLRALENGVSGLRAEGEALGVVGDNIANVNTTGFKRQRSIFED
                     VLLKSGSGNGSGAGVENSRTQQAFTQGALTQTGIATDLALNGDGFFVVTGATNGVTGA
                     FYTRSGQFKIDASGYLVNPEGLKLVGRPAKSDGTLAAGVSSLQVPTAGIPARATANIQ
                     LAANLDASAPAATFDPLAPSATANASTSIQIYDSLGAAHSLDIYFEKVGDGQWNYHAF
                     LPGSDLDPVVAGPTEVGAGSLTFNSNGALDSVATNQAISVSFGGGAIAGQTVALEFGS
                     TIADGGTGLDGTTQFSMPSSTSSESQDGYASGALSGVSIGSTGVVEGLYSNGLRLPVG
                     QLVVAKFRSQEGLGRAGQGLWVATAESGDPALGAPGSGGRGSLSSGALEASNVDLGEE
                     FVGMITHQRAYSANSKVIATADDMLSMLMQMKR"
     gene            complement(11726..12502)
                     /locus_tag="VFQ61_25750"
     CDS             complement(11726..12502)
                     /locus_tag="VFQ61_25750"
                     /inference="COORDINATES: protein motif:HMM:NF005437.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9957939.1"
                     /translation="MKVFITGASSGIGAAMAREYAKGGARLGLVARRADALERLASEL
                     REAGAKVHVYAADVTDASAMRVALDDFAATAAGGASSIDRVIANAGIGIGDTLRSGDA
                     EAVAQLIRVNVIGVTNTLVPAIPMMLKQGTGTLVAVSSVAGFRGLPGRAAYSASKAAI
                     LTFMEALRMQLVGTGVHAMTLCPGFVRTPLTATLTHKLPFLMEVDEAARDMVQAVEQR
                     KATFTLPWQMNLLRHVVAHAPEWLVRRMAPPARSQGAMSG"
     gene            12580..12924
                     /locus_tag="VFQ61_25755"
     CDS             12580..12924
                     /locus_tag="VFQ61_25755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957940.1"
                     /translation="MPRKRWSEALSYSLGAECAVWGISRFKRANLLVYSSSRVVRLCN
                     AYSMQAIRSRPKLVERFAPRAPALFLVILAFQLNGCEAVKGIFKAGFWVGALFVIGIL
                     GLVAYLLGRKRS"
     gene            complement(12928..>14274)
                     /locus_tag="VFQ61_25760"
     CDS             complement(12928..>14274)
                     /locus_tag="VFQ61_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9957941.1"
                     /translation="IRLELIYQKLEQSGEWTGELRHTTRDGRELIVESRQQLVRAYGQ
                     VWVLESNRDITERKRAEAELRDADRRKDQFIAMLAHELRNPLAPVRNAVEILKRSGTD
                     GPQLSRARDVIARQVTHMTRLVDDLLDVSRIARGKLELQKQPCDLRGIVHQTADDYRA
                     TLEVAGLTLTIDEGTEPLWVEGDPVRLAQMVGNLLHNASRFTPSGGRVRVRTSRDTGS
                     RQAVVSVADTGTGMEPSFLSRLFDPFSQADQDFARSKGGLGLGLALTRGLVELHGGRV
                     EAHSPGPGQGSTFVLRVPLSESRQRMEPSSSTPAARDRPLRILVVEDNADAAETLGEL
                     LTLQGHQVKLALDGAAGVALAREFRPDVVISDLGLPGEFDGYAVARSLRREPSLSRIH
                     LIALSGYASDDARRRSSEAGFQAHFAKPPDLPKLEQTLNAVASSLDSDRHASAPRA"
BASE COUNT         2579 a         4334 c         4739 g         2622 t
ORIGIN      
        1 cgtcgagcgc aacgatcgta cgacgcgctg gtggttgtcc gcgccagcct atgacgtcga
       61 tgcacaccac acgaccgcgc gaagagcgtg cggctgcgcg cgctgaatca acggcatacc
      121 gacggcgccc aaatccgtca aagccgctaa ctaaattgac ccttttcggg ttgcgcgtga
      181 tcggtttgat tccgggggtt cgcgtgaaat tcaggttaag gatgcccccc gataaaatag
      241 agcggcaaga catcacctgg gctaacgtgc cgggcgcgtg tctcgctcag cgccgcgtcc
      301 ttcattttct gtggttcaca aatcgaggat tccgcggcgc aagcggccgc aaatacagcc
      361 gcctgaccgt ccggattcga gtcgatgata ccaaatcgca ggagcgtttg ggcccgtcga
      421 gcgttatcct cctcgcctaa ggcgtcgagt tcgcgtttca acagcgcctt cgtcgattcg
      481 acgtttccga atccccaact cacgttggag atcgtgcgcc gagcgatcgt ctcgagttgc
      541 gccttttgca tttccggaat cgaggttgcc aatcgcgtaa agttgtcgtg tggctgagtg
      601 tgccggagat gcccgaggcc catcgccacg gctgcgattc cgagcggccc gactccccat
      661 ctgatgtacg agcgccatcc gttggatgaa cgcgattcga cgagtgagct attcaacgct
      721 gagcctttct ccaagcatga gctggtgagc gttccccgag atgttccgcg tcgcgaggcc
      781 tagcacgtgg tcaccgacgg tactatgttc gggttcaccg acggtgctcg ggctcgagtt
      841 ctcccggatg gatattggtg gccgttcgag attggcaacc ttcgaaagca gatcaatcgt
      901 gatcaacaca cgttctacac tcgtttggat cgcattcagc gctggggtcg cagcgctttc
      961 ctccgcgtgc ggtacgagct cgagttcgcc gaacaccgcg ggcgcaaccg ggctccctcc
     1021 tgcggcaggc ggatccgcga acgcgggtca agcgggcgct tccggtgggc taacgcctag
     1081 cgggggaacc agcacgtccg tcaacggagg aagcaccgcg agcggagcgg gctccggcgg
     1141 cgcggcggct ggttcatccg cagttactgg cggtgcggca cagagcggcg gcgcagcaca
     1201 gagcggcggc gcagcccaga gcggcggcgc agcccagagc ggcggcacag cccagagcgg
     1261 cggcacagca cagcctggtg gcagcagctc cgctggcatg accggggcag gaaacgcgac
     1321 cttcaccgca gtcgcgggca tcatcgagag ccgctgcggt gcgcctcagt gtcacggggg
     1381 gagcccggaa gggcgttcgc tcaacttggt ggaccgtagc aagctctacg atcgcttgct
     1441 gaacacgacc gtttcggcct gcagcaacca tccgctcgtt acgccgaacg acccgaatag
     1501 cagtgcgttg atcgacctgc cgacgtggca gtgcaaggac atagtgatgc cgcaggggtg
     1561 tttcgatgat ccctgcctgg atgctcaaga gctgaatacc ttccgcagtt ggatttctgc
     1621 gggcgcgccg aagtagctga cgtggagtga gcgagcctcg aaggcctgcc ccagctcggc
     1681 acaaagtggc gcatcgagtt ggcgcaatcc gcttagcagc gggcgcgaga agctgcgcct
     1741 gctgctaagc gcaggaattt gtgcggcgcg aagcaagcgg tttaggtgcg cccgggtgcg
     1801 tgctcgctcg gcctgctcct tgctaagcct ttcagatagg agccagcggc aggctgcgct
     1861 ccggctgaac gggtttggag cggacggcaa gagatggcac aagcacggaa ctcgagattg
     1921 ggtgatcatt gggcgatcca ggtggcagcg ctgatgattg ccgcaggtgc cctgagtgcc
     1981 tgtggctccg agggagcttc cggtggtccc ggggcgggct ccggtgggag cggcgcaacg
     2041 agtggcggcg ggagcggcgc aaaacctggt cagggtggct cgtccggcgc ttcgacaacg
     2101 ggcggaaccg taaccacggg ggctgaaggt ggggtcggca acgtggcggg cgaagtcggg
     2161 gatcctaact ccgcattggc cgccaagatc gagttaccca tccctcgaga agggcaagtt
     2221 ctgacggcct ccacgctcgc gaaccaactc acccactatg accccggcag cttgctgcgc
     2281 tgtagcgtgg cggaggacgc caagtatcgg aaggccatcg acgcgttggg tggcgtgagc
     2341 tggggataca acatcaccgc gctcgaggac agcagcaagc cgcgctatct ggccggggtc
     2401 gagaggatct ggaacgactt cgcggattca ccggcctccg gtgctagtcc tccggtggag
     2461 attgttgcgc cggatatcgt ggccgtcacg gatgccgcgg cgctattcca ctcgagcaca
     2521 cacgggttga tgatcgtcgg tctcgatcag ctcgataagg gaaagccaga gttccgttgc
     2581 gcggtgccac tgccggggat tgtggatcag ttcttcttcc atggcggaga attggtcgtg
     2641 atgaccaaga gccacgatgg gcgcgcgtcg catctgctgc acttcaaggt gcaaggcagt
     2701 gagctccagt tcgtcgaaga cgtggatctc ggagcggtga gcatcttgga ttcacggcgc
     2761 ttcaacgaca agctcgtttt ctatacgagc ttcagtccac gacaggtgag tgacatggtg
     2821 ccgagcccgg acccaatagg agggtcgagt gatgaggccg cctcccaagg cgcggcgccc
     2881 acttcaccgc tctcaccacg ggctctcgag cgccaccggg ctctgcgggt gttcgcgttg
     2941 ggcgataagc tcgaggagga gctctacgat acgctgctgg acacgagtga gccggaggct
     3001 cagctcgttc acgagcggat cacgcgcgaa atgccgatcg gcacgttggt gagtgaatca
     3061 cgcagcttcg gccagagcat gtgggccagt gaccactact tcgtggtaac cgaacaaatc
     3121 gcgaagacct ccgtggaggg ttggcgcacg ctgagttact cgagctgtac gcagtctcac
     3181 accgtaccga gccgctacga atattgcgag acgcaatacg agacgcggcc gaatccggat
     3241 tacgtggctc ccgacaactc cggaggggac cgcgcctgcc acggcaccac cttgtccgac
     3301 tgtttgatcg aggtggcgcg ggtcgccaac aagacgattc aggtgccggt cgggcggaag
     3361 tgcgaagaac gggagtacca gaattgggtt tgcgatcact acgagctggt gaccaacgag
     3421 tatccggagc tgcggacgga tatctcgacc aggctctaca tctacgagta tacggagagc
     3481 ggcttcgtgc gggtggacag cagcgtgcac gaggtgaatg ccgaagagct agatacactc
     3541 gccgcggacg ctacggtgaa aagcgtcaga acctccgcgg agacctacga cctggccgtg
     3601 cccggaggaa tccaaactct atatttccag aatgggttct tgtacgtcgt gtcgcagggg
     3661 gtgctgcagg tctatgccat gggcggcggc tcgatcgtgc gtacgtcgac gctgccggtc
     3721 gtcaacgaga cgctgcagtc ttcgctgttt tcgaaggaca agctgatctt gagcgacttt
     3781 gggtgggcga gcggtgacca ctccacgttg cgtgtgatta acctctccaa tccggccttt
     3841 cccaagcagg atggcgccac ccacgagtta ccgggcggtc atcgatcgat catcgctgcc
     3901 gattcaggca tcttcacgat cggcagcgtg cagagcttca tgggtcagca agtgagccgg
     3961 atcaaactcg ggctgttctc cgacccgtat gcggaggaga ctgcttactc gatcctgggc
     4021 actgatctga agtggacttg gctgcaggcc gaggaaactc aattcttcaa cgccggctcg
     4081 cagcgcctcg cattgcccta cgccggtcaa aatgaagatg gtatgcctct ccagcgcgtg
     4141 gggttctcgc gcgtcgttcc aggctccatc gtcagtgaag gggcagtcgt agtcccagag
     4201 gcggtgcagc gcgtgcgtcc aatcgggacg caagacgaga cttacctcac gtttgccaac
     4261 agttcggtgg aatgggtgct gccgaacgat cgcgagtggc gctccgagcc ggtgctcgag
     4321 tatctggagc cgttcgcaat ttatcggcaa aacgccgatg ctgagtatgt cgagttgcag
     4381 cgtctcggga cgcgatgccg cctctatttc gcgcaagaga ccacgatcaa cgatcgggaa
     4441 cacgggctgt acagccagga gttcgcgtgt gtaggttaca cggaagcgta tgacaagcgc
     4501 ttcctcttcg gggagcgcct cgggatcgaa tacgacgatg aacacgagat tcgagcccta
     4561 tcgcccgagg aagtgagcga tacactggcg gagataagag ccaggccggt ttgcctccag
     4621 cggctggagt tggcagatga cgtgaacctc gacccgaaga gcccgcccgc cgtggaggac
     4681 ttcacctgca tgagtccgga agaatacggg gcgttgcgca accgcctgtc gcagcgaaat
     4741 aactaatgta gccctgcgta ggccgtatct ccccgggggg ggacggttgc gatcgatgca
     4801 cgtgagcatg tcacgtgttc agtaggcaaa cggatacggc gcgcgtcgtg agcggacaaa
     4861 tcgtgtccga gggggcgaag catattaacg atttgacgtg aaaggccgga agtcctcgcg
     4921 taggatgacc tcgtagggaa tgggaggtcg tcagcatggt gcgcgcgaca aagggtgggc
     4981 ggccaagcgc ccgcggacag gtaacgttat cacctggatc aatggcgaag ttttcccgtg
     5041 aacggggcgg cgcaggcgcg cccgctgcgc gtgcggacaa cgcggctagg gctgcgcgtg
     5101 agaatcgtga gaataacgcg cagcgagata ccgagcgacc aacttggctc gagctgcggt
     5161 atcgggggag aactctaatg ttcgaggtca ccgagggcgc gcgtccgctg acgctcgggg
     5221 ctggcttgtg ttgtgatctg cgggtggacg gcgccggggt tgccctggtc cagttcgagt
     5281 tggcgcgtca atcaggtaag gtctggctgg tgccgaagcc cgcggcacac gtgcgagtga
     5341 acgcagctca cgtggggcaa ccgcgtgagc tgtccacgcg atctgtaatc gagtttctcg
     5401 agcacgaaat cgaggtactc ctgcacgcaa gattcccggg acaatctagc gcgggcttgc
     5461 ccagcgctgg tgcttcgagg gtcagcgccg agaacgcctt cgccagagag ggaacggcga
     5521 gcggtcctgc gcgagacgag ccggcgccgg ttacggtgag ggcattcggg aatctcgcag
     5581 ggttagtgga gcgttcgccc acttcctcgc gcggttcggt gctggaggaa gcttatgcgc
     5641 cggtcgaggt tgcggcccga accttgccga cgccggcttt cccgccccca cctacccagc
     5701 ttgcaccgga gtgcgggacg gagggcgggg ccggtacttg gcttgccatg cccgctcaac
     5761 ggcgcactct ggacgggcgc tgaagcgcca ggttctccac aacgggcctc gagctgaaat
     5821 ttcgcgcctc ctaggcgcag gccagtaatg cctcgcgatt taggcctctg ctcggctgct
     5881 gacccgctcg ccagagccct gtttagccac cgctgagtca ggtcttctcg ggcttttcgc
     5941 gaaagatcgc gtctgcttgc ggaagggcgc tatccttcgg gccatgcgcc ttgctcctag
     6001 tcacccttcg agcccctgga gtttggatgg gttgcttggg tactgcgtcg tttggatagt
     6061 cgctgccgcg tgctccaatt ccggggaagg tagcgggaac ttaggtggtg cagccgggag
     6121 tagcggcgcc tctagtgggg cgagcgcggg ggcctcacct tcgaacgcgg gcggagcgtc
     6181 gtcgggcgga acatctgcgc cgaatccagg gggaagctcg acgacgctcg gcggcacgac
     6241 gaactcgggc ggcgcaatac actcgggcgg cgcgatgcac tcgggcggtg cgatgcactc
     6301 gggcggcggt acctcgggcg cgctcggcgg catgacgcag tcgagcggcg gcaactcgtc
     6361 gagcacgaca acgactgagc gtccggactg cccaatgtat cgagacaaga cgcacagctg
     6421 cgaatgctat tgtgactgct tgcagcacga ttgcgcaggt gacgcgagca tcccgtcagg
     6481 aatgacttgc cagcagttct gctcgacgtt cactgccgag caactggctt gtcgagtcga
     6541 ccactgcaag cttcgaatcc cggagagcaa tccgggccac tgtctacacg ccgtcgggga
     6601 cggcgcatgt gaatgaactg ccaaagcgag gtcgagccag ggtctgtttc gggggctctg
     6661 gagcgggcgc tgagcgtgct ccggacggtc gtggacatcg tggaaacgcc agtggatggc
     6721 cccgagccgc cggagtggtg cgttcgaaga ggctgggcgc ctcgcttatt ggccttgagc
     6781 gaaagtgagc tcgcgagctg tgaggagcga gggctcggcg cggtcgttcc gcagctcgcg
     6841 agctgccctg cggagtggct cgagctcagc tcggtcttac gtgcgtgcac cagcttgccg
     6901 cgcttggcta gcgtcaaagc cgagctcccg agccgagcct tgcgtggcgt gagcgcgcgc
     6961 aagcgagagc aactggcaga gttcattccc aacgtcgcgg cgctcgttcg gcgctcggag
     7021 cgcgtcgtgg acgtgggtgc cggcgcaggc cacctttcgc gtttggtcgc agagttgttt
     7081 cagcgcgacg cctgggctct cgatcgggac gctcaacggc tcgaacgagg tagatcacgg
     7141 tgccaagatc gagcgcgcga agtaggcgag ttgaacgtac gcttcgtcga agctcacgtg
     7201 ggggagcacg aactcgagtt tgctcccaat gacttggcgc tcggcctgca tgcgtgcgga
     7261 gagctggggg atcgcttggt gcagcaagcc gcgcgggtgc gttgtcacgt cgggctcgtc
     7321 tcttgttgcc tgcagaagac gcaggcggag cggcgaatga gcatggctcg cgcggcgcgc
     7381 ggcttcacgc tgcgccgttc agcgctgggc ctcgccaact tgacggctcg cgcagagggc
     7441 gtcgaggcga ccttggcgga gaacctgcgc gcacgcgaag cccgcctcgc cctacgctac
     7501 ttgctatgcg agcgcggcat cgaactcaag ccgggggaag agatgcgcgg catcaatcgt
     7561 cgccgggccg tggccgggtt cgcggagctc tcggcgcatg ccctggccgt gctcggactg
     7621 cccgccgcga gccccgcgga gctgagggcc cacgtggagc gcgctcgccg tgactacgcg
     7681 gtgatgcgcc gcctctctct gccgcgcaat tggctctcgc gcctagtgga agtagcgatt
     7741 gtgctcgatc ggggggcgcg gctcgaagag gccggccttc acgtgcgggt cgcgcagctg
     7801 ttcgaacaac gggtcacccc ccgcaatacg gtgctcttcg cgagtcggga tgcacaactg
     7861 ctcccgccct tgagcggtga atcggcgcgc ggactggatg cgagcgacgc gacgccaaaa
     7921 cgaacattcc tggtggggac cgctaacatg cataatcggg atcaggatgc cagctcagga
     7981 taaccgcgcc gagcccaccg cgagtgtcga gcggcccacc gggcttggcc cgatcctgct
     8041 ggtggatacc tactctctag tgttccgtgc tcatcacgcg ctgcccgcaa tgaataccca
     8101 agcgggtgag ccgacctccg ctctgtatgg cttctcgacc gtgattttga agctcctgaa
     8161 agagcaacgg cctgcggctt tggcttttgc tttggatgcg cccgaacgca ctttccggca
     8221 cgaacagtac gcgccataca agggtacgcg tgagcgtgct ccttcggagc tggtcgcgca
     8281 attgcgcaag ctacccgagt tcctgcctgc gttcggcgtg ccggttcatt gcgctccggg
     8341 tttcgaggct gacgacgtga tcgcgacgct tgccagcgaa ttcgagcccc gaggtcggca
     8401 aacgctgatc gtgagtggag atcgcgattt gttgcagctc gtgacgccgc acacgcgcgt
     8461 gtggttcgtg ggtgcgcggg ggcagaagcc gaccttgttc gatcaggagc aagtgcttca
     8521 gcggtttggc gtggagccgg cgcaactgcc gatgcgcgcg gcgctggtag gggatacttc
     8581 ggataaccta ccgggggtgc caggagtggg cccgggcacg gcggccaagc tcgtgcgtga
     8641 gtttggtacc gtggaggccc tggtgcgtgg cttggggagc gtgaaaccca gtcgcgtgca
     8701 ggaggcgctg gcaagtcacg tgcaacaaat cgtgctcaat gaaaagctct gccgtctagt
     8761 tcgagacgtc ccgctctccg gcgctccgcc ggcggccgcg ctgtctgcgc agagctttgc
     8821 tcgacttcgg caggccttcg agtcgctcga gttccggagc ctggtgcctc ggctcgacgc
     8881 gatcgagcgc atcacgctgg agacacgagc agtctagaga gcagtttgga ggtgaggagc
     8941 tgttcgggat cgacctgcct cttcagttca cgcagacgag cgacatagtc ggcgtgcatc
     9001 tcgcgcagca agccgtcgtc gcaagtgctc tgcttgagca gcagcacttt gaagcgcggg
     9061 tttcgctcga aggctacccg cgaaagttca ctgtagaagg cggccgctcg cgtgtggcct
     9121 gcgctacccc tcctgaccgc gatgctgggc gtgatatagg cggatccttg ggtgttgccc
     9181 catgtgccgt gggccggcca acgacatacc ggtaatagga tcaagtcctg ttgttcagcg
     9241 cgacgctcca ctcccgcgta ccgctgcatg acttcccaat acacgctcat gaactccgcc
     9301 acgtgctcgc gcggaaaaca ccaagtttga tggcagacgg tcaggcgcgg gtcgaccccc
     9361 aacgcccgca gccccgcaaa cgtgagactg cgactggata agatatcgta cgcagtgtcg
     9421 tagctgcgct ggaagaagag aaagccgtac caatccgccg aaaaagcccg gcccgggcgg
     9481 aacgcgccga gcgcgatgtg tcgcgcgagc gccggatgac gattcgacag cccttgcagt
     9541 gccgcgtaga atcgagggcg atcgccggtc agcggtgcct ccgtcaaccg gcgcgacgca
     9601 cggttcgagg cgatcgaatc tgccaccaac acgtatcgat cccgcaatcc gtagaagaac
     9661 aatccgtgac ccggcgcatg cttcggatcg gccgcgatgc ggaacagctc ttcgtaacac
     9721 tggggctcgc gtactttgcc gcagctagcc acctcgatac acaaagcaaa atccggtggc
     9781 acacgctcca atcgaagctc gatgtccgtg aacacgccga gcgccccaaa tgtgccgggc
     9841 gcgaattgga acagcgcctg ctcgagggcg ttacctcgcg gagaacagcg gtgactccgc
     9901 ccatcggggg agagcaaagc aaaggcacgt acgccgtccg cgaagaagcc cgccatgttg
     9961 tgggtgcagc tcgccagcgc gccgctgatg ctgatcccgt cactcgtggg cgggctgcgc
    10021 ggctgatact ccgggtactg tgcgagtagc tgccggaaag taacaccagc cggaactttc
    10081 acccagattg ccccgtccgg ctcgcgctcc acgagcgtgg ccggctcacc cagatcgcga
    10141 acgtcgagcg cgtcggcacc tgggggacac aggaagtgcc ctccaaagga cttctttgcg
    10201 ccgaccaagg tcagcttccg gccgtggctg agcgcctgag cgtaggcctc gaatacctct
    10261 ccggtcgagc tcggaaacca acaccgggca gtggactcgt ccaaaccgcc tttggcgagt
    10321 agctcgacga cttcgctgtg catgcgccgc gcagcctaga gcaaaggctt gaaaaccaac
    10381 aagcgaaagc gggtcagcgc ttcatctgca tcagcatcga gagcatatcg tccgcggtag
    10441 cgatgacctt ggaattggcg ctgtacgcgc gctggtgcgt gatcatgccc acgaactcct
    10501 cgccgaggtc gacgttggag gcctcgagcg ctccagaact gagtgagccg cgtccaccac
    10561 taccgggcgc accgagcgcg ggatctccgc tctccgccgt tgcgacccag aggccttgtc
    10621 cggcccggcc gagcccttcc tgggagcgaa acttggcgac tacgagttgc ccgaccggca
    10681 agcgcaaccc gttggagtac aagccttcca ccacgccggt ggagccgatg gacactccgc
    10741 tgagagcccc ggaagcgtag ccatcctggc tctccgatga ggtgctgctc ggcatgctga
    10801 actgggtggt gccgtcgagg cccgtgccgc cgtccgcgat ggtcgaaccg aactcgagag
    10861 cgacggtctg gcccgcgatg gcacccccgc cgaagctgac gctgattgcc tggttggtgg
    10921 caacactgtc cagagcgccg ttggagttga acgtgaggct gcccgcgcca acctcagtgg
    10981 gtccggcgac gaccgggtcg agatccgagc cgggcaggaa agcgtggtag ttccactgcc
    11041 cgtcacctac cttttcgaag tagatatcga gtgagtgggc cgcgcccagc gagtcataaa
    11101 tttggatcga tgtcgaagcg ttggcggtgg cgctcggagc caagggatcg aaggtggccg
    11161 cgggcgccga ggcgtcgagg ttggccgcga gttggatatt ggccgttgcg cgtgccggaa
    11221 tgcccgcagt cggtacctgc aacgacgaaa cgccagccgc gagcgtgccg tccgatttgg
    11281 cgggccttcc aacgagcttc agtccttcgg ggttgacgag gtagccgctg gcgtcgattt
    11341 tgaattgacc cgagcgcgta taaaaggccc ccgttacacc gtttgtggcg ccggtgacga
    11401 cgaaaaagcc gtcgccgttg agtgcgagat cggtggcaat ccccgtttgc gtgagcgcgc
    11461 cttgcgtgaa ggcctgttga gttcggctgt tctctacgcc cgcgccggat ccgttcccgg
    11521 agccgctctt caagagcacg tcttcgaaga tcgaacgctg gcgcttgaaa ccggtggtgt
    11581 tgacgttggc gatgttgtcg cccaccacgc ccaaggcctc gccttcggca cggagtccgg
    11641 atactccatt ttcgagggcg cggagagtca cgcccctagt ctgagcaata tcggggccac
    11701 cacgcgggtg cgcgcgagag gcgcgtcagc ccgacatggc cccttggctg cgggcggggg
    11761 gagccatccg ccttaccagc cattctgggg cgtgcgccac gacatgcctc agcaggttca
    11821 tctgccaggg cagcgtgaag gttgccttgc gctgctcgac ggcttgaacc atgtcccgcg
    11881 cggcttcgtc gacttccatc aggaatggca gcttatgcgt cagggtcgcg gtgagagggg
    11941 tgcgaacgaa gccgggacac aaggtcatcg cgtgcactcc ggtacccacg agctgcatcc
    12001 ggagcgcctc catgaaggtg agaattgccg ccttcgaagc gctatacgca gctcgcccgg
    12061 gtaagccgcg gaaaccagcc accgagctga ccgctacgag ggtgcctgtc ccttgcttca
    12121 gcatcatcgg gatggcgggc acgagagtgt tcgtcacgcc aattacgttt actcggatca
    12181 actgcgcgac cgcctcggcg tccccgctac ggagagtgtc gccgatccca atacccgcgt
    12241 tggcaatgac gcggtcgatg ctgctggcgc ccccagctgc ggtggccgca aagtcgtcga
    12301 gtgcgacgcg catcgccgaa gcatcggtga catcggcggc atatacgtgc accttcgcgc
    12361 cggcttccct gagctcggac gcgagccgtt cgagcgcgtc cgcgcggcga gctaccaagc
    12421 cgagtcgagc cccgcctttg gcgtactcac gggccatggc ggcgccaatc ccgctgctag
    12481 ctcccgtgat gaacaccttc atgctttcct cctcgatcat ggccgcacgc taggtcaact
    12541 ggcggcgcgg gccaaatcac gaatggggcg gcctctccaa tgcctcgaaa gcgctggagc
    12601 gaggcgctga gctacagcct gggagcggaa tgcgccgtgt ggggcatttc gcgcttcaaa
    12661 cgagcgaatt tactggtcta ttcgtcgagc cgcgtcgtga gattgtgcaa cgcttactcg
    12721 atgcaagcca tccgttcgag accgaagttg gtagagcgct tcgcgccgcg tgcacctgct
    12781 ctgttcctcg tgatcctcgc gttccagttg aatggctgcg aagcggtgaa gggcatcttc
    12841 aaggcgggat tttgggtcgg agcgctgttc gtgattggca tcctcggttt ggttgcctac
    12901 ttgctcgggc gcaagcgctc ctagcgttca ggcacgtgga gcgctggcgt gccgatcgga
    12961 atcgagactc gacgccacgg cattcagcgt ttgctcgagc ttgggcaggt ctggaggctt
    13021 ggcgaagtgc gcctggaaac cggcctcgga tgagcgccga cgggcgtcgt cgctcgcgta
    13081 tccagacagc gcgatcaagt gtattcgaga caagctcggc tctcggcgga ggctacgcgc
    13141 caccgcgtac ccgtcgaatt cgcccggcaa tcccaggtcc gagatcacga catcgggtcg
    13201 gaattcccga gctagagcca cccccgcggc cccgtccaga gccagcttca cctggtgtcc
    13261 ctgcagcgtg agcagttctc ccaacgtctc ggcggcgtcg gcgttgtcct ctactaccaa
    13321 gatgcgcagg gggcggtctc gcgcggcggg cgtgctgctc gaaggctcca tgcgctggcg
    13381 cgactcggaa agcggtacgc gcagcacgaa cgtggagccc tggccgggac ccgggctgtg
    13441 cgcttccacg cgccctccat gcagttcgac gagcccgcga gtcaaagcca agcccaatcc
    13501 taacccaccc ttcgagcgag cgaagtcttg atcggcttga ctgaacgggt cgaagagacg
    13561 ggacaaaaag cttggctcca tgccggtgcc ggtgtccgct acgctcacga cggcttgtcg
    13621 cgagcccgta tcgcggctgg tgcggactcg cacgcgcccc ccgctagggg taaagcgact
    13681 cgcgttgtgc aataggttac cgaccatctg ggcaagccga accggatccc cttcgaccca
    13741 gagtggttcg gtgccttcgt cgatcgtgag cgtcaaaccg gcgacctcga gagttgcccg
    13801 gtagtcatcc gccgtctggt gcacgatccc gcgcaaatcg cagggctgct tctggagttc
    13861 cagcttgccg cgcgcgattc gggatacgtc cagcaagtcg tcgacgagac gcgtcatgtg
    13921 cgtgacttgc cgcgcgatca cgtctcgggc tcgcgagagc tgcggcccgt ccgtgccgct
    13981 acgtttcaag atctcgactg cattgcggac cggcgcgagc gggttgcgta gctcgtgagc
    14041 cagcatcgca atgaactgat ctttccgtct atcggcgtcc ctgagctccg cttcggcgcg
    14101 cttccgctcg gtgatgtcgc ggttggattc gagcacccaa acctggccat aagctcgaac
    14161 gagttgttgc cgactctcca caatcagctc gcggccatct cgggtggtgt gccgcagttc
    14221 accggtccat tccccactct gctccagctt ctgatagatg agctcgaggc gtat
//
LOCUS       DASTKU010000733        10007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_365263, whole genome shotgun sequence.
ACCESSION   DASTKU010000733 DASTKU010000000
VERSION     DASTKU010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10007
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365263"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1528
                     /locus_tag="VFQ61_25765"
     CDS             233..1528
                     /locus_tag="VFQ61_25765"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HET9957942.1"
                     /translation="MISQEEYRLRRERVLQEIHPGALLLFAAPVSIRNNDVEHEYRQD
                     SDFFYLTGFDEPQAVLLLRSTAQPSSVMFVQPRDPERETWDGPRAGVDGAVRDFGVEQ
                     AFPVSELDAKLPELLGGFERLFYRLGDGPRDERVLGAVQNIKRKTRGRTLWPNQILEP
                     DSILHEMRLHKSSSEVAVMRKALEITGRSHVELMNTTRPGMYEYELEGKLRRTFREHG
                     AERPAYSPIVGSGPNATILHHRRNDRRIQEKELVLVDAGCEYGYYASDVTRTFPASGT
                     FTSAQARLYEIVLDAQLAAIEKTGVGATIDSVHQAALEVIVDGLIRVGLIEGPREKAL
                     ESGSYKTFYMHRTSHYLGMDVHDVGRYYSSGEPRPLEPGVVITVEPGIYVPLAEQYGE
                     YSGIGIRIEDDVLVTEASAEVLSSEIPKTRAEVEQACAR"
     gene            1516..2316
                     /locus_tag="VFQ61_25770"
     CDS             1516..2316
                     /locus_tag="VFQ61_25770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957943.1"
                     /translation="MRALTAALSVVLWCSSAQAAEPTNASAKGKPGIKSRAELDAEAP
                     RTGTIERESRPTRNQENAADESVPSEPGGVESGGEPTNEAQLTSRDTASIADGVFARG
                     PYLHTFASVGYGRGMRFNNPFRLASPAGERADGLSLTAPYLDLSIAGLLGPPRGLQHG
                     VMLRTSIALTGVPQEVLSVGYVVARALGNAALGYARLSVPIVLRPDLNAGGELGLGAA
                     YWLRAGIGVSAELAGSLFAGAATWESDPTLIPVVSLAISACFEYEVLP"
     gene            2313..2726
                     /locus_tag="VFQ61_25775"
     CDS             2313..2726
                     /locus_tag="VFQ61_25775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957944.1"
                     /translation="MSALESSWFGASRWKKALRSGFTLLLLAFCAAPTPGDIGGCGSQ
                     AEPLSPEAFFAAKAVLDCARCDECGLSSRACTQACSAQPPTRDFAVACVPVVHDGDVC
                     LHALSAASCDEYAAFVRDRSPSVPNECNFCPKESP"
     gene            2723..3754
                     /locus_tag="VFQ61_25780"
     CDS             2723..3754
                     /locus_tag="VFQ61_25780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957945.1"
                     /translation="MKSKRSGHRIGLAIGILPLLWGCATTRRFSAPRADYAAYREVRT
                     AAHPEQKLHAADAYLRRYPDGAFHDEVQAWFSQAEPEFFRQAWDRPSLLRAYQRALPR
                     GPHEKQVSDRLIEFNLWNEYRARQESKAESFLSRVERDLQVAEESRRDFLQRITKLVG
                     LFSDSRAYGLPTTEQNHELIYEFRLRPPAGHCEPRACRKLFELTFAVPHEKRLQARSA
                     RLELRFELQNERVVAFTLAGPELFSRLAEASDRTYIAETDLLARTEGIARAVQVLSNA
                     LEPRLPAAECERAVVAPVVMERACRGVLLRARVGEGTDMVEGRAASDAELHPTLAPPS
                     KPAPPRKRP"
     gene            3751..4641
                     /locus_tag="VFQ61_25785"
     CDS             3751..4641
                     /locus_tag="VFQ61_25785"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HET9957946.1"
                     /translation="MNTPSIPYVELPVLPLWPGQEALSLKPFGLLVAIGVSLGAASAS
                     RYGKRRGLDAATLTSFIYWVVGSGFVFGHVLDVLLYSPERLKDDPLAIIRLWDGLSSY
                     GGFVGGVLGALAFRIQRGVPVLPYADVVASGLPLGWVFGRAGCAVVHDHPGILSQAWF
                     AVRFPGGGRLDLGLLEMALSIPLAVAFALLKKRAWPWGFFIAWMCAIYGPLRFALDFL
                     RVAEPTWIQGSLYEPDRRYLTLTPAQWASLLVFAFGLYMLLHVRSRVDRDSAFFPPKP
                     ATRAEVVRARRSSIEEPPAH"
     gene            complement(4670..5596)
                     /locus_tag="VFQ61_25790"
     CDS             complement(4670..5596)
                     /locus_tag="VFQ61_25790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET9957947.1"
                     /translation="MRVRVIVNPKAGSGSAARRLPEILREIERSGALSDSAETRAAGD
                     AARLVREAREEGIDCVAVLGGDGTFNEACQAYIDDQGRACAGPDLAVIPSGTGGDFRR
                     TFQLGTSTQEAVARLMSSAARPVDLGVIDAVAHDGTMRRHAFVNITSFGMGGLTDRIV
                     NRSPKWMGGRVAFFVAALRALAVYRNAPVRVTVDDEVCVEGPIVNVMIANGRYFGGGM
                     KIAPDADPSDGLFDIVVIGDVSRSKSLSFTPHIYRGTHVDQPGVLVRRGRVVRAEPLV
                     KDLEVLIDMDGETPGRLPLEARLLPCALRVRI"
     gene            5777..6670
                     /locus_tag="VFQ61_25795"
     CDS             5777..6670
                     /locus_tag="VFQ61_25795"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957948.1"
                     /translation="MATEDARKLFVAGLPESITEEVLRQLFEATGGKVVDVSLPRERA
                     TGKVRGFGFVTFATSEQATHARDELDGSLQAGRSISVRPFSSEPPKRGEVRSEAPAPA
                     APAEDRTLYVGNLPYDTSQQELSQFLNDYGVGPIVRIHLPVGPDGRMRGFGFVTLASG
                     DAAQAALGALRTAEVRGRRLMVNIAHPRAAGGAGPGAGPDRSRPPPRMDGGRPGEPRY
                     PTEPPMSFEPGGGAPLDDRRPRSGAPPEGGGGGEWSEGAAPKKKKRGAAKGASKERSA
                     DSRRRGGGGSNLVRGDWDDDD"
     gene            6968..8002
                     /locus_tag="VFQ61_25800"
     CDS             6968..8002
                     /locus_tag="VFQ61_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET9957949.1"
                     /translation="MIFDWLYGLFSNDLAIDLGTATTLIYVKGKGIVSCEPSVVAVSR
                     DARGEKRVLAVGREAKDMLGRTPGNIQAIRPLRDGVIADFEITEAMLRYFIARAHNRR
                     TLVKPRIIICVPFGITEVEKRAVKESAESAGAREVYLIEEPMAAAIGAGLPITEPSGN
                     MVVDIGGGTTEVAVISLAGIVYSQSVRVGGDKMDEAIVAYMKRKYNLAIGEQTAERIK
                     VSIGNAYPLEQQLTMEVKGRDMVAGVPKTVVVNSDEIREALAEPINAIVEAALLALER
                     TPPELAADIVDKGVVLTGGGALLKNIDVLLREETGLPVMVSDDPISAVVLGSGKTLDH
                     LELLKEVTIG"
     gene            8085..8960
                     /gene="mreC"
                     /locus_tag="VFQ61_25805"
     CDS             8085..8960
                     /gene="mreC"
                     /locus_tag="VFQ61_25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET9957950.1"
                     /translation="MTAFRRYRDIVVVVLLLAVPFFFLRASIRKPSELTPIDQAVLRI
                     AAPLEYVSSALARGVSSLFGEYVYLVDVKEDNSRLSYENARLRSRVRELEHAEAESHR
                     LRRLLGMRDTIADETVSAVVVGKDTTEYFRVAHVTIDTPNPRVRVNMPVIALDGAVGT
                     VERVAGEKVMVQLAVDSGFGVDVVVERTGARGFVRGMGERGRYAVRVEYVYRTDEVEI
                     GDLLVTSGVGCRFPKGIPVARVKKLQKREFGIYQSVEAEPTVDFSRLEEVLIILNDSE
                     DCDTATKARKARAQR"
     gene            8967..9512
                     /locus_tag="VFQ61_25810"
     CDS             8967..9512
                     /locus_tag="VFQ61_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957951.1"
                     /translation="MRGAAYIGVAVILLLIQANLFRLFGPLGAWIGARWVHGLSPDLV
                     LPLILFVGVHEPSMSKGALLAFGIGYAKDLLAGAPVGLFTFVSVAVWWVSRVAGVRLT
                     AQTWLTRVSLGAVFSVVEGALLLVLLAVFGSDNRRPVELASVVLPHAIFTGLCSPMLF
                     RLAQRLRQGPTPVRAAAEAAP"
     gene            9509..>10007
                     /locus_tag="VFQ61_25815"
     CDS             9509..>10007
                     /locus_tag="VFQ61_25815"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9957952.1"
                     /translation="MSFLVQRSDVGEFRRRYRWLVLFVLLLFSVLIGRLVQLQLIEGD
                     AHRAQARKNIVRERYLATTRGVIRDALGRVLAANRPSYTVYITPSRLDMQATWPKLVR
                     LVGLDESERQRLEKRIGEIKEAVAQRNPKERDRRADQQLVLKYDVDRDAVAWLETHEP
                     QGVDVT"
BASE COUNT         1765 a         3014 c         3352 g         1876 t
ORIGIN      
        1 cttgcgtccg cggggccacg ccctgcgtcc gcccggggct taactccgtt ccagcatccc
       61 cctcaggcgt agcaggggct cgcggtgcgc tctcccgcgg gtccaccggg actcggcggg
      121 ctcagcgagc gcactagtgc gctcgcgggt ggatgggggt cgagtccggg ccttttcggc
      181 cacgcgcaat ctcactccgc cgcgcccacg aggtaatggt agaagatccg ccgtgatttc
      241 tcaggaagag tatcgactac gtcgggagcg cgtgttgcag gagattcacc ctggggcgtt
      301 gctgctcttc gccgcgcctg tctcgatcag gaacaacgac gtcgagcacg agtaccgtca
      361 agattccgac ttcttctact taactggctt cgatgaacct caagcggtgc tgctcctgag
      421 aagcactgcc cagcctagca gcgtgatgtt cgtgcaaccg cgcgacccgg agcgcgagac
      481 ctgggatggg ccgcgcgcgg gagtcgacgg ggccgtgcgc gacttcggag tggaacaggc
      541 gttccccgtg tcggagctcg acgcgaagtt gcctgagctc ttgggcggct tcgaacggct
      601 cttttaccgg ctgggcgacg gtccgagaga cgagcgcgtg ttgggtgccg ttcaaaacat
      661 caagcgcaag acccgcggcc gcacgctttg gccaaaccag atcctagagc cggactcgat
      721 actgcacgag atgcgcttgc acaagagttc gagcgaggtc gccgtgatgc gaaaggcgct
      781 ggaaatcacg ggccggtcgc atgtggagct gatgaacacg acgcggccag gcatgtacga
      841 atacgagcta gagggcaaac tgcgacgcac tttccgcgag cacggtgcgg agcggccggc
      901 gtactccccg atcgtaggct ctgggccgaa cgccacgatt ctccatcacc gccgcaatga
      961 ccgacggatc caagagaaag agctagttct cgtcgatgcg ggatgcgaat acggatacta
     1021 cgcatccgat gtgacgcgga cctttccagc gagcggaacg ttcacttcgg ctcaagcgcg
     1081 tctctacgag atcgtactcg acgcgcagtt agccgcgatc gagaagacag gggtcggagc
     1141 gaccatcgac tcggtgcatc aggcggcgct cgaggtgatc gtcgacggac tgatccgggt
     1201 gggactgatc gagggacccc gcgagaaggc gctcgagagc ggatcttaca agacctttta
     1261 catgcatcgg accagtcact acttgggaat ggacgtgcat gacgtcggcc ggtactactc
     1321 gtcaggcgag ccgcgcccac tggaaccggg agtcgtgatc acggtagagc caggaatcta
     1381 cgtaccgctc gccgagcagt atggcgaata ctccggtatt ggtattcgca tcgaggacga
     1441 tgtcctggtc accgaagctt cggctgaggt gctgagttcg gagattccga agacgcgcgc
     1501 cgaggtcgag caggcgtgcg cgcgctgacg gcggcgctct cggtagtgtt gtggtgcagc
     1561 tcggcgcagg ccgcagaacc cacgaacgcg tctgcgaaag gcaagccggg gatcaaaagt
     1621 cgagccgaac tcgacgctga ggccccgcgc accggcacga tcgaacgtga gtcacgcccg
     1681 acgcgcaacc aggaaaatgc agcggacgaa tcggtgccca gcgagccagg cggggtcgag
     1741 tcgggcggtg aaccgacgaa cgaggcgcag ctgaccagtc gtgataccgc ctcgattgct
     1801 gacggcgtgt tcgcgcgggg gccgtacctt catacgttcg ctagcgttgg gtatggccgc
     1861 gggatgcgat tcaacaaccc gtttcgcttg gcatcgccag cgggtgaacg tgccgatggc
     1921 ctgtctttga cggcacccta tctggatctg tcgatcgcgg gtttgctggg cccaccgcgc
     1981 ggtctacagc atggcgtgat gttgaggacc tccatcgcgc tgacgggggt tccgcaggag
     2041 gtattgagcg tgggctatgt cgtcgcgcgc gctctgggga atgccgcact cgggtacgcg
     2101 cggctcagcg ttccgatcgt acttcggccc gatctgaacg cgggcggcga actcgggctc
     2161 ggggctgcgt actggcttcg ggctgggatc ggtgtgagcg ccgaattggc cggtagtctc
     2221 ttcgcaggtg ccgcgacgtg ggagagcgac ccgactctga ttccagtcgt ttcgctcgcg
     2281 atttctgctt gtttcgaata cgaggtgttg ccgtgagcgc gctcgagtcg agctggttcg
     2341 gggcttctcg ctggaaaaaa gcgcttcgtt ccgggttcac cttgctgctg ctcgcgttct
     2401 gtgcggcgcc cacgccggga gacattggcg gctgcggtag tcaggcggag ccactttctc
     2461 cagaggcgtt ctttgctgcg aaagcggtgt tggactgcgc tcgctgcgat gaatgcggct
     2521 tgagcagccg ggcctgcacc caagcctgct ccgcgcaacc gccgacacgg gactttgcgg
     2581 tggcctgcgt gccggtggtg cacgacggag acgtttgctt gcacgcgctc tcggcggcca
     2641 gctgcgatga gtacgcagcg ttcgttcgcg accgctcgcc gagcgtgccg aacgagtgca
     2701 acttttgccc caaggagtcc ccgtgaagtc caagcgaagc gggcaccgga tcggtttggc
     2761 gatagggata ctaccgctgc tctgggggtg cgcgactaca cggcgattct ccgcgccccg
     2821 cgcggactac gcggcgtatc gcgaggttcg aaccgccgcc caccccgagc agaagctgca
     2881 cgcagcggac gcttaccttc gccgttaccc cgacggcgcg tttcacgacg aagtacaggc
     2941 ctggttctcg caggccgaac ccgagttctt tcggcaagct tgggatcgac cgagtttact
     3001 gcgagcttac caacgcgctc tgccgcgcgg cccgcacgag aagcaagtca gcgatcgctt
     3061 gatcgagttc aacttgtgga acgagtatcg cgcgcgacaa gagagcaaag cggagtcgtt
     3121 tctcagccgg gtcgaacgcg acctgcaggt ggcggaagaa tcacgacgag acttcctcca
     3181 acgcatcacc aagctagtgg gcttgttcag cgactctcga gcgtacggct tgccgaccac
     3241 cgagcagaat cacgagctga tttacgagtt tcggctgcgg cccccggcgg gtcattgtga
     3301 acctcgggcg tgccgtaagc tcttcgagct caccttcgcg gtgccgcacg agaagcgcct
     3361 gcaggcgcgg agtgctcggc tcgagctgcg cttcgagctg caaaacgagc gcgtggtagc
     3421 tttcacgctg gccggccccg agctgttcag tcgcttggca gaggcgtcgg atcgcacgta
     3481 catcgccgag acggatcttc tggcgcggac cgagggcatt gcgcgagcgg tccaggtgtt
     3541 gagcaacgcg ttggagccgc gattgccggc tgctgagtgt gagcgagctg tcgtcgcgcc
     3601 cgtggtgatg gagcgcgcgt gtcgcggcgt gctgctgcgc gcgcgggtag gtgaaggcac
     3661 ggacatggtc gaaggccgcg ccgcctcgga tgccgagctg catccgacgc tagcgccccc
     3721 atccaagcca gcgccgccgc gtaaaagacc atgaatactc cttcgatccc ctatgtcgag
     3781 ctaccggtcc tcccgctctg gcctggtcag gaggcgctga gcctcaagcc gtttggactg
     3841 ctggtggcta tcggcgtgag tttgggggcc gcctccgcga gccgctacgg caaacgccgc
     3901 gggctcgacg ccgccaccct cacgtccttt atctattggg tcgtaggaag tgggttcgtg
     3961 tttgggcacg tgctcgacgt gctgctgtat tcgccggaac gtctcaaaga cgatcctttg
     4021 gcaatcattc gtctttggga tggcctatcg agttacgggg gattcgtggg tggcgtgctc
     4081 ggcgcgttgg cttttcggat ccaacgtggc gtgccagttc ttccttatgc cgatgtcgta
     4141 gcgagcgggc tacctttggg atgggtgttc gggcgcgccg gttgcgcggt ggtccacgac
     4201 cacccgggca tcttgagcca ggcctggttt gcggtgcgat tcccgggcgg aggtcggttg
     4261 gatctcggtc ttttggaaat ggctttgagc attccgctgg ccgtcgcgtt cgctcttctc
     4321 aagaagcgcg cttggccgtg gggcttcttc atcgcttgga tgtgcgcgat ctacggtccg
     4381 ctgcgattcg cgctagattt tctgcgcgtc gcggagccga cgtggattca gggctctctc
     4441 tacgagccag atcggcgcta cctgacgctg acaccggccc agtgggcgag cctgctcgtg
     4501 tttgctttcg ggctctacat gctgctgcac gttcggagtc gggtggaccg cgactccgca
     4561 ttctttccgc ccaaacccgc tacgcgggca gaggtggtgc gggcgcgccg cagttcaatc
     4621 gaggagccgc ccgcgcattg agctgcgcgc atctccgcat gcgtttccgc taaatgcgaa
     4681 cgcgcagtgc gcacggcaga aggcgagctt cgagtggcag acgacccgga gtctcgccat
     4741 ccatgtcgat cagcacctcc aaatctttga ccaaaggttc ggcgcgaacg actcgtcctc
     4801 gacgaaccaa cactcccggc tgatcgacgt gagtgcctcg atagatatgc ggtgtgaagc
     4861 tcaggctctt gctgcgcgat acatccccga tcaccacgat gtcgaacagg ccgtcgctgg
     4921 gatctgcgtc gggcgcgatc ttcatcccgc caccgaagta acgtccgttg gcgatcatca
     4981 cgttcacgat cgggccttcc acgcacacct cgtcgtccac cgtgacgcgc accggcgcgt
     5041 tgcggtagac ggccaacgcg cgcagggcag caacgaagaa tgccacgcgc ccgcccatcc
     5101 acttcgggct gcgattgacg atccgatcgg tcaagccacc catcccgaag ctcgtgatgt
     5161 tcacgaaagc gtgccgccgc attgtcccat cgtgggccac cgcatcgatc acaccgagat
     5221 cgaccgggcg cgcggcgctg ctcatcagcc gcgcgacggc ctcttgcgtg gaagtgccaa
     5281 gttgaaaggt acgacggaag tctccgcccg tgcccgaagg aatcacggcg agatcggggc
     5341 ccgcgcacgc gcgaccttgg tcatcgatgt aggcttggca ggcctcgttg aacgttccgt
     5401 cgccgcccag caccgcgacg cagtcgattc cctcttcgcg ggcttcgcgc actagacgcg
     5461 ctgcgtcgcc cgcggcgcgg gtctctgcac tatccgacag agcaccggaa cgttcgattt
     5521 cccgcagtat ttccggcaat cgacgagcgg ccgacccgct gcccgccttt ggattgacga
     5581 tgacacgaac ccgcatgggg cgggagactc acaaaaggcc ggccgctccg caatcaagaa
     5641 acgttcgaga ccccccaagt aatcgtggca ggggacttga acgcagaaag actgaacgat
     5701 atcgtcgaga agaattcccc gagccggtca atttgaccgg ccgtgttacg ttcaggcgac
     5761 gatcaaggtt aagaagatgg cgaccgaaga cgcacgcaag ctcttcgttg caggcctgcc
     5821 agagtcaatc accgaagaag ttttgcgcca gctctttgaa gcgactggtg gcaaagtggt
     5881 tgacgtaagc ctgccgagag agcgtgctac gggcaaggtt cgaggttttg gcttcgttac
     5941 atttgcaaca tccgagcaag caacgcacgc acgcgacgag ctagatggat ccctgcaagc
     6001 gggacgctct atctctgtgc gcccgttctc atccgagccg ccgaaacgcg gtgaggtccg
     6061 atcggaggca cctgcacctg cggctccggc cgaagacagg acactctacg tcggcaacct
     6121 gccgtacgac accagtcagc aagagctctc acagttcttg aacgactatg gcgtagggcc
     6181 gatcgtgagg atccaccttc cggtggggcc tgacggtcgc atgcgtggct ttggtttcgt
     6241 cactctcgcg agtggcgatg cggcgcaagc ggccttggga gctctgcgaa ctgcagaggt
     6301 ccgcggccga cgcttgatgg tgaacattgc tcatcctcga gctgccggtg gagccgggcc
     6361 aggtgcgggt ccggatcgct cacgtccgcc gccccggatg gacggtgggc gtccgggcga
     6421 gcctcgctat ccaacggagc cgccgatgtc cttcgaaccc ggtggtggag cgcctctgga
     6481 cgatcgacgt cctcgcagtg gtgcgccacc cgaaggcgga ggaggcgggg agtggtccga
     6541 aggggccgcg cccaagaaga agaagcgcgg cgccgcgaag ggtgccagta aagaacgcag
     6601 cgcggactcg cgccgccgcg gtggcggcgg cagcaaccta gtacgcggcg actgggacga
     6661 cgacgactga acatcaactg aacgtcaacc gggcgaagcg tgatgccgat cactcatttc
     6721 gggatcatca cgcttttcct caaatttggc ttcagtcggt tcaccgtcgc atgaacgatc
     6781 tttcagtgcg acgccgtgaa ttggagaagc cggtcggctg gaaagttttg tgggtgtcag
     6841 tggtgattgc acccgcttga ccctgcgtgc taggaccttg cccacggcga gccggcctct
     6901 ctgcaggaaa acggttttct gcgcagagga ccacgcaccc accacgaaag acttctgagc
     6961 acggtcgatg atcttcgatt ggctatacgg cctgttctcg aacgacctgg ccatcgatct
     7021 cggtacggcc accacgctca tctacgtgaa gggcaaaggg atcgtgagct gcgaaccttc
     7081 agtggtcgca gtgtcgcgag acgcccgcgg tgagaagcgt gtactggccg tcgggcgtga
     7141 ggcgaaggac atgctgggcc gcacgccggg aaacatccag gcgattcggc ccctgcgcga
     7201 cggtgtaatc gcagacttcg agatcaccga agcgatgctg cgctacttca ttgcgcgggc
     7261 gcacaaccgc cgcacgctgg tgaagcctcg aatcatcatt tgtgtcccct tcgggatcac
     7321 cgaggtggag aagcgagcgg tgaaggagag cgcagagagc gcgggcgcgc gcgaggtcta
     7381 cttgatcgag gagcccatgg cggccgcgat cggggcgggc ctaccaatca ccgaacccag
     7441 cgggaacatg gtggtggata tcggcggcgg taccacggaa gtcgccgtga tctcgctcgc
     7501 aggcatcgtg tactcccaga gcgtgcgcgt cggcggggac aagatggatg aagccatcgt
     7561 cgcgtacatg aagcggaagt acaacctggc aatcggtgag caaacggccg agcgcatcaa
     7621 ggtgagtatc gggaatgctt acccgctcga gcagcagctc accatggaag tgaaggggcg
     7681 ggacatggtc gcgggtgtgc ccaagaccgt ggtcgtcaac tcggacgaga tccgagaggc
     7741 acttgccgag ccgatcaacg ccatcgtcga ggcggcgcta ctcgcgctcg agcgtactcc
     7801 gccggagctc gccgcggaca tcgtcgacaa gggcgtggtg ctcaccggtg gcggagccct
     7861 gctcaagaac atcgacgtcc ttctgcgcga ggaaacgggt ctacccgtga tggtgagcga
     7921 tgatccgatc agcgcggtcg tgctcggcag cggcaagacg ttggatcatc tggagctcct
     7981 caaagaggtc acgatcgggt gatgcgggcc gggtcgacgt tgcggagaac cggccttgag
     8041 cttgggttgg cgtcgtttcc gatgggctcc tgtggagccg gatcgtgacc gcgttccggc
     8101 gctaccgcga catcgtggtc gtggttctat tgctcgcggt gccgttcttc tttttgagag
     8161 cgagtattcg caaaccgagc gagctcactc cgattgatca agccgtgctg cgcatcgcgg
     8221 cgccgctcga gtatgtgtct tccgcgctgg cgcggggagt ctcctcgctg ttcggtgagt
     8281 acgtctactt ggtagacgtc aaagaggaca atagccggct ttcgtacgaa aatgctcgct
     8341 tgcggtcgcg cgtgcgggag ctggaacatg cggaggccga gagccatcgc ttgcgtcgct
     8401 tgctcgggat gcgcgacacc atcgcggacg agaccgtcag cgccgtggtg gtcggtaaag
     8461 acacgacgga gtacttccgg gtcgcgcatg tcacgatcga tacgccaaac ccgcgcgtgc
     8521 gcgtcaacat gccggtgatc gcgctggacg gcgcagttgg cacggtggaa cgcgtggcgg
     8581 gtgagaaggt gatggtccaa ctcgccgtcg acagcgggtt cggggtcgac gtcgtcgtgg
     8641 aacgcacggg cgcgcgtggc ttcgtgcgcg gcatgggcga gcggggccgt tacgccgtgc
     8701 gcgtcgagta cgtgtacaga accgacgaag tcgagatcgg ggacttgcta gtcacgagcg
     8761 gcgtgggctg ccgtttcccc aagggcatcc cggtagcgcg ggtcaagaaa ctgcaaaagc
     8821 gcgagttcgg catttaccag agtgtcgagg ccgaaccgac cgtggatttt tcccggctcg
     8881 aagaagtgct gatcattctg aacgactccg aggattgcga cactgccacc aaggcgcgta
     8941 aggcgcgcgc gcagcgctga ggcagcatgc gaggcgcagc ttacatcggc gtcgccgtca
     9001 ttctgctcct cattcaagcc aatctgtttc ggctatttgg gcctctcgga gcttggatcg
     9061 gcgcccgctg ggtgcacggg ctttctcccg atttggtgct gcccttgatt ctgttcgtgg
     9121 gcgtgcatga gccctcgatg tcgaagggcg cgctgctcgc attcgggatc ggatacgcga
     9181 aggatttgct ggcgggtgcg cccgttgggc tgttcacctt cgtgtccgtc gcggtgtggt
     9241 gggtctcgcg cgtggctggg gttcggctca ccgcgcagac gtggctcacg cgggtgagct
     9301 tgggcgcggt gttcagcgtg gtggaggggg cgctgctgct ggtgctgctg gccgtgttcg
     9361 gcagcgataa ccgacgcccc gtcgagctcg ccagcgtagt cttgccccac gcgatcttca
     9421 cggggttgtg ctcgccgatg ttgttccggc tggcgcagcg cctacgccaa ggtccgacgc
     9481 cagttcgcgc ggcggcggag gcggcgccat gagcttcttg gtgcagcgct cggacgtcgg
     9541 cgagttccga cgccgctacc gctggctcgt gctgttcgtg ttgctgttgt tcagcgtgct
     9601 gattgggcgc ctggtgcagc tgcaattgat cgaaggcgac gcgcaccgcg cccaagctcg
     9661 caagaacatc gtgcgcgagc ggtatctcgc caccacgcgc ggcgtgatcc gggatgcgct
     9721 cggccgcgtg ctcgccgcca atcgtccttc gtacacggtt tacatcacgc cgagccgtct
     9781 ggatatgcag gcgacgtggc ccaagctcgt gcgtctggtc gggctagacg agagcgagcg
     9841 gcagcgcctc gagaagcgga ttggtgaaat caaagaggcg gtcgctcagc gcaacccaaa
     9901 agaacgggac cgccgcgccg atcaacagct cgtattgaag tacgacgtcg atcgggacgc
     9961 ggtcgcatgg ctcgagactc acgagccgca aggcgtggat gtaacac
//
LOCUS       DASTKU010000734         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1034917, whole genome shotgun sequence.
ACCESSION   DASTKU010000734 DASTKU010000000
VERSION     DASTKU010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034917"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VFQ61_25820"
     CDS             complement(<1..1223)
                     /locus_tag="VFQ61_25820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9957953.1"
                     /translation="MKSSSMPPPAADVVPRLALRDPDVWLKAVAWVVIIACALLILTF
                     SFGRDQGIYAVVGDGLLHGRVPYKDLWDFKPPGIFYVYALAQALFGKNMMAPRLLEVL
                     SLFGSVAAMRRLSSRVFEDRTVGVLGGAIMALIHAEMDFWHTGQPETFGAFFTLLGLM
                     LTLEESERRHLVPFAVGLCFGCAALLKPPLGGGALVCAAYLSQRARSSREGWLPMLSP
                     VGWVALGGALPALLCVAWFAARGAYHDFYWTLGEFTPGYTKLSWEGRRASDMLYYAIE
                     EAFFKFSALAAAGVVSAIAIQPVGRREREGLFLLLGVITIHVAGIAMQGKFFPYHYGA
                     TLPLISLLAGLGFHKLWKRCLFGGMGGPLAFFSFVAVCVPMRYAVRDLPQFFWERAQI
                     RFSYLLRIPPFDSREA"
     gene            1476..1549
                     /locus_tag="VFQ61_25825"
     tRNA            1476..1549
                     /locus_tag="VFQ61_25825"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1510..1512,aa:Arg,seq:tcg)
BASE COUNT          463 a          765 c          655 g          315 t
ORIGIN      
        1 gcctcacgcg agtcgaacgg agggatgcgc aggagatacg agaagcgaat ttgcgcgcgc
       61 tcccaaaaga actgcggcaa gtcgcgcacg gcgtaccgca tcggcacgca caccgcgacg
      121 aacgagaaaa aagcgagcgg cccgcccatg cccccgaaga gacaacgctt ccaaagcttg
      181 tgaaatccga gccccgcgag cagcgaaatc agcgggaggg tcgcgccgta gtggtacgga
      241 aagaacttcc cctgcatggc gatgcccgcg acgtgaatcg tgatcacgcc gagcaacaag
      301 aacaaccctt cgcgttcgcg cctgcccacc ggttgaatcg caatcgccga gacgacgcca
      361 gccgcagcca aggccgagaa cttgaagaag gcttcttcaa tcgcgtaata gagcatgtcg
      421 gacgcgcggc gaccctccca gctgagcttc gtgtagccag gcgtgaactc gccgagagtc
      481 caatagaagt cgtggtacgc gccgcgcgcg gcaaaccagg ccacgcacaa gagcgccggc
      541 aacgccccgc cgagcgcgac ccaaccaacc gggctcaaca tcggcagcca cccctcgcga
      601 gagctgcgcg cccgctgact gagatacgct gcgcacacca gcgcgccgcc cccgagcggt
      661 ggcttcaaca atgccgcgca gccaaagcac agcccaaccg caaacggaac caggtggcga
      721 cgctcgctct cctcgagcgt cagcatcaga ccgagcagcg tgaagaatgc cccgaacgtc
      781 tccggctgac ccgtgtgcca gaagtccatc tcggcgtgga tcagcgccat gatggcgccg
      841 ccaagcacac ccaccgtccg gtcctcgaac acgcggctcg acagccgccg catcgccgcg
      901 accgaaccga acaacgacag cacctcgagc aggcgcggcg ccatcatgtt cttgccgaac
      961 agcgcttgag ccaacgcgta gacgtaaaag atccccggcg gcttgaagtc ccaaaggtcc
     1021 ttgtacggca cgcgaccgtg caagagcccg tcgccgacca ccgcgtaaat cccctgatcg
     1081 cgcccgaacg agaacgtcag gatcaggagc gcgcaggcga tgatgacgac ccaagccacc
     1141 gcctttagcc agacgtcagg atcgcgcagg gcgagccgcg gcacgacatc cgcggccggc
     1201 gggggcatgg aggaactctt catcgtcgcg cgcccaagct agcgaacgca ggacaagcga
     1261 ggcaacccgc ctcatcagcc cacaaaaatc tccctgttcg gcaacgcttc cccgggccca
     1321 ctagtcaggc cgagggcggc agcacgccgg gtcaagccgt ccggtcgagc gctcgaaacc
     1381 accctgccct cccggccgga atcggaggcg agcctgtgag cttgacggcg cgcccattcg
     1441 cagggaaagt ccggacgccc gccagcaatg gcgatgcgcc cctagctcag ctggatagag
     1501 catcggactt cgaatccgag ggtcgtaggt tcgaacccta cggggcgcgc ctcacgttca
     1561 gcatcaacaa tgctcgattg ttgaccacgt gctgacgtcg cgtttttgct ctggcccatg
     1621 cgctggccca cctctggggc cgcgagcgct gccgtgggtg ttgcaccctg ggccccagaa
     1681 atccgctgcg cgagctcggg caccgcaacg tccaaccgaa cgagtggcgc gaagccgagc
     1741 tctgtgcctg ccgcgccttg ccgccggtac ttgttgatca tctgactcga cttgtggccg
     1801 gtgcggtcgg caacccacgc ctcggtgcgc ccgtttgcca gcgcaagcgt gacgaacggt
     1861 gcccgtagat cgtggatgcg aatcgggcgc cgcgagtcgg tcttggcgga gagctcgggg
     1921 cgcttgatcc ccgctgtctc gagatgccaa ggcttcccct gccaccagca cgggttcaac
     1981 cggcctactc tctcggaacg tgaccccggc attcggtgtc ggtccgtcgc tcaaaaatat
     2041 aagccaacat ccgctcgcgg atgcgccaag accctaatta tcgagaagga gccttctcca
     2101 atttccacgg agctcggtgt catgtcttca accacatgtt gattcgtgac tacgacgcgg
     2161 gcatggcacg agaacccgga tccgcatcgc tccacacc
//
LOCUS       DASTKU010000735        13068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_589286, whole genome shotgun sequence.
ACCESSION   DASTKU010000735 DASTKU010000000
VERSION     DASTKU010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13068
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589286"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            454..2316
                     /locus_tag="VFQ61_25830"
     CDS             454..2316
                     /locus_tag="VFQ61_25830"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9957954.1"
                     /translation="MRLSERLLQRRPGSASGCFSALLLLACTGEVEPLPGDMNASGSA
                     GRPAHPEAGGQSVEPGGGTSSSKPSGSSGGSSGAATGGAPLTGGAAGANTSGANTAVC
                     EPGEISAAPAKLRRLTPEEYANTARDLVGEKAKPELEQPVGDVIGALEVEKLGQAAHD
                     LAVLGGHHEFVPCAIDGKADAACARSFITKFGARAFRRPLSSEEQSWLEGVYQKLGEQ
                     PEADPAFTFRERIDALAEVILQAPQHVYVEEVGVADAALPDGVRRLTGYERATRLSYL
                     IWGSTPDEELTKAAASGALDTASGVRAAAERLLANPRAKQMARRFASRWLGLDAETKH
                     PALEDLPKSAERFPFDGTKLRAALRTETEALYERAFFAEGDSFRALLTSRDAYVNADL
                     ASLYGVAFPGGESEFAWVELPERERAGLFTRAAFLSAYAGAEYQSPIRRGVFLLRKVL
                     CQTLPDPPPDADNTPPAPSRGDQARTIRELTEIKTSGGSCTSCHSVINPFGFAFEHYG
                     AIGQYQAEETGTFQGKPYELPVNASTEVRGGDVHGAVTDALELSRLLADSAQAHDCAV
                     DGWFERALSRRAMSDDACWLSAVKEQFRASSNLKELVLALASSDAALHIREATP"
     gene            2313..3770
                     /locus_tag="VFQ61_25835"
     CDS             2313..3770
                     /locus_tag="VFQ61_25835"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9957955.1"
                     /translation="MKIQRRTLLRGLGGLAFGLPWLESLNSTAHAQAAGGPKRFIVFF
                     EHGGTISANDKDGDKYDGNGANNGVDGWAPIGGEKLELGAIHQPLQAHLDSLLVLRGI
                     NNRACQRQSPYNGDHGWANVTALTSANATQLGDKDEDIVSEGPSIDAVLAERLAERNP
                     VAFPSVNLTVPAHNYGTPFFRAARQPVEGEYNPMRAFDKLFAGVTEGAQPDRAALRAR
                     ALKASVLDGVGEGLSLFRGRLSAADRIAVDAHLEHIRGLERQLESIAPAVGCQKPTLS
                     GLDKNWDYYSVDIQRSGPAQIDIMLAAMRCGLTNVATLNIGDFYAKFLNPTFPAAYDI
                     GHSLHHSARDVGKAGPDGAKFQAWYDTMLANRRWRMGLLARLLDGLANTREGDGTMLD
                     NTLILCASEFSCGADHSVADLPILLAGKAGGRLRTGRHVNFNSKASADPQTRSYETRA
                     SLHNLFTSVLQLFDFPDAHFGSDHAWERGPLAGLS"
     gene            4017..4595
                     /locus_tag="VFQ61_25840"
     CDS             4017..4595
                     /locus_tag="VFQ61_25840"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9957956.1"
                     /translation="MSARSVLTRALDGEGAAIEELIAELTPPLLRAVRAMLGPEHPDV
                     EDLVQDTLIDLVDALPSFRGECTLLHFAIRIAARKASSSRKRARAGRGFVDRFWRREA
                     PLQAAVVNPREASLVERRRQLLDSLLAELPQAQAETMLLRSVLGYSLEEVAAMTRVPL
                     NTVRSRMRLAKETLRRRIETDEQVRELLGGEL"
     gene            4592..5524
                     /locus_tag="VFQ61_25845"
     CDS             4592..5524
                     /locus_tag="VFQ61_25845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957957.1"
                     /translation="MTARESHPEGLIDRARRGAATEPELCALAEHLALCPSCRFESAL
                     RSDADRAGLPRVSDELLIARLRRSTTRALVARGARPRRAHRARAVWLVAAALCVGSAS
                     LAGVLGYRVVSRASSAPPMASPGAVEKNPPRFAGRSALPSSSSLPAPADVEVVAPVEA
                     AVPVGSIPSTASSGPPPAKRSAAELFAEANRARRAGDIAQAARGYRELTQRFPASSEA
                     AVARVSFGRLLLDRLGNAKGALAEFEGYLNNAGHRALREEALIGRAVALGRLGRVKDE
                     RTAWQTLLRSFPSSTYATRAGARLGELDARAAAP"
     gene            5551..6693
                     /locus_tag="VFQ61_25850"
     CDS             5551..6693
                     /locus_tag="VFQ61_25850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957958.1"
                     /translation="MVILFGVCGLLWVAPSRGAERTVRVTIAAPPAEASGLEAVFAEL
                     LARLHVSIQATNERVLDAAAIAAPKKPTPELLAHVWVDLTRRGAATLYLLDVATDRLL
                     VRKIRRDPGGGELVQEELGHILQTSVEGLLAGELVGVPRHEILPLLEPPAQPSTRTEN
                     ESPASATGSVASPTRAESSLRRDRRASLADAPASRGDGPASRMLGGHAVLLYGAALFG
                     PDVPIVHGPTLGAFAHTGGKLELGLWLSLQLRLPVEASNGPVSARFSQLSPRALFGLR
                     LVGDAERALRFGIGGGVDLVRVETHSQPEADVRVVDPATRAFAIGRAGLLFDWRFSGA
                     LRFVGELDVDIDSSATRYVYLSRTGERVLLEPYVLRPALALGVATP"
     gene            6690..7943
                     /locus_tag="VFQ61_25855"
     CDS             6690..7943
                     /locus_tag="VFQ61_25855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957959.1"
                     /translation="MMLSTRLLTASVAIVALACSSGTQTLAWEGEGQGSAGSPGIVDP
                     AFPEDLLEPWHGGPSYYGKWPRGLWTDTAHFPIGVWMQNPDNAQRFRDVGINLFLGLW
                     EGPTDEQLAGLLEQGVRTICDQKGVWQSHLEDSTIAAWMSEWGPDNAQERAGEGYDPC
                     IPPSDVTATIREIAARDATRPVLLGFGRGLADPEWVGRGECTGRTDMYPEYAREADVV
                     GFHSYPVNSSQQLLLVAQGVDNLREYTDHRKPVFALLEAASIDGKVRPTPEQIRAMVW
                     MVLIHGAAGIHYYCHHYADPFNETDCLDDAPTASTIASLNRLIGQLAPVLNRPTVTNG
                     VMVRLVNSGNRVDVMLKRQADATYLFAVEMLGRATSVTFELQRFPAAAQAQVLESERS
                     LDVTNGVFIDQLPAYGVRVYRVVPR"
     gene            8309..9295
                     /locus_tag="VFQ61_25860"
     CDS             8309..9295
                     /locus_tag="VFQ61_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957960.1"
                     /translation="MTTAAVSGSLAERYPEDNGLASDPAVLFYDDFEAGWGRWDAPKQ
                     DTQHLFVESDAARAHAGSRFLRSTVTEAQLAEQEYISAAPRADFTRRVPTVYWRFYAQ
                     FKGASATPHHWVRMAAGTPEYASSGLANTVPPGDRGFWFDFDADTGDHFNFYVYWFQM
                     RSGRCNDGSVTPGCAGDQGTTYHYGNTFAPPGQDEFPRDRWFCVEIMAKANQVGQSDG
                     ELAFWVNDQLIGDYRPGFPNGTWLRDKFHTDGCTFSACTEPKPFEGFEFRSSDDVLFK
                     SFFMDAYYERATMERRRAELEARGLTVPSEQTILYDDIVVATSRVGCRRTRE"
     gene            complement(9415..11640)
                     /locus_tag="VFQ61_25865"
     CDS             complement(9415..11640)
                     /locus_tag="VFQ61_25865"
                     /inference="COORDINATES: protein motif:HMM:NF021945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family metalloprotease"
                     /protein_id="HET9957961.1"
                     /translation="MTARDLCFATALMTLTSCGSSSSAGGSGGRETTGGRETTGGHET
                     TGGRETTGGRETGTTAGSGGLGHSSGGATSGVPNGGNGTGGVAHASGGTAGSTSTGGN
                     AAGAEGGSPGGATVRDPLPRSYGSELYSFPEPNDAAACVERANQTAKIERVFFAQTHA
                     LEPGHPFFYLTADRPALVEVLVSGDGAAPDVRVSGTVSKPGSTTVEKLGPLCLAGPAS
                     LAATVDATKHTKADRFTATLPSTWLRPGLSLTVTAGASSKTFSSEQLAVAATPDVNLV
                     MLPMDVLNYNDEHSDKPPPASWLGDFASATPAARVRFGKIPGRAVLPKIATSHYTGSE
                     EGAPPVVLDRRLCDDDEAAGATCMSSDVEPMELLGTTINIIEAIHAANGDLAVTFYYG
                     NSEGFFPGGWGGGKNFVGADFGDVFIHEQGHAFGLQHWGEGPFQGKDSQYLYPYGGNT
                     DNGGGRGETWNYYQNIGEFTSPICQDEKNTKVYGKERSDAMQRTHSCEEWRSTGKGPW
                     DGYGDFSAFAILHNLRGGPDLTGKVSYRGASVPYHIGETKGYPNLVMDASGVRAVQRV
                     SGTVTDEESLNILFAQEWDVPVATIFGSYHPSVAAANILYPPIEFSGSLPKIVDPTDP
                     TTFAALSKDPYRKYFYWPKDLTVKVTYADDSEQVALVAQQGPPRDWTLGSGPWRRDMT
                     YFAVNVRGDKKVSKVELYHRPFVVRGAGDAMDGNIANQSLGITASNYMDGATLLTTWT
                     R"
BASE COUNT         2278 a         4191 c         4281 g         2318 t
ORIGIN      
        1 tgagcagctg gggctgctcg atgcgctcgg gctcggtgct agtccggcgt gtcagtacgc
       61 ggacggttgg ttctcgtaga gcctaagtgt gatggacgca gacggaaggg cgtccactta
      121 ctcgggtccg agttgccccg caacgggtcc gcggagcgag acagttccga aggagttctt
      181 cgatcagttt gcccaagaca acgcggaacc gtgtcccgac tgatatcaag agtcgatccc
      241 agatatttcg acgggctctt gattcggcgg gcagggcggt gtctgcaggc tcgatgttgc
      301 aaccaccacg gctgcgccat gtagaattca acggccgcac cgcacggaag gagggcaatg
      361 taggcgaata ctaaatcaat cgagcgcacg cattggggtg atcagttttt cgagttcgtt
      421 ctcactagag cgccgaaacc gaggacgaac gaaatgcggc tatctgaacg gttgttgcaa
      481 cgacgccctg gcagcgcgag cgggtgcttt tcagctctgc tcctgctcgc gtgtaccgga
      541 gaagtggagc cgcttcccgg cgacatgaat gcatctggct ctgcggggcg accagcacat
      601 ccagaagcag gcggacagtc cgtcgagcca gggggcggaa cctcgagctc caagccatct
      661 ggatcgagcg gtggctctag cggcgcggcc acgggtggcg cacccctgac gggcggtgcg
      721 gctggcgcga acacgtctgg cgcgaacacg gctgtgtgcg agcccggcga gatcagcgcc
      781 gcacccgcga agctgcggcg tcttaccccg gaggaatatg cgaatacggc gcgagaccta
      841 gtgggagaga aggcgaagcc cgagctcgag cagccggtgg gtgacgtgat cggagcactc
      901 gaggtggaga agctgggtca ggctgctcac gaccttgcgg tgctcggcgg ccatcatgag
      961 ttcgtaccgt gcgcgatcga cggcaaagca gacgccgcat gtgcgcggtc gttcatcacc
     1021 aagttcgggg cgcgagcgtt tcggcggccg ctctcgtccg aggagcagag ctggctcgaa
     1081 ggggtatacc aaaagctcgg ggagcagccg gaggcggatc cggcattcac gttccgcgag
     1141 cgtatcgacg cgctcgccga ggtgattctg caagcaccgc agcacgtcta tgtcgaggag
     1201 gtcggtgtcg cggacgccgc tctgccggac ggcgttcgtc ggctcacggg ctacgagcgc
     1261 gccacgcggc tctcctatct gatttggggg agcacgcccg atgaggagtt gacgaaggcg
     1321 gccgcgagcg gtgcactcga cacggcgagc ggagtgcggg cggccgcgga gcgtttgctc
     1381 gcgaatcctc gcgccaagca gatggcgcgg cgcttcgcgt cgcgctggct cgggctcgat
     1441 gccgagacga agcaccccgc gctggaagat ctgccgaaaa gcgccgagcg ctttccgttc
     1501 gacggcacca aactccgcgc ggcgctgcgg accgagaccg aagcgctgta cgagcgcgcg
     1561 ttctttgcgg agggggactc gttccgtgcg ctcttgacct cgcgcgacgc ctacgtcaac
     1621 gcggacctcg cctcgctcta cggggtggcc ttcccgggag gagaatcgga gtttgcctgg
     1681 gtcgagttgc ccgagcgtga acgcgccggg ctcttcacac gcgcggcgtt tctctcggcc
     1741 tacgccggag cggagtacca atccccgatt cgacgcggcg tgttcctgtt gcgcaaggtc
     1801 ctgtgccaga ccttgcccga tccgccgccg gatgccgaca atacgccgcc cgcgccgagc
     1861 cgcggggacc aagcccgcac tatccgcgag ctcacggaga tcaagacgag cgggggctct
     1921 tgcacgagct gtcactccgt gatcaacccc ttcggctttg ccttcgagca ctatggagcg
     1981 atcggccagt accaagccga agagaccggc acgtttcaag gtaaaccgta cgagttgccg
     2041 gtgaacgcga gcacagaggt acgtggcgga gacgtgcacg gcgcggtcac tgacgcgctc
     2101 gagctctcgc ggctgctcgc ggacagtgcg caagcgcacg actgcgccgt ggatggttgg
     2161 ttcgaacgcg cgctctcccg gcgcgccatg tcggacgatg cctgctggct gtcggccgtc
     2221 aaagagcagt ttcgcgcgag ctcgaacctc aaggaattgg tcctagcact cgcgtcgagt
     2281 gacgcggccc tgcacattcg ggaggcaaca ccatgaagat ccagcgtcgt acgttactcc
     2341 gcggactggg cggtctcgcg ttcgggctgc cctggctcga gtcgctcaac tctacggcac
     2401 acgcacaggc agccggtggc cccaagcgct tcatcgtgtt cttcgagcac ggcggcacga
     2461 tcagcgcgaa tgacaaggac ggcgataagt atgacggcaa cggtgcgaac aacggcgtgg
     2521 acggctgggc gccgataggc ggagagaagc tcgaactcgg cgcgatccac cagcccctcc
     2581 aggctcacct cgattcgttg ctcgtgctcc gcgggatcaa caaccgcgcg tgtcagcggc
     2641 agtcgcccta caacggcgac cacggctggg cgaacgtcac ggcgctcacc tccgccaacg
     2701 ccacgcagct cggcgacaag gacgaggaca tcgtctcgga agggccgtcc atcgacgcgg
     2761 tgctggcgga gcgcctcgcc gagcgaaacc cggtggcttt tccgtcggtg aacctgaccg
     2821 taccagccca caactatgga acgccgttct ttcgcgcggc gcgccagccc gtggaaggcg
     2881 agtacaaccc gatgcgtgcg ttcgacaagc tattcgccgg ggtgaccgaa ggagcgcagc
     2941 cggaccgggc tgcgctccgc gcgcgcgcct tgaaggcgag tgtgctcgat ggagttgggg
     3001 aagggctctc gctctttcgc gggcgcctga gcgccgcgga ccgcatcgcc gtggacgcgc
     3061 acctcgagca catccgcgga ctcgagcgtc agctcgagtc catcgcgcca gcggtcggat
     3121 gtcaaaagcc gaccttgagc ggtctcgaca agaactggga ctactactcc gtcgacatcc
     3181 agcgctcggg gccggcccaa atcgacatca tgctcgcggc catgcgctgc gggctcacca
     3241 atgtcgcgac cttgaatatc ggcgacttct acgcaaagtt cctgaatccg accttcccgg
     3301 cggcgtacga catcggacac tccctgcacc acagcgcgcg cgacgttggc aaggccgggc
     3361 ccgacggcgc gaagttccag gcctggtacg acacgatgct cgcgaaccgc cgctggcgta
     3421 tggggctgct ggcgcggctg ctcgacggac tagcgaatac ccgagagggt gacggaacca
     3481 tgctcgataa cacgctcatt ctgtgtgcga gcgagttcag ctgcggagcc gatcactcgg
     3541 tcgccgacct gccgattctg ctcgccggaa aggccggtgg acgcttgcga acgggccggc
     3601 atgtcaactt caacagcaag gcatccgcgg atcctcagac acggagctac gaaacccggg
     3661 cctctctcca caacttgttt acgagcgttc tccagctatt cgactttcca gacgctcatt
     3721 tcggctcgga ccatgcctgg gagcgcgggc cgctcgcggg tctgagctga gcccccctgg
     3781 tccgcatcgg gcagctcgag tgttggtgta ggtgtagaat gcttcttctc tcactcgagc
     3841 ggcggcaatc cgagagtcag aatgctaccc ttggacgaca ttcggaattg gccagtgagt
     3901 cttggcgctg cggcgcgccc gatttccgaa ggcgacgatt gatgcgacag gttcgtcgag
     3961 ctctctttct agtacccgta ccgcttcttt ctgacgcgag cgaagcgccg catgaagtga
     4021 gtgcgcgcag cgtgttgacg cgggcgctcg acggggaggg ggctgctatc gaggagctga
     4081 tagcagagct gacgccgcca ctactgcgcg ccgtacgtgc catgcttggg cccgagcatc
     4141 ccgacgttga agatctcgtt caagatacct tgattgacct ggtcgacgcg ctgccgagtt
     4201 tccgtggcga gtgcacactg ctccacttcg caatccgcat cgcggcgcgg aaggcgagct
     4261 cgagcaggaa gcgcgctcgc gccggccgtg gtttcgtcga ccgcttctgg cggcgggagg
     4321 caccgctgca ggccgccgtc gtgaatccac gcgaggcatc gctcgtcgag cggcgccggc
     4381 aactgctcga ctcgctgctg gccgagttgc cgcaggccca ggccgagacc atgttactcc
     4441 gctcggttct cggttactca ctggaagaag ttgcggccat gacgcgagtt cctttgaaca
     4501 cggtgcgcag ccgaatgcgc ctcgccaagg agacgctgcg taggcgtatc gagaccgacg
     4561 agcaagtccg tgaactcttg gggggtgaac tatgaccgcg cgcgagagtc accccgaggg
     4621 cttgattgat cgcgcgcgtc gcggcgccgc aaccgaaccc gagctctgcg cgctcgccga
     4681 gcacctcgcg ctctgcccga gttgccgttt cgagagcgcg ctccggagcg acgcggatcg
     4741 cgcggggctc ccgcgcgtct cggatgagct cctgatcgcg cgtctgcgac gaagtaccac
     4801 tcgcgccctg gttgcgcgcg gcgctcgacc gcggcgcgcc catcgcgcgc gagcggtgtg
     4861 gctcgtcgcc gctgcgttgt gcgtcggcag cgcgagcctc gccggcgtac tcggttatcg
     4921 ggtcgtctcc cgagcttcgt cagcaccacc gatggccagc ccgggcgccg tggagaagaa
     4981 cccaccgcgg ttcgccggac gatccgctct cccttcttcc tcttcgctcc ctgcgcctgc
     5041 cgatgtggaa gtggttgcgc cagtggaagc ggccgtgccg gtcgggagca taccctcgac
     5101 cgcatcgagc ggtccgccgc ccgcgaagcg ctctgcggca gagctatttg ccgaggccaa
     5161 tcgcgctcgc cgtgcgggag acatcgcgca ggccgcacgg ggctaccgcg agctcactca
     5221 gcgcttcccg gcttcttcgg aagccgcagt ggcgcgcgtc agctttggcc ggcttttgct
     5281 cgatcgcctc ggtaatgcca agggcgcgct ggccgagttc gagggctact tgaacaacgc
     5341 cggccatcgt gcgcttcgag aggaggcgct gattggtcgc gcggtcgcgc tcggccggct
     5401 cggtcgcgtg aaagacgaac gaaccgcctg gcaaacgctg ctgcgctcgt tcccgagctc
     5461 cacttacgcg acccgtgccg gagctcggct gggagagctc gacgcgcgcg ccgccgcacc
     5521 gtgaagatca tgagagcttg gccgcgatgc atggtgatcc tgttcggcgt gtgcggtttg
     5581 ctctgggtcg cgccgagccg aggtgccgaa cgcacggtgc gcgttaccat cgccgcgccg
     5641 ccggcagaag cgagcgggct cgaggccgta tttgcggagc tcctggcacg gctccacgtg
     5701 agcatccagg cgacgaacga gcgtgtgctc gatgcggccg ctatcgcggc tcccaaaaaa
     5761 ccgacccccg agctcctggc gcacgtttgg gtggatctta cgagacgtgg cgcggcgacc
     5821 ctgtacctgc tggacgtggc tacggatcgt ttgctcgtga ggaagatccg ccgcgatccc
     5881 ggtggtggtg agctggtgca agaggagctc ggacacatct tgcagacgtc cgtagaaggc
     5941 ctgctcgcgg gcgagctagt gggcgtgccg cgccacgaga tcttgccgtt gctcgaaccc
     6001 ccggctcagc cttcgactcg aaccgagaac gagtcgccag cgtctgcgac cgggtccgtc
     6061 gcatctccca cgcgcgccga gtcgagcctg cggcgcgata ggcgagcctc gctcgccgat
     6121 gcgcccgctt cgcgcggtga cgggcctgct tcgcgcatgc tgggcgggca cgccgtgctg
     6181 ctctacggcg ccgcgctatt cggccccgac gtgccgattg tgcacggccc cacactaggc
     6241 gcctttgctc acacgggagg caaactcgaa ctcgggttgt ggttgagctt gcagctgcgc
     6301 ttgccggtgg aggcgtcgaa tgggccagtt tctgctcggt tttcgcaact ctcgccccgc
     6361 gccttgttcg gtctgaggct cgtcggagac gcggagcgcg cgctccgctt cgggatcggt
     6421 ggcggcgtcg acctcgtgcg cgtcgagacc cattcgcagc cggaggcgga cgttcgagtt
     6481 gtggatcccg cgacgcgcgc ctttgcgatc ggccgagctg gcctgctctt cgattggcgt
     6541 ttctcgggcg cgctccggtt cgtgggggag ctggacgtag acatcgacag cagcgccacg
     6601 cgctacgtct acctttcacg aaccggagag cgcgtgttgc tcgagcctta cgtgctccgg
     6661 ccggcgctcg cgctgggagt ggcgactcca tgatgctgag tacgcggctc ctaaccgcca
     6721 gcgttgcgat cgtcgcgcta gcgtgctcgt ccggcacgca gacgttggcg tgggaaggag
     6781 aagggcaagg gagtgcgggc tcgcccggca tcgtggatcc ggcgtttccc gaggatctac
     6841 tcgagccctg gcacggcggt cccagttact acggtaagtg gccgcgcggc ctttggacgg
     6901 acacggcgca cttcccgatc ggagtctgga tgcagaaccc cgacaacgcg caacgtttcc
     6961 gcgacgtcgg catcaaccta tttctggggc tgtgggaggg gccgaccgat gagcagctcg
     7021 cgggcttgct cgagcaaggc gtgcgcacca tctgtgacca gaagggcgtc tggcaaagcc
     7081 atctcgaaga ctcgactatc gccgcgtgga tgtccgagtg gggaccggat aacgcccaag
     7141 agcgtgcggg agagggctac gatccctgca tccctccgag cgacgtgacc gcgacgatcc
     7201 gggaaatagc cgcgcgcgac gcgactcgcc ccgtgttgct tggcttcggg cgcggcctcg
     7261 ccgatcccga gtgggtgggc cgcggagaat gcaccggtcg tacggacatg taccctgagt
     7321 acgcgcgcga ggccgacgtc gttggctttc attcgtaccc ggtgaactcg agccaacaac
     7381 tcctgctcgt ggcgcagggg gtcgacaact tgcgcgagta caccgaccac cgcaaacccg
     7441 tgttcgctct gctcgaggcg gcgtccatcg acggcaaggt ccgtcccacg ccggagcaaa
     7501 tccgcgcgat ggtgtggatg gtcttgatcc acggcgcggc gggcattcac tactactgtc
     7561 accactatgc ggatccgttc aacgagacgg actgcctgga cgacgctccg acggcgagca
     7621 ccatcgcctc tctcaaccgt ttgatcgggc agctcgcgcc cgtcctcaat cgtcccacgg
     7681 tgacgaatgg ggtcatggtt cggctggtga attcaggcaa tcgagtcgac gtgatgctca
     7741 agcggcaggc ggacgccacg tacctattcg ccgtggaaat gctgggacgg gccacatccg
     7801 tgacattcga gctccaacgt ttcccggccg ccgcccaggc ccaggtgctc gagtccgagc
     7861 gtagcctgga cgtcaccaat ggcgtgttca tcgaccagct tccggcctac ggcgtacggg
     7921 tgtaccgcgt cgtgccgcgc tgagttgcag tctcacgcgt tgcgccgctg cgtgcgcgag
     7981 gtagacaacg tgggcgagag aacgtgatac gatgttcgga tggtgatcac ttgatatcgc
     8041 gagatcgaaa accctgggtc gcattgagtg tgagtgtggg tgtgagtgtc gcggtgctct
     8101 gcgcgggagc ggcctgcagc gagtcggagg gttcggccac acccccgacc cctccggcgg
     8161 tgctggcaca acctcgacca gccagggcgc ggcgagcggt cggggcggcg ccgccgcagg
     8221 tgagggcggg caggctacgt ccggaggtgg tcagagcggg aaaggcgggc aggcgagtgc
     8281 tggtggggcc acggctgggc acgccgggat gacaaccgct gcggtttcgg gatcgctggc
     8341 tgagcgttac ccggaggaca atggcttggc gtcggacccc gccgtgctct tctacgacga
     8401 cttcgaagca ggctggggac gctgggatgc tcccaagcaa gacactcagc atctctttgt
     8461 cgagtccgat gcggcgcgcg cgcacgctgg ttcccgcttt ctacggtcca cggtcacgga
     8521 ggctcagctc gcagagcaag agtacatctc cgcggcgccg cgcgcggatt tcacgcgacg
     8581 ggtccctacc gtgtattggc gcttctacgc tcaattcaag ggagcttccg cgactcccca
     8641 tcattgggtg cgcatggcgg cgggcacccc cgagtatgcg tcgagtggtc tcgccaacac
     8701 cgttccgcca ggcgatcgag gcttctggtt cgacttcgat gcggacacgg gagatcactt
     8761 caacttctac gtttactggt tccaaatgcg ttcgggtcgc tgtaatgacg gcagcgtcac
     8821 gccgggatgt gccggcgatc agggcacgac ctaccattat ggcaacacgt tcgctcctcc
     8881 cggacaagat gagtttccga gagatcgctg gttctgcgta gagatcatgg ccaaggccaa
     8941 tcaagtaggg caatcggatg gcgaacttgc attctgggta aatgaccagc tgatcgggga
     9001 ctaccgacct ggctttccga atggcacttg gctgcgtgac aagttccaca ctgacgggtg
     9061 cacgttctcg gcatgtacgg agccgaagcc tttcgaaggc ttcgagttcc gatcgagcga
     9121 cgacgttctc ttcaagagct tcttcatgga cgcgtattac gagcgcgcca cgatggagcg
     9181 ccgacgagcg gaattggaag cgcgcgggct cacggtaccg agcgagcaga cgattctcta
     9241 cgacgacatc gtagtcgcca cgagtcgtgt gggctgcaga agaacacgcg agtgacaggg
     9301 agccagtgca ggggcggtcg cgtctcggga gcccaagccg cagtcgagat tgctctcacg
     9361 ctcatgaacc gccctcgcga ttcgatatct gttgctcggg ctctggccgc aaggctaacg
     9421 cgtccacgtc gtgagcagcg ttgcaccgtc catgtagttg ctcgccgtga tgcccaggct
     9481 ctggttggcg atattgccgt ccatcgcgtc gcctgcgccg cggaccacga aaggtcgatg
     9541 gtacagctcc accttgctga ccttcttgtc gccgcgcacg tttacggcaa aatacgtcat
     9601 gtctcggcgc cagggccccg agcctaaggt ccaatcgcgc ggcggtcctt gctgcgccac
     9661 gagcgccacc tgttcgctgt cgtcggcgta ggtcactttg acggtcaaat ccttcggcca
     9721 atagaagtac ttgcggtaag ggtccttcga caaggcagcg aaggtcgtcg gatccgttgg
     9781 atcgacgatc tttggcaatg aaccgctgaa ttcaatgggt ggatacagga tattggccgc
     9841 ggcgacgctg gggtggtagc tgccgaagat ggtcgccacg ggcacgtccc actcttgcgc
     9901 aaacaggata ttcaaggact cttcgtcggt gacggtgccc gagacccgtt gaacggcgcg
     9961 tacgccgctg gcgtccatca ccaggttcgg atagcccttc gtctcaccga tatgataggg
    10021 cacgctggcg ccacggtagg agaccttgcc agtcagatcg gggccgccgc gcaagttgtg
    10081 aagaatcgca aacgccgaga aatcaccata gccgtcccaa ggccctttgc ccgtcgatcg
    10141 ccactcctcg caggagtgcg tgcgctgcat cgcatcggag cgctctttgc cgtacacctt
    10201 ggtgttcttc tcgtcctgac aaatcggcga cgtgaattct ccgatattct gataataatt
    10261 ccaagtctcc ccgcgaccgc cgccgttatc ggtgttcccg ccgtaggggt acagatactg
    10321 ggagtccttg ccttgaaagg gcccttcgcc ccagtgctgc aagccgaagg catggccctg
    10381 ctcgtgaatg aagacatctc cgaagtccgc acccacgaag ttcttgccgc cgccccagcc
    10441 acccggaaag aacccctccg agttcccgta atagaaggtc acggccaaat cgccattggc
    10501 cgcatggatg gcttcgatga tgttgatcgt cgtcccgagc aactccatgg gctcgacgtc
    10561 actgctcata caggtggcgc ccgcggcttc gtcatcatcg cagagccgcc gatccaagac
    10621 gacgggcggg gcgccctctt cggaccctgt gtagtgtgag gttgcgatct tcggcagtac
    10681 ggcgcgtccc ggaatcttgc cgaagcgaac gcgcgctgcg ggcgttgcgc tcgcgaagtc
    10741 accgagccag ctcgcgggag gcggcttgtc gctgtgctcg tcgttgtaat tgagcacatc
    10801 catcgggagc atgactagat tgacgtccgg cgtagcggcc acggcgagct gctcactgga
    10861 gaacgtcttg ctggacgcgc cggcggtaac cgtgagactc aaacccgggc gcaaccaggt
    10921 ggacggcagc gtggcggtga aacgatccgc cttcgtatgc ttggtcgcgt ccacggttgc
    10981 cgcgagagat gcggggcccg ccaaacagag cggaccaagc ttctctaccg tcgtggaacc
    11041 gggcttgctc accgtgcccg acacgcgtac gtccggagcg gccccatccc cgctgaccag
    11101 aacttcgacc agcgcgggac gatcagcagt tagatagaag aagggatgcc ccggctccag
    11161 cgcgtgcgtt tgagcgaaga atacccgctc gatcttggcc gtctggttag cgcgctccac
    11221 gcaagcggcc gcatcgttgg gctcgggaaa tgagtagagc tcgctcccgt acgagcgagg
    11281 gagcggatcg cggaccgtcg cgccgcctgg agaaccgcct tcggcgcccg cggcgttgcc
    11341 gccggttgat gtggaacccg cggttccgcc cgacgcgtgg gcgacacctc cggtgccatt
    11401 accaccgttt ggcacgccgc tagttgctcc gcccgaagag tgtccgagtc cgccgctacc
    11461 agccgtggtc cctgtctcgc ggcctccagt ggtctcgcgg cctccggtgg tctcgtggcc
    11521 tccggtggtc tcgcggcctc cggtggtctc gcggcctccg gagcctccag cgctcgaaga
    11581 ggagccgcag ctcgtcagcg tcatgagcgc agtcgcgaaa cagagatccc gggcggtcac
    11641 gaagcaccca aacacacatc gagacatagc gcccaatata cactccaagt cgttgcgcaa
    11701 gagacacgac tttgtgccca cattaggcga tgtaagccaa aggggttatg ggtgcttcgc
    11761 gctgtaggtc caagcgccca gtgtctcgtt gatcactcct cgcacgaatc accgtcgact
    11821 ctgaccgaga cgaaacgatg accgatgccg gaagacctca ccattcgtcc cgacccgcgt
    11881 cggccaggtg cacgcggcag ttgtggagct tctctcgatg gcggcccggc aggtgcacgg
    11941 gcgcgacgca cgccgcgtgc acctgcgagc gcagacactg cgctccttgt agctccaagc
    12001 accgcgctcg ccgcgcactg caacggcagc aggcctagct ccccgaaccg tcgtgtcgtg
    12061 cgggtaacgg tcgagagccg cggcgcgttc atttccctcg tagcagcccc gctacgcccg
    12121 cacaatctcc gatcaccgcg gtcagtgtcg caaccgttag accgtgacgc tctgcgaccc
    12181 aggttgccgc gtcgtgcgat cgggcgagct gaattccagg tcgcccgaga cgcagccgct
    12241 gcgaacgctt gagttcgatg gcgaaccagc gggaatgagc cgcatcacca gagcgatcca
    12301 cggcaagaaa gtcgcccgcg gcatgccgct ggtgagttcg aaataaccaa tacctagttt
    12361 gctctcccaa caactgccgc gcgaaggaaa gctccgcgcg acaaagaccg aaaccagaat
    12421 ccaggcgact caccaggact cgccgaccag acgtggtgac aaacaagcct ccgcgcgcca
    12481 acaccaagcg gacggcagga cacactatcg atgccaaagt aaccccttgc gctgcgcaca
    12541 gcgcgcacga accaagcccg ttccacgcga tcggcgaacg ccgctcgcgc ttcagacgac
    12601 tgctgggatc gggatcagac cttcaaggga gtacctgcgg cgtcggcgaa ctcggagtcc
    12661 ataccaggtg taagcgcgtt gtccatccgg tctggttcga gttccgcctt caaggtctcg
    12721 ccgcgcttcg ccaacgccgt ctccccagca ctctttcctg ggtaccccca acgattcctc
    12781 aaggccgctt cggttcgtgc aactacccac gtcgggcaaa agctcggcgc ccccctcggg
    12841 agccgcgaaa ggctcgctgc tacttcatga tcctggcaca acccgacaca actgggctct
    12901 tggcacagaa gggggaggca actgacccgg cgggtccgcg aactgggagc ggtttgtggg
    12961 cagcaaacca atccaaggcg ggcatgtgtc ccgtcgcgtg cccgtggcat tggctttgca
    13021 caccgtgcgc gcgtctactt ggaggcactg agactggcgc tggattga
//
LOCUS       DASTKU010000736         6727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1035355, whole genome shotgun sequence.
ACCESSION   DASTKU010000736 DASTKU010000000
VERSION     DASTKU010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035355"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1715)
                     /locus_tag="VFQ61_25870"
     CDS             complement(<1..1715)
                     /locus_tag="VFQ61_25870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957962.1"
                     /translation="MSHAKSPSLRLAMTTAVLALAHQVGAKTVREGVFLSTYSSANLP
                     TAMFGSALCSIPAAFVVSRAMTRWGPQRAAPALFAASALLWLLEWFFLPTARGLVAAL
                     LFLHVSVGGALLVSAFWSVVSERFDPHALKTQVTKIGAAATLGGLLGGALTERTSRYF
                     HTRSTLLLLAGICLTAALFVWRLSQRRISVHEESVATGSPRLTPYLRNTALLVTCAAL
                     AGTFTDFALKNAAAQHFTRAEDLVRFFALFYTGSSLVGFLLQAFIAPRLLSTFGIGAT
                     LSATPLGVLLLGTAAALSPSMVMLGTLKAAEAALSSSLFRSAYEPLFTPLASATKRRA
                     KSLIDVVFDKGGDALGALVIMAILWGGPTLLTRAPASLAALAAAAGLHLALQAQRGYV
                     TELEARLRTGTLSVRPDELTDPAAKLALSATAVGLDRTKLLEEIRRLNENTNAESKHL
                     ADQADRLSEELRVLLGSDFAAAERVLGKRDLDPRLAAFVIPLLGVRPLARAAAEALRR
                     LGPMAVALMAEGMLSPDLPVVVKRRIPHVLRSLKGEAVVEALTQALRAGEFSVRTRAA
                     LALREV"
     gene            complement(1712..2791)
                     /locus_tag="VFQ61_25875"
     CDS             complement(1712..2791)
                     /locus_tag="VFQ61_25875"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9957963.1"
                     /translation="MILRDPRAVSSDAPALALGVALLLGASSGRAEQPLPPRPAGSDP
                     SVVHYARSTSPTSPRSVATAADTDAVCEPTPAPSRFDWKPEWRRSSAINYAVIGAGFS
                     TAIVLELLPQRRHGSWSGPILLDDSLRSSLVASTSEGRNRAGRISDIGQWGSVAHTVF
                     DATFLTMWHHHAPDLGYEMLVMNAEAYAVSALLNSVTKRISGRERPDTTECLASAGYD
                     STCATNDRYASYYSGHTSITATSAGLTCIHHIQNPLYGSPWDAAACAAGITLTSFTGA
                     LRVVADRHWASDVITGHMVGFTTGYFLPLIFHYRGVPRGKPAPDSSWRRDEARRASPS
                     YVPVIQGLLPSFDQSGARLILFGSL"
     gene            complement(2778..4493)
                     /locus_tag="VFQ61_25880"
     CDS             complement(2778..4493)
                     /locus_tag="VFQ61_25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957964.1"
                     /translation="MVFERSTSSFVARSGLAALALLLSCCGRAPRFPLREPLERDPDA
                     RPFAPQPAEYYSPFAWDGANQLVFRPISRFFAVDPAGPAANVNALDEVPDSSWFTNRL
                     GAHALTPEDVARGSCPAQSLDPLAPDGAWLIDKGKANGANPGFRIRVEGLGKFMLKSD
                     PPSEPERATGATAIAARIYHAIGYHAACDSVVYFRPEILRLKPGLTVTTNTGVTHPLD
                     REHLNEMLAGASRRNGRVRMVASAWLPGNTIGPYKYDGLRDDDPNDIIPHEDRRELRG
                     ARLIAAWLNHFDSREQNTMDTFITPDGAPPGSGYVRHYIIDMGDCFGSVWADDGISRR
                     LGHSYVLDFGYVAEDFVTLGAISRPWERARRTAHVFNYFSARDFDPESWRGEYPNPAF
                     MRMTEADGAWMARILARFTDPLVAAAVRAGQYEPAAERYLTQVLIARRDAILRRYLSR
                     LSPIGDVSLSGSTLCGTDLARNAGVIPNESLSFRVSVYAGAQLAPVRDFRVTSPQPAQ
                     LCVEISHRNLPSQISPDDARRYLVVDLDNGYARGPLRVHLYDLGQSRGFSIAGIERPE
                     SHDPP"
     gene            complement(4483..5856)
                     /locus_tag="VFQ61_25885"
     CDS             complement(4483..5856)
                     /locus_tag="VFQ61_25885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957965.1"
                     /translation="MALRSWIFVALSGLALCRSAQADENRKRPLPDYDGRPDARRPAD
                     VVLWIPRLLLSPAYVVAEFVIRRPLGFLITEAERAQLPSALYDFFAFGPDHKAGFTPI
                     AFVDFGFEPSVGLYAFWDDAGFKGHDLRVRGSTWGSAWLAGTVTERFRFSEHQSLTLS
                     GTLIRRPDYAFYGIGPDSRESQISRYGSDRADARVLLRTALGGANLIETSVGYRGARF
                     RPGAYDEDPALPVRVSEGAFALPPGYQDGYRAVLSALRVVLDTRTESSEANRATQSDE
                     YPLRSFEDGARLELEVEHGTDPGTSAPAGWLRYGATIGGFLDLGHRNRVLSLSFAALG
                     ADALGARPVPFTELVTLGGPMWMPGFRAGRLYGESALLGTLRYSWPIWMWLDGSLQAS
                     VGNVFGEHWAGASLARSRFSGALGLETNNSRDSIFQALVGFGTETFESGASVNSIRVA
                     LGARHGF"
     gene            complement(5858..>6727)
                     /locus_tag="VFQ61_25890"
     CDS             complement(5858..>6727)
                     /locus_tag="VFQ61_25890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957966.1"
                     /translation="SAGLIPLLGLRALLWVALSLLLVCAGLYWAVERRQRALGVAPRT
                     NAQAPASPAARAPNESPTKPSGAFALVMRDRYLSLIAAFSLIFTWVNTNGEYMLGKVI
                     RQHAVDAVAQGRAHSVGEQIGASYAQFFFYVNVIGVLLQTFVVARLVRWLGFRTAFLV
                     LPVLALGDASAIALAPVLLVICVAKGLENSTDYSLNNTLRQMLWLVTSREMKYKAKQA
                     VDTFFVRMGDVCSALLVWIGVTYFALDVRRMATLNLLFALVWLFLAVVIGREHARRAR
                     AVALSGTPSTTGQ"
BASE COUNT         1307 a         2121 c         2234 g         1065 t
ORIGIN      
        1 acctcacgca atgccaacgc cgcgcgcgtg cgcacgctga actcgccggc acgcagggct
       61 tgggtcaagg cttcgaccac ggcttcgccc ttcagcgagc gcaatacgtg cgggatacgg
      121 cgcttcacga ccaccggcaa atcgggcgac aacatgccct ccgccatcag ggcgacggcc
      181 atcggcccca accgtcggag tgcttcggcg gccgcgcggg ccagcggcct cactccgagg
      241 agcgggatta cgaatgccgc caagcgtggg tcgaggtctc gtttgccgag cacgcgctcg
      301 gccgccgcga agtcactgcc gaggaggacg cgcagttctt ccgagaggcg atcggcctga
      361 tccgcgaggt gcttggactc agcgttggtg ttctcgttca ggcggcggat ctcttcgagt
      421 agcttggtac ggtcgagccc gacggcggtc gcggacagcg cgagtttggc cgccggatcc
      481 gtgagctcgt cggggcgcac actgagtgtg ccggtgcgga ggcgcgcttc cagctcggtg
      541 acgtagccac gctgggcctg cagagccaag tgcaatccag cggccgcggc cagcgcagcc
      601 aagctggcgg gtgcgcgcgt caatagcgtg gggcctcccc aaagaatcgc catgatcacc
      661 agggctccga gtgcgtcacc tcccttgtcg aacacgacgt cgatcaacga cttggcgcgt
      721 cgtttggtgg ccgaggcgag gggagtgaag agcggctcgt acgcgctgcg gaacaaagaa
      781 gagctgagcg cagcttcggc ggctttcaaa gtgcccaaca taaccatcga aggtgacagc
      841 gccgccgcgg tgccgagcag caacaccccc agtggggtcg cagaaagggt ggcgccgatc
      901 ccgaacgtgc tgagcagccg cggcgcgatg aaggcctgca gtaggaagcc taccagcgaa
      961 gagccggtgt aaaacagcgc gaagaagcgc accaaatctt cggctcgggt gaagtgctgt
     1021 gcggctgcat tcttcaacgc aaagtcggtg aaggtgccgg ccaatgccgc gcaagtcacg
     1081 agcagggcgg tattgcgtag atacggtgtc aggcgggggg agcccgtggc gacactctct
     1141 tcgtgaacgc tgatgcgccg ctgcgatagg cgccaaacga acagcgctgc cgtaaggcaa
     1201 atgccggcta gaagcaacaa ggtgctgcgc gtgtgaaagt agcgtgaggt gcgctcggtc
     1261 aaggccccgc ccagcaagcc acccagcgtt gcagccgccc cgatcttcgt gacctgcgtc
     1321 ttgagcgcat gcgggtcgaa gcgctcgctc accaccgacc agaaggcgct gacgagcagc
     1381 gcacctccca ccgacacgtg caaaaacagc agcgccgcga ccagaccccg agccgttggc
     1441 aggaagaacc actcgagcag ccagagcagc gcactggcgg cgaacagcgc gggggcggcg
     1501 cgttgcgggc cccaacgcgt catcgcgcga gacaccacga acgccgctgg gatcgagcac
     1561 aaggcagagc caaacatggc cgtgggcagg ttcgcgctcg agtacgtgga aaggaacacg
     1621 ccctcgcgca ccgtctttgc gcccacctgg tgtgcgagcg cgaggacggc ggtggtcatg
     1681 gccaagcgga ggctggggct cttggcgtga ctcataggga tccgaagaga atgaggcgtg
     1741 cgcccgattg gtcgaaggac gggagcaagc cctgaatgac tggcacatag ctaggcgatg
     1801 cgcgccttgc ctcgtctctc ctccagctcg agtcgggcgc tggtttgccg cgcggcactc
     1861 cgcggtagtg gaagatgagc ggcaaaaaat aaccggtggt gaagcccacc atgtggcccg
     1921 tgatcacgtc cgatgcccag tgtcggtcgg ccaccacgcg cagcgcaccc gtaaacgacg
     1981 tgagcgtgat gcccgcggcg caggccgctg catcccaggg cgatccgtaa agtgggttct
     2041 gaatatggtg gatgcaggtc agccccgcgc tcgtcgcggt gatagaggta tgcccgctgt
     2101 aataggaagc gtagcggtcg ttggtggcgc aggtcgaatc gtagccggct gaagcgagac
     2161 actcggtggt gtcggggcgc tcgcggccgc tgatgcgctt ggtcacgcta ttcagcagcg
     2221 cactcaccgc gtacgcctcg gcattcatca ccagcatttc gtaacccaag tctggagcgt
     2281 ggtgatgcca catggtcaag aaggtggcgt cgaatacggt gtgagccacc gagccccatt
     2341 gtccgatgtc actgatccgc cccgcccgat tgcggccctc cgacgtcgag gcgaccaggc
     2401 tggagcgcag cgaatcgtcc agcaagatgg ggccgctcca gctgccgtgt cggcgttgcg
     2461 gcaacagctc gagcacgatc gcggtcgaaa accccgcgcc gatgaccgca tagtttatgg
     2521 cgctggatcg acgccactcc ggcttccagt cgaaacgtga gggagccggc gtgggttcgc
     2581 ataccgcgtc ggtgtcggcg gccgtggcga cactgcgtgg actcgtcggg ctggtgcttc
     2641 gcgcgtagtg aaccacgctg gggtcggagc ctgccggacg gggcggcaga ggctgctcgg
     2701 ctcgacccga ggacgcgccg agcaagagcg cgacccccaa cgcgagggca ggggcgtccg
     2761 acgacaccgc gcgaggatca cggaggatca tgagattcgg ggcgctcgat gccggcgatg
     2821 ctgaaacctc ggctttgtcc gagatcatag aggtggaccc gcagcgggcc ccgagcgtac
     2881 ccgttatcca gatcgacgac taggtaccgc ctcgcgtcgt cgggcgaaat ttggctgggc
     2941 aaattgcgat gcgatatttc gacgcagagc tgggcgggct gcgggctggt gacccgaaaa
     3001 tcgcgaactg gtgcgagctg cgcgcctgcg tagaccgaca ctctgaacga caagctttcg
     3061 ttcgggatca cgccggcatt gcgagcaaga tccgtcccgc acaacgtcga gccgcttagg
     3121 gagacatcgc cgatcggaga caaccgcgac aagtagcggc gcagaatggc gtcgcgcctc
     3181 gcgatcaaga cttgagtcag gtaacgctcc gccgccggtt cgtactgccc agcgcgcacc
     3241 gcggccgcta cgagcggatc ggtgaagcgc gccagaatgc gggccatcca cgcgccgtcg
     3301 gcttcggtca tgcgcatgaa agccggattc ggatattcgc cgcgccaaga ctcggggtcg
     3361 aagtcacgcg cgctgaagta gttgaatacg tgggcggttc ggcgggcacg ctcccagggt
     3421 cggctgatcg cgcccaaagt aacgaaatcc tccgccacgt agccaaaatc gagcacgtag
     3481 gagtggccca gccgccgcga gatcccgtca tcggcccaaa cgctgccgaa gcaatcaccc
     3541 atgtcgatga tgtagtggcg cacgtacccg ctgccgggcg gcgcgccgtc gggggtgatg
     3601 aacgtgtcca tggtattttg ctcgcgcgag tcgaagtgat tgagccacgc ggcgatcagg
     3661 cgagcgccgc ggagttcgcg tcggtcttcg tgcgggatga tgtcgttcgg atcgtcgtct
     3721 cgcaggccat cgtacttgta gggtccgatg gtgttgcccg gcagccacgc cgaagccacc
     3781 atgcgcactc ggccattgcg gcgcgaggcc cccgcgagca tctcgttcaa gtgttcgcgg
     3841 tcgagcggat gggtcacccc cgtgttcgtc gttacggtga ggcccggttt cagccgcagg
     3901 atttcgggtc ggaagtatac gacgctgtcg cacgctgcgt ggtagccaat cgcgtgatag
     3961 atgcgggccg cgatagcggt ggcgcccgtc gcccgctcgg gttcgctggg cgggtcgctc
     4021 ttgagcatga acttgcccag cccttcgacg cggatgcgga acccagggtt cgcgccgttg
     4081 gccttgcctt tgtcgatcag ccacgcgcca tcgggcgcga ggggatccaa gctctgggcg
     4141 gggcagctgc cgcgcgcgac gtcttccggc gtgagcgcgt gcgccccgag tcgattggtg
     4201 aaccaactcg agtctggcac ttcgtcgagt gcgttgacat tggcggccgg tcccgccgga
     4261 tctacggcaa agaagcgcga gatgggccga aacaccagtt gattggcgcc gtcccaagcg
     4321 aacggtgagt agtactccgc gggttgaggc gcgaacggac gcgcatctgg gtctcgttcc
     4381 agcggttcac gcagcgggaa gcgcggagct cgtccacagc aactcaatag caatgccagt
     4441 gcggcgaggc cgctgcgcgc gacgaagctc gacgtggatc gctcaaaaac catgacgtgc
     4501 tcccaaggca acacgaatcg aattcacgct cgctccgctc tcgaaggttt cggtaccgaa
     4561 gcctacgagc gcctgaaaga tgctgtctct cgagttgttc gtttcgagac cgagggcgcc
     4621 tgaaaagcgc gaacgggcca ggcttgcccc cgcccagtgc tccccgaaca cattgcctac
     4681 cgacgcttgc agcgagccgt cgagccacat ccagattggc caggagtaac gcaaggtgcc
     4741 gagcagcgcg ctctccccgt acaatctacc ggcgcggaaa ccaggcatcc acatggggcc
     4801 gcccaaagtg acgagctccg tgaagggaac cgggcgtgcc ccgagcgcgt ccgctcccag
     4861 cgccgcaaaa gaaaggctga gaacgcggtt gcggtgccct agatcgagga agccgccgat
     4921 cgtagccccg tagcgcaacc atcccgcggg ggcgctggtg ccggggtcgg tgccgtgctc
     4981 gacctccaac tcgaggcgag cgccgtcttc gaagctccgc aaaggatact cgtcggactg
     5041 tgttgcccga ttggcctcgc tgctctccgt tcgcgtgtcg agcacgacac gaagtgcaga
     5101 caatacggcg cggtagccgt cttggtatcc cggaggcagc gcgaacgcgc cttcgctcac
     5161 ccgcacgggt agcgccggat cttcgtcgta cgctcccggt cgaaagcgtg cgccacggta
     5221 cccaacgctg gtctcgatta agttcgcgcc tcccagagcg gtgcgcagca gtacgcgcgc
     5281 gtccgctcgg tccgagccat aacggctgat ctgcgattcg cgagaatctg gtccgattcc
     5341 gtaaaatgca tagtccggac gccgaatgag agttccgctc agggttaagc tttggtgctc
     5401 cgagaaacgg aagcgttcgg tcacggttcc cgcaagccag gctgagcccc aggtagagcc
     5461 tcggacccga aggtcgtgac ctttgaagcc agcgtcgtcc caaaaagcgt acaaacccac
     5521 gctgggttcg aagccgaaat ccacgaaagc aatcggcgtg aagcccgcct tgtgatcggg
     5581 cccgaacgca aaaaaatcgt agagcgcact cggcagctgt gcacgttccg cttccgtgat
     5641 tagaaagccc aacggtcgcc ggatcacgaa ctcggccacg acgtaggccg gggagaggag
     5701 cagccgcggg atccacaaca cgacatcagc ggggcgtcgt gcgtcggggc gaccatcgta
     5761 gtccggtagc gggcgcttgc ggttctcgtc ggcttgggcg ctgcggcata gggcgaggcc
     5821 gctcagggcc acgaagatcc agcttcggag cgccacgtca ttgccccgtc gtgctcggcg
     5881 tccccgagag cgccacggcg cgcgcgcggc gcgcgtgctc gcgtccaatc acgaccgcca
     5941 gaaacaacca gaccaaggca aacaacaggt tgagtgtagc catgcgccgc acatccaatg
     6001 cgaaataagt gacaccgatc cataccagca acgcggaaca gacgtccccc atccgcacga
     6061 agaacgtatc gacggcttgc ttggctttgt acttcatctc gcgagaggtc acgagccaca
     6121 gcatctggcg tagcgtgttg ttcagggagt agtcggtcga attctccagg cccttcgcaa
     6181 cgcagatgac caagagcacc ggcgcgagcg cgatggcgga ggcgtccccc aacgccagca
     6241 cgggtagcac caagaaggcg gttcgaaacc cgagccagcg gaccagccgc gccacgacga
     6301 aggtctgcag cagcacgccg atcacgttca cgtaaaagaa gaactgcgcg taactcgcgc
     6361 caatttgctc gccaacggag tgggctcggc cttgagccac ggcgtctacc gcatgctgcc
     6421 ggatcacctt accgagcatg tactcgccgt tggtattgac ccaagtgaag atcaaagaga
     6481 acgcagcgat caagctcaaa tatcgatccc gcatgaccaa cgcgaaagca ccggaaggtt
     6541 tcgtggggct ttcattcggc gctcgggcgg cggggctcgc gggggcttgt gcattcgtgc
     6601 ggggggccac cccgagcgct ctttgccggc gttcgaccgc ccagtacaaa ccggcgcaca
     6661 cgagcagcag gctcaacgcg acccacagca gcgctcgcaa gccgagcagc ggaatcaacc
     6721 ccgcaga
//
LOCUS       DASTKU010000737         3151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_812134, whole genome shotgun sequence.
ACCESSION   DASTKU010000737 DASTKU010000000
VERSION     DASTKU010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812134"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..759)
                     /locus_tag="VFQ61_25895"
     CDS             complement(94..759)
                     /locus_tag="VFQ61_25895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9957967.1"
                     /translation="MNLVLFAAEGGGYGQLIEAFKHNPVFLVMNLCCSAVVLAIVVDR
                     FSFQLTRYRIDSKEFFAQIKKLVTAGNIDRAIKLCETEDYPLLQLVRAGLTQSSKGAD
                     EIDAALSEKLGELKPQAEKRVGVLWSLANISTLIGLLGTVSGLIATFGAISAPGLSQA
                     DKQRMLSNGIAEAMYNTALGLGIAVFCMITHIFLHTRAKNIQHDLEATMEKTFNLLTL
                     QQR"
     gene            2823..>3151
                     /locus_tag="VFQ61_25900"
     CDS             2823..>3151
                     /locus_tag="VFQ61_25900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9957968.1"
                     /translation="MFSALWHQYTQGGWAMWLIMFWLVCSIAVIVERTVFLFGASINK
                     DVFVATMQKCILAGDVAKAVKMCSAANAPLARIVQAGLVKVNRPDEEVQAGMDEAALR
                     EMPTINRR"
BASE COUNT          563 a          928 c         1081 g          579 t
ORIGIN      
        1 cgccctcctc gtccggcgac agctctctcg gctggctcag ccggcgaatc ttgctgcgtt
       61 gcgcggcgct gagggggacc gcttcggcca tgctcagcgc tgctgcaacg tgagcaagtt
      121 gaaggtcttt tccatcgtcg cttcgagatc gtgctggatg ttcttggcgc gcgtatgcag
      181 aaaaatatgc gtgatcatgc agaacaccgc gatgccgagg ccgagcgcgg tattgtacat
      241 ggcctcggcg atgccgttcg agagcatgcg ctgcttgtct gcttgtgaca gcccgggcgc
      301 ggagatggcg ccgaaggtcg cgatcagccc gctcaccgtg cccagtaggc cgatcaaggt
      361 ggagatgttg gcgagagacc agagcacgcc cacgcgcttc tcggcctgcg gcttcagctc
      421 ccccaatttt tcgctgagcg ccgcgtcaat ctcgtctgcg cccttactcg attgggtcag
      481 acccgcgcgg acgagctgca gaagcgggta gtcctccgtt tcgcacagct tgatcgcgcg
      541 atcgatgttg cccgcggtga cgagcttctt gatctgcgcg aagaactcct tcgaatcgat
      601 gcggtagcgc gtgagctgga acgagaaacg gtccaccacg atcgcgagca cgaccgccga
      661 acagcacagg ttcatcacca ggaagaccgg attgtgcttg aaggcttcga tcagctgccc
      721 atagccgccg ccctcggcgg caaaaagcac gagattcatt cgccgacgtc cctttcctct
      781 agtctctctg gcagtgtgac gcgctaatga gagcgtgtct gggcgatgtg ccgactatag
      841 cgagcggcag agaaactgtg caagccgggc cgcttggacg tgccgctgga cggctcggcg
      901 gcgcacgggc ttaaggggcg ccccagcgct tcagcctcgc tccgagagac ggcgcatctt
      961 ccggctacaa tgcggcgggc agctgccaaa tccagccttc gatagctcga gagctgcgcc
     1021 gggacgagtc cccccggcgg cttccgcgcc atttcccgga gcggcgggct ctcgagcggc
     1081 gctcttggtc ggcgcaacat gcgcgaagtg aggcctgcgc gcgccagcgg acggggcggt
     1141 cgccgcgaca gggccgcgag gcgcctgcgc caggcgcatt gcccgagcgc ccgcgccgcg
     1201 cgcaaccagc tgctgtcgat cgagcctctg gtgcatgcct gcgaggacgt gcttcgctgg
     1261 gtcgcggtgc cccgaccagc aggtgggcgc atgcctacct gccgcggcca tcaagcctgc
     1321 ggtggagcca ctctcagttg tgccaagctg cgtcgtccga ggtagcaacg cggcgcgccg
     1381 aaggcggtcg tgacctcgcg atactcgcgg atcgcgcttg gtgccggcgg gcggtcgttc
     1441 cgtcaatctg cttgactgat gggcgcgtgg ttgcgcaata tatcgctgga gagcggtcgt
     1501 gggagcgacc ccgcaccccg caaatgaggc gagtggcgtc gtcctgctgg agtccagtga
     1561 agccacgggc tgggcgtagt gttactcggc ggtgcagtct tactcggcaa agcagtgtta
     1621 ctccgcaaac aagtgcactc ggcggacggg ttgctcggtc aacggcactt cgaggagcgg
     1681 tgggtgcgcc cacccgcggg agctgccacg atgcgcacgc gggtgcgccg cattgggtct
     1741 gtgcgttacg ccaggtacgg tagaggtgcc gccgaggtcg ccgctgaact cgcactagcg
     1801 gcgaagcttc gcctggacgg gggacgcccc cgggcttcct tggaccaggc ggcgttcgcc
     1861 tcgatacggg agcacttcgc ggttcgcatt cggggcgctt tgaattcccg gccccgactt
     1921 ggctctgaat tcgggagccc ggcttgggat ggtggatcgg tgggttgacg tttcgcaggt
     1981 cgaggtcttg gaaattggga tattggggca gcgcggcaaa gagcactcgg cggaactggg
     2041 gcccggcgct gggtccccca tgaacgggct cgcgaagaga gcagcgaaaa agcggccaga
     2101 gctcggcgat ggcgcttgcg gaccgagcgg tggaatcttc aaaatcagac taaactagga
     2161 ctagcgtaag cgcctcggag ctgggaactt gctccccaga gttgaatgac ctcggcgaaa
     2221 ctcgatcgaa tggcagcgga gccggtaacg acgtgtcttc aggagcacct ctcggacgag
     2281 gcatcgaggt cctagcctgc tcgagagggc gcgcttgccg ggcgacggtt gcttcgagag
     2341 agttgcttcg ggagagttgc ttcgggagag ttgcttcggg agagttgttc cggagatggt
     2401 cgctggtgga cggttgctgg ggggacggtt gctcgggacg ctcgcttcgg gacgattgag
     2461 acgcttgatt gaggacgaga gcaaacgccg cgcaacggag atcgagcatc gctgacggaa
     2521 ggcctacggt cgaccgagag cctacggcgg gtcgagggcc taggccgatc gagggcccag
     2581 gcaggtcgag ggcctggaag gaacaacccg actcggaaca aatctcccag tcggcaatct
     2641 ccaggttggc aatctccaag tcagaaatct ccaagtcaga acgggctaac tcaggacaaa
     2701 agctgatccg gatcgactaa tccggttaga atctggcgct ccggacatga cgagtcgagc
     2761 tcgtgagagc ctctcgtaaa cggactcctc gcaggaagaa gaaaaaggga aggtatcgtt
     2821 cgatgttttc ggcactctgg catcagtata cgcagggcgg ttgggccatg tggctcatca
     2881 tgttttggct cgtctgttcg atcgccgtga tcgtggagcg gacggtgttc ttgttcggcg
     2941 cttcgatcaa caaagacgtg ttcgtcgcca ccatgcagaa atgcatcctg gcgggcgacg
     3001 tggccaaggc cgtcaagatg tgctcggctg ccaacgctcc gctggctcgt atcgttcagg
     3061 cgggtctcgt caaggtcaac cgccccgacg aggaagttca ggcgggcatg gacgaggcgg
     3121 cgctgcgcga gatgccgacc atcaaccgtc g
//
LOCUS       DASTKU010000738         9726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1483846, whole genome shotgun sequence.
ACCESSION   DASTKU010000738 DASTKU010000000
VERSION     DASTKU010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483846"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VFQ61_25905"
     CDS             complement(<1..730)
                     /locus_tag="VFQ61_25905"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HET9957969.1"
                     /translation="MIERRATRAVILAAGTGSRLSDGRQELPKPLRKVAGVPMCVRVL
                     RTLAAAGLREAVVITGYQGELVRTALLSEPNLGLRLMFVDNPRYENKNGVSLLAARDF
                     INEECVLTMSDHLYSPELVRRLLAFELPEGACALGVDYDIERCFDLDDATKVGAERTR
                     ITAIGKELERYQALDTGVFRIGPALIEELSRLDAERGDCSLSDGVAALARRGQFFAAD
                     VGDVRWIDVDTPAALERAEAMLHVF"
     gene            complement(1007..1795)
                     /locus_tag="VFQ61_25910"
     CDS             complement(1007..1795)
                     /locus_tag="VFQ61_25910"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HET9957970.1"
                     /translation="MPNRNSGDSPTHAAVILVAGIGERLRPLTEDRPKALMDLGGETI
                     LGRALRQLYAAGVRDFVLATGYREEAIRAAWGELAPGLASARITYCPNPDFRTTQNSV
                     SLYLCERALEGKSFFKLDGDVVFQPEVLARLQATLEDTECDLAVACDSRRALDEEAMK
                     VQSQLGRIHRFGKRLPLSEAGGETLGIELVSASACQRLFETIGRAVRAGHLDRYYEHA
                     YSELCEDPQFDCRAVEVGDLPWSEVDTLADLEQARSVVRPGGSA"
     gene            1821..3134
                     /locus_tag="VFQ61_25915"
     CDS             1821..3134
                     /locus_tag="VFQ61_25915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HET9957971.1"
                     /translation="MQRPQKIEAAEGKLGVLLPGLGAVSTTFIAGCLLARRGLSRPVG
                     SLTQTGTIRLGKRTDSRNPKIQEFAPLAKLDDLCFGGWDLYPDTAYEAAVKAEVLESR
                     HLDPVREELSAIRPMSAVFYPEYVKRLHGSNVKKHANKAEHVEAIREDVRRFVKENGC
                     ARAVAVWCGSTEIYLEPRAVHQSIQSFERGLLDNDPAISNSMIYAWALLKEGVPFANG
                     APNLTVDIPAMHDLARETQTPVAGKDFKTGQTLMKTILAPGFKARMLGMKGWFSTNIL
                     GNRDGEVLDDPDNFKSKEVSKLGVLEQVLQPEVYPELYADLYHKVRINYYPPRGDAKE
                     GWDNIDIFGWLGYPMQIKVDFLCRDSILAAPLVLDLALFMDLSRRAGFRGTQEWLSFY
                     FKTPMTAPGLYPEHDLFIQSMKLKNTLRWMMGEELITHLGHEYYD"
     gene            complement(3167..4141)
                     /locus_tag="VFQ61_25920"
     CDS             complement(3167..4141)
                     /locus_tag="VFQ61_25920"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HET9957972.1"
                     /translation="MVSAFASRVAAFFLRPFAAWERFLFEECDALPLAVLRIALGAYL
                     LFYFAALYPLIELHFFPPALLGAQDWAGFPAPLLIRYVGSGLAPRLALYGLTLLACVA
                     LMLGVQTRLAASLLWLLDRSWLSFNAGHNSGDNVLLISTFLMAVAALFGHPQRVLSLD
                     AWFRTRETGRSPRPRVPAWPLRSFQVQLVLIYFFTGVHKLSNPDWYNGEALFYVFQQP
                     VWSRFDLTWLTGPVPIASITYGTLLFEVLLFPALVWVPAARAYVLLAGVLFHLGILLT
                     MRVFVFSEAMVLSYLCFVNSRPALVSIARSVLRRMPRHRIARERARRA"
     gene            complement(4135..4704)
                     /locus_tag="VFQ61_25925"
     CDS             complement(4135..4704)
                     /locus_tag="VFQ61_25925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957973.1"
                     /translation="MADGNTRVDPSSPTPGLARKAERQAPVHRRRLERWLRGLAAAGF
                     ALHFMALALNSLPNSRFIATLYPYYGFYPSHTGQNQAWMMYAFPDRHATEYELQARFP
                     DGHWEHPWGTSHQMSARKVYFLEATFGATRGEAGRFFEALRARYPEQRRPIEIRLRQT
                     SRPLNAFSDVPARGIFAGEEQVRDLNRIW"
     gene            complement(4782..7703)
                     /locus_tag="VFQ61_25930"
     CDS             complement(4782..7703)
                     /locus_tag="VFQ61_25930"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET9957974.1"
                     /translation="MAWVALSVGLYQCRDVEPPEASPDAAWLRINEVVSNNEGVWVDE
                     SGQTDDYIELLNTGSRPAPLKGLVFEDGGGRYPLPDMSVAPGQTLLLWADDDLKQGPN
                     HLAFKISSKGEPLRLLRPDGSAIDSVLVPALAEHQAYVRMPDGSGSPSVCDWATPGRS
                     NGARCGPSAPSSHLEHVEFAPYAWPETWPEPAAPLVITRLALRPAEFVEVTNTSTEPV
                     VLADYQLRLAPHAPGLVLPDALAGTSVAWPKQVLEAGQSITVPVTDEDVASIQASAHF
                     QGLASLFSTQSAGALVDRLEFSDWPEGAKLERDAGERQAPRFCEASTAEAGAEPCRPL
                     PKREVGSYMNRLLTPGDFEALARGRSDLGIDSVEFLVDMSAGDSVTFLNSRDWDLHYS
                     FVREVIQHQFHLNRCIPEEKAQFEQGWQEFSQQEYFRVEGRRYLLGTLVRYASNQLLS
                     VEFSPGDVISAEQMKRAFFAVMKRVPDARAYSIRPQAPDQIQRLRQVEGQVPIVGPSA
                     AFEGVTFQALAPGLSFGALRFVPADRLREEGVGPRDIIITDQVPNDLPLVGGLITEVF
                     QTPLSHVNVLSRGRGTPNAALPNARQDPRVQPLLGELVRFEVHGGTFSIEKADPLEAS
                     RFFEMQKPKERLVPRLDLSVRGLASLDQRSFEDLPAIGGKAAQFAELSRVSWCGTAGA
                     PENAFAIPVGYSIDHFDRSGARAELQRLREQPGFASDPRERAAGLAHVRELIESTPLD
                     PELDALLQSAIAARLTGKSLRFRSSSNVEDLAGFNGAGLYTSVGVDDGDLEDGVARAV
                     RRVWASLWNDRAYAERDFYNVDQDAVGMGVLVHEGFPSERANGVAISRNVLEPTRADV
                     YYVNAQLGEALVTNPAPGIQSDEFTYSRFGYEKFVYQQRSTFSPLRPVLSEEEVSLLA
                     CNLSAIHESFRSFLDPERKNPAFAVDIEFKFVGAERRLVIKQARSFALGKSVPDSWCD
                     F"
     gene            7795..8427
                     /locus_tag="VFQ61_25935"
     CDS             7795..8427
                     /locus_tag="VFQ61_25935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9957975.1"
                     /translation="MILIGQYDSPFVRRVAIALTLYEIPFEQNPWSVWTNAEEIAQYN
                     PLRRVPTLVLPDGEWLIESFAILETLDELVGPERALLPAHGPVRRDGLQVCALATGVA
                     EKAVSLLYELLLREVPSPLWCTRCGRQISDALTLLNRDRAQRTTPYWLGEQLSHADIA
                     VACALRFVREAHAELFESLKIPALRQHSERCESLPVFQTVSQPLTVTLAR"
     gene            complement(8486..>9726)
                     /locus_tag="VFQ61_25940"
     CDS             complement(8486..>9726)
                     /locus_tag="VFQ61_25940"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HET9957976.1"
                     /translation="PTYDVRSLRMWLKSNESVDLVAFFILRTRHSDPMTTDDNDELAL
                     IPFPVHALFTEHLPSFDAVILQDIDAVTYELSQYLPALESYVRSGGGLIMVGGPSSFA
                     GGGYARSALERVLPTSLGDPRQPYDLAEFLPRYTEAGRVAPVLAALRDHVSEDLPRML
                     GTNLLGPAREGAIVLWEHPTKSVNGRAMPVLALGDAGDGRSIALSVDGTHQLAFSEFA
                     ERAAGRAYGALWDGLLGWLMRDPRYEAVRMELAEPCMAGEKTKLRLTRLPGAPGELEL
                     SIEALGEARQVVQTQRATVPSSGVVELELSPLAAGGYAALAKAGQAPPTRFDFACERG
                     GSAWGDVRPDPDRLARIARASNGVSVDAAGASKIPLPEPAVVSAQREVSPVLPAWVWA
                     LIASVALGAHWIARRRLGLA"
BASE COUNT         1913 a         3135 c         2962 g         1716 t
ORIGIN      
        1 cgaaaacgtg cagcatggct tctgcgcgtt ccagagccgc gggcgtgtcg acatcgatcc
       61 aacgcacgtc gccgacgtcg gccgcgaaga attgcccgcg acgcgccaga gccgccacgc
      121 catcgctcag cgagcagtcg ccgcgctccg cgtcgagccg cgagagctcc tcgatcaacg
      181 cggggccgat acgaaacaca ccggtgtcga gggcctgata acgctccagc tccttgccga
      241 tcgcggtaat ccgcgtgcgc tctgcgccca ccttcgtcgc gtcgtccaag tcgaagcaac
      301 gctcgatgtc gtagtccact ccgagcgcgc aagcgccctc tggcagctcg aacgccaaca
      361 ggcgccgcac tagctccgga gaatacaggt ggtccgacat cgtgagcacg cactcttcgt
      421 tgatgaaatc gcgcgccgcg agcagcgaga ccccgttctt gttctcataa cgcgggttgt
      481 ccacgaacat caggcgcagc ccgagattcg gctcgcttag cagcgccgtg cgaaccagct
      541 ccccttggta tccggtgatt actacggctt cgcgaagccc agcggccgca agagtacgga
      601 gcacccgaac gcacatcggc acccctgcca ccttacgcag aggtttgggc agctcttgcc
      661 tgccatcgct gagtcgggag cccgtcccag cggcaaggat cacggcccgg gtggcgcgtc
      721 gttcaatcat ctcaaggtct cgctttcgtc acttcgcgtt aagcaacgcc cgagccatta
      781 gcacgtggcg tgacaacggg gcaaatcctt ttccaatcgc gtacttagct gcactttgga
      841 cctgtgatgg gggtaccaca ctgtggtgga tttgcgtcat aaccgtcgct tctcggtgtc
      901 gcgtgctccg ggagctacac ccttagaatc cttagagatg ggcgcccgtt tcacaaatac
      961 tgtgttgata tggcgtcagt gaatccaggt tgggaagtgt aaattcttac gcacttccgc
     1021 ctggccgaac gacgctacgc gcctgctcca agtcggccag cgtgtccacc tcgctccacg
     1081 gcaaatctcc cacctcgacc gcgcggcaat cgaactgagg atcctcacac agctcggaat
     1141 aggcgtgttc gtagtagcga tctaggtgac ccgctcgtac ggcgcggcca atcgtctcga
     1201 acaatcgctg acaagcgctt gcggaaacga gctcgatgcc caaagtctcc ccaccggctt
     1261 cgctcagcgg cagtcgcttt ccaaagcgat gaatccggcc gagctgcgac tgtactttca
     1321 tggcctcttc atcgagcgct cgtcgtgaat cgcaagccac agccaggtcg cactcggtat
     1381 cttccagggt ggcttggagc cgcgctagaa cctcgggctg aaatacgacg tcgccatcca
     1441 gcttgaagaa gctcttcccc tccagagctc gctcgcacaa gtacaacgag acgctgtttt
     1501 gagtcgtgcg aaaatcgggg tttgggcaat aggtgatgcg cgcggacgcg aggcccggcg
     1561 cgagctcccc ccacgcagca cgaatcgcct cttcgcgata acccgttgcg agcacgaagt
     1621 cgcgcacgcc ggccgcataa agctggcgca aagcgcggcc gaggatcgtc tcgcccccca
     1681 aatccatcag agctttcggt cgatcctcgg tcagtggtcg caaacgctca ccaattcccg
     1741 ccacgagaat tacggctgca tgcgtcggtg aatctcccga gttgcgattg ggcatggcgc
     1801 ttgctaccac ggccgaagcc atgcaacgac cccaaaagat cgaagctgcc gaaggcaagc
     1861 tcggcgtttt gctgcccggc ctaggtgccg tttccacgac gttcattgcc ggctgcctgc
     1921 tcgcgcggcg cgggctttcc cgcccggtcg gctcactcac gcagaccggt acgatccgac
     1981 tcggcaagcg cacggattcg cgcaatccca agattcagga attcgcgccg ctcgcgaagc
     2041 tcgatgacct gtgttttggc ggctgggatt tgtatccgga tacggcctat gaagcggccg
     2101 taaaggccga ggttctcgag agccgtcacc tggatcccgt gcgcgaagag ctgagtgcga
     2161 ttcgcccgat gagcgccgtg ttctacccgg agtacgtcaa gcggttgcac gggagcaacg
     2221 tgaagaagca cgccaacaag gccgagcacg tggaagcgat tcgcgaagac gtgcgacgct
     2281 tcgtcaaaga gaacggctgc gcacgcgccg tcgcggtttg gtgcggatcg accgaaattt
     2341 acctcgagcc gcgcgccgtt catcagagca tccagagctt cgaacgaggc ctgttggaca
     2401 acgacccagc gatctcgaac tcgatgatct acgcttgggc actcttgaag gagggcgtgc
     2461 cgttcgccaa cggagccccg aacctgaccg tcgacatccc cgcgatgcac gatctcgcac
     2521 gcgagacgca gacgcccgtc gccggtaagg acttcaagac ggggcagacc ctgatgaaga
     2581 cgattctggc gcccgggttc aaggctcgca tgctcggaat gaagggctgg ttcagcacca
     2641 acatcctcgg caatcgggac ggcgaggtgt tggatgatcc ggataacttc aagtcgaagg
     2701 aagtaagcaa gctcggcgtg ctggagcagg tgctgcagcc cgaggtttat ccggagctgt
     2761 acgcggatct ctaccacaag gtccgaatca actattaccc cccgcgcggc gacgccaaag
     2821 agggctggga caatatcgat atcttcgggt ggctcggcta cccaatgcaa atcaaggtcg
     2881 acttcttgtg ccgtgactcg atcctggccg cgcccttggt gctcgacctc gcgctgttca
     2941 tggatctctc gcgtcgcgcg ggcttccggg gcacgcaaga gtggctgagc ttctacttca
     3001 agacgccgat gaccgcgccg ggcctctacc ccgaacacga cctgttcatc cagtcgatga
     3061 agctgaagaa caccttgcgc tggatgatgg gtgaagaact catcactcac ctcggtcacg
     3121 aatactacga ctgatcgacc tgacttttcg caacaggctc ggaggttcaa gccctccgag
     3181 cccgctcccg ggcgatgcgg tggcggggca ttcgccgaag caccgagcgc gcaatcgaaa
     3241 ccagcgcggg gcggctgttc acgaagcaga gatagctcaa gaccatggct tcgctgaaca
     3301 cgaagactcg catggtgagc aaaatgccca agtggaacag cacgcccgct agtaaaacat
     3361 aggcgcgcgc cgcggggacc cagaccaagg cggggaacaa cagcacctcg aacaacagcg
     3421 ttccgtaggt gatcgaggcg atcgggacgg ggcccgtgag ccacgtcaga tcgaagcgcg
     3481 accagacggg ttgttgaaac acgtagaaca gtgcttcgcc attgtaccaa tccgggttcg
     3541 agagtttgtg gaccccggtg aagaagtaga tgagcacgag ctgcacttgg aacgagcgca
     3601 ggggccatgc cggaacacgc gggcgcggtg aacgcccagt ttctcgcgtt cgaaaccagg
     3661 cgtccaacga gagcacgcgc tgcgggtgcc cgaacagcgc cgcgaccgcc atcaagaacg
     3721 tcgagatgag cagcacattg tcgccgctgt tgtgccctgc gttgaagctg agccaggagc
     3781 gatccaggag ccagagcagc gaggccgcca gtcgggtttg aactccgagc atcagtgcga
     3841 cgcaagccag caaggtcaaa ccgtacagcg cgaggcgtgg cgcgagaccc gagcccacgt
     3901 agcgaatcaa cagcggcgcc gggaaccccg cccaatcttg cgcaccgagc agcgcgggcg
     3961 ggaagaagtg gagctcgatc aacgggtaga gagctgcgaa gtaaaacagg agataggctc
     4021 cgagcgcgat gcgcagcacg gctaacggca gcgcatcaca ctcctcgaac aagaaccgct
     4081 cccaagccgc gaacggccgc aggaagaaag ccgcgacgcg cgacgcaaag gcgctcacca
     4141 tatacgattc aggtctctga cctgctcttc gccagcgaaa atcccgcgcg caggcacgtc
     4201 cgaaaatgcg ttcaaggggc gactggtttg gcgcagtctg atttcgatcg gacgtcgctg
     4261 ctccgggtag cgcgcgcgca aggcctcgaa gaagcgcccc gcttcaccac gggtagcccc
     4321 gaaggtcgct tccaggaaat agaccttacg cgcgctcatc tggtgcgacg ttccccaggg
     4381 gtgttcccag tggccgtctg ggaagcgggc ttgtagctcg tactcggtcg cgtgacgatc
     4441 agggaaagcg tacatcatcc aggcttgatt ctgcccagtg tggctcgggt agaacccgta
     4501 gtacggatag agcgtcgcga tgaagcgcga gttcggcagt gagttcaagg ccagagccat
     4561 gaagtggaga gcgaagcccg cggccgccaa gccacggagc cagcgctcga gacgccgacg
     4621 atgaaccggc gcttgccgct ctgccttccg tgccaatccg ggggtcggcg aggatggatc
     4681 aacgcgagta ttgccgtcag ccatctatac ggcgccgtag gttgctcggg tctgacgact
     4741 tttgcaacgc agacgaggcc tcgagtcccg cgttcgtgac gtcagaagtc gcaccacgaa
     4801 tcgggtacac tcttacccaa cgcgaacgag cgcgcctgct tgatcacgag acgccgctct
     4861 gcccctacga acttgaattc gatatccacc gcaaaagccg gattcttgcg ctcggggtcc
     4921 agaaacgacc taaaactctc gtgaatcgcc gacagattgc aggctaggag cgaaacttcc
     4981 tcttcactca acacggggcg caaaggactg aacgtgctgc gctgttggta gacgaacttc
     5041 tcgtatccga accgtgaata agtgaactcg tcactctgaa ttccgggagc agggttggtc
     5101 acgagggcct cgccaagctg cgcgttcacg tagtaaacat cggcgcgcgt cggctcgagc
     5161 acgtttcgac tgatggcgac accgttggct cgttccgacg ggaagccctc gtgcaccagc
     5221 acgcccatgc ccaccgcgtc ctgatcgacg ttgtagaagt ctcgctccgc gtaggcgcgg
     5281 tcgttccaca ggctcgccca cacgcgccgc acggctcgag ccactccgtc ctccaaatcg
     5341 ccgtcatcca cgccgacgga ggtgtatagc cctgcaccgt tgaagcccgc caagtcctcg
     5401 acgttgctgc tactccggaa acgaagagat tttcccgtca ggcgcgccgc gatcgcagac
     5461 tgcaacaagg cgtcgagctc cgggtccaat ggggtcgact cgatcaactc tcgtacatgg
     5521 gctagaccgg cggcacgctc ccgcggatcg gaagcgaagc ccggttgttc gcgcagtcgc
     5581 tgaagctcgg cgcgagcgcc ggagcgatcg aagtgatcaa tcgaatatcc gacggggatc
     5641 gcaaacgcat tctcgggagc gccggctgtg ccgcaccaac tcacgcgcga gagttcggcg
     5701 aactgtgccg ccttaccccc gatcgcgggc aagtcctcga agctgcgttg gtctagcgag
     5761 gcgagccctc gcacggagag atcgagccgt ggaacgagcc gttccttcgg cttctgcatt
     5821 tcgaagaagc ggctcgcctc cagagggtcc gccttttcta tcgagaatgt gccgccatgc
     5881 acttcgaaac gtacgagctc ccccaacagc ggctgcaccc ggggatcctg gcgtgcattc
     5941 ggcagcgccg cattcggcgt accccgcccg cggctcagaa cgttgacatg tgatagcgga
     6001 gtttggaaca cctcggtgat cagcccgccc accaacggca agtcattggg aacttggtcg
     6061 gtaatgatga tgtcgcgtgg ccccacccct tcttctcgta gccggtcggc aggtacgaat
     6121 cgcagcgctc cgaagctgag cccgggggcc aaagcttgga aggtgacgcc ttcgaacgca
     6181 gcgctcggcc caactatggg cacctgcccc tccacctggc gcaaacgttg aatctggtca
     6241 ggagcctgag gacgaatgct gtaagctcga gcgtcgggaa cgcgtttcat caccgcgaaa
     6301 aacgcgcgct tcatctgctc ggcgctaatc acgtcacccg gagagaactc caccgagagc
     6361 agttggttgc tcgcataccg caccaaggtg ccgagcagat atctcctccc ctcgacgcgg
     6421 aagtactcct gctgactgaa ctcctgccag ccctgctcga attgggcctt ctcttctggg
     6481 atgcagcggt tcaaatggaa ctggtgttgg atgacctcgc ggacgaagct gtagtgcagg
     6541 tcccagtcgc gtgagttcag gaacgtcacg ctgtctccgg cgctcatgtc caccaggaac
     6601 tcgaccgagt cgatcccgag atcgctgcga ccgcgcgcca gagcctcgaa atctccgggc
     6661 gtgagcaggc ggttcatgta gctgccgact tcacgctttg ggagtggacg acaaggctcg
     6721 gcccccgcct ctgccgtcga cgcttcgcaa aagcgcggcg cctggcgctc tccggcgtct
     6781 cgctcgagct tggcgccctc cggccagtcg ctgaactcta ggcggtcgac cagagcgcct
     6841 gccgactggg ttgagaatag gctcgcgagc ccctgaaagt gcgcgctcgc ttggatgctg
     6901 gccacgtctt catccgtgac cggcaccgtt atcgactgcc ccgcctcgag cacctgcttc
     6961 ggccaagcca cactcgttcc cgccagcgcg tcgggcaaca cgaggcctgg agcatggggc
     7021 gccaagcgca gctggtagtc ggccaggacg actggttcgg tcgaagtgtt ggtgacctcg
     7081 acgaactctg ctgggcgcaa cgcgagtcga gtaatcacca gcggcgccgc gggctcgggc
     7141 caggtctccg gccaagcata aggagcgaac tccacgtgtt cgaggtggct ggacggcgcg
     7201 ctcggcccgc accgcgcacc attgctccgc ccgggtgtgg cccagtcaca cacgctcggg
     7261 gaaccggagc catccggcat tctgacgtag gcctgatgct ccgcgagagc tgggacgagc
     7321 acggaatcga tggcggagcc gtcggggcgc aatagacgaa ggggctcacc tttcgaggag
     7381 atcttgaaag ccaggtggtt tggcccctgc ttcagatcat cgtccgccca cagcaacagg
     7441 gtttgaccgg gcgcgacgct catgtccggt aagggatatc ggcctccccc atcctcgaat
     7501 accaggccct tcagcggtgc tggacgcgag ccggtattta ggagttcgat gtagtcatcc
     7561 gtttggccgc tctcgtccac ccagacgcct tcattgttcg agactacctc gttgatgcgc
     7621 aaccaagccg cgtcggggct cgcttcgggg ggctccacat cacggcactg atacagcccc
     7681 acgctcaacg cgacccacgc catccggctc cgaaccaacc gcttcacagg gcagcagtgt
     7741 agctcgtttt ccgtgttggt tgaggcgttc gtgaactgag actaacttta gcgcgtgatc
     7801 ctcatcgggc agtacgactc gcctttcgtg cggcgggtcg caatcgcgtt gaccctgtat
     7861 gaaatcccct tcgagcaaaa cccctggtcc gtttggacga acgccgaaga gattgctcag
     7921 tacaaccccc tgcgtcgggt gcccacgttg gtccttccag atggggagtg gttgatcgag
     7981 agcttcgcaa tcctggaaac gttggacgag ctcgtcggcc cagagcgggc cttgttgccg
     8041 gcgcacggcc ccgtacgccg cgacggcttg caggtttgcg cactggcaac cggcgtcgcg
     8101 gagaaggcgg tcagtctgct gtacgagttg ctgctgcgcg aggtcccctc accgctgtgg
     8161 tgcacgcgct gcggtcggca gattagcgat gcgctcaccc tgctaaaccg agatcgagcg
     8221 cagcgcacga cgccttattg gctcggcgag cagctgagtc acgccgacat cgccgttgcg
     8281 tgtgcgcttc gcttcgtgcg tgaggcgcac gcggagctgt tcgagagcct gaagattccg
     8341 gccttgcgcc agcactcgga gcgatgtgaa agcctgccgg tgttccagac cgtttctcag
     8401 ccgctcacgg taaccctcgc gcgttgaggc cctcatcgtc gaatccactc cgcaaacctg
     8461 tcctggaatg ggcgagcaaa cgctttcagg caaggcccag tcgccgacgg gctatccaat
     8521 gtgcacccag cgcaaccgag gcgatcaggg cccagaccca ggccggcaaa accggggata
     8581 cttcgcgttg cgcgctcacc accgcgggct ccggcaatgg gatcttcgac gcgcccgccg
     8641 cgtccacact cactccgttg ctcgctcgtg caatgcgcgc cagtcgatcc ggatcggggc
     8701 gtacgtcccc ccaagcgctt ccacctcgct cgcacgcaaa atcgaagcgc gtcgggggcg
     8761 cctgaccggc cttggccagc gccgcatacc ctcccgctgc cagcggtgaa agctccagct
     8821 cgaccacgcc ggagctcgga accgttgcgc gttgcgtttg caccacttgg cgggcttcgc
     8881 ccaaggcctc gatgctcaac tcgagctcac cgggcgcgcc gggcagacgg gtgaggcgga
     8941 gcttggtctt ttctcccgcc atgcaaggtt cggcgagctc catgcgcacc gcttcgtagc
     9001 gcggatcgcg catcaaccac cccagcaggc cgtcccatag cgccccgtag gctcgacctg
     9061 ctgcgcgctc ggcaaactcg ctgaacgcga gttggtgagt gccgtcgacg gacagcgcga
     9121 tgctgcgccc atcgcctgcg tctcccaatg ccaagaccgg catcgcgcgc ccattgacgc
     9181 tcttggtcgg gtgctcccac agcacgatcg ctccctcgcg cgcgggcccg agcaaattgg
     9241 tgcccaacat gcgaggcaaa tcctcactga cgtggtcgcg cagggccgcg agcaccggtg
     9301 ccactcgacc ggcctccgtg tagcgcggca aaaactccgc cagatcgtag ggctggcgtg
     9361 gatcgccgag gctggtgggc aacacccgtt cgagcgcgga gcgtgcatag ccaccacccg
     9421 caaaggacga aggcccgccc accatgatga gtcccccgcc cgagcggacg tagctctcga
     9481 gcgcaggtag gtattgcgac agctcgtacg tgaccgcgtc gatgtcctgc aaaatcacgg
     9541 catcgaagct gggtaagtgc tcggtgaaca gcgcgtgcac tgggaatggg attaacgcga
     9601 gttcgtcgtt gtcatcggtg gtcatcggat cgctgtgccg cgtgcgcaag atgaagaacg
     9661 caacgagatc gacggactca ttgctcttga gccacatcct gagtgaacgc acgtcgtaag
     9721 tggggc
//
LOCUS       DASTKU010000739         4983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_367065, whole genome shotgun sequence.
ACCESSION   DASTKU010000739 DASTKU010000000
VERSION     DASTKU010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367065"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFQ61_25945"
     CDS             complement(<1..451)
                     /locus_tag="VFQ61_25945"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET9957977.1"
                     /translation="MSSLPRISTAAKVGAFAIVTMVLAIFIYRYASKMGGAGSGYRVY
                     ALMNDATGVAQRSQVRVAGIPVGVVESVKLQGAKARVDIRLEPEIALYEDAVVAKVSS
                     SLLGEYFLSLSAGTEGRRKLKDGDRIGVVVEAATTDQIMKDVSSITKD"
     gene            697..2205
                     /locus_tag="VFQ61_25950"
     CDS             697..2205
                     /locus_tag="VFQ61_25950"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET9957978.1"
                     /translation="MQSCEHSLARPEPRNASPLNVFPLDASPLQGFSDRASSPASSSA
                     ARSAEVTFRIAVMDSSMRTLRCSSLGFKADVCMFGRRTEAGLHEKSCARAPCGKPRAG
                     WRDVQGSLSKAALSLGYIALVGCSDASLSRNTQSPNHAITSTPAASDYSRSPEPDPKP
                     ELPLALECPNRDAALRRVAAIKANEVARGANIPDASEIERALRSEGAPYMWPYAFTLE
                     GDVTVADQAREPLRRFLTSFHPQGQQRCGVAFEQTPDGRGVVLAISVDALADLEPLPK
                     QVRVGSWVEVNANVIVPATDAKFVVLGPRGAPKGVPTSFQLSHAQARFSADAEGLWLV
                     QLVAETGSGPRPVLEAELIAGSPASLPTLDTSCADPNPVPPEVAPLLLFDKLSSLRKS
                     EKLEPLERVPELDALAQKQAVALLRAQRLAHDLGSGDPEARVLAAGLDAEVVGENVAH
                     ARDVGRSHCALYRSPSHRANMLSPHVNSVGLGVARDPDGSIWVCQLFARLHR"
     gene            2553..4202
                     /locus_tag="VFQ61_25955"
     CDS             2553..4202
                     /locus_tag="VFQ61_25955"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9957979.1"
                     /translation="MSSIASAQAGYFYLDRAQLSGAPDDGFTVFRPYVPRKNRFYATA
                     AFGYTLNPLRSETVTRNSKIEEQIDNPMQGQLIAYASAGVQVLSRVGVSLTLPILLRG
                     FPGDDPQVYGVGAGGIGDVNTAVHDLRIDLRVRTYETDDGVARFGGGVAMFAPTGNGA
                     AFASDDQVTGWVFGSAEFDLGDLLLAGHIGPHFRPERSIGGQYGGLYLASEMRFAFGA
                     YVPLRQGRIRLGGELFGGTGLESGLGPRNANTIFSQRNTDVEWLGQARFLIERDQQIY
                     VNAGFGTRLSTGYGAPDMRVLASIGRYFEWFDSNANSPKPKRRVQPTAAHYDLDTDKD
                     GFPDAIDACPTIKEDGKEPDPTDGCPGTSDRDGDGIPDSTDVCPDKPEDKDGIQDDDG
                     CPEEDADNDTIPDVQDKCPLEPGTRSADPERFGCPTLTRLDDTGSIVLLKPIEFEFGR
                     AVIKAESFPILDEVLTLMKARPELRVGVYGHTDNRGGDALNLRLSKERAAACVTYLTE
                     HGISADRLESEGFGRTQPVADNATDEGRARNRRVDFKVLSE"
     gene            complement(4505..4672)
                     /gene="rpmG"
                     /locus_tag="VFQ61_25960"
     CDS             complement(4505..4672)
                     /gene="rpmG"
                     /locus_tag="VFQ61_25960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010364212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET9957980.1"
                     /translation="MRENIRLVSSAGTGFTYYTTKNKRTQTAKLELKKYDPVARKHVP
                     FKEAKMPPHSK"
     gene            4890..>4983
                     /gene="rpmH"
                     /locus_tag="VFQ61_25965"
     CDS             4890..>4983
                     /gene="rpmH"
                     /locus_tag="VFQ61_25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET9957981.1"
                     /translation="MKRTYQPHNTSRLRTHGFRARISTRGGRKVL"
BASE COUNT          892 a         1593 c         1620 g          878 t
ORIGIN      
        1 cgtccttcgt gatcgaagac acgtccttca tgatttgatc ggtcgtggcg gcttccacca
       61 ccacgccgat gcggtcgccg tccttgagtt tgcgcctgcc ttcggttcct gcgctgagag
      121 aaagaaagta ctcgccgagc aagctcgagc tgaccttcgc gaccacggcg tcttcataga
      181 gtgcgatttc gggctcgagt cggatgtcga ctctcgcctt ggcgccctgg agtttcacgc
      241 tctcgacgac gcccaccggg ataccggcga cccgcacctg agatcgctgc gcaacgcccg
      301 ttgcgtcgtt catcagagcg taaacgcgat acccagatcc cgcgcccccc atcttgctgg
      361 cgtaacggta gatgaagatc gccagtacca tcgtaacgat ggcgaacgct ccgaccttgg
      421 cagccgtgga gatgcggggc agactactca tcgcatctct cgggtactgg gctcaacgaa
      481 gcgaggcccg ccgcgccgtg ggctccacga cgcgaggctc gccgagctgg attgcgcgct
      541 ggcctccaac cgagaagctc gactatcctg cagagtggtg tgaagcaaag tcactggaac
      601 ccgagtagta agcgactgca gggatagaac aagcgtcgga gcgacgaaag aaagcagccg
      661 gcgtttgtcg gcggcgactt taggtgagga gtcaagatgc agtcctgcga gcatagcctg
      721 gctcggcccg aaccccgcaa tgcttctccg ctcaatgttt ttccgctgga tgcctctccg
      781 ctgcaaggct tctccgatcg cgcgtcctcg ccggcctcga gttcggccgc tcgatcggct
      841 gaggtgacct tccgcatcgc cgtcatggat agctcaatgc gcacgctccg ctgcagtagc
      901 cttggtttca aggcagacgt ttgcatgttc ggacgcagga ctgaggcagg gctgcacgag
      961 aaatcgtgtg caagggcgcc gtgcggcaag ccgagggcgg gatggcgcga cgtccaagga
     1021 tcgctgtcga aggcagcgct tagcttgggt tacatcgcgc tggtgggctg cagcgatgct
     1081 tcgctgagcc ggaacacaca atcgccgaat cacgcgatca cgagcacacc ggccgccagt
     1141 gactactccc gctcgcccga gcccgatccc aagccggagt tgcccctggc cttggagtgc
     1201 cccaatcgcg acgcggcgct gcgtcgggtc gcggccatca aggcgaacga agtcgcgcgg
     1261 ggagcaaaca tcccggatgc ctcggagatc gaacgcgccc tccgatcgga gggtgccccc
     1321 tacatgtggc cctacgcttt caccttggaa ggtgacgtga cggtcgccga ccaagcccgc
     1381 gagccgttgc gtcgattctt gacctcgttt catccgcaag ggcagcaacg ctgcggcgtc
     1441 gccttcgaac agacgccaga cggacgtggg gtggtgctcg ccatttccgt cgacgccttg
     1501 gcagacctcg aacccttacc gaaacaagta cgggtgggca gttgggtcga ggtgaatgcc
     1561 aacgtcatcg tgcccgccac cgacgccaag ttcgtggtgc tcgggccacg tggagcaccc
     1621 aagggtgtgc cgacttcctt ccagctgtcc cacgcccaag cacgattttc ggctgatgcc
     1681 gaggggctgt ggctcgtcca gttggtagcg gagacgggtt ccggtccgcg cccggtgctg
     1741 gaggccgagc tcatcgcggg gagccccgcg tccctaccca cgttggacac gtcctgcgcc
     1801 gacccaaatc cagttccacc cgaagtggct ccgctactgc tgttcgataa gctgagttct
     1861 ttgcgaaaga gcgagaagct cgagccgctg gaacgggtcc cagagctgga cgcactcgcc
     1921 cagaaacaag cggtggctct actgagggcc cagcggctcg ctcacgatct aggctcaggc
     1981 gatcccgagg ctcgggttct tgccgcggga ctggacgcgg aggtcgtcgg ggagaatgtg
     2041 gctcacgctc gtgacgtcgg ccgcagccat tgcgctctgt accgaagccc ctcgcaccga
     2101 gccaacatgc tgagcccaca cgtcaactcg gtagggctgg gggtggcccg tgatccggac
     2161 ggcagtatct gggtctgcca gttgttcgcc cgcctccacc gctgatcccg gaacgcggcg
     2221 cggcccgagc acgagcaact cccggcgtga cccgcgtgcc cgaatcctag aggacactca
     2281 cccgtcatgg gggcgtttac ccacgtgaga gagagcggga aattggtgtt caattgtggg
     2341 ccccaccgag ctacgttggc cgcgtgcaac gttcggacat cattcgaaga gttatggggc
     2401 gcggctcgct gctgacgaag ccagaccagc ctcgggtcaa aggggtagag tgccgtcgtc
     2461 aggcgggttc gggccaaatc ggggtgcgtc cggaacgtgc gcgaatcagg cgagcagctc
     2521 tgtgcgtctt tgcgatgatc gcgatgtgcc tcgtgtctag catcgcgagt gcgcaagccg
     2581 gttacttcta tctagatcgg gcacagctgt cgggtgcgcc cgatgacggc ttcaccgtgt
     2641 tccgcccgta cgtgccgcgc aagaatcgct tctacgccac ggccgcgttc ggttacacgc
     2701 tcaatccgct gcgctccgag accgtgacgc gcaactcgaa gatcgaggag cagatcgata
     2761 accccatgca ggggcagttg atcgcttacg cgagcgcggg cgtacaagtg ctctcgcgcg
     2821 tgggtgtgtc actcacctta cctatcctgc tgcgcggttt cccgggggac gacccgcaag
     2881 tttacggcgt gggcgcaggc ggcatcggcg acgtgaacac tgccgtgcac gacctgcgca
     2941 tcgatctgcg cgtgcgcacg tacgagaccg acgacggagt ggcgcgcttc ggtggcggcg
     3001 tggcgatgtt cgcgccgacc gggaacggtg cggccttcgc gtccgacgat caggtcaccg
     3061 gctgggtctt tggctccgcc gagttcgatc tcggagacct gcttttggcg ggacacattg
     3121 ggccccactt ccgcccggag cgcagcatcg gcggccagta cggtgggctc tacctcgcct
     3181 ccgagatgcg cttcgccttc ggtgcttacg tgccgctgcg ccaaggcagg attcggctcg
     3241 gtggcgagtt gttcggcggc acggggctag agagcggcct cgggccgcgc aacgccaaca
     3301 ccatcttctc tcaacgcaac acggacgtgg agtggctcgg acaagcgcgc tttttgattg
     3361 agcgcgacca acagatctac gtgaacgccg gcttcggcac acgcctgtcc actggctatg
     3421 gcgcgccgga catgcgcgtg ctcgccagca ttggacgcta cttcgagtgg ttcgattcca
     3481 acgccaactc acccaaaccg aagcgacgcg tgcagccgac ggcggcccac tacgatctgg
     3541 acacggacaa ggacggtttc ccagacgcga tcgatgcatg cccgaccatc aaagaggacg
     3601 gcaaggagcc cgatccgacc gatggttgcc cgggcacttc ggatcgtgac ggtgacggta
     3661 tccccgacag caccgacgtt tgcccggaca agccggaaga caaagacggg atccaagacg
     3721 acgacggctg cccggaagaa gacgccgaca acgacacgat ccctgacgtg caggataaat
     3781 gcccgctcga gcctggcacg cgcagcgcgg atcccgagcg cttcggctgc ccgacgctga
     3841 cgcgcctgga tgacaccggc tccatcgtct tgctcaagcc gatcgaattc gagttcgggc
     3901 gcgccgtgat caaggcggag agcttcccga ttttggacga ggtgctgacg ctgatgaaag
     3961 cccgccccga gctccgcgtg ggtgtctacg ggcacaccga caatcgcggc ggggacgcgc
     4021 tcaatctacg tttgagcaag gagcgcgcgg ccgcgtgcgt cacctatctc accgagcacg
     4081 gcatctccgc cgatcgcttg gagtccgaag ggttcggtcg gacgcaaccc gttgcggaca
     4141 acgcgacgga cgaagggcgg gcgcgcaacc gtcgcgtgga cttcaaggtg ctcagcgagt
     4201 gagagcgacg agcgcggaga ttggcggcct gtgtagccaa tctccgcgcg aaggcgcggt
     4261 ccgacgccgt cgcacgctcg acggcgcagc cgttcgcgaa tcaacggagg gcaccttgcg
     4321 tgggttgttc gagtggtggc caggcgcggc tgcatagacg ggcccgcacg gtgacgcggc
     4381 gcccgccacc gtgaacggga cgggcggcgc gcactttaca gcgcgggcct cagcagacgg
     4441 agcacaccct gcgtagcgag tgccgagcac tcacccgctc actcgtcagc taaacgcctg
     4501 tcactcactt ggagtgcgga ggcatcttgg cttctttgaa cggaacgtgc ttgcgggcga
     4561 ccgggtcgta cttcttcagc tcgagcttcg cggtctgcgt gcgcttgttc ttggtcgtgt
     4621 agtaggtgaa gccggtaccg gcggacgaaa cgaggcggat gttctcgcgc atggcggcgg
     4681 ggaattatca cggcccccca cacctcgcaa tatggagcat ccaggttgga gcgaaggccg
     4741 cgcctcggtc gaacccgagc tgcgggatct gaacgcgggt ccaggtggtg gtgtggcgca
     4801 tcctgcagaa ttgcggtctc agcccttgac tttgcgcggc tggcgtggcg atcttcgcga
     4861 ccccgaacga acttcggacc ggtggcccaa tgaagcgcac gtaccaacct cacaatacta
     4921 gccgtctccg cacgcacggt tttcgcgcgc gcatcagcac ccgtggtggc cgcaaggtcc
     4981 tcg
//
LOCUS       DASTKU010000740         3168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1261909, whole genome shotgun sequence.
ACCESSION   DASTKU010000740 DASTKU010000000
VERSION     DASTKU010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261909"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFQ61_25970"
     CDS             <1..330
                     /locus_tag="VFQ61_25970"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET9957982.1"
                     /translation="EFEAQALKSAGFELDVVPPRYRAAYFSHCFSDALGYSAGYYSYI
                     WSEVLARDTEAWFGAHGGLSRPAGDILRQQILAKGNSVDPSQAFQAFYGRAPDIEPLL
                     EKRGLLP"
     gene            578..913
                     /locus_tag="VFQ61_25975"
     CDS             578..913
                     /locus_tag="VFQ61_25975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957983.1"
                     /translation="MLQATWSGYIDQASCGHYPAELRLMLGGRRIRFAIFDALAVTGY
                     AANSHVPGGQLLAVCKDAGAERVIVITDSSAIRMIGSTVALATGLSIRFVATDGQAVS
                     WLREQGVEV"
     gene            complement(960..2816)
                     /gene="ggt"
                     /locus_tag="VFQ61_25980"
     CDS             complement(960..2816)
                     /gene="ggt"
                     /locus_tag="VFQ61_25980"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET9957984.1"
                     /translation="MAWLGSWLLVSSACSQARTEGVTAPAPFGSASPPSSAMLAERLR
                     ARQTIIPASSSSPGPGPTARASSGVPPAASTPVSAPSSAAPVPTPDAPARIVFEGGKG
                     SVHSRNGLVVAVEAAAARAGATVLESGGNAVDAAVATLFALCVTHPSAASLGGGGFAL
                     VRAPGGPTRAFDFRESAPAALNRTQFDAMQRVGARDARAVGVPGVVAGAAALHGAFAT
                     RSWADSIAPALRLARDGYVLGAHQARLIRASRPRFDRAAERAFPSELFKSGARVRQPD
                     LARTLERLATAGAPDFYAGEIAARLVSGLAPDGPTLADLRAYQALERTPLQIEYRGLT
                     LETMPPPSAGGIALIGTLLAQSEPALPRRSAAALHELLEAERRAQSQRKFAVADPARY
                     EAANARARELLWLSPAFWHQWPIDPEHATPSSRLTSSTDTSAESDHTTHLSVVDRNGM
                     VVSLTTTLSASFGAKVVAAGTGIVMNNSVASFSTSGENTPSPGRRTVSSMAPSLLLQG
                     GETRAVLGTPGGDTIPSTLAQVIRNLVDYRLPLDQAVEEPRFHQRFLPDEARYENRVP
                     LPRSVIKALEARGHRLRPGRAMGDAHCIVLDGLDAYGHADSRESGIAVPARQ"
     gene            complement(2876..>3168)
                     /locus_tag="VFQ61_25985"
     CDS             complement(2876..>3168)
                     /locus_tag="VFQ61_25985"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HET9957985.1"
                     /translation="GDVEINVENRGGTSASDIVVRIYAGDPSSGGELLGEALVAGPLE
                     PGDSITEHVDVGPLTRDIRLWAWADPADSIAECTDANNLAEGPLLNCNVIPR"
BASE COUNT          525 a         1003 c         1088 g          552 t
ORIGIN      
        1 gagttcgagg ctcaggcgct gaagagcgcc gggttcgagc tcgacgtggt gccgccgcgc
       61 taccgggccg cctacttctc tcattgtttc tcggacgcgc tggggtactc cgcgggttac
      121 tactcctata tctggagtga agtgctggcg cgcgatacgg aggcttggtt tggcgcccac
      181 ggcggcttgt cccggccggc gggcgacatt ctacgccagc agattctcgc caaaggtaat
      241 agtgtcgatc cgagccaggc gtttcaggcg ttctacggac gagctccaga catcgagcct
      301 ttgctggaaa aacgcggcct cttgccgtga aggtggcgcg tccgcgacga cgatttggcg
      361 ctagctctcg gtttggactg cggtgcccat agacagacac atcgagagca tcgacgaatt
      421 gagaccgcat gccgactaac gtcgcggcag cgtcgcttat cattggacgc gtgagcgtga
      481 ggtgatactc agctaggtcg tgtcggagtc gaagcgaccc actcttccgc cgggcgggca
      541 aaccgggaga atacggttcc agttcagggg gtcaaacgtg ctccaagcaa cttggtcggg
      601 ttacatcgat caagcctcct gtggccacta ccccgctgaa ctccggctaa tgttgggggg
      661 gcgtcggatt cggttcgcga tcttcgatgc gctcgcggtg actggatatg ccgcgaactc
      721 ccacgtgccg ggagggcaac tacttgccgt gtgtaaggac gcgggcgcgg agcgcgtgat
      781 tgtgatcacc gattcgagcg ccatacgcat gattggctcc acggtggcgc tcgcgactgg
      841 cctttccatt cggttcgtgg cgacagatgg ccaagccgtg agttggctgc gtgaacaggg
      901 ggtcgaagtc taggcgcctg cttcatttgc aggagatcta ggcgcttctt cgcgaagcct
      961 cattgtctcg cggggactgc gatccccgac tcacgagagt ccgcatgccc ataggcatct
     1021 agaccgtcga gcacgataca gtgcgcatct cccatggcgc gcccgggccg tagccggtgc
     1081 ccccgcgctt cgagtgcttt gatcacggag cggggcaggg gcacgcggtt ttcgtagcgt
     1141 gcttcgtccg gcaaaaaacg ttggtggaag cgcggctctt cgacggcttg atccaaaggc
     1201 agtcggtagt ccaccaagtt gcggatgact tgcgccagcg tgctcgggat cgtgtcgcca
     1261 ccgggggtgc ccaggacggc acgcgtctcg cctccctgga gtagcagact aggcgccata
     1321 gagctgacgg tgcgtcgacc tggggagggc gtgttctctc cgctcgtgct gaaggaggcc
     1381 accgagttgt tcatcacgat tccggtgccg gcggccacga ctttcgcccc gaacgaggca
     1441 gacaaggtcg tggtcaggct cacgaccatg ccgttgcgat ccaccactga gaggtgagtc
     1501 gtgtggtcac tctcggccga agtgtcggtc gacgatgtca acctactcga gggcgtggcg
     1561 tgctcgggat cgatcggcca ctggtgccaa aaggccgggc tcaaccagag cagttctcgg
     1621 gctcgcgcgt tggccgcttc ataacgcgct ggatccgcca ctgcgaactt acgctgcgac
     1681 tgggcgcggc gttccgcttc gagcagctcg tggagagccg ctgcgctccg gcgtggtaat
     1741 gccggctcgc tttgggcgag caaggtcccg atcagcgcga tcccaccggc agagggtggc
     1801 ggcatggtct cgagagtgag gccgcggtac tcgatctgga gcggggtgcg ctccagcgcc
     1861 tgataggctc gaaggtccgc gagcgtgggc ccgtccgggg ccaaacccga gaccaagcgc
     1921 gcggcgattt ctcccgcgta aaagtcgggc gcccccgcag tagcgagccg ctccaacgtt
     1981 cgagccaggt caggctgcct gaccctcgct ccgctcttga agagctcgct cggaaaagct
     2041 cgctctgccg cccgatcgaa gcgcgggcgg ctagcgcgga tcaagcgggc ttgatgcgcc
     2101 ccgagcacgt agccgtcgcg cgcgagtcgc aaggcgggag caatcgagtc ggcccacgag
     2161 cgagtggcga aggctccgtg taaggcagcc gcgcccgcga ccacgcccgg aacaccgacg
     2221 gcccgcgcgt cacgagcgcc caccctttgc atggcgtcga actgcgtgcg attgagcgcg
     2281 gccggcgcgc tctcacggaa gtcgaacgcc cgagtaggac caccgggggc gcgtaccaga
     2341 gcaaagcctc cgccgcccaa gctcgccgcg ctcgggtggg tcacgcataa cgcgaacagc
     2401 gtcgccaccg ccgcatcgac ggcgtttcca cccgattcca gcactgtggc gccggcgcgg
     2461 gcggccgccg cttctaccgc gacgaccagg ccgttgcgcg aatggacgga gcccttgcct
     2521 ccttcgaaca cgatgcgggc gggtgcatcg ggcgttggaa ccggagcggc ggaggaggga
     2581 gccgagaccg gagtcgacgc ggccggcggg actcccgagc tcgctcgagc ggtgggcccc
     2641 ggcccgggtg agctgctcga cgcggggatg atcgtctggc gagcgcgaag ccgctccgcg
     2701 agcatggcgc tcgacggagg actcgcgctc ccgaaggggg cgggagccgt gacaccctcg
     2761 gtgcgagctt gggaacaggc gctactgacg agcagccagc tccccagcca tgccacgagc
     2821 ctgagctctg ttcgatggcc ggggttcaca cgtgagaatg ctcgtttggg ctgctctagc
     2881 gcgggattac gttgcagttc agcagcgggc cctcggcgag gttgttggcg tcggtgcact
     2941 ccgctatgga gtctgccgga tctgcccacg cccacaagcg aatatcgcgg gtgagcgggc
     3001 cgacatccac gtgctcggta atggagtcgc ccggttcgag gggccccgcg accagcgcct
     3061 ctccgagaag ctcacccccc gagctcggat cgccggcgta gatccgcacg acgatgtcgg
     3121 aggccgaggt tccgccgcga ttctcgacgt tgatctccac gtctccgc
//
LOCUS       DASTKU010000741        14915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1708193, whole genome shotgun sequence.
ACCESSION   DASTKU010000741 DASTKU010000000
VERSION     DASTKU010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14915
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708193"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="VFQ61_25990"
     CDS             <1..1389
                     /locus_tag="VFQ61_25990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957986.1"
                     /translation="AEPSGFVAPSASNQATALAFVEARLPDGATHLSALFESLAKLTA
                     TSSDVAVVYIGDCEPTWGVVDANELRSLAARSLGRASFNPIVIGSAVDTALAQDLAQS
                     TQGRWLRAQQPEQLTHFVQRLRAPAVRSASVTVSAAPGATVFPAGQLPLEEGQELVLL
                     VKTPKGQDALKALAVQAAVQTASGPQTLDWLPRTPPAPTRFVAQRFGSQLIRSLERAN
                     APKEEVVTASLSHGVMSKYTSFLVLESEEAYARYAIERKAKQDAEAPRVTGGDLNSLD
                     ANGASISLDRIQPGDPEIYIDAPRDCVSVTVVFPFGETKRARFDPEANQGRGAWMVRF
                     LVDRETAEGVYEAQVYIVHRDGRQELRKVSYAVDHTAPTLQVQIRPVRRAPAGTFQVQ
                     VTQPGPKEQQDLRRVEIRTPDGRVQELVPVRWAEFRGTFRLAASPAGPAALRVVGIDQ
                     ALNHRTVDVELP"
     gene            1386..2849
                     /locus_tag="VFQ61_25995"
     CDS             1386..2849
                     /locus_tag="VFQ61_25995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957987.1"
                     /translation="MSRTRARELWRQVGIAACVVGVVRSVAAGEPSPAPTRTEVQSRR
                     WLATEDVRDCVALPERRWLAATAGGLVFGAGDVAERILTAREGLPDTQVERLTLTGNR
                     VRVDTRSGSVAVSLEDHTVVQTRPPSEAPALSAQLTTSSLEGLPPGVFPRKFHQSGNT
                     RCWATDSGLFLSWSGTPVHRWRVSGLPSGNISAVASGGDQLFIGTFDQGLFIVDREER
                     LVPGPESGLNPNINALAWDSSNSTLWAATARGVSLCRHGARGWACSRVGQSSNMHAVI
                     PLEHGGVVAGGDAGLEFFSAGGTRTGSFTRKQGAPFRAVWALAEGPKGTLFVGTTQGL
                     FWSATTDFSSARAQPSAWLRRASLATGDLTDDWVTALLVQTNMVHVGTYNAGLVSFER
                     KASHLQRVNVQAELGYVNPAGISALDGDTLAVATMDGLRVGSGKEFRTLPTLGRDVTA
                     VLPRPQPRTLESSVARAQGEYWVASRRGLELQRLDSR"
     gene            2981..3763
                     /locus_tag="VFQ61_26000"
     CDS             2981..3763
                     /locus_tag="VFQ61_26000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957988.1"
                     /translation="MKSRTWIAFVGGLVTALSITPAASAQARVWCAPELEALSEGMCF
                     YRPAQAAESEHPVLVLFLHSLVAVKSDWQWEQQRVLMSAAERHGFSLLMPRGRAGVGP
                     RSAGDVWAWPTSAAKQAEVESELVDEWLRARAQIEARNGRPFERLFVFGFSNGAYYAS
                     TLALRNRVAADGYGVFAGGSGGKYASILGSRTSERAPIFIGYGTKDPAWHDMVSLADT
                     LKKLGWRHRVKAEPVGHLVTETQLAAAMRFLGEHALAPARSK"
     gene            3820..4110
                     /locus_tag="VFQ61_26005"
     CDS             3820..4110
                     /locus_tag="VFQ61_26005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957989.1"
                     /translation="MTSPSLALRLWCGLLAGFAAGTLAWITVGLLTPGDFFANGNVAV
                     ALAPGAFAVGAAWMGYRRAKRGLAGWLLALAGVAALFWIFAPNGWWASPPPR"
     gene            complement(4259..5086)
                     /locus_tag="VFQ61_26010"
     CDS             complement(4259..5086)
                     /locus_tag="VFQ61_26010"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9957990.1"
                     /translation="MLESMDFRGRSVLVTGASAGLGREIAKLLAIRYGAHVLAVARRV
                     DRLEQLRDEVETAGGRMRVLGADLSNAADVERVIVECSKGDPLYGAVLSAAVTHFGNY
                     EDLEWSAFETMLRTNVTSTVRLATELLPRLERSNAGGGLLIVASMAGLSPVPYQAAYA
                     GTKAFLVNFSASLWHELRGRNVSITTYAPGGIATEMTAGQRFELLRRWLMPADQAALE
                     GIEAFRQRKYIHVPGAMNRIGGVLMKMLPGRLVGAQLARAYKRSLDAALSAEGATRR"
     gene            complement(5188..9078)
                     /locus_tag="VFQ61_26015"
     CDS             complement(5188..9078)
                     /locus_tag="VFQ61_26015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957991.1"
                     /translation="MWERQSLGHRIAAVALALAGVSTMVAAAAPAPSSSAVSLEPSAS
                     PRALGTTTAGSPAQSPPAGATSTTQVQSAEERLTRALHELLRDSTGAPVEQSAYSRPS
                     SVKEVVDAYLRSTKGLGGASRPRLLPLVVSLPDPTHASISMYYDQGLAALRAAVLALG
                     FRQGRFVLPARVERLRGGAKDASEPASVNHAEDHAPDSPGVMLFHDEDGNLIVALLVL
                     ESAVLGARLPELELALQAAWEIAQVGARSTPPPAVPVVAPFFSGTAQSLLQALSRVER
                     APERFTVFTGTATNPALEAWFARSLPNLRFQRTVPSHRELQHFALEVIERAEGPSLVA
                     CFSELGTGLTASSSLPNQEDEGRRCNAELPFPVHISELRSEQARANGGPPSLFAGLRR
                     TIQLKMDGPEARAGDALSALSPFTIVDDDVTLGQELQRICEQRYKYLIVHATDPSDVV
                     FLAQQSHKYCPDISIVTLGYDQLFVHPNASATMAGALVISSHAPTHEFVNHARSFPNS
                     VSQGFFHAVKSAAHTAWSLGAVDIPPGNSLDPTSKPRLFASQIHNARFWPIDSRALPP
                     RVAPQRLTPTVSAARPDPAERESPWPTSVRAQFIAWPILLLALCHFLVRAAQARARWR
                     RLEPHWLSALKKSVRVPLQHLRRVSPLLDLFLRDLSDDENHTPRLRSDLSALFRRASS
                     SWRLPQRSRLLIADLGLLLLVVLAVLPFLSASIVEFRSESRGAPPVWSWRMLPQRSDG
                     PGLLVYIGLIDALGTLGLIAFDLVALFVGLYNHFQRQLQARRAFEARSAAPSPNTIVM
                     SGDDPNPPSRHSDYDTHVRFSRWPTFVATALVTVTAGWLVYQVTHWCLQPLWASAPEA
                     VDREAYRVLRLLDPFGMSPAAPLLLLGTGIYVWSLYALCRIERLDRFPDEAPDASPSA
                     TYTALGCVWNELRFPWSHARYHLPLMLVVGILSWKFFTHTRSTFEHADYVAAFKLGFV
                     LLGALVAAELVRFLTLSGLLLEALRRLASEPMTDAYGRISANISGSFGLQLRARVPLS
                     YEFATCALACRTLERLQTQLQPTFGSHFDALKTACRDVESALLPPKPPPFDVQYALAR
                     TERNAHGKLFTAAARMHSVLIDLWRVRAASPELDILKLPRSELRPGGAHASEPTCVLL
                     RGAMKRDVLLWMRAAEEFVALRSATFIYQVLHELRHTLTFALLGATALVFSVTCYPFQ
                     PGRLLTVCSWAIVILAAACGFARMVSLEKSEVLSRIGGTKPAALELNRTFVSQLLLYV
                     ALPIGGAVVGLFPGLAEVMAVQLSSLVQLLPAGK"
     gene            9268..9447
                     /locus_tag="VFQ61_26020"
     CDS             9268..9447
                     /locus_tag="VFQ61_26020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957992.1"
                     /translation="MSARIATLLDEVRAKILLGCESIVRLAAQREVLHRVLAASRKRF
                     HVVELEPMCLSATLP"
     gene            complement(9444..10178)
                     /locus_tag="VFQ61_26025"
     CDS             complement(9444..10178)
                     /locus_tag="VFQ61_26025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957993.1"
                     /translation="MGAHDITRDWGSGSPFVLPVELSDGELLNATRSLVGRSNQLLAL
                     LLGHLAELEARGLHRTRACSSLYTYCIYELRLSEDEAYRRVAAARLVRRFPALLDAVA
                     SGELHLTGLLMLGPHLSLDNLTEVLARAKHRTKKEVARLVRQLDPLPDVPARCGLEQK
                     HTRVQNKPPQKSRPRPRFRCGTWPCEVPPNRTTFGSVLATFRSSRASAGDMRQSSRAS
                     AGDMCRRPFGAPSSSATRGAAHSLMA"
     gene            10573..10968
                     /locus_tag="VFQ61_26030"
     CDS             10573..10968
                     /locus_tag="VFQ61_26030"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HET9957994.1"
                     /translation="MVTVRGLRGERGGLGELPSALVTARAVESRSSPDPGPETQLLNR
                     GERLMDTRNPKDSPVKIKGIKKIGEALYDIEFTIDGELPGDEGETIDWIEALKPQLPV
                     VAPPKVGRNQPCPCGSGKKFKKCCDGKAA"
     gene            11515..>14915
                     /locus_tag="VFQ61_26035"
     CDS             11515..>14915
                     /locus_tag="VFQ61_26035"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HET9957995.1"
                     /translation="MTVTPIQPVRYHHPPSPNPPAPVFATTQSATANAVLSTLDQAVA
                     PLRELPNSEDGAAGKVSKAVGGALGVINAPAMFIDTAASIGASKLDKFLASKLGIPTL
                     FPALPVARIGISMHMGTPHTHVHPPSTTPPAPPIPLPSFGVAFCSGSASVLVNGVPAL
                     RAGDVGIGLTCGSMAPPFEIMTGAAGVYFAGARVARLGMDITFHCNPSPATAAAAAKV
                     AKPFMNVGKAAVAMGAAGAIAGGAGAIDAMEKGNSAAAAVQAAQMALDAAALALSMLR
                     GKDPAGPPGVGVLIGSPPTVLAGGAPIPNIGALAQGRLFSQIGKAVRGLKNKIKRKSP
                     SKDANGGCGRAGEPVNLITGENFNTYIDFKSYHGAFTWQRYMTSAWARQPGRLGPCFK
                     HVYETRLEIRLHRVVFFGFSGEHIEFPRLAPHKTEVSVNGYRLTRISETRFRVTERRL
                     GTLEFQRPHERTATAYPVASQNDEARAELIYRADGLLTEIREYSIGLLTPLAVYALGY
                     DTAGRLLTIHGANLAGQRVPTERVDWQRLAVYSYDSAGHLLSAEDAFGARESYQFDGR
                     HRLARATDRAGYSFEWTYDLEDRCITSAGQDGLWFVKIEYAPAARQTQVTFHHGGTMV
                     VQYDEDGVVTKVIDANGAELERQRAEDGRVVAEVDSAGRRMAFLYDVDGANIGRVDRF
                     GHRYLPELDQPWIGNPFEPQLPENPQERQFGMHAAPSAEADVGRVLDTLPKELTGTAH
                     QLFELNNPARFDATLRTERDIYGRTLRETDVLGRAREWRYDSVGNEIMRLDRDGRLHQ
                     QRFTSWGLRTETLDPLGNTVRYAYDPHENITRIVDPLGTTTEYGYDKKDRLIQVSRHG
                     RVRETYVRDRDGRLIEKRGTDGQPLLKFTPHPNSLAAKIELASGGQVALDYDARGQVT
                     TASTDQHQVTQAWDSLRRLLHANFDGHEVTHSYALGQRTLTRIGGRFTISYDRSLPVT
                     DFFADPFTVGAEAQSAELRIVGPSGFTWTILRGERGILRVRHGNAPTAAQEIQQYTRE
                     GKLQARLVWRSVRNPRNATLDTAHWTTRYEYSPEGDLLEVRDSQRGKRQFEIDAAHRL
                     TAELTPDGRRLNYPLDEAGNLLSKPDLPLVRIERGNRLQHAGLES"
BASE COUNT         2731 a         4701 c         4907 g         2576 t
ORIGIN      
        1 gccgagccga gtggcttcgt cgctccgtca gcatccaatc aagcgaccgc actcgccttc
       61 gtggaggcgc gcttgcccga tggagcgacg catttgagtg cgttgttcga gtccctggcg
      121 aagctgacgg ccacctcgtc cgatgtggcg gtcgtttaca ttggcgactg cgagccaacg
      181 tggggagtgg tggatgcaaa cgagctccga tctctggcag cgcggagcct ggggcgagcc
      241 agcttcaacc cgatcgtgat cggctcggcc gtcgacacgg ctttggccca agacttggcc
      301 caaagcaccc aaggccgctg gctgcgagct caacagcccg agcagctcac gcacttcgtt
      361 cagcgcttgc gtgctccggc cgtgcgctcc gcgagcgtga ccgtcagcgc ggcgcccggc
      421 gcaacggtgt tcccagctgg ccagctgccg ctcgaggaag ggcaagagtt ggtcttgctc
      481 gtcaagacgc cgaaagggca agacgcgctg aaggctctgg ccgtacaggc ggcggtgcag
      541 acggcctcgg gcccgcaaac gctggactgg ctgccgcgca cgccgcccgc gccgactcgc
      601 ttcgtggccc aacgcttcgg gtcgcagctg attcgatcgc tggagcgtgc gaacgctccc
      661 aaagaagaag tcgtcaccgc atccttgagc catggagtca tgagcaagta cacttctttc
      721 ctggtgctcg agagcgagga ggcctatgcg cgctacgcga tcgagcgcaa ggccaagcag
      781 gacgccgagg ctccgcgcgt tacgggtggc gacctgaatt cgctcgacgc gaacggcgcc
      841 tcgatcagcc tggatcgcat tcaaccgggt gaccccgaga tctacatcga cgcgccccgg
      901 gattgcgtga gcgtgaccgt ggtgttcccg ttcggagaga ccaagcgcgc ccgcttcgac
      961 cccgaggcga atcaaggccg gggcgcgtgg atggtgcgct tcctcgtcga tcgtgagacc
     1021 gccgagggcg tttacgaagc acaagtctat attgtgcatc gcgacggtcg ccaggagctg
     1081 cgtaaggtct cttatgccgt cgaccacact gcgccgacgc tgcaggtgca gattcgtccg
     1141 gtccggcgcg cgcccgccgg cacgttccag gttcaagtga cgcagcctgg gcccaaagag
     1201 caacaggatc ttcgccgtgt ggagatccgc actccggatg gccgtgttca ggagttggtg
     1261 ccggtgcgct gggcggagtt tcgcggcaca tttcggctgg ctgcgtcgcc tgccggaccg
     1321 gcggcgttgc gcgtggttgg gatcgatcaa gcgctgaatc accgcacggt ggacgtggag
     1381 ctcccgtgag tcgcacgcga gcacgggagt tgtggcggca ggtcgggatt gcggcatgcg
     1441 tcgtgggtgt cgttcggagt gtcgcagcgg gagagcccag cccggcgcca actcgaactg
     1501 aggttcagtc acgacgttgg ctcgcgaccg aggacgtccg cgactgcgtc gcgctgcccg
     1561 agcgacgctg gttagcggcg acggcgggag ggctggtgtt cggtgcgggg gacgtcgcgg
     1621 agcgcatcct caccgcccga gaaggtctgc cggacaccca agtggaacgg ctcacgctca
     1681 ccggcaatcg cgtgcgcgtc gacacccgat cgggctcggt agcggtgtct ctcgaagacc
     1741 ataccgtggt gcagacgcgg ccgccgagcg aagcgccggc gctgagcgct caactcacga
     1801 cgtcgtcgct cgaggggttg ccgccgggcg tattcccacg gaagtttcat cagtccggaa
     1861 acacgcggtg ctgggccaca gactccggac tattcctctc gtggagcgga actcccgtgc
     1921 accgctggcg tgtgtcggga ctgccgagtg gcaacatcag cgcggtggcg agcggcggag
     1981 accagctctt cattggcact ttcgatcaag ggctattcat cgtcgatcgt gaagaacggc
     2041 tcgttcccgg ccccgaatcg ggcctcaacc cgaacatcaa tgctctggcc tgggattcct
     2101 cgaatagcac tctctgggcc gctactgcgc gcggagtctc actctgccgc cacggtgcgc
     2161 gtggctgggc ctgcagtcgt gttggtcaat cgagcaacat gcacgccgtg atcccgctcg
     2221 agcacggcgg cgtcgttgct ggtggcgacg cgggcctcga gttctttagt gccgggggca
     2281 cgcgcacggg cagcttcacc cgcaagcaag gtgcaccatt ccgagcggtc tgggcgctcg
     2341 cggaggggcc caagggcacc ttgttcgtgg gcaccaccca gggcttgttc tggtccgcca
     2401 ctacggactt ctccagcgcg cgcgcgcagc ccagcgcctg gttgcgccgt gcctcgcttg
     2461 ccactggcga tctgacggat gattgggtga cagctctctt ggtgcagacg aatatggtcc
     2521 acgtgggcac ctacaacgcg ggcctcgtca gcttcgagcg caaagccagc caccttcagc
     2581 gcgtcaacgt tcaggcagag ctcggatacg tcaatcccgc tgggatcagc gccttggatg
     2641 gagacacgtt ggcggttgcc accatggatg gcctgcgtgt aggtagcggc aaagagttcc
     2701 gaactctgcc gacgctcggc agagacgtta ctgcggtctt gccgcgtcct cagcctcgaa
     2761 cgctcgaatc atcagtcgcg cgagcacaag gcgaatattg ggttgcctct cggcgtggtc
     2821 tcgagctcca gcgactcgat tcccggtagt tgtgtcgtat cgagatcttc tcaccgccaa
     2881 tcgccgcaga atcggaactt tgcgccactg ctgagtggaa aagcaacggc tccgcgaatt
     2941 tgtactcgac cttcgttgct cttggggcat gttggcggcc gtgaagagcc gcacttggat
     3001 cgcattcgtc ggaggccttg tcaccgctct ttcgattacg cccgcggcca gcgctcaagc
     3061 gcgcgtgtgg tgcgcgcccg aactcgaagc cttgtcggaa ggcatgtgct tttaccgccc
     3121 agcgcaagcc gccgagtccg agcaccccgt gctcgtgctg ttcctgcaca gcctggttgc
     3181 cgtgaagtcg gattggcaat gggaacagca gcgtgtccta atgagcgctg cagagcgcca
     3241 cggcttctcc ctgctcatgc caagggggcg agccggcgta ggcccgcgga gcgccggaga
     3301 tgtatgggcc tggccgacgt cagcggccaa acaagccgag gtcgaatcag agctcgttga
     3361 cgaatggcta cgcgcgcgtg cccaaatcga ggcccgcaac ggtcggcctt tcgagcggct
     3421 attcgtcttc ggcttttcga atggcgccta ctatgcgtcc accttggcgc tccgcaatcg
     3481 cgtggctgcc gatggctacg gcgtcttcgc cggaggcagc ggaggcaagt acgcttccat
     3541 cttgggcagc cgcaccagcg agcgcgcgcc catcttcatt ggttacggca ccaaagatcc
     3601 ggcttggcat gacatggtgt ctctcgcgga cacgctcaaa aagctcggct ggcgtcatcg
     3661 agtcaaagcc gagcccgtcg ggcacctcgt cacagaaacc caacttgctg ccgccatgcg
     3721 atttctcggc gaacacgccc tagcgccagc gcgaagtaag tgagcaatgt ctctgttttg
     3781 ggccagcacg gagtttaggg tcgccttgtc tcaaatgcca tgacttcgcc gagccttgct
     3841 cttcgacttt ggtgtggact actcgcgggt tttgcggccg gcacactggc ctggatcacg
     3901 gtgggcctgc tgacgcccgg agacttcttt gcgaacggta acgtggccgt cgcgttggcg
     3961 cccggcgcat tcgcggtggg cgccgcttgg atgggctatc ggcgcgcgaa gcgaggcttg
     4021 gcagggtggc tgctcgcgtt ggcgggcgtt gccgctcttt tttggatatt cgcacccaac
     4081 ggctggtggg caagcccacc tccacgctaa acgatccgtt tcagcgaagt ggacgcggag
     4141 ttgagtcccg cgttctgccc atcggacttc gatgatagtg gtttggtccg tccactcgta
     4201 gaacctcttg gcgttctctt tctgacgcgg gcgtcccgtc gtgcacccaa ctctgcaatc
     4261 aacggcgcgt tgcgccttcg gcactcagtg ccgcatcgag ggaccgcttg taggctcggg
     4321 cgagctgagc gccgaccagt ctgcccggaa gcattttcat cagcactcct ccaattcgat
     4381 tcatcgcgcc tggaacgtga atgtacttac gctggcgaaa cgcctcgatc ccttccagcg
     4441 ccgcctggtc ggcgggcatg agccatcggc gtaagagctc gaagcgctgc ccggctgtca
     4501 tctccgtagc aatgcctcct ggagcgtatg tggtgataga cacattgcgg ccgcgaagct
     4561 cgtgccacaa actggcgcta aagttgacga ggaaagcctt tgttccggcg taggcggcct
     4621 gatacggaac gggagacaac ccggccatac tcgcaacaat cagcagccct ccacccgcat
     4681 tgctacgttc aagcctgggc aatagctcgg ttgcgagtcg cacggtgctg gtcacgttcg
     4741 ttctcagcat ggtctcgaat gctgaccact cgaggtcttc gtaattgcca aagtgggtga
     4801 cggcagcgct cagaacggcg ccatatagcg ggtcaccctt cgaacactcg acaatgaccc
     4861 gctccacgtc cgcggcgttg gacagatcgg caccgagaac tctcattctg ccgccagcgg
     4921 tctctacttc atctcgaagt tgctccaagc ggtccacacg acgcgctacc gcgaggacgt
     4981 gcgcgccata tcgtatcgcg agcaacttgg ctatttcccg tccgaggcct gcggacgcgc
     5041 ccgtcacgag aacggagcgg ccgcggaagt ccatagattc gagcacgagt ctattcaaca
     5101 cacttgggcc gagggcgcca tgggccggcg cttcggacgg cttcaaaacg atgccctggt
     5161 aatacaacgg gggcggcgat cacggcatta cttgcctgct ggaagcagct gaaccaggga
     5221 agagagctgc acggccatga cctcggcgag gccggggaag agccctacca cggcgccgcc
     5281 aatgggcaac gcgacgtaga gcaggagctg agagacgaaa gtgcgattga gctcgagcgc
     5341 ggccggcttg gtcccaccaa tgcgactcag tacctcgctt ttttccaatg acaccatgcg
     5401 tgcgaagccg caagctgcgg cgaggatcac gatcgcccag ctgcaaacgg tgagtaggcg
     5461 acccggttgg aacggatagc aagtcacact gaatacgagg gccgtggcgc cgagcagcgc
     5521 gaaggtgagg gtgtggcgga gctcgtgcaa cacttgataa atgaacgtag cggagcgaag
     5581 cgcgacaaat tcctccgcgg cgcgcatcca gagcaggacg tcgcgcttca tggcccctcg
     5641 cagcaacacg cacgtaggct cgctcgcgtg cgcgccgccc gggcgcagct ccgagcgggg
     5701 gagtttcagg atatcgagtt ccggcgacgc ggctcgaacg cgccaaaggt ctattaggac
     5761 gctgtgcatc cgcgcggcgg ccgtgaatag cttgccgtgt gcgttccgct cggtgcgcgc
     5821 cagcgcgtat tggacgtcga acggcggcgg tttcgggggg agcagggcgc tctccacatc
     5881 ccggcaggcg gtctttagcg catcgaaatg ggagccgaac gtaggttgga gttgagtctg
     5941 tagccgctcc aaggtgcggc aggcgagcgc acaggtcgcg aattcataag acaacgggac
     6001 tcgcgcgcgg agctgcaagc cgaagctgcc cgagatattg gcggagatgc gcccataggc
     6061 gtccgtcatc ggctcggagg cgagccggcg cagcgcctcc agcagcaggc ccgagagggt
     6121 tagaaatcgt acgagctcgg cggcgaccag cgcgcccaat aacacgaagc ccagcttgaa
     6181 ggcagcgacg tagtcggcgt gctcgaaggt cgaccgcgtg tgagtgaaga acttccagct
     6241 caggatgccg acgacgagca tcagtgggag gtgatagcga gcgtgggacc aggggaagcg
     6301 cagctcgttc cagacgcagc caagggccgt ataggtggcg ctcgggctag cgtcgggcgc
     6361 ctcgtctggg aagcgatcca gtcgctcgat gcggcacaag gcatagagcg accatacgta
     6421 gattccggtc ccgagcagga gcagcggggc tgcgggcgac ataccgaacg ggtccagcaa
     6481 ccgcagcacc ctgtacgcct ccctatcaac cgcttcgggc gcgctggccc acagcggctg
     6541 caagcaccaa tgagtcacct ggtagacgag ccaacccgcg gtcaccgtca cgagagcggt
     6601 cgctacgaac gtgggccagc gcgagaagcg cacgtgggtg tcgtagtcgc tgtgccgtga
     6661 gggcgggttc gggtcgtcgc cagacattac gatcgtgttc gggctcggcg cggcgcttcg
     6721 agcttcgaat gctcttcgtg cctggagttg acgttggaag tggttgtaca agccgacgaa
     6781 gagcgccacg agatcgaagg caatcaagcc aagcgtgccg agagcgtcga tcaatccgat
     6841 atagaccaag aggcccgggc cgtcgctgcg ctgggggagc atgcgccagc tccagaccgg
     6901 tggtgcgccg cgcgattcgc tacggaactc cacgatactg gcgctcaaga acggcaatac
     6961 ggcgagcacg acgagcagga gcaggcccaa gtccgcgatc aggaggcgcg aacgctgggg
     7021 gaggcgccac gaagagctgg ctctacggaa caacgccgat aaatcgctgc gcagccgagg
     7081 cgtgtggttc tcgtcatcgg agaggtcacg caggaataga tcgagaaggg ggcttacgcg
     7141 gcgcaggtgt tggagcggga cacgcacgct cttcttgagc gcactcaacc agtgcggctc
     7201 cagccggcgc caccgtgcgc gtgcctgggc ggcgcgcacc aagaagtggc agagcgccag
     7261 cagaaggatg ggccaggcga tgaactgcgc tcgaacggag gtgggccagg gggactcgcg
     7321 ctcggcggga tctggccgcg cggcgctgac cgttggagtt agcctttggg gagctaccct
     7381 ggggggtaga gcgcggctgt cgatgggcca gaagcgggcg ttgtggatct gagaggcgaa
     7441 cagacgaggc ttggatgtgg ggtcgaggct gttgcccggc gggatgtcga cggcgccgag
     7501 gctccaggcc gtgtgcgcgg cgctcttcac ggcgtgaaag aagccttggc tcaccgaatt
     7561 cggaaaggag cgagcgtggt tcacgaactc gtgggtcggc gcgtgactcg agatcacgag
     7621 cgcgcccgcc atggtcgcgc tcgcgttggg gtgcacgaag agctgatcgt acccgagcgt
     7681 cacgatcgag atatctggac agtacttgtg cgactgctgc gctagaaaca cgacgtcgct
     7741 cggatcagtg gcgtggacaa tcaggtactt gtagcgctgc tcgcaaatcc gctgcagctc
     7801 ttgaccgaga gtaacgtcgt cgtcgacaat cgtgaacggt gacagcgcgc tgagcgcatc
     7861 acccgcgcgg gcctcgggac cgtccatctt gagctggatg gtgcgccgca gcccagcgaa
     7921 caaggatggc gggccgccgt tcgcgcgggc ctgctcggag cgcagctcgg agatgtgaac
     7981 gggaaacggg agctctgcgt tacagcgccg cccttcgtct tcctggttcg ggagcgagct
     8041 cgaggcggtg agcccggtgc cgagctccga gaagcacgcg acgagcgacg ggccttcggc
     8101 tctctcgatc acctcgagtg cgaagtgttg cagctcacgg tggctcggga ccgtgcgttg
     8161 gaagcgcaga ttcggcaacg agcgagcgaa ccacgcctct aatgctgggt tggtggcggt
     8221 gccggtgaat acggtgaacc gctcgggtgc acgctcgact cgactcaacg cttggagcag
     8281 cgactgggcg gttcccgaaa agaagggggc aacgacgggg accgccggtg gcggcgtgct
     8341 tcgggcaccg acctgcgcga tctcccaggc tgcttggagc gccaactcga gctctggcaa
     8401 gcgcgcgccc aacaccgcag actcgagcac tagaagtgca acgattagat tgccgtcctc
     8461 atcgtggaac agcatcacgc cgggcgaatc tggggcgtga tcctccgcgt ggtttacgct
     8521 tgcgggttcc gacgcatctt tcgcgccccc tctgagccgc tctacccgag ccgggagtac
     8581 gaagcgcccc tgtcggaagc cgagcgccag caccgccgcg cgcagagctg cgagcccttg
     8641 gtcgtaatac atcgagatag aggcatgggt aggatcgggg aggctgacca cgagcggcaa
     8701 caagcggggc cgactcgcgc cgcccagccc tttcgtcgag cggagatacg cgtccaccac
     8761 ttctttcacc gagcttggcc gtgagtacgc gctctgttcg acaggcgcac ctgtcgaatc
     8821 ccgcaacaac tcgtgcagag cgcgagtcag ccgctcctca gcactctgca cctgagtggt
     8881 cgaagtcgcg cctgcgggag gagactgcgc ggggctgcct gcggttgtcg tccctaatgc
     8941 gcgcggtgac gcactgggct ccaggcttac ggcagagctc gacggcgcgg gggccgccgc
     9001 ggccaccatc gtcgacactc ccgccagcgc gagtgcgact gccgcgatcc ggtgacccaa
     9061 cgactggcgt tcccacatct gtcctccgca cgggcgactc tcgaagacac ccggccagcc
     9121 tcatcgaacc gaatccgtct gaacggagca atggtcattt ccccagaagt gaacccgcga
     9181 agtggaagtg cgcgggcgac gatatcctgg tcgccactgc ccagaccgtt cggtgctcca
     9241 gcgtcatgtc tgacgtttgc agccctcgtg tcggctcgaa tcgcgacgct tctggatgaa
     9301 gtccgcgcca aaatcctgct cggctgcgag agcattgtgc gcctggcagc gcagcgcgag
     9361 gttctccacc gtgtgctcgc cgcctcgcgc aaacgcttcc acgtggtgga gctcgagccg
     9421 atgtgtctct cggcaacgct gccctaagcc atcaatgaat gtgcagcgcc cctggtcgcg
     9481 ctcgaagacg gcgcgccgaa tggccgccgg cacatgtcgc ccgcgctggc gcgagagctc
     9541 tgcctcatgt cgcccgcgct ggcgcgagag ctccggaacg tcgccagtac gctcccgaac
     9601 gtcgttcggt tcggcgggac ctcgcagggc cacgttccgc accgaaaacg agggcgcggt
     9661 ctgcttttct ggggcggttt gttctgcacg cgcgtgtgct tctgctcgag gccgcagcga
     9721 gccggaacgt ctggcaacgg gtcgagttgc cgaacgaggc gcgcgacttc tttcttcgtc
     9781 cgatgcttcg cccgggccag cacctcggtc agattgtcga ggctcaggtg tggaccgagc
     9841 atcaacaagc cggtgaggtg gagctcgccg gaggcgaccg cgtcgagcaa agccgggaag
     9901 cggcgaacca gtcgcgccgc cgctacgcgt cggtaggctt cgtcctccga caatcgcagc
     9961 tcatagatgc aatacgtata gaggctcgag caggcgcgag tacggtggag cccgcgcgcc
    10021 tccagctcag cgaggtgacc gagcagtagc gccagcagct gattggaccg acccacgagg
    10081 cttcgtgttg cattgagcaa ctcaccgtca gacagctcga ccggcaaaac gaacggagac
    10141 cctgaacccc aatcccgggt gatatcgtgt gcgcccatga actccatctt acaccctagt
    10201 tttcggagct cgatattcgc gctctcgtgc gtcctcgcgc gcagacacga cgttcgagcg
    10261 gtatgaccgt ccgaaagcag cacaacgggt catacgcgcg cggcaaacgc gaaagatccg
    10321 cactcgtgca tcgaacggca gacggcactc gccaaatcgt gtcaagcgtt gaattcgaaa
    10381 tctgcgcgtc gtgacccagc acttcagccg cgcgcttcgt actactcatc acggtcgtgg
    10441 acgtcatcgc ggtctggggt gtggtcctgc cgctcggtga gtaaattact gatggttcga
    10501 cttttcacgc aaccggttgc gttcagctgc cgataggcgg ggtgaatggt caaggtctga
    10561 gctcgagagc gggtggttac agtgcggggc ctgcgcggtg agcgcggggg cctgggtgaa
    10621 ctcccgtctg cgctggtgac ggcacgcgcc gtcgagtcgc gctcgtcccc agatccgggc
    10681 ccagaaacac agttgctcaa ccgaggagaa cgtttgatgg acacacgaaa tcccaaagac
    10741 tcacccgtga agatcaaagg catcaagaag attggagagg ccttgtacga tattgaattc
    10801 acaatcgatg gcgaattgcc cggcgacgag ggagagacga tcgattggat cgaggctctg
    10861 aaaccgcagc tgcctgtagt ggctccgccg aaagtggggc gaaatcagcc atgcccctgc
    10921 ggcagtggga agaagttcaa gaagtgctgc gatggcaaag ccgcgtgatg gagctggtcg
    10981 agacgcggtg accctttgga atgcgcggcg tagaaggacg acgtcaccgt gagtgacggc
    11041 tgatggattc cgagcgcgcg gccaaccttc gcacgagggc gtcgagtcac cgcgaccctt
    11101 aaggacgaag acgggctcca gaggcgcacg gcggctcgcc ctccaggttt cgtcgagcga
    11161 gctgccatga gctcaagcgc gggaccgtcc ggcgacccgc ctcaggcccg gcgatctttt
    11221 cgaagcggag ggaagagttt gaagtaaact cccgtactgg gtttgggtat gggttgcctg
    11281 ggtcagggtc gtggagttgg attcgatgaa cattgggtcg gatggttggg ggacggtgcg
    11341 ttgggcggcg tgcctcggga cggcgggctg gtatgagaag ggcccctgcc gcatgaattc
    11401 ccgccgccgc gccgcgctca cggatcgagc ccctgaggtc agcggtcgtc ggttcgcggg
    11461 caggggcctg ttcgcagatt cgtccattgg gagttcggtc gcgcgaggtg cggcatgacg
    11521 gtgactccga tccagccggt tcggtaccat cacccgccgt ccccgaatcc gcccgcgccg
    11581 gtgtttgcga caacgcaaag cgcgacggcg aatgcagtgc tgagcacgct ggaccaagcg
    11641 gtagcgccct tgcgtgaact gccgaacagt gaagacggcg cggccggtaa ggtttcgaag
    11701 gcggtgggcg gggcgctcgg cgtgatcaac gcgccggcga tgttcatcga tacggcggcg
    11761 tcgatcggcg cctccaagct agacaagttc ctggcgagca agctggggat ccccacgctg
    11821 tttccggcgc tgccggtcgc gcgcatcggg atcagcatgc acatgggcac gccgcacacc
    11881 cacgtgcacc cgccgagcac cactccgccc gcgccgccga ttccattgcc gagctttggg
    11941 gtcgcgtttt gttcgggctc ggcgtcagtg ctggtgaacg gggtgcccgc gcttcgcgcg
    12001 ggcgacgtgg gaattggcct gacgtgcggc tcgatggcgc cgccattcga gatcatgacc
    12061 ggcgcggcgg gcgtctactt cgcgggtgcc cgcgtggcgc gccttggaat ggacatcacc
    12121 ttccactgca atcccagccc tgccaccgcg gccgcggcgg ccaaggtggc aaagcccttc
    12181 atgaacgtgg gcaaagccgc ggtcgcgatg ggagctgcgg gagcaatcgc cggcggggcg
    12241 ggcgccattg acgccatgga gaagggcaac agcgccgcgg ccgccgtcca agccgcgcaa
    12301 atggcgctcg acgcggcggc cctcgccctg tccatgctgc gcggcaagga tcccgcaggc
    12361 ccccccgggg tgggcgtgct catcggctca ccgcccaccg tcctcgcagg cggcgcgccc
    12421 atccccaata tcggcgcctt ggcgcagggc agactattca gccaaatcgg caaagccgtc
    12481 cgagggctca agaacaagat caagcgcaag tcaccgtcga aggatgccaa tggcggctgc
    12541 ggccgcgccg gtgagcccgt gaatctgatc acgggcgaga acttcaatac gtatatcgac
    12601 ttcaagtcgt atcacggcgc tttcacgtgg cagcgctaca tgacgagcgc ttgggcccgt
    12661 caacctgggc ggctggggcc ttgcttcaag cacgtgtacg agacgcgact cgagattcgc
    12721 ctgcatcgag ttgtgttctt tggcttctcg ggggaacata tcgagtttcc tcggctcgcg
    12781 ccccataaaa ccgaagtttc ggtcaacggc tatcggctca cacgaatctc ggagacgcgc
    12841 tttcgcgtca cggagcggcg acttggtacg ctcgagtttc agcgcccgca tgagcgcacc
    12901 gcgaccgcct accccgtcgc ctctcaaaac gatgaggcgc gcgccgaact catttatcgt
    12961 gccgacgggc tgctgaccga gattcgcgag tactcgattg gattgctcac accactcgcg
    13021 gtgtatgcct tgggctacga tacggcgggt cgcctgctga ccattcacgg tgccaatctc
    13081 gctggccagc gcgtgccgac cgaacgagtg gattggcagc ggcttgcggt ctattcgtac
    13141 gacagcgcgg gccatctgct aagcgccgag gatgcgttcg gggcgcggga gagctaccaa
    13201 ttcgatggtc gacatcgcct ggcccgcgcg accgatcgcg cgggctactc cttcgagtgg
    13261 acttacgatc tcgaagatcg ctgcattacc agcgcaggcc aagatggcct ttggttcgtg
    13321 aagatcgagt acgctcccgc ggcgcgacaa acgcaggtca cgttccacca cggtgggacg
    13381 atggtcgtgc aatacgacga agacggcgtg gtcaccaagg tgattgacgc gaacggcgcg
    13441 gagctcgagc gccagcgcgc cgaagacggc cgcgtcgtcg ccgaggtgga ctcggccggg
    13501 cgccgcatgg cgttcttgta cgatgtcgac ggtgcgaaca ttgggcgcgt cgaccgtttt
    13561 ggtcatcggt atttgcccga actcgaccag ccctggatcg gcaatccctt cgagccgcag
    13621 ttgcccgaga acccgcaaga gcgccagttc ggaatgcacg ccgccccgag cgctgaagcg
    13681 gacgtagggc gggtgctcga cacactcccg aaagaactga ctggcacggc gcaccagctc
    13741 ttcgaactga acaaccccgc ccgcttcgac gccacgctgc gcaccgagcg cgacatttac
    13801 ggccgcaccc tgcgcgaaac cgacgtattg gggcgtgctc gcgagtggcg ctatgactcc
    13861 gtcggcaacg agatcatgcg cctcgatcgc gacgggcgcc tccatcaaca gcgcttcacg
    13921 tcatggggct tgcgcaccga gaccctggat ccgctcggca acaccgttcg gtacgcgtat
    13981 gaccctcacg agaacatcac gcgcatcgtc gacccgctcg gcaccaccac cgaatacggc
    14041 tacgacaaga aagatcgtct catccaggtc agtcgacatg gtcgagttcg ggagacctac
    14101 gtacgggacc gcgatgggcg cctgatcgaa aagcgcggca ccgatggcca acccctcctg
    14161 aagttcacgc cccacccgaa ctccctcgcg gcgaaaatcg aactcgcgag cggcggccaa
    14221 gtcgcgctcg actacgacgc gcgggggcaa gtcacgaccg ccagcaccga ccagcaccaa
    14281 gtcactcaag cgtgggactc actgcgtcgg ctgctccacg ccaacttcga tggacacgag
    14341 gtcacgcact cctatgccct cggacagcgc accctgacgc gcatcggcgg gcgttttacc
    14401 atcagctacg atcgaagcct ccctgtcact gacttcttcg ccgacccatt cacggtcggg
    14461 gcggaagccc agtctgccga actccgcatc gtcggcccga gtggcttcac ctggaccatc
    14521 cttcgtggcg agcgcggcat cctgcgcgtt cgccacggca acgcccccac cgccgcgcag
    14581 gagattcaac agtacactcg agagggcaag ctccaagcgc gcctcgtctg gcgatccgtg
    14641 cgcaaccctc ggaacgccac cctcgacacc gcgcactgga ccacgcgtta cgaatacagc
    14701 cctgaaggcg acctcctcga agtccgcgac agccagcgtg gcaaacgcca gttcgaaatc
    14761 gacgccgcgc accgcctcac tgccgagctc acgccggacg gtcgccgtct taattatcca
    14821 ctcgacgaag cgggaaacct cctcagcaaa cccgatctac ccctcgtccg aatcgagaga
    14881 ggcaatcgcc tccagcacgc cggactcgaa agctt
//
LOCUS       DASTKU010000742        16213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1708196, whole genome shotgun sequence.
ACCESSION   DASTKU010000742 DASTKU010000000
VERSION     DASTKU010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16213
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708196"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFQ61_26040"
     CDS             complement(<1..379)
                     /locus_tag="VFQ61_26040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957996.1"
                     /translation="MRSLLVWSSLLGATALHGCSSDEVDSTTATGGSAGNRVSTGGAG
                     AAAPKSTTSGVASGGAGHSTAGGVSAGGSSSVMTSGGVAHSSAGDANAHSGGTGGGSS
                     HNPLMGGRTQGGALNESGGRVDAG"
     gene            complement(531..2876)
                     /locus_tag="VFQ61_26045"
     CDS             complement(531..2876)
                     /locus_tag="VFQ61_26045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9957997.1"
                     /translation="MPQLGRNYWIMAAILAYASLAAARTTSGPLPWLALVALPLLLSE
                     VFRRTEQVPGDDRYALEARSGVRLTGFGAALLIAARTGESAQPALDALANLGAGASAV
                     GSLLALSRLPALAGLLDVHRAARSLDAVLFTGFLWGLATAVPAGYAILPPSALRFDPL
                     VIDYVTSSAGIGSLLVMVAATLRLRFLRRLELGTGDRVRSAFALCIAGFTVAVPAAWL
                     DVAAPDRLLPATVVVVSSACAWASITREATLVTRMLRGVLALTLLGAPLLVAATTLSK
                     LMPEQAPAVALVSGIFAVSVGLAGHAVARPLGPEQSRWLVALDRAARDALQPEPQAAL
                     RAALLALGKLSGSDTQGAEIWQKDPPDVLSVDVAGYLTVTRSEAPERIYELGMQEPER
                     TLRADILKALEVKRAEVRGVLAWLESRKAFSATIVVDDDGPVGFVLLPRAGRSSPLTL
                     EEARAARLLADRVSSVLAVSAALARSRDRERAQTQRADALNEECQRLEHIIQGTADRH
                     RAISERLARGARSSAFGFAARNALLELERAAKNRTLIRVIVPQGSDGVAYAAHFHLAS
                     PRAGGPFIVVDAATPENQTPERFEDEANSPFTLADGGTLVWLNVLALSIPQQDTLSLA
                     LSRRLAYQPRSSILPPGVLMLLPKPAAELVERGMLSGSLARWFDEAEIQLPRLSDRSE
                     DFRSIALDCLTRECLAQGRPPIGLESSALRTLIEHDWARNEAELGSVLGRALALASGP
                     TLTLQDLEASGFQPTIRPAEPPSPLPGPARRRTTRRPKGRI"
     gene            3044..4366
                     /locus_tag="VFQ61_26050"
     CDS             3044..4366
                     /locus_tag="VFQ61_26050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET9957998.1"
                     /translation="MKKNYVLDTNVLLHDPFAIFKFEDNALILPIYVIEEIDQFKRES
                     NERGRNARTVSRLLDGERAKKGSLADGVPIGDGGSLRVHVPDKRPKLEIALNPNSGDH
                     AILQTAIELRDASPEMPTIFVTMDVNLRIRADALGLVTEAYENQAVDAERLESGIVEV
                     VVSAAELDVFFEKLAIKPPEGAQLHANVSVVLRDDTAPRPRSALGRFNLESGEIRALK
                     TPRDGVVGVRARNKEQSFALDLLLDDNVKLVTILGKAGTGKTLLALAAGLKRTIEDGS
                     YSRLLVSRPVMPLGRDLGFLPGDVNEKLNPWMQPIYDNLEFLLAAGGGKRRGIRGYDD
                     LFQSGQVQVEPLTYIRGRSLPQQYVIVDEAQNLTPHELKTVVTRCGEGTKIILTGDPF
                     QIDNPYVDSATNGLSVTADRLRGEGIVGHILLSKGERSELANIAANKL"
     gene            4363..5481
                     /locus_tag="VFQ61_26055"
     CDS             4363..5481
                     /locus_tag="VFQ61_26055"
                     /inference="COORDINATES: protein motif:HMM:NF019626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine/phenylalanine carboxypeptidase
                     domain-containing protein"
                     /protein_id="HET9957999.1"
                     /translation="MTSLGLAAPLHLAAELSRAERTLLRAERSIRLIDRLIPLEFATE
                     RERLLCALTAGQAAEPRFRYAAAPEIARFAVELNLLAESLAQYPEPAHFYADRARELA
                     LEAELVVAIGSRRFVELAALRYRPERLHGKPEIDAWVDENLSASRAGQSTKYPETAEE
                     TQSTERIRTDDTRDPRSLYNLLLREVGASKWPIRVEVRRELWSVAAAGEGFVAIRAGA
                     LLTEREARRIVRHELMAHAVPRVLAVREGQGIFRVGARDAGEEEEGRALWLEEQGGYW
                     DGARPRVLALRHHAASLVRAGATLNELVDSLAGLGAALPAALDLSLRVLRGGGLCREL
                     MYVPAYLRVRAEFQRDPRVENVFQRGRASIAFARWLLG"
     gene            complement(5484..6395)
                     /locus_tag="VFQ61_26060"
     CDS             complement(5484..6395)
                     /locus_tag="VFQ61_26060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958000.1"
                     /translation="MRRVPLGASGFAPALAAAVLALRAGSAAACGTCGAGDPSLTAAG
                     TEQPFAGRQRATLELSYRTDRIGRDGFDRLDIREARATTTLAYAVNAQLFALLSVPTV
                     YRRVQEVNLAVHEQWSLGDIEARGKWFFFRDRPFQPRFLAALQLGFKFPTAPFRTGTT
                     APPWPLEAQTGTGSWDLLAGPALAVFAAPVSFYLSAQWAEPLYSRRELHPGRAVRSTL
                     AGQYQFASWVALRVAHDTRLEARTRETLGTDPNSGGFVAYAGGDVLLSLHPNWLLSLG
                     ARLPWFQGMHGAHREGPIFAVALTLDR"
     gene            complement(6407..7156)
                     /locus_tag="VFQ61_26065"
     CDS             complement(6407..7156)
                     /locus_tag="VFQ61_26065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958001.1"
                     /translation="MPDSLVRAALVSLAVQCACSTTQAGIEVEVWSEPLHRSTSEPLP
                     RSASEPLSSNLGYTVRLEELLWSSSGLALKSCEAAVSRSLQRWFLGTAHAHGAASPTR
                     LSTSSVESGLAENATWIGTLRPPADAYCGATYSFAPADSDALGLPRWPEMLGVTLYAR
                     GTYQAGRDEPRPFELATSASFNSALEGLTLDVRHEARQVTLRLVREPAALFDDLEFSD
                     LESSSTAPDVLAHTLLRNLGTSTRAVIHEEE"
     gene            complement(7236..8450)
                     /locus_tag="VFQ61_26070"
     CDS             complement(7236..8450)
                     /locus_tag="VFQ61_26070"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HET9958002.1"
                     /translation="MDTSFWLLISLACGLLVLGFVFRGKPSTAPAQSPGRARSEPQGS
                     QPGNKTSKPEKRTEGARSGNGAKRSKPRPMPQPLPGADDDDLEITLITAFPLAELQAQ
                     VAKEASISDLARPQPKVDVEKLQQTAEGETTRVELMYEDEAELEETTSPVARILVSAA
                     GDSDQGRKRPRNEDSLLVFPERSLFVVADGMGGHAGGQVASAVAVDTLRDAFEKGNFD
                     ARTECEAKVPRRGRELACAIQMANQAIYNMAAEKPELNQMGTTVVAARFSPNKQRVYI
                     GHVGDSRCYRLRGNVLRQLTTDHTMKTLGVKGPRAHELFQAVGVKPAISIDLIVDKPR
                     DNDIYLLCSDGLCKMIPDEEVRDMLLEESDLEAAVYGLIERANDRGGKDNITLILVKV
                     LSRVADELRGMR"
     gene            complement(8728..9708)
                     /locus_tag="VFQ61_26075"
     CDS             complement(8728..9708)
                     /locus_tag="VFQ61_26075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET9958003.1"
                     /translation="MQSLPMQYDVTVVICTRNREERILSAVSSVLANDNQRFSLLVID
                     QSDGDRTRRALDPVKGDTRLRIVSSSERGLSRARNQALRLAETELVLFTDDDCEVPSN
                     WVADFVSSLEGRPSVAVAFCPVVPGPHDESAGYVPNFECSGTRVLSSIADLSPQRGMG
                     AGFAVRRSAALELGAFDEELGAGGSFPAAEDCDIAVRALLNGYEVMETDATFVIHHGF
                     RSWLEGRALARRDWVGLGAAYSKPLRAGYWTFGPVLARELLVNVAWPPLRDILNLRRP
                     KGLGRIAYFASGIARGFMAPFDPRSVVFLGADQLQGADQLGAAEQLSAAE"
     gene            10144..10395
                     /locus_tag="VFQ61_26080"
     CDS             10144..10395
                     /locus_tag="VFQ61_26080"
                     /inference="COORDINATES: protein motif:HMM:NF005480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9958004.1"
                     /translation="MIEDIREILQQHARLGVDANSLSPDSDLYDAGLTSHATVNLMLA
                     LEDRFQVEFPDELMDRGSFASISSIRSAIEQIRSRAISA"
     gene            10441..11355
                     /locus_tag="VFQ61_26085"
     CDS             10441..11355
                     /locus_tag="VFQ61_26085"
                     /EC_number="6.2.1.n2"
                     /inference="COORDINATES: protein motif:HMM:NF005479.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--[acyl-carrier-protein] ligase"
                     /protein_id="HET9958005.1"
                     /translation="MGDMESQVSFLNELFEAQILIPSGVPGLYGRSATFENILNAFDA
                     FVSRAGAPDRPSLVRFPPLIDRATFQRSGYMKNMPQLAGTVHSFMGGQPEHVQLIQEL
                     ESGADWSHHQKMTDVVLTPAACYPLYPSQSGEIGDTPRTFDVQSYCFRHEPSQDPARM
                     QVFRMREFVQLGSAEAVRSFRSSWFDRGQELFARVGLEVHAAPANDPFFGRTGRMLAA
                     NQREQELKYEMVVPIASEAPTAIMSLNYHQDLFGSTFGIRRLGNPAHTACVGFGLERI
                     TLALLKKHGLQPATWPEAVRTTLFPNGL"
     gene            complement(11563..13350)
                     /locus_tag="VFQ61_26090"
     CDS             complement(11563..13350)
                     /locus_tag="VFQ61_26090"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9958006.1"
                     /translation="MLCCPLGFELLSSYRTYQRLADALSLEGFPVLRFDYPGTGNSLP
                     AAQTTDEVSEWLAGVRDAIEEVRELSGAERVSLAGLRFGATLAALASEQLAWVESLVL
                     WAPCVSGRGYARELQAASRLKPSEVPLTDGIEAYGFAYSKRALDAISAVDLRKLDVSG
                     KHVLLLERDDVASTSDLAKSWTSSASSLEARSGLGYAAMMVEPHNSVLPRETIANVVD
                     WLAKRHPERELSAESAEPQVTTESGYADRDYFETPLTFGQRSHLFGILTQPAAQRGDH
                     ERMKRPVLLLLSAGADHSIGPHRMHVDVARAAVEAGYTCFRLDASGIGDSRIVDQRRR
                     HIYEMAAVEDVRAAMDAITERTGAEEFILLGLCSGAFVAFQTTQVDSRVVGEILINPR
                     RLTLGADASMHDEFKLYHKSSSYYVNALLSREHWARFLRGKLSIRGGVRYLTTLAAVR
                     AQKFLNAKLRGLPEQDEVCAGFQQICQRGTRTLVLAGLQDNGVDYLEYHIGHRGSKLA
                     RFEGFRVQLLAGSDHAFSTVHSQRMLRECILEELRAHRFRDMNVRSGARERKSAVRLK
                     TTERLPDAAASRGSASGGSASRGSAREFQ"
     gene            complement(13718..14494)
                     /gene="xth"
                     /locus_tag="VFQ61_26095"
     CDS             complement(13718..14494)
                     /gene="xth"
                     /locus_tag="VFQ61_26095"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET9958007.1"
                     /translation="MRLSSWNVNSIRVREPQLLDWLGRTRPDVVCLQETKVVDDDFPT
                     DDLMRLGYAVAMSGQPTYNGVAILSRLPLKDVRIGLAGEGPDAERRAISASVGGLRVM
                     SVYVPNGKSVSSPSFRDKLAWLERLRLTLDALEDPERPLVVCGDFNIATDERDVYAPE
                     QFRGKLHFHPEEHRALARVKDFGLVDAFRHKHDEAGAYTWWDYRTGGFRKNEGLRIDY
                     TFVTSNLLPRLSSASIDRETRALDKPSDHAPVSIELEDQL"
     gene            14686..>16213
                     /locus_tag="VFQ61_26100"
     CDS             14686..>16213
                     /locus_tag="VFQ61_26100"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HET9958008.1"
                     /translation="MRHWLCVSKTLLFMSLAASHALAQPGTQPPNDADDGPHEGSGAI
                     PGGSSGAVVRDAPEPEAPAKPVVVMPKLVHFESAPYPPEAEAQGLQGDVVLKLTIDRT
                     GAVTQAEVVQGLGHGFDGAAQAAALKFTFEPATRNGQPIGVQILYKYSFTLTPKVEAA
                     PPPPTTGELTGKLNISGSDAPLAGVEVVLTLPNGTEQRVTTDAEGRFRLPGALPGTYR
                     VRVATPGFRELQSDEQVTVGEATDLVYRLFPETTEDEIVIEGERPAREVTRRTIERRE
                     IERIPGTSGDALRSIESLPGVGRSPGLAGLLIVRGSSPQDTDIYVDGSVTPLAYHFGG
                     LTSVIPTELLERIDFYPGNFSARFGRVQGGIVDVGMRSPDTRCNEPYGKPGQNKGCYH
                     GLLQFDLIDGRFLFQGPVASSKHWSFALAGRRSWLDTWLKPVLESAGTSVSTAPVYYD
                     YQAILEYKRQKQRFSLRFFGSDDRLKVLVSDPFAQDPGFGGNLSFGTAYYRAQALYSN
                     D"
BASE COUNT         2956 a         5247 c         5127 g         2883 t
ORIGIN      
        1 cgccagcatc tactctgccg ccgctctcgt tgagcgctcc tccttgggtg cgcccgccca
       61 tcagggggtt atggctcgag cctccgccgg tgccgcccga atgagcgttg gcatcgccgg
      121 cggagctgtg cgctacacca ccgctagtca ttacggagga gctcccgcca gcggaaacgc
      181 cgcccgccgt ggagtgtccg gctcccccgc ttgcgactcc ggatgtcgtg ctcttcggag
      241 ctgcagcacc agcacccccc gtgcttacgc gattgcccgc agaaccaccg gtggcagtcg
      301 tggaatcgac ctcatccgac gagcagccgt ggagcgccgt ggcgccgagc aacgaagacc
      361 aaacgagcag agatcgcaca cccagggaga atttcaccac ttgctccaag cccaatcaga
      421 gccgcaacgt acccagagcc cagcgccctg gcaaccgtca ccgccagcag ctcgagagcg
      481 cccctcggcg gcggagggcg gcgcgtaaat gcgccttgct ccattcaggc tcagatgcgc
      541 cccttgggtc tgcgtgtcgt tcttctgcgg gccgggcctg gcagagggct aggcggctct
      601 gccggacgta tcgttggttg gaaacccgaa gcttccaggt cctgcagggt gagggtaggg
      661 cccgaagcga gcgcaagggc acgaccaagc acgctgccga gttccgcttc attgcgtgcc
      721 cagtcgtgct cgatcaacgt gcggagcgcc gacgactcga gtccgatcgg cgggcgcccc
      781 tgggccaggc actcgcgggt gagacaatcc agcgcaatcg agcgaaagtc ttcggaacga
      841 tcggacaagc gcggcagctg aatttccgct tcgtcgaacc agcgtgccag gctgccgctc
      901 aacattcctc gctccactag ctctgcggcg ggcttcggca gcagcatgag cacgcccgga
      961 ggcaggatcg aggagcgagg ctgataggcg agtcggcgag acaacgcgag agacaacgtg
     1021 tcttgctgcg ggatggagag cgccagcacg ttgagccaaa ccaaggttcc gccgtcggcg
     1081 agggtgaaag gcgagttggc ttcgtcctcg aaacgctcgg gagtttggtt ttcgggggtt
     1141 gccgcgtcca ccacgatgaa tggaccgcct gcgcgcgggc tcgccaagtg aaaatgggcg
     1201 gcgtaggcca ctccgtcgga accttgcggc acgatcactc gaattagggt gcggttcttg
     1261 gcggcgcgtt ccaattcgag cagcgcgttg cgcgcggcga agccgaaggc ggacgagcga
     1321 gccccgcgag ccaagcgttc actgattgcc cggtggcggt cggccgtacc ctgaatgatg
     1381 tgctcgagcc gctgacattc ttcgttgaga gcatcggcgc gctgcgtttg ggcgcgctcg
     1441 cgatcccgag aacgggccag ggcggcgctc accgccaaca ccgagctgac tcgatctgcc
     1501 aagagccgcg ccgcgcgcgc ctcttcgagc gtgagggggg agctccgacc cgcccgaggc
     1561 agcaacacga agcccaccgg gccatcgtcg tccaccacga tggtggcaga gaatgccttg
     1621 cgcgactcga gccacgccag cactccgcgc acttcagccc gcttcacctc gagggctttc
     1681 aagatatctg cccgcaacgt gcgctcgggc tcctgcatcc cgagctcgta aatgcgctcc
     1741 ggcgcctccg atcgcgttac cgtgaggtaa ccggccacat ccacgctgag cacgtccggc
     1801 ggatccttct gccagatttc ggcgccctga gtgtccgagc cgctgagctt gccgagcgcg
     1861 agcaacgcgg ctctcaaagc ggcttgcggc tccggttgca atgcgtcacg cgcggctcga
     1921 tccaaggcca cgagccaacg cgactgttcc ggcccgagcg ggcgcgctac ggcgtgcccg
     1981 gccaagccaa cgctaaccgc gaatatgccg ctgaccagcg ccaccgctgg cgcctgctcg
     2041 ggcatgagct tgctcaaagt cgtggcggca acgagcaacg gtgcgcccaa caacgtgagc
     2101 gcgagcacgc cccgcaacat gcgcgtgact agcgtggctt cgcgcgtaat ggacgcccag
     2161 gcacaggcgc tcgacactac gaccacggtc gcgggcagca ggcggtcggg cgcggccacg
     2221 tccaaccacg ccgcgggcac cgccaccgta aagcccgcaa tgcacagcgc aaacgccgag
     2281 cgtacccggt caccggtgcc gagctccaag cgccgcaaga agcgcaaacg cagggtcgcg
     2341 gccaccatca cgagcagcga gccaatgccc gcgctgctgg tcacgtagtc gatcaccagc
     2401 gggtcgaagc gcagcgcgct cggcggcaaa atcgcgtaac ccgcagggac ggccgtcgcg
     2461 aggccccaca aaaagccggt gaacaacacc gcgtcgagcg aacgcgccgc gcgatgtacg
     2521 tcgagcaaac ccgccaaggc cggcagacga ctcaacgcta ggagcgaccc gacggcgctc
     2581 gcccccgcgc ccaagttggc cagcgcgtcg agcgcgggct gagcactctc gcccgtacgc
     2641 gccgcgatca gcagcgccgc gccgaacccc gtgagccgca cgccggagcg ggcctccaga
     2701 gcgtaacgat catcgccggg aacctgctcg gtacgtcgga aaacctccga caacaagagt
     2761 ggtagtgcga ccagcgcgag ccacggcagc ggcccactcg tggtgcgcgc ggcggccagg
     2821 ctcgcgtagg ccaaaatggc cgccataatc cagtaatttc gaccgagctg cggcacgccg
     2881 gctgcttata ctcccgtgtg cgggtccatt ctcgcgtgaa aaagagccat cccccatttt
     2941 tgccgattgc cgagcgctgg cggcagcggt atccgccctg gtccgcgtgc cgggacccgc
     3001 gtttcggtgt gccggtgccg gagtccctgg agcgtcaccc ccgatgaaga agaactacgt
     3061 cctcgatacg aacgtcctgc ttcacgaccc tttcgcgatc ttcaagttcg aggacaatgc
     3121 gttgattctc ccgatctacg tgatcgagga gatcgatcaa ttcaagcgcg aatcgaacga
     3181 gcgcgggcgc aacgcacgca cggtatctcg cctgttggat ggcgaacgcg cgaagaaagg
     3241 atcgcttgcg gacggggtcc caatcgggga cggcggatcg ctgcgggtcc acgtgccaga
     3301 caagcgcccg aagctggaga tcgcgctgaa cccgaactcg ggcgaccacg cgatcctgca
     3361 gaccgccatc gagttgcggg atgcgtcgcc ggagatgccg accatcttcg tgacgatgga
     3421 cgtgaacctg cgcattcgcg ccgatgcctt ggggctcgtc acggaggcgt acgagaacca
     3481 agccgtggac gcggagcgtc tggagagcgg catcgtggaa gtggtggtga gcgccgccga
     3541 gctcgacgtc ttcttcgaga agctcgcaat caagccgccc gaaggcgcgc agctgcacgc
     3601 caacgtgagc gtggtgctgc gcgacgacac cgcgccgcgc ccgcgctcgg cgctcgggcg
     3661 gttcaacctc gaatcgggtg aaatccgcgc gctgaaaacc ccgcgcgacg gcgtggttgg
     3721 tgtgcgtgct cgcaacaagg agcaaagctt cgcgctcgat ctgctgctcg atgacaacgt
     3781 gaagttggtc acgattctcg gtaaggcggg cacgggcaag accttgctcg cgctggccgc
     3841 cggcctgaag cgcacgatcg aagacgggtc ttattcgcgc ttgctcgtga gccgccccgt
     3901 gatgccattg gggcgtgatc tcggcttctt gccgggggac gtgaacgaga agctgaaccc
     3961 gtggatgcag ccgatctacg acaacctcga gttcttgctc gcggcgggtg gcggcaagcg
     4021 tcgaggcatt cgcggctacg atgacctgtt tcaaagtggt caggttcagg tcgaaccgct
     4081 cacttacatt cgtggtcgca gcctgccgca acaatacgta atcgtggacg aggcccaaaa
     4141 cctaacccca cacgaactga aaaccgtggt cacgcgctgt ggcgagggca cgaagatcat
     4201 cctcacgggt gacccattcc agatcgacaa cccttacgtg gatagcgcga ccaatggcct
     4261 gagtgtcacg gccgatcggc tgcgcggcga gggcatcgtg ggtcacattt tgctcagcaa
     4321 gggggagcgg agcgagctcg ccaacatcgc agccaacaag ctgtgacgag cctcgggctg
     4381 gcggcgccat tgcacctagc cgccgaactc agccgcgcgg agcgcactct gctccgcgcg
     4441 gagcgttcga ttcggctaat cgatcgcctc attcccttgg aattcgcgac tgagcgggaa
     4501 cgcctgctct gcgcgctgac ggcggggcaa gcggcagagc cgcgctttcg ctatgcggcc
     4561 gcgcccgaga tcgcgcgctt cgccgtggaa ttgaacctgc tcgcggagtc gctcgcgcaa
     4621 tatccagagc cggctcactt ctatgcggat cgggcccggg agctcgccct cgaggcggag
     4681 ctcgtggttg cgattggctc gcgccgcttc gtggagctcg cggctctacg ctatcgtccc
     4741 gagcgccttc acggaaaacc cgagatcgac gcgtgggtag acgagaacct ctcggcgtca
     4801 cgcgcggggc agagcacgaa gtacccagag accgcggaag aaactcaatc caccgagcgc
     4861 atccgaacgg atgatacgcg cgacccgcgt agcctttaca acctgctgtt gcgcgaagtt
     4921 ggtgcaagta agtggccgat ccgggtcgag gtgcggcgcg aactttggtc cgttgccgcc
     4981 gcgggtgagg gtttcgtcgc gatccgcgcc ggagccctgc tcacggagcg agaagcgcga
     5041 cgcatcgttc gccacgagct catggcgcac gccgtgccgc gcgtgctggc cgtgcgcgaa
     5101 ggccagggca tctttcgcgt gggtgctcgc gacgcggggg aagaggagga agggcgtgcg
     5161 ctctggttgg aggagcaggg gggctactgg gacggcgcgc gtcctcgagt gctggccttg
     5221 cgtcaccatg cggcgtcgtt ggttcgggcg ggcgcgactt tgaatgagct ggtcgacagc
     5281 ctggcgggcc tcggcgcggc gctcccggcg gctttggact tgtcgctccg cgtgctgcgc
     5341 gggggtgggt tgtgtcgcga gctgatgtac gtgcccgctt acctgcgggt tcgtgccgaa
     5401 tttcagcgcg atccgcgcgt cgagaacgtg tttcagcgag gccgcgcgtc gattgcattc
     5461 gcgcgctggc tgctcggcta acttcaacga tccaacgtga gggcgacggc aaagatagga
     5521 ccctcacggt gggcgccgtg catgccctga aaccaaggca agcgcgctcc gagcgagagc
     5581 aaccaattgg ggtgcagact caaaagcacg tcccctccgg catacgccac gaagccgccc
     5641 gagttcgggt ccgtgcccag cgtctcccgg gtgcgcgctt cgagacgcgt gtcgtgggcg
     5701 acgcgcagcg cgacccaact cgcaaactga tactgcccgg cgagagtgct gcgtacggcg
     5761 cggcccgggt gaagctcccg gcgtgagtag agcggctctg cccactgcgc gctgagatag
     5821 aacgataccg gtgctgcgaa caccgctagt gccggtccgg cgagcaggtc ccatgatccc
     5881 gtccccgttt gggcttctag cggccacggc ggggcggtcg tgcccgttcg aaagggtgca
     5941 gtggggaact tgaacccgag ctgcagggcc gccagaaagc gcggctggaa cgggcgatca
     6001 cggaagaaga accatttgcc gcgagcctcg atgtccccga ggctccattg ctcgtgcacc
     6061 gccaagttca cctcctgcac tcgacgatac acggtgggca cggagagcaa cgcgaacagt
     6121 tgggcgttca cggcgtaagc caaggtggtg gtggccctcg cttcgcgaat atccagccga
     6181 tcgaagccgt cgcgcccgat tcgatccgtt cggtacgaga gctcgagcgt ggcgcgctgc
     6241 cgccccgcga acggttgctc cgtgcccgct gcggtcaagc tcggatcacc ggccccgcac
     6301 gtaccgcaag ccgccgcgga tcccgcgcgt aacgccagta ctgccgccgc tagcgcagga
     6361 gcaaagcccg acgctcccag gggtacacgc ctcatgccgc gcaggctcac tcttcctcgt
     6421 ggatgacggc ccgcgtacta gtgcccaagt tccgaagcag ggtgtgcgcc agcacgtccg
     6481 gtgcggtcga cgaagactcg aggtcagaaa actctagatc atcgaagagc gcggcgggct
     6541 cccgcacgag gcgcagcgtg acttgccgag cttcatggcg tacgtcgagc gtgaggcctt
     6601 cgagggcaga attgaaagac gcactcgtag cgagctcgaa gggccgtggt tcgtcccttc
     6661 ccgcctggta agtgcctcgg gcgtacagcg ttacaccgag catctctggc cagcgtggca
     6721 gcccgagcgc gtctgaatca gcgggcgcaa acgagtacgt cgctccacaa taggcatccg
     6781 cgggcggcct caaggtgcca atccaggtag cgttctcggc gagcccgctc tccaccgaac
     6841 tcgtggagag ccgcgtgggc gaggcggcgc catgtgcgtg agccgtgccc aagaaccatc
     6901 gctggaggct acggctgacg gctgcttcac aggacttcaa ggcgagcccg gagctcgacc
     6961 agagcagctc ctcgagccgc accgtataac cgaggttcga gctcagcggt tcgctcgcgc
     7021 tccgcggcag tggttcgctc gtgctccgat gcagcggttc ggaccatacc tcgacttcga
     7081 ttccggcctg cgtcgtactg cacgcgcact gcaccgcaag cgaaacgagg gctgcacgca
     7141 cgagagaatc aggcaagcgg ctggctcata gcgcctctcg ccgtgctcgc ccagcggcaa
     7201 ttggtcgcac ccgctcggga tatcagggtg cgctctcagc gcataccgcg gagctcgtcg
     7261 gccacgcggg acagaacctt caccagaatc aacgtgatat tgtctttgcc gccgcgatcg
     7321 ttggcgcgtt caatcaagcc gtacactgct gcttccaggt ccgattcctc gagcaacatg
     7381 tcccggactt cctcgtccgg gatcattttg cagagaccgt ccgagcacaa caggtagata
     7441 tcgttgtcgc gcggcttgtc cacgatcaga tcgatgctga tggcgggttt cacgccgacg
     7501 gcttggaaca gctcgtgcgc gcgcgggccc ttcacgccca acgtcttcat cgtgtggtcc
     7561 gtggtcagtt gccgcagcac gttcccgcgc aggcgataac aacggctatc gccgacgtgg
     7621 ccgatgtaga cgcgctgctt gttgggcgag aaacgagccg cgaccacggt cgtgcccatt
     7681 tgattgagct cgggcttctc ggcggccatg ttgtagatag cctggttggc catttgaatc
     7741 gcgcaggcca gctcgcgtcc gcggcgcggg accttggcct cgcactcggt gcgcgcgtcg
     7801 aagttgccct tttcgaaagc gtcgcgcagc gtgtcgactg ccaccgcgct cgcgacctgc
     7861 ccacccgcgt gcccgcccat gccgtccgcg accacgaaca gcgaacgctc tgggaaaacc
     7921 aacaagctat cttcgttgcg gggccgcttg cggccttgat cgctgtctcc cgcggcgctc
     7981 accaagatgc gcgccactgg ggatgtcgtc tcttcgagct cggcttcgtc ctcgtacatc
     8041 agctcgacgc gcgtggtctc tccctccgcg gtctgttgta gcttttcgac atccactttg
     8101 ggctgaggcc gagccaagtc cgaaatgctg gcttctttcg cgacctgggc ctgcagttcc
     8161 gccaacggga acgccgtgat cagcgtgatt tcgagatcgt cgtcgtccgc cccgggaagt
     8221 ggctgtggca ttgggcgcgg cttgctgcgc tttgcgccgt tgcccgagcg agctccctcg
     8281 gtgcgcttct ccggtttgct agtcttgttc cccggctggc ttccctgcgg ttcggacctg
     8341 gcccgtcctg ggctttgtgc tggcgccgtg ctcggcttac cccggaagac gaagcccaac
     8401 acgagtagcc cacacgccaa ggaaatcaga agccagaacg aagtgtccac agcctacttc
     8461 ttacaggctt ggagctcgcc ggtcgaccgc tggctgcatg gacatgcgcg ccatcgcgtt
     8521 tagatctgcg gacactcgag tatctcccga cttggcgggc ttttctggcg tgggtagggg
     8581 cgcgggcgcg tacggtgttg taggagcccc gagcccctgt cgactactct gtttagcatc
     8641 cgccggttcg gcatccgccg gtttgctgtc cgcgattacc gcgcggcgcg ggtgttggtg
     8701 ggagggtatc gggggttctg agcgtcgtta ctcggccgcg gacaattgct cagctgcgcc
     8761 tagctgatcg gctccttgga gctgatccgc gccgagaaac acaaccgatc gaggatcgaa
     8821 tggcgccatg aagccgcgcg cgattccgct cgcgaaatac gcaatcctac ccagcccttt
     8881 cggccgcctc agattgagga tgtcgcgcaa agggggccag gccacgttca cgagtagctc
     8941 acgcgccaac accggaccaa aggtccaata gcccgcgcgc agcggtttgg aatacgccgc
     9001 gccgagccca acccagtccc gccgtgccag cgcgcgtcct tcgagccagc tccgaaatcc
     9061 gtggtgaatc acgaaagtcg catcggtttc catgacctca taaccgttca agagggctcg
     9121 aacggcaatg tcgcagtctt cggctgccgg aaaacttccg ccggcgccga gctcctcgtc
     9181 gaacgcgccg agctcgagcg ccgccgagcg ccgtaccgcg aaaccggcgc ccatgcctcg
     9241 ctgcggggat aggtccgcga tgctcgacag tacacgcgtg cccgagcact cgaaattggg
     9301 tacgtacccc gcgctctcgt cgtggggacc tggaacgact gggcaaaacg cgacggcgac
     9361 gctgggccgc ccctccaggc tcgagacgaa gtcagccacc caattgctgg gcacttcgca
     9421 gtcatcgtca gtaaacaaca cgagctctgt ttcggcgagg cgcaaggcct gattgcgcgc
     9481 gcgtgacagc cctctttcgc tgctggatac gatacgcagc cgtgtgtcgc ccttcaccgg
     9541 gtcgagcgcg cgccgagttc gatcgccatc agactggtcg atcacgagca gactgaatct
     9601 ttgattgtca ttggctaaca ccgaagaaac ggcgctcaga atcctctctt cgcgattccg
     9661 agtacagatt acgaccgtca catcgtactg catgggcaga ctctgcatgg gctatctccg
     9721 ctcgccttgg gtcctcgtcc aggcgcgcgc tctggactgc cggtttcatg ccaacccgcc
     9781 ggattagcgg tacgcgcgcc cgcgctgggt ccccggttcg tcggttcggt cgtcgcgcct
     9841 gagcgtcgca tcgagaggat cgagcaaact catgagcgaa cgaggcggta cggtgaggcg
     9901 tcgcgcgcgc aggcaaacaa gaattgcacg caagagcgcc gcgcgatgtc ggcagtgaca
     9961 cacgccgctg cgtccaatgc cctagattga gccatttctc accgtgtcgt cgctgcgacg
    10021 cgagcttgac acggtttgtg cttaccgcag ctgcggcgcc gtaccccgtg cggtgttccg
    10081 gaatcgatgc gacgcaaata tcgagctctc gcggaacgcg cgagctcttg aggctgatcg
    10141 accatgatcg aggatattcg agagatactg cagcagcatg ctcgcttggg cgtagacgcg
    10201 aactccctgt cacccgacag tgacttgtac gacgcgggtt tgacctcgca cgcgacggtg
    10261 aatctgatgc tggcgctgga ggacagattt caagtcgagt ttccggatga gctcatggat
    10321 cgcgggtctt tcgcgagcat ttcctcgatt cgaagcgcga tcgaacagat tcggtcgaga
    10381 gcgatttctg cgtgacgttt ggacggcgcg cactccagga tagtgagtaa ggccaaaaag
    10441 gtgggtgata tggaatcaca agtttccttc ctgaacgaac tgttcgaagc ccaaatcttg
    10501 atccccagtg gcgtgccggg gctatacggc cgaagcgcaa ccttcgagaa catcttgaat
    10561 gcgttcgacg ccttcgtgtc gcgtgccggt gcgcccgatc ggcccagcct agttcgattt
    10621 cctccgctga tcgaccgcgc gactttccag cgcagcggat acatgaagaa catgccgcag
    10681 ctcgctggca cggtgcacag cttcatgggg gggcagcccg aacacgtgca gctgattcag
    10741 gagctggaga gtggtgcgga ttggtcacat catcagaaga tgacggacgt cgtgctcacg
    10801 ccggcggctt gctatccctt gtacccgagt caaagcgggg agatcgggga caccccgcgc
    10861 accttcgatg tccagtcgta ttgctttcgt cacgagccct cccaagaccc agcgcggatg
    10921 caggtatttc gaatgcgaga gttcgttcaa ttgggatcgg ccgaggcggt gcgcagcttc
    10981 cggtccagct ggttcgatcg cgggcaagag ctgtttgcca gagttggcct cgaggttcac
    11041 gccgcaccgg caaatgatcc ctttttcggg cgaaccggcc gcatgctcgc cgcgaatcaa
    11101 cgcgaacaag aactgaaata cgaaatggtg gtgccaatcg cgagcgaagc tccgactgcg
    11161 attatgtcgc tgaattacca tcaagatctg ttcggcagta ccttcggaat tcggcgactg
    11221 ggcaaccccg ctcacactgc ttgtgtaggg ttcgggctgg agcgcatcac actcgcgctc
    11281 ttgaagaagc atgggctcca acctgcgacg tggcccgagg cggttcgaac aactctattc
    11341 ccgaacggcc tgtaaccaag cccaaggcct ttgggcaacg ttcccgggcc gctcgtaaga
    11401 agatcgtgat tcgatcgaga gaccgcggcc tggacgcggt ctcgatcgaa tcacgagtct
    11461 ggatcgactc ttaccgatgc gggctaccga ttcagtcggc gcattcggca ccacacccgc
    11521 ggtactcggt cccaacacac gggcaacagg ccgtgactcg agtcactgga actcgcgcgc
    11581 actgccgcga gaggcactgc ccccggaggc actgccgcga gaggccgccg catccggcag
    11641 tcgctcagtg gtcttcagtc gtaccgcgct ctttcgctct cgcgcccccg atctaacatt
    11701 catgtctcga aatcggtgcg cccgaagctc ctccaagatg cattcgcgga gcattcgctg
    11761 actatgcacc gtactgaatg catggtcgct accggctagg agctgcacgc ggaagccctc
    11821 gaagcgagcg agcttggagc cgcggtgccc aatgtggtac tcgagataat cgaccccgtt
    11881 gtcttggagt cccgcgagca ccagcgtgcg cgtcccacgc tgacagatct gctgaaaacc
    11941 agcgcacacc tcgtcctgtt ccggaaggcc gcgcagcttc gcgttcagaa acttttgcgc
    12001 tctcaccgcg gccagcgtcg tcaggtagcg cactccgccg cgaatcgata gcttgccgcg
    12061 caggaagcgg gcccagtgtt cgcggctcag gagcgcgttc acgtagtagg agctggactt
    12121 gtggtagagc ttgaactcgt catgcatgga tgcatccgcg cccaacgtga gtcggcgcgg
    12181 attgatcagg atctctccca ctacccgtga gtctacttga gtggtctgga acgcaacgaa
    12241 ggctcccgag cacaacccaa gcaggatgaa ctcttcggcc cccgttcgtt ccgtaatggc
    12301 gtccattgcc gcccgcacgt cctccaccgc ggccatttcg taaatgtgtc gccgccgctg
    12361 gtccacgatt cggctgtcac cgatgccaga tgcatctagt cggaagcagg tgtacccggc
    12421 ttcgacggca gcgcgcgcaa cgtccacatg catacggtgc gggccaatcg aatgatcggc
    12481 tccagcgctc aggagaagca ggaccgggcg cttcatgcgc tcgtggtcgc cgcgttgcgc
    12541 cgcgggttgg gtcaggatcc cgaacagatg cgagcgctga ccaaacgtga gcggcgtctc
    12601 gaagtagtct cgatctgcgt aaccggactc ggtcgtgacc tggggctcgg cactttcggc
    12661 agagagttct cgctcggggt gtcgcttcgc cagccagtcg actacgttcg cgatcgtttc
    12721 ccgcggtagc acgctgttgt gcggctcgac catcatcgcc gcgtagccga gtccactccg
    12781 ggcttccagg ctgctcgccg acgacgtcca cgacttggct agatcgctag tgctcgcaac
    12841 gtcatcccgc tccaggagca acacgtgttt gcctgaaacg tcgagcttcc ggaggtcgac
    12901 tgccgagatg gcgtccaggg cgcgcttcga ataggcaaaa ccgtacgcct cgatgccatc
    12961 ggtcaacgga acctcactcg gcttgagacg actggcggcc tgcagctcac gagcatagcc
    13021 gcgaccgctg acgcatggcg cccagagcac caggctttcg acccacgcga gctgctccga
    13081 ggccagcgcg gccagagttg cgccgaaccg cagcccggcg aggctaaccc gttcggcgcc
    13141 agacagctcc cgtacttctt cgatcgcgtc ccggactcca gcgagccact cgctcacttc
    13201 atccgtcgtc tgtgcagcgg gaagtgagtt gcccgtacct ggataatcga agcgcagcac
    13261 gggaaagcct tcgaggctca acgcgtctgc caaacgctga tacgtacggt acgaactcaa
    13321 gagctcgaac ccgagcgggc aacacaacac gactgcggtc ttgcgagctg ggccgtgcgc
    13381 cgggtgatac cagccaaacg ccctgcgctc tttcccgaag taaagtggga tgggggacgg
    13441 ccctgggtgg attgaaccct catcccaagg taatggttgg ttttgctcag cgctctgggt
    13501 ttgctgcatc ctgagccgcg cctggtgcaa atggtgtgca aagccttttc attgcggcct
    13561 tgcgtgcgcc gtcgcttatc agagtcgccc tccaccgcag gagcgcctca gtgcgcgtga
    13621 tcgccccaca cagttagttt ccgagtgggt tccgaaaaca cccgattgtt tcgagctcag
    13681 ccgatcattc gactcgcgtg attggctgac gcggatacta aagttgatct tcgagctcga
    13741 tcgagacggg cgcgtgatcc gacggtttgt ccagcgctcg agtctctcga tctatcgacg
    13801 cactactcag acgcggcaaa aggttcgagg tcacgaacgt gtaatcgatc cgtaaacctt
    13861 cgttcttgcg aaagccgccc gtgcgatagt cccaccaggt gtacgctccg gcttcgtcgt
    13921 gcttgtgtcg gaacgcatcc accaagccga agtcttttac gcgcgccaaa gcgcggtgtt
    13981 cttccggatg gaagtgcagc ttgccgcgga actgctccgg cgcgtaaaca tcgcgttcgt
    14041 ccgtggcgat gttgaaatca ccacacacca ccagggggcg ctccggatct tccaaggcat
    14101 cgagcgttag tctcaaacgt tcgagccacg ccagcttgtc tcgaaatgag ggagagctga
    14161 cggacttgcc attgggaaca tagacgctca tcacgcgcag cccacccacg ctggcgctga
    14221 tcgcgcgccg ttccgcgtcg gggccctcgc cggccaagcc gatgcggacg tccttgagcg
    14281 gcagacggct gaggatcgcg acgccgttgt acgtgggctg tcccgacatt gcgacggcgt
    14341 acccgagccg catcaaatcg tcggtcggaa agtcgtcatc gacgaccttg gtctcctgca
    14401 gacacaccac gtccgggcgc gttctgccga gccagtcgag caattgaggt tcacgtactc
    14461 ggatcgaatt gacgttccag ctcgagagcc gcacggccct tcgcatacct tcgccgggcc
    14521 tcgcgcggca agctgaacct tccaaactga ctttcacagc gcttctaaag cgcgcgttca
    14581 gctcaccgct taccgctcag actcgaggga gactggcggc gcggccgtcc ggcgctgagc
    14641 aggcccttgt ggttcgaagc cttcgggact acgaaagtcc ccgctatgcg ccattggctg
    14701 tgcgtatcga agacgctgtt gttcatgagt ttggctgcaa gccacgcgtt ggcccagcca
    14761 ggaacgcagc cgcccaatga cgcggacgac gggcctcacg aagggagcgg cgccattcct
    14821 ggcggcagct ccggtgccgt cgttcgcgat gctcccgaac ccgaggcgcc cgccaagccg
    14881 gtggtggtca tgccgaagct cgtgcacttc gagagcgcgc cgtacccacc cgaagcggaa
    14941 gcccaaggct tacagggtga cgtggtgctc aagctcacca tcgaccgcac cggtgcggtc
    15001 actcaagcag aagtcgtgca gggcctgggt cacgggttcg acggagcggc ccaagccgcc
    15061 gcgctgaagt ttaccttcga gccagcgacc cgcaatggac agccgatcgg cgttcagatc
    15121 ctttacaagt acagcttcac gctcaccccc aaagtagagg cggcgccgcc gcccccgacc
    15181 acgggtgagc tcacgggcaa gctgaacatc tccggttcgg acgcaccgct cgcgggggtg
    15241 gaagtggtgc tcacgttgcc caacggtacc gagcaacgcg tgacgacgga cgccgaaggt
    15301 cgattccggt tgcccggggc gctgcccggc acctaccgag ttcgcgtcgc gacccccggt
    15361 tttcgggagt tgcagagcga cgagcaagtc acggtaggag aagctaccga tctggtttac
    15421 cgcctgttcc cggagaccac cgaggacgag atcgtgatcg agggcgagcg gcccgcgcgt
    15481 gaggtcacgc ggcgcaccat cgaacgccgg gaaatcgagc gcattccagg cacgagcggg
    15541 gatgcgctgc gttcgattga gagcctgccc ggggtcgggc gctctcccgg tttagccggt
    15601 ctgttgatcg tgcgcggctc ttcaccacaa gacaccgata tttacgtgga cgggtcggtt
    15661 acgccgttgg cgtatcactt tggcgggctg accagcgtca tccccaccga gctactcgag
    15721 cgaatcgact tttatcccgg aaacttcagc gcccgctttg gtcgggtaca aggcggcatc
    15781 gtcgatgtgg gaatgcgctc gccggatacc cgctgcaacg aaccgtacgg caagcccggt
    15841 caaaacaagg gctgctatca cggcttgctg cagttcgacc taatcgatgg tcgattcttg
    15901 tttcaaggcc cggtggcctc tagcaagcat tggagcttcg cgctggcggg gcgccgctcg
    15961 tggctcgata cctggctcaa gcccgtattg gagagcgcag gaacgagcgt gtccaccgcg
    16021 cccgtctact acgactacca agccatcctc gagtacaagc gccagaagca gcgcttcagc
    16081 ttgcgcttct tcggtagcga cgatcgcttg aaggtgttgg tgtcggaccc gttcgctcag
    16141 gacccgggtt tcggcggaaa cctgtcgttt ggaaccgcat actaccgtgc ccaggccctt
    16201 tactcgaacg atc
//
LOCUS       DASTKU010000743         8863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1037857, whole genome shotgun sequence.
ACCESSION   DASTKU010000743 DASTKU010000000
VERSION     DASTKU010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037857"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3371
                     /locus_tag="VFQ61_26105"
     CDS             <1..3371
                     /locus_tag="VFQ61_26105"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9958009.1"
                     /translation="QKALEAPVDAVVLVHGGIGSTLALDELAAEELASWLDLSAFVPV
                     DNVRSLGEITPRPKPASTQPLEDVRGAFGITPLSEDAHHLIRSLVRGRVAGEDSSPAK
                     LVKVAKGAGRLWNGLVGGLRAAWRRLFYPRRVRPPAHGLAVVQSSERPQGSWFSRLRA
                     GWSQFVARMLLLSRFAPLLGRRHAEYLTRMLEMFDSNRLDEALRHAIPLGGELDAALR
                     PPPLSPPSPRSTLAIAPQRTPATASLGLGEQLFDSLRQRYRRAYERLAAAGDIERAAF
                     VLAELLNSNEEAVSFLECNGRVRLAAEIAEARELAPGLVIRLWFLAGDHSRALRIALR
                     TGAFADAVVRLETTHPHHAHALRLRWADRLASGGAYAAAVDAVWNVPHARDLALDWLE
                     RAIQVGGVTGARMLARKAASVPSAFSEVRDRVLDSLQDPQTELTALRAFAQQLSTGDP
                     TPEARALARPTARRLLRDSADRETQQLVQRLLDLSGDATLRADVRASRSPTPKPNSRR
                     SQIQVRAFGCTHAGRMRGGNQDTCLLSFLDKHARALHGGGLDGNATTRGLVLGVFDGS
                     GGWESNTVGRMASQTIVNALQAEFAANTSALAAWARGLVFAMDRVNRQLFEAAQSPAH
                     RGTAASATVATFWDNKVLFAQLGDTRGHVLRGKDLIQVTRDHSLLNDFMSSMRDAGTP
                     MTQQQIEEFPHQSVITRALGITEEVVTDIWQAELEPGDDVLLCSDGLWSVIDADRIRT
                     TLIERHSPQAACDALLSMALEAGATDDISIVVARIEGKPPARAGGTLRHGPYVPPPPP
                     AEESASVLSLSSRPEPLLIQRRSGDRGAMQVLDAAELPDGQLLVALGEAGVWLLTREG
                     KVSTRFAEPADRLVISDHGDRAILVARRGEANRVARLDLLTRKVRYWCDARFDSSASD
                     FDGFTWFVSQGDTVYAIDATAEDWEHQWQLDERGAAVRALRRSSQTLSIWFVAPGTSG
                     EIWTFELPSFKLRQRQPLEHPDALVLLGAVSPGGKFAGWRAHPSKPPIAEAFIHRSWK
                     DLPGLALPHFLPPTPYLTQEWIALPALAQSGTTVHLVDASGGRERARLQLEGTAGSVG
                     VRIQGEHLCVFDACGRVIVLCLQTGTVLREHHVS"
     gene            3794..6994
                     /locus_tag="VFQ61_26110"
     CDS             3794..6994
                     /locus_tag="VFQ61_26110"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HET9958010.1"
                     /translation="MKTTVLHTCLSLLGASIVACSASRSEPQTDRSPGSSPGNVRLEL
                     NGTDVTPSTVDDTWNLAGSTPGWVGIDKLIDNDHTTYLWVGGGTDQITYQLARPTVIT
                     SYDIVAASESSGSPKDWRFEATQNGFDWMPLDTQTGQSFAPGTARTFALQSASPYIGV
                     RLVITASNVTADDGALRIAEFRVGGTLPSGVVPGAPTLQAVVDSTSARLSFSATNATG
                     YVLRRYSLDGGAKEISVSSSPYTDPDLIPGTPYVYTVQAVNGNVRGFPNTALARVVPP
                     VPPTALKDVTALFSDPPRAQYDLAPWVVGNVTDGHMSSKWYAGSNATSWLEQSANADT
                     VVTQYTLTSANDYPDRDPKTWELQGSVDGKQWTVLDRRQNQFFTGRLQTRRFTANTQR
                     LAFPLYRLAIVANHGAPDLQLTEWRLFGTTSATLAPPATPTGLAANALSSDQIQLTFD
                     RRTSRQNPETSLVVQRATDANFTQNVVERTAGPARDADAGPVFDYRALNLAPATEYFF
                     RVKAVNAAGSSAYTSTRSAKTANASAPASFVTGNWYPKANEQLDKVYSDANIVVYRDS
                     KVTDVPDANAIAWLTPALSEAWAYIKANYPVFSGPQLQVILGNQKGSASVTYAYSSQN
                     AYRNAIFSFSDKWSLADRHAWKFESLMHEMNHIVESNNNEMQNSPSYSAWGDSRWGEI
                     FMYDLFSHLASVPATGVEATHAQTLLDSWWEARDGVGAHWLQGWYYPLYTGALGNNAP
                     ANRGVAFLNKYFSLLAQYYPKFTNEYARTMNLGEYIHFCSAAAGLDLTTVARSVFRFD
                     RRPEVEAQLAQAQLDFPDVYALYGSNRAPGFTLDPIPVSGAAGSPLTGQTLAERAGDP
                     DLGATLTYSKQSGPTWLSVAADGSITGTPPTAGTYAAEVRVTDNGGLFDTATLTVVVS
                     GGACTPETDAAFCSRLSAACGALTGTDNCGAARSVASCGSCNSPLTCGGAGVANACGS
                     TGGTGPCSGLCTNPIVIPSAGFGSGALGTAATCHESKANLIGSNCSNFTARTFRINSA
                     LATCNGQPIPLPAKRNGGYCFQASAGEPAWATFSTY"
     gene            7104..7799
                     /locus_tag="VFQ61_26115"
     CDS             7104..7799
                     /locus_tag="VFQ61_26115"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958011.1"
                     /translation="MSTVHLVGGEKGGVGKSVVARVLAQYLVDRDLKFVGIDADRSHG
                     ALLRTYADFTQPADLEVFASADQIMDRALGSDRRVLVDLPAQSARALRRWMDAGGVVQ
                     FARDMDVKLVFWHVSDGGFDSVSELERAQATFEQSVTYVVVRNFGRSSDFAQLDESKA
                     LRDAVGRGGRVIDLPALDPATMYKVDRFGSSFWAAVHAQEGEFVLSPMERRRAELWLS
                     RAFHALDSLENVI"
     gene            7968..8615
                     /locus_tag="VFQ61_26120"
     CDS             7968..8615
                     /locus_tag="VFQ61_26120"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9958012.1"
                     /translation="MANLKFYMTPGSCSTGIHLLLEELELFFEAHVVNLLAGDHLKPA
                     FRALNPRGSVPVLVRTDGTALTDFISIARWLASTHPNHHLLPESDRETSRLLQVMEYV
                     VGVIHGEGFTRVFVSDRYAQDSLGKTSTEREGRQIVSRGFDRVETMIFGSPYISEAFS
                     IADAALFYVEFWADREGIPLPPKCERHYKAMLTRRAVRQVLGEEGYHSVLRRYPA"
BASE COUNT         1613 a         2875 c         2727 g         1648 t
ORIGIN      
        1 aacagaaagc attggaagcg ccggtcgacg cggtggtgct cgtgcacgga gggatcggga
       61 gcacactcgc gctggatgag cttgccgcag aggagcttgc gtcttggctc gatttatcgg
      121 cgttcgtacc cgtagacaac gttcgctctc tcggcgagat cacgcctcgc cccaagccag
      181 catcgactca gcctcttgaa gatgtccggg gcgcgttcgg gatcactccc ctttcggaag
      241 atgcccatca cctgatccgc tcgctggtgc gaggtcgtgt tgccggcgag gactcgagcc
      301 ctgcgaagct cgtgaaagtc gcgaagggcg cggggaggtt gtggaatggg ctcgtcggcg
      361 gcctgcgcgc ggcgtggagg cgcctcttct atccgcgtcg agtgaggcct ccggcgcacg
      421 ggcttgccgt tgttcagagt tccgagcgac ctcaggggtc ttggttctcc cgcttgaggg
      481 caggatggtc ccagttcgtt gcccggatgt tgcttttatc caggtttgcg cccctgcttg
      541 gccgtcgtca cgccgagtac ttgactcgca tgctcgagat gttcgattcg aaccggctcg
      601 acgaagccct gcgccatgca attccgctcg gcggggagct cgacgcggca cttcgccctc
      661 cgcccttgag cccgccatcg ccccgctcga ctctcgcgat tgcgccgcag cgtacgcccg
      721 ccacggcttc gctcgggctc ggcgaacaac tcttcgacag ccttcggcag cggtatcgac
      781 gcgcgtacga gcggctagcg gcggcaggcg acatcgagcg ggcggcgttc gtgttggccg
      841 agctcctcaa ttcgaatgaa gaagccgtct cgttcctcga atgcaacggc cgagtgcgcc
      901 tggccgcgga gatcgccgaa gcgcgcgagt tagcaccggg cctggtgatc cgactgtggt
      961 tcttggctgg ggatcatagc cgtgcgctgc gcatcgcact tcgaaccggc gccttcgcgg
     1021 atgccgtggt gcgcctcgag acgactcacc cgcatcatgc ccacgcgctg cgcttgcgct
     1081 gggcggaccg attggcgtcg ggcggcgcgt acgcggcggc cgtggatgca gtgtggaatg
     1141 ttccccatgc ccgagacctg gcactcgatt ggctggagcg ggccattcag gtgggcggag
     1201 tgaccggggc tcgcatgttg gcgcggaagg ccgcctcggt tccgtcggca ttttctgagg
     1261 tccgcgatcg cgtactggat tcgttgcagg acccgcagac cgagctgacg gctctgcggg
     1321 cctttgctca gcagctctcg actggtgatc cgacaccgga agcccgggct ctcgcgaggc
     1381 cgacggcgcg acggcttctc cgcgactcgg cggatcgtga gacccagcag ctcgtacagc
     1441 gattgctcga cctcagcggc gacgcaacgc tgcgagccga tgtgcgcgca tcacggtcgc
     1501 cgacgcccaa accgaactcc cgtcgaagtc agatccaagt gcgcgctttc ggctgcacgc
     1561 acgcgggtag aatgcgaggt ggcaatcaag acacctgcct cctatcgttc ctagacaagc
     1621 atgcgcgcgc tctccacgga ggcggcctgg acgggaatgc cacgacgaga gggctcgtgc
     1681 tcggggtctt cgacggtagc ggcggttggg agagcaacac cgtcggacgt atggcctccc
     1741 agacgatcgt gaacgcgctg caagccgagt ttgccgcgaa cacgagcgct ctcgccgcct
     1801 gggcacgcgg cctcgtgttc gcgatggatc gggtcaatcg tcagctgttc gaggccgccc
     1861 aatccccagc gcaccgcgga acggccgcga gcgcaacggt ggccacgttt tgggacaaca
     1921 aggttctttt cgcgcagctc ggggatacgc gaggccacgt gctgcgcggg aaagacctaa
     1981 tccaggttac gcgcgaccac tcgctgctga acgacttcat gtcgagtatg cgggatgcgg
     2041 gcacgccgat gactcagcag caaatcgagg agttcccgca ccaaagcgtc attacgcgag
     2101 ccctcggtat caccgaggag gtcgtgaccg acatatggca agccgaactc gagcctggag
     2161 atgacgtgct cctatgcagc gatggcctgt ggtcggtcat cgacgcggat cgcatccgca
     2221 ccacccttat cgagcgccac agcccgcaag ccgcctgtga tgcactcctg tcgatggcgc
     2281 tcgaagccgg ggcaacggac gacatctcta tcgtggtggc acgcatcgaa ggcaaaccgc
     2341 cggctcgagc cggtgggacc ctccggcatg gtccttacgt gcccccgcca cctcctgccg
     2401 aggagtccgc cagcgtcctt tcgctttctt ccaggcctga gccgttgctg atccagcgcc
     2461 gctccgggga ccgcggagca atgcaggttt tggatgccgc cgagctgccc gacgggcagc
     2521 tgttggtcgc gctcggcgag gccggagttt ggctcttgac tcgagagggc aaggtctcga
     2581 cccgatttgc cgaacccgcg gatcgactcg tgatctccga tcacggggat agagccattt
     2641 tggtcgcgcg ccgcggtgaa gcgaatcgag tcgcgcgatt ggacctgctc actcgaaagg
     2701 tgcgctactg gtgcgacgct cgttttgaca gctctgccag cgatttcgac ggcttcacct
     2761 ggttcgtatc ccagggtgac acggtctacg ccatcgacgc gaccgccgaa gattgggagc
     2821 accagtggca actcgacgag cgcggagccg cggtgcgcgc gctacgacgc tcatcgcaga
     2881 cgttgtctat ttggttcgtc gccccaggca cttctggcga gatctggacg ttcgagttgc
     2941 cctctttcaa actgcgccaa cgtcagccgc tcgaacaccc ggacgcgctc gtcctgttgg
     3001 gagccgtgtc gcccggcggc aagttcgcgg gttggcgagc gcaccccagc aagccgccca
     3061 tcgccgaggc cttcattcat cgcagctgga aggacttgcc cgggttggct ctcccgcact
     3121 tcctgccgcc taccccctac ttgacccagg aatggatcgc tctgcctgcg ctcgcccaga
     3181 gcggaacgac agttcacctg gtggacgcca gtggtggccg ggagcgggcg cggctgcaac
     3241 tcgagggaac tgcggggagc gtcggcgttc ggatccaggg ggagcacctc tgcgttttcg
     3301 acgcctgcgg ccgcgtcatc gtgctctgtt tgcagaccgg gacggtcctg cgcgagcatc
     3361 acgtctcttg agggttcgcg cggcgggaca cggggacgat agctgaatgg ctccgggtta
     3421 tcgagcacgg gccaaacggt cctcgctcgc atccgcggga cgcgctgacg atcgtcactt
     3481 cgcccctcga ttgcgctccc ccacttgaag gcacggtgca ggttgccgtc gcaacctcca
     3541 gttgcgcgac gtggttgcgc aacccggttg cgtaatgggc cggtgagttc cggagcaacg
     3601 tcgtgcgcgc accgaacaca cagcgcgggc gtcgtcaatt cacggtcgcc tcgggacttt
     3661 cgcgcgcaca cggcatcccg tccaacacta cacccgggcg ggttgtgcag cggcccgatc
     3721 tttgctgagc ggcagaagcc aaggcgggaa cgagacgctc ggggccggat gccagatcga
     3781 gagtaaccct tagatgaaaa caacagtcct gcacacgtgc ctatccctgc taggagcttc
     3841 cattgtcgcc tgcagcgcct cacgcagtga gccgcaaacg gatcgatctc caggttcttc
     3901 gcccggcaac gtccggctcg aactcaatgg aactgacgtc actccttcga cggtggacga
     3961 tacttggaat ctggccgggt cgacccccgg ctgggttggg attgataaac tcatcgacaa
     4021 cgatcacacg acttatctct gggtgggtgg cggcacggac caaatcactt accagctcgc
     4081 ccgaccgacc gtgatcacga gttatgatat cgtagctgcc agcgagtcat ccggctcccc
     4141 caaagactgg cgattcgaag ctacccagaa cggcttcgac tggatgccac tagacacgca
     4201 aaccggtcag agcttcgccc ccggtaccgc caggaccttt gcgctccaaa gcgcctcccc
     4261 ttacatcggg gtgcgactag tgatcacggc cagcaacgtc accgccgatg acggcgcgct
     4321 gcgcattgcc gagtttcgcg tgggtggcac gctcccgagc ggagtggtcc cgggcgcgcc
     4381 aacacttcag gccgtcgtcg acagcacgtc ggcgcgccta agcttcagcg ctaccaatgc
     4441 cacggggtat gttctgcggc gatactcctt ggatggaggt gcgaaggaga tctccgtctc
     4501 gagctcgccc tacaccgatc cggatctgat ccccggcaca ccgtacgttt ataccgtcca
     4561 agccgtgaat ggcaacgtgc gcggcttccc caacacggcg ctggcgcgag tggtgccccc
     4621 ggtcccgcca acggctctaa aagacgtcac ggctctcttc tcggatcccc ccagagccca
     4681 gtatgacttg gcgccgtggg tcgttggaaa cgtgaccgac ggtcacatga gcagtaagtg
     4741 gtacgctggt tccaacgcca cgagttggct cgagcagagc gccaacgcgg acacggtcgt
     4801 gacccagtac acgctcactt cggcgaacga ctatcccgat cgggacccca aaacctggga
     4861 gctgcagggt tccgtagatg gcaagcaatg gacggtgctc gaccgacgcc aaaatcaatt
     4921 cttcacgggg cgcttgcaga cacggcggtt caccgcgaac actcagaggc tcgcctttcc
     4981 cctctaccga ctggcgatcg tcgccaatca cggtgctccc gatctacagt tgacggaatg
     5041 gcgtctattt ggcaccacga gcgccacgct ggcgcccccg gccacgccga ccgggttagc
     5101 ggccaacgcg ctctcgagcg atcagatcca gctgacgttc gatcgtcgca cctcccggca
     5161 aaacccggag acgtcgctgg tcgtccagcg cgcgacggac gcaaacttca cccaaaacgt
     5221 ggtggagcga acggcgggac cagcccgaga tgccgacgct ggacccgtat tcgactaccg
     5281 cgcactgaat ctcgcccccg caacggagta tttctttcgg gtcaaagccg tgaatgcggc
     5341 gggatcgtcc gcgtacacca gcactcggtc cgcaaaaact gctaacgcct cggccccggc
     5401 ttccttcgtc accggtaact ggtatccaaa ggcgaacgag caactcgaca aggtgtattc
     5461 cgacgcgaac atcgtggtct atcgagactc gaaggttacg gacgtgccag acgcgaacgc
     5521 gattgcctgg ttgacgcccg cgctgagcga agcttgggcc tacatcaaag cgaactatcc
     5581 cgtattcagc gggccccaat tgcaagtgat cctcggcaat cagaagggct ccgccagcgt
     5641 cacttacgca tacagctctc aaaacgcgta tcggaatgcc attttcagct tctccgacaa
     5701 atggagcctc gccgacagac acgcctggaa gttcgagtcg ctgatgcacg agatgaatca
     5761 catcgtggag agcaacaaca acgagatgca aaactctccg agctattcgg cttggggaga
     5821 cagccgttgg ggcgagatct tcatgtacga cctgttcagt catctcgcca gcgtgcccgc
     5881 gaccggcgtc gaagccactc acgcccagac gctactcgat agctggtggg aagctcgcga
     5941 tggagtgggc gcgcactggt tgcagggctg gtactacccg ctttatacgg gcgcgctcgg
     6001 caacaatgcc cccgcgaacc gcggagtagc cttcctcaac aagtacttca gcttactcgc
     6061 ccagtactat cccaagttta cgaacgaata cgcgcgcacg atgaacttgg gtgagtatat
     6121 ccacttctgc tcggccgcgg ccgggctcga tctcaccacg gtcgcccgta gcgtgttccg
     6181 cttcgatcgg cgcccggagg tagaagctca acttgcgcag gcccagctcg actttccaga
     6241 tgtctacgcg ctctacggct ccaatcgcgc acccggcttc accttggatc caatcccggt
     6301 cagcggggcc gcgggcagcc cgttgacagg ccagactttg gctgaaaggg cgggcgaccc
     6361 tgatctgggc gcaacactca cttattccaa acagagcggc ccgacttggc tcagtgtcgc
     6421 ggccgacggc tcgatcactg ggaccccgcc cactgccggg acctatgccg ccgaagtccg
     6481 cgtgaccgac aacggcggcc tgttcgatac agccaccttg accgtcgtcg tgagtggcgg
     6541 agcctgcacc cccgaaacag acgccgcgtt ttgctcgcgc ctatccgctg cctgcggagc
     6601 gctcacgggt actgacaact gcggggcggc tcgcagcgtc gcgtcctgcg gttcgtgtaa
     6661 ctcgccactc acgtgtggcg gggccggcgt cgcgaatgca tgcggaagca ctggcggcac
     6721 ggggccatgc agcggactgt gcacgaatcc gatcgtcatt ccgtccgcag gcttcggctc
     6781 gggagcccta ggcaccgccg ccacctgtca cgaaagcaag gccaacctca tcgggagcaa
     6841 ctgcagcaac ttcaccgcgc gaaccttccg gatcaatagc gcgctcgcga cttgcaacgg
     6901 gcagccgatc ccgctgcccg ccaagagaaa cggtggctac tgcttccagg cgagtgccgg
     6961 cgagcccgct tgggcgacct tctcgacgta ctgaattcga agagagcccc tccggaaaca
     7021 ttgactgaat cgcccgatcc gcccccgaca tccgtcactt ctccatcttg acccggcacg
     7081 aaactccgga gatagagcgc gccatgagta cagttcattt ggttggaggc gagaagggcg
     7141 gcgtcggcaa gtcggtggtc gctcgcgtgc tggcacagta cctcgtggat cgagatttga
     7201 aattcgtcgg catcgatgcc gatcgctcac atggcgcgct cctccgcacc tatgcggact
     7261 ttacccaacc tgccgatctc gaggtcttcg ccagcgccga tcagatcatg gatcgcgcct
     7321 tgggctccga ccgccgcgtg ctcgtcgatc taccggcgca gagcgcacgc gcgttgcgcc
     7381 ggtggatgga cgcgggcggc gtcgtgcaat tcgcgcgcga tatggacgtg aaactcgtgt
     7441 tctggcacgt gagcgacggc gggttcgatt ccgtgagcga gctcgaacgc gcacaagcca
     7501 ccttcgagca gtcggtaacc tacgtcgtgg tccggaattt cggacgctct tccgattttg
     7561 cgcagctcga cgaatcgaaa gccctgcgcg atgccgtggg gcgagggggt cgggtcatcg
     7621 acctacctgc actggacccc gccacgatgt acaaggtcga tcgctttggc tcttccttct
     7681 gggccgccgt tcacgcccaa gagggtgaat tcgtactctc gcccatggag cgacgtcgag
     7741 ccgagctgtg gctctctcga gcctttcatg cgctggacag cctggagaac gtcatctaac
     7801 gtcctttttt tgggaaagcc acggaatcac gagcgcgact gcgctcgtgt cccgtccctt
     7861 cggtcacgag ctcgaaacat ggcccggcgc ggtctccgcg cccgggccac tcgataacgc
     7921 cggtacgaac gttgctaacc cctgcagcac gttaggacgt gcgcacggtg gcgaatctca
     7981 aattctatat gacccccggc tcctgcagca ccgggatcca cttattactc gaggaactcg
     8041 agctcttctt cgaagctcac gtggtgaact tgctggccgg ggatcacctc aagccagcat
     8101 ttcgggcgct aaatccgcgg ggcagcgtgc ccgtcttggt gcgaaccgac ggcaccgcgt
     8161 tgacagactt catttccatc gcccgctggc tcgcaagcac gcaccccaat caccatttgt
     8221 tgcccgaatc ggaccgagag acgtcccgcc tgctgcaagt catggagtac gtagtgggag
     8281 tcattcatgg cgagggcttc acgcgagttt tcgtatccga tcggtacgcc caggactcgc
     8341 ttggaaagac gtccaccgaa agggaaggac gccagatcgt aagccgggga ttcgatcggg
     8401 tggagacgat gatcttcggg tcaccgtata tatccgaggc attctcgatc gcggatgcag
     8461 cgctattcta cgtcgaattc tgggcggatc gtgagggcat cccgttgcca cccaaatgtg
     8521 agcgacatta caaagccatg ctcacccgtc gtgccgtacg ccaggtattg ggcgaggaag
     8581 gctaccatag cgtcctgcgc agataccctg cctgaaaggt cgcgcggcgt cggtcacgtc
     8641 cgtggctcga tcctagagct ctccccgccg agctgttgtc tgagtttggt gacttccttg
     8701 tcgcgattgt cgtgtttcgg ccaaggccgg gcccgaactc agccacgaat gcgtcatcgt
     8761 caatggcgcg catcttggat cgcgacccct tcaggcaagg caatggctca cgtcatcttt
     8821 tacgagaagc cgggttgtgc tggaaacgcc cgtcagaaag ccc
//
LOCUS       DASTKU010000744         5820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1262439, whole genome shotgun sequence.
ACCESSION   DASTKU010000744 DASTKU010000000
VERSION     DASTKU010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262439"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5820)
                     /locus_tag="VFQ61_26125"
     CDS             complement(<1..>5820)
                     /locus_tag="VFQ61_26125"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9958013.1"
                     /translation="RAWSTARGSRVEGEPDCWIRPLEAHSRQLAEQQALVCEGESLTY
                     RELWQRAQRVARGLRQLGVDRERCVGVCFERSTSMIVAMLGIWLAGGAFVPLDPSYPT
                     ERLLHMARDCGLRVALVDASGGRALRNTTVRTAQIAAWASDEHFHEGGESEARIPPPL
                     PQQLAYVIYTSGSSGRPKGVAVSHAAILRHAWAIGAEYGLKPGETMLQFASINFDGSL
                     DQWLAPLLRGAKVVLRGPDIWSAEQFCEAVRRQGIGVVDLMPAYLMEVCRWAKTEAKQ
                     LPLRVCAVGGEALPREGLELVCSVVAPATVINGYGPTECVVTPLIWSASADGAGATTG
                     YAPIGSPIGDRSCYVLDARLNLALPGVIGELYIGGLTLARGYYGQPALTAERFIPDPF
                     SNDGGRLYRTGDLVRWSLDGNLEYTGRQDRQIKLRGFRIEQGEIEAALLAHPAVQGAA
                     VVLQERPPAKQLVAYVAGSTGAVSEAELRAFLTQRLPEYMIPARIGVLGELPKTPAGK
                     VDVRSLPELDRAIAPETPSSVSRQPATPVECVLVEVIESVLGISGIGVDDNYFELGGD
                     SIVSIQVVSRARARGLRISPRDVFLHQTVARLAAIAGKVTPVCTEAAELSGVEVPLTP
                     IQQAFFEQGVPVRHHFNQAVLLRATRTLDSAALESAFRAVTQHHSSFRLRFEPREGAS
                     PRQYYRPQGSEQAWIETIPVRDATELEVACERAQRSLDLSRGPLHRAIHFVLADGSER
                     LLLVIHHLVVDGVSWRILLEDLGQTYLAARAGKNPREALPPATSSFSDWSRYQQQQAL
                     RAASELPYWEALLRGAQTRPPGAKGQFKGTHDQEQTIVVGLSPEHTSQLLRGAPAAYR
                     TRGAELLLCALGRALASRTGQSRVWVEVESHGRAGASGLEPSGTGKAGVATSETEHDV
                     APVADTMDLSRSVGWYTSFYPVCLPVGARDVVAQTASDPRELNPIAELGNCIGRVKDA
                     LRSVPWNGANFGPLRYLTDGEVAQRMAVLPRPWVTFNYLGQFDGSFAQELPFVPAQER
                     AGARVDPSAELDNWLVVNGQVFDGRLEFGFSFADCAFDRASIEAIAGAFQAELERIVS
                     HCAAGASAISASDFPLARLTASELDELALDPENVEDIYPLSPMQEGLMFHTLLEPERG
                     TYVNQLEVELSGLDVPRFQLAWDEVVARHAALRTGFRSLSESSRVVQVVYRSVPTQVM
                     VHDIGSVVSPREHAAELARREHALGFDLAVAPLSRLVVARLNDRVCRLIWTQHHLVLD
                     GWSSARLMSEVLSVYAGRELPVRGHSYRAYIEWLSSRDRAAARAHFQSRLALLDGPTR
                     IPADAACIGSDGYGCTTSELGAERTREMRRVAERARITPSTLVQGLLSLLMARHTGKS
                     AVAIGLTVSGRPAELSGVEETVGLFINSVPVVAVCDSALPTVRWLRTLQSDNLELKNH
                     EWAPLYQIQSWAGRAGQSLFDTLLVFENYPVDRALRQQNFGVELGALEVREDTHYTLT
                     VSVREGASGMVISWQNDRAKLTDHSTRAFADQFGQLLDQVIACPEVALGNLQIGHVTP
                     GESLESPARAAPESSLNVIQRLATVCATWPAEEAIECEGARLSFRELWSRSNSVARAL
                     LERGIGRESRVGVALERGPRLIAALIGVMKAGAAFVPLDTSYPAERLLAMVHDSGAEY
                     VLVEPGTHISIPQWCSNLKYIEVGEAGAADCSVEILDEQLAYIIYTSGSSGRPKGVAV
                     SHGALARHCASIALEYGLEHGQSMLQFASANFDGSLDQWLAPLTRGARIVMRGAELWD
                     CERTFEVLAEERVDVADFMPSFLIELAHWCEERKAQLDLRICAVGGEALTREGLASVQ
                     RVLSHGVVINGYGPTEAVVTPLTWPAAQDTTPSTPYAPIGRPLPGRTAHVLDRDMNPL
                     PPAVMGELYLGGPTLARGYHGHAAWTAERFLPDPFGPPG"
BASE COUNT         1119 a         1954 c         1749 g          998 t
ORIGIN      
        1 agcctggtgg gccaaacggg tccgggagaa accgctccgc cgtccacgcc gcgtgtccgt
       61 gataaccacg ggcgagagtg ggtccaccta agtagagttc tcccatcacc gcgggcggga
      121 gcgggttcat gtcgcgatcc agcacgtgtg cggttcgccc cggaaggggg cgacctatgg
      181 gcgcgtatgg cgtcgatggg gtcgtgtcct gcgccgctgg ccaggtgagc ggcgtcacta
      241 ccgcttcagt gggcccatag ccattgatta caacgccatg actcaacaca cgctggacgg
      301 aagcgagccc ctctcgagtg agagcctcgc ctcccaccgc gcagatccgc aaatcgagct
      361 gagcttttcg ttcttcacac cagtgagcca gttcgatcag aaacgagggc atgaagtcag
      421 caacgtcgac ccgctcctcg gcgagcacct cgaatgtccg ctcgcagtcc caaagctccg
      481 ctccacgcat gacgatccga gccccccgcg tcaggggagc taaccattga tcgagtgatc
      541 catcgaagtt cgcggatgcg aactgcaaca tgctttgacc gtgctccaac ccgtattcca
      601 gggcaatgct tgcgcaatgt cgggcaagcg cgccgtggct caccgcgacg cccttgggac
      661 gccccgagga ccctgaggtg tagatgatgt aagccagttg ctcatcgagg atctctacgg
      721 agcagtccgc tgctcccgct tcgccgactt cgatgtactt cagattggag caccattgtg
      781 ggatcgagat gtgagtgccc ggttcgacca gaacgtattc cgctccagaa tcatgaacca
      841 tcgccaatag acgctccgcg ggatagctcg tgtccagcgg cacgaacgcc gctcccgcct
      901 tcatcacgcc gatcaacgcg gcgatcaacc ttggacctcg ctccaaggct actcccacgc
      961 gactctcgcg cccgatgcca cgctctaaca gggcacgagc gaccgagttc gaccgcgacc
     1021 atagctcacg aaagctgagg cgcgccccct cacactcgat ggcctcttcg gccggccacg
     1081 tcgcgcagac cgtcgcgagt ctctggatca catttagact cgactccggc gcggctcgcg
     1141 cgggagactc gagcgactcc cccggggtga catgcccaat ctgcaggttg cccaaagcga
     1201 cctcgggaca ggcaatcacc tgatccagca attgcccgaa ttgatcggcg aacgcgcgag
     1261 tcgagtggtc agtgagcttc gcgcgatcgt tctgccagga gatgaccatt ccggacgctc
     1321 cctcgcgcac gctgaccgtc agggtgtagt gggtgtcctc ccgcacctcc agcgccccga
     1381 gctccacgcc gaagttctgc tggcgtagag cgcggtcgac tgggtagttt tcgaagacca
     1441 ggagtgtgtc gaacaagctc tggccggctc gacccgccca gctctggatc tggtacagcg
     1501 gtgcccattc gtgattcttc aactcgagat tgtcactttg cagcgtgcgg agccagcgca
     1561 cggtcggcag cgcggagtcg cacaccgcta cgacgggcac gctgttgatg aagagcccca
     1621 ccgtttcctc cactccgctc agctcagccg gacggcccga tacggtcagg ccgatggcga
     1681 cggcagactt ccccgtatgg cgcgccatca acagcgagag cagaccttgg acgagcgtgc
     1741 tcggggtaat ccgcgcccgc tccgcgactc gccgcatctc ccgtgtgcgc tctgcgccga
     1801 gctcgctcgt cgtgcagccg taaccgtcac tgccgataca cgcggcgtcg gcgggaatcc
     1861 gtgttggacc gtccagcagc gccaatcggc tttgaaagtg cgcccgggcc gccgcgcgat
     1921 cgcggctcga taaccattcg atataggctc gatacgagtg gccgcggacg ggcagctcgc
     1981 gcccggcgta aacggaaagc acctcggaca tgagacgagc gctgctccaa ccatcgagca
     2041 ccaggtggtg ctgggtccaa atcagcctac acacacggtc gtttagccga gcgaccacta
     2101 gacgcgacag cggagcgacc gccaaatcga agccgagggc gtgctcgcgg cgcgcgagct
     2161 ccgcggcgtg ctctcgaggg ctcactacgc tcccaatgtc gtgcaccatg acctgcgtcg
     2221 gcacgctccg atacacgacc tgcaccacgc gtgaactctc cgacaaggac ctaaatcccg
     2281 tacgcaaggc cgcgtgccga gcaacgacct cgtcccacgc aagctggaat cgcggcacgt
     2341 ccagcccgct gagctcgact tcgagctgat tcacgtacgt gccgcgttcc ggctcgagca
     2401 gagtgtggaa catcagccct tcctgcatcg gagaaagcgg ataaatgtct tctacattct
     2461 ccggatcgag cgccaattca tccagctccg atgccgtaag ccgcgcgagc gggaaatccg
     2521 aagcagatat cgcgctcgcg ccggccgcgc agtgcgaaac gatgcgctcg agttcagctt
     2581 ggaaggcgcc agcgatcgcc tcgatcgagg ctcgatcgaa ggcgcagtcc gcgaatgaga
     2641 acccgaattc tagacgaccg tcgaagactt gaccattcac taccagccaa ttgtccagct
     2701 ccgccgacgg gtccactcgt gcgcccgcac gctcctgagc cgggacgaac ggcaattcct
     2761 gcgcaaacga gccgtcgaat tgccccaagt agttgaaggt gacccacggc cgcggcaaca
     2821 ccgccatacg ctgcgctacc tccccgtcgg ttagatatcg cagcgggcca aagttggctc
     2881 cgttccaagg aacggagcgc agggcatctt tgacccggcc gatgcaatta ccaagctccg
     2941 cgataggatt gagctctcgt ggatccgacg cggtctgggc cacgacgtct cgcgcgccaa
     3001 ccggcagaca tacaggatag aagctcgtat accaacctac gctccgactc agatccattg
     3061 tatcggcaac gggagctaca tcatgttccg tttcggaggt cgctactccg gccttacctg
     3121 ttccggaagg ttccaagcct gaggcgcccg cgcgcccatg gctttcgact tcgacccaca
     3181 cccggctttg ccccgtgcga ctggcgagag cccgccccag ggcacacaac aacaactcgg
     3241 ccccacgcgt gcggtaggcg gcgggagcgc ctctcagaag ctggctcgtg tgctcagggg
     3301 atagaccgac gacgatcgtc tgctcttgat cgtgcgttcc tttgaactgg cccttcgccc
     3361 cggggggccg ggtctgggcg ccacgtagta atgcctccca atagggcaat tccgatgctg
     3421 cgcggagcgc ctgctgctgc tggtaccgtg accaatccga gaaggagctg gttgcgggtg
     3481 gcagtgcttc acgtggattc ttcccggcgc gcgccgcgag gtaggtttga cccaagtcct
     3541 cgagcagaat gcgccacgag actccatcta cgacgaggtg atgaatgacc agaagcaagc
     3601 gctcagagcc gtcggcgagc acgaagtgga tggcgcggtg gagcggcccc cgcgagagat
     3661 ccaagctacg ttgagcccgt tcgcacgcga cctcgagctc ggtcgcgtcc cgcaccggga
     3721 tggtctcgat ccacgcttgc tcggagccct ggggtcggta gtactgtctc ggcgacgcgc
     3781 cctcacgcgg ctcgaacctc agtcgaaatg aggagtggtg ctgcgtgacc gcgcgaaacg
     3841 cactctcgag cgcagccgag tccagtgttc gagtggccct caacaacacc gcctggttga
     3901 agtggtgacg aacaggcacg ccttgttcga agaaagcctg ttggatggga gtgagcggga
     3961 cctcgacacc cgaaagctca gccgcctccg tgcagactgg tgtgactttg cccgcgattg
     4021 ccgccaatcg agccaccgtc tggtgcagaa acacatctcg cgggctgatt cgcagtccac
     4081 gcgcgcgcgc gcgactcacc acctgaatcg atacgatcga atcccctcca agttcgaaat
     4141 aattgtcgtc tactccaatg ccactgatcc cgagtactga ttcaatcact tccacgagca
     4201 cgcactcgac aggcgtggcg ggctggcgcg agacgctcga aggcgtctcg ggggcgatcg
     4261 cgcggtcgag ctcgggcaag ctgcgtacat ccaccttgcc cgcgggcgtc ttcggcaatt
     4321 ctccgagcac accaatgcgt gcggggatca tgtattcggg caagcgctgg gtcaagaatg
     4381 cgcggagctc ggcttccgaa acggctccag tcgagcccgc aacgtaagcg acgagttgct
     4441 tggctggagg ccgctcttgc agcacgacgg cggcaccctg caccgcgggg tgcgcgagca
     4501 acgcagcctc gatttcccct tgctcgattc ggaatccccg cagcttaatc tgtcgatcct
     4561 gacgccccgt gtactccaaa ttgccgtcca aagaccaacg cacgagatct ccggttcggt
     4621 aaagccgccc accgtcgttc gagaacggat caggaatgaa gcgctctgcc gtaagcgcag
     4681 gttggccgta gtagccacgc gccaaggtga gccccccgat atagagttca ccgatcacac
     4741 ccggaagggc caggttcaat cgtgcgtcca atacgtagca agaacgatct ccaatcggtg
     4801 aaccaatcgg ggcgtatccg gtagtcgctc ccgccccatc ggctgacgcc gaccagatga
     4861 gtggcgtcac cacgcactcg gtcgggccgt agccgttgat gacagtggcc ggggctacca
     4921 cgctgcagac cagctccagg ccttcccgcg gcaacgcctc tccaccgacc gcgcacacgc
     4981 gcaagggcag ctgcttggct tcggtctttg cccagcgaca gacctccatc aggtaggcgg
     5041 gcatgaggtc aactacgccg attccctgtc ttcgaaccgc ttcgcagaac tgctcggcag
     5101 accaaatgtc cggcccgcgg agcacgacct tggcgccccg cagcagcgga gcgagccact
     5161 gatccagtga accatcgaag ttgatggacg cgaactgcag catcgtctcg ccgggcttta
     5221 ggccgtactc ggcgccgatc gcccaggcgt ggcggaggat cgccgcgtgg gacacggcga
     5281 ctcccttcgg gcgcccagaa gagcccgaag tgtagatgac gtaggcgagc tgctgcggca
     5341 gtggcggcgg gatccgagct tcactctctc cgccttcgtg gaaatgctcg tcactcgccc
     5401 aagcagcgat ctgcgccgtt ctcaccgtag tgtttctcag tgcacgccct ccgctcgcgt
     5461 ccacgagcgc tacgcggagc ccgcaatcgc gcgccatgtg caggaggcgc tcggtcgggt
     5521 acgacggatc cagaggcacg aacgcgccac ccgcgagcca aattccaagc atcgccacga
     5581 tcatgctagt cgagcgctcg aagcagacgc ccacgcatcg ttcgcgatcg acgccgagct
     5641 ggcgcagccc tcgcgcaacg cgctgcgcgc gctgccagag ctcgcgataa gtgaggcttt
     5701 ccccttcgca cacgagcgcc tgttgctcgg cgagctgtcg cgaatgcgcc tcgagcggtc
     5761 ggatccagca gtcgggttcg ccctcgactc tcgagccgcg cgcagtactc caggcccgga
//
LOCUS       DASTKU010000745         6040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1038091, whole genome shotgun sequence.
ACCESSION   DASTKU010000745 DASTKU010000000
VERSION     DASTKU010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038091"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="VFQ61_26130"
     CDS             complement(<1..1471)
                     /locus_tag="VFQ61_26130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958014.1"
                     /translation="MNLRLNWAGPSVLVLISACAAPTQEPSQNTAAQSGEAQSEEIGQ
                     TTPTLLTADVARSTDPKQALAPRALLRHRSRKDPDAKRHATGRRELTPQESQAYKLTT
                     PKVVAVRPTQLLVERMREASALKSAEAKTLESNVVPFGNDIVTASPGEASAPVPLAAA
                     TALPRAVDNSTSPAFPEIRDQGSENSCVAFAVGYYQYTYALGKLLGWNNKNSINTTKV
                     SPKFLYNLANSGGDYGTGPGTVHDILRTQGALTWSEFPYSGDASNPTNYLAWPTTAAL
                     WKSALRYKSLGPASVGSPTEPGGIEQAKSLLANGQVLTFSTYIYSWEFEQVDNDPSTS
                     ADDGLVGQQIATWQNGSDGGHEATLVGYNDDLWVDINANGVVDSGEKGAFKIANSWGA
                     SWKNAGYAWVAYDALRAVSQVPNGPSSDLRMPLIGGIMALAGANTNYQPKLLAEFTVS
                     TASRGELRLALQQTELDRADPVDEYQPTMFQFGGGNYAFD"
     gene            1950..4121
                     /locus_tag="VFQ61_26135"
     CDS             1950..4121
                     /locus_tag="VFQ61_26135"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HET9958015.1"
                     /translation="MTALGIRPFLVLDAPFRTRPCLSTLLLLGALGSACSGESQPGST
                     PSSGANAGWGNPSSSGGTSGGSGGSRPTGSAGAQGGSITSQGGRSSASGGRSTGNAGG
                     GTIPSGTPGELNYAEAFQKALMFYEFQRSGKLPADTRNNWRGDSGLKDGADAGLDLTG
                     GWYDAGDHVKFNLPMAYSAAMLAWSLLESPVAYSATGALDSSLANLKWATDYLIKCHP
                     EPNVYYYQVGDGNLDHAWWGPAEAMQMERPSFKVTASAGGSTVVAETAAALAAAALVF
                     QNSDPAYADQCLSHAKQLYEFAETTQSDVGYTAAANFYTSSGFWDELSWAGAWLHRAT
                     GDATYLQKAEQYVAKWGREQQTDVISYKWAHCWDDVHYGAELLLAQDTAKPTYVESIE
                     RHLDYWTSGVSGNRITVTPGGLAWLSQWGALRYATTTAFLATVWADSGHSSSAKRDVY
                     RTFAKTQADYALGSTGRSFEIGYGVNPPEHPHHRTAHSSWADSQTVPEKHRHTLVGAL
                     VGGPDSKDVYEDKIDNFINNEVACDYNAGFVGLLGKLTEKHGGTPITDLSAFETPSAD
                     EYGVDAAVNAQGPNFIEIKALVLNKSGWPARVDDQLSLRYFLDLSEFVQNGASAADVV
                     ISSNYSQGAKVTGLVPVDAARNLYYVNIDFAGTPVYPGGQSAHKKEVQFRIAGPQSSA
                     FFDAQNDWSFKGLSPTPSTPTPATAIPVYRAGQKLAGEEPR"
     gene            4404..>6040
                     /locus_tag="VFQ61_26140"
     CDS             4404..>6040
                     /locus_tag="VFQ61_26140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958016.1"
                     /translation="MMRSICTGLCALGFAFSVPLLSGCGISNDHDEPSTSGTFRMPLT
                     ALAGEHLYRLSNVSLRVDPGSLGLYGGDNPNEQYLTATLASGSYTAELTSWTLQRAVG
                     DGRFEPVAANLTSSPTVRFELLNGTTTTVEFQFETDGQIVTLGSGQLRISARVDEKQP
                     TCTVLGDDCPEGSWCAPSELTGAAVACVPAGTRAVGESCASPLDCVHNSACYAFGASA
                     ATCAQLCQPTEFGKSCETGGICRPRGADYGVCEPDGSEIEDCPTPSGFLPITDRVDMA
                     YDSKRCKLYVSTRGGALLAHDLRARTTEPLDLSVGEPSGGPLWGLDISPNRELLVVAD
                     GTYSSTQNWVHLIDLSQGAARDVRFNLGYSETGTYSAVFLDDQRVLVTSQLQGSGWQP
                     MRLVDLASGSVALVGNVRQSTLLSAGVDRSVIAYIEPETWYFGVYQPSEGTIRSGYLG
                     SYPTDSAASKNGSQVAISGSSGVQVYDRSLTRLATLGAGRPGLGVAYSPVGHVLYASY
                     ANSSNGIEAIDTTNFETLYTVDSNANLGWSGNTGFGEG"
BASE COUNT         1146 a         1870 c         1844 g         1180 t
ORIGIN      
        1 cgtcgaacgc gtaattgcca ccaccgaact gaaacatcgt cggctggtac tcgtccaccg
       61 gatcggcacg gtcgagttcc gtttgttgca aggccagcct cagttcacct cgagacgcgg
      121 tgctgaccgt aaactccgcc agtagttttg gttgatagtt ggtgttggcg cccgctagcg
      181 ccatgatccc accgatcagc ggcattctca ggtcactgct ggggccgttc gggacctggc
      241 tcacggcgcg tagcgcgtcg tacgctaccc aggcgtaccc ggcgttcttc caactcgcac
      301 cccaggagtt cgcaattttg aaggcgccct tctcaccgct gtccaccacg ccgttcgcgt
      361 tgatgtcgac ccacagatcg tcattgtacc cgaccagagt tgcctcgtgg cccccgtcgg
      421 aaccgttctg ccaggtggcg atctgctgcc cgaccaaccc atcgtccgcg gacgtcgagg
      481 ggtcgttatc gacttgttcg aactcccacg aatagatata cgtcgagaag gtcagcacct
      541 gcccattcgc cagcaggctc ttggcctgct cgatgccacc gggttcggtc gggctcccga
      601 cagaggcagg tcccaaggac ttgtagcgga gcgcgctctt ccacagcgcc gcggtcgtcg
      661 gccaagcaag gtagttggtt gggttggagg cgtcacccga ataagggaac tcactccagg
      721 tcaatgcacc ttgcgttcgc aggatatcgt gtaccgtgcc cggcccggtt ccgtaatcgc
      781 ccccagagtt ggccagattg tacaggaatt tgggcgaaac cttggtcgta ttgatcgagt
      841 tcttgttgtt ccagcccagg agcttaccca aggcgtaggt gtactgataa tagccaactg
      901 caaaggcaac gcaggagttc tcacttccct gatcgcggat ctccggaaat gccggcgacg
      961 tgctgttgtc caccgctcga ggtagagcgg tagctgcggc cagcggaacg ggcgcgctcg
     1021 cctccccggg gctcgcggta acgatatcgt taccgaacgg cacgacgttg gactccagag
     1081 tcttcgcttc ggcggacttc agtgccgacg cttcccgcat tcgctcgacc agaagctggg
     1141 ttggacggac tgcaaccacc tttggtgtgg tcagcttgta agcctgtgat tcttgggggg
     1201 tcagctcccg acgtccagtc gcgtgccgct tagcgtcggg atccttccgc gagcgatgcc
     1261 gaagcagcgc tcgtggggcg agcgcctgct tgggatccgt ggagcgcgcg acgtcggcag
     1321 tgagcagcgt cggtgtcgtc tgcccgatct cttcactctg tgcttcgccg ctttgtgcag
     1381 cagtgttctg agagggctcc tgagtgggag ccgcgcaagc agaaatgagt actagaacgc
     1441 ttggccccgc ccaattcaaa cgtagattca tcggtttcgt ctcctggccc gcattcgagc
     1501 gcggtctggt gtttcgacaa cggcactcga cacgtccggc cgtgactcat ccgggtgtga
     1561 gttgtgtcga cgcccactct ccccgctcga agaggggaat tatcaatcgc tggagttaga
     1621 ggcagcctcc ccctaaacct tcgcgaaatt atctcgtgtt tgtgtgacga gatgctcgcg
     1681 cgctaacccg gctacccgac ctgcaattac gatactcgca gacgttattg cgatgtgtct
     1741 cccgcagcgc tcgtggatgg gaggcctacc ggtctgctcg acgagccacc cgggcgctac
     1801 gatttggatg cccgccgtga cagggcttgc acgcggagca accttgcacc ccgactaata
     1861 gagagcagcc gcgtgtgagt caaaacagca gaaatgactt atgccggcgc tggctgtcgc
     1921 gccggactcc cccgagctaa gccaaaccgg tgactgccct tggaatccgc cccttccttg
     1981 tcctagatgc tccgttccgg acgcgcccct gcctaagcac gctgctcctg ctgggagcgc
     2041 tagggtcggc ttgttcagga gagagtcagc caggctcaac gccttcttct ggcgcgaatg
     2101 ccggctgggg gaacccgtct tcgagtggtg gtacaagcgg tggctcgggt ggctcgcgcc
     2161 ccaccggttc agcgggtgct caaggcggct cgatcacctc ccaaggcggg cgcagctccg
     2221 cctccggcgg ccggagcacg ggcaatgccg gcgggggcac cattcccagc ggcactccgg
     2281 gtgagctgaa ctacgccgag gcctttcaaa aggcgctcat gttctacgag tttcagcgat
     2341 ccggaaagct gcccgcggac acccgcaaca actggcgcgg ggactcgggc ctgaaagacg
     2401 gcgcggacgc aggtttggat ttgacgggag gctggtacga cgcaggagac cacgtcaagt
     2461 tcaacctacc catggcctac tcagcggcca tgttggcctg gagcttactc gagtcaccag
     2521 tggcttacag cgccactggc gcactggact cgagtctcgc gaacctgaag tgggccacgg
     2581 actacttgat caagtgccac ccagaaccga acgtctacta ctatcaggtg ggcgacggaa
     2641 acttggatca cgcctggtgg gggcccgccg aagcgatgca aatggagcgg ccttctttca
     2701 aggtcaccgc ctccgcgggc ggctcgacgg tggtcgccga aaccgcggcc gcgctggccg
     2761 ccgcggctct ggtgttccaa aacagcgatc ccgcgtatgc agaccagtgt ctgtcgcatg
     2821 ccaagcagct gtatgaattt gcggagacca cgcaaagcga cgtgggctac accgcagccg
     2881 cgaacttcta cacatcgagc ggcttttggg acgagctctc ttgggcgggc gcgtggctgc
     2941 accgcgcgac cggtgacgct acgtatctac agaaggcaga gcagtacgtt gcgaagtggg
     3001 gacgagaaca gcagacggat gtgatttcct acaagtgggc gcattgttgg gatgacgtgc
     3061 attacggcgc agagctgctg ctagcccaag acaccgccaa gcccacttac gtggagtcca
     3121 tcgagcgcca cctcgactat tggaccagcg gagtcagcgg caatcgcatc acggtcacgc
     3181 ccggcgggct cgcctggctc tcgcagtggg gagcattgcg ctacgccacc acgaccgcgt
     3241 ttctggcgac ggtatgggcg gactccgggc actctagttc cgcgaaacgg gacgtgtatc
     3301 gcaccttcgc caagactcag gctgattacg cactcggctc tacggggcgg agtttcgaaa
     3361 ttggatatgg cgtcaatcct cccgagcacc cccaccatcg gacggcgcat agttcgtggg
     3421 ccgacagcca gaccgtgccc gagaaacacc ggcacaccct ggtgggagca ttagtgggag
     3481 ggcccgacag caaagacgtt tacgaagaca aaatcgacaa cttcatcaac aacgaagtcg
     3541 cgtgcgatta caacgctgga ttcgtggggc tcctgggtaa gctcaccgag aagcacggcg
     3601 gaaccccgat cacggatctg agcgccttcg aaacccctag cgcggacgag tatggcgtag
     3661 atgccgcggt caacgcgcag ggcccgaact tcatcgaaat caaggctctg gtcttgaaca
     3721 agagcggttg gcccgcgcgc gtggatgacc agctctcatt gcgttacttt ctcgatttat
     3781 cggagttcgt ccagaacggc gcttccgccg ctgacgtcgt gatcagcagc aactacagcc
     3841 agggggccaa agtgacgggc ttggtcccag tcgatgccgc gcggaacctg tactacgtca
     3901 acatcgattt tgccggaaca ccggtttacc caggtgggca atccgcacac aagaaggaag
     3961 tgcagtttcg catcgcggga ccacagagct cggcgttctt cgacgctcaa aacgactggt
     4021 ctttcaaagg tctctcgccc accccgagca cgcccacccc ggcgacggcc atcccggtct
     4081 atcgcgccgg gcagaagctc gcgggcgaag agcctcgcta gaaacgccgt cggattacgt
     4141 tctcgttcaa ccaaaggcac ggtcaagggc tgggactcgg ataggtcggt ccactcgcca
     4201 tcgtttgcgg cgcggccaat cgccggtccc cttgagagac tcgtggtccg aaagaatatg
     4261 cgagggaggt cagaacttgg aagtttctga attctggtaa ccgccgcgac cgaaggcttc
     4321 atgtacaagt gcggccccgg cctggtattc aagccagagc cggagggggc tctgcccagc
     4381 gtgccgcgaa agggagtctg cgcatgatgc gttcgatttg cactggcctg tgcgccctcg
     4441 gtttcgcctt ctccgtcccg ctcctctcgg gatgcgggat ttcgaacgac cacgacgagc
     4501 cgagtacctc gggtacgttc cggatgccgc tgacggcctt ggccggcgag catttgtatc
     4561 gattgagcaa cgttagtctc cgggtcgacc cgggctcgct gggactgtac ggcggggaca
     4621 accccaacga acagtatttg accgcaacgc tcgcctcggg gtcttatacg gctgaactca
     4681 cgagttggac tttgcaacgt gcagtgggtg acggccgctt cgaaccagtc gcagccaatc
     4741 taacttcatc cccgacggtc cgtttcgaac tgttgaacgg gacgaccacc accgtcgaat
     4801 tccaattcga gactgacggg cagatcgtca cgctgggtag cggccaattg cgtatttccg
     4861 cccgggtgga cgagaaacag ccgacctgca ccgtcctcgg agacgattgt cctgaaggga
     4921 gttggtgtgc accctcggag cttaccggtg ccgccgtggc ctgcgttccg gcggggactc
     4981 gagcggtggg cgaatcctgc gcttcaccac tcgattgtgt gcataactcc gcctgctacg
     5041 cgttcggcgc gagcgccgcc acttgcgccc agctctgcca accaacggag tttgggaaga
     5101 gctgcgaaac gggtggtatt tgccgtcccc gcggcgccga ctacggagtt tgcgagccgg
     5161 acgggagcga aatcgaagac tgccccaccc catccgggtt tttgccgatc acggaccgcg
     5221 tggacatggc ctatgacagc aagcgctgca agctctatgt ctccactcgc ggtggggcgt
     5281 tgctcgccca tgacttgcgg gcccgcacca ccgaacccct cgatctgtcg gttggcgaac
     5341 cttcgggtgg gcccctttgg ggcctggaca tttcgccgaa tcgtgagctg ctcgtagttg
     5401 cggacggcac gtattcgagt actcaaaatt gggttcacct gatcgatctg agccaaggcg
     5461 ccgcaagaga cgtgcgcttc aacctaggat attcggaaac cgggacctac tctgccgtgt
     5521 tcctcgacga tcagcgggtg ctcgttacct cccaacttca gggctctggg tggcagccca
     5581 tgcgtctggt ggatctcgcg agcggctcgg tcgctttggt cggcaatgtg cgacaaagca
     5641 cgctgctgag cgccggcgtg gaccgctccg tgatcgcata catcgagccc gaaacatggt
     5701 acttcggagt ctatcaacca tcagaaggta cgattcgcag cggctatctc ggctcctacc
     5761 cgacggacag tgcggccagc aaaaatggct cgcaagttgc aatctcgggg tcttcgggag
     5821 tacaggttta cgatcgcagc ttgacccggc tcgccaccct gggcgcaggc cggcccggct
     5881 tgggagtcgc ctactcgccc gtcggccacg tactctatgc aagctacgcg aacagctcga
     5941 atggcatcga ggccatcgat accaccaact tcgagactct ctataccgtg gattccaacg
     6001 ccaatctagg ctggagtgga aacactggat ttggtgaggg
//
LOCUS       DASTKU010000746        13951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1933285, whole genome shotgun sequence.
ACCESSION   DASTKU010000746 DASTKU010000000
VERSION     DASTKU010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13951
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933285"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFQ61_26145"
     CDS             <1..825
                     /locus_tag="VFQ61_26145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958017.1"
                     /translation="QKRALKQAFGEVRVSNAIAVRAGLLKLPMSLYELLPIAAYEFGS
                     SSPVDSLIQDLGVGGRDIGAVVDIAPLHKKRYLHVELGAYNGDANGASNKPGLGLVGG
                     RITSRPISRLRLGFDCLYRPRAASDVNDTGTVYEKYSTGATCSADAEFSYHKFGIRGE
                     FLNGKRTDSPIFTDKSKHTFMAAWGIATYRIKLRGKSALIPAVRVEWLDEDRQSNVGG
                     SLLMSAAMNIDFTRNVRLLFDLSRHNVQVGTRARSAILPIYKPSATSGVIQLQLKL"
     gene            complement(873..2303)
                     /locus_tag="VFQ61_26150"
     CDS             complement(873..2303)
                     /locus_tag="VFQ61_26150"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9958018.1"
                     /translation="MKPNILIVDDDRSFRILTETALASEGFDVRVCPTLRRAREELYS
                     AAPDVLILDRRLPDGDGIDLLREISSTGTVATAVIVVTAYGDVDSAVSAMQAGAVDYL
                     TKPTSIADLLIKIQKVLETRGLKERLALADVANRKPPEVEPQSPALRRVHAELERLSR
                     SPQTPALLLGPSGAGKQYAAELLHRLTHPEQNAPFVDVNCAALPEQLVESELFGHERG
                     AFTDAKATRRGLIEMASGGTLFLDEVTELPEASQAKLLKFLDSGRFRRLGGQREIEVE
                     LRILAATNRDIHELVAAGRFREDLYHRLSVFIVRVPALSERREDIPLLAKSFVEFFGR
                     RVKKRIAGISDAALAALCRYDFPGNVRELRNIIERAVILSAGPILEESSLVIESRRIS
                     AARPAGFFNVDVLPDGSVPSLESVERAYVARVLEQMNGRRMLTAQVLGVSYPTFLKRL
                     REFGMSGDDLHRSDSDLSQIAVNGKA"
     gene            complement(2386..5556)
                     /locus_tag="VFQ61_26155"
     CDS             complement(2386..5556)
                     /locus_tag="VFQ61_26155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HET9958019.1"
                     /translation="MLERLLKFALDNRLAPFVLALVLAGWGLYAYRNLTIEAFPDPTD
                     TQVQLITLFPGQPTEEVERRVSLPLERALNGTPGLSRLRSISLFGLSFVTLTFEDGVD
                     PLIARQQTLERTASAELPEGVQPSLGPMATPIGEIYRYTLEGKNADPMTLRTLQEWVV
                     RPRLLQAAGVADVVTYGGLVQEVHVQPDPARMADLSIGLQDIFEALHKASSNATGGYI
                     ERGSEMFVIRSLGIFKNVPDLERVRVGFHDGVPVTIRDVASVSVGYAPRQGIVTREQN
                     EDAVEGIVLMRRGQNPSVVLENLKARIADLNQRALPDGIRLKAFYDRTELVNTTIKTV
                     FRNLAEGAVLVILVLLAFMLSLRAALIVAITIPLSLAASFAYLHTRGMSANLLSMGAV
                     DFGIIVDGAVILVEHLFHKLSPSHPNLGHANPGALDFGDEGVVEAAQPLKARLFHAAR
                     EVAKPTLFSLLIIIAAYLPIFSLQRVEGRIFSPLANTVVSALLGALLMTFTLVPVLCL
                     IALRKAKPTRDSPLLTLAQRVFDPSLRFAMNNVSAVLLLATFSLVSAFALLPRLGSEF
                     LPALNEGALYVTFTLPDNLSLTEGRKLAPRLREILHRTPEAVELLSQLGRPEDGTDPT
                     LPSNLEIFVKLKPLEQWRKNIHSLDDIIQEMAKNLQEVPGIEYNFSQPIRDNVAENIS
                     GQFGQIALKIYGDDLKELQEAADKATAVLNTVPGAADVGVVKSGETPQIAVRLDRNAL
                     SRFNLDLGDVQDYIETAMGGHVASELWDGERRFDVTVRLPPATREDANSIRRLMLPMH
                     MSADQRGQLIPLSAVAQVSMATGRAAITRENGRRYVGVRMNVRNRDLGSFVEEARQKF
                     AEKVRLPAGYEVSWGGEFENQQRAMQRLRLVIPLALLITFLLLFSAFGSLFDAVLILL
                     NVPFALIGGVIGLAWAGMPLSVSAAVGFIALLGQAVLNGVLVVASIRSHVMAGEPLRE
                     AAINGTRERLRAVLVTAFLASLGLLPAAMSHAIGSETQRPIAVVVVGGTISAALLTLI
                     VLPVTFYVGSLLVEAFRRPKLVEAGA"
     gene            complement(5568..6812)
                     /locus_tag="VFQ61_26160"
     CDS             complement(5568..6812)
                     /locus_tag="VFQ61_26160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9958020.1"
                     /translation="MNTEDLRSQTLEPSASLGTSFGPSRPVKLGDVLAVGRQRMWPFV
                     AALVIGAGALVVTLSFQRKPPEEMSDAPGIAVGKSDVTLAKDAPQWKLLHVTPAVAKG
                     ENFGEPFPARFGVDEALAAKVGTPLAGRVSTVHVVLGQNVKAGQPLFSVTSPDIAGLR
                     AEREKSAASLELAKAAYERVKAMVDARALPAKDALESNEQLREASLSLRLAESKLMSL
                     KVSSRADNEFTVVSPRDGVVIEKNVLPAQQVSTETPLISVADLSTVRVVAELFEADAA
                     GIGKGTQAIITSPSLPGLTLKASVELVSSVADPERHTVSVYARIPNPTGDIRPNAFAE
                     MQLSGSAPAGAVEIPTSALVSDGSHQYVYVQDRPGHFVRRPVVAGSSHSDKLVIYSGL
                     AVGELVVSAGAVLLDNQIALKD"
     gene            complement(6816..8204)
                     /locus_tag="VFQ61_26165"
     CDS             complement(6816..8204)
                     /locus_tag="VFQ61_26165"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9958021.1"
                     /translation="MRSSFINKSTGLLVVLVCLGWVRVAGAQTAPPPGANESAVAAPG
                     TANLGNSNGGASSLPRATVHQLLLDPAVLRERLASQSYDVLASKDRLAQAEASVGSAR
                     LVPNPSLDAALTGVPVSQSHDARFRTSSAWSVGLSQTVELGKRGPRTEAATLRQQAAQ
                     QELRATLGERLGVARLALANTLHLALRLKTLRESLADAERASGLERVRYEQKALSGMD
                     YDRLLLDLDALRSDVLHSEAEFQAALAECSARLLARCDLTESAEQDLVSALPLVLQNG
                     EANLRTRPDVMQFGFEQRAAVREAEYARRRALPDLTFRVGYTRDNAAGTGEALDSISV
                     GLSLPLPISDHGQYDARRALARASEMDHQRSAVLADARAELTGLLLRKAALENTVGVL
                     ERDSIPRAEGVLNSTQQAFDHGGISLTDLLLARRTYVALRLRLLEERFELFTLRNDLY
                     RVLGIDTRGAEK"
     gene            8600..9799
                     /locus_tag="VFQ61_26170"
     CDS             8600..9799
                     /locus_tag="VFQ61_26170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HET9958022.1"
                     /translation="MSVAPGRRMTGDSDAARSEATPLDNTRPDVGPAEATARHKFDER
                     PFIAIWEMTQACDLACKHCRASARPGRDTQELSTFEGKQLLSRLAEMRVPFVVLTGGD
                     PAKRPDLLELVQHGVEAGLHLGLTPSATPLVTDPLIRELAGRKLSRLAVSIDGPDAVV
                     HDTFRGVLGSFDEALRILNTARNSGITTQVNTTVHAGNIDQLARTADLVQGLGATLWS
                     VFFVVPTGRAEAKMLPDPEHVERILTELAELAETRSFALKTTAAPHYRRVLVQRRKRL
                     GSAPEHGVLGQRAMRINEGRGFVFISHRGQIFPSGFLPIECGNVRTRDLLDVYCNHPI
                     FQSLRDTNALHGKCGACDYRNICGGSRARSYALTGDYLESDVLCSYIPAGYEGPRPRI
                     HLDVLEG"
     gene            9805..11337
                     /gene="hemG"
                     /locus_tag="VFQ61_26175"
     CDS             9805..11337
                     /gene="hemG"
                     /locus_tag="VFQ61_26175"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HET9958023.1"
                     /translation="MTGAPEPPRVVVIGGGIAGVTAAYELRRRLPNVKLQLVEGRARL
                     GGNVRTEQHGPYLVDAGPDAFVRTKPAALELVRELGLERELIEPTPTPRGVYVAHRGR
                     LEPMPEGLMLGMPTRVAPLLQTPLLSLPGKLRMMAELTLPRGFGQHLRGPSSDESIAA
                     FMARRFGSEAAERLVTPLLSGIYAGLPDQLSAQATFPQLCELEERFGSITFGLLQLRA
                     RSAPGSDTTSSPRARRLSRFQAVHRFLRDSSPGSAESPFLSLRMGLGSLIDALAKTLE
                     PGSLRLGESVEELGYDAPSREWVVRTSRATWRADAVICAAPAHVAARIVPRASLAREL
                     SAIGYESTATVFFGMAADSVPRPLDASGFIVPPGEGGVLAATWVSSKWSDRAAPGQVL
                     VRAFLGGARRELNLDHSSDEYLIEIARYELERLMGPFSRVNYERVFRHTAANPQPGLL
                     HRTRLAQIRDELRYLPGLSLIGAGYDGVSLPDCIRQARTAVAALSHQLSVTSRAAPAT
                     AA"
     gene            11490..12737
                     /locus_tag="VFQ61_26180"
     CDS             11490..12737
                     /locus_tag="VFQ61_26180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor A family protein"
                     /protein_id="HET9958024.1"
                     /translation="MLACRLVACKLGIWALLACGCGAKGSESIPHAGAGGGSGSSHSS
                     APVSSGAAASTTAPSTSPSGTADHSLPPPSTTALIPSGTLPSEGTATETQVPDPSGTA
                     VPGETHDPPPGVTTDPPPGQTGDPPGGNTGDPPGGHTGDPPGGNTGDPPRDCVPSELD
                     AVNVIVFQDATPDGADVEGRMYVGHDATLEGYSIASREPDLDCNQFALVVGNDLHASG
                     GSVHAGQVSYGGTLDISAFSAACGISQGSATNPVDFAALEAKLTGYSVAFRNYPVNGV
                     ATLTDGTLLLAGTDPVLNVFSVSADQIASNVELSAPETASVIVNVSGTEITWQGKGFK
                     LPDGASCRGGSSAWCHRILFNFYEAEQINLAGIGIQGSILAPYATLDGGGGNVDGQVI
                     VRNLRGGIEYHPYFFTGCLKALP"
     gene            12755..>13951
                     /locus_tag="VFQ61_26185"
     CDS             12755..>13951
                     /locus_tag="VFQ61_26185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9958025.1"
                     /translation="MSRSMRCLLLLALLGASACSANSDPIRNEIGEGRATGGAGSGGS
                     SGGGKSGAVIPSSNTEGTGVPTDGTGGRTPDPGVQGCATSSKEAELTPVYQVFVYDKS
                     ASMGDDPNGQWQNRALRWDPLKQGMIDYFTNSGQTGVQASLEFFPAPGGKTQTCQANY
                     ASPSVPLTPLEKPATLIAALEAASPSGGTPTLSALYGAFKYAGELQAKNKAAKAMVVL
                     VTDGEPAIYNADTMQIETDCAPVGSTLTNTIPDIATLVAQAFQDTPSISTYVIGVGSG
                     DSLSSLATIAAAGGTELVLIDPVDPTRTRADLTERLRAIRSKHLSCDIAVPEPPPGQT
                     LDPSLINVDLTHGNGQHDELYRSDDCSRAGWRYDKPINPARIELCPTLCTSLESDPDA
                     KLDLVLG"
BASE COUNT         2566 a         4591 c         4381 g         2413 t
ORIGIN      
        1 caaaaacgcg cgctgaagca ggccttcggg gaagttcgag tgagcaacgc gatcgcggtg
       61 cgggccggct tactcaagct cccgatgtcg ctttacgagc tgctgccgat cgccgcctac
      121 gaattcggct cgtcatcgcc cgtcgactcg ttgattcaag acttgggcgt gggcggccga
      181 gacatcggcg cggtggtaga tatcgcaccg ctacacaaga agcgttactt gcacgtcgag
      241 ctcggcgcgt acaacggaga cgcgaacggc gccagcaaca agcccggcct cgggctcgtc
      301 ggcggacgca tcacgtctcg ccccatttcg cggctgcggc tgggcttcga ttgcttgtat
      361 cgcccgcggg ccgccagcga cgtgaacgac accggcacgg tgtacgagaa gtacagcacg
      421 ggcgccacct gtagtgctga tgccgagttc agctatcata agttcggaat tcgcggtgag
      481 ttcctgaacg gcaagcgcac ggacagcccg atcttcacgg acaaatcgaa acacacgttc
      541 atggcggcct gggggatcgc gacctaccga atcaagttga ggggtaagtc cgcgctgatc
      601 ccggccgtgc gggtggagtg gctggacgaa gatcgtcaaa gcaacgtggg cggcagcctg
      661 ctgatgagcg cggccatgaa catcgatttc acgcgcaacg tgcggctgtt gttcgatcta
      721 agtcgacaca acgtccaagt gggtacccgc gcgagatccg cgatcctgcc gatctacaag
      781 ccgagcgcca cgagcggcgt cattcagctc caattgaagc tttgagccgc tcgttggagc
      841 gcaccgctag aacaaggcgt ccggggcggc gctcaggctt ttccgttcac cgcgatttgg
      901 ctgaggtccg agtcggagcg atgcaagtcg tccccggaca tgccgaactc ccgcagtcgc
      961 ttcaagaacg tcggatagga gacgcccagc acttgggcgg tgagcatccg gcgcccattc
     1021 atctgttcga gcacacgcgc cacgtacgct cgctccaccg actcgagcga cggcacgctg
     1081 ccgtccggca acacgtcgac gttgaagaac cctgcgggcc tcgccgcgct gatccgacgt
     1141 gactcgatga ccagtgagct ttcctccaga atcgggcccg cgctgaggat caccgcgcgc
     1201 tcgatgatgt tgcgcagctc ccgcacgttg cctggaaaat cgtaacgaca caacgccgcg
     1261 agggcggcat ccgagatgcc ggcaatgcgc ttctttacgc gccggccgaa gaactcgacg
     1321 aaggacttcg cgagcagcgg gatgtcctca cgacgctcgc tgagcgccgg aactcggacg
     1381 atgaacaccg acagccgatg atagagatcc tcgcggaagc gccccgcggc gacgagctcg
     1441 tggatatcgc ggttggtagc ggccaaaatc cgcaattcaa cctcgatctc acgttggccg
     1501 cccaggcgtc ggaagcgccc gctgtccaag aacttgagca gcttggcctg cgaggcctcc
     1561 ggcagctcgg tgacctcgtc gagaaacaag gtgccgccgc tggccatctc gatcaatccg
     1621 cggcgcgtcg ctttggcgtc cgtgaaggcc ccccgctcgt gcccgaagag ctcgctctcc
     1681 accagctgtt cggggagcgc ggcgcagttt acgtcgacga acggggcgtt ctgttccggg
     1741 tgcgtcagcc gatgcaacag ctccgccgcg tactgcttac ccgcaccgga cgggccgagc
     1801 agcagagccg gcgtctgcgg gctacggctc agccgctcga gctccgcgtg aactcgacgc
     1861 aaggccgggc tctgtggctc gacttcaggc ggcttgcgat tggccacgtc cgccagcgcc
     1921 agccgctcct tcaggccgcg cgtctcgagc accttctgga tcttgatcag gaggtccgcg
     1981 atgctggtgg gtttggtcaa gtagtcgacc gcgcccgctt gcatggccga gacagcgctg
     2041 tccacgtcgc cgtaggcggt caccacaatc acggcggtcg ccaccgtgcc cgtgctgctg
     2101 atctcgcgca gcagatcgat cccgtctcca tccggaaggc gacgatccaa gatgagcacg
     2161 tccggagccg ccgagtacag ctcctcccga gcgcggcgga gagtcggaca gacgcggacg
     2221 tcgaagcctt cactggctag cgccgtctcg gtcaggattc ggaaacttcg gtcatcgtcg
     2281 acgatcagga tgttgggctt catgacgcct ctcgagcctc gacgagcgag gtactggtgt
     2341 tcgggtttcg atctcgatca caacggatgg cttcgagcga gccgctcacg cgcccgcctc
     2401 gacgagcttc ggccgcctga aggcctccac gaggaggctt ccgacataga aagtgacagg
     2461 caacacaatc aaggtgagta gagccgcgga gatggttcca cccacgacca cgaccgcgat
     2521 gggacgctgc gtctcactgc cgatggcgtg agacatagcg gcgggcagta gccccaagga
     2581 agctagaaat gcagtgacca gcacggcccg taggcgttcg cgcgtgccat tgatcgcagc
     2641 ttcccgcagc ggctcgcccg ccatcacgtg gcttcgaatc gacgctacca cgagcacccc
     2701 attgagcacg gcttggccga gcagcgcgat gaagcccacc gcggccgaga cggacagcgg
     2761 catccccgcc caggccaagc caatcacgcc accgatcagc gcaaatggca cgttcagcaa
     2821 gatgagcacc gcatcgaaca gcgatccgaa agccgagaac agcagcaaga acgtgatcag
     2881 cagcgcgagc gggatcacta gccgcagccg ctgcattgcg cgctgctggt tctcgaactc
     2941 acctccccaa ctcacttcgt agcccgctgg caggcgcacc ttctccgcga acttttggcg
     3001 cgcctcctcg acgaaggaac ccaagtcgcg gtttctgacg ttcatccgca caccgacgta
     3061 gcgacgaccg ttctcgcggg tgatcgcggc ccgccccgtg gccatgctga cctgcgccac
     3121 ggccgagagc ggtatgagct gcccgcgttg atctgccgac atgtgcatgg gcagcatcaa
     3181 ccggcggatg ctgttcgcgt cctcgcgcgt agcgggcggc aggcgtacgg tgacgtcgaa
     3241 gcgacgttcg ccgtcccaga gctcgctcgc gacgtgcccg cccatcgcgg tctcgatgta
     3301 gtcctgcaca tcgcccaaat cgaggttgaa gcgggacagc gcgttgcgat cgaggcgcac
     3361 ggcgatctga ggtgtctcgc ccgacttcac gaccccgaca tcggccgccc ccggcaccgt
     3421 gttcaacacc gcggtggcct tgtcggcggc ctcctgcagc tccttcagat cgtcgccata
     3481 gatcttgagc gcgatttgac cgaactggcc ggagatgttc tccgcgacgt tgtcacgaat
     3541 gggctgggaa aaattgtatt cgatgcccgg cacctcttgg aggttcttgg ccatctcctg
     3601 gatgatgtcg tcgagcgagt ggatgttctt gcgccattgc tcgagtggct tcagcttgac
     3661 gaagatctcg aggttggacg gcagagtcgg atcggtgcca tcctcgggcc ggcccagctg
     3721 ggacaacaac tccaccgcct cgggcgtgcg atgcaaaatc tcacgcagcc gcggcgctag
     3781 cttacgaccc tcggtcagag acagattgtc gggcagggtg aaggtcacgt acaacgcgcc
     3841 ttcgttcagc gccggcaaga actcggaacc caagcgcggc agcagagcga acgccgagac
     3901 gagagaaaac gtcgccagca gcagcacggc cgacacgttg ttcatcgcga agcgcaggct
     3961 cggatcgaag acgcgctgag cgagcgtgag caacggtgag tcacgagtgg gtttcgcctt
     4021 gcgcagcgca atcaggcaca gcaccggcac caacgtgaag gtcatcagca acgcgccgag
     4081 cagcgcgctc accacggtgt tggcgagcgg tgagaagatc cgtccctcca cccgttgcag
     4141 ggagaagatc gggagatacg cggcgatgat gatgagcagc gagaacagcg tgggtttggc
     4201 gacctcacgg gcggcgtgga acagtcgcgc cttcagcggc tgagcggctt cgaccactcc
     4261 ctcgtccccg aaatcgagcg cgcccgggtt ggcgtggccg aggttggggt ggctcggcga
     4321 gagcttgtgg aacaagtgct cgaccaagat caccgcgccg tcaacgatga tgccgaagtc
     4381 gacggcaccc atcgacagca gattggcgga catgccccgc gtatgcagat aggcaaacga
     4441 ggccgcgagg gagagcggga tagtgatcgc gacgatcagc gccgcccgca gcgacagcat
     4501 gaacgcgagc aacacgagaa tgaccaggac cgcgccctcg gcgaggttgc gaaacaccgt
     4561 cttgatcgtg gtgttgacga gctcggtccg gtcgtaaaag gccttcaatc gaatgccgtc
     4621 ggggagcgcc cgctggttga gatccgcaat ccgcgccttc aggttctcca gcaccacgct
     4681 cgggttctga ccacgtcgca tgagcacgat gccctctacg gcgtcttcgt tttgctcgcg
     4741 cgtgacgatg ccttggcgcg gagcgtaccc cacgctcacg ctcgccacgt cccggatggt
     4801 gaccggcacc ccatcgtgga agccaacccg cacccgctcc aagtccggca cgttcttgaa
     4861 gatgccgagg cttcgaatca cgaacatctc ggagccgcgc tcgatataac cgccggtcgc
     4921 gttggagctg gctttgtgca gcgcttcgaa gatgtcctgc aggccgatac tcaagtcggc
     4981 catgcgcgcc ggatccggct gcacgtggac ctcttgcacc aagccgccgt aggtcaccac
     5041 gtccgcgacg cccgccgctt gcagcagtcg cggtcgcacc acccactcct ggagggtgcg
     5101 gagcgtcatg ggatccgcgt tcttcccctc caaggtatag cgatagatct ccccaatcgg
     5161 agtcgccatt ggccccaagg aaggttgcac tccctccggc aactcggcgg aggcggtgcg
     5221 ctccaacgtc tgctgacgcg cgatcaacgg gtcgacgccg tcttcgaagg tcagagtcac
     5281 gaacgacagc ccaaacaacg agatactgcg taaacgggag agtccgggcg tgccgttcag
     5341 cgcccgttcg agcggcagag agacgcgccg ctctacctcc tccgtgggtt gccccgggaa
     5401 cagagtgatg agctggacct gagtatcggt cggatcgggg aacgcctcga tcgtcaggtt
     5461 gcgatacgca tagagccccc agccagcgag caccaaggcg agcacgaacg gtgcgagccg
     5521 gttgtccagt gcgaatttca gcagacgctc gagcatgtcg acctcggtca gtccttgagc
     5581 gcgatctgat tgtcgagcag cacggccccg gccgagacga ccagctcgcc cacggccagg
     5641 ccgctgtaaa tgaccagctt gtcgctgtgc gaggaccccg ccaccaccgg acgccgcacg
     5701 aagtggccag gccgatcttg tacgtacacg tattggtgcg aaccgtccga aacgagcgcg
     5761 ctggtgggaa tctcgaccgc gccagccggc gcggagcccg agagctgcat ctcggcgaat
     5821 gcattcgggc gaatgtcacc cgtaggattg ggaatacgcg cgtagaccga cacggtgtgt
     5881 cgctctggat ccgcgacgga agacaccaac tcgactgatg ccttcaaggt gagcccgggc
     5941 aaagacggac tggtgatgat ggcctgcgta cccttgccga taccggccgc gtcagcctcg
     6001 aagagctcgg ccaccacgcg gacggtagac aaatccgcga cgctgatcag cggagtctcg
     6061 gtgctgactt gctgagccgg tagtacgttc ttttcgatca cgacgccatc gcgcggggag
     6121 accacggtga actcgttatc agcccgagat gacaccttca gggacatcag cttcgactcg
     6181 gccaacctga gcgacaggct cgcttctcgc agctgctcgt tcgactccaa tgcgtctttc
     6241 gccggcaacg cccgcgcgtc caccatcgcc ttgacccgct cgtaggcggc cttggccagc
     6301 tcgagagaag cggccgactt ctcgcgctcg gcgcgtaagc cggcgatgtc ggggctcgtc
     6361 accgagaaga gcggttgccc cgccttgacg ttctgcccga gcacgacatg aacggtgctc
     6421 acccgacctg cgagcggcgt accaaccttc gccgccaacg cctcatcgac cccgaaacgc
     6481 gcggggaaag gctctccgaa gttctccccc ttcgcgacgg cgggcgtaac gtgcaggagc
     6541 ttccattgag gcgcatcctt ggccagcgtg acatcgctct tgcccacggc gattccggga
     6601 gcgtccgaca tttcttcggg tggcttacgc tgaaacgaga gcgtcacaac cagcgctccg
     6661 gctccgatta ccaaggccgc cacgaaaggc cacatgcgct gcctgcccac cgcgagcacg
     6721 tcgcccaact tcacgggccg cgaagggccg aacgaggtcc cgagcgaggc cgagggctct
     6781 agagtctgag accgcagatc ttcagtgttc atcgtttatt tctcggctcc tcgagtgtcg
     6841 atgccgagca ctcgatagag gtcgttacgg agagtgaaga gctcgaagcg ctcttccaac
     6901 agtctcaaac gcaaggcgac gtacgtgcgc cgcgcgagca ataggtccgt caagctaatt
     6961 cctccgtgat cgaaggcttg ctgcgtagag ttgagaaccc cctcggcgcg cgggatggag
     7021 tccctctcga gcacgccgac cgtgttctcc agcgctgcct tacgcagcaa gaggcccgta
     7081 agctcagcgc gcgcatcggc cagtaccgcg gagcgctgat gatccatttc gctggcgcgg
     7141 gccaacgcgc ggcgcgcatc gtactgccca tgatccgaga tcggcagcgg taggctgagc
     7201 ccgaccgaaa tactgtccag agcctctccg gtgccggcgg cgttgtcgcg cgtgtagccg
     7261 actcggaatg tcagatccgg caacgcccgg cggcgcgcgt actctgcctc acgcacggcg
     7321 gctcgttgct cgaagccgaa ctgcattacg tcggggcgcg tgcgcaggtt ggcctcgccg
     7381 ttctgcagca ctagcggcag cgcggacacc aagtcttgct ccgcgctctc ggtcagatcg
     7441 cagcgcgcca ggaggcgcgc cgaacactcc gcgagcgcag cctgaaactc ggcctcgctg
     7501 tgcaacacat cggagcgcaa cgcgtccaga tcgagcagca ggcgatcgta atccatgcca
     7561 ctcaaggctt tttgttcgta tcgaacccgc tcgaggccgc tcgcacgctc ggcatcggcc
     7621 aaagactcgc gtagagtctt gaggcgcagc gcgaggtgca gagtgttcgc gagcgccagg
     7681 cgcgcgaccc caagccgctc ccccaaagtg gcgcgcagct cttgttgtgc ggcctgttgg
     7741 cgcagagtgg ccgcctccgt gcgaggccct cgtttaccga gctcgaccgt ctggctcaga
     7801 ccgaccgacc acgcgctgct cgtgcgaaag cgtgcatcgt gactctgact caccggaacg
     7861 cccgtcagcg ccgcatccaa gcttggattc ggaaccaggc gcgcactccc tacgctggct
     7921 tccgcttggg cgaggcggtc cttggacgcg agtacgtcgt agctctggct cgccagacgc
     7981 tcgcgcaaca ccgccggatc gagcagcagc tgatgcacgg tggcgcgcgg caaactcgag
     8041 gcgccgccat tggagttacc gagattcgct gttcccgggg cagcaacggc ggactcgttg
     8101 gctcccggtg ggggcgcggt ctgagctcca gcgactcgga cccaacctag gcagacgagt
     8161 actaccagca ggccagtgct cttgttgatg aacgaacttc tcatgctcgg ctcagcgatt
     8221 acccgcagtc ggtccaggga ccggcgctgc tcctcgtgcg gcgaatgcac gcgaggcgac
     8281 cgctcactag gcacgaacca agccatgcca aaaaccgcgc tatttgcgtg gtttgtggca
     8341 gcgttcagcg gccgcactga aacccagttt cagcgcgcac tcaaggaact gaaacttcct
     8401 gccggcccgc tcgcctccta cccccggcgg ggatctcgac aagacgactc ctgtcggtga
     8461 ctcgccttca ggtgggttga cggagctcaa ctggactcgt cattcgaccg ctttaccgca
     8521 ggccttgcga tgcccatgcc ggcaccacgc agattctgca gccatattcc agctgaacta
     8581 acgcatttat gggctgggca tgagcgttgc accggggcgc cgcatgaccg gagactcgga
     8641 tgccgcgcga agcgaggcaa cgccgctcga caatactcgc cctgacgtcg gccccgctga
     8701 agcgacagcc aggcacaagt tcgacgagcg gcctttcatt gcgatttggg agatgactca
     8761 ggcgtgtgat cttgcgtgca agcactgtcg agcgtcggct cgccccgggc gagacactca
     8821 ggagctctcg accttcgagg gcaaacagct gttgagccgc ttggcggaga tgcgcgtccc
     8881 cttcgtcgtt ctgactggcg gtgatcccgc aaagcgcccc gatttgctcg agctggtcca
     8941 acatggcgtg gaagcaggtc tgcatctcgg cctcacgcct tcggcgacgc cgctcgtcac
     9001 cgatccgctg attcgcgagc ttgccggacg caaactgtcg cgcctagccg tcagcatcga
     9061 cgggcccgac gccgtggtac acgacacctt tcgcggtgtc ctggggagct tcgacgaggc
     9121 gctccgcatc ctgaacaccg cccgcaactc tggcatcacg acccaggtga acacaacggt
     9181 tcacgccggc aacatcgatc agctcgcgcg caccgcggac ttggttcagg gcctgggggc
     9241 cacgctttgg agcgtgttct tcgtggtccc aacggggcga gcggaggcca agatgctgcc
     9301 ggatccggaa catgtcgagc gcatcctgac cgagctcgcg gagctcgcag agacgcggag
     9361 cttcgcgctc aaaaccacgg cagccccgca ctaccgccgc gtgctggtcc agcgccgcaa
     9421 gcgtctcggc agcgcccccg agcacggtgt actcggacaa cgcgccatgc gcatcaacga
     9481 gggacgaggg ttcgtgttca tttcgcaccg cgggcagatc ttcccgagcg gcttcttgcc
     9541 gatcgagtgc ggcaacgtgc gcacccgcga tctgttggac gtgtactgca atcatcctat
     9601 ctttcagagc ctccgcgata ccaacgcgct gcacggcaag tgcggggcct gcgactaccg
     9661 gaacatctgc ggcggctcgc gcgcacgctc ttacgctctc accggggact acctggaatc
     9721 ggacgtgctg tgttcgtaca ttcccgcggg ttacgagggg ccgcgtccgc ggattcatct
     9781 cgacgtgctc gagggttgaa ggagatgacc ggcgccccgg agccaccgcg cgtggttgtg
     9841 attggcggcg gcattgcggg cgtgaccgcc gcgtacgagc tgcgacgccg cttgccgaat
     9901 gtgaagctgc agctcgtcga agggcgggcg cggctcggcg gtaacgtccg aaccgaacag
     9961 cacggtccat acctggtgga cgccggcccg gatgccttcg ttcggaccaa gcctgcggcg
    10021 ctggagctgg tgcgcgaact cgggctggag cgagagctaa tcgagccaac ccccacgccg
    10081 cgcggggtct acgttgccca tcgcggccgg ctcgaaccca tgcccgaggg tttgatgctg
    10141 ggcatgccga cgcgagtggc gccactactc cagacaccgc tcctcagtct gccaggcaag
    10201 ctgcggatga tggccgagct caccctcccg cgcggcttcg gtcaacacct gcgaggtcca
    10261 tcgagcgacg aatcgattgc cgcgttcatg gcccgccgct tcggcagcga agccgccgaa
    10321 cgcctggtca cccccttgct gtccgggatc tatgcgggtc tcccggatca gctcagcgcc
    10381 caagcgacct tcccgcaact ttgtgagctg gaggagcgct tcggtagtat caccttcggt
    10441 ttacttcagc tgcgggcgcg gtccgcgccg ggtagtgata cgacgagttc accccgagcc
    10501 aggcgtctct cgcgatttca agccgtacat cgttttctgc gtgactcctc cccaggttcg
    10561 gcggagagcc ccttcctctc cttacgtatg ggcctgggca gcctgatcga cgcactcgcc
    10621 aagaccctcg agcccggctc tttgaggctc ggagagtccg tcgaggagct cgggtatgac
    10681 gcgccatctc gcgagtgggt ggtgcgcaca tcgcgcgcaa catggcgcgc ggacgcggtg
    10741 atttgtgccg cgccggcgca cgtggcagct cggatcgtgc cgcgcgcgag cttggcgcgt
    10801 gagctctcgg ccatcggcta cgaatcgacc gccaccgtat ttttcggaat ggcagcggat
    10861 agcgtgccgc ggccgctcga tgcctctggc ttcatcgtac cgcctggcga gggtggagtg
    10921 ctggcagcga cttgggtctc gagcaagtgg agcgatcgcg ccgcgccggg ccaggtcttg
    10981 gtacgggcat tcttgggcgg agccaggcga gagctgaacc tggaccactc gagtgacgaa
    11041 tacctgatcg agatcgcccg ctacgagctc gagcgcttga tgggcccctt ctcacgagtg
    11101 aactacgagc gagtgtttcg ccacacggcc gccaatccgc aacctggctt gttgcaccgc
    11161 actcgtctcg cgcagattcg cgacgagctt cggtatttgc ctgggctgtc gttgattggc
    11221 gcgggctatg acggcgtgag tttgccagac tgcattcgcc aagcccgcac cgctgtcgcg
    11281 gccctctcgc atcagctgag cgtcacgtcg cgcgccgctc ctgccaccgc ggcctgagcg
    11341 ctttgatcac ggtccgggac gacccttgat cgcggtcaac tgctggggtg aagcgctagc
    11401 gtcgagcgaa ttcgaagcgt aagagtctaa ttggctgtcg gtgtgttcgc tggcccgttt
    11461 ggcgtgcccg acttgcgcgg agaagagcca tgctggcttg tcgactggtg gcttgtaaac
    11521 tcggtatctg ggccttactc gcttgcggct gtggcgccaa aggcagtgag tccatccccc
    11581 atgcgggtgc cggcgggggt agcggctcga gccattcctc ggcccccgtc tcctccggtg
    11641 ccgccgccag cactaccgct ccgagcacct cgccttcggg aaccgctgat cattcgctcc
    11701 cgcccccatc gacgaccgcg ctcatcccat caggcacctt accttcggaa ggcacagcta
    11761 ccgaaacgca agtgcccgat cccagtggca ccgcggtccc aggcgaaacg cacgatcctc
    11821 cgcccggcgt gacgacggat ccgcctccgg gtcagaccgg tgatccgccc ggaggaaata
    11881 cgggtgatcc acccggcggt catacgggtg acccgccggg cggaaacacc ggcgacccac
    11941 cccgtgactg cgtgccgagc gaactcgatg ccgtgaatgt gatcgtgttc caagatgcaa
    12001 cgccggatgg cgccgacgtg gaggggcgta tgtacgtcgg tcacgacgcc acgctcgagg
    12061 gttactcgat tgcgagccgc gagcccgatc tggactgcaa ccaattcgcc ctcgtggtcg
    12121 gcaatgattt gcatgcttcg ggtggctccg ttcatgccgg ccaagtgtcg tacgggggca
    12181 ccctcgatat ttcggcgttc tccgccgcgt gcggcatcag ccaaggatca gccacgaacc
    12241 ccgtagactt tgccgcgctc gaagcaaagc taacgggcta cagcgtagcg ttccgcaact
    12301 acccggtgaa tggggtcgcc acgctgactg acggcacgct tctgctcgcg ggcaccgatc
    12361 cggtgttgaa cgtgttcagc gtgagcgctg accaaatcgc gtccaatgta gagctgagcg
    12421 cgcctgaaac cgcctccgtg attgtcaacg tcagcggcac cgagatcacg tggcagggca
    12481 agggcttcaa acttccggat ggcgcatcgt gccggggggg cagctcggct tggtgtcacc
    12541 gcatcttgtt caacttctac gaggccgagc aaatcaactt ggcagggatt ggtattcagg
    12601 gctcgatcct tgcgccttac gccacgctcg acggcggcgg tggcaacgtg gacggccagg
    12661 tcatcgttcg caacttgcgg ggcgggattg aataccatcc ctacttcttc actggctgtc
    12721 tcaaggcact tccgtgatcg tgattggagt tcccgtgagc cgttccatgc gttgcctcct
    12781 actactcgcg ctgcttggcg ccagcgcttg ctcggccaac agtgatccca tacggaacga
    12841 aatcggagaa ggacgcgcca ccgggggagc aggcagcggc ggctccagcg gtggtggcaa
    12901 atcgggtgcg gtgattccga gttcgaacac ggaaggcacc ggcgtgccaa ccgatggaac
    12961 gggtggtcgt actccggacc cgggcgtaca aggctgcgcg acctccagta aggaggcgga
    13021 gctcacgcct gtttatcagg tcttcgtgta cgacaagtcg gccagcatgg gtgatgatcc
    13081 caatggccaa tggcagaacc gggccctgcg ctgggatccg ctcaaacaag gaatgatcga
    13141 ctacttcacg aattccgggc agaccggggt gcaagcttca ctcgagttct tccccgcgcc
    13201 gggagggaaa acccagactt gccaagccaa ctacgcttcg cccagtgtgc cactcactcc
    13261 cctcgaaaaa cccgcgaccc tgattgccgc cctggaagcg gcgagcccga gcgggggcac
    13321 gccgacgctc tcggcgctat acggcgcctt caagtatgcg ggtgagctcc aagccaagaa
    13381 caaagccgca aaagccatgg tcgtgctggt gacggacgga gagcccgcca tctacaacgc
    13441 agacacgatg cagatcgaga ccgactgcgc gcccgtgggc agcacgttga ccaacacgat
    13501 ccccgacatc gccacgctcg tagcacaggc tttccaggac accccgtcca tctcgactta
    13561 cgtcatcggc gtcgggagtg gggactcgct gagctcactc gcaaccatcg ccgccgcggg
    13621 gggcacggag ctcgtgttga tcgatcccgt cgatccgaca cgcactcgcg cagacttgac
    13681 cgagcgcttg cgggccatcc gcagcaagca cctgagttgc gacatcgcgg tgccggaacc
    13741 acctcccggt cagactctcg atccgagttt gatcaacgtg gacctcacgc acggcaacgg
    13801 ccaacacgac gagctctacc gctcggacga ctgcagccga gcgggctggc gctacgacaa
    13861 gcccatcaat cccgcgcgta tcgagctctg ccccaccctt tgcacctcgc tcgagagcga
    13921 tcccgacgcg aagctcgatc tggtgctggg c
//
LOCUS       DASTKU010000747         6321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_368200, whole genome shotgun sequence.
ACCESSION   DASTKU010000747 DASTKU010000000
VERSION     DASTKU010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368200"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..860
                     /locus_tag="VFQ61_26190"
     CDS             117..860
                     /locus_tag="VFQ61_26190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958026.1"
                     /translation="MTRQAPSTPTAADSGSPEPSQRAPVSGHAGSHAEAPSVSSSGFS
                     VSDYLQLAGMARRTVMLAFQESSGRLLGRIYIDAGEPRWAQDRFGLGEAAFRRLVTTD
                     RLDVVCNPVEAVPANYNLSGGLTELLMHAVHALDESRRSAGNVPPARAQQVDSAVRPI
                     PSHSVPVELVPVEIVRDLLRLFAEHVGPAAKPLLRQLLGELGCTAQTLPRAQLPEFVD
                     RMALAIPTLRARQGFVESVGEYWQRWSGE"
     gene            938..1765
                     /locus_tag="VFQ61_26195"
     CDS             938..1765
                     /locus_tag="VFQ61_26195"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HET9958027.1"
                     /translation="MWSTIQGRLLAILFGFLFLVGAGALASHVLLDRQTDDSLLVNLA
                     GRQRMLTQKMAKEATQLALLRREQGEGTRVTLEQRDQLVNSMRVFEMTLFALRDGGPA
                     PLNLEITRVRQTPSASSSDITRQLDRVVNVWKPFKAGLNTLIRTDGSDAAALHSVLET
                     NLTLLEQMNLAVNLMQQDSERRVYLLFWVQLSLLVASVALGIAGLWVARSSIARPLEE
                     LTQAAHHMSTGDLRVELPREGTREIQDLSASFERMRTSMLATLGGARYAQDPLDDLL"
     gene            2291..5836
                     /locus_tag="VFQ61_26200"
     CDS             2291..5836
                     /locus_tag="VFQ61_26200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HET9958028.1"
                     /translation="MPATDSDRAPSSSSAAPPSADRPRSARAFAGHPVDVATGEQFTA
                     AHDVEVPGVSPLIFRRVYNTAFLSRAPSVLGPGWVHAFEASLRRDLDGYVFEGHDGDR
                     VEFDDVDGSFSSGKNGASIVDRAACMELRREGEQLVVYHWHSVDEPVQKYVFNTRDGD
                     LMWLAARVLPSGHGLRIDRDRLGRALTITQTQERRRLYFDYDDQGRLSTLHLGFAAQP
                     RREARLVARYEYDARSRLMAVLDAQGAARCYEYDSEHRLISERGRRGGVFRMRYDAQG
                     RCVETSGDGDYRKCVFHYEPGRTTRVTDSLGFDTIYQYNAQGQVHHRILPNGAQHLTE
                     LDDYGRIVGEIDPLGGATRYVYDEIGRVAEKIFPSGAKIQYEYDEYHQPRRISEPDGA
                     VWEFRYERGALVEVKDPFGRRTSYHRGPYNDLRGAATSSGQELRIDTDEHFTEETLTD
                     QFGIITRRKLDLYLNAIESSDAEGPLGKAQYDALGRLIRSERPDGSARQFEYDAEGDL
                     TRMTDARGGVWQARYTPYGDCVEQLDPLGRQHRFTWDTEGRLTSIVNPKGETASFEYD
                     LAGNLVAVTHFDGSREQASYDLAARLTGRVRPDGTRLSFDRDEVGNLLKLSVVDREKG
                     EVPLRSFKYDSCGSPLEAKSSDAQVLFEYAVGGRLEAEVQNGRRIEYLYDARGYLSQR
                     SFEGSKAGPLRFEYDTRGRLRRFATQNGQGQTYDYDRRDQCIERTLGLPGGAFVPSLE
                     VAPLPPPQVETRKYDLEGRLRHQSVGKHGSRTYQYDAEGSLIDLADQLRGRRQYRYDL
                     ADQLITSVSRALGQHGYNYDANGNLTGKDHHTLGYASGNRLEALRGFGGDTTFARNVN
                     GELTRQTSAARDDQYTWDALGQLTSVLHRDGSVTEFAYDALGRRVHKEHRKPGQAAGN
                     GRTTRYLWSGDDLLAEQTRAPNPADLTVTPGESLTEYAMWGFVAEALWEDGKIRHVVS
                     SQQGVPQELIDEKGRLVWQGTYDDWGKLVAESGETTCRLRLPGQLADEETGLHYNRFR
                     YYAPETGQFVSPDPIGYGRSKNRFRFAPNAIAWIDVVGLECWAHAAQQDGAKRRAPSP
                     GDVHFPSREAALEEAARRHGIDPSTLEAQPQYGSNPNLVGPRGQPWERVSGLNANADI
                     VTFDHHSNGHYFRDAGEYEFPHYHGPSGEHLTYEP"
BASE COUNT         1222 a         1981 c         2020 g         1098 t
ORIGIN      
        1 tgcccgccag tgaatggtaa gtggcacgcg tcatgccact cggtagatga gcgacccctg
       61 cagcccagaa gcaccacttc cgcccagctt ctcgcgcggt tcgcaagtct tccaacatga
      121 cgcgccaagc cccctccacg cccaccgcgg ctgactccgg ttcacccgag cccagccagc
      181 gggctcccgt gtcgggccac gctggcagtc acgcagaggc tccttcggtc tcatcttccg
      241 ggttcagtgt ctcggactac ttgcagctcg ctggaatggc gcgccggacc gtgatgctcg
      301 cgtttcagga gtcctcaggg cgcttgttgg ggcgcatcta catcgacgcg ggcgagccgc
      361 gctgggctca agatcgcttt ggcctcggag aggccgcgtt ccggcgtctg gtcaccaccg
      421 atcggctgga cgtggtgtgc aatccagtcg aagccgtgcc cgcaaactat aacctgagtg
      481 gaggcctgac cgagctcctg atgcacgcgg ttcacgcgct ggacgagagt cggcggtcgg
      541 ctggcaatgt gccccccgca cgcgcgcaac aggtggactc tgcggtgcgg cccatcccaa
      601 gccacagcgt tccggtggag ctcgtgccgg tcgagatcgt gcgtgatctg ctcaggttgt
      661 tcgccgagca tgtcggtccg gcggcgaagc cgctgctgcg ccagctgctg ggcgagctgg
      721 gttgcaccgc gcagacgctg ccgcgcgctc agctccctga gttcgtcgac cggatggcgc
      781 tcgcgattcc caccctccgg gcgcgacagg gcttcgtgga gtcggtgggt gagtattggc
      841 aacgttggag tggagagtga gctccctggc ctcggcgagc cggcggcacg ccccaggatg
      901 ctgaagtcac tccgagcggc gctcgaggaa cgtatacatg tggtccacga ttcaaggcag
      961 acttctagcg attttgttcg ggttcctgtt cctcgtcggg gcgggggcgc tcgcgagtca
     1021 cgtcttgctc gatcgtcaga cggacgacag tttgctggtc aacctggcgg gtcgacagcg
     1081 catgttgacg cagaagatgg ccaaggaggc gacccagctc gcgctgctgc gacgtgagca
     1141 gggtgaagga acccgggtga ccctggagca gcgggatcaa ctcgtgaata gtatgcgcgt
     1201 cttcgagatg acgctgtttg ccctgcgcga cggcggcccc gccccgctca atctggagat
     1261 tacgcgggtt cgccagacgc caagcgcttc gagctcggat atcacacggc agttggaccg
     1321 ggtggtgaac gtctggaagc cgttcaaggc agggctcaac acgttgattc gcacggacgg
     1381 ctccgacgcg gcggctttgc atagtgtgct ggagacgaac ctcaccttgc tggagcagat
     1441 gaacctagcc gtcaatctga tgcagcagga ctcggagcgg cgtgtgtact tgctgttttg
     1501 ggtgcagctc tcgctgttgg tggccagcgt cgcgttgggg attgccgggc tgtgggtggc
     1561 gcggagcagc atcgcccgtc cgctcgagga gctgacgcag gcggcgcatc acatgagcac
     1621 gggcgatctc cgcgtggagc taccccgcga ggggacgcgc gagatccaag acctgagcgc
     1681 ttcgttcgag cggatgcgga cgagtatgct ggccactctg ggcggcgccc gctacgcgca
     1741 ggatcctctc gacgacctgc tttgaagtct aggttcagca actcgcggag caagccggtc
     1801 gcagctcccc ctgctggaag cactcgcggg tcggtctgat tacggccgcc ctacagggtg
     1861 ttcgtcgcgt gggttctacc gcgacccaag catcgagggc tcaaggatgg cagcggaagc
     1921 aacaataccg gttctgggcg acagccagaa cggggcccgc gaacacccca tcccagtcgc
     1981 gctctcagcg gcaccgattg atcgggcccg gcccagcaac gtgcgcgcca gcccaggtag
     2041 cccatcgtga gcgcagacgt tatccgatgg ggagcgtcgc gcccaccgcg ccggccccgc
     2101 ttcgtaccgt gcgccacctc gcgtcagccc cgcctcgtgc agagcgccgc gatgcgcgag
     2161 tgggacaagg cggcggagac gagacggaaa tcctgaatgg cgtgggcacg ccgacccgct
     2221 gaacacccta aggcttggga cgcactgcac gctgtacgac cccccctagt gcgatagaac
     2281 ggagatccca atgcccgcca ccgactccga tcgcgctcca agctcgtcga gcgcagcgcc
     2341 cccgagcgca gatcgcccca gatcagcgcg agccttcgcg ggccaccccg tggacgtagc
     2401 aacgggcgag caattcacgg ccgctcatga cgtggaggtg cccggcgttt cacccctgat
     2461 cttccgtcgg gtctacaata cagcctttct gtcccgcgcc ccgagcgtgc tcgggcctgg
     2521 ttgggtgcac gctttcgaag cgagcttaag acgggacctg gatgggtacg tattcgaggg
     2581 gcacgacggt gatcgggtcg agttcgacga tgtggatggc agcttctcga gcgggaagaa
     2641 cggtgcgtca atcgtcgatc gcgccgcctg tatggagctg cgacgtgagg gggagcagct
     2701 cgtcgtctat cactggcata gtgtcgatga gccggttcaa aagtacgtgt tcaatacccg
     2761 ggacggtgat ctcatgtggc tcgcggcgcg ggtgttgccg tcggggcacg ggctgcggat
     2821 cgatcgagac cgtttggggc gcgcgctcac gatcacgcaa acgcaggagc gccgccgact
     2881 gtacttcgac tatgacgacc agggtcgatt gagcactctc caccttgggt tcgcggcgca
     2941 acccaggcgg gaagcccgtt tggtggcccg ttacgaatac gatgcgcgct cacgcttgat
     3001 ggcagtattg gacgctcagg gcgcggcccg atgttacgaa tacgactccg agcatcgatt
     3061 gatctcggaa agaggccgcc gtggcggcgt ctttcgaatg cgctatgacg ctcaggggcg
     3121 ttgcgtcgag acgagcggcg acggggatta tcgcaagtgc gtctttcact atgagccagg
     3181 ccgtacgacg cgcgtgaccg atagcctcgg cttcgacacc atttatcagt acaacgcgca
     3241 gggccaagtc caccatcgga tcctgccaaa cggtgcgcag catctcaccg agctcgacga
     3301 ttacgggcgc atcgtcggcg agatcgatcc gctcggcgga gccacacgct acgtctatga
     3361 cgagataggc cgtgttgcag aaaagatatt cccgagcggc gccaagatcc aatacgaata
     3421 cgacgaatac caccaaccgc ggcgcatctc cgagccggac ggcgccgtct gggagtttcg
     3481 gtatgagcgc ggggcgcttg tggaggtcaa agatccgttt ggtcgccgaa cgagctacca
     3541 ccgcgggccg tacaacgacc tgcgcggagc ggcgacgagc agcggacagg aactgcgtat
     3601 cgacaccgac gaacacttca ccgaagagac gcttaccgat caattcggta tcatcactcg
     3661 ccgtaagctg gatctctatc tgaacgcgat cgagagcagc gacgccgaag gcccactcgg
     3721 caaggcccag tacgatgcgc tgggtcgcct gattcgcagc gagcgcccgg acggcagcgc
     3781 gcgccaattc gaatacgacg cggaaggcga cctcactcgt atgaccgatg cccggggtgg
     3841 ggtctggcag gcgcgatata cgccctacgg cgattgtgta gagcaactcg atccgctcgg
     3901 tcgccagcat cgattcacgt gggacaccga aggtcgactg acgtccatag tcaaccccaa
     3961 aggtgaaacg gcgagcttcg agtatgacct ggccggcaac ctggttgccg tcacgcactt
     4021 cgatggttca cgagagcaag ccagttacga tctcgcggcc cggctcaccg gtcgtgttcg
     4081 cccagacggc acccggctca gcttcgatcg ggacgaggtg ggcaacctcc tgaagctaag
     4141 cgtggtcgac cgcgaaaagg gtgaggtccc tcttcgcagc ttcaaatatg actcgtgtgg
     4201 gagtccactg gaagccaaga gctcggatgc gcaggtcctg ttcgaatacg cggtgggagg
     4261 gcgcctagaa gcagaagtcc agaacgggcg acgcatcgaa tacctgtacg acgcgcgcgg
     4321 ctacctgagt cagcgcagct tcgagggcag caaggccgga ccactccgct tcgagtacga
     4381 cacgcgaggc cgcctccgcc gcttcgccac ccaaaacggc caaggccaaa cctacgatta
     4441 cgaccggcga gaccagtgca tcgaacgcac cctggggctt cccggaggcg cattcgtccc
     4501 ctcgctcgaa gtcgcgcccc tgccgccgcc ccaagtcgaa actcgcaagt acgatctgga
     4561 aggcaggctc cgtcaccaat cagtgggcaa acatggcagc cgcacttatc aatatgacgc
     4621 cgaaggctcg ctcatcgatc tcgcggacca actgcgtggc cgccgccaat atcgttacga
     4681 ccttgccgat caactcatca ccagcgtaag ccgcgccctc ggccagcacg gctacaacta
     4741 cgacgccaac ggcaacctca ctggcaagga tcaccacacc ctcggctacg cgagcggcaa
     4801 tcgcctcgaa gcgcttcgag gcttcggcgg agacaccacc ttcgcccgca acgtcaacgg
     4861 cgaactcacg cgccagactt ctgccgcgcg cgacgaccaa tacacgtggg acgcgctcgg
     4921 acaactcacg agcgtcctgc accgggacgg cagcgtcacc gaattcgcgt acgacgccct
     4981 tggacgacgc gtacacaaag aacatcgcaa accggggcaa gcggcgggca acggaaggac
     5041 tacacgctac ctctggtccg gcgacgacct cctcgcggag caaactcgag cgcccaatcc
     5101 ggcggacctg acagtcaccc cgggcgagtc cctcaccgaa tacgccatgt ggggcttcgt
     5161 cgcggaggca ctctgggaag acggcaagat tcggcatgta gtctcgtcgc agcagggcgt
     5221 acctcaggag ctcatcgacg agaaaggtcg gctcgtctgg caaggcacct atgatgactg
     5281 gggcaaactc gttgcggaaa gcggggaaac gacctgtcgc ttgcggcttc ccgggcaact
     5341 cgccgacgaa gagaccggtc tgcactacaa ccgctttcgg tactacgcgc cggaaacagg
     5401 acagtttgtt tcgccggatc cgatcgggta cgggcgcagt aagaaccggt ttcggttcgc
     5461 acccaacgca atcgcgtgga ttgacgtggt cgggctcgag tgctgggcgc acgccgctca
     5521 acaagacggg gcgaaacgca gggctcccag ccctggagat gtccatttcc cgtcccgaga
     5581 ggccgcacta gaagaggcgg cccgaaggca cggcatcgat ccgagcacat tggaagctca
     5641 accgcagtac gggtccaacc ccaatctcgt tggtccgaga gggcagcctt gggagcgggt
     5701 cagtggattg aacgcgaacg ccgacatcgt caccttcgat catcattcga atggccacta
     5761 ctttcgagat gctggtgaat acgaattccc tcattaccac ggacctagtg gggagcactt
     5821 gacctatgag ccctagctat gacttctggt cgccaatatg cgcgctggct gcgaaagtcc
     5881 gatgctggac gaggttcgcg gttgaagaca acgagtttcc aacgatcggt ccatggggaa
     5941 tgctcctgac cattccagtg gatggatatc ttgaaacctc ggggggtccg gaaccgatgg
     6001 gtcgagttcg ttgggtacaa atggcgctcg agaggccggc aggtggtcgt gccggcatcc
     6061 ctcttcactt caaagatatc tcgggcgagt tgctgccttg gttcgcgagt caaacctttc
     6121 actgggagac aagacagacg tcgtggtccg ttgacgggat tttcgaagaa cgactcgtac
     6181 gaaccgtgca catacctaac ccgttctggc agccgcgcca ctgagggact gtgccggagc
     6241 tcccgggcgc ttggtctgcc ttcgccgtga gtgcgtttga gatgttgcgc atgtgcgagt
     6301 accgcgtgac agcgctgacg g
//
LOCUS       DASTKU010000748         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_592898, whole genome shotgun sequence.
ACCESSION   DASTKU010000748 DASTKU010000000
VERSION     DASTKU010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592898"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /gene="tmk"
                     /locus_tag="VFQ61_26205"
     CDS             complement(<1..979)
                     /gene="tmk"
                     /locus_tag="VFQ61_26205"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET9958029.1"
                     /translation="MFVVFEGIDGSGKTTVSNQVAERLAAGGLSVKHLRAEGKFVSSV
                     SEAIRDLGRDARNLELVPQAEFLLYVARDVQLIEQALRPALGKSDVVLADRFLFTAEN
                     MGENGRHLPRDFTQPILRAAAGGLRPDLVILVDVDPVLARARRKAYKLAVSDSRPPSR
                     KGLSGVGLQHRQRRGYLEMAAREPEQWVVVKNEDSLEETVSRVTELIRTAQRDGVPTE
                     LAGFRTLVEAAQSKPGAAPRTNEDAATAFFAWIDNRMQREPRVAAYMLGGMHGPGVDE
                     RRRDLAESVPEAVLAALTGLTDEPSWALRERLREKHARAVAKTLSGIPNE"
     gene            complement(979..2421)
                     /locus_tag="VFQ61_26210"
     CDS             complement(979..2421)
                     /locus_tag="VFQ61_26210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958030.1"
                     /translation="MPRLCSQGRVTLRRYGVGGVGLCVSSLCFAPGVAEAVEPGATSA
                     EGETANDDEPRSGDSRSASRPTDSAARTKAAAEAHQTPTEESASTGEEPHPADQAASE
                     ATTSDGTERPSGDKHPAKKHKKHRNSILHGGNTGHLEVRGRVFALSELTRRRRATPDL
                     SGFEPYNAWQLSLASARMALDYESPMPWLSAQVELELGDGISLKDGFIQARDEHFTVR
                     AGQFKMPISPLEVESPWSLPIVRRGFMHRLLNDWLDIGGRSPGILVGVRDKHGIRPSL
                     TAAVFQGRTLRRVPAGVNRDTTGIGAVSMDSQSYVLRAGVEILHIGLGAWYEYRVGSP
                     SLGVSDRFHTGGVDATLDLQLPHGGLRVWSELVAGESWYKHRYKTSTENPIFVEGRAL
                     VALRFGGVEDDDFYVEPFGMFGGFDADRDIRSDLAWEAAAGMNVGFWKRARLTLQGEV
                     NRTGENFPETFFPNSNVERESLLLQAGVAW"
     gene            complement(3023..>3257)
                     /locus_tag="VFQ61_26215"
     CDS             complement(3023..>3257)
                     /locus_tag="VFQ61_26215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958031.1"
                     /translation="GRVEEELEVPPSPLVGPAPDDVEPASVVPDPPFDEGFVGGRVVL
                     GVFSELPPELVLSTFETARAAVRFALPAESDAP"
BASE COUNT          621 a         1063 c          970 g          603 t
ORIGIN      
        1 cttcgttcgg gatgccggac agcgtcttcg ccaccgcgcg ggcgtgcttc tcgcgcaggc
       61 gctcacgcag cgcccagctc ggctcatccg tcaaacccgt gagcgcggcg agcacagctt
      121 cgggtacgct ttccgcgaga tcccgccggc gctcatccac gcccgggccg tgcatccccc
      181 cgagcatgta agccgccacc cgcggctctc gctgcatgcg gttgtcgatc caagcgaaga
      241 acgcggtggc cgcgtcctcg ttcgtgcgcg gcgccgcgcc gggtttgctc tgggccgcct
      301 cgaccaacgt gcgaaacccg gcaagctcag tgggcacgcc gtcccgctgc gcggtgcgga
      361 tcaattcggt aactctcgag accgtctcct ccagagagtc ctcgttcttg accaccaccc
      421 attgctcggg ctcgcgcgcg gccatctcca agtagccgcg acgttgacgg tgctggaggc
      481 cgactccaga caagcctttc cgagacggtg gacgagagtc cgacaccgcg agtttgtaag
      541 ccttacggcg ggcgcgggcg aggaccggat cgacatcgac caaaatcact aggtcgggtc
      601 tcaggccgcc cgccgcggcg cgcaggatcg gctgcgtgaa gtcacgcggc agatgccttc
      661 cgttctcacc catattctcg gcggtgaaca gaaaccgatc ggcgagtacc acgtcgctct
      721 ttcccaaggc cgggcgcagc gcctgttcga tcaactgcac gtctcgggcc acgtagagca
      781 gaaactctgc ttgcggcacc agctcgagat tccgcgcgtc gcgccccaaa tcccgaatgg
      841 cctccgacac ggacgacacg aactttcctt cggcgcgcag atgcttcacc gacagcccgc
      901 ccgcggctag ccgctcggcg acctggttgg aaaccgtggt tttcccactg ccatcgatac
      961 cttcgaaaac gacgaacatt accaagcgac tcccgcctgc aagagcaggc tttcgcgttc
     1021 cacgttgcta ttggggaaga aagtctccgg aaaattctcg ccggtacggt tgacctcacc
     1081 ttgcagcgtc aaccgagcac gtttccagaa tcccacattc atacctgcag ccgcttccca
     1141 cgccagatca ctacggatgt cgcggtcagc gtcgaagccg ccaaacatac cgaacggctc
     1201 cacgtaaaag tcgtcgtcct cgacgccgcc gaagcgcagc gccaccaggg cgcgaccctc
     1261 gacgaaaatc gggttttcgg tgctcgtctt atagcggtgc ttgtaccaac tctcaccggc
     1321 cactagttcg ctccaaacgc gtaagccgcc gtggggaagc tgtagatcca gagtcgcatc
     1381 gacgcctcca gtatgaaacc gatcactcac gcccagtgaa ggcgaaccaa cgcgatattc
     1441 gtaccaagcg cctaatccga tatgcagtat ttcaacacca gcacgcagga cataactttg
     1501 cgaatccatc gaaacggcgc cgatacccgt cgtgtctcga tttacgccgg caggcactcg
     1561 cctgagcgtg cgcccctgga agactgcggc ggtgaggctc ggacgaattc cgtgtttgtc
     1621 gcggacgccc accaggatgc cgggagagcg gccgccaatg tccagccaat cgtttagtaa
     1681 ccgatgcatg aagccgcggc gcacgatggg taggctccac ggcgactcga cctcgagcgg
     1741 tgagattggc attttgaact gccctgcgcg caccgtgaag tgctcgtcgc gagcctggat
     1801 gaacccgtcc ttgagcgaga tgccgtcgcc caactcgagc tcgacctgcg cagacagcca
     1861 aggcattgga gactcgtagt cgagcgccat gcgcgcgctg gcgagcgaaa gctgccaggc
     1921 gttgtacggc tcgaagccgc tcaagtctgg agtagcccgc cgacgtcgcg tgagctccga
     1981 caacgcgaag acccgccccc gaacttcgag gtggcccgtg ttcccaccgt gaaggatcga
     2041 gtttctgtgc ttcttgtgtt tttttgcggg gtgcttgtcg ccggatggcc gctcagtccc
     2101 atccgaggtc gttgcttcag aggcggcttg atcggcaggg tgcggctctt cacccgtcga
     2161 cgcgctctct tcggtgggag tctggtgggc ctccgccgca gccttggttc gcgcagcaga
     2221 gtccgtgggg cgcgatgcgg accgactgtc tccgctacgc ggctcgtcat cgttcgcagt
     2281 ctcaccctcg gcagatgtgg cgccgggctc gaccgcttcg gccacaccgg gcgcaaaaca
     2341 aagggacgag acgcacagac ccacgccacc gacaccataa cggcgaaggg tgacacgacc
     2401 ttgcgagcaa agcctaggca tcttggagtc cagagacctg ggaagacgca gccgggataa
     2461 acccagcttc ggcaagcgga ggatcatact cgctccgcgc gataatcgtt tagctttgcc
     2521 tgatagtcgg gtcgcgctgc ttgatagtca tgtcgcgctg ctcgatagtc atgtcgcgct
     2581 gctcgatggt tcgaggcact aggggctttc gttcgcgttg aggctggcat ccggggccga
     2641 ggaactaaga gggtcatgtt gagaatcgag gcgctcggct tgtacccgag ccgggctctt
     2701 ctttggagcc gccagcgtta aggtagttgc cggatggtgc gctattctgt cacttttatg
     2761 ttcgtgaatt ttaacactgc cgtcaccaac tgcgcggcgg acccaccctc accccgtaaa
     2821 cgcgaggatt ctgctggccg agaggaatgc ggtcaaggct gaatccaaac aggtggaaga
     2881 ggtgtcatgt gtgttacttt attgaaacca gcgtgagacc aggtctgccg actgctcagc
     2941 ccgaagttgc tccaaagcgc gctattttcg gggcatctgc cgcgatcgcg tgcatgctgc
     3001 tcgcgagctg caactcgaaa gatcacggag cgtccgactc ggccggtagc gcgaaacgca
     3061 cggcggcccg agccgtctcg aaagtcgata atacgagctc gggcggcaac tccgagaaca
     3121 cgcccaacac cactcgtcca ccaacgaatc cttcgtcgaa cggcggatcc ggaaccacag
     3181 aggcgggctc cacgtcgtcc ggcgccggtc caacgagcgg agagggcggt acctcgagct
     3241 cttcttcaac tctccct
//
LOCUS       DASTKU010000749         2470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_815625, whole genome shotgun sequence.
ACCESSION   DASTKU010000749 DASTKU010000000
VERSION     DASTKU010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815625"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VFQ61_26220"
     CDS             complement(<1..670)
                     /locus_tag="VFQ61_26220"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET9958032.1"
                     /translation="MSVRTPEALGTGFVVHPDGWIVTSLHVVGHGPEIVVTFEDGREL
                     PVIEVINGSRQHDLVILRVAAKGLPTLALGDSDRVRAGDSVVAIGHPLGLSDTVSNGL
                     VSAVRQLDSDLEVLQISAPIAPGSSGGPLFNEHGEVIGIATAILQGGQNLNLGLPAKY
                     VKRLMQTPEPISMPAFVAAMTQGDHEKQVPRHVPNHPVKLLRGCSSDNLSTISQSLGA
                     AIDVG"
     gene            complement(922..1536)
                     /locus_tag="VFQ61_26225"
     CDS             complement(922..1536)
                     /locus_tag="VFQ61_26225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958033.1"
                     /translation="MLTDILVNQSRDLLETQVERWGAEAKRLADTGHMVEALELYQKA
                     ADALPGAPWLQLRTAELARKLKRSDVAIMYFRRAADGFSRAGFAKRALPPRRSAWSVA
                     REGLPATQDVFVVISLELAKLQAQLGATTDAEFTIDHANDALRRAGIRHTIVWSESAS
                     TAPALTSSPPPASGPQTLPAGPETQPSGHGHSLAEASAAPRTQQ"
     gene            1740..>2470
                     /locus_tag="VFQ61_26230"
     CDS             1740..>2470
                     /locus_tag="VFQ61_26230"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET9958034.1"
                     /translation="MKTTEQLKADGSVSDVTSGSDSTPPSPAAAPNPGLVSRVVGCVL
                     GAAIGDALGHPTEFINSFDRLFERFGPQGVTGYELYWEHGGKRFAPYTDDTQMAELVL
                     RNLLWARESSADLERAMTELGVRFARWATHPQGGHRAPGNACLSGSYRLAEGVPWHEA
                     GGAQAGGCGSVMRVYPMGLLFSHESDELERWAVAQSKLTHRAPIALAACASMARGTSL
                     ALRGEQVPRILEEMVAAAARHDPGTA"
BASE COUNT          428 a          787 c          800 g          455 t
ORIGIN      
        1 ctccgacgtc gatcgcggcc cccaatgatt gactgatggt cgataagttg tcgctgctgc
       61 agccgcgcag caacttcaca gggtgattcg gtacgtgccg tgggacctgc ttttcgtgat
      121 cgccctgcgt catggcggcc acgaacgccg gcatcgagat cggctccggc gtctgcatga
      181 gccgcttcac gtacttggca ggtaggccca gattcaggtt ctgtccaccc tgaaggatag
      241 cggtcgcgat cccaattacc tcgccgtgtt cgttgaacaa tgggcccccg gatgagcctg
      301 gcgcaattgg cgccgagatc tggagcacct cgaggtcaga atcgagttgg cgcaccgcac
      361 tcacgagccc attcgagacc gtatccgaca ggcctagcgg gtgcccaatc gcaaccaccg
      421 agtcacccgc tcgcacccga tcgctgtcgc ccagcgcgag tgtggggagc cccttggcgg
      481 cgacgcgcaa gatcaccaaa tcgtgctgac ggctcccgtt gatcacttcg atcaccggca
      541 attcccgccc gtcttcgaag gtaacgacaa tctcggggcc gtgcccgacc acgtgaaggc
      601 tcgtcacgat ccagccgtcg ggatgcacca cgaagcccgt gccgagcgcc tcgggcgttc
      661 tcaccgacac gatcgccgcc gtggaccgat cggcgatctg tcgcggtgtg agtgatcgtt
      721 ccgcttgctc agcgggcttg tctggtgctg ccgcgggagc ttgtgcaccc gagtggcggc
      781 tacacgcggc ggagctccac accagcaccg ccaatccaag agcggtaagg gggcgaggga
      841 tacgagctgg ctccatccga cagattcgta agttagctca ggatcggcgc gcccgccgca
      901 ctccccgagc gagtaggacc ttcattgttg tgttcggggc gccgccgaag cttcggcgag
      961 tgagtgcccg tgcccggatg gttgcgtctc cgggccggcg ggtagagtct gaggtccaga
     1021 tgccggcggg ggggaagagg tgagggccgg ggcggttgag gcactttcgg accaaacgat
     1081 agtgtgccga attcccgccc tgcggagcgc gtcattcgcg tgatcgatcg tgaactccgc
     1141 gtcggtagtg gcaccaagtt gagcctgcaa tttcgcgagc tcgagcgaaa tcacgacgaa
     1201 cacgtcttgc gttgcgggca ggccttctcg cgctaccgac catgccgagc ggcgcggggg
     1261 gagggcgcgc ttagcgaagc cggcccgaga gaaaccatcc gccgcgcgac gaaaatacat
     1321 gattgcgaca tcactgcgtt tgagcttgcg cgcaagttcc gccgtacgca gctgcaacca
     1381 aggggctcca gggagagcgt ccgcggcctt ttgataaagc tcgagcgcct ccaccatatg
     1441 accggtgtct gcgagccgct tggcttccgc gccccaacgt tcgacctgtg tctctagtaa
     1501 gtctcgggat tggttcacga ggatgtcggt cagcacgcca cgtgccgagc ttcgacaccc
     1561 ggaaaacccc gtgtattctc agctttctcg agttcgccgc gtcccctgct gggacgttcg
     1621 acggttggac agtcccgaat cgggctcgtt cagcgcgagg ccgcccaggg cgccctcgat
     1681 gtagagcaca aggtgatacg ctcggcgcgc tcacagggac tagccgggaa ccagtaaaca
     1741 tgaaaacgac tgaacagctc aaagcggacg gatccgtctc cgacgtaacc tccgggagtg
     1801 attcaacgcc gccgagtcct gctgctgcgc cgaatccggg tcttgtctcc cgagtcgttg
     1861 gatgtgtgtt gggggccgcc atcggtgacg cgctcggaca tcctacggag ttcatcaact
     1921 cgttcgatcg gctcttcgaa cgcttcgggc cgcagggggt caccggctat gaactgtact
     1981 gggagcacgg cggcaaacgc ttcgctcctt acacggacga tactcagatg gccgagttgg
     2041 ttctgcgcaa cctgctttgg gcgcgcgaat ccagcgcgga tctggagcgc gccatgaccg
     2101 agctgggcgt gcgctttgcg cgttgggcca cccacccgca aggggggcat cgggcgcctg
     2161 gcaacgcttg cctgagcggt tcctatcggc tggcagaagg cgtcccgtgg cacgaagccg
     2221 gcggtgctca ggccggcggt tgcggctcgg tgatgcgcgt ctatccgatg ggcttgctct
     2281 tttctcacga gtcggacgag ctcgagcgct gggcggtggc tcaatccaag ctcacccacc
     2341 gcgctcccat cgcgctcgcc gcctgcgcga gcatggcacg cggaacgtcc ctcgcactgc
     2401 gcggtgaaca agtgcctcga atcttggagg agatggtcgc cgcagctgcc cgccatgatc
     2461 ccgggacggc
//
LOCUS       DASTKU010000750         6383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1487784, whole genome shotgun sequence.
ACCESSION   DASTKU010000750 DASTKU010000000
VERSION     DASTKU010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487784"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2937
                     /locus_tag="VFQ61_26235"
     CDS             <1..2937
                     /locus_tag="VFQ61_26235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9958035.1"
                     /translation="GKTRVLIGSAPHCEIRLAGPQVMPEHAAIVLQNGALSFVDAGIG
                     VSELNGHPLARGASAPFDFRSEFRVGHVPVPNLHPALALMLMSRGSAPFKPGELTFGR
                     EPARCNIAIAHPNVSGLHASFRLNPLSVVDHGSTSGTWLGQERLAPEAPRNVDVGAFV
                     ALGPVGIPVDLVLALAADFSAPAPGAAAPAPAGGNGAPDANRDGGAPRKHKTVIGQLQ
                     LGTPAQKPFMTIGRTPDNDIVIAQPQVSSRHAILHVQGSELAVEDRGSANGTFVRGQR
                     LTPGQRVPVQNGEKIFIGPMPLLIQSGNREVAVVVEDAAQWEGRPLYEIEAWDLVMQV
                     PDRDNPGELKTLLDHVSFKALPGDLIALMGPSGAGKTTLLLTLNGYLPPTAGQVRING
                     EDLYAIYDALRGSIGYVPQDDIVHPELTVFEAVRYSARFRLPPDYSDEEIDRRVEITL
                     AQLGLENVSHLEIGRPEKKILSGGQRKRVNIAMELVTDPVIMFLDEPTSGLAADDTAA
                     LIQLLADLAKKTGKTIITTIHQPAKDEFEKFNLALILGPGGLTTFFGPPKDGYRFFGS
                     WLERQGKPNNVDNPRDMFDMLNQRERPIFEAMRTQNPSSPRYMARAQAAREWNAEYFR
                     PENPVFQRMFSGKRAVGTGSTQQAVQSNRAQSSGQFGLLLSRYFKIKVRDVGGTAIML
                     LQAPIIGVLLALVFGGQKEAIPYWCLGALQELARKSGGLGEGIDQMLKNMATTPDHSG
                     AIFFLVVAAVWFGTSNAAREIVSERAIYMRERMVNLRLFNYVCSKFILLSFFCVIQCT
                     VLLAIVFFALGFNGGPRAFAFSLVTLIVTAMNSVAVGLLLSTLVTSAEAAMALTPIAL
                     IPQVVLGGLMVPMTTNSLLEWPMYLMPARWGFQGVVAQERLAIRNDAAWVLNLNRPDV
                     NSPENFVLAGKFHCAEAQIASLDFNGAWGFTNYEMVWLPPVVLTAMSLCAFVGILIIL
                     KRRDAI"
     gene            3321..5240
                     /locus_tag="VFQ61_26240"
     CDS             3321..5240
                     /locus_tag="VFQ61_26240"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HET9958036.1"
                     /translation="MPSLRLMFASAVPHEPCLGRSSSSTARSRPRHSSWLYTALTVVL
                     ACSDPASTNGTGVSPGTAGAGGRVGTTAGGSAGNGSGGAQSAGGSANSGGVLTASGGT
                     SNSGGAAPGAGTTSTGGAGPSTGGKPPGNGGSAGQGVGGAVGGNGGASANSGGRASGG
                     SAGATTNGGAPAGGATTGGGSNADVIAKFKNGVFWNDTQGNRIEAHGGGFYKEGDTWY
                     WIGEDKSHNSGNFKGVNLYSSKNLQDWQFRKAILTRTTTPTLDTADRIIERPKLTYNE
                     KTKQYVIWLHWEGKNYADAAAGVFKSANIEGPYTFVKSFRPKNNMSRDDNLFKDDDGK
                     AYFVSAANENADLILYELTEDYLDIQRQIVTLWKGSYREAPAIFKDNGRYFLVTSGAT
                     GWDPNQAKYATATSMDGPWTSLTNLGDSTTFDTQSTYVIPVQGSAATTYIFAADRWQD
                     PDLKGSKYIWMPLKVSGTSLSLAYYAEWQLNLTTGKWWVDDGYIPQGDWKLVRADSQE
                     TAGEDGKATNAFDDSPSTYWHTAWEEPGLKPPHELVIDLGATYDLKGFRYLPRQDKDD
                     HGMIAEYDFYASESPTDFGPPVASGTFGTSRQETKVSFSAKRARYVRLVAKSEINGND
                     WASVAELDLTGTRVN"
     gene            5643..6005
                     /locus_tag="VFQ61_26245"
     CDS             5643..6005
                     /locus_tag="VFQ61_26245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9958037.1"
                     /translation="MIDDEANTRVALDELLVDEGFDVETASDGVDGLAKIDTFQPHFV
                     LTDLRMPRMDGRELLSHILARTPHPAVFIMTGDTPSYASRWARDAVQYVSKPIDLGDL
                     VRRFDALLQRPELGAALG"
     gene            complement(6138..>6383)
                     /locus_tag="VFQ61_26250"
     CDS             complement(6138..>6383)
                     /locus_tag="VFQ61_26250"
                     /inference="COORDINATES: protein motif:HMM:NF015424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET9958038.1"
                     /translation="ILARDVSLSRERPAPTSILNLLQVRVLEISDDGPSRVNVQLSLL
                     DGSTRLLSRITRRSRAALALEPGTQVYAQVKSVALLA"
BASE COUNT         1185 a         2034 c         2037 g         1127 t
ORIGIN      
        1 ggaaaaactc gcgttctgat cggcagcgct ccccactgcg aaatccgctt ggcagggccg
       61 caagtcatgc ccgagcacgc ggccatcgtg ctgcagaatg gcgcgctctc gttcgtcgat
      121 gccggcatcg gcgtgagcga attgaacggt caccccttgg cgcgcggagc cagcgcgccg
      181 ttcgacttcc gctcggagtt ccgcgtcggc cacgttcccg taccgaacct gcaccccgcg
      241 ctcgcgttga tgctgatgtc ccggggttca gcgccgttca agcccgggga gctgacgttc
      301 gggcgtgaac ccgcgcgctg caacatcgcg atcgctcacc cgaacgtgtc cgggctccac
      361 gcgagcttcc gactcaaccc gctcagtgtg gtcgaccacg ggtccaccag cggaacctgg
      421 ctgggtcaag agcgcttggc cccggaggct ccgcggaacg tggacgtcgg ggccttcgtc
      481 gcgctgggcc ccgtcgggat cccggtggat ttggtgctgg cgttggcagc cgatttcagc
      541 gcgcccgctc caggcgcggc cgcgcccgcc cccgcgggtg ggaacggagc acccgacgcg
      601 aaccgagacg ggggggcccc gcgcaagcac aagaccgtga tcgggcagct ccaactcgga
      661 acccccgcgc agaagccgtt catgaccatc gggcgcacgc ccgacaacga catcgtcatc
      721 gcgcagcccc aagtctcgag ccgacacgcc atcttgcacg tgcagggctc ggagctcgcc
      781 gtggaagatc ggggcagcgc caacggcacc ttcgtacgtg ggcagcggct caccccgggg
      841 cagcgcgtgc ccgttcaaaa cggcgaaaag atcttcatcg gcccgatgcc gttgttgatc
      901 cagtctggca accgcgaggt tgcggtggtc gtcgaggacg ccgcgcagtg ggaaggtcgc
      961 ccgctgtacg aaatcgaagc ttgggacctg gtgatgcagg tccccgatcg cgataacccc
     1021 ggcgaactaa agacgctgct cgatcacgtc agcttcaagg cactgcccgg agacttgatc
     1081 gcgctcatgg gtccgagcgg cgccggcaag accacgttgc tgctcaccct gaacggttat
     1141 ttgccgccca ccgccggtca ggtccgcatc aacggcgagg atctgtacgc catttacgac
     1201 gccctacgcg gctcgatcgg ctacgtcccg caagacgaca tcgttcatcc cgaactcacc
     1261 gtgttcgaag ccgtgcgtta cagcgcgcgt tttcgcctgc cccccgatta ctcggacgaa
     1321 gagatcgatc gccgggtgga aatcacgctc gcgcagctcg gcctcgagaa cgtctcgcac
     1381 ctcgagattg gccggcccga aaagaagatc ttgtccggcg gtcagcgcaa gcgcgtcaac
     1441 atcgcgatgg agctcgtcac cgatccggtg atcatgttcc tcgacgagcc gaccagcggc
     1501 ttggccgccg acgacacggc cgccttgatc cagttactcg cagatctggc gaaaaagacc
     1561 ggcaaaacca tcatcactac catccatcag cccgccaagg acgagttcga gaagttcaac
     1621 ctcgccttga tcttgggccc cggcgggctg acgacgttct ttggtccgcc caaggatggc
     1681 tatcgattct tcggcagctg gctcgagcgc caaggcaagc cgaacaacgt cgacaatccg
     1741 cgcgacatgt tcgacatgct caatcagcgc gagcggccca tcttcgaagc catgcgcacg
     1801 caaaacccga gctcaccgcg ctacatggcg cgggcccagg ccgcgcgcga gtggaacgcc
     1861 gagtacttcc gccccgagaa cccggtcttt cagcgcatgt tctcgggcaa acgcgcggtt
     1921 ggcacgggct cgacgcagca agcggtgcag tccaatcgcg cccagagcag tggtcaattc
     1981 gggctcctgc tcagccgcta cttcaagatc aaggtccgcg acgtgggtgg cacggcgatc
     2041 atgctgctcc aagcacccat catcggtgtg ctgttggcct tggtgttcgg cgggcaaaaa
     2101 gaagccatcc cgtactggtg cctgggcgcg ctgcaagagc tcgcgcgcaa atcgggtggc
     2161 ctcggcgagg gcatcgacca gatgctcaaa aacatggcca ccactccgga tcattcgggg
     2221 gccatcttct tcttggtggt ggccgcggtt tggttcggca ccagcaacgc cgcccgtgaa
     2281 atcgtgagcg aacgcgcgat ctacatgcgc gagcggatgg tgaacctgcg tctgttcaac
     2341 tacgtctgct cgaaattcat cctgcttagt ttcttctgcg tgattcagtg cacggtgctg
     2401 ctggcgatcg tgttctttgc gctgggcttc aacggcgggc cgcgcgcatt cgccttctcg
     2461 ctcgtgacgc taatcgtgac cgccatgaac tccgtggcgg tgggcttgct tttgagtacg
     2521 ctcgttacga gcgccgaagc cgcgatggcc ctcactccga ttgcgctcat cccccaggtg
     2581 gtgctcggcg gtctgatggt gccgatgacg accaactcgc tgctcgagtg gcccatgtac
     2641 ctgatgccag cgcgttgggg cttccaagga gtcgtcgcgc aagagcgact cgcgatccgc
     2701 aatgatgcgg cgtgggtcct gaatctgaac cgtccggacg tgaacagccc ggagaacttc
     2761 gtgctcgcgg gcaagttcca ctgcgcggag gcccaaatcg cgagcctcga tttcaacggg
     2821 gcctggggct tcaccaacta cgagatggta tggctcccgc ccgtcgtgct cacggcgatg
     2881 agcctgtgcg cgttcgtcgg catcttgatc attctgaagc gacgcgacgc aatctgaggg
     2941 cctgcgaggg ggttattgcg ggggcaggcg cggttcgagg agagactgag gggggccttg
     3001 cctgcgctag agcgaaacgg gcagcgcttc gcgcacgtag acagcgcggc ccccctaacc
     3061 tctgaacacg agctcccgct cttccatagc ccgccggatt actgcgaaag tccaactctg
     3121 cgcctcgggg cacgctgctc tttgatggct tgatgctcgg gattggtccc taagtcggca
     3181 cccctcaatt agctcagacc aatacgctcg cggcgtgcta cgtaatttca aacttccaat
     3241 ttggcgggtg gtaccatgtc attcggtgat ggttttgggc ctgagcggag cgatgcgcac
     3301 gctatcctct cggcgtgccc atgccttccc tgaggttgat gtttgcgtcc gctgtgcccc
     3361 atgaaccctg cttggggcgc tcgtcgagca gcaccgctcg ctcgcgcccg aggcactcga
     3421 gttggctcta cacggcgctg actgtggtgc tcgcatgcag tgatccggcg agtacgaacg
     3481 gcaccggagt gtctccggga acggcgggtg cgggcggtcg cgtcggcacc actgcaggag
     3541 gcagcgccgg gaacgggagc ggtggcgcgc agagcgcggg gggcagcgcg aatagcggcg
     3601 gtgtgctcac tgcgagcggc ggcaccagca attcaggtgg tgccgcgcca ggggccggca
     3661 ccacgagcac gggcggagcg ggtccgagta cgggcgggaa gccgccgggc aacggcggct
     3721 cggcaggcca gggtgtcggc ggcgcagttg ggggtaacgg cggggccagc gcaaactccg
     3781 gtgggcgcgc atccggcggc agcgccggtg ccacgacgaa cggaggagca ccggccggcg
     3841 gtgccactac tggcggcggc tcgaacgccg atgtcatcgc caaattcaag aacggcgtgt
     3901 tttggaatga cacgcaaggc aaccgcatcg aagcgcatgg cggcggcttc tataaggaag
     3961 gcgacacttg gtattggatc ggcgaggata agtcgcacaa ctccggcaac ttcaagggcg
     4021 tcaacctcta ctcctcgaag aacctgcaag attggcaatt ccgcaaggcg atcctcaccc
     4081 gcaccacgac gccgacgcta gacaccgcgg atcgcatcat cgagcgtccg aagctcacgt
     4141 acaacgagaa gaccaagcag tacgtgattt ggctgcactg ggagggcaag aattacgcgg
     4201 acgcggcggc gggcgtgttc aagagcgcga acatcgaggg accgtatacg ttcgtcaagt
     4261 cgttccggcc gaaaaacaac atgtcgcgcg acgacaactt gttcaaagac gatgacggca
     4321 aggcttactt cgtgtcggcc gcaaacgaga acgccgactt gattctgtac gagctgaccg
     4381 aggattacct ggatattcag cgccaaatcg tgaccctctg gaagggtagc taccgtgagg
     4441 ccccggccat cttcaaagac aacggccgct atttcttggt gacctccggc gccacgggct
     4501 gggatccgaa ccaagcgaag tatgcaaccg cgaccagcat ggacgggccg tggacatcgc
     4561 tgaccaacct gggtgattcg acgaccttcg atacccaatc gacgtatgta atcccggtgc
     4621 agggcagcgc ggccacgact tacatctttg cggccgatcg ctggcaagat cccgatttga
     4681 agggctccaa gtacatttgg atgccgctga aggtgagcgg cacgagcttg agcttggcct
     4741 actacgcgga gtggcagctg aacctcacca cgggtaaatg gtgggtcgac gacggctata
     4801 tcccgcaagg ggactggaag ctggtgcgcg ccgatagtca ggagacggcg ggtgaagatg
     4861 gcaaagccac caatgccttc gatgactctc catccactta ctggcacacc gcgtgggagg
     4921 agccggggct caaaccgccc catgagctcg tgattgacct gggggcgacc tacgacttga
     4981 aagggttccg ttacttgccg cgccaggaca aggacgacca cggcatgatc gccgagtacg
     5041 acttttacgc gagcgaaagc ccgaccgact tcggtccccc cgtggcgagc ggcactttcg
     5101 gtacctcccg acaggaaacg aaggtcagtt tcagcgccaa gcgcgctcgg tacgtgcggc
     5161 tcgtcgccaa gagcgagatc aacggcaacg actgggcgag cgtcgccgag ttggatttaa
     5221 ccgggactcg cgtaaactaa gccactcgcg ccgcttccct cggtgacccc cgcgggtgtc
     5281 cgtttcgccc gagcgggaga aggacctcgg tccgcgaccc acgcgcatcg cattgggcct
     5341 acatcggacc ccgcgcagcc aagtctcagg cttgggctgc tttatctaag aaggtcctga
     5401 cctcgagctg gggctcacgt tacgccacac cctagcctac gtctcggacc cagcttgctc
     5461 caattctcga gccattgcgt cttctacagc ctggcgctcc ggcaaacctt gcgcagggct
     5521 ccccccaggt tcgcaggttc tgctcaacag ccaacgcaat tgcgccctcg caggtgccta
     5581 tttacgggca tggctcttgc tgaacaccgc agtggccgat gactacacga gcacgaatcc
     5641 tagtgatcga cgacgaagcg aacacacgtg tcgcgctcga cgagctccta gtcgatgaag
     5701 gcttcgacgt cgagaccgcc agcgacggtg ttgatggcct tgccaagatc gatacgtttc
     5761 agccccactt cgtgctgacg gatctgcgga tgccgcgcat ggacgggcgc gagctcctgt
     5821 cgcacatctt ggctcgcacc ccccatccgg cagtgttcat catgaccggc gacacgccgt
     5881 catacgccag tcggtgggcg cgcgacgccg ttcagtacgt ctccaagccg attgatctgg
     5941 gcgatctggt gcggcgcttt gacgctttgc tgcagcgccc ggagctcggc gccgccctgg
     6001 gctgagcgcg ttcatgcgac gggcgggcgc ggtgttgcga gcgctcgtgg gctcgcacga
     6061 cggagctgga gcgggatttc gccgaggagt cgcgcacgtt gttcggcttg ccccagccaa
     6121 ggattcgagc ttttccgtca cgcgagcagc gcgacgctct ttacctgcgc gtaaacctgc
     6181 gtacccggtt cgagcgcgag cgccgcgcgc gaacggcgcg tgatgcgcga gagtaagcga
     6241 gtggagccgt cgaggaggct gagttgaacg ttgacgcggc tcggaccgtc gtcggagatc
     6301 tcgaggacgc ggacctgcag gaggttcaag atgctggtcg gcgccggccg ttcccgggag
     6361 aggctcacgt cccgcgccaa gat
//
LOCUS       DASTKU010000751         3324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_593549, whole genome shotgun sequence.
ACCESSION   DASTKU010000751 DASTKU010000000
VERSION     DASTKU010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593549"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..560)
                     /locus_tag="VFQ61_26255"
     CDS             complement(342..560)
                     /locus_tag="VFQ61_26255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958039.1"
                     /translation="MLKEPTLERLQALRLLAMATAWDEQQRHPDIQSMTFDERFALLV
                     EAGKGIRSTTAEKLGGSDPQCPLSPNER"
     gene            complement(572..2088)
                     /gene="istA"
                     /locus_tag="VFQ61_26260"
                     /pseudo
     CDS             complement(572..2088)
                     /gene="istA"
                     /locus_tag="VFQ61_26260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213865.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          589 a          995 c         1107 g          633 t
ORIGIN      
        1 gctgcgatgg acggctgcga tgtgttcccg aaccagctgc tcgatgcgat ggtccaagtc
       61 tcgatggcct gcggtgtctc tcatgccgct cgcttctcat gcggcacacc gaacctcaac
      121 tcaccccgtg aacgggtacg ctcagcccag cgctacggac tcgccgtctc tcagcacccg
      181 ttcctctcga ccgagctcct cgaggtcgag ccggcaggcg accacatagt tgcggtcgtt
      241 tacgacctag aaattaccaa ggtcgtgacg ccaacggctg ttggtccggg tctcgtgcaa
      301 gcgctggacc tcacgggcga gtctgcggtt gatacgagcg atcagcgctc gtttggtgac
      361 aatggacatt ggggatcact tcctccgagt ttctcggctg ttgttgatcg aatgcctttg
      421 cccgcttcga cgagcaacgc gaagcgttcg tcgaaagtca tggactggat gtcggggtgg
      481 cgttgctgct catcccaggc agtggccatc gccaggaggc gaagcgcttg gagtctttcg
      541 agagttggtt ctttgagcat ttcgatttcc ttcagtgata gtagtcgcgc ccacgaacat
      601 tctcgtgggt gacgctgggg ccgcgcggct cgatgtcgag cgcgggggcg cgatcgagtc
      661 cgtgctggag gatggaggcg acgtgtcggt aggagcaggc gccgactccg aaggcgcgct
      721 cgcacgctgc ctccacgcga tcggctccgt acttcttcga aagacggaac acgcccagac
      781 acgagcggta gccgtgctcg gggtgagtcc gctgggcgag caggcgttcg gcaaagcgcc
      841 gcgtgtagac gccgacgttg gcggcccatc ccaggatgcg ctccggggtc cactcggcat
      901 gctgctaatg agcgagaggc atgtgtgcgg gcacggtcgt atgccgcccc tgaacgcgac
      961 tccgcacatg agccgcttcg cggcgtccga gcctgaaaat ctcgatggtc gtggcggtgg
     1021 cacgaagcca cagctcttcg cctcgaagcc cgtgcggcgc cgagtagtag tgcccctcga
     1081 actccgcgtg atagtcgatg ccgagcgtga cccgcttcca ctcggcgtac tcgtacggcg
     1141 agagcggcag tggacggagc gcggggcgat cgagtcgctc gaacagctcg cgacggctcg
     1201 cctgatacgt gcgcatctgg cgatcgttga gctcgctgca gagctcggcg atgcgtgcgt
     1261 tgagctcggc gagggagaag aaggtctggt tgcggagacg tgcgaggatc caacgctgag
     1321 cgatctgcac ggcgacttcg actttggatt tgtcgcgggg cttgcggggt cgcgcaggaa
     1381 agatcgtggt catatggtgg cgcgcccact cgtcgaaggt cttttgcacg ccgggctcgt
     1441 acttgcacgc cagggtcacc gcggacttga gctgatcggg gacgatggca ttcggcacgc
     1501 cacccatgaa cgcgagcgcg cgcgtgttgc tcgcgagcca gtctggcaac tgctggctgt
     1561 acgtggcctc ggcgaacgtg tagttggacg cgcccagcac ggcgacgaac agctcggcct
     1621 cgatcacttc tcccgtaagg gggtcgacga tgtgcggctt ctttccggag tagtcgacga
     1681 acagcttgtc acccgcgaga tgcgtttgcc gcatcgagat gcgctgcttg cggacccact
     1741 cgcggtagcg ctgacagaac accgtgtagc ggtagccgtc cggatgttgc tcgagatact
     1801 cgagatgcag cagcgacagc gtcacgcccg tgcgccgcag ctcgacgtgg atatagggca
     1861 ggtcgggctc ggcgcggcca tcgcgcttgc ctccacgcgg cccgtacatg cgcgtctcga
     1921 gctgctcttc gtcgagcttc tccaccgctt cccagtcgag gccgagggcc ttggcgcgaa
     1981 tgcagcactc gccacggagc ccgcgctgat gcgcacgacc tctgccgttt ttcggtggct
     2041 gcgacccagc tgcagcttct gtcgcaagat ctctcgtagg tccctcatcg tcagtctctc
     2101 tgtcgccacg cgctccgtgc ggaggcgcgg gggtgggggg aaacgaacgt cgggacgccg
     2161 agcctcgcga ctccgaggat cgtgcaaaac tgaacggacg gacagtccga tcaggagctc
     2221 gcggaggccg cgatcacgat ggcgtaatcg gcgatcacca tcgcgaaaga ggtgatcacg
     2281 atgccgcgaa acgggtgatc acgatggcgc gtaatcggcg atcacgatgg cgcgaaacac
     2341 gcaatctagc aatcgaagca agtaattcga cattaccgcg tcacgccagc cgtttgcccc
     2401 atggcgatgt tgacctgggg aatgctggcg tctggtaaga cgaggcagat ggcgcggcgg
     2461 ctcgtggatc cgccatggcc gatgacatcg gttcaatccc gcgcgcgacg gtgccgcagg
     2521 aaagcgcgag ggtcattgag caggcgtcag cacgcgaaat tatgcaggtc ttggccaaac
     2581 tcgttgtcag cgctatcgtt cacgaatgtc ttcccatccc gctcctgaca cacttccggg
     2641 gctctggaag ccccacccgg atgacgaggc cgagatcggc gaggcgatgc aggcagccac
     2701 tcagctcgag cttctctcgc cagaggcgtc agagaggttt attcggtgga tggagggagc
     2761 gggcgacgaa gcgtggcgcg tcgagctcga gtaaccacgt ggtacgctcg ccaggttggc
     2821 cgcttcatca gtcctgggac ggagcgggcg cttgccgtcg cgcgtacgat ccgcgctctg
     2881 gcggcagcgg aggaactgcc gggagcctct gatttcgagg ggaccctgaa aactgttgga
     2941 cgggcgtggg tccggagggt caaaggccgg aatctctggc tgtggtaccg gttcgatgac
     3001 gctgaggtca tgttcattgc ggtcacgacc gagcctcctg tgcctctcga tgacgacccg
     3061 aaggtggagt gaacgccggg gaaagaaccc tgccggaagt gcatcccaac tcacagtgtg
     3121 ctcacccgcg ggtgagcaca ctgagacggt ttcgagaggc gcgcagcggc acagcaaaaa
     3181 tggcctccga cacggcgggg aacgtgtcgg cacggtaaaa tttcactttg ccaaggttga
     3241 cgtcgagagt tcgaatctct tctcccgctc tcagttttct ggctgctggg caccccaacc
     3301 cagcaggatt tgatgttcct gctt
//
LOCUS       DASTKU010000752         7557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_369970, whole genome shotgun sequence.
ACCESSION   DASTKU010000752 DASTKU010000000
VERSION     DASTKU010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369970"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFQ61_26265"
     CDS             <1..432
                     /locus_tag="VFQ61_26265"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9958040.1"
                     /translation="SMGQAAAGVIGAALGSGRKAVAIVGDGSMLMLNEINTAASYGID
                     ALWIVLNDARYAMIAQGMESIGWTPFETDFPRADFAAIARAMGAHGARVEYESQLEAA
                     LERALSARGPYLLDVMIDPREKAPSNRRNRSLVAQGVKGGP"
     gene            429..1136
                     /locus_tag="VFQ61_26270"
     CDS             429..1136
                     /locus_tag="VFQ61_26270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958041.1"
                     /translation="MNAVPSRAAAAFIGPLELQSFPERISARVVKADAEPRVHGYSVQ
                     GDLARYYGVADLWLLCLTGELPEPALARAFSVACWFLAPVSVADASVHAAVLARLCGT
                     PPSATIATAALSLAEQARFEVESLGDLLEFLSSRSGELPAHHRAQTPAQCQELEPLLA
                     ALRLNAAPFPEIGHRPTLTAALFAILFECGLKSSEQLQAALVFARLPIATAEAFAEQR
                     CNFRQYPANLPRYEYEP"
     gene            1133..1975
                     /locus_tag="VFQ61_26275"
     CDS             1133..1975
                     /locus_tag="VFQ61_26275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958042.1"
                     /translation="MSSQATGSQPLRPLPCKTRCAHWGHTEHQYFGHAVFSQLLEHES
                     LMGLNALSVLGRRLTDEQCHVLEEAALTLTMADPRIWPLKLTRVVAAYGRSVPAAAAG
                     ILVLQDARIGPWPTVQTSTMLKAFHERWQGRWERQSVHADVRAYLNEHGFLWGFGTPF
                     RTRDERLLAFRPCIERRGLHRYPHFMTFEALCEAAREQCDAPPNILCALAAVLLDMGI
                     SCAETGPVVTVLMQHMFLANAVEAAAESPSLLRKLPKERVEYCGPPDRKSPHGLRSAT
                     ERVR"
     gene            2169..2636
                     /locus_tag="VFQ61_26280"
     CDS             2169..2636
                     /locus_tag="VFQ61_26280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958043.1"
                     /translation="MACEIIATDRAVFALWGKPEKADFDRLLDTLQSVTLRTGTPPIY
                     VTRVPGDDPAPSAEERAYLDRLMPKFVELCSTYHVILEGAGFMAALKRGVLVSLFQIR
                     WRRGTFFVHAHSSEVPACLPLLQQQKAASELLRLARARGLLAGEKTEAGASAA"
     gene            complement(2707..3600)
                     /locus_tag="VFQ61_26285"
     CDS             complement(2707..3600)
                     /locus_tag="VFQ61_26285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958044.1"
                     /translation="MMHSESSVARSAVLLALVFAGLSWRARPARAQETAQACASAYEE
                     AQRLRARGALKQASHEAQVCSQVECNQFIVQECIKLYEQIQADTPSMIFTARNGDGEE
                     LNAVQVQVDGQVVAERLDGRPIDLDPGPHTFRFEAAGLPPLETKHTARVGDRNRVIEV
                     MLGEKRAPQPPPPALAPTSPLLPPQGGTRHAQVPVGTWVLGGLGIVGLGAFGYLRLAA
                     TNEYNDFATTCSPRCDPKETDKVHTKFQLSYVALGVGAVSLGGAALVYVLTREKDGAR
                     ESSVALVPTEDGLRAGWRTRF"
     gene            complement(3628..5028)
                     /locus_tag="VFQ61_26290"
     CDS             complement(3628..5028)
                     /locus_tag="VFQ61_26290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958045.1"
                     /translation="MLRLSPLALCLGSGLLLLAACEPDLDALTREYATASGGQSGSGG
                     SGGTSSGGRESGGTSQSGGTGGQTVKPDTCTDQRLSPNESDVDCGGSSACQRCELNAR
                     CTRHTDCETGICARGRCSEPTCTDGVQNQDETGVDCGGSCAPDKLCEDGLACGVHEDC
                     QSGFCNQKVCDDHCASKRLDGDETDVDCGGESCDRCPNDSACLMVSDCESGVCKNNRC
                     VDPSCDDEVKNQDETDIDCGGVCRTTRPCGINDTCKIGADCSSYVCSERCVADITIPT
                     QDMIDNFEDQDLLLTRVNGRVGSWSPFGDGSDGSAAELKTEPIPGRRAASAQALHYSG
                     AGFTDWGSGFGFDFSNNSSNENTKKPWNASTYKGVTFWAKSANDSSFTLQLLDRNTHV
                     AGGVCQTCECAWGKSLELSSEWQRYIVLFSELTLSSGCDPVPTAFDKSGVVMMQLLFS
                     KNATADLEIDDVALLR"
     gene            5681..6991
                     /locus_tag="VFQ61_26295"
     CDS             5681..6991
                     /locus_tag="VFQ61_26295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958046.1"
                     /translation="MSPIGSVASSPTPDSKPSLLERAPEPKPLEPDGNDEGEEPGSSN
                     LTGPAHFELVGQPPLALQRICDLTVIADQLFATHAVSALGSDGATVSRFRADQPKTPF
                     QVAFDWNRPGEPSRGGGAGQGFLRARALSGRLFVPDADPPYYGFWLSHTSPEGFVFVS
                     DSQGDFARARRPGYLPPAPPSETSKSGAGILPGAYHVLDVIRFRDHYYASTGAVPRGG
                     HANQAEAPGVLYVADPGFAHWQHAAEYPHPAVPGVTRLTFLVRFRDRLYAGVQDFDAR
                     GGIDYVYWAPPPDVSELRPEDMHAVRATEDGAAATYRWYADRGQLYWISATRSGVALR
                     RTADGEHWIHVPLPSEFGPPTDIVRFGPELFVLTEWALLRLDERGAHPVAHVRTKRSP
                     FQLGDYFCAAPLVVYRNQIYAGGQDRGRLYRVVEGDAPADPTPK"
     gene            7147..7530
                     /locus_tag="VFQ61_26300"
     CDS             7147..7530
                     /locus_tag="VFQ61_26300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958047.1"
                     /translation="MTLPAERSPQIANPFVSLAARIVELRERVEDAAWIPASEQTHQS
                     ATQFVVDAFEILAQFRESAVQQSDLQRAESVATRELTAAKRHLELAKRSPETRSAACD
                     LAVRALTRAVDSLLNALVPHTIPPG"
BASE COUNT         1398 a         2491 c         2280 g         1388 t
ORIGIN      
        1 tcgatgggcc aggccgcggc gggggtgatc ggggccgcgc tcggtagcgg gcgcaaggcc
       61 gtcgcaatcg tgggcgacgg ctcgatgctg atgctgaacg agatcaatac cgcagcttcc
      121 tacggaatcg acgcgctgtg gatcgtcctg aacgacgcgc gatatgcgat gatcgctcag
      181 ggcatggaat cgattggttg gacgccgttc gagaccgact tcccgagggc agacttcgcg
      241 gcaatcgccc gcgccatggg cgcgcacggc gcgcgcgtcg aatacgaaag ccagctcgaa
      301 gccgcgctcg agcgggccct gtcggcgcgg ggaccatatc tcctcgacgt gatgatcgat
      361 ccaagagaaa aagccccttc caatcgccgc aatcgaagcc tcgtcgcgca gggcgtgaaa
      421 ggtggcccat gaacgccgta ccctcgagag cagccgcggc tttcattgga ccgctcgagc
      481 tgcagagctt cccggagcgg atctccgcgc gggtggtcaa agccgacgcg gagccgcgcg
      541 tccacggcta ctccgttcaa ggagacttgg cgaggtacta cggggtcgca gatctttggc
      601 tgctgtgtct gaccggcgag ctccccgagc cagccctggc ccgcgccttc agtgtggcct
      661 gctggtttct tgcacccgtg tcggtcgcgg acgcatccgt gcacgcagcc gtgttggcac
      721 gcctgtgcgg cacgccgcca agcgcaacaa tcgcgacggc agcgctatcc ctagccgaac
      781 aagcgcgctt cgaagtggag tccttgggtg acctgttgga gtttttgagc tcgcgttcgg
      841 gagagctgcc cgcgcatcac cgggcccaga ctcccgcgca atgccaggag ctcgagccgc
      901 tcctggccgc tctgcgcctg aacgccgcac cttttcctga aatcgggcac cgtcccaccc
      961 tcaccgccgc tctgtttgcc atcctgttcg agtgcggctt gaagtcgagt gaacagctcc
     1021 aagcggcgct ggtgtttgcg cgcttaccaa ttgctactgc ggaagccttc gccgaacagc
     1081 gctgtaactt caggcagtat cccgccaacc taccgcgtta cgagtacgag ccatgagctc
     1141 ccaagccacc ggctcccagc cgctgcgtcc actgccgtgc aaaactcgct gtgcgcactg
     1201 gggccacacg gagcatcaat acttcggcca tgcggtcttc tctcaattgc tcgaacacga
     1261 gagcttgatg gggttgaacg ctctctcggt gctggggcgg cgattgaccg acgagcaatg
     1321 ccacgtgctc gaggaggcag cgctgaccct caccatggcc gacccacgaa tttggccact
     1381 caagctgaca cgagtcgtgg ccgcttacgg gcgctccgtt ccggccgcgg ccgcagggat
     1441 cttggtctta caggatgctc gaatcggtcc gtggccaacg gttcagactt caacgatgtt
     1501 gaaggccttc catgagcgct ggcagggcag gtgggagcga cagagcgtgc acgcggacgt
     1561 gcgtgcctac ctgaatgagc atgggttcct ttgggggttc ggcactcctt tccgcacacg
     1621 cgatgagcgc ctgctcgcgt ttcgcccgtg tatcgagcgc cgtggactgc accggtaccc
     1681 gcacttcatg actttcgagg ctctgtgcga agcggcccgc gaacaatgtg acgcgccacc
     1741 caatattctg tgcgccctcg cggcggtgtt actcgacatg gggattagct gcgctgaaac
     1801 aggccccgta gtcaccgtgc taatgcaaca catgttcctt gccaatgcgg tcgaggccgc
     1861 cgcggaaagc ccaagtctgc tcagaaaatt gccgaaagag cgcgtcgagt actgcggccc
     1921 accagaccgc aagagcccac atgggttgcg atccgcgacc gagcgcgtga ggtgagcagc
     1981 tcctgcgcgg cgcgatgtct ggagcgtgcg ttggctatcg cacgagattg gcgtccttcg
     2041 tcgagacgcg atcggaaatc gactcaagtc ggcgacgggc tagccgttgc tgttgggcgg
     2101 gggacgcgac cgagagcatt gagcatcttc tcaacgcaac tctgcgttcg cactgggact
     2161 cgaaagcgat ggcttgtgag atcatagcga cggaccgagc agtcttcgcc ctttggggca
     2221 agccagagaa ggccgacttc gaccggctac tcgacacatt gcagtcggtg actctacgca
     2281 cgggcacccc acccatttac gttacgcgcg tgccgggcga cgatcccgca cccagcgcgg
     2341 aagagcgtgc ctatctcgat cggttgatgc ccaagttcgt cgaactctgc tcgacctacc
     2401 acgtcatcct cgaaggagcg ggcttcatgg ccgcgctcaa gcgcggcgtg ttggtcagcc
     2461 tgtttcagat tcgttggcgc cggggcacgt tcttcgtaca cgcgcacagc agtgaagtgc
     2521 ccgcttgcct gcccctgttg cagcagcaga aggcggcaag cgagctccta cgcctggcgc
     2581 gcgcgagagg cctacttgcg ggcgagaaaa ccgaagccgg cgcgagcgcc gcttagcgcc
     2641 caagccgcct ccggcgcctc gtcagtcgca gtctctcgag ttgaatcggg gtcgagacgg
     2701 gcaggcctag aagcgagtcc gccaaccggc gcgtaagccg tcttcggtcg gaacgagtgc
     2761 gacgctcgac tctcgagcgc catccttctc gcgagtcaga acgtacacca acgcggctcc
     2821 gccgaggcta acggcgccga ctcccagcgc cacgtaagac agctgaaact tggtgtgaac
     2881 tttatctgtc tctttcgggt cgcatcgagg gctgcaggtg gtcgcgaagt cgttgtactc
     2941 gttggttgcg gcgagacgca aatagccaaa cgcgcccaag ccgacgatac ccagcccacc
     3001 gagtacccac gtgcccaccg ggacttgggc gtgcctggtt ccgccctgag gaggcagcag
     3061 ggggctcgtc ggcgccagcg caggaggcgg gggttgcggc gcgcgcttct cacccagcat
     3121 cacttcgatg actcgatttc ggtctccaac gcgcgcggtg tgtttcgttt ccaacggcgg
     3181 cagccccgcg gcttcgaaac ggaaggtgtg gggccccgga tccagatcga tgggccggcc
     3241 atcgaggcgc tcggcgacga cctggccatc gacctgcact tgcaccgcgt tcaactcttc
     3301 accatcgccg tttcgcgcgg tgaagatcat cgagggcgtg tcggcctgaa tttgctcata
     3361 gagcttgatg cactcctgaa cgataaattg gttgcattcg acctgagagc atacctgggc
     3421 ttcgtggctc gcctgtttga gcgctccgcg cgcgcgtaag cgctgggcct cttcataggc
     3481 ggaggcgcag gcttgcgccg tctcctgggc acgggccggg cgggctcgcc aagacaggcc
     3541 agcaaacacc aaagctaata aaactgccga gcgagcgaca ctgctctcac tatgcatcat
     3601 ggactgcctc gcttcatgga ctaagcctca cctcaacaac gcaacgtcgt caatttctaa
     3661 gtcggccgtg gcgttcttgg aaaacaacag ttgcatcatc accacgccgc tcttgtcgaa
     3721 cgcggtcggg accggatcac agccggagct cagcgtgagc tccgagaaca acactatata
     3781 ccgttgccat tcggacgata actccaggct cttgccccaa gcgcattcgc acgtttgaca
     3841 cacgccacca gcgacgtgcg tattgcgatc taggagctgc agcgtgaacg aggagtcatt
     3901 ggcggacttc gcccagaacg tgacgccctt gtaggtcgag gcgttccaag gcttcttggt
     3961 attttcattc gacgaattgt tcgaaaaatc gaagccaaac cccgatcccc agtccgtgaa
     4021 cccagccccc gagtaatgca gggcttgtgc ggacgcagcc cggcggcccg ggattggctc
     4081 cgtcttgagt tcggccgcgc tgccatcgct gccgtccccg aaaggagacc agctgccgac
     4141 gcgcccgttg acccgcgtca agagcagatc ttgatcttcg aagttgtcga tcatgtcctg
     4201 agtaggaatc gtgatatccg ccacgcagcg ctcggagcac acgtagctcg agcagtctgc
     4261 cccaatcttg caggtgtcgt tgattccgca gggccgcgtg gttcggcaga ctccgccgca
     4321 gtcgatgtcc gtttcgtctt ggtttttgac ctcgtcatcg cacgaagggt ccacgcagcg
     4381 gttgttctta caaaccccgc tctcgcaatc agagaccatc agacaagccg agtcattcgg
     4441 acaacgatcg cagctctccc cgccacaatc gacgtccgtc tcgtcgccat cgagccgctt
     4501 gctcgcgcag tgatcgtcgc acaccttctg gttgcaaaac ccgctttggc agtcctcgtg
     4561 cacgccgcaa gccaggccgt cttcgcaaag cttgtccggc gcgcagctgc cgccgcaatc
     4621 gacgcccgtt tcgtcctgat tttgaacgcc atcggtgcag gtcggttcgg agcagcgacc
     4681 gcgagcacag atgccggttt cgcagtccgt atgccgagtg cagcgcgcgt tcagctcaca
     4741 gcgttggcac gcgctactgc caccgcaatc cacgtccgac tcgttgggac tgagccgttg
     4801 gtcggtgcag gtatccggct tcaccgtttg ccctcccgtt cccccggact gagatgtccc
     4861 tcccgattct ctaccacccg agctcgtgcc accgctgccc ccggacccgc tttggcctcc
     4921 gctcgcggtc gcatactcgc gggtcaacgc atccagatcc ggctcgcacg cggcgagtag
     4981 cagcaaaccg gaacctaggc acagagcgag aggactcaga cgcaacatcg tgaacgacca
     5041 ttcggttcga cgaacagagc cgacgcagcg tcagtccggc gtttctcccg tgccgtaacc
     5101 gatctgcaaa aagctcacca aagagagagg acgatctgcg atcccgcact atccaagcag
     5161 agtgagcgca gatattccac cagatttgct gtcgacgccg agaagcctcc tacaggcttc
     5221 ctcgaccccg catcgcggcg ccgggggaga gacacgccca attggggaga gacgccgctt
     5281 gagatccggg cacgaccccg aagcgggcac tgacacggag ccgatctatc cggccttgta
     5341 ccggctccca caaccgatca ccgagcggca ccgcgagccc gtgtgcacta gagtggccgg
     5401 gcacagcgcg gccggagccg gcacggcttc gcccaatgag cttacacggt ggatccccga
     5461 atgaacggag caaaagccca tgcagcctga cgctcgtcag agtaggactc cacacgggcc
     5521 cagctcgcga aaactcgtta ccgcgctaat cggggtgtcg ctcgctgcga gctgccgctc
     5581 tcctgccctg gattccgggg tcaagccgac cgacgtcagc cgagccgctg gatccacttc
     5641 ctcgggaaat gagcggccta atccaaagct cccgtcgacg atgtcgccaa tcggctccgt
     5701 cgcatcttca cctacacccg attcgaaacc gagcctcctc gagcgcgccc cggagccgaa
     5761 gccgctcgag cctgacggca acgatgaagg ggaggagcca ggctcgtcga atctgacggg
     5821 cccagcccac ttcgaactcg tgggccagcc gccgctcgcg ctgcagcgca tttgtgactt
     5881 gaccgtcatc gccgatcaac tgttcgcgac ccacgccgtg tcggcgctgg gcagcgacgg
     5941 agctacggtc tcccgctttc gagcggatca gcccaagacg ccattccagg tcgcattcga
     6001 ttggaatcgt cctggcgaac ccagccgtgg gggtggcgcg ggccaggggt ttttgcgcgc
     6061 ccgggccttg agcggtcggc tcttcgtacc cgacgcagac cctccctact acggcttctg
     6121 gctgtcccac accagcccag aagggttcgt gttcgtctcg gactcacagg gcgacttcgc
     6181 gcgagcccgt cgccccggat atcttccacc ggcccctccg agcgaaacga gcaaaagcgg
     6241 tgctggtata ttgcccggcg cttatcacgt gctcgatgtg attcgctttc gggatcacta
     6301 ctatgcatcg acgggtgcag tgcctcgggg tggtcacgcc aaccaggcgg aggcgccagg
     6361 ggttctttac gtggctgacc ctggttttgc gcattggcag catgccgcgg aataccctca
     6421 tccggccgtg ccgggggtca ctcgactcac gttcctggtc cgctttcgag atcggttgta
     6481 tgccggggtt caggacttcg atgcgcgagg cggcatcgac tatgtctatt gggctccacc
     6541 tccggacgtc agcgaacttc gtccagaaga catgcatgcc gtgcgagcca ccgaagacgg
     6601 agccgcggcg acatatcgct ggtatgccga tcgcgggcaa ctatattgga tctccgcgac
     6661 acgaagtggc gtcgcgctca gacgaaccgc cgacggagag cactggatac acgtacccct
     6721 gccctcggaa ttcggccctc ctaccgacat cgtgcgcttt ggcccagagc tgttcgtact
     6781 gacggagtgg gccctcctgc gcctggacga gcgcggcgcc caccccgtgg cccacgttcg
     6841 aaccaaacga tctccgttcc agctagggga ctacttctgc gctgcgccct tggtggttta
     6901 ccggaaccag atctatgcgg gagggcagga ccgtggtcga ttgtatcgag tggtggaagg
     6961 cgacgccccc gcggatccaa caccgaagtg agatcgctga ttctctaagc gagagcgtcg
     7021 aggcaagccg cgaatcgtcg cgtcgagcag aaccgctaga ttatcgagaa atggagctga
     7081 ctacgcgcaa ccgcgcgccg cgcgccgcgc gcttcgcgtg gcacgttcaa tgctccaacg
     7141 tggcccatga ccctccccgc tgaacgcagc cctcaaatcg cgaatccgtt cgtgtcactc
     7201 gcggcgcgca tcgtggagct ccgcgaacgt gtggaagacg ccgcctggat ccctgcatcg
     7261 gagcagactc accaatcggc gacgcaattc gtggtcgacg ccttcgaaat cctcgcccaa
     7321 ttccgggaga gcgcggtcca gcagagcgac ctacaacgag cggagagcgt cgccacccgc
     7381 gaactcaccg ccgccaagcg ccacttggag ctcgcgaaac gtagcccgga gacccgtagc
     7441 gccgcctgcg atcttgcggt tcgagcgctc acccgcgcag tcgattctct gctcaatgcg
     7501 cttgtcccac atacgatccc gcctggttga taaccaccct gtagttacca accctgt
//
LOCUS       DASTKU010000753         6480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1710948, whole genome shotgun sequence.
ACCESSION   DASTKU010000753 DASTKU010000000
VERSION     DASTKU010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710948"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="VFQ61_26305"
     CDS             <1..1381
                     /locus_tag="VFQ61_26305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958048.1"
                     /translation="SEPEHSDPQGQVPDSEALTARTPAYGSKPQAVPEAALIEALGCE
                     PSVPEPPTASPPLVALAQSATSTRTSSAKGVHGTFPERQKNPKSPTARALGEALAELA
                     EQAREEPVAVRTEAPTALGDIADEVLRSLTVQDEPVASNRSEKQVEELSSLAALVTEA
                     PQRDLPESTATRAPELPPLYPNAPSEWRNWLRDLEAARGPKPLSAIERMFITSYLPLS
                     YACVHGYADPAAERALENWSQSFAQSYSEAFDALRVRGKRPSMVLDAPELALRIGRLH
                     GARSVQLLLVDGMRFDLGLLVEQRVRLALGQDAALTERLLLWAALPTTSEVQLDLLGR
                     GPEALKEGRTSGGSDVTVARGRAAATLRRVKTHNRELLKLDLVEARLAEPGPREAQRL
                     SSLADETAAILVEHISKQAPRTLVFAFGDHGFRLDPLEKGTRAAQSGGATPEEVLVPA
                     FAWLVGGVH"
     gene            complement(1465..3312)
                     /locus_tag="VFQ61_26310"
     CDS             complement(1465..3312)
                     /locus_tag="VFQ61_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9958049.1"
                     /translation="MATIRYFSNDGNQVLLALHKPVTTVGRALGNDVQIPDASVAAHH
                     IQIVFNGRDFQLEEVDREAEIWINGKKKRRARLCNGDRITLGKAQLAFSMFSEDAGEP
                     VSDTGASEMQSDLSGLRRLQTFSERLMTTRSVDELLETLLDDVIELTGAARGWVLWVD
                     PGDSKDVLPRVRASRNVQREAIQDSSGLMSDSIVRKVIETKRAVIVSDALNDTTFGKS
                     ESVIALKLSSVMCVPLLGQGEIVGALYVANDEVKHLFDRRKLDLLTVYASQASLILQN
                     AMLLSALRADKEKLSAELSDKRFGEIIGACPSMMEVFRKLQKVASTDISVLITGETGT
                     GKELIARELHRRSSRANGPFVTVNCGAIPENLIESEMFGHVKGAFTGAIASRPGKFQQ
                     ADGGTLFLDEVGELPAQLQVKLLRALQERVVIRVGDSRPEKCDIRIVSATNRNLDEMI
                     KTGEFREDLYYRLNVVNLWLPPLRDRGDDVFIIAKVLLSKYAEELNSPVRGFSPQALS
                     AIRRYNWPGNIRQLENRIKKALVLCDKSLLGPEDLDLGEGAQAPIVPLEKAKEDFQRR
                     YVLDVLERNNGNRTQTARDLGVDPRTIFRYLEKEPGLAAGAGHGVDPEA"
     gene            complement(3782..4837)
                     /locus_tag="VFQ61_26315"
     CDS             complement(3782..4837)
                     /locus_tag="VFQ61_26315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958050.1"
                     /translation="MKLAWLRSVSAWCLALAGVAIPATAAAQVPPPGYGGSGTAYTGV
                     PNYGAPPQGYGYSTAGYGSSSRRRSVLELGALYATSVAYGAGVGIWIDAEARVKDPGV
                     ALIAPAVLGVAAPAAVYFADVSPMPRGVPAAIAAGAVIGAGEGIGIASLQFVTSDKED
                     AWGFRGLARATALGATLGGVGGAALGYLQRPSPRSSVLMTSSVAWGSAIGAMFGYGST
                     GAGVGYGRGNDRAAWGGLIGYNVGLVATAALSTVYIPTTLSLEWMWAGAGIGFAASLP
                     VYLFYIGDGGPPGKRGLLFSGTATTLGIVGGALLTAESRESAAAPRPSRQFARITNLL
                     PMLGAGNLGLSLAGEIE"
     gene            complement(4834..5880)
                     /locus_tag="VFQ61_26320"
     CDS             complement(4834..5880)
                     /locus_tag="VFQ61_26320"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET9958051.1"
                     /translation="MAHIAVLGAGAWGTALARHLVRQQPNASGQANPVPPSPHTVRLW
                     SWQAEHVEELLRDSENRRFFPGFDLPSAIEPTASLEHCVHGARAVLVVVPTQAVRSVL
                     EKARASLSPDSLLILASKGIENGTSMLLSEVVVDVLGAAFQKRTVALSGPSFAQEVAR
                     GLPTNLVAAALEARAAEAAQSLIASERLRVYTSDDPVSVEVGGALKNVIAVAAGASDG
                     LGFGHNTRAALITRGLAEMARLAVAKGGNPLSLAGLSGLGDLVLTTTASLSRNRTVGF
                     ELGRGRELEDVLRTLGHVAEGVTTARSAYDLAVRLKVDLPITTQVYRVLYEHKPVAEA
                     VEELLQRPAGREWS"
     gene            6084..>6480
                     /locus_tag="VFQ61_26325"
     CDS             6084..>6480
                     /locus_tag="VFQ61_26325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958052.1"
                     /translation="MSTPVSLKSGISAILLVALSACGSDGSPDIGTGGLVGNGGSLSP
                     SGGSTVTSGGSTAVSGGTTAVSNGGSSSGFGGSSLGGSTQGQGGSVNGGSSQGGTLGG
                     GSQGGAAGGVGGKSMGGFSNGGDGGTAGTG"
BASE COUNT         1238 a         2150 c         1973 g         1119 t
ORIGIN      
        1 gtcggagccc gagcattccg acccgcaagg ccaggttccg gattccgagg cgctcacggc
       61 ccgcactccc gcctacggct cgaaacccca agcggtcccc gaagcagcgc tcatcgaggc
      121 tctcggctgc gagccgtcgg ttccagagcc gccgaccgca tcaccaccgc tcgtcgcgct
      181 cgcgcagtcg gcgacctcca cccgcacatc ttccgcgaaa ggggtccacg gaactttccc
      241 cgagcgccaa aagaatccca agagccccac cgcacgtgct ttgggcgagg cgctcgccga
      301 gttagccgag caagcccggg aagagccggt cgcggttcgg accgaagctc ccaccgcgct
      361 cggcgacatc gccgacgagg tactcaggag cttgaccgtg caagacgaac cggttgccag
      421 caaccgctcc gaaaagcagg tcgaggagct ttcgagcttg gccgctctgg tcacggaagc
      481 accgcaacgg gacctgcccg agagcacggc gacgcgcgct cccgagcttc ccccgctcta
      541 tcccaatgcc cccagcgagt ggcgcaattg gctgcgtgac ttggaagcag cacgcggccc
      601 caagccctta tccgcaatcg agcggatgtt cattaccagc tacttgccgc tcagctatgc
      661 ctgcgtgcac ggctatgcgg atccggctgc cgaacgcgcg ctcgagaact ggtcgcagag
      721 cttcgcgcag agttactccg aggcgttcga tgctctgcga gtacgtggca agcggcccag
      781 catggtgctc gacgcgccgg agcttgcgct gcgaattgga cggcttcacg gcgctcgctc
      841 cgtgcagttg ctcctggtgg acggcatgcg cttcgacttg gggctgctcg tcgagcagcg
      901 cgtgcgtcta gcactaggac aggacgccgc gctgaccgaa cgcctcttgt tgtgggctgc
      961 attaccgacg acgagcgagg tgcaactgga tctcttggga cgtggtccag aagcgctcaa
     1021 agaaggacgg acttctggcg gctccgacgt gaccgtcgcg cggggacgcg ccgcggccac
     1081 tctgcgacga gtaaagacgc acaatcgtga gctgctcaag ctcgatctgg tcgaggcccg
     1141 cctcgcggag ccgggcccac gcgaagcgca acgcctctcg agtttggccg acgagaccgc
     1201 cgcgattttg gtggagcaca tttccaagca ggccccgcgc accttggtat tcgcgttcgg
     1261 ggatcacggc ttccgactcg atccactcga gaagggcacg cgtgccgcgc aaagcggggg
     1321 cgctacgccg gaagaagtgc tggtgccggc gtttgcttgg ttagttggcg gcgtccactg
     1381 agttagtggc ggcgttcacc aatcgccgcc tgcgtctatg cgcgaaccac gtcctcagga
     1441 tgttgttcac gaactcgcgg gtgctcaagc ttccggatcg acgccgtggc ctgcacccgc
     1501 cgcgagccct ggttccttct ctagataacg gaagatggtg cgcggatcga cgcccaaatc
     1561 ccgcgcggtc tgggtgcgat tgccgttgtt gcgctccaac acatcgagca cgtagcgacg
     1621 ctgaaaatct tccttggctt tttcgagggg cacgatcggc gcctgagccc cttcccccaa
     1681 atccaagtct tccggaccca gcaagctctt gtcgcacagc accagcgcct tcttgatccg
     1741 gttctcgagc tggcggatgt tacccggcca attgtagcgg cggattgcgg acagtgcctg
     1801 cggtgagaag ccgcgtacgg gactgttgag ctcctctgca tatttgctga gcagcacttt
     1861 ggcgatgatg aacacatcgt ccccccgatc gcgaagcggc ggtagccaca ggttcaccac
     1921 gttcaggcga tagtagagat cctcgcggaa ctcccccgtc ttgatcatct cgtcgaggtt
     1981 acgattggtg gcggacacga tccgaatgtc gcacttctcc ggtcggctat ccccgacgcg
     2041 aatgacgacg cgctcctgca gcgcacgcag taacttcacc tgcagctgcg ccggcaactc
     2101 gccaacttcg tccaaaaaca gcgtgccgcc atccgcttgc tggaacttac cggggcgact
     2161 ggcgatcgca ccggtaaacg caccttttac gtgaccgaac atctcgcttt cgatcaagtt
     2221 ctcgggaatg gcgccgcaat tcacggtcac gaacggcccg ttcgcgcgag aggagcgccg
     2281 gtgcaactca cgcgcaatca gctctttgcc cgtgccggtc tcccccgtaa tcagcacact
     2341 gatgtcggtg ctagcgacct tctgcagctt gcggaacacc tccatcatcg agggacacgc
     2401 gccaatgatc tcgccgaagc gcttgtcgga gagctccgcg ctgagcttct ccttgtcggc
     2461 gcgcagcgcg ctgagcagca tcgcgttctg caaaatcaac gaggcctggc tggcgtagac
     2521 ggtgagcagg tcgagctttc gacgatcgaa caaatgcttc acctcgtcat tggcgacgta
     2581 gagcgcgccc acgatctccc cttgcccgag caagggcacg cacatcacgc tcgagagctt
     2641 gagcgcgatg acgctctcac tcttgccgaa cgtcgtgtcg ttcagggcat cactgacgat
     2701 gacggcgcgc ttggtctcga tcaccttgcg cacgatgctg tcgctcatca gaccggagga
     2761 gtcttgaatc gcttcccgtt gcacgttgcg agatgcgcgc acgcgcggca gcacatcctt
     2821 gctgtcaccc ggatcgaccc agagtaccca gccgcgcgcg gcaccggtca gctcgatcac
     2881 gtcatcgagc agggtctcca atagctcgtc cacggagcga gtcgtcatca gacgttcgct
     2941 gaacgtctgg agacgccgca aaccagacaa gtcgctctgc atctcgctcg cgcccgtgtc
     3001 cgacacgggc tcccccgcat cttcgctgaa catgctgaag gcgagctgag ccttcccgag
     3061 cgtgatgcga tcgccgttgc acaagcgtgc gcgcctcttc ttcttaccgt tgatccaaat
     3121 ctcggcctct cggtcgacct cttccagctg gaagtcgcga ccgttgaaca cgatctggat
     3181 gtggtgggca gctacactgg catctgggat ctgaacgtcg ttgcctagag ctctgcccac
     3241 cgtggtgacg ggcttatgca acgcgaggag aacctggtta ccgtcgttcg agaagtaacg
     3301 gatggtcgcc atccggtcga aaagttacca gaaacaccgg cgacaagatc cccggctgtg
     3361 cggcccatca gacttttacg aaagtatgcg agttgcggag atcgccgccg gcggtgctcg
     3421 actcgcaagc accgccggcc ccgacacgct ccgcccaccc ccgcatcttg cccgtcccaa
     3481 cgggttctcc tttggggtgg ccccggaaac taggggacca agtagggaga aaacgaaaag
     3541 gaagccgaaa agctgctgcc ctggcgcgtg atgagccgag cagcgccgtt tgaaaccacc
     3601 gagcccgtag tgcccgaggg cgtcaacgac gacgccccga gggagcaagc gccgggccaa
     3661 ctcggttgac gcgaaacttc agtaaaaaat cgaggcgccg ccaaaactcc gtcgccgcgc
     3721 gtggctcagc ttggcacagc gcagtggctc gagtgtctta ctgaaagctc gccgtcgagc
     3781 ctcattcgat ttcgccggcc agactcaggc cgagatttcc ggcgccaagc atcgggagga
     3841 ggttggtaat gcgagcgaac tgtcggcttg gacggggtgc cgcagcgctt tcacgagact
     3901 ccgcggtgag caacgctccg cccacgattc ccaaagtggt cgcggtgccg ctgaatagca
     3961 aacctcgttt gccgggtggt cccccgtcgc cgatgtaaaa caaatacaca gggagcgacg
     4021 ccgcgaatcc aattcccgcg cctgcccaca tccactcgag tgacaacgta gtgggaatgt
     4081 agacggtgga gagcgcggca gtcgcgacca ggccgacgtt gtatccaatc agaccgcccc
     4141 aagctgcgcg gtcattgcct ctaccgtagc caacgcctgc gccggtcgag ccatacccga
     4201 acatggcccc gatcgcactg ccccacgcga ccgagctcgt catcaacacg ctgctgcggg
     4261 gtgacggtcg ctgcaggtag cccagagccg cgccgcccac tccacccaaa gtggcaccca
     4321 acgcggtcgc acgcgcgagc cctcgaaagc cccaggcgtc ttctttgtcg gaggtaacga
     4381 attgcaagct cgcgatgcca atcccctccc cggcgccaat cacggctccg gccgcaatcg
     4441 cggccggcac accgcgcggc atcggggaaa catcagcgaa gtagaccgcg gctggtgcgg
     4501 caacccccaa cacggcgggc gcgatcagag cgacgcctgg atccttcacc cgagcttcgg
     4561 cgtcgatcca gatgccaacg ccagcgccgt aggccaccga agtcgcgtac aacgcgccga
     4621 gctccagcac ggaacggcgt cgtgaactgg agccatatcc cgccgtcgaa tagccgtaac
     4681 cttgaggagg ggctccgtag ttcggaacgc ccgtatacgc agttccggac cctccgtatc
     4741 ccggaggcgg aacctgggcg gccgccgtgg ccggaatggc aactccggcg agcgctaagc
     4801 accaagcact gactgagcgt aaccaagcca acttcatgac cactcccgac cagcaggtcg
     4861 ctgcagcaac tcttcgaccg cttccgcgac cggcttatgc tcgtaaagca cgcggtatac
     4921 ctgggtcgtg atgggtaagt cgaccttcag gcgaacagcc aagtcatacg cactgcgagc
     4981 ggtggttact ccctcagcga cgtgcccgag cgtgcgcaag acatcctcga gctctcgccc
     5041 gcgcccgagc tcgaagccca cagtccggtt tcgcgagagg ctggcggtgg tggttagcac
     5101 gaggtctccg aggccggaca ggccggccaa actcaaagga ttgcctccct tggccacggc
     5161 gaggcgagcc atttccgcga gcccgcgagt aatgagggcc gcccgtgtat tgtggccaaa
     5221 cccaagccca tccgaggctc ccgccgccac ggcgatcaca ttcttcagcg ctcctcccac
     5281 ctcgaccgag acgggatcgt cgctggtgta aacccgcaaa cgctcactcg cgatcaggct
     5341 ctgcgctgcc tccgccgctc gagcctccag cgccgccgca accaggttgg tcgggagccc
     5401 gcgcgcgact tcttgtgcaa aactcggccc ggatagagcc accgtacgct tctggaacgc
     5461 ggctcccaag acgtccacga cgacctcgga aagcagcatc gaggtcccgt tctcgatgcc
     5521 tttgctggcc agaatcagga gcgaatcggg cgacagcgac gctcgtgcct tttccagcac
     5581 gctgcgtacg gcctgagtag gaaccacgac cagcaccgct cgtgcgccgt gaacgcaatg
     5641 ctcgagtgag gcagtgggct cgatcgcact tgggaggtcg aagcctggga agaaacggcg
     5701 attttccgag tcgcgaagca gctcctcgac gtgctccgct tgccaggacc agaggcgcac
     5761 ggtgtgtggg ctcgggggca ctgggttggc ttgcccagag gcgttgggct gctgccttac
     5821 gagatggcga gctaacgcgg tcccccaagc acctgcaccg agaacagcga tgtgagccat
     5881 ggcgggcgga ctcttggggg gattcggccc acctgtcaac cgacgcggtt agcctggtct
     5941 gaccaacaac ctatttctac agatgccgta aagcttgcct gctgcggcat gcgcgcagga
     6001 agtctcgtct ctctgaaatt aagctgcctg acatcagccc taggacggca ctccaacacc
     6061 actcaaaccc caaaggactc tacatgagta cacctgtgtc actaaagagt ggaatctctg
     6121 caattctcct agtcgccctt agtgcctgcg gctcggacgg cagtccggac atcggaaccg
     6181 gcgggctcgt tggcaatggc gggtcgctct cacccagcgg tggaagcact gttacgagcg
     6241 gaggctcgac cgcggtttcc ggcggaacga ccgcggtctc taatggcggc tcgagcagcg
     6301 gcttcggagg aagctcactc ggcggatcga ctcagggtca aggcggcagc gtcaatggcg
     6361 gctcgagcca aggcggcact ttgggcggtg gaagccaggg cggagccgcg ggtggcgtcg
     6421 gtggcaagtc catgggcggc ttctccaacg gcggcgacgg cggaaccgct ggaacgggcg
//
LOCUS       DASTKU010000754         5681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_816996, whole genome shotgun sequence.
ACCESSION   DASTKU010000754 DASTKU010000000
VERSION     DASTKU010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816996"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(583..1026)
                     /locus_tag="VFQ61_26330"
     CDS             complement(583..1026)
                     /locus_tag="VFQ61_26330"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET9958053.1"
                     /translation="MHQDFQDLLTVFAEEQVEYLVIGGYAVSYYARPRYTKDIDLWLR
                     DDRANLERAVMALQRFGVPEPVLAGLQTESAEDIVWFGVAPTRVDLLRRVDGLSFDSA
                     YARRVETDWGDVRVCVVALPDLIAAKRAAGRPQDLRDLKALERIK"
     gene            complement(974..1210)
                     /locus_tag="VFQ61_26335"
     CDS             complement(974..1210)
                     /locus_tag="VFQ61_26335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958054.1"
                     /translation="MDSGFEQRAAERRTRVVGGTARSHDELDELDFEFWQSCSGGERL
                     EATWQLALDAITMKAQPNAPGFSGPAYGVRRRAG"
     gene            2278..2487
                     /locus_tag="VFQ61_26340"
     CDS             2278..2487
                     /locus_tag="VFQ61_26340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958055.1"
                     /translation="MAFLRCELCGHNRAPALRAAGGEGRAPRGPREESVAATTSSTRA
                     PRDIRGLFPIQDAIPEFANLANLRR"
     gene            complement(2643..2725)
                     /locus_tag="VFQ61_26345"
     tRNA            complement(2643..2725)
                     /locus_tag="VFQ61_26345"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2690..2692),aa:Leu,seq:taa)
     gene            2904..3623
                     /locus_tag="VFQ61_26350"
     CDS             2904..3623
                     /locus_tag="VFQ61_26350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958056.1"
                     /translation="MPLRLPGVALVALCTSAGCTSQVEPIAVGDECPARPLRGPLAAV
                     PTEEVDELLIDDFERGDNQLEHISGRDGAWVVGSDATSVVLTAESSSHCAARGREAGH
                     FAGRDFTEWGANWTAVLKQFVNQMARPYDASAYGGISFWAAVGAGWKGELPFTTPVGV
                     TTMDVAFNGGMCTQCMDYYRTNVRLSSSWERYEIRFDDLHQLGIGDPLVPLRKDQLVG
                     LIFWPPNSGFDIWVDDVRFER"
     gene            complement(3669..4802)
                     /locus_tag="VFQ61_26355"
     CDS             complement(3669..4802)
                     /locus_tag="VFQ61_26355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958057.1"
                     /translation="MTFAHRLLVLLLPLAALPCARQGLAAPADGRSTDSDTTSRSAPT
                     PNSSAPGTPAGSGLVLLIAERPDDPFLERIKAELVAIGFTTVTRRNDVPLERAAREVS
                     AVAAIRVLPSRQGVEVWMADRTSGRSLLRQVIVDENPSGPNQSVIALQTAELLRTSLL
                     EQQAAPPASSAGHGPPPTPPTPPEASFPPTKDEPATPVSAAGTPPPSAGLQAALGALY
                     SPGGSTPALQLWLSLQRSFSQNVALAIDLSAPLLNASRTLPEGTTRIGTYVAGIAFLL
                     QTRSQSGRFFANGGAGLGAMRLAVAGETSAPLVSRSVGVTSVAGYIRSDVGAELAPWL
                     RLGARAIAGATPEKLEITVAGNEGGSWGRFYAGAFFLAEVPWR"
     gene            complement(4799..>5681)
                     /locus_tag="VFQ61_26360"
     CDS             complement(4799..>5681)
                     /locus_tag="VFQ61_26360"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9958058.1"
                     /translation="SDANGARVLLEAGEAEVSIQHRVGRKARWNVEAGPYRVAVTGTR
                     FRVAWNPAEQAFELALHEGNVLVSSNRCLQQERVVHAGETLALSCATRSKVAASASVE
                     VVPTAPPVAGAPSGATWHAQAPKASARAVTTAETEALDAFREPLAAGHFAEALRLAER
                     AHFERVIANASRSDLLQLADAARLSGRIDRAVQALGAVRQRFPGSEEAGTAAFSLGRI
                     AFDQHKAYGEASQWFSTYLAEQPGGPLMGDAFGRLIEARERSGDLAGARTEAERYLRR
                     FPQGPYAAEARRILATE"
BASE COUNT          984 a         1772 c         1901 g         1024 t
ORIGIN      
        1 cgctgatcgc gatatttgtc tcgagcttta cggacgtcat tcttgctcgc gctatcggtc
       61 tattagcgtc ttcgtcaccg gtggcttctg ctcttggcgt tgcgcaacac tgaaccgttc
      121 caggcttcgg ggcgggcttg ctcacaaccg attgctcgct cgctacggcg cgacggatca
      181 gcagagcccg tgcggtcctc gaagcttgag ccgaggcatc actcaggcgg ctggtcgtcg
      241 ccgccgagcc gctgcactga acacccaact gtccccgcgt gctctcgatg ctcgtccctt
      301 gccgcgaaac gtaacgagca tcttttctgc tgagtagccc ctcccgggct cgaaccggga
      361 ctccatcgct ggaaagggat tttaaagcgg gagcgaattc gggaattggc aggatttgca
      421 cgggatttgg tgcgcgtggc tgctggaaag gcgtcgtatc gcgttcgcta agcgcgcgag
      481 ggacagttgg cgggacagtt tgagcctggc gcgttgtccg cagcgtggac accgtccgcc
      541 cgcgctgata agcggagagc tcgaggccgg ccgaagaagc ggctatttga tgcgctcgag
      601 tgccttcaag tcccgaaggt cttgcggcct cccagcggcg cgtttagcgg cgatcaaatc
      661 tgggagggca acgacacaga cgcggacgtc tccccagtcg gtctcgacac ggcgagcgta
      721 ggcgctgtcg aagctcagcc cgtcaactcg ccgcaggagg tcgacgcgcg tcggtgccac
      781 gccgaaccaa acgatgtctt cggcgctttc agtctggagg cccgcgagca ccggttcagg
      841 aacgccgaaa cgttggagag ccatgacggc gcgctccaga ttcgctcggt cgtcgcgcag
      901 ccacaggtcg atgtctttcg tgtatctcgg gcgagcgtaa tagctgacgg cgtagccgcc
      961 gatgaccaag tactcaacct gctcttcggc gaacaccgta agcaggtcct gaaaatcctg
     1021 gtgcattcgg ttgcgctttc atcgtgatgg cgtcgagcgc cagctgccaa gtggcttcga
     1081 gtcgctctcc cccagagcaa ctttgccaaa actcgaagtc gagttcgtcc agttcgtcgt
     1141 ggctacgagc cgtgcctccg acgacccgag tccggcgctc tgcggcgcgc tgttcgaagc
     1201 cagaatccat ggtccggtag tatagccgat cgggggccac tcgcgccgct ggtcaggttt
     1261 ggggcggcaa cacccgggac gaccggtgcc gttctcctga aatgccgggc atcccggcgc
     1321 gcggtgcagg cgtcgtacgc tctgtcggct gtgggcttgg gtgattcatt ggtaattgcg
     1381 gaactgcagg cgagccctca cccagtttcg cacgacgaag tactccatcg cgaaaggatc
     1441 gtcggcgttt gcaagcgggc ccgcggcgag gtgcgccacg cagccagcga gaaacggcga
     1501 ccccaccacg ttgaagtcct ggtttggcga agtccggaga gcggacccgt cggactgcac
     1561 gagacggcgg catcgagaac gccgacccgt acttcgccct gctcgaagac gtcgtcatcc
     1621 gtatccggca cggtccctcc aattgaaagc aagcccgacg ctggttgaca cgttgcgccg
     1681 ttgcaactcc gccaaagacg attggctgac taccggtaaa ggctcaccgc tcaccgagct
     1741 cgctctggat ggacagatgg cgcccgatgg tcaacgacgg cgggggctag gcggaacttg
     1801 ctcgccgagg tcctgcagtc ttcgctcgat ttcagccttg cgggctcgga attcgacgcg
     1861 agcagcagct gctttgagct ctctgtcatt cttcgcgaga agaacctcga ggccttgaat
     1921 gggtatcacg atcgcttccg cgctcttccg aagtccaagc acccttgctc gaagggctgc
     1981 gagcatacct ataagtcctg aaatttctgc gaatatcgtg taaccgagcg gccgaatgag
     2041 caccgagatg agggcaagcc cccatgcggc caagtgaatc ctgcggtcct tcaggatggc
     2101 actttcacgc gcgcgcaatg cgctattacg tagtagtcga attcgctctc acatcgaagc
     2161 accctccgtg tgttgttgag cgaagcactg tagctcgagc gcgcggaagg tcgcccgagt
     2221 tttcccaaag ttttcccaaa gtgacacgtg agagtgcgtc tcccaggagg agacgccttg
     2281 gcgttcttgc gttgcgagct ctgcggccac aatcgagcgc ctgcgctgcg cgctgccggc
     2341 ggcgaaggtc gcgcgccgcg aggtccacga gaggagtccg tcgcggcaac cacctcgagt
     2401 acacgagctc cgcgcgacat tcgcggtttg ttcccgatcc aagacgcaat ccccgagttc
     2461 gcaaatctag ccaacctgcg acgttgaaca cggcggggtg gacgcaactg cgcgtcgctc
     2521 tcggtacggc aacggcgcgc tgcgttgtgg gcagggcaca cgcaaagcac tgaacaccca
     2581 actgtcccgc gtgctctcga tctcgttctc gccgcgagct gaacgaggtc ttttctactg
     2641 agtagcccct cccgggctcg aaccgggact ccatcgctgg aaagggattt taaatccccc
     2701 gcgtctgcca ttccgccaag gggccgtgtg gtgggtgcgg agcgggagat agcacacggc
     2761 tccgcgccgg tggaggcaaa ggattctgcc ctcttcgagc ggcgcgccgg cccatgccgc
     2821 gcgaagagta gatcagcaga aaagctgcgg gtcggtgctt gccgcttgag ccgtcgcggg
     2881 tcgcggtgga aactgcaggg tcgatgccac ttcgcctgcc aggtgtcgcg ctcgttgcgc
     2941 tgtgtacgag cgctgggtgc acgtcgcagg tggaaccgat tgcggtaggc gatgagtgcc
     3001 cggcgcggcc gctgcgtggg cctttggccg cggtgccgac cgaggaggtg gacgagctgc
     3061 tcatcgatga tttcgagcgg ggcgacaatc agctggagca tattagtggt cgcgacggcg
     3121 cttgggtggt cggatcggat gcgacgagcg tggtgctgac ggccgaatcg tcttcgcact
     3181 gcgccgcacg cgggcgtgag gcagggcact ttgccggccg agatttcacg gaatggggcg
     3241 cgaattggac ggcggtgctg aagcagttcg tgaaccagat ggcgcggccc tacgacgcct
     3301 ctgcatatgg ggggatctcg ttttgggcgg cggtgggggc cggttggaaa ggggagctac
     3361 cgttcacgac tccagttgga gtcaccacga tggatgtggc cttcaacgga gggatgtgca
     3421 cgcagtgcat ggactactac cgaaccaacg tgaggctgtc ctcgagctgg gagcgctacg
     3481 agatccgttt cgatgacttg caccagctcg gcattggcga tcccttggtg cccttgcgca
     3541 aagatcagct cgttgggctg attttctggc cgccgaacag cggattcgac atttgggtcg
     3601 acgatgtgcg attcgagcgc tgagtcgtcg aaggacaatc cgcgcggctc ggacacccga
     3661 gggtgagctc agcgccaggg cacttcggcg agaaagaatg cgccggcata gaagcgtccc
     3721 cacgagccgc cttcgttccc agccacggtg atttcgagtt tttcgggcgt tgcgccagcg
     3781 atggcccgcg cgcccaagcg gagccacggg gcgagttcgg caccgacgtc gctgcggatg
     3841 tagcccgcaa cgctcgtgac cccgacggat cggctgacga gtggggcact ggtttccccg
     3901 gccactgcca gtcgcatggc tcccagcccg gcgccgccgt tggcgaaaaa gcgtccgctc
     3961 tgactgcgtg tctggagcaa aaatgcaatt cccgccacgt aggtgccgat gcgcgtggtg
     4021 ccctccggca aggttcgcga agcgttgagc aacggagcgc tcaaatcaat cgcgagcgcc
     4081 acgttttgcg agaaggagcg ttgaagcgag agccacagct gaagcgctgg agtggagccg
     4141 ccggggctgt agagcgcgcc gagcgcggct tggagaccgg cgctcggcgg cggcgttccg
     4201 gctgcggaca ctggggtcgc cggctcgtcc ttcgtcggtg ggaatgacgc ttccggcggt
     4261 gtcggcggtg tcggcggagg gccgtgccca gcgctcgacg caggcggcgc agcctgttgc
     4321 tccaagaggc tcgttcgcaa gagctccgcc gtttgcaggg cgatgacact ctggttgggg
     4381 ccggaagggt tctcgtcaac gatgacttgc cggagtagag agcggcccga ggtgcgatcg
     4441 gccatccaca cctctactcc ctggcgagag ggcagcacgc ggatggccgc gacggcgctc
     4501 acctcacgcg cggcccgttc gaggggaacg tcattgcggc gagttaccgt cgtgaagccg
     4561 atggcgacga gttcagcctt gatgcgctcg agaaaggggt cgtctgggcg ctccgcgatg
     4621 agcagcacga ggccgctacc ggcgggcgtg ccgggcgcag acgagttcgg ggtcggagcc
     4681 gaccgagacg tcgtatccga atcggtagat cgcccgtcag cgggcgcggc tagcccctgg
     4741 cgcgcgcatg gcaacgcggc gagcggcaaa agcaggacaa gcaggcgatg ggcgaaggtc
     4801 actcggtcgc aaggattcgt cgagcctccg cagcataggg gccttgcggg aaacggcgca
     4861 agtagcgctc cgcttcggtt cgggcacccg ccaaatcgcc cgaccgttcg cgcgcttcga
     4921 tgagacggcc gaaggcgtca cccatcaacg ggccgcctgg ctgctcggcg agataggtcg
     4981 aaaaccactg gctcgcctcc ccataggcct tgtgctggtc gaatgcgatg cgacccagcg
     5041 agaaggccgc tgtccccgcc tcttcggagc cgggaaagcg ttgccgaacg gcgccgagcg
     5101 cctgcaccgc gcgatcgatg cgccccgaaa gacgcgcggc atcggcgagc tggagtaggt
     5161 cgctccgact cgcgttcgcg atcactcgtt cgaagtgcgc gcgctccgcg agccgcagag
     5221 cctctgcaaa gtgccccgcc gcgagcggct ctctgaacgc atcgagcgcc tctgtttctg
     5281 cagtggtcac ggcgcgcgca ctcgctttgg gcgcttgcgc gtgccaggta gctccgctcg
     5341 gcgcgccagc taccggagga gcagtgggaa cgacctccac ggacgccgac gcggcgacct
     5401 tggaccgggt ggcacagctc aacgccaacg tctcgcccgc gtgcaccact cgctcctgct
     5461 gcaggcagcg gttgctcgag acgaggacgt ttccctcgtg cagagcgagc tcgaacgctt
     5521 gctcggcagg attccaagcg actcggaacc gcgtgcccgt gaccgccacg cgatacggtc
     5581 ccgcttctac gttccagcgc gctttccggc ccacgcggtg ctgaatcgac acttcggcct
     5641 cgcccgcctc gagcagcacg cgggccccgt tcgcgtcgga g
//
LOCUS       DASTKU010000755         5290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1935549, whole genome shotgun sequence.
ACCESSION   DASTKU010000755 DASTKU010000000
VERSION     DASTKU010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1935549"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="VFQ61_26365"
     CDS             <1..1190
                     /locus_tag="VFQ61_26365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958059.1"
                     /translation="VVQDGVMRFKLAKNLCESNYRKKKILALEATAACPSKRLFQPLC
                     TDYTAPDPRTRDDAPQTGTCTIANLDAVESAVYVLLDRSRSMNKFYEGNDMALTLKST
                     VGLPLSTPVAERTKVGFEFLPPTAAQCSNLDYENPRVAIGEVTQARDAIVGLLDGDAV
                     LADNPSSFFLQAALQGAYASVGAVRASGQRFNRRAVVVVSNRDILNGQCSSVPAIELA
                     RAARNRADPVFTYAVALDDGEDAALSSATELAQAGGTRVIDGVSNEEDGARAVQDILT
                     ELGTCLYDVKRADAGGSARLPKTAKVAYIDVDRSTDSGTGESSRGIEIEYAEGCVDAS
                     SSVSGWNQGEDGLLRLCGQACEDLRRVVGNSAVTQLVQGRPAPAVPLVVTAPCSEYEV
                     GRE"
     gene            complement(1230..2441)
                     /locus_tag="VFQ61_26370"
     CDS             complement(1230..2441)
                     /locus_tag="VFQ61_26370"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9958060.1"
                     /translation="MPTPLAASASGRSAALKRWLGGSLGLGLSLWALPLAAQAGVAPA
                     PSPQTAPAPARPGAAGSATAPSTTAAPGSVTTAPSANAEGELSVSIADGQGTISLDGL
                     EVGQGSFRGKVRVGKHQLRVTSDGYQPYEALVDVAANELRSESVALRPSVGWASYQSS
                     GPIRDEHGLYGGVALLGALEPTGSGTTLEDSCDTTGATSCSAGTVLAGGLTGYLGWLF
                     DPLGLEVALLASADVVRPSAKFDGENGSDINPIVARPAREEKFTIGRFGGGVALRGRV
                     SYALPGVRLSAAAGPGLSYRYLAFRRKTEADGGLRGETAESGADYFSPLLSVELGAQV
                     RLTRSLALAAGVASWFEHAGDSTVSTPKNNTYLTGDSDSLPATQATPAYHLANGFQWF
                     IGPFLGVTFGP"
     gene            complement(2626..5214)
                     /locus_tag="VFQ61_26375"
     CDS             complement(2626..5214)
                     /locus_tag="VFQ61_26375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958061.1"
                     /translation="MELRFITPRYYTRLGTAAFIALGCSSGSATNSPVNGGATNSGGA
                     APSSSAGTPSGAGTSSSGGGTWSAPAGAGTANARGGGAGTSTAAGAPASSGGRGGASS
                     VGGAAGGPPSNPDDTSGWGNVRFDGGGFVDGIIASRQVKGLFYARTDVGGAYRWSSEK
                     KVWIPLLDWLPQEDSGLYGVESIALDPSNPERLYVLAGTSYFSNGKTAILRSSDYGAT
                     FETVDVSSLWRAHGNGMGRQSGEKLAVDPHNPDILFCGSRTAGLFKSSDAGKSWDKVS
                     TLGAQSSADLTNTNGISFVLFDPASSATASGATSTLYLGVSATTNNLYVSKDGGATFE
                     PVAGGPAGQMPNRAVLDRGNLFITYSGSLGPHSVSTGSFYRYTVASNTWTNLTPKSDD
                     GTAYQGSGGQQYAHAFGGISVDPDDPNHLLLSTLGYYGGQTRYADGGEGWGDRIYVSH
                     DGGAKWTTSFSYLDPKIPANANAATNGNGWISGNAIHWAGDITFDPANPKEAWVVSGN
                     GVFHTLDLDAEKPIWNFESRGIEETVPLDIVSVPGGPLVTAIGDYDGAVYQDITSTYP
                     RHNPAMGTTHSLGYAPLAHAFLRAGHVTDYSTGTAVESDVMYYSADDAATWSKLPTPK
                     GSHGLVVLSADGNVLLHRPDNAASVYRSADQGQTWTPVTGLDGQAQYARIVCDPVNAD
                     VFYVLNQQGKLLKSTDKGASFAVVGSIQDDAKSLYQSSNGLIRTVPGREGHLWAPLDQ
                     PQSWASNGKYSTNGLAASEDGGVTWSRFPTVFSAQAVGIGKAAPNATYETLFIYGVAG
                     ESTNPVGIYYSTDKGASWKRMNDDRHQYGGPGNGAFVQGDMNVFGRVYMSTVGRGLVY
                     GHVAAL"
BASE COUNT          907 a         1685 c         1765 g          933 t
ORIGIN      
        1 cagtcgtgca agatggcgta atgcgcttca aactcgccaa gaatctgtgt gagtcgaatt
       61 atcgaaagaa gaagattctc gccctcgaag cgacggcggc ctgtcccagt aagcggttgt
      121 ttcagccgtt gtgcaccgac tacacggcgc ccgatccgcg cacgcgggac gacgctcccc
      181 agaccggcac ttgcacgatt gccaatctgg acgcggtgga gagcgccgtg tacgtcctgc
      241 tcgaccgttc gcgcagcatg aacaagttct acgaaggcaa cgacatggct ctgaccctga
      301 agagcacggt gggattgccg ctctcgacgc ccgtcgccga acgaaccaag gtcggattcg
      361 agttcttgcc gccgaccgcc gcgcagtgct cgaatctcga ttacgagaat ccacgagtgg
      421 cgattggcga agtaacccaa gcgcgggacg ctatcgtcgg gttgttggat ggcgacgcgg
      481 tgttggcgga taatccgtct tcgttcttct tgcaggccgc gctccaaggt gcctacgcaa
      541 gcgtgggggc ggtgcgtgct tcggggcagc gcttcaatcg gcgcgccgta gtcgtcgtga
      601 gtaatcggga catcctgaat gggcagtgca gcagcgtgcc cgcaatagaa ttggcccgcg
      661 cggctagaaa ccgcgccgat ccggtgttca cgtatgcggt cgcactcgat gacggcgaag
      721 acgcggcatt gtcgagcgcc acggagctag cgcaagccgg tggaacgcgg gtcattgacg
      781 gcgtgagcaa cgaagaagac ggcgcacggg ccgtgcaaga catcctcacg gagctcggca
      841 cgtgcttgta cgatgtgaag cgcgcggacg ccggtggatc ggcgcgtctg cccaagaccg
      901 ccaaagtggc ctatatcgat gtcgatcgca gtaccgactc tggaacgggc gaatcgagcc
      961 gcggcatcga gattgaatat gcggaaggct gtgtagacgc gtccagctcg gtatcgggct
     1021 ggaaccaagg cgaagacggc ttgttgcgct tgtgcgggca agcgtgtgag gatctacggc
     1081 gtgtcgtggg aaactctgcc gtgacccagc tcgttcaagg ccggcccgcc cccgccgtgc
     1141 cgctcgtcgt gactgccccc tgctcggagt acgaagtcgg aagggaatga ggctgattcg
     1201 ggtctagacc cacggctcga atcgcgaggt cagggcccga acgttacccc aaggaacggc
     1261 ccaatgaacc attggaagcc attcgcgagg tggtaggcag gcgtcgcctg cgtggctggc
     1321 agagagtctg aatcacccgt caggtaggtg ttgttcttgg gcgtgctgac ggtgctatcc
     1381 ccggcgtgct cgaaccaaga tgcgacccct gccgcgagcg ccaagctgcg cgtgagccgg
     1441 acttgagccc cgagctccac cgacaacagc ggtgagaagt agtccgctcc tgactcggcg
     1501 gtttctcccc gcaatccgcc gtcggcttcg gtcttgcgtc ggaacgccag atatcgatag
     1561 ctgagcccgg ggccggcggc ggcactcaat cgcacgcccg gcagcgcgta cgagacgcga
     1621 ccgcgcagcg ccacacctcc gccgaagcgg ccgatcgtaa acttctcttc gcgcgccggc
     1681 cgggccacga tgggattgat atcggagcca ttttcgccat cgaacttcgc gctcggccgc
     1741 actacgtccg ccgaggcgag cagtgccacc tcgagaccga gcggatcgaa caaccaaccc
     1801 aggtaaccgg taagcccacc ggcgagcacc gtccctgccg agcagctggt cgctccggtc
     1861 gtgtcacaag agtcctcgag cgttgtcccc gagccggttg gctccagcgc tccaagcaac
     1921 gcaaccccgc cgtacaagcc gtgttcatcg cggatggggc cgctactttg ataactcgcc
     1981 cagccaacac tgggccggag cgccaccgac tccgaccgta attcgtttgc ggccacatcc
     2041 acgagcgcct catacggttg gtagccatcg ctcgtgacgc gcagctgatg cttgcccacg
     2101 cgcaccttgc cgcggaagga gccttggccc acctcgagcc catcgagcga aatggtgccc
     2161 tgaccgtctg caatcgagac actcagctcg ccctcagcgt tcgccgaggg tgcggtcgtc
     2221 acgctgccgg gggcagctgt ggtcgaagga gcggtagccg agccggccgc gccgggccgg
     2281 gcgggagcgg gcgcagtttg gggagacggc gcgggcgcca cgcccgcttg tgcagccaac
     2341 ggaagggccc agagggacaa tccgaggccc aaagacccgc cgagccagcg cttgagtgcg
     2401 gcggagcgac cagatgcaga cgcagcgagg ggagtaggca tgggtgaggc gccgagccgg
     2461 cgagcatact ggatccgcct gtcccagggg aaggcgcccg ccatccagtg gggcttcgcg
     2521 cccgaggccc tgtggtcgag gccgctcgag ggctacgcgc cccgcgcagc ttagggctca
     2581 tcggcacgag gatcgagctt cgctcgccta cgctcgctcg atggcctaga gcgcagcaac
     2641 gtgtccgtac acgaggcctc gcccgacggt gctcatgtag actcgaccga atacgttcat
     2701 gtcgccttgt acaaaggcgc cgttgcccgg gccgccgtac tggtgtctat cgtcgttcat
     2761 gcgcttccag gaagcgccct tgtcggtcga gtaatagatc cccaccgggt tcgtgctctc
     2821 gccggccacg ccatagatga agagcgtctc ataggtggcg ttgggggcgg ccttgccgat
     2881 gccgacggcc tgcgcggaga acacggtcgg gaagcgagac cacgtcaccc cgccatcttc
     2941 ggaggccgcg agcccgttgg tgctgtactt gccgttggag gcccagcttt gcggctggtc
     3001 gagcggggcc cagaggtgac cctcgcggcc gggcacggta cgaatcaggc cgttgctcga
     3061 ctgatagagg ctcttggcgt cgtcttggat cgagcccacg acggcaaacg aagcgccctt
     3121 gtccgtggac ttcaggagct tgccctgctg attcaggacg taaaagacat cggcgttcac
     3181 tgggtcgcag acgatgcggg cgtattgggc ttggccgtcc aaccccgtga cgggagtcca
     3241 cgtttggcct tggtcagcgg agcgataaac gctggccgcg ttgtccgggc ggtgcaggag
     3301 cacgttgccg tccgccgaga gcacgacgag cccgtggcta cccttgggag tgggaagttt
     3361 gctccaggtg gccgcgtcgt cggccgagta atacatcacg tcgctctcga cggccgtccc
     3421 cgtggaatag tcggtgacgt ggcccgcgcg caggaaagcg tgcgcgagcg gggcgtaacc
     3481 cagggagtgc gtagtcccca tggcggggtt gtgacgcggg taagtgctgg tgatgtcctg
     3541 atagacggct ccatcgtagt ctccgatcgc cgtcaccagc ggcccgccgg ggacactgac
     3601 gatgtccagg ggcacggtct cttcgattcc tctggactcg aagttccata tcggcttctc
     3661 ggcgtcgagg tcgagagtat gaaagacccc gttgcccgac acgacccaag cctctttggg
     3721 attcgccgga tcgaacgtga tgtcgcccgc ccagtgaatg gcattgccgc tgatccagcc
     3781 gttcccgttc gtggccgcgt tggcgttggc ggggatcttg ggatccagat acgagaagct
     3841 ggttgtccac ttggcgccgc cgtcgtggct aacataaatg cgatctcccc agccttcgcc
     3901 gccgtccgcg tagcgggttt ggccaccgta gtagccgagc gtagagagca gcaggtgatt
     3961 gggatcgtcc gggtcgacgc tgatgcctcc aaaggcgtgc gcgtactgtt ggccgccgct
     4021 cccttggtac gccgtgccgt cgtctgactt gggggtgagg ttcgtccacg tgttcgacgc
     4081 cacggtgtag cgatagaacg atcccgtcga cacgctgtgt ggcccgagcg aaccagaata
     4141 ggtgatgaac agattgcctc gatcgagcac cgctcgattc ggcatctgcc cggccgggcc
     4201 gcccgcgacg ggttcgaacg tggcgccgcc gtctttgctc acgtagaggt tgttcgtcgt
     4261 cgccgagacg cccagataga gagtgctagt cgcgccggag gccgtcgcgc tcgaagccgg
     4321 gtcgaacagc acgaagctga tgccattggt gttcgtcaga tccgcgctgg attgggcgcc
     4381 gagggtgctc accttgtccc aggacttccc cgcgtcggag ctcttgaata gccccgcggt
     4441 tcggcttcca caaaacagga tatcgggatt gtgcgggtct acggccagct tctcgccgct
     4501 ctggcgcccc atcccgttgc catgcgcccg ccacagcgaa gagacatcga cggtctcgaa
     4561 cgtcgcgccg tagtcactcg agcgcaggat ggctgtcttc ccgttgctga agtagctcgt
     4621 tccggcgagc acatacagcc gctccggatt actcggatcg agggcgatgc tctcgacgcc
     4681 atagaggccc gagtcttctt gcggcagcca gtcgagcagc gggatccaga ccttcttttc
     4741 gctactccag cggtaagcgc cgccgacgtc cgtgcgggcg tagaagaggc ccttcacctg
     4801 gcgagacgcg atgatgccgt cgacgaagcc gccgccgtcg aagcgcacgt tgccccaacc
     4861 actcgtgtcg tcagggttac tgggcgggcc gcccgccgca cccccgacgc tagacgcacc
     4921 gccacgcccg ccggaagaag cgggagctcc ggcggcggtc gaggttcccg cgcctcctcc
     4981 gcgagcgttc gccgtgcccg cgcccgcggg cgcgctccag gtgcccccgc cgctgctcga
     5041 cgttcccgcg ccgcttggcg ttcctgcgct gctcgacggc gcggcgccgc ccgagttcgt
     5101 cgcgccgccg ttgaccgggg agttcgtcgc agagccggaa gagcatccga gcgcgatgaa
     5161 ggcggccgtg ccgagtcgtg tgtaatagcg aggggtgatg aatctgagtt ccattccggg
     5221 gctttcctag ttcctcctgg accgccgggg cagcgcagga ggtcttcccg tagagtcgag
     5281 tgtgggtgcc
//
LOCUS       DASTKU010000756         4964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1488952, whole genome shotgun sequence.
ACCESSION   DASTKU010000756 DASTKU010000000
VERSION     DASTKU010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488952"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..734)
                     /locus_tag="VFQ61_26380"
     CDS             complement(150..734)
                     /locus_tag="VFQ61_26380"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HET9958062.1"
                     /translation="MSALAFALCLPCCAGGHAATNGSGALSGDVHDVAGGAARGPEAE
                     LACVSTAPERCFDARDNNCNGIIDEGCGVETGAVQFVIAWDAARADVDLQVIDPAGEL
                     VEVGRPVASGLVKSRDCPGRNNECRGRNFENVYLEAATAPRGRYLVRVVLEQLGGEPP
                     PVRVDFGARVGPRTYGREVLLSRVDVPFEAEFSL"
     gene            complement(778..3441)
                     /locus_tag="VFQ61_26385"
     CDS             complement(778..3441)
                     /locus_tag="VFQ61_26385"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET9958063.1"
                     /translation="MLEPNLPNLPPPSASRPPPSQVYLAKWPPDDDGPAVPPAPGGGF
                     PGSFDMGDGNFKKGRFNPVVILIGVLAVAGLAAFLLIGFKQDAEKLTVEQAEDRKKAI
                     FVLPKNEQIPQWRQWAGSEASDELRSEALKQLAWAKDPEGVALAVKALSYPSEGVQSM
                     AATSLAEYGRPLGEPARDALLAALKTAGKGAKPQIAWALVVLGDTRAFDEIMQLYRLG
                     HLSTVQRLGGGVAFDAAKIVDLVSLDKLASMANDESPSVRQLVATVLSDKAEPKWTDV
                     LIKLVQDKDPDVARQAAPGLGRIAESRAREPLIDAMKTADKDSRKKFLEALRDGIGTE
                     GLVLGLRSVSDDEKLAWYQTKQVFDMIHDTKNGGQGLNDPRGGNALYSYIESKPHTHY
                     QWRAAVALAAIGDVRAVPTLAKRLRMDPLKIYSDQRDWEMELKRDDNERVIASRMIAD
                     LAMLHPEKRQQIAEQAEDAVIFWIHELPSPHANGLRALAAMESTKDMDALRKWANPNV
                     PLPKEGQQPPMPEEFVVAQSALRYVGWMKDQRSWGVLEKSLNRRKNERDEYDVTMEGL
                     MQGGVAILGMSLRALGVGAAQGFSEWRDHRAFKWLLDYVADVKNNEQSRSEACAALAW
                     VAEKDDFLEVAKRIQEYGGSEKSDQFRRACLLETLIQRPVPGTAPALMSLMTPESSLS
                     TRHQVARAIAKGGFDQSVEAKLFEMMNSDVLVNDAALALILGGTPETAARAVALYANK
                     PKATLDELGELWYQSFGYWSTEDLESGLLFKYVDNAEAITRLSIRMTPQEWARVMLIR
                     QLDNLVFDNGPHSFTRVVLRHRLWQMAKGNDEAKRQGAIRTLKFMKEQGPLLALRDEQ
                     GQTGQLARAAYFELMNPKLVSGVAMPDDEKK"
     gene            3694..4395
                     /gene="mrtC"
                     /locus_tag="VFQ61_26390"
     CDS             3694..4395
                     /gene="mrtC"
                     /locus_tag="VFQ61_26390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MrtC family glutamic-type intramembrane
                     protease"
                     /protein_id="HET9958064.1"
                     /translation="MTFDAVRKPLFALIGVTLLAAVLAQVLPEAHAATGVGLCFLGAT
                     YFLVWRHDTATIREFGLGLGGLFETEPLDPKKLASTATRALGWAFSLALIVLPGFAIG
                     YRLWYQPRGPFEAAPWLELLEQVPGQLLGTAFPEEAFYRGYAQSALDRAWPPKRRYLG
                     IALGPGVLLSSAVFAAGHFLTQPVPGRLAVFFPSLLFGALRARTGGIGASVVFHASCN
                     LFVWYLGRSYGLFRA"
     gene            4392..>4964
                     /gene="nadC"
                     /locus_tag="VFQ61_26395"
     CDS             4392..>4964
                     /gene="nadC"
                     /locus_tag="VFQ61_26395"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET9958065.1"
                     /translation="MTWCDAVLDEVVLGALREDLAGGDVTTEALVDPEQRGVARAVAH
                     QACVVAGGEVFARAFYLVDRALRVECLKRDGERAEAGEALLVVEGAARSILMGERTAL
                     NFLQRLSGVATLTRRFVDAIPAGTRLRIADTRKTTPGLRRLERVAVRAGGGFNHRNDL
                     GSAVLIKDNHIVAAGGVRAAVERARAFAPHT"
BASE COUNT          827 a         1616 c         1565 g          956 t
ORIGIN      
        1 ctacgatgac aatgagccag tgaacggggc tgagggatcc catcgatgca ttcctccgag
       61 cagagaccag cccggactag atttcgagac catagcacca ataccctgcc aagccatcga
      121 gaagccgtag ggcacgaggc gcggtcaacc tacaaggaaa actcggcctc gaacggcacg
      181 tcgacccgcg atagcagaac ctcgcgcccg taagtacgag ggcctacgcg cgcgccgaaa
      241 tcgacgcgca cgggcggtgg ttctccgccc aattgttcga gtacgacccg caccaagtaa
      301 cgcccgcgcg gcgccgttgc ggcctcgaga tacacgttct cgaagttgcg cccgcgacat
      361 tcgttgtttc taccggggca atctcgcgac ttcacgaggc cactcgcgac ggggcgcccc
      421 acctccacga gctcacccgc agggtcaatc acctgcaggt ctacgtccgc gcgcgcggca
      481 tcccatgcaa tcacgaactg aaccgcgccg gtctctacgc cgcaaccctc gtcgatgatg
      541 ccattgcagt tgttgtcgcg agcatcgaag caacgttcgg gtgcagtgct gacgcaggcg
      601 agctcagcct cgggcccacg cgcggcccca cccgcgacat cgtgaacgtc cccggataaa
      661 gccccggagc cgttcgtcgc agcgtgacca cccgcacaac aaggcagaca aagcgcgaag
      721 gccagcgcac tcacgcgcgc tggcctcgca cttcgattcg gctgttcttg cttcgagtta
      781 cttcttttcg tcatccggca tcgcgacacc ggacaccaac ttcggattca tcaactcgaa
      841 gtacgcggcc cgtgccagct gcccagtttg accctgctcg tcgcgcagcg ccaagagcgg
      901 cccttgttcc ttcatgaatt tcagggtgcg aatggcgccc tggcgcttgg cctcgtcgtt
      961 gcccttggcc atctgccaca ggcggtggcg cagaaccacg cgcgtgaagg agtgcgggcc
     1021 gttgtcgaac actaggttgt cgagctgacg aatcaacatg acgcgcgccc actcctgggg
     1081 agtcatgcgg atgctgagtc gggtaatggc ctcggcatta tccacgtact tgaacaacag
     1141 acccgactcg agatcctccg tcgaccaata accgaagctc tggtaccaga gctcgccgag
     1201 ctcatcgagc gtcgccttcg gcttgttcgc gtagagcgcg accgcgcggg ccgccgtctc
     1261 cggcgtgccg cccaggatca gggcgagagc agcgtcgttg acgagcacgt cgctgttcat
     1321 catctcgaac agcttggctt cgacgctctg atcgaagccg cccttcgcga tggcgcgggc
     1381 cacctggtgg cgggtggaga gagaagactc gggcgtcatc aacgacatga gcgcgggcgc
     1441 cgtaccgggc accgggcgct ggatcagcgt ctcgagcagg cacgcgcgac ggaactgatc
     1501 ggatttctcg ctgccgccgt actcctgaat tcgcttggcg acctcgagga agtcgtcttt
     1561 ttcggccacc caggcgaggg ctgcgcacgc ttccgagcgg gactgctcgt tgttttttac
     1621 gtccgcgacg tagtcgagca gccacttgaa cgcgcggtgg tcgcgccact cggagaagcc
     1681 ctgagccgcg ccgaccccca gcgcgcgcag cgacatgccg agaatggcca cgccaccttg
     1741 catcagacct tccatggtga cgtcatattc gtcacgttcg ttcttgcgcc ggttgagcga
     1801 cttttccagc acgccccagg agcgttgatc cttcatccag cccacgtagc gaagcgcgct
     1861 ctgcgccacg acgaactcct ccggcatggg cggctgctgc ccttccttcg gcagcggcac
     1921 gttcgggttc gcccacttgc gcaaggcgtc catgtccttg gtggactcca tggccgccag
     1981 cgcgcgtaga ccgttggcgt gcggcgaagg gagctcgtgg atccagaaga tcaccgcgtc
     2041 ttcggcttgc tccgcgatct gctgacgctt ctcggggtgc aacatcgcca gatcggcgat
     2101 catgcgcgag gcaatcacgc gctcattgtc gtcccgcttg agctccatct cccaatcgcg
     2161 ctgatcggag tagatcttca atgggtccat gcgtaggcgc ttggccagcg tgggcaccgc
     2221 ccgcacgtcg ccgatcgcgg ccaacgccac cgcggcgcgc cactggtagt gcgtgtgcgg
     2281 cttgctctcg atgtagctat agagcgcgtt gccgccgcgc ggatcgttca gaccctgacc
     2341 cccgttcttg gtgtcgtgga tcatgtcgaa gacttgcttg gtttggtacc aagcaagctt
     2401 ctcgtcgtcc gagacggagc gtagaccgag cacgagccct tccgtgccga tgccgtcccg
     2461 cagcgcctcc aggaacttct tgcggctgtc cttgtcggcc gtcttcatgg cgtcgatcaa
     2521 gggctcacgg gcgcgtgact ccgcgatgcg acccaggccg ggtgcagcct gacgggccac
     2581 gtcggggtcc ttgtcttgca ccaacttgat caggacgtcg gtccacttgg gctccgcttt
     2641 gtcgctgagc acggtggcga ccagctgacg cactgacggg ctctcgtcgt tggccatgct
     2701 cgcgagcttg tcgaggctca cgagatccac gatcttggct gcgtcgaagg ccacgccgcc
     2761 gcccaaacgc tgcacggtcg agaggtgacc gagacgatac aactgcatga tctcgtcgaa
     2821 ggcgcgggta tccccgagca ccacgagggc ccacgcgatc tgcggcttcg caccttttcc
     2881 ggcggtcttc aatgcagcta ggagggcatc tcgcgccggc tcgcccagcg gacgcccgta
     2941 ctcggcgagc gaggtggcgg ccatcgattg cactccttcc gacgggtacg agagcgcctt
     3001 caccgccaga gcaaccccct cgggatcctt tgcccaggcg agctgcttga gcgcctcgga
     3061 tcggagctcg tcgcttgctt cgctgccagc ccattgccgc cactgcggga tctgctcgtt
     3121 cttcggcagc acgaagatcg ccttcttccg atcctcagcc tgctccacgg tcagcttctc
     3181 ggcgtcttgc ttgaagccaa tcagcaagaa cgcggcgaga cccgcaaccg ccaagacacc
     3241 aatgagaatg acgactgggt tgaagcgccc cttcttgaag ttgccatcac ccatgtcgaa
     3301 gctgcctgga aagccgccgc ccggtgcggg tggcacggct gggccgtcgt catctggcgg
     3361 ccatttggcg agatacactt gcgatggggg cgggcgcgag gcagaaggcg gagggagatt
     3421 cggcaggttc ggttcgagca tgagtttttc cgcgagctct gggcaggctg gccgatcgca
     3481 ggtaacttgc gaactgccgc cttccgacgt gcgcggcgac cttaacacta ctcagactgg
     3541 atttcgcgcc tcgcgcacga ccggttgcgc agctcattgc gggcgacgtt gcgcgtcaca
     3601 tggaggctcg tcggaaagtt tccccatcgc cgcacatgac cccggatatc ctgcggcagc
     3661 ttctcgcggc cattcggcca agccgcgcca atcatgacgt ttgacgcggt tcgcaaacct
     3721 ctgttcgcgt tgattggcgt cactctgctg gccgcggtgc tcgcgcaggt actgcctgaa
     3781 gcacacgcag caaccggcgt aggtctgtgt tttttgggtg cgacctattt cctcgtttgg
     3841 cggcatgaca cagccactat tcgcgagttt ggtttgggtc tgggggggtt gttcgagact
     3901 gaaccgctcg atcccaaaaa gctggcttcg accgccaccc gtgcgcttgg ctgggcgttc
     3961 agcctggcgc tgatcgtgtt gccgggattc gcgatcggat atcgactttg gtatcagccg
     4021 cgagggccgt tcgaggcagc tccttggttg gagctgttgg agcaagtccc ggggcagctg
     4081 ctaggcacgg ccttccctga ggaggcattc tacaggggct acgcgcagag cgctctcgac
     4141 cgggcctggc cgcccaagcg gcgctacctt gggattgcgc tgggtccggg cgtactgctc
     4201 tcgagcgcag tctttgccgc aggccatttc ctcacccaac ccgtaccagg gcggctcgct
     4261 gtattcttcc cttcgctgct gttcggggcg ctgcgcgcac gcaccggtgg aatcggcgcg
     4321 agcgtggtgt ttcatgcctc gtgcaacttg tttgtttggt atctcggtcg aagttacggt
     4381 ctattccgcg catgacttgg tgtgacgccg tgttggatga agtcgtgctc ggggcgctgc
     4441 gcgaagatct cgcgggcggc gacgtgacga ccgaagcgtt ggtggatccc gagcaacgcg
     4501 gcgtggcccg agccgtggcg caccaagcct gcgtcgtggc gggcggagag gtgttcgcgc
     4561 gcgctttcta tctggtggat cgggcgctgc gcgtggagtg cctcaagcga gacggtgagc
     4621 gcgcagaagc cggcgaagcg ctcttggtcg tcgagggggc cgcacgctcc atcctgatgg
     4681 gtgaacgcac ggcattgaac ttcctgcaac gactgagcgg cgttgcgacc ctgacccgac
     4741 gcttcgtgga cgcgattcca gcgggcacgc gtctgcgtat cgcggatacg cgcaaaacga
     4801 cgccggggtt gcgacgtctg gagcgagtcg cggtgcgcgc cggtggcggc ttcaatcatc
     4861 gaaatgacct cggctctgcg gtgctgatca aggacaatca catcgtcgcc gcgggcgggg
     4921 tccgagccgc tgtcgagaga gcccgcgcct ttgccccgca caca
//
LOCUS       DASTKU010000757         6708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_594448, whole genome shotgun sequence.
ACCESSION   DASTKU010000757 DASTKU010000000
VERSION     DASTKU010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6708
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594448"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VFQ61_26400"
     CDS             <1..764
                     /locus_tag="VFQ61_26400"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HET9958066.1"
                     /translation="GTQAYMAMEKVLSVVGDPRYDLIVLDTPPTSDALDFLDAPERLI
                     GAIDSAALGWLTQAFQKTGFFSLNVLAKGVSLVLRGLSRLTGQGFLEHLAAFVGDLNE
                     LFGGFRERAERVARAFRAPDFAYLLVATPSAEALGEAAFFAERLQRLGMRADALIVNR
                     TRPKPSSAPVPEQIASRLRGHGSSASSDLVMRILRASGEESASFERERRAVDGARARA
                     SALSRERVPLCVEVPAFPESVHDVAALVSACRHLA"
     gene            complement(840..2159)
                     /gene="der"
                     /locus_tag="VFQ61_26405"
     CDS             complement(840..2159)
                     /gene="der"
                     /locus_tag="VFQ61_26405"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET9958067.1"
                     /translation="MTPIVALVGRPNVGKSTLFNRLAGKNLAIVHDMPGVTRDRHYAL
                     AHIGGRNVTLVDTGGFDPTDEDPMKAGIARHVNAAIEEADVVVCVLDGSAPPTGPDRD
                     TVQLLRSSGKPVVYVANKVDAPKHALEVGPLYELGLPELFTVSALHGRGTGELGLAIR
                     ERLPDVSEEEDEPGELPRIALIGKPNAGKSSLFNCLTGQERSLVDHRPGTTRDPVDQA
                     IEFEGESFILVDTAGIRRRSRVEPGVELVSVLRAIRAVERAEVCILMCDATEGVTEQD
                     ARLLSLCLERRRAVVVGLNKVDLLDGKAKQRALEQAEETLRFAGFAEIVQLSAKQGKN
                     VKGLMRAAARAAQEMRRRIPTSELNRFFEEVLATHPPPTDSGRAPRMYYITQAESSPP
                     TFVIMSNFPENIKESYQRFVINQIRQAFSFRGVPIKVNYRAKRRDSE"
     gene            complement(2156..3088)
                     /gene="era"
                     /locus_tag="VFQ61_26410"
     CDS             complement(2156..3088)
                     /gene="era"
                     /locus_tag="VFQ61_26410"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET9958068.1"
                     /translation="MRFGTVALFGRTNVGKSTFLNAVLEHDLAIVSALPQTTRDALLG
                     VVTRPDVQLAFVDTPGIHRPKSELGRRMNHAAREAARTTDAILFMTDLGGHPPGTKPK
                     DPIAPEDRELLKDLPKDGGERVVLGINKVDTLRDKGRLLPLLSAFQEAYPFKALVPLS
                     ARTGDGVDRVINELIAVLPEAPAAYPADTLTDRPASFFVREFVREQVLAATRGEVPHA
                     VAVSIDRYEEGRVARIAATLHVEKVGQRKILVGEGGLTLRDIGTNARKRIEELLGQRV
                     HLELFVRVTPKWKDVPRQLADLGYMGSTKRGENR"
     gene            3192..5720
                     /locus_tag="VFQ61_26415"
     CDS             3192..5720
                     /locus_tag="VFQ61_26415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET9958069.1"
                     /translation="MAADLSRTEPGTPASRALRWGTLVFAGCSLGAILSPGLWDPVEL
                     ERVEWARRIAVGLLGASEYASPGDSPLPSLSELGQGELPFLLMACGLRIFGLHAWAAR
                     LPLALCALVGLGGLFFLVDRLENRRAAWLSVLAVACTPAYFLQARTLTGEIVTFAAVT
                     LASAGLGVLGFDVSATLWARIAAWVCVVTGLAVGLATRGALLGIAVPVLGFGLTSLAL
                     ALAGVRVLDPRFHRSARGFELTALGPLLSLGGVVVALWGARAVSETPPERYSRWVGAL
                     TGGSHPIPTFDVTFRDLGHGLLPWSAVLPFALARVLARCKSPRATRFEPDLALRMQLV
                     CTSGVALLLHSALTKEFGVLPFCGTVPLIAIAPLSLLDLEERAERATAWAVATAALLV
                     LLLLDFLHLPEKGLAPLGLSEVEFPAAFVGTAFLRLAPILAFVGVSWFYIVAGRETVD
                     SAEKGAVSWFAPAEYLRWPRVVRRLWLGNLGVTLVTIFGALLAFDLIQSLSERYLGLA
                     AFGSISEISVLLVRAAWLGLLSFLIGPELVLLGRDVTSWVLAQLGAQRATAAVFVFAS
                     GGALLGLVYYRPLFEQLRGGEVFAAYARRAAPGEPLGVYGSPRSALRYEAPRAEANLF
                     DDLESAIGWLCADKERRFLSLGLEELAFANSAFRSCTTPRRNLAVLSLSTGRFVLASN
                     RAKPSDTDRNPFASVVLSTAPAPDHPLDVDLGGLVHVMGWELRDASGNVVRALRTGVD
                     YEFLAFYRVMTVPRGNWKAFVHIERLQQRLNADHALLDGKYPTTLWMPGDILVDKHRF
                     RLRRDYPSGVARVYFGLYSGERRLEVRRGEHDADRVSGGTLEVL"
     gene            5809..6195
                     /locus_tag="VFQ61_26420"
     CDS             5809..6195
                     /locus_tag="VFQ61_26420"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HET9958070.1"
                     /translation="MSRDDSERPPPMSERRSAGRLDVTWSVDCETEDTFLYANITNIS
                     EVGIFVRTQDPLEVGTRLTLKFSPPSESESLVLVGQVQWVNPVRMLGDNPNPGMGIRF
                     VDLTPEARERLIDIVHTIAYVRSSSN"
     gene            complement(6202..>6708)
                     /locus_tag="VFQ61_26425"
     CDS             complement(6202..>6708)
                     /locus_tag="VFQ61_26425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958071.1"
                     /translation="SDRSYDQALAAYRDRRYAEAEKRFAEVAERGEENAASAALYSAL
                     ATRARAGCSAAAPRFDAVHQKYPDAGAGREAAWQSAGCYRTLGDFEKARRQYEGLLGL
                     PGYADRAQAALGSLGDTENSELAARRAAAAAAAKPAGESPAKAAGAATRPAAPVDEAS
                     EGRAKGSH"
BASE COUNT          978 a         2132 c         2223 g         1375 t
ORIGIN      
        1 cgggtacgca ggcctacatg gcaatggaaa aggtgctcag cgtggtcggt gatccgcgct
       61 acgacctaat cgttctcgat actccgccca ctagcgacgc gctcgatttc ttggatgcgc
      121 cggagcgttt gatcggtgcg atagacagcg ccgcactggg gtggctcacg caggcatttc
      181 aaaagaccgg cttcttcagc ttgaacgtgc tcgcaaaggg cgtgtccctc gtgctgcgag
      241 ggttgtcgcg ccttactggt caaggctttc tcgaacatct ggcggcgttc gtgggcgacc
      301 tgaacgagtt gttcggcggc tttcgcgagc gcgcagagcg agtggcccgc gcgtttcgtg
      361 cgcccgactt cgcgtacttg ctggtggcca cgccgagcgc tgaggcgctc ggcgaagctg
      421 cttttttcgc ggagcggctg caacgcttgg gcatgcgcgc agacgccttg atcgtgaacc
      481 gcacgcgacc caaaccgagc tcggcgccgg ttcccgagca gattgcgagc aggctgcgcg
      541 gccatggctc gagcgcgtct tcggatctgg tgatgcgcat cctccgtgcg agcggggaag
      601 agtccgcgag cttcgagcgc gagcgtcgtg ccgtcgatgg cgcgcgcgct cgcgcgagcg
      661 cgttgtcgag ggagcgcgtc ccgctctgcg tcgaggtgcc cgcgtttccg gagagcgtgc
      721 atgacgtggc cgccctcgtc tcggcttgcc ggcacctcgc ctgagtggcg cgcccgaact
      781 gcggcggccc gcgtcaagcg ttcgcgctac accgctggac ctagccctct cttctgccgc
      841 tactcggaat ctcgccgttt ggcgcgataa ttcactttga tcggtacacc gcggaagctg
      901 aacgcttgcc ggatctggtt gatcacgaag cgttggtagc tctctttgat gttctccggg
      961 aagttgctca tgatcacgaa ggtcggcggc gagctctcgg cctgggtgat gtagtacatg
     1021 cgcggcgccc gcccggaatc cgtaggtggt gggtgggtgg cgagcacttc ttcgaagaag
     1081 cgattgagct cggaggtggg aatgcgacgg cgcatctcct gagccgcacg cgcggccgcg
     1141 cgcatcagcc ccttcacgtt cttaccctgc ttggcgctga gctgaacgat ctccgcaaag
     1201 cccgcgaagc gcagggtttc ctccgcttgc tcgagggcac gttgcttggc tttgccgtcc
     1261 agcaaatcca ctttgttgag cccgaccacg accgcacgac gtcgctccag acacaacgac
     1321 aaaaggcgcg cgtcttgttc ggtcacgcct tcggtcgcat cgcacatcaa gatgcacact
     1381 tcggcgcgct ccacggcgcg aatcgcgcgc agaacgctga cgagctcaac ccccggttcg
     1441 acgcgcgagc gacgccggat ccccgccgtg tcgaccagga tgaaggattc tccttcgaac
     1501 tcgatggctt gatccaccgg atcgcgcgtc gtgcccggac ggtgatcgac gagcgatcgc
     1561 tcttgtcccg ttaggcagtt gaacagcgaa gacttaccgg cgttgggctt gccgatcagc
     1621 gcgatgcgcg ggagttcccc cggctcatcc tcttcctcgc tgacgtccgg caagcgctcc
     1681 cggatggcca ggccgagctc gcccgtgccg cggccgtgca gcgcagagac ggtgaacagc
     1741 tcggggaggc cgagctcgta gagcgggcca acctcgagcg catgcttggg cgcgtccact
     1801 ttgttggcga cgtacaccac gggttttccg ctgcttcgca gcagctgcac cgtgtctcgg
     1861 tcggggccag tcggcggcgc cgagccatcc agcacgcaca ccacgacgtc ggcctcttcg
     1921 atcgcagcgt tcacgtggcg tgcgatgcca gccttcatcg gatcttcgtc agtcgggtcg
     1981 aagccgcccg tgtcgactag cgtgacgttg cgcccgccga tgtgcgcgag cgcgtaatga
     2041 cggtcgcgcg tcacgcccgg catgtcgtgc acgatggcca ggttcttgcc cgccaaacgg
     2101 ttgaatagcg tgctcttgcc cacgttgggg cggcccacta gggcaacgat tggcgtcatc
     2161 ggttctctcc gcgcttggtg gagcccatgt atccaagatc cgcgagttgc ctcggtacgt
     2221 ccttccattt gggcgtcacg cgcacgaaca actccagatg aacgcgctgt ccgagcagct
     2281 cttcgattcg cttgcgagcg ttggtcccga tgtcccgcag cgtcagccca ccttctccga
     2341 ccaagatctt gcgctgcccg accttctcga cgtgcagggt cgccgcgatg cgcgcgacgc
     2401 gaccttcctc gtaacgatcg atgctcaccg ccaccgcgtg cggcacttca ccgcgggtcg
     2461 ccgcgagcac ctgctcgcgc acgaactcgc gcacgaagaa gcttgcgggg cgatcggtga
     2521 gggtatctgc cggataggcc gcgggcgcct cgggcaatac agcgatcagc tcgttgatca
     2581 cgcgatctac gccgtccccc gtgcgcgcac tgagcgggac cagggctttg aaggggtagg
     2641 cttcctgaaa cgcggagagc aacgggagca gccgcccctt gtcgcgcaac gtgtccacct
     2701 tgttgatgcc cagcaccacg cgctccccgc catccttggg cagatctttg agcaactctc
     2761 gatcttcggg cgcgattgga tccttgggct tggttcccgg cggatgtccg ccgagatcgg
     2821 tcatgaacag aatggcgtcc gtggtgcggg cggcttcacg ggccgcgtgg ttcattcgcc
     2881 gtccgagctc cgacttcggg cgatggatgc cgggcgtgtc cacgaacgcg agctgcacgt
     2941 cgggccgcgt gaccacaccg agcagggcgt cacgggtcgt ctggggcaac gccgaaacga
     3001 tggccaagtc gtgctccagt acggcgttca agaaagtgga cttgccgacg ttggtgcggc
     3061 caaataaagc aacggtaccg aagcgcattt cgagccgcgg gggctacccc cggtagcgcc
     3121 tcctggcaag cggccagtgt aaacgcgctg ctgcatacct cacggcttcc ggttaaaaga
     3181 gccatacccg tatggcggcg gatctttccc gaactgagcc cggaacgccc gcaagtcggg
     3241 ctttgcgctg gggaaccctg gttttcgccg gctgctcact cggcgctatc ctcagcccgg
     3301 gcctctggga tccggtcgaa ctcgagcggg tggagtgggc gcggcgtatc gcggttgggc
     3361 tactcggggc ctccgagtac gcgagcccgg gcgattcgcc gctgcctagc ctgagcgagc
     3421 tcggccaagg cgagctgccc ttcttgctca tggcctgcgg cttgcgaatt ttcgggctcc
     3481 acgcctgggc ggcgcggctt ccgctggcgc tttgcgcgtt ggtgggtctg ggcggcctgt
     3541 tctttctggt cgatcgactc gagaatcgtc gcgcagcctg gctgtcggtg ctcgcggtgg
     3601 cttgcacacc tgcctatttt ctccaggccc ggacgctcac gggagagatc gtgacgtttg
     3661 cggccgtcac gctagcgagc gcggggctcg gcgtgctcgg gttcgatgtc tctgcgacgt
     3721 tatgggcgcg cattgcggct tgggtttgtg tcgtgacagg actagcggtg ggcctcgcga
     3781 cgcgcggggc tttgcttgga atcgccgtgc cggtgcttgg ctttggcctt acgagcttgg
     3841 ctctcgctct ggccggggta cgcgtcctcg atcctagatt tcatcgatct gcgcgcgggt
     3901 tcgagctcac ggcgcttggt ccactgttgt cgttgggggg agtcgtcgtc gcgttatggg
     3961 gggctcgcgc tgtctccgaa actccgcccg aacgctattc acgttgggtg ggcgccttga
     4021 cgggcggctc ccatccgatc ccgacgttcg acgtcacgtt ccgggatctc gggcacgggt
     4081 tgcttccctg gagcgcggtg ctgccgtttg ctctggcgcg tgtgttggca cgctgcaagt
     4141 cgccgagggc cacgcgcttc gagccagacc tggccctacg aatgcagctc gtctgcacgt
     4201 ccggcgtggc gttgttgttg cacagcgccc tgaccaaaga gttcggggtg cttccatttt
     4261 gcggaacggt acccctaatc gcgattgcgc cactcagctt gctggatctc gaagagcgcg
     4321 cggagcgagc cacggcctgg gcggtcgcca cggccgcgct actggtcttg ttgctcttgg
     4381 atttcctaca tctcccggag aagggcctgg cacctttggg gctgtcggag gtcgagttcc
     4441 cggccgcctt tgtcggcacc gcatttctgc gtttggcccc gatcctagct ttcgtgggcg
     4501 taagctggtt ctacatcgtc gccggccgag agacagtcga ctcggcggag aagggggcgg
     4561 tgagctggtt cgcacctgcc gagtatctgc gctggccgcg ggtcgtgcgg cggctatggc
     4621 tcggcaactt gggggtcacc ctggtgacga tcttcggggc gctgctcgcc ttcgatctaa
     4681 tccagagcct ttccgagcgg tacttgggtt tggctgcgtt cggtagcatc agcgaaattt
     4741 cggtcctgct ggtgcgggcc gcttggttgg gcctgctgtc atttttgatc ggaccggagc
     4801 tggtgctgtt gggacgcgac gttacgtctt gggtcctggc gcagcttggc gcgcaacgtg
     4861 cgacggccgc ggtgttcgtg tttgcaagcg gcggagcttt gctcggcctg gtctattatc
     4921 gaccgctgtt cgagcagctc cgaggtgggg aagtgttcgc ggcgtatgct cgacgggcgg
     4981 ctccgggtga accgctcgga gtgtacggta gcccacgctc ggcgctgagg tacgaagctc
     5041 cacgcgcgga ggcaaacctg ttcgacgatc tagagtcagc cattggttgg ctgtgtgcgg
     5101 acaaggagcg ccgctttttg tccctcggct tggaagagtt ggctttcgcg aactccgcat
     5161 tccggagttg caccacgccg cgccgcaacc tcgcggtgct aagcctgtcc accgggcgct
     5221 tcgtgcttgc gagcaatcgc gcgaagccga gcgacaccga tcgcaatccg ttcgcctccg
     5281 tcgtgctgtc gactgctccc gcgcccgatc acccgctgga cgtggacttg ggcgggctcg
     5341 ttcacgtgat gggttgggag ctgcgagacg cctccggaaa cgtagtccgc gcgttgcgaa
     5401 cgggggtcga ttacgagttc ctcgcgttct accgcgtgat gaccgtgcct cgcggcaact
     5461 ggaaagcatt cgttcacatc gagcgtttgc agcagcgctt gaacgcagat cacgcgctgc
     5521 tcgacgggaa gtacccgacg accttgtgga tgccgggcga tatcctcgtt gataaacatc
     5581 ggtttcgtct gcgtcgcgac tatccgagcg gcgtcgcacg cgtctacttc ggcttgtata
     5641 gcggcgagcg tcggctggag gtccgacgcg gtgagcacga tgcagatcgc gtttcgggtg
     5701 ggaccctcga ggtgctttga cccatctgcc cgcttctgtc ctacttgatt gttggtttgg
     5761 gtgggcgggt gctacccatc gttcatcgag cagtgctaag cggagcgagt gagtcgcgac
     5821 gattccgagc gtcctccgcc catgtccgaa cggcgcagtg cgggccgcct cgacgtgacg
     5881 tggtcggtcg attgcgagac cgaggatacg ttcctgtatg cgaacatcac caacatctcc
     5941 gaggtgggga tcttcgtgcg cactcaagac ccgctcgagg tcggcacgcg cctgacgctc
     6001 aagttttcgc cccccagcga gtccgagtcg ctcgtgctcg tcgggcaggt gcagtgggtc
     6061 aatcccgtgc gcatgctcgg tgacaatcca aacccgggca tgggcatccg cttcgtcgac
     6121 ctcacaccgg aggctcgcga gcgcctaatc gacatcgttc acacgatcgc ttacgtgcgc
     6181 agctcttcca actgagccag ctcagtggga gcctttggct cgtccctcgc tagcttcgtc
     6241 aaccggcgcg gccgggcgag tcgcagctcc ggcggctttc gcggggcttt ctccagcggg
     6301 tttggcagcc gcggcggcgg ccgcgcgccg cgctgcgagc tcgctgttct cggtgtcgcc
     6361 gagagatccc aacgcggcct gcgcgcgatc cgcgtagccg ggcagtccca ataggccttc
     6421 gtattggcgg cgggcttttt cgaaatcccc tagagtccga tagcaacctg cggattgcca
     6481 cgctgcctct cgaccggcgc ccgcatcggg atacttctgg tgtacggcat cgaaccgggg
     6541 cgctgcggca gagcagccgg cgcgcgcgcg cgttgcgagc gccgaataca aagctgcgct
     6601 cgccgcgttc tcttcaccgc gctccgcgac ttcggcaaag cgcttctctg cctcggcgta
     6661 acgccggtct cgatacgccg cgagcgcctg atcgtagctg cgatcgct
//
LOCUS       DASTKU010000758        11494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_370855, whole genome shotgun sequence.
ACCESSION   DASTKU010000758 DASTKU010000000
VERSION     DASTKU010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11494
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370855"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1754)
                     /locus_tag="VFQ61_26430"
     CDS             complement(<1..1754)
                     /locus_tag="VFQ61_26430"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HET9958072.1"
                     /translation="MRRAVRMDAVTLRGSRWGAMILLGFAGGCEGLIGASFEGAHSVA
                     AESQGDAGAAPDLGGRSGRDDASGGAEAGSISSAGDHANGGASAGEGGQVESAGGRAE
                     ATGGRAQATGGHAQATGGLAGVNTGGTGGKLNDCSAGVAFHDLAFSPEDEPVPVAFER
                     EIPAEVVAPGIGIAIGVSPPCIGAKISTDKLLFADCNAQQNQKVRVVEGGKTGALTFA
                     DHPISAPEEQIRFSILNLNPWGENRPVAWSMRSPQPGEPVTLFGWDKDRIRVAHTTLQ
                     PAAFEGPVECDGCPRPGQETPMMLAFGGDARLVAFCKLNACGGVSDCLAIEGTRSWDK
                     LSPFATQQGLFFSDLDGDGKADALAVARKQLRARLAGTSQFLELREWFAGEASGDAAN
                     ALGDVTGDRRADFISIGNGISIYPAIGDEFGAARLLSAASTSALGARVGDVDGEGSGE
                     VVIWQPRKILVIRDANTPLPRFETWYGALPAGVDNLDVRDVNGDGRADLVLGFADHVA
                     VLPSTGSAFAEPEDWLTVDTTPPGWFFADVTGDGRADAIQIDLRGAGVYRSTGSAFVH
                     EDTFRTLPIGERGNYFADV"
     gene            complement(1796..2302)
                     /locus_tag="VFQ61_26435"
     CDS             complement(1796..2302)
                     /locus_tag="VFQ61_26435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958073.1"
                     /translation="MKWRELLGASFILVSPVLGYLTARQPYAEALSNLRWAFWTLLAA
                     PALLGIQLVVYRIVSGVRWANMGPAYVVIPFFALWTAGLLVRTLDQTATQIRVAEYSS
                     TVLEVRGGKRPEIVLAPFANSTSPVSVLQSEARLFNPPIGTRVRIIDEAGPLGIGTRQ
                     FIRADEAL"
     gene            complement(2346..3512)
                     /locus_tag="VFQ61_26440"
     CDS             complement(2346..3512)
                     /locus_tag="VFQ61_26440"
                     /inference="COORDINATES: protein motif:HMM:NF021456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HET9958074.1"
                     /translation="MSPGRTSAVNVVDYAGPHPVVGVVAKRTYSVNGGLVRLADEQLP
                     LVEEPRLQPTSELLEADIDVMLRRPLVDVIVKGHAYAPSAKATRFTAGLRVGALSRKF
                     IVTGARRVERDMMGELRFTPAEPIAKVPLSWELAYGGADGAFARRHLDVEATLAALRL
                     TKMVPSAKTVEDALCGYPRNPAGRGYLLEATPESLERFELPQIEEAFEPLIPETLVRK
                     SPDYWPTAPLPAVTGFLPYVFFPRSLDCGLPAMPYDLDSVRPEHFPEVRLGLVSPRAV
                     QVGMHASERVSLQAGQSAAPGMRLPKLLPGERIALESLHPQNRAWEFTLPAERPRLIY
                     RRPGNAAVEVEAQIRMLLIEPDESRMTVVWTGEAQEAALSEQQFLQIEHAVLWT"
     gene            complement(3509..4399)
                     /locus_tag="VFQ61_26445"
     CDS             complement(3509..4399)
                     /locus_tag="VFQ61_26445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958075.1"
                     /translation="MFPFPGGKPCIAQWIPILGVDLHFTLPPIPPPPAPPVPQPHLIV
                     WLEGLSCAPGSSKVANTGNGAKNVLSPIGALVGRGHDAAFVVAHICLTMPLHALLPVI
                     ILGSANKVEFGVASVRLPTGPVSVGESFVVGTQLDCSEPFPLPSSKNISILNYTVQAG
                     LTMADIIASLAAMLVDGLITFILGKIAGGLVKGLGKLAERFVVSLGLRATLIVVIIGM
                     RMARSPFVIRALGPQAAAGVTRILEGLMGVALGGPLGWTHPYAPWRLVPSNYQSSDIV
                     FDTVQRPVSKLEQSVKGLFQ"
     gene            complement(4399..5376)
                     /locus_tag="VFQ61_26450"
     CDS             complement(4399..5376)
                     /locus_tag="VFQ61_26450"
                     /inference="COORDINATES: protein motif:HMM:NF021456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HET9958076.1"
                     /translation="MQIANSTPAQIRLLKSSFPPDPLVYAAAIVKLTFEIGADGRTAI
                     ASEQMPLQAEFLETPHGVLHGDMYPKKRGVDVCVFATVQCSRPTTQTMVWLTTRERRV
                     GLRVTGNRHWQVGTTGDPVPSAPEPFTNMPLTYANAYGGLRQEELGLVAFPDNPHGKG
                     ALLRDARDSLGKPLPNIEPAEGPFVSRWNDVGVVAGFGPYPMVWGLRAREGVEVNLDA
                     GEVEGIRPSLFNHAHPSLVLNQCEPGDVIVLEGAANAASRLSIPRVQPKLDISVGENQ
                     GSLLPGLDGVFWWVSERRLVLTYRGRFQYGFVREEPRRAELTLLSQESS"
     gene            complement(5367..5951)
                     /locus_tag="VFQ61_26455"
     CDS             complement(5367..5951)
                     /locus_tag="VFQ61_26455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958077.1"
                     /translation="MLKDTKRQPERQLVEQLARSVPTASEEIPIGHSRVATLSATPEG
                     AHLCVRAPSGAAELELELVTSEAGTTVRVRATAIAIQAQDIAAECRTFSVKASERIEL
                     SSGGELRETALTLTETAERIQVDATRGSVEVRANDDVQLLGERVLLNCDPPAPVPPWL
                     AGTLQISPGNSVRFLGDQPAAPVTDSEGEGSGCR"
     gene            complement(5977..8109)
                     /gene="tssI"
                     /locus_tag="VFQ61_26460"
     CDS             complement(5977..8109)
                     /gene="tssI"
                     /locus_tag="VFQ61_26460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HET9958078.1"
                     /translation="MVRVAEVLSVLDEGALLLLSLRGSEALSRPFSYEVDLLSENEKI
                     DFGALLGQPMGVLLELQGQSQRQFIGIVTEFAMVGGLGRYVRYRAVLRPWIWLLSKRT
                     NCRVFQRRSVPDIVKEIFREHGFSDFDDRLTHHENYPVLDYVVQYRESDLNFVSRLLE
                     HVGIYYFFRHGNGKHTLVLADSYSAHEPAPDAKLIRYMPPQQGQRRETEHFDQWSFSR
                     RIRSGSYVGRDFDFEKPRAQLTPQIARPQEHAHSNYEQFDYPIDFVERSQGESRVQMR
                     LEEQHVEYETVSAAGNVRALNAGHLFTLEEFPRADQNKEYLTLETAYALQVNGYESSA
                     GTDSELTYRVDIRALDSKRQFRPARITPKPVIEGPQTALVVGDDPNEIWTDSHGRVKV
                     QFHWDRLGKFDGDSSCWVRVSQLWAGSGFGGIHIPRIGQEVIVDFLEGDPDRPIITGR
                     VYNQLNKPPYELPLNKTQSGIKSRSTLRGLDSNFNEIRFEDKKGQEELFIQAEKNQVT
                     KVKVNQSVSVGANQSISVGANQSVAVTANQSVTVTGERTVDITKKDKETSHALRENFY
                     LAERHTQVTTLEKLDVIGDYKTGVTQAYTLQGDGKIELNHASDQLKMEKALVHLKNET
                     CELKLDGGDVTLKADSKLTLQCGGASITLTKDGTIEISGTQKANITGFKEVTVVGSTK
                     VNIAGGGSKVDLDSGGATVTGTAVNLNT"
     gene            8368..9102
                     /locus_tag="VFQ61_26465"
     CDS             8368..9102
                     /locus_tag="VFQ61_26465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958079.1"
                     /translation="MESAPNAGAPAPLESDECHLNEELDALFGARENAAELEATFRAR
                     LLPTNEAARRGRPLAHRVVTLIEGRRFRELASLINPERGLCLRAAKGAECRTLSRAEL
                     IECGSSPTQERWAVDTAADERPLLTCSRAFERIFFVRGFRSPDAVTFNCFPPLGRANN
                     GSTILQQPASVYVELRLNERENSSGGDEGRFRALWLVFDGPPDALEWVEITAEYPGMQ
                     RDVRIRGSSAPCLHRDPSVCHEDYGT"
     gene            complement(9090..10859)
                     /locus_tag="VFQ61_26470"
     CDS             complement(9090..10859)
                     /locus_tag="VFQ61_26470"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HET9958080.1"
                     /translation="MPTRTRAGMAIATCASGYIACNAIEPLELGSQRQALAITQSFQR
                     GVSPGSGYLTTADAMLQQSAPDQNLGAATTCTADGDERGGLDSSCLFYWDVSAIPPGS
                     VITEARISLRVTKGSNNVYDVHELLRPWSESQVTWNSARAGSPWGAPGALANTDRGAR
                     LGTITGSTNSTQTVILNAAGIARVQAWVDNPSSNAGLIVANATNPKELALATGEHATL
                     AYRPTLAITYETSGGGGSGGSTGAGGSATGGSATGGANSGGSAGTSGSGGSSGGGGVP
                     GATEPDLLVAFIGDQGANTNADAVLNLIKNEGAAAVVHNGDFDYANNPTAWDDRITRI
                     LGADYPYFALIGNHDAAAWGGTSGYAAKIAARHARVPEMNCAGELGVKATCNFRGLYL
                     VESCVGVTEWSTTRCAKDALEQVDFLRSSLAADQSLWSVCNWHKNQNDMQVGTKTDEV
                     GWNAYRECMLGGALVATGHEHSFSRTLALTDVGNRSNAHGATGLFDTIQLAPGRNFVF
                     TSGLGGVGIRPFDTTTHGDDTWWASYYTSDRWFRNGVLQTGAGTFGAFFIRFHVGGDP
                     KRADAYFKDVNGRIADQFTIQVP"
     gene            complement(11154..11372)
                     /locus_tag="VFQ61_26475"
     CDS             complement(11154..11372)
                     /locus_tag="VFQ61_26475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958081.1"
                     /translation="MTAHTTSNPAFESTLRLARTRAPAAGLTIWFGLALLGASYLDLR
                     GPVPAPSAASCASRVEAAQEIVTSAFDR"
BASE COUNT         2146 a         3650 c         3517 g         2181 t
ORIGIN      
        1 acgtcagcaa agtagttgcc acgctctccg atcggcaacg tgcgaaaggt gtcctcgtga
       61 acgaaggcgg agcccgtgga acggtagact ccggcacccc ggaggtcgat ttggatcgcg
      121 tcggcacggc catcgccggt gacatccgca aagaaccagc ccggcggcgt cgtgtcgacc
      181 gtgagccagt cttcgggctc cgcgaaggcg ctgccggtgg aagggagaac cgcaacgtga
      241 tcagcgaagc ccaggacgag atcggcgcgc ccgtcgccgt tgacgtctcg aacgtccaag
      301 ttgtcgacgc cagccggtag cgcgccgtac caggtctcga agcggggaag cggcgtgttg
      361 gcatctcgga tgacgaggat ctttctcggc tgccaaatca cgacttctcc gctgccttcg
      421 ccgtccacgt cgccaacacg ggctccgagc gccgacgtcg aagcggcgct gagtagtcga
      481 gccgccccga actcgtcacc gatcgccgga tagatgctga tgccgttgcc aatgctgatg
      541 aagtcggcgc gccggtcgcc agtcacgtct cccagcgcgt tcgccgcgtc gccgctcgct
      601 tcacccgcga accattcccg caactccaag aattgactcg tccccgcgag tcgagcccgg
      661 agctgtttcc gggccacggc gagcgcgtct gccttgccat ccccgtccag atcggaaaag
      721 aacaatccct gctgggttgc gaatggcgag agcttgtccc agctgcgggt accttcgatg
      781 gccaagcaat cgctcacacc gccacaggca ttcaatttac agaaagccac gagcctcgcg
      841 tctccgccga aggcgagcat cattggcgtt tcctgtcccg ggcgcggaca accgtcacac
      901 tcgaccggac cctcgaacgc ggccggttgc agcgtcgtgt gcgcgacccg aatgcgatct
      961 ttatcccaac caaagagtgt caccggttcg cccggctgcg gcgagcgcat ggaccacgcc
     1021 acggggcgat tttcacccca gggattgaga ttgaggatac tgaaacgaat ctgctcctcg
     1081 ggcgcggaga tagggtgatc cgcgaacgtg agcgcgcccg ttttgccgcc ctcgacaacg
     1141 cgcacctttt ggttttgctg ggcgttgcaa tcggcgaaca acaacttatc ggtggagatt
     1201 tttgcgccaa tgcagggtgg gctcactccg atcgcgatgc cgataccggg tgccacgacc
     1261 tctgccggga tctcacgctc gaacgcgacc ggcaccggct cgtcctctgg actgaaagca
     1321 aggtcgtgga aggccacgcc cgcggagcaa tcgttcagct tgccgcccgt accgccggtg
     1381 ttgacgccgg ctagccctcc ggtggcctga gcatggccgc ccgtcgcctg ggcgcgacct
     1441 ccagtcgctt cggcacggcc gcccgcactt tcgacttggc cgccctcgcc tgcagaagca
     1501 cccccgttcg cgtgatcccc ggccgacgag atactccctg cctcggcgcc accggacgcg
     1561 tcatcgcgcc cgctcctacc cccgaggtcc ggtgcggcgc ccgcgtcccc ctggctctct
     1621 gcggctaccg agtgtgcgcc ttcgaacgaa gcgccaatga ggccttcaca acccccggcg
     1681 aaaccaagca ggatcatcgc cccccagcgt gagccgcgta aagtcaccgc atccatccga
     1741 accgcgcgcc gcatgcaccg cagtcaaaca ctgcgccagc cgagaaacaa gcgtctcaga
     1801 gcgcttcgtc agcacggatg aattgacggg ttccgattcc aagtggcccg gcttcgtcga
     1861 tgattcggac gcgggttccg atcggcgggt tgaataggcg agcttcggat tggagaacgc
     1921 taacgggtga agtggagttg gcgaatgggg caagtacgat ttcggggcgc ttgccgcccc
     1981 gaacctcgag gacggtgctc gagtactctg caacacgtat ttgggtggcg gtctggtcga
     2041 gggttcgcac gagcagcccg gctgtccaca gggcgaagaa ggggatgacg acgtaggccg
     2101 gacccatgtt ggcccagcga actccggaga cgattcgata gaccacgagc tggatgccca
     2161 gcagtgcggg tgcggcgaga agtgtccaga acgcccagcg aagattagat aacgcttcgg
     2221 cgtaaggctg tcgggccgtc aagtagccca ggactggcga gaccaaaatg aacgaggcac
     2281 ccagcagttc tctccacttc atgaccgaca tcttcttctc aggcagttga gtgctcgaat
     2341 tgcgcttagg tccacagcac ggcgtgttca atctgcaaga actgctgctc cgagagcgcg
     2401 gcttcttggg cttcgcccgt ccacacgacg gtcatccggg attcatcagg ttcaatgagc
     2461 agcatgcgaa tctgcgcctc cacttcgacg gcggcgttac cgggccgcct atagatcagc
     2521 cgcggccgct cggcaggcag cgtaaactcc caggcccggt tctgcggatg aaggctctcg
     2581 agcgcaatgc gttcgcccgg tagtagcttc ggcaagcgca taccgggcgc ggcggactgc
     2641 cctgcctgga ggctcacgcg ttcgctcgcg tgcattccaa cctgcaccgc tcgcggcgac
     2701 accaatccga gccgcacttc cgggaagtgt tctggacgca cggagtcgag atcgtaaggc
     2761 atggcgggca agccacagtc caggctgcgc gggaagaaga cgtagggcaa gaagccggta
     2821 acggcaggca gcggcgcggt gggccagtaa tcagggctct tgcgcaccag ggtttccgga
     2881 atcaagggct cgaaggcctc ctcgatttga ggcagctcga agcgctcgag tgattcgggc
     2941 gtcgcctcta ggagataacc acggcccgcg gggtttcgcg ggtatccgca cagcgcgtct
     3001 tcgacggtct ttgcgctcgg gaccattttg gtcaggcgca acgccgcgag cgtcgcctcc
     3061 acatcgaggt gacgacgcgc gaacgcgccg tccgcgccgc cgtaagcgag ctcccaactc
     3121 aaagggactt tggcgatggg ctccgccgga gtaaaacgga gttcccccat catgtcgcgc
     3181 tcgacgcgac gcgccccggt gactatgaat ttgcgtgaca gcgctccgac gcgcagcccc
     3241 gccgtgaagc gcgtggcttt cgcagatggc gcgtacgcat ggcctttgac gatgacatcg
     3301 acgagcggac gtctaagcat cacgtcgatg tcggcctcga gcagctcact cgtcggctga
     3361 agacgcggct cttcgaccaa cgggagctgt tcatctgcga gccgaacgag cccgccgttc
     3421 accgaataag ttcgcttcgc gacgaccccc acgaccgggt gcgggccggc gtagtcgacc
     3481 acgttgaccg ccgaagttcg gcctggactc actggaaaag ccccttcacg ctctgctcca
     3541 gtttcgagac ggggcgctgc accgtatcga agacgatatc actcgactga taattactcg
     3601 gcacgagccg ccacggggca taaggatgcg tccacccgag cggaccgccc aaagcaacgc
     3661 ccatcaagcc ttccaagatc ctagttacgc cggcggccgc ttgcggcccg agtgcccgga
     3721 tcacgaaggg gctgcgcgcc atgcgcatcc cgatgatcac cacaatcaac gtcgcgcgta
     3781 gcccgagcga cacgacgaag cgctccgcga gcttgcccaa gcctttaacg agcccacccg
     3841 caatctttcc gaggatgaac gtgatgagcc cgtcaaccag catcgcggcc aggctcgcga
     3901 tgatatccgc catcgtgagg ccggcctgta ccgtgtagtt cagaatcgaa atgttcttac
     3961 tactcggcag cggaaacggc tccgaacaat cgagttgggt gccgactacg aagctttctc
     4021 cgaccgacac tggacccgtg ggcagacgca cggaggccac cccgaactcc accttgttgg
     4081 cggatcccaa gatgatcacg ggaagcaggg cgtgcagggg catcgtgagg cagatatgag
     4141 caaccacgaa cgccgcgtcg tggcctctgc ccaccaacgc gccaatcggg gaaagtacat
     4201 tcttcgctcc atttcccgtg ttggccactt tggaggaacc gggcgcgcaa gatagccctt
     4261 ctaaccaaac gatcagatgc ggctgtggta ccggaggagc cggcggcggg gggatgggtg
     4321 gcaacgtgaa gtgcagatcg actccgagaa tcgggatcca ttgcgcgatg cacggcttac
     4381 cacccgggaa cgggaacatt aagacgactc ctgagagagt aaagtcagct cagcacgccg
     4441 tggctcttca cgtacgaagc catactgaaa gcgccctcga taagtgagca ccaagcggcg
     4501 ctcggaaacc caccaaaata ctccgtccag acccggcaat aggctgccct gattttctcc
     4561 gacggagata tcgagtttgg gttgcacgcg agggatactc aagcgactag cggcattcgc
     4621 ggctccctcc agcacgatca cgtctcctgg ctcgcactga ttgagcacca gtgaaggatg
     4681 ggcatggttg aagaggctag gccggatgcc ctccacttct cccgcatcca agttcacttc
     4741 cactccctcg cgcgctcgca gaccccaaac catcgggtag ggcccaaacc cagccacgac
     4801 gccaacatcg ttccaccggc tgacgaatgg accttctgca ggttcgatgt tcggcaatgg
     4861 cttaccgagg ctgtcgcgtg catcccggag cagagcgccc ttgccgtgag ggttgtcggg
     4921 aaacgcaacg agcccgagtt cttcctgccg gagcccaccg taggcgttcg cgtacgtcaa
     4981 gggcatgttg gtgaacggtt cgggcgcgga gggcacaggg tctccggtgg ttccgacttg
     5041 ccaatgtcga ttgcccgtta cgcgaagccc gacccgccgc tcgcgcgtgg tcagccacac
     5101 catggtctgc gtggtcgggc gcgagcactg caccgtcgca aacacacaaa catcgacgcc
     5161 tcgcttcttg ggatacatgt ccccgtgcag aacgccgtgc ggagtctcca agaactccgc
     5221 ctgcagcggc atctgttcgc tggctatcgc ggtgcgacca tccgcgccaa tttcgaaagt
     5281 cagcttgacg atagccgccg cgtacacgag cgggtccgga ggaaagctgc ttttgagaag
     5341 gcggatctgc gccggcgtcg agtttgctat ctgcacccgg agccctcacc ttcgctatcc
     5401 gttaccggtg ccgccggttg gtcgcccaga aatcgcacgg aattgcccgg cgaaatctgt
     5461 agagttcccg cgagccacgg cggcaccggc gccggaggat cgcagttcaa cagcacgcgt
     5521 tcgccgagta gctgcacgtc gtcgttggca cgcacttcta cactgccacg ggtcgcgtcc
     5581 acttggatgc gctcggcggt ttcggtcaga gtgagcgcgg tttcgcggag ttctcctccg
     5641 ctgctcagct cgatgcgttc actggctttg acggagaacg tccggcactc ggccgcgata
     5701 tcctgcgctt ggatcgcgat cgccgtcgcg cgcacgcgca cggtggtgcc ggcctcggag
     5761 gtgacgagct cgagctcgag ctcggccgcg cccgaggggg cgcgcacgca caggtgggca
     5821 ccttctgggg ttgcgctgag cgtcgcaacg cggctgtgtc caatcgggat ctcctcggat
     5881 gcagtgggca cgctccgggc gagctgctcc accagctgtc gctcgggctg tcttttagtg
     5941 tctttgagca tcgttgaact ctcgtctgga gacgcctcag gtattcagat tgacggccgt
     6001 gcccgtgacg gtggctcccc cgctgtccag atccaccttg ctgccgcctc ccgcaatgtt
     6061 gactttcgta ctgccgacca cggtgacctc tttgaaaccc gtgatgttgg ccttctgcgt
     6121 gcccgagatt tctatggtgc cgtccttggt gagagtgatc gaagcgcctc cgcactgcag
     6181 cgtgagtttg ctgtcagcct tcagtgtgac atcacccccg tctagcttga gctcgcacgt
     6241 ctcgttcttc aggtggacca gggctttctc catcttgagc tgatcggacg cgtgattcag
     6301 ctcgatcttc ccgtccccct ggagcgtgta agcttgggtc acccccgtct tgtagtcacc
     6361 gattacgtcg agcttctcca gcgtggtcac ctgggtgtgg cgttcggcca agtagaagtt
     6421 ctcccgtagc gcgtgagacg tttctttgtc cttcttggtg atgtccaccg tgcgttcgcc
     6481 cgtcacggtt acggactgat tggcggtcac ggcgacgctc tggttcgctc cgacggagat
     6541 gctctggttt gcacccacgg acacgctctg gttgactttg accttcgtga cttggttctt
     6601 ctcggcttgg atgaacagct cttcttgtcc cttcttgtcc tcgaagcgaa tctcgttgaa
     6661 attgctgtcg aggccgcgaa gcgtgctgcg gctcttgatg ccactctgag tcttgttcaa
     6721 gggcaactcg tacggtggct tgttcagctg attgtaaaca cgccccgtga tgatcggacg
     6781 gtccggatcg ccttccagga agtcgacgat cacctcctga ccaatgcgtg ggatgtgtat
     6841 cccgccgaag ccgctgccgg cccagagctg cgagacccga acccagcaag agctatcgcc
     6901 gtcgaacttc cccaaccgat cccaatgaaa ctgaactttg acccgaccat gcgaatccgt
     6961 ccagatctcg ttcgggtcat cccccactac caatgccgtc tgaggaccct cgatcaccgg
     7021 ctttggtgtg atgcgcgcag ggcggaactg acgtttgcta tccagcgcgc gaatgtccac
     7081 tcgataggtc agctcgctat ccgttcccgc cgacgactca tagccgttca cttggagggc
     7141 ataggcggtc tccagcgtga gatactcctt gttctgatcg gcgcgcggaa actcctccag
     7201 cgtaaaaagg tgcccggcgt tgagcgctcg gacgttgccc gctgccgaaa ccgtctcgta
     7261 ctccacatgc tgctcctcca ggcgcatctg gactcgcgac tcaccttgtg agcgctccac
     7321 gaaatcgatc ggatagtcga actgctcgta gttcgagtgc gcgtgctcct gcggtcgcgc
     7381 gatttggggc gtcaactgag ctcgcggctt ctcgaagtcg aagtcacgcc ctacgtacga
     7441 accagagcgg atccgtcgcg agaaggacca ctggtcgaag tgttcggtct cacggcgttg
     7501 cccttgctga gggggcatgt atcgaatgag cttggcatcc ggcgccggtt cgtgagcact
     7561 gtaggagtcc gccaagacca aggtgtgctt gccgttgccg tggcgaaaga agtagtagat
     7621 cccgacgtgc tccaggagcc tgctcacgaa attcaggtcc gactcccgat actgcaccac
     7681 gtagtccagt accggatagt tctcgtggtg ggtgagccga tcatcgaagt cgctgaaccc
     7741 gtgctcgcgg aagatctcct tgacgatgtc cggcacacta cgccgctgga acacccggca
     7801 attggtgcgc ttgctcaata gccagatcca aggtcgcagc accgcgcggt agcgcacgta
     7861 gcgtcccaac ccgcccacca tggcgaactc ggtgacgatc ccgatgaact ggcgttggga
     7921 ctgcccctgg agctccagca atacccccat cggttgaccc aacagcgcac cgaaatcgat
     7981 cttctcgttc tcgctgagca ggtcgacttc atacgagaac ggcctactga gggcctcgct
     8041 gcctcgcaaa gagagcagca gcaaagcacc ttcatccagc acgctcagaa cctcagccac
     8101 ccggaccatt cgcggaccct accacagggt tcgcgccctg ggttcttcag aagtgacgcg
     8161 gcttttggag gaaatggcgc gcggaaccgc gcagaccaag aaccttccaa gcctatacaa
     8221 cgcggcgatc catcgtgcta gacccgatgc gactctctgc gtcgttgcat gatacccgct
     8281 tcatttacgg ccgcgttgct gggggcatca cttgcctgtg ttacttgcgc cgtaagctgc
     8341 aggcggccga cgtcgcacgc gaacgcggtg gaaagcgcac cgaacgcggg agcgcctgcc
     8401 cccctcgaat cggacgaatg tcatttgaac gaggagctcg atgcactgtt tggtgctcgc
     8461 gagaacgccg ctgaactgga ggcgacgttc cgggctaggc tcctgcccac caacgaggcg
     8521 gcccggcgag ggcgtccgct cgcccatcgg gtggtgactc tgatcgaggg tcgtcgcttc
     8581 cgagagcttg cgagcttgat caatccggag cgaggtctct gccttcgcgc ggcgaagggt
     8641 gccgaatgcc gcacgctctc gcgggcagag ctcattgagt gcggttcatc tcctacccag
     8701 gaacgctggg ccgtggacac cgcggctgac gagagacctc tactcacgtg ttctcgagcc
     8761 ttcgaacgca tcttcttcgt acgcggtttc cgcagcccgg acgcggtcac gttcaactgc
     8821 ttcccacctc tcggtcgagc gaacaacggg agcactattc tgcaacagcc tgcgagtgtg
     8881 tacgtcgagc ttcgcttgaa tgaacgcgag aactcgagcg gcggtgacga aggccggttc
     8941 cgcgcacttt ggctagtatt cgacggtccg ccggacgctc tcgagtgggt cgaaataacg
     9001 gccgaatatc cgggtatgca acgtgacgtc cggattcgtg gttcgagcgc accctgtctc
     9061 caccgggatc cgagcgtttg ccacgaggac tacggcactt gaatcgtgaa ttggtcggca
     9121 atgcggccgt tgacgtcttt gaaataggcg tcggcgcgct tggggtcacc gccgacgtgg
     9181 aagcggatga agaacgcacc aaaggtgccg gccccggttt gcaatacgcc gtttctgaac
     9241 caacgatcgc tggtgtagta ggaggcccac caggtatcgt cgccgtgagt ggtcgtgtcg
     9301 aaggggcgaa tgccgacgcc acccagccca gaggtgaata cgaagttgcg acccggcgcg
     9361 agttgaatgg tgtcgaagag gccggtcgcg ccgtgggcgt tggagcggtt accgacgtcc
     9421 gtgagcgcga gggtacgcga gaacgagtgt tcgtgcccag ttgcgacgag cgcaccgcca
     9481 agcatgcatt cacggtacgc gttccaaccg acttcgtcgg tcttggtgcc aacctgcatg
     9541 tcattctgat tcttgtgcca attgcaaaca gaccagagtg attggtcggc ggccagcgaa
     9601 cttcgaagga agtcgacctg ttcgagcgca tccttcgcgc agcgggtagt gctccattcg
     9661 gtcacaccaa cgcaagattc gaccaggtac agtcctcgga agttgcaggt ggctttgacg
     9721 ccgagttcac ccgcgcaatt catctctggc acccgggcgt ggcgcgcggc aatctttgcg
     9781 gcgtagccgc tggtgccacc ccaggctgcc gcgtcatgat tgccaattag cgcaaagtac
     9841 gggtagtcgg cccccaagat gcgggtgatg cgatcgtccc aagcagtcgg gttattggcg
     9901 tagtcgaagt caccgttgtg gacgaccgca gcggcgcctt catttttgat caagttgagt
     9961 acggcgtccg cattggtgtt cgctccttga tcaccgatga aagcaaccaa gagatccggc
    10021 tcggtcgcac cgggcacacc gccaccccca gaagagccgc cgctgccgct ggtgcctgcg
    10081 ctgccgccgg aatttgcgcc gccggtggca gatccgccgg tggcagatcc gccggctcca
    10141 gtggagccac cgctaccgcc gccacccgac gtctcgtacg taatggcgag tgtggggcga
    10201 tacgcgagag tcgcgtgttc tccggtcgcg agcgcgagct ccttgggatt ggtggcgttg
    10261 gccacgatga ggccggcgtt gctgctcggg ttatccaccc aagcttggac gcgggctatc
    10321 ccggccgcgt tcaggatgac ggtttgggtg ctgttggtgg agccggtgat ggtacccagt
    10381 cgggcccctc gatcggtgtt cgccaacgcc cctggagcgc cccaagggct gcccgcgcgg
    10441 gccgagttcc aagtgacctg gctttcgctc cagggccgca atagctcatg gacgtcatac
    10501 acgttgttgc tgcctttggt gactcgcaac gagatgcggg cctcggtaat gacgctgccc
    10561 ggcgggatgg cactgacatc ccagtaaaag agacaagagc tatccagacc gccgcgttcg
    10621 tcaccgtccg ccgtgcacgt ggtggcggcg cccaaattct gatcgggggc agactgctga
    10681 agcatggcgt ccgccgtcgt gaggtatccg ctacccggcg aaacaccgcg ctgaaagctt
    10741 tgcgtgattg cgagcgcttg gcgctgcgac ccgagttcga gcggctcgat ggcattgcac
    10801 gcgatatacc cgctcgcgca cgtggcaatc gccataccgg cacgagtgcg tgtgggcacg
    10861 agacgatagc ttctgcgcca aagtgacttc gtagaagagc gctccctaat catcatatct
    10921 cctatgtgtg aatccacccg ccacggctcg gagtggacgc ccagcgcgac ttggagacag
    10981 gcattcctcg agcccgcatt agagctcgcg ctgggcaacc gcacctcact gagtgtgggt
    11041 ggagttcgag ctcagaattt atgaaaccct cgcgtcgcca cgctgctgta gaaaaacccg
    11101 ttagtgttgg agccgcgcgt aaccgcggct ggcgggaacc gtcacgcttc ccctcagcgg
    11161 tcgaacgcgc tcgtgacgat ctcttgcgcg gcctcgaccc gcgacgcgca cgaggcagcg
    11221 gacggagccg gcaccggtcc acgcaaatcg aggtagctcg cgccgagcag agcgagccca
    11281 aaccaaatcg tgaggcccgc tgccggcgcg cgcgtgcgag ccagacgaag agtgctctcg
    11341 aaagccggat tggatgtggt gtgtgcagtc atgttgggtt ccttcgaggc ccaacctgac
    11401 gccgaggatt gatgctttca aacggcctaa taggaacgtg ctattaacca aaacttctca
    11461 atgtcggtga ccccactcaa caccctgcag ttgt
//
LOCUS       DASTKU010000759         8999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1936085, whole genome shotgun sequence.
ACCESSION   DASTKU010000759 DASTKU010000000
VERSION     DASTKU010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936085"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..71)
                     /locus_tag="VFQ61_26480"
     rRNA            complement(<1..71)
                     /locus_tag="VFQ61_26480"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(864..1508)
                     /locus_tag="VFQ61_26485"
     CDS             complement(864..1508)
                     /locus_tag="VFQ61_26485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958082.1"
                     /translation="MKVFRKSLLARDQGVTEFSAILLRLCDASGALAAALVDSEGETV
                     DYAGRVEPFDVRVAAAECRLLLGGLHENASRLWPAPNSFIIRSGKHSISVHALSEGYA
                     VVLVLPRHSFDVSARALSEASREIAREAGLTVDVPRGSLLWSRVEVRCVSRKTRRPAA
                     IWQNGAFRPVLVLGRLERPIIGGRDRGYLIRLDTGAELLLVREPLGRWFAAAPL"
     gene            complement(1505..2422)
                     /locus_tag="VFQ61_26490"
     CDS             complement(1505..2422)
                     /locus_tag="VFQ61_26490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9958083.1"
                     /translation="MKTTRVLRTLLIGALLCSCKARPPVPTGNGRLASGMLARVADQS
                     IPIEQVSRIAAAQGISERSALDRATADALFAVEARRSIGSPSVRQAERAAAARSLLEE
                     LARHAQSQGTPTDKEIEQLTIDHWIDWDRPPAARVVHVVALLPSNGEARASREVAERV
                     QAAVHGVRAPEVFAERARGVPHQGIELRVEELPFITPDGRGISIKEPRMAAGQFEPAF
                     AAAANALSTEGDQSPIVETRFGFHVLMLLERLPERRLPLEERRRIFEPEVLARRARSE
                     KNTLVQRLGAEAQVGVARDVDAQTETLFR"
     gene            complement(2449..2631)
                     /locus_tag="VFQ61_26495"
     CDS             complement(2449..2631)
                     /locus_tag="VFQ61_26495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958084.1"
                     /translation="MARLADIVSVLLLCGAAACFAFGLSALGEHRDLFALYWLLIGAL
                     TLRAATQLLRPRAGSR"
     gene            complement(2631..3347)
                     /locus_tag="VFQ61_26500"
     CDS             complement(2631..3347)
                     /locus_tag="VFQ61_26500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958085.1"
                     /translation="MARSATSPSAFTGASAQANLLLPGLYAWVTTVLVPASSARATKF
                     AQLAAALSLLSLLAGAAFRTRATRASRGFGIHLFIGTSTACWLLLARAGVPLAPQPFD
                     AALGALGWTLYTFGWGELRERKQVPESDPTVLPGPPLTARHPWPRSTEFVLGIGVLGC
                     FLLAVLAFRVDRPGQAAMAHGLSLLLALGLLGSTVEVALGRRPRELGGSAERFNRAAT
                     PLALLAMALGLSAVFWILER"
     gene            complement(3401..4891)
                     /locus_tag="VFQ61_26505"
     CDS             complement(3401..4891)
                     /locus_tag="VFQ61_26505"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET9958086.1"
                     /translation="MPLSVSLASDPLAEAVDTLVVSAFASEVAAHPTLLALDHALGGA
                     LLAHIKAEEFEAKFEQTLEVPLLAKLVPGKLKARKLVLVGAGAVQELNPARLRSLIAG
                     AIRGCSNAKSLAFVLPGAELDLRVVGEAIGLGSYRFTKYFTGDRRPKRELAKVSVCVP
                     GAKPTAEAKRQLARGVELAQCVAIARDAVNEPPNVMNPEGMAALARKIAQTHKFKIKV
                     LDKKGILAAGMNLHYAVGQGSANEPRFIHLSYVPKRRKKKLVFVGKGLTFDSGGLCIK
                     PAAGMGEMKSDMGGAAAVLGLMAAVGVLRPDVEVHGIVGAAENMPDGEAYRPADVFTS
                     LDGKTVEIVNTDAEGRLVLADALCYAARLEPDVIVDAATLTGAALVALGKTCSAFYTA
                     DDDLAHGLEAAARTAGESFWRMPLIDELAEQLKSDVADLKHTADRWGGSITAALFLRE
                     FVGKVPWIHCDVAGPVLSDRSRGVVPKGGTGHPVLTFVSFVENLAR"
     gene            5040..6002
                     /locus_tag="VFQ61_26510"
     CDS             5040..6002
                     /locus_tag="VFQ61_26510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958087.1"
                     /translation="MFRWLSRTLACCATLGLAGVCWSTAANAQDVPSTGSLPPLPPDG
                     PATPGSPPASTSPPQPGAAPASGNAPPIPAYVTPAPPPPSPGYNRPAYDEPPPPPEEP
                     VPPPPQKPKSDFSVPAFSVRVDPLNWLLDGRLGLELEAQVVKFITVEFVPVFVTSATP
                     PSLRIGDLDGKIEQHSNGLGALSGTSLGAGFWLSGTALRGTVLRAIFTNYGYRYTASD
                     AAGQFDEVKHTERRFYGFIGSHSRFGAFTMAGGFGIGVELNKENRCFESVSPVRASAQ
                     CDKDQQLILVERPRSGAGAQVADLHSWTYPVELMFRFSLGVTID"
     gene            6025..6759
                     /locus_tag="VFQ61_26515"
     CDS             6025..6759
                     /locus_tag="VFQ61_26515"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9958088.1"
                     /translation="MQQTTETRSARTTLVTGATGELGSAVIEYLAGLGGSVIAVDRPR
                     ARDRLAGLAKLGPLVRAVELDTDHAHAWLDRERWQPDGAVPDGAVLTAGAYAGGKRLV
                     DEADDATLARLLESNLTSAYRALSALLKGMLPAGRGSIVLIGSKAAVRPWESVGSGAY
                     AASKAGLLALVQSAAAETIGSGVRINAVLPSTIDTAPNRKAMPAADPSAWVSKQSLCD
                     VIGFLLSDASRDVSGALIPVYGSRSV"
     gene            complement(6763..7494)
                     /locus_tag="VFQ61_26520"
     CDS             complement(6763..7494)
                     /locus_tag="VFQ61_26520"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HET9958089.1"
                     /translation="MEGIVRARRDWANGLMSLTVEAALPTFSPGQFVNISLSRGGNTV
                     RRAYSFASAPGELAEFYVSEVPGGALTPTLFGLQVGDSLSIDGRPQGFFTLEWVPPAE
                     VLWMLATGTGLAPFLSMLRSGQAQARFRRIVLVHSARHVQELSYAEQLAQLAQNSSCH
                     ITVLRTVTREACEVPILSGRITSLFNSGELEARAGTALLPTGHVMLCGNPGMIDEMTA
                     LLATRGLRRHRVRSPGHITSERYWD"
     gene            8217..>8999
                     /locus_tag="VFQ61_26525"
     CDS             8217..>8999
                     /locus_tag="VFQ61_26525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958090.1"
                     /translation="MAAPLPPSFAPAAEVSSAKQAEKPALDSWEPPPEAVAAAGLTPS
                     SSTAASAEPIRNSQPEPVQPQMPAPPPNELPHPAPGDMSVSSPSPGPAAAAASTKEAQ
                     SKAPSQPAAVRENKLSFGTDLNRNKGQTGAQPGQKLKASLAAQASSKPKTTELKGVEK
                     VDPKAPAAAEHKPLGAERSPFEPRAAAIKAAGPTPAEHKTTTLGLGFDPKLHPAPVET
                     KAAEPPAPIKTEPLLFAVDSPGGDRELSETQIAEEITKGSISE"
BASE COUNT         1698 a         2970 c         2780 g         1551 t
ORIGIN      
        1 tctcgcacga cttgcatgtg ttaggcgcgc cgctaacgtt cgttctgagc caggatcaaa
       61 ctctccagtt tgaatccttc ggctagcgtc ggaggctaag tccagacgcc gccattatgc
      121 tcgctctctt cgcactcgtc gtcttcgggg ggcgaagctc gacggtgctt ctcacgcgat
      181 ttctcgcgta caatcagact cagttttcaa ggaccgaggg ctgaagccgt tttgacttcg
      241 ttgccgttgg ggaggcgcgt tctagttgat gtcgcggtct ctgtcaaccg cttttttttc
      301 aacttcgttc gcgacttccg tcacttggtc gcttcggctt tcgcctctgc ttcctcgttt
      361 tcggagtcgc gctctttagt tgctgcggcg gttttcgtca accactctct caactttttt
      421 catcgtgcct cgctagcggc gattgcacga tgtttgcgcc ccctgagctc tttgccgggc
      481 ggcttcttat ggggaagcca tcggcgttcc gagccggtct tgaaaggtag accctttcag
      541 aaaaacggca aggagagatc aggtgccgca caccgggaac tccggtgccc tgcggcgtct
      601 cggctggtgt cgccgagggg ggcgatacat ggaccgttcc tgagactcga gcaagatctt
      661 tttttgcgcc ttccaggatc tggctgaaat tccgctgtgc gctggtgtgc gttcgaccga
      721 cggaccgatc ttgctcaggc agatgtcgcc gacgagtggg gctttggacg ccctctggag
      781 gcctgattgg ggtgatcagg cccggtgcag ccgtcaggtg gctaccgccg gcttacgctg
      841 ggcgttcgag tggttcctcg gagctacagc ggtgccgcgg cgaaccatct tcccaagggc
      901 tctcgcacca ggagtagctc tgcccccgtg tcgagccgga tcaggtagcc gcggtcccgg
      961 cctccaatga tcggacgttc gaggcgcccc agaacgagga ccgggcgaaa cgcgccgttt
     1021 tgccaaatgg ctgctggccg gcgggtcttc cgagagacgc agcgcacctc cacgcggctc
     1081 cacagcaggc tcccgcgggg aacgtcgacg gtcagcccgg cttccctcgc gatttctcgg
     1141 ctcgcctcgc tcaacgcgcg agctgaaacg tcgaagctgt gacgcggcag caccagcacc
     1201 accgcgtaac cctcgctcag cgcgtgaacg gatatgctgt gtttgccaga gcgaatgatg
     1261 aagctgttcg gagcgggcca gaggcgggat gcgttctcat gaagccctcc tagtaagagc
     1321 cggcactccg cagccgccac gcgcacatcg aagggctcga ccctccccgc atagtcgacc
     1381 gtttcaccct cagaatccac taatgctgcc gccaaggcgc cgctcgcgtc gcacagtcta
     1441 agcaggatgg cgctgaactc ggtcaccccc tgatcgcgtg ccagcagcga cttgcgaaac
     1501 actttcatcg gaatagcgtc tcggtttggg cgtcgacgtc ccgcgcaaca ccgacttgcg
     1561 cctcggcgcc caagcgctga acgagggtgt tcttttccga gcgtgccctt cgcgccagaa
     1621 cctcgggttc gaatattcgg cgccgctctt ccaacgggag ccgccgctcc ggcaatcgct
     1681 ccaacagcat caagacgtga aagccgaagc gggtttctac aatcggactc tggtcgccct
     1741 cggtactgag ggcattcgcc gccgccgcaa acgcgggctc gaactgcccc gccgccatgc
     1801 gaggctcctt gatcgagatg ccacgaccgt cgggagtgat gaacggcaat tcttccacgc
     1861 gcagctcgat gccttggtgg ggaacgccac gcgccctctc cgcgaagacc tcgggggcgc
     1921 gcacgccatg cacagcggcc tgaactctct ccgcaacctc gcgcgatgcg cgtgcctcgc
     1981 cgttgctggg aagcaaagcc accacgtgca ctacgcgagc cgccgggggg cgatcccaat
     2041 caatccagtg gtcaatcgtg agctgctcga tttccttgtc ggtcggcgtg ccctgggact
     2101 gcgcatgccg cgcaagctcc tcgagcaagg atcgagcagc ggcggctcgc tcagcctggc
     2161 gaacactcgg gctcccgatc gaacgacgcg cctccaccgc aaacagggcg tccgcagtgg
     2221 cccgatcgag ggcgcttcgc tctgatatcc cttgcgcggc ggcgatgcga gagacctgct
     2281 cgatcgggat gctctggtcg gcgacccgcg ccagcatgcc gctggcgagc cgcccgttgc
     2341 cggtcggaac gggagggcgt gccttgcagc tgcaaagcag ggctccgatc agcaacgtcc
     2401 gcagaactcg cgtggtcttc acggcaaccc tacttcaaca cgggtcgatc accgtgatcc
     2461 agctctcggt cgcaacagtt gagtcgcggc acgcagagtg agggcgccaa ttaggagcca
     2521 atatagggca aacaggtccc ggtgctcccc gagcgccgac agcccaaacg caaaacaggc
     2581 cgctgccccg cacaacaaca acacactcac gatgtccgca aggcgcgcca tcaccgctcc
     2641 aggatccaga acaccgctga caatcccaat gccatggcca aaagtgcgag gggggtcgcg
     2701 gcgcgattga aacgctcggc gctgccgccc agctcacggg gtcgccggcc gagggccact
     2761 tcgacggtac tgccaagcaa gccaagcgcg agtaagaggg acagcccgtg ggccatcgcg
     2821 gcctgaccgg gccggtccac ccgaaaggcc agcacggcaa gcagaaagca gcccaagaca
     2881 ccaatgccca aaacgaactc cgtgctgcgc ggccaagggt gacgcgctgt cagcggtggg
     2941 ccgggtaaaa cagttggatc gctctccggc acttgcttgc gttcccggag ctcgccccac
     3001 ccgaacgtat aaagggtcca ccccagcgcg ccgagagcgg cgtcgaacgg ttgaggagcg
     3061 agcggcaccc ctgcccgagc caagagtagc cagcacgcgg tgctggtccc gatgaataga
     3121 tgaatgccga atccccggct cgctcgggtc gcccgcgtcc tgaatgcagc ccccgcgagg
     3181 agtgacagta acgagagagc ggccgcaagc tgggcgaact tcgttgcgcg tgcactgctc
     3241 gcgggtacga gcaccgtcgt cacccaagca tacagaccgg gcagcagcag attggcctgc
     3301 gccgaagcgc ccgtgaacgc ggaaggggac gtagcgcttc gggccatctc tcagctaagc
     3361 cgaaggcccc ctccaactgc agcgcacgcg cgcggatcag tcaccgcgcg aggttctcca
     3421 cgaagctaac gaaggtcagc acggggtggc ccgtaccgcc tttgggcacc acgccacgtg
     3481 agcgatcgct cagtacgggg cccgcaacgt cacagtggat ccagggcacc ttgccgacga
     3541 attcgcggag gaatagagcg gccgtgatcg aacctcccca gcgatccgcc gtgtgcttca
     3601 gatccgcgac gtcactcttc agctgctccg cgagttcatc gatcaacggc atccgccaga
     3661 agctctcccc agccgttcgt gccgcagctt ctaggccatg ggcgaggtcg tcgtcggccg
     3721 tgtaaaaagc ggagcacgtt ttgccgagcg ccactaacgc ggcgcccgtc agcgtggccg
     3781 cgtccacaat cacgtccggc tcgagccggg ccgcgtagca gagtgcgtcc gctagaacca
     3841 agcgaccctc cgcgtccgtg ttcacgatct ctaccgtttt tccgtccaag gacgtgaaca
     3901 cgtcggcagg acggtaagct tcaccgtcgg gcatgttttc ggcagcaccg acgatgccgt
     3961 gcacttcgac gtccggacgt aacacgccca cggccgccat caagccgagc accgcggccg
     4021 caccgcccat atccgacttc atttcaccca ttccggcggc gggcttgatg cacaatccac
     4081 cagaatcgaa agtgagcccc ttacccacga acacgagctt tttcttacgc cgtttgggga
     4141 cgtaggagag gtggatgaac cgaggctcgt tcgcgctgcc ctggccgact gcgtagtgca
     4201 gattcatgcc cgccgcgagg atgcccttct tgtccaagac tttgatcttg aacttgtggg
     4261 tttgcgcgat cttgcgtgcc agggcagcca tcccctccgg gttcatcacg ttcggcggct
     4321 cgttgaccgc gtcccgcgcg attgccacgc actgcgcgag ctcgacgccg cgcgcgagct
     4381 gacgcttggc ttctgcggtt ggcttggcgc cgggcacgca gacggagacc ttggccagtt
     4441 cgcgctttgg ccgacggtcg ccggtaaagt acttggtgaa gcgatacgag ccgaggccga
     4501 tcgcctcacc gacgacgcgc aagtcgagct cggccccggg gagcacgaag gccaaactct
     4561 tcgcgttgga gcagccccgg atcgctcccg caatcaacga acgcaagcgc gccgggttca
     4621 gctcctggac agcacccgca ccaaccaaca ccagctttcg agccttgagt ttgcctggaa
     4681 cgagcttcgc taagaggggt acctccagcg tctgctcgaa cttggcttcg aattcttctg
     4741 ccttgatgtg ggccagcagc gccccgccga gagcgtggtc caacgccaac aaggtgggat
     4801 gggcagcaac ctccgaggca aacgcgctta cgaccagcgt gtcgacggct tccgcaaggg
     4861 ggtcagacgc aagagacact gataacggca tcgacacaca gacctagtcg tctctgagct
     4921 accgagcaat ccccgccgct caaagaacca ccggccgcag tgacccggcc acagcgacca
     4981 gcgcgagttc ttgggctgcc gggtcgcgta gcggcccggg ctccagtatc ctggtgcgca
     5041 tgttccggtg gttgagtcgc accttggcct gctgtgcaac gctcgggctg gccggcgtct
     5101 gctggtcgac cgcggcgaat gctcaggatg tgccttcgac gggatcgctg cctccactgc
     5161 ctcccgacgg acccgccaca ccgggatcgc cgccggcttc tacgtcgcct ccccagcctg
     5221 gggccgctcc cgcaagcggg aatgcgccgc ccattcctgc gtacgtcacc ccggcgcccc
     5281 ctccgccctc gcctggttac aataggcctg cgtacgacga gccccctccg ccacccgagg
     5341 agcccgtccc tcccccaccc cagaagccca aatcggattt ctcggtgccc gctttctccg
     5401 tgcgagtgga tccgttgaat tggttgctgg acggcagact ggggttggag ctcgaggcac
     5461 aggtagtgaa gttcatcacc gtcgagttcg tgccggtatt cgtgacgagc gcgaccccac
     5521 catcgctccg cattggagac ctggacggga agatcgaaca gcactcgaac ggcctaggcg
     5581 cgctctcggg cacctcgctt ggggcaggct tttggctcag cggcaccgcg ctacggggca
     5641 cggtgctgag agccattttc acgaactacg gatatcgcta cacggcttcc gacgccgctg
     5701 gccaattcga cgaggtcaag cacaccgagc ggcggttcta cggtttcatc ggttcgcatt
     5761 cgcgcttcgg tgctttcacg atggccggcg gattcggaat cggggtcgag ctcaacaagg
     5821 aaaatcgctg cttcgagtcg gtcagtccgg tccgtgccag cgctcagtgc gacaaggatc
     5881 aacagctgat tctggtcgag cgcccgagga gcggggccgg agcgcaagta gcagacctac
     5941 actcgtggac ttatcccgtg gaattgatgt ttcgattctc gctcggcgtg acgatcgact
     6001 gaagccgcaa ggccaggaat gaacatgcag cagacaaccg agacgcgatc tgccaggact
     6061 acgctggtaa caggcgccac cggcgagctc ggctcggccg tgatcgagta cttggcgggt
     6121 ttgggcggat ccgtgattgc ggtagatcga ccccgcgcga gggaccggct cgccgggctc
     6181 gcgaaattgg ggccgctcgt cagagcggtg gagctcgaca cggaccatgc ccacgcgtgg
     6241 ctcgatcggg aacgctggca gcccgacgga gccgtgccgg atggagccgt gttgaccgcc
     6301 ggagcctacg caggcggaaa gagactggtg gatgaagccg atgacgcgac gttggcgcgc
     6361 ctattggaat ctaacctgac gagcgcgtat cgagcgctct cggcgctcct caaaggcatg
     6421 ctccccgcgg ggcgaggcag catcgtcttg atcggctcga aagcagccgt tcgcccatgg
     6481 gagagtgtag gttcgggcgc ttatgcggcg agcaaagcag gcttgttggc tttggtccaa
     6541 agcgcggccg cggagacgat tggaagcgga gtacggatca acgccgttct tccgagcacg
     6601 atcgatacgg ccccgaaccg caaggcgatg cccgccgcag atccgtcggc ttgggtttcg
     6661 aagcagtcgc tgtgcgatgt aatcggcttt ctgctctcgg atgcgtcacg cgacgtttca
     6721 ggggcgctaa tcccggttta cgggagccgc tcggtgtgag cgtcagtccc aataacgttc
     6781 agaggtaatg tgaccagggc tgcggacccg gtgccgtcgc aacccccgcg tggcgagcag
     6841 cgccgtcatc tcgtcaatca tgccgggatt gccgcacaac atgacgtgcc ccgtgggcaa
     6901 cagggccgtg cccgccctcg cctccaactc tccagagtta aataaggacg tgattcgacc
     6961 gctcaaaatc gggacctcgc aggcctcgcg cgtcaccgtg cgtaacaccg tgatgtgaca
     7021 cgacgagttc tgggcgagct gcgcgagctg ctcggcgtaa gagagctctt ggacatgccg
     7081 tgcggagtgc accagtacga tgcgccggaa tcgagcttgc gcttgcccgg agcgcagcat
     7141 ggacaggaac ggcgctagtc cggtgccggt cgccagcatc caaagtacct ctgctggcgg
     7201 aacccactcc aaagtgaaga agccctgcgg ccttccgtcg atggagagcg aatcgcccac
     7261 ttgaagcccg aacagcgttg gcgtcagcgc cccaccggga acttctgaaa cataaaactc
     7321 cgccaattca cccggcgcgg acgcgaatga gtaggcgcgc cgaaccgtgt tgccccctcg
     7381 ggacaaggaa atattcacga actggccggg tgagaatgtg ggcaaagcgg cttccaccgt
     7441 gaggctcatc aagccgttgg cccaatcgcg gcgcgcgcga actattcctt ccatgaagct
     7501 cacgggtgct ggcgcgcaaa gcctacctcg aacgcgggcc ccgcgagcag agggtttcag
     7561 cattcaattg cgaggcacgg tgacactcaa caggcattca ggacggccgg ttcactacct
     7621 gcggcagcag caggctcgtg ccccgtaggc gcgccagtgc gacacgcgtc ttggagcgac
     7681 tgctgcctaa ggacgacata gctcgcacga gcaccggaaa actgagtctt ttgcctgcag
     7741 agcgtgtaac ctaccaactt aggatgccat ttcccgtcac gtgcccggcg tgtggaaaga
     7801 gcttctcgat ctcggaccaa gtcttcgatc agaagattcg cgggcgcatc gtttccatca
     7861 agtgcaaaca gtgtcagtct gcgatccgag tcgatggcag agtgaatccc ggcgcagatg
     7921 ccgttgatcc acacaagccc ggcgacgtcc gagcagctga cgcttcgcaa ccgaatgctt
     7981 ccgctgcgag caccgtcggt tcgactcctc ccgcggacag gcctgcctcg tcacggcccc
     8041 aagccgcgcc gctcgcaccg ggcaatgccg aggtgacggg agctggagct ctggttgaag
     8101 gctccgcacc gatcgaaggc gcgaggccgg gaccggcgtc gacagcgccg tcaacgtccg
     8161 gttcgacttc accagaggcg gtgcctaccg tcgcggccac tctggagtcg aatcccttgg
     8221 cggcgccgct accaccaagc ttcgctccgg ccgcagaggt ctcttcggcg aagcaggctg
     8281 agaaaccagc tctggactcg tgggaaccgc cacccgaagc ggtggctgct gcaggcctga
     8341 ccccttcgtc gagcacggcc gcttccgcag agccaatccg gaactctcag cccgagccgg
     8401 tgcaacctca aatgccggcg ccgccgccga acgagctgcc gcacccagcg ccgggagaca
     8461 tgtccgtttc ctcgccttcc cccggtccag ccgccgcggc cgcgtcgacc aaggaagctc
     8521 agtcgaaagc accgagtcag cctgccgccg ttcgcgaaaa caagctgagc ttcggtacgg
     8581 atctcaatcg gaacaaaggc cagaccggcg cacaaccagg ccaaaaactg aaagcgagct
     8641 tggccgcaca agcgagctcg aagcccaaaa ccaccgagct gaagggcgtt gaaaaggtag
     8701 acccgaaagc gccagcggcc gctgagcaca agccgctcgg ggccgaacgg agcccgttcg
     8761 agcccagggc cgcagcaatc aaagcggccg ggcccacccc ggctgagcac aagaccacga
     8821 cgctcggctt gggtttcgat cccaagttgc accctgcgcc cgtggaaacc aaggccgctg
     8881 aaccgcccgc ccccatcaag accgaaccgt tgttgttcgc ggtggattca ccgggaggcg
     8941 atcgggagct gagcgaaact cagattgccg aagagattac gaagggcagc atcagcgag
//
LOCUS       DASTKU010000760         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1936621, whole genome shotgun sequence.
ACCESSION   DASTKU010000760 DASTKU010000000
VERSION     DASTKU010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936621"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VFQ61_26530"
     CDS             <1..386
                     /locus_tag="VFQ61_26530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958091.1"
                     /translation="YMLFGTTTDAKPVTLPSPEDTSNSSEAELFTALDMHTECYSYFL
                     LDRFIQRQLGAKLVKKISIRFLLQLDMCHRCRQAATLFETSYPSESWSSSAQSKEAVP
                     STLVDDVSHLLAQHPKLQRWDPFEC"
     gene            complement(436..1836)
                     /locus_tag="VFQ61_26535"
     CDS             complement(436..1836)
                     /locus_tag="VFQ61_26535"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HET9958092.1"
                     /translation="MKTMMSHRRGSARAMAVMALVAAACSGEADPSASAQADSNVRRK
                     DASAESSGALSGSRASDSSPGVPDGEGSRKEWTLAIYLGSETLSDQAAIANLNALEQR
                     YAELSGFMDIIVLADGYFESLEAGWDDRTRILKIRSDDSVRIVSEIAPPPETKIAKLL
                     EAGHGELALSTPEVLQAFLAFTQAHYPARHFALNVVDHGDGWRGAVFDYDEEMRMFGP
                     LRNKQYHDVFATLAQPIDVLGFDACIMQEASANTWWQLGGSKVKFSVASEQVQGVLGW
                     NMDGVARRLAERRRGDGQLSPRAFATEVVNGFYDVPEGWNDTLSALDLSLWPQVLEQL
                     DALGAAMSAAGGMANPIIKSVVDERVPRNYGAMVGLNAAYSEEDLVDGIELCLALEQA
                     FEATTAVNQMARALRDAIEKAVVVNVSNSSTSHGVTLHLPRTGIGVEGSQSEFEKHAA
                     EVLDLTPNWRAFLRSI"
     gene            complement(2019..2309)
                     /locus_tag="VFQ61_26540"
     CDS             complement(2019..2309)
                     /locus_tag="VFQ61_26540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958093.1"
                     /translation="MLEPTFTGGPRASPRGPYTNAEPGVGRVSERPTIVGLESASDRN
                     DHLTAAPIPTGQTESEVLWFVNLELEDETAWEGVRDGINRSTERRSTDSLTS"
BASE COUNT          488 a          904 c          737 g          486 t
ORIGIN      
        1 cgtacatgct ttttgggacc actaccgacg ccaaaccggt cacacttccc tcacccgaag
       61 acacaagcaa ctcctccgag gccgagctat tcactgccct ggacatgcac accgagtgct
      121 actcatactt cctgctggac cgattcatcc agcgacagct cggagccaaa ctagtcaaga
      181 aaatctcgat tcgattcttg cttcagctcg atatgtgcca ccgttgccgc caggccgcga
      241 cgctgttcga gaccagctat cccagcgagt cgtggtctag ctccgctcag tccaaagaag
      301 cagtaccgag taccctcgtg gacgacgtca gccacctgct cgcccaacac ccgaaactac
      361 aacgctggga tcctttcgag tgttgacccg cacacgccgg gaagtacgct ccctcgaacc
      421 cgcgccgtgc cccaactaga tggaccgcag gaaggccctc cagtttggcg tgagatcgag
      481 gacttcggcc gcatgctttt cgaactcact ctgactccct tcgacgccga taccggttcg
      541 cggcaggtgc aaggtgacgc catggctggt cgacgagttc gagacgttga ccaccacggc
      601 tttttcgatc gcgtcgcgca atgcgcgagc catctggttt accgcagtcg tcgcctcgaa
      661 cgcttgctcg agggcgagac aaagctcgat tccgtccacc aaatcctcct cgctatatgc
      721 ggcgttcaac ccgaccatgg ccccgtagtt gcgcggcacc cgctcgtcca cgacggactt
      781 gatgattggg ttcgccatcc cgccggccgc gctcatggct gctccaagcg cgtcgagctg
      841 ctcgagaacc tgtggccaaa ggctgaggtc gagggccgag agggtgtcgt tccagccttc
      901 gggcacgtcg tagaagccat tgaccacctc cgtcgcaaag gcccggggtg agagctgccc
      961 atcgccgcga cggcgctcgg ccaaacgccg tgcgacgccg tccatattcc acccgagcac
     1021 gccctgcact tgttcactcg ctacggagaa cttgaccttc gaaccgccaa gttgccacca
     1081 cgtgttcgcg gacgcctcct gcatgataca ggcgtcgaac ccgagcacat cgatcggctg
     1141 cgccagcgtc gcgaacacgt cgtgatattg cttgttacgc agcggcccaa acatgcgcat
     1201 ctcttcgtcg taatcgaata cggctcctct ccatccatca ccgtgatcca cgacattgag
     1261 cgcgaaatgg cgcgctgggt aatgggcctg cgtgaacgcc aggaacgcct ggagaacctc
     1321 gggcgtcgac aaggccagtt cgccgtggcc ggcttcgagt agcttagcga tcttcgtctc
     1381 gggcggaggc gcaatctccg aaacgatccg gaccgaatcg tcggacctga tcttcaaaat
     1441 gcgggtgcgg tcgtcccagc ctgcctcgag gctctcgaag tagccatcgg cgagaacgat
     1501 gatgtccatg aaccccgaga gctcggcgta gcgctgctcc agcgcattga gattcgcgat
     1561 tgcggcttgg tcgctcaacg tctccgaacc caggtagatg gccagcgtcc attccttccg
     1621 ggagccctcc ccgtcgggca cgcctgggct cgagtcactt gctctcgatc cggaaagagc
     1681 cccactgctc tccgccgacg catccttgcg gcgcacgttg gaatctgcct gagcagacgc
     1741 cgacgggtcg gcctcgccac tacaagccgc agccaccagt gccatcaccg ccatcgcgcg
     1801 cgccgaacct cttcggtggc tcatcattgt cttcatggaa tcctcgcgat ctcatcgaga
     1861 gccgcgacaa gccgccaccc tgacagcctg acagcaaccc atctctcttc attcaccgtg
     1921 ttggattcgc gaaaaacggg tcaccccggc ggcaaatccg cgcgcggtcg ggctcgctta
     1981 caccacgacc gcgcgcgccg gccccctcta ggcgccgctc acgaagtgag cgagtccgtc
     2041 gatcgccgct cggtagatcg attgatcccg tcccgaacgc cttcccaagc cgtctcgtct
     2101 tcgagctcga ggtttacgaa ccacaacacc tcggactctg tctgcccggt ggggatcggc
     2161 gcggccgtga ggtggtcgtt ccggtccgag gcggattcga ggccgacgat agtcgggcgc
     2221 tcggagacgc gacctacgcc gggctcggca ttggtgtacg gcccacgggg ggatgcgcgc
     2281 gggccgcccg tgaaggtcgg ctcgagcaag ttctgcctgg ctatcgcttt cagccgctcg
     2341 atgtctacgc cctgatcccc aaagggcgaa tccgcgttcc acgcatcgca gcgagcctcg
     2401 atgtgctgcg tgaggctcta ctcgaactgg cttagcatcg acggcgccga gcgacctcgc
     2461 gcgaatcgtt cgctcgtggt gtcatggcgc gtcgttcacg agagaagacg aaacccaaaa
     2521 ccatgcgaaa ctgggcgtct cgcatgaccg tcgccgtccc cgctctggag agccccaatc
     2581 ccgccagcga tccgcccgcc ccgaactttg cgagc
//
LOCUS       DASTKU010000761         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_371894, whole genome shotgun sequence.
ACCESSION   DASTKU010000761 DASTKU010000000
VERSION     DASTKU010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371894"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFQ61_26545"
     CDS             complement(<1..556)
                     /locus_tag="VFQ61_26545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958094.1"
                     /translation="MSLASYVLMGITALPTFHEDVGDRYSEQKRIQAETIATAIAEVA
                     EKAQGWPGTPRELASLLLTVAWHETRLSLRIHEGNCRPFECDHGRARGLWQLHMHASL
                     PRERWLTVAGLDPDSTRAAAQEAARALIRSRRMCMSVSRGADWVPYTLVAFAGRGCRG
                     QLPDIQRRVRTFHHLVAIEPKKNGS"
     gene            complement(1765..3246)
                     /locus_tag="VFQ61_26550"
     CDS             complement(1765..3246)
                     /locus_tag="VFQ61_26550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958095.1"
                     /translation="MSNPQTLQKLRALLDRVSRRAQEPRDGRASELSSRAADKSRAAE
                     PKRTDVQVGTIASPAFTPGVATVPAREPIPPPRALSEPPPGLAARARTSPPVAAPPAS
                     VPAAPVAAVPVPAMPVAAAPPPVVPVVTSAGRAPDPSSLSDIFWDDGGPGSAPKASTP
                     PAVAPIPPGTTPRPSTPAPNEVAARADIPAAPESRERPAGSSDALWEAAHGSLTPASQ
                     KPEPVAAAPEVTIEATEQLTEDDFLEMSSDVLESVPPSGEVSEELPLPEGARWVTQAP
                     PADLHEEQPPSSSHRPKGPGTLDEAVAQAEAEAVAIQEGREVPLKTPPPESGPQEAPI
                     PAGLAASAPVFDELLEPAPLAPPFPGLDAGPTPEQVGETIEFPEASRVSLELDAPLAS
                     GYPAHDDGEAVAQIGIEQRGLEQGRLEDILDVPEPPPDERQSGEIPAAAAAYAAEPPE
                     KETLIRPHVAHAVVSQHFRAPEQPTDPRTMLAWLDASIGLMSD"
     gene            complement(3243..>3442)
                     /locus_tag="VFQ61_26555"
     CDS             complement(3243..>3442)
                     /locus_tag="VFQ61_26555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958096.1"
                     /translation="VLEGAIQRGFGRDVSVVCLGRDDNVERFLVTGAQAAAHARAALD
                     SGASFGQVLVELQRGSGRVAS"
BASE COUNT          616 a         1081 c         1075 g          670 t
ORIGIN      
        1 cgctaccatt tttcttgggt tcgatcgcga ccagatgatg aaatgtgcgg actcgacgct
       61 ggatatccgg caactgcccg cgacagccgc ggcctgcaaa agccaccaag gtataaggca
      121 cccaatctgc gccgcgggaa acggacatac acattcggcg gctccggatc agggctcgcg
      181 ctgcttcctg ggccgcggct cgggtggagt ccggatcgag gcccgcgacc gtcaaccagc
      241 gttcgcgcgg tagtgaagcg tgcatgtgta gttgccagag cccgcgggcg cgtccgtgat
      301 cgcactcgaa gggtctgcaa tttccctcgt gaatgcggag cgaaaggcgt gtttcatgcc
      361 aagccaccgt aagtagaaga cttgccaact cccttggggt acccggccaa ccctgcgcct
      421 tttccgccac ctccgcgatt gcggtcgcga tggtttcggc ttggatcctc ttctgctcgg
      481 aatagcggtc acccacgtcc tcatggaacg ttggcagggc cgttatcccc atcaaaacat
      541 agcttgcgag actcacgaca tcagcgcttt caacgcagac aacaaactac cactgccagc
      601 aaactgctat ttgcccgcaa actaccacag cggccagaac cacttctacc gccttggtgt
      661 ttgcctaacc gtgtcttacc attctcgtgg cgaatacaag agtggaactc tgcaccagat
      721 tgagtgtctg gaccgaatgc ctagtcactg gcaatctcga agtattggat atactgggaa
      781 atacccgtgg gccggccagg aggtcagtgt tggcaacttt tttctggtga acgcttttgg
      841 cgcgaatcac aaataagtga gcatggattt cagcgcgtga aagcgcggtt caatcactcc
      901 aacacatggt gaatctctgt gcgtacgcgc tgtcgattcg ccgttgtgtg agccgatcac
      961 accgcctgtg cagttcaacg cgcaatctgc tttgtgaatt ggccaatgtt cgatcggccg
     1021 tgtgaattcg gccgtacgca ttcggccgcc agtgcccctc aacgcgcaaa atcgagtgcg
     1081 ccgcagggct ttggcgtatg actaagctta tttttcagac cctgttccgt tgtggcacgg
     1141 cagtggcgcg ccgctctgat cgcgttctga cgagctccgt tgctggtgcg gtcgtgcaat
     1201 tggcgtttca gctgcgcgcg cgtgcgtggt gtgcacggcg agaacggccc acgccatgcc
     1261 ctgtgcgtgg cattgtctcc aggtccgact ggacgggccc tttacggtag tgcgacagcc
     1321 gcgtcctgac gtgaatcggc gccgacgtgc ttgctattag cgctgctatg ctgcgcgtcg
     1381 acgccaatct gaaggcgtct cggttcgcct taccgttcgt cgtcgtgcga aatccaggtt
     1441 gcagaaacga gacgaaaata aagacggccg acagtgaacc tcggctgggg gggaaagctc
     1501 ggtcccgctg tcgaccgtca gcggtcttat tgcacagatt gggaggcaaa acccgaaatc
     1561 agtcactgac ctcgtgcggt cttcggtcac gcacgcggct ctgccggtgg gcactctttc
     1621 aggtcacatc cagcgcgcgc gtcggttact ttggttgcgt gtcacggccc atgcaggcga
     1681 gacacgtcct acagtcctga ctgcaaatca ctcctgggag cgaaatccca acttgccaat
     1741 ctgagcaaaa aaacgagatt gggcttaatc gctcatcaac ccgatagatg cgtctagcca
     1801 cgccagcatg gtgcgcgggt cggtcggctg ctctggtgca cgaaagtgtt ggctcactac
     1861 agcgtgagcc acgtgcggtc gaatcaaggt ttccttctcg ggaggctcgg ctgcataagc
     1921 ggctgcggcc gcgggaatct cgccgctctg acgttcgtcg gggggcggct cgggcacgtc
     1981 tagaatgtcc tctagccggc cctgctcgag ccctctctgc tcaatgccaa tctgcgcgac
     2041 ggcttcgccg tcatcatggg ccggatatcc cgacgccaag ggcgcgtcca attcgagtga
     2101 tactcgtgac gcttctggga actcgatcgt ctcacccact tgctcggggg tgggcccagc
     2161 gtccaaccct ggaaatggag gagcgagagg ggccggctcg agcaactcgt cgaacacggg
     2221 ggccgacgca gccaaaccgg cgggaattgg ggcttcttgc ggaccactct cgggaggcgg
     2281 cgtcttgagc gggacttcgc gcccttcctg aatggctacg gcctccgctt cagcctgggc
     2341 tacagcctcg tcgagtgtgc ccggaccctt gggacgatgc gacgaggatg gcggttgctc
     2401 ttcgtggaga tccgcgggcg gtgcctgagt gacccagcgg gcgccctcgg gtagcggcag
     2461 ctcttcggac acttcgccgc tcggaggcac cgactcgagc acgtccgaag acatctccag
     2521 gaaatcatct tcggtcagtt gctccgttgc ctcgatcgtc acctcgggcg cggctgcgac
     2581 cggttccggc ttctggctcg ccggggtcag gctgccatgc gccgcttccc agagagcatc
     2641 cgagctaccc gccgggcgct cgcgcgactc aggtgcggca ggaatgtcgg cacgagctgc
     2701 gacctcattg ggcgccggtg tactgggacg cggagtagtc cccggaggaa taggcgcgac
     2761 tgccggcggg gtgctggcct tgggcgccga accgggccca ccatcatccc aaaaaatatc
     2821 cgatagcgag ctcggatcgg gcgcccttcc agccgacgtc accaccggaa cgaccggggg
     2881 cggggctgcc gcgacgggca ttgctggaac gggcaccgcc gcaactggcg ctgcgggaac
     2941 tgaagccggc ggagcagcga cgggcggcga tgtacgagca cgcgccgcca acccaggcgg
     3001 cggttcactg agtgcgcgcg gcggagggat gggctcccgc gcgggcaccg tcgcaacccc
     3061 gggcgtgaac gccgggctcg cgatcgtgcc gacctgaacg tcagtccgtt tgggttcggc
     3121 cgccctgctc ttgtcggcag ctcgactgct gagctcggac gctctcccgt cgcgcggttc
     3181 ctgcgcgcgc cgagagaccc gatccaagag ggcgcgtagt ttttgaagag tctgcgggtt
     3241 gctcacgatg ccacccttcc cgatccgcgt tggagctcga ccagcacctg accgaacgag
     3301 gccccactat ccagggccgc gcgggcgtgg gccgcagctt gcgctcccgt caccaggaag
     3361 cgttcgacat tgtcatcacg tccgaggcaa accacgctga cgtcgcgccc gaaccctctt
     3421 tgaatcgcgc cctcgaggac cc
//
LOCUS       DASTKU010000762         9329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1042354, whole genome shotgun sequence.
ACCESSION   DASTKU010000762 DASTKU010000000
VERSION     DASTKU010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9329
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042354"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /gene="ssuD"
                     /locus_tag="VFQ61_26560"
     CDS             complement(<1..1127)
                     /gene="ssuD"
                     /locus_tag="VFQ61_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HET9958097.1"
                     /translation="MDVFWFLPTHGDGRYLGTAVGARAVTLPYLQQVARAADDLGFGG
                     VLVPTGRSCEDAWAVVSAVVPSTRRLKFLVALRPGIISPTAGARLAATVDRLSGGRLL
                     INVVTGGDPAESEGDGMFLEHDARYEAADEFLQIWRRVLAGETVDLEGKHLRVRGAKV
                     LYPPVQKPYPSLYFGGSSRAGHELAAKHADVYLSWGEPPEAVAAKIADVRERAAELGR
                     TVRFGIRLHVIVRKNRSDAWAAAEDLISHIDDNAIQAAQRSFARFDSEGQRRMSSLHG
                     GRRDRLEVSPDLWAGVGLVRGGAGTALVGDPDTVAKRMLEYRELGVDTFILSGYPHLE
                     EAYRTAELLFQKLPISHLGPRPELNLTGPFGEMIANDILPS"
     gene            1530..2717
                     /locus_tag="VFQ61_26565"
     CDS             1530..2717
                     /locus_tag="VFQ61_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9958098.1"
                     /translation="MSNAPEATQQTALETAERLARAFALTAVERDRLGGTAKRERDEL
                     RASGLLRLTIPRELGGLGGSLGQALQLVRVFARVDPSIAHLFGFHHLLLATVRLFGHR
                     EQWERSYRESAERPLFWGNALNPLDTGTRLQLDAEGRLSLRGRKSFCSGATDSDRLLI
                     SAHRATDERLVIGVVPSSRPGIRYLADWDNIGQRQTDSGSVEFDDVAIEESELLLSPG
                     PLSSPFAALRPCIAQLSLVNVYLGIAEGALEAAREYTQNQRRPWLNSGVEHAARDPYV
                     LHHYGEMWVSLEATRLLADEAGRALDAAFARGEALASEARGEVALKVAAAKAQSTRAS
                     LDVTARIFDVMGARSTTRRAGMDRFFRNTRTHTLHDPVDYKFKELGDWALNREYPKPS
                     FYS"
     gene            2746..3501
                     /gene="ribB"
                     /locus_tag="VFQ61_26570"
     CDS             2746..3501
                     /gene="ribB"
                     /locus_tag="VFQ61_26570"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HET9958099.1"
                     /translation="MKFQAVEAAIAAVSRGEIVVVADDPRRENEGDLILAASAATPEK
                     VAFMVRHTSGLLCVSARRQRLDALELPLMVPQNTESHQTAFTISVDYRVGTSTGISAE
                     DRSATIRALADAEMGPGEFARPGHVFPLRARDGGVLVRPGHTEAASDLAELAGLPPLG
                     ALAEIVNLDGSMARGVQLERFAQEYGLAFITIAELVEYRRATERVVALRPAHPALDDQ
                     PASVTGQELSLFGAARATPRRQAVLSAGQCPAR"
     gene            3667..4242
                     /locus_tag="VFQ61_26575"
     CDS             3667..4242
                     /locus_tag="VFQ61_26575"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9958100.1"
                     /translation="MSRLSAVAVTGNLSSPSRTVVLAKALLKALEEQHAFDSSLIDLA
                     TFARPLGQSLYRTELPPSIESALQTAEQADVLVVVTPVFRSSFSGLFKHFFDLFDLNA
                     LEEVPVILAATGGSERHTLVIEHSLRPLFSFFRAHTVPTTIFASERDFDSERRIAPEV
                     ILRAEQAARQATLLLRPRLPRVTSISRNLTV"
     gene            4364..5467
                     /gene="sfnG"
                     /locus_tag="VFQ61_26580"
     CDS             4364..5467
                     /gene="sfnG"
                     /locus_tag="VFQ61_26580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfone monooxygenase SfnG"
                     /protein_id="HET9958101.1"
                     /translation="MSNNEQQTPSELRFAYWVPNVSGGLVISKIEQRTSWDLDYNLKL
                     ARLAEKNGFDYALSQIRFTAGYGAEFQHESVAFSHALLAGTERLKVIAAILPGPWNPV
                     LAAKQLATIDILTGGRVAVNIVSGWFKGEFQAIGEPWLEHDERYRRSEEFIRALKGIW
                     TSDSFSFQGDFYRFRDYSLKPKPIQQPHPEIFQGGSSRAARDMASRVSDWYFTNGNTP
                     DGIRAQVDDIRAKAAKNGHSVKIGVNAFIIARDTEEEAKAVLREILQKADPEAVNAFG
                     HEVKQAGRASPEGEGNWSASTFEDLVQYNDGFRTNLIGTPHQIAERIVALKAVGVDLI
                     LAGFLHYLEEIEYFGKRVLPLVRELEAQRAKAA"
     gene            5509..6333
                     /locus_tag="VFQ61_26585"
     CDS             5509..6333
                     /locus_tag="VFQ61_26585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9958102.1"
                     /translation="MSTREPLLRVTDISLSFGSVRALAGVSFELERGEILALIGPNGA
                     GKSSLLNVINGLYRAGSGSIEFDSRSYEYMEPHAAAVAGVARTFQNLALFRRMTVIEN
                     VLSGRVLRTRVGLLRNALALPSARREEAEQRKHVEQVLELVGIREHEHAVVGKLPYGL
                     QKRVELARALASEPKLLLLDEPMAGMNSQEKGEMSRVIDEANRELGTSVILIEHDIGV
                     VMGISDRVVVLDYGRKIADGTPEQVGVDPVVIDAYLGVAHEEPAEPRAPAAKTGAN"
     gene            6333..7214
                     /locus_tag="VFQ61_26590"
     CDS             6333..7214
                     /locus_tag="VFQ61_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9958103.1"
                     /translation="MAFFLEVLLGGLLAGTMYSLVAIGFVLIYKASGVFNFAQGSMVL
                     FAALTFVSILEAGAPFWLAFTSSLLVMLILAVSIERVVLRPLVNRPPMTLFMATLGLS
                     FTLEGLAQTLFGAQVHGLDLGIEDTPLELLGISASKLDLVAASTAIGLVVLLAVLFNR
                     TRLGIALRAVADDPLAALAIGVDLRQIWGVVWAVAGGVGLVAGLLWGARLGVQFSLSL
                     IVLKALPVLIIGGFSSVLGTIVGGLLVGATEKLAEVYVGQLFGGGIENWFPYLLALGF
                     LLAKPTGLFGETQLERA"
     gene            7211..8287
                     /locus_tag="VFQ61_26595"
     CDS             7211..8287
                     /locus_tag="VFQ61_26595"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9958104.1"
                     /translation="MTMERNQVLTDSVLSLPRAKSEKSGRAARLALLTDRHRLLTTAI
                     LAVGFLVVPFVADDYSFSAILIPFLVLSLAGLGLNILTGYTGQLSLGSAAFMAVGAFA
                     TYNFQLRVPGIPLLVSLLLGGASSAVVGILFGLPSLRIKGFYLIVSTLAAQFFVEWAL
                     TKFAWFSNYNPSGVISAPPLRWFGQDLSSPAGRYLLTLVVVTGLTWVARNLVRSQTGR
                     NWMAVRDMDTAASVLGIPILRSKLEAFAVSSFYCGIAGALWAFAYLGTVEPHGFDLGR
                     SFQILFIVILGGLGSLVGPFLGAAFMVLLPILLNHVATGFFAGSVDPGHLENLQKVLF
                     GSLIIFFLIREPDGLARLLKWRKS"
     gene            8284..>9329
                     /locus_tag="VFQ61_26600"
     CDS             8284..>9329
                     /locus_tag="VFQ61_26600"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9958105.1"
                     /translation="MKTSLGWFNFGTASIALLPLLVSACKKPVENAEASPKQQVAAAK
                     PAAEGSGPAQYFPLQSYRVGPYAAGGTGFFGGFIDYMNLVNLRDGGVGGVKLTWSECE
                     TEYVVERGVECYERLKKGLDGAPTAATNPLSVGIAYATLDRSTTDKIPLITINHGRTD
                     STDGRVFPYVFPLQLNPYSEVSAIINYVGTREGGVEKLAGKKIVTLYHGSPYGKETQP
                     VLDILAKKYNFEIKHLEVPHPGNEQQSQWLEIKRFQPDWVILRGWGVMNPVALKTAAK
                     TGFNVERVIGNIWSNSEEDVRPAGSVGKGFISITTHPSGTDFPVITDIKKHVVDAGKG
                     NLADAKRFGSVYYNL"
BASE COUNT         1724 a         2791 c         2947 g         1867 t
ORIGIN      
        1 tggcttggca aaatgtcgtt tgcgatcatc tcgccgaaag gtccggtcag gttcaattcc
       61 gggcgaggtc ccaagtgtga aattggcagt ttctggaaaa ggagctcggc ggttcggtac
      121 gcctcttcca aatgtggata acccgacaga atgaaggtat ccacgccaag ctctcggtat
      181 tccagcatgc gtttcgctac cgtatcggga tcgccgacca gcgccgtgcc ggcccctccg
      241 cgcaccagtc caacacccgc ccagagatcg gggctcactt cgagtcgatc gcgccgacct
      301 ccgtggagac tcgacatgcg cctttgaccc tctgagtcga agcgggcgaa ggagcgttgc
      361 gcggcttgga tggcgttgtc atcgatgtgg ctaattaggt cttcggccgc ggcccaggcg
      421 tcgctgcgat tcttgcgaac gatgacgtgt aggcgaatcc cgaaccgtac ggtgcgccct
      481 agctcggccg ctcgctcacg aacatccgcg atcttcgcgg ccactgcctc cggcggctca
      541 ccccaactca gatacacgtc ggcgtgcttc gcggcgagct catggccggc ccgcgacgag
      601 ccgccgaagt acaaggatgg ataaggcttc tgaacgggtg ggtagagtac cttcgctccg
      661 cgaacccgca ggtgtttacc ttcgagatct acggtctcac ccgccagcac gcgccgccag
      721 atctggagga actcgtcggc cgcctcgtaa cgcgcgtcgt gttcgaggaa catgccgtct
      781 ccctccgact cggccggatc gccgccggtc accacgttaa tcagcaatcg acctcccgag
      841 agccgatcta ccgtggcggc caagcgcgcg ccagccgtag gggagatgat accgggccgt
      901 agcgcgacta ggaacttgag ccgccgtgtg cttggtacaa ccgccgaaac cactgcccag
      961 gcgtcttcac acgagcgccc cgtcggcact agtactccgc cgaatcccag atcgtcagcc
     1021 gcccgagcca cctgctgcag atacggcagc gtgacggcgc gcgcgcccac agccgtgccc
     1081 agatagcggc catccccatg cgtcggtaag aaccaaaaaa cgtccattgt tgccctctgc
     1141 tgagccgcac gatcgtcgag tcgacgtggt tcgagcgccg aggtgccgcg ttcctgagtg
     1201 aagcggtgag ccgcatacgc aacgagctgg tttgtcgtgt gctctagcct ggtaaaggtc
     1261 gggactagca agtcctgggc cgagcacacg cgcctgcgtt tcgtgtcatg agcccggcgc
     1321 gtggcaaaaa tcgggagctc gtctgctgct ccagcaacac ctcgcggaca gcatctgtat
     1381 ggagagcagc atgatttcgg caaatggttg acgtcgatag cgaactggaa ggactcgcaa
     1441 acccggcgag ttgtcctctc cgagcggcta cagagcggca ttcgttcgaa ggactcaagt
     1501 ggcacgttgt gtgcgtatgc ctcgcccgca tgtcgaatgc tcccgaggcc acgcaacaaa
     1561 ccgcgctcga aacggccgaa cggctcgcac gagcctttgc gctcaccgcg gtcgagcgag
     1621 atcgtttagg gggtacggcc aagcgagagc gcgacgagct gcgagcgagc gggctattga
     1681 gattgacgat cccgcgtgaa ctcggaggcc tggggggatc gctcgggcaa gccctccaat
     1741 tggttcgggt gtttgcgcgc gtggatccct cgatcgcaca tctgttcgga ttccatcacc
     1801 tgctgctcgc gaccgtgcgt ctatttggtc atagagagca atgggagcgc tcataccgcg
     1861 agagcgcgga gcgccccctg ttttggggca acgctctgaa cccattggac accggcacgc
     1921 gcctccagct ggacgcggaa ggtcggcttt cattgcgagg tagaaagagc ttttgttccg
     1981 gggccactga ctcggaccgc ctattgatct cggcgcatcg cgccacggat gagcggctgg
     2041 tgattggagt agttccgtct tcgcggcctg gcattcgata tctcgcggac tgggacaata
     2101 ttgggcagcg tcagaccgac agcggcagcg tggagttcga cgacgtcgcg atcgaagaat
     2161 ccgagttgct cttgagcccc gggccgctga gcagcccatt cgcagcgctg cgtccctgca
     2221 ttgcccagct cagcttggtc aacgtgtacc tcggtattgc cgaaggcgca ctcgaggctg
     2281 ctcgcgaata cacgcagaat cagcgccgac cctggctgaa ctccggagtc gagcacgcgg
     2341 ctcgggaccc atacgtgctg catcactacg gagaaatgtg ggtctcgctc gaagccacgc
     2401 ggctgctcgc ggacgaagcg ggccgcgctc tcgatgcggc tttcgcccga ggtgaagcac
     2461 tcgcaagtga ggcacgaggt gaggtggccc tcaaagttgc agccgcgaag gcgcagagca
     2521 ctcgcgcgag tctagatgtc accgcgcgga tcttcgacgt gatgggggct cgctctacga
     2581 cgcgacgggc gggcatggat cgcttcttcc gaaacacgcg cacgcatacc ctccacgatc
     2641 cggtcgacta caagttcaaa gagttgggag actgggccct gaatcgcgaa tacccgaaac
     2701 cgagcttcta ctcctgaaca cgttgcctct tcattcggag aaaagatgaa attccaagca
     2761 gtcgaggcgg ccattgccgc tgtctcccgc ggtgaaatag tcgtggtggc tgacgacccg
     2821 cgccgagaaa atgagggcga cctaatcctc gccgccagcg ccgcgacccc cgagaaggtg
     2881 gcgttcatgg tccgccacac gagcggccta ctgtgcgtgt ccgcgcggcg acagcgtttg
     2941 gatgcgctcg agttgccttt gatggtcccc cagaataccg agtctcatca gacggcattc
     3001 accatctcgg tcgattatag ggttggcacc agcaccggaa tctcggccga ggaccgcagc
     3061 gcaacgattc gggccttggc tgacgcggag atggggcctg gtgagtttgc acgccctggc
     3121 cacgtgttcc cgttgcgcgc acgcgacgga ggcgtgctgg ttcgtcccgg gcacaccgaa
     3181 gcggcgagcg acctagcgga gctcgcgggc ctgcccccgc tcggcgctct ggcggaaata
     3241 gtgaatctgg atggcagcat ggcgcgcggc gttcagctcg agcgcttcgc gcaggagtac
     3301 gggctcgcat tcatcaccat cgccgagctt gtggagtacc ggcgcgccac cgagcgcgtc
     3361 gtagcactgc gcccagcgca tcccgctctc gacgaccaac cggcgagtgt caccgggcag
     3421 gagctctcct tgtttggtgc tgctcgcgca acacctcgca ggcaggctgt gttgtctgct
     3481 ggtcagtgcc cggcgagata gcgcagaaag tagcgtggta cgagacctgc actcgagcgc
     3541 gtgtcctacg tcaatccaag cccagcgagc ttcgttcgat cgtgagttcg attttagccc
     3601 gttgggatgc gcttgggcat cggatatcca acgtagaacc gcggccggaa accttgggag
     3661 tacaccatga gtcgattgag cgcagtcgca gtgaccggaa acctttcgag cccctcgcgc
     3721 accgtcgtgc tggctaaagc cctcctgaag gcgttggaag agcagcacgc cttcgactcc
     3781 tcactgattg atcttgccac cttcgcgcgc ccactgggcc aatctttgta ccgaaccgaa
     3841 ctgccgccat cgatcgagag cgcgctccag accgcagagc aagccgatgt tttggtcgtg
     3901 gttaccccgg tctttcgcag ttcattttcc ggcctgttca agcacttctt cgatttgttc
     3961 gacctgaacg cgctggaaga ggtgcccgta attctggcgg ccacgggcgg cagtgagcgt
     4021 catacgctcg tgatcgagca ctcgctccgt ccgctcttct cgttctttcg agcccatacc
     4081 gtaccgacca cgatctttgc cagcgaacgg gacttcgata gcgagcgtcg cattgcaccc
     4141 gaagtcattt tgcgcgccga gcaagcggca cggcaggcaa cgctcttgtt gcgcccacgg
     4201 cttccgcgcg tgacgtccat atcgcgcaac ctgactgttt gaaattcaag tcgtgggcgc
     4261 gtgggcgcgc ctgcgtgctg ccacgtgcgg ggagagctgc gtcgaaccag acacgcgcgc
     4321 caattcaaaa actcgaatac tcaaggactg gatggatact cagatgagca ataatgaaca
     4381 acaaacgccg agcgaactgc gattcgcata ctgggtgccg aacgtgagtg gcggcctcgt
     4441 gattagcaag atcgaacaga ggaccagctg ggatctcgac tacaacctga agctggcccg
     4501 tctcgcggaa aagaacggct tcgactacgc tctcagccag atccgattta cggccggcta
     4561 tggcgcggag ttccagcacg aatcggtggc attctcgcac gcgctcttgg cgggcacgga
     4621 acggttgaag gtgattgcgg ctatcttacc tgggccctgg aatccggtgc tcgcggccaa
     4681 gcaactcgcc accatcgata tcctgaccgg tggacgcgtg gccgtgaaca tcgtgagtgg
     4741 ttggttcaag ggagaatttc aagccatcgg cgagccgtgg ctcgagcacg acgaacgcta
     4801 ccgtcgctcc gaggagttca ttcgagcgct gaaggggatc tggacgagcg atagcttctc
     4861 ctttcagggc gatttctacc gttttcgcga ctattcactg aagccgaagc ccattcaaca
     4921 gccccacccc gagatcttcc aaggtggtag ttcgcgggcg gcgcgagaca tggcatcgcg
     4981 agtttcggac tggtacttca ccaacggcaa taccccggac gggatccgcg cccaagtaga
     5041 cgacatccgg gccaaggcgg ccaagaacgg tcactccgtg aagattgggg taaacgcctt
     5101 catcatcgcc cgcgatacgg aggaagaggc caaagcggtg ctgcgcgaga tcctgcagaa
     5161 ggcggatcca gaggcggtca acgctttcgg ccacgaggtg aagcaagcgg gcagagcctc
     5221 acccgagggc gaaggcaact ggtccgcctc gacgttcgag gatctcgttc aatacaacga
     5281 cggcttccga accaacttga tcggtacgcc acaccagatc gcggaacgca tcgtggccct
     5341 caaagccgtg ggcgtcgacc taatcctggc gggctttctg cattacctcg aggagatcga
     5401 atacttcgga aagcgtgtat tgccgctggt gcgcgagctc gaggcccagc gagccaaagc
     5461 ggcctgagcg ttcgcgactc tcaccactcg ctatctggaa ttgaaatagt gtccactcga
     5521 gagccgctcc tgcgcgtgac ggatatctca ttgtcgttcg gatcggttcg cgccttggcc
     5581 ggcgtgagct tcgagctcga gcgcggcgag atcttggccc tgatcggtcc caacggtgca
     5641 ggcaagagct cgctcctgaa cgtgatcaac gggctctacc gagcggggag cggctcgatc
     5701 gagttcgata gccgcagtta tgagtacatg gagccacacg cggccgccgt ggccggcgta
     5761 gcgcgaacct ttcaaaactt ggccctgttt cggcgcatga cggtgatcga gaatgtgctc
     5821 agcgggcgtg tattgaggac gcgcgtggga ctgctgcgaa acgcgctcgc gctgccgtcg
     5881 gcgcgtcgcg aagaagccga gcaacgcaag catgtggagc aagtgctcga gctggtcggc
     5941 atcagagagc acgagcacgc ggtggtgggc aaactgccat acggtttgca aaagcgggtc
     6001 gagctggcgc gagcgctcgc gagcgagccg aagctgttgc tgctcgacga gccgatggcg
     6061 ggcatgaact cgcaagagaa gggcgagatg agccgcgtca tcgacgaggc aaatcgcgag
     6121 cttgggacga gcgtcatctt gatcgagcac gatattggcg tggtcatggg catctcggat
     6181 cgcgtcgtcg tgctcgacta cgggcgcaag atcgcggacg gcacacccga gcaggtcggt
     6241 gtagatccgg tggtgatcga cgcctacctc ggtgttgcgc atgaagagcc agcggaacca
     6301 cgggctccgg cggcgaagac gggagccaac tgatggcctt ctttctcgag gtgctgctcg
     6361 gcggcctgct ggcgggcacc atgtactcac tggtcgcaat cgggttcgtg ctgatttaca
     6421 aggcctccgg cgtcttcaac ttcgcgcaag gctcgatggt gctattcgcg gcgctcacgt
     6481 tcgtgagcat cctggaagcg ggcgcgccgt tttggttggc gttcacatcg agcttgctgg
     6541 tcatgctgat tctggccgta agcatcgagc gcgtggtgct gcgaccgctc gtcaatcggc
     6601 ctccaatgac gttgttcatg gctacgctcg ggctgagctt tacgctggaa ggcttggcgc
     6661 aaacgttgtt cggggcgcag gtacatggcc tcgatttggg aatcgaggac acccccctcg
     6721 agctactcgg gatctcggcg agtaagctcg atttggtggc cgccagcact gcgattggtc
     6781 ttgtcgtctt gcttgcggtg ctgttcaatc gaactcggct cgggatcgcg ctgcgcgccg
     6841 tcgccgacga tccactcgcc gcgctggcta ttggtgtgga cctgcgccaa atctggggtg
     6901 tagtctgggc cgtagctggc ggagtcgggc tcgtggcagg cttactttgg ggtgcgcgcc
     6961 tgggcgtaca attctcactg tctctgatcg tgctgaaggc cttaccggtc ttgatcatcg
     7021 ggggcttctc ttcggtgttg ggcacgatcg tgggcggcct cttggtgggc gctacggaga
     7081 agctcgccga ggtgtacgtg ggacaactat tcgggggtgg gatcgaaaac tggttcccgt
     7141 acttgttggc gctgggcttt ctgcttgcca agcccacggg cttgttcggc gaaactcagc
     7201 tggagcgtgc atgaccatgg aacgcaatca ggtcttgact gattcggtgt tatcgctgcc
     7261 gcgcgccaag tccgagaaat cggggcgggc cgcgcgcctc gcgctcctaa ccgacaggca
     7321 tcgactgctc accaccgcca tcttggcggt cgggttcttg gtggtgccgt tcgtggctga
     7381 cgactactcg ttcagtgcca ttctgatccc gttcttggtg ttgtcgctgg ccgggctcgg
     7441 gttgaatatc ctcacgggtt ataccggtca gctttcgctc ggttcggccg cgttcatggc
     7501 cgtcggcgct ttcgcgactt acaacttcca actgcgcgtg ccgggtatcc cgctgctggt
     7561 gagcctgctg ctcggtgggg cgagctcggc cgtcgtgggc attctattcg gcttgccgag
     7621 cttgcgaatc aaaggcttct acctgattgt ctccaccctt gccgcgcagt tcttcgtgga
     7681 gtgggcgctc accaagtttg cttggttctc gaactacaat ccttcgggcg taatcagtgc
     7741 gccgccgcta cgctggttcg gacaggacct gagctcgccg gccggacgct acttgctcac
     7801 gttggtggtc gtcacggggc tgacctgggt ggcgcgcaac ttggtgcgca gtcaaaccgg
     7861 gcgcaactgg atggccgtgc gagatatgga tacggcggca tcggtgctgg gcattccgat
     7921 cttacgctcc aagctggagg ccttcgcggt gagctccttc tattgcggga tcgcaggtgc
     7981 gctttgggcc tttgcgtact tgggcaccgt cgagccgcac ggcttcgatc tcgggcgctc
     8041 gtttcagatc ctgttcatcg tgattttggg tgggttgggc agcttggtgg ggccgttctt
     8101 gggcgccgcc ttcatggtgc ttttgccgat tttgctcaat cacgtggcga cggggttctt
     8161 tgccggttcg gtcgaccccg ggcacctgga gaatctgcag aaggtgctct tcggaagctt
     8221 gatcatattc tttctgattc gagagcccga cgggctcgca cgtttgttga agtggaggaa
     8281 atcgtgaaga catcgttggg ttggttcaat ttcggtaccg catctatcgc gctgctgccg
     8341 ctcctggttt cggcgtgcaa gaagccggtc gagaacgcgg aagccagccc caagcaacaa
     8401 gtcgcggccg cgaagccagc cgctgaaggc agcggcccgg cgcagtactt tcccctgcag
     8461 agttatcgag tggggcccta cgcggccgga ggcactggct tcttcggtgg cttcatcgac
     8521 tatatgaacc ttgtcaatct ccgggacggc ggtgttggcg gcgtgaagtt gacttggtcc
     8581 gaatgcgaaa ccgagtacgt cgtcgagcgt ggcgtcgagt gctacgaacg gctgaagaag
     8641 ggtttggacg gcgcacccac cgcggctacg aatccgctca gcgtggggat cgcgtacgcc
     8701 accctggatc gttcgactac cgacaaaatt ccgctcatca ccatcaatca cggtcgtact
     8761 gactctaccg atggccgggt gttcccctat gtcttcccgc tgcagctcaa tccgtacagc
     8821 gaagtctcag ccatcatcaa ttacgttggc acccgcgagg gcggggtcga gaagctcgcc
     8881 ggcaagaaga tcgtcaccct gtaccatggc tccccttacg gcaaagagac gcagccggta
     8941 ctcgatatct tggccaagaa gtacaatttc gagatcaagc acctcgaagt cccccacccg
     9001 ggcaacgagc agcaatcgca gtggctcgag ataaagcggt ttcagccgga ttgggtgatc
     9061 ctgagaggct ggggcgtgat gaacccggtg gccttgaaga cggcggccaa gaccggcttc
     9121 aacgtcgagc gggtgattgg caatatctgg agcaactccg aggaggacgt gcgccctgcc
     9181 ggcagcgtgg gcaagggctt tatctcgatc acgacccacc cttcgggcac ggacttcccc
     9241 gtaatcaccg acatcaagaa gcacgtggtg gatgcgggca aagggaacct cgcagatgcc
     9301 aagcgtttcg ggagcgtcta ctacaatct
//
LOCUS       DASTKU010000763         4837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1266512, whole genome shotgun sequence.
ACCESSION   DASTKU010000763 DASTKU010000000
VERSION     DASTKU010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266512"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..855)
                     /locus_tag="VFQ61_26605"
     CDS             complement(178..855)
                     /locus_tag="VFQ61_26605"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9958106.1"
                     /translation="MQHAQQPGMQPPGLQQPGGYPSPGREEFPSSPEREPSYDRVPGL
                     GPAQDYGARAPEYGAPRQSNPPPLEPQYQQPQPGFQPGAQAGYPGRGQAPVAGPAPQA
                     AAIPAARDPELVEAGAQRSLAGFLVSYDGQELGVFWPFYQGPNMLGRKGAAPGLDIEI
                     DHPTTSSRHAVLYASARPARLKIEDLGSTNGTFAQDRQLERGKKHEVRDGDVVRFGGF
                     SVIVKLV"
     gene            complement(1995..2351)
                     /gene="rplS"
                     /locus_tag="VFQ61_26610"
     CDS             complement(1995..2351)
                     /gene="rplS"
                     /locus_tag="VFQ61_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET9958107.1"
                     /translation="MATLSKIAAFEAESLRKDLPEFRVGDTVAVHYKIIEGDKERVQV
                     FRGVVIKRHRSGARSTFTVRKVSFNVGVERVFMLHSPRLEQIEVVSRGVVRRARLFYL
                     RDLSGKAARVQDEKDR"
     gene            2538..3206
                     /gene="rplC"
                     /locus_tag="VFQ61_26615"
     CDS             2538..3206
                     /gene="rplC"
                     /locus_tag="VFQ61_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET9958108.1"
                     /translation="MNQHPGVIGRKLGMTQVFSEDGSVIACTVVESKPVVVSKRTKEK
                     DGYDALVLGLEERSERHTNKPLTGFYKKVNTSPKETLRELRCSAEFAAGFELGQEVKL
                     EQIFEVGQFVDVQGVTRGHGFSGVVVRHNFAGNVNTHGTHEYRRHGGSIGTRMTPGRV
                     KLGMKMPGQHGNKTFSVMNQKVVKIVPEQNLLLIHGGVPGSRDSIVVVRGAVRRRGGK
                     PAAK"
     gene            3231..>4837
                     /locus_tag="VFQ61_26620"
     CDS             3231..>4837
                     /locus_tag="VFQ61_26620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9958109.1"
                     /translation="MLEGSPFQPWVPERILLERAGLLFVDKPSGIPTHGGHADVAEDV
                     VRRLALVLERRGEDPYLGVHQRLDQGTSGVLVFVRDAALNAAVKDCFETDRVLKRYVA
                     AVELQDGSPLWRRERLTLSHRLELVDGRQREVARGGKPCLAECRVLRRVGCRALVALE
                     PKTGRTHQLRAQLALVHAPIAGDDLYGSVRAPRLLLHAQSLQLVGLAPAVQSEVPSLF
                     EGWIEGRAEGLGDARELERKLADAASRRFPLVQRTQALRWVNSAADALPGLEIDWYDG
                     YVTLSPSTEEATERVPEIASLLLQAGARGVYLKQRARADLRRLDASELAPPVPIVGEA
                     APESFDVREESWRIAVQLADGLSTGLFLDQRENREFLRQRVAGQEVLNLFAYTGSFSV
                     AAALGGARRVTTVDLSKRAVERARRNFELNGLPLESHRFFAEDAVRFLDRAARRGDTY
                     DWVVLDPPSFASNKSGKVLSVARDYAELVARCVRVLGPGGNLLAVTNHRGTRPEALRS
                     MLADVARREGRTIEKLKSLASALDFPPT"
BASE COUNT          852 a         1542 c         1638 g          805 t
ORIGIN      
        1 ggatcgattc gcagcaggcg cgcctggggc gcggcgagcg cggtggcagt gccgatcaga
       61 ccagcaaaaa gcacgagcag cgccgtggca tgcgcgcgcg aaaccagcga gcggaccaac
      121 cgaatcaaca tgttagaggt caccttcgaa ccttcggtag cgagcggcga gccgcggcta
      181 cacgagcttg acgatcacgg agaatccccc gaatcgaacc acatctccgt cccggacttc
      241 gtgctttttt ccgcgttcga gctgacgatc ttgggcgaac gtcccgttgg tgcttccgag
      301 atcttcgatc ttcaggcggg ccggtcgcgc ggaggcgtac agtaccgcgt gacgtgagga
      361 cgtggtcggg tgatcgattt cgatgtctag gccgggcgct gcacccttac gcccgagcat
      421 gttcggacct tgataaaagg gccaaaacac gccgagttcc tgcccatcgt agctgaccag
      481 aaagcccgcg agcgagcgct gcgcaccggc ctccacgagc tccggatcac gagccgctgg
      541 gatagctgcc gcttgggggg caggccctgc aaccggcgct tgccctcgac cgggataccc
      601 ggcttgcgcg cccggctgaa aaccgggttg aggttgctga tactggggct ccagcggcgg
      661 cgggttcgac tgtcgtggcg ctccgtactc gggggcccgc gcgccgtaat cttgagcggg
      721 acctaggcca ggaacgcggt cgtaacttgg ctcgcgctca ggggacgaag ggaactcctc
      781 gcgcccggga ctcggataac caccaggctg ttggagtcct ggcggctgca ttccgggctg
      841 ttgagcatgc tgcatatcaa acccgggaaa cggcggcggt ggcgtgggac cacgcgctgc
      901 gcctcccgct cctgcgccgg ggaacgcggg cggatattgc tgtggcgctg cgccgggaaa
      961 acccccagga ctgtaggggg actgctgcgg agcgttgccg aattgcgaac cgttcggcaa
     1021 cggcggcggc gaggaaccac tcgccccggg atatcctcct ggagcagggc ccggatgaga
     1081 gccgctcgca cccggatgcg aaccgccggc gaaaggtgac gccgcatacg gcgatgcggc
     1141 agggctgccc gggtatgcag gcgggttggg cgatgctccc ggataggagc cgcgctcagg
     1201 ggcctgttga ggggcagaag gttgcggaag cggcggcaat ggagggccac ccattccgcc
     1261 cgcattctgc ggaaaatgcg gttgcccgta gcccggacct tgcgccgacc cggaagacga
     1321 gccctgcgca ggtaaaccct gtccgggtaa accttgtccg ggtaaaccct gcccatagtt
     1381 cgcgaggggc ggaagcggcg gaggcgctcc gggggatgaa cctccgaagc ctggagaaga
     1441 gccgccaaag ccctgagcgc cgccgggacc tccggggcta ttcgctccag gatagtttgc
     1501 aagcggcgga aggggcggag gcgcacccgg gtgttgcgct cccggtgggg caggatagtt
     1561 ctccaccggt ggccccatcg tcgcgccgaa cggattcaac ccgtcgggcg aaccaatccg
     1621 taaccgctca tccgattcag cctcggttgc tgtaccgata ttcggcggcg gaccgtaacc
     1681 cggtggaccc caagctgcgg gaggttgcgc acccggcggg gcagcttgct gtcctccggg
     1741 gaattgtcct ccaggaaact gtgggggacg agcttgttga ggcggcgttg gagaaggaac
     1801 cccagctgat gcagccggca acggcgcgcc gcaggaaagg caatatttcc cgctcgtctg
     1861 gttggtggcg ccacacttcg cgcactggat catcgaatga ctccaggacg cagcatcgcc
     1921 gcttctgcgg tcgggagcaa gcatggtcga gccagagcgt gaagaaagct ctggctcgac
     1981 tgctcacgcg gcgctcagcg gtctttttcg tcctgcacgc gcgcggcttt gccggacaga
     2041 tcgcggaggt agaacaagcg cgcacgccgc actacgcctc gcgaaacgac ctcgatctgc
     2101 tcgaggcgag gagagtgcaa catgaacacg cgctcgacgc caacgttgaa gctcaccttg
     2161 cgaacggtga acgtcgaacg cgcgccggag cggtgacgct tgatgaccac cccacggaag
     2221 acttggacgc gttccttgtc cccttcgatg atcttgtagt gaactgcgac ggtgtcaccg
     2281 acgcgaaact cgggaaggtc cttgcgaagg ctttcggctt cgaaggctgc aatcttggag
     2341 agggtggcca taggctttta gtcccggtcg aacgggcggg ggattatgca cccgagagag
     2401 gcgccgtcaa gcattccctt acatcctccc cttgacgagc ctgccttctg cagctaatcc
     2461 gcgtgccact aaacctcata ccagtggctc gggcacgctt cgcggttccg agcgcggcga
     2521 cgaaggactc tccccccatg aatcagcacc ccggcgtaat cgggcgcaag ctcggcatga
     2581 cgcaggtctt cagcgaagac ggctctgtca tcgcctgcac ggtcgtcgaa tcgaaacccg
     2641 tcgtcgtgag caagcgcacg aaagagaagg acggctacga cgcgctcgtg ctcggcctcg
     2701 aagagcgcag cgagcgacac accaacaagc ccctgaccgg cttctacaag aaggtcaaca
     2761 cttcgccgaa agagacgctg cgcgagctcc gttgcagcgc tgagttcgcg gcgggcttcg
     2821 agctgggcca agaggtcaag ctcgagcaga tcttcgaggt gggtcagttc gtggacgtgc
     2881 agggtgtcac ccgcggtcac ggcttctccg gtgtcgtcgt gcgccacaac ttcgccggta
     2941 acgtgaacac ccacggtacc cacgagtacc gccgtcacgg tggttcgatc ggtacgcgca
     3001 tgacccccgg ccgcgtgaag ctcggcatga agatgccggg tcagcacggc aacaagacct
     3061 tcagcgtcat gaaccagaag gtcgtcaaga tcgtccccga gcagaacctg cttttgatcc
     3121 acggtggcgt gccgggatct cgtgactcga tcgtcgtggt gcgtggtgcc gtacgccgtc
     3181 gcggcggcaa gcccgcggcg aagtaaccgc tcgcgttcag caagaagccg ttgctagaag
     3241 gctcgccttt tcagccctgg gtgccagagc gcatcctgct ggagagagcg ggccttttgt
     3301 tcgtcgacaa gccgagcggc attcccacgc acggcgggca cgcggacgtc gcggaagacg
     3361 tggtgcgtcg gctcgcgctc gtgttggagc gccgcggcga ggacccctac ttgggggtcc
     3421 accaacggct cgatcaaggc acctcgggcg tgctcgtgtt cgtgcgggac gcggcgctga
     3481 acgccgcggt caaggactgc ttcgagaccg atcgcgtgct gaagcgctat gtcgccgcgg
     3541 tcgaactgca ggatggctcg ccgctttggc gccgtgaacg gctcacgctc tcgcatcgac
     3601 tcgagctcgt ggacgggaga caacgtgagg tcgcacgcgg cggcaagccg tgcctcgctg
     3661 aatgtcgagt gctgcgtcgc gtgggctgcc gagctctggt cgcgctcgag ccgaagacgg
     3721 gtcgtaccca ccagcttcga gcccagctcg cgctcgttca cgcgccgatc gcgggggacg
     3781 atctgtacgg ctcggtgcgt gcgccgcgct tgctactcca cgcgcagagc ctgcaactcg
     3841 tgggcttggc tcccgcggtg cagagcgaag ttccgtcgct attcgaaggc tggatcgaag
     3901 gacgggccga agggctcgga gatgcgcgcg agctcgaacg caagctcgcg gacgcggcca
     3961 gccgtcgttt cccgctcgtg cagcggacgc aggccctgcg ctgggtcaac tccgctgcgg
     4021 acgcgttgcc agggctcgag atcgattggt acgacggcta cgtcacgctc tccccgtcca
     4081 ccgaagaagc caccgagcgc gtgcccgaga tcgcgagcct gctgctgcag gcgggtgcgc
     4141 gcggcgttta cctgaaacaa cgggcgcgcg cggacttgcg gcggctggat gccagcgaac
     4201 tcgcgccgcc ggtgccgatc gtgggcgaag cggcccctga atccttcgac gtacgcgaag
     4261 agtcttggcg catcgccgtg cagctcgcgg acgggctctc gacgggccta tttctcgatc
     4321 aacgcgagaa cagagagttt ctgcggcaac gcgtggcggg ccaagaagtc ttgaacctgt
     4381 tcgcgtacac tggctcgttc agcgtcgcgg cggcactggg cggtgcccga cgcgtcacga
     4441 cggtagatct ctcgaagcga gccgtggagc gcgcgcgtcg taatttcgag ctcaacggct
     4501 tgccgctcga gtcgcaccgc ttcttcgccg aagacgcggt gcgctttctg gatcgagccg
     4561 cccggcgcgg cgacacctac gactgggtcg tgctcgatcc accgagcttc gccagcaaca
     4621 agtccggcaa ggtcttgagc gtcgcccgtg actacgcgga actcgtggcg cgctgcgtgc
     4681 gcgtgctcgg cccaggaggc aacctgctcg ccgtcaccaa tcatcggggc acccgccccg
     4741 aagctttgcg cagcatgctc gcggacgtgg cccgccgcga gggtcgcacc atcgagaagc
     4801 tcaagagctt agcttcggcc ctggatttcc cgcccac
//
LOCUS       DASTKU010000764         9589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_596017, whole genome shotgun sequence.
ACCESSION   DASTKU010000764 DASTKU010000000
VERSION     DASTKU010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9589
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596017"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5052)
                     /locus_tag="VFQ61_26625"
     CDS             complement(<1..5052)
                     /locus_tag="VFQ61_26625"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HET9958110.1"
                     /translation="MGALPQLASLDPQSTKTIASADQSRSAECESRTKATPLTKLISP
                     VSRTPDPFEALVSKALGVSREQSASRVPGGSADLLFEAFRGRGALNTCDANAAPSPAL
                     LPARPAVEDREATYSEVAYPDRESTLRFEGATLIVPPGAVAHEERLTMRTVADREAGA
                     LDTGMANVTPGQRAFRLGPHGLRFLRPVTLILPFNRTLMPAGMTAQDLGTFFFDAESG
                     SWRPIRVREVREAEGLVVAETEHFTDFINATLAVPDHPTTASHETNTIADLKLGDPAT
                     GLEPMTTPTGGSDGAAHVSYKFTLPPGRRGLGPDLQLSYSSDAGNGWMGLGWDIGLSR
                     VRIDSRFGVPRYDSSLETELYALDGSELTPRGATSGPRERQLHPAPREAEREFVRRAE
                     GSFARIVRHGSTPTAGPNAYWWELTDKNGTRFRYGVDAASRLSDSDTGRIAEWGLSEV
                     EDTYGNRVHYEYLLDSGTLGAGGQKWVQMYPKEIRYTDHGSEAGAMYVQFVLGAAGQR
                     PDVSSSGRSGFLVRTARRLEAMDVFYSPGKRPPAPETSAAIGPLLRRYAFTYKTGEFS
                     KSLLEAIELQAPDMNGELQFRWSRRHKTAFTYFERNPSIQPGFSGALPWGTASDKDVL
                     NASSGEQGGTNGFLGLGICGAFHAGVGGGLTGGNDRTSRTFLDFNGDGLPDFLSGGRA
                     LWGGASEVTGYRAASISGLEDTFWETEQSTRSLNVGVHVAGDRGEAGGNWAWTNSKQQ
                     GGFADVDGDGFVDWVSPFLGLIYRNDGRGGLAPLGRTLTGRSSVVPDALRRSIEESAP
                     YADPLVRWVAPYTGTVALSGAITRKTAGGGDVVAEIWRDGDGSPLWSRAFGSTDATAC
                     SPGGTTGSCDGGLTLTVNAGQAVFFRTQALGDMAGTEMLWDPLLSYQSVCQAGFCTAV
                     SPSAHSELEPWGAPVYDFSQARDFRLAGPPNQAWTTAPLGTVNVSVQNGVFQKIAPTA
                     DDVTVRVVRVDSLTGAETELYARTFAAAATTRVDVGSAVPTSIAMTASAALDASGAPA
                     DFKADLLRFEVVSDSPIDPSAVAWSPEVWYQEYCRTPPTENASPVCGALHCPEDGSDT
                     CTMDGDPTPERPLPRRLLQQPAPVTYSTYVVRGAPDGAALSENLPTRAWAAPETSTFE
                     ATVSYALAASSAAAGADDAVLLIQGNQRLLGKIRVPAGASGVQVLTGLTMTAGEPIYL
                     TALRKGNQEMFWNVALRSESSGINYPTPVLNSHRVAAWNDLNERGVMSGGYHQWSTGQ
                     WTHKTASSPALSNAVIEDRRQYETKQGAEDLPFRPALVSPRGIAPNPGQLVAGSPLPA
                     WISSGLDLYIAAGELKPSRVGANPLALVRAAQAPAFRKSHGSSSAINLSATVVDFSNT
                     GSTSEADVDLIDLNGDGYPDSFDTGSVQYGDGQGSFGPDQPRDFGGGFRKFEGSNRRY
                     GLKPGSGAAGISERLGASGTVQSILNAVPSFGRTYGRTRTTLDLIDINGDGLPDRVER
                     ENLGADAQVRLNLGYSFGPPFALPMARFGSTIGDEGLGDTTSKANEEAALQLQTNVAN
                     SYQIGYGGVGGGLAYTSTRTLIDFADVTGDGLPDRVLKMPGRSRLYVQINTGEGFAPE
                     QVWRMPDWGAELPSNGHFLGLGSRDVLAFSEGTEYHLGIGGAAEIPLFWALCLAVEAS
                     EQFVHNTLDAELVFQD"
     gene            complement(5228..7231)
                     /locus_tag="VFQ61_26630"
     CDS             complement(5228..7231)
                     /locus_tag="VFQ61_26630"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958111.1"
                     /translation="MSVFHVRKVRMFGLALALGVAACDGLPRDEGQIWTCFGLVHETA
                     PGRHEQSPLYYGGCSPDDPGLQFVTYARQAASEAEVIAACDRSCGDAFARYFRTHPEA
                     ALPLSCETFFVTPCEDPRLGADLTRLPANLNADYFSGGPADFRTAIAGTLHLNLDGAQ
                     ASVPGAGVVDVTYAPCQGAGQSCAVDVSRFDAVAAEPFELGGALVTAAQVQMQGRSAG
                     RQSGPEFLLPSGSVRVEGNFTLDGERASVHASNDHDLKSIGSSRGVYDFDVTFGQGAR
                     TLRVDMAGSPVGTPPTIRLQPNGGTFECACENCTTVTLQSTATDAEEDIVSASWFVDG
                     VVSELGGDALSLALPVGAHDVKLVAMDGRGAASFASAPLKVVDTTPPTLTAPPNVVLR
                     SCDYPRTGRASAEDVCSTAWVASTDPGDFMPGVTTVTWYAEDEAGNLSRATQRVTVTQ
                     VEPSACCPPGYNVIRNTGRGPIIGTSGNDCIIGSEGDDVIEGRGGEDYIVALGGQDRV
                     SGGPGNDVILGGEGDDTLDGGDDADQISGGGGQDKINAGAGNDQVHGGRGDDVIRGGV
                     GDDELLGGDGQDTLLGEDDDDILVGGLGDDQLDGAVGNDQLFGGATQDRLQGGVGGDI
                     IVGDDGDDTLEAGPGSDLLAGGKGHNRCSGDGSDRFLSCETRF"
     gene            complement(7439..8065)
                     /locus_tag="VFQ61_26635"
     CDS             complement(7439..8065)
                     /locus_tag="VFQ61_26635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958112.1"
                     /translation="MRLSVDTDIELNEPHCGLGLVTWYRVRVTEKDRRGSFRTVGRLR
                     AARLHLGLAADLNLPWERVFGTSLGELGALCRVFLNPEQDDLRPAFQVALGRDLMFIE
                     SVRMDTTRRGRNIELAAVRRLVDVLGAGCELAILPYASNAEALLWSRLGFQVSTPGEE
                     EGFMHLACGGEQPRTLETDVPGEFQVVPVRRAPAHRNNRACVTRCVSV"
     gene            complement(8210..9118)
                     /locus_tag="VFQ61_26640"
     CDS             complement(8210..9118)
                     /locus_tag="VFQ61_26640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958113.1"
                     /translation="MRRFRKVLALAASAALVWACRGDPRRGSSSGNAGSTHGSGGSTL
                     SLPSGGQDGGEGLGGNTSETPAASGGAAGSAGAADTAAQGGAAGISSASASGAAGELQ
                     GGAESMAAGTTGQATESPEGDAGEPTSPDLRPWKLAFIAPGPFDSDFAAHLGARGVSA
                     HVRADALCQAAAEGAGFKGTYHAWLSTSTAAARDYFAAYAMNGPWHRMDDELVAATLA
                     ELTGKKGLRSPILYGPDGEVGGMAWTGTGPRGELASNCEDFTTADADVGWAAYCATAT
                     AQDYGWSSSSVIACGGTGRGLTCFEQ"
     gene            complement(9115..9414)
                     /locus_tag="VFQ61_26645"
     CDS             complement(9115..9414)
                     /locus_tag="VFQ61_26645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958114.1"
                     /translation="MAIAFDWRVEDEEEVPTIEVSECYDARVVQLAWQSSRMAWLWGG
                     EKLGPHPVRREGFLEASYRCQRHYLVAIHLLSLRSMRGAAAELDRAYELERRYLK"
BASE COUNT         1706 a         3289 c         2905 g         1689 t
ORIGIN      
        1 atcctggaag acgagttcgg cgtccaacgt gttgtggacg aactgctccg atgcttccac
       61 cgccaagcac agagcccaga acaatgggat ctccgcggct ccaccaatac cgaggtggta
      121 ttcggtgccc tccgaaaagg cgagtacatc tcgagagccg agccccagaa agtgtccgtt
      181 gctcggtagc tccgctcccc agtccggcat cctccaaacc tgctcgggcg caaacccttc
      241 tccggtgttg atctgcacgt agagccggga ccgacccggc atcttgagga cacggtccgg
      301 cagaccgtcg cccgtcacgt cggcaaagtc gatcagagtc cgcgtgctcg tatacgcgag
      361 gcctcctcca acgccgccat acccaatctg gtagctattg gcgacgttcg tctggagctg
      421 cagcgccgct tcctcgttgg ctttcgaggt ggtgtcgccc aggccttcgt cgccaatggt
      481 ggatccgaaa cgggccatcg gtagcgcaaa ggggggccca aagctgtagc cgaggttcag
      541 ccgaacttga gcgtctgcgc ccaagttctc gcgctccacg cgatcgggta ggccgtcgcc
      601 gttaatgtcg atgagatcga gcgtggtgcg cgttcgaccg tatgtgcgcc caaacgacgg
      661 caccgcgttg agaatggact gcacggtgcc gctggccccg aggcgttctg aaatgccggc
      721 ggcgcccgag ccgggcttga gcccgtagcg ccgattgctc ccttcgaact tgcgaaatcc
      781 accgccgaag tcccgcggct ggtcggggcc aaagctcccc tgaccgtcgc catactggac
      841 cgagccggtg tcgaacgagt ccgggtaacc atccccgttc agatcgatga gatctacgtc
      901 cgcctccgaa gtggatccgg tgttcgagaa gtccacgacc gttgcggaga ggttgatcgc
      961 gctgcttgac ccgtgactct tccgaaaggc aggggcctga gctgctcgaa cgagggcgag
     1021 agggttggca ccgactcgag agggcttcag ctcacctgcc gcgatgtata gatcgagccc
     1081 gctcgagatc cacgcgggga ggggtgaacc ggcgacgagc tgcccggggt tgggcgcgat
     1141 gccgcgaggc gagacgagcg caggacggaa cggtagatcc tcggctcctt gcttcgtctc
     1201 gtactggcgg cggtcttcga tgactgcgtt ggatagagcg ggactgctgg ctgtcttgtg
     1261 cgtccactgc ccggtactcc actgatggta gccgcccgac atcacgccgc gctcgttcaa
     1321 atcattccat gccgccacgc ggtgcgagtt cagaacgggc gtcgggtagt tgattccgga
     1381 cgactcagaa cggagcgcga cattccagaa catctcctgg ttgcccttgc gaagagccgt
     1441 caagtagatg ggctctccgg cggtcatggt gagccccgtg agcacctgca cgccgctcgc
     1501 gccggcaggc acccgaatct ttccgagcag gcgttgattg ccctgaatca gtagcaccgc
     1561 gtcgtcagct cccgccgccg ccgagctcgc cgcgagtgca tacgaaaccg tcgcttcgaa
     1621 tgtgctcgtc tccggtgccg cccaggcccg ggtggggagg ttctcgctca acgctgcccc
     1681 gtccggcgcc ccgcgaacga cgtacgtcga gtaagttacc ggcgcgggct gctgcagcag
     1741 ccttctcggc agcggccgct cgggagtggg gtcgccatcc atcgtgcagg tatcggaccc
     1801 gtcttctgga cagtgcagag cgccgcacac gggagaagcg ttttcggtag gaggcgtccg
     1861 gcaatactcc tggtaccaaa cctctgggct ccacgcgacg gcgctcggat cgatcgggct
     1921 gtccgagacc acctcgaacc gaagcaagtc agctttgaaa tcagccggag ctccgctcgc
     1981 gtccagggca gccgatgccg tcatcgcgat ggaggtcggg acagcacttc caacatccac
     2041 gcgggtggtt gcggctgctg caaaggtacg cgcataaagc tcggtttcgg ctcccgtaag
     2101 cgagtccacc cgaaccacgc gcaccgtgac gtcatccgcg gttggcgcga tcttctggaa
     2161 gaccccgttc tgaacagata cgttgacggt tccgaggggc gccgtcgtcc aggcttggtt
     2221 tggcggccct gccaaacgga agtctcgagc ttgcgagaag tcgtagactg gagcgcccca
     2281 gggttcgagt tcagagtgag cactcggact cactgccgtg cagaagcccg cctgacacac
     2341 gctctgatag gacagaagcg gatcccagag catctcggta ccggccatgt ctccgagcgc
     2401 ctgggtgcgg aagaacaccg cctggcccgc attgaccgtc agcgtgaggc caccgtcaca
     2461 cgagcccgtg gttccgccgg gcgagcacgc ggtcgcatct gtcgagccga acgcgcgcga
     2521 ccaaagtggc gagccgtccc catcgcgcca gatctccgcc actacgtccc ctccgcccgc
     2581 ggtcttgcgc gtaatcgcgc cggacagagc cacggtgccg gtgtaaggag caacccatcg
     2641 aacgagcgga tcggcgtagg gtgcactctc ttcgatgctc ctgcgcagag catccggcac
     2701 aacactgctt ctaccggtca gtgtgcgccc gagcggcgcg agcccgcccc gcccgtcgtt
     2761 tcggtagatc aggcccagga agggcgaaac ccaatccacg aagccgtcgc catcgacgtc
     2821 cgcgaagcca ccctgttgct tactgttggt ccaggcccag ttcccgccag cctcgccgcg
     2881 gtcgcccgct acgtgaaccc cgacgttcag gctgcgcgtg ctctgctcgg tttcccagaa
     2941 tgtgtcttcg agtcccgaaa tcgaggcggc tcggtatccg gtcacctccg aggccccacc
     3001 ccatagcgct cggccgccgc ttagaaaatc gggcagcccg tcgccgttga agtcgaggaa
     3061 agtccgtgac gtcctgtcat tgccgccggt gagtccgccg ccaaccccgg cgtggaacgc
     3121 tccgcaaata ccgagcccaa gaaagccgtt cgtcccgcct tgctcgccgg agctcgcgtt
     3181 cagcacgtct ttatcggacg ccgtgcccca gggaagtgca cccgagaacc cgggctgtat
     3241 cgaagggttt cgctcgaagt aggtgaacgc tgtcttgtgc ctgcgagacc accggaattg
     3301 gagctcgccg ttcatgtccg gtgcctgcag ttcgatcgct tcgaggagcg acttggaaaa
     3361 ctcaccggtc ttgtaggtga aggcgtagcg cctcaggagc ggaccgattg ccgcggaggt
     3421 ttcaggtgcg ggaggccgct tcccgggcga gtagaatacg tccatcgcct cgagccgccg
     3481 ggcggtacgc accaggaagc ccgaccgccc cgagctggaa acgtccggtc gttgccctgc
     3541 cgcgccgagc acgaactgca cgtacatcgc cccggcttcg ctgccgtgat cggtgtagcg
     3601 aatctctttc gggtacatct gcacccactt ctggccgccc gctcctaggg tgccgctgtc
     3661 gagcaggtac tcatagtgca cacggttccc gtacgtgtct tcgacttcgg atagacccca
     3721 ctcggcaatc cgccccgtat cggagtcgct gagacggctc gccgcatcca caccgtagcg
     3781 gaagcgcgtg ccgttcttgt cggtgagctc ccaccagtaa gcgttcgggc ctgcggtggg
     3841 cgttgagcca tgccgcacga tgcgagcgaa gctcccctcc gcgcggcgca cgaattcacg
     3901 ctcggcttcc cgtggcgctg ggtgcagctg gcgctctcgg ggcccggagg tagcgccgcg
     3961 gggcgtgagc tcgctgccgt cgagcgcgta gagctcggtc tccaaagagc tgtcgtaacg
     4021 gggaactcca aacctcgaat cgatgcggac gcgcgagagg ccgatgtccc agccgaggcc
     4081 catccaaccg ttgcccgcgt cactgctgta gctcagctga agatctggcc caagtccgcg
     4141 ccgaccgggc ggaagcgtga acttgtaaga cacgtgcgcc gcgccgtcac ttccgccggt
     4201 aggcgtcgtc atcggttcga gacccgtcgc cggatctccg agcttcagat cggcaatggt
     4261 gttggtctca tgtgaggcgg tcgtcgggtg gtccgggacc gccagcgtcg cattgatgaa
     4321 gtcggtaaag tgttctgtct ccgcaaccac aagcccttcc gcttcacgca cctcgcgtac
     4381 gcggatcggc cgccagctcc cgctctccgc gtcgaagaag aacgtgccca gatcttgggc
     4441 cgtcattccc gccggcatga gcgttctatt gaacggcaga atcagcgtca ccgggcggag
     4501 aaagcgaagg ccatgcggcc cgagccgaaa cgcccgctgc ccgggggtga cgtttgccat
     4561 gcccgtatcg agcgcccccg cttctcggtc cgcgaccgtg cgcatcgtca ggcgttcttc
     4621 gtgcgcgacc gcgccggggg gcacgatcag cgtcgcccct tcgaaacgca gtgtgctctc
     4681 acgatcggga taggcgacct cggaataggt cgcttcccga tcttccacgg ccggccgcgc
     4741 cggcagaagc gcggggctcg gggcggcgtt cgcatcgcaa gtattcagtg ctccccgccc
     4801 acgaaacgcc tcgaacaaga gatcggcact accgccagga actcgcgatg cgctctgctc
     4861 acgactcacg ccgagagcct tagagacgag cgcctcgaac gggtcgggcg tgcggctcac
     4921 cggactgatg agcttcgtga gtggagtcgc cttggttcgc gactcacact cggcagaacg
     4981 gctctgatcg gcgctcgcga tcgtctttgt cgactgcgga tccaagctcg cgagctgggg
     5041 cagcgctccc aagtcccgag accgtgctga cgccctcgct acggcccagc tcgccgcgaa
     5101 tgcgggctga acgctcgaga aacacattaa caataggcac acggcattcg tcgcgcgcag
     5161 ccaaagcgcg tatggatatc gcgagttgtt cataaaaaaa ctctcccgtt tcccctacag
     5221 tccgaactca gaagcgtgtc tcacagctca agaagcgatc ggaaccgtcg cccgaacacc
     5281 gattgtgccc cttgccaccc gccagcaagt cgctccctgg acccgcctcg agtgtgtcgt
     5341 ctccgtcgtc gccgacgata atatcaccac ccacgccgcc ctgaagtcga tcttgcgtgg
     5401 ctcccccgaa gagctgatcg ttcccgacag ccccgtctag ctggtcgtcc ccgagtccgc
     5461 caaccaggat gtcatcatcg tcttcaccta gcagtgtatc ttggccatct ccgcctagca
     5521 gttcatcgtc accgactccg ccgcggatca cgtcatcccc acggccacca tgtacttggt
     5581 cattgccagc gccagcattg atcttgtctt gccccccgcc gcccgagatc tgatccgcgt
     5641 cgtcgccacc gtcgagcgta tcatcgccct ctccgcccaa gatcacatcg ttgccaggac
     5701 ctcccgacac gcgatcttgc ccgccgagcg ccacgatgta gtcctcgccg ccgcgccctt
     5761 cgatgacgtc gtcgccctcg ctgccaatga tgcagtcgtt accggaggtt ccaataatgg
     5821 gtcctctgcc ggtgttccgg atcacgttgt agccgggagg gcaacaagcg gacggttcca
     5881 cctgagtcac tgtcacccgc tgcgtcgcgc gggatagatt gccggcttcg tcttccgcgt
     5941 accaggttac cgtggtgact ccgggcatga agtcccccgg atccgtgctc gccacccatg
     6001 ccgtcgagca cacgtcttcc gctgaggctc tgccggtccg aggatagtcg caacttctga
     6061 gcaccacatt gggcggcgct gtcagggtgg ggggcgtcgt atcgaccacc ttcaacggcg
     6121 cactggcgaa ggacgcagcc ccgcgaccat ccatggcgac gagcttcacg tcgtgggctc
     6181 cgaccggaag agccaagctc aacgcatcgc ccccgagctc agagaccact ccatcgacga
     6241 accaggaagc gctcacgatg tcttcctcgg cgtcagtagc cgtcgactga agcgtgaccg
     6301 tcgtgcagtt ttcgcacgcg cactcgaacg tgcctccgtt cggctgaagc cgaatggtcg
     6361 gcggcgttcc gacgggtgaa cccgccatgt cgacgcgcaa ggtacgcgcc ccttgcccga
     6421 aggtgacatc gaagtcgtac acaccacggc tggagccgat agacttcagg tcgtggtcgt
     6481 tcgaggcgtg gacgctcgcg cgctcgccgt ccagcgtgaa gttcccttcg acccggaccg
     6541 agccgcttgg caagaggaac tccggcccac tctgacgccc ggcgctccgc ccttgcatct
     6601 gaacctgcgc ggcggtcacc agtgcgccgc ccagttcaaa cggctctgcc gccacggcat
     6661 cgaacctcga gacgtccacc gcgcacgact gccccgcgcc ttggcaaggc gcgtaggtca
     6721 cgtccaccac gccagcgccc gggaccgaag cttgggcacc gtccaggttc aggtgcagcg
     6781 ttcccgcgat ggcggtacga aagtcggctg ggccgccgct gaagtagtcg gcgttgagat
     6841 tcgccggcaa gcgggtcaga tcggcaccca gtcgcggatc ctcgcatggc gtcacaaaga
     6901 acgtctcgca gctgagcggg agggccgctt cggggtgcgt tcgaaagtat cgcgcaaacg
     6961 catccccgca gcttcgatcg cacgctgcta tcacttccgc ttcgctcgct gcttggcgcg
     7021 cgtacgtcac gaactgaagt cccgggtcgt ccggcgagca cccaccgtaa tagagcggcg
     7081 actgttcgtg gcgacccggc gccgtttcat gcacgaggcc aaagcatgtc cagatctgcc
     7141 cttcatcacg aggcagcccg tcgcacgcgg cgacacccaa cgcgagcgct agcccaaaca
     7201 tgcgaacttt tcgaacgtga aaaacggaca taccccagac ctcccgaagc gatttgccag
     7261 gtgtcacaac acgcgaatga acacgggggc gcagcgcttg ccgcacgccc aaccattcga
     7321 ctgagaagcg cgatagttat tctaagccaa gcaagatcaa cacgaggaca acaccgaaca
     7381 cacactcaac acgttgcaca gccgagcgct tgggcgctca gaagatcaat ccctcgcgct
     7441 acaccgacac acaccgggtt acgcaagcgc ggttgttgcg gtgagcaggg gcgcgacgga
     7501 ccggaacgac ctgaaactcg ccaggcacgt ccgtctcaag cgtgcggggc tgctcgccgc
     7561 cacacgcaag gtgcatgaaa ccctcttcct cgcctggcgt gctcacctga aacccaagcc
     7621 ggctccacaa gagagcctcc gcgttgctgg cgtagggtaa aatggctagc tcgcatcccg
     7681 cgccgagcac atcgaccaag cgtcttaccg ccgcgagctc gatgttcctt ccccgccgcg
     7741 tcgtgtccat gcgaacgctc tcgatgaaca tgagatcacg cccgagcgcc acctggaacg
     7801 cggggcgcaa atcatcttgc tcggggttga ggaagacgcg gcacagcgca cccagttcgc
     7861 cgagcgaggt gccaaacaca cgctcccaag gcaagttcag atcggccgcg agccccagat
     7921 ggagccgcgc cgcgcgcagc cgccccacag ttcgaaagga accgcgacga tctttctcgg
     7981 tcactcgcac ccgataccaa gtcactagcc cgagcccgca gtgcggttcg ttcagctcga
     8041 tgtccgtatc tacactcaga cgcatgatca gctccgtcgt atgtctcgag gaaggaaagt
     8101 tcaggggccg cgacgatacg cggggttgag gcgtgaccca cgggagggca ttgggtcggg
     8161 tcgtccccac atacagctat tgtcgcgacc ccctggttag atcgtgattt cattgctcga
     8221 agcaggtgag ccctcgaccc gtgccgccgc aggcgatcac cgaggagctc gaccacccat
     8281 agtcttgcgc ggtggcggtc gcgcagtacg cggcccagcc cacatccgca tccgcggtcg
     8341 tgaagtcttc acagttgctc gcgagctcgc cgcgaggccc tgttccggtc caggccatgc
     8401 cgcccacctc tccatccggc ccgtacagaa tcggcgaacg taggcccttc ttaccggtga
     8461 gctctgccag agttgcagcc accagctcgt catccattcg gtgccaggga ccgttcatcg
     8521 cgtacgcggc gaagtagtcg cgagccgccg cggtgctcgt agaaagccaa gcgtggtacg
     8581 tacccttgaa ccccgccccc tccgccgctg cctggcatag cgcgtctgcc cgaacatgag
     8641 cactaacccc gcgcgcgccg aggtgcgcgg caaagtctga atcgaatggt cccggcgcaa
     8701 tgaaggcaag cttccagggt cgcagatccg gcgaggtcgg ttcacccgcg tcgccttccg
     8761 ggctctccgt ggcttgtccg gtcgtgcccg cggccatgct ttccgcaccg ccctgcagct
     8821 cgcccgcggc gccgctggca ctggcgctcg atatgcctgc ggcgccgccc tgggccgccg
     8881 tgtccgcggc tcctgcgctg cccgcagcgc cgccgcttgc agccggagtc tctgaggtgt
     8941 tgccgcccag accttcgccc ccatcctgcc cacctgatgg gaggctaagc gtactaccgc
     9001 cggagccatg cgtgctgccc gcgttgccgg aggatgagcc ccggcgtggg tctccgcggc
     9061 atgcccaaac gagagccgcg ctcgcagcga gcgccagcac cttacgaaac cgtctcattt
     9121 gagatagcgc ctctcgagtt cataggcccg gtccaattcc gccgcagcac cccgcatgct
     9181 tctcaagctg agtaggtgaa tcgccaccag gtagtggcgc tgacaacggt acgaggcctc
     9241 aagaaaccct tcgcgccgca ctgggtgagg gccgagcttt tcgccgcccc acagccaagc
     9301 catccgggag ctctgccagg cgagttgaac gacgcgggcg tcgtagcact cgctcacctc
     9361 tatggtgggc acttcctcct catcctccac acgccaatcg aatgcgatag ccattctcca
     9421 ccctcgtgtt ccgtctatgg ctccaggagc cccattgttt cgatgagttc atgccgcgtc
     9481 gtgaaccagg ccgcgcactg ctgcccgatc tgaagcgcga tgcgatgcct ttcccgccga
     9541 tcgaggacgc gtgcgttctc gtagatcgcc aggcgtagct cgtccgcgg
//
LOCUS       DASTKU010000765        14983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1713035, whole genome shotgun sequence.
ACCESSION   DASTKU010000765 DASTKU010000000
VERSION     DASTKU010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14983
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713035"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VFQ61_26650"
     CDS             <1..281
                     /locus_tag="VFQ61_26650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958115.1"
                     /translation="PSAEILPNPSVAPDRERVVQGGMIGWSVWVARIIQFADGTKKEE
                     RRKVTYRPKVRRVEVHPCRVPEGEKGYTGERCPEPEEEEAQAATESER"
     gene            complement(304..1518)
                     /locus_tag="VFQ61_26655"
     CDS             complement(304..1518)
                     /locus_tag="VFQ61_26655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958116.1"
                     /translation="MRELRNLVLAGCVLALGGFAPACSNDDDSPTPASTSGRGGTSSA
                     GTDRGGSAGQTDSSEGGQGERGGQSGTSEGGTANSASGGAEGNQGGEDNHAGATNSGG
                     AAGGPSTAPPVSIFYLDASRDRVMWLPPRARQPKILVSNAGKGPDGIAVDTTIGALFW
                     TTMGVPADEDGAVSRANLDGSESTSIIATGDTFTPKQIQVDASGGKLYWADREGMRIQ
                     RADLDGSALETLVTVASGAAARSDASHFCVGIAIDPEGGYLYWTQKGDATASNGSIRR
                     AHLEIPQGQTDIDRTDVEILFEGVSEPIDLAIDPVAGYLYWTSRGDNTVNRAGLEVPD
                     GETAATRRDREVLVPNVEEPIGISLGPTGENLYFTNLAGTIGRYDLNTGTYEDVLTGG
                     GALTGLSVVAPF"
     gene            complement(1582..2877)
                     /gene="manA"
                     /locus_tag="VFQ61_26660"
     CDS             complement(1582..2877)
                     /gene="manA"
                     /locus_tag="VFQ61_26660"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET9958117.1"
                     /translation="MDDASSDRGPPGALSHEMYELSALDNAVQNYAWGSRSFLAEFLG
                     RSVPSAKPEAELWMGAHPLAPSKLLTRAAAQDLRDLIATAPEAMLGPRVLNRFGPELP
                     YLMKVLAAAEPLSLQVHPSAAQAEAGFIREEGLGIPRTDPKRNYKDRRHKPELIVALT
                     PFYALCGFRPIAHTRQLFAELGSPALEPYARALCTSDARTALRSSFSRLFDNAADTKK
                     TLARSVETAIRAATTRPTSFRFELEWARRIADLYPGDVGLIAALYLNVVRLEPGQALY
                     LPAGNLHAYLEGAGLEIMASSDNVLRGGLTPKHVDMSELLEVLDFSELRVQRIEPLAR
                     EGEAVYPSPAAEFRLSRRDVEGAQQVAYSAAGEPREQGRGNDCGPEVWLATQGEITLS
                     SCGGRQIALRAGQSAFAPACDPVDQVAGHGTAFIARVPN"
     gene            complement(2835..3947)
                     /locus_tag="VFQ61_26665"
     CDS             complement(2835..3947)
                     /locus_tag="VFQ61_26665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958118.1"
                     /translation="MRANSVRLKGAWAGGAWRKSSFLEGKSAWLKGQSTWLKAAAGSF
                     ALAWACAPKPAPSEPRTETALPQDGNARPPAARSSSQRQGSTAPPPIVPNTNLLPGSA
                     APRPCGELGCVEFSSAEAALTDLLRTEPRVLALGEIHAPKSQTAVPSTATRFGELLPL
                     FAGRSKDLVIELLLSSGQCGAVEKQVETKTAPIREPQAASNQNEFVELGFRAKALGIQ
                     PRALTVACKDYARVAGAGSSDIDQLLALIGARVEEEVTALLPKRSTGTILVYGGALHN
                     DLHPRPGRERWSFGPSLAQATNGAYAELDLVVPEYVADNDTFRAQVWYSRLPARPLAD
                     APPEPPVFRLLQPAPRSYVLIFPWTTPHPTAARPAP"
     gene            complement(4016..4951)
                     /locus_tag="VFQ61_26670"
     CDS             complement(4016..4951)
                     /locus_tag="VFQ61_26670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958119.1"
                     /translation="MTSSPDGGSIRDLRRKGRVRRHDFLRALCLALTCAAVLANVQPA
                     GADLANATQRGPEDLSAQVQRSIEAIGRGAFDEGVSGLELLADRGVVHADVSLTRAYA
                     YIERARSRSARPGDLGRAVAALEECEWLRPGTCQAGSSLSTLRSEIARRRARSGATEV
                     AERPALARAVSQILPENTWAWLALCGSSLVSLGLAVYLFVKKRTAEITGSVAIGAGLL
                     LMCVTAPIALMARHYRQTSRPAVVVAPEARLLEESGRPLPVRAKAADAVPEGALVYVR
                     DQHDGRSEVEWGRISAWVDSSQLRVLQAPSDNAGR"
     gene            complement(4948..6891)
                     /locus_tag="VFQ61_26675"
     CDS             complement(4948..6891)
                     /locus_tag="VFQ61_26675"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HET9958120.1"
                     /translation="MIRLAGPHVRLMSRARPYSLALGISAGLGTIFSVNAAHAELVLR
                     TQASSRSVEVGEVFSIELVIRGDEDVEPANAKLPVPSGLQVSGPHTSTQTQVSITNGR
                     MQRINGLTLSWSLVANQAGNFRVGPPSVDLNGKHSVGQAITVEVTPAGTARRSPPGGR
                     RPSGFPFPLDPFDPFSAFRGQGIPGFPFDDLDAPDIPEPAAPPEYQLDHAPDQTAFLI
                     AKATPRSVVLGEAVHLKIYSYGGAGPYEEATSNEPSHEGFLSYSVESDGRTAVPVRIG
                     ERSYIAARIRDVLLFPIQTGELRIGAMRFGFGGRGYRAPRGSGGLIRESLPIDIKVVE
                     PPLAGRPAGYRLGDVGQYSLKASVEPRKVRQGEAVAVIAKLEGTGNIPSKLALPQQTG
                     VDWMEPTVVEKLDTTGGKLTGSRTFTYVVRAEKAGTIDLGELTLPFYDAEQRRYEVAR
                     QPLGTIEVAADPNAPAPVAGSGKEDRLAKLVEPPRQLGLASTRGPFISDRPGFFGLLL
                     SAPLLVLAGSGLLRVAQRARERWQARDVSPERRAQRELEAARAAAASSDLAGTAAAVE
                     RALHLALEGGTQVRSRGLLRSELAPALERRGLSRGLAEEVVELLQATENARFVQGGSG
                     EATQQLLARGEALVGRLIKGGRT"
     gene            complement(6888..8957)
                     /locus_tag="VFQ61_26680"
     CDS             complement(6888..8957)
                     /locus_tag="VFQ61_26680"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9958121.1"
                     /translation="MRFAAPYWLFGSALALAVAVLLIAGGFLLMRATRRFGDEALVLS
                     LVTGRPGGRRALKGGLLVTAVALAFLALAQPQYGRGTRMVPATNLDVVVVLDFSKSMY
                     ARDIAPSRTLRAKSEVSRLIADLPGARFGAVAFAGQPMAFPLTSDGGAISQFFRQLAP
                     NDMPVGGTAIARALEAGRDLFARDPLSKQHKKVMLLVTDGEDLEGDPVAVAQNAAGEE
                     ISIDVVQVGGRTPEPIPEVNESGQVKGYRQDAEGKPLTTSLSAAGEEQLTKIASVGGG
                     QIVRSAGGKTGIEVIAARLRKLMTEELSERVETVYADVFFYPLGLAALLLLLEVFVNE
                     TAPRARPAVLPPPEPHKRRRRRAKVAGITTLPGLLLVAGLSSSCAQAEKTLFERAAPD
                     VETAIEKLKANDGAAAAALLEQYLSTGKCEKGELGTPERIRQRENASFDLGLALFRVG
                     EQYGQRFGEEVTTPDGEPTPEQKALAEQRSGEVECALRVVRVVAGNTDAAPDFRAEAS
                     YLAGNLEFLRGDYRAAVKSYDAALKIAPGIETAPAPRKSANSQSAPTANESVGLNAAY
                     NRSIALRRIEEEEKKKQEKEKDKEKDQKNDDEKKDQDKKDQDQKDDEKKDQAQKDAKN
                     DDKQAQQDKQEPPSQGQDENQAQPPDSNVSQDEAILDQLEQAPTVQQQDAKNRALKAR
                     SYGMEDK"
     gene            complement(8954..10111)
                     /locus_tag="VFQ61_26685"
     CDS             complement(8954..10111)
                     /locus_tag="VFQ61_26685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9958122.1"
                     /translation="MKHRPRLMRALTVFGFTLLALLFGFAYPALTRGTDFFDANYEQR
                     WFLLGLVLVPLVFWRGIWGEDRRMPRLKLGTLSALAVGPSGIRVWLRDLPGVLRSVAL
                     ILFVLAMARPVDTLRPQTTDEEGIDIMLALDMSGSMQAALSNVPPDLARYIPEKPRGI
                     RPTRLDGAKAVMRDFIARRKSDRLGVVVFGSSAYVLSPPTLDYHLLDVLVSKMELGLV
                     DANGTAIGDAVGVAVARLRKSSAKSKAVILLTDGDNKGGKLAPEYAAHLANTVGAKLF
                     TVQIGQGDTAEVQDGFDLFGQPRYVQRSFPVNPKLLQTLAQKTSGAAYVATDVKSLQA
                     SFHDVLNRLEKTKLEASIAHYEDLFGYFLLPGVLLLSLEALLRSLVLRRFP"
     gene            complement(10108..11361)
                     /locus_tag="VFQ61_26690"
     CDS             complement(10108..11361)
                     /locus_tag="VFQ61_26690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958123.1"
                     /translation="MARWRVGLALLVHVASSNAAQAQGAVTTGAQAPPGAASAAAGSS
                     AVSAPVPAASPTHTQSTGTCVERLPDGKARPKLTEKFPAHGVTGHALTLELVVEHGPG
                     ETVLPGGFKLDAGGDDYKALEGAHFFIPDPRGPAKPTLTRSEEGGRARTTVQLQLVPL
                     PDKPGRQELTLPALPIAISRASGEMMTLCTSSHALIVDDPIANDPNPKPKPNPAPRRQ
                     FEEWTLAKHVTYAGLIALALGALLALIISRWMRRPKKVAPPPPPRPPWEVALEGLFDV
                     GHSGLLREQRYGEFYERVSDIIRQYLGDRYGYEGLESTTREALAALRRVSVPFDVWVK
                     VQEFMQDADLVKFARNDPTEAQCASALTRAEALVALTRPALPLGAPQPGSADGTAADG
                     ASGTATDNGITGTEKPDPAPPKEAP"
     gene            complement(11361..12239)
                     /locus_tag="VFQ61_26695"
     CDS             complement(11361..12239)
                     /locus_tag="VFQ61_26695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9958124.1"
                     /translation="MIPKELLKALRKIEITTNRLATEQLSGNYTSVFKGQGLAFREVR
                     EYQPGDDVRSIDWNVSARMNNTYVKVFVEEREMTVMLVVDLSQSQQFGTRTANKSRLA
                     AEVAALCAFSAIKHNDRVGLILSTDQIEKIVPPMKGQKHVMRVVREILGATPERPGTD
                     LKVALETLYRVARRRSVAFVLSDFFAQGHERALSLAAARHDVIPVMLVDPRDEELPDV
                     GLAAFEDLETGESVLVDTSSKRVRAHYAKQMRAQREGQVKLFRKLGLDHVVVRTDQPY
                     IRPLRDLFARRARRSR"
     gene            complement(12287..13273)
                     /locus_tag="VFQ61_26700"
     CDS             complement(12287..13273)
                     /locus_tag="VFQ61_26700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9958125.1"
                     /translation="MQDVRALNEIVARESAFIDDLTAEIGKVIVGQTYMIERVLIGLL
                     AGGHVLLEGVPGLAKTLTVRTICEAVAAKFSRIQFTPDLLPADLIGTVIYDQKTGEFT
                     SKLGPVFANLVLADEINRAPAKVQSALLEAMQEHQVTIGDRTYALPSPFVVMATQNPI
                     EQEGTYPLPEAQVDRFLLMIKVSYPTREEERAVMDRMTQTRPASISPRTTPERLLEAR
                     KVVRDVYMDDRVKDYIVDVVFATREPSAKGMKDLAPLLEYGASPRASIALNLAARAHA
                     FLRHRGYVTPEDVKAVGPDVLRHRMVLSYEAEAEEVTAEDIVRRVFEVVEVP"
     gene            13762..14739
                     /locus_tag="VFQ61_26705"
     CDS             13762..14739
                     /locus_tag="VFQ61_26705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958126.1"
                     /translation="MSTNNPSKNGPSSNETSQASAGKTGKRGRRTLRGLRGALFSSVL
                     FVGLARSSHAQTYTPTDTAREVQLRSALLRNSFESVSVPLRRYAPALLGSAFLVGAAG
                     FAFDPPPTGKFEEKRWHLTAGLGTFAALSFGTYALPTRYQGTTFALLAGAGLAGAGAV
                     GWAWSEQDTRSARVVFGSMLGAGILTLGLEALDTALLTPLDQSEIEGHLRRLGRRGAA
                     PSRAELRRMEWELSLTRRRIPAWLVPLTVTLSGVVAASPLLAFPDASKSDRVLSALVS
                     IQLLQIGNAGLISARSRDTGYPAYVRALRNVRLAPLAGGGANVSVSSTW"
     gene            complement(14772..>14983)
                     /locus_tag="VFQ61_26710"
     CDS             complement(14772..>14983)
                     /locus_tag="VFQ61_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HET9958127.1"
                     /translation="IRDDRITFYYSRDGKLWTAAGWEYDASILSDEHAVPCGFTGNFV
                     GMACQDLSGSRNHADFDWFEYREID"
BASE COUNT         2542 a         4989 c         4719 g         2733 t
ORIGIN      
        1 aaccgagcgc ggagattttg ccgaatccaa gcgtcgcacc ggatcgcgag cgggtggtgc
       61 aagggggcat gattggctgg tccgtatggg tggcgcgtat cattcagttc gcggatggaa
      121 ccaagaagga ggagcgacgc aaggtgactt atcggcccaa ggtgcgtcgc gtggaggtcc
      181 acccctgccg agttcccgag ggcgagaagg gctacaccgg cgagcgatgt ccagaacccg
      241 aggaagagga agcgcaagcc gccaccgaat ccgagcgctg acgaatgaag tcgagagcga
      301 ccttcaaaac ggcgccacga cgctgaggcc ggtgagcgcg ccgccgccgg tgagcacgtc
      361 ttcgtaggta ccggtgttca ggtcgtagcg gccgatggtt cccgccaagt tcgtgaaata
      421 gagattctct ccggttgggc ccaggctaat gccaatgggc tcctctacat tcggcaccag
      481 cacctcgcga tcgcgccgcg tggccgccgt ttccccgtcg gggacctcga ggccggcgcg
      541 attcacggtg ttgtcgcctc ggctcgtcca atacaggtaa ccggcgaccg gatcgatcgc
      601 gagatcgatt ggctccgaga cgccttcgaa caggatctcc acgtccgtgc ggtcgatgtc
      661 agtttggccc tgagggatct ccaagtgcgc acgacgaatc gagccgttcg aggccgtcgc
      721 atcacccttt tgggtccaat acagatatcc gccttcggga tcgatggcga tgccaacgca
      781 gaagtggctc gcgtcacttc gagccgcagc cccgctcgcg accgtgacca gcgtctcgag
      841 cgcggagcca tcgagatctg cacgctgaat gcgcatgccc tcacgatcgg cccagtacag
      901 cttgcctccg ctcgcgtcga cttggatttg tttgggcgtg aacgtgtcgc ccgtcgcgat
      961 aatcgacgtc gactcactgc cgtccaaatt ggcgcgcgag acggctccgt cctcgtcggc
     1021 gggaaccccc atggtcgtcc agaacagcgc tccaatcgtg gtatccacgg cgatcccgtc
     1081 cggcccttta cccgcgttgc tgaccaagat cttgggttgg cgagcgcggg gcggtaacca
     1141 catcacccgg tcgcggctcg cgtcgagata gaagatagaa accggtgggg ccgtgctcgg
     1201 accccccgcg gcgccgccgg agttggtcgc gcccgcgtgg ttgtcttcgc ctccttggtt
     1261 gccctctgct ccgccgcttg cgctgttcgc cgttccgcct tcggaagtgc cgctttgtcc
     1321 gccgcgctcg ccttgtccgc cttcgctcga gtccgtctgt ccggcgcttc cgccgcggtc
     1381 ggttcccgcg ctgctcgtgc cgccgcgtcc gctcgtgctc gcaggcgtcg ggctgtcatc
     1441 gtcattcgag caggctggag cgaaaccacc gagcgctagg acgcatcctg ccaagaccaa
     1501 gttacggagc tcgcgcatgc aacagaacta accgcgaagc cgaaccaagg taaagggtgg
     1561 gacttaggcc ttcatcggga ctcagttggg gacgcgcgca atgaaggccg tgccgtgacc
     1621 cgccacctga tcgaccggat cacaagcggg tgcgaaagcg gactggcccg cgcgcaatgc
     1681 aatctgccgc ccgccgcacg agctgagcgt aatttctccc tgcgtggcga gccacacttc
     1741 ggggccacag tcgtttccgc ggccctgctc gcgaggctcg cccgcggcgc tgtatgccac
     1801 ctgttgcgcg ccctccacgt ctcgacgact caagcgaaac tcggcggcgg gcgagggata
     1861 taccgcctcg ccttcgcgtg cgagcggttc tatgcgctgc acgcgaagct cactgaagtc
     1921 gagcacctcc aagagctcgg acatgtcgac gtgcttgggc gtcaacccgc ctcgaagcac
     1981 attgtccgaa ctcgccatga tttcgagccc cgcgccctcg aggtaagcgt gcagattgcc
     2041 ggcgggaaga tagagcgctt gcccaggttc caggcggact acgttcaaat acagcgccgc
     2101 gatcaagccc acgtctcccg gatacagatc ggcgatgcgg cgggcccact cgagctcgaa
     2161 ccgaaacgag gtgggtcgcg tcgtcgccgc gcggatcgcc gtctctacgc tgcgcgcgag
     2221 cgtcttcttg gtgtcggcag cattgtcgaa cagccgggaa aagctggagc gaagtgcggt
     2281 ccgcgcgtcg ctcgtacaca acgcccgcgc atagggctcg agcgccggtg agccgagctc
     2341 tgcaaataac tggcgcgtgt gcgcgatcgg acgaaagccg cacaaggcgt aaaacggagt
     2401 cagcgccacg atcagctccg gcttgtggcg ccggtccttg tagtttcgct tgggatcggt
     2461 gcgagggatc ccgagccctt cctcgcgaat gaaacccgct tcggcctggg ccgcgctcgg
     2521 gtgtacttgc aaagagagcg gttcggcagc cgcgagcacc ttcatcaggt acgggagctc
     2581 ggggccgaag cgattcagca cgcgaggccc caacatcgcc tctggtgcgg tggcgatgag
     2641 atcgcgcaaa tcctgggcgg cggctcgcgt gagcagcttg gagggcgcga gcggatgggc
     2701 gcccatccac aactccgctt cgggcttggc gctcggtaca ctgcgaccca ggaactccgc
     2761 caggaagctg cgcgatcccc aggcgtaatt ctgaacggcg ttatctaagg cactcagttc
     2821 gtacatctcg tggctcaggg cgccgggcgg gccgcggtcg gatgaggcgt cgtccaaggg
     2881 aaaatcaaaa cgtaggagcg tggagcgggt tggagcaagc gaaacacggg aggttcgggg
     2941 ggcgcgtctg cgagtggccg cgctgggagc cgggaatacc agacctgcgc gcggaaggtg
     3001 tcgttgtcag ccacatactc agggacgacc aaatcgagct cggcatacgc cccattggta
     3061 gcctgcgcca ggctgggccc aaaggaccaa cgttcacgac ctggccgggg atgcaggtca
     3121 ttgtgcagtg cgccgccata aaccaaaatc gtacccgtac tgcgtttcgg gagcaacgcg
     3181 gtgacctcct cctccacacg cgcgccgatg agagccagca attgatcgat atccgagctg
     3241 cccgctccgg cgacccgggc gtagtcttta cacgctaccg tcaatgctcg gggttggatc
     3301 cccagcgctt tggcgcggaa gccgagctcc acgaactcat tttggttcga ggcggcttgg
     3361 ggctctcgaa tcggagcggt cttggtctcg acctgctttt cgacggcgcc gcactgacca
     3421 ctcgagagaa gtagctcgat caccaagtct ttggagcgtc cagcgaacag cgggagtagc
     3481 tcgccaaagc gggtcgcggt cgaaggtact gccgtttggc tctttggcgc gtgaatttcc
     3541 ccaagggcga gcacgcgggg ctcggttcgc agcagatccg ttagcgcggc ttcggccgag
     3601 gagaactcga cgcacccgag ttctccgcaa gggcgcggcg cggccgagcc tggcagcagg
     3661 ttcgtgttgg gtacgatcgg cggaggtgcc gtcgagcctt gtcgttgcga ggaagaccga
     3721 gcggcaggcg gccgtgcgtt accgtcttgg ggcaacgcgg tttcggttcg cggctcactc
     3781 ggggccggtt tcggcgcgca cgcccacgcc aacgcaaagg acccagccgc tgcttttagc
     3841 caggtgctct ggccctttag ccatgcgctc ttgccttcta ggaatgagct cttgcgccat
     3901 gcgccgcccg cccaagcgcc cttcaagcgt acgctgtttg cccgcatgcg atcgagctta
     3961 tcatcggcgc acggccgcgc tcgtgccacg aactcgcgga gaaatgttgg aactctcagc
     4021 ggcccgcgtt gtcggacggg gcttgcaaaa cccttagctg tgacgagtcc acccaggccg
     4081 agattcgccc ccactcgact tcggagcgcc cgtcgtgctg atcgcgcaca tagacgagtg
     4141 ctccctcagg aacggcatcg gcggccttcg cgcgcacggg taaggggcgg cccgactcct
     4201 ccagcagacg cgcctctggc gcaaccacca ccgcgggccg cgaggtttgc cgatagtgac
     4261 gggccatcag cgcgataggc gccgttacgc acatgagcag taagcctgcg ccgatcgcga
     4321 cggaccccgt aatctccgcc gtacgtttct tcacgaacag gtaaacggcc aacccaagcg
     4381 agaccaagct cgaaccgcac aacgcgagcc acgcccaggt gttctcgggc aatatctggg
     4441 acaccgctct cgcgagcgcc ggtcgctccg ccacctcggt ggccccactc cgcgctctgc
     4501 gccgcgcgat ttcggagcgc aaggtgctca gtgaagagcc ggcttggcag gtccccgggc
     4561 gaagccactc gcattcttcg agcgccgcga cggctcgacc gagatctccg gggcgagccg
     4621 atcgcgaacg cgcccgctcg atgtaggcgt acgctcgcgt caggctgacg tccgcgtgaa
     4681 ccacgccgcg atccgcgagt agctccagcc ccgagacgcc ctcgtcgaac gcgccgcgtc
     4741 cgatcgcctc gatcgagcgc tgcacttgag ctgacagatc ttcgggtccg cgttgggtcg
     4801 cattcgccaa gtcggcgccg gcaggttgga cgttcgcgag cacggccgcg caggtcagcg
     4861 ccaaacaaag ggcccgcaaa aagtcgtggc gccggaccct tcccttgcgc cgcagatcgc
     4921 ggatcgagcc accgtcagga gagctcgtca cgtgcggccc ccttttatca atcgacccac
     4981 gagcgcttcc cctcgggcga gcagctgctg ggtggcttcg ccgctccctc cctgaacgaa
     5041 acgcgcgttt tcggtggcct gtagcagctc cactacttct tccgccaacc cgcgggagag
     5101 gccgcgccgt tcgagtgcag gagcaagttc actgcgcaaa aggccgcgcg agcgcacctg
     5161 ggttccacct tccagcgcca agtgcagcgc ccgctctacg gcggcggcgg tgccagccag
     5221 atcactgcta gccgccgcgg cccgcgccgc ctcgagttca cgttgagcgc gccgctccgg
     5281 ggaaacgtcg cgggcttgcc agcgttcgcg agcgcgctgc gcgacccgta gcaggccact
     5341 gccagccaac accagtagcg gtgctgagag caacaagccg aagaaccctg ggcgatcgga
     5401 gatgaacggc ccgcgcgtgc tcgctaggcc cagctgacgt gggggctcca cgagcttcgc
     5461 caatcggtcc tcttttccgg aaccggccac gggcgccggt gcattgggat cggcggcgac
     5521 ttcgatcgtt cccaggggtt gccgagccac ctcgtagcgg cgttgctcgg catcgtaaaa
     5581 gggcagcgtg agctcgccca aatcgatggt gccggccttc tcggcgcgca ccacgtaagt
     5641 gaaggttcgc gaaccagtca gcttgccgcc cgtcgtatcg agtttctcga ccacggtggg
     5701 ctccatccag tcgacgcccg tttgctgtgg cagcgcgagc ttcgagggaa tattaccagt
     5761 gccctcgagc ttagcgatga cagcgacggc ctcgccttgg cgcaccttcc tgggttcgac
     5821 gctcgctttc agactgtact ggccgacgtc gcccagtcgg taacctgcgg gccgccccgc
     5881 gagcggtggt tcaacgacct tgatgtcgat gggcaggctc tctcgtatga gcccgcccga
     5941 gccgcgcggc gcgcgataac ctcgtccgcc aaagccgaag cgcatggctc cgatcctcaa
     6001 ctcgccggtt tgaatcggaa acagcagcac gtcacgaatg cgcgccgcga tgtagctgcg
     6061 ctccccgata cggaccggta cggccgtgcg cccgtcgctc tcgaccgaat agctcaagaa
     6121 accttcgtga ctgggctcgt tggaggttgc ctcttcgtac gggcccgccc cgccgtacga
     6181 gtagatcttg agatgtaccg cttcccccag cacgacgctg cgtggggtcg ccttggcgat
     6241 caagaatgcg gtctgatccg gcgcgtgatc gagctggtat tcgggcggtg ccgccggttc
     6301 gggaatatct ggtgcatcca gatcgtcgaa cggaaaaccc ggaatacctt ggccgcgaaa
     6361 tgcactgaag ggatcgaacg gatcgagggg gaacgggaag cccgaagggc ggcgtccacc
     6421 tggtgggcta cggcgcgccg tgccggctgg cgtcacctcc acggtgattg cttgtccgac
     6481 gctgtgtttc ccgttcaggt cgacgctagg agggccaact cgaaaattac ccgcttggtt
     6541 cgcgaccaaa gaccaactca aggtgaggcc attgatgcgt tgcatccggc cattcgtgat
     6601 cgagacttgg gtttgcgtgc tggtgtgagg cccgctcact tgcaagccgc tcggcacggg
     6661 cagcttggcg ttggcgggtt cgacgtcttc atccccgcgg atgacgagct ctatgctgaa
     6721 tacctcgcca acttcgacgc tgcgtgagct cgcctgcgtg cgcagcacca actctgcgtg
     6781 ggccgcgttg acggagaaga tggttccaag gcctgcgctg ataccgagcg caagcgagta
     6841 cgggcgcgct ctagacatca accgaacgtg cggtccggca agtcggatca tttgtcctcc
     6901 atgccgtagg agcgagcttt gagggcgcgg ttcttcgcgt cctgctgctg cacggtggga
     6961 gcttgctcga gctgatcgag gatggcctcg tcctggctga cgttggagtc cggcggctgt
     7021 gcctggtttt cgtcttgccc ctgcgaagga ggctcctgct tgtcctgctg cgcctgcttg
     7081 tcgtcgttct ttgcgtcctt ttgtgcctgg tccttcttct cgtcgtcctt ctggtcttga
     7141 tctttcttgt cctggtcctt cttctcgtcg tcgttcttct gatccttctc cttgtctttt
     7201 tccttttctt gtttcttctt ttcttcctct tcgatgcgtc gcaacgcgat agatcgattg
     7261 taagcggcgt tgagtccgac gctctcgttg gccgttggcg cgctctgtga attcgcgctc
     7321 ttcctcggcg cgggcgcggt ttcgatgccg ggcgcgatct tcagcgcggc gtcgtagctc
     7381 ttcacggccg ctcgatagtc gccgcgcaag aactctagat tgcctgccaa gtaagaagcc
     7441 tccgcgcgga agtcaggcgc tgcgtcggtg ttgcccgcaa cgacgcgtac gacccgcagc
     7501 gcacactcca cctctcccga gcgttgctcg gccaaggctt tctgttcggg cgtgggttcg
     7561 ccatccggcg tggtcacttc ttccccgaag cgctgcccgt attgctcgcc gacccgaaac
     7621 agcgcgagcc ccagatcgaa actggcattt tcgcgctgcc gtatccgctc gggggtgccg
     7681 agctcaccct tctcgcattt gccggtcgag aggtattgtt cgagcagcgc cgctgcggcc
     7741 gcgccgtcgt tggccttcaa cttctcgatc gcggtttcga cgtcgggtgc agcgcgctcg
     7801 aagagggtct tctccgcttg cgcgcagctg ctcgagagcc cggcgaccag cagcaggccg
     7861 ggcagcgtgg tgatcccggc caccttcgct ctacgacggc gccgtttgtg cggctcgggc
     7921 ggcggcagta ccgccgggcg cgcgcgcggt gcggtctcgt tcacgaacac ctcgagtaag
     7981 agcaacagcg ccgccaaccc gagcggataa aagaagacgt cggcgtagac ggtctccacc
     8041 cgttcggaca actcctctgt catcagcttg cgaaggcgtg cggcgatcac ctcgatgccg
     8101 gttttgccgc ccgcgctgcg cacgatctgc ccgcccccca cgctggcgat cttcgtgagc
     8161 tgctcttctc ccgccgccga gagcgaagta gtgagcggct taccttcggc gtcttgtcgg
     8221 tagcccttca cttggcccga ctcattcacc tctgggattg gctccggcgt acgtccaccg
     8281 acctgcacca cgtcgatcga gatctcctcg ccggccgcgt tctgcgccac cgccactgga
     8341 tcgccttcca ggtcttcacc atcggtgacg agcagcatca ccttcttgtg ctgcttggag
     8401 agcgggtcgc gcgcgaatag atcgcgcccc gcctccaggg cccgcgcgat ggcagtgccg
     8461 cccaccggca tgtcattggg ggccagttga cggaagaact gggagatcgc gccgccgtca
     8521 ctggttagcg ggaacgccat cggttgcccg gcaaaagcta cggctccgaa gcgagcgccg
     8581 ggcagatcgg cgatcaagcg ggagacctcg ctcttggccc gcaaagtgcg tgaaggcgcg
     8641 atgtcccggg catacatgct cttcgagaag tcgagcacta ccaccacatc caagttggtg
     8701 gcgggcacca tgcgcgtgcc ccgcccgtac tgcggctggg ctagcgccaa gaaagcgagc
     8761 gccaccgcgg tcacgagcag cccacccttc agggcgcgcc gcccgccggg tcgccccgtt
     8821 acgaggctca acaccaacgc ttcatcaccg aaacgccgag tggcgcgcat cagcaagaag
     8881 ccacccgcga tcaagagcac cgccacggcc agcgccagcg cactaccgaa caaccaatat
     8941 ggggctgcga acctcacggg aacctccgca ataccagcga cctgagcagc gcttccaggc
     9001 tcaggagcaa caccccgggc aacaagaagt agccgaacaa gtcctcgtaa tgggcgatcg
     9061 atgcctcgag cttggtcttc tccaagcgat tcagcacatc atggaagctt gcctgtaagc
     9121 tcttcacgtc cgtggctacg taggccgccc ccgaggtctt ctgggcgagc gtctgtaaca
     9181 acttcggatt cactggaaac gagcgttgga cgtaacgcgg ttgcccgaac aaatcgaagc
     9241 cgtcctgcac ctcggccgtg tcaccttgcc cgatttgcac cgtgaacagc ttggcgccta
     9301 cggtgttggc caagtgagcg gcgtattcag gggctagctt tccgcccttg ttgtcgccgt
     9361 cggtgagcag gatcacggct ttgctcttgg ccgagctctt acgcaggcga gccacggcca
     9421 caccgactgc gtcaccgatc gccgtgccgt tcgcgtccac cagaccgagc tccatcttgg
     9481 ataccaacac gtcgagcaag tgatagtcga gcgtcggcgg cgagagcacg taagcgctcg
     9541 agccaaacac gaccacaccc agacgatcgc tctttcgacg cgcgatgaag tcccgcatca
     9601 cggccttggc gccgtcgagc cgcgtggggc gaataccgcg cggtttttcg gggatgtagc
     9661 gcgccagatc gggcggtacg ttgctgagcg cggcctgcat ggaaccagac atgtcgagcg
     9721 ccaacatgat gtcgatgccc tcttcgtccg tggtttgggg gcgcagggta tccactggtc
     9781 gtgccatcgc gagcacgaac agaatcagcg ccaccgagcg cagtactccg ggcaaatcgc
     9841 gcagccacac gcggatgccg gatgggccga ccgcgagcgc cgagagcgtg cccagcttga
     9901 gccggggcat gcgccggtct tcgccccaaa tgccacgcca gaagaccagc gggaccagca
     9961 cgagcccgag caaaaaccag cgttgctcgt agttggcgtc gaagaagtcc gtgccgcgcg
    10021 tcagcgccgg gtaggcgaac ccgaacagca gcgcgagcag cgtgaagccg aacaccgtga
    10081 gcgcacgcat caggcggggg cggtgcttca cggcgcctcc ttcgggggag ccggatccgg
    10141 cttttcagta cctgtgattc cgttgtccgt cgccgtgccg gaggcgccgt cggccgccgt
    10201 tccgtcggcg ctgcccggtt gcggcgcccc gagcggcagt gcgggacggg tcagcgcgac
    10261 caaggcctcc gcccgcgtga gcgcgctcgc gcactgcgcc tcggtggggt cgttgcgcgc
    10321 gaacttcacc aaatccgcgt cctgcatgaa ctcctgaacc ttgacccaca catcgaacgg
    10381 cacggatacc ctgcgcagcg ccgcgagcgc ctcacgcgtc gtgctctcca gcccttcgta
    10441 cccgtagcga tcgcccaagt actgccggat gatgtccgag actcgctcgt agaattctcc
    10501 gtaacgctgc tcacggagca gccccgagtg acccacgtcg aacaagccct ccagcgcaac
    10561 ttcccacggc ggtcggggcg ggggaggcgg agccaccttc ttggggcgtc gcatccaacg
    10621 gctgatgatc aaagccagca gggccccgag cgccagcgcg atcaggccag cgtaagtgac
    10681 gtgtttggcg agcgtccact cctcgaattg ccgccgtgga gccgggttcg gcttcggttt
    10741 ggggttcgga tcattggcga tgggatcatc gacgatgagc gcgtgcgagc tcgtgcacag
    10801 cgtcatcatc tcgccgctcg ctcgtgagat cgcgatcggc agcgcgggca acgtcagctc
    10861 ctgtcggccc ggcttatcgg gtagcggcac cagctggagc tgcacggtcg tgcgggcgcg
    10921 tcctccctct tcactgcggg tcaacgtggg cttggcgggg ccccgggggt cggggatgaa
    10981 gaagtgtgcg ccctccagcg ctttgtagtc atctccgccc gcgtccagct tgaagccacc
    11041 cggcagtacc gtctcacctg gcccgtgctc gacgacgagc tcgagtgtga gcgcgtgtcc
    11101 ggtcaccccg tgcgccggaa atttctctgt caactttggt cgtgcttttc cgtcggggag
    11161 gcgctctacg cacgtccccg tggattgggt atgcgtgggg ctcgccgccg ggaccggcgc
    11221 tgaaaccgcg ctactcccgg cggcggcgct ggcggctcct ggaggcgcct gcgcgcccgt
    11281 tgtcacagcg ccttgcgcct gcgccgcgtt ggaactcgcg acgtggacca gtagagcaag
    11341 gccaactctc cagcgcgcca tcagcggctt ctcctcgcgc gccgcgcgaa caagtcgcgc
    11401 aacggtcgga tatacggctg gtccgtgcgc accaccacgt gatccaagcc gagcttcctg
    11461 aacaacttga cctgtccttc tcgttgcgcg cgcatctgct tggcgtaatg agcccgcacg
    11521 cgcttgctcg aggtatcgac caacacgctc tcacccgtct ccaagtcctc gaacgctgcg
    11581 agccccacgt cgggaagctc ctcgtctcgt gggtccacca gcatcacggg aatcacgtcg
    11641 tgccgcgcgg cggctaggga caacgctcgc tcgtgaccct gggcgaaaaa gtcactcagc
    11701 acgaaagcaa cgctgcggcg ccgcgcaacg cgatacaacg tctccagcgc caccttcaag
    11761 tcggtgccgg ggcgctcggg ggtcgccccc aaaatctcac gcaccacacg catcacgtgc
    11821 ttttgcccct tcatcggggg cacgatcttt tcgatctgat ccgtggataa aatcagcccc
    11881 acgcggtcgt tgtgcttgat cgcgctgaac gcgcacagtg ccgccacctc cgccgcgagg
    11941 cgagacttgt tcgcggtgcg cgtgccgaac tgctgactct ggctcaaatc gacgaccagc
    12001 atcacggtca tctcgcgctc ttcgacgaac accttcacgt aggtgttgtt catgcgcgcg
    12061 ctgacgttcc agtcgatgct gcgcacgtca tcgccgggct ggtactcgcg gacttcccgg
    12121 aacgcgagcc cttggccctt gaacacggac gtgtaattgc cggacagctg ctcggtcgcg
    12181 agccgattcg tcgtgatctc gatcttgcgc agcgccttga gcaactcttt cgggatcacc
    12241 gttcctccaa atcaagcatc gtagccaggc cccgacgcac cgcgcttcag ggcacctcca
    12301 cgacctcgaa aacgcggcgc acgatgtcct cggccgtcac ctcttccgcc tccgcttcgt
    12361 aagacaacac catgcggtgg cgcagcacgt cgggccccac cgctttcacg tcttccggcg
    12421 tgacgtagcc acggtgtcgc agaaatgcgt gcgcgcgggc ggccaggttc aaggcgatgc
    12481 tcgcgcgcgg gctcgcgccg tactcgagca gcggcgccaa atccttcatg cctttggccg
    12541 aaggctcgcg cgtcgcgaac accacgtcca cgatgtagtc cttcacgcga tcatccatgt
    12601 acacgtcgcg caccaccttg cgcgcctcga gcaagcgctc gggtgtcgtg cgcgggctaa
    12661 tcgacgcggg cctcgtctgc gtcatgcggt ccatgaccgc gcgctcctct tcgcgagtgg
    12721 gatagctcac cttgatcatc aacaagaagc ggtccacctg cgcctcgggc agcgggtacg
    12781 taccttcttg ctcgatcggg ttctgcgtcg ccatcaccac gaacgggctc ggcagtgcat
    12841 aagtgcgatc cccgatcgta acctggtgct cctgcattgc ctcgagcagc gcgctctgaa
    12901 ccttggccgg agcgcggttg atttcatccg ccaacaccag gttcgcgaag acgggcccca
    12961 gcttgctcgt gaactcaccc gtcttttgat cgtagatcac cgtgccgatc aagtcggccg
    13021 gcaacagatc cggcgtgaac tggatgcgcg aaaacttcgc ggccacggcc tcgcagatgg
    13081 tgcgcaccgt caacgtcttc gccaacccgg gcacgccctc gagcaacacg tgcccgcctg
    13141 cgagcagccc gatcagcacg cgctcgatca tgtacgtctg acccacgatc accttgccga
    13201 tctcggcggt caggtcatcg atgaacgcgc tctctctcgc gacgatctcg tttagtgctc
    13261 ggacgtcttg catgctttcc accgtgttga ttcgtagact tgcggttgat tcgtagcttc
    13321 gcgtcgtcag gggctgcgtc gggtcccaag cagagggagc aggctcccgc ttgcgagccg
    13381 cgtatagcgc gaccggccgg ccacagacaa tctaaccgcg ccgcatattc ccgtttgccg
    13441 tggagaggct gagtcggccc acaaaaaggc agcaaaagcg ctggccaaac ggccgtcagc
    13501 catctcaatc ggagccgcat cccgcgcttt cccgctcgtt cgaagcggtt tggcggcttg
    13561 ggattggctc ggcctcggaa caaagagcgc gccttcgcgg cgtgaacgcc cgccagcgca
    13621 gtccaattcc cgaggagcac ggcgcatcac cgcgcaggcc accccaaata acgcgtccgc
    13681 gggaggcaat ctgcccgacc gcggggaagt tcgcggctcg cgggtgcgta cacccgaacg
    13741 agtcagggag ggtttgcggg cgtgagcacg aacaatccga gcaagaacgg gcccagctcg
    13801 aatgagacaa gccaagccag cgcaggcaag acgggcaaac gtggcaggcg aacgctacgt
    13861 ggtctccgcg gcgcgctctt cagctccgtg ctgttcgtcg gactggcgcg ttcgagccac
    13921 gcccaaacct acactccaac ggacaccgcg cgtgaggttc agctccggag cgcgttgctg
    13981 agaaactcgt tcgagagcgt gagcgttccg ctccgccgtt acgcccccgc cttgctcggt
    14041 tcagcgtttc tcgtgggtgc agcgggcttc gcgttcgacc ctccgcccac cgggaagttc
    14101 gaagagaagc gctggcatct gacggcgggt ctcggtacct tcgccgcttt gtcattcggt
    14161 acctacgctc tccccacccg atatcagggc accacgttcg ctctcttggc cggagcgggg
    14221 ctggccggcg cgggcgcggt tgggtgggct tggagtgaac aggatacgcg gtcggcgcgc
    14281 gtagtatttg gcagcatgct gggcgccggg atcctgaccc tcggcctcga agccctggat
    14341 accgcgctgc tcacgccgct ggatcaatcg gagatcgagg gtcacttacg ccggctcggc
    14401 cggcgagggg cagcgccgag ccgcgcggag cttcgacgca tggagtggga actctcgctc
    14461 acgcgccggc gcatccccgc atggctggtg ccactcaccg tgaccctgag cggagtggtc
    14521 gcggcgtcac ctctgcttgc ctttccagac gcgagcaaaa gcgatcgagt gctgtcggcg
    14581 ctggtgtcca tccaactttt gcaaatcggt aacgctggcc tcatctcggc gcggagccgt
    14641 gacactggtt atcccgctta cgttcgcgcg ctccgaaacg tgaggttggc cccgctcgca
    14701 gggggcggcg ccaacgtgag cgtgagttcg acctggtgac gccacccgcc aacacccgtc
    14761 acgagtagga cttagtcgat ttcgcgatac tcgaaccagt cgaagtcggc gtggtttcta
    14821 gaaccggaga gatcctggca agccatgccg acgaagtttc cggtaaagcc gcaaggtacc
    14881 gcatgctcgt ccgacaatat cgaagcgtcg tattcccagc cggcggcggt ccatagcttg
    14941 ccatcacgcg agtagtagaa ggtgatgcgg tcgtcgcgaa tca
//
LOCUS       DASTKU010000766         6276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_372937, whole genome shotgun sequence.
ACCESSION   DASTKU010000766 DASTKU010000000
VERSION     DASTKU010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372937"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3013
                     /locus_tag="VFQ61_26715"
     CDS             <1..3013
                     /locus_tag="VFQ61_26715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9958128.1"
                     /translation="GVRLGTPRYMAPEQIRDPRAVDGRADVFALGSVFFEVLTRKPAF
                     PADDALGAIAQILLDEAPDPRRYTPTLPEEVAMLARSLLTRDRAKRPYANRELAEHFQ
                     KMAERYRAWNPGELNASHTLGSAPAGLAAPTAAGAPASRYPALVSGTSLPGATSLDPP
                     GPFPMRSQRGSAQLEGPRFVGRVSELQALSAALNTGSRVVLLWGAAGIGKTRLAHAVT
                     VQALGVPAAAEHAGLDGEESPGAPGFRFIDLSQARDKNDLVRLVLAEIGATNIGAEDA
                     ERALLRALARGQAGSWLVLDRAEHISAPVQEFVQAALGSSAVRFLVTSRERLRLTECT
                     AIELGPLAVRAKSELRTLLGFDKAATPSTRRDGGGAGPNGEVSEGALLLLSRIAELEP
                     RFVATQRDLYLAELCAAALEGVPLAIELAALRVSILGLEGVVGRLSEQLSLLGGPEQQ
                     TAQRRQRTMQDALSASWQLLTSDERELLMQCSVFRGGFTVAAAEAVLAPGPGSSILDV
                     LQALRDKSLLFTPPTRTAADGEPRLFTFAVVREYASAKLAESGRLAELCARHARYYIE
                     RFAKFANLDRSEWTRSRLRALEAESENLLAAVEHALSEPARNIRLALEVLLVLEPLIA
                     LRGPLPAFSTLLEAAIEAGERCAAELPAALLPHVRRIRARLLATAGQFGPAIQQLELA
                     LSESNLDPVARGLCHLELGVVHHLARELPKAKQHYDAALTDLRESAEPKARHGVPVGP
                     KLLRAQARCLGNLGAALHDLGELAAAAEHYAEAIERLEELGEKRERANFLGNLGILDQ
                     ELGNIERARRHLNQAIALLTEQGDARLCGIALGNLGSLEAELGDWSAAVSCHERALEM
                     LRPLDDRCSEALCLGRLGAAYATLGRHDLAEDYANRAFDRLNLGSSASQSDVQGAGTM
                     NSADLAHGETLHLLGAFLDLEEARSALRQEPGEARAHLRRAEARVHRAESMLVGGIPL
                     RTLSDDTRVSLRVLKPLLACLHEELSRGSAL"
     gene            complement(3637..4470)
                     /locus_tag="VFQ61_26720"
     CDS             complement(3637..4470)
                     /locus_tag="VFQ61_26720"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9958129.1"
                     /translation="MRHYWLEFGGRILELRPGTLLIGRSGTADLVLDDALVSRRHARI
                     HADAWGASVEDCGSINGVFLNSRRITQREPLQDGDKIQIGTQVLVLRSALARPKEQQR
                     DRRTVDTLLGERSVKPSASVLNIASESEATFSAHTLDLLGGVAEKILALGRAEEAEKV
                     LTQTLQNILSGVLAGKTEQCPPPICEKAVYHAVKLAEGTGRGRWVDYAVELYTALGRP
                     LPAPVVDQLYGVLRRVDVINLPALRGYVAVLRAMQRDFGPSERFVLQRLEGLERLFAL
                     K"
     gene            4649..5503
                     /locus_tag="VFQ61_26725"
     CDS             4649..5503
                     /locus_tag="VFQ61_26725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958130.1"
                     /translation="MRKLSSSSQIFAPAFLTYLITAGVVRAEPIAPVLSPSSGLAPTS
                     ESQTRPGASDMPEPPSSPTNPPPANPAPPPTLPAAQSNVETGTTPSAPDSAPQFGAPT
                     PTMPKTNEGSRANGNSSTSAGVALRMQVNYAGSWLEARSFDENWAGSSGFRRLCFAPC
                     RDVRVSAEQEIRVLAPGMTPSPVFRLQPGSGAALLTVRGGSQWLRTLGRVGLVVGLPV
                     AGLGMAGLGYGAFEDQAGLRNAGAVTLGVGGVLILAALPLLVLGSTSVLNDRGERVAT
                     AFDTAKRW"
     gene            complement(5605..>6276)
                     /gene="serC"
                     /locus_tag="VFQ61_26730"
     CDS             complement(5605..>6276)
                     /gene="serC"
                     /locus_tag="VFQ61_26730"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HET9958131.1"
                     /translation="LELDANAAYVHITSNNTIFGSQWHAFPETGNVPLIADMSSDIMW
                     RPIDVSKFGLIYAGAQKNLGPSGLCVVIVRKDLVASARKDIPVIFQYGTHSENNSLYN
                     TPPTFSVYVLRNVLDEYKRLGGLAAIEKVNREKGRVLYDAIEQRPDIFTAPVEKDSRS
                     FMNVVFTLKTPELEAEFLAEAKKRGMEGLKGHRSVGGMRASTYNAVSLEWVSALADFI
                     KNFKK"
BASE COUNT         1085 a         2063 c         2039 g         1089 t
ORIGIN      
        1 cggggttcgc ctagggaccc cacgctacat ggctcccgag caaatccgcg atccacgcgc
       61 ggtcgacggg cgcgcggacg tgttcgcgtt gggctcggtt ttcttcgagg ttctcacgcg
      121 taagccggca ttccctgcag atgacgcgct gggagcgatc gctcagatat tgctggacga
      181 agccccggat ccgcggcgct acacaccgac tctcccggag gaggtggcca tgctggcccg
      241 ctcactgctc acccgcgatc gcgccaagcg accatacgca aatcgcgagc tggcggagca
      301 ctttcaaaag atggcagagc gctaccgcgc gtggaatccc ggcgagctga atgcgagtca
      361 tacgctcggc tcagcgccag ccggcctcgc cgcgccgacg gcggccggcg ctcccgcctc
      421 acgctacccc gcactcgtca gcggaaccag cctacccggc gcaacctcac tggatccacc
      481 cgggcctttc ccgatgcgat ctcagcgcgg cagcgctcag ctcgaagggc ctagattcgt
      541 cggtcgtgtt tcggagctgc aggcactctc ggcagctttg aatacgggtt cgcgcgtggt
      601 cttgctgtgg ggagcggccg ggatcggcaa gacgcggttg gcccacgcgg tgacggttca
      661 agccctcggc gtgcccgccg cagccgagca tgccgggctc gatggagaag agtcgcccgg
      721 cgctccgggt ttccggttca tcgatctctc gcaggcccgc gataagaacg atctcgtgcg
      781 cctggtactg gcagaaatcg gagccacgaa catcggcgcg gaagacgccg agcgggcact
      841 gctccgggcg ctggctcgtg gccaagccgg ctcgtggctc gtgctcgatc gcgcagagca
      901 catctcggct ccagttcagg aattcgttca agcggcgctc ggtagcagtg cagtgcgctt
      961 tctggttacg tcgcgcgagc gacttcgcct caccgaatgc accgcgatcg agctcgggcc
     1021 gctcgcggtt cgagcgaagt cggagctacg cactttgctc ggcttcgaca aggccgccac
     1081 cccttcgacg cgacgtgacg gcggaggggc ggggccgaac ggggaagtct ccgaaggcgc
     1141 gctactgctg ctgtcccgga tcgctgagct cgaaccacgc ttcgtggcga cgcaacgcga
     1201 tctctacctc gcagagctgt gcgcggcggc gctcgaaggc gtgccgttgg ccatcgaact
     1261 ggccgcgctg cgcgtcagca ttttgggttt agagggagtg gtggggcggc tcagtgagca
     1321 gctcagcctc ctgggagggc ccgagcagca gacggcccag cgccggcagc gaaccatgca
     1381 agatgcgctg tccgcgtcgt ggcagttgct cacgtcggac gagcgcgagc tcttgatgca
     1441 atgctcggtg tttcggggcg gatttacggt ggcggcggcc gaggcggtct tggcgccagg
     1501 ccccggcagt tccatcttgg atgtgctgca agcactgcga gacaagtcgc tgctattcac
     1561 tccccccacg cgaactgccg ccgacggcga gccgaggcta ttcaccttcg ccgtcgtccg
     1621 cgagtacgcg agcgcgaagc tcgccgagtc cggtcggctc gcggaactct gcgcgcgcca
     1681 tgctcgctac tatatcgagc gcttcgccaa gttcgcgaac ctggaccgct cggagtggac
     1741 tcgctcgcgc ttacgtgccc tcgaggccga gagcgaaaat ctgctggccg ctgtggaaca
     1801 cgcactgtcc gaaccagcca ggaacatacg gctcgcattg gaggtgctgc ttgtgctcga
     1861 acctttgatc gcgctccgcg gcccgctgcc cgcgttctcg acgctcttgg aggccgcaat
     1921 cgaggccggt gagcgctgtg cagctgagct ccccgcagca ctgttacctc acgttcgacg
     1981 tatccgagcg cgtttgttgg ccaccgccgg gcaattcggt cccgccattc aacagctcga
     2041 gctcgcgctg agtgaatcca atctagaccc ggtggcacgc gggctctgcc acttggagtt
     2101 gggtgtggtt catcatctgg cgcgcgaact cccgaaggcc aaacagcact acgacgcggc
     2161 gctgaccgac ctgcgggagt cagccgaacc caaagctcgg cacggcgtcc ccgtcggccc
     2221 caagctgctc cgggcccagg cacgctgtct ggggaatttg ggggcggcgt tgcacgatct
     2281 cggcgagctc gcggcggccg cggagcacta cgccgaagcc atcgagcgac tcgaagaact
     2341 gggcgagaag cgcgaacgcg ccaacttttt gggtaaccta ggcatactcg atcaagagct
     2401 cgggaacatc gagcgcgcgc ggcgacattt gaaccaggcc atcgctctcc tcaccgaaca
     2461 gggtgatgcg cggctctgcg ggatagcgct tggaaattta ggcagtctcg aggcggagct
     2521 cggggactgg agcgcggccg taagttgtca cgaacgagcg ctcgaaatgc tccgtccgct
     2581 cgacgaccgt tgttcggagg cgctctgcct tggccgcttg ggcgcggctt acgcgacgct
     2641 cggtcggcac gatctggccg aggactacgc gaaccgcgcg ttcgatcggt tgaatctagg
     2701 cagttcggct tcccagtccg acgtgcaagg agcgggcaca atgaacagcg ccgatttggc
     2761 gcacggcgag actttgcacc tgctcggcgc gttcctggat ctcgaggaag cacgctccgc
     2821 gttgcgccaa gaacctggcg aagcgcgggc ccatctccga cgcgccgagg cgcgcgttca
     2881 ccgtgccgaa agcatgctgg tcggggggat tccgctgcgg accttgtcgg acgatacgcg
     2941 cgtgagcttg cgcgtgttga agccgctcct cgcatgctta catgaagaac tgagtcgtgg
     3001 ctccgcgctc tgactgaatg gctcgctctg aatggctcgc tctgggtgcc gcgtcagcaa
     3061 agcgcgccgg ctgcgcaatg cgggtgccga gcggcgcgcc ggggactaga ctcccgcgca
     3121 tgcacgggcc tccctctccg cccaagaagc ccagcgcgct gggtgacctg ttggaagaca
     3181 cccggcgcgt cacgctgcag cgctcgcgtg ccggggtgga tcgggagctg tttcgcaaag
     3241 cggtgggccc gcgcattgcg gatcgcaccc aaatcggacc tttgcagcgc ggcgaactca
     3301 cgatcttcgt ggcctcggcg gcgtggtctc aagagctgag cctgctcacc acggaaatca
     3361 tcgaacgcct cggcgcgcat ggggtacggg tcgagaagct gaaattccga gtcaaagcga
     3421 gcgcaccgaa atcgaccgcg cgcaaagcac cgcggccgcc cccggtgcca ctgccgccgg
     3481 aattgacaca gcgactcggg cgagtgggcg acgacgagct tcgagacgct attggcgcgg
     3541 cggcggcgtt agcctttgcg cgactggaac acgaagcgag agccgctcga aatccgatgc
     3601 gcgcgcgaga gccggcggct tcgacgagcc gtccgcctac ttcagcgcaa aaaggcgctc
     3661 caggccctcg aggcgctgga gaacgaagcg ctccgacgga ccaaaatcac gctgcatcgc
     3721 ccgcagcacc gctacgtaac cgcgcagggc ggggagattg attacgtcca ctcggcgcaa
     3781 cacgccatag agctgatcga ccaccggtgc gggtaagggg cgaccgagcg cggtgtacaa
     3841 ctccacagcg taatcgaccc atcggccacg gccggttccc tccgccaact tgaccgcgtg
     3901 gtaaacggcc ttctcacaga taggtggtgg gcattgctcc gtctttcccg caagcacacc
     3961 actcaggatg ttttgcaggg tctgggtcag cactttctcg gcctcctcgg cgcgccccag
     4021 agccaaaatc ttttcggcga cgccgccgag caagtcgagg gtgtgcgcgc tgaaggtcgc
     4081 ttcggactcc gacgcaatat ttagaacgga cgccgagggc tttacggatc gttcgcccag
     4141 aagcgtgtcg accgtcctgc gatctcgctg ctgttctttg ggccgcgcca gcgcggagcg
     4201 gagcaccaag acttgggtgc cgatctgaat cttgtccccg tcctgcaggg gctcgcgctg
     4261 cgtgatgcgg cgcgaattca ggaacacgcc attgatgctg ccgcaatctt cgacgctcgc
     4321 tccccaggcg tccgcgtgga tgcgtgcatg ccgtcgcgag accagcgcgt catccaaaac
     4381 caggtcagcg gtaccactgc gcccaatcaa cagcgtgccg ggacgcaact ccaagatgcg
     4441 cccgccgaat tcgagccagt agtggcgcac gccctgccct cccccaccag cgtctggtgc
     4501 aggcggcgct gatctcgacg tgcgttcttc agccacgccg cacaaggcta atcgtcggcg
     4561 cgcaaacgag caatcaccga gttggctgaa ttgtcgtcta ggatggccgc gccttcccgg
     4621 aactcgcggt tggtaagcga aactacgtat gcgtaagctg tcatcgtcgt cccaaatttt
     4681 tgctccggcg tttctgacgt acctcatcac tgcgggtgtg gtccgcgccg agccgatcgc
     4741 accggtgttg agcccctcct cgggcctcgc tcccacgtcc gagtcacaga ctcgaccagg
     4801 agcgtcggac atgccggagc cgccctcctc cccaaccaac ccgcctccag ccaatcccgc
     4861 gccgcccccg accctgccag cagctcaatc caacgtggag acgggcacga cgccctccgc
     4921 cccagattct gcgccgcagt ttggggcgcc gacaccgacg atgccgaaaa ctaatgaggg
     4981 aagccgcgcc aacggcaact cttcgacgtc ggccggcgtg gcacttagga tgcaggtcaa
     5041 ttatgccggg agctggctgg aagcccgctc tttcgacgag aactgggccg gcagctcggg
     5101 ctttcgccga ctgtgctttg caccgtgccg ggacgtgcgg gtctccgccg agcaggagat
     5161 tcgggtccta gcacccggca tgaccccctc gccggtcttc cgccttcagc caggttcggg
     5221 tgcggcactg ctcacggtgc gcggcgggtc ccagtggctc cgcacgctgg ggcgtgtagg
     5281 tctcgttgtg ggactcccgg tcgcaggact cggaatggcc ggactcggct acggtgcctt
     5341 tgaagatcaa gcgggattgc gaaacgcagg cgcggtcaca ctcggcgtag gcggcgttct
     5401 gatcctggcg gcgcttcccc tcttggtgct ggggagcacg agcgtgctga acgaccgcgg
     5461 ggagcgcgtc gcgaccgcat tcgacacagc aaagcgctgg tgaaccgaga ctcgcgctca
     5521 ggtcgagcgc ccgagaaaga acccctgaaa tgcaaacagg ccgcccgagc tgctcaggcg
     5581 gcctgcgaca gcgctaggga gcgctcactt cttgaagttc ttgatgaaat ccgcgagcgc
     5641 cgagacccac tcgagggaaa cggcgttgta cgtggaggca cgcatgccgc ccacggaacg
     5701 gtgccccttg aggccttcca tgccgcgctt cttggcttcg gccaggaact ccgcctcgag
     5761 ctccggggtc ttcagcgtga agaccacgtt catgaaagag cgcgaatcct tctccacggg
     5821 ggccgtgaag atgtccggac gctgctcgat ggcgtcgtaa agcacgcggc ccttctcccg
     5881 gttcaccttc tcgatggcag ccagaccacc caggcgcttg tactcgtcga gcacgttgcg
     5941 caggacgtac accgagaacg tgggaggagt gttgtacagc gagttgttct cggaatgcgt
     6001 gccgtactgg aagatcaccg ggatgtcttt gcgggcgctc gcaaccaagt ccttgcgcac
     6061 gatcacgacg cacagaccgc tcggcccgag gttcttctgc gcaccggcgt agatgagccc
     6121 gaacttcgag acgtcgatcg ggcgccacat gatgtcgctc gacatgtccg cgattagcgg
     6181 aacgttcccc gtttccggga atgcgtgcca ctgcgagccg aagatggtgt tgttgctggt
     6241 gatgtgaacg taggccgcgt tcgcgtcgag ctcgag
//
LOCUS       DASTKU010000767         7042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_373188, whole genome shotgun sequence.
ACCESSION   DASTKU010000767 DASTKU010000000
VERSION     DASTKU010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373188"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1064)
                     /locus_tag="VFQ61_26735"
     CDS             complement(198..1064)
                     /locus_tag="VFQ61_26735"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9958132.1"
                     /translation="MTDLVSAALCHNLLLVFDDPTYRPPALPGVALELMAISGQPDPD
                     LGEVVALLERDAMLAGTVLRLVSSPFYAGRARIRSLREAVVRIGVRGVRDAVFEAALR
                     QGVFAMREYADTAQTVARHSTVTAYVAKLIARRAHLDADTAFLGGLLHDVGYAGLLLA
                     VAHVEGDDAPGLAPMWGDVCSLHPRASRMLAERWGLPWEIAEIAGSHHDSSAASTSRV
                     LAAVRLADHLSESLGAPVLGPLVGGRPLPSVVGNVSVDLPSLMRELCIDEVDLAQILE
                     DAERVIGEIVAL"
     gene            1401..1985
                     /locus_tag="VFQ61_26740"
     CDS             1401..1985
                     /locus_tag="VFQ61_26740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958133.1"
                     /translation="MPTPPNPPTGPHVDLDDDPLLPWLVATRSGDRSAEHELLLRLAP
                     ITLGLARRLLPDTRTRPDNPKDQSALAFQALLLVLRALPSLRGEERTSRAVAKLTLEA
                     ARRFPGTSTIAETEALSAARELEQATARGRDDRLVHELADRAFRDDSSILVSQVIVKP
                     GEATRRSRLRLALAALGLLTAAWLGYLLGTRRNR"
     gene            complement(2003..2686)
                     /locus_tag="VFQ61_26745"
     CDS             complement(2003..2686)
                     /locus_tag="VFQ61_26745"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HET9958134.1"
                     /translation="MIQLGLAAALWFALHAGIAGSGLRRVLIARIGEKAYRGVFSLLS
                     VVTLVFLVHAYGAAPCVPLWSVPDWFRWLPISSMPFALFLLVGAFSVPNPTSVGGEAL
                     LARKVEARGVLRITRHPFLWAAALWSTVHLLVSGTLTGLLFFGSIGLTALQGTRDIDE
                     KRRRAQPLEFEALREKTSNLPFAALIRGRNRLVWRELALPFVLAMALLLGILSQHQRW
                     FHVSPWPFA"
     gene            complement(2695..3171)
                     /locus_tag="VFQ61_26750"
     CDS             complement(2695..3171)
                     /locus_tag="VFQ61_26750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET9958135.1"
                     /translation="MTIYDLQTKTLEGTPVSLERYRNQVSLIVNLASACGYTPQYAGL
                     ERLQRRFQERGFTVLGFPCNDFGAQESGSPEEIRTFCESRYEVTFPLFEKVHAKPGAG
                     QSDLYGRLERAAGVAPAWNFGKYLVSRSGDVLEYFPSSVEPEDPKLIAAIERALSV"
     gene            3559..6375
                     /locus_tag="VFQ61_26755"
     CDS             3559..6375
                     /locus_tag="VFQ61_26755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9958136.1"
                     /translation="MSDTAWRILIVDEQPEQRAHWRRLLLEGSEREYQFTEVESAQAA
                     CKLFTAVNPQFTCIIAPDDTASSDGPLLLNRELTRAPVVVVTTSRSHRHAKDLLARGA
                     ADYVGAHWLGPEALTRAVERAVETWDLEAALTHARQRECRSSSGPGLVDACNERGPAS
                     LSRALEEREKLLGLALEATETGLWTWNLATGHVMWSPECYRIHGLTPDEFVGSAAGFF
                     ELVHPEDRARVETTVHSAIRDRHLYECVFRILRPDASVLWVENRGRASYDTEGQATRM
                     LGTIRDVTQRRRTEEAAGRAEALVQAVIDGTEALVFAKDLEGRYFLYNRQWLEFAGMT
                     AEEACGITDDRVFGAAAAQLRENDRRVIESAAPLRTEERATLRGRECVYLSHKFPLRD
                     PNGNTYGVCGISVDITELEKLQQSLSDSKRELQTLADNSPDLLTRFDKQLRHVFVNAA
                     AEKLTGLPSAAFLNKTNRELGMPPELCKLWEAGLQRVFQTAEPESLKFVFDSGNGLKH
                     FESRLVPELGLLGEVTHVLGLTRDVTDRHRYEEALLLADRRKDQFLATLAHELRNPLA
                     PLRSGLDILRAENRYPDSGQKTLQIMDRQLNHLVRLVDDLLDVSRIASGKVVLRPELV
                     SMQWVVNAALEATQPCLDAARHHVHLDLPGDAVWVRGDPTRLAQVATNLLTNAAKYTP
                     DGGRIRVSLSAGADTARLIVQDSGIGIPERMLAHVFELFAQVNREQKRKQDGLGIGLA
                     LVKSLVAMHGGSIAVHSAGEGLGSTFTLELPRALERQGPQEQPARLEPEATSFPPRRV
                     LVVDDNEDAAELLGSALELKGQDVRTAHDGRSALLTARQFRPDIIFLDIGLPDMDGCE
                     VAKQLRAQPEFREALLIALTGWGSDEDKRRTASAGFDLHLTKPVEAHSVARVLSHPTE
                     SGPSTSAPEVSREREQPPSYGQEA"
     gene            complement(6384..>7042)
                     /locus_tag="VFQ61_26760"
     CDS             complement(6384..>7042)
                     /locus_tag="VFQ61_26760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HET9958137.1"
                     /translation="AFQELKVPFDANDADTRLMTDWFARNSGLREAYQPGVDECRGIL
                     ERLGDHAPSHLDLAGAYLREEKLPEARLHAERALELGFAAPGLARNMLAVIAQRSGQI
                     ERMQEQFLLAARSDPQHYVLIRNVEAARSWFKRGGPALGLPLELDGRHDFQLFERTEQ
                     PTLPGPLPPDVCEWPAPLTPPRANVDSSVLELPESHQVLRIDTGQEALPFGNRRLPVV
                     "
BASE COUNT         1393 a         2292 c         2197 g         1160 t
ORIGIN      
        1 aaggtgagcg tgcaagggac gcccacgctg ttcttgaacg gcgcacgcat cgagaacccc
       61 accgacttcc aagccgtggc ccaggccatc gacgacgctc tggctggcaa gaccccgccc
      121 ggctaagctc ggtcgagact cgcgcgggtc gagttcactc ggcccgcgtt cgagcggcgt
      181 gagtcacgca gcgccggtta cagcgcgacg atctcgccaa tgacgcgctc tgcatcttcg
      241 aggatttgcg ccaaatctac ctcgtcgatg cagagctcgc gcatcaagga gggcaggtcg
      301 acggagacat taccgacgac gctcggtaac ggcctacctc ccaccaaggg tccgagtacg
      361 ggggcaccga ggctttcact cagatggtcg gcaaggcgaa cggccgcgag cacgcggctc
      421 gtggacgcag cactggagtc gtggtgactg cccgcgatct ccgcgatctc ccagggcagc
      481 ccccaacgct ccgccaacat tcgggaggcg cgggggtgca aagaacagac gtctccccac
      541 atcggcgcta gaccgggagc atcgtcgcct tcgacgtggg ccacggcgag caacaagccc
      601 gcatacccta catcgtgcaa cagcccgccc aaaaacgcgg tgtcggcgtc taggtgcgcg
      661 cgccgcgcga tcagcttggc gacgtacgcc gttacggtgc tatgtcgcgc taccgtttgc
      721 gccgtgtccg cgtactcgcg catcgcaaag accccctgtc gcaacgccgc ttcgaagacc
      781 gcgtctcgca cgccgcgcac cccgattcgg accacggcct cccgaagtga acggatgcgg
      841 gcccgcccgg cgtagaacgg cgagctcacg aggcgcaaca cggtgccggc cagcatcgcg
      901 tcgcgttcga gtagagccac tacttcgccg agatccggat ccggctgacc cgagatcgcc
      961 atcagctcca aggcgactcc cggcagagct ggcgggcggt atgtcgggtc atcgaagacc
     1021 aacagcaggt tgtgacaaag cgccgcggat acgagatcgg tcatgatgcc acccacgtca
     1081 cagcgcgact ttgcggagct cggaacgacc tttacctgac gtcgacgacc aagcccccct
     1141 cgcgcccgag gtgatttgcg aagcgctgaa gcacgcctcg cgacggcaag agctgccctg
     1201 cggcagcctc gcctcgagcg aactacctga cctagattac cggtagaaac ccgagaaact
     1261 taacgccgag cgcggccgga tcgtgccccc cgtcacctcg cctgaagctc tcgttagcac
     1321 gcgacccgtt cacgatgtgc cgagcgcagc cgcggagcat tggctgaggc aggaatgccg
     1381 ggtacgcttc tgggcccgca gtgccgactc cgcccaatcc tcccactgga ccgcatgtgg
     1441 atctcgacga cgatccgctg ttgccttggc tcgtcgcaac gcggagcggc gatcggagcg
     1501 ccgagcacga gctcttgctc cgactcgccc ccatcacgtt gggcttggcc cggcgactgc
     1561 ttccggacac tcgaacgcgc ccggacaacc ccaaggatca gtccgcgctc gcctttcagg
     1621 cgttgctgct cgttttgcgt gccctcccca gcttgcgggg tgaagagcgc acctcgagag
     1681 cggtggcaaa gctgacgctc gaagccgcac gccgcttccc cggtacgtcg accatcgctg
     1741 aaacggaggc gttgagcgcg gcccgcgagc tcgagcaggc tacggcgcgc ggccgcgatg
     1801 atcgcctggt gcatgagctc gcggaccgag cgtttcggga cgactcctcg atattggtca
     1861 gccaagtgat cgtcaaacct ggtgaggcca cgcgtcggtc gcggttgcgg ctcgcgttgg
     1921 ccgcgctcgg actgctaaca gcagcctggc ttggttacct gctcggcaca cgccgcaatc
     1981 gctgaagccg cccgagctgc agttacgcga acggccaagg tgagacgtgg aaccaacgct
     2041 gatgttgcga caaaatcccc agcaacagcg ccattgccag cacgaacggc agcgcgagtt
     2101 cgcgccaaac caagcgattg cgcccgcgaa tcaaggccgc gaacggtaaa ttcgaggtct
     2161 tctcacgaag cgcttcgaac tcgagtggct gcgcccgccg ccgcttctcg tcgatatctc
     2221 gggtgccttg tagcgcggtc aagccgatgc tcccgaaaaa cagcagcccc gtgagcgtgc
     2281 cgctcaccag caagtgcacg gtcgaccaca gcgcggcggc ccacaagaaa ggatgccgag
     2341 tgatgcgcag aactcccctc gcctcgactt tgcgagcgag caaagcttcc ccacctacgg
     2401 aggtggggtt tgggacgcta aaggcaccca ccaacaagaa cagggcgaac ggcatgctcg
     2461 aaatgggcag ccatctaaac cagtcgggca ccgaccagag tgggacgcac ggagccgcgc
     2521 cgtaagcgtg caccaggaag acgagcgtca cgaccgagag caaggagaat acccctcgat
     2581 aggccttctc cccaatccgc gcgatcagaa cgcgacgcag ccccgaaccc gcgatgcccg
     2641 catgcaacgc gaaccacaat gcggcagcca aacccagctg aatcatggcg cgattcagac
     2701 gctcagagcg cgctcgatgg ccgcaatgag cttgggatct tcgggttcga cggacgaggg
     2761 aaagtattca aggacgtccc cgctccgact caccaagtac ttgccgaaat tccaagccgg
     2821 agccacgccc gcagcccgtt ccaagcggcc atacaaatcc gactggccgg cgccgggttt
     2881 cgcgtgcacc ttctcgaata gggggaaggt cacctcgtaa cgcgactcgc agaaggttct
     2941 gatttcttct gggctccccg actcctgggc tccaaagtcg ttgcaaggaa aaccgagcac
     3001 ggtgaagcct cgctcctgaa agcgccgctg cagccgctcc aggcccgcgt actgcggcgt
     3061 gtacccgcag gcgctcgcga ggttcacgat caggctgacc tggtttcgat agcgctcgag
     3121 cgaaactggg gtgccttcga gcgtcttggt ctgcaaatcg taaatggtca tgtctctcct
     3181 cgtcgatttc tcagcggcat tctaagacca ccaacccaga acggaagcgg cgacccgcga
     3241 acgagatggc ttccagcggg ccgaggagca tggacgggcg aactgaaatc tccggcggcg
     3301 cgagaaacca accggcaatg ctctgcctct cagcgggatc gcacaggcgt ttttttcgac
     3361 gtgtcgaggc gttcagccgg gggcaggtcg ttctgggagc caggcgctga ccgtaatttc
     3421 ccgggccgaa gcaggcggcg attgtctcgt tcaacacggg ccgggtgaag tcaaagctgc
     3481 tattcgaagg ggattgggcg gccttgaacc aacccgcaat aaacaagccg gcagcccgct
     3541 tacgatcgac ggccctatat gagtgatact gcttggcgaa ttttgattgt cgacgagcag
     3601 ccggagcagc gtgcgcactg gcgtcgcctc ctactcgaag gttcagagcg ggagtaccag
     3661 tttacggaag tcgaatcggc gcaggccgcg tgcaagttgt tcacggcggt gaacccccaa
     3721 ttcacctgca tcatcgcgcc agacgatacc gcgagcagtg acggcccgtt gctgttgaat
     3781 cgcgagctca cgcgcgcccc ggtcgtggtc gtcaccacca gtcgttccca ccggcacgcc
     3841 aaggaccttc tggcgcgcgg agcggcagac tacgtcgggg cgcactggct cgggcctgaa
     3901 gcgctaacgc gcgccgtcga acgtgcggtc gagacctggg atctcgaagc ggcgctaacc
     3961 cacgcgcggc agcgcgaatg ccggtcgagc tcgggacccg gtttggtcga cgcatgcaac
     4021 gagaggggcc cggccagcct ctcgcgtgcg ctcgaggaaa gagagaaact gctgggtctg
     4081 gcgctggaag ctacggagac gggtctctgg acgtggaacc tcgccaccgg ccatgtgatg
     4141 tggtcaccgg agtgctatcg gattcatgga ctgacgcccg acgagttcgt ggggtcggcc
     4201 gccggcttct tcgagctcgt gcaccccgaa gaccgagcgc gcgtcgaaac gaccgttcac
     4261 tcggcaattc gagatcggca cttgtacgaa tgcgtatttc gcattctgcg gcccgacgcg
     4321 agcgtgctct gggtcgagaa tcgcggccgg gcgagctacg acaccgaggg tcaggcgacc
     4381 cggatgctgg gcaccattcg cgacgtaacc cagcgccggc gcacggaaga agcggcgggg
     4441 cgcgccgaag ccctagtcca agcggtgatc gatgggaccg aggcgttggt tttcgccaaa
     4501 gatctcgagg gacgctactt cctgtacaac cgtcagtggc tcgagtttgc agggatgacc
     4561 gccgaagagg cctgcggcat caccgacgat cgcgtattcg gggccgctgc agcccagctg
     4621 cgtgagaatg atcgacgggt gatcgagtcg gcagcgcccc tgagaaccga ggagcgagcg
     4681 acgctgaggg gccgagagtg cgtctatctc tcgcacaagt tcccgctgcg cgatccgaac
     4741 ggaaacacct atggggtttg cggcatatcg gtcgacatca ccgaactcga gaagctccag
     4801 cagagtctct cggacagcaa acgcgaactc cagactctgg cggacaacag ccccgatctg
     4861 ctgacgcgct tcgacaaaca actgcgacat gtattcgtca acgcagccgc ggaaaagctc
     4921 accggcctgc cgagcgctgc gtttctgaac aaaacgaatc gtgagctggg aatgccgccc
     4981 gagctctgca aactttggga ggcggggcta cagcgcgtgt tccagaccgc ggaacccgag
     5041 tctctcaagt tcgttttcga ctctggcaat ggtctaaagc acttcgaatc tcggctcgtg
     5101 ccggagttgg gcctgctcgg cgaagtgacc cacgtactcg gcctgacgcg agatgtgacc
     5161 gatcgtcacc gctacgaaga agcgttgctg ctcgccgacc gtcgcaaaga ccagttcctg
     5221 gccacgctgg cacacgagct ccgcaatcca ctcgcaccgt tgcgcagcgg gctcgacatc
     5281 ctcagagccg agaaccgcta tcccgattcg ggccagaaga cgctgcagat catggatcgc
     5341 cagctcaacc acctggtgcg cctcgtcgat gacctgctag atgtatcgag gattgcgagc
     5401 ggcaaagttg tgcttcggcc cgagctcgtc tcgatgcagt gggtcgtgaa cgcagccctg
     5461 gaggcaacgc aaccttgcct ggatgcggca cgtcaccacg tacacctcga tttgccgggt
     5521 gatgcggtct gggttcgggg tgatccgact cgcttggccc aagtcgccac caatctgctc
     5581 acgaatgccg ctaagtacac ccccgacggt ggacgcattc gtgtttcgtt gagcgccgga
     5641 gccgatacgg cccggctcat cgtgcaggac tccgggatcg gtattccaga gcgcatgctc
     5701 gcccacgtat tcgagctgtt tgcccaagtg aaccgagaac aaaagcgcaa gcaagatggg
     5761 ctcgggatcg ggctcgcgct cgtgaagagt ctggtggcaa tgcatggcgg atcgatcgcc
     5821 gtccatagtg cgggagaagg attggggagt acgttcacgc tggagctacc gcgggctctc
     5881 gagcgtcagg gcccgcagga acagccggcg cggctcgagc cagaagccac gtcctttccg
     5941 cctcgtcgtg tgctcgtcgt cgatgacaac gaggatgcgg cagagctcct cggctccgca
     6001 ctcgagctca aagggcaaga cgttcgcact gcccatgacg gccgctcggc cctgctcact
     6061 gcccgtcagt tccgaccaga tatcatattt ctggacatcg gtttacccga tatggacggt
     6121 tgcgaagtcg ccaaacagct gcgcgcgcaa ccggagtttc gagaggccct gctgatcgcg
     6181 ctgacgggct gggggagcga cgaagacaag cggcgcaccg cctccgccgg gttcgatctt
     6241 catctgacca aacccgttga agcccactcg gtcgcgcgcg tcctcagtca ccccacagag
     6301 tccgggccga gcacgtccgc accggaggtg agccgagagc gcgagcaacc gcccagttac
     6361 ggccaggaag cttgaatgcc ggctcagacc acgggtaagc gccgattgcc aaacggcagc
     6421 gcctcctgcc ccgtatctat tcgcaagacc tgatgagact ccggcaattc gagcactgaa
     6481 gaatcgacgt tggcgcgcgg cggggtcaac ggggccggcc actcgcaaac gtcagggggc
     6541 aaaggcccgg gtaaggtggg ttgttccgtg cgctcgaaca gctggaaatc gtgtcgcccg
     6601 tcgagttcga gcggcaaccc gagcgccggc cctccgcgct tgaaccagct ccgcgcggcc
     6661 tccacgttcc taatcaacac gtaatgttgc ggatcgctgc gggccgcgag caagaactgc
     6721 tcttgcatcc gttcgatttg accggagcgc tgcgcgatca cagccagcat gttgcgcgcc
     6781 aaacccggcg ccgcgaagcc caattcgaga gcacgctcgg cgtgcaaacg cgcttcgggc
     6841 agcttctctt cgcgcaggta ggcccccgcc aaatccagat gcgatggggc atggtcaccc
     6901 aagcgctcga ggatgccgcg gcactcgtcg actccgggtt gataggcctc cctgagcccg
     6961 gagttgcgag caaaccaatc cgtcatcagg cgagtgtccg cgtcgttcgc gtcgaacggc
     7021 accttcagct cttgaaaggc tt
//
LOCUS       DASTKU010000768        10017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1938776, whole genome shotgun sequence.
ACCESSION   DASTKU010000768 DASTKU010000000
VERSION     DASTKU010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10017
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1938776"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..585
                     /locus_tag="VFQ61_26765"
     CDS             250..585
                     /locus_tag="VFQ61_26765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958138.1"
                     /translation="MMPWSEESGPPSARGSALLENVAALRAAASEPVVSAEGLDHGTT
                     GVTLVAPEAPTLEAPPPSRIVVEGQSTARDYGAASSDARASDAEAAPESETIQGLDRR
                     DPAARREAN"
     gene            complement(868..1851)
                     /locus_tag="VFQ61_26770"
     CDS             complement(868..1851)
                     /locus_tag="VFQ61_26770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958139.1"
                     /translation="MTRSPDDASREPLDFEERRFMRLLLAAAREEEPDPELREDVLRS
                     LSLRPKVPIQNELGRSGLTHIWLQSWALRLGVGAAALGAAAAWLFYMRANRAEPASVQ
                     AELPGAVRSAREERASAASRLGQVQLNPSAPCSERRVAQGERGLIDDFEDGNADVLPY
                     EGRRGFWSWVQDSDFPRRSPELVPVLRPDPSQGNRFALHVQGKRLLDWGASIEFTFTP
                     SCYDARVYRGLSFQARGPGRIYVALREVSVIPPSGGGTCKDDCHNPHVHKVDLDGTWK
                     RYTVSFSELTQRGYGHRGLDPSQLNSVAFFVRSEDTPYDVWIDDVTFEREP"
     gene            complement(1848..2468)
                     /locus_tag="VFQ61_26775"
     CDS             complement(1848..2468)
                     /locus_tag="VFQ61_26775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9958140.1"
                     /translation="MTQTLESRVIPFSLRFGRSPESSTLSAARPEPSLRVDQRLENSV
                     RNYLTHVWRVLRRSGLREADADDAAQDVFWILAQKLADVPEANERAFLTATALRVAAD
                     RRRLRWNRAATEPMDPEFASADALPDEAIDRERARRLLDRALLTLDSHERDVFVLFEI
                     EQMSRTEIARVLAIPEGTVASRLRRARTQFEAAVRRIHASKLVRQP"
     gene            complement(2605..3225)
                     /locus_tag="VFQ61_26780"
     CDS             complement(2605..3225)
                     /locus_tag="VFQ61_26780"
                     /inference="COORDINATES: protein motif:HMM:NF025948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958141.1"
                     /translation="MKALHRPDLYAWSRFDEARNMDFNSWLWVRPQGNVVIDPLPLTP
                     HEQSRLEQLGGVRYVVVTNSDHVRDAQNLAAWAGAQISGPAAERASFPLTCQRWLADE
                     EEVVPGLRTLELNGSKTPGELALVLEDTTLITGDLVRAPRAGALDCLPDSKLANRAEA
                     VRSLQRLAALGRVETVLTGDGWPVFRFGAQALRELAARMSSASEKQ"
     gene            complement(3245..3826)
                     /locus_tag="VFQ61_26785"
     CDS             complement(3245..3826)
                     /locus_tag="VFQ61_26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HET9958142.1"
                     /translation="MAELVSDAQHHDEVLLRALREARVSLWIASANLKDIRLEAPIGT
                     RARARGRYVSVTEFLQELVSRGVDVRILHADKMSGPFARSLGELRGMPSAGFEVRACP
                     RVHLKLIIVDGRFLYLGSANFTGAGLGARAEGRRNFELGVVTDDHLLLDAVQLRFDRI
                     WTGQDCGACRLRANCPKPLDGLQTPRRLRDRAR"
     gene            complement(4075..5019)
                     /locus_tag="VFQ61_26790"
     CDS             complement(4075..5019)
                     /locus_tag="VFQ61_26790"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HET9958143.1"
                     /translation="MATQGFGRSRERELGAVVAPDAKGPRVAESASPRCLSGSVWRSR
                     VHWMLLACSAAWAAVACEDSSSPAGVSGGQSSASGGASVGGASHTGGRQSTGGSDNGG
                     ASTAVSGANTGADPGVSTGGRIVETTGGGAGGTSAGGQGGGESGAGGEGRPSIPTLQS
                     AQALYSNWHRATEEPQNISSSIFALCRLPSMAEQAFVESEHGDNLYLLDWLNDEAYAG
                     AAVREREPFPTGAAIVKEKLRWNGDAGFELAALGIMIKHEPGYDSEHGDWEFGYWESG
                     LDAPQSDAETATYCGGCHATSPTDFAFLDQRWRQLGLK"
     gene            complement(5127..6155)
                     /locus_tag="VFQ61_26795"
     CDS             complement(5127..6155)
                     /locus_tag="VFQ61_26795"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9958144.1"
                     /translation="MSETGPSASSTIAASAQELARALRSGPLAELGLVRRDLRRVRKL
                     LSDSVKQLQKSFYDLAGQVQKQREQLDGLLATVDKSNDRAPSAAQKTIRTFINDITPL
                     LESLTALLTHLAAQGASGAQRVESMTKELNETFALLGQIESIARQTDIISLNAAIEAS
                     RAGEKGHGFAVLAKEVRQLAAFSKTLNDQIAVQLKKTARAMGEVQRLLVDSSTRDSDA
                     TESAEARIQSSLADLAELDRSVGKGLEAVSQIAHRTSESVGAAVRCLQFEDIVGQLLD
                     SVQGRIERVERALGSFERLVTSVSAPQPQLASELEAQLDELSEIFEEEHDCPVHQTSM
                     AVGQVELF"
     gene            6387..8126
                     /locus_tag="VFQ61_26800"
     CDS             6387..8126
                     /locus_tag="VFQ61_26800"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9958145.1"
                     /translation="MDWFKNLSTLAKLMSSFALMGALMGFVGYQGTASAERIKDLLDR
                     VYEKDLIGSVAVKEVALELAMIRTYVRQGVLDEQLADMQRDGQLAEESFRKLDTAIAD
                     AEKTFMTAEGKALIAQISDLVPELRTGIKQVFQLTMDQRNKEAMQRLKELKPTADQIF
                     ALAERATAMKEKRAEEAFAEASAAFSNGRQVVLGITLVSAVMAMLLGYAISKMISAPL
                     TEASGVLSKVAERDLSVRMTGEAHAEFRVIKESLNGALDMLSRALTDVRVAAEQVADT
                     SQQLSSASEEISSGAQEQASSLEETAATLEEITSTVKQNADNARQANQLAANSRGSAE
                     KGGQVVGSAVSAMGEINSSSKRIADIITTIDEIAFQTNLLALNAAVEAARAGEQGRGF
                     AVVAAEVRNLAQHSATAAKEIKGLIQDSVRKVENGSELVNRSGQTLTEIVSGVKRVTD
                     IVAEIAAASQEQASGIDQVSKAMAQMDQVTQANSSQTEEMSSTAEELASTAERLRELL
                     STFRLDLRDEQSRPTQAVVPPKLPMSKPRAQIRRAPPARSASMLSAHVAGTGTNGHAA
                     PVLEADSDGFVEF"
     gene            8129..8605
                     /locus_tag="VFQ61_26805"
     CDS             8129..8605
                     /locus_tag="VFQ61_26805"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9958146.1"
                     /translation="MSLAQGTPDARTQTRQYLTFQLAEELYATEILAVQEIRGYSGVT
                     PIPHSPPHIKGVMNLRGAVIPILDLRLRFAMEPLEYNKFNVIIVFSFGEKAFGVIVDA
                     VSDVLDVTAAEVRPPPEFTARSDSRFISGMATVGEKLVVLLDVARLMQEEVATIAA"
     gene            8659..9024
                     /locus_tag="VFQ61_26810"
     CDS             8659..9024
                     /locus_tag="VFQ61_26810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9958147.1"
                     /translation="MPKTALVVDDSASMRQMISYVLTQAGFVVAEGADGKQGLSKLES
                     QKFDIIITDLNMPVMDGIEFIKQLRSQPKLKYTPVLMLTTESLDSKKQAGKAAGATGW
                     LVKPFNPQQMLQVIAKVLP"
     gene            9031..>10017
                     /locus_tag="VFQ61_26815"
     CDS             9031..>10017
                     /locus_tag="VFQ61_26815"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HET9958148.1"
                     /translation="MQRAVATQSPDAGYEFALTDADFEAFRELILQRSGISLAATKRS
                     LLCGRLARRLRHFGFSTFTQYYQYVTRQDRSGSELRELINRITTNKTEFFRENHHFEF
                     LTREVLTPLRTQAANGGPRRLRIWSAGCSTGEEPYSIAITIAEALPLDGWDVRILASD
                     IDTEVLQHAAAGEYPTSSLERVPAALRKRWFTSAATHSRQFRIHPELRKLVSFRQINF
                     ISEPWPIQSQFDVIFCRNVTIYFNRDVQRRLYERFARLLRPDGFLVAGHSENLHWLSH
                     LFTPQGSTVYRVVGTRAAATQTPKAQAVVGARTNPSAGPPPGAKSPPSVEPDA"
BASE COUNT         1949 a         3270 c         3031 g         1767 t
ORIGIN      
        1 gtctctacgg agccgtcgcg ttgggggcga tcccgcgcgc cgactttcag cgacgcagcg
       61 aactccggac caccgagctc ggttgggccc ggctcggttg ggcccggctc ggttgagccc
      121 ggctctttca acgcggcgtc aatcagcccc aactccccac gattgaaccc ggtgggaccg
      181 agcgcggtag cagcgagcca cctgagtccg ggcgccccta tggttggcgc cgggagcgag
      241 ctgagcgcga tgatgccctg gagtgaagag tcagggccgc ccagcgctcg aggctcggcc
      301 ctgctcgaga atgttgccgc gcttcgggcc gccgcgtccg agccggtcgt gagcgcggag
      361 gggctggatc acggtacgac gggcgtcacc ttggtggccc ccgaggctcc gacgctcgaa
      421 gcgccccctc catcacgcat cgtggtcgaa ggccaatcca cggcgcggga ttacggcgcg
      481 gcttcgtcag acgcgcgagc ttccgacgcc gaggccgccc ctgagagcga aacgatccag
      541 ggattggatc gacgcgaccc cgcagcccga cgagaagcaa actaacgggt aacacgccgc
      601 caggtagcag ctccgcgagt gcccgcgtta cttggcgccg cgggcgcggt acagggtgag
      661 agtgagagtg caccgactgc gctaaagcgc ggggtatgcc gagtttcgcg ctcgttattt
      721 gggggcggca tacggcaagg ccgatctact cagccgcaac ttcgcgtgga ccggtccaga
      781 ggccctgacc gtcgtacgcc catggtgatt cgaggagtgc tggtgccgct ctcgcctccg
      841 aacgccggcg tgaagcgaga gataacttta gggctcgcgc tcgaacgtca cgtcgtcaat
      901 ccacacgtcg tacggggtgt cctcggatcg cacgaaaaac gcgacgctgt tcagctggct
      961 cgggtccagc ccgcgatgac cgtagccacg ttgcgtgagc tcgctgaaag aaacggtata
     1021 gcgtttccaa gttccgtcca ggtcgacctt gtggacgtga ggattgtgac aatcgtcttt
     1081 gcatgtaccg ccccccgaag gcgggatcac gctgacctcg cgcagcgcaa catagatgcg
     1141 acccggcccc cgcgcctgga aagacagccc acggtaaacg cgcgcgtcat aacagctagg
     1201 agtgaaggta aactcgatgg aggcgcccca atcgagcaag cgtttaccct gtacgtgcaa
     1261 ggcaaagcgg ttaccttgac tcgggtcggg gcgcaaaacc ggcaccagtt cgggcgaacg
     1321 tcgtgggaaa tcgctgtcct gaacccagct ccaaaatccg cgacgcccct cgtacggcaa
     1381 cacgtccgcg ttgccgtctt cgaagtcgtc gatcaggcct cgctcgcctt gcgcgactcg
     1441 gcgttcgctg cacggtgcgc tcggattgag ctgaacctga ccaagccgag acgcggcgga
     1501 ggcgcgttct tcccgtgcag atcgaaccgc acccggcagc tctgcctgca cgctcgcggg
     1561 ctcggcgcgg ttcgcgcgca tgtaaaacaa ccaagccgca gccgccccta gcgcggccgc
     1621 ccctacgccc aaccgcaaag cccaggactg cagccagata tgtgtgagtc cgctccggcc
     1681 aagttcgttc tgaatcggca cttttggacg cagcgagagg ctcctcagca catcctcgcg
     1741 aagctcgggg tcgggctcct cttcgcgggc agctgcgagc aagagtcgca tgaaccgacg
     1801 ctcttcgaaa tcgagaggtt cccgggacgc atcgtcggga gatcgagtca tggctgcctc
     1861 acgagcttgc tcgcgtgaat gcggcgaacg gcagcctcga attgtgtgcg ggcacgacgc
     1921 aagcgggaag ccaccgtgcc ttccgggatg gctagaactc gagcaatttc ggtgcggctc
     1981 atttgctcga tttcgaagag aacgaacaca tcgcgttcat gactatccaa ggtcagcaag
     2041 gcgcgatcca acaagcgtcg ggcgcgctcc cgatcgatcg cttcgtctgg tagcgcgtca
     2101 gcgctggcga actcggggtc catcggttcg gtcgcagctc ggttccagcg caaacgtcga
     2161 cgatccgcgg cgacccgcag cgcagtggcc gtgagaaagg cgcgttcgtt cgcctcggga
     2221 acgtccgcaa gcttctgcgc caaaatccag aacacgtctt gagcggcgtc gtcggcatcg
     2281 gcctcgcgta ggccggatcg tctcagcact cgccagacgt gcgtcagata gttgcgcacc
     2341 gagttctcga gccgctggtc gacccgcagg ctcggttcgg gacgcgcagc ggagagcgtc
     2401 gagctctctg ggctgcgccc gaatcgaagg gagaagggga tcacgcggct ttccaacgtc
     2461 tgtgtcatgt cgacaaaggg cgattcggat cgtgaaaacg aagcgcaccg acgaatgacc
     2521 gattccgctc attctcgaga tcggaaagtg gcggagccaa gcccctgaga aggccgctct
     2581 actcgagcgc ctcggaggtc gtctctactg cttttccgat gccgagctca tgcgcgccgc
     2641 cagctctcgt aacgcctgtg cgccaaagcg gaagacgggc cagccgtccc cggtgagtac
     2701 ggtttctacc cgaccgaggg cggccagacg ttgcagcgat cgcaccgcct cggctcgatt
     2761 ggcgagcttg ctgtcgggca agcaatccaa agctccggcg cgcggagcgc gcaccagatc
     2821 ccccgtaatc agagtcgtgt cctcgagtac gagcgccagc tcacctgggg ttttcgaacc
     2881 attcaactcc agcgtgcgca gacccggcac tacttcttct tcgtccgcga gccaacgctg
     2941 acaagtcaac ggaaagctgg cccgttccgc ggcgggtccg ctaatttgcg cgcccgccca
     3001 cgccgccaga ttctgcgcgt cacgcacgtg gtccgagttc gtgaccacga cataacgaac
     3061 tcctccgagc tgctcgagcc gagattgctc gtgcggcgtc aggggcagcg ggtcgatgac
     3121 cacgtttcct tgcggtcgca cccatagcca gctgttgaaa tccatgttcc gcgcttcgtc
     3181 gaagcgcgac caggcgtaca gatccggtcg gtgcaaagcc ttcatgacgg gagccgaagc
     3241 taacttaacg agctctgtcg cgcaaacgcc gcggcgtctg cagcccatcg agaggcttcg
     3301 ggcaattcgc gcgcagccgg caggcgccac aatcttgccc ggtccaaatt cgatcgaagc
     3361 gtagctgcac ggcgtcgagc agcaggtggt cgtccgtaac cacaccaagc tcgaagttgc
     3421 ggcggccttc tgcccgcgca ccgagcccgg ccccagtgaa attggcgctg cctaagtaca
     3481 agaaacgccc atctacgatg atgagcttga gatgaacgcg cgggcaggca cgcacttcga
     3541 agccagccga aggcatcccg cgcagctcgc ccagggagcg cgcaaaaggt ccggacatct
     3601 tgtccgcatg caagatgcga acatcgacgc cgcgtgagac cagctcttgg aggaactcgg
     3661 tcaccgaaac gtagcgaccg cgtgcgcgcg cccgcgtgcc gatcggcgcc tccaggcgga
     3721 tgtccttgag gttggcggag gcgatccaga gcgaaacccg cgcttcacgc agggctctca
     3781 gcaacacctc atcgtgatgc tgcgcgtcgc tgacgagctc ggccatcccg gcgggtcgag
     3841 ctcgctctgt atttgcgcgc gagcgcccta gcttcgcgcg cgagcgctct gctgttgcgc
     3901 gagctggcac ggctttgtca tcggacgcgg ttacggcgcg gcgcggcgaa actccggaag
     3961 cgtcactttc cccagctgcc cggggtttct tgcgaggaga gcgcgctttt tccagcgctg
     4021 agaggaaacg ttaaatggct gcgtctgtca agctgctcct gaacgcccag tggctcactt
     4081 caggccgagc tgccgccagc gttgatcgag gaaggcaaag tcagtgggcg aggtcgcgtg
     4141 gcagccaccg caataagtcg ccgtctccgc atcggattgc ggagcgtcca aacctgactc
     4201 ccaatatcca aactcccaat ctccatgttc cgaatcgtag ccaggctcat gcttgatcat
     4261 gatccccagc gcggcgagct cgaaaccagc gtcgccgttc cagcgtagtt tctccttcac
     4321 gatcgcggcc ccggtcggga aaggttcgcg ctcgcgtacc gcggcgccgg catacgcttc
     4381 gtcattgagc caatccaaga gataaagatt gtcgccgtgc tcactctcga cgaatgcctg
     4441 ctcggccatc gaaggcaatc gacacaacgc gaagatgctg gaagaaatgt tctgaggttc
     4501 ttccgtcgcg cggtgccagt tcgaatacag agcctgggcg ctctgcagcg tcggaatcga
     4561 gggcctccct tcaccacccg cgccgctttc tcccccgccc tgcccgccgg cagaagtacc
     4621 gccagctccc ccaccagtgg tctcgacgat gcgacctccg gtgctgacac ccgggtctgc
     4681 gcccgtgttc gctccgctga cagccgtcga agctcctcca ttatcggaac ctccggtcga
     4741 ctgccttccg ccggtgtggg atgcaccgcc cacgctggcg cctccgctgg ccgatgattg
     4801 gccgccggaa actcctgccg ggctactcga gtcctcacac gcaaccgctg cccaagcggc
     4861 actgcacgcc agcagcatcc aatgcacgcg cgatctccac accgacccag agagacagcg
     4921 gggtgacgca gactcggcga cgcgcggtcc cttcgcatcg ggcgcgacca ctgctcccaa
     4981 ctcacgttct ctcgaacgcc caaaaccctg tgtagccatg agggttccaa gctagcatgc
     5041 ccgagtacct cggcggggac gagcattcag ccactccgac gcggccgtcc aaatattgtc
     5101 accaactcgg atgcgagcgc agaattctag aacagctcga cctgcccgac cgccatcgac
     5161 gtctgatgta ccggacagtc gtgctcttcc tcgaagatct cgctcaactc gtcgagctgc
     5221 gcttcgagtt cacttgccag ctgtggctga ggcgccgaga cgctcgtgac cagccgctcg
     5281 aacgagccga gggcccgctc cacccgctca atccggccct gaacgctgtc tagaagctga
     5341 ccgacgatat cctcgaactg cagacagcgt accgccgccc cgacgctctc actcgtgcga
     5401 tgcgcgatct gcgacaccgc ctccaggccc ttaccgaccg aacgatcgag ctccgccaaa
     5461 tcagccaagc tgctctgaat gcgagcttcc gcggattcgg tcgcgtcact atcgcgagtg
     5521 gacgagtcca cgagcaggcg ctgaacctcc cccatcgcgc gcgccgtctt cttgagttgc
     5581 accgcgatct ggtcattgag tgtcttggag aaggccgcga gctgtcgaac ctccttcgcc
     5641 aaaacagcga atccgtgccc cttttcaccc gcgcgggagg cttcgatggc tgcgttcagg
     5701 gagatgatgt cggtctgtcg ggcaatactc tcgatttggc cgagtagcgc gaaagtctcg
     5761 ttgagctctt tggtcatgct ctcgacgcgc tgcgcgccac tcgccccctg cgcggccaga
     5821 tgggtgagaa gggcggtcaa tgactccaac agcggcgtga tgtcattgat gaaggttcgt
     5881 atcgtcttct gggccgcgct tggagctcgg tcgttcgatt tgtcgacggt tgcgagcaga
     5941 ccatccagct gctcgcgctg tttctgcact tgccccgcga gatcgtagaa gctcttctgc
     6001 agctgcttca ccgaatcaga gagcaacttg cgaactcggc gcaagtcgcg gcgaaccagg
     6061 ccaagctccg cgagcggccc actccgaagt gcgcgcgcca gctcctgcgc gctggcggcg
     6121 atggtggacg aagcagatgg gccagtctca gacatgcgac aagctatcga acggctgttg
     6181 tccgggatct cttcagcgtc gaattacgtc attcaacgct gtcatgacac ttggctccct
     6241 tttcgagatc ctcggagcag attgcctcaa cgaactgctt agacagccgt tcggaactgg
     6301 caacgaaggc tggtttagcc gcatttcgcg cgaaaccggg cgggagacac tccttcgtat
     6361 tgattcagca ccacgagtga attgccatgg attggttcaa gaacctgagc acgctagcga
     6421 agttgatgtc gagcttcgcg ctgatgggcg ctttgatggg cttcgtcggt taccagggca
     6481 cggccagcgc cgagcgcatc aaagatttgc tagatcgtgt ttacgagaag gacctgattg
     6541 gatcagtggc ggtcaaagag gtcgccctcg aactcgcgat gattcgaacc tatgttcgac
     6601 aaggcgtgct ggacgaacaa ctcgccgaca tgcagcgcga cgggcagctg gcagaagagt
     6661 ccttccgcaa actggatact gcgatcgcgg acgcggaaaa gaccttcatg accgccgaag
     6721 gcaaggcgct cattgcgcag atctcggatc tggttccgga gttgcgtacc ggcataaagc
     6781 aggtgttcca gctaacgatg gaccagcgga acaaggaggc catgcagcgc ctcaaggagc
     6841 tgaaacccac cgccgatcag atcttcgccc tcgcggagcg ggctacggcg atgaaggaaa
     6901 agcgcgccga agaagcgttc gcggaggcga gcgccgcctt cagcaacggc cgtcaagtag
     6961 tactcgggat taccttggtc tccgctgtca tggccatgct gctgggatac gcgatctcca
     7021 agatgatctc ggcgccgttg acggaggcct cgggggtgct ttcgaaggtg gccgagcgcg
     7081 acttgagcgt tcgcatgacg ggcgaagccc acgccgaatt ccgcgtcatc aaggaatctt
     7141 tgaacggggc gctggacatg ctcagccgag cgctgactga cgtgcgggtg gccgccgagc
     7201 aagtcgcgga cacatcgcaa cagctctcct ccgcttccga ggagatctcg agcggcgcgc
     7261 aagaacaagc ctccagtctg gaggaaaccg ccgccacgct cgaagagatc acctccaccg
     7321 tgaagcagaa cgccgacaac gcgcggcaag ccaatcaact cgccgcgaac tcgcgtggct
     7381 ctgcggaaaa gggcggacag gtagttgggt ccgccgtatc ggcgatgggc gaaatcaact
     7441 cctcctccaa gcgcattgcc gacatcatca cgacaatcga tgagatcgcc ttccaaacca
     7501 acctactcgc gctgaacgcc gcggtcgaag ccgcgcgcgc cggcgagcag ggccgcggct
     7561 tcgccgtcgt ggcggccgag gtgcggaacc tcgcgcaaca cagcgcgacg gccgccaaag
     7621 aaatcaaggg cttgatccaa gactcggtac gtaaggtgga gaacggttcg gagctagtga
     7681 atcgctccgg ccagaccctg accgagatcg tgagcggcgt gaagcgagtc actgacatcg
     7741 tcgcggagat cgccgccgcc agccaggaac aagcctcggg catcgaccag gtctccaagg
     7801 ccatggcgca gatggaccag gtgacgcagg ccaactcctc tcagacggaa gagatgtctt
     7861 cgacggcgga agagctcgcg agcaccgccg agcgtctacg cgagttgttg tctactttcc
     7921 ggctcgacct gcgtgacgaa cagagccgac cgactcaggc ggtcgtcccg ccaaagctgc
     7981 cgatgagcaa gccccgagcg caaattcggc gcgcaccgcc agctcgttcc gcctcgatgc
     8041 tctcggcgca cgtcgccggc acgggcacca acgggcacgc cgcaccggtg ctcgaggctg
     8101 attcagacgg attcgtggag ttctaaccat gtcgctggct caaggtactc ccgacgcccg
     8161 gacgcagact cgtcagtatc tgacgtttca gctcgcggag gagctgtatg cgaccgagat
     8221 actcgcggtg caagagatcc gcggctactc cggggtcacc ccgattccgc actccccacc
     8281 acacataaaa ggagtgatga acctacgcgg cgcggtgatt ccaatcctgg acctgcgctt
     8341 acgcttcgcg atggagccac tcgaatacaa taagttcaac gtaattatcg tattcagctt
     8401 cggcgaaaag gccttcggcg tgatcgtcga tgccgtttcc gacgtgctcg acgtgaccgc
     8461 ggccgaggtg cgcccgcctc cggaattcac ggcgcgcagt gactcgcgtt tcatctccgg
     8521 gatggccacc gtgggggaga agctagtggt cctgctcgac gtagcacgac tcatgcaaga
     8581 ggaagtcgcc accatcgccg cctgagcgcg cggaagccaa gaagctcgat ccatctattc
     8641 ctacctcgga gttacgaagt gcccaaaaca gcgttggtcg tcgacgattc agcaagcatg
     8701 cggcagatga tctcgtacgt cctcacccag gccgggttcg tcgtcgcaga aggtgccgac
     8761 ggcaagcaag gcctatccaa gctcgaatcc cagaagttcg acatcatcat caccgacctc
     8821 aacatgccag tcatggatgg catcgagttc atcaagcagc tccgctcgca acccaagttg
     8881 aagtacaccc ccgtactgat gctcaccacc gagtccctgg actcgaagaa gcaggcgggc
     8941 aaagcggccg gcgcgacggg ctggctcgtg aagccgttca atccgcagca gatgctgcag
     9001 gtcatcgcca aggttctgcc ctgacccccc atgcaacgcg cggtcgctac ccagagcccc
     9061 gacgcgggct atgaattcgc gctgacggac gccgacttcg aggccttccg cgaactcatc
     9121 ctgcaacgtt cgggtatctc gctcgctgcg accaagcgct cgctcttgtg tggccggctc
     9181 gctcgacggt tgcgccactt cggcttcagt accttcactc aatactatca gtacgtcacg
     9241 cgtcaggacc ggagcggttc ggagctgcgc gaactcatca atcgcatcac tacgaacaag
     9301 accgagttct tccgcgagaa tcatcatttc gagttcctga ctcgggaggt gctcactccg
     9361 ttacgcaccc aagctgccaa cggcggaccg cgccgactcc gcatttggag cgccggctgc
     9421 tccaccgggg aagagcctta ctcgatcgcg atcacgattg cagaagcgct gcccttggat
     9481 ggctgggatg ttcgcattct agcctcggac atagacaccg aagttttgca gcacgcggcg
     9541 gccggcgagt accccacttc cagcctcgag cgcgttcccg cggccctccg caagcgctgg
     9601 ttcaccagcg ccgcgactca ttcgagacag ttccggattc atccggagct gcggaagctc
     9661 gtgtctttcc ggcaaatcaa cttcattagt gagccgtggc cgatccagag ccaattcgac
     9721 gtcatcttct gtcgcaatgt cacgatctac ttcaaccggg acgtgcagcg ccggttgtat
     9781 gagcgattcg cgcgcttgtt gcggccggac ggatttctcg tcgcggggca ctccgagaac
     9841 ctgcactggc tgtcgcactt gttcactccg caaggaagca ccgtatatcg cgtggtcgga
     9901 acgcgcgcgg ccgcgaccca gacgcccaaa gcgcaggccg tcgttggcgc gcgaacgaat
     9961 ccgagcgcag gcccgccccc cggcgcaaag tcacccccct ccgtggagcc cgacgcc
//
LOCUS       DASTKU010000769         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_151390, whole genome shotgun sequence.
ACCESSION   DASTKU010000769 DASTKU010000000
VERSION     DASTKU010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151390"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VFQ61_26820"
     CDS             complement(<1..234)
                     /locus_tag="VFQ61_26820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9958149.1"
                     /translation="MSDRLLIVEDDPDLAENLVELCEAQGFCADVSPSAEKAMDRVGC
                     QPYRGIISDYRLPGQSGVELISALRRSGYALPVV"
     gene            complement(338..1513)
                     /locus_tag="VFQ61_26825"
     CDS             complement(338..1513)
                     /locus_tag="VFQ61_26825"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9958150.1"
                     /translation="MNLQQFDSVVRYVSFTAHDSEVLRGCLGVVRPHIVPIVDDFYDA
                     IEAHPAASQVITGGKPQIQRLKNTLIAWLETVLRGPHDFAYVESHARIGRVHVRIGLP
                     QEFMFTAMNRIRLHVKAALDKALEADRAQEARDAVDKILDIELAIMLDTYREDWNEKI
                     RSAERLATIGQLAASIGHELRNPLSTIESSLFLMAQRFKRLSVEDPQLVKHHDKIQKQ
                     VKHCAKTITNLLDLARERPPRRRRVETRTLLAQAAETVTLPPSTVLELNIPEGASLYG
                     DPDDLLHVFANLFSNAADSGPGPIHIRVTAAEHPAGTELRVQDNGPGVPTELRQKIFD
                     ALFTTKSHGTGLGLALCRRVLYAHGGELTLEPSEHGACFRVWLPEPEEAGERPQSGN"
     gene            complement(1510..1713)
                     /gene="ccoS"
                     /locus_tag="VFQ61_26830"
     CDS             complement(1510..1713)
                     /gene="ccoS"
                     /locus_tag="VFQ61_26830"
                     /inference="COORDINATES: protein motif:HMM:NF015554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HET9958151.1"
                     /translation="MSVLFVVLPLTLLISGLAVLAFSWATRRGQFDDLETPALRALQD
                     EPRLPRVVVSTHPASSGRLHDTQ"
     gene            complement(1710..>2073)
                     /locus_tag="VFQ61_26835"
     CDS             complement(1710..>2073)
                     /locus_tag="VFQ61_26835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET9958152.1"
                     /translation="RLRAQGERVVMVGDGVNDAAALSAATCGFAVHGGAEASLIAADV
                     FATESGLRPLLRAVVGSTRALHTIRRGISFSLIYNLLGVGLAVSGLLTPLWAAVLMPL
                     SSLTVVTNALKSRSFERT"
BASE COUNT          401 a          628 c          656 g          388 t
ORIGIN      
        1 caccactggc agcgcgtaac cactccgacg cagcgccgaa atcagctcga ctccgctctg
       61 tccgggcagg cgataatcgc tgatgatgcc gcgataaggc tgacagccca cccggtccat
      121 cgccttctcg gcagacgggg aaacatccgc acaaaacccc tgcgcctcgc acaattccac
      181 gaggttctcg gcgagatccg ggtcgtcttc gactatgagc aaccgatccg acatcggcag
      241 gacaggagtc tagtccgagc cgcggagacg atcgaggcga aattcacgag ctcgttccag
      301 gacgctcggc tgctttgtgg ctaacttggt cccatggcta attgcccgat tgggggcgct
      361 cgccggcttc ctcgggttcg ggcagccaga cgcggaaaca tgcgccgtgc tcgctgggtt
      421 ccaacgtgag ctcgccgccg tgcgcgtaca gaactcggcg acacaacgcc agacccaagc
      481 cagttccatg ggacttcgtc gtgaacaaag cgtcgaagat cttttgacgg agttccgtgg
      541 ggactccggg tccgttgtcc tgtacgcgca gctcggttcc agctggatgc tcggccgccg
      601 ttactcggat gtggattggg cccgggcccg agtcggctgc gttcgagaac agattcgcaa
      661 atacatggag aaggtcgtcg gggtcaccgt acagcgatgc accttcgggg atgttgagct
      721 cgagtaccgt gctagggggt agcgtgacgg tttccgctgc ttgcgcgagc agcgtacggg
      781 tttcgacacg tcggcggcgc ggcggccgct cgcgcgcgag gtccaacagg ttggtgatgg
      841 tcttggcgca gtgcttgacc tgcttttgga tcttgtcgtg gtgcttgacg agctgggggt
      901 cctcgacgct caaacgcttg aagcgctgag ccatcaggaa caacgaggat tcgatggtgc
      961 tcagcggatt gcgcagctca tgcccgatgc tcgccgcaag ctgtccgatg gtggcgagcc
     1021 gctccgcgct tcggatcttc tcgttccaat cctcgcgata ggtgtcgagc atgatcgcga
     1081 gttcgatatc caggatcttg tctacggcgt cccgtgcctc ttgggcccga tcggcttcca
     1141 acgccttgtc aagcgcggcc ttcacgtgca ggcgaatgcg attcatcgcc gtgaacatga
     1201 actcttgcgg cagaccaatg cgtacgtgaa cgcgcccaat ccgcgcgtga gactcgacat
     1261 atgcgaagtc gtgcgggcct ctcaaaaccg tctctagcca cgcgatcagg gtgttcttca
     1321 agcgttggat ctgcggcttg cctccggtga tcacctggct ggcggccggg tgagcctcga
     1381 tggcgtcgta gaaatcatcg acgatcggga cgatgtgcgg gcgcaccacg cccaagcagc
     1441 cgcgcaatac ttcgctatcg tgcgctgtga aagagacgta gcgcaccaca ctatcgaact
     1501 gttgcaggtt cattgagtgt cgtggagccg gcccgagctt gccggatggg tggacaccac
     1561 gacgcgtggc aagcgaggtt cgtcttgcag ggcgcgcagc gcgggagtct ccaaatcgtc
     1621 gaattggccg cgacgagtcg cccaggagaa ggccagtacg gccaacccgg atatcaaaag
     1681 cgtgagcggt agcactacga ataacacgct catgttcgtt cgaagctcct ggacttgagg
     1741 gcgttggtta ccacggttag ggaactcaag ggcatcagaa cagcggccca caggggggtc
     1801 aagagcccag acaccgcgag gccgaccccg agcaagttgt agatcaaaga aaacgagatg
     1861 ccgcgccgaa tggtgtggag tgctcgcgtc gaaccaacca cggcgcgtaa cagcggccgc
     1921 agtccggact ctgtggcgaa tacgtcggcg gcgatcaggc tggcttctgc gccgccatgt
     1981 acggcaaatc cacaagtagc ggccgacaac gccgcggcgt cgtttacacc atcgccgacc
     2041 atcaccaccc gctcgccctg ggcgcgcaga cgc
//
LOCUS       DASTKU010000770         5222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1043976, whole genome shotgun sequence.
ACCESSION   DASTKU010000770 DASTKU010000000
VERSION     DASTKU010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043976"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="VFQ61_26840"
     CDS             complement(<1..1588)
                     /locus_tag="VFQ61_26840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9958153.1"
                     /translation="MSILGRRQRIAGRYLLESEIGAGGMGIVWRARDERLGRDVAVKL
                     LAANAIGNDVARARLLREARAAGRLQHEGIVHVYDVGETGDGGAFLVMELVSGYPLRD
                     WMEDGKLSIDDRVEIVVGIASALQCAHELGIVHRDVKPENVLVRSSGRPVLLDFGLAK
                     PIASPLVETLGESHDIRLTGTGAVVGTPAYLAPEQVKGEPVVAPADQFSLAVLTFELL
                     TGRLPWKGTAMLEVIASMLHDTPLSVQDLAPELPVGVGPVLERAMQKDPAQRYPSVAA
                     FAEALRAFFPNVSTSRVSSLPPPAPRVRPTSTPGALRSASPETGMARRAFTPASRTPS
                     RRWLRVLAAFGAVAAALLVAGIVIYRANRAASAPAARSVEAPVALTKESVVACPLFKL
                     EGDLPDPSNGWLAAAAAALACDRVQVLLGGSPTSTLAPAELVPGLPREPTEDAPADPF
                     NKPELYESTLAAAKKLAPIYVEGRATKLAWDFSAEVTLRARDGRQLAHGEGKGEELFE
                     AISAAVRQAKPSFGDGAASEF"
     gene            complement(1667..2239)
                     /locus_tag="VFQ61_26845"
     CDS             complement(1667..2239)
                     /locus_tag="VFQ61_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9958154.1"
                     /translation="MPTPAHRIRYSWADYLALEASSNVKHEFLDGQIYGMAGGMPEHA
                     ALTAALTGLLFSQIRTGRCRVHDSDLRVRVLETGLATYPDLTIVCGARERDPEDSNAV
                     TNPTLLVEVLNRSTEEYDRGDKFEHYKCIPSLRQYVLVSYREPTVEVWTRVESGWEQA
                     VYRAGERANLASIGAELDVTELYEAAAEPN"
     gene            2330..2692
                     /gene="queD"
                     /locus_tag="VFQ61_26850"
     CDS             2330..2692
                     /gene="queD"
                     /locus_tag="VFQ61_26850"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HET9958155.1"
                     /translation="MNVRLIREMRFEAAHRLPKVPPGHKCQRLHGHSFKVELAIRGPV
                     NPDTGWFIDFGDVDLIWKPLHDQLDHNYLNEIPGLDNPTSENLARWLWDRIKPALPSL
                     ERVIVHETCDSRCEYEGD"
     gene            2855..4408
                     /locus_tag="VFQ61_26855"
     CDS             2855..4408
                     /locus_tag="VFQ61_26855"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9958156.1"
                     /translation="MQLVFGSASLRAVALVCVIALPVGCKAGKPDGSTFGSRGGAGSA
                     GNSASGGRSTHAGGAGGETGPVSPRGGSGGAASGSSGAAGADPQGGDPQGGTLGEPDG
                     TGGDSKPAAGASGAVAGECESKKSGATFCRGLQRLRCDSVGEEPVVLDTCEGQACVDG
                     ECVGECEPGQDRCALESRETCGSDGAWQAKPCPDATPLCKEGACVAPPSCSGLSESCG
                     VTGSDACCSSLAVPGGTFYRSYDGVEHGSQDAPALVADFRLDKYEVTVGRFKRFMEAW
                     TAGFRPADGSGKHSHLNQGKGLLDVGANGMNELGWDPEWAASVSVAEARECDDAQTLL
                     SNAANDSLPMNCINWYEAYAFCIWDGGFLPSEAEWNYAASGGEEQRVYPWSKPANSTN
                     IACSFANYTQEGYTVGCTTPPKGSPTWMTGSPNRVGSESPFGDGKYGHAGLAGNVAEW
                     VLDWSWTYGACTNCARTTAAANDITRLRVARGGDAQAPQSSLQNGARGVTPPSSRIYS
                     TGVRCARTL"
     gene            complement(4442..4711)
                     /locus_tag="VFQ61_26860"
     CDS             complement(4442..4711)
                     /locus_tag="VFQ61_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9958157.1"
                     /translation="MKRILCRCEDVTVEEVDHAVSLGHTDIESLKRYTGFGTGYCQGK
                     SCLVPCARRLLELGGEPGHGITPRPPLRPVSFAALASLARAPDSK"
     gene            complement(4713..>5222)
                     /locus_tag="VFQ61_26865"
     CDS             complement(4713..>5222)
                     /locus_tag="VFQ61_26865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9958158.1"
                     /translation="RLAPDFELPQVGGGKVRLSSFRGKTVILNFWSKSCPPCLEEMPS
                     IAGLAKVLRERKDIVLLTVTTDDSAEDARDTLRSVLGADAPFVTLIDPDAKVVTDLYG
                     TKLYPETWFIDPKGVIRARFDGARDWASALVVELAESLQRPYTCDVEFRGMKPAGDSA
                     FVCSDLSRN"
BASE COUNT          950 a         1701 c         1665 g          906 t
ORIGIN      
        1 gaaactccga ggccgcgccg tcgccgaagc tcggcttcgc ttggcgcaca gccgcagata
       61 tggcctcgaa tagctcttcg cccttaccct ctccatgcgc gagctgtcgc ccatctcgag
      121 cgcgcagcgt gacttccgcg ctgaagtccc aagccagctt ggtggcgcgg ccctcgacgt
      181 agatcggagc cagctttttg gctgccgcca aagtactctc gtacagctcc ggtttgttga
      241 acggatcggc cggcgcgtcc tcggtgggtt cgcgcggtag cccgggcacg agttcagcgg
      301 gcgccaatgt gctcgtagga gatccgccga gcagcacttg aacgcgatca cacgccaagg
      361 ccgccgccgc cgctgctagc cagccgttgc tcgggtcggg tagatcgccc tcgagcttga
      421 agagcggaca cgcaaccacg ctctctttcg tgagcgccac tggcgcttcg acactgcgcg
      481 cagcaggcgc gctcgcggct cggttggcgc gatagattac gattcccgca acgagcagcg
      541 cagccgccac ggcgccgaac gcggcgagca cccgcagcca tcgccggctc ggtgtgcgcg
      601 aggccggagt gaacgcgcgc cgcgccatgc cggtctcggg cgatgcagag cgcaaagcgc
      661 cgggcgtcga cgtaggccta acgcgcggag ccggcggtgg cagcgaggaa actcgactcg
      721 tcgagacatt gggaaagaac gcacgtagtg cttccgcaaa cgcggccacc gacggataac
      781 gctgcgccgg gtctttctgc atggcccgtt ccagaaccgg gcccaccccc accggcagct
      841 ccggcgcaag atcctgcacg ctgagtggcg tatcgtgcag catgctcgcg atgacctcga
      901 gcatggccgt gcctttccac ggcagccgcc ccgtgagcag ctcgaacgtg agcaccgcga
      961 gcgagaactg gtccgccggc gcgaccaccg gttcaccctt tacctgctcc ggtgcgagat
     1021 acgcgggtgt tcccaccacg gcgccggtgc cagtgaggcg gatgtcgtga ctttcgccca
     1081 acgtctccac gagcgggctc gcgatcggct tggcgagccc gaaatcgagt agaaccgggc
     1141 ggccgctgct gcgcaccagc acgttttccg gcttcacatc gcggtgcacg attccgagct
     1201 cgtgcgcgca ctgcaacgcg ctcgcgattc cgacgacgat ctccacccga tcgtcaatcg
     1261 agagcttgcc gtcctccatc cagtctcgca gcgggtagcc cgagaccagt tccatcacca
     1321 agaaggcgcc gccgtccccc gtctcgccca cgtcgtagac gtgaacgatg ccttcatgtt
     1381 gcaaccgccc cgccgcgcgg gcctccctca gcaggcgcgc gcgcgcgacg tcattgccaa
     1441 tggcatttgc cgccagcagt tttaccgcga cgtcccggcc gagccgctcg tcccgcgcac
     1501 gccaaacgat ccccattccg ccggcgccaa tttccgactc gaggagatag cgccccgcga
     1561 tgcgctgccg tctgccgaga atggacatga acgccgcttc ttactccgtt tttgtctaag
     1621 gggtatccgc cggctcaaga atcgaacttc gaggccaatc ttcagttcag ttgggctctg
     1681 ccgcggcctc gtacagctcg gtgacatcga gttcggcgcc gattgaggcc agattcgccc
     1741 gttcgccggc tcgatacacg gcttgttccc agccgctctc cacgcgggtc cacacctcga
     1801 ccgtcggttc gcgataagag acgagcacgt attgcctcag cgagggaatg catttgtagt
     1861 gctcgaattt gtcgccgcga tcgtactctt ccgtacttcg attcagcacc tcgaccagca
     1921 gcgtcgggtt ggtcacggcg ttggaatctt cgggatcccg ctcccgagcg ccacacacga
     1981 tggtgaggtc tggataagtg gcaagaccgg tttcgagcac ccgaactcgc aaatccgagt
     2041 cgtgcacccg acaccttccg gtccggattt gggaaaacaa gaggcccgtc aaagccgcgg
     2101 tcagtgccgc atgctccggc atacccccgg ccattccgta aatttggccg tccaggaact
     2161 cgtgcttcac gttgctcgag gcttcgagcg ccagatagtc cgcccagctg taacggatcc
     2221 ggtgtgctgg cgtgggcatt ggcgaagtat ggcacagcgc cggcctggcc gttcggctgg
     2281 gccgtgaact cgtacggccc cgcgctgacg gtctgctaag ctcggccgcg tgaatgttcg
     2341 gttgattcga gagatgcgct tcgaagcggc tcaccgtctg cccaaggtgc cgcccggcca
     2401 caaatgccaa cgcttgcacg ggcacagctt caaggtggag ctcgccattc gcgggccggt
     2461 gaaccctgac acaggctggt tcatcgactt cggcgatgtg gacctgattt ggaagccgct
     2521 ccacgatcag ctcgatcaca actacctgaa cgagatccct ggtctcgata acccgaccag
     2581 cgagaatctc gcacgttggt tatgggaccg catcaagcct gcgctcccct cgctcgagcg
     2641 cgtgatcgtg cacgagacgt gcgatagccg ctgcgaatac gaaggcgact aaagagcccc
     2701 tccattggga gctggtcgaa agcagccgag cgctcgcgtc ctccgacaac gaacgggtaa
     2761 cccgtaaggc tgacgtggtc gatcccatct gaatccatcc ggagaggcgc ttccatgctg
     2821 gtgtggaggt agcgggtgct ctagtgtgcc ccggatgcag ttggtgtttg ggagcgcgtc
     2881 gttgcgcgca gtcgctttgg tgtgcgtcat cgcgttgccg gtgggatgca aggccggtaa
     2941 gcccgatggg tcgacattcg gctctcgcgg aggtgccggg agcgcaggga attccgcgag
     3001 cggtggccga tccacccacg cgggtggagc tggcggggaa acgggaccgg tctcacccag
     3061 gggaggctct ggcggagctg cttccggttc gagcggtgcg gcgggggcgg atccgcaagg
     3121 gggggatccg caagggggaa cattggggga accagacggt acgggcggcg attcgaagcc
     3181 cgcagcgggt gcgtcgggag cggttgccgg tgagtgcgag agcaagaagt ccggggcaac
     3241 gttctgtcga gggctacagc ggctacgttg tgacagcgta ggtgaggaac ctgtggtcct
     3301 ggacacctgc gagggccaag cgtgcgtcga cggcgagtgc gtgggcgagt gcgagccggg
     3361 tcaagatcgc tgcgccctcg agtcgcgcga aacctgcggg agcgacggag cttggcaagc
     3421 caagccgtgc cccgatgcaa ctccgctctg taaagagggt gcctgcgtcg cacctcccag
     3481 ctgctcaggg ctcagcgaga gctgcggggt aactgggagc gatgcctgtt gctcgagctt
     3541 ggccgtgccg ggcggcacgt tctatcgaag ctacgacggc gtggaacacg gctcccaaga
     3601 tgctcccgct ctcgtcgccg acttcaggtt ggacaagtac gaggtcacgg tcggtcgctt
     3661 caagcgcttc atggaagcct ggacggctgg cttcagaccc gccgacggct ccggcaagca
     3721 tagccacctg aaccaaggaa aaggcctgct cgacgtcggt gccaacggca tgaacgagct
     3781 cggctgggac ccggagtggg cggcatccgt atccgtagcg gaagcacggg agtgcgacga
     3841 cgctcagact ctgctgagca acgccgccaa cgactcccta cccatgaact gcatcaattg
     3901 gtacgaagcc tatgccttct gcatctggga tggaggcttc ttgcccagcg aggccgagtg
     3961 gaactacgcc gcgagcggtg gcgaagaaca gcgcgtctac ccgtggtcca agccggcgaa
     4021 ttcaacgaac atcgcctgct ccttcgcgaa ctacacccag gagggttata cggtaggttg
     4081 caccacgccc ccgaagggct ccccaacgtg gatgacggga tcgcccaatc gcgtcggttc
     4141 ggagtccccc tttggagacg ggaagtacgg tcacgcggga ttggcgggaa acgtggcgga
     4201 gtgggtgctc gattggtcct ggacttacgg cgcctgtacc aactgtgctc ggacgaccgc
     4261 ggccgccaac gacattacgc gcctgcgagt cgcccgcggt ggagacgcgc aagccccgca
     4321 aagctcgctt caaaatggcg cgcgaggcgt caccccgccc agctcccgga tctattccac
     4381 gggcgtgcgc tgcgcgcgga cgctctagcg cgttgctcac gaacgacgaa gccgcgatcg
     4441 actacttcga gtcaggagct cgagccaggc tcgcgagcgc ggcgaagctg accgggcgta
     4501 acggtgggcg cggggtgatg ccgtgccccg gttcgccacc gagctccagc aagcgccgcg
     4561 cgcaggggac caggcacgat ttgccctggc agtagcccgt tccaaacccg gtatagcgct
     4621 tcagggattc gatgtcggtg tggccaaggc tcaccgcgtg gtcgacctct tccacggtga
     4681 cgtcttcaca acgacacaga atccgtttca tctcagttcc gcgacagatc cgaacatacg
     4741 aaagccgaat cccctgccgg cttcatgcca cggaactcca cgtcgcacgt gtacgggcgc
     4801 tgcaagctct ctgcgagttc gacgaccagc gcggaagccc agtcacgcgc cccatcgaag
     4861 cgagcccgga tcacaccctt cgggtcgatg aaccacgtct ctgggtaaag cttcgttccg
     4921 taaagatcgg tcaccacctt ggcgtccgga tcgatcaacg tgacgaacgg tgcgtcagcc
     4981 cccaacacgg agcgcaaggt gtctctcgcg tcctccgcgc tgtcatccgt agtcaccgtc
     5041 agaagcacga tgtctttgcg ctcgcgcaac accttcgcaa gacccgcaat ggacggcatc
     5101 tcctccaagc aaggagggca agacttcgac cagaagttca agattaccgt cttgccccta
     5161 aatgagctga ggcggacctt gccacctcct acttggggga gctcgaagtc tggcgcaagc
     5221 cg
//
LOCUS       DASTKU010000771        14547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1267997, whole genome shotgun sequence.
ACCESSION   DASTKU010000771 DASTKU010000000
VERSION     DASTKU010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14547
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267997"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1722)
                     /locus_tag="VFQ61_26870"
     CDS             complement(<1..1722)
                     /locus_tag="VFQ61_26870"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9958159.1"
                     /translation="MPLLTLNDYGAHSLSVRAKALVFEDPQSRALLERLEQVAPSEAT
                     VLITGETGTGKEIVARYIHQLSRRSDKAFVAVNCGALTESLIESELFGHEKGAFTGAL
                     GSKAGWFEAANGGTLFLDEIGDLPISMQVKLLRVLQEGEVVRVGARQSTVVDVRLVAA
                     TNVSLGDAVAAGRFREDLFYRLHVASVTLPPLRERPADILPLARYFVRLYEQRLGVGG
                     IVFDSSAVARLLQHSWPGNIRELENVIHHALLVCKHSEISAEDLRLAALPARFSPAKA
                     PPASVGPASPTSSASAASTSVSGHELLSAALVQLFDVGLPDLYDQIERTVMRTAFEYC
                     DRNQLQTARLLGISRNVVRARLIEHGEIAGSVRPSPNQTPKPAATPLGSRASTRGEPR
                     RPLKIGFQKFGLLPLLKSEGALAADFEAHGFELDWIEYPGGIQIIEAFKSGDLAFGSV
                     GEGPPVFAHAESIPFVYLAAEEPAPEDEALVVLPHSSIRSVRELVGRRVALNRGANVH
                     YLLLRALEEAGIRYEDLEVVYLTPERARAALAAGEVDAWAIWNPLLNELVQAQSVRVL
                     RDAAGLAD"
     gene            complement(1870..2226)
                     /locus_tag="VFQ61_26875"
     CDS             complement(1870..2226)
                     /locus_tag="VFQ61_26875"
                     /inference="COORDINATES: protein motif:HMM:NF021593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HET9958160.1"
                     /translation="MHIAMVGGLERGEHHYASAAAQRGHSLEVHGGHLAGRGGDQLQR
                     MVERADVVIVVTDVNSHAAVLGARRFARSLGKPCLLVRRFGLARFRNLLGELDHFEER
                     GADQPDWSRLMASGWS"
     gene            complement(2380..3471)
                     /locus_tag="VFQ61_26880"
     CDS             complement(2380..3471)
                     /locus_tag="VFQ61_26880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958161.1"
                     /translation="MRNWLLTSIGTAALLHGACVPEFSNDLSQVRGARIVGIRSEPAE
                     AREGAMVTFEALWVAPLDAETPNIEYHFCLDRTPLTELGSVNPSCAAEPPPPGKLADS
                     SSNAAPLQSLGSGVRVDTAIPNDACRLFGPRRPEPKPGEPSGRPVDPDPTGGFYQPVV
                     AWIEPQHRALGSTRLDCGLTGAAQATQREFASRYRSNQNPELADLQIVHEDGTIVSSA
                     GRDRAVSEPARLGLAERVTLRALWAACPREPTCGDHICGEGEDLVNCAEDCTRPRGCT
                     GAESYAFYDREARTLVRRREGITVSWYTTAGHFSSERTGRSEQDVDESETTVEYRAPD
                     RPGEVGLWAVIHDDRGGTGIQFLKIDVGL"
     gene            complement(3471..6140)
                     /locus_tag="VFQ61_26885"
     CDS             complement(3471..6140)
                     /locus_tag="VFQ61_26885"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9958162.1"
                     /translation="MVSVRSLLAGVLLSSSACLSSGRVWAQSSLGPAARSTAPKPVLT
                     RPPEILEFVDAEYPESEKAQGRAASVVLELAISPLGTVDAARVVESAGIAFDEAALAA
                     ARRFRFTPAEIDGRPAPVRIRYRYDFVLREQVPSFGKLTGVVRDKKKGTPLAGVTVQL
                     SGQPSVTTDAAGRFEYAEVEPGTYTLSFSRADLRGLQTSESIEAGRSLEAIYEVEQAP
                     SEPEAAEDSDDLEIVVVAPRLTKQVVSTRVGAEEAQRVAGTQGDVLKVVENLPGVTRA
                     TAGSGQLVVWGAAPGDTRVIVDGVDLPALYHYGGVRSVMHGDLVRSVELLPGGYGAAF
                     GRGTGGLVRVESQELALERLRGSAQLDVLDASAALRGPLNNRVQASASARKSHLGWLL
                     NRVTSKDLGDFFPIPQYADGSARVRLAIGDGEWLELSGLASWDRVTRSIASEDPLDRR
                     SQTRQLNFQRVFLRYQREPGDGTRVVILPWVGADQRDLVAQFSSVASALHSRSSIVGL
                     RASYEGRIDSTLSGTLGFDLQLVSTEVRRTGSIGTPAREGDAFVFGQAPPDRSNSDTW
                     RVLQGSAAPYVEADWAPWGERFHIVPGLRLEPTFQSVPRRQPQEGSHPGVGVYRSDVV
                     LEPRISLRYSPTTRASFRAAVGRYHQAATPEDLSAVFGNPLLGPSDATHWLAGVNVRV
                     ASGLSLETTAFYTRTEQIAVRNPIDSPLVAQALLDNGQGRAFGAQFLLRREATNGFMG
                     WAAYTILRSERRDDPSGDWRLFDYDQTHVFTGVASYELGAGFDVGLRLRYATGYPRTP
                     VLGAYEDTRRGIFQPIVGARNGSRIPDFIQLDARLARRQRLAGGDLELYADVQNVTDR
                     DNPEEIAYSPDFSARRYILGLPILPVVGARWSF"
     gene            complement(6167..6895)
                     /locus_tag="VFQ61_26890"
     CDS             complement(6167..6895)
                     /locus_tag="VFQ61_26890"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HET9958163.1"
                     /translation="MSSRSRTSGHKWLLGFSLAAHGALGLAISVAEIKKAPAATAIEL
                     ANIPHKKKAVPAPHSESAVPKAPEPSRSPARPRAAKPAPPASPPPTAPQVVTPAAALD
                     ALPDFGLALSGGSASGLAVSANRSPPPQTATSSAPARKTINTPPLLDGCDEPATKPKP
                     RSVPNPGYTEAARVAGVEGKVRVEVTIDETGRVVSVRVLAGLGYGLDEAALAAARNAE
                     FEPAQRCGKPVRATFNIGMRFTAG"
     gene            complement(6973..7380)
                     /locus_tag="VFQ61_26895"
     CDS             complement(6973..7380)
                     /locus_tag="VFQ61_26895"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HET9958164.1"
                     /translation="MAAALGRGARGPIVGINVTPLVDVVLVLLIIMMVSATYIVSQSL
                     NVELPKTASSDDSVNRTYVVTITKDGKYLFNDQPVDPAQLDSRLRGAQAETKEVTLVV
                     SADQAALHGQVVGVIDRAKVVGISKFAINVELK"
     gene            complement(7427..8149)
                     /locus_tag="VFQ61_26900"
     CDS             complement(7427..8149)
                     /locus_tag="VFQ61_26900"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HET9958165.1"
                     /translation="MLIERLMKVALLGSAWVLYLLIGLSVLSFSVMLERWWFLRKRRD
                     DLVTLRRRVVERLVEQDWAGADDVLGKSQSLEARVVRKALNFRGGGSHAMTDGMESEL
                     LLVRKELERGLTLLGTLGNNAPFVGLFGTVLGVIIAFHALGAAGKNTSQMGGVMAGIA
                     EALVATGVGLFVALPAVVAYNVIQKRIADIESDAQSLTKLLTAYVKTQGHKHGFKASA
                     VSSTQNGVGAGELEDLEAPALS"
     gene            complement(8246..9235)
                     /locus_tag="VFQ61_26905"
     CDS             complement(8246..9235)
                     /locus_tag="VFQ61_26905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958166.1"
                     /translation="MNGVLRWSTWWVTLASACLACGGEETLSGIDRPFRVRSAQFEAE
                     NIPGLPPLSVEELQAGVAPTPPNVTSFSLNASLVPGTPNVALQGRATTDVTAVALALP
                     DAGPGYWVVPVGPPDPSFEGEYAWSANFDIGHSLAPGLHELVMAGIDGAGHSGTQATT
                     RLCVASPIPDNLNACAPKRKPPETVISLSWDAPVDLDLQVLTPGGKWVDAKHPTDAAL
                     DSTGKIGNSSRVTGVLQEDGGAGCLGRGRLSEHLVFSERPPAGVYSLHANLYSACTQA
                     SVRFELSIWSAAEGEEPGTFRQKKTYSVVGIQLASQANQGTQRGQFLTELVVE"
     gene            complement(9232..10455)
                     /locus_tag="VFQ61_26910"
     CDS             complement(9232..10455)
                     /locus_tag="VFQ61_26910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958167.1"
                     /translation="MRSRPGPSPSFAPGREERRAPSWQDGFSVSGYVQAQYEQNQLSE
                     DQLDASAAPLNSDRFVLRRGRVRLDKRWQYAQAVLELDANTVRGLAVGIRRAEASLVY
                     RRGNADLPVLALTAGILDIPFGYELAESPRTRPFFERSTTSLALFPSEADLGVKLWGA
                     FGFARYAVAITNGEPLDARGFPRDPNRAKDVSGRLGIDLQVQRRLALAAGTSFSFGKG
                     FHAGQVGTKGGVTWRDDNENGAVDAGELIGVPASAPEPSKNFERFALGLDLRAVLSTP
                     LGSSQLAFEGYLASNQDRALYPADPVQTGVDLRESGFSISFVQDVYRYGLIGLRYSLY
                     DPNADWLDQRQGNFQPRSAAVRSVSPVIGLNLADYARLLFQYDFVRDRLARDPRGVPA
                     DANNNWFGVRLQVGL"
     gene            complement(10878..11417)
                     /locus_tag="VFQ61_26915"
     CDS             complement(10878..11417)
                     /locus_tag="VFQ61_26915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958168.1"
                     /translation="MNWLSKSYRALSGLGLIAVLGAAQACGDDDDNATPSIPRGGSSS
                     RGGATSSGGADAAGESSTDGNSGGRTSGGRSSSNGGSSPQTSAGESNAGGNSEGGAST
                     AGGHANVEGGSGPVGAGGEGAGPSACTNPTGPGGCYACDPDGSDDLQFLNRCSDSDCS
                     PFDNSQRIPGFSGTLPPLN"
     gene            complement(11417..12637)
                     /locus_tag="VFQ61_26920"
     CDS             complement(11417..12637)
                     /locus_tag="VFQ61_26920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958169.1"
                     /translation="MVFDTATRVLLSIGLGAATIAAVSSAQAALCSELNLPNPIYGAG
                     GSAITAELAKVAEELAKFEPPITILWADPGACTGYQYFVSGDFGGTGTRPLKYWSATG
                     QALTCDLPIGEVVAPQFAHMGNTAEFCAGQSLPADVRDFAGPIQTVNLITDKDSSQKS
                     ISAEALYYIWGLGPSASGVSPWTDETALWGRNTSSFVHLFLAAAVGVPSNGFKLLSDH
                     VVSNQSQVISGIVASGNTNADASLGYVSSSAADAARASVKTLAYQHTGQLCGYWPDSS
                     EDALDKINVRTGKYHLWTPGHFFAKVDQRGAIVEPNVRKLIACFTDQEVSPGDVITRR
                     IIEAGDVPQCAMRVKREGVLGALSSFAPPAPCGCYFEAVATQAVPSSCTACEDDNGCD
                     GETPKCHHGFCEAY"
     gene            13341..14333
                     /gene="cysK"
                     /locus_tag="VFQ61_26925"
     CDS             13341..14333
                     /gene="cysK"
                     /locus_tag="VFQ61_26925"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET9958170.1"
                     /translation="MSERTTHRWFADNALTIGRTPLVRLNRITDGAPATVLAKIEGRN
                     PAYSVKCRLGAAMVWDAEQSGRLKPGRELIEPTSGNTGIALAFVAAARGYPLTLTMPE
                     TMSLERRKLLAAYGAKLVLTEGARGMAGAIAKAEEIAASAPDRYVLLQQFKNPANPRI
                     HETTTGPEIWDDTEGNVDIFVSGVGTGGTITGVSRYFKQNRGKALRSVAVEPSASPVL
                     AQKRAGQPLKPAPHKIQGIGAGFIPDVLDLSLVDEVEAVTNEEAIAYARRLAREEGIL
                     AGISCGAAAAVAVRLAKQRENEGKTIVVILPDSGERYLSTALFEGVFDAQGLSV"
     gene            14330..>14547
                     /locus_tag="VFQ61_26930"
     CDS             14330..>14547
                     /locus_tag="VFQ61_26930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HET9958171.1"
                     /translation="MSSPALRVVADTHWDLGRVVNELRRSRHADKTQPDGESRLVTHQ
                     LPSRAALLRVASGLTAALFPSHFGPPDLT"
BASE COUNT         2853 a         4712 c         4390 g         2592 t
ORIGIN      
        1 gtcggccaaa cctgcggcgt cacgcagtac ccgcaccgat tgagcttgca cgagctcgtt
       61 cagcagaggg ttccagattg cccaggcgtc gacttcaccc gcggccaacg ccgctcgcgc
      121 ccgctccgga gtgagataaa cgacttccaa gtcctcgtat cgaatcccgg cctcctcgag
      181 cgccctcagc agtaaatagt ggacgttggc ccctcgattc agagccacgc ggcgccctac
      241 caattcgcga acgctgcgga ttgacgagtg aggaagaacc accagggcct catcctcagg
      301 tgcgggctct tcggcagcca gatacacgaa cggtatgctc tcggcatgag cgaacacggg
      361 agggccttca ccaacactgc cgaacgcgag atcgcccgac ttgaacgcct cgatgatttg
      421 gattccaccc gggtactcga tccaatcgag ttcgaaaccg tgcgcctcga agtctgcagc
      481 cagagctcct tccgacttca agagcggcag cagcccgaac ttttgaaacc caatcttgag
      541 cgggcgacgt ggctccccgc gtgtggacgc ccgggagccc aggggagtgg ccgctggttt
      601 gggagtttga ttcggggacg gccgcaccga gcccgcgatc tccccatgtt cgatcaacct
      661 cgcgcgcacg acgttgcgac taatgcccag cagcctggcc gtctgcagct ggttacgatc
      721 gcagtattcg aaggccgttc gcatcacggt gcgttcgatt tgatcgtaca agtcgggcag
      781 gcccacatcg aagagctgca ccaatgccgc gctcaagagt tcgtgacccg aaaccgatgt
      841 ggacgcggcc gaagccgagc tcgtaggcga agcaggccca acgctcgcag gaggcgcttt
      901 ggcagggctg aaccgggccg gcaacgccgc tagccgcaag tcctccgcgc tgatctccga
      961 gtgcttgcag accagcagag cgtgatgaat cacgttctcg agctctcgaa tgttgcccgg
     1021 ccaactgtgc tgtaggagcc gcgccaccgc actcgagtcg aacacgattc cacccacgcc
     1081 cagacgttgc tcgtaaagcc gcacgaaata tcgggcgagc ggcaagatgt cggccggcct
     1141 ttcgcgcagc ggaggcagcg ttaccgaggc tacgtggagc cgataaaata gatcctcgcg
     1201 gaaacggccc gccgccaccg catcgcctag agacacgttg gtcgcggcca cgagacgcac
     1261 gtccaccacc gttgactgtc gcgccccaac acgcaccacc tccccttcct gcagaacccg
     1321 tagcaacttg acttgcatcg agatcggcag atcgccaatc tcgtcgagaa acagagtacc
     1381 gccattggcg gcctcgaacc aaccggcctt gctccccagc gcccccgtga acgccccctt
     1441 ctcatgtcca aagagctcgc tctcgatcag cgattcggtc aaggcaccgc agttgaccgc
     1501 gacgaaggcc ttgtcgctcc ttcggctcag ctgatggatg taccgtgcga ctatttcctt
     1561 accggtgccc gtctcgcccg taatgagcac cgttgcctca ctaggagcga cctgctccag
     1621 gcgctcgagc aatgcgcggg attgaggatc ctcgaaaacc agcgctttcg cgcggacaga
     1681 caaggagtga gcgccgtagt cgttgagagt gagtaaaggc attttggtgt gatggggttc
     1741 caaaaacagc tcacgtcggc tggatgcgcc aacgtacagc tcgtgacacg gtggccgtcc
     1801 ctcatcgccc acactctctg ctgaaacgtc gtcaagctct cctccgagtc gcgtctcgag
     1861 cgctccggct caggaccaac cagaagccat cagccgcgac cagtccggtt ggtccgcgcc
     1921 gcgttcctcg aagtggtcca gctccccgag caggttccgg aagcgcgcca acccaaagcg
     1981 tcgcacgagc aagcagggct tgcccaagga gcgcgcgaat cgccgggccc cgaggactgc
     2041 ggcgtgactg ttcacgtctg ttacgacgat cacgacgtct gctctttcga ccatacgctg
     2101 aagctggtcc cctccgcgtc ccgcgaggtg ccccccgtgg acttccaggg aatggccacg
     2161 ctgcgcggct gccgaagcat agtgatgctc gccacgctcc aaaccgccaa ccatcgcgat
     2221 gtgcataagt cctcgttttc cgcggagaag ttattccgta ttctccatcg gattactagg
     2281 ttatggtccg aagcctgtca agccgcggca cccagacccg ccacgccggc gtagagaaaa
     2341 tgtgccgctg ttcgcgggct tagggactct gatcttattt tatagaccta cgtctatttt
     2401 aaggaactga attccggttc cacctcggtc gtcgtgaatc actgcccaga gcccgacctc
     2461 gccaggacgg tctggcgctc ggtattcgac cgtagtctcg ctttcgtcca cgtcctgctc
     2521 cgagcggccc gtgcgctcgc tactgaagtg gccggcggtc gtgtaccagg ataccgtgat
     2581 gccctcccgg cgccggacga gtgttcgagc ttcccggtcg taaaaagcgt agctttcggc
     2641 accggtgcag ccacggggac gggtgcagtc ctctgcacaa ttgaccaagt cctcgccttc
     2701 gccacagatg tgatctccgc aagtcggttc acgcgggcac gccgcccaga gagcgcgcaa
     2761 ggtaactcgc tcggccaacc ccagccgtgc tggctcagaa acggctcggt ctcgacccgc
     2821 agagctgacg atcgttccgt cctcgtggac aatctgcaaa tccgccagct ccgggttctg
     2881 attgctccgg tagcggcttg cgaattcgcg ttgagtcgct tgcgcagcgc cggtcaaccc
     2941 gcaatccagc cgcgtactgc cgagcgcccg atgttgaggc tcgatccagg cgaccacggg
     3001 ctgataaaag ccaccggtgg gatccggatc caccggtcta ccgctcggct cacccggttt
     3061 aggctctggg cgacgcggac caaataggcg acaagcgtcg ttcggtatgg cggtatcgac
     3121 ccgcaccccg gaaccgagac tctgcaaggg ggcggcattc gagctggagt cggccagctt
     3181 ccccggcggc gggggttcag ccgcgcaact cgggttcacc gagccgagct cggtcaaagg
     3241 cgtgcggtcc aagcagaagt gatattcgat attcggagtc tcggcgtcca acggagcgac
     3301 ccaaagcgcc tcgaaggtga ccatcgcgcc ctcgcgagct tcggcgggct cactccgaat
     3361 gcctacgatg cgcgccccac gcacctgcga cagatcgttc gagaactccg gaacgcaggc
     3421 cccatgcaga agtgcggccg tcccgatcga ggtcagtagc caattacgca ctagaaactc
     3481 catcgcgcgc caaccacggg cagaattggt agccccagaa tatagcgtcg agcgctgaaa
     3541 tcagggctat aagcaatctc ctctgggttg tcacgatcag ttacattttg tacatcggcg
     3601 tagagctcga ggtcaccacc ggcaaggcgt tgccgccggg cgagccgcgc gtcgagctga
     3661 atgaagtcgg gaatgcgcga gccgttgcgc gcccccacga tcggctgaaa tatcccgcgc
     3721 cgcgtatcct cgtaggcgcc cagcacgggg gtgcgaggat agccggtggc gtaacgcaaa
     3781 cgcaggccga catcgaaccc ggcgccaagc tcataggaag ccaccccggt gaagacgtgg
     3841 gtttgatcgt agtcgaacag tcgccaatca ccgctcgggt cgtcccgacg ctcactacgc
     3901 aaaatggtat atgcggccca ccccatgaag ccattcgtcg cctctcggcg aagcaggaac
     3961 tgcgccccga aagctcggcc ctgcccattg tcgagcagcg cctgagccac gagcggagag
     4021 tcgatggggt ttcgcaccgc gatctgctcc gtacgggtat agaacgccgt cgtttcgagc
     4081 gataggccgg aggcgacgcg aacgttcact ccggctaacc agtgcgtcgc atcggagggc
     4141 cccagcagcg gattgccaaa aaccgccgac aagtcctcgg gagttgcggc ttgatgatag
     4201 cgacctaccg ccgcccggaa gctcgcgcgg gtagtgggcg agtaacggag cgagatccgc
     4261 ggttcgagca ccacgtcaga acggtacaca ccaacgcccg ggtgtgatcc ttcctgcggt
     4321 tggcgacgcg ggacgctttg aaaggtaggc tccaggcgca gccctggcac gatgtggaac
     4381 cgctcgcccc aaggcgccca atccgcctcg acgtacggag ccgcactgcc ctggagtacg
     4441 cgccaggtat cgctgttact tcgatcgggt ggcgcctggc cgaacacgaa ggcgtcgccc
     4501 tctcgcgctg gcgtgccaat cgagccggtg cgtcgcactt cggtcgaaac gagctggaga
     4561 tcgaaaccga gcgtgccgct cagggtggag tcgatgcgtc cttcgtagct cgcgcgtaag
     4621 ccgacgatgc tcgatcggct atgtaaggcg ctggctacgc tggagaactg cgcgaccaag
     4681 tcgcgttgat ctgcgcccac ccaaggcaga atcacgacgc gcgtgccgtc gcctggttca
     4741 cgttggtagc gtaggaacac gcgttgaaaa ttcagctggc gcgtctgtga tctacggtcg
     4801 agcgggtcct cacttgcgat gctgcgggtg acgcggtccc aagaggcgag gccgctcaac
     4861 tccagccact ctccgtctcc gatagccaag cgcacccgcg ccgaaccatc tgcgtattga
     4921 gggatcggaa agaagtcccc taggtctttg gacgtgaccc gattcaagag ccaccccaag
     4981 tggctcttgc gtgcgctcgc agacgcctga accctgttgt tcagggggcc gcgcagcgcc
     5041 gcagatgcgt cgagcacatc cagctgtgca ctgccgcgca atcgttcgag cgcgagctct
     5101 tggctctcga cccgcacgag acctccggtg cctcgaccaa aagccgcgcc gtatccgccg
     5161 ggcaacagtt cgactgaacg caccaaatct ccgtgcatga ctgagcgcac gccgccgtag
     5221 tggtacaacg ccggcaaatc cacgccatct acgatgacgc gcgtgtcccc aggagccgct
     5281 ccccacacca cgagctgacc ggaccccgca gtcgcgcgag tcacgcccgg cagattctcg
     5341 acgaccttca ggacgtctcc ctgggtgccg gcaacgcgct gggcttcttc ggcaccgaca
     5401 cgagtggaca ccacttgttt ggtcaacctg ggagcaacta cgactatttc gagatcatcc
     5461 gagtcttcgg cggcttcagg ctccgacggg gcctgctcca cttcgtaaat cgcctcgagc
     5521 gatcgccctg cctcgatgct ttcggaggtc tgtaaaccgc gtaagtccgc gcgggaaaag
     5581 ctcaatgtat acgtacctgg ctcgacctcg gcgtattcga accgtcccgc tgcatcggta
     5641 gtaaccgatg gttgcccaga gagctgcacg gtcacgcccg ccagcggggt tcccttcttc
     5701 ttatcccgaa ccacgccggt caatttaccg aatgagggta cctgctcgcg caacacgaaa
     5761 tcatatcggt atcgaatccg gacgggggct ggcctgccgt caatctcggc gggtgtgaag
     5821 cgaaacctgc gcgcggcagc cagagccgct tcgtcaaacg cgatcccagc ggactcgacg
     5881 actcgcgccg catcgaccgt gccgagcggc gaaattgcga gctccaatac gaccgaagcg
     5941 gcgcggccct gagccttctc actctctggg tactcggcgt cgacgaactc caggatttct
     6001 ggcggtcgcg tgaggacggg cttgggagca gtggagcgcg cagcgggccc aaggctactt
     6061 tgcgcccaga cgcgacctga actcaagcag gccgaactcg agagcaagac cccagccaag
     6121 agactacgaa ctgacaccat ttgatacttc gcgaggccca cgaggctcaa cccgccgtga
     6181 agcgcattcc gatattgaaa gtggcacgaa cgggctttcc gcaacgctgc gcgggctcga
     6241 actcggcatt gcgtgcggcc gccaaggcgg cttcgtcgag cccatagcct aggccggcca
     6301 ggactcgcac agagaccact cgacccgttt cgtctatcgt gacttccacg cgcaccttgc
     6361 cctccacacc cgcgacgcgc gcagcttctg tgtaccctgg gttcgggacg gaacggggtt
     6421 tgggcttggt ggcaggctca tcgcaaccgt cgagcaaagg cggggtgttg atggttttgc
     6481 gggccggagc agagctcgtt gcagtctgcg gcggtggtga gcgattggca gaaacggcaa
     6541 gccccgatgc cgaaccgcca ctcagagcca acccgaaatc cggtaaggca tctagcgcgg
     6601 ccgcgggagt caccacctga ggggccgttg gtggagggct cgctggaggt gcaggcttgg
     6661 ccgcccgggg ccgtgctggg ctccgcgacg gctctggcgc tttgggaacg gcagattccg
     6721 agtgcggcgc gggaacagcc ttctttttat gagggatgtt cgccaattcg atcgcggtcg
     6781 ccgcgggagc cttcttgatc tccgccacgg aaatcgcgag cccgagcgcg ccatgcgcgg
     6841 cgaggctaaa accgagcagc catttgtgcc ccgaggtccg cgagcgagag ctcacgagtt
     6901 cggaatgcag aggtgagccc gcaggctgta ccgtagcgcg gggcgcctcg ggcgaactac
     6961 gatcgcgcgt gttcacttga gctcgacgtt gatggcgaac ttgctgatgc ccactacctt
     7021 ggcgcgatcg atgacgccaa cgacctgccc gtggagcgcc gcctggtctg ccgaaaccac
     7081 gagcgtgacc tccttcgtct ctgcttgcgc gccgcgcagg cgggagtcga gctgagcggg
     7141 gtctaccggc tgatcgttga acaggtactt cccgtccttg gtgatggtca ccacgtaggt
     7201 acggttcacg ctgtcgtccg aggacgcggt cttgggcaat tcgacgttca agctctgcga
     7261 cacgatatac gtcgccgaaa ccatcatgat gatgagtaac accaacacga cgtccaccaa
     7321 gggagtaaca ttgattccga caattggccc gcgcgcgcca cgccctaatg cagcagccat
     7381 gacaacctct ccaccctgaa gaactgaata gaaacccgca tgccgttcaa gacagcgcgg
     7441 gcgcttcgag gtcttccagt tctccggcgc ccacgccgtt ctgcgtcgaa ctcaccgcgc
     7501 tcgccttgaa gccatgcttg tgaccctgag tcttcacgta tgccgtaagt agcttggtga
     7561 gcgactgcgc gtccgactcg atatccgcga tgcgtttctg aatgacgttg taagccacca
     7621 ccgccggcag ggccacgaac aggccgactc cggtggcgac gagtgcctcg gcgatacccg
     7681 ccatcacccc tcccatctgc gaggtgttct tgccggccgc accgagcgca tggaaggcga
     7741 tgatcacgcc cagcaccgta ccgaacaagc ccacgaacgg cgcgttgttg cccaaggtac
     7801 cgagcagggt cagaccgcgc tcgagttcct tgcgcaccag cagcagttca gattccatgc
     7861 catcagtcat tgcgtgtgaa cctccacctc gaaagttcag tgctttacgc actacccgcg
     7921 cttccaacga ctgactcttg ccgagcacgt catctgcacc cgcccagtcc tgctcgacca
     7981 atcgttcaac cacgcggcgc cgcaacgtca cgagatcgtc ccggcgctta cgcaggaacc
     8041 accagcgctc gagcatgacc gagaacgaga gcacgctcaa gccaatcaag aggtacagca
     8101 cccaggcact gccaagcagc gccactttca tcaaacgttc aatcaacatg ctcgttacct
     8161 tgcgcgaaag gaggtcagtt gaggggatcg gacgagcgga cggattcgaa gtaggcacgt
     8221 tcgaacgctc acgggagagc gcggctcatt ccacgacgag ttcggtaagg aactgcccac
     8281 gttgcgtgcc ttggttcgcc tgcgaggcga gctgaatccc aacgacggag tacgtcttct
     8341 tctgcctaaa cgtgccaggt tcctcaccct cggcggcgct ccaaatcgag agttcgaaac
     8401 gcacgctcgc ctgcgtgcac gccgagtaga gattggcatg cagagagtac actccggcgg
     8461 gaggtcgctc cgagaagacc aagtgctcgc tcaatcgccc gcgtcccagg catcccgcgc
     8521 cgccgtcctc ctgcaggaca cccgtcacgc ggctcgaatt gccaatctta ccagtggagt
     8581 ctagggcggc gtcggtggga tgtttggcat ccacccactt gccgccggga gtcagcacct
     8641 gcaagtcgag atcgaccggc gcgtcccaac tcaaactgat gacggtctcg gggggctttc
     8701 gtttgggtgc gcacgcgttg agattgtcgg ggatcggtga agccacgcac aggcgcgtcg
     8761 tcgcttgcgt tcccgagtgg ccagcaccat cgatcccggc catgaccagc tcgtgcaacc
     8821 ccggtgcgag cgagtgtccg atatcgaaat tggcagacca ggcatattcc ccctcgaaag
     8881 aaggatcggg aggccccacc ggaacaaccc aataaccggg ccccgcatcg ggcaaagcca
     8941 gagcgacggc cgtcacgtcg gtcgtggcgc gcccctgcag cgcgacattc ggagttccgg
     9001 ggacgaggct cgcattcaga gaaaagctgg tgacgttggg aggcgtggga gcgaccccag
     9061 cttgaagctc ctcgacgctg agcggaggca atcccgggat gttctcggct tcgaattgcg
     9121 ccgaacgcac gcgaaaaggt cgatcgatcc ccgataacgt ctcttctccg ccgcaagcga
     9181 ggcacgccga cgcgagcgta acccaccagg tggaccaccg aagcactccg ttcatagccc
     9241 cacctgcagg cgcactccga accagttatt gttggcatcg gccggcacgc cgcgcggatc
     9301 ccgggccagc cgatcgcgca cgaaatcgta ttgaaacaac agccgcgcgt agtccgccag
     9361 gttgagtcca atcacgggcg agacgctgcg gaccgccgcg gagcgcggct ggaagttccc
     9421 ttgccgctga tcgagccaat ctgcgttggg atcatagagc gagtaacgca gcccaatcag
     9481 tccatatcgg tagacatcct gcacgaacga gatcgagaaa cccgattcac gcagatccac
     9541 cccggtttgc accggatccg cagggtagag ggcgcgatcc tgattcgacg ccaagtaccc
     9601 ctcgaaagcg agttggctcg aacccagcgg tgtggacaag actgctcgca ggtcgagacc
     9661 caacgcaaaa cgctcgaagt tcttggaagg ctctggtgca gaggcgggca ccccgatcaa
     9721 ctcgcccgcg tccaccgcac cattttcgtt gtcgtcgcgc cacgtcacac cacccttggt
     9781 gccgacttgg cctgcgtgaa agcctttacc gaaggagaac gacgttccgg ccgcgagggc
     9841 gagccgccgc tggacttgca ggtcaattcc taggcgaccg ctcacgtctt tggcgcggtt
     9901 cgggtcacgc ggaaagccgc gggcgtcgag cggttcgccg ttcgtgatgg ctaccgcata
     9961 acgcgcaaac ccaaaggcgc cccacagctt cactcccaaa tcggcctcgc tcggaaacaa
    10021 ggccaacgac gtggtgctgc gctcgaagaa aggccgcgtg cggggggact cggcgagctc
    10081 gtatccaaag gggatatcga gtatccccgc cgtgagggcc aataccggaa gatccgcatt
    10141 tccccgtcga tacaccaaag acgcctccgc acgacgaatg cccaccgcga ggcctcgcac
    10201 cgtattggcg tctagctcca agaccgcttg ggcatactgc caccgcttgt ccaagcgaac
    10261 acggccccgc cgcaagacga agcggtccga attgagcggg gccgcgcttg catccaactg
    10321 atcttccgag agctggttct gctcgtattg cgcctgcacg taacccgaga cactaaaacc
    10381 atcttgccag gagggagcgc gtcgctcctc gcggcccgga gcgaagctgg gcgacggtcc
    10441 aggccgagag cgcactccgg aggactcgtc ctccggctgc tgaacatccg gtttcgactt
    10501 cacagccgtc ggctgcgact gcaccgccgg ttcatcaggc gccgacggta ctgcagacgt
    10561 ggttggcgac acggcgggtg atggctcggg ggctgggttc ccagtcggcg tttccgcggg
    10621 agaaagcggt acagaggagg ttgaggacgg aacctcgaac tcctcctcgt catccttcgc
    10681 gcggggagcg ccctcgagcg gagcgccctg cgctagtgca gtcggggaga cactgacggc
    10741 gagggcaaaa ccccaagccc aaacgagctg tgaagacaag agtgcgtcca gttttcgacg
    10801 gtagctagtg ttgaaaattc tgacgggctg cccagcagtt cgcaggagcg cgcgctcagg
    10861 cgctcggatc gtggggctca gttgagtgga ggaagagtgc cgctgaatcc tgggatgcgt
    10921 tggctgttgt cgaatgggga acaatccgaa tcgctgcagc ggttcaagaa ctgcagatca
    10981 tcgctgccat ccggatcgca tgcataacac ccgccgggcc ccgtgggatt cgtgcacgcc
    11041 gaagggcctg ccccttctcc gcctgctccg acgggaccgc tgccgccttc gacattggcg
    11101 tgaccgcccg cggtgctggc cccgccttcc gaatttcctc cggcattcga ttcgccggca
    11161 ctcgtttgcg gactggaacc gccattgctg ctcgatcgcc cgccgctcgt acggccacca
    11221 gagttgccat ccgtagagct ctctcccgcg gcatctgctc cgccgctgct cgttgcgccg
    11281 cctcgcgaac tggagcctcc ccgcgggatc gacggcgtcg cgttgtcatc gtcatcgccg
    11341 catgcttgcg ccgcacctaa cacagcgatc aatcccaagc cggacagagc tcgatacgat
    11401 ttggaaagcc agttcattag taagcctcgc aaaatccgtg gtggcacttg ggagtctcgc
    11461 cgtcgcagcc attatcgtcc tcgcaggccg tgcaggagct cgggacggct tgagtcgcca
    11521 ccgcttcgaa atagcagcca caaggagcgg gaggagcaaa ggagctcagc gcaccgagca
    11581 ccccttcacg cttcacccgc atcgcgcact gcggaacgtc accggcctcg atgatccgcc
    11641 gcgtgattac gtctccaggc gacacttcct gatccgtgaa acaagcaatt agcttgcgga
    11701 cgttcggctc cacgatggcg ccgcgctggt cgaccttggc gaagaagtgc cctggcgtcc
    11761 aaaggtgata cttacccgtg cgcacgttga tcttgtcgag tgcgtcttcg ctcgagtccg
    11821 gccagtaacc gcaaagttgc ccggtgtgtt ggtaagccaa tgtcttgacg ctggcccgcg
    11881 cggcgtctgc ggcagaactc gaaacgtagc cgagcgaggc gtcggcattg gtgtttcccg
    11941 acgcaacaat cccactaatg acctgggact gattggagac cacgtgatcg gacagcagct
    12001 tgaacccatt cgagggcacg cccaccgccg ccgctagaaa gaggtgtacg aacgagctgg
    12061 tattgcgacc ccacaaggcc gtctcgtccg tccacgggct caccccagac gcgctcgggc
    12121 cgagacccca gatgtaatac aatgcctcgg cgctgatcga cttctgactc gagtccttgt
    12181 ccgtaatgag attcacggtt tggattggcc ctgcaaagtc ccgcacgtca gcgggcaagc
    12241 tttgccccgc gcagaactcg gccgtattgc ccatgtgagc gaattgcggc gcgacgactt
    12301 ctccaatcgg caagtcacac gtcagcgcct gccccgtcgc gctccagtac ttcagcgggc
    12361 gtgtgccggt gccaccgaaa tctcccgaca cgaagtattg gtatcctgta caagcgcccg
    12421 ggtcggccca cagtatcgtg atcggtggtt cgaacttggc tagctcttca gcgaccttcg
    12481 ccagttcagc ggtaattgcg ctaccgccag cgccgtagat gggattcggc aagttcagct
    12541 cggaacaaag cgccgcctga gctgagctca ccgccgcgat tgtcgccgcg cccaggccga
    12601 ttgaaagcaa gacccgagta gcagtatcga agaccattcc aattacctcg ttgagaacgc
    12661 actccggccg tttggggggc gcgcagaccg cctccccgcc tgcgtagtgc cctggggacc
    12721 agagcgcctc tcggtgcatg tgaacgtacg cgtggcgcgg acgaactagt ccccacgcat
    12781 actaagcgaa aaagctgatg ttccgagtac ttaccggacc gcgcaggagc tgaagaatta
    12841 ttttctatcg ggttagtaga ctatcgccac gccctcgtca agcactgggt tcaccatcca
    12901 gcaggaaggg gagtgactac cccctggagt gaaattcacg gcaggaccgc gatctgctgg
    12961 aggcttcggc acgacggagt acaggacata cgtctaggca ccgacctgac gacggcctcc
    13021 agctgcgtct gcaacattgc gcggtgccgt tggttcgtgt agcgtagcca gcgtgctcgg
    13081 tccgctttgc ttcggtttcc actgctcgga acgctgtacc gggctggcag gccagtgacg
    13141 gcttgccagc gtaatccctt tccgctctaa acagtgtttc tacgagcgac tgcgccttga
    13201 gtacttcatc ggcgagtcgg ctcgagcgag tccgtttcga tagccgcgtc aaagcaagtc
    13261 ggcttcaaaa gcgtcatcgg ctgtaacgct tgcaggcaac cgttggggcg tctgccgccg
    13321 gtgagaatag tggagttcat atgtccgagc gcacgacaca tcgttggttc gcagataacg
    13381 cactcaccat tgggcgcacg cctctcgtgc gcttgaatcg cattacggac ggagccccgg
    13441 ctacggtgct cgcgaagatt gaaggccgca accctgcata ctccgtcaaa tgccgtttgg
    13501 gcgcggccat ggtgtgggac gcggagcaga gcggtcgttt gaagcctggc cgcgagctga
    13561 tcgaaccaac cagcggcaac accggcatcg cgttggcatt cgtggctgcc gcccgaggtt
    13621 atccgttgac gctgacaatg cccgaaacga tgagtctcga acggcgcaag ctgcttgccg
    13681 cctacggggc caagctggtg ctgaccgagg gtgctcgggg catggcggga gcaatcgcga
    13741 aagcggaaga gatagcggcc tcggcgccgg atcgctacgt gctgctccag cagttcaaga
    13801 acccagccaa tccgcgcatt cacgaaacca ccaccgggcc agaaatctgg gacgataccg
    13861 agggcaacgt cgatatcttc gtctcgggcg tggggaccgg aggaaccatc accggtgtgt
    13921 ctcgttactt caagcagaac cgcgggaaag cgctccgcag cgtggccgtg gaacccagcg
    13981 ccagccccgt tctcgcccag aagcgtgctg gccaaccgct gaaacccgcg cctcacaaga
    14041 ttcagggcat aggcgctgga ttcataccgg atgtactcga tttgtctttg gtagatgagg
    14101 tcgaagccgt cacgaacgaa gaagccatcg cttacgcacg acgcctggct cgcgaggagg
    14161 ggattctggc cggcatttcg tgcggggccg ccgccgcggt tgcggttcgt ctcgcaaaac
    14221 agcgcgagaa tgaaggaaag accatcgtgg tgattctgcc ggattcgggg gaacgctacc
    14281 tgagtacggc gctgttcgag ggcgtcttcg atgcgcaggg gctatccgta tgagttcgcc
    14341 agctttgcgt gtcgtcgccg acacccattg ggatttgggc cgcgtcgtca atgagctgag
    14401 gcgatcgcgc cacgcggaca aaactcagcc ggatggcgag tcacggctcg tgacgcatca
    14461 gctgccatca cgagccgcac tgctccgtgt tgcgagcggg ctcaccgcgg cgctgtttcc
    14521 gtcccacttc ggtccacccg atttgac
//
LOCUS       DASTKU010000772         4318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_597499, whole genome shotgun sequence.
ACCESSION   DASTKU010000772 DASTKU010000000
VERSION     DASTKU010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597499"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VFQ61_26935"
     CDS             <1..1116
                     /locus_tag="VFQ61_26935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958172.1"
                     /translation="LLLALPFLKKTRKLWFLVAWIHVAIFAWYAVHHQDRYLQGIVPL
                     MAAVVAAILVSIFHGFGWLVRGACSLLVLAQIVWGGDVYFFQTHSMAKSALKKTLDLL
                     SAGFERKYEERLRVSGSLAALREYLPAGSRVLLHQRHPHLGLAAESVLDSFAWQFGLD
                     YGAQKTPEGVRRLLREQGITHVFFEPGKGNAMHPLANDLLFHEFASRFTTDRVRVGGA
                     TLARVPDQPSTRPFQGHVAVLNCESMPYPGLYETETLNVLEYGPKQDDYEAALAGADD
                     VHGALTLLPQADFAIVEPKCFPEGLPRALTSAFELFVKRQREPREIWRRTRTGAAVLE
                     ESSAPLQPSVTPESSRRPGRQPRSALEDNVDSSETDE"
     gene            1237..2283
                     /locus_tag="VFQ61_26940"
     CDS             1237..2283
                     /locus_tag="VFQ61_26940"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9958173.1"
                     /translation="MKLIVQIPCFNEAETLAIALAEIPREIPGVDTVELLVIDDGSSD
                     NTAEIARREGVHHVVGFHTNQGLAKAFMLGLHASLERGADIIVNTDADNQYNAADIPR
                     LIQPILEHRADMVIGARPISEIAHFSPIKKILQRFGSWVVRRVSGTDVADAPSGFRAI
                     SREAALSLNVFNGYTYTLETIIQAGQKGMSVVSVPIRVNGELRPSRLVRSIPNYVKRS
                     ILTMIRIFVVYQPMRFFIRLGLIPFTLGVLIGLRFLVYFFNGQGTGHVQSLILTAVLL
                     LIGIQTFLLAILSDLIGVNRRLLEELQRRERARLGFRAELGEKPSIPPLPDRRSSRPP
                     PIGLGTNREGTFNL"
     gene            2353..2434
                     /locus_tag="VFQ61_26945"
     tRNA            2353..2434
                     /locus_tag="VFQ61_26945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2387..2389,aa:Leu,seq:tag)
     gene            complement(2451..2750)
                     /locus_tag="VFQ61_26950"
     CDS             complement(2451..2750)
                     /locus_tag="VFQ61_26950"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET9958174.1"
                     /translation="MAVTWSKRAEADLREIFARIAVENRPAAERWIVRLIERVEDASE
                     APLLGRIVPELARAEIRETYLRTYRIVYRADETGVCVLTVFEGSRLLRRSDSDEP"
     gene            complement(2741..3469)
                     /locus_tag="VFQ61_26955"
     CDS             complement(2741..3469)
                     /locus_tag="VFQ61_26955"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HET9958175.1"
                     /translation="MPAGRVQAFRDALGDEFELDTSMLRDAILHARTANAFYLPYVTK
                     IDFFGRAYEAFDDSEFARRRSVIVRSTGESLVLKSPEDTVLRKLLWYRAGGEVSPRRP
                     GTARGIRGMALEADVPLAPIHCICGYTPLMDAAERRRAEQQARAAKTLTDKTPVTTAE
                     LSQRAAEIVKRVHETGEAVVVVQDGQPAAVLVDAEEFALLREHKRFVAAIEEGLADAD
                     AGRVLSTQDLKKSLETEFGPIAWQ"
     gene            complement(3475..3624)
                     /locus_tag="VFQ61_26960"
     CDS             complement(3475..3624)
                     /locus_tag="VFQ61_26960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958176.1"
                     /translation="MAADATFDVIDVALRVATSLEAVGANYFVGGSIASSLQGEPRAT
                     TTSTS"
     gene            complement(3614..3859)
                     /locus_tag="VFQ61_26965"
     CDS             complement(3614..3859)
                     /locus_tag="VFQ61_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958177.1"
                     /translation="MRDTSPEAEERYFELMRRQPPAARLAAAARLCSAVRALAEAGIR
                     DGHPGATEQEVRAHLAARMYGREVASRLFPNVNLDGR"
     gene            complement(4116..>4318)
                     /locus_tag="VFQ61_26970"
     CDS             complement(4116..>4318)
                     /locus_tag="VFQ61_26970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958178.1"
                     /translation="RVPVRYRWRHLDTARRRFKDDSKPVVKPTGGVELRTQRAVLLCR
                     ALAVQSREAEFGDAGVTRKRSP"
BASE COUNT          772 a         1436 c         1303 g          807 t
ORIGIN      
        1 ctcctgctgg cgctgccctt cctgaagaag acgcgcaagc tctggttttt ggtagcttgg
       61 attcacgtcg cgatcttcgc ctggtacgcc gttcaccacc aagaccgtta tctgcagggg
      121 atcgtgccgt tgatggccgc ggtcgtcgct gcgattctgg tcagcatctt ccacggcttt
      181 ggctggctcg tgcgcggcgc gtgtagcttg ctcgtcctgg cgcagatcgt gtggggtgga
      241 gacgtttact tcttccaaac ccactccatg gccaaatccg cgctaaagaa aaccctggat
      301 ctgttgagcg caggcttcga gcgcaagtac gaagagcggc tacgggtctc cggcagcctc
      361 gcggcgctgc gtgagtatct cccggcaggt tcccgcgtgt tgttgcacca acggcacccg
      421 catctggggc tcgccgcaga gtccgtcttg gattcgttcg cttggcagtt tgggctcgac
      481 tacggtgcgc aaaagacccc cgagggcgtg cgacgcttgc tgcgcgaaca gggcatcacc
      541 cacgtatttt tcgagccggg caagggcaac gcgatgcacc cgttggccaa cgacctcttg
      601 ttccacgagt tcgcgagccg cttcacgacg gatcgggtgc gggtcggggg ggcgacgctc
      661 gcgagagttc cggaccagcc gagcactcgg cccttccaag ggcatgtcgc ggtcttgaac
      721 tgtgaaagca tgccgtaccc cggcttgtac gagaccgaga ccctgaacgt gctcgaatac
      781 ggcccaaaac aagacgacta cgaggcggct cttgccggag ccgatgatgt tcacggagcg
      841 ctcacgctgc tcccgcaggc ggacttcgcg atcgtggagc cgaagtgctt cccggaaggt
      901 ttaccgcgag ccctcacgag tgcattcgaa ctgttcgtca agcggcagcg cgagccacgt
      961 gagatttggc gacgcacccg gaccggagca gctgtcctcg aggaaagctc ggcgcccttg
     1021 caaccctccg tgacaccgga aagttctcgc cgccccggcc ggcaacctcg gtcggccctc
     1081 gaagacaacg tcgattcatc tgaaactgac gaataggcgc ggtttgctta gttcgttttc
     1141 gcctcgatgc tcgacccggg tcacaatgcg cgcgcttacc ccgtcccgaa cctcctcggc
     1201 gccgcctcgc agcgcccccc gcatgccagc cagcccatga agctcatcgt ccaaatcccc
     1261 tgcttcaacg aggcggagac gctcgccatt gctttggccg agatcccgcg agaaattccg
     1321 ggtgtagaca cggtcgagct gttggtcatc gacgacggca gcagcgacaa cacagcggaa
     1381 attgcgcggc gcgaaggcgt tcaccacgtg gtgggctttc ataccaacca ggggctcgcc
     1441 aaagccttca tgctcgggct tcacgcctcg ctagagcgtg gcgccgacat catcgtcaac
     1501 acggacgctg acaaccaata caacgccgcc gacatcccgc gcttgatcca gcccatcctg
     1561 gagcaccggg cggacatggt gattggcgcg cgccccatct cggaaatcgc gcacttctct
     1621 cccatcaaga agatcctgca gcggttcggg agctgggtcg tgagacgggt cagcgggaca
     1681 gacgtcgcgg acgcgcccag cggttttcgc gccatctccc gcgaagcggc gctgtcactc
     1741 aacgtgttca acggctacac gtacacgttg gagaccatca tccaagcggg acagaagggc
     1801 atgagcgtcg tctccgtgcc tatccgggtg aacggtgagc tgcgcccgtc gcgcctggta
     1861 cgcagtatcc cgaactacgt aaagcgctcg atcctcacga tgatccgcat cttcgtggtc
     1921 tatcagccga tgcgcttctt cattcggctg ggcttgatcc ccttcacgct cggcgtgctg
     1981 attggactgc gcttcttggt ttacttcttc aacggacaag gcacggggca cgtccagtcc
     2041 ctcatcctga ccgccgttct gctcctgatc ggcatccaga ccttcctgct cgcgattctc
     2101 tcggacctca ttggcgtcaa ccgccgactt ttggaagagc tccagcgtcg cgagcgcgca
     2161 cgcctcggct tccgcgccga actgggcgaa aaaccctcga ttccccccct ccccgaccga
     2221 cgaagttcac gtccgccgcc cattggcctc ggcaccaacc gcgaaggcac tttcaacctc
     2281 tgagccgaac cgtcgggcac ccaactagaa cccctggcac ctaaaccagg cgtgctatgc
     2341 tggcgcccgt tcgcacgagt ggcggaactg gcagacgcac cggatttagg ttccggcgcc
     2401 gcaaggcgtg tgggttcgac tccctcctcg tgcacaagca agcagcgcgt tcagggctcg
     2461 tcgctgtcgg agcgacgaag caagcggctg ccttcgaata cggtcaacac gcagacgccc
     2521 gtctcgtccg ctcggtagac gatgcgatag gttcgcaagt acgtctcgcg tatctcggcg
     2581 cgtgcgagct ctgggactat cctcccgagg aggggtgcct cgctcgcgtc ttcgacacgc
     2641 tcgatgagtc gaacgatcca gcgttccgca gcgggtcggt tctcgacggc gatacgcgcg
     2701 aagatttccc gcaggtccgc ttccgcgcgt ttggaccagg ttactgccac gcgatgggcc
     2761 cgaactccgt ttcgagcgac ttcttcaagt cttgtgtcga caggaccctg ccggcatccg
     2821 cgtcagcgag gccctcttcg atcgcggcca cgaagcgctt gtgctctcga agtagagcga
     2881 actcctccgc gtccacgagc accgctgcag gctggccgtc ctgaacgacg acgaccgctt
     2941 cacctgtctc atggacgcgt ttgacgatct ccgccgcgcg ctgcgagagc tcggcggtcg
     3001 tgacgggtgt cttatcagtg agggtcttgg cagcccgtgc ctgctgctct gcacgtcgcc
     3061 gttctgcagc gtccattagc ggagtatagc cgcaaatgca gtgaatgggt gccaacggca
     3121 cgtctgcttc caaagccatc ccgcgtatgc cgcgcgccgt cccaggcctt cggggcgaga
     3181 cttcgccacc tgcgcggtac catagaagct tcctgagaac ggtgtcctcc ggggacttga
     3241 gcacgagtga ctctccagtg ctgcggacga tcacactgcg gcgccgcgca aactcgctgt
     3301 cgtcgaaagc ctcgtacgcg cgaccgaaga aatcgatctt cgtcacgtac ggcaaataga
     3361 acgcgttggc ggtgcgcgcg tggagaatgg catcgcggag catgctcgta tccagctcga
     3421 attcatctcc gagcgcgtcg cgaaacgctt gaacgcgacc ggccggcatc tcgatcacga
     3481 agtcgatgtc gtggtggctc tcggctcgcc ctgcaaggag ctcgcgatgc tcccgcctac
     3541 gaagtagttg gcgcccacgg cctcgagcga cgtcgctacc cgcagcgcga cgtcgatgac
     3601 gtcgaaagtt gcgtcagcgg ccatcgagat tcacgttcgg gaataggcgg ctggcgactt
     3661 cgcgtccgta catgcgcgcg gcgaggtgcg ctcgaacctc ttgctccgtc gcgcccgggt
     3721 gtccgtcgcg aatgccagcc tcagccaagg cacgcaccgc cgagcaaagt cgcgcggctg
     3781 cagcgagacg cgccgcagga ggctgccgcc gcatcagctc gaagtagcgt tcctcggctt
     3841 cgggtgacgt gtcgcgcatc gcctccgagt atacctgccg cggagcgccg ggctgagacc
     3901 aacgccgcgc ccggcagggc gatcgatcga gaaatcccct cccaatcgtc gacccggcac
     3961 ggaacgtggc caacggcggc aaactgaacc gcaggccggt ctttcgcgac atctgacggg
     4021 tgccatgtac ccagagctgg agcggacaga actgccgagc ggtcgcgcgg aaccgctcat
     4081 tggagctagg gccagcgcgc acagcccaac gctcgtcacg gggaacgttt gcgcgttact
     4141 cccgcatccc cgaactcggc ttcccgggac tgaaccgcca aggcgcggca aagtagaacc
     4201 gctcgttgcg tcctcagttc cactccaccc gtgggcttga ccacgggctt tgaatcgtct
     4261 ttgaatcgtc tacgggctgt atcgagatgt cgccagcggt agcgaactgg aacgcgaa
//
LOCUS       DASTKU010000773         5856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1714741, whole genome shotgun sequence.
ACCESSION   DASTKU010000773 DASTKU010000000
VERSION     DASTKU010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714741"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /gene="serC"
                     /locus_tag="VFQ61_26975"
     CDS             <1..640
                     /gene="serC"
                     /locus_tag="VFQ61_26975"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HET9958179.1"
                     /translation="NETVEGLQFHRVIGRDDVPRICDMSSDFMSRPVQMERFALVYAH
                     AQKNLGPSGVTLVLVRKALLERCNAGLPSMLDYRSHMTHGSIFNTPPVFAIYVTLLVT
                     RWLLDEIGGLERMDVINREKAAALYRVIDRSDGFYSARAHADSRSLMNVVFRLASPEF
                     ESEFLRAAEHAGLYGLAGHRSIGGIRASLYNAVALESVHRLCQMMEDFQAAH"
     gene            771..2012
                     /locus_tag="VFQ61_26980"
     CDS             771..2012
                     /locus_tag="VFQ61_26980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958180.1"
                     /translation="MDARSLGGAWDAARGELGRGAKKSGWAIVLVAGFGACTWHDDEA
                     RLQGPPNALAERSFASLWLLDGCAGGGKVNFCSTETVEEVLEQSIDDPRVLRFAEANE
                     VPSGLPADTTHLVVQGLSAGTTKVRVKARFSDGERTTELQVNVAKVDAIQLRSSCAED
                     QAALRVLPGKVLALQVEPQNAGKPLDGFLPELLRGAGVSCTALSWASRMTCSWTAPLE
                     GGAVDLTSGVLPRFARTIRSYGPSDVKAIRAPLPRSAEPIVGPGWTVRLPTATVLEDG
                     VPCESLPVHAETLTPSVCTGPEGTPAWDASTSYELSVKILQTGACKLKLGAVGANTLP
                     STTEFSMRVASQLTADHLAFPETPCSVEGATTCKTFYSAMVCENGRWRDSKTCAKGFC
                     EFVLSGDRGCAHPDGCATCQE"
     gene            2049..2546
                     /locus_tag="VFQ61_26985"
     CDS             2049..2546
                     /locus_tag="VFQ61_26985"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HET9958181.1"
                     /translation="MVAWAAASEVLGAMITPALLISASGTLVLSTSARLSRVVDRVRV
                     LARDAEQLASADHAPNLAERRNLIVSQLHGLAKRALILRSALAALYSAIGFLVATSIG
                     VGILTLLGWKGGWLAIGLAMLGACSLLWGSVLLVREGRLAIGSTLEEMSYVRRVIGSL
                     PEDTD"
     gene            complement(2567..3418)
                     /locus_tag="VFQ61_26990"
     CDS             complement(2567..3418)
                     /locus_tag="VFQ61_26990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02452 family protein"
                     /protein_id="HET9958182.1"
                     /translation="MNRSDRLRVAHETLEIVKRGEYLTRRGDLVSIREAVRECERGTE
                     VLLPDALEGLSRTLLSNVRNGSPAQLTVVHETTLQGIARLHAESPRGIAVLNFASARN
                     PGGGFLNGSQAQEESLARSSALYSSLQCAPAFYEHHRQLSSLLYTDRMVLSPDCPVFR
                     DDRGELLPSPQLVTFITSPAPNAGAIAENRPEELALLDETLRRRSEYILALAASRGLS
                     RLVLGAWGCGVFRNDPARVASAFVTHLRGAWAYHFRQVTFSVFDASPARDTLRAFELA
                     LSTSSRP"
     gene            3564..3722
                     /locus_tag="VFQ61_26995"
     CDS             3564..3722
                     /locus_tag="VFQ61_26995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958183.1"
                     /translation="MSAVMAYRQAKCLLDIVNGRLGKRDSALFFTPQYLAGKPSPNKP
                     AALITLTS"
     gene            3976..>5856
                     /locus_tag="VFQ61_27000"
     CDS             3976..>5856
                     /locus_tag="VFQ61_27000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET9958184.1"
                     /translation="MNHSKRLIRNSYTFSALLIGIAAGSGLISCAEESAPGLGDREST
                     VEASGDAVSTHGAKVDGVRVNAANGERVDAARVESSAAAQGSPQSIVRRGALTPKSAV
                     GAALHRRARPISTETVALTLGAVPAHLQQKALGDSAWREWLQGALGDHVKVLSARPLD
                     PTQVGAPAQGTQVVVQVESTTERNIQSARECATQTRGIARAELDPIWSSNAVPNDPEY
                     GKQWHLPKISAPAAWDVANGSPSVVVAVIDTGIDLTHPDLAGAVWNNPAETADGIDND
                     LNGLVDDVHGWNFLGNNANLADSTGHGTHVAGLIGAVKNNSIGVAGVASGVKIMPLAV
                     GEFAPASAVASAIYYAVANGAHIINMSFGGPDTFSGARAAIDYAVAHGVLLVSSANNK
                     SSDEYNYPAVYQEVLAVAATDPDDKRASLSSYGSWVDIAAPGQAVVSTFPGGGYMAVS
                     GTSQASPIVAGVAALIKSVHLDWSAEQIRTQLMASADDLSATNPDYVGLLGAGRVNAA
                     RAVGPTLTTPRPFLAGVTSSELTGNGDQHVGPGEMASVSVAWRFTGNASAATARLVST
                     DPYVTVTSGPVTLGAPRADRTQTARFTINIAANTPADRLANLTASVDSAGTTFSSTVQ
                     LSV"
BASE COUNT         1088 a         1771 c         1899 g         1098 t
ORIGIN      
        1 caacgagacg gtggaagggc tacagttcca ccgcgtcatt ggcagggatg acgtgccgcg
       61 catctgcgac atgtcttcgg acttcatgtc acgacccgtg caaatggagc gtttcgccct
      121 ggtatacgcg cacgctcaga agaacctcgg tccgtcgggg gtcacgctgg tgttggtacg
      181 caaggcgctg ctcgaacggt gcaacgcggg cctgccaagc atgctggact atcgctcgca
      241 catgacccac ggctccatct tcaacacccc gcccgtgttc gcgatttacg tgactctgct
      301 ggtcactcgt tggctactcg atgagattgg cggtctcgag cgcatggacg tcatcaatcg
      361 ggagaaggcg gccgctctgt atcgcgtgat cgatcgcagt gacggattct attccgcgcg
      421 ggcacatgcc gatagccggt cactcatgaa cgtggtcttt cggctcgcga gccccgagtt
      481 cgagtcggag ttcttgcggg ccgccgagca cgcggggctt tacgggttgg cgggccaccg
      541 gtcgatcgga gggatccggg cgtcactcta caacgccgtg gccctcgagt cggtccatcg
      601 cctctgccaa atgatggaag acttccaggc cgcacattag cccggcccaa ccaggacact
      661 cacagcacgg gggtgtttac tggcgttcac accccgaagt cagaaccctc gcgattggtc
      721 gtcttcggcg cggcgtggac tttgccaaac cgctgagtag catggcggca atggatgcga
      781 ggagtcttgg tggcgcatgg gacgcggccc ggggtgagct cgggcgggga gcaaagaaga
      841 gcggctgggc aatcgtgctt gtcgccgggt tcggcgcgtg cacgtggcac gatgatgagg
      901 cgcgtcttca gggcccaccg aacgcgttgg cagagcgcag ctttgcgagc ctatggctgt
      961 tggatggctg cgcgggtgga ggcaaggtca acttctgcag cacggaaacc gtggaagaag
     1021 ttctagagca atcgattgac gatcctcggg tgctgcgctt tgcagaggcc aacgaggtac
     1081 cctccgggct cccggcggac acgactcatc tagtcgtgca aggcttgtcc gcgggcacga
     1141 ccaaagtgcg cgtgaaggca cgtttcagcg atggagagcg cacgacggag ctgcaagtca
     1201 atgtggccaa ggtggacgcg atccagttgc gctcgagttg cgccgaagac caggcggcgc
     1261 tgcgagtatt gcccggcaaa gtcctggccc tccaagtgga gccgcagaac gccggtaaac
     1321 cactggacgg attccttccc gagctattga gaggggcggg cgtaagctgc acggcgctca
     1381 gctgggcctc gcgcatgacg tgctcctgga cggctccact cgaggggggc gccgtcgacc
     1441 tgacttcggg cgtgttgccc cgcttcgccc gcaccattcg cagctatggc ccgagcgacg
     1501 tgaaagctat tcgggcgccg cttccgcgca gcgcagaacc gatcgtgggc cccggatgga
     1561 cggtgcggct acccaccgcc accgtgctgg aggacggggt accctgcgag tccctgcccg
     1621 ttcacgcgga gacgctcact ccttcagtct gcaccggccc ggaaggaact cccgcttggg
     1681 atgccagcac ctcctacgag ctttcggtca agatcctcca gaccggagcc tgcaaactga
     1741 agttgggtgc cgtcggcgcg aacaccctgc cgagcaccac ggagttttct atgcgagtgg
     1801 ccagccagct gacggctgac catctcgcat ttcccgagac gccctgttcg gtggaaggag
     1861 ccacgacctg caagaccttc tactcggcca tggtctgcga gaacgggcgt tggcgtgatt
     1921 ccaagacctg cgccaaaggc ttttgcgaat ttgtcttatc gggggaccgt ggctgcgctc
     1981 atccggacgg ttgtgcgacc tgccaagaat aggccatcga gtaccgcggc acgaagtata
     2041 ctcacaacat ggtcgcttgg gccgcagcat cagaagtctt gggggccatg atcactccgg
     2101 cgctgttgat ttcggccagt gggactttgg tgctgtcgac gtcggcgcgt ctgagtcggg
     2161 tcgtagatcg agtgcgagtg ttggctcgcg acgcggagca gctcgcgtct gcggatcacg
     2221 cccccaattt ggcggagcgt cgcaacctaa tcgtcagcca gctccacgga ttggccaagc
     2281 gcgccctgat tttgcgctca gcgctcgccg cgctctacag cgccatcggt ttcctggtgg
     2341 ctaccagcat cggcgtgggc atcctcacgc ttctgggctg gaagggaggc tggcttgcga
     2401 tcggcttagc gatgctcggc gcgtgctctt tgctctgggg cagcgtgctg ctcgtgcgcg
     2461 aggggcggtt ggcgatcgga tcgacgctcg aggagatgag ctatgtgcgt cgggtgattg
     2521 gctcgcttcc ggaggatacg gattgagcga gctgctccat tacgcactac ggccgtgaag
     2581 acgtgctcag ggccaactcg aacgcgcgca acgtatctcg agccggtgag gcgtcgaaaa
     2641 cggagaaggt gacttgcctg aagtgataag cccaggcgcc acgcagatgg gtcacgaacg
     2701 ccgaagccac gcgagcggga tcattccgga atacgccgca gccccaagct ccgagaacca
     2761 atcgagacaa cccgcgggag gccgccaacg cgagtatgta ttccgagcgg cgccgcaacg
     2821 tctcgtccaa gagagcgagc tcttctggac gattttctgc aatcgcgccc gcattcgggg
     2881 cggggctggt gatgaaggtc acgagctgag gtgagggtag gagctcgccc ctatcatcgc
     2941 ggaaaacagg acagtccggg gagagcacca tccgatctgt gtacaggaga gagctgagtt
     3001 ggcgatggtg ctcgtagaac gctggcgcgc actgtaagga ggagtagagc gcggagctcc
     3061 gcgccaagga ttcctcttgg gcttggctgc cgttcaagaa tccgcccccc gggtttcgcg
     3121 ccgacgcgaa gttcaagacg gcaattcctc gagggctctc cgcgtgcaag cgtgcaatac
     3181 cctgcagggt tgtttcatgc acgaccgtga gctgagcggg gctaccattt cgaacattgg
     3241 acagcagtgt tcgacttaac ccttcgaggg catctggaag taggacctcg gttccgcgct
     3301 cgcactcgcg aacggcctct cggatcgaaa ctaagtcacc tcgacgagtg aggtattccc
     3361 ctcgttttac tatctcgagc gtttcatgtg cgactctcag ccggtcggaa cgattcattg
     3421 cgtctctctc gaatcttgcc cttcacgagt gtgccgtaca tccgcagccc cgtcacgtcg
     3481 aacggccgcg gtgggccggg taatgccccg gactttttcg tgaatggaag catggcaatg
     3541 gcgttggctc tgcccgcgga tcgttgtctg cggtgatggc ctatcggcag gccaagtgtc
     3601 tcttggatat cgtgaatggt cgccttggca agcgtgactc cgctctgttc ttcacgcccc
     3661 aatacttggc gggcaagccg tcaccaaaca aacctgccgc actgatcacc ctgacgagtt
     3721 gaacgtcacg tgaggtgagc gccagtgaac ctgcaagttg aagtcgcttc ttacgaacgc
     3781 gaggcgaagc ccgcggggga cagggccgac gctcattcta gaaaagtatc gggggatgcg
     3841 aaggtgggac tcgggtccta tctctaatgg ggcgagacca aacctacttg ggctaacgca
     3901 ccgagtgaga ggggtgcgtc ctccaacttg gcgcgtgatc aaacgcggga gaatgcggtg
     3961 tcctatccaa atcagatgaa tcattcaaag cggctaatca ggaactccta cacgttctca
     4021 gctcttctca ttgggattgc ggcgggttca gggctcatct cgtgtgcgga ggagagcgcc
     4081 ccaggcttgg gcgatcgaga gtccaccgtc gaggcgtcgg gtgatgcagt cagcacgcat
     4141 ggggcgaaag tagacggagt tcgggttaat gcggctaacg gcgaacgagt agacgccgcc
     4201 agagtcgagt cgagcgcggc cgctcagggc tcaccgcagt cgattgtgcg tcgaggggca
     4261 ttgacgccga agagcgcggt tggagccgca cttcaccggc gggctcggcc catttcgacc
     4321 gagaccgtcg cccttacgct gggcgcggtg cccgcccacc tgcagcaaaa agctctcggc
     4381 gattcggctt ggcgagagtg gttgcaaggg gccttgggcg atcacgtaaa ggtgctttca
     4441 gctcgtccgc tcgaccccac ccaggtcgga gcaccggcac agggaactca agtcgtggtc
     4501 caggtcgagt ccacgacgga acgcaacatc cagagtgctc gcgagtgcgc gacgcaaact
     4561 cgaggtattg cgcgtgccga gctcgatccg atctggtctt cgaatgccgt cccgaacgac
     4621 cccgagtatg gaaagcagtg gcacttaccg aaaatcagcg cgcccgcagc ttgggacgtc
     4681 gcgaacggct caccaagcgt ggtcgtggcc gtcatcgata cgggtatcga tctcactcat
     4741 ccggatttgg cgggcgccgt ttggaacaac cccgccgaga ctgccgacgg gatcgacaac
     4801 gatctcaacg gtctcgtcga cgacgttcat ggctggaact tcctgggcaa caacgccaac
     4861 ctggcagact cgacggggca cggtacgcac gttgcggggc tcatcggcgc cgtgaagaac
     4921 aactctatcg gcgtggccgg tgtggcttct ggcgtgaaga tcatgccgct cgcggtcggc
     4981 gagttcgctc ctgcgagcgc ggtcgcgtcg gccatatatt atgcggtggc caacggcgcg
     5041 cacatcatca acatgagctt tggcgggccg gacacgttct ccggcgcgcg ggcagccatc
     5101 gattacgcgg tggcgcacgg cgtgctcctg gtttcgtcag ccaacaacaa gtctagcgac
     5161 gagtacaact acccggccgt gtaccaagag gtactcgcag tggccgccac ggatccagac
     5221 gacaagcgcg ctagcctctc gagttacggg agttgggtcg acatcgcggc tcccgggcaa
     5281 gcggtggtga gtacgttccc gggcggaggc tacatggccg tctcgggtac cagtcaagcg
     5341 tcacccatcg tcgccggagt ggctgcgttg atcaaatccg tgcacctcga ttggtctgcg
     5401 gagcagatcc ggacgcagct catggccagc gccgacgatc tgagcgccac caatccagac
     5461 tacgtggggt tgctcggcgc gggtcgggtc aacgccgcgc gggccgtggg cccgacgtta
     5521 accacccccc gcccattctt ggccggggta accagctccg agctgaccgg aaatggtgac
     5581 cagcacgtcg gaccaggaga gatggcctcg gtttcggtcg cctggcgctt cacgggcaac
     5641 gcgagcgccg cgacggctcg cctcgtgtcc acggaccctt acgtgaccgt cacgagtggt
     5701 cctgtgacct tgggcgctcc ccgcgcagat cgcactcaaa ccgcgcggtt tacgatcaac
     5761 atcgcggcca atacgccggc cgatcgcctc gccaatctca ccgcgtccgt cgacagcgca
     5821 gggactacgt tctcctctac cgttcagctc tcggtc
//
LOCUS       DASTKU010000774        17245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1715479, whole genome shotgun sequence.
ACCESSION   DASTKU010000774 DASTKU010000000
VERSION     DASTKU010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 17245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17245
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715479"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1914
                     /locus_tag="VFQ61_27005"
     CDS             145..1914
                     /locus_tag="VFQ61_27005"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET9958185.1"
                     /translation="MIRRSVPCLGLPSARLSLWFLGLLWLGACAADANPGSFVRRRAD
                     IVVDAPSPAPAPSPPSFPVAEPARPLPELTRPPSEPPNPVPEPSAPVPEPAAGTSTQA
                     EPADSASAPVAGVEGGEGGARSEEAVPSVPETRPSLDRQLPDTVLDVSSLPPGPGIPQ
                     LVVVQREAFVYQEPNLESRRIGYLRAGTSVPRSSEPVSRVGCKLGFYRVAPEGYVCRN
                     SAVRLNGGDLVSELSQVRAERGNALPYVYVRSTNPPPPLYTKLPSGAEQREAEPESLV
                     RRRLSKSFEAVSVAAQAPAPLLAGKSVPTPFGGSYGAERAHLGRALGDSTYALLQVFQ
                     HEQRVFGLTRDLLLIPLDHTERVLESTFHGVAISEAVSAVGFVQRRGALAYVSEQPGA
                     LRIARNLAYREALMLSGNEQRISGLRYLETASRELVRADQLLRVELNAELSPAAQRAS
                     TYLRVSIANQTLIAYAAGRPAYVTLVSTGRDGAQDPKTTRSTPTGQFRIHTKHVSTTM
                     SSDEVGDEYRLEVPYVQYFSEGYALHTAYWHDAFGSPRSHGCINLSPIDARHLFNWTE
                     PPVPQLWHGAMSLNEGTLLEVVP"
     gene            2134..3936
                     /locus_tag="VFQ61_27010"
     CDS             2134..3936
                     /locus_tag="VFQ61_27010"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9958186.1"
                     /translation="MKTADLREALGPDLSEALEGKGYEALTPVQVAVLQPELENRDLR
                     ISSQTGSGKTVAIGIVLRSYLSKSAPVRGRSMPTALVVTPTRELAKQVQDELTWLYAP
                     RGVRVAAVTGGANYRDEHRSLAGNPGVVVGTPGRLLDHLRRGSIEAEAIKAIVLDEAD
                     RMLDLGFREDIDAILAFAPEGRRTHLVSATFSREVLSLADSAQQDAMHVQGTPLGTAN
                     QDIEHLVHVVQPRDRLNALVNLLLAEPGSQTLVFARTRADVADLSDELRAAGFSVGSL
                     SGEMEQTQRNRALSAFKRGELRVLVATDVAARGIDVRDVSRVVQMDPPPDPESYTHRS
                     GRTGRAGRKGTSSLVITPSALGKTRMLLQRARVKFRFMPVPTAADLRRQQEEHLFNEL
                     VAEPAEGDTGPSELVRSLAKRLLESDKAELALARLLESSERGARVTPRELTPVDVNVE
                     KTRRRGERPLPFAGKPERFAKPPRGLSVGPLAAEMPPRRVARDRQGPGFEPFRVSYGE
                     NHGADARRLLAMLCRRGGIESADIGAIRVMPTFSLVEIAAEVAGHFERSASMPDPRDR
                     HVRIERDGGAPNYSRGPVRPEFKGRPRKQFAADN"
     gene            complement(4178..4768)
                     /locus_tag="VFQ61_27015"
     CDS             complement(4178..4768)
                     /locus_tag="VFQ61_27015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958187.1"
                     /translation="MRHLAIFGMLTVALFLGSSPAAAQSAGTPRVGATSVGTGSAGLP
                     TGSSAIPAQSGLTASGGTLARVPSIQRLFKLEIPGLQPLHFSFSDAPTPGYELLRLPT
                     FEARATLWSTDETFGRGQLEVSLFERTAPALELDCMLTCAPILEQAAGTDFRFGLGAI
                     TPKIPSSYVFVQPQAMRLPRGFGSRSLLGFGGLLDF"
     gene            complement(5031..7457)
                     /locus_tag="VFQ61_27020"
     CDS             complement(5031..7457)
                     /locus_tag="VFQ61_27020"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9958188.1"
                     /translation="MSEGPLRDPKLLEIESKLNTGSFDEAQRLLADVDSSAQGKLAAA
                     YFSTRLLFQKGRLDRAAVIQRLRDLLGHGLHFPEAERMLLAAEAGTLRPEPDQFRRST
                     GVPSSPLPPRGASSKLTPPTASVRAGDAAVDELQARAASRADSTHTPPASAPARARSV
                     APAASVPATSRGAMAASAAPPDSAQAASEPFPQRIPRAPMVPHFTPRSGIPSYVPSER
                     RATTPEPPVPSTPARRLPSIPSLDVPAPPAIPPSRFPLSERATGSRRPPPEGSRVSAE
                     TAIKSARALLSVGDKPRAAREIERLLGGYALEPPIRTSCARLLIESGRAERGLAEAHR
                     AFAEAPDDPSTRLTCAWALVRVLRRTGDPSVADEVDDLLASTPLQDRLQDGNPSALLL
                     SLYATLAAERGDASRAKSLAQSALQQDPQQVDALAALALALARLGHLEQAEQAQRRLQ
                     ELDPEEAHANQIALARFQAQGLAAPPQSDSAVRAQLARLFGAEEANLSRGQTALVQRA
                     LETACIHRSAKLQRRTLPAAWAALGSSAAKLLTTLPLFRHFAPYDCSLYSVSRLDAAL
                     SLVYGGADNSEPPRSVLELLGSYVGESLRQAFGGEWHVPRPDPLAATVQAAGLSIHPF
                     EQVQSRIQAAEPLPIPRLTRLHPGADPLGNSVPLSIAPPAPWDPDPFPDTATFQLLGR
                     LLPHSVIGFYSEEIEQMPLDFSIASLSALDSYLALLAPENSIPDPDSAWSRRAAVLIG
                     AYVGEVLARVHSAVLQAPREIAGLDAYRYRLPNGVSAAPVTRVLQRLDGRRASSLVEY
                     VTRLAESRGS"
     gene            complement(7641..9785)
                     /locus_tag="VFQ61_27025"
     CDS             complement(7641..9785)
                     /locus_tag="VFQ61_27025"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HET9958189.1"
                     /translation="MTARDPLSIVGQTIADKYLIESMVGEGGFAVVYRAIHRIWNKPV
                     AIKFFTGLSMAPVEHRKQFEQAFIQEGALLTELSSQTATIVQARDIGTYVSADGAWVP
                     FMVLEWLEGATLEHVLEDARVRGARAYTLDSLLPILGQVAHALDVAHQRGIAHRDVKP
                     ANLFLLAPAFTVVKLLDFGVAKMVTDNTQLQAAMARTGMGITSFTPQYGAPEQFNRSV
                     GATGPWTDVFALALVAVEMLIGRPALDGEDVIQLAVSAGRPDQRPTPQSLGVNVSPEV
                     EAVFAKALAVRPQDRYARARDFWTALEAATQAPAQQRIGSGLDVTELAPLNITAPIPS
                     HAPQTAAASRMPLPQPAAPPVTQSPSLRPMAPTTSGNATLNTTQASAKARSHGALFAA
                     GLGVLAVIGAGVFLFNVRNSPQGAASASAERANQEHAAAVLPAAAASSASPAAAPEPS
                     CPERSVKIAAGQFYQGSDRKDALPREQPAHNVTLKAYCIDLYEVTAGEYKACSDQGQC
                     KRASTEVDWPNITPADKARYSPVCTVNDPERKDHPINCVSWEMADRYCKVQGKRLPTE
                     AEWEYAARGPDGRVYPWGDEPPTAQHLNACGKECAAWGRSHGAVLEPLYAADDGFPTT
                     APVGRFEAGRSRFGPYDIVGNVWEWVADYDGPYTAEDKQNPTGPASGDRRVIRGGGWN
                     GSDKSWLRPSYRYAQAPEALSHGVGFRCVKSL"
     gene            complement(9870..10778)
                     /locus_tag="VFQ61_27030"
     CDS             complement(9870..10778)
                     /locus_tag="VFQ61_27030"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HET9958190.1"
                     /translation="MTLAMELLFADLIFGPELDLENSEWVSTWLDRYALDPGDRATLE
                     GGELKRLVVYRSLVRGRLKEALELQMPRLCALLGEQFEPWFGRFLAERGPRTQYLRDV
                     AVEFLDFVAPHWEATPGLPPYWIELGRHEALHVEISALPVAARSWGEPAALSLEAGLQ
                     FCEAARLVRYRHAVHRWLDDGTQPEPEATPIALLAYRDAEHDARFLELSPLAATLVEN
                     LMNGLPLGAALTQALARHQVLLAEARPEIVHELLEQCSVLLADLSERGVILAASSTPT
                     SSTPTSSAPPSSAPANSARYETGLPD"
     gene            complement(10775..11662)
                     /locus_tag="VFQ61_27035"
     CDS             complement(10775..11662)
                     /locus_tag="VFQ61_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HET9958191.1"
                     /translation="MATGPFRGISAPTGVGLGLRWEFLEEIVDGPTLALPFLEVSPEN
                     YMRRGGYFPAQLQKIRERYPLFTHGLTLSIGGESAPRADYLSELAGEIARLRAPFHSD
                     HLCVSGLPGRSTHELLPLRFTRSAARRIAERVARVENELGVPLALENISYYAHPGRHE
                     LTELEFIEEILEHSKAGLLLDVNNVYVNALNHSAGSHEAAYAFLERVPYDRVVEIHVA
                     GHTRQPDGAVIDTHGAPVIPAVHELLQYAVTQSGPIAVLLERDNDVPPLSELMAEVEQ
                     LRAVYERAIAASRAPRRGS"
     gene            complement(11669..12070)
                     /locus_tag="VFQ61_27040"
     CDS             complement(11669..12070)
                     /locus_tag="VFQ61_27040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958192.1"
                     /translation="MHQKLTLQQFRATLAVLGASASVLACSANPPPEAESPATATPPA
                     AEAAAATPGTAGADASSTSASMPAAEAAAPTSPATAEAAAAPTPSAPEPTATDPAKAT
                     ATEVKPAPKAATPAKHKAAGAKGGCGAGTCG"
     gene            complement(12220..12984)
                     /locus_tag="VFQ61_27045"
     CDS             complement(12220..12984)
                     /locus_tag="VFQ61_27045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958193.1"
                     /translation="MRTEPKKKAPTRTEPWPAPLVSTTKPGQTGRLRYRVERAQVEIE
                     LTHRKRTEHARLRAVEGTIDLDPVKLRETRAELRADLLDLSFFQGSEEAPDVTLRALE
                     VLGLDRQKSSATRELDRFVTLQITGFDAVPSQEASRANRATVTARGDLTLHHFRVPVS
                     LELEVELDRKTADSESDVTAQRLLIRTRRPLVVSLTAHDLVPNPSTSSADLPAADGVR
                     ARERGKASAGKTVPEFGREVRVSAELAAIPDPAAKP"
     gene            complement(13058..14554)
                     /locus_tag="VFQ61_27050"
     CDS             complement(13058..14554)
                     /locus_tag="VFQ61_27050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958194.1"
                     /translation="MNFTRASRLRRAAQALTAGLVWTAQAGAQLPGNGERIRTHDYAI
                     DLAQTAVLAGTRATGLAGAYVALGEGIDGTARNPAAVALRAPYSYDHLDYDLGVGVTL
                     SSALAGSDVFNSGRRTVVSQNGEEALVFVNLAGTLQLGRWGFAISTDIQEYSLDRSTA
                     RTPNLQQDRLQARFAVTHGMIGYAFHQGELLVGLGIRTTALSVTNANAPQGADESLFN
                     SQGQGFEAGILLRPNLSQYRIGVSLRSAVTARASPEARQLVYAGDPENELFLPKRVAL
                     PWELDVGYALQIGPRPLNPRWLDPRQELEPLRRYFAWRERERERRRRAAALDPEPGRE
                     TAQRALEAELSTEAALDALELGRAEKELHARLRARYEGMERFHLLITGSLDILGPVED
                     AVGVESFLERSVQRSGTRVTFSPRLGLETEVIPNYTRFRAGAYIEPSRFPNNPHAERF
                     HWTLGLDQRVLPWEVFGLWPEGSIWRVSGSLDMSRDYLSWGVALGMWH"
     gene            complement(14551..15627)
                     /locus_tag="VFQ61_27055"
     CDS             complement(14551..15627)
                     /locus_tag="VFQ61_27055"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9958195.1"
                     /translation="MAWVLVLDRPSGDPTQIVAGLPIALRLALDAQAARAEAIVIAPG
                     AENARPALSDARLKLPVVDAAPAGARRVRVPASYVLHRGLFRKLVELDTSSSERDLAT
                     QPFAHGAAWGFDPIDVADAASRARAEKALFRALRKPEDGWTSRYLNRYISLFFSRLLI
                     HTPLTPNQVSVAILGVGLGGAVSAAQGTPGSLMLGAALLQAQSVLDGCDGELSRVSHR
                     GSRRGEWLDTIGDDLSNYGFFTGAGIGLYRMSGSWIYLAAGLLTVVAGVIASGLEYRY
                     LIRIGSGDLLKYPLSQQTNTRREGPLSALAPLFKRDTFVFLTLIAALLGWLGPVLVVF
                     SLAAVGVLIAVIRTELRLAREGAA"
     gene            15853..17178
                     /gene="thrC"
                     /locus_tag="VFQ61_27060"
     CDS             15853..17178
                     /gene="thrC"
                     /locus_tag="VFQ61_27060"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET9958196.1"
                     /translation="MPYSAEFRCFAGCEGSWPVTRPIYRCPTCGGLLQVVHDVDALRD
                     RSGAAWMRLFDERYRQNAWPYGSGVWGKREWVMPQMGDHSIVSMYEGGTNLFWAERYG
                     KMVGLEDLWVKLCGNSHSGSFKDLGMTVLVSVVRMAIQEGLKVKALACASTGDTSASL
                     AAYGAAAGLPVVVLLPRGKISTAQLVQPLAHGALVLGLDTDFDGCMTIVQRLAEEGLV
                     YLANSMNPLRLEGQKTVGIEITQQFDWQVPDWIILPSGNLGNASALYAGFKMMRELGV
                     ISRYPRLVVAQAENANPLYRAWSEGKRQVTPIQAKPTLATAIQIGNPVSAPRAIQALE
                     SMNGLVEQASEDELCEASARADRTGMYCCPHTAVGLAVLEKLVARKVIQPQEKVVCVS
                     TAAGLKFTEFKVRYHDRQIEGVRGEQSNPAVFLPADFGQVVDAIHRRFQ"
BASE COUNT         2989 a         5444 c         5864 g         2948 t
ORIGIN      
        1 ggcgtcacgt tcggagagcg cagcgagcga ccagggaacg gcgtgactcg gagtcatgac
       61 gttcattgaa gcacaccgaa aaccaggacc tgcgcgaaaa acttggccgc gcgcgggccg
      121 ctcagctagg cgcagagaac ctccttgatc cgccgatccg ttccctgcct cgggctgccg
      181 tccgctcgtc tctccctctg gtttttgggg ctcttgtggc tgggtgcgtg cgcggccgac
      241 gcgaatccgg gaagcttcgt gcggcggcgc gccgacatcg tggtcgacgc cccgagccct
      301 gcgccggcgc cctctccacc ttcgtttccc gtcgccgagc ctgcgcgtcc gctcccagag
      361 cttacgcgtc cgccctccga gcctccaaat cccgttccag agccgtccgc gccggtacca
      421 gaacccgcgg cgggcacctc gacccaagcc gaaccggcgg actctgcaag tgctccggtg
      481 gcgggagtcg agggaggtga gggcggagcg cgttccgaag aagcggtccc gagcgtgccc
      541 gagactcggc cgagcttgga tcgccaattg cccgataccg tgctcgatgt ttcctcgctg
      601 ccgccgggac cggggatccc gcaactcgtc gtggtgcagc gcgaagcgtt cgtttaccaa
      661 gaaccgaacc tcgagtcgcg caggattggg tatctgcgtg ccggcaccag cgtgccgcga
      721 agctcggagc cagtatcgcg tgtcggctgc aagctcggct tttatcgggt cgctccggaa
      781 ggctacgtgt gccgcaactc agcggtgcgc ttgaacggcg gagatttggt ttcggagctc
      841 agccaggtgc gcgccgagcg cggcaacgcg ctgccgtatg tttacgtccg ttccaccaat
      901 ccgcctccgc cgctttacac caagctgccg agtggggccg agcagcgcga ggcggagcct
      961 gaatcgctcg tgagaaggcg cctctccaag agcttcgaag ccgtgagcgt ggccgcgcag
     1021 gcacccgcgc cgctgctcgc tggcaagagc gtaccgactc cgttcggtgg atcgtatgga
     1081 gccgagcgag cgcacttggg ccgcgcgctg ggcgacagca cttacgcgct gttgcaggtc
     1141 tttcaacatg agcagcgggt cttcgggctc acgcgcgacc tgctcctcat cccgctcgac
     1201 cacaccgagc gcgtgctcga gagcacgttc cacggggtcg ctatttccga agccgtgtcc
     1261 gcggtcggtt tcgtgcagag acgaggcgcg ctggcgtacg tgagtgaaca accgggcgcg
     1321 ctgcgtatcg cacgcaactt ggcctatcgc gaggcgttga tgctgagcgg caacgagcag
     1381 cgcatctcgg gtttgcgtta cctcgaaaca gcgagccgtg agctcgtacg cgccgaccag
     1441 ctgcttcgcg tcgagctgaa cgcggagctc tctccagccg cgcagcgcgc ttcgacctac
     1501 ttgcgcgtgt cgatcgccaa tcagacgctc atcgcgtacg ccgcgggccg ccccgcctac
     1561 gtcacgctcg tttctaccgg gcgtgatggg gcccaggatc ccaagaccac tcgctccacc
     1621 ccgacgggtc agttccggat ccacaccaaa cacgtcagca ccacgatgag cagcgatgag
     1681 gtcggcgacg agtatcggct cgaggtgccg tacgtccaat acttctccga aggctacgcg
     1741 ctccacactg cctattggca cgacgcattc ggcagccccc gcagccacgg ctgcatcaac
     1801 ctgtccccga tcgacgcgcg ccacctgttc aattggaccg agcccccggt tccccagctc
     1861 tggcacggag ccatgtcttt gaacgagggc acgctgctag aagtagtgcc gtgaacacgg
     1921 ccggaaacac gccactgcaa acggaaacac gctgcaccaa cgcgccggac cggaacgcga
     1981 gagactccaa gcagagctgc aaatcacgtt tcgcatcgag tgtttcggcg cgtcacacga
     2041 gctactgatt ccacccgcag ggtagcgtct tcgaaaataa agagttcaag gaccggccga
     2101 tccgtcggag tcgtgatata gctccgcccc ctgatgaaaa ccgctgacct ccgcgaagct
     2161 cttggtcctg atctgtctga agcgctggag ggcaaaggtt acgaggcact cacgcccgta
     2221 caggtcgcgg tactgcagcc ggagctcgaa aaccgcgatt tgcggatcag ttctcaaact
     2281 gggtctggca aaaccgtggc gatcgggatc gtcttgcgga gctacctcag caaatcagcc
     2341 ccggtacgcg gccgctcgat gccgaccgcg ctggtcgtca ctcccacgcg cgagctcgcg
     2401 aaacaggtgc aggacgagct cacttggttg tacgcgccac gtggcgttcg cgtcgcagcc
     2461 gtaaccggcg gcgccaacta tcgtgacgag caccgcagct tggcgggtaa cccaggcgtg
     2521 gtcgtcggca ccccgggtcg cttgctcgat cacctgcgcc gcggctcgat cgaggccgag
     2581 gcgatcaagg ccatcgtgct cgacgaggcg gatcgcatgc tcgatttggg tttccgtgag
     2641 gacatcgacg cgatcttggc gtttgcgccg gaaggtcgtc gcactcacct cgtgtcggcc
     2701 actttctcgc gcgaagtgct gtcgctcgcg gactcggcgc agcaggacgc catgcacgtt
     2761 cagggcaccc cgctcggcac ggccaatcaa gacatcgagc acttggtgca cgtggtgcag
     2821 ccgcgcgacc gtctgaacgc actcgtcaac ctgctgctcg ccgagccggg ttcacagacc
     2881 ttggtgttcg cgcgcacccg cgccgacgtc gcggatttga gcgacgagct gcgcgcggct
     2941 ggcttcagcg tcggctcgct gtcgggcgag atggagcaga cgcaacgcaa ccgtgcgctc
     3001 tccgccttca agcgcggcga gctgcgcgtg ctggtggcga cggacgtggc tgcgcgcggc
     3061 atcgacgtgc gggatgtctc gcgcgtggtg cagatggatc cgccgcccga tccggagagc
     3121 tacacgcatc gcagcgggcg caccggtcgt gccgggcgca aaggtacgag ctccctcgtc
     3181 atcacgccga gcgcactcgg caagacgcgc atgctgctcc agcgcgcgcg cgtcaaattc
     3241 cgcttcatgc cggtccccac cgcggcggac ttgcgacgtc agcaagagga gcacctgttc
     3301 aacgagctcg tggccgagcc tgccgaaggc gacacggggc cgagtgagct cgtgcgctcg
     3361 ctcgcgaagc ggctgctcga gagcgacaag gccgaactcg cgctcgctcg cctgctcgag
     3421 tcttccgagc gcggagcgcg cgtgaccccg cgcgaactca ctcccgtaga cgtcaacgtc
     3481 gaaaagacgc gccgacgcgg cgagcgcccg ctgcccttcg ccggaaaacc cgagcgcttc
     3541 gcgaagccgc cccgcggttt gtcggtgggg cccctcgcgg ccgagatgcc cccgcgtcgc
     3601 gtcgcgcgcg accgtcaggg ccccgggttc gagccgttcc gcgtgagcta cggcgaaaac
     3661 cacggcgcgg atgcgcgtcg tctactcgcc atgctgtgcc gacgcggcgg catcgagagc
     3721 gcagacatcg gcgctatccg cgtcatgccc accttctcac tcgtcgagat tgccgcggag
     3781 gtggctggtc acttcgagcg ctctgcgagc atgccggatc cgcgcgatcg tcacgtgcgc
     3841 atcgagcgcg acggcggcgc gcccaattac tcccgcggcc ccgttcgtcc ggagttcaaa
     3901 ggccgtcccc gtaagcaatt cgccgccgac aactgatttg cggctccgtt cacggtgagc
     3961 agctggcccc actcgaacgg gcgcgaagcc cgttcgagtg caattcagcg cagcctttcc
     4021 accccaccgc gcccgcacca ccgcgcctgc cccctcggtc cacacgacgt cgtcgtcgcg
     4081 cttcgggatt aggacgggcg aaatccaagc tcagaaggca tccctggttc gtcgacacgg
     4141 cgcgcggagc gatcaaacgc aacccgcctg cacgcgctca aaagtcgagg aggccgccga
     4201 aacccagcaa gctgcggctg ccgaacccgc gagggagacg catggcctgc ggctgcacga
     4261 agacatagct agacgggatc tttggggtga tcgcgcccag cccaaacctg aagtcggtgc
     4321 ccgccgcctg ctcgaggatc ggcgcacagg tgagcataca gtcgagctcg agcgcgggcg
     4381 cggttcgctc gaacagggac acttcgagtt gccccctgcc gaaagtctca tccgtgctcc
     4441 acagcgtggc gcgcgcctcg aaggtcggta aacgaagtag ctcgtatccc ggggttggtg
     4501 cgtcactgaa tgaaaagtgg agcggctgca gcccaggaat ctctagcttg aacaagcgct
     4561 gaatggatgg cacccgcgcg agcgtgcccc ccgacgccgt cagtccgctc tgcgctggaa
     4621 ttgccgagct tcccgttggg agccctgcgc tccccgtgcc aacgctggtt gctccgacgc
     4681 ggggagttcc ggcgctttgc gctgctgcag gcgaactccc gaggaacagg gcgaccgtga
     4741 gcatcccgaa tatagcgaga tggcgcatga accgaggtta ggcctttgtg ggaagatggg
     4801 caatccgtgc ccttgaggca cacgaccgag aacgtgagtt cacgtatgat gacgcagaat
     4861 tggccgctga cggagctggc gttatttcga ggcccgagcg cggttcaatg gggaacccgg
     4921 ggtattgggc ctgcgccagg gggggcgatg acgggcggtc gcgcccgtaa ccgcgagtgc
     4981 gaaactcaaa caggctcgac agttcgacgg tttcaacaca tcgatagagc tcagctgccg
     5041 cgggactcgg cgagtcgagt gacgtactcg acgaggctgc tcgctcgtct gccgtccaaa
     5101 cgttggagaa cgcgcgtgac gggcgcggcc gagacgccgt tgggcaggcg atagcgatag
     5161 gcatcgagac cggcgatttc tctgggtgcc tggagcaccg ctgaatgcac gcgcgccaag
     5221 acttcgccga cgtaggcacc gatcaatacg gcagctcggc gcgaccatgc ggagtcagga
     5281 tcgggaatcg aattttcggg cgcgagcagg gccaagtacg agtccaacgc ggagaggctg
     5341 gcgatcgaga agtcgagggg catctgctcg atctcttcgg aataaaaccc aatcacgctg
     5401 tgcggtagca gacgcccaag cagttggaat gtggccgtgt ccgggaacgg atcgggatcc
     5461 cacggagcgg gcggcgcgat gctgagagga acggagttgc cgagcggatc ggcccccggg
     5521 tgcaagcggg tcagtcgtgg aatgggcagc ggttctgcag cttgaatgcg gctctggact
     5581 tgctcgaacg ggtgaatgga gaggcccgcc gcttgtacgg tggcggctag aggatcggga
     5641 cgcgggacgt gccattctcc gccgaaggcc tgacgtaggc tctccccaac atacgagccg
     5701 agaagctcga gcacgctgcg cggaggctct gagttgtccg cgcccccgta gacgagcgag
     5761 agggcagcgt ccaagcggct gacgctgtaa agcgaacaat cgtagggcgc gaagtgacga
     5821 aacagcggta acgtcgtgag gagtttggcg gcgctcgatc cgagcgcggc ccaggctgcg
     5881 ggtaacgtgc gtcgctgcag tttggcagaa cgatggatgc acgcggtctc tagcgcccgc
     5941 tgcacgagcg cagtttgacc gcgactcaga ttcgcctctt ccgctccgaa taggcgcgcg
     6001 agttgggccc gtacggcaga gtcggattgc gggggcgcgg ctaagccttg agcttgaaag
     6061 cgagcgaggg cgatttggtt ggcgtgggcc tcctcgggat cgagctcctg caggcggcgc
     6121 tgagcttgct cggcttgttc gaggtggccg agtcgggcga gcgcgagggc gagcgcggcg
     6181 agcgcgtcca cctgctgggg gtcttgttgc agtgcactct gggcgaggct cttggcgcgc
     6241 gaagcgtccc cacgctcagc ggccaaggtt gcgtataacg agagcagcaa tgcgctcggg
     6301 ttcccgtcct gcagtcgatc ttgcagcgga gtggacgcga gcagatcgtc cacttcgtcg
     6361 gccacgcttg ggtccccagt gcggcgcagc acgcgtacga gggcccacgc gcaggtcagg
     6421 cgagtgcttg gatcgtcggg cgcttcggcg aaggcgcggt gtgcttcggc gaggccccgc
     6481 tcggcacgac cggactcgat gagtaggcgc gcgcacgagg tgcggattgg cggctcgagc
     6541 gcgtagcctc cgagcaggcg ctcgatttcg cgagcggcgc gcggcttgtc gccgacagac
     6601 agcaaagcgc gggcggactt gattgccgtt tcggcgctga cgcgagagcc ctcgggcggg
     6661 gggcggcgcg atccggtggc gcgctcggag agcgggaatc gagagggggg aattgcaggc
     6721 ggggcgggca cgtcgagcga aggaatcgac ggcaatcggc gcgcaggtgt gctcggaacc
     6781 ggaggctcgg gagtggtggc acgtcgctcc gacggcacat aactcgggat gccgctgcga
     6841 ggcgtgaagt gcggaaccat cggcgcgcgc gggatgcgtt gcgggaatgg ttccgatgcc
     6901 gcttgcgcgc tgtcgggggg agcggccgat gccgccatgg ctccgcgcga ggtggcgggc
     6961 accgatgccg cgggcgcgac ggatcgggct cgcgcgggag cactcgccgg tggtgtgtgg
     7021 gtggagtcgg ctctgctcgc cgcgcgagct tgcagttcat cgacggcggc atcgcccgcg
     7081 cgcacggatg ccgtgggtgg cgtgagtttg gacgaggctc cgcgaggagg aagcgggctc
     7141 gagggcactc cggtagagcg ccggaattga tccggctccg gccgcagcgt cccggcttcc
     7201 gcagctagga gcatgcgctc ggcctcggga aagtgcaggc catggccgag cagatcgcgt
     7261 agccgctgga tcaccgccgc gcggtcgagg cgcccttttt ggaacagtaa gcgcgtggag
     7321 aagtaggcgg ccgcgagttt gccttgcgcc gagctatcca cgtcagcgag caggcgttgc
     7381 gcttcatcga agctgccggt gttaagtttg ctctcgatct ccagtagctt gggatcgcgc
     7441 agcgggccct cgctcatcga gcctcgagca taaaccgacg ggcgaccccg ccgacagttc
     7501 tttccgcgcg ccgggtgaag gcaaacgtag gaacgaatgc gctaaatcac cgcggtgcag
     7561 gctcggggtt cgcagcggaa ctgcgctttc gagcacacgg ggtcaggttg cgcgaggctg
     7621 acggatcctc gagcgcaggt tcagagcgac ttgacgcatc ggaaacccac gccgtggctc
     7681 agcgcttccg gcgcttgagc gtaacgatac gatggcctga gccacgattt gtcgctgccg
     7741 ttccaaccac cacctcgaat cacacggcga tcgccgctcg cggggccagt ggggttctgc
     7801 ttgtcttccg cggtgtacgg gccgtcgtag tccgccaccc attcccagac gttgccgacg
     7861 atatcgtacg gaccaaagcg tgagcgaccc gcctcgaagc ggccgacggg cgcggtcgtg
     7921 gggaagccgt cgtccgccgc gtagagcggc tcgagtacgg cgccatgtga gcgcccccaa
     7981 gcggcgcatt ccttgccgca ggcgttcaga tgttgcgccg tggggggctc gtctccccaa
     8041 ggatagacac ggccatcggg gcctcgagcg gcgtattccc actcggcttc ggtcggcagg
     8101 cgcttgccct gcaccttgca gtatcgatcg gccatctccc agctcacaca gttgatgggg
     8161 tggtccttgc gttcgggatc gttgacggtg caaacgggcg agtaacgggc cttgtcggcg
     8221 ggcgtgatgt tcggccaatc cacttcggtg ctggcgcgtt tacattggcc ctggtcggag
     8281 caggccttgt attcgccggc ggtcacctcg tacagatcga tgcagtatgc cttgagggtg
     8341 acgttgtggg cgggctgctc gcgcggcaga gcgtccttcc tgtcggagcc ctgataaaac
     8401 tgacccgccg cgatcttcac gctgcgctcg gggcagctcg gttcaggggc agccgcaggc
     8461 gatgcgctgg atgcggcggc ggctggtagc acggccgcgg cgtgctcttg attggcgcgc
     8521 tcggcgctcg cggacgccgc gccttgcggg gagttccgca cgttgaacag gaacacgccc
     8581 gcccctatca ctgccaatac accgagcccc gccgcgaaca gtgcgccatg cgagcgagcc
     8641 ttcgcggagg cttgagtggt gttgagcgtg gcgtttccag aagtggtcgg cgccatcggg
     8701 cggagcgagg ggctctgcgt aaccggtggc gccgcgggtt ggggcagggg cattctagac
     8761 gccgccgccg tctgcggagc gtgactggga atgggcgccg tgatgttgag cggcgcgagc
     8821 tccgtcacgt ccaaaccaga gccgatgcgc tgttgcgcgg gtgcttgagt agccgcttcc
     8881 agggcggtcc agaagtcacg cgctcgcgcg tagcgatctt gaggtcgcac cgcgagcgct
     8941 ttggcgaata cggcttccac ctccggcgag acgttgacgc ccaagctctg cggagtggga
     9001 cgttgatcgg ggcggcccgc cgacaccgcg agctgaatta cgtcttctcc gtcgagcgct
     9061 gggcgtccga tgagcatctc cacggcgacc agggccaacg cgaacacgtc cgtccagggg
     9121 ccggtggcgc ctacggagcg attgaactgc tcgggagccc catactgcgg agtgaaagag
     9181 gtgatgccca tgccggtgcg agccatggcc gcttgcagct gggtgttgtc cgtcaccatc
     9241 ttggcgacgc cgaaatcgag cagcttgacc accgtgaacg cgggcgcgag caggaataga
     9301 ttggcgggct tcacgtcgcg atgggcgatg ccccgctgat gcgccacgtc cagcgcgtgc
     9361 gcgacctgcc ccagaattgg cagcagcgag tccaacgtgt aggcacgcgc gccgcgcacg
     9421 cgcgcgtctt cgaggacgtg ctccagagtg gcgccttcca gccactccag caccatgaaa
     9481 ggcacccacg cgccgtctgc cgagacgtag gttccgatgt cgcgcgcctg cacgatggtc
     9541 gcggtctgac tcgatagctc ggtgagcagc gcgccttctt ggatgaatgc ctgttcgaac
     9601 tgcttgcgat gttcgaccgg cgccatcgac agcccggtga agaacttgat cgccacgggc
     9661 ttgttccaaa tcctgtggat cgcccggtag acgaccgcga acccgccttc tcccaccatg
     9721 ctttcgatga gatacttgtc ggcgatggtt tgaccaacga tcgagagcgg atcgcgagca
     9781 gtcattgaat cctcagtcca aggtaaccca tagaagaagc caagaactat gcagtacagg
     9841 gaaggggcgg cgctagccgg cgctagccac taatcgggca aaccggtctc gtaacgtgcg
     9901 gaattagcag gtgcggaact aggaggtgcg gaactggtag gtgtggaact ggtaggtgtg
     9961 gaagaggcag cgaggattac accgcgctcc gataggtctg cgagtagcac cgagcactgc
    10021 tccagaagct catggacgat ctctggccgt gcctcggcca agagaacctg gtgccgagcc
    10081 agggcctgcg tcaatgcggc tccgagtggc aaaccgttca tcaaattttc gaccagggtt
    10141 gcagcgagag ggctgagttc cagaaatcgc gcgtcgtgtt cggcgtcgcg ataggccaag
    10201 agcgcgatgg gggtggcctc tggttcgggt tgggttccgt cgtcgagcca gcggtgcacc
    10261 gcatgtcgat agcgcaccaa tctggcggcc tcgcagaatt ggagcccggc ttcgagcgac
    10321 aaagcagcgg gctctcccca ggaccgtgct gccaccggca gcgccgagat ttcgacgtgt
    10381 agcgcctcgt gccgccccag ctcaatccag tacgggggca gaccgggagt ggcttcccaa
    10441 tgtggcgcga cgaagtcgag aaactccacc gcgacgtctc tcagatattg agttcgaggg
    10501 ccacgctcgg cgagaaagcg cccaaaccaa ggctcgaatt gctcaccgag caacgcgcac
    10561 aggcgcggca tttggagctc gagagcctct ttcaagcggc cgcgcaccag gcttcgatac
    10621 acgaccaggc gcttcagttc tcccccttcg agcgtagccc ggtcgcccgg atcgagggcg
    10681 taacgatcga gccaagtcga aacccactcc gagttttcca gatcgagctc ggggccgaag
    10741 atcaggtccg cgaataagag ctccatcgcg agcgtcatga gcctcgccgg ggggctcgcg
    10801 aggctgcgat cgcgcgctcg tagactgcgc gcagctgctc gacctcggcc atcaactccg
    10861 aaagcggcgg cacgtcgttg tcgcgctcga gcaacaccgc aatcgggccg ctctgcgtga
    10921 ccgcgtattg cagcagctca tgaaccgccg gaatgacggg cgcgccgtgg gtatcgatca
    10981 cggcgccatc tggctgccga gtgtggccgg cgacgtgaat ttcgaccacc cgatcgtacg
    11041 gtacccgctc caagaaagcg taagcggcct cgtgagaccc cgcgctgtgg ttcaacgcgt
    11101 tcacgtacac gttgttcaca tccagcagta aaccggcctt gctgtgctcc agaatttcct
    11161 cgatgaactc cagctcagtc aattcgtggc ggcccggatg cgcgtaatag ctgatgttct
    11221 cgagcgcgag tggcaccccc agctcgtttt ccacgcgggc cacccgctcc gcgatccggc
    11281 gtgccgcgct gcgggtgaat cgtagcggca gcagctcgtg cgtactccgc ccgggcaggc
    11341 cactcacgca caaatggtcg ctgtgaaagg gcgcgcgcag tcgagcaatc tcacccgcga
    11401 gctcagatag gtagtctgcg cgtggcgcgc tttcaccacc gatcgagagc gtgaggccgt
    11461 gtgtgaatag aggatagcgc tcccgaatct tctgcaattg ggcggggaaa tagccgcccc
    11521 gccgcatgta gttctcgggc gagacctcga ggaacggcaa cgcgagcgta ggcccatcca
    11581 cgatttcctc caggaactcc cagcgcaggc cgaggccgac gccggtcggt gctgaaatgc
    11641 cgcggaatgg gccagttgcc atgtgcgctc agccgcaggt gcccgcgccg cagccaccct
    11701 tggcgccagc tgctttgtgc ttggcagggg ttgcggcctt gggtgccggt ttcacctcgg
    11761 tggcggtggc ttttgccggg tcggtagccg tcggttccgg ggcgctgggg gtgggggcgg
    11821 cagcggcttc ggccgtcgcg ggggacgtgg gcgccgcggc ttcggcggca ggcatgctcg
    11881 cactcgtgct cgaggcatct gcacccgctg ttcctggagt agctgctgcg gcttcggcgg
    11941 cgggcggcgt cgcggtggca gggctctccg cctcgggcgg aggattggcg ctgcaagcca
    12001 acacgctggc ggaagcgccg aggacggcga gagtggcacg gaattgctga agggtcagtt
    12061 tctgatgcat cacggatggc tccttgggcg ctttggtacg gaggtgatcg aacttagggg
    12121 ctctgttggg cttgtgggct gtcgagctct gttaggggtg ttgggagcgg gtacgaccgc
    12181 cgcagccgca acagggtacg ccgctcgaag cggtgagttt caaggttttg ctgcggggtc
    12241 tggtattgcc gctagttcag cgctgactcg aacctcgcgg ccgaattcgg gcacggtctt
    12301 tcccgcgctt gccttccccc gctctcgcgc ccgaacccca tcggcggcgg gcaaatcggc
    12361 gctggatgtt gaaggattcg ggacgaggtc atgagcagtc aacgaaacta cgagtggccg
    12421 tcgcgtacgg atcagaaggc gctgagccgt aacatcgctt tctgagtcag cggtcttccg
    12481 gtccaattcc acttcgagtt ccagagacac aggaactcga aagtggtgaa gggtgaggtc
    12541 accgcgtgcc gtgaccgtcg ctcgattggc gcgcgaggcc tcttggctcg gcacagcatc
    12601 gaagcccgtg atttggagtg tcacgaagcg atcgagctcg cgcgtggcgg agctcttctg
    12661 acgatccaag ccgagcacct cgagcgcccg cagagtaacg tccggtgctt cttcggagcc
    12721 ttgaaagaag gagaggtcga gtaggtcggc tcgaagctcg gcgcgcgtct cccggagctt
    12781 caccggatcg aggtcgatcg taccctccac tgcgcgcagc cgagcatgtt cggtgcgctt
    12841 gcgatgcgtc aactcgatct ccacttgggc tctctccact cggtagcgca gccgacccgt
    12901 ttgaccgggt ttggtggtgg agaccagcgg tgcgggccac ggctcggtgc gcgtgggggc
    12961 cttcttcttt ggctcggttc gcacgcacga gatgcctcca aggagcatga aacatcccaa
    13021 aacgtgcagt cggtgtgggg agcgtcgttt catgccttca atgccacatg ccaagtgcaa
    13081 caccccaact caagtaatcg cgagacatat ccagcgagcc gctcacgcgc caaatcgaac
    13141 cctccggcca gagtccgaac acttcccagg ggagcacgcg ctgatcgagc cccaaagtcc
    13201 aatgaaagcg ctcggcgtgg gggttgttgg gaaagcgaga gggttcgata tacgcaccag
    13261 ctcggaaacg ggtgtagttg gggatgacct cggtctcgag cccgaggcgc ggggagaatg
    13321 tgacgcgtgt ccccgaacgt tgcacgctac gctcgaggaa agactcgacc ccgacggcgt
    13381 cttcgaccgg gccgagtatg tcgagcgagc cggtgatcaa gagatggaag cgctccatgc
    13441 cttcgtagcg agcgcggagc cgggcgtgca attctttctc cgcgcgcccg agctctagtg
    13501 catcgagcgc ggcctccgtg gacagctcgg cttcgagcgc gcgctgagcg gtttcgcggc
    13561 ccggttcggg gtcgagggcc gcggcacggc ggcgtcgctc gcgctcccgc tcgcgccacg
    13621 cgaagtagcg gcgcagcggc tcgagctctt gacggggatc cagccaacgc gggttcagcg
    13681 ggcgagggcc gatttgcagc gcataaccca cgtccagttc ccacgggagg gccactcgct
    13741 tgggcaagaa cagctcgttt tctgggtcgc ccgcgtaaac tagctgtcga gcttccggtg
    13801 aagcgcgcgc cgtcaccgcc gaacgaaggc tcacgccgat gcggtactgg gagaggttcg
    13861 gacgcagcag gatgccggct tcgaagccct gcccttgtga gttgaacaag ctctcgtcag
    13921 cgccttgcgg cgcattggcg ttggtcaccg agagcgcggt cgtgcgaatc ccgaggccga
    13981 cgagcagctc gccttgatgg aaggcgtatc cgatcatgcc gtgcgtgacg gcgaagcgcg
    14041 cctgtaaacg gtcttgctgc aggttcgggg tgcgcgcggt ggagcgatcg agcgagtact
    14101 cctggatgtc cgtcgagatc gcgaagcccc aacgaccgag ctgcagcgtt cccgccaggt
    14161 tcacgaacac caaggcttct tcgccgtttt gggagactac cgtgcgtcga cccgagttga
    14221 atacgtccga gccggcgagc gccgaggata gcgtgacccc gacgcccaag tcgtagtcca
    14281 gatgatcgta cgaatacggg gcccgcagcg cgaccgccgc ggggttgcgt gcggttccgt
    14341 cgatgccctc tccgagcgcg acgtacgcac ccgccaagcc ggtggcccgc gtgccggcca
    14401 gtacggcggt ttgcgcgaga tcgatcgcgt aatcgtgagt gcggatccgt tcaccgttgc
    14461 cgggcagctg ggctccggct tgggccgtcc aaacgagccc cgccgtgagg gcttgggctg
    14521 cgcgtcgcag ccgcgacgcg cgcgtgaaat tcacgccgcc ccctcacgtg ccaagcgcag
    14581 ttccgtacgg atcacggcaa tcagaacgcc caccgcggcc agcgaaaaaa ccacgagcac
    14641 ggggccgagc cagccgagca gcgccgcgat cagcgtgagg aacacgaaag tatcgcgttt
    14701 gaacagcggt gcgagtgcgg aaagggggcc ttcgcgtcga gtgttggtct gctgactcag
    14761 cggatacttc agcaggtccc cggagccgat tcggatcaga taacggtact cgaggccgct
    14821 cgcgatcacg ccggctacca ccgtcagcaa ccccgccgcg agatagatcc aagacccgct
    14881 catgcggtac aagccaatgc cggcaccggt gaagaagccg taattcgaga gatcatcgcc
    14941 gatggtgtcg agccactcgc cgcgccggga gcctcggtgg ctcacgcggc tgagctctcc
    15001 gtcgcaaccg tccagcacgc tttgagcttg cagcagcgcg gcgccgagca tgagcgaccc
    15061 cggcgtgccc tgggcggcgc tcaccgcccc acccaaacca acccccaaaa tcgcgaccga
    15121 gacctgattg ggggtgaggg gagtgtggat gagcaggcgc gagaagaaga gcgagatgta
    15181 tcggttgaga tagcgcgagg tccagccatc ttccggcttg cgtaaggctc tgaacaaggc
    15241 tttctcggcg cgcgcccggc tggcagcatc cgcgacgtcg atcggatcga agccccaagc
    15301 tgcgccgtga gcgaacggct gggtggcaag gtcccgctcg ctcgaactcg tatccagctc
    15361 cacgagcttt cggaacaaac cgcgatgcag cacgtagctt gcaggtaccc gaacgcgccg
    15421 agcgcccgca ggtgcggcgt cgaccacggg caacttcagc cgcgcgtcgg acaacgccgg
    15481 gcgcgcgttc tctgcaccgg gcgcgatcac gatcgcttcg gcgcgcgccg cctgtgcatc
    15541 gagcgccaac cgcagcgcga tcggcaagcc agcgacgatt tgtgtcggat cgcccgacgg
    15601 gcggtcgagt acgagcaccc aggccatgag ggcggcgagt ctagctcgaa ggttcggtct
    15661 gtctgcaccc tcctgcgctc gggcagcccg gccgagctcg tgccctcgag cgtgctttac
    15721 tgccggggaa gctcgggttt tggctcggcg gaccccgagc gttcgctcgg ccgacctagc
    15781 caaagctccc gggatcaggt gcgaaagcgc aatgacgagc gtcggagccg ctcgttggtt
    15841 atagtcggcc ccgtgccgta ctccgccgag ttccgctgtt ttgctggttg tgaaggttcc
    15901 tggcccgtaa cgcgacccat ctatcgctgt cccacctgtg gagggttgct tcaggtcgtg
    15961 cacgatgtcg acgcgctccg cgatcggagc ggggccgcct ggatgcggtt attcgacgag
    16021 cgctaccgcc agaatgcctg gccgtacggc tcgggcgttt ggggcaagcg cgaatgggtc
    16081 atgccccaaa tgggcgacca cagcatcgtc tccatgtacg aaggcggaac caatctgttc
    16141 tgggccgagc gctacggcaa gatggttggt ctcgaggatc tttgggtcaa gctgtgtggc
    16201 aacagccact ccgggtcctt caaagatctc ggcatgaccg tgctggtgag tgtggtgcgc
    16261 atggcgatcc aggagggcct caaggtgaag gccttggcct gtgcatccac gggagatacg
    16321 agtgcctcgc tggccgccta cggcgccgcc gcggggctgc ccgtggtcgt gctgctgccg
    16381 cgcggcaaga tctccacggc acagctggtt cagccgctcg cgcacggcgc gctcgtgctc
    16441 ggcttggaca ccgatttcga tggctgtatg accatcgtgc agcgcctcgc cgaagagggg
    16501 ctggtttatc tcgcgaactc gatgaatccg ctgcgtctcg aggggcagaa gacggtgggc
    16561 atcgaaatca cgcagcagtt cgactggcag gtgcccgatt ggatcatctt gccgagtggc
    16621 aacctgggta atgcgtccgc gttgtacgcc ggattcaaga tgatgcgaga actcggggtc
    16681 atctcgcgct atccgcggtt ggtggtggca caggccgaga acgcgaatcc gctctatcgc
    16741 gcttggtcgg agggcaagcg ccaagtgaca ccgatccaag ccaagcccac gctggccacc
    16801 gccattcaaa tcggcaaccc agtgagcgcg ccgcgcgcca ttcaagcgtt ggagtcgatg
    16861 aacggtctgg tggagcaggc cagtgaagac gagttgtgcg aggcgtccgc gcgcgccgat
    16921 cgtaccggca tgtactgctg cccgcacacg gcggtaggtc tcgcggtgct agagaagctc
    16981 gtggcccgca aggtcatcca gccgcaggaa aaggtggtct gcgtctcgac cgccgccggg
    17041 ctcaagttca cggagttcaa ggttcgctat cacgatcgcc agatcgaggg cgtacgcggc
    17101 gaacagtcga acccggccgt gtttctgccc gcagatttcg gccaagtggt ggacgccatt
    17161 caccgacgct tccagtaaat cggcatcgcc gtcgcgctgc ggcgctgaag ctttcgagtg
    17221 caggcgcgga gggcaggaga cgatt
//
LOCUS       DASTKU010000775         4489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_152607, whole genome shotgun sequence.
ACCESSION   DASTKU010000775 DASTKU010000000
VERSION     DASTKU010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152607"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFQ61_27065"
     CDS             complement(<1..903)
                     /locus_tag="VFQ61_27065"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET9958197.1"
                     /translation="MAERTAASELSASELPASLVRLLQASAPDARLVSARALGADEEL
                     VDSSHKGAGYGAPLLLELETSSGTARWVLHTATANPFGHDRRADRAAEMLLAYDTFGE
                     IPRHARVIDVGAYRRGGGSHSLADSGEFYLLTEWLSGSPYAHDLRVIGERALQPGSES
                     LTPSERRRAQNLARYLSALHSSKLTVANPETTYRRAVRDLIGSGEGLFGIRDGYPEST
                     PAASPERLDAIERRVLEYRSKLRARTARLARIHGDFHPFNILFDDEDEPRLLDASRGC
                     AGDPADDVVCLTVNYLFFGLLFEPA"
     gene            1067..3619
                     /locus_tag="VFQ61_27070"
     CDS             1067..3619
                     /locus_tag="VFQ61_27070"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET9958198.1"
                     /translation="MRPEAVRDSASHLEISDERGLSSAQAAERLRVEGPNALPEPPAR
                     PMWRRIFAQLQGALSVLLLSAVGLDFVLWIVHGAVGVPVEPIAILAVLLLNAGLGVLQ
                     EYRSEQALAELKSLSLPLVWVERDGELVHLPADHIVPGDWLRVEAGDRVAADASLVHA
                     NSLSIDESLLTGESLPIEKQAGEELFSGTLVARGTGFARVTRTGPKSTLGRLAAELSK
                     IDVGRTPLERRLDRFGRQIAIVVGSLAALIVLVGAINEGIGRIGAIITFAVAFAVAVV
                     PEGMPAVLTLALAIGVQRMVRRRAVVRRLAAVEALGSVTLIATDKTGTLTLNQMNVAE
                     VHSAVPERALEAAILANEAEPGSDAGDPLDVALLNYAASHGVDVARVRALHPKISGRP
                     FDSAWKFARHTAETPEGQVSFLKGAVETLNARSRSSPEQVAEFKAWNAAQAAQGRRVI
                     AVARAAGEDEHDLEILGLISVWDPPRPNVREAMLACMSASVRVVMITGDHPETARSIA
                     SLVGMRDPLVITGEQLRSASAEQQQTWIASVDVVARATPEDKLFLVGAFQRSGEVVAM
                     TGDGVNDAPALKKADVGVAMGERGSDVAREVSDLVLQDDNFATIVHAIEEGRNIYENI
                     QRFIRFTFSTNVALSILVLGGAVGAYVFRVRDGLGALAIPLTAIQILFINFVGDGPPA
                     LALALDRSPDVMRQKPRLPNSGLLDGPALKFIAMVGVLQGGLGLLLLVLIPTWGFGLH
                     HTQTAVFLYEAVAKLVSAYPARRVSGASVKNPVLHGCVAFGAGLALLCVYLPPVAHVL
                     GLVALPRELLLIVTISLSITWLLTQILVLMGRWSGRATPAPPGSSTQNLRHS"
     gene            3797..4471
                     /locus_tag="VFQ61_27075"
     CDS             3797..4471
                     /locus_tag="VFQ61_27075"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9958199.1"
                     /translation="MQHSKPYLIVVGVDYSKTGDLALERAFELATERENAEVHIVYVL
                     QTLSALAPVELGVGIGGPDPSLVKEASEQLRKYAERKLDEFKSKFAGTGKKRLFERAV
                     SHLRLDAPAQEVAQLAADLEADLVVVGTHGRKGATRLLVGSVAEGVVRLAPCPVLVVR
                     EKRVHEEGPKIEPPCPDCLEIRRTSNNKELWCARHREHHIIAHTYHYRDRVGEETNFP
                     MVTPER"
BASE COUNT          799 a         1289 c         1513 g          888 t
ORIGIN      
        1 tgcgggctcg aagagcagcc cgaaaaacag atagttcacg gtcaagcaaa ccacgtcatc
       61 tgcgggatcc ccggcgcaac cgcggctggc atcgagcagg cgaggctcgt cttcgtcgtc
      121 gaataggatg ttgaaggggt ggaaatcgcc atggatgcgg gccaagcggg ctgtccgcgc
      181 gcggagcttg ctccgatact cgagcactcg tcgctcgatg gcgtcgaggc gctcggggga
      241 agccgcgggc gtggactccg ggtagccatc ccggattcca aacaagcctt caccgctgcc
      301 gatcaagtcg cgcaccgccc gccggtaagt ggtttccggg ttcgcgaccg tcagtttgct
      361 cgaatgcaga gccgacaaat aacgggcgag attctgcgcg cgtcttcgtt cgctcggggt
      421 gagtgattcg gaaccgggct gaagtgcgcg ttccccgatg actcgcagat cgtgcgcata
      481 ggggctgccc gagagccact cggtcaacag atagaattct ccgctgtcgg ccaatgaatg
      541 actgccgccg cctcgccgat aggctccaac atcgatgacg cgggcgtgtc gcgggatctc
      601 accgaacgtg tcgtaggcca atagcatctc cgcggctcgg tcggcgcggc gatcatgtcc
      661 gaaaggattt gccgtcgccg tgtggaggac ccaacgggcg gtgccgctgg aggtttcgag
      721 ttcgagcagc aggggtgcgc cgtaacctgc acccttgtgt gaactgtcga cgagctcttc
      781 gtcggcacct agcgcacgtg cggacacgag gcgcgcatct ggcgcactgg cctggagcaa
      841 gcgaacgagc gaagcgggca gttctgaagc agacagttcc gaagcagcag ttcgttcagc
      901 catgcggcaa ccattttgga ctatttcgcg ggctcggcga actggtgagc aatcgccgct
      961 aggctttcgc acctcggatg tcgcatgctg ggcagtgcca atgacagggg attcgatacc
     1021 acccaagcgc gaggactcgg cgcaccccgc cgcacgatcg gaaaacatgc ggccggaggc
     1081 cgtccgggat tctgcttctc acctcgaaat ctcggacgaa cgcgggctct cgtcggcaca
     1141 ggcggcggag cgtttgcggg tcgaggggcc caacgctctg ccggaaccgc cagcgcgccc
     1201 gatgtggcgg cggatcttcg cgcagctcca gggagcattg tccgtattgc tgctcagtgc
     1261 cgtgggtttg gatttcgtgc tgtggatcgt ccacggtgcg gttggggttc cggtcgagcc
     1321 gatcgccatc ttggccgtgt tattgctgaa tgcggggctg ggggtactgc aagagtatcg
     1381 ctcggagcag gcgctggccg agctgaagtc attgagtttg cctctggttt gggtcgagcg
     1441 agacggtgag ctcgttcacc tgccggcgga ccacatcgtt ccgggagatt ggctgcgcgt
     1501 agaagcgggc gatcgggtcg cggcggatgc gagtctcgtg cacgccaact cgctctccat
     1561 cgatgagtca ctcttgaccg gggaatcgct gccgatcgag aagcaggctg gagaagagtt
     1621 gttcagcggg accctggtgg cgcgcggaac cggcttcgcg agggtcacgc gtaccgggcc
     1681 gaagagcacg ctggggcgct tggcggccga gctctccaag atcgacgtgg gccgcacgcc
     1741 tctcgagcgc cgactcgacc gctttggtcg gcaaatcgcg atcgtggtgg gtagtcttgc
     1801 cgccttgatc gtgctggtcg gtgcgatcaa cgaagggata gggcgcatcg gcgccatcat
     1861 cacgtttgcg gtcgctttcg cggtggcggt ggtgcccgaa ggcatgccgg cggttctgac
     1921 attggccctg gccatcggcg tacagcgcat ggtgaggcgc cgcgcggtcg tgcggcgcct
     1981 ggcggccgtc gaggcgctcg gctcggtgac tctgattgcg accgacaaaa cgggcaccct
     2041 gacgttgaac caaatgaacg tcgcggaagt ccactccgcg gtccccgagc gcgccctcga
     2101 agctgcgatt ctggccaacg aagccgagcc cggtagcgac gccggggatc cgctcgatgt
     2161 cgcgctactg aactatgcgg cgagtcacgg cgtggatgtg gcgcgcgttc gcgcactgca
     2221 cccgaagatc tccgggcggc ccttcgacag cgcctggaag tttgcgcgac acaccgccga
     2281 gacgccggaa gggcaagtta gcttcttgaa aggggcggtc gagaccctga acgcaaggag
     2341 ccgctcgagc ccggagcagg ttgcggagtt caaggcctgg aatgcggcgc aggccgcgca
     2401 gggccggcgt gtaatcgccg tcgcgcgcgc ggccggtgaa gacgagcacg acctcgaaat
     2461 actcggcttg atttcggttt gggatccgcc acgcccgaat gtccgagaag cgatgctcgc
     2521 ctgcatgtct gcctcggttc gtgtggtcat gatcacgggg gatcatcccg aaaccgcgcg
     2581 ctcgatcgcg agcttggtgg gtatgcgcga tccactcgtg atcaccggcg aacagttgcg
     2641 ttcagcgagc gccgagcaac agcaaacttg gatcgcgagc gtggatgtgg tcgcgcgcgc
     2701 tacgcccgaa gataagttgt tcttggtcgg cgcctttcaa cgcagcgggg aggtcgtggc
     2761 gatgaccggg gatggcgtca acgacgcccc cgcgctgaag aaggcggacg ttggcgtggc
     2821 catgggtgaa cgcgggagcg atgtggcgcg cgaggtctct gatctggtgc tgcaggacga
     2881 taatttcgcg acgatcgtcc acgccatcga ggaaggccgc aatatctacg agaacatcca
     2941 gcgcttcatc cgcttcacct tttccacgaa tgtggcgctg agcattctcg tcttgggcgg
     3001 cgcggtcggg gcttacgtat tccgcgtgcg ggatggactg ggagcgctgg ctataccgct
     3061 tacggcgatt cagatccttt tcatcaattt cgtaggagac ggtccgcctg cgctcgcgct
     3121 cgcactcgat cgcagcccgg acgtcatgcg tcagaagccc cgactgccca acagcgggct
     3181 attggacggg cccgccctca agttcattgc catggtgggc gtgctccagg ggggattggg
     3241 cctgctgtta ttggtattga ttccgacttg ggggtttgga ctgcatcaca ctcaaacagc
     3301 cgtctttctt tatgaggcgg tcgcaaaact cgtgtccgcc tatcctgccc ggagggtcag
     3361 tggtgcgtcc gtgaaaaatc ccgttctgca cggctgtgtg gcatttgggg cgggattggc
     3421 gctgctttgc gtttacctac cgcctgtcgc acatgtactc gggctcgtcg cactgccgcg
     3481 agagctgttg ttgattgtta cgatttcact gtccatcacc tggctgctga ctcagatttt
     3541 ggtgctgatg gggcgctggt ctggccgcgc aacgcctgca ccgcccggca gttctacgca
     3601 gaacctgcgg cactcgtgag ctgctgccga tgtaggtgag ggcttatgta tgctgattga
     3661 ctgcttcgat tctgctcacg agtcgacctg gtttgggcat actcgtgttc cactcagttg
     3721 gtgactccgg caggccgtgt gcgatggcac gccgaatgca ctttgaactc tcacatacac
     3781 ttctggagaa ggtgccatgc aacattcaaa gccatacctg atcgtggtgg gcgtcgacta
     3841 ctcgaagacc ggtgacctgg ccctcgagcg tgcattcgag cttgcaaccg aaagggagaa
     3901 tgccgaagtg cacatcgttt atgtgctgca gacgctgagt gcactagctc cagtcgagtt
     3961 aggcgtagga atcgggggac cggatccgag cttggtgaag gaggcttcgg agcaactgcg
     4021 aaagtacgcc gaacgcaagc tcgacgagtt caagagcaaa tttgccggaa ccggcaagaa
     4081 gcgccttttc gagcgcgccg tttcgcactt acggctggat gcaccggctc aggaagttgc
     4141 gcagctggct gcggatctcg aggcggatct cgtggtggtt ggtacccatg ggcgcaaggg
     4201 cgcgactcga ttgctcgtgg gctcggttgc agaaggtgtc gtgcgccttg cgccttgccc
     4261 cgtcttggta gtgcgtgaaa aacgtgtgca cgaagaggga cccaagatcg agccgccttg
     4321 ccccgactgt ctagagatcc ggcgcacgag taacaataag gagctgtggt gcgcgcggca
     4381 tcgtgaacat cacatcatcg cgcatacgta tcactaccga gatagggtcg gggaagagac
     4441 gaatttcccg atggtcacgc cagagcgtta aggggctgtg tttgttggg
//
LOCUS       DASTKU010000776         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_374890, whole genome shotgun sequence.
ACCESSION   DASTKU010000776 DASTKU010000000
VERSION     DASTKU010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374890"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..148)
                     /locus_tag="VFQ61_27080"
     tRNA            complement(75..148)
                     /locus_tag="VFQ61_27080"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Ile,seq:cat)
     gene            597..>2335
                     /locus_tag="VFQ61_27085"
     CDS             597..>2335
                     /locus_tag="VFQ61_27085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9958200.1"
                     /translation="MQKLVVRGASQHNLKNVSCELPRNRLVVITGPSGSGKSSLAFDT
                     IYAEGQRRYVESLSAYARQFLDQLPKPKVESIEGLSPAIAIEQKGLGKSPRSTVGTVT
                     EIADYLRLMYARVGVPHCPKSGKVLRAYTVQEIVDSIASRGDGAKIALLAPILRRAKG
                     NVAAELERLRRDGFVRARLDGRQVDLGDEMTLDPDAEHELDVVVDRIVVREGAKGRIT
                     DSVELSLKLGDGTLLIDAMDGSEPLVMSERLVSWEYGITLPPLEPRLFSFNSPHGACP
                     ACDGLGVRSGIDVARVVPDDSRSLREGAIAAFGRRGSVATATEVAHVVEALGVDPDVA
                     FRDLSEEARRAILFGTEAAPLKRRGRAREYVGVVPRLTRMLETQEAPEAEDELEDGAI
                     GPEDLGRFAVTQTCETCNGLRLRPEALAVKLASKHIGEVCRLPLRSLKVFLEEYSQSD
                     ELLGRDATIALPLVRAVTERLGFLIEVGLDYLTLDRAAHTLSGGEGQRIRLATQIGAS
                     LVGVLYVLDEPSIGLHARDNERLLSALKRLVEKDNSVIVVEHDRDAILAADYVVDMGP
                     AAGVHGGEIIAEGS"
BASE COUNT          415 a          728 c          795 g          397 t
ORIGIN      
        1 ggggcttgcc gcgctgtatc atgccgcgct gtatggtctg gcggcgctgt atgctctcgc
       61 ggcgctgtat gcggtgggcc cacctgggtt cgaaccagga acaacccggt tatgagccgg
      121 gggctctgac cgattgagct atgggcccgg tctctagcca tttgcgccgc cgtattcgcc
      181 aatctgtaag cctgctggct cacggagcca gcgccggtcc cggcgttcag gtcggttccg
      241 gcgtcggcgg tcaggcgttc gcgctgcacg gcgccgaaaa gtagtcgaac gtgctcggcc
      301 ccgcaacagg atgggcaccc gaattcgctc gagtttcagc ggagctccga cgcggcgctt
      361 gccgcacgtg accgtgatcg gttttgcagg ccgcgcgccc cgaaccccga cggcgcctaa
      421 acccggtcta aggcagggct cgaccgcccg gaaaataacg gtcctgatgg aggcgaacag
      481 acacggtctg gcgggccgga gccaattcga aggtgtgcga gcccgcaacc ttcggtaaac
      541 cgtggtagat gcgcggcggc gctcgaatcg atacgccgcc gcccaagccc aagcacatgc
      601 agaagctcgt tgttcgaggc gcgtcgcagc acaacctaaa gaacgtgagc tgtgagctcc
      661 cgcgcaatcg cctggtcgtc atcacgggac cgagcggctc tgggaagtca tccctggcgt
      721 tcgacacgat ctacgccgaa gggcagcgcc gctacgtcga gtctctgtcc gcgtacgcgc
      781 ggcagtttct cgaccaactg cccaaaccga aagtggaaag catcgagggg ctgagccccg
      841 cgattgccat agagcagaag ggcctcggaa agagcccgcg ctccacggtc ggcaccgtca
      901 cggagatcgc cgactaccta cggctgatgt acgcccgagt cggggtgccg cattgcccaa
      961 agtccggcaa agtgctgcgc gcgtacacgg tccaggagat cgtggacagc atcgcaagtc
     1021 gcggcgacgg ggcgaagatc gcacttctcg cgccgatttt gcgacgcgca aagggcaacg
     1081 tcgccgcgga gctcgagagg ctgcgccggg acggcttcgt ccgagcccgc ttggatggtc
     1141 gccaagtcga cttgggcgac gagatgacgc tggacccgga cgccgagcac gagctggatg
     1201 tcgtggtgga ccgcatcgtg gttcgcgagg gagccaaggg ccgaatcacg gactcggtgg
     1261 agctctcgct caagctgggc gacggaacgc tcctgatcga cgccatggac ggcagcgagc
     1321 cgttagtgat gagcgagcgg ctcgtgagtt gggaatacgg gatcaccctt ccacctctgg
     1381 aaccgcgcct gttctctttc aatagcccgc acggcgcctg tccggcgtgt gatggcttgg
     1441 gggtgcgatc gggtatcgac gtggcgcgag tggtgcccga tgactcacgc tccctgcgcg
     1501 agggagcaat cgccgcattt ggccgccgcg gctcggtcgc gactgccacc gaggtcgccc
     1561 acgtcgtcga ggcgctcgga gtcgaccccg atgtggcctt tcgggatctg tctgaagaag
     1621 cacggcgcgc catcctattc gggaccgagg ccgcccctct caagcgacgt ggtcgagctc
     1681 gagaatatgt cggagtggtg ccgcgcctga cgcgcatgct cgagacccag gaggccccag
     1741 aggccgaaga cgagctcgaa gacggcgcca tcgggcccga agacttgggt cgctttgcgg
     1801 tgactcagac ctgcgaaacc tgcaatgggt tgcggctgcg tcccgaggcc ctggccgtga
     1861 agctggcgag caaacacatt ggcgaggtct gccggctccc gctgcgaagc ctcaaggttt
     1921 tcctcgagga gtactcgcag agtgatgagc tgctcggccg cgacgccacg attgctttgc
     1981 ccctggtacg cgcggtgacg gaacgactcg gcttcttgat cgaggtcggg ctcgattact
     2041 tgacgctgga tcgggccgcc cacacccttt ccggtggtga ggggcaacgc attcgactcg
     2101 cgacccagat cggggcctcg ctcgtgggag ttctgtatgt actggacgag ccgagcatcg
     2161 gtctgcacgc gcgagacaac gagcggctac tctcggccct caagcgcttg gtagagaagg
     2221 acaacagcgt catcgtggtg gaacacgatc gggacgcgat cttggcggcc gactatgtgg
     2281 tcgatatggg cccggccgcg ggcgtacacg gcggagaaat catcgccgaa ggctc
//
LOCUS       DASTKU010000777        27001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_599345, whole genome shotgun sequence.
ACCESSION   DASTKU010000777 DASTKU010000000
VERSION     DASTKU010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 27001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27001
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599345"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1633)
                     /locus_tag="VFQ61_27090"
     CDS             complement(182..1633)
                     /locus_tag="VFQ61_27090"
                     /inference="COORDINATES: protein motif:HMM:NF027224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HET9958201.1"
                     /translation="MKFGARLVPALLSLTATAYGAAPGITLLRDTTLSANATAVKSYD
                     TNVNIASYRHQGILYYSGYQFVAWYNGNARNIIVARRSFNQDSLAAGTWAWAYVNFNL
                     AAGADSHNVISLAISPNDARLHVAIGQHDAQLYSIRTNSNAITGAIWDNTVFGGTASA
                     PATPMAGLPGYPAATNGITYPYFMNAGDPSRTLQFAYRTGSSGNGVLHLAEYNPSTSS
                     WSYLGQFTAAAGSYTQNGVTSTSRNAYPHGFNYDYGGRLHMAFTWRENLSTQFIPNCG
                     GSTINNHDTLYVYSDDRGRTWRNSAGTAVAKTGTPDLIALSDSGLVVDPLPVGRDLMN
                     QETQIMDRNNLFHAFVSYVPGRYQATCATDRSVAQPFHLWRSPSGVWTKVEMKLGGAD
                     VNQGYDRSKMFFGTANNLFVVLPDLRIVGASAASNWTDWKLIYDGRGLGNYGETIIDY
                     NLTRVANAVSVVYIKNGSGTTAELHVLDFKTTG"
     gene            complement(1808..3763)
                     /locus_tag="VFQ61_27095"
     CDS             complement(1808..3763)
                     /locus_tag="VFQ61_27095"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9958202.1"
                     /translation="MKRQISEGSKTHDGQGRFALRRIQRSFPAVLALCLASSVAHAGA
                     EDPERNATEKVNANWVTGFSGESLEQRRVAGERIVDLNIESTAPWRFTAAFVANTGTY
                     QRVDRFWFDKTAAQVTSALSENNARIVSIDASYVNGQPRFAFSTVRNEGTAAKGWWWN
                     YDVTVDQVTRDINLHHLQLTSLKSYDTPNGRRFVYVGIKNEGTDARNWWWHPGETPDG
                     IGRMLRDNSARLVTMDRTEPGRFAVIMVQDPSPRYSWWAHGTEQERTREIQATHGVRV
                     AAVDSYVVGGKRQYSFVGIDNTNAETARLRGYLDQAYDDTTQFGSDVIRGAYVKQVGG
                     PVLVDLAANLRFQPLSTLKLLPYLYVWDRIDNATKEADNAPTTLATRISWTEATGDPF
                     ASCLTPGAAGTRQNSAELRDALPTMMWYSHNRTLDSILERYDPDTITTRGRALGLLDT
                     EMHFGCPQPGDPVPWAKNRSTLIDIGRIFEGVHQGTMLRNNKSSRDGFFDNIIRLNYA
                     GAEYLSPYLPQKVGPLFNGFLRDIVRREAGPNKQGQVEEFLKRVVLRGKGGSGGPSSD
                     EFGYSDALYVELPVWKNGAVTSSYYVSTWFIYKLHAPAGCPDSDPPAGSGCDRIWQPE
                     QDVHETFKREILAGPIRAALATWPASP"
     gene            4423..5295
                     /locus_tag="VFQ61_27100"
     CDS             4423..5295
                     /locus_tag="VFQ61_27100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958203.1"
                     /translation="MPSLLHEGIIELIRDRPEVAPKLLSELLHVEVPQFSEARLAEAN
                     LTQLLPVEYRADAVVLLVDGKPVFGIVIEAQLQPDERKFFSWPVYAVTARARYECPCV
                     VIVITPESDLADWASKWIDLGGGMQWRPLVVGPEGIPVITDPQQASRVPELAVLSAMA
                     HGRGSPEVAVAIALAAVEGMAGLPEEPRVLYCAFIESALSDAARKAIQMPLAEKFFSE
                     TQRHAFARGKAEGKTEGKAEGQAQFVIRVLVNRGLSISEAQRERILTCHDTEQLTRWL
                     DAALSCGSVDELLH"
     gene            complement(5319..6215)
                     /locus_tag="VFQ61_27105"
     CDS             complement(5319..6215)
                     /locus_tag="VFQ61_27105"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9958204.1"
                     /translation="MSNVSAKGPISPPSPGTIVTRDGETLYCRDVGHGPPVVLVHAWG
                     LSHRAWQYQTGALQDAGLRTLSYDRRGHGRSSPAASGYDYDTAADDLDAVLDQRGVQR
                     AVLVGHSQGCADIIRYLSRYGSARVRSVVLIAPLAPILLQNEDNTEGLPLAAFEQLWA
                     SWRHDFAEWVEAGKRPFFMPDTPLPIMDWVAGLLLETHLDVLLATSRTAAHSDLLPDL
                     QAVDVPCLVIHGDRDVSAPLALGQRTAEGIRDARLAIYQNAPHGLLFTHTERLNRELI
                     EFAHLSDDRKTVGRANGDPPMR"
     gene            6714..7022
                     /locus_tag="VFQ61_27110"
     CDS             6714..7022
                     /locus_tag="VFQ61_27110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958205.1"
                     /translation="MRAKLASDADADEPQIEVSECYDVRVVELAWLSSRMLWIWGGEK
                     LAPNPRRREGLVAASYRCERHYLMAMRLLGLRSMRGAAAELERAYEIENQFLTFLGAA
                     "
     gene            7047..7976
                     /locus_tag="VFQ61_27115"
     CDS             7047..7976
                     /locus_tag="VFQ61_27115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958206.1"
                     /translation="MNYCTSRRESRVTAYVVLLVAAGAACSRGSSVRGDGLGGTGGAS
                     CSTPDAGQGSRHSGGRETEFSASGSSGELAADGGAKALISDSSAGEANPETNSAGEDN
                     GAGNGGARGDVDNEVGGSAGASGGGAGAAGTPTEPESRPWKLVFITPDAFPSDFGRDG
                     SERGVGAHVRADALCQQAAIDAGLRGQFRAWLSTSTSAAINYFAILDMNGPWYRADDE
                     LVAEDLSALVSKNGLRAPILYTPNKVPGGMFWTGTGPLGTLARNCDDFTNANLNDGLA
                     GHCALATGQEYGWPNTAFIACGSTNRALACFER"
     gene            complement(7996..9939)
                     /locus_tag="VFQ61_27120"
     CDS             complement(7996..9939)
                     /locus_tag="VFQ61_27120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958207.1"
                     /translation="MNLPAVESLVEALFSKLSAERALVEYFRQRIERLCEAVSAHDRA
                     RHRETELALRCALASVDVVGLGALNGHRSSAVAGQLTAVATDIQHQLDALEAELGKPL
                     AERTLTNVLVGLSRSSAQLARLELTLNHAPSRLKLWLAPLCALPAIGVYLIGMSLRVG
                     PTGLSEREQALTLVGSGFPAAEAPNVLAPEVRRAAFKTFSRAYFSGDLRAQKRMPLRY
                     VLSQVLSNRSHADSLMLSAVSVDVRFHSAPFPWKELPTEPQLQVSWTRGKFELADEPF
                     SVMLPAPSITNVGDGPAVGFHCEFQTATKQLPISDLNDTLLLLDSPRAWDLDVVDASV
                     HGRNIRGGELSPPVYVATDVGSDDDLVCGRTRYRKTSNPDDLCRATECARNEPFKLTC
                     EYQSLRTPEVTRVTYEAPPDPSVILFRATDRVRLRPSCSLTGGSSIKVREPWQRALEV
                     AEAAISEPPRVPTPEGEPLIVKQLELDVANLESGGVLGFREPVHRFLRAKGLLALELS
                     LTRPRNGSYTVTVHVGADQVAHRSIEVLVPEDLNVHEVPLSTLFRRFGRPELLHLAAL
                     ESRSSGESDADPRAGATPPPSASSGDQHVTSTPADREPSRSAPPRGDSAPRRITVSAA
                     LPEDLAVSEAVAEPNAPAEGPAE"
     gene            10134..10418
                     /locus_tag="VFQ61_27125"
     CDS             10134..10418
                     /locus_tag="VFQ61_27125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958208.1"
                     /translation="MIERQFNGRGTTERMLGAQVFVPAQPGLTAEWLRSRVEQHVSGM
                     RHTGMAGCPLGVSNLRVALVSGGTGCAKKNSGEGARGGGLYRIWILNRRC"
     gene            complement(10761..11603)
                     /locus_tag="VFQ61_27130"
     CDS             complement(10761..11603)
                     /locus_tag="VFQ61_27130"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9958209.1"
                     /translation="MPEKTSPNTGMATTTLPAFLRDRRERLQPTTTVRRRTPGLRREE
                     VAARAGVSVTWYTWLEQGRGGMPSDDVLERLSDALELDATNREILFLLAHGRPPPLKP
                     TKPAAVTPSLQRVLDHLSVPAFIKTPTFQVVAWNRAAVAVLGDYSQVPERDRNLLRRV
                     FQPGSENKVQHVEVVRRACVASFRVDIARAGTSEEAAALIDELSETSPEFRRLWREGE
                     MRPHSEFRKRLIRPDVGEIELEASVFYVEGSDGLSMFVWSPVNEASAIALEQLVERSA
                     ISRR"
     gene            11628..12530
                     /locus_tag="VFQ61_27135"
     CDS             11628..12530
                     /locus_tag="VFQ61_27135"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9958210.1"
                     /translation="MTKTMRVFVTGATGWIGTAVLKELRAAGHRVLGMAHTDAGAEKL
                     SAQGVEIYRADLREPTSLVEGVRKTDATIHLGFHMDFSDLSKMYELDRQAIRTMLDAM
                     AGSNKTFLGTNGTLVVAHPGKLVSESDTEPTNSVLSVRLSAERLVLDAVKSGVRGSVI
                     RLAPTVHGSGDKGFIPMLIQLAREKRAAAYVDEGGNHWPAVHRDDAACLYRLALENAP
                     PGTVLHATAEEGIRMREIAETISQGLGVPTKSVPAATAADHFGWLSIAVGLDNRSSSA
                     ATRQLLRWAPQGPGLLQDMRAHYF"
     gene            complement(12568..13116)
                     /locus_tag="VFQ61_27140"
     CDS             complement(12568..13116)
                     /locus_tag="VFQ61_27140"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HET9958211.1"
                     /translation="MSQLARGVAANAENARFIASPLPVVLHVLGATLFCLLGALQFAP
                     FTRGSRWHRVVGRVVFPAGVVAALSGLWMGLVYPLPLADAHPLLKGFRVVLGLGMVLT
                     LVLGWRAILRRDIRAHRAWMVRSYAIGIGAGTQALIMVPWWLLFGKPAGIAYALLMGA
                     GWALNLVVAEYRVRVPHRRPTA"
     gene            13334..14062
                     /locus_tag="VFQ61_27145"
     CDS             13334..14062
                     /locus_tag="VFQ61_27145"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET9958212.1"
                     /translation="MATAAGDELAQSAVRIEEEILVSEGRMLGRDAELRGRLRISTVD
                     FIFAGFPDVFESFIRRYPGVDLTVGVSNERVSLIRREADVALRLVNSPGERLVGRRLG
                     RLQFEAYAARALVERLGRHAPLSDFPWIHWDERSDGTWLDAWLGKHAPGARISLRSDD
                     YAVRRRAISSGIGVHFLACFDGDADPALVRLGARLSDEARDLWVLTLPELRANSRIRA
                     FMEHAHSCFKRHRRALEGAATRGT"
     gene            14532..14699
                     /locus_tag="VFQ61_27150"
     CDS             14532..14699
                     /locus_tag="VFQ61_27150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958213.1"
                     /translation="MTRWSFAEGRRRLEVRSQLAVLEECELLVCSSFPRKRSCPSGKL
                     VVAILEVVCAF"
     gene            15303..15863
                     /locus_tag="VFQ61_27155"
     CDS             15303..15863
                     /locus_tag="VFQ61_27155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9958214.1"
                     /translation="MADPARKRASYSDLHSVPDHLVAEIVDGELVTSPRPAPRHARAA
                     SSLGGELYGPFDRGRGGPGGWSILDEPELHLSGDALVSDLGGWRRERMPLLPEVSAFE
                     LAPDWVCEVLSPSTAPLDRTRKLPIYARERVQHVWLVDPSLRTLEVLRLDGGGYRIVG
                     AWHGDAIVCAEPFEAYALHLADLWSA"
     gene            complement(15992..16630)
                     /locus_tag="VFQ61_27160"
     CDS             complement(15992..16630)
                     /locus_tag="VFQ61_27160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958215.1"
                     /translation="MASGKGWYGEPVAEAIKGLRACHGLVLYPGRYNFYYLPRSKLVL
                     SAEELEPKDVMLQRHRDELLGALQLTHEDLEANRRGQLTRRQADSWRTDVWVTVLFAL
                     LLASLAGAAWFFSGHPSATLIFGLWAAIIGYRSLISSRRHRVLAFRGQLLSKDIGPEE
                     RSHLCFSGHGIQKLFQLEWSINERVTTLVIVGANYAVYYTGGKVAVAVEPVE"
     gene            complement(16993..17394)
                     /locus_tag="VFQ61_27165"
     CDS             complement(16993..17394)
                     /locus_tag="VFQ61_27165"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HET9958216.1"
                     /translation="MVTIHVVGAAIFQSGRCLVARRGPSMTLPDKWEFPGGKVEPGES
                     AATALAREVAEELGLEIAVKRRLATGTAPVGSRLISLEVFAATIISGTLVLREHSQVI
                     WATVEDLAHLDWAAADIPCVPHVQAWLRTES"
     gene            complement(17701..18426)
                     /locus_tag="VFQ61_27170"
     CDS             complement(17701..18426)
                     /locus_tag="VFQ61_27170"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9958217.1"
                     /translation="MHTAGRRGATRVVSFGLMRTPAPSLEDSTVYPVEEPVGEDILQC
                     WMLEELRPLIERWLAERGTPWLVGSDQFIYFEKGQVSKRIAPDIYVLPGVPPNTRIPS
                     WKVWESGIRPSFALEICSQDWQKDYVTAPQLHNEVGTEELIVVDPDHSKRREGHRFQR
                     FERTSQGLELRERTQADRVRSRVLGCFIRLVGTEQEPRQHVRLGLDPDGEVLFPTQEE
                     AERQRTEAALARVAELEKLLERR"
     gene            18949..20319
                     /locus_tag="VFQ61_27175"
     CDS             18949..20319
                     /locus_tag="VFQ61_27175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958218.1"
                     /translation="MFTELADAWDRLREEDEQLLDAQWSSWTRYALQHSDDPHWYSSI
                     RNLAVYSGVGLSYALWKASTSSATGFIDVLRLGDGIRQGGWGYGRDALRLLVLTGPAL
                     RLARLGLTKLASLDTFEANANCAWVAAAAALRMTGIKHFATLGDLARAAEMPVAGLGE
                     VKDLSVVAPFIQRLGGVTRFIGTPSAEAEQTIARTVAANPNSAVLFGLRWDVGGQQVG
                     HAMAAFRVLGRTMMLDRDGSIYQNLAQIEALGGQATGISKAFVSELLIVENATIATAV
                     SRISQGAAIGNALASIRKVTPSASEHRVSASNSYLAKSGDLLPLIESIGLEVRSVPMR
                     SEGSLSSNSRKPRGASLSSMKHKITTSCIDQSNSIFLKEGGRAAIARRWCGNLTVDYK
                     VYVVRPGDFLARIAKIAYGDETKWPVLYRANRTKLGPNPHNPTALVPGMELEVPVSAS
                     QRIWVE"
     gene            complement(20967..22199)
                     /locus_tag="VFQ61_27180"
     CDS             complement(20967..22199)
                     /locus_tag="VFQ61_27180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958219.1"
                     /translation="MVLLLACSSVPAPGEAPGKGGSSSSSGGAASFGGSATTAGSTSA
                     GAASSSGGTATGSGGSTTTAGSTSASGGSTNSGGSSAGFGGSSAGFGGSSANSGGTSA
                     GSGGSSTNSGGSSAGLGGSSANSGGTSAGSGGSSANSGGTSAGSGGSGGQVNTTKVFL
                     AGDNVNANGPLQTTVQDLSFSGGGHILVRPTTLTSEPNTKHPVAIWGPGGGTPPRDYL
                     QLLTRMASQGFVIVGIRESTGDAVLMSAAIDWLEDEDANPSSPMHDRLILNRVGVFGH
                     SMGGLSSEAVAQTDKRVSTALLDNSGSFEHNALVNLKIPVGIIYGETGQEAPNAQGDF
                     NNVGNKGPVWLGMMKGGGHGSGPWDGASANVAWMRWHLGGENSRKEEFLSSGGDYNNT
                     GVWQAKQKNWEGWKNWNE"
     gene            23097..23276
                     /locus_tag="VFQ61_27185"
     CDS             23097..23276
                     /locus_tag="VFQ61_27185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958220.1"
                     /translation="MRAVGLLDGMVRAALGRHEKGELLACAVEVTNALGLSASDEPVA
                     GNYTDVRSRCHGRHG"
     gene            23505..23705
                     /locus_tag="VFQ61_27190"
     CDS             23505..23705
                     /locus_tag="VFQ61_27190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958221.1"
                     /translation="MAIRSEWQPCSIALRLGHCPTANELDELAIEPLGVSEEQSVRCV
                     LVDRESRGADADGGALAERQGR"
     gene            complement(24285..24494)
                     /locus_tag="VFQ61_27195"
     CDS             complement(24285..24494)
                     /locus_tag="VFQ61_27195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958222.1"
                     /translation="MASYNSIVAFAEQATPGDYLPEFDDTFAEWLGPVVSGWDPEIAE
                     CDRRLNQRGLAIEVDDRGLVARAWT"
     gene            25109..26878
                     /locus_tag="VFQ61_27200"
     CDS             25109..26878
                     /locus_tag="VFQ61_27200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958223.1"
                     /translation="MVAIAREYRVSRLIRWLWCGASLALAGCREQDSALSGDELGSSV
                     LLGTTVVESAAGLPACNLVRRGGVFYARAENALYYCDGRAFHALDLNGRPGEDGRGWA
                     VAVRDAAAECVSGGVVVSAGPDANGDGALDEVASTSAVCDGTAGASPPPLLRSRLLAP
                     GPECPGGGHWVEFGTDVNGNLSLDDDEVSSAEAFCEPQNLWRGDVVVDGDGELEALRG
                     FTEMIGNLTVTGDVTNLGPLESLTRVHGRLQINESRVLNLRGLAALEQVDGDLVVDGN
                     LSLLNLQGLASLAVVTGRFRVSQNWSLESLAGVESLQQVGNLYVESNNGVDTTALGNL
                     TRVDGDISLEGANMRGLRGMTRVNGSLSVSVAGVSDLEGLESLLEVGGSLYISGSSLL
                     NLKGLSRLKRVGGTLWVAAKHIQSLEGAESLTAIGGNLEIPSLSPEIMTVRGLDGLQT
                     IGGSLQLYSAIRDLSGFGSLESIGADAVLDGNALVNLHGLERLTNVGGSFVIQNAGLL
                     QSTAGAGALREIGRDLRIYNNGSIRDLRGFFGLERVAGSLIINFNPSLPACEAEWLRD
                     LVLGRRGVGADIAIFNNSQESCP"
BASE COUNT         5105 a         7928 c         8655 g         5313 t
ORIGIN      
        1 ccagcgagcc gtcgagggct cgtatcaacg gcttagggaa gttgagtacg agagagcgcg
       61 ccttgccgag gagaaggcgc gccttgccga agagaacgcg cgcctggccg aagagaaagc
      121 gcgcctcgcc gaagagaaac gacgttgggc cgacccattt tggtaggcta tcacctcggg
      181 cttacccggt ggtcttgaaa tccagaacgt ggagttcggc ggtcgtgccg gagccgttct
      241 tgatgtagac gacagatacg gcattggcta cgcgggtcaa attgtagtca atgatggttt
      301 cgccgtagtt ccccaaaccg cgaccgtcat aaatgagctt ccaatccgtc cagttgctgg
      361 cggcgctagc tccaacgatc cggagatccg ggaggacgac gaatagattg tttgcggttc
      421 cgaagaacat cttcgagcga tcgtagcctt gattgacgtc cgctccgccc agcttcattt
      481 ccactttggt ccagacgccc gaagggctgc gccagagatg aaacggttgc gccacactgc
      541 gatccgtagc gcacgtcgcc tgatagcgcc ccggaacgta gctcacgaat gcgtgaaaca
      601 ggttgttgcg atccatgatc tgagtctcct gattcatgag atcgcgaccg accggtagag
      661 ggtcgaccac tagaccgctg tctgacagcg cgatgaggtc tggtgttcct gtcttggcga
      721 cggccgtgcc tgcactattc ctccacgttc tccccctgtc gtcgctatag acgtagagcg
      781 tatcgtggtt gttgatggta gagccgccgc agttcggaat gaactgcgtg gacaggttct
      841 cgcgccacgt gaacgccatg tgcaggcggc cgccatagtc gtaattgaac ccgtgggggt
      901 aggcgttacg ggatgtactg gtcacgccat tctgagtgta gctgccggcc gccgccgtga
      961 actggcccag gtagctccag ctgctcgtgc ttgggttgta ctccgcaagg tgcagcacac
     1021 cgttcccgga actccccgta cggtacgcga actgaagcgt gcgcgatgga tcaccagcat
     1081 tcatgaaata gggataggtg atgccattcg tggcggcggg atagcccggg aggcccgcca
     1141 tgggtgttgc cggagcgctg gctgtgccgc cgaataccgt gttgtcccag atcgcacccg
     1201 tgatagcgtt gctgttggtg cggatgctgt aaagctgcgc atcgtgttga ccaatcgcta
     1261 cgtgaagccg cgcatcattc ggtgatatcg cgagcgatat cacattgtgg gagtcagctc
     1321 ccgccgctag gttgaagttg acgtacgccc acgcccaggt gccggccgcc agagagtcct
     1381 ggttgaagct gcgccgcgcc actatgatgt tacgcgcgtt gccgttgtac caggctacga
     1441 attgatagcc cgagtagtag agaatcccct ggtgacgata cgacgcaata ttgacgttgg
     1501 tgtcatagct cttgaccgcg gtggcgttcg cggacaacgt cgtgtcgcgt agcaaagtga
     1561 tgcccggggc cgcaccgtat gcggtggcag tgagggacag gagagcgggg acgagacgag
     1621 cgccaaactt catgggattt ggtgcaatct accacgctcc tgtgcgctcc tgcaggttca
     1681 ttccagccgc ttagaacggt tcttagctcg attggcccga gccaaacgcc cgggccaatc
     1741 tggattgtgc gtgcgggtcg cgagcgggaa tgactgcgcg acctaggctg ctttcgaaaa
     1801 agtaacccta gggtgatgcg ggccaggtag cgagagccgc gcggattggg ccagccagga
     1861 tttcccgttt gaacgtctca tgaacgtcct gttcgggctg ccaaatccga tcgcaaccac
     1921 tgcccgccgg cggatcgctg tcggggcaac cggcaggtgc atgtagcttg tagatgaacc
     1981 aggtgctgac atagtaagag gaggtgactg cgccgttttt ccaaactggc aattcgacgt
     2041 acaaagcatc cgaataaccg aactcgtcgc tgctcggtcc gccgctgcca cccttgccgc
     2101 gcaacaccac acgcttcagg aattcttcga cttgtccttg cttgttcggc cccgcctcgc
     2161 gacggacgat gtcccgcaaa aagccgttga agagtgggcc gaccttctgc ggaaggtacg
     2221 ggctaaggta ctcggcaccc gcgtagttca gacgtatgat attgtcgaag aatccgtctc
     2281 ggctactctt gttgtttcgt agcatcgtcc cctgatgcac cccctcgaag atccgcccga
     2341 tgtcgatcaa cgtgctgcga ttcttggccc agggcaccgg atcaccgggt tgcgggcaac
     2401 cgaagtgcat ctctgtgtcg agcaacccca gcgcacggcc ccgcgttgtt atcgtatctg
     2461 ggtcataacg ctcgagaatg gaatcgagcg tgcgattgtg cgaataccac atcatcgtcg
     2521 gcaacgcgtc ccgaagctcg gcggagttct ggcgcgttcc agccgccccg ggcgtcaggc
     2581 aactcgcgaa ggggtcgccc gtggcttccg tccagctgat gcgtgttgcc agcgtagtcg
     2641 gggcgttgtc tgcttctttg gtggcgttgt cgatacgatc ccaaacgtac agatagggca
     2701 acagcttgag cgtgctcagc ggctggaagc gcaaattcgc cgccaagtcg acgagcaccg
     2761 ggccacccac ctgcttcacg tacgcgccgc ggatgacatc gctgccgaac tgggtcgtat
     2821 cgtcatacgc ttgatcgaga tagccgcgta aacgtgcggt ttcggcgttg gtattgtcga
     2881 tgccgacgaa cgagtactgc cgcttgccgc ccaccacgta actatcgacg gcggccacgc
     2941 gcactccgtg cgtagcctga atttctcggg tgcgctcctg ttcggtaccg tgtgcccacc
     3001 acgagtagcg gggcgatgga tcctgcacca tgatcactgc aaatcggccg ggctccgtcc
     3061 ggtccatcgt gacgagccgc gctgagttgt ctctcagcat ccgcccgata ccatcggggg
     3121 tttcgcccgg atgccaccac caattgcggg catccgtacc ctcgttcttg atgccgacgt
     3181 acacgaagcg gcggccgttc ggcgtatcat aactcttcag actggttagt tgcaggtggt
     3241 gcaggttgat gtcgcgagtt acttggtcaa ccgtgacgtc gtagttccac caccagcctt
     3301 tggccgcggt tccttcattg cgaaccgtcg agaacgcgaa ccgcggctgg ccgttgacat
     3361 agctggcgtc aatcgacacg atgcgcgcgt tgttttcgct cagtgcactc gttacttgcg
     3421 ccgccgtctt gtcgaaccag aagcggtcca ctcgctggta ggttccggtg ttggcgacga
     3481 aggcggccgt aaaacgccag ggagccgtgc tttcgatatt caggtcgacg atgcgttctc
     3541 cggctacccg cctctgctcg agggattcac ccgagaaccc cgtcacccag ttcgcgttca
     3601 ccttctccgt cgcgttgcgc tccgggtctt ccgcgcccgc gtgagcgacc gagctggcca
     3661 agcagagcgc cagtaccgcg gggaaggagc gctgaattcg ccgcagagca aaccgccctt
     3721 gcccgtcgtg tgttttggaa ccttctgata tttgtctctt cattttgccc ctgtgattgg
     3781 cagatttgct ccttgcaaag ggagccgtgc cgcgttcgcc aatcgcaaac tcggccacta
     3841 tcgacgactt ggctcgagcg tcattgggtt tcgtacgcac aaagccatgc cctaccggcc
     3901 agtgaacacc gtgaatcact ggccttcacg ccgagtcgcg ccgcctccgg cgctcctcgt
     3961 gcggatgggc tctaagcagc tagcatgcca atccgtgtaa ccagtcttcg gccctttgga
     4021 attgcggaaa gccccactcg tggggactct tcatcagcgc gcgtgccagc cccgcgtgcc
     4081 aaatctggcc gctctgctgt gcccatgttg gtccatgcat cgacgacggg caggcaatta
     4141 cagccgagcg ggcggattct ctacccactc tccgtcacgg gcaaggcatt tgcgcggccg
     4201 caaaaccgag ctgcagccac gcccgctttg cttccgtcat gattgccgtt cgcgccgagt
     4261 gcctcgcttg gaaagcatgc tgcacggcgc tcggtcgagc tccaatgtca atcaactgag
     4321 cggacgctct gagaaatcgc gcacgggata tcccgagagc cgatgacaaa gtttgttgtg
     4381 aatgctcttg atcgctttct tccgaaatga catgtgaggg gggtgccatc actgcttcac
     4441 gaggggatca tcgagctgat tcgcgaccgg ccggaggttg cgccaaagct gttgagtgag
     4501 cttttgcatg tggaggttcc gcagttttcc gaggcgcgcc tggcagaggc gaatctaacg
     4561 cagttactgc cggtggagta ccgagctgac gcggtggttc tgctcgtcga cggcaaacct
     4621 gtgtttggca tcgtgataga ggctcagctg cagcccgacg agaggaagtt cttctcctgg
     4681 cccgtatacg cggtgacggc gcgcgcccga tatgagtgcc cgtgcgtggt gattgtgatc
     4741 actcccgagt ccgatcttgc ggattgggcc tcgaagtgga tcgacctcgg tgggggcatg
     4801 caatggcgac ccttggtggt cgggccggag ggaattccgg tcatcaccga tccgcaacag
     4861 gcgtcacgag tccccgaact tgcggtgctg tcggcgatgg cgcacggccg gggtagccca
     4921 gaagtcgcgg tggccatcgc gttggcggcc gtggagggta tggcgggcct tcccgaggag
     4981 ccgcgggtgc tatactgcgc cttcatcgaa tcagccctga gcgacgcagc aagaaaggcc
     5041 attcagatgc ccctagccga gaagtttttc agcgaaaccc agcgccacgc attcgccaga
     5101 ggcaaggcag agggtaaaac cgagggcaag gccgagggtc aagcacagtt cgtcattcgt
     5161 gtgctggtaa accgagggtt gagcatttcg gaagctcaac gtgaacgcat actcacctgt
     5221 cacgatactg agcagctcac tcgttggcta gacgcggcac tgtcctgcgg cagcgtggac
     5281 gagcttctgc actgagacct tgttggaaga aacgttcgct acctcatcgg gggatcgccg
     5341 ttggctctcc ccaccgtttt gcggtcatca ctcaagtgag cgaactcgat gagctcgcgg
     5401 ttgaggcgtt cggtgtgggt gaagagcagg ccgtgcggag cgttttggta gatggcgagg
     5461 cgcgcgtcgc ggatgccttc ggcggtgcgc tgcccgagcg ccagtggcgc tgagacatcg
     5521 cgatcgccgt ggatgacgag gcagggcacg tccacggcct gcagatccgg gagtagatcc
     5581 gagtgtgcgg cggttcggct ggtcgcgagc agcacgtcga ggtgagtttc gagtaaaaga
     5641 cccgcgaccc aatccataat gggtagcggg gtatctggca tgaagaacgg tcgcttgccc
     5701 gcctccaccc actcggcaaa gtcgtgccgc cagctcgccc acagttgctc gaaggcagcc
     5761 aatggtaaac cctcggtgtt atcttcattc tgcaacaaga tcggtgcgag cggcgcgatc
     5821 aaaacaacac tgcgcactcg agcggagcca tagcgcgaaa gatagcgaat gatgtcggcg
     5881 cagccctggg agtgcccgac cagtacggcg cgctggacac cccgttggtc gagcaccgcg
     5941 tcgaggtcat ccgcagcagt atcgtagtcg tagccggagg ctgccgggct cgagcggccg
     6001 tggccccgcc gatcgtagct gagggtgcgc agccccgcgt cctggagcgc gcccgtttga
     6061 tattgccaag cgcgatgcga caaaccccaa gcgtggacca agacgaccgg cggcccgtgg
     6121 ccgacgtcgc gacaatatag ggtttcgcca tcgcgggtca cgatggtacc cgggctcgga
     6181 ggagagatgg ggcctttggc agagacgttg gacataacgg caccatgcag cgcagctcgg
     6241 cgagccgcga ttacctcaca ggtaatcgcg agagggggag cacctgtcgc ttcggccata
     6301 gaacgcactc tctctgtgct tagcctcgct gaggagccgc gttgcacgct caatcgtgat
     6361 gattgtgcgc tagccattcg ctagtcagat ggagagtcaa gggcgccatg cggcgaaatt
     6421 ctaactctct agtttggtct gtgttcttgg gatctatccg ttggggaatc gtgagccaag
     6481 caagaggctc cagggaatgt cctcaagtcc gttttggttc ccgacttccg ggatatcccc
     6541 tgcgccgtag gggcgaaatc tgatctgttc gattgtattt ggttgctgcc aatcatcagg
     6601 tttacgtata gttctcgtca gtaggtcttc atgttgcgag aagcggtctt cgtcgcaata
     6661 gagcggcgct gtgtctgagt tcagaatagg atttagacag agaggggaac ttcatgagag
     6721 caaagcttgc gtcggatgcg gatgccgatg agcctcaaat cgaggtgagt gaatgttatg
     6781 acgtgcgtgt ggtggagctc gcatggctga gcagtcgcat gctctggatc tggggtgggg
     6841 agaaactcgc acccaatccg agacggcggg aaggcctcgt ggcggcctcc tatcgttgtg
     6901 aacgccacta cttgatggcg atgcgcttgc ttggcttacg gagcatgcga ggagctgccg
     6961 cggagctcga gcgggcctac gagatcgaaa atcaattcct gacctttctc ggagccgctt
     7021 gattgttcaa cgagagtcaa agggctatga actactgcac ttctcgacgc gagagtcgcg
     7081 tcactgcgta tgtcgttctc ctggtagctg ccggcgcggc atgcagtcgc ggttcgagtg
     7141 tccggggcga cggtcttggc ggaaccggcg gcgcgagctg cagtacaccg gatgcggggc
     7201 agggcagccg gcacagcggt ggtcgagaga cggagttttc cgcatccggc tcgagtggcg
     7261 aactggccgc agacggcgga gccaaagctc tcatcagtga ttccagcgcc ggtgaggcca
     7321 atcccgagac caactcagct ggcgaagaca acggggctgg gaatggcggg gctcgcggcg
     7381 atgtcgataa cgaggtgggt ggttccgccg gagccagtgg cggaggtgcg ggtgcggccg
     7441 gcacacccac cgagcccgaa tctcgaccgt ggaagctggt cttcatcacg ccggatgcgt
     7501 tcccttcgga tttcgggcga gacgggagtg agcgaggcgt cggggctcac gtgcgcgccg
     7561 acgcactctg ccagcaagcg gcaatcgacg caggcctacg gggtcagttt cgggcttggc
     7621 tttccacgag cacctcggcc gcaatcaact actttgccat cctggatatg aatgggccgt
     7681 ggtaccgagc cgatgacgaa ctcgtcgcag aggacctttc agcactcgtg tccaaaaacg
     7741 gcttgcgcgc acccatcctc tacacgccga acaaagtgcc tgggggaatg ttctggaccg
     7801 gaactggtcc actcggcacc ctcgccagaa actgcgacga ctttacgaat gccaatttga
     7861 acgacggcct tgccggtcat tgcgcgctcg cgaccggaca agagtacgga tggccgaaca
     7921 ccgccttcat cgcatgcggc tccacgaacc gcgcgctcgc atgcttcgaa cggtgagaat
     7981 agtctcgctc tgggctcatt cagcgggccc ctccgcaggc gcgttgggct ccgcgactgc
     8041 ctcggataca gcaagatcct ccggcagtgc agcggatacc gtgatacgac gcggcgctga
     8101 gtcgcctcgt ggtggggcgc tacgagaggg ttcgcggtct gcgggagtgg aggttacatg
     8161 ctggtcccca gagctcgcgg aagggggcgg cgtcgcgcct gcacgagggt ccgcgtcgga
     8221 ttcacctgaa gatcgtgatt cgagagctgc gaggtgcagg agctccggtc gcccgaagcg
     8281 gcgaaacagc gtgctcagtg gcacctcgtg cacattcagg tcctcgggta cgagcacttc
     8341 gattgaacgg tgcgcaacct gatcggcgcc aacatgaacc gtcaccgtgt atgagccgtt
     8401 gcgcggccga gtgagactga gttccaacgc gagtaatccc ttggcgcgta gaaagcgatg
     8461 aacgggctct ctgaacccaa gcacgccccc gctctcgaga ttcgccacgt cgagctcgag
     8521 ttgctttacg attagcggtt cgccttctgg cgtcggaacg cgcggaggct cactgattgc
     8581 ggcttctgcc acctccagcg cccgctgcca aggttcgcga actttgattg acgaaccccc
     8641 cgtcaggctg caactgggcc gcaaccgcac tcggtcagtc gcacggaata ggatcacact
     8701 cgggtccggt ggagcttcat aggttacccg cgttacctcg ggtgtacgga gactctggta
     8761 ctcacacgtg agcttgaagg gctcgtttcg agcgcattcc gttgcccgac acaagtcgtc
     8821 agggttcgaa gtcttgcgat atcgagtgcg cccgcagacg aggtcatcgt ccgatccaac
     8881 gtctgtggcg acgtagaccg gcggggagag ctctccgcct cggatgttcc gcccgtgcac
     8941 cgacgcgtcc acgacatcga gatcccaagc gcgcggagag tcgagtagca agagcgtgtc
     9001 attgaggtcg ctgatcggca attgtttggt tgcggtctgg aactcgcaat ggaaaccgac
     9061 ggccgggcca tcccccacat tggtgatcga aggggctggc aacattaccg agaacggctc
     9121 atcagcgagc tcgaacttcc cgcgtgtcca ggagacttgg agttgtggct cggtgggcag
     9181 ctccttccaa ggaaacggtg cgctgtggaa acggacgtct acgctgaccg ccgagagcat
     9241 gagtgagtct gcatgggagc gattggacaa cacctgcgat agaacgtacc ggagtggcat
     9301 tctcttctga gctcgcaagt ctcccgagaa gtaggcccga gagaaagtct tgaacgcggc
     9361 tcgccgcacc tctggagcca acacattggg cgcctcggcc gctggaaagc cggaccccac
     9421 gagtgtcaac gcctgctccc gttcagacaa ccccgtgggc ccaactctca gagacatccc
     9481 gatcagataa accccgattg caggaagtgc gcacaacgga gccagccaca acttgagtcg
     9541 cgaaggcgca tggttcagcg tcaactccag gcgggcaagc tgtgcactgg atcgcgaaag
     9601 cccgactaac acgtttgtca gcgttcgctc tgctaatggc ttccccagct cggcttccag
     9661 cgcatccaat tggtgctgga tatcagtggc caccgctgtg agctgccccg cgaccgcgga
     9721 gctccggtgc ccgttcagag ctcccaggcc gaccacgtcc acgctagcca gcgcgcaccg
     9781 taatgcgagc tccgtttcgc gatggcgcgc acgatcatgg gccgaaaccg cttcgcagag
     9841 ccgctcgatg cgctgccgga agtactcgac cagcgcccgc tccgcgctga gcttcgagaa
     9901 cagggcttcc accaacgatt cgactgccgg caagttcatc gcgccagtat gagggccctg
     9961 gatgaattcc ctatagtcat ctgatcacca ttccgcgaat ccctcgctca acatgggctc
    10021 agaaatttct gcccgtcggc gcgctcgctc ttgcgtcggc aatatccggg gtggcgcagg
    10081 ccgacacggc caaatgcgac ctccgcgagt tccaagcaag ccaggtcgct cccttgatcg
    10141 agcgacaatt caacggtcgc ggcacgactg aacgcatgct tggcgctcaa gtattcgttc
    10201 ccgcgcagcc cgggctcacc gcagagtggc tgcgcagccg ggtggagcaa cacgtttcgg
    10261 gcatgcgcca cacgggcatg gctggctgcc cactgggcgt ctcgaacctg agagtggcgt
    10321 tggtatccgg gggaacgggc tgtgcaaaaa agaactccgg tgaaggcgcg cgaggtggtg
    10381 gcctttaccg gatatggatc ttgaatcggc gatgttgaag gagctgtttg ggcggcggca
    10441 aagcggacgt gcgcgacgcg cgtcaccgtc cgtcgtgagc gcagacagat cttcaccaag
    10501 ctacccgcat ccaccgcgct caccgcacgc aataacgtca tactttgcta cgcctccgtc
    10561 gtttggcgtc agcgctttcc cccggtcagc acgcaccgcg acaacggtga gattccgcgg
    10621 cgcagccgcg cagcctgggc cgaggctttg gcgagcatcg gagggaggtg tacgaggcat
    10681 acgagagcat cctagatgcg tagcgacccg tgaagcagcg gggccaatca gaccaggacc
    10741 agatctcgga cgcctaaggg ttatctgcgc gagattgctg agcgttcaac cagctgttcg
    10801 agagcgatcg cgctcgcctc gttcaccggt gaccacacga acatggagag gccgtcgctg
    10861 ccttccacgt agaagacgga ggcttcgagt tcgatctctc cgacgtcggg acggatgagg
    10921 cgcttccgga actcgctgtg cgggcgcatt tcgccttcac gccacaggcg acgaaactca
    10981 gggctcgtct ccgagagttc gtcaatgagt gcggcggcct cttcggacgt gccggcccgt
    11041 gcgatgtcga cgcgaaacga tgccacacaa gcgcggcgca cgacttcgac gtgctgcacc
    11101 ttgttttctg aaccgggttg aaagactcgg cgcagcaggt tgcggtcgcg ctccggaact
    11161 tgcgagtagt caccgagcac cgcgacggcc gctcggttcc acgccacgac ctgaaacgtc
    11221 ggggtcttga tgaaggctgg aacagagagg tgatcgagga cgcgctgaag ggatggcgtg
    11281 actgcggccg gcttagtagg tttgagcggt ggcgggcgcc cgtgcgcgag cagaaacaga
    11341 atctcgcgat tcgtggcgtc gagttcgaga gcatcggaga gccgctcgag cacgtcatcc
    11401 gacggcatgc caccgcgacc ctgctcgagc caggtgtacc aggtgacact cacaccggct
    11461 cgggccgcga cctcttcacg gcgcagcccg ggggtgcgcc ggcggacggt ggtcgtgggc
    11521 tgcaggcgtt ctcgacggtc gcgaaggaac gccggtaggg tggtagtcgc cataccggta
    11581 ttcgggctcg tcttttctgg catctccttc gagcttacgg ttcgcccatg acgaagacaa
    11641 tgcgagtgtt tgtcacgggg gcgacaggtt ggattggcac ggccgtgctc aaggagctgc
    11701 gtgcggccgg ccaccgcgtc ctcggtatgg cgcataccga cgcgggcgca gagaagttgt
    11761 ctgcgcaagg agtggagatc tatcgcgccg atctcaggga gcctacgagc ctcgtcgaag
    11821 gcgtccgcaa aacggacgcc actatccatc tgggcttcca catggatttc tcggatctct
    11881 cgaaaatgta cgaattggac cgtcaggcga tccggaccat gttggacgca atggcgggct
    11941 ccaacaagac gttcctcggc accaatggca cgctcgtcgt cgcccacccc ggaaagctcg
    12001 tttcagaatc agacactgaa cctaccaatt cagtgctctc cgttcggctc tcggccgagc
    12061 gtctcgtgct cgacgccgtc aagagcggag tgcggggcag tgtcattcgc cttgcgccaa
    12121 ccgtgcacgg ctcgggcgac aagggattca tacctatgct catccaactc gcacgtgaga
    12181 aacgggccgc cgcctacgtc gacgagggcg gcaatcactg gcccgcagtg catcgagacg
    12241 acgcggcgtg tttgtatcgc ctggctctag agaacgcacc accaggaacc gtgttgcatg
    12301 ccaccgcgga agagggcatt cgaatgcggg agattgccga aacaattagc caaggcctcg
    12361 gcgtcccgac caagagcgta cccgccgcga ccgccgccga ccatttcgga tggctgtcta
    12421 tcgcggtggg tctcgacaat cgctcatcga gcgcggcaac acgccagctc ctccgatggg
    12481 ctcctcaggg accgggcttg ctgcaagaca tgcgcgctca ctacttttga ggggcgtcgc
    12541 ggcggtgcac cgcgtccatg gtcagggcta cgctgtagga cggcgatgag gaactctcac
    12601 gcgatactct gccacgacga gattcagagc ccagcccgcg cccatcagga gcgcgtaagc
    12661 aatgccggcg ggtttgccga acagcaacca ccacggaacc atgatgagcg cttgcgttcc
    12721 ggcgccgatc ccaatggcgt aactccgcac catccaggct cggtgcgccc tgatgtcacg
    12781 acgcaagatc gctcgccagc cgagaacgag cgtcagcacc attcctaaac ccagtacgac
    12841 ccgaaaccct ttcagcagag gatgggcgtc cgccaaggga agcggataaa ctaagcccat
    12901 ccacagaccg gagagcgcgg ccaccacccc cgccggaaaa accacacgtc ccacgactcg
    12961 atgccagcga gaacctcggg tgaacggcgc gaactgcagc gcacccagga ggcaaaacag
    13021 cgtcgcgccg agcacatgga gcacgacggg cagcggactt gcaatgaagc gtgcgttttc
    13081 ggcgttcgcg gcgacgcctc ttgcaagttg cgacatcctc gctgccccgg cagccagcgg
    13141 caccaggctt agaccgatca acgcggtcgg gacgagccat ggacgaggag gagcttcagt
    13201 cacatccatg gtctgagcat ggcgtgcaaa aactcgcggt cgatgcgcga ttccgtgacg
    13261 catccgtgca attatgtgca gcgtggattg ggggcttggg gtgcggctct tccatcgaag
    13321 ggacgatggc ttcatggcta cggctgctgg cgacgaactc gcgcagtctg ccgtgcgcat
    13381 cgaagaagag atcctcgtca gtgaaggacg catgctgggg cgtgatgccg agctccgcgg
    13441 gcggcttcgc atctcgacgg tcgacttcat cttcgcgggc tttccggacg tattcgaaag
    13501 cttcatccgg cgctatccag gcgtggacct caccgttggc gtcagcaacg agcgcgtctc
    13561 gctgattcgt cgcgaggccg acgtggcctt gcgcttggtc aacagccctg gagagcgact
    13621 cgtagggcgg cgcctcgggc gattgcagtt cgaggcgtac gcggcccgcg ccctcgtcga
    13681 gcgactggga agacacgcac ccttatcaga cttcccctgg attcactggg acgagcgctc
    13741 cgacggcact tggctcgatg cttggctcgg aaagcacgcc ccgggcgcca gaatctcact
    13801 gcgttccgac gactacgccg ttcgccgccg cgccatctcg agcggcattg gcgtgcactt
    13861 cctcgcgtgc ttcgacggcg acgcggatcc agcgctagtt cgtttgggag cgcgcctgag
    13921 cgacgaggcg cgtgacctct gggtcctaac gctacccgaa ctccgcgcca acagccgcat
    13981 ccgcgccttc atggaacacg ctcacagctg cttcaagcgc cacagacgag cgctcgaggg
    14041 ggctgcgacg cgcggaacat gacagccacg aaaaagaagg ttgggctaga gctcggagct
    14101 accgatagat gagaacgtcg acaggtccaa tactgagttg tctagtcttc gtacttggct
    14161 gctctcgaac gcccaattcg tcaggccagg agattggtcg atcctgttcg cggcggtgcc
    14221 cttgggcgac ggcgtgccct gtttccagag cgccagatga gtcatcgtag atttgcgata
    14281 ggtcatgtct tgttgcggtg cggcacccga gcgtcggctc ctctttgcaa tcagcgcgtg
    14341 gccagaaatg acgctgattt acagagtttt tggggtgggc gaggggtggc gccggtctcg
    14401 aagatccacg tcgtgaacca caacgcgatc ttgctgcgga gagagggacg ccaaacggcg
    14461 ttttgcgcca atggctatct tttcgaactt gttcggagaa ttggtctgag tcagacaatc
    14521 gataagaaac gatgacccgt tggagcttcg cggagggccg ccgccggctc gaagtgcgct
    14581 cgcagctcgc cgtgctcgag gaatgcgagc tgttggtttg ttcatcgttt ccgcgcaagc
    14641 gtagctgccc gtcagggaaa ctcgtcgtcg ccatcctgga ggtcgtttgc gctttttgag
    14701 catgtggagg cgcagcgcgc tgggcgcgag aaattctatg cgtcctatga cgtgcgggtt
    14761 tgcgggcgtc gagcgcgcct atgaattctc gatggcgcac gtgccacaac gtgtgaccag
    14821 agtgggagcg tgacgcgatg accgtttagc tttgcggctt cgggttatcc tagcgccagc
    14881 agaggcgccc aaacgcggct cagtgcggta cgacgttcga gcgcgcgtga attccgaggc
    14941 gcgtccccga ggtcgggtct ctctgaagct cctctcgaac gccggcgagc gcggcttcgc
    15001 aagaacgtgg gctcttgaag tcgaatgctt cgctggtgag ccaattcgac acgtcgccaa
    15061 gacgaacgaa gggacgcttg cggaccacgc ggcggagcac gctcacagct gcttcaagcg
    15121 ccgcaaacga gccctcgagg gggctgttgt gcgcgtacta tagctcaacc ctctttttcg
    15181 tggctaagct gcccgtgtat accgcgagat cgttggcctt tgaacgaagc ccggtagcct
    15241 caccggccct ccgacacgta gcggattcaa cgggccgtct aggctgctag agtagtcggg
    15301 ccatggccga tcccgcgcga aagcgagcct cgtatagcga tcttcacagc gttcccgacc
    15361 atctagtcgc cgagatcgtc gacggtgagt tggttaccag ccctcgtccc gcaccgcggc
    15421 acgcgcgagc ggcgtcgagc ttgggagggg agctctacgg gccgttcgac aggggccggg
    15481 gaggacccgg gggttggagc atcctcgatg agcctgagct gcacctgtcg ggcgacgctc
    15541 tagtctctga tctaggcggc tggcgtcgtg aacgtatgcc cttacttccc gaggtatccg
    15601 ctttcgagct tgcgccggat tgggtttgcg aagtcctctc tccctccacg gcgccgctcg
    15661 atcgcacccg aaaactcccc atctacgctc gcgagcgtgt ccagcatgtg tggctcgtgg
    15721 acccgtcgtt gcggactttg gaagtgctgc ggctagacgg cggaggctac cggatcgtag
    15781 gcgcgtggca cggcgatgcc attgtgtgtg ccgagccgtt cgaggcgtac gcgctgcatc
    15841 tcgccgactt gtggtcagcc taactaggaa caggaccatc gctccggtgc catgaccagg
    15901 agaggctgtg aacatccgct tgcaagtaag aattattagc ctccgtggca ccgtgctgga
    15961 tgcagggatc cagttcaagg atgagataac cctattccac cggttctacc gctaccgcca
    16021 ccttcccacc agtgtagtag accgcgtaat tcgcgcccac aatcacgagg gtcgtaactc
    16081 tctcattgat gctccattcg agctgaaaga gcttttggat accatgacct gaaaaacaca
    16141 gatgtgaccg ctcctcggga ccgatgtcct tcgacaacag ttgacctcga aacgcaagca
    16201 cccgatgccg ccgactcgag atcaaactgc gatatccgat gatcgccgcc caaaggccaa
    16261 agattaaggt ggcacttggg tggccactaa agaaccaagc ggctcccgct aaagaggcta
    16321 gcagcagcgc aaagagcact gtcacccaaa cgtctgttcg ccacgagtca gcctgtcgtc
    16381 tcgtcagttg cccacgccga ttcgcctcta gatcctcgtg agtgagctgc agcgcgccga
    16441 ggagttcgtc ccggtggcgc tgaagcatta cgtccttggg ttctagctct tcagcggaaa
    16501 gcacgagctt tgaacgtggt aagtagtaga agttgtagcg cccggggtac agaaccaacc
    16561 cgtggcatgc gcgcaggccc ttgattgcct ctgcgacggg ctcgccatac cagcctttgc
    16621 cactcgccac tgccaccgca ccaaaagaca agtcactttc caacttgctc gcgaaggtgg
    16681 cctcggtaca cctaagtaaa aacgttcgca ggttctcgcg ctgttgcggg ctgagccgac
    16741 ctgcccggtt ccaggatagg tcttccggtg tgctacttgc ctttgcgtct gctggcccca
    16801 ttcggtgcaa gcacatgcat atcacagcca actggctggg tcgcgctcca cgaacttgcc
    16861 gggctcacta gatgatttga cggctgcgtt cagaccaata tcagagtcac gtagacggcg
    16921 gctgtgcagg tttacggagc agtgttgaag cgtggggcga ctgaaaccgt gtccgaacga
    16981 accgcgcgaa ggtcatgact ctgtccgcag ccacgcttgt acgtgcggga cgcacggaat
    17041 gtccgctgct gcccagtcca ggtgcgcgag gtcttcgacg gttgcccaga tgacctgcga
    17101 gtgctcacgg agcacgaggg ttccggagat gatcgtggcg gcgaacactt cgagcgagat
    17161 gaggcgtgag ccgaccggag ctgtgccggt ggccagccgg cgtttgacgg cgatttccaa
    17221 gccgagctcc tcggcgactt ctcgagcgag ggcggtggct gcggattcgc caggctcgac
    17281 cttgccgccg gggaactccc atttatcggg aagtgtcata gacggccccc gccgggcaac
    17341 gagacacctc ccggactgga agattgcggc gccgacaacg tggatggtga ccatcggagg
    17401 aaccttaacc cagctgtgaa taagcgcgat tcaatctaga ggccctaacg ctcgctgtcc
    17461 acgctcggta cacatgcatt gaagtccgcc gctttcggcg tgggggacga agccaaagtc
    17521 acgcggaggg ctggtgccgc tccaaggaac gggcgcgaag cgcggccggt acgtcgtcag
    17581 tgccccgcct cgcggaaggg cctcgccgct ctaccgcgca agcggcgcac cctaacgacc
    17641 gctaaactca cccggaataa caaggttacg gggcccgccc gccgtgccta cttggccccc
    17701 tcagcgtcgt tcgagcagct tctcgagctc agcaacgcgg gccaacgcgg cttcggtgcg
    17761 ttgccgttcg gcctcttctt gagtcggaaa aagcacttca ccatcggggt cgagcccaag
    17821 tcgcacgtgc tgccgcggct cctgctcggt gcccaccagg cggatgaagc agccgagtac
    17881 ccgtgaacgc acgcggtccg cctgagtacg ttcgcggagc tcgaggccct gcgatgttcg
    17941 ctcgaagcgc tgaaaacgat ggccctcgcg ccgtttcgaa tggtccgggt cgacaacgat
    18001 gagctcctct gttccgactt cgttgtggag ctgcggcgcc gtgacgtagt ccttctgcca
    18061 atcctgagag cagatctcca aggcgaagct cggacgaatg ccgctctccc atactttcca
    18121 ggacgggatt cgagtgttcg gagggactcc gggcaagaca tagatgtccg gagcgatgcg
    18181 cttcgagacc tgacccttct cgaagtagat gaactgatca gagccgacta gccacggagt
    18241 gccgcgctcg gcgagccagc gctcgatgag cgggcgcagc tcttcgagca tccaacattg
    18301 caggatgtct tcccccacgg gctcctccac gggatacacc gtcgagtctt cgagagaggg
    18361 ggccggagtg cgcatgagac cgaacgatac cacgcgcgtg gcgccccgcc gaccggcagt
    18421 gtgcatatat cctcatctag cacgagacta taagaagacc tgaacaaaag aacacaaaaa
    18481 cgccgaggaa ttcgggtcta tcccgcaggc cgaagggagg cgctcgggat atcccgagcg
    18541 ccgtcaattc gagatcgctt tcagctcgcg atctggagag cgaacacgct gacgcgctcg
    18601 cgatctgcac cacaccaatt cggaacaagg ggccaggcag ttattaggcc gagccgcgag
    18661 gattccggat tcgggctagt gcggcgccca gctcggctca gtgcggcacg atgttcgagc
    18721 gagcgtgaat cccgaggcgc gtccccgatg tcgcgtttcg gtcttcccac aacacttcga
    18781 accatcgacg ctgcttgata cagaatcctt cgagtaggta agcccgccca ggttccaatt
    18841 ggcctatccc gcgcgctgaa ctaccggagc caaggtagct tgctcagctt cgagtcgcgc
    18901 atagcgttga cgacgcggaa gaaacgtgca actcatccca gtatgtgggt gtttacggag
    18961 ctggcggatg cgtgggatcg gctgcgggaa gaagacgagc agttacttga cgcccaatgg
    19021 tcaagttgga cgagatatgc tctccagcat tcggatgacc cgcactggta ctcttcaatt
    19081 cgcaacctcg ccgtctattc tggggtgggg ttgtcctacg ctctgtggaa agccagcaca
    19141 agcagcgcga cgggattcat tgatgttttg cgcctcggcg atgggatccg gcagggcggt
    19201 tggggctacg gccgcgacgc actcagactg ctcgttctca ccggccctgc gctccgctta
    19261 gcgcgattgg gtctcaccaa actcgccagt ctcgacacct tcgaggccaa cgccaactgt
    19321 gcatgggttg ccgcggcagc cgcgttgcgc atgacgggca taaagcactt tgcgacgctt
    19381 ggcgacttgg cacgggcggc agagatgccc gtcgcgggct taggtgaggt caaagatcta
    19441 tccgtggtcg ctcccttcat tcagcgtcta ggtggagtca ctcgctttat cggaaccccg
    19501 tcagcggaag cggagcaaac gatcgcacgt acagttgccg ccaatccgaa cagcgcagtg
    19561 ctcttcggcc tgagatggga cgttggcggg caacaggtag ggcacgccat ggcggcgttt
    19621 cgtgtccttg gcagaacgat gatgctcgat cgtgacggct ctatttatca gaatctcgct
    19681 cagatagaag cgcttggcgg acaggccacg ggcatatcaa aggcgtttgt gtcagagcta
    19741 ctcatcgtcg agaacgcgac aatcgcgacc gccgtaagtc ggatctcaca gggcgccgca
    19801 atcggcaacg cactcgcgag cattcggaaa gtgacaccga gtgcgtctga gcatcgcgta
    19861 tcggcatcaa attcctatct cgcaaagtcc ggcgatttgc ttcctctcat cgagagtatc
    19921 gggcttgaag tccggtccgt tccaatgaga tccgaaggca gcctaagctc gaactcacgc
    19981 aagccgcgcg gagcgagctt gtccagcatg aaacacaaaa taaccacatc ttgcattgat
    20041 cagtcgaact ccattttcct gaaggagggc ggccgcgcgg caatagcaag gcgttggtgc
    20101 ggcaacttga ccgtagacta caaagtctat gtggtgcgac cgggggactt tctcgccagg
    20161 atcgcaaaga ttgcttacgg cgatgagaca aagtggccag tgttataccg agccaatcgc
    20221 acgaaactgg gcccaaaccc gcacaatccg acggcactcg ttccagggat ggagctcgag
    20281 gttcccgtct ctgcgagtca acgcatctgg gttgaatgac gcgatagcgg gttcaaaaat
    20341 ggttcaccct tggggttaca tcgattatgc aatcaggctc gaccgtgatc cgggcggaca
    20401 ttggattgga ataggcgtga gtcgccaact cgagcagttc ggatacccgg gactaatggg
    20461 attctcgccc cggatcgagg tttccgctga ccgaatccaa gtgtccgcaa actcgaagga
    20521 gcgccgaccc gaccaatgga tcgatcttgt gttcctccct cttccctttg cgaatcggcg
    20581 ctggcaaaaa tacaagagga gtcgcgcttc gtgtcgaatg atcgaggtca atgcgccggg
    20641 gctacaggaa cacttgcgca ttgggcgcac gctcctgctc gtgcgtggga gagagctgtg
    20701 ccacagcccg aatgcaactg gactgattga tgtaagcgtc cttgatggcg agcgcaccgt
    20761 attcgaactg cgcagcaggt actgcgcaag tacgtggtag aatctgaatt gcgtcgtggg
    20821 gcccgcagcg ggtgtcgacg agcacagcgg tgcgagatcg agacggacgg agatagtctc
    20881 ttcggggctc ttgatagaaa gcggccagcg gatgcatccg ctgaccgctt ctcgtcacct
    20941 cgcgtgcgtg cgaaggccgg ttgccgtcat tcgttccagt tcttccagcc ttcccaattc
    21001 ttctgcttgg cctgccagac gccggtgttg ttgtagtccc caccgctgct gaggaactcc
    21061 tccttgcggg agttctctcc cccaagatgc cagcgcatcc aagcaacatt ggcactggcg
    21121 ccgtcccaag gacccgagcc atgaccaccg cccttcatca tgccgagcca cactgggccc
    21181 ttgttgccaa cattgttgaa gtcaccctgg gcgttcggcg cctcttgacc cgtttcaccg
    21241 tagatgatgc cgaccggaat cttcaagtta acgagggcgt tgtgctcgaa ggagccgctg
    21301 ttatcgagca atgcggtgga cacgcgcttg tcggtttgag caactgcttc cgacgagagc
    21361 ccgcccatcg agtgcccgaa tacgccaacg cggttgagaa tcagacggtc atgcatgggg
    21421 ctgctcggat tggcgtcttc gtcttctagc caatcaatcg ccgccgacat gagcacggcg
    21481 tcgcccgtcg attctcgaat cccgacgatg acgaaccctt gcgaggccat acgcgtcagc
    21541 agctgtaggt aatcgcgagg aggggtgcct ccgccggggc cccaaatggc cactggatgc
    21601 ttggtattgg gctctgaggt gagtgtcgtc ggcctcacca aaatgtggcc gcccccactg
    21661 aaggagaggt cctgaacggt ggtctgaagc ggcccgttgg cgttcacatt gtcacccgcc
    21721 agaaagacct tagtcgtatt gacttggccg ccgctcccgc ccgagccagc ggaggttccg
    21781 ccagaattcg cggagctccc gcccgagccg gcggaggtgc cgccagaatt cgcggagctc
    21841 cccccaaggc cggcggagct gcctccggaa ttcgtggagc tgccaccgga gccggcggag
    21901 gtgccgccag aattggcgga actcccgcca aagccggcag agctcccgcc aaagccggcg
    21961 gagctgccac cggaattcgt ggagccgccg ctcgcgctcg tgcttccggc ggtggtagtg
    22021 ctgcctccgg agcccgtagc agtaccgccc gagctagagg cagcacccgc gcttgtgctc
    22081 ccggcggtgg tagcgctgcc tccgaagctc gcggcaccac cggagctcga actgctgccg
    22141 ccctttccgg gcgcttctcc gggagctggc acgctcgagc atgcgaggag cagaaccatg
    22201 aggccggaag gagctaattg cctttgtctc atgtgcattt acccccctcc agatccctgg
    22261 tgcgctttct cgaggacgcc cccgggactg tcggctcatg gggctcggga acatcaaatg
    22321 accccagggg ggcaaaaaac tgcgatctga gtgcaaacat agctggtacc cccagcagtg
    22381 ttgccgaaca ccagtttccg tcgctctgcc tgggattgat gtaggtcagg tcgcagggcc
    22441 tgactcgcgc cgtgcctttc ggcatgtgcg tcggcttctc tcccagcctc gctgcgatgc
    22501 tgcactgcga gttttgcgtt gctgctctta acctctgccg cgacgatcca atagagttca
    22561 cctcgatcga gacggacgga gatatctctt cggggcgtgc aaaggcgtgc tgtgatctat
    22621 gcggctagac cgcagtggga ttcgagtccc gtattcgcgc gcgtcggacg gactccccgg
    22681 ctggagactg gtcgctgcag tgcgatgcgg tgctcttgag cgacggagtg gcctgtcccc
    22741 ggagtaccag atgagtcatc gtcaatttac gataggttat gtcttgttgc ggtgcggcat
    22801 tctgagcgct ggctcctctt tgcaatcagc gcgtggccgc aattaaccga taatttggag
    22861 ggtttttgcg gtggcgagag ctggcgccgg tcccggagat ccacgttgcg aatcacgacg
    22921 cgatcttgct gcggagaggg gcgccaaacc gcgtctggcg ccaatggcta cattcccgaa
    22981 tttgttgaca gaattagcct gagccagaca atcgataaaa aacgatgact cgttggaggt
    23041 tcgcggagcg ccgccttcca actcgaagtg cgcgcgcagc tcgccgtgct cgacgaatgc
    23101 gagctgttgg tctgttggac ggcatggttc gcgctgcgct gggtcgccat gagaagggtg
    23161 agctcctggc ttgcgcggtc gaggtgacga acgcattggg gctgagtgct tcggacgaac
    23221 cggtcgcggg caattacaca gatgtacgga gccgctgtca cggccggcac ggctgaagct
    23281 ctctacacag tggccggcac gctcggtcaa ggtctagtcg acttattgag agtcggggaa
    23341 ggcgtcaagc gcggtacggc tctcggcgtg gcccaggatg cgttgcgcgt gataagcgta
    23401 gtcgcggggg gggctacgtg ttgcccgcct ggctggctca gtggtggggc ttgggggtcc
    23461 gatgacgtgt acgctctcct cgtcggccaa agccgccttc ttgagtggca atacgttccg
    23521 aatggcagcc ttgttcgatc gctttgcgct tgggacactg cccaaccgcg aacgaactcg
    23581 atgagctcgc gattgagccg ctcggagtga gtgaagagca gtccgtgcgg tgcgtcttgg
    23641 tagatcgaga gtcgcgcggc gcggatgccg acggcggtgc gctggccgag cgccaagggc
    23701 gctgagacat cgcggtcgcc gtggacgacc agacagggaa catctaccgc ctccaggtcc
    23761 gggcgtagat cggagtgcgc ggcggttcgc ttgcttatgg gtggccccgg cgcaacttca
    23821 gggggttgcc caacttctgc gttaggagag cttcgcgcac cggcgcctgg agctgaccgc
    23881 aagcgactgg ctcggcggca atggaacatc gccaaaccga aggtaaagcc gagggcaaag
    23941 ccgagttcgt cattcgcgtg ctgacgaacc gagggttgag cctttcggat gcttaacgtg
    24001 aacgcattct cacctgtcga gatggtgagc agctgatccg ctggctggac tcggcgatgt
    24061 cctgccgcag tgtggacgat ctcctgggtt gagcacgcac cgaaaactgg gctaccgtca
    24121 cgagccagac tgatgatagt ggggcgggtg agagcgaagt tgccgccgga tgccaggagt
    24181 tcgcgctgtt cgctgatgat cctgacaggt agcgtcgccg cctttatccc atctttctcg
    24241 ttctgggggg acgccaaaat gtgaacgagc gcgactgcag aaggtcacgt ccacgctcgg
    24301 gcgacgagcc cgcgatcgtc cacctcgatc gctagacctc gctgattcag gcggcgatcg
    24361 cactcggcga tctcagggtc ccacccgctt acaacggggc cgagccattc tgcgaacgtg
    24421 tcatcgaact ccggcaggta gtcgcccggc gttgcctgct cggcaaaggc gacgatgctg
    24481 ttgtagctgg ccatggacgt gacctacctc gccggctgct cgagcgtcca gtcgagctgt
    24541 gacaaacagt gtcagagctg gtagagcgtt ttaggtcggc tgagcttggg cggttcgttc
    24601 gaggtgacga aggttgattg cttttgtggc ggccgaaggt caactgggct cttgcgtgcc
    24661 cgaacagttg atgtggagct tccgggttgc gcgtgagtgc cgctctcttc gtcctcccgg
    24721 tgccagcccg cgacgccttc gtgaatccag ctcccgtcaa acataagata caaatccccg
    24781 atgtccccga ctgcggactc ggacggatca atggctgcgc gggagttgcg gagtgacgga
    24841 cttcgcgctg cgttatgccg gggcggaagt cctcgcgtgg atgggaaaga gttggaatgg
    24901 catgtctatt gggtgcgttg tgaggtggtg ttggtgggtg agtggtgttg gggtgcggat
    24961 ggaaggttga aatcgaccgg gggcacgccg attcgttcat ggctctgggt gcggtgtcgg
    25021 cactcactgt gaagccggat tggttcctag gtcctgacgc gccgttaaag gcgttacgtg
    25081 cgtcggctgc gtattcgagg ggtccatcat ggttgcgatt gcgcgtgagt atcgggtttc
    25141 taggctgatc cgctggcttt ggtgcggagc ctcgctggcg ctggcggggt gccgcgagca
    25201 ggacagtgcg ctgagtgggg acgagttggg gagctccgtt cttttgggga ctacggtggt
    25261 cgagtcggcg gcagggctgc ccgcgtgcaa cttggtgcgg cggggcggcg ttttctatgc
    25321 ccgcgctgag aacgcactct attactgtga tggtcgggct ttccatgctc tggatctgaa
    25381 tgggcgaccc ggtgaagatg ggcgcggttg ggcggtcgcg gtgagggacg cggcggcgga
    25441 gtgcgtctcc gggggggtgg tggtgagcgc agggcccgat gcgaatgggg acggggcgtt
    25501 ggatgaggtg gcgtcgacgt cggcggtttg cgacggcacg gcgggcgcga gcccgcctcc
    25561 cctgctgcga tctcgactgc ttgctccagg gccagagtgt ccgggaggtg ggcattgggt
    25621 tgagttcgga acggacgtga atgggaatct ttcgctggac gatgatgagg tgtcgagcgc
    25681 ggaagcgttc tgtgagcctc agaacctctg gcgcggggat gtggtggtag atggtgacgg
    25741 cgagctcgag gcgctgcgcg ggtttacgga gatgatcggg aacttgacgg tgaccgggga
    25801 tgtgacgaac ctagggccgc tcgagagcct cacgcgcgtg cacggcaggc tgcagatcaa
    25861 cgagagccgg gtgctcaact tgcgggggct cgcggcgctg gagcaggtgg acggagatct
    25921 ggtggtggac ggaaatctct cgctcctgaa tttgcaaggg ctcgcgagct tggcggtggt
    25981 gacggggcgt ttcagggtct ctcagaactg gagcctcgaa agtcttgcgg gcgtggagtc
    26041 gttgcagcaa gtggggaacc tctacgtgga gtcgaacaac ggtgtcgata cgacggcgct
    26101 tggaaacttg acccgagtcg acggggacat atcgctcgaa ggagccaaca tgcgcgggtt
    26161 gaggggcatg acgcgggtga acggctcgtt gagcgtgagt gttgcgggag tgagtgactt
    26221 ggaaggtctc gagtcgttgc tcgaggtcgg gggctctctg tatatttctg gatcttcgct
    26281 gctgaacctg aagggcctga gccgcttgaa gcgggtgggg gggacgctgt gggtcgcggc
    26341 caaacacatc cagagtttgg agggggcgga gtcgctgact gcgatcgggg gcaacctcga
    26401 gattccttcc cttagccctg aaatcatgac ggtccgcggg ctcgatggtt tgcagaccat
    26461 cggtgggagt ctgcagctgt actcggcgat tcgggacttg agcgggttcg ggagcttgga
    26521 gtcgatcggg gcggacgctg tgttggacgg gaacgcactc gtgaaccttc acgggctcga
    26581 gcgccttacg aacgtgggtg ggagtttcgt cattcagaac gcggggttgt tgcagtcgac
    26641 ggcgggggcc ggagcactcc gcgaaatagg tagagatcta cggatctaca acaatggctc
    26701 gattcgggat ttgcgcgggt tcttcgggct ggaacgcgtt gccggatcgc tgatcatcaa
    26761 cttcaatccg agcttacccg cgtgcgaggc ggagtggctg cgcgatctcg tgctcgggcg
    26821 acgcggagtc ggtgcggata tcgcaatctt caataacagc caagaaagct gtccttgagc
    26881 ctgagtcttc gaaccgagag gaggtatggc atgaactctt accccatgac agcatcgctc
    26941 gcgcgcgtgg ttctctcaac actcctgctg gttcaagcgg cctgcacgga gcgaccggaa
    27001 g
//
LOCUS       DASTKU010000778         4681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1046102, whole genome shotgun sequence.
ACCESSION   DASTKU010000778 DASTKU010000000
VERSION     DASTKU010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046102"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFQ61_27205"
     CDS             <1..527
                     /locus_tag="VFQ61_27205"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET9958224.1"
                     /translation="TDALCMEGMRRAARSLRRVVEDGGDLEAREDMAVVSLFGGLALA
                     NSKLGAVHGFAGPLGGAYDAPHGALCARLLPGVMLENVHAARALGLQEVVARYASVAE
                     LLTSRSGATPESGVQWITELVSELGVPRLSQYGVERAHFPELVRRARAASSMKGNPVE
                     LTESALTAILDGAL"
     gene            628..1590
                     /gene="argC"
                     /locus_tag="VFQ61_27210"
     CDS             628..1590
                     /gene="argC"
                     /locus_tag="VFQ61_27210"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET9958225.1"
                     /translation="MTARVFIDGQAGTTGLQIVERLTKRRDLVLLSIDEQRRKDPLAR
                     KELLESADVAVLCLPDEAAREAVELVASSAGSGSKVRLLDASSAHRVAEGWVYGLPEL
                     SRGQREAIRDAKRVSNPGCYPTGFVLLLAPLVEAGLVSSDAAISIHALSGYSGGGRKM
                     IEQWESPVQGLVGLPFEAPYALDKSHKHLPEMTVYSGLKNAPHFVPAVGPFHSGMRVE
                     VSLHRATLPSGTQAGRVIEAWTERYRNEPFVRVAPLDRKLDEFAFDPRRLNGSNHIEL
                     SVCGNPSGDLLLVAVLDNLGKGASGAAVQNLNLMLGLEEARGLE"
     gene            complement(1669..1917)
                     /locus_tag="VFQ61_27215"
     CDS             complement(1669..1917)
                     /locus_tag="VFQ61_27215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958226.1"
                     /translation="MDLAFLVLLAASFGAWVATHVALALWLFRQKPRFRAGLALILPP
                     LAPYWGLRGGLRGPALRLSLLWLISGFTYLISLIAGRS"
     gene            2121..4055
                     /locus_tag="VFQ61_27220"
     CDS             2121..4055
                     /locus_tag="VFQ61_27220"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HET9958227.1"
                     /translation="MRFTALLASAVVLGLLAPLPIQLRKAKAEDGTARADVLPIERIK
                     PGMKGYGLTVFQGTQPERFDVEVIDVLKNFRPKQDLILIKTKHPRLEVAKVVAGMSGS
                     PIYLENKMVGAYAYGWTFGAEPVAGVTPIRNMIEDLVRPLPDKIYGWPLRTLPVARNS
                     AREPGAVREPGSKLGALDAPSRFGMEPLSRYSGELAKYDVRQHASSLAARGSSSANTG
                     NMPALAPVATPLLVGGFTSGALAMSKELLAPLGLEPLAAGGSGVEDPNAPNRFVDGGA
                     IGVQLVRGDMSAMGLGTVTRVEGDRLVAFGHPMMEAGVTALPTAIGKVLWFLASDQRS
                     FKIGMPVRSMGAMVNDRQASIVVSQNTRAPLIPVSVKIHGMPGLPSANWNFEIAHERF
                     LSPSFVAVSLGSALQAVANEHQDVTWTARSKVQLRGRAAIEVEDYGLSMGGTPDPGEL
                     GRSNLVRAVGSILNNPWEPTFVDGIQVDLELSYARDVLRLRGAELLEPEVEAGQPARI
                     KLTFVPFSGPAQTRLVSVPLPAHLAGQTVNMELLPGYMADREQAAPDSLDELIHNLEH
                     PTYPPKSLVVEFSAGSGAVAFRGRVAKHLPPSALDALRPTSSTVAPDAFQTSNREVVL
                     LPKFVMGRDRISVSVRPVLR"
     gene            4103..>4681
                     /locus_tag="VFQ61_27225"
     CDS             4103..>4681
                     /locus_tag="VFQ61_27225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958228.1"
                     /translation="MRFLFGFRLQGLGAVAGGLSRVGSAQGRARVGACALVLATCAWH
                     APAHAVGTRHFVLDKGGDFKGGDLKGVAVDTAGGVRAGFNLGAAPMPDAATIWAALPQ
                     KDGSLLVATGNDGKLLRLAQGASSVVAETKALAATSLADAWVGTVIVGTLPEGKVMKL
                     DRGRLTELVRLKGAEHVWQVAFDPKAQVAYAAT"
BASE COUNT          767 a         1429 c         1634 g          851 t
ORIGIN      
        1 tcaccgacgc tctgtgcatg gaagggatgc ggcgcgcggc tcggtccttg cggcgcgtag
       61 tcgaggatgg cggggacctc gaagcccgtg aagacatggc agtggtgagc ttgttcgggg
      121 gcttggctct tgccaattcc aagctcggag ccgtgcacgg ctttgccggg ccgctcgggg
      181 gcgcttacga cgctccccac ggtgcgctgt gtgcgcgctt gctgccgggc gtgatgctgg
      241 agaacgtgca cgccgcgcga gcgctgggtc tgcaggaggt ggtggctcgc tacgccagcg
      301 tggccgagct gctcacctcg cgaagcggcg caaccccgga gtcgggcgtc caatggatca
      361 ccgagctagt gagcgaactg ggtgtgccgc gcctcagcca gtatggcgtc gagcgggccc
      421 actttccgga gctcgtccgc cgtgcgcggg cggcgagcag catgaagggc aatccagtcg
      481 agctgaccga gtctgcactg acggcgatcc tggacggcgc gctgtgagtg tcacgctctg
      541 agcgctacgc ccgtccccac gcccctcata cgccgccgca actttgcttg agtgttggtc
      601 gcgctatgtc atggttcgcg tacttcaatg actgcgcgtg tcttcatcga tggtcaggct
      661 ggcaccacgg gactccagat cgtggagcgt ctgacgaaac gccgtgacct ggtgttgctc
      721 agcatcgacg aacagcgccg caaggatccg ttagcgcgca aggagctctt ggagagcgcg
      781 gacgtcgcag tcttgtgcct gccggacgag gcggcgcgcg aagcggtcga actcgtcgcg
      841 agctcggccg gttccggctc gaaggtacgt ctgctggacg ccagcagtgc gcaccgtgtg
      901 gcggagggct gggtctatgg tttgcccgag ctttcgcgcg ggcagcgcga ggccattcgc
      961 gacgccaagc gcgtctcgaa tcctggctgt taccctaccg gattcgtgct cttgctcgcg
     1021 ccgctggtgg aagcgggcct ggtctcgtca gatgccgcga tctcgatcca cgcactgtcc
     1081 ggttactccg ggggcgggcg caagatgatc gagcagtggg agagccccgt gcaaggtttg
     1141 gtgggcttgc cgttcgaggc gccctacgcg ctcgacaagt cgcacaagca tttgccggag
     1201 atgaccgttt attccggttt gaagaacgcg ccgcatttcg tacccgcagt ggggccgttt
     1261 cattcgggca tgcgcgtcga ggtttctcta catcgcgcca cgctgcccag cggtactcag
     1321 gccggtcgcg tgattgaggc ctggacggag cgctatcgca acgagccttt cgtgcgagtt
     1381 gccccgttgg accgcaagct ggatgagttt gcgttcgatc cgcgccgctt gaatgggagc
     1441 aaccacatcg agctgagcgt gtgcggcaac ccgagcggag atctattgct cgtcgccgtg
     1501 ctggacaact tgggtaaggg cgcgtccggg gccgccgtgc aaaacctcaa tctgatgctc
     1561 ggcctcgaag aagccagggg gctggagtag cgagcggccg cggcgggcag ggcttcgagg
     1621 agggcctgga ctacgcagca aagagtaagg agccccagtg cgggcggctc aggaacggcc
     1681 ggcgatcaag ctgatgaggt aagtaaaccc tgagatgagc cagagcaggc tgaggcggag
     1741 ggcggggccg cgcaagccac ctcgaaggcc ccagtagggc gcgagcggag gaagaattag
     1801 ggcgagcccc gcgcgaaaac gtggtttttg gcggaatagc cagagcgcca acgccacgtg
     1861 cgtggccacc caggccccga aagaggccgc cagcagcacg agaaacgcca ggtccatccg
     1921 ttcgaggtct tcttagccat agaagcgcga ctgatccacg ggtgccttgg ctatccacgt
     1981 ttttcggacg agtaggcgcc gccgtgggtt cgaccggagc cgggcgttgc cggcaggcct
     2041 actccacaca tccttccagc ggaccaaaaa gtagcgggta ggtcgcgggc gcgggcgtgg
     2101 ttcgctagac tgagggcact ttgcgtttca cagccctgct cgccagtgcc gtcgttttgg
     2161 gcctgctcgc tccgcttccc atccagcttc gcaaggccaa ggcagaagat ggcaccgcgc
     2221 gggctgacgt tctacccatc gaacgcatca agccggggat gaagggttat ggcttgacgg
     2281 tgtttcaggg cactcagccc gagcgcttcg acgtcgaagt catcgacgtg ctcaagaact
     2341 tccgtccgaa gcaggacctg atcttgatca agacgaagca cccgcgcttg gaggtggcca
     2401 aggtcgtggc gggcatgagc ggaagcccga tttacctcga aaacaagatg gtcggcgctt
     2461 acgcctacgg ctggacgttc ggcgccgagc ccgttgcggg tgtcaccccg attcgcaaca
     2521 tgatcgagga tctggtgcga ccgctgccgg acaagatcta cgggtggccg ctccggacgc
     2581 tgccggtggc acgaaacagc gctcgcgaac cgggtgcggt tcgtgaaccc ggctcgaagc
     2641 tcggcgcgct ggatgcccct tcacgcttcg gcatggagcc ccttagccgc tactccggcg
     2701 aactcgccaa atacgatgtg cgtcagcacg cttcgtccct ggccgcgcga ggctcgagtt
     2761 cagcgaacac cggcaacatg cctgcgttgg cgccggtggc caccccactg ctcgtgggcg
     2821 ggttcacgtc cggtgcgttg gcgatgtcga aagagctgct cgccccgctc gggctcgagc
     2881 cattggccgc gggcggatcc ggcgtcgagg atccgaacgc gccgaatcgg ttcgtcgacg
     2941 gcggcgccat cggcgtgcag ttggtgcgcg gcgacatgag cgccatgggc ctcggtacgg
     3001 tgacgcgggt ggagggggat cgcttggtgg cgtttggcca tcccatgatg gaggcaggag
     3061 tcacggccct ccccaccgcg atcggcaagg tgttgtggtt cttggccagc gatcagcgaa
     3121 gcttcaagat cggcatgcca gtccgctcga tgggcgcgat ggtcaatgat cgccaagctt
     3181 cgatcgtggt ctcgcagaac acccgcgctc cgctgatccc ggtctcggtg aagattcacg
     3241 gcatgcccgg gttgcccagc gcgaactgga acttcgagat tgcgcacgag cgcttcttgt
     3301 cgccttcgtt cgtcgccgta tccctcggta gcgcgctgca agccgtggcc aacgaacacc
     3361 aagacgtgac ctggaccgcg cggagcaaag tgcaattgcg ggggcgcgcg gcgatcgagg
     3421 tcgaggacta tgggctctcc atgggcggta cccccgaccc cggtgagctc gggcgttcga
     3481 acctggtgcg cgctgtcggc tcgatcctga ataatccgtg ggagcccacc ttcgtggacg
     3541 gcatccaggt cgacttggag ctgagctatg cgcgtgatgt gctgcgtctg aggggcgccg
     3601 agctgctcga gccggaagtc gaggcggggc agcccgcgcg tataaagctc acgttcgtcc
     3661 cgttctcggg gcccgcgcag acgcgcctcg tctccgtgcc gctgcccgcg catttggcgg
     3721 ggcagaccgt gaacatggaa ctgctgcccg gatacatggc ggatcgcgaa caggctgcgc
     3781 ccgacagctt ggacgagcta attcacaatc tggagcaccc cacgtacccg ccgaagtctt
     3841 tggtggtgga gttctcggcc ggcagcggcg ccgtcgcgtt ccgcgggcga gtcgcgaagc
     3901 atctcccgcc gtccgctttg gatgcgctgc ggccgacctc gagcacggtg gctcccgacg
     3961 cgttccaaac ctcgaaccgc gaggtggtgt tgttgccgaa gttcgtgatg ggtcgtgatc
     4021 ggatcagcgt ttcggtgcgg ccggtgttgc gttgaggcgg gccatgggtc ttggttgcat
     4081 gagcgtaggt gaacggttga acatgcgttt ccttttcgga ttcaggcttc aggggctcgg
     4141 cgcagtcgca ggagggcttt cccgcgtcgg ttcggcacag ggcagggccc gcgtgggcgc
     4201 ttgcgcgctc gtgctcgcga cttgcgcttg gcacgctccc gcccacgccg tgggcacacg
     4261 gcacttcgtg ctcgacaagg gcggcgattt caaaggcgga gatctgaagg gcgtcgcggt
     4321 ggacacggcc ggtggcgtca gagcgggctt caatctcggc gcggctccga tgccggatgc
     4381 ggccacgatt tgggccgctt tgccgcaaaa agacggctca ctgttggtgg ccacgggcaa
     4441 tgacggcaag ttgctgcgcc tggctcaggg agcgagcagc gtcgtcgccg aaaccaaagc
     4501 tctcgccgcc acgtcactcg cggacgcttg ggttggcacc gtcatcgtcg gaactttgcc
     4561 cgagggcaag gtgatgaagc tcgatcgcgg tcgtctgacg gagctcgtac gcctaaaggg
     4621 cgcggagcac gtgtggcagg tcgccttcga tcccaaggct caggtggcgt atgccgcgac
     4681 c
//
LOCUS       DASTKU010000779         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_375891, whole genome shotgun sequence.
ACCESSION   DASTKU010000779 DASTKU010000000
VERSION     DASTKU010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375891"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFQ61_27230"
     CDS             complement(<1..891)
                     /locus_tag="VFQ61_27230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958229.1"
                     /translation="MLRTFLGTLGSAAGILWYASSAASSAADPNTATPGAGAARCRGQ
                     ECEELLPSGYKFGPDEDVTPGKKTSSDIKSKAKNLLAKIGIGNHAPRWVGAAPADFTQ
                     TERSADERGVNPCWSPDPGFGDYGAWDYSPSIGQMLVPQKLELDADGKVPVMFHFHGH
                     EPARKEWVQALDSAVLVALDLGINSGPYLRKFERPETFEALVSSVEDALEKRLGEKLS
                     IGKIGVSSWSAGYGAVQRVLESPMASRVDTVILLDGLHTSFTNTEGARLTMAPFVRFA
                     QRAAAGETFMHVSHSSILPPG"
     gene            1205..>2085
                     /locus_tag="VFQ61_27235"
     CDS             1205..>2085
                     /locus_tag="VFQ61_27235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958230.1"
                     /translation="MRAAQLQWALLTVAVVGCRTSPASERTDREAPSVAAENARVTTT
                     TVVPETTSTSVTEAPSKVEPSAGRSLAFRETYDATRTAPIEEQQRYLVELLSHDMQLT
                     PEQRDAVRAIMAKSEWLGFGNPEVSKPAMTKAECRQRRSAGSWRAGDPECKAPNMVAI
                     ETRDGRPRVCIDQYEFPNVACEYPVVWVRATEAAQLCEAVGKRLCDAHEWEGACAGEV
                     LSVEAEYPWDHMPKSIAHSPRREQRLWLEYEHNRTRDVRWAYGEHVDHALCGTGAKKE
                     PKCEVLDFSTCSTSSYPA"
BASE COUNT          395 a          647 c          678 g          365 t
ORIGIN      
        1 gccaggcggc aggatcgacg aatggctgac gtgcatgaac gtctcaccgg ctgccgcgcg
       61 ctgtgcgaac cgcacgaacg gggccatcgt caagcgagcg ccctcggtgt tcgtgaagct
      121 cgtgtgtagg ccgtcgagca agatcaccgt gtcgacgcgg ctggccatgg ggctctcgag
      181 cacgcgttgc acggcgccgt agccggcgct ccacgacgag acaccgatct tgccgatcga
      241 gagcttctcg ccgaggcgct tctcgagtgc gtcttccacg ctcgaaacca acgcttcgaa
      301 ggtctcgggt cgttcgaact tgcgcaggta cgggcccgag ttgatcccca agtccaacgc
      361 gacgagcacc gcgctgtcca gcgcctgcac ccactccttg cgcgccggct cgtggccgtg
      421 gaagtggaac atgaccggca ccttcccgtc ggcgtccagc tccagtttct gaggcaccaa
      481 catctgaccg atggaggggc tgtagtccca agcgccgtaa tcgccgaagc cggggtcggg
      541 tgaccaacac gggttaacac cccgttcgtc ggcggaccgc tcggtctggg tgaagtccgc
      601 gggggcggct cctacccaac gtggggcgtg attgccgatg ccgatcttcg cgagcagatt
      661 cttcgccttg gattttatgt cgctggaagt tttctttcca ggtgtaacgt cttcgtcggg
      721 gccgaacttg tacccgctgg gtaggagctc ctcgcactcc tggccacgac aacgggcggc
      781 gcctgcaccc ggggttgctg tgttggggtc tgccgcgcta gaagctgctg agcttgcata
      841 ccagagaata cccgcggctg atcccagcgt accgaggaat gtacgaagca tgtagtccgg
      901 gactctgcgc cgacctcatc gattgcgcaa gaggagttgc atgacaaatg gcctgtgggg
      961 tcgaattgta cgcgcgcaat agtcctgtaa tcttcgatac tgttcgaaag tggcgttctt
     1021 tttgcgccaa cactgatctt gacagacgct tgttcgagcc tgggacatct gacgtgcgga
     1081 tactcgaacg ttcagtgtta gccagtgtga gacaacgcgc cacgtcgggc ccggtcgcgt
     1141 gcactaatgg cggccagtgg ttaactaatc ggccgggcgc ccttcgaacg acgggggacg
     1201 agaagtgagg gcggcgcaac tccaatgggc gctgctcacc gtggcggtcg tcgggtgtcg
     1261 tacctccccc gcgagcgagc gcactgaccg cgaagcgccg agcgtcgccg ccgaaaatgc
     1321 ccgagtcacc accacgacgg tcgtccccga aaccacgagc acatcggtga ccgaggcgcc
     1381 gtcgaaggtc gagccgagcg cggggcgtag cctggcgttt cgggagacgt acgacgcgac
     1441 ccgcaccgca ccgatagagg agcagcaacg gtatttggtc gagctattgt ctcacgacat
     1501 gcagctcacg cccgagcagc gagacgcggt gcgcgccatc atggccaaat cggaatggct
     1561 gggcttcggc aatcccgagg tcagcaagcc cgcgatgacc aaggcggagt gccgtcagcg
     1621 ccgcagcgcc ggaagttggc gcgcgggaga ccccgagtgc aaagctccca acatggtggc
     1681 catcgagacc cgtgacggta ggccgcgggt ttgcatcgac caatacgagt tcccgaacgt
     1741 cgcctgtgaa tacccagtgg tttgggtccg cgcgaccgag gccgcccaac tgtgtgaagc
     1801 agttggcaaa cgtttgtgtg acgcacacga gtgggagggg gcgtgcgcgg gcgaagtgtt
     1861 gtccgtcgaa gcggaatacc cttgggacca catgcccaag tcgatcgccc acagtccacg
     1921 ccgcgaacaa cgactgtggc tcgagtacga acacaatcgc acgcgcgacg tgcgttgggc
     1981 ttacggagaa cacgtcgacc acgccttgtg cggcaccggc gccaagaagg agcccaagtg
     2041 cgaggtgctc gacttcagca cgtgcagcac gagctcctac cctgc
//
LOCUS       DASTKU010000780         5976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1270390, whole genome shotgun sequence.
ACCESSION   DASTKU010000780 DASTKU010000000
VERSION     DASTKU010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270390"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFQ61_27240"
     CDS             <1..588
                     /locus_tag="VFQ61_27240"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HET9958231.1"
                     /translation="ADVMLMMDSVTRFAMALREAALAAGEPPATKGYPPSVFAALPRL
                     LERAGTSGGAGVITALYTVLVEGDDLSDPIADTVRGILDGHIVLSRSLAEKNHFPAID
                     VLSSISRLAPEIAPEAQLKAAGAIRDSMSAYRDALDLIQVGAYAAGSDPRVDQACAVQ
                     PHVDQFLRQKTTERTSLEETQARLLKLAMAASPRR"
     gene            662..1126
                     /gene="fliJ"
                     /locus_tag="VFQ61_27245"
     CDS             662..1126
                     /gene="fliJ"
                     /locus_tag="VFQ61_27245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HET9958232.1"
                     /translation="MTQRKKRLDKLKEHRARAVDERLSDLSKVRALEQAASQRAEREA
                     SSLERARQQREQAFSGATDVSRLSDATDWLLACAARKDAALQELTRAQRAVARAQQEV
                     LAARNELRKIELVAERLALEERKREERVEQKMTDEFAALRFREPKKSGEDER"
     gene            1123..1785
                     /locus_tag="VFQ61_27250"
     CDS             1123..1785
                     /locus_tag="VFQ61_27250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958233.1"
                     /translation="MSQAGSMGGSGRIPEHAGFARESEAKANQQQAERWAQVLLPYVA
                     QVLPPAAPETTAPAGSPTTWRVGDGALTLARGVPLTAGSVTDPTQHDSPDDSLNRVHV
                     TVRTPEMGDLSLVLERGEAGLRVVIGVENSNLGQQLLPEQRALVQSLVGSGVKLQSLS
                     IVNQSELGTVLAQTANATRGARPGNIRGGAAQAAFTDSDGEARSGTEKARRRSRKLDF
                     IG"
     gene            1790..2488
                     /locus_tag="VFQ61_27255"
     CDS             1790..2488
                     /locus_tag="VFQ61_27255"
                     /inference="COORDINATES: protein motif:HMM:NF025230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HET9958234.1"
                     /translation="MVSAINSTTAAQGTAADMLSQGLTGDKALGRDAFLKLLVAQLRH
                     QDPLKPQDDSAFVAQLAQFSSLEQTMGINDRLDTLSMQSQGLQNSQVVGLVGKQATVR
                     GNVVTTDGSGGGVPLAFTLDGAAEKSTVTVRDSNGNVVRTLEVGKRSAGVVRLTWDGR
                     NDAGTVQPEGSYSIAVSAANEAGGAVSVSQSTKGTVQSVSFDKGYPVLHLDSGVNVPV
                     SDLLQVDSPPSPSK"
     gene            2525..3823
                     /locus_tag="VFQ61_27260"
     CDS             2525..3823
                     /locus_tag="VFQ61_27260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HET9958235.1"
                     /translation="MVLRAMYSGVSGLRAEGEALGVVGDNIANVNTVGFKSQRAIFQD
                     VLGHSILAGTASALPGSGVRVGKIQQMFTQGTLSNTGVATDLALNGDGFFVVNGQVDG
                     IAGNFYTRAGQFVLDKDGFLTNPQGLRAQGYAANNDGTFDAAVTSVQAPTASLPARQT
                     AAVSVTANLDAAAKVPTAAFDAQDPVNTSNFSTTMTVYDSLGNGHTMDVYFVKTADNA
                     WDYHALVPGDDLNPPQAGLNVEVGTGSLAFTSGGALDNMTATTPITVDFGGGAATGQA
                     ITLDFGTPIAAGGTGLEGTTQFASASSVSSQSQDGYSAGDFSGISVDGQGVVMGLYTN
                     GQKLAISQLAVAKFRSNDGLGRAGQSLWIETRDSGTAAMGTAASGGRAAVSSGAIEGS
                     NVDLAEEFVGLIQHQRSFSANSKTITTADEMLQELINIKR"
     gene            3854..4426
                     /locus_tag="VFQ61_27265"
     CDS             3854..4426
                     /locus_tag="VFQ61_27265"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HET9958236.1"
                     /translation="MSATPEDAHAAAPKKGKGKMVGIIVALVLVLVAGGAGAVLAPRL
                     LAKNNAAEKPKTEDSAEAAEPEEKHEEPAEEEEEEEPKKPSAAGAIGEVAEISPIVVD
                     TRAQDGSIRHVKVQFAIEIKHGVKPEDVKKLIPFAREAAIAYLRTQEFERLSDPKTFD
                     IVRKELSTEVVRAVGKARAKRILIVDFVAQ"
     gene            4536..5456
                     /locus_tag="VFQ61_27270"
     CDS             4536..5456
                     /locus_tag="VFQ61_27270"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HET9958237.1"
                     /translation="MAQSNALEEDRVEPAQAPADSQTLKRLDLTGRERRLRSAMQAVA
                     RVAQNFARGARRSMPFLVKRKSRLTPASVAFVDLAPDALMVDGPSFEVLLEEPDGPGR
                     ACLFLNTDALAVVLEGALGGAGDAASGALGPALTVAQTALVSKVVRQLGEDLVRAVRD
                     EVGLTLKVTLAHAIAAGEEREAAFSDGLGVDCGVEGLSPSARISLSMAAEALEVALKE
                     RDSEEVELGDPRIAEALHDVSVEVVAELGRVSIGLKRLLTLQPGQVLRLMTAVEDPAK
                     IRVAGVTKFVGSPVVSRGQLAIQIKARHED"
     gene            5470..5829
                     /gene="fliN"
                     /locus_tag="VFQ61_27275"
     CDS             5470..5829
                     /gene="fliN"
                     /locus_tag="VFQ61_27275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HET9958238.1"
                     /translation="MTDPSNPERGSFPPGRDKPSKLDASSFAPPLRGADSLAFVMDVP
                     VELTIELGRKNVKIGEVLKLGPGSVLELDKTNGDPLDVYVNNRLVARGEAVVVGERYG
                     IRLIEVLVGDEASGSKP"
BASE COUNT         1121 a         1845 c         1983 g         1027 t
ORIGIN      
        1 gcggacgtga tgctgatgat ggactcggtc acgcgcttcg cgatggcgct gcgtgaagct
       61 gcgctcgccg cgggtgagcc gcccgccacc aagggttacc cgccaagtgt gttcgcggcg
      121 ctgccgcgct tgctcgagcg cgcgggtaca tcgggcggag cgggcgtgat caccgcgctt
      181 tatacggtgc tcgtcgaagg cgacgatctt tcggatccga tcgcggacac cgttcgcggt
      241 atcctcgacg gtcacatcgt gctctcgcgc agcctcgcgg agaagaacca ctttccggcg
      301 attgatgtgc tgtccagcat ctcgcgtctg gctcccgaga tcgcgcccga agcccaactc
      361 aaagccgcgg gcgcgattcg tgactcgatg tcggcttacc gcgacgcgct cgatttgatt
      421 caggtcggcg cgtatgcggc gggcagtgat cctcgggtcg atcaggcgtg tgcggtgcag
      481 ccccacgtcg accagttcct gcggcaaaag accacagagc gtacgagctt ggaggagacc
      541 caggcacgcc tcttgaaact cgccatggcc gcgagtcctc gccgctgagc cgcccccgga
      601 ccacttcggt gggctggctc gtgcgcggag cttcgcgctc gcgtctgcct gaacagccca
      661 catgacccag cgcaagaaac gcctcgacaa gctcaaggaa catcgggcgc gcgcggtcga
      721 cgagcggctg agcgacttgt ccaaggtgcg ggctttggag caggccgcct cacaacgcgc
      781 cgaacgcgaa gcttccagtt tggagcgagc tcgtcagcag cgcgagcagg cgttctccgg
      841 tgccacggac gtgagtcgtc tcagtgatgc caccgattgg ctgttggctt gcgccgcccg
      901 caaagacgcc gccctgcagg agttgacgcg ggcgcaacgc gcggtggcgc gggcccaaca
      961 agaggtgctc gcggcccgca acgaactacg gaagatcgag ctggtcgcag agcgcctcgc
     1021 gctcgaggaa cgcaagcgcg aggaacgcgt cgagcagaag atgacggacg agttcgcggc
     1081 gttgcgcttt cgtgaaccca agaaatcagg agaagacgag cgatgagtca ggcggggtcg
     1141 atgggcggtt cgggtcgcat tccggagcat gcagggttcg cgcgcgagag cgaggcaaag
     1201 gcgaatcagc agcaggcgga acgctgggcg caggtgttgc tgccctacgt cgcccaggtg
     1261 ctcccacccg cggctccgga gacgaccgcg ccggcaggct cgccgaccac ttggcgcgtg
     1321 ggtgacggag ctctgacgct agcgcgtggc gtccctttga cggcgggcag cgtcaccgat
     1381 ccgacgcagc acgattcgcc cgatgactct ctcaaccgag tccacgtcac ggttcgcacg
     1441 cccgagatgg gcgacctctc gctcgtgctc gaacggggtg aagccgggct gcgcgtggtc
     1501 attggggtcg agaactcgaa cctcggccag cagctgcttc ccgaacaacg cgcgctcgtt
     1561 cagagcttgg tgggcagcgg cgtcaagctc cagtcgctca gcatcgtgaa ccaatccgag
     1621 ctcggcaccg ttcttgctca aactgccaac gcaacccgag gcgcacgacc cggcaacatt
     1681 cgaggaggag cagctcaagc tgcttttacc gacagtgacg gggaggcgcg atcgggcacc
     1741 gagaaggctc ggcgcagatc tcgcaaactc gacttcatcg gataatcaca tggtatcggc
     1801 gatcaactcc acgacagcgg cccagggcac ggcggcagat atgctgagcc agggtttgac
     1861 gggagacaaa gcgctcggtc gcgatgcctt cttgaagttg ctggtggcgc agctccgaca
     1921 ccaggacccg ctcaagcccc aagatgatag cgcgttcgtc gcgcagctcg cgcagttctc
     1981 ctcgctcgag cagacgatgg gcatcaacga tcgcctggat acgctctcca tgcagagcca
     2041 gggcctgcaa aactctcagg tcgtgggcct ggtgggcaag caggcgacgg tgcgcggaaa
     2101 cgtcgtgacg accgacggct cgggcggcgg cgtgccgctt gctttcacgc tcgacggtgc
     2161 cgccgagaag tcgacggtta ccgtgcgcga ctcgaacggc aatgtcgtgc gaactctcga
     2221 agtaggcaaa cgctccgccg gtgtggtgcg cttgacctgg gacggtcgca acgacgcagg
     2281 caccgtgcag ccggaaggca gctacagcat cgccgtgagc gcggcgaacg aagcgggtgg
     2341 cgccgtctcc gtctctcaat ctacgaaggg cacggtgcaa tccgtgtcgt tcgacaaagg
     2401 ctatccggtc ctccacctcg attctggggt gaacgtgccg gtctccgacc tcctccaggt
     2461 cgattctccg cccagcccca gcaagtaacc ctcaactcag gatccgattt ttcgggagga
     2521 ctcaatggtt ctcagagcaa tgtattcagg tgtatccggt cttcgtgccg agggggaagc
     2581 cctcggagtg gtcggcgaca acatagcgaa cgtgaacacc gtaggcttca agagccaacg
     2641 agcaatcttt caggacgtct tggggcactc gatcctcgcc ggtacggctt cggcgttgcc
     2701 gggttcgggc gtgcgcgtgg ggaagattca gcagatgttc acgcaaggca cgctgtcgaa
     2761 cacgggcgtc gccacggacc tcgcgctgaa tggcgacggc ttcttcgtcg tcaacgggca
     2821 ggtggacggt atcgcgggta acttctacac gcgcgccggt cagttcgtgc tcgacaagga
     2881 cggcttcctg acgaacccgc agggcttgcg tgcccagggt tatgccgcca acaacgacgg
     2941 caccttcgac gccgcggtga cctcggtgca agcgccgacg gcctctttgc cggcgcgcca
     3001 gaccgccgcc gtctccgtga ccgccaacct ggatgcagcg gccaaggtgc ctaccgcagc
     3061 tttcgacgcc caagatccgg tcaatacgtc gaacttctcc accacgatga cggtctatga
     3121 ctcgctcggc aacgggcaca ccatggacgt ctacttcgtg aagacggcgg acaacgcctg
     3181 ggattaccac gcgctcgtcc ccggtgacga tctgaacccg ccccaagctg gcttgaacgt
     3241 ggaagtgggc accggttcgc tggcgttcac ctcgggcggc gcgctcgaca acatgaccgc
     3301 gaccacgccg atcaccgtag acttcggcgg gggtgccgca acgggccagg ccattacttt
     3361 ggacttcggc acgcccatcg cggcgggcgg aaccgggctc gaaggcacca cccaattcgc
     3421 ctccgcgtcc agcgtgtcga gccagtcgca ggacggctat tctgccggtg atttctcggg
     3481 catttccgtc gatggtcagg gcgtcgtgat ggggctctac accaatggac agaagctcgc
     3541 gatctcgcag ctcgcggtgg cgaagttccg ctccaacgac ggtctcggcc gcgctggtca
     3601 gagcctctgg atcgagacac gcgacagcgg cactgcagct atgggcaccg ccgcgagcgg
     3661 tgggcgtgcg gctgtgtcct cgggcgccat cgaaggctcc aacgtcgatc tcgcggagga
     3721 gttcgtcgga ctcatccaac atcaacgctc cttttcagcc aactccaaga cgatcaccac
     3781 cgccgacgag atgctgcaag agctcatcaa catcaagcgc tgagcgtagc gtgagtaccc
     3841 cctcggagat gtgatgagcg cgaccccgga agatgcccac gccgctgccc ccaagaaggg
     3901 caaaggcaag atggttggca tcatcgtagc cctggtgctg gtcttggttg ctggcggcgc
     3961 aggtgccgta ctggcgccgc gcttgttggc caagaacaac gcggcggaga agcccaaaac
     4021 ggaggactcc gccgaagccg ccgagccaga ggagaagcac gaagagcccg cggaggaaga
     4081 ggaggaggaa gagcccaaaa agccgagcgc agctggggca atcggtgaag tggctgaaat
     4141 ctcccccatc gtcgtggaca cgcgtgctca agacgggtcg atccgccacg tcaaggtgca
     4201 gttcgcgatc gagatcaagc acggcgtcaa gccggaggac gtgaagaagc tgatcccgtt
     4261 cgcgcgcgaa gccgccatcg cctatctgag gactcaagag ttcgagcgct tgtcggatcc
     4321 gaagaccttc gacatcgttc gcaaggagct ctcgaccgag gtcgtgcgag ctgtcggcaa
     4381 ggcacgagca aaacgcatct tgattgtaga cttcgtggca caatagccac ccgagagatt
     4441 ctcgcaccat atcgtctgcg caccgcaagc tagctcgacc cgtgggtggc tagcgctggc
     4501 gcaccgagag cacagaatag gaatcgagga cacctttggc tcagtcgaac gcgctcgagg
     4561 aagatcgagt ggagccggca caggcacctg ctgattccca aactctgaag cgtttggatc
     4621 tcacggggcg cgagcggcgc ctgcgcagcg ccatgcaggc cgtggcgcgg gtcgcgcaaa
     4681 atttcgcgcg cggcgcgcgg cgaagcatgc cgtttctggt caagcgcaag tctcggctca
     4741 cgccggcgtc cgtggcgttc gtggatctcg ctcccgacgc gttgatggtc gatggcccct
     4801 ctttcgaagt gctgctcgag gaacccgatg ggcctgggcg ggcttgcctg ttcttgaaca
     4861 cggacgcgct ggcggtcgtg ctcgaagggg cgctcggcgg agcaggggac gcggcgagtg
     4921 gggctttggg cccggcgctg acggtggctc agaccgcgct cgtctccaaa gtcgttcggc
     4981 aactcggtga ggacttggtg cgtgcggttc gggatgaggt gggcctcacc ctgaaggtta
     5041 cattggctca cgccatcgcc gcgggtgagg agcgtgaagc cgcgttctcc gacggcttgg
     5101 gcgtggactg tggcgttgaa gggctgtcgc cctccgcacg gatttcgttg tcgatggccg
     5161 ccgaagcgct ggaggtggct ctcaaggagc gtgactccga ggaagtcgag ctgggcgatc
     5221 cgcgcatcgc ggaggcgttg cacgacgtct cggtcgaggt cgtggccgag ctcggccgcg
     5281 tatccatcgg tttgaagcgg ctacttaccc tgcaacctgg ccaagtattg cgcttgatga
     5341 ccgcagtaga agatcccgct aaaattcgcg tcgcgggcgt aacgaagttc gtgggttcac
     5401 ccgtcgtctc gcgcggacaa ctcgcgattc aaatcaaggc gcggcacgaa gattgaaagc
     5461 ctctcgggta tgaccgaccc gagtaacccg gaaagaggat cgttcccgcc gggccgagac
     5521 aagccttcca agctggacgc ttcatcgttc gcgcccccgc tgcgtggtgc cgattcattg
     5581 gccttcgtga tggacgtgcc ggtggagctc accatcgagc tagggcggaa gaatgtcaaa
     5641 attggcgagg ttttgaagct tggcccggga tccgtactcg agctggacaa gaccaatggg
     5701 gacccgctcg atgtgtacgt gaacaatcgt ttggtcgccc gaggtgaagc ggtcgtggtc
     5761 ggtgagcgtt acggtatccg gctcatcgag gtactagtcg gagacgaggc atcggggtca
     5821 aagccatgac gttgccgcgt cagggaaagc gcggggcaat accgctcaag tatcgttccg
     5881 tggccgcagt gttggcgctt ggcttggcct tgagtgcttc ggcacgcgca caggaaagcg
     5941 aatcgactac ctcgccgccc gcggcacagg aagatt
//
LOCUS       DASTKU010000781        16066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1716898, whole genome shotgun sequence.
ACCESSION   DASTKU010000781 DASTKU010000000
VERSION     DASTKU010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16066
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716898"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFQ61_27280"
     CDS             complement(<1..377)
                     /locus_tag="VFQ61_27280"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9958239.1"
                     /translation="MTNPISIGDPASSRNGGWTRGRVIFLSISAVLVVTALMWAREVL
                     LPFILAIIIAYVLTPLVERCERLRVPRAAAILLVYAITLGSLYGFGALAAGRIAAEGR
                     ALVREVPKLLKHLQDEVIPEWEG"
     gene            complement(409..2151)
                     /locus_tag="VFQ61_27285"
     CDS             complement(409..2151)
                     /locus_tag="VFQ61_27285"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET9958240.1"
                     /translation="MTDTQTATHDKNPLSGFAALFEASEEKGDIGGEGEIVAGVVVQV
                     TRDSVVVDIGGKSEGVIARDEFLDAHGALTVKAGDRVDVFVESRESDDGLISLSKEKA
                     DKMKVWDEISSACERDEIIEGTISQRVKGGLSVTIRGGVKAFLPGSQVDLRPIRNLEK
                     LIGQTYEFKVIKFNKKRGNIVLSRRALLERERDEMKAKTLQTLEEGMVVTGTIKNLTE
                     YGAFVDLGGIDGLLHITDMSWGRVNHPGEVFKVGDEVTVKVLKYNADTERVSLGLKQT
                     QEDPWNHAEEAYPVGKRVRGKVMSLTDYGAFIELEPGVEGLIHVSEMSWTKKIKHPSK
                     LLEMNQEVECQVLEVDAKSKRISLGLKQLEPDPWTLFTDKYKPGDKISGKVRSITDYG
                     VFVGIEEGVDGMVHKTDLSWTVKINNPADVYGKGDDVEAIILSINHDEKKVSLGVKQL
                     WDDPWLDSMSRYKPGTVVEDALVLSVTDYGAFVRVVEGIEGLIPTSEIPPTATISRGD
                     KVKAEISNVDGVDRRVTLTMRNPGESQEAEQLMVMRRESQGKGATLGDVLKEKLESMK
                     KTPSVAPPPNDSDK"
     gene            complement(2342..3331)
                     /locus_tag="VFQ61_27290"
     CDS             complement(2342..3331)
                     /locus_tag="VFQ61_27290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958241.1"
                     /translation="MTGLDPNAAVSSAEPIVRPSATRSVSFRAVAVVFLGLLVLGLGL
                     AVWVHKRYVAYQPTAARHVASDAVVALRLDLTHVMFYEPFRRALLPLVDLGPSGAHRA
                     ERLAERGLRASGDVREIVVASRAELGSLSVVLGGTFERRGVTRKLAEVLRGDGIEVLA
                     SSGELEAFCMTGGPCFAQFDDGSIAFAQHEQELLAVRAATAAPPALVNAAGGFFLRGR
                     EIERMQNRVAQAVGGSTFRADPDWASVERLELNFRAGSVVELQLKASLTDESRGRAAI
                     ERTLAELAHDAPEFDSQKAQLRLEGRELSGRFRLPRESVERLFSRLAEVLKLL"
     gene            complement(3419..4237)
                     /locus_tag="VFQ61_27295"
     CDS             complement(3419..4237)
                     /locus_tag="VFQ61_27295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958242.1"
                     /translation="MPADLDLVVRVDLERLRGSISPELFGALEKRISESSSGQDDEPT
                     ARWLLQALGRADTAWLAVRPGLAAELTDSVVVLRGRFADSQGELLAPPWGHGRDLGGT
                     VLRFERAAPKLRASPALVVLRPPDRLLISTSAEIDALDRSLEGRGEEPLRAPESGLVS
                     GAARLQLLKERLADRVPRVAKLLEGAAHARAKLDRTGERYPLELELTFETPQQAAEFA
                     QAAERVRAALVDPSRAWLGQLQIRAFEAYVSLNVELSERDLLRAVACWIGSCTP"
     gene            complement(4380..5087)
                     /gene="cmk"
                     /locus_tag="VFQ61_27300"
     CDS             complement(4380..5087)
                     /gene="cmk"
                     /locus_tag="VFQ61_27300"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET9958243.1"
                     /translation="MISRNRPVVAIDGPAGAGKSTVTRLVAARLGYWLLDTGALYRAV
                     ALAVVRAGIDWADTERISTFASELVRREGLIVERGHDGEQLVRLDGEEVQGLLRSQEI
                     GAGASRISSLPRVREALLALQRRAAERGGVVVEGRDIGTVVFPDAEAKFFLTASVEVR
                     ARRRFDELAARGAAPSLEDVRREVVERDLRDTERAVAPLRRAQDAELVDSSELDLEAV
                     VGRIVERVHSVERRLKG"
     gene            complement(5084..6427)
                     /locus_tag="VFQ61_27305"
     CDS             complement(5084..6427)
                     /locus_tag="VFQ61_27305"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET9958244.1"
                     /translation="MSRVVIRPSPRPLHGSVPVPSDRSITHRALVLAALANAPSILHG
                     FGYGKDNLATLNALHALGVRTEDDGRGTLRVRGVGLAGLNAATEPLDCGHSGTALRLL
                     TGVLAGQPFESRLVGAPRLERVDLGATLEPLWRRGARIESREESAQHFAPLHLLGVEP
                     GTRLAPVEYALTSANDHAKGALLLSGLFASGPTTVHENVVARDHTERMLVAMGVPIET
                     LGSVLRLHPPRDPLSLRAFVCDLPGDLSAAAFLLVAAQLVPDSNVSTRRTGLNPTRSA
                     ILDIIRMFGGRMGITPGGDTLGEPFGEVNSRGSVLRASPVTGELAERALDELPIACAL
                     AARARGVTEFSNMGVLRADQPDRVHALAQVLAAFGIAVDEREDGLLIEGQCDRPLRAS
                     RVDSRGDHRIAMLAAVLGLVADGESWVDDADCIAVSFPRFVGTLRALGADLEVVS"
     gene            complement(6433..7419)
                     /locus_tag="VFQ61_27310"
     CDS             complement(6433..7419)
                     /locus_tag="VFQ61_27310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958245.1"
                     /translation="MTRPSRRQLLEWGIGFGLVSAGCGKQGDVIVESGAGKGLGGHAI
                     DADPLALLPPNAVGAATLDARALFASRFGARLFALTQAKSPLPASAEFDPRRDLERVY
                     VGFYSMQGADVAGVATGTFQPEKIEAAATSNPQTFGGAKLTLQEYAGRNMYLTDSLGF
                     CVLTSRTVLFGDETGMRRALDRISEGRAQRKLPAWMVDVLERPKAPLSGGADFTSQPL
                     SAAAREQVPFLNGVKTLAVVGNFQEPGLNLAGTLTYDEPEAALRGAENLRKMHERLAN
                     YAAILSIIGLPQPVRSLEARAEDKEVRFVAGVDGAAVAVLLEKAEAYLAQKR"
     gene            complement(7416..8561)
                     /gene="hisC"
                     /locus_tag="VFQ61_27315"
     CDS             complement(7416..8561)
                     /gene="hisC"
                     /locus_tag="VFQ61_27315"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET9958246.1"
                     /translation="MTNRSAEPISGPITGAAPAREASSLVLPSIEALVPYETGKPIEE
                     LARELGISDAVKLASNENPLGPSPKAIAAVREVLERIHRYPDGAAFTLREKIAARLGV
                     SMPEVIQGNGSNELLDLMVRTFATPSDHIVFAEPSFVVYRIAAMSGGIPFTAVPLRDQ
                     RHDLESMADAIRPETRLIFVANPNNPTGTHVGAGELERFLRRVPQHIVVVLDEAYVEY
                     ADAPDFVDGLRLRGLRERLLVVRTFSKIYGLAGLRVGYAVGPAKLVDYLNRVRAPFNV
                     SSPGQAAAIAALDDREHVTRSRDLNARERARLSAELTRLGCKVTPSQANFLWIELSVP
                     AAGVYDALLRKGVIVRPFRTWPTFLRITVGTEAENDRMLRALTEVLS"
     gene            complement(8727..9797)
                     /locus_tag="VFQ61_27320"
     CDS             complement(8727..9797)
                     /locus_tag="VFQ61_27320"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HET9958247.1"
                     /translation="MSDRRREVQELRENVARVDAEICKSLDARASLSKKIHGLLEAEP
                     SADGSEGEWLEKVLANSTGEMPAESVRVIFRAIRAAGRAIEQPVRIAVVGPEGGFCHQ
                     STLEAFGATTQTIECATAQEALAEVTRSRAAFAVFPFESSAEGLQQPTLLSLADSDLV
                     LVAERFLPSSYDLMSTGDEMSGVERVYMTAFAHAACQRFLDAELPRATIIDVRTPKDA
                     ADLAREAKSSAAVVPERCGRHAGLERLRENVGDLPDLRIRYGVAGQRPAARTGRDISC
                     LLFSVENEPGSLYEVLRHFSERGINLRKLQSIPARRDGFEYYFYAEITGHASDRPVVT
                     AFESVKRSVRYFRLLGSFPSAS"
     gene            10099..10278
                     /locus_tag="VFQ61_27325"
     CDS             10099..10278
                     /locus_tag="VFQ61_27325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958248.1"
                     /translation="MAVELLEEVLEPEELPELELPDVLEPLEELEVPELLDPELLDPE
                     PLDPELLEPPGLAEL"
     gene            11168..14815
                     /gene="smc"
                     /locus_tag="VFQ61_27330"
     CDS             11168..14815
                     /gene="smc"
                     /locus_tag="VFQ61_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET9958249.1"
                     /translation="MHIKKLEIAGFKSFVDRTAIHFDNDVIGIVGPNGCGKSNIVDAI
                     RWCLGEQSAKHLRGRAMEDVIFNGSESRGAAGMAEVTLTFDNTNPEYAASLPLEYRDY
                     SEIAVTRRLFRDGTSEYLVNKTQVRLRDVTDLFLGTGVGTKAYSIVEQGRIGQIVSAR
                     PEDRRLFIEEAAGITKYKQRRKQAERKMDLTRQNLLRINDIVAEIDRNRAALKRQVAK
                     AERFLEYRRELDDLVLHEAAHRLLELIVVERVERDAYTEAAQASGGLRDRVREAEAAI
                     DAARLEAAEVEALTEDASKRAFEADNQVSTLHAEIERARDRLKHLSDKLEAGRAELGE
                     LGGRQSGLASELELLRQKVNELARDERAREADALSETESLSELQGEEARASADVLGLR
                     RQSGELSAQAAALEAKVDGILNRVADARARRDRLVQEQDELNGELNTGLARQNALEKS
                     VQELAQGKHTTQVEREALARESESLRGRQLESERAVDVIKNEIGLKRNRLRALQDLHR
                     RLEGVGAGARALLSKGDPRVLGLLADRVEAPSELTQAVAGLLGNRLQTIVVSDAAAGL
                     ELLQELAREERGRASVISVRPSYVAGLQHSVGVDPRVRGRLVDRLSYAPSDEGLVRAL
                     IGDALLVESAGDAADVARVYPGVTTVALDGTVVHPDGVISGGDGDDVASAMLEQKREM
                     HELADDLSRLEAQGERLVADHNAIRARATEVSTALDRARQEAHEGELAHVTAEKDLAQ
                     TAAEITRFTARLGSIATELAEIDHMLAAAEASEGDSRSQLDGMRAELEHVGHALVKAE
                     DNASVWRERVAAQASLVTERRVRLAQVKEQLDAARQAHERVENSLLELESRGQRLHTE
                     LDEAAAVYGETAGRIVLARAARADAEAQAREAHATFEAARERLEQSRSTLSNQETDTR
                     RQRDELTTLEESARKHEMAMQRFELEREHLLTGIRERFRGLDLARVVGDYHARPPTDA
                     EHKRRIDELTQLIDRMGPVNLDAKAEHQDAERRFKELNDQKVDIEKALEELDQAIRHM
                     NRESRKRFRETFEEVNTLFKKTFSKLFRGGRGELQLTNPEDLLNSGVEIIAQPPGKKI
                     GSIELMSGGEKALTAVSLIFAIFQHRPSPFCILDEVDAPLDEANVNRYNEMVRAMTSH
                     SQFILITHIKSTMQSVDVLYGVTMAEPGVSRIVSVKVNEHAAARSGSSMTRAPAREGT
                     GSSEGTVAGVA"
     gene            complement(14887..>16066)
                     /locus_tag="VFQ61_27335"
     CDS             complement(14887..>16066)
                     /locus_tag="VFQ61_27335"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET9958250.1"
                     /translation="VMQIAIVGADYSGGEADQLRRDMAAWRRNGKLMRHRSRLLEGFE
                     QHGISREFGEALFEQIKGFGDYGFPESHASSFALLVYASAWQKAHYPAHFACALLNSQ
                     PMGFYSPATILGDARRHGVEVRDVDIAQSTWDTQLEPAGACSGIGPHATGAQRALRLG
                     FRLVKGLGEKAVRRIEAARSEWRFSDIQDVARRAELDRAEIDALAEAGAFESLVIGRR
                     QAAWLGRAPRLSGLFEKQSLKETPPRLPELRAAETLVLDYRRKRLSANDHPIRHLRPR
                     LAADGILCASELVDTSQSKPVKVAGVVICRQQPATASGVVFMTLEDETGFINLVLWRN
                     VFEQQRWVATQSAILMAEGKLERQVVPNTGSVIHLIVQRLERLDVPGRDIGKMSRDFH
                     "
BASE COUNT         3055 a         5213 c         4959 g         2839 t
ORIGIN      
        1 ttgccctccc actcgggaat gacctcgtcc tgcaggtgct tgaggagctt tggaacctcg
       61 cggacgagcg cgcgtccctc ggcggcgatg cggcccgcgg cgagcgcgcc gaacccgtac
      121 agcgagccga gcgtgatcgc gtagaccagc agaatggctg ccgcccgggg cacacgcagc
      181 cgctcacagc gctccacgag cggggtcagt acgtacgcaa tgatgatggc gaggatgaag
      241 ggcagaagca cttccctcgc ccacatcagc gcggtgacga ctaggaccgc gctgatggag
      301 agaaaaatga cgcgaccccg cgtccaaccg ccgttgcggc tagacgcggg atctccgatg
      361 ctgattggat tcgtcacgct gaagttcggt aaagccgaac tctaccgatc acttgtcaga
      421 atcgttcggc ggcggagcca ccgacggcgt cttcttcatg ctctcgagct tctccttgag
      481 cacgtcgccc aaggtggcgc ccttaccctg cgactcacgt cgcatgacca tgagctgctc
      541 cgcttcttgg ctttcgccgg gattgcgcat ggtgagcgtc actcggcggt cgacgccgtc
      601 gacgttcgag atttcggctt tcaccttgtc gccgcggctg atcgtcgcgg tcggaggaat
      661 ctcgctggtc gggatcagac cttcgatgcc ttcgacgaca cgaacgaatg ctccgtagtc
      721 ggtcacgctg aggacgagtg cgtcctcgac cacggtgccg ggcttgtagc ggctcatcga
      781 gtccagccac gggtcatccc acagctgctt gacgccgaga ctgaccttct tctcgtcgtg
      841 gttgatgctg aggatgatcg cctcgacatc gtcccccttg ccgtagacat cggcggggtt
      901 gttgatcttg acggtccacg acaagtcggt cttgtgaacc atgccgtcga cgccctcttc
      961 gatacccacg aacacaccgt agtcggtgat cgagcgtacc ttgccggaga tcttgtcgcc
     1021 gggcttgtac ttgtccgtga agagcgtcca gggatcgggc tcgagctgct tgagacccag
     1081 gctgatgcgc ttggacttcg catcgacctc gagcacctgg cactcgacct cttggttcat
     1141 ctcgagcagc ttggacgggt gcttgatctt cttcgtccag ctcatctcgc tgacgtggat
     1201 caggccctcg acgcccggtt cgagttcgat gaacgcgccg tagtcggtca gactcatcac
     1261 cttaccgcgg acgcgcttgc cgaccggata cgcctcttcg gcgtggttcc atggatcttc
     1321 ttgggtctgc ttcagaccga ggcttacgcg ctccgtgtcc gcgttgtact tgaggacctt
     1381 gacggtaacc tcgtcgccga ccttgaacac ctcaccgggg tgattgacgc gaccccagga
     1441 catgtccgtg atgtgaagca ggccatcgat accgccgaga tcgacgaacg cgccgtactc
     1501 ggtgaggttc ttgatggtgc cggtgacgac cataccctct tccagggtct gcagcgtctt
     1561 ggctttcatc tcgtcgcgct cgcgttcgag cagggcgcgt cgagagagca cgatattgcc
     1621 gcgcttcttg ttgaacttga tgaccttgaa ctcgtaggtc tgcccgatca gcttctcgag
     1681 gttccggatc gggcgcagat ccacctgcga gcccgggagg aacgctttga cgccgccccg
     1741 aatggtgacc gataggccgc ccttgacgcg ctggctgatg gtgccctcga tgatctcgtc
     1801 gcgttcgcag gcgctcgaga tctcgtccca caccttcatc ttgtcggctt tttccttcga
     1861 caaggagatg aggccgtcgt cactctcgcg gctctctacg aacacatcga cgcgatcacc
     1921 agccttgacc gtcaaggctc cgtgggcgtc caggaactcg tcacgggcaa tcacaccttc
     1981 gctcttgccc ccgatgtcca ccacgaccga atcgcgtgtg acttgcacga cgacgccggc
     2041 gacgatctcg ccctcgcctc cgatatctcc tttctcttcg ctggcctcga atagggcagc
     2101 gaaacccgaa agcgggtttt tgtcgtgcgt tgcggtctga gtgtcagtca tggaaagaat
     2161 ctttgcctcc agtcggggaa cctcttcggg atgctcggcc agcgctaagt ttgcgagcgg
     2221 ccacggcgtc gcctagggag gcggtctggt agcgcaccct gtccgccgag tcaaacctga
     2281 tcaccaagcg gagcacgatg gattcgattt agttcagtgg tgcagggaag aaatcgaggc
     2341 atcaaagcag cttcaggacc tcggccagcc gggagaacag gcgttccacg gactcgcgtg
     2401 gcagccgaaa acgcccggaa agttcacgac cttcgagtcg aagctgggct ttttgactgt
     2461 cgaactcggg ggcgtcgtgc gccaactccg cgagcgtgcg ctcaatcgcg gctcggcccc
     2521 ggctctcgtc ggtcaaactg gccttcaact gcagctcaac cacggacccc gcgcggaaat
     2581 tgagttccaa gcgctccacc gaagcccaat ccgggtcggc cctgaacgta cttccgccca
     2641 ctgcctgggc caccctgttt tgcatacgct cgatctcgcg gccgcggagg aagaagccgc
     2701 ccgccgcgtt gaccaaggca gggggcgccg ccgtcgcagc ccgcaccgcg agaagctcct
     2761 gttcgtgctg agcgaaagcg atcgaaccgt cgtcgaattg ggcgaagcac gggccgccgg
     2821 tcatacaaaa ggcctcgagc tctccggaac tcgccagcac ctcgatccca tcgccccgca
     2881 gaacttccgc taactttcgg gtcacccctc gccgctcgaa ggttccgccg agcaccacac
     2941 tcagcgaacc gagttcggcg cgcgacgcga cgacgatttc gcgaacatct cccgaggcgc
     3001 ggagcccgcg ctccgccaag cgctccgctc ggtgggcgcc agacgggccg agatccacca
     3061 gcggcaagag cgcgcgacgg aacggctcat aaaacataac gtgggtcaaa tccaggcgca
     3121 gcgcgacgac agcgtcggac gcgacgtgcc gcgccgcggt cggttgatac gcgacgtagc
     3181 gcttgtggac ccaaaccgcg agcccgagcc ccagcaccaa caagcccaag aacaccactg
     3241 cgaccgcgcg aaaggacacg cttctggtgg cagaaggacg gacgatgggt tcagcggagc
     3301 tcacggccgc attgggatcg agtccagtca tcttggcggt acctatactc ccccgcgagg
     3361 cgcttgcgga aacgtgctga aaggaaggca cctccggccg aaactttcgc gcgttacgtc
     3421 atggggtgca gctgcctatc cagcacgcta ctgcacgcaa caagtctcgt tcggagagct
     3481 cgacgttgag actcacgtag gcctcgaagg cccgaatctg cagctggcct agccaggcgc
     3541 ggctcggatc cacgagcgcc gcgcgtacgc gctcagcggc ctgcgcgaat tctgctgctt
     3601 gttgcggtgt ctcgaaggtc agctccaact cgagcggata gcgttcccca gtacggtcga
     3661 gcttggctcg tgcgtgggcg gccccttcga gcagcttcgc aacccgcgga actcggtccg
     3721 cgagtcgctc cttcaaaagc tgcaggcgcg ccgctccgga aaccagtcca ctttccgggg
     3781 cgcgcagggg ctcctcccct cgcccttcga gagatcgatc cagagcgtcg atctccgcgc
     3841 tcgtcgagat gaggagtcga tcgggcggac ggagcacgac caaggccgga gacgcgcgca
     3901 gcttgggtgc ggcccgctcg aaacgcagca ccgtgccgcc aaggtcgcgc ccgtgacccc
     3961 acgggggtgc caatagctcg ccttgcgagt ccgcaaagcg ccccctcaga acaacgacgc
     4021 tgtcggtgag ctccgccgcc aaccctggcc gcaccgcgag ccaggccgtg tcggcgcgcc
     4081 cgagggcttg caaaagccaa cgcgccgtgg gctcgtcatc ttgccctgaa gagctttcgc
     4141 tgatgcgctt ctcgagagcc ccgaacagtt cggggctgat gctgccacgg agtcgctcga
     4201 ggtcgacccg tacgacgaga tccaaatcgg cgggcacgct gagcgccggg tcatccagac
     4261 tcggtgccgg tggcggcgga gggctaggag ggggaccgca ggcactcaga caaaggcacg
     4321 caaggccgcc gcagcatgcg gccgcgagtc gccctatttt ccggcggcgc agccgcacgt
     4381 cagcccttca aacggcgttc gacgctgtgc actcgttcga cgattcttcc gaccacggcc
     4441 tcgagatcga gctcggagct gtcgaccaat tcggcgtctt gagcgcgccg taagggtgcc
     4501 accgcccgct cggtatcgcg caaatcccgc tccaccacct cgcgccgcac atcctctagg
     4561 cttggcgccg ctccccgcgc cgccagctcg tcgaagcggc ggcgcgcgcg aacctcgacg
     4621 ctcgcggtta gaaagaactt ggcctcggca tctggaaata ccaccgtacc gatgtcgcgc
     4681 ccttccacca cgaccccgcc gcgctcggcg gcacggcgct ggagggcgag caaggcttcg
     4741 cgaacccgcg gcaacgagga gatgcgactg gcgcccgcgc caatctcctg cgaccgcagc
     4801 aacccctgca cttcctctcc atccagccgc acgagctgct caccgtcgtg cccccgctcc
     4861 acaatcaagc cttcgcgccg cactagttct gaagcgaacg tggatatccg ttcggtatca
     4921 gcccagtcga tacctgcgcg caccacggcc aaggccaccg ctctgtacag ggcgccggtg
     4981 tccaacaacc aataaccgag ccgagcggcc acgagtcgcg tcaccgtcga cttacctgcg
     5041 ccggctggcc catcgatggc gaccacgggg cgattgcgcg aaatcacgag accacctcca
     5101 gatcggcgcc aagcgcgcgc agagtgccca cgaaacgcgg aaagctcacc gcgatacagt
     5161 ccgcgtcatc gacccagctt tcgccgtccg cgaccagccc caggacggcc gccagcatcg
     5221 caatccggtg atcccctcgg gaatcgacgc gcgacgcgcg caacggccga tcgcattggc
     5281 cttcgatcaa gaggccgtct tcgcgctcgt cgacggctat cccgaacgcc gctagaacct
     5341 gcgccagggc gtgcacgcgg tcaggctgat ccgctcgtag cacgcccatg ttcgagaact
     5401 cggtcacacc gcgggcgcgc gccgcaaggg cacaagcgat gggcagctca tcgagagccc
     5461 gctcggcaag ctcccccgtt acggggctcg cccggaggac cgaacccctt gagtttacct
     5521 caccgaacgg ctcccccaga gtatcaccgc caggtgtaat ccccatcctt ccgccgaaca
     5581 tgcggataat gtctaggatc gccgaccgag tgggattcag ccccgttcgg cgagtgctga
     5641 cgttgctgtc gggcaccagc tgcgctgcca cgagcaggaa agcggccgcc gacaaatcgc
     5701 ctggcaggtc gcacacgaaa gcgcggagcg agagcggatc tcgggggggg tggaggcgca
     5761 acacagaacc gagcgtctcg atgggaaccc ccatcgctac gagcatgcgc tcggtgtgat
     5821 cgcgggctac gacgttctcg tgaacggtgg taggccccga tgcaaacaag ccggacagca
     5881 gcaacgcacc cttggcatga tcatttgcgg acgtgagcgc atactccacc ggcgccaatc
     5941 gcgtgccagg ttcgacgccc agaaggtgaa ggggcgcaaa gtgctgcgct gattcttcac
     6001 gtgactcgat ccgggctcct cgacgccaca atggctccag ggtcgcgccg agatccacac
     6061 gctcgagacg gggtgctccc accaggcgtg attcgaacgg ttgccccgcg agcaccccgg
     6121 tgagaaggcg caaagccgtg ccagaatggc cgcaatccag cggctccgtt gccgcattca
     6181 agcccgcgag acccacgccc cgaacgcgca gtgtaccgcg cccgtcatcc tccgtacgca
     6241 cgcccaaagc gtgcagcgcg ttcagcgtcg cgaggttgtc tttcccataa ccgaagccgt
     6301 gcagaataga cggagcattc gccaacgctg ccagcaccaa cgcccgatgc gtgatacttc
     6361 gatcgctcgg cacggggacg ctgccatgca atggccgcgg agaggggcga atgacgactc
     6421 gagacattcc gttcaacgct tctgagctaa gtaggcttcg gccttttcca gtagaaccgc
     6481 caccgcggca ccatcgacgc cggccacgaa acgcacttcc ttgtcttctg cgcgcgcttc
     6541 cagcgaacga acgggttggg gcagcccgat gatggataag atagccgcgt agttcgccag
     6601 gcgctcatgc atctttcgca gattctctgc gccgcgcaag gccgcctctg gctcgtcgta
     6661 ggtcagcgtc cccgccaaat tcaggcctgg ctcttggaag ttgccgacca cggccagggt
     6721 cttcacaccg ttcaggaacg gcacctgctc gcgcgctgca gcagacagcg gctgcgaagt
     6781 gaagtctgcc ccaccggaga gcggcgcctt cggccgttcc aggacgtcga ccatccaggc
     6841 cggaagcttg cgctgcgcgc ggccctccga aatgcgatcc agcgcgcgcc gcatccccgt
     6901 ttcatctccg aacagcacgg tccgcgacgt caatacgcaa aaacccaggc tgtcggtcag
     6961 gtacatgtta cgccccgcgt attcctgaag ggtgagcttc gctcccccga acgtctgcgg
     7021 attgctggtc gcggctgcct cgatcttctc cggctgaaag gtgccggtgg ctacgcccgc
     7081 cacgtccgcc ccctgcatcg aatagaatcc gacgtagacg cgctccaaat cacgacgggg
     7141 atcgaactcc gcgctggccg gcagggggct cttggcttgc gtcaaagcga agagccgagc
     7201 accgaatcga gatgcgaata gcgcccgggc atccaacgtt gccgcgccca ccgcgttcgg
     7261 cggcagcagc gccaacggat cagcgtcgat agcgtgtcca ccgagcccct tacctgcccc
     7321 tgattcgacg attacgtccc cctgcttgcc acagccggcg gagaccagac cgaaaccaat
     7381 cccccactcg agcagttgcc ggcggctcgg cctcgtcatg acagcacctc cgtcaaggcg
     7441 cggagcattc ggtcgttttc agcctcggtt ccgaccgtga tgcgcaggaa cgtgggccac
     7501 gtccggaacg gacgcacgat cacgcctttg cgcagcagcg cgtcgtagac gccagcggcg
     7561 ggcacggaaa gctcaatcca cagaaagttc gcctggctcg gggtaacttt acaacccaag
     7621 cgcgtgagct cagcgctcag ccgagcccgt tcccgcgcgt tcaagtcccg actgcgtgtc
     7681 acgtgctctc gatcatcgag ggcggcaatg gccgcggcct gcccgggcga gctgacgttg
     7741 aacggcgccc gcacccggtt caagtagtcc accaacttcg cgggaccgac ggcataaccc
     7801 acccgcaacc cggccagtcc gtagatcttg gagaacgtac gtacgaccag cagtcgttct
     7861 cgaaggccgc gcaaacggag gccgtctacg aagtcggggg cgtcggcgta ttccacgtac
     7921 gcttcgtcga gcacgaccac gatatgctgt ggcacgcgcc gtagaaaccg ctcgagttcc
     7981 cccgcgccaa cgtgggtgcc cgtagggttg tttggattgg cgacgaagat caggcgcgtt
     8041 tccggccgga tcgcgtcagc catactttcg aggtcgtggc gctggtcgcg cagcggaacc
     8101 gcggtgaacg ggatgccacc ggacatcgcc gcgatccgat aaacgacgaa gcttggctcc
     8161 gcgaagacga tgtgatcgct cggggtcgcg aacgtgcgaa ccatcaagtc caacagctcg
     8221 ttcgagccat tgccctgaat cacctcgggc atgcttaccc caaggcgcgc ggcgatcttt
     8281 tcacgcaacg tgaaagcggc cccatccgga tagcgatgaa tccgttcgag gacctcacga
     8341 acggcggcta tcgccttggg actcgggccg agcggatttt cattggaggc cagcttgacc
     8401 gcatcggaga tgcctagctc gcgggcgagc tcctcgatcg gcttaccagt ctcgtagggc
     8461 actagcgctt cgatcgacgg caacaccagg ctcgatgcct cgcgcgccgg tgcggcgccc
     8521 gtgattggtc cggagatagg ctcggcggag cggttcgtca tgggccgcgc aggctaccat
     8581 gtcttggtca ccgcgcagcg ctcgcgttta gtcccctgac tcgagcgctc ggttgctcga
     8641 acgacgtgac tcacacgacg tgctcgaatg gctcgcgctg ctcgggaacg cagactgccg
     8701 acgtgatctc cagtcagaag gtcggctcag ctcgccgacg gaaaagagcc caggagacgg
     8761 aaatatctca cgctacgttt gacggattcg aacgcggtca cgaccggccg gtccgaggcg
     8821 tgccccgtaa tctcggcata aaagtagtat tcgaaaccat ctcggcgggc agggatcgac
     8881 tgcaatttgc ggagattgat gccacgctcc gagaaatgcc gcagcacctc gtacaaggag
     8941 cccggttcgt tctcgacgct gaatagcagg caagaaatat cccgccccgt acgcgccgcg
     9001 gggcgctgcc cggcgacacc ataacgaata cgcagatcgg gtaaatctcc cacgttttcg
     9061 cgcaaccgct ccaagcccgc gtgacgtcca cagcgctcgg gaaccaccgc cgcggaactc
     9121 ttggcttcac gcgccaagtc ggccgcgtcc ttcggcgtcc gaacgtcgat gatggtggcc
     9181 cgcggcagtt cggcgtccaa gaagcgctga caagcggcgt gggcgaacgc agtcatgtag
     9241 acgcgctcca ctcccgacat ctcatcgccc gtggacatca ggtcgtagct ggagggcaga
     9301 aaccgctcgg ccaccagcac cagatcgcta tctgccagcg acaagagagt tggttgctgc
     9361 aacccttccg ccgaggactc gaaggggaac acggcgaacg cagctcgtga acgcgttact
     9421 tcagccaatg cttcctgcgc ggtggcacac tcgatggtct gggtcgtagc cccaaaggct
     9481 tccaacgtgc tctgatggca aaaaccgccc tcgggaccca ccacggcaat ccgaaccggc
     9541 tgctcgattg cgcgccccgc ggcgcgaatg gctctgaaga tcacgcgcac gctttcggcg
     9601 ggcatctcgc ccgtcgagtt cgcgagtacc ttctcgagcc actcaccttc gcttccgtcg
     9661 gcgctgggct ccgcctcgag cagaccgtgg atcttcttgg agagcgaggc acgcgcgtcg
     9721 agcgacttgc aaatctcggc gtcgacccgg gccacgtttt cgcgaagctc ttgaacctct
     9781 cgacgccgat cgctcatggt gccttctctg ttttcccctg gtttgcccag gttggccaat
     9841 ggcctattgc gttgccagcg ggtgcccgaa agcgcccatt gctgttttgc tatccctggg
     9901 cccgtctatc cagaagattc ctgtacccag gcatttccga atcaaaccgc cctaccgcgg
     9961 gatcgcatct ggcggctaaa gtgcctcgct ttcgtgccag aacgctcggt taccagagcg
    10021 ctcggtcacg agagcgctcg gtcacgggag cgctcggtct tctgcgagca tcggaagcga
    10081 gctctctctg atcaggtcgt ggcagtcgag ctactcgagg aggtgctcga gcctgaggaa
    10141 ctcccggagc tcgagctacc ggacgtgctg gaaccactag aagaactcga agtcccggag
    10201 ctactagacc cggaactact agacccggag ccgctggacc cagagctgct cgaacccccc
    10261 ggcttggcag agctgtagag gtcagcgtac cagccgccgc ctttcaagat gaaaccggct
    10321 ccaccggaaa cttggcgttt ggctgcttcg gccgcgcaac ttgggcaagt cttgaggggc
    10381 gcctcactga tagactgctc aacctcccag gcgtggccgc acgcagtgca gagatactca
    10441 tacgtcatgt tcggtcctcg atggctctcg caatgcgtcg tgtgtgacga catgtcaact
    10501 gtgctctgca acgactagct cgtcacgcac aaagctgcgc cgtgtttttc ccgcttccgc
    10561 gcctcgctcc gtctaccgct cgtgcgtttc ttccactgac tcgttccctc gaccttcccg
    10621 aaacgttctt cgtctgggtt cttgcactac ttccgttgcg gcacctgaaa gaacagacct
    10681 ccatggctcg cgggacgtga gtacccgctc gctacggtga cctcaggagt gtttctcgga
    10741 ttcgggaatg ctgccccgac ccttgggaaa cctgtgggct tcctgtggat aagtagggtt
    10801 ggggttgtgg atagagtgtg cccctaatgc cggattggac cgccggtcta tacgtttgtg
    10861 cttgtgtgca tccgttcagg cgcgtagata gggaggagca cacgcatgac gatacatgtg
    10921 gtgcagtgac gctcggtgct cctactgggc ccgatgctca acttcagctc gcgcttcagt
    10981 tgagctttgg gttttcgagg tgcgctgagg tccccgattg agaactgttc aggttcgggg
    11041 cttagcctcg aaccgaagta attgggcgag cactcgacct caatcggccg tccgtctgtt
    11101 ttagtccgct gttgatccgt tctgttccgc tggtggaccg cgccagggct gtcgagggta
    11161 gctatcgatg cacataaaga agctcgagat cgcaggcttc aagtctttcg ttgaccgtac
    11221 cgcgatccac ttcgacaacg acgtgattgg tatcgtcggg ccgaacggct gcggcaaatc
    11281 caacatcgtc gatgcgatcc gctggtgtct gggggaacag agcgccaagc atctccgtgg
    11341 ccgggccatg gaggatgtga tcttcaacgg ttcagagtcc cggggggccg cgggcatggc
    11401 cgaggtgaca ctcaccttcg acaacaccaa cccggagtac gccgccagct tgccgctcga
    11461 gtaccgagat tactcggaaa tcgcggttac acggcgcttg tttcgcgacg gcaccagcga
    11521 gtatctcgta aacaagacgc aagtccgtct gcgggatgtc acggatctct tcctgggcac
    11581 cggcgtcggc accaaagctt attcgatcgt cgaacaggga cgtatcggtc agatcgtcag
    11641 cgcgcggcca gaagatcgcc ggcttttcat cgaagaagct gcgggaatca cgaagtacaa
    11701 gcagcgtcgg aagcaggccg agcgcaagat ggacctgacg cggcagaacc tgctgcgcat
    11761 caacgacatc gtcgccgaga ttgatcgcaa tcgggctgcc ttgaagcggc aggtggccaa
    11821 ggcggagcga ttcctcgagt atcgacgcga gctcgacgac ctcgtgttgc acgaggcggc
    11881 acaccgcctg ctcgagctga tcgtagtcga gcgggtcgaa cgggatgcct acacggaagc
    11941 cgcgcaggct tccggagggt tgcgtgaccg ggtgcgtgaa gcagaggcgg cgatcgacgc
    12001 ggcccgcctg gaagcggccg aggtggaagc gctgaccgag gatgcatcca aacgcgcttt
    12061 cgaagcggac aaccaggtca gcacgctgca cgccgaaatc gagcgcgctc gcgatcgcct
    12121 gaagcacttg tcggacaagt tggaggcggg ccgcgcggag ctcggagagc tcggtggacg
    12181 gcagagcggc ttggccagcg agctcgagct gctgcgacaa aaagtcaacg agctcgcgcg
    12241 cgacgaacgc gcgcgagaag ccgatgccct ctcggagacc gaatcgctgt cggaactgca
    12301 aggcgaagaa gcccgcgcca gcgccgacgt gcttggactg cgtcgacaat ccggcgagct
    12361 ctccgcccag gccgcggcgc tcgaggccaa agtagacggg atcctgaatc gagttgctga
    12421 cgcgcgcgcg cgccgcgatc gcttggtcca ggagcaagat gaactcaacg gcgagctgaa
    12481 caccggcctg gcccgccaaa acgcgctcga gaagagcgtt caggagctgg cacaaggcaa
    12541 gcacaccacg caagtggagc gcgaagcgct ggctcgagag agtgagagcc tccggggccg
    12601 acagctcgaa tccgagcgcg ccgtcgatgt catcaagaat gagattggtc tgaagcgcaa
    12661 tcgcttgcgg gccctccaag atctgcatcg tcgtttggaa ggcgtaggcg cgggcgcgcg
    12721 ggccctgctt tcgaagggtg atccacgtgt tctgggcttg ctcgctgacc gcgtggaagc
    12781 gccgtccgag ctgacccagg cggtggctgg tttgttgggc aatcgcctgc agacaatagt
    12841 ggtgtcggat gcagcggccg gcctcgagtt gctccaggag ttggcccgag aggagcgtgg
    12901 ccgggcaagc gtaatctccg ttcggcccag ctacgtcgcc ggcctgcagc atagcgtggg
    12961 ggtggaccct agagttcgcg gccgcttggt ggatcgactc agctacgcgc cgagcgacga
    13021 gggtttggtc cgagcgctga tcggagacgc gctgctggtg gagagcgccg gggacgcggc
    13081 ggatgtcgct cgcgtctacc ctggagtcac cactgtcgcg ttggatggga ccgtagttca
    13141 tccggatggg gtaatcagcg gaggagatgg cgacgacgtt gcctccgcaa tgctcgagca
    13201 gaagcgtgaa atgcacgagc tggcggacga cctctcgcgc ctcgaagctc aaggtgagcg
    13261 cttggtcgcg gaccataacg ccattcgggc gcgtgccacc gaggtgtcta cggcgctcga
    13321 tcgtgcacga caggaagctc acgaaggcga gctcgcccac gtcaccgcag aaaaggatct
    13381 cgcgcaaacg gccgcggaaa ttacccgctt taccgcgcgc ttgggctcga tcgcgacaga
    13441 gctcgccgaa atcgaccaca tgctggcggc tgcggaagca tccgaaggcg actcgcgtag
    13501 ccagctcgat gggatgcgcg cggagctcga gcacgttggt catgcgctgg tgaaggcgga
    13561 ggacaatgcg agcgtgtggc gcgagcgtgt ggcagcgcag gcctcgctcg tcaccgagcg
    13621 ccgcgtacgc ctcgcgcagg tgaaggagca actcgatgct gcgcgccagg cgcacgagcg
    13681 cgtagagaac tccttgctgg agctcgagtc gcgcgggcag cgcttgcaca ccgagctcga
    13741 cgaagcagcc gcggtatacg gtgagacggc aggtagaatc gtgctcgccc gcgcggccag
    13801 ggccgacgcg gaggctcaag cgcgcgaggc ccacgccacg ttcgaggccg cgcgtgagcg
    13861 actcgagcaa tctcgctcca cgctcagcaa tcaagagacc gacacccgac gtcaaaggga
    13921 cgaactcacg acgctggagg agagcgctcg aaagcacgaa atggcgatgc agcgcttcga
    13981 gctcgagcgc gagcatttgc tgactggcat tcgtgagcgt ttccgcggcc tggatttggc
    14041 gcgcgtggtg ggtgattacc acgcccggcc cccgacggat gccgaacaca agcgcaggat
    14101 cgatgagctg actcagctca tcgatcggat gggtccggtc aacttggatg ccaaggccga
    14161 acaccaggac gcggagcgtc gtttcaaaga gttgaacgac caaaaggtcg acatcgagaa
    14221 ggcgctcgag gagctcgatc aagcgatccg gcacatgaat cgcgaatcgc gcaagcgctt
    14281 ccgcgagacc ttcgaagagg tcaacacgct gttcaagaag accttctcga agttgttccg
    14341 cggcgggcgc ggcgagttgc agctgacgaa tccggaagac ctgctgaaca gcggagtcga
    14401 gattatcgcg cagccacctg gcaagaaaat cggttcgatc gaactgatga gcggcggtga
    14461 gaaagcgctc acggccgtgt ccttgatctt cgcaatcttc cagcatcgcc cctcgccttt
    14521 ctgcattctc gacgaggtcg atgcaccgct cgacgaggcg aacgtcaacc gttacaacga
    14581 gatggtgcgc gccatgacgt cgcactctca gttcatcctc atcacgcaca tcaagagcac
    14641 gatgcaaagc gtggacgtgc tgtacggtgt cacgatggcg gagcctggcg tctcgcgcat
    14701 cgtgagcgtc aaggtcaacg agcacgccgc ggctcgtagc ggttcgagca tgactcgtgc
    14761 gcctgcccga gaaggcaccg gaagcagcga gggtacggtc gcgggcgtcg cgtaaagcga
    14821 gcgaacgcgt gatcgtccag cggatcacca tcgaagccat cggttcggcg gtcaggacgg
    14881 gtgccattag tggaagtctc gcgacatttt gccgatgtca cgacccggca cgtcgagacg
    14941 ctccaagcgt tggacaatca agtgaatgac cgaccccgta ttggggacga cttgtcgttc
    15001 gagtttgcct tccgccatta ggatagcgga ctgagtagcc acccaacgtt gttgttcgaa
    15061 cacgtttcgc cacagcacca agttgatgaa acctgtttcg tcttcgagcg tcatgaatac
    15121 gacgccactg gcggtagccg gttgttggcg acaaattacg actcccgcca ctttcacggg
    15181 tttgctctgc gaagtgtcca ccagttcgga cgcacacaag atgccgtctg ccgccaagcg
    15241 gggccgtaag tggcggatcg gatgatcgtt cgccgagagg cgctttctcc gataatccaa
    15301 caccaacgtc tctgcggcgc gcagctcggg caaccggggc ggcgtctctt tcaagctctg
    15361 cttttcgaat agcccgctca agcgtggcgc gcgtccgagc caagcggctt gcctccgtcc
    15421 aattacgagt gactcgaagg cgcccgcttc tgccaatgca tcgatctccg cacggtccag
    15481 ctcggcacga cgtgcgacgt cctggatgtc actgaagcgc cactcggaac gcgctgcttc
    15541 gatgcggcgc acggctttct cgcccaaccc ctttacgaga cgaaagccga gccgcaacgc
    15601 ccgctgagct cccgtcgcgt gcggcccgat ccccgagcaa gctcccgctg gctcgagctg
    15661 cgtatcccaa gtgctttgcg cgatgtcgac gtcacgcacc tccactccat ggcgccgtgc
    15721 atcccccaaa atcgtggcgg gtgagtaaaa ccccatcggt tgtgagttca agagtgcaca
    15781 cgcaaaatga gccgggtagt gcgccttctg ccaagccgac gcatatacca agagcgcgaa
    15841 agacgaggcg tgggactcgg ggaagccata gtcgccgaag cctttgatct gctcgaacaa
    15901 agcttcgcca aactctcgtg agatgccatg ttgctcgaag ccttcgagca aacgcgagcg
    15961 gtgccgcatg agtttgccgt tgcgtcgcca ggccgccatg tctcgtcgca gttgatccgc
    16021 ctcccctccc gaatagtccg caccgacgat cgctatttgc atgact
//
LOCUS       DASTKU010000782         3306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1270840, whole genome shotgun sequence.
ACCESSION   DASTKU010000782 DASTKU010000000
VERSION     DASTKU010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270840"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1742)
                     /gene="dnaB"
                     /locus_tag="VFQ61_27340"
     CDS             complement(210..1742)
                     /gene="dnaB"
                     /locus_tag="VFQ61_27340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET9958251.1"
                     /translation="MSYAAAESKGPHKGPPKPSAADLIPAPGRVPPNDLDAEAAVLSA
                     ILLDGSAFDQVQDLLRPEHFYADANRRVYEAVLDLQGAGRPVDIVSVAGYLRDRGRLE
                     QVGGTPYLAQLSDATPAVAHIGAHAQVVREKWRLRQLIATCQRFAAEGYAAPPDVQKF
                     IDSAEQSIFDIARTPEGTTVEPVRDAVVRAFKILEEAERRGGGVTGISTGFSRLDRQC
                     AGLHRGDLYIVAGRPGMGKTSFVLNVALNVASAKVQAGVTAEEEYFEGGQEQLGHGVL
                     FCSLEMPKEQLAARLLASEAYVDMSNIRSGRMSREDWSKMTEAASRLARLPLWLDDTP
                     GLTLLDLRAKIRRLQAEISRGEGSVRAKSLGLVAVDYLQLMQGRKDAGSREQEISELS
                     RGLKGLAKEMRVPVMALSQLNRSVETRTAKDKRPQLSDLRESGAIEQDADTIMFIYRD
                     EYYFKESEAKGVAEIIVAKQRNGPTGTVLTKFTSAFTRFDNLAAEEFDVDQFDDFAGD
                     GA"
     gene            complement(1919..2374)
                     /gene="rplI"
                     /locus_tag="VFQ61_27345"
     CDS             complement(1919..2374)
                     /gene="rplI"
                     /locus_tag="VFQ61_27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET9958252.1"
                     /translation="MATNVKVLLQADVDNLGTGGEVVRVRSGFARNYLLPRGLALPAT
                     AGNLTRVEELKRAAAQRAQQEREKAAEVASKLNGLTLKLSRAVGEEGKMYGSVTAKDI
                     ADAAARAGTEIDRKRIALADPIKQLGTFEVPVKLHPEVTATLKVEVSKS"
     gene            complement(2385..2681)
                     /gene="rpsR"
                     /locus_tag="VFQ61_27350"
     CDS             complement(2385..2681)
                     /gene="rpsR"
                     /locus_tag="VFQ61_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET9958253.1"
                     /translation="MKEQQRERASERPDARGGGDKEGGTAFRRQRRRGCNFCSDETLA
                     IDYKDAQSLRYFVTERGKIVPRRISGTCAKHQRSVTLAVKRARNIALLPFTSND"
     gene            complement(2678..3217)
                     /gene="rpsF"
                     /locus_tag="VFQ61_27355"
     CDS             complement(2678..3217)
                     /gene="rpsF"
                     /locus_tag="VFQ61_27355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET9958254.1"
                     /translation="MTATQATPMKAREYETIYVLRPDVPRESQERIAQRLTEVVSRES
                     GKLTTIENWGRRQLAYTVSKYRRGVYVYLKYLGGGALVAEVERNLRMLDDVLKYQTVQ
                     IARDIDVAAVDVRPENVQFEAVEPATQEELAEPIERLLGLEQAPERERSNDGGEDLDG
                     EEFEGEDLVANPDVAEDRS"
BASE COUNT          552 a         1114 c          995 g          645 t
ORIGIN      
        1 ggatctgcca gacgtgcgcg aggtgcacgc tcgcgtgcac cccgagtttc agaagctcgg
       61 catcgactta cacttggtga gcgcggtcac gcacgagggc ttggacgcgc taatgcaggt
      121 gttggccgag aagctcggaa tctaagaatc cgagcttacg gcgcctcccc cgcgagcgcg
      181 cccgagcgct ccgagccccc gccaacagct caggcgccat cgcccgcgaa atcatcgaac
      241 tgatcgacgt cgaactcttc ggccgccaag ttgtcgaagc gcgtgaacgc cgaggtgaac
      301 ttggtgagca cggtgccagt cggaccgttg cgttgtttgg cgacgatgat ctcggccacg
      361 cctttggcct cgctctcctt gaagtaatac tcgtcgcggt aaatgaacat gatggtgtcg
      421 gcgtcctgct cgatagctcc gctttcgcgc aagtcgctga gctgagggcg cttgtctttc
      481 gcggtgcgcg tctccaccga gcggttgagc tggctcagcg ccatgacggg cacgcgcatt
      541 tccttggcaa gccccttgag gcctcgagag agctcgctga tttcctgctc gcggctgccc
      601 gcgtccttac ggccttgcat cagctgcaag tagtcgaccg caaccagccc caagctcttc
      661 gcgcgcacgc tgccctcacc gcgcgagatc tccgcctgca agcggcggat cttggcgcgc
      721 aaatccagca gcgtcagtcc tggcgtatca tcgagccaga gcggtagacg cgcgaggcgc
      781 gaggctgctt ccgtcatctt gctccagtcc tcacgcgaca tccgtccgct tcggatgttc
      841 gacatgtcga cgtatgcttc ggaggccaag agcctagccg cgagctgctc cttcggcatt
      901 tccagagagc agaacagcac tccatgccct agctgttctt gcccgccttc gaagtactcc
      961 tcttccgcgg tcactccggc ttgcaccttg gctgatgcaa cgttcaacgc cacgttcaaa
     1021 acgaacgagg tcttgcccat gccggggcga cccgcgacga tgtacaagtc gccgcggtgc
     1081 aagccggcgc actgtcgatc caagcgtgag aagccagtcg aaatgccggt tacgccaccg
     1141 ccgcgccgct ccgcctcctc taggatcttg aaagcgcgta cgaccgcgtc gcgcacgggt
     1201 tcgacggtcg tgccctccgg cgtacgagcg atatcgaaga tcgattgctc cgccgaatcg
     1261 atgaacttct gcacgtccgg cggggccgcg tagccctccg cggcgaagcg ttggcaggtc
     1321 gcgatcagct gccgcaatcg ccacttctcc cgcaccacct gcgcgtgagc cccgatatgg
     1381 gctaccgcgg gcgtggcatc cgatagctga gccaaatacg gcgtgccacc gacttgttcg
     1441 aggcgtcccc ggtctcgcaa gtagcccgca accgaaacga tgtcgacggg ccgccccgcg
     1501 ccctgcaggt ccagcacggc ttcgtacacg cgccgattgg catccgcgta gaagtgctct
     1561 gggcgcagca gatcctggac ttgatcgaac gcgctcccat cgagcaggat cgcgctgagc
     1621 accgcggctt ccgcgtcgag gtcgttcgga ggaactcgtc ccggtgccgg gatgagatcg
     1681 gccgctgatg gctttggcgg ccccttgtga ggacccttac tttcggctgc tgcgtagctc
     1741 aaacctttgc ctcccgcgaa cccgaccgga ttctcccaaa cgtccagagg ttatcaacag
     1801 ggcactcaaa tggcgcgcgc cccggagcca aattggcgac cggggcgcga cttacattcg
     1861 agactgaccc attcgagact gacctggcgg accgacctca cgggccggcg ctaggcgctc
     1921 agctcttgct gacttcgacc ttgagggtag ccgtcacttc agggtgcagc ttgacgggaa
     1981 cttcgaaggt tccgagctgc ttgatcgggt ccgccagcgc gatgcgcttg cgatcgattt
     2041 ccgttccggc acgggccgcg gcatccgcga tgtccttggc cgtaaccgat ccgtacatct
     2101 tgccttcctc accgacggcg cgcgacagct tgagggtcag gccgttcagc ttggaagcca
     2161 cctcggcggc tttctcacgc tcttgttgcg cccgctgtgc tgcggcgcgc ttgagctctt
     2221 ccacgcgggt gagattgccc gcggtggccg gcagcgcgag accgcgcggc aacaggtaat
     2281 tgcgggcaaa accggagcgc acgcgcacga cttcgccgcc ggtacccagg ttgtcgacgt
     2341 ctgcttggag cagcactttg acgttggtag ccatgcgaaa ttcctcagtc gttcgaagtg
     2401 aagggcagca acgcgatgtt gcgcgcgcgc ttgaccgcga gcgtcacgct gcgttggtgc
     2461 ttggcacagg tgccggaaat acgacgcggc acgatcttcc cgcgttcggt gacgaagtag
     2521 cgcaggctct gcgcgtcttt gtagtcgatg gcgagggtct catcggagca gaagttgcag
     2581 ccacgccgac gctggcgccg gaatgccgtg ccgccttcct tgtccccgcc gccgcgagcg
     2641 tcgggacgct cactcgcacg ttctctctgc tgctctttca tgaccgatcc tccgccacat
     2701 ccggattcgc tacgaggtct tcaccctcga actcttcacc atcgagatct tctccgccgt
     2761 cgttcgagcg ctcacgctcc ggggcctgct cgagtccgag caaacgctcg atcggctccg
     2821 ccaactcctc ctgagtcgcg ggctcaacgg cctcgaattg cacgttctcg ggccgcacgt
     2881 ctaccgcggc gacgtcgatg tcgcgcgcga tctgcaccgt ctggtacttg agtacgtcgt
     2941 cgagcatacg caggttgcgc tcgacctcgg cgaccagggc cccgccgccc aggtacttca
     3001 agtacacgta cacgccgcgg cggtacttcg acacggtgta cgccaactgc cggcgacccc
     3061 agttctcgat cgtggtcagc ttgccgctct cgcgggaaac gacctcggtc aaacgttgcg
     3121 caatgcgttc ctggctctcg cgcggaacat cggggcgcag cacatagatg gtctcgtatt
     3181 cgcgcgcctt catgggcgtc gcttgtgttg ctgtcatcac atctcctcga ggacaactgg
     3241 cccccgccaa tcgaacagcg ggaacaagga gttcaacgcg ctttcccggt ttgactcttg
     3301 cccggg
//
LOCUS       DASTKU010000783        24744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_154276, whole genome shotgun sequence.
ACCESSION   DASTKU010000783 DASTKU010000000
VERSION     DASTKU010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 24744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24744
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154276"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VFQ61_27360"
     CDS             complement(<1..520)
                     /locus_tag="VFQ61_27360"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9958255.1"
                     /translation="MRPSKSRELGAPPNQEQPRELPYELVTYEGRQPHRVGLPHHIRL
                     PSCGSVLIGRAPDCEVRIENVSVSRRHAIIHLNQLEVEDIASRNGSALFSDAPSWESV
                     AEAAPGAGRVLEPGRRYPLRPGDFLKVGSVVLTLKRATQPQHTEPVLHEEPLNESEVV
                     VQGAVMRDLYEVA"
     gene            866..2161
                     /locus_tag="VFQ61_27365"
     CDS             866..2161
                     /locus_tag="VFQ61_27365"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9958256.1"
                     /translation="MRNSHKFAARMLVSIVLPCATFACSGSDDTAVDKQPSGQIEIFS
                     WWVSGGEVDALNAVLDLNQEKYPKTKVKNLTEDLADEARQRLEQRMAQGAPPDTFQAN
                     VGADLFKWVLFNGVDDKESKVEPLNAIAEANGWFDVFPKSVTDALSHNGSFYGVPANI
                     HRLNSLFYNTKLFEREGLTPPDTRDELLTVSKALKAKGYVPLCIGSDHWWTLSLLAFE
                     NIFPSVAGGDYYLGYWTGKKTPDSAQVNAALDYLEELWPYFNEDANSLDWTTGIDHMF
                     DSTNPCAMTVMGDWAKGYLEKKGWEAGTDFAQIPFPGSRGTFVFTADTFPLPKGAPNR
                     PGAVALLETFASVEGQIAFNRIKGSIPVRTDIDPKEFDSIAQKTMADFTKDQLVSAMS
                     GLLPQDSFGDLGPALKEMLSSGDREGVLNALANDYAMLK"
     gene            2266..3981
                     /locus_tag="VFQ61_27370"
     CDS             2266..3981
                     /locus_tag="VFQ61_27370"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9958257.1"
                     /translation="MAMPQLTHTPAGPTELGGKYRLLLDLGQGGTANVCLAVARGPSG
                     FSKLVVLKMMRSNLADEGEFSKMFLNEARLSARLNHANIVQTNEVFEHEGRPVLVLEY
                     LEGQSLSAVMKRARDGTRFSLNMQLRVLSDMMSGLHYAHELADFKGTPLGIVHRDISP
                     QNVFITYDGQVKLLDFGIAKLSESHVETATGVLKGKVRYMSPEQVVGQKVDRRTDIFA
                     VGVMLWEAATSTRMWAGLGDTTIIHRLLNEPLPRPSSVNPAVDPELERIIVKATAAER
                     EQRYDTVADLQRDVDELLSKQNVSVRQRDIGAVVTALFEDTRQRSRRLIEAQLDKVAT
                     LTDAEYAAVQPPELSSLGVTRTGRTELEPSTTGASSKVVDTQPRAFGVNASGKRKALV
                     LGAIACLGALVAVGFASGVGSKPVQAVAAPPVSPAAVSNSVRLRITGFPAAAKLYLDE
                     QLLPSNPYSQDVPRDTKKHVLRVVAEGHREEQRELTLAEDSEVVVALGALPQSSTPSS
                     GEKVASNSPAKKAAPMEPIDPRRVRSTPIAEAPAKPKPEANSDCSPPYIVDSKGVKRY
                     KPNCF"
     gene            complement(3992..4765)
                     /locus_tag="VFQ61_27375"
     CDS             complement(3992..4765)
                     /locus_tag="VFQ61_27375"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HET9958258.1"
                     /translation="MPSLTNSHATDERLGAKSTAGNGSLTPVEPYALLTGALEFSLAS
                     LILEYPTREVVLTLVELQESDSCAPALLALASACRAGLRHVQVAYAALFEGVQSKVSL
                     YETEHGVMRGAAKGKDLADIAGFYRAFGLTPNEENAHEMLDHIAVELEFYSQLLIRQS
                     ALATARDAEGCEIVEDARRKFLHGHLGRFAGVIAKQPAVQECPVYGPALAWCAALVQL
                     ECERLRVEPPPLDFFPLRETSEEEAMCGAVHLPILQSAP"
     gene            complement(4803..5687)
                     /locus_tag="VFQ61_27380"
     CDS             complement(4803..5687)
                     /locus_tag="VFQ61_27380"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HET9958259.1"
                     /translation="MSKTITIAIFLGSMTAAVGCRNSAPPSASDALPAPELQQLNVTR
                     VKADLSQPDPDAPFWADVPRGSITLTAQPLVNPRPESTTTEKVVIQAVHDGKRAAFRV
                     VWSDSEPSAAGKLAEFSDALALQFPANGSASTPVMMGGPGLPVHILHWRAQYQADQEK
                     GKPEISGVYPNANVDMYAMDFKDAPSGSAAEKESFSPARVVGNPQSYPKQAVDEVVAE
                     GFGTSAVQQNGHAQARAAWKAGRWTLVITRTLEVPGRSSLKSPGESQLAVAVWQGGKG
                     EVGSRKSLTMSWLPLKLR"
     gene            complement(5696..6832)
                     /locus_tag="VFQ61_27385"
     CDS             complement(5696..6832)
                     /locus_tag="VFQ61_27385"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET9958260.1"
                     /translation="MAKVNNWQLRRDVDYVYEGKRPKKQWAIVFDLNKCIACQTCTLA
                     CKTTWTSGKGQEYMFWNNVETKPWGGYPLGWDIRVLEKLGRQDWGQNGQYLGKTLFEA
                     APPTERVLHFSPTDEDWMYPNVGEDDCAGGTLDTGAHLAGETHDKWFFYLPRTCAHCT
                     YPACLAACPRKAIYKREEDGIVLIDQARCKGYGECVRACPYKKSMYNPYTRTSEKCIG
                     CFPAVEQGYQPQCVVNCIGKIRVMGFISPPAVARKDNPVDYLVHEKGLALPYYPQLGL
                     EPNIYYIPPIHADPEYLVQMFGPRVHEAIARYRDLSRDARAQGLLCLIGSTDRIIHRF
                     EVKAGRASGYDELGQLIVEVPVTEPVHQRAPFDAELGAIRNNTP"
     gene            complement(6838..10311)
                     /locus_tag="VFQ61_27390"
     CDS             complement(6838..10311)
                     /locus_tag="VFQ61_27390"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9958261.1"
                     /translation="MSDPSFPRRSFIRALAGGAALATAHGLAAPLRFLKPLSFANPLT
                     SYPSRDWESTYRDIFKADSSFVFLCAPNDTHNCLLRAHVKNGIIVRIGPTYGYGKAED
                     LYGNKASHRWDPRLCQKGLALVRRIYGDRRVKAPMLREGFKAWADAGFPRDPSTGKPD
                     PKYFQRGKDNWVRATWEEAYDYSARALDNIARAYSGETGKQRLNVQGYDPAMIEALDG
                     AGTQTIKVRGGMALLGVTRIFGLYRFANLLALADGKIRGVGADRAHGARGWDSYSWHT
                     DLPPGHPMVTGAQTNDFDLFTVEHSKLCIVWGMNWITTKMPDSHWLTEARLKGTRVVA
                     VTVEYSATASKSDEVVVIRPGTDTAFALGMAQVIISKKLYDPAWVKTNTDLPFLIRMD
                     TLEPLRPEDVAPSALPGYVSHRPTNLALYPQDQKPAPALEHKAQLASEGMSQDFAAYL
                     ILDERTKRLTAVTRDQVGAKARNTSPALSASLEVELRSGEKVQVRTHFSLLSQYLDEN
                     MTPDQVARITGTPVSAIESLARQVAQNPERTTIAVGMGANQFFHSDLKDRAILLVAAL
                     TRNLGFPGGNVGSYSGNYRTSIFGGMPYYVAEDPFNLQLAAGGEVTKRKYSGYESLHY
                     FNYGDRPLRQGNKLFTGKGHVPTPTKAMWLSNSNSVIGNAKWHFDVVNNTLPRIEFIA
                     FSDWWWTGSCEYADVVFAVDSWAEFRYPDMTASTTNPFVQTFPTSKIQRVFDTRADVE
                     VLAGVSKALGKLYSDTRFAEAFKFIDENNAEEYLQRIIDHSFALKGYSVKNLHQLAQQ
                     GIPALLNVRTYPRASSYEQQRGEQPWHTKSGRLEFYRFEPEFRESGENLVVYREPIDS
                     TFHEPNVIVSGPHPAVHPTGPEAYGLNRDDLSTEVRQVRHVQRSVEQTLASRHPLMDK
                     GYNYIYHTPKYRHGAHTTPVDTDFISVLFGPFGDIYRHDKRMPSVTEGYVDMHPLDAK
                     ELGVEDGDYVYVDADPIDRPYRGFKPGTEAYKVARLLLRVRYYPGTPRRVTRTWHNMY
                     GATFGSVKGHEKRVDGLARNPVTNYQAMYRFGSHQSATRAWLKPTLMTESLVHKDMYG
                     QELTKGFLADVHCPVGAPRESFVRMTRAEGGGRGGQALWRPAALGLRPTYETDAMKLY
                     LRGRFTSVQGK"
     gene            complement(10354..11112)
                     /locus_tag="VFQ61_27395"
     CDS             complement(10354..11112)
                     /locus_tag="VFQ61_27395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958262.1"
                     /translation="MNTAISDLARRWCGARRAALRAFVPDASAEASETELLPAALDID
                     AEGVLRRVFDNSERLGGSFRQYYTADLTVLDLTRILPALQVPCFAFAWTDQSDPPHYR
                     GQRKGCASRALHGRTCDYFREAGQGLVLGLTSSVHHARHESLNCGDAECVDVLFVHAQ
                     SPARFGPIPAPIRLRLATLEQTVKMFDSSARLRWLGISERILYYTLEHAGCDNVDLNA
                     VVARSIKRRCPQLGACNASPKSVLSSAPPQACAR"
     gene            11407..12138
                     /locus_tag="VFQ61_27400"
     CDS             11407..12138
                     /locus_tag="VFQ61_27400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET9958263.1"
                     /translation="MTSATIKQLLARTPVLEKCTEDVLETLAAHASSREHSRGDVLWQ
                     VGAEPTALVVVSKGLIKMTRVGGVGRRSLCGCFGPPQSIGDGVLVRGSPYPAQAAVAT
                     PRAATIWVPRGLFLEAMQRCPELGVSMCRHLQGKLMALHDKIEVLSSGDVEARLATAL
                     LKLADQYGDDFIDGSHLVPVALSRRELADWVSTSVETAVRVMTRWERLGVVTTTRQGF
                     LIRSLEELKRARGDVAGESISPLAE"
     gene            12165..12602
                     /locus_tag="VFQ61_27405"
     CDS             12165..12602
                     /locus_tag="VFQ61_27405"
                     /inference="COORDINATES: protein motif:HMM:NF021192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase associated protein"
                     /protein_id="HET9958264.1"
                     /translation="MSWDGIYIFESESERNCWLPMAARRALDMAGIHVSLEPWKGLSA
                     EQRQGFINLGRAPTVDVAAVGRRVEELGLASRPEQKWTEPGADEVPRDVQLALAPQLT
                     ISKAEWSQLSPLDRYTLYKLASRERTDKAKLAWREIRARSFGK"
     gene            complement(12745..13170)
                     /locus_tag="VFQ61_27410"
     CDS             complement(12745..13170)
                     /locus_tag="VFQ61_27410"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET9958265.1"
                     /translation="MRKTSFVIMMACALLPSCSKNSSESSGSYAVPEPAAQGALAKGA
                     EAPAQSPQTVFNTKCTVCHGSVGAGDGPGAAALDPKPRAFADPAWQNSVTDEQIKKAI
                     LEGGAAIGKSPGMPANPDLSAKPDVVNGLVAIVRGFKKG"
     gene            complement(13507..14526)
                     /locus_tag="VFQ61_27415"
     CDS             complement(13507..14526)
                     /locus_tag="VFQ61_27415"
                     /inference="COORDINATES: protein motif:HMM:NF020968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HET9958266.1"
                     /translation="MAEEPKRVTVAELRPPRPAVTYPAVMGTLEHLPFPKISSGGTAS
                     ASRDDWVASEKIHGAQLVVGANADEVRTGKRKAWLAPDEPFFGWQMLRPLLNDAARAI
                     QRNLDPTGVVWLYGELFGGSYPEPSVTPVAGLVPVQTGIWYAPDLHYAVFDMVHQASG
                     AEPVFLSDAQTRALAEAAGITTVPLIAKGRYSDLDRLPVRFPTLIPALWGLPSIPNNY
                     AEGYVLKYAQQTSVDERPVTKRKIPEFCEQRFDESNAFDANTHLEFEALSALAKNLMN
                     AARVASARSKVGEHPERVNEEAVLDALIDLRDMLPRQFDTMSAEDEQRLQHQLDSRAR
                     EVVAG"
     gene            complement(14651..18070)
                     /locus_tag="VFQ61_27420"
     CDS             complement(14651..18070)
                     /locus_tag="VFQ61_27420"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HET9958267.1"
                     /translation="MTHADSGLRQVNTVARRSLRTVSFCLLPLAIAACSTAQGGDSDL
                     GAASQAVEGADCGFSVTSDVHGINKKQFHAKIRVKNSSGATSTNFSVLIDAGGAQLVQ
                     SSKGTFTATENGYLLKPGEKTLGELEDGDDYQFELKFEGTYTRLNAYIQSNNGVACDQ
                     AAPTLKLTASGDFFTSAGSLRLSATATDNVAVSKVVFAQDGVPIGTATTAPYTLNVPI
                     SKALNGRHRYSATAYDLSGNQGGETKRVLVAIDNKFFGSISTTAADYTNFTAHFNQLT
                     PGNAGKWGSVEATRDQMNWTDLDAAYSFAKANKIPFKLHTLVWGQQQPNWLDGLSPEE
                     QLAELEQWMSALAERYPDVQMIDVVNEPLHAPPSYAAALGGAGATGWDWVIKAFEMAR
                     AHFPNAELILNDYSILTMASTTQDYLKVVKVLSDRGLIDGIGEQAHFYERAPELSVLT
                     ANLNAFAATGLPIYITELDLNFADDARQAIRMKELFTAFWSNRSVLGVTHWGHRQGNM
                     WQENAYLIRTDGSLRPALTWVECFKAGGTNCPVPAYVPQPRTGDDSGITLEAEQYDSA
                     HALLPAGEVVAYASNGSWFAFDQVAFRNNWDSLNVTYAQGGTSPVQLTVHFDSLDSPP
                     VATVPLAPTGSWGTSKTVSVPWAPTNVQKNVFVRFNGGGANVDKLQFAAPSGTGANLL
                     TDSDLELGTKAGWSSWGGGTIANTTARALTGTHSIAMTARNGNSPLVQTLTSAVAPGR
                     TYKVSLWATVGGAASAAYVTTALQCVGGETTYGRLGGWENTKTLSDGQWQEFAGDLVV
                     PDCQLANVAFWLEGPAAGVDLYIDHASVRGVTTRNVITNGTFESGTSGWFTWGGATLT
                     AASDRVHGGAKSLLVSGRSGNYPAATDLTSVVKAGSSYPFSLWVSVRSPDGTAKAINV
                     TQATTCKAADGTTSTSYAWIAGPTNVASSADWVQLAGTIAVPNCTLTQLQLFVEGGAG
                     ADLYVDDVQVMDNSAASGNLITDGTFESGPGGWGGWGYTSLAVVNTTSHAGSSSLKGT
                     GMQPNGALSRDIKPVVSAGKRYRATAWVSVGNLAAGSGSVKFQTIQSCNATGSDSYPW
                     LSGDTVTNGSWKQLTGTVDLSACSSVEKLQLFVGADGGDLYVDDVTLTPIP"
     gene            complement(18634..19995)
                     /locus_tag="VFQ61_27425"
     CDS             complement(18634..19995)
                     /locus_tag="VFQ61_27425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958268.1"
                     /translation="MMKSSHRAWTWSVERAKVVAWAVLFGTAGCSAAKEDAADADPLT
                     DALSRSEQELTISRLPNNFPLPDASGTAATFSTAGFVDLENEFHVPQGQNGRSCESCH
                     LPQAGWSVRPIDVELKFALTQGNDPIFNLLDADSPNADVSTPRARYTAYSMLRKGLFR
                     RGGAVPANAEYEIIAVDDPLGAGGSLTRFEAFRRPLATANFHIAKNIGWHDQNTNGSG
                     DIPAGLTSQATGNITGAQQGAAAPPETIASIVNYELGLSFAQQYVFGVGFLDSCGARG
                     GPQNLSQQPAVAGRFDLFDAWAELAPGSCTTRAADRRRQQIARGQKLFNEANPGGGSC
                     SGCHNAANNGSNASGRLFDIGASRSAFRKPGMPLYTLRNKTTLEERQTTDPGRALRSG
                     RWADLDAFKTPSLRGISARPPYFHNGIAPNLLEVVRHYETALGFVFTAQEERDLVAFL
                     EAL"
     gene            complement(20183..21064)
                     /locus_tag="VFQ61_27430"
     CDS             complement(20183..21064)
                     /locus_tag="VFQ61_27430"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9958269.1"
                     /translation="MLSAHSSAAQELTDRLAQSLKLEADTAMNESRFKAALDAYERAA
                     AIESSPILDYNRARALQGLGRHADALMYLESFSDRATPELKGRVPQLSALFATLQQRV
                     GKLRISCNVAGAHLRVGERSLVMSAPSESLRLDDGFYQVTASAPGHSEITRTIQIPAQ
                     GVATLALELPRVDDAAFVTIASNVPGARVTVDGNTVGQTPLEMRLAAGSHLVQLRSDG
                     YEPRDSQIVLAAREKRHLQWPLQRNTPVYARWWFWTGVGVALAAGTLTAVALKTDRAA
                     DRGDIPPGTIAAPLTGF"
     gene            complement(21123..22631)
                     /locus_tag="VFQ61_27435"
     CDS             complement(21123..22631)
                     /locus_tag="VFQ61_27435"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9958270.1"
                     /translation="MFDSGALIDGKYRIVKRLASGGMGVVYLAHHERLDRQVALKILL
                     PERATRELGERFRREVRALARIHSPYVAQAYDADLLPTGEWFLVMEYLQGRDLRNEIS
                     SRGALSTEEAVTFVCQASRGAAAAHKVGIVHRDLKPQNLFVTELENSLRIKLVDFGIA
                     RELNAADPALTEPDLSLGTPLYMSPEQILTPFAIDARTDVWALGVILYELLAGFPAFS
                     AATPGGILLAITSQEPRPLREVNAQIPPELVNVIERCLQKDPNARPATACELAELLAP
                     FTISEERLKQLAAAVARREEPLSRVPLDNAQASALRSQQGGSRSAPVSPSPPTLDVVN
                     NHVPDATTLTIVATVQPALEGTKERAISKVLCATHRGPLRRRLVAAVTTLAFILVSIS
                     LVEAWRRSRVGTSQNASSAPLSSTIQPHSSAAPVATPKNDSTGSTRPDRGPETQPTSS
                     PPLSASSVPTTPASAPAVRFPRNGVRRSPERTLPTRPAPSAPSNNDGNPLHL"
     gene            22833..24560
                     /locus_tag="VFQ61_27440"
     CDS             22833..24560
                     /locus_tag="VFQ61_27440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9958271.1"
                     /translation="MGCEQPTEVFVEISTDADCRDVRDTSLASGRLIEIEGKVPTTVT
                     QRCDEQTGRIGSIVVVPSGDENQEFALSVVTGFTKSAQECVRDGYLGGCIVARRATRF
                     LPHERLDLPILMQASCIDVPCSASQTCRDGRCVSATLPDPRSCVDPAGCPPSGARGGS
                     TTGSGGMSQASGGLGGASSLAGGSSSGGASSSGGAFSVAGTQTSGEGGAGASSSDGGE
                     PSSDAGGAPEGGGNQGGNESGGQPGLGGSLANGGSFGGTLVSLEGGTAGSTGSAAGGT
                     TVGGTTLGGSQNAGTSGSSSGASSGGASTGLGGNTSIGGTQSSGGTQSSGGTQSSGGT
                     QSSGGTQSSGGTQSSGGTQSSGGTQSSGGGVPMSVCGNGLVDVGETCDDGNSIRADGC
                     AQCSAQIGWACTGVPTRCSVLHTASFQVSGNDNIPADGLRNWFAALPYDSSWYLSVEI
                     DTSLTKGTQFCLSRGDWYADAYLKFAGTQAGGSMSSGIWSKWWRTKDGLWSLPDVLPH
                     LNSFGAACDLDDLQAFCVESLLGLQEIAVLPGNAGWTEAYNGGFAPLGSAWQVRLALA
                     PSRQEACGF"
BASE COUNT         4565 a         7565 c         7893 g         4721 t
ORIGIN      
        1 cggcgacttc gtacaagtcc cgcatcaccg cgccttgcac cacgacttcg ctctcgttga
       61 gcggctcttc atgcagaact ggctcggtgt gctgcggctg ggtggcgcgc ttgagggtca
      121 gcacgacgct gccgaccttc agaaaatctc ctggtcggag cgggtagcga cgacccggct
      181 cgagcacgcg accagcgccg ggtgcggcct ccgcaacgct ctcccagctg ggcgcatccg
      241 agaacagagc gctcccattt cggctcgcga tgtcctcgac ctccagctga ttcaggtgga
      301 tgatcgcgtg ccgtcgagat accgagacgt tctcgattcg gacttcgcaa tcgggagccc
      361 gcccgatcaa aacactgcca cacgacggga ggcgaatatg gtgcgggagg ccaacacgat
      421 gcggctgccg accctcgtac gttacgagct catagggcaa ttcgcgcggc tgctcttggt
      481 ttggtggtgc gccgagttcg cggcttttcg atgggcgcat ggcacttgag ttaatgaata
      541 acatagattt agagttcgtg tttgcggcaa tgacgtgcgc cggcttggcc cacattctga
      601 ttcaggggta tgaggctcta agggggaatg cgactcacgt ggggtggcgg tgctcgttgc
      661 gcagttagcg caagggcatt gcgcaaacat cgcgcgtgcc ccgggctgac cgccaattcg
      721 cggggttttc gcgctggaac ggcgcctgca acagggccgg gaagcatcgc aaaaacccga
      781 ttggaccgtg cgcaccgcga cggtggttgc ccgcgtccgc ccacgattga agcagctttc
      841 gacgttgccc caggagtctc cccgaatgcg aaattctcat aagtttgcgg cccgcatgtt
      901 ggtctccata gtcctgccct gcgccacatt cgcctgcagc ggctccgacg acaccgccgt
      961 ggacaagcag ccctctggac agatcgagat cttcagttgg tgggtgagtg gcggtgaagt
     1021 ggatgctttg aacgcggtgc tggacctgaa tcaagagaag taccccaaga ccaaggtcaa
     1081 gaacctcacc gaagatctgg ccgacgaggc gcggcaacgg ctcgaacaac gcatggccca
     1141 aggcgctccc cccgacacgt ttcaggcgaa tgtgggtgcg gatctcttca agtgggttct
     1201 cttcaacggc gtcgatgata aagagtccaa ggtcgagcca ctgaacgcga tcgccgaggc
     1261 gaacggctgg ttcgacgtct tccccaagtc cgtgacggac gcgctcagtc acaatggctc
     1321 gttctacggg gtgcccgcca acattcaccg cctgaactcg ctcttctaca ataccaagct
     1381 cttcgagcgc gaagggctga cgccgccgga cacccgggac gagctgctca ccgtctccaa
     1441 ggctttgaaa gcgaagggct acgtccccct gtgcatcggc agtgatcact ggtggaccct
     1501 ctcgctgttg gccttcgaga acatattccc ctcggtcgcg ggcggggact actaccttgg
     1561 atactggacc ggcaaaaaga cgccggacag tgcccaagtg aacgccgcgc tcgactatct
     1621 agaagagctc tggccctact tcaacgaaga cgcgaatagt ctcgattgga ccacgggcat
     1681 cgatcacatg ttcgactcga ccaacccttg cgcgatgacg gtcatgggtg actgggccaa
     1741 gggctatctc gaaaagaagg gttgggaggc gggtactgat ttcgcccaaa ttccgttccc
     1801 gggttcgcgg ggtaccttcg tgttcacggc cgacacgttc ccgctgccga agggggctcc
     1861 gaatcgcccc ggcgccgtgg cgctactcga gacattcgcc tccgtcgagg ggcaaattgc
     1921 cttcaatcgc atcaagggct cgatcccggt gcgcaccgac atcgatccga aagagttcga
     1981 ttccatcgcg cagaagacga tggccgactt caccaaagac caattggtga gtgcgatgtc
     2041 tggcttactg ccacaggact cgttcggaga cctaggtcct gccctcaagg aaatgctaag
     2101 ctctggcgat cgcgaaggcg tgctcaacgc gctcgcgaac gactacgcga tgttgaagta
     2161 gcgttagatc ggtggagcct cgacgccggt catcgagcgt cccccccact catcgggaca
     2221 cggcactctc caattatcaa tcgtgcttgc gagcgaagtg atgccatggc tatgccacaa
     2281 ttgacgcaca cccctgcggg gccgaccgaa ctcggcggta aatatcggct cctgctcgac
     2341 ctcggtcagg gcggtaccgc aaacgtgtgc ttggccgttg cccgcggtcc gagcggcttc
     2401 agcaaactcg tcgtgctgaa gatgatgaga agcaacctcg cggacgaggg cgagttctcg
     2461 aagatgttcc tgaatgaggc gcgcctctcc gcgcgcctca atcacgcgaa tatcgtgcag
     2521 accaacgagg tcttcgagca cgaaggacgc ccggttctgg tgctcgagta cctagaaggt
     2581 cagtcactct cggcagtgat gaaacgggcg cgtgacggca ctcggttctc cctgaacatg
     2641 cagctgcggg tgctctccga catgatgagt ggcctgcatt acgcgcacga actcgccgat
     2701 ttcaagggca cgccgctcgg gatcgtgcac cgggacatca gcccacagaa tgtgttcatc
     2761 acctacgatg gtcaggtcaa actgctggac ttcgggattg ccaagctctc cgagtctcac
     2821 gtcgagaccg cgacgggtgt gctgaaaggc aaggtccgtt atatgtcgcc cgagcaagtg
     2881 gtcgggcaga aggtggaccg gagaacggac atctttgcgg tcggcgtgat gctgtgggaa
     2941 gcggcgacca gtacgcgaat gtgggcgggc ttgggtgaca ccacgatcat tcatcgttta
     3001 ctgaacgaac ccttgccgcg cccgagcagc gtcaaccctg ccgtcgatcc cgagctcgag
     3061 cgtatcatcg tcaaagccac tgcggccgag cgcgagcagc gttacgacac ggtcgcggac
     3121 ctgcaacgcg acgtcgacga gttgttgagc aagcaaaacg tcagcgtacg tcagcgagac
     3181 attggcgcgg tcgtcaccgc gttgttcgaa gacacgcgcc agcggagccg ccgtctaatc
     3241 gaggctcagc tcgacaaagt ggcgacgctg acggatgccg agtacgccgc cgtacagccg
     3301 ccggagctat ccagcttggg cgtcacccgc accggacgaa cggagctaga gccctcgacc
     3361 acgggtgcct cctccaaagt ggtcgatacg cagccacgcg cattcggggt taacgcatca
     3421 ggcaagcgga aggcgctcgt tctcggcgcc atagcctgtc ttggagcgct tgtggccgtg
     3481 gggttcgcga gtggggtcgg gagcaagccg gtgcaagcgg tggccgcgcc tccggtctcg
     3541 ccggctgccg tcagcaacag cgtgcgcctt cgcatcacgg gctttccggc ggcagccaag
     3601 ctctacctgg acgagcaact cctgccttcc aacccctaca gtcaggacgt gcctcgggac
     3661 acgaaaaagc acgtcctgcg ggtcgtcgcc gaagggcacc gcgaagagca aagggagctc
     3721 acgcttgccg aagacagcga agtagtcgtt gcgctcggag cgctcccgca gagctccacc
     3781 cccagctcgg gggaaaaggt cgcctcgaac agccctgcga agaaggccgc gccgatggag
     3841 cccatcgacc ctcgacgtgt gcgctccacg ccgattgccg aggcacccgc caagcccaag
     3901 ccggaggcga actccgattg ttcgccgccc tacatcgtgg actccaaggg ggtcaaacga
     3961 tacaagccca actgcttctg agagctgaca ctcagggcgc ggattgcaga atgggcaggt
     4021 gcaccgcccc gcacatggct tcttcctcac tcgtctcccg gagaggaaag aagtctaacg
     4081 gcggaggctc gacgcgcagt cgctcgcact cgagctgaac gagcgcggcg caccacgcga
     4141 gcgcggggcc gtatactggg cactcctgca ccgcgggttg cttcgcgatg acgcccgcga
     4201 agcggccgag gtggccgtgg aggaacttgc gtcgcgcgtc ttcgacaatc tcgcaaccct
     4261 ccgcgtcccg tgccgttgcg agcgccgact gccgaatcag gagctgacta tagaactcga
     4321 gctctaccgc gatgtggtcg agcatttcgt gggcgttctc ctcgttcgga gtcaacccaa
     4381 aagcacggta gaatccggca atgtccgcga gatccttgcc tttggcggcg ccgcgcatca
     4441 caccatgttc ggtctcgtac agggagacct tggattgcac gccctcgaac agagcggcgt
     4501 aggcgacctg tacatggcgg aggccggctc gacaggcgct cgccagcgcg agcaaagcgg
     4561 gggcgcatga atcggattct tgcaactcga cgagcgtcag cactacctcg cgtgtgggat
     4621 actcgaggat caaagaggcc agggagaact cgagcgcgcc ggtgagcaga gcatagggct
     4681 ccaccggagt cagggagccg tttcccgccg tcgacttggc cccgaggcgt tcgtccgtgg
     4741 catgcgaatt cgtgagactc ggcatcaccg cctccactgc accgcgacat taggagccat
     4801 gttcaccgca atttcaatgg caaccaggac atggtgaggc tcttacgaga gcccacttcg
     4861 cctttaccac cttgccaaac cgctaccgcg agttggctct cgccagggct cttcagtgaa
     4921 gagcgccctg gaacctccag ggttcgcgta atcacgagcg tccagcggcc tgccttccac
     4981 gccgcgcgcg cctgcgcgtg accgttctgt tggacggcag aggttccaaa tccttcggcc
     5041 actacttcat ccacggcttg cttcggatag gactgcggat tgccgaccac ccgcgccgga
     5101 gagaaactct ccttctcggc ggcggagccc gaaggcgcgt ccttgaagtc catggcatac
     5161 atatccacgt tggcgttcgg gtacactccg ctgatctcgg gtttgccctt ctcttgatcc
     5221 gcttgatact gggccctcca atgtaggatg tgcaccggca gaccggggcc gcccatcatg
     5281 actggagtgg aggcgctgcc gttcgccgga aactgcaacg cgagagcgtc ggagaactca
     5341 gcgagcttgc ccgcggcgct cggctcggag tcactccaaa ccaccctgaa cgccgctcgc
     5401 ttgccgtcat gaacggcctg aatcacgacc ttctcggtgg tggtactctc cggccgcgga
     5461 ttcaccaaag gttgtgccgt cagggtgatg ctcccgcgcg gcacgtctgc ccagaacggc
     5521 gcgtctggat cgggctgaga caagtctgct ttcacgcgcg tgacgttgag ctgctggagc
     5581 tcgggggcag gcaaagcgtc cgatgcggaa ggcggggcgg agttccggca cccgaccgca
     5641 gccgtcatcg aacccagaaa aattgcgata gtgattgtct tcgacacgca gcacctcacg
     5701 gggtgttatt ccggatcgcg ccgagctccg cgtcgaacgg cgcacgttgg tgaactggct
     5761 ctgttacggg cacttcgacg atcagctgcc ccaattcgtc atagccactc gcgcgcccag
     5821 ccttgacctc gaagcgatgg atgatgcggt ccgtgctgcc gatcaggcac aaaaggccct
     5881 gcgctctcgc atcccgggaa aggtcccggt agcgggcgat ggcctcatgc acgcgggggc
     5941 caaacatctg gaccaggtat tcggggtcgg cgtggatcgg agggatgtag tagatattgg
     6001 gttcgaggcc caactgcgga tagtagggga gggcgaggcc tttttcgtgt accaaatagt
     6061 caacggggtt gtctttgcgc gccacggcgg gagggctaat gaaacccatg acccgaatct
     6121 tgccgataca gttgacgaca cactgtggct gatatccctg ctctacggcg gggaagcacc
     6181 cgatacactt ctcgctcgtg cgcgtgtatg ggttgtacat ggacttcttg taggggcaag
     6241 cgcggacgca ctcgccgtag cctttgcagc gcgcctgatc gatcagcacg atcccgtctt
     6301 cctcgcgctt gtaaatggcc ttgcgggggc aagcggcgag acacgccgga taggtgcagt
     6361 gggcgcacgt gcgcggcaga taaaagaacc atttgtcgtg cgtctcgccc gcgaggtgag
     6421 ccccggtatc cagggttccc cctgcgcagt cgtcttcccc aacgtttggg tacatccagt
     6481 cctcatccgt agggctgaag tggagtaccc gctcagtggg tggggcggct tcgaacagcg
     6541 tcttcccgag atactgaccg ttctggcccc agtcctggcg cccgagcttc tcgagaacgc
     6601 gaatatccca gccgagcgga taaccgcccc aaggcttggt ctcgacgttg ttccagaaca
     6661 tgtactcctg acccttgccg ctcgtccagg tggtcttgca ggcgagagta caagtctgac
     6721 aagcaatgca cttgttcaga tcgaagacga tggcccactg cttcttcggc cgcttgcctt
     6781 cgtaaacgta gtcgacgtca cggcgtaact gccagttatt tactttcgcc atgacggcta
     6841 tttcccctgt acgctcgtga agcgcccgcg caggtacagc ttcatcgcgt cggtttcgta
     6901 cgtcggtcgc agaccaagcg ccgcgggtcg ccagagcgcc tggcccccgc gaccgccgcc
     6961 ctcggcgcgg gtcatcctca cgaaggactc gcgtggggcg cctaccgggc aatgtacatc
     7021 ggccaggaag cccttggtca gctcttgccc gtacatgtcc ttgtgaacga gggactccgt
     7081 catgagcgtc ggtttgagcc aggcgcgcgt ggcagactgg tgggaaccga agcgatacat
     7141 cgcttggtag ttggtgaccg ggttgcgggc gaggccatcg acccgctttt cgtggccctt
     7201 cacgctccca aaggtcgctc catacatgtt gtgccacgtg cgagtgacgc gtcggggcgt
     7261 gcctggatag taacggacgc ggagcaacag gcgcgccact ttgtaggcct cggtgccggg
     7321 cttgaaccca cgatatggac gatcgattgg atcggcgtcc acatacacgt agtcgccgtc
     7381 ctcgacgccg agctctttgg cgtcgagcgg atgcatatcc acgtatccct cggtcacgga
     7441 cggcattcgt ttgtcatgcc gatagatgtc cccgaacggg ccgaacagca ccgagatgaa
     7501 atcggtgtcg accggagtcg tgtgggcgcc gtggcggtac ttcggggtgt gatagatgta
     7561 gttgtagccc ttgtccatca atggatggcg actcgccagc gtctgctcga cgctgcgctg
     7621 cacgtgccgc acctgacgaa cttccgtcga gagatcgtcg cggttcaagc cgtacgcttc
     7681 gggccccgtg gggtgcacgg cggggtgagg ccccgataca atcacattgg gctcgtggaa
     7741 ggtagaatcg atcggctccc gatagaccac gagattctcg cccgactccc ggaactcggg
     7801 ctcgaagcga tagaactcga gccgccccga cttggtgtgc caaggctgtt caccgcgctg
     7861 ctgctcgtaa gatgaagcgc gcgggtaagt ccgcacattc aacaaggcgg ggatgccctg
     7921 ctgggccagc tggtgcaggt tcttgaccga atagcccttc agcgcgaacg agtgatcgat
     7981 gatgcgctgc aggtattctt cggcgttgtt ctcgtcgatg aacttgaacg cctccgcgaa
     8041 gcgcgtatca ctgtacagct ttccgagcgc cttgctcacg ccggccaaca cctcgacgtc
     8101 cgcgcgcgta tcgaatactc gctggatctt ggacgtcggg aaggtctgca cgaaggggtt
     8161 cgtcgtgctg gccgtcatgt ccgggtagcg aaactccgcc caagagtcca ccgcgaagac
     8221 tacgtccgcg tactcacacg agccggtcca ccaccagtcg gagaaggcga tgaactcgat
     8281 gcggggcagc gtgttgttga cgacgtcgaa gtgccacttc gcgtttccga tcaccgagtt
     8341 gctgttgctc agccacatcg ccttcgtcgg ggtgggaacg tggcccttgc cagtaaacag
     8401 cttgttcccc tgacgcaacg ggcgatcacc atagttgaag tagtgcagag actcgtaacc
     8461 agagtacttc cgcttggtga cctcgccccc cgccgcgagc tgcaggttga aaggatcttc
     8521 cgcgacgtag tagggcatcc cgccgaagat cgacgtgcgg tagttacccg aataactccc
     8581 cacattcccg ccggggaagc cgaggttgcg cgtcaaagcc gccacgagca ggattgctcg
     8641 gtctttcaga tcggaatgaa agaactgatt ggctcccatt cccactgcga tcgtcgtcct
     8701 ctcgggattc tgggcgacct gacgggcgag ggactcgatc gcgctcacgg gcgtgcccgt
     8761 aatccgcgcg acctggtcgg gggtcatgtt ctcgtccaga tactgggaca ggagcgagaa
     8821 atgggttcgg acctggacct tctcaccact tctcagctcc acctcgagag acgcacttaa
     8881 agcgggtgag gtgttgcgag ccttggcccc gacttggtcg cgcgtcaccg cggtcaatct
     8941 cttcgtgcgc tcgtcgagga tgagataggc cgcaaagtcc tggctcatcc cctccgaagc
     9001 caactgcgcc ttgtgctcca gggcgggcgc tggcttttgg tcctgaggat agagagcgag
     9061 attcgtaggc cggtggctga cgtaaccggg cagcgcgctc ggcgccacat cctcggggcg
     9121 caggggctcg agcgtgtcca tccgaatcag aaacggcaaa tcggtgttcg tcttcaccca
     9181 cgccgggtcg tacagcttct tggagatgat gacctgggcc ataccgagcg caaaggcggt
     9241 gtcggtgccc ggccgaatca ccacgacctc atcggacttc gaggccgtcg cgctgtactc
     9301 cacggtcacg gccacgacgc gggtcccctt gagtcgcgcc tcggtgagcc agtggctgtc
     9361 tggcatcttc gtggtgatcc aattcatgcc ccacacaatg cagagcttgg agtgctcgac
     9421 cgtaaagagg tcgaagtcgt tggtctgggc gccggtcacc atcggatgac ccggaggcaa
     9481 gtcggtgtgc caagagtagc tatcccagcc gcgggcccca tgcgcccggt ccgcgcctac
     9541 tccacggatc ttgccgtccg ccaacgcgag caggttggcg aatcgataca gaccgaagat
     9601 gcgcgtcacc ccgagcagcg ccatcccgcc gcgcactttg atggtctggg tgcccgcccc
     9661 gtctagggcc tcgatcatgg cgggatcgta gccttggaca ttcaagcgct gctttccggt
     9721 ttctcccgaa taggcacgcg cgatgttgtc caacgcacgc gcggagtagt catacgcttc
     9781 ctcccaagtc gcgcggaccc agttgtcctt accgcgctgg aagtacttcg gatcgggctt
     9841 accggtgctc gggtcccgcg gaaaccccgc atcggcccaa gctttgaagc cctctcgtaa
     9901 catgggcgcc ttgacccgtc gatctccgta gatgcgacgc accagcgcga gtcccttctg
     9961 gcagagccgg ggatcccagc ggtgagacgc cttgttcccg tagaggtctt cggctttgcc
    10021 gtagccgtag gttgggccaa tccggacgat gatgccgttc ttgacgtggg cgcggagcag
    10081 acaattgtga gtgtcgttgg gtgcacacag gaaaacaaag gaggagtccg ccttgaagat
    10141 gtcgcgatag gtgctctccc agtcccgact gggataactc gtgagcggat tagcgaagct
    10201 gagcggcttc aagaagcgca ggggggcggc caagccatgc gcggtagcga gcgctgcgcc
    10261 accagcgagc gcacggatga agctacggcg cgggaaacta ggatcagaca ttgaaactcc
    10321 gttgggctca gcccaaaccg cggggggaca ttgtcatcgc gcgcacgcct gcgggggcgc
    10381 actgctgaga acgctcttgg gggacgcgtt gcacgcacca agttggggac atcgccgctt
    10441 gatggaccga gcgacgaccg cattcagatc gacgttgtcg cagcccgcat gttcgagcgt
    10501 gtagtacagg attcgctcgg atattccgag ccagcggaga cgcgccgagg aatcgaacat
    10561 tttgactgtt tgctcgagcg tcgcgagtcg gagccggatc ggagcgggta tgggtccgaa
    10621 gcgcgcgggg ctctgcgcgt ggacgaacag cacatcgacg cactcagcat cgccgcagtt
    10681 caggctctcg tgtcgcgcgt gatggacgga gctcgtcaag cccaagacca aaccctgccc
    10741 ggcttcacgg aagtaatcgc aagtccgccc gtgcagcgct cgcgaggcgc atcccttacg
    10801 ctggccccgg tagtgtggag gatccgactg gtccgtccag gcgaaggcga aacaaggcac
    10861 ttgcagagcc ggcaggatgc gggtcaggtc gagcacggtg agatctgcgg tgtagtattg
    10921 gcggaagctt ccgcctagac gttcgctgtt gtcgaagacc ctccgtagta cgccctcagc
    10981 gtcgatatcg agtgccgcgg gtagcagctc cgtctcgctt gcttccgcgc tcgcatccgg
    11041 aacgaacgca cgcaacgcgg cccggcgtgc gccacaccat ctgcgagcta aatcggaaat
    11101 agccgtgttc atagagccgt agtctcgatg caaaaagatc cggacattcc ccggagtcga
    11161 tggaaatcga ctcccttccg aagcgagtcg agaaacaata tcagtcgtgc gctcgccttt
    11221 cccgagaaag gatttgcgat ttgatacatc tcaatttttg attggccgcg cgtgcgacta
    11281 aatgtctcgg cgcgtgcgcg gcaggcgaag tgcgaggacg gcggtggtgg atgacgagcg
    11341 tcagtcgccg tgcaataggt accctgcgcc gttgaccgcc gtcactcggc gcggatactt
    11401 cacttcatga cctcggcgac aatcaagcag ctcttggcgc gaacaccggt cctagagaag
    11461 tgcactgagg acgtcctgga gactctcgcg gcgcatgcca gcagccggga acactctcgc
    11521 ggtgatgtct tatggcaggt cggggccgag ccgacggcgc tggtggtcgt gagcaaagga
    11581 ctcatcaaga tgaccagagt gggcggcgtt gggcgccgct cgctgtgtgg atgtttcggg
    11641 cctccgcaat ccatcgggga cggagtgttg gttcgaggct ccccttatcc tgcgcaggct
    11701 gccgtcgcga cgccgcgggc cgcgaccatc tgggtaccgc gagggctatt cctggaagcg
    11761 atgcagcgtt gtcctgagct gggcgtatcg atgtgccgac acctgcaagg caagctcatg
    11821 gcgctgcacg acaagatcga ggtgcttagt tcaggtgacg tagaagcacg cctcgccacc
    11881 gcactgctga agttggccga ccaatacgga gacgatttca tcgacggtag tcacctggtc
    11941 ccggtggctc tcagccgaag ggagctcgcg gactgggtat cgacgtcggt cgagacggcc
    12001 gtgcgcgtca tgacccgctg ggagcggcta ggtgtagtaa cgaccacacg ccaaggcttc
    12061 ctcattcgct ccctggagga gctgaagcgg gctcgcggag acgttgctgg tgaatccatc
    12121 tcacctcttg cggagtagcg atccctcctt cataagagcg aaccatgtct tgggacggaa
    12181 tctatatctt cgaaagtgaa tcggagagga actgctggct gccgatggcg gctcggcgag
    12241 ccctcgacat ggccggaatc catgtttcac tggagccgtg gaaggggctc agcgcggagc
    12301 agcgccaagg gttcatcaat ttaggccgcg cgccaacggt agatgtggcg gcggtgggca
    12361 ggcgggtcga agagctgggc ctcgcgtcgc ggcccgagca gaagtggact gagcccggag
    12421 ccgacgaggt tccgcgagac gttcaactag cgctcgcccc acaattgacg atctcgaagg
    12481 cggagtggtc gcagctgagc cctctcgatc gctacacgct atataagctt gcctcccgtg
    12541 aacgcacgga caaggctaag ttagcctggc gagaaatccg agctaggagc tttggcaagt
    12601 agccagtcca ctcccgtcgt ccgttcgcgt ccacaggcgg agagagcggg cgcgaggggt
    12661 cgcacccgcc tagtgttcta acaccagtgt tagggccttc agatgggctg ctcgcaaagc
    12721 ccgatttcac cgaggctttg gctactaacc tttcttgaat cctctgacga tggcgaccag
    12781 cccgtttacg acgtctggtt tcgcgctgag atccggattg gcgggcatgc ctgggctctt
    12841 cccgatcgcg gcgccgccct ctaaaatggc cttcttgatc tgttcatctg tcacactgtt
    12901 ttgccaagcg ggatccgcaa acgcgcgtgg cttgggatcg agagcggctg ctccaggccc
    12961 gtcccctgcg cccactgaac cgtggcacac cgtgcacttg gtattgaaga cagtttgggg
    13021 ggactgggcg ggagcttcgg cgcccttggc caaagcgccc tgcgcggccg gctcggggac
    13081 ggcgtagctc ccgctgctct ctgatgagtt cttcgagcag cttggcagca gggcgcaagc
    13141 catcatgatt acgaaactcg tcttacgcat gtgacacgta tctcccatcg gctggatcaa
    13201 taactctgtt cgaaaagtta ctctgtccgg gcgactgctg acgaccgaat accctatcgc
    13261 cgatcaggac aacgggtagg ttcggcatta tgccagcatc gaccctcctg agaagccgaa
    13321 tcgaggccgc tcgctccgcc catttgatag gtgtcaacgc gtcgcgcgcg cccggcgccc
    13381 tgaatggccg ggcgctccgc cactcggggg tcgaccttta cgatccatac gacgcgggcc
    13441 gttacgattt gtgaaccgga gtgctctcat tgcgtaggga gcaggtccgc gcgatggaag
    13501 ctgcatctac cccgcgacca cttcgcgcgc acgagaatcg agctggtgct ggagtcgttg
    13561 ctcgtcctcg gcggacatgg tgtcgaattg gcgcggcagc atgtcgcgta gatcgatcag
    13621 cgcgtcgagc acggcttctt cgttgactcg ctcgggatgc tcgcccactt tcgatcttgc
    13681 gctggcgacg cgagcggcgt tcatgaggtt cttggcgagc gcgctcaggg cttcgaattc
    13741 gagatgggtg ttggcgtcga aggcgttgct ttcatcgaat ctttgctcac agaactcggg
    13801 aattttgcgc ttggtgaccg ggcgttcatc gacgctggtc tgttgcgcgt acttgagcac
    13861 gtatccctcc gcgtagttat tggggatcga gggcaacccc cagagcgccg ggatgagcgt
    13921 cggaaagcgc acgggcaggc gatcgaggtc cgaatagcga cccttggcga tcaggggcac
    13981 ggtcgtgatg cccgccgctt cggctagggc gcgagtttgg gcgtcgctca gaaacacagg
    14041 ctcggccccg ctcgcctggt gcaccatatc gaacaccgca tagtgcaggt ctggcgcata
    14101 ccagatccca gtctggacgg gcaccaggcc cgcgaccggg gtgacggagg gttcggggta
    14161 tgagccgcca aacaactcac catagagcca aactacgccg gttgggtcga ggtttcgctg
    14221 aatggcccgc gcggcgtcgt tcagcagtgg gcgtagcatc tgccagccga agaagggctc
    14281 gtcgggggcg agccacgcct tgcgcttgcc cgtgcgcact tcgtccgcat tggcacccac
    14341 gacgagctgc gctccgtgaa tcttctccga tgccacccag tcatcgcgag aggcagacgc
    14401 ggtcccacca ctcgagatct tggggaaagg taaatgctct agggtcccca taacggccgg
    14461 gtaggttacc gcgggccgcg gcggtcgcaa ttcggcgacc gtcacccgct tcggttcttc
    14521 ggccaaggtt gcagcggaac gagtgggatc gagagaagtt ggagccaaac cgttggccca
    14581 aaaacacgag cgccgcgctg aggaagttct ccgcgcggcg ctcgcttcga ctcagactac
    14641 cggttgcgaa tcaggggatg ggagtgaggg tgacgtcgtc cacgtaaagg tcaccgccgt
    14701 ctgcacccac gaacagttgg agcttctcca cgctgctgca ggcgctcaga tccaccgtgc
    14761 ccgtgagttg cttccagctg ccgttggtca cggtgtcccc gctgagccag ggatagctgt
    14821 cgctgccggt ggcgttgcag ctctggatgg tctggaactt gacagagccc gaacccgccg
    14881 cgaggttgcc aacggacacc caagccgtgg cgcggtagcg cttgcccgcg gaaacaacgg
    14941 gcttgatgtc tcgcgagagg gcgccgttgg gctgcatccc agttcccttc aagctcgagc
    15001 tacccgcgtg cgacgtggtg ttcacgaccg caagtgacgt gtaaccccag ccaccccagc
    15061 cgcctggtcc ggactcgaac gtgccgtccg tgatgaggtt gccggacgcc gcgctgttgt
    15121 ccatgacctg aacgtcgtcc acgtacaggt ccgcgcccgc tccgccttct acgaacagct
    15181 ggagttgcgt cagcgtgcag ttcgggaccg cgatcgtgcc ggcgagttgc acccaatccg
    15241 cgctgctcgc gacgttggtg ggacccgcga tccaggcata actggtgctg gtcgtgccat
    15301 cggcggcttt gcaggtagtg gcttgggtga cgttgatggc tttggccgtt ccgtctggcg
    15361 atcgtacgga tacccacaag ctgaatgggt aactgctgcc ggccttgacc acactcgtga
    15421 gatccgtagc cgcggggtaa tttcccgaac gaccgctgac caacaggctc ttcgccccgc
    15481 cgtggacgcg atcgctggcc gcggtgagcg tggctccgcc ccacgtgaac cagccgctcg
    15541 tgcccgactc gaacgttccg ttcgtgatca cgtttctagt cgtcacgccg cggaccgagg
    15601 cgtggtcgat atatagatcg accccggccg cgggcccttc gagccagaac gcgacgttgg
    15661 caagctggca atccggcacc acgaggtctc cggcgaactc ctgccattgg ccgtcactca
    15721 gcgtcttcgt gttttcccaa cccccgaggc gcccgtacgt cgtctcaccg ccgacgcact
    15781 ggagtgccgt cgtcacgtac gccgccgaag ccgcgccgcc gaccgtggcc cacaagctca
    15841 ccttgtacgt tctgcccggg gcgaccgcgc tggtgagggt ctgaacgagt gggctgttgc
    15901 cattgcgagc ggtcatcgcg atcgaatgcg tgcctgtgag agcgcgcgcg gtcgtgttcg
    15961 cgatggttcc gccgccccag ctcgaccaac ccgccttcgt gccgagttcg agatcgctat
    16021 ccgtgaggag gttggccccg gttccgctcg gcgctgcgaa ctggagcttg tctacgttgg
    16081 cgccgccgcc gttgaagcgc acgaacacgt tcttttgaac gttggtgggc gcccacggca
    16141 cgctgaccgt cttgctcgtg ccccagctgc cagtcggggc gagcggcacc gtggctaccg
    16201 gagggctgtc caggctgtcg aagtgaaccg tcaattggac agggctggtc ccgccctgcg
    16261 cgtacgtgac attgagtgaa tcccagttgt tcctgaatgc cacctggtcg aaggcaaacc
    16321 acgaaccgtt gctggcgtaa gcgacgactt caccggctgg cagcagcgca tgcgccgagt
    16381 cgtactgctc ggcttccagc gtaatgccgc tgtcgtcgcc ggtacggggt tgcggaacat
    16441 aggccggaac cgggcaattc gtgccgcccg ccttgaagca ctccacccaa gtgagcgccg
    16501 ggcgcaggct gccgtccgtg cggatcagat aggcattctc ctgccacatg ttgccctggc
    16561 ggtgccccca gtgggtgacg ccgagcacgg acctgttcga ccagaacgcc gtgaatagct
    16621 ccttcatgcg aatggcttgg cgcgcgtcgt ccgcaaagtt gaggtcgagc tcggtgatgt
    16681 agatgggcag cccagtcgcg gcgaaagcat tcaagttcgc cgtgagcacg gacagctcgg
    16741 gcgcacgctc gtagaagtgt gcctgctcgc cgatgccatc gatcaagccg cggtcactca
    16801 ggactttgac caccttcaga tagtcctgcg tggtgctcgc catcgtcagg atcgagtagt
    16861 cattcaaaat gagctcggcg ttggggaagt gggcccgtgc catttcgaag gccttgatca
    16921 cccaatccca acccgtcgca cccgcgccac ccagcgccgc agcatagcta ggaggcgcat
    16981 gcagtggctc attcacgacg tcgatcatct gcacgtcggg gtagcgctcg gccagcgcgc
    17041 tcatccattg ctcgagctcg gccagctgct cctcggggga gagcccatcc agccagttcg
    17101 gctgttgctg gccccaaacc aaggtgtgca acttgaacgg gatcttgttg gccttggcga
    17161 agctgtaagc cgcgtcgaga tcagtccaat tcatctgatc gcgagttgcc tctaccgacc
    17221 cccatttacc ggcgttaccg ggggtcagct ggttgaagtg cgccgtgaag ttggtgtagt
    17281 ccgccgcggt cgtggagatc gagccgaaga acttgttgtc gatggccacc aacacgcgct
    17341 tggtctcgcc gccttgattg ccactcaagt cataggcggt cgcgctgtag cgatggcgcc
    17401 cgttcagcgc cttgctgatg ggcacattga gcgtgtacgg agcggtggtc gccgtgccaa
    17461 tcggcactcc gtcctgagcg aataccacct tcgagaccgc gacgttatcc gtggccgtgg
    17521 cgctcagcct cagtgagccg gccgacgtga agaagtcccc gctcgccgta agcttgagcg
    17581 tgggcgcggc ctgatcacag gcaactccat tattggattg aatataggcg ttgagtcggg
    17641 tgtaggtgcc ttcgaacttc agctcgaact gatagtcatc accatcctcc agctcgccca
    17701 aggtcttctc gcctggcttc agcagataac cgttctcggt ggcggtgaag gtgcccttgg
    17761 agctttgaac gagctgggcg ccaccggcgt cgatcagcac cgagaagttg gtcgaagtcg
    17821 cgccgctcga gttcttgacg cggattttgg cgtgaaattg cttcttgttg atgccgtgaa
    17881 cgtcagaggt caccgagaag ccacaatccg ctccttcgac ggcctggctg gccgcaccca
    17941 agtcggaatc accaccttgt gcggtagaac atgcggctat tgcgagcggc aacaggcaaa
    18001 aagagaccgt tctcagggat cgccgcgcga cagtattcac ctgtcgcagg ccactatctg
    18061 cgtgtgtcat tagttgttgt cctgcacaac gagcatcggg ctcgatggcg cgaccagcat
    18121 ggcacgcgcg catgcgctgc gctgcaaact tttatgtatt cgatagcttg tcgatctgag
    18181 aatcgatatg atctgtttct agcaactcga gacatcgttg cattcgagcg gctcttctcg
    18241 cgtgtctcgc gaccctgctc aaaacaagac cggtgcccgt gtagtcaacg gaatgcgcaa
    18301 gtagaacccg cactttctcg ggcaaaatgc cccgctctgc gcgagtcgtg caaacgagcg
    18361 caaggccggc ggcgacgccg gcgccggcgc acggtcagct acggtgctgg caattcggat
    18421 cacaccgaat gcgaccaaca tgacagatca caatcgcgct cgggtcgcgc ggcgcgcatg
    18481 accttcagca tcgagcatgc ggggcagctg ggactgggga cggcccgatg taaagggcgc
    18541 gaagccagcc ccagctgccc ctgagcgcag ccggctgcgc acgccaagtc gtcgcgatac
    18601 cgcgcaagcc gcacgcgccg gcaacacggc ggctcaaagc gcttcgagga acgcgaccaa
    18661 gtctcgttct tcctgtgccg tgaacacgaa gccgagcgcc gtttcgtagt gccgaacgac
    18721 ctcgagcaag ttcggcgcga tgccgttgtg gaagtacggc ggacgcgcgg aaatgccgcg
    18781 cagggagggc gtcttgaagg cgtcgaggtc cgcccagcgc ccgcttcgga gcgcacgacc
    18841 gggatcggtg gtttggcgct cttccaaggt agtcttgttg cgcagagtgt agagcggcat
    18901 gcccggtttg cggaacgccg accgcgacgc accaatatcg aataggcgcc cgctcgcatt
    18961 gctgccattg ttggcggcgt tgtggcagcc gctacacgag ccacccccgg ggttggcctc
    19021 gttgaacagc ttttgaccac gcgcaatctg ttggcgccga cgatcggcgg ccctcgtcgt
    19081 gcatgaaccg ggcgcgagct cggcccaggc atcgaacaga tcgaagcgcc ccgcgaccgc
    19141 cggctgttgg gacagattct gcgggccacc gcgcgcgccg caggagtcga gaaaaccaac
    19201 cccgaacacg tattgttgag cgaaactcaa accgagctcg taattgacga tcgatgcaat
    19261 ggtttcgggg ggtgcggcgg ctccttgttg agcacccgtg atgtttcccg tggcttggct
    19321 ggttagcccg gccgggatat cacccgagcc attcgtgttt tgatcgtgcc aaccgatgtt
    19381 cttggcaata tggaagtttg cggtggcgag tgggcgacgg aacgcttcga agcgcgtgag
    19441 gctgccgccc gcgccgagcg gatcgtccac ggcgatgatc tcgtattcgg cattggccgg
    19501 aacggcccca cctctgcgaa acagaccctt gcgcaacatg ctgtatgccg tgtagcgggc
    19561 tcgaggcgtg gagacatccg cgttgggact atcggcgtcc aatagattga agattggatc
    19621 gttgccttgg gtgagggcga atttgagctc tacgtcgatt gggcgtacgc tccaacccgc
    19681 ttgtggcaag tggcaactct cgcaggatct cccgttttga ccctggggaa cgtgaaactc
    19741 attctccaga tccacgaatc cggctgtgct gaatgtcgcc gcggttccgc tggcgtctgg
    19801 cagagggaaa ttgttgggca gtcgggagat tgtgagttcc tgctcgctcc gactcaaggc
    19861 atccgtcagc gggtctgcgt cggccgcatc ctccttcgcg gcgctacacc cggctgtgcc
    19921 gaacagcaca gcccaagcaa cgaccttcgc tcgctcgaca ctccacgtcc atgctcggtg
    19981 gctcgatttc atcatgacct cgtggccgcc gagagtaccg aattcgccag acccccgcga
    20041 tggtcacctg cttgtggcgc gcggcttctg ggccccggcg ccgtcgcccc cggatctgca
    20101 ggccccgacg gcccgtgtca ggccgctctg tccccacggt ggcgcgcgcc gcagatcatg
    20161 actccgcgag tgcccaagat gctcaaaagc ctgtcagtgg cgcggcaatc gtacccggtg
    20221 gaatatcgcc tcgatctgcc gcccgatccg tcttgagcgc cactgcggtg agcgtgccgg
    20281 cggcgagggc aacgccgacg ccggtccaaa accaccagcg tgcatagact ggagtgttgc
    20341 gctgcagggg ccattggagg tgacgtttct cccgcgcggc aagcacgatc tgtgagtctc
    20401 gtggctcgta accgtcgctg cgcagctgga cgagatgact gccggcggcc aagcgcatct
    20461 cgagcggggt ttggcctacg gtattgccgt cgaccgtcac ccgcgcgcca gggacgttgg
    20521 aggcgatggt tacgaacgcg gcgtcgtcca ctcgcggcaa ctcgagcgcg agcgtggcga
    20581 caccttgggc gggaatttgg atcgtacgcg tgatttccga atgaccgggt gcgctggccg
    20641 tcacctggta aaacccatcg tccagccgca gggactcgga gggagcgctc atgacgagag
    20701 agcgctcacc cactcgcagg tgagcaccgg cgacgttgca ggaaatccgc aactttccga
    20761 ctcgttgttg gagcgtcgcg aacagcgctg aaagctgcgg cactcgtcct ttcagctccg
    20821 gtgtcgctcg gtccgagaaa ctttcgagat acatcagcgc gtcagcgtgg cgcccgaggc
    20881 cttggagggc acgcgctcgg ttgtaatcga ggattgggct agactcgatg gccgcggccc
    20941 gttcgtaagc atcgagagcc gccttgaaac gactctcgtt catcgcggtg tcggcttcga
    21001 gtttgagcga ttgcgccaga cgatcggtca gctcttgggc cgcggaagag tgggccgata
    21061 gcatcagcgc cgaaatagcg gcgcagtgtg cgagcagacc aattccgaag cgcgaatcga
    21121 attcaaaggt gtaacgggtt accatcgttg ttactaggag cagaaggggc tggccgcgtg
    21181 gggagggttc gctcggggct acgccggacc ccatttctgg ggaatcgcac cgcgggcgcc
    21241 gaagccggtg tggtgggaac tgagctcgcc gagagtggag gtgacgacgt gggctgagtt
    21301 tccggccctc tgtcgggtct tgtcgaacca gtcgagtcat tcttcggcgt tgcgaccggt
    21361 gcggcgctcg agtggggctg aatcgtggaa ctcagcgggg ctgagctcgc gttttgactc
    21421 gtgccaacgc gggatctccg ccaagcctcc actagtgata tcgatacgag gataaaggcc
    21481 aacgtggtga cggccgcaac cagccgcctt cggagtggac ctcgatgggt cgcacagagc
    21541 accttcgaga tcgccctctc cttggtgcct tccagtgccg gttggacggt agccactatc
    21601 gtcagtgtgg tggcgtctgg aacatgattg ttcaccacat cgagggtggg cggagagggc
    21661 gagaccggtg cgcttctcga gccgccctgc tgcgaacgca gagcgctggc ctgcgcattg
    21721 tcgaggggca cgcgactcag cggctcttct cgacgcgcaa cggcggctgc gagctgtttc
    21781 agtcgctctt cgctgatcgt gaaaggcgcg agcagctcgg cgagttcaca agccgtggcc
    21841 gggcgcgcat tcgggtcttt ctgaaggcag cgttcgatga cgttcacgag ctcgggtggg
    21901 atttgcgcgt tcacctcgcg cagtggccgt ggctcttggc tcgtgatcgc gagcaagatg
    21961 ccgcccggcg tcgcggcgct gaaggcagga aagccagcca gcaactcgta gagaatcact
    22021 ccgagtgccc acacgtccgt gcgcgcgtcg atcgcgaacg gcgtcaggat ctgctcggga
    22081 gacatgtaca ggggcgtacc gagcgagaga tcgggctcgg tgagcgctgg atcggcagca
    22141 ttcagctcgc gagcgatccc gaaatccacg agcttgatgc gcagtgaatt ctcgagctcc
    22201 gtgacgaaca gattctgcgg cttgagatca cggtgaacga tgcctacctt gtgggcagcg
    22261 gcggcaccgc ggctcgcctg acacacgaaa gtaaccgcct cctccgtgga cagcgcacct
    22321 cgggagctga tctcgttgcg cagatcccgc ccttgcaggt attccatcac caagaaccac
    22381 tcgcccgtag gtaacagatc agcgtcatag gcttgggcca cataggggct gtggatccgt
    22441 gccaacgcgc gaacctcgcg gcggaaacgc tcgccgagtt cgcgtgtagc cctctctggc
    22501 aataggatct tcaatgccac ctggcgatcg agtcgctcat ggtgcgcgag atacacgacc
    22561 cccatgccac cgctcgccag ccttttgacg atacgatact tgccgtcaat cagggcgccg
    22621 gaatcgaaca tcaatctgtc cgactaacga ctgcccacgg cccggacgtt agtggtcacg
    22681 gcgtgctcaa cttgcccaac ccactgtcca aggcgaattt aggccctcca cgggttccgg
    22741 gtccgcgtga gcccggacaa gtccgtcgga caggagctat tgggcaaagc cggtccgcga
    22801 gagtcgctct gctcatcggg acggccctcg gtttgggttg tgagcagccg accgaggtct
    22861 tcgtcgagat ctccacggac gctgattgcc gcgatgtgcg tgacacgagt ctggcgagcg
    22921 gcagactgat cgagatcgag ggcaaagtgc ccacgacggt gacccagcgc tgcgacgagc
    22981 agacgggccg gatcggatcg attgtggtgg tcccatcagg tgacgagaac caggaattcg
    23041 cgttgagtgt cgtgacgggg ttcaccaaga gcgcgcaaga gtgcgtgaga gacggctacc
    23101 tagggggatg catcgtggcg cgccgagcga cacggtttct tccccacgag cgcttggatc
    23161 tacccatctt gatgcaagcg agctgcatcg acgtgccttg ctccgccagc cagacgtgtc
    23221 gcgacggccg ttgcgtgagc gctactttgc ccgatccgcg ctcctgtgtg gaccctgcgg
    23281 gttgtccgcc ctccggcgct agaggcggga gcaccactgg ctctggcggc atgagccagg
    23341 cgagcggggg tctcggcggt gcgtccagct tggcgggagg ttcgagttcc ggaggagcct
    23401 cgagttcagg gggagccttc agcgtagcgg gaacccaaac gagtggcgag ggcggcgccg
    23461 gcgccagttc gagtgatggc ggcgagccca gttccgacgc gggcggcgct ccggaaggtg
    23521 gaggcaatca gggcggaaac gagagcggcg gacaaccagg tctcggcggt tccttggcaa
    23581 acggcggcag ctttgggggg acgctggtct cactcgaggg tggtactgct ggctccacag
    23641 gttccgcagc gggcgggacc accgtcggcg ggaccacact cggtggctcg caaaacgcgg
    23701 gcacatcagg ttcctcgagc ggcgccagca gcggcggagc atcgaccgga ctcggcggaa
    23761 acacctcaat cggtggcacg caatcgagtg gtggcacgca atcgagtggt ggcacgcaat
    23821 cgagtggtgg cacgcaatcg agtggtggca cgcaatcgag cggtggcacg caatcgagtg
    23881 gtggcacgca atcgagtggt ggcacgcaat cgagtggtgg cggggtgccg atgagcgttt
    23941 gtggcaacgg acttgtcgac gtgggggaaa cctgtgatga cggcaattcg attcgcgcag
    24001 atggctgcgc gcagtgctcg gcgcaaatcg gctgggcctg caccggggtg cccacgcgat
    24061 gtagcgtctt gcatacggcc agcttccaag tctcgggcaa tgacaatatc cctgctgacg
    24121 ggcttcgcaa ttggttcgcg gcgctccctt atgattcctc ctggtatctc tccgtcgaga
    24181 ttgacacgag cctcacgaaa ggtacccagt tctgcttgtc acgtggcgat tggtatgccg
    24241 acgcctattt gaagttcgcc ggcactcaag ccggcggatc catgagctcg gggatttggt
    24301 ccaaatggtg gcgaaccaaa gatggattgt ggtcccttcc cgacgtgctg cctcatctga
    24361 actcattcgg tgccgcctgt gacctcgacg acttgcaagc cttctgcgtc gagtcgttgc
    24421 tcgggctcca agagatcgcg gtccttcccg gcaacgctgg ctggactgaa gcgtacaatg
    24481 gcgggttcgc tccgctcggc tcggcttggc aagttcgtct ggcgctcgct ccttctcgac
    24541 aggaagcgtg cggcttctga accagcagct gcgcggttct gaccgcgcga gttcttcagg
    24601 ccaaaaggtc cgtcccctag actcatcgaa tcagctcacg agatttacgc gaaccgaacc
    24661 gagcgaggcg aaaccgaaaa gacatccagc tttgcacctc gattgatggg atgtgcctgg
    24721 attgagcgaa gtcgggtggc tcga
//
LOCUS       DASTKU010000784         6797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1941821, whole genome shotgun sequence.
ACCESSION   DASTKU010000784 DASTKU010000000
VERSION     DASTKU010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941821"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..1726
                     /locus_tag="VFQ61_27445"
     CDS             317..1726
                     /locus_tag="VFQ61_27445"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958272.1"
                     /translation="MLSGGTYPRLLILASWISVFALAGGCGGGGDDAGPSGGTDTETG
                     GTGDSPGSGGNDASGGRSSSNGGTKPGGETGGTTAEGGAAPGPEGGAPPVTVSPDPDF
                     EGIDPTEVPLEATPGCEGGFNPESGALSLDVEETSHFLLVDAFEGNVRANGQICSAED
                     GTPALAEALKKLTIVGSDDADRVILDMLDGAFNPELLAGEDALHVDLGAGTDSFSIRA
                     TREDDQVTCDGGPSPTRNLVTFGKADAAALTLDHAESLLVSLGPGNDAFLGYASDHPC
                     TLHIVAFGGDGMDLLQGGTQNDVLNGGEGSDEFEMGPFRDGADLINGGDGDDLVSYAQ
                     RRSDLRLKLCSAPDLTGCAEETCKCEALNGETAEADRLVNIEDADGGDGDDIIIGDEN
                     PNVLVGRGGADRIDGMGGTDQIYGELGDDTINGGAGDDILIGAQGKNFVDGALGDDIC
                     FFAPGDKPLANCETRIDSL"
     gene            complement(1815..2822)
                     /locus_tag="VFQ61_27450"
     CDS             complement(1815..2822)
                     /locus_tag="VFQ61_27450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958273.1"
                     /translation="MSRVLVIATALMASAGWSHVVSAQTRLGEASKSNLPNESAVPAT
                     SGADAPSSPAAPAAQNPAGAPNSAAASAPAASAPVAGPNSTIVPAPTLTAPASPPAAR
                     PAASAGANPTHVVQSAGELDVDQPEEPTDGSLGRFQKHGFVSVGFKSSLVKSSGFDPY
                     ASSDVLGYVSFNAGHTVFVSGPLSLVGAFGIDHGARNDTARGADTSLRVTRFELLPEL
                     RYHVLRQGYAFGHIGMGLSRVASTLDDRVTEQQFERKHLTFTGEIGAGFAYAVASSRS
                     AHKRTARLWLIAEGGLLYFPALSQALTNDSGVPARSRDIELPELALTSPYYRLAAALS
                     F"
     gene            complement(2823..4427)
                     /locus_tag="VFQ61_27455"
     CDS             complement(2823..4427)
                     /locus_tag="VFQ61_27455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958274.1"
                     /translation="MTELRAPGRRFWAFGGMAWACLALLGCGERKAFWDLPATSGEPI
                     GLRGAVAIPDLPRNELLMLTSPGQGELNVTRLPVGQNIVAAVPSRDLEHLYVLTRGQQ
                     RRVNEADEKPRLSVFKTGVAPALEQTYSLEDPAQKITLDPQGDWAVVYDSGGVVVNVN
                     EFVLVDLAHPDRAPIPKTIRSTGGRPEQFTFTTALQVPGSGPHRFLVVETDQDVAVMD
                     LEAPEAPEVTAPVPSTTLPGASQGKPAHPAQVVYVDASEETPAYLAVRFANDASVMTL
                     ELAEGATSAHAFSLRPNLLDAGDVASTIDFVQTDHGLRLAVLVPNRSQAVLIDPTTSR
                     SEKVQFGAKFAGMARITGLSGEDEGTADVALLYGPGTSSIAFWRLGDAGAAPYASFDL
                     YGTSVPVASIMNVPGNEYGYMKLLLSSDGVSFSLLDLKSRLSYAMQALQGFNVSFSPD
                     GERVWAYPQYSPRFARLNLSDKHPTSYDVELPVTRVFEVNRADGGKSALVLHGGSGDW
                     GVTLFDADRPDSAQTTFKSGLILEGI"
     gene            complement(4547..6166)
                     /locus_tag="VFQ61_27460"
     CDS             complement(4547..6166)
                     /locus_tag="VFQ61_27460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958275.1"
                     /translation="MIAERGQLVRSLLLLAVASTAWACAANGDDDDAAFSDSPRPGAG
                     SGGAGNAGGVGTGNAGSTGTAVPEPEREAETQYLAPVVSGRFLWTANPLSGRIAIIDA
                     ENFVVQLDTAGDGPTRVLGLAERAGVAGALILNERSDDATLLRILPDGSREKLPPFRT
                     QGDANAWAGSPSSEWAIAYTQVRAEDSISNLLTYQDITLFRLRRGAEKAYALSVGPRP
                     KSFVFDAQEQHAYAVTQAGISVIDLAGVPRVSGLIEISDDPFANPADLDVGFSRDGSR
                     AVVRRSGATNISVLALPSGARRDIELGSTITDLDLSENGELAYAVLGPEHAVVTVPLS
                     APTESSRWPRTELPEESFGALRLNADESAAVVYSTVLDSTRATVLDVSPRGELSAPRS
                     FELISRVSAMFASPDPRFALSFQQPAPGSTRRGVFSLISLAAERTPKIVGTGTPPLEV
                     AFDPEGTAALLSVGDFQQRAYGAFLIDLAAQRVDGLELASPPLAVGVVPAAHRGYVAQ
                     AHPEGRVTFIALDGSDVRTLTGFELAARIRE"
     gene            complement(6156..>6797)
                     /locus_tag="VFQ61_27465"
     CDS             complement(6156..>6797)
                     /locus_tag="VFQ61_27465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958276.1"
                     /translation="EVGLLDLRADLSTPRAVTRVEVSDFLGSVSVSPTGERAVLYTTA
                     TELPRVTILDTSSADSFGAALRTENVRAPVRAAFVAPDDRTAIVALRDQRGNTVTEAF
                     ALLPLDEALPAKIQVTEAEVTGVAFSPPPTTSAVLISASAVTSYVAHFPSLQVDGVKL
                     SHLPLAAGVVPEERLAYVLQSHPDGQLALIDLETAELRNLTGFELAGGVRDDR"
BASE COUNT         1299 a         2189 c         2175 g         1134 t
ORIGIN      
        1 gctcgaggac gtgagtcgca aggcgtaggc atgccgctcc ttaacgaaca tttgccgcga
       61 aaacccgact gcgctgaaac gaacactcac actccagagt gccgcgcgcg agcctgtatt
      121 cggcggcgtg ctcagactgc gaattcgtgt tcgagatccg acgctcagag tcgctcacta
      181 tggctcacaa gaatccgttc acgagctcga gcacggaaaa aggcgtgagc cccattatgt
      241 gccgctcagc cggtgccgca caccgttcgg aaagttcgaa gcgcgagtcg caccgaacga
      301 gcgaaggaga atgggaatgc tttcaggagg aacctatccg cgcctgctga tcctcgcctc
      361 ttggatcagc gtgtttgcgc tggcgggagg ttgtggtggc ggcggcgacg acgcgggccc
      421 gagtggcggc acggacaccg agaccggagg caccggtgat tcgccgggca gcggtggcaa
      481 cgacgcgagc ggagggcgaa gctcttcgaa cggtggaacc aagcccggtg gtgaaaccgg
      541 tggcaccacg gccgaaggtg gtgcagcgcc gggaccggaa ggtggcgctc cgcccgttac
      601 ggtcagccca gatcccgatt tcgaaggcat cgacccgacc gaggttccgc tggaggccac
      661 ccccggctgc gaaggcggct tcaaccccga atcaggcgcg ttgagcctcg acgtcgagga
      721 gacatcacac tttctgctgg tcgacgcgtt cgaagggaac gtccgcgcaa acggtcagat
      781 ctgcagcgca gaagacggca cccccgcttt ggcggaggca ctgaagaaat taaccatcgt
      841 cgggagcgac gacgcggatc gcgtgattct ggacatgctg gatggagcct tcaacccgga
      901 gctattggcg ggggaagatg ctctgcacgt ggatctggga gcgggcacgg attctttctc
      961 gatccgcgcc acgcgagaag atgaccaagt cacgtgtgac ggcgggccct cgccgactcg
     1021 aaatctggtg actttcggca aagccgacgc ggctgcgctc actctcgatc acgccgaatc
     1081 gctgctcgtc agcctcgggc ccggcaacga cgccttcctg ggctatgcta gcgaccaccc
     1141 ctgcacatta cacattgtcg cgttcggcgg tgacgggatg gacttgctgc aaggtgggac
     1201 tcaaaacgac gtgctgaacg gtggtgaggg cagcgacgag ttcgagatgg ggcccttccg
     1261 cgatggcgcg gatctgatca acggcggaga cggggacgat ctcgtctcgt atgctcaacg
     1321 ccgctcagac ctacgtctca agctttgctc ggcgcccgac ctgacgggtt gcgcggagga
     1381 gacgtgcaag tgtgaagctt tgaacggaga gacggcagaa gccgatcgcc tcgtcaacat
     1441 cgaagacgca gacggcggag acggcgatga catcatcatc ggcgacgaaa acccgaacgt
     1501 gctggtgggt cgcggcggcg ccgatcgcat cgacggcatg ggcggcaccg atcaaattta
     1561 cggcgaattg ggcgacgaca cgatcaacgg cggcgccggt gatgacatct tgatcggcgc
     1621 tcagggcaag aatttcgtgg acggcgcgct cggtgacgac atctgcttct tcgctccggg
     1681 cgacaagcct ctcgccaact gcgagacgcg catcgactcg ctctgacggc tcgagctcgt
     1741 ggccaaaaca tggcctcagc ttgattcaca caccccgact cggatgagag tcggggtgtg
     1801 tgcattttgg cgattcagaa gctcaaggcg gcggcgagcc ggtagtacgg gctggtcagc
     1861 gcgagctccg gcaactcgat gtctcgggag cgagcgggga cgcccgagtc attcgtcaac
     1921 gcctgcgaaa gcgccgggaa gtagagcaat ccaccctccg cgatcaacca taggcgggca
     1981 gtccgcttgt gcgcggatcg gctgctcgcc acggcgtagg cgaagccagc cccgatttcg
     2041 cccgtgaagg tcagatgctt gcgttcgaac tgttgctcgg tgacccgatc atcgagcgtg
     2101 ctcgccaccc gcgacaaccc cattccgatg tggccgaagg cgtagccctg acgcaatacg
     2161 tggtaacgca gctccggcaa gagttcgaag cgggtgaccc gcaaggaagt atccgcgccg
     2221 cgcgccgtgt cattgcgggc gccgtggtcg atgccgaacg cgcccaccaa cgatagcggt
     2281 cccgagacga agacggtatg cccggcgttg aagctgacat aacccagcac gtcgctgctc
     2341 gcgtacggat cgaagccgct actcttcacg agcgaactct tgaagcccac gctgacgaag
     2401 ccatgcttct gaaaacgacc cagtgaacca tccgtcggct cctcgggttg gtcgacgtcg
     2461 agttcgcccg ctgactgcac tacatgcgtc gggttcgctc cagcggacgc cgcaggcctc
     2521 gccgccggag gactagcggg cgcggtcaag gttggcgcgg gcacgatcgt gctgttcggc
     2581 ccagctaccg gagcagatgc tgccggagca gacgctgccg cgctattggg agcaccagct
     2641 gggttctgcg cggccggcgc cgccggagag gagggcgcgt cagcgccgct ggttgcggga
     2701 accgccgatt cgttcgggag gttggatttc gacgcttcgc ccagtcgcgt ttgcgcgctg
     2761 acgacgtggc tccatcctgc gctggccatc aaggccgtcg cgatcaccaa aactctcgac
     2821 attcagatac cctccaaaat caagccgctc ttgaaggtcg tttgcgcgga atcgggtcga
     2881 tcggcgtcga agagtgtaac gccccaatct ccgctgccgc cgtgcaacac cagcgcgctt
     2941 ttccccccgt ctgcgcgatt cacttcgaac acgcgcgtga ccggcaactc cacatcgtaa
     3001 gaagtcggat gtttgtccga caggttcagg cgcgcgaagc gagggctgta ctgcggatag
     3061 gcccagactc gttcgccgtc cggcgagaac gagacgttga atccctgcag ggcttgcatc
     3121 gcgtaactca gtcggctctt caaatccagc agagagaagc tcacaccgtc actcgacagc
     3181 agcagcttca tgtacccgta ttcgttgccc ggcacattca tgatgctcgc gaccggcacg
     3241 gaggtgccgt acaaatcgaa gcttgcgtat ggagccgcgc ccgcgtcacc gagccgccag
     3301 aaggcaatgc tcgaagtgcc aggaccgtag agtagcgcga cgtcggcagt gccttcgtcc
     3361 tcaccggaaa ggcccgtgat gcgagccatg cccgcgaact tcgccccgaa ttggaccttc
     3421 tcgctgcgcg aagtcgtcgg gtcgatcaac acggcctggc ttcgattcgg cacgagcacc
     3481 gccagccgca gtccgtggtc ggtctgcacg aagtcgatgg tggaggccac atcacccgca
     3541 tcgagcaagt tggggcgcaa cgagaatgca tgcgcgctcg tagcaccttc ggctagctcg
     3601 agcgtcatca cgctcgcgtc gttggcgaag cgcacggcca ggtatgccgg cgtctcctcg
     3661 ctcgcgtcca cgtagaccac ctgagccgga tgggcgggct taccttggct cgcaccgggc
     3721 aacgtcgtgc tcgggactgg ggccgtcacc tccggtgctt cgggagcctc gaggtccatc
     3781 acggccacgt cttgatcggt ctccaccacc aggaatcgat gcgggcccga gcccggaacc
     3841 tggagcgcgg tcgtgaaggt gaactgctcg ggtcgaccgc cggtgctccg aatcgttttg
     3901 ggaatgggtg cgcgatcggg gtgggccaga tctacgagaa cgaactcgtt gacgttcacg
     3961 accacgccac cggaatcgta gacgacggcc caatcacctt ggggatccaa ggtgatcttc
     4021 tgagcgggat cctccagcga ataggtctgt tcgagcgcgg gcgcgacgcc cgtcttgaac
     4081 acgctgagcc gcggcttctc gtcggcctca ttgactcgac gctgctgccc gcgcgtgagc
     4141 acgtatagat gctccaaatc ccgtgagggc acggccgcga cgatattttg ccctaccggc
     4201 aagcgcgtca cgttcaattc gccttggccc ggagaggtca gcatcaagag ctcattgcgc
     4261 ggtagatccg gaatcgcgac cgcccctcgc aatccgatcg gttcaccgga tgttgccggc
     4321 agatcccaaa acgctttgcg ctctccgcaa cccagcaagg cgaggcaagc ccacgccatg
     4381 ccaccgaatg cccagaacct acgcccggga gcgcggagct cggtcaccga ggagactcgc
     4441 ctcggatggg gtgggtttcc agcgatcgaa cgcggcgcgt tcggtctggc caagagccat
     4501 gattgagaac acatgctcgc ttaactccta tgattgaaga aagacgtcac tcgcggatgc
     4561 gcgccgccaa ctcgaagcca gtcaaggtcc gcacgtcgct gccgtcgagc gcgatgaagg
     4621 tcacgcggcc ctcggggtga gcttgcgcga cgtagccgcg atgcgcggcc ggcaccacgc
     4681 ccactgccaa cggcgggctg gcgagttcca aaccatcgac ccgctgtgcc gcaaggtcga
     4741 tcagaaacgc gccgtacgca cgctgctgga agtctcccac gctgagcaat gccgcggtgc
     4801 cttctggatc gaacgccacc tcgagcggcg gcgtgccggt gcccacgatc ttgggagtgc
     4861 gctcggccgc caacgagatc aagctgaaca ccccgcgacg tgtgctacca ggcgccggct
     4921 gttggaagct cagcgcgaaa cgtgggtcgg gcgaggcgaa catcgcgctc acgcgcgaaa
     4981 tcaactcgaa gctacgcggg gccgacagct cgccgcgagg agaaacgtcg agcaccgtgg
     5041 cccgggtgga atcgagcacc gtggagtaaa ccaccgccgc ggactcatcg gcgttgagtc
     5101 gaagcgcgcc gaacgattcc tccggcaatt ccgtgcgcgg ccaacggcta gattcggtcg
     5161 gggccgagag cggcaccgtc accaccgcgt gctcgggccc gagcacggca tacgcgagtt
     5221 ccccgttttc cgacaagtcc agatcggtga tggtgctccc caactcgatg tcacgccggg
     5281 cgcccgaagg cagcgccagc acgctgatgt tcgtggcacc cgaccggcgc actacggcac
     5341 gcgagccgtc tctgctgaaa cccacgtcga gatcggccgg gttcgcgaag gggtcatccg
     5401 agatctcgat caagccgctc acacgcggaa cacccgccaa gtcgatcacg ctaatcccgg
     5461 cctgggtgac ggcgtaagca tgctgctctt gcgcgtcgaa gacgaaagac ttgggtcgcg
     5521 gcccaacgct caaagcatag gccttttctg ccccccgccg caggcggaac agagtgatgt
     5581 cctggtacgt gagcaaattc gagatcgagt cttcagctcg gacctgtgta tatgcgatcg
     5641 cccactcgga agaggggctg cccgcccatg cgttcgcgtc cccctgggtt cgaaacggcg
     5701 gcagcttttc ccgagatccg tccggcaaga tgcgcaacaa cgtcgcgtcg tcggagcgtt
     5761 cattcaagat caaggctccc gccacgcccg cacgctcggc cagcccgagc actcgagttg
     5821 gcccatcgcc ggcggtgtcg agctgcacga cgaagttttc ggcgtcgatg atcgcgatcc
     5881 gcccggagag tggattggcc gtccacaaga agcgcccgct caccactggc gccagatatt
     5941 gagtctcggc ctcgcgctcg ggctccggca ccgccgtgcc ggtcgatcca gcgttgcccg
     6001 taccgacccc gccggcattg cccgcgccgc cgctccccgc gccggggcgc ggggagtcac
     6061 tgaatgcggc gtcgtcatcg tcgccatttg ctgcgcaagc ccaggccgtg ctcgcgaccg
     6121 cgagcaatag cagcgaacgc accagctgtc cgcgttcagc gatcatcacg cactcctccc
     6181 gctagctcga agcccgtcag attgcggagc tccgccgtct ccagatcaat caacgccagt
     6241 tgcccgtcag ggtgcgattg caacacgtaa gccagcctct cctcgggcac gacgcctgcc
     6301 gccagcggca gatgagagag cttcacgccg tctacctgta gggacgggaa atgggcgacg
     6361 tagctggtaa cggccgaagc ggaaatcagc accgcgctcg tggtgggcgg tggagagaac
     6421 gcgactcccg tcacttcggc ttccgtaacc tgtattttcg cgggcagtgc ttcatcgagc
     6481 ggcagcaagg cgaacgcttc ggtcaccgtg ttgccacgct gatcacgcag agccacgatc
     6541 gcggtgcgat catccggcgc cacgaaggcc gcgcgcacgg gtgctctcac gttttcggta
     6601 cggagcgcgg caccaaagga atcggccgag ctcgtgtcga gaatcgtaac ccggggcagc
     6661 tccgtggccg tggtgtagag cacggcgcgt tctccagtcg gtgagacgga gacgctgccc
     6721 aagaaatcgg agacctcgac tcgggtaacc gcgcgcggcg tgctcaggtc cgcgcgcaaa
     6781 tcgagcaacc cgacttc
//
LOCUS       DASTKU010000785        12486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1046917, whole genome shotgun sequence.
ACCESSION   DASTKU010000785 DASTKU010000000
VERSION     DASTKU010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12486
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046917"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VFQ61_27470"
     CDS             <1..614
                     /locus_tag="VFQ61_27470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HET9958277.1"
                     /translation="FVVGSAETLAYAVATGTLGDPRSFKRPVRVTVPRTLPTDDVLIV
                     RKAKAKTKEGEAVKGTPAEQVATAGWKAAQVLPVVANRKVPNEPSAMVLETLDDVRWV
                     VRRAPDIHPLVRAVVAPFIPSGCVPVLAGVGILALSAAPETIEQLKAAGSLSVAEPAE
                     WNGNGSPIKDANQAPITLKWLAVGAERQWTELGTAWVPAPAKR"
     gene            739..1365
                     /locus_tag="VFQ61_27475"
     CDS             739..1365
                     /locus_tag="VFQ61_27475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958278.1"
                     /translation="MRRWLKLLSVSAFVAALTGAASEAHALDYGHGGQFGLRAALTAG
                     YRMVLRYDESPYCRDYDVTKPASDQAQFCGFPAPLALDLGLSFAPLDFVEPYLWARFG
                     LAREKETNSKPLTLVGAGARLYTMSGSPLKIFVEPAVGLELEGGNGDPRFVNKTYRND
                     LVFHLAAGPQIDLASNVGLYADAGLNLGVLRALQSSLDLRIGVQGRFP"
     gene            1362..1844
                     /locus_tag="VFQ61_27480"
     CDS             1362..1844
                     /locus_tag="VFQ61_27480"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9958279.1"
                     /translation="MTNRPFRVLGVQQIAIGHADKKRLKALWSDLLGIEIKGHFRSES
                     ENVDEDIGVLGAGALAVELDLMQPIDPTRSPRVHEPALNHLGLWVDDLSAAWEWLSAR
                     GVRFAGGIRVGAAGHRVAFIHPKGNAESPQSGEGVLIELVEAPVEVQNALRTLSQFGA
                     "
     gene            complement(1907..2212)
                     /locus_tag="VFQ61_27485"
     CDS             complement(1907..2212)
                     /locus_tag="VFQ61_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET9958280.1"
                     /translation="MSRNARKGLLLLQRVVPLAVLGLAAISVPVMVFSPEGLARLEHL
                     KREKQRADEEVSRLSESIRALRAEVAKVKDDPSMVERTARDELGLLRKTEVVFLFKD"
     gene            2378..7351
                     /locus_tag="VFQ61_27490"
     CDS             2378..7351
                     /locus_tag="VFQ61_27490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9958281.1"
                     /translation="MPTQTAIDLIQAEFERRFELAELRDLARDMLGPQAATLARASSK
                     RDLIGQLLGALQKQGGMLALRELLGARLPGLAKDFPHGQEDARAQRLPVEFERALELS
                     SSERLGAGRRGHCYLVARQSQRFRLKVYYPQVTRERERQLAFAALTRHLARCAPPQWP
                     ERVEYWELAGISGVCHAYPEGALASARLATAWSEASPFEPKELAPWLRSALRALEVLH
                     QRGLAHGNLSPSNIAWHAESCTLLDAFEDRLASGPLEEELPGWSRLSPERLLGGRSDA
                     SSDLFTLGAVALELLVGAPAVGAWQALQFALESPARASRVDTLSPAALQRLPAGWVRS
                     FLRELTTRDPKLRPARAEAALARLEALISSEPSAPSLLSAVSGPFALPFVDSHTPLQD
                     VPEALGGTPTSQRSIASASRALGSDSLRAPVAPQGAVSLQSTSDADSKSAGSHGKAEH
                     SKADSQSTATQQLAAEVASKPAPSKPDSTEPEAITNINAAALKSEVTVHAGEILDAAD
                     AEAELERLLALTEQSQDRRERARFLGRIGQLYEARQSEPEQALFAYTQALCENPFENA
                     WLSRISALVGERESAWLEVLSSVETALADASRSHEERAVLARRGADLGRAHVPHADLT
                     LRLADAARALDPEENSVAAQLEKQGAFQDLLEHLKQQIERADTPRKRVTLQLRLAELY
                     EVEFLDGPRAAAILEQVLEVFPAHEEAATRLAGAYRRLARFHDLVALCERRLQSEPRS
                     EAKLELCLESARVLEGPLGDPERAERAYQAALELAPECVPALEGLSRLAEARQDPAAA
                     RYLQSLATHAASSEARAQSWVRAARSFEHWGKVNDAIESYKRALDADANCRPALRGLV
                     ETLKSSGDLTGAASILERDLEHGSAASPRDRSASLAELSLMYRARGENERARRNAKAA
                     IELDGTNYVAARALADLEFDEQHHSEAARWYSVLLSAAEKPSDAGAVALRHIEALLRA
                     GQVSKAIEVAPRWLALAPGDLQLWHRAAGLEFEHGSKERAVEQYDELLRRFSSDLPAT
                     ERTELWVRLGQALESLGRYTDAVEPLERALRQDPQQRVALGALGRVVEKLEQWLRAID
                     LKLRLVALASGEERVQLLLEVAELWASKLGERNEAISALNRALESGTEDRRVLTKLMQ
                     LHGEAEHWPELSEVVLRLLDRVDEPQQRVKYLHTVAIVTARHLGDIPKALTYYARAIE
                     LAPDFERGILESLELQRKLGDWVAVEQLLRRKLDLLTSRGDQAGMVRTFDELAELYQY
                     RLLWPEQAVDAYEAAQTLDFANRARSEVLERLYMTAPAKYLERAVASAHEILRKDPFR
                     SEPYRMLRRLYVETGQADAAYCVCQALTVLNLASAEEKQLYESNRSRTAVPALHVLSD
                     DAWLEHVMHADADPLLTSLFALIEAAVIAKRSRTLAEFGYDPARALSLAEHPSPLCQS
                     LFHAASVLGITPPLVFASASDPSPGDPGSISFLFTAKPAFVLGPAALSPELPLQHAAF
                     VSGQSLAYLRPGMYLRQLLSSGTALKAWFFATIQLTAPAFPVVPEIEGAVREAAIALE
                     AGIVGPLRDHLTRVVAKLVATGAELDLKRWVTALDFTADRAGLIACHDLKAAINAAQA
                     FANSAIVPGRFQAPLEERLRELIIYSVSPDYLEVRRSLGVGSGARSE"
     gene            8651..10492
                     /locus_tag="VFQ61_27495"
     CDS             8651..10492
                     /locus_tag="VFQ61_27495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9958282.1"
                     /translation="MMHSDAFDMQGDGRGAIPERYEVISPLGAGGMARVYLALHRGPF
                     GASKLVVLKQLRAEIASDPQYRNMFADEARLALRFNHPNVVHTYEVVDEPGVFFLAME
                     FLEGRSLSQLLAKITRERMPIDEHLWILTQVLAGLHYAHTLADFDGTPLRVVHRDVSP
                     SNVFVTYTGEVKILDFGIAKVAGAVAETQLGMIKGKIGYAAPEQCVAEPVDARSDVYA
                     VGVMLWEALARRRRSVGDTSMAVIQARLLHAEPNIEDVVADLPPDLVRITRRALAVNP
                     AQRFSSALEMQLALEAYLRRRGFITFRERVTKLMVTHFTDELVALRQLIGDRVGSRGR
                     VSRSSIPDRPGAASEVSTQSITPPGASGSVMQIAQAARQARRRWLAYGITGILATTLG
                     GLGARYFAVGSSNTAANQRAVSTQVVPTRISASAPAERATPSPDSNPLAAAGSASASG
                     APTSSAGTPADTANSTRKVQLSITVTPKHALVRVDGKLVSTPFHAPFDVTGEKHTISA
                     DAAGFIAEKRVLVLDREVNLNFQLRPSPAEGVPESAPSSGRTASPVASADEQPSPAPA
                     EVVTSVSPPPSPAPPARLEPGEDLRRSPSESQSRKSRGIDPNDPYGL"
     gene            10645..11697
                     /locus_tag="VFQ61_27500"
     CDS             10645..11697
                     /locus_tag="VFQ61_27500"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9958283.1"
                     /translation="MKYNCIAACAVCLNLYSAVALAQPAAASRTALPAPQAVLAQPAS
                     ATQPSTPEVSNAQVEEARAHFQRGVELYQERNFEAALAEFSRAHELAANYRVLFNMGQ
                     VHVERHDSVAALKAFRRYLDEGGADVPAARREQVERDIVMLKQRIAELRVRCNVNDAE
                     ILVGDTAVGKTPLTEPVLVNAGVIHLTLRKTGYAPSSRTLTAVGAEKQELEFVLEPEQ
                     GNAGTANAAPAGTALPVIEERRNMTPFWIGLGTTVALAGGATVFGVLTGRADNRLDDA
                     LRTYPTKESEIEDARSELRRNALLTDVLAGGALVSAAVTIYFAVSPPTVHEARTARVG
                     LRPYAGGTGLELSGNF"
     gene            11702..>12486
                     /locus_tag="VFQ61_27505"
     CDS             11702..>12486
                     /locus_tag="VFQ61_27505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958284.1"
                     /translation="MPRTLTFSLVLGLPFLTACSLSIDADRVQCKETADCRALGQAFS
                     DTVCINQICRADPKWACLDDPEPIVSEGTGPFEVKLPLIVLNAPAPNNKLPGASVKVC
                     TRLDGDCLNPLDTLESDEDGILNLTLNANFSGYLEVQKQGFTTTLYFFNPPITRSQTL
                     ANLSLSPTQLREGIVGLLQASPERGDILVQALDCTGKQAPGVRLELGEGKTSQAEAIQ
                     YYLEDNTPARGRDETDVTGYGGFLNVNPGQVVLNAKVAENGRQ"
BASE COUNT         2361 a         3927 c         3967 g         2231 t
ORIGIN      
        1 agttcgtggt cggctccgcc gagacactcg cctatgcggt cgcgacgggc acgctcggag
       61 atccccgatc cttcaagcgc ccggtgcgtg tcacggttcc acgcacgtta ccgaccgacg
      121 atgtactgat cgtacgtaag gcaaaagcga agaccaagga gggtgaagcc gtcaaaggca
      181 cgcccgccga gcaggttgcc acggcgggtt ggaaagccgc gcaggtgctg ccggtggtgg
      241 ccaaccgaaa agtgccgaac gaacctagcg cgatggtgtt ggagactctg gacgacgtgc
      301 gctgggtcgt tcggcgcgcg ccagacattc atccgctcgt acgtgccgtg gtggctccct
      361 tcatcccgag tggctgcgtg ccggtgctcg ctggggttgg catcctcgcg ctcagcgcgg
      421 cgcccgaaac gatcgagcag ctcaaagcgg ctgggtcgtt gagcgtagcc gagccagcgg
      481 agtggaacgg caacgggtcc cccatcaaag atgcgaacca agctccaatc actttgaaat
      541 ggttggccgt tggcgccgaa cgccagtgga ccgagctcgg tacggcctgg gtgcccgcgc
      601 ccgccaaacg ctaggaagcg acgcgcggga accgcggcag caggaccgca ccaaagcagg
      661 accggcgcga ccacggcgtc atgcccttca agccaagggg tttccggaga agcgggatcg
      721 gtttatcgtt cccgcctcat gcgcaggtgg cttaagcttc tctcggtctc cgccttcgtc
      781 gcagcgctca cgggcgcagc ctccgaggcg cacgctttgg actatgggca cggaggccaa
      841 ttcggtctgc gtgcggccct gacggcaggc tatcgcatgg tgctgcgcta cgacgagtct
      901 ccgtattgca gggactacga cgtgaccaag cccgccagcg atcaggctca attctgcggc
      961 tttcccgcgc cgctcgcgtt ggatctcggc ctaagctttg cgccattgga tttcgtcgag
     1021 ccgtacctgt gggcccggtt cgggctcgcg cgcgagaaag aaaccaacag caagccactc
     1081 acgctggtgg gtgccggcgc gcggctctac acgatgagcg gctcgccttt gaagatcttc
     1141 gtggaaccag ccgtcggttt ggagctggaa ggcggcaatg gcgatcctcg cttcgtcaac
     1201 aagacgtatc gcaatgacct cgtgttccac ttggcggcgg gccctcaaat cgatctcgca
     1261 tccaatgtcg gcctctatgc agacgcgggc ctgaatctcg gagtgctgcg agcgctacaa
     1321 agttcgctcg acttgcggat tggagtacaa ggacggttcc cgtgacgaat cgccccttcc
     1381 gagtgcttgg agtccaacaa attgccatcg gtcacgctga taagaagcgg ctgaaggcac
     1441 tttggagtga ccttttgggt atcgaaatca aagggcactt tcgcagcgag tccgagaacg
     1501 tggatgagga catcggtgtg ctcggcgcgg gcgcgttggc cgtggaactg gatcttatgc
     1561 agcccatcga tccgacgcgc tcgccacgtg tccacgaacc cgccttgaat cacctcgggt
     1621 tgtgggtcga tgatctgagc gccgcttggg agtggctcag cgcgcgcggg gtgcgcttcg
     1681 cggggggcat acgagtcgga gctgcgggcc atcgagtggc gttcattcat cccaagggca
     1741 acgcagaatc gccccaaagt ggagagggcg tactcataga gctcgtagaa gccccggttg
     1801 aagtccaaaa cgcgctacgg acactgagtc aattcggcgc gtagaacacc gcgccagagc
     1861 acggcgaatc taccgctcgg gcagtccaac caagcgagcg agtcgctcaa tctttgaata
     1921 ggaaaacgac ctcggttttg cgtagcaaac ctagttcgtc gcgcgcggta cgttctacca
     1981 tgctcgggtc atccttgacc ttggccacct ccgcccggag cgcgcggatg ctctcggaca
     2041 agcgagacac ttcctcatcg gcgcgctgtt tctcgcgctt caaatgctcg agacgagcca
     2101 ggccctccgg tgagaacacc atcaccggca ccgagatcgc ggcgagcccg agcacagcga
     2161 gcgggacgac gcgctgtaga agcagcaaac cctttcgcgc gttgcgactc atctcgtgtc
     2221 cttcatcggc atgtttcgct cattggcacg atcctcgata ggatcgccgt aggcagcttc
     2281 tgctgacctt aggcagcagg ccaagtcttc tgcaaaaaaa actccgcggg tgggtacgag
     2341 cctgtatggt tcacactcgc gcttggcgtg ttctcctatg ccgactcaga cagccataga
     2401 tctcattcaa gccgagttcg agcgtcgctt cgagctcgcc gagctgcgcg atctcgcgcg
     2461 ggatatgctc ggcccgcaag cagcgaccct ggcgcgcgcc tcgagcaaac gcgatctcat
     2521 cggtcagctg ctcggggctc tgcagaagca gggcggaatg ctggcgcttc gtgagctgct
     2581 tggcgcgcgc ttaccggggc tcgcgaagga ttttcctcat ggccaggagg acgcccgagc
     2641 tcaacggctg ccggtggaat tcgagcgcgc actcgagctc agctcatcgg aacgcctcgg
     2701 cgctgggcga cgagggcact gctacctggt cgcgcgccag tcgcagaggt tcaggttgaa
     2761 ggtgtactat ccgcaggtca cgcgtgagcg cgaacgccag ctcgcgttcg ccgcgcttac
     2821 ccgccacctc gcgcgctgcg cgccgcccca atggccagag cgcgtggaat attgggagct
     2881 cgcggggatc tctggcgtgt gccatgccta tcccgagggt gccttggcga gtgcgcgact
     2941 ggcgacggca tggtccgaag cgtccccctt cgaaccgaaa gagctggccc cctggctacg
     3001 ctcggccctt cgcgctctcg aggttctgca ccaacgcggg ctcgcgcacg gaaacttgag
     3061 tcctagcaac attgcttggc acgcagagag ctgcacactg ctggacgcct tcgaggatcg
     3121 acttgcctcc ggcccgctcg aggaggagct cccgggctgg tcgcggcttt caccagaacg
     3181 cctgcttggc gggcgctcgg acgccagcag tgatttgttc accctcggcg cggtggcgct
     3241 ggagttgctg gtcggtgcgc ccgcggtggg tgcttggcag gcgttgcaat tcgcgttgga
     3301 gagcccggcc cgtgcctcac gggtggatac cttgtcgccc gccgccctgc agcgcttgcc
     3361 cgcgggctgg gtgcgcagct tcctgcgcga actgacgaca cgggacccaa agcttcggcc
     3421 ggctcgagcc gaggcagcgc tcgctcggtt agaagcattg atcagcagtg aaccgagcgc
     3481 gccgtcgctg ctgagcgcgg tttcggggcc cttcgctctg ccgttcgtcg actcgcatac
     3541 gccactccag gatgtgccgg aagcgctcgg tggtacaccg acgtcgcagc gctcgatcgc
     3601 gtcggcttcc agagcactcg gctccgactc gctgcgtgca ccagtagccc cccaaggcgc
     3661 tgtcagtctg cagagcacga gcgatgcaga cagcaagtcc gccggctcgc acggcaaggc
     3721 cgagcatagc aaggccgact cgcagagtac ggcaacgcaa cagctcgcgg ccgaggtcgc
     3781 ctccaagccc gccccaagca agccggatag taccgagccg gaagcgatta cgaatatcaa
     3841 cgcggccgcg ctgaagtccg aggtcaccgt gcacgcgggg gagatcctcg atgccgctga
     3901 cgcggaggcc gagctggagc gcttgctcgc gctcaccgag caaagccagg atcggcgtga
     3961 gcgcgcacgc ttcctcgggc gaatcgggca actttacgaa gcgcgtcaga gcgagcccga
     4021 gcaggccttg ttcgcgtata cccaggcgct ctgcgaaaac ccattcgaaa atgcgtggct
     4081 ctcgcgtatt tcggcgctcg tcggtgaacg cgagagcgct tggttggagg tgctgtcgag
     4141 cgtcgaaact gcgctcgcgg acgcatcgcg gtcgcatgaa gagcgtgccg tgttggcgcg
     4201 tcggggcgcc gatctgggcc gcgcgcacgt gcctcacgcc gatctgacgt tgcgcctggc
     4261 cgatgcggcg cgcgcgctcg atcccgaaga gaactcggtg gcggcgcagc tcgaaaaaca
     4321 aggagctttt caggatcttc tggagcacct gaaacaacaa atcgagcgcg cggatacgcc
     4381 tagaaaacga gtgaccctgc agctacgtct ggccgagctc tacgaagtcg agttcttgga
     4441 cggccctcgc gccgccgcca tcctcgagca ggtgctcgag gtattcccgg cccacgaaga
     4501 agcggccact cgcttggcgg gcgcctatcg tcgactggct cgctttcacg acctggtggc
     4561 gctttgtgag cggcgcctgc agagtgagcc gcgctcagaa gcgaagctcg agctatgcct
     4621 ggaaagcgcg cgggtgctcg aagggccact cggggatccc gagcgcgccg agcgcgccta
     4681 tcaagccgca ctcgagctcg cgcccgaatg cgtgccggcg ctggaaggtc tttcgcgact
     4741 ggccgaggcc cggcaagatc ctgccgccgc ccggtatctc cagtcgttgg cgacgcatgc
     4801 ggccagctcg gaagctcgtg cgcagagctg ggtgcgtgcg gcgcgatcgt tcgagcattg
     4861 gggaaaggtc aatgacgcca tcgagagcta caagcgagcg ctcgacgcag acgccaactg
     4921 tcggccggca ctgcgcggct tggtcgagac gctcaagagc tcaggcgact tgaccggcgc
     4981 ggcgagcatt ttggaacgcg atctggagca cggctccgcc gcgagcccgc gggatcgctc
     5041 cgcgtcactt gccgagttga gcctgatgta tcgcgcgcgc ggcgaaaacg agcgcgctcg
     5101 ccggaacgcc aaggccgcca tcgaactgga tggcaccaac tatgtcgcgg cgcgagccct
     5161 ggccgatctc gagttcgacg agcaacacca ttcggaagcc gcccggtggt actccgtgct
     5221 gctgagcgcg gcggaaaaac ccagcgacgc cggtgcggtc gcgctacgcc atatcgaggc
     5281 cctgctgcga gcaggccagg tgtcgaaggc gatcgaggtg gcgcctcgtt ggctcgcgtt
     5341 ggcccccgga gaccttcagc tctggcatcg cgccgccggt ctggagttcg aacacggcag
     5401 caaagagcgc gccgtggagc aatatgatga actgctgcgc cggttctcca gcgacttgcc
     5461 agcgacggag cgcaccgagc tgtgggtacg cctgggccag gcgctcgaga gcttgggtcg
     5521 atacactgac gcagtcgagc cgctcgaacg tgcactacgc caagatccac agcaacgcgt
     5581 ggcgctcggg gcgctcgggc gtgtcgtcga gaagctggaa caatggctac gagccatcga
     5641 cttgaagctg cggctcgtag cgctcgcctc gggcgaggaa cgtgtgcagc tgttactcga
     5701 ggtggcggag ctctgggcga gcaagctcgg tgagcgcaat gaagccattt cggcgctcaa
     5761 tcgcgcgttg gagagcggca ccgaagatcg gcgcgtgttg accaagctca tgcagttgca
     5821 tggcgaggcc gagcactggc cggagctatc ggaggtcgtg ctgcgcttgc tggatcgggt
     5881 ggacgagccg cagcaacgag tgaagtacct gcataccgtc gcgatcgtga cggcgcgcca
     5941 cctcggcgac atacccaaag ccttgacgta ttacgcgcga gcgatcgagc tcgcgcctga
     6001 cttcgagcgc ggcatcctcg agtcgctcga gcttcagcgc aagttgggcg actgggtggc
     6061 cgtcgagcag ctgctccgca ggaagctcga cctgctgacc tcgcgcggcg accaagccgg
     6121 catggtccgc acattcgacg agctcgccga gctctatcaa tatcggctgc tttggccgga
     6181 gcaagcggtc gacgcctacg aggccgcgca aacgctcgat ttcgccaacc gcgcgcgcag
     6241 cgaggtgctc gagcgactgt atatgaccgc ccccgccaag tacctcgagc gcgcggtcgc
     6301 atcggcccac gaaatcttgc gtaaggatcc attcagatcc gagccgtatc gaatgctgcg
     6361 gcggctttac gtagagacag ggcaagcgga cgcggcgtat tgcgtgtgtc aggcgctcac
     6421 ggtgctgaac ctcgcgagcg cagaagaaaa gcagctctac gagtcgaacc gttcgcggac
     6481 agccgtcccc gcgctgcacg tgttgagcga cgatgcttgg ctcgagcacg tgatgcacgc
     6541 agacgcggat cctttactca cgagcttgtt cgcgctgatc gaagccgcgg tgatcgccaa
     6601 gcgcagccgc acgctcgcgg agttcggtta cgaccccgca cgcgcgctgt ccctggctga
     6661 acatccgtca ccgctctgtc agagcctctt tcatgcggca tcggtgttgg gcatcacccc
     6721 cccattggtg ttcgccagcg cgtctgatcc gagcccgggg gatccgggaa gcatttcgtt
     6781 cctgttcacg gccaaacctg cgttcgtttt gggcccggcc gcgctctcgc ccgagctccc
     6841 cctgcagcat gcggcttttg tttcgggaca gagtctcgcg tacttgagac ctggcatgta
     6901 cctccgacaa ctcttgtctt ctggtactgc gctcaaagcc tggttcttcg ccacgattca
     6961 gctcactgcg cccgcatttc ccgtcgtgcc ggaaatcgaa ggggctgtac gcgaagccgc
     7021 aattgcgctg gaggccggaa tcgtggggcc acttcgcgat catctcactc gagtggtcgc
     7081 aaaactggtg gccacaggcg ctgaactgga tttgaaacgc tgggtgactg ctttggactt
     7141 caccgcggat cgagcaggcc taattgcttg tcacgatttg aaggccgcaa tcaacgcggc
     7201 tcaggctttt gccaacagcg caatcgtacc cggccgcttc caagctccat tggaggagcg
     7261 gcttcgtgaa ctcattatct attcagtaag tccggattac ctggaagtac gacgttcatt
     7321 gggtgtcggc tccggggccc ggagcgaata actcatctct tcgagtggcg tagttgaaag
     7381 agcggctcaa gcaccggtcg gcgtgagagt ccgctaaggg gtagtgcgaa gagcaggcag
     7441 agctgctgcc gaatcaccga atgaagccga atctgacgaa catgcggcgc cgatcacgga
     7501 tgctcagcta gccaacatcc tagtgtgcaa cgcgcataca tcactcacta gtacgcaaag
     7561 cgcacacacc actcaacgga actcctatgt gcaaaccgcc ttgcaccggc gagcttcgcg
     7621 cgcattccct taaccgcaag gcttctgcgt gatggcagtt gggtagaact tggcttgcgg
     7681 cgtccggagg agcgtcacaa aactttggga ttgtgaaagc aatccgcaaa cctgcaacgc
     7741 catatgcacc tggcaccggt tccggccggg gatcaacatg gcgggggcaa catggccggg
     7801 gatcaacatg gccggggatc aacatggccg gatcaacatg gcggggacaa tttcggcccc
     7861 gaccgagaat accgctgaac ggctacaccg gagaagcgcc ttttccgcca gtccgccccc
     7921 acgggcgctt gtggcgcgcg ccctcaaaag agagcctggc gagcaacctg taacgcgcgc
     7981 agcctcgtac tggcgcggct actcctcaag ccaatcgtta tgcctcagcc actcagccac
     8041 cctctgcggc cagctctacc catccggctc tcccggccat tctcggggca gttctccggg
     8101 cagttctccg ggcagtaggt aggggcgccg cgacccgcca actacccaag attcatagat
     8161 agaacggctc ccgaaaacac tgacttgcca gcacaacgtt tcgagtagta ccgtttcgag
     8221 ttacttagat tgataactgt aacgagcgac tcggcaggca ttcgggcgcg gaatgggaca
     8281 gaggaatggg ccagttggcg tcgcccagcg cgcgcgtcac gtcgtttggt tcggattcta
     8341 cagtagctcg cggtaacttc tggcgacagc gccgaccggg gctaatgttc gaggtccttg
     8401 gtttaggagc ggccagtacc ggcggtggcg tttgcggtag gccggttgat cgtgctgctg
     8461 gactgagtga gctcgggttg gcgagtgaaa cgaatgcttc gcggcgagtt gggttcgagt
     8521 ggccaggcaa gagttcgcgg gagttgatga gaatgacatc acgtgttggc gtagatagag
     8581 cggtctcgcg aggggccgtc gcgccttcgt ggagtcgacg cgcaggggag aagcgccaag
     8641 gcaggggcac atgatgcact ccgatgcgtt cgatatgcag ggcgacgggc gcggagctat
     8701 tccggagcgc tacgaggtca tttctcctct cggcgcgggt gggatggcgc gcgtctacct
     8761 cgctttgcac cgcgggccct tcggggcgag caaactcgtg gtgctcaagc agctgcgagc
     8821 cgagattgct tctgacccgc agtaccgcaa catgttcgcg gacgaagctc ggctcgcgct
     8881 gcgcttcaac caccccaacg tcgtccacac ctacgaagtg gtggacgaac caggggtctt
     8941 ctttcttgcg atggagtttc tcgaggggcg ctcgctctcg cagctactcg cgaagatcac
     9001 ccgcgagcgc atgccgatcg acgagcacct ttggatcctg actcaagtcc tggccggctt
     9061 gcattacgct cacacgttgg ctgacttcga cggaacgcca ctgcgcgtag tccaccgtga
     9121 cgtgagccct tccaacgtgt tcgtcacgta cacgggtgaa gtgaagatct tggacttcgg
     9181 catcgccaag gttgcgggcg ccgtagccga gactcagctc gggatgatca agggcaaaat
     9241 cggctacgcg gcaccggagc aatgcgtggc cgaaccggtc gatgcgcgca gcgatgttta
     9301 cgctgtcgga gtgatgttgt gggaagccct ggcgcgtcgg cgtcgttcgg tcggggacac
     9361 gtcgatggcc gtgattcagg cgcggctact gcacgcagag cccaacatcg aagacgtagt
     9421 cgccgatttg ccgccggatc tggtgcggat cacgcggagg gccctcgcag tcaaccccgc
     9481 acagcgcttc tcgtcggcgc tcgaaatgca gctcgcactc gaggcttact tgaggcggcg
     9541 cggcttcatc accttccgtg aacgagtcac gaagttgatg gtcactcact tcacggacga
     9601 gctggtcgcg ctgcgacaac tgatcggtga ccgcgtcggt tcacgaggtc gggtctcccg
     9661 ctcttcgatc ccagatcgcc ccggtgctgc ttccgaggtg agcacgcagt cgatcacacc
     9721 gcccggcgcg agcggcagcg tgatgcagat cgcgcaagcc gcacgccaag cccgccggcg
     9781 ttggttggcc tacggaatca ccggcattct cgcgacgacg ctcggtgggc tgggcgcgcg
     9841 ctacttcgcc gtcgggagtt cgaacacggc cgccaatcaa cgcgcggtat ccacgcaggt
     9901 cgtgccaacg cgcatcagcg cctccgcgcc tgcggagagg gccaccccaa gccccgattc
     9961 caacccactc gcagccgctg gctcggcatc cgcgtccggc gctccgacga gctcggctgg
    10021 aactcccgca gacaccgcaa acagcacgcg caaagtccag ctgagtatca ccgtcacccc
    10081 gaagcacgca ttggttcgag tggatggcaa gttggtttcc acacctttcc acgcgccctt
    10141 cgatgttacc ggagaaaagc acacgatttc ggcagatgcg gctggtttca tcgccgaaaa
    10201 gcgggttctg gtgctggacc gcgaagtgaa tttgaatttt caattgcgcc cctcacccgc
    10261 cgaaggcgtg ccggagagtg ccccgagcag tggccggacg gcctctcccg tggcgagcgc
    10321 ggatgaacaa ccttcgccag cgccggccga ggtggtaacg tccgtttcgc ccccgccctc
    10381 gccagcacca cccgctcgat tggagccagg agaagacctc cggcggtcac ccagcgagag
    10441 ccagagccgt aaatcacgcg gcatcgaccc aaacgaccca tacggactat gagccctcgc
    10501 ccttagaggc tatagaacct ctgccctagg ggccctaaat aactagggca gcaggatccc
    10561 catccaggcc caaggatcca aaccaatcag aggatccgaa gccgatcaga gatcctgaag
    10621 ccgatcagag gatccgattc aatcatgaag tacaattgca ttgcggcgtg tgccgtctgc
    10681 ctgaacttgt actcggcagt ggcgctcgct caaccggcag cggcctcgcg aactgcgctg
    10741 cccgcaccac aagcagtgtt ggctcagccc gcaagcgcaa ctcagcccag cacgccagaa
    10801 gtgagcaacg cccaggtaga agaagctcga gctcactttc aacgcggcgt ggagctttac
    10861 caagaacgca atttcgaggc ggcgttggcg gagttttcac gcgcccacga actcgccgcc
    10921 aattaccgcg tgctcttcaa tatggggcaa gtgcacgtag agcgacacga ctcggtggcc
    10981 gcgctcaaag ctttccggcg ctacttggat gagggcggag cggacgttcc cgcggcgcgg
    11041 cgcgagcagg tcgagcgcga catcgtgatg ctcaagcaac gtatcgcgga gctccgagtg
    11101 cgatgcaacg tgaacgacgc cgagatcctg gtgggtgata cggcggtcgg aaaaactcca
    11161 cttacggagc cggttctcgt aaatgccggg gtgattcacc tgacgcttcg caaaactgga
    11221 tatgcaccct cgagtcgaac cctgaccgcc gtaggcgccg agaaacaaga gctcgagttc
    11281 gtgctcgagc cggagcaggg caatgcgggc acagccaacg cggcgcccgc cggaacggcc
    11341 ttgcctgtaa tcgaggagcg ccgaaacatg acgccgtttt ggatcgggct cggcaccacc
    11401 gtcgcgctcg ccgggggcgc cacggtgttc ggcgtcctga caggccgcgc agacaaccgc
    11461 ctcgatgacg ctctgcgtac gtatccaacc aaagagagcg agatcgagga tgcgcgcagt
    11521 gagctgcggc gcaacgctct attgacggat gtgttggcgg gcggcgcgct tgtctcggcg
    11581 gcggtaacca tctactttgc cgtctctcct ccgacggttc acgaagcgcg aaccgcgcgc
    11641 gtgggactcc gcccctatgc gggcggaact gggctcgaac tctccggaaa cttctgattc
    11701 catgccgcgc acccttactt tttcattggt cttggggcta ccgttcctca cggcttgctc
    11761 gctatccatc gacgcagatc gagttcagtg caaagagacc gcggattgcc gagcgctggg
    11821 acaagcattc tccgatacgg tgtgcatcaa ccagatttgc cgagccgacc cgaagtgggc
    11881 gtgtctagat gaccccgagc cgatagtttc ggagggtacg gggcctttcg aggtgaaact
    11941 tccgctcatt gttttgaacg cgcccgcacc caataacaaa ctccccggag cgagcgtgaa
    12001 ggtctgcacc cgcctggacg gagactgcct gaatccgctc gatacgctcg aaagcgacga
    12061 agacgggatc ctgaacctga ccctgaatgc gaacttctcc ggctacctcg aagttcaaaa
    12121 acagggattc acgactacgt tatatttctt caacccgcca atcacccgca gccagacgct
    12181 ggccaaccta tcgctttccc ccacgcagct gcgcgaaggc atcgtcggcc tactccaggc
    12241 ctccccggag cgcggggaca ttctggtgca agccttggat tgcacgggca aacaggcgcc
    12301 aggtgtgcgg ctcgagctcg gcgaagggaa gacctcccag gccgaagcga tccagtacta
    12361 cctcgaagac aacacgcctg cgcgcggtcg cgatgagacc gacgtcaccg gctacggtgg
    12421 ttttttgaac gtgaacccgg gacaggtggt gttgaacgcg aaagtcgccg agaatggccg
    12481 tcagtt
//
LOCUS       DASTKU010000786        12976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_823506, whole genome shotgun sequence.
ACCESSION   DASTKU010000786 DASTKU010000000
VERSION     DASTKU010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12976
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823506"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1858
                     /locus_tag="VFQ61_27510"
     CDS             <1..1858
                     /locus_tag="VFQ61_27510"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9958285.1"
                     /translation="RDIEADAVEQASASIVLADDQHVPLRLTASRGTLGLELYAPVEI
                     GPLKITSLLLSLPGLKFPLDLSGGVPKFRHRRGELQEMTLGLSFAALARWLGARSGEL
                     TQGLSRAPALFRAPDGLTVGLVWGRRALAFDLVWAPDRDAARCVITRARGMGLDLPAL
                     GLALRVADTWFGAWAERRGRVIRFPNVPQRVGRVLLPAVGARAPAVGRVHFGELHGDD
                     AELSARLEADRAVPDSPPRVVRELEFAHLVNEADDALARGDVDVARASYLLALEQAPR
                     HAELSALIAEIDLRVNGRAEAALGLLVESLPATQAGSVGAELLARLGDFLGAREAVER
                     SASHEPFAPLAALQWLRLSELVSDVRQRQLALERAVARAPTLPETRWARFHARVSWGD
                     WQGAISDAEHLEASETGARGRHRACVDAADALLRAGNVQFAGRLFERALRYLPDDPGA
                     TAGLARALVQAKKPRRALVLFERAIELQERAGQLEARTWIELARLLAEQAQDLPQAIA
                     RLQQVSGAPGDIAEARHLEALYRARLGDRVGAVLAFARMREAIELTSSPEARFVPWLR
                     EAADQVFSVEADPAAAERHLAVALRLAPQDSELGSHYRQVAAALAELNVKSR"
     gene            1983..2357
                     /locus_tag="VFQ61_27515"
     CDS             1983..2357
                     /locus_tag="VFQ61_27515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958286.1"
                     /translation="MNRWMLLPCLLLAVAPACRETQECERARMNLAKTWKGLRESAAR
                     KKLAGGDVPSWQYVEQRADLLESAFTTSQVTWPSADKATTELAAKLTGMRVEEGDMSV
                     TAFRSSVQGAFQEQATYAKLCR"
     gene            complement(2388..3857)
                     /locus_tag="VFQ61_27520"
     CDS             complement(2388..3857)
                     /locus_tag="VFQ61_27520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958287.1"
                     /translation="MPTLPNPANPDPTAEQADSEDGDGPDEELPGLLDPMPAWDTREE
                     DADSGNDFLDWDLERGLERPSQDDEEEGVDIGSETQDLSPLPNEGSADEPVSDYSRRR
                     DEEELGVGEVGTDWLTEDSGQSALDDEAEGLEEIDLEQPELPNLQPEDESELPVHGEL
                     PLATAFADEARPRPASPPWALLEPPLELESCAAIATTDGVVVAASSDLLWFSPGELGP
                     LRLEAGSTPVHSVALLGTNWDLAICATESGKLLRRGRLSSASEVLRGAREAGDSAARQ
                     TLDLCQPGTGFPHALLVRTANGRLFRSDDDGATLRPLNTPKVVAVAPRAAVALSPGLL
                     LQSYDAGGSFEERALSGLARELSLAPAPLLAVCKNVIALAEPSLGVVISQDSGRTFQR
                     IPGSTNANALAVGEQDGELCVFAALYDGARDRSTLIRIYGIPRSAEEIARIEASEPGG
                     ASEDAEEGRVAQLVWDATQQRLWAVGGFGVRIYEPRRTQ"
     gene            3972..4229
                     /locus_tag="VFQ61_27525"
     CDS             3972..4229
                     /locus_tag="VFQ61_27525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958288.1"
                     /translation="MVAVRLRVRARDAVFVKGVLEASEGLGVLFAESGGDLLLAAPHT
                     LAAELEGVARELAEELGGVVEGDPRCHPGENAFRVLGRDHE"
     gene            4222..4923
                     /locus_tag="VFQ61_27530"
     CDS             4222..4923
                     /locus_tag="VFQ61_27530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958289.1"
                     /translation="MSEPPERRTPTVPISAEGNPLPRTSRSEGRLVGAWHEWLRALAT
                     DAEAALAAAIAYQELDAAGRNEWLTALDQDMDQVPVPKFAVYAPLLAVESDPERRARI
                     QRAVGPLDPTPARAVAHGYQGTLARGVRLAVLVSPLYLDFVQVLACAYRPDVGFEWVR
                     HDPIVERRRAPTAGAEIEGARIEAIAMRELIDELSHAVLAQQRSGQDLPEALRIFADL
                     FSPEPSGSWPVVSEC"
     gene            5017..5499
                     /locus_tag="VFQ61_27535"
     CDS             5017..5499
                     /locus_tag="VFQ61_27535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HET9958290.1"
                     /translation="MFAIGSCASDPNSQAAHPAAASGGPIHFAFGSLDGQVVTSDGTR
                     GRLTALLFVTTYDLPSHVAARRLNDCFRSHVPRFNAASIALESLENAPLVETFRDSLA
                     LRYPVALADQQELRASPAFAGIDRVPVLVLLDRSGRERFRHAGLFEAADLESWLRALE
                     "
     gene            5564..6607
                     /locus_tag="VFQ61_27540"
     CDS             5564..6607
                     /locus_tag="VFQ61_27540"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9958291.1"
                     /translation="MTAKNERAVRELPPVDESKSHDASALAETAPLPARISNIRLGTA
                     GFTDRTLIESRSFYPRGVTSSEARLRHYAAHFPLVEIDATYYSLLPPSSAERWTQNTP
                     PDFRIDVKAFPLFTEHPIDVRKLPQDLRQPLVSLGHEQRVYPKDLPPELAAEMANRFL
                     ALLEPLRAAGKLGAVLLQFPPWFTATRGNAQRIEALRTAYPQVPFAVEFRHGSWFESA
                     RQERVFSLLARHAMSYVSVDQPNLPFLPAVTHPELAYLRFHGHNVEGFGKRGASVIER
                     FCYLYSPQELAPWVAPVRRLAGNAREVHVLFNNCVRNFAVLGAKDLAALLAQEPSSAL
                     SRAETVSDGGAPC"
     gene            6618..7238
                     /locus_tag="VFQ61_27545"
     CDS             6618..7238
                     /locus_tag="VFQ61_27545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958292.1"
                     /translation="MLRSRKCRSALSFPSLQQVRRRLLPPLLVCGAVLGLAGSAAADV
                     SSWMYAGAGAAMLKGDRDSNRGTLQFDAGIGGPPGPVVLGAVFRFQPYLGKGTDLALL
                     GRVATGGYVQGNFGLAFDIGAYQRFWGVGSTGGLASLSLGAPWGITLSGSAGLGTEEQ
                     RFGSLTLGIDFARLTVHRTTGTSWFANPYATDEQGRGPRGPSSTRP"
     gene            7322..7972
                     /locus_tag="VFQ61_27550"
     CDS             7322..7972
                     /locus_tag="VFQ61_27550"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET9958293.1"
                     /translation="MAAQSFTRNHRDHSNDHGFQFEFFCDKCGNGHRSSFKTSAVGVA
                     ASVLKAAGAIFGGGMWRAGHGADHVKDALRGPAWDEAFAEATAECRPKFRQCTLCGKW
                     VCPEVCWNAERGLCEDCAPNLTEHAPALQARAAIDQARQKIEQADQIRGADVANAALS
                     LTPCTRCHAPLATGARFCAQCGTPAAAAGPKFCSGCGGALASGARFCPACGVAVTS"
     gene            complement(7991..9349)
                     /gene="gcvPA"
                     /locus_tag="VFQ61_27555"
     CDS             complement(7991..9349)
                     /gene="gcvPA"
                     /locus_tag="VFQ61_27555"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET9958294.1"
                     /translation="MRYLPHTPEEIQDMLSTIGRGSLDDLFATVPEAVRFQGSLDVEP
                     ALDEASLMSHLEDLAGKNTGARWLSFLGAGMYAHHIPPAVDQLLLRSEFYTAYTPYQP
                     EVSQGTLQVIWEYQTLVSELLGLPLANASMYDGASAAAEAALMAQRLTKRSRVLVSQC
                     VHPDYRDTIRTYLGGRDARAYVELPVNESALADSAGLIGALDADTAAIVVGYPSFFGS
                     LGDLRELARAAHAKGALLVTATTEPYALALAESPGALGVDIAVAEGQPIACPPQLGGP
                     GVGLFACRDDRSYLQQLPGRVCGETVDSKGQRGYVLTLSTREQHIRRERATSNICTNQ
                     GLIALALTIRMSLLGKSGFVRTAEQCLAKAHYLQQKVGSLAGYALAFSEAPHFNEFAV
                     KVRGGSAHAVLGHLEKQCIVGGYDLGRVAPELADKLLIAVTERHRRADLDRLVQALDS
                     FA"
     gene            complement(9349..9753)
                     /gene="gcvH"
                     /locus_tag="VFQ61_27560"
     CDS             complement(9349..9753)
                     /gene="gcvH"
                     /locus_tag="VFQ61_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET9958295.1"
                     /translation="MSKATDVKTDRKYTKDHEWALSQGNEVLVGITAFAVDQLGDVTL
                     VNLDVKVGDTVEAHKNFGTIESVKTLSDLFAPISGRIVRINAELENSPELINEDPWGR
                     GWMIAIEPSKPSEDAGLLDSAAYSALLESSSH"
     gene            complement(9757..10869)
                     /gene="gcvT"
                     /locus_tag="VFQ61_27565"
     CDS             complement(9757..10869)
                     /gene="gcvT"
                     /locus_tag="VFQ61_27565"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET9958296.1"
                     /translation="MDSALKRTPLHAEHARLGARLVPFAGYEMPIQYVGITEEHHAVR
                     RRVGIFDVSHMGEISITGPGALEAVDSLIVSDLTKTQDGQALYTCACNEEGTILDDLI
                     VYRRSATDILVVCNASNREKMVPVFETRQSNSATVRDVSDDFALLAVQGPSALALLAT
                     AGAGDALSGLPSFHFTEAELGGVRCTLARTGYTGEDGAEIFVPPSLAAQLFRHLLDQG
                     ASLGVMPVGLGARDTLRLEARLSLYGNDIDETTNPLEAGLGRLVHLNKPEFVGKAALT
                     RVSERGLTRRLVGFEMTGRGIARHGYPLGDASGATFGICTSGGPSPTLGKNIGLGYVP
                     LAAAAVGTEISVDCRGKRVSAVVVRTPFYKRPAKKD"
     gene            11025..11795
                     /locus_tag="VFQ61_27570"
     CDS             11025..11795
                     /locus_tag="VFQ61_27570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9958297.1"
                     /translation="MSKGRVLVIDGDEWSRTLLVNYLGAAGYEVHTAAGAREGFDRVR
                     ALEPDCIVCDVALPDIDGFWVARRVRAEASKVAATPLLFLTQADDLPGRLHGLKVGAD
                     VFLTKPVRSEEIVAQVDAVIGMASRILSASVPPTEAPPPSSRPPALAGDLSQISIATV
                     FTLLELEQRSGLLRVRNNETSGIDFELSQGALCSAKRSTNFARKHEEPKLLLHEVLNY
                     GHGTFKFRPGKVEKAPLQQKLSALLLDAMRLHDEDGGW"
     gene            complement(11834..12403)
                     /locus_tag="VFQ61_27575"
     CDS             complement(11834..12403)
                     /locus_tag="VFQ61_27575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958298.1"
                     /translation="MSAQRTSARRTSAKLAAATGLCAALALFVSPACRRSPETAGVPS
                     ASASAAQRPPLDRLGNNELAPGEEALFGLRLPRGMTVQGRFKDSGLAIGDLPVATVVS
                     YVKDHVEANHVELGATQTVFPSARVAGEANAPLLRIEIAAEAGVTRLHVRDMTPRPAP
                     RLDGLSNDDLWRRAGFDRNGKPLHPKELE"
     gene            complement(12430..>12976)
                     /locus_tag="VFQ61_27580"
     CDS             complement(12430..>12976)
                     /locus_tag="VFQ61_27580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958299.1"
                     /translation="KVNWFQEMTNGERVVGPMTLFNAQLFFSSYAPPGSTSHACSAGT
                     SKVWGVDYVRPLGFASQASPPRNRGGEARLPNPSGGFLQSLTAQAVAGSSDAVIFGVS
                     VAQEPTCSTNVENPQNDFLGYGATQAITNINPGRFQLVMHVGGVENTGAAGDLQANVK
                     TVNLQSPGNATRVDSWAALVE"
BASE COUNT         2055 a         4164 c         4340 g         2417 t
ORIGIN      
        1 aagagacatc gaggccgatg ccgtggaaca ggcgtcggcc tcgatcgtct tggcggacga
       61 tcagcacgtc ccgctgcgcc ttacggcttc gagaggcacg ctgggcctcg agctctacgc
      121 cccggtcgag attgggccgc tcaagatcac ttcgttgctg ttgagcctcc ccgggttgaa
      181 gtttcccctc gatctttcgg gcggagttcc gaaatttcgc catcgtcggg gcgagctgca
      241 agaaatgacg ctggggctct cgttcgcggc gctcgcgcgc tggctcgggg cgcgctcggg
      301 tgagctgact cagggcctgt cacgcgcccc ggcgctgttc cgggcgccag acgggctgac
      361 ggtggggctc gtgtgggggc gccgagcttt ggcattcgat ttggtgtggg ctccggatcg
      421 cgatgccgca cgctgcgtga tcacgcgtgc acgagggatg ggtctcgatt tacccgcact
      481 gggtctcgct ctgcgagtcg cagatacgtg gtttggagcc tgggcggagc gtcgcggacg
      541 agtgatccgc tttccgaacg taccgcaacg ggttgggcga gtactgctcc cggccgtggg
      601 tgcacgcgcg ccggcggtgg ggcgcgtgca ctttggcgag ctgcatggcg atgacgccga
      661 gctctcggct cggctcgagg cggatcgtgc ggttccggac agcccgccgc gcgtcgttcg
      721 ggagctggag ttcgctcacc tggtgaacga agccgacgac gcgcttgctc gaggtgacgt
      781 cgacgtggcc cgggcctcgt atcttttggc gctcgaacag gctcctcgcc acgccgagct
      841 gagcgcgctg attgccgaga tcgacttgag agtgaacggg cgcgccgaag cagcgctcgg
      901 cttgctcgtc gagagcttgc cggcaaccca agcgggctcg gtcggggccg aactgttggc
      961 gcgtctgggg gattttctgg gtgcacgaga agccgtggag cgcagcgcca gtcatgagcc
     1021 ttttgcgccg ctcgccgcgc tgcaatggct acgtttgagc gagctcgtca gtgatgtgcg
     1081 tcaacgccag ttggcgttgg agcgagcggt agcccgcgcc cccaccttgc cggagacgcg
     1141 ctgggcccgg tttcacgcgc gagtcagctg gggagattgg cagggagcca tttcggatgc
     1201 cgagcatctg gaagcgtccg agacgggcgc gcgcggacga caccgggcgt gcgtcgacgc
     1261 ggcggacgcc ttgctacggg cgggtaacgt gcagttcgcg ggacggttgt tcgagcgcgc
     1321 gctgcgctat ctgccggacg accccggcgc gaccgccggc ctcgcgcgcg cgttggtgca
     1381 agccaaaaag cctcgacgcg cgctggtgct gttcgaacgc gccatcgagt tgcaggaacg
     1441 ggcgggccag ctcgaggctc gaacgtggat cgagctggcg cgtctgctcg ccgagcaggc
     1501 ccaagatttg ccgcaagcca tcgctcgctt gcagcaggta tccggcgccc cgggagatat
     1561 tgcagaggcg cgtcacttgg aggcgctcta ccgggctcgc ctcggggatc gcgtgggggc
     1621 ggtgttggcc ttcgcgcgta tgcgggaggc catcgagctg acgagctcgc cggaggctcg
     1681 gttcgttccg tggctgcggg aggccgccga tcaggtcttc tccgtggaag ccgatccggc
     1741 cgcggccgaa cggcacttgg cggttgcgtt acgcctggcg ccgcaagact cggagctcgg
     1801 ctcgcattac cgtcaggtgg ccgccgctct cgcggagctc aacgtcaagt ctcgctaggc
     1861 gcgcgcggta gctcgaatcg cgcgagacct gaaaaacgcc gggcagctcg aatttcgcac
     1921 cagatgctca acgacgagcc cgccggcacg gctttgctct atcctgcagc gacaggctgc
     1981 ccatgaatcg atggatgttg ttgccgtgtt tgctgctggc ggttgctccg gcctgccgtg
     2041 agactcagga atgcgagcgg gcgcggatga acctcgcgaa gacgtggaag gggctgcgcg
     2101 agtctgccgc gcgcaagaag ctcgcgggcg gtgacgtgcc gagttggcaa tacgtagagc
     2161 aacgcgcgga cttgctcgag agcgctttca cgacctcgca agtcacctgg cccagcgcag
     2221 acaaagccac gaccgagctc gcggccaagt tgacggggat gcgcgtggag gaaggggaca
     2281 tgagcgtgac cgcgttccga agcagcgtgc agggcgcttt ccaagagcag gcgacctacg
     2341 ccaagctctg ccgttgagcc gttctattcg gttggtccgt ttggaggtta ttgggtgcgg
     2401 cgtggctcgt aaatgcgtac gccgaacccg ccgactgccc acaagcgttg ctgggtcgcg
     2461 tcccagacta gctgtgcgac gcgcccctct tccgcgtctt cgctcgcgcc gccgggctcg
     2521 gacgcttcga tgcgggcaat ctcttcggcg cttctcggta tcccatagat cctaatcagc
     2581 gtgctgcgat cccgcgctcc atcgtacaag gcggcgaaga cgcacagctc gccgtcttgc
     2641 tcgccgaccg cgagcgcgtt ggcattcgtg ctgcccggga tgcgctggaa tgtccgtccg
     2701 ctgtcctgcg agatgaccac gccgaggctt ggttccgcga gcgcgattac gttcttgcag
     2761 accgccagca ggggcgctgg cgccaggctc agctcccgcg ccagcccgct cagcgcgcgc
     2821 tcttcgaagc taccccccgc gtcgtagctt tgaagcaaca aacctggcga gagggcgacc
     2881 gccgcgcgcg gcgccacggc tacgaccttg ggcgtgttga gcggccgcaa agtcgcgccg
     2941 tcgtcgtcgc ttcgaaacag ccgcccgttc gcggtgcgga ccagcagcgc gtggggaaac
     3001 ccagtgcccg gctggcacaa atccagggtc tgccgtgcgg cgctgtcacc ggcttcacgt
     3061 gctccgcgta gcacctccga agctgaagag agccgacctc gacgcaacaa cttgccgctt
     3121 tcagtcgcgc aaattgccag atcccagttg gtccccagca gtgccaccga atgaacgggg
     3181 gtagagccgg cctcgagccg caacgggccg agctcaccgg gtgaaaacca caacagatcg
     3241 ctgctcgcgg cgaccaccac gccgtccgtc gttgcgatcg ccgcacagct ctccagctcc
     3301 agcgggggct ccaaaagagc ccaaggcgga gacgcggggc gtgggcgcgc ttcgtccgcg
     3361 aaagccgtag ccagtggcaa ctctccgtgc acgggcagct cgctctcgtc ttccggctgg
     3421 agattgggca gctcgggttg ctcgagatcg atctcttcga gcccctcagc ctcgtcatcc
     3481 aacgcgctct gtcccgagtc ctcggtcagc cagtccgtgc caacttcacc gacccccaac
     3541 tcttcttcgt ctcgacgcct ggaataatcg ctcacaggtt catccgctga accctcgttc
     3601 ggcagcggtg agagatcttg cgtctcgcta ccaatatcga cgccttcttc ctcgtcgtct
     3661 tgcgatggcc gctcgaggcc ccgctcgaga tcccaatcca gaaaatcatt tcccgaatcc
     3721 gcatcctcct cgcgcgtgtc ccacgcgggc atgggatcca acagtccggg cagttcctca
     3781 tcgggaccgt cgccgtcctc cgagtccgcc tgttcagcgg tcggatcagg gttagcgggg
     3841 ttcggcaacg tcggcatgta cggtcgaggg tagaaccgca ggcccagtga gtcgagctac
     3901 ggggctcgat ttctgactga agtcggctac cttcagcgcc gatgccggtg gcgccccttc
     3961 tagatgagga cttggtagca gttcggttgc gggtgcgcgc ccgcgacgcg gtgttcgtga
     4021 agggagtgtt ggaagccagc gagggcttgg gcgtgttgtt cgccgagtcc gggggcgatc
     4081 tgcttttggc ggcgccgcac actttggcgg cagagctcga aggtgtggca cgggagttgg
     4141 cggaggagct cggtggcgtc gtcgagggag acccgcgttg ccacccgggc gagaacgcgt
     4201 ttagggtgct tggccgcgat catgagtgag ccgcccgagc gccgaacccc gaccgtacct
     4261 atttcggccg agggtaaccc attaccgcgc acctcgcgat ccgaggggcg tttggttggt
     4321 gcatggcacg agtggctgcg ggccctcgcg accgacgcag aagcggcctt ggccgcggcc
     4381 attgcgtacc aggaactgga cgcggccggc cgcaacgaat ggctgacggc gctcgatcaa
     4441 gacatggatc aagtgccggt ccccaagttt gcggtgtacg cgccgttgtt ggcggtggag
     4501 tccgatccgg agcgccgtgc gcggatccag cgtgcagtgg ggcccctgga tcctacgcca
     4561 gcgcgcgcgg tggcccatgg ttatcaagga acgctcgcgc ggggcgtgcg cctcgcggtg
     4621 ttagtgtcgc cgctctatct cgacttcgtt caggtcttgg cgtgcgcata ccgtccggac
     4681 gtgggcttcg aatgggttcg ccacgatccc atcgtcgagc ggcgccgtgc gcccacggcg
     4741 ggtgcggaga ttgaaggcgc gcgtatcgag gcgatcgcga tgcgcgagct gatcgacgaa
     4801 ctctcgcacg ccgtgctggc tcaacagcgg agcggccaag acttaccgga ggccttgcgc
     4861 atcttcgcgg atctattcag cccggagccc agcggttcct ggccagtggt ctcggagtgt
     4921 taggtgcggg acggggcggc gtgctttcgg ccacgagaac ttgcagggaa gcgggaggcc
     4981 gcgcgcgctc tatcgcgccg gagtttgctg ctcggggtgt tcgcgattgg cagctgcgca
     5041 tcggacccca actctcaggc cgcccatcct gcagccgcga gcggcgggcc cattcatttc
     5101 gccttcgggt cattggacgg tcaggtcgtg acttccgatg ggacgcgcgg tcgcctgacg
     5161 gcgctgctgt tcgtcaccac ttatgatttg ccgagtcatg tcgctgcgcg ccgtttgaac
     5221 gactgctttc gaagtcacgt tcctcgcttc aatgccgcgt cgatcgcgct cgagtcgctg
     5281 gagaacgcgc cgctcgtcga gacatttcga gactctctcg ctttgcgtta tccggtcgcg
     5341 ctcgcggatc agcaagagct gcgcgcttcg ccagccttcg ccggtatcga ccgcgtgccg
     5401 gtgctcgtgc tgctcgaccg cagcgggcgc gagcgcttcc gccacgcggg gctgttcgaa
     5461 gcggcggatc tggagagctg gctgcgcgct ttggagtgaa ggggggggcg ggcggcgcgg
     5521 ccttggctcg caccgggcgc gcccttgccg taggctgtcg ctcatgacgg cgaaaaacga
     5581 acgcgccgtg cgcgagctgc cgcctgtaga cgagtccaaa tcccatgatg cttcggcgtt
     5641 ggccgagacc gcgcccttgc cagctcgaat ctcgaacata cgactcggaa ccgccggctt
     5701 cacggatcgc actctgatcg agagccggtc tttctatccc cgcggggtaa ccagttcgga
     5761 ggctcgcttg cgccactacg cggcgcattt tccgttggta gagatcgacg ctacctacta
     5821 ctcgctgttg ccgccctcca gcgcggagcg ctggacccag aacacaccgc cggactttcg
     5881 aatcgatgtc aaagccttcc cgctgttcac ggagcatcca attgacgtcc gaaagctgcc
     5941 gcaagatctg aggcaaccgt tggtcagctt gggtcacgag cagcgcgtct accccaagga
     6001 cttgccaccc gagctcgcgg ccgagatggc caatcgcttt ctggcgctgc tcgagccgct
     6061 gcgcgccgcg ggcaagctcg gggcggttct cctgcaattc ccgccttggt ttaccgcgac
     6121 gcgtggcaac gctcagcgca tcgaggccct gcgtacggca tatccgcaag tgccattcgc
     6181 ggtggagttc cggcacggga gctggttcga atcggcgcgc caagagcgag tcttcagcct
     6241 gctcgcgcgc cacgcgatga gctacgtgtc cgtcgatcaa cccaacttgc cgttcctccc
     6301 ggccgtcacc cacccagagc tcgcttactt gcgtttccac ggccataacg tggagggctt
     6361 cggcaaacgg ggcgccagtg tgattgagcg gttctgctac ctgtattctc cgcaagagct
     6421 ggccccctgg gtggcgccgg tccggcgtct ggccggaaat gcgcgcgagg tccacgtctt
     6481 gttcaataac tgcgtcagga attttgcggt actcggcgcc aaggacctcg ccgcgttgct
     6541 ggcccaagag ccctcgagtg cactctcacg agctgaaacc gtttccgacg gcggcgctcc
     6601 gtgctagctc gcgcacggtg ttgcgttcaa ggaagtgccg ttcggccctc tcgtttccga
     6661 gtctgcagca ggtgaggcgt cgccttctcc cgccgctgtt ggtgtgcggt gccgtactgg
     6721 gcctcgctgg ttcagccgct gccgacgtga gttcgtggat gtatgccggc gcgggcgcgg
     6781 ccatgctgaa aggcgatcgc gactcgaacc gcggtacgct gcagttcgac gcgggcattg
     6841 gcggccctcc cgggccggtg gtcctcggcg cagtgttccg gtttcagcca tatctgggta
     6901 agggcaccga cttggcgctg ctcgggcggg tggcgacggg cggctacgtg cagggcaact
     6961 tcggtcttgc gttcgacatc ggcgcctatc agcgcttctg gggcgttggc tccaccggtg
     7021 gcttggccag cctctctcta ggggcccctt ggggcatcac cctcagcggg agcgccggtt
     7081 tgggcaccga agagcagcgc ttcgggtcgc ttacgctggg catcgatttc gcgcgtttga
     7141 cggtgcatcg caccaccggt acgagctggt tcgcgaaccc ctacgcgact gacgaacaag
     7201 gccgtggccc gcgcggtccg agttcgacgc gtccttgagt ggctgagtaa tcgttcgagc
     7261 gctcgtgcgc ttcgcgtaac ggacgctggc ctcggctttt ctggggcagt atcgttttcg
     7321 aatggcggcc cagagtttca cgcgcaatca ccgcgaccac tcgaacgatc acggctttca
     7381 attcgagttc ttctgcgaca agtgcggcaa cggtcaccga tcgagcttca aaacgagcgc
     7441 ggtcggcgtg gcggcgagcg tgctgaaggc tgcgggggca atctttggcg gcggaatgtg
     7501 gagggcgggc cacggtgccg atcacgtcaa agacgcgctg cgcggcccgg cttgggacga
     7561 ggcgttcgcc gaagcgaccg cggagtgccg cccaaagttt cgtcagtgca cgttgtgcgg
     7621 caagtgggtg tgccccgagg tgtgttggaa cgcggagcgc gggctgtgcg aggactgtgc
     7681 gccaaacctc accgagcacg ctccggcgct gcaggcccgc gctgccatcg accaagcgcg
     7741 ccaaaagatc gagcaagcag accagatccg cggagcagac gtcgcgaacg cagcgctctc
     7801 acttactcct tgtacgaggt gtcacgcccc gttagccacg ggcgcacgct tctgtgcgca
     7861 gtgcggcacg cccgcagccg ccgcgggtcc gaagttctgc agcggctgtg gtggtgcact
     7921 ggcttccggt gcgcgattct gtccagcctg cggcgtcgcc gtcacaagct aggctgacgg
     7981 cccgagcgcg tcaggcgaac gagtctagcg cctgtacgag gcgatccaag tcggcgcgcc
     8041 ggtgcctctc ggtaaccgca atcaagagct tgtccgcgag ctcgggtgcg acgcggccga
     8101 ggtcatagcc gcctacgatg cactgctttt ccagatggcc gagcaccgcg tgggcgctcc
     8161 ctccgcgcac cttgaccgcg aactcgttga agtgcggcgc ctcagagaac gctagcgcgt
     8221 agcccgcgag tgagccgacc ttttgctgca agtagtgcgc tttggcgagg cactgctccg
     8281 ccgtacgcac gaagccactc ttgccgagca aactcattcg gatcgtcagc gcgagcgcga
     8341 tgaggccctg attggtgcag atgttgctgg tcgcgcgctc gcgtcggatg tgctgttcgc
     8401 gcgtcgagag cgtgagtacg tagccacgtt ggcctttgct atcgaccgtc tctccgcaaa
     8461 cccttccggg taattgctgc aagtagctgc ggtcgtcccg gcaagcgaac aggccaacgc
     8521 cggggccgcc gagctgcggc gggcaggcga tgggttgccc ctccgctacc gcgatgtcca
     8581 cgcccagcgc acccggggac tcggctagtg ccaacgcata cggctcggtc gtcgccgtca
     8641 cgagcagcgc tcctttggcg tgggcggctc gagcgagctc gcgcaaatca cccagtgagc
     8701 caaagaagct cgggtacccg accacgatgg ccgccgtgtc ggcgtccaac gcgccgatca
     8761 gacccgcgct gtccgcgagc gcgctttcgt tcacgggcaa ctcgacgtag gcccgcgcgt
     8821 cgcgcccgcc gaggtacgtg cgaatcgtat cccgataatc cgggtgcacg cactgactca
     8881 cgagtacgcg ggagcgcttc gtcagccgct gtgccatcag ggccgcctcc gccgccgcgc
     8941 tcgcgccgtc gtacatgctg gcgttggcga gcggtaagcc caaaagctcg ctgaccagcg
     9001 tttgatattc ccaaatcacc tgcagggtgc cttgagatac ctcgggctga tacggcgtgt
     9061 acgccgtata aaactcgctg cgcagcagca gctgatcgac ggcgggcggg atgtggtgcg
     9121 cgtacatgcc cgcacccagg aagctcaacc agcgcgcgcc ggtgttctta ccggcgaggt
     9181 cttccaggtg actcatcaag ctcgcttcgt cgagcgcggg ttcgacgtcg agtgagccct
     9241 ggaaccgcac ggcctccggc accgtcgcga acaggtcgtc caagctgccg cgcccgatgg
     9301 tgctcagcat gtcttggatc tcttcggggg tatggggcag gtagcgcatc agtgagagct
     9361 gctttccaag agcgcggagt aggcggcgga atcgagcaaa ccagcgtcct cactcggctt
     9421 gctcggctcg attgcgatca tccaacctcg gccccacggg tcctcgttga tcagctccgg
     9481 gctattctcg agctcggcgt tgatgcggac gatgcggccg ctgatcggcg cgaacaggtc
     9541 gctcaaggtc ttcacgctct cgatcgtgcc gaagttcttg tgcgcctcca ccgtgtcgcc
     9601 caccttgacg tcgaggttga ccagcgtgac gtcgccgagc tggtcgaccg cgaacgcggt
     9661 gatgccgacc aagacctcgt taccttggct gagcgcccac tcgtggtcct tggtgtactt
     9721 gcggtcggtc ttgacgtcag tcgctttgct catccttcag tcctttttcg cgggtcgctt
     9781 gtagaacggc gtgcgtacca cgacggccga tacgcgcttg cctcggcaat ccaccgagat
     9841 ttcggttccg accgcggccg cggctagcgg aacatagccg agcccaatgt tcttgccgag
     9901 agtcggcgag gggccgccgg acgtgcaaat accaaaagtc gcgcccgaag catcccccag
     9961 cgggtagcca tgacgcgcga tgccccggcc ggtcatttcg aaaccgacca ggcgccgggt
    10021 cagacctcgt tccgacaccc gggtcagcgc cgcttttccg acgaattcag gcttgtttag
    10081 gtgcaccaga cggccgagcc cggcctccaa aggattcgtg gtctcgtcga tgtcattgcc
    10141 gtagagggac aggcgtgcct cgagccgtag tgtgtcgcgc gcaccgagac ccaccggcat
    10201 caccccaaga ctcgcgcctt gatcgagcaa gtggcggaac agttgcgccg ccaggctggg
    10261 cgggacgaag atctcggccc catcttcacc cgtgtagccg gtgcgagcca aggtgcagcg
    10321 cacgccaccc agctccgcct cggtgaaatg gaacgagggg agtcctgaca gcgcgtctcc
    10381 cgcccccgcg gtcgcgagca acgccaatgc gctcgggccc tgcaccgcga gcaacgcgaa
    10441 gtcatcactc acgtcgcgca ccgtcgcgct gttgctctgc cgcgtctcga agaccggcac
    10501 catcttctca cgattggatg cgttgcagac gacgaggatg tcagtcgcac ttcggcgata
    10561 gacgatcaaa tcatcgagga tcgtgccctc ctcgttacaa gcgcaggtgt agagcgcttg
    10621 gccgtcctgt gtcttcgtca aatcgctgac gattagactg tccaccgcct ctagcgcgcc
    10681 ggggccggtg atcgaaattt cccccatgtg agaaacatcg aagatgccga cgcgccttcg
    10741 cacggcgtgg tgctcctcgg tgattccgac atattggatg ggcatctcgt agccggcgaa
    10801 gggcacgaga cgagcgccga ggcgagcgtg ctcggcgtgg agcggtgtgc gtttgagtgc
    10861 agaatccacg ggtcaccttc gtaccctggg caacacagcc gcacaacccc tcaattgtgc
    10921 gccgcgtgcg ctgtctgctc cgctccgcac atcacggttt tttcgagggc ttctctgtag
    10981 tggggctgct ctccggggcg cgtgtccgtg ctagcgtggg agtggtgtca aagggccgag
    11041 tgctcgtcat cgacggcgac gaatggtctc ggaccctgct cgtcaactat ctcggtgcgg
    11101 cgggctacga ggttcacacg gccgcgggcg cgcgggaagg cttcgatcgc gtgcgcgcgc
    11161 tcgagcccga ctgcatcgtt tgcgacgtgg ccctgccgga tatcgacggt ttctgggtcg
    11221 cgcgtcgggt gagggcagag gcttccaaag tcgcggccac gccgctgctg tttttgaccc
    11281 aagccgatga tcttccgggt cgactgcatg ggctgaaggt tggggccgac gtatttctga
    11341 ccaagccggt gcgcagcgaa gaaatcgtgg ctcaagtgga cgcggtaatc ggcatggcga
    11401 gtcggattct ctcggcctcg gtgccgccga ccgaagctcc ccctccgagc tcccggccac
    11461 ccgcgctggc cggagacttg tcgcaaattt cgattgcgac cgtgttcacg ctgctcgaac
    11521 tcgagcagcg cagtgggcta ttgcgggtgc gcaacaacga gacttcgggc atcgatttcg
    11581 agctgtcgca aggcgcattg tgctcggcga agcgctccac caactttgcg cggaagcacg
    11641 aagaacccaa gctgctgctg cacgaggtgc tcaattacgg ccacggcacg ttcaagtttc
    11701 gccctggcaa agtggaaaag gcgcctttgc aacagaagct gagtgcgttg ttgctcgatg
    11761 ccatgcggtt gcatgacgag gacggcggct ggtaactacc gcacggcggc cgttctcgat
    11821 gcttttggct gtactactcg agctccttcg ggtgcagcgg cttgccgttt cgatcgaagc
    11881 cggcgcgacg ccacaaatcg tcgttactca ggccatcgag gcgaggggcg ggacgcggcg
    11941 tcatgtcccg tacgtgaagc cgcgtcactc cggcctcggc cgcgatctcg atccgcagca
    12001 aaggagcatt ggcctccccg gccactcgcg ctgaagggaa cacggtctgg gtcgcgccga
    12061 gctcgacgtg attggcctcg acgtgatcct tcacgtagct cacgaccgtc gcgaccggca
    12121 ggtccccaat cgcgagcccg ctgtctttga aacggccttg cacggtcatg ccgcgaggca
    12181 ggcgtaaacc gaacaacgcc tcctcgccgg gcgcgagctc gttgtttccc aaacggtcga
    12241 gcggcgggcg ctgggcggca gaagcggaag cggacgggac accagctgtc tctggtgagc
    12301 ggcgacacgc cgggctcacg aagagcgcca gcgccgcgca taacccggtg gcggcggcca
    12361 gcttcgcgct cgttcggcgc gcactcgttc gctgggcgct cactgcgcta gcgtttcctc
    12421 tactagcact cactctacca gcgccgccca tgagtccacc ctcgttgcgt tgcctggtga
    12481 ctggagattg acggtcttca cgttggcttg aagatcgccc gcggcgcccg tgttttctac
    12541 gccgccgacg tgcatgacga gctgaaagcg cccgggattg atgttggtga tggcctgggt
    12601 cgccccgtaa ccaaggaaat cgttctgcgg attttcgacg ttggtgctgc acgtgggctc
    12661 ttgcgcgacc gacacgccga agatgacggc gtccgagctg ccggccacgg cttgagccgt
    12721 gagcgattgc aggaagccgc ccgaagggtt gggcaggcgt gcctcaccgc cgcggttgcg
    12781 gggaggcgag gcctgtgagg cgaacccgag tgggcgcaca tagtcgacgc cccagacctt
    12841 gctcgtgccc gcgctgcacg cgtgggacgt ggagcctggg ggcgcgtagc tggagaagaa
    12901 gagctgcgcg ttgaacaacg tcatgggtcc cacgacgcgc tcgccattag tcatttcctg
    12961 gaaccaattg accttg
//
LOCUS       DASTKU010000787        14696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1495709, whole genome shotgun sequence.
ACCESSION   DASTKU010000787 DASTKU010000000
VERSION     DASTKU010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14696
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495709"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VFQ61_27585"
     CDS             complement(<1..1096)
                     /locus_tag="VFQ61_27585"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9958300.1"
                     /translation="MREPDIVVIGSGPNGLSAAIKLATQGLRVLVLEENSRRPGGAVG
                     SDEATLPGFVHDLGAGFFPLARLSPAFREFKLERFGLEWLNAEIESCHPALDGSSASL
                     IRLAGSDAMGFRAEGLGDEYFGSEADTRTFQRLAAEYASNEAALVAALLGPLPGLRAF
                     ARLGLRGLLRLGLRFTQSTRRLSNRWFSSEAARRVLPGLAMHADLGPDAFAGAAMAYM
                     LAFSATTVGYSVARGGAQRLTDSLVTALELSGGQLQLGARARRIVVRDRRAVAVQLQT
                     GEEISFRHAVLADTSPGQLLLELLPESTVPPYAIKAARSFRQGYGTFKIDYALGYPVP
                     WRDANARRSATVHIGESVDDLARFVRELEAG"
     gene            1200..2345
                     /gene="ald"
                     /locus_tag="VFQ61_27590"
     CDS             1200..2345
                     /gene="ald"
                     /locus_tag="VFQ61_27590"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET9958301.1"
                     /translation="MTECRRWSQAEVKVIVGVPKETKQHEYRTALLPVGVEELKRAGH
                     RVLVQRGAGLGSGVRDAEYEKLGAELIDGPEGVFAGADLIVKVKEPQPAEIPMLRAGQ
                     LVFTYFHFAASESLTRGVLASGATAVAYETLQDSRGSLCLLTPMSEVAGRMSIQQGAK
                     YLERPQEGRGILLGGVPGVEPAHITVIGGGVVGTNAAKIAAGFGAAVYVLDTNLDRLR
                     YLDDIMPPNVTTLFSDRHTLLDQLTKADLVIGAVLSRGARTPRLVRREDLAQMKQGAV
                     VVDVSVDQGGCFETTRPTSHADPTYVVDGVVHYAVTNMPGAVGRTSTYALCNATLPYV
                     LKLARLGLEEAVREDPGFESAVNIHRGEVTHPAVAETFGLPYRALFT"
     gene            complement(2377..3129)
                     /locus_tag="VFQ61_27595"
     CDS             complement(2377..3129)
                     /locus_tag="VFQ61_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET9958302.1"
                     /translation="MFPVRHAWLPAIASAACFSFLMLALPGLSEARKAPRESECRPQK
                     ALDFLKRPSFVKKGRLDGKAADRALQYRVEHYGRVDGVGFAELNSTPARSQATTVRFF
                     GLPVSVHLAIRPALECVEKRIRNTCTSRKEVYEPKALGGFRDINKYRGGEVSNHLFGI
                     AVDIDPDRNPCCGCVDPWPQHRACRTGNGDAFKETELPACWINAFERYGFYWLGRDPQ
                     LRDTMHFEFLGNPERFVRGATRDRVAHSKRRP"
     gene            3353..3946
                     /locus_tag="VFQ61_27600"
     CDS             3353..3946
                     /locus_tag="VFQ61_27600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958303.1"
                     /translation="MVVVRHETRAGFWHSNRRIFQQLFALAVLGIVGLPGTSCASHAE
                     CTPPIPSGVTYRVRVAPVLPQGQAAGCHVIDLTKSITPNQFEITTGRAAPTPSRPDCS
                     LTPLQDPPVQSSVKITRCVPYEAEMLGLSCDIEYAPMCPGRMNFFFRPVAGETVNWDA
                     PTISNLEFVMSDRTQGCLIGTQNCDDVYYAQLERIVN"
     gene            4134..5528
                     /locus_tag="VFQ61_27605"
     CDS             4134..5528
                     /locus_tag="VFQ61_27605"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET9958304.1"
                     /translation="MFPLHIAPPRGGGESDRTSETSALAITRDDVRIRDLGRCLLPSP
                     VARHLGDHALHFVGEADKVLVNDTLSHLEAYRGELDTLPAFELAGPRNKIFFDPRATR
                     VGIVTCGGLCPGLNNVIRGLVLELSRSYGVKRILGFRFGYAGILSRFGEAPVPLTPES
                     VAHIHHQGGTILGSSRGAQDPVEVVDNLEALGIDLLFVIGGDGTIRGAMQLAREIERR
                     RARIAVVGVPKTIDNDIHFIDRSFGFESAYAASVEVIRAACVEARGAARGIGLVKLMG
                     RHSGFVACHAALASTDVDLVLIPEVPAVLEGERGVLAQLEKLLEASGHAVVVVAEGAA
                     QELIESDSLARSHDESGNQKLKDVGIFLRDRITQHFATKSRPVTLKYIDPSYSIRSVP
                     ASPSDSVYCWNMARNAVHAGMAGNTEMLIGRWHGRFVHVPMELATRTRKQVVPGDELW
                     CSVIESTGQPRAFV"
     gene            complement(5561..6886)
                     /locus_tag="VFQ61_27610"
     CDS             complement(5561..6886)
                     /locus_tag="VFQ61_27610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958305.1"
                     /translation="MSPWIVPILAQALSLSVGDRTEARLRVETNDRVPEAETRPYADL
                     RWSGRRSALSFGYSPLFVLSPLDEKKPELYVLHTARIGFDFRQRRTAYQITQTASYGR
                     QNFASTALSGTPGLPAGTFPATPGTGPGGTVTTPTGTPGTTTGNGTPLGPGTTTGPGA
                     VPGTATGTPIGATNPNSAAVRDALTVAVLRTDFTATHALTRTSSFTFGAGYFVIGGVG
                     DSRRELPLQSGPDLRLRYDLRLTAKDQLFTATTGTYSSVSNGTRLWRLSLEQGYAHQF
                     SESILVRLSAGVSYVRERRDGVWTDTIYPTFGSNALVGVSYTTRFAGGQLTASAGVGY
                     SPALTYTPTTKNIDVRIDPRFLMFGGLGWVKHRTTLFLNAGATLSLTPSEPGSVSNYT
                     GAAGMYYTIVDGLVFEAGLRGLRQTVANGPDISFAYALYAALRYDTTLF"
     gene            7540..8049
                     /locus_tag="VFQ61_27615"
     CDS             7540..8049
                     /locus_tag="VFQ61_27615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958306.1"
                     /translation="MEKTTALARSEDNLAISDLWREAEWQRLWLAVQAQQWSSLALIP
                     AAPGAPEDFALTIAVTLAKTGIVHLGVPIHVADGARIQMADLATFGQEVARCKEYGDR
                     VLMALAPIDENPVSVTLAKSADFALLCILLEDMDSSQSKKTVAAIGQNRFIGSVMFHP
                     SQLNGAARR"
     gene            complement(8099..9745)
                     /locus_tag="VFQ61_27620"
     CDS             complement(8099..9745)
                     /locus_tag="VFQ61_27620"
                     /inference="COORDINATES: protein motif:HMM:NF024295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HET9958307.1"
                     /translation="MRRHRRALIQLLTGLVGGLALVASAGWLCFPLRDEPVRAAASPR
                     VVSSRRPDVLKVRCSLPARPIHPGIYGFAAGDYVHESARRIGGNPMTRLNFELGDTWN
                     AGSDWFFENVRGDSALDDWLEDGLSRGLSMALTVPMIGWVAKDGSSFSFPVSVFGPQQ
                     HTDPNRPDAGNGLRGDGEPIAPGPASRTSIAAPPEKIGAWIRALRAADRARGVRRVHM
                     YILDNEPDLWHVTHRDVHPEPLSYDELIERTIAYATQIRAADPDALIAGPASWGYVGY
                     FDSPRDTTSRLGLRLDRRRHGGLPLLAYYLQALAAHERRTGTRLLDVLDVHFYPQADG
                     VYANSRTDAATAALRLRSTRALWDPSYVDDSWIEAPIQLIPRLRQWVERYYPGRALSL
                     GEWSFGAEDHISGALATAEALGRFGQHELDWAFYWRGPRSGSFTFEAFRAFRDFDGAG
                     ARFLDWSVPTQATERISLFASRNAESRTLVLVLIQRDATHRAEMSLDLSECGEVLAAR
                     RFVLRSNVPRLEAADASMLRGRTLPLDLSPLSLEVLELTL"
     gene            complement(9782..10846)
                     /locus_tag="VFQ61_27625"
     CDS             complement(9782..10846)
                     /locus_tag="VFQ61_27625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9958308.1"
                     /translation="MRTLILAAGRFGEQLRRDIAKGREPRLDVFELSNVLRADVIDFL
                     DVERSNQKRVKLTQKSLGYSAAVAYLGFLERKNYDAILTSGEDIGLPLCALLARSSAR
                     CSHTMIAHTLFPRKKRVFFQYLNAATRLDRILVYSTSEEQLVTGELGVPRMKVKRIHY
                     HADQQFFRPVERPVEPDLICAAGQLLRDYDCLVEAVRDLPVRVQIAAGSPWIERKLEP
                     QKPLPGNVSWGKLNRFDLRDLYERAAVAVVPILENHYQTGIATILEMMAMGKCVIATR
                     TQGQTDTIVDGQTGIYVPPSDPVALRTQIQRVLRNPDEAQRIGQAARKFVEEHAGLDQ
                     FVTAIAAAVREGFEARASSS"
     gene            11273..12748
                     /locus_tag="VFQ61_27630"
     CDS             11273..12748
                     /locus_tag="VFQ61_27630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958309.1"
                     /translation="MATERDEDDEEGGGSAQPDLIQSYLSFAARAIRSRIPLVLGTIA
                     VVSLLTVAAAAVWPRTYSSLTQIMVQSQNIPGVQKDDPFTGADRVIMRRENIEALVKQ
                     TELTKHWQRGRSPALLLKDRLMELIRGPIPESALAPVLVSTLESRIYPDASDRTLTIT
                     VDWSDPELAAKIAETAMKRFVDFRQTAEIASRQKEIDLLEAHAADTRKRLKERADELK
                     KRREERLANREPDPVPVPGEAPKPKVVRTRPQRDLAAEGELATLRADLTARRGQQMER
                     ERQDQSQVNELRSKLDETLRVVTRSHPDAVIAQRRLDAAMAAAAANKSQGTQDLADLE
                     RRVKEAEGRVVAGGGSIAVIGDSGSATPSVPPEIRRLIEDSSEEGVDPTLISEVEHQA
                     AKLTDLRDQIDKESLNLQRAQQAFETRYQVVIPAEIPGKPKKPNVRMIIAGGVIGSIV
                     LGLVLAILAQLRKGKIVAKWQVYNLGVPVLAEIKFPRGPNA"
     gene            12762..14021
                     /locus_tag="VFQ61_27635"
     CDS             12762..14021
                     /locus_tag="VFQ61_27635"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9958310.1"
                     /translation="MTLWSGFSALSGLVGALAVPTGLLTGYLGALTALSAPIEPPKAS
                     DKPFRFRFIVPAHNESAGITETVRSLLAVDYPKEWFDVCVVADNCRDDTAEKARAAGA
                     NVLVRNDTKLRGKGYALEYAFERLEPEIDAAVVIDADTLVSKNILSAFAARLAIGAQA
                     VQADYAVRNPEASWRTRLVAIAFGSFHVVRSRGRERLGLSCGLRGNGMCFARDLLQRI
                     PHRAYSLVEDVEYGIRLGKAGQRVHYADEAHVYGEMVSNATAARSQRKRWEEGRSGIL
                     REHLAALLKGGLLERDKVKLDLAMDLLVPPLSRIVTGVGAGLAGSFVLLWVAPPAGLL
                     GLGLFGTQAALIGAYVARGWALSNTGVGGLLSLGAAPVYMLWKLTLREKRKATQSEEW
                     IRTTRESNGQSGRVSSAAAAASGSSMS"
     gene            14084..>14696
                     /locus_tag="VFQ61_27640"
     CDS             14084..>14696
                     /locus_tag="VFQ61_27640"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9958311.1"
                     /translation="MQKNAAAHAALHIPSLDGIRAFSFLIVFLSHAGLSGIVPGHFGL
                     TLFFFLSGYLITTLMRVEFDTTSNVSFKQFYLRRVLRILPPFYLVLIAATLLSLSGFL
                     GGEVTWRGFGFQAAHLTNYYIIGHGWWTGLAPGTWVYWSLAVEEHFYFVFPLLYLWLR
                     RKGLSGRGQALTLLSICALLLIWRIVLVYGLDAPKDRTYVATDT"
BASE COUNT         2681 a         4632 c         4798 g         2585 t
ORIGIN      
        1 tgccggcctc gagctctcga acgaagcgcg ccaaatcatc cacgctctcc ccgatgtgga
       61 cggtcgcgct gcggcgcgcg ttggcatcgc gccagggtac tgggtagccg agtgcgtagt
      121 cgatcttgaa tgtgccgtaa ccttgccgaa agctccgcgc cgccttgatg gcgtacggag
      181 gcacggtgct ttcgggcagc agctcgagca gaagttggcc cgggctggtg tccgcgagca
      241 cggcgtgtcg aaaactaatt tcttccccgg tttgcagttg gactgcgacg gcgcgtcgat
      301 cgcggaccac gatccgtctg gctcgggctc cgagctgcag ctgaccacca gagagctcga
      361 gcgcggtgac caaactatcg gtcaaacgct gcgccccacc gcgcgccacg ctgtagccca
      421 ccgtggtcgc cgagaaggcc aacatatacg ccatggccgc gcccgcgaag gcatccggac
      481 ccaagtcggc gtgcatcgcg agccccggta acacgcgccg tgctgcctcg ctcgagaacc
      541 agcggttgga taaccgccgc gtgctctggg tgaagcgcag acccaatcgc aacaacccgc
      601 gcaggccgag ccgcgcaaat gcgcgcaatc caggcaaggg cccgagtaac gccgccacta
      661 acgcggcttc gttgctggcg tactccgcgg cgagccgttg aaacgtgcgc gtgtccgcct
      721 cgctgccgaa gtactcgtcg ccgagtccct ccgctcgaaa tcccatggcg tcgctcccgg
      781 cgaggcgaat caggctagcg ctcgagccgt ccagcgcggg atggcagctc tcgatctcgg
      841 cgttcaacca ctcgagaccg aaacgctcca gcttgaactc acgaaaggcg gggctgagcc
      901 gtgcgagcgg gaagaagccc gccccgagat cgtggacgaa gccaggtaac gtcgcttcgt
      961 cacttccgac ggcgccgccg ggacggcgcg agttctcctc gagtaccaat acgcgcaacc
     1021 cctgcgtggc tagtttgata gccgccgaaa gcccattcgg tcctgaccca atcaccacga
     1081 tgtcgggctc acgcattgtt tcgtgttcga tcttacattg aatcgcacgt ttccacgtcc
     1141 aacaactcga cgggcgggac tgccagcgtc gcgcggagct tgtgccacat gcggcgccct
     1201 tgaccgagtg tcgccgttgg agccaagctg aggtcaaggt gattgtcggc gttcccaaag
     1261 agaccaaaca acacgaatat cgaacggccc ttctgcccgt gggtgtggaa gagctgaaac
     1321 gcgctgggca tcgcgtcctg gtacagcgcg gtgctggact gggcagcggc gtgcgcgacg
     1381 cggaatacga gaagctcggg gccgagctga tcgacggccc cgaaggggtg ttcgcgggcg
     1441 ccgatctgat cgtcaaagta aaagagccgc agcccgcgga aatcccgatg cttcgcgcgg
     1501 ggcagctggt gttcacgtac ttccacttcg ccgcgagcga gagcctgacg cgcggagtgt
     1561 tggcttcggg cgcgacggcc gtcgcctacg aaacgctcca agactcgcgt gggagcttgt
     1621 gcctgctcac cccaatgagt gaagtggcgg gccgcatgag catccagcag ggcgcgaagt
     1681 acctggaacg gccacaggaa ggtcgcggca ttctgctcgg cggtgtgccg ggcgtggagc
     1741 ccgcgcacat caccgtgatc ggaggcggcg tggtcggcac gaacgcggcg aagatcgcgg
     1801 cgggattcgg ggcggcggtc tacgtcttgg ataccaactt ggaccgccta cgctacttgg
     1861 acgacatcat gccgcccaat gtgacgacgc tgttcagcga tcgccacacg ctgctcgatc
     1921 aactcaccaa agccgatctc gtgatcggag cggtcctgag ccgcggagct cgcacgccgc
     1981 ggctcgtgcg gcgcgaggat ctggctcaga tgaagcaggg agcggtggtg gtcgacgtgt
     2041 ccgtggatca gggggggtgc ttcgagacca cgcgccccac cagccacgcc gacccgacgt
     2101 acgtcgtgga tggcgtcgtc cactacgcgg tcacgaatat gcccggcgcc gtgggtcgaa
     2161 ccagtacgta cgcgctgtgc aacgcgacct tgccgtacgt actcaagctc gctcgtttgg
     2221 gtttggaaga agccgtgcgt gaagatcccg gcttcgagag cgcggtcaat attcatcgtg
     2281 gtgaggtcac gcatccagcc gtcgcagaga ctttcggctt gccctaccga gctctgttca
     2341 cctgactagc aaccaggagc cacgagcgct tcggcttcat gggcgccgct tcgagtgcgc
     2401 gacgcggtcg cgcgtggcgc cgcgcacgaa gcgctcgggg ttgcccagga actcgaagtg
     2461 catggtgtcg cgcagctggg gatcccgtcc cagccagtag aagccataac gctcgaaggc
     2521 gttgatccag cacgccggaa gctccgtctc cttgaaggca tcgccattgc ccgtacggca
     2581 agcccgatgt tgtggccacg ggtctacgca accgcagcag gggttgcgat caggatcgat
     2641 gtcgacggcg atcccgaaca gatgattgga gacctcaccg cctcgatatt tgttgatgtc
     2701 gcgaaagcca cccagagcct tgggctcgta gacttccttg cggctcgtgc aggtattgcg
     2761 aatgcgcttc tcgacgcact ccaaggccgg cctgattgcc agatgcacgc tgacgggcag
     2821 gccaaagaag cgcaccgtgg tggcttgaga gcgggccggc gtcgagttca gctccgcgaa
     2881 gccgactccg tctacacgcc cgtagtgctc cactcggtac tgcaacgcgc gatcggcggc
     2941 cttcccgtcc agtcgacctt tcttgacgaa cgacgggcgc ttcaaaaagt cgagggcttt
     3001 ctgcggccgg cactcgctct cccgaggcgc cttacgggcc tcggagaggc ccgggagagc
     3061 aagcatcagg aacgagaaac aggccgctga cgcgatcgcg ggcagccaag cgtgccgaac
     3121 cggaaacatg agcggcggaa gttagcaccc ccgccacgct cttgcgccac gaccgatccg
     3181 ggacgtcaca accagcctga aaagttgccg tggagccccg gatttgttca tccgagctgt
     3241 tcacgcgggt tggaccgcac cggcggcctg cagtaggaga tgacgggctt tgagtcggat
     3301 gtggtttcga agtgtgctca gtggcgagca ttttcccggt tgagggctct cgatggttgt
     3361 cgtgcgacat gagacgcggg ctgggttttg gcactctaat agacggatct ttcagcagct
     3421 tttcgcgctg gcggtgctgg gaatcgtggg gctgccgggc acgagctgcg cgagccacgc
     3481 cgagtgcact ccgccgattc catcgggggt gacttaccgt gtgcgggtcg cgccggtgct
     3541 cccgcagggc caagctgcgg gctgccacgt gatcgatctc acgaagagca tcacgccgaa
     3601 ccagttcgag atcacgacgg gccgagccgc tcccacccct tcgcgccccg actgttcatt
     3661 gaccccgctg caagacccgc ccgtccagag cagcgtgaag atcacgcgtt gcgtgccgta
     3721 cgaagccgag atgctcggct tgtcgtgtga catcgagtac gcgcccatgt gcccgggacg
     3781 catgaacttc ttcttccgtc cagtcgccgg cgagacggtc aattgggacg cgccgaccat
     3841 cagcaatctc gagttcgtga tgagtgaccg tacccagggt tgcttaatcg gcactcagaa
     3901 ttgcgacgac gtgtactacg cgcagctcga gcgcatcgtg aactaaggcc gcctgctcca
     3961 gcttcgaacg agctcgtagg cagaggtgcg cgctcatcta ggcgcgtgcc ggggaattag
     4021 cgttctggaa agggcttcga aacacgaggc agtcgaggct ctcccgacaa tccagaacgc
     4081 ccgggccgcg tctcgtgagc gctcggccaa ggagcagcgc gatggtctcc gccgtgtttc
     4141 ctctgcatat cgccccgcct cgaggcggtg gcgagagcga tcggacgtcc gagacgtcgg
     4201 ccctagccat cactcgagac gacgttcgca ttcgagatct cggtcgctgc ctgctcccgt
     4261 caccggtagc gcgtcacctg ggcgaccacg cgctgcactt cgtgggcgaa gccgacaagg
     4321 tcttggtcaa cgatactctc tctcatctgg aggcctatcg gggcgagctg gatacgctgc
     4381 ccgcgttcga actcgcgggc ccgcgcaaca agatcttctt cgatccgcgc gcgacgcggg
     4441 tggggatcgt gacgtgcggt ggcctgtgtc caggtttgaa caacgtgatt cgaggcctgg
     4501 tgctggagct ctcgcgcagc tacggcgtga agcgcatcct cggatttcgc ttcggctatg
     4561 cgggcatctt gtcgcgcttc ggcgaggcgc cggtgccgct cacgccggag tcggtggcac
     4621 acatccacca tcaaggagga acgatcttgg gctcctcacg cggagcgcag gatccggtgg
     4681 aggtcgtgga caacctggag gcgctcggca tcgatctgct cttcgtaatt ggcggagacg
     4741 gcacgattcg cggggccatg cagctggctc gtgagatcga gcggcgtcgc gcgcgcatcg
     4801 cggtcgttgg tgtgccgaag accatcgaca acgacattca tttcatcgac cgcagcttcg
     4861 gcttcgagag cgcctacgct gcctccgtcg aggtcatccg cgccgcctgc gtggaggcgc
     4921 gtggcgcggc ccgaggtatc gggctggtca agctgatggg gcgacactcg ggtttcgtcg
     4981 cctgccatgc ggcgctcgct tcgaccgacg tcgatttggt gctgatcccc gaagtccccg
     5041 ccgtgttgga gggagaacgc ggcgtgctcg cgcagctcga gaagctgctc gaagccagcg
     5101 ggcacgccgt cgtggtagtg gccgagggcg ccgcgcagga gctgatcgaa tcggacagcc
     5161 tggcgcgcag tcacgatgaa agcggcaatc agaagctgaa ggacgtcggc atctttctcc
     5221 gcgatcgcat cacccaacac ttcgcgacca agagccgccc cgtcacgctc aagtacatcg
     5281 atccgagcta ctccatccgc agcgtacccg cgtcgccttc ggacagcgtg tactgctgga
     5341 acatggctcg caacgcggtg catgcgggca tggcgggcaa taccgaaatg ttgatcggac
     5401 gttggcacgg ccgcttcgtg cacgtaccga tggagctcgc cacgcgcaca cgcaagcagg
     5461 tggtaccggg agacgagctg tggtgcagcg tcatcgagtc caccgggcag ccgcgcgctt
     5521 tcgtctagca ccggccgccc gcgggcgtcg caaccggttc tcagaataga gtggtgtcgt
     5581 agcgcagtgc cgcgtacagc gcataggcaa aggagatgtc cggcccgttc gccacggttt
     5641 ggcgcaagcc acgcaggccc gcttcgaaca ccaaaccatc gacgatcgtg tagtacatgc
     5701 cggcggcgcc agtgtagttg ctcaccgaac ctggttcgga tggcgtcaac gacaacgtcg
     5761 cgcccgcgtt caagaacagc gtcgtgcggt gcttcaccca gcccagaccg ccaaacatca
     5821 ggaagcgcgg atcgatccgc acatcgatgt tcttggtcgt gggcgtgtag gtgagggcgg
     5881 ggctgtaacc gacgccggca gaagccgtga gttgtcctcc ggcgaagcgc gtcgtgtagc
     5941 ttacgcccac gagcgcattc gagccgaagg tcggatagat ggtgtccgtc caaactccgt
     6001 cgcgtcgttc gcgcacgtag ctcacgccgg cgctcaggcg caccaggatg ctttcactga
     6061 attgatgggc gtagccttgc tccaagctca agcgccacag ccgcgtgcca ttggaaacgg
     6121 agctgtaagt gcccgtagtc gccgtaaaca gttggtcctt cgcggtgagg cgcaggtcat
     6181 atcgcaagcg aagatccggc cctgactgga gcggcagctc acgacgtgag tctccgacgc
     6241 cgccgatcac gaagtagccc gcaccgaagg tgaacgagct cgttcgggtc agcgcgtgcg
     6301 tagccgtgaa atcggttcga agaaccgcga cggtcagtgc gtcccgcacc gccgcgctgt
     6361 tcggattggt ggccccgatt ggggtacccg ttgcagtacc cggcacggcg cccggccccg
     6421 tcgtggtgcc cggacccagc ggcgtgccgt tgccagtcgt ggtaccgggg gtgccggtcg
     6481 gagtcgtgac cgtgccgccg ggaccggttc cgggcgtagc gggaaatgtg cccgcgggta
     6541 aacccggcgt tccggagagg gcggtactgg caaagttctg ccgaccatac gacgcagttt
     6601 gcgtgatttg gtacgcggta cgacgctgac gaaaatcgaa accgatgcgc gccgtgtgca
     6661 gcacgtaaag ttccggcttc ttctcatcca gcggcgagag cacgaacaac ggtgaataac
     6721 caaagctcaa cgccgaccgg cgcccggacc aacgcaggtc cgcgtagggc cgcgtctcgg
     6781 cttcgggcac acggtcgttc gtttccacac gcagccgcgc ttccgtacga tccccaaccg
     6841 agagcgagag cgcttgcgcc aaaatcggca caatccacgg ggacatgcct caacctcgct
     6901 cgacccgagc cgccaaactc gaggtcctca cacccaaatc gagggactgc gaagcggcgc
     6961 gagtcatagg caccacggtg caaggcgtag aaaaaacggc gcagagcgct ggcaagcaga
     7021 gcgtaggcaa cacggcgcga agcataggca acacggcgca gagcgtagcg actttcctga
     7081 accgagcgta gggagtttgc tgcctgatgt agcgaatgga acacttgcct agggattcgc
     7141 gcgaggattc gcgcattcac cacgaaaagc cggcatgtgc tggaacgatt cagtcgcaaa
     7201 tgagcagggg ggccggccca gcgccgcgtc tgcatgggtc cgtgacgttc ggcgcttaaa
     7261 gacccagcgc aagaaggcgc agtgccgggt gcatggctgg tgcagcgccg ggtgcacagc
     7321 gccgggtgca cagcgccacc cgatagagct cggttgcggc gcgctcagcg tgcaaaaaaa
     7381 cctgtgggct tgagcgaggc tgacggctag gctacctgct tgtttctggc cccgacatcg
     7441 ctgccgggcg cagtaaaact ggaatctcgc ctcgaccggg acgtgccggc gaagcggttg
     7501 ttcagtttgc agcgattgct cagttaggaa cgtagtctcg tggaaaagac gacagcgctt
     7561 gcccgctccg aggacaatct agcgatcagc gatctctggc gcgaggccga gtggcagcgc
     7621 ctgtggttgg ctgtgcaagc gcagcaatgg tcctcgctcg cgctaattcc cgcagcgccc
     7681 ggcgctcccg aggattttgc cctgacgatc gcggtgacgt tggcgaagac gggcatcgtc
     7741 catttgggag ttccgatcca cgttgctgac ggcgcgcgga ttcagatggc cgatttggcg
     7801 acgttcggcc aagaagtcgc gcgctgcaaa gagtacggag atcgagtgtt gatggcgctg
     7861 gcacccatcg atgagaaccc ggtgtcggtt acattggcga aatcggcaga ttttgcgctg
     7921 ctctgcatct tgctcgaaga tatggactcg tcccaatcga agaagacggt cgcggcgatc
     7981 ggacagaatc ggttcattgg ttcggtgatg tttcatccga gccagctcaa cggagctgcg
     8041 cgccgctgag ctagtcgggg cctgcgagcg tgtttgagaa agtctccggc gtgcgttgct
     8101 agagcgtgag ctcgagcacc tcgaggctga gggggctcag gtcgagcggc aaggttcgac
     8161 cgcgtagcat gctggcgtct gcggcctcga gccgcggcac gttcgagcgc aacacgaagc
     8221 ggcgcgcggc gaggacctcg ccgcactcgc tcaagtcgag cgacatctca gcccgatggg
     8281 tggcgtcgcg ctggatcaaa accagcacca acgttcgtga ctccgcgttt cgcgaggcaa
     8341 acagcgagat ccgctcggtc gcttgcgtcg gaaccgacca atcgaggaag cgagccccag
     8401 ccccgtcgaa gtcccggaaa gcccggaatg cttcgaaggt gaagctgccg ctccgcggac
     8461 cgcgccaata gaaggcccag tccagctcgt gctgaccgaa gcggcccaag gcctcggcgg
     8521 tcgcgagtgc gccgctgata tggtcctcgg cgccaaaact ccattcgccg agcgaaagcg
     8581 cccggcctgg gtagtaccgt tcgacccact ggcgcaaccg cggaatcagc tggattggag
     8641 cctcaatcca ggaatcgtcg acgtaactcg ggtcccacag tgcgcgcgta ctgcggagcc
     8701 gcagcgctgc ggttgcggca tccgtgcggc tgttcgcgta aaccccgtct gcttgggggt
     8761 aaaaatgcac gtcgagcacg tccaatagcc gggtgcccgt acgccgctcg tgggccgcga
     8821 gcgcttggag ataatacgcg agcagcggca ggcctccgtg gcgtcgtcga tcgagacgca
     8881 gccccaagcg actcgtcgtg tcgcggggag agtcgaagta gcccacgtag ccccaagaag
     8941 ctggccccgc gatcagcgcg tctggatctg cggcacggat ttgcgtcgcg taagcgatcg
     9001 tacgctcgat gagctcgtcg taactcaacg gctcggggtg cacgtcgcgg tgggtcacgt
     9061 gccagagatc gggctcgtta tcgaggatgt acatgtgcac ccggcgcacc ccgcgcgctc
     9121 gatccgccgc gcggagagcg cggatccagg cgccgatctt ctccggaggc gcggcgatgc
     9181 tcgtgcgtga agcagggccg ggcgcgatgg gttcgccgtc gccgcgcaag ccattgccgg
     9241 catcggggcg gttcggatcg gtgtgctgtt gagggccgaa caccgagacg gggaaagaaa
     9301 acgagctccc gtccttcgcg acccagccga tcatcggcac ggtgagtgcc atcgacaggc
     9361 cgcgtgacag gccatcctcc agccaatcgt cgagcgcgct gtcacctctc acgttctcga
     9421 agaaccaatc gctgcccgcg ttccaagtgt ccccgagctc gaagttcagc cgggtcatcg
     9481 ggttgccgcc aatgcgacga gcgctctcgt gcacgtaatc gccggcggca aaaccataga
     9541 ttccagggtg aatcgggcgc gcggggagcg agcagcgcac cttcagtacg tctggtcgac
     9601 ggctcgagac tacgcgcggc gaagccgcgg cccggaccgg ctcgtcccgc aacggaaagc
     9661 acagccagcc cgcgctcgcg acgagcgcca gcccgccgac taggcccgtg aggagctgga
     9721 tcagagctcg ccggtgccgc ctcacgggct taccgtcggt agccaggatc ggccagcgcc
     9781 ctcaagagct cgacgcgcgc gcctcgaagc cttcgcggac ggcggccgcg atcgccgtca
     9841 cgaactgatc gagcccggcg tgctcttcga cgaacttgcg cgcggcctgg ccgatgcgct
     9901 gcgcctcgtc cgggttgcgc agcacgcgtt ggatttgcgt ccgcagcgcc accggatcgc
     9961 tcggtggcac gtagatgccc gtctgcccgt ccacgatcgt gtccgtctgg ccttgcgtgc
    10021 gcgttgcgat gacgcacttg cccatggcca tcatctccaa gatcgtagcg atgccggtct
    10081 ggtagtggtt ctccaagatg gggaccacgg ctaccgcggc gcgttcgtag aggtcccgca
    10141 aatcgaagcg gttgagcttg ccccaggaaa cattccccgg cagcggcttt tgcggctcga
    10201 gcttgcgttc gatccagggg cttcccgccg cgatctgcac acgaaccggt aggtcccgca
    10261 cggcctctac caagcaatcg tagtcacgca gcagctgacc cgcggcgcaa atcaaatccg
    10321 gttcgaccgg cctctccacg ggccggaaaa actgctggtc ggcgtggtag tggatgcgct
    10381 tcaccttcat ccgcggcacg ccgagttcac ccgtgaccaa ctgctcctcg ctggtcgaat
    10441 agaccaggat gcgatccagg cgcgtcgccg cgttcaggta ctgaaagaag acccgcttct
    10501 ttcgtgggaa cagcgtatgc gcgatcatcg tgtgcgagca gcgcgcggag cttcgcgcca
    10561 acaacgcgca cagcggcagg ccaatgtcct cgccgctggt caggatggcg tcgtagttct
    10621 tgcgttcgag gaacccgagg taagcgaccg cggcggagta gcccaaggat ttctgcgtca
    10681 gcttcacgcg cttctggttc gagcgttcga cgtccagaaa atcgatcacg tcggcgcgca
    10741 gcacgttcga cagttcgaag acgtcgagcc tcggctctcg gcccttcgcg atgtctcgtc
    10801 gcagctgctc accaaaccga cctgcagcca gaatcagcgt tctcatggaa ttgtcgtttc
    10861 agccttctaa cccagatttc gtgtcggcga ccaacgaagt ggtctactcc gggcgcgagc
    10921 ggtgattcgc tcgtcgcaaa aactcacacc gatcgcgggg accggtggcc tggagagccc
    10981 gactggaacc agtgggtgga atcaaccggc cgcgcccaat ccaagttacg ggtcgagatc
    11041 cccagccgag atatcgcgcc ggcggatccg aacgcccgtt cagcaagtaa cgaggttgcg
    11101 ctgcactaac cgagttgcac tccgtgggtg gcgctgatta cctttcatcc tgctggcgtc
    11161 tcgcgccgcg agccttggcg ctccgcgacc cctaccctcc ccccacgcat cgatgcgcga
    11221 tgcgcgcgac gagcaaattg agcccgagac cagcaggcac agtcggagaa gcatggctac
    11281 tgaacgtgac gaagacgacg aagagggcgg aggctccgcc cagcccgatc tcattcagag
    11341 ctacctgtct ttcgccgccc gcgcgattcg cagccgcatt ccactcgtgc tcggcacgat
    11401 cgccgtggtg tctttgctga cggtcgccgc ggctgcggtg tggccgcgca cctattcgtc
    11461 gctgactcag atcatggtcc agagtcagaa catcccgggg gttcaaaagg atgacccgtt
    11521 caccggcgcc gatcgcgtga tcatgcggcg ggagaacatc gaggcgctcg tcaagcagac
    11581 cgagctgacg aaacactggc aaagagggcg ctcgccggcc ctgttactca aagatcggtt
    11641 gatggagttg atccgcggcc ccatcccgga gagcgcgctc gcgcccgtct tggtgagcac
    11701 cctcgagtca cgtatctatc ccgacgcgag cgatcgaacg ctcaccatca cggtagactg
    11761 gagtgacccc gagctggccg caaagatcgc cgaaacggcg atgaaacgct tcgtcgactt
    11821 ccgtcagacc gccgagatcg ctagtcgcca aaaggagatc gatctgctcg aggcccacgc
    11881 ggccgacacg cgcaagcggc tgaaagagcg cgcggacgaa ctgaagaagc gccgtgagga
    11941 acggcttgcc aaccgcgagc ccgatcccgt cccggtgccg ggggaagccc cgaagccgaa
    12001 ggtggtccgt acccggcctc aacgcgatct ggcggcggag ggggagcttg ccactctgcg
    12061 ggcggatttg accgcgcgcc gcggccaaca gatggaacgc gagcgccagg atcaaagtca
    12121 ggtcaacgag ctccgctcga aattggacga gaccttgcgc gtggtgaccc gctcgcatcc
    12181 tgacgcggtg atcgcgcaac gccgtttgga cgcggcgatg gccgcggccg ccgcaaacaa
    12241 gagccaaggc acgcaggacc tcgcggacct ggagcggcgg gtcaaagaag cagaaggcag
    12301 agtcgtggcg ggcggcggat ccatcgccgt tatcggagac tccggtagcg ctacgccttc
    12361 cgtgcccccg gaaattcgac gcttgatcga ggacagctcc gaagaaggcg tcgacccaac
    12421 actcatctcc gaggtcgaac accaggcggc caagctgacc gatctccggg atcaaatcga
    12481 caaagagtcc ctcaacctgc agcgcgcgca gcaagccttc gagacccgct accaggtcgt
    12541 gatccccgcc gaaattcctg gcaaaccgaa gaagcccaac gtgcgcatga tcatcgccgg
    12601 tggcgtgatc ggatcgatcg tgctcggttt ggtgttggca attctggccc agctacgcaa
    12661 gggcaagatc gtagccaagt ggcaggtcta caacctcgga gtgccggtgc tggccgaaat
    12721 caagttccct cgcggtccga acgcctgaag gtgcatacgt aatgactcta tggtctgggt
    12781 tttctgcgtt gtcgggcttg gtcggtgcgc tggcggtccc cactggcttg ctcacgggtt
    12841 acttgggagc gctgacggcg ctctcggccc cgatcgagcc gccaaaagcc agtgacaagc
    12901 cgtttcggtt ccggttcatc gtgccggcgc acaacgagag cgcgggcatc acggagacgg
    12961 ttcgaagcct gctcgccgtc gattacccca aggagtggtt cgacgtgtgc gtcgtcgcgg
    13021 acaattgccg cgacgacacc gcggaaaaag cgcgtgcggc gggggccaac gtgctcgtgc
    13081 gcaatgacac gaagctacgc ggcaagggct acgcgctcga gtacgccttc gagcggctgg
    13141 aacccgagat cgacgcggcc gtcgtgatcg acgccgatac cttggtgtcg aagaacatcc
    13201 tcagcgcgtt cgccgcgcgc ctcgcgattg gggctcaggc cgttcaagca gactacgcgg
    13261 ttcgcaatcc cgaggcctct tggcgcacgc ggctggttgc gatcgcgttc ggaagtttcc
    13321 acgtcgtgcg ttcgcggggc cgtgagcggc tcggtctgtc ttgtggcttg cgcggcaacg
    13381 gtatgtgctt cgcgcgagac ttgctccagc gcatccctca tcgtgcctac tcgttggtcg
    13441 aggacgtcga gtacggcatt cgcttgggga aggccgggca gcgcgtgcac tacgcagacg
    13501 aggcgcacgt gtacggggaa atggtctcca acgcgacggc ggcgcgttct cagcgcaagc
    13561 gctgggagga aggccgaagc ggaattttgc gcgagcactt ggcggctttg ttgaagggag
    13621 gcctgctcga acgcgacaag gtcaagctcg atttggcgat ggacctgctg gttcccccgc
    13681 tgtcgcgcat cgtgacgggc gtgggcgcag gattggccgg atctttcgtt ttgctctggg
    13741 tcgcgccgcc cgcgggcctg ctcggcctgg gattgttcgg gacgcaggca gcgttgatcg
    13801 gtgcgtacgt cgcgcgcggc tgggcgctgt ccaacaccgg agtgggcggg ctcttgagct
    13861 tgggcgctgc cccggtgtac atgctctgga agttgacgct gcgtgaaaag cgcaaagcga
    13921 ctcaatccga ggagtggatc cgaaccacgc gcgagagcaa tgggcagtct ggcagagtca
    13981 gcagcgccgc cgcggcggcc tcgggtagtt ccatgagcta gcgcggagag cggattcgca
    14041 gagcctcatt cgcagagctg gcgccccctc acttcgattc tccatgcaaa agaacgcggc
    14101 agcgcacgcg gccctccata tcccgtccct ggacggcatt cgtgccttct ccttcctgat
    14161 cgtgttcctg tcgcacgcag gtttgagcgg catcgtgccg ggtcatttcg gtctaaccct
    14221 gttcttcttc ttgagcggct acttgatcac cacgctcatg cgcgtggagt tcgacacgac
    14281 atcgaacgtt agtttcaagc agttctactt gcggcgtgta ctccgcatcc tgccgccgtt
    14341 ctacctcgtg ctgattgcgg ccacgctgct gtcgctctcg ggcttcctgg gtggtgaggt
    14401 cacgtggcgc ggcttcggtt ttcaagccgc ccacctcacg aattactaca tcatcggtca
    14461 cggctggtgg acggggctcg ccccaggcac ctgggtatat tggtcactgg cggtggagga
    14521 acacttctat ttcgtgttcc cgctgctgta cctgtggttg cgacgcaaag ggctgtccgg
    14581 gcgtgggcag gcgctcacgc tgttgtcgat ctgcgcgctg ctgttgatct ggaggatcgt
    14641 gctcgtatac gggctcgatg cgccgaagga tcgtacatac gtcgcgaccg acacgc
//
LOCUS       DASTKU010000788         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1717793, whole genome shotgun sequence.
ACCESSION   DASTKU010000788 DASTKU010000000
VERSION     DASTKU010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717793"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFQ61_27645"
     CDS             <1..334
                     /locus_tag="VFQ61_27645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958312.1"
                     /translation="CPCQAGPDLNHDLILLFPFNLARANSLPHRYTLESEDGTLSRER
                     ALARDARANEQQRAELTFEHLPEAHAYRLSCDDGTGSYTLFEASTLSDLQAQFGNRAD
                     MATAIEDT"
     gene            complement(450..>2136)
                     /locus_tag="VFQ61_27650"
     CDS             complement(450..>2136)
                     /locus_tag="VFQ61_27650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958313.1"
                     /translation="LLLTNQLNGYGTSQVRYSDSHPSTPVNVPGSDRLVASNSNFLVK
                     YPATGTYTLSVNWNQKTFSVSNQLTAEQRLATCAQDPRVVAGLVSAEVCAGADIFFRE
                     TFGGNGRTCGSCHPMDNNTTLDVKFITQLHQRNPKDPLFVNENDPNLANLETTDLVNF
                     ASILENVDGFQSPTQRFVSRTVSHVLSLKTSIARDPGDNTSSPPLERLGWGGDGVGDG
                     SLLAFLEGAITQHFTKDLRRRPNVDFRTPTAREAELTRAFQLSLGRLNELNLQQVTLV
                     DPDAEEGRHAFMDPQRGRCNVCHSNAGANFIDTGLNRNFDNGIRFASNTNFITRGSLD
                     GVLLSDGGFGGQDLAQPNLDTDEDGVNEGFGNGTFSPPPLIEAADTGPFFHNAFHFLS
                     RLPDDIDEAVNFYRLPGDPFGKSLGGKFLEQRFGTPLVLDGVDSANIARFLRVLNGAF
                     NLDLAKQRLDAANSLASRFGDTRADIQKRLMELAVAELDDALEVLPDNGDVLPAARTS
                     VQQAKDEIALGLTATTASARRTRITNALARVASGRTQFGSNLTFQLGEGNLMY"
BASE COUNT          391 a          625 c          703 g          417 t
ORIGIN      
        1 ttgcccgtgc caggcggggc ccgacctcaa tcacgacttg attttgctct tccccttcaa
       61 tttggcgcga gcgaactcgt tgcctcatcg ttacacgctc gagagcgaag acggaactct
      121 gtctcgcgaa cgcgcgctcg cgcgggacgc gcgtgccaac gagcagcagc gggcggagct
      181 cacgttcgaa catctgcccg aggcgcacgc atacagactt tcgtgcgatg acggcacggg
      241 ctcgtacacc cttttcgaag cgtcgacgct atccgacctc caagcgcagt ttggcaaccg
      301 agcagatatg gccacagcca tagaagacac ttgaaatgtc caccgacgtc gcgcgggcgg
      361 gtcgaaacga cgataatctt cgtctgccta cgagagtcgg actgagcgct gccgggatgc
      421 agcgcccaat ccgtgacgtg cgacccaact cagtacatca agttgccctc accgagctgg
      481 aaggtgaggt tcgagccgaa ttgggtgcgt ccgctcgcga cgcgagccag tgcgttggtg
      541 atgcgtgtgc gacgcgcgga ggcggtcgtg gcggtcaacc ccagcgcgat ctcgtctttg
      601 gcctgctgca cgctggtgcg ggcggcgggg agaacgtcgc cgttgtcggg cagcacctcg
      661 agcgcgtcat ccaattcggc gacggcgagt tccatgagtc gcttctgaat gtcggctcga
      721 gtatctccga atcgggaggc gagcgagttg gccgcgtcaa ggcgttgctt ggccagatcg
      781 agattgaacg cgccgtttag gacccgcagg aagcgggcga tgttggcgga gtccacaccg
      841 tcgagcacga gcggcgtccc gaagcgctgc tcgaggaatt tgccaccgag cgacttgccg
      901 aatgggtctc ccggtaagcg gtagaagttg acggcctcgt cgatatcatc cggtagccgg
      961 ctcaggaagt ggaacgcatt gtggaagaag gggcccgtat cggcggcttc gatcaacggc
     1021 ggcgggctga acgtgccgtt gccgaagccc tcgttgacgc cgtcttcatc cgtatcgagg
     1081 ttgggttggg cgaggtcctg tccgccgaag ccgccgtcag aaagcagcac gccatcgagc
     1141 gacccgcgcg tgatgaagtt cgtgttgctc gcgaatcgaa ttccgttgtc gaagtttcga
     1201 ttcaggcccg tatcgatgaa gttcgctccc gcgttggagt gacacacgtt gcagcggccg
     1261 cgctgcggat ccatgaacgc gtggcgaccc tcttctgcat ccggatcgac gagggtcacc
     1321 tgctgtaggt tcagctcgtt gaggcgaccc aagctgagtt ggaaggcgcg cgtgagttcg
     1381 gcttctcgcg cggtcggggt gcgaaagtcg acgtttggac ggcgccgtag atccttcgtg
     1441 aagtgctgcg tgatcgcgcc ttccaggaat gccagcagcg agccgtcgcc cacgccgtcg
     1501 ccgccccagc ccaagcgctc gagtggcggg ctcgaggtgt tgtcgcccgg gtcgcgcgcg
     1561 atgctcgtct tcaatgacaa gacgtgggac accgtacgcg agacgaagcg ttgggttggg
     1621 ctctggaagc cgtccacgtt ctcgaggatg gaggcgaagt ttacgagatc cgtggtctcc
     1681 aagttggcta gattcggatc gttttcgttg acgaacaaag gatccttggg attgcgctgg
     1741 tgtagctgcg tgatgaactt cacgtccaga gtcgtgttgt tgtccatcgg atgacagctg
     1801 ccgcaagttc ggccgttgcc cccgaaggtc tcccggaaaa agatgtcggc gccggcacac
     1861 acctcggcgc tcaccaggcc tgccaccacg cgcggatcct gggcgcaggt ggcgagccgc
     1921 tgctcagccg tcagctggtt cgagaccgag aaggtcttct gattccagtt gaccgacagc
     1981 gtataagttc ccgtagccgg gtacttcacc agaaagttcg agttggaggc gaccaagcgg
     2041 tcgcttcccg gcacattgac cggcgtcgat gggtgggagt ccgagtagcg gacttgcgac
     2101 gtcccgtaac cattgagctg attcgtcaaa agcaga
//
LOCUS       DASTKU010000789         8081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_600669, whole genome shotgun sequence.
ACCESSION   DASTKU010000789 DASTKU010000000
VERSION     DASTKU010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600669"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="VFQ61_27655"
     CDS             <1..903
                     /locus_tag="VFQ61_27655"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET9958314.1"
                     /translation="WLARPLVWLLSSSANVLLRPFGDKTTFTETRHSAEELQQLVEEA
                     TQAGTIHPEAGEIAARALELPELTVADVMVPRRDVVMMARQAKADDIRRIVLEHTHSR
                     LPVYEERVDNVVGYISVKDLIALAWDHQLIVLEDVLRPAFFVPESKRAVELLKEMRTR
                     HLPFAIVVDEHGGMSGIITMEDLLEELVGEIFSEHEPETPELIQRQPDGSFVVAGSTP
                     VRDINRGLALDLPDEGPWTTIAGLCLALAGRMPQAGECFEVPKRITLEVLDASDRRIR
                     SVRIHHRAEPEPTDTEAVPPELEA"
     gene            1153..2856
                     /locus_tag="VFQ61_27660"
     CDS             1153..2856
                     /locus_tag="VFQ61_27660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958315.1"
                     /translation="MRTIRLVSALLLIVLACSNGEISPAPGAAGVGAEPSTGGRPPFG
                     QGGAPSNGAGSTAVGARGGGVSGGHSGGLGSTGVSAGNSGTNTGPSAGTGPGGVTGQL
                     SGGGQGNTGGRPPQGGNGNPTGGANDGGSGAAPHAGSTSFNGGTGGSITGACPGNCRL
                     PAAGACTSPQVRITEVDLGSPVQSNEDETGLLPLMLAAKPGGGSRLAWMSGSARNASS
                     QNSTVHVADLDCDDHLVGTPFTLAAHDFQDIAADADGGVVMLTRDGDGSTADFCGDVN
                     NLCKLPTDRPACYNMFMVRFDCSGEEQWATKLTTSSPSALPYTSGGGQNHMIWWYQHH
                     GRLASDGTNYAAYFCDAITVTNNTCSDKVDIHEGDRMQVVGPNGAVLTGHDSFGLGCS
                     HSWNTRLVWDPSTKHFVMVCATDNVGRVALPAPYRTLYTAKTLSTLSVGDVVLATGGG
                     YWVTACDESQVHLLKFDDITNNGVKVSSDTVIGSAAFSHLVSYGSEHMVVSWPSGNAI
                     SAQVLSAADGSKLGEPFTISVPNNRYTSFKSFPDGSVAYAAPGTSNTKIRIARVLPCS
                     G"
     gene            2951..4132
                     /locus_tag="VFQ61_27665"
     CDS             2951..4132
                     /locus_tag="VFQ61_27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9958316.1"
                     /translation="MNVQRLLGTGFRRAATCALLTTLSGTGCGKKSSDAIASSASSSA
                     AKNSAAADKGSALTVGFIYVGPKDDYGYNQAHAEGAKAVAALPGVNVREEEKVPETVD
                     VQKTMESMINLDGAKLLFPTSFGYFEPHILKLAEKYPQVTFLHCGGLYQEGKHPKNAG
                     SYFGYIDEAEYVAGITAGLTTKTNKLGFVAAKPIPQVLRNINAYTLGARSVNPQAVLT
                     VVFTGDWSLPVKEAEAANSLIDQGADVLTAHVDSPKVVIETAERRNVYSTGYHASQAA
                     LAPKGYLTGAEWNWQVIYKKYVEAVQKGETPEHLVRGGFKEGYIKMSAYGPAVNDAAR
                     AKADEAKAKLTAGDFTIFKGPLKDNSGKELLAAGSTQIQTDINLEKMNYLVEGVKGAL
                     N"
     gene            4145..5278
                     /locus_tag="VFQ61_27670"
     CDS             4145..5278
                     /locus_tag="VFQ61_27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9958317.1"
                     /translation="MSSAASSSSSETLIADRESSPHALRWLLIRGLSSLEELLVPLLG
                     FMVSMSLFGVFIALVGKPPLEVFAQMYRGAFGTTFSFQNTLMRAAPLMLTGLCTALPA
                     RLGLIVIGGEGALVVGGVTAAAVAPALSSASSVTIQLAMALAATIMGGLWIAAAGWLK
                     VSRGVNETISSLLLSYLGIALMNHLVEGPLKDPASLNKPSTFPIGEANMLPNLPGMDL
                     HYGLLLGVLACLLCSLLMDQSRFGFASRVVGGNVRAARLAGLPVARLVVLACFLGGAA
                     AGLAGMIEVAAVHGKANGSLICGYGYTGILVAFLARHSPLAVMPVALLLGGISASGGL
                     LQRSFGLPDASVNVLQGVLFVVLLTSESYRGKLRPFRHWTQRV"
     gene            5300..6253
                     /locus_tag="VFQ61_27675"
     CDS             5300..6253
                     /locus_tag="VFQ61_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9958318.1"
                     /translation="MNTLTTIAANPEASLGLLGVPLAVLAGALRVSTPFLFVSLGECL
                     TERSGRVNLGLEGTLVMGAMVGYGGSYLSGSPWLGVLGAAAVGAILGALHAFLCARPR
                     VNDISVGIALMLLGVGLAFFLGKPLVQPSAARLPSISLGFFSDSPQVRSALQVNALFL
                     LGLVLAPTLRWALASTRWGLVLRTVGESAEAARAMGYDVARVRLLATAAGGALAGVGG
                     SFLSLFYPGSWNEGLSSGQGLMAVALVIFARWDPVRCLYASLLFGGASALGPALQSVG
                     VTQGYYLFNAAPYALTLLIMILSSSATHASADSPAELTRVR"
     gene            6262..7923
                     /locus_tag="VFQ61_27680"
     CDS             6262..7923
                     /locus_tag="VFQ61_27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9958319.1"
                     /translation="MKPAVHSSRAVGNANTSVTSGHAAPDVSSRTSSGIRVKAAPPEL
                     EVRAMCKRFDSLIALDDVSLRLRPGSFHALLGENGAGKSTLVKCIMGFYRADSGSVLV
                     DSQPIELKSPREAHRHGIGMVYQHFTLVENMTVAENLVLSRARVPAVIDFAKERAELE
                     SFMDTMPFRVPCEKRVRQLAAGEKQKLEILKQLYLGSRIVILDEPTSVLTPQEADEVL
                     GLLRNMAHDGRISVAMITHKFREVMAFADEVTVLRRGRVVGGGSTNELTPETMAELMV
                     GSKPPETRLERAPQAPGRTILELEGITAGDDIGVQVLNGVSLRVRAGEIVGIAGVSGN
                     GQDELVEVLAGQRAASAGSVCVDGKPFRPTRAQLRARNVRLLPESPLRNACVANMSVA
                     ENIALRVFDTSPFTRLRAFVSRAAMRRNARARVNEYRIKTPSVDTPIGALSGGNVQRA
                     VLARELAGTVALLIAANPCFGLDFAAVSEIRERIVTARNSGAAVLLVSADLEEVFALA
                     DRILVMSAGRIVFETEASLADPTEIGRHMAGHSGPSDRPESLPGT"
BASE COUNT         1538 a         2471 c         2560 g         1512 t
ORIGIN      
        1 tggctggcgc gacccctggt ttggctactg agctcgagcg ccaatgtctt gcttagacca
       61 ttcggtgaca agacgacatt caccgagacc cgacattccg cggaagagct gcagcaattg
      121 gtggaggagg ccacccaggc cggaaccatt catcctgaag ccggcgaaat cgccgcccgc
      181 gcgctggagc taccagagct gaccgttgca gacgtgatgg tgccacgacg cgacgtggtc
      241 atgatggcac gccaagccaa ggcggacgat attcgaagaa tcgtgctcga acacacccac
      301 tctcgcctgc ccgtttacga ggagcgagtg gacaacgtgg tcggttacat cagcgtcaaa
      361 gatctcatcg cgttggcgtg ggaccatcaa ttgatcgtgc tcgaggacgt attgagaccc
      421 gccttcttcg tgccggagtc caagcgcgcg gtcgagctgc tgaaggaaat gcggacgcgg
      481 cacctgccat tcgcgatcgt ggtcgacgaa catggtggca tgtccggcat catcaccatg
      541 gaggacctcc tggaggagct cgtcggtgag atcttcagtg aacacgaacc cgagacaccc
      601 gaattgatac aacgccagcc cgacggctcg ttcgtcgtgg ccggctccac tcccgtgcga
      661 gacatcaatc gcggcttggc tctggattta cccgacgaag gcccgtggac gaccatcgcg
      721 ggactctgcc tagccttggc cgggcgaatg ccacaagcgg gcgagtgctt cgaagttcca
      781 aaacggatta ccttggaagt tttggacgcg tccgatcggc gaatccgttc ggtgcgcatt
      841 catcatcgag cggagccgga acccaccgac accgaggcgg tgcctccaga gctagaagcc
      901 tagcctcggc aacccagcgc tgggattcag ttgctcgccg gctgcgctgg tctcccgctg
      961 aagacctgac taccggagca ctccgagggc ccgtgctcct gggcacgaac aaccgaaggc
     1021 ggtgtgggcc atgactcaca tgcgcaagcc gctcggcacc ggtgttcacg ggacagagat
     1081 cgctgaatgg cgggatctcc gcctcttttt tggaccgtgc agagcgctgg agtcactgat
     1141 cttgcgttac tcatgcgcac cattcgactg gtttcggctc tccttctgat tgtgcttgcc
     1201 tgcagcaacg gcgagatttc gcctgccccc ggggcggccg gggtaggagc ggagccgtcc
     1261 acgggaggcc gtccgccgtt cgggcaaggt ggagctccct ccaatggtgc tggctcgact
     1321 gcagtcggcg cgaggggtgg aggcgtatcc ggcggccact cgggagggct cggctccacg
     1381 ggagtcagtg caggcaactc gggcacgaat acgggcccga gtgccggcac cggaccaggc
     1441 ggcgtcaccg gacagctctc gggcggcggc caaggcaata ccggcgggag acccccgcaa
     1501 ggaggcaacg gaaacccgac gggcggcgca aacgacggcg gatcgggcgc cgccccacac
     1561 gctggcagca ctagcttcaa cggaggtacg ggcggctcga tcactggcgc ttgcccggga
     1621 aattgccgct tgcctgcagc cggcgcatgc acgagcccgc aagtgaggat cactgaagtc
     1681 gatctcggca gtcccgtgca gagtaatgag gacgagaccg ggcttctccc gctgatgttg
     1741 gcggccaaac ccggtggtgg ctctcgtctg gcgtggatga gtggctccgc aagaaatgcc
     1801 agtagccaga acagcaccgt gcatgtggca gacctggatt gcgacgacca tctcgtcggt
     1861 actcccttca cgctcgctgc gcatgacttc caggatattg cggccgacgc ggacggcggc
     1921 gtagtcatgc tgacccgcga tggggacggg agcacggccg acttctgcgg tgacgtgaac
     1981 aacctctgca agctcccaac cgatcgccca gcttgttaca acatgttcat ggtgcggttc
     2041 gactgctccg gagaggagca atgggcgacc aagctgacga cctcgagccc cagcgcactc
     2101 ccctacacct ctggcggtgg gcaaaatcac atgatctggt ggtatcagca ccacggtcgc
     2161 ttggcctccg atggcaccaa ctacgcggcc tacttctgtg acgctatcac cgtgaccaac
     2221 aacacctgta gcgacaaagt ggatatccac gaaggcgatc ggatgcaggt ggtcgggcct
     2281 aatggagcgg tgctcaccgg tcacgacagc ttcggtttag gctgcagtca tagctggaac
     2341 actcgcttgg tatgggaccc cagtaccaag cacttcgtga tggtctgcgc gaccgacaac
     2401 gtcggacggg tggccctgcc cgcgccgtat cgtacccttt acaccgccaa gaccctatcg
     2461 accctgagtg ttggagacgt cgtgctcgca accggaggcg gttattgggt caccgcctgc
     2521 gatgagtctc aagtacatct gctcaagttc gacgacatta cgaataacgg cgtcaaggtg
     2581 tcctcggaca cggtcattgg aagcgccgca ttttctcacc tggtgagcta cgggagcgaa
     2641 catatggtgg tctcttggcc atccggcaac gcgatcagtg cgcaagtgct gagtgccgcc
     2701 gacggctcca agctcggcga gccgttcacc atttccgtcc cgaacaaccg ctacacgtcc
     2761 ttcaagtcgt tccccgacgg tagcgtcgct tacgcggctc ctggcacgag caacaccaag
     2821 atccgaattg ctcgggtcct accttgttcc ggataggtcg aaaccgggac cgcgatcccg
     2881 cgaactttgg ccgatcggaa ttgtccccga gacgcccccg aaacttgcct accgagatac
     2941 ctcggtgcgc atgaacgtac aacgattgct cggcacgggc tttcgccgag ccgcaacctg
     3001 cgccctactc accactctgt ccggcacggg ctgcggcaag aaatcatccg acgcgatcgc
     3061 gagctcggct tcgagctccg ccgccaaaaa cagcgcggcc gccgacaaag gcagcgcgct
     3121 caccgtcggc ttcatctacg tcggtcccaa agacgactac ggctacaacc aagctcacgc
     3181 ggagggcgcc aaagccgtag cggcgttgcc gggcgtcaat gtccgtgaag aggaaaaggt
     3241 gcccgagacc gtcgacgtcc aaaagaccat ggagagcatg atcaatttgg acggcgccaa
     3301 gctacttttc ccgacctctt tcggctactt cgagcctcac atcctcaagc tcgccgagaa
     3361 gtatccccaa gtcacgttcc tgcactgcgg cgggctatat caggaaggca aacatccgaa
     3421 gaacgccgga agctacttcg gttacatcga tgaagccgag tacgtcgccg gtatcacggc
     3481 ggggctcacc accaagacga acaaactcgg attcgtcgcg gcgaagccaa ttccgcaggt
     3541 attgcgaaat atcaatgcct acaccctggg tgctcgttcc gtaaacccgc aggccgtgct
     3601 gaccgtggtg ttcacgggtg attggtcctt gcccgtgaaa gaggccgaag cggccaatag
     3661 cttgatcgat cagggcgcag acgtactgac cgcccacgtc gactccccga aagtagtgat
     3721 cgaaaccgca gaacgacgaa acgtctactc taccggatac cacgccagcc aagccgcatt
     3781 ggcacccaaa ggatacctta ccggtgcgga atggaactgg caggtcattt acaaaaagta
     3841 cgtggaggcg gttcagaagg gcgagactcc tgagcatctc gtgcgcggcg gattcaaaga
     3901 gggttacatc aaaatgtcgg cttacgggcc cgcggtcaac gacgctgccc gggccaaggc
     3961 ggatgaagcc aaagccaagc taaccgcagg ggatttcact atcttcaagg gaccactcaa
     4021 ggacaacagc gggaaggagc tgttggccgc ggggagcact caaatacaga ccgacataaa
     4081 cctggagaag atgaactact tggtggaagg cgtaaaaggc gcgttgaact gagcaggagc
     4141 attcatgtcg tctgcggcgt cgagcagctc gtccgaaacg ctgatcgcgg atcgcgagtc
     4201 atctccgcat gccctgcgtt ggctgctgat tcgcggtttg agctcgttgg aagagctgct
     4261 cgttccgttg ctcgggttca tggtctcgat gagcctgttc ggtgtgttca tcgcgctagt
     4321 gggcaagcct ccgctcgagg tgttcgcgca aatgtaccga ggtgcatttg gaaccacgtt
     4381 ctcgttccaa aacaccctaa tgcgcgcggc accgttgatg ctcacgggcc tttgcaccgc
     4441 tctgccggcg cgactcgggc taatcgtgat aggcggcgag ggggcgttgg tcgtcggcgg
     4501 ggtgacggcg gccgcggtcg ccccggccct gagcagcgcg agctcggtca ccatacaact
     4561 tgcgatggcc cttgccgcca cgatcatggg tgggctctgg atagcggccg ccggctggct
     4621 gaaggtctcc cgcggagtca acgagacgat tagcagcttg ctcctctctt acttggggat
     4681 cgcgttgatg aatcacctcg tcgaggggcc cctcaaggat ccggcgagtc tcaacaagcc
     4741 atctacgttt ccaattggcg aggccaacat gctacctaat ttgccgggca tggatctgca
     4801 ctacgggctg ctcctggggg tgctggcttg cttgctttgc tccctgttga tggaccaaag
     4861 ccgcttcggc ttcgcgagcc gcgtcgtggg tgggaacgtg cgagccgccc gtttggcggg
     4921 tctgccggta gcacgcctgg tagtgctggc ttgcttcttg ggcggagcgg cggccggact
     4981 agcgggaatg atcgaagtcg cagccgtcca cggcaaagcc aacggttcct tgatttgcgg
     5041 ctacggatac accggtattc tggtggcatt cttggctcga cacagcccgc tggcggtcat
     5101 gccggtggcg ctgctactcg gcggaatcag cgcgagcggc gggttgctgc agcggtcttt
     5161 cggtctaccg gacgccagcg tcaacgtgct ccaaggtgtc ttgttcgtgg tgctcctgac
     5221 gagcgagtcc tatcgaggga agctcaggcc ctttcggcac tggactcagc gagtttgagt
     5281 gagcgagttc caaggaccaa tgaacacact aaccaccatc gctgcaaacc cggaggcctc
     5341 tttagggcta ttgggggtgc cgctcgcggt gctcgccggt gcgctgcggg tcagtacgcc
     5401 gtttctgttc gtgagtttgg gagagtgcct gaccgaacgc tccggacggg tcaacttggg
     5461 tctcgagggc acactcgtga tgggagcgat ggtgggttat ggcggttcgt acctgagcgg
     5521 ctccccctgg ctcggggtgc tgggcgctgc cgcggtgggc gcgattctgg gggcgctgca
     5581 cgcctttttg tgcgcgcggc cccgggtgaa cgacatctcg gtcggcatcg cgttgatgtt
     5641 gctcggagtg gggttggcgt tctttctcgg taaaccgctc gtgcaaccga gcgccgcaag
     5701 actgccctcg atttccctcg gcttcttcag cgattcaccg caggttcgct ccgctctcca
     5761 ggtaaatgcg ttgttcttgc tgggattggt gctcgccccc actctacgct gggcgctcgc
     5821 gagcacacgt tggggcctcg tgttacgcac cgtcggggaa agcgcagagg cggcgcgcgc
     5881 gatgggctac gacgtcgccc gcgtccggct gctcgcaacg gcagccggcg gagcactcgc
     5941 cggcgtggga ggctcctttc tctctctatt ctatcccggc agctggaacg agggcctgtc
     6001 gagtggtcag gggttgatgg cagtggcgct agtcatcttc gcgcgctggg atcccgtccg
     6061 ttgcctttac gcctccttgt tgttcggtgg ggcgagtgcg cttgggccag cgctgcagtc
     6121 cgtgggcgta acccagggtt actatctgtt caacgcggcg ccgtatgcgc tgacactgct
     6181 catcatgatc ttgagtagct ccgccacaca cgcttcggcg gactcccctg ccgagctgac
     6241 gcgagttcgc tgagtagaaa catgaagcca gccgttcatt cgagccgagc cgttggcaac
     6301 gccaacacga gtgtcacctc gggccatgcc gctcccgacg tctcgtcgcg caccagctcg
     6361 ggaatccgcg tcaaggccgc accgcccgag ctcgaggtgc gcgccatgtg caagcgattc
     6421 gacagcttga ttgcgctcga cgacgtttcg ctgcgcctgc ggcccggctc atttcacgcg
     6481 ctgctcggag agaacggcgc ggggaaaagc acgctcgtca aatgcatcat gggcttctat
     6541 cgggcagatt ccgggagcgt cctggtcgac tcccagccca tcgaactgaa aagcccacgc
     6601 gaagcgcatc gccacgggat cggtatggtg taccaacact tcacgttggt agagaacatg
     6661 accgtcgcgg agaatctcgt gctctctcgc gccagagtgc ccgccgtcat cgactttgca
     6721 aaggaacggg ccgagctcga gagcttcatg gacaccatgc ctttccgagt tccctgcgag
     6781 aagcgcgtgc ggcagctcgc ggcaggcgag aagcaaaagc tcgagatcct gaagcagctc
     6841 tatctcggga gccgcatcgt cattttggac gagccgacga gtgttctgac gccgcaggaa
     6901 gctgacgagg tgctcggcct attgcgaaac atggcgcacg acggccgcat cagcgtcgcg
     6961 atgatcacgc acaagtttcg cgaagtcatg gccttcgccg acgaggtgac ggtcctacgc
     7021 cgaggccgcg tggtgggcgg tggcagcacg aacgagctca cccctgagac gatggccgag
     7081 ctcatggtgg gcagcaagcc cccggaaact cgcctggagc gagcaccgca ggccccggga
     7141 agaacgatct tggaactcga gggcatcacc gccggggacg acatcggcgt tcaggttctc
     7201 aacggagtaa gcctgcgcgt ccgtgcgggc gagattgtcg gcatcgcggg ggtgtccgga
     7261 aatgggcagg acgagctggt cgaggtgctc gccgggcaac gggcagcgag cgccggaagc
     7321 gtatgcgtcg acggcaagcc cttccgacca acacgcgccc agctgcgggc ccgcaacgtg
     7381 cgtctgttgc cggagtcacc gctgcgcaat gcatgcgtcg cgaacatgtc agtcgctgaa
     7441 aacatcgcgc tacgagtctt cgacacctct ccgttcacgc gattgagagc gttcgtgagc
     7501 cgcgctgcaa tgcgacgaaa cgcacgcgcg cgggtgaacg agtatcgcat caagaccccg
     7561 tcggtcgaca cgccgatcgg cgcgctctcg ggcggcaacg tgcaacgcgc cgtgctggcg
     7621 agggagctcg ccggcacggt agccctgctg atcgctgcaa atccgtgttt tgggctagac
     7681 ttcgcggcgg taagtgagat ccgcgagcga atcgtgaccg cacgcaactc aggggccgcg
     7741 gtactcttgg tgagcgctga tctcgaggag gtgttcgcgt tggccgatcg gatcttggtc
     7801 atgagcgcgg gtcgcatcgt attcgaaact gaagcgagcc tggccgatcc gaccgaaatt
     7861 ggtcgacata tggctggtca ttctggccct agtgaccggc ccgagtcact tccaggtacc
     7921 tagttgcaga tcagcaccac gccctcgtcg ggccccaatg ccacttccga tggcgtggag
     7981 cccagctcgc gctctgcacg ggtcgaaagc accagcttgg cgcgttcgac ctcagggttc
     8041 aataccggga gctgcggatg cgatgagaga ttgagcagga c
//
LOCUS       DASTKU010000790         7817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_824191, whole genome shotgun sequence.
ACCESSION   DASTKU010000790 DASTKU010000000
VERSION     DASTKU010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824191"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..793)
                     /locus_tag="VFQ61_27685"
     CDS             complement(116..793)
                     /locus_tag="VFQ61_27685"
                     /inference="COORDINATES: protein motif:HMM:NF016226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9958320.1"
                     /translation="MSDSFSSSSPGPDASARGYFQRIGASDAWNFILRAQEHSPTSIA
                     VFDVRSRKDFDARHVEGARHLTESTFPEVLREVAKSTAVVIYCYHGNASQSFAAMFAD
                     FGFPEVYSVDGGYEALLAEHDRRKNSLPEPEERYLVGDVVFAKQNIFNDGGVPDLPAE
                     ALLAEPGARGVVVRSGHVEADPQQVLYVVRFESADGRLGPPVGCLPDELTQVSPRQGA
                     DSPETGE"
     gene            1004..1522
                     /locus_tag="VFQ61_27690"
     CDS             1004..1522
                     /locus_tag="VFQ61_27690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958321.1"
                     /translation="MPPAASALESAWLSQRAYTDLPPEAERAIRRAGVCWHDEPAALR
                     HLEEARALAPDHLAVWIAQYRFHFYKHRYADAERCARVCLEQVALQLGVPPEITEVQP
                     THANFSADDALLRFWLFGVQAYGYVLIRLGQTARGYEVLRKLTQLDTSDCTKTRVLLD
                     VMQRPVEDEEGG"
     gene            1669..2283
                     /locus_tag="VFQ61_27695"
     CDS             1669..2283
                     /locus_tag="VFQ61_27695"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HET9958322.1"
                     /translation="MTADEKADAPGSHELPSRQTSDAEIREYTIGELQPWNDRIALRG
                     YDPEWPTAFEREAVRLRGILGHRIALLEHVGSTSVVGLPAKPIIDIVLALPDSSDEAS
                     YVPDLSASGYVLRIREPHWFEHRMFKGPDTDINLHVFSRGCPEVAQMLLFRDWLRSHA
                     QDRDLYARTKQELAERTWRFVQHYADAKTETIGAILARARAASP"
     gene            complement(2299..2685)
                     /locus_tag="VFQ61_27700"
     CDS             complement(2299..2685)
                     /locus_tag="VFQ61_27700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET9958323.1"
                     /translation="MTQTKALSSSETLSLTPAASKFLQRIVRFGGAGGDAGLRLEVSP
                     GGCSGMSSQFSVERNPFAGDEVVQFDGLKLFLPPPTVRLLAGVTIDFLETSTEAKLSF
                     VDPKAGACGCSSTAAPELPKGFIKLN"
     gene            2985..3512
                     /locus_tag="VFQ61_27705"
     CDS             2985..3512
                     /locus_tag="VFQ61_27705"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HET9958324.1"
                     /translation="MLQSIDSNAAPSHEAMAEAVGSAGSRACGVGLKLRLRIDVGGER
                     ILQFEAEAFGRAASAASSSQLRELFVNRTLRQALDLDHLEISRAIGGGSFCAVLVHEA
                     LLNAMAAYRGEIPRENGQIACRCYSVREGMIRRAVRRNQLTNIVQLAEFTRAGTACGD
                     CAGRLRHLLEQLTGT"
     gene            complement(3577..4518)
                     /gene="epsC"
                     /locus_tag="VFQ61_27710"
     CDS             complement(3577..4518)
                     /gene="epsC"
                     /locus_tag="VFQ61_27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HET9958325.1"
                     /translation="MTHAPVDDAAFESCGDMLSRVVGALCEANVSSLERPQTGRTLPS
                     REALSTVVNELRSALFPFHFGAPDVSRAGLSYFVGRTLDSALRSLERQVYYGLVFECG
                     HGGLPCSSCASRASEVLTAFAERLPQLRSLLGTDALAAYEGDPAAHSPDEAVFCYPGL
                     TAILHHRLAHELFSMGVPLIPRIISELAHASTGIDIHPGAKIGGRFFIDHGTGVVVGE
                     TCVIGNRVRLYQGVTLGAKSFPKDENGAYLKGAPRHPIVEDDVVIYAGATVLGRITLG
                     RGATIGGNVWLTESVEPGARITQAQSRRQWFDGGAGI"
     gene            complement(4515..5495)
                     /gene="cysK"
                     /locus_tag="VFQ61_27715"
     CDS             complement(4515..5495)
                     /gene="cysK"
                     /locus_tag="VFQ61_27715"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET9958326.1"
                     /translation="MANWFKDNAESIGRTPLVRLNRLVSADGARVLAKIEGRNPAYSV
                     KCRLGAAMVWDAEKRGLLGPGKEIVEPTSGNTGIALAFVAATRGYPLTLTMPETMSLE
                     RRKLLVAYGARLVLTEGSKGMTGAVAEAERIAASDPGRYVLLQQFKNPANPGIHECTT
                     GPEIWDDTGGEVDIFVSGVGTGGTITGVGRYLKHTRQKSVRVVAVEPAGSPVLSQRRK
                     GEPLKPGPHKIQGIGAGFIPDVLDLSLVDEVEQVTNEEAVEMARRLAREEGILSGISS
                     GAAVAAAVRLAKQPENAGKTIVVVLPDSGERYLSSILFEGVFDAQGLAAQ"
     gene            complement(5625..6737)
                     /locus_tag="VFQ61_27720"
     CDS             complement(5625..6737)
                     /locus_tag="VFQ61_27720"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9958327.1"
                     /translation="MSTDSPRHPSPHGVSDQSFRAHPLVELTKTRIREFLREKGLLFW
                     VFGFPILMAIGLGLAFRERPAELPRIAVVDDAQRPAARALLSSPELNAERMTHEQARR
                     AVSRAKVDVAVEWEGRAPSDASSTERPALHFDPLQDKSAYARAVVDSVLERHAGRKDL
                     VEEVLRPVTERGARYIDFLVPGLIALNIMGSSMWGVGFNLVLARKRKLLRRYAVTPMR
                     RSHFLLSYFFSRCVFLAMELTVLVAFGYFVFGSTVRGNLGVLALMAFSGAASFAAISL
                     MIGARVENTEAASGWMNFVQLPMWILSGTFFSYERFPHWLQVPIRFMPLTALNDALRA
                     VYSGEGNALGLLSHWLILMAWGAAGFLIAARTFRWQ"
     gene            complement(6734..7672)
                     /locus_tag="VFQ61_27725"
     CDS             complement(6734..7672)
                     /locus_tag="VFQ61_27725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9958328.1"
                     /translation="MSAPAIRCEALVKRYGEKVAVAGVDLEIPRGECFGLLGPNGAGK
                     TTTVELLEGLTPKDSGRIELFGIPWGTGRDRELRARFGVQLQHTELADKLTVEEVLTL
                     FRSFYPKGKRPEELIALLNLEEERRRQFCQLSGGQKQRVALGCALAGAPDLLFLDEPT
                     TGLDPRARKGLWGVVENFRNAGGTVLITTHYMEEAAALCDRIAIMDAGKIIALGTPRA
                     LVDALGLVQFLEFEVQSALDEAALRKLEVVQSLERRGERYRVRVARSLSALARVFDEL
                     ERQGVIPIGLSAHQATLDDVFLSLTGRGLENHTEAA"
BASE COUNT         1516 a         2543 c         2363 g         1395 t
ORIGIN      
        1 cgacggggtt ggcgttgaac tcgaccgagg tcagcgtgct cgcgggtaag ccaatgagat
       61 tcgtagagta ccagcggcgg ggatcgtctc gaacatcgac gacttccacg tgacctcact
      121 cgccggtttc gggggagtca gcaccttgac gtgggctgac ctgagtgagc tcatcgggca
      181 agcaaccaac gggcggcccc agcctgccat ccgcgctctc gaagcgtacg acgtagagca
      241 cctgctgggg atcggcctcg acatggcccg atcgcaccac cactccacgc gcgcccggct
      301 cggcgagcag ggcctctgcg ggcaggtcgg gcacgccccc atcgttgaag atgttctgct
      361 tcgcgaatac tacgtcaccc actagatatc gttcttccgg ctccggcagc gaatttttgc
      421 gccgatcgtg ctccgccagc aatgcctcgt agccgccatc cacgctgtac acctcgggaa
      481 atccgaagtc tgcaaacatg gccgcaaagg attggctggc gttgccgtga tagcagtaga
      541 tcacgaccgc ggtcgatttg gcgacttccc gcagcacctc ggggaacgtg ctctcggtca
      601 agtgccgagc tccctcgacg tgacgagcat cgaagtcttt gcgcgagcgt acgtcgaaga
      661 cggcaatact ggtcggggag tgttcctgtg cacgcaggat gaagttccaa gcatcgctcg
      721 ctccgatgcg ttgaaagtag ccccgcgcgg atgcatcagg gcctggggag gaagaggaga
      781 atgagtctga catgctgctc gggtcgttgg ttcgcgactc caaggcggtg tgcaggaccc
      841 gtgccgtgac ggctgcgcat tcgttactcc gacgctgaat gctcaggttg tcgggaacac
      901 gacaaatgcg acaggcttgc ggcctccgtt gtcatagagc cagcgagaag cgcaccacga
      961 gcgtcgccca tgttcggccc cgattttgcc tacgcgcgag cccatgccgc ctgctgcctc
     1021 cgcgctcgaa tcagcctggc tctcccagag ggcatacacg gacctgccac ccgaagcaga
     1081 gcgagcaata cgtcgcgcgg gcgtgtgttg gcacgacgaa cccgcggcct tgaggcatct
     1141 ggaagaagcg cgcgcgcttg cgcccgatca cctggcggtt tggatcgcgc agtatcggtt
     1201 tcacttctac aagcatcgct atgccgatgc ggagcgctgt gcacgagttt gcctcgagca
     1261 ggtcgccctg cagctcggtg tgccccccga aatcacggaa gtccagccga cgcacgccaa
     1321 cttcagcgcg gatgatgcgc tgcttcgttt ctggctgttc ggcgttcaag cctatggtta
     1381 cgtactgatc aggctggggc aaaccgctcg aggctacgag gtgctccgaa agctgactca
     1441 gctcgatact tcagattgca ccaagactcg agttcttctg gacgtgatgc aacgaccggt
     1501 ggaagacgag gaaggcggct agcacgacgc ccgtggggcc agggaagtgc cgaaatagct
     1561 gcgatccacg gggggctcgt gtccactctc tccctcggct ctgcacgtgt cttgtcagca
     1621 aagccgatgc atgctcagtg cagatctgat gcacctcggg attgagacat gaccgcggac
     1681 gagaaagccg acgcgcccgg ttcgcacgaa ctgccttcgc gccagacgag cgacgccgag
     1741 atccgtgagt acaccatcgg ggagctccaa ccctggaacg accgcatcgc cctgcgcggg
     1801 tatgatccgg agtggcccac cgcattcgaa cgcgaagccg tgaggcttcg tggcattctc
     1861 gggcatcgca tagctttgct ggagcatgtg ggctccacct cggtggtggg cttgcccgca
     1921 aagccgatta tcgacatcgt gctcgcgctg ccggattcgt cagatgaagc gagctacgtc
     1981 cccgacctgt ccgcctccgg atacgtgctc cgaatccgtg agccccactg gttcgagcat
     2041 cgcatgttca aggggcccga tactgacatc aacttgcatg tcttcagccg cggctgcccc
     2101 gaagtcgcgc aaatgctgct gtttagggac tggctcaggt cccacgctca ggaccgtgac
     2161 ttatacgccc gaaccaaaca ggagctcgca gaacgaacct ggaggttcgt tcaacactac
     2221 gcggacgcca agacagagac gatcggagct atcctcgctc gcgcccgcgc agcatcaccc
     2281 tgagcgcctc ggcagctgtc aattcagctt gatgaagccc ttgggcagtt cgggcgcggc
     2341 ggtcgaactg cacccgcagg cacccgcctt gggatcgacg aacgagagct tggcttccgt
     2401 cgacgtctcg aggaagtcga tcgtcacgcc cgccagcaag cgcacggtcg gtggaggcaa
     2461 gaacagcttc aatccatcga actgaacaac ctcgtccccc gcgaatggat tgcgctcgac
     2521 actgaactgt gaggacatcc cggagcagcc tccgggactc acttcgagtc gcaggccggc
     2581 gtcgccaccc gcgcctccga agcgcacgat gcgttgtaga aacttcgatg cggcaggggt
     2641 caacgacagc gtttcggagc ttgagagtgc cttggtctga gtcatacgat gagtcctgag
     2701 aacatcctga aaactggacc ggcgcagtgc acgacacgtg ccagtgccga ctggcgattc
     2761 tcccgcgctc ggcgagcccg cagccttcga aagaagacgg ttccagcgcc gatcggcgct
     2821 cgttccgagg ggcaacgctg gcgtcgcaac tgaaggaaac cctacaaagc agacagcagc
     2881 gcgtacgcca gttctgggcc gagactcttg cacagttgcc agttatccgt agttgcccat
     2941 caatgttgct ggcacgtgcg ttgcctcgct tcgaatgaag aacgatgctc caatcgatcg
     3001 attcgaatgc cgcgccctcg cacgaggcca tggcggaggc cgttggttcg gcaggctctc
     3061 gcgcctgcgg cgtgggcctc aagctgcgct tgcgcatcga cgtcgggggt gagcgcattc
     3121 tccagttcga ggcggaggca tttggtcgcg ccgcctccgc ggcgtcttcg tctcaactgc
     3181 gcgagctttt cgtaaaccgc acgctgaggc aagcgctgga cctggatcac ctggaaatct
     3241 cgcgcgcaat tggtggcgga agcttttgcg cggtgctggt gcatgaagct ctgctcaatg
     3301 ccatggcggc gtatcgcggt gagatcccgc gcgagaacgg gcaaatcgcc tgccgctgct
     3361 acagcgtgcg agaaggcatg attcgccgtg cggtgcgcag gaaccaactg accaacatcg
     3421 ttcagctcgc cgagttcact cgggcgggta cggcgtgcgg agattgcgcg ggccggctcc
     3481 gccacttatt ggagcaactg accggcacgt gaagccaaga gctgcccggt tcgaaacctt
     3541 cgatttcgaa gccgagcagc tcgagtctta gccaggctaa atcccggcgc cgccgtcgaa
     3601 ccactgacgc cgagactgcg cctgcgtgat tcgggcccca ggttccacgc tctcggtgag
     3661 ccacacgttt cccccgatcg tggccccgcg gccaagcgtg atccgcccga gtacggtcgc
     3721 ccctgcatag atgaccacgt catcctccac gattggatga cgtggtgctc ccttcaggta
     3781 agctccgttt tcgtctttgg ggaagctctt cgcgcccagg gttacgcctt gatagagccg
     3841 cacgcggtta ccgatgacgc atgtctcgcc caccaccact cccgtgccat gatcgatgaa
     3901 gaaacggccc ccgatcttgg cccctgggtg aatatcgatc cccgtggaag cgtgcgccag
     3961 ctccgaaatg attcgtggga tcagggggac acccatcgag aacagttcgt gcgccaagcg
     4021 gtggtgcaga atggcggtta gcccaggata acaaaacacg gcctcgtccg ggctatgggc
     4081 ggcgggatct ccctcgtaag cggcgagcgc gtcggtcccc aataggcttc gaagctgcgg
     4141 caaacgttcc gcgaacgcgg tcaacacttc gctcgcgcgc gacgcacacg agctgcaggg
     4201 cagcccgccg tgcccgcact cgaagaccag accgtagtaa acttgccgct cgagactacg
     4261 cagcgccgag tcgagagtcc gaccgacgaa gtacgagagc cccgctcgag agacgtccgg
     4321 cgccccgaag tggaaaggaa acagagccga acgcagctca ttgacgaccg tggacaaagc
     4381 ctctcgggaa gggagggtcc gcccggtttg aggccgctcc aacgagctga cgttcgcctc
     4441 gcacaacgcg ccgacgacac ggctcaacat atccccgcac gactcgaacg cggcgtcgtc
     4501 cacgggagcg tgagtcattg ggccgccaag ccctgggcat cgaacacgcc ctcgaacagg
     4561 atgctactca agtagcgctc tcccgagtca ggcagtacca cgacgatggt tttcccggcg
     4621 ttctccggtt gcttcgcaag ccgcacagcc gcggccacgg ccgcgccgct agaaattccg
     4681 gagaggatgc cctcctcccg cgccagccgt cgggccatct cgacggcttc ctcgttcgtc
     4741 acctgctcga cctcgtccac gagcgacaag tcgagcacat cggggatgaa gccggccccg
     4801 atgccttgga tcttgtgcgg gcccggcttg agcggctcac ctttcctacg ttgcgacagt
     4861 accgggctgc ccgccggctc caccgcaacg actcgaaccg acttctgacg ggtgtgcttc
     4921 aagtaacggc ccacgcccgt gatggtgccg ccggtcccga ctcccgatac gaagatgtcg
     4981 acctcaccac ccgtgtcgtc ccagatttcg gggcccgtgg tgcactcgtg aatgcctgga
     5041 ttggcagggt tcttgaattg ttgaaggagc acatagcggc caggatcaga agccgcaatt
     5101 cgttcggctt ccgcaaccgc gcccgtcatg cctttcgagc cctcggtcaa caccaggcgc
     5161 gctccgtagg caaccaggag cttgcgccgc tcgaggctca tcgtctccgg catggtgagc
     5221 gtgagcgggt aaccgcgggt ggccgctacg aaagcgagtg cgatccctgt atttccgctg
     5281 gtcggttcga ctatttcctt gcccggaccg agtagcccgc gcttctccgc gtcccacacc
     5341 atcgccgcac ccaaccggca cttcaccgag taggctggat tgcggccctc gatcttcgcg
     5401 agcacacgag cgccatccgc agataccaac cgattgagcc gcacgagcgg agttcgccca
     5461 atcgactccg cgttgtcttt gaaccaattc gccatgtaac gagccttcct actcgcgcag
     5521 ggtaatgcaa gtggagtgcc tgcgccgcca cttcagaccg agagtcttcc aggcggctgc
     5581 cggaacgcag ccagcaccaa acggacgggc agagatggac cgaactactg ccagcggaag
     5641 gtgcgcgcgg cgatgaggaa acccgcggcc ccccatgcca tcaggatgag ccaatgtgag
     5701 agcaacccga gggcgttgcc ttcgccggag tacactgcac gcagcgcgtc gttgagcgcc
     5761 gtcaacggca tgaaccggat tgggacttgc agccaatgcg ggaagcgctc gtaagaaaaa
     5821 aacgttccgg agaggatcca catcggtagt tgaacgaagt tcatccagcc gctggcggcc
     5881 tcggtatttt cgactcgggc cccgatcatc agactgatcg ccgcgaacga tgcggcgccg
     5941 gagaatgcca tcagcgcgag cactcccagg tttccgcgca ccgtgctgcc aaacacgaag
     6001 taaccgaacg ccaccagcac cgtgagctcc atggcaagaa acacgcagcg ggaaaagaag
     6061 taggacagca agaagtggct tcgccgcatc ggagttactg cgtaccgacg caggagcttg
     6121 cgcttgcgcg cgagaactag gttgaaccca accccccaca tgctgctgcc catgatgttc
     6181 agggcgatca ggccgggaac caggaagtcg atgtagcgcg cgccgcgctc cgtaacgggg
     6241 cgcaggacct cctcgacgag atctttcctg cccgcgtggc gctcgagcac tgaatccacc
     6301 accgcgcggg cgtacgctga tttgtcctga agtggatcga agtggagtgc ggggcgctcg
     6361 gtgctcgagg cgtcagacgg tgcccggcct tcccactcga cggccacgtc caccttggcc
     6421 cgtgagacgg cgcgccgagc ttgctcgtgg gtcatgcgct cggcgtttag ctctggacta
     6481 ctcagcagcg cccgcgccgc cgggcgctgc gcgtcgtcca ccaccgcgat ccgcggcaac
     6541 tctgccggcc tctcccggaa cgccaagcca aggccaatcg ccatcaagat cggaaagccg
     6601 aacacccaaa acagcagccc tttctcgcgc agaaactccc gaattcgcgt cttggtgagc
     6661 tccaccaatg ggtgcgcgcg gaaagactga tccgaaacgc cgtgcggcga agggtgacgt
     6721 ggagaatccg tgctcacgcg gcctccgtgt ggttttcgag cccacggccc gtgagagata
     6781 gaaacacatc gtccaaagtg gcctgatgcg cgctgagccc gatcgggatc acgccctgtc
     6841 tttcgagctc gtcgaacacg cgcgccagcg cagataggct gcgcgccact cgtacgcgat
     6901 agcgctcgcc tcggcgctcg agcgactgca ccacctcgag cttgcgcaaa gccgcttcgt
     6961 ccagcgcgga ctgcacctcg aattccaaga attgaaccaa ccccaacgca tccactagcg
     7021 cgcgcggagt tccgagcgcg atgatctttc ctgcgtccat gatcgcgatg cggtcacaca
     7081 gcgcagcggc ttcttccatg tagtgcgtgg tgatcaagac cgtgccaccg gcgttgcgaa
     7141 agttctccac gactccccac agcccttttc gagcgcgcgg atcgaggccg gtggtgggtt
     7201 cgtccaaaaa caggagatcg ggcgccccgg cgagtgcgca acccaaagcc acgcgctgtt
     7261 tttgcccacc ggatagctgg cagaactggc gtcgtcgctc ttcttctaga ttcaagagcg
     7321 cgatcaactc ttcggggcgc ttgccctttg gatagaagga acggaagagc gtgaggactt
     7381 cctccaccgt gagcttgtct gcaagctcgg tatgctgcag ttgcacaccg aagcgtgccc
     7441 tcagttctcg atcccgtccc gttccccacg ggatgccgaa gagctcgatt cgaccagaat
     7501 ctttgggcgt caagccttcc aaaagctcca cggtcgtggt cttgcccgca ccgttcgggc
     7561 ccagcaggcc gaaacactcg ccgcgcggga tctcgagatc tactcccgcc acagccacct
     7621 tttcaccgta acgtttgacg agggcttcgc aacgaatggc cggcgcgctc atgttgcggc
     7681 gcagtatacc tacatccgct cattctccga gactctcacg tgccaagagc ccctcgaacg
     7741 acccttcgtg ggtgccggga tcgccatcgc gcaacctggt ttgcgcgagg aagcgcagtc
     7801 tttcccgagc caccggg
//
LOCUS       DASTKU010000791         3162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1496331, whole genome shotgun sequence.
ACCESSION   DASTKU010000791 DASTKU010000000
VERSION     DASTKU010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496331"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VFQ61_27730"
     CDS             <1..1195
                     /locus_tag="VFQ61_27730"
                     /inference="COORDINATES: protein motif:HMM:NF015617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-reducing end alpha-L-arabinofuranosidase
                     family hydrolase"
                     /protein_id="HET9958329.1"
                     /translation="ASGGATQSGGAIGTGGSASGGRVNGNGGAGNSAGGAAGSSNGGA
                     PASGGGPASGGKTAGGAGGQGAGGQASGGASGGGGTASGGTSASGGAGAGGGGMACPL
                     PTSFKWKDNGGPLATPANGAVSMKDFTSTVSNGKHVVYMTTYSTNWGAAMMSFADWSG
                     AATATQSKLSFTAVAPTLFYFTPKQTWVLAYQWGAHGFDYRTSSDPTNPSGWSGEKSL
                     YSSKDVPSGSSSTGVIDQQVICDTAKCYLFFAGDNGYIYRSSMAIGSFPGEFPPATTV
                     MQDTSAKLFEAVQVYSIKGANKYLMLVEAPSGHPRYFRAFTATDLGGSWTLLTDTFAS
                     QSNTTFSQKWTSDISHGDLVRQDPSELFTVDPCNLQLLYQGTSTTSGDYNTLPYRPGL
                     LTLEK"
     gene            1412..2503
                     /locus_tag="VFQ61_27735"
     CDS             1412..2503
                     /locus_tag="VFQ61_27735"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9958330.1"
                     /translation="MTAWRRCITTFVFSTSVCLHACGKASHDKLLGSDGGEAGSFSAA
                     GGNDNLGGADLGGVSQTGGADAEGCRSNANGSDLCQSDLVPGGAMERTEERLDQDGNP
                     TSAQLSVSDFYLDRFEVTVARFRKFVAAYPGSRPAAGAGAHPKLPNSGWSAHWDGANA
                     LPETKAALLSRLHCPQNSWLVVDSGWDPVGPSCDDRNATWSDTPGSAEDAPIYPVSYY
                     IAFAFCAWDGGRLPTEAEWEYAAAGGSERRAFPWGDGAPSANYANYSEGAASVFVAVG
                     NTPLGDARWGQRDLAGSVDEWVLDAYGTLPKGCDDCANVNPFALVHTRGGNYVSDAEG
                     IRVAARPNNQWRATEFRAGFRCARASARH"
BASE COUNT          578 a          981 c         1032 g          571 t
ORIGIN      
        1 cgccagtggt ggagcgactc aatccggtgg cgcgatcggg acgggcggaa gcgcctctgg
       61 gggtcgggtc aatggcaacg gcggcgccgg caacagcgcc ggtggagccg ctggttcttc
      121 gaacggcggc gcgcccgcca gcggtggcgg ccccgcaagc ggaggcaaaa cggccggagg
      181 cgcgggcggt caaggcgcgg gcggtcaagc ctcgggcggt gcgagtgggg gcggcggcac
      241 ggcgagcggt gggacgagcg cctctggcgg cgccggtgcg ggcggtgggg gcatggcgtg
      301 cccattgccc acctcgttca agtggaaaga caacgggggg ccactcgcca cgccagcgaa
      361 cggcgcggtc tcgatgaagg acttcacgag taccgtctcg aacggcaagc acgtcgttta
      421 catgaccact tactccacca actggggcgc ggcgatgatg agcttcgccg actggtccgg
      481 agccgcaacc gctacgcaaa gcaagttgtc attcacggcc gtcgcgccga cgctgttcta
      541 cttcactccc aagcagactt gggttctcgc ctaccagtgg ggcgcccacg gtttcgatta
      601 ccgcacgtcg tccgatccga cgaacccgag cggttggtcc ggcgagaagt cactgtactc
      661 gagtaaggac gtgccgtctg gctcgagctc gaccggcgta atcgatcagc aggtcatctg
      721 cgacacggcg aagtgttacc tcttcttcgc cggtgacaac ggttacatct acaggtcgtc
      781 gatggcgatc gggagctttc cgggcgagtt cccccccgcc accacggtca tgcaagacac
      841 gtcggcaaag ctcttcgagg ctgttcaagt ttactcgatc aagggcgcta acaagtacct
      901 catgctcgtc gaagccccgt cgggtcaccc gcgttacttc cgcgcgttta cggcgacgga
      961 cctcgggggc tcgtggacgt tgctgaccga cacctttgca agccagagca acacgacgtt
     1021 cagccagaaa tggacctccg acatcagtca cggagacctc gttcggcagg atccgtctga
     1081 actcttcact gttgatccct gcaatttgca gctcttgtac caaggcacgt cgacgacgag
     1141 cggagactac aacacgcttc cctaccgacc tggtttgttg accctggaga agtagccgcc
     1201 cgagcacaga acgcgagtcg aggcacgcgc caaccccggc gcgtgccttc cgcaccgtca
     1261 ttgggtcgcc tcaaccgcgc aacgcgctgc cactgtccgg tctttctagt ccactggtga
     1321 gggaggcccg gggagagagc gaggcctggt ccgcttgcgc aaatgaagat gcgcttttcg
     1381 ttctgcggcg agctgttaag gtcatcgaca catgactgcg tggaggcggt gtatcacgac
     1441 cttcgtcttc tccacgagcg tgtgccttca cgcctgtggc aaggcgagcc acgataagtt
     1501 gcttggctcc gacggcggag aagcagggtc tttcagcgcc gcaggaggca acgacaacct
     1561 cggcggtgct gatcttggtg gcgtaagtca aaccggcggt gccgatgccg aagggtgtcg
     1621 gtccaacgct aacggctcag acctgtgcca aagcgactta gttccaggcg gcgcgatgga
     1681 acgcaccgaa gaacgcctgg accaggatgg caatccgacg agcgcgcaac tcagtgtctc
     1741 ggatttctac ctcgataggt tcgaggtgac cgttgcgagg tttcgcaagt tcgtggctgc
     1801 ttatcctggc tcacgacccg ctgcgggggc gggcgctcac cccaagctcc cgaacagcgg
     1861 ttggtcggct cattgggacg gagcgaatgc gttgcccgag accaaggctg cccttctctc
     1921 acgccttcat tgcccacaga acagctggct agtcgtcgat tccggctggg atcccgttgg
     1981 cccgagctgc gacgatcgca acgcgacttg gtccgatact ccggggagtg cggaggatgc
     2041 gcccatctat cccgttagtt actacatcgc gtttgcgttt tgcgcatggg acggcgggcg
     2101 cctaccgacg gaagcggagt gggagtacgc cgcggctggc ggcagcgagc ggcgcgcatt
     2161 cccttggggt gatggagcgc cgtctgccaa ctacgccaac tacagcgaag gcgcggcctc
     2221 cgtcttcgtc gccgtcggca acacaccgct tggagacgcg cgctgggggc agagggacct
     2281 agcgggtagc gtcgacgagt gggtgctcga cgcatacggc acgctgccga aggggtgcga
     2341 tgactgcgcg aacgtgaacc catttgctct cgttcacacg cgcggtggca attatgtgag
     2401 cgatgccgaa ggaattcgcg tcgcggccag gccgaacaac caatggcgag cgaccgagtt
     2461 ccgcgccggc ttccgctgtg cgcgggcgag cgcccggcac tagaacctca gcgtcgtcag
     2521 cgtcgtcagc gtcgtcaaag cgacgtgtag ctcgtgtggc ggctcagagg tgcaccaagg
     2581 gtgacaaggg agcaatcggg tgaggcagtt ccgcaagcgc atgcgccacc cttggcgctt
     2641 gtgcctcccc ccgatctcac tcagagcacg accgcacgct ccaccccgcg tggttacatc
     2701 gctggttctt cggctcatca aagcgtccgc ggtgccatcg ctctgtgcgc cgccgggctt
     2761 cagtaccgca ggccgcgcgg cttgccgtag caatcgacgt cacgagctgc gtgataaatc
     2821 gcgcccgagg ttacggttcc ctttaccgaa acgtgtcggt ctcgagtttt acgctaggca
     2881 tggcttgtac ggtgagaacg ctcgcaagaa aaacccgagt ggcatccgcg acgtcgagta
     2941 tcgagctcgt catcaggttg tcatcgcagt gggttcgccg tcaggtatcg accatcgccg
     3001 aacaaggtag atcatgaaaa accgctggat cctgggcctg cttcttccac ttactgcttg
     3061 cgggaacaaa ggtggctcga cgaacggagc gtccggcggc tcgggccaaa tcagcacggg
     3121 cgggagcact aacgcttcgt ccggcggtgc ggccagctcg tc
//
LOCUS       DASTKU010000792         4370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1496651, whole genome shotgun sequence.
ACCESSION   DASTKU010000792 DASTKU010000000
VERSION     DASTKU010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496651"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFQ61_27740"
     CDS             complement(<1..198)
                     /locus_tag="VFQ61_27740"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9958331.1"
                     /translation="MAIYKHLLVPTDFEVCSRHALERAMAMATAFSSKLTLLHVWEPP
                     VYPYVVPAAPDYVTAIEEAVKH"
     gene            718..1680
                     /locus_tag="VFQ61_27745"
     CDS             718..1680
                     /locus_tag="VFQ61_27745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958332.1"
                     /translation="MTVSTVVPVAHAETSEADRATARALAEEGHKALAAKDYALAEDR
                     FRRADALIRAPSLMVDHARALVGLGRLAEAYEDFDAARKEKLAPGAPPAFKRAVAEAE
                     RAIKEIAPKIAWVTLRVNGPSDPLIKIGEREIPASALGAPLPVDVGAQTIQVSAEGYV
                     PKDAPLSLAAGQKTELELTLEPVPVAPPAPPPAPVIKHVVRRQPPPPVESKPSALTYI
                     AFGVGGAGLAVGAVTGILCLSERSKLSSECPDGKCAPGTHHDDIARYHLYSYTSAIGL
                     LVGVAGAGTGVALLLTKPKHEEPHAKARVTPYVGLGSVGVEGKF"
     gene            1680..>4370
                     /locus_tag="VFQ61_27750"
     CDS             1680..>4370
                     /locus_tag="VFQ61_27750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958333.1"
                     /translation="MHSRPTQTRSWLVVLSTTLLLLFGCHVVLGDFEESPAPPPVSGL
                     CTKGAFRCKDELLLTCNADQSAWMLKDTCASKEQCSSDRGACLVCPQADALRCNGASL
                     EICNSKRVSWDFKEMCPTAELCSPGGCGPCAVPGALQCAVDPSTMMPVIRECDPVNKI
                     WKAVVSCNNADLCDATVTLAQTASATWDRRCDFVCQPGTFRCDGANLQRCPQNGVNWM
                     AASSCASPALCQLTLDKISKDPNVAASTDMCEPGCGTPGQLRCVNGTSLQRCSEDSTA
                     WQPIVDCPAGKQCSTEGQGSCIVCTPGHYQCNGSFLERCDDQNTWKQVQDCKTSALCQ
                     FQNDTADPSKSTGRCDAPACPRAGDNVCGTDTDPKASGATLWECKPDLTGYVKGLTCE
                     TAQLCHATDKACALPLCKSGARRCNPKNLLEVQECNGGETDWNSVTTCKSGEICDASD
                     KQQPCKHDCPAPLLCNQRVLQTCSAAGGVQNKAQCDTNELCACAIASPPCATKTDGCG
                     SPVCTAGTYRCTGSVLEQCNPGRDGWSRVADCGASNLCYPGPSPAFTNGYCAVCPPGG
                     EVQCMGSATQTCSADRKSWINLTSCSFGCVNSSTVDYCGQCFPNEKRCSGGQVLQCSG
                     DQKVLTPIANCQYGCVDSGNNDYCAACVANERRCTGTAAGSRVQLCSADQTSLADTGS
                     PCTIGCVDSGTNDYCAECVNGQTECVGTSVRTCTNGKWSAASACPNGACVDSGTSDYC
                     PGCSKGELRCSGDALLACSADQKTMSPVAESPCANGCVDANLADYCAACRSSELRCMG
                     STLQQCSTDRKSLALVKDCGASGCVDNGTTDYCAVCKKGETRCMGSMLQACAADQKSL
                     MDVMACTMGPCVDAGDADYCGECKPGDTKCTTDGYVTCGAD"
BASE COUNT          793 a         1355 c         1467 g          755 t
ORIGIN      
        1 gtgcttcact gcctcctcaa tcgccgtaac gtagtcgggt gcggctggta ctacgtaggg
       61 gtagacgggg ggctcccaaa cgtggagcag cgtgagcttg gaagagaaag cggtcgccat
      121 cgccatcgca cgctcgaggg cgtggcgaga gcaaacctcg aagtcggtcg ggacgaggag
      181 gtgcttgtag atggccatgg gaacaccttt cggggtggga gtcgcgccgg ggcgctccgc
      241 tgctagctta cgctcattca cgcgaaactg acgcgtgcgc gctgcgcacg gtgagttcga
      301 ccgacctcct tcggcgagga ggcttcgcga agcttggtgc gtcggctcat ctgggttggt
      361 gcgtcggcgg tgccactcga gcaggttcgc cacagcggcg cctgctcgcg aaacttcgag
      421 tgaacgtgcg aatcgaggcg aaatggccga gcgcccatcc actcgaaagc gcgagtgttt
      481 tcggacgcga aagggccgac cgtcggcggg gcaatccttg cgaaagtcaa ggaaatcgtg
      541 gtaagacagg ctacgctacc ctcttacact cacttacgaa cttggtcggg gcagcggtca
      601 tcgactgcgg gacacggaac ggcgggcaat cgcatgtccg actttcaaac gacagcttgc
      661 tctcgctcgc gcgggagtat tttcaaactt tgttcagcgg tggggttgtt gctcgccgtg
      721 acggtgtcga ctgttgtgcc ggtcgctcac gcggagacca gcgaggccga tcgcgcgacg
      781 gcgcgcgcct tggcagagga agggcacaag gcgctcgccg ccaaagatta cgccctcgcc
      841 gaggatcgct ttcgccgcgc cgacgctttg attcgggcgc cgtcgttgat ggtcgatcac
      901 gcgcgtgcgc tggtcgggct gggccggctc gccgaggcct acgaggactt cgatgcggcc
      961 cgcaaagaga agctcgctcc cggagctccg cccgctttca agcgagccgt ggccgaggcg
     1021 gagcgtgcga tcaaggagat cgcgccgaag attgcgtggg taacgctgcg cgtgaatggg
     1081 ccgagcgacc ctctgatcaa gatcggcgag cgcgaaattc ccgcgagcgc tctcggtgcg
     1141 ccgctgccgg tcgacgtcgg ggcacaaacg attcaggtct cggcggaagg ctacgtcccg
     1201 aaggacgcgc cactttcact cgccgcgggc cagaagaccg agctcgagct gacgctggaa
     1261 ccggtgccgg tcgccccgcc tgcgcctcca cctgctcccg tgatcaagca cgtggtgcga
     1321 aggcaacctc cgccgccggt agaaagcaaa ccgagcgcgc tgacgtacat cgcattcggc
     1381 gtgggcggcg caggtctcgc ggtaggagcc gtcaccggca tcttgtgcct cagtgagcgc
     1441 tcgaagctgt cctcggagtg cccggacgga aagtgcgcac ccgggactca tcacgacgac
     1501 atcgctcgtt accacctgta ctcgtatacg tcagcgatag gtctcctcgt cggcgttgct
     1561 ggggcaggta cgggggtcgc gctcctcctg acgaaaccca agcacgaaga gccgcacgcc
     1621 aaggcgcgtg tgacgccgta cgttgggctc ggctccgtgg gcgtggaggg gaaattctga
     1681 tgcacagccg tccaacccaa acgcgctcgt ggctcgtggt cctgtcgacc acgctgctct
     1741 tgctcttcgg ctgtcacgtc gtcctcggcg acttcgagga atcgccggcg cccccaccgg
     1801 ttagcgggtt gtgcaccaaa ggcgccttcc gctgcaaaga cgagctccta ctgacgtgca
     1861 acgccgatca gtcggcgtgg atgctgaagg acacctgcgc ttccaaggag cagtgcagct
     1921 cggaccgcgg cgcgtgcctc gtgtgtcccc aagcggacgc gctgcgctgc aacggagcat
     1981 cgctcgagat ttgcaattcg aagcgcgtca gctgggactt caaggagatg tgcccgaccg
     2041 ccgagctttg cagcccgggc gggtgcgggc cttgcgcggt gccgggcgcg ctccagtgcg
     2101 cggtcgatcc ttccaccatg atgccggtga ttcgcgaatg cgatccggtg aacaagatct
     2161 ggaaggccgt cgtcagctgc aacaacgcgg acctgtgtga cgcgaccgtc acgctcgcac
     2221 agacggcttc agctacctgg gatcgtcgct gcgatttcgt ctgtcagccc ggcacgtttc
     2281 gctgcgacgg ggccaacttg cagcgttgcc cgcaaaatgg cgtcaactgg atggccgcga
     2341 gcagctgtgc gagccccgcg ctctgtcagc tcacgctcga caagatttcg aaggatccca
     2401 acgtcgctgc ctcgaccgac atgtgcgagc cgggctgcgg cactcccggg cagcttcgtt
     2461 gcgtcaacgg gacgtcgctg caacgctgca gcgaggattc gacggcgtgg cagcccatcg
     2521 tggactgtcc ggcaggcaag caatgctcga ccgaggggca aggctcctgc atcgtctgca
     2581 ctccagggca ctaccagtgc aacggttcct tcctagagcg gtgtgacgac cagaacactt
     2641 ggaagcaagt ccaggactgc aagacttcgg cgttgtgtca gttccaaaac gacaccgccg
     2701 atccgagtaa gtcgacgggg cgctgcgacg ccccggcgtg tccgcgcgca ggcgacaacg
     2761 tctgcggcac cgatacggat ccgaaagctt ctggcgcgac gttgtgggag tgcaagcctg
     2821 acctcaccgg gtacgtaaag ggtctgacgt gtgagacggc gcagctctgc cacgcgaccg
     2881 acaaggcttg tgcgctgccg ctctgcaagt cgggcgcgcg ccgctgcaat ccgaagaacc
     2941 tcctggaggt tcaggagtgc aacgggggtg agaccgattg gaacagcgtc accacgtgca
     3001 agtcgggtga gatttgcgac gccagcgaca agcagcagcc ctgcaagcac gactgtccgg
     3061 ctccgctgct ctgtaaccaa cgcgtattgc agacctgttc ggccgcgggc ggcgtgcaga
     3121 acaaggctca gtgcgatacg aacgagctct gcgcatgcgc gatcgcgagt ccgccttgcg
     3181 cgaccaagac ggacggttgc ggttctccgg tgtgcacggc gggaacctac cgctgcaccg
     3241 gttcggtgct cgagcaatgc aatcctgggc gcgacggctg gagccgtgta gcggactgtg
     3301 gtgcctcgaa cctctgttac ccagggccca gtcccgcgtt caccaatggc tactgtgcgg
     3361 tgtgccctcc cggcggggag gttcagtgca tggggtcggc cacgcaaacc tgctccgcgg
     3421 acagaaagag ctggatcaac ctgacgagct gttcgttcgg ttgcgtgaac agcagcaccg
     3481 tcgactactg cgggcaatgt ttcccgaacg agaagcgttg ttcgggaggg caggtgctcc
     3541 agtgctccgg cgaccagaaa gtgctgaccc cgatcgcgaa ttgccagtac ggctgcgtgg
     3601 actccgggaa caacgactac tgcgctgcct gcgtcgcgaa cgagcggcgt tgtaccggaa
     3661 ctgccgctgg ctcccgagta cagctctgct cggcggatca aacgtcgctc gcggacacgg
     3721 gatcgccctg cacgattgga tgcgtcgaca gcggcacgaa cgattattgc gccgaatgtg
     3781 tgaacgggca gaccgagtgc gtcgggacca gcgtgcgcac gtgcaccaac ggtaagtgga
     3841 gcgctgccag cgcttgtccg aacggtgctt gcgtcgatag cggcacgagc gactactgcc
     3901 ccggatgctc caaaggcgag ctccgatgct ccggcgacgc gttgttggcg tgcagcgccg
     3961 atcagaagac gatgagcccc gtggcggagt cgccgtgcgc gaacggctgc gtcgacgcga
     4021 acctcgccga ttactgcgcc gcgtgtcgca gtagcgagct tcgctgtatg ggctccactc
     4081 tccagcagtg cagcacggat cgcaagtcgc tcgcgctcgt aaaggactgc ggcgcgtcag
     4141 gctgcgtcga caacgggacg accgactact gcgctgtctg caaaaagggc gagacgcgct
     4201 gcatgggctc gatgctgcaa gcttgcgcgg cagatcaaaa gagcctgatg gacgtcatgg
     4261 cttgcacgat gggcccctgc gtggacgctg gcgacgcgga ctattgtggc gaatgcaagc
     4321 ctggggacac caagtgcacg accgatggtt acgtcacctg cggcgccgac
//
LOCUS       DASTKU010000793         3728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1718656, whole genome shotgun sequence.
ACCESSION   DASTKU010000793 DASTKU010000000
VERSION     DASTKU010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718656"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFQ61_27755"
     CDS             <1..367
                     /locus_tag="VFQ61_27755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958334.1"
                     /translation="GTPPLHRTLITRAQAAACVQRRAFELFAARGVHRTVGRTGLLVV
                     ISELERHVVILGDSKIQELIGHEGFQEHVQKLIQRLKSGEGARGILELIEDFRPLLAQ
                     HAPRKIDDVNELPDAVIRA"
     gene            454..1407
                     /locus_tag="VFQ61_27760"
     CDS             454..1407
                     /locus_tag="VFQ61_27760"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HET9958335.1"
                     /translation="MMLPAARTQPLDTTLGWVLIRELLSAARDCAFPPRKIGVSLDHS
                     GRLEYREPEASPVWVEFASEPGFGYSPELSRDVQQMLELYLPLCVGRSAHELVLAHMG
                     QSLDGQIATATGDSRYITGRENLEHVHRLRALCDAVMVGADTADLDDPKLTTRLVPGQ
                     SPVRVVIDPELRVSRQRALFTDGAPTFVVCRAGAVRDALGGPGVEFVEVEPGSQRMSA
                     HAIVGALRARGLRRLFIEGGGVTVSRFLHEGALDRLHLTVSPVFVGQGRPGVALPPLA
                     ALSDALRPSTRHYPLGPDVLFDCALKRRPWLPSERRTVSTS"
     gene            1421..2431
                     /locus_tag="VFQ61_27765"
     CDS             1421..2431
                     /locus_tag="VFQ61_27765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9958336.1"
                     /translation="MRQAFVVPDLDGPATGGTLYNARLIRALTSLGCDVMVSAGATPL
                     LDALGEVDCVWVDSLYLNQVPILRRRVPDAAALGLLVHYLPTLLEQRAPLSPADLSEA
                     ERQALACAGRLLTPSPFMAELLAGLVPNHVPIDVVEPGCEVQLAPWPPRASGRVSVLM
                     VCNLLPNKRVAEFLEALSRSVVPANLALCIAGSADLDAAYAARCLKLVHDHPALRAQV
                     MFAGPVDPAQLQTWYANQDLLLSASLFESYGMALAEARAAGLPILACTGGNVANLVDE
                     NAGGELSASPEQLACSLLALCASPEELARRRHLARIRAPVPRSWQLAATEFAEALGCA
                     RR"
     gene            complement(2472..3251)
                     /locus_tag="VFQ61_27770"
     CDS             complement(2472..3251)
                     /locus_tag="VFQ61_27770"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9958337.1"
                     /translation="MPVLLQEGQAPAPAPAREEAVRHSPARPTHAFLLAWSARHAVLV
                     ASSTVATQVLLATQRLEAQRGLLMLVWAVPIALSFAKLWFRAPAETASGARFAAPNWI
                     TAARLVSAVGLCATGASDRALSLGVFLVWAMDGLDGYLARRLERTSELGAWFDMESDA
                     MLVLVASLGYVRGGAPGYIVATAGMLRYWYLLITARFPPRRQVPRSRIARLAFGAHVL
                     LLCAGLAFHDPVARWVLGFLGSAVVLASFGRSFVYAYRNEP"
     gene            3250..>3728
                     /locus_tag="VFQ61_27775"
     CDS             3250..>3728
                     /locus_tag="VFQ61_27775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958338.1"
                     /translation="MLRALSDSAYKSSTARAFWIERPGTGELREERLAALQPGELCVQ
                     TLFSAVSRGTESLVFQGQVPRSEWQRMRCPYQVGEFPGPLKYGYINVGRVVAGAPAWL
                     GRPVFCLYPHQTQYVVTEAAVTPIPDHVPLERAVLAANLETALNGVWDGLPRLGDRV"
BASE COUNT          638 a         1172 c         1230 g          688 t
ORIGIN      
        1 gggcactccg ccgttgcatc gcaccctcat cacccgagcc caggcggctg cctgcgtgca
       61 gcggcgcgcg ttcgagctgt ttgccgctcg aggggttcac cgaacggttg gacgcactgg
      121 gctactggtc gtcatttccg aactggaacg gcacgtggtc atcctgggtg atagtaaaat
      181 tcaagaactg ataggccacg aagggtttca ggaacacgtc caaaagctga tccagcgcct
      241 caaatccggg gagggcgcgc gcgggatctt ggaattaatc gaggatttta ggcctcttct
      301 cgcccaacac gcgccgcgta aaattgacga cgtgaacgag ttaccggatg cagtaatccg
      361 tgcctaatcg aaagtgttcc aggccgtcct gcgcggcgcg ctgttttttt tccgggaacc
      421 gcgctcgacc atgagacact gtagtcgact agcatgatgc tacctgccgc gcgcacgcag
      481 ccgctcgaca cgacgctggg ttgggttttg attcgggaac tcctgagcgc ggcgcgcgac
      541 tgcgcttttc ccccgcgtaa gatcggcgtg agcctcgatc attcgggaag gctcgagtat
      601 cgagagccag aagcgagccc ggtttgggtg gagttcgcga gcgagcccgg gttcggatat
      661 tcgccggagc tttcccgtga cgttcagcag atgctcgagt tgtacttgcc gttgtgcgtg
      721 ggccgctccg cacacgagct ggttttggcc cacatgggtc aaagtttgga cgggcaaatc
      781 gcgaccgcga ccggggactc acgctacatt accggacggg aaaacttgga gcacgtgcac
      841 cgcctgcgtg ctctgtgcga cgcggtgatg gtcggcgcag acaccgctga cctggatgat
      901 ccgaagctca ccacgcgctt ggttcccggg cagagcccgg tgcgcgtggt gatcgatcca
      961 gaactacgag tatctcgtca acgcgcgcta ttcacggatg gggctccaac cttcgtcgtc
     1021 tgccgcgcgg gcgcggttcg cgacgcactc ggcggcccgg gggtggagtt cgtagaagtg
     1081 gagcccggct cgcaacggat gtcagcgcac gcaatcgtgg gcgccttgcg cgcgcgcgga
     1141 ttgcgccgcc tattcatcga aggtggcggc gttacggtgt cgcgcttttt gcacgaaggc
     1201 gcgctggatc gactgcattt gacggtatca ccggtcttcg tcggtcaggg ccggccaggg
     1261 gtcgcgcttc caccactcgc ggccttgagt gatgctttgc gcccctcgac tcggcattac
     1321 ccgctaggtc cggacgtgct gttcgactgc gctctcaagc ggcggccctg gcttccgagc
     1381 gagcgaagaa cggtctccac ttcgtgaagt gggagccacc gtgaggcaag ccttcgtcgt
     1441 tcccgatctg gacgggcccg ccaccggcgg caccctctac aacgcgcggt tgatccgagc
     1501 cctgacatca ctggggtgcg acgtcatggt gtcggctggc gccacgccgt tgctcgacgc
     1561 cctaggcgag gtggattgcg tctgggtgga tagcctgtac ttgaatcagg ttcctatcct
     1621 gcgtcgtcgt gttccggacg cggcagcctt gggtctgttg gttcactact tgccaaccct
     1681 gctcgagcaa cgcgcaccgt tgtcgcccgc ggacttatcg gaagctgagc ggcaggcttt
     1741 ggcgtgcgca ggacgcctgc tcacgccgag cccgttcatg gccgagttat tggcaggcct
     1801 ggtcccgaac cacgtcccga tcgacgtggt cgagccgggt tgcgaggtgc agctggcgcc
     1861 gtggccaccc cgtgcatccg gccgggtgtc ggtattgatg gtgtgcaacc tgctgccgaa
     1921 caagcgggtg gccgagttct tggaggcctt gagtcgcagc gtggtgcccg ccaacttggc
     1981 cttgtgcatc gcggggagcg ccgacttgga cgcggcgtat gccgcgcgtt gcttgaaact
     2041 cgtgcacgac catcctgcgc ttcgagctca ggtgatgttc gcaggccccg tggacccggc
     2101 gcagctccaa acttggtacg cgaatcagga cctcttgctc tccgcttcac tcttcgagtc
     2161 gtatgggatg gcgctcgcag aagccagggc cgccgggttg ccgatcttgg cgtgtacggg
     2221 cgggaacgta gccaacctag tcgacgagaa tgcaggaggg gagctgagtg catctccgga
     2281 gcagctcgca tgttcccttc tggccttgtg tgcgtccccg gaagagttgg cccgccgtcg
     2341 gcatctcgcg cgcatacggg ctcccgttcc gcgctcttgg cagctcgccg ccacagaatt
     2401 cgcagaagca ctgggctgcg ctcgtcgata gggtggagga tgacggttgg gccacgactc
     2461 tcgagtcagc gttacggctc gttccggtac gcatacacga aagagcgtcc gaacgaggca
     2521 agcaccaccg ctgaacccaa gaagcccaac acccagcgcg cgacgggatc gtggaacgcg
     2581 agccccgcac acaggagcaa aacgtgtgct ccgaacgcga gcctcgcaat gcgtgagcgc
     2641 ggcacttgcc ggcgaggagg gaagcgcgcc gtgattagca agtaccaata acgcagcatc
     2701 ccggctgtcg cgacgatgta gcccggcgcc cccccgcgca cgtagccgag gctggccact
     2761 agcaccagca tggcatccga ttccatatcg aaccatgccc cgagctccga agttcgctcc
     2821 aaccgccgcg ccagatagcc gtccaagcca tccatggccc agaccaggaa aactccgagt
     2881 gagagggcgc ggtcactcgc gccggtggcg cacaagccca cggccgagac gagccgcgcg
     2941 gccgtgatcc agttgggagc ggcaaagcgg gccccactgg cggtctcggc cggagctcgg
     3001 aaccacagtt tggcgaagct gagcgcaatg ggcaccgccc aaacaagcat cagcagcccg
     3061 cgttgcgctt cgagccgctg cgtcgcgagc agtacctgcg tagctaccgt gctcgatgcc
     3121 actagcaccg cgtggcgtgc gctccacgcg agcagaaacg catgcgtagg gcgagcgggc
     3181 gagtggcgca cagcttcttc tcgcgccgga gccggcgccg gggcttgacc ttcctgaagc
     3241 aacaccggca tgttacgcgc gttgagcgat tcggcataca aatcgagcac cgcgcgcgcc
     3301 ttctggatcg agaggccggg caccggcgag ctgcgcgaag agcggctcgc ggcgttgcag
     3361 cccggtgagt tgtgcgtcca gaccctgttc agcgccgtaa gtcggggcac cgagagcttg
     3421 gtgtttcaag gtcaggtgcc gcgctccgag tggcagcgga tgcgatgccc ttaccaggtt
     3481 ggcgaatttc cgggaccgct caagtacggg tacatcaacg tcggtcgcgt ggtcgcgggc
     3541 gccccggctt ggcttggtcg cccggtattt tgcctctacc cgcaccagac ccaatatgtc
     3601 gtcacggaag cggcggtgac tccgattccg gatcacgtgc cgttggagcg tgccgtgctg
     3661 gccgccaatc tcgaaacggc gctcaatgga gtttgggacg ggctgccgcg cctcggtgac
     3721 cgagtcag
//
LOCUS       DASTKU010000794         6268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_378128, whole genome shotgun sequence.
ACCESSION   DASTKU010000794 DASTKU010000000
VERSION     DASTKU010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378128"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2562
                     /locus_tag="VFQ61_27780"
     CDS             <1..2562
                     /locus_tag="VFQ61_27780"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HET9958339.1"
                     /translation="VALDTRDPSAITLSGKFEIPGNISDSRIVGDILYVAAYENGACW
                     GCATVPRTNVMSLNVKNPASVSKVDQLSFEERTDSYSWKRSLTVTDRRMFVAGPRYGS
                     EGPLGSVIQVIDISDPSGDMKEGANVEVFGQIDSRWQMDEYEGVLRVISQPLQWRSSD
                     APQVQTFTVESSQAIAPLASMELKLPRPEALQSVRFDGTRAYAITFERKDPLFTIDLA
                     DAAHPVQKGELEMPGFLYYMEPRGDRLIGLGYDQGNKDGALTVSIFDVADLAKPSMLS
                     RVNFGGNWAWLAEDQDRIHKAFNVLDDAGLILVPFSGSYLEDSVDACSYRYQSGVQLI
                     DWQSDSLALRGVAPAVGQARRGLIHDGRLLAMSDERVEMFDITNRDEPVTTATLRLAQ
                     NVQRSVPAGDNVVRIAQNWWTQNTELDVVPLTQASFPSDVPSLEIPELGGGKNSCYSS
                     SWLGSANSSGKDVYLAYQTYEYDPAANQSYSRMNVVTVDASKPQSPKVSGRATLAEQF
                     GYGWSSYGMVDTGAALVNIGSTLAFVDRQVRYDTGNTPFLERSNLVLADMTDPNQPAT
                     QSVAMPTGNGATGLIASGNVVATSHYEGVEGGARFYLDRVELTANGAKALPKINIPGS
                     LVAYDAKTERAITVDYAQQVVPTITLKDCYERFAFPSFDIGQGTYTEDALGTCTAIIQ
                     SVNLIEIRDGKAHVLGRIVLKESERVGAVAQGDDRVFMTLGGAGYYYRLGVPSIGIAD
                     GASCFGCGTSFESGTLPLLTVSGLTSGQFVTGRIELESGDYYSGASIAAAGQRAVLAT
                     GFRGKLSVISAVDSAHPVLVREAEVPGYVQDLRVISNIAVASLGTDGVQTVDLAD"
     gene            2850..3641
                     /locus_tag="VFQ61_27785"
     CDS             2850..3641
                     /locus_tag="VFQ61_27785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958340.1"
                     /translation="MFATLSFIAMQRMDRPVWSEISDSTDSGGARSESGFSRSLASFA
                     LGQTQRTEVQAANATLDAADLELIRQQPFCVASGVWSFGKISEVVRFVGRRDGDQLRV
                     GYFVHWSAERPWGNNMLTYTLLPALTLDLVYSHFLFVLPGLREAAYGASDVEGVSVTY
                     AAQGDGLRVLHGDADDGVHTPIQLDAEELTTPDQRVVVMSEVWSHQLGARRASQRLDS
                     AASLTCFSGRRLQPLTRELARGFRLGSVEHPRRARPAWRALTLPE"
     gene            complement(3710..4234)
                     /locus_tag="VFQ61_27790"
     CDS             complement(3710..4234)
                     /locus_tag="VFQ61_27790"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET9958341.1"
                     /translation="MKTSVNSALDTIVQSATVELFHSQGIALGPIPRTQSTAMKLRQA
                     GLIGLINFTSPSMSGSLGLALSADVLSSAPDGPKQEHAVRDWLRESTNQLMGRIKNRC
                     LQFQVTLQAGLPSIVNGEALLRQSPRNETELLYMFRALRGEVSVSLRGRLDTKSLVYS
                     GSVSVASEGELILF"
     gene            complement(4415..5989)
                     /locus_tag="VFQ61_27795"
     CDS             complement(4415..5989)
                     /locus_tag="VFQ61_27795"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9958342.1"
                     /translation="MRSVRGAAFSAYSPLLFASLSLVACSGSETGVGLGSAGPLGGAN
                     TSAGAASFPGNGSTAGINGISGGFTSTGTGSSSLGSGGQTTPQGGASTNKGGASNGGA
                     GSGGASGGSAGSSTPPPPPPPPPALCELKLKCPQDIGNDTKIACTLEVEDGVGKLVYQ
                     GSAGVERRGRSSQKFPKKNYSVELRNASGTDLGIDLLGMGKDSDWVLDGSWADRSFMR
                     NALVFDSYAALGKPHYAAESRFCHLQLNDKSQGVYRLSERIKRDASRVSIAADDGTGQ
                     SFIIKQDSEGELRFPLGLEDSWELVYPKQKSATAAQKQAVQAWLDGFASALRGGNPSD
                     ATKGVFSYLDLNSTVDYVLLQEFAKNIDAFTLSSHLVRDAGGKASFIPWDLDLSFGQP
                     TVGPSSEDPQGADNDTPTGFIVHRPDLIQILAKVPEFKSKLGPRYRELRRSVLSESML
                     RQRIDAYLVSLEASALVENFKLFPIEEVDYTQLYEPYSFYRVSSHAEEITHLWTFIQQ
                     RLTWLDAHIDAYPNAN"
BASE COUNT         1135 a         1920 c         1982 g         1231 t
ORIGIN      
        1 gttgcgctcg atacgcggga cccgagcgcg atcacgctct cgggtaagtt cgaaatcccc
       61 ggcaacatct cggactcccg catcgtcggc gacattctgt acgtggccgc gtacgagaac
      121 ggcgcgtgct ggggctgcgc caccgtgccg cgcacgaacg tgatgtccct gaacgtcaag
      181 aacccggcca gcgtgagcaa ggtggaccaa ctgagcttcg aggagcgaac cgacagctac
      241 tcctggaagc gcagcctcac cgtcacggat cgcaggatgt tcgtagccgg gccacgctac
      301 ggctccgagg gcccgctcgg ctcggtgatt caagtcatcg atatttccga tcccagcggt
      361 gacatgaagg agggggccaa cgtcgaggtg ttcgggcaaa tcgacagccg ctggcaaatg
      421 gacgagtacg agggcgtgct gcgggtcatc agtcagcccc tgcagtggcg ttcgtcggat
      481 gctccgcagg tgcagacgtt cacggtcgag tcctcgcaag ccatcgcgcc gctcgcgtcc
      541 atggagctca agctgccccg ccccgaggcg ctgcagagcg tccgcttcga cggcacgcgt
      601 gcgtatgcca tcacgttcga acgcaaggac ccgctcttca ccatagattt ggccgatgca
      661 gctcatcccg tacaaaaggg tgagctcgag atgccgggct ttctctatta catggagccg
      721 cgcggcgatc gcttgattgg tctcggctac gatcagggca acaaggacgg cgcgctcacg
      781 gtctcgatct tcgacgtcgc ggatttggcg aaaccgagca tgctcagccg cgtcaacttc
      841 ggcggaaatt gggcgtggct ggccgaggat caggaccgta tccacaaggc cttcaacgtg
      901 ctcgatgacg cgggcctgat cttggtgccg ttctccggct cctatctaga agactcggtg
      961 gatgcttgca gctaccgcta tcagtctggc gttcagctga tcgactggca gtcggattcg
     1021 ctcgccttac gcggggtagc cccggcggtg ggtcaggcgc gccgcggcct gattcacgat
     1081 ggtcgactgc tcgcgatgag tgacgagcgc gtcgagatgt tcgacatcac caaccgcgac
     1141 gaacccgtga cgaccgcgac gttgcggctc gcgcaaaacg tgcagcgcag cgtgcctgcc
     1201 ggcgacaacg tcgttcgcat tgcccagaat tggtggacgc agaataccga gctggacgtg
     1261 gtgccgctca ctcaggcttc cttcccgtcg gacgttccga gcttggagat tccggagctg
     1321 ggcggcggca agaactcctg ttactcctcg agctggctgg gcagcgcgaa ctcttcgggt
     1381 aaagacgtct acctcgcgta tcaaacctac gaatacgatc cggcggccaa tcagtcctac
     1441 tcgcgcatga acgtggtcac cgtcgatgcg tcgaagccgc aatctccgaa ggtgagcgga
     1501 cgcgcgacgt tagcggagca gtttggctac ggctggagct cctacggcat ggtggatacc
     1561 ggggcggcgc tcgtgaacat cggatcgacg ctggccttcg tcgaccgtca ggtgcgctac
     1621 gataccggca acacgccctt cctcgagcgc tccaacttgg tgctcgcgga catgaccgac
     1681 ccgaaccagc ccgctacgca gagtgtggcg atgccgacgg gtaacggcgc gaccggtttg
     1741 atcgcgagcg gcaacgtggt ggcaacgagt cactatgaag gcgtggaggg cggagcacgc
     1801 ttctacctag accgcgtgga gctcaccgcg aatggcgcga aggcgctgcc caagatcaac
     1861 atccccggtt cactcgtggc ctacgatgcc aagaccgaac gcgcgatcac cgtagactac
     1921 gcgcagcagg tcgtcccgac catcacgttg aaagactgtt acgagcggtt cgcgttcccc
     1981 tccttcgata tcgggcaggg cacctacacg gaggacgcgc tggggacctg cacggccatc
     2041 atccagagcg taaacctgat cgagatccga gacggcaaag ctcatgtcct cgggagaatc
     2101 gtgctcaaag agtccgagcg agtcggagcc gtcgcccaag gtgatgaccg cgtcttcatg
     2161 accctcgggg gcgcaggtta ctactatcgt ctgggtgtgc cctcgattgg catcgcggat
     2221 ggcgcgagct gtttcgggtg tggcaccagc ttcgagagcg gcacgctccc gttgctcacg
     2281 gtatctggtc tgacctctgg gcagttcgtt accggtcgca tcgagctcga gagcggggac
     2341 tactacagcg gagcatcgat tgcggccgct ggtcagcgcg cggtcctcgc caccgggttc
     2401 cgcggcaaac tcagcgtgat ttccgcggta gattcagcac atccggtgct cgtgcgagag
     2461 gcggaggtgc ccggctacgt gcaagacttg cgggtgatct cgaacattgc cgtggcctcg
     2521 ctcgggacgg acggcgttca gaccgtcgat ttggccgatt gaagtaattg gcagtaaggg
     2581 tgccgagggc gctcggcgcg gggcgtcaca caagagcttc cgccgagcgc tttcgtgttc
     2641 gtgagttggg tactgtgcgg acattccgac ttcacctttg cgggtgatct gccgtacctt
     2701 gggtgggcgt tcgtcgaagt gtcgcgcgag ccacttagga gtggctgcga gtcgccgtct
     2761 atctccccac cctttacatg catcgtgtca gatacgcggc ctgtgcgaga tctcggcgtg
     2821 tttcgaagac ttgccggatc tgggctcgag tgttcgcgac gctttcgttc atcgccatgc
     2881 agcgcatgga tcggccggtg tggtcggaaa tcagcgactc gaccgacagc gggggagctc
     2941 gatccgaaag cggattttct cgcagtctcg caagtttcgc gcttgggcag actcaacgca
     3001 ccgaagtgca agcggcgaac gccacgctgg atgctgcgga tctcgagctg attcgacaac
     3061 agcccttctg cgttgcctcc ggcgtgtggt cgttcggtaa aatctcggag gttgtccgct
     3121 tcgtgggccg tcgtgatggg gaccaactgc gcgtcggcta cttcgtgcat tggagcgcgg
     3181 agcggccgtg gggcaacaat atgctcacct acacgttgct cccggcgctc acgctcgacc
     3241 tcgtctacag tcatttcctt ttcgtgctcc cgggtttgcg agaagccgct tacggcgcct
     3301 cggacgtgga gggagtgtcg gtgacgtacg ctgcgcaggg ggatggcttg cgtgtgttac
     3361 atggtgatgc cgatgacggc gtgcatacac cgattcagct cgacgcggag gagctcacca
     3421 cccccgacca gcgcgtcgtc gtcatgagcg aagtgtggag tcaccagctc ggggcccgtc
     3481 gtgcgagcca gcgcctcgac agcgcggcga gcctgacttg cttcagcggg agacgcttgc
     3541 agccgctgac tcgtgaactg gcccgcggct tccggctcgg ttccgtggag catccgcgcc
     3601 gcgctcgacc ggcgtggcgc gcgctcacgc ttcccgagta ggagttgcgc acggggcgcg
     3661 cgcgctcggc ttggtccgag ttcccgtcgc gccggcggga agtccgggct tagaacagga
     3721 tcagttctcc ttcgctcgcg acgctcaccg agcccgagta aacgaggctc ttggtgtcca
     3781 aacgcccacg caggctgacc gagacttctc cgcgcagggc gcggaacatg tagagcagct
     3841 ccgtctcgtt gcgcggtgat tgtcgaagta gcgcttcccc gttcacgatc gagggcagcc
     3901 ccgcctgcaa cgtgacttga aattgtaggc agcggttttt gatgcgcccc atcaattgat
     3961 tcgtcgactc gcgcagccaa tcgcgcacgg cgtgctcttg cttgggaccg tccggggccg
     4021 agctcagcac atctgccgat aaggccagcc ccaggctgcc tgacatgctg gggctcgtga
     4081 agttgattag accgatcaac cccgcttgtc gcaacttcat tgcggtagac tgggttcgcg
     4141 gaatcggacc gagcgcgatg ccttgcgaat ggaagagctc gacagtcgca ctttgtacga
     4201 tggtatcgag cgccgaattg acactcgtct tcaacgcagt cctctagttt tcacgcggcg
     4261 gaggtatgtt cgatggactc aaacgtgcct tcaggcgaca ggctctgcgt cgtccgatgt
     4321 ctggcgttgg cgctccgaga aacggtcgcc gtgcgagacg ccataagtac cgatcgggta
     4381 cgtagcggga ggtgctatat ccgcgcgcgg ctagtcagtt tgcgttcgga taagcgtcga
     4441 tgtgcgcgtc gagccacgtc agccgttgct gaatgaaggt ccacaggtgc gtgatctctt
     4501 cggcgtggga gctcactcgg tagaacgagt atggctcgta cagctgggtg tagtcgacct
     4561 cttcaatcgg gaagagtttg aaattctcga ctagcgcgct ggcttccaga gacaccaagt
     4621 aagcatcgat gcgctgacgc agcatgctct ccgagagcac gctacgtctg agctctcgat
     4681 agcggggccc gagcttgctc ttgaactctg gcactttggc gaggatctgg atcaaatccg
     4741 gacgatgtac gatgaagccc gtcggcgtgt cgttgtcggc gccttgggga tcctcagaag
     4801 atggccccac cgtgggttgt ccgaaggaca aatccagatc ccaggggatg aagctggctt
     4861 tgccgcccgc atctcgcacc aggtgagagc tcaaagtgaa ggcgtcgatg ttcttcgcga
     4921 actcttggag cagcacgtag tcgacggtgc tgttcaggtc gagataactg aagacgccct
     4981 tggttgcatc gctgggattc cctccgcgga gtgcgctcgc gaagccgtcg agccaggcct
     5041 gaaccgcctg tttttgagcc gcggtggcgc tcttttgctt cggataaacc aattcccaac
     5101 tatcctcgag gccgagcgga aagcgtaact cgccttcact atcctgcttg atgatgaagc
     5161 tctggcccgt tccgtcgtcg gccgcgatcg agacgcggct ggcgtcgcgc ttgatgcgct
     5221 cgctgagccg atacacgcct tgggacttgt cgttcaactg caagtggcag aagcggctct
     5281 ccgcggcgta atgcggctta ccgagtgcgg cataggagtc gaataccagc gcgttgcgca
     5341 tgaacgagcg atccgcccaa gagccgtcca aaacccaatc cgagtccttc cccatgccga
     5401 gtaggtcgat gcccagatcc gtcccgctcg cgttgcgtaa ctcgacggag taattcttct
     5461 tggggaattt ttgggaagag cggcctcttc gctcgactcc agcgctaccc tggtacacga
     5521 gcttccccac gccgtcttcc acctccagcg tgcacgcaat cttggtgtcg ttcccaatgt
     5581 cctgaggaca cttcagcttg agctcgcaca atgcgggcgg cggtggtggg ggtggcggcg
     5641 gggtggagct tccggcgcta ccgcccgagg ctccaccgct acccgcgcca ccattcgacg
     5701 cgccgccttt gttggtgcta gctccaccct gaggggtagt ctgcccgccg ctacccaagc
     5761 tggagctacc cgtcccggtg ctggtgaatc cgccagaaat cccgttgatg ccggcggtcg
     5821 agccgttgcc tgggaacgag gccgcacctg cgctcgtatt cgcgccaccc aacggcccgg
     5881 cgcttcccaa gccgacgcca gtttcggaac cgctgcaagc gacgagggat agagacgcga
     5941 agagtagcgg agagtaagcc gagaaggcag ctccacgaac ggaacgcacg aagggagacg
     6001 cacccacgcg tcggagaacc gagttcaccc tgcgcagttt atcccaaact tccgactgca
     6061 cacgtgatct atgaaggttc cacgacgctg ctcatccctc acttcagcgt caccaagact
     6121 tcaaaaactc cctctgggcg tagttcgctt cccgaaacgc tcgcgttgcg cccttggagc
     6181 gcgctcgaag cttgcgtccc agcgggcgtc ggaccacggt gctcgcgcta ggagctggcc
     6241 atgacttcgc ccacgaatcc gcaagaaa
//
LOCUS       DASTKU010000795         6369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1048402, whole genome shotgun sequence.
ACCESSION   DASTKU010000795 DASTKU010000000
VERSION     DASTKU010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048402"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="VFQ61_27800"
     CDS             <1..1342
                     /locus_tag="VFQ61_27800"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9958343.1"
                     /translation="PSLALVAALGIYACHNVTERAHDRYRSPEGPSNAGGTAGETSNF
                     AGAGASSGSTTSPGSSFGAEGDGTVSSGGSPDDPANGPAPVDGVHTGGSGGKEPLTNS
                     TSAGEGGTPPLPSRCVPGAACQRIEDCWLGAVRCEGDRAICAPARPAPEHTRCIYGEC
                     TRDGSCIKAMPSCRNLDTPGCGALTVEGGTFRMGYSAGDPATPAGEPVTVDTFALDIH
                     EATVARFRQFWATQSALVRDVTFPNGVHRKVETSPHEPEPNSAHYSYNWTPEPWERED
                     HPINRLTWATAFLFCAWDGGRLPTEAEWEYAATGREIDGLKRGRDYPWGNQLPNCTLA
                     NYLSCGSGTTVSVNSFEPWGGFYQMAGNVTEWTADSFTYVPGACWDGSPRENPVCVEE
                     GSPFFVAKGGSYAGGDGQMRGVWRLKSQNGVGPRGVRCARDLLGNRPTVSSAAQ"
     gene            complement(1449..2114)
                     /locus_tag="VFQ61_27805"
     CDS             complement(1449..2114)
                     /locus_tag="VFQ61_27805"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9958344.1"
                     /translation="MNEDVDATQSEIRNGSPLDPAARPYENLMVYVSGCGGLLLGNSS
                     GTASYVLTARHCTDDGLPAIRRPDGSIPGGGAKRVIQHPLALAGGDDTVDAALVELKR
                     PEPTTSRVTFSNLTPQELAGQQARCYGAGNIENYGINTGVFTLAIDHRAPNSSIYYDV
                     VLPNELGQYVSKGDSGGPCVRVTGTQDTTVFGINKAGDKYTFARQTAAVAFSEWVRTY
                     VPR"
     gene            complement(2660..3289)
                     /locus_tag="VFQ61_27810"
     CDS             complement(2660..3289)
                     /locus_tag="VFQ61_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6151 family protein"
                     /protein_id="HET9958345.1"
                     /translation="MDGDVELRCRCGKVHGWLKGTAPSTVNRMVCYCSDCQAFLHHLG
                     RADLMDAHGGTEIVQVPPNSISYDRGTELIAGVRLAPGGMHRWYASCCKTPLGNTRTP
                     ALPFVGLPHEVLFGAPSPERRTEAFGPNRGAVALERAIGGIPEGVVKPKASSLLRVVW
                     LLLGWKLSGKAWPHPFFERNAPGPKFPLTELSPAEREGLRSRCGPNPSV"
     gene            3549..4493
                     /locus_tag="VFQ61_27815"
     CDS             3549..4493
                     /locus_tag="VFQ61_27815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958346.1"
                     /translation="MILIALGIELRSRHRLAHFADLTARERRQLEAAKPRSVYAEPLP
                     GDARRELDVILGAARVPSSVKLQPQDLARPEGLSSAQQAALDGLSPVMPQVSRALHMN
                     HRSGLREAFSYKTAFAIHAALARAGERRLPTEPAAGTRQILDALVFGAELGHNGSVFP
                     ALVGASLLEASCAQIERLWNQLDPELRHAALAQTSLPPPQAWLMGEKLLHFSTLLELS
                     QANFGFEDGPTLLAPPSRRLEITKALFAAETFFEQANRALSLQPPSSERCEQAYLSLQ
                     REVQLGSLTPTNVCSQVQKFTRAQQALERLRTRAGSIR"
     gene            4909..5538
                     /locus_tag="VFQ61_27820"
     CDS             4909..5538
                     /locus_tag="VFQ61_27820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958347.1"
                     /translation="MSIKQCILGICVGWSGLALAGCSSGGALEGASADGGDGDTAATE
                     EALGSVSCSTATADKTLDGSADPTHVSPSTYNTCYRGYVVDINNLSVSYTGVKSASSG
                     YDAHISVDWKGPVPTTKSACESAWGSAIFYKKVGGSWVDQSGVLQAYGEWQDRGDLVS
                     FCSPPSITTLDVLTLEAGASYRVAATMRTTYGGSTLRALGVTTRKKVSF"
     gene            complement(6082..>6369)
                     /locus_tag="VFQ61_27825"
     CDS             complement(6082..>6369)
                     /locus_tag="VFQ61_27825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958348.1"
                     /translation="LLAIATPFITLAGLSAHLFDSQLGRALDRWAISIGLACLAFPLG
                     TELAVTYSRHTLDDTIVRLGAPVILFTAIPGALISLLALAVSSHRARRLST"
BASE COUNT         1194 a         2083 c         1972 g         1120 t
ORIGIN      
        1 acctagtttg gctctcgtcg cggctctcgg catatatgct tgtcacaacg ttacagagcg
       61 agcccacgac aggtatcgga gccctgaagg gcccagcaac gctggcggaa ctgccggcga
      121 aaccagcaat ttcgcgggag cgggagcatc ttccgggagc accacctccc cgggcagctc
      181 gttcggcgct gagggggacg ggaccgtctc ttccggcggg tctccagatg acccggcgaa
      241 cggccccgct cccgttgacg gtgttcacac cggcgggtcc ggcggcaagg agcccctgac
      301 gaactccacg agcgccggcg aaggaggcac cccaccgtta ccttctcgct gcgtgcccgg
      361 tgcagcgtgc caacgcatcg aggattgttg gctcggtgca gtgcgctgcg aaggagaccg
      421 cgccatctgc gctcctgcgc gacccgcacc ggagcacacc cgctgcattt acggcgagtg
      481 cacgcgtgat ggcagctgca tcaaagccat gccgagctgc agaaacctcg acactccagg
      541 ttgcggagcc cttaccgtcg aaggcggcac gtttcgcatg gggtatagcg ccggtgatcc
      601 ggcgacccct gcgggcgagc cggtcacggt agacaccttc gcgctcgaca ttcatgaggc
      661 aaccgtcgcc aggtttcggc agttttgggc aacacagtcc gccttggtgc gggacgtcac
      721 ttttccgaat ggggttcacc ggaaggtaga gacatcgccg cacgagccgg agcccaacag
      781 cgcacattac tcctacaatt ggacgcctga gccctgggag cgagaagacc atcccatcaa
      841 tcgcctcacg tgggccaccg cctttctatt ttgcgcgtgg gatggtggtc gactgcctac
      901 cgaagcagag tgggagtacg cggccacggg ccgagagatc gatggactca agcgggggcg
      961 cgactatccg tggggcaatc agctgcccaa ttgcacgctg gccaactacc tgtcttgcgg
     1021 cagcggcacg accgtgtccg taaatagctt cgagccctgg ggcgggttct accaaatggc
     1081 tggcaacgtg acggagtgga ccgcggattc tttcacctac gtgcccggcg cttgctggga
     1141 cggtagcccg cgcgagaatc cggtctgcgt cgaagagggc agccctttct tcgtggctaa
     1201 ggggggcagc tatgcgggcg gcgacggtca gatgcgcgga gtgtggcgcc tcaaatcgca
     1261 aaacggcgta gggccgcgtg gggtccgttg tgcacgtgat ctactcggca atcgtccgac
     1321 ggtgagctca gctgcgcaat gacgcgatga cgcacggctt tgcgtgctcg ttggcggtcg
     1381 gcggatgggc gagcaggccg ggcgacatgg cgccgacctg ctctcgtcac gttccttagt
     1441 ccagcgcttc agcgcggcac ataagttctc acccactcag agaacgcgac ggctgccgtc
     1501 tgccgcgcaa acgtgtactt gtcgccggct ttgttaatcc caaagaccgt cgtgtcctgc
     1561 gtgccggtca cgcgcacgca ggggccgccg gaatccccct tcgagacgta ttgaccgagc
     1621 tcgttgggca gcaccacgtc gtagtaaatg ctcgagttcg gggcacggtg atcgatggcg
     1681 agtgtgaaga caccggtgtt gatgccgtaa ttttcgatgt tgcccgcccc gtagcagcgt
     1741 gcctgttgtc ccgccagttc ctgcggcgtg agattcgaga acgtcactcg agaagtcgtt
     1801 ggctcgggcc gtttgagctc caccaaggcg gcgtccacgg tatcgtctcc tcctgcaagc
     1861 gcgagcggat gctgaatgac gcgcttggct ccgccgcccg gaatcgaacc atcgggacgt
     1921 cggatggcgg gcagcccgtc gtccgtgcag tgccgcgccg tgagcacgta gctcgccgtg
     1981 cccgacgagt taccgagtag aagcccgccg catccactga cataaaccat caggttttcg
     2041 taggggcgcg ccgccgggtc gagcggactg ccattgcgga tctccgactg agtcgcgtcc
     2101 acgtcttcgt tcattccatc agccccggct tgagctgagc agcccaaacc cgagagaccg
     2161 agcgtcaagg ttgctaatgc aagggcggtc gtaaacttcg aattcatatt cgccatatcc
     2221 ttctgtgttc gtcgatcggc tcgaacacga ccgccctcgg agcacatccc gttccagccc
     2281 ctcgaaccct ggagctctcg tcaaaaaggc cttcgccagg cgactcaggc gaaccgtcgg
     2341 ctgtccactc ttgaccgccg accaccgcgc gcagtgacct ccgcggatga gccctacgtc
     2401 tcgcagccca aaactcggat gaagaactcg cgcctcacgc tgtccgcgcc gtgtccgcca
     2461 ctgtccaaac ttgaaattcc gcgagaaaat gactcacgtc aagcggtcct ccgcgaacgc
     2521 atggtcctga ctcgcgacct cgtacaagcc gtacgcttcg aagcccgacg cctccgaaaa
     2581 ccccgagtgg aagacggagt acggagagtg agctctcgac cgcaacctcg cgttgacgcg
     2641 ctctacagca aaccgccgtc tagacggacg gattgggccc gcaacggcta cgcaagcctt
     2701 ctcgctcggc aggtgacaac tcggtcaacg gaaacttcgg acccggagca ttgcgctcga
     2761 agaatggatg cggccaggct ttgccggaga gcttccagcc gagcagcagc cagaccacgc
     2821 gcaggagcga gctcgccttg ggcttcacga ccccctccgg tattccaccg atcgcgcgct
     2881 ccagagccac ggcaccgcgg tttggaccaa aggcttccgt gcgccgctct gggctaggtg
     2941 ccccaaataa cacctcgtga ggcaggccaa cgaacggcag cgccggcgtc cgggtattgc
     3001 cgagtggcgt cttgcagcac gaggcgtacc aacgatgcat gccgccggga gctagtcgca
     3061 cgcccgcgat gagttccgtg ccgcggtcgt aagatatcga atttggcggc acttgcacga
     3121 tctcggtacc gccgtgggcg tccatgagat ccgcacgacc caagtgatga aggaacgctt
     3181 gacagtcgct gcaataacac accatgcgat tcacggtact tggcgcagtc cccttgagcc
     3241 agccatgcac cttgccacat cggcaccgca actccacgtc tccgtccatg accctcccgt
     3301 agcacgcgag ccgcggccct gtcatccggc gcaagcatct gagcctcgcc cccacgtgcc
     3361 ccgccttact gccgcaaccg agtgagtgtc ggctggaaaa ccgcaagaga cggtgaagct
     3421 cggaacaggt ccgagccgtg cgacgttccc tatgccgcat ggctgcctcg gctccgtcga
     3481 cctcagctcc acctcggact tcaaagcaga aacgctcacg ctcggtactg cttgccctcc
     3541 ttgggttcgt gatcctgatt gcgcttggaa tagagctgcg atcccgacac cgactggctc
     3601 actttgccga cctgacagcg cgtgagcgcc gccagctaga ggctgccaaa ccgcgtagcg
     3661 tctacgccga gccgctgccc ggcgacgcgc gacgcgaact ggacgtgatc ctcggcgccg
     3721 ctcgggttcc gagctccgtc aagctccaac cccaagacct cgcgcggcca gagggtctct
     3781 cgtcagctca gcaagcggcg ctggacggac tttcccccgt aatgccgcag gtctcgcgtg
     3841 cactgcacat gaatcaccgc tctggccttc gcgaagcctt ctcgtacaag accgcgtttg
     3901 ccatacacgc cgctctcgca agagccggcg aacgccggct gcctaccgag cccgcggctg
     3961 ggacgcggca aatcctagat gcactcgtgt tcggagccga gctagggcac aacggaagcg
     4021 tgttccccgc gctggtcggg gcgagcctcc tggaagcctc ctgcgcgcag atcgagcggc
     4081 tttggaacca gctcgatccc gagctgcgcc acgcggcact cgcacagacg agcttaccac
     4141 cccctcaagc ctggctgatg ggcgaaaagc tgctccactt ctcgacctta ctagagttga
     4201 gtcaggcgaa ctttggattc gaagacggcc ctaccctgct cgcgccgccg tcacgacgac
     4261 tcgaaatcac caaggcccta ttcgcggcag agaccttttt cgagcaggcc aaccgcgctc
     4321 tttcgcttca accgccgagc agcgaacgct gcgagcaagc gtacctgtcg ctacaacgcg
     4381 aagtgcagct cggctcgctc acgccgacaa acgtatgcag tcaggtgcag aagtttacgc
     4441 gagcccagca agcgctcgag cgcctccgga cccgcgccgg gagcatccgg tagtcccgcg
     4501 cgtgaggggc gcgccgttca acacgccgcg cttcaccgac ttcggcacgc gaccacgcgg
     4561 acaccaacgc atccccaagc gtccaccgag tgtccactcc acggcgagaa cactaaaggc
     4621 ggcgctcgat atagcgtatg ccgagtgtcc agactcgatg tgtggccgga caccgagcac
     4681 gccggaaggc gcccgcatcg cgtctcctcc atgtgatctc ggggaaaacc gagcatcccc
     4741 cgggcccacc ctggcccaga cctttggcac gactggtgca cttatccgat tcgcactcgg
     4801 gctgacccgc agcgttctgc attcacgggt tgacggcata gagccgctcg agactcgcat
     4861 ccgtcggcga gccgccaggc gggctgagaa aggtagctag gaatcgtgat gagcatcaag
     4921 caatgtattc tgggtatttg cgtgggttgg tccgggctcg ctctcgcggg ctgctcgtcg
     4981 ggtggcgcgc tcgagggtgc aagtgccgat ggtggggatg gcgacacggc cgccacggaa
     5041 gaagcactgg gttccgtgtc gtgctcaacc gcgacggccg acaagaccct cgacggctcc
     5101 gccgatccaa ctcacgtttc cccgtccacc tacaacacct gctatcgcgg gtacgtcgtc
     5161 gatatcaaca acttgtcggt atcttatacg ggcgttaagt ccgcgagctc cggctacgac
     5221 gcgcacatct cggtcgactg gaaaggcccc gttcccacca ccaagagcgc ctgcgagtca
     5281 gcgtggggct ccgctatctt ctacaagaag gtgggtggtt cgtgggtcga ccaatccggg
     5341 gtgctgcaag cctacgggga atggcaagat cgcggcgacc tcgtctcctt ctgttctccc
     5401 ccctcgatca cgaccctcga cgtactcacc ctcgaagccg gcgcttccta ccgagtggca
     5461 gcgaccatga gaaccaccta cggtggttca acgctgcgcg cgctcggtgt taccacgcgc
     5521 aagaaggtct ccttctaaca ccagcgcggc gccccgcgta aaccaaccgc tcccatccct
     5581 tgtcggccct gtgcgccggc ctagggaatg ggagcggctc acttcaatcg cagccgcacg
     5641 cgctcaaacc agctcgctcc caagttgcct ttgtctgcgg ccacaatcgg aacgagcgcg
     5701 ccgtgcgaca ctcttcgtca ccagcgcagt ctgaaatgca ctcgcttgcc cactcgcacc
     5761 gcgcaggccc catcaaacaa catcgtcgca tcgagatggg ggagttcgac caatcatggt
     5821 tgcagtggct gtgcgaacct accgcgggat tcgagggctt gattcggtga tgttgtcgag
     5881 ggcgagcaca tcgggcgcgc gcgcggtcgt gacgttgacg gagggggccg gcgcggtggg
     5941 gcgggcactc tggagggctt cgactgcgct tgagacgagt cgtgacgagg gtcgcgctcg
     6001 tctactgagt ggccatctga aaagatgtag tttgaatgtc gtcggccgaa cacgcggagc
     6061 cgtgtcagtg taccccgtcg attaggtgct cagtcgacgg gcgcgatggg atgagaccgc
     6121 aagggcgagc aaggagatga gggcgcctgg tattgcggtg aagaggatga cgggggcacc
     6181 caggcggacg atggtgtcgt cgagggtatg gcgggagtag gtgactgcga gttcggtgcc
     6241 gagtgggaac gcaaggcaag caaggccgat gctgatggcc cagcgatcga gggctcggcc
     6301 gagctgggag tcgaataggt gtgcgctcaa acccgctagg gtgatgaatg gtgtcgcgat
     6361 ggccagcag
//
LOCUS       DASTKU010000796         4117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_824881, whole genome shotgun sequence.
ACCESSION   DASTKU010000796 DASTKU010000000
VERSION     DASTKU010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824881"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFQ61_27830"
     CDS             complement(<1..452)
                     /locus_tag="VFQ61_27830"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET9958349.1"
                     /translation="MSSSASYGSTAPSTPSSNPSPIGFAALAGPLLFAHRGARLELPE
                     NTLPAFERARELGADVLELDVHATRDGHAVVCHDPDLSRTAGLPRRIRDLSLAELREI
                     EVRRPEEPGLRAKVPTLLELLEQLPSAVLNVDVKEARALDPTLEVIRRV"
     gene            complement(531..1268)
                     /locus_tag="VFQ61_27835"
     CDS             complement(531..1268)
                     /locus_tag="VFQ61_27835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958350.1"
                     /translation="MTKNTARMLGALAAALCVAYFYFDKLSHAQCPGAVFVEFHPPLV
                     EAGRYRVRLSFEGQTRPCEFQVPLPLSGTVDTSSCHLNLALKTRSRANETAITGLAVG
                     AAPKALDLKITRDTEDIYDAHILPRYSPYPTRREDDKRFCGPQARAQPTCIANSSGCT
                     PFTTNCDGPEDCPKPRVCCASPEWGREYGAKSATECAPSRRNCLDRLALVACHADTDC
                     PSDMHCAAEPVRREFRTPFAVCQYKTK"
     gene            1558..2019
                     /locus_tag="VFQ61_27840"
     CDS             1558..2019
                     /locus_tag="VFQ61_27840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958351.1"
                     /translation="MDQEDEGEGVVHSDRLSPEVVLTRFQGYLTLALAVRAAQRFERI
                     VHQIDAPIWIVDILGLTGFDPAAMAVGTGWWRSFRSRHGQEVVLISRKSVDRMAAATL
                     GFSAGLRVKVFDSPQEAFAYLGAVMSAWPADGQAAAPAHCTSAPHLIPNSS"
     gene            2094..3110
                     /locus_tag="VFQ61_27845"
     CDS             2094..3110
                     /locus_tag="VFQ61_27845"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958352.1"
                     /translation="MATQCLSLLSAFELPWRSARHQLSFEHAEASEERAAQHGLLVRD
                     ADDPNRQRFRAFVELDCFVYPYTSLPVLGAVGGFNQWLYFLDDQYDDHPETHGDLTGI
                     RRIMQRAYELLAGTSAAPAKTPFEQFTRALRLDLLALAPTGFMPRFLENVHDYLFDGS
                     LEAIAHWARAETLPLEDYIELRALDSGVYPVIDCIEIAGGLRLSADVLRHDSVRQLVR
                     CAVRHVALANDIFSYEKEVLLNGSTMNLVHVVSEREGRTLDSAVGRAVEIVNGFARDF
                     IQAERHLPKLSPAQDLALRVFAHGMHAWMRGNVDFSLQSRRFRSGTSPIRELRESEPL
                     ALLG"
     gene            3254..4108
                     /locus_tag="VFQ61_27850"
     CDS             3254..4108
                     /locus_tag="VFQ61_27850"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HET9958353.1"
                     /translation="MSRVRTVDIDELVDLYDALHADEDSSLDFDGILGLLHAIACAPQ
                     PVDSPVWLASVMPRSATPSESEIDRLRTGLIQQLREVELALAEGSAFIPEPDEAEAWE
                     NFARGFVAGAALDPTWHGDPERWALVSWFAYLAGRRADVPGELLETLDADSNASDIIC
                     DQAPAMIRAIYAEFQASASRQLTPPYSGGVNARPGSGRDTGVQGGLKNAAKKPANTPK
                     PHSSSKQDSASRRQNHSTGGGGTRTPAQPNERLGQARDRRHPAVPSASRSSSRHGRPT
                     SPGKPRGR"
BASE COUNT          714 a         1264 c         1338 g          801 t
ORIGIN      
        1 acacgtcgga tgacctcgag cgtcggatcg agcgcgcgag cttccttcac gtccacgttg
       61 agcaccgcac tcggcagctg ctccaatagc tcgagcaagg tcggcacttt ggcgcgcagg
      121 cccggttctt ctggtcggcg cacttcgatc tcacggagct ccgccaaact caggtctcga
      181 atgcgccgcg gcagccccgc cgttcgcgag agatccgggt cgtggcagac gactgcatga
      241 ccgtcgcgcg tcgcgtggac gtcgagctcg agcacgtcgg cacccagctc ccgggctcgt
      301 tcgaatgctg gcaaggtgtt ctcgggcagt tcgagccggg ccccgcgatg ggcgaacagg
      361 aggggacctg cgagcgccgc gaacccgatt gggctgggat tgctcgatgg agtgcttggt
      421 gcagtcgatc cgtacgacgc tgatgaactc aagacagacc tgcgcggctg gtttacttgg
      481 ttggcgtgct tggttggcgt gcttgggttg gcgtgcttgg gttggcgtgc ttacttggtc
      541 ttgtattggc ataccgcaaa aggggttcgg aattctcgtc gcacgggctc ggcggcgcag
      601 tgcatgtccg acgggcagtc cgtgtccgcg tggcacgcca cgagcgcgag acgatcgagg
      661 caattgcggc ggctcggagc gcactcggtg gctgactttg cgccgtactc gcgtccccac
      721 tccgggctcg cgcagcacac ccgcggcttg gggcagtctt ccgggccgtc gcagttggtc
      781 gtaaacggag tgcacccgct actgttggct atgcaggtgg gctgagcccg tgcttggggg
      841 ccgcaaaagc gcttgtcgtc ctcgcgcctc gtcggatacg gactgtatcg agggaggata
      901 tgagcgtcat agatgtcttc tgtatcccgg gtgatcttca ggtcgagtgc tttgggtgcc
      961 gcgcccaccg ctagcccagt gatcgcggtc tcattggcgc gcgatcgcgt cttcaatgcc
     1021 aggttgagat gacaactgga cgtgtccacg gtgccgctca gaggcagcgg cacctgaaac
     1081 tcgcacggcc tcgtttgacc ctcgaagctc agccgcacac gataacgtcc tgcttcgacc
     1141 agggggggat gaaactccac gaacaccgcg cccggacact gggcgtggct cagcttatcg
     1201 aagtagaagt aggcgacgca cagagctgcg gcgagagctc ccagcatgcg ggccgtgttc
     1261 ttcgtcacgg cgggaaagta tcatcgttgc gggcgagctg tccggctccc gctccgttct
     1321 gcaccgatct cgcgcaagta agcgggtgtg gggacgagtc acgacgaaac ttgggccagc
     1381 ttccacgagc ggccaccggc gtggctgcga ctcacgccgg acatgttcga ggggcaacca
     1441 aatcaagacg ggtgagtcgc gcgccgacac acatgacggg cattcggact ttgtcgagac
     1501 tgaactcaag aagggagacc gcacgccgtt ccaccgcatg tccagggaaa caggcgcatg
     1561 gaccaagagg acgaaggtga gggagtagtt cactcggacc gactctctcc tgaggttgtt
     1621 ctaacccgct tccagggcta tctcacgttg gctctcgcgg tgcgagccgc gcaacgattc
     1681 gaacgcattg tccaccagat cgacgctccg atctggatcg tggacatcct cggcctaacg
     1741 ggtttcgatc cagcggcgat ggcggtgggt acgggctggt ggcgctcctt ccgttcgcgt
     1801 cacgggcaag aggtcgtgct gatttcgcgt aagtcagtag atcgaatggc agcggctacg
     1861 ctgggcttca gcgcaggtct gcgcgtcaaa gtgttcgatt cgcctcagga ggcgttcgcc
     1921 tacttggggg ctgtgatgag cgcatggccg gctgatggcc aggccgctgc gcctgctcac
     1981 tgcaccagtg caccgcacct aattcctaat tcgtcctaat tactgcacgg aatagccggg
     2041 cggtgtagct gactgattcc gagaagcggg ggctaactcg aggaggttat gagatggcga
     2101 ctcaatgtct gtctttgctc tctgcgttcg agctgccgtg gcgctcggcg cgtcaccagc
     2161 tctcgttcga acacgccgag gcttcagagg aacgcgcggc gcaacacggc ctgttggtcc
     2221 gggacgctga cgatccaaac cgtcagcgct ttcgcgcgtt cgtcgagctc gattgtttcg
     2281 tgtacccata cacgtctctg ccggtgctcg gcgccgtggg tggcttcaac caatggttgt
     2341 acttcttgga cgaccagtac gacgatcatc cggaaacgca cggtgatctc acgggtattc
     2401 gccgcatcat gcagagggct tacgagctgc tggcgggaac gtcggccgcg cccgcgaaga
     2461 ccccgttcga gcaatttacg cgcgccctgc gtctcgattt gctcgcgctc gcgccgaccg
     2521 gcttcatgcc ccgttttctc gagaatgtgc acgactatct gttcgacgga tcgctcgaag
     2581 ctatcgcgca ctgggcccga gccgaaaccc tccctctcga agactacatc gagttgagag
     2641 ctttggactc tggcgtctac ccggtcatcg actgcatcga aatcgcgggt gggctgcgtt
     2701 tgtctgcgga tgtcttgcgg cacgactccg tgcgccagtt ggtgcgttgc gcagtccgac
     2761 atgtcgcgct cgccaacgac atcttctcct acgaaaagga ggtgctgctc aacggctcca
     2821 cgatgaacct cgtccacgtg gtgagcgagc gcgaaggtcg gactctggat agtgccgtcg
     2881 ggcgtgcggt cgagatcgtc aacggttttg cgcgcgactt catacaggct gagcgtcacc
     2941 tgcccaagct ctcgccggcg caggacttgg cgttgcgggt gttcgcgcac ggtatgcacg
     3001 cctggatgcg aggtaacgtc gacttctctc ttcagagccg gcgctttcgt tcgggcactt
     3061 caccgatccg cgagttgcgg gaatccgagc cgctggcctt gctgggctga agttgctcgg
     3121 ctgaggattc accgaaagca cgcgctgccg gcgcgcggcc aagcaactcc tcgatgacgg
     3181 ggaacgagga cgagcggcga ggacgagctg gggataccta ggccttttgg acgcttgtcg
     3241 gcggacctca gctgtgagca gagtccggac cgtggatatc gacgagctcg tggaccttta
     3301 cgacgccctc catgcagacg aggactcgtc cctagatttc gacggaatat tgggcctttt
     3361 acacgctatt gcgtgcgccc cacagcccgt tgactcaccg gtttggctgg ccagcgtgat
     3421 gcctcgaagc gcaaccccaa gcgaatcgga gatcgatcgc ttgcgcacgg gcctgattca
     3481 acaactccgg gaagtggagc tggcgctggc cgagggttcc gccttcattc ctgagccgga
     3541 tgaggctgaa gcctgggaaa atttcgcgcg tggcttcgtg gcgggcgcgg cgctcgatcc
     3601 tacgtggcac ggcgacccag agcgatgggc tttggtgagc tggttcgcgt acttggccgg
     3661 tcggcgagcg gacgtgcccg gcgagttgct cgaaactctc gacgcggatt ccaacgcgag
     3721 cgatatcatt tgcgaccagg cacccgcgat gattcgcgcc atctacgccg agttccaggc
     3781 ttccgcttcg cgccaactaa ccccgcccta ctccggcggc gtcaacgcgc ggcccgggag
     3841 cgggcgggac accggcgtgc agggcggact caagaatgca gcgaagaagc cggcaaatac
     3901 tcccaagccg cactcgtcct ccaagcaaga ttcagcgtca cgcaggcaga atcacagtac
     3961 gggcgggggc ggcacgcgga ctcccgcaca gccgaacgag cgtcttggac aggcccgcga
     4021 tcgccggcac cccgccgtgc cgtccgcatc tcgctcttcg tcccgacatg gtcggccgac
     4081 ctcgcccgga aaaccgcgcg gacgctgaga cgaatcg
//
LOCUS       DASTKU010000797         4326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1718874, whole genome shotgun sequence.
ACCESSION   DASTKU010000797 DASTKU010000000
VERSION     DASTKU010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718874"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFQ61_27855"
     CDS             <1..745
                     /locus_tag="VFQ61_27855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HET9958354.1"
                     /translation="GTQCEADLVAKINLDYHLAALQLASGTLSPAQYLAISRDRTRKR
                     SVAQPLAELCNMVNADTDGDLVPNARDACPNTPALTPTKDNGCTDTTLPSAPPASDVS
                     KALGKITLLGSKYCQGAPAPTVPTFLKFAPFLLGWHDLVLAHVGNQPAGCPVYYHIQV
                     ESLDITTNKTKSLSTMFQDTESMDPSTAPTVTFRVAGGSDPNRTNISNEIYGAAYSDP
                     TNIRFVRARAMATSGAGARSDWSQWSSWY"
     gene            779..2956
                     /locus_tag="VFQ61_27860"
     CDS             779..2956
                     /locus_tag="VFQ61_27860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958355.1"
                     /translation="MKSIKNVCSAALVAAVAASTAPASAYQHKGHQLTADLAWQVMVA
                     TQRSDLLTIPDLNVARLDKFPGPGAEPPEWREFILDIDRAIALLSESASGLDVPKASG
                     AKCKEHLGSATAPYPNPGDVTGCRLRELQFSPAKEWFDAGEETCPIEKDYTPGGIYEL
                     AHREPIINGSFRTTFDTKFVGTLLGRLATIPDDDVKETTLWQLNPALSLPGMAASKSL
                     ELGLAVALALPVCIVSLFTGDNCWDKAKELANDVNLAQTLLGSVPGDPDSRMNEVEVG
                     GERLDFIGFWHLMQLKPGSTAAANLSFNDVPGLMWEQGGPLGSLGTIDLVGMAFTDIN
                     AIHLNVPESNGVRRYEVKSGPSQSRSRSDAEWASYPVGHIEMNPLDNLAQAGVSAFTA
                     SNIDLSQLARPLHALGDATVPMHTMGTSGWGHSQFEGAIEGKLREILLQRPTDPAGKT
                     TPYTSAELSTQLEQARRILKDGFRWWKRVRELQRNEPQWGYPIRPFLTELATETANRD
                     FECLNNARGDSDFQVVNTDACVMRSPISSSNYKVDIMRPPIEAAAAANLAFLTTIASF
                     AKPLATTAVTPCTVPAQTCGTGLFFNGKSCFQCNSPDRAFHFAFGCYKTCPVGTQPRE
                     YGNCVVPENGCANGTVSNGAGGCVACGDNKFSEGNTCVSVCSGTKSVLAKNCVSSCPA
                     SLRYRAIIGNTASGAFVYGCASRCPQGQAESSSTPYLCALNPH"
     gene            2984..3412
                     /locus_tag="VFQ61_27865"
     CDS             2984..3412
                     /locus_tag="VFQ61_27865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958356.1"
                     /translation="MSIYWTQFGPNIFIHPLWLTSLFWGLLVATALAVIALKRRWVSE
                     RARKSLAMFLTGYLVAIGVLVAGYVLPCALEFARCVLDGESDLITYGHIRNETLQRTL
                     TGSLAGVGFGVGPWGVRWMAKRVRGGAAEARGRGTEAVMA"
     gene            complement(3668..4111)
                     /locus_tag="VFQ61_27870"
     CDS             complement(3668..4111)
                     /locus_tag="VFQ61_27870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958357.1"
                     /translation="MNTARAILWDIAFVAAAVLPAAGFAAGGSLKTIPRAMLLAMLVA
                     LLATLWAPFRRLRGSGVPNTLYNACLRCFQALSLVLLPVGLFASSMFLLLAIFPPYRQ
                     IQGNSMPVMPIAQAFGALILGGGLGLWIWRTYFRNPQSLWRRMSN"
BASE COUNT          878 a         1471 c         1203 g          774 t
ORIGIN      
        1 tggcactcaa tgtgaggcgg acctcgtcgc caagataaac ctcgattatc acctggccgc
       61 tctgcaattg gcgtcgggca ccttatcgcc cgcgcagtac ctcgccatct cgcgagaccg
      121 cacgcgaaaa cgctcggtcg cgcagcccct cgccgagctt tgcaatatgg tgaatgccga
      181 tacggacggt gatctggtcc cgaacgccag agacgcttgt cccaatactc ccgcgctcac
      241 cccaaccaag gacaacggct gtaccgacac cacgctgccc tccgccccgc ctgcgagcga
      301 cgtgagcaag gctctcggca agatcacgtt gttgggttcc aagtactgtc aaggagcgcc
      361 cgcgcccacc gtacccacgt tcctcaagtt cgcccccttc ctactcggct ggcatgatct
      421 ggtgctcgcc cacgtcggca accaacccgc gggctgtccg gtctattacc acattcaagt
      481 cgagtcactc gacatcacca cgaacaagac gaagagcctc tcgaccatgt tccaggacac
      541 tgagtccatg gacccgagca cggcccccac cgtgactttc agagtggcgg gtggcagtga
      601 tccaaaccgc accaatatct cgaatgagat ttatggcgcg gcgtactcgg acccgacgaa
      661 catccgcttc gtccgcgcgc gggccatggc cacttctggc gcgggcgcac gctccgactg
      721 gagccagtgg tcgagttggt actgagcctc gagtcttccg ttcacaagga ttctgaatat
      781 gaaatcgatc aagaatgtgt gttcggcagc cctcgtcgcc gccgtggccg cgagtacggc
      841 ccccgcttcc gcgtaccaac acaaaggcca tcagctcacc gccgacttgg cctggcaagt
      901 gatggtcgcg acgcagcgct cggatctact caccattccg gacctgaacg tcgcgcggct
      961 cgacaagttt ccgggaccgg gcgcggagcc tcccgaatgg cgcgagttca tcctcgacat
     1021 cgatcgtgcg attgccctac tctccgagtc ggcgagtggc ctcgacgtac cgaaagccag
     1081 tggtgccaag tgcaaagaac acctgggctc tgccaccgcg ccgtatccga acccgggcga
     1141 cgtcaccggc tgcagattgc gtgaactcca attctcgccc gccaaggagt ggttcgatgc
     1201 gggtgaggaa acctgcccga tcgagaaaga ctacacgccc ggcgggatct acgagctcgc
     1261 ccaccgcgag ccaatcatca acggctcatt ccgcaccacg ttcgacacca agttcgtcgg
     1321 cacgctcctc gggcgcctcg ccaccatccc cgacgacgac gtcaaggaga cgaccctgtg
     1381 gcagctcaac ccggcgctct cgctaccggg catggcggcg agcaagagcc tggagctagg
     1441 cctcgcggtc gctttggccc tcccggtctg catcgtctcg ctgttcaccg gcgacaactg
     1501 ctgggacaaa gccaaagagc tcgccaatga cgtcaatctg gctcagaccc tgctcggcag
     1561 tgtccccggt gatccagata gcagaatgaa cgaagtagaa gtcggcggcg agcgtctaga
     1621 cttcattggt ttttggcacc tgatgcagct caagccaggc agcactgcag ccgccaacct
     1681 gagcttcaac gatgttccag gattgatgtg ggaacaaggc ggcccactcg gctcactcgg
     1741 caccatcgac ttagtcggca tggccttcac cgatatcaat gccattcacc tgaatgtccc
     1801 cgaatccaac ggcgtacgcc gttacgaggt gaaatccggc ccttcccagt cacgctctcg
     1861 cagtgacgca gagtgggcgt cttatccggt tggtcacatc gagatgaacc cgctcgacaa
     1921 cctcgcccaa gccggcgtgt ccgccttcac cgcgtccaac atcgacctat cccaactcgc
     1981 tcgccccctg cacgccctcg gcgatgccac tgtccccatg cacaccatgg gcacgagcgg
     2041 ctggggacac agccaattcg aaggcgccat cgaaggcaaa ctccgggaaa ttctcctgca
     2101 acgccccacc gatcccgccg gcaaaaccac gccctacacc tccgcggagc tcagcaccca
     2161 actcgagcaa gcgcgccgca tcctcaaaga cggcttccgc tggtggaagc gcgtgcgcga
     2221 actccagagg aacgaaccgc agtggggata tccgattcgc ccattcctca ctgaactcgc
     2281 cactgaaacc gcgaaccgcg acttcgaatg tttgaacaac gcgcgtggag acagtgactt
     2341 ccaggtcgtc aacaccgacg cctgcgtcat gcgctctcct atctcctcca gcaactacaa
     2401 agtcgatatc atgaggccgc ccatcgaggc cgccgctgcc gccaacctcg cattcctcac
     2461 cacgatcgca tccttcgcga agcccctcgc cacgaccgct gttacccctt gcacggtgcc
     2521 cgcccaaact tgcggaacag ggctcttctt caacggcaaa tcctgctttc aatgcaacag
     2581 ccccgatcga gccttccact tcgcattcgg ctgctacaag acctgcccgg tcggcaccca
     2641 accccgcgag tatggtaact gcgtagtccc tgaaaacggc tgtgccaacg gcaccgtgag
     2701 caatggcgct ggcgggtgcg tagcctgtgg tgacaacaag ttctccgagg gcaacacctg
     2761 cgtctccgtc tgctcgggca ccaagagcgt gctcgccaag aactgcgtct cctcctgccc
     2821 tgcgtcactc cggtaccgag ccatcatcgg caacaccgcc tctggagcat tcgtttacgg
     2881 ctgcgcatcc cgctgccctc agggccaagc cgaaagctcc tctaccccct acctctgcgc
     2941 cctaaacccc cactgaatcg agcctcctca ccgcgagccc gccatgagca tctactggac
     3001 ccagttcggc cccaacatct tcattcaccc cctatggctc acgtcactgt tctggggatt
     3061 gctcgtggca acggctctcg cggtgatcgc actgaagcgg cgctgggtca gcgagcgagc
     3121 acggaaaagc ctcgcaatgt ttctgacggg gtacctagtc gcaattgggg tgctcgtcgc
     3181 ggggtacgta ttgccctgtg ccctcgagtt cgcccgctgc gtgctcgacg gtgagagcga
     3241 cctcatcaca tatgggcata tccgcaacga gaccctgcaa cgtaccctga cgggcagctt
     3301 ggcgggcgtc ggattcggcg tggggccatg gggggtacgt tggatggcga agcgggtacg
     3361 cgggggagcg gcggaggcgc gagggagagg gacggaggcg gtgatggcgt gagagtggac
     3421 gtccatgctg cgcgacgcct tcccctcact cgatagcggc ctcccgggtg tcgccaatcg
     3481 aggtcgacat cctttgagta ctccccatct ggcacccgtg gaactcttga gccccgaccc
     3541 tgagttgtgc gggtgacgat atcgggccat gtccagcttt ctcgggcttc ggcccctatc
     3601 cgcgaccggg gattctcacc tggaccaact ccgactaact cacctacagg caactcgaga
     3661 ccatgcgtca attcgacata cgccgccaca gggattgggg gttgcgaaag taggtgcgcc
     3721 atatccagag gcctagccct ccccctaaga tcaaagcgcc gaatgcctgc gcaatcggca
     3781 tcactggcat gctgttgcct tggatctgtc gatatggcgg gaagatcgcc agaagcagaa
     3841 acattgaact agcaaagaga ccgacgggca gaagcacgag tgacaaagct tggaagcaac
     3901 gcaggcaagc attgtacaga gtattgggca cgccacttcc tcttagccgc cgaaacggcg
     3961 cccatagggt cgcgagcagc gcgaccagca ttgcgaggag catcgcgcga ggaatggttt
     4021 tgagtgaacc gcccgccgca aagcccgcgg caggcagtac cgccgccgcc acgaatgcga
     4081 tgtcccacaa gattgcgcgt gcggtattca tgcctctcaa ccagagtcga tcctacaggc
     4141 gggcagcgac gtacgatctc aacgcacccc caccaaacgc atcgtgccaa gtggtgggcg
     4201 cgccgtcaag gctctcgggc catcctgact tcgagtcgac gtgcccggcg agtctgcgtt
     4261 tcgtcagttg gatgatccta cgctgagtgg gaaagatgtc gcttcgttgg ggcccaccgc
     4321 aagttg
//
LOCUS       DASTKU010000798         5862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_378566, whole genome shotgun sequence.
ACCESSION   DASTKU010000798 DASTKU010000000
VERSION     DASTKU010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378566"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VFQ61_27875"
     CDS             <1..806
                     /locus_tag="VFQ61_27875"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9958358.1"
                     /translation="AWDALRARGPERARVNPERQLIGGVAVVRYVPPGRTPRRVILYL
                     HGGSFIYGSERSHAEIAAGLALAAEAEVILPLYRLAPEHPFPAALEDVITVYHELLAR
                     GVQSTQLVLAGDSAGGNLTLATLLVLRDSHHALPAGAVPISPWLDLEDQSGSMQRHSR
                     FDWAEPWMFQRWAKIYLGPGGDARDPRASPARAELSGLPPCLLVVGTAEMLYDQVCAF
                     AQRARACGVEIELSEWPERVHLWLALKPMFPEFQATFDKVGEFVKRVTA"
     gene            complement(829..2562)
                     /locus_tag="VFQ61_27880"
     CDS             complement(829..2562)
                     /locus_tag="VFQ61_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9958359.1"
                     /translation="MPPGNDSTAKLSTEFFGYEILVTGAGSVSIVPLPENGELSIGRA
                     EDATLRLTSNSVSRRHARLRVHGADLSLEDFGSANGTLLNGERVPAQREIPVQAGQTI
                     LIGDFALVLRTRRAAATTRHIWSSEYFEARIEEQCERSRDAGSVPFHLLKIGVEAAPS
                     GLDVPALIAQQLKSSDLLARLAESVFGVLLLEDSESSAGALGARVERALKQHSCRAGV
                     EHFSFPRDGTSASSLMGRILGSPQTESDLDSARFILRSPAMVALYQQIEPVARTHLNV
                     LILGETGVGKDVLARNIHERSERRDGPFLRLNCAALPEQLLESELFGHEKGAFTGATH
                     QKPGLLVSAAGGSVFLDEIGELPQRLQPKLLQVLETHEILSVGSVKPRRLDVRFIAAS
                     NRDLEVEAAVGDFRSDLYYRLAGFTALIPPLRERREDILPLAREFIRHTAAKLNYQSA
                     PPIRDEAASKLLDYAWPGNVRELRNVVERALVLAGGRPIGPEHLPLERMRSVVLVAPR
                     NRTSGDSQSSAPVLDLTPKELAERERILEALTECAGNQSKAAERLGISRSTLVHRLDA
                     YRIGRPRKRPQ"
     gene            complement(2564..>5862)
                     /locus_tag="VFQ61_27885"
     CDS             complement(2564..>5862)
                     /locus_tag="VFQ61_27885"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9958360.1"
                     /translation="REGEALERLNHPNIVRVLAQVRELDRDYLVMEYIPGGSLETLLR
                     HGEEPLPLGRVLAIALDLCDALTRTHRLDIIHRDLKPSNVLLAEDGSPRLTDFGVAFM
                     EGQERLTESASMVGTPDYLSPEALQGEELDARADLWAFGVLLFEMLTGKRPFGHRNIA
                     QTFHNIAFAPPPAIGTLRPDCPPALADLIYRLLAKNRGDRVPSARQVGAELDRILHGW
                     PSPALVPRSTPLATAGLATTEARPALRHNLPHETATFVGRKPELAELERFFDQAQTRL
                     VTVLAPGGMGKTRLALELCQRWLSAAAVIGPEARSDAVTRRQQVEHGIFFVELAPLAH
                     ANFVVSAISEAVGYPFYAGAEPKQQLIEHLRTKRMLLLLDNFEHVLDAAGLVHDILRS
                     CEGIRILVTSRQRLGLGIETLYPLAGMDFPEPAALDQASDFSAVRLFVQGARQVQPDF
                     RLDSKEAEHVVQICSLVHGVPLGIVLSASWASVLSPSEIAGEIARNMDFLAAELKDLP
                     ERQRSIRAVFDHSWRLLEAPGRDALAALSVFRGGFTRDAARVVAQAGLPALAGLIDRS
                     LLRRDAAQGRYHVHELLREYAGQMLAKDPAREEQVETAHARYFARFLAERETRLKGPQ
                     PVLAAQEIELELDNVRAAYAYWVSAGNFEELEPMLEPMLLFHSRRASFAEAESAYGEL
                     IERVKSIGAPNARLLARALALRALFLRQQGRYAQAEALLVEALAQLESESVDRTSRER
                     ALALVILGSTKAKTGSVQEGAELAERGLALYRETRDTWGLAQALETLGRLHSTAGDFE
                     KAERVYSEGSRIQRASGMLPSALMGLGIATSQQGNYQAGCTLMLEALAMFEKAGDTWN
                     KMRCQMNLANAKRNIGAYAEAESLARDCLNFSCEVSNWDHEAWSLFQLGNILKEQNRY
                     AEAAEYFERARERSLQAGDAGKIALAELEFGDLALIRGELELATRHLTASLEGFESAG
                     QTWGVALALDQLAYAACQAGDHRRARDFFARALETALTRKLLPFALNSAAGLARLLAR
                     SGQDARALELLTMVELHPAVERHTLTRRVAPLRSELEARLPDRTLTEARQNGSRCSLA
                     EIAAFLEQVA"
BASE COUNT         1000 a         1885 c         1914 g         1063 t
ORIGIN      
        1 gagcctggga cgcgttgcgc gcccgtggac cagagcgcgc gcgcgtgaat cccgagcgtc
       61 agctgatcgg gggcgtggca gtagtgcgct acgttccacc cggacgtacc ccgcgccgtg
      121 taattctata tctgcacggg ggcagcttca tctacggttc ggagcggtcg cacgcggaga
      181 tcgcggcggg tttggcgctc gccgccgagg ccgaggtgat cctgccgttg tatcggcttg
      241 ctcccgagca cccattcccc gccgccttgg aggatgtgat cacggtctat cacgagctgc
      301 tcgcacgagg tgtgcagtcg acgcaattgg tgttggcggg cgattccgcg ggcggaaatc
      361 tgaccctggc gactctgctc gtgttgcggg attctcatca cgcgttaccg gccggagcgg
      421 tgccgatctc accttggctg gatctggagg atcaatctgg gtccatgcag cggcactcgc
      481 gattcgattg ggcagagccc tggatgttcc agcgttgggc caagatctac cttggccccg
      541 gcggtgatgc ccgcgatcca cgcgcctcac cggcgagagc agagctgagc ggactacccc
      601 cgtgtctctt ggtggttggc actgccgaga tgctctacga ccaagtctgc gcgttcgcgc
      661 agcgcgctcg cgcttgcggg gtagagatag aactctcgga gtggccagag cgcgtgcacc
      721 tgtggctcgc cctcaagccc atgtttccag agttccaggc cacgttcgac aaagtggggg
      781 agttcgtgaa acgggtgacc gcgtgagccg cgccgatttg cgtggctctc actgcgggcg
      841 tttgcgtggg cggccgatgc ggtaagcatc cagccggtga actagcgtgc tgcgcgagat
      901 gccgagccgc tcggcggctt tcgactggtt tccggcgcac tccgtgagcg cctccaagat
      961 tcgctctcgt tctgctagtt cctttggcgt caagtcgagc acaggcgccg aactctggct
     1021 atctccgctc gtccgattgc ggggcgccac caggacgacc gagcgcattc gctccagggg
     1081 tagatgctcg ggaccaatcg gtctgccacc cgccaatacc agcgcacgtt ctaccacgtt
     1141 gcgcagctca cgtacgttgc ccggccatgc atagtcgagc agcttgctcg cggcctcgtc
     1201 ccgaatcggc ggcgcactct gatagttcag cttggcggcg gtgtgacgaa tgaactcgcg
     1261 cgcgagcggc aggatgtcct cgcgccgttc gcgcagcggt gggattagag ccgtgaagcc
     1321 ggccaggcga tagtacagat cgctccggaa atcccccacg gcagcctcga cctccaagtc
     1381 gcgattggac gcggcgatga agcgtacgtc gaggcgtctt ggcttgacgc tgcctacgct
     1441 caagatctcg tgggtctcca ggacctgcag cagcttgggc tgcaagcgct gaggcaactc
     1501 gccgatttcg tccaagaaga cactgcctcc cgcggccgag accagcaagc ccggtttttg
     1561 gtgggtcgcc ccggtgaagg cacccttctc gtggccgaag agctcactct ccaagagctg
     1621 ctccggcagc gccgcgcaat tcaggcgcaa gaacggccca tcgcgccgct ccgaacgctc
     1681 gtggatgttg cgcgccaaca cgtctttccc gaccccggtt tcgcccagga tcagcacgtt
     1741 caggtgcgtt cgcgcgacag gttcgatctg ttgatagagc gccaccatgg ccggtgagcg
     1801 caggatgaag cgggccgaat cgagatcgga ctcggtctgc ggagagccga gaatgcgccc
     1861 catcagtgag ctggcagagg tgccgtcgcg ggggaagctg aagtgctcga cgccagctcg
     1921 gcagctgtgt tgcttgaggg cgcgctccac ccgagcgccg agcgcgcccg cgctgctctc
     1981 ggagtcttcc agcaaaagaa cgccgaatac actctccgct aggcgagcca gtaaatccga
     2041 actcttgagt tgttgggcga tcagggcggg cacatccaaa ccggacgggg ccgcctcgac
     2101 gccaatcttc agcaagtgaa agggcaccga gcccgcgtcg cggctgcgct cacattgctc
     2161 ctcgatgcga gcctcgaaat actccgagct ccagatgtgg cgggtggtgg cggccgcgcg
     2221 gcgcgtgcgc agcaccaacg cgaaatcacc gatcaagatc gtttgccccg cttggaccgg
     2281 gatttcgcgt tgcgccggga cccgttcgcc attcaagagg gtgccgttgg cggagccgaa
     2341 atcctccaaa ctcagatctg cgccgtgcac cctgagccgg gcatgccggc gagacacgct
     2401 gttggatgtc aaacgcaagg ttgcgtcttc ggctcgtccg atcgagagct ccccgttctc
     2461 cggaaggggc acgatcgaga cactgcccgc gcccgtaacc aggatttcgt agccgaagaa
     2521 ttcagtcgag agtttggcgg tcgagtcgtt accaggtggc attttaggca acttgctcca
     2581 agaatgcggc gatctcggcg aggctgcagc gtgaaccgtt ttggcgagcc tcggtcaggg
     2641 tgcgatcggg cagccgcgcc tcgagttcgc tgcgtagagg tgctacgcgc cgggtgaggg
     2701 tgtggcgctc gactgcaggg tgcagctcta ccatcgtgag cagctcgagt gctcgcgcgt
     2761 cttgcccgct gcgggcgagc aggcgggcca gcccggcggc gctgttcaag gcgaacggca
     2821 ggagcttgcg cgtcagggcg gtctcgagcg cgcgcgcgaa gaagtcgcgg gcacgccggt
     2881 ggtccccagc ttggcatgcg gcatacgcga gctgatctag agccagtgca accccccagg
     2941 tttggccggc gctctcgaag ccctcgaggc tggcggtcaa atgacgcgtg gctagctcga
     3001 gctccccgcg gatgagcgcc agatctccga actcgagctc cgccagcgcg atcttcccgg
     3061 cgtccccagc ttgcaggctc cgttcgcgcg cccgttcgaa gtactccgcg gcctccgcgt
     3121 agcggttctg ttccttcaag atgttgccga gttggaacag cgaccacgct tcgtgatccc
     3181 agttgctcac ttcacaggag aagttcaggc agtctcgcgc gagcgattcg gcttctgcgt
     3241 aggccccgat gttacgcttg gcgttcgcga gattcatctg gcagcgcatc ttgttccatg
     3301 tgtcgccagc tttctcgaac atggcgagcg cctctagcat cagggtgcag ccggcctggt
     3361 agttgccctg ctgggaggtc gcgatgccga gccccatcaa ggccgacggt aacatgccgg
     3421 aggcgcgctg gatgcgggaa ccttcgctat agacgcgctc cgccttctcg aaatcaccgg
     3481 ccgtagagtg cagccgcccg agcgtctcca gggcttgggc gagtccccag gtatcacgcg
     3541 tctcccggta gagggccagg cctcgctccg cgagctccgc accctcctgc acgctcccgg
     3601 tcttcgcctt ggtgcttccc aagatgacga gcgccagggc tcgttctcgg gatgttcgat
     3661 cgacagactc actttcgagc tgagccaagg cctcgaccag cagcgcctcg gcctgcgcgt
     3721 agcggccttg ctgccgcaga aagagcgccc gcagggccag tgcgcgcgcg agcaaacggg
     3781 cattgggcgc gccgatcgat ttcacgcgct cgatcagctc cccgtaggcc gactcggctt
     3841 ctgcgaagga agcacggcga gaatgaaata gcagcatggg ctcgagcatc ggctcgagtt
     3901 cctcgaagtt accggccgag acccagtaag cgtatgccgc gcgtacattg tcgagttcga
     3961 gctcgatctc ctgcgcagcg agaaccggtt ggggcccctt gagccgggtt tctcgctccg
     4021 ccaagaaccg cgcaaaatag cgggcgtgcg cggtctccac ttgctcttca cgcgcaggat
     4081 ctttggccaa catctggccc gcgtattcgc gcagcaactc gtggacgtgg tagcggcctt
     4141 gggccgcgtc ccggcgtagc agcgatcgat cgatcagccc ggccagggcc ggcaggcctg
     4201 cttgggctac gacacgggcc gcgtctcgcg tgaagccacc gcgaaatacg ctcaatgccg
     4261 ccaacgcatc acggcccggc gcttcgagca accgccagga gtggtcgaac acggcgcgga
     4321 tgctgcgctg gcgctccggc aaatccttga gttcggcggc caagaaatcc atgttgcgcg
     4381 cgatctctcc tgctatttcc gagggggaca gcacgcttgc ccacgaggcg gagagcacga
     4441 tgccgagcgg aactccgtgt accaagctgc agatctgaac gacgtgttcc gcctctttgc
     4501 tgtccaaacg aaagtccggt tgcacctgac gtgcgccctg cacgaacaaa cgcacggcgc
     4561 tgaagtcgga ggcctgatcg agcgcggccg gttctggaaa atccatgccc gcgagcggat
     4621 acagggtctc gatgccgagc ccgagccgct gccgcgaagt caccaggatg cgaatgcctt
     4681 cgcagcttct caggatgtcg tgcaccagac cggcggcatc gagcacgtgc tcgaagttgt
     4741 cgagcaagag cagcatccgc ttggtgcgca ggtgttcgat cagttgttgt ttgggttccg
     4801 ccccggcgta gaatgggtac ccgaccgcct ccgaaatcgc cgagaccacg aagttcgcat
     4861 gggccaacgg cgcgagctcc acgaagaaga tgccgtgttc gacctgttgg cgccgcgtga
     4921 ccgcgtccga gcgcgcctcc ggcccgatca cggccgccgc gctcaaccag cgctggcaaa
     4981 gctccagcgc caagcgcgtc ttacccatcc ctcccggggc gagcaccgtg accaggcgcg
     5041 tctgcgcctg atcgaagaag cgctccagtt ccgctagctc gggctttcta cctacgaagg
     5101 tcgcggtttc gtgcggcagg ttatggcgca gcgccgggcg ggcctcggtg gtggcgagcc
     5161 cggcggtggc gagtggcgtc gagcgcggca ccagcgcggg gctcggccaa ccatgtaaga
     5221 tgcgatcgag ctcggcccct acctggcgcg cgctcggcac gcggtcaccg cggtttttgg
     5281 ccagcaatcg gtagatcaga tccgccaacg ccggcgggca gtcagggcgc agcgtgccta
     5341 tcgcgggagg cggcgcaaac gcaatgttgt ggaaggtctg ggcaatattg cgatgcccaa
     5401 aggggcgctt gcccgtcaac atttcgaaca gcagcacccc gaatgcccac aaatccgcgc
     5461 gggcgtcgag ttcttctccc tggagcgcct cggggctcaa gtagtccggc gtgcccacca
     5521 tcgaggccga ctcggtcagt cgctcttgac cttccatgaa cgccactccg aagtccgtga
     5581 gccttggact cccgtcttcg gccaagagca cgttcgaggg cttgagatct cgatggatga
     5641 tgtccaatcg gtgggtgcga gtcagggcgt cgcacaaatc gagcgcgatg gcgagcacgc
     5701 gtccgagcgg tagcggttcc tccccgtgac gaagcagcgt ctcgagtgaa ccgccgggga
     5761 tgtactccat gacgaggtag tcccgatcca gctcgcggac ttgggccagc acgcgcacga
     5821 tgtttggatg attcagccgc tcgagggctt caccttcacg ac
//
LOCUS       DASTKU010000799         3994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_378668, whole genome shotgun sequence.
ACCESSION   DASTKU010000799 DASTKU010000000
VERSION     DASTKU010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378668"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1164
                     /locus_tag="VFQ61_27890"
     CDS             118..1164
                     /locus_tag="VFQ61_27890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9958361.1"
                     /translation="MSEQFSVLAVHPLASSQALSLSSATCHVRAVRSPEQLMDACSTD
                     EYDAVMVVPSELSWCLAELGRAQVRTPVLLALDVPVWPADACAALPRAVNWTQPESWI
                     AALRALRAERVAERREELDARIRRTEKLRLFSELSSNMAHDLNNLVNPLSLFLDLLER
                     EISASNQERALAHVSALRKVAQRCLEHVEGLRRVARPTLQRELDEVSLPAVVEDALLL
                     TQRRLLTAAVKVSREDAGEAVVRAHHEDLVLSVVHLLENAIDAAGPGSTIQIRTGCDA
                     DERWLEIRDSGAGMNEESSRAALEPLFTTKKHGAGLGLNFVQWIAKRYLGTMNLESAP
                     ERGTRVRLSFEAPC"
     gene            complement(1203..2228)
                     /locus_tag="VFQ61_27895"
     CDS             complement(1203..2228)
                     /locus_tag="VFQ61_27895"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET9958362.1"
                     /translation="MVLEIRTPSPVRQLVELSGPATLWVKDDGECIASALDSTHSLIR
                     EAPARRQLYGGNKVRKLAQLMPYAVSGGARRVLTIGTAGSHHALATALLARVYGLESV
                     LFLLPQPFSAHAECMLKANLRHATRVVTVSSPAALAYRMASTLRPNDYVIPPGASNAR
                     GAGAFAQAALELERQVTRGELPEPETIVVALGSGGTAAGLAAGLVASRLRSRVVAVDV
                     VNRGHWTGFWTRNLAARILRSRGLSSARLDQHLEIVEPNYGSYGVPHAAGNEAAARAG
                     RLGLTLEPVYTAKAFAFALTIAQSKGARGPVLYWHTYSACEPPREPAEIPSASAWRDA
                     RRLLVPC"
     gene            2319..>3994
                     /locus_tag="VFQ61_27900"
     CDS             2319..>3994
                     /locus_tag="VFQ61_27900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9958363.1"
                     /translation="MSDRELELEREILRLTQERDEALARAERYRIALDEAPVGVMCVA
                     LREGRYEFCNATHAAILGRELEQVRGGDPYKLWMETTHPDDFPRERLAFQRMIDGEND
                     GYESERRIVQRDGSSKMARVRTRALRDAEGRILYSIVYLQDLSEKLRADLDRERLEAQ
                     LRQAQKLDALGRLAGGVAHDFNNRLVIIMGYSELLKRSLPEDSPLHQHADMILASSAR
                     ASELTRQLLAFSRKQVLKPRTFDVNGCVDGMRRLLSRLIGEHIELVTSLEAAQLVSSD
                     AGQIEQVLINLAINARDAMPRGGRLTLTTRDCELEEGNALSIKPGNYVAISVSDTGTG
                     ISEEVLPRIFEPFFTTKEVGQGTGLGLAMVEGIVEQSGGAVRVESRVGVGSRFTVYLP
                     RAEGGVQAVRSSQLPMAEAPKTDETLLVCDDDDDVRRLVVEVLRMRGGTVLEARNGLE
                     ALELLAPHSKELKLLITDVVMPHMSGVELSRHFRQRCPGLPVLYLSGYTEDYGLLQGA
                     LAPGEHFLAKPFMPGDLTRAAANILDRGVSRSEPPVPLAPTGSSSPVLPD"
BASE COUNT          766 a         1244 c         1282 g          702 t
ORIGIN      
        1 tgtagccggc gggtatgcaa ccagccgcac atcgcaagac ccacggcaag gataccaacg
       61 gcacaggtac caacggcaca gataccgacg cggattgaac cgtaagccag ggtcgatatg
      121 agcgaacagt tcagtgtttt agccgtgcat cccctcgcca gttcgcaagc gctgagcctg
      181 tcgagcgcca cttgtcatgt gcgcgccgtg cgcagtcctg aacagctgat ggatgcgtgc
      241 tcgactgacg aatacgacgc tgtcatggtc gtgccgagcg agctgtcgtg gtgcctcgcg
      301 gagttggggc gcgcccaggt cagaacgccc gtcctgctcg cgctagacgt tcctgtttgg
      361 cccgccgatg cgtgcgccgc attgccgcgg gccgtgaact ggactcaacc agagagctgg
      421 atcgcagcgc tgcgtgcgct gcgcgcggag cgggtcgccg agcgccgcga ggagctcgat
      481 gctcgcattc gccgcaccga gaagctgcgt ctgttttccg agctgtcctc gaacatggcg
      541 catgatctga ataacctcgt aaacccgctg tctttgttcc tcgacctact cgagcgcgaa
      601 atctccgcca gcaaccaaga gcgggcgctc gcgcacgtaa gcgcgcttcg aaaagtagcg
      661 caacgctgct tggagcacgt cgaaggcttg cgccgcgtgg ctcgaccaac gctacagcgc
      721 gaactcgacg aggtttcttt gcccgcggtg gtagaagatg cgctgctgct cacgcaacgt
      781 cggctgctaa cggctgccgt gaaggtgtcc cgggaagacg cgggcgaggc ggtggttcgc
      841 gctcatcacg aagacctcgt cctatccgtc gttcacctgc ttgaaaacgc gatcgacgcg
      901 gcagggcccg gcagcacgat ccaaatcagg accggctgcg acgcagatga gcgttggctc
      961 gaaatccgtg actcgggcgc tggcatgaac gaggagtcga gccgtgccgc gttggaaccg
     1021 cttttcacga ccaagaagca cggcgccggc ctggggctga atttcgtgca atggattgcg
     1081 aaacggtatc tcgggacgat gaatctggag tcggcgccgg agaggggaac tcgagtccgg
     1141 ctttcattcg aggctccctg ttgaactact gcggactcgc actagcagcg cgcaatccag
     1201 gctcaacacg ggaccagtag gcgcctggcg tcgcgccaag cgcttgcgct cggaatctct
     1261 gccggctctc gaggaggctc gcaagccgag taggtatgcc agtacaggac ggggcctcgc
     1321 gcgcctttcg attgagcaat agtcaacgcg aacgcgaagg ctttggcggt gtacacgggc
     1381 tcgagcgtaa gccccaggcg acccgctcgc gcggcagcct cattgcccgc cgcgtgcgga
     1441 acgccgtacg agccgtagtt tggctcgaca atttccaggt gctggtcgag gcgagcagac
     1501 gaaagccccc gcgagcgcag aatccgtgct gccaaattac gagtccaaaa gccagtccag
     1561 tgaccgcgat tcaccacgtc gaccgccacc acgcgcgacc tcagacgaga cgccactagc
     1621 cccgcggcca agcccgcggc ggtccctccc gatcccagcg caaccacgat cgtctctggt
     1681 tcgggtagct cccctcgagt gacctgcctc tcgagttcca aagcggcttg cgcgaaagct
     1741 cccgcgccgc gcgcattgct cgcaccgggc ggaatcacgt aatcgtttgg gcgcagcgtg
     1801 ctggccatcc ggtaagccaa ggcggccggg ctgcttaccg ttacgacacg ggtcgcgtgc
     1861 ctcaggttcg ctttcaacat gcactccgca tgtgccgaga acggctgagg taagagaaac
     1921 aacaccgatt ctagcccgta aacacgcgcg agcagcgccg tcgccaacgc atgatggctt
     1981 cccgccgtac cgatggtaag gaccctgcgc gcgccgccgc tcacggcata cggcatcagc
     2041 tgcgcgagtt tccgcacctt atttccgccg tacaactgcc ggcgcgcggg cgcttcgcgg
     2101 atcagactat gcgtggagtc gagagcgctc gcaatgcact cgccatcgtc cttcacccaa
     2161 agcgtggccg ggcctgataa ctcgacgagt tggcgcacgg ggctcggcgt ccgaatttcg
     2221 agtaccatgg gccagtcttg cggtcggggg agcccggatg caagaccgcg aatgagcgca
     2281 gctgcggaga attcaggcct ctgctactaa attctggcat gagcgaccgt gagctcgagc
     2341 tcgaacgaga gattctccgc ctcacgcagg aacgtgatga agctctcgcg cgtgccgagc
     2401 gctaccgaat cgccttggac gaagcaccgg tgggcgtgat gtgtgtggcg cttcgtgagg
     2461 gtcgctacga gttttgcaac gcgacgcacg cggcgattct gggtcgagag ctcgagcaag
     2521 tacgcggcgg cgatccgtac aagctctgga tggagaccac tcatccggat gacttcccgc
     2581 gcgagaggct ggctttccag cgcatgatcg acggcgaaaa cgacggctac gagagcgagc
     2641 gccggatcgt tcagcgcgac ggcagttcga agatggcgcg cgtaaggacc cgggcgctac
     2701 gcgatgccga agggcgcatt ctctattcga ttgtctattt gcaggatctc tccgaaaagc
     2761 tccgcgcaga tttggaccga gagcgtctgg aggctcagct ccgccaggcc caaaaactcg
     2821 acgctctggg gcgcttggcg ggcggtgttg cccatgattt caacaatcgg ctcgtgatca
     2881 tcatgggcta ctccgagctg ctgaaacggt cgctgcccga ggacagccct ttgcaccagc
     2941 acgccgacat gatcctggcg agctcggcgc gcgcctccga gctcacgcgg cagctgctgg
     3001 cgttcagccg caaacaagtg ctgaagccga gaacgttcga cgtgaacggc tgcgtggatg
     3061 gcatgcgacg ccttctgtcg cggctaattg gtgaacacat cgagctggta acgagcctgg
     3121 aggcggccca actggtcagc tcggatgccg ggcaaatcga gcaagtgcta atcaacctcg
     3181 cgatcaacgc gcgagacgcg atgccgcgtg gcggacgact aacgctgacg acacgagatt
     3241 gtgagctgga ggagggcaat gcgttgtcca tcaagcccgg caactacgtc gcgatcagcg
     3301 tcagcgacac cggcaccgga atttccgagg aggtgttgcc gcgcattttc gagccctttt
     3361 tcaccaccaa ggaggtggga caaggcaccg gcttggggct cgcgatggtg gagggcatcg
     3421 tggagcaaag cggaggcgcc gtccgcgtgg agagccgggt cggagtcggc tcgcgtttta
     3481 cggtgtactt gccacgcgcg gaaggtggcg tgcaagccgt acgcagctcg cagctcccga
     3541 tggcggaagc tccgaaaacc gacgagacgc tgctggtgtg cgatgacgac gacgacgtac
     3601 gaaggctcgt cgtcgaagtt ttgcgcatgc gtggcggaac ggtgctcgag gcgcgcaacg
     3661 gtctcgaggc tttggagctg ctcgcgccac actccaagga actgaagctc ctaatcacgg
     3721 acgtcgtcat gccgcatatg agcggagtgg agttgtcgcg gcactttcgg caacgctgcc
     3781 ccggcctgcc ggttctatat ttgtctggct acaccgaaga ctacggcttg ttgcagggcg
     3841 cgctcgctcc cggagagcac tttctcgcaa agcccttcat gcctggtgac ttgacgcgag
     3901 ccgccgccaa cattctggat cgcggagtgt cacgcagcga gccgccggtt cccctggcgc
     3961 caaccggctc gagctccccc gttctgccgg acca
//
LOCUS       DASTKU010000800         3794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_379045, whole genome shotgun sequence.
ACCESSION   DASTKU010000800 DASTKU010000000
VERSION     DASTKU010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379045"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..697
                     /locus_tag="VFQ61_27905"
     CDS             203..697
                     /locus_tag="VFQ61_27905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958364.1"
                     /translation="MQRLSLRKWVTGIEEQDSQATLRRERHSRHEHSGDDALLPTPSQ
                     KSENLLETADELTEADAQDVERASGVNPRISRPEILEEEEFFADAPPFELTAFIGKRW
                     GLNQDASSQLLTELVGKYVPSSCYEISVLNPQRQESSPREPRDAQPRETLLRDTDDDL
                     AIAV"
     gene            871..1644
                     /locus_tag="VFQ61_27910"
     CDS             871..1644
                     /locus_tag="VFQ61_27910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958365.1"
                     /translation="MDAVRRESDIEASHDTKASVSSEPTSDSPPVPTPDEERNGGPPT
                     TRRHEEGFVELPVPGFLSAVTYLPRREAIALVVATHGAGGSPEWECEYWYRLSRGGMQ
                     LLCLRGTSLGRNYPGSFYYENHLRLEAEFKAALRAFEGGTETTPKVLIYAGFSQGAIM
                     GAPMIVAHASRFSRLVLIEGGFEYWSRGTARKFAKNGGKKVLFLCGTGYCNSRSRVPA
                     DWLASEGVDVRLEHVTGAGHTPAGEVMQRLEAQLPWIVE"
     gene            complement(1689..3122)
                     /locus_tag="VFQ61_27915"
     CDS             complement(1689..3122)
                     /locus_tag="VFQ61_27915"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9958366.1"
                     /translation="MAQQERAFTDTTRVSLREHLTEPAPLESAPTSAELAEAAMAKAV
                     LSEAGTPRVSGTVDIYDANLPGEGEIVGDCYRIIAEAGRGGMGVVLQAFDERLNRRVA
                     IKLLHSQLRCESLRERFLTEARTMALVSHPNVASIFAFGEHRGVPYFVMEYVQGSSVE
                     DWLSAQESPPDIQTVLAILDDVCQGVAAIHEAGAIHRDIKPSNLLLDAEGHVRVADFG
                     LAQPMTELELAREVAGTVAYMAPELVFGENGGGASPLSDVYALGCLAYELLTGRLPFD
                     AALDVSIMVQHATVTAPLPSQVRPELTTAFDGAILHALAKKPAERTPSVEEFRRELRE
                     ARQRFLEPIRILVAEDDEDFRDLLCRKLQAEFPGAVVQCVHDGGEAVRAFDKEPASVV
                     LLDMQMPNLDGVDVTAVLRTRESARQVPIVVMTASGGPAEWKLMSALGADRFLVKPVN
                     LDDLVLSVRSSMKERRASEGGHHRRHK"
BASE COUNT          721 a         1218 c         1132 g          723 t
ORIGIN      
        1 ctgcgggagt gcgaacagtt ggctcatgcc gacgaggggg agccctgcgc tcaaagcccg
       61 aacgggtggt ccggtcgtct ctgcgcaact acgatctcgg cttggtgcaa gtgttgcgcg
      121 catgtatccg cgtctattgc ggaaaaccgg caaatgagcc ctgacaccgt gactggcacg
      181 catcctgcta gcgaattccg acatgcagcg cttgtccctc cgaaagtggg taactggaat
      241 cgaagagcag gattctcagg caactctaag acgtgaacgc cacagccgac atgagcacag
      301 tggcgacgac gctctcctac ccacaccttc acagaagagt gaaaaccttc tcgaaacagc
      361 ggatgaactg accgaagccg acgcccaaga cgtggagcga gcttcgggcg tcaacccacg
      421 tatctcccga ccggagatcc tcgaggagga agagttcttt gctgacgcac ctccgttcga
      481 gctgaccgcg ttcattggca aacgctgggg cctgaatcag gacgcgagca gccagctcct
      541 caccgagctc gtgggcaagt atgtgccgag ctcttgctac gagatctcgg tcttgaaccc
      601 gcaacgtcag gaatcctcgc cgcgagagcc gcgggacgcg cagccgcgag agacgctgct
      661 gcgcgacacg gacgacgacc tcgcgatcgc ggtttgattg acgtttagtc acccaaggct
      721 cgtgtcgcgc ccactttggc aacactcatc gggatgggat ttcggcggag gctagagtgc
      781 ctcgcctttt ccgcgctgta ttgcgggtgc ttttcccatc cgcgaaaccc tcctcctgtt
      841 gccgaagtcg tttcagtaaa tgccaattcc gtggacgctg tccgtcggga atccgacata
      901 gaagcctcac acgacaccaa agcctcagtc agttcggagc ctacgtccga ctctcctccg
      961 gtcccaacgc ctgatgaaga gcgcaatggt ggtccgccca cgacccgccg acacgaagag
     1021 ggcttcgtcg agctccccgt gccgggtttc ttgtctgcag ttacatacct gcctcgccgc
     1081 gaagcgatcg cactcgtggt cgcgacccac ggcgcgggcg gctcacccga gtgggagtgt
     1141 gagtactggt atcggctcag ccgcggcgga atgcagctct tgtgtctgcg tggaactagc
     1201 ctaggccgca attatccagg ttctttttat tacgagaacc atctgcggct ggaggctgag
     1261 ttcaaagcgg cacttcgagc cttcgaaggg ggcaccgaaa ccactcctaa ggtgttgatc
     1321 tacgcgggct tctcacaggg cgcgatcatg ggggcaccga tgatcgttgc ccacgcctcc
     1381 cgcttctctc gcttggtgtt gatcgagggg ggattcgagt actggagccg cggaaccgca
     1441 cgcaagttcg ccaagaatgg aggcaagaag gtgctcttcc tgtgcggcac ggggtactgc
     1501 aactcgcgct cgagagtgcc ggcggattgg ctggcttcgg agggggtgga cgtgcggctc
     1561 gaacacgtaa ccggcgcggg ccatacgcct gcgggcgagg tcatgcagcg cctggaggcg
     1621 caactcccct ggattgtcga gtgagagttg gcgcctccgc gagtgagccg attggaagat
     1681 ggctcttctc acttgtgtcg gcgatggtgc ccgccctcgg acgcgcgtcg ctctttcatg
     1741 gacgagcgca cgctcaacac gagatcgtcg aggttgacgg gtttgaccag gaatcggtcg
     1801 gcgccgagcg cggacattag cttccattcc gccggaccgc ccgacgccgt catcaccacg
     1861 atcggcactt ggcgcgcgct ctcgcgtgta cgcaagaccg ccgtgacgtc cactccatct
     1921 agattcggca tctgcatatc gagcaacacc acggaggcgg gctccttgtc gaacgcgcgc
     1981 acggcttcgc ccccgtcgtg aacgcactgg accaccgcac ccggaaactc tgcctgcagc
     2041 ttgcggcaca ggagatctcg aaagtcctcg tcgtcctcgg ccaccagaat acgaatcggt
     2101 tcgagaaaac gctgacgcgc ctcccgcagc tcgcgccgaa actcctccac ggatggggtg
     2161 cgctccgccg gcttctttgc cagcgcatgg aggatcgctc cgtcgaacgc agtcgtgagc
     2221 tccggacgca cttggctcgg cagcggagcc gtgacggtcg cgtgctggac catgatcgag
     2281 acgtccaacg ccgcatcgaa gggcagacgc cccgtgagca gctcgtacgc gaggcagccc
     2341 aaagcgtaca catcgctcag cgggctggca ccacctccgt tttcaccgaa cacgagctcc
     2401 ggtgccatgt acgccaccgt gcccgctact tcgcgagcca gctcgagctc ggtcatcggc
     2461 tgcgccaacc cgaagtcagc cacgcgaacg tggccttccg cgtccaggag tagattgctc
     2521 ggcttgatgt cgcggtggat tgcgcccgct tcgtggatcg cggcgacgcc ctggcaaacg
     2581 tcatccaaga tcgcgagtac ggtctgaatg tcaggtggcg actcttgggc gctcaaccaa
     2641 tcctcgacgc tcgaaccctg cacgtactcc atcacgaagt acggaacgcc tcggtgttcc
     2701 ccgaatgcga agatactggc aacgttcggg tgacttacca gcgccatcgt ccgcgcctcg
     2761 gtcaagaaac gctcccgcag tgattcgcag cgcagctgcg agtgcaatag tttgatggct
     2821 actcgacggt tcaagcgttc gtcgaaagcc tgcagcacca cgcccatgcc tcctcggcca
     2881 gcctctgcga tgatccgata acaatctccg acaatctcgc cttcgccggg taagttggcg
     2941 tcgtaaatat ccacggttcc actgacgcgg ggggttcccg cttccgacaa cacggccttt
     3001 gccatcgccg cttccgctag ctccgccgag gtgggcgccg actcgagggg tgccggctcg
     3061 gtcagatgct ctcgcagcga aacgcgggta gtgtcggtga atgcacgttc ctgttgagcc
     3121 acagtagccg ctaaagcagc aaggaacatg cccgctgcgc tctactgaat cgccagttac
     3181 ccgcccgaga atggcgtccg cgaacggcac gtttcggtta ctgcaatgag ggccgagaga
     3241 gcgccgggac gcacggtggc aaaccccgct cgcaacaccg gcctaccggc aagacacgtt
     3301 taatttcgaa gacctgcggc cttcactcct gatcagcacg gggttgaatt ccgacccgct
     3361 cctcttctag agagtgcctg ttggtgtcta tccacatcag cggcccgtct atccgcaact
     3421 taccttggga agctcccccc gccgggttgc acaaacgtgc tctggcgtca ctctcggaac
     3481 ccaattttgg ggtggccccc catcctatgc gccgcccgca tcttcaacgg cgccgtcgcg
     3541 ccctggaagg ctggcttcgt tggtgttttt cgcgccgatt atcgccaatg ggcggcacca
     3601 gcttcagtga ggaagcaggg cagatgggct agtttggcac atcgatcccg accaaatcac
     3661 gtggctagat gcggcgggca aaccagcccc gcgcagctat gcctatgatc cccgtgtcgt
     3721 gcgtatcgaa gatatacact gcattctttg gtgcgacgat tcccgggagc ttcgatgggc
     3781 caggccgccc ctgg
//
LOCUS       DASTKU010000801         3152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_825823, whole genome shotgun sequence.
ACCESSION   DASTKU010000801 DASTKU010000000
VERSION     DASTKU010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825823"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFQ61_27920"
     CDS             complement(<1..531)
                     /locus_tag="VFQ61_27920"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET9958367.1"
                     /translation="MLTESAVGVGESAQAQRSRSRHSRFDALVVGGSTGGIQALNELL
                     PPLTRELEVAVVIAVHVPARVPSLLVPIYERRCSLPVREALDKQPVSPCTIWFAPPDY
                     HLMIERTHTFALSLDEPVNFSRPSIDVLFESAAWAYGPRVLGVILSGASRDGSQGALT
                     LRERGGVLAVQDPACSE"
     gene            complement(525..1349)
                     /locus_tag="VFQ61_27925"
     CDS             complement(525..1349)
                     /locus_tag="VFQ61_27925"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET9958368.1"
                     /translation="MNLESFPEDADIERFTELVYRHFNYDFRNYARASLRRRLARAML
                     QFGFARLAELEALLLETPEAFGQVLQYLTVQVSDMFRDPNFFRAFREHVVPELGTYPS
                     PRLWVAGCATGEEVLSIAIVLREEGLLERCLIYATDISPEALRKAQLGVYALDRVAAF
                     TENHQKTGAACSLSAYYTAAYGGVAFDRSLTKRVVFSDHSLATDHVFAEVQVVLCRNV
                     LIYFDKTLQARALGLFTDALSRRGFLGLGSKETLAFTGHAQAYRRVVPDEPWYRRC"
     gene            complement(1346..>3152)
                     /locus_tag="VFQ61_27930"
     CDS             complement(1346..>3152)
                     /locus_tag="VFQ61_27930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9958369.1"
                     /translation="SKIEAGRLDVHLSTTPLARLVEPVTATFAPILEQRKIPLEVRLA
                     ADLPGQIETDVQRVQQILRNLISNAAKFTEQGRITLEVVGRAESLEFRVIDTGIGIAK
                     DQHELIFEAFRQADGTTSRKYGGTGLGLSISRDLARLLGGDLSVQSEPGRGSTFTLTL
                     PASYRGPRPGVTPEHVVTRDAAMVPALTPPRIRELNVAPIVQDDRAALDGKHRVLLVV
                     EDDPAFAQILIDLAHELEFQCVVAGAAEEGLRLARELTPAAVILDVNLPDRSGLSVLD
                     RLKQTPGTRHIPVHVVSVADYSAQALSMGAIGYMLKPVKREQLTAALHKMEERMTRRM
                     SRVLVVEDDPVQRDALCSLLAADDVEAVAVATVAEALAQLRTQSFDCTVSDLSLPDAS
                     GFDFLEQLAADTEHAFPPVIVYTGRALSEDEEQRLRRYSSSIIVKGARSPERLLDEVT
                     LFLHRVEAELPADRRRMLAQVRDREKAFEGRVILVVEDDVRNVFALTSLLEPRGAKVL
                     IARNGRQALELLGQAERVDLVLMDIMMPEMDGLEATREIRKRREWRDLPVIALTAKAM
                     KDDQRACLQAGANDYIPKPLDVDTLLSVLRVWMRK"
BASE COUNT          599 a         1014 c          984 g          555 t
ORIGIN      
        1 ctccgaacaa gcagggtctt gcaccgcgag cacccctccg cgttcgcgca aggttagcgc
       61 gccttggctg ccgtcccggc tcgccccgga gagaatcacc ccaaggacgc gcggaccgta
      121 ggcccaggcc gcagattcga acaacacgtc aatggatggc cgggagaaat tgactggctc
      181 atcgagtgac agagcgaagg tatgggtgcg ttcaatcatc agatgataat cgggaggggc
      241 gaaccaaatc gtgcacggcg aaaccggctg tttgtcgagc gcctcacgca ccgggagact
      301 acaccgtctc tcgtaaatcg gcaccagtag actcggcacg cgcgcgggga cgtgtaccgc
      361 gatcaccacc gcgacttcga gctctcgtgt cagaggcggc aacagctcat tcaaagcctg
      421 aatgccgccg gtagagccgc cgaccaccag cgcatcgaag cgcgagtgac gagacctcga
      481 gcgctgggct tgcgccgatt cacccactcc aactgccgac tccgtcaaca tctccgatac
      541 catggctcgt cgggcacgac gcgccgatag gcttgggcgt ggcccgtgaa cgcgagcgtc
      601 tctttgctgc cgagccccaa aaagcctcgt cggctcaggg catcggtgaa gagacccagg
      661 gcacgcgcct gcaaggtctt gtcgaagtag atgagcacgt tccggcacaa cacgacttgg
      721 acttccgcga acacgtgatc ggttgccagg ctgtggtcgg agaatacgac cctcttggtc
      781 aggctgcggt cgaaggccac gcctccgtaa gctgccgtgt agtacgcgga tagtgagcag
      841 gcggcacccg tcttctggtg gttctcggtg aacgcggcca cgcgatccaa cgcgtagacc
      901 ccgagctgag ccttgcgtag agcctccggg ctgatgtcgg tcgcatagat cagacagcgc
      961 tctagcagcc cttcttcccg cagcacgatg gcgatcgaca acacctcctc gccggtcgcg
     1021 cagcccgcga cccaaagccg cggagatgga taggtaccga gttcgggcac gacgtgctcg
     1081 cgaaatgccc ggaagaaatt cgggtcccgg aacatgtcgc tgacttgaac cgtcaggtac
     1141 tgaagtactt ggccgaaggc ttccggagtt tccagcaaga gcgcctccag ctcggccagg
     1201 cgcgcgaagc caaactgcaa catcgcccgt gccaaacgcc gtctcaagga cgcgcgggcg
     1261 tagttacgaa agtcgtagtt gaaatggcgg tacacgagct cggtgaatcg ctcgatgtcc
     1321 gcatcctccg ggaaggactc gaggttcatt tccgcatcca gaccctcagg accgacagca
     1381 gagtatctac gtcgagtggc ttagggatgt aatcattggc tcccgcttgc aggcatgcac
     1441 gctggtcgtc tttcatggcc ttggctgtca gcgcgatcac gggcaaatcg cgccattcac
     1501 gacgcttacg gatctcgcga gtggcctcca ggccgtccat ctccggcatc atgatgtcca
     1561 tcaacaccag gtccacgcgc tcggcttgcc ccagtagctc gagcgcctgc cgcccgttgc
     1621 gagcgatcaa taccttggct ccgcgtggct cgagcaagct ggtcagagcg aacacgttcc
     1681 gcacgtcgtc ttccacgacc aggatgactc ggccctcgaa cgccttctcg cgatcacgga
     1741 cttgcgccaa catgcgccgt cgatccgccg gcagctcggc ttctactcga tgcaagaaga
     1801 gggtgacttc gtccagcagg cgctccggcg agcgggcccc tttgacgatg atggaactcg
     1861 agtagcggcg cagccgttgc tcttcgtcct cgctgagcgc tcgcccggtg tacacaatca
     1921 cgggtgggaa ggcgtgttcg gtatcggccg cgagctgctc caagaagtcg aagccggacg
     1981 catcgggcaa ggacaagtcc gagacggtac agtcgaagct ctgcgtacgc agctgtgcta
     2041 gcgcctcggc aaccgtcgcg acggccacgg cctccacgtc gtctgccgca agtagggaac
     2101 agagcgcgtc gcgttgcacc gggtcgtcct ctacgacgag gacgcggctc attcgcctgg
     2161 tcatacgctc ttccatcttg tgcagggcgg ccgtcaactg ctcgcgcttg acgggcttga
     2221 gcatgtaccc gatcgcgccc atggacaggg cctgcgccga gtagtcggca acggagacta
     2281 cgtgcacggg gatgtgccgc gtacccggag tttgcttgag gcgatcgaga acggacaggc
     2341 cggagcgatc cggcaagttc acgtccaaga tcacggccgc gggcgtgagc tcgcgcgcga
     2401 ggcgcaaacc ttcttctgcc gcccccgcga ccacacactg aaactcgagt tcgtgggcca
     2461 ggtcgatcag aatctgggca aatgccgggt cgtcttcgac caccaacaac acgcggtgtt
     2521 tgccgtccaa tgcagcccga tcgtcttgta ctatgggggc cacgttgagc tcgcgaatcc
     2581 gtggcggcgt gagcgccgga accatcgctg cgtcgcgcgt gacgacgtgc tccggagtca
     2641 cgcccgggcg aggcccgcgg taactggccg gcaacgtcaa agtaaaggtg ctgccgcgcc
     2701 cgggctcact ttgcacggac aagtcccctc cgagtagccg cgccaaatcc cgcgagatcg
     2761 acaggccgag cccggttccc ccgtatttgc ggctggtggt accgtctgcc tgccggaagg
     2821 cttcgaagat gagctcatgc tggtccttgg caatgccaat cccggtgtcg atcacgcgaa
     2881 attccaggct ctcggcgcgt cccactacct cgagcgtaat gcggccttgc tcggtgaact
     2941 tggctgcgtt cgagatcagg ttgcggagga tctgttgaac gcgctgcaca tcggtctcga
     3001 tctgcccggg caagtcggcc gcgagccgca cctcgagcgg gatcttgcgc tgctcgagga
     3061 tgggcgcgaa ggtggccgtg accggctcga ccaagcgcgc gaggggggtc gtgctcaggt
     3121 gcacgtccaa gcgccccgcc tcgatcttgg ac
//
LOCUS       DASTKU010000802         4892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1498691, whole genome shotgun sequence.
ACCESSION   DASTKU010000802 DASTKU010000000
VERSION     DASTKU010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498691"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1471)
                     /locus_tag="VFQ61_27935"
     CDS             complement(122..1471)
                     /locus_tag="VFQ61_27935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958370.1"
                     /translation="MKKRVFLAAVCAAAALPLEAHAQQWLADRQLQEGIGVRVGDLEL
                     HPGLGGEFGYDSNYFQRAGDGGNPPEPTIAAYRLRIVPSLSLSTLGAQRAGANAPIQF
                     PKVNFRANAHAAYNELFAAESKYSDDVSGQRHLNVGAGFNLDILPHRPVGADLYADYV
                     RLAEPSNSPDQDFAFDRDAIRAGAGVSWRPGGGLFEWRFGYEALYNFFEVERFKTNNN
                     INHTFATRGRWRFLPRTALLFDGSYRFTRYTGPEPTTPTVYDGDWVRSRVGLNGLITP
                     RVSLLAMVGWASSFFDAARRNADTVIAQAEAKYFVLAPPGLDQTSQATTGLSTVWLGY
                     VRDAQNSYLNAYYTRDRAYIGANYFLGGAFVTSVEAGWSYVHFPVNTVASFNQRRIDA
                     RLFAEYRFSNSFGLNATVRFDRNDSDTVRPLAGQMVALADDLDFIRWQAYLGVRWFL"
     gene            complement(1687..2250)
                     /locus_tag="VFQ61_27940"
     CDS             complement(1687..2250)
                     /locus_tag="VFQ61_27940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958371.1"
                     /translation="MGLVALLGIGGVAAAQTAATNSDTPPANTPAVELGGGRDVSLSP
                     QQMVGQARGFLPEMDRSAAVVRRQLGDAREKRDVVRVLCLNDKLNQIDLATRTASDRM
                     DALNAAANQGDSDRTKHEFTVVQVLRERVKTLVSEANQCIGEETGFVGESKVTVTVDP
                     NIPDTDPSVPPEEPLVSPPPLLSSPAL"
     gene            complement(2993..4408)
                     /locus_tag="VFQ61_27945"
     CDS             complement(2993..4408)
                     /locus_tag="VFQ61_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9958372.1"
                     /translation="MTQPSQHSGTGPSQRPGQMTAVMRAVAATGPKVLRIGVVQNGRV
                     TEERIVKQRGHVTIGPSEKNLFVITSDQIPSSFRLFEIVGNDYHLNFLDGMQGRIALP
                     TGISDMNVLKGQARRTSQGAYQIRLTEDSRGKVVIGDTTFLFQFVAPPPMQPKPQLPV
                     AVLRGAGAIDWNSTIIAAFSFLFHFVLLGAIYSDWLDPIVDDEVAVAGLIDSLKNLPP
                     PPPVEDKPEEKEEAKAEEKAEVKAEPKPAGGGPKGPAPGAGALSQREAAAVSNQLEQL
                     EMATLGALSASGPATAGVLKGGEVATGALDQAAASGAGVGVGSGDLRLGGGGGALRPG
                     AVGGGLAGIGSTGRGTGTSGSGTAAAVAGPKGNANVGGAAVSGGSVSNAARVVAGMRA
                     GFRACYQRGLNENPDASGSIRLTIQVGPGGEVSNVTAVPSGSLPPSVVSCVQARARAA
                     QFDRPEGGAATIQVPVQFLKQ"
     gene            complement(4666..>4892)
                     /locus_tag="VFQ61_27950"
     CDS             complement(4666..>4892)
                     /locus_tag="VFQ61_27950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958373.1"
                     /translation="PAALVTFAAGVAGALGVGLVGRSAQARARSAREHFAKVGRKLRL
                     ELRPDIGEGPRDPRPSSSAESASTLGRGPA"
BASE COUNT          901 a         1629 c         1456 g          906 t
ORIGIN      
        1 gtcggcggag ggagattgac cctgcgcgcc gcttgatcgg agccacaccc tgcgccgagc
       61 accagcgccg cacacagcaa gctgcgtcgg gagatgattc gttcgcggag accgcgatgc
      121 tttacaggaa ccagcgaacg cccagatatg cttgccatcg aatgaagtcg agatcgtcgg
      181 ccagggctac catctgcccg gcgagcggcc gcaccgtgtc actgtcgtta cgatcgaagc
      241 gcaccgtggc gttcaatcca aacgagttgc tgaagcgata ttcggcgaac agccgcgcgt
      301 cgatgcgccg ctgattgaag gacgccactg tattgaccgg gaaatgcacg taagaccaac
      361 ccgcttccac ggaggtcacg aaggcgcccc ccaagaaata gttggcgcca atataggctc
      421 ggtcacgcgt gtagtaagcg ttgaggtagc tgttctgggc gtcccgcacg tagcccagcc
      481 aaaccgtcga gagccccgtc gtggcttgac tcgtctggtc gagaccgggc ggtgcaagca
      541 cgaaatactt ggcctcggcc tgcgcgatca cggtgtccgc gttccggcgc gccgcatcga
      601 agaacgaaga ggcccaaccc accatcgcca gcagcgacac gcgcggggtg atcaagccgt
      661 tcaggccgac acgcgagcga acccaatctc catcgtaaac cgtgggagtg gtcggttccg
      721 ggcccgtgta gcgcgtgaag cgatagctcc catcgaacaa cagtgccgtt cgcggcaaga
      781 agcgccagcg gccgcgcgtc gcgaaggtgt gattgatgtt gttgttggtc ttgaagcgct
      841 ccacctcgaa gaagttgtag agagcctcgt atccgaaccg ccactcgaac aagcccccgc
      901 ccgggcgcca ggacacaccc gcgccggcac gaatcgcgtc tcgatcgaag gcgaaatcct
      961 ggtcagggct gttcgacggc tccgctagac gcacgtagtc ggcatagaga tccgcgccca
     1021 cggggcgatg cggcaagata tcgagattga aaccggctcc aacgttgagg tgtcgctgac
     1081 ccgagacatc gtccgagtac ttcgattcgg cagcgaatag ctcgttataa gccgcgtgcg
     1141 cattggcgcg gaaattcacc ttggggaact gaataggcgc gttcgctccg gcacgttgcg
     1201 cccctagcgt cgataacgac agagacggca ctatccgcag gcgatacgcc gcgatcgtcg
     1261 gttccggtgg gtttccccca tcgcccgcgc gctggaagta gttgctgtcg taaccgaact
     1321 caccgcccaa gcccgggtgc agctcgagat ccccgacacg aacaccgatg ccctcttgga
     1381 gttgcctatc ggctagccac tgttgagcgt gagcctcgag gggcaacgcg gcggcagcgc
     1441 agaccgccgc aagaaagaca cgctttttca tctttgactc gatccgctcc gttcagcaag
     1501 ttcagcaaag tcgacctgct cgccgcgctc acgcttccct cgaaatctcg gacgtccctc
     1561 ggtaccgttg cgacccgttt acgtcggcat cgtttcggta ctcagtacgc ccagggtttc
     1621 ctctcaaggc ctcccagcca gcttcttccc agcgtttgcg aagtatctcg cgtattgcga
     1681 tggcactcac aaagctgggc tgctcagcag aggcggcgga ctaaccaggg gttcttcggg
     1741 aggaaccgag ggatcggtgt ccgggatatt tggatccacg gtcaccgtca ccttcgactc
     1801 acccacgaag cccgtctctt ctccgataca ttggttggcc tcgctgacca acgtcttcac
     1861 gcgctcgcgc agcacctgca cgaccgtgaa ttcatgtttc gtgcgatcgg agtcgccttg
     1921 gttcgcagct gcgttcaagg cgtccattcg atcgctcgcc gtgcgcgtag ccaggtcgat
     1981 ttgattgagc ttgtcattca agcaaagtac gcgaacgacg tcacgttttt ctcgcgcatc
     2041 acccagctgg cgcctcacca cggccgcgct gcggtccatc tccggcaaga agcctcgcgc
     2101 ctgaccgacc atctgctgcg gcgacagact cacgtcgcgg ccgcccccga gctccactgc
     2161 aggtgtgttc gcgggcgggg tgtcagaatt cgtcgcggct gtctgcgcgg cggcgacacc
     2221 gccgattcca agcagcgcga ccaatcccat cgccaccgtc gttttggtgg tgcgcagtgt
     2281 ggtggcggct cttgccggca gagttggcga tgagttaccg cctcgagctg ttccgatcga
     2341 tcgcatatga cctctcaggc tccctcagat cgatggaagc aaacggagta tagaaacgtc
     2401 atcataagtc tgtcaacgcg ctgagtcgca tgaatccttt tggattttcc atgtgttctc
     2461 ggccgcgcgg ctgcacccac atttgccaca tattcgcgat ttcgcacgag ccaactgggc
     2521 accatgtgag tacttggagc ttctttgcgg cgggatttgg agcacaagtt gcgggaaatg
     2581 accaactcga tgcgatgagt cagtccgaga cgccgttttc cccgtggaat cccgcagagt
     2641 cgctgggggt gagctcgccg gaggtgaact cgcccgagcc ggacgtatac cgagaactcg
     2701 gagctgtcca gggcgacgtg gggaaggttc acgggcggtt cggtgccgga ggctcgagga
     2761 ccattcacga gcacgcagcg ttcacccgtt tttctgtgcg caactctgga gcgccttctc
     2821 acccgaccgg ctcaaacaag tctctttacg gcgtgctcgt acggccttgc caccgggtga
     2881 acggtgaccg cgttcgcgcg cggaaaacga caacgccgac ctagagtata ggtcggcgtt
     2941 gctgtgggtc ggcgacagat gaccgctcac gcgtccgtca gcgcgcgagg tctcactgtt
     3001 tcaggaactg cacggggacc tggatcgtgg ccgcgccacc ctccgggcga tcgaactgag
     3061 ccgctcgcgc acgagcctgt acgcagctca cgaccgacgg cggcaagctg cctgacggaa
     3121 ccgccgtaac gttcgacacc tcgccaccag ggcccacctg aatcgtgagc ctgatgctgc
     3181 cagaagcatc gggattttcg ttcaaaccgc gctgataaca cgcgcggaag ccagcccgca
     3241 tacccgcgac gacgcgcgct gcgttgctca cgctgccgcc gctcacggcc gctccgccaa
     3301 cgttggcgtt acccttcggg cccgcaacgg cggccgccgt tccgctcccg gaggtgcccg
     3361 tgccgcgacc agtggaacca attccagcca gcccgccgcc cacggctccc ggtcgtagtg
     3421 ctccgccgcc accacccaat cgcaggtctc cgctgccgac gccgacaccc gcgccgctcg
     3481 ccgccgcttg atcgagcgcg ccagtcgcga cttctccgcc tttcagcaca ccagccgtcg
     3541 ccggaccgct cgccgacaac gcaccgagcg tcgccatctc gagctgctcg agctggttgc
     3601 tcacggcagc ggcctcacgt tggctcagcg cgcccgcgcc aggcgcaggc cctttgggcc
     3661 cgcccccagc cggcttcggc tccgctttga cctcggcttt ttcttcggcc tttgcctcct
     3721 ctttctcctc gggcttgtct tcgaccgggg gaggcggagg caggttcttc aacgagtcaa
     3781 tcaggccggc taccgcaact tcgtcgtcta cgatcggatc cagccaatcc gagtagatcg
     3841 cgccgagcag cacgaagtga aacaaaaacg agaacgcagc aatgatcgtg ctgttccagt
     3901 cgatcgcgcc cgcaccgcgc agcactgcca ccggtagctg gggcttcggt tgcatcgggg
     3961 gcggagccac gaactgaaac aggaacgtgg tgtccccaat cacgaccttt ccgcgcgaat
     4021 cttcggtcag gcggatctga taagcccctt gcgaggtgcg ccgagcctgc cccttgagca
     4081 cgttcatgtc agagatgccg gttggcagcg caatcctgcc ttgcatccca tccaggaagt
     4141 tcaagtgata gtcgttgccg acgatctcga acaaacggaa gctcgaaggg atctggtcac
     4201 tggttatgac gaacaggttc ttttcactgg gaccgatcgt cacatggccg cgttgcttga
     4261 cgatgcgctc ttcagtcacc cgcccgttct gcaccactcc aatccgcagc accttcggac
     4321 ccgtggcagc cacggcccgc atcacggccg tcatttgacc cgggcgttgc gaaggaccgg
     4381 tacctgaatg ctgggacggc tgtgtcatgc tcgaaccttc tcgagttaag tgcgagttgc
     4441 gtgagttgcg caggctggcg gtgccctcga agttgctccg ccacgaccaa gttagcgcca
     4501 aatccgaatc atggtccact ccgaggcgct gaatctctca acggcccaag tgggagaact
     4561 ttcgccccgc ccggtctgtc ccggaggatt tcccccacat ccaccgcccc gcgcaggcga
     4621 attttgcgag ccgtagttag atccggcccc ggtttgtcaa agccatcacg cgggcccgcg
     4681 tcccagggtg gatgcggact ccgcgctgct cgagggccgc ggatcgcgcg gcccctcccc
     4741 tatgtcaggc cgcaattcta gccgcagctt gcgcccaacc ttcgcgaagt gctcgcgagc
     4801 cgaacgcgcc cgagcctgag ccgagcgccc gacgagaccg acccctagtg cgccggcgac
     4861 cccggccgcg aaggttacga gcgcggccgg ca
//
LOCUS       DASTKU010000803         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_157793, whole genome shotgun sequence.
ACCESSION   DASTKU010000803 DASTKU010000000
VERSION     DASTKU010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157793"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..2161
                     /locus_tag="VFQ61_27955"
     CDS             257..2161
                     /locus_tag="VFQ61_27955"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9958374.1"
                     /translation="MFLCGRSGRTSVWWILLAAALVPLGCTGTLEPGGTSSAGAGAAT
                     GGGNGPGSAGNTPQGMGSFVGSAGTPGFPAGGAAAGNGGGAAGTAVSTAGTGAGGGAQ
                     ASCVGAPVPDAKRVVRLSFNQLSVTLHRLLGDTFGKQIDADFEIGDRAAIARTFPPLS
                     SPREGSVITTGLWQKVDQIASAAGSYVSKHLAEVTPCGAAPSEVCARDFVKTFAESSY
                     RRPLTPAESASILQVFDEVKAIYGTIPEAIQHSVYALLQAPQFLYRSELGDSKDKAGP
                     LTPFELATNLSYFLTDGPPDAALLDAAKQGKFTTPEQVSAQVDRLLALPTTRRNLEGA
                     MFSYFALDNLATVKIDDAAFTTGTEAKPYTGVRESAYREAQLFFESTLWAGPVTGLLT
                     NKRSKINSTLARLYGVTLPSTSDESQFVAVDLPASRAGLITQVAFLASNSRPDVPSVV
                     ARGLVVNKALLCANNPPFPEDAGTQAAIADANRSLQSASERERAQFRDKTAPCFGCHR
                     SFDAYGLALDSYDAIGRYRTKDPEGRPIDPSVQLPPSLGATTAKDAVDMQAQIAASPG
                     FASCISKNMLNWALAEGSQLLPESCATQSVVTGFNASDRSYSALLKEIARSAAFMQRA
                     AGEASTGGNP"
     gene            2158..>2394
                     /locus_tag="VFQ61_27960"
     CDS             2158..>2394
                     /locus_tag="VFQ61_27960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958375.1"
                     /translation="MTNSHRFISYRFARRSFLAGLGGAFGLEILLGNMEAAAEGASSP
                     ARLLLTHFPIGTYKQSYLPKGTQTNFTLSPILAPL"
BASE COUNT          424 a          718 c          800 g          452 t
ORIGIN      
        1 gactgacccc ggagcgttac ggtgggggcc ggcgtggggt ttgggggggg gtgggttgaa
       61 aatggctgcg cgagcgagcg acacgcgagg attcgcgctc gtaattcggt ggccgcaaat
      121 ccaaaggatt ttcggcgtcc cgcggagttt tacaatctgg gctgctgccg atttttgtga
      181 acagttggta tgcctcgacg caactagagg gagactggtc ggcgcaaggt cgattcagta
      241 acccggagtc gcctggatgt ttttgtgcgg acgcagcgga agaacctcag tctggtggat
      301 tcttttggct gcagcactgg tgccactcgg ttgcacggga actttggagc caggcggcac
      361 atccagcgcg ggcgcggggg cagccacggg tggaggtaac gggccgggct cagccggtaa
      421 tacacctcag ggtatgggtt ctttcgtagg ttcagcggga acacccgggt ttcctgcggg
      481 tggcgccgca gccggcaacg ggggtggtgc ggccggcacc gccgtgagca cggcgggcac
      541 aggtgccggt ggcggagcgc aagcctcttg tgtaggcgcg ccggtgcccg acgcgaagcg
      601 cgtggtgcga ctgagtttca atcaactctc ggtgacgcta caccggctgt tgggagatac
      661 gttcggaaag caaatcgatg ccgacttcga gatcggtgat cgagccgcga tcgcgcggac
      721 gtttccgccg ctctcgagcc cgcgtgaagg cagtgtgatc acgacgggcc tgtggcagaa
      781 ggtggatcaa atcgcgtccg cggcgggctc ctatgtgtcc aagcacttgg cggaggtgac
      841 gccctgcggc gccgcgccga gcgaggtatg cgcgcgcgac ttcgtgaaga cctttgccga
      901 gtctagctat cgtcgaccct tgaccccggc agagtcggcc tcgattctgc aggtcttcga
      961 cgaggtcaag gcgatctacg gcacgattcc ggaggccatc caacacagtg tttatgcgct
     1021 gctgcaggcg cctcaatttc tgtatcgctc ggagctcggg gattcgaaag acaaagccgg
     1081 gccactcacg cctttcgagt tggccacgaa tctgtcctat ttcttgacgg acgggccgcc
     1141 agatgcggcg ctcctggacg cggccaagca aggcaagttc acgacgccgg agcaagtttc
     1201 ggctcaggtg gatcgtctgc tggcgttgcc aacgacacgg cgcaacctcg aaggggcgat
     1261 gttctcgtac ttcgcgctcg ataacctggc cacggtgaag atcgatgatg ccgcgttcac
     1321 gacgggcacc gaggccaagc cgtatacggg ggtgcgcgag tcggcatatc gcgaagccca
     1381 gttgtttttc gagagcacgc tatgggccgg accggtcacg ggcctgctga cgaacaagcg
     1441 gtccaaaatc aattccacgc tcgcgcgcct gtacggtgtc acgttgccca gcaccagcga
     1501 tgagagccag ttcgtcgcgg tggacttgcc cgcgagccgc gccgggttga tcacgcaagt
     1561 ggcgttcctg gcctccaact cgaggcccga tgtgccttcg gtggtggcgc gcggattggt
     1621 ggtgaacaag gccctgttgt gcgccaataa tccgccattc ccggaggatg ccggcacgca
     1681 agccgcgatc gccgacgcca atcgatcgct gcagtcggca agtgagcggg agcgagccca
     1741 gtttcgcgac aagacagcgc cgtgcttcgg atgccatcgc tcgttcgatg cctacggttt
     1801 ggcgctcgac agctacgacg cgattggccg ctatcgcacg aaggatcccg aagggcgtcc
     1861 catcgatccg tcagtgcagc tgccgccctc cctcggggcg accacggcca aagatgcggt
     1921 ggatatgcag gcccagatcg cagcgagccc cggcttcgcg agttgtattt ccaagaacat
     1981 gctgaactgg gccctggccg agggctccca gctgctcccc gagagctgcg cgacccagag
     2041 cgtggtgacg ggtttcaacg cgagtgatcg cagttactcg gctttgctca aagagattgc
     2101 ccgctccgcc gcgttcatgc agcgcgccgc tggagaggcc tcgaccggag gtaacccgtg
     2161 accaactccc atcgcttcat ctcctaccgt ttcgcccgcc gttcgttcct ggcagggttg
     2221 ggcggagcat ttgggctgga aattctgctg gggaacatgg aggccgcggc cgagggagcg
     2281 agctcccccg cgcggctctt gctcacgcat tttccaattg gcacttacaa gcagagttac
     2341 ctgcccaaag gcacccagac gaacttcacg ctctcgccga tcctcgcccc gctg
//
LOCUS       DASTKU010000804         4208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1274526, whole genome shotgun sequence.
ACCESSION   DASTKU010000804 DASTKU010000000
VERSION     DASTKU010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274526"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="VFQ61_27965"
     CDS             <1..1476
                     /locus_tag="VFQ61_27965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958376.1"
                     /translation="AGSSARSGGSPSGGALARGGAGAGGKSTTGTSTASSGSGGSSSG
                     EATGTGATTSSAGASTSGGQSGVGGVAATGGAPSSGGTGPDCCNSEGGAGGSSDRGPL
                     DGSSWDVHLAIELTSRPSNPVDCSSADLTAHFAARGERLSLIAGRDGLVDVGELEEAT
                     GPSPDYTLVGPLTFPTRGDCALEWMDVKELVLRALDEDADGTPDAIEGEGKATSAVVR
                     GDVVSTVQLSFVLRGHPDAQSPTLLMPLEHHPLDLVNLPASEPIALSSVVGLNLTTGA
                     RAVPLQGKPVEEVAFSAFSAPDILDFGSRWRVSPGLQDLAGLRLDSNTPQIISVLADP
                     GEFAQDGFEAAPVAHLTGSAQIVTGVGSVPALSGQRSLFVPEGSTATLHLRRAAREPN
                     LRFTARSFSIYDANPRNVPIDIGVIWGKQRFRAEYLSGNSDFTATGDSKWSSAGPSRE
                     YSVAIGETGSDVIVRVAPRVCETGMCPPPQAWLVDDVRLAD"
     gene            1545..2600
                     /locus_tag="VFQ61_27970"
     CDS             1545..2600
                     /locus_tag="VFQ61_27970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958377.1"
                     /translation="MRKPGVQWFTKAAARGSAFSVLALIAGHARAQAVAQEITAEAAF
                     VEGVQLMKANRCPEALERFKESQELDPASGTLLDIAFCEAALGRTASAWLAYRKALTL
                     AVKSEKPQHAQIAREQSEKLAPELPRLTLLVSDAGRTWKLRLDGAPLAVSGASPLFVD
                     PGKHVLEGAYHEQSFRLPFEVAAREQKVLTVPEPPPDTSHNPTPRQSSSAESSSTESP
                     RSQSAPAPANPESARNVSVGEQDKADIRTRAWLASGFGAATLLVGVGLFTHARLRFDA
                     ADCPNNQCVRDRDLRASNSARTEAYVSYGVAAGGVALLGLGAAWFVGSKPGRPESLRV
                     RAKASVAACGVALEGSF"
     gene            2600..3706
                     /locus_tag="VFQ61_27975"
     CDS             2600..3706
                     /locus_tag="VFQ61_27975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958378.1"
                     /translation="MRVLRRSKASLPPVFLLCVGKLSGCSLFEFPDRTAMQTAGESNA
                     SAGGASSGGLTTIGQTASSAGTGGKNHVASGGASLAGAGGRIAVSGGTDSAGAGVDSA
                     GAGGRELVDTGGANSAGAGGRTGENGGANAAGRGGQLVDVGGGGGGGGRLGGGGEGCN
                     GSRPFPENPVALDDFSYSGPNLSSNWDDRLTHLDPPGTYEVKDGQLAIALADLTAPMN
                     WINGEDPAGFGEFGTSQEVWATLSGFGPDLYGVQLWLKNVRVAIGYTARLNSEAALYV
                     VYEFGGHRDLMKIPVELPRSRPAVLGGRAYIDGCVEVFADGTFRGSKYLWQGDLLDEN
                     PPPTEFYSQPGMIGVTAVGPIRIDDFGGGTISPP"
     gene            complement(3756..>4208)
                     /locus_tag="VFQ61_27980"
     CDS             complement(3756..>4208)
                     /locus_tag="VFQ61_27980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HET9958379.1"
                     /translation="GSMGSGGASFSGAPGGGSAGSVALGGTSNASGGSVAAQSGAYGQ
                     SGAYGQSGAGGSAASPGGTGNGGGAPSSWGGTLSANGARSSSGAAGNPMGGTSPSMSG
                     GSFADSRDQTTNNGCSCSAVRTGSTAGVAPLSALALLLLRRRQVRRKR"
BASE COUNT          734 a         1371 c         1381 g          722 t
ORIGIN      
        1 gccgggagct cggcacgctc cggcggctcg ccttcaggag gagcactcgc tcgcggtgga
       61 gctggcgccg gcggcaaatc cactaccggg acatccacgg caagttccgg tagcgggggc
      121 agctccagcg gcgaggcaac gggcacgggc gcgacgacct cgtccgctgg cgcttccact
      181 tcaggggggc aatcaggcgt gggcggagtc gcagcaacgg gcggtgctcc ctcctccggc
      241 ggaacaggtc cggactgctg caactccgaa ggaggcgccg ggggctcctc cgatcggggc
      301 cccctcgacg gcagctcctg ggacgtccac ctcgccatcg agctgacctc cagaccgagc
      361 aatccggtcg actgcagcag cgcagatcta accgcgcact tcgcggcacg tggagagcgc
      421 ttgagtctga tcgccggtcg cgatggtctc gtggacgtcg gtgaactcga ggaagctacg
      481 gggccatcgc ccgactacac gctagtgggc ccgctcacct tccccacgcg cggggactgc
      541 gcactggagt ggatggatgt gaaggagctc gtgttgcggg ctctagacga ggatgcggac
      601 ggcacccccg acgcgattga gggcgaaggc aaagccacct ctgcggtggt gcgcggcgac
      661 gtggtctcta cggttcagct cagcttcgtg ctgcgtggtc acccggatgc tcagagcccc
      721 acgctgctga tgccgctcga gcaccatccg ctggatctcg taaacctccc ggcgagcgag
      781 cctatcgctc tctcgagtgt agtcggccta aacctcacga ccggcgcgcg ggccgtgccg
      841 ttgcagggca aacctgtaga agaggttgcg tttagtgcct tctcggcgcc ggacattttg
      901 gacttcggta gccgttggcg ggtgagtccg gggctgcagg atctcgcggg gcttcggctc
      961 gactccaata ctccccagat catctccgtc ctcgccgatc ctggcgagtt cgctcaagac
     1021 ggcttcgagg ccgcgcccgt tgctcatctc accggctcgg cccagatcgt cactggagtc
     1081 gggagcgtac cggcgttgtc agggcagcgc tcgctgttcg tccccgaggg ctccacagct
     1141 accctgcacc tgcgccgagc agcgcgggaa cccaatcttc gcttcacggc acgctcattc
     1201 tcaatctacg acgcgaatcc ccgcaacgta ccgatcgaca ttggggtgat ctggggcaaa
     1261 cagcgctttc gtgccgagta tctctccgga aacagcgact ttaccgcaac aggggactcc
     1321 aaatggagct cggcgggccc gtcgcgcgag tatagcgtcg cgatcggtga aaccggctct
     1381 gacgtaatcg tgcgcgtcgc ccctcgcgtc tgcgaaaccg gcatgtgccc gcccccgcaa
     1441 gcttggttgg tcgatgacgt acgcctcgca gattgaaggc gggctattgg caccgaagtg
     1501 gcgcgtgcaa gcgtaaccca aggtctctgt tacactcgct cgggatgaga aagccgggtg
     1561 tgcaatggtt cacgaaagct gcggcccgag gctcggcttt ttcagtgctg gcgttgatcg
     1621 cagggcacgc tcgggcacaa gcggtagcgc aggaaatcac cgccgaagcc gcgttcgtgg
     1681 agggtgttca gctgatgaag gcgaaccgct gcccggaagc gctggagcga ttcaaggaaa
     1741 gccaagaact cgatccggct agcgggactt tgctggatat cgcgttctgt gaggcagcgc
     1801 tcggccgcac cgcgagcgcg tggctggcgt accgcaaggc gctcaccttg gccgtgaaaa
     1861 gcgagaagcc tcaacacgcg cagatcgcgc gggaacagtc cgaaaaacta gcgcccgagc
     1921 tgccccggct cacgctactc gtctcggatg cgggtcgcac atggaagctc cgtttggacg
     1981 gcgcgcccct cgcggtctcg ggtgcgtcac ccctttttgt agatccgggc aagcacgttc
     2041 tcgagggcgc ctatcacgag caatcgtttc gcctcccgtt cgaggtggcg gctcgagagc
     2101 aaaaggtcct gacggtgccc gagccgcctc ccgacacttc gcacaatccc acgccaagac
     2161 agagctcttc cgcagagagc tcttccacag agagcccccg gagccagagc gcccctgcgc
     2221 ccgcgaaccc cgaatcagcc cgaaacgtgt ccgttggcga gcaggacaag gctgacattc
     2281 gcactcgcgc ctggctcgcc tctggattcg gtgcggcaac tctcttggta ggcgtcgggc
     2341 tattcaccca cgctcgtctg cgcttcgacg ccgccgactg ccccaacaat caatgtgtga
     2401 gggacagaga tctgcgcgcg agcaattctg cacggacgga agcctacgtt tcctacggcg
     2461 tcgccgccgg cggtgtggcg ttgcttgggc tgggcgccgc ctggttcgtc ggctccaagc
     2521 cagggcgacc cgaatcgctg cgtgtccggg ccaaggcaag tgttgccgct tgcggagtcg
     2581 ctctcgaggg gtcgttctga tgcgtgtctt gcgccgatcc aaggcttcac ttcccccagt
     2641 attcctgctt tgcgtgggca agctcagcgg ctgcagcttg ttcgaatttc cggatcgaac
     2701 cgcgatgcag accgcaggcg aatccaacgc gagcgccgga ggtgcaagct cgggcggact
     2761 cactacgata ggtcaaaccg cgagctcggc cggaaccggc ggaaaaaacc acgtcgccag
     2821 cggcggcgcg agcttggccg gcgccggcgg aagaatcgcc gtctccggcg gaacggactc
     2881 ggcgggagcc ggcgttgact cagcgggagc aggcggacga gaactcgtcg acacgggcgg
     2941 agcaaactcc gcaggcgctg gtggacgaac tggagaaaac ggaggcgcaa acgcggccgg
     3001 ccgcggaggg caactcgtcg acgtgggcgg tggcggaggg ggcggtggtc ggctcggtgg
     3061 gggtggcgaa ggctgcaacg gatcccggcc gttcccggaa aacccggtcg cgctcgacga
     3121 tttctcgtac tcgggcccaa acctcagcag caattgggat gatcgtctca cccatctcga
     3181 tcctcccgga acttacgagg tcaaagacgg gcaactggcg attgcactgg cagacctcac
     3241 cgcacccatg aactggatca atggcgagga tccggctggc ttcggagaat tcggcacgtc
     3301 gcaggaagtc tgggccaccc tctcaggctt tgggccggat ctttatggtg tgcagctgtg
     3361 gctgaagaac gtacgcgtcg cgattgggta cactgcgcgg ctcaactccg aggctgcgct
     3421 ctacgtggtt tacgagttcg gtggccaccg cgacttgatg aagataccgg tcgagctgcc
     3481 gagaagtcgt ccggccgtgc tcggaggacg cgcctacatt gatggctgtg tcgaggtttt
     3541 cgcagacgga acctttcgcg gcagcaagta tctctggcag ggagatctgc tagacgagaa
     3601 cccgccaccc acggagttct attcgcaacc cggcatgatc ggagttaccg cggtggggcc
     3661 aatccgaatc gacgacttcg gtggtggcac gatcagtccg ccttgagctc agcggtgctc
     3721 ggcttgggct cgcgccggtg ccgagtgcca acacctcagc gcttacgacg aacctgacgc
     3781 cgtcgcagca acagcagcgc cagcgccgaa agtggcgcga cccctgcggt ggacccggtc
     3841 cgcaccgcgc tacaggaaca tccgttgttc gtggtttgat cgcgcgagtc agcgaaggag
     3901 ccgccgctca tcgatgggct cgtgccaccc attgggttcc ccgctgcgcc agaggacgat
     3961 cgcgcgccgt tggcgctcaa ggtgccgccc caagatgatg gcgcgccgcc gccgttcccg
     4021 gtgcctccgg gtgaagcggc gctgccaccc gctccgctct gtccgtacgc gccgctctgt
     4081 ccgtacgctc cgctttgcgc agccactgag ccgccgctcg cgttggacgt acctcccagt
     4141 gccaccgaac ctgcagagcc tcccccaggc gcgccggaga acgaagctcc gccactaccc
     4201 atcgagcc
//
LOCUS       DASTKU010000805        60626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_827057, whole genome shotgun sequence.
ACCESSION   DASTKU010000805 DASTKU010000000
VERSION     DASTKU010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 60626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60626
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827057"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="VFQ61_27985"
     CDS             <1..1058
                     /locus_tag="VFQ61_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET9958380.1"
                     /translation="DLRMLLIDEAGHVLFPAARSVQPTAGSGSVVMDAPIWVRGQARG
                     SVRVIKPTVVMQKLLADMAPTVLVISLVLGAAAALAAAWIGRAIARPIEALGAFSEQV
                     SVGERKAAPPPVAGREVMRLSRAIDSMRRELEGRPFVETFAADLSHELKNPVAAIRAS
                     AEVLEDSALEEPEQARRFVQRIREASERIERLLSELLSLARIEARGVEALKATDMAQV
                     ARRVLEALGEAQSRIALHVTGDVRVRGDSVWLSRAVGNLIENALTHSADGDGVSVHIA
                     RRGERVELLVENPGEIPGNARPRVFKRFVTTRGDKGGTGLGLAIVRAVAEAHAGEAEL
                     VTAGPPKVGFRISLPGV"
     gene            1291..2118
                     /gene="uppS"
                     /locus_tag="VFQ61_27990"
     CDS             1291..2118
                     /gene="uppS"
                     /locus_tag="VFQ61_27990"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET9958381.1"
                     /translation="MLAGQRHGPTELSVVKNGNNVGNRIPQHVAIIMDGNGRWAKARG
                     MQRIEGHAEGARAVRDAVETATRIGIKYLTLYAFSVANWARPRAEVEGLMQLLLDFAR
                     RERAELRQNGIRVNVIGALDDLPTTTRHAIEDLMRYTADGDRMTLTLALSYGGRQDIV
                     EAARALAVRARAGLVLPEEIDENYFHKQMTTHELPQVDLLIRTGGETRLSDFLLFESA
                     YAELSFLPIMWPDFRPETLLEAVRDYGQKERRFGMISEQVQGHLPLQAFDPLDRIQN"
     gene            complement(2207..2860)
                     /locus_tag="VFQ61_27995"
     CDS             complement(2207..2860)
                     /locus_tag="VFQ61_27995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958382.1"
                     /translation="MHLKSRGVSMLTFGFRSAGAGSIDVECPSSMRRMSGLASVCVTS
                     RVLFVSALLLLLGCSEKPARTADDASSEESPSGTESESSSSVSTKPAKTDEDAGAEEE
                     SAAKKEQKAEPAEPAFSETMSVDEAIKAVPAGFERRNIDQETLGKPLQDAGVYEPCKP
                     GSARVKLRVAVWEGRAVGVDVTTTPKNEKLAECIKGRIRELKWDARVKSLNTIEYQL"
     gene            3040..4110
                     /locus_tag="VFQ61_28000"
     CDS             3040..4110
                     /locus_tag="VFQ61_28000"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9958383.1"
                     /translation="MLPTPERRRIDWRVICNESPSELQLSTPTLPARQLAALTAEALG
                     DPSPETADRILRHAIDFARSTLQIERCGIFLLDSENQWMTGTWGTDSKRRTVDEHSLI
                     YDFGALDREVFERARAGFPWTVYENCPLINFEDDEVGVVSRGWVACTAIVGPQGPLGI
                     LFNDTALSHTPVDEAQQWRVVMLCSILANALWRCRTFLLPSAPSRFEARPGIVRSVTR
                     LLTRDPTLSCDAMAKQLNVSSRRLARMFKHEAKSSIVDYRNELRLAAFLERVDSQGGN
                     LLQAARDAGFGSYAQFHRVFRARFGRAPSEYLLERRMPDSNVINSTLTSHEASAHFAA
                     NEGMLTAKAVGQSSVTTVEPGE"
     gene            4512..5726
                     /locus_tag="VFQ61_28005"
     CDS             4512..5726
                     /locus_tag="VFQ61_28005"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9958384.1"
                     /translation="MRLGPWIAYFLFGLALGCDASSSTPSAGSGGATSASGGSVSGGS
                     ATAGETSGAGMGGSAVTGGSGATGGMATGGMTTSAGGGSGGSSGASVSSLSGIPIPPV
                     EGNVPPPTGAASTLKIVDWAGFKGAVSYTFDDTNSSQISNFDALNGLGVRLTFYLQTN
                     KTTELANDVWKKALAAGHELGNHSHSHQQAPAEAAGAADVDQAQGILKEKFGVTAYTM
                     AAPYGSAVYSTIAKTRFLINRGVSNGLVGPNDASDPFNLYCYIPPGTATAADLNLQID
                     QARAAGKWRIVLVHGFIGGTDGAYEAVNVTEFTTSVEHSKSLKDMWIDSLVNVGSYWL
                     AQRVITAVTPTTSGSDKTWTWQMPEHFPPGKYLRVTTDGGTLKQGGVALPWDSHGYYE
                     VALDAGAVTLSP"
     gene            complement(5851..6801)
                     /locus_tag="VFQ61_28010"
     CDS             complement(5851..6801)
                     /locus_tag="VFQ61_28010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958385.1"
                     /translation="MKHPSGTTVTAGTPALGTWLLVGCVAALALACNQPPPKVVESSG
                     EQAGYAEHYPSRLGTIRSRFADEEGKAKTESGEFKSYPEALKNPDLESTRDVYELADS
                     TGKSSAYTEAALEAETVTRFMDEEKDGLHQKVAGGVNYAATQKNCSEDLGGVAASAMD
                     RGVEKQLDERLRKQSEVHRYIEDHQDKLGKANLDTLNKQADHITHASHVVHVRLELYR
                     RELEALLEDQSTVRSTLDRTIKDSDAVLADAGASKPAKTLAQKRKAAAEAAQKQLDAE
                     VEQAQRAVTEMQQRNDALKKEYEDALEAMNKSLEEHAAKK"
     gene            complement(6907..7989)
                     /locus_tag="VFQ61_28015"
     CDS             complement(6907..7989)
                     /locus_tag="VFQ61_28015"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET9958386.1"
                     /translation="MHLLINTGGGDAPGLNAVIRAVTLSALRRGFRVTGIRRGYSGLL
                     DQYPSGLVPLTRDLVRGIGDRGGTILGTVNKGNPFQYPVPTAEDPQLVRDLSDRVVQH
                     YREVGADALIAVGGDGSLRIAQRFMEKGMRVIAVPKTIDNDLGSTEQTFGFESAMAFA
                     TDALSRLQPSAEAHSRVMVVEVMGRYAGWIALYAGIAAEANGILLPEIDFDIDALCRS
                     IRHRYETGRPFATVVVAEGAKPVGGEYIVRAREAGKEITLGGVGAFVAEEIARRTGFE
                     TRSLVLGHLQRGGGPTAGDRILALRYGAAAVRLAAEGRFGHMVSYQPPSMTSVPIEEA
                     IRVTRSVPLDHDAIRTAEDLGISLGR"
     gene            8510..9694
                     /locus_tag="VFQ61_28020"
     CDS             8510..9694
                     /locus_tag="VFQ61_28020"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET9958387.1"
                     /translation="MTRIQDAPTKEAPAAWGTVLLVDDEPEFLRALVRYLSRNTLRVE
                     AYSSAHDAILRIRRGGIDVVVSDISMPGMNGLELLRAIREYEPDLPVLLVTGLPALES
                     ATEAIEYGALKYLVKPIDPEALCASVDKAMKLSRLARAKREALELMGRIGSASDRAGL
                     EADFDRALASLWVALQPIVRAKDGSVFGYEALLRTLEPSLPGPAEVLGAAERLRQLSR
                     LGRVVRQRAVKPMLDASCDFALFVNLHPADLLDPELCAPDSPLSGMAARVVLEITERA
                     PLADMENVAQRVQALRELGFRIAIDDLGAGYAGLTSFALLEPDIVKLDMALVRGIDQK
                     PVQQKLVQSMTSVCKDMGLLVVAEGIETSDERDTLIGLGCDLLQGFLFAQPGPPFPVA
                     HW"
     gene            complement(9832..10533)
                     /locus_tag="VFQ61_28025"
     CDS             complement(9832..10533)
                     /locus_tag="VFQ61_28025"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET9958388.1"
                     /translation="MQPPSSKERRRHPRGYVAAFATVFVEETLLAKETRVGEYVVRDL
                     SAGGAQLVNGPPLEPGMRCRVHLEIAGLGKLRVPAVTVRAAHTPYSNGSIGVMFTHLR
                     PDVEDGLQQLVLQELERATMPSVLVACSDIAAASAVADALAEIGERPLLALTALEALV
                     WLADPNTRVELALLGEHLMGGSPAELAATMAQEFKHIRCVTFQGPLSSTTLSRLLDAY
                     VPAPAQRPSQIALQQ"
     gene            complement(10900..11730)
                     /locus_tag="VFQ61_28030"
     CDS             complement(10900..11730)
                     /locus_tag="VFQ61_28030"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HET9958389.1"
                     /translation="MPMTIQTNISSLETQKSLGKTQSALTKNFSRLSSGYRINTAADD
                     AAGLAISESMRTQIRSYVVAERNANDALSMTQTAESVLGEMSNILGRMRELAMQGANG
                     SMTDTDRGYLETEFTALQEEMARMQQSAKFNGRLLISQTATDITFQIGLNNTASDQIT
                     LTFGGLGLTTLLSASTTVSGTTTGNSLSALGRIDGALTIISTNRAKYGATMNRLEVSV
                     SSIQTMRLNLAAANSRIRDVDVAQETAEMSRNQILSQAGASVLAQANQLPQLALQLLK
                     "
     gene            12017..13387
                     /locus_tag="VFQ61_28035"
     CDS             12017..13387
                     /locus_tag="VFQ61_28035"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9958390.1"
                     /translation="MSDWPRFDGCEVVEKLHTGPLSESYRAVQRPLGRPVLIKAIGAS
                     ILPSSPFAASLEREARLLAELDHPSIVALYDFVRRDERMWMVLEHVDGWSLEEIEKKV
                     GKLDQRGALVIASEVARALQHAHERGIFHRDVRPRNILVSKRGHVKLANFSVASDERM
                     VTAPELLDHGASYVGPSYMSPEQILGEPSDPRSDLFSLGVVLYELLAGERPFQAPDER
                     NESLRIRSEPPPPLHRRVARISPGIERVVWRCLEKMPSDRFQSAGELLEALAQLQREL
                     GIRDEPETLRRALADAGLAGVPAAPRSAPRAEPPADTKASWSRGAAALLVFSVALIAG
                     ATLIQIVSRRGEQKAGSVGGQTRLELVPEKVSYLRVVAHPWAHVVVDGQRVDTTPFAK
                     PIPLGSGTHYVRFEHPNAPVERRTVHLAPGETLLLDVSMKVVPAAVASERPLVRGTDS
                     AADNSP"
     gene            13657..15591
                     /locus_tag="VFQ61_28040"
     CDS             13657..15591
                     /locus_tag="VFQ61_28040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9958391.1"
                     /translation="MNDLTSGSNLGRYELLVRLGRGGMASVWVAREHSASLPGEERLV
                     AVKVMLPELSRHSDFRAMFLEEGQIVRSIDHPNVVKVHEVGEQDGLLFMAMDWIEGDS
                     LLAVIREARKRRPVPAELAVKIIADAAAGLHAAHELRGWDGELRNLVHCDVSPHNILV
                     GLDGRAKIVDFGVANATLHSDAANEERVKGKFSYMSPEQATAQRLDRRSDIFSLGIVL
                     FELTTGERLFRGDTPEHSLQLVRSGEIPDPRSVHPGYPDRLAQITLCALDRDPARRFQ
                     TSAEFAVALERYLVAERVLVSHASVAKLVRRVIGPNIEALRQNLREALVARDGRLHAE
                     LIPDLPAMGGTFTDVSINLPHSPALFSLSRTPQPQTLEQPAPVVPPPKPRRRVGALLF
                     GVLGVSAATVGGLWAMHRPGPSPLRGASGKLDEGGSPSLVKQQDSSVAHSESAEARAS
                     GVDVNSLPLTAPSAGVGVRPPSVGRVSAGVERAAAGERAAGDRAAAEREHLAAEKDRA
                     PAASASAKSPHYEEAVHLDEEVAPAPRASTAPKLPEPSVTPAVAPASGPTGPFNRGAA
                     LAQLNAAAARASACGQGASGSGRATVTFAPDGTASRVSVSPPFAGTASEGCVDAAFRG
                     ARVPAFTGSSVSLPWSFRVR"
     gene            complement(15663..16454)
                     /locus_tag="VFQ61_28045"
     CDS             complement(15663..16454)
                     /locus_tag="VFQ61_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET9958392.1"
                     /translation="MRHAEALGCFPTGRHYPGIAHLLKVVARGQAEEFAGDREVRSLR
                     WVSIDTETTGRDPLQDRIVEIACVIWDGTAVASKRTWLINPERPIPQEAFEVHGISDA
                     DVKDKPKFAQVADEILAEMAGCVPLAYNAEYDRKVLHAELARVPGGSTLSPPAVRRTV
                     EWIDPLVWARELHKLEKSKALTEVSQRLGIEIPQAHRAEHDAEAALRVMVAFLNDPRV
                     PRTYAAFLQEQRRLWRIFEDERRLWRNRMPEASAAKPEAAAAKAG"
     gene            complement(16451..17410)
                     /locus_tag="VFQ61_28050"
     CDS             complement(16451..17410)
                     /locus_tag="VFQ61_28050"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET9958393.1"
                     /translation="MTLPGIDTSALTTREKSEWSSYVTEFLTPCADQPVSLAQCVKEA
                     RPCKACRPAAEFLLAQARRGRVRAQVEGAYKARFSPEAVRSVDLAGAPSKGASEGKVV
                     IAEFADFECPACGAARTVLDDLVKKYPNDVKLYFKHFPLTMHPHAEKAARASVAALRQ
                     GKFWDMHATLFEHQTALEPENIERLAKEIGLDLKRFREDRDSESAADAVSRDRKQGEA
                     LQLKSTPSLFINGRPFPPTSEFSEELEEWVKSEIEIVGKSPEAAKTTEPAKAEGLKAD
                     PGKTEPSKSEPAKAPSSKGVESKPSEKSPVAPKSDPKPAAGNP"
     gene            17905..19509
                     /locus_tag="VFQ61_28055"
     CDS             17905..19509
                     /locus_tag="VFQ61_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9958394.1"
                     /translation="MSDSGPSSYHPASIPPPSTLPPPPGVRHVIAVVGGRGGVGASSL
                     SINLGVYLAQLGRKVLLIDADPTGAELHTLLGLELPPLPDTDEDPDDLEIAPLQTRVP
                     GLVLLPQGYRVGSTLPIRPGRKPRWARRLRQLNVDYVLLDLGAGTAPSTLDLFLTADQ
                     GVCVTTPEPPSVEATYRFLSAVFLRSVRRSLVEDRYRMRLVERAQSELGALPSPLHLV
                     RTIARYDSAVSARAAAELAQLRPRLVVNNVRLRTDSDLGPAMVDMARRYLGVKLDYVG
                     HIEQDDAVWLSVVRRRPLLIDSPTSKSARNTERIARRILALASARSELRSEPPLPIIA
                     EEPNLYDVLWTHRGATDEELRRAYKRQRDIYTPGSLPLTSLLGEEELKREQARIEEAH
                     ETLLDPLRRKAYDASVFREDTSSPPRAPMPRDAALEAERALLRQELSREISAETEFTG
                     RLLGKVREAQGIEIEDIAKHTRISIAHLKAIESEAFSELPALVYTRGFVQQLAKFLKL
                     DPTQVSRTYLHRMREAVSRPDEESSS"
     gene            19506..20996
                     /locus_tag="VFQ61_28060"
     CDS             19506..20996
                     /locus_tag="VFQ61_28060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958395.1"
                     /translation="MNGAPPRPRRPSDRAFGFALFWVALLPRLYVAVAWIKEPVWDGH
                     YYHFGAERIAAGLGYSEDVWVRGQLIWKPWVHYPVGYSALLGALYRTFGSSLLVAPLL
                     NALLGALLAVTTFHGARDFLGQRRAALAAALVALHPGLIAYTPLVMTEVLAAFLISAL
                     GLSLLSRRRWAVPVAGVLLGALVLVRPASLALLALLPFLRHGGVWTRIRDSVLVVLLA
                     GLCVMPWTVRNCQRLDGCALVSTNGGWNLAIGAITESGRFQTLRASDGCSAVRGQVHQ
                     DRCWAELGKRRILADPMGFLRRVPRKLAETFNHESFAFEYLHEADPIRWSEARRVAGR
                     ELTSWYHRLLLLIAGFGVVALPREFRRDRWGFAVQCALLLTFGVLSGRALMFDDKPFF
                     WLPVFAAVVGLLPLPGRPATGPGALYAAGVLLSVVLVHALFFGEDRYHMVASPMLCVL
                     AAAALRPSRGHLRSSATTWSAPENRLLNTVPGSSGVNDIYPAKLLR"
     gene            21042..23237
                     /gene="scpA"
                     /locus_tag="VFQ61_28065"
     CDS             21042..23237
                     /gene="scpA"
                     /locus_tag="VFQ61_28065"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HET9958396.1"
                     /translation="MPIELARLVSGQRLLYHGSAMRTPTENPLQYWAELAEKELRGAK
                     PESLNWNTAEGIALKPLFTRADLEQLPNVDTVPGAFPFVRGPRATMYAGRPWTIRQYA
                     GFSTASESNAFYRSALASGQRALSVAFDLPTHRGYDSDHPRVAGDVGKAGVAIDSVED
                     MKALFEGIPLGEMSVSMTMNGAVLPVLASYIVAGEEQGVAPARLTGTIQNDILKEFMV
                     RNTYIYPPGPSMRIVADVIQYTSRHMPRFNSISISGYHMHEAGATADQELAFTLADGL
                     EYVRAACGKGLAVDDFAPRLSFFFAIGMNFFMEIAKLRAARLLWASWMKRLFAPKLAE
                     SMMLRTHCQTSGVSLTEQDPHNNVVRTALEALAAVLGGTQSLHTNSFDEAIALPSEHA
                     ARIARNTQLVIEHETGVPHVIDPFGGSYFMESLTAQLSAKASSLIEEVEAMGGMTRAI
                     EAGVPKLRIEAAAAERQARIDRGVDVIVGVNRFKTDANAPVEVRVVDNAAVRETQIAR
                     LNELRRGRNEATTTAALNELAEAAARSDANLLEASITAIRARATVGEVSSALERVFGR
                     HRAEAKLVSGVYSQAYGNEQEWLDLRDQITQFAQRHGRRPRILVAKLGQDGHDRGAKL
                     IATAFADLGFDVDVGPLFQTPLEAARQAVDNDVHVIGISTQAAGHTSLVPELLQALEQ
                     LEARDIRVVCGGVIPAADRSKLLSQGVGAVFGPGTPVLEAARAVLKLIT"
     gene            23234..24217
                     /gene="meaB"
                     /locus_tag="VFQ61_28070"
     CDS             23234..24217
                     /gene="meaB"
                     /locus_tag="VFQ61_28070"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET9958397.1"
                     /translation="MTDSDSSVQELARRLRAGERRALARAITLVESARVEDDRRAALL
                     MSELLPHTGGALRIGVSGAPGVGKSTFIEAFGMHLVKLGHRVAVLAIDPSSALSGGSI
                     LGDKTRMPELSASDRVFVRPSPSGGSLGGVAQRTREAALLCEAAGYDIVLIETVGVGQ
                     SEFAVAHMTDTFLLLALAGAGDELQGIKRGIFELIDLLAITKADGENVSAAERARAEY
                     ASALRLLHQGLRGTVPEVLCVSAVEQRGIETVWSQIERAVERARASGAFDAKRAAQRR
                     AWFSECVEQELHRRFFARPGVPERFSGLQAQVFQGDLLPREAARQLLNDAI"
     gene            complement(24325..26559)
                     /locus_tag="VFQ61_28075"
     CDS             complement(24325..26559)
                     /locus_tag="VFQ61_28075"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET9958398.1"
                     /translation="MIQKIRIDSAAISEGRERRRPPSRFRPFLASFAVPMAVSLGGLF
                     GSLGCSSENKPAGTTPMPSATGTSPMPSATGTSMPPTTMPPATELTWPCEATEGANTL
                     FVRKTGCKADFDILASQPLDASIPGARSGKVVLDLMDQNQLYFQNSNKYPIHYEFAKA
                     NLSKPPNLTIGSLSDFNPQYTLPLAERRFMLGAVTYYEAPKIWALEIAPYDTSTPDMI
                     KTLFDAVKANAYYGPVLKFHPTSEAVDASARKATGVPLVTTDELYASIDYQPLNLGEV
                     VGPLKMVRSVDLDNIYVSARDIVVLDAIPNDISPTAGIITEQFQTPLSHINVLARTRH
                     TPNMGLRKATTNPLLTPLFGKHVRLTVGSFEWKVVEVTQEESNAWWEQHKPAPVTLPD
                     PDISVTDLRDVETVTEHTDVAPYVTLDALKASIRIFGAKAANYSVFATDPKVPHKKGF
                     AIPVYFYDAFMKANGFYDRVTQLEQDPNFTGQDSVRDQKLAELRADLMKADIGTELKT
                     KLKEKLDKDYPGKAIRFRSSTNAEDLDGFPCAGCYDSHTGDPAEFGGDQIAAAAEAIK
                     KTWATVWNLRTYEERKLHSIAHTKVAMALLVHTNFPDEEANGVAVTANIFDPTGNAPG
                     FYVNVQKGGQAEVVHPPPGVTSDSFLYQYSFPNQPVIYYTRSNLVNEGEHVLDARQIY
                     DLGQALNSIHERFRKAYQASPDAWYAMDCEFKFDDEAAPGQKPSLYIKQARPYADNPT
                     VSSN"
     gene            complement(26897..28294)
                     /gene="zwf"
                     /locus_tag="VFQ61_28080"
     CDS             complement(26897..28294)
                     /gene="zwf"
                     /locus_tag="VFQ61_28080"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET9958399.1"
                     /translation="MMQSELASDALVLFGATGDLAFKKIFPALYSMLRRGHAPFPIVG
                     VSRGGSSLEDLRSRVRASVSNNNGLDEKAFSALADNLHYVDGDYRTVELFDKLRQTLG
                     DARRPLYYLSIPPSAFPNVVEFLGRSGCSEGARVVVEKPFGRDLASARHLNQVLHSVF
                     GESSIFRIDHFLGKEPVQNLLYFRFANAFLEPLWNRNFVSSVQITMAEDFGVVGRGRF
                     YEEAGAIRDVVQNHLLEVVAFLAMEPPPAGDREALRDEKTKVFKAIRPLSPENVVRGQ
                     YKGYREEEGVAKDSTVETFAALRLHVDSWRWAGVPFYIRTGKNMPVTATEVLVDFHRP
                     PHMVFPEPVPANTNYVRFRLGPDIAIAIGARAKRPGDAMAGESVELMVRNQRADEMGA
                     YERLIGDAMRGDPNLFAREDSVEAAWRIVEPVLGDVTPIYEYDKGTFGPQEANYVLHG
                     SARWYDSGKPTRSLL"
     gene            complement(28291..29328)
                     /gene="gnd"
                     /locus_tag="VFQ61_28085"
     CDS             complement(28291..29328)
                     /gene="gnd"
                     /locus_tag="VFQ61_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET9958400.1"
                     /translation="MQIGMIGLGRMGANMVRRLMRAGHTPVVYDVSKEAIATLVREGA
                     EGATTLSEFAKKLQKPRAVWLMVPAAHVDSTVRALSAELESGDVVIDGGNSHYHDDLR
                     RAHDLAARGIGYLDVGTSGGVHGLERGYCLMIGGDSAAVNRLDPIFAALAPGATDDAP
                     TTAIPQGDASHTNAMATAARGYLHCGPLGAGHFVKMIHNGIEYGLMAAYAEGLNILRH
                     ANAGHQTREKDAETTPLSHPEHYQYDFDLAAITELWKHGSVISSWLLDLTADALAKSP
                     RLEEFGGNVSDSGEGRWTVQAAIDIGVPAHVLSASLFDRFASRMQGDFANRVLSAMRL
                     GFGGHHEKKST"
     gene            29710..29886
                     /locus_tag="VFQ61_28090"
     CDS             29710..29886
                     /locus_tag="VFQ61_28090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958401.1"
                     /translation="MATCKGCGEEVDSLVSVKVGGKTKKLCEDCADRAREEGEIAEAS
                     ESVVQNMMGFKGRR"
     gene            29894..30583
                     /locus_tag="VFQ61_28095"
     CDS             29894..30583
                     /locus_tag="VFQ61_28095"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9958402.1"
                     /translation="MKPLISGWVLLCFVVLSSCREPAASQTRAGRRPASVGTSSSSSL
                     PVTATEAAQAAATPGAVQNASDRGRTQLSAIRADQLLATIRGFGAKATLVNAWASWCG
                     PCRDELPMLESLAQNLSRDDVRLVLVSVDEAEDGAKAEAFLRSVGNRSPAYLAAPPLS
                     EFKVGMNPRWPGMIPATFLFDAAGKLRYFWGGPVYEHEITQVIEGLLAGKLIDGQSDF
                     TLAPGKTEAGH"
     gene            30917..31912
                     /locus_tag="VFQ61_28100"
     CDS             30917..31912
                     /locus_tag="VFQ61_28100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958403.1"
                     /translation="MPMTDIDYPGRSGAGGLYRFCRFMLLPLGLVGAVIVSYRNDWLR
                     EGARKFGYEKHYLSLEQSLVGTPGWGTPRELSQSLEAEQAALSGSVEANSNRRSAGSS
                     SSAGDQPDREDSEGTAAERAVAEGTDAAGQRHADSEQAAELEQPAKDPGARRSAAESV
                     SAQSEATTRQYAVAAQEPEPSSPVQRAALAQSAAVLSRASGNQRSSDAQRSSELQRSH
                     VTRAEEATVAPRVAAKTRVTREVIEDEDDDKPAASSGPSKKALELIAKRAAALAKSGE
                     SSDKVALAEKPKPVEKPKPEKAAKPEKAKPAAPPPPPLADTPLNAAIRASIVNGK"
     gene            complement(31921..32451)
                     /locus_tag="VFQ61_28105"
     CDS             complement(31921..32451)
                     /locus_tag="VFQ61_28105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958404.1"
                     /translation="MKYLPLALLVVACYSPGPYGYSRVYSPLDAEEHAAETAREYDPV
                     MAQRAPADWKKTPVSVFGIVKNRQDGPGGLAQLTLGIRTLADRNLCDDADEESCRVTI
                     GEREHAVIHAQVKLLGEDDLGRRRVQPGSLVRVIGRLRDDRDPADGEPVLSGDYYRHF
                     PRGEYVTRGDSEHMRR"
     gene            complement(32557..34530)
                     /locus_tag="VFQ61_28110"
     CDS             complement(32557..34530)
                     /locus_tag="VFQ61_28110"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9958405.1"
                     /translation="MSFVVVAALAITAFVAAPFLAHLLRRGRARELVFPPAAWVPAAR
                     SVARQRARLEDRFLFLLRALLIATLALLGATPLLRCSRLALDRSGGASVALALVLDDS
                     QSMRARLPDGGTRFERARNAALELVGTARSGDAVTVVLAGKPARIALAATRDLAAARR
                     TLQELRVTDRGTELGGAVKLAQSMLASLPQRDRKLALLSDFAGEIGDEARELWVPLPE
                     LAARTENCGIASAESDGHGVSVQVACTSMRAAQGRTVSLVAGEPSGAAASAAPAAATE
                     ALQARPGLQTLRFGAAPAQARRVKLSGEDAISEDNEGAIRRGSSALGIGVWTQAQEAH
                     ADAFGPGLVESALEALGSDLRVRPLAVLPEEPKELASFAGLVLDDPNGLGPEVRDGIE
                     TWLKRGGVALALLGPSAESRSIGATLEPFAQGGLPWEKTRAKGVDAASIAWLGPEASA
                     LAELAPQGRVHLAGEASVESRVAARFDDGAPFLLEREFGLGLVWTLALPSSPELSDFA
                     LRPGFLALLDQFVEAAHERASSASSVPGVPFRFAAGSQAEVSDPSGKRLSSEALSTDP
                     RRQLFTPELAGVYRVRTHEGTEERVVSLDASELLTEPALPPTQALSRSAGVGVREVDV
                     SGQVVWVLLAWTALELALRLLREMAQRRASPTA"
     gene            complement(34527..35447)
                     /locus_tag="VFQ61_28115"
     CDS             complement(34527..35447)
                     /locus_tag="VFQ61_28115"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9958406.1"
                     /translation="MAIREKIDWGKLAPLRIRAQAVADGVYAGAHRSVRRGAGIEFGG
                     HRNYVAGDDLRFLDRHARMRHGLLLIREFETETDRSVRLLVDASASMAYRSEWASLSK
                     YEYAALIAAALGRIALLGGDRVALDFIGSHEQTRGLPSTGGREAFDRVVGHLEAITPA
                     GPVTSTDLERSVAPSLRYARRGSIVLVASDLVDLPETAAELLSGLGAAGRALIVLRVL
                     DPAELTFPFHGPIALRAAEGSTVVETDADPVRAAYLEALERATRAFEQRVIARGGRFI
                     ACSTQDDPVAIVRQVLLGIERAGESLSQNS"
     gene            complement(35463..36452)
                     /locus_tag="VFQ61_28120"
     CDS             complement(35463..36452)
                     /locus_tag="VFQ61_28120"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9958407.1"
                     /translation="MRVFPTVSGRTLRARLQSTPFWNSGRAVIACVLWTLVSLLGQPT
                     PALAFTHTVRTGDTLASIAEKYYGKIQFEHVLVAANWLDVEGGSSIVRGMRLEVPALS
                     HRVIARGETWDALALELLGDERRSDVISMSNDSKPWLLPEEGAEIVVPYNLRLLVKPG
                     DTLVSIAMKFMGDMNRAWVLAHYNRLEKNGLEPGSVILVPLTNLPLTEAGMKAAARDA
                     GNLCSESAGASLKTQRRIAQEIPLLISDVRAGRYVEAIARGSRFIATGALTEPQLSVV
                     HRQLLEAYVAVEATGLAHAACEEWKKRDPQAKLDPLEVSPKILRVCEDPKPNP"
     gene            complement(36563..37786)
                     /locus_tag="VFQ61_28125"
     CDS             complement(36563..37786)
                     /locus_tag="VFQ61_28125"
                     /inference="COORDINATES: protein motif:HMM:NF016643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958408.1"
                     /translation="MPPPAPATTAEVVLTFLESVGRRSEAELYLRLFRKLPKESFAII
                     SPGGPVIRHALGSLVEQLRFLGDLGLFAPIVLGLFGTEGAQASADRLLRRLPTVGLPA
                     IPHDMNEPHLAERVREELNRERIPILHFRPAPELRLPDRVRELAELSLALDTRKIVLL
                     RRRGGLMSKVGDRPVELGPGHALPTSSGRISVINLRADREALLESRVLGKREAELLEA
                     ASAFIAANDPTPPLVSITSPLNLLKELFTVKGAGTLIKSGTVVQRLGSYSELDRERLT
                     RLLEASFGRSLLSEFFNRPPLAIYLEPSYRGVAILHDEGPAPYLSKFAVEPEAQGEGI
                     GNDLWQAICRDYRSLFWRSRNDNPITPWYQSVCDGMLRLPRWSVFWRGIESERIGEIV
                     TRADQLPADFQDAKT"
     gene            complement(37788..38723)
                     /locus_tag="VFQ61_28130"
     CDS             complement(37788..38723)
                     /locus_tag="VFQ61_28130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958409.1"
                     /translation="MLNGPILRGDRCVLEFGDLLFEVLPGVAGRITRVSLGGLELLTG
                     PEIDPGNYGSTFWTSPQSDWPIWPPPAGIDRAAFAVRELVNGFELSGPPLDDAALPKL
                     NGLSLTKRFRADLDRRAIHAEYELTNHANVARSTAAWEITRVPAGGLTFYAADSLPFT
                     PPERAAISFSQGAGCLWYTHSIRDVKAKANGDGKGWVAHVTREGLLLVKVFPDLSPAQ
                     AAPGEGEVEIYADPTYVEVENQGPFSTIPPGGRTTLRVHWYVRRLPSNVTIRAGNPQL
                     VQLASELVALDASSPAREVRPSSPNRFGYGSRAGF"
     gene            complement(38912..40318)
                     /locus_tag="VFQ61_28135"
     CDS             complement(38912..40318)
                     /locus_tag="VFQ61_28135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958410.1"
                     /translation="MHRHALCLAPPHSCLSKLLLAGALLTTLGALGCSSETDPPGGMG
                     GSGGTGGAGGTGGSGGTGGAGALPILERPAQLAYDCSVSSPARLLDVPTNDTFTGGLA
                     TLGSNAFVAWAQAPKWDAPEQKQSVRFAPISGDAQLGAVSTAVEQTSEYFSNVLLVPG
                     ADGMTLLAMRQGERELLVGQVSADGTLRAPMKPVPRDGGAASRMLAVRADTGIALLWD
                     ELADGEGRRYDLKFGLLTDDGTLTVSPNAVATNSNWLWPAAITSTGQGFLVSYVENGW
                     DGTNAFQVVSTRRIAADGTLGDEHELARSTHSDGGGSHALLWRGGEALLARSFTEGTY
                     EGEKLGSTVLLTRLDADGRRLADDVRLQSWEKSRVNNQPFLFARGEDVGLLWSQGSYI
                     YVCAGCMPDNHLEFVLLDGSTWAPKSNRLQFSNPAASGGLISPEGVTLGDDILVASRV
                     GYHVTSELSAGTIRCLPR"
     gene            40616..41377
                     /locus_tag="VFQ61_28140"
     CDS             40616..41377
                     /locus_tag="VFQ61_28140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958411.1"
                     /translation="MTRAGFLAPSALLANPARTWRPFARKSSVLLGVFLLASTVTAQR
                     GESPEALLRRYPFDPACPFGRISNGKGMIVRCIEEREATGLLQQSAGQPTPALSAHAG
                     SSQQSPGQGPAPGARDEGTPALSSTTPSTTNPPAETEPVEAPGSDRFDVVVGPVAADQ
                     GDLPLGKLGQAKSRYAKCLADNGGLRDKSAEVQVRFLVRSKGIAEGVSVQKRIGVSAE
                     AARCVAEVVDRRRVGTPSAPMVGASVTIRFEKTGK"
     gene            41374..43242
                     /locus_tag="VFQ61_28145"
     CDS             41374..43242
                     /locus_tag="VFQ61_28145"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HET9958412.1"
                     /translation="MTDAASGAHADRYRAGVTRAQAEAERLGARSRLLSNLRGLSFGT
                     ALLSGIAGFAAEASRGLSFTICGLAVLAFATFVVLHGRILEVLDLAERRVDVYQSALL
                     RLAHAWDKLPDVAEGLAPNEHPYSGDLDLFGSGSLFQRLSVARTRFGRERLAGLLLEA
                     DPNQLDEIQARQAAVQELAQAHELREEFEAHALGVAGKTGAASGAPRVRLAPNPTQLL
                     SWAESEPELLNDPASVWGVRILPPLTLAALVAWLGFGLTFVPALGLLFLQGVILMRAQ
                     RAASRVFNAVSVTEGAFLRYSELLRILERAKFEAPALRALVQRLDTPVGLPSSAMTRF
                     RRWAGWFDLRHNGLVYPVVDLVLLWDVWCTLGLERWQRAAGKHLRGWFEVIGWFEAYS
                     SLASFASDEPGVSYPELARTGTEFRAEQLAHPLIDPARRVANDVLLGTEQRALLVTGS
                     NMSGKSTLLRAMGLNAVLAFAGAPVTARRLRLSPIAVRTSIRVADSLERGVSHFYAEI
                     VRLKGVVDSASNPTPVLFLLDEILHGTNSRERQVGARWVLTELLAHGAIGAISTHDEE
                     LCRLPPHLMQSVALVHLRESVQGGAMHFDYKVYPGPVTSGNALRLMRVVGLQVPLE"
     gene            complement(43323..43853)
                     /locus_tag="VFQ61_28150"
     CDS             complement(43323..43853)
                     /locus_tag="VFQ61_28150"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HET9958413.1"
                     /translation="MTTPQTTVDLVAKPRLSSGAPPRPSLAHVAFSFLGGLLAIAAVG
                     SLTDVTGTPLVLGSFGATCVLVFGYPESPFSQPRHVIVGHVLSTTTGVVVAALLGYHW
                     WSMALALALAIALMQLTRTVHPPAGSNPLIVMLGHQSWAFIVKPTLLGAVIVTLVALL
                     FNNAVPARRGYPKYWY"
     gene            44304..45173
                     /locus_tag="VFQ61_28155"
     CDS             44304..45173
                     /locus_tag="VFQ61_28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9958414.1"
                     /translation="MTQVEPGPPPDQGTGSDCLVVIYTSESGLLGKRFSLDRSILRVG
                     RGTDNHIVLEGDSVSRRHAHLERRGSDWYVVDDGSTNGTYLNEEQVVRESVLGNGDRI
                     KIGPTILKFLSGLDAEAKYYEEIYRMTIVDGLTQIHNKRYLFEALEKELIRARRYERE
                     LSLVMFDIDYFKRINDQFGHLAGDYVLRELARVVQERIRRDEVFARYGGEEFVIVLPE
                     TGLGGALALAENLRKRVNSHGFVFQGDSIPVTISVGCAQLSKDDRTAPDLIQRADEKL
                     YEAKRGGRDRVEG"
     gene            45186..46541
                     /locus_tag="VFQ61_28160"
     CDS             45186..46541
                     /locus_tag="VFQ61_28160"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET9958415.1"
                     /translation="MARSPLLAEQPPLLRGPLPGPRSLELLKSLSAVAAPMGPRPRLE
                     AEPAQPDPRDGATESRIVYASARGVNVQDADGNIYVDLAAGFGSLLLGHSHPAVLAAL
                     SEQSQRLMQGLGDVYPSELKLALLQRLTRLYPGSARACLAQSGSDAVTCALKSAALAT
                     GRPRILAFQGAYHGLGYGPLAACGLRESYRTPFTEQLNPHVTWLPYPANEREAEASLD
                     VAQRELARSPVGAVLIEPVLGRGGVIVPPATFLPDLGKLAREGGALLIADEVWTGLGR
                     SGHWLFSLDHGVIPDLICLGKGLGGGLPMSAVLGRSEIMQHWSREAEVVHTSTFAGAP
                     LACATAIATLQVLERDELVTRARCVGEGFLSRLRALSTPAVRLSCRGAGLMIGIESER
                     PTEGWGARLQKRLLARGYITSTGGGARDVLVLTPPLGISQAPLDRFVTALEAALSEVT
                     T"
     gene            46538..47680
                     /locus_tag="VFQ61_28165"
     CDS             46538..47680
                     /locus_tag="VFQ61_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-protein synthetase"
                     /protein_id="HET9958416.1"
                     /translation="MSQSALIAESEALHERVRAFAKQARGEADFERLALEIAEYQARY
                     QPGYARLLRRCGKTPDGVAALPAVPTDAFRLTRVAAHPPALDSACFVTSGTTGQDQGR
                     HYFRTTRTYAELSVEFGRRALRSSPARCTAVALAPAPSEPPSSSLGFMCGLFMEQFDG
                     RPLVEAGHGVTAAFDVTAAERWLLSPGHIDLEGLRRAVACAQRRAEPMLLLATSFALV
                     ETLDALDRAKVTTLPLPAGSVVMQTGGYKGRTREIPRAELRTWVARVFGIAEEQVASE
                     YGMTELSSQLYEACVPGAELAGPRELHLAPPWLRVSAVDPVSLEPVAEGEVGLARFVD
                     LANVDSALVILTRDAIRKRGPFIELLGRAPGAPPRGCSLAIEALLA"
     gene            47689..48831
                     /locus_tag="VFQ61_28170"
     CDS             47689..48831
                     /locus_tag="VFQ61_28170"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HET9958417.1"
                     /translation="MNASPDKQARVRIDRLLAAARALQDPGSFPGRDLRARLLGSTGL
                     SEAGVELGLTEYLETHPSETELDALCRAVQPSPVSHVLLSANVFIAAHRALALALASS
                     ATVYVRPSRREPEFLTALAALDPELFHVVDTLEPKSHEQLTAYGRAATLAELRQQLPA
                     GVQFSAAGPGHGVAFIHESAWRASPGTPGRQTSDHAPDGTPIDQLSRELVRDVVAFDQ
                     RGCLSLRWVVVECSEPSMARLERLAVGISTALSAAEARVPLGRLTGEELGERRRYLDT
                     MSYVGEVIDNGRSSVSVLSPEAAPLLPPAGRNVLLVCTADAASYAKRYADSITSYCVS
                     HAEQPDFERAVQKCLPFARKATAGRMQQPPFDGPVDLRPATRPSTL"
     gene            complement(48846..49688)
                     /locus_tag="VFQ61_28175"
     CDS             complement(48846..49688)
                     /locus_tag="VFQ61_28175"
                     /inference="COORDINATES: protein motif:HMM:NF021516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
                     /protein_id="HET9958418.1"
                     /translation="MRPRLPPSGTQPRIGERAAAQNWRRCSGPARIALALGAGAALCL
                     DAAICRACPNCELGRAARASVWTEHFGANLTVALLPFLAVAVIAARSESGASSGRANS
                     GRGVGGEHDAGSSARRAHYASPLVSAGLLLGTGLGGFVDGILLHQILQWHNMLSSIRP
                     PTDLLSMKYNMVWDGLFHTFTWVMCALGIWRLWAAARRSEVPWSASTFVGAMCLGWGL
                     FNFIEGLIDHQLLGIHHVHPGSGELAWDLGFLALGLLQIVGGWAVLRHASSTAGGDRQ
                     LTHT"
     gene            complement(49760..51628)
                     /locus_tag="VFQ61_28180"
     CDS             complement(49760..51628)
                     /locus_tag="VFQ61_28180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9958419.1"
                     /translation="MLRQIGVEAEKLLSRAADLAFPWLAARAEQKAPPPLHPKWAPAP
                     LLRARERTSPPLGFPRQTDSLCPVCVREARSAVLAGHQDYRAFVDSNPGEIKANIEER
                     DGRIWMTKTCPKHGRFEDVMSIDPAFLRRIESLFPGRDFESPKSELRQHGTSSIKYGR
                     GSVLTVDLTNRCNMMCDPCFMDANQVGYVHELDFEGVKKILDDALSIQPRRQLSVQFS
                     GGEPTLSPHFLESVRYAREVGYFCVQCASNGIRFAQEPEFCMQAKAAGLRLCYLQFDG
                     VTNEANSHRKVGNLFDVKLKAIENLARAGIDVVLVVTVVNGVNDDQVGEIVKFAIDNA
                     DKITVVSFQPVSFTGRDEDISDEDRARQRYTLSHLAHDLKSQLGQTEPLRDWFPLSAM
                     NPMSDVVDLLLGQSAEFGALKCGCHPNCGIGTVLLVHKRTKRMVPVAEFLDIENLLND
                     MKRIADAGLGRKQTLLGMSLALVRNFHAERAPEGYGLLELVRQFQSQIGAGGRSIGES
                     EGDAKQFEWRFLFVAGMWFQDLFNYDFRRTEMCIIPYGTELGEISFCAYNTGVGFRQI
                     IEKMKMTATVAEWYKQHGKHPVYAKQQHLPLAELAAEGTPSHVIARRRLPLVASSP"
     gene            51889..53031
                     /locus_tag="VFQ61_28185"
     CDS             51889..53031
                     /locus_tag="VFQ61_28185"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9958420.1"
                     /translation="MSPFVILSDLHLDPGTHATAEACARLIAAHPGYEVVLSGDIFGL
                     SEDPPSLTPQTSVQKTLAAHPELARAMRQHLLGGARLTWIAGNHDAGLTVPGMAETVL
                     RSLDLPEAPLRIEPWFIRRGGVHIEHGHVWDADNAPVHPLGDWSLLTEPLGVALTRRF
                     VSKHASHFAHAHETTLVQGLKRAFGLYGARAPLLILIYFGVAAGICLEALLQTEMDEE
                     RQQGAGRSRALAEECGISLESLQELLSALPTPTHTEFGSTFFRLYFDRVLLALLCGGA
                     GVWSLARLSPIGLGVGVVSAAVLRHNVKRTGARYQNLPIRRLRHGAAIARSLMRADVV
                     VFGHTHVPTCEDGYVNTGSFAYFDKQLGRPYVHVDGQAHLELRRFV"
     gene            complement(53063..56095)
                     /locus_tag="VFQ61_28190"
     CDS             complement(53063..56095)
                     /locus_tag="VFQ61_28190"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958421.1"
                     /translation="MSEPFAHRRASWALSILLSAAGPGCGVGSGGEIAPTASGSSGSG
                     GNPGTSRNSGGTTATAHPALPNICTRAQARCTERGTLEVCDGGRFVPQVECGDFGRCN
                     AAHKSCLACAPGTFRCTGAVLEQCELEGLSYQVVRRCGEKETCHAEGELGYCEICSRD
                     AMYCEAFPGSAPSLRSCNALGSGTQEAERCDSSAPLCDARAGRCRTCVPGRATCELGK
                     LLKCDEAGAQLNLVEDCGHRELCDAELGVCKSNLCNSPKAAPGSVVCRGTTETELSIC
                     RANGQWELLDVCSSPVACELGLAVKACPPPELCAAGIARCSGSELQHCVLANSRMADE
                     NGYYAGDAAGYVTIAHCTRACVEDGATPAHCESGASNSSPYVESLVCVPGATEYERCD
                     SEQPQCQRETCTTGELCANGTLGCRRCVPGALRCDGTRLVQCSRTGEREQLLQDCGPE
                     ACDAYLGRCLPAAAGERYCEGPELKLMARYGTPRVLRDCGDAAACDPIQGCLAASCEP
                     GTSTCSSDKKRAYVCETGQGFSTHPHLCLPGSHCEEGFGCVRPVRVTAGAAHTCVLFE
                     GELGRDPEQDPIDVPRILECWGANESGQLANGSSLNGDEPQPRAVVFASEPSSSGSTP
                     GLYPNAAGANAPNSGAPNSAAPGSGPANSSLLRSNIRFSGGGLCAGRDFTCADVWFGP
                     SPGFYDVVCWGANQSGQLGNGVKPDAKATEVENRVQFPASRYAIFAGGSPTGSAASSS
                     APGSSTSTPPARANFPELFGVTCGSDFACALDAKGQAYCWGGNAFGQLGTGSAGSAGF
                     LPTLVSSPESFVSLAAGGRHICGTTANDLLLCWGANDFGQAGQDTRIVARASPVGEQA
                     VSQLALGRDFTLAVTARGSQPLVFGNNWFGQLAVLGSTVKLPVMAELLPSDSPDWLVS
                     GPNAAHVCGSFDSSLYCWGANHAGQAGSALLAQLNEPTELEVALDLEAPRQLALGEAH
                     SCALLRNNDVLCWGSNQRGQLGLTPSALRETKPTRVEF"
     gene            complement(56227..56817)
                     /gene="orn"
                     /locus_tag="VFQ61_28195"
     CDS             complement(56227..56817)
                     /gene="orn"
                     /locus_tag="VFQ61_28195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HET9958422.1"
                     /translation="MPSNQRIQRTQSADNLAWIDLEMTGLDAQTDVILQAALVITNAA
                     LEPLEEFSCDIFQPAAELAKMGPYVRDMHERTGLLERVQRSRTDLRSAEKQLLERIAG
                     WCPYPALLCGNSIGQDRRFIDQYMPGLAAYLHYRLVDVSTIKVLSRLWYGEAGVYAKP
                     TQGEHDAVFDIRESIAELQFYRRTLFRSQNGVQTQE"
     gene            complement(56831..57346)
                     /gene="yhbY"
                     /locus_tag="VFQ61_28200"
     CDS             complement(56831..57346)
                     /gene="yhbY"
                     /locus_tag="VFQ61_28200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET9958423.1"
                     /translation="MSESSRTRRAPRTAKAARPRPTPTQNAQRKPGQRKPAQTKSAQT
                     RSPSKSAPTSKKPAAAPRTAAPLELTGAQRRFLRAMAHDFKPIVQIGRGGLTDAVASA
                     IDTALETHELIKVKLSADASLEGDEIAAGVEQRTGSSVAQVIGHTLLLYRRRAEKPSI
                     VLPKRKERRDN"
     gene            57548..58672
                     /locus_tag="VFQ61_28205"
     CDS             57548..58672
                     /locus_tag="VFQ61_28205"
                     /inference="COORDINATES: protein motif:HMM:NF024918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HET9958424.1"
                     /translation="MRILYGVVGEGMGHATRSRVVLEHLAQAGHDIKVVVSGRAHGFL
                     VDRLRNHPNVSIEQIEGLTLSYLGNELDHGASIFNNLRNAPKAVARNVEVYRNVAEAG
                     FRPELVVSDFESWAALYGIRHGLPVISIDNMQIINRCRHERALLKGAGFDFRLTRLAV
                     KAKVPGAFHYLITTFFTPAVRKKYTSLVPAILRPEIQSARREPGEHILVYQTQSTHHD
                     LIQTLKRLPYQFLVYGLRRDAVDGNVRLCAFSETQFVEDLRTARGVLAGGGFSLMSEA
                     VSLGVPMLSVPVVGQFEQELNARYLAELGFGEYTPELSREALQRFLSRLDEYSMRLRE
                     RPHYDNSMLFGCLDELIDRVQRGERRVVALDHAVLGKYDQ"
     gene            58677..59402
                     /locus_tag="VFQ61_28210"
     CDS             58677..59402
                     /locus_tag="VFQ61_28210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HET9958425.1"
                     /translation="MLSPRVRLFVVLATVFVTCLTVGDIIGGKLIETQLLGQSFIVTV
                     GMIPFPVTFLLTDVLNEFYGKRAARFVTVVGFMMAVLAYAFIYIAVVIPIAPLTQAPD
                     FTGVRSAAFDNVFAGSRRMILASMVAYITAQLVDIFVFHALKRVTANKLLWLRATGST
                     VVSQFIDTVVINSVAWVGILEADQIMRVVFSAYSLKILIAIGLTPLIYLAHSVVQRGF
                     GIEPVLLDAQGEPMLAPEAAKAE"
     gene            complement(59434..59955)
                     /locus_tag="VFQ61_28215"
     CDS             complement(59434..59955)
                     /locus_tag="VFQ61_28215"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HET9958426.1"
                     /translation="MLHRPSVAPDKPQIESAPQQGAYQAPSVASTAAHDNPHFAAVGG
                     STAVAELVDRFYQHMDTLPEAAQIRSLHPPDLTPVKEVLRRYLTEWLGGPALYSVERG
                     HPRLRRRHLRFPIGAAERDAWMRCMSLALNEVVSDPKLRQELTEAFFKTADFIRNDPD
                     HTHHHHSTQEPSQ"
     gene            complement(60193..>60626)
                     /locus_tag="VFQ61_28220"
     CDS             complement(60193..>60626)
                     /locus_tag="VFQ61_28220"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HET9958427.1"
                     /translation="GFDTHANHDRDQVRQIYKLWGGLAYILDQLDTFGLGQNTFVVVS
                     SDFARGPRYNGENTNSGKDHWPVTSTLVLGPGIEGNRVIGGSTADQLALKLNPGNLST
                     GSDGVKLTPTVIHRALRGLAKLDPDLEKRFPLAGEALPLFA"
BASE COUNT        10293 a        19218 c        19778 g        11337 t
ORIGIN      
        1 ctgatttgcg gatgctgctg atcgacgagg ccggccacgt gttgtttccg gcggcgcgca
       61 gcgtgcagcc gaccgccggg agcggcagcg tcgtgatgga cgcgccgatt tgggtgcgcg
      121 ggcaggcgcg cggcagcgtg cgagtgatca agcccaccgt ggtgatgcag aagttactcg
      181 cggacatggc tcccaccgtt ttggtgataa gcctggtgct cggcgcggcg gcggcgctcg
      241 cggccgcttg gattgggcgc gccatcgcgc gtccgatcga agcgctgggc gcgttcagcg
      301 agcaggtcag cgtgggcgag cgcaaggccg ctcctccgcc ggtcgcgggt cgcgaggtga
      361 tgcgcttgtc ccgcgccatc gactccatgc ggcgcgaact cgaagggcgt cccttcgtcg
      421 agacctttgc cgcagacctt tcgcacgagc tcaagaaccc ggtagccgcg atcagagcca
      481 gcgccgaggt gctggaagac agcgcgctcg aggagcccga gcaggcgcgc cggttcgttc
      541 agcgcattcg cgaagcttcg gagcgaatcg agcgcctgct ctccgagctg ctcagcttgg
      601 cgcgcatcga agcgcgcggc gtagaagcct tgaaggccac cgacatggcc caagtcgcgc
      661 ggcgagttct cgaggcactg ggggaggcgc agtcgcgaat tgcgctccat gtcaccggtg
      721 acgttcgcgt gcggggtgat tccgtttggt tgtcacgagc cgtcggcaac ctgatcgaaa
      781 acgcgctcac ccactcggct gatggtgatg gcgtatcggt gcatatcgcc cggcgcggtg
      841 agcgggtcga gctgctcgtg gagaacccag gtgaaatccc cggcaacgcg cgaccacgtg
      901 tattcaagcg cttcgtcacg acgcgtgggg acaagggtgg cactgggctc ggcttggcca
      961 tcgtgcgtgc ggtggccgaa gcccacgccg gagaagccga attggtcacc gcagggccgc
     1021 ccaaagtggg tttccgcatc tcactccccg gggtttagct gcgcgatcag cgctggactg
     1081 cgcaaactca ctcccattgg cagcgtcagt ctgctggggg gtggtggccc cgctgccgca
     1141 acggaaccct gcaaccaacc acgagcccgt cagcctctcc tcaaaagatc ctcaaacgcg
     1201 gacgcttagt tcgtgggatg taacgtgcac cccggcaacg cccgagggtc ttgttcatct
     1261 actgaagtta cccccactga agtaagagcc gtgctggctg ggcagcggca cggaccaacg
     1321 gagctgagtg tggtcaagaa cggcaacaac gtcggcaatc gaatccccca acatgttgcg
     1381 atcatcatgg atggcaacgg ccgctgggcg aaagctcgcg ggatgcaacg catcgaaggc
     1441 catgcggagg gagcgcgcgc cgttcgggac gccgtggaga ccgcgactcg cattggcatc
     1501 aagtacctga ctctgtacgc attcagcgtc gcgaactggg cgcgccctcg cgccgaagtc
     1561 gagggtctga tgcagctgct gctcgacttc gcacgccgcg aacgcgccga gctccggcag
     1621 aacgggattc gcgtcaacgt aatcggtgcg ctcgatgatt tgccgaccac gacgcggcac
     1681 gcgatcgagg acctgatgcg ctacacggcg gacggcgatc ggatgacgtt gacgctcgcg
     1741 ctctcgtacg gaggtcgaca ggacatcgtg gaagcggcgc gggctctggc cgtgcgggcc
     1801 cgggcggggc tcgtgctgcc cgaagaaatc gacgagaact acttccacaa gcagatgacc
     1861 acgcatgaac tcccgcaggt ggacttgctg atccgcaccg gtggcgagac gcgcttgagt
     1921 gacttcctgc tgttcgagtc ggcctacgcc gagctctcgt tcttgccgat tatgtggcca
     1981 gatttcaggc cggaaacact gctggaagcg gtgcgggact acggccagaa agagcgccgc
     2041 ttcggcatga tctccgagca ggtgcagggg cacttgccgc ttcaggcctt cgatccgctg
     2101 gatcgaattc agaactgaag agcgcagggt gcgagctcag cggcgcgctc cggccgctga
     2161 gcggtggcca gcggagcgcg ctgctggctt acgaagtgtc cgtcgatcag agctggtatt
     2221 cgatcgtgtt cagggacttc acgcgcgcgt cccacttcaa ttcgcgaatg cggcccttga
     2281 tgcactccgc gagcttttcg ttcttgggcg tcgtcgtcac gtccacgccc accgcgcgcc
     2341 cttcccacac cgccacgcgc agcttgaccc gcgccgagcc aggcttgcac ggctcgtaaa
     2401 cgcccgcgtc ttgcaggggt ttaccgagcg tctcttggtc gatattccgc cgctcgaagc
     2461 cagcgggaac ggccttgatc gcttcatcca ccgacatggt ctcgctgaaa gccggctcgg
     2521 cgggttccgc cttttgctcc ttctttgccg cgctttcttc ttcggccccc gcatcttcat
     2581 cggtcttcgc cggcttcgta ctgacggagc tcgagctctc tgactcggtg ccgctggggg
     2641 attcctcgct cgaggcatca tccgccgtgc gcgctggctt ttcggagcag ccgaggagca
     2701 gcaacagcgc cgagacgaac aaaactcgcg acgtaacgca tacggaggcc aaacccgaca
     2761 tgcgccgcat gctactcgga cactcgacgt cgatcgagcc cgcaccggca gaacgaaatc
     2821 cgaacgtgag catcgacact ccccgtgatt ttaggtgcat tccgtcaccg ctgcaacgga
     2881 cacgcggcaa aaatgagagt gccattccga aaggctcaga ctcggactgg actccaacgg
     2941 ttgcatcacg gacgttgcac ggaactcgac atcaatcctc atcaggatcg ctacacattg
     3001 aattgcagtg cgaccgactc gaggtatcct ccctctcaca tgctgcccac tcccgagcgt
     3061 cgacggatag actggcgcgt gatttgcaac gaatcaccgt ctgaacttca actgtccact
     3121 ccgaccctgc ctgcccggca gctggcggcg ctcaccgcgg aggctctagg agatccatcg
     3181 ccggaaaccg ccgatcgtat tttgcgacat gcgatcgact tcgcccgatc gacactccag
     3241 atcgagcgtt gcgggatctt tctattggat tccgaaaatc aatggatgac cggcacgtgg
     3301 ggtacggact cgaagcgacg caccgtcgat gagcactcgc tgatttacga cttcggcgcg
     3361 ttggatcgcg aagtgttcga acgcgcgcgc gccggcttcc cgtggacggt ctacgagaat
     3421 tgtcctctca tcaacttcga ggatgacgag gtgggtgtgg tgagccgggg ttgggtcgcc
     3481 tgcaccgcca ttgtgggccc gcaagggcca ctcggaatcc tgttcaatga caccgccctg
     3541 agccacaccc ccgtggatga agcgcagcag tggcgggtcg tgatgttgtg ttcgatcctg
     3601 gccaacgccc tgtggcgttg tcggacgttc ttgttgccct ctgcgccgag ccgcttcgaa
     3661 gcacggccgg gtatcgtgcg cagcgtgact cggctgctca cgcgggatcc gacgttgagc
     3721 tgcgacgcga tggccaagca actcaacgtg agctcccggc ggctcgcgcg tatgttcaag
     3781 cacgaagcca agagctccat cgtcgactat cgaaacgagc tccgtttggc cgcgttcctg
     3841 gagcgcgtgg acagccaagg tggcaatctg ctccaggctg cgcgggacgc cggcttcggc
     3901 agctatgcgc agtttcatcg agtctttcgg gcgcgcttcg ggcgagcccc gagcgaatac
     3961 ttgctcgagc gcagaatgcc ggactcgaac gtgatcaact cgaccttgac gagccacgaa
     4021 gccagcgcgc acttcgccgc caacgaaggc atgctcacgg cgaaagccgt cggccaatca
     4081 tcggtaacga cggtcgaacc cggcgagtga gcccgccggg gaactcttgg acgcacgctc
     4141 gccccggagt tgcattcgac gttgcgtcca ccgaagcgcg cccagctttc gcgcattctg
     4201 aggctttctt catcagaggc cgtctacgac ccggcaagcg ctgcctgtct ctcgtcagcc
     4261 cggatggcga gcgcgagaag tgagagcgag aaatcggggc gcatgagagg accccgaacc
     4321 tttcgagcgc aaaactacgg ctgccctttc cggttgctgc cgccaaggcg tctgagcgtc
     4381 cagagactcg ccacgagttc cgcgcacgct tagttatcga atcgaaacat cgagtcgtag
     4441 agtccttgtc tcagttcgag tctcgtattc caaggagccg aaccgccgtc tggcgggttc
     4501 ggaaagagaa catgcgttta ggtccgtgga ttgcttattt cctgtttgga ttggccttgg
     4561 gctgcgatgc ctcgagcagc accccatctg caggttctgg gggcgccacg tccgcaagcg
     4621 gcggctctgt ctccggcggc tccgccaccg cgggcgagac gtcgggcgcc gggatgggag
     4681 gcagcgcagt gaccgggggc agcggcgcga cgggaggcat ggcgaccggc ggcatgacga
     4741 cgtccgcggg tggcggtagc ggcggctctt ccggtgcgag cgtctccagc ctttccggca
     4801 tcccgattcc accggtggaa gggaatgtgc cgccgcccac cggagccgcg agtacgctca
     4861 aaatcgtgga ctgggctggc ttcaaaggag cggtctctta caccttcgac gacaccaact
     4921 cctcccagat ttcgaatttc gatgccttga acgggctggg agttcggttg acgttctacc
     4981 tgcagaccaa caagacaacc gagctcgcga acgatgtctg gaagaaagcg ttggctgcgg
     5041 ggcacgaact cggaaatcac agccactcac accagcaagc ccccgcggag gccgccggag
     5101 ctgccgacgt cgatcaggcc caaggcatcc tcaaggagaa gtttggtgtc acggcgtaca
     5161 cgatggccgc tccgtacggc tcggccgtgt actcgaccat cgccaagacg cgttttttga
     5221 tcaaccgcgg tgtgtcgaac ggcctggtgg gtcccaatga cgcgagcgac ccgttcaatc
     5281 tttactgtta catcccgcct ggtaccgcga cggcagcgga tctcaacctc caaatcgacc
     5341 aagcgcgggc ggcggggaag tggcgcatcg tgctcgtgca cggcttcatc ggcggcacgg
     5401 acggcgccta cgaagccgtc aacgtgacgg agttcaccac cagcgtcgag cactccaagt
     5461 ctttgaagga catgtggatc gacagcctcg tgaatgttgg atcctactgg ctagcgcaac
     5521 gcgtgatcac cgcggtgacc cccacgacat ccgggtcgga caagacttgg acgtggcaga
     5581 tgccggagca tttcccaccc gggaagtatc tgcgcgtcac caccgacgga ggcacgctca
     5641 aacaaggagg cgtggccttg ccgtgggata gccatggcta ctacgaagta gcgctcgacg
     5701 ccggcgctgt gacgctgtct ccctagcgat gtctcttcag ctatcgctac gcttctccgc
     5761 cgcttggctc ctccgcggct gaggaaagag ggttgggata gagccgtacg ggccagaggg
     5821 gcaaaaccct tctggccccg ctgctcgcgc ctacttcttg gcggcgtgct cctcgaggga
     5881 cttgttcatc gcctcgagtg cgtcttcgta ctctttcttc aacgcgtcgt tgcgttgctg
     5941 catctccgta accgcgcgtt gcgcctgctc cacctcggcg tccaactgct tttgcgcggc
     6001 ctcggctgcg gctttgcgct tttgcgccag cgtcttggcg ggtttgctgg cgcccgcgtc
     6061 cgccaatacg gcgtcggagt ctttgatggt gcggtccagc gtcgagcgca ccgtcgactg
     6121 atcctcgagc aaggcttcga gttcgcgtcg atacaactcc aatcgcacgt gaactacgtg
     6181 gctcgcgtgg gtgatgtgat cggcctgttt gttgagggtg tcgaggttgg ctttacccag
     6241 tttatcctgg tgatcctcga tgtagcggtg cacttcactc tgcttgcgca atcgctcgtc
     6301 gagctgcttc tcgacgccac ggtccatggc tgatgccgcc actccgccca aatcctcact
     6361 gcagttcttt tgagttgcgg cgtagttcac cccgccggcc accttctgat gcaggccgtc
     6421 tttttcctcg tccatgaagc gcgtgacggt ctctgcctcc agcgccgctt ccgtgtaggc
     6481 gctcgacttg ccggtgctgt ccgcgagctc gtacacgtca cgcgtgctct cgagatcggg
     6541 attcttcagc gcctccgggt agctcttgaa ctcgccgctc tccgtcttgg ccttgccttc
     6601 ttcgtctgcg aaacggctgc ggatcgtgcc gaggcggctc gggtaatgtt cggcgtaacc
     6661 ggcctgttca cctgaagact cgacgacctt cggcggcggt tgattgcagg ccagcgccag
     6721 cgcggcgacg catccgacga gcagccatgt cccgagcgcg ggtgttcctg cggtcacggt
     6781 ggttccggag gggtgtttca tcgggcgcct cttttcttag gaactcgccc ggaagtcaac
     6841 tcgacaccgg gccggtccac tgtacgcctg acgtgcacca ctactcttca gctgtgcagt
     6901 tgggcttcaa cggcccaagc taatcccgag atcttccgcc gtgcggatcg cgtcgtgatc
     6961 gagcgggaca ctgcgtgtaa cgcggattgc ttcttcgatc ggaacagagg tcatgctggg
     7021 cggctgatac gaaaccatgt gaccgaagcg gccttccgcg gccaggcgta ccgccgcggc
     7081 gccatagcgc aatgcgagga tacggtcgcc cgcagtgggg cctccaccgc gttgcaagtg
     7141 accgagcacc aagctgcgtg tttcgaagcc ggttcgtcgc gcgatctcct cggccacgaa
     7201 agcgccgacg ccgccgaggg tgatttcctt gccggcttct cttgcccgca cgatatattc
     7261 gccgccaacc ggcttcgcgc cttcggcgac cacgacggtc gcgaagggcc gcccagtttc
     7321 ataacgatgt cgaatgctgc gacaaagggc gtctatatcg aaatcgatct ccggcagcag
     7381 aatcccgttc gcttcggcgg cgatgccggc atagagcgcg atccagcctg catatcggcc
     7441 catcacctcc acgaccatca cccggctgtg tgcttcggcg cttggctgca gtcggctcaa
     7501 cgcgtccgtg gcgaaggcca tcgccgactc gaagccgaag gtctgctccg tgctgccgag
     7561 atcgttgtcg atcgtcttcg gcacggcgat cacgcgcatg cccttctcca tgaagcgctg
     7621 ggcgatgcgt aggctgccgt cgccgcccac cgcgatcagt gcgtctgctc ccacttcgcg
     7681 atagtgctgc accacgcgat cactcaggtc tcgcacgagc tgcgggtctt ctgcggtggg
     7741 caccgggtat tgaaaggggt ttcccttgtt gacggtgccc aggatcgtgc cgccgcggtc
     7801 gccaatcccg cgcaccaaat cgcgagtgag cgggaccagg ccacttgggt actgatccaa
     7861 tagacccgag tagccgcgcc ggatgccggt cacccgaaat ccgcgccgca gcgcggagag
     7921 cgtgacggcg cgaatcacgg cattcaagcc tggcgcatca ccgccacccg tattgatgag
     7981 caaatgcata ggcggagcgt tctccgagcg tccgtgggcg tcaaatccgc gacggcacac
     8041 cgacgcccca gacggtcgaa aaccgtcacg gccacgggtg tatttggcta tcgaacgctg
     8101 ttttgcgtgt tcggcagcca tcggctgcgc gaagtggact ccgcccctcg acccccgagt
     8161 gaggtctcga ttcccccagt ccttccgcgg caatttcccg atgcccggga gatctccgat
     8221 cgtgtcgctc taaataccta cgtcgagagg ccgcttcggg cggatgtaat ccgcatcgag
     8281 ggcggctctc cccacgtcaa accgtcggtc tgcctcgtgc agcggcgctg atcgctagtg
     8341 accatgtgcc tattgttggt gtcagcgcgc gaacgaacat gtgttcagtc gcgattccag
     8401 gcgtcggatc atccaacgag accgcgcccc agcgttgcgc aacacgaggc aaacaagcat
     8461 tgagcttgcg ttgaaagcca gccaccatag tctgatgctc gagggaatcg tgacgagaat
     8521 ccaagacgcg cctacgaaag aagcgcctgc cgcctggggc actgtgcttc tggtagatga
     8581 cgagcccgag tttcttcgcg cgctagttcg atacctcagc cgcaataccc tgcgcgtcga
     8641 ggcgtactcc tcggcgcatg acgccattct gcgcattcgt cgcggtggca tagacgtggt
     8701 cgtgagtgac atttcgatgc caggtatgaa tgggctcgag ttgctccgcg ccatccgcga
     8761 gtacgagccc gatctacccg tgctgctcgt caccggattg cccgcgctcg agagcgccac
     8821 ggaagcgatc gagtacggcg cgctgaaata tctggtcaag ccgatcgatc ccgaagcgct
     8881 gtgcgcatcc gtggacaagg ccatgaagct atcgcgcttg gctcgcgcga agcgtgaagc
     8941 actagagttg atggggcgca ttggctcggc gtcggatcga gcggggctcg aggcggattt
     9001 cgatcgcgcg ctcgcgtcct tgtgggtggc gttgcagccg atcgtgagag ccaaagatgg
     9061 cagcgtattc gggtacgaag cgctgctgcg cacgttggag ccatcgctgc ccgggcctgc
     9121 cgaggtattg ggagccgcgg agcggctcag gcaactttcg cggttgggtc gcgtggttcg
     9181 gcagcgcgcg gtgaagccga tgctcgacgc cagctgcgac ttcgccttgt tcgtgaactt
     9241 gcacccagcc gatctgctcg acccggagct gtgcgcgccg gattcacctc tgtccgggat
     9301 ggccgcgcga gtggtcctcg aaatcacgga gcgcgccccg ctcgccgaca tggaaaacgt
     9361 agcccagcgc gtgcaggcgc tgcgagagct ggggttccgt atcgcgattg acgacctcgg
     9421 cgcgggctat gcggggctca cgagcttcgc tttgctagag ccggacatcg tcaagctcga
     9481 catggcgctc gtgcgcggca tcgatcaaaa gcccgtgcag cagaagctcg tgcagtcgat
     9541 gacctcggtc tgtaaagaca tgggcctgct cgtggtggcc gaagggatcg agacgagcga
     9601 cgagcgcgac accttgatcg ggctcggctg cgacctgttg cagggcttct tgtttgccca
     9661 gcctggccca cccttcccag tggcacactg gtaggtggga tcacgcgagc acgcgaagtc
     9721 ttcgcacgag tcgggacagc gccgcgcgtc atagggcacg tcttcaatgg ccgcgcgccg
     9781 ttactacgga tgcggcccgc gcttcgcgct ccgcaccgcc tcgggtcgtg gttactgctg
     9841 cagcgcgatc tgggacggtc gctgcgcagg tgccggaacg taagcatcca gcaaccggct
     9901 cagggtggtc gagctcaacg gcccctggaa cgtgacgcaa cggatgtgct tgaactcctg
     9961 agccatcgtg gcggcgagct ctgcgggcga gccacccatc aagtgttcac cgagcaacgc
    10021 cagctccacg cgggtgttcg gatcagcgag ccacaccaac gcttccagag ccgtgagtgc
    10081 aagcagcggg cgctcaccaa tttcggccaa agcatcggcg acggccgacg ccgccgcgat
    10141 gtccgaacac gccaccaaca cggaaggcat ggttgcgcgc tcgagctcct gcagcacgag
    10201 ctgctgaagg ccgtcctcga cgtcgggccg caggtgcgtg aacatcacgc cgatgctgcc
    10261 gttggagtat ggagtatgcg cggcacgcac cgtaacggcg ggcacacgca gtttgccgag
    10321 acctgcgatc tcgaggtgta ctcggcaacg catgcccggc tctagcggcg ggccgttgac
    10381 gagctgagca ccaccagccg ataagtcccg gacgacgtac tctccgacgc gggtctcttt
    10441 ggccagcagt gtttcttcga cgaatacggt cgcgaaagca gccacgtagc cacgcgggtg
    10501 ccgacgacgt tccttggatg acgggggttg catgggcggg atcgtgtcaa cgactcgacg
    10561 ccccttacaa cggttcggcc cacgtccgac ttgaggccat gagcgccaga atcgttgttg
    10621 ttcgcgcgag agccagtgtc tcgatcttga cgcggcttcg attcgcgctg gtgtctcgat
    10681 tcagaccgcc gcgggggaac atgtgatggc tagactctgc agcgctttgg cgcccacgaa
    10741 atcgggcggt tgacgctcga aagccaatcc tgcgccaggt cgagcctcgt ctgcgacacc
    10801 tggagatggg cccggcgtcg cgccgcgcgc tcgctaccta ataccaaggg gtgaacccaa
    10861 tgttccgaga cgagcctgcc ggcagtgcgc gctggcgaac tacttgagca gctgcagtgc
    10921 gagctgcggg agttggttgg cttgggccaa caccgatgcg ccagcctgag acaggatctg
    10981 attgcggctc atctccgcgg tctcttgcgc cacgtcgacg tcacggatgc gagagttcgc
    11041 cgccgccaga ttcagacgca tcgtttggat cgacgacacc gagacctcca agcgattcat
    11101 ggtggcgccg tatttggcgc gattcgtgga gatgatggtg agcgcgccgt cgatgcggcc
    11161 gagcgccgaa agcgagttgc cggtagtggt gcccgagacc gtggtgctgg cggacagcag
    11221 cgtcgtgagc ccgagaccgc cgaacgtcag ggtgatttgg tcactggcgg tgttgttcaa
    11281 gccgatctgg aaggtgatat cggtggcggt ctgcgagatc agcaaccgac cgttgaactt
    11341 ggcggactgc tgcatgcgcg ccatctcttc ttgtagcgcc gtgaactccg tctcgaggta
    11401 gccgcgatcc gtgtccgtca tgctgccgtt cgcgccttgc atcgcgagtt cacgcatgcg
    11461 gcccaggatg ttgctcatct cacccagcac gctctccgcc gtttgcgtca tcgacagtgc
    11521 gtcgttcgca ttgcgctccg caactacgta cgagcggatc tgcgtgcgca tgctctcgct
    11581 gatcgccaag cccgcggcgt cgtccgccgc agtgttgatg cgatagccgc tcgacaatct
    11641 agaaaagttc ttggtcaatg ccgattgtgt tttgccgaga gatttctgag tctcgagcga
    11701 ggaaatgttg gtttgaatcg tcatcggcat ggcacgaaga atctcctgta gaggcggcta
    11761 ggatgtgcgg cgggtgttgg gtttgcgggg ctcgcgtggc cttggggctg cacggctcat
    11821 gccagccggt cgcgccgcga aattgacgga cttggggtcc aacttccggc gaaccacaag
    11881 cccatgggcg tacgcggccg tcggcgtttt gacggcgcac tgccaaaaaa acggagcggg
    11941 ccgcgacgca cgtttgcaag cctcccttgc agcccggggc gactgtaggg gaggagatgc
    12001 taacgtcccg gcccccatgt ccgattggcc ccggttcgac ggctgtgaag tcgtcgagaa
    12061 gctccacaca ggtcctctct ccgagtctta tcgtgcggtt cagcgcccgc tcggtcgacc
    12121 tgttctgatc aaggccatcg gcgcgagcat cctgccttca tcaccattcg cggcatcgtt
    12181 ggaacgggaa gcccggctgc tggccgagct cgatcatccc agcatcgtgg cgctctacga
    12241 cttcgtgcgc cgcgacgagc gcatgtggat ggtgctcgag cacgtggacg gttggtcgct
    12301 ggaggaaatc gagaagaagg tcggcaagct cgatcagcgc ggagccctcg tgatcgcgag
    12361 tgaagtcgcg cgcgccctgc agcacgcgca cgagcgcggc atcttccacc gcgacgtccg
    12421 gccgcgcaat atcttggtct ccaagcgcgg ccacgtgaag ctcgccaatt tctcggtcgc
    12481 ctccgacgag cgcatggtga ccgcgccgga gctgctcgat cacggggcga gctacgtggg
    12541 gccatcgtac atgtcgccgg agcagatcct gggtgagcct tcggatccac gcagtgattt
    12601 gttctcactc ggtgtggtgt tgtacgagct tttggcggga gaacggccct tccaagcccc
    12661 ggacgagcgg aacgagtctc tgcgcatccg ctccgagccg ccgccgccgc tgcatcgccg
    12721 agttgcgcgc atctcgccgg gaatcgagcg cgtggtctgg cgctgcctcg agaagatgcc
    12781 gagtgatcga tttcagagcg cgggcgagct gctcgaagct ttggcgcagc tgcaacgtga
    12841 actcggcatc cgggacgagc cggagacgct gcggcgcgct ctggccgacg ccgggctcgc
    12901 aggggtgcct gcggctccgc gcagtgctcc ccgcgcggag ccgcctgcgg acaccaaggc
    12961 gagttggtca cgcggtgccg cggcgctgct ggtgttttcg gtggcgctca tcgcgggcgc
    13021 caccttgatc caaatcgtgt ctcggcgcgg agagcagaag gcgggaagcg tcggggggca
    13081 aacgcggctc gagctcgtgc ccgagaaggt ctcctacttg cgggtggtgg cgcatccgtg
    13141 ggcccacgtc gtcgtggatg gtcaacgcgt ggacacgacg cccttcgcca agcccatccc
    13201 gctcggctcc ggcacccatt atgtacggtt cgaacacccc aatgcgcccg tggaacggcg
    13261 aaccgtgcac ctcgcgcccg gcgaaacgct acttctggac gtctccatga aggtggtgcc
    13321 ggccgccgtc gcctccgagc gcccactcgt cagaggcacg gattccgctg cagataactc
    13381 gccctaacgc gctctcgcta cacgttgagt gtaaaggttc gagtgcttcg aacatcgagt
    13441 ttggaaaact ccaacatcgg gccaccagat ccgcgagccc cagcagcaaa acgacatcac
    13501 acccgcggcc gacctggccc tggctgagcc tccacgatgc gcgtcgacgc gggatttggc
    13561 gcagcgtgct gtgtctccgc tactcgccgg cttgcgcgtt ccgtggcatg ttcccggcaa
    13621 gttggttgat ggcgtcccac tggggacgcg ctgctcatga acgatttgac gtctgggtcg
    13681 aaccttggcc ggtacgagct cctcgttcgg ctcggacgcg gtggcatggc cagtgtgtgg
    13741 gtggcacggg aacattctgc gtcactgccg ggagaggagc gactcgtcgc cgtcaaggtg
    13801 atgttgcccg agctctcgag gcactccgac tttcgggcga tgttcctcga ggagggccaa
    13861 atcgtccgca gcatcgatca tcccaacgtc gtcaaagttc atgaggtggg tgagcaggat
    13921 ggcctgctct tcatggccat ggactggatc gaaggggact cgctcctggc ggtcattcgt
    13981 gaagccagaa agcggcgccc ggtgccggcc gagttggcgg tcaaaatcat cgcggatgcc
    14041 gcggcggggc tgcatgccgc ccacgagttg cgcggttggg atggggagct gcgcaacttg
    14101 gttcattgcg acgtctcgcc gcacaacatc ctggtgggat tggatggtcg agccaagatc
    14161 gtcgacttcg gggtcgctaa cgcgaccttg catagcgacg cggccaacga agagcgggtc
    14221 aagggcaagt tctcctatat gtcgcccgag caggcgacgg cgcagcggct cgaccgacgc
    14281 agcgacattt tctcactggg catcgtgttg ttcgagctca ctacgggcga gcggctgttt
    14341 cgcggcgaca cgccagagca ttccttgcag ctcgtgcgct ccggcgagat ccccgatccg
    14401 aggagcgtgc atccgggcta tccggatcga ctggcgcaga ttacgttgtg cgcgctcgac
    14461 cgcgatccgg cgcgccgctt ccagacttcg gccgagttcg cggtcgcgct cgagcgttac
    14521 ttggttgcag agcgggtgct ggtttcacac gcgagcgtgg ccaagttggt tcgcagggtc
    14581 attgggccga acatcgaagc cttgcgtcag aacttgcgtg aagccctggt tgcacgagac
    14641 gggcgtctac acgccgagtt gatccccgat ctgcccgcaa tgggcggcac tttcaccgac
    14701 gtctcgatca acttgccgca ctcccccgcg ctgttctcgc tttcgcggac gccgcagccc
    14761 caaacgctcg agcagccagc gccggtggtt ccgccgccga agcctcgtcg tcgcgtggga
    14821 gccctgctgt tcggcgtgtt gggcgtttcg gcggctacgg tggggggcct ttgggcaatg
    14881 caccgcccgg ggccttcgcc tttgcgtggg gcatcgggca agctggacga agggggctcg
    14941 ccttcgctcg tgaagcaaca ggactcgtct gttgcacact cggagtctgc cgaggccagg
    15001 gcgtccggcg tcgacgtcaa cagcttgcct ttgaccgcgc ccagcgcggg tgtgggcgtg
    15061 cgtccgccga gcgtcggacg cgtctccgcg ggcgtcgagc gtgcggccgc gggtgagcgc
    15121 gcggccggtg atcgcgcggc ggcggagagg gaacacttgg ccgccgagaa agatcgcgcg
    15181 cccgctgctt ccgcctccgc caagtccccc cattacgaag aggctgtgca cttggacgag
    15241 gaagtggcgc cggcacctcg cgcgagcacg gcgcccaagc ttccagagcc ttccgtcact
    15301 ccggcggtag ctccggcgtc tgggccgacg gggccgttca atcgcggggc ggcgctggca
    15361 cagctgaatg ccgcggcggc gcgcgcgtcc gcttgtggcc aaggcgcaag cggaagtggt
    15421 cgcgccaccg tgacgttcgc gcccgatgga acggcctcgc gcgtgtcggt gagcccgccg
    15481 ttcgccggca ccgcctcgga gggctgcgtg gacgccgcat tccggggcgc gagagtgccc
    15541 gcgtttacgg gcagctcggt atcgctaccg tggagcttcc gcgtccgttg aactcggcgc
    15601 tcccgtgctc ccgtcgtggc gtagggcgta ggcaagcaaa gcgagcgccg caccgctcgt
    15661 cattagccgg ctttcgcggc cgcggcttcg ggcttggctg cggatgcctc gggcatgcga
    15721 ttgcgccaga gccgccgctc gtcctcgaag attcgccaca agcgccgttg ctcttgtaga
    15781 aaagcggcgt acgtgcgggg tacgcgcggg tcattcaaga acgcgaccat gacgcgcagc
    15841 gcggcttccg cgtcgtgctc ggcgcggtgg gcctgcggga tttcgatgcc gaggcgttgg
    15901 ctcacttcgg tgagagcctt gctcttctcc agcttgtgca gctcgcgcgc ccacaccaac
    15961 gggtcgatcc actccaccgt gcggcgcact gcgggtggcg agagcgtgct gccacccggc
    16021 acgcgcgcga gctctgcgtg caggaccttg cggtcgtatt cggcgttgta ggcaagcggc
    16081 acgcagccgg ccatttccgc caaaatctcg tcggcaactt gcgcgaactt cggcttgtct
    16141 tttacgtcgg cgtcgctgat cccgtgcacc tcgaacgctt cctgggggat tgggcgttcg
    16201 ggattgatga gccatgtgcg cttgctcgcg acggccgtac catcccagat cacacacgcg
    16261 atttccacga tgcggtcctg caacggatcg cgcccggtcg tctcggtgtc gatggagacc
    16321 caacgcaaac tacgcacctc gcggtcaccg gcgaattcct cggcctgtcc acgcgccacg
    16381 accttcagca ggtgcgcgat accgggatag tggcgtccag ttgggaagca gccgagcgcc
    16441 tcggcatgtc tcatgggtta cccgccgctg gtttgggatc ggatttgggg gcgacgggcg
    16501 acttctccga aggcttcgac tccacaccct tgctgctggg cgccttggct ggctctgatt
    16561 tggaaggctc ggtcttgccg gggtccgctt tgaggccctc ggctttggct ggctccgtcg
    16621 ttttcgccgc ttccggagat ttacccacga tttcgatttc gctcttcacc cactcctcga
    16681 gctcctcgga gaactcgctc gtaggcggga atgggcgacc attgatgaat agagacggcg
    16741 tgctcttcaa ctgcaacgct tcaccctgtt tgcgatcacg gctgaccgcg tcggccgcac
    16801 tctcgctgtc tcggtcttcg cgaaagcgct tcaaatccag gccaatttct ttcgccaagc
    16861 gctcgatgtt ctcgggttcg agcgccgtct gatgctcgaa caacgtagcg tgcatgtccc
    16921 agaacttgcc ttggcgcaga gcggccaccg atgcgcgcgc tgccttctcg gcatgcgggt
    16981 gcatggtgag cgggaaatgc ttgaagtaga gcttcacgtc attggggtac ttcttgacca
    17041 agtcgtcgag caccgtgcgc gcggcgccgc atgccgggca ctcgaaatcg gcgaattccg
    17101 cgatcacgac cttgccctcg ctcgcgcctt tgctcggcgc accggcgaga tccacgctgc
    17161 gcacggcctc gggcgagaac cgcgccttgt acgcgccttc cacttgggcc cgcactcgac
    17221 cgcgtcgcgc ttgcgccagt aagaactccg ccgccggccg acaggctttg cacggtcgcg
    17281 cctctttcac acattgagcc aagctcaccg gttgatcagc gcacggggtg aggaactccg
    17341 tgacgtagct cgaccactcc gacttctcgc gcgtggtcag ggccgatgtg tcgataccag
    17401 gaagcgtcac cgctgctgtt tccgtgcgag ctgggcttgg ctctgcgcca ccttccccgg
    17461 gactacgaca ggtcgacagt tgggaactca gcagggcaga aatggcagcc gcgacggcga
    17521 ctttgaggct ccacatggcc cggcggagac tagctcgtgg ttcgaggctt ggcgacaccc
    17581 gaaacacgtg agtggcctcc gccctttggt ccgcacggtc ggagggcggc gagccgcgcc
    17641 cgctcgaaca cgggctcttc ggggacacgg cttggggcgg gcacgtctcg agccgaagag
    17701 ttcgtaacgg ccagggtttc tggtggcggg ttcttcgcga gctcgggcgg aggcagcgct
    17761 tcgggcgccc gaagccgccc tacgaccgcc caaacaccat ccgaacaccg ctccgcggtg
    17821 ccaaactgac gctccgagca cgagtttggc gaacggctgg cggttcaggg caggattgat
    17881 ggtagatcgg gcgcggcctg cccgatgagt gactccggtc cgtcttccta tcaccccgca
    17941 tccattccgc ccccgtcgac gctaccgccc ccgccgggcg tgcgtcatgt gatcgccgtg
    18001 gtaggcggac ggggcggggt cggcgcgagc agtctgagca tcaacctcgg cgtgtacctc
    18061 gcgcagctgg ggcgtaaggt tttgctgatc gacgccgatc cgacgggggc cgagctgcat
    18121 acgttgcttg gcctcgagct gcccccattg ccggacactg acgaagatcc agatgacctg
    18181 gagattgcgc cgctgcagac gcgggtgccc gggttggtct tgttgcctca gggatatcga
    18241 gtcggatcga ccttgccgat tcgccccggg cgtaagccgc gttgggcgcg gcgcttgcgg
    18301 cagctgaacg tagactacgt gctcttggat ctgggcgcgg gcaccgcgcc gtccactttg
    18361 gacctgttcc tcaccgccga ccagggcgta tgcgtgacca cgcccgagcc gccgagcgtg
    18421 gaggcgacgt accgctttct gagcgccgtt tttcttcgtt cggtgcgacg cagcttggtc
    18481 gaagaccggt atcgcatgcg cctcgtggaa cgcgcgcagt ccgaactcgg agcgttgccc
    18541 tcgcccctgc acctcgtccg caccatcgcg cgttacgatt cggcggtgag cgcgcgagcc
    18601 gccgccgaac tcgcgcagct gcgcccgcgg ctcgtggtca acaacgtgcg gctgcgaacc
    18661 gactcggatt tgggccccgc catggtggac atggcgcgcc gctatctggg cgtgaagctc
    18721 gactacgtgg gtcacatcga gcaggacgat gcggtgtggc tgagtgtggt gcggcgtcgg
    18781 ccgttgctga tcgacagccc taccagcaag agcgcacgta ataccgagcg gatcgcgcgg
    18841 cgtatcctgg cgctggcctc ggcgcggtcc gagctgcgct cggagccgcc gctgcccatc
    18901 atcgccgagg agccgaacct ttacgatgtg ctgtggactc accggggcgc gacggacgag
    18961 gaattgcgcc gcgcctacaa gcgtcagcgc gacatctaca cccccggtag cctcccgctc
    19021 acctcgctgc tcggtgaaga ggagctgaag cgcgagcagg cgcggatcga agaggcacac
    19081 gagaccttac tcgatcccct ccgtcggaaa gcctacgatg cgtccgtgtt tcgcgaggac
    19141 acgagctctc cgccgcgggc acccatgccc cgcgatgcgg ctttggaggc ggagcgcgcg
    19201 ctgctccgtc aggagctttc gcgtgaaatt agcgcggaaa ccgagttcac ggggcgactc
    19261 ctcggcaaag tgcgcgaagc ccagggcatc gagatcgagg atatcgccaa gcacacgcgc
    19321 atctcgatcg cgcatctgaa agccatcgag tccgaggcat tctcggagct cccggcgctg
    19381 gtttatactc gcggcttcgt gcagcaactc gcgaagtttc tgaagctaga tcccacgcag
    19441 gtgtctcgca cgtacctgca tcgtatgcgt gaggccgtga gccggcccga cgaggaaagc
    19501 tcgtcgtgaa cggcgcgcca cctcggccgc ggcgcccgag cgatcgagcg ttcgggtttg
    19561 cgctgttctg ggtggctctg ctcccgcgct tgtacgtcgc ggtggcgtgg atcaaggagc
    19621 ccgtatggga cgggcactac tatcacttcg gcgcggagcg catcgccgcg ggcttgggtt
    19681 actccgagga tgtctgggtt cgcgggcaac tcatctggaa gccttgggtg cactacccgg
    19741 tcgggtacag tgcgttgctc ggcgcgctgt accggacgtt tggctcgagt ttactcgtcg
    19801 cgccgctgct caatgcgctg ctcggcgcgt tgctcgcggt gacgacgttc cacggtgcgc
    19861 gcgatttctt ggggcaacgg cgggccgcgc tagcggccgc cctggtcgcg cttcatcccg
    19921 ggttaatcgc ttatacgcct ctggtgatga cggaggtgct cgccgcgttt ctgatctcag
    19981 ctctcgggct gagcctgctg tcgcgtcggc gctgggcggt accggtcgcc ggtgtgttgc
    20041 tcggggcgct agtgttggta cggcctgctt cgctcgcgct cttggcgttg ctgccgttct
    20101 tgcgtcacgg aggggtctgg acgcgaattc gcgacagcgt cctggtggtg ctcttggccg
    20161 ggctatgcgt gatgccctgg accgttcgca actgccaacg gctcgacggt tgtgccttgg
    20221 tttcgaccaa cggcggctgg aacttggcga tcggagccat caccgagagc gggcgctttc
    20281 aaacgctgcg cgctagcgac ggatgctcgg ccgtgcgcgg gcaggtgcac caagaccgct
    20341 gttgggccga gctgggtaag cggcgcatcc tcgcggatcc gatggggttc ctgcggcgag
    20401 ttccgcgcaa gctggccgag acgttcaatc acgagtcgtt cgctttcgaa tacctgcacg
    20461 aagcggatcc catccgttgg tcggaagccc gtcgggttgc gggccgcgag ctcacgagct
    20521 ggtaccaccg gctgctgctc ttgattgccg gcttcggggt cgtggcgttg ccgcgcgagt
    20581 ttcgtcgtga ccgctggggc ttcgcggtgc agtgcgcgtt actgttgacc ttcggcgtgc
    20641 tgtcgggccg cgcgctgatg ttcgatgaca aaccgttctt ttggctgccg gtgttcgcgg
    20701 ccgtagttgg gctgcttccg ttgcccggac gacccgcgac ggggccaggc gccttgtatg
    20761 cggcgggcgt tctattgtcg gtggtgctcg tgcacgcctt gttctttggc gaggatcgct
    20821 atcacatggt ggctagcccg atgctctgcg tgttggcggc tgccgctttg cgcccgagcc
    20881 gtggtcatct acgctcgagc gccactactt ggtcagctcc agaaaacagg ttgttgaaca
    20941 cggtgccagg ctcgagcggg gtgaatgaca tttacccggc caagctgctg cgctgagatt
    21001 cctcgctgcc gccgcaactg aagtcgtcaa agtcccggtg attgccgatc gaactggccc
    21061 ggctggtctc gggtcaacgg ctgctctacc atggttcggc gatgcgcacg cccaccgaga
    21121 acccgttgca atactgggcc gagctggcgg agaaggagct acgcggagcc aagccggaga
    21181 gcttgaattg gaatacggcg gagggcatcg cgctgaagcc gctgtttacg cgagccgacc
    21241 tggagcagct cccaaacgtg gataccgttc cgggcgcgtt cccgttcgtg cgcgggccgc
    21301 gcgctaccat gtatgcgggg cggccctgga cgatccgtca gtatgcgggg ttctccaccg
    21361 ccagcgagtc gaatgctttc taccgttcgg cgctcgcttc agggcagcgg gccctcagcg
    21421 tagccttcga tctgccaact cacaggggtt atgacagtga ccacccccgc gtcgcgggag
    21481 acgtgggaaa agccggcgtc gccatcgatt ccgtggagga catgaaggcc ctgttcgagg
    21541 gcatcccgct cggtgaaatg agcgtctcga tgaccatgaa cggagcggtt ttgccggtgc
    21601 tcgcgagcta catcgtggct ggtgaggagc aaggggtggc gccggctcgg ctcaccggaa
    21661 ccattcagaa cgacattctg aaagagttca tggtcaggaa tacgtacatc tatccgccgg
    21721 gcccctcgat gcgcatcgtc gcggacgtga ttcagtacac gagccggcac atgccccgct
    21781 tcaattcgat ctcgatctcc ggctatcaca tgcacgaggc gggggccacg gccgaccagg
    21841 agctcgcgtt cacgttggcc gacggcctcg aatacgtgcg cgccgcttgc ggcaagggtc
    21901 tcgccgtgga cgactttgcg cctcggctct cgttcttctt cgcgatcggc atgaacttct
    21961 tcatggaaat agcgaagctg cgggcggcgc ggctgctctg ggcttcctgg atgaagcggc
    22021 tgtttgcgcc caagctcgcc gagtccatga tgctgcgcac ccactgtcag acctcgggcg
    22081 tgagtttgac cgagcaagac ccacacaaca acgtggtgcg tacggccctc gaggcgctag
    22141 cggcagtatt ggggggcacg cagagcttac acaccaactc gttcgacgaa gccatcgcgc
    22201 tgccgagcga gcacgcggcg cggatcgcac gcaatactca gctcgtgatc gagcacgaga
    22261 ccggagtgcc gcacgtcatc gatccgttcg ggggctccta cttcatggag tctttgacgg
    22321 cacagctctc ggccaaggcc tcgagcttga tcgaggaggt ggaggcgatg ggcggcatga
    22381 cccgcgccat cgaagccggg gtccccaaat tgcgcatcga ggccgcggcc gcggagcgcc
    22441 aagcccgtat cgatcggggc gtggacgtga tcgtgggggt caaccgcttc aagaccgatg
    22501 ccaatgctcc ggtcgaggta cgcgtggtgg ataacgccgc ggtccgcgag acacaaatcg
    22561 ctcgcctgaa cgagctgaga cgcggtcgca acgaggccac caccaccgcc gcattgaacg
    22621 aactggccga agctgccgcg cgatccgacg ctaatttact agaagcttcg attacggcca
    22681 tccgcgcgcg cgctacggta ggggaggtgt cttcggcgct cgagcgagtg tttgggcgtc
    22741 atcgagcgga ggcgaagctc gtgagtggtg tgtacagtca ggcctacggc aatgagcaag
    22801 agtggctcga tctccgcgac caaatcacgc aattcgccca acgccacggg cgccgcccac
    22861 gtatcctcgt ggccaagctc ggccaagacg gtcatgatcg cggcgccaag ttgatcgcaa
    22921 cggccttcgc cgatctcggc ttcgacgtcg acgtagggcc gctcttccaa acaccactcg
    22981 aggccgcgcg ccaagccgtc gacaacgatg tacacgtgat cgggatctct acccaagccg
    23041 ctgggcacac gagcttggtg cccgagctgt tgcaggcttt ggagcagctc gaggcgcgcg
    23101 atatccgcgt ggtatgcggg ggcgtcatcc cggcggctga tcgctcgaag ctcttgagcc
    23161 aaggcgtggg tgcggtattc ggccccggta caccggtatt agaggcggcc cgcgccgttt
    23221 tgaagctgat tacatgactg actcggactc ctcggttcag gagctcgcgc ggcgcttgcg
    23281 cgcgggcgaa cggcgagccc tggcgcgcgc cattacgctc gtggagagcg cacgcgtcga
    23341 ggacgatcgg cgcgcggcgc tgctgatgag cgaactgttg ccgcacacgg ggggcgcttt
    23401 gcggatcggc gtgagcggcg cgccgggggt gggcaagagc accttcatcg aggctttcgg
    23461 catgcatctc gtgaagctcg gacatcgagt cgccgttctc gccattgacc cgtcgagcgc
    23521 cctgtcgggc ggttcgattc tcggcgataa aacgcgaatg cccgagcttt cggcgtcaga
    23581 tcgcgtgttc gtgcgcccga gcccctcggg cggctcgctc ggcggtgtgg cgcaacgaac
    23641 tcgcgaagct gctctgctct gcgaagccgc cggctacgac atcgttttaa tcgagacggt
    23701 gggcgtcggg caatcggagt tcgcggtcgc tcacatgacc gacacgttcc tgctcttggc
    23761 cttggcgggc gcaggggacg aacttcaagg catcaaacgc ggaatcttcg agctgattga
    23821 tttgctcgcc atcaccaagg ccgatgggga gaacgtgagc gcggccgagc gggcccgcgc
    23881 tgagtacgcg tcggcattgc ggctgctcca ccaaggtctc cgcgggacgg ttccggaagt
    23941 gctgtgcgtg agcgccgtcg aacagcgcgg tatcgagacc gtgtggagtc agatcgagcg
    24001 ggcggtcgaa cgagctcgcg cgagcggtgc cttcgatgcc aaacgcgcgg cccagcgacg
    24061 cgcctggttc tcggagtgcg tagagcagga gctacaccgc aggttctttg ctcgacctgg
    24121 tgttcccgag cgtttctcgg gcctgcaggc acaggtgttt cagggcgacc tcttgccccg
    24181 tgaggcggcc cggcagctct tgaacgacgc gatctgaaag agagaggccc gcggcaacag
    24241 gtgcgacgcg ggcctcggtt tagcttcgag cggcgccgga ttcgaccgaa tccgcgccgc
    24301 tcgtctcgtg atcaggcggt cgagtcagtt gctgctcacg gtcgggttgt ccgcgtacgg
    24361 gcgagcctgc ttgatgtaaa gcgacggctt ctgtccgggg gcggcctcgt cgtcgaactt
    24421 gaactcgcaa tccatcgcgt accaagcgtc cgggctggct tggtatgcct tgcggaagcg
    24481 ctcgtgaatg gagttcagcg cttgcccgag atcgtaaatc tgacgggcgt cgagcacgtg
    24541 ctcgccttcg ttcaccaagt tcgagcgcgt gtaatagatg acgggttggt tggggaacga
    24601 atactggtag aggaatgagt cgctcgtgac gccgggtggt ggatgcacga cctcggcttg
    24661 tccacccttc tgcacgttta cgtagaagcc cggcgcgttg ccggtcggat cgaagatgtt
    24721 ggccgtcacc gccacgccgt tggcctcctc gtccgggaaa ttggtgtgga cgagcaacgc
    24781 catcgcgacc ttggtatgcg cgatgctgtg cagcttgcgc tcctcgtacg tacgcaagtt
    24841 ccaaaccgtg gcccaggtct tcttgattgc ctcggccgcg gcggcgatct ggtcgcctcc
    24901 gaactcggcg gggtcacccg tgtgggaatc gtagcatccg gcgcaaggga agccgtcgag
    24961 gtcctcggcg ttggtgctcg accggaagcg aatcgccttg ccgggatagt ccttgtctag
    25021 cttctctttc agcttggtct tgagctccgt cccgatgtcg gccttcatca gatccgcgcg
    25081 cagctcggcg agcttttgat cgcgcaccga atcctgcccg gtgaagttcg ggtcttgttc
    25141 gagttgcgtg actcgatcgt agaacccgtt cgccttcatg aaggcgtcgt agaagtaaac
    25201 cgggattgcg aagcccttct tgtgcggcac cttgggatcg gtcgcgaaca ccgagtagtt
    25261 ggcggctttg gccccgaaga tgcggatcga ggccttgagc gcgtcgagcg tcacgtacgg
    25321 ggcgacgtcc gtgtgctcgg ttacggtctc gacgtctctc agatcggtga cgctgatgtc
    25381 tggatccggc aaggtgacgg gcgcgggctt gtgctgctcc caccaagcat tcgactcttc
    25441 ctgggtgact tcgaccacct tccactcgaa ggagcccacg gtcaggcgca cgtgtttgcc
    25501 gaacaagggg gtcaagagcg ggttggtcgt ggctttgcgc agccccatgt tcggcgtatg
    25561 ccgcgttcgc gcgagcacat tgatgtgaga gagcggcgtc tgaaactgct cggtgatgat
    25621 gccagcggtc ggtgagatat cgttcggaat ggcatccaac accacgatgt cgcgcgcgga
    25681 gacgtagatg ttgtcgaggt ccaccgatct gaccatcttg agcggcccca cgacctcacc
    25741 caagttcaag ggttgatagt cgatggacgc atacagttcg tcggtcgtga cgaggggtac
    25801 gccggtggcc ttgcgcgcgc tcgcgtccac cgcttcggac gtcgggtgga acttgagcac
    25861 cggcccgtag taggcgttgg ctttgaccgc gtcgaacagc gtcttgatca tgtccggcgt
    25921 ggacgtgtcg tacggcgcga tctcgagcgc ccaaatcttg ggtgcctcgt agtacgtgac
    25981 cgcgccgagc atgaagcgcc gctcggccag aggcaacgtg tactgcggat tgaaatccga
    26041 cagcgaaccg atcgtgagat tcggcggttt gctcaggttc gccttcgcga actcgtagtg
    26101 gatcgggtac ttgttgctgt tctggaaata gagctggttc tgatccatca gatccaacac
    26161 gaccttcccg gaacgtgcgc ccgggatgct cgcgtcgagc ggttgtgaag ccaggatatc
    26221 gaaatccgct ttgcagccgg tcttcctcac gaacagcgta ttcgcgccct cggttgcttc
    26281 acacggccaa gtcagctcgg ttgccggcgg catggtcgtg gggggcatag atgtccccgt
    26341 ggcagagggc atcgggctag tccccgtcgc tgacggcatg ggcgtagtcc cggcaggctt
    26401 gttctccgat gagcagccga ggctgccgaa caggcctccg aggctcaccg ccatcggcac
    26461 agcaaacgac gcaagaaacg ggcgaaaacg agaaggaggc cgacgtcgct cacgtccctc
    26521 cgagatggcg gcagaatcga ttcgaatctt ctgtatcatg aggcagcctt gggcttcgtt
    26581 ggacccagct ctggtagtaa cggcggcggc cgaaaccata ccggctgttt gacggatgtt
    26641 cgactttggg atgacaactc agccccgcac cttgtgagcg gcatcagtcg attcccgcca
    26701 aagggcaata atcgattctg agtgtgcgtg cttcgagggg gcgctgttac cgtgccggtt
    26761 tctgcacggc gcgagtgtct ctccgcggga tgagatcagg agtgagaggg cgagttctgg
    26821 gcgggcttgg cgaggcgagc gctccaacct cgatcgggca gagcgccagc ctgcgaattg
    26881 tgaagatctc ggcgagctag agcaaggaac gcgtcggttt gcctgagtcg taccaacgcg
    26941 cgctgccgtg gagtacgtaa ttggcttcct ggggcccgaa ggtgcctttg tcgtattcgt
    27001 agattggagt cacgtctccg agtacgggct ccacgatccg ccaggcggct tcaacgctgt
    27061 cctcacgagc gaacaggttc gggtcacctc gcatcgcgtc accgatcagg cgctcgtaag
    27121 cgcccatctc gtcggctcgt tggttgcgaa ccatgagctc cacgctctcg cccgccatcg
    27181 catcgccggg gcgcttggca cgggctccaa tcgcgatcgc gatatcgggg cccaagcgga
    27241 accgaacgta gttcgtgttc gcgggcaccg gctccggaaa caccatgtgc ggcggacggt
    27301 ggaagtccac cagaacttcg gtggccgtga ccggcatatt cttgcccgtt cgaatgtaaa
    27361 acggcactcc cgcccagcgc catgaatcca catgcaaccg aagcgcggcg aatgtctcga
    27421 cggtggagtc tttggcgacc ccttcctcct cgcgataacc cttgtattgc ccgcgcacca
    27481 cgttctccgg agagagcggt cggatggcct tgaacacctt ggttttctcg tcgcgtagcg
    27541 cttcgcggtc gcccgccgga ggcggttcca tcgccaggaa cgcgaccacc tcgagcaagt
    27601 gattttgaac cacgtcccga attgcgccgg cctcttcgta gaagcgcccg cgccccacga
    27661 cgccgaaatc ttcagccatc gtgatctgca cgctgctgac gaaatttcgg ttccagaggg
    27721 gctcgaggaa cgcgttcgcg aaccggaagt agagcaggtt ttgcaccggc tctttgccga
    27781 ggaagtggtc gattcggaag atcgaagact ctccgaacac gctgtggagc acctggttca
    27841 agtggcgtgc cgaggccaaa tcgcgcccga atggcttctc gacgacgacg cgcgcaccct
    27901 ccgagcagcc gctccgaccc agaaactcga ccacgttggg aaacgcgctc gggggaatcg
    27961 agagatagta gagcggccgc cgagcgtctc ccaaggtctg ccgcagcttg tcgaagagct
    28021 cgaccgtgcg gtagtcccca tctacgtagt gcaggttgtc ggccagcgcc gagaaggcct
    28081 tttcgtctag gccattgttg ttcgaaaccg aggcgcgcac tcgggaccgc aggtcctcca
    28141 agctcgatcc gccacgcgaa actcccacga tcgggaatgg cgcgtgccct cggcgaagca
    28201 tcgaatacag cgcgggaaag atcttcttga acgccaggtc tcccgtggcg ccgaacagca
    28261 ccaacgcgtc cgaagcgagt tccgattgca tcatgtcgac ttcttttcgt gatggccacc
    28321 gaagccgagc cgcatggccg agagcacacg gttggcgaaa tctccctgca tgcgcgacgc
    28381 gaagcggtcg aacagcgagg cgctcagcac gtgcgccggt accccgatgt cgattgcggc
    28441 ctgtacggtc cagcgccctt cacccgaatc cgaaacgtta ccgccgaact cctccaggcg
    28501 cgggctcttg gcgagcgcgt cggccgtgag atccaggagc caagagctga tcacgctgcc
    28561 gtgcttccag agctcggtga tcgcggccag atcgaagtcg tattggtagt gctcggggtg
    28621 gctgagcggc gtcgtttccg cgtctttctc gcgggtttgg tggccggcgt tggcatgacg
    28681 cagaatgttc aggccctcgg cgtaggctgc catcaagccg tactcgattc cgttgtggat
    28741 cattttgacg aagtgcccgg cgccgagggg gccgcaatgc aggtaaccgc gcgcggccgt
    28801 agccatggca ttggtgtgag aggcgtcacc ctgagggatt gcggtggtcg gagcgtcgtc
    28861 cgttgcgccg ggagccaacg ccgcgaagat cggatcgagt cggttgaccg cggccgagtc
    28921 acccccgatc atcaaacaat agccgcgctc gagcccgtgc acgccgccac tcgtacccac
    28981 gtcgaggtag ccgatcccgc gcgccgctag gtcgtgggca cgccgcagat cgtcgtgata
    29041 gtggctgttg ccgccgtcga tcacgacgtc acccgactcg agctctgcac tgagcgcgcg
    29101 taccgtgctg tcgacgtgtg cagccggtac catcagccac acggcgcgtg gcttttgcag
    29161 ctttttcgcg aactcggaaa gagtggtcgc gccttcggcg ccctcgcgca cgagcgtcgc
    29221 gatggcctct ttggacacat cgtaaacgac cggtgtgtgc ccggcgcgca tcaggcggcg
    29281 caccatgttt gctcccatgc ggcctaggcc gatcattccg atctgcatgc tggctccttc
    29341 gtatccatcg aggcgctcag gcttgccgag attcgccgcc ttgtccacgt aagagacgcc
    29401 gcgactcgaa gcgtgagcgc gtggcgtttg ggtgcccggc cgccctcgtc cacacttgga
    29461 gggcttgagc tgagagaatg caccaagcgc ggctggttct gagttcccgc aggttcggga
    29521 tgtttctggg acctgcgtct gctcgctcgt cggtcaaatc ggcgaaatcc cagagtgttc
    29581 accgggcgcg cggccgccgt gtcggacgag cccacgtgaa agctccgccg aacgacgaac
    29641 tcgaacgcca gccgaggtgg cgccttgcct cgttccccgg ccgtcgaacg gcgatgctat
    29701 tctggcgcca tggcgacgtg caaaggctgc ggtgaagagg tcgactcgtt ggtgtcggtg
    29761 aaggttggcg ggaaaaccaa gaagctctgt gaagactgcg ccgatcgcgc ccgcgaagaa
    29821 ggggagatcg cggaagcgag tgagagcgtg gtgcagaaca tgatgggttt caaaggtcgc
    29881 cggtgagccc ttcttgaagc cgctcatttc aggttgggtc ttgctgtgtt tcgtggtgct
    29941 ctcgagttgc cgcgagcctg cggcgagcca gacccgtgcg ggtcggcgcc cggcgagtgt
    30001 cggaacgagc tcgagttcta gcctgcccgt gaccgccacc gaggcggcgc aggcagccgc
    30061 cacaccgggc gcagttcaaa acgcctccga ccggggccga actcagctga gtgcgattcg
    30121 cgcggatcaa ttgttagcca cgattcgggg tttcggcgcc aaggccacgc tagtcaatgc
    30181 gtgggcgagt tggtgtgggc cgtgccgcga cgagttgccg atgttggaga gcctggcgca
    30241 aaacttgtct cgcgatgacg tgcgcctggt actcgtctcg gtggacgaag ccgaggatgg
    30301 cgccaaagca gaggccttcc tgcgcagtgt cggaaatcga tcgcccgcgt acctcgcagc
    30361 gcctccgctt tccgagttca aggtcggaat gaatccgcgt tggcccggca tgattccggc
    30421 cacgttcttg ttcgatgcgg ccggcaaact ccggtatttc tggggtgggc cggtctacga
    30481 acacgagatt acgcaagtca tcgaggggct gcttgcgggc aagctcatcg atggtcagtc
    30541 cgacttcacg ctggcacccg ggaagaccga agcgggacac taatcaggag acaacgggtc
    30601 ttcgctgagc caggcccgac ctcacgaact gctgattcga atcctcgtgt tgcgagccta
    30661 cagtgggttg ccccgaaata tcgaaagcgg cctcgagatt cgcggccgat tcgatcgctc
    30721 ctgcgtcgac tcgagattgg cagtcgctcc aacgcgaatc aatacgagcc caggcaaccc
    30781 cacgaagatg gaatgaatga aactctccgc tcgcgcggtt tcagtcgacg cgcccgcgcc
    30841 cgcgctgcag gcgcatgcta ggctcacgac tccccgccgc tccatcacgc atatcaggta
    30901 gagctttatg tgcgccatgc ccatgactga catcgactat ccggggcgat ccggtgcagg
    30961 aggcttgtat cggttctgcc gcttcatgtt gctacctctc ggtcttgtag gcgctgtgat
    31021 cgtctcctac aggaacgact ggctacgcga gggggcacgc aagttcggct acgagaagca
    31081 ttatctgtcg ctcgagcaga gcttagtggg cactcccggg tggggtacgc ctcgcgagct
    31141 ttctcagagc ctggaggcag aacaggcggc actctcgggt tcagtcgaag ccaactcgaa
    31201 ccggcgctcg gcaggttcgt ccagctccgc gggtgaccag ccagatcggg aggacagtga
    31261 gggcacggct gcagagcgtg cggttgcaga gggcacggat gccgcagggc agcggcacgc
    31321 cgattccgag caagcggcgg agctggagca accggccaag gatcccgggg cgcgccggtc
    31381 cgcggcagag agcgtttcgg ctcagtccga agccacgacg cggcagtacg cggtggccgc
    31441 gcaggagccc gaacctagct ccccggtaca gcgcgccgcc ttggcccaaa gcgccgctgt
    31501 gttgtcccgc gcttccggca accaacgcag ttccgatgct caacgcagtt ccgaactcca
    31561 gcgcagtcat gtgactcgag ccgaagaggc cacggtcgcg ccgcgagtgg cggccaagac
    31621 gcgggtcacc cgcgaggtca tcgaagacga ggatgatgac aagcctgccg cctcctccgg
    31681 tccttccaag aaggcactgg agttgatcgc aaagcgcgcg gctgcgctgg ccaagagcgg
    31741 tgagagcagc gacaaggtcg cgctcgcgga gaagcccaag ccagtcgaga agcccaaacc
    31801 cgagaaggcg gccaagccag agaaggccaa gccggcagct ccgccgcccc caccgctggc
    31861 ggacacgccg ttgaacgcgg cgattcgcgc ctcgatcgtc aacggcaagt gaggcgctct
    31921 tcagcgccgc atgtgttcgc tatccccgcg cgtaacatac tcgccgcgcg ggaaatggcg
    31981 gtagtagtcg ccggaaagaa cgggctctcc gtcggcgggg tcgcggtcgt cacgtagccg
    32041 tccaatgacc cgcaccaaag accccggttg aacgcgccgt cggcccaggt cgtcctcgcc
    32101 gagcagcttt acctgcgcat gaatcacggc gtgctcacgt tcaccaatcg tgacgcggca
    32161 gctctcctca tccgcatcgt cgcacaggtt gcgatcggcc aaggttcgaa ttcccaaggt
    32221 cagctgggcg agcccgccgg gcccatcttg gcgattcttg acgatgccaa acacgctgac
    32281 cggggtcttc ttccagtctg ccggcgcacg ctgcgccata accggatcgt actcacgcgc
    32341 cgtctctgcg gcgtgctcct ccgcgtccaa cgggctgtat acccggctgt aaccgtacgg
    32401 cccgggggag taacaggcca caacgagcag agcgagcggc agatacttca tggctcatga
    32461 ttcctgcgcc cgatccagta tcgagcagca tttccagccg tctcggagcg acagcggcgc
    32521 cggactctac cgcagaagcc cgagcgcgat ctaggctcat gcggtcgggc ttgctcggcg
    32581 ttgcgccatt tccctgagca aacgcagcgc gagttcgagc gcggtccacg cgagcagcac
    32641 ccacaccact tgcccggaga cgtcgacttc tcttacgccc acgcccgcgc ttcggctcaa
    32701 ggcctgcgtt ggcggcaaag cgggctcggt caagagctca ctcgcatcca gcgagaccac
    32761 tcgctcttcg gttccttcgt gtgtgcggac gcggtacacc cccgcgagct cgggcgtgaa
    32821 gagctgtcga cgcggatccg tgctcaaagc ctcgctcgac agacgcttgc cgctcggatc
    32881 gctgacctcg gcctgacttc ccgccgcgaa tcgaaacggc actccgggga cgctgctcgc
    32941 gctgctcgct cgttcgtgag cggcttctac gaactgatcg agcagcgcca agaaaccggg
    33001 acgcagcgca aagtcgctga gctccggtga acttggtaaa gccaacgtcc acacgagccc
    33061 gagcccgaac tcacgctcca acaagaaagg ggcaccgtca tcgaaacgag cggcgactcg
    33121 cgattcgacg ctggcctcac cggccaaatg cacccgccct tggggcgcga gctcggccaa
    33181 ggcggaagct tcggggccta gccaggcgat ggacgctgcg tccacgcctt tggcgcgcgt
    33241 cttctcccaa ggcagacctc cttgagcaaa cggctcgagg gtggctccaa tcgagcgact
    33301 ctcggcgctc ggaccaagca acgcgagcgc caccccgccg cgcttgagcc acgtctcgat
    33361 gccgtctcgg acttcgggtc caagtccgtt cgggtcatcg agcacgagcc cggcgaagct
    33421 cgcgagctcc ttgggttcct cgggcaagac cgcgagcggg cgcacccgca agtcactgcc
    33481 cagtgcttcg agcgcgctct ccaccagccc cggaccgaat gcgtctgcgt gagcttcttg
    33541 tgcttgcgtc caaacgccga tgccgagcgc gctgctcccg cgccgaattg cgccttcgtt
    33601 gtcctcggaa atcgcgtcct cgccgctcaa tttcacgcga cgtgcttgcg ccggggccgc
    33661 accgaatcga agggtctgca agcctgggcg tgcttgcagc gcttccgtgg ccgcggctgg
    33721 ggccgcgctc gcggcagcgc cgctcggctc tcccgctacg aggctcactg tgcggccttg
    33781 agctgcccgc atcgaggtgc aagcgacttg cacgctcaca ccatgaccat cggattcggc
    33841 gctcgcgatt ccgcagttct ccgttcgtgc cgcgagctcc ggcagaggca cccagagttc
    33901 tcgcgcttca tctccgatct cgcccgcgaa gtcgctcaac agtgccagct tacggtcacg
    33961 ttgcgggagg cttgccagca tgctctgtgc cagcttcacg gcccctccga gttcggtgcc
    34021 gcgatcggtg acccgcaatt cctgcagcgt gcgtcgggcc gccgcgaggt cgcgggtggc
    34081 cgcgagcgcg atgcgagccg gcttgccggc gagcacgacg gtgacggcgt cgccgctgcg
    34141 cgccgtaccc acgagctcga gcgcggcgtt ccgcgcgcgt tcgaagcgcg tacccccgtc
    34201 gggcaaacgc gctcgcatgc tctgggaatc atccaacacc agcgcgagcg cgaccgaagc
    34261 tccgcccgat cgatccagcg cgaggcgcga acaacgcagc aacggcgtgg ctcccaacaa
    34321 cgccagcgtc gcaatcagca acgcgcgcaa caagaataga aagcggtctt ccaggcgcgc
    34381 gcgttgccgg gccacggagc gtgccgccgg cacccaggcg gcaggaggaa agacgagctc
    34441 ccgagctcga ccgcgacgga gcaggtgcgc caggaagggg gccgcgacga acgccgtgat
    34501 ggcgagggct gcgacgacga cgaagctcat gagttttgac tcagactttc cccggcccgc
    34561 tcgattccga gcaggacttg ccgcacgatc gcgaccgggt cgtcctgcgt actgcaggcg
    34621 atgaagcggc caccgcgtgc gatgactcgc tgctcgaagg cgcgcgtggc gcgctcgagc
    34681 gcctccagat acgccgcgcg caccggatcc gcatccgtct cgacgacggt gctaccctcg
    34741 gccgcgcgca acgctatcgg accgtgaaaa gggaaggtga gctcggctgg atcgagcacg
    34801 cgcaacacga tcaaggctcg tccagccgcg ccgagcccgg acaacaactc ggccgcggtt
    34861 tccggtaagt cgaccaaatc gctggccacc agcacgatgc tcccgcggcg cgcataacgt
    34921 aagctcgggg cgacactgcg ctccaaatcg gtgctcgtaa ccggccccgc cggggtgatg
    34981 gcctccaaat gccccactac gcgatcgaac gcctctcgac cgccggtgga cggcagcccg
    35041 cgcgtttgct cgtgggatcc gatgaaatcg agcgccaccc gatctccgcc gaggagcgcg
    35101 atgcggccga gcgccgccgc gatcagcgcc gcgtactcgt acttggatag ggaggcccat
    35161 tcactgcgat aggccatcga cgcgctggca tccacgagca ggcgcaccga gcggtcggtc
    35221 tccgtctcga actcacggat caacagtagt ccgtgacgca tgcgcgcatg gcgatcgagg
    35281 aagcgaaggt cgtcccctgc cacgtagttg cgatgcccac cgaactcgat gccggcaccg
    35341 cggcgcacgc tacggtgcgc tcccgcgtat accccgtccg ccaccgcctg cgcgcggatg
    35401 cgcagcggcg ccagcttgcc ccaatcgatc ttttctctga tcgccatagc ttggggcgtg
    35461 cctcacggat tgggcttcgg gtcttcgcag acgcgcagga ttttcgggct cacttcgagc
    35521 ggatccagct tggcttgagg atcgcgcttc ttccattcct cgcaggcggc gtgcgccagc
    35581 ccggttgctt ccacggccac gtaggcttcg agcagctgcc gatgcaccac cgagagttgg
    35641 ggctccgtga gggcaccggt ggcgatgaag cgactgccac gcgcgatggc ttcgacatag
    35701 cgcccggcgc gcacgtccga gatcaggagc ggaatctcct gcgcgatgcg gcgctgcgtc
    35761 ttgagtgatg ctccggccga ctctgaacac agattacccg catcacgcgc ggccgccttc
    35821 atgcccgctt cggtcaaagg caaattggtg agcggtacca agattacgct gccgggttcg
    35881 agcccgttct tttcgagtcg gttgtagtga gcgagcaccc acgcgcgatt catgtcgccc
    35941 atgaacttca tcgcgatgct caccagcgtg tcgcccggct tgacgagcag ccgtagattg
    36001 taggggacca cgatctcggc gccctcttcc ggcagcagcc aaggcttcga gtcgttggac
    36061 atggagatca cgtccgaacg tcgctcgtcc ccgagcagct cgagcgccag cgcgtcccaa
    36121 gtttcgccgc gtgcgatcac tcgatgtgac agcgcgggca cctccagacg catgcctcgc
    36181 acgatcgacg agccgccctc cacgtcgagc caattggcgg ccacgagcac gtgctcgaac
    36241 tggatcttgc cgtagtactt ctcggcgatc gaggccaggg tgtcgccggt ccgcaccgta
    36301 tgcgtaaagg ccagcgcggg cgtcggctgg cccaagagcg agaccaacgt ccagagcacg
    36361 caggcgatca ccgcacggcc gctattccaa aaaggcgtcg attggaggcg cgcgcgcagg
    36421 gttcgcccgc tcacggttgg gaagactctc acgatggcgg tagcctggcg ggccgtgcgc
    36481 gcagcgtcaa cttcgcgcgc acttcctccg ttgaaccgga cgcgagccac gaacttgcgg
    36541 cccgcgcgag ctcgggagtt cttcaggttt tcgcgtcttg aaagtcggcc ggcagctgat
    36601 cggctcgcgt gacgatctcg ccgatgcgtt ccgactcgat gccgcgccag aagacgctcc
    36661 agcggggcag acgcaacatg ccgtcacaga cggactggta ccaaggggtg atcgggttgt
    36721 cgttgcggct gcgccaaaat agactgcgat agtcgcggca gatggcttgc cacaagtcgt
    36781 tgccgatgcc ctcgccttgc gcttctggtt cgacggcaaa cttgctcaga taaggcgctg
    36841 gtccctcgtc gtgcaggatg gcgacgccgc gataggaagg ttccaggtaa atcgcgagcg
    36901 gcgggcgatt gaagaactcg ctcaggaggc tgcgaccgaa gctcgcttcc aacagtcgag
    36961 tcaggcgctc ccgatccagc tccgagtagc tgccgagccg ctgcacgacc gtgcccgact
    37021 tgatgagcgt gccggcgcct ttgacggtga acagctcctt gagcaggttc aacggcgacg
    37081 tgatgctaac gagcggaggc gttggatcgt tggcggcgat gaaggcgctt gcggcctcca
    37141 atagctcggc ttcgcgcttg ccgagcacgc ggctctcgag cagcgcctcc cgatccgcac
    37201 gcaggttgat caccgaaatg cgcccactgc tcgtgggcag ggcgtgaccg ggcccgagtt
    37261 cgaccgggcg atctccgact ttgctcatga gcccgccgcg gcgccgcagc agcacgatct
    37321 tcctcgtatc cagcgcaaga gagagctcgg cgagctcgcg aactcgatcg ggaaggcgca
    37381 gctcgggtgc cgggcgaaag tggagaatcg gaatccgttc gcgattcagt tcttcacgga
    37441 cgcgctcggc caagtgcggc tcgttcatgt cgtgcgggat tgcgggtaag ccgacggtcg
    37501 ggaggcgtcg cagcaggcgg tctgcggacg cctgcgcgcc ctcggttccg aaaagaccca
    37561 gcacgatcgg cgcgaacagg ccgaggtcac cgaggaaacg cagctgctcg acgagcgagc
    37621 cgagcgcgtg acgaatcacc ggaccaccgg gagagatgat ggcgaagctc tctttcggca
    37681 gctttcgaaa gagccggagg taaagctccg cttcggagcg tcgccccacg ctctcgagaa
    37741 atgtgagtac gacctcggcg gtagtcgcag gggctggtgg gggcacccta gaatcccgct
    37801 cggctaccgt aaccgaaacg gtttgggctg cttggacgca cctcacgcgc gggcgagctc
    37861 gcgtccagcg ccacgagttc gctggcgagc tgcacgagct gcggattacc ggcgcggatc
    37921 gtcacgttgg acggcaagcg gcgcacgtac cagtgcacgc gcagcgtggt tcgcccaccc
    37981 ggcgggatgg tgctgaatgg accctgattc tccacctcga cataggtggg atcggcataa
    38041 atctcgactt cgccctctcc cggagcggcc tgagccggcg acaaatccgg gaaaactttg
    38101 acgagtaaaa ggccctcgcg ggtcacgtga gcaacccagc ctttgccgtc cccgttggcc
    38161 ttggctttga cgtctcgaat cgagtgcgtg taccagaggc agcccgcgcc ctgactaaag
    38221 ctgattgcag cgcgctccgg tggcgtgaac ggcagtgagt cggccgcgta aaacgtgagc
    38281 ccaccggcgg gaactcgcgt gatttcccaa gccgcggtgc tgcgcgccac gttcgcgtgg
    38341 ttcgtgagct cgtattccgc gtggattgcc cggcgatcga gatctgctcg gaagcgctta
    38401 gtcaagctga gcccgttcag cttcggaagc gcagcatcat cgaggggcgg cccgctcagc
    38461 tcgaaaccgt tgaccagctc acgcaccgcg aaggcagcgc gatcgatacc agccggcggc
    38521 ggccagatgg gccagtcgga ttgcggactg gtccaaaaag tggagccgta gttgccagga
    38581 tcgatctcag gccccgttag gagctcgagc ccacccaggc taacccgggt aatacggcct
    38641 gctacaccag gcagcacttc gaacagcagg tcgccaaact cgaggacgca ccgatcgccg
    38701 cgcaggatag ggccgttcaa catcgcacgt cggccgctgc taccacccca ggctgaggtg
    38761 cgcaacggca aatcaagagt agggctctcg tgttcccgag cgcgcgtgct ttgttacctg
    38821 gcgcgcggtt actcgatgag ctgggcttac ctgacgcgcg cgttgaacga ggcgttgctc
    38881 aggaaccgct cggcgcgggg gcggccaccg ctcagcgcgg caagcaacgg atggtgcccg
    38941 ccgaaagctc gctcgtgacg tgatagccca cgcggctcgc gaccagaatg tcgtcaccga
    39001 gggtgacccc ctcagggctg atcaatcccc cgctcgcagc gggattgctg aattgaaggc
    39061 gattgctctt aggcgcccag gtggagccgt cgagcagcac gaactcgagg tgattgtcgg
    39121 gcatgcaccc tgcacaaacg tagatgtagc tgccctgcga ccagagcaga ccgacgtctt
    39181 ctccgcgggc gaataggaaa ggctgattat tgacgcggct cttctcccag gattggaggc
    39241 gcacgtcgtc tgccaaccgg cgaccatccg catccaggcg cgtgagcaaa acggtgctgc
    39301 ctagtttttc gccttcgtag gttccctcgg tgaaggagcg cgcgagtaga gcctcgccgc
    39361 cccgccagag gagtgcgtga ctgcctcctc cgtcactgtg ggtcgagcgc gcgagctcat
    39421 gctcgtcccc cagggttccg tccgctgcga tgcgtcgcgt cgagacgact tggaaagcgt
    39481 tcgtgccatc ccagccgttc tcgacatagc tgacgagaaa gccttggccc gtcgacgtga
    39541 tagccgcagg ccacaaccaa ttcgagttgg tcgcgactgc gttcggagag accgtcagag
    39601 ttccgtcatc cgtcaggagg ccaaacttca ggtcgtagcg tcgcccctca ccgtccgcca
    39661 attcgtccca aagaagcgca atccccgtgt ccgctcgcac ggccagcatg cggctcgctg
    39721 ctccgccatc gcgtgggacc ggcttcatag gtgccctcaa ggtcccgtca gcggacactt
    39781 gacccacgag tagctcccgc tcaccctggc gcatcgccaa caacgtcatc ccatctgcgc
    39841 ccggtacgag cagaacgttg gagaagtact cggaggtttg ttcgaccgcc gtgctcacgg
    39901 cgcccagctg cgcgtcgccg ctgatcggcg caaaacgcac gctctgtttt tgctctggtg
    39961 catcccattt gggcgcctga gcccaagcca cgaaggcgtt cgagccgagc gtcgcgagac
    40021 caccggtgaa cgtgtcgttg gttggaacat ccaatagccg cgccgggctg ctcacggagc
    40081 agtcgtacgc gagctgcgcg ggccgctcga gaatggggag cgcgcccgct ccgcccgttc
    40141 cccccgagcc cccagttccg cccgctccgc ccgttccccc cgagcctccc atcccacccg
    40201 gcgggtccgt ctcgctactg caaccgagag caccgagggt ggtgagcagc gcaccggcaa
    40261 gtaagagttt ggacaggcag ctgtgcggcg gggctaaaca tagggcgtgg cggtgcatgc
    40321 acggccccgc agcaattgcc ttgccaacgc ggctcgtgcc ctgcgggttg gctcggagtg
    40381 ccggatctcc caagatactt cgcgaactgg cctctccggc agcgtcatcg gcgccagggg
    40441 gccgatggag atggcacaac ctggtacacc gcactcgaat ccaatcgggg ctgaacgagc
    40501 cattggcaag cacttcgcct gcttcgggtg atcgaatagc cggagcttgg gtgcgcgggc
    40561 caatttcgag cacgaaactg ccgttctaac ggcttctcga gtaaagatgc cgccgatgac
    40621 tcgcgctggc tttctggctc cttccgcgct tctggccaac cccgcccgaa cttggcgccc
    40681 cttcgcgcgc aaatcgagcg ttctcttggg ggtatttctg ctcgcgtcga ccgtgaccgc
    40741 tcagcgcggc gagagccccg aggcgctgct ccggcgctat ccgttcgatc ccgcctgccc
    40801 gtttggtcgc atttcgaacg gtaaaggcat gatcgtacgc tgtatcgaag aacgcgaggc
    40861 gactgggctt ctgcagcaaa gcgcaggtca acccacgccc gcgctgtcgg cccatgcggg
    40921 atcgagccaa caaagccccg gtcaaggtcc cgcaccaggc gccagggatg aaggaacgcc
    40981 cgccctttcc agcacgaccc cgtcgacgac caatccgccc gccgaaaccg agccggtcga
    41041 ggcccccggc agcgatcgat tcgacgtcgt ggtcgggccc gttgccgcag atcagggtga
    41101 cctgccgctg ggcaagctgg ggcaggcaaa gtcgcgttac gccaagtgcc tcgcggacaa
    41161 cggcgggttg cgcgacaaat cggccgaggt gcaggtccga ttcctggtgc gttccaaggg
    41221 tattgccgag ggggtgagcg tccaaaagcg cattggcgtc agtgccgaag cggcgcgctg
    41281 cgtcgccgag gtcgtcgatc gtcggcgtgt gggcacgccc agcgcaccca tggtcggagc
    41341 ctctgtgacg atccgtttcg agaagactgg caaatgacgg acgccgcgtc gggtgcccac
    41401 gcggatcgtt atcgtgcggg ggttacccgc gcgcaggccg aggccgaacg gttgggggcg
    41461 cgctcacgtc tgctctccaa cctgagaggc ttgagtttcg gcaccgcgct gctgagcgga
    41521 atcgcgggct ttgccgccga agcgtcgagg ggcctgagct ttacgatctg tgggctcgcg
    41581 gtgctcgcct tcgcaacgtt cgtggtcctg cacggccgta tcttagaggt gctggacttg
    41641 gcggagcggc gcgtggacgt gtatcagagc gcgttgctgc gtctggccca tgcgtgggac
    41701 aagttgcccg acgtcgcaga aggtctggcg ccgaacgagc acccctattc gggggatttg
    41761 gatctattcg gatctggttc gctgtttcaa cgcctctccg tcgcgcgcac ccgcttcggc
    41821 cgtgagcgcc tagcgggctt gctgctcgaa gcggacccca atcagctaga tgaaatccag
    41881 gcgcgccaag ccgccgtgca ggagctcgcg caagcgcacg agctgcgtga agagttcgaa
    41941 gctcacgccc tcggcgtggc cggaaaaacc ggggccgctt ccggtgcgcc gcgcgttcgc
    42001 ttggctccca atccgacgca gctcttgagc tgggcagagt ccgagcccga gcttctgaat
    42061 gatcccgcga gcgtctgggg cgtgcgcatt ctgcctccgc tcacgctggc ggcgctggtg
    42121 gcctggctcg ggttcgggct gaccttcgtt cccgctctgg ggttgttgtt tctgcagggc
    42181 gtgattttga tgcgcgcgca gcgcgcggcc tcgcgcgtgt tcaacgcggt gtccgtgacc
    42241 gagggtgcct ttctccgtta ctcggagctg ctgcgcatct tggagcgggc caaattcgaa
    42301 gctccggctc tgcgcgccct cgtccagcgt ctcgatacgc cggtgggctt gccctcgagc
    42361 gccatgacgc gctttcggcg ctgggcaggc tggttcgatc tccgtcacaa cgggctcgtc
    42421 tatccagtcg tcgacctcgt cttgctgtgg gacgtgtggt gcacgctcgg tctcgagcgt
    42481 tggcagcgcg ctgcgggcaa gcacttgcgc ggttggttcg aggtgatcgg ttggttcgaa
    42541 gcctactcgt cactcgcctc tttcgcgtcg gatgagccgg gggtgagtta tcccgagctg
    42601 gcccgaaccg ggaccgagtt ccgcgccgag cagcttgctc atccgctaat cgatcccgcg
    42661 cgccgcgttg cgaacgacgt gctgctgggg accgaacagc gcgcgctgct ggtgaccggt
    42721 tcgaatatgt ccggcaagag tacgttgctg cgcgccatgg gcttgaacgc ggtgcttgcg
    42781 ttcgccgggg cgccggtaac ggcacgacgc ttgcgtctct cacccattgc ggtgcgaacg
    42841 agcatccgag ttgcggattc tctcgagcga ggcgttagcc atttttacgc agagatcgtg
    42901 cgtctcaagg gggtggtcga ttcggcttcc aacccaaccc cggtgttgtt cctgctcgac
    42961 gaaatcctgc acggcaccaa ctcacgcgag cgccaggtcg gcgcgcgttg ggtcttgacc
    43021 gagctgctgg ctcacggtgc aattggcgcc atctccacgc acgacgagga gctgtgccgg
    43081 ctgcctccgc acctgatgca gagcgtggcc ctggtgcatt tgcgagaaag cgtgcagggc
    43141 ggcgcgatgc acttcgatta caaggtttac ccagggcccg tcacgagcgg caacgcctta
    43201 cggctgatgc gcgtcgtcgg cctgcaggtg ccgttggagt gactcgcgag ggcggccttc
    43261 tgggtcgcgc atcccatcct tagggaatgg gtttcggtcc cgggttcttt agagctggcg
    43321 gttcagtacc agtatttggg gtaacccctg cgcgcgggca cggcgttgtt gaagagcagc
    43381 gcgacgagcg tgacgattac cgcacccaac agggtgggct tcacgatgaa cgcccaggac
    43441 tggtgcccca acatcacgat caacgggttc gagcccgcgg ggggatgcac cgtgcgggtg
    43501 agctgcatca gcgcgatcgc cagggccaaa gccagcgcca tgctccacca gtgataaccg
    43561 agcaaagctg cgaccacgac cccggtggtc gtcgagagca cgtgacccac gatgacgtga
    43621 cgaggctggg aaaaagggct ttccggatat ccgaacacca gaacgcacgt cgcgccgaaa
    43681 gagcccagca ccaacggggt tccggtgaca tcggtcagtg agcccaccgc ggcgatggcg
    43741 aggagcccac ccaaaaacga gaacgccacg tgcgcgagcg atggtcgcgg cggtgccccc
    43801 gagctcaaac gcggcttagc tacgaggtcg acggtagttt gcggtgtagt cacagtctga
    43861 atccctccgc acggattctt gcgtcggagc gaggaggggc acaacgcgca tttatcgggg
    43921 aagtgcagcc ttgcatccgt tcgcttacct aatcccacac tgagcgcgta ctcgatgcgt
    43981 gaccccgagc ggctgaaccg cgcgagtcga gctgctcgat ccggctctcc tatcggcacg
    44041 tctggaaagc tccttccccc aactcctggg acgcccgttc gccgactcag ggactcgacg
    44101 ctgtccgaac tcgacagagt aacgagtcag gctatcctga agagttcgga atatgttgga
    44161 attcccgagg gtttgctgct gctaccacgg tcccgatcaa tcgctgcccg gctcgcctca
    44221 ttccgtagta cgaagccggg acgtggtcgg cgaacacgcc gaactcgggt ggaccagtga
    44281 gtgagcagga cgagaaaact cgggtgacgc aggtcgagcc gggtcctccg cccgatcagg
    44341 gcaccggatc cgattgcttg gtcgtgatct acacctcgga gtccgggctg ctcggtaaac
    44401 gtttcagcct cgatcgcagc attctccggg tcgggcgcgg cacggacaat cacatcgtgc
    44461 tcgagggcga cagtgtgtca cgccgccacg cgcatctcga acgacgaggc agcgattggt
    44521 acgtggtgga cgacggatcc accaacggca cgtacttgaa cgaagaacag gtcgtgcgag
    44581 agagcgtgct cggcaatggt gaccgcatca agatcgggcc cacgatcttg aagtttctgt
    44641 cggggctcga cgccgaagcc aagtactacg aagagatcta tcggatgacg atcgtggatg
    44701 ggctcacgca gatccacaac aagcgttatc tgttcgaggc gctggagaaa gagctgattc
    44761 gtgcgcgacg ctacgagcgg gagctcagcc tcgtgatgtt cgacatcgac tacttcaagc
    44821 gcatcaacga ccagttcggc catctcgcgg gggactacgt gttacgcgag ttggctcgcg
    44881 tagttcagga gcgcatccgc cgtgacgagg tgttcgcgcg ttacggcggg gaagagttcg
    44941 tgatcgtgct gccggagacg ggcttggggg gagcgctggc tctggccgag aacttgcgca
    45001 agcgggtgaa cagccatggc ttcgtgtttc agggcgacag catcccggtc acgatcagcg
    45061 taggctgcgc gcagctctcc aaggacgatc gcaccgcgcc ggacttgatt cagcgggccg
    45121 acgaaaagct gtacgaagca aaacggggtg gcagagatcg cgtcgagggc taagaggggt
    45181 gcccgatggc ccgttcgccc ttgctcgccg agcagccgcc gcttttgcgc ggcccacttc
    45241 ccggcccccg ctcgctggag ttgctgaaat cactctccgc ggttgcggcg ccgatggggc
    45301 cacggccgcg gctcgaggcc gaaccggcgc agcccgatcc gcgtgacggt gcgacggagt
    45361 cgcgcatcgt ctacgcgtcc gcgcgcggag tgaacgtgca ggacgcggac ggcaacatct
    45421 acgtcgatct cgcggctggt ttcggttccc tgttgctcgg gcacagccac cccgcggtgc
    45481 tcgccgctct gagcgaacaa agccaacgcc tgatgcaagg tttgggcgat gtttacccgt
    45541 cggagctgaa gctggctctg ttgcagcgcc tgacgcggct ttacccgggc tctgcgcggg
    45601 catgcctggc tcagtcgggt tcggatgccg tgacgtgcgc cttgaagagc gctgctctgg
    45661 cgaccgggcg tcctcgcatc ctggcgtttc aaggcgcgta tcacggtctc ggctacgggc
    45721 cgctcgccgc ctgcggcttg cgcgagagtt accgaacgcc cttcacggag cagctcaacc
    45781 cgcacgtcac ctggctcccc tatcccgcga acgagcgaga ggcggaggcg tcgctcgatg
    45841 tcgcgcagcg agagctcgca cgctcacccg tgggagccgt gttgattgag cccgttttgg
    45901 ggcgaggcgg cgtgatcgta ccaccagcca cgttcctgcc cgacttgggt aaactcgcgc
    45961 gggaaggggg cgcgctcttg atcgccgacg aagtgtggac tggcctcggt cgatccggcc
    46021 attggctctt cagtctggat cacggcgtga tccccgatct gatctgcctg ggcaaaggcc
    46081 tcggtggagg gctccccatg agcgccgtgc tggggcgttc cgagatcatg caacattgga
    46141 gtcgggaagc cgaggtggtg cacacctcca ccttcgcagg cgcgcccctg gcatgcgcca
    46201 ctgcgatcgc gaccttgcag gtgctcgagc gcgatgagct cgtcacgcgc gcccgatgcg
    46261 tgggcgaagg ctttctctct cggcttcggg ccctctcgac gccggcagtt cgcctgagct
    46321 gtcgcggtgc cggtttgatg atcgggatcg agagcgagcg ccccacggaa ggttgggggg
    46381 ctcgcttgca aaagcgctta ctcgcgcggg gctacattac ttccaccggt ggcggcgcgc
    46441 gggacgtctt ggtgctcact ccacccttgg gcatctcgca agccccgctc gatcgattcg
    46501 tgaccgcgct cgaagccgcc ttgtcggagg tgacgacgtg agccaatcgg ccctgatcgc
    46561 ggagtccgag gccttgcacg agcgcgtgcg cgcattcgcg aagcaggcac ggggcgaagc
    46621 agatttcgaa cgcttggcgc tggagatcgc tgaataccaa gctcgctatc agcccggata
    46681 cgctcgcttg ctccgacgat gtggcaaaac accggatggc gttgcggcgc tgcccgcggt
    46741 gcctacggat gccttccgtt tgacgcgcgt cgcggctcat ccgcccgcgc tcgattctgc
    46801 atgcttcgtg acgtcgggta cgaccggtca ggaccaaggt cgacactact tccggaccac
    46861 gcgcacctac gcagaacttt cggtggagtt cgggcgccgc gcgctgcgct cctcgccggc
    46921 gcgatgcacg gccgtcgctt tggcgcccgc gccgagtgag ccaccgagct cttcgctggg
    46981 cttcatgtgc ggcctgttca tggagcaatt cgacggccgt ccgctcgtcg aagcggggca
    47041 cggggttacg gcggccttcg acgtcacggc cgccgagcgt tggttattgt caccggggca
    47101 catcgacctc gaggggcttc gccgcgcggt agcgtgcgcg cagcgccgag ccgagccgat
    47161 gttgttgctc gcgacttctt tcgcgttggt ggagacgctc gatgccctcg atcgtgcgaa
    47221 ggtcacgacg ctgccgctcc cggctggcag cgtggtgatg cagacaggtg ggtacaaggg
    47281 ccgcactcgg gagatcccgc gcgcagagct gcggacctgg gttgcgcgag tgttcggaat
    47341 cgccgaggaa caggtggcct ccgagtacgg gatgaccgag ctctccagcc agctctacga
    47401 agcgtgcgtt cccggagccg agcttgccgg tccacgcgag ctccacctcg ccccgccttg
    47461 gctcagggtc agtgccgtcg acccggtgag cttggagccc gttgccgagg gagaagtcgg
    47521 cttggctcgc ttcgtcgact tggccaacgt ggacagtgcg ctggtgattc tgacgcgcga
    47581 tgcgatccgt aagcgtgggc ccttcatcga gttgctgggg cgcgcgccgg gggctccgcc
    47641 acgtggctgc tctttggcca ttgaagcatt gctcgcgtaa ggaggcgctt gaacgcttcg
    47701 ccggataaac aggctcgcgt caggatcgat cgcctgctcg ccgcggcacg cgcgctccag
    47761 gatccgggct cctttccggg acgagatctg cgtgcgcggt tgctgggcag tacgggcctt
    47821 tcggaagcgg gcgtggagct cgggctcacg gagtacctgg agacgcaccc gagcgagacg
    47881 gagctggatg cgctctgccg cgcggtgcag ccgagcccgg tcagtcacgt gcttttgagc
    47941 gccaacgtgt tcattgcagc gcatcgagcg ctcgccttgg cgctcgcctc atcggcgacc
    48001 gtatacgtgc gcccttctcg acgggagcca gaattcctga ccgcgctcgc ggcactcgac
    48061 cccgaactat ttcacgtggt cgacacgttg gagcccaagt cgcacgaaca gctgacggct
    48121 tacggccgtg ccgcgactct ggccgagctg cgtcagcagt tgccggctgg agtacagttc
    48181 agcgcggcag gtccgggtca cggggtggcg ttcattcacg aatcggcttg gcgagcgtcg
    48241 ccgggaactc cggggcggca aacgagcgat cacgcgccag atggcacccc gatcgatcag
    48301 ctaagtcgtg agctcgtacg cgacgtggtg gcgttcgatc agcgcggatg tctcagcttg
    48361 cgctgggtcg tcgtcgagtg ctccgagccc tccatggccc ggctcgaacg gttggcggtt
    48421 ggtatctcga ccgcgctgag cgccgccgaa gccagagtgc ctctcggacg gctcacgggt
    48481 gaggagctcg gcgagcgtcg acgttacctc gacacgatga gctacgtcgg ggaggtgatc
    48541 gacaacggta ggagcagcgt gtccgtgctc tcgcccgagg ccgcgccgct gttgccaccc
    48601 gctggtcgca acgtgctcct ggtgtgtaca gcggacgccg cgtcctatgc caagcgctat
    48661 gccgattcga tcaccagcta ttgtgtgagt catgcggaac agcccgattt cgagcgcgcc
    48721 gtgcagaagt gcctcccgtt tgcccgcaag gcgactgcgg gacggatgca acagcctccg
    48781 ttcgatggcc ccgtagactt gagaccagcc actcgtcctt cgaccctgtg aatcggtcaa
    48841 tcgcttcaag tgtgcgtgag ctgtcggtcc ccacccgcgg tgctcgatgc atgacgtagg
    48901 accgcccagc cgcccacgat ttgaagcagg ccgagcgcca gaaaccccaa atcccacgcg
    48961 agctctcccg aacccggatg cacgtgatga attcccagca gctggtgatc gatgagccct
    49021 tctatgaagt tgaacaaacc ccagcccaag cacatcgcgc ccacgaacgt cgatgccgac
    49081 caaggaactt cgctacgtcg agccgcggcc cagagccgcc aaatcccgag cgcgcacatg
    49141 acccaagtga aggtgtgaaa cagtccatcc cacaccatgt tgtatttcat gctcagcagg
    49201 tccgtgggtg gtcggatgct cgagagcatg ttgtgccact gcagaatctg gtgcagcaag
    49261 atgccgtcga cgaagccacc tagaccagtg ccgagtaaca agcccgccga gacgagcggc
    49321 gaagcataat gggctcgccg ggcgctactg cccgcgtcgt gttcgccccc tactcctcgt
    49381 ccggagtttg cgcgtccgga gcttgctcca gattcgctgc gtgctgcgat caccgcgacg
    49441 gccaggaacg ggagcagggc taccgtgaga ttagcgccga aatgttcagt ccagacgctg
    49501 gcgcgagctg cgcgccccaa ctcacagttg ggacaagctc tacaaattgc agcgtccaag
    49561 cagagcgccg cccccgcgcc gagcgccaga gcgatgcgtg cagggccgct acaccgccgc
    49621 cagttttgcg cggcggcgcg ttccccgatt cgtggttgtg tgcctgaagg agggagtctt
    49681 ggccgcatgc agggagcggg gcaagatcca gacccgtgca gctcgcgcgg ccctgggacg
    49741 ctacgtgctc gaaagcgctc taagggctgc tcgcgaccag cggtaagcgt cggcgcgcaa
    49801 tgacgtgact gggggtgcct tcggcggcca gctccgcgag cggaagatgc tgctgcttgg
    49861 cgtagactgg gtgtttgccg tgctgcttgt accactcggc gaccgtggcg gtcatcttca
    49921 tcttctcgat gatttggcgg aagccgacgc cggtgttgta cgcacagaac gagatctcgc
    49981 cgagctcggt tccgtacggg atgatgcaca tctccgtacg tcggaaatcg tagttgaaca
    50041 gatcttggaa ccacataccc gcgacgaaca ggaagcgcca ttcgaattgc ttcgcgtcgc
    50101 cctcgctctc gccgatactg cggcccccgg ctccgatctg gctctggaac tgccgaacca
    50161 gttcgagcaa cccgtagccc tcgggcgcgc gctccgcgtg gaagttcctg accaaagcca
    50221 agctcatgcc gagcagggtt tgtttgcgcc ccaatcctgc atccgcgatg cgcttcatgt
    50281 cattgagcaa gttctcgatg tcgagaaact ccgccaccgg aaccatgcgc ttggtgcgtt
    50341 tgtggacgag taacaccgtg ccgatgccgc aattcgggtg gcagccgcac ttgagcgcgc
    50401 cgaattcggc actttgcccg agcagcagat cgaccacgtc gctcatcggg ttcatggcgc
    50461 tgagcggaaa ccaatcgcgg agcggctcgg tctgtccgag ttggctcttc aaatcgtggg
    50521 ccaggtggct caacgtgtag cgctgccggg ctcgatcttc gtccgagatg tcctcgtctc
    50581 gacccgtgaa gctcaccggt tggaatgaaa cgaccgtaat tttgtcggcg ttgtcgatcg
    50641 cgaatttgac gatctcgccc acctgatcgt cattcacgcc gttcaccacg gtgaccacga
    50701 gcaccacatc gatgccagcg cgggcgaggt tctcgatggc cttcagcttg acgtcgaaga
    50761 ggttgccgac cttgcggtgg ctattggcct cgttggtgac cccgtcgaac tgcaagtaac
    50821 acaagcgcaa tccggccgcc ttggcctgca tgcagaactc cggctcctgc gcgaagcgga
    50881 tgccgttgga cgcgcactgc acgcaaaagt agccaacttc ccgggcataa cgcacgctct
    50941 cgagaaagtg cgggctcaac gtgggttcac cgcccgagaa ctggacgctg agttggcggc
    51001 gcggctggat gctcaaggcg tcatccagaa tcttcttcac gccctcgaaa tcgagctcgt
    51061 gaacgtaacc cacttggttt gcatccatga agcacgggtc gcacatcatg ttgcagcggt
    51121 tcgtgaggtc gacggtgagc accgagccgc gcccgtattt gatcgacgaa gtgccgtgtt
    51181 gacggagctc actcttcggc gattcgaaat cacgccccgg gaataggctc tcgatgcggc
    51241 gcaagaatgc cgggtcgatg ctcatgacgt cttcgaagcg gccatgcttc gggcacgtct
    51301 tggtcatcca aatccggcca tctcgctcct cgatgttggc cttgatctca cccgggttcg
    51361 agtccacgaa cgcgcggtag tcctggtgac ctgcgagcac ggcgctgcgc gcttcacgca
    51421 cgcataccgg gcaaagtgag tccgtctgcc gcgggaagcc caacggcggt gaggtccgct
    51481 cgcgcgcccg tagcagaggc gccggagccc acttggggtg aagaggcgga ggggccttct
    51541 gctcggcgcg agctgcgagc caagggaaag cgagatccgc ggcacgggag agtagctttt
    51601 cggcctccac accgatctgt cgaagcatgt tcagcgggta gcatactcga gaatggcgcc
    51661 gcggctgcgc ccgtcgcgct tcggccgcct gttcagggcg gcaccctctc gcgccggtct
    51721 gcggcgtttc gagactcgag gaccttactg tgttcgtcgg ttcacgcttc ttcgactgtc
    51781 ctgtgtgcgc gaggtcacac tgcggatctt ggcgcaggag tgcctcacct cctgtgagcg
    51841 cgttcttcga ctccgcgcgc acttcggagt aagccactag agttctcggt gtccccgttc
    51901 gtcattctca gcgatctgca tctcgacccc ggcactcacg ctaccgcgga ggcctgcgcg
    51961 cgcttgatcg ccgcacatcc ggggtacgaa gtcgtgctct cgggcgacat tttcgggctg
    52021 tccgaggacc ctccgagctt gactccccaa acctccgtgc agaaaacgtt ggccgcgcat
    52081 cccgagctgg cccgagccat gcggcaacat ctgttggggg gggctcgctt gacgtggatc
    52141 gccggcaacc acgacgcggg gctcaccgta cccgggatgg cagagacggt cctgcgcagc
    52201 ttggatttac ctgaggcgcc gctgcggatc gagccgtggt tcatccgtcg gggaggtgtg
    52261 cacatcgagc atgggcacgt atgggatgcg gacaatgccc cagtgcatcc gctgggcgat
    52321 tggtcgctgc tcacggagcc gctcggggtg gcgctcactc gacgtttcgt gtcgaaacac
    52381 gcttcgcact tcgcgcacgc tcacgagacg accttggtgc aggggttgaa gcgagctttc
    52441 gggttgtatg gggcgcgcgc gccgcttttg attctgatct acttcggcgt ggccgcgggc
    52501 atttgcctgg aagccttgtt acagaccgag atggacgagg agcgtcaaca aggtgccggg
    52561 cgcagccgcg cgcttgccga ggagtgcggg atctcgttgg agagtcttca agagctgctg
    52621 tcggcgctgc ccacgcccac tcacacggag ttcggcagca ctttctttcg gctctacttc
    52681 gaccgggtgt tgttggcgtt actctgcgga ggcgcggggg tatggtcgct cgcgcggctc
    52741 agtccgatcg ggctcggggt aggcgtggtg agcgcggccg tgctgcggca caacgtcaag
    52801 cgaaccggag ctcgctatca gaacctaccg atccgacgtt tacgtcacgg cgcggcaatc
    52861 gcgaggagtc tgatgcgggc ggacgtagtc gtgtttggac acacgcacgt gccaacctgt
    52921 gaagacggct acgtcaacac cggctcgttc gcgtacttcg acaagcaact aggtaggccc
    52981 tacgtgcacg tggatgggca ggctcatttg gagctgcggc gcttcgtgtg aggagctccg
    53041 aggcctctag gacttgcggc gttcagaact ctacccgcgt cggcttggtt tcgcgcagtg
    53101 cgctcggcgt caacccgagc tgcccgcgct gattgctgcc ccaacacaga acgtcgttgt
    53161 ttcggagcag cgcgcagctg tgggcttcac cgagcgcgag ctgccgtggc gcttcgagat
    53221 ccagcgcgac ttcgagctcc gtgggctcgt tcagttgagc aagtagagcg ctcccggcct
    53281 gccccgcgtg attggcgccc caacaataca aactcgaatc gaatgagccg catacgtgcg
    53341 ccgcattggg gccggacacg agccaatccg gtgagtcaga cggcagcaat tcggccatga
    53401 ccgggagctt caccgtactg cccaggaccg cgagctggcc gaaccagttg tttccgaaca
    53461 ccaatggttg gctgcctcgc gccgtgacgg ccaacgtgaa atcgcgtccg agcgccagct
    53521 ggctgacggc ctgctcgccg acggggctcg cccgcgcgac gatgcgagtg tcctggccgg
    53581 cttggccgaa gtcgttggct ccccaacaga gaagcaggtc gttcgcggtc gtaccgcaga
    53641 tgtgccggcc cccggcggcc aagctcacga acgattccgg cgagctgacg agggtcggca
    53701 gaaatcccgc gctgccggcg gacccggtgc cgagctgtcc gaaggcattg ccaccccagc
    53761 agtaggcttg ccctttggcg tccaacgcgc aggcgaaatc actcccacac gtgaccccga
    53821 agagctcggg aaagttggct cgagccggtg gcgtcgaggt gctggagccc ggcgcagaac
    53881 tcgacgcagc cgagccggta ggggaaccgc cggcgaatat ggcgtagcgg ctggctggga
    53941 attgaacccg attctcgacc tccgttgcct tcgcgtcggg cttcacgccg ttgccgagct
    54001 gcccgctctg attggcgccc cagcacacta cgtcgtagaa accagggctg gggccaaacc
    54061 acacgtccgc gcatgtgaag tcgcgtcctg cgcagaggcc gccgccactg aagcgaatgt
    54121 tcgatcgaag gagactcgag ttcgcggggc cggaaccggg agcggcggag ttcggagcgc
    54181 cggagtttgg ggcgttggct cccgccgcgt tggggtagag ccccggagtg gagccggacg
    54241 agctcggttc actggcgaaa accacagcgc ggggctgggg ctcatctcca ttcagcgacg
    54301 aaccattggc gagttgcccg gactcgttgg cgccccagca ctcgaggata cgcggtacgt
    54361 cgatggggtc ttgctccgga tcgcgcccga gctcgccttc gaacagtacg caggtatgcg
    54421 ctgcgcccgc ggtgacgcgc acggggcgga cgcagccgaa gccttcttca cagtggctgc
    54481 cgggcaagca gaggtgtggg tgagtgctga agccttgccc cgtctcgcaa acgtacgcgc
    54541 gctttttatc ggagctgcag gtgctcgtgc cgggttcgca gctcgccgct aagcacccct
    54601 gtatgggatc gcaagccgcc gcgtcgccgc aatcgcgcag gactcgaggg gtgccgtagc
    54661 gagccatcag cttcaactcc gggccctcgc aatagcgctc tccagcagcg gcgggcaaac
    54721 accgacccag gtaagcatca caagcttcgg ggccgcagtc ctgcagtagc tgctcgcgtt
    54781 caccggttcg agagcactgc accaagcgag tcccgtcgca gcgtagcgcc cccggtacgc
    54841 agcgcctgca cccgagcgtg ccgttggcgc agagctcgcc cgtggtacac gtctctcgtt
    54901 ggcactgcgg ctgctcagag tcgcatcgtt catactcggt ggcaccggga acgcacacca
    54961 gactttccac gtagggagac gagttggatg cgccgctctc gcaatgagcc ggcgttgccc
    55021 cgtcttcgac acaggccctc gtgcaatggg cgatggtcac gtaaccggcg gcgtcgcccg
    55081 cgtagtagcc gttctcgtct gccatccggc tgttggcgag cacacaatgc tggagctcgc
    55141 ttccagagca gcgggcgatg cccgccgcac aaagctcggg aggcgggcaa gctttgaccg
    55201 cgagccccaa ctcgcaggcg accggcgagc tgcagacatc cagtagctcc cattgaccgt
    55261 tggctctgca gatcgagagt tcggtctccg tggtgccgcg acataccacg cttcccggcg
    55321 cggccttggg cgagttgcat aggtttgact tgcagacacc gagctccgcg tcacacagct
    55381 ctcggtggcc gcagtcttcg actaagttca gctgtgcgcc ggcctcgtca cacttcagca
    55441 acttgcctag ctcgcacgtc gcacggcccg gcacgcaggt gcggcagcgc ccggcgcggg
    55501 catcacataa gggggcactc gaatcgcagc gttcggcctc ttgggtccct gaacccaagg
    55561 cattgcagct ccggaggctc ggcgcgctcc cgggaaaggc ctcgcaatac atcgcgtcgc
    55621 gcgagcagat ctcgcaatat ccgagctcgc cctcggcgtg gcacgtttcc ttttctccac
    55681 accttcgcac gacttgatag ctgagtccct cgagctcgca ttgttcgagc accgctcccg
    55741 tgcagcggaa ggtgccgggc gcgcaggcca ggcagctctt gtgggcggcg ttgcagcgcc
    55801 cgaaatcgcc gcactcgact tgaggcacga accggccccc atcacacacc tcgagcgtgc
    55861 cccgctccgt gcagcgtgcc tgggcgcgcg tgcagatgtt cggcagagcg ggatgagcag
    55921 tcgcggtggt gccgccgctg ttgcgggacg tgcccgggtt accccccgac ccagacgaac
    55981 ccgatgcggt cggcgcgatc tctccaccgg atccgacgcc gcagcccggc cccgctgcgg
    56041 agagcaggat cgagagcgcc catgacgcgc ggcgatgtgc gaacggctca ctcacctgga
    56101 actcactccg aggtcatcag ctgaagccta ctcgagaagc gcagacaccc gcctatcgaa
    56161 ctcagcttcg aactcgaagc tcgagccttg ctctcgagcc ctgggcctca ttcttgagtc
    56221 tgaagctcat tcttgggtct gaaccccatt ctgggacctg aacagagtgc gacggtaaaa
    56281 ctgcagctcc gcgatagact cccgaatgtc gaacaccgca tcgtgctcgc cttgcgtcgg
    56341 cttcgcataa accccggcct cgccgtacca gagccgcgag agcaccttga tcgtggatac
    56401 gtccacgagc cgatagtgca ggtaggcagc gagcccgggc atgtattgat cgatgaagcg
    56461 ccggtcttgt ccgatgctgt tcccgcataa cagtgccggg tacgggcacc atccggcgat
    56521 acgctcgagc agttgttttt ctgctgatcg gagatccgtg cgcgatctct gcacccgttc
    56581 gagcagtccc gtgcgttcat gcatgtcccg cacgtacgga cccatcttgg ccagttctgc
    56641 cgccggctga aagatatcgc aggagaactc ctcgagtggc tcgagcgcgg cgttggtgat
    56701 caccagggcg gcctggagaa ttacgtccgt ttgcgcgtcc aagcccgtca tttcgagatc
    56761 gatccaggcc aggttgtccg ccgactgagt gcgttgaatg cgttgattgg agggcaaggt
    56821 tcttttcctt ctagttgtcg cgtcgctctt tgcgctttgg cagcacgatg ctcggctttt
    56881 ccgcgcggcg tcgatacaac aacaacgtat gaccaatcac ctgtgccacg ctcgagccgg
    56941 tacgttgctc tacgccggcc gcgatctcgt caccttcgag cgaggcgtcg gccgagagct
    57001 ttaccttgat gagctcatga gtttcgagcg cggtgtcgat ggcggatgcg actgcgtcgg
    57061 ttaggccacc ccgtccaatc tggacgatag gcttgaaatc atgcgccatc gcgcgcagaa
    57121 atcggcgttg tgctccggtc agctccaacg gcgctgccgt gcggggagcg gcggcaggct
    57181 ttttggaggt cggcgctgat ttcgaggggc tcctggtctg ggcactcttg gtctgagcgg
    57241 gcttgcgctg gccgggcttg cgctgggcgt tctgggtcgg ggtgggcctc gggcgagcgg
    57301 ccttcgcagt gcgaggcgcg cgccgagtgc ggctcgattc ggacatttcc gagccttacc
    57361 tcggagtttt cggcgtgcct aggctctcga gctcgcggcg cacaggaccc ggaaaagccc
    57421 gcgaaagtaa cgcccgcgtg cgctcaccgt cgcacgcggc gaacctgacc gcccgtgaac
    57481 cgcgcccggt tggttttggc tgctggtcgg taaccgatcg cggctctgaa tgtggcatgc
    57541 tgcgcccgtg cggatacttt acggagtcgt tggtgaaggg atgggccatg cgacgcgcag
    57601 tcgcgtggtg ctcgagcatt tggcgcaggc gggtcacgac atcaaagtcg tggtctcggg
    57661 gcgggctcac ggctttttgg tcgatcgctt gcgcaaccac ccgaacgtga gcatcgagca
    57721 gatcgagggc ctgacgctca gctacctggg taacgaactc gatcacggcg ccagtatctt
    57781 caacaatctg aggaatgcgc cgaaggccgt agcgcgcaac gtggaggtgt accgcaacgt
    57841 cgcggaggcc ggatttcggc ccgagctcgt agtgagcgat ttcgagtctt gggccgctct
    57901 gtatggcata cgccacggcc tgcccgtgat cagcatagac aacatgcaga tcatcaaccg
    57961 ctgccgtcac gaacgagcgc tgttgaaagg cgcgggcttc gatttccggc tcacgcgcct
    58021 cgcggtcaag gccaaggtcc ccggcgcgtt ccactatttg atcaccacgt tcttcacccc
    58081 cgccgttcgc aagaaataca cctcgctcgt gcccgccatt ctgcggcccg aaatccagag
    58141 tgcgcgacgc gaaccggggg agcacatcct ggtttatcaa acccaatcta cccaccacga
    58201 tctgattcaa accctgaaac gcttgccgta tcagttcttg gtctatgggt tgaggcgaga
    58261 cgcagtggac ggaaacgtgc ggctgtgtgc cttttcggaa acacagttcg tggaagatct
    58321 gcggacggcg cgcggcgtgc tcgcgggagg aggtttctcc ttgatgagcg aggccgtgtc
    58381 gctcggagtt cccatgctgt cggtgccggt ggtcggacaa ttcgaacaag agctcaacgc
    58441 gcgatatttg gcggagctcg gcttcggcga atataccccg gagttgagtc gagaagcctt
    58501 gcagcggttt ctatcgcgct tggacgagta cagcatgcgc ttgcgcgagc gccctcatta
    58561 cgacaactcc atgttgttcg gctgtctcga cgagctgatt gaccgtgtgc agcggggcga
    58621 gcgccgcgtc gtggcgctcg accatgccgt gctcggaaaa tacgaccaat agagccatgc
    58681 tgagcccccg cgtccgtctg ttcgtcgtgc tagcgacggt gttcgttacc tgcctcacgg
    58741 tgggtgacat catcggcggc aagctcatcg aaactcagct tctcggtcag agtttcatcg
    58801 tcaccgtggg catgatcccg ttccccgtga cgttcctgtt gaccgacgta ctgaacgaat
    58861 tctacggcaa gcgcgcggcc cggttcgtga ccgtggttgg cttcatgatg gccgtgctgg
    58921 cctacgcttt catctacatt gcggtcgtga tcccgatcgc gccgctcacg caggctccgg
    58981 atttcacggg cgtgcgcagt gccgcgttcg ataacgtgtt cgcgggttcg cggcgcatga
    59041 ttttggcctc catggtggcg tacatcacgg ctcagctggt ggacatcttc gtgttccacg
    59101 cgctgaagcg ggtgaccgcc aacaagctgc tttggctgcg cgccacgggc tcgaccgtcg
    59161 tctctcagtt catcgacacg gtcgtgatca actcagtggc ctgggtcgga atcctcgagg
    59221 ccgatcagat tatgcgcgtc gttttctcgg cttattcgct taagatcctg atcgcgattg
    59281 ggctcactcc gctgatttat ctggcgcaca gcgtggtgca gcgcggcttc gggatcgagc
    59341 ccgtcttgct ggacgcacaa ggcgaaccga tgctcgcgcc cgaggccgcg aaggcagagt
    59401 agattcagtc cgacccgagg cgctggccgc gactcattgg ctaggttcct gcgtgctgtg
    59461 gtggtgatgc gtgtggtccg ggtcgttgcg gatgaagtct gcagtcttga agaaagcttc
    59521 cgtcagctcc tggcgcaatt tcggatccga tacgacttcg ttcaaggcga ggctcatgca
    59581 ccgcatccag gcgtcgcgct cggcggcacc aattgggaag cgcagatgtc tccgtcgtag
    59641 tcgagggtgg ccacgctcca cggaatagag cgccgggcca ccgagccatt cggtcaaata
    59701 gcgacggagc acctctttga cgggcgtgag atccggcgga tgcaggctgc ggatctgcgc
    59761 cgcctccggc agcgtatcca tgtgctgata gaagcgatcc acgagttctg cgacggcggt
    59821 agatcctccc accgcggcga agtgcggatt gtcatgggcg gccgtcgaag cgactgacgg
    59881 ggcctgataa gcgccctgct gcggggctga ctcgatctgg ggtttgtcgg gagccacgct
    59941 cggtcgatgc agcaaggcct atgccgctag aattgtgccg atccgtgcga cgtagcttgg
    60001 tctgcaacat gacgcgggcg acgtcttgtc gtgtctccaa caaatcgcgt ctggatcgat
    60061 tcgttgttgg aggggccggc gcggcgtggt gacgggcggc gggctcgagc ggtggaggag
    60121 cagagcgcga ctgacgtcag tcgcgctctt tacgttacgg ccaaagtcga agacgttcga
    60181 cgtcgcgatg cttcatgcaa acagcgggag cgcttcgccg gccaggggga agcgtttttc
    60241 gagatcggga tcgagcttgg cgaggccgcg cagggcgcga tgaatgacgg ttggggtcag
    60301 cttcacaccg tcgctgccgg tgctcaaatt gccggggttc agcttgagtg cgagctgatc
    60361 cgccgtgctg ccaccgatca cgcggttgcc ttcgattccg gggccaagca cgagggtgct
    60421 ggtgacgggc cagtggtctt tgcccgagtt ggtgttctcg ccgttgtaac gcgggccgcg
    60481 cgcaaaatcg gaggacacga cgacgaacgt gttctgaccg agtccgaagg tgtcgagctg
    60541 atcgaggatg tacgcgaggc cgccccagag tttgtagatt tggcggactt ggtcgcggtc
    60601 gtggttggcg tgggtgtcga agccgc
//
LOCUS       DASTKU010000806         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1721102, whole genome shotgun sequence.
ACCESSION   DASTKU010000806 DASTKU010000000
VERSION     DASTKU010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721102"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..742
                     /locus_tag="VFQ61_28225"
     CDS             392..742
                     /locus_tag="VFQ61_28225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958428.1"
                     /translation="MVYVNVVTFDGTGYRVWQDGRTGEVTSAPSAVGAAVSAAHDLVD
                     LVERPSDKDCGLVADALRELRTALAHARRGRVLGRIHFESTGLGTVRPASDAPGHNAR
                     TVEERISAAIAAGR"
     gene            complement(884..957)
                     /locus_tag="VFQ61_28230"
     tRNA            complement(884..957)
                     /locus_tag="VFQ61_28230"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(921..923),aa:Met,seq:cat)
     gene            complement(1484..2314)
                     /locus_tag="VFQ61_28235"
     CDS             complement(1484..2314)
                     /locus_tag="VFQ61_28235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958429.1"
                     /translation="MAIHRSVYRLSAIPILLWACSGSTSQAPTANPPTVEATAPEAPV
                     SETKQTESAPLAPAPASEAKSAESAEKQAPAHSPLAQLMRDHFREAETIRFAVIAGKL
                     EDAVQAAAKLSDKSDAGKYPKTYRTSLERMQHAAKRIQNSADIAEVTAAAADMGTACG
                     SCHEGQHGPKANVGNAPSAGESVSSRMARHSWATARLWEGLYVPSSAAWNAGARSLQE
                     DPFPEEVLKRGGVYARSAAKDFRTFAAQSLNKDSPTERATVYAALLRTCATCHLATRH
                     "
BASE COUNT          484 a          803 c          883 g          541 t
ORIGIN      
        1 gctcaggaaa gacctcctga gcgtcgctgc gctccgaact tgaccactgc aaacttggcc
       61 ctcgtggccc acaacttcta acctcagttt cagaatttag gcacccgcgt cgctcacgct
      121 ccgctcggcc gaatcccgaa ccacgcaggt ggtcaacttc agcgcaaatg ggtggtcaag
      181 ttcgctgcaa acgggcacct ggcattgtgc cgcatgagcc ggaggcgcct cgaactctgc
      241 gcggggagcg gaggcgccca gcgctggcaa gcgtcggcgc tgcagacact cgacgcaaag
      301 cgcgtcgttc acgagcgcta tctggacagg acaacgggcg caactattgc tcacgacctg
      361 ggatgcgttg ctccgacgcg agcagataac cgtggtttac gtaaacgtcg tgacgttcga
      421 tgggacgggc tatcgggtct ggcaggacgg tcgaaccggg gaggtcactt ccgcgcctag
      481 cgcggtgggc gcagccgtct ctgcggccca cgacctagtc gacctcgtgg agcgcccttc
      541 agacaaggac tgtggcctgg tggcagacgc gctgcgagag ctccgcaccg ccttggctca
      601 cgcacgcagg ggccgtgtgc tcgggaggat ccactttgag tcgaccggtc tcgggaccgt
      661 gagaccggcg agcgacgcgc ccggccacaa cgctagaacg gtcgaagaac gcatcagtgc
      721 ggcaatcgca gctggacggt gacagttcgc cctcactcac cgagatgcga aacccccgag
      781 ccgttcggcg accgccaggc gtacgtcgct cgggggcagc tttttccgct aaaattggcc
      841 cttaatgagc aatccagcga aaatcttcgt tgtccagaat tagttgcgcg gggaggattt
      901 gaacctccga ccttcgggtt atgagcccga cgagctacca ggctgctcca ccgcgcgtca
      961 gaaggtgcgc gaacatatct ctttggttgg cccagtcaag ccggaaagtt tgaaggcctc
     1021 acttttctga atccgaaagt ccaagtgcgt tcgcgtcagc cgcgaggtcc tgatctagcc
     1081 ggcgcgtgcg cgattcgacc gtccggacca atcggaggtt cggtgggcaa tgcgacgggg
     1141 ctttcatccg tggatgacgt ggcgcggttg ccgcgagagc gagcgttgtt gtgggcggcg
     1201 agcgcggttt tgagacgagc ggcgagcgct cgggctgcgc gtgtgtgggc gcacgacgag
     1261 cttcgcgccc gttgcatcga ggccgcccaa atgatcaatc cgagaagatg agtgtgagtc
     1321 gatggcgcca ctcaataggc tcgcgggctc aatgggcttg cggctcaatg ggcttgcggc
     1381 ttggtgtgaa cgggcgcgaa gctcgtcggg agttcgctct agcgcagtcg aaatgcatgc
     1441 ggcgagtcgg cttcaccacg caagccgcat gtggttgttt gcgttagtgc cgcgtcgcca
     1501 aatggcaggt ggcgcaggtc cggagtaggg ccgcgtagac ggtggcccgc tctgtcggtg
     1561 agtccttgtt cagagactga gcggcgaagg ttctaaagtc ttttgcggca ctacgagcgt
     1621 agacaccgcc acgcttgaga acctcttcgg ggaatgggtc ttcttgcaag cttctggcgc
     1681 cagcgttcca cgcggcgctg gacgggacgt agagcccttc ccaaagtcgg gcggtggccc
     1741 atgaatgacg tgccatgcgg ctgcttacgg attcgcctgc gctcggtgcg ttgccgacgt
     1801 tcgctttggg gccgtgttgc ccttcgtggc agctgccgca cgcggtcccc atgtcggccg
     1861 cggccgcggt cacttcggca atatctgcgc tgttctggat gcgcttggcg gcgtgttgca
     1921 tgcgctccaa agaggtacgg taggttttcg ggtacttgcc ggcatcgctc ttgtcggaga
     1981 gtttcgcggc agcctgaacg gcatcttcga gcttgccagc aatcacggca aaccggatgg
     2041 tctccgcttc gcgaaaatgg tctcgcatga gttgggcgag cggcgagtgc gcgggagctt
     2101 gcttctcggc ggattctgca gattttgctt cgctggcggg cgctggtgcc aaaggcgcgc
     2161 tctcggtctg cttggtctcg gacaccggcg cttcgggagc agttgcttcg acggttggtg
     2221 gattggccgt cggtgcctga gaggtcgagc cgctacatgc ccacagcaaa atcggaattg
     2281 ccgacaatcg ataaacactt cgatgaatcg ccacaggacg actcctttcg cgtgtgaagg
     2341 tgcttcgcta catcgcagct cgacacggca acctgcatgc cgcccgaact cacgtgattc
     2401 actcgagata tcgaaggctc acacagcaca tttggcgtct acccgcgggg gctgcgcagg
     2461 ccttgcgtgt ccaacatccc ggaaggtcgc cgtccgaaca catacaatcc aatgatcggc
     2521 tgttctcgaa gtcttgtccg agcggagtgg atgcccctac tgccttcgtc ccgccccggg
     2581 aaggctgcac tcgagtcgcg tggaatccgc gttgactcga actcagcggg gctgacgctc
     2641 ggtaactcag gtcctaacgc agcgcagatg agatggacaa gaacgtctcg cgctgcacta
     2701 atgtgtcggt t
//
LOCUS       DASTKU010000807         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_827619, whole genome shotgun sequence.
ACCESSION   DASTKU010000807 DASTKU010000000
VERSION     DASTKU010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827619"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..1004)
                     /locus_tag="VFQ61_28240"
     CDS             complement(246..1004)
                     /locus_tag="VFQ61_28240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9958430.1"
                     /translation="MAGSPSLAAQFYDLDKPEAPPEVLEFYRRAAQRSRGPILEPMCG
                     SGRYLLPLQAEGFDIDGIDSSPAMLDACRRQARKLKLEPRLFHQDITALQLPRRYGLA
                     FIPAGSFCLLIDPQRVRAALRELRHALLPGGELLIEIERAVDRRPTISSAWEGRWLEL
                     EDGSKIILSYLTRYSGVEQTEYSLRRYEHVHAGQLLRQEYEDFAVRFWEPESFRAHLQ
                     EMGFSQIEAFKPYTEEALQDDDEAILFRAVRARD"
     gene            complement(1141..2811)
                     /locus_tag="VFQ61_28245"
     CDS             complement(1141..2811)
                     /locus_tag="VFQ61_28245"
                     /inference="COORDINATES: protein motif:HMM:NF003007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HET9958431.1"
                     /translation="MTWLVQLFTSNTIAHAILSLALAAAVGLALGRAKIRGIGLGIAG
                     TLFSGILLGHFGLHIDEHILEFAREFGLVLFVYTVGMQVGPGFFDSLKRQGFAMNAMA
                     GTIVLLGVSLAVASHFFLNIPVPAAIGILSGATTNTPSLGAAQQTLELSPNLTQELGH
                     MPAIGYAVAYPFGIMGIIAVMLLVRVLFRIRPDTEAEAYELQLNERSKELDSRSVLVE
                     NHNLVGRSIGEIPSYRESGVVITRRMRGTDLQIARDDMTIEAGDILQLVGKAETLDNL
                     VTIIGRVADTDLRSVSEHIVARTILVTNSEILGHTIGSLMLRDRFGVTVARVTRGEFK
                     FTGMLNMRLMLADKLFVVGPEAGIEEAAKLLGNSLKALDHPQALAVFIGIACGVLLGS
                     VPISIPGTPVPLKLGIAGGPLIMSVILARLRRVGPLNFYMSASANLMVRELGIVLFLT
                     CVGLKAGGSFFHVLLHGPGLTWMAIGAGVTFIPLAVVAVIARRAFKMNYMPLCGLLAG
                     SMTDPPALAFANTLAASDGPSVAYATVYPLTMLLRVVAAQLVAIVLFN"
BASE COUNT          645 a          932 c         1040 g          567 t
ORIGIN      
        1 cttcatgggt tatcgatacg ggatcttcaa ctacgcgggc tcggccttgg gcggatacgt
       61 gcgagtgaaa tcattcacgt tggcgggccc tgcctccaac tagagcgcag ctcataacct
      121 gcgcaagtgg cttgacgaac cttctccaga gcgtttggaa gacgagtttg tttcatggcg
      181 gctcgccaaa gccttggcca aaacctggct ttgcggcggg cgttctgaac tgtctgacag
      241 tagtgctagt cccgagcgcg cacggcacga aacagaatgg cctcgtcgtc gtcctggagt
      301 gcttcttcgg tgtagggttt gaaggcttcg atctgcgaga agcccatctc ttgtagatga
      361 gcgcggaagc tctcgggctc ccagaagcgc accgcgaagt cttcgtactc ttgccggagt
      421 agctgccccg cgtggacgtg ttcgtagcgg cgcaggctat attccgtttg ctcgacgccg
      481 ctgtaacgcg tgaggtagct caggatgatt ttcgatccgt cttcgagctc gagccaacgg
      541 ccttcccagg cgctgctgat cgtgggtcgc cgatcgaccg cgcgctcgat ttcaatcaag
      601 agttcgccgc ccggcaacag tgcgtgccga agctcacgga gcgctgcgcg aactcgttgt
      661 ggatcgatca acaaacagaa cgatccggca gggatgaacg ccagcccgta gcgtcgcggc
      721 aattgcagcg ccgtgatgtc ctgatggaaa agccggggct cgagcttcag ttttcgagct
      781 tgtcggcggc aggcgtcgag catcgcgggg gacgaatcga tgccgtcgat gtcgaagccc
      841 tcggcctgca agggcaacag gtagcggccg gagccacaca tgggctctag aatcggtccg
      901 cgcgaacgct gggcggctcg acgatagaac tcgagcacct ccgggggggc ttcgggcttg
      961 tcgaggtcgt agaactgcgc tgcgagcgaa ggggatccgg ccatgctcgc tcatacactg
     1021 cgtcgcgctg ggcggggagc cgtgagcgat cgcaggcagc atagagctcg gatcgagcgc
     1081 acaaaaaggc cgcgcagcgc gacggaggtc gtagccgtcg cgctgcgcgt gaggagagcg
     1141 tcagttgaag agtacgatgg cgactagctg cgccgccacc acccgcaaca acatcgtcag
     1201 ggggtacacg gtggcatagg cgacagaagg accgtcggag gctgccaagg tgttggcgaa
     1261 tgcgagtgcg ggcggatccg tcatcgagcc agcgagcaac ccacagagag gcatgtagtt
     1321 catcttgaag gcgcgccgtg cgatcacggc caccacggcc agcgggatga acgtgacacc
     1381 cgctccgatt gccatccagg tgaggccggg gccatgcagc agcacgtgga agaacgagcc
     1441 acccgctttt agccccacgc aggtcaggaa caagacgatc ccgagctcac gcaccatcaa
     1501 gttggcgctc gcggacatgt agaagttgag cggacccacc cggcgcagcc gcgccaagat
     1561 gacggacatg atcagggggc cgccggcgat gcccagcttc aagggcacgg gggtgccggg
     1621 gatgctgatc ggcaccgagc cgagcaacac gccgcaggcg atgccgatga agaccgcgag
     1681 cgcttgcggg tggtccaggg ctttcagtga attgcccaac agcttggccg cttcctcgat
     1741 gcccgcctcg ggaccgacca cgaacagttt gtcggcaagc atgaggcgca tgttgagcat
     1801 gccggtgaac ttgaattcac cgcgcgtgac gcgcgccacc gtgactccga agcgatcgcg
     1861 caacatcagc gagccgatcg tgtgcccgag gatttcggag ttggtgacga ggatcgtgcg
     1921 cgccacgatg tgctcggaaa cactccgtag atccgtgtcg gcaacccggc cgatgatcgt
     1981 gaccaagttg tcgagcgtct cggctttacc gaccagctgc aggatgtcgc cggcctcgat
     2041 cgtcatatca tcgcgggcga tctgcagatc cgtgccgcgc atgcgccgcg tgatcactac
     2101 gccggattcg cggtaactcg ggatctctcc gatcgagcgc cccaccaggt tgtggttttc
     2161 gaccagcacg ctgcggctat ccagctcttt ggagcgctca ttgagctgca actcgtaggc
     2221 ttcagcttca gtgtcggggc ggatgcggaa cagcacgcgc accagcagca tgaccgcgat
     2281 gatgcccatg atgccgaagg ggtacgccac cgcgtagcca atcgccggca tgtgaccgag
     2341 ctcttgcgtc agattgggtg acagctccaa cgtttgttgg gctgcgccga gtgaaggtgt
     2401 gttggtggtg gctccggaca gaatgccaat cgcggcgggc accgggatgt tcaggaagaa
     2461 gtgggatgcg accgccaggg atacgccgag tagcacgatg gttcccgcca tcgcgttcat
     2521 ggcaaaacct tgacgcttca gggaatcgaa gaagccaggc ccgacctgca tgcccaccgt
     2581 gtacacgaac agcaccaagc cgaactcgcg ggcgaattcg aggatgtgct cgtcgatgtg
     2641 cagcccgaaa tggcctagca gaattccgga aaacaaagtc ccggcgatcc cgaggccgat
     2701 gccgcggatc ttggctcgtc cgagggccag gccgacggct gcggccagcg cgagcgacaa
     2761 gatcgcatgg gcgatcgtat tggacgtaaa gagctgtacc aaccaagtca tgacagagct
     2821 tcacctgccg ctcgaaaccg aacgctgcgt gtcgagacag gaggcttcga cacgttagcg
     2881 aatcagatcg tcgcggctgt ttccgttttg gcacacccac tcgcgtgcac gtgggggcat
     2941 cgcgaggggg cgaatcgact tatcaaggtt taagaaagct tcggcggctc cgtcgagtcg
     3001 cgccagaatg agcggttttc accggcgggc gagcgatgtg gctcgaatgc cggcaaatcg
     3061 caagtctgtc gaagcaaagc aggcgacctg ctcccgagtt cagcaacgtg ggtgccagca
     3121 gtgcgtgttt accgatgctg gatcagcgat ccagcatgaa ctgactgcta tacggagcga
     3181 atcc
//
LOCUS       DASTKU010000808         6614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_827805, whole genome shotgun sequence.
ACCESSION   DASTKU010000808 DASTKU010000000
VERSION     DASTKU010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827805"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFQ61_28250"
     CDS             complement(<1..479)
                     /locus_tag="VFQ61_28250"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET9958432.1"
                     /translation="MKVAIVTLFPELFEAFLKTSFVARAIAGQSLEVHLEPLRVHGLG
                     RHLSVDDTPYGGGSGMVMRPDCVLAGIEAAERALGVERADRRLLLSPQGRRFDHTAAR
                     EYSTVASMVLVCGRYEGFDERVRYYVDGEVSIGDFVLTGGEIPAMCVLEACVRFLPGV
                     "
     gene            complement(505..1002)
                     /gene="rimM"
                     /locus_tag="VFQ61_28255"
     CDS             complement(505..1002)
                     /gene="rimM"
                     /locus_tag="VFQ61_28255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET9958433.1"
                     /translation="MNRLEVARISRPHGVRGVVRVEPHWAGSDALEAHERVELVLESG
                     ERREFLIENATRTGKSYLVKFRDVNDREGADTLRGARLFVDREELAPNEMYLVDLVGA
                     EVVGPDGFVGRVISVEVNPSLDTIVIERPDGTRINQALLPVYVARLDAQARRIELSSL
                     DGLFE"
     gene            complement(999..1235)
                     /locus_tag="VFQ61_28260"
     CDS             complement(999..1235)
                     /locus_tag="VFQ61_28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET9958434.1"
                     /translation="MSLKDLISTIAQALVDDPDGVEVTEIDGDHSSILELRVAKGDIG
                     KVIGKDGRTAQSMRTLLSAAAIKLGRRVQLDIVD"
     gene            complement(1710..1955)
                     /gene="rpsP"
                     /locus_tag="VFQ61_28265"
     CDS             complement(1710..1955)
                     /gene="rpsP"
                     /locus_tag="VFQ61_28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET9958435.1"
                     /translation="MAVHIRLSRHGTKKSAFYRIVVTDHRSPRDGRFIENIGVFDPAG
                     KFEIDRARLDYWRGKGATPSDTLDRLLKKNPTPAATA"
     gene            2233..2508
                     /locus_tag="VFQ61_28270"
     CDS             2233..2508
                     /locus_tag="VFQ61_28270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET9958436.1"
                     /translation="MSSPREALLKVVREVLAPLISADQGELYLVSVTDSSVQLHLGGR
                     YAGCPGNTLTRRRIIEPVVQNAVPHAEVVVTSGALVPAGAERIVSQD"
     gene            complement(2702..3544)
                     /locus_tag="VFQ61_28275"
     CDS             complement(2702..3544)
                     /locus_tag="VFQ61_28275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958437.1"
                     /translation="MKFSRFVTVSAIAAGLTLISASAFAGDKAQGKGAQAASSAGNIK
                     ESFQISPKGLKWGMSLEAIAKLYDKVIEDEMLPLFQKAQPGLELDAVSEEQKNRKGEL
                     RRSRIEFGNTPTGVDQSPLKGEYSYRNSESLAVITLPSGTKRHFFFFNDRLWKVYDEH
                     KLKKGGGLGENFQEALKALTKRFGVPPKVVPADYDKGRPFDEAEWRDPDKVIRAVDRG
                     QVLAVVYADRKIQENLANYRKNKVELGGEGIDKDVAFVTKSAEEPKPSDKGKDKDKNA
                     KPKK"
     gene            3928..4776
                     /gene="map"
                     /locus_tag="VFQ61_28280"
     CDS             3928..4776
                     /gene="map"
                     /locus_tag="VFQ61_28280"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET9958438.1"
                     /translation="MALDMYLRSSSIEIKTPKEIEAMRTVGRLAADTLTRVGAMIRPG
                     ITTESINDFVHQDTLAKGARPAPLNYRGGGSVPFPKSVCTSVNEVVCHGIPGTYVLKE
                     GDIVNVDVTHIFQGFHGDTSATFYVGRKLSPEAKLVTEVARRCLDLGIEQVRPGGRIG
                     DIGAAITEFAHAQGCAVVRDFVGHGIGRKFHDEPKVAHYGQWGKGARLKPGMTFTIEP
                     MINVGSWEIEVLDDDWTAVTADGTLSAQFEHTVLVTETGVEVLTARSSALESSEIFPD
                     FFETRS"
     gene            5381..>6614
                     /locus_tag="VFQ61_28285"
     CDS             5381..>6614
                     /locus_tag="VFQ61_28285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958439.1"
                     /translation="MRAKSRRRVEKPKKRKPKARAVKGKRELQREKARLAREKEREKA
                     RSLREKEKDARDKEKERAAKEKEKEKEKERLLKQKEQERLAKEKEKQEARDAAVKARE
                     EARIQRQKDREAERIARVAQREAEREEARRVKEAERARRDAEREAYRKAKEAERERLR
                     AEKEAARRALEGKVARASKRAQRADGLGRGGGTTRVYRPDAIPDQSRTTRRAGDPSPV
                     QLVGIRPIVITPPQAPTPAPTAPVAAVTAPSAAASGASGAVDEATGSVADGAATSVEG
                     SGVEPGSVASSQAQPGSGAAPNSAAQPVSVAQPSSVSAPLSTAQAGSSSGADAGGRPT
                     PAPPPLPAQSVEERWARINERLQNTDAAFQREYRESFDMSWIYHDSALEGVVYTFQEL
                     KTAVDPSVPVVSDSSLQPV"
BASE COUNT         1335 a         2079 c         2089 g         1111 t
ORIGIN      
        1 acgccgggca gaaaacgcac gcacgcctcg agcacgcaca tggcggggat ctcacctccg
       61 gtgagcacga aatccccgat cgagacttca ccatccacgt agtaacgtac gcgctcgtcg
      121 aacccctcgt agcgcccaca aactaggacc atcgaggcga cagtcgaata ctcacgagcc
      181 gcggtatggt cgaagcggcg cccctgtggg ctgagcaaca gtcggcgatc ggcgcgctcc
      241 acgcccaatg ctcgttccgc agcctcgatc ccggcgagca cgcagtccgg ccgcatcacc
      301 atgccggatc ccccgccgta gggcgtgtcg tccacgctca agtgtcggcc caacccgtga
      361 acgcgcaacg gctccaggtg cacctcgagg ctctgcccgg cgatcgcgcg cgccacgaag
      421 ctggtcttga ggaaagcttc gaaaagctcc gggaacagcg tcacgatcgc gaccttcatc
      481 agcgactgcc gtacttccga cgtctcactc gaacaagccg tcgagcgaac tcagctcaat
      541 ccggcgcgcc tgcgcgtcga ggcgggccac gtacacgggc agcaaagcct ggttgatacg
      601 agtcccgtcc gggcgctcga tgacgatggt gtccaagctc gggttcacct ctacggaaat
      661 gacgcgcccc acgaacccgt ctggtccaac gacctcggcc ccaacgagat cgaccaggta
      721 catctcattg ggcgccagct cctcgcgatc cacgaacagg cgggcaccgc gcaacgtatc
      781 cgccccttca cggtcgttca cgtcgcggaa cttcaccagg taactcttgc cggtgcgggt
      841 cgcgttctcg atcaggaact ctcgacgctc gccgctctcc aacacgagct cgacacgttc
      901 gtgggcctcg agcgcgtcgc tgcccgccca atgcggctcg acgcgcacta ctccgcgcac
      961 cccatggggg cggctgatgc gagcgacttc gagccggttc aatcgacgat atcgagttga
     1021 acgcgccgcc cgagcttgat cgcggcagcg gacaacaagg tgcgcatcga ctgggcagtt
     1081 cggccgtctt taccgattac cttgccgatg tctcccttgg ccacgcgaag ttcgagaatc
     1141 gagctgtgat ctccgtcgat ctcggtcacc tcgacaccat cgggatcatc cacgagagct
     1201 tgagcaatcg tcgatatcag gtctttcaag gacatttgta ttccaaaagg gacgagccga
     1261 gtgcgcgatg cgccagcgcg gcgttagctg ctggggagca ttgtagccga gccaaccgcc
     1321 gagcgaagcc tcgaatgaag gcgggacgcc agaacgccgt ggcccgacgg accaagaacg
     1381 ccgcggagca cgactgaccg atcgacattc gcagagccac caacagcttc aggctagttc
     1441 agcagccgtt ggcgatcgtg aaagcgctcg ccgtttgcgg gtcggcctca tctgaaattc
     1501 gcgagtctga ttggcatacg acctcccgtc catctgcccc gaggcggatc tctgcgggaa
     1561 accgagccag acgcgcctgc acctgggcac gaacggagcg ccctgcggcg agtggcgcgc
     1621 tcttcagtgc aaaaggcgct gcgctgtcgc actaagcgaa tgcgctgtcg agcggtgccg
     1681 ccgtggacac cgctcgaact ccgaaaaact caggccgtag cagccggagt gggattcttc
     1741 ttgagcagac gatccagcgt gtccgatggg gtcgcaccct tccctcgcca gtagtcgagg
     1801 cgcgcgcgat cgatctcgaa cttccccgcc ggatcgaaca cgccgatgtt ctcgatgaac
     1861 cgcccatcac gggggctgcg gtgatccgta acgacgatgc ggtaaaacgc ggacttcttg
     1921 gtgccgtggc gggaaagacg gatatgaacg gccattgtgg ttcgagttcc tcagagttcc
     1981 aattcgtcag acttgcctgc gtcgggctcc gaacgagccc ctaggccagg ccggcgcgtg
     2041 gcggagaggg gtagcacccc aacgccgcgg cgccagtcaa gagctctgac gccggtacgc
     2101 ggcctccgga cattcagcct caacgggcta ccagccggga agcccatcta cgggacccga
     2161 gggaactccg tcaagaggga atctgacgta aaaaggttcg gaggcgcgac gcgcgtgtta
     2221 gagtccccag cagtgtcgtc gccgcgagag gctctactga aggtggttcg ggaagtattg
     2281 gcacccctga tttcggccga ccagggcgag ctttacctcg tctcagttac cgatagcagc
     2341 gttcagctgc atctgggggg gcgctatgca ggctgcccag ggaacaccct aacgcgccgc
     2401 agaataatcg aacccgtggt gcagaacgcg gtgcctcacg cggaggttgt cgtgacgagc
     2461 ggggccttgg tgcccgccgg cgccgaacgg atcgtctccc aggactgacg ccggtagttt
     2521 ctcgtgcttc gcgggcagag tccgcggtgg gatcagagag ttgcgacccg ccgcgggcgt
     2581 cgactgaagc gcggctcctc aacggcctga atcccggaaa cctcgtcccc cgaacgcaac
     2641 agcgccgaga ggagcgctga acgctccgcc cggcgccggg aatcgggaac gctgaaccga
     2701 actacttctt gggtttcgcg ttcttgtctt tgtctttgcc cttgtcgctc ggcttcggtt
     2761 cttcggcgct cttggttacg aacgccacat ccttgtcgat gccctcgccg cccaattcca
     2821 ccttgttctt acggtagttg gcgaggttct cctggatttt ccggtcggca tagaccaccg
     2881 ccagaacctg accgcgatcg accgcgcgaa tgaccttatc gggatcacgc cactctgctt
     2941 cgtcgaatgg acggcccttg tcgtaatccg cgggaacgac cttcggagga accccgaagc
     3001 gcttcgtgag cgccttcaga gcttcctgga agttctcccc cagaccgcca cccttcttca
     3061 gtttgtgctc gtcgtacacc ttccagagcc ggtcgttgaa gaagaagaag tgccgcttgg
     3121 ttccgctcgg caaggtaatg accgcaaggc tctcgctgtt acggtagctg tactcgcctt
     3181 tgagtggaga ttggtcgacg cccgtcgggg tattgccgaa ctcgatgcgg cttcggcgca
     3241 actccccctt acggttcttc tgttcctcgc tgacggcgtc gagctcgagg ccgggctggg
     3301 ccttctgaaa cagcggcagc atctcgtctt cgatcacctt gtcgtaaagc ttggcgatcg
     3361 cttccaagga catgccccac ttcaagccct tgggcgaaat ctggaaactt tccttaatgt
     3421 taccggcgga actcgccgct tgagcgccct taccctgggc cttgtccccg gcaaaagcag
     3481 atgcggatat cagagtcaga ccggcggcga tggcggaaac ggtaacgaac cgggaaaact
     3541 tcatcgaaac tcctccaaga gaagccagtg tgggagcggg cgcggaaaat acacctcgtt
     3601 gccttcaggc tcaagcaacg ttgctgcatt ggacggatgc agtgcctgga ataatcacgc
     3661 acacgagcga gccggttgac agccctactc cagctcgtgc gattcggacg ccaagcaggg
     3721 tgcgggcgcc cctgcggcgc agcttgatcg gcctagccgc ggatcgagcc gcgggcggca
     3781 tagggctagc cggtaggagg accttgcgct agatggcctg gacccgcaga ctcgaggagt
     3841 ctcgctggcg cagtctccgt ccgggaatcg cgtgcggcgc gcggcgggcg tctccaagag
     3901 agggctttcc tctcctcatg aggatgagtg gctttggaca tgtatctacg cagcagctcg
     3961 atcgagatca aaacgcccaa ggagattgag gccatgcgca cggtggggcg cctggcggcg
     4021 gacaccttga cccgcgttgg tgccatgatt cgcccgggca tcacgacgga atccatcaac
     4081 gatttcgtcc atcaagacac gctcgccaag ggggcgcgac ccgccccgct caactaccgc
     4141 ggaggaggta gcgtgccttt cccgaagagt gtctgcacct ccgtgaacga ggtggtgtgc
     4201 cacggcatac cgggtactta cgtgttgaag gagggtgaca tcgtgaatgt ggacgtcacc
     4261 catatattcc agggcttcca cggggatacc tccgctactt tctacgtcgg tcgcaagctg
     4321 agcccagaag caaaactcgt caccgaggta gcgcgaaggt gcttggattt ggggatcgag
     4381 caggtgcggc caggcggccg catcggtgat attggagccg caatcaccga attcgcgcac
     4441 gcgcaaggtt gcgcggtggt gcgtgacttc gtcgggcacg gcatcggacg caaattccac
     4501 gatgaaccaa aggtcgcgca ctacggccag tggggaaagg gcgcgcgcct gaagcctggg
     4561 atgacgttca cgatcgagcc gatgatcaac gtaggatcat gggaaattga agttttggac
     4621 gatgactgga ccgcagtgac ggcggacggc accttgtccg cacagttcga gcacacggtg
     4681 ctcgttaccg aaaccggggt cgaagtgctg actgcgcgca gcagtgcgtt ggagagcagc
     4741 gaaatattcc cggatttctt cgaaacgcgg agctgaagct agccgagcca ctcgtcgcgt
     4801 gcgcgcctcg ttcgaaaggg tttttgcgag cgcacgcggc tctgaagtgg ctcgcagcgc
     4861 agcgcagcga tccgtcttcg cgctgagcgc cgcactcgcg cacacaaatc acgctcgaga
     4921 ctacgccgtc agcgcacagt caacttgacg gagtgcgtgg cgggcgacta cgttgcgccg
     4981 gggcgttgcc ccagggacgg ctgtcccggc gcggctgtca agcccaaaag gatcggggct
     5041 tttgtacagc ccgacggagt ctgtgcggtc gattcggcca gcatggcagt gcagcctgag
     5101 gatttaggcc gagtcgctgt agccttcgat gtccgctcgc aagaagcaca agcaacccac
     5161 gagaacgtcc cgccgaaaat cggctcgagg cgcgcgcaaa accacacgcc ggcgcaagcc
     5221 agtcagccga ccacggaagg cacgttctgc aaaatcaggg acccgttcgc agaaagcgaa
     5281 ggggcgcaag tccgccgcac gcggacgctc ggcgaaatcc ggcagccgcg ctaggtcgag
     5341 ggaaactcga gcaagcgctc gcgcgcggga aaaagctcgt gtgcgcgcca agagccgccg
     5401 ccgcgtcgag aagcccaaaa agcgaaagcc caaggcccga gcggtcaagg gcaagcgtga
     5461 gctccagcgc gagaaggcgc gcctagctag ggagaaggag cgcgagaaag cccgctcatt
     5521 acgcgagaag gagaaagacg ctcgcgataa ggagaaggag cgcgccgcaa aagagaagga
     5581 aaaggaaaaa gagaaagagc gccttctcaa gcagaaagaa caagagcgcc tagccaaaga
     5641 aaaagaaaag caggaggccc gggacgcggc ggtgaaggcg cgggaagaag cgcgcatcca
     5701 gcgtcagaag gaccgcgaag ccgagcgaat cgcgcgcgtg gcacaacgcg aagcggagcg
     5761 tgaagaggct cgccgcgtca aagaagcgga gcgcgcacgg cgtgacgccg agcgggaggc
     5821 ttatcgcaag gccaaagagg ccgaacgcga gcggttgagg gcagaaaaag aagccgcgcg
     5881 gcgtgctctc gagggcaagg tcgcacgggc gagtaagcgc gctcagcgtg cagatggcct
     5941 gggacgtggc ggcggaacga cgcgcgtcta ccgacccgac gctattcccg atcagtcgcg
     6001 gacgacccgt cgagcgggtg atccttctcc ggttcaactc gtcggcattc gaccgatcgt
     6061 gattactccg cctcaagccc caacacccgc gccgactgca ccggttgcgg cggtgacggc
     6121 accatctgcc gcggctagcg gcgcctcggg tgcggtggac gaagcgacgg gctctgtcgc
     6181 ggacggtgcc gcaacttcgg tcgagggctc cggtgtcgaa ccaggctcgg ttgcgagttc
     6241 tcaagctcaa cctggctccg gagcggcgcc gaactccgct gcgcagccag tttccgttgc
     6301 ccagcccagc tctgtcagtg ctcccttgag cacggctcaa gcgggctctt catcgggcgc
     6361 ggatgctggt ggtcgaccta cgcctgcccc tcccccactg ccggcccaaa gcgtggaaga
     6421 gcgttgggcg cgcatcaatg agcgactcca aaacacggac gcggcattcc agcgcgaata
     6481 ccgggagagc ttcgatatgt cgtggattta ccacgacagc gcgctcgagg gcgtcgtcta
     6541 caccttccaa gaactgaaga cggcagtcga tccttcggtg cccgtcgtgt cggactccag
     6601 cctgcagccc gtct
//
LOCUS       DASTKU010000809         3273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1051697, whole genome shotgun sequence.
ACCESSION   DASTKU010000809 DASTKU010000000
VERSION     DASTKU010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051697"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1674
                     /locus_tag="VFQ61_28290"
     CDS             124..1674
                     /locus_tag="VFQ61_28290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET9958440.1"
                     /translation="MKQMETGRFMMLQASIRSALALTVLGAAVSGCTLAPRYERPSAP
                     VDSEFSTAAPNVGGAGGSGGTPLSGEASAAGSISAAASANASDAAVEARLASDIGWRE
                     VFPDPQLQDLIGRALRNNRDLRMAALNVEAARAQYRIQRADLVPNIEVAGESNSQRIP
                     SSISPTGNTYVSRSYAVTAGVTAFELDLFGRVRSLRNTALETYLGLEETRLSTQIALV
                     AEVANAWLTLVADQELLRLSRETLESQQRSLELTRMRFQSGVASEVDVHQAEIALHEA
                     EVSIATYTRQIVQDRNALTLLVGEPLPVNVGTGDRSINSQVFAQDLPAGLPAQLLERR
                     PDIRAAEHALKAANANIGAARAAFFPRITLTGSYGDADRELSSLFEGGETTWSFIPRI
                     TVPIFSGGANAANLDLAHVRKRIEVARYEQTIQVAFREVSDALVARSTLDEQLRAQES
                     LTEAASNSYRLAEMRYRNGVDSYLTTLVAQRDLFRAQQALINTRLARASNLVLLYKAL
                     GGGWTDGS"
     gene            1903..2900
                     /locus_tag="VFQ61_28295"
     CDS             join(1903..2365,2365..2900)
                     /locus_tag="VFQ61_28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558169.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET9958441.1"
                     /translation="MGTRRDLKALEQRRLRGARLLKRGYTQAEVARMCEVSRQAVSEW
                     AQALEAGGKAALRNKRLGRPPSLETDQRAELVQLLKAGALAQGFATELWTLKRVGELI
                     ARRFGVRYSHTQVWRLLGKLGFSVQRPSNKALERDEAAIAAWKKKRWPTLKKNAAKNG
                     RVIVFVDESGLSERPTRVRTWAPKGQTPVLQYHFNWHQLSAIAGVTFYRFYFRLFPGT
                     IKSPQIIEFLRALHQTVGKKLLIIWDGLQAHRSRLVREFVETTRGAIQLERLPAYAPE
                     LNPVEYVWGYLKHHALANFCAHDFAQLSDVARRKLRGMQRRPTLVTAFWKQAELAW"
BASE COUNT          638 a          974 c         1063 g          598 t
ORIGIN      
        1 cgcgatcttc ttcgtgccgt tgttcttcgt cctcgtgctg cggttcttca aggtgcggcc
       61 aagaaggatt ccgcaggata ccgagagcgg accggcgctg ccggcggcgg aggcgagccg
      121 gtcatgaagc agatggaaac aggtcgtttc atgatgctgc aggcgtcaat tcgaagtgcg
      181 ctcgccctga cggtgctggg tgctgccgtc agcgggtgca cgctggcccc gcgttatgaa
      241 cggccgtcgg ccccggtgga ttccgagttc agcacggccg cacccaatgt gggaggcgcg
      301 ggcgggtcgg gcggcactcc cttgtcaggc gaggcatccg ctgcgggaag tatttctgcc
      361 gccgcgagtg cgaatgcctc cgatgcggcg gtggaagcgc ggctcgcatc cgatatcggt
      421 tggcgagagg ttttccccga tccgcagttg caggacctca tcggtcgcgc actccggaac
      481 aaccgggacc tgcgcatggc agcgctgaac gtggaggccg cgcgtgcgca gtaccgcatt
      541 cagcgggcgg acctcgtgcc gaacattgag gtggcgggcg agagcaactc gcagcgcatt
      601 ccgtcgagca tttcgccgac cggtaacacc tacgtttctc gcagctatgc ggtgactgcg
      661 ggcgttaccg cgttcgagct cgacttgttc gggcgtgtac gcagcctgcg aaacaccgcg
      721 ctcgagacgt atctcggcct cgaggaaacg cggctcagca cgcagattgc gctcgtcgcc
      781 gaggtggcaa atgcgtggct gaccctcgtt gccgaccagg aactgctcag gctttcgcgg
      841 gagacgctgg agagccagca gcggtcgctc gagctcacgc gcatgcgctt tcagtccggc
      901 gtggcgagtg aagtggacgt gcatcaggcg gaaatcgctc tgcacgaagc ggaggtgagc
      961 atcgccacct acacgcgcca gatcgtgcag gatcggaacg cgctcacgct gctcgtgggc
     1021 gagccgctgc ccgtgaatgt cggcacgggc gaccgttcca tcaacagcca ggtgttcgcg
     1081 caggatttgc cggccggtct tcccgctcag ctgctcgagc gtcggcccga cattcgagcg
     1141 gccgagcacg cgctgaaggc ggcgaacgca aacatcggcg ccgcgcgcgc cgcgttcttc
     1201 ccacggatca ccctgacggg aagttacggg gacgccgacc gcgagctctc gagcctgttc
     1261 gagggaggcg agacgacgtg gagtttcatt ccgcgcatca ccgtcccgat tttctcgggt
     1321 ggcgcgaatg cggcgaacct ggaccttgcg cacgtgcgca agcgcatcga ggtcgcacgc
     1381 tacgagcaga cgatccaggt cgcgttccgc gaagtgtccg atgcgctggt cgcgcgcagc
     1441 acgctggatg agcagcttcg cgcacaggaa tcgctgacgg aagcggcgag caacagctat
     1501 cgactcgcgg agatgcggta ccggaatggc gtcgacagct acctcacgac gctggtggcg
     1561 cagcgagacc tgttcagggc gcagcaggcg ctcatcaata cacggctggc gcgtgcgtcg
     1621 aatctggtgc tgctttacaa ggcgttgggc ggggggtgga cggatgggag ctgaggggga
     1681 cggtttccca gattctcgga atggccaagc ctcatgccgt cccgcccttg cccagaagga
     1741 agctgtcgtg aacagattcc cgcggctcga tatccttccg gccgcgcagc gtacgttgtg
     1801 ggtgcagctc acggacatta ttgagattca cctaaatggg tgacactttt ccaattccta
     1861 cttgtcaaag cggttggtac agtcaccagc gcggagacca agatgggaac cagacgagac
     1921 ctcaaggccc tggagcaaag gcggctgcgt ggcgcgcgct tgctcaagcg cggatatacg
     1981 caagccgagg tggcacgcat gtgcgaggtg tcacgccaag cggtcagcga gtgggcgcag
     2041 gcattggagg ccggaggcaa agcggcactg cgcaacaagc ggcttggacg gcccccatca
     2101 ttggagacgg accaacgtgc agagctggta cagctgctca aggcaggcgc gctcgcgcaa
     2161 ggttttgcca ccgagctgtg gacgctgaag cgagtgggcg agttgatcgc acggcgcttt
     2221 ggtgtgcgat acagccatac acaggtgtgg cgactgctcg gcaaactggg gttcagcgta
     2281 cagcgaccga gcaacaaggc gctggagcgc gacgaagcgg ccattgccgc ttggaagaag
     2341 aagcgctggc cgactttaaa aaaaacgccg caaaaaacgg ccgcgtaatc gtctttgtgg
     2401 acgagtcggg gctctccgag cgtccaaccc gagtccgtac ctgggcgccg aaaggccaaa
     2461 cacccgtcct tcaataccac ttcaattggc atcagctctc ggcgatcgct ggggtcacct
     2521 tctatcgttt ctactttcgg ctcttccccg gcacgatcaa aagcccgcag atcatcgagt
     2581 tcttgcgggc gttgcaccaa accgttggca agaagctgct catcatctgg gatggactcc
     2641 aagctcatcg cagccgtctg gtgcgggagt ttgtcgagac aacgcgcgga gcgatccagc
     2701 tcgagcggtt gcccgcctac gcacccgaac tgaatccggt ggagtacgtc tggggctact
     2761 tgaaacacca tgcgctggcg aacttctgcg cccatgactt cgctcaactc agcgatgttg
     2821 cccggcgcaa actgcgcggc atgcagcgac gccccacgct ggtaacggcg ttttggaaac
     2881 aggcggaatt ggcgtggtaa cccgtcaaca atttacgtga aactcaataa gatcagatca
     2941 ctggatctcg ggaggatctt gcagcctcgg gatcgaaggc accttcgggt gagctcggcg
     3001 aaggggaaaa gaatcgccgc cgcagataca gcgcaacgtg cacgagaaca atcagggccg
     3061 gagtgagcga gggcctttca accaccgccc ccttttttaa acctgtcaag agacactcgt
     3121 aacaattttt cgcccgaaca ttcccgcatg cttgcttcac ttgaactcga tagcgcgcgc
     3181 attgtctcct tagacgatcc tagtgtcctg tcccggagat aactttgctt agccgttcga
     3241 gcttggcgag gattgaatcg gaggttgcgg tcc
//
LOCUS       DASTKU010000810        14751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_828787, whole genome shotgun sequence.
ACCESSION   DASTKU010000810 DASTKU010000000
VERSION     DASTKU010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14751
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828787"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFQ61_28300"
     CDS             complement(<1..394)
                     /locus_tag="VFQ61_28300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958442.1"
                     /translation="MVSESSSRRRRVRGFWLSGFILAGSAGACATDLSRDLDGFACDD
                     TGQCAPGYICNTKTNSCVRLGASGGSSGMSGSTGGTTSNVTGGRSSSGGGTGTMSSGG
                     RSSGGKPSTGSEAGTGSISEGGAQAGGKP"
     gene            complement(388..1581)
                     /locus_tag="VFQ61_28305"
     CDS             complement(388..1581)
                     /locus_tag="VFQ61_28305"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9958443.1"
                     /translation="MLRAAGCLLCFGLLLPNICWAQTTRGSRERDKAPDTLSPAPSAS
                     ADASSLGDKQKEAEKLYNKGVRAYSRGHYAQAIEAFRAADALVPSAPLSFNTALAYEK
                     LSDSGQALRCYRDYLRRAPDAENSARVKARVRQLESVLANRGVQQFTILSSPAGASVV
                     IDGVARGVTPFDGELPPGKHQVKLSLAGYQSVQAPLELRDHSVDAEYELQPEIKSPAQ
                     AEASTTPSSEASARQADSRKRESIKRETKPPTSKLQPWPWVALGAGGASLLVAGAFEL
                     SRRSAESEARDAHTQLEYADALDRVDSRQRAARIFLGVGGALVLTSGALWLIDANASS
                     SASQASGGRAVGNSNSVANSIAGDGEGRPIAARGKPTNRVVTNQIAQLGFDGQQIMLR
                     YGGQW"
     gene            complement(1585..3309)
                     /locus_tag="VFQ61_28310"
     CDS             complement(1585..3309)
                     /locus_tag="VFQ61_28310"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9958444.1"
                     /translation="MPAAPASSATLRSASPRVLPSGTIIGQKYRIDQVLVEGGMGVVY
                     KGTHIGLDKPVAIKVVRAEYAQQQEAVARFLNEARAAAQLRGIHVAHVLDTGRTDAGG
                     PYLIMEYLQGCDLRALLYENKRFDPVVAVHFVLQACEAVAEAHAAGIVHRDLKPENLF
                     LARMPDGSELIKVIDFGISKRLDARERSYTVAGQTLGSPLYMAPEQMRHPECVDPSAD
                     IWSLGVVLYELLAGQTPFEGETLPAVCLRVCGEQPAPLGKHGVTVPEGLEQAIMRCLN
                     KDPSQRYQDVHQLAEALLPFSGTTQDSAIRRIDRLLSPPHSAHPNSLHPHHNTGAPAG
                     AFPRSEALRSGAGLRSGAGLRSGTPLAGMPVSGRVVGSPAHGSTGIRASFDSDIRATA
                     FKPRFPKGKAAVVLLGAVVFFSAQFWPDLWPEGGRWLKANLGQSISGASVLAGSNVTA
                     PTLPTPRSEQRAAGEDATASSKAAEGSAPAPVINLDTASEAPSPRIAKRAQRTVWGAE
                     RRPATSPRAAQPQTLIPPPRWNAAAQLESLAAKPLPPAPVGNMEPLDLIDPYATKARS
                     TGTSKTSR"
     gene            complement(3846..4580)
                     /gene="ubiG"
                     /locus_tag="VFQ61_28315"
     CDS             complement(3846..4580)
                     /gene="ubiG"
                     /locus_tag="VFQ61_28315"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: protein motif:HMM:TIGR01983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HET9958445.1"
                     /translation="MTTRIDNAFYDTLGERWYGASDDPVALLRAESRLRNPWLCALLK
                     QRHGANARVLDIACGAGFLSNALAEGNFDVTGVDASPESLNVARDHDVTGTARYEVAD
                     ALALPYADASFDAACAMDFLEHVSEPARAIREAARVLRPGGTFVFHTFNRNWLAHLVV
                     IKGVEWFVKNTPDHMHVIELFIKPEECKAMCEASGLRVLELRGSRPVLSKAFFRMLMT
                     REVPPDFSFRFTSSTRIAYTGYAEKL"
     gene            complement(4577..5014)
                     /locus_tag="VFQ61_28320"
     CDS             complement(4577..5014)
                     /locus_tag="VFQ61_28320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958446.1"
                     /translation="MTLLALGLLALQGTIMLVDEFHFHRARGLPRWERIGHPLDTLSV
                     LACYAVALALPATRPALIGYAALAAFSCLFITKDEFVHARECQPAEHFLHALLFVLHP
                     LVLGSAAWLWYAGNRALLWGQAGLTLGFLGYQVAYWNFSRATR"
     gene            complement(5011..6207)
                     /locus_tag="VFQ61_28325"
     CDS             complement(5011..6207)
                     /locus_tag="VFQ61_28325"
                     /inference="COORDINATES: protein motif:HMM:NF014815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET9958447.1"
                     /translation="MSAPSITRFRRVRPEFQLTQGFTLEWLAQAHTRAERTRHARLGQ
                     EFDAAQFADRISRHLQRFGCGEDKIRYRYSAISDCEHTRFDEMQLYRVHENPSGAGML
                     ERSRTYDRIALSALQKLYPEIDRESAPCDLVHVTCTGYAAPSAAQRWVSESGFGQLTR
                     VTHAYHMGCYAALPALRIASGLLSGRAENSSSKLPSRRPSAHIVHTELCTLHMNPLLH
                     DPEQLVVQSLFGDGFIGYEVVGASDLAAGEAGLQVLSVDEWIVPDSAQSMTWICADNG
                     MHMTLARDVPQRLSAPLRAFVDHLLNEAGLDKSERHRAILAIHPGGPRILDLVRDTLE
                     RDESAIAESRRVLAENGNMSSATLPHIWMRLLEDPAVGEDRAIVTLAFGPGLTACGAV
                     LRKHSA"
     gene            complement(6325..7368)
                     /locus_tag="VFQ61_28330"
     CDS             complement(6325..7368)
                     /locus_tag="VFQ61_28330"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9958448.1"
                     /translation="MVEVREPSGASTRNRKSTSIGLEPISVGALGRGSQLRSGQKRWT
                     TILSAASLVTCLTGPTQAESKSPTNEPASAKTQLASRASPTSGGAGTLRVVTYNVAGL
                     PEGWSNVHPIVNLPLIAGHLAAYDLALIQEDYAYPELLRKNLALPYASAPFVRGDKLH
                     FGDGLSTFAKLPFAEPSRTAWTSCHGVVDSYFDCLTPKGFSRTTLVLGDSASKAVVHV
                     YNVHLDAGWSAADRSARARQLAQLSEAIRRESSGQALLVGGDFNLTASERDELAALES
                     RLGLRDVCTALRCSEPRRIDRFLVRSSSALELTPTRWSIDARFRDSNGRALSDHLAVA
                     IELRWRAVLPGRL"
     gene            7530..7901
                     /locus_tag="VFQ61_28335"
     CDS             7530..7901
                     /locus_tag="VFQ61_28335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958449.1"
                     /translation="MTTRHQNDAHRSTPLGPATTKNQHAKEAKPRTEQRREAAYRGEA
                     NEEASKSPHAGQPDGRNYRDRDGNAGGSHAAASPPTGKTKLPSSHANGASDSRADSQG
                     HESLSQESDARNRAARKDLRK"
     gene            7898..8803
                     /locus_tag="VFQ61_28340"
     CDS             7898..8803
                     /locus_tag="VFQ61_28340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9958450.1"
                     /translation="MSTAKNIDSQSKPLDPREAGPKPPFPEQEQDETGSERRMRPAPD
                     YGANYRGYARLKERVALITGGDSGIGRAVAFAFAREGAHVAINYLEHEEDARDTEGLI
                     RQAEARSLAIQGDIGEDSTCKRIIDEVVREFGHIDILVNNAAKQGKSVEKFEELSAER
                     VEQTFRTNIISMFNLVRHALPHMRPGAVIINTASIQAYHPSAEILDYATTKGAIVTFT
                     KGLAQSLIERGIRVNCVAPGPVWTPLIAQSFDAEKVAHFGETSPTGRPAQPAELAPAY
                     VFLASDESRYVTGEVLGVTGGKPLG"
     gene            9199..13005
                     /locus_tag="VFQ61_28345"
     CDS             9199..13005
                     /locus_tag="VFQ61_28345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9958451.1"
                     /translation="MRAVDWAKTPLGPVESWPRSLKTCVRIVLTSRQPMFVWWGPELI
                     NLYNDAYKTIVGGKHPQALGQPASVVWREIWDQVSPRAEKSMRGNEGTYDEALLLIME
                     RNGYPEETYYTFSYSPVPGDDGQTGGLICANTDDTRKIIGERQLALLRDVAARTADAR
                     TITEVCGRAAEAMATNPRDLVFVLIYLPDSQVSGEVLTLSGASGIEPGHPAAPLRLRA
                     ELARGTLAEAAPYPVAELLKSSGARSLELSEQKLGPLPCGAWDRPPARAAGLRIPLSS
                     GRSGLLIVGLNPFRLFDSSYQGFLELIASQLAAGIGVTQAYEDERRRAESLAELDRAK
                     TAFFSNVSHEFRTPLTLILGPIEDALTNAPALLTGENLRAVHRNALRLLKLVNALLDF
                     SRIEAGRARAEFRLTNLAAFTADVASAFRSAIERAGLQFQTAFSKFDDEVYVDHDMWE
                     KIVLNLLSNALKFTFDGQIRLSLQPRGQFAELTVEDTGTGIPADALPDLFKRFQRVQG
                     ARARTHEGSGIGLALVHELIRLHGGTVGVESELGRGTRFRVLIPFGSNHLPSEQVSFT
                     PASANLSNAPPFVQEAYRWLALPTDSPDSLTPTQLSRDTNRSTPPPALPLPREAPRIL
                     IVDDNADMREYLARLLSDRFMVETATDGAAALVAIRRSPPALILSDVMMPNLDGFGLL
                     RILRADPALARTPVILLSARAGDEASVEGLTMGADDYLVKPFSARELLARVETHLQLA
                     SLRNSAERERAKLYAVFMQAPVPVAVLMGAELRYEVANAHYCEVVGRNVLNQTLRDSF
                     PELGDHPIFERLEGVYKTGEAVHDPELRVALYRRGLQEDAYFNYVAQPLRDLDGRVAG
                     VIIVASDVTDQVLSRQRVNELRSTAESASRAKDEFLSTLSHELRTPLSAIIGWSSMLV
                     SGNVRPEQRQKALETIERNARAQARLIEDMLDLSRIEQGKLVLSVGPVEVIRVAEAAI
                     EAVRPAAEAKGIRLQPVLDSHATIIGDADRLQQVVWNLLSNAIKFTQKGGRVQLRVRK
                     DRSHVEITAADDGQGIEAAFLPYVFDRFRQADPSFNRRAGGLGLGLAIVRSLVELHGG
                     TVSAHSDGHGRGATFVVRLPMAPLRADRVSPVPEQSPTEQRKSFECPPALKGLKALVV
                     DDEPDTRALLCFLLEQCETSVTAVGGALEALAELQRANYDVLISDIGMPELDGYELIR
                     RVRKLPVDSNGRIPAIALTAYARAEDRTQALKSGFNMHLAKPIEPGELLVVVATVTNG
                     YQTLES"
     gene            complement(13061..13924)
                     /locus_tag="VFQ61_28350"
     CDS             complement(13061..13924)
                     /locus_tag="VFQ61_28350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958452.1"
                     /translation="MPASKWNLGFAKAGLLGAMVLFGSTQAWAGVPECGGLRLEDVRR
                     CEVRGSVSCQGGCSELGVYKKACATKLHKVCRSECTLAAEPSCSDECTVKCKQECDRG
                     VPVTCIHNCFTECAGNCSDRCAGHPDPEQCVATCEATCDGECDMQCGALVDASCYNHC
                     IECCGGACTAQANMDCQTTCQDEQFESCEYELKADCSASCNAQGALFCDGEYVLSASD
                     IVPCATALSERGIAKVNVEGSVTVNPSTAAAAARGGCAMKPPARDGSPLGLLGLALGL
                     FLASRFNKRRS"
     gene            complement(14242..14526)
                     /locus_tag="VFQ61_28355"
     CDS             complement(14242..14526)
                     /locus_tag="VFQ61_28355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958453.1"
                     /translation="MRLINENSRWALQVYRLVCVGRDVYRPRFSTIVGQAELLVFLKK
                     ADSSGSLYRTTSRVMKRLKTVDCQFISENGGWIPRPLPARPVPKRPFTGR"
BASE COUNT         2770 a         4593 c         4646 g         2742 t
ORIGIN      
        1 tcggcttccc cccggcttgg gcgcctccct ccgagatcga acccgtgccc gcttccgagc
       61 cggtggaagg cttgccaccc gaggaacgtc caccgctgct catggtgccg gtgccacccc
      121 cggaggaaga gcgacccccg gtgacgttgg aggtcgtacc tccagtagaa ccgctcatac
      181 cggaagagcc gcccgaggcc ccgaggcgaa cacaggagtt cgtcttggta ttgcatatgt
      241 agccgggcgc gcattggccc gtgtcgtcac acgcaaaacc gtctaggtca cggctcaaat
      301 ccgtggcgca cgcgccggcg ctgcccgcca gaatgaaccc tgaaagccag aatccccgga
      361 cccgacgccg cctggaactg ctctcgctca ccattggcca ccgtacctca gcatgatttg
      421 ctgaccatcg aatccgagct gcgcaatctg gttcgtcacc actcgattgg tcggcttgcc
      481 tcgcgccgca atgggcctcc cttcgccatc tccagcgatg ctgttcgcca ctgagttgct
      541 gtttccgacc gctctgccgc ctgaagcttg actcgcgctg ctcgaagcat tggcgtcgat
      601 taaccacaac gcgccggagg tcaacaccag ggctccgcca actcccaaga agattcgcgc
      661 cgccctctgc cgcgagtcga cacgatccag agcgtctgcg tactcgagct gagtgtgagc
      721 atctcgcgcc tcggactctg cggagcgcct agagagctcg aatgcaccgg cgaccagcaa
      781 ggaagcgcca ccggctccga gcgccaccca gggccaaggt tggagtttgc tcgttggagg
      841 cttggtttcg cgtttgatgc tttcgcgttt gcgcgaatct gcctgtcttg cgctagcctc
      901 ggaagatggc gtggtcgagg cttcggcctg tgcaggggac ttgatttcgg gttgcagctc
      961 gtactccgca tcgaccgagt gatcccgcaa ttccagcggt gcttgcaccg attgatagcc
     1021 cgccaacgag agcttcacct gatgtttgcc gggtggcagc tcaccatcga atggggtcac
     1081 gccgcgtgcg acgccatcga ttaccacgct cgcgcccgcc gggctcgaaa gaatcgtgaa
     1141 ctgctgaacc ccgcgattgg cgagcacgct ttcgagttgc cgcacccgtg cctttactcg
     1201 agcggagttc tcggcatccg gcgcgcgtcg caggtagtcg cgatagcagc gcaacgcttg
     1261 tccggagtcg gatagtttct cgtaagctag agcggtgttg aaggataaag gggcgctcgg
     1321 gaccagcgcg tccgccgctc ggaaggcctc gatggcttgt gcgtagtgtc cacgggagta
     1381 cgcacggaca cctttgttgt atagcttctc ggcttccttc tgcttgtctc ccaagctgct
     1441 tgcgtccgcg ctcgcgctgg gagcgggcga gagcgtgtct ggtgctttgt cgcgttcacg
     1501 ggaaccgcgc gtggtttgag cccaacagat attcggcaaa agtaagccaa aacagagcaa
     1561 gcagccggcg gcgcgcagca tgaactacct cgaggtcttc gatgttcccg tcgatcgcgc
     1621 tttggttgcg taaggatcga tcaagtccaa tggttccata tttccgaccg gagcgggggg
     1681 cagcggcttc gcggccaagg actccaattg agcagcggcg ttccagcgag ggggtgggat
     1741 caaggtctgc ggctgggccg ctcgcggact cgttgcgggt cttcgctcgg cgccccaaac
     1801 ggtgcgttgg gcgcgcttcg cgatgcgagg tgaaggcgct tcgctagcgg tatcgaggtt
     1861 gatcaccgga gccggggcgg agccttcggc tgctttgctc gacgcggtag cgtcctcgcc
     1921 ggcagcccgc tgctcggagc ggggcgtcgg cagcgtgggg gccgtgacgt tcgagccggc
     1981 cagaacactc gccccagata tggattgtcc aagattcgct ttgagccaac gcccgccctc
     2041 tggccacaag tccggccaga attgcgcgct gaaaaatacc acggcgccga gcaacaccac
     2101 tgccgccttg cctttgggga aacgcggctt gaatgcagtg gcgcggatat ccgagtcgaa
     2161 agaggcgcga attcccgtag agccatgcgc tgggctcccc actacgcgcc cggataccgg
     2221 catgccggcc agcggcgtgc ccgatcgaag ccctgctccc gatctcaacc ctgctcctga
     2281 cctcagcgct tcactgcggg ggaaagcgcc ggcgggagcg cccgtgttgt gatgcgggtg
     2341 aagcgagttg ggatgtgccg aatgcggggg gctcaacaga cgatcgatcc tacggatcgc
     2401 gctgtcttga gtcgtaccgc tgaagggcaa caaggcctct gcaagctgat gaacgtcctg
     2461 ataacgctgg cttggatcct tattcaagca acgcatgatc gcttgctcga gcccctccgg
     2521 tacggtaact ccgtgtttgc cgagcggcgc cggctgttcg ccgcaaacac gcaggcatac
     2581 cgcgggcagg gtctcacctt cgaacggcgt ttggcccgcc aacaattcat acaacactac
     2641 gccgagagac cagatatccg ccgatggatc gacacactcg gggtgacgca tctgctcggg
     2701 cgccatatac aacggtgaac caagcgtctg ccccgcgact gtataagaac gctcccgggc
     2761 atccagacgc ttggaaatgc cgaagtcgat gaccttgatg agctcacttc cgtccggcat
     2821 gcgcgccaag aacagattct cgggcttgag atctctgtgc acgatgccgg ccgcgtgcgc
     2881 ttccgccacg gcctcgcagg cttgaagtac gaagtggaca gcaacgaccg ggtcaaaacg
     2941 cttgttctcg tagagcaggg cgcgcagatc gcagccctgc aaatattcca tgatcaaata
     3001 gggcccgcca gcgtccgtac gcccggtgtc gagtacgtgg gcaacgtgaa tcccccgcaa
     3061 ctgcgcggcg gcacgcgcct cgttcagaaa acgggcgacc gcctcttgtt gctgcgcgta
     3121 ctcggcgcgg accaccttga ttgccaccgg tttgtccaaa ccgatgtggg tgcctttgta
     3181 cacgacaccc attccgccct cgaccagcac ctgatcgata cggtacttct gtccaatgat
     3241 agtgccgcta ggcaggacgc gcggtgaggc gctccgcaaa gtggctgagg acgctggggc
     3301 ggcaggcacg cgccagggaa ctgcaaccga cgtaccacta gcgtgcttgt cccaacctcc
     3361 gcagccgctg gcggcggatc gttgcctctt ttggcctccg gcgctccgac tcggagagga
     3421 ctccggtcag ctcgctctgt tatggagttt gtgacaacgc ctttggataa gcgtgtgatg
     3481 cccagcgtgg cgtaagcgta gaggcacgtt ggtgagacga ggctacaatg ccgccttcgg
     3541 tgttcggaga cgtcgtgccg tggcgttctt ctcgcctcct cgtttggacc agtttcgccc
     3601 acgtttcttg ggcgacgcca agagcggcgg tctcgctcac gagcgagcgc cggcgccgcg
     3661 accgaagaac gcgattacgt cgccactctg cagcagattg ggtgatctcc cccagcgcag
     3721 cgagcgtgtc tttcgccaag cgttccggcc ctcggtgctg gccaccaatc gcgagcgctt
     3781 gaaccaaagg gtgaggggac gggtgtgcac tcgcccggtg agcggctggg gcaaacgtgc
     3841 tttcgctaca gcttctcggc gtaacccgta tacgcgatgc gcgtggagct cgtgaatcgg
     3901 aagctaaaat ctggcggaac ctcacgcgtc atgagcatgc gaaagaaggc cttggagagc
     3961 accggcctcg agccacgcag ctccaagacc ctgagcccac tcgcttcgca catcgccttg
     4021 cattcctcgg gtttgatgaa gagctcgatc acgtgcatgt gatcgggcgt gttcttcacg
     4081 aaccactcga cgcctttgat caccaccaaa tgcgccagcc aattgcggtt gaaagtatgg
     4141 aatacgaagg tgccccccgg ccgcaacacg cgggctgcct cgcggatggc gcgtgcgggt
     4201 tcggaaacat gctccagaaa gtccatggcg cacgcagcgt cgaacgaagc atccgcatat
     4261 ggaagggcca gcgcgtcggc cacctcgtag cgcgctgttc cggtcacgtc atggtctcga
     4321 gcgacgttga ggctttcggg ggaggcatcc actcccgtca catcgaagtt gccttccgcc
     4381 agagcgttgc tgaggaagcc agccccacaa gcgatgtcga gtacgcgcgc gttcgcgcca
     4441 tggcgctgct tcagcaatgc gcagagccac gggtttcgta agcgagattc tgcacgtaac
     4501 aacgcaaccg ggtcgtcgct cgctccgtac cagcgctccc ccaacgtgtc gtaaaaggca
     4561 ttgtcgattc gagtggtcat cgcgttgctc tcgagaaatt ccaataagcc acttggtagc
     4621 ccaggaagcc cagcgtcaag ccggcttgac cccacagaag cgcgcggttg cctgcatacc
     4681 agagccaggc cgcggaaccc aataccagcg ggtgcagcac gaataacagc gcatgtaaga
     4741 agtgctccgc gggttggcac tcgcgcgcat gtacgaattc gtctttggta atgaataggc
     4801 acgagaaggc ggccaatgcc gcatatccaa tcagcgcagg gcgcgttgcg ggcagcgcca
     4861 gcgcgacggc ataacaggcc agcacgctga gtgtatccaa cgggtgcccg attctttccc
     4921 agcgcggcag gccgcgagcg cggtggaagt gaaactcgtc caccagcatg atggtgcctt
     4981 gtaaggcgag caggcccagc gccagaaggg tcatgccgag tgtttgcgga gcaccgcccc
     5041 gcaagccgtc aagccggggc caaaggccag cgtgacaatg gcccgatctt cacccactgc
     5101 gggatcctcc aacagccgca tccagatgtg aggcaaggtc gcggacgaca tgtttccgtt
     5161 ctccgccagc acgcgtcggc tttcggcgat ggccgactcg tctcgctcga gagtatcccg
     5221 cactaaatcc agaatgcgcg ggccgcccgg atgaatggcg aggatcgcgc ggtggcgctc
     5281 ggacttatcc aggccggcct cgttcaacag gtgatccacg aaagctcgca gcggcgcgct
     5341 caagcgttga gggacgtcgc gcgcgagcgt catgtgcata ccgttgtcgg cgcaaatcca
     5401 ggtcatgctt tgggcggagt ccggcacgat ccactcgtcc acggagagca cctgcaggcc
     5461 cgcctcgccc gcggcaagat cactcgcgcc caccacctca tagccaatga agccgtctcc
     5521 gaacaggctc tggactacca gctgctcggg gtcgtgcagc agggggttca tatgcagagt
     5581 acacagctcc gtgtgcacga tgtgtgcgct cggtctccgg ctcggcaact tggaggatga
     5641 gttttcggca cgacccgaga gcaggccgga ggcgatgcgc agcgccggaa gcgcggcgta
     5701 gcaacccatg tgataggcgt gggtgacccg cgtcagttgc ccaaaaccgc tctcgctaac
     5761 ccagcgctgc gcggcgctcg gcgctgcgta accggtgcat gtgacgtgca ccaggtcgca
     5821 cggggcgctc tcgcggtcaa tctcgggata gagcttttgc agcgccgaaa gcgcgatgcg
     5881 atcgtacgtg cgcgagcgct cgagcatgcc cgccccgcta gggttctcgt ggactcgata
     5941 gagctgcatc tcgtcgaaac gcgtgtgctc acaatctgag atggccgagt atcgataccg
     6001 aattttgtct tcaccgcagc cgaagcgctg gaggtgtcgc gatatgcgat ctgcgaattg
     6061 cgcggcgtcg aactcttgcc cgagtcgagc gtgacgggtg cgctccgcgc gagtatgcgc
     6121 ctgcgctagc cactcgagcg tgaagccttg ggttaactgg aactccgggc gaacgcggcg
     6181 gaagcgagtg atggagggcg cagacaaagt agcagcagct agagcaaact ccgggcctgc
     6241 acccgttact tggcacgacg ggcgtaatcc gggagtgtct aagcttcggc ctacgtcggc
     6301 cgcgcgcagg cgcgactcac ccgttcacaa acgtcccggc aggaccgcgc gccaacgcaa
     6361 ctcgatggcg actgcgagat gatccgagag ggcgcgacca ttcgagtccc tgaaccgtgc
     6421 atcgatgctc cacctcgtcg gagtgagctc cagcgcgctc gagctccgca ccaggaaacg
     6481 atcgatgcgc ctcggttcgg agcagcgcag cgccgtgcac acgtcgcgca accccaacct
     6541 cgattctaac gcggcgagtt cgtctcgctc cgaagcggtc aagttgaagt caccgcccac
     6601 gagcaaagct tgaccggaag actcgcggcg aattgcctct gacagctgcg ccaactgtcg
     6661 ggctcgagcg ctgcgatctg ccgcagacca ccccgcgtcg agatggacat tgtaaacgtg
     6721 taccactgct ttggaagcgg agtctccaag cacgagcgta gtgcgagaga agcccttggg
     6781 cgttaaacag tcgaagtacg aatccaccac accatgacaa gacgtccagg cggtccggga
     6841 tggttcggcg aatggaagct tggcgaatgt gctcaagccg tctccgaagt gcagcttgtc
     6901 tccgcgcacg aacggcgcgg acgcataggg tagggccagg tttttgcgga gcagctctgg
     6961 gtaggcgtaa tcctcttgga ttaaggccaa atcgtaagcg gcgaggtgcc cggcaatcag
     7021 cgggaggttg acgatgggat gaacgttgga ccatccctcc ggcaggcccg ccacgttgta
     7081 cgtcactacg cgcagcgttc ccgccccgcc ggaggtcggg ctggcccggc ttgcgagctg
     7141 cgtcttggcg ctcgccggct cgttcgtcgg gctcttggat tcagcctgcg tgggcccggt
     7201 caggcaggtg acgagacttg cggcggaaag aattgtggtc cagcgcttct gccccgaccg
     7261 tagctgactc ccccgcccca gcgctcctac tgagataggt tctaagccga tcgaggtgct
     7321 cttccgattc cgagttgacg ctccactcgg ctcccgaact tcgaccatac gtcagtttag
     7381 cagacgagcg gattctgccg ccgcggcgct ttgcctcgaa ggcccgtaca ctcgacctgc
     7441 ttcgcccgaa ctcgacggca cgtggtttgc tgcatcgttt gccgctgtaa cccaggaacc
     7501 gaaaccgcac gagaactggc gaggcgaaca tgaccacgag gcaccagaac gacgcacatc
     7561 gatcaacgcc gcttgggccg gctacgacca agaaccaaca cgctaaagag gccaagccgc
     7621 gcaccgaaca acggcgtgag gccgcctatc gaggtgaggc gaacgaggag gcctcgaaat
     7681 caccgcacgc gggacaaccg gatggccgca actaccgtga tcgagatggc aacgccggtg
     7741 gatcgcatgc ggccgcgtcg ccccccactg gcaagacgaa gttgccgagc tctcacgcga
     7801 atggtgcgtc tgactcccgc gccgactcgc aaggtcacga atcgctgagc caagagagtg
     7861 acgctcggaa tcgagccgca cgcaaggatt tgcgcaaatg agtaccgcaa agaacatcga
     7921 ctcgcagagc aaaccactag acccgcggga ggcagggccc aagccgccat tccccgagca
     7981 agagcaggat gaaaccggca gcgagcggag gatgcgtccg gcaccggatt atggtgccaa
     8041 ttatcgaggc tatgctcgtc tcaaggaacg ggtggcgttg attaccggtg gcgacagcgg
     8101 tattggccga gcggtggcgt tcgcgtttgc tcgcgaaggt gctcacgtgg ccatcaacta
     8161 tctcgagcac gaagaggacg cgcgagacac cgaaggccta attcgccagg cggaggcccg
     8221 gtcgctcgcc atccaggggg acatcggtga ggactcgacc tgcaagcgca tcatcgacga
     8281 ggtcgtgcgg gaattcggac acatcgatat cctggtgaac aacgccgcga agcagggaaa
     8341 gtcagtcgaa aagttcgagg agctcagcgc ggagagagtc gaacagacct ttcggacgaa
     8401 catcatatcg atgttcaacc tcgtccgaca cgcgctgccg cacatgcgcc ccggcgcggt
     8461 gattatcaat acggcatcga tccaggctta tcatccgtcg gccgagatat tggattacgc
     8521 caccaccaag ggggcgatcg tgaccttcac caagggcctc gcacaatcgc tcatcgaacg
     8581 cggcattcgc gtcaactgcg tagctcccgg cccggtttgg acccccctga tcgcgcagtc
     8641 cttcgacgcc gagaaggtgg ctcacttcgg tgagacctca ccgaccgggc gccctgcgca
     8701 gccagcggag ctggcgccgg cttacgtctt cctggcgagc gatgaatcgc gctacgtgac
     8761 cggcgaagtg ctaggcgtca ctggcggtaa gcccttgggt tgattgtgcg gctcgtggaa
     8821 cgggaggggg cggcctcgac cgtagctccc cgctgcgggc tgcgtactgc ggagctccgc
     8881 agtgcgcagg gcgaaacaag tcctgcaggg ggctacgcgg acgcgagtgc ggctatacgt
     8941 tgggctgccg ccaaagcgac attgcgtgcc actcgaggac ttcgttgtta gacatccgca
     9001 gaatataggt gtggcatttt ggtgttcgat tcgaggccaa cgagctcgcg ttgtagttat
     9061 acagggggcg ggtttgaggc gtgacttcag cctggctcca gattaagacg ggctgatagc
     9121 tctatggcag tcccgaagcc ccgagtagcc caagattccg gggagtttct cgcgggtggg
     9181 gggtagatgg gtgaacgcat gcgggccgtg gactgggcga aaacgccact tggtcccgtc
     9241 gagagctggc cgcgcagttt gaagacatgc gtccgtatcg tgctcacctc ccgacagccg
     9301 atgttcgtgt ggtggggccc cgagctcatc aacctgtaca atgatgctta caaaacgatt
     9361 gtaggtggca agcacccgca agcgttgggt cagccggctt cggtcgtatg gagagagatt
     9421 tgggatcagg tctcgccgcg tgctgagaag tccatgcggg gtaacgaggg tacgtacgac
     9481 gaagccttgc tcctgattat ggagcgcaac ggctatccgg aagagacgta ctacacgttt
     9541 tcctacagcc cggtaccggg agatgatggc caaacgggcg ggttgatctg cgcaaacacc
     9601 gatgacacgc gcaaaataat cggtgagcgt cagctagctc tgttgcggga cgtggcggcg
     9661 cgcacggccg atgcgcgcac gatcaccgag gtttgcggcc gcgctgccga agcaatggcg
     9721 acaaaccctc gtgatctcgt gttcgtcttg atttacttgc cggactcaca agtctcgggc
     9781 gaggtgctta cgctctccgg cgcctccggg atcgagccgg gacacccagc cgcgcccctg
     9841 cggttgcggg cggagcttgc tcgcggaacc ttggccgagg ccgctccata tccggtcgcg
     9901 gaactcttga agagctcggg cgcgcgcagc ctagagctgt cagagcaaaa gctgggtccg
     9961 ctcccgtgtg gcgcgtggga tcgtccgcct gcgcgcgccg cggggcttcg catcccattg
    10021 agcagcgggc gttccggttt gctgatcgta gggctcaatc cctttcgttt gttcgattcg
    10081 agctaccaag gcttcctcga gttgattgcc tcacaactcg cggccgggat cggggtgacg
    10141 caagcttacg aggacgagcg gcgccgcgcc gaatccttgg ctgagctcga tcgggcgaag
    10201 acggccttct tcagcaatgt cagccatgaa tttcgcaccc ctctcacgtt gattctgggg
    10261 cccatcgagg acgcgctcac gaacgcaccc gctcttttga ccggcgagaa cctgcgcgcc
    10321 gtgcaccgaa atgcgctgcg gttgctcaag ctcgtgaatg ccctgttgga cttctcccgc
    10381 atcgaggccg gccgcgcacg cgcggagttt cgtcttacca acttggcagc tttcacggct
    10441 gacgtggcca gtgcattccg ctccgccatc gagcgcgcgg ggctgcagtt ccagaccgcg
    10501 ttttctaagt tcgatgacga agtctacgtc gaccacgata tgtgggagaa aatcgtcctg
    10561 aacctgctct cgaatgcgct caagttcacg ttcgacgggc agatccgctt gagcctgcag
    10621 ccgcgcgggc aattcgcgga gcttacggtg gaggacaccg gtactggcat tcccgccgat
    10681 gcgttaccgg acttgttcaa aaggtttcag cgtgtccaag gcgcccgcgc ccgcacgcac
    10741 gaaggctccg ggatcggact tgcactggtc cacgagctca ttcgtctgca cggcggaaca
    10801 gtcggcgtgg agagcgaact cgggcggggc acacgctttc gagtgctgat tccgttcggg
    10861 agtaaccatc tgccctcgga gcaagtgagt tttacgcccg cgagcgccaa cctctccaac
    10921 gcgcccccgt tcgtgcaaga agcctatcgc tggttggcct tacccacgga ctcgccggat
    10981 tcgctgactc caacccagct gtcgcgcgac accaatcgat cgacgccgcc tccggcgttg
    11041 cccctgccgc gcgaggcccc gcggatcctg atcgtggacg acaacgcgga tatgcgagag
    11101 tatctggccc gcctgttgtc ggaccgcttc atggtcgaga cggccacgga cggggcggcc
    11161 gcgctggtcg cgattcggcg ctcgcctccg gctctgatct tgagtgacgt gatgatgccg
    11221 aacctagacg gcttcggcct gttgcggatt ctgcgagcgg accccgcgct cgcgaggacg
    11281 cccgtcatcc tgctctcggc gcgcgcgggt gacgaagcaa gcgtcgaagg cctgaccatg
    11341 ggtgccgacg attatctcgt caaacccttc tccgcgcgtg agctcttggc gcgcgtggag
    11401 acgcaccttc aacttgcttc tttgcgcaac tcggccgaac gtgagcgggc gaagttgtat
    11461 gccgtgttca tgcaggcgcc cgttccggtc gccgtgctga tgggggccga gctgcgctac
    11521 gaggtagcga atgcccacta ttgtgaggta gtggggcgca acgtcttgaa ccaaactttg
    11581 cgggattctt ttcccgaact cggggatcac cccatattcg agagactcga aggtgtttac
    11641 aagaccggcg aagccgttca tgaccccgag ttgcgggttg cgctctatcg tcgagggttg
    11701 caggaagatg cgtatttcaa ctacgtggcc cagccgctgc gcgatttgga cggtagagtc
    11761 gcgggggtaa tcatcgttgc ttccgatgtg accgaccaag tgctcagtcg ccagcgcgtc
    11821 aatgaactcc gctccacggc ggagagtgcc agccgcgcta aagacgagtt cttgagtacg
    11881 ttgagccacg agctgcgcac gccgctcagc gcgatcatcg gctggtccag catgttggtg
    11941 tcgggaaacg ttcggcccga gcagcgtcag aaggcgctcg agaccatcga gcggaacgca
    12001 cgggcccagg cccgcttgat cgaagacatg ctggacctat cgcgcatcga gcagggcaag
    12061 ctcgtgctca gcgtggggcc ggtcgaggtc attcgcgtcg ccgaggccgc gattgaggcg
    12121 gtaagacccg ccgccgaggc caagggcatt cgcctgcagc ccgtgttgga ctcgcacgcc
    12181 accatcatcg gggacgccga tcgcctgcag caggtcgttt ggaacctcct gtcgaatgcg
    12241 atcaagttca cgcaaaaagg ggggcgcgtg cagctccgcg ttcgcaaaga ccgttcccac
    12301 gtagagatca ccgcagcgga tgacgggcaa gggatcgagg cggcattttt gccttatgtc
    12361 ttcgatcgtt tccgccaggc cgatccatcc ttcaatcgcc gcgccggggg attgggcttg
    12421 gggctggcaa tcgtgcgctc tttggtcgag ttgcacggcg gaaccgtgtc ggcgcacagc
    12481 gatggccacg gccgtggggc caccttcgtc gtgcggctgc caatggcgcc gttgcgcgcg
    12541 gatcgtgtct cacccgtgcc ggaacagagc cccaccgagc agcgcaagag tttcgaatgc
    12601 ccgcctgcgc tcaaggggct aaaggcgttg gtcgtcgacg atgagccgga tacccgcgct
    12661 ttgctttgct tcctactaga acagtgtgag acgagcgtca ccgcggtggg cggcgcgctg
    12721 gaggcgctcg cggagctgca gcgggccaat tacgatgttc tgatatcgga catcggtatg
    12781 cccgagctcg acggttacga gttgattcgc cgggtgcgca agttgccggt ggacagcaac
    12841 gggcgaattc ctgccattgc actcacggcc tatgctcgcg ccgaggaccg cacgcaagcg
    12901 ctgaagagcg ggttcaatat gcacctcgca aagccgattg aacccggtga actattggtg
    12961 gtcgtggcca cggtcacgaa cggctatcaa acgctcgagt cgtgatgctc ctcgggccac
    13021 tcgcccgtag tcgccttcag tcgcgccgag tgtgagggaa ttaggatctt ctcttgttga
    13081 agcggctcgc caagaaaagt ccgagcgcta acccgagcag ccccaagggg ctgccgtcgc
    13141 gcgcgggcgg cttcatggcg cagcctccac gcgccgccgc ggcggcggtc gacgggttca
    13201 cggtgacgct cccttccacg ttgaccttgg caataccgcg ctcggacaga gcggtggcac
    13261 acggcacgat atcgctggcg gatagtacgt attcaccgtc gcagaacaaa gcgccctgtg
    13321 cgttacacga tgcgctgcag tctgccttga gctcgtattc gcagctctcg aattgctcgt
    13381 cttggcaggt ggtttgacaa tccatgttgg cttgcgcggt gcacgcgcca ccgcagcatt
    13441 cgatacagtg attgtaacag gaggcgtcca cgagggctcc gcactgcatg tcacactctc
    13501 cgtcgcacgt ggcctcgcac gtggcgacgc actgctctgg atcgggatgc ccggcacaac
    13561 gatcgctgca gtttcccgcg cactcggtga agcagttgtg aatgcaggtg accggcactc
    13621 cgcgatcgca ctcctgttta cacttcacgg tgcactcgtc gctgcagctg ggttctgcgg
    13681 ccagcgtgca ttcgctgcga cacactttat gcagcttcgt agcgcacgcc ttcttgtaaa
    13741 ccccgagctc gctacatcca ccctgacaac tcacgctgcc gcgcacttcg cagcgcctca
    13801 cgtcctcgag tcgcagccct ccgcactccg gaacaccagc ccaggcctgc gtggagccga
    13861 acagaaccat cgcgccgagc agcccggcct tcgcaaagcc gaggttccac ttcgatgcgg
    13921 gcattcgctc tcgcgcacgt attccgacca tcgctctttc ttgcattgat gctcccatga
    13981 cccctcggag ggtggatccg gcggcgctgc ggcgaccggg agttgcggca gcgtcgttgt
    14041 gcaaaagctc tgccattggt gccccactgc gcctcgcgcc acggacgacc cagcccgacc
    14101 ggcgatacca cctaaatcgc gcggacatcc gaatcggcgg gcgcttcggt gctctgatct
    14161 cgatgtcgcg gtgacgttgc ggaaggtcga gctcgaggcc gcgccggggg cggcgagact
    14221 ccgttgattg gctgacttcg tctaacgacc tgtgaagggt cgtttgggta cgggccgggc
    14281 gggcaggggt cgaggaatcc aaccgccgtt ctcggaaatg aactggcaat ccacggtctt
    14341 gagccgcttc atcacacgcg aggtcgtccg gtatagagag ccggaactat cggccttctt
    14401 cagaaagacg agcaactccg cctgcccaac gatggtgctg aagcgcggcc ggtacacgtc
    14461 ccgtcccaca caaacgagcc ggtaaacctg gagagcccag cgcgagttct cgttgatgag
    14521 acgcacgggg atggatccgt cactcagaca catgaaacac aggagctttc tgcaagggtg
    14581 gccaccgctg aaacgtcgaa attaggagct aattaagtga ttgcggcgtg gcatttgggc
    14641 gctttgagca ttgtctaagg ctgctgggcc cttggattgc gtgccgcgtc gacgccaatc
    14701 ggtgcggatt gcggtgcttg accgacgcct ctttacgtca acgactacat a
//
LOCUS       DASTKU010000811        11892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1947969, whole genome shotgun sequence.
ACCESSION   DASTKU010000811 DASTKU010000000
VERSION     DASTKU010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11892
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947969"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(657..1052)
                     /locus_tag="VFQ61_28360"
     CDS             complement(657..1052)
                     /locus_tag="VFQ61_28360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958454.1"
                     /translation="MSKIFEYGFTLAAVFSIAACSGEATEPVPDVTGKWCGATVASAA
                     ECQGDEVGYLDIVQTGDRVTGKACEAYEKDCMVLQDARVQGKELTFFYTFLEYRVDAK
                     LAISRDTLSGSYVSDKCNCEIPFTFNRVR"
     gene            complement(1125..1556)
                     /locus_tag="VFQ61_28365"
     CDS             complement(1125..1556)
                     /locus_tag="VFQ61_28365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958455.1"
                     /translation="MQFEAVLITVGLLVGSASLAGCGEVEEDNPDTNSGGGLGASGVL
                     ASKALGSLDPAERAKLCDWSHLVTGGYGQSYYCPDGTRIDTDENQEDCLKALQETLKP
                     EVKVTIREYEACINEYAKDTCDFSTLSTAPACKGLLPWFAP"
     gene            1858..2802
                     /locus_tag="VFQ61_28370"
     CDS             1858..2802
                     /locus_tag="VFQ61_28370"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET9958456.1"
                     /translation="MVENSSGMAALVRTERGVELLRMERPAPLPANHVRVRIERAGLC
                     RTDLNVASGRLRCEWPRVLGHEVAGIVSELGARAARVRLGQRVTVNPLLPCLRCAECA
                     RGEVCEAPRMLGVDQDGGFSEELVLPESAVFPVPDSMSFERAAYVEPVAAAMAVLDCP
                     LDREGAGLIVGSGRIAELTRRVLLAQGFPSPHVVASEGAGPQRGFAWAIDTVGTTESL
                     ALASRALRQRGLLVLKSRPAELAGLDIRFAVERELVLRAVRYAPFEHAIELLASPRFE
                     VEDLFGGTYPLAEFERVFAEAARSEGSKLFFALDAGSR"
     gene            2802..4718
                     /gene="asnB"
                     /locus_tag="VFQ61_28375"
     CDS             2802..4718
                     /gene="asnB"
                     /locus_tag="VFQ61_28375"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET9958457.1"
                     /translation="MCGVVAIIGQAQRWSEPERRRALDALAHRGPDGMGNYLSPDGRV
                     WLGHTRLAVIAPQDGAQPVANEDGSIVASVNGELYGFASLRTELEAMGHRFLTQSDSE
                     LVVHLYEEYGLEAVRHLRGEFAFVLWDRARGRAVAARDRFGIKPLVYTEQAGSLLVAS
                     EAKALFALGFGAAWDRSSLEHAFAHQYLPPNRTLFRGVKQLPPGHLLIAECGTDGALG
                     AVRIERYWDLDLPRTERAVDLEDAAEKLLSQLEQAVKLRLRADVPVAFHLSGGLDSAS
                     ILALAAQHSSQRLHAFTVAFQHPPYAEGELARDLARELGVELHVVDVPQSALLEALPD
                     AVFHGEGLCINGQLPSKLLLARAIRKAGFTVALSGEGADEALLGYAHLASDLVEAETV
                     APNDTHSAQVGVMLPSADAPDLPEVQARLGFVPTFLRAKAAFGRELLALTTEPDAGVA
                     IRQRAFERLFESLGCEDQLSERAPVLQSAYLWTKLALAGYILRTLGDGTEMAASVEGR
                     TPFLDHHFYEFVRELPVSAKIRMGTNGVTQKYLQRVALRKLLPEAVLTRTKQPFLAPP
                     LTRFVSREARELVQDTLSDRALGEIPYLHVRRTRNFLERCFQSSDRTKNSDDAVLMTL
                     LSACYLQQRFALTG"
     gene            4725..5558
                     /locus_tag="VFQ61_28380"
     CDS             4725..5558
                     /locus_tag="VFQ61_28380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9958458.1"
                     /translation="MNDLRIHAPSEAPPSGVWWMDLYDDLLADVFLEREEEVADTLVF
                     LRRVLGLEPGMRVFDQCCGIGRLAIPLGQQGFVACGCDVIPRYIERALEKAQTAGVCV
                     ELATADAFEFTTSVPCDGAFNWWTSFGYVQEDAANMRMLQRAFESLKPSARFALDFMN
                     VPGVLRGFQARTETVRETPWGTVQLVRESQIELREMVMLKKWRYDFRAERTTEDITDR
                     KANRRTVQHHSRVRLYLPHELIALFERAGFGDIELYGDLAGNPLGLDSPRCIVVGRRP
                     E"
     gene            5555..6391
                     /locus_tag="VFQ61_28385"
     CDS             5555..6391
                     /locus_tag="VFQ61_28385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958459.1"
                     /translation="MNETIREETPANPAELRSALYAGDLFKLAPNATSLEMVAAVEQL
                     LDVELGPEPRQAASRMTDEEHFERVGRIRKQLFLQPSYHELVFRLLESLGQPRTGIAF
                     DPLRLRCVLDGGHDNPRARAVYYPHRDTWYGHPAALITVWIPIYDLTAEETFVFYPER
                     FTTPVPNNSEIFDYDEWVERGWGLKIGWQNRNAGIEARYPGVLGEVEPGPALGFSCRR
                     GEILLFSGAQFHKTLPQRLGRTRFSLDFRFVSLADHEQGLGAPNVDSRSRGSALRDYV
                     RA"
     gene            6402..9362
                     /locus_tag="VFQ61_28390"
     CDS             6402..9362
                     /locus_tag="VFQ61_28390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9958460.1"
                     /translation="MAGSLERFLTIAKQHADRPAIVEQSKRGTTTLLYGELRARAERL
                     GSLLMERGVGPEQIVALDLPKSADLVMAALACWYAGAAFLPLDWSLPSARRAAYLRQA
                     NVSLAITTEAESRVPEGVPCLVAETRLATRTLPAAQFDAERLAYVISTSGSSGIPKAV
                     AVSHRGLLPLLEAQIRAFDLEPGDCSSWLLSPAFDASISDWGTALLSGATLYIDPRLP
                     GDGPRAIQEAISRDKISYIDLPPALLPHLPVGPYPSLKTIVIGGEVAEVAAVRRWSER
                     VRLINVYGPTETTICTSYRVADATWEKPLLGQPIDRSLVQYAVRDPEGRRCAPDTPGE
                     LYISGACLARGYLAQPELTQTRFVEIEDARAFRSGDRVVQRSDGEFEFLGRIDRQIKV
                     RGALVAPEEIEAELLSIPGVRRAAVLEVGERKRITAFLEGERTADSELRCQLAARLPH
                     YMVPERFEWLDVMPLTSSGKPHLVELQEPRGSRVTATSPPEEEHRGLKERIAAWYARV
                     LGTDVEPEDDFFALGGNSLRALELIALAALDGVELTPDLLFKSPRVVTLAQALTSRDS
                     SNGKSCHELTTRLHTEGLLSRPDSWIGSEEKQRARFASEDPDSVLVTGATGFLGSRLI
                     LELLEQTKSQIWCLVRAPDPVTAETRVLDAIASHGRGAVDARRLRCVVGDLSEPTLAR
                     ADSDRRGLVDDIHTIYHCGAAVSLMADCNALWETNVRGTDRVLRLCERGRPKSLHYAS
                     TLSVFVSARSSQVSARHEYLESDCLEQTLEVFGGYAQTKWAAEVLVRTRANPRSSTFI
                     HRLGLLTGDSRTGRGAERDWLALVLRGLTKLGIYPQSYDAGLAVDITPVDYVTRALVH
                     LARQATRPGHVQTTHLAGPRAVGFGELVSALQGAGVHLEPVEPNIWATRLSEALESSA
                     EPELASAFLALRRVSAGERAQHALDLFQATRCRFDDRETRTLLSPVGIECPPATPALL
                     REYARHTSSTERAS"
     gene            9359..10351
                     /locus_tag="VFQ61_28395"
     CDS             9359..10351
                     /locus_tag="VFQ61_28395"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9958461.1"
                     /translation="MSLRWGRGLVLGKFMPPHRGHQYLVDFARHAVERLAVVVEHVRN
                     EPIPSQLRYAWMRELCPGCDVVHLQEENPQQPSEHPDFWNIWRSSLERVLPYSPDVVF
                     ASESYGPRLAAVLGAEFTPVDPGRNIVPVSGTAIRQAPLEHWQYLPECVRPYFVKRVC
                     IFGPESTGKSTLTRQLADHFGSVAVPEYARTLLEHQAGQVRAEDMMRIVRGQVASEEA
                     LARRAQRVLFCDTDPLTTSIWSDVLFGACDPRVLAEASSRHYDLTLLLDVDVPWQRDV
                     VRYLPEQRHAFFERCRAALDSEHRDYVVVRGAWQERWEIAVGAVSRLLGSPCSD"
     gene            10538..>11892
                     /locus_tag="VFQ61_28400"
     CDS             10538..>11892
                     /locus_tag="VFQ61_28400"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HET9958462.1"
                     /translation="MSSAWRLEELSKRPRNTARHVTPGENMSERHQYLLDESRLPKAW
                     YNVNADLPVPPAPVLHPQTLAPITPDYLSAILPMNLIEQEASRERYIEIPEPVREIYK
                     LWRPTPLMRAVRLEKALGTPAHIYFKYEGVSPVGSHKPNTAVAQAFYNKVAGTKAIAT
                     ETGAGQWGAAMAMACNFFDLDLEVYMVKVSYEQKPYRRMVMENFGARVYASPTDRTHA
                     GRAVLATDPTSPGSLGLAISEAVEVAATSGGAKKYALGSVLEHVLMHQTVIGEEALLQ
                     MELAGEYPDVVIGCAGGGSNFAGFVFPFVREKLRNGLKTRIIAVEPTSCPTLTRGVYA
                     YDWGDQAKTAPVVKMHTLGHGFMPPGIHAGGLRYHGMAASVSALVDHGDIEARAVNQL
                     ETFAAAVQFSKAQGIVPAPESAHAVRAAIDEALNCKLTGEPKVIAFNLSGHGHFDMAA
                     YS"
BASE COUNT         2083 a         3567 c         3894 g         2348 t
ORIGIN      
        1 gagggcaaag agggtgtccg gcgccttcac ctcgtcgaag cgcaccagat ggcggtgtga
       61 ggagcgtccg ctcctccgtc gcgctcttgc ctgttattat ccgccggtga cacgcgaaga
      121 aagggccgct cagcgtcgat cgcaatggac ccaaggcctt gtctcggcag ccgaggcaaa
      181 caatctcgat cgcacattct ggagccagac gacagcagcg aaccgcctcc aggcagtctg
      241 ggagatggcc aatcaatggt ggaacaacga gcaccccgat ggacctcccc tccgacttga
      301 tagatctgct ttcggcattc gccgaagagc aggttgaata ccttctcatt ggcggccagg
      361 ccgtggcgct gcacggggtt ccgcgtttca cgacggacgc tggcttgtgg ttgcatgaca
      421 cgagcgaaaa cctggagcgc gcaaagcgcg cccttgactc gttcggggct cctgcgggca
      481 ccctccgcgc gcaaggcggc cctgatcgca caaaagcgcg cttccggacg cccgcaagat
      541 ctagtcgacg cggacaatct cgagcgcggg tagcgaagtc acgaaacgct gcgccttcta
      601 cgccgcgagc gttggacatg acgcgagctc acgcctgaag gtctcgagaa ggtttgctac
      661 ctcacgcgat tgaacgtaaa cgggatctca cagttgcact tgtcactgac gtaggatccc
      721 gagagagtgt cgcgcgagat tgcgagtttg gcgtccactc gatattcgag aaatgtatag
      781 aagaacgtga gttctttgcc ctgcacgcgc gcgtcttgca gcaccatgca gtccttttcg
      841 taggcttcgc aggctttgcc ggtgacgcga tcgccggttt ggacgatatc gaggtaaccg
      901 acttcgtcgc cttgacactc ggccgcgctc gcgacggtgg ctccgcacca tttgccggtg
      961 acgtcgggca cgggctcggt cgcttctccg gagcaagctg cgattgagaa cactgcggct
     1021 agagtaaagc cgtattcgaa gatcttcgac attgaattcg tgcgtggcac ggcggagcag
     1081 cccgcgcaac caacaccgcc gtgctcgagc cgtagcgagc cgaatcaggg ggcgaaccag
     1141 ggcagcaacc ccttacacgc tggcgcggtg gataaagtgg agaagtcaca agtgtcctta
     1201 gcgtattcat tgatgcacgc ctcgtattct cgaatcgtca ccttgacctc gggctttagc
     1261 gtttcttgca gtgccttcag gcaatcctcc tgattctcgt cggtatcgat tcgggtgccg
     1321 tcaggacaat aataagattg accgtaacct ccggtcacga ggtggctcca gtcacagagc
     1381 ttcgctcgct ctgctggatc gaggctgcct agagccttgc tcgcgagcac gccgctggct
     1441 cccagcccgc ccccgctgtt cgtgtcaggg ttgtcttctt cgacctcacc gcaacccgcg
     1501 aggctcgccg agcccaccag aagacccacc gtgataagga ccgcctcgaa ttgcatggct
     1561 tacactcccg tatggctctt gctttgggcc gctgaatcgc acgtcaccgc cccatcttgc
     1621 agacaatgtc tcgaaaaagc gagcgggtgc cctggatcgt gacgctgtga gctttacttc
     1681 gtgccgcttg cgcggtgact tttcgtgaag ggagcgtagc ggctgcgctt gggtgaggat
     1741 gcgacgggtt tcgcgcccgt cgacggaggg cggtcaatcc aatggctcgc ttgcccgcgc
     1801 gggcttctgt tgtacgctgg catcggcgtg cgcggtggtc agatgaatag gtcctcgatg
     1861 gtagagaatt cgagtgggat ggccgcgctg gttcggaccg agcgaggggt ggagctgttg
     1921 cgtatggagc ggccggcgcc gctacctgcg aaccacgtgc gagtacgcat cgaacgtgca
     1981 ggattgtgtc gcacggactt gaatgtggct tcgggtcggc tgcggtgtga gtggcctcga
     2041 gttctgggac atgaggtggc agggatagtg agtgagctgg gcgcgcgcgc ggcgcgcgtg
     2101 cgcttggggc agcgcgtgac ggtgaatccg ctgctccctt gtttgcgttg tgcggagtgc
     2161 gcgcggggag aggtttgcga ggccccccgc atgttggggg tggatcagga tggtgggttt
     2221 tcggaggagc tggtgctgcc ggagagcgcc gtgttcccgg tgccagactc gatgtcgttc
     2281 gagcgcgcgg cttacgtgga gccggtggcc gccgcaatgg cggtgctgga ttgtcccttg
     2341 gatcgggaag gggcgggctt gattgtgggc agcgggcgca tcgcggagct gacgcggcgg
     2401 gtgctgctgg cgcagggttt tccaagtccc catgtggtgg caagtgaagg ggcggggccg
     2461 caacgcggat ttgcttgggc gatcgatacg gtcggtacga cggagagctt ggcgttggcg
     2521 agccgggcct tacgccagcg cgggcttttg gttttgaaga gccggcccgc ggagcttgcc
     2581 ggattggata tccgatttgc ggtggagcgc gagctggttt tgagggcggt gcgctatgcg
     2641 ccgttcgagc acgccatcga gctcttggct tcgccgcgct tcgaggtcga ggacttgttc
     2701 ggcggcactt atccactcgc ggagttcgag cgcgtgtttg cggaggcggc gcgttcggag
     2761 ggttcgaagc tgtttttcgc tctggacgca gggtcccgct gatgtgtggg gtggtcgcga
     2821 tcatcgggca agcgcagcgc tggtccgaac cggaacgccg acgggcgctc gacgccctcg
     2881 ctcaccgcgg acccgacggg atgggcaatt acctgtcacc ggatgggcgg gtgtggctgg
     2941 gtcatacgcg attggcggtg atcgctcccc aggacggcgc gcaaccggtg gccaacgaag
     3001 acgggtcgat cgtggccagc gtgaacggcg agctctatgg gttcgcgagc ttgcggaccg
     3061 agctcgaggc gatggggcat cgttttctaa cgcagagtga cagtgagctg gtcgtgcact
     3121 tgtacgagga gtacggcctc gaagcggtgc gtcatttgcg cggtgagttc gcgttcgtgt
     3181 tgtgggacag ggcacgaggg cgcgccgtcg cggcgcgcga tcgcttcgga atcaagcctc
     3241 tggtgtatac ggagcaagct gggagtttgc tcgtcgcctc ggaggccaag gcgctcttcg
     3301 cgctcggttt tggcgcggcg tgggatcgct cttctctcga gcatgctttt gcgcatcagt
     3361 atttaccccc gaaccgcacg ctgtttcggg gtgtgaagca gctcccgccg gggcatctgt
     3421 tgatcgccga gtgtgggaca gatggcgcgc tcggtgcggt gcggatcgag cgatactggg
     3481 atttggattt gcctcgcacc gagcgcgcgg tggatttaga agatgccgcg gagaaactgc
     3541 tcagccagct cgagcaggcc gtgaagctgc gcctgcgcgc ggacgtgccg gtggcgtttc
     3601 acttgagcgg cggattggat tcagcgtcca ttctcgcact cgccgcgcag cactcgagtc
     3661 agagattgca cgccttcacg gtggctttcc aacacccgcc ctatgcggaa ggcgagctcg
     3721 cacgcgacct ggcgcgcgaa ttgggcgttg agctgcacgt ggtagacgtt ccgcaatcgg
     3781 ctctgctcga ggcgctgccg gacgcggtgt ttcacggcga gggactgtgc atcaatgggc
     3841 aactcccgag taagctcttg ttggcgcgcg cgatccgcaa ggcgggcttc acggtggcac
     3901 tctctggcga gggcgcggac gaagcgctac ttgggtacgc acacttggca agcgacttag
     3961 ttgaagctga aactgtcgca ccgaatgata cccactctgc gcaagtggga gtgatgttgc
     4021 cgagcgcgga tgctcccgat ttgccggagg tgcaggcccg tttgggtttc gtgcccacgt
     4081 ttttgcgggc caaagcagcg tttgggcgtg agctcctcgc gctgaccact gagcctgacg
     4141 ccggagtggc catacgacaa cgagccttcg agcgcttgtt cgagagcttg ggttgcgaag
     4201 atcaactgag tgagcgagca ccggtgctgc agtctgctta tctctggacc aagcttgcgc
     4261 tggcaggtta catcttacgc acgttgggag atggaaccga aatggcagcc tcagtcgagg
     4321 gccgtacgcc gtttttagac caccactttt acgaattcgt gcgggagttg ccggtgtccg
     4381 cgaagatccg gatgggcacg aatggcgtga cccagaagta cttgcagcgg gttgccttgc
     4441 gcaagctgtt gccggaggcc gtactgacgc gaaccaagca gccgtttctg gccccgccgt
     4501 tgacccgctt cgtgtcccgt gaggctcgcg agctagtaca agacaccctg agtgatcgcg
     4561 ccttgggcga gattccttat ctccacgttc gcagaacccg aaactttctg gaacggtgct
     4621 ttcagagttc ggatcgcacc aagaattccg acgatgccgt gctcatgacc ctgctttccg
     4681 cctgctacct gcagcaacgt tttgcgctga ctggctaaag actcatgaac gacttgcgca
     4741 ttcacgcccc gtccgaagca ccgccaagcg gtgtctggtg gatggacctc tacgacgacc
     4801 tgctcgcaga cgtgttcttg gagcgcgagg aggaggtcgc agacacactg gtttttctcc
     4861 ggcgggtgct ggggctcgag ccgggcatgc gcgtcttcga tcaatgttgc gggattggca
     4921 ggctcgccat cccgctgggc caacagggct tcgtggcgtg cggctgtgac gtgattcctc
     4981 gttatatcga gcgagcgctc gagaaagccc agaccgcggg ggtttgcgtg gagctcgcga
     5041 ccgcggatgc gttcgagttc acgacttcag tgccgtgtga cggtgcgttc aattggtgga
     5101 cgagttttgg gtatgtgcaa gaagacgcgg ccaatatgcg tatgctgcaa cgcgcgttcg
     5161 agtctttgaa accgagcgcg cgctttgcgc tggatttcat gaacgtgccg ggggtgctgc
     5221 gcgggtttca ggcgcgcacg gagacagttc gggagacgcc gtggggcacg gttcaactcg
     5281 tgcgtgaaag tcagatcgag ttgcgagaga tggtcatgct caagaagtgg cgctacgact
     5341 tccgggcaga gcgtaccacg gaggacatca cagaccgcaa ggcgaaccgt cggaccgtgc
     5401 agcatcacag ccgagtgaga ttgtacctgc cgcatgagct gattgcgctg ttcgagcggg
     5461 ccgggtttgg agatatcgag ttgtatggcg atctcgccgg caacccattg gggctggaca
     5521 gcccgcgctg cattgttgtg ggccggaggc cagaatgaac gagaccattc gagaggagac
     5581 acccgccaac ccggcggagc tgcgctcggc gctctacgcg ggggacctat tcaagctggc
     5641 acccaacgct acgtcacttg aaatggtggc ggccgtggag cagctgcttg atgtggaact
     5701 tggccccgag ccgcgacagg ccgcctcgcg catgaccgac gaagagcatt tcgagcgcgt
     5761 ggggcgaatt cggaagcagc tgtttctgca acccagctac cacgagctcg tctttcgatt
     5821 gctcgaaagc ttggggcagc cccgtacggg aatcgccttc gatccactgc gcctccgctg
     5881 cgtgctcgac ggcggacacg acaatccgcg cgctcgcgcg gtgtactacc cgcatcgcga
     5941 cacgtggtat gggcatccgg ccgcgttgat cacggtttgg attcccatct atgacttaac
     6001 ggcggaagag acgttcgtgt tttatccaga acgcttcacg actcccgtac ccaataactc
     6061 ggagatcttc gactacgatg agtgggtaga gcgcggctgg ggcttgaaga ttggttggca
     6121 gaaccggaac gcggggatcg aagcgcgtta tcccggagtg ctgggcgagg tcgagccagg
     6181 cccggcgctg gggttcagct gccggcgcgg ggaaattctg ctgttcagcg gcgcccaatt
     6241 ccacaagacg ttgccgcagc ggctcgggcg cacccgcttc agcttggact ttcggttcgt
     6301 gtctttagcg gatcacgagc agggtctggg cgcaccgaac gtggatagtc gctcgcgagg
     6361 ctccgccttg cgggactacg tgcgtgccta ggcgaaagat catggcgggt tcgctcgaac
     6421 gctttctgac tatcgcaaag cagcacgcgg atcgacctgc gatcgtcgaa cagtccaagc
     6481 gagggacgac gacgctgctc tatggcgagc ttcgcgcgcg tgccgaacgc ctcgggtcat
     6541 tgctcatgga acgcggcgtt gggcccgagc agattgtggc gctcgacttg cccaagtcag
     6601 cggacctcgt catggccgcg ctcgcttgct ggtacgccgg agcggctttt ctgccgctcg
     6661 attggagttt gccgagcgcg cgacgcgcgg cgtacctgcg gcaagccaac gtctcactcg
     6721 ctatcacgac ggaggcagaa tcccgagttc ctgaaggcgt accctgtttg gtcgccgaaa
     6781 cgcgacttgc cacgcgaact ctacctgcag cccagttcga cgctgagcgg ctcgcgtacg
     6841 tgatctcgac gtccggctcg agtggcatac ccaaagccgt ggccgtgagc catcgtggct
     6901 tgcttcctct gctcgaagct caaattcgag cgttcgattt ggaaccagga gattgcagct
     6961 cgtggcttct ctcgccggcc ttcgatgcat ctatctcgga ttggggaaca gcgctgttga
     7021 gcggagccac gttgtatatc gatccacgat tgccggggga cggtccgcgc gccatccaag
     7081 aggccatttc gcgagacaag atctcgtaca tcgatcttcc tcccgcgctg ctgccccact
     7141 tgccggtcgg gccatatccg tcactcaaaa ccatcgtgat cggcggcgag gtcgcggagg
     7201 ttgcggccgt gaggcgctgg agcgagcggg ttcgtctgat taacgtttac ggccccaccg
     7261 agactacgat ttgcacgagc tatcgagtgg ccgatgccac ttgggagaag cctctcctcg
     7321 ggcaaccgat cgaccgctct ctcgtgcagt atgcggtgcg cgatccggaa ggccgccgat
     7381 gcgcaccgga caccccgggt gaactctata tctctggggc ctgcctggcg cgaggctacc
     7441 tggcacaacc cgaactcacg cagactcgct tcgttgagat cgaggacgcg cgcgccttcc
     7501 gatcggggga tcgcgtggtg cagcgctcgg acggagagtt cgagttcctg gggcgcatcg
     7561 atcggcagat caaggttcgt ggcgcactgg tggcgccgga ggaaatcgag gccgagctcc
     7621 tctccatccc aggagtgcgg cgggccgcgg ttctcgaagt gggcgagcgc aaacgaatca
     7681 ccgcgttcct cgaaggcgaa cgtaccgcgg attctgagct tcgatgtcag ctcgccgcgc
     7741 ggctcccgca ttacatggtg cccgagcgtt tcgagtggct cgacgtcatg cccctgactt
     7801 ccagcgggaa gccccatctc gttgaacttc aggagccgcg aggatcgcgc gtgactgcaa
     7861 ccagcccacc cgaggaggaa catcggggct tgaaggaacg cattgcggct tggtatgcgc
     7921 gggtgctcgg cactgatgta gagccagaag acgatttctt cgccctaggt ggcaactctc
     7981 tgcgcgcctt ggagttgatt gcgcttgcgg cgctcgacgg tgtcgagctc acgccggact
     8041 tgcttttcaa gagcccccgc gtggtcactt tggcccaggc attgacgagt cgtgactcgt
     8101 ccaatggtaa gtcgtgccac gagttgacta cccgtctaca cactgaaggc ctgctatccc
     8161 gtccggactc gtggatcgga tccgaggaaa aacaacgagc gcggttcgcg tccgaggatc
     8221 cggattcagt cctggtcact ggcgccaccg gttttctcgg ttcgcgactg atcctcgagc
     8281 tcctggagca gaccaagagt cagatctggt gtctcgtgcg cgccccggat ccggtcacgg
     8341 cagagacgcg ggttctcgat gcaatcgcga gtcatggtcg aggggcggtg gatgcccgac
     8401 gccttcggtg cgtggtggga gacctctcgg aacccactct ggcccgtgca gattccgacc
     8461 gtcgcgggtt ggtcgatgac atccacacca tctaccattg cggagccgcg gtgagcctaa
     8521 tggcggattg caacgcgctt tgggagacga atgtgcgcgg caccgaccgc gtgttgcgcc
     8581 tctgcgaacg gggccgcccc aaatcgctgc attatgcgtc caccctgtcc gtgttcgtgt
     8641 cggcgcgctc ctcccaggtc tcggctcgtc acgagtatct cgaatcggac tgtctcgagc
     8701 agacgctcga agtattcgga ggatacgcgc agaccaaatg ggcggcggag gtcctggtgc
     8761 gcacgcgcgc caacccgcga agtagcacgt tcattcaccg tctaggtctg ctcacgggcg
     8821 atagccgtac gggccggggc gccgagcgag actggctggc gctcgttttg cgaggtctga
     8881 ccaaactcgg tatttatccg cagagctatg acgccgggtt ggcggtcgat atcactccgg
     8941 tcgactatgt cacgcgtgcc ttggtccatt tggctcgcca agcgacgcga cccggacacg
     9001 tgcaaactac gcacttggcc gggcctcgcg ccgtaggttt tggagagctc gtgagtgctc
     9061 tccaaggcgc aggagtgcat ctcgagcccg ttgaaccgaa catctgggcc acgcgcttgt
     9121 cggaagccct cgagtccagt gccgagcccg agctcgcctc cgcattcctc gctttgcgtc
     9181 gcgtgagcgc gggtgagcgc gcacagcacg cattggatct atttcaagcc acacgctgcc
     9241 gcttcgatga tcgggagacg cgaacactgc tctcgccggt cggcatcgaa tgccctcccg
     9301 cgacgcccgc gctgctgcgc gagtatgccc gccacacgtc gagcacggag cgcgcgtcgt
     9361 gagcctgcgt tggggccgcg gcctggtgct cggcaagttc atgcccccgc atcgagggca
     9421 ccagtacctc gtggacttcg cgcgtcacgc cgtagagcgg ctggccgtgg tcgtagagca
     9481 cgtgcgaaac gagccgattc cgagccaact gcgttacgct tggatgcgcg agctctgccc
     9541 cggttgtgat gtcgtccatc tccaggagga gaatcctcaa cagccctccg agcacccaga
     9601 cttctggaac atctggagaa gcagcctcga gcgcgtgctt ccctattcgc cggacgtggt
     9661 gttcgcttcc gagagctacg ggccgagact tgcggccgta ctgggcgcag agttcacgcc
     9721 ggtcgaccca gggcgcaaca tcgtgcccgt gagcggcacc gcgattcggc aagctccact
     9781 cgagcactgg caatatttac ctgaatgcgt ccgcccatac ttcgtcaaac gagtttgtat
     9841 cttcggcccg gagtcgaccg gcaagagcac actcacccgc caactcgcgg atcatttcgg
     9901 cagcgtcgcc gtgccggaat acgcccgcac tctgttggaa catcaggcgg ggcaggtgcg
     9961 tgcagaggac atgatgcgca ttgtgcgggg tcaagtcgct tccgaggaag cgctcgcccg
    10021 ccgcgcgcag cgtgtcttgt tttgcgacac tgatccactg accaccagca tctggagtga
    10081 cgtgctgttc ggcgcctgtg atccgcgtgt tctcgccgag gcgagctcac gccactacga
    10141 tctcaccttg ctgctcgatg tagacgtacc ctggcaaagg gacgtggtgc ggtatctgcc
    10201 tgaacagcgt cacgccttct tcgagcgctg ccgggccgca ctcgattcgg agcaccggga
    10261 ctatgtagtc gtgcgcggcg catggcagga gcgatgggag atcgccgtag gcgccgtatc
    10321 tcgactgctt gggagccctt gcagcgattg acgacgcacc gaatgaatgg ccgcgatgat
    10381 tggtgtcaca cccatcccgc gactctatct agagctgatg tttgccctct cactgacgtc
    10441 ggtctgtcgc aattcgacag aaggcttgcg tggtgattcg gttcgcggcg tgcagcttga
    10501 cggcgctata ccctgcagac gataacccag agcgcgcatg agctcagcgt ggcggctcga
    10561 ggagctctcg aagcgccctc ggaatactgc gcggcacgtc acccccggag agaacatgag
    10621 tgagcgacac caatacctac tcgatgagag taggctcccg aaggcctggt acaacgtcaa
    10681 tgcggatctg cccgtgccgc cagcgcccgt attgcatccc cagaccttgg cgccgatcac
    10741 tccggattac ctgagcgcca tcttgcccat gaacttgatc gagcaagagg ccagtcgaga
    10801 gcgctacatc gagatccccg agccggtccg cgagatttac aagctctggc gcccgactcc
    10861 gctgatgcgc gcggtccgtt tggagaaggc tctcggcacc ccggcgcaca tctacttcaa
    10921 gtacgaaggc gtttcgccgg taggttcaca caagcccaac accgccgtag ctcaagcctt
    10981 ctacaacaag gttgcgggca ccaaggcgat tgccaccgag accggtgcgg ggcaatgggg
    11041 cgccgcgatg gccatggcct gcaacttctt cgacctcgat ctggaggtct atatggtcaa
    11101 ggtgagttac gagcagaagc cctatcgccg aatggtgatg gagaacttcg gggctcgggt
    11161 ctacgcgagc cccaccgacc gcacccacgc cggccgcgcc gtactcgcga ccgatcccac
    11221 gagcccgggc tcactcgggc tcgcgatctc cgaggcggtc gaagtggccg cgaccagcgg
    11281 gggcgcaaag aagtatgcgc tcggctcggt gctcgaacac gtgctcatgc atcagacggt
    11341 aattggggaa gaggcactgc tgcagatgga gctggcgggc gagtaccccg acgtcgtgat
    11401 cggctgcgcc ggaggcggct cgaactttgc cggcttcgtc ttcccgttcg tgcgggagaa
    11461 gctgcgcaac gggctcaaga cgcgcatcat cgccgtcgag cccacgtctt gccctacgct
    11521 cacccgcggg gtttacgctt acgattgggg cgaccaggcc aagaccgcgc ccgtggtcaa
    11581 gatgcacacg ctcggtcacg gcttcatgcc tccgggcatt cacgccggcg ggctgcgcta
    11641 tcacggcatg gccgccagcg tgagcgcgct cgtcgatcat ggggacatcg aggctcgcgc
    11701 cgtgaaccaa ctcgagacct ttgccgccgc cgtacagttc tccaaggccc aaggcatcgt
    11761 cccggccccg gagagcgcgc acgccgttcg cgccgcgatc gatgaagcgc tgaactgcaa
    11821 actgacgggc gaaccaaagg tgatcgcctt caacctctcc ggtcacggcc acttcgacat
    11881 ggccgcctac tc
//
LOCUS       DASTKU010000812        11105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1053308, whole genome shotgun sequence.
ACCESSION   DASTKU010000812 DASTKU010000000
VERSION     DASTKU010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11105
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053308"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="VFQ61_28405"
     CDS             <1..1468
                     /locus_tag="VFQ61_28405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HET9958463.1"
                     /translation="MGGWFFPHVKRSKRPGTALHHRHAGAFKSDDDTGPASLENGATP
                     ALIRAIYGFPSDLSGEGETIAFMTLGAAPRAADLAAFWAAAGIERDMPEHVRVGPPTE
                     ADEENPLYRLEATMGPAWAGAMAPKAKLVLYEIGLEVPDPWLLAVEAAVADEKRRPTV
                     LCMTWTSPEEFYYRQFNRSSISLALAKAAALGITVVCASGDWGAYDGRPSAVIRIGKN
                     QYAKIARAPWPHATFPSTEEHVLSVGGTMVSALRPLTEVAWSGPLPPDPDLQQELPFL
                     SLATSGGFSQRVPIPSFQRNALLGSGFSRVYPRGSNNPAVLPFGRGYPDVALMAAGRS
                     VARAYPRGLSATGYRLVIDDQWIDYAGGTSVAAPIWASVIANLNEARRRESRERLGFF
                     NPLLYYLARTRPLSGPHAVIRQIDDGHSDIEFRVVNGEGQVNRHVLPGYQAGAGWDPA
                     TGLGVPNVEGLVRALKSYPSSEPWIAAFDVASEVVYSE"
     gene            complement(1412..1678)
                     /locus_tag="VFQ61_28410"
     CDS             complement(1412..1678)
                     /locus_tag="VFQ61_28410"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HET9958464.1"
                     /translation="MAENEKWAQIVRRAWEEPDFKQRLITNTNEVLKEYDIQVSEGVT
                     YRIVEDELKGTRYLVLPPKLPDDGDGSLRVDDFGRDVESGDPGF"
     gene            complement(1671..2648)
                     /locus_tag="VFQ61_28415"
     CDS             complement(1671..2648)
                     /locus_tag="VFQ61_28415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958465.1"
                     /translation="MIEWLEVDAGCVVLATAGGAKLHRVQARTSDPFATPMQGRGSLP
                     TTRALLDGVVAFAAREHREPDRLESSALESSSAEVTIEGWGRRLAGYYHTTAATQRLL
                     PRILASVERAGREDLANWARHKSDAEDGHDRLALRDLTALGYDAESLVAHVVPDRARA
                     WVDLFEKLAEETVPTACLGYAHALERLALARPAHFVHQLERRLEQKLAERARSGAEAF
                     AGSQSQDRVIATRSLRVHSALGSDAGHVDENLAVIHQLCADERRTVAVACLQTARIAF
                     DRRLGREPGPNLARFHSSSPMLAGNGPVNIAEYTSKGAASTAALEHRSG"
     gene            2816..3772
                     /locus_tag="VFQ61_28420"
     CDS             2816..3772
                     /locus_tag="VFQ61_28420"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9958466.1"
                     /translation="MVVDSQPHLRTSDDGCAADPNESGQLKPADSAIKPAESAIVEDP
                     ALEPGQAKLRADVAALKRLERAALEAWPAAEVSELDGWYLRAMQGVTRRANSAWTIEA
                     RGRLTLQERIARTLEFYRARGLPVCLYVCPVSEPAGLDAELERNGFGLEGSVSVQTAT
                     PEQVLARVPGGVDAGCANGSCVEGVCALADQAGPRFRELAVHGSRYSAVPTVYEGLLD
                     RLRGRAWFAHVERNRVAVSSALAVRSGTELGLFSMLTRADSRRSGLARLLIGALAQRA
                     LEVGVRQIYLQVELGNDAAQRLYARCGFTERYQYHYRVLNRS"
     gene            3872..5833
                     /locus_tag="VFQ61_28425"
     CDS             3872..5833
                     /locus_tag="VFQ61_28425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9958467.1"
                     /translation="MTERADCVVIGGGPAGSTFAAIAAKYAPEANIILLEKEHFPRWH
                     IGESTIPVANGVLRELGVFDALDAGLGVKKLGICFVWGQDRVPWNADYLSIATDAPAG
                     TDYVIDVLGQDFSAFKSSYRPSPTPFRAFNIERSKFDHLLLNRARELGVDCREGQRVT
                     EIVRERGRVSAVRYRSEADSAIHEIQTDFVLDASGLNALATRADRERDPHMNNFAVYG
                     YFSGAEWKATYRGTREATVVFLASIPIGWIWYFPLADDVMSVGVVTHSRHFREELRRV
                     DLEQFYWEALKSCPEVFGLLKQAKLRADILPQGRRVHTSQDWSSWARQPTGPGYAAAG
                     DAAVFVDPILSSGVTLALQSGHRAAYTWLTARRQLASGAGPNEAELWQAYADYIHGEA
                     GSFLTLARFFYGNNRAAESWWWQAQRLVNASGQLDLSDRQAFTLASAGFFPVPRAIGL
                     EVVTPLVRQLTQAPASLLQIHDYPGIDRRAIGDGVKIRPLARFRLSLRAEPAPDRPGN
                     LDLRYDLATDDPAFGHRIAVLPSRISPELRPLVEAMQSPCTVGDLLAIAAQLPHGKPA
                     SEPLGSAQIETESASPAGSPKPHPPEAATTPPSVRKPQRVGANSELAQLTFELVRRAA
                     IKGFIALEFPNESPAKNPANEPASPGEFA"
     gene            6014..7135
                     /gene="trpD"
                     /locus_tag="VFQ61_28430"
     CDS             6014..7135
                     /gene="trpD"
                     /locus_tag="VFQ61_28430"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET9958468.1"
                     /translation="MTDEQKLRQFGALIVRLQQRQDLSRAEAREAYLQIWRNEQPELQ
                     QGAFISALRAKGETLEEVLGVADSHNQEWLAHCGGKVSAPEPHLGIVGVGMDSLKTTN
                     VSSGAAILAAACGLYVHKVGAPGMTGMSGCADAFALLGIDADAPVSRIHASVRESRLG
                     FSSAVGAATRSTGIFRVLSQLRCGTTVHMAGPIGFHSEDERNKIIGVPHPGQVPLVIE
                     VMRQMGYERALVPCGGSDEYVDRFMDEFSNLGTTHVAELRNGKIREFEVRPEEFGLSV
                     ARYADVAAADTREHNVQRLLRVLAGKERGPVLDLVCLNAAFCLYLMDKVSSVKEGVAR
                     AREAVAQGHALEQVRRTIRAQNADPTAGLQRLDAFLAES"
     gene            complement(7176..9665)
                     /locus_tag="VFQ61_28435"
     CDS             complement(7176..9665)
                     /locus_tag="VFQ61_28435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HET9958469.1"
                     /translation="MHRAEDTPEAPPTEVSPTGQSRAVGAEDDTVILSTEKAAPVTGI
                     RFSEGGERITALPVSSLEFEGLAARIDQKRYAILGRIGQGGMSTVYVARDESLRRQVA
                     LKLMRTDRDVHGDRFLEEMQVLGQLEHPNIVPLYTLEAAHDGTPYDTMRYLRGRSLHS
                     VLNGLRNEESQVVAEYSLTRLMQIFLQVTQAVSFAHSKGVVHRDLKPANVMLGEHGEV
                     QVVDWGIAKLAAASEVQTDSQRGLTVPGQIVGTPAYMAPEQWAGAPVEERADVYSLGV
                     MLYELLTLRRPFDETNHLAFVQALLTQPPESPRTMAPKRNIPLELEEACLKALRKVPD
                     ERQRNAKELYRQVQAWLETETDKARRHELAESKAREGRELLSAYRSQRDRVEDLERQV
                     RDLTGRFKSWQSVREKQALYYAEEDVQVARRQLVDIATDVVGVLTEAVGFERDNATAR
                     SLLADYYFDRLLEAERAAHKDEADFYARLVQRYHDGKYARQLSGEGTLTLDVFPRDPT
                     LHLCPLKEVDLRLQASEIRPLGAQPITHLALPKGSYLLLVERAGYHSARYPIYISRNR
                     DWRETVHLYREGQIQEGFCYVPGGPFIEGGDSEGWSVPRGEPHVPDFAIAKYPVTLRE
                     YLEFLNDLAERDLEQARKRAPRRAPDGGSYVGYVTGRGFVVPEELPDGLGELSAPVSG
                     VSWYDAKAYAEWRSMRDGRAYRLPTAREWEKAARGVDGRFFPWGNRFDASLCNIRESM
                     KVPRVTSVHTFETDESIYGVRGMAGNMRDWTESESKRGSDADSPSMRVVRGGAWYGGR
                     VSARCADMFWFEPTHVYFFVGFRLAHSLTRA"
     gene            complement(9814..>11105)
                     /locus_tag="VFQ61_28440"
     CDS             complement(9814..>11105)
                     /locus_tag="VFQ61_28440"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HET9958470.1"
                     /translation="ASMDHTVADTFARMAASSMLSESDSELVREIRLAQGRLTSQIRE
                     TPCTQSANLSELTQTQLFLKLENLQRTGSFKERGAGNRLALLSAAERAAGVVTASAGN
                     HAQAVALHAQKLGVHATVVMPEATPLVKIQSTRRFGAEVSLFGRNYDEAEERARTMAS
                     ERGLTYVHPFDDRFVIAGQGTLGFELIEQVPELDAVVVPVGGGGLIAGLALAIKSVRP
                     GVRIYGVESEAFPFMSQTLRQTAPERIPIGKTIADGIAVRNIGKLTRDIVQRHVEDIV
                     LVDEEEIAEAILLLLEQEKTVVEGAGAVGLAALVHRRLPVQGQAIAVILSGGNIDVSL
                     MAKIIERGLVKTGRLVRFEVYAPDVAGSLARVTDVVAQSRANIVAIHHDRAFAPNRIG
                     EALIELVLETFGFDHVQQIRGALEQAGYRAHIGQAPM"
BASE COUNT         1921 a         3628 c         3499 g         2057 t
ORIGIN      
        1 gatgggcggt tggttctttc ctcacgtgaa gcgcagtaag cgcccgggta ccgcccttca
       61 tcaccggcat gcgggcgcct tcaaatcgga cgacgacacc gggccggcga gcttggagaa
      121 cggcgccacc ccggcgttga tccgagccat ctacgggttt ccgtcggacc tgagcgggga
      181 aggcgaaacg attgctttca tgacgctcgg cgcggcaccg cgcgcggcgg atctggcggc
      241 gttttgggca gccgcgggta tcgagcgcga catgccggag cacgtccggg tggggccgcc
      301 caccgaagcg gacgaggaga acccgctgta tcggctcgag gcaaccatgg gcccggcctg
      361 ggccggggcg atggcaccca aggccaagct cgtgctctac gagattggcc tcgaggttcc
      421 cgatccctgg ctgctcgccg tggaagcggc agtcgccgac gagaagcgcc gccccaccgt
      481 gctgtgcatg acctggacca gcccggaaga gttctactac cggcagttca atcgttcctc
      541 gatttctctc gctctggcca aggctgcggc gcttggcatt accgtcgtgt gcgcctctgg
      601 ggattggggc gcgtacgatg gacgacccag cgccgtgatt cgaatcggaa agaaccagta
      661 cgcgaaaatc gcgcgtgcgc cgtggcccca cgcgacgttt ccctccaccg aggaacacgt
      721 cctctcggtg ggcggcacga tggtctccgc gctgcggccg ttgaccgagg tcgcttggtc
      781 cgggccgctc ccgcccgacc cggatttgca acaagagctg ccgttcttga gcctggccac
      841 gagcggcgga ttcagtcagc gtgtgcccat cccgagcttc cagcgcaacg ccttgctggg
      901 ctccgggttc agccgcgtct acccgcgagg cagcaataac cccgcggtgt tacctttcgg
      961 tcgtggttat ccggacgtcg ccctgatggc cgcgggacgc tcggtggccc gcgcctaccc
     1021 ccgcggtctg tcggcgacgg gctatcgtct ggtgatcgac gaccaatgga tcgattacgc
     1081 ggggggcacc agcgtggcgg cgccaatctg ggcgagcgtg atcgccaacc tgaacgaagc
     1141 gcggcgtcgc gagtcccgcg aacgcctggg cttcttcaac ccgcttcttt attatctggc
     1201 ccgcacgcgg cccctgtctg gtccgcacgc agtgattcgt cagattgacg atggtcactc
     1261 ggacatcgag tttcgcgtcg tcaacggcga gggccaagtg aatcgtcacg tgctgccggg
     1321 ctaccaagcc ggtgcgggtt gggatcccgc gaccggccta ggcgtaccga acgtggaagg
     1381 gctggtccga gcgttgaaga gctatccgag ctcagaaccc tggatcgccg ctttcgacgt
     1441 cgcgtccgaa gtcgtctact cggagtgagc cgtccccatc gtcagggagc ttggggggca
     1501 gcaccaagta acgggtgccc ttcagctcat cctcgacgat gcggtaggtt acgccttccg
     1561 acacttggat gtcgtactcc ttcagcacct cgttcgtgtt ggtgatgagg cgctgcttga
     1621 aatccggttc ttcccaagca cggcgcacga tttgtgccca tttttcgttt tcagccactg
     1681 cggtgctcca gagccgcggt agatgcggcg cccttactcg tatattccgc gatgttcacg
     1741 gggccgttgc cggcgagcat cggggatgat gaatggaatc tagccagatt cggcccaggt
     1801 tcgcggccga gccgtcgatc gaatgcaatg cgagcggtct gcaggcaagc cacggccacg
     1861 gtccggcgct cgtctgcgca tagctggtga atgactgcga gattttcgtc cacatgtcca
     1921 gcgtcgctgc caagcgcact gtgcacacgc agggagcgcg tggcaatcac ccgatcctgt
     1981 gactgagatc ccgcgaaggc ttcagcgccc gatcgggctc gttccgcgag cttctgttcg
     2041 agccgacgct cgagttggtg aacgaagtgg gcaggtcggg cgagcgccaa gcgctcgagg
     2101 gcgtgagcgt atcccaggca tgcggtcggg acggtttcct cagccagctt ctcgaacaag
     2161 tcgacccaag cgcgcgctcg gtcgggcact acgtgcgcaa ccaacgactc cgcgtcgtag
     2221 ccgagcgccg tcaggtcgcg caaagccagc cgatcgtgcc cgtcttcagc gtcggatttg
     2281 tggcgtgccc agttcgccag atcttcccgc ccggcgcgct cgacagaagc caagatacgc
     2341 ggcaacaagc gctgggtcgc ggccgtggtg tgatagtaac cggccaggcg cctgccccag
     2401 ccctcgatgg tgacctcagc gctgctgctc tcgagcgccg agctctccag ccgatcgggc
     2461 tcgcggtgtt cgcgggcggc gaacgcgacc actccgtcga gcaacgctcg cgtggtgggc
     2521 aaagagcccc ggccctgcat gggcgtagcg aatggatcgc tggtgcgagc ctgaactcga
     2581 tgcagcttcg cgccgcctgc cgttgcaagc acgacacagc ctgcgtcgac ttcgagccac
     2641 tcgatcattt cgggttttcg cgcggtttcc cgccggagta agggcgcagc cgttcctttc
     2701 ggcaggctgc cggacgcggt accgcccgat cggaccagct acgcacccga gtagaacaca
     2761 ggaagtggtg ctcggggaag agtcacaggc gcgcaatgta aaaagtgata ctgccgtggt
     2821 cgtggactcc cagccccacc tccgcactag cgacgatggc tgcgcagctg acccgaacga
     2881 gtcaggtcag ctgaaaccag cagactcagc cataaaacca gcagagtcag ccatagtcga
     2941 ggatcccgcg ctcgagccgg ggcaggcaaa attgcgtgca gacgtggccg ctttgaaacg
     3001 cctcgagcga gcggcactcg aggcatggcc cgcggccgag gtgagcgaac tcgacggctg
     3061 gtacttgcgg gctatgcagg gcgttacccg tcgagccaat tcggcttgga cgatcgaggc
     3121 acgggggcgg ctcacgttac aagagcggat cgcgcgcacc cttgagttct atcgagcgag
     3181 ggggcttccc gtatgtctct acgtttgccc ggtgagcgag ccagcgggcc tcgatgcgga
     3241 actcgagcgt aacggtttcg ggctggaggg ttcggtgtcc gtgcaaaccg caacgcctga
     3301 gcaggtgctc gcgcgcgtgc cgggtggcgt cgatgccggc tgcgcgaatg gcagctgtgt
     3361 cgagggcgtc tgtgcgctcg ccgaccaggc cggtccgcgt tttcgtgagc tggccgtgca
     3421 cggctcgcgc tactcggccg tgcccacggt ttatgagggt ttgttggatc ggctgcgcgg
     3481 acgcgcatgg tttgcccacg tggaacggaa tcgggttgcc gtttcttcag cgctcgccgt
     3541 acggagcggc acggagctcg gcctattctc gatgcttact cgggccgact cacggcgcag
     3601 cggcctcgct cgactcttga tcggtgcctt ggcgcaacgc gcgctcgagg ttggggtgcg
     3661 gcaaatctat ctccaggtcg aactggggaa cgacgccgct cagcgtctat acgcgaggtg
     3721 cggtttcacg gaacgttacc aataccacta tcgcgtgctg aacaggtcat gagctacgcc
     3781 gctcatgacc tcggggccac cggttccgac gcgtgctgcg ccccaaattg ttgtcgttat
     3841 ctcgcctgcg ctgcactagg gagtgaactc gatgacggaa cgcgcagact gtgtcgtaat
     3901 cggcggggga ccggcaggta gcaccttcgc ggcgatcgct gccaagtacg cccccgaggc
     3961 gaacatcatt ctgctcgaga aggagcactt tccgcgctgg cacatcggcg agtccaccat
     4021 tcccgtggcc aatggcgtgc tgcgtgaact cggcgtgttc gacgctctcg atgcgggtct
     4081 gggggtgaag aagctcggca tctgcttcgt ctggggtcag gaccgtgtgc cttggaatgc
     4141 ggactatctg agcatcgcga ccgatgcgcc tgcgggcacg gactacgtca tcgacgtgct
     4201 cgggcaggat ttcagcgcgt tcaagagctc gtaccggcca agcccaactc cgtttcgcgc
     4261 cttcaacatc gaacgctcca aattcgatca tttgttgttg aatcgtgcgc gcgagctggg
     4321 ggtcgactgc cgtgagggtc agcgggtgac ggaaatcgtg cgcgagcgcg ggcgtgtctc
     4381 ggcggtgcgc tatcgctccg aagccgactc ggcgatccac gagatccaga ccgatttcgt
     4441 gctcgacgcg agcggtttga atgcgctcgc cacgcgtgcg gatcgtgaac gcgatccgca
     4501 catgaacaac ttcgcggttt atggttactt ctcgggggcc gagtggaagg ctacctatcg
     4561 cggcacgcgc gaggcgacgg tcgttttcct ggccagcatc ccgatcggtt ggatttggta
     4621 tttcccactc gcggacgacg tgatgagcgt cggtgtggtc actcacagcc gccacttccg
     4681 cgaggagcta cgccgagtgg atctggagca gttctattgg gaggccttga agagctgtcc
     4741 ggaggtgttc gggctgctga agcaggccaa gttacgcgcc gatattctgc cgcagggcag
     4801 gcgtgtccac acgagtcaag attggtcgtc ttgggcacgg cagccgaccg ggcctgggta
     4861 cgcggccgcg ggcgacgcgg cggtgttcgt ggatccgatt ctatcgtccg gagtgactct
     4921 cgccctccag agcgggcatc gggcggcgta tacgtggctg acggcgcggc gtcagctcgc
     4981 gagcggagcg ggcccgaatg aagccgagct gtggcaagcg tatgccgatt acatccacgg
     5041 tgaggccggc agcttcctga cgctggctcg cttcttttac ggcaacaacc gagccgcgga
     5101 gtcttggtgg tggcaggcgc aacgcctagt gaacgctagc ggccagctcg atctgagtga
     5161 tcgccaggcg tttacgttgg cgagcgcggg ttttttcccc gtgccgcgcg ccatcgggtt
     5221 ggaggtcgtg acgccgctcg tccgacaact cacccaagcc cccgcctccc tcctgcagat
     5281 ccacgactat cccgggatag atcgacgggc gattggggac ggggtgaaaa tacggccttt
     5341 ggcgaggttt aggctctcac tgcgcgcgga gcccgcgccc gaccgacccg gtaacttgga
     5401 cctccgctac gacttagcta cggacgaccc tgctttcggc caccgcatcg cggtccttcc
     5461 gagccggatt tcgccggagt tgcgaccgct cgtcgaagcc atgcagagcc cgtgcacggt
     5521 aggagatttg ttggcgatag cggcgcagct accgcacggc aaacccgctt cggaaccgct
     5581 cggctcagcg caaatcgaga cagagagcgc gtcacctgca ggttcgccca aacctcatcc
     5641 tcccgaagct gcgacaaccc cgccgagtgt gcggaaacct cagcgtgtcg gagctaactc
     5701 cgaactagca cagctaacct tcgagttagt gcggcgtgcc gccatcaaag gcttcatcgc
     5761 gctcgaattc cccaacgaaa gtcccgcgaa gaaccccgcg aacgagccgg catcgcccgg
     5821 tgaattcgcg tgagtcctca gcccatcagt cgtcgctgcc gtgagcctaa ccggcagcac
     5881 tcaccagctc tgcgcacgag cagcgcgggt ggtggccagg tgagaaggaa acgcgccgac
     5941 acggccgttc aaggtcctag caacccggtc gacgagtgtg ctctactttg catcgacaaa
     6001 ggcagggaca cgcatgacgg acgagcaaaa actccggcag ttcggcgcac tcatcgtgag
     6061 gcttcagcaa cgccaagatt tatcgcgcgc ggaggctcgc gaagcgtacc tccagatttg
     6121 gcgcaacgag cagcccgagc tccagcaagg cgcgttcatc tccgccttgc gcgccaaagg
     6181 cgaaacactc gaagaagtgt tgggcgtcgc cgatagtcac aatcaggagt ggctcgcgca
     6241 ctgcggcggt aaagtctccg cccccgagcc gcacctcggc atcgtgggcg tgggtatgga
     6301 tagcctgaag accaccaacg tctctagcgg cgccgcgatc ttagccgcag cttgcgggct
     6361 ctacgtgcac aaggtcgggg cacccggcat gaccggaatg agcggttgcg cggacgcgtt
     6421 cgctctgctc ggcatcgacg cagacgcgcc cgtatcacgc attcatgcgt ccgtgcgcga
     6481 gtcgcggctc ggcttctcct cggccgtcgg cgcggccacg cgatcgacgg ggatcttccg
     6541 ggtgttgagc cagctgcgct gtggaaccac ggtgcacatg gcaggcccga ttggtttcca
     6601 cagcgaggac gaacgcaaca agatcatcgg cgtgccgcac cccggtcaag tcccgctggt
     6661 catcgaagtc atgcggcaaa tgggctacga gcgcgcgctc gtgccgtgcg gcggttccga
     6721 tgaatacgtg gatcgcttca tggacgagtt cagcaatctg ggcaccacgc acgttgcgga
     6781 gctgcgcaac ggcaagattc gtgagttcga agtgcggccg gaggagtttg ggctatccgt
     6841 ggcgcgctac gcggacgtgg cggccgccga tacgcgcgag cacaatgtgc agcgtttgct
     6901 gcgggtgctg gcgggcaaag aacgcggccc agtgctcgac ctagtctgct tgaacgccgc
     6961 cttctgtctt tacctcatgg acaaggtatc gagtgtcaaa gagggcgtgg cgcgggcccg
     7021 agaagccgtg gcccaagggc acgccctcga gcaggtgcgg cgtaccattc gcgctcagaa
     7081 cgccgatccc accgcgggtt tgcagcggct ggatgcgttt ttggccgagt cctaggcttc
     7141 cgcccgaggg cacacgttgt cggagcaggg ctcgctcagg cccgcgtcag cgaatgagcg
     7201 agccggaagc ccacgaagaa gtagacgtgg gtgggttcga accaaaacat gtcggcgcag
     7261 cgggcgctca cgcgcccgcc gtaccacgcg cccccgcgaa ccacgcgcat ggaagggcta
     7321 tctgcgtcgc tgccgcgctt ggactcgctc tccgtccaat cccgcatgtt gccggccatg
     7381 cctctcaccc cgtaaatcga ctcgtccgtc tcgaacgtgt ggacgctcgt cacgcgcggc
     7441 accttcatcg attcgcggat gttgcaaagg ctggcgtcga accgattgcc ccacgggaaa
     7501 aaacgcccat ccacgccccg tgccgccttc tcccattctc gtgcggtcgg caatcggtag
     7561 gcgcggccat cacgcattga acgccactcg gcatacgctt tggcgtcata ccagctcacg
     7621 ccggacacgg gtgccgacag ctcgcccaag ccgtcgggca actcctccgg caccacgaaa
     7681 ccgcgcccgg tgacgtaccc gacgtagctg cctccgtcgg gtgcccgtcg gggcgcccgc
     7741 ttgcgcgcct gctcgagatc ccgttcggcc aggtcattca aaaactccag atactcgcgc
     7801 aaggtcaccg gatactttgc gatcgcgaaa tccggcacgt gaggttcgcc gcggggcaca
     7861 ctccagccct cgctgtcgcc gccctcgatg aagggcccac ccggcacata gcaaaatccc
     7921 tcctggattt gcccttcccg atacaagtgc accgtctcgc gccagtcgcg attacgcgag
     7981 atgtagattg gatatcgagc actgtggtag cccgcgcgct cgaccaacag cagatagcta
     8041 cctttgggta aggctagatg cgtgatcggc tgcgccccga ggggacggat ctcgctagct
     8101 tgcagccgca gatccacctc tttcaatggg cacaaatgca gcgtcggatc acgcgggaac
     8161 acgtccaagg tcagcgtgcc ctctccggac agttgccgcg catacttgcc gtcgtgatag
     8221 cgctgcacca ggcgcgcata gaaatcggct tcgtccttgt gcgccgcccg ttccgcctcg
     8281 agcaagcggt cgaagtaata gtccgcgagc agcgaacggg ccgtcgcgtt gtcgcgctcg
     8341 aaccccaccg cctccgtcaa caccccgacc acgtccgtcg caatgtccac gagttgccgt
     8401 cgcgcgacct gcacatcttc ttcggcgtag tacagcgcct gcttctcgcg cacgctctgc
     8461 cagctcttga agcgacccgt cagatcgcgc acctggcgct ccaaatcttc cacgcgatcg
     8521 cgttgcgagc gatacgcgga gagcagctcg cgcccttcgc gcgccttgga ctcggcgagc
     8581 tcgtgccgcc gcgctttgtc cgtctccgtt tccaaccagg cctggacttg gcggtacagt
     8641 tccttggcgt tgcgttgccg ctcgtccggc accttgcgca acgccttcag acacgcctcc
     8701 tcgagctcga gcgggatgtt gcgtttgggc gccatcgtgc gcggcgactc tgggggctgc
     8761 gtgagcaagg cctgcacgaa cgcgaggtga ttggtctcgt cgaacggcct gcgcagcgtg
     8821 agcagctcgt acagcattac gcccagcgaa tacacgtctg cgcgttcttc cacgggagca
     8881 ccggcccatt gctctggcgc catgtaggca ggcgttccca cgatttgacc gggcacggtc
     8941 agaccgcgct gcgagtccgt ctgcacctcg ctagccgcgg cgagcttggc gataccccaa
     9001 tccacgactt gtacttcccc gtgctcgccc agcatcacgt tcgcgggctt caaatcgcga
     9061 tgcaccacgc ccttgctgtg cgcgaacgag accgcctgcg tgacctgcaa gaaaatctgc
     9121 atcaagcgcg tcagcgagta ctcggcgacg acctgggact cctcgtttcg cagcccgttc
     9181 agcacgctgt gcaacgagcg cccacgcaga tagcgcatgg tgtcgtacgg cgttccatcg
     9241 tgcgcggcct ccaacgtgta gagcggcacg atgttcgggt gttcgagctg accgagcacc
     9301 tgcatttctt cgagaaagcg gtccccgtgc acgtcacggt ccgtgcgcat caacttcagc
     9361 gcaacctgac gtcgcaacga ctcgtctcgc gcgacgtaca ccgtcgacat cccgccctgg
     9421 ccgatgcggc cgaggatggc gtaacgcttc tgatcgatgc gggcggccaa gccctcgaac
     9481 tcgaggctcg acaccggcag cgcggtgatg cgctcgccgc cctcggagaa ccgaatgccc
     9541 gtgaccgggg cggccttttc cgtgctcagg atgaccgtgt cgtcttccgc acccacggct
     9601 cgggattggc ccgttggcga aacctcggtg ggcggggctt ccggagtatc ttcggctcgg
     9661 tgcatcgggc agatctcgcg ctcaagcatt cctcaaacaa aaccctgcgt ccaaccaatc
     9721 tgtgggccca attggccggc gctctcgagg ctttgctggc tgcacccttc tcgcgagcgc
     9781 ttggcacgca cgggccgttt accccggtct tgcctacatt ggcgcttgcc cgatgtgagc
     9841 tcggtaaccg gcctgctcca gcgctccacg gatctgctgc acgtgatcga agccgaacgt
     9901 ctcgagcacc agttcaatca gggcctcgcc aatccgattg ggagcgaacg cacggtcgtg
     9961 atgaatggcg acgatgttgg cgcggctttg cgccacgacg tccgtcaccc gagctaacga
    10021 tccggctaca tccggcgcat agacctcgaa tcgcaccagg cgaccggtct tgaccaaacc
    10081 tcgctcgatg atcttggcca tcaaactcac gtcgatgttc cctccgctca ggatcacggc
    10141 aatggcttgc ccctgaaccg gcagccggcg atgcaccagc gccgcgagtc cgaccgcacc
    10201 cgcgccctcc accacggttt tttcttgctc gagcagcagc aaaattgctt ccgcgatttc
    10261 ttcttcgtcg acgagcacga tgtcttctac gtgccgctgg acgatatcgc gcgtgagctt
    10321 accgatgttc cgcaccgcga tgccgtccgc gatggtcttg ccgattggga tccgttctgg
    10381 ggcggtttgc cgcagagtct gactcatgaa tgggaaggct tctgactcca cgccgtaaat
    10441 ccgcacgccc gggcgtacgg acttgatcgc caacgccaag cccgcgatta atccgccacc
    10501 tcccaccgga accaccaccg cgtcgagctc gggcacttgc tcgatcagct cgaaccctag
    10561 cgtgccttgc cccgcgatca cgaagcggtc gtcgaacgga tgaacgtacg tcagccctcg
    10621 ttcgctggcc atggttctag cccgttcctc ggcctcgtcg tagttgcgcc cgaacaacga
    10681 cacctcggct ccgaagcgcc gagtggattg gattttgacg aggggcgtcg cctccggcat
    10741 gaccaccgtc gcgtgaaccc cgagcttttg agcatggagc gcaaccgcct gcgcgtgatt
    10801 gcccgcgctc gcggtcacca cacccgccgc gcgctcggct gcactcagta gtgccagacg
    10861 attgcccgcg ccgcgctcct tgaacgagcc ggtacgttgc agattctcga gcttcaaaaa
    10921 cagctgtgtt tgcgtgagct ccgatagatt cgcgctctgc gtgcacggcg tttcgcggat
    10981 ctgagacgtg agccggccct gagccaaccg aatctcccga accaactccg aatccgattc
    11041 gctgagcatt gacgacgccg ccattctggc gaatgtatca gccaccgtgt ggtccatcga
    11101 agccc
//
LOCUS       DASTKU010000813         3309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1501866, whole genome shotgun sequence.
ACCESSION   DASTKU010000813 DASTKU010000000
VERSION     DASTKU010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501866"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1181..1471
                     /locus_tag="VFQ61_28445"
     CDS             1181..1471
                     /locus_tag="VFQ61_28445"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HET9958471.1"
                     /translation="MELRVLADQIEIYAVKHNEFPPELGWYEALKGDGLVSAGGERDP
                     WGNAYVYNRKGGDFDLRTVGMDGKFATADDQVRAQGWRWTTCAEPRFWRCNK"
     gene            2002..3156
                     /locus_tag="VFQ61_28450"
     CDS             2002..3156
                     /locus_tag="VFQ61_28450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958472.1"
                     /translation="MAACSQVTAAKPPSQDTVPRQPAQPSSSAAASSRALPLSPEAQP
                     ASQASSPPDCIELSSPAEPSTASDTGNCSVRSARASLASVRVTVSPRESFQLRLSDVA
                     LELTPSMSPMGPVRVSARAPLAFEGTTVPPRLKLAPGTALGEGLVKAGKDVRLVKYSR
                     VGDKVTGELEIGPYRIGPLQAPCSALRFDESSYSRADPLPPRHPLESNRTTLTLHPTV
                     RGNELSGTPVQISESPVLNRTRQQGLLLELSLSFEDGSRLSGWAEASALHPVADGRRG
                     GGVSEGLSLCSPGCPDGRVGRGVDGPVKYAGRARLKAQTSIHAAPDGIPWATAPTELE
                     VELTQSERSAWVRVDRIPGIEEDSGCHALLHAFVHEANISYTKRPGGNSQ"
BASE COUNT          653 a          976 c         1078 g          602 t
ORIGIN      
        1 gatggtactg agtctcgagt gcgtccgcca gacctgctat ccagcccgac gacgtggggc
       61 tgtggaacac ccgcgtggcc gctatcgtga gggtgatggc atcaaaacgg gcggatcgca
      121 gccgttgacg caagtcaggc aacgaccgtc gcgtgagcaa ccatcatcca gaaccactcc
      181 cgatgcgcaa tcccgcaccg cgcagattgg cgtagtccga cgcagggagt ggctgggttt
      241 atggttatcc gtcgcgcagc ggctacaacc ggcggatatc caaagaacgg aaaagacggc
      301 ccaacacccg acgaaaccgg gagcaaacct ccgtccgcgg cgcggaggtg gtcggcaact
      361 atctgttgga tccgcggcgg gcggtgaagt ggctcgggcc tcttttgagc gtttaagggt
      421 gaagattcgg ccggtcgccc acccacgcac gttcggcccg gcgcctccgg cacttgcgcc
      481 cgccactaca gcgccgcctt caaacgcgca gccctgcgtg gtgcgcccga acaccggatg
      541 ggtctgcacc tcgaagtaac cggaaggtta cgcactaacc cggcgtaagc gtacgttggt
      601 acctgtacgt gggaggtttt acggctgact tgatggcaag aatggggttt ggtgagttcg
      661 tcgcgagggc ggagagggag gggatgccca cacgcttcct gcgagaggtc agcctcagta
      721 cctatacttt cgattacggg gaagaggacg aggtgggccc atacttgaac gtcatgactc
      781 ttcgtagaga aaccctgcaa agcgcggtca atctctcgct ggaaaacctc tggagcgagg
      841 cttcggctgt gcaaacaatg catcacgagg ccacacatgc gtggtttgac ataatgtcgg
      901 aacgtccgga tgtcgcctcg ctcatcgcgc acggtgagac atactatatg aacgctccac
      961 ttgagaatgg cgcagccgcc agcggccggc tgctacgtag gccagcgtgc agcgacgttt
     1021 tggcgggctc tgaagaagct aacgcgcctt cagcaggcgt gccaggcgtc aaaggatctc
     1081 cccaatgcga aacacagttt gggcgcgcgc tgttgtggcg gtcgtcctgg gtgcaactcc
     1141 aggagtgaga gcccatattc cgccctgtga tagggcgcgg atggagctcc gggtcctggc
     1201 tgatcaaata gagatatatg cagtgaagca caacgaattc ccgccggagc tgggctggta
     1261 cgaagcgttg aaaggggacg gccttgtctc agccggagga gagcgcgatc catggggcaa
     1321 cgcctacgtt tacaatagga agggtggcga tttcgatctg cgcacagttg gcatggatgg
     1381 caagttcgca acggcagatg accaggtgcg cgcgcaaggc tggcgctgga ccacgtgcgc
     1441 tgaaccgcga ttctggcgtt gcaacaagta gcacgatcgc aaggagcgac tacgctacgc
     1501 ggagcgcgtc tgctcttgga actccttgga cggatcgagt ttccgctcca gaagaggcca
     1561 ttcattctca gttccgtatg gtctggcggt ctcatcgcga cgcagggtgc cttgctgtct
     1621 ggccctctgt agagaaaccc tgcaaagcgc ggtcaacatc tcgctagaaa cctctggcgc
     1681 gaggcttcga ctgttcaaac aatgtatcac gaggccgggc gaggtgaggg cggggcgacc
     1741 gaagcggatg tccgcggggt ggctctggtg gcgctgccga aagcggagct ggtgctcgtc
     1801 ggatagtcac ggctgaggcg ttgcaacgaa cccgtgtgat cccggtccca tggcagagag
     1861 aggcacttga ctcggagacg gagaatgcgt gagcatagcg agcgtatggg tgatttagca
     1921 ggaagcggga gggggacgcg cgctccgagc agcaaccgtc gaccttgcgt ctcgggggcc
     1981 aggctggtcc ttggattgtc gatggcggcc tgctcccaag ttaccgcagc aaagccgccg
     2041 agccaggaca ccgttccacg tcagcccgca cagccgagtt cgagtgcagc ggcgagttcg
     2101 agggcgctgc cgctctcgcc agaggctcaa ccggcatcgc aggcgtcgag tcctcccgac
     2161 tgtatagagc tcagctcacc cgcggagccc tctacggcaa gtgatacggg caactgcagc
     2221 gttcgctcgg cgcgcgctag cctggcgagc gttcgcgtca ccgtgtctcc ccgtgaatcc
     2281 ttccaacttc gattgtcgga tgtagcgctc gaattgacgc ccagcatgag ccctatgggg
     2341 ccggttcgag tctctgcgcg cgcgccgctg gccttcgaag gcactaccgt acctccgcgg
     2401 ctcaaacttg cccccggcac cgcgctcggt gagggtttgg tgaaggcggg caaggacgtg
     2461 cgtctcgtca agtactctcg agtgggcgac aaggtgacgg gtgagctcga gatcggcccc
     2521 taccgcatcg gtccgctgca agccccctgc tccgcgctgc gcttcgacga atcgagctac
     2581 tctcgagccg atccgctgcc tccgcgtcat ccgctggaat cgaatcggac aacgttgacg
     2641 ctgcacccta cggtccgggg aaacgagctg tccggtactc ccgtgcagat tagcgagagc
     2701 ccagtcttga accgcactcg tcaacaggga ctcttactcg aactcagcct gtccttcgag
     2761 gacgggagca ggctctccgg ctgggcggaa gcttcagcgt tgcatcccgt agctgacggc
     2821 cggagagggg gaggcgtgag cgaaggtcta tcgctctgct cacccggctg ccctgacggt
     2881 cgcgtcggga gaggtgtgga cgggccagta aagtacgcag gccgcgctcg actcaaggca
     2941 caaacctcca ttcacgccgc tcctgatggc attccttggg cgaccgcgcc cacggaactc
     3001 gaagtcgaac tgacgcaatc ggagcgtagc gcctgggtgc gcgtcgatcg aataccgggc
     3061 atcgaggaag attcgggctg tcacgctttg ctgcacgcct tcgtgcacga ggcgaatatc
     3121 tcgtacacaa agcgaccagg gggaaactct caatgaccag cgctgcgatg cccgtttgca
     3181 gcgaacttga ccacccattt gcgctgaagt tgaccacctg cgcagttcgg gattcggccg
     3241 agcggagcgt gagcgacgcg ggtgcctaaa ttctgaaact gaggttagaa gttgtgggcc
     3301 acgagggcc
//
LOCUS       DASTKU010000814         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_606708, whole genome shotgun sequence.
ACCESSION   DASTKU010000814 DASTKU010000000
VERSION     DASTKU010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606708"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VFQ61_28455"
     CDS             <1..638
                     /locus_tag="VFQ61_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958473.1"
                     /translation="SLSPAELARFASLADRQPSNPALVSELRDPGLAYGEERDIPLSR
                     RQMLWLKYRYGVYFPWKVYFEFIPNRYWSDKNRSEGKDFTRLARAVFPKTIAFVKSLP
                     FSSIGRCNLMGLEANDHGTVHRDGERTDPEPDHFITICPAGNKTLFLWDELSQRKVPV
                     RGRAYWFNDNDYHGVEAAPFFRYSLRIDGTFEPGFLARLRSRYSKESGAFA"
     gene            635..1987
                     /locus_tag="VFQ61_28460"
     CDS             635..1987
                     /locus_tag="VFQ61_28460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958474.1"
                     /translation="MSDGGPFERDAVVNEPGLIGARWWQKSLTDTHARENGVTRRSAI
                     TVALGATGALVGFAVVVGLLSRSCDDEDLDIDRDKALNLQKKYGWSFGAAQESVTFDG
                     SSTRPFDRSALARLALDARPRVPALEPYYVPALFESPSARPSASAEGDPEVIVPLDQV
                     LLPIYTPQMARAFRQGKALASLFRELGVLSGAIVVVDLDGPESVAFAAGASSDLEPVF
                     SFGNWPHPRGVVPAHRTLAAAAYFQPLFAGRQAAATRTPLFVLDRQRLAAYSDDASQF
                     DNRYLAELPTAAALLNWGVSRVLYVAPQDGPTSELADLNELFVDYDAKAIEVKLVTAG
                     SFAPDPTDPSPESDPIPPSGSDSSHFYYGGGRASHAWFWHDYPWARGPLPQAVEPSMF
                     RPGPYYRPSLSRAAFSHSNPPPQFGTVPIMVHPSTRRVMGAAMSRSGSWNRTYGSSSS
                     "
     gene            2033..>2517
                     /locus_tag="VFQ61_28465"
     CDS             2033..>2517
                     /locus_tag="VFQ61_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958475.1"
                     /translation="MDPQAALHRALLEAVPAQNTTLSAGQKWHQYQRAASLLLNNLHL
                     AERGCWDYFNDDERAQRDFEQWTRGMTTREGVRPGPRALAPVQYEARYLTFTMAFLIE
                     QDSWSDLALRGYCNVPESKLWQRDTFGHILRNFGWVSFACVKADVAYLIPRDLEWALT
                     A"
BASE COUNT          434 a          805 c          779 g          499 t
ORIGIN      
        1 gcagcctttc ccccgccgaa ctagcccgct ttgcctcgct agcggaccgg cagccgtcca
       61 acccagcact cgtttccgag ttgcgtgacc ctggtctagc ttacggtgag gagcgagata
      121 tcccgctttc tcgccggcag atgctctggc tgaagtatcg ctacggcgtg tacttcccgt
      181 ggaaggtgta cttcgagttc attccgaatc ggtactggag tgacaagaac cgaagtgagg
      241 gcaaggattt cacacgcctg gcccgcgccg tgtttcccaa gaccatcgca ttcgtgaaga
      301 gcctcccgtt ttccagtatt ggtcgctgca acttgatggg gctcgaggcc aacgaccacg
      361 gcacggttca ccgcgatggg gaacgaaccg acccggagcc agatcatttc attacgatct
      421 gcccggcggg caataagacg ctcttcttat gggatgagct cagccaacga aaggtgcccg
      481 tgcgcggccg cgcctattgg ttcaatgata acgactacca tggtgtcgaa gccgcgccgt
      541 tcttccggta ctccctgcgc atcgacggca cgttcgagcc tggcttcttg gcgcggctgc
      601 gctcccggta ttcgaaagag tcaggagcgt tcgcgtgagt gacggcggtc ccttcgagcg
      661 cgacgcggtc gtgaacgagc caggcctgat cggggcacgc tggtggcaga agagtctaac
      721 cgacacccac gctcgagaga acggggtgac tcggcggagt gccatcactg tggcgcttgg
      781 cgcgaccggt gcactcgtgg gttttgccgt ggtggtgggc ctgctctccc gaagctgtga
      841 cgatgaggat ttggatatcg accgcgacaa ggcgctcaat ctccaaaaga agtacggctg
      901 gagtttcggg gccgctcagg agagcgtcac cttcgacggc agctcgaccc gcccgttcga
      961 tcgcagcgcg ttggctcgtc tggcgctcga cgcccggcct cgagttcccg cgctcgagcc
     1021 gtactacgtg ccggccctgt tcgaatctcc gagcgcacgc ccctccgctt cggccgaggg
     1081 tgacccagag gtgatcgtgc cgctcgatca agtcttgctc cccatctaca ctccgcagat
     1141 ggcgcgcgca tttcgccagg gcaaggcgct cgccagtttg tttcgcgaat tgggagtgct
     1201 ctcgggcgcg atcgtcgtcg tcgacttgga cggtccggaa tcggtcgcgt tcgcggccgg
     1261 cgctagtagc gatctcgagc cggtattcag cttcggtaat tggccgcacc cccgcggcgt
     1321 ggtgcccgcg catcgtacgt tggccgcggc cgcatacttt caaccgctgt tcgcgggtcg
     1381 acaggcggcc gccactagaa cgccgctctt cgtgctcgat cgacagcggc tcgccgcgta
     1441 ctcggacgac gcgagtcagt tcgacaatcg atatctggct gaactcccta ccgcggcggc
     1501 cttgctcaac tggggagttt ctcgagtgct gtacgtcgct ccgcaagatg gcccaacctc
     1561 cgaactcgcc gatctgaacg agctctttgt ggactacgac gcgaaagcca tcgaagtgaa
     1621 gctcgtcacg gcgggtagct ttgctccgga tcccactgat cccagcccgg aatcagaccc
     1681 gattcctccg tcgggctcag attcgagcca cttctactac ggtggcgggc gggcatcgca
     1741 tgcctggttc tggcacgatt atccctgggc gcgcgggccg ttgccgcagg cagtggagcc
     1801 gtccatgttc cggccggggc cttactacag gccgagcctc tcgcgagcgg cgttctctca
     1861 ttccaaccct ccaccccagt ttggcaccgt gcccatcatg gtgcacccca gcacccgacg
     1921 agtgatgggc gccgcaatga gccgaagcgg atcttggaac cgcacttacg ggtcgagcag
     1981 cagctgagcg ggcatggaat actcgatatt tgcaatggag atctgcgctc gaatggaccc
     2041 ccaggcagcg ctgcaccgcg ccctgctcga ggccgttccg gcccagaata ccacgctgag
     2101 cgcgggtcag aagtggcacc aataccagcg tgccgcgtcg ctgttgctca acaatctcca
     2161 tttggcggag cgcgggtgct gggactactt caacgacgac gagcgcgcgc aacgcgactt
     2221 cgagcaatgg acgcggggca tgacgacgcg cgaaggagtg cggcctggtc ctcgcgcgct
     2281 cgctcccgtc cagtacgagg cgcgctatct gaccttcacg atggcctttt tgatcgagca
     2341 agattcgtgg tcggacctgg ccctccgtgg ctactgcaac gtccctgaat ccaagctatg
     2401 gcagcgggat accttcggcc atattttgcg caacttcggc tgggtgagct tcgcctgtgt
     2461 caaagcggac gtggcctatc tcatcccgag ggatctcgag tgggcgctga ccgcgga
//
LOCUS       DASTKU010000815        11567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_606820, whole genome shotgun sequence.
ACCESSION   DASTKU010000815 DASTKU010000000
VERSION     DASTKU010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11567
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606820"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..568
                     /locus_tag="VFQ61_28470"
     CDS             179..568
                     /locus_tag="VFQ61_28470"
                     /inference="COORDINATES: protein motif:HMM:NF026923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm7 family immunity protein"
                     /protein_id="HET9958476.1"
                     /translation="MYSYHLWIVLAESTQENDIGGLQDKINGLRTLLAESLDTRPEHP
                     IIKVNCDWLFQCSVSHNHKGDAQERLESVLRWITRRLPGSYGLVYYRDDEDPREANSY
                     RVLVVSRGGVVERTDPFLSPVVPTIED"
     gene            673..2313
                     /locus_tag="VFQ61_28475"
     CDS             673..2313
                     /locus_tag="VFQ61_28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HET9958477.1"
                     /translation="MKYASHFSTLQTPQTEKAAPNQVQNNAGGFSFQITPQQQLERWL
                     VLGSEGGTYYVTEKKLTRDNAKTVIACLDADGPGTVSMIASFSESGRAPKNDPAIFAL
                     AIASGHSDSATRKAALDALPRVCRTGTHLFQFASVVKNFRGWGRGLRSAVGRWYSEKS
                     PDQLAYQIAKYQQREGWSHRDLMRLAHPGPKHAAIYRYIVTGAGALGDREVKRGERVS
                     SYAGVGELPPFLAAVEEAKQADEHRTILLIREHGLTHEMVNSEHLRSPDVWEALLERM
                     PLTAMLRSLARMTNIGLLKPLSNAATKVGDTVSNEARLKKARVHPIQVLSALMTYKQG
                     HGMRGSLTWSPAPQIVDALDAGFYAAFGAIEPSGARTLLGIDVSGSMGGSKLVGIDGL
                     TARHGAAVMAMVTARVEKQWHILGFSDRLVDLPITPRQRLDDVISTMDRLPFGRTDCA
                     QPMLHAAANGLNVDVFHVYTDNETWCGNIHPHQALGAYRAKNGINSKLAVIAFSATNF
                     SIADPSDARELDFVGFDAAAPQVLADFGRERSLVAFEG"
     gene            2499..2633
                     /locus_tag="VFQ61_28480"
     tRNA            2499..2633
                     /locus_tag="VFQ61_28480"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            2981..5698
                     /locus_tag="VFQ61_28485"
     CDS             2981..5698
                     /locus_tag="VFQ61_28485"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET9958478.1"
                     /translation="MTSLPMDPRVQTGRLPNGLTYYLQRHQPEQRRAHVYFVVKAGSV
                     QEEEDQRGLAHFVEHMAFNGTRRFAKQELVELLEKSGLTFGADMNAGTTYDHTRYHLS
                     VPTDVPHLLGTALDVLEDWAQAITFMADEVEKERSVILSEWTSSQGAQRRLSEQRLAL
                     ILAGSRFADRYALGNRRVLEGASPERIRDYYRRWYRPERVAVVVVGDIEPQALKNEIE
                     RRFAGWSSPDPLSDPNSNIPVRHTTSLTVLTDSETPAAIVRVLLKAPARLVRSVEDLK
                     DQTQSQLGAAMLRHRLEALERSAEASFTDSSCSLTPSVLGRLDLFEVLAMAKQGRVQD
                     TLERLVMELERVRRHGFTDDELARAKAERLRFFEHALAAQNTFASTQVAEPLIKHFLT
                     GDVTPSLEQRVQFERSFVADIVAADVSRRMSRWLTESEELVIASGPERDALPSQEQLN
                     AALVAAKSRSVEAYRDEIPNIPLMPTLPQPGRIVKEDHIAEIGVSVWTLSNGARVVLK
                     PTDFKADEILGQAISFGGTATLPAREFGDARLAHEIVTTSGVGALDRQQLARALAGKA
                     VTVYPWIAEQDEGIRANAAPQDLETMFQLVHLYATAPRRDEAAFEVYRAQLVNQLRNR
                     DLHVPSAFTDAIGNALWGGDARRRVPSLGEVKAVDLNRALEFYKQRFSDVSDFSFVFV
                     GKIDLTTFRPLVERYLASLPGGSRHETSREIGLHRKSGITKVRIKKGKEDAAALSLLY
                     HGESPWSENAHTDLVSLQSYLAIRLREVLREELGAVYTPYVNANFERVPYDAYSLTIS
                     LDCKPSDVEKVLRATRQIIDDTKKTGAAAGYVDKIRKQRTRDLDRMYHENGFWLERLV
                     DKYKLREDPRNILILHDLTQRVTSENIKQAARHFLREDQYLEAELQPER"
     gene            5751..6515
                     /locus_tag="VFQ61_28490"
     CDS             5751..6515
                     /locus_tag="VFQ61_28490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958479.1"
                     /translation="MVDRSTSRRGQQNHLDDGRKAGDSDWLDGLGMGMKYGTRLASTR
                     DARSSGLAAMAFLLASISLHCEPRSSPRDVSRHSNAPAEAPGAAASTKAPVEPSGSAT
                     TSVIGAATASPSATPDSQLSVDGGVIELGGWRVVLMGAGERCEVLAERGAETQRLPSD
                     LRGPCYFVKDARQRVRFESYADVKSSVAILMGNPVPPKERFDVKCGSEYRASALQGVV
                     VRSTGPKLTRVMPFSRLCMDSTLDEKSYSIFAHETP"
     gene            6541..7011
                     /locus_tag="VFQ61_28495"
     CDS             6541..7011
                     /locus_tag="VFQ61_28495"
                     /inference="COORDINATES: protein motif:HMM:NF025478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system amidase effector
                     protein Tae4"
                     /protein_id="HET9958480.1"
                     /translation="MPSVQFQSLWTNHPSNLGENAPCKDKAGNSAFENQCAIRMGIAL
                     QDSGVPLAGYPGAHCWHGHSKIHALRAQELANWLATGAGARRVGAVEKKRRGAHSPIN
                     VSDYLGRTGIVFFMNFWGRGNQGDHIDVWDGSDMAHGSSDYFAASEQVWFWAIA"
     gene            complement(7073..8077)
                     /locus_tag="VFQ61_28500"
     CDS             complement(7073..8077)
                     /locus_tag="VFQ61_28500"
                     /inference="COORDINATES: protein motif:HMM:NF037384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LirA/MavJ family T4SS effector"
                     /protein_id="HET9958481.1"
                     /translation="MPLSSLTIQDEIAALVPGFDAQPTNWKMTCVRIAQFLENGAVLA
                     PNLQALETAMIALMNARLAGAAAVNPALVAQVFSNRVNQRPLAAPKLPNHMQNVLSEV
                     LGQLELANGLTLFPVGGRSASVTMTGVVQPTYFRNQISSGRPFKDPTVPLGHGEFTHR
                     IQWYLVFRTVRVPQGGWGAFYRWVGRMFAPGGLGLWDILFDRNKVGRGGANGPYDTAA
                     VTDFRSPENLHRHLRHLVACPVLRGFIEGRHRKRKNQGLIPVNKAAAPGFNNDRAMRD
                     YLSRKIFGVHYADVPIADRPTLAARTPNKVFTPGPVQAVSAETAEEARWRGIYNALGW
                     "
     gene            8494..9132
                     /locus_tag="VFQ61_28505"
     CDS             8494..9132
                     /locus_tag="VFQ61_28505"
                     /inference="COORDINATES: protein motif:HMM:NF015946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958482.1"
                     /translation="MPRFVITGQRARASADFLLQDLPSSSGRLDVLLGCLRAGLLVSH
                     GLRRESVVYLILNGGPRAPRVLRFNGAEVRFLRPDERSLAVLVQKALRTEMDHPEFTE
                     VRPGIAVAKGGLECVLNELGLSMASSLGPESLPPAYSLDERGEDLRSIVDYGPNPIFF
                     LGDDIGFDAATHERLSRLVRRTISVGPTSLYAEHALSILLNELDRRGRRSQA"
     gene            complement(9198..9632)
                     /locus_tag="VFQ61_28510"
     CDS             complement(9198..9632)
                     /locus_tag="VFQ61_28510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958483.1"
                     /translation="MKLTRGRLVLTLIGGLVVLVMSAFLLAHRHVPGPPCAALAKLGA
                     REEGNRCVVAQLNTAQYEGTLLPDHLTVRGALQIYGQRLDRLPDDLVVEGELFFYKAT
                     YPIPADITVSGNIDSYLGFGDDLLRCKDVPSTAKIKGSIRCE"
     gene            complement(9762..10961)
                     /locus_tag="VFQ61_28515"
     CDS             complement(9762..10961)
                     /locus_tag="VFQ61_28515"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9958484.1"
                     /translation="MQAFKSALWVALLLGTACGEANNEPPPPSARGRCQALDFSGVDL
                     EVEKFLGEQGLRGASSVIIDRECGQVYLKGHGQYSADRVYLLASASKVVSVGVLMWLS
                     AQGRVDLDAPIGSYLAAWGDSDKSTLTLAQLVSNSSGLVGLVDNPLYLPYACQYRPGG
                     NLSECARSIYTASDSSHRIPPDTEFHYGGGQWQLAGGVAEQATGMSWAELVRELYVDR
                     CEAESFGYTNPFALGGGIGSALRYPESFDGDVTALPQTQNPNIEGGLYASAEDYGKLL
                     LMHLRGGACSSGPVVPASELERMREDRILTAYGGSTSGMSGRTSGAGVGTGAGAFEGY
                     GLGWWIDRQHPGVFADPGLYGAFPWLDWERGYGMFFALEADSGEVGAALFSRLKPLVD
                     AAVDRAR"
     gene            complement(11047..>11567)
                     /locus_tag="VFQ61_28520"
     CDS             complement(11047..>11567)
                     /locus_tag="VFQ61_28520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958485.1"
                     /translation="LSIIHSLQSCALNAVPALTRLHDAVSVGRKRIDLTFTELELLIG
                     STYALGFWSFERSDAAEARDAQRAAFGESVSDRLKIASGLPFDRGIDAAAAECARRRL
                     PLGLDLSDAELRISSHALDVCKLEFDGNWDEFRTVFCVRSQGCLSESFSILSARLVAI
                     LQGQPASRCCIP"
BASE COUNT         2309 a         3440 c         3613 g         2205 t
ORIGIN      
        1 gcggtcaaag gctacctcaa ggcgcgaata tcgtcgctcc acgcgagctg agagcgctct
       61 cgctcggccg ctagaatttc agccctctct gaaagcttga gcgccttgag gagagcaatg
      121 ataggcgcgt gcgctcgcgt agcgaagccc cgggccctat gaaagctttg gaggcaaagt
      181 gtacagttac cacctctgga tcgtgcttgc cgaatcaact caagagaacg acatcggggg
      241 cctacaagac aagatcaacg gccttcggac cctgctggcg gagagtctcg atacgcggcc
      301 tgagcatcca atcattaaag tgaactgtga ttggctattt cagtgctcag tgtcccacaa
      361 tcataagggc gatgcgcagg agcgtctcga gagtgtcttg aggtggatta ctcgacgttt
      421 acccggcagc tacggactgg tgtactatcg ggacgacgaa gatccccggg aggcgaactc
      481 ctatcgggtc ctcgtggttt cgcgtggcgg ggtcgtagag aggacggacc cgttcctttc
      541 acccgtcgtt ccaacgattg aagactagat gaccgaagcg gcacgggaat gcaggttggt
      601 gagcgggggc ggatggcgcg acgcggagag ttggtctatg attgccgccg tgttgggccg
      661 ggaggaagac cattgaaata cgcaagtcac ttcagcacgc tgcaaactcc gcagaccgag
      721 aaagcggcgc cgaatcaggt ccaaaacaac gcgggcgggt tctcgtttca gatcactcca
      781 cagcagcagc tggagcgctg gctcgtgctc ggcagcgaag gtggcacgta ttacgtcacc
      841 gagaagaagc tgacgcgaga caacgccaag accgtaatcg cgtgcttgga cgcggatggc
      901 ccgggtacgg tctctatgat cgcgtcgttc agtgagagcg ggcgagctcc gaaaaacgac
      961 cctgcaatat tcgcgctagc gatcgcgtcc ggccactcag actcggctac gcgcaaggcg
     1021 gcgttggatg cacttccgcg cgtctgtcga acgggtacgc acttgttcca gttcgcttcg
     1081 gtggtgaaga acttccgggg gtggggcagg gggcttcggt ccgcagtcgg tcgttggtat
     1141 tcggagaagt ctcccgacca gctcgcctat cagatcgcca agtatcagca gcgcgaaggc
     1201 tggtcacatc gcgacctgat gcgattggcg catcccgggc cgaagcacgc ggcgatctac
     1261 cgttacatcg tcacgggcgc aggcgcgctc ggtgatcgag aagtgaagcg cggtgagcgc
     1321 gtctcgtcct acgccggagt gggcgagttg ccgccgttct tggccgccgt cgaggaggcg
     1381 aagcaagccg acgagcatcg aacgatcctt ctgattcggg agcacggctt gactcacgag
     1441 atggtgaact ccgagcatct ccgttcgccc gacgtttggg aggcgctact ggagcgcatg
     1501 ccgctaacag ccatgcttcg ctcgctggcg cgaatgacga acattgggct gctgaagccg
     1561 ttgtccaacg cagcgactaa ggtgggtgat acggtatcga acgaggcacg gctcaagaaa
     1621 gcccgtgttc acccgatcca ggtgctgtca gcgctcatga cgtacaagca ggggcacggc
     1681 atgagaggat cgctgacctg gtctcccgcg ccacaaatcg tggatgccct ggatgctggg
     1741 ttctacgcag cattcggggc aatcgaaccg agtggagctc gtacacttct gggcatcgat
     1801 gtgtcgggaa gtatgggcgg aagcaagctc gttggtattg acgggctcac cgcgcgtcac
     1861 ggagcggccg tgatggcgat ggtcacggcc agagtcgaga agcagtggca catactcggt
     1921 ttctccgatc gcttggtgga tttgccgatc acgccacgac agcggctcga cgatgtcatc
     1981 tcgacgatgg atcgcctgcc gttcggtcga accgattgcg ctcagcccat gctgcacgcc
     2041 gcagccaatg gcctgaatgt cgacgtcttt cacgtctaca cggacaacga gacctggtgc
     2101 gggaatatcc acccgcatca agctttgggt gcgtaccgtg ccaagaatgg aatcaattcg
     2161 aagcttgcag tgattgcttt ttcggccacc aacttctcga tcgccgatcc gtcggatgcc
     2221 cgtgagctcg acttcgtcgg gttcgacgcc gcagcacctc aggtgctagc ggactttggg
     2281 cgcgaacgga gcctcgtcgc gttcgaggga tgaagattct gcggattagt agttgcagac
     2341 tcgaagcgtg gagtctataa atcaagtgtc gcgagccgaa gctgcagggt tatctttaaa
     2401 cgattcctcc ccgcagtgcc acttgttcgc gactcacgga actggcgcct ttcccaaaag
     2461 gatgcccccc gtgcgtcatc cgtcgcccga actcgcatgc agcctgtagc tcagttggga
     2521 gagcaatagt ttgccgtaag gcatctcggg tcccaccact tgcccagttt tgggccgctg
     2581 tggattcgtt atctagaact gtgggtcgct ggttcaactc cagccaggct gccatatgta
     2641 ccgccgatgt ctctggccct agccgcagtt cgtcgtcggt tcaccagcaa taccgtacca
     2701 aggccgtcgc cagtagaggc ggagtcgcta ctggtagatt gtcgagccgc gactagattg
     2761 tcaggccgtg acatggcggg ctctggtgac ctcggctagg ctccgcgagc tcgtcgattc
     2821 ggggttcatg atggctttag ctggaagatc tactcgggcc ttggggcgac gcatactagg
     2881 cgggcttgcg gcgggttggg tcgcaggagc cgtgggctgt gcggcagacc cacctcccgc
     2941 ggcacgtgtg cccggtgcgc caatcgcgag ttcgacggcc atgacctcgt taccgatgga
     3001 tccgcgcgtg cagacgggga ggttgccgaa tgggctcact tactatctcc aacgtcacca
     3061 gcccgagcaa cggcgggccc acgtttactt cgtggtgaaa gcgggctccg tgcaagagga
     3121 ggaggatcag cgcgggctcg cgcatttcgt cgagcacatg gccttcaacg gaacccgccg
     3181 cttcgcgaag caagagctcg tggagcttct ggaaaagagc gggctcacgt tcggcgccga
     3241 catgaacgcc ggaacgactt acgatcacac tcgctatcac ctgagtgtgc cgacagatgt
     3301 tccgcatttg ctcggcaccg cactcgatgt gctcgaagac tgggcccagg cgatcacctt
     3361 catggccgac gaggtggaga aggagcgctc ggtcatcctt tcggagtgga cgagctcaca
     3421 aggggctcag cgccgcctga gtgagcagcg gctagcgttg atcctcgcag gttcgcgttt
     3481 tgctgaccgg tacgcgctcg ggaaccgtcg agtcctcgag ggggcatcgc cagagcggat
     3541 tcgggactat tatcgtcgct ggtatcgacc cgagcgcgtt gccgtcgtcg tggtgggtga
     3601 catcgagccg caagctttga agaatgagat tgaacgtcgt ttcgcgggat ggtcttcccc
     3661 ggatccgctg agtgacccca attccaatat tccggttcgg cacaccactt cactgacggt
     3721 gttgaccgat tctgaaacgc cggcggcaat cgtgagggtg ttgctcaagg cgcccgcgcg
     3781 gctcgtgcgc agcgtagaag atctgaagga ccagacccaa agccagctcg gtgcggcgat
     3841 gttgaggcat cgcttggagg cattggaaag aagcgcagaa gcttcgttta cggattcgag
     3901 ctgcagtctc accccgagcg tgctcggtcg gctggatcta ttcgaggtct tggccatggc
     3961 caagcagggc agggtacagg acactttgga gcgcctggtg atggagctcg agcgggttcg
     4021 ccgtcacggc ttcacggacg atgagcttgc ccgcgccaag gcggagcgct tgcgcttctt
     4081 cgagcacgcg ctcgccgccc agaatacgtt cgcgtccacg caggtggccg agccgttgat
     4141 taagcacttc ttgaccggcg acgtgacgcc aagtctggag cagcgggttc agttcgagcg
     4201 cagcttcgta gcggacattg tcgcggcgga cgtaagtcgg cgcatgagca ggtggctgac
     4261 ggaatccgag gagctcgtga tcgcgagcgg gccggagcgc gacgcgctgc cgagccaaga
     4321 gcagctgaat gcagcgctgg tcgccgctaa gtcacgctcg gtcgaggctt atcgcgacga
     4381 aatcccaaac attcccctga tgccgaccct gccgcagccg ggccgcatcg tgaaggaaga
     4441 tcacatcgcg gagattgggg tgagcgtctg gacgctttcg aacggcgcgc gcgtcgtgct
     4501 caagccaacg gatttcaaag ccgacgagat cctcgggcag gccattagtt tcggtggaac
     4561 ggcgactctc cctgcacgcg agttcggaga cgcgcgccta gcccacgaga tcgtgacgac
     4621 gagtggcgtg ggcgcgctcg atcgccagca gctggcgcgc gcgttggcag gtaaggccgt
     4681 caccgtgtac ccctggatcg ccgagcaaga cgagggcatt cgagcgaacg cagcgcccca
     4741 agatctcgag accatgttcc agttggtgca tctctatgcc acggcgcccc gtcgcgatga
     4801 agctgcattc gaggtgtacc gcgcgcagct cgtcaaccaa ctgcgcaatc gagacctcca
     4861 tgtgccgagc gccttcacgg acgctatcgg caatgctctt tggggcggcg acgcgagacg
     4921 tcgagttccg agcctcggcg aagtcaaggc cgtcgacttg aaccgtgctc tcgagttcta
     4981 caaacaacgg ttctcggacg tcagtgactt ctccttcgtg ttcgtcggaa agatcgatct
     5041 gaccacgttc aggcccctcg tagagcgcta cctcgcgagc ctgcccggcg gcagccggca
     5101 cgagacctcc cgagaaatag gcttacatcg taaatcgggc attacgaaag ttcggatcaa
     5161 gaagggcaaa gaagacgcgg ccgcgctctc cctgctttat catggtgagt cgccttggtc
     5221 cgagaacgcc cacactgatc tcgtctccct ccagagttac ctcgcgattc gcctgcgtga
     5281 ggtcttgcgt gaagaactcg gggccgtcta taccccctac gtcaatgcca atttcgagcg
     5341 cgtcccatac gacgcctaca gcttgactat ttcgctcgac tgcaagccct ccgacgtaga
     5401 aaaggtcctg cgcgcgaccc gtcaaatcat cgatgacacc aagaaaaccg gtgcggctgc
     5461 cggctacgtc gacaagattc gcaagcaacg aacgcgcgac ctcgaccgca tgtatcacga
     5521 gaacggcttt tggctcgagc gcctcgtaga caaatacaaa ctccgcgaag acccgcgcaa
     5581 tatcctgatc ctgcacgacc tcacgcaacg tgtgacctcc gaaaacatca agcaggctgc
     5641 cagacacttc ctccgcgagg accagtacct ggaggcggag ctccagccgg agcgctgacg
     5701 acgtcgccgc tagactcgtc ggtccgagcc tgtctctgac gtgggccgag atggtagatc
     5761 gctctacgag ccgacggggg cagcaaaatc acttggacga cggacgaaaa gcgggagata
     5821 gtgattggct ggatggtttg ggaatgggta tgaagtatgg aacacggctg gcgagcaccc
     5881 gcgacgcgcg atcgagcggg cttgctgcga tggccttcct gctggcttcc atcagtctgc
     5941 actgcgagcc acgaagctcc ccacgagacg taagccgcca ctcgaatgct cccgccgaag
     6001 ccccgggtgc ggccgcctcg acgaaggccc ccgtcgagcc gtctggttcg gcaacaacat
     6061 cggtgatagg ggcggcaacc gcgtcgccga gtgcaacccc tgattcgcag ctttccgtcg
     6121 atggcggggt gatcgaactc ggagggtggc gagtcgtgct gatgggcgct ggcgagcgct
     6181 gtgaggtcct cgctgagcgc ggggcggaga cgcagcggct gccgtcggac ctgcgcgggc
     6241 cgtgctactt cgtcaaggac gcgaggcaga gggtgcgatt cgaatcctac gctgacgtga
     6301 agagcagcgt cgccatcctc atggggaatc ccgtgccgcc caaggaacgc ttcgatgtga
     6361 agtgcgggag tgaataccgc gcttcggcgc ttcaaggtgt cgtcgtacga agcaccggac
     6421 ctaagctgac gcgagtcatg ccgttcagcc gcctttgtat ggactcgacg ttggacgaga
     6481 agagctattc gatttttgct cacgaaacgc cctagcgccc ccgggccagg agatctgacc
     6541 atgccttcag ttcagtttca aagcttgtgg accaaccatc cctccaacct gggcgagaat
     6601 gcgccctgca aagacaaagc cggcaacagc gccttcgaga accaatgcgc catccggatg
     6661 ggaatcgctc tccaggacag cggtgtcccg ctcgcgggtt atccgggagc ccattgttgg
     6721 cacggccaca gcaaaatcca cgcactccgt gcccaagaac tcgcgaactg gctggcgact
     6781 ggcgcagggg ctcgccgtgt tggcgcagta gagaagaagc gccgaggcgc ccacagcccc
     6841 atcaacgtga gcgactatct cgggcgcacc ggcatcgtat tcttcatgaa cttctgggga
     6901 cgtggcaatc aaggcgacca catcgacgtt tgggatggct cggacatggc ccatggctca
     6961 tcggactact tcgcggcctc ggagcaggtc tggttttggg ccatcgcgta gtttgggccg
     7021 tcgcgacccg ggcagggagt gcaaacctga tgaggctttt ctcgacttgc ttctaccagc
     7081 caagtgcgtt gtagatgccg cgccacctcg cctcttctgc ggtctcggcg gacactgcct
     7141 gcactgggcc cggcgtaaag accttgttag gcgtgcgcgc ggcgagagtc ggtcgatcag
     7201 cgatcggcac gtcagcatag tgaactccaa agatctttct cgataggtag tcacgcattg
     7261 ctcgatcgtt gttgaagcca ggcgcggcag ccttattgac cgggatgagc ccctgattct
     7321 tacgcttccg gtggcgtccc tcgatgaagc ctcgcaagac cgggcaagcg accaggtgac
     7381 gcaggtgtcg gtgaaggttc tctggactcc tgaagtccgt gacggccgcg gtgtcatagg
     7441 gcccgttcgc tccgccgcga cccaccttgt tgcgatcgaa caagatgtcc caaagtccaa
     7501 ggccgcccgg ggcaaacata cgccccaccc agcggtaaaa cgcgccccaa cccccttgcg
     7561 gcacacgcac ggtccgaaaa actaggtacc attggatgcg atgggtgaac tccccgtgac
     7621 ccaaaggaac agtgggatct ttgaaaggtc gcccgctgct gatttggttc cggaagtagg
     7681 tcggttgcac gacgccagtc atggtgactg atgcactgcg tccaccgacg ggaaacagcg
     7741 ttagcccgtt agccagttcg agctgtccga gcacctcaga caatacattc tgcatgtgat
     7801 tgggtagttt cggggcggcg agcgggcgct ggttgacacg gttggagaat acttgggcaa
     7861 cgagggcggg gttgacagcg gccgcaccgg caagacgcgc attcattagc gcgatcatcg
     7921 ccgtttcgag ggcctgcaag ttgggcgcga gcacggcccc gttctccaag aactgagcga
     7981 ttctcacgca agtcattttc caattcgtgg gctgcgcgtc gaatccgggt acgagcgcgg
     8041 cgatctcgtc ctgaatagtc aatgatgata atggcatgaa tggtgtccct ccacggtcga
     8101 gtggcgagtt tgtgcgaaca caggggtgga gcgcaatcag actcgctatt ccggtactcg
     8161 gtctcagcct aagtttcggt tgcggataac cacaatggca aggagttcta gtttgaatcg
     8221 gagtggcgaa ccgcccgtgc tggatggttg ctttgactcg ccctcagaaa ccagatggcc
     8281 caagattgca cgatagtagc gggactgcgg ccctcgaggt caatttacag ccattgcaac
     8341 gccgctgggg tcccggcgcc gcgccagcgg aagactctaa ctcgataaga tgcgaagcgt
     8401 cagcgtcgag tcgagcgctg tacattcccc caagtggcgc aatgggcgcg caccgcttga
     8461 cttccggcgc ggttctggcg agagcacgca gatatgccgc gattcgtcat tacaggccag
     8521 cgggcgcgcg cttcggccga tttcttactc caagaccttc cgagtagcag tggtcgcctc
     8581 gatgtgttgc tcgggtgcct gcgggcgggg ctgctcgtgt cgcacggctt acggagggag
     8641 agcgtcgtct atctgatcct caacggaggt ccacgcgcgc cgcgggtact gcgcttcaat
     8701 ggtgctgagg ttcgcttcct gcgtcccgac gagcgaagtc tcgcggttct cgttcagaag
     8761 gctctgcgta cggagatgga tcatcccgag ttcacggagg tgcgtcccgg aattgcggtg
     8821 gcgaagggag gtctcgagtg tgtactcaat gaactgggac tgagtatggc tagctcactc
     8881 ggtcctgaaa gcctgcctcc ggcctattct ttggacgaac gtggtgagga tctgagaagc
     8941 atcgtcgatt acggtccaaa cccgatattc ttcctgggtg acgacatagg cttcgatgcc
     9001 gcgacgcacg agcgactttc acgactggtg aggcgaacca tcagcgtggg gccgacgagt
     9061 ttgtatgccg agcacgcgct cagcattctg ctcaacgagc tcgatcgacg cggtcgacgc
     9121 agccaagcct gacctatgtg tacgcggctg agtgcgtggt atgagcggag accaagcgcc
     9181 ccgctgccgt tcgtcatcta ctcacagcgt atgctccctt tgatcttggc cgtgcttggg
     9241 acgtctttgc agcggaggag atcgtctccg aatccaaggt atgagtcgat gtttccacta
     9301 accgtgatat cggccggaat aggatacgtg gctttgtaga aaaagagctc tccctcgacc
     9361 acgagatcat caggcaggcg gtccaggcgt tgtccataga tctggagagc gccgcgcacc
     9421 gtcaagtggt ctgggaggag ggtgccttcg tattgcgctg tgttcaactg agcaacgacg
     9481 cagcggttgc cctcttcccg cgctcctagt ttcgccaacg ctgcgcacgg cgggccgggc
     9541 acgtgccggt gcgccagcag gaaagcactc atcactagca ccactagccc gcctatcagg
     9601 gtgagtacga gccgaccccg agtcagcttc atctgggttg ctcctcgcgc atgatcactt
     9661 atacgcttcg tggtcggagt agccaggaaa ctctaccaag acctgacgtg gatcgccccc
     9721 acagcgtcca agacggaggg tgggttctgg cgttcggcgt tctagcgggc gcgatccacg
     9781 gcggcgtcga ccaggggctt gagacggctg aacagcgcgg cacccacttc gccgctgtcg
     9841 gcctccagcg cgaaaaacat cccgtaccct cgctcccaat cgagccaagg gaaggcgccg
     9901 tagagcccgg gatctgcgaa cacaccgggg tgttgtcgat ctatccacca acccagcccg
     9961 tagccctcga acgcgcccgc gccagtaccc acgccggcgc ctgaagtgcg gccgctcatg
    10021 cccgacgtgc taccgccgta ggcggtgagg atgcggtctt cacgcattcg ctcgagctct
    10081 gaggcgggca ctacgggacc ggagctgcac gcgcctccgc gcaagtgcat cagcagcagc
    10141 ttgccatagt cctccgccga cgcgtagagc ccgccttcga tgttcggatt ctgcgtttgg
    10201 ggtagcgccg tcacgtcgcc atcgaaagac tctggataac gcagcgcgga tccgatgcca
    10261 ccacccagag cgaacggatt ggtataaccg aagctctccg cttcgcagcg gtcgacgtac
    10321 agctcccgca cgagctccgc ccaagacatc ccggtggctt gctccgccac gccgccggcc
    10381 agctgccatt ggccgcctcc gtagtgaaac tccgtgtcag gcgggattcg atgggaagag
    10441 tccgatgccg tataaatgct ccgggcacac tccgaaagat tgccgccggg gcgatactgg
    10501 caagcgtagg ggagatacag cgggttatcc accaagccca ccaatccgga gctgttcgag
    10561 accagctgcg cgagcgtgag tgtggactta tccgaatccc cccatgccgc cagataggag
    10621 ccaatgggag catccaaatc gacgcgcccc tgtgcggaca accacatcaa cactcccacg
    10681 ctcacgacct tgctcgcgga ggccagcaag tacacgcggt ccgcggaata ttgcccatga
    10741 cccttgaggt aaacctgacc acactcccga tctatgatca cactgctcgc gccgcgcagc
    10801 ccctgctcgc cgaggaattt ctcgacctcg agatcgactc cactgaaatc cagcgcctga
    10861 cagcgcccac gcgcagacgg cgggggaggc tcgttgttcg cctcaccgca agcagtcccg
    10921 aggagcaggg caacccatag agcgctcttg aaagcctgca tgtcacatca ggatatgtgc
    10981 ggagcggcac tgaaactcaa ggggtgaacc ggctcaaatg aaccttgggg tgctgatttc
    11041 gtattcttat ggaatgcagc agcgtgacgc gggttgtcct tgcaggatag caaccaggcg
    11101 cgccgacaga atggaaaacg attcggatag gcagccctga ctgcggacgc agaaaaccgt
    11161 gcgaaactca tcccagtttc cgtcgaactc gagtttgcag acgtcgaggg catgggatga
    11221 gatgcggagc tctgcatcgc tgagatcgag tccgagcggg agccttcgcc gcgcgcactc
    11281 tgcggcggcc gcgtctatgc cccgatcgaa cggcagcccc gaagcaatct tcagtcggtc
    11341 actcaccgac tctccgaacg cggcacgctg ggcgtcgcgc gcttcagcag cgtctgatcg
    11401 ctcgaaagac caaaatccaa gcgcgtaggt ggatccgatg agaagctcga gctcggtgaa
    11461 ggtgagatcg atgcgctttc ttccgacaga gaccgcatcg tgaagacggg tcagcgctgg
    11521 aacagcattg agagcgcagc tctgcagcga gtggataatt gagagca
//
LOCUS       DASTKU010000816        11129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1278045, whole genome shotgun sequence.
ACCESSION   DASTKU010000816 DASTKU010000000
VERSION     DASTKU010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11129
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278045"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3006
                     /locus_tag="VFQ61_28525"
     CDS             <1..3006
                     /locus_tag="VFQ61_28525"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9958486.1"
                     /translation="SPRFVESVLAATELLGVDGALLDVHRMASDPRGARALKAIVRPL
                     GHLIVCGRAAELDASQVGSVLRNSVNFSIADVSTLRDAQPDLCRALLREVTERFERKQ
                     LVPMEVETFSWSDASQALARLVVTNQVGWMGLNFETEAPLLAGGLEASVIRAEGTYLV
                     TGGLGGLGLAVMHWLAAEGARSIVLVGRSEPSLEARLAMEAVRGLGVSVTVVAADVSN
                     SADVARVLRIIEAELPPLRGILHAAGVLDDGMIAHQSDARLLKVLAPKVQGAWNLHQQ
                     TLHLELELFVLFSSIASVVGWAGQSNYAAGNAFMDGLAQYRRIQGLKALSVNWGPWGG
                     AGMAANLDERSAQRMREAGLSPLAADAALTAMRRLLSAQVPQAGVFEIDWASLFQFEA
                     DPARKTVFKDLLPSRALDSQGDLKQQLSALDEPERSSLVSNQVRQQLAGVLGLESGAG
                     LDPSGNVFDYGLNSLMAMDLVNRLQGALQMKLPATLVLKHPTIDAMTRCILEQLRSSE
                     APLRNQHTYWDPESSVSLAQYELNGRLATITPTVLQLMFEEHTAHFNVGMLVEIDHAA
                     FDLEALKSALNVLFTYHDGCRLRIFAENGQMQQEIAPLGAGVIVEEHDLRGLGYEAGA
                     ARMLELNEALHRSLAFTREGPLYRAAHYQLDGDPRHRFFLMFHHYVSDGWSQKLLSRE
                     LGVIYNKIVRRKPVLLPAKGYSLFDWTQRFKDFAYGEAVGQIPYWLAQLKAAEDCEIF
                     DEVPGQREPGIDDYTGESGMLEREDQIKISEYCKAHGVELADVATYAIVRAFAPLTRS
                     DALWADLITHARAGIFPDVTLPDLFGQISESGAVLFRLRPGASHLEQIAAIREQRLAV
                     PHAGLGLRALRLINRDPEVKSQILMRHAPQVGLNIDVTDYAQEASQESGVRFAREPFG
                     TASAQHQRKSPEEVRLAFFVALRQSEGRLFLSVDFYRNRFKSETIRRIIDSALDLLLE
                     IAVEGESARPVSTPPPAFSADAAGLATAV"
     gene            3025..4593
                     /locus_tag="VFQ61_28530"
     CDS             3025..4593
                     /locus_tag="VFQ61_28530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958487.1"
                     /translation="MLSVRRLCACVPVVLACTLSGNVQARPHHEIPHAFERSIASLPG
                     KSRLSLLRTLDRIDFSVDPLVNQNPRATEAVVGHETYLPNTAVQQTTRFERALLEAYL
                     NSPRDAALAKLLAAYEWFRFNHAEEDKPEGACRGHDPLRAEVAAAARRFRYGVYAEYF
                     LNRARELGAQERWIEGARSFVEQELNFVSRPESPPVVEENLESHLFFTEAFNYEEQNR
                     YEAVDKLLMDFVEQPNNVVTNAYLASSNIWLGGEAPYNDPTILYDFLLSSYFAVRSVH
                     MAEAVELAWLQDPQHNPPFRLSSILGGWVVPARRWLALVQGDNAAVEALDAEHRQWLG
                     THRAFHSASVGLMLFDEPAHVLEGFNAWMAGYEHCAEFPELRACSDRPKISFNRLSFV
                     LGGVDYLLKLGQVDAARGTLSVRFDPSYDFRYDQWELGRKEWEHRENNLEAIAALYAN
                     ADPSDDPTNFLLKSHAWGPNTIVCQTCHQTQRREWSGAEVEQYRLEPAHPDVAVVGDW
                     PEITTTWYGTTIAR"
     gene            4612..6033
                     /locus_tag="VFQ61_28535"
     CDS             4612..6033
                     /locus_tag="VFQ61_28535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958488.1"
                     /translation="MKQRVEKRTRLRTQLSALLMVAGSASIAPRALAHEPTAPKHCKP
                     SDPVAEFETFLPLENERPVARAERPLLIRYLENPRDLQVTLQLAAHHAARFAGPARNS
                     RMERSRHGIYALYFLRRAESLGVRERWTRPAIRAIDEQLTRWFRSDAALDFSEGTPAQ
                     LAFLDAFNEQERNRHRAAEQLLGEFLEHPTNLTTAIYLAAVSLWNGGESGYADPGMLY
                     SFLDAGYFGQFATELSERAEREWELDPDHRKLFRLGVTTGGMTLPARRWLAGLHGDRE
                     GVDLVDAETMQWFAKAPSFYMFPASVYAFGEPDKAASGFDALLRGLDACNTHYTVFCG
                     DNPRATFSGISFFMYLFDYFSRMGQPETAMGMLGAKANPDYKFDAWYLGQEAWAHREE
                     NATRITELYQNSDPKDDPLNTFMKRHKWGPLTSTCQTCHQTQGVKWSAAEKAQIPSLP
                     TADLYVGDWPIPTIAWDGTRVNQ"
     gene            complement(6124..7500)
                     /locus_tag="VFQ61_28540"
     CDS             complement(6124..7500)
                     /locus_tag="VFQ61_28540"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/universal stress protein"
                     /protein_id="HET9958489.1"
                     /translation="MKPASAMEAALALEPGAAIDGFTLGPRVHAGGMGYIFRVSKPDD
                     PRPLLMKLPRLGPEEPSEAIVSFETEATIAPTLQGPHVPAFVAAGDLTRTPYLVLEWV
                     DGKSLDQVLAAGPLVPLEAARVGAAVADALHSLHQQDVIHLDLKPSNLVLREDGTAVL
                     IDFGFAHHAKYPDLLAEETHFRAASAPYVSPEQLLSSREDRRSDIFALGVILYEATTG
                     RLPFGDPDTDVRNRFWLDPVPPSVLAPNVPPWFQEIILRCLEPSAEARYQSAAHVAFD
                     LRNPDQVRLTARATKRQRAGWWSHLRRFLRARAEIGPRLRVPQALLSRTPIVLVAIDT
                     SNVDDERHPAIRFAVSQILAVSAEFRLICLSVIPPAASPLEHLVRLRHWASPFQLPVQ
                     RLSLHAMESLSPADTIVEFARHNNVDLIVLGAPVEGGRAWSNSTASTVTANARCSVHV
                     VRVPAARP"
     gene            complement(7532..8272)
                     /locus_tag="VFQ61_28545"
     CDS             complement(7532..8272)
                     /locus_tag="VFQ61_28545"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9958490.1"
                     /translation="MIIALLSDIHANLPALRACLQHARERGAGQFVFLGDLVGYGPDP
                     EAVIDAVRSIPGALILKGNHDHAVEVEPKSSELNDLAYSTIEWTRTVLSAEQRQFLAS
                     LPLIVREEDRCFVHASASQPQDWEYVQDSIAARESMDAAKAQYVFSGHVHDQTLYFRT
                     QPQRTSSFKPVSGSTVPVPSRHQWLAIVGSVGQPRDGNPAAAYALFDETHQTLTFFRV
                     PYDHLATARKIRAVGLPEVFAERIERAF"
     gene            8518..9879
                     /locus_tag="VFQ61_28550"
     CDS             8518..9879
                     /locus_tag="VFQ61_28550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET9958491.1"
                     /translation="MTAKTGAPPGVAVRAARWIGWCFGVSGLIALPSRARAADGHVLS
                     LGYMHSFVYGDVPAAGNGMEGTLVFYPDSRTKFGLGGFAQLESYSPSHLRLATGFQAN
                     YSVLGFELGYAYRAESPGGKASHGLQFGPFFSTGIVSTGLRVVPTIAPGAGGYGTEVT
                     WTLAFKIPVWISGDPLGSYFTFGLPHGRPLRIGDELCFPDLQSSVHTESVAGGLPDID
                     AYLARQWSKDAQEEHASIAEFSRLSLSLMVLGAPLPLLEAAQRAALDEIEHARGAFAL
                     MARYSKSRVVPGPMPRAAEARPTSSRVELASECLRDGCLGEGVAAAVARYASERAEEP
                     ALRAFLAVISEDEARHAELSWAILEWCLETGGREVAWALSWAAGQLPLIIEPEQTEEA
                     LAAEPLESYGRASWEAQLNLFWQVRGAVLTRLSAMLAVYSDDDYSTNRALSSGVGTQH
                     WSS"
     gene            complement(9822..10265)
                     /locus_tag="VFQ61_28555"
     CDS             complement(9822..10265)
                     /locus_tag="VFQ61_28555"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HET9958492.1"
                     /translation="MQIGKQESAKQESAKQGSSEQHKVIFFDGVCHLCQASVRFIIAR
                     DPRATFQFASLQSEAAQRLLGGPVPVESVILWDEGRIYKRSAAALRIARSLRAPWPAL
                     SIFLLVPTFIRDAVYAWIAKNRYRWFGQDDQCWVPTPELKARFVE"
     gene            complement(10448..10918)
                     /locus_tag="VFQ61_28560"
     CDS             complement(10448..10918)
                     /locus_tag="VFQ61_28560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958493.1"
                     /translation="MGDIETAVRGTQQLEALLEQKFGASGRGLHEKLSSVERQLPKAL
                     VRRMRWIATVRNEVVHEAASIRDLREFKRAVTECSQQLSDTERSAVSPASSTSAARAM
                     RRERRRGSAQSGRANARSKTTSRGRRSRSRSWALRLALLALGLSGIGLYLLSSR"
BASE COUNT         2085 a         3325 c         3593 g         2126 t
ORIGIN      
        1 tccccgcggt tcgtcgagag cgtgcttgcc gccaccgagc ttctaggcgt tgacggcgcc
       61 ttgctggacg tccaccgaat ggctagcgat ccacgcggcg cgcgggctct caaagccatc
      121 gtgcggccgc ttgggcacct gatcgtgtgc ggtcgcgccg ccgaactgga cgcgagccaa
      181 gtggggtcag tcctcagaaa cagcgtgaat ttcagtattg cggacgtgtc tactttaagg
      241 gacgcgcagc cggatctgtg ccgtgctctc ctccgcgagg tcaccgagcg gttcgagcga
      301 aagcagctcg tgccgatgga ggtcgagacc ttttcctggt ccgatgcgag ccaggcgtta
      361 gctcggctcg tggttaccaa ccaagtcggc tggatggggc tcaacttcga aaccgaggcg
      421 ccgctcctgg cgggagggct cgaagcgagc gtaattcgag ccgaaggcac gtacttggtt
      481 acgggtggtc tcggcggcct gggtctcgcg gtaatgcact ggttggctgc ggagggggct
      541 cgcagcatcg tgctggttgg gcgcagtgaa ccgagcctcg aagcacgctt ggccatggaa
      601 gccgtgcgcg ggctcggtgt cagcgtgacg gtggtggccg cggacgtctc gaacagcgcc
      661 gacgtcgcgc gggtgttgcg gatcatcgag gccgagctgc cgcccttacg tgggatcttg
      721 catgccgcgg gggtgctgga cgacgggatg atcgcgcacc aatccgatgc gcgcttgctg
      781 aaggtgctcg ccccgaaggt ccagggagct tggaacctgc atcagcaaac gttgcacctc
      841 gagctggagc tgttcgtact gttctcgtca atcgcctcgg tggtgggctg ggcaggacag
      901 agcaactacg cggcgggcaa cgccttcatg gacggcctgg cgcagtatcg ccgaattcag
      961 gggctgaagg cgctctccgt aaattggggt ccgtggggcg gcgccggcat ggccgccaat
     1021 ctggatgagc gcagtgcgca gcgcatgcgc gaagctggac tctcgccgtt ggccgccgac
     1081 gcagccctga ccgccatgcg tcgtctattg agcgctcaag tcccacaagc gggggtcttc
     1141 gagatcgact gggcgtcact cttccagttc gaggcggatc ccgcacgcaa gaccgtgttc
     1201 aaagacctcc tgccgagccg ggctctggat tcgcagggcg acctgaagca gcaactctcc
     1261 gcgctggatg aacccgagcg atcgagcctg gtgtccaacc aagtccggca acaactcgcg
     1321 ggggtgctcg ggctcgagtc tggggctgga ctcgacccgt ccggtaatgt cttcgactac
     1381 ggcctgaact ctctgatggc gatggacctc gtcaatcgcc tgcagggagc actgcagatg
     1441 aagctgccgg cgacgctcgt gctcaagcat cccacgatcg acgcgatgac tcgctgcatc
     1501 ctggagcagc tgcggagctc cgaagctcca cttcgaaacc agcatacgta ctgggatccc
     1561 gagtcatcgg tgtcgctcgc tcagtacgag ctgaatgggc ggctcgcgac gatcacgccg
     1621 acggtgctgc agctgatgtt cgaggaacat acggcgcact tcaatgtcgg catgctggtc
     1681 gaaatcgacc acgcagcctt cgacctcgag gctctgaaat cggccctgaa cgtgcttttc
     1741 acctatcacg acggctgtcg tctgcgaatc ttcgccgaga atggccagat gcagcaggag
     1801 atcgccccgc tcggagccgg tgtgatcgtg gaggagcacg acctgcgagg tctcggttac
     1861 gaagcgggcg ccgcgcgcat gctcgaactc aacgaagctc tgcaccggag cctcgcgttc
     1921 actcgagaag ggccgctcta ccgagccgcc cattatcaac tcgatggcga cccacgccat
     1981 cgattctttt tgatgttcca ccactatgtt tccgacggct ggtcacagaa gctgctgtca
     2041 cgtgagctcg gcgtcatcta caacaagatc gttcgccgca agccggtgct gttaccggcc
     2101 aagggttaca gcctgttcga ttggacccag cgcttcaagg acttcgcgta tggagaggcg
     2161 gtaggccaga tcccgtattg gctcgcacag ctgaaagccg ccgaggactg cgaaatcttc
     2221 gacgaggttc cggggcagcg agagccaggg attgacgact ataccggcga gtccggcatg
     2281 ctggaacgcg aagaccagat caagatcagc gagtactgca aggctcacgg ggtcgaattg
     2341 gcggacgtcg ctacttacgc catcgtgcgc gcgttcgcgc cgctgacgcg gagtgacgcg
     2401 ctatgggcgg atttgatcac gcatgcccgc gcaggcatct tcccggatgt cacgctaccg
     2461 gacctcttcg gccaaatatc ggagagtgga gccgtgcttt tccgcctacg accgggtgcc
     2521 tctcacctcg agcaaatcgc cgcgattcgc gaacaacggc tggctgtccc ccacgctggg
     2581 ttgggcttgc gagctctgcg cctaatcaat cgagatcccg aggtgaaatc gcaaatcctg
     2641 atgcgacatg caccccaggt cgggctgaac atcgacgtca ccgattacgc gcaagaggcg
     2701 tctcaggaga gcggcgtgcg cttcgcgcgt gagccgttcg gcacggctag cgcccagcac
     2761 cagcgtaagt cccccgagga ggtgcggctc gcgttcttcg ttgctctgcg tcaaagcgaa
     2821 ggacgcttgt ttctgtcggt ggacttctat cgcaaccgat tcaagtccga gacgattcgc
     2881 cgaatcatcg attccgcact ggatctgttg ttggagatcg cggtggaggg cgagtcagcg
     2941 cgccctgtat ccacgcctcc gcccgcgttt tctgcggatg ccgccggtct cgcaaccgcg
     3001 gtctagaaat tggagcccac taatatgcta tctgtccgtc gcctttgcgc ctgtgtcccc
     3061 gtggttttgg cctgcacgct ttccggtaac gtccaggccc gaccgcacca tgagatcccg
     3121 cacgctttcg aacgttcgat cgcatctctt cccggcaaga gtcgattgag cctgctgcgt
     3181 actttggacc gcatcgactt ctccgtggat cctttggtga accagaaccc gagagccacc
     3241 gaggcggtgg tgggtcacga gacttacctg cccaacactg cggttcagca gactacgcgg
     3301 ttcgagcggg ctttgctcga agcttacttg aactctcccc gagatgcggc gctcgccaag
     3361 ctgctggcgg cctacgagtg gttcagattc aatcacgccg aggaggataa gcccgagggg
     3421 gcgtgccggg gtcatgaccc gttgcgagcg gaggttgccg cggccgcgcg gcgcttcagg
     3481 tatggcgtct atgcggagta ttttctcaat cgagcacgag agctgggcgc gcaagagcgc
     3541 tggatcgagg gcgcgagaag cttcgtcgag caagagttga acttcgtctc tcgtcccgag
     3601 agcccaccgg tcgtggagga aaacctcgag tcgcacctct tcttcacgga ggcattcaac
     3661 tacgaggaac agaatcgcta cgaggcggtg gacaagctgc tcatggactt cgtcgagcag
     3721 ccgaacaacg tcgtcacgaa cgcctacctc gcttcctcga acatctggct cggtggagag
     3781 gccccctaca acgatcccac gattctctat gacttcttgc tgagtagcta ctttgcagtc
     3841 cgatccgtgc atatggcgga ggccgtcgag ctcgcttggt tgcaggaccc gcaacacaat
     3901 ccgccgtttc ggctgtcctc gattttgggt ggatgggtcg tgccggcgcg gcgttggctc
     3961 gcgttggtgc agggggataa cgccgcggtg gaggcgctag acgcagagca ccggcaatgg
     4021 ctggggacgc atcgcgcgtt ccacagtgca agcgtgggcc tgatgttgtt cgacgagccc
     4081 gcccatgttc tcgagggctt caacgcgtgg atggccggat acgagcactg cgccgagttc
     4141 ccggagctgc gggcctgtag cgatcgcccg aagattagct tcaaccggct gtcgttcgtg
     4201 ttgggcggcg tcgactacct gctcaaactg gggcaggtgg acgcggcccg aggcacgttg
     4261 agcgtgcgct tcgacccaag ctacgacttc cgttatgacc agtgggagct cggacgtaag
     4321 gaatgggagc accgcgagaa caacctcgag gcgattgccg cgctgtacgc gaacgcggat
     4381 ccaagtgacg acccgacgaa cttcctgctc aagtctcatg cttgggggcc aaacacgatc
     4441 gtgtgtcaga cctgccacca aactcagcgc cgagagtggt caggggccga ggtcgagcaa
     4501 tacagactgg agcctgccca cccggacgtc gccgtggtgg gggattggcc ggagattacg
     4561 accacctggt acggcacgac gatcgcacgt tgaagatgga gggagacgag catgaagcag
     4621 agagttgaaa agcgaacgag attacgaacc caattgagcg cgttactgat ggtcgcgggc
     4681 tccgcaagca tcgccccccg ggcgctcgct cacgagccta cggcgcccaa gcactgcaaa
     4741 ccgagcgatc cggtcgcgga attcgagacg ttcttgccgc tcgagaatga acgcccggtg
     4801 gcgcgcgccg agcgacccct tctgattcga tatttggaga atccgcgcga tctccaggtg
     4861 accctgcagc tcgccgcaca tcacgccgcg cgtttcgccg gccccgctcg aaattcgagg
     4921 atggaacgca gccgacacgg catctacgca ctctactttc tgcgtcgcgc ggagtcgctg
     4981 ggcgtgcgtg agcgctggac gcgccccgcg attcgcgcca tcgacgagca gctgacacgt
     5041 tggttccgga gcgatgcagc gctcgacttc agcgagggca cgccggctca gctcgcgttt
     5101 ttggatgcct tcaatgagca ggaaagaaat cgtcatcgcg ccgcggagca gctgctcgga
     5161 gagtttctgg aacacccgac gaaccttacg accgcaatat atctggccgc ggtgagcctc
     5221 tggaacggag gagagtcagg ctatgccgat ccgggaatgc tctattcctt tctggacgcg
     5281 ggttactttg gacagttcgc gacggagcta tccgagcgtg cagagcgtga gtgggagctc
     5341 gatccggacc accggaaact cttccggttg ggcgtgacca ccggcgggat gaccttgccg
     5401 gcgcgacgtt ggttggccgg ccttcacgga gatagagagg gcgtggatct cgtcgatgcc
     5461 gagacgatgc aatggttcgc caaggcgccg agcttctaca tgtttcccgc gagtgtctac
     5521 gcatttggcg agccggacaa ggccgcttcg ggattcgatg ctctgttgcg cggcttggat
     5581 gcttgcaaca cccactacac cgtattctgc ggagataacc cgcgagccac gttcagcggg
     5641 atctccttct tcatgtatct attcgattac ttctccagga tggggcagcc cgagacagcg
     5701 atgggaatgt tgggagccaa ggcaaatccc gactataaat tcgacgcctg gtacttgggg
     5761 caagaggctt gggcccaccg tgaggagaat gcaacccgca tcaccgagct gtaccagaac
     5821 agcgatccga aggatgatcc gctcaacacg ttcatgaagc ggcacaaatg ggggccgctc
     5881 acatccactt gccaaacttg ccatcagacg caaggcgtga agtggagtgc ggccgagaag
     5941 gcccagattc cgtccttgcc gacggctgac ctttacgtcg gcgattggcc tattccaacg
     6001 atcgcgtggg acggaacccg cgtaaaccaa taagagttcc tctcggtatc gactcgcgcc
     6061 cgtcccgtgg gaacgctgcc agcactctcc ccgggacgga agggcgcgga agggtggggg
     6121 gcctcaaggg cgggctgcgg gcacgcgcac aacgtgtacg ctgcaacggg cgttcgcggt
     6181 caccgtggag gccgtggagt tcgaccacgc acgcccaccc tccaccgggg caccgagcac
     6241 gatgagatcg acgttgttgt gccttgcgaa ctccacaatc gtgtcggccg gtgacagcga
     6301 ctccatagcg tgcaaggaca gccgctggac ggggagctgg aacggcgatg cccagtgccg
     6361 caaacgaacc aagtgttcca acgggcttgc ggcgggcgga atgacgctca ggcaaatcag
     6421 acggaactcg gcgctgaccg ccaagatctg tgagacagca aaccgaatcg ctgggtgccg
     6481 ctcgtcatct acgttcgaag tgtcgatcgc aacgagcacg attggggttc ggctgaggag
     6541 tgcttggggc acgcggagcc gtggcccgat ctccgcgcgg gcccgcagaa agcgtcgaag
     6601 atgcgaccac cagcctgccc gttggcgctt cgtggctcgc gccgtgagcc ggacttggtc
     6661 cgggttcctc aagtcgaagg cgacgtgagc cgcggactgg taacgcgcct cggcactcgg
     6721 ctccagacag cgcaagatga tctcttggaa ccaaggcgga acgttgggag cgagcaccga
     6781 gggtggcact ggatcgagcc aaaaccgatt ccgcacgtcg gtgtcagggt cgccgaacgg
     6841 cagccgtccc gtcgttgctt cgtataggat cacccccaac gcgaagatgt cactgcgacg
     6901 atcttcgcga gagctcagca gctgttccgg ggatacatag ggcgcagacg cggcgcgaaa
     6961 gtgcgtttcc tcggcgagta gatcggggta cttggcgtgg tgcgcgaagc caaagtcgat
     7021 gagtaccgcg gtgccgtcct cacgcagcac gagattcgat ggcttcaagt cgaggtgaat
     7081 cacgtcttgt tggtgcaagc tgtgtagagc atcggcgacc gcggcgccga cacgcgccgc
     7141 ctcgagtgga accagtgggc ccgcggcgag aacttgatcc aagctctttc cgtccaccca
     7201 ctccaacacc aaatagggag tgcgcgtgag atctccggcc gcgacgaagg ctggaacatg
     7261 agggccctgc agggttggag cgatcgtggc ttccgtctcg aagctcacga tggcttcact
     7321 cggctcctcg ggtccgagcc gaggaagctt catcagcagc ggtcgaggat catccggctt
     7381 gctgacacgg aagatgtatc ccatcccacc cgcgtgtact cgtggcccaa gcgtgaagcc
     7441 gtcgatggca gcgccgggtt ccagggctag tgcggcctcc atggctgacg cgggtttcat
     7501 ggctgcgggc ctccgctgat cggacggcat ctcagaaggc tctctcgatt ctttccgcaa
     7561 acacctctgg taagccgacg gcgcggatct tgcgagcggt cgccaggtga tcgtatggga
     7621 cccggaagaa ggtcagtgtc tggtgggtct cgtcgaacaa cgcatatgcg gcggcggggt
     7681 taccgtctcg cggctgcccc acggagccga cgatggcgag ccactggtgt cgagatggaa
     7741 cgggcacggt gctgcccgaa acaggcttga aggaggaggt tctttggggt tgagttctga
     7801 aatatagagt ctgatcgtgc acgtgtccgg agaacacgta ttgggctttc gcggcgtcca
     7861 tgctctcgcg ggccgcgata ctgtcttgaa cgtattccca atcctggggc tggctcgcgg
     7921 aggcgtgcac gaaacaacgg tcttcctccc gcacgattaa tggaagcgag gcgaggaact
     7981 ggcgttgctc ggccgagaga acggtgcgtg tccactcgat ggtcgagtag gccaagtcat
     8041 tcagctcgct cgacttgggc tcgacttcga ccgcatggtc atggttaccc ttgagaatca
     8101 gggcgcccgg aatcgagcgt actgcatcga tcaccgcttc cggatcggga ccgtagccaa
     8161 cgaggtctcc cagaaacacg aattggcccg cgccgcgctc tcgcgcatgt tgcaagcagg
     8221 ctcgaagagc gggcaaattg gcgtgaatat cggagagcaa cgcgatgatc atgggtgatc
     8281 ggaggggagg ctcaatcctg acggacagga ggccgcgctg gcagtcaggc tactcctgcg
     8341 tagccggttt gaaagggctt tctggagtgg cttgcttcga tccgagacca aacgcggaag
     8401 cggcagcgtc atgtggcgcc aggcgtgcgc aacggctagg ttgaacgcgg cgtgcaggcg
     8461 tggtcacctg ctggaatgca ggacggaggt tccacaaggg acaacgaagc gcggcgggtg
     8521 acggccaaga cgggggcgcc tcccggtgtt gcggttcggg cggcccgctg gatcggttgg
     8581 tgctttggcg tttccggcct gattgcccta ccgagccgcg cgcgcgccgc cgatgggcat
     8641 gttctcagcc tcggttacat gcactccttc gtgtacggcg atgtgcccgc agccgggaac
     8701 gggatggagg gcacgttggt gttttatcca gattccagaa cgaagtttgg cttgggtggc
     8761 ttcgctcaac tcgaatcgta ttcgccgtcg cacttgcgcc tcgccactgg gtttcaggcg
     8821 aactactcgg tgctcggctt cgagttgggc tacgcatacc gggcggagtc tcccggcggc
     8881 aaagcgagcc acggtttgca attcggaccg ttcttttcga caggcatcgt ctcgacgggg
     8941 ttgcgtgtgg tgcccacgat tgcacccggg gctggtggat acggcaccga agtgacgtgg
     9001 acccttgcct tcaagattcc ggtgtggatc tcgggagacc ccctcggaag ctatttcacc
     9061 ttcggcttgc ctcatggccg gcccttgcgg atcggggatg aactttgctt ccccgacctt
     9121 cagagctccg tccataccga gtcggttgcg ggcggcctgc cagatatcga tgcatatctc
     9181 gctcgccagt ggtcgaagga tgcgcaggaa gagcacgctt cgatcgcgga gttctcgcgc
     9241 ctcagcttgt ccttgatggt actcggggcc cccctcccgc tattggaggc cgcgcaacgc
     9301 gctgccctcg acgaaatcga gcatgcccgc ggagcctttg ctctgatggc tcggtatagt
     9361 aaatcccgag tcgttcctgg gcccatgccg agggcggcag aggctcggcc aacctcgtcg
     9421 cgcgtcgagc ttgcctccga atgcctgcgc gacggttgcc taggagaggg cgtggcggcc
     9481 gcggtggcgc gttacgccag cgagagagcg gaagagcccg cgcttcgggc ctttcttgcc
     9541 gtgatttccg aggacgaagc gcgtcacgct gagctctcct gggctatcct cgagtggtgt
     9601 ttggagactg ggggaagaga agtggcttgg gccttgtcgt gggcggcggg acagctcccc
     9661 ttaatcatcg agccggagca aaccgaggaa gcgctcgcgg ccgagcccct ggagtcctac
     9721 ggccgtgcga gttgggaggc gcaactaaac ttgttctggc aggtgcgagg cgcggtcctt
     9781 actcgattgt ctgcgatgct ggccgtgtac tcggacgacg attactctac gaatcgcgcc
     9841 ttgagctcgg gcgtaggcac ccaacactgg tcatcctgac cgaaccaacg atagcgattc
     9901 ttggcaatcc acgcgtacac cgcatcgcgg atgaaagttg gcaccaacag aaagatcgaa
     9961 agcgccggcc aaggcgcgcg tagggagcgc gcgatgcgca gcgctgcagc ggatcgttta
    10021 tagattcgtc cttcgtccca aagaatcacg ctctctaccg ggacgggccc tcctagcaga
    10081 cgctgagcgg cctccgactg cagtgaagca aactggaacg tggcgcgcgg atccctcgca
    10141 atgatgaagc gtacggatgc ctggcatagg tggcaaactc catcgaagaa gatcacctta
    10201 tgctgttctg aacttccttg tttcgcgctc tcttgcttcg cgctctcttg ttttccgatc
    10261 tgcactcgat gaactcaccg ctgaaggagg actctgggcg agaagagttt ggcacggcgc
    10321 ggctcgtctt gcgacaggtg acctcgtagc ggatcgtctg tcggcggggt gaggcaggaa
    10381 agagctgtgg cattcacggc aagcttggtc caaagcgcag aatctcctca gcagatatcg
    10441 cccatcctca gcgggaagac agcaagtaca acccaatccc cgaaagaccc agggcgagca
    10501 gcgccaagcg caaggcccag ctcctggagc gggagcgcct gccacgtgac gtggtcttgg
    10561 agcgagcgtt ggctcttcct gattgcgccg acccgcgacg ccgctctctg cgcatcgctc
    10621 tagccgcaga cgtagatgaa gccggagata cagctgaacg ctctgtgtcg gagagttgct
    10681 gggagcactc ggtcaccgct cgcttgaact ctcgaaggtc tcggatcgac gctgcctcgt
    10741 gaaccacctc attgcgcacc gtcgcgatcc aacgcatccg acgcaccagg gccttgggca
    10801 actgacgctc gacgctggag agcttctcgt gaagcccgcg tccagacgca ccaaactttt
    10861 gttcgagcag agcctcgagc tgctgtgtgc ctcggacggc tgtttcgatg tcacccatgg
    10921 catgcgctca tgacgacgag ctcattacgt ttggcggctc gcgtcaacgg gctcgagtga
    10981 tcgaggagac ctaatgccta cgggttctcc tggtgttcaa tcactctccc agtagcgcac
    11041 gggtgtacga attcgagaac aagccgccga ggtcgagtct tcttcgaaat gcgttgaagg
    11101 cttgcacgcg cgcgccacgt cgcgtccga
//
LOCUS       DASTKU010000817         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1278046, whole genome shotgun sequence.
ACCESSION   DASTKU010000817 DASTKU010000000
VERSION     DASTKU010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278046"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFQ61_28565"
     CDS             <1..541
                     /locus_tag="VFQ61_28565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958494.1"
                     /translation="ALDRAEAAFDRGELEESIRQAQTAATLQLPGAAHVDLAYTRLIA
                     IARGAEAVGRPHLAARAWSAIREAAVETRHPLLESRPELEAAEQNLARLAAASQSREP
                     EGPVEQRLLAGFARPTQREGDSAIGLGLGLVVAVIGLFWTGARGVTREGKWNARHVLV
                     GVLCSLIGAACWTWAAYRA"
     gene            550..1482
                     /gene="xerC"
                     /locus_tag="VFQ61_28570"
     CDS             550..1482
                     /gene="xerC"
                     /locus_tag="VFQ61_28570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HET9958495.1"
                     /translation="MTAEPDQNALVGFIIAFLEYLQHERRASRHTVAAYGRDLRNFAE
                     FVAERGAGASLRRLDRGVLRAYLGELGKDRTSTTLARKVSSLRAFFGYLERRGHISKN
                     PASALATPKLARKLPKFLDPEAAAAVMDAPQSGSEPERDRAMLELLYGSGLRVSELAG
                     LNVADVDLAAMQVRVLGKGNKERVVPLGSKAAEAVSQYLQHRAVEPKTNGPLFVGRRG
                     RRLGVRWIQKLVHRYGALGAGRPDLHPHALRHSCATHMLEGGADLRAIQELLGHSSLS
                     TTQRYTHVSMDQLVAVYDRAHPWAHKNPKKPNEP"
     gene            1479..>3057
                     /locus_tag="VFQ61_28575"
     CDS             1479..>3057
                     /locus_tag="VFQ61_28575"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9958496.1"
                     /translation="MRLRAFGASLAGATFGCLIAAALDARFAVQRQPAPWLAAFAVSF
                     GTLVPLALVFAGVGWATRILLLDSGPVPSSAGVESARVEHTPTQSARTEEQPHWLRGI
                     ALRLSPSEAAARRELAVVCAVAPWFAFLGLLALSRTALSALASSETASGSGVRLGLTT
                     LVVLGTGWLWIEALARYGGARAWRAPSPLFCLVVSAAVMSIVFGLAVATGAPSGAGGA
                     WQMLGVLNRDELDLRPVGLIGLIACSSVLWPEPAGERFAFPVSASSFGLWIGSLAFTF
                     HAARGGFDQRSVALAIETAAPLAGKLVPSLRKRFDRDHDGYSAYFGGGDCDDGASAVN
                     PLAEDVPGNGRDEDCSGSDASALAAPAPSAPTAPSGAPKLPDRLNVVLLTVDTLRYDL
                     GYAGNPRKISPKLDELAAESVVFERAYSLASYTAKSLPPMLIGKYSGETHRGWSHFNR
                     FEKADLFVAERLQRAGVRTVSVQGHWYFFQNFGMERGFDVIDSSAAPRALQAAEGDRS
                     STSEAVSNAVIGQLEKLA"
BASE COUNT          479 a          935 c         1061 g          582 t
ORIGIN      
        1 agccttggat cgcgcggagg ccgcattcga tcggggagag ctcgaagagt ccattcgcca
       61 agctcagacg gcggccacgc tgcaactgcc tggggcggca cacgtggact tggcttacac
      121 gcgcctgatc gccattgcgc ggggcgcgga ggcggtaggc cgcccacacc tcgcagctcg
      181 cgcctggagt gcgatccgag aggccgcggt ggagacgcgc catcctctgc tggagtctcg
      241 gccagagctc gaagcggcgg aacaaaatct cgcgcggttg gccgccgcgt cccaaagccg
      301 agaacccgag ggcccggtcg aacaacgcct gctcgcaggc tttgcccggc ccactcagcg
      361 ggagggtgac agcgccatcg gcctggggct tggcctagtg gtcgcggtga tcggcctttt
      421 ctggacgggt gctcgaggcg tcacacggga aggcaagtgg aacgcccgcc acgtgctggt
      481 tggcgttctg tgctctttga tcggcgcggc ttgctggacc tgggcagcgt accgagcata
      541 actgctgtcg tgaccgcaga gcccgaccag aatgccctcg tcggtttcat catcgcgttt
      601 ttggagtacc tgcagcacga gcgtcgcgcc tcgcgccaca cggtggccgc gtatgggaga
      661 gatctgagga acttcgccga attcgtggct gagcgtgggg ctggcgcgtc attgcgccgt
      721 ctggaccgtg gtgtgctgcg cgcctatctg ggcgagctcg gaaaagaccg gacgtccacg
      781 acgttggcgc gcaaagtctc cagtttgaga gctttctttg gatatctcga gcggcggggt
      841 cacatctcga aaaatcccgc gagcgcgctc gcgaccccga agctcgcgcg caagctgccc
      901 aagttcttgg acccggaagc cgcggcggcc gtgatggacg cgccacagtc aggctcggag
      961 ccggagcggg accgagccat gctcgaactg ctctatggct cgggtttgcg ggtgagcgaa
     1021 ttggcgggat tgaacgtcgc agacgtggac ctggcggcca tgcaggtgcg cgtgttgggc
     1081 aagggcaaca aggagcgggt ggttccactc ggtagcaaag ccgccgaagc cgtaagccaa
     1141 tacctgcagc atcgcgccgt cgaacccaag acgaatgggc cgctattcgt cgggcgtcgg
     1201 gggcgacggc tcggcgtgcg ttggatccaa aagctcgtgc accgctacgg agcgcttgga
     1261 gcgggtcgcc ccgatttgca tccgcatgct ctgcgtcatt cctgtgcgac ccacatgcta
     1321 gagggcggag cggatttgcg ggccattcaa gaactgctcg gtcactcgag cttgtccacc
     1381 acgcagcgtt atacgcatgt ttcgatggac cagctggttg ccgtctatga tcgcgcccac
     1441 ccttgggccc acaagaatcc caagaagcca aacgaaccat gagattgcga gcgttcggcg
     1501 caagcctggc aggcgctacc ttcggctgtt tgattgcggc cgctctcgac gcgcgtttcg
     1561 ccgttcaacg ccagcctgcg ccttggctcg cggccttcgc cgtcagtttc ggcacgctcg
     1621 tgccattggc gctcgtgttt gcgggcgtgg gttgggccac gcgtatcctt ttactcgatt
     1681 caggccccgt gccgagctct gcgggcgtgg agagcgcgcg ggtggagcac acgccgacgc
     1741 agagcgctcg cacagaagaa cagccccact ggttgcgcgg gattgccctg cggctgagcc
     1801 cgagtgaggc ggccgcgcgt cgcgaactcg cggtggtctg cgctgtggcc ccctggttcg
     1861 cctttctcgg cttgctagcg ctgtctcgaa ctgcgctcag cgctctggca tcatcggaaa
     1921 cggcgagcgg ctccggcgtt cgcctcgggc tcaccacgct ggtggtcctc ggcaccggct
     1981 ggttgtggat cgaagctttg gcgcgttacg gcggggctcg ggcctggcgc gcgccgagcc
     2041 cgttgttctg cctcgtggtc tccgcggccg taatgagcat cgtctttggc ttggcggttg
     2101 cgacgggcgc gccgagcggg gcgggggggg cctggcaaat gctgggtgtg ctgaaccgag
     2161 acgagctcga tctgcggccg gtggggctga tcggattgat tgcttgctcg agtgtgctct
     2221 ggcccgagcc ggcgggagag cgttttgcct ttcccgtctc ggcctcgtcg ttcggtctat
     2281 ggatcgggag cctggccttc acgttccatg cggcgcgtgg ggggttcgat cagcgttcag
     2341 tggcactcgc catcgaaacg gcggcaccgc tcgcggggaa actcgtgccg agtctccgta
     2401 agcgattcga tcgcgatcac gacggatatt cggcctattt tgggggagga gactgcgacg
     2461 atggggcgag cgcggtgaac ccgctcgcgg aggatgttcc cggcaacggt cgggacgaag
     2521 attgctccgg ttcggacgcg agcgcgttag cagctccggc gccatcggct ccgactgctc
     2581 cttcgggcgc gccgaagctt cctgatcgct tgaacgtggt tctgctcacg gtcgatacgc
     2641 tgcgttacga tttgggttac gcgggcaacc cgcgcaaaat ttccccaaag ctcgacgagc
     2701 ttgcagccga gagcgtggtg ttcgagcgcg cgtattcgct ggcctcctac acggccaaga
     2761 gcttgccccc gatgttgatc ggcaagtact ccggtgagac gcaccgcggt tggtcgcact
     2821 tcaaccgctt cgagaaagcg gacttgttcg tcgcggagcg gctgcagcgc gccggtgttc
     2881 gcaccgtgag tgtgcaaggg cactggtact tctttcagaa cttcggcatg gagcgcggct
     2941 tcgacgtgat tgacagctcc gccgcgccgc gcgccttgca agcggccgaa ggggaccgca
     3001 gcagtaccag cgaggcagtt tcgaacgcgg tgattggaca gctggaaaag ttagcgc
//
LOCUS       DASTKU010000818         5044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_161200, whole genome shotgun sequence.
ACCESSION   DASTKU010000818 DASTKU010000000
VERSION     DASTKU010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161200"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            556..1407
                     /locus_tag="VFQ61_28580"
     CDS             556..1407
                     /locus_tag="VFQ61_28580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958497.1"
                     /translation="MNKIGLIISSAIGLLGLASACSVEPGTAEDMASADQSVCANPEG
                     ANYSMAGLAAATAKELRRWNTAKDFQVVVKCNYSMAGCQEVVDLTSTGKAQCADGQCK
                     NVQAILDFQKKEASGKIKFPGGATLQSDIFAQRLVANLKAQITCNSKPDNHQADNCPV
                     EQHTLTFLSANPGGCETDYWFRPYKAGTTTPLTYPAQLKNQLITFGSLAGNPYLAFGT
                     SGQDVKIDPGPGTVGGDPSTTGSCPVLTMNGKFSTTDIVGQCCIFNNVQMKFARSTFN
                     ASYYLCQ"
     gene            1891..3771
                     /locus_tag="VFQ61_28585"
     CDS             1891..3771
                     /locus_tag="VFQ61_28585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958498.1"
                     /translation="MRLSSAWQRTSAWRVLSGLAGALLWSSSCSLVYDLSPDQCGATP
                     ECTGRFGAGYVCEAGLCKKTGSGGRTSTGGGGSGGTSNSSGSSGLGGSSGGSGGRTSG
                     GGTAGSSDETGGTGGEPFVPECDTHAECLTKYGDVDPRACVEGTCVLLKSNECPLILP
                     LKGDQWLDNLKNSNAIVMGVYSPIPPASLLSNYTRFADLALTEETEEVKGLPGPNGKR
                     RQIVMVVCDGGTPTTDQLRASGRHLMEELKVPGIVSNLQSADLQFVFEEYAQAAGTFV
                     MSALDADDSILNLQDEGRVWTMLAGPDQVAVTVPPLLDRTIDYLRSKSVLEESENVRV
                     ALVAPTGEPRLLGDMAEAIRKTIRFNGKSALDNFPDNFKLIDIETVYGDAEAAKDQSG
                     VVSELLGFKPHVILAMATNEFLSTVIPGLEGQWSSAAAGQARPFYVLSPYHLGNVAMP
                     TALGAESTVRTRMVGVGYAAAEDRSVYQDYILRLDQAYPEIQKDSSRLGLENFYDAPY
                     YLIYAAAAAGNPPQLTGDLLAKGMTRLIKGNTSYEIGRDDMVEAFGFLQANAKNTITL
                     KGTLGPANFDESTGARADGGSVFCMDTSNKFAPDILRWNSASEMFVDAATPIGTACIP
                     GF"
     gene            3771..4934
                     /locus_tag="VFQ61_28590"
     CDS             3771..4934
                     /locus_tag="VFQ61_28590"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9958499.1"
                     /translation="MSRWFAPFACGIALTLGGCSAPVSFDEEGTVEQTIVNGEASGPE
                     DDFTVDLYSGPPTARAHCSGALIAPNLVITALHCVAYISDAPFACQNDGTLSTPPPGG
                     YLGATDDPKTIDVHLGAAHDEVPEAGVLRVFGTGSTHICRHDLALLLLDRELDAPIAR
                     LRFGSPIERGRRLRLIGFGDYGVSGAPITRHVRAGARVTDVGQTDSTAAVGTAAPETF
                     VVTEGACQGDSGGPAMTEDTGAIAGVYSISAAATCTATGVRNVYTGLYPFEDFIRQAF
                     EVAGHEPLLEGQGEGGAGGSTGTGASNSGGASTSGGGVTTGGTTTHSAGTSGEEPEPE
                     PETPGSGSRRDPSCACRVTPAGGAGPQPSAWAWPVALAVAGLLGRRASRNRTR"
BASE COUNT          931 a         1538 c         1647 g          928 t
ORIGIN      
        1 gagcgggcgc ctagggtccg cagccttcga gcacctgccg gtactctccg cggcgtcatc
       61 ccgagagctg gcgtgcgacc cagcgagaga gcagcggtct atccactacg acgccaacaa
      121 tctcgggacg gcatcctgag acacgggaga tcgagtgtga tgtggggttg gcacggacct
      181 tctcagccgt ctcggccctg agattgacag catgatttcc cgtcattttt cgaggtcagg
      241 cgttcgttcg gtgcgcgcgt tgtgcaacgc gcgccgcacg agttgtgcaa cgtgtgatgc
      301 acaactcgcg cacccgccat cgccgcacgt tcgagccaag tctgagcggg tgcaggcgga
      361 gcgagggcta cgtagcgact cgagcaacga tcagcgcaag cccagtttca tgcgcatttc
      421 ctgcattgaa tcagttcgga acgacgtggc tccccagccg tgcactcgag ttgcgagaag
      481 cgctctccaa taaccccgcg gcatgagagc tgcagaaggg atgagcagct cgcaagcaaa
      541 ggagagcagc ataagatgaa caagattgga ttgattatct cctccgcaat tggtcttctc
      601 ggtcttgctt cggcctgctc ggtcgaaccc ggaaccgcag aagacatggc ttctgcggac
      661 cagtccgtgt gcgccaaccc cgagggtgca aactactcga tggcgggctt ggcagccgcg
      721 actgcgaaag agctgcggcg ctggaatacg gccaaggact tccaggtcgt ggtgaagtgc
      781 aattactcga tggcgggctg ccaagaagtg gttgacctga cctcgaccgg aaaagcccag
      841 tgcgctgacg gccaatgcaa gaacgtgcaa gcgattctgg acttccagaa gaaggaagcc
      901 agcgggaaga tcaaattccc gggcggcgcc actcttcaga gtgacatctt cgcccaacgt
      961 ttggtcgcca acttgaaggc gcagatcacc tgcaacagca agcccgacaa ccatcaggcc
     1021 gacaactgcc cggtcgagca acacacgctg accttcctga gcgccaaccc cggcggctgt
     1081 gagaccgact actggttccg cccctacaag gcgggaacca cgacgccgct cacctatccg
     1141 gcgcagctga agaaccagct aatcacgttc gggtcgttgg cgggtaatcc ctacctcgcc
     1201 ttcggcacga gcggccaaga cgtgaagatc gaccccggtc ccggtaccgt gggcggcgac
     1261 ccgtctacca cgggtagctg tccggtgctg acgatgaacg gcaagttcag cacgaccgac
     1321 atcgtgggcc agtgctgtat cttcaacaac gtgcagatga agtttgctcg ctctaccttc
     1381 aacgctagct actacctgtg ccagtgaacg gccgggtaga gtcattgggt tgtagtcgct
     1441 agcgagctcc gaacgggagc ggaatgggcc gtgcagccca ctccgctccc ttcggttttt
     1501 tgtacgtccc gtagaccgat gaacgactcg acccgcgcgc ggcgcgctcc tgtgggtgac
     1561 tgtactgagc ttgactagac tgagctcctg ggttgtatcc gaacccgact cacggatcag
     1621 cggttcggct ttcgatcggc gctgctgcgg gcattgagcg cgcaagtaac cagcgaggat
     1681 atcgagtcgg cgcgctatta agcttggcgt gcgagctgtc gtcctggtat cagaatgaag
     1741 cgtcggattg cgacacaccg actccgtcga tacggttcgt cagtgcggtc cgtcggtgcg
     1801 gttcgaacgt ggccccaccg cgagcaacgt gcgcaatcag cactttgcgc gcgatctttc
     1861 aagtgacttc aattgagcta gtggtcttcc atgcgcttgt cttcggcttg gcaacgaact
     1921 tcggcttgga gagtactgtc cggtttggcg ggagctctgc tctggtcttc gagttgttcg
     1981 ctcgtctatg acttgagccc ggaccagtgc ggcgcgacac ccgaatgcac gggtcgcttc
     2041 ggcgcgggct acgtgtgcga agcgggcttg tgcaagaaaa ccgggagcgg tggccgtacc
     2101 agtaccgggg gcggcgggag cggcggcacc tccaattcct cgggctcgag tggcttgggg
     2161 ggcagcagcg gcggcagcgg tggtcgtacg agcggcgggg gcacggccgg cagcagcgat
     2221 gaaacgggtg gtacgggcgg cgagcccttc gttcccgaat gcgatacgca tgccgagtgc
     2281 ctcaccaagt atggcgatgt cgatcctcgc gcttgcgtcg aggggacctg tgtattgctg
     2341 aagagcaatg agtgccccct gatcttgccg ctcaagggag atcagtggct cgacaacctg
     2401 aagaatagca acgcgattgt catgggcgtc tactcgccca ttcctcctgc gtcactgctc
     2461 agcaactata cgcgcttcgc ggatttggct cttacggagg agaccgagga ggtcaagggt
     2521 ctgcccggtc ccaatggcaa gcgccgtcag atcgtgatgg tggtctgtga cggtggcacc
     2581 ccgaccacgg atcaacttcg cgcgagcggc cggcacctga tggaagagct caaggtgccg
     2641 ggcatcgtct ccaatctgca gtcggcggat ctgcagttcg tgttcgaaga gtacgctcag
     2701 gccgcgggca cgttcgtcat gagcgcgctc gacgccgatg actcgatctt gaacctgcaa
     2761 gacgagggtc gagtttggac catgctcgcg gggccggatc aggtcgcggt caccgtaccg
     2821 ccgttgctcg atcgaaccat cgactacctg cgctcgaagt cggtcctcga agagagcgag
     2881 aacgtgcgcg tagcgctggt cgcgcccacg ggcgaacctc gtttgctggg ggacatggcg
     2941 gaggccattc gcaagacgat tcgcttcaac ggcaagtctg cgctcgataa cttccccgac
     3001 aacttcaagc tgatcgacat cgaaacggtc tacggcgacg ccgaggccgc caaggatcag
     3061 tccggggtcg tgagcgagct gctcggtttc aagccgcacg tcatcctggc gatggctacc
     3121 aacgaatttc tgtccacggt catccccggc ctcgaagggc aatggtcgag cgcggccgcg
     3181 ggtcaggccc gcccgtttta cgtgctctcg ccctaccact tggggaacgt cgccatgccg
     3241 acggcgctgg gcgcggagag cacggtgcgc acgcggatgg tgggcgtggg ctacgcggcg
     3301 gccgaggacc gtagcgtata tcaggactac atcttgcgcc tagatcaggc ctatcccgaa
     3361 attcagaagg attcctcgcg ccttggcctg gagaacttct atgacgcgcc ctactacttg
     3421 atctacgccg ccgccgcggc tggcaacccg ccgcagctca cgggggactt gctcgccaaa
     3481 ggcatgaccc gactgatcaa gggcaacacc agctacgaaa tcggccgtga tgacatggtc
     3541 gaggcgttcg gtttcctgca ggccaacgcc aagaacacca tcaccctgaa gggcacgctc
     3601 gggccagcca acttcgacga gagcacgggg gctcgggcgg acggtggcag cgtattttgc
     3661 atggatacga gcaacaagtt cgcgccggac atcctgcgct ggaatagcgc gagcgagatg
     3721 ttcgtagacg ccgcaactcc gattggtacg gcttgcattc caggtttctg atgtctcgtt
     3781 ggttcgcacc gttcgcgtgc gggatcgcac tcaccctcgg cggttgctcg gccccggttt
     3841 cgttcgacga agagggcacg gtcgagcaga ccatcgtcaa tggcgaagct tcggggcccg
     3901 aggacgattt caccgtcgat ctttactcgg ggccgcccac ggcgcgggcg cattgctcag
     3961 gagcgctgat agctcccaat ctggtgatca ccgcgctcca ctgcgtggcg tacatctcgg
     4021 acgcgccgtt tgcctgtcag aacgacggta cactgtcgac tccacccccg ggcggctact
     4081 tgggcgcgac ggatgacccc aagacgattg acgtgcatct tggcgccgcg cacgacgagg
     4141 tccccgaagc cggcgtgttg cgcgtcttcg gcaccggatc gactcacatc tgtcggcacg
     4201 acttggcgtt gctgctgctc gatcgggagc tggatgcacc gatcgcgcgg ttgcgcttcg
     4261 ggtcgcccat cgagcgcggt cgcaggttac gcctgatcgg cttcggcgac tacggtgtat
     4321 cgggggcgcc gatcactcgg catgttcgag ccggcgcccg cgtgaccgac gtgggtcaga
     4381 cggacagcac ggccgccgtg ggcaccgctg cacccgagac cttcgtcgta accgaaggcg
     4441 cttgccaggg ggacagcggc gggcctgcca tgaccgagga taccggcgcg attgcgggcg
     4501 tgtactcgat tagtgcggcg gccacctgca cggctacagg ggtccgcaac gtgtacaccg
     4561 ggttgtaccc cttcgaagac ttcattcgtc aggcgttcga agtcgcgggc cacgagccgc
     4621 tgctcgaagg gcagggtgaa ggcggtgcgg gtggcagcac cgggacgggt gcttcgaaca
     4681 gcggcggggc gagcacctct ggtggcggag tgacgacggg aggtaccacg acccactctg
     4741 cgggtaccag cggcgaggag cccgagccgg aaccggagac ccccggcagc ggctcgcgtc
     4801 gtgatccaag ttgtgcatgt cgcgtgactc ccgcgggcgg cgccggcccc cagccgagcg
     4861 cctgggcctg gcccgtggcg ctggccgtgg cgggtttgct ggggcgccgt gcatctcgca
     4921 accgcacgcg ctgagttggc tcgaatcgag tcacccgttc gagttgtgca ggcggttgcg
     4981 caactcgggt gcaagatgcg cagttaaccc tgaaggttta ctggcggcgc tgaaacggac
     5041 gagc
//
LOCUS       DASTKU010000819         7299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1054743, whole genome shotgun sequence.
ACCESSION   DASTKU010000819 DASTKU010000000
VERSION     DASTKU010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054743"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VFQ61_28595"
     CDS             <1..844
                     /locus_tag="VFQ61_28595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958500.1"
                     /translation="TLKGSLVLNGPGHASAQLSIASPIEFKTREDPLQFRLEAGLEVL
                     KVEADAAQKSASLAANLGEISMNTGFGNFVSGIFGLSLKEGSPPQPGVDMTIPGLRGT
                     LVFDGSRDVVSAQGLDLAGAPALAVQNGNTLLSLRTSDAQQGPIAATFSGLPDDTLEL
                     GLPAGLQVELQYGMEPVASLIKGPANYLARDTLKTTAAPNTTVRLFQETGWDNLALTG
                     NQLGRLLRVSAGTIGMSSSIWPNDAVSAQADQCLTRQESSVGIAAENRHDLLSTLSVA
                     ACTP"
     gene            1036..2409
                     /locus_tag="VFQ61_28600"
     CDS             1036..2409
                     /locus_tag="VFQ61_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HET9958501.1"
                     /translation="MAAVAAGLVIACSNGGGTSGGISNGGSSFPQGGATHVGGAVQTG
                     GAVQTGGANTGGSAVAGAMGGANSFGGAAPLGGSNGGSMAGGKTGDGGGSGGGPAVTG
                     GRMGSADAGATQGGTESSGGPSGGNAGAGGGPLSTVGWVLSYFGPEQSVAADSLHLAY
                     STDGLRWTRLGSGGPAYQLTGMGTNHIRDPFILRTRDGSFVYLATDWTLSNNDNNYWN
                     NPSSKILVAESKDLIAFSNPRLLTLTPLKGPGGKPMHAWAPEAYYDAATEEYAIVWSG
                     NDSTDRNRIYVSYTRDFRTLVDPEPKVLFDPGYSVIDGTRVTSNGSSYLFFKDETDNN
                     GGAVTGSGKDIQIARAASEALVPGGFTRWDAGYIPRGTNQGTRQATEGPFVIKDPRRE
                     LWYLYADFYTQGGKFGCWSTPDLDAKPSAWTRLSESEYSLPAGVRHANTVRVTQAELD
                     ALKAHYK"
     gene            2493..4172
                     /locus_tag="VFQ61_28605"
     CDS             2493..4172
                     /locus_tag="VFQ61_28605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958502.1"
                     /translation="MWSATLACESRRETNSGEVGGGSTSTRETSGGRVGSAGQGGHVN
                     TGTETSLGRSGGGAFGGSTAADGTFGGAAGSWIDSEAGAQGGNGGATAEDPMVGGAGL
                     GGQPQSGGMGGASTHLSTNGGSAQGGSTQGGSNPAGGGPSSGGTSGAGGASDAALYVD
                     ANQLDLRVKLGSSNLPNGVRLEFDVTGDQNAELSGEFAAKDPGPALRIYVENNHRFEL
                     EACRQVDFEPATGTLRIRVPRAYLRNAPNPGVRAEFGSTRLGWLKSKSVEVLPSFEGQ
                     PLECGKDWGTVIGSRRFKQYLGINSFATFSRGCGLDEKGEVACLRYISEPPEMPASGP
                     FVEIGLNRENRLCGRRDSGTVDCWPLATSDGKFPRSFASPPNGKYRKLSEYGSCAIAE
                     DASLRCFTESEEVSAFTAGAGTFVDVAGSASQGCAVRTTGELQCWGFSDTEFAPAPKV
                     ALLAGRYRQVAVANAVVCALNVDGGVDCVHGYRSSILSLAGNDFSSVSGFQAACAVEK
                     SGNATCFGLTLPFPPLGFRTLVAGEVLTPSCGLLSGGQIGCWSATGGLSIP"
     gene            complement(4207..>7299)
                     /locus_tag="VFQ61_28610"
     CDS             complement(4207..>7299)
                     /locus_tag="VFQ61_28610"
                     /inference="COORDINATES: protein motif:HMM:NF024904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9958503.1"
                     /translation="FGLDHERLRDAGQSLLQGGGDVGESLFDAGAGGHGVGRVLSRLR
                     EESERLFKPRGGKQEIPQLLESFKAARERLHNATHVPEKYLEQQRELVRHREEHQALA
                     RELSEHRHERERSRLLQATLKGIAKRAKLLGDLDAIGAVPELPPDFGQRRDRVQGALS
                     THRANVERAERETARLTQRAAELRLPSSLLTVDEDTIASVREGIGSTKKALLDLPLRE
                     ATLIERRAEAQAVERRIGLDSERVASEALKARRPEEAKFRRLLAERGALAERLRLSRE
                     RLAQLELDCSTRKAKLATLPQSVKIEALERAVALARHVGDVEGALAQTRRERAELELV
                     ARAELATLAPFNGSLTELVTLRVPATETVQVFEQRFAKIEEERRRILAELERCRERAA
                     ELSRELAAEEQAGAVPSESELERSRRTRDERFDELLRAWPESPAKGRSLFDAARLREY
                     RSSVGAADALADRLRREAARVAENARRSAELTQLGQEQQRLGELTEALSSERDALERE
                     WASSWAAAGFQPVRPSEMRGWLARRERAVAHVTKEAAVVERERSLEAAVRELSQTLRD
                     ALDDDQLPVAVRAGQAAEKLDRERRLENERRALELQIAELEVRLQTARQELQASETEA
                     QQRELELAAVIQSLGFESGIAPEQVEARLEALNELVATRQQTSELERRIAGMRRDIAA
                     FEAQVQALVQSHAPDLAELPSAHAAAEIYARFERGRRDAENRARIEEDLRVQTAELEE
                     ERAALTRAESEAVQLLAMAGARDAIELPGIELRVAKARELRAELDGLEASLGATAGPR
                     GLNVLLEEAAATDPARLSARLEELERLIEELEERHSESIRAQQRVQAGLELYSDASAA
                     EAAAEERLLASALVVRAERWAKFKLAEVLLAKEIERYREQNQGPVLRRAAELFVKLTH
                     GQFRGLRLGREERTLLTVRANEQEVGVEGLNEAARYHLYLALRLASLERYLEHAEPLP
                     LVLDDVLIHFDDDGARAALGVLAELSDRIQVLLFTHHRHNLALAESTSASGRLFIHEL
                     "
BASE COUNT         1239 a         2275 c         2458 g         1327 t
ORIGIN      
        1 aactctcaag gggagtctag tgctgaacgg acctggccac gcttccgccc agctttcgat
       61 tgctagtccc atcgagttca aaacgcgtga ggaccctctg cagtttcggc tcgaagcggg
      121 gctagaggtc ctgaaggtgg aggcggacgc cgcccagaag agtgcgtcgt tggcagccaa
      181 cttgggcgag atcagcatga atacggggtt cggcaacttc gtttcgggca tctttgggtt
      241 gtctctgaag gaaggttctc cccctcagcc tggagtggac atgacgatcc ccggactgcg
      301 tggaacgttg gtgttcgacg gcagccggga cgtggtgtcg gcgcaaggtc tcgatttggc
      361 aggcgcgcca gcgctggccg ttcaaaacgg aaacacgtta ctttcgctcc gcaccagcga
      421 cgctcaacaa ggcccgatcg ccgcgacctt ctcggggctg cccgatgata cgctcgagct
      481 cggcttgcca gcgggtttgc aggtcgagct tcaatacggc atggagccgg tcgcatcgct
      541 aatcaaaggc ccggcgaact atttggcgcg ggacacactc aagacgaccg ccgcgccgaa
      601 caccacggtg aggctgtttc aagaaacggg ttgggacaat ctcgcgctga ctggcaatca
      661 gctgggtcgg ctgcttcggg tgagcgcggg cacgattggt atgagcagca gcatctggcc
      721 gaatgacgcg gtgtccgccc aggccgatca atgtctgact cgccaggaga gttcggtggg
      781 gatcgcggcc gaaaaccggc atgacctctt gagcactctg tccgtcgccg cttgtacgcc
      841 ttgagcgtcg ccggagcttg gacgacgagt ctcaggcgcc acaattttcc gatacggaga
      901 caaacaggca acgctcattc ttcgaaacgc ggatcggcag agcgtgtcgc tgacccgcag
      961 gcgcgcgggt gcggtagggt cgtcggaatg cagtttactt cgcataggca acgttcgaga
     1021 ggaatagctc cgttcgtggc cgcggtcgcg gccgggctgg tgatcgcgtg cagcaacggc
     1081 ggcgggacta gcggggggat atcgaatgga ggctcgtcgt ttcctcaagg cggggccact
     1141 cacgtgggcg gagcagtgca gacgggcgga gcagtgcaga cgggcggagc gaacactggt
     1201 ggaagcgcag tagcgggcgc gatgggcggc gccaacagct tcggcggagc agctccgctg
     1261 ggcggtagca acggcggcag tatggccggc ggcaaaacgg gtgacggcgg aggaagcggt
     1321 ggcgggcctg cagtcaccgg tggccgtatg ggctccgccg acgcgggcgc aacccagggt
     1381 ggcaccgaaa gctccggcgg cccgagcggg ggaaacgctg gcgcgggcgg aggtccgctc
     1441 agcacggtcg gctgggtttt gagctacttc ggccccgaac agtccgtagc ggctgactca
     1501 cttcacctcg cgtactcgac ggatggcttg cgctggacgc gcttgggctc gggggggcct
     1561 gcctatcaat tgaccgggat gggcacgaat cacattcgtg atccgttcat tctccgcacc
     1621 cgggacggca gcttcgtgta tctcgcgacc gattggacat tgtccaataa cgataacaat
     1681 tactggaaca atccgagttc caagatcctg gtcgcagaat cgaaggatct gattgcgttc
     1741 agcaacccgc gcctgctcac actcacgccg ctcaaagggc ccggcggcaa gcccatgcac
     1801 gcctgggcgc cggaagccta ctacgacgcg gcgaccgagg agtacgcgat cgtgtggtcg
     1861 ggcaatgaca gcacggatcg caatcggatt tacgtgagct acacgagaga cttccgcacc
     1921 ctcgtggatc cggaacccaa ggtgcttttc gatccggggt atagcgtgat cgacggtacg
     1981 cgggtcacgt ccaacggttc ttcttatctc ttcttcaagg acgagacgga taacaacggc
     2041 ggcgccgtga cgggttcggg caaagacatc caaatcgcgc gtgccgcatc ggaagctctg
     2101 gttccgggcg ggttcacgcg ttgggacgcc ggatatatcc cgcgcggaac caaccagggc
     2161 acgcgccaag ccacggaggg ccccttcgtc atcaaagatc caaggcgcga actttggtat
     2221 ttgtatgctg acttctatac acaaggcggc aagttcggtt gctggagcac tccggatctc
     2281 gacgcgaagc cgagcgcgtg gacgcggttg tccgaatccg agtacagttt gccggcgggc
     2341 gtgcggcatg cgaacacggt ccgcgtcacg caggccgagc tggatgcgtt gaaagcgcac
     2401 tacaaatgac gggattttcg ttaactaggc gcggcctcga agggttcgca ggaagaagcg
     2461 cgctggcgcg gctggtttgt atctcgagtt tcgtgtggag tgccacgttg gcctgcgaga
     2521 gtcgccggga gacgaactcg ggggaagttg gagggggcag cacgagcacg cgagaaacga
     2581 gcgggggtcg cgtcggttcg gcgggccagg gtgggcacgt gaacacgggg accgaaacga
     2641 gtttgggaag gagcggcgga ggagcattcg gtggtagcac cgcggctgac ggcaccttcg
     2701 gcggggcggc gggctcgtgg atagactcag aagcaggcgc acaaggcggc aacgggggag
     2761 caacggctga ggatccgatg gtcggaggcg caggacttgg agggcagccc cagtcgggcg
     2821 gcatgggagg agcatccacg cacctatcca cgaacggcgg gagcgcacag ggggggagca
     2881 cacagggcgg gagcaatcca gcaggtggtg gcccctcgag cggtggcacc tcgggagccg
     2941 gcggcgcaag cgatgccgca ctgtatgtcg acgcgaacca gctcgatttg cgggtgaaac
     3001 tcggtagctc caacctcccg aacggggtgc ggctcgagtt cgatgtgact ggggaccaga
     3061 acgcggagct ctctggggag tttgccgcca aagaccccgg ccccgcgtta cggatttacg
     3121 tggagaacaa tcaccggttc gaactcgagg cctgccggca agtcgacttc gagccggcca
     3181 cgggaacgct gaggatccgt gtgccgcgcg cgtatctgcg caatgcaccg aatcccggtg
     3241 ttcgcgccga attcggcagc actcgcctgg gttggctcaa gtccaagagc gtggaagtgc
     3301 tgccttcgtt cgagggtcag ccgctcgagt gtggcaaaga ctggggaaca gtcatcggct
     3361 cccggcgttt caagcagtac ctcggaatca actccttcgc gaccttctcg cgaggctgcg
     3421 gactggacga gaagggcgag gtcgcgtgtt tgcgctacat cagcgagccc ccagaaatgc
     3481 ctgcgtcagg tccattcgta gagatcgggc taaatcgtga gaaccggcta tgtgggcgac
     3541 gggattcggg aaccgtggat tgctggccgc tcgccacgtc ggacgggaaa ttcccgcgct
     3601 cgttcgcgag ccctccgaat ggcaaatatc gaaagctgtc ggagtacggg agttgtgcca
     3661 ttgcggaaga cgcgagccta cgttgcttca ccgaaagtga ggaagtctcg gcgttcaccg
     3721 cgggtgccgg cactttcgtc gatgtcgcgg gcagcgctag tcaaggttgc gcagtccgca
     3781 cgacgggcga gctccagtgc tggggattct cggataccga atttgcgccc gcgccaaagg
     3841 tcgcattgct ggcggggcgt tatcggcagg ttgcggtggc caacgccgta gtgtgcgcgc
     3901 tcaacgtgga cggaggggta gactgcgtgc acggctatcg ctcctcaatt ctgtcgctcg
     3961 ccggcaacga cttcagctcc gtgagcggtt ttcaagccgc ctgcgccgtc gagaagagcg
     4021 ggaacgccac gtgttttggc ctgaccctcc cctttccgcc gctcgggttt cgcacgttgg
     4081 ttgcgggaga ggtgctcacg ccgtcttgtg gtttgctcag tggggggcaa atcggatgct
     4141 ggagtgcgac gggcgggctc tcgattccgt aaccgcgtcg ggagccgcgg cagacgaccc
     4201 cagggctcaa agctcgtgaa tgaacagccg ccccgaggcg ctggtgctct cggccaacgc
     4261 caggttgtgc cggtgatgcg tgaagagtag gacttgaatg cgatccgaga gctccgccag
     4321 aactccgagt gcagcacgcg cgccatcgtc gtcgaaatga atgagcacgt cgtcgagtac
     4381 tagcgggagc ggctcggcgt gttccaagta gcgttcgagg ctggccaagc ggagcgcgag
     4441 atagagatga tatcgcgccg cctcgttcaa gccttcgacg ccaacctctt gttcgttcgc
     4501 gcgtacggtc agcagggtgc gctcttctcg cccgagccgc agccctcgga attgcccgtg
     4561 ggtgagcttc acgaacagct cggcggcacg acgcagtacg gggccctgat tttgctcacg
     4621 atagcgttcg atctctttgg ccaagagcac ttcggcgagc ttgaactttg cccagcgttc
     4681 cgcgcgcacc acgagcgcgg aggccaatag tcgctcttcg gccgcagctt cggcggcgct
     4741 cgcgtccgaa tacaattcga gacctgcctg gactcgctgc tgggcacgaa tgctctcgga
     4801 gtggcgctct tcgagctcct cgatcaatcg ctcgagctcc tcgagccgcg cggagaggcg
     4861 cgccggatcg gtcgcggcgg cctcttcgag cagcacattc agtccgcgtg gaccagcggt
     4921 ggccccgagg ctcgcttcga ggccgtcgag ctccgcgcgc agttctcgtg ccttggcgac
     4981 acgcagctca atcccgggta gctcgatcgc atcacgtgcc ccagccatgg cgagcagctg
     5041 aaccgcttcg gattcagcgc gcgtcagcgc cgccctttcc tcttccagct cggcagtttg
     5101 aacgcgaaga tcttcttcga tgcgggcccg attctcggcg tctcgtcgcc ctcgttcgaa
     5161 gcgcgcgtag atctcggcgg cggcgtgagc actgggtagc tccgcgaggt cgggcgcatg
     5221 ggactgcacc agcgcttgga cttgcgcttc gaaagccgca atgtctcgcc tcataccggc
     5281 aatgcgccgc tcgagttcac tcgtctgctg ccgtgtcgcg acgagctcgt tcaaagcttc
     5341 gagtcgagct tccacttgct ccggagcgat cccggactcg aaaccaagcg actggatcac
     5401 ggccgcgagc tcgagttcac gctgctgcgc ctccgtctcg ctggcctgca gctcttgacg
     5461 cgcggtttgg agccgcactt cgagttctgc aatctgcagc tccaaggcgc gccgctcatt
     5521 ttccaaacgc cgctcgcggt cgagcttttc ggcggcctgt ccggcgcgca cggccaccgg
     5581 gagctggtca tcgtccagtg cgtcgcgcag ggtttgcgag agctcacgta cggcggcttc
     5641 gagtgaacgc tcgcgctcca cgaccgctgc ctccttcgtg acgtgcgcca ctgcgcgctc
     5701 ccgccgcgcg agccagccgc gcatctcgct cgggcgcacg ggctgaaaac ccgccgccgc
     5761 ccagctcgac gcccactcgc gttcgagcgc gtcgcgttcg ctcgagagag cttcggtgag
     5821 ctcgcccaag cgttgctgtt cttgccccag ttgcgtgagc tccgcgctgc gacgggcgtt
     5881 ctccgccacg cgcgcggctt cgcggcgtag ccgatcggcg agcgcatcgg ccgctcccac
     5941 cgagcttcgg tattctcgga gccgagcggc atcgaatagg gagcgtcctt tggcggggct
     6001 ttcaggccag gcgcgcagca gctcatcgaa ccgctcgtca cgcgtgcgcc gtgagcgttc
     6061 gagctcgctc tcgctcggca cggctccggc ttgctcttct gccgcgagct cccgggacag
     6121 ctccgcggcg cgttcccggc agcgttcgag ctccgccaag atgcgcctgc gctcctcctc
     6181 gattttggcg aagcgttgct cgaaaacttg taccgtctcc gtcgccggaa cccgcagtgt
     6241 cacgagttcg gtcaagctgc cgttgaatgg cgcgagggtg gcgagctccg cgcgcgccac
     6301 gagctcgagc tccgctcgtt cacgtcgcgt ctgcgccaag gcgccctcga catcccccac
     6361 atgccgcgcc aaagccaccg ctcgctcgag agcctcgatc ttgacggatt gaggcagggt
     6421 ggcgagcttg gctttgcgcg tggaacagtc gagttcgagc tgcgcgagcc gctctcggct
     6481 gagtcgtagg cgttctgcta gtgcgccgcg ctccgcgagt aagcgacgga atttcgcctc
     6541 ctcgggccgg cgcgctttga gagcttcgct cgcgacgcgc tccgagtcca aaccgatgcg
     6601 ccgttcgacc gcctgggcct ccgcccgtcg ctcgatcagg gtggcctcgc gcagcggcag
     6661 atcgaggagg gccttcttgg tgcttccgat cccctctcga acactcgcga tcgtgtcctc
     6721 gtcgacggta aggagtgaac tcggcagccg aagctccgcc gcgcgctggg tcagtctggc
     6781 cgtctctcgt tcggcgcgct ccacgttggc ccgatgggtg ctcagcgcgc cttgaacgcg
     6841 gtcccggcgc tgcccgaagt cggggggtaa ctcgggcacg gccccaatcg catccagatc
     6901 gccgagcagc ttggcgcgct tggcgatgcc cttgagagtg gcttgtagca gccggctgcg
     6961 ctcgcgctca tgacgatgtt cggagagctc ccgagcgagg gcctgatgct cctcgcgatg
     7021 acgcacgagc tctcgctgtt gctccaggta tttctccggg acgtgcgtgg cgttgtgcag
     7081 ccgttcgcgc gccgctttga agctctcgag cagctgagga atctcctgct tgccgccgcg
     7141 cggcttgaag agccgctcgc tctcctcacg cagccgcgag agcacgcgcc ccacgccgtg
     7201 cccgcctgca ccggcgtcga acaggctctc gcccacgtcc ccaccgcctt gcagcaagct
     7261 ctgacccgcg tcgcgcagac gttcgtgatc gaggccgaa
//
LOCUS       DASTKU010000820         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1725091, whole genome shotgun sequence.
ACCESSION   DASTKU010000820 DASTKU010000000
VERSION     DASTKU010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725091"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VFQ61_28615"
     CDS             <1..623
                     /locus_tag="VFQ61_28615"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET9958504.1"
                     /translation="LTSAASLRAHHARGAVSWDIVRGVTPGILLGTFGGTFLASRVPT
                     VPLALFFVSFMAYVALQMAMNVKPRPARQLPGAPGLFGVGSVIGLVSALVAIGGGSLS
                     VPFMTWCNVRMQNAIGTSAAIGLPISVAGAVGYFINGAGVVGLPPYSVGYLYVPAMCC
                     ISLVSITTAPLGAKWAHELPVATLKKIFAGVLLLLSAKMLHSVFVQ"
     gene            complement(686..1465)
                     /locus_tag="VFQ61_28620"
     CDS             complement(686..1465)
                     /locus_tag="VFQ61_28620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958505.1"
                     /translation="MVNPDDLLTALGRVVRDREEVERERVSTAASAPPLTPSINAARV
                     DTAHTHDSLVAAVLAELAKPAAPSKVDETPEVVPSTLAHSPSAPSVLASRRRLWVGLP
                     TLAAAAALLFVLGRGKEQQPADLPALTPYSAELQGGIAAVRGGAKSDAPLKLAPGTEL
                     SLFLRPDAPVSGNVVAAVVLFARDSSSASRKLAVDQTVAASGALRLSVDLPNELPAHG
                     DVCAVVAREQHLETGMSGVGASSGDGWQRFCWEFSTTARRQ"
     gene            complement(1646..2275)
                     /locus_tag="VFQ61_28625"
     CDS             complement(1646..2275)
                     /locus_tag="VFQ61_28625"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HET9958506.1"
                     /translation="MREFPQDSEAKLNKPLVQRALGGEAQAVRELIGLLHPVIKRRAL
                     ASLTRYGQGTQSEVEDVCQEVLIGLFREDRAALAAWDPERGLSLVQFVGMLAQRRVSS
                     YLRSRANKPSRRTPTEPESLERLVNQSASDQLDPHDPARETFHELADRLRATLSPLGL
                     EMFYRLYVWEHSTEQITAQLGLTAEAVYQWRSRLRKAALLALKELEHGQ"
     gene            2404..>2602
                     /locus_tag="VFQ61_28630"
     CDS             2404..>2602
                     /locus_tag="VFQ61_28630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958507.1"
                     /translation="MTSTRIEDSSTESRSSSLEGSPYPSRRMPRLSAGSTLGSGRFEI
                     RGPLGAGGMGVVYEAYDRLARQ"
BASE COUNT          444 a          821 c          854 g          483 t
ORIGIN      
        1 tgctcacctc ggcggcgagc ctgcgtgcgc accatgctcg cggcgcggtc agctgggaca
       61 tcgtgcgggg cgtcacgccg ggaattctgc tcggcacgtt tggtgggacg ttcctagcgt
      121 cgcgcgtccc caccgtgcca ctcgcgctgt tcttcgtgag tttcatggcc tacgtggcgt
      181 tgcagatggc gatgaacgtc aagccgcgac ccgcacgcca attgccgggc gccccgggat
      241 tgttcggagt tggttcggtg attgggctcg tctcggcgtt ggttgcgatc ggcggaggct
      301 cgctgtccgt gcctttcatg acgtggtgca acgtccgcat gcagaacgcg attggaactt
      361 cggcggccat cggtctgccg atatccgtag cgggagcggt gggctatttc atcaacggag
      421 cgggtgtagt cgggctgcca ccgtacagcg tgggttacct gtacgtgcca gccatgtgct
      481 gcatcagctt ggtgtcgatc acgaccgcgc ccctcggcgc gaagtgggcc cacgaacttc
      541 ccgttgcgac tttgaagaag atcttcgcgg gggttttgtt gctgctgtcg gcgaagatgc
      601 tgcactcggt gttcgttcag tgaccactgg cgcgtggagc tagctcgacc gatcgacgag
      661 accgagcgcg agttcgcggc gcaaattact gccggcgggc ggtcgtcgag aactcccagc
      721 agaagcgctg ccagccgtcg ccgctggacg cgccaacgcc ggacattccg gtctcgaggt
      781 gttgctcccg agcaacgacg gcgcacacgt ccccgtgcgc gggcagttcg ttcggcagat
      841 cgacgctgag acgcaacgcg ccggaagcgg cgaccgtctg gtccacggca agcttgcgcg
      901 aggcgctgga agagtcgcga gcaaacagca ctacggccgc aacgacgttg cccgagactg
      961 gggcgtcggg acgcagaaac aggctgagtt cggtacccgg cgccagcttc aatggggcgt
     1021 ccgacttcgc ccctccccgt acggccgcga tgcctccttg gagctctgca gaatagggcg
     1081 tcaacgccgg caagtctgca ggttgttgct ctttgccacg ccccaatacg aacagcagcg
     1141 ccgcagctgc cgccaacgtc ggtaacccga cccacaaccg cctgcgcgac gccagaacac
     1201 tcggagccga gggtgaatgc gccaacgtgc tcggcacgac ctcgggagtt tcatcgacct
     1261 tcgagggggc agcgggcttg gcaagctccg ccaacaccgc ggcgaccaag gagtcgtgag
     1321 tgtgagccgt atcgacgcgg gccgcgttga tcgatggcgt caacggcggc gctgaagcgg
     1381 ccgtggaaac gcgctctcgc tccacctctt cgcggtcgcg aaccacgcgc ccgagggcag
     1441 tgagtaggtc gtcgggattt accatggctg gcctcgccgc aggggtttgg gtggctcgac
     1501 tcgtatgccg cgtttcactg aatggttatt cgcaacaagg gtcccgcttt ctgacgtctc
     1561 acggatcttt gtgtgcagat tcccaggcag caagctcgcc gatgcgcgcc aaaccaagcc
     1621 ccgaaaggga acgccaaccg cagtttcact gcccatgttc gagctccttc aaggccaaaa
     1681 gggccgcttt tcggaggcgg ctccgccact ggtacacggc ttcggcggtg agcccgagct
     1741 gcgcggtgat ctgttccgtc gagtgttccc atacgtacag ccggtagaac atctccaggc
     1801 ccaggggcga aagcgtggcc ctcagccggt ccgcaagctc gtgaaacgtc tcgcgcgcgg
     1861 ggtcgtgagg gtccagctgg tcggatgcgg actgattgac gagccgctcc aaactctcgg
     1921 gttcggtggg ggtgcgcctt gacggcttat tggcgcgact gcgcaggtag gagctcacac
     1981 gtcgttgcgc gagcatgccg acgaactgaa ccagagacag tccgcgctcc gggtcccaag
     2041 ccgcgagtgc ggcccgatct tcgcgaaaca gcccaatcag gacctcttgg caaacgtcct
     2101 cgacctcgga ttgcgtgcct tgtccgtagc gagtcaaact ggccagagcc cgacgcttga
     2161 tgaccggatg cagtagtccg atcagctcac ggaccgcttg ggcctcgcca cccagcgccc
     2221 gctggacgag cggcttgttg agcttggcct ccgaatcttg ggggaactcc cgcacaaaac
     2281 tcccataaca tttcgggccc acggttgtca ggtttgtctg gcgagctgcg aacagactgg
     2341 tgtgtgacgg gagcacgcga gctgtgcgcc gtcactttgg ccccaatgac cagagctcca
     2401 gccatgacct ctactcgcat cgaagattcg tctacggaga gtcgctctag ttcactcgaa
     2461 ggcagccctt acccaagtcg acggatgcct cgcctgagcg ccggctcgac gttgggtagc
     2521 ggtcggttcg agatccgcgg gccactcggc gctggcggaa tgggggtcgt ctacgaggcc
     2581 tacgaccggt tggcgcgcca ga
//
LOCUS       DASTKU010000821        26595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_831444, whole genome shotgun sequence.
ACCESSION   DASTKU010000821 DASTKU010000000
VERSION     DASTKU010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 26595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26595
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831444"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFQ61_28635"
     CDS             complement(<1..217)
                     /locus_tag="VFQ61_28635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958508.1"
                     /translation="MFESSRQHRRAWLSASGMLAVLMLGGGCMRDPNAAGATAPAVGN
                     SAAGSAARRNLPAAALDLGVPYPAEPQG"
     gene            290..655
                     /locus_tag="VFQ61_28640"
     CDS             290..655
                     /locus_tag="VFQ61_28640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958509.1"
                     /translation="MATRAQQFRTETQRSAQNAKPRPIAKKAPSKKQLAVDEMLAERS
                     GTHTAERNFSLRVTENKAGTALEDSATTPSRRSTRGSANHGRPSAQLERRQKRATHAP
                     ETVSSRAAAQSVRVRGHSA"
     gene            1011..2636
                     /locus_tag="VFQ61_28645"
     CDS             1011..2636
                     /locus_tag="VFQ61_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine exchanger family transporter"
                     /protein_id="HET9958510.1"
                     /translation="MLEFLHKSPLLILFGVIATGYLVGKLRVFGFALGVAAVLFAGLL
                     LGALVPSIELPEFVPQFGLVLFVYTLGLASGPGFFSSLRLRGLRDNGLTLLVLTLSCG
                     LTLALSKVFHFGGAAAAGLFSGAITNTPALAAVVEALKSSSADAKLLAAPVIAYSVCY
                     PMGVFVPLIGVWLADRLFGVSFEREPISLSYARQMGERIVNRTVKIEHSPSACARELR
                     TTDKWTVNFGRIQRGAALSIVHDETVFHSGDLVTVIGREADVLAAAKEIGSIASEPID
                     LDRSQIDYRRMFLSNSDVAGRPLSELHLTQRFDAVVTRVRRGDVDLVPTDTFVLELGD
                     RVRVLAPKDRMSELEKLFGDSLRHVSEIDVITFSLGIALGLLLGSIPVPLPSGGTFKL
                     GVAGGPLIVGLILGRVGRTGPLLWISPFGANLTLRQLGLVLFLAGVGLRAGKSFGETL
                     AEGNVLPLMMSGALVTLTSAFGTLVIGHKVMRIPLNVLVGTLAGLHTQPAVLAFGLEK
                     SGKDLPNVGYTSVYPFATIAKIVLAQVIIQLLH"
     gene            2774..3799
                     /locus_tag="VFQ61_28650"
     CDS             2774..3799
                     /locus_tag="VFQ61_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9958511.1"
                     /translation="MKYKKLGAAGVKVSELCLGAMTFGEADENSFMHGVSSDEKTAFD
                     VMNRALELGVNFIDTADVYGQDGLSERVIGAWFERDKRRDEVVLATKFRFNMGTGPNR
                     SGASRYRIQRCAEASLRRLKTDRIDLYQIHMQDLDTPEEETLRALDDLVSQGKVLYIG
                     CSNYAAYRLQNALWLSRSQQRAAYVTLQAEYSLITRDIEREHIPLCRENGLGVLAWSP
                     LAGGFLSGKYQRNQPPPSGARLEKWKDRYSRFDNERNWRILEATREIGNEHGVSSATV
                     ALAWLLKKPQLCSAIFGARTVSQLEDNVRSTDLELGDGQMKRLDEASAFELGYPYAFM
                     GNVQGRW"
     gene            complement(3905..4594)
                     /locus_tag="VFQ61_28655"
     CDS             complement(3905..4594)
                     /locus_tag="VFQ61_28655"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET9958512.1"
                     /translation="MAAQSGTAAHASVIGRGTSIRGHVRGDGNLEVHGRVEGDVSVSG
                     DLALSDSAVVRGNLSGSELRVAGTVQGDIRGSEAVFIEATARVVGDISAPRIGIASGA
                     LVKGLVQTDGEGPLGGAQPAARRGAAAPAPQRSQPFAAARPAPARVAAPAPTREAPVA
                     APRTARIVIPERPQAHAAHGEPARASAPERSENTQEDSAKKERPAAPPPVVPALAKQT
                     RATRRNNKRGS"
     gene            complement(4600..4914)
                     /locus_tag="VFQ61_28660"
     CDS             complement(4600..4914)
                     /locus_tag="VFQ61_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET9958513.1"
                     /translation="MAGTVIAEGLSIEGELSSDEEVVVHGSVRGKLTTTEQVSVANGA
                     VVNADVTGSSIAIAGQVTGDVTGSERVDLQAGGRLVGDVKAARFTIADGASFKGNVDM
                     EM"
     gene            complement(4936..5268)
                     /locus_tag="VFQ61_28665"
     CDS             complement(4936..5268)
                     /locus_tag="VFQ61_28665"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET9958514.1"
                     /translation="MIGSSTQITGRVSGDGHVRVDGAVRGDIDVAGDAEIAAGGRVEG
                     NVQADAVDVGGTLLGDVTARAQIAIRAGAQVRGELKAAEIAIEPGARVALRLNTEVEL
                     DLGTSRRR"
     gene            complement(5572..6327)
                     /locus_tag="VFQ61_28670"
     CDS             complement(5572..6327)
                     /locus_tag="VFQ61_28670"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET9958515.1"
                     /translation="MRRTSENLGEASELAQPWPEGWTTESVIERLEPLTSEARRQRLL
                     QIIERRVSAVTVLMDAPHDPHNAAAVIRTCEAFGLPELHVVPRDERLLFARSVTRGAQ
                     RWVNVTVHSDPTVAVGRLHAQGFELVATDPDGELLPDELSRIPRLALLLGNEHSGLRP
                     ELLRAASRSVRIPMRGFTESLNVSVAAAVLLASATQSRPGDLSPEQRRIAYARGLYHS
                     VTSAREILRLTTPPVLERPLPNETRPAATFAAK"
     gene            6479..6805
                     /gene="trxA"
                     /locus_tag="VFQ61_28675"
     CDS             6479..6805
                     /gene="trxA"
                     /locus_tag="VFQ61_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9958516.1"
                     /translation="MASSNNVQVFNDLNFEQEVLSSGVPVLVDFTASWCGPCKLLSPI
                     VDQVADELVGQVKVGKLDIDDSPITAGKYGVRGVPTVMVFKNGERAAQHVGLTTRQKL
                     IDLVKV"
     gene            complement(10182..11129)
                     /locus_tag="VFQ61_28680"
     CDS             complement(10182..11129)
                     /locus_tag="VFQ61_28680"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9958517.1"
                     /translation="MRSSDSSRADRDAPAQTPRHETRRVLPARAMLYGLRLAVVVPAY
                     KEERLLGRMLRRLPAEIDTVWVIDDASPDATAQVASSLGDSRVRVLRHRENRGVGAAI
                     VTGYRAAQDDHDVLVVMAGDDQMHPGDLPALVAPIAAGLADYVKGNRFAHPEAYRMPW
                     LRRLGGEVLSFATRLSTGLRVTDCQCGYTALSARAARTLPLEQLWPRFGYPNDLLGML
                     ASRQLRVQQVPVLPVYADEQSGLRPYHVAQILGLIVRRHWIEMRVRREMPARSNPASA
                     RLGVGLRSQLRWDLDAGIESAAHVPVLDVTAPRIVDLPE"
     gene            11289..11849
                     /locus_tag="VFQ61_28685"
     CDS             11289..11849
                     /locus_tag="VFQ61_28685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958518.1"
                     /translation="MGCALLVLGGFAWSWLTYPSDRTPEGAYLRVVIAVNRDRPQDFF
                     AYLETPAQHACFTIADYRRRARELVLKHYPEPQRTQLAAQYRVEADAKDGADVLAAYA
                     RSRGFMARLRRDLSGIAKIERNGERATVETARGTRYPFRIRPENGIWGLTLFTAELIQ
                     EAEKAARDYRIIEKAAQDYERAKTGG"
     gene            complement(11929..12360)
                     /locus_tag="VFQ61_28690"
     CDS             complement(11929..12360)
                     /locus_tag="VFQ61_28690"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9958519.1"
                     /translation="MPIQSVTPEQAHELMQQGATYVDVRSEQEFEQGHPPGSLNIPIA
                     HQRAGGMAPNPEFLKVMQALFATDANLVLGCKAGGRSRRAAELLQQAGYVSLSDMSAG
                     WDGSRDAFGRPLPGWHHRSLPVETGQPEGQRYEDLKAKAGG"
     gene            12482..14197
                     /locus_tag="VFQ61_28695"
     CDS             12482..14197
                     /locus_tag="VFQ61_28695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET9958520.1"
                     /translation="MKTPHGVLGFWSKYPGSDGLISEHKAGRVRLVPLGGLGEIGMNC
                     LAVEQHDGILVVDCGAGFPEDDRGVDVIHPDFAWLVAQGERVKGVFLTHGHEDHIGGL
                     PYLLAELDVPVWGPPHALGLVRRRLAEHDFGPHEVDLREAHAGNVYEVGPFRVEPIRV
                     AHSIVEASALCIETAMGRLLHTGDFNFDPDPPDGEPTDELRLEALGDAGVQLMLSDST
                     NIDVPERAGSERSVAECVERLVSAAEQRVVVAMFASNIQRLITFGEIAVRTGRKLCLL
                     GRSLETQYAIATQIGRVHWRSDLLVAADRAQSLPRERLMVLAGGTQAERNSALKRLSV
                     GVHPQLKLEAGDSVLLSSRVIPGNERAVIGMVNDLLRQGLHVHTRITEPGIHTSGHAG
                     RSEQSRMLELVRPRCFVPVHGTLHHLLRHAELARSLGITETLVVENGTTFVCDGERVE
                     RDIEVAAGRIPIAYGGEPLEAGILQARTELARTGIVLISLALDTNNRLVAPPAVTSRG
                     VPCVDESPDALRALAAEAARSVTNYRDGRGLGLSEFVRRTVRRKLEDLSGTRPNIEVT
                     LLETH"
     gene            14607..15587
                     /locus_tag="VFQ61_28700"
     CDS             14607..15587
                     /locus_tag="VFQ61_28700"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9958521.1"
                     /translation="MAQLRSPDGKRVVLLQSEHLIGRGEQCELRLNASYVSAQHAVIR
                     WSGARWEVLDRGSRNGTRVNEESLQASRVYPLALGSKLRFGHRDECWVFENDDPPEPT
                     LFLLGTGQAVSGNSGVIGIPSNASAEGTLMRAPDGSWKLEFLDGVTRPLANGEEFEYR
                     GVKYRFCYPYCAVATASSAESAAEGILCFNVSANEEFVETWVEYVWGRVALGSRAHNY
                     LLLTLARARQADQRNGVPESECGWLDKEELAAGLCMTPQQVDGDVFRARRHLGHLTLR
                     EAATVVERRPRTRQLRLGFSRTRIGVTAQEGPTAATPKPPISSDVTRREL"
     gene            complement(15597..16301)
                     /locus_tag="VFQ61_28705"
     CDS             complement(15597..16301)
                     /locus_tag="VFQ61_28705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958522.1"
                     /translation="MTATKKAKKKQSAPKRERRERRFTAEPTYASRVTAFAGMGGALV
                     LGAGVYAQWIRDEALSYAPVLVASGAAVLAWALWKSGGPLANVRVGEAGVALEKDNEL
                     NRLLWCDIERIALDKSDLLIRGKNAQLRFPLDAHQRAAAWIVSEAGQRVPDVVALSRA
                     EIERLPQTKDSDGELVNIEDVQVAGRHCKATGKPISFEREARLCPTCGEAYLKDHVPK
                     KCLTCNAELGSRAREV"
     gene            16411..17742
                     /locus_tag="VFQ61_28710"
     CDS             16411..17742
                     /locus_tag="VFQ61_28710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958523.1"
                     /translation="MGGAKRWPILILPSLTLGGAATLVGWGSLNREECSARVWGGPTD
                     GLAVFSGWVESCVNGPTLAGGQLKLEARARPGAQLAGTEVTLDDEGRAEFHLDFGDQP
                     PGEFYLRVEHAGRLLAEGRVALSRARFFGAAGERGGYLTSRSAGALEVRGAPERGALA
                     VPFDAPLRLEVRAEGAPLAGVDLELRAEGARLSTNHLKTNGRGEAQVIVTPQEHTVFF
                     TARATTIDAGTGESRMGELRSSVPVVPGACVVERGEEHRFLVKSPVERDAVFVTLIDD
                     KGRLAGSAVRLRPNGVGQALGTWDVSEPWASQAKWAVCSSDPRGSGAALVGWPLAIGV
                     DGPAHTRDVADVLLLDGEPARNARNALRRRWVHRLALMWGALGAGLSVAWVWVSSNRE
                     RRRIERELTGVLDARGVEQLTQTQAGLRLWVLCLIALGFLGLVALLALRLR"
     gene            17818..18714
                     /locus_tag="VFQ61_28715"
     CDS             17818..18714
                     /locus_tag="VFQ61_28715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958524.1"
                     /translation="MSELKLTRRVVLSVMGVEGGLLAASAVVGTSAVVATSAEARPPT
                     PAFRLDLETETGQKLRRYSHAGSWFVLGTPGERYAIRVTNPTAERVEAVVSVDGRDAI
                     SGRRGNYFTERGYIVPAYGSVLLEGFRSSLEEVRTFRFAPASESYSARMGTPENVGII
                     GVAIFKERRRVRRAPAPIAKADEGLTERREAPAGKRAAGSSSPAPAPAPASRSKGRRE
                     SAGASNIGTEYGEARESQVVEVPFVRRAERPDVVLTVRYDDADGLTARGIELYPVPRE
                     PVARPPAPPQAFPGSRFAPPPP"
     gene            19020..20258
                     /locus_tag="VFQ61_28720"
     CDS             19020..20258
                     /locus_tag="VFQ61_28720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9958525.1"
                     /translation="MKCWFHPIGVSTIALCTVSCSQEAEKPTTSSGVAASGNVSAAGN
                     PGLGANAGAGGGPAIATGGAPLTIGSSGGATLVPSIQGGNNTGGGTSAPETCAATYAD
                     TGLEPVYLGFAFDVSGSMGKGDEPYHDVELKWKPVVSATKAFFADPSSSKISASLSFF
                     PAKNDKCDASSYAEPDVPMTALPSDAFASAIDAITPKSADEWRGGTPTLAVISGVSGF
                     LRSFRANLGAGKFALVLVSDGYPQGCDHEEDEIETVAEEVARVATEFPTYVIGVSNPA
                     GGPDTVSNLNRVAEAGGTEKAFLIKTGDPEQTSRDFKMVINAIRGRTLSCQATIPKPP
                     DGAVLDTSKVNVSYTKAGELRPLAYDPACTEAGAWRFDDPTQPTRIELCDAACGDVQA
                     SADVKLSVEFGCETRPVIPR"
     gene            complement(20320..21633)
                     /locus_tag="VFQ61_28725"
     CDS             complement(20320..21633)
                     /locus_tag="VFQ61_28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HET9958526.1"
                     /translation="MQAHQAPLDAARDLLRFIQASPTPCHAVAEVERRLAQVGFRKFQ
                     EQDGWELQPGTRGYVTRAGSIIGFIAGDKAPAESGFVILGAHTDSPNLRVRPRPDVEN
                     LGYRELGVEVYGGVLLSTWMDRDLGLAGRVVTLDGVQHLLNVERPLFRIPNLAIHLNR
                     DVNKEGLQLNAQQHLIPVTGLESKTAQSLNEFVASSLATDAAPGVTARDIGGYELCLY
                     DLQAGSLGGGADEFLFSARLDNLASCHAACSALVRSGQGVDFTRLIALYDHEEVGSQS
                     SVGARSQFIDSVLLRLVGAFGEWRGDALPRALARSLLVSADMAHAVHPNYADKHDKQH
                     RPVLGAGPVIKVNSNQSYATGADGAAAFVRACRAAEVEPQYFVARNDMPCGSTIGPIL
                     SSRLGVRGVDVGNPMLSMHSCREMAATADVEPMIRVFERLFSAPR"
     gene            21657..22718
                     /locus_tag="VFQ61_28730"
     CDS             21657..22718
                     /locus_tag="VFQ61_28730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958527.1"
                     /translation="MLSPDVRVEGFSAEEWQRLGQIFRGPSPEFPHAEPRERTNGSAT
                     DRSADVGVGTQSRRGSGGLIALTDAGRLRKLLSTQRGRLDPRGYAGPLDLADLCARHG
                     ASWAVEVTHGTLERLVDRFAERLRPADTYLSQLLEFLLVLRELESEGDLRVFPWPVAD
                     WPIPTERAVVRAMEALCPVGRVALLGVFAQGELYTAAAFRRGASGIDAIVGPSELRAR
                     MGLLSGDWQRDYRFLASACETLLGPLALGCFGELFTFQTLAKDPAPGAWTSAVASREI
                     VLSPVSPALALPLGLDAGRALWAQVRGLAERFGGSGWLGQASKRAPFDRLPAFESDVV
                     AWLGFDPLRLLSRWMSARD"
     gene            22721..24343
                     /locus_tag="VFQ61_28735"
     CDS             22721..24343
                     /locus_tag="VFQ61_28735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9958528.1"
                     /translation="MRNVAVAGRIQHRLALAIVLTALIPVLVAMWMAQNTVRQTAARF
                     YIPEVGMHLDRSLDLYKELARSVKALMREEAAAIAERHRLREAVRLRDSAAMQQELTR
                     AFGEHPSLVSLSIRDADGNELSRVQRPKPLDPAREKDLEVERSLREADDGEMLMAVFA
                     TPRDRFDELSEMNQFVDTYRKLEHRREADERSYVLAFAVLLGITIVAAIGVGVLLARS
                     VSGRIGELAIATKWVAEGDLTIRVPERGKDELSDLARAFNRMLGEVTESRSRIEYLQR
                     IGAWQEMARRLAHEIKNPLTPIQLAVQEIHRRYTGSDPAFRRLLDTTLEIVEDEVGTL
                     RRLVSEFSNFARLPQAKLEPADLADFLRELATQLRIEDRDADLPEDLRACLQGTHIHL
                     DFEVPEEAAEVLMDRQMLRRVLMNLIQNAAQALRDTREDAARIRVRLARDGDFYVVDV
                     EDTGPGIPETLGERIFDPYVTSKPDGTGLGLAIVKKITMEHGGRISASTSDLGGARLR
                     VRLPALGTRAAALASATSLERSGEFARAALMR"
     gene            24429..25013
                     /locus_tag="VFQ61_28740"
     CDS             24429..25013
                     /locus_tag="VFQ61_28740"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9958529.1"
                     /translation="MPSSSRSPRSQSPFTSVIWVSLIAIAAGLFVLPRLQPGNALTGL
                     SGGTEPAPDFTLPVIHGGELDSRLRLSALRGKLVLLDFWASWCQPCREQAKVLRAVAG
                     LPENRDLEIVGINVSDQPDAARRYLKADAPPWVVVEDPEGVANSAYSVETLPTLVAVN
                     ADGKLFTVRRRFVPERELNAILEGMRSQARSQRH"
     gene            complement(25066..>26595)
                     /locus_tag="VFQ61_28745"
     CDS             complement(25066..>26595)
                     /locus_tag="VFQ61_28745"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9958530.1"
                     /translation="GVVYEVEHVHTGEQLALKLLHSTWPGDGAPSAPLSQPPSFDASL
                     IARFQREARAAAKIRSEHVVRVTDAGLAPELGNAPYFVMEQLDGLDLERLVERRGALS
                     PSAVVSVLAQVARALDRAHSLGIVHRDLKPENLYLHRKEDGAVIVKILDFGISRFATD
                     QGDPQNLFVTKDGTLLGTPFYMAPEQARGRVHETGPAADVWAIGIIALRLLTARAYWS
                     ANTLAELMVQILSEPMPPPSVRFPELGTRLDAWFFRSCDREPSRRFASAGEQVRALSQ
                     ALSVPIADEQLTQSLVRLMGNELELVVRQSEVAPIADLSRTATYRQESGARALPSSSS
                     NPTPNSLSPSAPATLVRSGERAKSPALRVAVIAAVAVLSAGLAVSWWLGRTRANAPLE
                     ARPAAIPQASPGLPVAPNPTRAEPAVEPLPDNAPAPEQNSAASNAPSELEPRTHSKPL
                     LQSPTESSAAGAKAKPGAHAPVFGKPAPTSAPAKSSSVPATSRTSTALKPPKPYDPLA
                     P"
BASE COUNT         4541 a         8244 c         9074 g         4736 t
ORIGIN      
        1 gtccctgcgg ctccgccggg tagggcaccc ccagatccaa agctgcggcg ggcagattgc
       61 gtcgagccgc gctcccggca gcgctgttcc ctactgccgg cgccgtcgcg ccagcggcgt
      121 tcggatcccg catgcacccc ccgcccaaca tcaacacagc gagcatccca ctcgcagaga
      181 gccaggcgcg gcggtgctgt ctcgaactct cgaacatgcc ccctcctatc atcgcggcgg
      241 gcaggcgtct tgaccgctta ctggctcgaa agttgctcag tcaaagcgca tggccacccg
      301 agcacagcaa tttcgcaccg aaactcaacg cagcgctcag aatgccaaac ctcgcccaat
      361 cgcgaagaaa gccccctcga agaagcagct cgcggtagac gagatgctgg ccgagcgcag
      421 cggtacacat accgccgaac gcaatttcag cttacgagtc acagaaaata aggctggaac
      481 ggcactcgaa gattcggcaa cgacgccgtc gagacgttcg acgcgcggct cggccaatca
      541 cggacgccct tcggcgcagc tggagcggcg ccagaagcgc gcaacccatg ccccagagac
      601 cgtgagtagc cgcgccgccg cccagagcgt cagggtgcgc gggcactcgg catgattggc
      661 gtgttccgca ggggcgccac gctcccgcag cgcccaattc ggaggggcta gctccgagag
      721 agagtgggtg taaccccggc gtcggctacc gacgagacac caagtcgaga ttggcgccac
      781 acgaatcatg gcggtcgcca gcagtccgag ctaacgcggt ctgggccgta gtcgccgcgg
      841 gaacgcacca cggttcgatc cggccgctca atctcctcgg acctccaata aacggaggcc
      901 cacttccaga gcccgactca tcgaggattt tgggctcgtg gggtttaggg atagattgag
      961 cggcggatgg ccgtgcgggg cgggtccccg cggccacggc cgagactgcc atgctcgaat
     1021 ttctgcataa aagcccgctg ctgatcctgt tcggggtgat cgcgacgggt tatctggtcg
     1081 gcaaactgcg ggtgttcggc tttgctttgg gcgttgcggc cgtgctcttc gcagggttgc
     1141 tgctgggcgc gctggtaccg agcatcgaac tgccggaatt cgtgccgcag ttcgggttgg
     1201 tgttgttcgt atacacactc ggattggcga gcggacccgg ctttttctcg tcccttcgct
     1261 tgaggggcct gcgggacaac gggctcactt tgctggtgct cacgctgtcc tgcggtttga
     1321 ccttggcgtt gtccaaggtg ttccacttcg gaggggccgc ggcagcgggc ctgttctccg
     1381 gtgccattac gaacactccg gcgctggcgg cggtggtcga ggccttgaag agctcctcgg
     1441 cagacgcgaa gttgctggcc gcgcccgtga ttgcgtattc ggtgtgttac ccgatgggcg
     1501 tcttcgtgcc cttgattggg gtgtggctcg cggaccgcct gttcggggtg agcttcgagc
     1561 gcgaaccgat ttcgctctct tacgcgcgcc aaatgggcga gcgcatcgtc aatcggacgg
     1621 tgaagatcga acacagcccg agtgcctgcg cgcgcgagct gcgcacgact gacaagtgga
     1681 ccgtgaactt cggacgcatc cagcgcggag cggccctgag catcgttcac gacgaaaccg
     1741 tgttccattc cggtgacctg gtgacggtga tcgggcgcga ggcggatgtg ctggcggcgg
     1801 cgaaggagat cggcagcatc gcgagcgagc ccattgattt ggatcgctcc caaatcgact
     1861 accgtcgcat gtttttgtcg aattccgacg tagccggccg gcccctgagc gaattgcacc
     1921 tcactcagcg cttcgatgcg gtcgtgacgc gggtacgccg tggcgacgta gatttggtgc
     1981 ccacggacac gttcgtactc gaactcggag accgcgtccg tgtgctggca cccaaagatc
     2041 gcatgagcga actcgagaag ttgttcgggg attcgcttag acacgtctcg gaaatagacg
     2101 tgatcacctt tagcctcggc attgcactcg gtctgctcct gggctccatc ccggtacctc
     2161 tgccttcggg ggggacgttc aagctgggag tagcgggcgg ccctttgatt gtcggcctga
     2221 ttctcggccg cgttgggcga actggtccat tactgtggat atccccgttt ggcgcgaacc
     2281 tgactttgcg gcagcttgga ctggtgttgt ttctggccgg cgttgggctg cgcgcgggga
     2341 aatcgtttgg cgagacattg gctgaaggca acgtcctgcc gttgatgatg tcgggggcgc
     2401 tggtcacgct gacgagtgct tttggcacct tggtgattgg gcacaaggtg atgcgcattc
     2461 cgctcaatgt tttggtcggc actctggccg ggctccatac tcaacctgcc gtattggcgt
     2521 tcgggctcga gaagagcgga aaagacttac ccaacgtggg ttacacgagc gtctacccgt
     2581 tcgcgacgat cgcaaagatc gtgctcgctc aagtcatcat ccaactccta cactgagcca
     2641 tggacggcgc gtcccttgga cgacgctcgc gtcgccggtg ggtgctttga cctgctgttg
     2701 tcgagcgtca gcgagagttt tgatttcgcg cttcgggctg cgcaatccgg gctaatacaa
     2761 gggcggagga cccatgaagt acaagaagct cggtgccgct ggcgtcaagg tctccgaatt
     2821 gtgcctcggt gcgatgacgt tcggcgaagc cgacgagaac tcgttcatgc acggcgtgag
     2881 cagtgacgag aagacggcgt tcgacgtaat gaatcgcgcg ctcgagctcg gcgtcaactt
     2941 catcgacacc gcggacgtgt acgggcaaga cgggctgagc gagcgcgtga tcggcgcttg
     3001 gttcgagcgc gacaagcggc gcgacgaggt ggtgctcgcc accaagtttc gcttcaacat
     3061 gggcaccgga cccaatcgga gcggagcttc gcgttatcgg attcaacgct gcgcggaagc
     3121 cagcctgcgc cgcctgaaga cggaccgcat cgatttgtac caaatccata tgcaggattt
     3181 ggacacgccc gaagaagaga cgctgcgcgc actcgacgac ttggtgagtc agggcaaggt
     3241 cctgtacatc ggctgttcga actacgctgc ttatcggttg cagaacgcgc tgtggttgag
     3301 tcgcagccaa cagcgcgcgg cctatgtcac gctgcaggcc gagtacagcc tgattacgcg
     3361 cgacatcgag cgcgagcaca tcccgctgtg ccgcgagaac ggcctgggag tgctggcgtg
     3421 gtcaccgctc gcgggcggct tcttgagcgg caagtaccag cgcaatcagc cgccgccgag
     3481 cggcgctcgg ctcgagaagt ggaaggaccg ttactcgcgc ttcgataacg agcgcaactg
     3541 gcgcatcctc gaagcgacgc gcgaaatcgg gaacgaacac ggagtgagct cggcgacggt
     3601 ggcgctggcg tggctactca agaaaccgca gctttgttcc gcgatcttcg gcgctcgcac
     3661 cgtgagccag ctggaggaca acgttcgcag caccgatttg gagctcggcg acggtcagat
     3721 gaagcggctg gatgaagcgt ccgctttcga gctcggttac ccctatgctt tcatgggtaa
     3781 cgtgcagggg cgctggtaac gagaacgcgc gtctcagcgc cggcgtcggc cggcgctgag
     3841 atgcgagccc gggggcggct tcgaccgccg cgcgaaagca ggcgcagcag cgcctgcgag
     3901 tcggtcagct gccgcgtttg ttgttgcgac gggtagcgcg ggtttgcttc gcgagcgccg
     3961 gcacgacggg cggaggtgca gccgggcgct ctttcttcgc tgaatcttct tgggtgttct
     4021 cgctgcgctc gggtgccgag gcgcgcgcgg gctcgccgtg cgcagcgtgg gcttgcgggc
     4081 gctccgggat cacgatgcgc gcggtgcgcg gcgcggcaac cggtgcttct cgcgtgggtg
     4141 ccggggccgc aacgcgcgca ggcgcgggac gagccgccgc gaaaggttgg gaacgttgcg
     4201 gggcgggggc cgccgcgccc cgacgggccg cgggttgagc tccgccgagc ggtccctcgc
     4261 cgtcggtctg cacgaggccc ttcaccaacg cgccagacgc gataccgatg cggggagccg
     4321 aaatgtcccc cactacgcgg gcggtggctt cgatgaacac ggcttcgctg ccacgtatgt
     4381 caccttgtac ggtgcccgcg acgcgcagct cggagccgct caggtttccg cgcacgaccg
     4441 cgctgtccga gagagcaagg tcaccgctga cactgacgtc gccttcgaca cggccatgga
     4501 cctcgaggtt accgtcgccg cgcacgtggc cgcggatcga ggtgccgcga ccaatcaccg
     4561 acgcatgcgc tgccgtacca gattgggctg ccatgccgct cacatctcca tgtcgacgtt
     4621 gcccttgaac gaggcgccgt cggcgatggt gaagcgggca gctttgacgt cgccgaccag
     4681 gcgaccccct gcctgcaaat ccacgcgctc actgcccgtc acgtcgcccg tgacttgccc
     4741 cgcgatggcg atgctggagc ccgtgacgtc ggcgttgacg accgcgccgt tggcaaccga
     4801 cacctgctcg gtcgtggtga gcttgccgcg tacactgccg tggacgacga cttcttcgtc
     4861 gctcgagagc tcgccctcga tgctgagacc ttctgcgatc accgtgcctg ccatgctgcc
     4921 tctactcgtg tgatgtcagc gcctacgcga ggtgccgaga tccaattcca cttcggtatt
     4981 caaacgcaac gcgacccgag cgcccggctc gatcgcaatc tcggcggctt ttagctcgcc
     5041 acgcacttgc gcgcccgcgc gaatcgcgat ctgcgctcgc gcggtcacgt caccgagcag
     5101 cgtacccccg acgtcgacgg cgtcggcttg cacgttgcct tccacgcggc cgccggccgc
     5161 aatttcggcg tcacctgcga cgtcgatgtc gccgcgcacg gcgccgtcga cgcgcacatg
     5221 cccgtctccg ctcacccttc cagtaatctg ggtggatgag ccgatcacgc tttgctctgt
     5281 agaatgcctg gccatacgga acgcgggcgg gacgctacac tgacccacgg tcgctcggca
     5341 acaggcgcgg caaacttcgc cggcaaagca gcctggccac agctttccgc gggcccgaag
     5401 gtcgggtccc ggtacagaac tattctggta aagcggcgta atagctgcgc gtgctgggtt
     5461 ccgtccgctg gtttagggca ccgcccgtga agcagacgcg agccaagagc cgctggcgat
     5521 cagcttcttc gagaggggct ggtggctgag ctcttagcgg gccggatggg ctcactttgc
     5581 cgcgaacgtt gccgccggtc tcgtctcgtt cggcaacggg cgttcgagca cgggcggggt
     5641 ggtgagccgc aagatttctc gggcgctcgt gaccgaatga tagaggccgc gggcgtaagc
     5701 aatgcgacgc tgttccgggc tcaaatcccc tggcctgctc tgggtggcgg acgcgagcaa
     5761 caccgcggcg gccacactga cgttcaggct ctcggtgaag ccgcgcatgg gaatacggac
     5821 actccgcgaa gccgcgcgca gcagctcggg ccggagcccg ctgtgttcat tacccaacag
     5881 caaggccaac cgcgggatgc gcgagagctc atcgggcaac aactcgccat cgggatcagt
     5941 ggccacgagc tcgaaacctt gagcgtgcaa gcgccccacc gcaaccgtgg gatcggaatg
     6001 cacggtcacg ttcacccagc gctgcgcgcc gcgggtcaca ctgcgcgcga acagcaaacg
     6061 ctcgtcgcgt ggcacgacgt gcagctcggg caagccgaac gcctcgcaag ttcggattac
     6121 ggccgcggca ttgtgcgggt cgtgaggtgc gtccatcagc acggtgactg cgctcacgcg
     6181 ccgctcgatg atctgcagca agcgctgtct gcgtgcctct gacgtgagcg gctccaagcg
     6241 ctcgatcacg ctttccgtcg tccacccctc cggccagggc tgcgcgagtt cactcgcttc
     6301 tccgagattc tcactggttc gtcgcatcgg ttctcagaag ggtggggcgg gccgagccac
     6361 acctgcctga aagcgggggc tttcctgccc cgctgggcgg cattatcttg tggaccttga
     6421 tccaaaacgg cccggcccgg cgcgggcgct gtgctacccc tatcgcacgg agcagtccat
     6481 ggcatccagc aacaacgtgc aagtgttcaa tgatctcaac ttcgaacaag aggtgctgtc
     6541 gtcgggcgtc cccgtcctcg tcgacttcac cgccagctgg tgcggcccct gcaagctgct
     6601 ctcgccgatc gtcgatcagg tcgccgatga gctggtaggt caggtgaagg tcggcaagct
     6661 cgacatcgat gattccccaa tcacggcggg taaatacggc gtgcgtggcg tgcccaccgt
     6721 gatggtgttc aagaatggcg agcgcgcggc ccagcacgtg ggtttgacca cgcgccaaaa
     6781 gctcatcgac ctcgtgaagg tgtgaagcgg cgggttcggc cgagccgcgc tcgtcgttac
     6841 gcctggctcc gccgaccaac ttgcgtcggg gtgcgccgtt tcgtgcaccc cgacctggat
     6901 tttgtcctag gcttcgagac gacgtgaagc cgatttcctc gtcgaaagag cgggcttggg
     6961 ctgttcgaac gcagtcggtg cggtcgaacc ggaccgcccg cggcacggca gcgccactcg
     7021 tggcgctgca agtcgcggcc ccgcaagtcg cggctgcatc gatcccagcc gccgggttga
     7081 tgttggcgct ttcgtgtgct ggctcgggcc tcgagccgcg ctcgtccgat gcgccgttga
     7141 tcgtagaggg cgctgcactc gcgccgtaca cgaaccctgc gcacttccga tatcaccccc
     7201 gagagcgagc acgcactcgc gcggaacgtg agctctcggg agggcaacgc ttgctggttg
     7261 gcgaacgtgg cgagcgttgg ctgttcgacg ctcgcagcca atccatctcg cccggcggca
     7321 tgctggcacc cgagactttg atcgcggttc tgcaggacgc ccaaagtttt tggttcattg
     7381 gtgagagcgg gaccagctat gcggccaatt ctgcgctagc tccgttcgtt cgctctagtg
     7441 ctccggtcga ggcattgaag cgggtgaccg cggcgggcag tacgatcgag ggcgtgaggc
     7501 aggggcgcga gctcttgcgc agcgcggatt tcgggctcag ctggagcaat cttccgctcg
     7561 ggagcgcggc catcgtggac gtcgcgttgg cgccggatgg ccgcggtcta gccctcggaa
     7621 cccccgagag catgtggcaa acgcaagatt tcggtgcgac gttccagctg atcccgaact
     7681 acggctcgaa cggaacgctc gagctcgaga cttcacccga agggcagatc ctcagtcgtg
     7741 gaccgctcgg aacgcggctc tggaacggaa ctgatttcgt aacgatcgcg ccggcaactc
     7801 cgaccaaatc cgcagcgcgc ggctacccgg caccgccccg cggccctgac gcgggcgcga
     7861 ttcgtgaggg tcgcgcgacc atcgacggcc cgcggtattg ggagctgctg aaccttcggg
     7921 gcacgtatcg tctgctctcg ggaccgctgg atggcgtgct gcacgaggtc gaagttccgg
     7981 ccgcgcaggg ctgtcgcgcg gtacggctgg cggcctctgg ccgctacgtc agcttcgcgt
     8041 gttttcgcga cgcggccgag gtcgctgcgc gtgccgtcga ggtttacgcg agccgagact
     8101 cggggcgaaa cttcgagcgg ttgcctgggc agctgcgcgg cagcttggtg agcttccgtt
     8161 tcgcgctcga tgccgaggga cactttttgg ccagtggcgt gtgcgcgccc ggtgcggcgg
     8221 tagccggttg cgcggtgagc ggcgtacaac gtcagaggct cgcctcggat gaggcgcgcc
     8281 aaaaacaggc ggcatccaag cttcctaccg agaagaaaca gagcgagaaa aaacagagct
     8341 cgagtgagaa gaaaccgtcc gaggcggcgg agctcggagc agccaaccaa ctcggggtcg
     8401 tggtcccgac gccggccttg ctcgagtcgg cgttggcgct ggcggtttcg agcgacggca
     8461 agttcgctta cgccgtcgct cgacgcaaca aagcctccgg tttcgcgctg ttcgtctcgc
     8521 gcgacggcgg ccataccttc acgccagagc cgctcgattt gggccaaatc gcttccgacg
     8581 acgatgacag cctggagcgc gctgccaaca ctcgtgtaga gagcctctcg gtcgcggaag
     8641 atggatcggt cgccatcacc ttcgtgcact acggccatcg ggtgctggcc gtcaccgatg
     8701 agcagggcaa ggtgttggcc gccgccgaac cacccgaggc agatgcttgg ctggccgccg
     8761 cggggctgcg cgcgctggcg attgcgccta aatcgcgcca agtctgggag tcgctcgatg
     8821 gcggcgtgac ctggaactcg gcgggggtgc tgcccaatga tccgtgcccg ggcgaggacg
     8881 attgcgaact acccgtcttc tgcgcgtccc atggctgcgt catcggccgt gatctctcac
     8941 gcatcggctg gggactgtcg gagggggacg aagcaggtct gctgcccccc ccgctccggc
     9001 cgctgcgccc ggtttcggat cggcgtctac gcacgccgct cgcctgtaac ttggttcccg
     9061 gcaacttcac cgcactgccc gaaggcagcg cgcctcccga cgcacaacaa gccgcgctcg
     9121 gaggtgtgca gtggttctcg ttcagtgaag acccggcccg tgccgaagtg aagctattca
     9181 gcagtagcgc ggggaaagtc agcgccacga ccttgttcga agcggtgccc acgcccgaga
     9241 ggtacgccta tcgcgtgctc gaccagatcg agggcgtggc cgccattcgc taccgagttc
     9301 cggaagaagg ctcgggcaag acccgcttga ccgacgtcga ggccgcctgg agcaacctca
     9361 tcgaacacaa gacgctgcgg cagaagctcg gcgatggcgg cgagtacttc gcttcggact
     9421 acgtgcgcgg caaatccgcg agctttcgcg ccgatcccga tctcgtgagc atcgccgccg
     9481 ggggcctcta tttgcgcctt cacgcggcct cacagacgga tcaaccgacc ctgttcctgg
     9541 acggcaagca cgtcgaagta cttgctccgt tgagctggcc caagctcggg gaatatccgt
     9601 cgcgggacga tatcgtgcac gtggctggca agcatgttcc gattgcaatc gtgagccatg
     9661 gtgcagcctt ggtccgcgcc cgattgaacg agccctccgg ccccagcttc gacgccgccg
     9721 cgacgggaat gatcgatccg gcgggcttcg gcctcgtgca gctcagcagc ctcacctatg
     9781 tcgatggtcg ccccgcgttg cacgtcgagg aacaagatgg cacgggggcg atcgccaacg
     9841 cgagagtgtt cccgattcgc gcgcaggggc ctgccgtcga tccgccactc ctcgcgccga
     9901 ctcagcgagc gctcgccgaa cgccccgccg cctgcaccgc gggcgagcgg gagcacagcc
     9961 cgcgggtcgt ggctgcgttc caggcgggca cgcgccaccc cgtgattgtg gccgatggca
    10021 gcgaaagcgt gcacaccctg ttgagtggct ttgcagtgct ccatgggagc gtcgagaagc
    10081 cgtgcctagc ggccctggaa gccaaaccgt tgagcggaga caacggcccc gccgctcgcg
    10141 agagcgcgct gatcttccca gacgatttgg agcacgccgt cctattcagg caaatcaacg
    10201 atgcgcgggg cagtcacatc gagtaccgga acatgagctg ccgattcgat gccagcgtcg
    10261 agatcccagc ggagctgcga tctcaggcca acgccgaggc gcgctgaagc ggggttcgag
    10321 cgggccggca tctcacgccg cacccgcatt tcgatccaat ggcgccgcac gatgaggccc
    10381 aagatctgcg ctacgtgata ggggcgaagg ccgctctgct cgtccgcgta caccggcaag
    10441 accggcacct gttggacgcg gagctggcgc gacgcgagca tgcccaacag gtcgttcggg
    10501 tagccgaaac gaggccagag ctgctcgagg ggcagcgtgc gcgcggctcg ggcggacaac
    10561 gccgtgtatc cacattgaca gtccgtcacg cgcaaccccg tgctgaggcg agtggcaaag
    10621 gacagcacct cgcccccgag gcgccgcaac catggcatcc gataagcctc cgggtgggcg
    10681 aagcggttgc ccttcacgta gtccgcgagc ccagcggcga tcggcgcaac gagtgcgggc
    10741 aagtcgccgg ggtgcatctg atcgtcgccc gccatcacga ccaacacatc gtggtcatct
    10801 tgggcggcgc gataacccgt gacgatggcc gcgcccaccc cgcgattctc gcggtgtcgt
    10861 agcactcgga cccgactgtc cccaagtgag ctcgcgacct gggccgtagc atccgggcta
    10921 gcatcatcga tcacccaaac ggtatcgatc tcagccggta agcggcgcaa catgcgcccg
    10981 agcaggcgct cctccttgta cgccgggacc acgacagcca ggcgaaggcc gtacaacatg
    11041 gctcgggcgg gtagcacgcg ccgcgtttca tggcgaggtg tttgcgctgg tgcgtcgcga
    11101 tccgcacggc tagagtccga gctccgcatt tgaactctcc cgcaccgtcg cccacgagct
    11161 cgaaagcccc agcgtcgagc cctcccgcac gcccgggttc gggcaccgga tcctcgcgga
    11221 cgacggccgc gctttcaccc cctgcccctc cccgtacctc tgccttcgct cgcgttcgat
    11281 ggctgctgtt gggttgcgct ctgctcgtac tcggcgggtt cgcgtggtct tggctcactt
    11341 acccgagtga tcgcacgccc gagggcgctt acctgcgcgt ggtcatcgcc gtcaatcgcg
    11401 accgtcccca ggattttttc gcctatctcg agaccccagc ccagcacgcc tgcttcacga
    11461 tcgcggatta tcgcaggcgt gcgcgtgagc tcgtcttgaa gcactatcca gaaccgcagc
    11521 gcactcagct cgcggcacaa tatcgggtcg aggccgacgc caaagacggc gcggacgtgc
    11581 tcgcggcgta tgcgcggagc cgcggtttca tggcgcggct ccgccgagac ctctccggca
    11641 tcgccaaaat cgagcggaat ggcgaacggg ctacggtgga gacggcgcgc ggcacgcgtt
    11701 acccctttcg catccgcccc gaaaacggaa tttggggcct gacgctgttc acggccgagc
    11761 tcatccagga ggctgaaaaa gccgctcggg attatcgaat catcgagaaa gccgcgcagg
    11821 actacgagcg cgccaagacc ggcggttgac gcctaggccg cacacgcagc ggccgcaaac
    11881 acgcggcagc ggccgctccg cagcgttgtg ggacggcgat ccgcaggctc agccgcccgc
    11941 tttggccttc aaatcttcgt aacgctgccc ctcgggttgc cccgtctcga ccggcagcga
    12001 gcggtggtgc caacctggca aaggtcggcc gaaggcgtct cgcgagccgt cccagcccgc
    12061 gctcatgtcg ctaagtgaca cataccccgc ttgttgcagc agctctgccg cgcggcgcga
    12121 gcgaccaccg gccttgcaac ccagaaccag gttggcatcc gtggcaaaga gggcttgcat
    12181 gaccttcaag aactcggggt tgggagccat cccgccggcg cgttggtgag cgatcgggat
    12241 gttgagggag cccggtggat ggccctgttc gaattcctgt tcgctgcgca cgtcgacgta
    12301 cgtggccccc tgctgcatga gctcgtgggc ctgctcgggt gtgaccgact ggataggcat
    12361 ggctatgcag ccctaacact ggccccggcc actgacaaac cgcagccgac tggcctatcc
    12421 ggacatgact cggaccctcg gctacgcccg atctttttga gcagcccccg ctgctcgccc
    12481 cgtgaaaacg ccccacggag tgttagggtt ctggagcaaa taccctggaa gtgatggctt
    12541 gatttcggaa cacaaagcgg gtcgggtacg gctcgttccg ctcggcggac tcggcgaaat
    12601 tggaatgaac tgcttggccg tcgagcaaca cgacggcatc ttggtggtcg actgcggcgc
    12661 cggctttccg gaggacgacc gcggagtcga cgtgatccac ccggactttg cgtggctcgt
    12721 cgcgcaaggc gagcgcgtca agggtgtgtt cctgacgcac gggcatgaag atcatatcgg
    12781 cggcttgccg tatctgcttg ccgagctcga cgtgcccgtc tgggggccac cgcacgccct
    12841 cggcttggtg cgccgtcgcc tggcggagca cgatttcggg cctcacgaag tagatctgcg
    12901 cgaagcccac gccggcaacg tatacgaggt cggccccttc cgcgtggagc cgattcgcgt
    12961 cgcgcattcg atcgtggagg ccagcgcgct gtgcatcgag accgccatgg gacggttact
    13021 gcacaccggc gacttcaact tcgacccgga tccccccgac ggcgaaccca cggacgagct
    13081 tcgcttggag gcactcgggg acgcgggggt gcagctgatg ctgagcgaca gcacgaacat
    13141 cgacgtgccg gagcgggcgg gctcggagcg cagcgtggcc gagtgcgtcg aacggctggt
    13201 ttccgccgct gaacagcgcg tcgtggtcgc gatgttcgca agcaacatcc agcgactgat
    13261 cacgttcggt gagatcgcgg tccgaaccgg gcgcaagctc tgtttgctcg gcaggagcct
    13321 cgaaactcaa tacgccatcg cgactcagat cggtcgcgta cactggcgta gcgacttgct
    13381 cgtggccgca gatcgcgctc aaagtctgcc gcgtgagcgg ctgatggtgt tggcgggcgg
    13441 cactcaggcg gagcgtaact cggcgctgaa gcggctgtca gtcggcgttc acccgcagct
    13501 caagctggag gcgggcgaca gcgtgctgct ctcgagccgc gtcattcccg gcaatgaacg
    13561 agccgtgatc ggcatggtca acgacctgct gcgtcagggg ttgcacgtgc atacgcgcat
    13621 caccgagccc gggattcaca cgagcggtca cgctgggcgc agcgagcaat cgcggatgct
    13681 ggagctggtg cggccgcgat gtttcgtgcc cgtgcacggc acgctgcatc acttgttgcg
    13741 acacgcggag ctggcccgct cgctggggat caccgaaact ctcgttgtgg agaacggcac
    13801 gacgttcgtg tgcgacgggg agcgggtgga gcgggacatc gaggtcgccg ccggtcgcat
    13861 cccgattgcc tatggtggag agccgttgga agcggggatt ttgcaagctc gcacggagct
    13921 cgcgcgcacg ggcattgtcc tgatttcgtt ggcgctcgac accaacaacc gtctggtcgc
    13981 tccccccgcc gtcacctcgc gcggcgtgcc gtgcgtggac gaaagcccgg atgcgctacg
    14041 cgcgcttgcc gcggaagcag cgcgcagcgt gaccaactac cgagacggca gagggctcgg
    14101 cttgagcgag ttcgtacgcc gcaccgtgcg tcgcaagctg gaagatttgt ccggaactcg
    14161 accgaacatc gaagtcacgc tgctcgaaac ccactgagct ggaagccacg aagccgcggc
    14221 agccgtgaac ggcgccgtgg gatatcggtt gattgcgttc gattgctttg gggggcgcgc
    14281 gcggtgttgc gggggctcgg acgcgaacca actgagcggg cgccgcaatt gtaccgagcg
    14341 gcgcgctctt tattgtgccg ggcccctaac tcgggggctc tctgcccgcc tctttccaac
    14401 gatctggttc cgcgcgtcgt ctccgtacgc ggtcactcct gctcgatggc agttcattac
    14461 aactcggccc aatgtggcga ggctcgtgtt atggatgggg ctgggcgttg gtgatgcgtt
    14521 ccagctgaag caggctggag caactctatt gcttgggtct gattgccagt tcgtaactgg
    14581 cgggttgtaa tcggcgggtt ttggaattgg cacaactgag gagtccagac ggcaagcggg
    14641 tggtcctgct ccagtcggag cacttgatcg gccgaggtga acagtgcgag ctacggctga
    14701 acgcgagcta tgtatcggct cagcacgcgg tgattcgatg gagtggcgcg cgctgggagg
    14761 tcttggatcg aggaagccgc aacggaacgc gtgtgaacga agaatcgctg caggcatcgc
    14821 gcgtgtaccc gctcgcgctc gggtccaaac ttcgcttcgg ccaccgcgac gagtgttggg
    14881 tgttcgagaa cgatgaccca cccgagccca cgctgtttct gctgggcacg gggcaggcgg
    14941 tgtcgggcaa ttcaggtgtg attgggattc cgtccaacgc cagcgcagaa ggcacactga
    15001 tgcgggcccc ggatgggagc tggaagctcg agtttctgga cggagtgacg cgcccgctcg
    15061 ccaacggtga ggagttcgaa taccgggggg tgaagtatcg attttgctat ccgtactgtg
    15121 ccgttgccac cgcgagctcg gcggaatccg cggcagaagg gattctgtgc ttcaatgttt
    15181 cggcgaacga ggagttcgtg gaaacttggg tcgagtacgt gtggggtcgg gtggcgctcg
    15241 gctctcgcgc ccacaattac ttgttgctca cgctcgctcg ggcgcgccag gccgatcaga
    15301 gaaacggtgt gccggagtcc gaatgcgggt ggctcgataa ggaggagctc gcggcgggcc
    15361 tgtgcatgac cccgcagcag gtggacgggg acgtcttccg agcgcggcgg cacttggggc
    15421 acttgacgtt gcgtgaagcc gcgaccgtgg ttgagcgtcg gccgcgcacg cgtcagctgc
    15481 ggctcggatt ttcacgcacg cgcatcggag tcacggcgca agaagggcct acggccgcta
    15541 caccgaagcc gccaatctcg agcgacgtga cgcgaagaga actctgaaat tcgcgctcag
    15601 acttcgcggg cgcgagagcc gagttcggcg ttgcaagtga ggcacttctt cggaacgtga
    15661 tccttgagat aagcctctcc gcaggtgggg caaaggcgtg cttcgcgttc gaaggagatg
    15721 ggcttgccgg tggctttgca gtgacgcccc gcaacttgaa cgtcctcgat gtttacgagc
    15781 tcgccgtcgc tgtctttggt ttgaggcaag cgctcgatct cggctcgaga gagcgcaacg
    15841 acgtcaggca cccgttgccc ggcttcactg acgatccacg ccgctgcgcg ttgatgcgcg
    15901 tcgagcggga accggagctg ggcgttcttg ccgcgaatta gaaggtcgga tttgtcgagc
    15961 gcgatgcgct cgatgtcaca ccacaagagt cggttcagct cgttgtcttt ctcgagcgcc
    16021 acgccggctt cccccacccg aacgttggcg agggggccac ccgatttcca gagtgcccac
    16081 gccaaaaccg ccgcgccgga cgcgacgagg acgggagcat aggagagggc ttcgtcacgt
    16141 atccattggg cgtagacgcc cgctcccagc accagcgcgc cgcccattcc ggcaaaggcg
    16201 gtaacgcgcg atgcgtaggt gggttcagcc gtgaagcggc gctctcgacg ttcgcgtttc
    16261 ggggcagact gcttcttttt ggccttcttg gttgcggtca tcgaggggtc cttgagggtg
    16321 cggaaggcta caccgaaata ggcagccgcc ccagccggaa agcgtgtcga tagcgagcca
    16381 cgacgctctg acggcggtta gaatcgggcc atgggcggag ccaagcgctg gccaattttg
    16441 attttgccgt ccctcactct cggcggggcc gcgacgctgg tcggctgggg gagcctgaac
    16501 cgtgaggagt gttcggcgcg cgtgtggggt ggcccgacgg atggtttggc ggtgttcagt
    16561 gggtgggtgg agtcgtgcgt gaatggcccg acgctcgccg gaggacagct gaagctcgag
    16621 gcacgagcgc ggccgggcgc gcaattggct ggtaccgagg tcacgttgga cgacgaaggc
    16681 cgggccgagt ttcatctcga cttcggtgac cagccgccgg gcgagttcta cttgcgcgtc
    16741 gagcacgcag gacgactgct tgcggaaggg cgggtggcgc tgagccgcgc tcgattcttt
    16801 ggagcggcag gcgaacgcgg tggctacttg actagccgaa gcgcgggagc gctggaggtg
    16861 cggggcgcgc cggagcgcgg cgcattggct gtgccgttcg acgccccact gcggctggag
    16921 gtgcgggcag aaggagctcc gctggcggga gtcgatctcg agctgcgcgc ggaaggagcg
    16981 cggctctcga cgaaccacct caagaccaac gggcgtggtg aagcgcaggt gatagtgacg
    17041 ccacaggagc acaccgtgtt cttcacggcg cgggccacca cgattgacgc gggcacggga
    17101 gagtcacgga tgggagagct gcgcagtagc gtccccgtgg tgccaggcgc ctgcgtggtg
    17161 gagcgcggcg aggaacaccg gttcctggtg aagtctccgg tggagcggga cgcggtgttc
    17221 gttacgttga tcgatgacaa ggggcgcctg gccggcagcg ccgtccggtt gagaccaaac
    17281 ggggtggggc aagcgctggg cacatgggat gtgagcgagc cctgggcttc gcaggcgaaa
    17341 tgggccgtct gttcgagcga tccgcgggga agcggcgcgg cactggtagg ttggcccttg
    17401 gcgatcggcg tggatgggcc agcgcatacg cgcgatgtcg cagacgtgtt gctgttggac
    17461 ggggaacccg cacgcaacgc acgcaatgcg ctgcggcgac gctgggtcca tcggctggcg
    17521 ctgatgtggg gagcgctagg cgcgggcctg agtgtggcct gggtttgggt tagttcgaat
    17581 cgggagcggc gcagaatcga gcgagagctc accggagtgc tggacgcgcg aggggtcgag
    17641 caactgactc agactcaggc cgggctcagg ctttgggttt tgtgtttgat tgcgctcgga
    17701 ttcttggggc tggtggcgtt actggcgctg cggcttcgct gagtcgctag ttgagtggag
    17761 cggtgattcg ttcgagggtt tagatcggac acgggccgcg aacgtcgaag gtgcaccatg
    17821 tctgagctga aactcacgcg tcgtgtcgtg ttgtcggtaa tgggggtcga aggggggctg
    17881 ttggccgcaa gcgcagtcgt gggtacgagc gcagtggtgg ccacgagcgc ggaagctcgg
    17941 ccgccgacgc cggcatttcg actcgatctg gagacggaaa caggccaaaa gctccgccgt
    18001 tactcgcacg ctggcagttg gttcgtgctg ggcacgccgg gcgagcgata cgcgattcgc
    18061 gtgacgaatc caacggcgga gcgggtcgaa gcggtggtga gcgtagacgg tcgagatgcg
    18121 atcagtgggc ggcgtgggaa ctatttcacg gagcggggtt acatcgtgcc tgcctatggt
    18181 tcggtgctgc tcgaggggtt tcgcagcagt ctcgaagagg tgagaacgtt ccggttcgca
    18241 ccagcatcgg agagttactc ggcgaggatg ggcaccccgg agaatgttgg gatcattgga
    18301 gtggccatct tcaaggagcg tcgacgggtg cgacgcgcgc ccgctccgat cgcgaaagcg
    18361 gatgaaggct taacggagcg ccgcgaggcc cccgcgggaa agcgcgcggc aggctcgagt
    18421 tcgcccgcgc ctgcccctgc tcctgcgagc cgaagcaagg gtcgaagaga gagtgctggg
    18481 gcgagcaata tcggaacgga gtacggtgag gcccgcgaga gtcaggtggt ggaagtgccg
    18541 ttcgtgcgac gggcggaacg gccggacgtc gtgctgacgg ttcgctatga cgacgcagac
    18601 ggcttaacgg cgcgcggcat cgagctgtac ccggtacctc gagaacccgt cgcgcgtccg
    18661 cccgcgcctc cgcaagcgtt cccgggtagt cgcttcgcac cgcctccgcc ttgaatcatt
    18721 tccaaggttt gcgccttgcc gcgaagagac tcaccggacc ctcttggcct gcctcgcgag
    18781 taacccggga tagttcacga ccgagcgggg tgtgtctggg agggctctcc cgggagagtt
    18841 tccggcggga gagtttcggg ccggggcggg agaaatgtga accgcggtgc ggagctggtg
    18901 ccactggaag cgggaagacc ttcgaccttg ccgactccga gccccgaggc gttgagcccc
    18961 ggcctcgagt cgcccgcacc cgctctccgc tggtgcgttg cccacgagga agccccgcta
    19021 tgaagtgttg gtttcacccc atcggtgtat ccacgatcgc gctctgcacg gtgagttgca
    19081 gccaagaagc agagaagccc acgacgtcga gtggcgtcgc cgcgagcggc aatgtcagcg
    19141 ccgcgggaaa tccaggacta ggcgccaatg cgggagccgg tggtggcccg gcgatcgcga
    19201 ccggcggcgc accactcacg attggcagca gcggcggagc cactctcgtg ccctcgattc
    19261 aaggcggcaa caacaccggc ggtggcacgt cggcacccga gacctgtgcc gccacttacg
    19321 ccgataccgg cttggagccc gtgtatcttg ggttcgcgtt cgacgtctcc gggagtatgg
    19381 gcaaaggaga cgagccgtat cacgacgtcg aattgaaatg gaagcctgtg gtgtcggcga
    19441 ccaaagcctt cttcgcggat ccgagctcgt ccaagatcag tgcgtcgctc agcttctttc
    19501 cggctaagaa cgacaagtgt gatgcgtcga gctacgcaga gcccgacgtc ccaatgacgg
    19561 cgttgccctc ggacgcgttc gcgagtgcca tcgatgcgat tacgccgaag tccgccgacg
    19621 agtggcgcgg gggcactccg acgctggccg tcatcagcgg cgtgagcggc tttttgcgga
    19681 gcttccgggc aaacctcggc gccggcaagt tcgcgctggt gctcgtctcg gacggctatc
    19741 ctcaaggttg cgaccacgaa gaagacgaga tcgagaccgt tgccgaggaa gtcgcgcgtg
    19801 tcgcaaccga gttcccgact tacgtgatcg gcgtctccaa ccccgccggc ggtccagaca
    19861 ccgtcagcaa cctgaaccga gtcgctgaag ccggcggcac cgagaaggcc ttcctaatca
    19921 aaaccgggga tccagagcag acgtcgcggg acttcaagat ggtcatcaat gcaatccgtg
    19981 gccgcacatt gtcctgccag gccaccattc ccaagccgcc ggacggcgca gttctcgaca
    20041 ccagtaaggt gaatgtcagt tacacgaaag caggagagct ccgccctctg gcgtacgatc
    20101 ctgcgtgcac ggaggcgggc gcctggcgtt tcgatgatcc aactcagccg acgcgcatcg
    20161 aactctgtga cgcggcctgt ggcgacgtgc aggccagcgc ggacgtcaag ttgagtgtgg
    20221 agtttggctg cgagacgcgc cccgtcatcc cgcgttgacg agccgctcga tccgggttgg
    20281 ccggcgacga agagtcgcag accaacctgg ctctccgcgc tatcgagggg cagagaacag
    20341 ccgctcgaaa acgcgaatca tgggctcgac gtccgccgtc gccgccatct ctcgacacga
    20401 atgcatactc agcatggggt ttccaacgtc gacgccgcgc acaccgaggc gcgatgacaa
    20461 gatggggccg atcgtcgaac cacagggcat gtcgttgcgg gccacgaagt attgaggctc
    20521 cacctcggcc gcgcggcatg cacgtacgaa tgccgccgcc ccgtcggccc cggtcgcgta
    20581 ggactggttg ctgttcactt tgatcacggg cccggcgccg agcactggtc ggtgttgctt
    20641 gtcgtgtttg tcggcgtagt tggggtgaac cgcgtgcgcc atgtcggccg agaccaacag
    20701 ggaacgagcg agcgcacgcg gcaaagcgtc gccgcgccac tctccaaacg caccgaccaa
    20761 ccgcaagagc acgctgtcga tgaattgcga gcgcgcgcca acggagcttt ggctgcccac
    20821 ctcttcgtga tcgtagagcg caattagccg cgtgaaatct accccttgcc cactccggac
    20881 caacgccgag caggccgcat ggcaggaagc caggttgtcc aagcgggcag agaacaagaa
    20941 ctcgtccgct ccgccgccca gactgcccgc ctgtaaatcg tagaggcaaa gctcgtaacc
    21001 tccgatatct cgagcggtga cgccgggcgc tgcgtccgtc gcgaggctgg aggccacgaa
    21061 ttcgttcaag ctctgcgccg tcttcgattc gagacccgtc acgggaatga ggtgttgctg
    21121 ggcgttcaat tgaagccctt ccttgttgac gtcgcgattg aggtggatcg ccaggttggg
    21181 aatcctgaac aagggtcgct ccacgttcag cagatgctgc acgccgtcga gcgtgaccac
    21241 gcgcccggcg agccccagat cgcgatccat ccaagtcgac agcaagaccc cgccgtagac
    21301 ctcgacgcca agttcacggt accccaaatt ctctacgtcc ggacgcgggc ggactcgcag
    21361 attcggggag tcggtgtgag ctcccagaat cacgaagccg ctctccgcgg gcgccttgtc
    21421 ccccgcgatg aacccaatga tgctgcccgc ccgcgtgaca tacccccgcg tgcccggctg
    21481 aagctcccag ccatcctgct cctggaactt gcgaaacccg acctgggcga gccggcgctc
    21541 gacctcggcc accgcgtggc aaggtgtcgg cgaagcctgg atgaatcgaa gcagatcgcg
    21601 ggcagcgtcg aggggcgctt ggtgagcttg catgggggca ctatactccg ggcgcgatgc
    21661 tcagcccgga tgtgcgagta gaaggcttca gcgccgaaga atggcaaagg ctcggtcaga
    21721 ttttccgtgg cccgagcccc gagtttccgc acgccgagcc gcgggaacgg acgaacggat
    21781 ctgccacgga tcggtcggcg gacgtcggcg tcggcacaca atccaggcgt ggctcggggg
    21841 gtttgatcgc gctcacggac gcgggacgct tacgcaagct gctgtcgacg cagcgtggac
    21901 gcttggaccc tcgaggctac gccgggccgc tcgatctggc cgatttgtgc gcacgtcacg
    21961 gagcgagctg ggccgtcgag gtgacccacg gaacgctgga acggctagtg gatcgcttcg
    22021 cggaacggct gcgtccggcc gacacttacc tttcacagct gctcgagttc ttactggtgt
    22081 tgcgcgagct cgagagcgag ggggatttgc gggtgtttcc gtggccggta gcggactggc
    22141 ctatcccgac agagcgggcc gtcgtgcgcg cgatggaagc gctctgtccg gtgggacggg
    22201 tcgcgctact cggggtgttc gcgcaaggcg agctgtatac ggcagccgcg ttccgtcgcg
    22261 gcgcgagcgg catcgatgcg atcgtggggc cttcggagct ccgggctcgg atgggtttgt
    22321 tgtccgggga ttggcagcgt gattaccgtt ttttggcgtc ggcttgtgaa acgctgctgg
    22381 ggccactggc gctgggttgt ttcggcgagc tgttcacgtt ccagacgctg gcgaaagatc
    22441 cggcccccgg agcctggact tcggcggtgg catcgcgcga gatcgtgctg tcaccggtta
    22501 gtcccgcgtt ggcgctgcca ctcgggctcg atgccggcag agcactctgg gcgcaagtcc
    22561 gcgggttggc ggagcgcttc gggggcagtg gctggctcgg gcaggccagc aagagagctc
    22621 cgttcgaccg attgcccgcc ttcgaatccg acgtggtggc ttggctcggc ttcgaccccc
    22681 ttcgtctact atcccgttgg atgtcggcgc gagattgaac atgaggaacg ttgcggtcgc
    22741 gggccgcatt caacaccgct tggcgctcgc gatcgtgctc acggcgctga tcccggtgct
    22801 ggtcgcgatg tggatggccc aaaatacagt gcgccaaacc gcggctcgtt tttacattcc
    22861 ggaggtcggg atgcatctcg accgttcgct ggatctgtac aaagagctcg cgcgcagtgt
    22921 caaagccttg atgcgtgaag aggcggccgc catcgcagag cgtcaccgct tgcgcgaagc
    22981 ggtccggcta cgagacagcg cggccatgca gcaagaactc acgcgcgcgt tcggtgaaca
    23041 cccgagtctg gtatcgctca gcatccgcga cgccgacggg aacgagctat cccgggtaca
    23101 acgaccgaag ccgctcgacc cggcccgcga aaaggacctg gaagtggagc gcagtctgcg
    23161 agaagcggac gacggcgaga tgttgatggc ggtgtttgcg acgccccgcg accgcttcga
    23221 cgagctctcc gagatgaacc agttcgtcga tacctaccgc aagctcgagc atcggcgcga
    23281 ggcggacgaa agaagttatg tgctggcgtt cgcggtactc ctgggtatca cgatcgtggc
    23341 cgccatcggg gtgggcgtgc tgttggcgcg cagtgtctcc ggacgcatcg gtgagctcgc
    23401 gatagccacc aagtgggtgg cagaaggcga cctcacgatc cgggtgccgg agcgcggcaa
    23461 agacgagctt tccgatctcg cccgtgcctt caatcgcatg ctcggagaag tcaccgagag
    23521 ccgctctcga atcgagtact tgcagcgtat cggcgcttgg caagaaatgg cgcgtcgcct
    23581 cgcccacgaa atcaagaatc ccctcacacc aatccagctc gcggtgcagg agatccatcg
    23641 gcgctacacc ggctccgatc cggcatttcg ccggttgctg gacacgacgc tcgagatcgt
    23701 cgaggacgag gtgggcacgc tgcggcgcct agtgagcgag ttctccaact tcgcgcgctt
    23761 gccgcaagcc aagttggaac cggcggattt ggcggatttc ttgcgtgaac tcgccactca
    23821 gttgcgcatc gaagatcgag acgcggacct gcccgaagat ctccgtgcct gcttgcaagg
    23881 cacccacatc cacctcgact tcgaggtacc cgaggaggcc gcggaggtac tgatggatcg
    23941 tcagatgctc cggcgcgttt tgatgaattt gatccagaac gcggcccaag ccctgcggga
    24001 tacccgcgaa gatgcggcgc gcatccgcgt gcggctcgcg cgcgacggcg atttttacgt
    24061 ggtggacgtg gaagacaccg gacccggcat cccggaaacg ctgggtgagc ggatcttcga
    24121 tccgtacgtg acgagcaaac cggacggaac cggcctgggc ctcgctatcg tgaagaagat
    24181 caccatggaa cacggcggtc ggatcagtgc atcgacgagc gacttgggag gagctcggtt
    24241 gcgggtgcgg ctgccagccc tggggacccg cgcggcggcc ctagcgagtg ccacgagcct
    24301 cgagcgttcc ggggaattcg ctcgcgcggc cctcatgcga tagttgccga ggggcggcgc
    24361 tggtgattct gcacgcggag gccctggatt tgccgtccgc ggcgcctgat caatagtgac
    24421 tacctaccgt gccttccagt agtcgctctc ctcgatcgca gtccccgttc acgagtgtga
    24481 tttgggtctc gttgatcgca atcgcggccg ggctgttcgt gctgccgcgc ttgcagcccg
    24541 gcaatgcttt gaccgggctt tccggtggta ccgagcccgc tccggacttc acgttgccag
    24601 tcattcacgg tggagagctc gattcgcgac tgcgtctgtc tgcgcttcgc ggcaagttgg
    24661 tgctcttgga tttctgggca agttggtgtc agccgtgtcg cgagcaagcc aaggtattgc
    24721 gcgccgtcgc ggggttgccg gagaatcgag atctcgagat cgtagggatc aacgtcagtg
    24781 accagcccga cgcggcccga cgctacttga aggccgacgc accgccctgg gtggtcgtgg
    24841 aggacccgga gggggtcgcg aactcggcgt attcggtcga gaccctaccc accttggtcg
    24901 cggtaaacgc agacgggaag ctgttcacgg tgcgccgccg cttcgttccg gagcgcgaat
    24961 tgaatgcgat tttagagggg atgcgtagtc aggctcgaag ccagaggcat tgactccgcg
    25021 cggcggctcg gttggatcga gcgggatcca cgctcgcggt gatcgttagg gcgcgagagg
    25081 atcgtagggc ttcggtggct tgagtgcggt cgaggtgcgg gacgtagcgg gcacactcga
    25141 cgacttcgcc ggggcgctgg tcggggcggg cttgccgaaa acgggtgcgt gagcaccggg
    25201 ctttgccttc gccccggccg ctgatgattc cgtgggggac tgcaacagcg gcttggaatg
    25261 cgttcgtggt tcgagctcgc tcggcgcgtt cgaagccgcc gaattctgct cgggcgccgg
    25321 tgcgttgtcg gggagcggct cgaccgcggg ctccgcgcga gtagggttcg gcgctacggg
    25381 cagcccgggg ctggcctgcg gaatggcggc aggacgcgcc tcgagggggg cgttggctct
    25441 agtgcgcccg agccaccagc tcacggctag tccggcactc agcaccgcga cagcggcgat
    25501 cacggcgacg cgaagagccg gcgatttcgc gcgctcgccg ctgcggacca gagtggcggg
    25561 cgcactcggg ctcaaggagt tcggcgtcgg gttcgagcta ctcgagggca gtgcccgagc
    25621 acctgactcc tgacgatagg tcgccgtgcg gctgaggtcg gcgatcggcg cgacctcgga
    25681 ctgacgaact acgagctcca gttcgttgcc catcagacgt acgagggact gggtgagctg
    25741 ctcatcggca atcggaacag agagcgcttg agacagcgcg cgcacttgct cgccagcgga
    25801 ggcgaagcgt cggctcggtt cccgatcaca cgaacgaaag aaccacgcat cgagccgggt
    25861 ccccaactcg ggaaaacgca cagagggtgg cggcatcggt tcgctcaaga tttgaaccat
    25921 gagctcggcc aaggtgttgg cgctccagta ggcgcgcgcg gtgagcaggc gaagggcgat
    25981 gatgccaatt gcccacacgt cggcggcagg tcccgtctcg tggacgcgac cgcgtgcctg
    26041 ttcgggggcc atatagaacg gtgtaccgag cagtgtgccg tctttggtga cgaacaggtt
    26101 ttggggatct ccctggtcgg tcgcgaacct cgagatgccg aagtcgagga tctttacgat
    26161 gaccgcaccg tcttctttgc ggtgcagata caggttctct ggttttaggt cgcgatggac
    26221 gatcccgagg gagtgggctc gatcgagcgc gcgggcaact tgggcgagga ccgagactac
    26281 ggccgagggg gagagcgcgc cgcggcgttc gacgagtcgc tcgaggtcga gaccatcgag
    26341 ctgctccatc acgaagtacg gagcattgcc gagttcggga gcgagcccgg cgtcagtcac
    26401 gcgcacgacg tgttcgctcc tgatcttcgc ggcggctcga gcttcgcgtt ggaagcgcgc
    26461 gatcaggctg gcatcgaacg agggtggttg agagaggggt gccgacggag ccccatctcc
    26521 gggccaggtc gagtgcagca gcttgagcgc gagctgttcg ccggtgtgaa cgtgttcgac
    26581 ttcgtagacg acgcc
//
LOCUS       DASTKU010000822         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1949908, whole genome shotgun sequence.
ACCESSION   DASTKU010000822 DASTKU010000000
VERSION     DASTKU010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949908"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VFQ61_28750"
     CDS             <1..703
                     /locus_tag="VFQ61_28750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958531.1"
                     /translation="AQESFAVPLPGHTLHLEQGQLVNVERATAFLQAAPEAEWPNRRY
                     EPSVRVLKSLTPLTGKKQLPDEQIPELEAGLNELGRMLYGGPEFRALYSLPDVPSLGR
                     KPTFALVLLADESGGAYVYEYEPQSSSFVCAGSTNPVAEYIAGCEMWATDLLALFQGE
                     FGPSCLFFGNSRIWSAAPDHFRLSLLDWMAHFHPLRMPEPCLARYRRELRAANPHHHA
                     IIQSNSRTLSRVASR"
     gene            718..2091
                     /locus_tag="VFQ61_28755"
     CDS             718..2091
                     /locus_tag="VFQ61_28755"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9958532.1"
                     /translation="MKVTICGHAGLFIEAGEERILIDPILRNTPLASGGVVHTLTRML
                     RLADMPAPTLLLVTHAHLDHFDPESLATLPRTLPVIVPGDPPMIEKLRSLGFSKLIVL
                     APWQVFQGRGVTLTATPSGTDIDEFGLVVQTADACFWHMSDAEAELDDAKRVAAQFGT
                     IDLLSAKYQPASRVQSQIFRSLGACSDKHDIQDWLETACACEPQVVFPYASGVRFHGD
                     HAWLNKHAFPFRADEITRLLRLRLGGADRAVTVAPGDVFEIENHRVSHRPQACAFITH
                     DATSGGEVDWEPIEPGTLAGLATDEQRQDLEARLLALFRGAFGRWMAAQSGAEGVLSQ
                     YVEFGVIWQLSVHVSESERLEYFIDFTAPRFAIVEGRHPYANFFAHVAGKGLLRRLNG
                     EAQPEALYSCGDLRLFEKIIGVRDGRVYVPPVQGWELFECLPEPLTYFLRYPRKWGAD
                     LEAAGAA"
BASE COUNT          370 a          707 c          724 g          448 t
ORIGIN      
        1 cgctcaggaa tcgttcgccg tgccactgcc gggccatact cttcacctcg agcaggggca
       61 gctcgtgaac gtggaacgcg ccactgcgtt tctgcaagcg gcaccggagg cagagtggcc
      121 gaatcgccgc tacgagccga gtgtccgcgt gttgaagagt ctgacgccac tcaccggcaa
      181 gaagcagctg ccggatgagc aaattccgga gctcgaagcc ggcttgaacg agctcgggcg
      241 catgctgtac ggcggccccg agttccgcgc gctgtactcg ctgccggatg tgccgagcct
      301 cgggcgcaaa ccaaccttcg cgttggtgct gctggcggac gaatcgggcg gcgcgtacgt
      361 ctacgagtac gagccccagt cttcgagctt cgtttgtgcc ggcagcacga acccagttgc
      421 cgagtacatc gcgggttgcg agatgtgggc gacagacttg ctcgcgctgt tccagggcga
      481 gttcgggccc agctgcctgt ttttcggcaa ctcccggatc tggagcgcgg ctccagatca
      541 cttccggctc tcgctgttgg actggatggc gcatttccat ccgctgcgca tgccggagcc
      601 ctgtttggct cgctatcgac gcgagctacg cgccgccaat ccgcaccatc acgccatcat
      661 tcaatcgaac tcacgcaccc tgtcgcgagt cgcaagccga taaaccgagg aatggatatg
      721 aaagttacga tttgtggaca cgccggcctg ttcatcgagg ctggcgagga gcgcatcctg
      781 atcgatccga tcctgcgcaa tacacccctc gcgagcggtg gcgtggtgca tacgctaact
      841 aggatgttgc ggctggcgga catgcccgct cccaccttgt tactcgtcac gcacgcccat
      901 ctcgatcact tcgatccgga gtccttggct accctgcctc gtactctgcc cgtgatcgtg
      961 cccggggatc cgcccatgat cgagaagctg cgcagtctgg gtttctcgaa gctgatcgtg
     1021 ctcgcgccct ggcaggtgtt tcagggccga ggggtcactt tgaccgcgac cccctcgggc
     1081 acggacatcg acgagttcgg gttggtggtg caaaccgccg acgcatgctt ttggcacatg
     1141 tccgacgccg aggccgagct cgacgatgcc aagcgtgtcg cggcccagtt cggcacgatt
     1201 gacttactgt cggccaagta ccagcccgcc tcccgcgtgc agagccaaat cttccgttcg
     1261 cttggggcct gctcggacaa gcacgatatc caagattggc tggagacggc ctgcgcgtgt
     1321 gagccccaag tggtcttccc gtacgcctcg ggagtgcgtt ttcacgggga ccatgcctgg
     1381 ctgaacaagc acgctttccc gttccgcgcg gacgagatca ctcgcttgct gcgcttgcgc
     1441 ttggggggcg cggatcgcgc ggtcacggta gcgcccggag atgtattcga gatcgagaat
     1501 catcgagttt cgcaccgacc gcaggcctgc gcgttcatca cgcacgacgc cacttccggg
     1561 ggtgaggtgg attgggagcc gattgaaccg ggcactctgg ccggactcgc cacggacgag
     1621 cagcgccagg atctcgaagc tcgcttgtta gctctattcc gcggcgcgtt tgggcgctgg
     1681 atggcggccc agagcggggc ggaaggagtg ctctcccagt acgtcgagtt tggtgtaatt
     1741 tggcagctct ccgtgcatgt ctccgagagc gagcggctgg agtactttat cgactttact
     1801 gcacctcgtt tcgccatcgt ggaggggcgc cacccgtacg cgaacttctt cgcccacgta
     1861 gcgggcaagg gcctgctgcg gcgcctgaac ggcgaagcgc aacccgaggc cctgtattcg
     1921 tgcggtgatt tgcggctgtt cgagaagatc atcggcgtgc gcgacgggcg cgtctatgtt
     1981 ccgcccgttc agggctggga gctgttcgag tgcttgcccg agccactgac gtactttcta
     2041 cgttatccgc gcaagtgggg ggcggatctg gaggccgccg gtgctgctta ggaagcgcct
     2101 ctccgacacg gtgcagctgg ccgtctctta tgcagacggc acgcgctacg gcagcgtcct
     2161 tacagggcgc tgtgaccagc tcatccgtca gaagctgcgg cacgtggccg agctacgccg
     2221 cgagcgtggc ttggcccacg tggtcgagc
//
LOCUS       DASTKU010000823        17044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1279247, whole genome shotgun sequence.
ACCESSION   DASTKU010000823 DASTKU010000000
VERSION     DASTKU010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 17044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17044
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279247"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..2338
                     /locus_tag="VFQ61_28760"
     CDS             188..2338
                     /locus_tag="VFQ61_28760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958533.1"
                     /translation="MLESTARLVSACAIASCWAFFALACAILTPSKSFSQRSLIGCVL
                     AAWVATAIFLVLGYLRWVSLPSLLFSAGGAALAVGRVMQRRVPDWARQVGAWARLLRE
                     LPRRIGPLATAMLAVLGVSYAVLGAAGAVSPLLGWDSLHYHLVHVAQWAQEGGFVPQP
                     GVDAWEYYEHFPIGGEILWAWLCIPTRDTTLLGLAGIAVTGLAGLATYVAARSLSSSV
                     PGQVEVAPGRAVLAGCVVLSPPAVAMFATNGYVDNATFSFAAAGFALLLAAMRSPAPN
                     VVPWIALSFAAFGLAIGVKTTAIGFAALGAVCALVLLLARRAGARRIVGSALLAIPGL
                     LIAVPRLVETWIRRGSPMYPFPLKLGKWVISAGESELVALLTARYADKRLEAAKSELF
                     QLLFVRSVRPDWPHVNFGWGGAALLLAGAIGLGFWLTRRPKDLRVWLFLALICTALPY
                     FSGPNTLGYRIYWWWIIARFMLGALLALVLLASVVPGRWVDYVFAGCSVGGAAYALGS
                     GMCSYRWIGAASVLFSATCIGGLSWIIRRSGYPKLATGLILSGALGLASLLLMLRKEL
                     AYIVLAGALRGQCYEGHPLTHAVLFARPVWDALADAPPRRIALVSGFEGVGHNVFRYP
                     LLGRRWQHRVVYVAPSADGKLRGHPDIYGLRDRPALSFEAWLSRLKQQRVTHVVLVPP
                     FNVEEYRWVFEHKRHFVLEQRGEKDYAFLFRVVP"
     gene            complement(2345..4477)
                     /locus_tag="VFQ61_28765"
     CDS             complement(2345..4477)
                     /locus_tag="VFQ61_28765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958534.1"
                     /translation="MVFIHWRAAPLCGRRRTALSCLGGAVTAAIDEGSLLEAVGQSEV
                     VSGSREAQPWSIRGYIVGACVFVLTCAVYVSALGGDFIWDDHSLLTENSRLRELKSLW
                     FYLTSSFWGTSEQDVDGRIYYRPLSLLSLAIDYRLHGDNPTGYHVTNLLFHASNASLL
                     FGLLRKRGLDAIRSAILSVMWAWFPRLTEAAAWISGRTDVLATTFVLIGLYLTPARSW
                     LRRCAAALSLLIGLFAKEVAMAGVLGCLVLEWHEATGLPVWRRLQRLLPFFGILLVYT
                     ALRTYAVGAALRPNGLPWADRFPSGAEALGRYAWMLVDGWHPSTRVGALDDRTWGFVG
                     LGGLVVLAIVAGVAWLIRRSRAGRPVLDPSGQSALAVLAVSVFLVLHIVPITVNVVAA
                     DRFLYLPVAALVLLLAPLLRDRSLRHPAWILVLLCTLSYAPTTVARSLVWNDEIDFWT
                     TAFKEQRTRNASSRLELGNVYARAGMHSAATAVYLDAETSDDFNYAMTLNNLAVRLLF
                     LGRAKDAEAVQRQVVSRAPHIPKFQFALASIYLAENKFDLALEHVRIAKRLFPKNSAY
                     TKFEETTEDLKKEGERPPFNPDNTRDLLAQASLDSRCFRYKEAIDSLLLASQRPDITL
                     RDLNDMMLFAFNTATPKQLTELYERYRAHGGNSPEFAESYALRMRRVARLRALWPALA
                     PYRTDPERLKHGPVIQIAKPTPSASSSL"
     gene            complement(4557..5135)
                     /locus_tag="VFQ61_28770"
     CDS             complement(4557..5135)
                     /locus_tag="VFQ61_28770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET9958535.1"
                     /translation="MIAKLKRSKRGFTLVELMIVVAIVGILAALAIYGVSKYVKNAKS
                     AEARDALGRMAKDAVGAYQRENMDPNVMALGTSTGISHKLCDSATAVPTGVPSAEKFQ
                     SSPSQWDSGTSTAGWMCLRFSMNDPQYYQYDYQATGVATGGGSFSCIAHGNLDGDTST
                     STFYIAGGVAADGAGNQTAIYAPNIGEINPDE"
     gene            complement(5238..5876)
                     /locus_tag="VFQ61_28775"
     CDS             complement(5238..5876)
                     /locus_tag="VFQ61_28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET9958536.1"
                     /translation="MGTPNVYGPARRARRTDCRARKADRTAPYVFAGQRGFTLVELMI
                     VVVIVSILATLGVYGTRRYISSSKTGEAIQLIGSIKAAQEAYKDETFQYLDVSTAKTL
                     TSNFYPTNPKPGQTKVMWGGGTDDVAKNWATLGVNPGGPVLFVYACSAGGVGDTPTNP
                     GTDITVGSWPTTALGSPWYVVKAKADLSSGGKETVYVAPSFTTQIFSANEGE"
     gene            6218..6703
                     /locus_tag="VFQ61_28780"
     CDS             6218..6703
                     /locus_tag="VFQ61_28780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958537.1"
                     /translation="MRALTPLEAAFGIALAGSVLAVVIPSFVRNLHASYVSEATTGVA
                     ELAARTAARLEAAQTTSSLPESAPLTPSNVPRGVRVRDPEGTWEHPTWRALEFKFSVP
                     HAYSFAFDAESAPDVAKFKVTAHGDLDGDAVLSTIAIDGTFKPGAAPSLSAMDVQHEI
                     E"
     gene            6690..7628
                     /locus_tag="VFQ61_28785"
     CDS             6690..7628
                     /locus_tag="VFQ61_28785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958538.1"
                     /translation="MKSSSARRLGSWLATATLVAGSIGVIAALRAPLAAAHASVQRQE
                     DVFGLPRADLTRVLSLGYRSALADLLFAHVLVDYGLHFQDKRRFEFAASYLETIVALD
                     PKFREPYRLADTLIVLQPTAPRLEDYRAARRLGEQGLREFPYDAELWLIVGQYLAYLA
                     SAHVPSNERDEYRLEGARKLARACELVSSNQNIPYHCITAAALLNQIGQQSAMKSFIE
                     RVMAISDDAEIRSVAGGYLGRVLGQVERDAVEERGRRLRALWSRDLTFVSREMWLLLG
                     PRFDPAMCAGSGHHREPACATSFRDLTEAEQASAGD"
     gene            complement(7746..9938)
                     /locus_tag="VFQ61_28790"
     CDS             complement(7746..9938)
                     /locus_tag="VFQ61_28790"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9958539.1"
                     /translation="MTDKASSDLDIFDGLAAKKSRPAHGMTSVPPPPGSRVPVAPPSM
                     TRQKTLLGLAAPPPGAPTTGRPPPPPPPGSRTGSMAPFPPAPSKLNSPVPPPPPLNIS
                     TPMPLALPPSPSAAAAPAPLLLGPGTGQALVVPPPPPPSLGAIAAPPPPPAPSAIAVP
                     PPPPPPAPGAIAVPPPPPPFTSSNGSSSNGLNALNGLGNGDLAPSEDETTVMGHTSES
                     KLPVEAKLTSESKLPSESKLNGAPKGGGVEVDWDDEDEATTVFDRSQEDNVRSLLRGA
                     PPPPPAAGAPPPVATRPPPPPPGVGSLGRTTISSSRISSAPPAPSLPRAPLPPPRALP
                     SVSAPPVPAALPAPAMPLASSAAEPTISTPLRKVRKPVLIAAAAGGAAVLIGLIALLV
                     PKSGSLVVTVAGPGSRTVSDLEVLVDGQKKCTASPCQVKDLSAGTHLVKVTAPGYAST
                     ADQAVKVASGDEPVLNIVLTRSGGSGLNVRSEARGLKLLVDGKEIGPLPQELPDLTPG
                     EHRVRIEGDRYEPFERTVNVTADRLELIDAKPKVKKGLATFKSGLNADGAQIVLVSGT
                     ERRPVPPLPVSVDIPTDKPYSVVANKRGFQTFEQKLEFEDGQAERTFQIDMVPPVVST
                     SDNANTPTSHGSPGPSRSPSPAPAAIAAVTPPKPAPAAGNGTLNLNSNPVSNVILDGR
                     PLGATPKAGLSVPPGSHTVIFVHPEHGRKAKSVTVQAGKTVGISVRFP"
     gene            complement(10358..13087)
                     /locus_tag="VFQ61_28795"
     CDS             complement(10358..13087)
                     /locus_tag="VFQ61_28795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958540.1"
                     /translation="MGEAAPGDVSSAPVAAVRSEGAAHEWRHLLESARPLPLFFAIAY
                     VVHRGDSLPDRVLSETNRERAVGAALESMGLAAKVEDIHFVSPTQPWVRSPVVRERAI
                     VVARRRGEPRDIYLVDTRRSPDGHLIDVAGLYNLTSTSAADEQGLVVQGDDAAWMIAQ
                     EGAISSVQYATMRGEPVPNGPGWSKRSRLQNALTNYQETGQTSGIGRRSFKLDPPAYK
                     VVLGFSKGALLVDADAHKIRIPLGGGPIEGARYVHDQTPAKARPGNLVTWAVDRVRAL
                     PWIGDTKLQLLKAVAFQGVDQFEQIVGTVTGDDGAESVAEELGDLYAAPSSDGTNAET
                     GWPPPPLVPMLDPPLKGEGKWVALNNDPFVLRNVGAPAPFVYTFIRADKKRRYSQVFI
                     TLWDPRQVELNMVSGTVEPKSATGETGTGMVPRNDRTFGRFVAAFNGGFQAIHGEFGM
                     MADRVLYLPPKPYGATVAKLSDGATGFGTWPEDSRVPEAIVSFRQNMTPLIVDEVPNP
                     YKRHWWGGVPPGWTEESRTVRSALCMTKEGFVGYFYGANLDPDVLALSMKKARCTYGV
                     HLDMNAGHTGLEFYRVGPKGSLPNLGRRLDDVWEAAGPVPGKPGWEFMSRRMIRFMAL
                     MNFPRYINTEGRDFFYLTLRHVLPGDPAPTAIPGEGEGAWRTQGLPQHGWPYAVATTN
                     VRPEASRPFARVGLIKIDPRVVRVAKPGDVAGQRILDFRSVVAEGPAALWHSETTGFV
                     IGNKPPDSRAEKVTSGFPEGSEESARGQAAIGIDAGGMLIYARVTEGPEPGRDAALLG
                     ALLHRMHCDSVLMLPRPLGADFARPDDPQVPASTDRVTLVRGEGNGVRRIFPSTPIVM
                     PKRWAPLQQKSVKLDATDSVPPTVAPESGATEANEPGGPGNQDPGGDADKPGE"
     gene            13262..13597
                     /locus_tag="VFQ61_28800"
     CDS             13262..13597
                     /locus_tag="VFQ61_28800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958541.1"
                     /translation="MSMVARRSFALFGGLFVAACLSPTLPLPPPSEPEVSGPTEQGLI
                     RLTGNAPGGAWMYALNKTSNVGVFNRATPEGRYDLEIPAEVGHRIALWYELDGEQSES
                     LLFTVRTRP"
     gene            complement(13699..15210)
                     /gene="rimO"
                     /locus_tag="VFQ61_28805"
     CDS             complement(13699..15210)
                     /gene="rimO"
                     /locus_tag="VFQ61_28805"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET9958542.1"
                     /translation="MPIDLEATETTEPTAADETAADETAAPEGPTIHFVSLGCPKNRV
                     DTEVMLGVAERSGYAHVDDPKNAEVIVVNTCGFIDAAKKESIDTILELSAYKQEGRCQ
                     RLVVTGCLSQRYPEELSQGLPEIDNLVGSSDMLALERVLGGAAPRVAVGNPADWLVGA
                     QDPRRLSTRGRSAYVKIAEGCNRTCSFCVIPQIRGKQRSRTPDDVIAEVERLVAAGVI
                     EINLISQDTVAYGRDQEGAKGALASLVRRVAEVPGVHWVRLFYLYPEKLDDELIELLG
                     QHPRVVPYVDMPLQHAADDMLRRMRRGHGGQRLYELVDRLRTKIPDLTFRSAFIVGHP
                     GETQQEFDELLKFVEFAQFDRLGVFCYSDEPTARSFELDHKVPEKTARSRARKLSRLQ
                     EQISQNKLKALIGRELEVLVEGPSEEFELVMVGRHAGQAPEIDGGVVLSGGEVRAGEV
                     HRVRITQVADYDLLGEVDDATPPVISAPVVPMRPALSHRSSDGRRVALRTMPS"
     gene            complement(15210..16337)
                     /gene="carA"
                     /locus_tag="VFQ61_28810"
     CDS             complement(15210..16337)
                     /gene="carA"
                     /locus_tag="VFQ61_28810"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012053752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET9958543.1"
                     /translation="MTQKPGHLVLADGTVFPGCVFGADGVATGEVVFTTGMTGYQEVL
                     TDPSYCGQIVTMTAPQIGNTGINTEDAESVTDRPQVAGFVVRDNSAVVSNWRSQETLD
                     AYLARHGIVGISEIDTRKLTRHLRDRGSQNGVIGAGPLEALIERARTVPSMEGLDLVA
                     RVSPRERYAFSESLGAWPMPMQRAGSGERPKVVAMDFGAKRNIFRCLVDIGCDVTAVP
                     ANTSAAEILALSPDGVFLSNGPGDPAAVTYAIDTIKGLVGKKPIFGICLGHQLLSLAL
                     GARTYKLKFGHRGLNQPVKDLTTGRVEITTQNHGFVVDVDSLKGVAQTTHLHLNDGTS
                     EGLEAPDLKAFSVQYHPEAAAGPHDSLYLFERFRNLMLSPR"
     gene            complement(16334..>17044)
                     /locus_tag="VFQ61_28815"
     CDS             complement(16334..>17044)
                     /locus_tag="VFQ61_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9958544.1"
                     /translation="EGAVSARLGLRGWPRAAEDVIVARDVILAEYVKARYHVAHVSTL
                     GAVRILREAKGRGIDVTCEVTPHHLLLTDASVIGYDTACKVNPPLREPEDIAALRRAL
                     ADGTIDAIATDHAPHSPLEKDCEFSAASPGMMGLELCFGLLLGLVGQDGLTLGRLIEA
                     LSTRPARIAGLEAPKLREGALAELCLVDPEAVSTPRQMQLQSKSRNTPFMDRELKGRI
                     LMTLARGKIVFDALGSSR"
BASE COUNT         2866 a         5360 c         5568 g         3250 t
ORIGIN      
        1 gtatttgatc caagcgcgcg cggactacga ctccgacggc gtccactgct atgttctcgc
       61 ggcgcccgag gcgaccatga acaccagcga cgtgttcatc gaacgagaag gggagtagca
      121 agcaacgtcg gtaaaaggcg gggtgccctt ctgttgctag gagaggcgat gtgatacgaa
      181 cgagcccatg ctggagtcga ccgcgcggct cgtgtcagcg tgtgcaatcg cctcgtgttg
      241 ggcatttttt gcgctggctt gtgcgatcct cacgccctcg aagagcttca gtcagcgcag
      301 cttgattgga tgcgtgctcg ccgcttgggt ggcgaccgcc atcttcctcg tcctcggcta
      361 tttgcgttgg gtatcgctac cttcactgct gttcagtgcg ggcggcgcgg cgttggccgt
      421 gggccgagtc atgcaacggc gtgtgcccga ttgggcgcgc caggtcggtg cttgggcccg
      481 tctattacgc gaactaccgc gtcgcattgg cccgctcgcg actgcgatgc tcgccgttct
      541 cggcgtgagc tacgcggtgc tcggagcggc cggcgcggtt tcgccgctgc taggctggga
      601 cagccttcat tatcacttgg tgcacgttgc gcagtgggcg caggagggcg gcttcgtccc
      661 gcagcccggt gtggatgctt gggagtacta cgagcacttc ccgattggcg gcgagatctt
      721 gtgggcttgg ctctgcattc ccacgcggga taccacgttg ctcggtctcg ccgggatcgc
      781 ggttaccggg ctggctggtt tggcgaccta cgtcgcggcg cgttcactgt cgagttccgt
      841 gcccggtcag gtcgaagttg ctcccgggcg cgcggtgctg gcgggctgcg tcgtgctctc
      901 accgcccgcg gtcgcgatgt tcgccacgaa cggctacgtg gacaatgcga cgttctcctt
      961 tgccgcagct ggtttcgcgt tgctgctcgc ggcgatgcgc tcaccggcgc ccaatgtcgt
     1021 gccatggatc gcgctctcgt ttgcggcgtt cgggctggcg attggagtga agaccaccgc
     1081 catcggcttc gcggccctcg gtgcagtctg cgcgttggtg ctgctgctcg cccgtcgcgc
     1141 gggagctcgc cgcatcgtgg gcagtgcgct gttggcgatc cctggcctcc tgatcgcggt
     1201 gcctaggctg gtcgagacgt ggattcggcg cggatcaccg atgtatccct ttccgctgaa
     1261 gctcggaaag tgggtgatct ccgccggtga gagcgaactc gttgccttac tcaccgcgcg
     1321 ctacgccgac aagcgcctcg aggccgccaa gtcagagctc ttccagcttc tgttcgtgcg
     1381 ttcggttcga ccggactggc cgcacgtcaa tttcggttgg ggcggcgcgg cgctcttgct
     1441 cgcgggtgca atcgggctcg gtttttggct gactcggcgc cccaaggatt tgcgggtctg
     1501 gctgttcttg gccttgatct gcaccgcgtt gccctacttc agcggcccga ataccctcgg
     1561 ctatcgtatc tattggtggt ggatcatcgc gcgcttcatg ctcggcgcgc tgttggcgtt
     1621 ggtgttgctg gcgtcggtgg tgcccgggcg ttgggtggac tacgtgtttg cgggttgcag
     1681 cgtcggggga gccgcatacg cgctcggttc gggcatgtgc tcctatcgct ggatcggagc
     1741 ggcgtccgta ttgttctcgg ccacgtgtat tggtggtttg agctggatca ttcgacgctc
     1801 ggggtacccg aagctggcga ctggtctcat cctatcgggg gcgctcgggc tcgccagctt
     1861 actcctcatg ctgcgcaaag agcttgcgta tatcgtactc gccggtgcgc tacgcggtca
     1921 gtgctacgaa ggccatcccc tcacacacgc ggtgctgttt gcgcgtccgg tgtgggacgc
     1981 attggcggac gcgccacccc gacgcatcgc tctcgtgagc ggattcgaag gggtagggca
     2041 caatgtcttc cggtacccgc tgctcggccg tcgttggcaa catcgagtgg tgtacgtggc
     2101 cccgagcgcg gatggcaagc tgcgcggcca tcccgacatc tacggcctgc gtgatcgtcc
     2161 agcgctctcg ttcgaggcct ggctcagccg gctcaaacaa cagcgcgtca cgcacgtggt
     2221 gctggtgccg ccgttcaacg tcgaagagta ccgttgggtg ttcgagcaca agcgccactt
     2281 cgtgctcgaa caacgcggcg agaaggacta cgcctttttg tttcgagtgg tgccgtgagc
     2341 gccgtcacaa gctcgaactc gccgatggcg tcggtttggc gatctgaatg accgggccgt
     2401 gcttcagccg ctcgggatcc gtgcgataag gagccaacgc cggccagagc gcgcgcaggc
     2461 gggccacgcg gcgcatgcgg agcgcatagc tctcggcaaa ctcgggggag ttgccgccgt
     2521 gcgctcgata gcgctcgtac agctcggtga gctgcttggg ggttgccgtg ttgaaggcga
     2581 acaacatcat gtcgttcaga tcgcgaaggg tgatgtcagg ccgttggctt gcgagcagca
     2641 gcgagtcgat ggcctccttg tagcggaagc agcgtgagtc cagcgacgcc tgcgccagga
     2701 gatctcgtgt gttgtccggg ttgaacgggg ggcgttcgcc ctccttcttc aagtcttcgg
     2761 tggtctcctc gaacttcgtg taggcggagt ttttggggaa gagccgcttc gcgatgcgga
     2821 cgtgctcgag ggcgaggtcg aacttgtttt cagccaaata gattgatgcg agcgcgaatt
     2881 ggaacttcgg gatgtggggc gcgcggctca cgacctgacg ctgaacggcc tcggcatcct
     2941 tcgcgcgccc caagaagagc aagcgcacgg cgaggttgtt caaggtcatg gcatagttga
     3001 aatcgtcgga cgtctcggca tccaggtaga cggctgttgc cgctgagtgc atccccgccc
     3061 gcgcgtacac gtttccgagc tcgagcctcg agctcgcgtt gcgcgtgcgt tgctctttga
     3121 aagccgtggt ccagaagtcg atctcgtcgt tccaaacgag gctgcgcgct accgtagtgg
     3181 gcgcgtagct caaagtgcag agcagcacca agatccaggc tggatgccgc agcgaccgat
     3241 cgcgtagtag aggcgccagc aacagcacca gcgcggcaac gggtagatac aggaaccggt
     3301 cagcagccac gacattgacg gtgatcggca cgatgtgcag cacgagaaac acgctgaccg
     3361 cgagcaccgc cagagcggac tggccgctag ggtcgagcac cggccgcccc gcgcggctcc
     3421 gtcgaatcaa ccaggcgacg cccgctacga ttgcgagcac tacgagccca cctagcccga
     3481 cgaagcccca ggtgcggtca tcgagggctc ccactcgggt ggacgggtgc cagccgtcga
     3541 cgagcatcca cgcatagcgc cccagcgcct cggcgcctga cgggaagcga tcggcccagg
     3601 gcaatccatt gggccgaagc gcagcgccca cggcatacgt ccgcaaggcc gtgtacacga
     3661 gcagaattcc gaagaatgga agtaggcgct gcaagcgacg ccaaaccggc aaaccggtag
     3721 cctcgtgcca ctccagcacc aggcaaccca gcacgccggc catggccact tccttggcga
     3781 acaacccgat caacagcgac agagcggcgg cacagcggcg tagccaggat cgagccggcg
     3841 tcaggtacag accaatcaat acgaaggtcg tggcgagcac gtccgtccgc cccgagatcc
     3901 aggctgcagc ctcggtgagg cgcgggaacc acgcccacat gacgctgaga atggcgctgc
     3961 gaatcgcgtc gagcccgcgc ttgcgcagga ggccgaacag caggctggcg ttgcttgcgt
     4021 gaaacaacaa gttggtgacg tggtaacccg ttgggttgtc cccgtgcagc cggtagtcga
     4081 tcgccaagct gagcagagac agggggcgat agtaaatgcg cccatcgacg tcttgctcgc
     4141 tcgtccccca gaacgagctc gtgaggtaga accacaagga tttcagctca cgaagccggc
     4201 tgttctcggt gagcagcgag tgatcgtccc aaatgaagtc accgcccagc gccgagacgt
     4261 aaaccgcgca ggtgagtacg aatacgcacg cgccgactat atagccgcgg atcgaccaag
     4321 gctgggcttc ccgggaaccg gaaacaactt cggactgtcc tacagcttcc aataatgagc
     4381 cctcgtcaat cgcggccgtg accgcgccgc ccaagcacga cagcgccgtt cttcggcgcc
     4441 cacaaagcgg agcagcgcgc cagtggatga aaaccatggc gcgctgccca atctagatcc
     4501 gggtcgaatg agacggagcg tagctcgacc gtgtccgctc ccactcacga tccgaactat
     4561 tcgtcggggt tgatttcgcc gatgttcggc gcgtagatcg cggtttgatt gcccgcgccg
     4621 tccgcggcca caccgccggc gatgtagaac gtcgacgtgg acgtgtcccc gtcgaggttg
     4681 ccgtgcgcga tacagctgaa gctgccacca ccggtggcga cgccagtcgc ttggtagtcg
     4741 tactggtagt actgcggatc gttcatcgag aacctcaggc acatccagcc agcggtgctg
     4801 gttccgctat cccattgact cggggaggac tggaacttct ccgccgacgg cacgcccgtg
     4861 ggaaccgctg tcgcggagtc acacagtttg tgcgagatcc ccgtggaggt gccgagcgcc
     4921 atcacgttgg gatccatatt ctctcgttga taggcaccca ctgcgtcttt cgccattcga
     4981 cccagcgcgt cccgggcctc cgcgctcttg gcgttcttca cgtacttgct cacgccgtaa
     5041 atcgcgagcg cggccaaaat gccgacgatc gcgaccacga tcatgagttc tacgagcgtg
     5101 aagccccgtt tactacgctt gagtttggca atcattgctg gaaaatctcc ttcctcgtca
     5161 tgcttgagaa aacttctgca tgggcgccgg ctcagcgagc cgttcacaca tgtggaatcg
     5221 attgggttca gcgccggtca ttcaccttcg ttcgccgaga agatctgagt cgtgaaactc
     5281 ggtgcgacgt atacggtctc tttgccgccg ctgcttagat cggctttggc tttcactacg
     5341 taccagggcg aaccgagggc cgtcgttggc cagctgccga ccgtaatatc ggtgccgggg
     5401 ttcgtcggcg tgtcgcccac gccgcccgcc gagcaggcat aaacgaacaa caccgggccg
     5461 cctggattta cgcccagggt ggcccagttc tttgcgacat cgtccgttcc gccgccccac
     5521 atcaccttgg tttgtccggg cttggggttc gtcgggtaga agttgctggt gagcgtcttt
     5581 gccgtcgaga cgtcgaggta ctggaacgtt tcgtccttgt acgcttcctg cgcggctttg
     5641 atcgagccga tcagttggat tgcttcgccg gtcttcgagg agctgatgta gcggcgcgtc
     5701 ccatacacac ccagcgtggc cagaatggag acgatcacca cgacgatcat cagctcgacc
     5761 aacgtaaagc cccgttggcc ggcgaacaca tacggcgctg tgcggtccgc tttgcgagcg
     5821 cggcagtccg tgcggcgagc tcggcgcgcg ggtccgtaaa cgttgggcgt tcccattcac
     5881 actcgagcct aagcaactcg catgccctcc ggcaagatct cgagtccgct ttccgcgacg
     5941 acgtcgacga gccgagcggg cgacctaccg tgccgagtcg cgttcgcacc cgcgcggcga
     6001 ttgggccttg gatgatgggg tgtctcggaa ctaaaacacc gcgcgcttgc cggtatgcaa
     6061 gcgcttcggt gacagaaatt gtcactaagc cctgggtcac ggtcaagacg cgtcacgtat
     6121 tccaggctgc tggcctgtag tgccgcgtag aggacagtag ctctgagcca cacggcgtgg
     6181 gccggagacg cggaagtagc tatgctgcgc tttcgatatg cgcgccctga caccgctcga
     6241 agcagcgttc ggaattgccc tggcgggatc ggttctcgcc gtggtgatcc cgtctttcgt
     6301 tcgtaacctt cacgcctctt atgtgagtga ggcgacgacc ggtgttgctg agctggccgc
     6361 gcgaaccgcg gcgcgcctcg aagcggcgca aaccacgagt tcactgccgg aatccgcgcc
     6421 tttgacgcca tccaacgttc cgcggggggt acgcgtacga gatccggagg gcacgtggga
     6481 gcacccgacc tggcgcgcgc tcgagttcaa attttcggtg ccacacgcgt atagcttcgc
     6541 cttcgacgcc gaaagcgcgc ctgacgtcgc caagttcaaa gtcaccgcgc atggcgatct
     6601 ggatggggac gcggtgttga gcacgatcgc catcgacggt acgttcaagc cgggcgccgc
     6661 cccgagcttg tccgcgatgg atgttcaaca tgaaatcgag tagcgctcgt cgcttgggta
     6721 gctggctggc cacggctacg ctcgttgccg gctcgatcgg cgtcattgcg gccttgcgtg
     6781 cgccgctcgc ggccgcccat gcatcggttc agcgccagga agacgtgttc gggttgccac
     6841 gggccgacct gacgcgggtg ctgagcctgg ggtatcgctc cgcgctcgcg gatctacttt
     6901 tcgcacacgt gttggtcgac tacggcctgc acttccaaga caagcgacgc ttcgagttcg
     6961 cggccagcta cctggagacg atcgtggcct tggatccgaa gttccgagag ccttatcgtc
     7021 tggcagacac gctgatcgtg ctgcaaccca cggccccgcg cctcgaggac taccgcgcgg
     7081 cgcgccgcct cggagagcag ggcttgcgag agtttcccta tgacgcggag ctgtggctga
     7141 tcgttgggca gtaccttgcg tacctcgctt ctgcgcacgt gccgtcgaac gaacgcgacg
     7201 agtatcgctt ggagggtgcg cgcaaattgg cccgagcgtg tgagctcgtg agcagcaatc
     7261 agaacatccc gtatcactgc atcaccgcgg cggcgcttct gaatcagatc ggccaacagt
     7321 cggcaatgaa gagcttcatc gagcgcgtga tggctatctc ggacgacgca gaaatccgga
     7381 gcgtcgccgg tggctatctg ggccgtgtgc ttggtcaggt ggagcgagat gcggtggaag
     7441 aaagagggcg ccggctgcgc gctctctggt ccagggattt gaccttcgtc tcgcgcgaga
     7501 tgtggttgct cctcgggcct cgcttcgacc ccgcgatgtg cgcagggtcg ggtcaccacc
     7561 gcgagccagc gtgcgccacc agctttcgcg atctgaccga ggccgagcaa gcttccgcgg
     7621 gcgattgagc tttcgccgaa aggcccgctt cgcgacgcaa agccggccac aaaacgagtc
     7681 gagctggacc gcctcggcag cccagctcgc tcacttcgta gttcgagcgc ctgaccgcgg
     7741 caggcttatg ggaagcgcac cgaaatcccg acggtctttc ctgcttgcac agtgacgctc
     7801 ttggctttcc ggccatgttc tgggtgaacg aagatcaccg tatgagaacc gggcggcacg
     7861 ctcagccccg ctttgggcgt ggcccctagc ggacgcccat ccagaatcac gttcgagacc
     7921 ggattcgaat tcaggttgag cgtcccgttg cccgccgcag gtgcgggctt gggcggcgtg
     7981 accgccgcga tcgcagcggg ggccggtgaa gggctccgcg aaggccccgg tgacccgtgg
     8041 ctcgtcggcg tgttcgcgtt gtccgaggtg gagaccacgg gcggcaccat gtcgatctgg
     8101 aacgtccgct ccgcttgacc gtcttcgaac tcgagctttt gctcgaacgt ttggaagcca
     8161 cgcttgttcg caacgacgct gtatggcttg tccgtcggga tatccaccga cacgggcaga
     8221 ggcggcaccg ggcgccgctc cgttccactc acgagcacga tctgcgcgcc gtcggcgttt
     8281 agacccgact tgaaggtcgc caaacccttc tttactttgg gtttcgcgtc gatcagctcc
     8341 aaccgatcag cagtgacgtt caccgttcgt tcgaaaggct catagcggtc gccttcgatg
     8401 cgaacacggt gctcaccggg cgtgagatcc ggtagctctt ggggtagcgg accgatctcc
     8461 ttgccgtcca cgagcagttt caagccgcgc gcttcggagc gcacattcag cccagagccg
     8521 ccggaacggg tcaggacgat gttcagcacg ggttcatccc cgctcgcgac ttttacagct
     8581 tgatccgccg tcgacgcgta accgggtgcc gtaactttga ccaggtgagt gccggcgctc
     8641 aggtctttca cctggcaagg cgatgccgtg cacttctttt gaccgtcgac caaaacctcg
     8701 aggtcactga cggtgcggct accggggcca gccacggtga cgacgagcga gccgctcttc
     8761 gggacgagta acgcgatgag tccgatgagg acggcggcgc cgcccgctgc ggccgcgatc
     8821 aagaccggct tgcgcacttt acgcaggggg gtgctgatcg tcggttcggc ggcgctactc
     8881 gcgagtggca tcgcaggcgc aggcaacgcg gccggcacag ggggagccga tacacttggc
     8941 agcgcacgcg gcggcgggag cggggcgcgc ggcaaagacg gcgcaggcgg ggcgctcgag
     9001 atccgagagc tcgagatcgt ggtccgcccg agcgaaccaa ctccgggtgg tggaggcggc
     9061 ggacgagtcg cgactggcgg cggggctcct gcggcgggtg ggggaggcgg tgccccgcgc
     9121 agcagcgacc gaacgttatc ttcctggctg cgatcgaaga cggtcgtggc ctcgtcttcg
     9181 tcgtcccagt ccacttcgac gccgccgcct ttgggagctc cgttcagctt gctctcgctc
     9241 ggcagcttgc tttcgctcgt gagcttggct tcgaccggga gcttgctctc gctggtgtgc
     9301 cccatcaccg tggtttcatc ttcgctcgga gctaggtcgc cgttaccgag accgttcaac
     9361 gcgttcaggc cattggagct cgagccgttg gaactcgtga agggcggggg cggtggggga
     9421 accgcaatcg cgccgggggc tggcggaggt gggggcggtg gaacagcgat cgcgctcgga
     9481 gcgggtggag gtggcggggc agcaatggct cctagagacg gtggaggtgg cggcggtacg
     9541 acgagcgctt ggccggtgcc cggacccaac agcaaaggcg cgggggcggc tgccgcgctc
     9601 gggctgggcg gcagggccaa cggcatcggc gtcgagatgt tcagcggtgg cggcgggggc
     9661 accggcgagt tcagcttgct cggtgccggt gggaacggcg ccatcgaacc agtgcggctt
     9721 ccggggggcg gcgggggcgg cggacgacct gtcgtcggag ctcccggcgg cggagctgca
     9781 agaccgagca gcgtcttctg tctggtcatc gagggcggcg cgaccggcac ccgggaacca
     9841 gggggtggag gcacactagt catgccatgc gcaggccgtg atttcttggc ggcgagacca
     9901 tcgaagatgt ccagatccga actggccttg tcggtcattg cagttctctt tctactgtaa
     9961 ctaattcgag gccgagaatc gggctgaggc gctgaattcc ggcaagttca agggctgtcg
    10021 gacaaattct ggggtgtgcc tcgcgcctaa tttcgaccac acaagatact cccccttcgc
    10081 acacttggcg agggggcatg ggcgaattcc actgtgggct gtaatcccca gaattcgcgc
    10141 aagctcaaca ttgtatatcc atgctgtccg agctcggcgg cagctctgtt gagtctgctg
    10201 agtttgctca gtctgctcgg cgtcagcgtc acgtgtgccg agcttgcctt tgcacgagcg
    10261 ttgttcaaga gagtcgccgc tctcgtatgg actaggtcat tcggacgagg ccggcgctgc
    10321 ggggccgatg atcgagatca gccgctgggg gcgtcaatca ctcgccgggc ttgtcggcat
    10381 cgccgcccgg atcctgattg ccggggccac cgggctcatt cgcttcggtc gcacccgatt
    10441 cgggtgcgac cgtcggtggg accgaatcgg tcgcgtcgag cttgacactc ttctgctgca
    10501 gcggagccca ccgttttggc atgacgatcg gagtgctcgg gaaaatcctg cgcacgccgt
    10561 tgccctcgcc gcggaccagg gtaacgcggt ccgtcgaggc cggtacctgc gggtcgtcgg
    10621 gacgcgcaaa atcagccccc aagggccgcg ggagcatcag caccgagtcg cagtgcatgc
    10681 gatgcagtaa agcgccgagc agggccgcgt ctcgtcccgg ttccggccct tcggtgacgc
    10741 gggcgtaaat caacatgccg cccgcgtcga tgccgatggc agcctgcccc cgagcggatt
    10801 cttctgagcc ttcagggaag ccggaagtaa ccttctccgc tcgcgaatcg ggtggtttgt
    10861 tgccgattac gaatccggtg gtctcggagt gccagagtgc ggcgggtcct tcggcaacga
    10921 cagatctgaa gtccaagatg cgttgcccgg cgacgtcccc gggcttcgct acccgcacca
    10981 cgcgcggatc gatttttatc aagccgactc gcgcgaacgg gcgactcgct tcgggacgga
    11041 cattggtggt ggcaaccgcg tagggccagc cgtgctgcgg cagaccttgg gtgcgccagg
    11101 cgccctcgcc ctcaccgggg atcgcggtcg gggcgggatc gccgggcaac acgtggcgca
    11161 gcgtgagata gaagaagtcg cgcccttccg tgttgatgta gcgagggaaa ttcatgagcg
    11221 ccatgaaccg aatcatgcgc ctactcatga actcccatcc tggtttgccg ggcacggggc
    11281 ccgccgcttc ccagacgtcg tccaagcgac gacccaaatt gggcaagctg cccttcggac
    11341 cgactcgata gaactcgaga cccgtgtggc ccgcattcat gtcgaggtgc acaccgtacg
    11401 tgcagcgcgc cttcttcatt gacagcgcga ggacgtctgg gtccaggttg gctccgtaga
    11461 agtaccctac gaagccttct ttggtcatgc acagcgcgct gcgcacggta cggctctctt
    11521 ccgtccaacc cggcggaact ccgccccacc aatgccgctt gtacggattc ggcacctcgt
    11581 ccacgatcag gggcgtcatg ttctggcgaa acgagacgat ggcttcggga acgcggctat
    11641 cctccggcca ggttccgaag cccgtcgcac catccgaaag cttcgccacg gtggcaccat
    11701 agggctttgg cggaaggtac aagacgcgat ccgccatcat gccgaactcg ccatgaatgg
    11761 cctggaaacc accgttgaag gcggccacga agcgcccgaa cgttcgatcg ttgcggggca
    11821 ccatgccggt gccggtctca ccggtggcgc tcttcggctc caccgttcca gacaccatgt
    11881 tgagctcgac ctggcgcgga tcccacaacg tgatgaacac ctgtgagtag cgccgctttt
    11941 tgtctgcgcg gatgaaggtg tacacgaacg gcgcgggggc cccgacgttt ctcaaaacga
    12001 acgggtcgtt attcagcgcc acccacttgc cctctccctt gaggggggga tccagcattg
    12061 ggaccagtgg aggcggcggc cagcccgttt cggcgttggt cccgtcgctc gatggtgccg
    12121 cgtagagatc gcctaactcc tccgcgacgc tctctgcgcc atcatcgccc gtgacggtcc
    12181 cgacgatctg ctcgaattgg tccacgccct ggaaggcgac ggccttgagc agttgcagct
    12241 tcgtgtcacc gatccagggc agggcacgaa cccgatcgac ggcccaagtg accaggtttc
    12301 cggggcgcgc tttggcgggg gtctgatcat gtacgtaacg cgcgccctcg atcgggccgc
    12361 ccccgagggg gatgcggatc ttgtgcgcgt cggcatcgac caacaaggcg cccttcgaga
    12421 agcccaacac gactttgtag gcgggcggat cgagcttgaa cgagcgccgt ccgatgccgg
    12481 aggtttgacc tgtctcctgg tagttggtga gcgcgttttg cagccgagat cgcttgctcc
    12541 agccaggtcc gttcggcacc ggctcgccgc gcatggtcgc gtattgaacg ctggagatgg
    12601 caccctcttg ggcgatcatc cacgcggcgt cgtcaccttg aacgaccagc ccctgctcgt
    12661 ccgccgcgga cgtgctggtc aagttgtaaa gacccgcaac gtcgatcaga tgcccatccg
    12721 gagaacggcg cgtgtccacc aggtagatat cgcggggctc tccacggcgg cgcgcgacga
    12781 cgatcgctcg ctcgcgcacc accggagagc gcacccaggg ctgggttgga gagacgaagt
    12841 ggatgtcctc gaccttggcc gcgagcccca tcgattccaa ggcggcgccg acggcgcgtt
    12901 ctcgattggt ttcgctcagc acgcggtccg gcaggctgtc cccgcgatgg accacgtacg
    12961 caatcgcgaa gaacaacggc aaggggcgcg cgctttccag cagatggcgc cattcgtggg
    13021 ccgcaccttc gcttcgtacg gcggctaccg gtgcggagga gacgtcgccc ggggcggctt
    13081 cacccattcg gtcctcgagc ggtccccaac agggctccat ccagcgacat agtcggtctc
    13141 gcggttaaca tctcgagagc tcgaaggcct atttttcgtc agtttcaggc ggaggggaag
    13201 aaccactccg cgggccccgc gagaattcgg tcgggatgga tgctaaaagt aggccacgcc
    13261 catgtcgatg gtcgctcgcc gtagctttgc tctttttgga gggctgttcg tggcggcatg
    13321 cctgtcaccc acattgcctc tgcctccacc gtcagagccg gaggtgagcg ggccgaccga
    13381 gcaagggctg atccgcctga cgggcaatgc gcccggaggc gcatggatgt acgcgctgaa
    13441 caagacttcg aacgtcgggg ttttcaatcg cgccactccg gaggggcgct acgacttgga
    13501 gatccccgca gaggttgggc atcgtatcgc gctttggtac gagttggacg gtgagcaaag
    13561 tgaatcactg ctgttcaccg tgcgcaccag accgtgacac ccctagtcca acaaacgagc
    13621 ttcccacagg ggctcgatct tcgtgggacc ctgggaaggc atgccgtttg gaaggcatgc
    13681 cgttacgagg gcatgccgtt acgaaggcat ggtgcggagc gcgacccgtc ggccatcact
    13741 gctgcgatgg gacagagcgg gacgcatcgg cactacgggc gcggagatca ccggcggggt
    13801 cgcgtcgtcg acctcaccca acaggtcgta gtcggcgacc tgagtgattc gaacccggtg
    13861 aacttccccc gctcgcactt ctccgcctga aagcaccacg cctccgtcga tctcgggggc
    13921 ttgtccggcg tgtcgcccca ccatcaccag ctcgaactcc tcgctcgggc cttccaccag
    13981 tacctcgagc tcacgcccga tcagcgcttt gagcttgttc tggctaatct gctcctgcag
    14041 tcggctgagc ttgcgagcgc gcgagcgtgc ggtcttctcg ggcaccttgt ggtcgagttc
    14101 gaatgaacga gccgtcggct cgtccgagta acagaacacg ccaagccgat cgaactgcgc
    14161 gaactcgacg aatttcagga gctcgtcgaa ttcttgctgg gtctcgccag ggtgacccac
    14221 gatgaaggcg ctgcggaacg tgagatcggg gatcttggtt cgcaggcgat cgacgagctc
    14281 gtacaagcgt tgcccgccgt gtccgcgtcg catgcgccgc agcatgtcgt ccgccgcatg
    14341 ctggagcggc atgtcgacgt acggcacaac gcgcggatgc tgccccaaga gctcgatcag
    14401 ctcgtcgtcg agcttctcgg gatagaggta gaacaagcgc acccagtgca ccccgggtac
    14461 ctcggcgacc ctgcggacta ggctcgcgag cgcgcccttc gccccctcct gatcacgtcc
    14521 gtacgcaacc gtgtcctgtg agatcaaatt gatctcgatc actccggccg cgaccaaacg
    14581 ctcgacttcc gcgatcacgt catccggcgt tcgcgagcgc tgcttgcctc gaatctgcgg
    14641 gatgacgcag aaagaacacg tgcgattgca gccctcggca atcttcacgt aagcgcttcg
    14701 tccgcgcgtc gacaggcgcc gtggatcctg ggcgcccacc aaccaatcgg caggattgcc
    14761 caccgcgacg cgcggagcgg ctccgcccag cacccgttcg agcgcgagca tgtcgctcga
    14821 acccaccagg ttgtcgatct ccggcagccc ttgcgacagc tcctcggggt atcgctgact
    14881 caagcagccc gtgacgacca gcctttggca acgcccctct tgcttgtagg cggagagctc
    14941 taaaatcgtg tcgatcgact ctttcttggc cgcatcgatg aagccacacg tgttgacgac
    15001 gatgacctcc gcattcttcg gatcatcgac gtgggcgtag ccggagcgtt cggcgacacc
    15061 cagcatgacc tccgtgtcta cgcggttctt cgggcatccc aagctcacga agtgaatggt
    15121 ggggccctcg ggcgcggccg tttcgtccgc ggcggtctcg tccgcggcgg tcggttccgt
    15181 cgtttcggtg gcttcgagat cgatgggcat cagcgcgggc tcagcatcaa atttcggaag
    15241 cgctcgaaca ggtacagcga gtcgtgcgga ccggctgccg cttcggggtg gtactgcacg
    15301 ctgaacgcct tcaggtcggg cgcttcgagg ccttcgctcg tgccgtcgtt caagtgcagg
    15361 tgagtagttt gggccacgcc cttcagcgag tccacatcga cgacgaagcc gtgattttga
    15421 gtggtgattt ccacgcgacc cgtggtcagg tccttgaccg gctgattcag cccgcgatgg
    15481 ccgaacttca gcttgtaagt ccgggcaccg agagcgagcg agagcagctg atgccccagg
    15541 cagatgccga agatcggctt cttgcccacc aagcccttga tggtatcgat cgcgtaggtg
    15601 accgcggccg ggtcgccggg gccgttggac aggaagactc cgtccgggct cagagctagg
    15661 atctccgccg cagacgtgtt cgcgggtacc gcggtgacgt cgcagccgat atcgacgagg
    15721 caacggaaga tgttgcgctt ggccccgaaa tccatggcaa ccaccttcgg gcgttccccc
    15781 gagccggccc gctgcatggg catgggccag gcgccgagtg actcgctgaa ggcgtagcgc
    15841 tcgcgaggag acactcgggc caccagatcc aagccttcca tactgggaac ggtgcgggcg
    15901 cgctcgatca gggcctccag cggcccggca ccaatcacgc cgttttggga gccgcggtcg
    15961 cgcaggtggc gcgtcagctt gcgcgtgtcg atctccgaga ttccgacgat gccatggcgc
    16021 gctaggtacg cgtcgagcgt ctcctgtgag cgccagttcg agaccaccgc gctgttgtca
    16081 cgcaccacga agccggcgac ttgcggccgg tccgtgacgc tctccgcgtc ttcggtgttg
    16141 atgccggtgt tgccgatttg cggcgccgtc atggtgacga tctgcccgca gtatgacgga
    16201 tcggtgagga cctcctgata gcccgtcatt ccggtcgtaa acaccacctc gccggtggcc
    16261 acgccgtccg ccccgaatac gcagccgggg aatacggttc cgtcggctag caccaaatgc
    16321 ccgggtttct gggtcatcgg ctgctcccga gtgcgtcgaa cacgatctta ccgcgcgcca
    16381 gggtcatcaa tattcttcct ttcaactcgc gatccataaa tggcgtattc cggctcttgc
    16441 tctgaagttg catttgtcgc ggagtggata ccgcctcggg gtccaccaag cagagttcgg
    16501 ctagcgctcc ctcgcgcagc ttgggtgctt cgagcccagc aatccgagcc ggccgcgtcg
    16561 acagcgcctc gattaggcgg cccagcgtaa gcccgtcctg accaaccagt ccgagcagca
    16621 ggccgaagca cagctcgagg cccatcatgc ccggtgaggc ggcggagaac tcgcagtctt
    16681 tctcgagcgg ggagtggggc gcgtgatcgg tcgcaatagc gtcgatcgtg ccgtcggcga
    16741 gggcgcggcg gagggctgcg atatcttccg gctcgcgcag cggtgggttc actttacacg
    16801 cggtgtcgta gccaatcacg ctcgcgtccg tgagtaacaa gtgatgcgga gtcacctcgc
    16861 acgtcacgtc gatgccgcgg cccttggctt cgcgcaaaat gcgcaccgct cccaacgtgg
    16921 agacgtgcgc cacgtgatag cgagccttga cgtactccgc caaaatcacg tctcgcgcta
    16981 cgatgacgtc ctcggccgcg cgtggccagc cgcgcaggcc gagcctggcc gagactgccc
    17041 cctc
//
LOCUS       DASTKU010000824        12535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1725342, whole genome shotgun sequence.
ACCESSION   DASTKU010000824 DASTKU010000000
VERSION     DASTKU010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12535
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725342"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VFQ61_28820"
     CDS             <1..452
                     /locus_tag="VFQ61_28820"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET9958545.1"
                     /translation="YNVAALGDGKGQLCGAVAMRKTIVTRLGKAWGAMTVHDPSVLQV
                     AEKIVRGLEWRGGCEIELLKENSGAIHLIEVNPRFPAWIYLSTASGVNLPYGLLRMAL
                     GKEVPRFEPHQAGVFYVRHASEAIGNISDLEHIVHQGRKSALVSAGL"
     gene            502..1854
                     /locus_tag="VFQ61_28825"
     CDS             502..1854
                     /locus_tag="VFQ61_28825"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET9958546.1"
                     /translation="MQTQIYERPVIVRNPVGLMNKIGRRGDVRSQREIDGVPIQELCQ
                     SFGSPLFVFSERRLRSRIAEERAAFAKYPGTRLCWSYKTNYLSALCAICHQEGSLAEV
                     VSGMEYEKARALGVPGSSIVFNGPSKTRNELELAAAEGAILHADHLEEIELIERIAIE
                     THTTPQIGIRVGLDAGIYPRWDRFGFDLESGLAKEAVRRILRSGRLKFGGLHSHIGTF
                     VLDAAAYGRAARKVLEFASVLEGELGVRPTHLDMGGGFASNNTLGGQYHSGALVPPIG
                     EYAEEIVTALTEHKGPRYPLLVETGRALVDDAGTLITSVLASKRLSTGKRAIVVDAGV
                     NLLFTAWWYRLGISPTRAVDSFLEDTAVYGPLCMNIDCVRESIPLPELRAGDALSIHP
                     AGAYTFTQSMQFIKLRPACVLINRSGDVELIRRAEELDDLTRPELLPDRLRGPYSEIQ
                     "
     gene            1858..4131
                     /locus_tag="VFQ61_28830"
     CDS             1858..4131
                     /locus_tag="VFQ61_28830"
                     /inference="COORDINATES: protein motif:HMM:NF015226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HET9958547.1"
                     /translation="MDARQRQLGPRIAAAGKTVLRCTLSSYASVVFSRSQWSGVLLFL
                     ASLQHPMRAAAGLLATLVANAFAAAMGYSKERIQSGYYGYNALLFGLFLVHESPLSTF
                     LVLGLLAGAVVSAMLSAGLGDALAMLGLPLLPLPFVILASLLGHTTLELAVVPPWLTT
                     ASDLPPTLPLAETSIFGVSKCMLEGLGAIAFAPTGLAGGLVLAAALVYSRITGLGLIF
                     GALLGQALLAVLGRADANLALPVQYNAALTFAAVASVFRLPSLSTLGLAAFATWFGAW
                     LTTAWLVPFAPVSLPVLAWPFVLTTLLVLRALASAPARASLAPLLLPHGSPEANLNYR
                     DMLAERLGVPGPARFWLPVEGHWTITQGEHGLLTHREPWAHALDFEILDGQGFPFRGD
                     GTRLEDYYCFGQPALAAGTGTVVFAEDTQPDNQPGHQDLQLPYGNVVIIQHGEGLYSV
                     LAHLRRGSLTVKAGDFVVVGQVVGACGASGRSPRPHLHFQVQASPWLGAPTIPFGITH
                     YTARHREPTLALPAVSQAERLHRLGVPREGETVRQLRPDAPATAGVPAFPGRGFELSM
                     SSGACCSVRSEVAWNGEQSLVSEDGKARLYFVQNQLGLVFTTYQGSWHHPLGILFQCL
                     PALPRGSSARVAFSETLPASSFMPPGLRVIYDFFRVFGQLVSIRVDCECVHLPEPAVG
                     LVVTSEIDWRVLGRSVRRRIGRVEFDALGIISVESWTSRRLSRFSARRIPATFPVEAS
                     LGNATGSNFEKVNSIHE"
     gene            4124..4762
                     /locus_tag="VFQ61_28835"
     CDS             4124..4762
                     /locus_tag="VFQ61_28835"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9958548.1"
                     /translation="MNSASMSWWASLWIGCSAAVAMTSGLAFAADSEDTLLVSYQLEA
                     KGDLAGALRASHKLADKNPKQYFARLRAAYLALAAKNFVQAARDYEIAAALAPKAIEP
                     LLGQQQALMALGKYGEAEPIGRAAVERDPLNYLANSRLAWTLFSLKKYAEAARVYSRV
                     LALYPGDIEMAVGLGYAELRSGQKQRAQETFRAVLGMAPKHVRATQGLAEAR"
     gene            4926..5762
                     /locus_tag="VFQ61_28840"
     CDS             4926..5762
                     /locus_tag="VFQ61_28840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958549.1"
                     /translation="MVMSRGSESLRARVAGLAIATALLAAAPAGAQVLPGLVQPGEPD
                     RIGLPDLAGLQGSADWQEPGTTPVLFGNQRAPNEDAGTKKPGERLRFGPVLGAGFPNV
                     ISVGATLKINQYVGAGVNWGMIPEMGAPGIESTTLTYREYDVYGRVYPFGDALFAGLG
                     VGYEEISTATAQTLQLRSSAGSASLNVSGEASVKSLILIPQIGIFHTFESGFSLGSEV
                     GVQLPISASDVTLTEDPAPTAAQRAAIPALGQTEDKVVNTLRSYSKTPIPTIGFRVGW
                     LL"
     gene            complement(5850..6044)
                     /locus_tag="VFQ61_28845"
     CDS             complement(5850..6044)
                     /locus_tag="VFQ61_28845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958550.1"
                     /translation="MPSVLAPAASEKASTLAVMTAGTGPPRAGAGAHLTSDICPGAKA
                     PNAWVRAFLLRPPNNVRDTR"
     gene            6047..7321
                     /locus_tag="VFQ61_28850"
     CDS             6047..7321
                     /locus_tag="VFQ61_28850"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9958551.1"
                     /translation="MTLEEWNKRSESLRGAKVAGVFELETLIGVGSMGAVFRARDGSG
                     RPVALKLLHPELMASDAARRFEREADILLSMRHPNLVETLAAGHDSKLDLLYIAMPLL
                     QGEDLDTVLARRGALPENIALRLAVQAGRALAAAHAASVVHRDVKPGNLYLEQGLGNE
                     VILRVCDFGIAKRLLGGPQTPLTATGTQLGTPDYVAPEQLRDSKNVDPRVDVWGLGAT
                     LYEMLSGEPPFGHHQNVFDVIGAILSEPVPSLAERAPWLREEVVATVHEALAKKPEER
                     FGSMSDFLAALESLVASPFTVTRADLALPSPKRPTAAGSTIPGSEPSKPPDADLPELI
                     PVESVRSEVAPIRASVSATAPSSLAPSASATSSDRSAKRGGAAAKHGGSAASKNKGPE
                     APRRAFWVGVVLLLSSALVSYWVLRFLLSMRA"
     gene            complement(7282..7971)
                     /locus_tag="VFQ61_28855"
     CDS             complement(7282..7971)
                     /locus_tag="VFQ61_28855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958552.1"
                     /translation="MATSSFSPVCPSCGRRAPIVLRTESGAPGARCVACGAARLPLVA
                     PSVTFAGQPSRVGGIAASFFGVLVLVFGLTLAVSVFLLLHSIWPASLVGWAFALPITI
                     ASLFFGMLLLFGGHKLRKSGAQKSHAVRLDAARNLLAHRGSLDAREAAQAIGISAEEA
                     DALLTELAKDAGQDVSLEVADDGQLRYIRGSAQEARFRVLEQQFGTQPGSPDFAAEAQ
                     ARIDSKNRNTQ"
     gene            8157..9128
                     /locus_tag="VFQ61_28860"
     CDS             8157..9128
                     /locus_tag="VFQ61_28860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9958553.1"
                     /translation="MPLLSIEDLRVEFTTESGSVTALCGVDLALEAGQFAALVGESGS
                     GKSVTALAVLGLLGPNARRRARKLEFDGQDLLKASASALRELRGRQIGLVFQDAMSAL
                     NPVSRVGDQLTEVLVLHRGLSRHDAQTEAVRALGRVGIAGPEQRARQYAHELSGGMKQ
                     RVMIALALAGQPKLLIADEPTTALDVTIQAQILTLLKTLQAELGLAVLLITHDAGVVA
                     QFAHRVHVMYAGRVVERGPTAKVWGAPGHPYTRALLAALPRPEFVRRHLPAIPGQAPV
                     LTEPLQGCAFSPRCAEALPQCRRELPRPREIAPEHETSCWIPPRQPG"
     gene            9125..10210
                     /locus_tag="VFQ61_28865"
     CDS             9125..10210
                     /locus_tag="VFQ61_28865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET9958554.1"
                     /translation="MIDAPMKEVAPFPSRALLEVENLSKDFPVRDVLGRSRGSVRAVS
                     NVSFRIQRGETLGLVGESGCGKSTIGRAVLGLLEPTHGHVRFDGVDVARASRTELIAL
                     RRRMQIVFQDPHASLNPRLSVWAALAEPLEVHGVTRGAETSQRVTRLLDRVGLPDSVK
                     KRFPHELSGGQCQRVSIARALSLGPELIVCDEATSALDMSVQAQILNLLLDLQRELQV
                     AYLFITHNLAVVRHLADEVLVMYLGKVVEQASAAQLFAAPMHPYTRALLAANPSSDPK
                     VSFQPEPLTGELPSPLDPPAGCAFHPRCRHAFELCRTRVPALRAPDPRPLAEAKNAGT
                     QTDTAAVQTNVTGRTSRVACHLYDDAP"
     gene            10200..12206
                     /locus_tag="VFQ61_28870"
     CDS             10200..12206
                     /locus_tag="VFQ61_28870"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9958555.1"
                     /translation="MLRSWLYFIGFLALGLLLVGLTFSAASLERADFAFVNLTEPKTL
                     DPGAMTGEPEGRLADALFEGLTRLDGQTLESAPGVAESWKISADGTRYEFTLRPNARW
                     SDGRPLTAHDVTFAWRRLVNPRFGSEYAYLLNMVKFAEAFNSFEAQSKALERAAAALE
                     ERADPSGRLARATFLELAKKLGLPPIFAPMDDRALSDWLRHPEWIEQPGGVREFTTAL
                     RNESRRRLERYRDAERHFGIDAGVFATDDHHVTVELVAPTPYFLELTAFYPTYPLPRH
                     AIEKFGDERWFLPGHIVSNGAFVLDQWRVGDRIRMLRSPTYWDRGRVKLRSVDALPSE
                     NATTSLNLFLSGEVDWLPTNSYPVDLSEYLAQRPDYFRSSALSVYFYRINCTRKPFDD
                     PRVRLAINLAVDREVIAKQVVAQGQIPAYRVVGPGIPGYPEAETPLRYDVAQARKLLA
                     EAGFPDGRGFPKFGVLYNTMDMHRKIAEVIADQLRRNLGIQATAYNQEWQSYLTSYRA
                     LDYDLSRAGWVPDYLDPNTFLDLWVTDGGNNSTGFSDATYDELIRAASNVERFLKAPE
                     LRGGTFSHPERIERAVQAVRDAATPDQHLAQAVALRMELLREAETRLFSQGPPILPIY
                     FYVNSGMINPKVRGFHWRIPDGKGGWRSNVRDRHPISELSVDGR"
     gene            12196..>12535
                     /locus_tag="VFQ61_28875"
     CDS             12196..>12535
                     /locus_tag="VFQ61_28875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958556.1"
                     /translation="MAASTLITVAALAALLVGSAYLPKSSVRWLGAAAFGTLFAVFAR
                     FALGWPVALLGGALLFGVAARWAPELVLRRITFLVPALLLLVFATTLLMFAAPGNPFT
                     GERAMSPSVEA"
BASE COUNT         2110 a         3810 c         4150 g         2465 t
ORIGIN      
        1 aatacaatgt tgccgcgctc ggggacggga agggtcagct gtgcggcgcc gtcgccatgc
       61 gcaagacgat cgttacccga ctcggtaaag cttggggcgc catgacggtg cacgatccaa
      121 gcgtgttgca ggtggccgag aagatcgtgc gcggcctcga gtggcgcggc ggttgcgaga
      181 tcgagctgct gaaagagaac tccggtgcga tccacctcat cgaagtcaat ccccgctttc
      241 cggcttggat ttacctgagc acggcctcgg gggtgaacct gccgtacggg ctactgcgca
      301 tggcgctcgg taaagaggtg ccgcgtttcg agccgcacca ggcgggcgtg ttctacgtac
      361 ggcatgccag cgaagcgatt ggcaacatct ccgatctcga gcacatcgtg catcaaggac
      421 gcaagtcggc cctggtgagc gccggattgt gaaaagactt acgccgcact aacgcgagtg
      481 tggcgcccag cgaggcaata catgcaaacc cagatttacg agcggccggt gatcgtccgc
      541 aacccagtcg gactcatgaa caagattggc cgccgcggtg acgttcgctc tcagcgcgaa
      601 atcgacggcg ttccaatcca agaactgtgt caatccttcg gttcaccgct gttcgtgttc
      661 tcggagcggc gcctgcgcag ccgaattgcc gaggaacgag cggcgttcgc gaagtaccct
      721 ggcacgcggc tgtgttggtc ctacaagacc aactatctgt ccgctttgtg cgcgatttgc
      781 caccaagagg gtagcctcgc ggaggtcgtc tcggggatgg agtacgagaa ggcgagggcg
      841 ctcggggtgc cggggtcatc gatcgtcttc aatgggccat ccaagacgcg gaacgagctc
      901 gagctcgccg ccgccgaggg cgcgattctg cacgcggacc atctggaaga gatcgaactg
      961 atcgagcgga tcgcgatcga gacacacacc acgcctcaaa tcggcattcg agtcgggctc
     1021 gacgccggta tctatccgcg ctgggatcgc ttcggcttcg acttggaatc gggactggcc
     1081 aaagaggccg tgcgccggat tctgcgcagc ggtcgactca aattcggcgg ccttcattcg
     1141 cacattggaa ccttcgtgtt ggacgcggcg gcgtatgggc gcgcggcgcg caaagtatta
     1201 gagtttgcca gcgtgctgga gggtgagttg ggggtgcgcc cgacccacct cgatatgggt
     1261 ggtggctttg cttccaacaa caccttgggc ggccaatacc attccggagc cctcgtgcct
     1321 ccaatcgggg aatacgcgga ggagatcgtg accgcgctca ccgagcacaa ggggccgcgt
     1381 tacccgctgc tcgtcgagac gggccgggct ctagtcgacg atgccggtac cctgattact
     1441 tcggtgctgg cgagcaagcg attgtctacg ggcaaacgcg cgatcgtggt cgacgcaggg
     1501 gtgaacctgc tgtttacggc ttggtggtat cggctcggga tatctccgac acgcgcggtc
     1561 gactcgtttc tcgaagacac tgccgtctat ggcccgctgt gcatgaacat cgattgcgtt
     1621 cgcgagagta ttccgctgcc cgagctgcgc gccggtgacg cgctgtcgat tcacccggcg
     1681 ggcgcgtaca cgttcacgca gtcgatgcag ttcatcaagc ttcgccccgc ttgcgtgtta
     1741 atcaatcgat ccggtgacgt agagttgatt cgccgtgcgg aagagctcga tgatttgacg
     1801 cgccccgagc tgctaccaga ccggttgcgc ggtccctact ccgaaattca gtaagtaatg
     1861 gatgcacggc aacggcagct agggccgcgc atcgcggccg cgggaaagac agtgctccgg
     1921 tgcacgttga gctcctatgc cagcgtggtc ttctcccgca gtcagtggtc cggcgtgctg
     1981 cttttcctag cgagcctaca gcacccgatg cgagcagccg cagggctgct cgcgacccta
     2041 gtggcgaacg ccttcgcggc ggcgatgggc tattcgaagg aacggataca gtccggttat
     2101 tacgggtaca acgcactctt gttcgggttg ttcttggtcc acgaaagtcc gctctctacg
     2161 ttcctcgtac tcggcttgtt ggcaggcgcg gtcgtgagcg cgatgttgag tgcgggcctg
     2221 ggggacgcct tggcgatgct cgggctgccg ctgttaccgt tgccgttcgt gatcctcgcc
     2281 agcttgttgg ggcacaccac actcgagcta gcggttgtcc cgccctggct gacgacagct
     2341 agcgatttgc cgcccacgct accgcttgcc gaaacgtcaa tcttcggagt ttcgaagtgt
     2401 atgttggagg gccttggggc gattgcattt gctccgacgg gcctggctgg agggctggtc
     2461 ctagcggcgg ctttggtcta ctcccgcatc actggtttgg ggctgatttt cggcgctctg
     2521 ctcggtcagg cactgctcgc ggtgcttggc cgcgccgacg caaacctcgc tttacccgtg
     2581 cagtacaacg ccgcgctcac gtttgcggcc gtagccagtg tgttccggtt gccctcgctt
     2641 tcgactctgg ggctcgcggc ttttgcaacg tggttcggag cctggttgac gacggcttgg
     2701 ctcgtgccct ttgcgccggt ttcactaccc gttttggcat ggccgttcgt gttgacgacg
     2761 ctgctggtgc tccgcgcgct cgcgagtgct ccggctcgcg ctagcctcgc tccgctgttg
     2821 cttccgcatg gctccccgga ggccaacctg aattatcgag acatgctggc ggagcgcctc
     2881 ggagtgcccg ggcctgctcg tttctggttg ccggtcgaag gccactggac catcacgcag
     2941 ggcgaacacg gtcttctcac tcaccgcgag ccctgggcgc acgcgctcga cttcgaaatt
     3001 ctggacggcc aaggctttcc ctttcggggc gacgggacgc ggctcgagga ctactattgc
     3061 ttcgggcaac ccgcgctcgc ggccggaacg ggtaccgtag tatttgcgga agatacccag
     3121 cccgataatc agccggggca tcaggatttg cagctgccct acggcaacgt cgtcatcatt
     3181 caacacggcg agggtctcta ttccgtgttg gctcacctac ggcgcggctc cctgacggtc
     3241 aaagcgggag acttcgtggt ggtgggtcaa gtggttggcg cgtgcggggc ttcgggtcgc
     3301 agtcctcgcc cacacttgca ctttcaggtg caggcgagcc cttggctcgg agctccgacg
     3361 attccatttg gcatcacgca ttacacggct cgccatcgcg agcctacgct cgcgctgcca
     3421 gcggtgtcgc aagcagagcg cttgcaccga ttgggcgtcc ctcgcgaggg tgagacggtc
     3481 cgtcagttgc gtcccgatgc tcccgccaca gccggcgtgc cggctttccc gggccgaggt
     3541 ttcgagctca gcatgagttc cggagcctgt tgctcggtgc gcagcgaagt ggcttggaac
     3601 ggcgagcagt cattggtgag tgaagacggc aaggcacggc tatacttcgt tcaaaatcag
     3661 ctcgggctcg tcttcacgac ctatcaaggt agctggcatc accctctagg aatactgttt
     3721 caatgcttgc cggctttgcc gcgcggctcg agcgcgcggg tcgcgttctc cgaaactttg
     3781 ccggcctcga gtttcatgcc ccccggcctc cgggtgatct acgatttctt ccgcgtcttc
     3841 ggccagctcg tctcgattcg agtggactgt gaatgcgttc atttgccgga gccggcagtg
     3901 gggctggtcg tcaccagcga aatcgactgg cgtgtcctgg ggcggagcgt acgccgccgg
     3961 atcggacgag tggagttcga tgcactgggg atcatcagcg ttgagtcttg gacatcgcga
     4021 cggctctcac gatttagcgc gcgtcgcatc ccggccacat ttcccgtcga agcaagcctg
     4081 gggaatgcga cgggctcgaa cttcgagaaa gtgaactcta tacatgaata gtgcatccat
     4141 gtcttggtgg gcctcattgt ggatcgggtg tagtgccgcc gtcgcgatga cctccgggct
     4201 agccttcgcg gcggactccg aagacacgct cttggtcagc tatcaactcg aagcgaaagg
     4261 tgacttggcc ggtgcgctgc gcgcgagcca caagctcgcc gacaagaacc ccaaacagta
     4321 cttcgcgcgg ctgcgtgctg cgtacctggc cttggcggcc aaaaacttcg tgcaagccgc
     4381 gcgcgactac gaaatcgcgg ccgcgcttgc accgaaagcc atcgagcctc tactcggaca
     4441 acaacaggca ctcatggcac tcggcaagta cggcgaagca gagccgatcg gtcgcgccgc
     4501 ggtggaacgg gacccgctca actatctcgc caactcgcgt ttggcttgga cgctgttcag
     4561 cttgaagaag tacgctgagg cggccagggt ctattcgcgt gtcttggcgc tgtatccagg
     4621 agacatcgaa atggcggtag gcctgggcta cgcagaactc cgctctggac agaaacaacg
     4681 cgcgcaagag acttttcgcg ccgtgctcgg catggccccg aagcacgtgc gtgcgacaca
     4741 aggattggcc gaggcgagat gatggccaaa acagagccgg gttagtgaaa ggttttagga
     4801 cgaggttgtt tgtgtttcgg gggcttgaac aaatcgatga ttggctacat ctcatagtga
     4861 gtgactgacc tgcgtagtgc tcacttgctc gtgtgccgct cctgccccgg gagggtgaac
     4921 actccatggt gatgtctcga ggcagcgagt cattgcgggc tcgcgtggcg gggcttgcca
     4981 tagctaccgc cctgctcgct gcagctccgg ccggtgccca ggttttgccg ggcttggttc
     5041 aacccggcga gcctgaccgg atcggcctgc ccgaccttgc tggtttgcag ggcagcgcgg
     5101 actggcagga gcccgggacc acgcccgtcc ttttcggcaa tcagcgcgcg cccaatgagg
     5161 acgcgggcac caagaagcct ggtgagcgtc tgcgcttcgg gccggtgttg ggcgccgggt
     5221 ttccgaatgt catttcggtc ggggcgactt tgaagatcaa ccagtacgtt ggcgcgggcg
     5281 tgaattgggg catgatcccc gagatgggag cgcccggcat cgagtctacg accctgacgt
     5341 atagggaata cgacgtgtac ggacgcgtct atcccttcgg ggatgctttg ttcgccgggc
     5401 tcggcgtggg ttatgaggaa atctccacgg cgacagcgca aacgctccaa ctccgttcca
     5461 gcgcgggcag cgcttcgctg aacgtcagcg gcgaggcctc cgtgaagtcg ctgattttga
     5521 tcccgcagat tgggattttc cacaccttcg aatccgggtt cagtctcggg tccgaggtcg
     5581 gggtgcaatt gcccatatcg gcttcggacg tgaccctgac ggaagatccg gcgcccacgg
     5641 ccgcgcagcg cgcggccatc ccggccttgg gtcaaaccga agacaaagtg gtgaacacgt
     5701 tgcggtcgta ttccaagact ccgatcccga cgatcgggtt ccgcgtcggt tggctcctgt
     5761 agcgcggagc gctcgcgaag ttcgagtgac gacgggcagg cgcggtgcag cggggcagtt
     5821 gatggcaagg actgcgctgg cgcgagcaat caacgggtgt cgcgcacgtt attgggcggc
     5881 cgcaaaagaa acgctcgaac ccaagcattg ggcgctttcg ccccggggca gatgtcgctc
     5941 gtcaaatgag cgccggcgcc cgcccgcggg ggtcccgtac ccgcggtcat gacggcgaga
     6001 gtcgacgcct tttcggatgc ggccggcgcg agtacgctcg gcatgcgtga cccttgaaga
     6061 gtggaacaag cgttcggaga gcctgcgtgg ggctaaggtg gctggcgtct tcgagttaga
     6121 gaccttgatt ggcgtcggtt cgatgggggc ggtgttccgc gcacgtgatg gcagcggtcg
     6181 accggtggcg ttgaagctgc tgcacccgga gttgatggcg agcgatgcgg cgcgacgctt
     6241 cgaacgagaa gcggacattc tgctcagcat gcggcacccg aacttggtgg agacgctggc
     6301 ggcggggcac gactcgaagt tggatttgct ttacatcgcc atgccgcttt tgcagggcga
     6361 agatctggat acggtgctgg ctcgtcgcgg tgcgctgccc gaaaacatcg ctttgcgtct
     6421 tgcggtacag gcggggcgcg cgctggcggc ggcccacgcg gcttcggtgg tgcaccgcga
     6481 cgtgaagccg ggcaatttgt atttggagca agggctgggc aatgaagtga tcttgcgcgt
     6541 ctgcgacttt ggaatcgcga agcgcttgct cggtggccca cagactccgc tgaccgcgac
     6601 gggtacgcag ctcggaacgc ccgactatgt tgcgccggag caactgcgtg acagcaagaa
     6661 tgtggatccg cgcgtggacg tgtggggctt gggcgcgacg ctgtacgaga tgttgagcgg
     6721 cgagccgccg tttggacatc atcagaatgt gttcgatgtg attggcgcga tcctttcgga
     6781 gccagtgccg agcttggccg agcgggcgcc ttggctgcgg gaagaggtcg tagccaccgt
     6841 tcatgaggcg ctggcgaaga agcccgagga gcgcttcggg tcgatgtcgg actttttggc
     6901 ggctctggag agtctggtgg cctcgccgtt cacggtgact cgcgctgact tggccctgcc
     6961 ttctccaaag cggcccacgg ccgccggttc caccataccc gggagcgagc catcgaagcc
     7021 gcccgacgcc gatctgcccg agctgatccc ggtggagtcg gtgcgctccg aagtcgcgcc
     7081 gatccgcgcg agtgtttccg cgaccgcgcc ctcatcactc gccccgagcg cgtccgcgac
     7141 ttcgagcgat cgctccgcga aacgaggcgg agccgcggcg aaacacggag gctcggccgc
     7201 gtccaagaac aaaggccccg aggcgccgcg ccgcgctttt tgggtcggcg tggtgctcct
     7261 gctctcgagc gcgctcgtta gttactgggt gttgcggttt ttgctatcga tgcgggcttg
     7321 agcttcggcc gcgaagtccg gcgagccggg ctgggtgccg aactgctgct cgaggactcg
     7381 gaagcgggct tcctgggcgc taccccggat gtaccggagc tggccgtcat cggcgacttc
     7441 gaggctgacg tcttgacccg catctttggc gagctcggtg agcagagcgt ccgcttcctc
     7501 cgcggagatg ccgatcgctt gggcggcctc gcgggcgtcg agcgaaccgc gatgtgcgag
     7561 gaggttgcgt gccgcatcga gccgcacggc atggctcttc tgtgcgccac tcttccgcag
     7621 cttgtgcccg ccgaacagca gcaacatccc gaagaatagg ctcgcgatcg tgataggcag
     7681 cgcaaatgcc cagccgacca aggacgcggg ccagatggag tgcagcagca ggaacactga
     7741 aacggccagt gtgagcccga ataccagcac caataccccg aagaagctgg cggctattcc
     7801 gcccactcgc gagggttgac cggcaaaggt gacgctgggt gcgacgagag gtaaccgcgc
     7861 ggcgccgcag gccacacatc gagcgccggg agccccagac tccgtgcgca gcacgatcgg
     7921 ggcgcgtctt ccgcagcttg gacatacagg gctgaagctc gacgtcgcca cagcgagcca
     7981 ctctagctca gaacccgcgc ctttggaggg ttccaatcga aaaggcacta ggagcagagc
     8041 cgaaaggcca gaacgagcag agccgaaagg ctactgcaaa accgagcgtc atggcctgat
     8101 tggccggtgc ccgagcgctg ccttctttct gagcttgatg ctactgtccg cgagccttgc
     8161 ccttgttatc catcgaggac ctgcgcgtcg agttcacgac cgaaagcgga tcggtgaccg
     8221 cgctgtgcgg ggtggatctg gcgctcgagg cggggcaatt cgcggctttg gtcggcgaat
     8281 cggggtcggg taagtcggtg acggcgctcg cggttctcgg cttgctcggg cccaatgcgc
     8341 ggcgtcgcgc gaggaagctg gagtttgacg ggcaggactt gctgaaggcg tcggcgtccg
     8401 cgctacggga gctacgcggg cggcagattg gcctcgtctt ccaagacgcc atgtctgccc
     8461 tgaacccggt ctcgagagta ggagaccagc tcacggaggt gctggtcttg catcgcggcc
     8521 tgagtcggca cgacgcgcag acggaggccg ttcgcgcgct cggtcgcgtt ggcattgccg
     8581 ggcccgagca gcgcgcgcgc caatatgctc acgagctgtc gggcgggatg aagcagcgcg
     8641 tgatgattgc gttagctctc gcagggcagc cgaagctctt gatcgcggat gaaccgacca
     8701 ccgcgctcga tgtcacgatt caggcgcaaa tcttgacgtt gttgaagacg ctgcaagccg
     8761 agctcgggct cgcggttttg ctgattaccc acgatgcggg cgtggtggcc cagtttgccc
     8821 atcgagttca cgtaatgtac gcgggccgcg tggtcgagcg ggggccgacg gccaaagttt
     8881 ggggtgcgcc cgggcacccg tatacgcgag cgttgctagc ggcgttgccg cggcccgagt
     8941 ttgttcgtcg gcacttgccg gcgattccag gtcaagctcc cgtgctgacg gagccactcc
     9001 agggctgtgc gttcagtcct cgttgcgccg aggcgttgcc gcagtgccgt cgtgaattgc
     9061 ccagaccgcg agagatcgca ccggagcacg agacgagctg ttggatcccg ccgaggcaac
     9121 ccggatgatc gatgcaccga tgaaggaggt cgcgccgttc ccgtcgcgag ccttgctcga
     9181 agtggagaac ctgagcaagg atttcccggt gcgcgatgtg ctcggcaggt cgcggggcag
     9241 cgtgcgggcg gtgagcaacg tatcgttccg cattcaacgc ggggaaaccc tagggctggt
     9301 cggcgagtct ggctgcggaa aaagcacgat tggacgggcg gtgctcggtc tgctcgagcc
     9361 cacgcatggc cacgtacggt tcgacggtgt ggatgtggcg cgcgcctcgc gcaccgagct
     9421 gattgccttg cgtcgccgca tgcagatcgt ctttcaagat ccgcacgcct cgttgaaccc
     9481 ccggctgtcg gtgtgggctg cgttggcgga gccgctcgag gtgcatggcg taacgcgcgg
     9541 agcggaaact tcccagcggg tcacgcgctt gttggaccgt gtgggcttgc ccgatagcgt
     9601 aaaaaagcgc tttccgcacg agctctcggg cgggcaatgt caacgcgttt cgattgctcg
     9661 cgcgctctcg ctcggccccg agctgatcgt ctgtgacgag gcgacctccg cgctcgacat
     9721 gtccgtccaa gcgcaaatcc tcaacctgct cctggacctg caacgcgagc ttcaggtagc
     9781 ttacttgttc attacccaca acttggcggt ggttcggcac ctcgctgatg aagtgctcgt
     9841 gatgtacttg ggaaaggtcg tagaacaggc ctctgccgcg cagctcttcg ccgcgccgat
     9901 gcacccctac acgcgagcct tgctcgcggc caatccgagt tcggacccca aagtgtcatt
     9961 tcagcccgaa cctctgactg gtgaactccc ttcgccgctc gacccgcctg cgggatgtgc
    10021 atttcatcca cgctgccgcc acgcattcga actttgtcga acccgcgtgc cggcgctgcg
    10081 agcgccagat cctcgcccac tcgcggaagc aaagaatgcg ggcacgcaga ccgacacggc
    10141 cgccgtgcag accaacgtga cggggcggac tagccgcgtc gcttgccacc tttacgacga
    10201 tgctccgtag ctggctctac ttcattggat tcttggcgct cgggctcctg ctcgtcgggc
    10261 tcaccttctc ggccgcttcg ctggaacgag ccgatttcgc gttcgtcaac ctgaccgagc
    10321 ccaagaccct cgaccccggc gccatgacgg gcgaacccga aggcaggctc gcagatgctc
    10381 tgttcgaggg cttgacccgg ctcgacgggc aaacgctcga atcggcaccg ggtgtcgccg
    10441 agagctggaa aatctcggcg gacggcacgc gctacgagtt cacgctgcgc ccgaacgcgc
    10501 gctggagtga cggtcgtccg ctcactgccc acgacgtcac gtttgcctgg cgccgcctgg
    10561 tgaacccgcg cttcggcagt gagtacgcct atctgctgaa catggtgaag tttgcggagg
    10621 cgttcaattc cttcgaggcc cagtcgaagg cgctggagcg cgcggcggca gcgctggaag
    10681 aacgcgcaga tccgagcggg cgcctcgcgc gagcgacctt tctagagctc gccaagaagc
    10741 tgggtttgcc gccgatcttc gcgccgatgg atgaccgagc gctgtcagat tggttgcgtc
    10801 atcccgagtg gatcgaacaa ccgggtggcg tgcgcgagtt cacgaccgcg ttgcgcaacg
    10861 agagtcgtcg tcgcttggag cgctatcgag atgcagagcg gcacttcggg atcgacgcgg
    10921 gtgtgttcgc gaccgacgat caccacgtga cggtggagtt ggtggctccc actccgtatt
    10981 tccttgagct tacggctttc tatccgacgt atccgctgcc tcgtcacgcg atcgagaagt
    11041 tcggggacga gcgctggttt ctgccgggtc acatcgtctc caatggcgcc ttcgtgctcg
    11101 atcaatggcg cgtcggggac cgcattcgga tgctgcggag ccccacgtat tgggaccggg
    11161 ggcgagtgaa gctgcggtcc gtggatgcgc tgccttcgga gaacgcgacc acttccctga
    11221 acttgttctt gagcggggaa gtggactggc tgcccaccaa ttcatatcca gtggatctgt
    11281 cggagtatct cgcgcagcgc ccagactatt tccgcagctc ggccctgtcc gtctatttct
    11341 atcgcatcaa ctgcacgcga aagcctttcg atgatccgcg ggtgcgccta gccatcaact
    11401 tggccgtgga ccgggaggtg attgccaaac aagtcgtcgc ccaaggccag attcccgcgt
    11461 accgggtggt cggacccggc attcccggct atcccgaggc ggagactccg ttgcgctacg
    11521 acgttgcgca ggcccgtaag ctcctggcgg aggcggggtt cccggatggc cgcgggttcc
    11581 cgaagttcgg tgtgctctac aacaccatgg atatgcaccg caagatcgcc gaggtaattg
    11641 ccgatcagct gcgccgcaac ttgggtattc aggcgacggc ctacaaccaa gaatggcaat
    11701 cttatctgac ctcgtatcgt gccctcgatt acgacctctc gcgcgccggt tgggtgcccg
    11761 actacttgga cccgaacaca ttcttggact tgtgggtcac ggacggcggc aacaactcca
    11821 ctggcttttc ggacgccact tacgacgagt tgatccgtgc cgcgagcaac gtcgaacgct
    11881 ttctgaaggc gccggagttg cgaggcggca cattcagcca cccggagcgc atcgagcgcg
    11941 cggtgcaggc cgtgcgcgac gcggccaccc cggaccaaca cctggcccag gcggtggcgc
    12001 tgaggatgga gttgttacgt gaagcggaga cgcgcctttt cagccagggc ccaccgattc
    12061 tgcccatcta cttttacgtg aacagcggca tgatcaaccc caaggtgcgc ggcttccatt
    12121 ggcgcattcc ggacggcaag ggaggatggc gcagcaacgt gcgcgatcga caccccatct
    12181 cggagctgag cgtcgatggc cgctagcacg ttgatcacgg tggctgcgct cgcggctctg
    12241 ctcgtcggca gcgcttactt gcccaagtcg tccgtgcgtt ggctcggggc ggcggccttc
    12301 ggcacattgt tcgccgtgtt cgcgcgcttc gcgttaggct ggcccgttgc cttactcgga
    12361 ggcgcactgc tcttcggcgt tgccgcccgt tgggcgcccg agctggtttt gcgacgcatc
    12421 acgttcttgg tgccggcgct gttgttgctg gtttttgcga ccactctgtt gatgttcgcg
    12481 gcgccgggca acccatttac cggcgaacgc gcgatgagcc cgagcgtgga ggcgg
//
LOCUS       DASTKU010000825         4929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_831912, whole genome shotgun sequence.
ACCESSION   DASTKU010000825 DASTKU010000000
VERSION     DASTKU010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831912"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1867
                     /locus_tag="VFQ61_28880"
     CDS             <1..1867
                     /locus_tag="VFQ61_28880"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HET9958557.1"
                     /translation="GTPLIERLHMTASSSAKLSSSNPGPLVLVMGVTGSGKTTLGTRL
                     AHRLGVAFYDADDFHPAENRTKMAANIPLEDADRWPWLDRMADESRAWVASGGAVLAC
                     SALKQAYRERLTVHVPHAKIVYLDVDREVIARRLEMRRGKHAFIANFDRVLEGQFRDL
                     EPPSDAIRLPATRHNPDELVELTFATLLRHGVCPAGRTHFCEGAPDADLTPATCESLL
                     DSLLTQLGPLGRVLLVPPDITRLASGAGDLTAMLYRKLAGQAQVEVLPALGTHAAMTN
                     EELSEMFPGIPLERFRIHDFRKDLHCLGEVPASFVERVSEGAVSYSIPCELNAHVVRG
                     KWDRVITIGQLLPHEVTGISSHDKHLFVGLGGKTTIDRTHFLGAACNLERILGKLFTP
                     VRDVLNYMAGELGQSLPVTHLMSVRAPDEQGALHLRGLFAGEGPSCFVAGARLARRCH
                     VQLLERALAHAVVYLPEREFKSTWLGNKSVYRTRLALADGAKLTVIAPGVQRFGEDAE
                     IDRLIRRHGYRDAESIKQRLSSDPELENSLSAAAHLIHGSSDGRFRICYAPGGLSREE
                     VEGVGYEYLDLADAERAFTPERLRPGPNRLATGEEVFFIPNPALGLWATADRFSE"
     gene            2055..3014
                     /locus_tag="VFQ61_28885"
     CDS             2055..3014
                     /locus_tag="VFQ61_28885"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9958558.1"
                     /translation="MPKRLRLGMLGTGLAAKLLYLPALRALSSRIELVACANRRRSKA
                     VAYAKLAGIPRVVNDADELFALPELDAVLISLPIHLQPRYVLQALAAGKAVLSEKPIA
                     PNLREGKQLVKAAARFDKPWLVGENFAFMPQVARLQRWIQSGKLGQVRLVEAVLINAM
                     NRKNPYFNTSWRAQPEHIGGFVVDAGVHLAEVLRRCFGMPTQVQNLTASFDPAVPPID
                     TALALLKFESGAVGTFTSCFSARYEGPFLRVYGSRANAELGYHGLTWLAKPGEPKVYP
                     AERGSFEVQFAHFADVVLRGAPLAFTPDQALENLALMQAIVSP"
     gene            3044..4438
                     /locus_tag="VFQ61_28890"
     CDS             3044..4438
                     /locus_tag="VFQ61_28890"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9958559.1"
                     /translation="MSRPNAKEAAADSLPSQVDVLVLGAGLTGLSAALELQRQNLSVL
                     VVEKQSQVGGHARTDEVNGFRFDRTGHLLHLRSERWRARVQQWLGEALLRIDRRSVVF
                     SHGSYRRYPFQANTLGLPPEVAYECLMGFLEAQRSNHSSPTQNTPPRNFEEYCLRHFG
                     SGFSRHFMLPYNTKLWGIEPSALGISWCERFVPRPQLADVIAGAVGLPDPKLGYNASF
                     YYPKQGIGELPRAMYRELQRRVAVPAGADPVRLGRSPERLIPHKKEAWFGAESVKYSM
                     LISTIPLTRLAGLLDPPPIGPTRAAGKLRSTSLRYLDIALRLPERHQFHWAYVPEARF
                     PFYRVGCYSAFSSEMAPPNKSSLYVELAARDPEPLDRTLAAVLPGLAEMGLLQSREDV
                     EFASVHTLEPAYVIYDDARAGALEELEDYLVSHSILSTGRYGGWNYSSMEDALEFGVS
                     AAARAVRELRGQAP"
     gene            4435..>4929
                     /locus_tag="VFQ61_28895"
     CDS             4435..>4929
                     /locus_tag="VFQ61_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET9958560.1"
                     /translation="MTSRDPRAAPARSSIVDSAGPGASTSESPDAPYVSIVIPVYNEE
                     ALLHAAVAGLREQLAELPERYEIVLAENGSTDATLKVLAALSQRHEDVRYISLPTPNY
                     GRALREGIAFARGKIVICEEIDLCDVDFHRRALELLKVDADFVIGSKLISGARDERPW
                     LRHGA"
BASE COUNT          860 a         1572 c         1546 g          951 t
ORIGIN      
        1 tggaacgccg ctcattgaaa ggctgcacat gaccgcatcg tcgtccgcca aactttcgag
       61 ctccaacccc ggcccgctcg tgctcgtgat gggcgttacg ggctcaggga aaacgacgct
      121 tgggacgcgc ctcgcccacc gtctgggcgt cgcgttttac gacgcggacg attttcatcc
      181 cgcagagaac cgcaccaaaa tggcggccaa tattccgctg gaggacgcgg atcgttggcc
      241 atggctcgac cgcatggcgg acgaaagccg ggcctgggtc gcgagcggcg gtgccgtgct
      301 tgcttgctcg gcgttgaagc aggcctatcg cgaacgactg accgtgcacg tgccgcacgc
      361 gaagatcgtc tacctagacg tagaccggga agtcatcgca cgtcgactcg agatgaggcg
      421 cggcaagcac gccttcatcg ccaacttcga ccgcgtgctc gaggggcaat tccgtgatct
      481 cgagcccccg tcggatgcga ttcgtctgcc ggctacgcga cacaaccccg acgagctggt
      541 cgagctgacc ttcgccacgc tcttgcgtca tggcgtctgc cccgcagggc gcactcattt
      601 ctgtgaaggt gccccggatg cagacctgac ccccgccact tgcgaatccc tgctcgattc
      661 gctgctcacg caactgggcc cgctcggtcg cgtgctgctg gtcccgccgg acatcacgcg
      721 cttggcctca ggcgcgggcg atctgaccgc catgctgtac cgcaagctcg cggggcaagc
      781 tcaagtcgag gtgcttccgg cgctcggcac gcacgcggcc atgaccaacg aagagctcag
      841 cgaaatgttt ccgggtattc cgctcgagcg gtttcggatc catgacttcc gtaaagatct
      901 gcactgcctg ggcgaggtgc ccgcgagctt cgtcgagcgg gtttcggagg gtgccgtaag
      961 ctattcgatt ccttgcgagc tcaacgcgca cgtcgtgcgc ggcaagtggg accgggtgat
     1021 cacgatcggg caactgctgc ctcacgaggt cacgggcatc tcttcgcacg acaagcacct
     1081 gttcgtgggc ttgggtggca agaccaccat cgatcgcacg catttcctcg gcgcggcgtg
     1141 caacctcgaa cgtatcctgg gcaagttgtt cacgcccgtc cgagacgtgc tgaactacat
     1201 ggccggcgag ctggggcaga gcttgcccgt gacgcacctg atgagcgttc gcgcgcccga
     1261 tgaacagggc gcgctgcact tgcgaggctt gttcgctggc gaagggccga gctgtttcgt
     1321 cgccggagct cggttggccc gtagatgcca cgtgcagttg ctcgaacgag cactcgctca
     1381 tgctgtcgtg tacctgccgg agcgtgagtt caaatcgacc tggctcggca acaaatcggt
     1441 ctatcgcacg cggctcgcct tggccgacgg tgcgaagctg accgtgattg ctcccggcgt
     1501 gcagcgcttc ggggaggacg ccgagatcga tcggctgatc cgacgtcacg gttaccgcga
     1561 cgccgagagc atcaaacaac gtctgagcag cgatcccgag ctcgagaact cgctctctgc
     1621 ggcggcgcac ttgattcacg gttcgtctga tggccgcttt cggatctgct acgcaccagg
     1681 gggcttgtcc cgggaagagg tggaaggcgt cggttacgag tacctcgatc tcgccgatgc
     1741 cgagcgggca ttcacgcccg aacgcctccg tcccggcccc aatcgtttgg ccacgggcga
     1801 agaggtgttc ttcattccca atccggcgct cgggttgtgg gctaccgcgg accgcttcag
     1861 cgagtgattc tgcccaatac cagcgctatt ccggcttaag cgcgcgcgta ctgcctgggc
     1921 cagtctcgcg tggtcatgtc ttgctggcca cgctttgcca agccacggct ccccctgtca
     1981 gcttcgttga gctgggtccg gaagaaggcg cgctcacctg acgcgcgcgc ccggctcgca
     2041 ctatgctgcg gcgcatgccc aaacgacttc gcctcgggat gctcggtacc ggcctggccg
     2101 caaagttgct ctacttgcca gcgttgcgag ccttgagctc acggatcgaa ctcgtcgcgt
     2161 gtgccaaccg acgtcgctcc aaggccgtag cttacgcaaa actggctggc atcccgcgcg
     2221 tcgtgaacga cgcggacgag ctgttcgcgt tgcccgagct cgatgcggtt ttgatcagct
     2281 tgccgattca tttgcagcct cgatacgtgt tgcaggcgct ggcggccgga aaagcggtgc
     2341 tcagcgagaa gccgattgcg cccaacctca gggaaggcaa acaattggtc aaggcggcag
     2401 ctcgcttcga caagccttgg ctagtcggcg agaacttcgc tttcatgccg caagtcgcgc
     2461 gcctccagcg ctggatccag agcgggaaac tcggccaagt ccgtttggtg gaggcggtgt
     2521 tgatcaacgc catgaaccgc aagaatccct acttcaatac cagctggcgc gctcagccag
     2581 aacacatcgg cggcttcgtc gtagacgcgg gcgtgcactt ggcggaagtt ctgcggcgtt
     2641 gcttcgggat gcccactcaa gttcagaatc tgacggcgag cttcgatccg gcggtgccgc
     2701 ccatcgatac cgcgctcgca ctcttgaagt tcgagtccgg cgcggtcggc acatttacga
     2761 gctgcttcag cgcgcgatac gagggccctt tcctgcgggt ctacgggagc cgtgccaacg
     2821 ccgagctcgg gtatcacggg ttgacctggc ttgctaagcc gggggaaccc aaggtgtatc
     2881 ccgccgagcg cggaagcttc gaggtccagt tcgcgcactt cgcggacgtg gtactcaggg
     2941 gcgccccgct cgcgttcacc ccggaccaag cgctggagaa cttggcgctc atgcaggcca
     3001 tcgtcagccc ctagagtgtc cgcgataacg actcgaactc gacatgtccc gccccaacgc
     3061 gaaagaggcc gctgcagact cattgccgag tcaggttgat gtgttggtgc ttggagcggg
     3121 cctcaccggc ctgtcggcgg cactggagct gcaaaggcag aacttatccg tattggtggt
     3181 cgagaagcaa agccaggtgg gcggacatgc gcgaacggac gaagtgaatg gctttcggtt
     3241 cgaccgcacg ggccacttgc tccatcttcg ctcggagcgc tggcgagccc gcgtgcaaca
     3301 gtggctcggt gaagcactgc tccgcatcga ccgacgcagc gttgtcttct ctcacggcag
     3361 ttaccggcgc taccctttcc aagccaatac gctcgggttg ccgcccgagg tcgcgtacga
     3421 gtgcttgatg ggattcctcg aagcgcaaag gtcgaaccac tcctctccta cccaaaacac
     3481 gccacctcga aacttcgagg agtattgtct gcggcacttt gggagcggct ttagccgtca
     3541 tttcatgctg ccttacaaca ccaagctctg gggcatcgag ccgagcgctc tcgggatctc
     3601 ttggtgtgag cggttcgtgc cgcgcccgca gctcgccgat gtgatcgccg gcgcggtagg
     3661 tttgccggat ccaaaactag gttacaacgc gagcttctac taccccaaac agggcatcgg
     3721 cgagttgccc agggccatgt atcgagagct tcagcgccga gttgcagtcc cggccggcgc
     3781 cgatccagta cgcctaggac gctcacccga gcggctcatc cctcacaaga aggaggcatg
     3841 gtttggcgca gaaagcgtca agtactcgat gctgatcagc acgattcccc ttacccgtct
     3901 ggcaggcctt ctcgatcccc cgcccatcgg gccaactcgc gccgccggca agctgcgctc
     3961 cacgtcactc cgctacttgg acattgcgct tcgcctcccg gagcggcacc agttccattg
     4021 ggcctacgta cccgaagcgc gcttcccttt ctatcgcgtc ggatgctaca gcgcattctc
     4081 ttcggagatg gcgccgccca acaagagcag cttgtacgtt gagctggcag cacgcgaccc
     4141 ggagccgctc gatcgtacgc tggccgcggt tttgccgggg ctagcggaaa tggggttgct
     4201 gcaaagtcgc gaagacgtgg agtttgcgag cgtgcacaca ctcgagcccg cgtacgtgat
     4261 ctacgacgac gcgagggccg gggctctcga ggagctcgaa gactacttgg tttcccattc
     4321 catcctgtct accgggcgtt atggcggctg gaactattcc tccatggagg acgcgctcga
     4381 gttcggcgtc agcgcagccg ctcgggccgt tcgtgagttg cggggccaag ctccatgact
     4441 tctcgggatc cgcgcgcagc ccccgcccgt agttcgatcg tggacagcgc tggaccgggt
     4501 gcgtcgacat ctgaatcgcc cgacgcgccg tatgtatcga tcgtcatccc ggtgtacaac
     4561 gaggaggcgt tgctgcacgc cgcggtggca ggcttgcgcg agcagctggc ggagctcccc
     4621 gagagatatg aaatcgtgct cgcggagaac ggttcgaccg acgcgacgct gaaggtgctc
     4681 gcggctcttt cgcagcgtca tgaagacgtt cgctacatct cgttgccgac gccgaactac
     4741 gggcgggcgc tgcgcgaggg gatcgcgttc gcgcgaggca agatcgtgat ctgtgaggag
     4801 atcgatctgt gcgacgtcga ttttcaccgc cgcgcactcg agctcttgaa agtagatgcc
     4861 gacttcgtaa ttggctcgaa gttgatctcg ggggcccgcg atgagcgccc ctggctacgt
     4921 cacggcgcg
//
LOCUS       DASTKU010000826         3946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1950506, whole genome shotgun sequence.
ACCESSION   DASTKU010000826 DASTKU010000000
VERSION     DASTKU010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950506"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFQ61_28900"
     CDS             <1..224
                     /locus_tag="VFQ61_28900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958561.1"
                     /translation="SDRSLDVECTPRASAVDERALANSCRRAALAVRCTGAARQLDWR
                     TQDRAANSRGRHSNRGFTPFASSSWALFS"
     gene            complement(281..589)
                     /locus_tag="VFQ61_28905"
     CDS             complement(281..589)
                     /locus_tag="VFQ61_28905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958562.1"
                     /translation="MLLKRAQTLALWAALSACLPLGCSKRLDPSECDALLERYVALLA
                     ASDRPGTAEAEVLRLKQRTRERAQHDASFAQCPARISRAQFDCAMQAPNADRLEQCLL
                     "
     gene            complement(580..891)
                     /locus_tag="VFQ61_28910"
     CDS             complement(580..891)
                     /locus_tag="VFQ61_28910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958563.1"
                     /translation="MRSTLPMRARLFLSALLSSCVLVTLTGCGRPATQKECEEIVERI
                     TLIELKARGMAENTQEIAATKEALRKTTLRDCVGRRISDEAMACVRKAGTTQQILNEC
                     F"
     gene            1383..2138
                     /locus_tag="VFQ61_28915"
     CDS             1383..2138
                     /locus_tag="VFQ61_28915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9958564.1"
                     /translation="MTRARTAAAQAVTPELMLRKATLARTSGARAKYAQRGLEHAGGL
                     DETTRALLLRQLYLSHMEGRRFEEALAVAEQMIEADVMPDVARQDAARAQIGLGQPDK
                     AVEHLRIAGRVSPPARRAFHLWTLGSVLYLHGRPHEAVGALERALRWGTTDKPLYAAQ
                     LALARLSLGEGEGVDLGALREALADAPCGQGYGQFVLGELCFAQEDFEAARRYLSAFV
                     TRVRGGRVALAVALTGEIRRADRLLRRLRRGAR"
     gene            2192..3505
                     /locus_tag="VFQ61_28920"
     CDS             2192..3505
                     /locus_tag="VFQ61_28920"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET9958565.1"
                     /translation="MLRLALSRLALLPLSLVCLLLLEQQAFAAGPDAFTSALAKGPLY
                     AGLAALAGGFLVSLTPCVYPMIAVTVSVFGARSARGRWEGAALSSAFVAGIVAMFVPL
                     GVVAGLSGSMFGSILQSRWVLVGVSLLFVVLATSLFGAFEFALPSGVTNRLAELGGIG
                     YKGAFALGLACGLIASPCTGPVLTGILTYIAQTRSAATGALSMACFALGLGAPFFLVG
                     TFAIQLPKSGRWMVQVKSVLGIVLLVVALYFLSTAFPNLASWARSTPVFYAAAAALVV
                     VGVALGAVHRDFGEMGGGVKARKAIGIVLVSGAAFALVSGASKPANAFSWHSGGLEAA
                     RAQALREKKPLLVDFTAAWCGACKQLDRVTFSAPEVRPEMARFVAVKVDATNNDDPAV
                     ESTLARFHVLGLPTVLVFDSSGREAVRYTDFVEPSTFLSAVQRVD"
     gene            complement(3758..>3946)
                     /locus_tag="VFQ61_28925"
     CDS             complement(3758..>3946)
                     /locus_tag="VFQ61_28925"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET9958566.1"
                     /translation="QLTQQQRATNVIGAFRVRHPPPATVVLVDDVVTTGATLAACQQV
                     LHAQGSHVIAVVALAYAE"
BASE COUNT          628 a         1313 c         1292 g          713 t
ORIGIN      
        1 tgtcggatcg ctcgctcgac gtcgaatgca cgccacgcgc cagcgccgtc gatgaacgcg
       61 cgctggccaa ttcatgcagg cgcgccgcgc ttgcggttcg atgcaccggt gcggcgagac
      121 aactagattg gcgaacgcaa gaccgcgccg ccaactcgcg cggacgtcac tccaaccgtg
      181 gattcacgcc cttcgcgtcg agttcttggg cgctgttttc gtgagccgtt tgcggcgttc
      241 gcgaccacca ccaaacagga aactcacgaa caccgcctgg tcacaacaaa cactgctcga
      301 ggcgatccgc gttcggagcc tgcatggcgc aatcgaactg cgcgcgcgaa atccgcgcgg
      361 ggcactgcgc gaaactagca tcgtgctgcg cgcgctcccg cgttcgttgc ttcaatctca
      421 gtacctctgc ctccgcggtg ccggggcggt cagaggccgc gagcaacgcc acatagcgct
      481 ccaggagtgc gtcgcactcg ctcgggtcca agcgcttgct gcaacccaac ggcaagcagg
      541 ccgagagcgc ggcccagagc gccaacgttt gcgcgcgctt cagaagcatt cgttcagaat
      601 ctgctgggta gtaccggcct tgcgcacgca cgccatggcc tcgtcactga tgcggcgccc
      661 gacgcaatcc cgcagagtcg tcttccggag cgcctctttg gtggccgcga tctcttgcgt
      721 attctcggcc atgccccgcg ccttgagctc gatcagcgta atccgctcca cgatctcctc
      781 gcactctttc tgcgtggcgg ggcgtccgca gcccgtcagc gtaacgagta cacacgagct
      841 gagcagtgca gagagaaaca accgggcacg catcggcaaa gtggatcgca tgatttcggg
      901 gcagcgagat agtttcggcc gatggacacg tacggcgccg gtccacgacg gcgcggacaa
      961 tatggagccg gcgcacgaat tcagccagac ccaatcttcg cggcagcggg ctccccgccc
     1021 cagtccgaag gcctccccgc ccgcgaggtc acccacgaaa ggagcgcctt cccggaaggc
     1081 cccccagaaa ccccctaacc aagagacgac cccgcgcccc tcagcactag ccaaaaaaag
     1141 ccagcactag ccaaaccagc cgaaagcagg aatgccgaag caggaatgcc gaagcaggaa
     1201 tggcgaagca gcaatccaga gccctccctc cagcacccac caaaactcga gcattccgtc
     1261 ggaagcacat gaaagaaaag aagcacatga aagaaaacaa gccgtctggg tagctcgggc
     1321 cgtcgggtcg gatttgggcc gaaatctgga cgtgtgtaca ggccttgcta atctcacagg
     1381 tgatgacgcg cgctcgaaca gcggccgcgc aggccgttac gccagagctc atgttgcgga
     1441 aagcaacctt ggcgcgcacc tccggtgccc gggcgaagta cgcgcaacgt ggcctggagc
     1501 acgccggtgg cttggacgaa accacgcgcg ctcttttgct ccggcagctc tatctctcgc
     1561 acatggaggg gcggcgtttc gaagaggcgt tggccgttgc cgagcagatg atcgaggccg
     1621 acgtcatgcc ggacgtagcc cgtcaagacg cggcgcgggc gcaaatcggg ctgggtcagc
     1681 ccgacaaggc cgtagagcat ctgcgcattg ccggtcgcgt gtcccccccg gcgcgccgcg
     1741 cgttccacct ttggactttg ggtagcgtgc tctacctgca tggtcgcccc cacgaggcgg
     1801 tgggggctct cgagcgcgcc ctgcgctggg gaaccacaga taaacccttg tacgccgctc
     1861 agctcgcctt ggcgcgcctg tccttgggag aaggcgaggg cgtggacttg ggtgctttgc
     1921 gggaggcctt ggccgacgcc ccctgcggtc aaggttatgg gcagttcgtg ctcggcgagc
     1981 tgtgcttcgc gcaggaagat ttcgaagccg cgcgccgcta tttgagcgcg ttcgtcacac
     2041 gagtgcgcgg ggggcgcgtg gccttggccg tggcgctcac gggagaaatc cgccgcgcgg
     2101 accggctctt gcggcgtctc cggcgcggcg cgcgctgaca tgcaggtccg cggctttcgc
     2161 ccacggattc tagcgctaaa ctagccgccc tatgctgcgc ctcgccctgt cgcgcctcgc
     2221 cctgctgccg ctcagcctcg tttgcctgct cctactcgag caacaggcct tcgccgcggg
     2281 cccggacgcc tttacttccg cgctggccaa gggcccactc tatgcgggtc tggccgctct
     2341 cgcgggcggc tttctcgtca gcctcacccc ctgcgtctac ccgatgattg cggtgacggt
     2401 cagcgtgttc ggagcgcgta gcgcgcgggg acgttgggag ggcgccgcgc tttcttctgc
     2461 gttcgtggcc ggcattgtcg cgatgttcgt gccgctcggg gtggtcgcgg gcttgtccgg
     2521 ctccatgttc ggctcgatct tgcagagccg ttgggtgctc gtcggggtct ctctgctgtt
     2581 cgtcgtgctg gccacctcgt tgttcggcgc cttcgagttt gccctacctt cgggcgtgac
     2641 caatcgcctc gctgagctcg gcggcattgg ctacaaaggc gcgtttgcgc tgggcctggc
     2701 ctgcggcttg attgcgtccc cgtgcacggg ccccgtgctc accggcattc tcacgtacat
     2761 cgcgcagacg cgcagcgccg cgaccggtgc gctctcgatg gcatgtttcg cgctcggctt
     2821 gggtgccccc ttcttcctcg tcggtacctt cgcgatccag ctgcccaaga gcggccgttg
     2881 gatggtgcag gtcaaatcgg tgctcgggat tgtgctcttg gtggttgcgc tctacttcct
     2941 cagcaccgca ttccccaacc tggccagctg ggcccgctcc actcccgtct tctacgccgc
     3001 cgccgcggcg ttggtcgtgg tcggggttgc gctcggcgcc gtgcaccgag acttcggtga
     3061 aatgggcgga ggcgtgaagg ctcgcaaagc gatcggcatc gtgctcgtct cgggcgccgc
     3121 gttcgccttg gtgagcggtg cgagcaaacc tgcgaacgca ttcagttggc acagcggcgg
     3181 gctcgaagcc gcacgcgctc aggcgctgcg cgagaagaag ccgctgctcg tcgacttcac
     3241 ggcggcttgg tgcggcgcct gcaagcagct cgaccgcgtt accttctccg cgcccgaggt
     3301 gcgccccgag atggcacgct tcgtcgcggt caaagtggac gcgaccaaca acgatgatcc
     3361 cgccgtcgag tccacgctcg cccgcttcca cgtgctcggg ttgcccacgg tgctggtgtt
     3421 cgactcttcg gggcgcgaag ccgtgcgtta taccgatttc gtcgagccga gcaccttctt
     3481 gtccgcggtt cagcgcgtgg attgagcttt ctcttggcgg ctttcatggc cgctcgcgag
     3541 cacgggcgcg aagcgcgacg tgttcccgcg cgagcgcaga ctcggggccc gatcgggccc
     3601 gtgaaaccgc gcaagcggcg ctcatcgacg agcgttattg ggggcggcgg gcgcggtgtt
     3661 ggggcgcacg acggggaagt acggctgcgc cggggtgaac tcggaccgcg cggcgcgcac
     3721 gttacttcga ggccgaaaaa aaccaaggct gcgctcacta ctcggcatag gccagcgcga
     3781 cgacggcaat tacgtggctg ccttgcgcgt ggagtacctg ctgacacgcg gcgagggtgg
     3841 cgccggtggt gaccacgtca tcgaccagta ccaccgtggc gggcggcggg tggcgtacgc
     3901 gaaaggctcc gatcacgttg gtggcgcgtt gttgctgagt cagttg
//
LOCUS       DASTKU010000827        10331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_832037, whole genome shotgun sequence.
ACCESSION   DASTKU010000827 DASTKU010000000
VERSION     DASTKU010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832037"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFQ61_28930"
     CDS             complement(<1..189)
                     /locus_tag="VFQ61_28930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958567.1"
                     /translation="MSRKTKREKQRARRALDYRATMSLHGDALQVAPYVDQAAAIEKR
                     VATPTQGANGEVTVYDVTC"
     gene            complement(186..458)
                     /locus_tag="VFQ61_28935"
     CDS             complement(186..458)
                     /locus_tag="VFQ61_28935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958568.1"
                     /translation="MATNGEIDLTLQIKDNTQPLTVRVPATLTPNEIIQDLQAQNQIP
                     RPANATVTMTHNKTNLQPDIPLSRQGVADKDALGVVWNGELAGKAR"
     gene            complement(502..1677)
                     /locus_tag="VFQ61_28940"
     CDS             complement(502..1677)
                     /locus_tag="VFQ61_28940"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET9958569.1"
                     /translation="MASPAATYDCLRAAGRDPEILHRRRLGVVGCGNAGSNTTSTLSV
                     LGIETVYIDMDVVEPKNLAHSPFLDVVATPNGSPRFKAELLASAHVEVSRRADQVAQW
                     AALPIQAVPWGVLRKLHGLIVGVDDGPSRGWIARTAALLGIPTVVVGFLPPTGNFVAV
                     SNRDESAPCFFCLRPRESPLRASCSLYSSPSDGVNPALQTAAAATMNVAIEAMTRFWH
                     EDYRYDGKVFRLDLDDGASDLTGFRVHPDCPGPHERLPEPSPAPFEADSPAAAVLELA
                     EREGLRRPALELPSRYVVSLPCRACGRVVPVGKPEWMVRTAPVCPSGCAVGGAVVAPV
                     FCEVVAEGTALAARSLSSLGVGPLGLCVVADAEGDEVRVYELPGHEHSVLNTCVRVR"
     gene            complement(2149..2673)
                     /locus_tag="VFQ61_28945"
     CDS             complement(2149..2673)
                     /locus_tag="VFQ61_28945"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET9958570.1"
                     /translation="MATNEDASTETLETFRTEAGARRFFERAVPALTREAPTWCPELA
                     WDTSVDAYLAVATHPERFEAPAQAYVYALTKIRRDRWRRRRRTWDREVTLDDVRAPVD
                     PRTALETLAVSEELANLLGRLPQKLRETFVAIEGLGASAAEFAVEEGIAIQTVYNRMR
                     HARRLLKRFIEEGA"
     gene            2758..3603
                     /locus_tag="VFQ61_28950"
     CDS             2758..3603
                     /locus_tag="VFQ61_28950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958571.1"
                     /translation="MAYWGLDHIRAKSADVRAAFGDVLTRRQVARRPNGKQWKFLQAC
                     LETLLGERTPALSISKSQATQYKFEIEDRLRRFYRSPGKPVSLVFALEHQDHAIATEA
                     VTESYPTTMGYVLLVSDARPENVPRLPGSELQEFLLRVVDDAARAELAAYRSLPTMDL
                     SVLNSALVVGSPAFLRIRNIVELHDKKGWCISAPPSNPSSMRMLKSRVVEAGPDRAIV
                     NTEEYWYLRWWSLAKREYAYVYQETNRQTYFLRRDGERWLVEENKYTQPRRTTPQRSG
                     PKRAG"
     gene            3793..4395
                     /locus_tag="VFQ61_28955"
     CDS             3793..4395
                     /locus_tag="VFQ61_28955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958572.1"
                     /translation="MGELLNTELQTSPPDSSPVKLIIEGIDRLGKDTLIRGIQDRRGY
                     HLVLHYTKPIPLAFYEGSGHPAGRQYQEASFRTMFQMLRVAETQLICNRSHLGECVYA
                     PLYRHYSGDYVFQLEQLFEVAAIPRIRLVLLTEDFSVSTHFRDDGLSLGAAGQRQKEQ
                     ALFLEAFGRSTFADKRVVCVTDTTTGGFRSATEIVTEALK"
     gene            4416..5396
                     /locus_tag="VFQ61_28960"
     CDS             4416..5396
                     /locus_tag="VFQ61_28960"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: protein motif:HMM:NF012524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET9958573.1"
                     /translation="MDDLLLGVFRRMLRVKDKISPSRGEAREEFGALLELTNPRARLS
                     RTESRQQTLFSCLGELLWYLAKSNELDFINYYVPHYRKESTDDETVRSAYGPRLFNNH
                     GINQIENALTLLRAGHDSRRAAVQLFEAYDTDPRNHESPCTCTLQFVLRKKRLHLLTF
                     MRSNDVFLGLPHDVFAFTMLQEIVASHLSVEPGSYKHAVGSLHLYDEHRKLAKEYIAE
                     KYQPTTDAMPPMPLGDPWPAIRLVLRAERDIRGGQTVNPSQLQLDPYWQDLVRLLQIF
                     GHFSKNKKDQIPPLQKSMHSPIYDTYIQKKLESSPKAREPIAHKQLRLFD"
     gene            complement(7157..7744)
                     /locus_tag="VFQ61_28965"
     CDS             complement(7157..7744)
                     /locus_tag="VFQ61_28965"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HET9958574.1"
                     /translation="MRQGEILANGVLCKPWDPERFQEQIPRIRDLCNKKIPSIFPTLQ
                     SLCSDCGVAAIVLQAPSGCRASGATRFLSPSKALLLLSFRYLSDDHFWFSFFHEAGHL
                     LLHGERFVSVEGMDRSTSSQEHEANQFAANVLIPPEERQRMLALRSEFREIVRFALRI
                     GVSPGVVVGQLQHAGRLGPHQQNRLKRRFSWEREE"
     gene            8332..8472
                     /locus_tag="VFQ61_28970"
     CDS             8332..8472
                     /locus_tag="VFQ61_28970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958575.1"
                     /translation="MTNTHDQLKPVAKLKGYFFSSWNRVRPARDDILYVRSGTEVGEP
                     MA"
     gene            complement(8682..9071)
                     /locus_tag="VFQ61_28975"
     CDS             complement(8682..9071)
                     /locus_tag="VFQ61_28975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958576.1"
                     /translation="MYDFRNPGVGEEGFLWSQVASSWQGWTVEEFRSALKHGLARHAF
                     ERDQRALEWCDCCVLVLPSGMSAHLEAGWCAGRGKPVLVYAPEIREAELMYKLFDSDG
                     VTPLFTTIGDVLSAVAMARSAEALARS"
     gene            complement(9388..>10331)
                     /locus_tag="VFQ61_28980"
     CDS             complement(9388..>10331)
                     /locus_tag="VFQ61_28980"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9958577.1"
                     /translation="STGRSPATVRRYGDVLHDLDRHSAGAEPSAIPRGDLIRFAAAPR
                     TDGGHRSPAGVNLRIAVLKAAFAYLLNEGLLPDNRAATLVAVKQPRRTPDYLSTNEVA
                     RLLTYLATRSSSRRLRDLVMTLVFWQTGLRVAEVARLTWGDLDLERHCLRDVLVKGGH
                     RRDVPLNDEVVAVLSGYRVTSANPSFERPIFTGFQGRSLSVRAIQALFATWRAELGWA
                     RRLHPHVLRRTHATGALALGVDIATVADLLGHSDLRAVMAYAAVQDGPRRAALRRLGD
                     LVPAEILKQIRGVASAANDAEETTCVEERFDESGAAA"
BASE COUNT         2028 a         3269 c         3140 g         1894 t
ORIGIN      
        1 gcacgtcacg tcgtacacgg tgacctcgcc gtttgcgccc tgggtgggcg tcgcgacacg
       61 cttctcgatc gcggccgcct ggtccacata gggagcgact tgaagcgcgt cgccgtgcaa
      121 gctcatcgtc gctcggtagt cgagcgcgcg gcgggcgcgt tgcttctcgc gcttggtctt
      181 tcggctcatc gcgctttccc cgcgagctcg ccgttccaaa cgactccgag cgcgtccttg
      241 tcggccaccc cctgccgcga gagcgggatg tccggttgca ggttggtctt gttgtgcgtc
      301 atcgtcaccg tcgcgttcgc gggccgcggg atctggttct gcgcctgcag atcctgaatg
      361 atctcgttcg gcgtgagcgt cgccggaacg cgaacggtga ggggttgcgt gttgtccttg
      421 atttgaagcg tcagatcgat ttcgccgttg gttgccatgc tggcctcctg cttccccaag
      481 aaggcagcgc gcagcgcaga cctaccgaac gcggacgcac gtgttcaaca cgctgtgctc
      541 atggcccggc agctcgtaga cgcgaacctc gtcaccctcc gcatccgcaa caacgcaaag
      601 cccaagcggc ccgacgccga gtgaagaaag cgagcgcgcc gcgagcgcag tcccctccgc
      661 gacgacttcg cagaacacag gcgcgaccac cgctccaccg accgcacacc cgctggggca
      721 gacgggcgca gttcgcacca tccactcggg tttgcccacc gggaccacgc gcccgcatgc
      781 tcgacacggc agagagacga cgtatcgcga cggcagctca agtgcgggac ggcggaggcc
      841 ctcgcgctct gcaagctcga gaaccgcagc cgcgggcgag tccgcctcaa agggggccgg
      901 cgacggctca gggagccgct cgtgggggcc gggacagtcg ggatgaaccc gaaaccccgt
      961 gaggtcgctc gcgccatcgt cgagatcgag ccgaaacacc ttgccgtcgt agcggtagtc
     1021 ctcgtgccag aacctcgtca tcgcctcgat ggcaacgttc atcgtcgccg cggccgcggt
     1081 ctggagcgcc gggttcacgc cgtcgctcgg gcttgagtag agcgaacaag aggcgcgcag
     1141 cggcgactcg cgcggacgga ggcagaagaa gcacggcgca ctctcgtcgc gattcgagac
     1201 ggcgacgaag ttgcccgtcg gcggcagaaa gcccacgacg accgtgggga taccgagcag
     1261 cgccgccgtt cgcgctatcc acccacgaga cgggccgtcg tcaacgccga cgataagacc
     1321 gtgaagtttc ctcagaacac cccacggaac agcctggatc ggcaacgccg cccattgcgc
     1381 gacttgatct gctcttcgag acacctcgac gtgcgcgcta gccaagagct ccgccttgaa
     1441 ccgcggcgag ccattgggtg tcgccacaac gtcaaggaat ggtgaatgcg cgaggttctt
     1501 cggctcgacg acgtccatgt cgatgtacac cgtctcgata cccaacaccg agagcgtgct
     1561 cgtcgtgttc gagccggcgt tgccacagcc gacgacgccg agacgtcgtc gatggaggat
     1621 ctccggatcg cgacctgcgg cacgtagaca gtcgtaggtt gctgcggggc tagccatcga
     1681 gcactcctcg ctggcagctt gcgtggtcac cctcgtcgag gcgcaaccca cacaagcggc
     1741 acactcggcc acgaactgtt ctgttagtgg gcgcgctgag ccgcacgcgg gtgtacggca
     1801 tgacgacaac gcgcggacgc tgcggaggcg cggctcgcgc gggcgtgagc gctggcaaga
     1861 gtggtcgtcc ggccgcgaga gaagcggtgg gcgctggcgc actgcgcgcg cttggcgtgc
     1921 cgagcgatcc acgcaccacc gagcccagct ggctcggcgc ttgcggcacg agccacggcg
     1981 cggagtcagg cgtactcact tgcccgcttc ggcgtcgccc aaaaccgagc aggagcatga
     2041 caatgatgcc gagaacgaac ccgacgacgc ccgacacgac ggcgtcactt gaatcgggcg
     2101 tcgagcacgc aggtgacgtg actccgatct cagcgagccc agcggacact acgcaccctc
     2161 ctcgatgaaa cgcttgagca gccgtctcgc gtgccgcatg cggttgtaga cggtctggat
     2221 cgcgatgcct tcttccaccg cgaactcggc cgcggaagcg ccaaggccct cgatcgcaac
     2281 gaacgtctct cggagcttct gcggtaagcg acccagaagg ttcgcgagct cctctgacac
     2341 ggccagcgtc tcgagcgcgg tccgtggatc aacgggcgcc cgcacatcgt cgagcgtgac
     2401 ctcccggtcc caggtccgtc gccgccgccg ccaccggtcg cgtcgaatct tcgtgagcgc
     2461 gtacacgtag gcttgagcgg gcgcctcgaa gcgctccggg tgcgtcgcaa cggcgaggta
     2521 cgcgtccacc gacgtgtccc acgcgagctc cgggcaccat gtcggcgctt cacgggtcag
     2581 cgctgggacc gccctctcga aaaatcgccg ggcgccagcc tcggttcgga acgtttcgag
     2641 ggtctcggtg gaagcgtctt cgttcgttgc catcacctcc tatagacgcc tcacccttgc
     2701 taactactgc aatctccaat agttgtattc caattggagt tttgattgga gtaacggatg
     2761 gcttactggg gcctggatca catccgcgcg aagtcggccg acgtgcgcgc cgcgttcggc
     2821 gacgtgctca cgcgccgcca ggtcgcccgc cgtccgaacg gtaagcagtg gaaattcctc
     2881 caggcctgcc tcgagactct cctcggcgag cgcacccccg cgctcagcat ctcgaagtca
     2941 caggcaactc aatacaagtt cgagatcgag gatcggctcc gccgtttcta tcggtcccct
     3001 ggcaagccag tgtcgctcgt cttcgcactc gagcaccagg atcacgcgat cgcgacggaa
     3061 gcggtgaccg agtcgtatcc gacaacgatg ggctacgtgc ttctcgtttc cgatgcgcgc
     3121 cccgagaatg tcccacgtct cccaggcagc gaactccaag aatttctcct ccgtgtcgtt
     3181 gacgacgccg ctcgcgccga gctcgctgcg taccgcagtc tccccaccat ggacctctcg
     3241 gtgctcaact cggcgttagt cgtcggatcg cccgccttcc tgcgaattcg gaacatcgtc
     3301 gagctccacg acaagaaagg ctggtgcatc agcgcaccgc cctcgaaccc ctcctctatg
     3361 cgcatgctga agtcgcgcgt ggtcgaagcc gggccggacc gtgccatcgt caacactgag
     3421 gaatactggt acctccgctg gtggtccctc gcgaagcgcg aatacgccta cgtctatcag
     3481 gagaccaacc ggcagacgta cttcctccgg cgtgacggtg agcgatggct ggtcgaggag
     3541 aacaaataca cgcagccgcg caggaccacc ccccaaagga gcgggccgaa acgcgccggt
     3601 tgaccctgca gtggtgaacg gctgaacggc cattcagcgc gatcgaagct gaacccgcag
     3661 agccgggcga tgccccacca ccgaccgggc cctccttgct tgcacactcc taggcacgct
     3721 cgatattctg tcaggtggtt cagccttcgt ccgtcgcacg tcagtgtcca aacctagcaa
     3781 tgtggaggct ccgtggggga actgctcaac accgagttgc aaacgtcgcc gcctgactcg
     3841 agccccgtca agctgatcat cgaagggatc gaccgcctgg gtaaggacac gcttattcgg
     3901 gggattcaag accgccgcgg ataccatctg gtccttcact acaccaaacc gatcccgctg
     3961 gcattctacg agggatcagg acatccggcg ggccgccaat atcaagaagc gagcttccgc
     4021 acgatgtttc aaatgcttcg cgtcgcagaa actcagctca tctgcaaccg gtcacacctt
     4081 ggcgagtgtg tgtacgcacc gttgtaccga cactactccg gtgactatgt cttccagctc
     4141 gagcagctct tcgaggtcgc ggcgatcccg cgcattcgat tggtgctcct cacagaggac
     4201 tttagcgtca gcacgcactt ccgcgacgat gggctgagtc ttggagccgc agggcagcga
     4261 cagaaggagc aggccctttt tcttgaagcc ttcggtcgct cgacattcgc cgacaagagg
     4321 gtggtgtgcg tcaccgatac cacaactggg ggtttccgtt ctgcgacgga aatcgtcacg
     4381 gaagcactga agtaatgcac ctagtagcag acaccttgga cgacctcctg ctcggtgtct
     4441 tcaggcgaat gctgcgggtc aaggataaga tcagccccag ccgcggcgaa gcgcgcgagg
     4501 agttcggcgc gctccttgag ctaacgaatc ccagagcacg gctgagcaga accgaaagca
     4561 ggcaacaaac gctgtttagc tgcctcggcg aacttctctg gtatctcgca aagtccaatg
     4621 agctggactt catcaactac tacgtcccgc actacagaaa agaatctacc gacgacgaga
     4681 ccgtccgaag cgcgtacgga ccgcgcctct tcaacaacca cgggataaac caaatcgaaa
     4741 atgcgctcac gctgctccgc gcaggacacg actcgcgacg ggcagcagtc caactctttg
     4801 aggcgtacga cacggacccc cgtaaccatg aatcgccctg cacctgtaca ctccaattcg
     4861 tacttcgcaa aaaacgcctg cacttactga cgttcatgcg ctcgaacgat gtcttcctcg
     4921 ggcttccaca tgatgtcttc gcgttcacga tgctgcagga aatcgtcgca agccacctga
     4981 gcgtagagcc tggctcctac aagcatgcgg tcggcagcct tcatctgtat gacgagcata
     5041 gaaagttagc caaagagtac atcgcagaga agtatcagcc gacaacagat gcaatgccgc
     5101 cgatgccact gggcgatcca tggccggcga ttcgactcgt actacgggca gagcgagaca
     5161 tccgaggcgg gcaaaccgtc aatccatctc aattgcaact tgacccatac tggcaggacc
     5221 tagtacggtt gcttcaaatc tttgggcatt tctccaagaa taagaaagac caaatcccgc
     5281 cgctacaaaa gtccatgcac tcaccaattt acgacaccta catccagaag aagttagaga
     5341 gctcgccgaa ggcccgcgaa cctattgctc acaagcagtt acgactgttc gactagagga
     5401 ccagacgtgt taggcggaac cgtatctatg ctaattcagc gtctggcagg tggcgcgctt
     5461 tcgcatacga gcgtcattcc ttggagttgc cctgtacctg cctttgggga cgtccaggga
     5521 gcctccgtag cgaccctggg gctgaacccg agcaatcggg agtttgtgga cgagacgggg
     5581 gaagaactca gtggcaacca gcggcgcttt cctacgcttc gctcgcttgg attgaagcac
     5641 tggctcgatg cacggcctcg gcatgttgtc gccgttgaaa gatcgtgtcg gacctacttc
     5701 tccgtgaacc catacgacac ctggttcaga aagctcgacg cgattatcgg cagcacaggg
     5761 gcatcgtatt acggcgactc tgcaagggcg tgccacctgg atctcatccc atatgccaca
     5821 agcaacaaat ggacgaatct ctcgcatgtc gaaaggcgtg cgctcggcca agctggaggt
     5881 gatgcgctgg gaaccctgct acgcaactca tcgatccgcc ttctcgtgct aaacggtcgg
     5941 tctgtggttt ctgcttttca aagcgcggcc agcgtcgact tgaagcgacg agcgatgtcg
     6001 aactggtcat tgccgcgctc gtctggtttg ggggtgtcgg gcttcgctta ctcagggaca
     6061 attacacgct tcgcagcaag cgatcttgga cgagagatcc gtgtactggg tttcaatcat
     6121 aacattcaaa gtagcttcgg cgtaaccacc agtgtcatca cagccattag acgctggatc
     6181 gctcgagagg cctaggtggg gacggggcgg gccgctttga gtcgtcagct ggcggaaggg
     6241 ctggccgcat atgagcgcca gtctcggcca ctgcgcggca taggtcgccc gtccgcgcgt
     6301 gctgcgttcg tcggacagtt ggtggaaagc gtccggcgcg tggaatatgt cgctaccatg
     6361 agggatcgaa cactaagccc tcagcggttg gacccatcaa gtgagctgtt cgatcccttg
     6421 atggctgctg ccctgcgcgt ccagagaggc gatgttgaag aggcttcttg gctcgtattc
     6481 ctctccgtcc atttcgggaa gcatcgtcga gatggatggc ggctcgcgcg cgacgtctat
     6541 aggggaagcg gaaacgggga tcggtggacc tgggatgcca ctagtgctga tccgcgcgcg
     6601 ttcagccgtt ggctagcttc cgcggcgcga aagtggaaaa cagcgggaac gcaagctcgg
     6661 ttcggaaacc accgcaagta cgagagtctg aacggtacct cggccaacgg caccggagcg
     6721 gtcgttcaaa gctatgtgcg atggatcgga ccgcaacggt cgcatgcgca tgtaatagag
     6781 tcctgcctag acgcggtgca ccaggaccca cgactagcgt ttgaccagct ctatcgctcc
     6841 atgcgggtgg tgaggcgatt tggtcgcacg gcgcggttcg actatctcac gatgttaggc
     6901 aaacttgcca tcgccgccat tgagcccggt tccccttact tgagcggtgc gacaggtcca
     6961 ctcttcgggg cgcgtctgct attcagcgga agggctgacg caagactgcg ggcggcagag
     7021 atggaacaat ggttgaatgg actcggggca actctacgcg ttggtatgca ggtgatagaa
     7081 gacgcactgt gtaactggca aaagaatccc gaaaagtttg tgccgttcag aggatagccg
     7141 acatcagcct agggtcctat tcctcgcgct cccaggagaa gcgccgtttc aatctgtttt
     7201 gctggtgagg ccccaaccgt ccagcatgct gtagctggcc gaccacgacg ccgggcgaaa
     7261 caccgatccg cagtgcgaac ctgacgatct cgcgaaactc ggagcggagg gcgagcattc
     7321 gctgcctttc ctccggaggg atgagcacgt tagccgcgaa ttgattcgct tcatgctctt
     7381 ggctgctcgt tgaacggtcc atgccttcga ccgacacgaa ccgctcgccg tgcaacagca
     7441 ggtgtcccgc ctcgtgaaag aagctaaacc agaagtggtc gtccgaaagg taccgaaaac
     7501 tcaaaagcag gagtgccttt gatggtgaga ggaaccgcgt cgcaccgctc gctctgcagc
     7561 cggagggcgc ctgcaggacg atggcggcaa cgccgcagtc cgagcagagg ctctgcagag
     7621 tggggaaaat tgaggggatc ttcttgttgc agaggtccct tatcctcggg atctgctctt
     7681 gaaaccgctc gggatcccag ggcttgcata gtaccccgtt ggcaagaatt tcgccttgcc
     7741 tcaaccacgc ggagaccgcg ccctcttgcg acctgaacga aggagaagtt cgaaaggcag
     7801 ccgagtccag cgttccccgg gaccatgcgc gccactcctg aacggtgctc gcattgaaaa
     7861 atctgagaca ttcttgggcc tgcgcgggag ccgaagcggc cttcgctagc cacccgaact
     7921 tcaccatatc agctagcgga agcgtcctga gccattcggt ttcaccgggc gctcgggtag
     7981 gagtgagatt aagtcgttca agatccttcc aatagtgact atcgcgcgcc atccaaaacg
     8041 aggcagaggg gcctaacaca gcgtgcagcc gcgacgcgat tgctggcgtt attgccgtta
     8101 ggccctcaat tagatcgtcg gcttctactt gcgactgacc cattcggtgg gcgaactcca
     8161 gaagactcag ctcctgttcg ttcaacagat ccaggattgt gtcgcctgga ggagacgccc
     8221 aatcgggggc gaacgcttcg ctctcgttca tggccgaccg atcctcacta ttcggactcg
     8281 cgttactcgc ccccagtcga cagcgccgcc ggatgtgcgg ggcactttct tgtgaccaac
     8341 acacacgatc agctcaaacc cgtcgccaag ctcaagggct acttcttcag ttcctggaac
     8401 cgtgtgcggc ctgcccgcga cgacatcctc tatgtgcgca gcggcacgga ggtcggcgag
     8461 ccgatggcgt aggttctcgc tggcctcttg cccgagctcg tcagccgcct tcgtgctgcc
     8521 ctcacatagg ctgcgaagac ggatggtgtc gaacgcgaag tccaccgagt ccgacgctac
     8581 cataacacgg gacgttccaa gtggctcgag ccggcccaac aatggcagcc ggctgcaagc
     8641 cacattgact tgctcctcct cggattgcac gacgagcgcc attaggaccg agcgagggct
     8701 tcggcggagc gggccatcgc gactgcggac aacacgtcac ctatggtcgt aaacagcggc
     8761 gtcacgccgt cggagtcgaa tagtttgtac atgagctccg cttctcgaat ttcgggagca
     8821 tacacgagca cgggtttccc cctcccggcg caccatcccg cctctagatg ggcgctcata
     8881 cccgacggca gcaccagcac gcagcagtcg caccattcga gcgccctctg gtcgcgctcg
     8941 aacgcgtgac gagcaagccc atgcttcaac gcgctgcgaa attcctcaac ggtccacccc
     9001 tgccagctcg acgcgacttg ggaccacaga aacccctcct cgcctacgcc cgggttgcga
     9061 aagtcgtaca ctaaatgtcc agcatcacgc agggaacgaa caacctcggg ttgatggagg
     9121 ttcctccacg aggacgcgac gtatatcttc atctcgccaa cccagagctg atctttggac
     9181 ctgctgagct acagcttcca ctgtcatgga ggcgcgggtg cgccaagcgc gctgcgagtg
     9241 cacccgattc ggctgtggat agtctttagc acgggctgct cgtttgtgca gtgcccgatc
     9301 tcggcgcgcc tgccgcggcg gacattaatt ggccgacccg cgggggcaag ctcgtccgct
     9361 cgcgtcacga cgcgggggcg tctgtagtta ggcagcggcc cctgattcgt cgaaccgctc
     9421 ttctacgcaa gtggtttctt cggcgtcgtt tgctgcggat gccactcccc ggatctgctt
     9481 caggatttcc gctggaacca aatcgccgag ccgccgcagc gccgcccggc ggggaccgtc
     9541 ctgaacggcg gcgtacgcca tgacggctcg caggtcgctg tggccgagaa ggtcggcgac
     9601 ggttgcaatg tcgaccccga gcgcgagggc gccggtggca tgcgtgcgcc ggaggacgtg
     9661 aggatggagc cgccgcgccc agccgagttc agcacgccag gtcgcgaaga gggcctgaat
     9721 ggcacggacg gacaggctgc gaccttggaa ccccgtgaag attgggcgct cgaaggacgg
     9781 gttcgcactc gtgacgcgat agccgctgag caccgcaaca acctcgtcat tcagcggcac
     9841 gtcgcgtcga tgtccgccct tcacaagcac gtcgcgcagg caatgccgtt caaggtcgag
     9901 atctccccag gtcaggcggg caacctccgc gacacggagg ccggtctgcc agaagacgag
     9961 cgtcatgacg aggtcgcgaa ggcgccgcga actcgagcgc gttgcgaggt aggtcagtag
    10021 gcgggcaact tcgtttgtcg agaggtagtc cggcgttcgg cgtggttgct tcaccgcgac
    10081 gagtgtcgcg gcgcggttgt cggggagcag tccctcgttg agcaagtacg cgaatgctgc
    10141 cttcagcacg gcgatgcgaa ggttcacccc ggcgggcgag cggtggcccc cgtcggtgcg
    10201 gggagccgca gcgaaccgga tgaggtcgcc acgcgggatc gcacttggct ccgcacccgc
    10261 gctgtgacgg tcgaggtcgt gcaatacgtc accgtaccgg cggacggttg ctggcgatct
    10321 tcccgttgac t
//
LOCUS       DASTKU010000828        12211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_163149, whole genome shotgun sequence.
ACCESSION   DASTKU010000828 DASTKU010000000
VERSION     DASTKU010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12211
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163149"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /gene="cobJ"
                     /locus_tag="VFQ61_28985"
     CDS             <1..863
                     /gene="cobJ"
                     /locus_tag="VFQ61_28985"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: protein motif:HMM:TIGR01466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HET9958578.1"
                     /translation="SEHVTDQLSIQRGSLSIVGIGPGAHAHTTPRALEAIARADVVVG
                     YVTYIRLVADLVQGKEIVRTGMTEEIERARVAVERARAGAQVAIISSGDAGVYGMAGL
                     VFQVLKESGWRRGDAPELQLIPGITALNACASLVGAPLGHDFCAISLSDLLTPWAVIE
                     RRIRAAAESDFVIGLYNPASGRRTRQIVRAHEIISEHRAPDTPVALVKSAYRRREERV
                     LTDLAHCLDYEIGMLTTVIVGSTQTFEFEGYMVTPRGYANKYTWDGAPRPGQTPGRSL
                     IVASSPGEEP"
     gene            860..1324
                     /locus_tag="VFQ61_28990"
     CDS             860..1324
                     /locus_tag="VFQ61_28990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958579.1"
                     /translation="MSAWLGRISGALLLQRPTPLPEGAAGFEYYLIGDTKRPCNWEAL
                     GFVAPPQRDPLREPWLKLVPLSFQGLSGKLPFQGPVLRLPHADQAAVELLARRLLVPR
                     NGSVSERLWRLVLGAEDDEDVLESPSELDAGWLLEMPERLWAIVQRAVLTCS"
     gene            1338..2135
                     /gene="cobM"
                     /locus_tag="VFQ61_28995"
     CDS             1338..2135
                     /gene="cobM"
                     /locus_tag="VFQ61_28995"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HET9958580.1"
                     /translation="MKVYIIGAGPGDPGLITVRGAELIARCKVVLYTGSLVPREILAR
                     APADAEVIDSSGLTLDQIIEVIVRAREAGLDVARVHTGDPTLFGSTAEQMRRLAELGI
                     EYEIIPGVSSFTAAAAVLGRELTLPELSQTVILTRAEGRTPMPERESLTALAQHQATM
                     CLFLSINLLKHVSESLIPAYGADCPVVVVHRASFPDQVVVRGTLADIRERVREAGLKS
                     QSMILVGRVLTSTDFADSKLYDPEFSHRFRRAEKNAKSAILPGSREP"
     gene            2132..3685
                     /locus_tag="VFQ61_29000"
     CDS             2132..3685
                     /locus_tag="VFQ61_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HET9958581.1"
                     /translation="MTAATLMIQGTASSVGKSIITAGLCRLLRNRGLRVAPFKSQNMA
                     LNAYVTEDGGEIGRAQAVQARAAGIEPTVDMNPILLKPEAESRSQVVVMGKPLGSLAA
                     VDYQARKGELREIVRGSLARLRSSYDVVVIEGAGSPAEVNLKPRDIVNMFVAEIADAP
                     VLLVGDIDRGGVFAAFVGTLELLEPGERARVAGFLINKFRGDVRLLDSGLEFLEQRTA
                     KRVFGVIPYVRDLQIADEDSVALEERRRRRRARRNEIEIAVVLLPSISNYDDFQPLEH
                     EPGVVVRFVEHPAELDGADLAVLPGTKSTLADLAYLRRSGFAEGLQARARAGEWVLGI
                     CGGCQMLGRNISDPAGVEASAPAQKQGLGLLSFDTEFGLRKRTTRTAFRVAIPNFLCE
                     SPEARLAGYEIHMGRLRPTLPERAAFRIQSRTGESDADAIALDGEVSSDGNVVGTMMH
                     GLFENEVVRRTLIGKLRARRGLPEVVPPEPANAYASAPVLAPDPFDRLADHLAAHIDT
                     RAIFRLIGR"
     gene            complement(3692..4642)
                     /locus_tag="VFQ61_29005"
     CDS             complement(3692..4642)
                     /locus_tag="VFQ61_29005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HET9958582.1"
                     /translation="MPGASLIALLDDIASVLDDVALLTKVATKKTAGVLGDDLALNAQ
                     QVTGVRAERELPIVWAVAKGSLVNKAILVPAALVISALAPWAVTPLLMLGGTYLCFEG
                     FEKIAHKFLHPAAEHAEAEEHVQRVARLSDPAVDLVAMEKDKIRGAVRTDFILSAEIV
                     TISLGTVAASPLPTRVAVLTAIGLVMTIGVYGLVGAIVKIDDLGAFLTRRAEATPGSG
                     VATLQAGLGRAILATAPYLMKGLSIAGTAAMFLVGGGILVHGIPGAHDAIHHALEGVL
                     GARGAALSVATTVVDALFGILVGALTLLVVSGVKRVVKRG"
     gene            complement(4834..8742)
                     /locus_tag="VFQ61_29010"
     CDS             complement(4834..8742)
                     /locus_tag="VFQ61_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958583.1"
                     /translation="MGAKNNGLRVGSRSIEGWAAPLLGWLLAACGTGDLDGNVSGSQQ
                     TGVLQQSVVEGAARPPQSRPRYTLFEAGPVRPIAVLDSGIVAVTNVPDDRLELFRSAR
                     SGVKPCGSVKVGMRPVAVAAVADRLWVVNHLSDSVSILKLDSEQCTAQLEKTLWVGDE
                     PRDIVSATSPKGERFVFVTSAHRGQNVTNADGSYRDPELTEPGRGRADVFVFGVDSQG
                     RSNAQAPLEILTLFTDTPRALAVGNGKVFAAGFMSGNQTAVARYQLVVDRGRQSLAKL
                     DQDGDLVMDASLPDNARVVEGGYPALQGHGRCLSGTTASEPGRDRTDFWMDVCVRTDP
                     QDGTRALEIVRQTAGVVTPECSCTNSLGELQMTPPLIVRFYESKEICGANYVAQLGGC
                     WFEPPHDEQDLAALAKNQRPPGMQAWNDAMAFTLPDRDVFTIDLNQSPPALVPSGEFR
                     HVGTNLLSMAVHPKTGKVFVGNTESRNHVRFEGPGEGTAQNARFANTTVRGHVAESRI
                     SVLDPASSATKPLHLNAHIDYSKCCAEAPNAESEQSLAFPVGLAITQKKNRSGQLSDA
                     QDLYVTALGSDKIAVLNTETLDKAQPGQIVQTRRDHIEVAGGPAGLALDEAQRRLYVF
                     THFTNELVVIDTNSRRELERLKMFTPEPESIVAGRPFLYDARRTSSHGDTACASCHLF
                     GDLDGLAWDLGAPNDRDFENTGPFFARPEFTSAPLTSRFLSVKGPMTTQSLRGMANHG
                     AMHWRGDRRGGLDSTVHAQPDTGAFDEQAAFKAFNVAFPGLNGRATQLTEAEMQHFTD
                     FVLALTYPPNPVRQLDDSLTEGQKRARSRYFGCEITDDSMSRRQCADGRSIEEETLNC
                     NCANPPEFVLGLVQRPAHCPKDPRCTLDVSDFQNTCHGCHTWNPAGNAEFGVDKPGFF
                     GTSGVYTNDSVSHVLKVPHLRNIYQKVGMFGSVQTKSGVGLNNLADSIFGPRQGGLLA
                     PKNAFLGDQIRGFGFTHAGEEDTIFHFFSSGGFARSASPGPGFINDNRGGFEPVLPAS
                     PEACFGSQLGSLNQRFIAELAAPEIIQQLQQQFAILTTSTSTPEQRAAAQQVLASFLL
                     SLPPSNPGAVFQRLPLAGAISQLALPLLACPRLPAYEQLAALGCFELKTGAGCADLIG
                     TVRGCALWGATLEQIVPNGTLACRAAGLDGKAEMESLMFAFDSDVKPSVGQQVTLTNK
                     PSAEAQARLRQLQAVADAQQLDLTATSQGRGYVYESGQLTRDDGTKVRVSTLLRKLDQ
                     YAPITFTAVPRGEGRRAGIDRDLDGVLDAFDLEVPGCNPH"
     gene            complement(9570..10208)
                     /locus_tag="VFQ61_29015"
     CDS             complement(9570..10208)
                     /locus_tag="VFQ61_29015"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958584.1"
                     /translation="MTDSYWLERWQQGRIGFHLTAPNANLVAHYSILQGSSRILVPLC
                     GKSVDLAWLHDQGHEVVGVDLSELAAQAFFEERGWTATRHQRGPFLVYTSGRMTFLVG
                     DFFQLSTEFLGSCDGAYDRAALIALPEPLRATYANKLISLLPSAARMLLVTLDFDVPG
                     GPPYAVSSEEVESLYGRERTEILSIREAGVEAPSAIDRGASYVREVAYSIQL"
     gene            complement(10270..10971)
                     /locus_tag="VFQ61_29020"
     CDS             complement(10270..10971)
                     /locus_tag="VFQ61_29020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958585.1"
                     /translation="MSETCQVRGARFACLAALSVSALLLGAKPAQAQQFGDPKTFTVG
                     VERLAGFVHSVSTVDTNPDTTVTSNTFTLLPFAGNALTVYSSPRLAFDYFVIQSLSIG
                     GSLGFGTFSRKQKVPVGVSTVETETVDTTGFLLSPRIGYGVMFSKHVGIWPRLGITYA
                     KFASAQDVEISALALSADVPLVIVPTRNIDIMIGLTGDLGLDGSLKSRQGNTSVSLDT
                     KTNELGVQAGFGVYF"
     gene            complement(11070..11555)
                     /locus_tag="VFQ61_29025"
     CDS             complement(11070..11555)
                     /locus_tag="VFQ61_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9958586.1"
                     /translation="MNIEIRRITEQELPKVAALAGQLLRQHHTTDPLRFFLPENPEQG
                     YEWWFARELRNPNVVLLAAVIETEVGAVEVVGYVYGRLEERDWNLLLDEHAAIHDLFV
                     APTARQSGVGGRLLETAISELAQLGAVRFVLSTMVSNAAAQKLFAKHGFRPTLLEMTR
                     G"
     gene            complement(11642..>12211)
                     /locus_tag="VFQ61_29030"
     CDS             complement(11642..>12211)
                     /locus_tag="VFQ61_29030"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET9958587.1"
                     /translation="LELGVLDDAESEMPWDGKTYSVPDWNRDHTLRTAMRVSCLPCFQ
                     SVARKIGPARLGDWVTRFAYGNHDTSGAPERFWLDGALRISPVQQIEFLRKLDEDKLP
                     IQKRTSEIVKDVITLDVGQNHVLRGKTGLLRPPEERQFFGWFVGWVELGPRRVYFATL
                     LDAVEPDVDPVPVRRRLTETILRSQQLLP"
BASE COUNT         2282 a         3713 c         3864 g         2352 t
ORIGIN      
        1 ggagtgagca cgtgacggac cagctttcaa ttcagcgagg tagtttgagc atcgtgggca
       61 tcggtcctgg cgctcacgca cacacgaccc cgcgggcgct cgaggcgatt gcccgtgcgg
      121 atgtcgtggt cgggtacgtg acgtacattc gtttggtcgc ggatctggtg caaggtaaag
      181 agatcgtgcg gaccgggatg accgaggaga tcgagcgcgc gcgggtggcc gtggagcgcg
      241 cacgtgccgg cgcgcaggtc gcgatcattt cctctggtga cgccggcgtc tacggcatgg
      301 ccgggttggt ctttcaagtc ttgaaagaga gcggctggcg ccgaggagac gcgccggagt
      361 tgcagctcat ccctggcatt accgcgctca acgcgtgcgc ttccttggtt ggggcgccgc
      421 tgggacatga tttttgcgcc atctcgctgt cggacctact gacgccttgg gcggtgattg
      481 aacggcgtat tcgcgcggcc gcggaatcgg acttcgtgat cggcctgtac aacccggcga
      541 gtggtcggcg cacgcgacag atcgttcgtg cgcacgagat aatcagcgag caccgcgcac
      601 ccgatacgcc cgttgcgctc gtcaagagtg cctatcggcg tcgcgaggaa cgggtgttga
      661 cggatctagc ccattgtctc gattacgaga ttggcatgct caccaccgtt atcgtcggct
      721 ctacgcagac cttcgagttc gagggctaca tggtgacccc ccggggttac gccaacaagt
      781 acacgtggga tggtgcgccg cggcctggtc agacaccggg ccgctcgcta atcgtagctt
      841 cgagcccggg agaggaaccg tgagcgcatg gttgggacgc atctccggtg cactcttgct
      901 tcagcgccca accccgcttc cggaaggcgc cgcgggcttc gagtattacc tgatcggaga
      961 cacgaagaga ccttgtaact gggaagccct gggcttcgtc gccccccccc aacgcgatcc
     1021 gctgcgcgag ccttggctga aactcgtgcc gctctccttt cagggattgt cgggaaagct
     1081 cccgttccag gggcccgtac ttcgtttgcc tcacgccgac caagccgccg tggaactact
     1141 ggctcggcgc ttgctcgtgc cgcgcaacgg ctcggtgagc gaacgcttgt ggcgcctcgt
     1201 tctgggcgca gaggatgacg aggacgtgct cgaatcccct tctgagttgg acgcagggtg
     1261 gctcctggaa atgccagaac ggctttgggc catcgttcaa cgagcggtgc tcacatgcag
     1321 ctgaccctag gggaaaaatg aaagtctata tcatcggtgc aggtcccgga gatccggggt
     1381 taatcacggt tcgcggggcg gagctgatcg cgcgctgcaa ggtggttctt tataccggct
     1441 cgctcgtgcc gagggaaatt ctggcgcgcg ctcccgcaga cgcggaagtc atcgattctt
     1501 cgggcctcac cttggaccaa atcatcgaag taatcgtgcg tgcgcgtgag gcagggctcg
     1561 atgtcgcgcg agtgcatacc ggggacccga ctctgttcgg atcgaccgcc gagcagatga
     1621 ggcgcctagc ggagctcgga atcgagtacg agatcatccc gggtgtgtca tcgtttacgg
     1681 cggcggccgc ggtcttggga cgtgaattga ccttgccgga gctctctcag accgtgattc
     1741 tcacgcgggc cgaagggcgc acacccatgc ccgagcgaga gagccttacc gcgcttgccc
     1801 agcaccaggc cacgatgtgc ctgtttctga gcatcaattt gttgaagcac gtgagcgaga
     1861 gcctcattcc ggcatatggt gccgattgcc cagtagtggt cgtgcaccgc gcgagttttc
     1921 ccgatcaggt ggtggtacgc ggaaccctgg ctgacattcg ggagcgcgtg cgggaggcgg
     1981 ggctgaaatc tcagtccatg attttggttg ggcgcgtact cacctctacc gatttcgcag
     2041 actccaagct ctacgatccc gagttcagtc atcgcttccg tcgggccgag aagaacgcga
     2101 agagcgcgat tttacccgga agtcgtgagc catgaccgca gccaccttga tgattcaagg
     2161 taccgcatcg tcggtcggta agagcatcat tacggctggg ttgtgccgct tgttgcgcaa
     2221 tcgaggcttg cgcgtcgcgc ctttcaagtc gcagaacatg gcactaaacg cttatgtcac
     2281 ggaggacggc ggtgagattg ggcgggccca ggcggtccag gcgcgcgcgg ccggcatcga
     2341 gccaacggtg gatatgaatc ccattctgct caaaccggag gccgagtctc gctctcaggt
     2401 ggtggtaatg ggaaagccgc tggggagtct ggctgccgtc gattatcagg cacgcaaggg
     2461 cgagctccgc gagatcgtac gcggctcgct ggcgcgactg cgctcgagct acgacgtggt
     2521 cgtcatcgag ggcgcgggca gcccggcgga agtgaacctg aagccgcgcg acatcgtgaa
     2581 catgttcgtc gcggaaatcg cagatgcacc ggtcctgctg gtgggcgaca tcgatcgggg
     2641 cggggttttc gcggccttcg tcggaaccct cgagttgttg gagcccggcg agagagcgcg
     2701 agtcgcgggc ttcctgatca acaagttccg cggggatgtg cggctcctgg attcgggtct
     2761 cgagtttctc gagcagcgca cggccaaacg cgtattcggt gtcatccctt atgtccgtga
     2821 tctgcagatc gccgacgaag actcggtggc gctcgaggag cggcgtcgac ggaggcgcgc
     2881 gcgacgcaac gagatcgaga ttgcggtggt gctcttgccg agcatctcca actacgacga
     2941 cttccagccc ctggagcacg aacccggcgt cgtggtgcgt ttcgtggaac acccagcaga
     3001 gttggatggt gcggatctgg cggtcttgcc gggtaccaaa agtactcttg ccgatctcgc
     3061 gtaccttcga cggtccgggt ttgccgaggg cttgcaggct cgtgcccggg cgggggagtg
     3121 ggtgctgggg atctgcggcg gctgccagat gctgggtcgc aatatcagtg acccggcggg
     3181 ggtggaagcg agtgcaccgg cgcaaaaaca aggtctcggt ctcctgtcgt tcgacacgga
     3241 gttcggacta cgcaagcgca ccacgcgcac cgcgttccgg gtcgcgatcc cgaactttct
     3301 ctgcgaatca cccgaggcgc ggctggctgg ctacgaaatc cacatgggcc gcctgcgccc
     3361 gaccctgccc gagcgagccg cctttcggat tcagtctcga acgggagaat ctgacgctga
     3421 tgccatcgcg ctggacggcg aggtctcgag cgatggcaac gtcgtgggga cgatgatgca
     3481 cggcctgttc gaaaacgaag tggtgcgccg cactctaatc ggcaagctcc gcgcacggcg
     3541 cggccttccc gaagtcgttc cacccgagcc ggcgaatgct tacgctagcg ctccggttct
     3601 cgcacctgac ccgttcgatc gcttggcgga tcatctcgcg gcgcacatcg acacgcgagc
     3661 catctttcgc ctcattggac gatgagacgg ttcaaccacg cttcacgacg cgtttgacgc
     3721 cgctcacgac gagtagcgtc agcgcgccga cgaggattcc gaagagcgca tccacgaccg
     3781 tcgttgccac cgacaacgcg gcgcctcttg ctcccaacac gccttccagc gcatggtgaa
     3841 tcgcatcgtg cgcgccaggg atgccgtgca cgagaatgcc cccgcccacc aagaacatgg
     3901 cggccgtgcc cgcaatcgag agccccttca tgagatacgg cgcggtcgcg aggatggcgc
     3961 ggccgagtcc ggcttgcaga gtagctacgc cgcttccagg ggtcgcctcc gcgcgacgag
     4021 tcaggaacgc gccgagatca tcgattttta cgattgcgcc tacgagcccg taaacaccta
     4081 tcgtcatgac cagaccgatg gccgtgagta ccgctacgcg ggtgggcaga gggctcgcgg
     4141 ccaccgtccc gagcgagatg gttacgattt cggcggatag aatgaagtcg gtccgcacgg
     4201 cgccgcggat cttgtccttc tccatcgcca ccaagtcgac ggccgggtcg gagaggcgcg
     4261 ccacacgttg cacgtgctct tccgcttctg cgtgctccgc ggcaggatgc aaaaacttgt
     4321 gggcgatctt ctcgaagcct tcgaagcaca agtaggttcc gccgagcatg agcagaggcg
     4381 tgacggccca cggcgcgagc gcgctgatca ctagcgctgc gggcaccaga atagccttgt
     4441 tcacgagcga gcctttggct acggcccaga caatcggcag ctcccgctcg gcgcgcaccc
     4501 ccgtaacctg ctgtgcgttc agcgcgaggt catcaccgag cactccggcg gtcttcttgg
     4561 ttgcgacttt ggtgagtagc gcgacgtcat cgagcacact ggcaatgtca tcgagcaggg
     4621 caatcaggct ggctccaggc atcacctccc ttcctctcgc gagcggggcg ccacgtcaac
     4681 cctcccgggg atacgtgcaa acgacagacg ggagggccga atgaccctcc cgcctcgtcc
     4741 gagcgcagga aacgtagtga cttgttcgaa accagccggt ttcgagccgg cgcttcgagc
     4801 gggcaccgtt cgcgcaccgc gctcaaacgc tgattagtgt gggttgcagc cggggacctc
     4861 taggtcgaat gcgtccagga ccccatccag gtcgcggtcg attcccgcac ggcgaccttc
     4921 cccgcgtggc accgcagtga aggtgatcgg tgcgtactga tccagcttgc gcaacagcgt
     4981 actcacacgg accttggtgc cgtcgtcgcg cgtcaactga cccgattcgt agacataacc
     5041 gcgcccctgt gaggttgcgg tgaggtccag ctgctgagcg tcggccaccg cctgaagctg
     5101 tcgcaaacgg gcttgcgctt cggccgacgg cttgttggtg agcgtgacct gttggcccac
     5161 gcttggcttg acgtcgctgt cgaaagcaaa cattaggctt tccatctccg cctttccatc
     5221 caacccggcg gcgcggcacg cgagggttcc gttcggtacg atttgctcca gggtggctcc
     5281 ccagagcgcg caaccccgca cggtaccaat caagtccgcg catccggctc ccgtcttcag
     5341 ctcgaagcag ccgagcgcgg ccagttgctc gtaagcaggg agccgggggc aggcgagcaa
     5401 cgggagggcc aactggctga tggcacctgc caacggcaag cgttggaata ccgcgccggg
     5461 gttgctcggc ggcagggaca gcaagaagct cgctagcact tgctgtgcgg ctgcacgctg
     5521 ctccggcgtg gatgtgctgg tcgtcaggat ggcgaactgt tgctggagct gttgaatgat
     5581 ctcgggcgcc gcgagctcgg caatgaagcg ttggttgagc gagccgagct ggctaccgaa
     5641 gcatgcctcg ggagaggcgg gcaacacggg ttcgaatcca ccgcgattgt cgttgatgaa
     5701 gccgggaccg ggcgatgccg agcgcgcaaa acctcccgag gagaagaagt gaaatatcgt
     5761 gtcctcttcg cccgcgtgag taaacccgaa gccgcggatc tgatcgccca agaaggcatt
     5821 cttcggggcg agcaaaccac cctgacgggg cccaaagatc gagtcggcga ggttattgag
     5881 ccctaccccg gacttggtct gcacggagcc gaacataccg accttctggt agatgttgcg
     5941 caggtggggc accttcaaga catgtgagac ggagtcattg gtgtacacgc cgctggttcc
     6001 gaagaagccg ggcttatcga cgccgaactc ggcatttccg gcggggttcc aggtgtgaca
     6061 gccgtgacag gtattctgga aatcgctcac gtcgagagtg catctcggat ctttggggca
     6121 atgggcgggc cgttgcacca gtccgagcac gaactcgggc ggattcgcac aattgcagtt
     6181 cagggtctct tcttcgatgc tgcggccgtc ggcacattgc cgtcgactca tcgagtcatc
     6241 ggtgatctcg cagccgaagt agcggctcct cgcgcgcttt tgcccttcgg tgagcgaatc
     6301 atccaattgc cggactggat tcgggggata ggtgagagcc aaaacgaagt cggtgaagtg
     6361 ctgcatctcc gcctccgtca actgggtggc tcgaccgttc agcccgggga aggcaacgtt
     6421 gaacgctttg aatgcagctt gctcgtcgaa ggctcccgta tccggttgcg cgtgcacggt
     6481 gctatcgaga ccccctcgtc ggtcaccacg ccagtgcatt gcgccgtggt ttgccatgcc
     6541 gcgcagcgac tgcgtggtca tcggcccctt gacggacaag aagcgcgacg tgagcggtgc
     6601 gctcgtgaat tctgggcgtg cgaagaacgg gccggtgttc tcgaagtcgc gatcgttcgg
     6661 ggcgcccaag tcccaggcca gcccgtcgag gtcgccgaac aaatggcagc tcgcgcaagc
     6721 cgtatcgcca tggctcgagg tgcggcgcgc gtcgtacaag aatgggcgtc cggccacgat
     6781 cgattcgggc tcgggcgtga acatcttgag acgctccagc tcgcggcggc tgttggtatc
     6841 gatcaccacc agctcgttcg tgaagtgggt gaatacgtag agtcgacgct gcgcttcgtc
     6901 gagcgcgagc ccggcggggc cgccggcgac ttcgatatga tcccggcgag tctgaacgat
     6961 ttgacccggc tgggctttgt ccagtgtctc ggtgttcagc accgcgatct tgtcggaccc
     7021 caaagcggtt acgtacaagt cttgcgcgtc actcagctgc ccggagcgat tcttcttctg
     7081 agtgatggcg aggcccaccg ggaaggcgag gctctgctca ctctccgcgt tgggggcctc
     7141 ggcacaacat ttggagtagt cgatgtgcgc attcaagtgg agtggcttgg tggcgctact
     7201 tgccggatcc agcacgctga tccggctctc ggcgacgtgc ccacgcacgg tggtgttggc
     7261 gaatcgcgcg ttctgggccg ttccctcgcc aggaccctcg aagcgaacgt gattgcggga
     7321 ctccgtattg cccacgaaga ccttccccgt cttcgggtgc acggccatgc tcaaaaggtt
     7381 cgtgccgacg tgccggaact ccccactggg aaccagcgcg gggggagact ggttcaagtc
     7441 gatggtgaac acgtcgcgat cgggaagcgt gaacgccatc gcgtcgttcc aggcttgcat
     7501 tccgggcgga cgctgatttt tcgcgagcgc tgcgagatct tgctcatcgt gcggtggctc
     7561 gaaccaacaa ccaccgagtt gagcgacgta gttcgcgccg cagatctctt tggactcgta
     7621 gaatcgaacg atcaagggtg gagtcatctg caactcacct agcgagttgg tgcaggaaca
     7681 ctccggtgtg accacccccg cggtctgccg aacgatttct agggcccggg ttccgtcctg
     7741 tggatcggtg cgaacgcaga cgtccatcca gaagtcggtc cgatcccggc ctggctcact
     7801 cgctgtcgtg ccggataggc aacgcccgtg gccttgcaac gccggataac cgccctccac
     7861 gactcgcgcg ttgtccggta ggctggcgtc catcacgaga tcgccgtctt ggtctagctt
     7921 agccaagctt tggcgcccgc gatcgacgac cagttggtaa cgtgcgacag cggtttgatt
     7981 gccggacatg aacccggccg caaagacttt accgttgcca accgcgagcg cgcgaggcgt
     8041 gtccgtgaac agcgtgagga tttcgagtgg agcctgggcg ttggatcggc cctgggaatc
     8101 caccccgaaa acgaagacat cggctcgacc gcgcccaggt tcggtcagct cgggatcacg
     8161 gtagctcccg tccgcgttag tgacgttctg gccgcgatgg gcggaggtca cgaaaacgaa
     8221 tcgctccccc ttggggctcg tcgcagacac gatgtcccga ggctcgtcac ccacccaaag
     8281 agtcttctcg agttgcgcgg tgcactgctc cgagtcgagc ttgaggatgc ttacactatc
     8341 cgataggtga ttgacgaccc acaaccggtc cgcaaccgca gccactgcca ccgggcgcat
     8401 cccgaccttg accgagccgc aaggcttgac gccgcttcgc gcgctgcgaa acaactcgag
     8461 tcgatcgtcg ggaacgttcg tgaccgcgac gattccgcta tccaacacgg cgatgggtcg
     8521 tacggggcct gcctcgaaca aggtatatcg aggacgcgat tggggtggac gagccgcgcc
     8581 ttcgacaacg gactgctgca agacgccagt ctgctgcgac cccgagacgt ttccatccag
     8641 atcgccagtc ccgcacgctg ccagtaacca tccgagcagc ggggctgccc agccttcgat
     8701 acttctcgat cctacccgta atccgttgtt ctttgcgccc atgtgacacc ttcagtttcg
     8761 attgtgcgag gcaaatcctt ggagggaggc gggcagagcc gagccgcgag ctcggcgcac
     8821 taaagtcgct tagacatgcc aatcggttca gaccgttgac ccaaaacagc taattttgcg
     8881 cctctcgaac caatcgtcgc ccacggaact agccccttgc ctagtgcttc gggtgatgcc
     8941 cctatcgatt cttgagatgc gcgcgtgaga cgtaagatct cgagatgtga actagtgcgc
     9001 ccgaccaaga tgcgtgtgcc ggagctggtg cccttctgct cgacgagccg gccacgactg
     9061 ccggcaggcg gggccgaagc tccgcctaag ttcctctaca aacccgagct ccggggctgc
     9121 aggcccgggt cgctcacctt cctcgttttg catctcgtca ttcggcgcac aaactgtcaa
     9181 ttgctttgat gcaacccttc aggatttgta agagcaccac tcctacggat aatgaaactt
     9241 tccgagtgag ctccgcaaaa gcgctcaagc ggcagtcgag caaaggaggc aatgtgggtg
     9301 tgagtcgctc caggcaatgg gtgtgttgcg agagctcgtg aaactgaact cgcagccctc
     9361 gatgcacggg ggctcgcata aacctatttc acgctcggcg cagcagcgct gcacggaggc
     9421 gctactggcg tgcgcggggt gctggtctcg ggtgctgcgc tggcccgtgc accggcaaag
     9481 agcaaaccct cagcggccgc agctcgcggc gtttgcgctt gcgccccctg cggccgggga
     9541 tggacccgga aggcggaccg ggaggattgt caaagttgga tcgagtaggc gacttctcta
     9601 acgtaactgg caccccggtc tatggcggag ggggcctcga cgcccgcttc tcgaatgctc
     9661 aagatttccg tccgctcgcg gccatacagg ctttcgactt cctccgaaga taccgcgtag
     9721 ggagggccac ctggcacgtc gaagtcgagc gtcaccagaa gcatgcgcgc tgcagacggg
     9781 agtaggctga tcaacttgtt cgcatacgtg gcgcgcagcg gctccggcaa ggcgatcaag
     9841 gccgctcgat cgtaggcgcc gtcacaggaa ccgagaaatt cggtgctcag ttggaagaag
     9901 tcgcccacca gaaaggtcat acggcccgaa gtgtagacca ggaaggggcc tcgctgatgg
     9961 cgagtggctg tccaaccgcg ctcctcgaag aacgcctgcg cggcgagttc actcaggtcc
    10021 actccgacga cctcgtggcc ctgatcgtgt agccaagcca gatccacgga cttcccgcac
    10081 aggggcacca gaatccgcga gctaccttga agtatcgagt agtgggcgac taggttcgcg
    10141 ttcggggccg tgaggtgaaa gccgatacga ccttgctgcc agcgctccaa ccagtagctg
    10201 tccgtcacgt ttcgaaaggt agcgccgaga atgaacgttg gctagctctc gggcagcggt
    10261 gtccggggct tagaaataga ctccgaagcc ggcctgtacc ccgagttcgt tggtcttcgt
    10321 gtccaggctg acggaagtgt tgccttgtct agatttgagg ctgccgtcta gccccaagtc
    10381 cccggtcaag ccaatcatga tatcgatatt gcgcgtcggc acgatgacca gcgggacgtc
    10441 ggccgataac gcgagcgcgc tgatctctac gtcctgagcc gacgcgaact tcgcgtaagt
    10501 gatgccgagg cgcggccaaa tgcccacgtg cttgctgaac atgaccccat atccaatccg
    10561 gggcgaaagc aagaaacccg tggtgtctac cgtttcggtt tcgacggtgc ttactcccac
    10621 aggcactttc tgttttctgg aaaaagtgcc gaagcccaat gacccgccga tactcaggct
    10681 ttggatgacg aagtagtcga acgcgagccg aggagaggaa tagaccgtga gtgcgttgcc
    10741 ggcgaacggc aagagcgtga aagtgttgga tgtcaccgtc gtgtcgggat tggtatccac
    10801 cgtcgacacg ctgtgtacga atccggcgag tctctcgaca cccaccgtga acgtcttggg
    10861 atcgccgaac tgctgagctt gggctggctt cgcgccgagt agcagggcac tcacggacag
    10921 cgccgccaag catgcaaatc gggcaccacg gacctggcac gtttcgctca tcgacgcgag
    10981 cttcgcacac cctttgctcg atgggcaagt tgcactgtac cgagcccgag ctacctagcc
    11041 cgagctctga accagcctgc tcctcagcct caaccccggg tcatttcgag taacgtaggg
    11101 cgaaaaccgt gtttcgcgaa caacttctgc gcggcggcgt tgctcaccat ggtcgaaagc
    11161 acgaaccgaa cggcgccgag ttgggctagc tccgagatcg cggtctccag tagccgtcca
    11221 cctactccgg attggcgcgc ggtgggcgca acgaacaagt cgtgaatagc ggcgtgctcg
    11281 tcgagcaaca aattccagtc gcgttcttcc aggcgaccgt acacgtaacc taccacctct
    11341 accgctccca cttcagtctc gatgacggcc gccagcaaca cgacgtttgg attgcggagt
    11401 tctcgcgcga accaccactc gtatccttgt tctggattct ccggcaagaa gaaacgtaga
    11461 ggatccgtgg tgtggtgctg tcgcaatagc tgtccagcta gtgcggcgac cttgggcaat
    11521 tcctgctcgg tgatgcgacg gatttcgatg ttcatgactc gatatttcaa gagagtcgag
    11581 agtgggtcag tgctgctgtt gataactcgt gtcaactatc gtcgcgacga gcatacgagc
    11641 ttcagggcag cagttgctga gagcgtagga tcgtctcggt gagtcggcgt cgcacaggca
    11701 ccggatcgac gtctggttcg actgcgtcga gcagcgtcgc aaaatagacc cgacggggac
    11761 cgagctctac ccaacccacg aaccatccga aaaactggcg ttcctccggg gggcgcagca
    11821 ggcctgtttt cccccgcaag acgtgatttt gcccaacatc caacgtgatg acgtctttca
    11881 cgatctcgct ggtgcgcttc tgaatcggaa gcttgtcttc gtccagcttc ctcaaaaatt
    11941 cgatttgttg aaccggagaa atacgcaaag caccgtccaa ccagaaacgc tcgggggcgc
    12001 ccgaagtgtc atggttgccg taggcgaacc gggtgaccca gtcccccaaa cgcgccgggc
    12061 cgattttgcg cgcgacgctt tgaaagcacg gcaaacacga gacacgcatc gctgtacgca
    12121 gcgtgtggtc gcggttccaa tctggaacgg agtacgtctt tccgtcccac ggcatttcgc
    12181 tctccgcatc gtccaacaca cccaactcga g
//
LOCUS       DASTKU010000829         3592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1056090, whole genome shotgun sequence.
ACCESSION   DASTKU010000829 DASTKU010000000
VERSION     DASTKU010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056090"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFQ61_29035"
     CDS             complement(<1..395)
                     /locus_tag="VFQ61_29035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958588.1"
                     /translation="MRRNRGLTRRDLIRCFGPLAFLLMPVTRAMGYVAGGSFAGAPRF
                     VLFFKGPSFHSPTVNPTKSIDSLPAPLAPLQPHAQDIILFSGMSIHGGSPKTDGYQEE
                     HAAGLIGCSTGNSYHYSKNDSYYAYTDNES"
     gene            complement(395..2200)
                     /locus_tag="VFQ61_29040"
     CDS             complement(395..2200)
                     /locus_tag="VFQ61_29040"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET9958589.1"
                     /translation="MRRVCLRLPIAVVPRALALALLGCDATIIESPSGSAGASGAGAV
                     TPGGLGGGANGGGTASNGAAANSGGLSSASGGSAGGGVNGGVSSAGGNKPTLEEVAKR
                     YFPSDNAVNANKRLSRLTRMQLDLTTKTLLPKQFAATASASLPRDPLQTNYEYADNLS
                     FNPANFSPFINWVAGMSAAVKADPRSVIDCKSSNDSVECLTDRAKRFVNLAFRGTASE
                     AQLNRYAEFFTGSVATVGLPTATAELVDLVLTSPNYVFRDEVATDGTGALLPAQQLQN
                     ITYTLADAPPDALGLSSATPLKQLSSPAVVQSTIDQVLATPEARNKLLRFFLAWLEVK
                     EPAEFGIASSVFPEFTEEVATAVVAETAAFLEHQLARAAPSMKDITESTESFATNAGA
                     FLYGNAASKQLDPAQRLGIFTQPAVLASHSGPTTSRLVKRGVFFVRKIMCMPLGSPPP
                     NVDISISPSAGKTERERIENGTAKAPCNGCHGVINPFGFMQENFDAIGRWRTSDEGVP
                     IDPHIEVNFLDEGPFTANTPVEALRTFTRSWRFQQCFARQLFRFYSGRDETAGDDPLL
                     RQMFLEFANDNGQDIVRMLHTLASSSAFSRRSEVQ"
     gene            complement(2354..3202)
                     /locus_tag="VFQ61_29045"
     CDS             complement(2354..3202)
                     /locus_tag="VFQ61_29045"
                     /inference="COORDINATES: protein motif:HMM:NF015500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HET9958590.1"
                     /translation="MSCALHKATALVCALVLLPETLAACTGTEGDLLRTRPETSDPGD
                     PATPRPRPNSLSSWQIQLSGSLDTTLDVELYTADLETPVAAIQALHQAKRIVICYFSA
                     GTREPFRDDAALFPASSVGAPLVDYPNERWLDIRDATVRSIMRERVARAAEIGCDGLH
                     PSGLGAFQEDTELDLSRADQLAYNRWLASEAHGLGLSIGLVEGDTEARQELVADFDWT
                     VVWSCVESGCLAAAPFLTAAKPAFVIEYGDAARAAELCPRAQELGLSAVIKRNADLDA
                     YRAGCP"
     gene            3247..>3592
                     /locus_tag="VFQ61_29050"
     CDS             3247..>3592
                     /locus_tag="VFQ61_29050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958591.1"
                     /translation="MLAIQLLLVRGSPAPLTARELGAAPFQIARPSPAVPHCGVSSGA
                     RVRELQSLSAVVSVERALDVERTLGGAEAESALPLEFDEVFRRYAPYVGALVLKLIGR
                     PGDVDDVVQDVFI"
BASE COUNT          638 a         1065 c         1214 g          675 t
ORIGIN      
        1 gactcgttat cggtgtaggc gtagtaggag tcgttcttgg agtagtgata actattgccg
       61 gtggagcagc cgatcaaacc cgccgcgtgc tcctcttgat aaccatcggt tttgggggaa
      121 ccgccgtgga tgctcatgcc cgaaaagagg atgatgtcct gggcatgcgg ctggaggggg
      181 gcgagcggcg ccgggaggct atcgatcgac ttggttgggt tgaccgtagg cgagtggaac
      241 gaggggccct tgaaaaacaa tacgaagcgc ggcgccccgg cgaaggatcc ccccgcgacg
      301 tagcccatgg ctcgcgtgac cggcatcaac aagaaggcga gcggtccaaa gcagcggatc
      361 aaatcgcgac gggtgagccc acggtttcgg cgcatcactg gacctccgaa cggcgtgaaa
      421 aggcagagga gcttgccaac gtgtgcagca tgcgcacgat gtcttgcccg ttgtcattgg
      481 cgaactcgag gaacatctgt cggagcaacg ggtcatcgcc cgccgtctcg tcgcggccgc
      541 tgtaaaagcg gaacagctgc cgcgcgaagc attgttgaaa gcgccaagag cgcgtgaagg
      601 tgcggagtgc ttccactggc gtgttcgccg tgaacgggcc ttcgtccaag aagttgactt
      661 cgatgtgggg atcgatgggc acgccttcgt cgctggtgcg ccagcgccca atcgcgtcga
      721 agttctcctg catgaagccg aaggggttga tgacgccgtg gcagccgtta cacggagcct
      781 tggcggtgcc gttttcgatg cgttcgcgct cggttttccc cgcgctgggc gagatgctga
      841 tatcgacgtt gggcggcggc gagccgagcg gcatgcacat gatcttgcga acgaagaaca
      901 cgccgcgttt gacgaggcgg ctcgtggtcg gacccgagtg ggaggcgagc acggcgggct
      961 gggtaaagat gcctaggcgt tgcgcggggt cgagctgttt ggaagcggcg ttaccgtaga
     1021 gaaacgcgcc cgcgttggtg gcaaacgact cggtggactc ggtaatatcc ttcatgctcg
     1081 gtgccgcacg ggctaactgg tgctccagga acgcggcggt ctcggccacg accgccgtgg
     1141 ccacttcttc cgtgaactcg gggaatacgc tgctagcgat cccaaactct gccggctctt
     1201 tgacctccaa ccaagctagg aaaaatcgga gtagcttgtt gcgggcttcc ggcgtcgcga
     1261 gcacttgatc gatggtgctt tgaacgaccg cggggctcga aagctgtttg agcggggtag
     1321 cggaagagag tcccaaggcg tcgggcggtg cgtctgctag cgtgtacgtg atgttctgga
     1381 gttgctgggc gggcagcaac gcacccgtgc cgtctgtcgc gacttcgtcg cgaaatacgt
     1441 agttgggaga ggtcaaaacc aggtcgacca actccgcagt ggccgtcggg aggccgacag
     1501 tggccacact gccggtgaag aactcggcat aacgattcag ctgagcctcg gaggcggtgc
     1561 cgcgaaaagc taggttcacg aagcgcttgg ctcggtcggt caaacactcg accgagtcat
     1621 tgctggattt gcagtcgatc acgctgcggg gatcggcctt gacggcggcg ctcatgccgg
     1681 cgacccaatt gatgaacggg ctgaagttcg ccgggttgaa gctgagattg tcggcgtatt
     1741 cgtagttggt ttgcagcgga tcgcgcggca gactcgcgct ggcggtcgcg gcaaattgct
     1801 tgggtagcag cgttttggtg gtgaggtcga gctgcatgcg ggtcaagcga gagagacgct
     1861 tgttcgcgtt caccgcgttg tcagaaggga agtagcgctt ggcgacttct tcgagcgtgg
     1921 gcttgttgcc gcctgcactg ctcactccac cgttcactcc acccccagcg ctgccgccac
     1981 tcgcgctgct caatccaccg gagttagcag ccgcgccatt ggacgcggtt ccgcccccat
     2041 tcgcgccgcc gcccaagccg ccaggggtga cagcacccgc gccgctagca cccgcgctgc
     2101 cggatgggct ctcgatgatg gtcgcgtcgc aaccgaggag cgccagcgcc agcgctcgcg
     2161 ggacaacggc tatcggcaaa cgcaggcaga ctctacgcat agacgcgctc ctcattcggc
     2221 agatctccgg gttcgttctg ccagtggcag cgacctccct gatcctggtc agcccttcag
     2281 agattttttg gatctcgcac agcgaggacc gcatcgacgc gtgcggcccg tggcgcccgc
     2341 cccgagcaat ccgttacgga cagccagcgc ggtaggcgtc gagatctgcg tttcgcttga
     2401 tcaccgcaga gagcccgagc tcctgggcgc ggggacaaag ctccgcggcg cgcgcggcgt
     2461 ccccatattc aatcacgaac gcaggcttgg ctgcggtcag aaagggagcc gccgcaaggc
     2521 agccgctctc gacgcagctc cacaccacgg tccagtcgaa gtctgcgacc agctcttgtc
     2581 tcgcctcggt atcgccctcg accaggccga tagaaaggcc cagcccgtga gcttcgctcg
     2641 cgagccagcg attgtaagcg agctgatcgg cacgcgagag gtcgagctcg gtatcctctt
     2701 ggaacgcacc cagaccactc ggatgaagcc cgtcacagcc gatctctgct gctctcgcga
     2761 cgcgctcgcg catgatggat cgaaccgttg cgtctcggat atccagccaa cgctcattgg
     2821 ggtaatccac cagcggtgct ccaacactac tcgccgggaa gagcgccgcg tcgtcgcgaa
     2881 aaggctcgcg cgtgcctgcg ctgaagtagc agattacgat gcgcttcgct tgatggagcg
     2941 cctggatcgc cgcgaccggt gtctcgaggt cagcggtgta aagttccacg tcgagcgtgg
     3001 tatcgagcga tcccgacagt tggatctgcc agctgctcag cgaattcggc cgagggcgcg
     3061 gagtggccgg gtcgcccgga tccgaagttt cgggccgagt acgcagcaag tccccttcgg
     3121 ttcctgtaca cgcggcgagc gtttcgggca gtagtactag cgcgcacacg agcgcagtgg
     3181 ctttgtgaag ggcgcacgac attccgtgac caggttttgc gcgttcgggg ccactgggca
     3241 aatggcttgt tggcgattca acttctattg gttcgcgggt cccccgcccc gctcacggct
     3301 cgcgagctgg gggccgcgcc tttccagata gcgcggccta gccctgccgt gccacactgc
     3361 ggcgtgtcga gtggcgcccg cgttcgagag ctgcagagcc tgtccgcggt ggtgagcgtg
     3421 gagcgagcgc tcgacgtgga gcgaacgctg ggcggggccg aagccgagtc ggcgctgccg
     3481 ctggagttcg atgaagtgtt tcgtcgctac gcgccctacg tgggagcatt ggtgctcaag
     3541 ctcatcggtc gtcctgggga cgtggacgac gtggtgcaag acgtgttcat tc
//
LOCUS       DASTKU010000830         4676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1950888, whole genome shotgun sequence.
ACCESSION   DASTKU010000830 DASTKU010000000
VERSION     DASTKU010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950888"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFQ61_29055"
     CDS             complement(<1..627)
                     /locus_tag="VFQ61_29055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958592.1"
                     /translation="MPPRSLRIRELPDRRHWLESLWGQARIGLRGLSRPIQVMATLVQ
                     LGWLQLSWVQLGCAGASAPGTEAAGRQLRGHSDPMNIGPKDGDRTSAIASAQGSNAAE
                     STSGRAAPPSSPELPSAASQEPTVAPPDATPAQASASIPFSIEERDELGRLPRPSGGM
                     AASEFDVELERWNAGGNSEPTRPSSRPSYHPATRVLVEIGALSRRLVSA"
     gene            862..2598
                     /locus_tag="VFQ61_29060"
     CDS             862..2598
                     /locus_tag="VFQ61_29060"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9958593.1"
                     /translation="MVQDVFGIVGSVIADSYEIEAPVAEGGFAVVYRAHHRAFRAKVA
                     LKCLKLNPSLAEYGRKLFLEQFRAEAEILYQLSARLHEVVRPLHVDALTTKSGQFVPF
                     LVLEWLDGDTLDVWVRKRTATGTRPLGIRRLVRLLTPVARALDTAHHFVGSRGASRVV
                     HCDMKPHNVVMARAGNDELPKILDFGIARVRRFAEPLGAGEKVPHSFTPRYASPEQWL
                     PKRYGSFGPWTDVWGLALTLVELLLPSSSLVGDAMSMALDPKQRPTPRAFGVEIPEPA
                     ERVLTQALAIDPQARYRDVASFWDELLQALELEPGVATGTLPRDQRREAGRVIQVERL
                     ETSALQVPAARPETAQHPREVPAAMNPSLPRAPFPFLSVHPAPIAAGAQPVGQLVRDT
                     HRELASALRPRRNDDVRIQPIEIPRAASLPRDLFAALHEATARESAAPAANTIPTPGL
                     PAEEITEELHLRIDRCASEVVPAGLDSILLESGTHFQSPNTHPSWASALAATSSAPPD
                     ENQAPPNCTAAPLDRAVRDDGAVHGYMVARNYHAPAGGQQAGWVRRAGLVLMCAVLAL
                     LGGYIGARFGQP"
     gene            2843..3172
                     /locus_tag="VFQ61_29065"
     CDS             2843..3172
                     /locus_tag="VFQ61_29065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958594.1"
                     /translation="MPKRPAQTAVLDPVEQLVARARRMRSKGEAKRSLTLLREACCLA
                     EQDARLWTLYSIQCWRLGQRNEGRRALRQALWLRERERDESRVRVLKQLLLAMESACA
                     PDELRAA"
     gene            4213..4285
                     /locus_tag="VFQ61_29070"
     tRNA            4213..4285
                     /locus_tag="VFQ61_29070"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4246..4248,aa:Gly,seq:ccc)
     gene            complement(4380..>4676)
                     /locus_tag="VFQ61_29075"
     CDS             complement(4380..>4676)
                     /locus_tag="VFQ61_29075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958595.1"
                     /translation="EGYGRPLSPRPRGKLSSGGQVVSQETELPRLSVDECVRYTLTLD
                     PDVALLGMSFPNEQDAALRAARDFEPLNPSALQEIRSRAELAMRGKGECWWNPR"
BASE COUNT          837 a         1481 c         1515 g          843 t
ORIGIN      
        1 ggcactcacc agtcgccggg aaagcgcacc gatctccacg aggacgcgtg ttgccggatg
       61 gtaactcggg cgcgaacttg gacgcgtcgg ttctgaattg ccaccggcgt tccaacgctc
      121 caattctacg tcgaactccg atgccgccat accgccgctc ggcctgggca accggccgag
      181 ctcgtcgcgc tcctcgattg aaaaagggat gctggcggac gcctgggctg gcgttgcatc
      241 gggcggggcc accgttggtt cctggctggc ggcgctgggg agctcggggg aagacggggg
      301 agcggcccgc ccactcgtcg attcagcagc gttcgagcct tgcgccgaag cgatcgcaga
      361 cgttcgatcg ccatcctttg gcccgatatt cattggatcc gagtgcccgc gcagctgccg
      421 cccagctgct tctgtacccg gcgcagaagc gccagcacaa cccaactgga cccaactcag
      481 ctgtagccag cccagctgaa ctaaggtggc cattacctga atcgggcggc tcaacccgcg
      541 aagcccgatc ctggcttggc cccacaggct ttcgagccag tgccgccgat cgggtagctc
      601 tcggattcgc aaagaacgcg ggggcatcga aaaactcact actgcgcgaa gcgtaaatct
      661 gaaacgacag gctgaactac ctttggaaag cggcgcttct ggaatttcgc gaagcaatat
      721 cagaaccttc gaaatgcagg gtgtgtcgcg aacgggcggg ttcgaaagat gatatgttca
      781 cgcgcagttt agggttccgg aggacagctt ccgcagcgct gctaggagca tcggcggtcg
      841 aactttaggt caagcgcgcg catggttcag gacgtgttcg gtatcgtcgg ctcggtcatt
      901 gccgacagct acgagattga ggccccggtc gcggagggag gattcgcggt cgtgtatcgg
      961 gctcatcatc gcgctttccg ggccaaggtt gcgctgaagt gtttgaagct caaccccagt
     1021 ctggctgaat acggtcgaaa gctgttcttg gaacagtttc gagcggaggc ggagatcctg
     1081 tatcagctgt cggctcgact gcacgaagtg gtgcgacccc tccacgtgga cgcgctcacc
     1141 accaagtcgg gccagttcgt gccgttcctg gtgctcgagt ggttggacgg agatacgctg
     1201 gacgtttggg tgcggaaacg aaccgccacg ggcactcgac cgctcggcat tcgacggctg
     1261 gtgcgcctgc tcacgcccgt cgcgcgggcg ctggataccg cgcatcattt cgtgggctcg
     1321 cgaggtgctt cccgcgtagt tcactgcgat atgaagccgc acaacgtggt gatggctcga
     1381 gcaggaaacg acgagctgcc gaagatcctg gatttcggaa tcgctcgcgt gcgccgattt
     1441 gccgagccgc tcggcgccgg agagaaggtg ccgcactcgt tcactccgcg ttacgcttct
     1501 cccgagcagt ggttgccgaa acgctatggc tctttcggcc cttggacgga cgtttggggg
     1561 ttggcgctga cgttggtcga gctgctcctg cctagctcga gcctggtcgg agacgccatg
     1621 agcatggcgc tcgatccgaa gcagcgaccc acgccgcgcg ccttcggggt agagattcca
     1681 gagcccgccg agcgtgtctt gacccaagcc ttggcgatcg atccccaagc gagatatcgg
     1741 gacgtagcga gcttttggga cgagctcttg caagcgctcg agctcgaacc gggagtggcg
     1801 accggaacgc tcccgcggga ccagcggcgc gaggcggggc gtgtgattca agtcgaacgc
     1861 ctggagactt cggctctcca ggtccctgct gcgcgtccag agacggcgca acacccgcgt
     1921 gaggtgcccg cagcgatgaa cccgtccctc ccgcgggcgc cgtttccatt tctctctgtg
     1981 caccccgcgc caatcgcggc cggcgcacag cccgtcggac aactcgtgag ggatacgcac
     2041 cgcgagctcg cctccgcact gcggccccgc cgcaacgatg acgttcggat tcagccgatc
     2101 gaaatcccgc gtgcggcttc tctcccgcgt gacctgttcg cggcgttgca cgaagctacg
     2161 gcgagagaga gcgccgcgcc cgcggcgaac acgatcccca cgcccgggct gcccgccgaa
     2221 gagatcaccg aagagcttca ccttcgaatc gatcgctgcg cctcggaggt cgtcccagca
     2281 ggtttggatt cgattctgct cgaatcgggg acacactttc agtcgccgaa cacgcacccc
     2341 agttgggcgt cggctctcgc ggcaaccagc tcagccccac cggatgaaaa ccaggccccg
     2401 ccgaactgca cggctgcgcc gctcgaccgg gctgtacgcg acgatggggc tgtgcacggc
     2461 tacatggttg cgcggaacta ccacgcccca gcgggcggcc aacaggcagg gtgggtgcgc
     2521 cgcgcggggt tagtactgat gtgcgccgtc cttgcccttt tggggggcta tatcggcgcc
     2581 cgttttgggc aaccataacc tcggctgctg cagattccac cgctcctcga taggtggcac
     2641 cacggcttgc gcatcgtcga gccgcggcgt ctgtgtaccg actcgtttgt catgggttcg
     2701 tcagagaacc gccgtcagtc cgctgacgca ctcgtgtctc gctgacacat ggcagctggc
     2761 cttttcggcg aaaatggctg cctcctctgg cggctttggc catgagcgcg actggcacgg
     2821 ctctcgcttt acagcgatgg ccatgcccaa acgccctgca cagacagctg ttctggatcc
     2881 cgtcgaacaa ttggtcgcgc gggctcgccg catgcggtcc aagggggagg cgaagcgcag
     2941 cctgacgctg ctccgcgaag cgtgctgctt ggcggagcag gacgcacgcc tttggacgct
     3001 ctactccata cagtgctggc gtctcggtca gcggaacgag ggaaggcgtg cgcttcggca
     3061 ggcgctctgg ctgcgcgagc gggaacgcga tgaatcccgc gtccgcgtgt tgaagcagct
     3121 tttgctcgcg atggagtcag cgtgcgcccc cgacgaactc cgcgccgctt gatgacgctc
     3181 gagggaggtt caatcccgaa cgcagcctag atgcggactc gcggatacgt cggttgctac
     3241 ttcaagggcg cgtaggaggc gcactagagt tcgctttcac ccgcccaggc gcgcagtgag
     3301 ctctcgccca gagcctcggc aactcgcggc gtccacacgg cacgcgggat ataatcgcca
     3361 ccccgtccaa tggagccaga ttgcggggtt tggggttggg ataggacagc aaacctggct
     3421 caccgtcgtg aacggcgacg gggtggcatc gagaagcgtg tgtctcggct aagagagctg
     3481 caacgctctt tcgagttgag ccgacaacac catcacgatg cctcgcgagc gggcgccgtg
     3541 gggcaactgg ggcaagcgtg acggcctcag agagggctca gagtgcggct tctcggaaag
     3601 ccagccctcc ggcatcaaac gaacgaagca cgttacgtcc gcagaatggg caagcccaac
     3661 ccacgtcgaa acgcggccgg ctcgcgccga gagtattcgc gggtgccaac tcctcccggg
     3721 cacatctggg gtcgcgagcg aagccgcgaa ttgccacgcg gttctcatcg agcactgcgc
     3781 ctagacgggc ttggcattca caggaggagt aaacgacggc ggtcttcatt ctgagctcga
     3841 gtagagaggc taggtccgta tcgctcggct gttgggcgtc gcgaaagccg gccaaccaga
     3901 aaggttctag ctacaggcct ggcaggcatc aggccggagg catgtaggca taatcgaggg
     3961 gccggcgcat cgtcaaggcg agcacacgtc agggcgagcc gacccgccgg gaagaacgcc
     4021 ttggcgcgtt cgcccgccat gactcaccga caattcgcta ccggtgggcc agcatgcggc
     4081 tcatccggtt cgtagagcac ggagtgagtc cctccgccaa gcgcccaaaa aaaacagagc
     4141 tcagccgtaa cacttctgtc gatttcagcc ttccctgtta acgctgagcg tgctactcga
     4201 atgcctcgcc aagcgggact agctcagttg gtagagcacg agcttcccaa gctcggggtc
     4261 acgggttcga gccccgtgtc ccgctcaggt ttcgccccat ttaggggtgt tcgcgcgtcg
     4321 agtttgcgcg cgccgtaagg tgtttgcgcg cccatacgag cgacggccgt ggctgagatt
     4381 cagcggggat tccaccagca ctcgcccttg ccacgcattg cgagttcggc gcggctgcga
     4441 atctcctgca acgcgctcgg gttcaatggc tcgaagtccc gggccgcgcg caaggccgcg
     4501 tcttgttcgt tcggaaagct catgccgagg agcgcgacgt ccggatcgag cgtcagggtg
     4561 tagcgaacgc attcgtcgac gctgaggcgc gggagctccg tttcctgaga aaccacctgg
     4621 ccccctgaac tcagcttgcc ccggggacga gggctcagcg gccgtccgta cccttc
//
LOCUS       DASTKU010000831         3998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1950914, whole genome shotgun sequence.
ACCESSION   DASTKU010000831 DASTKU010000000
VERSION     DASTKU010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950914"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFQ61_29080"
     CDS             complement(<1..346)
                     /locus_tag="VFQ61_29080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958596.1"
                     /translation="MQRSALQSSEKNSGTFAISAHSPGAADHSPSEGSTLPSEDCEAN
                     LLTGLLADREQAWREFNSRYSRLILSCIGRVTTRFGYSSQDDVREIYATLCLQLLSHD
                     KRKLRSFEPGRGT"
     gene            702..971
                     /gene="rpsO"
                     /locus_tag="VFQ61_29085"
     CDS             702..971
                     /gene="rpsO"
                     /locus_tag="VFQ61_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET9958597.1"
                     /translation="MPLQPEAKQGVISQFRLHESDTGSPEVQIALLSQRIDYLTEHFK
                     VHRKDHHSRRGLLKLVGQRRRLLDYLKRSDVERYRKVVGSLNLRR"
     gene            1183..3486
                     /gene="pnp"
                     /locus_tag="VFQ61_29090"
     CDS             1183..3486
                     /gene="pnp"
                     /locus_tag="VFQ61_29090"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET9958598.1"
                     /translation="MFVRETIVVGGRPFTFETGRMAKLAHGAVLVSYGESVVLVTAVS
                     NDERPGIDFFPLTCEYVEKTYAAGKIPGGFFKREGRLRDFEVLASRLMDRPLRPLFPE
                     GFRKDTQVIATVLSSDKENPTDVLAMSAASAALHISDIPWEGPIAGIRVARIDGEFVA
                     FPTFDQSAEADIELVVAVSKDAIVMVEGGADEASESDLIDALMFAHREAQPIIELITR
                     MREAIGKPKREFVVPKLEAEVQARVAQICDEPLRTACVIREKRARYETYSKIKADLAA
                     KLGAELGAERFTALEKLIKEEFENRKAVVVRNMVVNEGRRIDGRDTKTIRPITCEVSL
                     LPRVHGSALFQRGETQAIVTTTLGTSSDEQKIDALTGESWKRFMLHYNFPPFSTGETK
                     PMRGPGRREVGHGALAERAVSRMIPGHEEFPYTVRIVSEILESNGSSSMASVCGATLS
                     LMDCGVPIRRPVAGIAMGLIQENGKVAILSDILGDEDHLGDMDFKVCGTERGVTAIQM
                     DIKIAGLDRTVLERALAQAREGRVFILGRMLETLPTARKDINRWAPRITSIKVKPDQI
                     RLIIGPGGKTIKGIVDQTGCTIDVEDDGTVNVASADSDAVKRALNIIEGLVAEPEVGQ
                     VYRGVIKRLTDFGAFVEILPNTEALLHVSEISHDRVESPGDVLREGEEVEVKVISMDR
                     EGKIRLSRRELLPLPDGEEGERAKERIARAREAGPPQRGGGGGGREGGGPRGREGGGR
                     GGGRGGGGREGGGGREGGGGRDRGPRH"
     gene            complement(3892..3968)
                     /locus_tag="VFQ61_29095"
     tRNA            complement(3892..3968)
                     /locus_tag="VFQ61_29095"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3932..3934),aa:Asp,seq:gtc)
BASE COUNT          805 a         1164 c         1315 g          714 t
ORIGIN      
        1 gagtaccgcg gcccggctcg aagctgcgca gcttgcgttt gtcgtgactg agcagttgaa
       61 ggcaaagggt cgcgtagatc tcgcgaacgt catcctgact gctgtagcca aagcgcgtgg
      121 taacgcgacc aatgcagctc aggatcagac gcgagtaacg tgaattgaac tcgcgccaag
      181 cctgttctcg atccgcgagc agaccggtga gcaggttggc ttcgcaatcc tcggacggca
      241 gcgtggagcc ttcagaggga gagtgatcgg ccgcgccggg agagtgggcc gaaatcgcga
      301 aggtgccgga attcttttca gaagactgaa gggcggagcg ttgcatcggg tcctcgaagg
      361 ggaggcgcgt caaccgcgct tggccgtcct aagagcacct ctcgtaccag ctagtaattc
      421 ttgaatatca attacttatg gagcgtaaca gggcagggat acggtgacgc gtgacgtccg
      481 ccgtgacgct tcgaagggtt ctacgcgcca agggcagggc ctctccccaa atcagttccc
      541 aaatcgagtt ttgggtgcgt gtcgcatatc gccggccttg acacggtgcg ggggactgag
      601 cgacggaagg ccccgtcggc agccggctcc ggttgaccga tggatcgcgc ggcgtatact
      661 cgccgcccca aaaacgtatt caggataagg aattctcgca aatgcccctc cagcccgaag
      721 ccaaacaggg cgtaatctcc cagttccgtt tgcatgagag cgacaccggt tcccccgagg
      781 ttcagatcgc gctcctcagc cagcgcatcg attatttgac ggagcacttc aaggtgcacc
      841 gcaaagatca tcattcccgg cgcgggctgc tcaagttggt gggtcaacgc cgccgcttgt
      901 tggactacct caagcgctcg gatgtagagc gttaccgcaa ggtcgtgggt tctctgaacc
      961 tgcgccgctg agttcgttgg gaggctgtgc tctctacgag cacagctcac gagctgagta
     1021 ttcgtccgat actccgaggt gtggcgccta acacttcaac ttcgcttcgt ggggccgagt
     1081 ggccctcagc cctaggaatc gaggctgaag ccctaggtgc acacgttcgg cgtcacaccg
     1141 cacgtagcgg tatcgttgcg cctgaaggag cgcccctaga gaatgttcgt tagagaaacc
     1201 atcgtcgtgg gtggtcgtcc attcacgttc gaaaccggcc gcatggcaaa actcgcacac
     1261 ggtgcggtgt tggtgtcata cggcgaaagt gtcgtgctcg tcactgccgt gtccaatgac
     1321 gagcgtccag gcatcgactt cttcccgttg acttgcgagt acgtcgaaaa gacgtacgcc
     1381 gcgggtaaga tcccgggagg atttttcaag cgcgaaggtc gcctcaggga cttcgaagtc
     1441 ctcgcctcgc gtctgatgga tcggcccttg cgcccgctct tccccgaggg cttccgcaag
     1501 gacacgcaag tcattgcgac cgtgctttcc tccgataagg agaacccaac ggacgtgctg
     1561 gccatgagtg ccgccagcgc ggccttacac atttcggaca tcccatggga aggtccgatc
     1621 gcgggcatcc gcgtggcgcg catcgacgga gagttcgtcg cgttcccgac gttcgatcag
     1681 agcgccgagg ccgacatcga gctcgtggtc gcggtgagca aagacgcgat cgtgatggtc
     1741 gaagggggag ccgacgaggc ttccgagagc gatctgatcg acgcgctgat gttcgcccac
     1801 cgcgaagctc agcccatcat cgagctgatc acgcgtatgc gtgaggccat cggcaagccg
     1861 aagcgcgaat tcgtcgtgcc aaagctcgag gccgaggtgc aggcgcgcgt ggcgcagatt
     1921 tgcgacgagc cactgcgcac cgcgtgcgtg atccgcgaga agcgggcccg ctacgaaacg
     1981 tactccaaga tcaaggctga cttggcggcg aagctcggcg cagagctcgg agcagagcgc
     2041 ttcacggccc tcgagaagct gatcaaagag gagttcgaga accgcaaggc ggtggtggtg
     2101 cggaacatgg tggtcaacga aggccgccgc atcgacggac gtgacaccaa gaccatccgc
     2161 ccgatcacgt gcgaggtcag tctactgccc cgggtccatg ggtcggcgtt gttccaacgc
     2221 ggtgaaacgc aagccatcgt caccacgacg ctcggtacct cgagcgatga gcagaagatc
     2281 gacgcgctca cgggcgagag ctggaagcgc ttcatgcttc actacaactt cccgcctttc
     2341 tcgaccggtg aaaccaagcc catgcgcgga ccggggcgac gtgaagtggg tcacggtgcc
     2401 ctggcggaac gcgcggtttc tcgcatgatc cccggtcacg aggagttccc gtacacggta
     2461 cgcatcgtga gcgagatcct cgagtccaac gggtcgagct cgatggcgag cgtatgcggc
     2521 gccacgttga gcttgatgga ttgcggtgtg ccgattcgcc gacccgtcgc gggtatcgcg
     2581 atgggcttga ttcaggagaa cggcaaggtc gcgatcctga gcgacattct gggcgacgag
     2641 gatcaccttg gcgacatgga cttcaaggtc tgtggcaccg agcggggtgt gacggcaatc
     2701 cagatggaca tcaagatcgc cggcttggac cgcaccgtgt tggagcgcgc gctcgctcag
     2761 gcgcgcgaag ggcgcgtgtt catcctaggg cgcatgctgg agacgttgcc cacggcgcgt
     2821 aaggacatca atcgatgggc gccgcgaatt accagcatca aggtgaagcc ggaccagatt
     2881 cgcctcatca tcggtcccgg tggcaagacc atcaagggca tcgtggacca aaccggttgc
     2941 acgatcgatg tagaggatga tggaacggtc aacgttgcca gcgctgattc agacgcggtc
     3001 aagcgtgctc tgaacatcat cgaagggctc gtcgcggagc ccgaggtggg tcaggtatat
     3061 cgtggcgtga tcaagcgcct caccgacttc ggggcgttcg tcgagattct gccgaacacc
     3121 gaggccttgc tgcacgtgtc tgaaatctct cacgatcgcg tcgagtcccc tggggacgtg
     3181 ctgcgcgaag gtgaagaggt cgaagtcaaa gtgatcagca tggatcgcga aggcaagatc
     3241 cgcctcagtc gccgcgagct ccttccgctg ccggacggcg aggaagggga gcgagccaaa
     3301 gaacggatcg ccagagcgcg agaagctggg cccccgcaac gaggcggcgg cggtggtggt
     3361 cgcgagggcg gtggaccgcg cggtcgcgaa ggcggcggtc gcggcggtgg tcgcggcggc
     3421 ggtggtcgcg agggcggtgg aggtcgtgaa ggcggcggcg gccgtgatcg cgggccgcgc
     3481 cactgaaccc gagcttttgg acaaaggcag cccggtaagc atgtcgagcg ccactgaggt
     3541 ggcgctcgag tctgaatagc aaactcaggg tcaaccgcag agagcacaag ccgaaagagc
     3601 cgaaccccaa tggggtcggc tctttgcggt tggtttcacc gtcaaatcgt cgggcagctg
     3661 ctcgagatca tgcgcagcga actacgggga tcgcggcatc gcaaccgcca cggctccacc
     3721 gcgagagatt cagtggagta gggattgctg cacagcgaat ccgctgttgc cgagccgtcc
     3781 gtcggttcgc atcgttagcg acgacgctgc ctcggagaac gcactctcta gcgagaagcg
     3841 cgcgttctcc gacgcaggat cactaacgag caacaaacgg gaaccgcgaa ctggcggggc
     3901 ggacgggact cgaacccgcg gcctccggcg tgacaggccg gcgttataac caacttaact
     3961 accgccccgt ttgtatttct gctcgtattt ctgcagct
//
LOCUS       DASTKU010000832        10829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_385709, whole genome shotgun sequence.
ACCESSION   DASTKU010000832 DASTKU010000000
VERSION     DASTKU010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385709"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VFQ61_29100"
     CDS             <1..642
                     /locus_tag="VFQ61_29100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958599.1"
                     /translation="QSLEKPLGRTELGSTLLFVRAQQEEAAHQLERARGTYEALVERE
                     PYPVGPYWDEALLALSRLAERAKQFAVAASHLERLLSAREQSWITGSYERPAFAEARF
                     HLAELWRDHLGSPERAYREFMLVYQEHTTSRLRDDAVFQAARIALRKHDQAAACRAAR
                     VLTKDLSDSRYARCAGVICPSAISQHGVCPAYIEAELSNPSTLDANQHTRERP"
     gene            complement(890..1276)
                     /locus_tag="VFQ61_29105"
     CDS             complement(890..1276)
                     /locus_tag="VFQ61_29105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958600.1"
                     /translation="MEIHLDRRAVRQIRLSAQEAIEEDDSESLREDVLEAFTEDQVEE
                     IERRLDNGDFFEFLTDVLDEWSGDDVDELFELLETQLGEVGIDLKTEAKADDDDEEEE
                     DGDEDADFDDDDLEEDDDEDDEDDDE"
     gene            1353..2270
                     /locus_tag="VFQ61_29110"
     CDS             1353..2270
                     /locus_tag="VFQ61_29110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958601.1"
                     /translation="MRVLDIFSLLVVIFVSLEIREADAQALRRPGAPNGAPLQPDVAP
                     SSNPPEAGTPVSPANPPSTVAPPQDAATAPDAPAAADDPVVAPEAAGQTNRAAEDTAA
                     EDTAAEDNGVVAPRTPGPPPNSLDYFQSGVALTSESLGSAADVCPESAVTPCILGPGG
                     GLTIRAGYRSRGPWYVGGAYEFARLDSSNLLKLAILQQVRAEARVYKDFGTRLVPYLN
                     AGLGGVLYGSEWGASTGGMVVSAGLGLEFQITQSTVVGFSPMYRALVLRSFRDSAGQQ
                     RAKEFFGFGLAHFVAIELVFEVREPLPRW"
     gene            2287..3810
                     /locus_tag="VFQ61_29115"
     CDS             2287..3810
                     /locus_tag="VFQ61_29115"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9958602.1"
                     /translation="MNTGKGTDIPVRCDRCGRDNPAGLRFCRDCGNRLAAVSTRLPDP
                     PPPRAADPSAVRPGGANAGFAAAPGNPAVGSRAPAGVECSACHANNPPGSRYCFNCGA
                     TLASPGPSVVQPPRPAMPSASNPPRVPPGAAFGSARPGDGGLANKHVVPGRPPMPSQP
                     PPADLNASAPGMAGNAAPGNANVPVNAGALPNAGALPNAGALPNATVQCGRCRNINSR
                     DLKFCQYCGARLGSEEVITPEARPGAPGPAQPVGVPAGATVQPGAGAPPGVTAVQAPI
                     WGRLVVIAQDGTTGREYPLEEEQTDIGREEGGLLLPSDPYVSPRHARVVQRDGRFFVR
                     DLGSVNGVYIRLRSPERLQNADLVLIGLEVLRFEVVSDAEKGLGPANERGTRVFGSPA
                     SPRYARLLQRTVEGTTRDIFYLTREETVVGRETGDLVFTNDPFMSRRHALFQRDPSTG
                     TFLLQDLGSSNGTYLAIRGEYELSHGDHMRVGQHLFRLDVEPGRYEASRPEIGRART"
     gene            3807..4955
                     /locus_tag="VFQ61_29120"
     CDS             3807..4955
                     /locus_tag="VFQ61_29120"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET9958603.1"
                     /translation="MTTASTPGSIRIRLFGRTDVGQIREHNEDNFLIADLTRRSRSLM
                     ETDREQIVGERGTAIGVCDGMGGAAAGEVASQLAVDIIYEKLVNGEPLKDHNELARRL
                     VHAVEEAGVRIFNEARADRSRRGMGTTATIAALLDSRLFIAQVGDSRAYVMRNGKLVQ
                     VSRDQSLVNQLIEAGQLTEEEAETFEHNNIILQALGTAETVQVDLTYVDLRRGDLVLL
                     CSDGLSGMIRGEEIRDVLRTIPDPLEACRELTERANRAGGHDNITVIVAQFDGPGLEE
                     PSSDEEPSYQKYPLPELPAQENTLRIAPMQMPDVPYHVNPHEPRMRVGHTIMSMPAVE
                     VPGVSAGRYNSMDDPVELPTTGFPPSLVGFMVLGAMVLAAVAGFLLLR"
     gene            4959..7172
                     /locus_tag="VFQ61_29125"
     CDS             4959..7172
                     /locus_tag="VFQ61_29125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9958604.1"
                     /translation="MPVRRALGRLLAWFATLAVTSLLALWALSALATPRPSEPSLPLF
                     FNARPRNVYDLARSAIDQIVRGERSEEAARELARLGGAGLPHVLPDLDTLPPRVRGRV
                     AFALNPIAERMGVARKAELATEEDAVVFWTRFWQDRSFDFRPQVSRRLVQRLARQSSS
                     LREEDLAHLDTYCLVDVMQALGTVRSPEDVARAARLTEVLAHVTSQGQVVEPWMSVEQ
                     ANGVVRRWRRFWADHGADFLTLSGPLRVAAMFSQTRYGRFVGRVVRAAKSESEVDLIS
                     ERAGPHPITKSLLRLIAALAASVLISMLWVRLESGASGARRAGLRGAACVVLALPPWF
                     IATGLGLGGPRFHPAELGPMVLARELGLLALGALLGGAILSRCMLGALSHAGDPREVV
                     WRELSGVSLRALPSALPWLLGSQFCLEIAGDSTGLAKATLDALGRGQVTTGILVALGT
                     TALSWLLLVLASWAPLLGGRTARPPALLEVDGPSRRRLLLASAALLGLLLALAGAGHT
                     DVSRGLGLIEVCHGAEALLMLSVSTLVIAVVLGIAAGAAAASGPRLFDSLLLRSTEIA
                     SALPAFMWAAALALSLQSGFAFACALGVLRAIDIAWLLRSELVELAELDSELGLRSLG
                     RLPIRAYVRERLRPAVLSALAGVALTPAWALGIAAAVRFLDLPPLSSRRAWDDLLSGA
                     HAVMPGAWLFAAALLSLVSFLSLTAVASPSRRVGSLRSNPPPKLVSSPPPAGGAA"
     gene            7256..7897
                     /gene="trxA"
                     /locus_tag="VFQ61_29130"
     CDS             7256..7897
                     /gene="trxA"
                     /locus_tag="VFQ61_29130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET9958605.1"
                     /translation="MALTTVTERDFEQQVMMSELPVLVEFSAEWCGPCKVIAPELDAL
                     SKELSGKAKLVKIDIDKSPMLAQQMGIKSVPTFVVFHNGRPVGGRVGALKRPQLREML
                     DPFLPRAEGALKPEEVVQLLKQGRISLVDVREPAVYQRARIAGAVNIPHSEIETRLAE
                     LHMLPNHPVMYCRSGEQTKELSAKLAEQGMNIAFIEGGVLAWEAAGLPLDRPD"
     gene            complement(7956..9140)
                     /gene="larC"
                     /locus_tag="VFQ61_29135"
     CDS             complement(7956..9140)
                     /gene="larC"
                     /locus_tag="VFQ61_29135"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HET9958606.1"
                     /translation="MLFLDTPSGIAGDMTVAALIDLGVPLAVVTDAISRLELEEVRVS
                     ASPARSGVVGGTRFHVEVTGAHPERRYAEIVELLDRSSLETRTRSLARRVFHHLAEAE
                     SEVHRMPLDDVTFHEVGAVDAIVDIVGACAAFDFLRARVLATPLPMSRGFVRCEHGWL
                     PLPAPATLLCLRGVPTYPSGLDVELVTPTGAAILASVAEGFVAWPKLTPECVGFGCGT
                     RRLADGRPNALRAVLGTEPSTAAVESAPASSSHVVLSANVDDMTGELAGYVIERLFHA
                     GALDAWAEPVTMKKGRPGLILCALGQGTDAAALAGVILRETNSIGVRSSSVSRFERPR
                     RVLQVDTRFGTISVKVSEGDYGSPLAKPEFEDCARAAREYGVPLRDVLTEAQLAFAKL
                     AR"
     gene            complement(9371..10153)
                     /gene="larB"
                     /locus_tag="VFQ61_29140"
     CDS             complement(9371..10153)
                     /gene="larB"
                     /locus_tag="VFQ61_29140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HET9958607.1"
                     /translation="MNPSQLQELLQRVQRGELAVNDAQRALEHLPFSELEYAKVDHHR
                     ALRLGLPEVIFGEGKRAEQIVGIAQRLVAHQQNVLVTRVAPALAEAVLNSFPELKYAE
                     LARTLSYEASPITPFACEPVAIVTAGTSDLPVAEEALETLRMCGVPASRVFDVGVAGI
                     HRLFSRLETLTRAPVVIVVAGMEGALPSVVGGLVGAPIIAVPTSVGYGTALSGFTALF
                     GMLTSCASGLTVVNIDNGFGAAVAALRMVRHGLGRTSAGPTP"
     gene            10572..>10829
                     /locus_tag="VFQ61_29145"
     CDS             10572..>10829
                     /locus_tag="VFQ61_29145"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9958608.1"
                     /translation="MDRIDALLDQVVEVCPLPATTQRVLALTASDKSKVAEVASVIST
                     DPALAAAVLSVANSAAYAANKVDSLDMAVMRLGLRELNNLAA"
BASE COUNT         1876 a         3513 c         3478 g         1962 t
ORIGIN      
        1 cagtcgctcg agaagcccct cggtcgaacc gagcttggct ccactctgct gttcgtccgc
       61 gcccagcaag aagaggcggc gcaccagttg gagcgagcgc gtggtaccta cgaggcgctg
      121 gtcgaacgcg agccataccc ggtggggccg tactgggatg aagccctatt ggcgctttcg
      181 cggttggccg aacgagcaaa gcaattcgca gtagcagcct ctcacctcga gcgactgctt
      241 tctgcgcgcg aacagtcttg gatcaccggc agctatgaac ggcctgcctt cgccgaggcc
      301 cgcttccatc tggcggagct atggcgcgac catctgggca gccctgagcg ggcctaccga
      361 gagttcatgc tggtttacca ggagcacacg accagtcggc tgagggacga cgccgtattc
      421 caagcggcgc gcatcgcgct gagaaagcac gaccaagccg ccgcatgccg cgcagcccgt
      481 gttctcacca aggatttgtc cgactcgcga tatgcgcgtt gtgccggcgt gatatgcccc
      541 agcgcaattt cgcagcatgg cgtctgcccg gcctatatcg aagccgagct ctcgaacccg
      601 agcacactcg acgcaaatca gcacactcga gaaaggccct gaccgctcgc gcggacaaag
      661 cccctcgttc ggctgttgcc gtcgaacagc gcgcgtttta tcgaacgcga tccgtcgtcg
      721 aacgcagccc gcttcgaacg caagcgttat cgaatatacg gcacatcgta cgcaatgccc
      781 tatccctgag cactgcgtac acggacgctg gaagtcgaac ggcgacgccg gagaaacgac
      841 tcgtactctg gcggaacttc gggacgaatc ccgaaagtcc gcctagagat cactcgtcgt
      901 cgtcttcgtc gtcctcgtcg tcatcctctt cgaggtcatc gtcgtcgaag tctgcgtctt
      961 cgtcgccgtc ctcttcttcc tcatcatcgt cgtcggcttt ggcttcggtc ttcagatcga
     1021 tgccgacctc acccaattgg gtttcgagca gttcgaatag ctcgtcgaca tcgtcgccgc
     1081 tccattcgtc caggacgtca gttaaaaact caaaaaagtc accattgtcg aggcgccgtt
     1141 caatctcctc aacctgatct tcagtaaacg cttcaagaac gtcttcgcgg agggactctg
     1201 aatcgtcctc ttcgatggct tcttgtgcgg acaggcgaat ctgacgaacc gcgcgtctgt
     1261 cgaggtggat ttccatgaaa gcgcttcctc tcggacccga cccggcatta aacgaaggag
     1321 cttgcccgag tcaaggccgg ttcgttcatc ctatgcgtgt tctcgacatc ttcagccttc
     1381 tcgtcgtgat tttcgtgtca ctcgagattc gcgaggcgga cgcgcaggca ctgcgacgtc
     1441 ccggtgcgcc aaacggagcc ccccttcaac cggacgttgc gccgagctcc aaccccccgg
     1501 aggcgggaac gcccgtctca ccggcgaacc cgccgagcac agtggctccc ccgcaggacg
     1561 ccgctaccgc accggacgcg ccggccgcag ctgacgaccc tgtggtcgcg ccggaggccg
     1621 ccgggcagac caacagagct gcggaagaca cggctgcgga agacacggct gcggaagaca
     1681 acggtgtggt ggcgccgaga acgcccggcc ctccgccgaa tagtctcgac tacttccaat
     1741 ccggcgtcgc gctgaccagc gagagcctcg gatcggcggc cgacgtttgc ccggaatccg
     1801 cagtcacccc gtgcatcttg ggtcccggtg gcgggctcac gatccgagca ggctatcgct
     1861 cgcgcgggcc ctggtacgtc ggtggtgcct acgagtttgc acgtctcgac tcatcgaacc
     1921 tgctcaagct cgccatcttg cagcaggtgc gcgcggaagc gcgcgtctat aaggacttcg
     1981 gcacgcggct cgtcccgtac ctcaacgcgg gcctgggtgg ggtgctgtac ggctccgagt
     2041 ggggtgcgag cacgggaggc atggtagtca gcgcggggct cggtctagag tttcagatca
     2101 cgcagtcgac cgtggtaggg ttctccccca tgtatcgggc cttggtgctg cgaagctttc
     2161 gagatagcgc tggtcagcag cgcgccaaag agttctttgg gtttggccta gcccactttg
     2221 tcgcgatcga actcgtattt gaggtgcgag aacctctgcc gcgctggtaa ggctggccta
     2281 tctgccgtga atactggcaa gggtaccgat atccccgttc gctgcgatcg ctgtggacgt
     2341 gacaaccccg cggggcttcg tttttgccgc gattgcggca ataggctcgc cgcggtctcg
     2401 acgcgcctgc ccgatccacc cccaccgcgt gcggctgatc cctctgcggt acgcccggga
     2461 ggcgccaacg ccggtttcgc cgccgcgccc ggaaatccgg cggtaggctc gcgggctccg
     2521 gctggcgtgg aatgttcggc gtgtcacgca aacaatcctc ccggcagccg ttactgtttc
     2581 aactgcggag ccactttggc ctcacctggc ccaagtgtcg tacagccccc gcgccctgcc
     2641 atgccttctg cgtccaatcc gccgagagtg ccacccggag cggcctttgg tagtgcacgt
     2701 cccggcgacg gtgggctggc aaacaagcac gtggtgccgg gtcgtcctcc aatgccgagc
     2761 cagccgccac ccgctgatct gaacgcgagt gctccgggca tggcgggaaa cgccgcgcca
     2821 ggtaatgcaa acgtgccggt gaatgcgggc gcgctgccaa atgcgggcgc gctgccaaat
     2881 gcgggcgcgc tgcccaatgc aacagtgcag tgcgggcgtt gccgcaacat caactcgcgc
     2941 gatctcaagt tctgccaata ctgcggcgct cggctcggga gcgaagaggt catcaccccg
     3001 gaagcgcgcc caggagcgcc cgggccagcg cagcccgtag gagttccggc gggagcaaca
     3061 gttcagcctg gagccggcgc tccacctggc gttacggcag tgcaagcacc gatctggggg
     3121 cgcctcgtcg tgatcgcgca agatgggacg acaggtcgag agtaccccct agaagaggaa
     3181 cagacggaca tcggccgcga agaaggtggc ttgctcttac cgagcgatcc ctatgtttct
     3241 ccccgccatg ctcgggtagt tcagcgggac ggccgctttt tcgtccggga tttgggctcg
     3301 gtgaacgggg tgtacatccg actccgaagc ccagaacgac ttcaaaacgc ggacttggtg
     3361 ttgatcggcc tcgaggtgtt aaggttcgag gtcgtgagtg atgcggagaa aggtctcggg
     3421 cccgccaacg agcgaggcac gcgcgtgttc ggttccccgg catcaccgcg ttacgcccgt
     3481 ttgctgcaac gcactgtgga aggcactacc cgcgacatct tttacctgac tcgggaagag
     3541 accgtggtgg gccgggaaac cggcgacttg gtgttcacga acgatccatt catgagccgg
     3601 cggcacgcgc ttttccaaag ggacccctcg acggggacat tcctgctgca ggatctcggt
     3661 tcgtcgaacg ggacgtatct cgcgatccgg ggtgaatacg agctctctca cggcgaccac
     3721 atgcgcgtgg gtcaacactt gtttcgactg gatgtcgaac ccggccgcta cgaggcgagc
     3781 cggccagaga tcggcagggc gcggacatga cgacggcgtc cacgcccggt agcatccgca
     3841 ttcgactctt cgggcgcacc gacgttgggc agatccgcga gcacaacgag gacaacttcc
     3901 tgatcgcgga tctgacgcgc cgcagccgca gcctgatgga gaccgatcgc gagcagatcg
     3961 tcggtgagcg cggcaccgcg attggcgtct gtgatggtat gggcggcgcg gccgccggtg
     4021 aagtcgccag ccagctcgcg gtggacatca tctacgagaa gctcgtcaac ggtgagccgc
     4081 tcaaggatca caacgagctc gcgcgtcgcc tggtccacgc ggtcgaagaa gctggcgttc
     4141 ggatcttcaa cgaggcccgc gcggaccgca gccgccgtgg catgggcacc accgccacga
     4201 tcgctgcact gctcgacagc cgtctgttca tcgctcaagt cggtgacagc cgcgcctatg
     4261 tgatgcgcaa cggcaagctc gttcaggtga gccgcgacca gtcgctcgtc aatcagctga
     4321 tcgaggcagg acagctgacg gaagaagaag cggaaacctt cgagcacaac aacatcattc
     4381 tgcaagcgct cggcacggcg gagacggtcc aagtggactt gacgtacgtc gatctgcgcc
     4441 gcggggacct ggtgttgctc tgttccgatg gtctttcggg catgatccgc ggtgaagaaa
     4501 tccgcgatgt gctgcgcacc attcccgatc cactggaggc atgtcgagag ctcaccgagc
     4561 gggccaaccg cgccggcggt cacgacaaca tcaccgtgat cgtggcgcag ttcgacggcc
     4621 ccggtttgga agaaccgagc tcagacgaag agccctcgta tcaaaagtat ccgctgcccg
     4681 agttgcccgc gcaggagaac actctgcgca tcgcacccat gcagatgccg gacgtgccgt
     4741 atcacgtcaa tccccacgag ccgcgcatgc gtgtggggca cacgattatg agcatgccgg
     4801 ccgtggaagt gcccggagtc agcgcgggtc gttacaactc gatggatgat cccgtcgagc
     4861 tgccgaccac gggcttccct ccgtccctcg ttggcttcat ggtgctcggg gccatggtgc
     4921 tggcggccgt ggccgggttc ctgttgctgc ggtgagcggt gccggttcgg cgcgctttag
     4981 gacgtctgct cgcttggttt gcgacgctcg cggtgacgtc gttgctggct ctgtgggcgc
     5041 tctccgcctt ggccacgccg cgcccgtccg agcccagcct gccgctattc ttcaacgccc
     5101 gcccacgcaa cgtttacgac ctcgcgcgct ccgcgatcga ccagatagtg cggggcgaac
     5161 gcagcgagga ggcggcacgc gaactcgcac gcttgggcgg ggcgggcctg ccgcacgtgc
     5221 tgcccgatct ggatacgctg ccgccacgcg tgcgtgggcg cgtcgctttc gctttgaatc
     5281 caatcgcgga acgcatgggc gtggcccgca aagcggaact cgcgacggaa gaagacgcgg
     5341 tcgtgttttg gacgcgtttt tggcaggatc gttcgttcga tttcagacct caagtctcgc
     5401 gccggctggt gcaaaggcta gcgcgccaat cttcttcgct gcgtgaagag gatctggcgc
     5461 acctagacac ctactgccta gtcgatgtga tgcaggcgct cggcaccgtg cgctcgcccg
     5521 aagacgtggc gcgggcagcg cggttgacgg aggtgctcgc gcacgtgacg agccaaggcc
     5581 aagtcgtcga accgtggatg agcgtcgagc aagcgaacgg cgtggtgagg cgttggcggc
     5641 gattttgggc cgatcacggc gcggacttct tgaccttgag cggccccttg cgcgtcgccg
     5701 ccatgttttc gcagacccgc tacggacgct tcgtaggccg cgtggtacgc gccgcgaaat
     5761 ccgagtccga ggtggatctg atctcggagc gggccggtcc ccatcccatc accaagagcc
     5821 tgctgcgctt gattgcagcg ctggcggcga gcgtgctgat ttcgatgctc tgggttcgcc
     5881 tggagagcgg cgcctcgggc gcgcgaagag ccgggcttcg cggcgccgcg tgcgtcgtgc
     5941 tcgctttacc tccctggttc atcgcgacgg ggctcggcct cggtgggccg cgctttcatc
     6001 ccgcggagtt gggaccgatg gtgttggcgc gagagctcgg gctgctcgcg ctgggggcat
     6061 tactcggcgg cgccatactt tcccgttgta tgctcggagc gctgagccat gcgggagacc
     6121 cacgcgaggt cgtgtggcgt gagttgtcgg gggtctcgtt gcgcgccttg ccgagcgccc
     6181 tgccctggct actcggctct cagttttgcc tcgagattgc cggggactcc acgggcttgg
     6241 ccaaagctac gctcgacgca ctcggccgag gtcaagtcac gacggggatc ctggttgcac
     6301 tcggcacgac ggctctatcg tggttgttgt tggtgctggc cagctgggca ccgctgctcg
     6361 gcggccgcac cgctcgacct ccagcgctcc tggaggtgga tgggccgagc cgtcggagac
     6421 tgctgctcgc ttccgcggcg ttgttgggcc ttctgctcgc acttgccggc gcggggcaca
     6481 cggatgtgag ccgcggcctc ggcctgatcg aagtttgcca tggcgcggaa gcgctcttga
     6541 tgctgagcgt ctcgaccttg gtcatcgccg tggtactcgg gatcgcagcc ggagcagccg
     6601 ccgcgagtgg gccccgtctg ttcgatagcc tgctgctccg cagcacggaa atcgcctcgg
     6661 ctttgcccgc tttcatgtgg gcggcggcgc tggccttgag cttacagagt ggatttgcgt
     6721 tcgcgtgcgc cctgggtgtc ctgcgcgcca tagatatcgc gtggctgctc cggagcgagt
     6781 tggtggagct ggccgagctc gactccgaat tggggcttcg ctcgttgggt cgcttgccca
     6841 ttcgagcgta cgtgcgcgaa cggttgaggc cagcggtgct ctcggccttg gccggtgtgg
     6901 cgcttacgcc ggcctgggcc ttgggcatcg cggcagcggt gcggttcctg gatttgcctc
     6961 cgctgtcctc ccggcgagcc tgggatgacc tactttcggg cgcgcacgcg gtgatgcccg
     7021 gcgcatggct gttcgccgcc gcgttgctga gcctggttag cttcttgtct ttgacggcgg
     7081 ttgcttctcc gtcacgtcga gttggctcct tgcgctccaa cccacccccc aagctcgttt
     7141 cgagtccgcc gccggccggc ggggcggcat gagaatctct ggcgatcgag caatctaggt
     7201 ggccacggag gggcttgcac ttcgcgcctg aatggtcgag atcacgagca acagcatggc
     7261 gctaacaacg gtcactgaac gcgacttcga acagcaagtc atgatgagcg agctccccgt
     7321 actcgtggag ttttctgcag agtggtgtgg cccgtgtaag gtgattgcgc cggagctaga
     7381 cgccctctcc aaggagctct cgggcaaagc caagctggtc aagatcgaca tcgacaagtc
     7441 gccgatgctc gcgcagcaaa tgggcatcaa gtctgtgccc acgttcgtgg tgttccacaa
     7501 tggtcgtccc gtcggcggcc gcgtgggtgc cttgaagcgg ccccaattgc gcgaaatgct
     7561 ggacccgttc ttgccccgcg cggaaggcgc gttgaagccc gaagaagtag tgcagctact
     7621 gaaacaaggc cgtatttcgt tggtggatgt gcgcgagccc gccgtgtatc aacgggcccg
     7681 catcgcgggc gccgtgaaca tcccccattc ggaaatcgag acgcgcctcg ccgagctgca
     7741 catgctgccc aatcatccgg tgatgtactg ccgctccgga gaacagacga aagagctctc
     7801 tgccaagctg gccgagcaag gaatgaacat cgccttcatc gagggtggag tgctggcgtg
     7861 ggaagcagca ggtctaccgc tcgatcgacc agactgaaca gtgcttccca tggaacgagc
     7921 cgtacgctga tgcgaatggc tcgttcccca agggttcacc gcgcgagttt ggcgaacgcg
     7981 agctgtgcct cggtgagcac gtctcgcagc ggcacgccgt actcgcgcgc cgcccgggcg
     8041 cagtcctcga actcgggttt ggccagtggc gagccgtagt cgccctcgct caccttcacc
     8101 gaaatcgtgc caaagcgcgt gtccacttgc agcacgcggc gtggacgctc gaagcgagaa
     8161 acagagctcg agcgcacgcc aatgctgttc gtctcgcgca gaatcacgcc cgccagcgcg
     8221 gccgcgtcgg tgccctgccc caatgcgcac aggatcaacc cgggtcggcc tttcttcatg
     8281 gtcacgggct ctgcccaagc gtcgagggcg ccggcatgga acaagcgttc gatcacgtag
     8341 cccgctagct ctcccgtcat atcgtccacg ttcgcggaca acacgacgtg tgaagagctc
     8401 gccggcgccg actcgacggc cgcggttgac ggctccgttc ccaacacagc cctgagtgcg
     8461 ttggggcgtc cgtccgccaa gcggcgcgtg ccacagccga agcccacgca ttcgggggtg
     8521 agcttgggcc aagcgacgaa gccttcggcc acgctcgcca gaatggcggc cccagtgggc
     8581 gtcacgagct cgacatccaa accagaaggg taggtcggaa cgccgcgcaa acacagcagg
     8641 gtcgctggag ctgggagcgg caaccagccg tgctcacagc gcacgaagcc tcggctcatc
     8701 ggcaacggcg tcgccagcac ccgggccctc aagaaatcga acgccgcgca agcgcccacg
     8761 atatccacga tggcgtccac cgcgcccact tcgtgaaacg tgacgtcgtc gagcggcatt
     8821 cgatgcacct cgctctccgc ctcggccaga tggtgaaaca cccggcgggc cagcgagcgg
     8881 gtccgcgtct cgagactcga gcggtccaag agttccacaa tctcggcgta gcgccgctcc
     8941 ggatgagcgc cggtaacctc gacgtgaaag cgcgttccgc ccactacgcc agagcgcgcc
     9001 gggctcgccg agactcgcac ctcttcgagc tccaaacgag agatcgcgtc cgtcacgacg
     9061 gcgagcggca ccccgagatc gatcagagcc gctaccgtca tgtcgccggc gattccgctc
     9121 ggcgtatcca agaacagcac cttgcccgca ccggcccctg gctgcagggg agcgttccgg
     9181 gatggcagtg gcggccctag gtcatgctca tgaagctcac catccccatg cgagtgcgag
     9241 tgcgagtgcg aatgcgagtg cgagtgactg tgcgaatgcg aatgcccatg cgagctggaa
     9301 ctgtggccgc tacggatggg cgcactctgt aactcccgcg ggtcgaagct ctttggctta
     9361 cgggggccgc tcatggtgtc ggtcccgctg aggttcgccc gagaccgtgg cgcaccatcc
     9421 gcaacgcagc caccgcagcc ccaaagccgt tgtcgatatt caccaccgtc aagccagacg
     9481 cgcaactggt cagcatcccg aacaacgccg tgaaaccgga aagcgcggtg ccataaccga
     9541 ccgaggtcgg aaccgcgatg atcggtgcgc ccaccaagcc gccgaccacg ctgggaagag
     9601 cgccttccat gcccgcgacc acgattacga ccggagctcg cgtcagcgtc tccaaccgac
     9661 tgaaaaggcg atgaatgccg gccacaccca cgtcgaacac gcgggacgcc ggcacgccgc
     9721 acatgcgcag agtctccaac gcttcttccg ctaccggtaa gtcgctggtc ccggccgtta
     9781 cgatggccac tggctcacat gcgaacggcg ttatcgggct ggcttcgtag ctgagcgtgc
     9841 gcgcgagttc ggcgtatttc agttccggga aactgttcag aaccgcctcc gccagtgcgg
     9901 gcgcgacccg cgttacgagc acgttttgct ggtgagccac caagcgttgc gcgatgccca
     9961 cgatctgttc ggcgcgcttg ccctcgccga agatcacctc tggtaggccc aacctcaatg
    10021 cgcggtggtg atcgaccttg gcgtattcga gttcgctgaa tggaagatgc tcgagcgcgc
    10081 gctgcgcgtc gttcaccgcg agctccccgc gttgaacgcg ctgcaacaat tcctgaagtt
    10141 gactcggatt catgcgcact cagctagccg ggttgggcag gattcgcgaa cgtccgcctc
    10201 gcgcgaggcc ggtggtcgcg ggaggcatct atgcgaggca gaaagtggtg gtcgagcaga
    10261 acccgagcca acgaacgtca ggttcctcag ggtggggaaa gcggggtcga gtgccgtatg
    10321 cgggcgcgac tcgcgcttga cccgaagcgc cgagcgggcg ctggcaacgc cgcaatttcc
    10381 gcgaagcatg acggcggttt agctccgttc ctgcgacgtg tcgactggtc gaagcactct
    10441 gccgacaccc gttcccgcag tgtaccccga tgtcgcaggg gccaccgccg cagaccaact
    10501 gcgcggaaac tcgcagcccc cgagcattcg cctcgaccac ggcgccggag ggacgctagt
    10561 ctgaagcatc aatggatcgg attgacgcac tactcgatca agtcgtcgag gtttgcccct
    10621 taccggccac cacccagcgc gtgctggccc tgaccgcgag cgacaagtcg aaagtcgccg
    10681 aagtcgccag cgtgatatcg acggacccgg ccctggccgc cgccgtactt agcgttgcca
    10741 atagtgccgc gtatgccgcg aacaaagtcg attccctgga catggccgtc atgaggctgg
    10801 ggctccgcga gctgaacaac ctggccgcc
//
LOCUS       DASTKU010000833         5724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_385715, whole genome shotgun sequence.
ACCESSION   DASTKU010000833 DASTKU010000000
VERSION     DASTKU010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385715"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VFQ61_29150"
     CDS             <1..617
                     /locus_tag="VFQ61_29150"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HET9958609.1"
                     /translation="CALITSASLVYFDWGTLPPFVVEKLPLRFESLWLASLRVHVTSA
                     LLSFPLCLLLMTRALQKRPRIHRVLGRSAGLLVLLALVPSGAVLAFDAKGGAPVTAGF
                     LLSGAIVGGCTVYGVIAARRRDLVAHRRAMRHVVAQMSVAVSSRAFIIVLDLLGTDPD
                     LAYVVALWAPVLVSALIAELPWIRFNRIRDFGWPLIRTIQKESL"
     gene            812..1039
                     /locus_tag="VFQ61_29155"
     CDS             812..1039
                     /locus_tag="VFQ61_29155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958610.1"
                     /translation="MQTTTSRDKKGREFVPFSIEVRYGNEWREDIAGCVYKSGELFVK
                     MGDEYRPAAILFGKAVEPVAGVCEAAPPARS"
     gene            complement(1062..2291)
                     /locus_tag="VFQ61_29160"
     CDS             complement(1062..2291)
                     /locus_tag="VFQ61_29160"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET9958611.1"
                     /translation="MSQPDSPHSAQLEARLLTTLEELLQQPALRLREALTAAAHRLAE
                     VLGCEKVDAFIFDESKQSLVALGTSRTPMGVRQQALGLDILPLANGGWAVHTFRTGKA
                     HLVGNAEEDGEELTGIVRDLGVRSEINAPLLVSDRRRGVLSAVSAKVNFFTPDDLRFL
                     QVVSGWVGSLVQRAELIDQIRETESKNARRAAADELVMVLAHDLKNHIHPLLGRLHLL
                     KLKLEAGQGTSAQDLDAALRSAERMSRLTSEMLDVARLDQGLFSLRLETVDVAELARD
                     VGGYCGTTRVGIEVKAPEELKLIGDPDRLRQALENVVANAVKHSPVGVPVRLDVSPDP
                     EDVSYVRIVVADSGSGISPELLPRLFERFSSGGAAAGLGLGLHLAREIARAHHGSLTL
                     DPHPGEGARFCFRLPRG"
     gene            complement(2606..3850)
                     /gene="murE"
                     /locus_tag="VFQ61_29165"
     CDS             complement(2606..3850)
                     /gene="murE"
                     /locus_tag="VFQ61_29165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HET9958612.1"
                     /translation="MTGRLAPASAPYAPWASGLHSIGVTGTNGKTSTTLFVAEVLRAA
                     GLAVASETTIGVGARLGEYDSPSQSTFFDRMRGHADAGVTHLALEVTSEALASGWAKR
                     WRFDAGVFTNLSHDHLNAHGSFEHYLASKAQLFVHLPSGAVAVFNSADPVSGWLERVT
                     PADVRRLYFAAASRGPAMRAADLAARLTHVGPLGTTAELEPSELSEALDRKLEIELLG
                     EVFLENALAAALVAWGHGIEPQTIQRGLRSVSKIPGRFEVIARAPWVVVDYAHTPDAL
                     ARTLATGRALLADTSGAKLIVVFGAGGDRDANKREAMGVAVGRRADVAILTNDNPRRE
                     DPARIAEALARGCAQAGCARVVIELDRARAIDRALNSAGSRDLVLICGKGHERTQTER
                     GITRHFDDAALVRAWLGMTSSA"
     gene            complement(3911..4936)
                     /locus_tag="VFQ61_29170"
     CDS             complement(3911..4936)
                     /locus_tag="VFQ61_29170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958613.1"
                     /translation="MGLSFERFLQITCLLAVGSSAAPGCKVEVTDGEDRESRSGGSSS
                     AQGGSSGQKTNSGGAGEGGGGSGGTLENQGGSGGEDTVNAGAGGADADSKGGTDSGSG
                     GSDSMADAGAGGQGVSSCVSGAPAEEGLGFDCASLPFAEAMCPNPTGEGLEVPVVGMQ
                     LCEYYANQRTGSVQVLTECLAKLGSDGSACTLAAAEAAYDCEAKMLAQTCASPRAEAL
                     CDQLQVNCAEIPQSQCIRDLSPLSDEKIADIENCLDGDEAVSCEYSYRACRGIPERAV
                     TREAACDSLMAQCAGIERRTCLLRVDVYGTGVLWETTLELYRECTVRSTGTGGGDCGQ
                     AFLSCTD"
     gene            5278..>5724
                     /locus_tag="VFQ61_29175"
     CDS             5278..>5724
                     /locus_tag="VFQ61_29175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958614.1"
                     /translation="MRTRVLIWGGLGCLLLSCSQDYAGGEANDRSRAAGASIGTAASG
                     GRGGTRSGVRVGPTHSHSGGAVNRNSSAPNSSDVTGSSESDGRAAGGRSGSDVSEERG
                     TVQSETLESDDIGCGGGGGGGSGGDCGVSEPPVAAAGGGWGWGDGMG"
BASE COUNT         1024 a         1886 c         1761 g         1053 t
ORIGIN      
        1 gttgcgccct gatcacgagc gcaagtctgg tctacttcga ttggggaacg ctgccgccct
       61 tcgtggtcga gaagcttcct cttcgcttcg aatccttgtg gttggcctca cttcgggttc
      121 acgtcacgag cgcgctgctg agctttccct tgtgcctttt gctaatgact cgcgcgctgc
      181 aaaagcggcc gcggattcat cgcgtcttgg gcaggagcgc gggccttcta gtgctcttgg
      241 cgctcgtgcc ttcgggggcg gtgctcgcgt tcgatgccaa gggtggagct ccggtcacgg
      301 ccggattcct gctctcgggt gcgatcgttg gggggtgcac cgtgtacggc gtgatcgcgg
      361 cgcggcggag ggacttggtc gcgcaccggc gagcgatgcg ccacgtggtc gcccaaatga
      421 gcgttgccgt gagctcacgg gcgttcatca tcgtgttgga cctattaggg accgatcccg
      481 acctcgcgta tgtcgttgcc ctttgggcgc ccgtgctcgt cagcgccctg atcgcagagc
      541 tgccttggat ccgattcaac cggattcgcg actttggttg gcctctgatt cgaaccattc
      601 aaaaggaatc gctgtgaaat tccaccgctc gctcttctgg tacgcggccg tgctaacggt
      661 cgttccgtct cttgcctggg ccgcagacgc ctcccccgtc gagtgggcaa ggcgccgtgt
      721 tcaggacggg atcgtccggc ccctcaacga acaaaagccg agccgctttt cgcgctcacg
      781 ccccgtcccg cgtgagcggc gagccagcat cgtgcaaacc actaccagcc gcgacaagaa
      841 gggtcgagag ttcgtgccgt tctcgattga agtgcgctac ggcaacgaat ggcgcgaaga
      901 tatcgcaggc tgcgtctaca agagcggtga gctcttcgtc aaaatggggg atgaatatcg
      961 gcccgccgcc attcttttcg gcaaagccgt agagcccgtc gccggcgtgt gtgaagccgc
     1021 accgccagca cgatcctagt ctcattgcgt gctcggttgc attagccgcg gggcagtcga
     1081 aagcagaaac gggccccttc gcccgggtgg ggatccagag tcaaactgcc gtggtgagct
     1141 ctagcgatct cgcgcgcaag atgtagccca agccccagtc ccgccgcggc tcccccggag
     1201 gaaaaacgtt cgaagagccg cgggagcaac tccggactta tcccggaccc gctgtccgcc
     1261 accacgatcc gaacgtaact cacgtcctcc ggatcgggcg agacgtccag gcgcaccggc
     1321 acgcccaccg gtgaatgctt caccgcgttc gcgaccacgt tctcgagagc ctggcgcaag
     1381 cgatcgggat cgccgatcag tttcagctct tcaggggcct tgacttcgat tcccacgcgg
     1441 gtcgtgccgc aatagccccc gacgtcccgt gcgagctccg ccacgtccac cgtctcgagt
     1501 ctgagcgaga agagcccctg gtccaggcgc gccacgtcca gcatttcgga cgtgagccga
     1561 gacattcgct ccgccgaacg aagcgccgcg tccaagtcct gggcactcgt gccttggccc
     1621 gcttcgagct tgagcttcaa caagtgcagc cgccccaaca acgggtgaat gtggtttttc
     1681 aagtcgtgcg ccagcaccat caccagctcg tccgccgccg cacgtcgcgc gtttttcgat
     1741 tcggtctctc gaatctgatc aatcaactcc gcgcgctgca cgagggagcc gacccacccc
     1801 gaaacaactt gtaaaaatcg caggtcgtcg ggcgtaaaga agttcacttt cgcggagacg
     1861 gcgctgagca cgccgcgccg tcgatcactc accagcagag gcgcattgat ctccgagcga
     1921 acacccagat cgcgaacaat ccccgtgagc tcttccccat cctcctcggc gttcccaacc
     1981 aagtgcgctt tgccggtgcg gaatgtgtga accgcccaac cgccgttggc gagcggcaaa
     2041 atgtcgagcc cgagcgcctg ctgccgcacg cccatcggcg tgcggctcgt gcccagagcg
     2101 accagcgatt gcttcgactc atcgaagatg aaggcgtcca ctttctcgca cccgagcacc
     2161 tccgccagcc ggtgtgccgc cgcagtcagc gcttccctca agcgcagcgc cggctgctgc
     2221 agtagctcct cgagcgtggt caggagacgc gcttcgagtt gcgcggagtg gggcgaatca
     2281 ggctgcgaca tatcctcctc atgaggcatt cgggttctcc cgacgtacgg attgcgaccg
     2341 ctttgccgcg cctttttgca tctatcgata tctctccctc gggtcgaatc caaatctgca
     2401 cggtatgtgc cagcgtcata tcaccaagtt ttgtgtggcc gcacctttgt aaagagcgcg
     2461 ccgccagtca cagtttcccc tagcgcagtc agctgtaacc ccgacagcac ctcccaccgc
     2521 gcaggcgccc acctcgccca cggaatccgc agagcgcgac cacgggatac agaattcccg
     2581 taatgccctt gcccgacgag ggatctcagg cgctcgaggt cataccaagc caagcacgca
     2641 ccagcgccgc gtcgtcgaag tggcgagtta tcccgcgctc ggtctgcgtg cgctcgtggc
     2701 cttttccgca aatcaggacc agatcgcgtg aaccagcgct gttcaatgcg cgatcgatgg
     2761 cgcgagcccg atccagctcg atgacgaccc gtgcgcaccc cgcctgagcg caaccccttg
     2821 cgagcgcttc ggcgattcga gccggatctt cgcggcgcgg gttgtcgttc gtcagaatgg
     2881 cgacatcggc gcggcgcccg accgcaacgc ccatagcctc tcgcttgttg gcgtcgcgat
     2941 cgccgcccgc tccgaacact acgatcagct tggcgcctga cgtgtccgcg agtagggccc
     3001 gtcccgtagc caaggtgcgc gcgagcgcat cgggcgtgtg cgcgtagtcc acgacgaccc
     3061 aaggtgcgcg cgcaatcacc tcgaaacgcc ctgggatttt gctcacactc cgtagaccgc
     3121 gttggatggt ctgcggttcg attccgtgcc cccaggccac cagcgcagcg gccaaagcgt
     3181 tctcgagaaa cacctcaccc aagagttcaa tctcgagctt gcgatctaga gcctcggaga
     3241 gctcggaggg ctcgagctcc gccgtggtgc cgagcgggcc aacgtgcgtc aagcgcgccg
     3301 ccaaatcggc cgcacgcatg gccgggccgc gactggccgc cgcgaagtac aggcgacgca
     3361 cgtccgccgg cgtaactcgt tccaaccaac cggagactgg atcggcggag ttgaataccg
     3421 cgacggcgcc tgagggcaag tgcacgaaca gctgcgcttt gctggctaag tagtgttcga
     3481 aggaaccgtg cgcattgaga tgatcatggc tcaggttcgt aaataccccg gcgtcgaaac
     3541 gccagcgttt ggcccagccg ctcgccagcg cctcactggt cacctcgagc gccaggtgcg
     3601 tcactccggc gtcggcgtgg ccgcgcatcc ggtcgaagaa ggtgctctgg ctgggtgagt
     3661 cgtactcacc cagccgcgct cctaccccga tcgtcgtctc gctcgccacg gccaaacccg
     3721 cggctcgcag cacctcggcg acgaacaacg tggtgctggt cttgccattc gtgcccgtca
     3781 caccaatgct gtgcaagcct gacgcccaag gagcgtaggg tgccgatgcg ggtgcgaggc
     3841 gaccagtcat ttccgttcag ggacctgcgg ggcgtaaaga atcgatagat atcgaatcta
     3901 gtcctggctc tcaatccgtg cagctcagga atgcctggcc acagtctccg ccgcccgttc
     3961 ccgtagatct aacggtgcac tcgcggtaga gctcgagcgt cgtctcccac agcacgccgg
     4021 ttccatacac atcgacccgc aagaggcagg ttcggcgctc gatcccggcg cattgggcca
     4081 tcaaggaatc acaagccgcc tctcgagtga ctgcacgttc ggggatgccg cggcaggctc
     4141 gatacgagta ctcgcagctc acggcctcgt caccgtcgag gcagttctct atatcggcga
     4201 tcttttcgtc ggagagaggg ctcaagtcgc ggatgcactg cgattgggga atctcggcac
     4261 agttgacctg gagctggtca cacagggctt cggctcgcgg cgaggcgcag gtctgggcca
     4321 gcatcttggc ctcgcagtcg tacgcggcct ccgcggcggc cagcgtgcac gccgagccgt
     4381 cacttccgag cttcgcgaga cactcagtca agacttgcac tgagccggtt cgctgattgg
     4441 cgtagtactc gcacagctgc atgcccacga ctggcacctc caaaccctcg cccgtgggat
     4501 tggggcacat ggcctccgca aacggcaggc tcgcacaatc gaaaccgagc ccctcctctg
     4561 cgggggcgcc gcttacgcag ctactcacgc cctgcccgcc ggcacccgcg tctgccatgg
     4621 aatcagaacc gccggagcca gaatcggtgc cgcccttgct gtccgcatca gcgccgccag
     4681 cccccgcgtt cacggtgtct tctccacccg agcccccctg gttctccaac gttccgcccg
     4741 agccgccgcc gccctcgccg gcgccgcccg agttcgtctt ttgaccggag gagccgcctt
     4801 gtgcggagct cgacccgcct gaccgtgact ctcgatcttc cccgtccgta acctctacct
     4861 tacagcccgg cgccgccgag ctacccacag ccaaaaggca ggtaatctga agaaaccgct
     4921 cgaatgacaa tcccatctgc cctcgatagc ccagttgttg cgtgaacgaa agcagttcag
     4981 gctcaaatca agccataatc ccggcttggc gccatcgcgc ttgcgctgcc cgggcgtctg
     5041 tccaggttta ggcgctgcgc gtgtcggaaa tcgctgcttg tgcgatcgcg agtgagctgc
     5101 ggcgggcagc catggaagca ccgtccggtc gaagattggt aagactgttc gcccatggct
     5161 ggggttcgcg ccgcgagtga gcctgcagga gcatgagaat aggctcgtct gaagacaaca
     5221 aatgatggga ggcattcatc ggtcgacttc gatgttccgc gtaactaaga tattggaatg
     5281 cgcacgagag tattaatctg gggcggcctc ggctgtttac tgctttcatg ctcgcaagac
     5341 tatgcgggtg gggaggccaa tgatcgatcc cgcgcggctg gcgctagcat aggcacggcg
     5401 gcttccggcg gaaggggagg gacgaggagc ggagttcggg ttgggccaac tcactcccac
     5461 tctgggggag ccgtcaaccg caactccagc gcacctaaca gcagtgacgt tacgggcagt
     5521 tcggagtctg acggaagagc tgcgggaggg cgatcgggaa gcgacgtctc ggaagagcgg
     5581 ggcaccgtac aaagcgagac tttggagtcc gatgacatcg gatgcggcgg aggcggtggc
     5641 ggaggaagcg gaggagattg cggagtgagt gagcctccgg tcgcggcagc gggtggaggc
     5701 tggggctggg gagatggaat gggc
//
LOCUS       DASTKU010000834         5849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1726443, whole genome shotgun sequence.
ACCESSION   DASTKU010000834 DASTKU010000000
VERSION     DASTKU010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726443"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1211..2020)
                     /gene="map"
                     /locus_tag="VFQ61_29180"
     CDS             complement(1211..2020)
                     /gene="map"
                     /locus_tag="VFQ61_29180"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET9958615.1"
                     /translation="MLFRNRSVHVKTAREIEHMRVVGRLAAETLHAVGSIIRPGITTE
                     EINDFVHKDTLRKGARPAPLNYLGFPKSVCTSINEVVCHGIPGPRKLLEGDIINVDVT
                     HIFQGFHGDTSATFYVGTPSEDAKKVTEIARRCLDLGIAEVKPGARLGDIGAAIQEFA
                     EGLGCGVVTDFVGHGIGRKFHEEPKVSHVGVRGRGERLRPGMTFTIEPMINLGTHEVE
                     VLEDDWTAVTADGKLSAQFEHTVLVTDDGFEILTQRPGQLENSEIFPNYWG"
     gene            2310..3086
                     /locus_tag="VFQ61_29185"
     CDS             2310..3086
                     /locus_tag="VFQ61_29185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958616.1"
                     /translation="MKRVACSLLAIVLWVPSGNLVAKAEEGVFPGLHPASSGARTLEL
                     DADGVAFGSNSEQVARLYDRYWERHFVARYRKTNPGPKSAELDYELAERKKVLRRVTT
                     FDLRSTTYDTSEFKDEFARGNGETMTSVEVLREGTGSKMVSYTRRFFFAQDRLWKTYD
                     EYRLEPQGPWGADFKEAVERVAASLGPSAKRTRAPESPWETVAFEAGTLRVRLIKLPK
                     DRVALVRADAALAREVLDRRAHEVREVPDRLDPDTQAALR"
     gene            complement(3175..3486)
                     /locus_tag="VFQ61_29190"
     CDS             complement(3175..3486)
                     /locus_tag="VFQ61_29190"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET9958617.1"
                     /translation="MRDEILTVIREVLAPMVVADGGRLYVIQADAERVALHLAGRFAG
                     CPGNQVATQRVIQPAIAAVAPQAELTVTWGRLVPSGATRIDAGGPASSPSSSTRNGPG
                     A"
     gene            4098..4355
                     /gene="rpsP"
                     /locus_tag="VFQ61_29195"
     CDS             4098..4355
                     /gene="rpsP"
                     /locus_tag="VFQ61_29195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET9958618.1"
                     /translation="MAVHIRLARHGTKKSPYYRIVVAESRDPRDGRFVERLGVYNPVI
                     SPKTFEIQRARYDYWTSKGAVPSATLVKLLKKYPVKESTAG"
     gene            4891..5187
                     /locus_tag="VFQ61_29200"
     CDS             4891..5187
                     /locus_tag="VFQ61_29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET9958619.1"
                     /translation="MELDRLSQRDLMERFRDDTGMPLKELISTIAQALVDDPDSVEVR
                     EIEGDQNSLIELRVAKGDIGKVIGKEGRTAQSMRTILTAASTKLGRRAHLDIVD"
     gene            5337..>5849
                     /gene="rimM"
                     /locus_tag="VFQ61_29205"
     CDS             5337..>5849
                     /gene="rimM"
                     /locus_tag="VFQ61_29205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET9958620.1"
                     /translation="MNERRELLRIGRVRRAHGLTGELEVRLDWEGSDGLLQASHVYLE
                     LADGRRQEREVLRARATPKGVLLLLQGIADRTSAESCVGNIVCVPRGELPELEPGEYY
                     LSDLVGASVTSRDGFSGRVLEVQMYPSVDALVIESGDGERFEQPLLDEWVERVDVAAR
                     EIVLHSRDGLI"
BASE COUNT         1063 a         1791 c         1911 g         1084 t
ORIGIN      
        1 caccctgact tcttcacccg tcgcatcccg aagccagcaa ccgggtcggg ttcatagctg
       61 aacccggcat ccggttcgag caatccacct ccaggcctct ttcgagatcc cttctcctgg
      121 agcgtgacga aggcgtgtca cacgggtggc ttgccctcgc cgagatgcct gcctcccgcg
      181 atcgggttgg ctaccgccgg gtcctttctc gccgcccctg agcgccgact cctgcagacg
      241 tggctcagag ctttcttacg ccgagtttct tcggcggccc ctcgaacgcc catctggttc
      301 gtgaggatcg gaccgagact acgttgagcc cgacgaagcc ggtttctctc gtgctcgatg
      361 cggatacgga ctgcggtcta tgcggactgc ttgctttgat tcgagagttg actggatcgg
      421 aatgcttgat tcaaagctcc cgcgaccaag ccactagcgg ccgaatcgcg atcgtatagc
      481 cactcgtttc aactgggtaa ttgcgactag gtaattgcaa ctaggtaact gcgactgggt
      541 agttgcgatc cccggcaatt gcgatcccca gcagttgcca acgttggtaa ctgccatcct
      601 tggtaattgc gatccccggg taattgcgac gccgagcatc ttcgcacccg acgggcaccc
      661 agcccaaact ttgggagttc gcaagctagt cagccgcaac tagatccgtc aagcggactt
      721 ttgcccgcgt ggccgcccgc ctctgcgagc ctcgcggacg cctgcgtctc gagcgcactt
      781 tgtccgggat ttctgcccat ccgtgcgact ccgagctggc ggagatgtgc gccggaagtt
      841 ggctctagtg ctcgagccat ccatgggcgg ttcagctgtt cacggctgtt cacggccgtt
      901 ccatcgagga aagggtaggg ccaggagcat aaaaacgagc ggccatcggc gtctccttgg
      961 agtcgggaca gggctcattt tgcaggacgc caagccactc cacgcgccac cttcatgaaa
     1021 cgacatggct cgccgggctt gccggcgcga cgcatcaacg cgcctcgccg cagacgcttg
     1081 cgacactgcg agcggccgtg cgccactcgc tcggtgctca ccaccgcgcg cagctggccg
     1141 ctctaggaag cggccagctc ggcctcaacg gcgagaacac gcggcgcgcc cgcgtcaatg
     1201 gcaataaata tcaaccccag taattcggaa agatttcgct gttttcgagt tgaccggggc
     1261 gctgcgtcaa aatctcgaat ccgtcatccg tgaccagaac cgtatgctcg aactgggcgg
     1321 acaacttgcc gtccgccgtg accgccgtcc agtcgtcctc caggacttcc acctcgtggg
     1381 taccgaggtt gatcatgggc tcaatcgtga aggtcatgcc gggccgcagc cgctcaccac
     1441 gcccgcgaac accgacgtgg ctcactttcg gctcttcgtg aaacttgcgc ccgataccgt
     1501 ggccgacaaa gtccgtcacg acgccgcagc cgaggccctc ggcaaactct tggatggcag
     1561 cgccgatatc gccaagccgt gcacccggct tcacctcggc aatgccgaga tcgaggcagc
     1621 gccgagctat ctccgtcacc ttcttcgcat cttcgctggg cgtgcccacg tagaacgtgg
     1681 cggaggtgtc gccatggaac ccttggaaga tgtgcgtcac atccacgttg atgatgtctc
     1741 cctccagcag cttccgcggc ccagggatgc cgtggcagac gacttcattg atggaggtgc
     1801 agacactctt cggaaaaccg agataattca gaggggctgg gcgcgcccct ttgcgcaacg
     1861 tgtccttgtg cacgaagtca ttgatttctt ccgtcgtgat gccggggcga atgatgctgc
     1921 cgacggcatg gagcgtctct gcggccaaac ggccgacaac gcgcatgtgc tcgatctctc
     1981 gtgcggtctt gacgtgaacg ctgcggttcc gaaatagcat ggtctcgcta gtctgccgtc
     2041 ccgctctgaa ctcgtcgagt gagaatcatc gctgctcgaa ataccggcct gagttcgtgt
     2101 gcaacaggat cttggaatgg tttcggcaca ggcgcgtggc acctccagag gtgagcgcct
     2161 acactgtgcg ggactcgact catgatgcgg gcggtttgcg tcggaagcct ggcggtgtac
     2221 ggctcggtgc cgtgcagtcg tgcaggctgg gagtgcgcct tcagcgcgcg ttttggcccg
     2281 tccgcgccag gaagtgtagg gaatgagcaa tgaagcgcgt agcctgcagc ctcttagcca
     2341 tcgttttatg ggtgccgtcg ggtaatctgg tcgccaaggc ggaggagggg gtattcccgg
     2401 gtctccatcc tgcgagcagt ggggcacgga ctctggagtt ggacgccgat ggagtggcct
     2461 ttggtagcaa ctccgaacag gtcgctcggt tgtatgatcg atactgggag cggcacttcg
     2521 ttgcccgata ccggaagacc aatcctgggc cgaagagcgc ggagctcgac tacgagctgg
     2581 ccgagcgcaa gaaggtgctc cggcgagtca ccactttcga tctgcgctcg accacgtacg
     2641 atacctcgga gttcaaagac gaattcgcgc ggggcaacgg cgagacgatg acctccgtgg
     2701 aggtcttgcg cgaggggacc ggctcgaaga tggtcagcta cacgcgtcgc ttcttctttg
     2761 cccaagaccg gctctggaag acgtacgacg agtaccggct cgagccgcaa ggtccctggg
     2821 gcgccgactt caaggaggcg gtcgaacggg ttgcggccag tctggggccg tctgcgaaac
     2881 ggactcgcgc gccggaatct ccctgggaaa cggtggcttt cgaggccgga acgctgcgcg
     2941 tgcggctgat caagttgccc aaggaccgcg tggccctcgt gcgcgccgat gcggcgctgg
     3001 cgcgcgaagt gctggaccgt cgagctcacg aagtgcgcga agttcccgat cgcctggatc
     3061 cggacaccca ggccgccttg cgctgatccg accggggatc cgctggtcat agagccggtc
     3121 gctgcgatgc acgctcatcg cggcggccga gcttttgcgg ccagacgcgg atcttcaggc
     3181 gcccgggccg ttgcgggtcg acgacgacgg cgaggaagct gggccaccgg catcgatccg
     3241 cgtggctccg gaggggacca agcgccccca cgtgacggtc aactcggctt gcggcgcgac
     3301 ggcggcgatg gcgggttgaa tcacccgttg ggtggccacc tgatttccgg gacaacccgc
     3361 gaatcggccc gcgagatgca gggcgacgcg ctcggcatct gcctgaatca cgtacaggcg
     3421 gcccccgtcg gccacgacca ttggcgccag aacttcgcga ataactgtga gaatttcgtc
     3481 tcgcatggag ctgccttgat cggctcgggt caaatctacc acgggccttg accggacgat
     3541 gagaattccc ttagggtgcc gccgcgaacc cagccaaagc tggccggcgt gcccaggggt
     3601 ggttggtgtc gctgtaccgt cgtggcaggt ggtcgtggcg gttggcttct ggtcgtgata
     3661 actgccctcc ggctgtgaca acggccgaaa ccgttgttgc ccaagtaccg tgatagagca
     3721 cggtgacaaa gcacagtgac tgacaaagca cagtgacgaa gtaccgtgat aaggcaccgt
     3781 gaccaagtcc tgcgacaaag ggcaagaaga acaccatcag aagagcttgc gaagatcaca
     3841 cgctgagagc cagcgcacgc gaggagcgag cccacttccg gcgcacgggg atgcgggcgt
     3901 ctcatcgctc ccggtgagtg tcgagtgagc gtttcccgaa gccgggctcg ccgcaccgac
     3961 ggaacccgtt aaaaatcaat ctgggcaccg cgccctcaga gcgccgcgca tgggaagcgc
     4021 ggccagcaga aaagcattga aaagccttgc cgcggagccc cgtcagaaac accgattccc
     4081 cccaaaactg agtaccgatg gccgtacata tccgccttgc ccgacacggc accaagaagt
     4141 ccccctatta ccgcatcgtc gtggccgaat ctcgcgatcc tcgcgatggc cgcttcgtgg
     4201 agcggctcgg cgtctataat cccgtgattt cgcccaagac gttcgagatc cagcgcgcac
     4261 gctacgacta ctggacctcc aaaggggccg ttccgtcggc gacgctggtc aagttgttga
     4321 agaagtaccc ggtcaaggaa tcgacggcgg gctgaggcct ctcggttcgt tagatttccc
     4381 cgtatcggtc cgcctgggag cagtcgttga gagctccggc gggcctccgt ggtcttcggc
     4441 tcaggtccaa acgggatggg cgctggacct ggcgtgtcgc gaatggcttt cgggaatgat
     4501 ggctccgtcg ctacgaacgt ggatggcgga gccgtcgaac ggacctccgc agtggcggaa
     4561 gatgctccgg ctcgcctgcg ctcctgagga caggagtggg ggctgcgttc cgggcccagg
     4621 aaggctttgg cggacgctct ccgggggacg cagcacgtcg gcgccgaaag cgccgcatga
     4681 aatagaatag ctagtgtggc gtctagagtg ccgcgcggct tggcccgacc cgcgccgccg
     4741 cagccgaacc ggccccgtcg ggccacgatt gctacttatg agacgcgaca ctaggttgcg
     4801 cgcagaggcc gtagacctcc cgagccgtgc caggttcact ggcgcggggt atgctcgccg
     4861 tctactcggt attcggtacg ccggcgagcg gtggagctcg atcggctcag ccagcgggat
     4921 ttgatggaac gttttcgaga cgacacgggc atgccactca aggaactcat cagcacgatc
     4981 gcgcaggctt tggtcgacga tcctgacagc gtggaagtgc gagagatcga gggtgatcag
     5041 aactctctga tcgagcttcg ggtggcaaag ggtgacattg ggaaggtcat cggtaaggaa
     5101 ggtcgcaccg cgcagtcgat gcgcaccatt ttgaccgcgg cgtccacgaa gctggggcgc
     5161 cgcgcgcacc tggacatcgt ggattgaggc gtagcttcac cgagacgcag ttccccgagg
     5221 gcacttttga atagctaaag cgccgggcaa aggagctgag tgcggtggag agcaggctgg
     5281 aatcgatgcc gccgaccgat gcttcgagcg ggcgagccag gtcgcccgag cccgctgtga
     5341 atgagcgccg ggagctgttg cgaatcggcc gtgtgcgccg cgcccacggc ttgaccggag
     5401 agctcgaagt gcggctggac tgggagggca gtgatggctt gctgcaggcc agccacgtct
     5461 acctggagct cgcggacggc cgccggcagg agcgcgaggt gttgcgagcg cgcgccactc
     5521 ccaagggcgt gttgctcctg ttgcagggca tcgcagatcg cacgtcggcg gagtcctgcg
     5581 tgggcaacat cgtgtgtgta cctcgcggcg agctgccgga gctcgagccc ggtgaatact
     5641 atctgtccga tctggtgggc gcatcggtga ccagtcgcga tggcttctcg gggcgagtcc
     5701 tcgaagtcca gatgtatccg tcggtggatg cgctggtcat cgagagcggc gacggcgagc
     5761 gcttcgagca acctctgctc gacgagtggg tcgagcgggt ggacgtcgcg gctcgcgaga
     5821 tcgtgctgca ttcgcgcgat gggctgatc
//
LOCUS       DASTKU010000835         3784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_609874, whole genome shotgun sequence.
ACCESSION   DASTKU010000835 DASTKU010000000
VERSION     DASTKU010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609874"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFQ61_29210"
     CDS             complement(<1..238)
                     /locus_tag="VFQ61_29210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958621.1"
                     /translation="MQQPASRSVKSGVNQNRKTPDGFRQPRLVASRLLCLSGLVVPAA
                     FTGCFSSETEDCVRQCQSSECPGDMQCRDHFCVHP"
     gene            complement(332..1168)
                     /locus_tag="VFQ61_29215"
     CDS             complement(332..1168)
                     /locus_tag="VFQ61_29215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958622.1"
                     /translation="MSQRREELSGQTAARTLRVDPGPQIASLKPQAAQLRPGQRVRIG
                     VLLGLALLTSTRYSQAQSSDWSEGSPKADQSKGLFGPFRIGPLVGFGLPNLINFGVTL
                     KLTRYFGAGVNFGLIPQVRISYYGEAQVSYQEYDAYARVFPFGGGFFLGSGVGYAHAR
                     GSFRKDTDISDYAAQYPALNLPRTLSSTSQGSVQSMVLTPQLGYFQIFRGGFCAGADV
                     GLQIPIAKSRTELDTRVQQSVPEQAIERVVGPGEAEVRDTLKSVARTPIPTFNFRIGW
                     ML"
     gene            1291..1713
                     /gene="trxC"
                     /locus_tag="VFQ61_29220"
     CDS             1291..1713
                     /gene="trxC"
                     /locus_tag="VFQ61_29220"
                     /inference="COORDINATES: protein motif:HMM:NF008229.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HET9958623.1"
                     /translation="MIVECSGCSRPNRLPAARMQERAKCALCKAPLLPPSHPVALGSA
                     ADFEELIRDAPGPVLVDFWAAWCGPCRAVAPELEKLAQSRAGRLVVAKVDTEALQDVS
                     ARYGIRSIPTLVLFRGGQEAERVSGAMPASAIAAKFGI"
     gene            1865..2941
                     /locus_tag="VFQ61_29225"
     CDS             1865..2941
                     /locus_tag="VFQ61_29225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958624.1"
                     /translation="MRWRCSACQTENLGRHKVCQNCTKPKDREPFYDAPEAAQPDPSQ
                     GVSDAGLIAKATAGADWCCRYCGSHQPRNTGECKNCGAPEAEASQPASYVSSTAAASS
                     SSAAQDPNLEPSRPRVRRRYIVLAGLVGLIALLFLVFRSREVEARVVQRAWQHTVVVE
                     RYRIVPGEGFSENRPAEAFDVVQRGERHHHNQRVQDGTVRESYSERVACGEDCSTSPV
                     RCSSNDNGFKTCSGGDRVCRTRYCSETRYRDVPRYKDVPVFQTWYSWRVWAWHTDRTL
                     VEKGTEEAPRWPSEVQLHLGAGCGPGEKERTRQEFRYDVEFETPDHDRYPYVAGNLAE
                     FTQLSLGTQKRLRVGRAHKTEIVP"
     gene            3068..3496
                     /locus_tag="VFQ61_29230"
     CDS             3068..3496
                     /locus_tag="VFQ61_29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9958625.1"
                     /translation="METHELYRGRLIDHIQLVVPDIERSKRFYRVVLESLGFQIGGEG
                     PGYFWADELFVSDMQSPASSNRATGRAHLAFQAKDREAVERFHRAGLSAGGTDNGAPG
                     VRLYHPGYYAAFLLDPDGNNIEAVYHGPAERSAEAIKLTF"
BASE COUNT          746 a         1112 c         1218 g          708 t
ORIGIN      
        1 cggggtgcac gcaaaaatga tcccgacact gcatgtcccc agggcactct gagctctggc
       61 actggcgaac gcagtcctct gtctcgctgc tgaaacaccc tgtaaatgca gccggcacca
      121 cgagcccgga caggcaaagc aggcgtgagg ccacgaggcg tggctggcga aaaccgtcag
      181 gtgttttgcg gttttgattc acgcccgatt tgacggagcg ggacgccggt tgttgcatcg
      241 aacttcccca ctttctagag atccaccgcg gccaccaaag tgcactggat ctcgggggag
      301 aatacgcttc gtgccggtcg atcggaagcg cctacagcat ccacccgatc cgaaaattga
      361 acgtgggaat gggtgttcgg gcgacgctct tcagtgtatc tcgaacttcc gcttcaccag
      421 gcccaaccac tcgctctatg gcttgctcgg ggactgattg ttgaacgcgg gtgtctagct
      481 cagttcggct cttggcaatc gggatctgaa gaccgacgtc cgcgcccgca caaaaaccgc
      541 cgcggaagat ctgaaaatat ccgagctgtg gagtcaggac catcgattgt acggaacctt
      601 gactcgtgga gctcagagtc cggggcagat tcagcgccgg atattgagct gcgtagtccg
      661 agatgtccgt gtctttccgg aagctgcccc gcgcgtgcgc gtagccgacg cccgaaccca
      721 aaaagaaccc gccgccgaag gggaacacgc gcgcgtaggc gtcatactct tgatacgaga
      781 cctgcgcttc accgtagtac gatatccgga cttgcgggat gaggccgaag ttgacgcccg
      841 cgccgaagta gcgggtgagc ttcagcgtaa ctccgaagtt gatgagattg ggtaatccga
      901 acccgacgag agggccaatc cgaaaggggc cgaagagccc cttcgactga tctgccttcg
      961 ggctaccctc ggaccagtcg ctcgactgtg cttggctgta gcgagtactg gtgagcagcg
     1021 ccagacccaa taggactcca atacgaacac gctgcccagg cctcagctga gcggcctgcg
     1081 gtttcaggct cgcaatctgc ggtcctggat ccacacgcaa tgttcgcgct gctgtctgcc
     1141 cggagagttc ttcacggcgc tggctcacgg ccgttaggct atccgatcag cacgcgcctc
     1201 gcatccgcct cgagcggggg gaacgagaga tgctgcggaa atcgacgcat gccttgacgg
     1261 atgcgtagcc agccacacgt tggtccaagc atgatcgtcg aatgctccgg ttgctcgagg
     1321 ccgaatcgct tgcctgctgc tcggatgcag gaacgcgcca agtgtgcgct atgcaaagcg
     1381 ccattgttgc cgccgagtca ccccgtcgcg ctcggcagtg ccgccgattt cgaagagttg
     1441 atccgagacg cgccgggacc ggtattagtg gacttttggg cggcgtggtg tggaccatgt
     1501 cgggcggtgg cgcctgaact cgagaagctc gcgcaaagtc gtgcaggccg gctcgtcgtg
     1561 gccaaagttg acaccgaggc cctgcaggac gtctcggctc gctacggcat tcgaagcatc
     1621 ccgaccctcg tcctgttccg cggtggccag gaggcggagc gcgtgtccgg tgccatgccc
     1681 gcgagcgcca tcgccgccaa attcgggata taggtgggcg ataagcgtgg ggagtaagag
     1741 acgcggttcg acgaccgcct tggagtccgc gtgcaagcgt tccaggtgag ccgggctcga
     1801 agccgggctt catgccactg aaaaccgagt acaatcagcc cttgtgccgg tttttcatgt
     1861 cgagatgcgc tggcggtgct ccgcctgcca aaccgagaac ctcgggcgtc acaaggtttg
     1921 ccagaactgc accaaaccga aagaccgtga gcccttctac gacgctcccg aggccgcgca
     1981 gcccgatcct agccaaggcg tgagcgatgc ggggttgatc gccaaggcga ccgccggggc
     2041 ggactggtgc tgtcgttact gcggtagcca tcaaccgcgc aataccggtg agtgcaagaa
     2101 ctgcggcgcc ccggaggccg aggcgagtca accagccagc tatgtatcga gtacggctgc
     2161 agcgtccagc tcgagcgcgg cacaagaccc gaacctcgag ccgtcgcgtc cgcgagttcg
     2221 tcgccgctat atcgtgctgg cggggctggt tgggctaatc gcgctcttgt tcctggtgtt
     2281 tcgctcgcgt gaggtcgagg ctcgcgtggt gcaacgcgca tggcaacaca cggtcgtcgt
     2341 agagcgctat cgaatcgtgc cgggtgaggg cttttccgaa aatcgcccgg cggaggcctt
     2401 cgacgtggtg cagcgtggtg aacgacatca tcataaccag cgcgtgcaag acggcacggt
     2461 gcgcgagagt tactccgagc gtgtggcgtg cggtgaagac tgctcgacca gccccgttcg
     2521 ttgcagcagc aacgacaacg gtttcaagac ctgttcgggc ggagatcgcg tgtgcagaac
     2581 acgatattgc tcggagacac gatatcgcga tgtgccgcgc tacaaggacg tgcccgtctt
     2641 tcagacctgg tactcatggc gtgtttgggc gtggcacacc gaccgcactc tggtcgagaa
     2701 aggcaccgag gaggctccgc gttggcccag tgaggtgcag cttcacttgg gagcgggctg
     2761 tggacccgga gagaaggagc gcacgcggca ggaattcagg tacgacgtgg aattcgagac
     2821 tccggatcac gaccggtatc cctacgtagc gggcaacctc gccgaattta cccagttgag
     2881 cctcggcacg caaaaaaggc tacgcgtggg ccgcgcccat aaaaccgaaa tcgtgccctg
     2941 agccagctgc cgataacccg tgccggaaaa catgcgtcaa aaggcgcaga gcaatcgggc
     3001 gaattcgtgg gttttccagg cgagccgcga gttgatggga aaagcacgag gcgctagaaa
     3061 tgtggtgatg gaaacacacg agctctatcg aggccgcttg atcgatcaca ttcaactggt
     3121 ggttcctgac atcgagcgga gcaaacggtt ctatcgtgtc gtgctggaat cgcttgggtt
     3181 ccaaatcggc ggtgaaggcc ccggctactt ttgggcggat gagctgttcg tctcggatat
     3241 gcagagcccc gcgtccagta atcgtgccac gggccgcgcc cacttggcct tccaggccaa
     3301 ggatcgggag gctgtggagc gctttcatcg agcgggtctg agcgcgggtg gtacggacaa
     3361 cggcgcgccg ggcgttcgct tgtaccaccc aggttattac gcggcgtttc tactcgatcc
     3421 agacggtaac aacatcgaag ccgtctacca cgggcccgct gagcgctcgg cggaagcaat
     3481 caaactaacg ttctgaaggg cgttgcggcg agagccgggg cggtatgctg gcgtcgtccg
     3541 tcgtcggcgc gaaaatccgg gatgagggct gagcgtctac gcttcgcgcg cgttcggatg
     3601 cggccctcga ttccgaacac atgctttgtg tttcgtggcc gaatccgccg tgtaataggg
     3661 cattctccta tccggtcgat accattcgcg aaccgagacg gtgctcgaga ctgcggacgg
     3721 cgccctcggg gagctccggg aacggagccc ggttacttga ccggctgaat ctgcgataaa
     3781 gttt
//
LOCUS       DASTKU010000836         8723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1280764, whole genome shotgun sequence.
ACCESSION   DASTKU010000836 DASTKU010000000
VERSION     DASTKU010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280764"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1478..2239)
                     /locus_tag="VFQ61_29235"
     CDS             complement(1478..2239)
                     /locus_tag="VFQ61_29235"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HET9958626.1"
                     /translation="MHSAFQRTNHRPWPVPERAWTWRQSWHDLLFAHWPIPARELRAF
                     VPEPLRVQEFEGTSYVGIVPFRMSGVMRRPFPDLPWVSAFPELNVRLYVEHGGKAGVW
                     FLSLDATNPLAVWAARRFFYLPYYRSTMSLREMPDGDIEYRAARAGAAFRGNYRPTSA
                     PYLAQPGTLEHWLTERYCLYALAPGGRLLRNDVHHVPWPLQRASAEITTNTFPEYHRL
                     PVEGPPPLLHFARRIDVVVWSAETVSGNSSAGSSL"
     gene            complement(2268..2933)
                     /locus_tag="VFQ61_29240"
     CDS             complement(2268..2933)
                     /locus_tag="VFQ61_29240"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9958627.1"
                     /translation="MSRLPPGQSARSDFPRFGLPRYAARSPRSPLSRTLSVSRGEAPA
                     IQLIEPLGGLARIQLQSDFHCVTTWSSLNLSWSGVRFVDLYEAKIAPQLDANATATHA
                     VIRGQDGYRTTLPLKDLLASDVILADQLGDEPLSIAHGAPWRLVAPRHYGYKSVKHLS
                     RIELWSTEPDVRPAAFRFMDHPRARVSQEERGRWVPGWLLRYLYRPLVAGTIRRFASE
                     PRA"
     gene            2993..3823
                     /locus_tag="VFQ61_29245"
     CDS             2993..3823
                     /locus_tag="VFQ61_29245"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9958628.1"
                     /translation="MSSHQVTQNRNQARLGHRLCGSGPRRAIVLNDWLCDTSTWDAAA
                     PYLDVGGFTYAFADLRGYGQSRALIGEYSLREAAQDVGELADSMEWETFHLVGHSMSS
                     LVVLHLAQQFPERVQRVVALTPPPPGGFGASDEMIAASEQLAWGDDAARIAALAQRFG
                     DHWSIGWARYKAARWRATAEAPAIAAYVRLFARDGLVDLQTKIQVPVLAITGERDLPP
                     MRAAAVDRYWRPLCANLAVHGLTDSGHYPMQELPPLTVTLVERFLAGSLGLDFLGVPA
                     "
     gene            3833..4519
                     /locus_tag="VFQ61_29250"
     CDS             3833..4519
                     /locus_tag="VFQ61_29250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET9958629.1"
                     /translation="MSTQQLDPAGDSARGNEPASGNELSIVGRSSSHYTRLARIFAAE
                     LGISYSFQIVHDILSTNPEDYAGNPALKVPALRTNSGAWFGALNICRELARRSKSGLR
                     VLWPEDLRYPLLANAQELTFQAMTTEVALIMGKVAAESGAQAPANLGKMRSSLANSMA
                     WLDEEAPNLWEALPERDLSVLEVSLFCLVEHLEFREILPTSPYSNLIEFCRVFKARPS
                     AQQTSYRFDR"
     gene            complement(4535..5200)
                     /locus_tag="VFQ61_29255"
     CDS             complement(4535..5200)
                     /locus_tag="VFQ61_29255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958630.1"
                     /translation="MDSLTGHFKNMQAQDAPEDTRQKLHELMALVQRSLDTSSTPHLT
                     AISLSSMSEPAAPDSTVDGGVITAHVKPSFKYDRLALADAHRELLLSGGGQLFAAIIR
                     AQRESPAAQWTLTARLVTRAEHEALAQSRKAIDVEVEALLREHFGDSDQAELAFGRGA
                     PGESVRVIREGRTGQEQLPPTPELMQLLQRTEQLYREAGLNLIVADWRLWPSGFEFCE
                     YFE"
     gene            complement(5420..7234)
                     /locus_tag="VFQ61_29260"
     CDS             complement(5420..7234)
                     /locus_tag="VFQ61_29260"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HET9958631.1"
                     /translation="MAQAKNKPKRVRNFDRRLWSRFWRTAKSYWFGDEKWAARGLLAV
                     LVILLLGRTQFAVLFNEQTGEFTSALAAKDSGRFWGSMRVFGLALVGAVPIYALHYWV
                     RDRLGLRWRRWMTHRFLGKYFSNRAFYELTSNDAIDNPDQRIADDINSFTYKSLRFLL
                     EIVSAGLQLVAFSGVLWSISKELVAFLVFYAAFGSAMTFGLFGKPLIGLNFQQLRREA
                     DFRFGLIRVRENAEAIAFYRGEAREAEHVKGRFHEVLRNGQKLVDKTLFLNLFQYAYS
                     FLALAVPSIIVAPRIISGELEVGRAIQAGGAFAAMLSALTVFVDNFESLSGFAAGVDR
                     LHGFQKTLDDHGRPPAAGAPGRIERVQHPALELEAVTLQIPNSERTLARQLSLAVKEG
                     EGLLIVGASGGGKSSLLRAIAGLWNSGSGLIRRPPAEQMLFLPQRPYMILGTLREQLL
                     YPFTDRALSDAELSAVLDRVNLRDVVERCGGFDRELDFAKVLSLGEQQRLAIARVLLA
                     QPRYVILDEATSALDSENEEHLYRALRAMNTTLVSVTHHPGLAKYHGQVLRLTGGGRW
                     SLEEHSSVADRSERERPADRSGVHPRIERAPKSARARG"
     gene            7463..7687
                     /locus_tag="VFQ61_29265"
     CDS             7463..7687
                     /locus_tag="VFQ61_29265"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HET9958632.1"
                     /translation="MQRIPVVSSSVAKVGYDPEQHVLEVEFHGGRVYRYVHVPIAAYR
                     LLLQAPSIGEYINRVIKPRFEAQRVIPESL"
     gene            7965..8285
                     /locus_tag="VFQ61_29270"
     CDS             7965..8285
                     /locus_tag="VFQ61_29270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958633.1"
                     /translation="MRTRVVAYGTAWVWVLCRETRDRKEWRRGGDAKFGAGKVVVSSG
                     GTGRSSSEDEVSANGVEMGYSGARVGGLQLARMCECLREEKGSPAKSDREWRRIPTGE
                     AGCE"
     gene            8266..>8723
                     /locus_tag="VFQ61_29275"
     CDS             8266..>8723
                     /locus_tag="VFQ61_29275"
                     /inference="COORDINATES: protein motif:HMM:NF022812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HET9958634.1"
                     /translation="MKPGASEHGASDQSAPRPELVHAPPGVPLDIASTYQRLLERHRD
                     LSLSDLPRELGDVQRGEPDLGFDPTQAKYYGAVRRELQLTNEEQRTFKRLGFVVADHG
                     QPTSMGAAYYNIFTRDLPVLVTTDSILHAVHVSYDSILESVEQRLLQRII"
BASE COUNT         1634 a         2679 c         2748 g         1662 t
ORIGIN      
        1 cgccttgcaa gctcctactg aagtaggctc tctaaaggct gggccgactg gtatccctga
       61 tccctcgcat ctccaattcc tcgtacgttg ggactctgta tcgcgccgca attagagcct
      121 caaccattgc agtatgaata atcgagcctt gctaacatac ctacattgga gcgcgtgcgg
      181 tcttccctca tccgcagctt tctcaaaggc tctatgaact aagcaatccc tctaatccaa
      241 agacctgcgg cagacaccat ctccgcgcag acagccgtag agccccttcg accgctggcg
      301 ggttctggaa tgatgatgag tccctcggcg agtgtcaacc actaaggtga gcggctcaga
      361 aaacttctgg tgcggcgcaa actatccttt gtttcgccaa tttcatctct gggacgcgca
      421 tagctctgct cgcgtcggat tcgttcgaag gacttgcttc tgtggagcgg gttttgggat
      481 cgcggtctgg aaagccttcg ctctggtgga cgggcctatg ctgccgtgga ctagctacac
      541 ggcggcgccg agatgcgcag tgagcttgcc gggcgcgtcg ctggctcatc ttcgagatcg
      601 aatgtgagcg ctcggagctg agccaaaatg atgtccagtg tgggtgcgga acgtggatgg
      661 atggcctata tccagcctga ttccgcttga ttcatagccc gctccgtagc ccgctccgta
      721 gccagccgtg gaggcccgaa gggtctgtcg agatgaatgg ctgagcaaaa acggctctgc
      781 cgacagcgtg gagtcacctg cgcgctcgcc gaccgtcctg ttgttctgag ccggcagtgt
      841 ttgagccagc agtgtttgaa ccaggttgtc ctacgctgcg ggttgctctt gagtgcatct
      901 ttgctctggc tttcaggctc gttgagccga ccgccagcat tcctggggtt gatgtcgcca
      961 ggtgtctagt atttggccga ttggtttgct gggaccaaca ctacaaggtt gcttgtgatg
     1021 ggttgttgaa ctcgcggttc agctcagttc gttcgactgg ttggcccgga ttgggcgcgc
     1081 aggggctagc caattggttt atcaaccggt agttaccttc ctcgaggact cgtcggagct
     1141 gatcctttcg actgcggact tcgcgtctaa aaccgggcgg ctactcaggc ccagccgaga
     1201 ttggcatcca actgggtttc gcatcggacc gctttagcgg cgtcggcaac ggcggtgcgg
     1261 gcttgtgacc tcgggcgtcg tgagattcac cagcgtccgg cggcccgcgc gtcgcttcgc
     1321 ggataagcgt acatagaatg gtgaaatgga cgcgaacgga agaggcgtgt tgcgattgga
     1381 ttccacgcgg cggagattcg atatctgaaa acgtggtgtt gctgccgggg gcggcgcggt
     1441 ttcgcgacga gcgagagctc gcggcccgag ttcggggtta caaggatgaa ccagcggagc
     1501 tgttcccgct cacggtctcg gcagaccaga cgacgacgtc gatgcgccgc gcgaagtgta
     1561 ggagtggggg cggcccttcc actggcagcc gatgatactc ggggaacgtg ttggtcgtga
     1621 tctctgcgct cgccctctgt agcggccagg gcacgtggtg gacgtcgttg cgcaagaggc
     1681 gcccgcccgg tgcgagagca tacaagcaat aacgctcggt gagccaatgc tcgagtgttc
     1741 cgggttgggc gaggtaggga gcggaggtgg gacggtaatt gccgcgaaat gcggcacccg
     1801 cgcgggccgc gcgatattcg atgtcgccgt cgggcatctc gcgcaggctc atcgtggatc
     1861 tgtaatacgg caagtagaag aagcgccgcg ccgcccagac cgcgagcggg ttcgtcgcgt
     1921 ccaagctcaa gaaccagacg ccggccttgc ctccgtgctc gacgtacaaa cggacattca
     1981 gctccggaaa tgcagagacc cagggcagat cggggaaggg gcggcgcatc actcccgaca
     2041 ttcggaacgg cacgattccg acgtacgaag tcccttcgaa ctcttgaacg cgcagcggct
     2101 cgggtacgaa cgcgcgcagc tcccgcgccg gaatcggcca atgtgcaaat agcaagtcat
     2161 gccaagattg ccgccaggtc caggcgcgct caggcaccgg ccagggtcga tgattcgttc
     2221 gttggaaggc tgaatgcatg aaaggcctag aggtctagcg ctgtacgtca ggcgcgtggt
     2281 tcactcgcga agcgccggat ggttcccgcg acgaggggtc ggtacaagta gcgcagcagc
     2341 cagccgggga cccagcgacc acgttcttcc tggctgacgc gggctcgggg gtgatccatg
     2401 aagcggaacg cggccgggcg cacgtcgggt tcggtcgacc agagctcgat gcgcgacaag
     2461 tgcttgacgg acttgtagcc gtagtggcgt ggcgcaacca agcgccaagg agcgccgtga
     2521 gcgatgctca agggttcatc cccgagctgg tcggcaagga tcacgtcact cgcgagcagg
     2581 tctttcagtg gcagcgtggt tcgataacca tcctgtccac gaatcaccgc atgcgtggcg
     2641 gtcgcgttgg catcgagttg cggcgcgatc ttggcttcgt acaggtcgac gaagcgcacc
     2701 ccgctccagc ttagattcag actggaccag gtcgtgacgc aatgaaagtc gctctgcagc
     2761 tggatgcgag ccaggccgcc cagaggctcg atcagctgga tcgcgggcgc ttcgccgcgc
     2821 gatacggaca gtgtccgtga caacggcgat cgcggtgatc gcgccgcgta acggggcaac
     2881 ccgaagcggg ggaaatcgga gcgcgccgac tgacctggag gtaacctgga catccgcgca
     2941 ttcgagtgcg gacggccgtc cctcgtcaag ccgcgcatga tagaagcgac gcatgagctc
     3001 ccatcaggtg acccagaatc ggaaccaagc gcgcttggga caccgccttt gcggctcagg
     3061 gcctcggcgc gcgatcgtgt tgaatgactg gctgtgtgac acttcgacgt gggacgcggc
     3121 ggcaccctac ctcgacgtgg gcggcttcac gtatgctttt gccgatctgc gcggatacgg
     3181 tcaatcacgc gctctgatcg gggagtacag tctgcgcgaa gccgcgcaag acgtcggtga
     3241 actagcggac tccatggagt gggagacgtt ccatctcgtg gggcattcca tgagctcgct
     3301 cgtcgtgctt cacttggcgc agcaattccc cgagcgcgtc cagcgcgtgg tcgcgttgac
     3361 tcctcccccg cccggaggat ttggagccag cgacgagatg atcgccgcgt ccgagcagct
     3421 ggcatggggt gatgacgccg cgcgaatcgc ggccctcgcc caacgcttcg gcgatcattg
     3481 gtcgatcggc tgggcgcgct acaaagccgc gcgttggcgc gcgaccgccg aagctccggc
     3541 catcgccgct tacgtcaggt tgttcgcgcg ggacgggctc gtcgacttgc agacgaaaat
     3601 ccaagttccc gtactcgcga tcacggggga gcgagatctg ccaccaatgc gcgcggctgc
     3661 cgtcgatcga tactggcgtc cgttgtgcgc caacttggcg gttcacggcc ttacagactc
     3721 cggacactat ccaatgcaag aacttccccc gctcaccgtc acgctcgtgg agcgtttttt
     3781 ggccggctct ttaggcctgg attttctcgg agtacctgct taagagaatc gaatgtcgac
     3841 tcaacaactc gaccccgcgg gcgactctgc acgcggaaac gagcctgcgt ccggaaacga
     3901 gctcagcatt gtcgggcgat cgagctcaca ctacacccgc ctggcgcgga tcttcgcggc
     3961 cgagctgggg atctcgtact cattccagat cgtgcacgac atcctgtcca cgaaccccga
     4021 ggactacgcg ggtaatccgg cgctcaaggt acccgctctg cgcaccaact cgggcgcgtg
     4081 gttcggtgcg ttgaacatct gccgcgaatt ggcaaggcgc tccaagtcgg gtttgcgcgt
     4141 gctctggccc gaagatttgc ggtatccgct gctcgcgaat gcccaagagc tgacgttcca
     4201 agccatgacc accgaggtcg cgttgatcat gggtaaggtg gccgccgaga gcggcgcgca
     4261 agcgcccgcg aacctaggca aaatgcgcag cagcttggcc aacagcatgg cctggctcga
     4321 cgaagaagcg cccaatctct gggaggctct gccggagcgc gacttgagcg tgctcgaagt
     4381 ctccttgttc tgtttggtcg aacacctcga gttcagggag attctgccta cctccccata
     4441 ctcgaacttg atcgagttct gccgggtgtt caaggccagg ccgtcggctc aacagacgtc
     4501 ctatcgcttc gaccgctgag cgctgacctg cggctcactc gaagtattcg cagaattcga
     4561 agccgctcgg ccagagcctc cagtccgcga cgatcaggtt caagcctgct tcacggtaga
     4621 gctgctcggt gcgttgcagc aactgcatca gctctggagt cggcgggagc tgttcctggc
     4681 cggtgcggcc ttcacggatc acacgcacgc tttcgccggg cgcgcccctg ccgaaggcga
     4741 gctcggcctg atccgagtcg ccgaagtgct ctctcaatag ggcctccacc tcgacgtcga
     4801 tggccttgcg agactgggcc aaggcttcgt gctccgcccg agtgacgagc cgggccgtca
     4861 gcgtccactg cgcggccggg ctctcgcgct gcgctcgaat gatcgccgca aacagctgcc
     4921 cccctccgga gagcaatagc tcacggtgag catcggcgag cgcaaggcgg tcgtacttga
     4981 acgacggctt tacatgggcg gtgatcacgc cgccatccac cgtcgagtcg ggcgcagcgg
     5041 gttcggacat cgagctgaga ctgatggccg tgaggtgcgg cgtgctactc gtgtcgagtg
     5101 accgctgaac cagcgccatt agctcatgca atttctgccg ggtatcctcg ggagcgtctt
     5161 gagcttgcat gtttttgaag tgccctgtga gcgagtccat ctagtgaatc gtcacaatca
     5221 atccgacttg tcaatcgcgt cgaaacgccc gtccgccacg gccggatcta tccatccggt
     5281 gcgcgggatc ttggcgtgaa cgtggggctc ttggcgccgt cgcgccattg gcgaaatgca
     5341 cccgaaatca ccggagtact agcgaaggct caccgcggca aaagctctcc cgagctggag
     5401 cacggctgct tgcgcaagcc tatccgcgag cacgcgctga cttgggcgca cgctcgatgc
     5461 gcggatgtac gccactccga tcagcggggc gctcgcgctc gctgcggtcc gcgacactac
     5521 tatgttcttc caagctccag cgcccgccgc cggtcagtcg cagcacctgc ccgtgatatt
     5581 tcgccaagcc cggatggtgg gtcacgctca ccagcgtcgt gttcatggcg cgcagcgcac
     5641 ggtagaggtg ctcctcgttc tcactgtcga gcgcactcgt ggcctcatcc aaaatcacgt
     5701 agcggggctg cgccagcagc acccgggcaa tcgcgagccg ctgctgctcg ccgagtgaaa
     5761 gtacctttgc aaaatcgagt tctcgatcga acccgccaca gcgctccacc acgtcacgca
     5821 aattgacgcg gtccaagacc gcgctgagct ctgcgtccga gagcgcccgg tcggtgaagg
     5881 gatacagcag ctgctcacgc agcgtcccga ggatcatgta cggccgctgc ggcagaaaca
     5941 gcatttgttc agcgggaggg cggcgaatga gtccggaacc cgagttccag agcccggcga
     6001 tcgcgcgcaa gagagagctc ttcccgccgc cgctcgcgcc gacgatcagc aaaccttcgc
     6061 cctctttcac cgccagcgag agctgccttg cgagtgttcg ctccgaattc gggatctgca
     6121 gcgttacagc ctcgagctcc agcgcgggat gctgcacacg ttcgatgcga cccggggctc
     6181 cggccgccgg gggccgaccg tggtcgtcca aggtcttttg gaagccgtgt aagcgatcca
     6241 cgccggccgc gaaaccgctg aggctctcga agttatctac gaacacggtg agggcgctca
     6301 gcatcgccgc gaaagctcca ccggcttgga tggcgcgtcc cacctcgagt tcaccggaga
     6361 tgatccgcgg cgccacgatg atgctgggca cggccagcgc caagaagctg tatgcatact
     6421 ggaacagatt caggaacaac gtcttgtcca cgagcttctg cccgttgcgg agcacctcgt
     6481 ggaaacggcc cttcacgtgt tcggcctcgc gggcctctcc tcggtagaag gcgattgcct
     6541 cggcgttctc tcgaacgcga atcagcccaa atcgaaagtc tgcctcgcgg cgcagttgct
     6601 gaaaattcag cccaatcagc ggcttaccga acaagccgaa ggtcatggcc gagccaaacg
     6661 ccgcgtagaa caccaggaac gctacgagct ctttggagat cgaccacaat acgccgctga
     6721 acgccaccag ctgcagaccc gcgctcacga tctcgagcag aaagcgcagt gacttgtagg
     6781 tgaaagagtt gatgtcatcc gcgatgcgtt gatccggatt atcgatcgca tcgttcgagg
     6841 tcagctcgta gaacgctcga ttcgagaagt acttgccgag aaaacggtgc gtcatccagc
     6901 ggcgccaccg caggccgaga cgatctcgga cccagtagtg gagcgcgtag atgggtaccg
     6961 cgccgaccag cgcgagcccg aacacgcgca tggaacccca gaatcggcca ctgtccttgg
     7021 cggccagagc ggacgtgaat tcgccggtct gctcattgaa cagcaccgcg aattgagtgc
     7081 gccccaggag cagaatgacg agcacggcga gcaagccgcg ggccgcccac ttctcgtccc
     7141 cgaaccaata cgactttgcg gtgcgccaaa aacggctcca aagccttcga tcgaaatttc
     7201 tgacgcgctt gggcttgttt ttggcttggg ccacggtgga gccctactcc actgcgaatc
     7261 ccgtgccgcc gtcgtgggcc gtgttttgct gcagattcag accggggcat gcgcgagagc
     7321 cctgggcctg ttgcgcagcc gtgtgtcgtg gctgtgccaa tcaccgcgca gaaaacaatt
     7381 acgcctcatc agcgcttctg cgcgaccgcg ggcaacgcat tgcttgcaac actcgtcgca
     7441 agttcctaaa agcgcggagc tgatgcagcg cattcccgtc gtgtcttcca gcgtcgcgaa
     7501 ggttggctac gaccccgagc aacacgtact ggaggtagag ttccacggcg ggcgagtcta
     7561 tcgctacgtt cacgtgccca tcgcggctta tcgactgttg ttacaggccc cctcgatcgg
     7621 tgaatacatc aaccgagtga tcaagccccg cttcgaggcc cagcgcgtga ttccagaatc
     7681 gctgtgaagc cgcagcctcc agatggacgg tcgttgcgcg cagatgtccg acagctgtcc
     7741 tggtccaggg gcctgtatgt tgggcttcga ttgtgacgag cgcgaagctg ctcaggttac
     7801 ctcctcggcg cagtcatccg agcccaccgc gccccaatcg ccgcgccggc ccccccggtt
     7861 taccccgaag atgcggccgc aaaagatttt ctgaaacttc cgcggcctct caccgtccaa
     7921 ccccgtcgtc cagcgagagc ttcggtggac tcccgggtga acgagtgcgc acccgggtgg
     7981 tggcttacgg gacggcttgg gtttgggttc tttgtcgaga gactcgtgat cggaaagaat
     8041 ggaggcgcgg tggagatgcc aagttcgggg cgggcaaggt tgttgtcagc agcggcggaa
     8101 ccggcaggag ctcgagtgag gatgaggtct cggccaacgg ggtcgagatg ggctactccg
     8161 gggcgcgcgt tggcggtttg cagcttgctc gcatgtgtga gtgcctgcgg gaagaaaagg
     8221 gaagccccgc caaatcggat cgcgaatggc gccgcatccc aaccggtgaa gccgggtgcg
     8281 agtgagcatg gcgccagcga ccagagcgcg cctcgccccg agctggttca cgctccgccc
     8341 ggcgtgccgc tcgacatcgc gagtacgtat caacggctac tcgagcgaca tcgcgatctc
     8401 tcgctttccg atctgccgcg agagttagga gacgttcaga gaggtgagcc cgacctcggc
     8461 ttcgatccga cccaggcgaa gtattacggg gcggtgcgcc gggaactgca gctcaccaac
     8521 gaagaacaga ggaccttcaa gcgcttgggg ttcgtggtgg cagatcacgg ccagcccacg
     8581 agcatgggtg ccgcttacta caatattttc acacgagacc tgcccgttct cgtgaccacc
     8641 gactcgattt tgcacgccgt gcacgtgtct tatgactcga tcctcgaaag tgtggagcag
     8701 aggctgcttc agcgcatcat caa
//
LOCUS       DASTKU010000837        28485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1505741, whole genome shotgun sequence.
ACCESSION   DASTKU010000837 DASTKU010000000
VERSION     DASTKU010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 28485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28485
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505741"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /gene="tsaE"
                     /locus_tag="VFQ61_29280"
     CDS             <1..447
                     /gene="tsaE"
                     /locus_tag="VFQ61_29280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET9958635.1"
                     /translation="AALLAPLLAPSDLVVLSGPLGSGKTFLCRALARALGVTGRVTSP
                     TFGLIHELEGSQRIVHADLYRLKSEAELVHLGLDALRDEGRLLLVEWGEDYARALGGD
                     ALYVRLALDPRRATCVASGARSEQLVEQLSARAAAEATVDSGARDG"
     gene            440..1249
                     /locus_tag="VFQ61_29285"
     CDS             440..1249
                     /locus_tag="VFQ61_29285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9958636.1"
                     /translation="MAEAEEVTPSASAGWINLLASPVLRLLEQVGMTVELTGRTLVWL
                     IRPPYRLNLLFSAMDFVGVQSIFIVSLTGIFSGMVLALQTTHSLRQFGAEGVVGSLVA
                     ISLTREISPVFAALMVTARAGSAMAAELGNMRVTEQIDALVTMGVSPIQYLLTPRLAA
                     ATVMLPLLCVLYTCVGMVGAWVVAVQWLGVDPGTFLSNIEKYFVPKDFVMGEIKAAVF
                     GFLISAVACSQGFHASGGAKGVGEATTRAVVQSAVAILIANYILTSWLTDM"
     gene            complement(1300..3273)
                     /locus_tag="VFQ61_29290"
     CDS             complement(1300..3273)
                     /locus_tag="VFQ61_29290"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9958637.1"
                     /translation="MPRPSANGDAASRVASALHAQPTAVAPGTPRLFPSLRTRRLLAG
                     ALLLLPSISVVSLDYLRRGPRIRAFEPEYVLTYSAAVVESMLVWAALVYAASRRRGRL
                     RWLNSVLFVIAFTFTMGGQSYFFQQYNAYLNVDVSVFASNFKDSVLNQLFADIGNYAR
                     AKLPPFIGALLLLWFARRLLRPRRRFALIVGALAPLLLIGSFFIPTQHRHAQASTPDV
                     LYLHAVGGLLRTQFGLTDESNQVRPRARASLPVPPLARAAEAPPRNVVFVILESVRAD
                     SVCIEPDPDCRKTHSTNLLFPDRFPLTQMRSMDSCTAISLAVLWSGVRPTESREVLHT
                     WPLVFDYARAAGYDTAFWTSQNMMFGNARLWVKNLGVSNFASATDLEPTSDLDMGAPE
                     GLLARHVKAQFAQLKEPFLAVIQLSNVHYPYFVDENLPAPFEPWTTSKAPEDNPYFKN
                     YYQNSVYQQDLHVAEFLSHIKQSAAGPRTVVVYTSDHGEAFREHGQMGHTFSIFDEEI
                     HVPAWIDAPEGTLTEPERAALRGKRDAYTFHVDLVPTVLDLMGVVDAPQIAEYRARLP
                     GHSLLRPELTDTALPMTNCAGVWSCAFENWGYMRGSLKLEARSWDQGYRCFDVATDPG
                     EHTDLGLEACQSLLPLARQTFGRLPGQGIERSE"
     gene            3471..4574
                     /locus_tag="VFQ61_29295"
     CDS             3471..4574
                     /locus_tag="VFQ61_29295"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET9958638.1"
                     /translation="MARSSISLLFILLATFGCKTTETAQPEKPSAKAKSTAKPTSVKA
                     ASSKSKAKEPEHAADEKADAHKADAHKSEAHERPELPQKYVVPFAWEASRDEPLSQAR
                     GFLKEILGDNRIYMEHGSAFFAAFAEAQKPRATVVTCSDSRVHTTAFDVTPENDDFMI
                     RNIGNQLANGLGSVEYGVEHLQTPVLMVLGHTGCGAVKAAMGDISGLAEPIRHELEPL
                     HVSRAKAGATPEAAWAEAVVENVRSQVSFALEHFGERVQQGKLTVVGAVYDFRNDLGN
                     GAGKLTVVDVNGNSESERMEAFVQAVETDPMTGKKGARRSGRKADVESPEMSTALKRV
                     ILQAREMQSDGKAAGAATEPKNLPAKLHSANEH"
     gene            5321..5617
                     /locus_tag="VFQ61_29300"
     CDS             5321..5617
                     /locus_tag="VFQ61_29300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958639.1"
                     /translation="MASAKAVRGMVLVGGVTYRIVRLAAGSYSVIRILDDVEVGRFEC
                     APKLRVERTCIDPVEFLVIAREAIQTAKTSWVGRAVVDPVAAERRFELPSRPAN"
     gene            6028..6981
                     /locus_tag="VFQ61_29305"
     CDS             6028..6981
                     /locus_tag="VFQ61_29305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958640.1"
                     /translation="MTETPQGTERVIDGTRRVFYDGYWIKAYDAPADTLLAKKRLIEA
                     LTRRLFNHTEHGINIPGTRLDEARRAFDSETDPRRKRVKGAMLAGALFNRATDIFTKL
                     VEIQALGVEIQSDNALMRRCGEHLQEALGLGKLVLHRSGDEGIDELWGEPFKAFAFPI
                     EEFYKSRYIKMALTMRAIDQIADELCLTFANFPEFAGVTELVREFARASKLKCETLRT
                     DKDIFDVWTSFAVSGERLCAFAPQLPSDPNNDVMRRRLKKGLRLLHSGKELVCYIARA
                     RVPMPKSTHALIERCIRYRNEMDSPRRSRPSERPAARTLSR"
     gene            complement(7028..7984)
                     /gene="nadA"
                     /locus_tag="VFQ61_29310"
     CDS             complement(7028..7984)
                     /gene="nadA"
                     /locus_tag="VFQ61_29310"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET9958641.1"
                     /translation="MANPSTAQSISQSLDLDEEIRRLKRERNAVLLAHYYQESEIQDL
                     ADFIGDSLQLAQAAKGTQADVILFAGVHFMAETAKILNPDKIVLVPDMKAGCSLADGC
                     PVDRFRAWKARYPGSVVISYINCSAEVKAESDYICTSSNAEKIVRAVPADKQILFAPD
                     KNLGRYLIKKTGRDMKLWQGSCIVHETFSLKKLEMLMHEHPDALVIAHPECEEPLLDR
                     AHFIGSTAALLKYVETQPAQTFIVVTEPGILHQMQKRAPHKQLIPAPPDANCACNECP
                     FMRLNTPEKVYEALDKLEPRLEMPVELRERAKIPIDRMLALS"
     gene            complement(8064..9068)
                     /gene="thyX"
                     /locus_tag="VFQ61_29315"
     CDS             complement(8064..9068)
                     /gene="thyX"
                     /locus_tag="VFQ61_29315"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET9958642.1"
                     /translation="MSETQRPVSEGAEQLLGRYFPVLDHGFVALVDYMGTDECIERAA
                     RVSYGYGTRKTSQTRGLLRYLRRHYHTTPSEMVELKFHCSMPMFIARQWIRHRTANVN
                     EYSGRYSLMPMLFYTPEASEFQQQSRTNNQGRSGQAAPSATYAEARKRWEEIRRLSRD
                     TYEWLAGEQVARELSRIDLPLSTYTQWYWKIDLHNLLHFLRLRVDPHAQWEIQEFGRV
                     MAGMLKRIAPMSYEAWIDYDVCGSRLSRMELEALRKLVQLEDEALSGKPGARLDQAAL
                     EQLGLGRREIRELRDKLAAHSVPDFELDLSKGLSPDVFRERCQAAVPTIDQQNAPSGG
                     "
     gene            complement(9168..10760)
                     /locus_tag="VFQ61_29320"
     CDS             complement(9168..10760)
                     /locus_tag="VFQ61_29320"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9958643.1"
                     /translation="MEDTRIYFTRRDCLKVVISGSLGLGSGCKHADSRPEPSRADAAA
                     SAAHLEPGLHAAHAVAEPRVHGEDFTHGHAKRDGHRFPKAERSEKCEVVIVGGGPSGL
                     CAAHLLAKRDVILLEKESRYGGNCSTDTWEGVPFSTGAAFFSEGDTELVELLQSVGAP
                     GQKIEGGDALIVKGQPYFDFFGAGAPRLPLPQRARDDFQRSFAEAVRLREARDSHELD
                     TLRFADFLAPYGPELKQFWQRYAASNWGGAPEHTSMRLGVQAYGWLGGAERRLTYPGG
                     LGVAARALSRAVHQELGPRLRQNSFVHHIETAGDEVHVHAISAGEPVLLRARAVILAI
                     PKFLAARLVPELAAEQREAMQAFRYAPYAVINVCLNQRGPEPAYDNWFLDTPFSDFIP
                     ADWVTRAGRGEKHEKSVLTIYHPLPEAQRRDLLDDQTLMALTEQTLTHLDRHFPGLAR
                     TTAELRVFRRGHALAIPTPGQLSRADIAARHHGRILFAHSDSRGDVSSLPGALRSAKH
                     AVRAYEQLTPRRAVRRDRHQFG"
     gene            11397..11894
                     /locus_tag="VFQ61_29325"
     CDS             11397..11894
                     /locus_tag="VFQ61_29325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958644.1"
                     /translation="MGTSRRKLGTALVALGAGCLVGMAGSWLDLQLSQHLQPVPLAAQ
                     AACDHAPCSPRAAFGQALRIDALGNTSDSASADQLGAVTGGTEPDSQVMSAPGAHLAK
                     RSRLVRSRGWPLLVGEAIDPWEDGAVALASAQFRRRTVRSSSREVSWAYRRDVVDPWA
                     GPTLR"
     gene            12306..15071
                     /locus_tag="VFQ61_29330"
     CDS             12306..15071
                     /locus_tag="VFQ61_29330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958645.1"
                     /translation="MNVLGVERDGTRRLVVLGLRALAHPSGAIEVASEFLPTAKAVQV
                     LSLPERLGGGFLFWAVASRKTQLWRAESFTGTLEPFAELDFEAERLVSGFDRLYVRSR
                     TSSEWFALDAESGAGLGRGNLPTSPSHGEMAFADEWLAAVEVPARGVLVSFDAGGTWH
                     PLGRPFAIEGELEGGVILSLGRQRYRLSASGSLLPEPSTPTPPRELTPSLPNRFAGLG
                     SPLAAALLRGYAAPASAGPRTAREAWVALRGALARVDLGDGRVIETLERPELRDSECT
                     ALTLGTGFGFVCQGARGETRVLAARKDMALELVQSFPTARSVISSGNGGLVWRGECAP
                     SANSTAAAGVYCVRAPSGSSFEVVLRGDRETARVLALSDGRAGVLVTPQPGRKGSLSR
                     VDAHGKARTLPLKLLGDDPKARELFESGFWLETWVERVDGQLSGWVTNRGSFAGVRVL
                     PDGGVKIGPSRRGIERALLSGERALWVPGTGGAEETRDGGMSYHDVDLPLELTAESAK
                     AATLSGLEQGCSLLGCVYAGWAHVGAGGSEDPPHLGVASSPKSTRFPQPGGGRWILRC
                     SLTGDASRVVPAAAQSTSEESGAPTWGPFLESPPPARAQDVVGFNVSGEGGAMRAYAW
                     AAKGADFKKAGRFAVSVLDRYRTTQGVWSTAFAPSPWSDVAQVSELFGYDGATPSAWR
                     VLLDPGGRAGVLSVSVRGNTDLFAVEENLAPTYLGSGSRQGITSLSSAVKLGTIYYVA
                     TYEDPRTLRIFQVSEGRTKLVGRYTDLPEGRGVSMSLVRSAAGDALGLWARSGAGWYV
                     FPIDLATGRAGAPTEVDSASLSKLPRSCAVDEEGYLLDGPVGVEPYVDFVGAADRMSA
                     HGYVGRFILGQRDICLSELSAIADGAVDRRLTPEAKSAPEQQMRVPMTVVDRTEHGRR
                     WGFRCGS"
     gene            complement(15311..16177)
                     /gene="proC"
                     /locus_tag="VFQ61_29335"
     CDS             complement(15311..16177)
                     /gene="proC"
                     /locus_tag="VFQ61_29335"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET9958646.1"
                     /translation="METTKIAMLGAGNMAGALIRGMLASRQVSPEQIRASDVRAERLA
                     ELESEYGIKTHTDNRELIQWANLVVLAVKPQVIDRVLDQLGEHFRPGTLVVSVAAGVP
                     LRALEQRLPSTVRVIRAMPNTAAIVLAGATGIAPGTRATPEDVAITRTLFDATGRSVV
                     LDENLMDAVTGLSGSGPAYVMLIIEALADGGVKVGLHRDTALLLAAQTVFGSAKLLLD
                     TGEHPGRLKDMVTSPGGTAIAGLHTLESGALRKTLIDAVDTATRRAQELGEIMSQRLL
                     GARASTPPPDKK"
     gene            complement(16263..17354)
                     /gene="dnaJ"
                     /locus_tag="VFQ61_29340"
     CDS             complement(16263..17354)
                     /gene="dnaJ"
                     /locus_tag="VFQ61_29340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET9958647.1"
                     /translation="MRDPYEILGIPRSASEAEIKAAFRKLAIQHHPDRNPNDPDAAQR
                     FKELNAAYQILSDPQKRSAYDRYGESAFRPGGAGGVATDFDFSGIEDLFGDLLGAFGF
                     RNDERGLVRKTLEISFEEAAHGCTHQVSYERVDQCSHCSGSGGEPHSRQETCGACGGR
                     GKVRFQQALFPIAMDRPCSRCRGTGKLPSVPCSNCQGSGLGKSTRRVEVDIPAGVEDG
                     SMRVVSGAGNRIRPDRPAGDLEITVRVASHEFFRRVGDDVACRIPVSFSQATLGGEVE
                     VPTLDGSARLRIPPSTQPGSVLRLRGKGIPHRVRSGRGDQLVEVTIRVPEQLSERARR
                     LVEELGHELGETVNPQEASFMEKLKGLFS"
     gene            17530..17922
                     /locus_tag="VFQ61_29345"
     CDS             17530..17922
                     /locus_tag="VFQ61_29345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HET9958648.1"
                     /translation="MDDDERRRSPRAPIELKVEYKRLNTFFADYTKNISRGGTFIGTE
                     RPLPRGTEFVFALVVPNMAEPLRLRGKVIWTTEPHAATRANPAGMGIEFQYDSAERFA
                     KEASVERLMLQELGEHLTTRLLGRKPQT"
     gene            complement(17958..20207)
                     /locus_tag="VFQ61_29350"
     CDS             complement(17958..20207)
                     /locus_tag="VFQ61_29350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958649.1"
                     /translation="MSSGFRFCELAGLACLVVSVCSSAGCSSTTAARPTASSANASAG
                     AVNPPPLSEEEFAEKAYQILLEPQPSQDRTRLLVAVVRQQLKRAAARFERDNGNRPGL
                     SALLGAFYLVRAGEHRREFTEGADAALSAGAAEVARLGQEGFSIALYSMLATGLPSGP
                     AHDEVENHLKAILDFSRATQGSRPLQALGRAARVSSQRAMLESSTEAFNSAREQLVSW
                     MRRALESTSSEQPLRLSGDRDEALETYRAMRGGPIVLTALHLRHGDARGALHAIDNAD
                     LGRLIPPGLRDHLERAAEERDPDAWAALFRLFRSAAESTGPLTQDPEAEIDTEVMGAA
                     AFGCALELYRAEPATLHGSMPLAAELIGHGMAEVAAVLLSSSLSKNAAPEELSLALSM
                     VLNAAIAENSIGQFGAARRTYLRAQKLIELAESRAFAGKIEPSAARIHYVMAAMEASQ
                     AELDRAQPLVERSIALEPSIDAFNLLAAIARQKQQPQAALDALDRVIGLARRNNDALA
                     EVEALFSKFEILRERGPEAAAAKALDETLARTVEATRLGRPGPAQARLERLLARILEQ
                     YGDRNAIRRATDRAFEAASGDVRQLSATILDLARRALTQGDLQAARMAAQRAIDAGLP
                     AEDLVYVALWLQLLERKLNVMSDGTVEEAYSSIDDTSGWPAKLRSWARGKLSDEALLA
                     AARSSTERTEAVFYSAMRGRLAGKAESDARLREVASSPAINLIEVAIARDLIAPTSKY
                     KLPAGVAVP"
     gene            complement(20459..21598)
                     /locus_tag="VFQ61_29355"
     CDS             complement(20459..21598)
                     /locus_tag="VFQ61_29355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958650.1"
                     /translation="MRRCVALVLVSSLLSANALAAGKATKKGKKNEKPSGPSIETTAP
                     DPASKEQSDKGPYRPQGKTGPLAEKAEEKAEIEEIVHAKPRDKAVIFGDAIIGFGQAP
                     KPGPADEINDRTREATSVGFVLGGQFDFSAKFSLGLKLPITTASIDRSTPTGQTNGGS
                     DKGTVLGSPLLYAEYRHPLSELTQLPILFGVGIPVAQGDPDPATADLGASNSAQIQLV
                     ADGASGYRNPEFYGPKRLPVVVGIGVRHERNALEIHADTKLVVGFNLGSNLANDKIFL
                     GKGELHQRALAVRNVTVAGVTYDLTDKPAFWVGLDAWLVYNAVEGIKYVSSAESLSPF
                     HLVAEPRVGARFGHVRPSLGFVYPIAGRLHDTEILGIRAHLDYAF"
     gene            21820..26214
                     /locus_tag="VFQ61_29360"
     CDS             21820..26214
                     /locus_tag="VFQ61_29360"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9958651.1"
                     /translation="MVEPSDRPKPQRLADFEVIRRLGAGGMAEVFLAKRRGAEDTYKL
                     LVLKRVLPAYGSSRRFRTMFAEEAQLATRLNHPNIVQVYDFQDYGDEGQLLSMEYVEG
                     PDLRRLMKAAEAKQQRIPPYVAAYIVAEVAKGLHYAHERKDEAGAPLDIVHRDVSPQN
                     ILLSYDGAVKIADFGIASANLFREEPGVLKGKTAYMSPEQARGERVSRRTDIYSLGVV
                     FHELLTGRPLHGAAEGQELLEAVRSGHVEPPSTWVRELPPELEEVVMRALMRDPAQRF
                     QTARELSASISRTLLQHQQLIDSHVLETVIAQLITREHTSPGAVGSEIAQDSQYGPDS
                     VGQPALSSVPELSVAIPQRDDEQTGPGRPAQSLRERAGREVRHVAVLKLRLHGVDQLA
                     RSVGPAAAARAIEQLRGTLGEAAFRRGANFAWEAPEAVEPGFEVVLAARAVVGLLANP
                     SRAPADAVWLAIDVHEVLRGASEDLSVKLDVSVGIVRGIASGRRDKAGHLVNHELHEP
                     SGYLADLLEQNAPPGVSWVAGGLYRLVRRDFVWGDAPTICLPEAAARNLPENMRIYAL
                     LRPHTREEKLSEMAALASDIIGRDAELADLHAAYYGVVGNEKIHGSVGARVLYGEMGI
                     GKSALLSAFLNELPPDAQVLRVECSPALSEVPFATIAEWVRELTETRIDQNLDQARGL
                     VEAALGEYAAQEEGPEVIERMAELATGRVRAFEDEADVVRSHKLMGRGLSQFFARATL
                     DAPLVVVMDGLQWADRQSLALVSEFVRQSEALPVLVLLATRPDDRVLSELEGMVRVEL
                     KGLGPEQQIRLLQARLGVTDGVLTVCAELLPRAAGNPFFLLEMVDALLERGQIEIREV
                     EPGRQELVQVERSGDFGLMLPSTLEQLIADRLNELPDEEHMIIDWLAVAGGPLSLKDL
                     DAASGLENADAAMRLCARGLCDLKTELIDVRHPLTRDVAYRAIERRRRVRMHRALGEH
                     LANTPLSRGLTAAVVARHLARGNDRAKAARFYLESAQAARASYQTRLARRYFRRAISL
                     LPEDDLLLLEAHEAMEAICRNQGRFSERRQHLMRLRKLSRASQRAHWVATALISTAQF
                     ELDGARLAKALASAQRAEVVAERAGSPLLLVQARMLIAEVLHDIGDMQGALASADRAG
                     QAAEQDSVPARLRAEVLRLRGTLLRRVGRVHEAIDAQAEAVAVFRRVGARRLEARAKN
                     SLAYGLLVLGRFEDGIILALDAIRIDLSIGGRFQIAKTLNTIGECYASLGDFDRGIAY
                     MRRARDAHERYGDQDSRADTLLSLSEALIEIGDASAAEPLVGDAGALTAVTGSAYDSV
                     HEKILRAVLARLAHDPARAVMHAFDARQAAEAQAYVAFHFYAMAVEACARVEMGEQHT
                     GILLATTAMGAIETLQGSEYGLHTRALCFRALRSAGSPQAAEAERRATAYVQRLLEQI
                     RDPAFRRTFRQRPLVREILGDVKESTGN"
     gene            complement(26271..28034)
                     /locus_tag="VFQ61_29365"
     CDS             complement(26271..28034)
                     /locus_tag="VFQ61_29365"
                     /inference="COORDINATES: protein motif:HMM:NF019271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EGF domain-containing protein"
                     /protein_id="HET9958652.1"
                     /translation="MTRSYGRVSARSWHFRTTQLAMAAGFAMGLSSACNLLLGNEPGS
                     RDDSAKGGDSSQGGSSGSNVGGTNGGKPQSSGGQTASGGLEGVAEGGDGQSGGSTSTG
                     GSTSTPEPVCEPECGQYAECQWDGANAVCACLEGFAASKDGTCFDIDECSVENGGCDA
                     TAVCINFPGGRDCDCQAGYELDPENDRRCVSSCESAGCDPHATCAAVDGEVTCTCASP
                     YVGDGKTCTFDSGCEAKNCDENAFCDPAKDNQCVCAAGFTGDGQTCTNIDDCAAAPCK
                     NGGQCKDGIGSFSCDCSSTGFKGKTCEENIDDCAANPCLHGTCVDGLKSYTCKCEAGY
                     TGTNCETNVNDCAASPCVRGTCSDLVNDYKCTCPTGYGGKNCETQISGCVSKPCVRGT
                     CTDLTNGAYKCTCPAGYDGVNCENNIDECKSKPCVHGTCTDKVNGYQCACSAGWGGTR
                     CDTGNCANMTCPASAPCRTSSVSGKVAVCFPTACKAAAGSGGLCLAEGTNGSGAAKLL
                     KGVNGNFADLDWANRAKYFGYIDYRVNNKARVCVFSGTNYSGTRIVVPHGEVRTTSSG
                     FGRSNGWPAGSNETVDYGCND"
     gene            28236..>28485
                     /locus_tag="VFQ61_29370"
     CDS             28236..>28485
                     /locus_tag="VFQ61_29370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958653.1"
                     /translation="MQPLRSVGLGLGGVGLGLVGLGLVAITVRLGWANFVAGHVPAPP
                     VGASALSSGLPNVLALRSDSGAAKGTDDRSNHPQSLLPR"
BASE COUNT         4872 a         8878 c         9371 g         5364 t
ORIGIN      
        1 gccgcgctcc tcgcgccact cctcgcgcca tcggatctgg tcgttctgtc tgggccgctc
       61 ggttcgggca agacgttcct atgccgtgcc ttggcgcgcg cgctaggggt cacgggtcgc
      121 gtgacgagcc cgacgttcgg cttgatccac gagctcgaag gcagccagcg catcgtgcat
      181 gccgacttgt accggctgaa gagcgaagcg gaactagtgc acctcgggct ggatgccttg
      241 cgcgacgaag ggcggctgct gctcgtcgaa tggggtgaag attacgcgcg tgcactcggc
      301 ggggacgcac tttacgtgcg attggctctc gatccgcgtc gcgctacgtg cgtcgcgagc
      361 ggggcgcggt ccgagcagct cgtcgaacaa ctcagtgcgc gcgccgcggc cgaggccacg
      421 gtcgattccg gagctcgtga tggctgaagc cgaagaagtc acgccgagcg caagcgccgg
      481 ctggatcaac ctactcgcga gccccgtgct gcgtttgctc gagcaggtgg gcatgaccgt
      541 cgagctcact gggcgtacgc tggtttggct gattcggccg ccgtatcgcc tcaatctgct
      601 gttttcggcg atggacttcg tcggcgtgca gtccatcttc atcgtctccc tcaccgggat
      661 tttcagcggc atggtgctcg cgctccaaac cacgcacagc ctgcgtcagt tcggggccga
      721 aggcgtggtg ggctcgctcg tggcgatctc tctgacgcgc gaaatcagcc ccgtgttcgc
      781 ggcgctgatg gtcaccgcgc gagcgggtag cgccatggca gccgagctcg gcaacatgcg
      841 ggtaaccgag caaatcgacg cgctcgtgac catgggtgtc agccccattc agtatctact
      901 cacgcctcgg ctcgcggcgg cgaccgtcat gctccccttg ctctgcgtgc tgtacacgtg
      961 cgtgggcatg gttggagctt gggtggtcgc ggtgcaatgg ctcggagtcg atccaggcac
     1021 gttcttgtcg aacatcgaga agtacttcgt gcccaaggac ttcgtgatgg gcgaaatcaa
     1081 ggccgctgtc tttggctttc tgatctccgc cgtggcttgc agtcagggct tccacgcttc
     1141 gggcggcgcc aagggcgtcg gggaggccac cacgcgcgcc gtggtccaga gcgcggtagc
     1201 gattctgatc gccaattaca tcttgaccag ctggcttacg gatatgtgag cgctcgaagc
     1261 gaactgaacc gcagaccgtg agcacggcgg tgttctgcgc tattccgatc tttcgatgcc
     1321 ttgtcctgga aggcgaccga acgtttgccg cgctagcggc aacaagcttt gacatgcctc
     1381 gagtcccaga tcggtgtgct ccccagggtc ggtcgcgaca tcgaagcaac ggtatccttg
     1441 atcccaggaa cgcgcttcca gcttcaatga gccgcgcata tagccccagt tctcgaaggc
     1501 gcagctccat actccggcgc agttcgtcat cggcagggcc gtatcggtca actcgggccg
     1561 cagtaggctg tgccccggca agcgcgcgcg gtactccgcg atctgcggcg cgtccacgac
     1621 ccccatcaag tccagcaccg tagggaccag gtccacgtgg aaggtgtagg cgtcgcgctt
     1681 gcctcgtagc gcggctcgct cgggctcggt cagcgtgcct tcgggcgcgt cgatccaggc
     1741 gggcacatga atctcctcgt cgaagatgct gaacgtgtgt cccatctgac cgtgctcgcg
     1801 gaacgcttcg ccgtggtcgc tggtgtagac tacgaccgtc cgcggccccg ccgcgctctg
     1861 cttgatgtgg ctcagaaact cggccacgtg caggtcctgc tggtagacgg agttctgata
     1921 gtagttcttg aaatacggat tgtcctcggg ggccttgctg gtcgtccatg gttcgaaggg
     1981 cgccggcaga ttctcgtcca cgaaataagg atagtggacg ttggagagct ggatgacggc
     2041 caaaaacggc tccttcagct gcgcgaactg agctttgacg tgccgggcca acaagccttc
     2101 cggtgcgccc atgtcgaggt ccgaagtggg ttctagatcg gtggcgctcg cgaagttcga
     2161 gaccccgaga ttttttaccc acaaccgcgc attgccaaac atcatatttt ggctcgtcca
     2221 aaatgcggtg tcgtagccgg ccgcgcgcgc gtagtcgaac accagtggcc aggtatgcag
     2281 cacctctcgc gactcggtcg ggcgaacacc agaccagagc accgcgagcg agatcgccgt
     2341 gcacgagtcc atcgatctca tctgcgtgag cgggaaccgg tcggggaaca gcagattcgt
     2401 cgagtgcgtc ttgcggcagt ctggatcggg ttcgatgcac acactgtccg cccgcacgct
     2461 ctccaaaata acgaacacga cgttgcgcgg cggcgcttcg gccgcgcgcg ccaacggggg
     2521 cacgggcaat gatgcccggg cgcgggggcg aacttggttg gactcgtccg tcaacccgaa
     2581 ttgagtgcgc agcaggcctc caacggcatg caagtagagc acgtcgggcg tagaggcctg
     2641 cgcgtgtcga tgttgagtgg ggatgaagaa gctgccaatc aacagcaggg gagccagcgc
     2701 gccaacgatc agcgcgaagc ggcgacgcgg cctcaacagg cggcgggcaa accagagcag
     2761 cagcagcgcg ccgatgaacg gaggcagctt ggcgcgcgcg tagtttccga tgtctgcaaa
     2821 cagctggttg agcacgctgt ctttgaagtt cgaggcaaag acagatacat ccacattgag
     2881 gtaggcattg tactgctgaa aaaagtagct ctgcccgccc atcgtgaatg tgaacgcgat
     2941 cacgaacagc acggagttca gccagcgcag ccgaccccgg cgccgcgagg cggcgtatac
     3001 cagcgcagcc cacaccagca tgctttcaac gacggcggca gagtaggtca acacgtactc
     3061 tggttcgaag gctcggatcc gcggcccgcg tcgcagatag tcgaggctca ccacgctgat
     3121 tgacggtagc agtaggagcg cgccggccag caggcgccgc gtgcgcagag acggaaagag
     3181 ccgcggtgtt cccggcgcga ccgcggttgg ctgcgcgtgg agagcactcg ccacgcgaga
     3241 cgccgcgtcc ccattggcgg atggccttgg caattcaact ttctcgctgg gctcgcggcg
     3301 gagaacctcc ccatccgcag gtcgactagg aaccggtagc acggcgccca ttctagtctg
     3361 ttccggcgtc gcacacgcaa ctcctcgcat cccggcggag cgcatgcggg aaatccgtgg
     3421 ggcccggtcg cgcggctata ggctcgggtt cagcggccgt actcgggatc gtggcacgct
     3481 ccagtatctc gctcttgttc attctgctgg caaccttcgg gtgtaagacg acggagacgg
     3541 cccagcctga gaagccttcg gccaaggcca aaagtacggc caagcccacg tcggtcaaag
     3601 ccgcatccag caagtcgaag gcgaaggaac cggagcacgc cgccgacgag aaggccgatg
     3661 cgcacaaggc cgacgcacat aagtcggagg cccacgagcg acccgaactg ccgcaaaagt
     3721 acgtggttcc gttcgcgtgg gaggcatctc gtgatgaacc gttgtcccag gctcgcggat
     3781 tcctgaagga gatcctgggc gataatcgga tctacatgga gcacgggtcg gccttctttg
     3841 cggccttcgc ggaagctcag aagccacggg ccacggtggt gacctgttct gattcgcgtg
     3901 tacataccac ggccttcgac gtcacccccg agaacgacga cttcatgatt cgcaacattg
     3961 gcaatcagct cgcgaacggg cttggctccg tcgagtacgg cgtggagcac ctgcagacgc
     4021 cggttctgat ggtcctggga cacaccggct gcggcgccgt aaaggccgcg atgggagata
     4081 tctctgggct ggcggagccc attcgccacg agctcgagcc gctgcacgtg agtcgagcga
     4141 aagccggcgc aacgcccgag gcagcctggg ccgaggcggt agtcgaaaac gtgcgatcgc
     4201 aggtgtcgtt cgccctggag cacttcggtg agcgcgtcca gcagggcaag ctgaccgtgg
     4261 tcggcgctgt ttacgacttc cgaaatgact tgggcaacgg agccggcaaa ctcacggtcg
     4321 tagacgtcaa cggcaactcc gagtcggaac gcatggaggc gttcgttcag gccgtcgaaa
     4381 cggatccaat gaccggtaag aagggtgccc gccgtagcgg gcgaaaggcc gatgtagagt
     4441 ctcccgagat gagcactgcg ctcaagcgcg tgattctgca ggctcgagag atgcagtccg
     4501 acggcaaagc cgcgggtgcc gcaacggaac caaagaatct acctgcgaaa ctccattctg
     4561 caaacgaaca ctgagacgga ggcgcgagca ggttcaacgg cggcaggcca actgcaagct
     4621 cgctcgattc tcgacgataa ctcaacggtg ttgcagaacg agactcggaa gatcgagcga
     4681 agtgcggttt ttgagctcga tctggctccg ggcgagccgc gcgcggtctc caaaattttg
     4741 ccaaagtggc ttgcgcgaac gcgctagaca tagcaaaccg ttggccacgt tgacgccata
     4801 cccgaagggg tgaggcgcga caccgaagct ccgccgccga cctcgagtct gctgcttcgg
     4861 ggcttcacta ccggcagacc gcattgctct tgggactgct ctgctggtag ggtcgatgaa
     4921 cttcattcgg agcgcgtcgg cccgttcgga agtgcgaacg aggcgtcgct tccgaaagca
     4981 gctggcaatc ttctcgctac tgaccagaca ctcgaatcgc gagagactcg agcttgaatt
     5041 tgtaagctcg agaagagccg aagtgatggt acgcaaacca tcagggttgt ccgaaagcgc
     5101 tgtttggcgc gcgcgtggtt gtttgcggct gttttctggt ccgtggctgt tttctggtcc
     5161 gtggatcggt ccacggatcg gtccacggat cgggtccacg gatcggagcc gctcaatgtt
     5221 ggacggctcg cagttggagc tccgaagcgc agcggcttcg aagcctcgaa gatttgaaca
     5281 ctcgactaca ggccgcgcgc tcactctaac ggaaataact atggcaagtg caaaagcagt
     5341 ccggggaatg gtgttggtcg gcggggttac atatcggatc gtgcgcctgg ccgcgggctc
     5401 ctattccgtc atccgcattc tcgacgatgt cgaggtgggt cggttcgagt gcgcgcccaa
     5461 actgagggtg gagcgaacct gtatcgatcc tgtggagttc ttggtgattg cccgtgaggc
     5521 catccaaacg gcgaagacga gctgggtcgg ccgcgcggtt gtggaccccg tagcggcgga
     5581 gcgccgcttc gagttgccgt cccgtccagc gaactgagtt cgagctgcac aggcgcgggt
     5641 catgggccgt cagttctttg tcgccgctga gggagcgaac gaaagggacg ccgaaccgcc
     5701 agaagtttgg cccaaagacg tcggcgagac tggccaaact gaggtttggc atatgcgagc
     5761 ggccgttcta tccgacgctc atgcacccaa cgccttcgag cgattcctct ccagaccttg
     5821 cactcttgaa atagccattt cagttttgca ctgcgcacgc gtcgaaggca ctggcacttt
     5881 gctgcggtct cgccccgctg cctcgaatgc gtcgcttcag ccccggcagt tgggacgttt
     5941 cgggtatgta tctcggctgg ccttccgacc ttcggagatg cattggcgtg gagcaccgtt
     6001 tagcgctagc gagtgaagag atcgcccatg accgagaccc ctcagggcac ggagcgcgtg
     6061 atcgacggta cgcgccgcgt tttttacgat ggctactgga ttaaagcgta tgacgcgcca
     6121 gcggacacgc ttctggccaa gaagcgtctc atcgaggctc tgacccgccg tctgttcaat
     6181 cataccgagc acggcatcaa catccccgga acgcggctcg acgaagcgcg ccgcgccttc
     6241 gattcggaga cagacccgcg tcgtaagcgc gtcaaggggg ccatgctcgc gggagctttg
     6301 ttcaaccgcg ccacggacat cttcaccaag ctggtggaga ttcaggcgct cggcgtcgag
     6361 atccagtctg acaacgcgct gatgcgacgc tgcggcgagc acctgcagga ggcgctcggc
     6421 ttgggcaaac tcgttctaca ccgaagtgga gacgaaggta tcgacgagct gtggggcgag
     6481 ccgttcaaag ctttcgcatt cccgatcgag gagttctaca agagtcgcta catcaagatg
     6541 gcgctgacca tgcgcgccat cgatcagatc gcggatgagc tgtgcctgac cttcgccaac
     6601 ttccccgaat tcgcgggcgt aacggagttg gtccgtgagt ttgcgcgagc ttcgaagttg
     6661 aagtgcgaga cgttgcggac ggacaaggac atcttcgacg tttggacgtc tttcgcggtc
     6721 tcgggagaac ggctttgcgc tttcgctccc caactcccca gcgacccgaa caacgacgtg
     6781 atgcggcgcc gcctgaagaa gggcctgcgc ttgctgcact ccggtaagga gctggtctgt
     6841 tacatcgcgc gtgcgcgcgt gccgatgccg aagagtacac acgccctgat cgagcgttgc
     6901 attcgctacc ggaacgaaat ggacagccct cgacgcagcc gcccctcgga acggcccgcc
     6961 gcgcgcacgc tttctcgctg attggctgag cgctcgcccg ccttgggctc aagcagctct
     7021 cggtgactca actcagggcg agcatacggt cgatcggaat cttggcgcgc tcgcgcagct
     7081 ccaccggcat ttccaggcgc ggctccagtt tgtcgagagc ttcgtagacc ttctccggag
     7141 tgttcaggcg catgaacggg cactcgttac aagcgcaatt ggcatccggc ggagccggga
     7201 tcagctgctt gtggggcgcc cgcttctgca tttgatgcag gatcccgggc tccgtcacga
     7261 cgatgaacgt ctgggcgggt tgagtctcca cgtacttcaa gagcgccgcc gtcgagccaa
     7321 tgaaatgcgc tctatccagc agcggctctt cgcactcggg gtgtgcgatc accagggcat
     7381 cgggatgctc gtgcatcaac atctccagct tcttgaggct gaaggtctcg tgcacgatgc
     7441 agctgccttg ccacaacttc atgtcccgac ccgtcttctt gattagataa cggcccaggt
     7501 tcttgtcggg tgcaaacaag atttgcttgt cggcaggtac ggcgcgcacg atcttttccg
     7561 cgttgctcga ggtgcagatg taatcgctct cggctttcac ctcggcgctg cagttgatat
     7621 agctaatcac caccgacccg ggataacgtg ccttccaagc gcgaaagcga tccacggggc
     7681 aaccgtccgc gagagaacaa ccggccttca tgtccggcac caacacgatc ttgtccgggt
     7741 tgaggatctt ggccgtttcc gccatgaagt gcacgcccgc gaacaggatg acatcggcct
     7801 gtgtgccctt ggcggcctgc gcgagctgta gcgagtcccc gatgaaatcc gccaagtcct
     7861 ggatctcgct ctcttgatag tagtgagcga gaaggacggc gttccgttcg cgtttgaggc
     7921 ggcggatttc ctcgtccaga tcgagcgatt gagagatgct ttgggcggtg ctggggtttg
     7981 ccatcttccg actacttcat ccggctactt cgaggcgcct cggcgctcgg tccgagaagc
     8041 ttggctcagc gctcgtgctt tcgctagccg ccgctcgggg cgttttgctg gtcaatcgtg
     8101 ggtacggcag cttggcagcg ttcgcggaag acgtcgggcg acagcccttt gctcagatcg
     8161 agctcgaagt cgggcacgct gtgtgcggca agtttgtctc gtagctctcg gatttcgcgc
     8221 cgccccagcc ctagctgctc gagggccgct tgatcgagcc gagcgccggg cttgccagat
     8281 agagcctcgt cttccagctg cacgagtttg cgtagggctt cgagctccat ccggctcagc
     8341 cgcgaaccgc acacgtcgta atcgatccac gcctcgtaac tcatgggcgc gatgcgcttc
     8401 agcatcccag ccatcactcg gccaaactcc tggatttccc actgagcatg gggatctacg
     8461 cgcagtctca agaagtgaag caggttgtgc agatcgatct tccaatacca ttgagtgtac
     8521 gtcgagagcg gcaagtcgat gcgcgatagc tcccgcgcta cctgctcgcc tgccagccac
     8581 tcgtacgtat cgcggctcag tcggcgaatc tcttcccagc gcttgcgcgc ctcggcgtac
     8641 gtggcactcg gcgcggcctg gccgctccgg ccttgattgt tggtgcggct ctgctgctgg
     8701 aactcgctcg cttcgggcgt gtagaacagc atcggcatca acgaataccg gcccgaatac
     8761 tcgttcacgt tggcggtgcg gtgccgaatc cattgccgcg ctatgaacat cggcatggag
     8821 cagtggaact tgagttccac catctcgctg ggcgtggtgt gatagtgccg ccgcaggtag
     8881 cgcaggagcc cgcgcgtttg gctcgttttg cgtgttccgt agccatagct cacgcgcgcg
     8941 gcccgctcga tgcattcgtc cgtgcccatg tagtccacga gcgcgacgaa gccgtggtct
     9001 aaaacgggaa aatagcggcc gagcagctgt tctgcgcctt ccgagacggg acgttgcgtt
     9061 tcactcattt gcggcagctt ctcctggctg ggcgagcgct taccactcgt tggcggcccc
     9121 ggctactgcc gcgctcgttc agcagccgag ctggcgcttg ctgcagctca gccgaactgg
     9181 tgccggtcgc gccgcactgc gcgtcgcggc gtaagctgct cgtacgctcg tacggcgtgc
     9241 ttggcggagc ggagtgcgcc gggcagggaa gacacatccc cgcggctgtc gctgtgcgcg
     9301 aacaggatgc gcccgtggtg gcgggcggca atatccgcgc gtgaaagctg acccggggtg
     9361 gggatagcca aagcgtgtcc tcgtcgaaac acccgtaatt ccgcggtcgt tcttgcgagc
     9421 ccagggaaat ggcgatcgag gtgggtcagc gtttgctcgg tcaacgccat cagcgtttga
     9481 tcgtcgagca aatcgcgacg ctgggcttcg ggcaatgggt gataaatcgt gagcacactc
     9541 ttttcgtgct tctctccccg accggcacgc gtgacccaat cggcggggat gaagtccgaa
     9601 aagggcgtgt ccaagaacca gttgtcgtag gccggctcgg gcccgcgctg attcaggcac
     9661 acgttgatca cagcataagg cgcgtagcga aaggcctgca tggcttcgcg ttgctcggcc
     9721 gcgagctcgg gcactagccg ggcggctaga aacttgggga tggcaagaat cacggcgcgg
     9781 gcccgtaaga gcacaggctc ccccgcgctg atcgcgtgga cgtgcacttc atcgcccgca
     9841 gtctcgatgt gatgtacgaa cgagttctgt cgcagtcgag ggcccaactc ctggtgtacg
     9901 gctcgtgaca gtgctcgtgc tgcaacaccc agcccacccg gataggttag gcggcgctcg
     9961 gcgccaccca accaaccgta cgcttgcacg ccgagtcgca tcgaagtgtg ctctggtgcc
    10021 ccgccccaat tggaagccgc atagcgttgc cagaattgct tgagttcggg gccgtagggc
    10081 gccaagaaat cggcgaagcg cagggtatcc agctcgtggc tgtcgcgagc ttcccgcagc
    10141 cggacggcct ccgcgaacga gcgctgaaaa tcatcgcgtg cacgttgcgg cagcggcagt
    10201 cgtggggcac ctgcgccgaa gaagtcgaag tacggctggc ccttcacaat cagcgcgtct
    10261 ccgccctcga ttttttggcc gggggcaccc accgattgca gcagctccac gagctcggtg
    10321 tctccctcgc tgaagaaggc agcgccggtc gaaaacggaa ctccttccca agtgtccgtc
    10381 gaacaattgc cgccgtagcg ggactctttc tcgagcagaa tgacgtcgcg cttggcgagc
    10441 aagtgagcgg cacacagacc actcggaccg cccccgacga tcacgacctc gcacttttcc
    10501 gagcgttcag ccttgggaaa ccggtgtccg tcacgcttgg cgtggccgtg cgtgaagtct
    10561 tcaccgtgca cgcgcggctc agcgaccgca tgtgcggcgt gcagtccggg ctccaaatgc
    10621 gcagcactgg cagccgcatc cgcgcggctc ggctcgggcc gactgtcggc gtgtttgcag
    10681 cccgatccca agcccaagct gccagagatc acgactttca ggcaatctcg ccgggtgaag
    10741 tagattcgtg tgtcttccaa cgcgcgcaca tagtgcgcgg attcgctggt cgcgcaaggt
    10801 gaacccggca gttacccggt ggacttacgc agtcctgcag tcagagctgt tttttcgacc
    10861 attctcggcg gcctgcgtgg catccatgaa tctcggaagg aggcgtgggc tggcccctcg
    10921 cgtgccggcg ggacaaccat ctccggctcg agactcggca cgaggctttt ggcgcaaaca
    10981 tgggaatcga ttcgaggcat cgagttgatg tggcgaatct ctatcggact ccactcacgc
    11041 caatcaccgc ccgatacacg tgctcggccg tgcgaagctt gcccccagta gaaccgtcga
    11101 atttcaaagc gcgcccgaga tcaccggcat gggtcaccga tcggctgcgc cagcgcgcct
    11161 tgcgtgacga gaaccgccta catggggaca agctgtgggg tgactctgga gaagtggccc
    11221 cgagtgatcc tccacgcgat atcacgcaga aagtggccaa aagctcagtg tgtgacgttg
    11281 ggccgagctc atgcccaggc tcacgcgggg catacgtact tacaacatct cagctatttc
    11341 ggcgggaaac acaggatttc ggtgccaagg tcgagtccgt gcattagctt ctcaacatgg
    11401 gcacatcgag acgaaagctc ggaacggcgc tcgtcgcgtt gggtgcgggg tgtctcgttg
    11461 ggatggcggg ctcttggctg gatttgcagc taagccagca tctgcaaccg gttccgctcg
    11521 cggcgcaggc cgcctgtgat cacgcgccgt gctcgcctcg agcagctttc ggacaagccc
    11581 tgcgtatcga tgcgctgggg aacacctccg attcggcttc tgcggatcag cttggggccg
    11641 tgaccggcgg cacagagccg gattctcagg tcatgagtgc tccaggcgca cacctggcga
    11701 agcgctcgcg cttggtgaga tcgcggggct ggccgttgct cgtcggcgaa gccatcgatc
    11761 cctgggaaga tggcgccgtt gcgttggctt cggctcaatt tcgtcggcgc acggtgcgga
    11821 gttcgtctcg tgaggtgtcc tgggcgtatc gacgcgacgt cgtagatccc tgggccggcc
    11881 cgaccttgcg ctgattcgcc gactgagcag ccaacggccg ctaacgcctt tgggcgcatt
    11941 gaatctggcg cgcgcacggc ggacttggtc caagctctcg cgcaaatgaa ggttctcccg
    12001 cagaagctgc ctgaagtacc tcccagaact cagtggcgtt ccggaagcaa ccggaagtag
    12061 accgcggagc ggtggaagtc ccaccatcga tgtggaacgc ttcgggcttg aggcgtacgc
    12121 gattgaacgg cagtgaatgg tctcacggga gcaggaacca agcccgaatt gagcaggccg
    12181 ctcgccgcgc actgcggctg acggtgggct ggggggcttt ggccggagtt ctgttcagcc
    12241 cgtgcgcgtt ggccaaatct gctgctgcgc cggccaggct ctttccgccg agtgagggcc
    12301 ccgacgtgaa cgtgctcggc gtggagaggg acgggacccg gcgcctagtc gtgctgggtt
    12361 tgcgggcgct ggctcaccca agcggcgcga tcgaggtcgc gagcgagttc ctgcctacgg
    12421 cgaaggccgt tcaagtgctg agtctgcccg agcggctcgg gggcggcttc ctgttttggg
    12481 cggtggcctc acgcaagact cagctgtgga gagccgaaag cttcaccggc actctcgagc
    12541 ctttcgcaga gctcgatttc gaagcggaga gactggtttc gggcttcgat cgtctttacg
    12601 tgcgttcacg caccagctcc gagtggttcg cgctcgacgc ggagtcgggt gccggcttgg
    12661 ggcgaggcaa tctcccgacc tcgcccagcc acggggagat ggcgttcgcg gacgagtggc
    12721 tggcggcggt agaggtgcct gcgcgcggtg tgctcgtgag tttcgacgcg ggtggcacct
    12781 ggcatccgtt ggggcgtcca ttcgcgatcg aaggagagct cgaaggtggc gtcattctga
    12841 gcttgggccg tcagcgttat cgcttgagcg ccagcgggag cctgttgccg gagccgagca
    12901 caccgacgcc tccacgagag ctcacgccct cgttgccaaa tcggttcgcg gggctcggct
    12961 cgcccctcgc ggccgcgctg ttacgcggct atgcggcccc ggctagcgcg gggccccgga
    13021 ccgcgcgtga ggcctgggtt gctttgcgcg gcgctttggc gcgcgtggat ctgggtgatg
    13081 ggcgtgtgat cgagacgctc gagcgccccg aattgcggga cagcgaatgc acggctttga
    13141 ccctgggcac aggcttcggc ttcgtttgcc agggcgcgcg tggggaaacg cgggtgcttg
    13201 cggcgcgtaa agacatggcg ctcgagctcg tgcagagctt tccaactgca cggagtgtga
    13261 tctcgagcgg caacggtggc ctggtttggc gcggggaatg cgcgcccagc gccaactcga
    13321 cggcagccgc cggggtctac tgcgtgcgtg ctccgagcgg atcgagtttc gaagtggttc
    13381 tgcgcggcga tcgcgaaacg gcgcgcgtgc tggccctctc ggacgggaga gcgggggtat
    13441 tggtcacgcc tcagcctggc cgcaaaggtt cgctgagccg ggtggacgcg cacggcaaag
    13501 cgcgaacact gccgctgaag ttacttggcg acgaccccaa ggcgcgtgaa ctcttcgagt
    13561 ccgggttttg gctcgagacc tgggtcgagc gcgtcgacgg gcaactctcg ggctgggtga
    13621 ccaatcgcgg ttcgttcgcg ggtgtacgcg tgctgccgga cggcggcgtg aagattgggc
    13681 cgtcacgccg aggcatcgag cgtgcgctgc tgtcggggga gcgtgcgctt tgggtgcccg
    13741 gcaccggcgg cgccgaagag accagggatg ggggaatgag ctatcacgac gtggatctac
    13801 cgctggagct cactgcggag agcgccaagg cggcaactct atcggggctc gagcaaggct
    13861 gctccctgct cggttgcgtt tacgccggct gggcccatgt gggcgctgga ggttcagagg
    13921 acccgccgca tctcggggtc gcctcgtccc ccaagagcac tcgtttcccg cagcctggcg
    13981 gagggcgctg gatcttgcgt tgcagcctca cgggggatgc atcccgggtc gtgccggctg
    14041 ctgcccaaag cacctcggag gagagtggag ccccgacttg gggaccattc ttggaatccc
    14101 cgcctcccgc gcgagcgcag gacgtagtcg gcttcaatgt ttccggcgaa ggtggcgcga
    14161 tgcgcgccta cgcttgggcg gccaaaggag cagacttcaa gaaggctggg cgcttcgcgg
    14221 tcagcgtctt ggatcgctac cgcacgacgc agggtgtatg gagcaccgcg ttcgcgccga
    14281 gcccgtggtc ggatgtggcg caggtttcgg agttgttcgg ttatgacggg gccacgccga
    14341 gcgcctggcg ggtgctgttg gatcccggcg gtcgagcagg tgtgttgagc gtcagtgtgc
    14401 gcggcaacac agacctgttt gcagtggaag agaacctggc tcccacatac ctggggagcg
    14461 gctctcgaca gggaatcacg tcactcagtt ccgctgtaaa actgggcacc atttattacg
    14521 tagctactta cgaggatccg cggaccttgc gtatcttcca agtctccgag gggcgaacga
    14581 agctcgtcgg ccgatacacg gatctcccag aggggcgtgg cgtttcgatg tctctggtgc
    14641 ggagtgccgc cggcgatgcg cttggacttt gggcccgtag cggtgccggc tggtacgtat
    14701 tcccgatcga tttggcgacg ggccgagcgg gggcgccgac ggaagtcgat tccgccagct
    14761 tgagtaaact cccgcgctct tgcgccgtgg acgaggaggg ctatctgctt gacgggccgg
    14821 tcggggtgga accttacgtg gacttcgtag gcgccgcaga tcgcatgtcg gcgcatggtt
    14881 acgtcggtcg attcatcctg ggccaacgcg atatttgttt gagtgaactc tctgctatcg
    14941 cggatggcgc cgttgaccgg cggctcacgc cagaagccaa gagcgctcca gagcagcaga
    15001 tgcgcgtccc catgacggtc gtcgaccgca ccgaacacgg ccgtaggtgg ggatttcgct
    15061 gcggcagttg agagagttgc agcccgcgtt cgaagtccga tttaggacgg cgctgcgcgc
    15121 tgttcgttct gtctcaatac gagtgggtcc gggcggacac cagttcaccc gtccggtagg
    15181 tccgccccgc acccaacaaa attacgcttg ctcccccaag gcgattgctt tccggcagac
    15241 cgactgctac caatccgttc gtccgcgtga agcgaggctg tccccccccc cggccccttc
    15301 acgcggacgc ttattttttg tctgggggtg gtgtcgaagc gcgagcgccc agcagtcgtt
    15361 ggctcatgat ctcgccgagt tcttgcgcac gtcgcgttgc cgtatcgacc gcgtcgatca
    15421 acgttttgcg tagagctccg gattccaaag tgtggagtcc cgcgatggcg gtgccgccgg
    15481 gactggtgac catgtccttg aggcgcccgg gatgttcgcc ggtgtcgagt aacagcttcg
    15541 cggagccaaa tacggtttgc gcggcgagca gcagtgccgt gtctcgatgc aaacccactt
    15601 ttacaccgcc gtccgcgagc gcctcgataa tcaacatcac gtaagcaggg ccgctaccgg
    15661 aaagtccggt cacggcatcc atcaagttct cgtccaatac gacagagcga cccgtcgcgt
    15721 cgaacagcgt gcgcgtgatg gccacgtctt cgggcgtggc gcgggtgccg ggtgcgatgc
    15781 cggtcgcacc cgcgagcacg attgcggcgg tgttcggcat ggcgcgaatg acccgcaccg
    15841 tactggggag ccgctgctcg agcgcgcgca ggggtacgcc cgccgcaacg gacaccacca
    15901 acgtacccgg acgaaagtgc tcacccagct gatcgagcac acgatcgatg acctgaggtt
    15961 ttacggcgag gaccaccaag ttcgcccatt ggatgagctc gcgattgtcc gtatgcgtct
    16021 tgatgccgta ttcgctctcg agctccgcga gccgctccgc gcgcacgtcg ctcgcccgga
    16081 tttgttcggg ggatacctgc cggctcgcga gcatgccgcg aatcagggct ccggccatgt
    16141 tccccgcgcc caacatcgca atcttggtcg tctccatctg tcctcgttct cgcgtgttta
    16201 tggttgagcc ccacgctggg ctggaaatga acgcccctcc gcggtgcacg tcggcgtgcg
    16261 ccctaactga atagaccctt gagcttctcc atgaagctgg cctcctgcgg gttcacggtc
    16321 tccccgagct cgtgcccgag ctcctccacc aggcgccgag cacgctcact gagttgctct
    16381 ggcactcgga tcgtcacctc gaccagttga tcgccgcggc cgctgcgtac tcggtgcggg
    16441 atgcccttgc cgcgcaggcg caaaacgctg cccggctgcg tgctgggcgg gattcgcagg
    16501 cgcgccgagc cgtccaaggt ggggacttcc acttcacccc cgagggtagc ttggctgaag
    16561 ctgacgggga tccggcacgc tacgtcatca cctactcgcc ggaagaactc gtggctcgct
    16621 acgcgcaccg taatctccag gtcacccgcg gggcgatctg gtcggatacg attgcccgcg
    16681 ccgctcacga cgcgcatgct gccgtcctcg accccggcgg gaatgtctac ctcgacccgg
    16741 cgcgtagact tcccgaggcc gctgccttgg caattcgagc acggcacgct cggcaatttg
    16801 ccggtgccac ggcaacgaga gcaggggcga tccatggcga tcgggaacag cgcctgttga
    16861 aagcgcacct tgccgcgtcc gccgcacgcg ccgcaggttt cttggcggga gtgcggctct
    16921 ccgccgctgc cgctgcagtg cgagcattgg tccacgcgct cgtagctgac ctggtgagtg
    16981 cagccgtgag ccgcttcttc gaagctgatt tccagcgtct ttctgaccag cccacgttcg
    17041 tcgttgcgga acccgaacgc gcccagcaag tccccgaata gatcttcgat cccggagaag
    17101 tcgaaatcgg tggccacgcc accggcgcct ccgggtcgga acgcgctctc cccgtaccgg
    17161 tcgtacgcag agcgcttttg tgggtcactc aggatctgat atgcggcgtt cagctccttg
    17221 aagcgctgcg ccgcgtcggg gtcattgggg tttcgatcgg ggtgatgctg gatcgcgagt
    17281 ttgcggaagg ccgccttgat ctccgcctcg ctggccgagc gagggatccc gaggatttca
    17341 tacggatcac gcacttccct gggtgcatag caccggggcg caaatgtgcg ctggttttta
    17401 tggagaccgg gcgctccact tgcccgcgcc cagattccag cgggctacct ggtgcgagcc
    17461 tttcacgggg ggcggtgtta cggcggtgtt cagcagggac gggctgggct aaagatcgag
    17521 tgtgctggca tggacgacga cgagcgacgc cggtctcccc gcgctccgat cgagctcaag
    17581 gtcgagtaca agcgcctaaa cacgttcttc gcggactata cgaagaatat ctcccgaggc
    17641 gggacgttca tcggaaccga gcggccgttg ccccgtggca cggagttcgt cttcgcgctg
    17701 gtcgttccga acatggcgga gccgctccgg ctacgtggca aggtgatttg gaccacggag
    17761 cctcacgccg cgacgcgcgc caatcccgcc ggcatgggca tcgagttcca atacgactcc
    17821 gccgagcgct tcgcgaagga ggccagtgtg gagcgcttga tgctccagga gctcggggaa
    17881 cacctgacga cgcgcctgct aggtagaaaa ccgcagacgt gagccgtcgt cctagcgcgc
    17941 ccgcgcgggg gtcgagttca cggcaccgcg acgcccgccg gcagcttgta tttcgaggtc
    18001 ggggcaatca aatctcgagc aatcgcgacc tcgatcaagt tgatggcagg gctagacgcg
    18061 acctcacgca ggcgggcgtc gctctcggct ttgccagcga ggcgcccacg catcgccgaa
    18121 tagaatacgg cttcggtgcg ctcggtcgag ctacgcgcgg cggccaagag cgcttcgtcc
    18181 gagagcttgc cgcgagccca agagcggagc tttgcgggcc agcccgaggt gtcatcgatg
    18241 ctggagtacg cttcttctac ggtgccgtcg ctcatcacgt tcagcttgcg ttccagcaac
    18301 tgcaaccaaa gcgccacgta caccaagtct tccgcaggta ggccggcatc gatcgcgcgt
    18361 tgtgcggcca ttcgcgcggc ctgtagatct ccttgcgtga gcgcgcgccg agccagatcg
    18421 aggatcgtgg ccgagagctg gcgcacgtct ccgctggcgg cctcgaaagc ccgatcggtg
    18481 gctcgcctaa tcgcgttgcg atccccgtac tgttcgagga tgcgagcgag caaacgctcc
    18541 aagcgcgcct gtgcgggccc cgggcgtccc agacgtgtcg cctccacggt gcgggcgagc
    18601 gtctcgtcca gagccttcgc cgccgctgct tccggtccgc gctcgcgcaa gatctcgaac
    18661 ttcgagaaca gcgcctccac ctcggccaac gcgtcgttgt tgcggcgcgc gaggccaatc
    18721 acgcggtcga gtgcatccag cgccgcctgc ggctgctgtt tctgccgcgc gatggcggcg
    18781 agcaggttga aagcgtcgat gctcggttcg agggcgatcg agcgctctac gagcggctgg
    18841 gctcggtcga gctcggcttg gctggcctcc atggccgcca tcacgtagtg gatgcgggcg
    18901 gcgctcggct cgatcttccc cgcgaacgcg cggctctcgg cgagctcgat cagcttttgc
    18961 gctcgtaagt acgtgcgtcg cgctgcaccg aattggccga tgctgttctc cgcaatcgcc
    19021 gcattgagca ccatcgacag ggcgagggac agttcttcgg gggcggcgtt cttgctgagt
    19081 gagctcgaga gcaaaaccgc ggctacttcg gccatgccgt ggccgatcag ttcggccgcg
    19141 agcggcatcg agccatgcaa agtcgcgggt tcggcgcgat agagctcgag cgcacagccg
    19201 aaggcggccg cgcccattac ctcggtgtcg atctcggctt ctggatcttg cgtgagcggg
    19261 cccgtgctct ccgcggccga gcggaacagc cggaacaggg ccgcccaggc gtcgggatcg
    19321 cgctcttcgg cggcgcgctc gaggtgatcc ctcagccccg gagggatgag ccgacccaaa
    19381 tccgcgttgt cgatcgcgtg cagagctccg cgcgcgtccc catgccgcaa atgcaacgcc
    19441 gtgagcacga tcgggccgcc gcgcattgca cggtaggtct ccagggcctc gtcccgatcg
    19501 ccactcaggc gcagtggctg ctcgctgctc gtcgactcga gcgcccgacg catccagctc
    19561 acgagttgct cgcgggcgct gttgaaggct tcggtgctag actcgagcat cgcgcgttgc
    19621 gaggacactc gcgcggcgcg cccgagcgct tgcagtgggc ggctgccttg cgtggcgcgg
    19681 gagaaatcga ggattgcttt gaggtggttt tctacttcgt cgtgagcggg gccgctcggc
    19741 agacccgtcg ccaacatcga gtacaacgcg atcgagaagc cttcttgtcc gagccgagcc
    19801 acctcggcgg caccggcgct caaggctgcg tctgcaccct cggtgaattc gcgccggtgt
    19861 tcacccgcgc gaactaggta gaaagcacct agcaaggccg agagcccggg gcgattgccg
    19921 ttatcgcgtt cgaagcgtgc ggcggcgcgc ttcaattgct gcctcaccac ggcaaccagt
    19981 agccgcgtgc gatcctgact cggttggggt tcgagcaaga tctggtacgc cttctccgcg
    20041 aactcttcct cggagagggg aggcgggttc accgcgcctg ctgaggcatt ggcgctcgag
    20101 gccgttggcc gtgccgccgt cgtgctcgag caacctgcgc tcgaacatac agaaacgacc
    20161 aggcaagcga ggccggcgag ctcgcagaac cggaaaccag acgacatcgt tgggcagtct
    20221 acactccgaa gccgcacggc gccgaagtgt ttggtgaaca ctggctcacg ctgtttccgg
    20281 gcctgactca cggcgcctga ctcacgggcc ctgttctccg ggtgccgttc tcaggcgatg
    20341 tcgagtcagg cttccggttg gcctggaggt gtaggagtgc gcggcgggtg cggacttgcc
    20401 ccacgtgcac gatctagccc acgtgcgggc cgcacgatat gctcatgcgg ccgcgcgctc
    20461 agaaggcgta gtcgaggtgc gcgcgaatcc ccaggatctc ggtgtcgtgc agtcgaccgg
    20521 cgatcgggta gacgaaccca agcgacgggc gaacgtgccc aaagcgagcg cccacgcggg
    20581 gctcggcgac caagtgaaag ggcgacagcg attcggccga cgatacgtac ttgatgcctt
    20641 cgaccgcgtt gtacacgagc caagcatcga gcccgaccca gaaggcaggc ttgtccgtca
    20701 ggtcgtaggt caccccggcg accgtcacgt tccgcacggc gagcgcgcgt tggtggagct
    20761 cgcctttgcc caaaaagatc ttgtcgttgg cgagattgct tcccaaattg aagcccacta
    20821 ccagcttcgt atcggcgtga atttcgagcg cattgcgttc gtgtcgtacg ccgatgccca
    20881 ccacgaccgg caagcgttta gggccgtaaa actcgggatt tcggtagccg ctcgcgccgt
    20941 ccgcgacgag ctggatctgc gccgagttcg aggctcccag atctgcggtg gccggatcgg
    21001 gatcgccttg ggcgaccggt atgcccacac cgaacaggat tggcagctgg gtcagctcgc
    21061 tgagcggatg ccggtactcc gcataaagca gcgggctgcc cagcacggtg cccttgtcac
    21121 tcccgccatt ggtctggccg gtgggcgtcg agcgatcgat gctggccgtg gtgatcggga
    21181 gcttgaggcc caagctgaac ttggcgctga agtcgaactg gccgccgagc acgaagccca
    21241 ccgaggtggc ttctcgcgtg cggtcgttga tttcgtcggc cggccccggc ttcggtgctt
    21301 gaccgaagcc gatgatcgcg tccccgaaga tcaccgcctt gtcgcgaggc ttggcgtgaa
    21361 cgatctcctc gatttcagcc ttctcttcgg ctttctccgc gagcggcccg gtcttacctt
    21421 gcggacgata aggacccttg tcactctgct ccttggaggc tggatcgggc gccgtcgtct
    21481 cgatgctggg gccgctcggt ttttcgttct tcttgccttt tttggtcgct tttccggccg
    21541 ccagggcatt cgcgctcagc aagctcgaga ccaataccaa cgccacgcac cgtcgcatcc
    21601 gtcgcctccg aaacaagagt ccgagaaagt ccgaaaaagg aaagttcgag aaactgcggc
    21661 ctgcaaagcc ggtccgttcg accgagtatt cgccaaacca aagagtattc gccaaaccaa
    21721 accagagcag ctccgcccac gtttgcatcg cgggtgggcc acagcggggc gagacgttac
    21781 cacgctcgct tctgtccgcg gtaatctacg aagaccccga tggtcgagcc gtccgaccgc
    21841 ccgaagcccc aacgcctcgc tgacttcgaa gtcattcgcc gactcggtgc gggcggtatg
    21901 gcagaagttt tcctcgccaa gcgacgtggc gcagaagaca cgtacaagct tctcgtcttg
    21961 aaacgcgtgc tgccggcgta cggcagttcg cgacgctttc gcaccatgtt cgcggaggaa
    22021 gcgcagctcg ctacgcgctt gaatcatccg aacatcgtgc aggtctatga tttccaagat
    22081 tatggggacg agggccagct gctcagcatg gagtacgtgg aggggcccga tctcaggcgg
    22141 ctcatgaagg ccgcagaggc aaaacaacag cggattccgc cgtacgtggc ggcttacatt
    22201 gttgccgaag tcgccaaggg cttgcactac gcgcacgaac gcaaggacga agcgggggcg
    22261 ccgctcgaca tcgtgcatcg ggatgtctcg ccgcaaaaca tcctgctgag ttacgacggg
    22321 gccgtcaaaa tcgctgattt cggcatcgcc tcggccaatc tgtttcgtga agaaccgggg
    22381 gtgctgaagg ggaagaccgc gtacatgtct ccggagcagg cgcggggcga gcgtgtgtcg
    22441 cggcggaccg atatctattc gctgggagtc gtattccacg agctgctgac ggggcgcccg
    22501 ttgcacggcg ccgcggaggg gcaggagttg ctcgaggccg tgcgcagcgg gcacgtcgaa
    22561 ccgccgagca cctgggtgcg tgagctgccg ccggagctcg aagaagtagt gatgcgcgcc
    22621 ttgatgcgcg accccgcgca gcgttttcag acggcacgcg agctctccgc ctcgatcagc
    22681 cgcacgttgc ttcaacacca gcagctgatt gattcacatg tgctcgagac cgtgatcgcg
    22741 cagctcatca cgcgcgagca cacctcaccg ggcgccgtgg gttcggagat cgcgcaggat
    22801 agccaatatg ggccggattc cgtggggcag ccggcgttgt ccagcgtgcc ggagctatcc
    22861 gtcgccattc cgcagcgcga tgacgagcag acggggccgg ggcgtcccgc gcaatcgctt
    22921 cgcgagcgcg cggggcgtga agtgcgtcac gtggcggtgc tcaagttgcg cctccatggc
    22981 gtcgaccagc tggcgcggag cgtgggtccc gcggccgcgg cgcgcgccat cgagcagctc
    23041 cgcggcacgc tcggggaagc cgcctttcgg cgcggcgcga acttcgcctg ggaagctccg
    23101 gaggcggtgg aacctgggtt cgaggtcgtg ctcgccgcgc gcgccgtcgt cgggttgttg
    23161 gcgaaccctt cgcgtgcgcc ggctgacgcc gtgtggctcg cgattgacgt tcacgaggtg
    23221 ctgcgcggtg cgtccgaaga cctatccgtg aagctcgacg tcagtgtcgg tatcgtgcgc
    23281 ggcatcgcct cgggtcgacg tgacaaggcc gggcacctgg tgaaccacga gctgcacgag
    23341 ccctcgggtt atctcgcgga cctattggag cagaacgcgc cacccggagt gtcttgggtt
    23401 gcgggcggct tgtatcgttt ggtacgtcga gacttcgtat ggggggacgc tccgaccatt
    23461 tgtttgccgg aggcggcggc gcgcaatctc cccgaaaata tgcgcattta cgcgctgctc
    23521 cgcccccaca cccgggaaga gaagctctcc gagatggcgg ccttggcgag cgacatcatc
    23581 ggccgggacg ctgaactcgc cgacttgcac gcggcctatt acggcgtcgt gggcaacgag
    23641 aagatccacg gaagcgtggg cgcgcgcgtg ctctacgggg aaatgggcat cggcaagagc
    23701 gcgctgcttt cggcatttct gaacgagctc ccgccggacg cgcaggtgct gcgcgtcgaa
    23761 tgttcaccgg ctctgagtga agtgccgttc gcgacgatcg cagagtgggt gcgcgagctg
    23821 acggagacgc gcatcgacca gaacctcgat caagcgcgcg gcctggtgga agcggcgctc
    23881 ggcgagtacg ccgcgcaaga agaggggcca gaggtgatcg agcgcatggc ggagctcgcg
    23941 accggtcgtg tgcgcgcatt cgaggacgaa gcggacgtcg tccgcagcca caagttgatg
    24001 ggccgggggc tctcgcagtt cttcgcgcgc gccaccctgg acgcccccct cgtggtcgta
    24061 atggatggtt tgcagtgggc agaccggcag agcttggccc tcgtctcgga attcgtgcgc
    24121 cagagcgaag ccttgcccgt gctcgtgctg ctcgcgacgc gccccgacga tcgcgtgctc
    24181 tcggagctcg agggcatggt tcgggtcgag ctcaaaggcc tcggacccga gcaacagatc
    24241 cgcttgttgc aggcgcgcct tggcgtgacc gacggcgtgt tgaccgtttg cgccgagctc
    24301 ctgccgcggg ccgcgggtaa cccattcttc ttgttggaga tggtggacgc gctgctcgag
    24361 cgcggccaaa tcgaaattcg cgaggtggag cccgggcgcc aagagttggt tcaggtcgag
    24421 cgcagcgggg actttggttt gatgctgcct tcgacgctcg aacagctgat cgccgatcgc
    24481 ttgaacgagc tgcccgacga agagcacatg atcatcgact ggctggcggt ggccgggggc
    24541 ccgctctcgc tcaaggacct cgatgcggcg agcgggctcg agaacgcgga cgccgcgatg
    24601 cgtctgtgtg cgcgcgggct ttgcgatctc aagaccgagc tcatcgacgt gcgacatcca
    24661 ttgacgcgtg acgtggctta ccgggccatc gagcgtcgtc gtcgcgtgcg catgcatcgg
    24721 gcgctcggtg agcacctggc caacacgccg ttgtctcgag ggctcaccgc ggccgtggtc
    24781 gctcgtcact tggcgcgcgg gaatgaccgc gccaaggccg ctcgttttta tctagagtcc
    24841 gcccaagccg cccgggccag ctatcagacc cggctcgcgc gacgctactt ccggcgtgcc
    24901 atctcgctgc tgccggaaga tgacctgctg ttactcgagg cgcacgaggc catggaggcc
    24961 atctgtcgca atcaagggcg gttcagtgaa cgtcgccagc acttgatgcg gctacgtaag
    25021 ttgtcacgcg cgagccaacg cgcgcattgg gtggcaactg ccctgatctc gaccgcccaa
    25081 ttcgagctcg acggcgcgcg cttggccaag gcgctagcgt cggcgcagcg cgcggaggtc
    25141 gtcgccgaac gtgcgggcag cccgctgctc ctggtgcaag cgcgcatgct aatcgccgag
    25201 gtgttgcacg acatcggcga tatgcagggg gcgctcgctt cagccgatcg agccgggcaa
    25261 gccgcggagc aggattcggt gcccgcgcgt ttgcgcgcgg aggtgctacg tctgcgcggc
    25321 acgctgctac gccgcgtggg gcgggtgcac gaagccatcg acgcacaggc ggaggcggtg
    25381 gcggtgtttc gacgagtcgg ggccaggcgt ttggaagccc gcgccaagaa ctcgctcgct
    25441 tacggtctgc tcgtgctcgg gcgcttcgag gacggcatca tcttggcctt ggatgccatc
    25501 cgtatcgatc tctcgattgg gggccggttt caaatcgcca agaccctgaa cacgattggg
    25561 gagtgttacg cgagcctggg cgacttcgat cggggcatcg cctacatgcg acgcgcgcgc
    25621 gacgcccacg agcgctacgg cgatcaagac tcgcgcgcag acacgctgct ctcgttatcg
    25681 gaggccttga tcgaaattgg agacgcctct gcggcggagc cgctggtggg cgacgcgggt
    25741 gcgctcacgg ccgtgaccgg cagcgcttac gactcggtgc acgagaaaat cctgagagcc
    25801 gtgctcgcgc gtttggcgca cgaccccgcg cgcgccgtga tgcacgcctt cgacgctcgt
    25861 caggccgcgg aagcgcaggc ctatgtcgcc ttccattttt acgccatggc cgtcgaggcg
    25921 tgtgcgcgcg tcgagatggg ggagcaacat accggcattc tgctcgcgac gacggcgatg
    25981 ggagcgatcg agacgttgca gggctccgag tatggcctgc atacccgcgc gttgtgtttt
    26041 cgggccctgc gcagcgcggg ctcgccccaa gcggcggagg cggagcgccg cgcgaccgct
    26101 tacgtgcaac gtttgctgga gcagatccgg gatccagcgt ttcgccgcac ctttcgccaa
    26161 cgcccgctgg tgcgggagat cctcggggac gtgaaagaga gcacggggaa ctgagcggta
    26221 agtactcaga gtccccgcgc ttgcgcgaat cgagagagcg gagattcgat tcagtcgttg
    26281 cagccgtagt ccacggtctc gttcgaaccc gcgggccaac cattggagcg cccaaagccg
    26341 gagctcgtgg ttcgcacttc gccgtggggc accacgatgc gcgtacccga gtagttcgtg
    26401 cccgagaaca cgcagacccg ggctttgttg ttcacccggt agtcgatgta cccgaagtac
    26461 ttggcgcggt tcgcccaatc gaggtccgcg aagttgccgt ttacgccctt gagcagcttt
    26521 gccgcgccgc tgccgttcgt gccttcggcc aagcacaacc cgccagatcc ggcggcggcc
    26581 ttgcatgccg tcgggaagca caccgcgact tttccgctca ccgatgaggt gcggcacggc
    26641 gcgctcgcgg gacaagtcat gttcgcgcaa ttgcccgtat cgcagcgcgt gccgccccag
    26701 cccgcgctgc acgcacattg gtagccgttg accttgtcgg tgcaggtgcc atgcacgcag
    26761 ggcttcgact tgcattcgtc gatgttgttc tcgcaattca cgccgtcgta gcccgcaggg
    26821 caggtgcact tgtacgcacc gttcgtcaga tcggtgcagg tgccgcggac gcagggcttc
    26881 gagacacacc ccgagatctg cgtctcgcag ttctttccgc cgtaaccggt cgggcaggtg
    26941 cacttataat cgttcaccag atcgctgcag gtgccgcgga cgcacgggga cgccgcacaa
    27001 tcgttcacgt tggtctcgca gttggttccg gtgtaacccg cctcgcactt gcacgtgtaa
    27061 gacttgagac cgtcgacgca ggtgccgtgc aggcaggggt tcgcggcaca atcatcgatg
    27121 ttctcttcgc aggtcttgcc tttgaatccg gtgctcgagc aatcacaaga gaacgagccg
    27181 atcccgtctt tgcactgacc cccgttcttg catggagcgg ccgcgcaatc gtcgatgttg
    27241 gtgcaagtct gcccatctcc ggtgaagccc gccgcgcaga cgcattgatt gtctttcgcc
    27301 ggatcacaga acgcgttttc gtcgcagttc ttggcctcac aacccgagtc gaacgtgcag
    27361 gttttaccgt cgcccacgta cggggaagcg caagtgcaag tcacctcgcc gtccaccgcg
    27421 gcgcaggttg cgtgcggatc gcaaccggcg ctctcacagc tcgagacgca gcgacgatcg
    27481 ttttcggggt cgagctcgta acctgcctgg caatcgcagt cccgtccacc cgggaagttg
    27541 atgcaaacgg cggtggcgtc gcagccgccg ttttccacgc tgcattcgtc gatgtcgaag
    27601 caagtgccgt ccttgctcgc ggcaaacccc tccaggcagg cgcagacggc gttcgcgcca
    27661 tcccactgac attcggcgta ctgcccacac tccggttcgc agacgggctc aggcgtcgac
    27721 gtggatccac cggtgctcgt ggatccaccc gattgaccat ccccaccctc ggcgacgccc
    27781 tccaaaccac cgctcgctgt ttgcccgccc gagctttggg gcttgccacc gttggtgccg
    27841 ccgacgttgc ttcccgaaga ccctccctgg ctgctgtctc caccttttgc cgaatcatcc
    27901 cgagagcccg gttcattccc gagcagaagg ttgcaagcag acgagaggcc catcgcgaag
    27961 ccagccgcca tcgccaactg ggtagttcgg aagtgccacg agcgagccga aacgcgaccg
    28021 tatgaacgag tcactttcat cgagttaacg cgctcttagc actgcggcgc gggattcact
    28081 cgggcaggcg gccgatggac ttcaaaactg agcaaaaatg accagaattt cgattcaatt
    28141 cggcggggct ctccgggtgg gcttgggcct cgtcattcgc gcgtgccctg cagtttgcgg
    28201 aacagtcatg ctcgcgggct acaatcctgg ccgcgatgca gccgcttcgt tccgtcggcc
    28261 tgggcctcgg tggtgtgggt ctagggttgg tcggtttggg gctggtcgcg atcacggtgc
    28321 gactcgggtg ggcgaatttc gttgcaggtc acgtgccggc cccgcccgtc ggggcgtcgg
    28381 cgctttcctc gggcctcccg aatgtgctcg cattgcgctc ggattctgga gccgcgaagg
    28441 ggacagacga tcgttcgaat catccgcaga gcctgctccc acgaa
//
LOCUS       DASTKU010000838         8670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_833482, whole genome shotgun sequence.
ACCESSION   DASTKU010000838 DASTKU010000000
VERSION     DASTKU010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833482"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..911)
                     /gene="tssJ"
                     /locus_tag="VFQ61_29375"
     CDS             complement(273..911)
                     /gene="tssJ"
                     /locus_tag="VFQ61_29375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HET9958654.1"
                     /translation="MNPEAPVAHFQVCSRPQVRLLASHSRTEVGRSGRLRGPFTLGLV
                     ALGFAALACGSSAGPPANSTPPPEPCKPVQISLALASSNQVNPDPSGQGRPVQVRIYQ
                     LKSDARLRTASFEDVWQSDKTALGEDYIQVDQQTLYPGENKLYPVKPATGMQYLAVVA
                     LFREPQGKDWFVSYQIDPPRTKPPCPDKDARIPVWLDRMQIQDGAGRETASP"
     gene            1625..2116
                     /locus_tag="VFQ61_29380"
     CDS             1625..2116
                     /locus_tag="VFQ61_29380"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HET9958655.1"
                     /translation="MAVNIHLKLAGIDGESATKGYEKQLEVLSWQVGVTQTASAHFAE
                     GSGTGKCDISDLTITKYVDKSSPILFGHSASGKHIASGQLTLKKAGGDESVIYMTIDM
                     EDVIVSAYNVGGTPSDDRIVETVTLNFGKVHMAYKPQDKKGAGGADIKAGWNIPQNAT
                     WSK"
     gene            complement(2396..5194)
                     /locus_tag="VFQ61_29385"
     CDS             complement(2396..5194)
                     /locus_tag="VFQ61_29385"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET9958656.1"
                     /translation="MRSSAGPDAATEGQARARFVADLSACLEDWRRALESGLVPSDPR
                     LRALRQLSQELERRAASVAFGGVGHHLRSLLALVDAGVLDPRALAQTLEVLRDMREQA
                     LMALSPGDRQLATAPKQQASSAGGAALPPPLITIMKPAGGPSGPPPIGNGPPPLMSGG
                     LSGSNPGPPPLAPIPALPDFANQRLSPLPGDGGGAGLGLPGNSPQPPVLSPIGAQPDL
                     FHGGSGAPLPLAPISGGLLGMGSSGANANFPSASGPPSNGPGPKPIVGAGLPPLGSAP
                     VGGLPVTGGPPAGGPRILGSAGGAAGGGGFAPVPNAPAAAPGAQPNLLVRSMLGLRGF
                     GRGKPGKPEGAPADAPPPPMNANASSPGILGLGARNKSRSSQSTPAVVAPPPLVADDP
                     SGLPALHDLRPGSSSSPSSPPGGASPGAGSSAPPGLPGLPGLPGSRNRTTGRNLATGK
                     NQTGKNEVLEKILLARDTGPRDARGPKRKRFQRRPDGADADRRVGLIAGIGLAVAVGS
                     IFASIVLVQTCDKPPPPPPPRADAGVSADAGQVELPTGGLLSEAERRLYFMNKQIREH
                     GEISPELRSLLDEEVALRREEIKLCGDRPCTAKDELAPNKARAVQPRKARTVVAGIAP
                     KWLAGLALPSIPIEDDPRVESWFQKYTQSRELKEALQQSVFRCSQYREMVRTKFTRFE
                     LPTDLLAVAFWESECRWDAKSKAEAVGMWQFTKAAARAYHLLVIDNVVDERLNPAKAT
                     DAAIRYFSDMKKKVNSWDLVFAAYNMGPFGLMARIEQAGGGVSFFDLADAEMIPKETM
                     NYAPAIQAIAVILNNLEALKFNDQKKMPVFTEGLEVPPNTRLTMVARAASTTVRALKE
                     LNRDLLADTTPSVEGFAVQVRPDVVWQAREELKKFITMRDDGDLCMPPETDWNVAQLT
                     PAKRAECQKRREKATPER"
     gene            5751..6830
                     /gene="tssA"
                     /locus_tag="VFQ61_29390"
     CDS             5751..6830
                     /gene="tssA"
                     /locus_tag="VFQ61_29390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HET9958657.1"
                     /translation="MAIDLSKLLEPVSEAEPTGPNLEYDPAFSALERATEGKPERQAG
                     GTILPAEPPEWRAVIEPAQALLLRSKDLRVATHLALALLNTEGYVGLSQGLALVRSLL
                     ETYWPTLHPTLDPEEDNDPTMRITALAALATPAVLQIMRGKPVVSGRTSGSFSARDVL
                     NLRDTTATPPDPAAIEAAFRETDLESLARTTEAAQAALEHVRGIDAVFEAQTGSRGPD
                     FGALAQLLHQVRTAVGQRLEQRRQSEQPSSSETTDAGGSDAGAGGSAGAAPANRPMGG
                     GRSLSGEIQSRDDVVRALDKICDYYDRYEPTSPLPLLVQRCRRMVSMRFMDIMKELVP
                     EGIATVEVITGKTGASAEGSEGESE"
     gene            6961..7470
                     /gene="tssB"
                     /locus_tag="VFQ61_29395"
     CDS             6961..7470
                     /gene="tssB"
                     /locus_tag="VFQ61_29395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HET9958658.1"
                     /translation="MSSQKFIGRNRAPRVQIEYDVELYGAEKKIQLPFVMGVMSDLSG
                     NAGKPLPPVADRKFLEFSVDNFDDRLKAIEPRVQMDVKNTLTGEGNMKVDLTLESMND
                     FTPGAIARKVPALQKLLEARQQLANLITYMDGKTGAEELIAKILNDKTLLETLAASPK
                     PESEDKKSE"
     gene            7474..>8670
                     /gene="tssC"
                     /locus_tag="VFQ61_29400"
     CDS             7474..>8670
                     /gene="tssC"
                     /locus_tag="VFQ61_29400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HET9958659.1"
                     /translation="MSEATEKAVAQPGVLTQEGNDFTALLNKEFKPQSDRAREEVESA
                     VMTLARQALSSTATFSSDAVMSIQAIIAELDRKLTEQVNEILHHKDFQALEGSWRGLH
                     HLVNNSETDSMLKIRVMNVSKKDVHKTLKRFKGAAWDQSPLFKKLYEEEYGQLGGEPY
                     ACLVSDYYFDHSPQDVETLGELAKIGAACHAPVITAANHTLFQMESWQELGNPRDLAK
                     IFSTPEYAAWRSLRESEDARYIGMCLPRFLARRPYGAKSDPVEEFDFEEDTNGPDATK
                     YTWANSAYAMAVNINRSYKLYGWCSRIRGIESGGAVEGLPTHTFPTDDGGVDMTCPTE
                     IGISDRREAELAKSGFMPLVHKKNTDFAAFIGAQSLQKPAQYDDPDASANAQLSARLP
                     YLFATSR"
BASE COUNT         1703 a         2727 c         2735 g         1505 t
ORIGIN      
        1 tgctggctga ccagcacgcc ttccgtccaa attggcttat gcggcatgtc gttcacccct
       61 gaaattactg ccgaacaccg tcagaaatcc gcctgcaaag ccgggcgtcg atgctgtgct
      121 cgagcccacg cacgccccgg cggagccagt gagctcggac gagccagcaa tcgaatgccc
      181 caggcgttcc gccccgtctt gtttcagcgt gtgattttct tccatcgtgt ccgtgcgcac
      241 gggtgctgag cgtttttcat acgacgccgg gtttatggcg acgccgtttc gcgccctgcc
      301 ccgtcctgaa tctgcatgcg atcgagccaa accgggattc gcgcgtcctt gtccggacaa
      361 ggaggcttcg tgcgcggagg atcgatctga tagctcacga accaatcctt gccctgcggc
      421 tcccgaaaca acgccaccac ggccaagtat tgcatccctg tcgccggttt gactggataa
      481 agtttgtttt cgccgggata gagggtttgt tgatcgactt ggatgtagtc ctcgccgagc
      541 gccgtcttgt ctgattgcca cacgtcctcg aagctcgcgg ttcgaagccg tgcatcggac
      601 ttgagttgat agatccgcac ttgaacgggc cggccctgcc ccgacgggtc cggattgacc
      661 tggttcgagg acgcaagcgc caaggagatc tgcactggct tgcacggctc gggtggaggc
      721 gttgaattgg ccggcggacc cgcagagcta ccgcaggcca gcgcggcgaa gccgagcgca
      781 accaacccta gagtgaacgg cccgcgcaac ctgcccgaac ggcccacttc ggtacgcgag
      841 tgcgaagcga gaaggcggac ttgcgggcgg gagcaaacct gaaaatgagc gacgggagcc
      901 tccggattca tgggaggaaa tgctttatac acgagccagc ccgacccggc tcacttctgt
      961 tctcccgggc cacgatccca cggtagacgg gcggcttttg cgtccgttcg ggacgcgccg
     1021 aaccgaccaa tcaggcgaat cccgagactt tacggcccag ctacgccgat gccggctgtg
     1081 tttaggggga acctgtctat cgcggatccc tgtccacccc aacccgtcca atcaccacct
     1141 tggccactca gtgcgcctat ttttagctat gccgatctgg gcgggcccct cgagcccgcg
     1201 ccgatcgcgc tcccagatcg atccaaactg gcggccctcg cgtatcttgc gcatgaagtg
     1261 caggtcacga taggcctgtt tgtgagtatg gattatgatg cgctcccgaa cggtgcgccg
     1321 aacgtagtgt tctgattgcg atcacttgac ccaaccgaaa cttgcgtata ctcggttagc
     1381 atcgcatcgc gggtgatgca tggtggcaat cgcgtgggtc gttggatagc gcttactgac
     1441 gtcgcgtctc gggaaatcaa aaactcgaaa cgtgaggtag gcgggaagaa aagtaggcga
     1501 tgagcggtaa caatgagagc cgtttcatgg cggggttggt ttcagcgact cacggacgcc
     1561 tcgaactgaa agagcgcata gcaattgcgc agtctcactg cgcagccgaa ggagacaaag
     1621 tcagatggcc gttaacattc atttgaaatt ggctgggatc gatggcgaga gtgcgacgaa
     1681 ggggtacgag aagcagctag aggtgctcag ctggcaggtg ggcgtcacgc agacggccag
     1741 cgcgcacttc gccgaaggca gcggcacggg gaaatgcgat atcagcgacc tgacgattac
     1801 gaagtacgtc gacaagtcgt cgccgatcct gttcgggcac tctgccagtg gtaagcacat
     1861 cgcctcgggg cagctgaccc tgaagaaggc cggtggtgat gagtcggtga tctacatgac
     1921 catcgatatg gaagacgtta tcgtctctgc gtacaacgtc ggcggcacgc cgagcgatga
     1981 ccgcatcgtc gagaccgtca ccttgaactt cggcaaggtc cacatggcct acaagccgca
     2041 agacaagaag ggcgctggcg gcgctgacat caaagcgggc tggaacatcc cccagaacgc
     2101 tacttggtcg aagtaatcgc ctgaggtttg cagatggccc gctccaatgc tttggcgcgg
     2161 gcctttcgcg cctagagctc gagaaactcc agctcttcgt tatataaagg gctccgatga
     2221 actcgggccc tcggtgccta gggctcgagg aaactcgggc ctttcgttta tctgcacgtt
     2281 ggttcgattg cgtgcgattg cagactcgca atcgccaggt ccggggcggc acaccggcgc
     2341 aagcaaggcc gccagcgaga gcacgaagcc atgagcggat ggcggggaag caccgttagc
     2401 gttcgggagt ggccttttcc cggcgcttct ggcactccgc gcgtttcgcg ggcgtgagct
     2461 gcgcgacgtt ccagtctgtc tccgggggca tacacagatc gccgtcgtcg cgcatggtga
     2521 tgaacttctt gagttcttcg cgggcttgcc acaccacgtc ggggcgtacc tggaccgcaa
     2581 atccttcgac gctcggggtc gtgtcggcga gcagatcgcg atttagttct ttcagcgcgc
     2641 gcaccgtggt ggacgcggcg cgggccacca tcgtcaagcg cgtgttcgga ggcacctcga
     2701 ggccctccgt aaacaccggc atcttcttct gatcgttgaa cttcagcgct tcgaggttgt
     2761 tcagaatcac ggcgatggct tgaatcgcag gcgcgtagtt catggtctct ttggggatca
     2821 tctctgcgtc cgccaaatcg aagaagctca caccaccgcc ggcctgttcg atgcgcgcca
     2881 tcaagccgaa tgggcccatg ttgtatgcgg caaacaccaa gtcccaggag ttgaccttct
     2941 tcttcatgtc cgagaagtag cgaattgccg catccgtcgc cttggcggga ttcaagcgtt
     3001 cgtccaccac gttatcgatc acgagcaagt gatacgcgcg cgcggcggcc ttcgtgaact
     3061 gccacatccc aacggcttcg gccttggatt tggcgtccca acggcactcg ctctcccaga
     3121 acgcgaccgc tagtagatcg gtcggcaact cgaagcgcgt gaatttggta cgcaccatct
     3181 cgcgatattg gctgcagcga aataccgatt gttgaagggc ttccttcagc tctcggcttt
     3241 gcgtgtactt ctggaaccaa ctctcgacgc ggggatcgtc ctcgatcgga atgctgggta
     3301 gcgcgagccc cgcgagccac ttcggcgcga tccccgccac cacggtacgc gccttgcgcg
     3361 gctgcaccgc gcgggccttg ttgggcgcaa gctcgtcttt ggccgtacac ggcctgtcgc
     3421 cgcacagctt gatctcttcg cggcgcagag ccacctcttc atcgagcagg gagcgcagct
     3481 cggggctaat ctcgccatgc tcgcggatct gcttgttcat gaagtagagg cgccgctctg
     3541 cttccgacag caatccgcct gtagggagtt cgacctgccc ggcgtccgca ctaacacccg
     3601 catcggcccg gggcggcggc ggaggcggag gcttgtcaca ggtctgcacc aacacgatgc
     3661 tcgcgaagat gctgccgacc gcgaccgcca gcccgatacc cgcgataagg ccaacgcggc
     3721 ggtcggcgtc tgcaccgtcc gggcgccgtt gaaaacgctt tctctttggc ccgcgcgcgt
     3781 cacgtgggcc cgtgtcccga gctagcagga tcttctcgag cacctcgttc ttaccggtct
     3841 gattcttacc ggtagcgaga ttcctacccg tagttcgatt gcgactgccg ggcaaaccag
     3901 gcaggcccgg caagcccggc ggagcgctcg aaccagctcc gggagaagcg ccgccaggcg
     3961 gcgagctcgg cgacgaagag ctgcccggtc gtagatcgtg cagtgccggc aacccggaag
     4021 ggtcatcggc cacgagcgga gggggcgcaa cgaccgccgg tgtggattgc gagctccgcg
     4081 atttgttgcg cgcaccgagc cccagtattc cgggcgatga cgcattcgca ttcatgggcg
     4141 gaggcggggc gtccgcaggc gcgccctcgg gcttgcctgg ttttccacga ccaaacccgc
     4201 gcaaacccaa catcgatcgc acgagcaaat tgggttgggc accgggcgcc gccgcgggcg
     4261 cgttgggaac gggcgcgaaa cctccgccac cggcagcgcc gccagccgat cccaagatcc
     4321 gcggcccccc cgcgggggga cctcccgtaa ccggtaaccc tccgaccggc gcagatccga
     4381 gcggcggcag cccggctccc acgatcggct tggggccggg gccattcgaa ggcgggccac
     4441 tcgcactcgg gaagttcgcg ttggcccccg acgagcccat gccgaggagc ccacccgaaa
     4501 tcggagcgag aggaaggggc gcgcctgagc cgccatgaaa caagtcgggt tgggcgccta
     4561 tgggcgagag cacgggtggc tgcggcgagt tgccgggcaa gccgagcccc gcaccgccgc
     4621 cgtcacccgg aaggggagag agacgctggt tcgcaaaatc gggcagcgcg gggatcggtg
     4681 cgagcggagg gggccccgga ttcgacccgg agaggccccc gctcatcagc ggaggcggcc
     4741 cattcccgat tggaggcggg cccgatgggc cacccgccgg cttcatgatc gtgatgaggg
     4801 gaggtggcag cgcggcgccc cccgcactcg aggcttgctg cttaggggca gtagcgagct
     4861 gccgatctcc cggcgaaagg gccatcagcg cctgctcgcg catgtcccga agcacctcga
     4921 gcgtctgagc aagtgctcgc ggatcgagca ctccagcgtc gaccaaggcg agcaggcttc
     4981 gcaagtggtg ccctacgccc ccaaaggcca cgctcgcggc gcggcgctcc aactcctggg
     5041 acagctgacg caaggcgcgc aggcgcggat ccgaaggcac gagcccggat tccaacgcac
     5101 gacgccaatc ttccagacac gcgctcagat ccgcgacgaa acgcgctcgt gcttgccctt
     5161 ctgttgcggc gtcgggaccc gcagacgatc tcacgggttt ccattagcac aagcccgcat
     5221 ccaaaccccc aaacgaagcg agtcatgcat cgtcattggt caggcccgcc cgagggtagc
     5281 gccgcccaag cacgctgaat cattgccggc tgagccgccc gcgtacgagc ttgcccaaac
     5341 taagactgcg cctcgaacaa gccaacacgt tgacatcgca ccgcgtctgc ggtgttaata
     5401 gctgcgttcg gttggttcgg ggctctcgtt ttgggcggtg agcttttttg ggagcaagct
     5461 ttgctctggc agtgtgtgga tggtcgatcc acagctgttt ggatgcctgg cctttgattg
     5521 ggatgctgtg tgcgcgcact gcacgggtca ttcgcctatc tcaaggcaac ggccagtttc
     5581 gagccgggct tctaccgagc cggtcccatt gaaagcgatc ccgatcgagg cgaggcgccc
     5641 tatcgaacta gtcggggccc atcgagtcga gtagggtgct cgaacccctc cgcaaagggc
     5701 tcgaaccgag gccccggggg cacgttagaa acaattacca cgtcgttcac atggcgatcg
     5761 atctgagcaa gttgctggag ccggtctcgg aggcggagcc tacggggccc aatctcgaat
     5821 acgatcccgc gttttctgcg ctggagcgcg cgaccgaggg caaaccggag cgccaagcgg
     5881 gcggcacgat tttgccggca gaacccccgg aatggcgcgc cgtgatcgag cccgcgcaag
     5941 cgctgctgtt gcgtagcaag gatttgcgag tggcaaccca cctcgcgctc gcgctgctca
     6001 acacggaagg ctacgtcggg ttaagccagg gcttggcgct cgtgcgaagc ttgctcgaga
     6061 cctactggcc caccctgcac ccaacgctgg atcccgaaga agacaacgat ccaaccatgc
     6121 gcatcaccgc gctcgcagcg ctcgcgaccc cggcggtgct gcagatcatg cgtggcaaac
     6181 cggtcgtgag cggcaggacc tcgggctcct tcagcgcccg tgacgtcctg aatttacggg
     6241 atactacggc gacgcccccg gacccggccg cgatcgaggc tgcgtttcgc gagaccgatc
     6301 tcgagagcct cgctcgcacg accgaagccg ctcaagcggc gctcgagcac gtccgcggaa
     6361 tcgacgcagt attcgaagcc caaacgggga gtcgcgggcc tgacttcggc gctctcgcgc
     6421 aactgttaca ccaggtgcga accgccgtcg gtcaacgcct ggaacaacgc cggcaaagcg
     6481 agcaaccgag ctcatccgag acgacggatg ccgggggttc ggacgcgggc gcgggcggct
     6541 ccgccggtgc agcgccggcc aatcgcccaa tgggcggcgg cagatcgctg tcgggggaga
     6601 tccagtcgcg ggacgacgta gtccgagccc tcgataaaat ttgcgactac tacgatcgct
     6661 acgaacccac gagccccctt cccttgctcg tccagcgctg caggcgcatg gtcagcatgc
     6721 gtttcatgga catcatgaaa gagctcgtac cggagggcat cgccacggtc gaggtcatca
     6781 caggcaaaac gggtgcatca gccgagggct cggaaggcga atcggaatga gcgggctcgg
     6841 agcaaacaga ctcggagcga gtggagtcaa cgcgagctca gcggggcatt gctgcgggaa
     6901 ttcccgagcg caacgaccaa cgggcgaaac gattacgggc gaacatagga gagtgtgtca
     6961 atgagcagtc agaagttcat cgggcgaaac cgggcgccgc gcgtgcagat cgaatacgac
     7021 gtggagctct acggggccga gaagaagatc cagctaccct tcgtgatggg tgtgatgtcg
     7081 gacctctcgg gcaacgcggg caaaccactg ccgcccgttg ccgaccgcaa gttcctggag
     7141 ttctcggtcg acaacttcga cgatcgcctg aaggcgatcg agccacgcgt gcagatggac
     7201 gtgaagaaca cgctcaccgg tgagggcaac atgaaggtgg acctcacgct ggagagcatg
     7261 aacgacttca cgccgggcgc gatcgcccgc aaagttccgg ccctgcaaaa gctactggaa
     7321 gcgcgtcagc aactcgccaa cttgatcacc tacatggatg gcaagaccgg cgcggaggag
     7381 ctgatcgcca aaattctgaa cgacaagacg ctgctggaga ccttggccgc gtccccgaaa
     7441 cccgaaagcg aagacaagaa aagcgagtga gccatgagtg aggcaaccga gaaagcagtc
     7501 gcccagcctg gagtcctcac ccaggagggg aacgatttca ccgcgcttct gaacaaagaa
     7561 ttcaagccgc aatccgatcg cgcccgcgaa gaggtggagt cggcggtcat gacgctggcc
     7621 cgccaggcgc tctcgagcac ggccacgttc tcgtcggacg ccgtgatgtc cattcaggcc
     7681 atcatcgcgg agctcgatcg caagctcacc gagcaggtca acgaaatcct gcatcacaag
     7741 gatttccagg cgctcgaagg ctcctggcgt ggcttgcacc acctggtgaa caacagcgag
     7801 acggactcga tgttgaagat ccgggtgatg aacgtctcca agaaggacgt ccacaagacg
     7861 ttgaagcgct tcaaaggcgc cgcttgggac cagagcccgt tgttcaagaa gctgtacgag
     7921 gaagagtacg gtcagctggg cggtgagccc tacgcttgcc tcgtctccga ctactacttc
     7981 gaccacagcc cccaagacgt ggagacgctc ggcgagctcg cgaagatcgg agctgcgtgt
     8041 cacgcccccg tgatcacggc cgccaaccac acgctgttcc agatggagag ctggcaagag
     8101 ctcggtaacc cgcgcgattt ggccaagatc ttctccacgc cggaatacgc ggcctggcgc
     8161 tcgctgcgtg aatcggaaga tgcgcgctac atcggcatgt gcttgccgcg ctttttggcg
     8221 cgccgcccgt acggcgccaa gtcggacccg gtagaggagt tcgatttcga ggaagacacg
     8281 aacgggcccg acgcgacgaa gtatacgtgg gccaactcgg cctacgcgat ggccgtcaac
     8341 atcaaccgct cttacaagct ctacggctgg tgttcgcgca ttcgcggtat cgagtctggc
     8401 ggtgcggtgg agggcttgcc cacgcacacg ttcccgacgg atgacggcgg cgtcgatatg
     8461 acgtgcccga ccgaaatcgg gatcagcgat cgccgcgaag ccgagctcgc caagagcggc
     8521 ttcatgcccc tcgtgcacaa gaagaacacc gacttcgcgg ccttcatcgg cgcccagtcg
     8581 ctgcaaaagc cggctcaata cgacgatccg gacgccagcg cgaacgcgca actctcggct
     8641 cgcttgccct acctattcgc gaccagccgc
//
LOCUS       DASTKU010000839         4638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1505828, whole genome shotgun sequence.
ACCESSION   DASTKU010000839 DASTKU010000000
VERSION     DASTKU010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505828"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1025..1870
                     /locus_tag="VFQ61_29405"
     CDS             1025..1870
                     /locus_tag="VFQ61_29405"
                     /inference="COORDINATES: protein motif:HMM:NF012647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HET9958660.1"
                     /translation="MLDERRRVAREGWGAGLLSLQDPEGTWGGGLYNPKWTSTTYSML
                     LLRDLGLLPNITSTRASCERLLNEGLRSDGGVNFGAKASETCITGMILSIVSYFQYED
                     ARAGELVRYLLREQMTDGGWNCRRRLGATHSSVHTTINVLEGLHLQEGGQRSVELRRA
                     AERGREFLLQHRLFRSHRTGRVIRPEFTRLSFPPRWHYDILRALEHFRAVDAPRDTRL
                     AEAVELVRNKMRPDGCWVLENSYRGRSFFELERVGQPSRWNTLRALRVLHWWDVGTRA
                     PSKPG"
     gene            complement(2422..3807)
                     /locus_tag="VFQ61_29410"
     CDS             complement(2422..3807)
                     /locus_tag="VFQ61_29410"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9958661.1"
                     /translation="MMRQLLLLVVTAMLALTVAQEAAAQTPTAPPSRAPIVEAQAPPT
                     PRALSLREAVGIAVRQHPNVRGADARVRGLEAQIDVTRAAYLPTVSGTLTGAVGHTNR
                     PVLPDVRVQSTYGVAEASLTGQALVYDFGRTANAVRAAEQAHAAGTADANVLRYQVAL
                     DAANRYLLVLSDRELVEVARVTLRQRELQANVTRELVARGLRPALDQNRAQLEIDIAG
                     FEIEAAEARVAQDLAALAASLGLDPSTPLALQALDPTNLGVDPDLQRAAAAAQIHRPE
                     IQAARAHLEHDRARIDATRALARPVLSVLANGVINRQDVISGYGLAGFSETVQAGALL
                     SVPVLDFTVSPRVRVTESERDSAEQALHAQLLTVRAEATQAAIAARAAESMHVRAEHI
                     LRSATLNLNLAQERYAQGVSPLIEELIEAQSREQTARVNLVGARLSLQLARIQLLAAT
                     HGARFPEHLGE"
     gene            complement(3804..>4638)
                     /locus_tag="VFQ61_29415"
     CDS             complement(3804..>4638)
                     /locus_tag="VFQ61_29415"
                     /inference="COORDINATES: protein motif:HMM:NF025394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HET9958662.1"
                     /translation="RTRKQTNGWFQANVQLSSDRVAARDQALALFAALGPEIKRWRAQ
                     GELKRFFFMRKPPGLRLRFRFVERSALTRLQMRLHELGGQGAIQGWAPAIYEPEKYKW
                     SPSLVPHVHAFFDADSAAFVRWFPRQRQGQSALTAPTLSLAVLSDLFSRATESRDEAW
                     DVWCNLQRLHGSGMHNAVEPTGSARVREFESFGLRQLAERVPEPDRRLLLSYERTNTR
                     FANALNNALHLGSLTQGPRAILAFLTMFHWNRWGLSLSERAALYEGMIDTLHPARSLK
                     A"
BASE COUNT          813 a         1365 c         1580 g          880 t
ORIGIN      
        1 tctttctcga ggtatacaag agggcattcg acgtctccta ccattggttc agtgatgaag
       61 acggcctccg actcctcagc aagcacccag actcctgaaa tcgatgagct gactctgctc
      121 ctcgaggccg caaggcgcgc caactgggac gctatgcacg gtccgagaca tcttcgttcc
      181 gggcgcttct tcatttccga aaacttgaac gcgcatgctt cgaacagact gtcatcgacg
      241 gcgcaagcac ccgaacagcc gagttccgac tcaacacgca cggtcgccta acgaattagc
      301 gttttcgcgg cggcgcagcg ggcgagcgaa gcgagccgcg aagaccggcg ctaaaacgcg
      361 agttgaacaa gcccgagaga ggcggcctag cgcgcgccaa tgggtattgc acggtgtgat
      421 gctcctcacg tccgaagcac atgcatggtt tgctcgcggc agagctttga gcgtgcgaga
      481 taagccgttg gtgctgcccg ggcgatcagt gatctcgagt gacagctagg cgcaacatgt
      541 tcgtgttgat ggacccgcgc cgacacgctc ggtgtagcgc ggataggtag tctccctcgt
      601 tcacgcagag gcccccgttc tggccacggc cgtcccggga gttcgaggcg gagctcgcgc
      661 cgggtccacg agggcgcgct ccgttgcgcg cactcgagcg cgcgctcagg cgacggacga
      721 cgcggagtgc cgtttgatga cagtgcgggc agcgccgcgg cgaagccttt ctctggcatt
      781 gatatcgttc tagatcgtcg cacatgacgg tcgccggcaa ctttgtgttg aacaccgacg
      841 aaaccgtcgc ttcgctcggg ctttcgcggg ttgacactgc tacgttggac ggacgagcgg
      901 gaagccgtcg tggagaaggt cgaggcgcgt gggcgatccg gacgtaatac gctggctgct
      961 taaaggggac cccgccattc ggtggcaggt atttcgagac ctgctgaagc ggccgtcccg
     1021 agcagtgctc gacgagcgac gaagggtcgc acgtgaaggt tggggcgccg gtctgttgtc
     1081 cctgcaggat cccgagggga cttggggggg tggtctgtac aacccgaagt ggacgtccac
     1141 aacctacagc atgctattgc ttcgtgatct cgggctgctc cccaacatca catcgacgcg
     1201 cgcctcatgc gaacggttgc tcaacgaagg cctccgatcg gacggtggtg tgaacttcgg
     1261 ggcgaaggcg agcgagacat gcatcacagg catgatcttg tcgatcgttt cttacttcca
     1321 gtacgaggac gcgcgggcag gagagctcgt caggtacttg cttcgtgagc agatgactga
     1381 tggggggtgg aactgccggc gtcggctcgg agcgacgcac tcgtcggtgc acacgacgat
     1441 taacgtgtta gagggtctgc acttgcaaga gggcggccag cgatcggtcg agctccgacg
     1501 cgcagcagag cgcggtcggg agtttcttct gcaacatcgg ttattccggt ctcatcgcac
     1561 cggacgcgtc attcgaccgg agtttacgcg cctgtcgttt ccgccacgtt ggcactacga
     1621 catcctccgc gccctcgagc acttccgcgc ggtcgacgcg ccgcgagata cacgcctggc
     1681 cgaggcagtg gagctggtcc gcaacaagat gcgccccgac ggctgttggg ttcttgaaaa
     1741 tagctatcgg ggtcgctcct tcttcgagct cgagcgagtc ggtcagccca gcagatggaa
     1801 tacattacgt gcccttcgag tcctacactg gtgggacgtt ggaacacgag cgccgtccaa
     1861 accgggctga agcagactcg ggtctcagcg ctccatccgc gcagcttagc cctggtacgt
     1921 tgggcaggcg cgtcagggcg gatattgagt tcatttacta gtgctctggt gaggtttgca
     1981 gtttggatgt gtctgtcggg tacttccacg agcacagtga tttactgcaa cacttcgcgt
     2041 ctctgaaatc taatctgacg tgtcggctct ttttcgctat cgccttccct ctctgatcgg
     2101 caataccgag tgcgctgcgc ctaagcggat gacaccatct tggacgcatc gccaacgcgc
     2161 cagctggcgc cctccattct cggagccgcg ccgcttccca atctagctcg cacgccgctt
     2221 gctctccgcc atcgtctccg gcgcgcgtcc gggggcacgt tataacgggg gcaggcgcgg
     2281 tgtcgcgccg gcgtgggagg gcagtgccgg tgccgctggg cagcggagcc gcagggtcgc
     2341 gcccgttgct gcgcggccgc caaattcaat atctgaatca cgcggaggag ctcgggcgag
     2401 gctcgattga ggtggtacct gtcactcgcc gaggtgttcg gggaagcggg cgccgtgggt
     2461 ggcggcgagc agctggatgc gggcgagttg gagcgaaagg cgggccccaa cgaggttgac
     2521 gcgcgcggtt tgttcgcgcg attgagcctc aatcagttct tctatcaggg gggacacgcc
     2581 ttgggcgtag cgctcttggg cgaggttcaa gttgagggtt gcgctgcgta ggatgtgctc
     2641 cgcgcgaacg tgcatggact cagcggcgcg ggcagcaatg gcggcttggg tggcttcggc
     2701 gcgcacggtg agaagctgag cgtgaagggc ttgttcggcg ctgtcgcgtt cggattcggt
     2761 gactcggact cgagggctga ccgtgaagtc gagtacgggg acgctgagca gggctccggc
     2821 ttggacggtc tcggagaagc cggcgagccc gtagcccgag atgacgtctt gtcggttgat
     2881 aacgccgttc gcgagcacag acagtacggg gcgggcgagg gcacgcgtcg cgtcgattcg
     2941 ggccctatcg tgttccaggt gggcacgggc ggcctggatt tccggtcgat ggatctgggc
     3001 cgcggcggcg gcgcgttgca gatctgggtc tacgccaagg ttcgtgggat ccagcgcttg
     3061 gagcgccaag ggtgtggacg gatcgagccc gagcgaagcg gccagtgcgg ctaggtcttg
     3121 cgccacgcgc gcttcggccg cttcgatttc gaagccggcg atgtcgatct cgagttgggc
     3181 tcgattctgg tcgagggcag ggcgtagacc acgagcgact aactcgcgcg tgacgttggc
     3241 ttggagttcg cgctgacgga gcgtgacgcg tgcaacttcc acgagctcgc gatcactcaa
     3301 cacgagcagg tatcggttgg ccgcgtccag cgcgacttgg tatctgagca cgttggcgtc
     3361 agcggttccg gcggcgtggg cctgctcggc ggcgcgaacg gcattggcgg tgcgcccgaa
     3421 gtcatagacg agggcttgcc cggtcagcga ggcttcggcc acaccgtaag tgctctgtac
     3481 acgaacgtcc ggcaatacgg ggcgattggt gtggccgacg gcgcccgtga gcgtgccact
     3541 cacggtgggg agataggcgg cgcgggtcac gtcgatttga gcctcgagtc cacgcactcg
     3601 cgcgtcggcg ccgcgcacgt tggggtgctg ccggacggcg atgccgacgg cttcacgcaa
     3661 ggagagcgcg cgcggggtag gcggcgcttg cgcctcgacg ataggtgccc ttgaaggcgg
     3721 tgcagttgga gtttgagccg cagcttcttg cgccacagtc aacgccagca tggcggtcac
     3781 gaccaagagc aggagttgcc gcatcatgct ttcagagacc gcgcagggtg aagggtgtca
     3841 atcatgccct cgtagagcgc ggcgcgttcc gagagagaga gtccccagcg attccaatgg
     3901 aacatggtca gaaacgccaa gatcgcgcgc gggccttgcg tgagtgaacc cagatgaagt
     3961 gcgttgttta gtgcgttcgc gaatcgcgta ttggttctct cgtagctcag gagcaggcgc
     4021 cggtccggct ccggcacacg ctcggcgagt tgtctcaagc caaaggactc aaactcccga
     4081 acacgagcac tcccggtcgg ttccactgcg ttatgcattc ccgagccgtg caggcgttga
     4141 agattgcacc aaacgtccca ggcctcatcg cggctctccg tggcgcgcga aaacaagtca
     4201 ctcagcaccg cgagcgatag cgtgggcgcg gtgagcgcgc tttggccttg gcgttgccgt
     4261 gggaaccacc gcacgaaagc ggccgagtcc gcgtcgaaga aggcatgcac gtgcggaacg
     4321 aggcttgggc tccacttgta tttctccggc tcgtaaatgg ccggggccca gccctgaatc
     4381 gccccttggc cgccgagttc gtgcaggcgc atctgcaggc gagtcaacgc ggaccgctcc
     4441 acgaaacgaa aacgcagtcg taaccccggc ggcttgcgca tgaaaaagaa ccgcttcaac
     4501 tcgccctgtg cgcgccaacg tttgatctcg ggcccgagcg ccgcgaatag cgcgagggct
     4561 tggtcccgcg cggcgacccg atcagagctg agctgcacgt tggcttggaa ccaaccgttc
     4621 gtctgcttcc gagtgcgc
//
LOCUS       DASTKU010000840         7231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1952001, whole genome shotgun sequence.
ACCESSION   DASTKU010000840 DASTKU010000000
VERSION     DASTKU010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7231
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952001"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VFQ61_29420"
     CDS             <1..1094
                     /locus_tag="VFQ61_29420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET9958663.1"
                     /translation="RERGGVEGRRGANDFGAQRMTVEYAQPGTAPRVSVLLPVYNAEA
                     TLEAALLSILRQTLPDFECVAVDDGSSDGSFAVLERFARLDRRVRAVRAPHGGLIAAL
                     NLGLAQCRGQFVARMDADDAMHPERLAAQSAALERRADLAGLGCHVRLFPARQLKPKR
                     LAYADWLNAIQDEEQVFAARFIECPLAHPTWFMRREVMVSYGYRDRGWPEDYDLVLRL
                     LAEGHRLGIVPRQLLFWRDSTARLSRTHPAYAQSRFVSCKAEFLASSWLAEQDRYVLW
                     GYGDTGRELRRALLVYGKTPRAIVELHPGRLGQRIHGAEVIPPSGLARLAKSVPIVVS
                     VAGATARGLIREQLHSLGFEPERHYVFAA"
     gene            complement(1142..2467)
                     /locus_tag="VFQ61_29425"
     CDS             complement(1142..2467)
                     /locus_tag="VFQ61_29425"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HET9958664.1"
                     /translation="MSTRPSLSSPFSLGVATSSYQIEGAVNEDGRGPSIWDVFSHTPG
                     KVLGGHTGDRACDHYHLLEQDLELLGWLGVDAYRFSIAWPRVLPNGRGQINSAGLDFY
                     ERLVDGLLARGIAPCATLYHWDLPAALPGGWLSRDTVEAFGEYAAVVADRLGDRIAMW
                     LTHNEPWCQAFLGYETGMFAPGHQRLQDALTCAHHLLVSHGVAVNALRSRVKTPVGIA
                     LNFLPAHPASSNPEDIAAAERVDSNFNRWFVEPVAGRGYPQPMVDWYGANMPEIRSGD
                     FETIAVPIDVLGVNYYERTVVTHDPAGGSLQRRSVRSELPRTADREIYPPGLSETLRR
                     LHHEYGFQRLVITENGAAFDDELASDGRVHDSGRVQFLESHLREVETLRAEGVPVEGY
                     FAWSLMDNFEWAAGYSLRYGIVHVDFETQKRTPKDSAVYFREVCKQRRP"
     gene            complement(2510..3013)
                     /locus_tag="VFQ61_29430"
     CDS             complement(2510..3013)
                     /locus_tag="VFQ61_29430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9958665.1"
                     /translation="MRILGICGSLQARSANRVLLESARRLAPAGVEVQLYDALGTLPH
                     FNPDWPSEDQPAVMALREHLANSLGVLIASPEYGFSLPGALKNAIDWLIGSGELERKV
                     VAITAAVPVAERGRMGLQALKQTLGAVSARIVWDEPIARGADFEPKLADLMGRLVQAA
                     RDEAPPL"
     gene            3383..4702
                     /gene="xylA"
                     /locus_tag="VFQ61_29435"
     CDS             3383..4702
                     /gene="xylA"
                     /locus_tag="VFQ61_29435"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HET9958666.1"
                     /translation="MSEPAFPGVEPIRYQGPEGTNALGFRYYDKDRIVRGKRMEDHLR
                     MAVCYWHTFCWNGFDVFGAGTFNRAWHRDGDAVELAKQKAAAAFELFEKLGVPFFCFH
                     DRDVAPEGRTLAESNEILDRMVDVLAANMAKSKIKLLWGTANLFSHPRYMAGAATNPD
                     PEVFAYAAAQVKKALEVTHKLGGENYVLWGGREGYETLLNTDMGHEMDQLGRFMNLVV
                     EHKHKIGFKGPLLIEPKPREPTKHQYDYDTATVYAFFQRYGLKSEDFKVNIEANHATL
                     AGHSFEHEVAYAIAAGIFGSIDMNRGDPQLGWDTDQFGNDLAELSLVMAQILKAGGFT
                     TGGFNFDAKVRRQSIDGQDLFHGHVGGMDNLARALLTADKMLSDGKLQALLDERYAGW
                     KEPLGKEILSGKLSLEQISERALSQKLDPKPRSGRQEMLENLVNRYL"
     gene            4887..5507
                     /locus_tag="VFQ61_29440"
     CDS             4887..5507
                     /locus_tag="VFQ61_29440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958667.1"
                     /translation="MAPNDPQPHDPADDPYAAVLDYQRTDHAGDRYYSSEPQHYERPL
                     ELERHPSRQAYFYRAMTREEAQGWLHPTRYAMPDPSGHHPWASNRDYSAGYLTRRHGY
                     THLVEVYAPDFVPQMMSIGFVSGKAEAGDISWGIGGTPSNGWRGSKTTNKVLESAYAD
                     LKGRKVSPKEVRGRERGMANELAPYLFKDSIRSVRVVDLRSQKPGR"
     gene            complement(5511..6008)
                     /locus_tag="VFQ61_29445"
     CDS             complement(5511..6008)
                     /locus_tag="VFQ61_29445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958668.1"
                     /translation="MAGVIRPSFELESPLDADTIVELVHRRLVDRESPICGVTAAERI
                     ELHVPSSRQHLWSPQLVIDLHEEEEGRTAVYGRFGPHPHVWVLYVGVLAAAAFACLVG
                     GSFGVAQLAMEETPTALWSLPAAAAVVVVLYLMAVAGRSLSAEQMSELRSFFEGALQD
                     RPVQV"
     gene            complement(6163..>7231)
                     /locus_tag="VFQ61_29450"
     CDS             complement(6163..>7231)
                     /locus_tag="VFQ61_29450"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET9958669.1"
                     /translation="KGSLSAAQAATAIAEGWAEARPGDEIECLPMADGGDGFGVVMGE
                     LLGAELQTCATVDCAGRAREAQWWFDQASKTAVFEAAQVNGLALLPTGMFHPFSLDTS
                     GIAAVMREIASRRARRLYLGIGGSATNDGGFGLARALGWSFRDANGIALQQWTELHRL
                     ERVDAPDERLQFDELVIAVDVANPLLGPNGATRVYGPQKGLQGSDLDRAEACLARLSS
                     VISSDLGAEPGAGAAGGLGFGLRVFCGGEFQSGAALFARTANLEQRLARADLVITAEG
                     AMDAQTFMGKGVGAIAGLAARAQIPCLCLVGRLFVEPSHFPWPKFQAFGIVPELATFE
                     QSWLEPAKFLRLLAAQVGSRF"
BASE COUNT         1415 a         2359 c         2207 g         1250 t
ORIGIN      
        1 ggcgtgagcg gggtggcgtt gaaggtcgac gcggtgccaa cgattttggc gcgcagcgaa
       61 tgacggtgga gtacgcccaa ccaggaacgg caccgcgcgt ctcggtgctc ttacccgtgt
      121 acaacgcgga agcaacgctc gaggcggcgc tgctcagcat ccttcgtcag actttgccgg
      181 atttcgagtg cgtcgccgta gacgacgggt cgagtgatgg gagcttcgcg gtgctggaac
      241 gatttgcgcg gctggaccgg cgcgttcgcg ccgtgcgggc gccgcatggc gggctaatcg
      301 ccgctctgaa cctgggtctg gcgcagtgtc ggggccagtt cgtggcacgc atggacgcgg
      361 acgatgcgat gcaccccgag cggttggccg cgcagtctgc cgcgctcgaa cgacgtgcgg
      421 acttagccgg gctcggttgc cacgtgcggc tcttcccggc gcgacagctc aagcccaagc
      481 ggcttgcgta cgcagattgg ctgaacgcga tccaggacga agagcaggtt ttcgccgctc
      541 gcttcatcga atgcccgttg gcgcacccga cttggttcat gcgccgtgag gtgatggtca
      601 gctacggcta ccgtgatcgc gggtggcccg aagactacga tttggtgctg cgcctactcg
      661 cagagggtca ccggctcggg atcgtcccgc gtcagctgtt gttttggaga gacagtaccg
      721 cgcggctttc acgtacccat cccgcttacg cacagagtcg gttcgttagc tgcaaggcgg
      781 agtttttggc tagctcatgg ctagcagagc aggatcggta cgtgctctgg ggctatggag
      841 acacgggccg ggagctgcgg cgagcgctac tcgtatacgg gaaaacaccc cgagccatcg
      901 tcgagctgca cccgggtcgc ctcgggcagc ggatccatgg cgccgaggtc attccacctt
      961 cgggcctggc gcggctcgcc aagagcgttc cgatcgtcgt ttcggtcgcg ggggccacgg
     1021 cgcgtggcct gatccgggag caactgcact cgctgggctt cgagcccgag agacactacg
     1081 tctttgctgc gtgatccgcg tttgggagct cagaactcat cccagtcatt gagataggtt
     1141 cttagggtcg acgctgtttg cagacctctc gaaaatagac cgcgctgtct ttcggagtgc
     1201 gtttctgagt ctcgaagtcg acgtggacga tgccgtaacg cagcgaataa ccggcggccc
     1261 actcgaagtt atccatcaac gaccaagcaa agtagccttc gacgggaacc ccttcggcgc
     1321 gcagagtttc cacctcgcgc aagtgcgatt ccagaaactg gacgcgcccg gaatcgtgca
     1381 cccgcccgtc gctcgcgagc tcgtcgtcga atgcggcacc attctcggtg atgacgaggc
     1441 gctggaaacc atactcgtga tgcaagcgac gcagcgtttc cgacaatccg ggcgggtaga
     1501 tctcacgatc cgcggtgcgc ggcagctcgc ttctcacgct ccgccgctgc agagagccgc
     1561 ccgctggatc gtgcgtcacg accgtgcgct cgtagtagtt gacgcccagt acgtcgatcg
     1621 gcaccgcgat cgtctcgaag tccccacttc ggatctccgg catgttcgcg ccgtaccaat
     1681 ccaccatggg ctgtgggtag ccgcgccccg cgaccggttc cacgaaccaa cgattgaagt
     1741 tggagtcgac ccgctccgcg gcggcgatgt cctcggggtt ggagctcgcc ggatgagcgg
     1801 gcaaaaaatt cagagcaata ccgaccggcg ttttcacccg actacgcaga gcgttaacgg
     1861 ccaccccgtg cgagacgagc aaatgatgag cgcaggtgag cgcgtcctgt aggcgctggt
     1921 gacctggcgc gaacatgcct gtctcgtagc cgaggaacgc ctgacaccaa ggctcgttgt
     1981 gggtaagcca catcgcgatg cggtcgccca agcggtccgc caccaccgcc gcatattcgc
     2041 cgaacgcctc gaccgtgtcg cgcgacaacc aacctccggg caacgccgcc ggcaagtccc
     2101 agtggtagag cgtcgcgcac ggcgcgattc cccgcgcaag caggccgtcc accaaacgct
     2161 cgtagaagtc tagccccgcg gagttgatct gcccgcgccc gttcggcagc acgcggggcc
     2221 acgcgatact gaaccgataa gcatcgaccc cgagccagcc cagcagctcc aggtcttgct
     2281 cgaggagatg atagtgatca cacgctcggt caccggtatg accgccgagc accttgccag
     2341 gagtgtggct gaatacgtcc caaatgctgg gaccgcgccc gtcctcgttt acggcgccct
     2401 cgatttggta agacgaggtg gcaactccca aagagaacgg cgaactcaaa gaagggcgtg
     2461 tcgacataaa tgttcggccg aggaggatac acgaagcccc ggccggctgt cacaggggcg
     2521 gggcttcgtc gcgcgccgcc tgcactagac ggcccatcaa atccgccagt tttggctcga
     2581 aatcagcacc acgggcgatc ggctcgtccc acacgattcg agcgctcacg gcgcctaacg
     2641 tctgtttcag cgcctggagc cccatccgcc ctcgctccgc aacgggtacg gccgccgtaa
     2701 tcgcgaccac cttgcgctcg agctcaccgc ttccaatcag ccaatcaatc gcattcttca
     2761 gcgcaccggg caggctgaag ccatactctg ggctcgcgat caagacgccc aggctgttcg
     2821 cgaggtgctc gcgtaacgcc atgaccgcgg gctgatcctc agatggccaa tcgggattga
     2881 agtgtgggag ggtgccgagt gcgtcgtaga gttgcacttc cacgcctgcc ggggccaggc
     2941 ggcgcgcgct ctccagcaaa acacgattcg ccgatcgtgc ctggagactg ccgcagatgc
     3001 cgagaattcg caccggccca gactagctcc gttttcctgc ggagtctgcc tacccaggtc
     3061 gcgccgctcg cgctgagcca agactgcgtg cggcaaatgc gagacatgcg accacaatgc
     3121 gatatgtggt gaatcaagcc tgttttgcgc ggtgcataca gttgtgtctc ggggcgcgcc
     3181 gaattgcgac gcagcgcgaa ggacacgctg cgcttgaaaa aggccggccg gaggcacttt
     3241 cgcgaaggcg gcgcgcgggg tacagtatgc gcgctcgaca ttcgagctcg ctcgcgtccc
     3301 tctccccgtc gtgtttcttt gggggcggcc gagcgctgat acccaatcga caagccttcg
     3361 aaaaactgga tcaaggagcg tcatgtcaga acctgcgttt cccggcgtcg agccgattcg
     3421 ctaccaaggc ccggaaggga ccaatgccct cgggtttcgc tactacgaca aggaccgaat
     3481 cgtgcgcggc aaacgcatgg aagatcacct gcgcatggcc gtttgctact ggcacacgtt
     3541 ctgttggaac ggcttcgacg tgttcggtgc ggggaccttc aatcgcgcct ggcaccgcga
     3601 cggtgatgcg gtcgagctcg ccaagcaaaa ggcggcagcc gcgttcgagc tgttcgagaa
     3661 gctcggtgtt ccgttcttct gctttcacga ccgtgacgta gcgccggaag gccgcacgtt
     3721 ggccgagtcc aacgagatcc tggaccgcat ggtcgacgtg ttggccgcca acatggccaa
     3781 gtccaagatc aagctgctgt ggggcacggc gaatttgttc tcgcatcccc gctacatggc
     3841 gggcgcggcc actaaccccg atccggaagt gttcgcgtac gccgccgctc aggtgaagaa
     3901 ggccctggag gtcacgcaca agctcggcgg tgagaactac gtgctgtggg gcggccgtga
     3961 gggttacgag acgctgctca acacggacat gggccacgag atggatcagc tcgggcgctt
     4021 catgaacctg gtcgtggagc acaagcacaa gatcggcttc aagggtccgc tcctgatcga
     4081 gccgaagccg cgcgagccca ccaagcatca atacgattac gacaccgcca cggtgtatgc
     4141 gttcttccag cgctacggcc tgaagagcga ggacttcaag gtcaacatcg aggccaacca
     4201 cgcgacgctc gcgggccaca gcttcgagca cgaagtcgct tacgcgatcg ccgccggcat
     4261 cttcggcagc atcgacatga accgcggcga tccccagctc ggttgggaca ccgaccaatt
     4321 cggcaacgac ttggcggagc tcagcttggt gatggcgcag atcctcaagg cgggcggatt
     4381 cacgaccggc ggcttcaact tcgacgccaa ggttcgtcgt caaagcatcg acggacagga
     4441 cctgttccac ggccacgtcg gcggcatgga taacctcgcg cgcgccttgt tgactgcaga
     4501 caagatgctc agcgacggca agctccaggc gctgctcgac gagcgttacg ccggctggaa
     4561 agagccgctc ggcaaggaga tcttgagtgg caagctctcg ctcgagcaga tctccgagcg
     4621 tgcgttgtcg caaaagctgg atccgaaacc gcgctcgggt cgccaagaga tgctcgagaa
     4681 cctggtcaac cggtacctgt gagacgcacg cgcgtgcggg tttgacgccg gagttttacc
     4741 cggtttttgc ggttgattgc tgattggagc cacgtcgcgt acgcgcgacg tggctccttc
     4801 gttttgtggt ctcgacgaga agtgcgcgtc gctgcactgc ggtcctacgc gggccgtgga
     4861 gcgcgagcta acactttcga acgcgcatgg caccgaacga tccgcaacca cacgaccccg
     4921 ctgatgaccc ctacgctgca gtgctcgatt accagcgcac cgaccacgcc ggagaccgct
     4981 actacagctc cgaaccacag cactacgagc gtcccttgga gctcgaacgt cacccgagcc
     5041 gacaagcgta cttctaccga gccatgacgc gcgaggaggc gcagggttgg ctgcacccga
     5101 cgcgctacgc gatgccggat ccgagcggac accacccttg ggccagtaac cgcgactatt
     5161 cagctggata tctgacccga aggcacggat acacgcatct cgtggaggtc tacgcgcccg
     5221 acttcgtacc tcagatgatg tccatcggct tcgtctcggg gaaagcggaa gcgggcgaca
     5281 tctcgtgggg aatcggcggc acgccctcga acggctggcg cggaagcaag acgaccaaca
     5341 aagtcttgga gtccgcgtac gcagatctca agggcaggaa ggtatcaccc aaagaggttc
     5401 gcggccgcga gagaggcatg gccaacgagc tcgcgccgta cctcttcaaa gactcgattc
     5461 gatccgttcg ggtcgtcgat ctgcgctcgc agaagccggg acgttagccc tcaaacctgg
     5521 actgggcgat cttgcaacgc cccttcgaag aagctgcgca gctcggacat ctgctccgca
     5581 ctcaagctgc ggcccgcgac cgccatcaag tagagtacga ccacgacggc agcggcggcc
     5641 ggcaatgacc acaacgcggt gggcgtctct tccatcgcga gctgcgccac tccgaagctg
     5701 cctcccacca agcaagcaaa agccgccgcg gccagcactc ccacgtaaag cacccagacg
     5761 tgcggatgcg gcccgaatcg accgtagacc gccgtgcgcc cctcctcctc ctcgtgaaga
     5821 tcaatcacga gctgcgggga ccacaggtgt tggcggctcg aaggcacgtg cagctcgata
     5881 cgttcggcgg cggtaacacc gcaaatcggg ctttcacgat ccaccaatct acggtgtacc
     5941 aattcgacga tcgtgtctgc gtcgagcggc gattcgagct cgaacgaagg tctgatcaca
     6001 ccagccatac atcgagtatg ccgagccctc cgcgagaaat ctcgtcgaaa cccggcgccg
     6061 cggcagattc cctatttgca ttgaactagt tgcacagcac gagaattcgc tgccacccat
     6121 gtcgccagcc cacacaccac accacgcttt tcgtggaaag attcagaatc tgctgccgac
     6181 ctgcgcggcc agaagccgca agaatttggc cggttcgagc cacgattgct cgaacgtcgc
     6241 cagttcgggc acgatgccaa aagcctggaa cttcggccac ggaaagtgtg atggctcgac
     6301 gaaaaggcgg ccgacgagac atagacaagg gatctgcgcg cgcgccgcca agccggcgat
     6361 ggcgcccact cctttaccca tgaaggtctg cgcgtccatg gcgccttccg ccgtaatgac
     6421 gaggtccgca cgggccaggc gctgctcgag atttgcggtc cgcgcgaaca atgcggcacc
     6481 agactgaaac tctcctccgc agaacacacg caaaccgaac cctagccctc cggcggcacc
     6541 tgcacccggc tccgctccga gatcgctgga gatcacgctc gataaacgag cgagacacgc
     6601 ctcggctcta tccaagtcgc taccctgcaa gcccttttgc ggaccgtaga cgcgcgtggc
     6661 gccattcggg ccgagcaacg gattggcgac atctacggcg atcacgagct cgtcgaattg
     6721 caaccgctca tccggcgcgt ccaccctctc gagccgatgg agctccgtcc actgctgcaa
     6781 cgcaatcccg ttcgcatcgc ggaacgacca ccccagagca cgggccaatc cgaatccgcc
     6841 atcattggtc gcactccccc caattcccag gtagagccgg cgcgcacgcc gacttgcaat
     6901 ctcccgcatc accgcggcga tccccgaagt gtctagtgag aacgggtgaa acatgcccgt
     6961 cggaagcaaa gccaacccgt tcacctgcgc cgcttcgaac acggctgttt tcgaagcctg
     7021 atcgaaccac cattgggctt cccgcgcgcg cccggcacag tcaaccgtgg cacatgtctg
     7081 cagctctgcc cccagcaact cccccatgac gacgccaaag ccgtctccgc cgtcggccat
     7141 gggcaaacat tcaatctcgt ctcccgggcg agcttccgcc cagccctcgg cgattgccgt
     7201 ggccgcttgc gcggcgctga gggagccctt g
//
LOCUS       DASTKU010000841         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_610916, whole genome shotgun sequence.
ACCESSION   DASTKU010000841 DASTKU010000000
VERSION     DASTKU010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610916"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..664)
                     /locus_tag="VFQ61_29455"
     CDS             complement(248..664)
                     /locus_tag="VFQ61_29455"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HET9958670.1"
                     /translation="MATRKKRTTSETASARRPRKKTGSRKKAGSRKKASKKANKKRAA
                     RRAARPASGRTKKASKRAAKSGARKYGDKAQDKVARSMREMKQGKLRSGRSGKKVTSR
                     QQAIAIGLSEARREGGKVPPPPARSTSRKKKTSSRR"
     gene            837..2309
                     /locus_tag="VFQ61_29460"
     CDS             837..2309
                     /locus_tag="VFQ61_29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B6"
                     /protein_id="HET9958671.1"
                     /translation="MYRFSMLSVALMSAMFGCSSEQSKGTASVELSANAPPGTRGAFS
                     EAPPNAPPPPASCGDMNGGGDQHVDFTALRAAKIAEKPDVMRRQSDLLASRYDLSDRP
                     ASGVHMTRGKPVQAGVRVLLPGVTWAELAAMSPEEIKANDLFPFGFFALPHPKQPEGG
                     MLFPQSTIDETIRQTGRDLTRFDLDFDIPEHLIAEFPPPIFLTTRPDLGDVSQGQLVT
                     IDNFYDLFKCILNPKQLDGLRLLLTPFPQQQFNAIDDRRSDHPSLGVACFDCHVNGHT
                     NAATHLVGDIRPQEQRHRVDTVSLRGVNVQRTFGSQRALMSVEDFTEFEQRAAYFDGD
                     PVLATKKGVNPLDRGLQVQHMAEFQKLLDFPPAEKLGLDGKLDPEKATVAELRGQEIF
                     FGKGKCATCHTPPYYTDSLMHDLELERFYEPRTINGILATAEGPIKTFTLRGLKDSPP
                     YFHDGRLLTIEDTIEFFNLILGLGLTPREKADLLAFLYVL"
     gene            complement(2465..3124)
                     /locus_tag="VFQ61_29465"
     CDS             complement(2465..3124)
                     /locus_tag="VFQ61_29465"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HET9958672.1"
                     /translation="MNRKTLWFAAALTLSFAGCKNEPVPGDRTTGTGGGATDNGWTAG
                     MTGTGATGTVTPPTGSGAENGTSGTVAARTAKATFESAPGVSLKAEATLEEQQKGVRI
                     VINVEQGPPGDKAVHVHQKGDCSDIPGKSMGEHFAPHGETHGLPSSPQHHPGDLGNIT
                     LDKDGKGKLEIVTSAGNLIPNDPDSFAGRAIVIHTSNDKGTPPSGDAGTPIACAVIRT
                     P"
BASE COUNT          558 a         1025 c         1011 g          706 t
ORIGIN      
        1 gatccaggta ggggcacgcg cgctgaaggt ctggaagctc gcgcccggtg accatcacga
       61 cgtgacgacc gctcgcccga acgcgctcga tggcggacac ggtttctgcg tccatcgctc
      121 cgtggtgggc cagggtgcca tcgtagtcgc atgccagaat tcgataacgc atgttcgtgc
      181 ctgtggacct tcacatgggg actggggacg cgcccgcgac gcaggcagcg ccttgactcg
      241 tgcgtactca gcggcgcgaa gacgtcttct tcttacgcga cgtgcttcta gccgggggcg
      301 ggggaacttt gcccccctcg cgccgcgctt ccgagagtcc gatcgcgatg gcctgctgac
      361 gactcgtgac cttcttcccg gagcgacctg atcgcagctt gccctgcttc atctcacgca
      421 tgctgcgcgc gaccttgtct tgggccttgt caccgtattt gcgagccccg gacttcgcgg
      481 cgcgcttgga agctttcttg gtgcgcccgc tggcggggcg agcagccctc cgagcagctc
      541 tcttcttgtt ggccttctta gacgcctttt tccgcgatcc tgccttcttt cgagatcctg
      601 tctttttcct gggccttctc gcactagccg tttcgctagt cgtgcgcttc ttgcgtgtcg
      661 ccatatcgcg ttctcccgtg tgtcgcggcg cagcaactaa cgtgccgatt cgctacccgg
      721 cgcgtcgggc tcgaagggaa tcttggatgc ccattgtggc aagctggtga tttgcggcgg
      781 tctggcgcag ccgttgcaaa gcgcaccgcg gccactcagg ccggaggagc gatcgcatgt
      841 accgattcag tatgttgagt gtcgcgttga tgagtgcaat gttcggctgc agcagtgagc
      901 aaagcaaggg gactgcctcg gtcgaactct cggcgaacgc gccgcctggc acacgtggag
      961 cattctcgga ggcgcctccg aacgctccac cgccgcctgc gagctgcggc gacatgaacg
     1021 gtggagggga tcaacacgtc gatttcacgg ccttgcgcgc cgcgaagatc gcggagaagc
     1081 cggacgtgat gcgccgccag tcagatctgc tggcctctcg ttacgacttg agcgatcgac
     1141 cggcgagcgg agtacatatg acgcggggca agcccgtaca agctggggtg agagtgttgt
     1201 tgcccggcgt tacttgggcc gaactcgcgg ccatgtcgcc ggaggaaatc aaggccaacg
     1261 acctgttccc atttggcttc ttcgcgctgc cccatccgaa gcaaccagaa ggaggcatgc
     1321 tcttcccgca gtcgacgatc gacgagacga tacgccaaac cggccgcgat ctcactcgct
     1381 tcgatctcga tttcgacatt ccggagcacc tgatcgcaga gtttcccccg ccgatattcc
     1441 tgacgactcg gcccgatctc ggcgacgtat ctcaagggca actcgttacg atcgacaact
     1501 tctacgattt attcaaatgt atcctcaacc cgaagcagct cgacggcctg cggctcctcc
     1561 tcaccccgtt tcctcaacaa cagttcaacg ccatcgacga ccggcgatcg gatcatccga
     1621 gcctgggagt tgcttgcttc gactgccacg tcaatgggca caccaatgcc gcgacgcatc
     1681 tggtcgggga tattcgtccg caagagcaac gccatcgcgt ggataccgta tctttgcgcg
     1741 gggtgaacgt gcaaaggacg ttcggttcgc agcgcgcgct gatgagcgtc gaggacttca
     1801 ccgagttcga gcagcgagcg gcttacttcg atggtgaccc cgtgctcgct accaagaagg
     1861 gcgtaaaccc gttggatcga ggcctccagg ttcagcacat ggccgagttc cagaagctgc
     1921 tggatttccc tcccgcggag aagctcggtt tggacggcaa gctcgacccc gaaaaggcca
     1981 ccgtggctga gctccgggga caagagatct tcttcggaaa gggcaagtgc gcaacttgcc
     2041 acacgccccc ttattatacg gacagcctga tgcacgactt ggagctcgag cgcttctacg
     2101 agccccgaac catcaatggc atcttggcca ccgcagaggg cccgatcaag accttcacgt
     2161 tgcgtggcct gaaggattcg ccgccctact tccacgacgg tcgtctgctc acgatcgagg
     2221 atacgatcga gttcttcaac ctaatcttgg ggcttggcct cacgccgcgt gaaaaggcag
     2281 acttgctggc gttcttgtac gtgctgtaaa gggtacgagc cgcaactaag tagatcgata
     2341 aggcttagat cgataggcct taaatcgacc gggcttagat cgacaaggcc ccgcagcacc
     2401 gcctgcgggg ccttgaatgg cgcgtacgtg gctcaccggc gcttcgctgg tttcacgtgc
     2461 gctgctaggg cgtgcggatt acagcacacg cgatgggtgt gcccgcgtcg ccgctcggtg
     2521 gagtgccctt atcgttgctc gtatggatga cgattgcgcg tccggcaaag ctatccgggt
     2581 cattggggat taggttaccc gcgctggtga cgatctcgag tttgcccttt ccgtctttgt
     2641 ctaaggtgat attaccgaga tcccccggat gatgctgcgg tgagctcggc aagccgtggg
     2701 tctccccgtg tggcgcgaag tgttcgccca tactcttgcc agggatatca ctacagtcgc
     2761 ccttttggtg gacgtgaacg gccttgtccc cgggtggtcc ctgctcgacg ttgatcacga
     2821 ttcggacgcc tttttgctgc tcctctaagg ttgcctcggc cttgagcgac acacctgggg
     2881 cgctctcgaa cgttgctttc gcggtccgcg cggccaccgt gccgctcgtt ccgttctccg
     2941 cgcccgagcc cgtcgggggc gttaccgttc ccgtcgcgcc cgtgccggtc atgccggcgg
     3001 tccagccatt gtcggtggcg cctccgccgg ttccggtggt tcggtcgccg ggaacaggct
     3061 catttttgca accagcgaag ctcagcgtga gtgcggccgc aaaccaaagt gtcttgcgat
     3121 tcatgcgtgc atactccagt tccatgcgtt ggcccggaaa ggaccgggtt tcgacgtcga
     3181 agcctctgaa cgggctgacg ggtgctgacg ggtgcgagcc gcgtgccaag accccgcgcg
     3241 ctgaggcggt gcgcgtgatg ggtgaggcgt tcgggcgttt tgcagcaatc gttcgttcga
//
LOCUS       DASTKU010000842        10526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1281775, whole genome shotgun sequence.
ACCESSION   DASTKU010000842 DASTKU010000000
VERSION     DASTKU010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10526
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281775"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VFQ61_29470"
     CDS             <1..1022
                     /locus_tag="VFQ61_29470"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HET9958673.1"
                     /translation="LMDQQAALRWVQDEIGAFGGDPSNVTIAGGSAGGVSVCAHLVSP
                     LASGLFAAAVIQSGACYAAPRDASETQGLSFATAAGCPDATSMDCLRQKSAAELLAVD
                     SFSFGNFPVRGDALLPEGPGAAVEAGRFARVPVMIGGTHNEARAGAAAQFPMSEAEYI
                     TWLTDTFGDRAQSVLQLYPVTPNQDAFYPFSDVVNDSRLGGISSCWTHRIARAFARQV
                     ATFEYEFNDASAPIPTWVAPPAGLVLGASHTSDEAYWFRRPLDTIEPLDGAQRRLSRR
                     MIRYLGAFSDRHDPQVPRESHWPRVDASQSVMHFGSSAGPEFVGVKTDFEAEHHCAVW
                     EAMGR"
     gene            1574..3688
                     /gene="tssI"
                     /locus_tag="VFQ61_29475"
     CDS             1574..3688
                     /gene="tssI"
                     /locus_tag="VFQ61_29475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HET9958674.1"
                     /translation="MADRFHITLSSPLGEELLFQSLNGWEELSQPFNYELMLVTRERK
                     VRSNELLGQIIGVHVELPAGGTRHYGGHVTRFGLVKVVGSWLHYGVTVRPWLWLLSHS
                     SNCRIFQNQSVVEIVKAVLQAHGFSDVDERLMDPHPPHEYVVQYRETDLQFVSRLLES
                     EGIYYFFRHEESKQVLVLADAFSAHEPAPGCPPLPFMPAGGRRDHHMEYVSEWQSVAR
                     LQTAACALSDFDYKKPRVQLLVSSQEAADSGLEGFEFYDYPGAFLERADGEHQAKLRV
                     QECQALREVVEAEANAAALFSGSLFDLTDHPDDEQNRQYLVVGNRFRLTEYERVSGSQ
                     ASGADQQTYQSNLLLIPANHPFRPERKTPKPVIAGPQTAIVVGPSGSEIWTDELARVK
                     VKFHWHREDPANETSSCWIRVAQSWSGAGFGAQFVPRMGHEVVVEFLEGDPDRPLITG
                     SLYNGIDNPPFDEPTQSGVKSRSTKQGWPANANEIRFEDLKGSEEFYVQAEKDLEVLV
                     KNNETRQTGVDRSTRIGANDALTVGVDRSGTIGANDSLKVGANSQTVIGGAQSVVVAQ
                     GRSITASAEVLNVGTRSKIVGTTESIRVGGARTEAVGGDAKLSIAGKAELSIGKDHKV
                     TVSGDRNESVGKNDSLKVEKKLEIEAGDEIVIRTGQASMTLKKNGDIVIKGKNLKLDF
                     DSKVNVKAGSDIALKGSKIGQN"
     gene            3728..4270
                     /locus_tag="VFQ61_29480"
     CDS             3728..4270
                     /locus_tag="VFQ61_29480"
                     /inference="COORDINATES: protein motif:HMM:NF040117.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6484 domain-containing protein"
                     /protein_id="HET9958675.1"
                     /translation="MNDDSSNDGAEDLEPSVDSTTFTATLGTVSAFPELARVTRIDPE
                     GYVWVALEDGGAERRARLALCGEGLSTDMTVAVRFERGDRRCPVIVGLVSDRLPPGED
                     VTAREEPAKPTTQGRDLTITPSLDGRRVQVRAEDEIVLECGQASITLRRNGRVVVRGT
                     HVETYSEGVNRIKGAQVRIN"
     gene            4279..5601
                     /locus_tag="VFQ61_29485"
     CDS             4279..5601
                     /locus_tag="VFQ61_29485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958676.1"
                     /translation="MSAPRRVRPPPFRWDFVEESFSEAEFLWGRRQSALESTEYVLSE
                     LSRFIEERLLGCLDGVMLAGEAAHERLLVPALLGEEPRLASVAAWCLASSGERGLSLV
                     AAVLEQAEEPSLQALRAGLELVMWPELGARLEGLRNAASPRVHAALLEVFAFQGRGAG
                     ATLNELVRHPDPMLSAAALRAARFDARPAAEIAVGQGLEHAAAVCQWAAIETGLVLGL
                     EAVRGLALESWKQPWRPEYHRVWALTAALNPKQSSLTAALGDEKGLPGAAWALGFAGT
                     RDAADHCVALMTEGRVPELALDSFCNIVGCDPVVERLAQAPAPPADSPETLDEDDLDA
                     DLRLAVEVARWVPDPAQVTAYWARRREQFSPELRYQRGRAVSSPSELRELLWDLPLGR
                     RHVTALELAARTCGVEQFNPRAWCREQRARLTQLTQATPETAPLPEPV"
     gene            5604..6644
                     /locus_tag="VFQ61_29490"
     CDS             5604..6644
                     /locus_tag="VFQ61_29490"
                     /inference="COORDINATES: protein motif:HMM:NF021456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HET9958677.1"
                     /translation="MFEVVNRTPFPAALVPFRDLAGRDYLGLVIKATFELEAAYKRPS
                     AASPGEIQIAEEQPPPLTSDLRNGKTPTASARVLAELTRPKLATDLVLVGHACTESPR
                     DPQVDVCLRVGHHAKLVRVFGERVWCRAVGEWVISDTLPFERMPLVYERAYGGADTSD
                     PAPVRHGYEPRNPAGTGFVAHGSSVRLEGLRLPNLEDPRDLIGSWKQRPNPAGFGYVD
                     AQSEWRSRYAGTYDDAWRTQRAPLLPLDFDDRFFNAAHPELIYPGYLQGGELVRVDGV
                     APRQTLCFEVPRLKFSAAASIHDDTLDAAPRLDTLWIDADAAKLVLLYRAAFPTPRSG
                     FGVRAVFVRRHA"
     gene            6641..7720
                     /locus_tag="VFQ61_29495"
     CDS             6641..7720
                     /locus_tag="VFQ61_29495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958678.1"
                     /translation="MSFRLVISGVGAVSSVGAGALQTCASIRAGLSGLREHPDFYPQP
                     PNPVIEPPERVSAGLVPLGLSLAARRERMLELAARAAHELITLAPMARGETERTELVL
                     CVASAAQGGFDAAEASELTSALGQRLGLQLERPLRTISQGATGVLRAVGEARTRIAAG
                     EVARVIVVGVESWVTLDALAALDEARRLRSARILDGLIAAEAAACVLLENAENAQARG
                     RTALAEFASVGFGREPNTLEGELNSSGVGLTEAVRAATRSFGRSNWPWLLSDMTGESY
                     GAYELGLLRARLGEQLGSTEVWHPADCTGAAGTGSAALGLVIAARALADGYAPADRVL
                     LCVGAQGEERAAGTLLACTQPGRRE"
     gene            7723..8220
                     /locus_tag="VFQ61_29500"
     CDS             7723..8220
                     /locus_tag="VFQ61_29500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4150 domain-containing protein"
                     /protein_id="HET9958679.1"
                     /translation="MPSTVVVNFRTVVHQGSGGVANAFPDVCKTPTPAGPVPIPYPNI
                     AMSSDTAQGAKSVNADGNPIAIQGSVFSQSTGDEAGSAGGVVSSCIRGKAEFVMYSFD
                     VKADGKGVARLGDLMLQNKGSAPNTPPFPEVQAPLPVLPPDPAELQDNDIVEIEVVTP
                     EEYEQ"
     gene            8237..9568
                     /locus_tag="VFQ61_29505"
     CDS             8237..9568
                     /locus_tag="VFQ61_29505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958680.1"
                     /translation="MPTRLQWVNYPDADKTIGAIHVRRANFIDSDVGTTGVMSTTLFV
                     RIRFQRSDAAGAATLTIRPDGGNAAYSAQERLGQPGVFAPGLTAATVVSAQGYARFRV
                     KLTPAGGDKYRLEGRSAAGTVRNYANEVETRRKLFYQIIRMREARGLAAGMTGRVERQ
                     FWRPDKKLYIKLAQYARGHTIRNMENFDDTDPAVEARVKTRARRAYDRSKAPFAFVAI
                     FVNKNCIPAREHQVVAANFTGAPFVLNTQRRIFSHADRAVRWFHSMTFTPAGGAPVNI
                     PRRAVRVTGLRALTINTSSLPAGAGTLEYEITIVDIEGMGLSLPTENLVTIASRFLDG
                     SPVPAATMAAVMVHEIGHKIGMVPGPQGDHDLDRQSTYYDGRGHSGPHCRHPAPLVPT
                     YNSAAPLVPAPTCTMFGDIRTNTTQFCTNCVRSVRKLDLDPDRKPGIATQF"
     gene            9771..10241
                     /locus_tag="VFQ61_29510"
     CDS             9771..10241
                     /locus_tag="VFQ61_29510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958681.1"
                     /translation="MTADFSKVVYGYQPSECTPRFTPAEPDGIKIAMPSQIQLSTAAT
                     VRGKPALPLCISMRFSALYLEQFDPVFRFIQVVVIDDAGKQAFAGGIFHDRLYRPSPP
                     SDIPEAELAKRIAYKYGTVNLLEFFALPEIEATYHIHVLLKEHRSNVITVQVEP"
     gene            10238..>10526
                     /locus_tag="VFQ61_29515"
     CDS             10238..>10526
                     /locus_tag="VFQ61_29515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958682.1"
                     /translation="MTQDRSRPGHSPPAAPAELPCFGACTEHPKLDVGIVGLRIATAG
                     VSGTREQPRWILHGVLRVARELAGPNPEELFARIVIVLRGASSERIQARHAF"
BASE COUNT         1845 a         3148 c         3554 g         1979 t
ORIGIN      
        1 gcttgatgga tcaacaggcg gcactgcgct gggtccaaga cgagatcggc gcattcggtg
       61 gtgacccgag caacgtcacg atcgcgggcg gttcagccgg aggagtttcg gtgtgcgctc
      121 atttggtatc gccgctcgcg agtgggctct tcgcggcggc ggtcattcag agcggagcat
      181 gttatgcggc gccgcgggat gcctcggaga cgcaaggctt gtcgtttgct acggccgcgg
      241 gatgccccga cgctacgtcg atggattgct tgcgtcagaa aagcgcggct gagctcctcg
      301 ccgttgattc attcagcttc ggcaattttc ccgtgcgtgg ggacgcgctc ttgccggagg
      361 gtccgggagc ggccgtcgaa gcgggccgct ttgcgcgcgt gcccgtcatg attggaggga
      421 cccacaacga ggctcgcgcc ggggcggcag cgcaatttcc tatgtccgag gctgagtaca
      481 tcacgtggtt gacggacacc tttggcgacc gggcccagag cgtgttgcag ctctatcccg
      541 tcacgccgaa tcaggacgcc ttctatccct tcagtgacgt cgtgaacgac tctcgactgg
      601 gcggaatcag cagctgctgg acccatcgca tcgcgcgtgc gtttgcgcgc caagttgcca
      661 ccttcgagta tgagttcaac gacgccagcg cgcccatccc cacctgggtc gcacctcccg
      721 ccggcctcgt actcggcgca agccacacgt ccgacgaggc gtactggttt agacgccccc
      781 tagacacgat tgagccgctc gacggtgccc aacgccggct atcccgccgc atgattcgat
      841 atctcggggc gttctccgat cgtcacgacc cgcaggtgcc ccgagagtcc cattggccac
      901 gcgtagatgc aagccagagt gtcatgcact ttgggtccag tgccggaccg gagttcgtcg
      961 gagtgaagac ggacttcgag gcagagcatc actgcgcagt ttgggaggcg atggggcgtt
     1021 gagggcagcg ggcgcctgtc gcacgcgtgc tcggacatcc ctacccggtc cgcacagact
     1081 tggctcgctc tctttctaac ccgcgctcgg tggctacgcg ggacgtgacg ccaccacacc
     1141 aatattcgtc ggtgctcacg atagttcttg ttttgcgtac gttgagcaac atcgccgaac
     1201 caagatccat ttccctgggc tacgcaaacc agcgttgcct attgaacgcc ccgctcgtca
     1261 gcacacgttc gatcgacttt ttcgagggtt ggggcttcgt gacgtgccgc ctgcgcggtg
     1321 ctggcgggaa acgggaggga cggtgtctgc gctcgggtcg acggcggaga tgggtcgcgc
     1381 ccgttacggg gggcggtcaa aaatcatggg gatgatgatg agcctacagg gcgttcatat
     1441 tgttcacatg gggggcggag gtcgcacggg gcggggcgta cgaattatca tgcgggtggt
     1501 ggggaggttg ggctagggtt gcgcgaacga gtagcggcgg cgcatcgcat ttgcgtttcg
     1561 acgggcgagt tctatggctg atcgtttcca catcacgctg tcttcgccgt tgggcgagga
     1621 gttgctgttt cagtctttga atgggtggga ggaattgagt cagccgttta actacgagtt
     1681 gatgctggtt acccgcgaac ggaaggtgcg gagcaatgag ctgttagggc agattattgg
     1741 agtgcacgtg gagttgccgg cgggcgggac gcgccactac ggcgggcacg tgacccggtt
     1801 tgggttggtg aaggtcgtgg ggagttggct gcactacgga gtgacggtgc ggccatggtt
     1861 gtggctgctt tctcacagct cgaattgccg tatttttcaa aatcagtcgg tggtggagat
     1921 cgtgaaggcg gtgctgcagg cgcacggctt tagtgacgtg gatgagcggt tgatggatcc
     1981 gcacccaccg cacgagtacg tggttcagta ccgagagacc gatctgcagt tcgtgagtcg
     2041 gctgctggag agtgaaggaa tctactactt ctttcgacac gaggagagta agcaggtgtt
     2101 ggtgctggcg gatgcctttt cagcgcacga gccggctccg gggtgcccgc cgttgccctt
     2161 catgccggca ggcgggcggc gcgatcacca tatggaatac gtgtcggagt ggcagtcggt
     2221 ggcgcggctt cagaccgccg cgtgtgcgct ctcggatttc gattacaaga aaccgcgcgt
     2281 gcagctgttg gtgtcttcgc aggaggcggc ggacagtggg ctcgaagggt tcgagttcta
     2341 tgactatccg ggcgcctttc tggaacgcgc ggacggcgag catcaagcga agctgagggt
     2401 gcaggagtgc caagcgttac gtgaggtggt ggaggcggag gcgaatgcgg cggctctgtt
     2461 ctctggcagt ttgttcgatc tgacggacca cccggatgac gagcagaatc gtcagtacct
     2521 ggtggtgggg aatcggtttc ggttgaccga gtacgagcgg gtctccggct cgcaggcttc
     2581 cggcgccgat cagcagacgt atcagagcaa tctgcttttg atccccgcaa atcatccctt
     2641 taggcccgag cggaaaacgc ccaaaccggt gatcgcgggg ccgcaaaccg cgatcgtggt
     2701 ggggccgagc ggctccgaga tttggacgga tgagctcgcg cgcgtgaagg tgaagtttca
     2761 ttggcatcgc gaagatccag cgaacgagac gagctcgtgt tggattcggg tggcgcagag
     2821 ctggtcgggc gcggggttcg gagcgcagtt cgtgcctcgc atggggcacg aggtggtggt
     2881 ggagttcttg gagggagatc cggatcgacc tttaatcacc gggtcgctat acaatgggat
     2941 cgacaatcct ccgttcgatg agcccacgca aagtggtgtg aagtcgcgca gcacgaagca
     3001 gggttggccc gcgaacgcca acgagatccg cttcgaggac ttgaagggca gtgaagagtt
     3061 ctacgtgcag gccgaaaagg atctcgaggt tctggtcaag aacaacgaaa ctcgtcaaac
     3121 gggggtggac cgctcgacgc ggattggagc caacgacgcg ctcacggtcg gggtcgatcg
     3181 cagcggaacg atcggtgcca atgatagctt gaaggtcgga gcgaactcgc agacggtgat
     3241 tggcggcgcg caaagcgtgg tggtggcgca agggcgcagt atcacggcct cggcggaggt
     3301 gctgaatgtt ggcacgcgca gcaagatcgt cggcaccacc gaatcgatcc gcgtcggagg
     3361 cgcccgcacc gaagccgtgg gcggggacgc aaagctttcg atcgctggca aggcagagtt
     3421 gtcgattggg aaggaccaca aggtcaccgt cagcggggac cgcaatgagt ccgtaggcaa
     3481 gaacgacagc ttgaaagtgg agaagaagct cgagatcgag gccggagacg agatcgtgat
     3541 tcggaccggt caggcgagca tgacactgaa gaagaacggc gacatcgtga tcaagggcaa
     3601 gaacctgaag ctagacttcg atagcaaagt gaacgtgaag gccggctcgg acatcgccct
     3661 gaagggcagt aagatcggtc agaactgaga cgaggagtcg atgccaaaac gagcttccgc
     3721 ttgccccttg aacgacgact catcgaacga tggcgccgaa gacctagagc cctcggtgga
     3781 tagcacgacc ttcaccgcga ccctgggtac cgtgtctgcc ttcccagagc tcgcgcgggt
     3841 tacgcgcatc gacccagagg gatatgtctg ggtcgcgctc gaagacggcg gggccgagcg
     3901 gcgcgcgaga ctcgcgctgt gcggcgaagg gctctcgacg gacatgacgg tggccgtgcg
     3961 cttcgaacgc ggtgaccggc gttgtccggt aattgtcggg ttggtgtctg accgcctacc
     4021 cccaggcgaa gacgtaacgg cacgcgagga acccgcaaag cccacgactc aaggccgcga
     4081 tctcacgatc accccgagct tggatgggcg aagggtacag gtgcgcgcag aggacgagat
     4141 tgtgctcgag tgcgggcagg cgtcgatcac gctgcgacgc aatggccgag tggtggtgcg
     4201 gggcacgcac gtggagactt attctgaagg tgtcaatcgc atcaagggcg cgcaggttcg
     4261 catcaattga ggatgacgat gtctgctccg cgccgcgtga gaccccctcc gttccgctgg
     4321 gatttcgtgg aggagagttt ttctgaagcg gagtttctgt ggggacgcag gcagtcggcg
     4381 ctggagagta cggagtacgt gctgtcggag ctgtcacgtt tcatcgaaga gcgactgttg
     4441 ggttgcctcg acggggtgat gctggcgggt gaggcggcac acgagcggct gctcgtcccc
     4501 gcgctgctgg gtgaagagcc gaggctcgcg agcgtggcgg cttggtgttt ggcgtcctcc
     4561 ggggaacgcg gattgtcatt ggtcgcagcg gtgctcgagc aagcggagga gccgagttta
     4621 caggctctgc gagccgggct cgaactcgtg atgtggccgg agttgggcgc gcggctcgag
     4681 gggctgcgga atgcagcgag cccaagggtg catgcggccc tgctcgaggt gtttgcgttt
     4741 caggggcgag gtgcgggcgc gacgttgaac gagctcgtgc gtcaccctga cccgatgttg
     4801 tccgcggcgg cgctgagggc cgcgcgtttc gatgcgcggc ctgcggctga gattgctgtg
     4861 gggcagggtc ttgaacacgc tgcggccgtg tgccagtggg ccgcaatcga gacgggcttg
     4921 gtgctagggc tcgaggcagt gcgcggtctg gcgttggagt cgtggaagca gccctggcgc
     4981 ccggagtacc atcgggtttg ggcgctgacg gcagctttga atcccaagca gagctcgctt
     5041 accgcggcgc tgggtgatga gaaggggctt cccggagcag cctgggctct aggtttcgcg
     5101 ggcactcgcg acgcggcgga ccactgtgtc gcgctgatga ccgagggcag ggtgccggaa
     5161 ttggctttgg attcgttctg caacatcgtc ggctgtgatc cggtcgtcga gcgcctggcg
     5221 caagcacctg cgccgcccgc ggattcgcct gaaactctgg atgaagacga cttggacgcc
     5281 gacttacgcc tcgccgtgga agtcgctcgc tgggtgccgg acccggcgca agtcaccgcg
     5341 tactgggcca ggaggcgaga acagttttca cccgagctac gttatcaacg cggccgcgcc
     5401 gtgagctcac cgagtgagct gcgcgagctc ctgtgggacc tgccgctcgg gcgtcggcac
     5461 gtgacggcgc tcgagttggc ggcgcgcacg tgcggagtcg agcagttcaa cccccgggct
     5521 tggtgccgtg agcagcgcgc tcggctgacc cagctgaccc aagccacgcc cgaaaccgcc
     5581 cctttgcccg agccggtctg agcatgttcg aggtggtcaa tcgcacgccg tttcccgcag
     5641 cgctcgtgcc gtttcgagat ctggcggggc gcgactacct ggggctcgtc atcaaggcga
     5701 cgttcgagct ggaggcggcg tacaagcgac cgtccgccgc ctccccgggg gagatccaga
     5761 ttgccgaaga gcagccaccg cctctgacga gcgatttgcg aaatggcaaa acgccgaccg
     5821 cgagcgcccg tgtcttggcg gagctcacgc gacccaagct cgctaccgat ttggtactcg
     5881 tgggtcacgc ctgtaccgag agcccccgag atcctcaggt ggatgtgtgt ttgcgcgtcg
     5941 gtcatcacgc caaattggtg cgggtgttcg gggagcgggt ttggtgccga gcggtgggcg
     6001 agtgggtgat cagtgacact ctgccgttcg agcgcatgcc gttggtttac gagcgggctt
     6061 atggcggggc ggataccagc gaccccgcgc ccgtgagaca tggatacgag ccgcgcaatc
     6121 cggccgggac gggcttcgtg gcgcacggct cttcggtgcg actggagggc ctacgtctac
     6181 cgaatctaga ggatccccga gacttgattg gttcgtggaa gcagcgtccg aatccggcgg
     6241 gttttggcta cgtggacgcg cagtctgaat ggcgctcgcg ctacgcgggc acctacgatg
     6301 acgcttggcg cacgcaacgc gccccactct tgccgctcga tttcgacgat cgcttcttca
     6361 acgcggcgca tccggagctg atttatcctg ggtatctcca gggcggcgag ctcgtgaggg
     6421 tggacggcgt ggcccctcgt caaacgttgt gtttcgaggt ccctcgcttg aagttctcgg
     6481 ccgcggcgtc gattcacgac gatacgctgg acgcagcgcc ccgactcgat accttgtgga
     6541 tcgacgccga cgcggcaaag ttggtgttgc tgtatcgggc cgcctttccg acgccgcgat
     6601 cgggcttcgg ggtgcgtgca gtgttcgtgc ggaggcacgc atgagcttcc gactcgtgat
     6661 ctcgggcgtc ggcgcggtga gttcggtcgg cgccggcgcg cttcagacct gtgcgtcaat
     6721 ccgcgcgggc ctctctggtt tacgcgaaca tcccgatttc tatccgcagc cgccgaatcc
     6781 tgtgatcgag ccgcccgagc gggtcagcgc ggggctcgtg ccgctcgggc tctcgttagc
     6841 agctcggcgg gagagaatgc tcgagcttgc cgcgcgtgct gcccacgaac tcatcacctt
     6901 ggccccgatg gcgcgcggtg agacggagcg tacggagctg gtgttgtgcg tggcgagcgc
     6961 ggctcagggc gggttcgacg cagcggaggc ttcggagctt acgtcggcgc tggggcaacg
     7021 cctcggtctg cagctcgagc ggcccttacg caccatatcg caaggtgcga cgggcgtgtt
     7081 gcgagccgtc ggagaggcgc gcacccgtat cgcggctggc gaagtcgcac gggtgatcgt
     7141 agtcggcgtc gagtcctggg tgacgctgga cgctcttgcg gctctggacg aggcgcgacg
     7201 cttgcgtagc gctcggatcc tggacggcct catcgcggcc gaagcggctg cctgtgtgtt
     7261 gctcgaaaat gcggagaacg cccaggcgcg cggccgcacc gcgttggcgg agttcgcgag
     7321 cgtgggcttt gggcgcgagc ccaatacgct ggagggcgaa ctgaactcga gtggcgtggg
     7381 tctcacggag gcggtgcgcg cggccacccg cagttttggc cgcagcaatt ggccctggtt
     7441 gctgtccgat atgaccgggg agagttatgg cgcctatgaa ctcggactct tgcgggcgcg
     7501 gctcggtgag cagctcggct ccaccgaggt gtggcacccc gccgattgca ccggcgcagc
     7561 cggcacgggg tcggccgcgt tgggcctggt gatcgccgcg cgcgccttgg cggacggtta
     7621 cgcacccgct gatcgggtgt tgctttgcgt tggggcgcag ggcgaagagc gcgccgccgg
     7681 cacactcctg gcgtgcacgc aaccaggacg aagggagtag ttatgccatc taccgtagtc
     7741 gtcaactttc gtacggtcgt acatcagggc agtggcggtg tcgccaatgc gttcccggat
     7801 gtttgcaaga ctccaactcc cgcggggccg gtgcctatcc cttaccccaa tatcgctatg
     7861 tcgtcggaca cggctcaagg cgccaaaagc gtgaatgccg acggcaatcc gatcgccatt
     7921 cagggcagcg tgttctctca gagcaccgga gatgaagcgg gttcggcggg cggcgtggtc
     7981 tcgagttgca tccgcggcaa agccgaattc gtgatgtact ccttcgacgt caaagccgac
     8041 ggcaagggcg tggcgcggct cggggatctc atgttgcaga acaagggcag cgccccgaac
     8101 acgcctccat tcccagaagt tcaagctccc ttgccggtac tgccccccga tccggccgag
     8161 ctccaagaca acgacatcgt cgagatcgaa gtggtgactc cagaagagta cgaacagtga
     8221 atcaggaaag gtttgtatgc cgacccgact tcagtgggtg aactacccgg acgcggacaa
     8281 aaccatcggt gcaattcacg tgcgtcgcgc caatttcatc gacagtgacg tcggcactac
     8341 tggcgtgatg agcaccaccc tattcgtgcg cattcgcttc cagcgcagtg atgccgcagg
     8401 cgccgcgacg ctcacgattc gacccgacgg cggcaacgcc gcgtactctg cgcaggaacg
     8461 gctggggcag cccggcgtgt tcgcgccggg gttgacggcc gccacggtcg tgagcgcgca
     8521 aggctacgca cgctttcgag tcaaattgac ccctgccgga ggggacaagt accgcctgga
     8581 ggggcgctcg gccgcgggca ccgtgcgcaa ctacgcgaac gaagtcgaaa cccgccgtaa
     8641 gctgttctac cagatcatcc gcatgcgcga ggcacgcggg ttggccgctg gtatgaccgg
     8701 gcgggtcgag cggcagtttt ggcgacccga caagaaactc tacatcaagc tcgcgcagta
     8761 cgcgcgcggc cacaccattc gaaacatgga gaacttcgac gacaccgatc ccgccgtcga
     8821 agcgagagtg aagacccgcg cccgtcgcgc gtacgaccgc agcaaagctc cttttgcctt
     8881 cgttgccatc ttcgtcaaca agaactgcat tcccgctcga gagcatcagg tcgtagccgc
     8941 caacttcacg ggcgccccct tcgtgttgaa cactcaacgt cgcattttct ctcacgcgga
     9001 ccgcgcggtg cgctggtttc actcgatgac gttcactccc gcgggcggcg ctccggtcaa
     9061 cattccccgt cgcgcagtgc gcgtcaccgg gctccgtgcg ttgaccatca atacatctag
     9121 tttgcctgcc ggcgcgggaa cgctcgaata cgagattacc atcgtggata tcgagggcat
     9181 gggcctcagc ttgcccacgg agaacctcgt caccattgcc agccgatttt tggacggcag
     9241 ccccgtgccc gcggctacga tggcggccgt gatggtgcac gagattgggc acaagattgg
     9301 catggtccct gggccacaag gcgatcacga tctcgatcgt cagtccacct actacgatgg
     9361 acggggccac tccgggccgc actgccgtca ccctgctcca ctcgttccga cctataactc
     9421 cgctgctccg ctcgtgcccg cgccaacctg caccatgttc ggcgacatcc gcaccaacac
     9481 gacccaattc tgcacgaact gcgtgcgctc ggtgcgcaaa ctcgacttgg atcccgatcg
     9541 aaagcccggc attgcgacac agttttgact tcctgcctgg cgcgccgagc agcggcctag
     9601 cccgcttctt ccgggcgagc gtggccctac tgggctggtt gctgctctca gcctgcacca
     9661 agcccgcgcc tactccgccc gccggtcccg tatccacttc cccgagcggc tcagcggagc
     9721 ctactgcatc tccccgtcca tccgcgactg cgcccaatac gaggtcaccc gtgacagccg
     9781 acttctccaa ggtggtttat ggctatcagc cctccgagtg cactccgcgc ttcacgcctg
     9841 cagagccaga cggaatcaag atcgccatgc cgtcgcaaat ccaactcagc accgccgcca
     9901 cggtacgcgg caagccggcc ctgccactct gcatctcgat gcgcttcagc gccctctacc
     9961 tcgaacaatt cgatcccgtg ttcagattca tccaggtggt ggtgatcgac gacgcgggca
    10021 aacaagcctt tgccggcggt atcttccacg accggctcta ccgccccagc ccgccttcgg
    10081 acattccgga agccgagctc gccaagcgca ttgcttacaa gtacggcacc gtcaacctgc
    10141 tcgaattctt cgcgttaccc gagatcgaag ccacctatca tattcacgtt ctcttgaagg
    10201 aacaccgctc caacgtcatc accgttcaag tcgagccatg acccaagatc gctcgcgtcc
    10261 cggtcattct ccgcccgccg ccccggcaga gctcccgtgt ttcggtgcgt gtaccgagca
    10321 tccgaaactc gatgtcggaa tcgtgggatt gcgcatcgcc accgcgggag tatccggaac
    10381 ccgcgaacaa ccccgttgga tcctgcacgg cgtcctgcgt gttgcgcgag aactggcggg
    10441 cccgaacccc gaagagctct tcgcccgcat cgtcatcgtg cttcgtggcg ccagctccga
    10501 gaggatccag gcgcgtcacg ccttcc
//
LOCUS       DASTKU010000843        16059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_165154, whole genome shotgun sequence.
ACCESSION   DASTKU010000843 DASTKU010000000
VERSION     DASTKU010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16059
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165154"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..618)
                     /locus_tag="VFQ61_29520"
     CDS             complement(100..618)
                     /locus_tag="VFQ61_29520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET9958683.1"
                     /translation="MQSSVNTEAARHSFKVGEMAVHPNHGVGEVVQVEERDLGGRKTS
                     CYVIKIRDSGLKVMVPTEAASRVGLRPVMKKKEALKILDILKAPEVAVDLQPWNRRFR
                     AYTEMLKSGLPSEIAKVLRDMYRLKFDKDLSFGERRLLDQARSLLIQELALAKKVEPA
                     AMEGEIQQIFSA"
     gene            complement(1364..2359)
                     /locus_tag="VFQ61_29525"
     CDS             complement(1364..2359)
                     /locus_tag="VFQ61_29525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958684.1"
                     /translation="MAMIGSLLLGGAASCTRAPAAPAAPQKPLKPLHEGPLTDYVPAA
                     ALRWMLIGKPRSLLANPAIAPATKLLIPQTRWEAFELASGVDLRQVRDALVAGFDFGT
                     LYAFQPVAGTAPQAVKRFRDRIVSRERIYRPDPRIDRITGIIGTTPETLVRIDQSLVA
                     VAVGDPMPARVVEAFALGRLSRSPNALRGAALSTLGEPPKGALATFYAPGPFPQEWAR
                     GASGLLAQAFAARISMAPLDAERAHFELELTGEFPPDGAERLRVAYAALVQSALGKLV
                     GLDQPAAEPLVLERGDHLQLEVDLEIMPIARGLRAAVISDVWEILNLSAPNRAKD"
     gene            complement(2457..2774)
                     /locus_tag="VFQ61_29530"
     CDS             complement(2457..2774)
                     /locus_tag="VFQ61_29530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9958685.1"
                     /translation="MTKSELIEAIAARGELTKARAEMVVNCVFETMTEALKRGEGIEI
                     RGYGSFTVRPYKPYAGRNPRTGQPVPVPAKRLPFFKVGKELKELVNDSRHQPITGGGD
                     EDD"
     gene            complement(3011..5182)
                     /locus_tag="VFQ61_29535"
     CDS             complement(3011..5182)
                     /locus_tag="VFQ61_29535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958686.1"
                     /translation="MASSQNPYLEFGLAEDPAAWVALGVAVLAALGLSFRSKPCSLAR
                     PRRGLALLALMAGMLSHGYVHHYLRGGPRIIDATHYLLEARTIAGGEFAFTAPSPASA
                     VRGRFLVSVPNRPERVGVLFPPGYPALLALGVLLGKPMWVGPLLAMLLVLATYAVARS
                     WQFTEQEALVASGLSVISGTLRYHTADTMSHAWAALLVLLAAWSHAPRTTRGPPRLSE
                     PPLAARYFAFTSRREFWSGLEGLSAGLLVATRPVTGAALTLVLLARRVSSTRHSFSAG
                     ARGLAVWFTAASLGVAPLLLQQHALTGSFWGSVQLAYYAAADGPPGCFRYGFGSGIGC
                     LFEHGDYVRARLPQGYGLLAALATTARRCLTHSLDLANAAPIALALPVAMYRGRREPS
                     TLFFSSVVVAVVAAYVPFYFEGSYPGGGARFYVDALPIEHVLLARGLVGLASTFGRST
                     WISWVWPVTLLGFALHTSAHHRSLRDREGGRPMFEPEVLSQARVSKGLVFVGTDHGFA
                     LGHDPRVQAASRGLVVARERRDASDSWLWHALGKPPVYRYVYEPSARVATPALVPLAT
                     AELDSRRATRRFEAESSWPPLAVQGGFAYPTYAPHPCVSRGRGLLVRATGGVLGVRLL
                     LPRPPERAERDSARWRLGIWIPANDSPARTSADHLEPRPIVRALSSVGSAQHWERELD
                     LQPIHGECWQARLPIGRPAAWGSEVELRLSGSVFLDFFELE"
     gene            complement(5182..7371)
                     /locus_tag="VFQ61_29540"
     CDS             complement(5182..7371)
                     /locus_tag="VFQ61_29540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958687.1"
                     /translation="MGDTPAPEPRDPRNSKKRFQQALYALLAAFVTLAVATRFYGSLR
                     LQTLYSLHWANPDQFDLLASRVKLGPWSAPLDDVFIHFDFARSIARGYPFQWSEGNGY
                     SSGGTSLLYPFVLAVGYLAGFRDLELMEWAAVCACICCFAALLAARRLARGLPSAAAL
                     LLPLSLYSVGALSWSLFSGMEVALFVALWALAYSVYDAIESAAWSATWRSALCLGGAG
                     LAVTATRPEGIVMVAVLALAAAWRVRHSGRLQGLAVAAIAIAPSLLLLGFHGLMNRVF
                     TGESRAAGALVKLELNHPYLTAQQVFEAWVFHVKYQVLRVTHYHFADVPVFGYIVWLF
                     GAAALLFQRTRQQALILWGSAAAWVLFVALNGQVRWQNERYTMPAVALLLFAAALGVG
                     GLATWASERRQLWKSAAALTGVVVLLGLFWWHQAPRYREQLWFFGRASRNIYDQHVQV
                     GRALRNEFPQPPKRVLVGDAGAIPYISDLPALDIIGLGGYGKLPFARATRQHVGAAIE
                     LIEHIPPAARPDLLAIYPGWWGDFPVWFGERIGEVPVRGNVICGGASKVLYRPNWSPL
                     ERSGEPFAIAPGQRLLDVLDVADLLSEREHRQELTGAPGFVAMKLLDSPATRRPLWDA
                     GRVLPPGARLDFELNAGGRGPRAERLRIVVRLTHSEGVHLALDLDGRRVGVLPAQIHD
                     GWVEVASEPISLRSIEANVSSGGKHRITLSSNDHECVVYHVFVIAEP"
     gene            7742..10258
                     /locus_tag="VFQ61_29545"
     CDS             7742..10258
                     /locus_tag="VFQ61_29545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958688.1"
                     /translation="MRWFVEVSRVGEGAPAERYCVEAKAWQAALQETRKMRGDSGPLS
                     KFSIELLDDGYRAVDPVQKIRYLVNQAPADAALNPPTPPLNGTSQHAAAAPSTAPDLF
                     LGSNQKSSVVLPSSAGPAPNAGALFNAGVSPGPVSVPGVASNSTATPAPEQGKSPPLD
                     AKSTEESMGRTKNSSLAQSSPGAAATPAHPSPAEEVAKVAATAGLLGSGGTPSVSALR
                     AAEPVEPAEPAKPAEIAKLAEPTKVVESIAPRPAATKPQPKPLSLRPRESTSPRPVTV
                     PPRASVPAPRESRESVSPAVVVTPSVVVAAPRIEPAVVKSAVVSVPPPRPSDAVVTPS
                     AALKVLLFRSQDPRPDAPITYREAAYYVPPGTSRAEVERLLLDRLAHFKEELRGVTAG
                     RLVQIALFDHEFDQRPAAPPLGTLAWKDWRGEPVLSFPAFSERSSVAPPSPEARAHTA
                     VEHSSVTSAPSTASSSVAPTSEAHVSGQGSASSVSTGAAPAGAASPTAADSSAPAPAE
                     PVGTGSVEEAAASDALTLNNVASSSGSAVATTGSVDRATSGGEAGEKIATENPTRETP
                     AETDALAEKPRGHDATEHEVSALAPPSPAAAPEIAPPNAASESNQSLAPVPSEAPPAT
                     DARANESARPSSPVELRDSKPSLGSVERPSRGRMRVGRRRAGEDLISELFEVMHELHF
                     AHDLMTGTDFLLGTLLDLIPCEVTMLEVFDINTRQFVVVRGQGGGASSTVLQRTSDTD
                     PLVGEIMRRGASRVFRATNDARFHTGRFGKLTAHLEQVLCGPVRQGGRYLGLIELGNP
                     LGGAPFHEGEVNALDYICQQYAEFVASRPVVLDSDVILGR"
     gene            10258..11853
                     /locus_tag="VFQ61_29550"
     CDS             10258..11853
                     /locus_tag="VFQ61_29550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9958689.1"
                     /translation="MADRPRRERWLTFLLLPAIVVAVLVLVWVMFRSSFQLEKLREQS
                     VIEATLLLANEKADRLDKRIIEQDNVIRSTLAVDEREDFGARWREAAQLQTPTVRAVL
                     VLDLSAPDKPVVAMASRAPGPESERFRRLLVHAMLPDLKLERPPLEELRHLHKTYRGQ
                     NYLLSHWETESAGRRYLTVVWHDVSRIVHDVFPELYADAQQSRVNVVDAEGRIVFGPA
                     QSRGGLTLGRQFETTLYKWTLNVTMNSAEELATAVARRRLLEMALVGLSGVVVILGII
                     VIVVAAERERKLSNLKGDFVANVSHELKTPLSLVRMFGELLQSGRVDSEDKRKQYLQI
                     IVSESERLGSLIENVLDFAKVERGKAAYEFVEANLTDVVTRAVEACRPRAEREQVEIE
                     TEFPDGPQLAWIDERAIEIAVINLVDNALKYAPGGKRVQVAVRRRRDHLEIRVSDQGP
                     GIAPEDRKRIFERFVRGQSAHGKQVRGSGIGLALVKHIAQAHGGACDIEDGTPVGATF
                     VFTLSMAPRARHRLLPFSSRATS"
     gene            complement(11932..12477)
                     /locus_tag="VFQ61_29555"
     CDS             complement(11932..12477)
                     /locus_tag="VFQ61_29555"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9958690.1"
                     /translation="MGAYRAKDLLLWPNLVSFTRLPLAYAFVEHVRETSTALVILAVS
                     GFTDVLDGFLARRLGMATPTGAVVDGVTDKIFALTVLVTLIARAQLSPLSALLLGTRE
                     FGELPLVVWWALHRERRRAKAEDPRANWLGKAVTVLQFACVALVLSSNRFALVLIATT
                     AVLGVGAAVSYWRRELADSRG"
     gene            complement(12491..14638)
                     /locus_tag="VFQ61_29560"
     CDS             complement(12491..14638)
                     /locus_tag="VFQ61_29560"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET9958691.1"
                     /translation="MIDPSVREDGETDPAFESELGAPNPAPKSESSDSTPQREQPAAQ
                     GGAAELGGGERRAPEPESNRTEPNRAEPSRLEPEHVEPEWDAAAGQSELPVEELVSSG
                     DAGRSVERVQSGLPRGGRAREVLLERLEATRRAQGEAALGDLDEIGDFDEEVVERPAP
                     QPVAEPPGVSPFAPLPFSATSPPPRRSRLSPNWIALIGTAVGVATVSSLVALGMNVDL
                     KVPVTLPTSTSAPPPQAPSAQEPAPPPAPKRERAKEKGPPRVGDMRSDASVRIVEGKI
                     GTDAFLKALEKAGVEMRENYRIVASMKGVRDFDKCKKSDRFVVAIDRASSRVKGFEYV
                     VTPEEVYQSLEGEGGRLKGSKLDLKVAHNPIRGSFVHDGKSFDASAQRGGFEAGLARA
                     VAKALDGHMTMDEISRGARLRVIAQEVTVLGEFARYAGVEALEIQDGPDEKPFRLYYF
                     DNPKERGYYDAEGHAPYEGGFRKPIKDAPMTSPFNLKRMHPILKKVMPHLGIDFGSPM
                     GTPVGASSSGVVSHASYIGPTGNLVKIEHGGGIETGYAHLSRFAEGLKVGDKVKRLQI
                     IGYVGSTGRSTGPHLHFSARKNGEFIDPMTLNMDALRTLSRESRAAFAELKAKYDAQL
                     DALPLPASLPAEAVASKGAEPKPEKPTEPAVAAGPTPSAAAATAGEVDSGDEEDAAPS
                     PQAPAKKPGSSVYLTDKELLELQGASDEGEVAE"
     gene            14843..>16059
                     /locus_tag="VFQ61_29565"
     CDS             14843..>16059
                     /locus_tag="VFQ61_29565"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HET9958692.1"
                     /translation="MRQPSPPSATPRTGFANRVKLARWPRLRSLIQLLRGRGSRKQSA
                     WSVGVGLFIGCLPLYGLHFPLCVAVCVPFGLDALIAYLAANVSNPLLLPLILLAEVEI
                     GSWLLHGQSAEFTLDAARRLGALGFGTEVALGSVVFGAALALLGALLTARFVRDRPPT
                     PLELALRRTQALYTGAPAADRHYVRFKMALDPVIAQVAELAARHGPAGWGRVLDAGCG
                     RGQLGLLLLELGAAADLTGFDFDTRKVEVARGALAHASANGGRARFYVGDIAQAELAG
                     FDTILLIDVLHYLSRDEQDRLLTRVARALPVGGRLLLRETDSRRSASSLLSKSFEYLA
                     TRVGYNRSAHRLAFRSLPELTRYIENLGLKCAQIGAEHGVAEQAGEHGVAEGRFTEGQ
                     GTLSNELLLVEKTR"
BASE COUNT         2951 a         5347 c         5050 g         2711 t
ORIGIN      
        1 ccctctcata acagaaacgt ccggagcaaa gcccggacgt ttctagaccc gtttcaagac
       61 cacggggatc tcgcgcccta agacgcgcga ttccgcagtt caggcagaaa agatctgctg
      121 aatctcaccc tccatcgccg ccggctccac tttcttggct agcgcgagct cctggatcag
      181 aaggctccga gcttggtcga gtaggcggcg ctctccgaag gacaaatcct tatcgaactt
      241 gagccgatac atgtctcgca aaaccttggc gatttcggag ggcagaccgc tcttgagcat
      301 ctccgtgtaa gcacggaacc tgcggttcca aggttggaga tctactgcga cttcgggcgc
      361 tttcaggata tcgaggatct tgagcgcctc cttcttcttc atgaccggac gcagcccaac
      421 gcgcgaagcc gcctcggtgg gcaccatcac cttcaggccg gaatcccgaa tcttgatcac
      481 gtagcaagaa gttttgcgac ctcccaggtc ccgctcctcg acctgaacca cttcgccgac
      541 tccatggttg ggatggaccg ccatctcacc aaccttgaag gagtgtcgag ccgcttccgt
      601 gtttaccgac gattgcattc cgcctcctaa gatcccgacg agttccgccc gcgaaagtgc
      661 aggtcgtcag gtgtattcgt tttccgcgcc gaactgtgtg ctgcgcggtt ccgcggcgtc
      721 gcagggccag gccgacctcg acctggctac ccccgcgtgc gaaacccggg ccctacgtaa
      781 aactccgacc gccagacgcg agaccgacgc tgatacacgt aaggggacag cggtcccgcg
      841 ccaaacatcg ggctggtgac cgcgccgctt cgattgccgt ctatccgacg aacaccgccg
      901 cgccgccgat ggacttccgc accgcgcccg atcagtgcgc gctgcgactt cgaggcactg
      961 tttagccgcc cagcacagct cggaagccac ccagcgcagc tcggaaggca cctggtctcc
     1021 ggccgccgta agatccgcta aagcagactc tgcctcagca cacactaggc gcctgtcgcg
     1081 tgggttgccg cgtcgcttca ccgttcgatt cattggatca cgccgcgccg cagcacgcag
     1141 gggtcttatc attcgaaggg gtatttggtc aattagaacg cgtctccgaa ctacgaagta
     1201 gcgactcgaa atcgtttcgg aattcacccc aacgggtaag ccgttggcgg gccatgtgtg
     1261 acaaaccatc acctattccg cggctttttg gggatgagtc caaatcgata tcgaactcac
     1321 cacgcgaatt cggcggcttc cgcgggctgc actcgagacg acgttaatct ttcgcgcgat
     1381 ttggcgcgga taggttgagg atctcccaaa catccgaaat cacggctgcc cgcaagccgc
     1441 gagcgattgg catgatttcc agatctactt cgagttgcag gtggtccccg cgctccagca
     1501 ccaacggctc tgccgcgggt tgatcgagcc caaccagctt gcccaacgcg ctttgcacca
     1561 gcgcggcgta ggcgactcgc aagcgctccg cgccgtcggg agggaattcg ccagtcaatt
     1621 cgagctcgaa atgggctcgc tcggcgtcca acggagccat tgatatccgc gcggcgaagg
     1681 cttgcgccaa tagacccgat gcaccgcgcg cccattcctg gggaaaggga cccggcgcgt
     1741 aaaacgtggc cagcgcgcct ttgggaggct caccgagcgt cgaaagcgcg gcgcctcgca
     1801 gcgcattcgg agagcgtgat agccggccca aggcgaacgc ttccacgacg cgagccggca
     1861 tgggatcgcc gacggccacg gccaccaggc tttgatcgat ccgcaccaga gtctctgggg
     1921 tagttccgat aatccccgta atccggtcga tgcgcggatc tggccggtaa atgcgctcac
     1981 gagatacaat ccgatctcga aagcgcttta cggcctgggg tgccgttccg gctaccggtt
     2041 gaaacgcgta caaagtgccg aagtcgaagc ccgcgaccaa ggcatcacgt acctgtcgca
     2101 aatcgacgcc gctcgcgagc tcgaacgcct cccagcgcgt ctgaggaatc agcaacttgg
     2161 tcgcaggggc gattgccggg tttgccaaga ggctgcgcgg tttaccaatc agcatccaac
     2221 gcaatgcagc tgcgggcacg taatcggtga gggggccctc gtgcagcggc ttgaggggtt
     2281 tctgtggcgc tgcgggagcg gcaggtgcac gggtgcagct agctgcaccg ccgagtagca
     2341 ggctgccgat cattgccatg gcgaaccgca gcccgacacg tttgctccgc cgcggtgaat
     2401 tccgtagcga tcggcgcgcc cccttcaata cctgcatttt ccgctggatg gatggctcaa
     2461 tcgtcttcgt caccaccgcc ggtgatgggc tggtgacggc tatcgttcac cagctctttc
     2521 agctccttac cgactttgaa gaacggcaag cgcttggccg gtacgggcac cggttgcccg
     2581 gtgcgaggat tgcgccccgc gtaaggtttg tagggacgca ccgtgaagct gccataacca
     2641 cggatttcga tgccttcacc ccgtttcagg gcttcggtca tggtttcgaa gacgcaattg
     2701 accaccattt cggctcgggc tttggtcagc tcgccacgtg ccgcgatggc ctcgatgagc
     2761 tcgctcttgg tcatcgctct gccggcctcc ctctcgacgg gttcaccccg ctcctcgtgg
     2821 ctgcaccggc ggggtgaacg cttgaacgag gacgcctcca aacgcgcccc ccagcactgg
     2881 cttgaaaccg agatgcagca tcgttcagtt gagaactgtc agacgtaaga gctgaattgt
     2941 caacgccttt tagccgagca ccgcgggccc actcgtgtcg ccagggccgg caggggtgtg
     3001 tcccgatcgc tcattcgagc tcgaaaaagt cgagaaagac cgagcccgaa agccgaagct
     3061 cgacttcact cccccaagct gccgggcgac caatcggaag cctggcctgc caacattccc
     3121 cgtggatggg ctgcaggtcc agctccctct cccaatgctg tgcgctgccg accgaggaga
     3181 gggcccgaac gatcggccta ggctccagat ggtccgctga cgtccgggcc ggagagtcgt
     3241 tggcgggaat ccagatgccc agtctccaac gagccgagtc tcgttccgca cgctcgggcg
     3301 gtcgcgggag aagaaggcgc actccgagca ccccacccgt cgcgcgaacg agcagcccac
     3361 ggccgcgcga gacgcaaggg tggggtgcgt aggttggata ggcgaagccc ccttgcacgg
     3421 ctaacggggg ccaggagctc tcggcttcga agcgccgggt tgctcgcctt gaatcgagct
     3481 ccgccgttgc gagcggcacc agcgctggcg tcgcgactcg cgccgaaggt tcgtacacgt
     3541 agcgatagac agggggtttt ccgagcgcgt gccataacca cgaatcgctc gcgtcccggc
     3601 gttcacgagc aaccaccagg ccccgcgacg ccgcttgcac tcgtgggtcg tgcccgagcg
     3661 cgaatccgtg gtcggtgcca acgaacacca aacctttgga aactcgcgcc tggctcaaga
     3721 cctctggctc gaacatgggc ctgcccccct cgcgatcgcg cagcgagcgg tggtgcgcgc
     3781 tggtgtggag tgcaaaaccc aacaaggtca cgggccagac ccacgagatc caagttgatc
     3841 taccaaaggt cgaggccagc ccaacgaggc ctcgcgcaag gagcacgtgc tcgattggca
     3901 gcgcgtccac gtagaaacgt gcgccgccac ccgggtagct tccctcgaaa tagaaaggga
     3961 cgtacgccgc gacaacggca accacgacgc tcgagaaaaa gagtgtcgag ggctctctgc
     4021 gtccgcgata catcgcgacg ggcaaggcca aggcgatcgg ggccgcgttc gcgagatcga
     4081 ggctgtgcgt cagacagcgg cgggcggtgg tcgccagggc cgcgagtaag ccgtagccct
     4141 ggggcaaacg cgcccgcaca tagtctccgt gctcgaacaa gcagccgatc cccgagccaa
     4201 atccgtaccg gaagcagccc ggggggccat ctgcagccgc atagtacgcg agctgcacgg
     4261 agccccaaaa gctgcccgtg agcgcgtgct gttggagcag caaaggcgcg accccgagcg
     4321 aagccgcggt gaaccaaacc gctagaccgc gtgccccggc ggaaaacgag tgacgcgtgc
     4381 tcgagactcg acgcgccaag agaaccagcg tgagcgccgc tccggtaacc gggcgcgtcg
     4441 ccacgagtag cccggcgctg agcccctcca gcccactcca aaactcccgg cgtgaagtga
     4501 acgcgaagta tcgagccgcg agcggcggct cgcttaaacg cggtggtccg cgcgttgttc
     4561 gaggggcgtg gctccaggcc gcaaggagca cgagcagcgc ggcccaggcg tgcgacatag
     4621 tgtctgccgt gtgatagcgg agggtgccgc tgatcacgga caagccgctc gcaaccagcg
     4681 cttcctgttc ggtgaactgc cacgagcgcg cgaccgcata ggttgcgagc acgagcagca
     4741 tcgccaacaa agggccaacc cacatgggtt tgccgagcaa cacgccgagg gccaacagcg
     4801 ccggatatcc gggcgggaac aaaaccccta cccgctcggg ccggttcgga acagaaacca
     4861 agaatcgccc tcgcaccgcg ctcgctggcg agggcgccgt gaacgcgaac tcaccgcccg
     4921 caatggtgcg cgcttcgagc agatagtgag tcgcatcgat gatgcgcggg ccgcccctca
     4981 gataatgatg cacgtagccg tgcgacaaca tgcccgccat cagcgccaac agcgcgagcc
     5041 cgcgacgtgg tcgagccagc gagcacggct tcgacctgaa gctcagaccc agcgcagcca
     5101 gcacggcgac ccccaaggcc acccaagccg cgggatcttc ggcgagtccg aactcgagat
     5161 agggattttg cgagctcgcc atcacggctc ggcgatcacg aatacgtgat acaccacgca
     5221 ctcgtgatcg ttcgaggaca acgtgattcg gtgcttgccc ccgctcgaga cgttggcctc
     5281 gatggagcgc agcgagatgg gttcactcgc aacctcgacc cagccgtcgt ggatctgagc
     5341 gggcaaaacc ccgacccgac gtccgtctag atcgagggcc aggtgcacgc cctcgctatg
     5401 cgtgaggcgc actacgatgc gcagccgctc ggcgcggggc ccccgcccgc ccgcgttcaa
     5461 ctcgaaatcc aggcgtgctc ccggtgggag cacgcggccc gcatcccaca gcggtcgacg
     5521 agtcgctgga gaatcgagca gcttcatggc gacgaagcca ggagcccccg tcagctcttg
     5581 ccggtgctct cgttcactca gtaggtcggc gacatcgagc acgtctagca ggcgctggcc
     5641 cggcgcgatg gcgaacggct ctccgctgcg ctcgagggga gaccaattgg ggcggtagag
     5701 caccttgctc gccccgccac agattacgtt tccgcgcacc gggacttcac cgatacgttc
     5761 accgaaccag accgggaaat caccccacca acccggataa atcgcgagca gatcaggccg
     5821 cgccgcgggg gggatgtgct cgatgagctc aatcgccgcg cccacgtgtt gtcgggtagc
     5881 ccgggcgaac ggcagcttgc cgtagccacc gagcccaatg atgtctagag cgggtagatc
     5941 gctgatgtag gggatggcgc ctgcgtcacc gacgagcacc cgcttgggcg gctgaggaaa
     6001 ctcgttgcgc agcgctcggc ccacctgcac gtgctgatcg tagatgttgc gtgaggcgcg
     6061 cccgaagaac cagagttgtt cgcggtaacg cggcgcctga tgccaccaaa acaatccgag
     6121 cagtacgacc acgcccgtca gcgctgccgc gctcttccag agctgccgcc gctccgaggc
     6181 ccacgtggcg agcccgccaa cgccgagcgc cgccgcaaaa agcaagagcg cgaccgcggg
     6241 catcgtgtag cgctcgttct gccaacgcac ctgaccgttg agcgccacga agagcaccca
     6301 agccgccgcg ctaccccata gaatcaacgc ctgctggcgg gtgcgttgga acagcagcgc
     6361 cgccgcgcca aacaaccaca cgatgtaacc gaacaccgga acatcggcga agtgatagtg
     6421 agtcaccctc aacacttgat atttgacgtg gaaaacccag gcttcgaaca cctgttgggc
     6481 cgtcaaatac ggatggttga gctccaactt cacgagtgct cccgcggccc gactctcgcc
     6541 cgtgaacacg cgattcatca gcccgtggaa tccgagcaag agcagcgaag gtgcgatcgc
     6601 gatggcggct acggcgaggc cctgcagccg cccggagtga cgcacgcgcc aagccgccgc
     6661 gagcgccagc acggccacca tgactatgcc ctctggtcgc gtggccgtga ccgcgagtcc
     6721 tgcgccgccc aagcacagcg ccgatcgcca agtcgcggac caagctgccg attcgattgc
     6781 gtcgtagacc gagtaggcga gcgcccacaa cgcgacgaat agagccactt ccatgccgct
     6841 gaacagggac cacgacaacg cgcctaccga atatagcgag agcggcagta acagagccgc
     6901 cgcgctcggg agcccgcgcg ccaaacgtcg agctgccagc aaagccgcga aacagcagat
     6961 gcaggcgcaa accgccgccc actccatgag ctccaaatct cggaaacccg cgaggtagcc
     7021 gacggccaac acgaacggat agagcaagct cgtgccacca ctcgagtagc cgttgccttc
     7081 gctccactga aacggataac cgcgcgcgat ggaccttgcg aaatcgaagt ggatgaagac
     7141 gtcgtcgagt ggcgcagacc aggggccaag ctttacgcgc gacgccagaa ggtcgaattg
     7201 gtccgggttc gcccaatgca gcgagtaaag ggtctgcagc cgcagcgagc cgtagaaacg
     7261 ggtcgcaacc gcgagagtga cgaacgcggc gagcaacgca taaagcgcct gctggaaacg
     7321 tttcttactg ttgcgcgggt cgcgtggctc aggtgcgggg gtgtccccca tgggacgccg
     7381 catttaccat cgaaagggtc atctgggctg gatttcctgc ccgacgtcga cccttctcgc
     7441 ggagaaagac gccgtcggcg ttagtcattt gcctgcccga acgtccggta gtgcaccttt
     7501 cttccacgac ctgacactag atgtggtgct gcgacacgtg atcagggcga agtcatgatt
     7561 agaagacgct gcaaccaccc atatatgggt atgcctcgcg cccttgcccg aattagtaat
     7621 cggcgctcta ctagcccagg ctcaaaacga gagcccgccc agagagccgt gaaccgacga
     7681 aattcgctac gccccattgc cgccacccag gcgctatcgg ttacgactat acggacacga
     7741 gatgcgctgg ttcgttgaag tctctcgagt tggcgaaggt gcgccagcgg aacgctactg
     7801 tgtggaagcg aaagcctggc aggcggcgct gcaggagacg cgcaagatgc gcggtgactc
     7861 cgggccgctc tcgaaattct ccatcgagct actcgatgat ggctatcgcg ccgtcgatcc
     7921 ggtgcaaaag attcggtact tggtgaatca agcaccggcc gacgcggcgc tcaatccgcc
     7981 gacgccgccg ctcaacggca cgagccagca cgcagcggcc gcgcccagca cagcgcccga
     8041 tctttttctt ggttcgaatc agaaatctag cgtcgtgctg ccatcgagtg cgggcccggc
     8101 gccgaatgct ggggcgcttt tcaacgctgg ggtttcgcct ggccccgtct cggtacccgg
     8161 cgttgcttcg aactccaccg ctacacccgc acctgaacag ggcaagagcc cgccactcga
     8221 cgccaaaagc accgaggaat ccatggggcg aaccaagaac tcttcattgg ctcagagctc
     8281 accaggcgcg gcggccactc ccgctcaccc gagccccgca gaagaagtgg ctaaagtcgc
     8341 cgccactgca gggctcctcg gatcgggcgg aacaccgtcc gtgtccgcct tgagggctgc
     8401 agagccagtt gaacctgcag agccagccaa gcccgccgag atagccaagc tcgcagagcc
     8461 caccaaggtg gtcgagtcga tcgcgcccag gcctgcggcg acgaaacctc agccaaagcc
     8521 actttcgttg aggccacgtg aatcgacgtc gccgcgaccg gtgaccgtgc cacctcgggc
     8581 cagcgtaccg gcgccgaggg agagccgtga gtccgtctca cccgccgtcg tagtgacacc
     8641 ttccgtcgtc gtcgccgcgc cgcgcatcga gccggcggtc gtcaaatcgg ccgtcgtgag
     8701 cgttcctccg ccgcgcccat cggatgcggt cgtgacccct tcggccgcgt tgaaggtgct
     8761 gctgttccga tcgcaagacc cgcggcctga cgcgccaatt acgtatcggg aggctgcgta
     8821 ctacgtgccc cccggcacga gccgcgccga ggtcgaaaga ttgctcctcg atcgcctagc
     8881 gcacttcaag gaagaattgc gcggcgtcac cgcaggtcgt ttggtgcaga ttgcgctgtt
     8941 cgatcacgag ttcgaccagc gccccgccgc gcctccgctc ggcaccttgg cttggaagga
     9001 ttggcgggga gagcccgtgc tctccttccc tgccttttcc gaacggagct ccgttgctcc
     9061 gccttcgcct gaagcgcgcg cccataccgc cgtcgaacac tcgagcgtga cgagcgctcc
     9121 atcgaccgcg agcagctccg tcgctccaac gagcgaggca catgtatcgg gtcaaggcag
     9181 tgccagctcg gtatctaccg gcgcagcacc agctggcgcg gcgtcaccaa ctgccgccga
     9241 ttcgagcgcg cccgctcctg ctgagcctgt ggggacaggt tccgtcgaag aagcggccgc
     9301 gagcgacgcg ctgactctca acaatgtggc ttcgtcgagc ggcagtgcgg tggccaccac
     9361 cggctcggtc gaccgcgcga cttcgggcgg agaggccgga gaaaagatcg cgacggagaa
     9421 cccaacccgc gagactccgg ccgaaaccga tgcgctggcg gagaaaccgc gcgggcacga
     9481 cgcaaccgag catgaagtct ctgcgctcgc tccgccatcg ccggcggcag cacccgagat
     9541 tgcgcccccg aacgcggcaa gcgagtcgaa tcagtccctt gcccctgtcc cgagtgaagc
     9601 gcccccggcg acggatgcgc gcgcgaacga gtcggcgcga ccttcgtcgc cggtcgagct
     9661 gcgcgactcc aaaccctcac tcggctctgt cgaacgtcct tcacgcggcc gcatgcgcgt
     9721 cgggagacgc cgcgcgggag aggacctgat cagtgagttg ttcgaggtga tgcacgagct
     9781 tcacttcgcg catgacctga tgaccggcac ggatttccta ttgggaacgc tgctcgactt
     9841 gatcccgtgt gaagtcacga tgctcgaggt tttcgacatc aacacgcgac agttcgtggt
     9901 ggtgcgcggg cagggcggag gcgcgagcag tacggtgctt cagcgcacga gtgacaccga
     9961 tccactggtc ggcgagatca tgcggcgcgg ggcttcgcgg gtattccgcg ccaccaacga
    10021 cgctcgtttc cacacgggcc gcttcgggaa attgacggcg cacttggagc aggtgttgtg
    10081 cggcccggtt cgtcagggcg gtcgctatct cggtctaatc gagctcggca atccgctcgg
    10141 cggcgcccca ttccacgagg gtgaagtgaa cgcgctcgac tacatctgcc agcagtacgc
    10201 ggagttcgtc gcgagccgcc cggtcgtgct cgattcggat gtgatcctgg gccgctgatg
    10261 gccgatcgcc ctcggcgcga acgctggctc acgttcttgc tactgccggc catcgtggtc
    10321 gcagtgctgg tgttggtttg ggtcatgttc cgctcgagct ttcagctcga aaagctgcgc
    10381 gaacagtcgg tgatcgaggc cacgctgctg ctcgccaacg agaaggccga ccgcctcgac
    10441 aagcgcatca tcgagcagga caacgtgatc cgctccacgc tggcggtgga tgagcgcgaa
    10501 gatttcgggg cccgctggcg agaggccgcg cagctgcaga cgcccacggt gcgcgccgta
    10561 ttggtgttgg atctgagcgc gcccgacaag cccgtggtgg cgatggcctc gcgcgccccg
    10621 ggtccggaga gcgagcgttt ccggcggctc ttggtgcacg ccatgctgcc cgatctgaag
    10681 ctcgagcggc ctccactaga agagctgcga catctccaca agacctatcg cgggcagaat
    10741 tacctgcttt cacattggga gaccgagagc gcgggccgcc gctatctgac ggtggtgtgg
    10801 cacgatgtgt cgcgcatcgt gcacgacgtg ttccccgagc tgtacgccga tgcccagcaa
    10861 agccgcgtga acgtggtgga tgccgagggc cgcatcgtgt ttggcccggc gcaaagccgt
    10921 ggcggcctca cgctcgggcg tcagttcgag accacactct ataagtggac cttgaacgtg
    10981 accatgaact cggccgaaga gctcgcgacg gccgtggcac ggcgaaggct gctcgaaatg
    11041 gcgctggtcg gcttgtccgg cgtggtcgtg attctgggca tcatcgtgat cgtcgtcgcc
    11101 gccgagcgcg agcgaaagct ctcgaacctc aagggcgact tcgtcgcgaa cgtgtctcac
    11161 gagctcaaga cacccctctc actcgtgcgc atgttcgggg aactgctgca gtccgggcgc
    11221 gtcgacagcg aggacaagcg caagcaatac ctgcagatta tcgtgtccga gagcgaacgc
    11281 ctgggtagcc tgatcgagaa cgtcctcgac ttcgccaagg tggagcgcgg caaggctgcc
    11341 tacgagttcg tggaggccaa cctgaccgac gtggtcacgc gcgcggtgga ggcttgccga
    11401 ccgcgcgccg agcgggagca ggtcgagatc gagacggaat ttccggatgg ccctcagctg
    11461 gcgtggatcg acgagcgagc catcgagatc gccgtgatca acctggtcga caacgcgttg
    11521 aagtacgcgc cgggcggcaa gcgcgtacaa gttgcggtgc gtcggcgacg cgatcacctg
    11581 gaaatccgcg tcagcgacca aggcccgggg atcgcgccgg aggatcgcaa gcgcatcttc
    11641 gagcgcttcg tgcgcggtca gagcgcgcac ggcaagcaag tgcgcggcag tggcatcggc
    11701 ctcgcgctgg tgaaacacat cgcgcaggct cacggtggcg cgtgcgacat cgaagatggc
    11761 acccccgtcg gggcgacgtt cgtgttcacc ctctcgatgg cgccgcgcgc tcgtcatcga
    11821 ttgcttccct tcagttcacg tgccacgagc tgaagccgac gccaaccccg gcgcgccgcc
    11881 cgcgcagccg cgccgaaccc gccctgcgac tacgtcctgc ccacgcggcg actagccccg
    11941 cgaatcggcc aattctcgcc gccaatagga caccgctgct ccaaccccga gcacggccgt
    12001 agtcgcaatc agcaccaagg caaacctatt gctgctcaac acgagggcca cgcacgcgaa
    12061 ctgcagcacc gtcacggctt tgcctagcca gttcgctcga ggatcttcgg ccttcgcgcg
    12121 gcgccgctca cgatggagcg cccaccacac caccaggggc agctctccga actcacgggt
    12181 cccgagcagc agcgccgata gaggagagag ctgggcccgc gcgatcaacg taaccagcac
    12241 ggtcagcgcg aagatcttgt ctgtaacgcc gtccacgacc gcgcccgtcg gcgttgccat
    12301 gcccaagcgt cgcgccaaga agccgtcgag cacgtccgta aagccagaca cggccaagat
    12361 gacgagcgcc gtggatgtct cccgcacatg ctcgacaaac gcgtacgcca acggaaggcg
    12421 cgtgaagctg acgaggttcg gccagagcag caaatctttg gcgcgatagg cccccatccc
    12481 tcacccctcc ctactcggcg acctcgccct cgtcactcgc gccttgcagc tcgagcaact
    12541 ccttgtccgt tagataaacc gagctgcccg gcttcttcgc cggggcttgg ggcgacggcg
    12601 cagcatcctc ttcatcacca gaatccactt caccagccgt cgccgctgct gcggacggcg
    12661 tgggccccgc ggcaacggca ggctcggtag gcttttccgg tttgggctcc gcgcctttgc
    12721 tggccaccgc ctctgcgggc aaggaagcgg gcaacggcag ggcgtcgagt tgcgcatcgt
    12781 acttcgcctt gagctccgcg aacgcggcgc ggctctcccg ggagagcgtg cgcagcgcgt
    12841 ccatgttcag cgtcatgggg tcgatgaact caccattttt tcgggcactg aagtgcaaat
    12901 gcggccccgt cgatcgaccc gtggagccaa cgtatccgat gatctggagg cgcttcactt
    12961 tatcgcccac cttgagcccc tcggcgaagc gcgacaaatg cgcatacccc gtttcgatgc
    13021 cgccgccgtg ctcgatcttc acgaggttcc ccgtcggtcc gatgtagctc gcgtggctta
    13081 cgacgcccga cgagctcgcg ccgacggggg tgcccatcgg tgagccaaag tcgatcccga
    13141 ggtgcggcat gaccttcttc aggatcggat gcatccgctt caaattgaag ggggatgtca
    13201 tcggggcatc cttgatgggc ttacggaagc ccccctcgta gggcgcgtgg ccctccgcgt
    13261 cgtagtagcc gcgctctttc gggttatcga agtagtagag tcggaagggt ttttcgtccg
    13321 gaccgtcttg aatttcgagc gcctcgaccc cggcgtaacg cgcaaactca ccgagcaccg
    13381 tgacctcttg ggcaatcacg cgcagccgtg caccgcgcga gatctcatcc atggtcatgt
    13441 gcccatcgag cgccttggcg accgcgcgcg ccaggcccgc ctcgaagcca ccgcgctgcg
    13501 cactggcgtc gaagctcttg ccgtcgtgca cgaacgagcc gcggatgggg ttatgggcga
    13561 ctttgagatc gagcttgctg cccttgagtc gcccgccttc gccctcgaga gattgataca
    13621 cctcctcggg tgtcaccaca tactcgaaac ctttgacgcg cgagctcgcg cggtcaatcg
    13681 ccaccacgaa gcggtccgac ttcttgcact tgtcgaagtc gcgcacgcct ttcatcgagg
    13741 cgacgatgcg atagttctcg cgcatctcga cgcccgcttt ttcgagcgcc ttcagaaagg
    13801 catccgtgcc aattttgcct tcgacgatgc gcacggaggc gtcgctgcgc atatcgccta
    13861 cgcgcggtgg tcccttctct ttcgcgcgct cgcgtttcgg cgcgggcggg ggcgcgggct
    13921 cctgcgcgct cggtgcttgc ggcgggggcg cgctagtcga agtcggtagg gtgaccggca
    13981 ctttgagatc gacgttcatc ccgagcgcga ccagcgagct aacggtagcc acgcccacgg
    14041 cagtgccgat cagtgcgatc caattggggg acaggcgcga gcgtcgtgga ggcgggctgg
    14101 tcgccgagaa gggcagcggc gcgaacggcg acactccggg aggctctgcc acgggttggg
    14161 gagcaggccg ttccacgact tcttcatcga agtcgccaat ttcatcgagg tcaccgagcg
    14221 ccgcttcgcc ttgcgcgcgg cgagtcgcct cgagccgctc gagcagcacc tctcgtgcgc
    14281 ggcctccgcg gggtagaccc gattgcacac gctccaccga gcggcccgcg tcgccggagc
    14341 tcacgagctc ttctaccggg agttccgatt gccctgcagc ggcgtcccac tccggctcga
    14401 catgttctgg ttcgaggcga ctcggctcgg ctcgatttgg ctctgttcga tttgattcgg
    14461 gctccggcgc gcggcgctct cccccgccaa gctcggcggc cccgccctga gcagcgggtt
    14521 gctcccgctg gggagtggag tcactcgatt cggatttggg tgccggattt ggtgccccca
    14581 gctcggactc gaatgccggg tcggtttcac cgtcttcgcg caccgatggg tcgatcactc
    14641 ggccaacatg gcgcgaacct ccaggaacct gtgacgacgc cacggaaggc cgtcgagtag
    14701 cagcgccttg ccggctcggc aagcactccg gctcacccag ccagccggtg tcacaatttt
    14761 gcagttcggt tcttcgcggg cgccgcatcc ctcgaactgc gactggccct cgtagccgca
    14821 agctcttatt gagggacccc gcatgcggca gccctccccg ccctccgcca cgcctcggac
    14881 cggtttcgcg aaccgtgtga aactcgcgcg ctggccgcgg ctgcgtagcc tgatccagct
    14941 gctgcgcgga cgaggctcgc gcaaacagag cgcttggagc gtgggggtgg gcttgttcat
    15001 cggctgtttg ccgctgtacg ggctgcactt tccgctgtgc gtcgcggtct gcgtgccttt
    15061 cgggttggac gccctgatcg cctacctggc ggcgaacgtt tcgaacccgc ttttgctgcc
    15121 gctgatccta ctggccgagg tcgagatcgg ctcctggctt ctgcacgggc aatctgcgga
    15181 gttcacactc gatgccgcac gccgcctggg tgctttggga tttggaaccg aggtcgcgct
    15241 gggcagtgtc gtgttcggag ctgctctggc gctcttgggc gcgctgctca ccgcgcgctt
    15301 cgttcgcgat cgtcccccga cgcccctcga gctcgcgctg cgacgcacgc aagctcttta
    15361 caccggagcc cccgccgcgg accgtcacta cgtgcgcttc aagatggcgc tcgatccggt
    15421 gatcgcgcag gtcgcggagc tggcagcacg ccacggcccc gcgggctggg ggcgcgtgtt
    15481 agatgcgggc tgtggacgcg gtcaacttgg cctgctgctc ctcgaactag gggccgccgc
    15541 ggatctcacc ggcttcgact tcgatacgcg caaggtggag gtcgcgcgcg gggccctggc
    15601 gcacgccagc gccaacggcg ggcgggcccg tttctatgtc ggcgacatcg cccaagccga
    15661 gctcgcgggt ttcgatacga ttttgctaat cgacgtgctg cactatttgt cgcgcgacga
    15721 gcaagaccgg ctgctgacgc gcgtcgcgcg cgcgctgccc gtcggaggtc gcctgttgct
    15781 gcgtgaaaca gactcacgcc gctcggcaag cagcctgctt tccaagagtt tcgagtatct
    15841 cgcgacccgc gtggggtaca accgcagcgc ccaccggttg gctttccgct cgctgcccga
    15901 gctcactcgg tacatcgaga acctcgggct gaagtgcgca cagatcggcg cggagcacgg
    15961 tgtcgcggag caagccggcg agcacggtgt cgcggaaggg cgattcaccg aggggcaagg
    16021 tacgttgagc aatgagcttc tcttggtgga gaagactcg
//
LOCUS       DASTKU010000844        11813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_834270, whole genome shotgun sequence.
ACCESSION   DASTKU010000844 DASTKU010000000
VERSION     DASTKU010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11813
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834270"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VFQ61_29570"
     CDS             <1..1033
                     /locus_tag="VFQ61_29570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HET9958693.1"
                     /translation="DGFELAELMRGTERTRHIPIIFVTAGAWERNRLFRGYDAGAVDF
                     LFKPIDPHILRHKVRTFVALYRQRVSIREQVRALERSEAEARALKDELAATLRLNETF
                     VAAVSHDLKNPLNAVLMATELLLAGGPTPETRRIVERARNSSRRMLNMIEQLFDLSRA
                     RLAGGIPLVLRENTDLSRIAEQVRLELNLVNADCPVSLHVIGDPCGTWDDVRLERLIS
                     NLLGNAVRHGVRGAPIEMSIDGSAPDVVRFVVTNSGQIPPEILPRLFEPFSEKEPSRR
                     RGDGLGLGLYIVAQIVSAHAGEIDVRSGDGTTTFTVSLPRCAPRTTPRLNEPALLDLS
                     PDSEPGSRR"
     gene            complement(1186..1716)
                     /locus_tag="VFQ61_29575"
     CDS             complement(1186..1716)
                     /locus_tag="VFQ61_29575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958694.1"
                     /translation="MKSLISVVCVASALAFACQKSPEKQAEELREAERNAEQKTVEAR
                     QEADVKAAKAYEEVDRTAANQNEKVADKAQDVNDSLMTAREDLRKDIEGDLNSIDKKL
                     VDLRTKLNNSKTVKTSRTEMDASIRLLKDQSEALRRDMSQIQTSSADAFGNLKSNLKS
                     RVDNLEKSVDDIDNKM"
     gene            complement(2082..2621)
                     /locus_tag="VFQ61_29580"
     CDS             complement(2082..2621)
                     /locus_tag="VFQ61_29580"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET9958695.1"
                     /translation="MSRAFVKEDSQTEAPVMAHRAPLPPGVPNYVTPRGLAHLREELV
                     DLLAELQTAEPVELPEAHRARSLALMTRKLELEERLASAVLVERTPENQGEVRFGANV
                     TVRIDAHSSSAAKTRVYQLVGADEADPQQGRIAFFTPIARALIGRSVGDEVQIQTPRG
                     EETLAIISVNYDSSPSSSR"
     gene            2718..3710
                     /locus_tag="VFQ61_29585"
     CDS             2718..3710
                     /locus_tag="VFQ61_29585"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HET9958696.1"
                     /translation="MKESTRSFYVSLVQHTVARILGCMDEALDLGELAREQALSPLHL
                     HRILRGLLGETPLELHRRLRLERAAWRLVHTTRTVTRVAFEAGYETHESFTRSFREAF
                     AVSPSEYRARGSARGEGATCAPPISFILPAASGIHFVPEGASAALSASSMERTEAELL
                     QIPWLTGEQLMQIDVQWMPEMRVAAVAHRGPYNRISEAFAKLGAVAAREGLLANRTEA
                     PKMIAIYYDDPESTPAAELRADAGLSVEPEAVIPDGLHTIVVPPGRYARAVHVGPYSQ
                     LGDAWARFLGQGLPQSGYRVGSGPSYELYVNNPQNAQPAELRTELYVFVEECPK"
     gene            3818..4459
                     /locus_tag="VFQ61_29590"
     CDS             3818..4459
                     /locus_tag="VFQ61_29590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET9958697.1"
                     /translation="MNSPLSEPAGAEALPERAKPQLVAPEGDLTPIVGANVRRLRLKR
                     GLSLERLAKSSGVSRAMLGQIELGQSTPTINILWKIARALDVSFSALVADATAQNAVI
                     LKRSNARLLRSSDGSFTSRALFPVNASRSTEFYELHLAPGCLERGEPHPPGTLENLVV
                     ASGALQVSVGTRRYSLEAGDAITFQADVAHEYLNSAREPAVVYLVMTYAERAP"
     gene            4565..4885
                     /locus_tag="VFQ61_29595"
     CDS             4565..4885
                     /locus_tag="VFQ61_29595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET9958698.1"
                     /translation="MKEAQALLGTGELEVIDVREPHEWLTGHIEGARLLPLGTFRQNP
                     KAAALGDAVLFVCAAGVRSETAARLALSHGIKRVFNLVGGTRAWAGAGLPLVREEYAS
                     TAAE"
     gene            complement(4958..7018)
                     /locus_tag="VFQ61_29600"
     CDS             complement(4958..7018)
                     /locus_tag="VFQ61_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment cargo
                     protein cysteine desulfurase"
                     /protein_id="HET9958699.1"
                     /translation="MSTSGYDSGVSAPPSATDSGAGEALTHEIARLANQFFGQALPPP
                     ANPADPTSPVAAAPVGSAPIISAAPRVNAVHPTSLVGTAQPSPVSPPSRIAPSAFSTG
                     AGGLSTAATLPPTPGALIAPPLASAPPPRESDLRGGLNSALDAIGIPNLALFDSSPSS
                     DTPYYLQHAGVPGAVPAAVSSGVAPHAGLASTNGAPPVSAPLAAGWAAPAAPALATPT
                     APVGAAPALPGLAAPTITPLPSTASASPQSGTSPLGVGSTAPASPLSSGFTFQPEVVP
                     LAQRHGGILDPVSVKRDFPILNEHVHGRPIIWLDNAATTQKPKSVIDRLTHFYEHENS
                     NIHRAAHTLAARATDAYESGRNKVARFLNAPSVAEIVFVRGATEGINLVAQSWGRRNI
                     GPGDEIVITWLEHHANIVPWQQLAAEKGAVLRVAPVDDRGDIILEEYERLFNSRTRLA
                     SITHVSNALGTIVPVKTMTEIAHRHGACVLVDGAQAVSHQRVDVQDIGCEFYVFSGHK
                     VFAPTGIGALFGKRAVLEAMPPWQGGGNMISDVRFERTSYQPPPARFEAGTGNIADAA
                     GLGAAIDYVEQLGLERIAHYEHELLEYGHSRLLEVPGLRLIGTSATKAGVLSFVIDGI
                     KTEELGAALDRYGIAVRAGHHCAQPILRRYGLESTVRASLAPYNTRDDLDALVAALRR
                     LTGR"
     gene            complement(6999..7943)
                     /locus_tag="VFQ61_29605"
     CDS             complement(6999..7943)
                     /locus_tag="VFQ61_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment shell
                     protein"
                     /protein_id="HET9958700.1"
                     /translation="MADSGQAVQTLGAAAARQLANTTKTPPQWVGTTPRWLVSLLPWV
                     PVEAGTYRVNKVKEADTSGIGIECSPSDGDELPHAFVDYEESPREYTLSTVTTTLQVQ
                     TRISDLYRSPMDQVREQLGVLVDMVKERQENELVNNVNYGLLSHVVDSMRVSTRAGAP
                     TPDDLDELAAKVWKEPAFFLAHPRAIAAFGRECTRRGVPPPTTTLFGSPFLTWRGIPI
                     IPSDKLHIKEGEDGRATTNILLLRTGERKRGVVGLFQPGVPGEVTPSLSVRFMGINQR
                     GAASYLISLYCSAAVLAEDAVGVLENVQIDRYHEYKWL"
     gene            complement(8107..9642)
                     /locus_tag="VFQ61_29610"
     CDS             complement(8107..9642)
                     /locus_tag="VFQ61_29610"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958701.1"
                     /translation="MTLVLFDTARAARVPFPVDVPHELGIYSCGPTVHGRKHLGELRQ
                     YVVVDVLKRRLASMGRRTRHVINITDVGHLTDDADLGEDRVLRAARRAGESVTQTARH
                     FTRLFQRDLALLGVSSPQVWATASEHVLEQIQMAVELERRGLTYLTKDGLYFDTSRAP
                     GFGKLARASGIRTQPQLRIVAADDRRHPDDFLVWRRKDAETECASDHDEGNEAQSSRS
                     KTFDSPWGRGIPGWHVECAAMAQLYAGPQIALHTGTTDHIHSHHDHELAQAEQAFDGK
                     PWVRHWLHVAPVLVASEQPDQDGATRTMADHAKNSITVDDVMERGLAPRICRLLLLST
                     HYRSKLVVSWSVIATVASQYERLARLVAELKPVQAEPGHTHAVSRNPLVTRLKRLFER
                     AIDDDLQTPRALSVLWETLKHPLLSAADKLTVLSHIDSTLGLGLLEAASLLEVPASPS
                     SLTLPIMQLIDRRNEARNLQDFALADALRTELQRLGFNVEDHATGTRIRARSLTPGKA
                     EAS"
     gene            9859..10209
                     /locus_tag="VFQ61_29615"
     CDS             9859..10209
                     /locus_tag="VFQ61_29615"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9958702.1"
                     /translation="MEGQPLQSTEEILARSEWDSPDSGLLHRTQPVLTGKEKALLEYF
                     EKHPNEVLPRALLLQEVWGTVYTGGPRTVDVHVCRLRAKLRDRVALITLRGRGYQLLS
                     SSPLWSDTEAASAG"
     gene            complement(10256..11026)
                     /locus_tag="VFQ61_29620"
     CDS             complement(10256..11026)
                     /locus_tag="VFQ61_29620"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET9958703.1"
                     /translation="MGARKKHQPATPRTQDGRSHESRSLESRAQAPERSRVPDATADE
                     LHIGHRDAARTRARTNYEAGDELGAAELARRVAESLRRFRAERQLSLDELSARCGVSR
                     AALSQIEGCRTNPTLAVLWKIAVGLEVPFHDLLESGTDESVLVLRAGDASPLRSADGR
                     TESRLLSPGGSRTDTEIYELRLIPKAILKSEPHARGTTETVIVLTGAMRLSVGGTDYD
                     LSVGDSVYFNADVAHSYENRSSRETRIMDVIHYTKGRG"
     gene            complement(11099..>11813)
                     /gene="epsC"
                     /locus_tag="VFQ61_29625"
     CDS             complement(11099..>11813)
                     /gene="epsC"
                     /locus_tag="VFQ61_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HET9958704.1"
                     /translation="DYFVGDTLGSTLRELHTQVGRELQFVASQRGEHAREGAAEELTQ
                     QFAGRLPHVHAALETDVRAAYEGDPAARSLDEALFCYPGVTALLHHRLAHELYALGAP
                     LLARILSEIAHSTTGIDIHPGARIGRSFFIDHGTGVVIGETTVIGDRVRVYQGVTLGA
                     KSFPLDEKGLPLKGLARHPVVEDDVIVYAGATILGRVTIGRGSVIGGNVWVTRSVPPG
                     SRITQAQARTEAFDGGAGI"
BASE COUNT         2109 a         3586 c         3815 g         2303 t
ORIGIN      
        1 agacggcttc gagttggccg agctgatgcg aggcaccgag cggacacgcc atatcccgat
       61 catcttcgtg acggccggcg cttgggagcg caatcgactg tttcgtggct atgatgcagg
      121 agccgtagat ttcctattca agccaatcga tccccacatc cttcgtcaca aggttcgcac
      181 cttcgtggcc ttgtatcggc agcgagtatc gattcgcgaa caggtccgcg ccctggagcg
      241 gagcgaggcc gaggcccggg cgttgaagga tgaactcgcc gccactttga ggttgaacga
      301 gacgttcgtc gccgccgtca gccacgatct caagaaccct ctcaacgcag tgctcatggc
      361 cacggagctg ctgttggcag gcggcccgac cccggagacc cgccgcatcg tcgaacgagc
      421 gcgcaacagt tctcgacgca tgctgaacat gatcgaacag ctcttcgact tgtcgcgcgc
      481 gcgccttgcc ggcggcattc cgctggtttt gcgagagaac acggatttat cgcggatcgc
      541 cgagcaggtc cgcctcgaac tcaatctggt caacgccgat tgcccggtgt cactgcacgt
      601 gatcggcgat ccctgtggca cgtgggacga cgtacggcta gagcgactga tttcgaatct
      661 gctcgggaac gcggtccggc acggcgtccg cggcgctccg atcgagatga gcatcgatgg
      721 aagcgcgccg gatgtcgtcc gcttcgtcgt caccaactcc gggcaaatac cgcccgaaat
      781 tttgccacgc ttgttcgaac ccttcagtga gaaggaaccc agccgtcggc gcggtgatgg
      841 cctggggctc gggctctaca tcgtcgccca gatcgtctcg gcccacgccg gagaaataga
      901 cgtgcgcagc ggggacggaa ccacgacctt cacggtgagc ttgccgcgct gcgcgcctcg
      961 cactacccct cgactcaacg aaccggcttt actcgacctc tcgcccgata gcgagccagg
     1021 gtcgcgacgc tgagcgccgc ttttgggcaa acgaagtgat ggagcaaata gacgcaaggc
     1081 cggtgatcgc gatcgcgatg ccgaccttgc gagagcacct tgggcggagc cccaagaacg
     1141 ctcctattcg cgagtggtgc cgctcagcgg caccgtgaga gtcgttcaca tcttgttatc
     1201 gatgtcgtct acggacttct ccaagttatc cacccgggac ttgagattcg acttcagatt
     1261 tccgaaagcg tccgcggagc tggtctgaat ctgactcatg tctcgtcgta gggcctcgga
     1321 ttggtccttc agcaaacgga ttgaagcgtc catttcggtg cgggacgtct tcaccgtctt
     1381 ggagttattg agtttggtgc gcagatcgac caatttcttg tcgatgctgt tcaagtcgcc
     1441 ttcgatgtcc ttgcgcaagt cttcgcgcgc ggtcatcagc gagtcattca cgtcttgggc
     1501 cttgtcggcc accttctcgt tctgattggc ggcggtacgg tcgacctctt cgtaggcctt
     1561 ggccgccttc acatcagctt cctggcgagc ctccaccgtc ttttgctcgg cattgcgctc
     1621 cgcctcgcgc agctcttcag cctgcttctc gggcgacttc tggcatgcga aagcaagggc
     1681 gctggcaacg caaaccacag agatgagtga cttcatatga attctccttg ccggctcaca
     1741 ccaggctgtc gaatatcgat attcgaaatc ccaggcttgc ggcacacgag ccctcatagc
     1801 caactgcgtg ccacggccat gcgtcgtgga cgaccaccgc taaatagcgg aaaagcagca
     1861 gaaacggacg ctcggacgta gagagttgcc ccaataacgg tcgcgcaccg gtcggcctcg
     1921 catgtggctc ttgtgcagaa ccgcacattt gatcgcgaca atggcaaaaa cgcggcagaa
     1981 gtcaggcgcc ggaggtcggc caattccgcc atggcgggcg cggtgttcgc taccaaccat
     2041 ctctacgggc ctcgagtctg cggccggagc cggaacgacg cctagcgcga cgagctcggt
     2101 gaggaatcgt aattcacact tatgatcgcc aaagtctctt caccgcgtgg cgtttgaatc
     2161 tgcacctcgt caccgacgct gcggccgatc agagcgcgcg cgatgggggt aaagaaggcg
     2221 atgcgacctt gctgcggatc cgcttcgtcg gcgcccacga gctggtacac gcgtgtcttc
     2281 gcggcactac tcgagtgcgc atcgattcgc accgtcacgt ttgctccaaa ccgcacctca
     2341 ccctggtttt ccggagtgcg ctccacgagg actgcgctcg cgagtcgctc ttcgagctcg
     2401 agtttgcgcg tcatgagggc aagcgatcgg gcgcggtgag cttcgggcaa ctcgacgggt
     2461 tcagcggtct ggagctcagc caagagatcg accaattcct ctctcaagtg ggcgagccca
     2521 cgtggggtca cgtagttggg tacacccggg ggtagaggcg cccgatgagc catcaccggc
     2581 gcttcggtct ggctgtcctc tttgacgaat gcgcggctca cggggtcgag tctgacacgg
     2641 cgtccggaaa aatcgcaaac ggcgtcaagt gatgccagct gtgctcggct actctccctt
     2701 cccgaaggac ggcagatttg aaggagagca ctcgctcgtt ttacgtttca ctggtgcagc
     2761 atacggtggc ccgcatcctt ggttgcatgg atgaggcttt ggatttgggg gagctcgcgc
     2821 gcgagcaggc gctatcccca ttgcaccttc accgcatcct ccgcggcctt ctgggagaga
     2881 cgccgctcga gctgcatcgg cgtctacgct tggaaagagc ggcttggcga ttggtccaca
     2941 ccactcgcac ggtgacgcgg gtcgcattcg aggctggcta cgagacgcat gagtcgttca
     3001 cgagatcctt ccgggaagcc tttgcggtct ctcccagcga gtaccgcgcc cgcggcagcg
     3061 ctcgcgggga gggcgcgacg tgcgctccgc ccatctcgtt cattttgccc gcggcttcgg
     3121 gcatccactt cgtgccggaa ggcgcctccg ccgccctgtc cgcgagttcc atggagcgca
     3181 cggaggccga gttgctccag atcccttggc taacaggaga acagctaatg cagatcgatg
     3241 tacaatggat gcccgaaatg cgagtcgctg cggtggccca tcgcggcccc tacaaccgca
     3301 tttcggaggc cttcgcgaag ctcggggccg ttgcagctag ggaggggttg ctcgcaaacc
     3361 gcaccgaggc cccaaagatg attgcgatct actacgatga tcccgagtcg acgcccgcgg
     3421 ctgagcttcg cgcagacgcc ggcttgagcg tggagcccga ggccgtgatc cctgacgggc
     3481 tgcacacgat cgtcgttccc cccggtcgtt acgctcgcgc ggtgcacgtg ggcccctaca
     3541 gtcagcttgg ggacgcgtgg gcgcgcttct tggggcaagg attgccgcaa agcggctacc
     3601 gcgtgggttc ggggccttcc tacgagctct atgtcaacaa tcctcagaac gcgcagccgg
     3661 ccgagcttcg gactgaactg tacgttttcg tggaggaatg tcccaaataa aagactgggg
     3721 tcgcccggcg cagggctgac gtcgagcagc agtcgggaaa cttagtctac tggttcgatg
     3781 ggaattgtgt atattcggag cctgcttgag gcttctgatg aactctcccc tgagcgagcc
     3841 cgccggtgcc gaggcgctcc ctgaacgagc caagccgcag ctcgtggcgc ctgaaggtga
     3901 cctgacgccg atcgtcggag cgaacgtccg tcggctacgt ctgaagcgag ggctgtctct
     3961 ggaacgtctg gcgaagtcat ccggggtgag ccgcgcaatg cttggtcaaa tcgagctcgg
     4021 ccagagcaca cccaccatca acattctgtg gaagatcgcg cgagcgttgg acgtgtcgtt
     4081 ttcggcgtta gtggccgacg ctacggcgca aaacgccgtc attctcaagc gctccaacgc
     4141 tcggctgttg cgctctagtg acggatcctt cacctcgcgc gcgctgtttc cggtgaacgc
     4201 ttcgcgcagc acggagttct atgagctgca cttggcaccc gggtgcctcg agcgcggtga
     4261 accccatccg cccggtacgc tcgagaactt ggtggttgcg agtggcgcgc ttcaagtctc
     4321 cgtcggtacc cgtcgttact cgctcgaagc cggtgacgcg atcacgttcc aagccgatgt
     4381 ggcacatgag tatctgaaca gtgctcgcga acccgcggtg gtctacctgg tcatgaccta
     4441 cgccgagcga gctccttagc cgcggcaaac cgtacaggat cgaagaatag tctagttttc
     4501 tgatagatta tatggaagtt tccggaaggt gagttagact gtgagcgtgc tccaaaaagt
     4561 atctgtgaaa gaagcgcaag ctctactcgg tacgggtgag ctcgaagtca tcgacgtgag
     4621 agagccgcac gagtggctaa ctgggcacat cgaaggtgcc cgtctgctgc ccctcggcac
     4681 tttccgtcaa aacccaaaag ccgccgctct cggagacgcc gtgcttttcg tttgtgccgc
     4741 gggtgtacgc agtgaaacag cggctcgctt ggcgcttagc cacggtatca agcgcgtgtt
     4801 caatctcgtc gggggcaccc gcgcctgggc gggtgcgggg ctaccgctgg ttcgcgaaga
     4861 gtacgcctcc acggccgcag agtagctgtg tgtctccgat aatggactga ctggtcgccc
     4921 aactccgttc cgtgcgagcc cgccaagcct gcatcggtca acgcccagtc aggcgtcgta
     4981 gcgccgcgac gagtgcatcc agatcgtcgc gtgtgttata gggggcgagg gaagcgcgca
     5041 cggtgctctc cagcccgtag cgccgcagaa tgggctgggc gcagtggtgg ccggcacgca
     5101 ccgcgatccc gtatcgatcc agcgcggccc caagctcctc ggtcttgatg ccatcgatca
     5161 cgaacgagag cacgccggcc ttggtggcgg aggtcccaat gaggcgcaag cctggcacct
     5221 cgagcagtcg agagtgaccg tactcgagca attcgtgctc gtagtgtgcg atgcgctcga
     5281 ggccaagttg ctcgacataa tcgatggccg cgccgagccc agcggcgtcg gcgatattac
     5341 cggtccccgc ttcgaagcgg gccgggggag gctggtatga ggtccgctcg aaacgcacgt
     5401 cactgatcat gttaccgccg ccttgccaag ggggcatggc ttcgagcaca gcgcgtttgc
     5461 cgaacaacgc gccaatgcca gtcggggcga ataccttgtg gcccgaaaaa acatagaact
     5521 cgcagccgat gtcctggacg tccacccgct ggtggctcac cgcctgcgcg ccgtccacca
     5581 atacgcaggc accgtggcgg tgggcaatct cggtcatggt tttgaccggg acaatcgtgc
     5641 cgagagcgtt ggagacgtgg gtgatggacg cgaggcgagt gcgtgagttg aagaggcgtt
     5701 cgtactcttc caggatgatg tcaccgcggt cgtcgacggg ggctacgcgc agcacggcgc
     5761 ccttctccgc ggcaagttgt tgccaaggca cgatgttggc gtggtgctcg agccaggtga
     5821 tcacgatctc atcgcccgga ccgatgttgc gtcgccccca gctttgggcg acgaggttga
     5881 tgccctccgt tgcgccacgt acgaatacga tctcggcgac agatggtgca ttcaaaaacc
     5941 gagcaacttt gttgcgtccg gactcgtaag cgtcggtggc gcgcgcggcc agggtatgcg
     6001 cggcgcgatg aatgttcgag ttttcgtgct cgtagaagtg cgtcaatctg tcgatgacgg
     6061 acttcggctt ttgggtagtc gccgcgttat cgagccaaat gatcggcctg ccgtgtacat
     6121 gctcattcag aatcgggaag tcacgcttta cgctgacggg atcaagtatt ccgccgtggc
     6181 gctgagcgag cgggacgacc tcgggttgaa aggtgaatcc agagctcaga ggggaggcag
     6241 gtgccgtcga gcccacgcca agcgggctcg tgccggactg gggcgaggct gatgcggtgg
     6301 atggcaatgg ggtgatggtc ggtgctgcta gaccaggcag ggccggtgcc gccccaacgg
     6361 gtgctgtcgg cgtggcaaga gcaggtgccg cgggggcagc ccaacctgcg gcgagcggag
     6421 ccgaaacagg cggcgcaccg ttcgtggaag cgagccccgc gtggggtgca acgccggaag
     6481 agaccgccgc gggtaccgca ccggggactc cggcatgctg tagatagtag ggtgtgtcgc
     6541 tgctcgggct cgagtcgaac agggccaagt tcggaatacc gatcgcgtcc aaggccgaat
     6601 tgagcccgcc gcgtaggtca ctttctcgag gcggcggggc cgacgcgagc ggtggcgcga
     6661 tcagggcgcc cggcgtaggc gggagcgtgg cggccgtgga aagcccgccc gctcccgtcg
     6721 agaaggcgct tggagcgatg cgcgacggcg gcgataccgg cgacggttga gccgtaccaa
     6781 ccaaggatgt ggggtgtacc gcatttaccc gcggcgcggc gctaatgatt ggcgcggagc
     6841 cgaccggcgc agcagccacc ggcgaggttg gatcggcggg gttggcgggg ggcggtaacg
     6901 cctggccaaa gaattgattg gccaggcgcg cgatctcgtg agtgagcgct tcgcctgcgc
     6961 ccgagtcggt tgcggacggc ggcgcgctca ctccggagtc ataaccactt gtactcatgg
     7021 tacctgtcga tctgcacgtt ttctagaacg cccacggcat cttcggcgag aactgcagcc
     7081 gagcaataga gtgagatcaa gtacgacgcg gcgccgcgct ggttgattcc catgaatcgc
     7141 accgagaggc ttggagtgac ctcaccgggg acgcccggtt ggaacaagcc caccactcca
     7201 cgcttccgct cacccgtcct gagcaacaga atattggtcg tggcgcgccc atcctcaccc
     7261 tccttgatat ggagtttgtc ggaggggatg atgggaattc cgcgccaggt caggaacgga
     7321 gacccgaaca acgtggtggt tggtggcggg accccgcgcc gggtgcactc tctgccgaaa
     7381 gcggcgatgg cgcgtggatg ggcgaggaag aaggctggtt ctttccacac tttggcggcg
     7441 agctcgtcga gatcgtccgg tgtgggcgcg cccgcgcgcg tggagacccg catcgagtct
     7501 acgacgtgac tcaacaagcc gtagttgacg ttgttgacca gctcgttctc ctggcgctcc
     7561 ttcaccatgt ccaccagcac gcccaattgc tcgcgcactt ggtccatcgg acttcgatac
     7621 aaatccgaaa tgcgagtttg gacctgcaga gtagtggtaa cggtgctgag cgtgtactcc
     7681 cgcggcgact cttcgtagtc cacgaacgcg tgcggcaact cgtcaccgtc gctgggactg
     7741 cactcgatac cgattcccga agtgtcggcc tctttcacct tgttgacgcg ataagtgccg
     7801 gcctcgactg gcacccacgg tagtaggctg acgagccagc gcggagtagt tcccacccat
     7861 tggggcggag ttttcgtagt attcgcgagt tgacgtgcgg ccgctgcgcc caacgtctga
     7921 accgcttgtc ccgaatcggc catttggtcc tttccagctg tcgagagtcg gtgtctcgac
     7981 gccggcgaac ctaacgcctt ggagatagca atggcaatcc ctggtgtaac ccaggagtga
     8041 gcgcggtcgc gctttcatcc agtaagttag tcagcgcctc cgttggagtg agcaatgagg
     8101 cgccggtcag ctagcttccg ccttgcccgg cgtgagcgag cgtgctcgga tgcgcgttcc
     8161 ggtggcgtgg tcttcgacgt tgaagccgag ccgttgcagc tcggtgcgca gcgcgtcggc
     8221 cagcgcgaag tcttgcaggt ttcgggcttc gttgcgccga tcgatcagct gcatgatcgg
     8281 cagggtgagg gagctcgggc tggcgggaac ttccaggagg gacgccgcct cgagcaagcc
     8341 cagccccagt gtcgagtcga tatgagagag cacggtcagc ttatccgcgg cactcagcaa
     8401 gggatgtttg agcgtctccc acaagaccga cagtgcgcgt ggggtttgta ggtcgtcgtc
     8461 gatggcacgt tcgaacaggc gtttcaaacg cgtcacgagc gggttgcggc tcactgcgtg
     8521 ggtgtggccg ggctccgctt gcactggctt cagctctgcg actagccgcg ccaggcgctc
     8581 gtactgcgag gccacggtgg caatcacgga ccaactcacg acgagcttgc tccggtagtg
     8641 ggtgctcagc aacagcagcc tgcagatgcg cggagctagg ccacgctcca tcacgtcatc
     8701 cacggtgatg ctgttttttg cgtgatccgc catggtgcga gtagcgccgt cctgatccgg
     8761 ctgttcactc gcgaccagaa cgggggcgac gtgcaaccaa tgtcgaaccc acggcttgcc
     8821 gtcgaaggct tgctcggcct gagctagttc gtggtcgtgg tgcgagtgga tgtgatcggt
     8881 ggtgccagtg tgaagagcga tctgaggccc cgcatagagt tgggccattg ctgcgcactc
     8941 gacgtgccag ccggggatcc cccgacccca ggggctatcg aatgttttgg acctggagct
     9001 ttgcgcttca ttgccctcat cgtgatcgga agcgcactcg gtttccgcgt ccttgcgtct
     9061 ccaaactaga aaatcgtcgg ggtgacgccg atcgtccgct gcgacgatcc gcagctgcgg
     9121 ctgcgttcga atgcccgaag cgcgcgccaa cttgccgaaa ccgggggcgc gactggtatc
     9181 gaaatagaga ccgtctttcg tgaggtaggt gagcccgcga cgctcgagct cgaccgccat
     9241 ttggatttgc tccagaacat gctccgaagc cgtggcccag acctgcggtg aactcacgcc
     9301 gagcagggcg agatctcgct ggaagagacg ggtgaagtgg cgagccgttt gggtgacact
     9361 ctcaccggct cgacgggccg cgcgcagcac gcggtcttcc ccaaggtcgg cgtcatccgt
     9421 gaggtgtccg acgtctgtaa tgttgatcac gtgccgggta cggcgcccca tgctcgctag
     9481 ccgacgcttc aacacgtcca ccactacgta ctggcgcagc tccccgaggt gcttacgacc
     9541 atgtaccgtc ggcccgcacg aatagatccc gagctcgtgc gggacatcca ctgggaaggg
     9601 gacgcgggcg gcgcgcgccg tgtcgaacaa tactagcgtc atgggggtaa ccctctctcg
     9661 cgtcagttgg ctgatttgga cccaaggata cgccggagca gaagatagac aagtgtctct
     9721 atcgattttg tctactttcg tggacagatc gggtgaggtg gcgtacagtg gccaacctcg
     9781 gctcgtggca gatggtccgg cttcggcgct cgaagcaggt tcgcactgac gcggccggag
     9841 acccatcgag ggatgcacat ggaaggccaa ccgctccaat cgaccgagga gattctcgcc
     9901 cgctccgagt gggattctcc ggattcaggc ttgttgcacc gcacgcagcc cgtgctgact
     9961 ggaaaggaga aggcgctgct cgaatatttc gagaaacacc cgaacgaggt gctgccgcga
    10021 gcgctgctcc tgcaggaggt ttggggaacc gtttacacgg gcggcccgag gacagtggac
    10081 gtgcacgtgt gccggcttag ggccaagctg cgtgatcgcg tggcgctgat tacgctgcgt
    10141 gggcgtgggt atcagctgct ctcgtcgagc cccctgtggt ccgacacaga ggccgcatct
    10201 gcgggttgat ggcgtctcgg cggggccatc gccccgctta ggggccccaa ggccgctagc
    10261 cgcggccttt ggtgtagtgg atgacgtcca tgatacgagt ctcgcggcta ctgcggttct
    10321 cgtagctgtg ggcgacgtcc gcgttgaagt atacggagtc tccgacggag agatcatagt
    10381 ccgtgccgcc gacgctgagc cgcatggccc ccgtgagcac gataaccgtt tccgtcgtgc
    10441 cgcgcgcatg gggctcgctc ttcaagatcg ctttcgggat caggcgtagc tcgtaaattt
    10501 cggtgtccgt tctagagccg cccggcgaaa gcaaacgact ctctgtacgc ccgtcggcgc
    10561 tgcgcagcgg gctcgcgtct cccgcgcgca gtacgagcac gctttcgtcc gtgccggact
    10621 cgagcaggtc gtgaaagggc acctcgagtc cgaccgcgat cttccaaagg acggcgaggg
    10681 tcgggttggt ccgacagcct tcgatttggg aaagcgctgc gcggctcact ccgcaacgtg
    10741 cggagagttc atcgagagat agctgccgtt cggcgcggaa gcgtcgtaag ctttccgcca
    10801 cgcggcgtgc gagctcggcc gcgccgagtt cgtctcccgc ttcataattg gtccgggcgc
    10861 gagtccgggc ggcatcccgg tggccaatat gaagctcgtc ggcggtcgcg tccggaactc
    10921 gactcctttc cggagcttga gccctgctct ctaggcttcg gctttcgtgg gaccggccgt
    10981 cttgggttct gggggtggcg ggttggtgtt tcttgcgagc gcccataccg gcgaccctct
    11041 tatcacgccc ccggccgccg ggaccacgtt tgttcgcgag aaaagacggg aaggcggctc
    11101 aaatgccggc gccgccatcg aacgcttcgg tccgggcttg ggcttgagtg atgcggctgc
    11161 cggggggaac actgcgcgta acccacacgt tgccgccgat aacggagcct cttccgatcg
    11221 tcacccggcc cagtatcgtt gccccggcgt aaacaatcac gtcgtcttcg acgactggat
    11281 ggcgagccag gcctttgagc ggcaatccct tttcgtccag tggaaaagac ttggctccca
    11341 gtgttacgcc ttgatagaca cgcaccctgt cgccaatcac cgtggtctcc ccgatcacca
    11401 cgccggtccc gtgatcaata aagaagctgc ggccaatgcg ggcacctggg tgaatgtcga
    11461 tgcccgtagt gctgtgggca atctcggaca aaatgcgcgc taacagcggg gcgccgagcg
    11521 cgtacagctc gtgtgcgagc cgatggtgga gcagcgcggt cacgccggga tagcaaaata
    11581 gcgcctcgtc caggcttctt gcggcaggat ctccttcata ggccgcccgc acatccgtct
    11641 ccagcgccgc gtggacgtgc ggtaaacgcc ccgcgaactg ttgagtgagc tcttcggcgg
    11701 ctccctctcg ggcatgctcg cctctttggc tcgccacgaa ctgtagttct cgaccaactt
    11761 gagtatgcag ctcccgaagc gtggagccca aagtatctcc aacgaagtaa tcc
//
LOCUS       DASTKU010000845         4519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_611213, whole genome shotgun sequence.
ACCESSION   DASTKU010000845 DASTKU010000000
VERSION     DASTKU010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611213"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1044
                     /locus_tag="VFQ61_29630"
     CDS             76..1044
                     /locus_tag="VFQ61_29630"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HET9958705.1"
                     /translation="MLQFGHDIITVGASAGGVQALITMLSGLPADLPAAVFIVQHLSA
                     DYQSHLPELLGRRCSLPVAYALHGQTISSGSVYVAPPDNHLTLVAGSMRVVRGPKENG
                     HRPAVDALFRTASMAYGSRVIGVVLTGERDCGTAGMLSIKARGGLAVVQEPADAAAPS
                     MPNSAIQNCAVDHVLPLDRVAELLARLALEPAGPAPELTSAVRQLEGTEDAPGAVMVC
                     PHCHGTLQQIDSGQFLGFRCHVGHAFSLQSLASEQAEEVERALWSALRALEESVQMTR
                     RFGLHSTGELKRRFEQKAAEHAQQAETLRRMLLGGVPSVVEDSPSP"
     gene            complement(1446..2099)
                     /locus_tag="VFQ61_29635"
     CDS             complement(1446..2099)
                     /locus_tag="VFQ61_29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9958706.1"
                     /translation="MTLRVLLADDHPVVRDGLKVLIESQADMCVVGEASDGAATLDAI
                     PKVQPDLVVMDLSMPNLGGAEATERIRANHPEVKVLGLTAHEDRGYVQLLINAGAAGF
                     VLKRAAADDLIRAIRAVAEGGMYIDPSVAAQILTLRRNSAPIPDGAVQVAQLSHRESE
                     VLRLIAQGIAIKEVAASLNLSVRTIETYKARGMEKLSLKTRADLVRYALQRGWLKDF"
     gene            complement(2161..>4519)
                     /locus_tag="VFQ61_29640"
     CDS             complement(2161..>4519)
                     /locus_tag="VFQ61_29640"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET9958707.1"
                     /translation="RTLEAPPALQIFATDLNEDVILTARNGVYSDSVVADLSLERLRR
                     FFIKEHQGYRVRRELREMLLFAVHDLLKDSPFSRLDMVSCRNLLIYLNRDAQQRAFDI
                     FHFSLRPEGRLFLGASEAIDDNSQAFVPTDKKFRIYMPRSAVRMNLPIPPSAPFSLRA
                     PGGVHEKLEVSLPPSTPDESAGVGIALLRRTRQEHGSWSELHFKLIERFGPPSLLVNN
                     QHEIVHLSENVGRFLQFTGGEPSMSLLRAVHPALRVDLRAALYRANQSGEPTSALRVP
                     MDLGGARLSVDIHVSPAGESATDFLLVVFDAHENTDKDTARTGPQEDEPVVWHIEREL
                     EQTKRHLRDLVEQHEASTEELKASNEELQAMNEELRSATEELETSREELQSINEELTT
                     VNQELKAKVDELGHTNSDLHNLMGATAIATVFLDRELRIMRFTPPAVALFSLIPTDVG
                     RPLADLKHRLDYADLLPDAARVLQQLVPIEREVSEYGGRVFLARLLPYRTTEDRIAGV
                     VLTFVDVTERHLAQEAVRRAHEELERRVQERTAQLDEANAALRREILEHQRAERARQE
                     LQARLIGAQEEERAHFSRELHDEVGQQLTALMLSLKAIESAVQGEAAAQELRSLRSTA
                     ELVAREIHQLATELRPIVLDDLGLARALTSYIEAWATRTGISVDFVFSGFEENRLPSG
                     IETTLYRIVQEAMNNVVKHAHAKNVSVCIERYEDRVVGSVEDDGEGFDVDSLSESVTE
                     SHRIGIAGMRERAAIVAGTLSLESAVGRGTVVRVNIPLPQSSKMP"
BASE COUNT          824 a         1403 c         1365 g          927 t
ORIGIN      
        1 cacgcagctt cgacttcttt aacatgattt agacattgga gcgcgcgacc catgaatgcg
       61 ttgtagggtg atctcatgct ccagtttggg cacgacatca tcacggtggg cgcatcggcg
      121 ggcggcgtgc aggccttaat caccatgctt tccggtctac ccgcggactt gccagccgcg
      181 gttttcatcg tgcagcatct ctccgcagac tatcagagtc acttgcccga actgctgggc
      241 aggaggtgct ccctaccggt ggcctacgcg ctgcacggac agaccatttc ttccggaagc
      301 gtctatgtag cgccaccaga caaccacctg acgttggtgg ccggctcgat gcgtgtcgtg
      361 cgaggaccca aggagaacgg ccatcggccc gctgtggatg cgctatttcg taccgcgtcg
      421 atggcctatg gcagccgcgt cattggcgtg gtgctcaccg gggagcgtga ctgcggaacc
      481 gctggcatgc tttcaatcaa ggcacgcggc ggactggcgg tcgttcagga accagcagat
      541 gcggctgcac cgagtatgcc gaacagcgca attcagaact gcgccgtcga tcacgtactt
      601 ccgctcgacc gcgttgcgga gcttctcgcg cgtctcgccc ttgagcccgc cgggccggca
      661 cccgaactca cgagcgcggt ccgccagctc gaaggcactg aagatgcccc gggcgcggtg
      721 atggtgtgtc cacactgcca cggaactcta cagcagatag actcagggca attccttggc
      781 ttccgttgcc acgtgggcca tgctttttca ctacagagct tggccagcga gcaagcagaa
      841 gaggtggagc gtgcgctttg gagcgccctc cgcgcgttgg aagaaagtgt tcaaatgacc
      901 cgacggtttg ggttacacag taccggcgag ctcaaacggc gcttcgagca gaaggccgcc
      961 gagcacgctc agcaggcgga aacgctgcgt cgcatgttac tgggtggcgt gccgtcggtt
     1021 gtcgaggata gtccatcccc ataagtggct agaatatgac tcatgcggtc gagattcaga
     1081 ggtgaggcgc tctacccaga cgcgaaacta gagcctcggt cgtgttccgc tcccaagcgc
     1141 gggactcggg tacagcgcct cgcctctgaa tctcagccta tctcgtctgt ttagatcaag
     1201 agcaagcagt cgtgcatttt cccgtagctt cagccgtgct ggctacgtgc tcttcttcac
     1261 tcgcgcgctc tgctcgctcc agcgctctgc tcgctccagc gcgctgttct cggcggggca
     1321 ttcgggtcac ttgactgtcc ttctgtcgaa ggagaaggtg ccgagtaaat gctattcgac
     1381 tgcaactagt ctgtccgtaa agcccgcgcg aacgcgtcat gaaatttgcg gacgacccac
     1441 tagggtcaaa aatccttcag ccacccgcgc tgcagcgcat accgaaccaa gtcggcgcga
     1501 gttttcaggc tgagcttttc catgccccgg gccttgtatg tctcgatcgt gcgcacactg
     1561 agattgaggc tcgccgcaac ctctttgatt gcgataccct gcgcgatcag gcgcaatacc
     1621 tcgctctcgc ggtggctcaa ttgggcaacc tgcactgcgc catccgggat gggagccgag
     1681 ttgcggcgta gggtgaggat ctgtgccgct acgctgggat caatgtacat gcccccctcg
     1741 gcgaccgccc ggatcgcgcg gatcaggtcg tcggcagcgg cgcgcttcag cacgaaccca
     1801 gccgcaccgg cgttgatgag cagctgcaca tagccgcggt cttcgtgcgc ggtcaagccg
     1861 agcaccttca cttcggggtg attggcccga atacgctcgg tcgcttctgc cccgcccagg
     1921 ttgggcatgg acagatccat gaccacgagg tcgggctgaa ctttggggat ggcgtcgagg
     1981 gtcgcggcac catcgctggc ttcgccgacg acgcacatgt ctgcctgcga ctcgatgagg
     2041 actttgagtc catcacgtac gacaggatga tcgtccgcta gtagtaccct caatgtcacg
     2101 tgattcgcgc tcctcgcccg tatgtggacg cccgtcgtcc actgtccggg cttctccggt
     2161 ttacggcatt ttcgagctct gcggtagtgg gatattcacg cgaaccacgg tgccgcggcc
     2221 aactgcggat tcgaggctaa gagttccggc gacgatcgcc gcgcgttctc gcatgccggc
     2281 gattccgatg cggtgcgatt ctgtcacact ctcgctcaga gaatcgacat cgaagccctc
     2341 tccatcgtct tcaaccgagc ccacgacgcg atcttcgtaa cgctcgatgc aaacacttac
     2401 gttcttggcg tgcgcgtgct tcacgacgtt gttcatcgcc tcctgaacga tgcgatagag
     2461 ggtcgtctcg atcccgctcg gcagccggtt ttcctcgaag cccgagaaca cgaagtcgac
     2521 cgatattcca gttcgcgtgg cccaagcctc gatatagctc gtcaaggcac gggccaagcc
     2581 gagatcgtcg agcactatag gccgcagttc cgtcgccaac tggtggatct cgcgcgccac
     2641 cagttcagcc gtgctccgga gcgagcgcag ctcttgtgcc gcagcctcgc cttgcacggc
     2701 cgactcgatg gccttcaaac tgagcatcag cgcggtgagc tgctggccaa cctcgtcgtg
     2761 gagctctcga gaaaagtggg cgcgctcttc ctcctgcgct ccgatcagac gagcttgcag
     2821 ctcttgccgg gcccgctcgg cgcgctgatg ctcgagtatt tcgcgccgca gcgccgcatt
     2881 cgcttcgtcg agctgagccg tgcgctcttg aacgcgccgc tccaattctt catgcgcgcg
     2941 gcgtaccgcc tcttgggcca agtgcctctc cgtaacgtcg acgaacgtga gcaccacgcc
     3001 cgcgatccgg tcttcggtag tgcggtaagg tagcagccta gctaggaata ccctgccccc
     3061 atactccgat acctcgcgct cgatcggaac gagctgttgc agaacccgag ccgcatccgg
     3121 cagcaagtcg gcgtagtcga gccgatgctt caagtcggcc agcggccggc ccacatcggt
     3181 cgggatgagg ctgaataacg caacggcggg cggcgtgaag cgcatgattc ggagttcgcg
     3241 atcgagaaaa acggtcgcga tcgcggtggc gcccatcaaa ttgtgcaggt cgctgttggt
     3301 atgcccgagt tcgtcgacct tggctttgag ctcctggttg acggtggtca gctcttcgtt
     3361 gatggattgt agctcttctc ggctggtctc gagctcttcc gtcgcagacc gcagctcctc
     3421 gttcatggcc tggagctctt cattgctggc cttcagctct tcggtcgaag cctcgtgttg
     3481 ttccacgagg tctcgcaaat gacgtttggt ctgctcgagt tcgcgctcga tatgccaaac
     3541 gacgggctcg tcttcttgcg gaccggttcg cgcggtatcc ttatctgtgt tctcgtgggc
     3601 gtcgaacacc accagaagaa agtccgtcgc gctctcgccc gctggcgaaa cgtggatatc
     3661 cacgctcaag cgagctccgc cgaggtccat cggcactcgc aacgccgatg tcggctcgcc
     3721 gctctgattg gcacggtaca aggcggccct gaggtcgact cgcagcgccg ggtgcactgc
     3781 gcgcagcagg ctcatgctgg gctcaccgcc ggtgaactgc aagaaccgcc ccacgttctc
     3841 ggaaaggtgc acgatctcgt gttggttgtt gaccagcaac gacggtgggc cgaaccgctc
     3901 gattagcttg aaatgcagtt cactccacga gccatgctcc tgtcgggtgc gacgtaaaag
     3961 agcaatcccg acgcctgcgc tctcgtcggg cgttgacggt ggcaaggaga cctcgagctt
     4021 ttcgtgcacc cccccgggtg ctcgcagcga gaagggcgcg gacgggggga tgggcagatt
     4081 catccgtacc gcggaacgcg gcatgtaaat ccgaaacttc ttatcggttg gaacgaacgc
     4141 ctggctattg tcgtcgatcg cctccgaggc cccgaggaac aaacgccctt caggacgtag
     4201 agagaaatga aagatgtcga acgctcgctg ctgagcgtcc cgattcaaat agatgaggag
     4261 gttgcgacag gacaccatgt cgagccgcga aaatggcgag tccttgagca gatcgtgcac
     4321 cgcgaagagc agcatctccc gcagctctcg acgtacccga tagccttggt gctccttgat
     4381 gaagaaccgg cgcaggcgtt cgagcgagag atccgcgacg acagagtcgg agtagacgcc
     4441 gtttcgcgcg gtcaagatca cgtcttcgtt gagatccgtc gcgaaaatct ggagcgcggg
     4501 aggggcctcc aaggttcgc
//
LOCUS       DASTKU010000846        31180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1952922, whole genome shotgun sequence.
ACCESSION   DASTKU010000846 DASTKU010000000
VERSION     DASTKU010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 31180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31180
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952922"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VFQ61_29645"
     CDS             <1..789
                     /locus_tag="VFQ61_29645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET9958708.1"
                     /translation="GTRAAFQTASEKSAAFSFRFENAPRTLASDFLVLDIETVLDPEL
                     PIAASNEADRLPPPPHHKVVVIGALLFDGGYEPKRLGVMSESKDEPGILTDFAKLLEE
                     RRPCLVTFNGRGFDLPVIAMRCLRYGIPFRHYYRSRDVRYRFSADGHLDLMDYVADFG
                     ATRPTRLDVIAKLCGMPGKVGVDGKDVGPLVHAGRIGEVRNYCLCDVVQTAGVFLRVQ
                     LLRGELARENYLTAMGRLLEMIRSDPRLEPVVQALNERRLLLNE"
     gene            861..1223
                     /locus_tag="VFQ61_29650"
     CDS             861..1223
                     /locus_tag="VFQ61_29650"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002019.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET9958709.1"
                     /translation="MQQENTGRPLAQRTCAPCSGSTPRLDASQIALLLPEVPGFELDD
                     EGKSISRDFQFKNFHETMAFVNAAAFIAHREDHHPDLEVGYRHCRVIFRTHANGGLSE
                     NDFVSAAKVNALLSREER"
     gene            1654..2688
                     /gene="purU"
                     /locus_tag="VFQ61_29655"
     CDS             1654..2688
                     /gene="purU"
                     /locus_tag="VFQ61_29655"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HET9958710.1"
                     /translation="MKARSREREEAQLQEMRRASGVIKIERPDPALLSAIAAHADAQG
                     NTNAEAQGEPNLSLRTGPNTATLLVACEPNLDLSYLLSQAFAACGARVIESEQYTHPN
                     SARWFLRMHADTSRMHHGIEALSIALGTISRELGLIFHSEHLNRKKRVALFVSKYDHC
                     LYDLLFRHDAGELDCEIPLIMSNHRDLEPVAKQFGVPFQVVPKTAANKAEAEDLECGL
                     LAMADVDLVVLARYMQIMSREFVTRFSRRVINIHHSFLPAFIGGKPYHQAHERGVKLI
                     GATAHYATANLDEGPIIEQDVVRCTHKDSVEDLVRKGRDLERLVLARAVRLHLEDRVI
                     VEGNKTVIFD"
     gene            complement(3025..4278)
                     /gene="alr"
                     /locus_tag="VFQ61_29660"
     CDS             complement(3025..4278)
                     /gene="alr"
                     /locus_tag="VFQ61_29660"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET9958711.1"
                     /translation="MNSLRPSNEPAADLDEASRVEVPRVFRPQRALPADAVRPTRAEV
                     NLANLRHNLRVAQRAAPGAAIWGVLKADGYGHGSKAVARTLERAGMSGVCVALLEEGI
                     ELREAGIQLPILIMGGYYGRAWAELVRHELTPVLHEESQVRLLADELRHAGVAGFKVH
                     LKIDTGMSRLGVLPDAVEQVGELVRSRPEIALEGLMTHLSNADSGDAASVDRQLQLFS
                     RARATLQSLGLEPRALHAANSAALLGYPSARYSMVRPGIALFGVDPCAPTALAAFPER
                     SGELRPVMRVRTEVVALRKLSPGTPVGYGGSFRASRLSVIATIPMGYADGLSRGLSNV
                     GHVLVRGTRAKIVGAVSMDMTMIDVTDAPGVQLGDECVILGHQKGPCGEDAIGAREIA
                     DQLRSIPWEVLTSISRRVPRFYREP"
     gene            complement(4379..4945)
                     /locus_tag="VFQ61_29665"
     CDS             complement(4379..4945)
                     /locus_tag="VFQ61_29665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958712.1"
                     /translation="MRASLSVSIRALLALSAWLSACGPSVQSIYEGDVRFEHCYRLDL
                     DLEIAPTHREACWSHWLQAYTYGQPRDRIEYARRRVRAFSNGDISRPLLEVSSASKSR
                     QFYLAAPEPTSVHAPPPPIAPKVEVPPPPPSAAPDPIPAPADLCAKECRTKFETCQSV
                     CSGDAAAPEAACKGCPADYKVCMRRCFQ"
     gene            5064..6263
                     /gene="ribB"
                     /locus_tag="VFQ61_29670"
     CDS             5064..6263
                     /gene="ribB"
                     /locus_tag="VFQ61_29670"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HET9958713.1"
                     /translation="MNRSSPDTARPLDISVQELGRVQAALAQIRAGRMVILVDDEDRE
                     NEGDLCMAADCVTPEAINFMCIHGRGLICVTLTEEQVKRLDLPMMTAPGRSGPRLGTA
                     FTVSIEASEGVTTGISAADRALTIRVASHPEAKPNDVVVPGHTFPLVARKGGVLVRAG
                     QTEGSVDLARLAGLNPAGVVCEIINDDGSMARMPDLEAFAQRHDLMILTIADLIAYRL
                     QNEHQVQRLTESEIVLDRTETTWRSVVFETLDKKQILACVKGDVSNDEPVLCRMHQGS
                     TLGDVFSSAATEGRRNLEEAIDRIEEAGRGVIVYLPPRGDLSAELSAREARHEASPPS
                     EGQAAASRPHSNTLREYGLGAQVLRDLGVRRLRLLTSNPKKIAGIQGYGLELVETLTL
                     SSRRGAK"
     gene            6326..6814
                     /gene="ribE"
                     /locus_tag="VFQ61_29675"
     CDS             6326..6814
                     /gene="ribE"
                     /locus_tag="VFQ61_29675"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET9958714.1"
                     /translation="MQAPDLQVIEGQFSVAESARFAIVASRFNSFVVERLVEGALDGL
                     ARHGVNSSRITLVRVPGAWELGLVAARLARSEKFSAVIGLGAVIRGATAHFDYVAGEA
                     ARGLAAASQRIPVVFGVLTTDTIEQAVERAGTKAGNKGFDAALTALEMVSLSEALGAL
                     GY"
     gene            6816..7220
                     /gene="nusB"
                     /locus_tag="VFQ61_29680"
                     /pseudo
     CDS             6816..7220
                     /gene="nusB"
                     /locus_tag="VFQ61_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
     gene            7369..7887
                     /locus_tag="VFQ61_29685"
     CDS             7369..7887
                     /locus_tag="VFQ61_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HET9958715.1"
                     /translation="MDLRFTQPSLRKLDLCGTEVLSCGLASDERPPHGVAGLVDFRLS
                     GRISRLIQSGFATGRVGEVLLVPGKPKLPFDKVLLFGMGPIAGYTEHAYRAVIEKMLE
                     TLSGLRARTAVVELPGRHRDLIAPERAADILLEVAGGRPEHDIWTLVEPGEAQRVITQ
                     HMIQERRRVRQS"
     gene            complement(7931..8419)
                     /locus_tag="VFQ61_29690"
     CDS             complement(7931..8419)
                     /locus_tag="VFQ61_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET9958716.1"
                     /translation="MSAIDKQALLQALRERVQTQLADAKARAKEAAEGATHEENRAES
                     DKDMRSTEASYVARGHAELTGKLEAALNRLSALEVKAFGPNDGIETGALVELALGSKR
                     WLYFLVPAAGGERLPFAGREVLTLTPQSPMGTALLGLGEGDEAEVASPQGSRMYEVVS
                     VR"
     gene            complement(8419..9243)
                     /locus_tag="VFQ61_29695"
     CDS             complement(8419..9243)
                     /locus_tag="VFQ61_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET9958717.1"
                     /translation="MTQRDKVTGLSSKDRVDLLLVERGLAPSRAKAQALVLAGQVFSG
                     EQRIDKPGQTLPRNALLRVQAGPRYVSRGGDKLEGALVDCALSVEGSVCVDVGASTGG
                     FTDCLLQHGAARVYAVDVGEGQLAQRLTRDARVVVMDRTNARHLAPESFPERIDLAVV
                     DASFIGIELLAPALARILPVGSRLLALVKPQFEAGRREATRGRGVIRDPALRAELIER
                     ASHTLLTHGFELLAGHDCRVRGPKGNLEHFLLAKRDARDQKNSPLTLDESAEESAT"
     gene            9361..10128
                     /locus_tag="VFQ61_29700"
     CDS             9361..10128
                     /locus_tag="VFQ61_29700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958718.1"
                     /translation="MMNATLRMMIVSGGLATLALSSTALAQEATVQGQVGMGLPGAAP
                     QTATATRAAGGTDHSLVVGHLGVGYLGRSQIQVGAPGGPGGSAAVAAPIIGVRYWMDP
                     TLGIDVGLGFGFDGGSNTNAGVDTDQPSHWAFLLHGGVPLALAAGRHYTFEIVPEANV
                     GFGGGSATVNGVDISNKGFMLDLGARVGSEIQFGFMGIPELALQASVGALFATSSASS
                     TPDGGGKVSQSQTSFRTTVYNSPWAIFSSNVAALYYF"
     gene            10214..10708
                     /locus_tag="VFQ61_29705"
     CDS             10214..10708
                     /locus_tag="VFQ61_29705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958719.1"
                     /translation="MNFAHSSGSWLAALCWIGVLGLACSGGGANVDSLADTAADPGAT
                     SAPNDTQSAVPPEQTEAPDDSDLPHEPEREAEPPASGCRGLCDRVFALQCEGVDDEVC
                     MQNCNEWPTAPCGPQLQNVVTCLIEANTCPDPDDLAAALPDNCRGRLQAYTTCEDMNQ
                     PTDM"
     gene            10965..13349
                     /locus_tag="VFQ61_29710"
     CDS             10965..13349
                     /locus_tag="VFQ61_29710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET9958720.1"
                     /translation="MWIDPVLLACLAALAGAILPFATSASLVGAACALCLLAPRVHWR
                     LLLVLVGCFSCGAGRAAWQTQQYEAARVSARESLGAPQRCEVTGRVESSPLWSGERLQ
                     YALRVERAECENGTVPDASLVRLGGGPADLARGDRMLAIVDLAPVELWKNPDLSDPTP
                     SAARRGVLLSGATLSAEIVERAHSLTSLVDRARAHVRARILASFAPAVQAMARALVLG
                     ENDLDDGDALAFRKSGLSHMLAVSGTHLVFAVVSIVNALRALLVRVRALSERCDVGRL
                     AHAIGIALALGYADFAGGSGSAERAAWMLSAAFAARALGRPVQTSRSLALSLGVGWLL
                     DPLVSFDLSFLLSAAATVGLLALSKPLAAPVDLVSSKLLRGLGRAMATSLAAMLPCTP
                     LLLLFGDGLTLVGVFANVVAAPLGETLALPLCLTHALCSALPPLEAGLAHAASGALFA
                     VRWIALRSAEQTYLNLPLPAPNAFQIALVLVLGQSLLLGGSEPVWRRRVHLLVGVLGL
                     CSIELAQRAAGSPSGKLRFTALSVGQGDASLLDLPDGKLMLIDGGGAASGGADPGTRV
                     VLPTLAARRRSRIDFVVLSHPHPDHAAGLAAVLANVEVGELWDTAQGRAESAGPSYAA
                     VLRLARERRIPIREPKDLCGRPQAFGALRVRVLAPCPSFTPGRSANDNSFVLRLEFGS
                     HALMFMGDAEHAAEMDLMQSGAPLQADVLKIGHHGSRTSSTAAFLERVHPRVATISCG
                     ARNRFGHPAPEVLERLSALATYTVRTDWDGGARIESDGARLTLERDPLLFTSGN"
     gene            13383..13925
                     /gene="hpt"
                     /locus_tag="VFQ61_29715"
     CDS             13383..13925
                     /gene="hpt"
                     /locus_tag="VFQ61_29715"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET9958721.1"
                     /translation="MSAKPPLTAVKPRELISAQTIADRVTALGRQISEDYAGREPVLI
                     CVLKGSFVFAADLARAIDLPLRLEFFGVQSYGQQTTSSGVVQITLDLKRPIHGEEVLI
                     VEDIVDTGLTLSYLSEQLSARGPKSVKIAALLHKPSRMQRAVNIDYLGFTIDDVFVVG
                     YGLDHAERYRNLPYIGMLEP"
     gene            complement(13953..14345)
                     /locus_tag="VFQ61_29720"
     CDS             complement(13953..14345)
                     /locus_tag="VFQ61_29720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958722.1"
                     /translation="MELKYGGLSLGEYARFCTERERLIEELVGGWSALTEPPPLELLE
                     LCRKYGMNALHPVTGRFHPFIEAWESALGDDPWLLDAFGELATLSDASASELARAESI
                     EREASSERPSHLELVGLSNGSGTYRKVV"
     gene            complement(14503..15636)
                     /locus_tag="VFQ61_29725"
     CDS             complement(14503..15636)
                     /locus_tag="VFQ61_29725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958723.1"
                     /translation="MSTWDTRASSVVLQYRRGLLDGGGFNVISYNANFSATSGKLSSQ
                     FGLHYLSLAETSQTTAHGLAATAAAVFNFPLTDRFDNGLPLASLGLVLGSAPTALVSG
                     ERNFVTVPLVLGLAAPITPAKVITLTPWFELAPSLNLDTIIKPYDLQAEAADWQRYVD
                     INTRTINFTAADVERILSKSVELDTSAAVGARGGLDLALHASDGFDFVANATLSSVGT
                     AFSGARVWYLGGGFAWRWDDIVPAVLPPEKRLQNESCEDIEQRFRSCPNSRRWRSPEE
                     MPTSPTMNPPPLPPVGAPPPSTPPLAPGLDGAPSPATAPPGAAGNEGNSAANPAGATT
                     IPTAPSASAPYNGPTPTSPPPNSSTGTPAEPPPMQSVPTEPIR"
     gene            complement(16163..17740)
                     /locus_tag="VFQ61_29730"
     CDS             complement(16163..17740)
                     /locus_tag="VFQ61_29730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958724.1"
                     /translation="MNDAFKPKLWTPGDFNALFGFGTNILVNLLVLTGLLRFVLKMPD
                     ELVFGRILPATGLMLALSTVYYAWLAYRLAKTSGRTDVCALPSGISVPHMFVVTFVIM
                     LPIASKTGDPVKGWEAGLTWVFVQSFVLMLGGFIAPLIRKVTPRAALLGTLAGVSVAF
                     ISMRPALEMFMNPTIGITCFAIILASWFGGVRYFKGLPAGLVAILVGSAIAWSSAAFG
                     LTDSGMSLAKLSEAVNGFGFSVPVPAFSHVFSGFQFLGVILVTAIPFGIYDLVEAMDN
                     VESAAVAGDSYPTTRVLTADGVVSLIGCLMGNPFINAVYIGHPGWKAMGGRIGYSAAT
                     GAMVLVLSWLGVISLLVTLIPVVAISPILLYIGMLIGAQAFQETPKSHAPAIVLSLVP
                     HLAAWGKLQIDNALGAAGTNAHAVGLDKLGQTGVLYHGLELLGGGSILGGLILGAIGA
                     FVIEREFVRAAAFAAAGAVLTFLGFMHGEAIGFGQTPAVAVSYLAVAGVLVACGKYAP
                     ALASSARSAEPLPEPAE"
     gene            complement(17815..18495)
                     /locus_tag="VFQ61_29735"
     CDS             complement(17815..18495)
                     /locus_tag="VFQ61_29735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET9958725.1"
                     /translation="MATWIDAEPSAISVNLKTVALVLIDMQRDFLQKGGFGESLGNDV
                     SRLSSSIEPCRALLTAARRHGVFVVHTREGHRPDLSDVPRAKLERSPAGRRIGDAGPM
                     GRILVRGEMGHDIIPELYPAPHEPIVDKPGKGAFFATDLHAMLIARGIEVLIVCGVTT
                     EVCVHTTVREANDRGFRCIVPRDACASYFPEFHQAALAMISAQGGIFGWVSDSKRVLS
                     ALSDAPAS"
     gene            complement(18656..19486)
                     /locus_tag="VFQ61_29740"
     CDS             complement(18656..19486)
                     /locus_tag="VFQ61_29740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958726.1"
                     /translation="MKGASSTDELSTSLDAGWDEPSTDSEPVLSSDTALRSDSTLHSE
                     PPSEAEFEALDAGWDEGGGELGRSPVENPTGKARVHDPRSVRQSAPQPNPQRGAPPPK
                     LGKKERRELERRNRAHASQRKRERKEEKRAQRAEPTPASPAQRPSAVAKSRDAAAHEA
                     RAKKPGAKTSSAKKAGKGKSPAQRETSIQSVSHSSSASALESKATSRNEAKAVVSSPE
                     SSTTSRVGAPARGAIAGPERGEARSRQREQPTAKRVPGLLWAVLIGVLALIGLVLFRH
                     "
     gene            complement(19486..20133)
                     /locus_tag="VFQ61_29745"
     CDS             complement(19486..20133)
                     /locus_tag="VFQ61_29745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958727.1"
                     /translation="MVWSRQVQSMGAWPLGAMFVPARFTAARFIATMVAAGALATGCG
                     PAPMLRMTDGSNYRSFVQDRSGGAPTEDLSRLRFRPELCQGEDLRPETATLDELHLVR
                     FLEKQGIDVRVERPRADLTYLLISGQGTQTPIRVRVAILTDADAAGRELHEAMLQHGP
                     GSWGVHRSNLAVLGPIGEPADNMTFAAKTKLACWGVFSVTGTDDAFTVPGAYREL"
     gene            20326..21066
                     /locus_tag="VFQ61_29750"
     CDS             20326..21066
                     /locus_tag="VFQ61_29750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958728.1"
                     /translation="MARKNSTSTSMAERPNPPQREDRQLYRALRRLSALARTRFVSSL
                     LLASSLSACGGSVPESATKNRVFYDWTTATSSAPAEFEQHYPPLDFAGAPPVPEYMGV
                     TVLRGGIHLSRPKGWLLRDASNQPGQAYVQYISPSAYSFAIYERPESPEELWRDVLRR
                     YEDDAQAAGARVSGKRVPVGVGLGQGRAYTVERNVEATKRPFLSRSREYVLRGSGRVV
                     LVQIVHDSENLSAVEHELLRVIDTLEVL"
     gene            21063..22019
                     /locus_tag="VFQ61_29755"
     CDS             21063..22019
                     /locus_tag="VFQ61_29755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958729.1"
                     /translation="MKSLFLLGVVCLTACAGAVESSKLEDKARRTVPVPICLKPLERH
                     GAAGVVSSLKPDDYWTLVIPAFDTSSSTVDAGALDCGGRAVFTEKDLLEADGPRTGSI
                     PVKLEDATISPGPDGFKVVWLRTHRFTDSTAGGPLALMRPREGYAEVYATGFYRGGVQ
                     TTRFNIERLGPKLLVTATNEGCAGVKPNQNCESAYSIYLMRAGRLLRAADFPLDRIEY
                     GTAAGVSGLAQYRLTATPVFAEKNIRVVEQVVVKDPNQGAVRKSDLERIFTLDPTGKL
                     VASADSLWTQIMTTRGGTSVPAPGANPPVNPPPPPGGVPPRR"
     gene            complement(22169..22843)
                     /locus_tag="VFQ61_29760"
     CDS             complement(22169..22843)
                     /locus_tag="VFQ61_29760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958730.1"
                     /translation="MSRDACSGRTVGVKLSALLGALPVLFAANLAQAQSAPPERSHGW
                     DRATNIMALSAAGLQLVMPRVFYSDPEVTVGWKARWHLSVLAPSMTLATLTLLNEQVL
                     KESFDSPSPDCEEDVPCDSYGMLSSQSFLAFSALGQGVGVFLVDTTKWSGGRFNVGSF
                     FGNVAVPAVLATITGVGRSAGNWESGGQVWGSAGIGLGLGFGIGLLYATLQRPECGYT
                     GNLICW"
     gene            complement(22897..24471)
                     /locus_tag="VFQ61_29765"
     CDS             complement(22897..24471)
                     /locus_tag="VFQ61_29765"
                     /inference="COORDINATES: protein motif:HMM:NF042526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein"
                     /protein_id="HET9958731.1"
                     /translation="MSTGFVLRAFRIDNSPIDVSRNRFRSLPIRIGRNALNDFTLPHG
                     LVSSFHARIEELDGKLCVRDLGSKNGVYVHLPGAPIPTRIQPQTPVDLAPSNFGFFLG
                     PQFHVQIEFVPLEDDFDRSSPVSGSVLGNRAMLMGASDPGIAPGPLPNAPAAQGNVGP
                     AFADPNFPAAPPPGQPMPGYGMPGAGMGSAAPPPLGPSSPPALGPGLAPGMGPGGMGP
                     GGMGPGGMGQMGPGGMGPGAGGMGQMGPGGMGAPGALPPLPGGNLGGSAPPLANLPGG
                     NFPGGNNNYGYPNPPGGAYPQANAIPEAYGPPMSVGREAKHTGFFGNMGMEYLAFQGL
                     QELASSLMPGTALATTGDVARFITKLHDTVEVFCRCFIPLREGYSQFVSSLDLQRVAM
                     QRSFRRSRAYMAVESARTPEEVVGALLNWKDRSLDAPKAIEGIFADLMLHQVALLDGV
                     MQGVRAMLDELSPQAIESEQGKRIFGQFKALCQAYQRHYEEVSDEERAFARIFGPEFT
                     AAYRQYRQKRAEDENS"
     gene            complement(24633..25592)
                     /gene="tagF"
                     /locus_tag="VFQ61_29770"
     CDS             complement(24633..25592)
                     /gene="tagF"
                     /locus_tag="VFQ61_29770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HET9958732.1"
                     /translation="MSALGVSALGLIGKVPSQGDYVRIRAQRPEFMQLEEWLSENVDF
                     ALSRARPQWDYAVQHGGIQAFVLRPPSTPANVALAGAIAPSGDRAGRRFPVVAAASLA
                     LSTELAASAHLLPLLLEEAWQASSSLVIESTNPDADVHALLEGNPAQSWIELAEVSGD
                     YLAWSQELRLSELWALVHPTPTRDALSYTLRFLGEAIRPWLGKDQAKTPLTLRLPLGQ
                     AGGAALCFWLDLVRRLARWQHKVPSYFWSHDGQAGTLLLHLGEPPKSTLAELWAPSSE
                     RDEFCDLQGVGPAQHLPPLAPAFENVLHDQATVADLLAVAASR"
     gene            complement(25589..29143)
                     /gene="tssM"
                     /locus_tag="VFQ61_29775"
     CDS             complement(25589..29143)
                     /gene="tssM"
                     /locus_tag="VFQ61_29775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HET9958733.1"
                     /translation="MLWVTLICLVLAAASWAVVLIFTWPIWIAIVASVFFVLVVVGVF
                     VFRRVRALMRASALERELLKQAAQQADKARPDRRPEILALQQQMKTAIEALKRSRLGK
                     RGGRSALYALPWYVIIGPPAAGKTTALTQSGLGFITPNGASAGKVRGTAGTRNCDWWF
                     SDQAILLDTAGRLATQEDDRDEWLSFLTTVSKFRAERPLDGMVVALSVEDLLNLSEVQ
                     LEEQAQTLRARIDEVTKHLEMVLPIYVMFTKVDLLGGFTEFFQDLSKAQRAQAWGTSF
                     SLEDDRLEEPARAIETEFELLVRVLHARMLDRLSREPLPEVRSKILSFPMEFQSLKAN
                     LARFVEELCRPNFHNDAPLLRGFYFSSGTQTGRSFDRLLDNISRGLNLQVAGNPAAQR
                     QGPPQSYFVTELFQKVVFPDRHLAMRSESRVRRRFRRQVMLAGGFVFGTLAVITPPAL
                     SYVRNAELIRLTDAAVKEAVALERSPNATATATASVLDLLVDRVQILERASEQAQLYA
                     FWGPYKAPELLVAVKKIYLERLRSMVAGTVQKQLVADVRRVADLNRMDADNFQESYDN
                     VKLYIMLCDLERLQPEWATEHLSIVWARALRSDSDADKDKLLAHSAYYIKALQADPSW
                     AFRKELTIVSRAQGQLSKMPIDELRYSWLQLAARGVPAIRPDQIFRAASLRYWTAPPN
                     AEVPGLYTLLGWQKVKPLLEAKDGRLVIEPWVLGLSGPIQMDEEAQSSATRLRELYFQ
                     RYEQAWRTFLVGLEVKRPQNNQEALTELGALTETNGPYDSLFRVLAQNVRLDATPSGL
                     VDKLIKKGVDAVNSATKPLTGEDAAAVPEREITPVERAFMPMLQFAAMDAPPAAAGAA
                     GGAAGPSKLQQYITELSKLELALRRSIEDPEAQIDADIKGTESTVRALLGRLDLGMRA
                     IVEPWLMNPIYGSRILVVTQGGTYESDKWKTEVWEVYNQKIAGKYPFADSPTEVSLAE
                     FADFFRPQAGILWKYFSTNLEKVFERRGNSFQQKNLRDPLPYRADFMSCLNVSQEITD
                     AIFGTGTEPALSFQVRIHPVGANIAEIQLNVDGTPTTYRNEPERFVPVIWPGKGNPKG
                     GTLLVKGADFQDQIPRLGDFGLFRLLAAGNIKALSGAESGLLTSSFLLTRTGESPVVI
                     DIKPSKSVHPFSPGFFKRLRCPPVTMSAVAPAGGGGGG"
     gene            complement(29168..29845)
                     /gene="icmH"
                     /locus_tag="VFQ61_29780"
     CDS             complement(29168..29845)
                     /gene="icmH"
                     /locus_tag="VFQ61_29780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HET9958734.1"
                     /translation="MNEPVYWACGDILVLAAQLPLSQNLPPPAELRQRMLTALDAMVG
                     RGRAVGVPDPDLAEARYALTAFIDEQILKSNWPGKSEWMQQPLQLLLYREFTAGENFF
                     VRLRALLQQGNRGIALEAYYLCLLLGFRGAYGVSGDMQSLQGFVEAARQQIQRGLPSA
                     NKFGPHVEPKDRAQAARGSGLTILILAVACVFSSALLLFLLERLLAGSARDVLEAMPL
                     GSARYAP"
     gene            complement(29860..>31180)
                     /gene="tssK"
                     /locus_tag="VFQ61_29785"
     CDS             complement(29860..>31180)
                     /gene="tssK"
                     /locus_tag="VFQ61_29785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HET9958735.1"
                     /translation="VSQHHFQQQDRYHEALLRDRLQGVAHYDFGVTELEIDERAFSAG
                     QFRVKKFSAIWPDGASVSCGDGTPEPPPPPRTLPGDAVRIEVLLGLSQETDTSGLVVG
                     DSESSVARRFTREIRNVVDTNTGGSPQEVEWARPNLRVFFGNERREGYTVIRVAELLR
                     QENGQFQVLDTRLPPVLHLRAAPFLANGIERVLANIVARAQQLSGERRQRQAGGNVDF
                     HASETRKFWLLHTLNGSIPRLTHLLATPRVHPEEAYVTLAELAGMLCSFSTGIEPTDI
                     PKFNYLELGEVFEALFAIVLRLLPGGIEQPYVEIPLEHRPDGMFIGRIPDPKLVADEF
                     FVAVKSNMADALVRERVPAVLKMAGWNQIFDVVKQARHGVRVEIEWNPVGALPMKPGM
                     CLFRVRRENSYWDEIAKSQTVALYLPVDSDWSNTTLSLYAIEPSKLR"
BASE COUNT         5659 a        10202 c         9918 g         5401 t
ORIGIN      
        1 ggcactaggg ccgctttcca gactgcctca gaaaaatcag cggcattctc tttccgcttc
       61 gagaatgctc cgcgcacctt ggctagtgac ttcctggttc tggacatcga gacggtgctc
      121 gaccccgagc tcccgatcgc cgcgtcgaac gaagcggacc gcctgcctcc tccgccgcac
      181 cacaaggtag tcgtcatcgg ggcactgctc ttcgacgggg gctatgagcc gaagcgcctc
      241 ggtgtgatga gcgagtccaa agacgagccc ggcatcctga ccgacttcgc caagctgctg
      301 gaggagcgcc gcccctgttt ggtgaccttc aatggtcgtg gtttcgacct gcccgtgatc
      361 gcgatgcgct gtctgcgcta cgggattccg ttccgccact actaccgatc tagagacgtg
      421 cgttatcgct tcagcgcgga tgggcacctc gacctaatgg actacgtcgc cgatttcgga
      481 gcgaccagac cgacgcgcct cgatgtgatc gcgaagcttt gtggcatgcc gggcaaggtc
      541 ggcgtggatg gcaaagacgt cggacccctg gttcacgcgg gtcgcatcgg cgaagttcgc
      601 aactactgcc tgtgtgacgt ggtgcagacg gcgggcgtat tcttgcgtgt tcagctgctg
      661 cgcggcgaac tggcccggga gaactatttg acggcaatgg gccgcctcct cgagatgatc
      721 cgaagtgatc cgcggctcga acccgtggtt caggcgttga atgagcgacg cctattgctg
      781 aacgagtgac tcgagtctgg tattcactga ctgaaacgac gcgggctcgg gctcgcgctt
      841 ctcgagagga gtggattgtc atgcaacagg agaacacagg tcgtccgctc gcgcagcgca
      901 cctgcgcgcc ttgctccggc tcgacgccgc ggctcgatgc ttcccaaatc gccctactct
      961 tgcccgaagt gccgggcttc gagctcgacg atgaaggcaa gagcatctcg cgcgatttcc
     1021 aattcaagaa cttccacgag accatggcct tcgtaaacgc cgccgcgttc atcgcacatc
     1081 gtgaggatca ccatcccgat ctggaggttg gctatcgtca ctgtcgcgta attttccgta
     1141 cgcacgccaa tggtggtctc agcgagaatg acttcgtgag cgcagcgaaa gtgaacgcgc
     1201 tgctcagccg cgaggagcgc tgacaatagg aagcagctcc gctcgccgta catcgcgggc
     1261 aacgcagatt gcagggcact cacccacggc acggagttcc aaaccgcgga gccatcccaa
     1321 ccgtctcgcc gtaagtagat gatctacggt cgctcatcta cttgggtgac cccgctaagt
     1381 tgctgaactc gacttcaacc tgcgcggtga aacacgaaat cgattttttc ggttaaggcc
     1441 cctgcgcgcg agccgttaga tccgtaacgt gcagacctcg cggtgtgaac acggtgcgga
     1501 gcgggaggag aacgccgctc gccgtgggca ccgcggggga cttttggcga gctgaggcgc
     1561 gaggctcgct gaatgtcagc aacttggcgc tgtgaggggc ccgggagggc tcgtcgggcg
     1621 tatttacgcg ctctttttgg aaggggtcca ggtatgaaag caaggtctcg agaacgagaa
     1681 gaagctcagc tgcaagagat gcgacgcgct tcgggcgtaa tcaagatcga gcgtcccgac
     1741 cccgcgttgc tgtcggccat cgcagcgcac gccgacgctc agggcaacac caacgcagaa
     1801 gcgcagggcg agccgaacct cagcctacgc acgggaccga acaccgccac gctattggtc
     1861 gcatgcgagc ccaacctcga tctctcctat ttactgtccc aggcctttgc agcctgcgga
     1921 gcacgcgtga tcgagtccga gcaatatacg catccgaaca gcgcgcgctg gttcctacgc
     1981 atgcacgcag acacctcccg catgcaccat gggatcgaag cgctgagcat cgcgctcggc
     2041 acgatcagcc gtgagttggg cttgatcttc cacagcgagc acttgaaccg caagaagcgc
     2101 gtggccttgt tcgtatcgaa gtacgaccac tgtttgtacg acctgttgtt caggcacgac
     2161 gccggggagc tcgactgcga gatcccgttg atcatgagca accaccgcga tctcgagccg
     2221 gtcgccaagc aattcggggt accgtttcaa gtcgttccca agacggcggc gaacaaggcc
     2281 gaagccgaag atttggagtg cggcctactt gccatggccg acgtcgatct cgtcgtgctt
     2341 gcgcgttaca tgcagatcat gagccgcgag ttcgtcactc gcttctcccg gcgcgtcatc
     2401 aacatccacc attcgttctt gcctgccttc attggcggca agccatatca ccaggcccac
     2461 gaacgcggcg tgaagctgat tggcgccacc gcacactacg cgaccgccaa cctcgacgaa
     2521 ggacccatca tcgaacagga cgtcgtgcgc tgcacccaca aagacagcgt agaggacctc
     2581 gtccgcaaag gccgtgacct cgagcgcctc gtcctagcgc gcgccgtccg cctccatctc
     2641 gaagaccgcg tcatcgtcga aggcaacaaa accgtcatct tcgactgaat cgccacccca
     2701 ccgcgtccgc gatcactccc ggcgtcatca ccaagtcgcc agcgctgtgg tcacgcgcgc
     2761 agcgcgcagc gccttgggtg gggggacccg acaggattca ctcccaagac ttatcacccc
     2821 gtcgcaagcg gggctggggc cccgcgcgca gcgtttcgac ttcggagaga gccccgcccg
     2881 gattccctcc caagagtcat caccccgtcg caagcggggc tggggccccg cgcgcagcgc
     2941 aaagcgcctt gggtgggggg acccgacagg attcactcct ggcgggggga gggtctggaa
     3001 cagaccctcc caaaaaacac ggcctcacgg ctcgcgataa aaccgcggca cgcgccgcga
     3061 aatcgacgtc agcacctccc acggaatgga gcgcagctga tcggcaatct cgcgcgcacc
     3121 gatcgcgtct tcgccgcacg gacccttctg gtggccgaga atcacgcatt catctccgag
     3181 ctgcacgccc ggagcatcgg tgacgtcaat catcgtcata tccatgctga ccgcacccac
     3241 gattttggcg cgggtaccgc gtactaagac gtgaccgacg ttggacaagc cacgactcaa
     3301 gccgtctgcg taacccatgg gtattgtggc gatgacgctg agacgtgacg cgcggaagga
     3361 gccaccgtaa ccaacggggg ttcccggcga cagctttcgt agcgcgacga cctcggtgcg
     3421 gactctcatc acgggcctga gttcgccgct ccgctcgggg aaggcggcga gcgccgtggg
     3481 cgcgcacggg tcgacgccga atagcgcaat gcccgggcga accatcgagt agcgcgccga
     3541 gggatagccg agcagcgcgg cggagttcgc agcgtgcagc gcgcgcggct cgagccccag
     3601 tgactgcaag gtggcgcgag ctcgactgaa gagctggagc tgacggtcga cggacgctgc
     3661 atccccggag tcggcattgg agaggtgcgt catcagccct tcgagcgcga tctctggccg
     3721 gctccgcacg agctcgccca cctgttcgac ggcgtcgggt aacacgccca agcgcgacat
     3781 gccggtgtcg atcttgagat gcaccttgaa gccggcaacg cccgcgtgac ggagctcgtc
     3841 ggcgagcaga cgtacctgcg attcctcatg gagtactggg gtgagctcgt gacgcacgag
     3901 ttccgcccaa gcgcggccgt agtagccgcc cataatcaag atcggcagct ggatgccggc
     3961 ttcgcgcagc tcgatacctt cttcgagcaa cgcaacgcaa acgccgctca tgccggcccg
     4021 ttccaacgtc cgcgccaccg cctttgaacc atgaccatag ccgtcggcct tgagcactcc
     4081 ccagatcgca gctcccggcg ccgcgcgctg ggcgacgcgc aggttgtgtc gcaggttcgc
     4141 tagattcacc tccgcccggg tcggacgcac ggcatccgcc gggagagcgc gctgggggcg
     4201 aaacacgcgc ggcacctcca cgcgcgaagc ttcgtccaga tccgcggcgg gctcattcga
     4261 ggggcgcaag gagttcatcg tttcctcgca gggtgtgagc gagcccgccc gtggtcaact
     4321 ttctaactga ttactgaggc cgctctggct ccgatgcaca gccgctggca ataacccatc
     4381 actgaaagca gcgacgcatg cagactttat agtcggcagg gcaacctttg caggcggcct
     4441 cgggagccgc ggcgtcgcct gaacagacgc tctggcaggt ctcgaacttg gtgcggcact
     4501 ctttggcgca caggtcggca ggcgccggga tgggatccgg cgccgcgctc ggaggcggcg
     4561 gcggtacttc gaccttggga gcgatcggag gtggcggcgc gtgaacgctc gtgggctccg
     4621 gcgccgccag gtaaaactga cgcgacttgc tggcggagct cacctccagg agcggccgcg
     4681 agatgtctcc gttcgaaaaa gcacggacgc gccgacgcgc gtactcgata cgatcgcgcg
     4741 gctgaccata cgtataggcc tgcaaccaat gcgaccagca ggcttcgcgg tgagtcggcg
     4801 cgatttccaa gtccaaatcc aggcggtaac agtgctcgaa acgcacgtcg ccttcgtaaa
     4861 tggattgcac gctcgggccg cacgccgaca gccatgcgga caacgccaac agcgcacgga
     4921 tcgatacact taggcttgcg cgcacctggt catcatgccg agcctggcct acagccgcaa
     4981 ccccgatccc cctcgaaatc ccgcgtgtag atccccaccc cggatcccgg gagccagatt
     5041 gtcgtgctat ggctcccggc cccatgaacc gaagcagtcc cgacacggcg cggcccttgg
     5101 atatcagcgt gcaggagctc ggccgtgttc aggcggcctt ggcgcaaatt cgcgcaggcc
     5161 gcatggtgat cctggtcgac gacgaagacc gcgagaacga gggcgacctg tgcatggccg
     5221 ccgattgcgt cacgcccgag gccatcaact tcatgtgcat ccacgggcgc ggcttgatct
     5281 gcgtcacgtt gacggaagag caggtcaagc gcctcgattt gcccatgatg accgcgcccg
     5341 ggcgcagcgg gccccggctc ggcacggcat ttacggtgag catcgaagcc agcgaaggcg
     5401 tcaccacggg catcagcgcc gccgatcgcg cgctcacgat tcgggtggcg agccaccccg
     5461 aagccaagcc caatgatgtc gtggtaccgg gccacacctt cccgctcgta gcgcgaaaag
     5521 gtggcgtgtt ggtacgcgcc ggccagaccg aaggctccgt ggatttggcg cgcctcgctg
     5581 gactgaaccc cgcgggcgta gtgtgtgaga tcatcaatga cgacggcagc atggcgcgca
     5641 tgcccgatct ggaggcgttc gcgcagcgtc acgacttgat gatcctgacg atcgcagacc
     5701 tgatcgcgta tcggttgcag aacgagcacc aggtgcagcg gctcaccgag tccgaaatcg
     5761 tgctcgatcg cacggagacg acgtggcgca gcgtggtatt cgagacgctc gacaagaagc
     5821 aaatcttggc ctgcgtcaaa ggcgacgtga gcaacgacga accggtgctg tgccgcatgc
     5881 accaaggctc gaccttgggt gacgtcttca gctcggcggc cacggagggc cgccgcaacc
     5941 tagaggaagc gatcgatcgc atcgaggagg ccggccgcgg tgtgatcgtg tacttgcccc
     6001 cgcgcggaga cctgagtgca gagctctcgg cacgcgaagc acgtcacgaa gcttcgcctc
     6061 cgagcgaggg tcaagccgcg gcttcacgac cacacagcaa cacgctgcgc gagtacggct
     6121 tgggagccca ggtgctccgc gatctggggg tacgccggct tcggctgctc acgagcaacc
     6181 ccaaaaagat cgctggaatt caagggtacg gcctagagct cgtcgaaaca ctgacgcttt
     6241 cgagccggcg cggcgcaaag tagcgtgcag ccccggcgcc gcatggtaca cgtcggcccc
     6301 tccaagcctc cgactcggac tgaacatgca agcccctgat ctgcaagtca tcgaaggtca
     6361 gttctccgtc gcggagagcg ctcgctttgc catcgtggcc tcccgcttca actccttcgt
     6421 ggtggagcgt ttggtagagg gggccctcga cggtttggct cgtcatggcg tgaactcgtc
     6481 acgcatcacg ctggtacgcg tcccgggtgc atgggagctc gggctcgtcg ccgctcggct
     6541 cgcgcgcagc gagaagttct cggcggtgat tggcctcggc gcggtaatcc gcggcgccac
     6601 cgcccacttc gactacgttg ccggggaagc cgcgcgcgga ctcgcggcgg caagccaacg
     6661 catcccggtg gtgtttggcg tgctcacgac ggacaccatc gagcaagcgg tggagcgcgc
     6721 cggtaccaaa gctggaaaca agggcttcga cgcggccctg acggcgctcg aaatggtttc
     6781 gctctccgag gcgctcggcg cgttggggta ctgagatggg cgcgagatcc acggcacgcg
     6841 aggccgtgct gcaaatgctg tttgcggtcg agatttcgag cgctcctccg gagcaggtga
     6901 tccgtgattt ctggcgtgaa acgccaggag atccggaagg gcgggcctac gccgacgatt
     6961 tgttccggag catcgtgcgc gatctggagg cgaccgacgc gcaaatccgc aacgcgagca
     7021 agaattggcg gctcgaacgc atgacgcgcg tcgatcgcaa cgtgctgcgc gtgggcgctt
     7081 acgagctcgt gcacatgcgt gacgtgccgc gcgccgtgat tttggacgaa gcggtggaga
     7141 tcgcgaagcg cttcggcacc gaagagagcg gcgcattcgt gaacggggtg ctggatcgga
     7201 tcgcagacga ctgcggccgc acggcgaccg atgcggatcg gctgcgcgcg gcggccctgc
     7261 ggggagccgg cacgactgca ccgagcccgc cctcacgccc gccggaatcg acaaccccgg
     7321 ccgcctcgcc ttcggacgcc gaagacacgg agcaggagcc ggactgacgt ggatctgcgc
     7381 ttcacacagc cttcgctgcg caagctggat ctgtgtggga cggaggtttt gtcctgtggg
     7441 ctcgcgagcg acgagcggcc gccgcacggt gtggcggggc tcgtggactt tcgcttgtcc
     7501 ggtcgcatct cgcgactgat tcagagcggc ttcgcgacgg gacgagtggg ggaagtactg
     7561 ctggtacccg gcaagcccaa gctgccgttc gacaaggtgc tgctgttcgg catgggtccg
     7621 atcgccggtt acaccgagca cgcctatcga gcggtgatcg agaagatgct ggagacgctc
     7681 tcggggctgc gcgcacgcac ggcggtggtg gagctgccgg gacgtcatcg tgatctcatc
     7741 gctcccgagc gcgccgcgga cattttgctc gaagtggccg gcggccgccc cgagcacgac
     7801 atctggacgc tcgtggagcc cggtgaggcc cagcgcgtga tcactcagca catgatccaa
     7861 gagcgtcgcc gcgtacggca gagctaacgc gatcagccgc ctgctaccga gcgggggccc
     7921 ggcgtccgcg tcagcgcacg ctcacgactt cgtacatgcg cgatccctgg ggtgacgcta
     7981 cttcggcttc gtcgccttcg cccagtccga gcagcgccgt gcccattggg ctctgcggtg
     8041 tcagcgtgag cacctcacgg ccggcgaagg gcaagcgttc gcccccggcc gcaggcacca
     8101 gaaagtagag ccaacgtttg ctgcccaacg cgagctcgac caacgccccc gtctcgatcc
     8161 cgtcgttcgg accgaaagcc ttcacctcga gcgcgctcag ccggttcaat gccgcttcga
     8221 gcttacccgt gagttcggcg tgccccctcg ccacgtacga agcctcggtg ctgcgcatgt
     8281 ctttgtcgct ttcggcgcgg ttctcttcgt gggtcgcgcc ctcggcggcc tctttggcgc
     8341 gtgccttggc gtccgccagt tgggtttgca cccgctcgcg cagggcctgt agcaacgctt
     8401 gtttgtcgat ggctgacatc acgtggcgct ctcttccgct gactcgtcca atgtgagcgg
     8461 gctgtttttt tgatcgcgag cgtcgcgctt ggccaacaag aaatgctcca agttgccctt
     8521 cggcccgcgt actcgacaat cgtggcccgc gagcagctcg aagccatggg tcaggagcgt
     8581 gtggctcgcg cgctcgatca actcggcacg caaagcgggg tcgcggatga ccccgcgtcc
     8641 gcgcgtcgct tcgcggcgtc cagcttcgaa ttgcggcttc accagcgcga gcagtcgtga
     8701 gccaaccggt aaaatgcgcg caagagcggg cgcgagcagc tcgatcccga tgaacgacgc
     8761 atccaccacg gccaaatcga tgcgctcggg gaagctctcg ggcgcgaggt gacgtgcgtt
     8821 cgtgcgatcc atgaccacga cgcgcgcatc tcgagtcagc cgctgcgcga gttgcccttc
     8881 acccacgtcc actgcgtaaa cgcgcgcagc cccgtgctgc agtaagcaat cggtgaagcc
     8941 acccgtcgaa gcgcccacgt cgacgcaaac gctgccttcc acacttagcg cgcagtccac
     9001 taacgcccct tcgagcttgt cccctccccg cgaaacgtag cgtggtccgg cctgcactcg
     9061 gagcagcgcg ttccgcggca gagtctggcc cggcttgtca atgcgttgct ccccgctgaa
     9121 cacctgcccg gccagcacca acgcctgagc cttggctcgc gatggggcca agccccgctc
     9181 gacgagtagc agatcgaccc gatctttgga cgacagtccg gtcaccttgt cgcgttgcgt
     9241 cataggaaga ttgacgagca gggcccgact gaagcagcat gcccgggctc gcgcgtggga
     9301 gggagcccta accaagaaga acggctccgc gcgacagcca cctcaatgtt ggagaattcc
     9361 atgatgaacg ccacgctcag gatgatgatc gtttcggggg gcttggctac gctggccctt
     9421 agcagcacgg ccctagctca agaagcaact gttcagggcc aagtcggcat gggtttgccg
     9481 ggtgctgccc cccaaacggc taccgcgaca cgggccgccg gcggcacgga ccactcgttg
     9541 gtggtcgggc acctgggtgt tgggtacttg ggacgcagtc agattcaggt tggagccccg
     9601 ggcggaccgg gcggaagtgc cgcggtggct gcgccaatca ttggcgttcg ctactggatg
     9661 gatccgacgc tgggcatcga cgtgggcttg ggattcggtt tcgatggcgg ctccaacacg
     9721 aacgccgggg tcgacaccga tcaaccgagc cactgggcat tcctgctgca cggcggcgtt
     9781 ccgctcgcgc tcgccgcggg ccgccactac acgttcgaga tcgtgcctga ggccaatgtt
     9841 ggcttcggcg gcggctccgc gaccgtcaac ggcgtggaca tcagcaacaa gggcttcatg
     9901 ctcgacctcg gcgcgcgcgt aggttcggag attcagttcg ggttcatggg catcccggag
     9961 ctcgcgttgc aagcctcggt cggcgccttg ttcgccacga gcagcgcgag cagcacgccc
    10021 gacggcggtg gcaaggtgag ccagagccag acgtccttcc gcaccaccgt ctacaacagc
    10081 ccgtgggcga tcttctccag caacgttgcg gcgctctatt acttctgaga ggcagagctt
    10141 gaaagccctc tccctccccg ctgctatcct tcgcactgct accaccgaga atcgtcgatt
    10201 cgggagctct cgcatgaact tcgctcactc ctctgggtca tggcttgcag ccctgtgttg
    10261 gattggagtg ctggggctgg cttgcagtgg cggtggtgcg aacgtggact cgctcgccga
    10321 tacggcggcc gatcccggag ccacgtcggc gccgaatgac acccaaagcg ccgtgccccc
    10381 ggagcagact gaggctcccg atgacagcga tctgcctcac gagccagagc gtgaggcgga
    10441 accgccagct tcgggctgtc gcggtctatg cgaccgcgtg ttcgcgctgc agtgcgaagg
    10501 agtagatgac gaggtctgta tgcaaaactg caatgagtgg cccacggcgc cgtgcgggcc
    10561 gcagctgcag aatgtcgtca cctgcttgat cgaggccaac acctgccccg atccggacga
    10621 cctggcggcg gccctgccag acaattgccg aggccgtttg caggcgtaca cgacctgcga
    10681 ggacatgaac caacccacgg atatgtgacg cgcgggagtt cgagtctgta gagcgggcga
    10741 gggcgtcccc gagccctcgc ccgccatgat tttccgacta actcgcttgt gaatcgagtc
    10801 ggcgaggcca cgtcgagcca cgcgctcgag taggttcgga gtaaccgcca gcgccagcgg
    10861 caaacacccg ctcgagcgcc agccggaaac agcggcggcc agctggtgaa gccgctaaaa
    10921 cagcctcatt tgcgctggcc cgcatcgtgt aaccgtgccg cgcggtgtgg atcgatcccg
    10981 tattgttggc ctgcctcgcc gcactcgccg gagcaatcct gccgtttgcg acgtcggcca
    11041 gcctggtagg cgccgcgtgc gccctgtgcc tgctcgcgcc acgcgtgcac tggcgtctgc
    11101 tcctcgtgtt ggtgggctgc ttttcgtgtg gagcggggcg cgcggcctgg caaacgcagc
    11161 agtacgaggc ggcgcgcgtt tcagcacggg aatcgttagg ggctccccaa cgctgtgaag
    11221 taacggggcg cgtggagagc tcaccgctct ggtcggggga gcggctgcaa tatgcgctgc
    11281 gcgtggagcg cgcggagtgc gagaacggaa ccgtccctga cgctagcctg gtgcggcttg
    11341 gcggaggccc tgcagacttg gcccgcggtg accgcatgct ggccatcgtc gatttggcgc
    11401 cggtcgagct ctggaagaac cccgacctga gcgaccccac accgagtgcg gcccggcgcg
    11461 gggttttgct gagcggcgcg acgttgagcg ccgagatcgt ggagcgcgcc cactcgctca
    11521 cgagcctcgt cgatcgagct cgggcgcacg tgcgcgcgcg catccttgcg agctttgcgc
    11581 cagccgtgca agccatggcg cgcgcgctcg tgctcggcga aaatgacctc gacgacggag
    11641 acgctttggc ctttcgcaag agcggcctct cacacatgct ggcggtgagc ggcacgcact
    11701 tggtcttcgc ggtggtcagt atcgtcaacg ctctgcgcgc gctgctcgta cgagtacgcg
    11761 cgctgtcgga gcgctgcgat gtaggacggt tggcgcacgc gatcggcatc gcgctggcgc
    11821 tcggctacgc cgacttcgcc ggtggcagcg gctccgccga gcgcgcagcc tggatgctct
    11881 ccgcggcctt cgccgcacga gccctcggta gacccgtcca aaccagccgg agcttggccc
    11941 tctcgctcgg cgtcggctgg ttgctggatc cgctggtgag cttcgacttg tctttcctgc
    12001 tgtcggccgc agcgaccgtg ggtctgttgg cactctcgaa gccgctggcc gcacccgtcg
    12061 atctcgtttc ctccaagctg ctccgtggcc tagggcgagc gatggcgacc agcctggccg
    12121 ccatgttgcc ctgcacgccg ctgctcctcc tattcggtga cggactcact ctagtgggtg
    12181 tgtttgcgaa cgtcgtggcg gcgccgctcg gggagacgct cgcgctgccg ctgtgcttga
    12241 cgcacgcgct gtgctcggcc ctgcccccgc tcgaggctgg tctcgcgcac gcggcttctg
    12301 gcgcgttgtt cgcggtgcgg tggattgcgc tacgcagcgc ggagcagacc tacctgaatt
    12361 tgccgttgcc cgctccgaac gcgtttcaga tcgcgctcgt gttggtgctg ggtcagagcc
    12421 tcttgctcgg aggcagcgag ccggtttggc ggcgccgagt gcatctgctc gtgggcgtct
    12481 tgggtctgtg ctcgatcgag ctggctcaac gcgccgcggg gagccccagc ggcaagctgc
    12541 gcttcaccgc gctttcagtg ggccaaggag acgcctcact gctcgattta cccgacggca
    12601 agttgatgtt gatcgacgga ggcggcgccg cttcgggcgg cgccgatcct ggcacccgcg
    12661 tcgtgttgcc gacgctcgcg gcgcgacgtc ggagccgcat cgacttcgtc gtgctgagcc
    12721 atccgcatcc cgatcacgcc gcggggctcg cggccgtatt ggcgaacgta gaagtcggag
    12781 agctgtggga taccgctcaa gggcgcgccg agagcgccgg accgagctac gcggccgtgc
    12841 ttcgcttggc gcgagagcga cgcatcccga ttcgagagcc caaagatctg tgcgggaggc
    12901 cacaggcttt cggcgcgctg cgcgtgcgtg ttttggcccc ttgcccaagc ttcactccgg
    12961 ggcgcagtgc caacgacaac tctttcgtat tgcggctgga gttcgggtcc cacgcgctga
    13021 tgttcatggg cgacgccgag cacgccgcag aaatggatct catgcagtcg ggggcgccgc
    13081 tccaagcgga tgttctgaag atcgggcacc acggcagccg cacctcgagc accgcggcgt
    13141 tcttggaacg agtgcacccg cgcgtggcca cgatttcgtg cggagcccgc aaccgcttcg
    13201 gtcaccccgc ccccgaggtt ttggagcgac tctccgctct cgccacatac accgtgcgca
    13261 cggattggga cggcggcgcc cgcatcgaga gtgacggggc gcggctcact ctcgagcgcg
    13321 atcccctgct gttcaccagt gggaattgac atcccgcacc caccgggtgc ttgcttggcg
    13381 tcatgtcggc caaaccacct ctgactgcgg ttaaaccacg tgagttaatc tcggcgcaga
    13441 ccatcgccga tcgagttacc gcgttggggc gtcagatctc ggaagactac gccgggcgtg
    13501 agccggttct gatttgtgta ttgaagggca gcttcgtgtt cgccgcagat ctcgcgcgcg
    13561 ccatcgattt gccgctgcgg ctcgagttct tcggggttca aagctatggt cagcaaacga
    13621 cctcgagtgg tgtcgtgcag atcacgttgg acctcaagcg gcccattcat ggcgaagagg
    13681 tgctgatcgt cgaagacatc gtggacaccg gactcacgct ctcctacttg agcgaacaac
    13741 tctcggcgcg tggcccgaag agcgtgaaga tcgcggccct gctccacaag ccgtcgcgta
    13801 tgcaacgcgc ggtgaacatc gactatctgg gcttcaccat cgacgacgtg ttcgtcgtgg
    13861 gttacgggct cgaccacgcg gagcgatacc gcaacctgcc gtacatcggc atgctcgagc
    13921 cttgaaccgc ccgcggcacc cgcgatgccg gctcagacta ctttgcgata ggtgcccgag
    13981 ccgttcgaca aacccacgag ttcgaggtgg ctcggccgct cggagctcgc ttcccgttcg
    14041 atcgactctg cgcgcgcgag ctcggaggca ctcgcgtccg aaagtgtcgc gagctcacca
    14101 aacgcatcga gcaaccaagg gtcatcaccc agggcgcttt cccaagcttc gatgaagggg
    14161 tgaaagcgcc cggtcacggg gtgcagcgcg ttcatgccgt acttacggca cagctcgagc
    14221 aactcgagcg gaggcggctc ggtcagcgcc gaccaacctc cgacgagctc ttcgatcaag
    14281 cgctcgcgct cggtgcaaaa ccgggcatac tcgcccaggg acagcccgcc atacttaagc
    14341 tccatgttcg agctccatat ctggtcgcag aatgattcgc gccacagcat gacgcacact
    14401 agggtttcgc accatcgggt gccttacgct gcgtagactg agttgctacg agtctcagag
    14461 ccgtcgttcg gggtttctta gggaaccgta gtgttggggt tcctagcgaa tgggctccgt
    14521 cgggacggat tgcatgggag gtggctccgc cggagtaccg gtcgatgagt tcggcggtgg
    14581 gctcgttgga gttggaccat tatagggcgc gctcgcgctc ggtgccgtcg gaatcgtcgt
    14641 cgcgcccgct gggttggccg ctgaatttcc ctcatttccc gccgcgcccg gcggagccgt
    14701 agcaggactt ggtgcgccat ctaacccagg cgcgagcgga ggcgtgctcg gaggcggtgc
    14761 acccacgggc gggagaggcg gcggattcat cgtcggcgac gtaggcattt cttctgggct
    14821 gcgccagcga cgcgaattgg ggcagctgcg gaagcgctgc tcgatgtcct cgcagctctc
    14881 attctgaaga cgcttctccg gaggcagcac tgcaggaacg atgtcgtccc agcgccaagc
    14941 aaagcctcca cccaaatacc aaacgcgggc accactaaac gcagttccca cggaggatag
    15001 cgtcgcgttc gcgacgaagt cgaagccgtc cgatgcatgc agcgccagat cgagacctcc
    15061 gcgagcaccg acggcggcac tggtatccaa ctcgaccgac ttgctcagga tgcgttccac
    15121 gtctgcagcc gtgaagttga tggtgcgcgt gttgatgtcc acgtagcgct gccaatccgc
    15181 agcctcggcc tgcaagtcgt acggcttgat gatggtatcc aggtttaggc tcggagcgag
    15241 ctcgaaccag ggagtgagcg tgatgacctt ggcgggcgta atcggagcgg ccaacccgag
    15301 cactagcgga acggtcacga agttccgttc accgctcacc agcgccgtcg gtgcgctacc
    15361 gagcaccaag cccaagctgg ccaggggcag gccattgtcg aagcgatccg tcagcgggaa
    15421 gttgaagacg gcagccgcgg tagccgccaa gccgtgcgcc gtagtctgag aggtttccgc
    15481 gaggctgagg taatgaagac cgaactgaga actcagcttc ccgctcgtgg ccgaaaagtt
    15541 ggcgttgtag ctgatgacgt tgaagccgcc gccgtcgagc agcccacgcc ggtactgcag
    15601 caccacactc gacgcccgcg tgtcccacgt ggacaccaca agtggatgcg tgcccggctt
    15661 tgccgacgct gcctgcgaga atgtcaggtg agcgagcgtg agcgctgccg ccacgctcag
    15721 acaacccgcc acacgttgcc cacccgggcg acgtgctcga gaacttccga ttacccgagt
    15781 ggctcggctc cgcgttgcgg cggggagaga gttcgattca ccaacgtcgg gccgagaggc
    15841 atctgcgcaa gctggcttca tgattcacga gaggagggct gagcaactat caggccgcac
    15901 aacggctccg cctacgagcc acttaatccc gtgcgggcgc gctatccctc tgcagccgcg
    15961 gcagcctcac ggcgccgtgc gccctcatgc gaagcggtcg gactctatcc tcgcgttctc
    16021 tcgcgttccg cggcgtgagc acctctccat ctcggatcac tgaatcgcgc aaaggccaaa
    16081 gcgctgacgc tgccgcgtac gcagagcttc cacgtaccac ggcagcgtga tttccggacg
    16141 cttagtaaat gcgcgggcgg cgtcactcgg ccggttcggg caacggctcg gccgagcgcg
    16201 ccgatgatgc gagcgcaggc gcgtacttgc cacatgcaac cagcacccca gcaacggcca
    16261 agtagctgac tgctaccgcc ggggtctgac cgaatccgat ggcttcacca tgcatgaacc
    16321 cgaggaaggt cagcacggcg cccgcggctg cgaacgcggc ggctcgcacg aactcgcgct
    16381 cgatcacgaa agcgccaatc gcgccgagaa tcagcccacc caagatcgag ccgccaccga
    16441 gcaactcgag accatgatac agcacgcccg tctgacccag cttgtccagc cccacggcgt
    16501 gagcgttggt ccccgcggcg ccaagggcgt tgtcgatctg cagcttcccc caggccgcga
    16561 ggtgcggcac gagcgagagc acgatggcgg gagcatggct cttgggcgtt tcttgaaaag
    16621 cctgagctcc gatcagcatg ccgatgtaaa gcaagattgg cgagatcgcg acgactggga
    16681 tcaacgtgac gagcagtgag ataacgccca gccaggacaa cacgagcacc atcgcgccgg
    16741 tcgccgccga gtatccgatg cggcccccca tggccttcca accgggatga ccgatgtaca
    16801 cggcgttgat gaaggggtta cccatcaagc agccgatcag gctcaccacg ccatcggccg
    16861 tgagcacgcg tgtcgtgggg taactgtcac ccgcgacggc cgcgctctcg acgttgtcca
    16921 tggcctcgac caaatcgtag atgccgaacg gaatcgccgt gaccaagatc acgccgagga
    16981 actggaagcc cgagaagacg tgcgaaaagg ccggcaccgg caccgagaag ccgaagccgt
    17041 tcaccgcctc ggacagcttg gcgaggctca tgcccgagtc agtcagcccg aacgcagccg
    17101 agctccaagc gatggcgcta ccgaccagga tggccaccaa accggcgggg agccccttga
    17161 agtagcgtac gccgccgaac caactggcca agatgatcgc aaagcaggta atcccgatgg
    17221 ttggattcat gaacatctcg agcgcgggac gcatcgagat gaacgccacc gagacgcccg
    17281 ccagcgtccc cagcagagcg gcgcgcgggg tcactttgcg aatcaacggg gcaatgaagc
    17341 caccgagcat caacacgaag ctctgcacga atacccaggt caaccctgct tcccaaccct
    17401 tcacggggtc cccggtcttg ctcgcgatgg gcagcatgat cacgaacgtg accacgaaca
    17461 tgtgcggcac gctgatgccc gaaggaagcg cgcagacgtc ggtgcgcccg ctggtcttcg
    17521 ccaagcggta cgccagccag gcgtagtaaa cggtgctgag cgccagcatc agccccgtcg
    17581 ccggcaaaat gcgaccgaac accaactcgt ccggcatctt caggacgaag cgcaagagcc
    17641 ccgtcaagac cagcaaattg accagaatat tggtcccgaa tccaaacaag gcgttgaaat
    17701 cgcccggtgt ccacagcttg ggtttgaatg cgtcgttcat ggcgaggcgc tcccgcgtgc
    17761 gtttgcaggt tctgcagttt tcgatttgtt cggaacaggc gtgtcgcgac cggttcagct
    17821 cgccggcgcg tcggaaagcg cgctgagcac gcgcttcgaa tcactcaccc acccgaagat
    17881 gccgccctga gcgctgatca tggcgagtgc ggcctggtga aactccggaa agtacgaggc
    17941 gcaggcgtcg cgcgggacga tgcaacgaaa gccacggtca ttggcctcgc gcacggtcgt
    18001 gtgcacacag acttcggtgg tcaccccgca cacgatcaac acttcgatgc cacgcgcgat
    18061 cagcatggcg tggagatcag tcgcgaagaa cgcgcccttg ccgggtttgt ctacgattgg
    18121 ctcgtgcggc gccggataca gttctgggat gatgtcgtgc cccatctctc cgcgcaccaa
    18181 gatgcgcccc atgggccccg cgtcgccgat gcgtcgtccc gcagggctgc gctcgagctt
    18241 cgcgcgaggc acatcggata aatcgggccg atggccctca cgtgtatgaa ctacgaacac
    18301 tccatggcgg cgagccgccg tgagcaacgc acgacacggt tcgatgctcg agctgagccg
    18361 cgagacatca tttccgaggc tctccccaaa gcctcccttc tgtaagaaat cgcgctgcat
    18421 atcgatcagg accagcgcaa cggtcttgag atttaccgag atcgcgctgg gctcggcatc
    18481 gatccacgtc gccatgatgg gcagcctttc gcgaggagct cacgtccgcc gacgctcgct
    18541 gtaaggcgat tgccacgctc ctagtttcgc gcgattttcc ccagcgtctc acgcctgatt
    18601 tcgcccgtcg ggattcgccg gcggtcgcag cgctagtttc agcgagtcac gaaattcagt
    18661 gtctgaacag cactaacccg atcagcgcca agaccccgat caagacggcc caaagcaagc
    18721 cgggaactcg tttcgccgtg ggctgctcgc gctgtcgaga gcgcgcttcg ccgcgctccg
    18781 ggccagcaat ggcaccccgc gctggagctc caactcgaga cgtggtgctc gattcgggtg
    18841 agctgacgac tgctttagcc tcgttacgcg aggtcgcttt cgactcgagg gcgctggcag
    18901 agctcgaatg cgatacggac tggatcgaag tctcgcgctg agcaggactc ttgcccttgc
    18961 ctgccttctt ggccgaggaa gtcttggcgc caggcttctt tgctcgcgcc tcgtgagcgg
    19021 cggcatcgcg gctttttgcc acggctgatg ggcgttgggc aggggatgct ggcgtcggct
    19081 cggcacgttg ggcgcgcttc tcctctttgc gctcgcgttt gcgctggctg gcatgcgcgc
    19141 ggttgcgacg ttcgagttcg cgtcgttcct ttttgcccag cttgggcggc ggggcgccgc
    19201 gctgcggatt gggttgaggc gcactttgcc gaacagaacg ggggtcgtga actcgagcct
    19261 taccagtcgg gttctcgacc ggagaacgtc caagctcgcc gccaccctcg tcccaacctg
    19321 cgtccaacgc ctcgaactct gcctcggagg gtggctcgga gtgcagcgta gagtcagaac
    19381 ggagcgcggt atcagagctc agcacgggct cggagtcggt cgagggctcg tcccaacctg
    19441 cgtccagcga cgtgctcagc tcgtcagtgc tggaggcccc cttcatcaca gctcgcggta
    19501 cgcgccggga acggtgaagg cgtcatcggt gcccgtcacg ctaaacacgc cccagcaggc
    19561 cagcttggtc ttcgctgcga aggtcatgtt gtccgcgggc tcgccgattg ggccaagcac
    19621 ggccaagttc gatcggtgca cgccccatga acccggcccg tgctgcagca tggcttcgtg
    19681 gagctcgcgc ccggccgcgt ccgcatccgt cagaatcgcg acgcgcacgc ggatcggcgt
    19741 ctgcgtgcct tggccggaaa tcaagagata ggtcagatcg gcgcgaggcc gctccacgcg
    19801 cacgtcgatc ccctgtttct cgaggaaacg gaccaggtga agctcatcca gggtggccgt
    19861 ttctgggcgc aggtcttcgc cctgacaaag ctccggccgg aaccgcaggc gtgaaagatc
    19921 ctccgtcggc gctccgcccg atcggtcctg cacgaaagat cgatagttcg agccgtcggt
    19981 catgcgcagc atcggggctg gaccgcagcc cgtcgcgagc gcgcccgcag caaccatcgt
    20041 cgcgatgaac ctggctgccg taaacctggc cggaacgaac attgcgccga gcggccacgc
    20101 acccatcgat tgcacctgcc ttgaccacac catgacgttt tccttcgctc tgccgcggaa
    20161 gctatagcac ccgagggcta aacccaagcg cggagcagcg atcgccaaga actaaccggg
    20221 ccggcccgac gctccgttgc gagcgcgagc acggattggt agggtgcggc ggtcgtgaag
    20281 cgaccacgcc ggcttcggct ggttttacag aggttgtccc ggggaatggc ccgtaagaac
    20341 agcacatcca ccagtatggc ggagcgtccg aacccgccgc agcgcgagga tcggcagctc
    20401 taccgcgctt tgcggcgatt gagtgcgctc gcgcggacca gattcgtgag cagcctgctg
    20461 ctcgcaagca gcctcagcgc ttgcggcggc agcgtcccag agtccgcgac gaagaatcgc
    20521 gtattctacg actggacgac ggccacgagc agcgctcctg ccgagttcga gcagcactac
    20581 ccaccgctcg atttcgcagg ggctccgccc gtgcccgaat acatgggcgt tacggtgttg
    20641 cgcggcggca tccacctctc gcgccccaag ggttggctgc tccgcgacgc cagcaaccaa
    20701 cccggccagg cgtacgtcca atacatttcg ccgagcgcgt attccttcgc catctatgag
    20761 cggcccgagt cgcccgagga gctatggcga gacgtgctgc gccgttacga ggacgacgca
    20821 caagcagccg gcgcccgagt gtctgggaag cgcgttccgg tcggcgtggg cctcggacaa
    20881 ggccgtgcat acacggtgga gcgcaacgta gaggcgacga agcgcccgtt cttgagccgc
    20941 agtcgtgaat acgtgctgcg gggaagcggg cgcgtggtgc tcgtgcagat cgtgcacgat
    21001 agtgagaact tgtctgccgt cgaacacgag ctcctccgcg tgatcgacac cctcgaggtc
    21061 ctgtgaaaag cctgttcctg ctcggtgttg tttgcctaac ggcctgcgcc ggcgccgtgg
    21121 aatcttcgaa gctcgaggat aaggcccggc gcacggttcc ggtgccgatc tgcctgaagc
    21181 ccctggagcg tcacggcgcg gcaggcgtgg tttcctcgct caagccagac gactactgga
    21241 cactggtcat tcccgccttc gacacgagca gcagcaccgt ggacgccggc gcgctcgatt
    21301 gcgggggccg cgccgtgttt acggaaaaag acttgctcga agccgatggg ccgcgtacgg
    21361 gctcgatccc cgtcaagctc gaagacgcga cgattagccc tggtccggac ggcttcaagg
    21421 tggtgtggct gcgtacgcac cgcttcacgg acagcacggc gggcgggccg ctcgccttga
    21481 tgcgccctcg cgaaggttac gcggaagtgt acgcgaccgg gttttaccgc ggtggcgtgc
    21541 agacgacgcg cttcaatatc gaacgcttgg gcccgaagct gctagtgacc gccacgaacg
    21601 agggctgcgc cggggtcaaa cccaatcaga actgcgagag cgcctattcg atctacctga
    21661 tgcgagccgg gaggctgctg cgcgctgctg attttcccct cgatcggatc gaatacggga
    21721 cggcggcggg agtgagtggg ctcgcgcagt accggctcac ggcgaccccc gtattcgcag
    21781 agaagaacat tcgcgtcgtc gagcaggtgg tcgtcaaaga tccgaatcaa ggtgcggtgc
    21841 gcaaatcgga tctcgagcgc atcttcacgc tggatcccac gggtaagctc gtcgccagtg
    21901 cggactccct gtggacgcaa atcatgacca cgcgaggcgg aaccagtgtt cccgctcccg
    21961 gcgctaaccc gcccgtcaat ccaccgcctc cgccgggcgg cgtgcctcca cgacgctagt
    22021 actcgaatac cgccaacgcg gccctgttca tcagcagcag ggccgttaag ccgcacgacc
    22081 acgcaagcga cggagctcct gcgcgccgtt cacggcgcgc tgcaaccgaa gctcctgcgc
    22141 gccgttcacg gcgcgctgca accgaagctc accagcagat caggtttccg gtgtatccgc
    22201 actcggggcg ctgcagggtc gcatagagca acccgatccc aaaaccgaga ccaaggccaa
    22261 tcccggcgct gccccaaacc tggccaccag actcccagtt acccgcgctt cggccaacac
    22321 cggtaatggt agcgagcaca gcgggtacgg ccacgttgcc gaagaacgag ccgacattga
    22381 agcgcccgcc gctccacttc gtggtgtcca ccaggaatac gccgacgccc tgacccaacg
    22441 ccgagaacgc caagaaagac tgggacgaga gcatcccgta cgagtcgcac ggcacgtctt
    22501 cctcgcagtc cgggctcggg ctgtcgaacg actccttcaa cacctgctcg ttgagcagcg
    22561 tcagcgtggc gagcgtcatg ctgggcgcca gcacggagag atgccagcgg gccttccaac
    22621 ccacggtgac ctcgggatcc gagtagaaca cgcgcggcat cacgagctgc aggcccgccg
    22681 ccgagagcgc catgatgttg gtcgcgcggt cccagccgtg gctgcgttct ggcggcgcgc
    22741 tctgggcttg cgccaaattg gctgcaaaaa gcaccggcag cgcgccgagc agcgctgaaa
    22801 gcttgacccc aacggttcgc ccagaacatg cgtcgcgtga cacgggcgcc attatgctcg
    22861 ggggccgttg gatagcaacc cttgattccc gagagctcac gagttctcgt cctccgctcg
    22921 tttctgacgg tattgccggt atgcggccgt gaattcaggc ccgaagatgc gagcgaacgc
    22981 gcgctcctcg tccgacactt cttcgtaatg ccgctggtac gcttgacaga gcgctttgaa
    23041 ctgaccgaaa atgcgcttgc cctgctcgct ttcgatggct tgtggtgaga gctcgtcgag
    23101 catcgctcgc acgccttgca tcacaccatc cagcagcgcc acttgatgca acatcaagtc
    23161 cgcgaagatg ccctcgatcg ccttgggagc gtccagggag cggtccttcc agttcagcag
    23221 cgcgcccacg acctcttccg gcgtgcgcgc gctctccacc gccatgtacg ctcgcgagcg
    23281 ccggaagctc cgctgcatcg ccacgcgctg cagatccagc gaagagacga actgtgagta
    23341 tccttcgcgg agcgggatga aacaccggca aaacacctcg accgtgtcgt gcagcttggt
    23401 gatgaaacgc gccacgtcac cggtggtcgc gagtgcggtg cccggcatca aggacgacgc
    23461 gagctcttgg agaccctgga aggccaggta ctccatgccc atgttgccga agaacccagt
    23521 gtgctttgcc tcgcgcccga ccgacatcgg tgggccgtat gcctcgggaa tggcattggc
    23581 ttgcggataa gccccgccgg gagggttggg atatccgtag ttgttgttgc cacccgggaa
    23641 gttaccgcct ggtaggttcg cgagtggtgg agcgctcccc ccgaggttgc cacccggcaa
    23701 aggcggcagt gctccgggcg cacccattcc gccgggtccc atctggccca tgcctccggc
    23761 gcccggtccc atcccgcccg gtcccatctg gcccatgcca ccgggtccca tgccgccggg
    23821 tcccatgccg ccgggtccca ttcccggggc taatccgggg cctaaagccg ggggactaga
    23881 cgggccgagc ggtggtggcg ccgcggagcc catgccggcc cccggcattc cgtagccggg
    23941 catcggttgg cccggcggcg gcgcggcagg gaaattcgga tcggcgaaag cgggccccac
    24001 atttccctga gctgcgggag cattggggag tggtccgggc gcaatgcccg gatccgaagc
    24061 gcccatgagc atggcgcgat ttccgagcac gctaccgctc acgggtgagc tgcggtcgaa
    24121 atcgtcctcg agcggaacga actcaatctg tacgtggaat tgcggcccca agaagaagcc
    24181 gaaattcgag ggggctaaat cgaccggcgt ttgcggctga attcgcgtcg ggatgggcgc
    24241 acctggcagg tgcacataga cgccgttctt gctgcccagg tcgcgaacac acaacttgcc
    24301 gtcgagctct tcgatccgtg cgtgaaagct cgaaaccaag ccatgcggca gcgtgaagtc
    24361 gttcagcgcg ttgcggccaa tgcggatcgg cagcgaacgg aagcgattcc gcgaaacgtc
    24421 gatcggcgag ttgtcgatcc tgaaggcgcg cagtacgaaa ccggtcgaca cgggcggata
    24481 ctatcgcgga tcgccgccct gagttctaat tctaagctgc caatcggccg ggctgccgga
    24541 gaccagcacc atcagctcga gggctgacag ccgatcgtaa gcaccactcg aagccgtcat
    24601 gccaggaggg gcccgtcagg ttccgggctt gctcagcggc tggccgcaac agctaacaaa
    24661 tcggcgacag tggcctgatc gtggagcacg ttttcgaacg caggcgcgag cggaggcaag
    24721 tgttgagccg ggcccacgcc ctgcaagtca cagaactcgt cgcgctcgct gctcggagcc
    24781 caaagttccg ccagcgtact cttcggcggt tcacccaaat gcaaaagcaa cgttccggct
    24841 tgcccgtcgt gagaccaaaa atagcttgga accttgtgtt gccagcgcgc taagcggcgc
    24901 accaagtcca accagaaaca aagggcggcg ccgcccgcct gaccgagcgg caagcgcagc
    24961 gtcagcggcg tcttggcctg gtccttaccg agccaaggtc gaatggcctc acccaaaaaa
    25021 cgcagcgtgt aggaaagcgc gtctcgggtc ggagtgggat gcacgagcgc ccagagctcg
    25081 gagagccgca gctcctgcga ccaggccaga tagtcgccgc tcacctcggc caactcgatc
    25141 caagactgcg ccgggttgcc ttcgagcagc gcgtgcacat ccgcgtctgg gttcgtactc
    25201 tcgatgacga gcgagctcga ggcctgccaa gcctcttcga gcaacagcgg gagcaggtgc
    25261 gcggatgccg ctagttccgt actcaaagcc aagctcgcgg ctgccaccac ggggaagcgt
    25321 cgccccgcgc ggtctccgct cggggcaatc gcgccagcca acgcgacgtt cgcgggcgta
    25381 ctcggtggcc gtagcacgaa ggcttgtatg cccccgtgct gcacggcgta gtcccactgc
    25441 ggccgcgcgc gcgacagcgc gaaatccacg ttctcggaga gccactcttc gagctgcatg
    25501 aactccgggc gttgcgcccg aatgcgcacg taatcgcctt gcgagggcac cttgccgatc
    25561 aggcccaacg ccgaaacacc gagcgcgctc atccgccccc gcctccgccg gccggagcca
    25621 ccgccgacat cgtgaccggg gggcagcgca agcgcttgaa gaaacctggc gagaagggat
    25681 gcacgctctt ggatggcttg atgtcgatca ccacgggtga ttcacccgtg cgggtgagca
    25741 agaacgagct cgtgagcaag ccgctctccg cgccggacag ggccttgatg ttgccggccg
    25801 cgagcaagcg gaacaggccg aaatccccga gccgcgggat ttgatcctgg aaatctgcgc
    25861 ctttcaccag caaggtgccg cccttcgggt tgcccttgcc cggccagatc acgggcacga
    25921 agcgctcggg ttcattgcgg tacgtggtgg gggtgccgtc cacgttcagc tggatctccg
    25981 cgatgtttgc gcccaccggg tggatgcgaa cttggaatga aagcgccggt tcggtgccag
    26041 ttccgaagat cgcgtccgtg atctcttgcg agacgttcag gcagctcatg aagtcggcgc
    26101 gataaggcaa cggatcgcgc aggttcttct gctgaaagct attgcccctg cgttcgaaga
    26161 ccttctccaa gttggtggag aagtacttcc acaggatgcc agcttgaggc cgaaagaaat
    26221 ctgcgaactc ggctagagaa acctcggtgg gggagtctgc aaagggatac ttgcccgcga
    26281 tcttttggtt atagacctcc caaacctcgg tcttccactt gtcgctctcg taggtacccc
    26341 cttgagtcac gacgaggatg cggctgccgt aaatcgggtt catcagccac ggctcgacga
    26401 tcgcacgcat ccccagatcg agcctcccga ggagcgctcg cacggtcgac tccgtaccct
    26461 tgatgtcagc gtcaatctgg gcttccggat cctcgatcga gcgccgcagc gcgagctcga
    26521 gcttcgacaa ttcggtgatg tactgctgta acttcgaggg gcccgccgcg ccgcccgccg
    26581 ccccagcggc ggccggtggc gcgtccatcg cggcgaactg caacatcggc atgaatgcgc
    26641 gctcgaccgg agtgatctcg cgctcgggta ccgccgcggc gtcctcgccc gtgagcggct
    26701 tcgtcgcgct attcaccgcg tcgacgccct tcttgatgag cttgtctact aggccgctcg
    26761 gggtcgcatc caggcgaaca ttttgagcga gcacgcggaa gagcgagtcg taaggcccgt
    26821 tcgtctccgt cagcgcgccg agctcggtca gcgcttcttg gttattctga ggtcgtttga
    26881 cttcgagccc caccaagaac gtccgccaag cctgctcgta gcgctggaag tacagctccc
    26941 gcaggcgcgt agcactgctc tgcgcttcct cgtccatctg aatggggccg ctcaagccga
    27001 gcacccacgg ctcgatcacg agccgcccat ccttcgcttc cagcaaaggc ttcacttttt
    27061 gccaaccgag cagcgtatac agccccggca cctcggcgtt ggggggcgcc gtccagtaac
    27121 gcagtgaagc cgcgcggaag atctgatcgg gtcgaatcgc aggcacgccg cgcgccgcca
    27181 gctgcaacca gctgtagcgc agttcgtcga tcggcatctt gctcagctgc ccctgcgcgc
    27241 gggacacgat ggtcagctcc ttgcggaaag cccagctcgg atccgcctgc agcgccttga
    27301 tgtagtaggc actgtgtgcg agcagcttgt ccttgtctgc atcgctgtcg gatcgcagcg
    27361 cccgggccca tacgatggag aggtgctcgg tagcccactc cggctgcaag cgctccaagt
    27421 cgcagagcat gatgtagagc ttgacgttgt cgtagctctc ctgaaagttg tccgcgtcca
    27481 tgcggttcag atcggcgacg cggcgcacgt ctgccacgag ctgcttctgc acggtgccgg
    27541 ccaccatcga gcgcaagcgc tccaagtaga tcttcttgac cgcgaccagc agctccggag
    27601 ccttgtaagg cccccagaac gcatacagtt gcgcttgctc gctggctcgc tccaaaatct
    27661 ggacgcggtc caccaacaga tcgagtacgg aggccgtggc cgttgccgtc gcgttgggtg
    27721 agcgttcgag cgccaccgct tctttcacgg ccgcgtccgt cagtcgaatc agctccgcgt
    27781 tacgcacgta gctcaacgcg ggcggcgtaa tcaccgcgag ggtgccgaac acgaatccgc
    27841 ccgccagcat gacttgccga cggaagcgac gtcgcacccg gctctccgag cgcatcgcca
    27901 ggtggcgatc gggaaagacc accttctgaa agagctcggt gacgaagtag ctctggggcg
    27961 gcccctgacg ttgcgcggcc gggttgccgg cgacctgcag gttgagccca cgcgagatgt
    28021 tgtcgagcaa gcgatcgaac gagcgacccg tctgcgtacc gctcgagaag tagaagccac
    28081 gcagcagcgg cgcatcgttg tggaaattcg gacggcacag ctcctccacg aagcgagcca
    28141 ggttcgcctt gagcgactgg aactccatcg ggaagctcaa gatcttcgag cgcacctcgg
    28201 gcagcggctc acgcgagagg cgatcgagca tgcgcgcgtg tagcacgcgc actaggagct
    28261 cgaactcggt ctcgatagcg cgcgccggct cctccaaacg atcgtcttcc agcgagaagc
    28321 tcgtgcccca ggcctgggcc cgctgcgcct tgctcaagtc ctggaagaac tccgtgaagc
    28381 caccaagcaa gtccaccttg gtgaacatca cgtagattgg gagcaccatc tcgaggtgtt
    28441 tggtcacctc gtcgatgcgt gctcgtagcg tctgcgcctg ttcctccagc tgaacttcgc
    28501 tcaaattcag cagatcctcg acgctgagcg cgaccaccat gccgtcgagc ggacgctcgg
    28561 cgcgaaactt cgagacggtc gtgaggaagc tcagccactc gtcgcggtcg tcttcttgcg
    28621 tcgccagacg gcccgccgtg tccagcagaa tagcctgatc ggagaaccac cagtcgcagt
    28681 tgcgggtacc ggccgtaccg cgcaccttgc cggccgaggc cccgttgggc gtgatgaagc
    28741 ccaagcccga ctgggtcaac gccgtcgttt taccggccgc gggcgggcca atgatcacgt
    28801 accagggcag cgcgtacaga gccgagcgac cgccacgttt gccgagacgc gagcgcttga
    28861 gcgcttcgat cgccgtcttc atctgctgct gcagcgccag aatttctggc cgacggtcgg
    28921 gccgtgcttt gtccgcttgc tgcgccgctt gcttcaagag ctcgcgctcc aaggccgaag
    28981 cacgcatcag cgcgcgcacg cggcggaaca cgaacacccc cacgaccacc agcacgaaga
    29041 agacggacgc gacgatggcg atccaaatcg gccacgtgaa gatcagcacg acggcccatg
    29101 acgccgcggc gagcaccaaa caaatcagag ttacccagag catgggacgc ctcgaagaag
    29161 ctcggcttca gggagcataa cgagcactgc cgagcggcat ggcttcgagc acgtcacgcg
    29221 cgctccccgc cagcagcctc tccagcaaaa acagcagcaa tgcgctggag aacacgcatg
    29281 ccacggccag gattaggatc gtgagcccgc tgccacgcgc ggcttgggcc cgatctttcg
    29341 gttcgacgtg cggcccgaat ttgttggctg acgggagacc gcgctgaatc tgctgccgcg
    29401 cggcctccac gaagccctgc aagctctgca tgtcgccgct cacgccatac gcgccgcgaa
    29461 aaccgagcaa caagcacagg taataggctt cgagcgcgat gccccggtta ccttgttgca
    29521 gcaaggcccg cagccgcacg aagaagttct cacccgccgt gaactctcgg tacaagagca
    29581 gctgcaaggg ttgctgcatc cactcggatt tgcccggcca gttcgacttc aggatctgct
    29641 cgtcgatgaa cgccgtgagc gcgtagcgcg cttcggccag atccggatcg ggcacgccca
    29701 cggcgcggcc gcgaccgacc atcgcgtcca gggccgtcag catacgctgt cgcagctccg
    29761 caggcggcgg cagattctgg gacaacggca gctgcgcggc gagaaccagg atgtctccgc
    29821 aggcccagta caccggctcg ttcatcggct tctcgtgaat cagcggagct tgcttggctc
    29881 gattgcgtag agggacagcg tggtgttcga ccagtcgcta tccaccggga ggtacagcgc
    29941 cacggtttga gacttggcga tctcgtccca gtagctgttt tcgcgacgca cgcggaacag
    30001 gcacatgccc ggtttcatgg gcaacgcccc aaccgggttc cattcgatct ctacacgcac
    30061 gccgtgccgc gcctgcttga ccacgtcgaa gatctggttc cagcccgcca tcttgagcac
    30121 ggccggcaca cgctcgcgca ccagagcatc ggccatgttg gattttacgg cgacgaagaa
    30181 ctcgtccgcg accagcttcg gatccgggat gcgtccgatg aacatgccgt ccgggcgatg
    30241 ctcgagtgga atctccacgt acggctgctc gatacctccc ggcaacaaac gcagcacgat
    30301 cgcgaacagc gcttcgaaca cctccccgag ctccaagtag ttgaacttgg ggatgtcggt
    30361 cggctcgatg cccgtcgaga agctgcacaa catacccgcg agctccgcca gagtgacata
    30421 cgcctcctcc ggatgaaccc gcggtgtcgc gagcagatgg gtgagccgag ggatgctgcc
    30481 gttcaaggtg tgcagcaacc agaacttgcg cgtctcactc gcgtgaaaat cgacgttgcc
    30541 tccggcctga cgctggcgcc tctccccgga gagttgttgc gcgcgcgcca cgatgttggc
    30601 cagcactcgc tcgataccgt tggccaggaa tggagccgca cgcaagtgca aaaccggcgg
    30661 caaacgggta tccagcacct ggaattgacc gttttcctgg cgcaagagct ccgccactcg
    30721 aatgacggtg tagccctcgc gtcgctcgtt tccgaaaaac acccgcagat tgggtcgcgc
    30781 ccactccact tcctggggtg agccgccggt gttggtgtcc accacgttgc gaatctcgcg
    30841 cgtgaagcgg cgggcaacgc tgctctccga atcgcccacc actaggccgg acgtatccgt
    30901 ctcttgggag agccccagga gcacttcgat acgcaccgcg tccccgggca gcgtgcgcgg
    30961 cggagggggc ggctcgggcg tgccatctcc acaactcacg ctggcgccgt cgggccagat
    31021 tgcgctgaat ttcttgactc ggaactgacc ggcggagaat gcccgctcgt cgatctcgag
    31081 ctcggtcacg ccgaagtcgt agtgcgcgac gccctgtaga cggtcgcgca gcagggcctc
    31141 gtgataacga tcttgttgct ggaagtggtg ctggctgacc
//
LOCUS       DASTKU010000847         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1282214, whole genome shotgun sequence.
ACCESSION   DASTKU010000847 DASTKU010000000
VERSION     DASTKU010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282214"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..1922
                     /locus_tag="VFQ61_29790"
     CDS             216..1922
                     /locus_tag="VFQ61_29790"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9958736.1"
                     /translation="MRDTAQRFRKDGEGRTVIAEGIMNAKPGSSLMKAAMATDSLLNS
                     EPRDSTPLSPEAYAKGQVLAGKYRVERIIGEGAMGVVLAATHLDLDETVAIKFIRPGM
                     HKVPGILARFAREAKASAKIKSEHIANVLDVGVAAPIGPYIVMEYLEGKDLCDILGVY
                     GKLSVRRAAEYVMQACEALAIAHANGIIHRDIKPENLFLTRRGELEVIKVLDFGISKA
                     ALTGSVFGGELAVNETACMMGTPLYMSPEQIRSTGNVDHRADIWSLGGVLYELLTGRT
                     AFVSDSVAQLCAMVLETVPTPVRELVPEIPEAMAEVIDRCLQKNPDRRFQNVGELAIA
                     LSPFAPSRARLHAERSTSILRASGVALNVDLRPSSMPPARSDTAVSANLLSTGQIPIS
                     TAVLVKDVTPTPASNTAGDIAALRRRHRAVVPALVLALGGVAAVGFFTSRQNTASSAA
                     DSSLIATRAAEALRDAQSQAAAPLAAPQLPTATPGADAQAASLGEMDNKLGSLPRAKL
                     AGRPAQVRTANQRLVTKASPGPTAVHSETVAAAPSTSTKTPAPKPRVRLVDDQTRARL
                     LD"
     gene            2246..3166
                     /locus_tag="VFQ61_29795"
     CDS             2246..3166
                     /locus_tag="VFQ61_29795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958737.1"
                     /translation="MKGSTSMGAAVAAAHKAMVFAGALSAATAYTLGIVQTPLAAAQE
                     AAPSASASPSSTEPGNAEGGNAVDVAQLNEEGSQLYRARDYRHAVEKFILAYAADPDP
                     NLLFNIARCYEAMGDTDVAIEKYETFLTTPGADPQGKQRARDALKRLHETQSNKEASV
                     TPSSRSASSHATSAPPSSAAPSPKLTEGGTSILPWIALGAGAASASVGGVLYLLGVKD
                     HAKVTDTPGYNDASRAASITESSARDLVDSGTRKKTIGGIGLGVGGALLTTSIVLFVT
                     GNSGKNSEQGVALRVAPGTSGGSVSLRGSF"
BASE COUNT          640 a         1057 c         1016 g          572 t
ORIGIN      
        1 cgccttgact ggcgaccggc tgcacgtgcg tgccgtagta gagactcacc aaaggccgca
       61 ctcgtctcga gaagaatgcg agcgatgtgg agaaaaacgt ttcgtccgtc atcctgtaaa
      121 gtgagtggag ctcagcgggg cgcgacggct ctctgacgct gtctacagca gtgcgaaaag
      181 ctccgccaat tcgtgaacgc cctggcggaa ctcgattgcg tgacaccgct cagcgcttca
      241 gaaaggacgg cgagggccgt accgtaattg ccgagggaat catgaacgca aagccgggat
      301 cgagcttgat gaaggccgct atggcgaccg actcactttt gaattccgaa ccgcgcgact
      361 cgacaccgct ctcaccggag gcttacgcca aaggtcaggt cctggccggg aaatatcggg
      421 tcgaacgcat catcggcgaa ggcgcgatgg gggtggtgct cgccgccacc cacctggatc
      481 tcgacgaaac ggtcgccatc aagttcatcc gtcccggcat gcataaagta ccgggaattc
      541 tcgcgcgctt cgcgcgagag gccaaagcct cggccaagat caagagcgaa cacattgcca
      601 acgtgctgga cgtgggtgtg gcggcgccga tcggccccta catcgtcatg gagtacctcg
      661 agggcaaaga cctctgtgac attctgggcg tctacggcaa gctctcggtg cgacgtgcgg
      721 ccgagtacgt gatgcaagcc tgcgaggcgc tcgcgatcgc gcacgccaac ggcatcatcc
      781 atcgtgacat aaaaccagag aatttgtttt tgacccggcg cggtgaactc gaggtcatca
      841 aggttctgga tttcgggatc tcgaaggccg cgctgactgg ctccgtattt ggcggtgaac
      901 tcgcggtcaa cgagacggcg tgcatgatgg gcaccccact gtacatgtcg cccgagcaga
      961 tccgttcgac gggcaacgtc gatcatcgtg ccgatatttg gtccctcggc ggcgtgctct
     1021 acgagctctt gacgggacgc accgcgttcg tctcggacag cgtggcgcag ctctgcgcaa
     1081 tggtattgga gacggtaccc acgccggtgc gtgagttggt gcccgagatc cccgaggcca
     1141 tggccgaggt gatcgatcgt tgcctgcaaa aaaacccgga ccgacgcttc cagaacgtcg
     1201 gggaattggc gatcgcgttg tccccattcg cgccatcgcg ggcacgcttg cacgccgaac
     1261 gctcgacctc gatcctgcga gcttcgggcg tcgcgctcaa tgtggatttg cggccgagca
     1321 gcatgcctcc ggcgcgttcg gataccgcag tttcggccaa cttgctctcg actggtcaaa
     1381 tcccgatctc cacggccgtg ctcgtaaaag acgtgacccc cacaccggcg tcgaacacag
     1441 ccggtgacat tgccgcgctc cgaagacgcc atcgagcggt cgttcccgcg ttggtgctcg
     1501 cgctgggcgg cgtcgcggcg gttgggttct ttacgtctcg tcaaaacacc gcgagcagcg
     1561 ccgccgattc gagcctaatc gcgactcgcg cggccgaggc gctgcgtgac gcgcaaagcc
     1621 aggccgcggc gccgttggcg gcgccccagt tgcccacggc cacccccgga gccgacgcgc
     1681 aggccgcttc gctcggcgag atggacaata agctcggctc tttgccgcgg gccaaactgg
     1741 cagggcgccc cgcgcaagtt cgcactgcca atcagcgcct tgtcacgaaa gcgagccccg
     1801 gccccacggc ggtgcacagc gagaccgtgg ccgccgcgcc gagcacgtcg accaagaccc
     1861 ctgcacccaa gccccgtgtg cgtttggtcg acgaccagac ccgcgcgcgc ctgctcgact
     1921 agtgatttct catgtgatgt tttctgccgg cccgatgtcg ctcgcagagt cacacaatat
     1981 tgtccgtcga atgctccaat aaattttgtg cacccgcttc agtctggtgt gcacctcgtc
     2041 gtaggcagtt cggaactcat cagcatctcg agatcggcgg cggcccgtac gctacaggcc
     2101 aaagcaccta tgacatcggc ggcaggccga tcgcctcttc tttcgagatg acattcaacg
     2161 cacgctcgcc aagcggcgca ccgcagaggg taaatcggct gcgctgagtg cctgcgagca
     2221 aaacgactcg gatcaggagg gcatcatgaa aggttcaaca tcgatgggcg ccgccgtcgc
     2281 ggctgcgcac aaggccatgg tgttcgccgg tgcactgtcg gcggcaaccg cctacacgtt
     2341 gggaatcgtg caaacgccac tggcagcagc tcaagaagcc gctccctccg cttcggcctc
     2401 tccttcatca accgagcccg gaaacgccga aggcggcaac gccgtagatg ttgcgcagct
     2461 caacgaagag ggctcacagc tctaccgcgc ccgcgactac cggcacgcag tggaaaaatt
     2521 catccttgcc tacgccgcgg atcccgatcc caacctgctg ttcaatatcg cacgctgcta
     2581 cgaagcgatg ggagacacgg acgtcgcgat cgagaaatac gagacgtttc tcaccactcc
     2641 gggcgccgat ccgcagggca aacagcgcgc gcgcgacgcc ctgaagaggc tccacgagac
     2701 ccaatccaac aaggaagcca gcgtcacacc gtcgagccgc agcgcgagct cgcacgcgac
     2761 ttcggccccg cccagctcag ctgcgccatc gcccaagctg acggagggag gcacctcgat
     2821 attgccttgg attgcgctgg gagctggcgc cgcgagcgcc agcgtcggcg gcgtcttgta
     2881 cctgctcgga gtgaaagacc acgccaaagt aacagacacg ccgggctaca acgatgcttc
     2941 gagggccgcc agcatcaccg agagctctgc gcgcgacctc gtcgattcgg gaacacgtaa
     3001 gaagacgatc ggcggcatcg ggctcggcgt cggcggcgcg ctgctcacga cgtctatcgt
     3061 gttgttcgtg acgggcaact cgggaaagaa ctcggaacag ggcgtggcgt tgcgcgtagc
     3121 gccgggcact tccggcggct ccgtctcttt gcgcgggagt ttttgagatg cttcgcaaag
     3181 tcgctttgtg gggtggcacg tggctaaccc tctcgtgctc agcgccgtcg ctcgagggaa
     3241 cgaacttcgc ctgccaaacg gacgcggatt gctctggcgg taagg
//
LOCUS       DASTKU010000848        13124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1953250, whole genome shotgun sequence.
ACCESSION   DASTKU010000848 DASTKU010000000
VERSION     DASTKU010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13124
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953250"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VFQ61_29800"
     CDS             <1..1108
                     /locus_tag="VFQ61_29800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958738.1"
                     /translation="LEQPICTLNRYINRPAPELPKHAVFLVLSDEDDISKPEQCLAQY
                     SYHPSPSGVTDSECQTGCDWYDYSMQFQQALQTIEARCVEVDDQGVPHPESYSTIFGA
                     NSANICDPNEHACSADDIALLKNACGPDSILGECSAKCDGGAASIYSCTLRRPDLSLD
                     LCNEAFTEGGTTYRNMADYCQRAQPDKSPWQECSVTGIKQGGRTLYDGQDVLTKVVEA
                     SSLSDMIESFHDRARSAFGATGYFVESVIFDPSFSCSLQSGQSYGATLRQLASSPADV
                     FPICSDYAPALQRIQGFAQKLVQNEFKLELKSDEELESVTVHSLAGGTRMLAATEYSF
                     DRSTAKLVLKPGTLAPSDSTLDLLIADTCVELVR"
     gene            1606..3411
                     /locus_tag="VFQ61_29805"
     CDS             1606..3411
                     /locus_tag="VFQ61_29805"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9958739.1"
                     /translation="MKWCRSIACSRLWQLVLGLAVAPSCAGVVEDPGSSPVEPNPGNV
                     GGNSSASLGGSSGNTSVAPGQGGANGGSTASAGSPSTGMDCVNPNPGSAPLRRLSNAE
                     YRNSLRDLFKTVNGMDAEITSATQGLPAEAESLGFRNSAEFLTVQPLLAQNYESAAER
                     LAAKAASTPGFVPCTPKPSEELACAKQLITKFGKAAYRRSLSSAESARYEAMFQSGFK
                     EKDFTTGIEWVVLGMLQSPGFLYRAERGVAGTPTGSSGGPRQLSGYEMASRLSYLFWQ
                     SAPDEALLALAEGGAFATPQAVEKQARAMLADSRADRLFEYFAEWLDLDHLDEFARDA
                     SVFPNLPTQLPDWYRQETQSFVRALLSNPQGSLNELLTAPYTYANKGLASHYGLTGPT
                     GDAFVRVDDARRSGVLTQAMLMSRDKPYRTSIVRRGLKIRTDFLCQNVPAPPNDVQLN
                     LDSAAATLSQRDRLAQHRKDPSCAACHNLLDPIGVVFENFDAVGRYREKDELNQSIDT
                     SSELNATRDSNGPIGNVRELGASLARSAEVQDCYVKQSFRFFFGREVEAADACSMAQL
                     QNKFRNTNQSLSELLVALTQTDAFLYLPPVKEVAP"
     gene            3408..4781
                     /locus_tag="VFQ61_29810"
     CDS             3408..4781
                     /locus_tag="VFQ61_29810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9958740.1"
                     /translation="MKRLSRRTLLRGAGGFALTLPFLDVMRPDRAQAQTVAAPRRILF
                     VFQANGDQVNARFASTGETAFTLGEFLAPLEPHRNDLLFLNKVHRRFYALPEAERADN
                     HQQGGSSLAPWTSGEGSFPIGGAEGKTIGYVEGPSADFAIGERVLALKQGVAHRHLVY
                     RVGDKSNNIWNLHSHAGPAGQQNPVPPETDPYAAYARIFSFGSNVAETEAALRLRLAK
                     RKSGLDLVRTEIASLKSELPAADQRKLDQHTEAIRDIERTLSEGNSTAACTPLTLGSK
                     IDPYKDENHALVGELFFKISALAFACDLTRSINFNWSGNTSGRVYANLGLSQGHHDIS
                     HDSSDTAFADIRKVHKHLWTQNTKLYELLKATPDGSGTLWDHTLVVHWNELGQGDRHS
                     IEDTLVVLAGGAHGFFKKNRLLDFGGKASFSDVLLTCFHYMGFEDVTEFGDSRLKLKG
                     LLSELKA"
     gene            complement(4786..5841)
                     /locus_tag="VFQ61_29815"
     CDS             complement(4786..5841)
                     /locus_tag="VFQ61_29815"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HET9958741.1"
                     /translation="MIPKREPSEEMPKRELTEELATFHERASATSLRLRSPSASRASA
                     EGGPLDRGSTQSKPDAAVDEARGELALAMHQAWLIDVITTPESRPLPVDADAAERLVM
                     SSARLSALDRLELYRQSYHARLLECLADDFPVLREAAGVEGFEWLARGYIAEHPSRGP
                     NLNEFGRHFPGFIRTARWAERPREGAPELTALPPVEFLADLAALEWALVVAVHAPSCP
                     PMTPADVARVPPEGFPTARLVPNPSLSILHFDYPVNAFFQRLRHGEGASVPAPRESAV
                     AVYRTERKVYRLELSAGMSALFESLARGTPLGEALELTFRKLQAHGAGEAELARMVMQ
                     GFRDGVESGLFAEIRSG"
     gene            complement(5838..6740)
                     /locus_tag="VFQ61_29820"
     CDS             complement(5838..6740)
                     /locus_tag="VFQ61_29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HET9958742.1"
                     /translation="MNRFGLPDLGIGVGLRTVHYATILEQRPDVGWFEIISENYMQTA
                     GRPLEFLDAIAERYPIVMHGVSLSIGSTSPLDRGYLSELRALRDRVAARWVSDHLCFT
                     GSASKNTHDLLPLPYSEATLRHVVDRVREVQDLLGAPLALENPSSYAQLSGGSMPEWE
                     FLARLAEAADSAILLDVNNVYVSAYNHGFDPEQYLAAMPFDRIVQLHVAGHTQYDTHI
                     LDSHIGPVIDAVWQLLAQALERNPEIPVLLEWDAEIPSFEVVHREALRAAEYRGPIAT
                     CTPGGTRPGSAPARSPIGIGVSER"
     gene            complement(6794..7135)
                     /locus_tag="VFQ61_29825"
     CDS             complement(6794..7135)
                     /locus_tag="VFQ61_29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958743.1"
                     /translation="MNFSNALMAAAISGILAGASGCAENPPPADPSTPPTAEGTLPAA
                     AGSETAAPPAAPADAAAAPAGAAGETAPAGEKHACKGMNSCKGQGGCKSDKNACKGQN
                     QCKGQGGCKTG"
     gene            7411..9111
                     /locus_tag="VFQ61_29830"
     CDS             7411..9111
                     /locus_tag="VFQ61_29830"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9958744.1"
                     /translation="MSESRTIRLLAFARKLQNAATFFDLLQIAREEVRDAVGYNHAWL
                     VVADEEGANELRLIDVSGVQRELVWEVAPKIPIHGDPLVEELMQSDVPIVIPEAADDL
                     RTNKQVVAQLKNRTLINIPLRLLDKPFGAFGIGTFGDEGVRVPSPDELDYLVGMASQL
                     AVAASRIRFIEGKARAQREREDFERRLMQVQKLESLGMLAGGIAHDFNNLLTVITASA
                     SLAERQTADTMLLAELQSIAGAAKRASALTRQLLAMSRSQDLDLKPLDLNSQLRQLLE
                     LVRRVLPENVTIDFIEGQALPLIEGDASQIDQVFMNLFVNARDAMPEGGCLTVESEQV
                     VVNGRYVEAHPWAQPGRYVLVTVTDTGVGMTREVLDRVFEPFFTTKGPRAGTGLGLAV
                     AYGIVRQHRGMLHAYSEVGVGTSFKIYLPALERLATAVGTKLQRAIVRGSERILVAED
                     DELVRAVAVRILERAGYRVTAAEDGDAACVAVGKDTYDLVILDVVMPGAPCREVMQRI
                     REISPRTAILLSSGYTAGEKITSLVQETKLDLLRKPYDPDTMLVAVRAALSLGRGLVQ
                     "
     gene            9235..10470
                     /locus_tag="VFQ61_29835"
     CDS             9235..10470
                     /locus_tag="VFQ61_29835"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET9958745.1"
                     /translation="MRLTEAASREGVDESPRGVFVTARGALSSLGANAAEHLIGYRDG
                     SLGLHPLQVGSYRVPLGALSASARARVSELVGAHPQYARLDPSVLFAILATREALATK
                     PELESFAVCLGSSRGATERLEQTHRDFLNHGEVKPATSPTTTLGNLSSNVARDRFGPN
                     VSVELSSACTSSLHAVLFGYAYLRAGLGAACLVGGAEAPLTPFTLAQLDALGLLNRDL
                     ESATPCRPLSGAASATPESTMNRMVLGEGACVLLLQRLPRSEAARRGALAEIVGIGSA
                     LEKTPHPASMSADGQALQQAMRAALAAQVAAVDAVVVHAPGTARGDKAELAALAKVFG
                     SHVPPLCSNKWQLGHTFGASGAFGIDFALALLAGAVPATAPFPSALSQPTGAVRRVLV
                     NGAGFGGTAVSVLLQAVAN"
     gene            complement(10503..11252)
                     /locus_tag="VFQ61_29840"
     CDS             complement(10503..11252)
                     /locus_tag="VFQ61_29840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958746.1"
                     /translation="MMTPASVSGPRLETSAGIAELGGRVVVWGRDLHHECLDFSFVQY
                     LLFSVTGREFSVSQARVFERLWISTGYADARIWCNRIAAYMGSARVPPSLSMCAAIAA
                     SNSVGYGFGALRDAYAVQLEIPENSAEREAWLAAVFADRRVLAGYGRPIHNGDERIPV
                     ALHILTEEGVSAGPALRRAFWLHFRLRERKGIQMNIAAVFAAIGADFGLDAREFEQFM
                     LLMLTPGYSAVYADQRERPAFTFLAGHQSLR"
     gene            complement(11249..11926)
                     /locus_tag="VFQ61_29845"
     CDS             complement(11249..11926)
                     /locus_tag="VFQ61_29845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958747.1"
                     /translation="MKAQLYSYLKSLFSEPGLFDEGVEAARNACVAGASLASALITAA
                     AWYENPLTGTPALLDALDRLRSIPGHGSPSCAGFVGAFAPTPHRDTGDPEHSPGFGYI
                     DEERADRVVRAGQNLIAQFPGAFRALEFFLEQRRQIVTRCGALNLTGLCALGFHGAGL
                     TADEAERSFLVLRVEAALREAQHARALGLKGFPFFSELYRYQGPLPEARGFDLEQLMT
                     RVGISLP"
     gene            complement(11923..>13124)
                     /locus_tag="VFQ61_29850"
     CDS             complement(11923..>13124)
                     /locus_tag="VFQ61_29850"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET9958748.1"
                     /translation="MVTAPMGPVHLSIPSDVLAGPGALSDSGLAQALRAPEVADLDAI
                     DELLNRVVKAKCPVLYVGDDAGPKAFQLATVAFRLGAQLVSSPAGKRWVSHTTACYKG
                     VVGFSGHPEAREAIHRADLVVTFGATFDELSTNAWGAFPDVPVYAVDRHAEHVHRLPS
                     ARAVIANPARVIDALLQRLPPTSAVLMRPPRRPTPAMMVQSVPDGPVHPKDLMHWVGL
                     VLPSDVAVHVDAGNSFSWSTKNLARSTPDTYRVAMGLSTMCWAISAAIGATVATHRRT
                     ICITGDGSMLMSSLELTVAVEQNLPVTYIVLNDSSLGMVRHGQKLAGAESVAHQIAPV
                     RFDQLAKACGAEGIRIDTLSDLGRIARRWLALDEGGPCLIDVNIDRAAVPPIGDRVVG
                     LAVGVTR"
BASE COUNT         2515 a         4200 c         4073 g         2336 t
ORIGIN      
        1 actcgaacaa cccatttgta ctttgaatcg gtacatcaat cgacccgccc cggagttgcc
       61 aaagcacgcg gttttcctgg tgctttcgga cgaggacgac atctcgaagc ccgagcaatg
      121 cctggcccaa tactcttacc acccgagccc gagcggagtc acggattcgg aatgccaaac
      181 tggctgtgat tggtacgact attccatgca gttccagcag gcgctgcaaa ccatcgaggc
      241 gcgctgcgtc gaggtcgacg accaaggggt tccccatccg gagagctact ccacgatctt
      301 cggagccaac tcggcgaaca tctgcgaccc caacgagcac gcctgcagcg cagacgatat
      361 cgcgctgctc aagaacgcct gtgggccgga tagcatcctc ggcgagtgca gcgccaaatg
      421 cgatggtggc gcggcgtcca tctattcctg cacgctgcgc aggccggatc tcagcctcga
      481 tttgtgtaac gaggccttca cggagggcgg gaccacgtat cgaaatatgg ccgattactg
      541 ccaacgcgcc caacccgata agtcaccttg gcaggagtgt agcgtgaccg gtatcaaaca
      601 gggtggcagg acgctctacg acggacaaga cgtgctcacg aaggtcgtag aggcgtcctc
      661 gctctcggac atgattgaaa gctttcacga ccgggcccgc agcgcatttg gcgctacggg
      721 ctacttcgtg gagagcgtga tattcgatcc cagcttctcg tgttcgctcc agagtggcca
      781 aagctacgga gcgactctgc gccagctcgc gtctagcccc gccgacgtct ttccaatctg
      841 cagcgactac gcccccgccc tgcaacgcat ccagggcttc gcgcagaagc tggtccagaa
      901 cgagttcaaa ctcgaactca agagtgacga ggaactggag tccgtcacgg tacactcact
      961 ggctggcggc acacgcatgc tggcggccac ggagtacagc ttcgaccgga gcaccgcgaa
     1021 gctggttttg aagcccggca cgctcgcgcc ctcggacagc acactcgacc tcttaatcgc
     1081 agatacgtgc gtcgagctcg tccgctgagc ggtcccgaga cagcctgtct acggcttttg
     1141 ggaggtccgc gcgctcgccg gactcttccc tgtagctgtc ggtcccttgg gggtggtcgc
     1201 ccggcggttc gcagccgatc gtcgatctgg ctgagctcga gccgcagccg ctgatgtctg
     1261 cgagcggcat ggatttatgc ggagtcacgc gaagaacccg gcgcgtgttg cacaactcgt
     1321 tcagggttga acttcgagtc acgcttcgcg gcgccacgaa gccgtcaagg taggagaaat
     1381 cgaactcgag atctgcctgc tgacgaattg gtggagtgca cgacctgaat cggttgctac
     1441 cctgctgggt gcgtttggaa tcatagagcg tcttggcgcg cctgaactcg gggtctgcgc
     1501 tgagtagtga ttcgcctcca acctgaattg gctccagcct gcactggctc caacccgcat
     1561 tggctccaac ccgcattggc tccaacccag aagggcccga ttcgcatgaa gtggtgtcgt
     1621 tcaatcgcgt gctctcggct gtggcagctc gttttgggac tcgccgtcgc cccgagttgc
     1681 gccggtgtcg tcgaagatcc gggcagttcc cctgtggagc ccaaccccgg taacgtgggc
     1741 ggaaactcct ctgccagtct cgggggctcg agtggcaata ccagcgtcgc tcccgggcaa
     1801 ggcggcgcga acggcgggag taccgcttca gcgggctccc cgagcacggg catggactgc
     1861 gtaaacccga acccgggtag cgcgccactt cggcgtctca gcaacgcgga gtatcgcaat
     1921 agcttgcgcg acttgttcaa gaccgtgaat ggcatggacg ccgaaatcac gtctgccacc
     1981 caggggctgc ctgcagaggc cgagtcactc ggattcagaa acagcgccga gttcctgacc
     2041 gttcagccgc tgctcgccca gaactacgag agcgcggcgg agcgcctggc cgccaaagcg
     2101 gcgagcacac cgggcttcgt cccctgtacc cccaagccct cggaagagct cgcgtgtgcc
     2161 aaacagttga ttaccaagtt cgggaaggcg gcgtatcgtc gctcgctgtc gtccgccgag
     2221 agcgctcgct acgaggccat gttccagagc ggattcaagg aaaaggactt caccaccggg
     2281 atcgagtggg tcgtgttggg gatgctgcag tcacccgggt ttctttaccg ggcggagcgc
     2341 ggcgtagccg gtaccccaac ggggtcctcc ggcgggccga ggcaactctc gggctacgag
     2401 atggcgagtc ggctctccta cttattctgg cagtccgcgc cggacgaagc gctactcgcg
     2461 ctcgcggaag ggggagcgtt cgccacgccg caagcggtcg agaaacaggc tcgggcgatg
     2521 ctcgcggatt cgcgcgctga tcgacttttc gagtacttcg cggagtggct ggatctcgat
     2581 cacctggacg agttcgcgcg cgacgccagc gtgttcccca acttacccac ccagctgccc
     2641 gattggtacc ggcaagaaac gcagagcttc gtacgagcct tgctgtctaa ccctcaaggg
     2701 agcttgaacg agctgctaac cgccccctac acctacgcca acaaggggct ggcgagccac
     2761 tatggcctga ccggtcccac cggagatgcc ttcgtaaggg tggatgacgc gcggcggtcc
     2821 ggcgtgttga ctcaggccat gttgatgagc cgggacaagc cctatcgcac ctcgatcgtg
     2881 cgacgaggac tcaagatccg caccgacttc ctgtgccaaa acgtacccgc tccgccgaat
     2941 gacgtgcaac tcaacttgga ctctgcggcg gctacgctct cgcagcgcga ccgcttagcc
     3001 caacaccgta aagatccgag ctgcgcggcc tgccacaacc tactcgatcc gattggggtc
     3061 gtattcgaga acttcgacgc ggtcgggcgc taccgggaga aggacgagtt gaatcagagc
     3121 atcgacacca gcagcgaact caacgcgacc cgcgacagca atggcccgat cggaaatgtc
     3181 cgagagttgg gagccagcct cgcgcggagc gccgaagtgc aagactgcta cgtgaagcag
     3241 agctttcggt tcttcttcgg tcgcgaagtc gaggccgccg atgcctgcag catggctcag
     3301 ctccaaaaca aattcagaaa cacgaaccaa agcctctcgg agctcctcgt ggcgctcacc
     3361 caaaccgacg ctttcctcta cttaccgccg gtgaaggagg tggcgccatg aagcgcctgt
     3421 ctcgaagaac tctgttgcgt ggcgcgggag gtttcgcgct caccttgccc tttctcgacg
     3481 tgatgcgccc ggatcgagcc caagcgcaaa cggtcgcggc gccacgacgc atcctgttcg
     3541 tgttccaagc caacggcgat caggtcaacg cgcgcttcgc aagcacgggt gaaaccgcgt
     3601 ttacgctcgg tgagttcctg gcgccgctcg agccgcatcg gaacgacctg ctgttcctca
     3661 acaaggttca tcgtcgcttc tatgcgttgc ccgaggcgga gcgcgcggac aatcaccagc
     3721 aaggcggcag ctcgctcgcg ccctggacga gcggagaagg gtccttcccg attggcggcg
     3781 ccgagggcaa gacgatcggc tacgtggagg ggccgagcgc cgatttcgca atcggggagc
     3841 gtgtacttgc gctcaagcag ggagtcgcgc atcgccacct cgtgtaccgc gtgggggaca
     3901 agtccaacaa catttggaac ctgcattcgc acgccggccc ggctggacaa caaaacccag
     3961 tacctccgga gaccgatccg tatgcggcat atgcgcgcat cttttcgttc ggttccaacg
     4021 tcgcggagac cgaagcggcg ctacggctgc gcttggcgaa acgcaaatcc ggtctggatc
     4081 tcgtgcggac cgagatcgct tctctgaaga gcgagctgcc cgccgcggac cagaggaagc
     4141 tcgatcaaca caccgaggcc attcgcgaca tcgagcggac gctcagcgaa ggcaactcga
     4201 ccgcagcgtg cacgccgctc accttgggga gcaagatcga tccgtacaag gacgaaaatc
     4261 acgcgttggt cggggagctc ttcttcaaaa tctctgcctt ggcctttgct tgtgatctca
     4321 cccgttccat caacttcaat tggagcggga acaccagcgg ccgggtctat gccaacctcg
     4381 gcctgagcca aggccatcac gatatctccc acgacagctc ggacaccgct ttcgcggaca
     4441 tccgcaaggt ccacaagcac ctgtggacgc agaacaccaa gctttacgag ttgctgaaag
     4501 ccacgcccga cggcagcggg accctgtggg accacaccct ggtcgtacat tggaacgagc
     4561 ttggacaagg tgaccgccac tccatcgaag ataccttggt cgttctcgcg ggaggcgcgc
     4621 atggcttctt caagaagaac cgcctgctcg actttggcgg caaggcctcg ttcagcgatg
     4681 ttctgctgac gtgtttccac tacatgggct tcgaggacgt caccgagttc ggtgattcgc
     4741 ggctgaaact caaaggcttg ttgtcggagt tgaaggccta agcactcatc cactccgaat
     4801 ctcggcaaac agtccactct ccacaccgtc gcgaaagcct tgcatcacca tgcgcgcgag
     4861 ctccgcctca cccgctccgt gcgcttggag tttgcggaac gtcagctcca aggcctcccc
     4921 gagcggggtg cctcgtgcga gcgactcgaa cagcgccgac attcccgccg aaagctccaa
     4981 tcggtacact ttgcgctccg tccgatatac cgcgacggcg ctctcgcggg gtgccggcac
     5041 gctcgcgcct tcaccatgcc gcaggcgttg aaagaaggca tttaccgggt aatcgaagtg
     5101 caggatcgaa agagagggat tgggcaccag ccgcgccgtc gggaatccct ccggtgggac
     5161 acgcgccacg tccgctggag tcatcggcgg acagctcgga gcatgcaccg ccacgactaa
     5221 ggcccattcg agcgcggcga gatccgccaa gaactcgacg ggcggaagag ccgtaagctc
     5281 aggagcacct tcccgaggcc gctccgccca acgcgccgtt cggataaagc ctgggaaatg
     5341 gcgaccgaat tcgttcaaat tgggccctcg agaggggtgt tcggcaatgt atcctcgggc
     5401 gagccactcg aagccctcga ccccggccgc ctcgcgcaga accggaaagt cgtccgccag
     5461 gcactccagc aagcgggcat gataactctg ccggtaaagc tccaaccgat cgagcgcgga
     5521 taaccgagca gagctcatca ccagtcgctc ggctgcatcg gcgtccacgg gcaatggccg
     5581 cgactctggg gtggtgatca cgtctatgag ccaagcttga tgcatagcca acgcgagctc
     5641 cccgcgagct tcgtcaaccg ccgcatcggg tttggactgc gtagatcctc ggtctagcgg
     5701 ccctccttcg gcggaggccc gactcgcgga tggtgaacgc aagcggagcg aggtcgcgct
     5761 cgcgcgctcg tgaaacgtcg ctagttcttc ggtcagctca cgctttggca tttcttcgct
     5821 cggctcgcgc ttcggtatca tcgctcactc acacctattc ctatagggct gcgggcggga
     5881 gcagaaccgg gtcgggtccc acccggagtg caggtcgcaa ttggcccgcg gtactccgcg
     5941 gcacgcaacg cctcgcgatg tacgacctcg aagctcggga tctcggcatc ccattcgagc
     6001 aagaccggga tctccgggtt tcgttccagc gcttgggcca gcagctgcca aactgcatcg
     6061 atcacggggc caatgtgcga atccaggatg tgtgtgtcgt attgcgtatg ccccgccacg
     6121 tgcagctgca cgatgcgatc gaacggcatc gccgcaaggt actgttccgg gtcgaacccg
     6181 tgattgtacg ccgacacgta aacgttgttg acgtcgagca ggatggctga atcggcggcc
     6241 tccgcgaggc gcgccaggaa ctcccattcc ggcatgcttc cgcccgaaag ctgcgcatag
     6301 ctcgatggat tctcgagcgc gagaggagcc ccgagcaagt cctgaacttc ccgaacacgg
     6361 tccactacgt gccggagtgt cgcctcgcta tacggtaagg gcagcaagtc gtgcgtgttc
     6421 ttactggcgg atcccgtaaa gcacaggtga tcggacaccc agcgcgccgc gactcgatct
     6481 cgcaatgccc gaagctcact caggtaccct cgatcgagcg gactcgtcga gccaatcgac
     6541 agggaaaccc catgcatcac gatcggatag cgctccgcga tcgcgtccag gaactcgagc
     6601 gggcgacccg ccgtctgcat gtagttttca ctgatgatct cgaaccagcc cacatctggg
     6661 cgttgttcga gaatcgtcgc gtagtggacg gtacgcagtc ccaccccgat ccccaggtcg
     6721 ggcaaaccaa agcgattcat gactaaccca cggtgatacg gccgccccgt gcaaggcggc
     6781 cgttccatca acttcagccc gtcttacagc cgccctggcc cttgcactga ttctggccct
     6841 tgcaggcgtt cttgtcggac ttgcagccac cttggccttt gcaggagttc atccctttgc
     6901 aggcgtgctt ctcacccgcc ggtgccgtct cccccgctgc gcccgcgggt gccgcggcgg
     6961 catctgccgg agcggccgga ggcgccgcag tctcgctgcc ggcggcagca ggcagggttc
     7021 cctcggcggt cggcggagtg gacggatccg caggcggggg gttctcggcg cagccgctcg
     7081 ctccggccaa aattccgctg attgctgctg ccatgagtgc attcgagaag ttcatcgtgt
     7141 gtgctttcct gttgttgatg gtcggatgtc tggcgccgat cagcgcctcc aacctcctgt
     7201 acgcgcccgc accggaccgg ttacatgtca ccggcagaac gggcgcgggg atgctatcag
     7261 gcttgcctgt ccgttcgaca gaaagtacga acgatgagga gcaggtggat cggctgagcc
     7321 atctgcacag ccccaagttg taggtttctg acgtttcggg ccggcttggg gttcaatttt
     7381 tccgaggaga ggatatatct agctgcgcgc atgagtgaat cgcgcacgat ccggttgttg
     7441 gcgttcgcac ggaagctcca aaacgccgca actttcttcg acttgcttca aatcgcccgt
     7501 gaagaggttc gcgatgcggt gggctataac cacgcctggc tggtcgttgc ggacgaagag
     7561 ggtgcgaacg agctgcggct catcgatgtc tccggcgtgc agcgcgagtt ggtttgggag
     7621 gtcgcaccca aaatcccgat tcacggtgat ccgctcgtcg aggaactgat gcagtcggac
     7681 gtaccgatcg tcattccgga agcggcggac gatctgcgca ccaacaagca ggtcgtcgcg
     7741 cagctgaaga accgcaccct gatcaacatc ccgctacgcc tgttggacaa gccatttggc
     7801 gccttcggga ttggcacatt cggggacgaa ggcgtacgtg tccccagccc cgacgagctc
     7861 gattatctgg tcggtatggc gagccagttg gccgtcgccg cgagtcgcat ccggttcatc
     7921 gaaggtaagg ctcgagcgca acgggagcgc gaggacttcg agcggcgcct gatgcaggtc
     7981 cagaagctcg agagtctcgg catgctggcg ggtggtatcg cgcacgactt caataacctg
     8041 ctgaccgtga ttacggcgag cgcctcactg gcggaacgcc aaaccgccga caccatgttg
     8101 ctggcggagc ttcagtcgat cgcgggcgcg gccaagcgag cttccgcttt gacccgacag
     8161 ctgctcgcga tgagccggtc gcaggacttg gacctgaagc cgctcgattt gaactctcag
     8221 ctacgacagc tgttggagct ggtgcggcgc gtgctgccgg aaaacgtcac catcgacttc
     8281 atcgaagggc aagcgctacc cctgattgaa ggggatgctt cacaaatcga tcaggtgttc
     8341 atgaacctgt tcgtgaacgc gcgtgatgcg atgccggagg gtggctgcct caccgtcgag
     8401 agcgaacaag tggtcgtgaa cggccgctac gtggaggcgc acccttgggc gcagcccggc
     8461 cgctacgtgc tcgtcacggt cacggacacg ggcgtgggca tgactcgaga ggtgctcgat
     8521 cgtgtattcg agcccttctt tacgacgaag ggaccacgcg cgggtacggg gttgggcttg
     8581 gcggtagctt acggcatcgt gcgccagcac cgcggcatgc tgcacgccta tagtgaggtg
     8641 ggcgtcggta cgtcgttcaa gatctatttg ccggcgttgg agcggctcgc caccgcggtt
     8701 ggcacgaagc ttcagcgggc catcgtaagg ggtagtgaac gcattctggt ggccgaggac
     8761 gatgaattgg tgcgcgccgt cgcggtgagg atcttggagc gcgccggcta ccgcgtcacc
     8821 gccgccgagg acggcgatgc cgcctgcgtc gccgtgggta aagacaccta cgatctcgtc
     8881 attctcgacg tcgtcatgcc cggagccccc tgccgagagg tgatgcagcg cattcgggaa
     8941 atcagccctc gcaccgcgat tctcctgtcc agtggctaca ctgcgggtga gaagatcacg
     9001 agcttggtgc aggagacgaa gctcgatttg ctgcgaaagc cttacgatcc tgacactatg
     9061 ttggtggcgg tgagggccgc gttgagttta ggacgaggtc tggtgcagta attcaacgcc
     9121 gctttgctcg gacgggttga tacgtgtcac cccaccgctc atcggggccc cgcccgcgag
     9181 caacagcagc taaaggagcc tccgtgattc ggcatcccga aagacgcgag gcgcatgaga
     9241 ctcactgaag cggcatcccg ggaaggggtt gacgaaagcc cgcgcggagt cttcgtgacg
     9301 gcacgcggcg cgctctcgtc gctcggggcg aatgccgcag agcatctgat cgggtatcgg
     9361 gacggctccc tgggtcttca ccctctacag gtcggaagct accgggttcc gctcggcgcg
     9421 ttgagtgcct ccgcccgagc ccgggtatcc gaactggtcg gagcgcaccc gcagtatgcg
     9481 cggctcgatc cttcggtgtt gttcgcgatc ctagccaccc gcgaggcctt ggcgaccaaa
     9541 ccagaactcg agagcttcgc ggtgtgcctg ggctcctccc gtggagcgac cgagcgcctc
     9601 gaacagacgc accgcgactt cctgaaccat ggagaagtga agccagctac ttcccccacg
     9661 accacactcg gcaatctcag cagcaacgtc gcgcgggacc ggttcgggcc gaacgtaagc
     9721 gtcgagctct cgagcgcctg cacgagttcg ttgcacgccg tgttgttcgg atatgcttac
     9781 ttgcgcgccg gccttggcgc ggcctgcctg gtgggcggcg ccgaggcacc actcaccccg
     9841 ttcacgctcg cacagctcga cgcactcggg ttgctcaatc gagatctcga aagtgctacc
     9901 ccctgccgcc ccttgagcgg cgcggcgagc gccacgcccg aatcgacgat gaatcgcatg
     9961 gtgttgggcg aaggtgcttg tgtcttgttg ctccagcgct tgccacgctc ggaagctgct
    10021 cggcgcggag ccctagccga gatcgtgggc atcggcagcg ccctggagaa gacgccgcac
    10081 ccagccagta tgtccgcgga cgggcaagcc ctgcagcaag ccatgcgtgc ggcgctggcc
    10141 gcgcaagtgg ctgcggtgga cgccgtggtc gtacacgccc ctggcaccgc gagaggcgac
    10201 aaggccgaac tcgctgcact cgcgaaggtg ttcggcagtc acgtgcctcc gctctgctcg
    10261 aacaagtggc agctcggcca taccttcggc gcctcgggcg cgtttgggat cgatttcgca
    10321 ctagcacttt tggcgggagc cgtgcccgcg acggcgccgt ttcccagcgc tctaagtcag
    10381 cccacgggcg cggtacgccg cgtgctcgtg aacggcgccg ggtttggtgg gacggcggtc
    10441 agcgtattgc tccaagctgt ggcgaactga gctggggaga actgagctgg ggagcggcgt
    10501 cttcaacgca aggactggtg cccggcgagg aaggtgaacg cggggcgctc gcgctgatcg
    10561 gcataaacag cagagtaacc gggcgtcaac atgagcagca tgaactgctc gaactcacgc
    10621 gcgtcgagcc cgaaatccgc gccgatcgcg gcgaacacgg cggcaatgtt catctggatt
    10681 cctttccgct cccgcagccg gaaatggagc cagaacgcgc gccgcaaagc cggacccgcg
    10741 ctcacgccct cctctgtcag gatgtgtagc gcgaccggga tccgctcgtc cccattgtgg
    10801 atgggccgcc cataccccgc aagcactcgc ctgtccgcga aaacagccgc gagccaagcc
    10861 tcgcgctccg cagagttctc gggtatttcg agctgcacgg cgtaggcgtc gcggagtgcg
    10921 ccgaaaccgt aacccacgct gttgctggcc gcgattgccg cgcacatcga gagcgacggt
    10981 ggcacgcgag ccgaacccat atatgccgca atgcggttgc accaaatacg cgcatcggcg
    11041 tatccggtac taatccatag gcgctcgaaa acacgggctt ggctcaccga gaactcacgt
    11101 ccggtcacgg agaacaacaa gtattgaacg aagctgaagt cgagacactc gtgatgtaga
    11161 tcgcgccccc acaccacgac cctccccccg agctccgcga tcccagccga cgtctcgagg
    11221 cgcggcccag atacgctcgc cggcgtcatc atggcaggct aatcccgact cgagtcatca
    11281 gctgttcgag gtcgaagcca cgagcctccg gcaatgggcc ctgatatcgg taaagctcgc
    11341 tgaaaaaggg gaatcccttc aggcccaaag cccgagcgtg ttgggcttcc cgcagagcgg
    11401 cctcgacgcg caagaccaag aagcttcgct cggcttcgtc ggcggtgaga ccggcaccgt
    11461 gaaacccgag cgcacacaag cccgtcaagt tgagcgcgcc acagcgggtc acgatttggc
    11521 gacgctgctc caagaagaac tccagcgcgc ggaatgcgcc cggaaactgg gcgatcaggt
    11581 tttgaccggc gcggaccacc cggtctgctc gttcctcgtc gatatacccg aaccccgggc
    11641 tgtgctcggg atcccccgtg tctcgatgag gggtgggagc aaacgccccg acgaagccag
    11701 cacaactcgg ggagccgtgg cctggaatgc tacgcagacg gtcgagggca tccagtagcg
    11761 cgggcgtgcc cgtgagcgga ttctcgtacc aggccgcggc cgtgatcagc gcggaggcga
    11821 gcgaggctcc cgccacgcaa gcgttgcgcg cggcttcgac cccctcgtcg aacaggcccg
    11881 gttccgagaa caggctcttc agatatgaat acagctgggc tttcatcgcg taactcccac
    11941 cgcgagaccc acgacacgat caccaatcgg aggcacagcc gcacggtcga tgttcacgtc
    12001 aatcagacaa ggcccgccct cgtcgagggc caaccacctg cgcgcgatgc gtccgaggtc
    12061 ggacaaagtg tcgattcgga tgccctccgc gccacaagct ttggcaagct gatcgaagcg
    12121 cacgggcgcg atctgatgtg cgaccgactc cgctccggcg agcttctgcc cgtgacgcac
    12181 catacccaag ctcgaatcat tcaatacgat ataagtgacc ggcaaattct gctccacggc
    12241 gacggtcaac tcgaggctcg acatgagcat cgagccatcg cccgtgatgc aaatcgtacg
    12301 tcgatgggtt gcgaccgtag cccctatcgc ggcgctgatt gcccaacaca tcgtggagag
    12361 tcccatcgcc actcgatagg tatctggggt cgagcgggcc aaattcttgg tggaccacga
    12421 gaagctgttg ccagcgtcga cgtgcaccgc cacatcgctc ggcaaaacca ggccgaccca
    12481 atgcattaga tccttcggat gcacggggcc gtccggcacg ctctgcacca tcatggccgg
    12541 cgtcggccgg cgcggtgggc gcatgagaac ggccgaggtc ggcggcagtc gctgtagcag
    12601 cgcatcgatc acgcgggcgg gattggcgat tacggcgcgc gcggacggaa gccggtgaac
    12661 gtgctccgcg tgtcggtcca cggcgtagac cggaacgtcg gggaacgcgc cccatgcatt
    12721 cgtcgagagc tcatcgaagg tcgccccgaa cgtcaccacc agatctgctc gatgaatggc
    12781 ctcacgcgcc tccgggtgcc ccgaaaatcc cacgactcct ttgtagcaag cagtcgtgtg
    12841 tgagacccaa cgtttaccag ccgggctact gacgagctgt gcgccgagcc ggaaagctac
    12901 ggtggcgagc tgaaacgcct tcgggcccgc gtcgtctccc acgtagagta caggacattt
    12961 cgccttcacg actcggttca agagctcatc gatcgcgtcg agatccgcga cctccggcgc
    13021 gcgcagcgcc tgagccaagc ccgagtcgga gagcgcgccc ggccctgcca aaacgtccga
    13081 cggaatcgat aggtgaacgg gtcccatcgg cgccgtcacc atct
//
LOCUS       DASTKU010000849        10686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1728857, whole genome shotgun sequence.
ACCESSION   DASTKU010000849 DASTKU010000000
VERSION     DASTKU010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1728857"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1247)
                     /locus_tag="VFQ61_29855"
     CDS             complement(183..1247)
                     /locus_tag="VFQ61_29855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958749.1"
                     /translation="MREGSEPNRVEIGLIGDSAQDPLLFARIRSLFSAQAVVVSREAA
                     LFDAKSVLQPERADTVYLWIRVSPQGTARVYLALRQGGGSARYLFREVRLESGLDEVG
                     SETLAQVTHSSAQALWLREQQTPQHEVVAALQEEAEVDRASGGSSVSGAVGTSSLGSS
                     SVSIRPVSLRPTEAKDAPRFRFGASAGYTGHASGAEGWFHEPGGSLNFEYRGQLSLRL
                     AARYLVPTEFDVMPARVRLTGASGEVRLGWVMGISLFRVRLEAGLGLLIASCRSSLIE
                     GQESQAESSTPQNFDRGYAVAAGSLELPLGATWLALGADLRAPFRKTSYEIGGQEGAV
                     LSSMMSPGATLEFGLWFDRL"
     gene            complement(1510..2463)
                     /locus_tag="VFQ61_29860"
     CDS             complement(1510..2463)
                     /locus_tag="VFQ61_29860"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9958750.1"
                     /translation="MSQPEYTDLVCKAHRQELNPDEERRLDELLQTSLEVRLMDHMLS
                     EFERESRMCPGDDMLLARIQERVLGAPTSARPPARARRLTMVLLPAAVLLVSTLAGAG
                     MWLGSGRFTQLTAIWSSNSATKSAPAAPTVHKSRVAHPKPASTPSTPAAPAAPDSAAP
                     PVEPLGEPLPTVTGSTGASTQSVHAPTGELRMNIPNPSSELFARANLLRREGRGADAA
                     DLYRQLLERYPRSREAGPARLALAKYLHAKDPARALIEYRALASSGGALRAEALWGIS
                     ESAERVGERALADRALNDLVREFPDSPYAEVARARISHGTP"
     gene            complement(2460..3098)
                     /locus_tag="VFQ61_29865"
     CDS             complement(2460..3098)
                     /locus_tag="VFQ61_29865"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9958751.1"
                     /translation="MLRLVRVPGTNAPIRSVAEDELWPLAQAAIEGNVVALRTLLTAV
                     SPNILRVVRRVIGASHPDVEDVTQECAVEFVNALRRFRGESSVQHFASRVALRAAMNA
                     RRRQRAAKRGTPGPEPIEADETAGNHSPPDAIAASRASMQLARELCDELPAPQAEALA
                     LHCVLGYTMSEVASICGAPLETVRSRLRAAKSALMARALADPRLRELVEETA"
     gene            4368..5243
                     /locus_tag="VFQ61_29870"
     CDS             4368..5243
                     /locus_tag="VFQ61_29870"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HET9958752.1"
                     /translation="MPMTLIEGHYEVIGAAPDGDSVRFYPRERADWELVRSSRPIRSN
                     QRGGAQLRLDGVDALETHYSAMGHELGVLHQPLHEAQSAAANLLQWIGFRRVERDAKE
                     KVVSAQPARTPGYILTRFADAYGRCVAFAFEGKHPGKTGDQVYLDRSLLEESINYRLL
                     SEGLAYPTYYSKLYFELRRAMTRAVVEAREAGRGIWERDRTTAGWMVAATLEQVQEEA
                     FVLPKLFRRLVDYFALGAGDTSLAGFPEYLAQRDDRVFVLSEGQITNFDTVVETRGQT
                     LRLLTSVEDLVFQEK"
     gene            5470..5739
                     /locus_tag="VFQ61_29875"
     CDS             5470..5739
                     /locus_tag="VFQ61_29875"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9958753.1"
                     /translation="MTDRQVERITSALAEPRRFRILKELASRNGEMSCAQIVDTHGVS
                     NATISHHLKELETVELISIVREGKCNTIVLRRDVWKAYLARLAEV"
     gene            5897..6646
                     /locus_tag="VFQ61_29880"
     CDS             5897..6646
                     /locus_tag="VFQ61_29880"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9958754.1"
                     /translation="MKKLAGKVAIVTGASKGIGAGIARALGAAGAAVVVNYTSSKAGA
                     DAVVAEIVSQDGRALAVQADVSKPADVERLFAETRREFGAPSILVNNAGVYQFAPIDQ
                     VTTSEYRREFDINVLAPLLTTQEAVKHFGPDGGSIINVSSVVAWDAQPGGVVYAATKA
                     ALDSVTRVLSRELGPRKIRINSINPGLVETEGTIALGAIGSDFEKNAIASTPLGRIGR
                     PDDIAPVAVFLASDEARWVTGESLRIAGGYR"
     gene            complement(6637..8799)
                     /gene="scpA"
                     /locus_tag="VFQ61_29885"
     CDS             complement(6637..8799)
                     /gene="scpA"
                     /locus_tag="VFQ61_29885"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HET9958755.1"
                     /translation="MNEPILSPLQRWAELAKKEVRSSNLDALVWKAPEGIEIKPLYSR
                     ADLEGLPFVDSMPGMFPFVRGPRATMYAGRPWTIRQYAGFSTASESNAFYRAALASGQ
                     KGLSVAFDLPTHRGYDSDHPRVAGDVGKAGVAIDSVEDMKVLFDGIPLGEMSVSMTMN
                     GAVLPILASYIVAAEEQGVKPDQLSGTIQNDILKEFMVRNTYIYPPAPSMRIVADVIE
                     YTSRYMPRYNSISISGYHMHEAGATAEQELAFTLADGLEYVRAACRKGLHVDEFAPRL
                     SFFFAIGMNFFMEIAKLRAARLLWATLMKKHFEPSRPDSMMLRVHCQTSGVSLTEQEP
                     HNNIVRTSLEALAAVLGGTQSLHTNSFDEAIALPSEAAARVARNTQLVIEHESQVRHV
                     VDPFGGSYFMESLTAELAHRAQRLIDEIEGVGGMTRAIESSLPKLRIEAAAAERQARI
                     DRGLDVIVGVNRFQSEARAAVDVRIVDNAAVREEQLSRLAELRRSRDERATSAALDAL
                     REVAGRDDGNLLEVAVVAARARATVGEISAALEDVFGRHRSETKLVSGVYGHAYAQEP
                     AWQTVRGEVEQFTQAHGRRPRMLVVKLGQDGHDRGAKLIATAFADLGFDIDVGPLFQT
                     PDEAARQAVDNDVHVIGVSTQAAGHRTLVPALQAALRELSAQDIYVVCGGVIPPLDRD
                     ELLRAGVGAVFGPGTPVLEAARAVLALLRGRVPPANYR"
     gene            complement(8815..9834)
                     /locus_tag="VFQ61_29890"
     CDS             complement(8815..9834)
                     /locus_tag="VFQ61_29890"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HET9958756.1"
                     /translation="MDPVILNETESEQRPTLDLPVLDLAAFEAGTAEQRREIAAGFDL
                     GMQTIGFGVVAGHGIAPSLIERMFSTTSHFFELPNSDKAQVRSRVADGQFCGWVPFGA
                     DAASRVYGAHDDTPEDLRERFRAVVSDCAEVREKVGPNQWPTQPGNLAQVWTEYYRAV
                     ENMSLRVMQVSAVALGLEKHFFDPYFERHFSVLMATNSPPFTGPRKPGQARCGTHTDT
                     GTMTFVYQRDTRGGLEVQVGGKWLLPAPPAGSFIVNCGDLLARWTNNRWKSTVHRVGG
                     PGAAIDQRRVSVVFFHQPALDIPLESLPTCVDAEHPAQFDPITLREHFSNQQRRLKTR
                     DDVNN"
     gene            complement(9847..>10686)
                     /locus_tag="VFQ61_29895"
     CDS             complement(9847..>10686)
                     /locus_tag="VFQ61_29895"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9958757.1"
                     /translation="TALRLIVALAAFVIFYIVSGRRSVRWDPRLWASALSLGVLGTAL
                     PMLGIISALQQISSGVVAIIATCGPAFAVVFAHWWLPDERITPRRLLGLSITMLGAVL
                     LATRNETGLTDVGRGVSSAYLVLVVSLLGSHASIVLSRRYLSKYDMFDVVSLQMLAAA
                     VVLAPIALTHLDVGRVSNPGLAWFSVLFGGIVGTFLAFSSRFRIIKDFGATDAATIDY
                     LVPLIAVAFGALFARERVTPVILSSMLLILSGIRMIRVPVRRGAPQSVAALKPDEALR
                     RQV"
BASE COUNT         2030 a         3388 c         3389 g         1879 t
ORIGIN      
        1 gcgccgttgc tcgcggccgc acggccaccc gagctggtgc caccgctgcc ctctccggta
       61 tcacccgccg aagtgcggtc cagcgtggcc acgacgatgt gatctctggc gcaagccccg
      121 cagagagtgc tcgtcaaaaa cagagtcgtt gcgagaatag ataggcgcat cggtcgttca
      181 cctcacaagc gatcgaacca caacccaaac tctagggttg ctccggggct catcattgag
      241 ctcaacaccg cgccctcttg ccccccaatt tcgtacgatg tcttgcgaaa gggcgcacgc
      301 agatcggcac cgagagccag ccaggttgcg ccaagaggta actccaacga ccccgccgca
      361 accgcgtaac cgcggtcgaa attctgcggc gtactcgact ccgcctgtga ttcttggccc
      421 tcgattaacg agctcctgca gctggcgatg agcaagccca ggccggcttc gagccgcacc
      481 cggaatagcg aaatccccat cacccagccc aagcggacct ccccgctcgc gccggtcaag
      541 cgcactcgcg ccggcatcac gtcgaactcg gtgggaacca agtagcgagc cgccaagcgc
      601 aacgacagct ggccacggta ttcgaaattc aaacttccgc cgggctcgtg aaaccagcct
      661 tcggcacccg aggcgtgccc cgtataccca gcagaagctc caaaccgaaa ccgcggtgcg
      721 tctttggcct cggtcgggcg gagcgaaacc gggcgtatgg aaacggacga actcccgagc
      781 gacgaggttc ccacggcccc ggatacactc gaaccccccg acgctcgatc tacttccgcc
      841 tcctcttgga gtgccgccac tacctcgtgc tggggagtct gctgttcacg caaccacaac
      901 gcctgcgccg acgagtgcgt gacctgcgct agcgtctcac tacccacctc gtcgagccca
      961 ctttccaggc ggacctcacg gaacaagtag cgtgcgcttc cgccgccttg ccgcaacgcg
     1021 aggtagaccc gcgccgtgcc ctgcgggctc acgcggatcc acagatatac cgtgtccgcc
     1081 cgctcgggtt gcagcacgga cttagcgtcg aacagcgcgg cttcgcgcga gacgaccacc
     1141 gcctgcgccg aaaacaaact ccgaatgcgt gcgaacagca gcgggtcttg cgccgagtcg
     1201 ccaatcaaac cgatctccac ccggtttggc tccgacccct cgcgcaagct cgcttctttc
     1261 gagtgtgagc ttggggtggc cgtgcgggca gcgcctgctt ccgagctgcc ctcgagctgc
     1321 gtatcgattg gacgcgcgcg cgtcgtcgtc gcgccagcag ggtgagtgat agaggagcgt
     1381 ggggatcccg aattcggcgc cgccgcacag ggcatcggcg cgccgccgca ccaaacgccg
     1441 atcgacaagg cgcacgacca acgcaaagcc gaagctctga aattcctaaa tccagccgca
     1501 ggccacgcct cagggcgtac catgcgagat ccgcgcgcgc gcgacttctg catatggtga
     1561 atcagggaac tcacgcacca aatcgttcaa ggctcgatcg gccagagcgc gttctccaac
     1621 tcgctcggcc gactccgaga taccccacaa agcttccgca cgcaaagcgc cgccactgct
     1681 cgccagcgct cggtactcga tcaaggccct cgcggggtcc ttggcatgca gatacttcgc
     1741 gagcgccaga cgggccgggc ccgcctcacg cgaacgcgga tagcgctcca gcaattgccg
     1801 gtacaaatca gccgcgtccg cgccgcgccc ttcacgacgc aacaagttgg ctcgcgcaaa
     1861 cagctcgctc gaagggttgg ggatgttcat tcgcaactca cccgttggag catgaacgga
     1921 ctgggtgctc gccccagtcg agcccgtcac ggtgggcagc ggttccccga gcggttccac
     1981 tggaggcgct gccgaatccg gcgccgccgg ggccgctggt gtcgacggcg tgctggcggg
     2041 cttggggtga gcgactctgg atttgtgaac cgtcggcgcc gccggggcgg acttggtcgc
     2101 cgaattcgag gaccaaatcg ctgtcaattg cgtgaaacgc ccactgccca gccacattcc
     2161 ggctcctgct agcgtggaca ccaacagcac cgctgcgggc agcagaacca tggtgaggcg
     2221 acgtgcacgc gcgggaggcc gagccgaagt tggcgcgccg agcacccgtt cttgaatccg
     2281 agcgagcaac atgtcgtccc cggggcacat gcgactctct cgttcgaact cggacaacat
     2341 gtggtccatc aaacggacct cgagcgacgt ttgtagcaac tcgtcgaggc gacgctcctc
     2401 gtccggattc aactcctgac gatgcgcctt gcagaccaga tccgtgtatt ccggctggct
     2461 catgcggtct cctccaccag ctcacgcaaa cgcggatcgg cgagagctcg cgccatcagc
     2521 gcggactttg ccgcgcgcag gcggctgcgt acggtctcga gcggcgcgcc acaaatcgac
     2581 gctacttcac tcatcgtgta tccgagcacg caatgcaaag cgagggcttc cgcctgtggc
     2641 gccggcaact cgtcacacag ctcgcgtgcc agctgcatgc tcgcgcgtga agctgcgatt
     2701 gcgtccggcg gcgagtgatt tcccgccgtc tcgtccgcct cgatcggctc tggaccgggc
     2761 gtaccgcgct tggctgcgcg ctgtcgccgc cgcgcgttca tcgccgcccg cagcgccact
     2821 cgcgaggcga agtgctgaac cgaagattcg ccgcgaaatc gccgtagcgc gttcacgaac
     2881 tcgacggcac actcctgcgt tacgtcctcc acgtccgggt gtgacgcgcc gatgacgcgc
     2941 ctgaccaccc gcagaatatt ggggcttacg gcggtgagca gagtccgaag ggctaccacg
     3001 ttcccctcga tcgctgcttg agccaaaggc catagctcgt cctccgccac cgaccgaatc
     3061 ggagcgttag tgccggggac acggaccaag cgtaacatcc agcctttctg tgttacctct
     3121 ggcaaaatcg ggttagcgac tcaaagcgat ttagatcgaa ttgttcagtt cggtcgttcg
     3181 gtactgctgc gattccgaac cgaaacaaag acggagcgca cccagatccc aatcgggttc
     3241 cagctgcgaa gcttggtcat tcacgagaac ggggcacttc tttgggagag attgcggggc
     3301 agcctcgcgc cgccggcacc gaaagcacgg gttcaagtgt gagccagtgc aaaggggcga
     3361 cgactggtgc gtgggaagtc cggcatcttt ggggcatccg gccatcgaat aaagagcgcg
     3421 aagctgcccg cggagagccc atggcaccgt ctcagtccac gatccaattc gatacaccgc
     3481 gccggccccc tcaataacgg ggctcatcca gcgcttcgcc aattttatgg ctccagaacg
     3541 atcaaccgtc tgagcgggat tcgggcgaaa ttgcggctga gtcttggctg ggctgccgcg
     3601 cgcccccccc ctcattccca aggttggcca agcgagcggt ggccgcgccg gcaccgggat
     3661 tgcgagtggc gcgggcggtc aagtttccgc cgtggcgggg gcgcacaaag cgtggaacgc
     3721 agagctccgg cgggggcgcg attgccaacc gagagcgtca acgagctcga acggagtcat
     3781 cgggcggcgc cgcgagtagc gccggatgaa cggcggggac agaacggcac gactagcggt
     3841 ggagacgagg cgccgcaaat cgtgaccact ctcgacgcct tcaagaaagg ccatcagcgg
     3901 taccgcgccc ggggcaattg aacgtcactt ggcaccacaa ctgctgggca gacaccgtga
     3961 atcagcgcat gccgcgcacc caccgcggcc agatccacgg gtcgagaatc tgttcacctc
     4021 gtgcggcaat tcgtcctcca tgaatgcggg tttcgaaacc catctatcgg tctaaacagc
     4081 gtctcattct cgtcataccc gagtagacac agcggttgga ccctccaacc tgcaaggatg
     4141 cggggcctta tgctaccagc gacgaagcgt gcgggcgcag agccgcgtgc gagggctggt
     4201 ccaacgttag cctatacgac gctgaatgat tggcgatctc tcgattctcc gcggactcat
     4261 catcaggcgg tacaggagta tcgtacacgc aatggccaag gcgtgagccg gagcacctgt
     4321 tacgttttac tcgcgcgtgc gttgcgcgtc caaacgaggg ttttgccatg ccgatgactt
     4381 tgatcgaggg acattacgag gtgatcgggg cggcgccaga tggggattcg gttcgattct
     4441 atccccgtga gcgcgccgat tgggagctgg tacgatcgtc gcgacccatt cgctcgaatc
     4501 aaagaggagg ggcccagctg cgtctggatg gcgtggacgc cttggagacg cactactcgg
     4561 cgatggggca cgagttggga gtgctgcatc agccgttgca cgaggcgcag agcgcggccg
     4621 cgaatctgct gcagtggatc ggctttcggc gcgtggagcg agacgcgaag gagaaggtcg
     4681 tgagcgccca gcccgcccgc actccgggct acattctcac gcgcttcgcg gacgcctatg
     4741 gtcgttgcgt cgccttcgct ttcgaaggca agcacccggg taagacgggc gaccaagtct
     4801 atctcgatcg gtctttgctc gaggagagca tcaattatcg actcttgagt gaggggctgg
     4861 cttatccgac atactactcg aagctgtact tcgagcttcg ccgcgcgatg acgcgagccg
     4921 tggtggaagc gcgggaggcc ggtcgcggga tctgggagcg cgatcgcaca accgcgggct
     4981 ggatggttgc cgctaccctc gaacaagtgc aggaggaagc gttcgtgttg ccgaagctgt
     5041 tccgtcgact cgtggactac ttcgcgctgg gtgcgggcga cacctcactc gcgggttttc
     5101 ccgagtattt ggcgcagcga gatgaccggg tgttcgtgct ttcggagggg cagattacca
     5161 acttcgacac agtagtggag acacgcggcc agacgctgcg gctgctcacg tccgtggagg
     5221 acctggtctt ccaagagaag tgatgttttt aggctttagc ccggtcgcac tcagcgggtg
     5281 ccctcttcac gctcggagct gagggggccg gcgcggtggg gagggtaggg ctgggtgcca
     5341 agcgggctgc gctggtggga aagacggagc ggcttcgcgc tcgttcgcag tgtggccgcc
     5401 gcccacggct tctccggcga ggcggctggc gcggtttttg gtcaaaggct atacagctat
     5461 gtggtttcgt tgaccgaccg acaagtggag cgcattacga gcgcgctcgc tgagcctcgg
     5521 cgttttcgca tcctcaaaga gctggcttcg cgcaacgggg agatgtcgtg cgcccaaatc
     5581 gtggacacgc acggggtgag caacgctacg atttcgcatc acctcaagga gctcgaaacc
     5641 gtggagctga tctcgatcgt acgcgaaggc aaatgcaata ccatcgtcct gaggcgggat
     5701 gtgtggaagg cgtacttggc gcggctcgcc gaggtttgag ttatttcggc gtgcgcgcgc
     5761 ttgacgcatc cagaacccat acttagatga gcgtctacac gacgccgccg catggagctc
     5821 gcgggcctac gctgcggctt ttgtttcgat catcatttag acagttgtcg aaatgtcgaa
     5881 gtatggagaa gttcacatga aaaagctggc tggtaaggtc gcgatcgtga cgggagcatc
     5941 caagggcatc ggcgcaggca tcgcgagggc tttgggcgcg gcgggcgccg cagtcgtcgt
     6001 gaactacacg tccagcaagg cgggcgcgga cgcagtagtt gcagaaatcg tgagccagga
     6061 tgggcgagcg ctggccgtgc aggcggatgt ctcgaagccg gctgacgtgg agcgcttgtt
     6121 tgcggagact cgtcgcgagt tcggcgcacc cagtatcttg gtcaacaacg caggcgtgta
     6181 tcagttcgcg ccaatcgatc aagtgacgac gagcgagtac cggcgcgagt tcgacatcaa
     6241 cgtgctcgct cctttgctca ccacgcagga ggcggtgaag cacttcgggc ctgatggcgg
     6301 aagtatcatc aacgtgagtt cggtggtggc gtgggacgcg caaccgggtg gcgtggtgta
     6361 cgccgcgacc aaagccgcgc tcgattcggt gacacgggtc ttgtcgcgtg agctcggccc
     6421 gcgcaagatt cgaatcaatt ccatcaatcc tggcctggtc gagaccgagg ggaccattgc
     6481 gctgggcgcg atcggaagtg acttcgagaa gaacgcgatt gcgagcacgc ccctcgggcg
     6541 aatcgggcga cccgatgaca ttgcgcccgt cgccgtgttc ttggcctcgg acgaggcgcg
     6601 ttgggtaacc ggagagagcc tacgcatcgc cggcggttat cgatagttcg ccggcgggac
     6661 gcgtccgcga agcaaggcga gtaccgcacg cgcggcctcg agcacgggcg tgccgggccc
     6721 gaataccgcg cccacccccg cccgcagtaa ctcgtcacgg tccagtggtg gaatgacgcc
     6781 cccgcacacc acgtagatat cttgggcgga tagctcccgc agcgccgctt gtagcgccgg
     6841 caccagcgtg cgatggcccg cggcttgggt cgacacgccg atcacgtgaa cgtcgttgtc
     6901 gacggcttgc cgcgcggcct cgtccggcgt ttgaaagagt ggacccacgt cgatatcgaa
     6961 gccgagatcg gcaaatgcgg tggcgatcag cttggccccg cgatcatggc cgtcctgtcc
     7021 gagcttgacc acgagcatgc gcgggcggcg gccgtgagct tgggtgaact gttcgacttc
     7081 accccgaacc gtttgccaag cgggttcttg ggcataagcg tggccgtaca cgccactgac
     7141 gagcttggtc tccgaacggt ggcgcccaaa tacgtcctct agcgcggccg agatttctcc
     7201 cacggtggcc cgcgcgcgcg ccgccaccac cgcgacctcg agcaggttgc cgtcatcgcg
     7261 accggcgact tcacggagcg catccagcgc ggcgctggtg gcgcgttcgt ctcggctgcg
     7321 tcgaagctcg gccaagcgcg agagctgttc ttcacgaaca gccgcgttgt ccacgatccg
     7381 gacatcgacg gccgcgcgcg cctcggactg gaaacggttc acgccgacaa tcacgtccag
     7441 cccgcggtcg atacgggctt gacgctcggc ggccgcggcc tcgattcgca gcttcggtaa
     7501 gctagactcg atggcgcggg tcatgccgcc caccccctct atttcgtcga ttagccgttg
     7561 ggcgcggtgg gcgagctccg ccgtcagcga ctccatgaaa tacgagccac cgaacgggtc
     7621 caccacgtgc ctcacttggc tctcgtgctc gatcacgagt tgcgtgttgc gcgccacgcg
     7681 tgccgccgct tcgctcggta aggcgattgc ctcgtcgaag gaattggtgt gcaggctctg
     7741 ggtgcccccg agcaccgccg ccagcgcttc gagtgaggtt ctgacgatgt tgttgtgcgg
     7801 ctcttgttcg gtcaggctca cgcccgaggt ttgacaatgc acacggagca tcatcgagtc
     7861 cggccgggac ggctcgaagt gctttttcat gagcgtcgcc cagagcaacc gcgcggccct
     7921 gagtttggcg atctccatga agaagttcat gccgatcgcg aaaaagaagg agaggcgagg
     7981 ggcaaactcg tcgacgtgga gccccttccg gcatgcggcg cgcacgtact cgagcccgtc
     8041 cgccaaagtg aacgcgagct cttgttcggc cgtcgcgccc gcctcgtgca tgtggtaccc
     8101 ggagatcgag atcgagttgt agcgcggcat gtaccggctc gtgtactcga ttacatccgc
     8161 cacgatgcgc atggagggcg caggagggta gatatacgtg ttacggacca tgaactcttt
     8221 cagaatatca ttctgaatgg tgcccgagag ttgatcgggt tttacgcctt gctcctcggc
     8281 ggccacgatg tagctcgcca agataggcag gacggccccg ttcatggtca tggacacgct
     8341 catctctccg agcgggatgc cgtcgaacaa gaccttcatg tcctcgaccg agtcgatggc
     8401 cacgcccgcc ttgcccacgt cacccgccac gcgaggatgg tcgctgtcgt acccgcggtg
     8461 ggtaggtaaa tcgaaggcga cgctcagccc cttttggcca gaggcgagcg ccgcccgata
     8521 gaaagcattg gactcgctgg cggtggagaa gccggcgtac tgccgtatcg tccaaggtcg
     8581 gcccgcgtac atggtggcgc gcgggccccg gacgaacggg aacatacctg gcatcgagtc
     8641 tacgaagggg aggccctcca agtcggcacg gctatagagc ggcttgatct cgattccctc
     8701 gggcgccttc cagaccaaag cgtctaggtt cgagctgcgg acttcctttt tggcgagctc
     8761 ggcccagcgc tggagaggac tcagaatcgg ctcgttcatt gggcggcatg agcctcagtt
     8821 gttcacgtca tctcgagtct tgagccgacg ttgctgattc gaaaagtgtt ctcgtagagt
     8881 aatcggatcg aactgcgcgg ggtgctccgc gtcgacacac gtcggcaacg actcgagcgg
     8941 aatgtccagc gcaggctggt gaaagaacac gaccgacacg cgccgttggt cgatggcggc
     9001 acccggacca cccacccggt gaacggtcga cttccagcga ttgttggtcc aacgggccag
     9061 gagatcgccg caattcacga tgaagcttcc cgctggaggc gccggcaaca gccacttgcc
     9121 accgacctgc acctcgagcc ctccgcgcgt atcccgctga tatacgaagg tcatggtgcc
     9181 cgtatccgtg tgcgtgccac agcgggcttg cccgggcttg cgaggccccg tgaaaggggg
     9241 agagttggtg gccatgagca cgctgaagtg gcgctcgaaa taaggatcga agaagtgctt
     9301 ctcgagcccg agggccaccg ccgagacctg catcacgcgc aggctcatgt tctcgaccgc
     9361 tcggtagtac tcggtccaga cctgggcgag attgccgggc tgggtcggcc actgattggg
     9421 gccaaccttt tcccggacct ccgcgcagtc gctcactacg gcgcggaagc gctcccgcag
     9481 gtcctccggg gtatcatcgt gtgcaccgta gactcgcgag gccgcgtctg cgccaaatgg
     9541 aacccatccg caaaactggc catccgccac gcgggagcgc acctgcgctt tgtcggaatt
     9601 gggcagctcg aagaagtgcg aggtggtgga aaacattcgc tcgatcagcg acggcgcgat
     9661 gccatggccc gcgaccacgc caaaaccgat cgtctgcatt cctaagtcga accccgccgc
     9721 gatttcgcgc cgttgctccg cagtgcccgc ttcgaaggcg gccaagtcca acactggtag
     9781 gtccagggtg gggcgttgct cggactcggt ctcattcaat attactggat ccatcgaatc
     9841 tcctcctcaa acctgtcggc gtagggcttc gtccggcttg agcgcagcca cggactgagg
     9901 tgctcctcgc ctgaccggaa cccggatcat ccgaatgccg gaaagaatca gtaacatgct
     9961 ggataggatg actggcgtga ctcgctctct agcaaacaag gctccgaacg ccaccgcaat
    10021 caatggcacg aggtagtcga tcgtcgccgc gtcggtggct ccgaaatcct tgatgatgcg
    10081 gaagcgtgag ctgaacgcca ggaacgttcc cacgattcct ccgaacagca cactgaacca
    10141 cgcaagtccg ggattcgaga cacgccctac gtctagatgc gtgagcgcaa tcggtgcgag
    10201 taccaccgcg gcggccagca tttgcagact gaccacgtcg aacatatcgt atttgctcaa
    10261 gtaccgacgc gaaagcacga tcgacgcgtg gctcccgagt aaagagacga ccagcacgag
    10321 gtatgcggac gataccccgc gcccgacgtc ggtcagcccg gtctcgttcc gggtcgcgag
    10381 taggaccgcg ccgagcatcg tgatcgaaag gccgagcagg cgccgcggag tgatgcgctc
    10441 gtccggtaac caccagtggg cgaacacgac cgcgaacgca ggcccgcagg tggcgatgat
    10501 agcgaccaca ccactcgaga tctgctggag agcgctgatg atgcccagca tcggcaaagc
    10561 cgtgccgagc acacccagcg agagggcgga tgcccaaagc cgggggtccc agcgcacact
    10621 ccgccgccca gacacgatgt agaatatgac gaacgcggcc aacgctacga tcagccggag
    10681 cgcagt
//
LOCUS       DASTKU010000850        15449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_611902, whole genome shotgun sequence.
ACCESSION   DASTKU010000850 DASTKU010000000
VERSION     DASTKU010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 15449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15449
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611902"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VFQ61_29900"
     CDS             <1..683
                     /locus_tag="VFQ61_29900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HET9958758.1"
                     /translation="IYHDSALEGVVYTFQELKTAIDPSLPVVSDSSLQPVCEEIRRNR
                     QAIELVRELAEKRRVPLNVDILKKIYVTLHPEEGDVKTVKYRKDIPQHRLYFHEYAAP
                     DKIAYKVRQVIDWLNGPEAKKMKNPLRVAARVHFDLLRAFPFQSDSGKVSRLFMNVIL
                     MRAGYPPTIIHSTERQKYYEALKGQLPIIITMTNEAINNALLSIEKRLDEYEARGRVG
                     VAPPPAAS"
     gene            complement(778..2259)
                     /locus_tag="VFQ61_29905"
     CDS             complement(778..2259)
                     /locus_tag="VFQ61_29905"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9958759.1"
                     /translation="MTYRSAGRLFRWMAASTVSSLVWQTALAQERPASAAGNATSSAE
                     DATTAAPSAAAPNEAESGVLQRRKTGVGLRRCLELAERNYPKVHEARARLEQKRAQSF
                     QARTAPFSEFTLSFAFAPAPVVQGTALYSPSSDLPITSKMSVGWQGSLTGLVPLWTFG
                     KITNLWDAADAQVKVGEQEIRKEKNDLKLQVRRAFYGVLLTRDALTLVDEALSRVDKY
                     AAHLAERLKEGEGDEIDLWRLQIQREDLVVRQSEARKQEQIAMAGLRFFTGVVETATF
                     DVPDRPLQRVNHHLAPLARYLSAARLYRPEVNMARAGVLAREAQLRQQRAGFFPDIGL
                     ALGATIVNAPYVTDQRNPFVRDVGHAQSYGASLAVRYKLDFLPQSARYAQARAQLEEQ
                     RATERFALGGVGVEVEQAYREAEDAERRLDALTRAVGFAKKWMIAVQQGIDVGTFEDA
                     DIVDPAKEYALRRFGQMSATFDYNVAVGKLAQATGWDVIAADD"
     gene            complement(2256..3737)
                     /locus_tag="VFQ61_29910"
     CDS             complement(2256..3737)
                     /locus_tag="VFQ61_29910"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9958760.1"
                     /translation="MTLTLRARSGEFGEVRLEDTPPRVLIVDDEPMLRRSLARLLLSR
                     GMGVVTADDGAVAMDVLRREPVDVALVDLMMPRVGGLELLDHVRAEHSGVEVVIMTAF
                     ADVETAVKAVRAGAYHFLTKPFRNNDEVVVTITKAAERRRLVDRAAMLERRLEHLEKF
                     GELIGNSTEMQEVYRLAVGVAPTSSTVLILGESGTGKELTARAIHQHSPRANNPFIPV
                     NCSAIPVDLVESELFGHVRGAFTGATATRPGLFEAADRGTLFLDEVGDLPPIAQVKLL
                     RALQEGEVKRVGSNETRIVDVRVIAATNVDLKGRIQAGRFREDLYYRLNVVAISLPPL
                     RERKEDIPLLAYHFLQKYAARSNSPVRKISPDAMAVLQNQRWSGNVRELENAIEHAVV
                     FCRDTTIAPADLPFTRKQSTAAEPLIQPTPDPSGFSAPLADLPYREAKDRAVHAFETA
                     YFNALLQRAGGNVSEAARQAGLDRSNFRRAAKRSGVRMRDDDA"
     gene            complement(3734..4774)
                     /locus_tag="VFQ61_29915"
     CDS             complement(3734..4774)
                     /locus_tag="VFQ61_29915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9958761.1"
                     /translation="MRGSTVVVAEDDRLTREALSSVLRAHGYVVEAFGDGQAAVERIA
                     RGGVDVVLLDIVMPKLSGLEACRLIKGMTHDSFLPVLLLTVKTDSASRVEGLKIGADD
                     YVCKPFDERELTARVEAMLRIKKLHDHVAEARRRLEQLSVHDELTGLYNYRYLHPRMN
                     EEFKRAERYHDPLACLVIDIDRLESHNQAGGRRHGDRVICGVAEALRRCVREVDVVAR
                     FGGDEFLVLLPSTHFAGSVTVAERIWRDVSETPIQGDLPEPVRASLSVGVALFPSRDV
                     RTKDQLLKAADTALHNAKREGKNRVCVYQQQGQLYTPVSGSTQGDATTSGATAPRARL
                     PSESEAQRRGIE"
     gene            complement(4938..6797)
                     /locus_tag="VFQ61_29920"
     CDS             complement(4938..6797)
                     /locus_tag="VFQ61_29920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958762.1"
                     /translation="MSPPVLSRGHRALLACLVACGLSGVGSRAFAQAAKPAPPREHEV
                     KFQARHVEVDGDGESLRLSGDVVVRAERFQITSSDIRLSRSRRGLHVVGQGKLSFCPC
                     PEPPVTLGFASADFAPPTDILLYGGTLRLLGLPLFWSPFLWLRAADRVGLLPPNFAYR
                     GEEGFVFGSGFHLPLGPLGRNPSRFDLRAWGYSRGGFGSSIEVQTESGFSRVGWDYLE
                     RANSLDISSQWAARSESGAVWAERVEVLRGTRAQIAPATLERVALPKDRVRLAVGSAG
                     QHGFAVTLEADARRGRALDDFGAQGGAWMGPGFTLGLGTSLGKSAQLGFHAETRSAWA
                     ANGALAVGNLGWGASVSVPIAALLWDIELRQRLSWAANETDAASASRSALRSRLGLPL
                     LRRSESSTTWLEPYAELSTLVARLEEPIWFAADLRSGFFASAITGAKIAFSQAGAGAR
                     RALEWSLGAGGAVRGKSELTPLLASELSAAWGSVRASGLLAGDPKTRALETSVRAELG
                     EVDGPRLGLRLDARNSARVLLSTLESDLALWGRSLSMQGTSLRARLGSPLLLGFAASV
                     GSDFDLDTRRALASAAELAYRHRCKCVSFHLGGSRREGRRGFDVNVGLELFPR"
     gene            complement(6827..8065)
                     /locus_tag="VFQ61_29925"
     CDS             complement(6827..8065)
                     /locus_tag="VFQ61_29925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958763.1"
                     /translation="MTDMLVRWFGGLLCAFTLSAPQTAQAQAADTGAAPAGFAQPGLA
                     QTQAQRTPTGVAADHGVGRPVVPASEPAPPREAVSRFHDSLLVLDQSITTQTVGVGSQ
                     YLSDNPTYDITLRLWPRFYLVENERRDLSLRLDFRLAREFTNSDTTTRRGEWAPYDTE
                     LWLAWFEHLRRVENQRTSVLIRFPYLVVPTSKASAGSGRILGVGIGTGIDQEVPLRGP
                     GAYFLPKGKVSARINYSYHFARSNVATNDTVQRVRVDPEGRSAPSDQLSGSALPQHVV
                     LGSLRFETALVEKLGWSADFGFRYDRRYALTDDVDVCNLGTGCVAPIRLNDASRSTVR
                     TIFATELMYTFNDYLAASLGYINIAAQLGEDGQRRSLFYSPWARFYVSLGVTLDAAYR
                     AASGRRPPPAAAQVGPARAF"
     gene            8204..8566
                     /locus_tag="VFQ61_29930"
     CDS             8204..8566
                     /locus_tag="VFQ61_29930"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET9958764.1"
                     /translation="MPPALPATEITALLGRGTHFEGKLDFEGRVRIDGSFRGQIKTRD
                     VLIVGDGAEIEAEIEAGTVIVKGGTLSGNVHATEGIELYVPARVNGTLRAPEIFMDKG
                     VQFSGTCTIEPRTPTEPG"
     gene            8995..9594
                     /locus_tag="VFQ61_29935"
     CDS             8995..9594
                     /locus_tag="VFQ61_29935"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9958765.1"
                     /translation="MKQSGLSDRHAFGRILRAAALTATLSGAAAAQADARERPVERYD
                     VRTAGTSAGAARTVVRAGDGVVRSVVTDFSRYPDFISRFKTAKVIGKSGDKTDVYLQV
                     PILNGAVKIWAVVRFDPPKEVNGGQVVSGKMLKGNVKRLDAVWRIQPLDAESTELKLE
                     LTIVPDLPVPEAVVLPEVRYAAAKAVSGTRDEAERRATH"
     gene            10060..14610
                     /gene="gltB"
                     /locus_tag="VFQ61_29940"
     CDS             10060..14610
                     /gene="gltB"
                     /locus_tag="VFQ61_29940"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HET9958766.1"
                     /translation="MPLPPRRGLYDPRFEHDACGVGFVANIRGEASHDIVKKGVDILL
                     NLVHRGACGCDPLTGDGAGILLQLSHSFFKRESDRAGIDLPEPGMYGVGNVFLPRDKT
                     QRRRCQEILEDKISGTGQKLLGWRNIHVNEDALGPMARASVPVIRQVFVGATTTNQDE
                     FERKLFVIRKWAERTVRESNIASRGMFYMPSLSSRTIVYKGLLLAEQLTGFYRELEDP
                     EMKSALAMVHQRFSTNTFPTWELAQPFRMLAHNGEINTVRGNSAWMSAREQLIDAHAV
                     FGNDFRHILPTIAPGGSDSAQLDNVAELLLHAGRSLPHVMMMLVPEAWQNDPLMPEHK
                     RDFYEYHSCLIEPWDGPAALAFTDGKRIGGILDRNGLRPARWTITKDGLVVLASETGV
                     LDFAPENIERKGRLEPGRMFLVDTERGRIVEDEEIKDEICKRRPYGKWLRENKIALDE
                     IEDSTGSSNHVERTSLLDRQKIFGYSQEDLKMLMAPMAAQGEEAVGSMGTDTPLAVLS
                     DEPQLLFNYFKQHFAQVTNPAIDPIREEMVMSLKAYIGGEGSVLAELPDHAQMLELEH
                     PILTNADIAKLRETHLQQVRVPPTLPMLYPVAEGGAGLKEALDELCRQASLAVNDGYS
                     LLILSDRGATDALAPVPSLLATGAVHHHLMRNGTRMRIGIIVETGEAREVHHFCLLTG
                     YGAGAINPYLAFETIENMVREGTFPALKDAELAKKKYIKAINKGLLKVMSKMGISTLQ
                     SYHGAQIFEAIGLSPEVVDRYFTGTTSRISGIDLDVIAEESRRRHEVAFPKAKQQLPL
                     LPMGGQYHYRAQAERHLWNPTTVAKLQHAVRMEDVKSYKEYAAFINDARDGLITLRGM
                     WDLKSDRAPVPLEEVEPAAELVKRFATGAMSFGSISAEAHENLAIAMNRIGGRSNTGE
                     GGEREERFRPDSNGDLRRSSIKQVASGRFGVTTHYLVNADELQIKVAQGAKPGEGGQL
                     PGHKVDAIIAKTRHSTPGVTLISPPPHHDIYSIEDLAQLIFDLKMVNPRARISVKLVA
                     EAGVGTVAAGVAKAHADVILISGFDGGTGASPLTSIKHAGVPWEMGIAETHQVLVKND
                     LRSRVILQSDGQMRTGRDVIIAALLGAEEFGFATAPLVASGCIMMRKCHLNTCPVGIA
                     TQDPVLRAKFQGKPEHVIRFMFYVAEEAREWMAKLGFRTVRELVGHVECLEPRRDVQH
                     WKATRLDFSDILAPAEAAPGVAVCKTIEQDHGLDKALDIELLRLCGAGLERGEPVRAD
                     LPIRNVHRTVGAMLAGEVARLHGKAGLPEGTLRFGFNGSAGQSFGAFLTNGIEFSLEG
                     DANDYAGKGLAGGVIAVRPPRGASFRADESILIGNTALYGATSGRAFFNGRAGERFAV
                     RNSGATTVVEGVGDHGCEYMTGGRVLVLGRTGRNFAAGMSGGYAYVLDEAGDFEKRCN
                     LGMVELEALSAEDAAIVASLLEEHRQRTGSPKAISLLNNWESALSRFVKVVPVEYRRV
                     LEELARREKTAPSSSAVLHGSP"
     gene            14904..>15449
                     /locus_tag="VFQ61_29945"
     CDS             14904..>15449
                     /locus_tag="VFQ61_29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9958767.1"
                     /translation="MGKVTGFLEFKRAEPSKRPVDERVKDYREFELPVVNETLVAQGA
                     RCMDCGVPFCNSGCPLGNLIPDWNDHVFKNEPKLAITSLHSTNNFPEFTGRVCPAPCE
                     AACVLGINDDPVSIKLIERSISDRAFGEGLVQPELPRTQTGKKIAVIGSGPAGLAAAQ
                     QLARVGHSVTVFEKDDRIGGLL"
BASE COUNT         2978 a         4939 c         4859 g         2673 t
ORIGIN      
        1 ggatttacca cgacagcgcg ctcgaaggcg tcgtgtacac cttccaggag ttgaagacgg
       61 ccatcgatcc atcgctgccg gtggtatccg actcgagcct gcagcccgtg tgcgaagaaa
      121 tcaggcgcaa caggcaggca atcgagctcg ttcgtgagct cgcggagaag cgccgtgtgc
      181 cgctcaacgt cgatatcttg aagaagattt acgtcacgtt gcaccccgaa gagggcgacg
      241 tgaagacggt gaagtaccgc aaggacatcc cgcagcaccg gctctatttt cacgagtacg
      301 ccgcgcccga caagatcgcg tacaaggtcc ggcaagtcat cgactggctg aatggtcccg
      361 aagcgaagaa gatgaagaac ccgctgcggg tcgccgcgcg agtgcacttc gacttgttgc
      421 gggcattccc gttccagtcc gacagcggga aggtctcacg cctattcatg aacgtgattc
      481 tgatgcgcgc gggttaccca cccaccatca ttcactccac cgaacgccag aagtactacg
      541 aggcgctgaa gggccagttg ccgatcatca tcacgatgac caacgaggct atcaacaacg
      601 ctctgctgag catcgagaag cgtctcgacg agtacgaagc gcgcggtcga gtcggggtcg
      661 ctccgccgcc cgccgcatcc tgattggacg ggcgtcccag gcatgccgag agctcgtgtc
      721 gagagctctc ggcatcgaga gggcctcgat gaggctagcg gaactctcag aagaagctca
      781 atcgtccgcc gcgattacgt cccagccagt ggcttgcgcg agcttgccta ccgcaacgtt
      841 gtagtcgaac gtagctgaca tctgcccgaa gcggcgcagc gcgtactctt tggccggatc
      901 gacgatgtcg gcgtcttcga acgtcccaac gtcgatgccc tgctgcaccg caatcatcca
      961 tttcttcgcg aagcccacgg ctcgcgtcag ggcatctagt cggcgctcgg cgtcttcggc
     1021 ttcgcgatag gcttgttcga cttccacgcc cactcccccg agagcaaagc gctcggttgc
     1081 ccgttgctcc tcgagctggg cgcgtgcctg ggcataacga gccgactgcg gcaggaaatc
     1141 cagcttgtag cgcacggcca atgaggcgcc gtaggactgc gcgtgcccaa cgtctcggac
     1201 aaacgggttt cgttgatcgg tgacgtacgg cgcgttcacg atcgtcgcgc cgagagccag
     1261 acctatgtcg gggaagaaac ctgcccgttg ttggcgaagc tgcgcttcgc gcgccaacac
     1321 tcctgcgcgc gccatattga cctcggggcg gtaaaggcga gctgccgata agtagcgagc
     1381 gagcggcgct agatgatggt tcacgcgctg caacggacga tcgggcacat cgaaggtcgc
     1441 ggtctcgact acccccgtga aaaaccgcag cccggccatg gcgatctgct cttgcttccg
     1501 ggcttcgctc tggcgaacca ccagatcttc ccgctgaatc tggaggcgcc agagatcgat
     1561 ttcgtccccc tcaccctcct tgaggcgctc cgccagatgc gccgcgtatt tgtccactcg
     1621 ggacaacgct tcgtcgacca gcgtcagcgc atcgcgcgtg agcagcacgc cgtagaatgc
     1681 gcggcggacc tgcagcttca agtcgttctt ctctttgcgg atctcttgct cgcccacttt
     1741 gacttgggca tccgcggcgt cccacagatt ggtgatcttt ccgaaggtcc acagcgggac
     1801 caagcctgtc aggctgccct gccaacccac gctcatcttc gaagtgatcg gcagatcgct
     1861 cgaagggctg tatagcgccg tgccctgaac cacgggcgcc ggtgcaaacg cgaagctgag
     1921 cgtaaactcg ctaaacggag cggtgcgcgc ttgaaacgac tgagcccgct tctgttcgag
     1981 ccgagctcgc gcttcgtgca ccttggggta attgcgttcg gcgagctcta gacaacgacg
     2041 cagtccgacg ccggttttgc gtcgttgcag cacaccgctc tcggcctcgt tgggagcagc
     2101 ggccgaggga gccgcggtcg tcgcgtcctc cgctgaagaa gtggcattac ccgcggcgct
     2161 cgcgggccgc tcttgagcca gcgctgtctg ccaaaccaat gaactcacgg tgctcgcggc
     2221 catccacctg aagagccgcc cagccgatcg gtacgtcatg cgtcgtcgtc gcgcattctg
     2281 actccagagc gcttcgccgc acgcctgaaa ttggatcgat ctagaccggc ctgtcgagcg
     2341 gcctccgaga cgttgcctcc ggcacgttgc aggagtgcgt tgaaatacgc cgtttcgaag
     2401 gcgtgcacgg cgcgatcctt ggcttcccgg taaggcaaat cggccaacgg agccgaaaac
     2461 ccagacggat cgggggtggg ctggatcagc ggctccgcag ccgtggattg cttgcgtgta
     2521 aacggcaggt ctgccggagc gatcgtggtg tcccgacaaa aaaccaccgc gtgttcgatc
     2581 gcgttctcga gctcacgaac gttgcctgac caacgttggt tttgcagcac ggccatggca
     2641 tccggcgaaa tcttgcgcac cgggctattg gatcgcgctg cgtacttctg caggaaatgg
     2701 tacgcgagca gcgggatgtc ctcttttcgc tcccgcaacg gcggcaacga aatggcgacc
     2761 acgttgagcc ggtaatacag gtcttcgcgg aagcggcccg cttgaatgcg gcccttcaag
     2821 tccacgttgg tcgcggcgat cacgcgcaca tcgacgatcc gcgtctcgtt cgatccgacg
     2881 cgcttgactt cgccctcttg gagagcgcgc agcaacttca cctgagcgat cgggggcaaa
     2941 tccccgacct cgtcgaggaa caacgttccg cgatcggcgg cttcgaacaa gccggggcgc
     3001 gtcgcagtgg cacccgtgaa tgcaccgcgg acatgcccga acagctcgct ttccaccaaa
     3061 tccaccggaa tggccgagca attcacgggg atgaaggggt tgttggcgcg cggggaatgc
     3121 tgatgaattg cacgcgccgt gagctctttt ccggtcccgc tctcgcccaa aatgagcacc
     3181 gtcgatgacg tgggcgcgac cccaacggcg agtcgataga cttcctgcat ctcggtcgag
     3241 ttcccgatca gctccccaaa cttctcgagg tgctcgagcc ttcgctccag catcgccgcg
     3301 cgatcgacca agcgacggcg ctcggccgcc ttggtgatgg taaccaccac ctcgtcgttg
     3361 ttgcgaaacg gcttcgtcaa gaagtggtag gcgcccgcgc gcaccgcttt cacggccgtc
     3421 tcgacgtcgg caaaggcggt catgattacc acttcaacgc cgctatgctc cgcgcgcacg
     3481 tggtcgagca gctcgagccc acctactcgc ggcatcatca gatccacgag cgccacatcc
     3541 acgggctcgc gcctcagcac gtccatcgcg accgcgccat cgtccgccgt caccacgccc
     3601 atcccccgac tgagcaacag gcgggccaga cttcgacgca gcatcggttc gtcgtcgacg
     3661 atcaagactc tcggcggcgt atcctcgagc ctaacctccc cgaactcacc agagcgggca
     3721 cgtagcgtga gagtcactcg atccccctgc gttgcgcctc gctttccgag ggaagccgtg
     3781 cgcggggagc ggtagcccct gacgtcgtcg catcaccctg ggtgctaccc gagaccggtg
     3841 tgtagagctg gccttgctgc tggtacacgc aaactcgatt tttgccctcc cgcttggcgt
     3901 tgtgcaacgc cgtatcggca gctttcaata actgatcttt ggtgcgaacg tctcgtgaag
     3961 ggaagagcgc cacgcccacc gacaggctcg ctcgcaccgg ctccggcagg tctccttgga
     4021 tgggtgtttc agagacgtca cgccagatgc gctccgccac cgtgacggat cccgcgaagt
     4081 gcgtactcgg aagcagcacc aggaattcat ctccgccaaa gcgggccacg acgtctactt
     4141 cgcgtacgca gcggcgcaag gcttcggcga cgccgcagat cacgcggtct ccgtgccgtc
     4201 gcccacccgc ctggttgtgg ctctcgagcc gatcgatatc gatgaccaag caggccagag
     4261 gatcgtgata tcgctcggcc cgcttgaatt cctcgttcat gcgcgggtga aggtagcgat
     4321 agttgtacaa gccggtcagc tcatcatgca cgctgagttg ctcgagccgc cgacgtgctt
     4381 cggcgacgtg gtcgtgcagc ttcttgatgc gaagcatggc ttcgacacgg gccgtgagtt
     4441 cgcgttcgtc gaacggcttg caaacgtagt cgtctgcgcc gattttcagg ccttcgacgc
     4501 gactcgcgct atccgtcttc acggtgagga gtaacaccgg caagaagctg tcgtgggtca
     4561 tgcccttgat caggcggcag gcctcgagcc cggagagttt gggcatcacg atgtcgagca
     4621 gcaccacatc cacaccgccg cgggcaatgc gctcgaccgc agcttgacca tctccgaaag
     4681 cctctacgac ataaccgtga gcgcgcaaca cgctgctcaa agcttcgcgg gtcaggcgat
     4741 catcctccgc caccacgacc gttgaacctc gcacggggcg tgcaacttca gataccgaat
     4801 tcgaggcgtc ccgctccgac acgagaaaag gctactcgaa ccttgggtct ttgacgacac
     4861 catgcgaaca ggcggaccgc aatcttgtca caccatggaa ccggaggacg ctttgcccga
     4921 ggtagctatc ttcggattca tctcgggaac agctccaaac ccacgttgac gtcgaagccg
     4981 cggcgccctt cacgacgcga gccaccgaga tgaaacgaca cgcacttaca acggtggcgg
     5041 tacgcgagtt cggcagctga cgcgagcgcg cgccgggtgt ccaagtcgaa gtcggacccg
     5101 acgctcgccg cgaaaccgag gagcaagggc gatcccaagc gcgcacgcag gctggtgcct
     5161 tgcatggaca gactgcgacc ccaaagcgct aaatcactct cgagagtcga gagcagaact
     5221 cgagcgctgt ttcgagcatc caacctcaag ccaagccgtg ggccgtccac ttcgccgagc
     5281 tctgcgcgca ccgaggtctc gagcgctctg gtcttcggat ctcccgcgag caacccgctg
     5341 gcgcgcacgc tgccccacgc cgcgctgagc tccgaagcga gcagcggcgt gagctcggac
     5401 tttccgcgca ctgcaccgcc cgcacccaag ctccactcca gcgcgcgccg ggcccccgcc
     5461 ccggcctgcg aaaaggcgat ctttgcgccg gtgatggcac tcgcaaagaa cccgctgcgt
     5521 aagtcggccg cgaaccaaat cggctcttcg agacgagcca cgagcgtgct cagctccgca
     5581 tagggttcga gccaagtggt gctgctctcc gagcgtcgaa gcagcggtag cccgagccgc
     5641 gagcgcaacg ccgaacgcga cgccgaagcc gcgtccgttt cgttcgcggc ccagctcaag
     5701 cgctggcgca gctcgatatc ccaaagcaga gccgcgatgg gcacggagac cgaagcaccc
     5761 caacctagat tcccgacggc cagagcgcca ttggcggccc aagccgatcg tgtttcggca
     5821 tggaagccga gctgcgcgga ctttccgaga gatgtgccga gtccaagcgt gaaaccaggg
     5881 cccatccatg cccccccttg ggcaccgaag tcgtccaacg cgcggcctcg tctcgcatcc
     5941 gcctccagtg tcaccgcgaa gccgtgctgc cccgcgctac cgacggccag gcgcactcgg
     6001 tccttcggca atgccacccg ctcgagggtt gccggcgcga tctgcgcgcg ggtcccccgc
     6061 agcacctcca cgcgctcggc ccagaccgcg cccgattcgg agcgtgcggc ccactgcgaa
     6121 gagatgtcca acgagttcgc gcgctctaga tagtcccagc cgacacgaga gaaacctgac
     6181 tcggtttgga cctcgatcga gctcccgaag ccaccgcggg agtagcccca agcgcgcagg
     6241 tcgaaccggc tcgggtttcg tccaagggga ccgagcggca ggtgaaaccc agatccaaag
     6301 acgaaccctt cctcaccccg gtaagcgaag ttgggtggca gcaaaccaac ccgatcggcg
     6361 gctcgcaacc acaagaaggg cgaccaaaag agcggcagcc cgagcagcct caaggtgccc
     6421 ccgtacagca agatatcggt cggaggcgcg aagtcggccg acgcgaaacc cagagtaacc
     6481 ggcggctcgg ggcaggggca aaagctaagc ttgccctgcc ctacgacgtg caacccgcgg
     6541 cgggagcgcg agagccggat gtcggagctc gtgatctgga agcgctcggc gcggaccacg
     6601 acgtccccgg aaagacgtaa gctttcgcca tcgccgtcca cctcgacgtg ccgcgcctga
     6661 aacttcacct cgtgctcgcg cgggggggcc ggctttgccg cttgtgcgaa agcccgagag
     6721 ccaacaccag acagcccgca cgccaccaag caagccaaga gcgcccggtg accccgagac
     6781 aacaccgggg ggctcacgtg ccggctcgaa acgccgccat cacccgtcaa aacgcgcgag
     6841 ctggaccaac ctgggccgca gccggcggtg gtcttcgtcc gctggcggcg cgataggccg
     6901 cgtcgagcgt cactccgagc gaaacataga aacgtgccca cgggctgtag aacaagctgc
     6961 gtcgctgccc gtcttctccg agctgcgcgg cgatgttgat atagccgagg cttgccgcca
     7021 agtagtcgtt gaacgtgtac atcaactccg tggcaaagat ggtgcggacg gtgctgcggc
     7081 tggcgtcgtt gagtcgaatg ggcgcgacac agccggtgcc caagttgcag acgtccacgt
     7141 catcggtcag cgcgtaccgg cgatcgtagc ggaagccgaa atcggcggac cagccgagtt
     7201 tttccacgag cgccgtttcg aaacgcagtg agccgagcac cacgtgttga ggtagcgccg
     7261 agcctgacag ctggtcactt ggagccgagc gcccctcggg gtcgacgcgg acacgttgca
     7321 ccgtatcgtt cgtggccacg ttggagcgcg cgaaatgata gctgtagttg atgcgcgcgg
     7381 agaccttgcc cttcggtaaa aagtaggctc cgggaccacg cagtggaact tcttgatcga
     7441 tgcctgtgcc gatacctacc ccaagaattc tcccgcttcc ggcgctggct ttcgaagtag
     7501 gaaccacgag ataaggaaat cgaatcagta cggaggtccg ctgattctcc actcgtcgaa
     7561 gatgctcgaa ccaagccaac cacagctcgg tatcgtacgg cgcccattcc ccacgtcgtg
     7621 tcgtggtgtc actgttggtg aactcgcgcg ccaagcgaaa atcgagacgg aggctgagat
     7681 cacgccgctc gttctcgacc aggtaaaagc gtggccacag gcgcagcgtg atgtcatagg
     7741 tcgggttgtc gctcaagtac tgcgaaccaa cgccgaccgt ttgcgtcgtg atcgactggt
     7801 cgagcaccag cagggagtcg tgaaatcggc tcaccgcttc gcggggaggc gcgggctcac
     7861 tcgcgggaac gactgggcgc cccacgccgt gatccgctgc gaccccggtt ggggtgcgtt
     7921 gcgcctgtgt ttgagcgagc cctggctggg cgaaccctgc tggcgccgcg cctgtgtcgg
     7981 cggcttgcgc ttgcgcggtt tggggcgcgc tcagcgtgaa cgcgcacagg agtccgccga
     8041 accagcgtac gagcatgtcg gtcatgagtt tatccgggcc cgagctttgg cccaagccgc
     8101 aacgcccccc aatctacttg gaaacaggag ctcgcccaag acaacgcggc ttggctccgg
     8161 gaaggctttg cgcgcgctcg tggaaacggc tatgagcgcg accatgccgc cagcccttcc
     8221 ggctacggaa ataacagcgt tacttggccg aggcacccac ttcgaaggca agctcgattt
     8281 cgaaggacgg gtgcgcatcg atgggagctt tcgcggacaa atcaaaaccc gggacgtttt
     8341 gatcgtcggt gacggcgccg aaatagaagc cgagatcgag gcgggtaccg tgatcgtaaa
     8401 gggcggcact ctcagcggaa acgtccacgc gacggagggc atcgagctgt atgtccctgc
     8461 gcgcgtcaac ggaacactgc gggcgcctga gatattcatg gacaagggtg tgcaattctc
     8521 cggaacgtgc accatcgagc cccgtactcc gactgaacca ggttgaacat cgtcgaggcg
     8581 cgtcgtccat tgcggttcat tgcagccctc ctcgcgcgcc gcactgcggc ccaacgtcag
     8641 cgaaacagtg attcgctcgg aacggcgagc gtaacaccct gatacacggc tagtttgcca
     8701 cggccgtact cctcgtgaac cctgacagag agcaagtcgt ctctctttcc acgtcggcgt
     8761 aaaaacactg gacgatgagg cagccttggc accgttggca tgccgtgctc gctgacctgt
     8821 tgccagtttg cgccaccgtc agcgggagtt accagtaccc tggccgttga ctgacgcgaa
     8881 cccggataca gcctgagttt cgtgcacgaa aacgaaactg catcttgcgc tctcgtcgcc
     8941 gcgccacccg gcacgcgtcg tgcaaactcg gcgaggcaga cgaggttaat gactatgaag
     9001 cagtcagggc tttccgaccg acatgcgttc ggccgcatcc ttcgagcggc tgcgttgacg
     9061 gccacactga gcggagcagc ggccgcccaa gcggatgcac gcgagcgacc ggtggagcgc
     9121 tacgatgtgc gcacagctgg gacgagcgcg ggagcagcgc ggacggttgt ccgtgcaggt
     9181 gacggtgttg tccgctccgt ggtgactgac ttcagtcgat atccggattt catcagtcgt
     9241 ttcaagacgg ccaaggtcat cggcaagtcc ggggacaaga ccgacgttta ccttcaggtg
     9301 cccatcctca acggcgccgt aaagatatgg gcggtcgtgc gcttcgatcc cccgaaagag
     9361 gtcaacggtg gccaagtcgt ctcgggcaaa atgctgaagg gcaatgtcaa acgcttggac
     9421 gccgtttggc ggatccagcc gctggatgcc gaatcgacgg agctgaagtt ggaactgacg
     9481 attgtgcccg acctccccgt ccccgaagcc gtcgtgctgc ccgaggtccg ctatgcggcc
     9541 gcgaaagccg tgtcaggtac tcgagacgag gccgaacgcc gggcgacaca ctgacgtttg
     9601 tctcaggttg ggccgttaac ccgacgcgct ccgagctttc ggaacaattg cgctgaccag
     9661 gttcatttcg acacgccgcc aaaggccgga ttcgcacctc gaatccggcc ttttttcgtg
     9721 atttttacgg aggcgagcgc gctgagcgca cctttacccg ccgttgcctg atgcctccca
     9781 ggatgcgggg cagaagcgct attgaaacgt tacggtcgta aagttgaggt gaatgtcgta
     9841 gaagtcaggg tgcgccgtga gctggaaaca gcgcggcgcg ttatccgccg cggggcctaa
     9901 atctgctccg ccgtcccgct tgggtcgtgt caggctgcaa aacccccact tccgcctgac
     9961 aggatgacaa agggcaatat aattgcccgc tcaaggagtg cgccatggtc ctaagatgcg
    10021 cacccacctt tcaaccctaa accccacacc gacaaagaca tgccgcttcc gccccgacga
    10081 ggactttacg acccacgttt cgaacacgac gcctgcggcg ttgggttcgt ggccaatatc
    10141 cggggggagg cctcgcacga catcgttaag aagggggtag atatcctcct caacttggtt
    10201 caccgcggtg cgtgcggctg tgaccctttg acgggtgatg gcgcgggcat cctgctgcag
    10261 ctctcgcact cgttcttcaa gcgagagagt gatcgcgcgg gtatcgattt accggagccg
    10321 ggaatgtacg gcgtcggaaa cgtcttcctg ccccgcgaca agacgcagcg gcgccgttgc
    10381 caagagatcc tggaagacaa gatctcgggg acgggccaaa aattgctcgg ctggcgcaac
    10441 atccacgtga atgaggacgc gctggggcca atggcgcgcg cgagcgtgcc cgtgatccgc
    10501 caagtgttcg tcggcgcgac cacgacgaac caggacgagt tcgagcgtaa gttgttcgtc
    10561 atccgcaagt gggcagagcg cacggtccgg gaaagcaaca tcgcgagccg cggcatgttc
    10621 tacatgccga gcctctcctc gcggaccatc gtgtacaagg gtctgctgct cgcagagcag
    10681 ctcacgggct tctatcgcga actagaagac ccggagatga agagcgcgct ggccatggtg
    10741 caccagcgct tcagcacgaa cacgttcccg acctgggagc tcgcgcaacc attccgcatg
    10801 ctcgcgcaca acggggaaat caacaccgtg cgtggcaact ccgcgtggat gagcgcccgc
    10861 gagcagttga ttgatgcgca cgcggtgttc ggcaacgact tccgccacat tctgcccacg
    10921 atcgcgccag ggggcagcga ctcggcccaa ctcgacaacg tcgcggagct gctgctccac
    10981 gccggacgtt cgctgccgca cgtgatgatg atgctcgtgc cggaggcctg gcaaaacgac
    11041 ccgttgatgc ccgagcacaa gcgcgacttc tacgagtacc actcgtgctt gatcgagccg
    11101 tgggacggcc ctgccgcgct ggcgttcact gacggcaagc gcattggcgg catcttggat
    11161 cgcaacggtt tgcgcccggc gcgttggacc atcacgaaag acggtctcgt ggtgttggcg
    11221 tcggaaacgg gcgtgctcga ctttgcgccc gagaacatcg agcgcaaagg tcgcctcgag
    11281 ccgggccgca tgttcttggt ggacacggag cgcggtcgca tcgtcgaaga cgaagagatc
    11341 aaggacgaga tctgcaagcg ccgcccttac ggcaagtggc tgcgggagaa caagatcgcg
    11401 ctcgacgaga tcgaggattc caccggctcc tcgaaccacg tggagcgcac gtcgctgctg
    11461 gatcgccaga agatcttcgg ctattcgcaa gaagatctga agatgttgat ggcgccgatg
    11521 gcggcccaag gcgaagaggc ggtcggctcg atgggcaccg acacgccgct cgcggtgctg
    11581 agcgacgagc cccaactgct cttcaactat ttcaagcaac acttcgctca ggtcaccaac
    11641 cccgcgatcg atccgatccg cgaggagatg gtgatgagct tgaaggcgta catcggcggc
    11701 gagggcagcg tgctagctga gttgcccgat cacgcgcaga tgctcgagct cgagcatccc
    11761 atcctaacga acgcggatat cgcgaagctg cgggagacac acctgcaaca ggtgcgagtg
    11821 cctccgacgc tgccgatgtt gtacccggtc gcggaaggcg gcgccggttt gaaggaggcg
    11881 ctcgacgagc tgtgtcgcca agcgtcgctg gcggtgaacg atggctatag cttgctgatt
    11941 ctgagcgacc gtggggcgac cgacgcgctc gcgccggtac cgagcctgct cgccacgggc
    12001 gccgttcatc atcacctgat gcgcaacggt acccgcatgc gcatcgggat catcgtcgag
    12061 accggcgagg cccgcgaggt gcatcacttc tgcttgctga ccggctacgg cgcaggcgcc
    12121 atcaatccct acctcgcgtt cgagacgatc gagaacatgg tgcgcgaagg caccttcccc
    12181 gccctaaagg acgccgagct cgcgaagaag aagtacatca aggccatcaa caagggcttg
    12241 ctcaaggtca tgagcaagat gggaatcagc accctgcaga gttatcacgg ggcgcagatt
    12301 ttcgaggcca tcgggctctc gcccgaggtg gtcgatcgct acttcacggg caccaccagc
    12361 cgcatctccg gcatcgatct cgacgtgatt gccgaggaga gccgccggcg ccacgaagtg
    12421 gctttcccga aggccaagca gcaactcccg ctgctgccga tgggcggcca gtaccactac
    12481 cgcgcgcagg ccgagcgtca cctgtggaat cccaccaccg tcgccaagct gcagcatgcc
    12541 gtgcgcatgg aggacgtgaa gagctacaag gagtacgccg cgttcatcaa cgacgcgcgc
    12601 gatgggctca tcacgctgcg tggcatgtgg gacctgaagt ccgatcgcgc gccggttcct
    12661 ctcgaagagg tggaaccggc ggcggagctc gtgaagcgct tcgccactgg ggcgatgagc
    12721 ttcggctcga ttagcgccga ggcccacgag aacctcgcga tcgcgatgaa tcgcatcgga
    12781 gggcgcagta acaccggtga aggcggggag cgcgaggaac gcttccgccc cgactccaac
    12841 ggggacttgc ggcgtagctc gatcaagcaa gtggcgtctg ggcgcttcgg cgtgacgacc
    12901 cattacttgg tgaacgccga tgagctgcag atcaaggtcg ctcaaggcgc caagcccggt
    12961 gagggcgggc agctgccggg ccacaaggtg gacgcgatca ttgcgaagac gcgccactcc
    13021 acgccgggag tgacgttgat ctcgccgccg ccgcaccacg acatctattc gatcgaggat
    13081 ctcgcgcagc tgatcttcga tttgaagatg gtgaatccgc gggcgcgcat cagcgtcaag
    13141 ctggtcgccg aagcgggcgt gggcaccgtt gcggccggcg tggcgaaggc ccacgccgac
    13201 gtgatcttga tctccggctt cgacggtggc accggcgctt ctcccctcac ctcgatcaag
    13261 cacgcgggcg tgccgtggga gatggggatc gcggagacgc accaggtgct ggtcaagaac
    13321 gatctgcgga gtcgcgtgat tctgcagagc gacggtcaga tgcggaccgg tcgggacgtg
    13381 atcatcgcgg cgctgttggg tgccgaggag ttcgggttcg cgacggctcc gctggtggct
    13441 tcgggctgca tcatgatgcg caagtgccac ctcaacacct gcccagtcgg catcgcgacc
    13501 caggatccag tgttgcgggc gaaattccag ggcaagcccg agcacgtcat ccgcttcatg
    13561 ttctacgtgg ccgaagaggc tcgtgagtgg atggcaaagc tcggcttccg cacggtgcgt
    13621 gagctcgtgg gtcacgtgga gtgtctggag ccccgccgcg acgtccagca ttggaaagct
    13681 acacggctgg atttctcgga tattctcgct ccggccgaag ccgccccggg cgtcgcggtt
    13741 tgcaagacaa tcgagcaaga ccacggcctc gacaaggcgc tcgacatcga gctgctgcgc
    13801 ttgtgcggcg cgggactgga gcggggggag cccgttcgag ccgatctacc catccgcaac
    13861 gtgcaccgca ccgtcggtgc gatgctggcg ggcgaggtcg cgcgattgca cggcaaggcg
    13921 gggctcccag aaggcacgct gcgctttggc ttcaatggct cggcaggaca gagcttcggc
    13981 gcgttcttga cgaacggcat cgaattctcg ctcgagggcg acgccaatga ctacgcgggc
    14041 aaggggctcg cgggcggcgt aatcgcggtg cgaccgccgc gcggagcgtc gttccgcgcc
    14101 gacgagagca tcctgatcgg caacaccgcc ctgtacgggg caacgagcgg gcgagccttc
    14161 ttcaatgggc gcgctggcga gcgcttcgcg gtgcgcaata gcggggccac cacggtcgtg
    14221 gagggcgtcg gtgaccacgg ctgcgagtac atgaccggtg gtcgcgtgct cgtactcggg
    14281 cgcaccggcc gcaacttcgc cgcgggcatg agcggcggct atgcctacgt gttggacgag
    14341 gccggcgatt tcgagaaacg ctgcaatctc gggatggtag agctcgaggc gctgagcgcg
    14401 gaggatgcgg ccatcgtcgc ctcgctgctc gaagaacatc gccaacgcac cggcagcccg
    14461 aaggcgatct ccctgctgaa caattgggaa agcgccttgt ctcgattcgt gaaggtggtg
    14521 ccggtggagt accgccgcgt tctcgaggag ctcgcccgac gcgaaaagac ggctccgagc
    14581 agctcggctg tgctacacgg aagcccctaa cttaggcctg actcactaga ccggcaggtc
    14641 cccggcttcg agctgttcag ctccgggcca acgatcggaa aaaggggccc gccggctctc
    14701 gcgggtgggc tctgacgggt cgaatcctgg cggattgagt cgttggagcc tgtcagcgtg
    14761 cgggtgacgg cgatcacgcg accgtctccc gaacgcgacc taagtctcct gaacacagac
    14821 tcgaaacgtc tgtcagcgta cccccaagcg ccccctcacc cgaatgcgcc ctttcgaggg
    14881 caacctcaac ccgaagttgt gccgtgggaa aagttaccgg atttttggag ttcaagcgcg
    14941 ccgaaccctc gaagcgtccc gtcgacgaac gtgtgaagga ttatcgggag ttcgagcttc
    15001 cggtggtcaa cgagacgttg gtcgcgcagg gcgcgcgctg catggactgc ggcgtgccgt
    15061 tttgcaactc gggttgccca ctcggcaatc tgatcccgga ctggaacgat cacgtcttca
    15121 agaacgagcc caagctcgcg atcacgtcgc ttcactccac caacaacttc cctgagttca
    15181 ccggtcgcgt ttgccccgcg ccgtgcgagg ccgcgtgtgt gctcggtatc aacgacgatc
    15241 cggtctcgat caagttgatc gagcgatcga tctccgatcg cgcatttggc gagggcctcg
    15301 tgcagcccga gctgccgcga acccagaccg gcaagaaaat cgctgtgatt ggctcgggcc
    15361 cggccgggct cgcggcggcg cagcagctgg cgcgcgtggg ccacagcgtg acggtgttcg
    15421 agaaggacga ccgaatcggc gggctgctg
//
LOCUS       DASTKU010000851        13202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1507253, whole genome shotgun sequence.
ACCESSION   DASTKU010000851 DASTKU010000000
VERSION     DASTKU010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13202
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507253"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2541)
                     /gene="dnaE"
                     /locus_tag="VFQ61_29950"
     CDS             complement(<1..2541)
                     /gene="dnaE"
                     /locus_tag="VFQ61_29950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET9958768.1"
                     /translation="MAEEFVHLHVHSQYSFLTSAVKLSSLAQQAKSLGMRAVALTDQA
                     NMYGAIRHYSACKAAGIQAILGSELNVVRKNGTGFDHLVLIAENEPGYRNLIRLVSEG
                     HLQPANESAPSVTMDQIAERNRGLIALTGCLGGYVAQRVLEYGPDEADVVLGELKERF
                     EPGHLFVELQDHGFPEQTVVNEILVAAAQRLGLPVVATNDVHFTKREDGEAQIYLECV
                     RQGRTYEEAEPLHHKSFEMYLKSAEEMAQSFSKYPEAIKNTLAVAEMCSGLKLQLGTP
                     MLPRFPLPEGYDTDTYFRHVSQQGLERRFNEFELTGKNVDRAVYQARLERELDVIVGM
                     KYPGYFLIVWDFIREAKSRGIPVGPGRGSGAGSLVAYALGITEIDPLPYNLLFERFLN
                     PERVSMPDFDVDFCMARRDEVIRYVAEKYGVDSVGQIATFQNLKAKSVIKDVARAMGF
                     PAQDAQRIASMVPEKGQGKTYTIDEAIEVEPKLKTLVDSDQRVSELMRHSKKLEGLTR
                     HAGMHAAGVVISEGPLADHVPCFKSDATIVTQYDKDDVEAAGLVKFDFLGLKTLTVID
                     IAQRLVNARPDRKGNPIDLTKIPLTDRETYQLITSGETTGVFQLESSGMQQLLRQLKP
                     DCFEDIVAVVALYRPGPLGTGMIDDFVGGKHGKKAIRKLHPLVDDVLAPTYGVPVYQE
                     QVMQIAQFLAGYTLGGADLLRRAMGKKKAEEMAKQQKTFVEGAAKNGVPEEKSAAIFK
                     EIEGFASYGFNKSHSAAYAVVTYQTAYLKAHYPTEFFAAALTADKDKIEKVVRTIAEA
                     RAWGVSVLPPDINLSETDFTVVYARPEGGRPVKPTGKLRDRLCPQIRFG"
     gene            2813..3493
                     /locus_tag="VFQ61_29955"
     CDS             2813..3493
                     /locus_tag="VFQ61_29955"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9958769.1"
                     /translation="MTAKASIFALLFALAVPAVVVACTASASFQAGGSDAPPPPPPPP
                     PPPVGEATATPSATATTTAAPTATATTTATTTAAPTTTTPKPSVSGSSVTLPGTIEFE
                     SGKATFKAGTNSEQSLAQLKQFLDENARITKLRIEGHTDNVGGADANLKLSGERALAI
                     KRWLVEHGVVKERLIAAGFGQTKPVADNATEEGRAKNRRTEFRIAELSGKPYLGNDPT
                     GGGKVFDL"
     gene            3547..4008
                     /locus_tag="VFQ61_29960"
     CDS             3547..4008
                     /locus_tag="VFQ61_29960"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9958770.1"
                     /translation="MAAKLGIAAATLSGCDRPAADLRVWQAADHDHTDQPNAAQVEVK
                     PGATASGPLAEHGIDDVTLATWKENCMRCHGQFGAGDGPQGPMNQTRNLTDPAWQASM
                     TDQQIAQSIHTGKGRMPAFNLPEHTVSSLVRLIRMMKMEARSDETQGADQR"
     gene            3992..4435
                     /locus_tag="VFQ61_29965"
     CDS             3992..4435
                     /locus_tag="VFQ61_29965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958771.1"
                     /translation="MPTNVENRGAPADGRARFRRTGGLVLLLVGVLLSGQLLKGSWPR
                     ERTLIFRIPQAACQTATQLTAHFTEVGEREPFHGLTMALTPPSPRNLRQRITIPDGDY
                     IVTLELTYGSITGPIAPEKPETSRARRVSLSSDETLVEFDAEGSE"
     gene            4432..5811
                     /locus_tag="VFQ61_29970"
     CDS             4432..5811
                     /locus_tag="VFQ61_29970"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9958772.1"
                     /translation="MTQTSQPPEVSTRAQGSVPPMPDELVLTVLSGSARGKKLKVGSQ
                     LTIGKATDNDLVLPDDTVSRHHCVLERRTDGIFVRDLGSTNHTRVGRTTIREASIEPG
                     ATITVGNVELVLRSEPNRAQILPSESNQFGEVIGTSLSMRSIFGLLERIAPTDACVLI
                     EGETGTGKDVLARSIHQQSARSEHEFLVVDCGAISYNLIESELFGHERGAYTGAVSSR
                     QGAFELAGKGTVFLDEVGELPLDVQPKLLRVLESGEYRRVGGNKNMHAEARIVAATKR
                     NLREEVERGKFREDLYFRLSVVPVTVPPLRQRREDIPALVERFLDQARKRVPAAANIV
                     LSRETMAALSAHDWPGNVRELRNVLDRAIYVAAAGGENEVRLVDVPVSPTPIGVAPGA
                     NAFPSFEAGKSYREVRADFEADFERRYVSWLLDRHAGNISAAAREAKMDRKHLYDLAR
                     KHGLRGERG"
     gene            complement(6003..7250)
                     /locus_tag="VFQ61_29975"
     CDS             complement(6003..7250)
                     /locus_tag="VFQ61_29975"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HET9958773.1"
                     /translation="MARPGSIPRPSLSAVGAALLLSVPSAAQVADVQLRGNFFYEPSA
                     TSHVTVLNPTLDASAQPVEGVRVRAGYEADIVSGASEAVKAGRLTSVDVVSSATSFHD
                     TRQVASGGVTLARESTELSATYAYGTESDYRSHSIAVSAKTSFLQRNTELSLGYARGF
                     DQVCTSAFATAIAPSARIALDSSKGCFTKAENRASRDVDLDTLRAGWTQAWSPIWSTQ
                     LVATFALQHGFLANPYRSVVIAPAGDVALENHPENRARAALTLRSRWYLRGPKLAVGA
                     SIHLYRDTWDIDAQSYELEAERYLFSALRVAVRARYYHQGAALFFSDDYTGGEPADGP
                     RGQYWSGDRELSRLSNYGLGARIAYARRAEPNTRILGILKGVSASLGVDALKTHLSGF
                     TWAGVEPDDTLALLASLALGSEF"
     gene            complement(7295..7573)
                     /locus_tag="VFQ61_29980"
     CDS             complement(7295..7573)
                     /locus_tag="VFQ61_29980"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HET9958774.1"
                     /translation="MKILQVATALQTLDRAVRRFGVGTLFWAGALIILLSAAGCATVR
                     PEQRAVLADPIMQFDSEPGEQAARAHVLENREGALASGSIKGGGCGCN"
     gene            complement(7582..8418)
                     /locus_tag="VFQ61_29985"
     CDS             complement(7582..8418)
                     /locus_tag="VFQ61_29985"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET9958775.1"
                     /translation="MGHSVFDSVIGALGLRLLAIRAWANRGFARSALVAGATSAALLT
                     SAHARAADPDVFARVVVSETELRSGPGISYRAIQRVRRGEVFLVKTRETKGFWLQVEL
                     PDGRTAYVLGDTVATQTVDSDAPDAPHKPGLFAPPALQSSRAGFTLLAGLYDGSGYAE
                     IRPALVIAPAIAFEPYVGVALGPDSRRLIYGGAGTLNLAPDWAIAPFFEVGLGGVLEQ
                     PKDEFVRPDRKWFHARAGGGLLVSLRLRLLLRLEVSNVVLFNEDDYQNRQAYLAGLGT
                     YF"
     gene            complement(8391..8921)
                     /locus_tag="VFQ61_29990"
     CDS             complement(8391..8921)
                     /locus_tag="VFQ61_29990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958776.1"
                     /translation="MAPALPSAIARLTQRLFGSGILLFISFACDGCGDQGFLPGTVER
                     GPDFNVAEVVFDAGYYYCKVEPMLVERRCGPGDSAQGDPAGGCHANVTSYRLTEYMPL
                     VADSCTDGLHPDVSPSAVAQQNYQASQVKMRRDPDAAPLLMRPTGRAQHPRTLFDTSS
                     AEADLIRTWATQYSTQ"
     gene            9539..9706
                     /locus_tag="VFQ61_29995"
     CDS             9539..9706
                     /locus_tag="VFQ61_29995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958777.1"
                     /translation="MKLNPWYLAPLPIALLAAHEAFGAQLAVLGVTLGIFFGTLSLRR
                     PQAGDATTTHH"
     gene            9754..10797
                     /locus_tag="VFQ61_30000"
     CDS             9754..10797
                     /locus_tag="VFQ61_30000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04552 family protein"
                     /protein_id="HET9958778.1"
                     /translation="MPHGPRLKRLEEFSLHDLESVRLILAGASVVDWHRLHLADPAQS
                     RRFLENHELRLENPGDRKFAEHIQKEAVSYLRRHFSFAIPKPVERASIEELLLVASGR
                     GHRQVCACTILKAVQIINHMAGRELLFRLPVSDRDLFHLVEEKVYRVVGTMLSDGFPI
                     TEFVGGRKNLDSTYTKLLSKPEATAAALYDKLRFRIVTRTREDILPVLLYLTNQLFPF
                     NYVVPKQSTNTIFHLGTLMHSHPHLAGMAKELQGPEDDRLLPNDNRFSAPTYRVIQFV
                     TDVPVRVPPHLMELAPPGSENLGPVVYVLCEFQVLDAESEAVNETGEASHDRYKMRQR
                     AAVFRRLRRGARP"
     gene            10889..11398
                     /locus_tag="VFQ61_30005"
     CDS             10889..11398
                     /locus_tag="VFQ61_30005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HET9958779.1"
                     /translation="MRLYSARLPKLAHEIVQGLVKDHDIETDSPREVELDVEAVLAQY
                     MRDEQAVSDRAKDVIAQRGLPQSELNRIKKLVADERKIKIGEDSIDYILDQLVEMLMH
                     STNVEEIFVEDYVLRRKMRDPLRKVASEETDLQTEVRAQLKHVQEGSSVWEVEYRRMM
                     EDIRRRKGL"
     gene            11408..12277
                     /locus_tag="VFQ61_30010"
     CDS             11408..12277
                     /locus_tag="VFQ61_30010"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HET9958780.1"
                     /translation="MRSMTGFGIGEATLGEGRVTLELRGLNHRFLDVRVRLPPEWAEH
                     GFFVEQLARERLARGRFDIGVRLAGASLPSARFSADKARAVYAALLELRDAVAPGSEV
                     PLSSVLMVPALVSSPPEEDLSPRDALQGALDMAVRHFDQMRLREGEALRRDLFERLAQ
                     CRALVAQIRERSGLLPELYRNRLRERADQLLSGCSLRVEPARLELEIALFADKVDICE
                     ELARLGSHFDQFEELAVGSQNSGRRLDFLLQEIGREANTVGGKCQDAAIAHLVVELKA
                     QIERMREQVQNVE"
     gene            12267..12923
                     /gene="gmk"
                     /locus_tag="VFQ61_30015"
     CDS             12267..12923
                     /gene="gmk"
                     /locus_tag="VFQ61_30015"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET9958781.1"
                     /translation="MSNDEPLQLIISSPSGAGKSTLTARLIREVGDLRFSVSHTTRRP
                     RETELNGREYHFVDRTEFEALIEAGAFLEWAEVHGNLYGTSRSEIQRSRAARGIIFDI
                     DHQGARQIKSCSPDAVAVFILPPSMRVLEERLRGRRSEDDATVRRRFDAAREEIAHYG
                     LFDYVLVNDDLEEATRKLTSIFLAEECRRARASRYAERLLAEGRGFGGTLPPGRPPRA
                     "
     gene            13014..>13202
                     /locus_tag="VFQ61_30020"
     CDS             13014..>13202
                     /locus_tag="VFQ61_30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958782.1"
                     /translation="MISSPQRNASVLIVGSMAFDDLELPTENAKDVVGGAATYAAYAA
                     SLFAAPRLVAVVGSDFPES"
BASE COUNT         2521 a         4205 c         4074 g         2402 t
ORIGIN      
        1 cccgaagcga atttgcggac acaagcgatc acgcagctta cccgtcggct tcaccggtcg
       61 gcctccctcg gggcgcgcgt aaacgacggt aaagtcggtt tcgctcaggt tgatgtccgg
      121 cgggagcaca ctgacgcccc aggctcgcgc ctcggcgatc gttcgcacca ccttctcgat
      181 cttgtccttg tccgcggtca gggccgcggc gaagaactcc gtgggatagt gcgccttcag
      241 ataggccgtt tgatacgtga cgacggcgta cgcggcggag tggctcttgt tgaagccgta
      301 cgacgcaaag ccttcgatct ccttgaagat ggccgcggac ttctcttcgg gtaccccgtt
      361 cttggctgca ccttccacga aggtcttctg ctgcttggcc atctcctcgg ccttcttctt
      421 acccatcgcg cggcgaagca gatcagcgcc gcccagcgta tagccggcca ggaactgcgc
      481 gatctgcatg acctgctcct ggtagaccgg cacgccgtaa gtgggggcga gcacgtcatc
      541 caccaggggg tgtagcttgc ggattgcctt cttgccgtgc ttgccgccca cgaagtcgtc
      601 gatcatgccc gtaccgagcg gcccgggacg gtagagggct accacggcga cgatgtcctc
      661 gaagcagtct ggcttgagtt gacgcaggag ctgctgcatc ccgctagact cgagctggaa
      721 aacgcccgtg gtttcaccgc tcgtgatgag ttggtaggtc tcgcgatcgg tgagcgggat
      781 cttggttaaa tcgatcggat tgcctttgcg atccgggcgc gcgttcacca ggcgctgcgc
      841 gatgtcgatg accgtcagcg tcttgagacc gaggaagtcg aacttgacga gaccggccgc
      901 ttcgacgtca tccttgtcgt actgcgtgac gatggtcgcg tcgctcttga agcaaggcac
      961 gtgatcggcg agcgggccct cgctgatcac gacgcccgcg gcgtgcatgc ccgcatgccg
     1021 tgtcagccct tcgagcttct tgctgtgacg catcagctcg gagacccgtt gatcggaatc
     1081 gaccaacgtc ttgagcttgg gctcgacctc gatcgcctcg tcaatggtgt aggtctttcc
     1141 ttgccccttc tccggcacca tgctcgcgat gcgttgcgcg tcctgcgcgg gaaaacccat
     1201 ggcgcgcgcg acgtccttaa tcacgctctt ggccttcaga ttttggaagg tcgcgatttg
     1261 cccgacgctg tccactccgt acttctccgc gacgtagcgg atgacttcgt cgcggcgagc
     1321 catacagaag tcgacgtcga aatcgggcat gctgacgcgc tccgggttga ggaagcgctc
     1381 gaacaacaag ttgtaaggta gcggatcgat ctcggtaata ccgagcgcgt aagccaccaa
     1441 cgaccccgcg ccggaaccac gccccggccc caccggaatc ccgcgtgact tcgcctcccg
     1501 gatgaagtcc cacacgatca agaagtaccc cggatacttc atccccacga tcacatcgag
     1561 ctcgcgctcc agccgcgctt gatagaccgc acggtcgacg ttcttgcccg tgagctcgaa
     1621 ctcgttgaag cggcgttcga gcccctgctg ggagacgtgc cggaagtaag tgtcagtgtc
     1681 gtagccttcc ggcagcggaa agcgcgggag catcggcgtg ccgagctgca gtttcagccc
     1741 cgagcacatc tcggccacgg ccaaggtgtt cttgatcgcc tccggatact tgctgaagga
     1801 ctgcgccatc tcctcggcgc tcttcagata catctcgaag ctcttgtgat gcagcggttc
     1861 ggcctcttcg taggtccgcc cctgcctcac gcactccaaa tagatctgtg cttccccgtc
     1921 ttcacgcttg gtgaagtgaa cgtcattggt ggccaccacc ggcaagccca gccgctgtgc
     1981 cgcagccacc aagatttcgt tgaccaccgt ctgctcggga aagccatgat cttgtagctc
     2041 gacgaacaag tgaccgggtt cgaatcgctc ctttagctcg ccgagtacga cgtcggcttc
     2101 gtccggcccg tactcgagca cgcgctgcgc gacgtagccg cccaagcaac cggtgagcgc
     2161 aatcagaccg cggttccgct ccgcgatttg atccatggtg acgctcgggg cggactcgtt
     2221 ggcaggctgg agatgacctt ccgaaacgag ccggatcagg ttgcgatagc ccggttcgtt
     2281 ctcggcgatg agcacgagat ggtcgaagcc cgtgccattc ttgcgcacca cgttgagctc
     2341 ggatcccagg atggcttgaa tgccggcggc cttacacgcg gagtagtggc ggatggcgcc
     2401 atacatattg gcttgatccg tcagcgccac cgcccgcatt ccgagtgact tcgcttgctg
     2461 cgcgagcgag ctcagcttca ccgccgaagt caggaaggaa tattgagagt gaacgtgcag
     2521 atgaacgaac tcttcggcca tggtggtcgc acgcgaggct tagcagcact aaaccgaagc
     2581 agcttggggc cgcgctcgga agcctcgaaa aagctccgcg ccgccggccc gggccggccc
     2641 cgcgagacac cgattgaaca cgtttggccc ccagcccgtc catgactcgc cacgtcttgc
     2701 gccctgatcc tgtcgggccc ttgcgcgcgg ctggtgactt ggattaccgc tcaatcgctg
     2761 cacccaggcg gccccggccg accgaccgcc gtagctggag gaacttcttc gcatgaccgc
     2821 aaaagcttcc atattcgcac tgttgttcgc gcttgccgtg cccgctgtcg tggtcgcgtg
     2881 caccgcatcg gcttcgtttc aggcgggagg ctcggacgcg ccgcccccgc cccctccgcc
     2941 gccccctcct cctgttggag aagcaaccgc taccccgagc gccactgcca cgacgacagc
     3001 cgcgcccacg gccaccgcca ccaccacggc gaccacgacc gccgcaccga cgaccacgac
     3061 tccaaagccc tctgtttcgg gcagctctgt cacgttgccg ggaaccatcg agttcgagag
     3121 cggcaaggcg accttcaaag ccggcaccaa tagcgaacaa tcgctcgctc agctcaagca
     3181 attcttggac gagaacgcgc gcatcaccaa gctgcgcatc gagggtcaca cggacaatgt
     3241 gggcggcgct gatgccaacc tgaagctatc cggtgagcga gcgctcgcga tcaagcgttg
     3301 gctcgtcgag cacggagtgg tcaaggaacg cttgattgcg gcaggtttcg gtcaaaccaa
     3361 gcccgtagcc gacaacgcga cggaagaggg ccgagctaag aatcggcgca ccgagttccg
     3421 aattgcggag ttgagtggca agccctacct cggcaacgat ccgaccggtg gcggcaaagt
     3481 gttcgacctg tgatgctgcg tctcttcaca caccggtctt ttgagtgccc gccgttggcg
     3541 atattcatgg ctgccaagct gggtatcgcc gcagcgactc tgagcggctg tgatcggccg
     3601 gctgcagact tgcgcgtgtg gcaagccgcc gatcacgatc acacggacca gcccaacgcg
     3661 gcccaagtcg aggtgaagcc cggcgcaacc gcgagcggtc ctctcgccga gcacggcatc
     3721 gacgacgtca ctttggccac ttggaaagag aactgcatgc gttgccacgg tcaattcggg
     3781 gctggtgatg ggccacaagg gcctatgaat cagacgcgca acttgaccga tcccgcctgg
     3841 caagcttcga tgaccgatca gcagattgcg caatccatcc acaccggcaa aggacggatg
     3901 cccgcattca acttgccgga acacacggtg agttcactcg tgcgtctgat tcgcatgatg
     3961 aagatggaag cccgctcgga cgagacgcag ggtgccgacc aacgttgaaa atcgaggcgc
     4021 gcctgccgac gggcgggcgc gcttccggcg aaccggaggg ctcgtactcc ttctcgtcgg
     4081 cgtcctgctc tccggtcagc tgctcaaagg ttcgtggccg cgagagcgaa ctttgatctt
     4141 tcgcattccg caagcggcct gccaaaccgc aactcagctc accgcacatt tcacggaagt
     4201 gggcgaaaga gagccatttc atggcctcac gatggcactc acaccgccat ctccccgcaa
     4261 tctccgacaa cggatcacaa ttccggatgg tgactacata gtcacactag agctcacgta
     4321 cggctccatt acggggccta ttgcccccga aaaaccggag acaagccgcg cgcggcgagt
     4381 tagtctctcc tccgacgaga cgctcgtcga gttcgacgcg gaaggatcgg agtgacacag
     4441 acctctcaac cccccgaagt ttcgacccgc gcacaaggca gtgtgccgcc catgcccgat
     4501 gagctcgtgc tcaccgtgct gagcggaagc gcgcgcggca agaagttgaa agttggctct
     4561 caactaacga ttggcaaggc cacggacaat gacctggtcc tgccagacga taccgtctca
     4621 cgacaccact gtgtgctcga gagacgcacg gacggcatct tcgtccgtga tctcggctcg
     4681 accaaccaca ctcgagtcgg gcgaaccacc attcgtgagg catccatcga gcccggcgcc
     4741 accatcaccg tgggaaacgt cgagctcgta ttgcggtcgg aacccaaccg agcgcagatt
     4801 ttgcccagcg aatcgaacca gttcggcgaa gtgatcggga ccagcctgtc gatgcgcagc
     4861 atcttcgggt tgctcgaacg catcgccccc accgatgcct gcgtgctgat cgaaggtgaa
     4921 acgggcacgg gcaaggacgt gctcgcccgg tccattcacc agcagagcgc gcgcagcgaa
     4981 cacgagttct tggtcgtgga ttgtggcgca attagctaca acctgatcga gagcgagctg
     5041 ttcgggcacg agcgtggcgc atataccggg gcggtttcgt ctcgacaggg cgccttcgag
     5101 ctggcgggca agggcaccgt gtttctggat gaggtcggag agctgcctct cgacgttcag
     5161 ccgaaactct tgcgcgtgct cgaatctggc gagtaccgcc gggtgggcgg caacaagaac
     5221 atgcacgccg aggcacgcat cgtggcggcg accaagcgca acctgcgcga ggaggtggaa
     5281 cgcggtaagt tccgtgaaga cctgtatttc cgtttgtccg tggtgccggt cacggtgcct
     5341 ccgttgcgtc agcgtcggga agacatccct gctctggtgg agcgcttctt ggatcaggcg
     5401 cggaagcggg tcccagcggc tgcgaatatc gtcctgagtc gtgagacgat ggctgcgctc
     5461 agcgcccatg attggccggg aaacgtgcgc gagctgcgca acgtcttgga tcgcgccatc
     5521 tacgtggcgg cggccggggg cgagaacgaa gtgcgtctag tcgacgtgcc cgtctccccg
     5581 acgccgattg gcgtagcgcc cggcgcgaac gcattcccgt cgttcgaggc gggcaagagc
     5641 taccgcgaag tgcgcgccga cttcgaggct gatttcgagc ggcgctatgt gtcttggctt
     5701 ctggatcgtc acgcgggcaa catcagcgcc gccgctcgag aagcgaagat ggatcgcaag
     5761 catctgtacg atctcgcccg caagcacggc ctgcgcggcg aacgcggctg aggccgctgc
     5821 ttaagcctta cgagaggggt cttagaggct ctttgccgga ccgtgagcgg cacgcgatct
     5881 cactcctctg cgcctgacct cgggcgcgga ggagtgagct tgagttgctc gcggcggctg
     5941 gctcgaagcc gggacttggg gatcggaccg ggcgcgcggc tcggtccaat gcctgcggcg
     6001 cttcagaatt cgctccccag agccaacgaa gcgagtagag cgagcgtgtc atcgggctcg
     6061 acgccggccc aggtgaagcc ggacaagtgc gtcttcaaag cgtcaacccc cagggaggcg
     6121 gacacgccct tgagaatacc gagaatgcgc gtgttgggct cggcgcgacg agcataagcg
     6181 atgcgcgctc ccaagccata gttggacagt cgggagagct cgcgatcgcc cgaccaatat
     6241 tggccacgtg gtccgtcagc aggttccccg ccggtgtagt cgtcgctgaa gaacagggct
     6301 gcaccttgat ggtagtaacg cgcgcgaacc gcaacgcgca gcgcgctaaa aaggtaacgt
     6361 tccgcctcca gctcgtagct ctgtgcgtcg atgtcccagg tatctcgata caaatgaatg
     6421 ctcgcgccga cggcgagttt tggtccgcgc agataccaac gtgagcggag ggtcagcgcc
     6481 gctcgagcgc ggttctccgg gtggttctcc aaagccacgt cgcccgccgg tgcgatcacc
     6541 accgaccgat acggattggc caagaaacca tgttgcaaag cgaacgtggc cacgagctga
     6601 gtcgaccaaa tcggtgacca ggcctgggtc cagcccgcac ggagggtgtc gagatctacg
     6661 tcacgactcg cgcggttctc cgccttggtg aagcacccct tcgagctatc cagggcaatc
     6721 cgcgcgctcg gagcgatcgc ggtcgcgaag gcgctcgtgc agacctgatc gaagccccgc
     6781 gcgtagccca ggctgagctc ggtatttcgc tgcagaaaag aggtcttggc gctgaccgcg
     6841 atcgagtgcg agcgatagtc gctctcggtt ccgtaagcgt acgtcgcgga cagctcggtg
     6901 ctctcgcgcg ccagggtgac tccgccgctc gcgacctgcc gtgtgtcgtg gaacgaagtt
     6961 gcggaagaga cgacgtcgac gctcgtgagc cgcccggctt tgaccgcttc gctggccccg
     7021 gacacgatat ccgcttcgta acccgcacga actcgcaccc cctcgacagg ctgagcactc
     7081 gcgtcgagcg tcgggttgag caccgtcacg tgactggtcg cgctgggttc gtaaaagaag
     7141 ttgccgcgca gctgcacgtc cgcaacttga gcggcgctgg gcacgctgag caagagcgcc
     7201 gcgccgaccg ccgacaagct tggtcgtgga atggagccgg gccgcgccat ggatcgaacc
     7261 cgagctccgc ccgacggcac gaacgaagac ctgctcaatt gcagccgcag cctccgcctt
     7321 tgattgaacc actcgccagc gctccctcgc gattctcgag cacgtgggcc cgggcggcct
     7381 gctcgcccgg ttcgctatcg aattgcatga tcggatccgc gagcacggca cgctgttcgg
     7441 ggcgcaccgt cgcgcagccc gccgcactca gcaggatgat tagcgctccc gcccagaaga
     7501 gcgtcccgac gccaaaccgg cgcaccgctc tatcgagcgt ctggagtgca gtggccacct
     7561 ggagaatctt catgatgcgc ctcaaaaata ggttcccagc ccggccaaat acgcttggcg
     7621 gttctggtaa tcgtcttcat tgaacaacac cacgttcgaa acctccaggc gtagcagcaa
     7681 acgcagccgc agagaaacca gcaggccccc gcccgcgcgc gcgtggaacc atttgcgatc
     7741 ggggcgcacg aactcatcct tcggttgttc caacacgccg ccgaggccca cctcgaaaaa
     7801 cggagcaatc gcccaatccg gggccaagtt cagcgtgcct gcgccaccgt agatcaagcg
     7861 gcggctatcc gggccgagcg cgacgccaac atagggctcg aacgcgatcg cgggagctat
     7921 caccagcgcg gggcggatct ctgcgtaacc gcttccgtcg tacagccccg cgagcagcgt
     7981 gaagccggcg cggctcgact gcagagctgg gggcgcgaac aggcccggtt tgtgcggggc
     8041 atcgggcgcg tcgctatcca cggtttgcgt ggccacggtg tcgcccagca cgtacgccgt
     8101 gcgaccatcc ggcaattcga cctgcaacca gaagcctttg gtctcgcgcg tcttgaccag
     8161 gaacacctcg ccgcgcctca cgcgttgaat cgcacgatac gaaatgccgg gcccgctgcg
     8221 cagttcggtt tccgagacga ccacccgcgc aaagacgtct ggatccgcgg cccgcgcgtg
     8281 ggcgctggtg agcaaggccg cgctcgtggc ccccgcaacc agcgcagagc gagcgaatcc
     8341 gcggttggcc cacgcgcgaa tcgcgagcaa gcggaggccc agcgctccga ttactgagtc
     8401 gaatactgag tggcccatgt gcgaatcaga tcggcttcgg cgctgctcgt atcgaacagc
     8461 gttcgcggat gttgcgctcg acccgtggga cgcatcaaca gtggggcagc gtccgggtcg
     8521 cgccgcatct ttacctgaga tgcctgataa ttctgttgcg cgaccgcgga cgggctcacg
     8581 tccgggtgca gcccgtccgt gcagctgtcc gccacgagcg gcatgtactc cgtcaggcgg
     8641 tagctcgtga cgttcgcgtg gcagccgcca gcaggatcgc cctgcgcgct atcccccggg
     8701 ccgcaacgtc gctcgaccag catcggctcg accttgcaat agtagtagcc cgcgtcgaac
     8761 acgacctccg ccacgttgaa gtcggggccc cgctcgacgg tccctggcaa gaacccctga
     8821 tctccacagc cgtcacacgc gaagctgatg aaaagcagaa tgcccgatcc gaagagccgt
     8881 tgagtgagcc gcgcaatcgc actcggtaga gctggcgcca cgtcgtacaa cgctacaccg
     8941 ggattgggtg aagacgcgag gcatgcgatc gcgcaggtcg tttggcttca gagtcgggct
     9001 aaaaaaaagg ctcttcagtg atctgaattt cgaccgtcac gagctttttc gcgaaaacca
     9061 gcactcggcc tgccgggcaa tctgttcatc gagcccaatt tgacgcccaa tcgcctccat
     9121 gtacccacat aaataacctc cgaaacacgg tcgcacttga atcggccgac ttacatcggg
     9181 taactctttg ccccgctgcg gccgtctgtg gcacagtcac ttacacgctc ctaccttatc
     9241 cacttgagtt ctgctttcgg cttagtcacc gctcgccttt gagggggatt caccggtcga
     9301 atcgtcagtg tcacttggct agcttccctg taacgcgggt ttcctctaaa agagctgttc
     9361 gagccaactc ccttcgattt agaacgcgag ttacccggca gaacccgacc actccggccg
     9421 agcccgaaag gcggccgagc ccgaaacgga tacagctacc ccgagccact ggcaccgacc
     9481 tcgacgccca ccgagttcgc aagtcatcag cgaatcaacg cagagtttgg aaaccagcat
     9541 gaagctcaac ccgtggtacc tcgcaccgct tccgattgct ctgttggccg cacacgaagc
     9601 gttcggcgca caactcgccg tactcggcgt gactctcggg attttcttcg gcacactctc
     9661 tcttcggcgt ccccaagccg gtgacgcgac cacgacgcac cactgaacga gctcgactac
     9721 gacccgccgc gttcatccga acgcattccg accttgccgc acggaccacg gctcaagcgt
     9781 ctggaagagt tttctctcca tgacctcgaa tccgtacgcc tgatcctcgc aggcgcttcg
     9841 gtcgtggatt ggcatcgtct gcacttggcg gatcccgccc aatcgcggcg ctttttggag
     9901 aatcacgagc tacggctcga gaacccagga gatcggaagt ttgcggagca cattcagaag
     9961 gaagccgtca gttacctccg gcggcacttc tcgtttgcca ttccgaagcc ggtagagcgc
    10021 gcgagcatcg aagagttgct gctggttgcc tcaggccgcg ggcaccgcca agtctgcgcc
    10081 tgcacgatct tgaaagcggt ccagatcatc aatcacatgg ctggtcggga gttgttgttc
    10141 cgactcccgg tgagcgatcg tgatctgttt cacctggtgg aggagaaggt ctaccgggtg
    10201 gtgggaacga tgttgagtga cggcttccca atcacggagt tcgtgggggg ccgaaagaac
    10261 ctggactcga catatacgaa gttgctctcc aagccggagg ctacggccgc cgcgctctac
    10321 gacaagcttc gctttcgcat cgttacgcgc acgcgagagg acattctccc ggtgctgctg
    10381 tacctgacga atcagttgtt cccgttcaat tacgtagtcc cgaagcagag cacgaacacg
    10441 attttccact tggggacgct gatgcattcc caccctcacc tggccggaat ggcgaaagag
    10501 ctgcagggtc cggaagacga tcggctgctg cccaacgaca atcggttctc ggcgccgact
    10561 taccgcgtaa ttcagttcgt gaccgacgtc ccggtgcgag ttccccctca tctaatggag
    10621 ctggcacctc cggggtcgga aaatctgggc ccggtcgtct acgttttgtg cgagtttcaa
    10681 gttctggacg ccgagagcga agccgtgaac gagacagggg aagcctcgca cgaccgttac
    10741 aagatgcggc agcgagcggc agtgttccgg cgtctgcgac gcggcgcacg gccatagtcg
    10801 atcggccact gggcctgaag gccaatcgga tcgggcttcg cgaaaaactc ggagcgcatt
    10861 cgcgcacgaa atggggtagt gaagcgccat gcgcctatac agcgcgaggc tccccaagct
    10921 agcgcacgaa atagtccagg gtttggtcaa ggaccacgac atcgagaccg actcaccgcg
    10981 tgaggtggag ctcgacgtgg aagccgtgct cgctcaatac atgcgtgacg agcaggcggt
    11041 cagtgaccgc gcgaaagacg tgatcgcgca gcgcgggctc ccccagagcg aactgaatcg
    11101 gatcaagaag ctcgtcgcgg acgagcggaa gatcaagatt ggcgaagact cgatcgacta
    11161 catcctcgac cagctggtcg agatgttgat gcactccacg aacgtggaag agatcttcgt
    11221 cgaggactac gtgctgcgcc gcaagatgcg ggatccgctg cgtaaggtcg ccagcgaaga
    11281 gaccgatctt caaaccgagg tgcgcgctca actcaagcac gtacaagaag gcagctcggt
    11341 ttgggaggtg gagtaccgcc ggatgatgga ggacattcgc cggcgaaagg gcctgtagtc
    11401 gaagaccatg cgcagcatga ccggcttcgg gattggcgaa gcaaccctcg gtgagggtcg
    11461 cgtaacgctg gagctgcgtg ggctcaatca tcgatttttg gacgttcggg tgcggctgcc
    11521 gccggagtgg gcagagcatg ggttcttcgt cgagcagctg gcgcgagagc ggctagcgcg
    11581 ggggcgtttc gacatcgggg tgaggctggc gggagcctct ctcccgagcg cgcgcttctc
    11641 cgccgacaag gcgcgtgcgg tgtacgccgc attgttggaa ctgcgggatg ccgtggcgcc
    11701 ggggtcagag gtccctctca gctcggtgct gatggtcccc gcgttggtta gctctcctcc
    11761 cgaggaggat ctgagcccgc gcgacgcgct gcagggcgcg ctcgacatgg ccgttcgcca
    11821 tttcgatcag atgcggctac gcgaaggcga agcgctgcgt cgggatctgt tcgagcgtct
    11881 tgcccaatgc cgcgccttgg tcgcacagat tcgtgagcgc tccggactgt tgcccgagtt
    11941 gtaccggaat cgtctccgcg agcgggcgga tcagctgctt tcaggctgca gtttgcgggt
    12001 agagccagct cgcttggagc tcgagatcgc gctgttcgcc gacaaagtag acatctgcga
    12061 agagttggcg cgcttgggga gccacttcga tcaattcgag gagctcgcgg tcggctcgca
    12121 aaactcgggg cggcgactcg actttctctt gcaagaaatc gggcgagaag ccaatacggt
    12181 cggcgggaaa tgccaggacg ctgccatcgc gcacctggtc gtggaactca aagctcagat
    12241 cgaacgcatg cgagaacagg ttcagaatgt cgaatgacga gccgcttcag ctcatcattt
    12301 cctcaccttc tggtgcgggt aaatccacgc tcacggcgcg gctcatccga gaagtcggcg
    12361 atcttcggtt cagcgtctct catacgacgc gtcgcccgcg cgagacggag ctcaacggcc
    12421 gtgagtacca cttcgtggat cgcaccgagt tcgaagcgct gatcgaagcg ggcgcatttc
    12481 tggaatgggc cgaagtgcac ggcaacctgt acggcaccag ccgatcggag atccagcgct
    12541 cgcgcgccgc acgcggcatc atcttcgaca tcgatcacca gggcgcccgc cagatcaaga
    12601 gctgctctcc ggacgcggtc gcggtgttca ttctgccgcc gagcatgcgc gtgctcgagg
    12661 agcgactgcg cgggcgacgc agcgaagacg atgcaaccgt gcggcgtcgc ttcgacgccg
    12721 cacgcgagga gatcgcgcac tacggtcttt tcgactacgt gctggtgaac gacgatctgg
    12781 aggaggcgac ccgcaagctc acgtcaatct tcctggccga agagtgccgt cgagcacggg
    12841 cgtctcgtta cgccgagcgg cttttggcag agggccgggg cttcggtggc accctgcccc
    12901 cgggacggcc gccgcgcgct tgaactgcgc gcggaccctc gacgaacgcg cgccgaatcc
    12961 acaactccaa gttcgattcc gaaaacgcag ctgactttca gagtgattcg acaatgatct
    13021 cctccccgca gcgcaatgca tcggtcctga tcgtaggctc catggcgttc gacgacctgg
    13081 agctgcctac agaaaacgcc aaagacgtgg tgggaggcgc ggccacctat gccgcgtacg
    13141 cagcgagtct gttcgcagcc ccccgcctgg ttgcggtcgt gggcagcgat tttcccgaaa
    13201 gc
//
LOCUS       DASTKU010000852         4028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_612033, whole genome shotgun sequence.
ACCESSION   DASTKU010000852 DASTKU010000000
VERSION     DASTKU010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612033"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VFQ61_30025"
     CDS             complement(<1..684)
                     /locus_tag="VFQ61_30025"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9958783.1"
                     /translation="MKDVLLLDGHCHLASTRYIPESFLSGAAANMAARAKAYGNKTAN
                     QGMLTDVLVRQHQDHTGDVLVREMDAAGIARTVLLYPDFGLALDGCPEPEVAIEEHLA
                     VCERHAGRFELFAGVDPRRERAFERFERFVGAPHLKGLKLYPACGYSASDAALDPFYE
                     LCGARGMPVLFHSGPTTPALFYEFTAPFDVDRAAKRFPRVNFIIGHGGVHDTEQASLL
                     CAHRPNVFLD"
     gene            complement(681..1388)
                     /locus_tag="VFQ61_30030"
     CDS             complement(681..1388)
                     /locus_tag="VFQ61_30030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958784.1"
                     /translation="MSERSEGICRVDGDINITVEPPEEKVARVARQLKEIGRSATLAI
                     TLRIGRLIHQEIFAGKLQGALGSRARKTFRQLANHEETPFSMTALWRATALYEMSLRL
                     PQAFELENLGISHYRSVLGLPEELQLELLTLAASEAWTKSELERQAQIARLHNRAVPR
                     KSRVRLARSLDAIHERLLHWDCHLGAQEFGGDATRVLEGIQKIRSCCSRIEARLSTVA
                     AATVEHGESRGGSQGGV"
     gene            2294..3088
                     /locus_tag="VFQ61_30035"
     CDS             2294..3088
                     /locus_tag="VFQ61_30035"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET9958785.1"
                     /translation="MQTMESRVQGEVSTMQEFWKLARGAMSTAQHMIDRNVQSLQHAS
                     MEQVRSVLLQYRWFTHYYSGDLAILIYKLPPSSLRSFLAECLLEEQGMGKPSETHPAL
                     YDAFLASLGVSSDVFETSLDPDNAAILEEMRSLLLAGDYMYAVGLRGMGAECLCQVYL
                     ESVHHYLLKNPEIIARRSDINWTFWDIHASEADQMHGEKSREFIERLATTPEKIRSLA
                     AGYRDAEQMFLRFWKNAYAALEKSKTRGLAKAPIIPVDGWRYDAAR"
     gene            3075..4010
                     /locus_tag="VFQ61_30040"
     CDS             3075..4010
                     /locus_tag="VFQ61_30040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9958786.1"
                     /translation="MPRADADCIGAADASLMGRLRRVVAARPEQTAISYADGALTYAA
                     FWDQVELAAKALIASGVLVGDRVASWSPNRPECIVIQCAALLCRASVVHLDPELDGAE
                     AMGVLEQLPCRHVFVRANHDGKQYPAILRALRRERGFACEVTVHGRPERFERVLPGGW
                     LDFLDSGRAVTSAALAGRESANGSEDASRVVAFCAGREGAPVKLSAAALSGEARPFEA
                     EAAMSWAVAAFSEPTVLATSCFGALLAGGELSLTDAFTDAEDFLRLVRTHRFSQVFAP
                     RTRIEAVSQAPNSAELLGNCRLIESGSKAAWAART"
BASE COUNT          721 a         1244 c         1302 g          761 t
ORIGIN      
        1 gtccaggaac acgttcggcc ggtgcgcgca gagaaggctc gcttgctcgg tgtcgtgaac
       61 gccgccgtgc ccgatgatga agttgacgcg cgggaaccgc ttggcggcgc ggtccacatc
      121 gaaaggcgcc gtgaattcgt agaacagcgc gggcgtcgtg ggcccgctgt gaaaaagcac
      181 tggcatgccc cgcgcgccgc agagctcgta aaatggatcg agcgccgcat cactcgctga
      241 atagccacac gccgggtaga gcttcagccc cttgagatgg ggcgccccga cgaagcgctc
      301 gaagcgctcg aatgcacgct cgcgacgcgg gtcgaccccc gcgaaaagct cgaagcgccc
      361 cgcgtggcgc tcgcataccg cgaggtgttc ctcgatggcg acctcgggct ccgggcagcc
      421 gtcgagggcg agcccgaagt cggggtagag caacaccgtg cgagcgatgc cggccgcatc
      481 catctcgcgc accagcacgt cacccgtgtg atcctggtgc tgccgcacga gaacgtcggt
      541 gagcatgccc tgatttgcgg tcttgttgcc gtaagccttc gcgcgggccg ccatgttggc
      601 cgctgcccca gagaggaacg actccgggat gtagcgagtc gaagccaaat ggcagtgccc
      661 gtcgagcaac aggacgtctt tcatacgccg ccctgtgagc cgccccttga ctccccgtgt
      721 tcgaccgtcg cggccgcgac cgtcgagaga cgcgcctcga tgcgactaca gcacgatctg
      781 atcttctgga tcccttcgag tacacgagtc gcgtcgccgc cgaactcctg cgcgcccaga
      841 tggcagtccc aatgcagcag ccgctcgtga atcgcgtcca agctgcgcgc caaacgaacc
      901 cggcttttgc gcgggacggc acgattgtgc agccgcgcga tctgagcctg ccgctccagt
      961 tcggatttgg tccaagcctc gctcgccgcg agtgtgagta gctcgagctg caactcctcg
     1021 ggtaaaccca acaccgagcg gtagtgcgag attcccagat tctctagctc aaacgcttga
     1081 ggcaatcgca ggctcatctc gtagagggcg gtggcccgcc acagcgccgt catgctgaaa
     1141 ggcgtttctt cgtgattcgc caactggcga aacgtcttgc gagcgcgact gccgagcgca
     1201 ccctgcagtt tgcccgcgaa tatctcctga tgaatcaacc tgccaatgcg cagcgtgatc
     1261 gccaacgtgg cagaccgccc tatctccttc agctggcgag caacccgcgc gaccttctcc
     1321 tccggcggct cgacggtgat attgatatcg ccgtctaccc gacagatacc ttcgctcctt
     1381 tcggacacct ccgcctccat ttacgctccg tcctccagct aaacccaccg gttcgcgagt
     1441 tgaccgtctt cgttcagtcg gtcatcaggc agtccgcctc acccgcgaga atagatatca
     1501 ctcccctttc actacgtcca ctccaatcga ctggtttcgc ggttcgtgga tcatgacgag
     1561 ccaaaccaaa cttgttctgg gcgtcatagc agcgagttgg acgagcacgt caagtagcac
     1621 gcgttggcgc agggggatgg cgaagttcaa caccccccgg gccgcctctg aaaagttctg
     1681 ctagccgaag ggttgagaca cgatgttttg ctgcgtctcc ggcgtttccc tggggaaaca
     1741 acggtcgggc cgctacgtcg tcgtatgaag cgcgcgctgc gggaatcaac ccggaagcta
     1801 gcgcgttgga cgcggccgcg cgcgttcgtc ccgcaaacgg caacgcgccc catcacgact
     1861 gcataggtgt tttattcatt cgcggccgtg cgtccgaagg agttcgcgcc ctcgcggtcg
     1921 gagttggtac gaacgtgatt cgtcctggtg ctcgcgtcga cggcgcgcgc ggggtcgcgt
     1981 gcagatatcg agagaagaga tgctccgagt tcgatgtggc ggatattgcc gccctctgtc
     2041 gtcgtgtcgg tttgcgggct tcgcagaggt gcgcccgccg tgcagagctc tcgcgtgtga
     2101 gcgggagtaa aagaactcat atgttcgacg atcgatacac ccatgtgaat tgatatcatg
     2161 tctgacgaat tcgcttttca attggagtgc gccagggtac atgtggggaa ctggaagggt
     2221 gcaatgctcc ccgcgagccc gtggttgggg gcgcgcatcc ctttgtttgg gcaacttgca
     2281 cggaagaggg catatgcaaa cgatggaatc gcgagttcag ggtgaggtgt cgacgatgca
     2341 agagttctgg aaactcgcgc gaggggctat gtctactgcg caacacatga tcgatcggaa
     2401 cgtgcagagc ttgcagcacg cttcgatgga acaagtgcgg tcggtgctcc tgcagtatcg
     2461 ctggttcact cattactact cgggggatct cgcaatcctc atctacaagc tcccgccgag
     2521 cagtctgcgg tcgtttttgg ccgagtgtct gctggaggaa caagggatgg ggaaaccgtc
     2581 cgaaacgcac cccgcgctct acgacgcctt ccttgcgtcg ttgggcgtga gtagcgacgt
     2641 gttcgaaacg agcctcgatc cggacaatgc ggcaatcctc gaagagatga ggagtctact
     2701 gctcgcaggg gactatatgt acgcagtcgg gctgcgcggg atgggggccg agtgcctgtg
     2761 ccaagtgtac ttggaatcgg ttcaccacta cctcctcaag aacccggaga tcatcgcgcg
     2821 gcgttccgac atcaactgga cgttctggga cattcacgcg agcgaagcgg atcagatgca
     2881 cggcgagaag tcgcgcgagt tcatcgagcg cttggccacg accccggaga agattcgcag
     2941 cctcgcggcc ggctaccgcg acgcggagca gatgtttctg cggttctgga agaacgctta
     3001 tgccgctttg gagaagtcga agactcgcgg gctggcgaag gctcccatca tcccggtcga
     3061 cggttggaga tacgatgccg cgcgctgatg cagactgtat cggggccgcc gacgcgtcgc
     3121 tgatgggtcg gctgcggcga gtcgttgcgg cccgcccgga gcagacggcc atctcgtacg
     3181 cggacggcgc gctcacgtac gccgcgtttt gggaccaggt ggagctcgca gccaaggccc
     3241 tgatcgcgag cggtgtgctc gtcggggacc gcgtcgcttc gtggtcgccg aatcgacctg
     3301 aatgcatcgt aattcaatgc gcggcgctgc tatgccgcgc aagcgtcgtt cacctcgacc
     3361 ccgagctgga cggagccgag gccatgggcg tgctcgagca gctgccatgc cgtcatgtgt
     3421 tcgtccgagc caaccacgac ggcaagcagt atccggcgat tttgcgggca ttgcggagag
     3481 agcgcggctt tgcgtgcgag gtcaccgtgc acgggcgccc cgagcgattc gaacgcgtcc
     3541 tgccgggagg ttggttggac ttcttggact ccggtcgcgc cgtcacgagc gcggcgctcg
     3601 ctggacgcga gagcgcgaac ggatccgagg acgcttctcg agtcgtggcg ttctgcgcag
     3661 ggagagaggg cgctccggtc aagctgagcg cggctgcgct gagcggcgag gcgcggccgt
     3721 tcgaggccga ggcggccatg agctgggcgg tcgcggcatt ctcggagccg accgtgctcg
     3781 cgacttcctg ctttggcgcg ctgctcgcgg gcggagagct ctctctaacc gacgcgttca
     3841 ccgacgcgga agacttcttg cgcttggtgc gcacccaccg cttcagtcag gtgttcgcgc
     3901 cgcggacgcg tatcgaggcg gtcagtcagg ctccaaactc ggccgagctc ttgggcaact
     3961 gtaggctcat cgaatccggc agcaaggctg cctgggcggc tcgtacatga tccgcgagcc
     4021 ggtgctcc
//
LOCUS       DASTKU010000853         3328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1729677, whole genome shotgun sequence.
ACCESSION   DASTKU010000853 DASTKU010000000
VERSION     DASTKU010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729677"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VFQ61_30045"
     CDS             complement(<1..1181)
                     /locus_tag="VFQ61_30045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HET9958787.1"
                     /translation="MSSARRSFAIYAITRHGVKIAQRLLPALPGADLLISERLRDLAP
                     DATCIFPLPMGPLLAETFKRYDAHVFVISVGAVVRLIAPLLENKKVDPAVICVDDASR
                     YAICVLSGHVGRGNAFTERVASALDAQAIVTTASDSIGTLTVDILGRELGWNLDDPDR
                     NVTRACAAVVNAERVLFVQESGEPEFWPLNKTLPPGVHYTTSLEGVEPSAWDMLLIAS
                     HRDLARTHPRHFRAAVVYRPKSLVLGIGCDRDTPLELIERGIQHVFAEHGLCTRCIQA
                     LASIDKKADEPALLRLAAARGWPFQTYTAGELARLGSAEGVRSPSDTVQRFVGSPGVC
                     EPAALLRAGAKELLVPKQKYTEPGAGRSMTLAVAEVPFMKRVAALNSGTDDPRTVQPQ
                     NS"
     gene            complement(1178..2023)
                     /gene="cobI"
                     /locus_tag="VFQ61_30050"
     CDS             complement(1178..2023)
                     /gene="cobI"
                     /locus_tag="VFQ61_30050"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HET9958788.1"
                     /translation="MSATSALDAKALGKLSGVGVGPGAPDLITLRALRVIQSADVIAL
                     PRSSDYGASVAWSIARESLALRPEQERLYLTFPMTKHPDQVRQHVFGAVDQIEGHLRA
                     GRSVAFLTEGDPSVFSTFGYVRTEAKRRLPTLEIEVVPGVTSITSVAMVGGVPLADGH
                     ERVAIVPATYGVDDLVTLLQSFDTVVLMKLGAELPRILEALERTGLTEHAFYVSKATM
                     PEQRIERDVRELRDEHGGCFSMLIIKRTDRSGLLMGHCSSAERPSEEDSLAHALLPTS
                     SVEGI"
     gene            complement(2020..>3328)
                     /gene="cbiE"
                     /locus_tag="VFQ61_30055"
     CDS             complement(2020..>3328)
                     /gene="cbiE"
                     /locus_tag="VFQ61_30055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HET9958789.1"
                     /translation="IGDDGCGSLSSRAAGALARAQILVGGERQLAFFPDFAGPRIPLR
                     GDLQAALAQVADLAREQTVCVLSSGDPLFFGIGARIVSAVGSEHVEFIPAPSSVQWAF
                     ARTGISWEDALVLSVHGRSAEGFVARVRSAAKVAVLTDADNNPAALAARLIEFGEFGF
                     EAWVCERLAGPAERVRRFTLEELANTRDLDPLNVLLLSRTDPDWRAPCSVPGLPEEAF
                     AKRIPKQGLITKREVRVLSLSALQLRPDSVLWDVGAGSGSVAIESALLAPKGRVYAIE
                     VDPECVAMCRENARAHGADNVRVIAGLAPEACHGLERPDCVFVGGSKGSLTSIIALAL
                     DVLRPGGRLVVNAITLENIGEAYAALRERQYEPEVTLVQIARGVPLARYLRYESLNPI
                     HIFAVRKGDNVSRAGASEPGTREAEQSPAPAPLPNASEAGGPT"
BASE COUNT          634 a         1111 c         1017 g          566 t
ORIGIN      
        1 gaattttggg gctggaccgt gcgagggtcg tccgtaccag agttcagggc ggccacccgc
       61 ttcatgaagg gtacttccgc aaccgccaag gtcatgctgc ggcccgcccc gggctccgtg
      121 tacttttgct tgggtacgag caactccttg gctcccgcgc ggagcagcgc cgctggttcg
      181 cacacgccgg gggagcccac gaaacgctgc accgtgtccg acgggctgcg caccccctcg
      241 gcggagccga gccgcgcgag ttcacccgcg gtataggttt gaaatggcca accgcgggca
      301 gccgcgagcc gcagcagcgc gggctcgtcc gcctttttat cgatgctcgc tagggcctga
      361 atgcatcgag tgcacagccc gtgttctgca aacacgtgct gaatccctct ttcgatcagc
      421 tcgaggggcg tgtcccgatc gcagccgatt ccgagcacga ggctctttgg acggtagacc
      481 accgccgcac ggaagtgacg cggatgcgtg cgggccaaat cgcggtggga cgcaatcagc
      541 agcatgtccc aggcactcgg ctcgaccccc tcgagtgagg tggtgtaatg aacgccgggg
      601 ggcaatgtct tgttcaaagg ccagaactcg ggttcgccgc tctcctgtac gaacagcacg
      661 cgctcggcgt tcaccaccgc cgcgcaggct cgtgtcacgt tccgatccgg atcgtcgagg
      721 ttccacccga gctctcgtcc gagaatatcg acggtcaggg taccaatcga gtcggatgcc
      781 gtcgtaacga tggcttgggc gtcgagggcg ctcgcgaccc gctccgtgaa cgcattgccc
      841 cgccccacgt ggcccgacag aacgcaaatc gcgtatcgag acgcgtcatc cacgcatatc
      901 accgctggat ccaccttctt gttctcgagc agcggcgcaa tcaagcgcac taccgctccg
      961 acgctgatca cgaacacgtg cgcgtcgtac cttttgaagg tttcggccaa cagcggcccc
     1021 atgggcagcg ggaagatgca ggtcgcatcg ggggctaggt cgcgcagacg ttcggagatc
     1081 aagaggtcag cgcccggcaa cgcgggcaac agcctttggg cgatcttcac gccgtgtcgc
     1141 gtaatggcat agatggcaaa gctgcggcgt gcggaactca tataccctcc acgctcgaag
     1201 tgggtagcaa tgcgtgcgcc aaggaatcct cttccgacgg cctctccgct gatgaacagt
     1261 gccccatcaa cagcccactg cgatcggtac gcttgatgat gagcatcgag aaacagccgc
     1321 cgtgttcgtc acgtagctca cgcacgtcgc gctcgatacg ttgctcgggc atggtcgctt
     1381 tggagacgta aaacgcatgt tctgtgaggc ccgtgcgctc tagcgcctcc aggatccgcg
     1441 gaagctccgc ccccagtttc atcaacacga ccgtgtcgaa gctttgtagt agggtcacga
     1501 ggtcatcgac tccataagtc gccggaacaa tcgccacacg ctcgtgccca tctgccagcg
     1561 gcacgccacc gaccatggcc accgacgtaa ttgacgtgac gccggggacg acctcgatct
     1621 cgagtgtagg caggcgacgc ttcgcttccg ttctcacata cccgaaggtg ctgaagaccg
     1681 acggatcacc ctcggtaaga aaggccacgg agcggcccgc ccgcaggtga ccttcaatct
     1741 ggtccaccgc accgaacacg tgctgacgca cttggtccgg gtgcttggtc atcgggaaag
     1801 tcaggtagag acgctcttgt tcgggacgca gggcgaggct ctctctcgca atcgaccagg
     1861 ccacggaggc cccgtaatcc gagctacgag gcagcgcgat cacatctgct gattgaatga
     1921 cgcgcagggc gcgcagcgtg atcagatccg gggcgcccgg tcccacgcca acgccagaaa
     1981 gctttccgag cgctttggcg tcgagagccg aggtggcact cacgtaggac ctcctgcttc
     2041 cgacgcattg gggagcggcg ccggcgcggg cgattgctcc gcttcacggg ttccgggctc
     2101 gctcgccccc gcgcggctga cgttatctcc ttttcgaacc gcgaaaatat gaatgggatt
     2161 gagcgactcg taacgaagat atcgcgccaa gggcaccccc cgtgcgatct ggaccaacgt
     2221 cacctcgggt tcatactgcc gctcgcgcag ggctgcgtaa gcctccccga tattctcgag
     2281 ggtaatcgca ttcacgacca aacgccctcc ggggcggagc acgtcgagcg ccaatgcgat
     2341 gatgctggtg agactgcctt tgctgccccc gacgaacacg caatctggcc gctcgagccc
     2401 gtggcaggct tccggcgcaa gccccgcaat gactcggacg ttgtccgcgc catgagcgcg
     2461 ggcgttctca cgacacatcg cgacacactc tgggtcgacc tcgatcgcgt aaacacgccc
     2521 cttgggagcc agcaacgcgg actcgatagc aaccgagccc gagccggcac cgacatccca
     2581 caataccgaa tctggtcgca gctggagtgc ggacaaagaa agaacgcgga cttcgcgctt
     2641 ggtaattagg ccctgcttcg ggatgcgttt tgcgaatgcc tcctcgggta gtcccggcac
     2701 cgagcagggc gcacgccagt ccgggtccgt tcggctgagc agcagcacat tcaagggatc
     2761 caaatcgcga gtgttggcca gctcttccag cgtgaagcgt cgcacgcgct cggcgggccc
     2821 tgccaaacgc tcgcataccc acgcctcgaa gccaaactcc ccaaactcga tcagacgcgc
     2881 ggcgagcgcc gccgggttgt tatcggcatc cgtgaggacc gccacctttg cggcgctccg
     2941 cacgcgtgcc acgaagccct ccgcgctgcg accgtggacc gagagcacga gcgcatcttc
     3001 ccaggaaatc ccggtgcgag cgaaagccca ctgcaccgag gacggggccg ggatgaactc
     3061 gacatgttcg cttccgaccg cgctcacgat ccgagcgccg attccaaaga acagaggatc
     3121 gccggagctg agcacgcaca ccgtctgttc acgcgcaagg tctgcgactt gcgccagggc
     3181 cgcctgaagg tcaccccgca ggggaattcg cggtccggca aagtctggga agaacgccag
     3241 ctgtcgttcg ccgccgacca gaatctgcgc tcgcgccagg gcgcccgccg cacggctcga
     3301 taggctcccg cagccgtcgt ccccgatg
//
LOCUS       DASTKU010000854         4257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_166971, whole genome shotgun sequence.
ACCESSION   DASTKU010000854 DASTKU010000000
VERSION     DASTKU010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166971"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2044)
                     /gene="thrS"
                     /locus_tag="VFQ61_30060"
     CDS             complement(<1..2044)
                     /gene="thrS"
                     /locus_tag="VFQ61_30060"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET9958790.1"
                     /translation="MAISVVLPDGKALALEEGATAMDAAKAISAKLAEVVVVARVDGK
                     VQDLRVPLKDGEKLELLKVDTPEGRDTINHSAEHVLAMAVLNLFPGAKVTMGPRTHEE
                     EFYYDFDVGGRSFSPEDLEKIQAEMQRIIDQKVRFERRSYSKTDVLGVFEELGQNNEY
                     KHEILGWIPDDEVSIYQSGQFVDLCRGPHLPHAGFIKGLKITAASGAYWRADASKQSL
                     QRVRGVAFPKKEELDKYLHRLEEAKKRDHRKLGRELELFLVSERYDDHSYTEQQGIEL
                     YVGVEADAPLDRQLVEYTLAQAGEAFPGRSIRQTVNMAVAPKAGPGETEAPKPKLDVR
                     VRGAVAAPARKAFETALQARSDVEANLLVEPYFSEELGPGLVMWLPKGGRLRTIIEDQ
                     WRKMHLASGYDVVFSPHIAKTDLWKVSGHWDFYREGMFSPMSVDGHDYVCKPMNCPFH
                     CLMVKSRNRSYREFPMRLAELGTVYRYELAGVMHGLMRVRGFTQDDAHLFCRADQVEQ
                     EIERVLRFILRMLRTFGFEDFEVNLSTRPEKYVGSLEDWAKAEATLESVVQRFGLKYS
                     VDAGGGAFYGPKIDIKLVDALERKWQCSTLQYDFNNPARFNLEFVNAKGEREQPIMLH
                     RALLGSIERFIGILVEHYAGALPAWLSPEQVRVVTVSDKHNAHAEQLTETLNAQGIR"
     gene            2220..2876
                     /locus_tag="VFQ61_30065"
     CDS             2220..2876
                     /locus_tag="VFQ61_30065"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9958791.1"
                     /translation="MKLYHHPLSSNARRAFMTALLLESPVEAVVVDLTKGAQRQPDYM
                     KLNPNGKVPTLVDGNTVLWESLAIMIYLCEKARSAQPLSEQLYPSDLAGRTEVNKWLF
                     WTASHWSAAAAQLNFENMLKGRFGLGEPNSYAVGRAELLFAEFARTLDATLGKQAYVA
                     GERLTLADIAIAPTLMYTDSAKLPVEGLSNLQRWFASIRELPAWKATEPNIPGFGGKS
                     "
     gene            2930..3628
                     /locus_tag="VFQ61_30070"
     CDS             2930..3628
                     /locus_tag="VFQ61_30070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958792.1"
                     /translation="MVQSVAEAQDLVAFVAELCERIQGQIERLSQDPLRAEEQSWLSS
                     ARSRLQAELHHVQQGFARAALLPEFETERKAKLLGLEQAWVDSLEGLLAGLTRRVGAG
                     NPLVEVLFPHQKFEKLRRSSQRMQSYMADFERRRNSTYVTRMCADPEYPFLNGLLAEV
                     ARAKANWQQELAATPPPTEEQEALRAELLTQATQLSNVLRQARALAEAALTFEPEHWA
                     ALAAEYKSRRRIAR"
     gene            3721..4119
                     /locus_tag="VFQ61_30075"
     CDS             3721..4119
                     /locus_tag="VFQ61_30075"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HET9958793.1"
                     /translation="MAVTPSYLSYVLDQFSGAGEVVSRRMFGGVGLYRDGVFFGAVDD
                     DAVYMRVDEATRPEYERRGMPMLRPLKSKPDLVMQAYFQLPPEILDDREVLHEWLERA
                     VRAAVAAAKPKRPPSARTRKPKAASARKRT"
BASE COUNT          767 a         1462 c         1269 g          759 t
ORIGIN      
        1 cccggatccc ctgggcattc agggtctctg tcaactgctc ggcgtgtgca ttgtgtttgt
       61 cggagacggt cacgacgcgc acttgttccg gcgagagcca ggccgggagc gcccccgcat
      121 agtgctccac cagaatcccg atgaagcgct cgatcgagcc gagcagcgcg cggtgcaaca
      181 tgatgggctg ctcgcgttcg cccttggcgt tcacgaactc caagttgaag cgagccgggt
      241 tgttgaagtc gtattgcagc gtggaacact gccacttccg ctccagcgca tccaccaact
      301 tgatatcgat cttgggcccg taaaaggcgc cgccgcccgc gtccaccgag tacttgagcc
      361 cgaagcgctg caccacgctc tcgagcgtgg cttcggcctt ggcccagtcc tccaagctgc
      421 ccacgtactt ttccgggcgc gtggacaagt tgacctcgaa gtcctcgaac ccgaacgtgc
      481 gcagcatgcg caggatgaag cgcaaaaccc gctcgatctc ctgctccacc tgatccgcgc
      541 gacagaacaa atgcgcgtca tcctgggtga acccgcgcac gcgcatcaag ccgtgcatca
      601 cgcccgcgag ctcgtatcga taaaccgtcc cgagctcggc caggcgcatc gggaactccc
      661 ggtagctgcg gttccggctc ttcaccatca agcagtggaa cgggcagttc atcggcttgc
      721 agacgtaatc gtgtccgtcg accgacatgg gcgagaacat gccctcgcgg tagaagtccc
      781 agtgccccga gaccttccac aagtccgtct tggcgatgtg cggcgagaac accacgtcgt
      841 agccggaggc caagtgcatc ttgcgccact ggtcctcgat gatggttcgc agccgaccgc
      901 ccttaggcag ccacatcacc aggcccggtc cgagctcctc actgaagtac ggctcgacga
      961 gcaggttggc ctcaacgtcg ctgcgggctt gaagcgccgt ctcgaaggcc ttacgcgccg
     1021 gcgccgccac cgcgccgcgc acccgcacat ccagcttcgg cttgggcgcc tccgtctcgc
     1081 ccgggcccgc cttcggcgcg accgccatgt tgacggtctg ccggatcgaa cgccccggga
     1141 acgcttcccc cgcttgggcc agcgtatatt ccacgagctg ccggtcgagc ggcgcgtcgg
     1201 cttcgacccc gacgtacagc tcgatgccct gctgctccgt gtacgagtgg tcatcgtagc
     1261 gctcggacac caagaacaac tcgagctcac gacccagctt gcgatggtcg cgcttcttgg
     1321 cctcttccag ccgatgcagg tacttatcga gctcttcctt cttcgggaag gcgaccccgc
     1381 gcacgcgctg cagtgattgc ttgctggcgt ccgcgcgcca atacgcgccg gatgccgcgg
     1441 tgatcttcag acctttgatg aagcccgcgt gcggcaagtg cgggccgcgg cacaagtcga
     1501 cgaactgccc gctctggtag atggacacct cgtcgtccgg aatccagccc aggatctcgt
     1561 gcttgtattc gttgttctgg ccaagctcct cgaacacacc gagcacatcg gtcttcgagt
     1621 acgagcgccg ctcgaagcgc actttttggt cgatgatccg ctgcatctcc gcctggatct
     1681 tctcgagatc ctcgggcgag aaactgcgcc cacccacgtc gaagtcgtaa tagaactcct
     1741 cctcgtgcgt gcgcggaccc atggtgacct tggcacccgg aaacaggttc agcacggcca
     1801 tggcgagcac gtgctccgcc gagtgattga tggtgtcgcg cccttccgga gtgtcgactt
     1861 tcaacagctc cagtttttcg ccgtctttca gcggaacacg cagatcctga accttgccgt
     1921 cgacacgcgc gaccaccacc acttccgcga gcttcgcgga aatggccttg gcggcgtcca
     1981 tcgccgtcgc gccctcttcg agcgcgaggg ccttgccgtc cgggagaact accgagattg
     2041 ccatgatttt ttccttctcc cccggcggta cgagggccag ggggagcgcg actacaagca
     2101 ctcggcgcct actctgtcaa ccacacgcct cggtgagcag cgcagctttg gccggcgggc
     2161 acgcgcggct ggcacgaaaa ccgcgcgccc ggcgcaaaaa gccgcgatat gctgcgccga
     2221 tgaagctcta ccaccaccca ctctcatcca acgcccgccg cgcgttcatg acggcgttgc
     2281 tgctcgagtc acccgtcgaa gccgtagtcg tggacctgac caaaggcgcc cagcgtcagc
     2341 ccgattacat gaagctgaat cccaacggca aggtgccgac gctggtggac ggcaacaccg
     2401 tgctctggga gtcgctggca atcatgatct acctctgcga gaaggcgcgc agcgcgcagc
     2461 ccctcagcga gcagttgtac ccttcggacc tcgcgggtcg cacggaggtc aacaagtggc
     2521 tgttttggac cgccagtcat tggtctgcgg ccgcggccca gctcaatttc gagaatatgc
     2581 tgaaaggcag gtttggcctc ggtgagccca attcttacgc cgtcgggcgc gccgagttgt
     2641 tgttcgcaga gttcgcgcga accctcgatg cgaccctggg caaacaagcc tacgtcgcgg
     2701 gtgagcgcct cacgctggcc gatatcgcga tcgcgcccac gctcatgtac accgatagtg
     2761 ccaagctccc cgtcgagggc ctctcgaacc tgcagcgctg gttcgcctcg atccgcgagt
     2821 tgccggcctg gaaggccacg gagcccaaca tccctggatt cggcggcaag agctgagtga
     2881 ctgcgcggcg agccagaatg agaagctgtg acgttgcgag atcgggctga tggttcagag
     2941 cgtcgcagaa gcacaagact tggtcgcatt cgtcgctgag ctttgcgagc gaatacaggg
     3001 ccagatcgag cgcctctcgc aagatccgct tcgtgccgag gaacagagct ggctcagctc
     3061 cgcgcgtagt cgattgcaag ccgagcttca tcacgttcag cagggcttcg cacgagcggc
     3121 cctgctgcct gagttcgaga ccgagcgcaa agccaagctc ctcggtctcg aacaagcctg
     3181 ggtggatagc ctcgagggct tgctcgcggg cctcacgcgc cgagtgggtg ccggcaatcc
     3241 gctggtggaa gtgctgtttc cgcaccaaaa gttcgaaaaa ctgcggcgca gcagccaacg
     3301 catgcagagc tacatggccg acttcgaacg tcggcggaac agcacctatg tcacccgcat
     3361 gtgcgccgat cccgagtacc cgttcttgaa cgggctcctg gccgaggtcg cgcgcgctaa
     3421 agccaactgg cagcaagagc ttgcggccac tccgccgcca acggaagaac aagaggccct
     3481 ccgcgcagag ttgctcaccc aggccactca actctcgaat gtgctgcgcc aagcgcgggc
     3541 cctcgctgaa gccgcattga cgttcgaacc cgagcattgg gccgcgctcg cggccgagta
     3601 caagtcgcgc cgacgcatcg ctcgctgagc cgcttctcct cgagccccgc attctttccg
     3661 tgaactccga ccgcgctgcc ccggctcgcg cccgcgggcg gtgctaggac ccgtgtgccc
     3721 atggccgtca ccccgagtta cctcagctac gtcctcgatc agttttccgg agcgggcgaa
     3781 gtcgtgtcgc gccggatgtt tggcggagtg ggtctatacc gtgacggcgt gttcttcggc
     3841 gccgtcgacg acgacgccgt ctacatgcgc gtcgatgaag cgactcgccc cgagtacgag
     3901 cgccgtggca tgcccatgtt gcgccccctc aagagcaaac ccgatctcgt gatgcaggcc
     3961 tacttccagc taccgcccga gattctggat gatcgtgaag tgctgcacga atggctcgag
     4021 cgcgcggtgc gcgcggccgt cgcggccgcc aagcccaaac gcccaccctc tgcacgcacc
     4081 cgcaaaccca aggccgcttc cgcccgcaaa cgcacatagg ctgccgctga atgcgcgtgt
     4141 tcgagagggc ccgggccaca caacaacgtg cgcgcagctc gttactcatg agaagccagc
     4201 gcagccgcgg gccgacccga tctcagccag caccgcgccg gccctccttg tagacgc
//
LOCUS       DASTKU010000855         4528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1729963, whole genome shotgun sequence.
ACCESSION   DASTKU010000855 DASTKU010000000
VERSION     DASTKU010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729963"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFQ61_30080"
     CDS             complement(<1..298)
                     /locus_tag="VFQ61_30080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958794.1"
                     /translation="MHAHHALHLIIARHGQLEIRGAGEEESRPASGLLTAPGAEHAVD
                     ARGAEVLIVFLDPESEAGISLRKLIPGALRLLTEAECVRLRAGADPARIMRAGGA"
     gene            complement(464..952)
                     /locus_tag="VFQ61_30085"
     CDS             complement(464..952)
                     /locus_tag="VFQ61_30085"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9958795.1"
                     /translation="MVDLSSSPPNRHRELNLALEALHFAFRAVTEGPDAELAAHGLSR
                     VHHRILYFIAKTPRLRVSELLATLGVTKQAMNAPLRQLTERGLVAESVDSADRRGKRL
                     HLTAAGQSLERRLSGDQRAAFARVFRKVGKNKEQAWYEVMHLLVQNATGPSARKLDSP
                     TP"
     gene            1028..1402
                     /locus_tag="VFQ61_30090"
     CDS             1028..1402
                     /locus_tag="VFQ61_30090"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9958796.1"
                     /translation="MPVLDSATVGAFAAEWIAAWNARDVDRVLEHYAEDVRFSSPLVA
                     SIAGEPTGSLTGKPALRAYWSKALASLPELRFTLVDVLAGVNTVTLYYRGHRGTVAET
                     FHFDEAGRVRAANACYGLASAL"
     gene            1420..1893
                     /locus_tag="VFQ61_30095"
     CDS             1420..1893
                     /locus_tag="VFQ61_30095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9958797.1"
                     /translation="MSLKPPALDPSTVAPRTTSGYPEMFRPRVTPREKRALGDALGLT
                     RFGVNLTTLFPGKESSLRHHHAREDEFVFILEGEVVLRTENGEQVLCAGQCAGFPAGT
                     TDGHQLVNRSEQPARYLEIGNRDAADTVGYSDVDLACRKNAEGVWVYTHRDGTPY"
     gene            complement(1909..2856)
                     /locus_tag="VFQ61_30100"
     CDS             complement(1909..2856)
                     /locus_tag="VFQ61_30100"
                     /inference="COORDINATES: protein motif:HMM:NF018431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9958798.1"
                     /translation="MRESPPSPSLEALVRPRLQDLVRRALDTASAAERSQEDEPGAQL
                     VAPRPGLFVLRQHRRTTFQAAIYDPMVCLILQGCKEMTFGRRTFRLRAGECALVSHDL
                     PIVSRVLEVPYLVLLFRIEIDVLRGLYEDVGELATALPEARALEAHPVGAHLLEVLGR
                     YLTIGESTMDARVLGPMLLKEIHYRLLTAPFGNMLRSLMRHDSYASSIARAIAMLRRD
                     FRSPVVIEDLARSVGMSVSSFHKHFKAVTASSPAQYQKTLRLLEAQRMLTSGKATVTA
                     AAMEVGYESPSQFSREYSRKFGRPPNEDLARHKSRNSDS"
     gene            2968..4080
                     /locus_tag="VFQ61_30105"
     CDS             2968..4080
                     /locus_tag="VFQ61_30105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET9958799.1"
                     /translation="MTTTVICGPNVTSLPRAIAEGDRLWLDQLDFERATGWSWKPYGL
                     CRGDTCIPIPRGSERPSALGDLIDVAELWRSLAWPIVRNGSSHFWVLGEGARIRADSL
                     VSLEAPEFALPSIDGQLHRLSEFRGRKVFLVTWASWCGCRADLPLWQSLYESTRERGL
                     TVVALALDEPERARPWIDAASAAFPCLIDRDHLTAQLYNFVNVPQAVWIDEQGCLVRP
                     PETAGMTDSFRRMDRKDFTLPAAAQAERQAAKARYYDAVRDWATRGASSPYALDREAL
                     RRRLALPSSASAEAHAHFRLGQALLREGLDTEAATEFAEATRLHPESWAIWRQTAEKD
                     ASGLAVSDAFWRRVDALGDRPYYAPVDLGNPPEPMS"
     gene            complement(4229..>4528)
                     /locus_tag="VFQ61_30110"
     CDS             complement(4229..>4528)
                     /locus_tag="VFQ61_30110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HET9958800.1"
                     /translation="KDQGRFEATHSEADKMMFKVPGLRNIEKTAPYFHDGSAANLEDA
                     IQIMSSHQLGRELKPEQVKLIAAFLRTLTGELPTAQIQNPELPKSGPATPKPNPT"
BASE COUNT          807 a         1402 c         1461 g          858 t
ORIGIN      
        1 cggcacctcc tgcgcgcatg atccgcgcgg gatccgcgcc agccctgagc ctcacgcact
       61 cggcttcggt cagcaggcgg agcgcaccgg gaatcagctt gcggagtgaa atcccagctt
      121 cgctctccgg atcgagaaac acgatcagga cctcggcccc gcgtgcatcc acggcatgct
      181 cggcacccgg agccgtgagc aatccgcttg cggggcgcga ttcctcttcc cccgcgccgc
      241 ggatctcgag ttgcccatgg cgtgcgatga tcaggtgcag cgcgtggtgg gcgtgcatgt
      301 cgctttgcga gcccggtcca gctgtggcga tcaacggagg ccagatgggg gcttcgacgg
      361 gggaggtgcc aggccgcgga acgcgaggca agcgcatggt tgtcccgagg atatcccaag
      421 ccgggccgcg cccccaggcc cgtagccggg cgaagagggg gccctaaggg gtcggcgagt
      481 ccaatttccg cgcggagggt ccggtcgcgt tctgcacaag caggtgcatc acttcgtacc
      541 aggcctgctc cttgttcttg cccaccttgc ggaagacgcg cgcgaaagca gcgcgctggt
      601 cacccgataa gcgccgctcc aggctctgtc ccgcggcggt gagatgaagc cgcttgcctc
      661 ggcgatcggc ggagtcgacg ctctcggcga cgagaccgcg ttcggtcaac tggcggagcg
      721 gcgcgttcat ggcctgcttc gttacgccca aggtggccag cagttcactt acgcggagcc
      781 gcggtgtctt ggcgatgaag taaagaatgc ggtggtggac ccgcgacagc ccatgcgccg
      841 cgagctccgc atctggcccc tccgtgaccg cgcgaaacgc gaagtggagt gcctcgagag
      901 cgaggttgag ctcgcggtga cgatttgggg gagagcttga taggtcaacc atcttgactc
      961 attggaagag cgctgctacc gatcgtgaac gtaggtcaat tagtttgacc tataaaggag
     1021 cccggtcatg cccgttctcg attccgctac cgtaggcgcg ttcgcggccg agtggattgc
     1081 cgcttggaac gcccgcgacg tcgatcgggt cctcgagcac tatgcggaag acgttcggtt
     1141 ctcctcgccg ttggtcgcca gtatcgccgg cgaacccacc ggatccctga ctggaaagcc
     1201 cgcgttacgt gcctactgga gcaaagccct ggcctcacta cccgagctgc gcttcactct
     1261 ggtcgacgtg ctcgcggggg tgaataccgt aaccctctat tatcggggtc accgtggcac
     1321 cgttgccgaa acgttccact tcgacgaggc cggacgggtc cgggccgcca atgcctgtta
     1381 cgggctcgca agtgccctct gatggaggaa atcgaagcca tgtcgctcaa accgcccgcc
     1441 ctcgacccat ccaccgtcgc gccccgcacg acgtccggat atcccgaaat gttccgcccg
     1501 cgggtgacgc cgcgtgaaaa gcgcgcactg ggagacgcgc tcggtctcac gcgcttcggc
     1561 gtgaacttaa ccaccttgtt ccccggtaag gagtcttcgc tgcgtcacca tcacgctcgc
     1621 gaggacgagt tcgtgttcat cctcgagggt gaggtcgtgc tccgaacgga gaatggcgag
     1681 caagtactgt gcgccgggca gtgcgctggc tttcccgccg gcactacgga cgggcatcag
     1741 ctcgtcaatc gcagcgaaca gcctgctcgt tacctcgaga ttggcaatcg cgacgcagcg
     1801 gacaccgttg gttactcgga cgtcgacctc gcgtgccgca agaatgcgga gggggtgtgg
     1861 gtctacacac atcgcgacgg aactccgtat tgaacttgcg ggtctcgttc acgagtccga
     1921 gtttcgcgat ttgtgcctgg cgagatcctc gttcgggggc cggccaaact ttctcgaata
     1981 ctcgcggctg aattggctcg gactctcgta gcccacctcc attgcggcag ccgtgaccgt
     2041 ggctttgccg ctggtcagca tgcgctgcgc ctcgagcagc cgcagcgtct tttgatactg
     2101 ggccggagag gatgcggtca cggccttgaa gtgcttgtga aacgacgaga cactcattcc
     2161 cacgctgcgt gccagatctt ctatgaccac tggcgagcgg aaatcgcgcc gcaacattgc
     2221 gatggcgcga gcgatcgagc tcgcgtagct gtcgtgccgc attagactgc gcagcatgtt
     2281 gccgaacggc gcagtgagta agcgatagtg gatctccttg agtagcatgg ggccgagtac
     2341 ccgcgcatcc atggtcgatt caccgatcgt gagatagcgc cccagtactt ccagcaagtg
     2401 ggcgcctacg ggatgggcct cgagcgcccg tgcttccggc aaggccgtgg ccagctcccc
     2461 gacgtcttcg tagagaccac gtaatacgtc aatttcgatc cgaaagagca gcactaaata
     2521 cggcacctct agaacccggg acactatggg caaatcgtga ctcacgagcg cgcactcacc
     2581 cgcgcgcaaa cgaaaggttc gcctcccgaa cgtcatctct ttgcaaccct ggagaatcag
     2641 gcaaaccatc ggatcgtaga tggcagcctg gaacgtggtg cggcggtgct gccgcaagac
     2701 gaacagaccg ggccgaggag ctacgagctg ggccccgggt tcatcttcct gagaacgttc
     2761 agcggccgac gccgtatcga gtgcgcgacg gacgaggtcc tgcaagcgtg gacgcacgag
     2821 cgcttcgagt gaggggcttg gggggctctc gcgcatgtgc ctgattctgc caatcgaaga
     2881 gaattaggca agacacatgt agtcttgggc aagcgcgccc ggcggccgca gaccaagctg
     2941 tgaatcacag cctcccaagg agaagtgatg acgacgaccg tgatttgcgg cccaaacgtg
     3001 acctcattgc cccgcgccat cgccgagggc gaccgtctct ggctggacca gctggatttc
     3061 gaacgcgcta cgggctggag ttggaagcca tacggcttgt gtcgagggga cacttgcatt
     3121 cccattccgc ggggcagcga gcgcccgtcg gcgcttgggg atctcatcga tgttgccgaa
     3181 ctttggcgga gtcttgcttg gcccatcgtg cgaaatgggt cttcccattt ctgggtattg
     3241 ggtgaggggg cgaggattcg agccgattcg ctggtttccc tcgaagcccc tgagtttgca
     3301 ttgccgtcga tcgacgggca gcttcatcgg ctgtcggagt ttcgaggacg caaggtcttc
     3361 ctcgtgacgt gggcttcctg gtgcggatgc agagcagatc ttcccctgtg gcagtcgctc
     3421 tacgaaagca cacgcgagcg gggcttgacg gtcgtagcgc tcgcgctcga tgagcccgag
     3481 cgtgccagac cttggatcga cgcggcttca gcggcttttc catgtttgat tgatcgcgac
     3541 cacctgacgg ctcagctcta caatttcgtc aacgtgcccc aggccgtctg gatcgatgaa
     3601 caggggtgcc tggtgcgacc gcccgaaacg gccgggatga ctgacagctt ccgccgcatg
     3661 gaccgcaagg acttcacgct gcctgcggcg gcccaggcag agcgccaagc cgcgaaagcg
     3721 agatactacg atgccgtccg tgactgggcg acgcgcggcg cgtccagccc gtacgccctc
     3781 gaccgcgaag ccctgcgcag gcgcttggct cttcctagct cagcgagcgc agaggctcac
     3841 gctcactttc ggttggggca agcgttgctt cgggagggac tggacacaga agcagcgacg
     3901 gaattcgcgg aagccacccg actgcatcct gagtcgtggg caatctggcg ccagacagct
     3961 gaaaaggacg cgagcggtct cgccgtcagt gacgcttttt ggcgacgcgt cgatgcgctg
     4021 ggggaccgcc catactacgc gccagtggac ctggggaacc cgccagagcc aatgtcctag
     4081 gcgctgccga gatcccacag gcgatccagg ggacgatacc gcgtgtgata tgccgcgaac
     4141 gtcggccgac gtttcgcgca agccctacgt ccctgctcgc ggccattctt gggtttcttc
     4201 ggctctagcc gacgcgtgga gacagccctc aggtcgggtt cggcttgggc gtggccggac
     4261 ccgacttggg aagctccgga ttctgaatct gtgcagtggg gagctccccg gtcagggtgc
     4321 gtagaaatgc tgcgattagc ttgacctgct cgggcttcag ttccctgccg agctgatgcg
     4381 acgacatgat ctgaatggcg tcttccaagt tggcggcaga cccgtcgtgg aagtacgggg
     4441 ccgtcttctc gatgttacgc agtccgggaa ctttgaacat catcttgtcg gcctcgctgt
     4501 gggtcgcctc gaagcgtccc tgatcctt
//
LOCUS       DASTKU010000856         6868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_167276, whole genome shotgun sequence.
ACCESSION   DASTKU010000856 DASTKU010000000
VERSION     DASTKU010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167276"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /gene="dut"
                     /locus_tag="VFQ61_30115"
     CDS             <1..279
                     /gene="dut"
                     /locus_tag="VFQ61_30115"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET9958801.1"
                     /translation="IPEGYEGQVRPRSGLALKYGISIVNAPGTIDSDYRGEVSVLLIN
                     LGHEPFVIEPRARIAQLVICPVVRASLVEANTLSGTRRSSGGYGSTGV"
     gene            complement(258..1109)
                     /locus_tag="VFQ61_30120"
     CDS             complement(258..1109)
                     /locus_tag="VFQ61_30120"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9958802.1"
                     /translation="MASNAAKVELGALTGERSPAQLTLAGASPPVLGLHGFGGTPFEV
                     EGVIEAARRSGLAALCPLLPGHGKRSARALSVMRYSDWFSAAEQALLSLGEPAVIAGF
                     SMGSLLAMELAIRYPERVLGLVLMGNAAWLKQPFPGLALSLAERLRLPDFMLRKRGPD
                     IGDALARADHVSYNAHPVHAAIEVRRAGERLREELGKITCPTLVLHGAKDRVCPVENA
                     WRVAERLGTEDCRVVVFPRSRHVLMRDFERAAVREEIARFLRRWAPAGAEAEPENPAA
                     PQTPVEP"
     gene            complement(1084..2544)
                     /locus_tag="VFQ61_30125"
     CDS             complement(1084..2544)
                     /locus_tag="VFQ61_30125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9958803.1"
                     /translation="MADTREAAPLTQASPTVLVVDDEKNIRRTLEMVLSGEGYRVLEA
                     ESAERALELLRAPQLPVDVAIFDLKLPGMSGLDALERLRADDATRELPVIVISGHATV
                     HDAVSAIKLGASDFFEKPLNRERVLVSVRNSVRAARMSRTLEQMRGELEARYEMIGTS
                     PPIQKLFQDIDKVAPTKASVLITGESGTGKELVSRAIHRLSPRKEAPFVKVNCAAIPR
                     ELIESELFGHERGSFTGAQGRKRGFFEQAHGGTLFLDEIGDMDLAAQAKVLRALQNGE
                     IVRVGSEHVIHVDVRVLAATNKDLAKSVAQGTFREDLMFRLDVFPIRVPALRERPSDI
                     RLLADAFVAAFCNENGLRQKRIDPSVYEALTRRKWPGNVRELKNVVERAAILSSDVLT
                     IADLPEDPHSSPFEDETEGGAEPEEMSSNGSNFPEPSRSRLTLREYRDSAERAYIIET
                     LKELSWNISRAAVVLGVERTNLHKKIRAYGIKRGES"
     gene            complement(2615..3580)
                     /gene="bamD"
                     /locus_tag="VFQ61_30130"
     CDS             complement(2615..3580)
                     /gene="bamD"
                     /locus_tag="VFQ61_30130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HET9958804.1"
                     /translation="MSFLVSASYRVARGPKPSPSWLGSSASDSWSRLLRSIRRRGLVR
                     LLFLVGVAAASGSLPACQSELPKAKGALAYTDNARRLYERALRAAEDKNWENATRMFN
                     DVRREYSYSRYARLSELRLADLNYDQGKLAEATSAYKAFVHDYPNDPEVPYARFRIAK
                     SEYETVSQSFLLPPLEERDLASVHDALSTIRSYLADFPDAEHAEDLRYMLEVVLGLLA
                     RHELYVGRYYLDQDRLDAAAGRANYALENFPRSGLEAEALLLLAEIRMKQKKPEEARQ
                     YLEQLLKTYSESPFSVPARKYLQRLGPAEPTASKRQSRAADSARF"
     gene            3814..4293
                     /gene="greA"
                     /locus_tag="VFQ61_30135"
     CDS             3814..4293
                     /gene="greA"
                     /locus_tag="VFQ61_30135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET9958805.1"
                     /translation="MSEKVPVPMTPAGAQKLREELTRLKEERPKISREIGIAREHGDL
                     SENAEYHAAKERQGLVEARIKDLEDKLARAEVIDPTKLSGDRVRFGATVKLTNLDTDE
                     EQTFQIVGADEADIKKGTISISAPLSRALIGKTVGDEVVANLPGGTRRYEIGSIEFR"
     gene            4388..>6868
                     /locus_tag="VFQ61_30140"
     CDS             4388..>6868
                     /locus_tag="VFQ61_30140"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9958806.1"
                     /translation="MSPATHLLRRAASAAQAAAIFLLLELAAVALLHRRELTSAWELQ
                     RGSLWLAPSALALALIFGALGYALHGLWWRSAKPEARLSLALVLGALASSIAYGVGGG
                     RHLAAPGLRLGFALGVGVAAAAGAWLGAPRAAELARRKPERAAVSWALLTLLVALANW
                     LILPRLYPAFHWGLGGASLVLATWVSLTAERAASTLSRASRPTGAIGVLSLFALAACG
                     IAPAAERLAHFDNFRFLLLENAPVLGRLVELTARLAPTETVTAADAEASSGACSEFSA
                     CGPSASSASGSPGLNLAQHDILLVTVDALRADHVGAYGYKRPTTPHFDSLASEGIVFE
                     RAYTATPHTSYAVTSLMTGKYMRPLLLQGAGADSDTWAELLRTYGYRTAAFYPPAIFF
                     IDSERFEPFQKRNLGFEYAKIEFAEGNKRVAQVTDYLREAPRDVPLFLWVHLFGPHEP
                     YEAHPEHEFGQRDIDRYDSEIAAADETLGKIVEAFRKDRPRAAVLLTADHGEEFGDHG
                     GRYHGTTVYEEQLRVPLVIAAPGLLKARRIREVVQTVDLLPTLLGAMSIPRPPRVRGR
                     DLGPLLSGQRPEEPGLAYGETDEQSVLARGSLRLLCARRVGACKLYDLDRDPEQRRDA
                     SDDHAAEQLQLRTALRELGASHGRYESQGLRAEGKGFPAAILRGLSGDGDAAADVAAL
                     LDDADPAIRRKAAEVLWQLRRKESAGALRLALGRDDDLRVRQFAALALTRLGEGAPLV
                     LELLESSNLEQRRQAALALAESGDRRGLMLLLEEFREGKFQDLPHARDLLDAFATLRA
                     KEAVPALIPRLSDVRLRPYIAQALAKIGE"
BASE COUNT         1203 a         2255 c         2216 g         1194 t
ORIGIN      
        1 atcccagagg gctacgaggg gcaggtgcgg ccgcgctccg gtctggcgct aaagtacggg
       61 attagcatcg tgaacgcgcc cggcaccatc gactcggact atcgaggcga agtctcggtg
      121 ctgctgatca acctcgggca cgagcccttc gtgatcgagc cgcgggcgcg catcgcccag
      181 ctcgtgattt gcccggtggt ccgcgccagc ttggtagagg cgaacacgct ctctggaacg
      241 cgccgatctt cgggaggcta cggctcgacc ggcgtttgag gcgcggctgg gttttccggc
      301 tcggcctcag caccggcagg ggcccaacgg cgcaggaagc gggcaatctc ttcgcgcacg
      361 gcggcgcgct cgaaatcacg catcaacacg tgtcgagaac gcgggaacac caccacccgg
      421 cagtcttccg tacccagacg ctccgccacg cgccaggcat tttcgaccgg acagacgcga
      481 tctttggcgc cgtgcaacac caacgtcggg cacgtaatct tgcccagttc ctcgcgcagc
      541 cgctcacccg cgcgccggac ctcgattgcg gcgtggaccg ggtgcgcgtt gtaactgacg
      601 tgatccgccc gcgccaacgc atcgccgatg tccgggccgc gcttgcgcag catgaaatct
      661 ggcagacgca gccgctcagc caagctgagt gccaagcccg gaaacggttg cttgagccaa
      721 gcggcgttgc ccatgagcac caggcccagc acccgctcgg gatagcgaat cgcaagttcc
      781 atagccaaca ggctacccat cgaaaagccg gcaatcaccg cgggctcgcc gagcgagagc
      841 aaagcctgtt ccgccgcact gaaccagtcc gagtagcgca tcacggacag cgctcgggcg
      901 ctgcgcttgc cgtgcccggg aagcagcggg caaagcgcgg ccagcccact ccggcgcgca
      961 gcttcgatca cgccttctac ttcgaaagga gtgccgccga agccgtgcaa gccaagcacg
     1021 ggcggcgagg caccggccaa ggtgagctgc gcgggggagc gctccccggt gagcgcaccc
     1081 agctcaactt tcgccgcgtt tgatgccata tgcgcgaatc ttcttgtgca ggttggtgcg
     1141 ctctaccccg agcaccacgg cggcgcgcga gatgttccaa gacagctcct tcaaagtctc
     1201 gatgatgtac gcgcgttcgg cgctgtcgcg atactcacgc aaggtaagtc ggctgcgcga
     1261 aggctccggg aagttgctcc cgttgctgct catttcttcg ggctccgccc ccccttcggt
     1321 ctcgtcttcg aaggggctcg agtgcggatc ctcgggcaga tctgcgatcg tgagtacgtc
     1381 actcgaaagg atcgcggcgc gctcgaccac gttcttcagt tcgcgtacgt taccgggcca
     1441 cttgcggcgc gtgagcgctt cgtaaaccga cggatcgatt cgtttctgac gtagcccgtt
     1501 ctcgttgcag aacgccgcca cgaaagcatc cgcgaggagg cgaatgtccg aaggccgctc
     1561 ccgcaaagcc ggtacgcgga tcgggaagac atccaagcga aacatcaaat cttcgcgaaa
     1621 ggtgccctgc gccaccgatt tggccagatc cttgttggtc gccgccaaca cccgtacgtc
     1681 cacgtgaatt acgtgctccg acccgacccg cacgatctcg ccgttctgga gcgcgcgcag
     1741 caccttggcc tgtgcggcta ggtccatgtc gcctatttcg tccaagaaca gcgtgccacc
     1801 gtgagcctgc tcgaaaaaac cacgtttgcg gccctgtgca ccggtgaacg agccgcgctc
     1861 gtggccgaac aactcgctct cgatcagctc acgaggaatc gcggcgcagt tcaccttcac
     1921 gaacggagcc tcttttcgtg ggctcaggcg atgaatcgcc cggctcacca gctctttacc
     1981 ggtgccgctt tcaccagtga tgagcacgct ggccttggtg ggcgccacct tgtcgatgtc
     2041 ttggaacagc ttctggatcg gcgggctcgt gccgatcatc tcgtagcgcg cctcgagctc
     2101 gccgcgcatc tgctccagcg ttcggctcat gcgcgcggcg cgcaccgagt tgcgcacgct
     2161 gaccagcacg cgctcgcggt tcaggggctt ctcgaaaaag tccgaggcgc ccaacttgat
     2221 ggcgctgacg gcgtcgtgca ccgtcgcatg gccactgatc acgatgaccg ggagctcccg
     2281 ggtcgcgtcg tcggctcgga gacgttcgag cgcgtccaga ccgctcatgc ccggtagctt
     2341 caaatcgaag atcgcgacgt ccactggcag ctgcggcgcg cgcagcagtt ccaatgcgcg
     2401 ctctgcgctc tccgcctcga gcactcggta gccctcgccg gagagcacca tctcgagtgt
     2461 gcgccgaatg ttcttctcgt cgtcgacgac caggacggtc ggcgacgcct gggtcagagg
     2521 agctgcttca cgcgtgtccg ccatacaatc cgccacaaga tatcaaaacc gccccgagaa
     2581 ccagcaactt tgctggctcc cgcgcccaaa tggctcagaa ccgcgcagaa tccgcggccc
     2641 gggactggcg cttactggcg gtcggctcgg cgggaccgag gcgctgaagg tactttcgag
     2701 cgggcacgct gaaggggctc tccgagtacg tcttcagcaa ctgttccaaa tattgccgcg
     2761 cctcctccgg cttcttctgc ttcattcgga tctcggccaa taggagcagt gcctccgcct
     2821 cgaggccaga gcgcggaaaa ttctccagcg cgtagttggc gcgcccggcc gctgcgtcca
     2881 ggcggtcctg atccaggtag tagcgcccca cgtacagctc gtgacgcgcg agcagcccga
     2941 gcaccacctc gagcatgtac cgtagatctt cggcgtgctc ggcgtccgga aagtccgcga
     3001 ggtagctgcg aatggtactc agggcgtcgt ggaccgaggc gagatcccgc tcctcgagcg
     3061 ggggcaatag gaaggactgg ctaaccgtct cgtattcgga tttcgcgatc cggaagcgcg
     3121 cgtaaggcac ctcagggtcg ttcgggtaat cgtggacgaa agccttgtaa gcgctggtcg
     3181 cttcggcgag tttgccttga tcgtagttga gatctgccag ccgaagctcg gacagccgcg
     3241 cgtagcggct gtaggaatat tcgcggcgca cgtcattgaa catacgcgtc gcgttttccc
     3301 aatttttgtc ctcggctgcg cgcaacgccc gctcgtagag gcgccgcgcg ttgtcggtgt
     3361 atgcgagtgc acctttcgcc ttcggtagct cggactggca tgccgggagt gacccgcttg
     3421 ccgcggccac accgactagg aacagcaagc ggaccaagcc tcgccggcgg atcgagcgca
     3481 gcagacggct ccaggaatcg gacgccgagc tgcccaacca ggacgggctc ggcttcggac
     3541 cgcgcgcgac gcggtagctc gcagacacca aaaaactcat cgagggcccc gggcagtagc
     3601 cggttcgcgc agcgagggca agcaccacgc ttcgatcggg gctcggcagg ggccccgcta
     3661 aacgcgccgc cgaggcgcgg cagcgatccc gcccgccccg aaagccagat agcccggcaa
     3721 agagtgaccc ctctcggcgg gcgtgaccga acttggggct cgcctttggc atccgacgaa
     3781 tcgtgagtaa tataggcgac ccgaggcgac gttatgagcg aaaaggtacc cgtcccgatg
     3841 accccggcag gggctcaaaa gctgcgtgaa gagctgacgc gcctcaaaga ggagcgcccc
     3901 aaaatctccc gcgaaatcgg catcgcccgc gagcacgggg atctgagtga gaacgccgaa
     3961 taccacgcag caaaagaacg tcaggggctg gtcgaggccc gcatcaagga cctcgaagac
     4021 aagctcgccc gggcggaggt gatcgatccc accaagctca gcggggaccg cgtgcgcttc
     4081 ggggccacgg tgaagctcac caacctggac actgatgaag agcagacctt ccagatcgtc
     4141 ggtgcggacg aagcggacat caagaagggc acgatatcga tctcggcacc gctctcacgc
     4201 gcgctgatcg gcaagaccgt cggagacgaa gtggtggcga atctgccggg gggcacgcgt
     4261 cgttacgaga ttggctcgat cgagttccgg tgacatggcc gcggccggtg gtgagccggc
     4321 cagcggtggt gcgcgcgccc aggccagctc agggcatgcg agctcagtgg gcccgagctc
     4381 agcacgcgtg agcccggcca cacacttact gcggcgtgcg gcctccgctg ctcaagccgc
     4441 agcgatcttt ctgttgctcg agctcgcggc cgttgccttg ctgcaccggc gtgagctcac
     4501 ctccgcttgg gagctgcaac gcggctcgct ttggctcgcc ccgtcagcgc tggctttggc
     4561 cctgattttc ggcgcgctcg gttacgcttt gcatgggctc tggtggcgtt cggcaaagcc
     4621 tgaagcacga ctttctctcg cgctggtgct gggcgcgctg gcgtcctcga tcgcttacgg
     4681 ggttggcggc gggcgacatt tggcggcgcc cggcctgcgt ctgggtttcg cgcttggagt
     4741 cggagtcgcg gccgcagcgg gagcgtggct gggggctccg cgcgcggcgg agctcgcgcg
     4801 gcgcaaaccg gagcgcgcgg ccgtgtcgtg ggctttgctc acattgttgg tcgcactcgc
     4861 caactggcta atcttgccgc ggctgtaccc agcctttcat tgggggctcg gtggcgcgag
     4921 cctcgtgttg gccacatggg tctcgcttac cgccgaacgt gccgcgtcca cgctctcccg
     4981 cgcgagtaga ccgaccgggg cgattggcgt tctatctcta ttcgcgctgg cagcgtgcgg
     5041 catcgcgccc gcggccgaac ggctcgcgca cttcgataac tttcgtttct tgttgctcga
     5101 gaatgccccg gtgctgggcc gcctggtaga actcacggcc cggctcgcgc cgacagaaac
     5161 ggtgacggcg gcggacgccg aggctagctc cggtgcctgc tcagagttct ccgcctgtgg
     5221 tccctccgcc tcgagcgcaa gcggcagccc cggactgaac ctcgcgcaac acgacatctt
     5281 gctcgtcacg gtggacgcgc tgcgcgccga ccacgtgggc gcctacggtt acaagcggcc
     5341 caccacgcct catttcgata gcttggcgag cgaagggatc gtcttcgaac gcgcgtacac
     5401 ggccacgccc cacacctctt acgcggtcac ctcgctcatg acgggcaagt acatgcgccc
     5461 gttgctgctg cagggggctg gcgccgattc ggacacctgg gccgaactat tgcggacgta
     5521 cgggtaccgc accgcagcct tctatcctcc cgcgattttc tttatcgatt cagagcgttt
     5581 cgagccattt caaaagcgga atctaggctt cgaatacgcc aagatcgagt tcgcggaggg
     5641 caataaacgt gtcgcgcaag tcactgatta tttgagggaa gcgccacgtg atgtgcctct
     5701 gttcctgtgg gtccacctgt tcgggccgca cgagccctac gaagcgcacc ccgaacacga
     5761 gttcggccaa cgagacatcg atcgctacga ctccgagatc gccgcggccg acgagacgct
     5821 cgggaagatc gtcgaggcct ttcggaagga tcgccctcgc gctgccgtcc tgctcactgc
     5881 ggaccacggg gaggagttcg gtgaccacgg cgggcgctac cacggcacca cggtgtacga
     5941 ggagcagctg cgggttccgc tggtgatcgc ggccccaggt ctgctcaagg cgcgaaggat
     6001 ccgcgaggtc gtgcaaacag tggatttgct gcctacgctg ctcggcgcga tgtccattcc
     6061 tcgtccgcct cgggtgagag ggcgggactt ggggccgctc ctcagcggcc agcgcccaga
     6121 agaaccaggg ctcgcgtacg gcgagaccga tgaacaatcc gtcttggcgc ggggttcgct
     6181 tcgattgctg tgcgcacgcc gggtgggcgc gtgcaagctc tacgacctcg atcgcgaccc
     6241 cgagcagcgc cgggacgcca gcgacgatca cgccgccgag cagctccaac ttcgaaccgc
     6301 attgcgcgag ctgggcgcct cccacggtcg ttacgagagt caaggactga gggcagaggg
     6361 caaaggattt ccagccgcga ttcttcgcgg gctgtccggg gacggggacg cggccgcgga
     6421 tgtggctgcg ctcctcgatg acgccgatcc ggcgatccgg cgtaaagctg ccgaggtgct
     6481 ctggcaattg cgccgcaagg aatcagcggg ggccttgcgg ctggcgctcg ggcgggatga
     6541 cgaccttcga gttcgacagt ttgcggccct ggccctgacc cgactgggag aaggcgcacc
     6601 cttagttcta gagctgctcg aaagctctaa cctggagcag cgacggcagg ccgcgctggc
     6661 attggccgaa agtggagacc gacgcgggct catgttgctc ttggaagagt tccgggaggg
     6721 caaattccaa gatctgccgc acgcgcgcga tttactcgat gctttcgcga cgctccgcgc
     6781 gaaagaagcg gtaccggcac tcatcccgcg cctctcggac gtacgcttga ggccatacat
     6841 tgcgcaggcc ctggccaaaa tcggcgaa
//
LOCUS       DASTKU010000857         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_389613, whole genome shotgun sequence.
ACCESSION   DASTKU010000857 DASTKU010000000
VERSION     DASTKU010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389613"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2019)
                     /locus_tag="VFQ61_30145"
     CDS             complement(<1..>2019)
                     /locus_tag="VFQ61_30145"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9958807.1"
                     /translation="GPEVFAFADKSALTPLDDFAQGTVVNAERFLPGYWDMGVHEGRL
                     YGVPAVPAVTGLYWNKRLFREAGLDPERPPRTLPELDEFARRLTRVQDGKITQAGFLP
                     SQPAGWFPFMWPYFFGGKLWDGGSHLTLNDAANIRAFEWVQGYAKFHGVAALQNMSAG
                     FGSSASAQDPFLSGKLGMVFQGVWLPSYIDRFAPNLEWGAAPFPGLDAAGAPIAYIDA
                     DMLVIPRGSRHAREAFEFMRYVARTEVMEKLCRLQHKNSPLREVTPSFFTGHPHREIR
                     MFQALAASPAAVSAPKLPIWAEYRAEIVALFQRVWLVQASPQQALDEAQGRLQRSLDR
                     ARARRAIPPRPALSWAPWGLLAFVFTGALAFAWWRRGHWWSVTKRRSPRSNASLPRGL
                     AFFSPWAVGFLVFTAYPIAASLIYSLCDYSALSPPKFVGLDNYAELLSDRLFLIALKN
                     TLTYVGLALPLGLGSSFAVALLLDAKVRGSSVYRTLVFLPSLTPVVVSSMVWLWIFNA
                     QYGVLNQLLGKLSFGLLGPVAWLSDPHTALPSLVLMSLWGGGHTVVVLLAAMQDVPAA
                     MYEAADIDGASFWHKVRHITLPLTSPVLYFNGIMGIISGLQIFAQPYIMTGGGPARAT
                     MTYAMRLYENAFAFLRMGYAAAMAWILFLLVLLLTWLAVRVASRRVHYT"
BASE COUNT          418 a          613 c          685 g          303 t
ORIGIN      
        1 gtgtaatgga cgcggcggct cgcgacacgc accgcgagcc aggtcagtaa gagcacgagc
       61 aggaacaaga tccacgccat cgcggcggcg tagcccatgc gtaagaaggc gaaggcgttc
      121 tcatacaagc gcatcgcgta ggtcatggtg gcgcgcgcgg gcccgccgcc ggtcatgatg
      181 tacggctggg cgaagatttg gagcccggag atgatgccca tgatgccgtt gaagtagagc
      241 accggtgagg tgagcggcag cgtgatgtgc ctcactttgt gccagaagct cgcgccgtcg
      301 atatcagcgg cttcgtacat ggcggcgggc acatcttgca ttgccgccag gagcacgacc
      361 accgtgtgtc cccctcccca caagctcatc aacaccagcg atggtagcgc agtgtgcgga
      421 tccgatagcc aagcgacggg tccgaggagg ccaaagctga gcttaccgag cagctgattc
      481 aatacgccat actgggcgtt gaagatccaa agccaaacca tggaactcac cacgacgggg
      541 gtgagcgaag gcaagaagac cagcgtgcga tacacactcg agccacgcac cttggcatcg
      601 agcagcaacg cgacggcgaa gctcgagccg agacccaagg gcaaagccag acccacgtac
      661 gtgagcgtgt tcttcagcgc gatcagaaac agtcgatcgg agagcaactc agcgtagttg
      721 tccaagccga cgaatttggg cggagagagc gcggagtagt cacacaggct gtaaatcaga
      781 gatgcggcaa tcgggtacgc ggtgaaaacc aggaagccca ccgcccacgg tgagaagaac
      841 gcgaggccgc gcggcagcga ggcgttgctg cggggtgatc ggcgtttcgt gacggaccac
      901 caatggccgc ggcgccacca cgcgaacgcg agcgcgcccg tgaacacgaa tgcgagtaac
      961 ccccatggcg cccagctcag ggccggtcgc ggcggaatcg cacggcgagc gcgggcacgg
     1021 tccaggctgc gttggagtcg gccctgggct tcgtccagag cttgctgcgg gctggcttgt
     1081 acgagccaga cgcgctggaa cagagcaacg atctccgcgc ggtattcggc ccagatcggc
     1141 agttttggtg cgctcacggc ggcggggctc gcggccagcg cttggaacat gcggatttcg
     1201 cggtgaggat ggccggtgaa gaagctgggc gtcacctcgc gcagcggcga gtttttgtgc
     1261 tgcagtcggc acagcttttc catcacctcg gtgcgcgcca cgtaacgcat gaactcgaac
     1321 gcctcccgcg cgtggcgcga cccgcgcggg atcacgagca tgtccgcgtc gatataagcg
     1381 atcggggctc cggcggcgtc gagacctgga aaaggggccg cgccccactc gaggttgggc
     1441 gcgaaacggt cgatgtagct cggtaaccaa accccctgga aaaccatgcc gagcttgccg
     1501 ctcaggaacg ggtcctgcgc ggacgccgag ctgccgaagc cggcgctcat gttctggagc
     1561 gccgccacgc cgtggaactt ggcgtagccc tgcacccact cgaaggcgcg gatattggcg
     1621 gcatcattca gagtgaggtg ggagccgccg tcccacagtt tgccgccgaa gaagtacggc
     1681 cacatgaagg ggaaccaacc cgcgggttgc gacgggagaa agcccgcctg cgtgatcttg
     1741 ccgtcctgaa cgcgagtcag tcggcgggcg aactcatcca gctcggggag agttcggggc
     1801 gggcgctccg gatccagccc cgcttctcgg aagaggcgtt tgttccaata caggccggtg
     1861 accgcgggca cggcgggtac gccgtaaagc cgaccttcgt ggacgcccat gtcccaataa
     1921 ccgggtaaga agcgttcggc gttgacgacg gttccctgcg cgaagtcgtc caggggggta
     1981 agcgcgctct tgtccgcaaa cgcaaaaacc tcgggaccc
//
LOCUS       DASTKU010000858        10962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_613357, whole genome shotgun sequence.
ACCESSION   DASTKU010000858 DASTKU010000000
VERSION     DASTKU010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10962
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613357"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFQ61_30150"
     CDS             complement(<1..479)
                     /locus_tag="VFQ61_30150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9958808.1"
                     /translation="MSLFSADVRRNPFPTYEQLRRDCPVLRDPRADLWLLFDHDSVRR
                     ALTDHETFSSAVIPGGVVSKWLIFLDPPRHARLRSVILRAFTPRAVADLEPRIAQLSH
                     DLLLPILERGGCDLVSEYAVPLPLLVIAELLGAPASDYRLFREWSDVILGLIHTVTA"
     gene            513..1280
                     /locus_tag="VFQ61_30155"
     CDS             513..1280
                     /locus_tag="VFQ61_30155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9958809.1"
                     /translation="MNESRILSVVGNDLGVAAGCGMYSSLHAPHGIELCIGLERELSV
                     RTGDGGSVRGRVIAVPPDLDRRIESEGVTLAMLYDVEPFPSLAGFARRTESPIVLDGP
                     LARRVIDAARAQRDSLFRAEVLRGLAEELAGYFSGERPRRIDPRVGRAVEALRAHDLR
                     RGEIIAASGLSEPHLQALFVRDIGISMRRFRLWQRMLSAVIALRRVDCTRAAHDAGFA
                     DLAHFSRTCARLLGYPPRTMRAAFTGELLRLPARVEW"
     gene            1887..2249
                     /locus_tag="VFQ61_30160"
     CDS             1887..2249
                     /locus_tag="VFQ61_30160"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET9958810.1"
                     /translation="MPLLSSPTARTPSNVLDQLDGKLANSVVVPPEQVGPDLVTMNSK
                     VLLACPEWGARREYQLVYPNAYNGSSGIVRVLSVLGAELLAARVGARFAIGTGANRRE
                     IELVGLSYQPESAGHWTL"
     gene            2288..3109
                     /locus_tag="VFQ61_30165"
     CDS             2288..3109
                     /locus_tag="VFQ61_30165"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET9958811.1"
                     /translation="MQQWKNVLVHADASVDRRGAIQQAFEIAEACEGRLTLFESISTG
                     YVDGHPDSTRALLKYAARTSADLIVGTASRTDIRELTPRGASALQLLRSSPVPVWLCA
                     PAAVRAARVVAAVDLSTIGADSRKLNEEVALAAARLSLIHHAELHVVCVADAARDRIF
                     RSIMRPAQYSRHLAQHRAELRAALDELVTRIGARPIAHLIEGDAVEALDRLLVDLRAD
                     MLVLGRDNSNPQGVLRSDLAEQLLCRADRSLLLVHSTRAPARVDQSVETRSGARV"
     gene            complement(3168..4604)
                     /locus_tag="VFQ61_30170"
     CDS             complement(3168..4604)
                     /locus_tag="VFQ61_30170"
                     /inference="COORDINATES: protein motif:HMM:NF016345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HET9958812.1"
                     /translation="MFHRSPRTSAALSLSILAACAACSAQDRSVSAQDSPSEELNHTT
                     APLSSAGPANLKAFVGGNDIALTWNAYPEFTVAYYRIFRDGREMGRAWPRRTHAVAFA
                     GFADPSVERGITYSYQVQAVGTAGETSPLSSSVSATHPTNGFPVPAILIDDSAAPQLR
                     PLLNKGADALRTWYPKIADLIAVGGYTPSKFLTLRAMVNRYPGEDSSVNHCGGAGWAD
                     GGDVLKICDYMANDDEGDMGLFVHEATHILQNYHSGAPKAVDEGIATWAGDLSQGIVR
                     SLDFRNLSYMNGYEYSAAFYGWIQSHVSPSFVRNMNIRAHNAEFKYEWYKLETGQDVN
                     QLWRAMTQVSLTMPFELRTFASLCAVPQDGKVKLEACRTEDQAKYFVYDPQLKRLYMN
                     AHCLTVTNDGQVTDTTCGARKLDGSEQELPANAQSWTYTGTTWANVGTSQCLQPQGPT
                     FLAGTPLVTAPCSSASKWSAPAAPLSSN"
     gene            complement(4838..6523)
                     /locus_tag="VFQ61_30175"
     CDS             complement(4838..6523)
                     /locus_tag="VFQ61_30175"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HET9958813.1"
                     /translation="MLSESRLYLVFGYVALFGACSSASDTLDSPVSPASGGHVSSAGG
                     PGNSAQGGHTVAVGGQSSGYTSAPSGFGGTASGGLPSSWNGGASSKGGSGNSANGGSP
                     GGQAGQSTSAGGSSTNAGNASSGGTSSAMGGQTSGGSGPMSGGSPSSGGAPEPRPSAQ
                     QVIGYFAQWGIYQRNYRVKNIVDSGSAKTLTAINYAFGNIVNGECVMVTRAGVGDAFA
                     DYQKSFQATESVDGKADTWDQNLKGNFNQLKKLKALYPNIKVLISLGGWTWSKGFSDA
                     ALTPESRARVAKSCVDLYIRGNLPMLDGDPAGGAAAAAGVFDGVDIDWEYPAYAGDVG
                     SVYRDEDTRNFTLLLEEFRRQLSAIDPKLLLTIAAPAGSPQTALLELGQIHRHLDWIN
                     LMSYDMHGAWDATGPTNFHAPLLLSNKDPARESGLSADAAIQRYLDGGVPASKLTLGL
                     PFYGRGWTGVAAANNGLYQSASGPAPGTYEAGIEDYRVLAARAKPGFRDSAAGAYWTF
                     DGNEFWSFDDPENIRSKTEYARSKGLGGVMAWSLDGDTADGQLMRAIAAGLSP"
     gene            complement(6609..7025)
                     /locus_tag="VFQ61_30180"
     CDS             complement(6609..7025)
                     /locus_tag="VFQ61_30180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958814.1"
                     /translation="MTTLDSVASPSAAGAKLWAITGEFAPALWQRSGRLRFGLGLALQ
                     VGWIHASSFGIRAPRAANAPWYAVAPALMGQLEVSPRVHLRLSIEGTVALGRPRFEID
                     GVGVVYEAPRFVPILGLSAGFDVQPVAAPRVTVPPQ"
     gene            complement(7754..8536)
                     /locus_tag="VFQ61_30185"
     CDS             complement(7754..8536)
                     /locus_tag="VFQ61_30185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958815.1"
                     /translation="MTELDPDVSAQLRSAYQARAPSSDRKAKMLRTLQASLIAGGLTA
                     GGAAQATQSSLKLWLSKHALTFSLLGLGAGSAALGGVWISRAVSPPAATAVVSSAAPH
                     EDRSPAGSPPSRDEASTLDAPVLASSGNPTSPREQTPVAPAPQTRDSISRTLPDALPR
                     NATSSRNTPAPRSEVVPKDDLAAEVALLQKANAAYRSGRAADALTLVSEHVKRFPRSQ
                     LSAERSTLKVLALCASHRVEEARRAASGISGSAALRGSCIDR"
     gene            complement(8533..9075)
                     /locus_tag="VFQ61_30190"
     CDS             complement(8533..9075)
                     /locus_tag="VFQ61_30190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9958816.1"
                     /translation="MVARPASEPAPSFETLYEEHVDFVWRYAATHGIAPAQLDDVVQE
                     VFLVVHARLASFERRSGVKTWIAGIAVNVVRGFRRRRRNRQLGEPIEELESFRSPEGT
                     ACDALELKRTLQLLDRALAQMAELPREAFVLCDLEQLPQVEVAAMLGVNENTLRARLR
                     TARLIVKEVAGSAAPGGGSP"
     gene            complement(10177..>10962)
                     /locus_tag="VFQ61_30195"
     CDS             complement(10177..>10962)
                     /locus_tag="VFQ61_30195"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9958817.1"
                     /translation="HNGRVIWLFGMQARRTPSAHPPDYREEDFPVEERLGEDILQRWI
                     MELLRPLIERWLVTRGSPSFVGADQFIYFDARQPAKRVAPDIYVLPGVALDTDVQSWK
                     TWEARVVPSFALEVCSQDWRKDYEAAPALHEQIGTQELIIFDPRYEARSDGLRFQRYE
                     RVGDRLLRRETTSEDRVPSRSLGCYLRLVGSGMHQRLRLALHPDGEHLFPTGEEAERL
                     AKEAERLAKEAERQAKEAERQAKEAALARVAELEALLKAQTSR"
BASE COUNT         1933 a         3349 c         3529 g         2151 t
ORIGIN      
        1 tcagccgtta ccgtgtgaat gagccccaaa atcacgtcgc tccactcccg aaaaagtcgg
       61 tagtccgacg ccggggcacc gagcaactcg gcgatgacca gcaagggcag gggaaccgcg
      121 tattcactga ccagatcaca gccaccgcgc tcgaggatcg gcagcagcaa gtcgtgcgac
      181 aattgcgcga tgcgcggctc gagatcggcc accgcgcgcg gggtgaaagc gcgcaagatt
      241 acggaacgca aacgggcgtg gcgtggtgga tccagaaaga tgagccactt gctcacgacg
      301 ccccctggga tcactgccga gctgaaggtc tcgtggtccg tcagcgcgcg ccgcaccgaa
      361 tcgtggtcga acaacagcca gaggtccgct cgagggtcac ggagcacggg gcagtcgcgg
      421 cggagctgct catacgtggg aaatgggttt cgtcgcacat cggcagagaa cagactcatg
      481 gtcttggtgt aagctgagcc ccggagcctc gcttgaacga atctcggata ctgtcggtgg
      541 tcggcaacga tttgggcgtc gcggcgggat gcggcatgta ctccagcctg cacgcgcccc
      601 acggaatcga gctctgcatc ggactcgaac gagaactcag cgtccgtacc ggggacgggg
      661 gctcggtgcg cggacgagtc atcgccgtgc cgcccgatct ggatcgccgg atcgaatcag
      721 agggggtcac tcttgcgatg ttgtacgatg tggagccttt cccgtcactt gccggcttcg
      781 cgcggcgaac ggaaagcccc atcgtgctcg acgggccctt ggctcgccgc gtgattgatg
      841 cagcgcgggc tcaacgcgac tccttatttc gagcggaagt actgcgcggg ttggctgagg
      901 aattggcggg ttacttttcc ggtgagcgcc cgcggcggat cgatccccgt gtcggacgcg
      961 ccgtggaggc attgcgagct cacgacctgc ggcgaggcga aatcattgcc gcgagtggtt
     1021 tgtcagaacc ccacttgcag gcactgttcg tgcgtgacat cggcatcagc atgcgtcgct
     1081 tccggctgtg gcaacgtatg ctgagcgcgg tcatcgccct ccgtcgtgtg gactgcacgc
     1141 gggcggcaca cgacgctggg tttgccgatc tcgcccactt ctcccggact tgtgcacgtt
     1201 tgctcgggta tccgccacgg accatgcgcg cggccttcac cggagagctt ctgcggcttc
     1261 ccgcgcgcgt cgagtggtga acgcagcttt cctcggcgct ggtaaacccg gcgaagcttc
     1321 ttcagggcgc tgaataactt tatatccaga tgaccgtccc gcagccgggt gctgggtagg
     1381 tcaaaggcga tcaccctcga gaaacgatga atccgtgcgc tactatcgtt tgacggagac
     1441 accgcggccg cgttgcagag tgcggccagc gcggtatttc gacgggagta gggcgatatg
     1501 gctctgcgcc ctcgagtctc gtgacgggct tcgcgcccgt ggcatcctgg ccgaccgcag
     1561 gagctggggg cctctgcctt gacgcgcgtg ggtttagctc tagactagcc ggcattatgg
     1621 ttggttgacg ttgccattgc ggtttcgcga tttgctcctg cgcaggtcgc gttgaatgcg
     1681 ccgcaatgag ccaacgccct ccagtagatg ccttcatggt cggaagttct ggcgtcgtct
     1741 acgccggggc cggtacttca ggcccagcaa ccgcggatta ggctgaaaag ctctctcgcc
     1801 gttacgcaga gcgtccgttt ggctcgatga ggtgtttggt catggattca aggtccatat
     1861 tgtctgtcgt cgattattct cggctgatgc cacttttgag ctctcctacg gctcgaaccc
     1921 ccagcaatgt gctcgatcag ctggacggaa agctcgcaaa ttccgtcgtt gtcccgcccg
     1981 agcaggttgg tcccgacctg gtgacgatga actccaaggt tctgctcgct tgtccggagt
     2041 ggggagctag gcgcgaatat cagctggttt atccaaatgc atataatggt tcgtcaggaa
     2101 tagttcgtgt gttgtccgtg ttgggtgcag agctcttggc ggcgcgagtg ggcgctcgtt
     2161 tcgcgatagg aacgggcgcg aatcggcgtg aaatcgagct cgtcggtttg agttatcaac
     2221 ctgagtccgc tgggcactgg acgttgtaat cctcgtcgca cagcaactcg tagcagagga
     2281 gagagccatg cagcagtgga agaacgtgct cgttcacgcg gatgcaagtg tggaccggcg
     2341 tggcgcaatt cagcaggcgt tcgaaatcgc ggaggcgtgc gaaggtcgcc tcacgttgtt
     2401 cgagagtatc tcgacgggtt acgttgacgg tcacccggat tcgacgcgcg ccttactcaa
     2461 gtatgcggca cgcacctccg ccgacctgat tgttggcacc gcgtcgagga ccgatattcg
     2521 cgagctgacc ccgcgggggg cgagtgcgct ccagctcctg cggtccagtc cggtgcccgt
     2581 ttggctctgc gctcctgctg cggtccgggc cgctcgagtg gtggcggccg tggatctgtc
     2641 caccattggc gcagattcgc ggaagctgaa cgaggaggtt gcgttggcgg ccgcacgcct
     2701 gagcctgatt caccacgccg aactgcacgt ggtgtgcgtc gcggatgcgg cgcgagatcg
     2761 gatatttcgg agcatcatgc gtccggcaca gtacagccgt catctggcgc aacatcgggc
     2821 cgagctccga gccgcgttgg acgagctggt gactcggatc ggcgcccggc ccatcgctca
     2881 cttgattgag ggagacgcgg tggaagctct agatcgtttg ctcgttgatt tgcgtgcaga
     2941 catgctcgtt ctcggtcgcg ataacagcaa cccgcagggc gtgctccgtt ccgatctggc
     3001 cgagcagctc ttgtgtcgag ccgatcgttc gctcctgctg gtccactcga cccgagctcc
     3061 cgcacgcgtc gatcaatcgg tcgagacgcg ttccggcgct cgggtgtgag gccatccgcc
     3121 cgcagcgcgc tggcggatga gctcattacc gcgtgaggtg ctcgacgtca gtttgacgag
     3181 aggggagcgg ctggagccga ccacttggac gcactgctgc acggtgccgt caccagagga
     3241 gtgcccgcca ggaaagtagg cccctgaggc tgcaaacatt gggacgtacc gacgttagcc
     3301 caggtagtac cagtatacgt ccagctttgc gcgttcgcag ggagctcctg ctcgctccca
     3361 tcgagcttcc ttgcgccgca cgtcgtgtca gtcacttgac cgtcgttcgt gaccgtcaag
     3421 caatgggcat tcatatacaa acgtttgagc tgcgggtcat agacgaagta cttggcctga
     3481 tcttcggtgc ggcaagcctc gagcttcact ttgccatcct ggggtaccgc gcagaggcta
     3541 gcgaaggtgc gaagttcgaa gggcatagtc agagagactt gggtcatggc gcgccacaac
     3601 tgattcacgt cttggccggt ctcgagtttg taccactcgt acttgaactc cgcattatgg
     3661 gctcggatgt tcatatttcg aacgaagcta gggctgacgt gagactgtat ccagccgtaa
     3721 aacgcggccg aatactcgta gccgttcatg taagataggt ttctgaaatc gaggcttctt
     3781 acgatgcctt ggctcagatc gcccgcccaa gtggcgattc cttcgtctac ggctttcgga
     3841 gcgcccgaat ggtagttctg cagaatgtga gttgcctcgt ggacgaatag gcccatatcg
     3901 ccttcgtcgt cattggccat gtagtcgcaa atcttgagaa cgtcaccacc atccgcccag
     3961 cccgcgccgc cgcagtgatt cactgaagaa tcttcgcccg ggtatcggtt gaccatcgct
     4021 cggagggtca ggaatttgct cggtgtgtat ccgccgacgg cgatgagatc cgcaattttc
     4081 gggtaccaag ttcgcagtgc gtccgcaccc ttgttgagta gagggcgaag ttggggcgcc
     4141 gcagagtcgt cgatgagaat ggccggtacc ggaaaaccgt tggtagggtg cgtggcggat
     4201 acgctgctgc tcagagggga ggtctctccc gcggttccca cggcttgtac ttggtaactg
     4261 tacgtgatcc ctctttctac actcggatcc gcgaagcccg cgaaggcaac cgcatgcgtc
     4321 cggcgtggcc acgcgcggcc catctcccgg ccgtccctaa atatgcggta gtatgcaacc
     4381 gtaaactcag ggtaagcgtt ccatgtcaac gcaatatcgt tcccgccaac gaaggctttg
     4441 agattggcag ggccggcact ggagagcggg gcggtggtgt ggttgagttc ttcactcggc
     4501 gagtcctgcg cagagaccga gcggtcctgc gcggagcaag cggcacatgc cgctaggatg
     4561 gagagagaaa gggccgcgct cgttctgggc gatcggtgaa acatgggcac tcctgatgaa
     4621 atgggagcgg gctcgggtcg cccgccggag cgcgccttga gcaagtgacg tgcccaaaag
     4681 cgcccgttga gcggaaccct ggatagcgcc gatgttttgt cggttcgacg gcacgaggtc
     4741 ggtgattgtg ccgtcgttca cgatggtttg gaaagcctag tggaacacgc tgtaacgttg
     4801 cgaggtgttg cgttcgcgtc cgcgtcgttc gtcagggtca gggacttagt ccggccgcga
     4861 ttgcgcgcat gagttggccg tcggcggtgt caccgtcgag cgaccaagcc atgacgccgc
     4921 ccaggccttt cgatcgcgcg tactcggtct tgctacggat gttctcggga tcgtcgaagc
     4981 tccagaactc gttgccgtcg aaggtccaat aagcgcccgc ggcgctgtcc cgaaagcccg
     5041 gcttggcgcg cgcggcgagc acgcgatagt cctcgatacc agcctcgtag gtgcccggcg
     5101 cggggccgct cgcgctctga tataggccgt tgttagcggc tgcgactccg gtccagcccc
     5161 ggccataaaa gggcaggccg agggtgagct tcgaggccgg cacgcccccg tccaagtagc
     5221 gctgaatggc cgcatccgca gaaaggccgg attctcgagc cgggtcctta ttgctgagga
     5281 gtaggggagc gtgaaagttg gtcgggccgg tcgcatccca ggcgccatgc atgtcgtaag
     5341 acatcaagtt gatccaatcg agatgtcgat ggatctggcc tagctcgagc agtgccgttt
     5401 gcggtgagcc cgcgggggcc gcgatcgtga gcaggagttt agggtcaatg gcgctgagct
     5461 ggcgccggaa ctcctccagg agcaacgtga aattgcgagt gtcctcgtcg cggtaaacgc
     5521 tgccgacgtc ccccgcgtat gccgggtact cccagtcgat gtctacgccg tcgaacaccc
     5581 cggcggctgc agccgcccca ccagcagggt caccgtcgag catggggagg ttaccgcgaa
     5641 tatacaagtc gacgcaggat ttggcgacgc gagcgcgtga ctccggcgtc agcgcggcat
     5701 ccgaaaaacc tttggaccac gtccagccgc ccagtgagat caaaaccttg atgttcgggt
     5761 agagcgcctt gagtttcttt agttgattga agttgccttt tagattttgg tcccaggtgt
     5821 ccgctttgcc atcgacgctc tccgtcgcct gaaaggactt ctgatagtcg gcaaaggcgt
     5881 cacccactcc ggcgcgagtg accatgacgc actccccgtt cacgatgtta ccgaatgcgt
     5941 aattgattgc ggtcagggtc ttcgccgagc cgctgtcgac gatgttcttc acgcgatagt
     6001 tgcgctggta aatgccccat tgagcaaaat acccaattac ctgctgagcg cttggtcgcg
     6061 gctcgggagc acccccgcta ctcggtgatc cgccagacat cggccctgat ccgccagacg
     6121 tctgcccgcc catagcgctg ctggttccac cgctggaggc attgcccgcg ttcgtggatg
     6181 aaccgcccgc actcgtggac tgacctgctt ggcctccagg gcttcccccg ttggccgagt
     6241 tacctgaacc gccctttgac gaggctccgc cattccatga cgaggggagc cctccgctcg
     6301 ctgttccgcc aaagccgctc ggggcggagg tgtaacccga gctttgccct ccgactgcga
     6361 cggtgtgccc gccttgtgcc gaattcccgg ggccgccggc gctcgatacg tggccgccgg
     6421 atgcgggaga gacaggagaa tccagggtgt ccgatgctga actgcatgcc ccaaagagtg
     6481 cgacatagcc aaagaccaga tacaggcgac tttcggagag catcaagggt gtcccgcttt
     6541 ctcgacgttg tggttcgcgc ttgtaccgtg cttgccgcga cgtcgatccg tggccgtgct
     6601 ccgttgaatt attggggggg tacggtgact ctaggcgcgg ccaccggctg aacatcgaag
     6661 cccgcgctca gccccaagat gggaacgaac cggggggctt cgtagaccac accaacaccg
     6721 tcgatttcga accgcggacg gcccagcgcg accgtgccct cgatgctcaa tctcaggtgc
     6781 acccgcggac tgacctcgag ctgtcccatc agcgcaggcg cgaccgcgta ccagggcgca
     6841 ttcgccgctc gaggggcacg gattccaaag ctcgaggcat gaatccatcc gacttgcagg
     6901 gccaagccaa gcccaaaccg gaggcgcccc gagcgttgcc agagagcggg tgcaaactcg
     6961 cccgtgatgg cccacagctt tgcgccggca gcgctcgggg aagccacgga atcgagggtc
     7021 gtcatggact ccgacgaagg cgacttgaga tgagatatcg agtgtggaat ccagccgcgc
     7081 agcgtcgctc ggtaatccag ccatggatac cgtaggtgcg cccccaactg agcggcgtag
     7141 gcggcgcctc caagccaatt cggggaccaa gcggtgccgg cgtggaatcc aaccgcggct
     7201 ggaggtctcg cggtcgtaaa cgactgggcc cgtaaggtca cctgggcttt tgacggaatt
     7261 ggagtaaggc ttggagcgac tgccggcccg cggagctcgg acccatcccc aaacgacaat
     7321 gcgatactca gggaggtggc gcgaacgagc gcctcgcaat tctccgagcg gaactgacgc
     7381 tgggtcgatt caccatcccg gatgatctcg agcgaaacct cgtattgctg gggctcgtgt
     7441 ggccgatcta tgcgctgcac acgggcgtgg gccgtgactc tgggcaggtc tcgagttccg
     7501 cccggcgacc tcgggagatg cagcatctgc tgaatctcgg tccttacgcg cgcgctggct
     7561 gcgcactctg gcggtgcttc ccaaatcagg gtcagaggtg aggtggggcc ggcttgggtt
     7621 tctggcccgg gaggggcgct gctttgggcc caggcttggc ttgcgatcaa cgagattgaa
     7681 gcgccaacta gggctgcggg ctttgtcgaa cgcaagccca acgaatcgtg cggcttcgag
     7741 gaccttcggc aactcaccga tctatacagc tcccgcgcag agcggcggaa ccgctgatcc
     7801 cgctcgccgc gcgccgggct tcttccacac ggtgggaagc gcagagtgcc aagaccttca
     7861 gcgtgctccg ttccgcgctc agctgcgagc gcgggaatct cttcacgtgc tcgctcacga
     7921 gcgtcagagc atctgcggcc ctgccgctgc ggtaggcggc attggccttt tgcaagagcg
     7981 ccacctcggc ggctaagtcg tccttcggta cgacctcgct gcgtggcgcg ggggtgtttc
     8041 gggaactggt ggcgtttcgc ggcagagcgt cgggaagcgt tcgtgatatc gaatctcgcg
     8101 tctgtggcgc gggggcaact ggcgtttgct cgcggggaga tgtagggttt cccgaactag
     8161 ccagcactgg tgcatcgagg gtcgaagcct cgtcacggct cggcggtgac cctgccggcg
     8221 agcgatcctc gtgtggcgca gcggaactca cgacggctgt tgctgcggga ggagagacgg
     8281 cacgcgaaat ccaaacgccg cccagcgccg ctgagcccgc gccgagccca agcagggaaa
     8341 aggtcagcgc gtgtttggac aaccaaagct tgagagagct ctgcgtggct tgggccgcac
     8401 cacctgctgt cagcccgccg gcaatcaagc tggcctgcag cgttcgcaac attttcgcct
     8461 ttcggtcgga gctgggcgcg cgcgcctgat aggcagagcg aagctgcgcg gacacatcag
     8521 ggtccaattc cgtcatggtg agcctcctcc cggagccgcg cttccggcca cctctttgac
     8581 gatcagccgt gccgttcgga gccgggcgcg cagcgtattc tcattcactc cgagcatcgc
     8641 cgcgacttcg acctgaggta gttgttccaa gtcacacagc acgaaggcct ctcggggtag
     8701 ctccgccatc tgcgcgagcg ctcggtcgag cagttgcaaa gtacgcttca actcgagcgc
     8761 gtcgcacgcc gtgccctcgg gcgaccggaa agattccagc tcttcgattg gctcgcccag
     8821 ctgtcgattg cgccgccgcc gtcgaaaccc gcggaccacg ttcacggcga tgccagcgat
     8881 ccaggtcttg actccggagc ggcgctcgaa gctagcgagg cgcgcgtgca cgaccaggaa
     8941 cacctcttga acgacatcat cgagttgggc cggtgcgatt ccatgggtag ccgcgtaacg
     9001 ccaaacgaag tcgacatgct cttcatagag cgtctcgaag ctcggtgccg gctcgcttgc
     9061 tggacgcgct accatcgact ggggcccggt ctggccttcc gagactgcag accagagccg
     9121 cgagaaggtg gctgagctct ccaccctcgt caatgctctg ctttggccga ggtggtgccc
     9181 ggtcaagcgc cagctccgca ataacaggtt gccaaaggcg aatgcccagc cgtgaaatgc
     9241 gcacttccgc cgcgtccgtg gggaccacca tggccgccgg aggtcacgtt cgcccggcgg
     9301 gaatcgcgta tgctaagcgt cggatgtcgc ctggtgcttg cgcgtggtct cgggttcggt
     9361 ccacagtcgg acgcggcagg cctttccgcg tggcggctac cctcgtgggg ttcggtcacg
     9421 aaaggctccg cgattgcttg atttcgttgc tcatggcggt cgcgaccagc ggcaacatag
     9481 gctgcctgcg caatccctat tctctcggca gtgacgtcga gagccagggt gggggcggcg
     9541 gggcgcctca gagtacgacc ggcggcaacg ccccgggaat cgactgcgtt aacggcacgt
     9601 gtaccatcga ctgccaacgt ggcccctgcg ccactcccgt aacctgccct gatggacgcg
     9661 cttgccacgt gctctgtgga gacgaagcct gctctgcggg cattgcttgt gcgcccggca
     9721 ctccgtgcag cattgactgc cttgggcgtg gtgcgtgcgg gggaaccgta gattgcgtcg
     9781 ctgcgtcctc gtgctccgtt cggtgtaccg ggcttgccag ctgcaccggc ctcatctcga
     9841 gttcggcgtc gagtacccag gtggcttgca ccggcgcggg ctcctgtctc ggtgaggtcc
     9901 acacgcaagg gggaccggcc gatatctcct gcctcggcga gaacagctgt tccgctccgg
     9961 tcgactgcca gagcgaaagt tgtcgggtcg agtgcactca ggacgcgagc tgcaccagca
    10021 tatgctgccg ggcggctcaa tgtgagctct ccgagaacat cggtgggggg tgccggtaag
    10081 ctcatgcgtc agccctaccg tgcccgtccg aggccaatcc gtgggagcct ccgtacttga
    10141 gcctacaaaa aaccgaaaac tagccggcgt caccagctat ctggacgttt gcgccttcaa
    10201 cagcgcttcc aactcggcca ctcgagcgag ggcggcctcc ttggcttgcc gctctgcctc
    10261 cttggcttgc cgctctgcct ccttggctag tcgctctgcc tccttggcta gtcgctctgc
    10321 ctcctctccg gtggggaaaa ggtgttctcc gtccgggtga agagcgagcc tcaggcgttg
    10381 atgcatcccc gagcccacca gccgcaaata gcagcccagc gagcgggacg gcacccgatc
    10441 ctcgctcgtt gtttcacggc gaagcagacg gtcgcccact cgttcgtagc gttgaaagcg
    10501 gaggccatcg ctacgagcct cataacgcgg atcgaagatg atcagctctt gggttccgat
    10561 ctgttcgtgc aaggcagggg ccgcttcata gtctttgcgc caatcctggg agcagacctc
    10621 cagcgcaaag cttggaacga cgcgcgcttc ccaagtcttc caagactgca catccgtatc
    10681 cagcgccacg ccaggcagta cgtagatgtc gggagccacg cgttttgccg gctggcgtgc
    10741 atcgaagtaa ataaactggt cggcacccac gaagctgggc gagccgcgag tcacgagcca
    10801 ccgctcgatg agcggccgca gcaactccat gatccagcgc tgcagaatgt cctcccccaa
    10861 gcgctcctcc acggggaaat cttcttcccg gtaatccggc gggtgggcac tcggggttct
    10921 tcgcgcttgc atgccgaaca accatatcac gcgaccattg tg
//
LOCUS       DASTKU010000859         4824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_613485, whole genome shotgun sequence.
ACCESSION   DASTKU010000859 DASTKU010000000
VERSION     DASTKU010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613485"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VFQ61_30200"
     CDS             complement(<1..1003)
                     /locus_tag="VFQ61_30200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HET9958818.1"
                     /translation="MTARAPTRSVRGNPYFHGDNLDSVDTTSANDGTFTRQGFDAFGA
                     PTDPLAATRSGFTGHQHDEDLGLIDMRGRVYDPLAARFLTQDPIRSAPYWTQGQNPYS
                     YVFNDPINATDPSGFVSDSFWRNGADSGTAIEGWGVGVGAAGMGASFGGMVLGGGANV
                     ATSFGLNALMGDPFAGSASQTKAGAIGRNVAGVKGGGNGSAGTRQNQPDSAEAMGGKG
                     VRRPSLFDPEAGALACNAGGDVCIEILRQQRAAQEKIIQENIEAVRDAEWELFWAYTG
                     AQAAKLVRWGWVGFKAWRAARAASAAVKSGLRIVEQVDDVVEIAGEVGGHEVRVLANI
                     "
     gene            1227..2483
                     /locus_tag="VFQ61_30205"
     CDS             1227..2483
                     /locus_tag="VFQ61_30205"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET9958819.1"
                     /translation="MAEAKLFVGIDWGSETHQVCVINAQREKLLEYGIEHSGSALTGL
                     AEKLLQLAGGDPQRVAVAIEVPRGPVVEALLEREIPVFAINPKQLDRFRDRHTVAGAK
                     DDRRDALVLADSLRTDQSAFRRVQLGDANLVQLRELCRLQEELKAERNALGNRLREQL
                     HRYFPQILELGSVYDSPWIWALLTKAPTPQQAARLSVAKLGSLLKLHHIRRLTPEQVR
                     DILKSEPLHVAPGVLLACQRSVASLLPRLQLAHEHKHDVEREIGELLEQCSKPDPEGK
                     VEHRDALLLQSLPGLGKLVCATMLAEASEALARRDYTTLRGLCGVAPVTRRSGKQLAV
                     AMRHSCNHRLRSAVHYWATNAPNYDDYWKARYHALRAKGHQHARAVRGIADRLLAVLI
                     AILKSGKPYDPTHKRAVPAALVTPAS"
     gene            complement(2691..3200)
                     /locus_tag="VFQ61_30210"
     CDS             complement(2691..3200)
                     /locus_tag="VFQ61_30210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958820.1"
                     /translation="MDDLLARLRTLTKQERYDAAIALCDTALASTEGDGRARLWRLRA
                     YVHQQMGRDDLAIIDASTGLLEQPGELSLLFERATVYLELRQFAEANADLEQLLDIEQ
                     RQGGDFFDGAARIFQAICLSNLGRADDALPIILGLREDARTWALGRLWTRDELEAENR
                     AQLSTRQTF"
     gene            complement(3318..4031)
                     /locus_tag="VFQ61_30215"
     CDS             complement(3318..4031)
                     /locus_tag="VFQ61_30215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HET9958821.1"
                     /translation="MLAGTHESQYDYDGQSRRVRITEKENNVQTKQETFIWCGSRICQ
                     KRSGTTVLRSYFMNGFEENASSDYFYTRDHLGSVREVVASDVTTVASRLSYDPWGKLT
                     ETGTVLSDFTYTGHYYDRSSGLALAWLRGYDPNLGRWLSQDPIGLRGGWNLYGYVEND
                     PTRRADPTGEFGVALPVVVVAGAIVVTGVAIWAILEGNQKALDQLVAMSATFPDGSLF
                     QILTNVERSGTKLGKRAFK"
     gene            complement(4025..4237)
                     /locus_tag="VFQ61_30220"
     CDS             complement(4025..4237)
                     /locus_tag="VFQ61_30220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958822.1"
                     /translation="MDLAVGTNTVVVQSRDANDNVATKTYSVTTSGTSKNFEYDAYGN
                     LRYESSPTAPSSANTAGISKTASCAC"
     gene            complement(4376..>4824)
                     /locus_tag="VFQ61_30225"
     CDS             complement(4376..>4824)
                     /locus_tag="VFQ61_30225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958823.1"
                     /translation="GRASSLDMSCLCFGAGDSTSVEGQNINLPSAWERREQAGRAAHL
                     ALHRAAPPFPPSRTSCEVRVERAIQREVEAHLLEFDVRRVLGVTGFRYPFERDLDTMA
                     REQRAEWLREYFLSHPEGEHGVPGFVSQYRKRCKDTRVVAERVEED"
BASE COUNT          866 a         1564 c         1418 g          976 t
ORIGIN      
        1 atatgttggc gaggacgcga acctcatggc cacctacctc ccctgcgatc tcgaccacat
       61 cgtcgacctg ttcaacgatt cggagtccgc tctttacggc agcagaagcg gccctcgccg
      121 ccctccaagc cttgaacccc acccaacccc agcggacgag ctttgccgcc tgagcccccg
      181 tgtacgccca gaacagctcc cactcagcgt cgcgcacggc ctcgatgttt tcctggatga
      241 tcttttcttg ggccgcgcgc tgttgtcgga gaatttctat gcagacgtct cctcccgcgt
      301 tgcacgccag cgcccccgcc tccgggtcaa acaagctcgg ccgtcgaacc cctttcccac
      361 ccatcgcctc cgcgctgtcc ggctggttct gccgcgtccc ggcacttcca ttccccccac
      421 ccttcacgcc cgcgacattc ctcccaatcg ctcccgcctt cgtctgcgac gcgctccccg
      481 cgaaggggtc tcccatcagc gcgttcagcc cgaacgacgt tgctacgttc gcgcccccgc
      541 ccagcaccat tcccccgaag ctcgctccca tgccggccgc accgactccc acgccccacc
      601 cttcaattgc cgtcccgctg tccgctccgt tccgccagaa cgagtccgac acgaacccgc
      661 tcggatccgt cgcattgatc ggatcgttga acacgtagct gtacgggttc tgcccctgcg
      721 tccagtaagg cgctgacctg atcggatcct gagtgaggaa gcgcgcggcg agcggatcgt
      781 agacgcgccc gcgcatgtcg atgaggccga ggtcttcgtc gtgttggtgg ccggtgaagc
      841 cgcttcgcgt cgccgcgagc ggatccgtcg gggcgccgaa ggcgtcgaag ccttgtcgcg
      901 tgaaggtgcc gtcgtttgcg ctcgtcgtgt cgacgctgtc gaggttgtcg ccgtggaagt
      961 aggggttgcc ccgcacactg cgcgtcggtg cgcgcgctgt cactcgtgcg cttgcagcgc
     1021 acggtctccc ctgtccgctc tgcgagtctt ggccgcctca cgcggaacac cagcggtctc
     1081 ctgcctaacg aaggccgttt ggggacttgt gtcccgtgag gtgggccgtg acgtacatcc
     1141 gatgggaagt tgcgtcatcg ctttgatgag cgccggtaca gggttgatga gacgtcatgg
     1201 cgggccaggg cagaaggagg tgcgacatgg cagaagccaa actatttgtc ggaatcgact
     1261 ggggctcgga gacgcaccag gtttgcgtca tcaacgccca gcgcgagaaa ctactcgagt
     1321 acggcatcga gcattctgga agtgcgctca ccgggctcgc tgagaagctc ctgcaactgg
     1381 ccggcggtga ccctcaacgc gttgccgtgg cgattgaagt gccgcgcggg cccgtcgtcg
     1441 aggcgcttct ggaaagagag atcccggtat ttgccatcaa tccgaagcag ctcgaccgct
     1501 ttcgagatcg acacaccgta gccggcgcca aggacgaccg tcgcgatgct ttggtgctcg
     1561 cggattcact ccgcacagac caatccgcgt ttcgccgagt gcagctcggc gacgctaacc
     1621 tagtgcagct tcgagaattg tgccgcttgc aggaagagct gaaggccgaa cgaaatgctt
     1681 tgggcaaccg cctgcgtgag cagctgcatc gctactttcc acaaattctc gagctcggca
     1741 gcgtctacga ctcgccgtgg atttgggcct tgttgacgaa ggcgcccacg cctcagcaag
     1801 cagcgcgcct cagcgtcgcg aagctcggct ccctgctgaa gctgcaccac attcggcgac
     1861 tgacgcccga gcaagtccga gacatcttga agtctgaacc gctccacgtc gcgcccggcg
     1921 tcctgctggc gtgccagcga agcgtcgcct cattgctgcc gcgacttcag ctagctcacg
     1981 agcacaagca cgatgtcgaa cgcgagattg gcgagctgct cgaacagtgc tcgaagccgg
     2041 accccgaggg aaaagttgag caccgtgacg cactactcct ccaatccttg ccgggcctgg
     2101 ggaagctggt gtgcgccacg atgcttgccg aggcctccga ggccctggcc cgacgagact
     2161 acaccactct acgtggcctt tgtggcgtcg caccggtaac caggcggagc ggaaagcagc
     2221 tcgcagtcgc catgcgccac agctgcaacc accggcttcg ctccgccgtg cactattggg
     2281 ccaccaacgc gccaaactat gatgactatt ggaaagcccg ctaccacgcc ctacgagcca
     2341 aaggccacca gcacgctcga gcggtcagag gcatcgccga ccgattgctc gccgtgctca
     2401 tcgcgattct caagagcgga aaaccgtacg accctaccca taaacgcgcc gtacccgccg
     2461 cactcgtgac gcctgcctcc tgaatatttt tctcttgccc acgactggga agtccccttg
     2521 cttgtgctcg cgcgcgaacg gcaccgtcaa ggctgcgcgc gctgcgcgca agcggaggag
     2581 acacgctctg cgcgccttcg ctgacttgcg ggggttgtcg tctcaagcgt gaccaatgca
     2641 gaacggaatt gagtattcag cgtcattggt gccgcgttgc gcgatgcgcg tcaaaacgtc
     2701 tggcgcgttg atagttgagc acggttctct gcctccagtt catcccgagt ccacagccgt
     2761 ccgagcgccc aagttctcgc gtcttcacga agacctagga tgatagggag cgcgtcgtcg
     2821 gcgcgcccca aattgctcaa acagatcgct tgaaagattc gcgcagcccc gtcaaagaag
     2881 tcgcctccct gtcgttgctc gatgtctaag agttgctcaa gatcagcgtt ggcctctgca
     2941 aactgacgta gctccaagta gaccgttgcg cgctcaaaca gcaacgagag ttcccctggt
     3001 tgctccagca gaccggtact ggcgtctatg atcgcgaggt cgtctcgacc catctgttga
     3061 tggacgtaag cgcgcaggcg ccacagccgc gctcgcccat caccttcagt cgaggcaagg
     3121 gcggtgtcgc acaatgcgat ggccgcatcg tatcgctcct gttttgtaag cgttcgaagt
     3181 cttgccagta gatcgtccat gctccaaagc cattcagtca agcggtaggt cagggcgtgg
     3241 atttcccgtc ggtcaccctc cgcacttttc gctcatgtag tcacgcatgc agtcttccaa
     3301 tgtgcgccct tttcgactca tttaaaggca cgtttgccga gcttcgtccc gctcctttct
     3361 acattcgtca gtatctggaa aagggagcca tctgggaaag tcgccgacat tgcaaccagc
     3421 tgatcgagtg ctttttgatt gccttccaga atggcccaga tcgcgactcc ggtgaccacg
     3481 atcgctcccg ccacgacaac aacgggcaat gcgaccccaa actcccctgt cggatccgct
     3541 cgacgggtcg gatcgttctc cacatacccg taaaggttcc acccacccct gagcccgatc
     3601 ggatcctggc tcaaccatcg cccgagattc gggtcgtacc cacgaagcca cgcgagcgcg
     3661 agtcccgagg acctgtcgta gtagtggccg gtgtacgtga agtcgctcag cacggttccc
     3721 gtctccgtca gctttcccca agggtcgtag gagaggcgtg acgccaccgt cgtaacatcg
     3781 ctcgcgacga cttcgcggac gctgccgagg tggtcgcgtg tgtagaagta gtcgctcgag
     3841 gcgttttctt cgaagccgtt catgaagtag ctgcggagca cggtcgtgcc gcttcgcttt
     3901 tggcagatgc gcgagccgca ccagatgaag gtttcctgct tggtttggac gttgttttct
     3961 ttttcggtga tgcgcacgcg gcgggactgg ccgtcgtagt cgtactggga ttcgtgagtg
     4021 ccggctagca tgcgcacgag gcggttttgc tgatcccagc ggtattcgcg gatgacggtg
     4081 ccgttgggct gctttcgtag cgcaggttgc cgtatgcatc gtactcgaag tttttcgagg
     4141 tgccgctggt tgttaccgag taggttttgg tcgcgacgtt gtcgttggcg tcgcgggatt
     4201 gaaccacgac ggtgttcgtg ccgacggcga ggtccacggg ggcctcgaac ttgaacggag
     4261 ccgtgcccgc tgtggaggtt agtttcgcgg ggttgccgtc gaaactcgtg acgcaagcgg
     4321 ataactctca ctcgtgtaga tgacgtgcac ccggcgacat tcgccaccaa tcgcctcaat
     4381 cctcctcgac tcgctcggcg acaacgcgag tgtctttaca gcgttttcga tactgcgaaa
     4441 cgaagcctgg aacgccgtgc tccccttcag gatgcgaaag gaagtattca cgcagccact
     4501 cagcgcgttg ttcgcgcgcc attgtgtcga ggtcgcgctc gaacgggtaa cgaaagccgg
     4561 ttacgccgag cactcttcgc acgtcgaact cgagaaggtg cgcttcgact tcgcgttgaa
     4621 tggcgcgctc gactcgaact tcgcaagagg tgcggctggg cgggaaaggt ggtgcggcac
     4681 gatgcagcgc gaggtgggca gcgcgaccgg cttgttcgcg acgctcccac gctgaaggta
     4741 agttgatgtt ctgcccctcg acgctggtcg agtcgcccgc gccgaaacag aggcaactca
     4801 tgtccaaact tgaggctcta ccga
//
LOCUS       DASTKU010000860        19451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1731025, whole genome shotgun sequence.
ACCESSION   DASTKU010000860 DASTKU010000000
VERSION     DASTKU010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 19451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19451
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731025"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VFQ61_30230"
     CDS             complement(<1..916)
                     /locus_tag="VFQ61_30230"
                     /inference="COORDINATES: protein motif:HMM:NF012236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscN/YscN/HrcN family type III secretion system
                     ATPase"
                     /protein_id="HET9958824.1"
                     /translation="MLDRLIERCRTAPVHKPEGVVLDVVGLIVEIGGLRAGVGDTLAL
                     KDAYGGSLEVEVIGFRAGRLLTTPLGSLSGVRPGARVVETQRGGSVPASNQLLGRIVD
                     PFGEPLDGGPRIVADRVQPVHAPPPAPFARRPIEKPFRTGVRAIDGLLPLGVGQRMGI
                     FAGAGVGKSTLLGMICRSSQADVNVVGLVGERGRELNDFVRNSLGAEGLAKSVVVTAT
                     SDQPPLVRARGAEAATAIAEYFRAQGLSVLLVMDSVTRFAMALREAALTAGEPPATKG
                     YPPSVFAALPRLLERAGTSGGAGVITALY"
     gene            complement(923..1738)
                     /locus_tag="VFQ61_30235"
     CDS             complement(923..1738)
                     /locus_tag="VFQ61_30235"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HET9958825.1"
                     /translation="MRWFDRQASEGTKRVKFAEVAPAPAPRRAPSWMAPRPEPERRTE
                     SSLKPPKLPSEFVDAVRHEIGEEFVARTRGSLIPEATGPRSEVPPSSTAFNPVPAAQP
                     SFPPPAATKSLVPAAKAMPIVERVPVVDPEIGQAFERAIAVLAIERERVLSETAGQLA
                     ELAVLIARRVIGRELGLHPDIVRGLVREGIAALGHHDRVLVRVGRGFAAGREALERDL
                     SDTDKRFEVRVDPALEVYGCVVETELGQVDESIESRLETLLSALRPESNGPGV"
     gene            complement(1725..2711)
                     /gene="fliG"
                     /locus_tag="VFQ61_30240"
     CDS             complement(1725..2711)
                     /gene="fliG"
                     /locus_tag="VFQ61_30240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HET9958826.1"
                     /translation="MSIPNLTGAQKAVLMLLSLDEAAATPILAELDADEVKRLREAAA
                     GLTAVPARALESVYQEFIDRSQAAVAVPGGGVKYLRKLTGRALGEERAQQIFHEVQET
                     PMDRLAAADPIALGSILEHEHPQLVAAVLSQLDNERAARVLEQLPVELRPLVLERLGS
                     MREVPAALLEEVATAISAELPNTGGEAVRNIDGIARSAALVRRMDRQVGEALLGTIEE
                     GNSELAVEIRRAMYTFEDLGALDARALRSLLEAVPVEKLTVALKTASESLKNHVFSSM
                     SKRAAERIREDMESTGGVRLADVEAAQREIVEVALRLDAEGTISLENNNAMV"
     gene            complement(2776..4434)
                     /gene="fliF"
                     /locus_tag="VFQ61_30245"
     CDS             complement(2776..4434)
                     /gene="fliF"
                     /locus_tag="VFQ61_30245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HET9958827.1"
                     /translation="MPERLRTVLQNLSQFWAKLSVGKRVALIGVTGAVLLLVSAITVL
                     GNREHYTYLYTELSTEDAAAIVEKLKTQQIPYKIEASGTALMVPEEKVAALRLELASS
                     GLPRGGRVGFEIFDQSRIGATEFEQQVSLRRALEGELSRSVMAIHGVKSARVHLVMPE
                     RRLFTARSEAASASVVIGLAPSAAFGRREIATIVHLVSAAVPGLSKDRVSVVSTDGAT
                     LHRPVSDGEDASDLTDIQVTQREQARTMATRYESDVLAQVERVVGSGNADVRVNVELD
                     TSTRERTEERYEPSKTALRSENQQEEVSAAQEAGVAGVPGARTNLPDAAAEGETPAAP
                     TAAAGGGTVRRSHTRNWEVERVTQKTTIPAGDMRRVSVAVLLNGRWEMRGNKPSFVPR
                     KPEEVAVLESVVKNAVGFDPARGDTVVVQAAQFARNEEPTPEAEPRWVVASRRYGPLA
                     LAGAVLLIVLVAALVMRSKRAAARRQAELQARAQQAALASGNAAGVQALPEGVSAAEL
                     PAHQAPSFEEIRQQALELAAKDPARAAVVLREWLGSPAVPALSQ"
     gene            complement(4620..4943)
                     /gene="fliE"
                     /locus_tag="VFQ61_30250"
     CDS             complement(4620..4943)
                     /gene="fliE"
                     /locus_tag="VFQ61_30250"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HET9958828.1"
                     /translation="MPIEGPTFPLFAPHAAFEATPTKAEQAEATDAAASGVFEDLLKS
                     MAVSANQSIQQAAAQGEAFAAGTRDDIHGTMVSLSKADIELRVLGNVRNRVIDAFYEI
                     WRMQI"
     gene            complement(4997..5422)
                     /gene="flgC"
                     /locus_tag="VFQ61_30255"
     CDS             complement(4997..5422)
                     /gene="flgC"
                     /locus_tag="VFQ61_30255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HET9958829.1"
                     /translation="MLGVFSALEISASGLSAERVRMNTISSNLANARTTRTEEGGPYR
                     RIDPVFEAVALERGLNVQDPGAATVRVSRLVEDQRPGTMVYEPGHPDANPAGYVEYPN
                     VNAVEEMVNMITASRAYEAGITSIETVKQMARSALELGK"
     gene            complement(5490..5894)
                     /gene="flgB"
                     /locus_tag="VFQ61_30260"
     CDS             complement(5490..5894)
                     /gene="flgB"
                     /locus_tag="VFQ61_30260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HET9958830.1"
                     /translation="MSDVFSGIGPLQAALDYHLERHSVLSSNVAHVDTPGYRPADLAR
                     IDTEPFAAVLDVSMNRTNSAHLQGPAPNQGLSHGKLFRDVSAGAGNDENFVSLDREAA
                     KVAANQLRYDTVSAIVSAELRQLHYAASDGKG"
     gene            complement(5887..6045)
                     /locus_tag="VFQ61_30265"
     CDS             complement(5887..6045)
                     /locus_tag="VFQ61_30265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958831.1"
                     /translation="MHTAAALPSLFASPVLRTEPRLLGLTNSGLARQVLNSEAMPPAI
                     AEQLFAHV"
     gene            6430..7602
                     /locus_tag="VFQ61_30270"
     CDS             6430..7602
                     /locus_tag="VFQ61_30270"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9958832.1"
                     /translation="MAKGYAVDTMDDLSIEDSSSGLRDLSAIALCSSGPEAMEDEESI
                     ADEQGEEQVEEPVTNSARIRESKLPQSMQGVVGIAESLVEVYRIVDRVADTTCNILIT
                     GESGTGKEVVARAVHRVSPRAQAPFVAVNCGAIPEALLESELFGHAKGAFTGAHQSKP
                     GRVALAQGGTLFLDEIGEMPLSLQVKLLRILQSREYSPVGDNRVLKADVRVVAATNVN
                     LENAVQAGTFREDLYYRLNVIHLTVPSLRERPEDVPLLVQHFLLKSREKLARGAISIS
                     RAAAQLLAEYHWPGNVRELENTIERAVLLCAGGVIEPKDLPQRVLGLGTEKRIAARLP
                     DSGLDLRNAVETFENQLIRQALERTKWNKKQAATLLGLNRTTLVEMLKRKRITRAA"
     gene            7634..8191
                     /locus_tag="VFQ61_30275"
     CDS             7634..8191
                     /locus_tag="VFQ61_30275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958833.1"
                     /translation="MTRSPALEGVRRVHGARRSSAFGAASALLLALLWTARARADGYA
                     VDGTLGVGSGMQAGDAGSGMAWSRARLRLQTGFDLMSDELPNQAVGFRGFVELERRGS
                     VGLEARYTRWLGRGFGGFVFGSAAFMPETLVGGGVGFTLVIPLGSRVGLGLEPAFAAF
                     PLGSDLPEGSFVFLAGLSAGVRVRL"
     gene            8188..8517
                     /locus_tag="VFQ61_30280"
     CDS             8188..8517
                     /locus_tag="VFQ61_30280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958834.1"
                     /translation="MKVSPAIWLSLLLGGHAASCAQPGQCFRHTDCARGEHCELGACV
                     KDSTGNSSPGDAGAGGEFSGSGAAPSADGGKSSGGTQASSSKPGSSGAGGAGGAGGEA
                     ADSGAGG"
     gene            8561..9346
                     /gene="flgG"
                     /locus_tag="VFQ61_30285"
     CDS             8561..9346
                     /gene="flgG"
                     /locus_tag="VFQ61_30285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HET9958835.1"
                     /translation="MFRSLHIAATGMAAQETHLESISNNIANGNTVGFKKQRVDFQDL
                     LYQTVRQPGAPTGATTTSPTGLQLGSGVRVVSTARIYEQGTVLTTKNPLDVAIEGDGF
                     FVVQQNDGKPGYTRAGALRTDPQGRIVTPEGMPLDPPISVPQGATNVSISANGRVSVT
                     LPGEKDPAEIGQIQIATFINPSGLQAQGHNVLTASAASGDAQVGEPGTEGRGSLLQGA
                     VEQSNVDIVEEMIGLISAQRSYEINSKVITTADDMLRAATQIR"
     gene            9343..10125
                     /locus_tag="VFQ61_30290"
     CDS             9343..10125
                     /locus_tag="VFQ61_30290"
                     /inference="COORDINATES: protein motif:HMM:NF024544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HET9958836.1"
                     /translation="MIHLSYPEHRRGRRAALLGVVLGLVTLSSSAVGEPIRVVDHPRV
                     RLRDLSTALAALPAPLPEVDLGPSPPPGGSRLFARESVLAELRTQGLEPKGVNLPPVV
                     RVQRAARHIEPSELKALLEPQLLAALGPNMKLASFDIKKGLLTVPNPEVGPVRLPQFS
                     RREGPIQLTVVADLLHDGNVVARLPVPIVIELSAAALQPSVAKGARVDLVIERGNARI
                     SAAATALEAAELGQVIAFKVSTTSRVLRARITSPTSALVVSP"
     gene            10122..10814
                     /locus_tag="VFQ61_30295"
     CDS             10122..10814
                     /locus_tag="VFQ61_30295"
                     /inference="COORDINATES: protein motif:HMM:NF014195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HET9958837.1"
                     /translation="MNSSRFLLLALGLMACGPAHIAPFTPKQRKYEPGAYAATQKGSQ
                     PATGSIYSEAQPGFLEDTRALRVGDTVLVRINEHADAQGNATTNLNKESNREVGAEAM
                     LGLVPAIRKSYPDIDPSKLLSLASSFDFKGQGSTQRAGTLTAAIGVHVKQELPNGDLF
                     VEGTKVVMINHEEYHLYISGVLRPADIEQDNSVDSTLIADARVEFTGRGDIDDQVNRG
                     WLTKLLDFVNPF"
     gene            10827..11957
                     /locus_tag="VFQ61_30300"
     CDS             10827..11957
                     /locus_tag="VFQ61_30300"
                     /inference="COORDINATES: protein motif:HMM:NF003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HET9958838.1"
                     /translation="MSPSRLLPALRLLAFWCVAFGVLLSTRAARADHLRDLCDVVGVR
                     ENQLVGYGVVTGLNGTGDDLSAPFATQSLRALLRRIGVQVDSKQIRLKNIAAVLVTAN
                     IPAFARPGSKLDVTISSIGNSKSLRGGVLVQTPLRGADRKTYAVAQGALLLGGFSAGG
                     SGASVQDGVTTTARLPNGALVEREIPTTFANQGKVTLALKSPDFSTAQRIVAALEKEL
                     GEGNARALDGGAIEVKAPKELASKPVELIARLSEVEVDPVTAARVVINERTGTVVAGG
                     DVRLSPVAIAQGGITISVQETRTISQPGPLSGGQTREQQNTQVTAEEGRPAQTLSYLK
                     GATTLAEVAQALSTFGISPRELASILQALKTAGALRAEIVVQ"
     gene            11971..12432
                     /locus_tag="VFQ61_30305"
     CDS             11971..12432
                     /locus_tag="VFQ61_30305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958839.1"
                     /translation="MIGPLNALRAESAAATTSTSSGTNAATPRPEVLRAARDFEAIFL
                     RTLLKPLENTTRLSTGGQSLPGQSAYGSMVVGALSDSLAASGGLGLAGVIARAMTASD
                     PASALPPSAGQPGSPSSTSAEPSPDVTRSPRLASPGAPTNIEASSARSGLK"
     gene            12575..12862
                     /gene="flgM"
                     /locus_tag="VFQ61_30310"
     CDS             12575..12862
                     /gene="flgM"
                     /locus_tag="VFQ61_30310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HET9958840.1"
                     /translation="MKGITGNPALDAYHRMGISPVGNARPAERVQGGSQAAVSSPAAQ
                     VTISARARDLASESAGSVDPSKVEELRDRIASGNFQVDPRAVANKMMEQLG"
     gene            12908..13273
                     /locus_tag="VFQ61_30315"
     CDS             12908..13273
                     /locus_tag="VFQ61_30315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958841.1"
                     /translation="MSEPLLDDDPASTLSALEALHEEELSVLRNMDRASLDDLTARKE
                     QLCEQLRAAQLSGKLTDRHRSQIERIRQKAAHNQLLVVHARDAVRGILSQATGVCLDV
                     LPSGRRGSTQEGLRLNVRG"
     gene            13273..14661
                     /gene="flgK"
                     /locus_tag="VFQ61_30320"
     CDS             13273..14661
                     /gene="flgK"
                     /locus_tag="VFQ61_30320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HET9958842.1"
                     /translation="MSTLSQLLSTARDGLTAQSYGLGVTGQNISNVNTPGYVRREAML
                     ETRALGTQTTGSVEAVGLRRVTDTFLERRQFEATGLSSAASEHDSRLASLEALFNDSA
                     GTGLGGAVDAMFGSFSRLAASPSDSTVRQSVLDSASNLAQRVRDTANTIAKGRQDLLQ
                     DAQATTTQVNDRSRAIARLNTQISAAETQGNDAADLKDQRNRLLLELASMVDVRTIVS
                     GSGIMVQASGTTLVEGGEARELSISVDSSSRMQILASRSDGPPTDVTRFLTGGKLAGI
                     REARDVDLLEVGQKLDQFVFDVATAVNDQHRQGFGLDGATGRDIFAVTTVPEGAARAL
                     TLSADVAGNPNALAASSSAGALPGNSDNAAALSDLASTPVVFGAKRTAAEGYGDIVGD
                     VATRRADSRRMVESREAISAQIDAMHEAMSGVSLDEEFVNLTKFQRAYQASARVLTTT
                     DEILQELVNLKR"
     gene            14694..15572
                     /gene="flgL"
                     /locus_tag="VFQ61_30325"
     CDS             14694..15572
                     /gene="flgL"
                     /locus_tag="VFQ61_30325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HET9958843.1"
                     /translation="MRITDGLRYQSVQRNLTRIQKDAAEASQQASTGQRLGQPSDDPI
                     AAAELSRVRASLNTAAAQRDAIAMVRGDAELAESTLSQVGELMKRLRELAMQGANGSL
                     SAEDRDALATETRGIKTEVLRLANTRGTRGYLFAGARTDSPAFDAAGAFQGDDSTQLV
                     DIGATTPTAVSASGAQAFTAAGGRDVFADLDALANALDANDPAATAQSLDALDASERQ
                     VLKERARIGLVVDKLDTSNGVLEQLDTDLQKRGDALGTADAFEAYSRMTVLSQSLERA
                     VAVSRQMLESASYWNR"
     gene            15695..16576
                     /locus_tag="VFQ61_30330"
     CDS             15695..16576
                     /locus_tag="VFQ61_30330"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9958844.1"
                     /translation="MSSPAYPDPRPERASQAPPSSAPRSAGFSANLEGASLADLVQMA
                     CQSGLQSVARIRSAEQVGYLYFSQGRLVHAVSPNSVGDEAALEILSWQAGRFEPCGIE
                     PSAHVTIQSSYQSLLMRAAHARDERARGEPGRTDSAPRKVITLPPPRPRETAASMPEV
                     RDGSPREPSKTMPSLRDDERNEEARAESRLSSAERPRGGPRNTAAKATVRLDAEGNLR
                     FAQGSGTEELAQVAALVTRLGNLVGRALGLDRLHAVEGTTASGRTLIVIEKQGEVIGV
                     RTGPEDDVSTFRKTYGL"
     gene            16611..17210
                     /locus_tag="VFQ61_30335"
     CDS             16611..17210
                     /locus_tag="VFQ61_30335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958845.1"
                     /translation="MTAPALSPAVLHGLRDVEGVLGSFILSETGELLLRDLPGIFDNE
                     LFYEAGPRLTRLCDALSAQGEPLEAAGLRYAEHKLHLRRAQGCILCVLSTAQANPPAL
                     KMALSLLARRLEIRAAELRAPEMTASPQSPLPSGAGKPASAPPSGAQKSNGQGIVPGT
                     GHSVHGWGPSSGQSGARQSEPPPNGTARVIRTYRGRPIE"
     gene            complement(17232..>19451)
                     /locus_tag="VFQ61_30340"
     CDS             complement(17232..>19451)
                     /locus_tag="VFQ61_30340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958846.1"
                     /translation="GHRDICNGDDGATGREPAQAPAGQPGEDGRDGMLMLVAVSEEAP
                     GKNCEHGGQLVEIGPDLDQDGSIDTEVARESHYVCDGVAGQSGDSGVGSLVVSGEEGP
                     GDKCAYGGQRVVYGVDANTNGILDEGEVAQVRYVCRGAPGVTPAVPLFTVTPVAAGEL
                     CGAGGERIESGLDLDHNNALDPAEITTTRYVCTRSPGAPARVSLVNVATEPAGVRCAG
                     GGRRVDVGLDQNADHILQDAEILNTQYVCAGAPGAQGTAGQSALVPTVIKSPEAAGTH
                     CANGGQRIEIGFDADASGGLTGSEITQTEYVCDGGSGDPGEPGRLAVLAVVKTGSELP
                     GENCPAGGQSIQSGLDSNSNGMLDAGEVTRTDFVCYGSSALDGSNGVNALLGLTSVTE
                     EPAGTNCTAGGQAVSFGLDDDEDGTLDAEEIEGTRYICNGVAGTNGTVGAAGTDARRT
                     LVTVAAEPASPNCLFGGNRISYGLDDNSDSVLAPGEIDATRYVCNGAPGTNGTNGTSA
                     GNAVNSLVKLSAEASGVNCTGGGRRIDAGLDSNASGVLDATEIQSTSYVCDYDPFING
                     SFELANFQGWTVTGGIWGLVTEGTTLNQGSSILNYVTQQNSQLTSEALPYTADATDGT
                     RVAVNYQTGVANSHLIQDFNVPSGVTSLKWDMRYINTFGTFNATRQYIAVYVRSPKTN
                     TVLQQLYKTTSEAASQSAMITKTVDISAFRGMQIRLDFEIQVRDNWLDFAIDNVRMQ"
BASE COUNT         3443 a         6528 c         6146 g         3334 t
ORIGIN      
        1 tgtagagcgc agtgatcacg cccgccccgc ccgaggtacc cgcgcgctcg agcagacgcg
       61 gcagcgccgc gaacacactc ggcgggtagc ccttggttgc gggcggctca ccggcagtga
      121 gcgccgcttc gcgcagcgcc atcgcgaagc gcgtgaccga gtccatgacc agcagcacgc
      181 tcaagccctg cgcgcggaag tactcggcaa tggcggtggc ggcctcggcc ccgcgcgcgc
      241 gcaccaacgg gggttgatca ctggttgcgg tgaccaccac tgatttggcg agcccctccg
      301 cgccgagcga gttgcgcacg aagtcgttca gctcgcggcc tcgttcgccg accaagccga
      361 cgacgttcac gtccgcctga gaagagcggc aaatcatgcc gagcagcgtg ctcttgccca
      421 cgccggcgcc cgcgaagatg cccatgcgct gccccacccc gagcggcagc aagccgtcaa
      481 tcgcgcgcac gcccgtgcgg aagggcttct cgatcggacg acgcgcgaac ggtgctggcg
      541 gcggcgcatg cacgggttgc acgcgatcgg ccacgatgcg gggtccaccg tcgagcggct
      601 caccgaatgg atcgacgatg cgcccgagca gctgattcga ggccggaacg gagccgcccc
      661 gctgcgtctc cacgacccgg gcgccggggc gtactcccga aagcgatccg agtggcgtcg
      721 tgagcaagcg ccccgcgcga aagccgatca cctcgacctc gagacttccg ccgtaggcgt
      781 ctttcaaagc cagcgtgtcc ccaactccgg cgcgcagccc cccgatctca acgatcagtc
      841 cgacgacgtc gagcaccaca ccttcgggtt tatgaacggg cgcggtccgg cagcgttcaa
      901 tcagtcgatc caacatgggg cgctagactc ctggtccgtt cgactcgggc cgcagcgcgg
      961 agagcagcgt ttcgaggcga gactcgatcg actcgtcgac ctgcccgagc tcggtttcga
     1021 cgacgcaacc gtagacctcg agcgccggat ccacgcgcac ctcgaagcgc ttgtccgtgt
     1081 cactgagatc ccgttccagc gcctcacgcc cagcggcgaa gcctcggccc acgcgcacca
     1141 acacgcggtc atggtgtccc aaagcggcga tgccttcgcg caccagaccg cgcacgatgt
     1201 ccgggtgcaa gccgagctcg cgcccgatca cgcggcgcgc gatcaataca gcgagttcgg
     1261 cgagctgacc cgccgtctcg gaaagcaccc gctcgcgctc gatcgcgagc acggcgatgg
     1321 cgcgttcgaa ggcctgtccg atctccggat cgacgaccgg cactcgttcc acgatcggca
     1381 tggctttggc ggcgggcacg agtgacttcg tcgctgcagg cggggggaac gagggctgag
     1441 ccgcggggac cgggttgaat gcggtcgagc tcggcggcac ctccgaacgt gggcccgtcg
     1501 cctcgggtat caaactgccg cgcgtacgcg ctacgaattc ttccccgatc tcgtggcgga
     1561 cggcatcgac gaactcgctc ggcagcttgg gcggcttgag cgagctctcg gtacgtcgct
     1621 caggctccgg acgcggtgcc atccaagacg gtgcccgtcg cggggcgggg gccggcgcaa
     1681 cttcggcgaa cttgactcgc ttggtgccct ccgaggcctg acgatcaaac catcgcattg
     1741 ttgttctcga gcgagatcgt gccctccgcg tcgagacgca gagccacttc gacgatctcg
     1801 cgttgggccg cctcgacgtc cgcgaggcgt acgcctcccg tcgactccat gtcctcgcgg
     1861 atgcgctcgg ccgcgcgctt ggacatgctg ctgaagacgt ggttcttcag tgactccgag
     1921 gcggtcttca gcgccaccgt gagtttctcc acgggcaccg cctcgagcag cgagcgcaag
     1981 gctcgtgcgt ccagcgcccc gagatcctcg aaggtgtaca tcgcgcgccg gatttcgacc
     2041 gcgagctcgg agttgccctc ctcgatcgta ccgagcaggg cctcgccaac ctgacggtcc
     2101 atgcggcgca ccagcgccgc cgagcgcgcg ataccgtcga tgttgcgcac ggcttcgccg
     2161 cccgtgttcg ggagctctgc gctgatcgcg gtcgccactt cttccagcaa cgctgccggc
     2221 acctcgcgca tggagcccaa gcgctcgagc accaacgggc ggagctccac cggcaactgt
     2281 tcgagcacgc gcgccgcgcg ctcgttgtcg agttgagaga gtacggccgc cacgagctgc
     2341 ggatgctcgt gctccaggat cgaaccgagc gcgatgggat cggccgccgc caagcgatcc
     2401 atcggagtct cttgcacttc gtggaagatt tgctgcgcgc gctcctcacc gagcgcacga
     2461 ccggtgagct tgcgcaggta tttcacgccg ccgcccggca ccgccaccgc ggcctgcgat
     2521 cgatcgatga actcttggta cacgctctcg agcgcgcgcg cgggtacggc cgtgagaccc
     2581 gccgcggcct ctcgcaggcg cttgacctcg tccgcgtcta gctcggccaa gatcggcgta
     2641 gccgccgctt catcgagcga gagcagcatc agcaccgcct tttgagctcc ggtcaagttt
     2701 gggatgctca cgcttcagcc ctccgcgctc gcggcgccga ggaacgaaga gcttcgggga
     2761 agaatgctcg agagttcact gcgaaagagc cggcacggcg ggtgatccca gccactcccg
     2821 gagaaccacg gccgcgcggg ctggatcttt agcagccaat tccagcgctt gttggcggat
     2881 ctcttcgaag ctcggcgctt ggtgcgccgg aagctccgcc gcacttacac cttcgggtag
     2941 cgcctgcaca ccggcagcgt tgccgctcgc gagagccgcc tgttgcgctc gcgcctggag
     3001 ctcggcttgg cggcgcgcgg ccgcacgctt gctgcgcatg acgagggccg ccacgagcac
     3061 gatcagcaga acggcacccg ccagggccaa gggaccgtag cgtcgactcg ccacgaccca
     3121 gcgcggttcg gcttcgggcg tgggctcctc gttacgggcg aattgggcgg cttgaacgac
     3181 gacggtatcg ccccgagcgg gatcgaaccc tacggcgttc ttgacgaccg actcgagtac
     3241 ggcaacctcc tcgggcttgc ggggcacgaa agagggcttg ttgccgcgca tctcccagcg
     3301 gccgttgagg agtacggcca cgctcacccg ccgcatgtca ccggcaggaa tggtggtctt
     3361 ctgagtgacg cgctcgacct cccagttgcg cgtgtggctg cgacggacgg tgcccccacc
     3421 ggcggcagcc gtgggcgcgg ccggggtctc accctccgcc gcggcgtcag gcaggttggt
     3481 gcgcgcaccc ggcacgcccg ctaccccggc ctcttgagcc gcgctgacct cctcctgctg
     3541 attctcgctg cgcagggcgg tcttgcttgg ttcgtaacgt tcttcggtcc gctcgcgggt
     3601 ggaggtatcg agctccacgt tgacgcgcac gtcggcgttg ccagagccca ccacgcgctc
     3661 cacctgggcc agcacatccg actcgtagcg cgtggccatc gtgcgcgctt gctcgcgctg
     3721 tgtgacctga atgtccgtca agtcagaggc gtcttcaccg tcagaaactg gacggtgcaa
     3781 agtggcaccg tccgtgctca cgaccgaaac tcggtccttg gacaggccag gcacggccgc
     3841 gctcacgagg tgcacgatgg tcgcgatctc gcgtcggccg aaggccgccg acggcgcgag
     3901 accaattacg accgatgccg aggcggcctc cgaacgcgcg gtgaacagcc tgcgctctgg
     3961 catcaccagg tgaacccgcg ccgacttcac gccgtggatc gccatcacgc tgcgagacag
     4021 ctcgccttcg agcgctcgcc gcaagctgac ctgttgctcg aattcggtgg ctccgatccg
     4081 cgattgatcg aagatctcga acccgactcg tccgccgcgc ggcagcccac tggatgccag
     4141 ctcgagccgg agcgccgcaa ccttctcctc cggaaccatc aacgcggtgc cggaggcctc
     4201 gatcttgtac gggatttgct gggttttgag cttctctacg atcgcagccg catcttcggt
     4261 gctgagctcc gtgtagaggt aggtgtagtg ctcgcgatta cccaacaccg tgatcgcgga
     4321 gactaacagg agcaccgcgc ccgtgacccc gatcagcgcc acgcgctttc cgaccgaaag
     4381 cttcgcccag aattgggaca ggttctggag cacggttcgc aaacgttcgg gcatcgtggc
     4441 gctctgcgcg ggcttacact cgaagtgctc ggacccgcgt cactgacctt gaaacaggtg
     4501 ccgttgacgg ctgtgggtgg gctccgacta gtattgcgga gcctgttggg acgttctcga
     4561 cccggtcatg aagcgagttt cgtgccatgg aactggcagc ctgctcgccg caacgacgat
     4621 caaatctgca tccgccagat ctcgtaaaag gcgtcgatca cgcggttacg cacgttaccc
     4681 aacacgcgca gctcgatgtc cgctttgctg agcgagacca tcgtaccgtg aatgtcgtcc
     4741 cgtgtacccg ccgcgaacgc ctccccttgg gccgcagcct gttgaatgct ctgattcgcg
     4801 gagaccgcca tgctcttgag caggtcttcg aatacgcccg aagccgccgc gtccgtggct
     4861 tcggcctgtt cggccttggt cggcgtcgct tcgaacgctg cgtgcggcgc gaacagcggg
     4921 aatgtgggtc cttcgatggg caaaccctac gcgcctcctt cgcgtgctga cgccgaagaa
     4981 aaagccacgg ctcgtttcac ttaccgagtt ccagcgcact tcgcgccatc tgtttgacgg
     5041 tctcgatcga cgtaatcccg gcttcgtagg cgcgcgaggc ggtgatcatg ttgaccatct
     5101 cctcgacggc gttgacgttc gggtactcga cataaccggc gggattggcg tcgggatggc
     5161 cgggctcgta caccatcgtt cccggccgtt gatcctcgac taggcgtgac acacgcaccg
     5221 tcgccgcacc gggatcctgc acgttcaagc cacgctcgag cgccaccgct tcgaagacgg
     5281 ggtcgatgcg ccgataaggc ccgccttctt cggtgcgcgt cgtgcgcgcg ttggccaggt
     5341 tggaggagat cgtgttcatg cgcacgcgct ccgcggacag gcctgaagct gagatttcga
     5401 gagcggaaaa gacaccgagc atgggatggg tcctggaaga cgggggcttg cgggtagaac
     5461 aggaaggcgt tcggggagct ggccttcaat cagcctttgc cgtcgctcgc ggcgtaatgc
     5521 agctgcctga gttcggcgga gacgatcgcg gacaccgtgt cgtagcgcag ctggttggcg
     5581 gctactttgg cggcttcgcg gtcgagcgac acgaagttct cgtcgttgcc cgcgcccgca
     5641 gacacgtctc ggaacaactt gccgtgactc agaccttgat tcggagcggg cccctggagg
     5701 tgggccgagt tggtgcgatt catcgaaaca tcgagcacgg ctgcgaatgg ctccgtgtcg
     5761 atgcgcgcca gatccgctgg acgatagccg ggcgtgtcca cgtgcgcgac gttggacgac
     5821 aacaccgaat gacgttcgag gtggtagtcg agggcggcct gaagtgggcc gattcctgaa
     5881 aacacatcag acatgggcaa agagctgctc ggcaattgcc gggggcatcg cttcgctgtt
     5941 cagcacttgc cgtgccaagc cgctattcgt cagcccaagc agacgcggtt ctgttcgcag
     6001 caccggagat gcgaacagcg aaggtaaggc agccgcagtg tgcatgctct ttccacgaat
     6061 tcgatctttt cgttcaaggc ttggtcgcgc cctccgttcc tggccagtga gcgacgccag
     6121 aagctcgatc ccccgcctcg tacccgccca gcccgtgccg ccaagtaccc gctgaccccc
     6181 cgggtggctt gcgacccagc agtcgagcgc tgagtacgac tgacaaagcg cgtgaattcg
     6241 agccgcggcg ccaagatcgc ggcatgctgc ctgagtcccc tcggacccat gtggagcggg
     6301 ccccaatatg ccgcgaaaag cagggcgaaa gcccgaatag gctcgatccg ccaacgatcc
     6361 gacactcggg cagccaatct tccggcgttg gcccaggcct tgctgaatgc agcgtccgaa
     6421 ggcggagcaa tggccaaggg atacgcggta gacacgatgg acgacctatc aatcgaagat
     6481 tcgagctcag gcttgcgtga tctcagcgca atcgcactct gttcgtcagg gccggaagca
     6541 atggaggacg aagagtccat tgccgacgag caaggcgagg aacaagtcga agagcctgtt
     6601 acgaattcag cgcgcattcg cgaatccaag ctgccgcaga gcatgcaagg tgtcgtcggg
     6661 atcgcggaga gcctggtgga ggtgtatcgc atcgtcgatc gcgtcgcaga caccacgtgc
     6721 aacatcctga ttacgggcga gagcggcacc ggaaaagaag tggtcgcacg cgccgtgcac
     6781 cgcgtgagtc ctcgtgctca ggctccgttc gtggcggtca actgcggagc aattccggag
     6841 gcgctgctcg agagcgagct gttcggtcac gccaagggcg cattcaccgg cgcgcatcaa
     6901 tccaagccgg ggcgtgtcgc actcgcacaa ggaggcacgc tgtttctcga cgagatcggc
     6961 gaaatgcccc tgtccctcca ggtcaagctg ctgcgtatcc tgcagtctcg ggaatactcg
     7021 cccgttggcg acaaccgcgt gctcaaggcc gacgtgcgcg tagtggcggc gaccaacgtg
     7081 aacctggaga acgcggtaca ggcgggaacc ttccgcgaag atctgtatta ccgcttgaac
     7141 gtcatccacc tcaccgtgcc gtcgctgcga gagcgccccg aagacgtgcc gctgctcgta
     7201 cagcacttcc tgctgaagtc acgcgagaag ctcgctcgcg gcgcgatttc gatttcgcgc
     7261 gccgccgccc agctgctcgc ggagtatcac tggcccggca acgttcgtga gctcgagaac
     7321 accatcgagc gcgccgtgct gctgtgcgcg ggtggcgtga tcgaacccaa agacttgcct
     7381 cagcgcgtgc tcggcctcgg caccgagaag cgcatcgccg ctcgcctgcc cgactccggt
     7441 ttggatctgc gtaacgcggt ggagaccttc gagaatcaac tgattcggca agcgctcgag
     7501 cgcaccaagt ggaacaagaa acaggccgct accctgctcg gtttgaatcg caccacgctg
     7561 gtggagatgc tcaagcgcaa gcgcatcacg cgcgcggcct gagcctggct caggccttgc
     7621 ttcttagggc tgagtgacga gatccccagc cctggagggc gtgcgccgag tccacggcgc
     7681 gcggcgctcc tccgcgttcg gcgctgccag tgcgctcctg ctcgcgctgc tgtggaccgc
     7741 gcgggctcgc gccgatggct acgccgtaga tgggacgctg ggcgtgggaa gtgggatgca
     7801 agcaggggat gcagggagcg gcatggcctg gtcgcgagcg cgactacgtc ttcaaaccgg
     7861 cttcgatttg atgtccgacg agctccccaa tcaagccgtc ggcttccggg ggttcgtcga
     7921 gctcgagcga cgcggcagcg ttggcctcga ggcacgctat acgcgttggc taggccgggg
     7981 gtttggtgga ttcgtgttcg gcagcgccgc gttcatgccc gagacactgg tgggcggtgg
     8041 ggtgggcttt acgctggtca ttccgctcgg gtcgcgagtt ggtctcggtc tcgagcccgc
     8101 ttttgcagca ttcccgctcg gcagcgactt gcccgagggc agcttcgtgt tcctcgccgg
     8161 cctctctgca ggtgtgcgcg tgcgtctatg aaggtctctc ccgcaatctg gctctctctt
     8221 ctgctcggcg gtcatgccgc gagctgtgcg caacccggtc agtgcttccg acacactgac
     8281 tgcgctcgag gcgagcattg cgagctcgga gcttgcgtca aagactcgac aggaaatagc
     8341 tcgccgggtg acgctggcgc gggaggagag ttctcgggtt cgggcgccgc gcccagcgcc
     8401 gacggcggca agtcgagcgg cgggactcaa gcttcttcga gcaaacccgg gagctccggc
     8461 gcaggtggcg ctggcggtgc gggcggcgaa gcggcggaca gcggcgcggg cggatgaatc
     8521 ggctctggca cccgagttgc agaaagcgag cgttagcccc atgtttcgct cgcttcacat
     8581 cgccgcaacc ggaatggccg cgcaggagac gcacctcgag agcatctcca acaacatcgc
     8641 caacggtaac accgtcggct tcaagaagca gcgcgtagac ttccaggacc tgctgtacca
     8701 aaccgtgcgc cagccgggcg cacctaccgg ggcgaccacg acgtctccca ccgggctgca
     8761 gctcggctcg ggggttcgag tcgtgagcac ggcccgcatt tacgagcagg gcacggtcct
     8821 caccaccaag aacccactgg acgtcgcgat cgagggcgac ggcttcttcg tcgtgcaaca
     8881 gaacgatggc aagcccggct acacgcgagc cggtgcgttg cggaccgatc cgcagggacg
     8941 catcgtgact cccgagggca tgccgctcga cccgccgatc tcggtcccgc aaggcgcgac
     9001 caacgtcagc atctccgcca atggccgcgt cagcgtcacc ctgccgggcg agaaagatcc
     9061 cgcagaaatc ggccaaattc agatcgcgac cttcatcaac ccttctggtt tgcaagccca
     9121 aggtcacaac gtactcacgg cgagcgcggc cagcggcgac gcccaagtgg gtgagcccgg
     9181 caccgaggga cgaggcagcc tcttgcaagg cgcggtggaa cagtcgaacg tcgacatcgt
     9241 cgaggagatg atcggattga tcagcgccca gcgctcctac gagatcaact ccaaggtcat
     9301 cactacggcc gacgacatgc tgcgcgctgc cactcaaatc cgatgatcca cctatcctac
     9361 ccggagcatc ggcgcggccg acgcgcggcc ttgctcggtg tcgtactcgg cttggtgaca
     9421 ctctcgagtt cggccgtcgg cgagccgatt cgagtcgtcg accatccgcg tgttcgtctg
     9481 cgcgatttga gcacggccct ggccgccctg cccgccccgc tcccagaagt agacctcgga
     9541 ccttcgccgc cgccgggcgg ctcacgacta ttcgcgagag agagcgtgct ggccgagctc
     9601 cgcacgcaag gtctcgagcc gaaaggtgtc aatttacccc cggtcgtgcg cgtacagcgc
     9661 gcagcgcgcc acatcgagcc gagtgagctg aaggctctgc tcgagcctca gctgctggcc
     9721 gcgctcggcc cgaacatgaa gctcgcgagc ttcgacatca agaagggcct cttgacggtg
     9781 ccgaacccgg aagtcgggcc cgtgcgcttg ccccaattct cgcggcgtga aggcccaatc
     9841 caactaacgg tcgttgcgga cctcctgcat gacggcaacg tggtagcgcg tctaccggtg
     9901 ccgatcgtga tcgagctctc cgccgccgcg ctccaaccga gcgtagccaa aggtgctcgc
     9961 gtcgacctcg tcatcgagcg cggtaatgcg cgcatctccg cggcagccac cgcgctcgaa
    10021 gcggcggagc tcggccaggt aattgctttc aaggtcagca cgacctcgcg cgtgctgcgc
    10081 gcccgcatca cctcccctac ctctgcccta gtggtctcgc cttgaatagc tctcgtttcc
    10141 ttctgctcgc gctcgggctg atggcctgtg gcccggccca tatcgcgccg ttcacgccca
    10201 aacagcggaa gtacgagccc ggcgcgtacg ccgccaccca aaagggctcg cagccggcga
    10261 ccggctcgat ctacagcgaa gcgcaacccg gattcctgga agacacgcgc gcattgcgcg
    10321 tgggtgacac cgtgctggtg cgcatcaacg agcatgctga cgcgcagggc aacgccacta
    10381 ccaacctcaa caaagagtcg aaccgtgagg tgggcgccga agccatgctc gggctggtcc
    10441 cggcgatccg caaatcctat cccgacatag atccgagcaa gctgctgtcg ctggcgtcga
    10501 gcttcgactt caaaggccag ggcagcaccc aacgcgcggg cacgttgacc gcggccatcg
    10561 gcgtgcacgt caagcaggag ctcccgaacg gggacctgtt cgtcgagggc accaaggtcg
    10621 tgatgatcaa tcacgaggag tatcacctgt acatctccgg tgtgcttcgc ccggcggaca
    10681 tcgagcaaga taactccgtg gactcgactt tgatcgcgga cgcgcgcgtc gagttcaccg
    10741 gtcgcggaga catcgacgac caagtcaacc gcggctggct cacgaagctc ctcgacttcg
    10801 tcaatccctt ctgatccaca gcatccatgt ccccatctcg gttgctcccg gctcttcgcc
    10861 tgctcgcgtt ctggtgcgtc gcgtttggcg tccttctttc gacgcgcgcg gcacgcgccg
    10921 atcacttgcg cgatctgtgc gacgtggtcg gggtacgaga aaaccagctc gtgggctacg
    10981 gtgtggtcac gggcctgaac ggcacgggtg acgatctatc ggcgcctttt gccactcagt
    11041 cgttgagggc cctgctccga cgcatcgggg tacaggtgga ctccaaacag atccgtctga
    11101 aaaacatcgc ggccgtattg gtcaccgcaa atatccccgc cttcgcgcgg ccgggctcca
    11161 agctcgacgt caccatctct tcaatcggta actcgaagtc actgcgtggc ggcgtactcg
    11221 tacaaacccc gctgcgcggc gcagaccgca agacttacgc ggtcgctcaa ggcgcgttgc
    11281 tgctgggcgg cttctcggcg ggcggctccg gtgcatcggt gcaagacggc gtgaccacca
    11341 ccgctcggct cccgaacgga gcgctcgtgg aacgcgagat cccgacgacc ttcgcgaacc
    11401 agggcaaagt cacgctcgcc ctcaaatcgc cggacttttc tacggcacag cgcatcgtcg
    11461 ccgcgctcga gaaggagctc ggcgaaggca acgcgcgcgc gctggacggt ggcgccatcg
    11521 aagtcaaagc gcccaaggaa ttggcgtcca agccggtcga actgatcgct cgcttgtccg
    11581 aggtcgaggt cgatccagtc accgcggctc gcgtcgtcat caacgaacgc acgggcaccg
    11641 tggtcgcggg aggcgacgtg cgcctctcgc cggtggccat cgcacaaggc ggcatcacca
    11701 tctccgtgca agagactcgc accatcagcc aacccggccc tctctccggc gggcagaccc
    11761 gcgaacaaca gaacacccaa gtcaccgcgg aagagggacg acccgcccag actctctcct
    11821 acttgaaggg agcaacgacg ctcgccgaag ttgctcaggc gctctccacg ttcggtatct
    11881 caccgcgcga actcgcgagt atcttgcagg cgctcaagac cgctggcgcg cttcgtgcag
    11941 agatcgtggt ccagtagtga cggtgcagta atgattggac ctctcaacgc cctacgagct
    12001 gaaagcgcgg ccgcgacgac gagtacgtcg agcggaacca atgccgcaac gcctcgcccc
    12061 gaggtactgc gggccgcccg tgacttcgaa gccatcttct tgcgtacctt gctcaaaccg
    12121 ctcgagaaca cgactcgcct cagcaccggt ggccagagct tgcctgggca gtccgcgtac
    12181 ggctcgatgg tggtgggagc gctctccgac agcctcgcgg cctcgggtgg gctcggcctc
    12241 gcgggcgtga tcgctcgcgc catgaccgcc tccgatccgg cgtccgcgct gcccccatcc
    12301 gcgggtcaac ccggctcacc ctcgtccacg tctgcggaac cgtcaccgga tgtaaccaga
    12361 tccccgcgcc tggcctcgcc aggtgccccc acaaacattg aagcctcaag tgctcgctcg
    12421 gggctcaagt aatggaaaat cgtaaccgta tccgagcctt tgctgaacga aagctcgcgt
    12481 ctcaggtttc tctcaagggt tcgcgcgcct tgccgttcat ccagtcgaga tcctatgggt
    12541 cgagtatcac cgcgacccag ctccggagaa ctgcatgaaa ggtatcaccg gtaacccagc
    12601 tctagacgcc tatcatcgga tgggcatcag cccggtcggc aacgcacgcc ccgccgaacg
    12661 ggtccaaggt ggctcgcagg ccgccgtcag cagtcctgca gcccaagtga cgatttcggc
    12721 acgcgcccgt gacttggcca gcgagtccgc aggcagtgtc gatcccagca aggtggaaga
    12781 gcttcgcgat cgcatcgcga gcggcaactt ccaggtcgat ccgcgcgctg tcgccaacaa
    12841 gatgatggaa cagctcggat aacgaatcag aaaggagtgc gcttcgccgc gacccgaggc
    12901 cacacgaatg tccgaaccac tgctcgatga cgatcccgcg agtacgcttt ctgccctgga
    12961 agcgctacac gaggaggagt tgtccgtgct gagaaacatg gatcgcgcct ccctcgacga
    13021 tctcactgct cgcaaagaac agctgtgcga gcagctgcgc gccgcccagc tgagcgggaa
    13081 gttaaccgac cgccaccgca gccaaatcga acgcattcgg cagaaggcgg cccacaacca
    13141 gctgctcgtg gtccacgccc gcgatgcagt gcgcggcatt ctttctcaag ccacgggcgt
    13201 ttgtctggac gttctgccgt cggggcgccg cggctcgact caagagggtt tgcgcctgaa
    13261 tgtgaggggg taatgtccac gctctcccaa ctcctatcga ccgcacgaga tgggctcacg
    13321 gcccagtcct acgggctggg agtcactggc cagaacatct cgaacgtcaa cacgcccggc
    13381 tacgtgcgtc gcgaagccat gctcgagact cgagccctgg gcacgcagac caccggcagc
    13441 gtagaggcgg taggcctacg gcgggtcacc gatacgttct tggagcgtcg ccagttcgaa
    13501 gcgaccggcc tgagctccgc cgcatccgaa cacgactcgc gactggcgtc gttggaagcg
    13561 ttgttcaacg acagcgcggg caccggcttg ggtggcgccg tggatgcgat gttcggctcg
    13621 ttctcgcgac tggctgcgag cccgtcggac tccacggtgc gccaatcggt gttggatagc
    13681 gccagcaatc tggcgcagcg cgtgcgcgac accgccaaca cgatcgccaa gggtcgccag
    13741 gatctgctgc aagatgcgca ggctaccact actcaggtca atgatcgctc gcgcgcgatc
    13801 gcacgcctga acacccaaat ctctgcggcg gagacccaag gcaacgatgc agccgatctg
    13861 aaggatcagc gtaaccgctt gctgctggag ctcgccagca tggtggacgt ccgcacgatt
    13921 gtctccggca gcggcatcat ggtgcaagcc tccgggacca ccctcgtcga aggcggcgag
    13981 gcgcgcgagc tgagcatctc ggtagactcg agctcgcgga tgcaaatcct cgcgtcgcgt
    14041 tcggatgggc cacccaccga cgtcactcgc ttcctcaccg gcggaaagct cgccggcatc
    14101 cgtgaggcac gcgacgtaga cttgttggag gtgggccaga agctcgacca attcgtgttc
    14161 gacgtcgcga ccgccgtcaa cgaccagcac cgtcaaggtt ttggcctcga tggcgccacg
    14221 ggacgcgaca tcttcgcggt caccacggtg cccgagggtg ccgcacgcgc actcacgctg
    14281 agcgcggatg ttgccggcaa cccgaacgcg ctcgccgctt cgagctctgc gggggcactg
    14341 ccgggcaact ccgacaacgc ggccgcactc tcggacttgg caagtacacc cgtcgtattc
    14401 ggcgccaagc gcaccgcggc cgaaggctac ggtgacatcg tgggcgatgt ggctacacgg
    14461 cgtgccgaca gccgtcgcat ggtcgaatca cgcgaggcca tcagcgcgca gattgacgcc
    14521 atgcacgaag ccatgagcgg ggtttccttg gacgaggaat tcgtcaatct gaccaagttt
    14581 cagcgcgcct atcaggcttc ggcccgtgtg ctcacgacga cggacgagat cttgcaagag
    14641 ctggtcaact tgaagcgctg agctcgaact ggctcggtgc aggttgacga gccatgcgga
    14701 tcaccgacgg cctacgctat caatccgtcc agcgcaatct cacgcgcatt cagaaagacg
    14761 ccgcggaagc gtcacaacaa gcctccacgg gccagcgttt gggtcagcct tccgacgatc
    14821 cgattgcggc cgccgagctc tcgcgcgtgc gcgcctcgtt gaacacggcg gccgcccaaa
    14881 gggacgccat cgccatggtt cggggagacg ccgagttggc cgagtccaca ttgtctcagg
    14941 tgggcgagct gatgaagcgc ttgcgcgagc tcgccatgca gggtgcgaac ggctcactca
    15001 gcgccgaaga ccgtgacgcg ctcgccaccg aaacgcgcgg catcaaaacc gaggtgctac
    15061 gcctggccaa cacccggggc acgcgagggt acttgttcgc gggcgctcgc accgacagcc
    15121 ccgcgttcga cgcggcgggc gcatttcagg gcgacgacag cacccagctg gtggacatcg
    15181 gggccaccac tcctacggcc gtaagcgcga gtggagcgca agcctttacc gcggcgggag
    15241 ggcgtgatgt gttcgcagat ctcgacgcct tggccaacgc gctcgacgcg aatgaccccg
    15301 ccgcgaccgc ccagagtctg gacgcgctcg acgcctccga gcgccaggtc ttgaaggaac
    15361 gagcaaggat tggactcgtc gtcgacaaac tcgacaccag caacggcgta ttggagcagc
    15421 tggacacgga tctgcaaaag cgtggcgacg cgctcggcac ggccgacgct ttcgaagcct
    15481 attcacgtat gaccgtactg agtcagtccc tggagcgcgc cgtcgcggtc tcccggcaga
    15541 tgctcgaatc cgcgagctat tggaaccggt aacgaactcg actcgtcgca agcccgaaat
    15601 cgacccactg ggctcgaggc gagcggtggg taaccgcaaa cttccggaaa cacggctgaa
    15661 gctgaggcgc aggtctactt acgtactagg agtgatgagc agccctgcct acccagaccc
    15721 acgacccgaa agagcctcgc aggctccgcc gtcctccgcg ccgcgctccg cgggattttc
    15781 cgccaacctc gagggagcga gcctcgcgga tctcgttcag atggcgtgcc agtccggtct
    15841 gcagagtgtg gctcggatcc gctccgcgga acaagtcggt tacctttact tcagccaggg
    15901 caggttggtg cacgccgtat ctcccaattc agttggagac gaggcggcgc tcgagatcct
    15961 gagttggcaa gcggggcgct tcgagccgtg tggaatcgag ccgtccgcgc acgtaaccat
    16021 tcagtcgagc taccaaagcc tcctgatgcg cgcggcacac gcgcgcgacg agcgtgcacg
    16081 cggagaaccg gggcgcaccg actccgcgcc gcgcaaggtg atcaccctgc cccccccacg
    16141 cccacgcgag actgccgcgt cgatgcctga agtgcgcgac gggagtccgc gagagccgag
    16201 caagaccatg ccttcattgc gggacgatga gcgcaacgag gaggcgcgcg ccgagagcag
    16261 gctctcgtcc gccgagcggc cccgcggggg ccccagaaac acggccgcca aggcgaccgt
    16321 gcgtttggac gcggaaggca atctgcgttt cgcgcaaggt tcgggaaccg aggaattggc
    16381 ccaggttgcc gcgctcgtga cccggctcgg caacttggtc gggcgagcgc ttggcctcga
    16441 tcgcttgcac gcggtggaag gcaccaccgc ctcgggtcga acgttgatcg tgatcgagaa
    16501 gcaaggagaa gtgatcggcg ttcgcacggg gccagaggac gacgtcagca cctttcgcaa
    16561 gacctacggg ttatgaactc cactcccttg gatggtgcgg tgaactggca atgacagccc
    16621 ctgcgttgag cccagccgtg ttgcacggtc tgcgtgacgt cgagggtgtg ctcggcagct
    16681 tcatcttgag cgagacgggc gagctcttgc tacgcgacct gcccggcatc ttcgacaatg
    16741 agttgttcta cgaggcgggg ccgcggctca cacgcctgtg tgatgcgctc tctgcgcagg
    16801 gggaaccgct ggaggcagcc ggactgcgct acgcagagca caagcttcac ttgcgacgcg
    16861 cgcaggggtg catcctgtgc gtgctctcca cggctcaggc gaaccccccc gccctaaaga
    16921 tggccctgtc attgctggcg cgtcgcctcg agatccgcgc cgcggaactt agggcacccg
    16981 agatgacggc gagccctcag tcgccacttc cctctggcgc ggggaaaccg gcttcggcgc
    17041 caccgagtgg tgcgcagaaa tcgaatggcc agggcatcgt tccgggtacc ggccactccg
    17101 tgcatggctg ggggccctct tccggtcaga gcggcgcgcg tcagagtgag cctcccccga
    17161 atgggacggc gcgcgtgatt cgcacgtacc gcggccgtcc catcgagtaa ggaactcgag
    17221 cttcgcaata atcactgcat gcggacattg tcgatcgcga agtcgagcca gttgtcccgc
    17281 acctgaatct cgaagtccaa gcgaatctgc atacctcgga atgccgagat atccacggtc
    17341 ttcgtaatca tcgcagactg cgaagcggcc tcactcgtgg tcttgtaaag ctgctgcagc
    17401 actgtgttgg tcttggggct gcgcacatag accgcgatat attggcgagt ggcgttgaat
    17461 gtaccgaagg tgttgatgta gcgcatatcc cacttcagcg aagtgacccc agacggcaca
    17521 ttgaagtctt gaatcaagtg ggaattcgcc acgccggtct gatagttcac cgccacccgc
    17581 gtgccgtccg tcgcatccgc ggtgtacgga agcgcttccg acgtgagttg gctattttgc
    17641 tgcgtgacgt agttcaggat gctcgaccct tggttcaagg tcgtgccttc cgtgaccaac
    17701 ccccaaattc cacccgtcac cgtccaacct tggaaatttg cgagttcgaa gctgccgttg
    17761 atgaacgggt cgtagtcgca aacgtagctc gtgctttgaa tttcggtcgc atcgagcacg
    17821 ccactggcgt tcgaatccag accggcatcg atgcgccggc cgcccccggt gcagttgacg
    17881 ccgctagcct cggcactgag cttgaccaag ctattgacgg cattgccggc tgacgtgccg
    17941 ttcgtcccgt tggtgcctgg cgcgccgttg cagacgtagc gagttgcgtc gatctctccg
    18001 ggcgccagta cagagtcgga gttatcgtcg agcccgtagg agatgcggtt gcccccgaag
    18061 aggcagttcg ggctcgcggg ttcggcggcc accgtaacca gagtgcgtcg agcgtccgtc
    18121 ccggccgcgc ccaccgtgcc gttggtaccg gcaacaccgt tgcaaatata gcgagtgccc
    18181 tcgatctcct cggcgtcgag ggtcccatct tcgtcgtcgt ccaagccaaa gctcacggct
    18241 tggccgcccg ccgtgcagtt cgtgccggcg ggttcctcgg tcaccgaggt aaggcccaag
    18301 agcgcgttta ccccgttgct tccgtccaga gccgagctcc cataacagac gaaatcggta
    18361 cgcgtcactt caccagcatc gagcatcccg ttggagttgg agtcgagacc actctggatg
    18421 ctctggccac cggcgggaca gttttccccc ggcaactcgc tgccggtttt cacgacggcc
    18481 agcaccgcga ggcgaccggg ctcccccgga tcaccggacc cgccgtcaca cacgtactcg
    18541 gtctgcgtta tctcgctgcc cgtcaggcct ccgctcgcgt ccgcgtcgaa gccaatctcg
    18601 atgcgctgac cgccgttcgc gcagtgggtg cccgccgcct ccggactctt gatgaccgtc
    18661 ggaacgagtg cgctctggcc ggccgtacct tgagcaccgg gagcccccgc gcagacgtac
    18721 tgggtattca gaatctctgc gtcttgcaga atatggtccg cgttttgatc gagccccacg
    18781 tccacgcgtc gaccgccgcc cgcgcatctc accccggcgg gctccgtcgc aacgttcacc
    18841 aagctcacgc gcgcaggcgc gcccggagag cgggtgcata cgtagcgagt ggtcgtgatt
    18901 tccgccggat ccagcgcgtt attgtggtcg agatcgagcc cagattcgat ccgctcaccg
    18961 ccggccccac agagctcgcc tgcggccacc ggagttaccg tgaacagcgg aacagctggc
    19021 gtaacccccg gagccccgcg gcaaacgtac cggacctgag cgacctcgcc ctcgtccaga
    19081 atcccgttgg tgttcgcgtc gactccataa accacgcgtt ggccgccgta ggcgcacttg
    19141 tccccgggcc cctcctcacc gctcacgacc aagctcccca cgccggaatc gccactttga
    19201 cccgcgacgc cgtcgcagac gtagtgcgat tcccgagcga cctcggtatc gatcgagccg
    19261 tcctgatcga ggtccgggcc gatctcgact aactgcccgc cgtgttcgca gttcttgccc
    19321 ggagcttcct cgcttaccgc gacgagcatg agcatgccat cccgaccgtc ttcaccgggc
    19381 tgacccgcgg gcgcttgcgc tggctctcgg cccgtcgcgc cgtcatcccc gttgcagatg
    19441 tcccggtgcc c
//
LOCUS       DASTKU010000861         7039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_168354, whole genome shotgun sequence.
ACCESSION   DASTKU010000861 DASTKU010000000
VERSION     DASTKU010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168354"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="VFQ61_30345"
     CDS             <1..1607
                     /locus_tag="VFQ61_30345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958847.1"
                     /translation="VPPELERNVDLDWMRLSLADAESGIRPKDVERAARRMPPGAYRV
                     VTTLEQTGLLQRCARGTQLKLEPTWLRVFLTHHAVQAVVARSPFEWGEALLRGHAAVQ
                     VTEALLSRTVQETGACLEPVLDLEAKDQPAFAAAVDVAFRVAGIARLLRVDVSSEVLE
                     GLWRTQGELVLDLGTGLPVPRIELRPTSIWADAGAPEARFLLEPGTFYLAALSIAETL
                     DRASVSALSLFDPFHQTAPNPALGAAYDAIAAAVPQFVPWRSAMLSLISRVRSCVGNV
                     NAWDQPHSLEMPAEIIDEFLHGVLGFATLDRAMGFPGWLEAVLTLCEERGVVPGELAR
                     AFWTAWNAAERPDRADFLAPRAPYADLFWANIPEALLAAMLMDARIEAVPYHAFQEEQ
                     WYAFSRALRGAPSLVREHEAWAQMPVKWVTELCLLPLEWELAPRSVEVLWRRFPELLE
                     RAVWASLASATSEAAQASQALLQSAPREVAISLAERLAPRMTSLSPGASRALRSLLRR
                     LVAERVPEWRRAYKSLAELEQISFKL"
     gene            1611..2627
                     /locus_tag="VFQ61_30350"
     CDS             1611..2627
                     /locus_tag="VFQ61_30350"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET9958848.1"
                     /translation="MRAIIVEQRFGLDQVRLAEIPMPALGPGQLRIRVRAASLNYRDI
                     LVARGDYNPNYPLPLILGSDAVGEVCEHGPGVDQTEFPLGSRVCPTFAQGWLDGPPLR
                     NTVRQTLGGPLPGVFAEFAVLRSDGLIALPEDVSDEAAACLPCAGVTAWSALCRHAEL
                     RAGQSVLVLGSGGVSCFAISIARSLGLRVFATTRDLANKGNKLRELGADQVVDAQTAG
                     WGRRVRELSGGEGVDHVIEVSGGATFAESLAALRPGGGISLIGVLGGRAAPLDVLPIV
                     MRNLRVQGIFVGNRADFLALLGSISSGVRPVVDSVWPFERFSEAIQHMLNGDHVGKVV
                     LRWS"
     gene            complement(2683..3210)
                     /locus_tag="VFQ61_30355"
     CDS             complement(2683..3210)
                     /locus_tag="VFQ61_30355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958849.1"
                     /translation="MNGKSSGSSSVVVLEKDADFPHWVADYQRQATNSVVVSHMGGET
                     LADFEARIERRVAELTEELRVAVISCSRFRRGERFSQDDQGAARERISRMLLARMPAR
                     GQCELFLVPSTHTDEDEKHAIFELAGALCDDLRGTSRSVRVRFSFGRPESGILPRLAD
                     TQPELLANRAVARRG"
     gene            complement(3808..5211)
                     /locus_tag="VFQ61_30360"
     CDS             complement(3808..5211)
                     /locus_tag="VFQ61_30360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9958850.1"
                     /translation="MTLEVGQLIEGKYRIARLIGEGGMGAVYEGENVRINRRVAIKVL
                     HAAFTGNREVLQRFEREAQAAGRIGNDHILDVLDLGTLPNGDSFIVMEYLDGEPLSER
                     IKKLGRMSPEQLTPIMRQVLIGLGAAHQAGIVHRDLKPDNIFILKEKAGKSDFVKIID
                     FGISKFQPMTGEGMKMTRTGAVMGTPYYMSPEQASGSHEADQRSDLYSIGVIMFEAVT
                     GRVPFDAATFNQLLFQIVLADVPTVESTVPDIDRAYSSVISKAMARDMNLRFQTAAEF
                     VRALDAWLATGSAVSIPPPVDGATAGLVPKGRASFSDAAGLHGSRTAGSWAKSQPSEP
                     SHKTNSKRLAIGLGAGAALLCVGIIAGVRALQSPITAPTDVTKTSQPVNTAPAAPPHA
                     TEPQSPSMGTSPTPPVPEAAKPVESAADPSAGVPAPSSKVAPTKTATAPAARPPRRPA
                     GTPRPNAGKPTTPDFGY"
     gene            complement(5785..6978)
                     /gene="lpdA"
                     /locus_tag="VFQ61_30365"
                     /pseudo
     CDS             complement(5785..6978)
                     /gene="lpdA"
                     /locus_tag="VFQ61_30365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
BASE COUNT         1178 a         2158 c         2271 g         1432 t
ORIGIN      
        1 tggtgccgcc ggagctcgaa cgcaacgtcg acctggactg gatgcgtttg tcgctcgctg
       61 acgcagagag tggcatacgc cccaaggacg ttgagcgcgc cgctcgccgc atgccccccg
      121 gagcgtaccg cgtagtgacg acactggagc aaaccgggtt gctccagcgc tgcgcgcgcg
      181 gcacccagct gaaactggag ccgacgtggt tgagggtctt cctcactcat cacgcggtgc
      241 aggccgtggt ggctcgctcc ccgttcgagt ggggagaagc attgctgcgt ggccacgcgg
      301 cggtgcaggt caccgaggct ctgctttcgc gcacggttca ggagactggc gcctgcctcg
      361 agccggtgct cgatttggag gccaaggatc agcccgcgtt cgcagccgcc gtcgatgtgg
      421 cgtttcgggt tgccggaatc gctcggctat tgcgggtcga cgtatccagc gaagtgctcg
      481 aaggattgtg gcgtacgcag ggcgagctgg ttttggatct gggcacgggg ctgccggtgc
      541 cccgcatcga gctaaggccc acttccatct gggcggatgc gggtgcccca gaagcgcgtt
      601 ttctgctgga acctggcacg ttttacctcg cggcgcttag cattgcggag acgctggatc
      661 gcgcttcggt cagtgctctt tccctattcg atccttttca ccaaactgcg cccaatcccg
      721 cgttaggggc tgcctacgat gcgattgcgg cggccgtgcc gcagttcgtt ccctggcgta
      781 gcgcgatgtt gtccttgatc tcacgggtgc gcagctgcgt cggaaacgtg aatgcctggg
      841 accagcctca ctccctcgag atgcccgcgg aaattatcga cgaatttctt catggtgtac
      901 tcgggtttgc gacgctcgat cgagccatgg gctttcctgg ttggctcgag gccgtgctga
      961 cgttgtgcga ggagcgaggc gttgtgccgg gagagttggc gcgggccttt tggacggcgt
     1021 ggaacgctgc agaacgcccg gatcgagcgg actttctggc cccgcgtgcg ccgtatgcgg
     1081 acttgttctg ggccaacatt ccggaggcat tgttggccgc catgttgatg gatgcgcgga
     1141 tcgaggccgt gccgtaccac gcgttccaag aggaacagtg gtacgcattt tcacgggcgc
     1201 tgcgcggagc tccgagcttg gtccgagagc acgaagcgtg ggcgcagatg cccgtgaaat
     1261 gggtcaccga gctgtgtctg ctgcccctcg agtgggagct tgctccgcgc tcggtcgagg
     1321 tgttgtggcg ccgattcccc gagctccttg aacgcgcagt gtgggcttca ctcgctagcg
     1381 ctaccagcga ggccgcgcag gccagtcagg ctttactgca gtccgcgcca cgcgaagtcg
     1441 caatatctct cgcggagcgg ctcgcaccac ggatgacgag cctatcgccc ggcgcgtcgc
     1501 gcgctttacg aagcctgctc aggcgtctgg ttgcggagcg ggttccggag tggcgccggg
     1561 cttacaagag ccttgccgag ctcgagcaga tatcgttcaa actgtgaatc atgcgagcga
     1621 tcatcgtcga gcagcggttt gggttggatc aggtgaggct cgcggagatc ccgatgccgg
     1681 cactcggtcc ggggcagctg cgaattcggg tgcgagcggc atcgttgaat taccgcgata
     1741 tcctggttgc tcgtggtgac tacaatccga actatcctct gccattgata cttgggtcag
     1801 acgccgtggg ggaggtatgc gagcacggac cgggtgtgga tcagactgaa ttccccttgg
     1861 gtagccgcgt ttgcccgacc ttcgcgcaag gctggttgga tggccctccc ctgaggaaca
     1921 ccgttaggca aaccttggga gggccgctgc cgggcgtctt tgcagagttc gcggtactcc
     1981 gctcggacgg cctgatcgct ctccccgagg acgtgtcgga cgaagcggcg gcgtgcttgc
     2041 cctgcgcagg ggtgacggct tggagcgcac tgtgtcggca tgccgagtta cgagcaggtc
     2101 aaagtgtgct cgtgttggga agcggcggtg tctcgtgctt tgcgatctcg attgctcgaa
     2161 gcttggggtt acgcgtcttc gcgacgactc gcgatctcgc gaacaaggga aacaagttgc
     2221 gcgaactcgg cgcggaccaa gtggtggacg cgcagacggc ggggtggggg cgccgcgtgc
     2281 gggagctgag tggcggggag ggcgtcgatc acgtgatcga ggtgtcgggc ggagctacct
     2341 tcgcggaatc gctcgcggcg ctgcgcccag gcggcggcat tagcctaatc ggcgtgctgg
     2401 gtggtcgtgc cgcgccgctc gacgtgctgc cgatcgtcat gcgcaacctg cgcgtgcaag
     2461 gcatcttcgt gggcaaccgc gcagattttc tggcgctgct cggatctatt tcttcagggg
     2521 tacggcccgt cgtcgactcc gtatggccct tcgagcgttt ctcagaggcc attcagcaca
     2581 tgttgaatgg cgatcacgtc ggcaaagtag tgttgcggtg gagctgaccc caatatccgc
     2641 accggtaacc ggcgccactc tgcgcgccac ccggggctag tttcacccgc gccgcgcaac
     2701 ggcgcgattg gcgagcaact ccggctgggt atcggccaag cgcggcaaaa tgccgctctc
     2761 gggtcgcccg aaggagaaac gcacgcgcac agagcgactg gtgccacgca aatcgtcgca
     2821 cagcgcaccc gcgagctcga aaatggcgtg tttctcgtct tcgtcggtgt gcgtgctcgg
     2881 taccaggaat agctcgcact gacctcgagc aggcatccgc gcgagcagca tcctggatat
     2941 acgctcacgc gcagcgccct gatcgtcttg gctgaagcgc tcaccacgcc ggaatcgaga
     3001 gcatgaaatc acggcaaccc ggagttcttc cgtgagctcc gcgactcgtc gctcgatgcg
     3061 ggcttcgaag tccgcgagtg tctcgccgcc catatgcgac accacgacgg aattcgtcgc
     3121 ctggcgttga tagtccgcaa cccaatgcgg gaaatcagcg tctttctcca acacgaccac
     3181 agagctcgag ccgctgcttt tcccgttcat tacgttccct tcgctcgaca gttcgagcgc
     3241 ctcctgcctt caaaatcttc cgagccgacg tttgctaagc gcgggtgtcc cgagacgcgc
     3301 ttccccgatc ggtacatgag cagcgggcgc ctccatcgcc ccactacctc tgcaccagtc
     3361 agcggggcga tagagtcttc aaaaatcgcc cttgagtcaa ctcgatagtg tgatcgtgcg
     3421 aaaagaggtg attgaggttc catgtgagtc tcgctgctcg cgtcgacatc gatttcccac
     3481 taggtaagca agcggcgttc taggccgctg tttggcgaaa tatttgctgg tcgcgaggat
     3541 ctgcgtaacg ccgcggcggg tcccgggaga ctcagcaaat atcgtattgg gacttgggcg
     3601 ttacactgcg actcggccgg tgcccgcgcg gggtgagcac ggagcaacac gcacccgcga
     3661 cgccacgagc gtaattcgag cgctcgcaaa gctcccagtt ttgggccccg attcgtacgg
     3721 agcttcccca cttcggtgca cgatcgaata cgctgtcgtt acgctacctc gcggctcggg
     3781 ccgtagcgag tagcgctcga gcgacgctca atatccgaaa tcaggcgtcg tcggtttgcc
     3841 tgcgttggga cgaggcgtgc ctgcggggcg ccgcgggggc cgggccgccg gcgcggtcgc
     3901 agtctttgtt ggggcaactt tgctgctggg cgccggaacc ccggcgctgg ggtctgctgc
     3961 gctctccacc ggtttcgcag cttcggggac cggcggcgtt ggcgaggtcc ccattgatgg
     4021 agactgcggt tcggtagcat ggggcggcgc cgcgggtgcg gtgttaactg gctgcgacgt
     4081 cttggtgacg tccgtcggcg cggttatcgg tgattgcagc gcgcgcaccc ccgcgatgat
     4141 gccgacgcag agcaacgcgg cgccggcgcc cagcccaatc gcaagccgct tggagttcgt
     4201 tttgtgagac ggctcggacg gttgagattt ggcccatgaa cccgccgttc gggagccgtg
     4261 aagacccgcg gcgtctgaga aagatgctcg ccctttggga accaaacccg ccgtcgcgcc
     4321 atcgaccggg ggagggatgc tcacggcgct gccggttgcg agccacgcat cgagcgcgcg
     4381 aacaaactcg gccgcggttt ggaaccgtaa gttcatgtca cgcgccatcg ctttcgagat
     4441 aacgctcgag taggcgcgat cgatatctgg aacggtgctc tccaccgtcg gcacgtccgc
     4501 cagcacgatc tggaagagca attgattgaa tgtggcggcg tcgaacggga ctcgtcccgt
     4561 taccgcttcg aacataatca cgccgatcga atagaggtcg ctgcgctgat ctgcctcgtg
     4621 cgagccgctc gcctgctcgg gtgacatgta atagggcgtc cccatcactg cccccgtgcg
     4681 agtcattttc atgccttcgc cggtcatcgg ctgaaacttg gaaatcccga aatcgatgat
     4741 cttcacgaag tctgatttgc cggccttttc cttcagaatg aagatattgt cgggcttcag
     4801 atcgcggtga acgatgccgg cttggtgcgc ggcgcccagc ccaatcaaga cctgccgcat
     4861 tatcggcgtc agctgctcgg ggctcatccg accgagcttc ttgatgcgtt cgctcagggg
     4921 ttcgccgtcg aggtactcca tgacgatgaa tgaatcgccg tttgggagcg tccccaggtc
     4981 gagcacatcg aggatatgat cgttgccgat gcggcccgcg gcctgcgctt ctcgctcgaa
     5041 gcgttgcagt acttcgcggt tgcccgtaaa cgccgcgtgt aagactttga ttgcgacccg
     5101 gcggttgatg cggacatttt cgccctcata aacggcgccc atgccgcctt cgccgatcag
     5161 ccgagcgatc cgatacttgc cttcgatgag ctgcccgact tccaaagtca tgggaacgag
     5221 ttccttctgc gcacgcgcta gttcaagaat acgacagaat cagagccatc tccctcatat
     5281 tttcgagcgc tccgggagct ggtgagggct tcgaggcgcc ttggttgcaa acgccgtggg
     5341 tggtgatcgg ctcgcttggg ggtatgtaga ccaggagctc gggcagtgta gtttcgaagg
     5401 gttatcgctt cggaccgctt gtcgtgacaa tcaccggagg gctcgagata tgagtaggat
     5461 ttcctctctt gagtgggtgc aagaggtggg gatttttcag gcgagtttgt gctgggacgg
     5521 gtggcgcggg ttccagcgtc gcgctggggc tcgagtttct gaggaggagc ccgcccgcgc
     5581 cccgtcttcg attctgcgca gatgtagggg tggctcgaac acacaaaagc actagcccag
     5641 ccggttcacg gggaactcat gagctgctgc agacttccga gttgccgact ctgcaggaca
     5701 aaagccgaga ggccttcctt tgcgggaagg cctctgggtc gcctccacac gaggcaaacg
     5761 aatgcgagcg ggtgacggct taggtcagat gtgtagcgct cgcccggcga ccgccaacgc
     5821 cgcttccttc atggcctccg caagcgtggg gtgcgcgtgc gccgaacgcg cgacgtcttc
     5881 gctcgtggcg ccgaactcga tcgcgacggc agcttcggcg atcaagtccc ccgcgcgagg
     5941 gccgatcacg tgcacgccga gcagctggtc tgtctcggca tgggccaaca tctttacctt
     6001 gccctcgacg ctgccgagcg cgcgagcccg cccgttcgca atgaacggaa aaacgcccct
     6061 cttgtaggga attctcgcct cgcgcagctc gtcttcactc ttgccgacgc tggcaatctc
     6121 cggatgcgtg taaacgatgc ccggaatagc gtcgtagttc acttcgccgt agccggtaac
     6181 gatgtgctcc acgcacgcca ccgcctcttc ggacgcctta tgtgccagca tcggccctcg
     6241 aatcacgtcg ccgacggcgt agacgccctc gacgtaggtc ccgaagtgtt cgttcaccgg
     6301 gatacgtcct cgcggatcga gctcgattcc aatcgactcc agcccgagcc cttcggtgtt
     6361 cggggtgcgt ccgaccgcga ccagcactcg atcgcaacga agtgattctt gccgcgcaat
     6421 ctcgactgtc accccgccgc cctccacgcg cgcgcccgtg acctggcagc cgagtcgaaa
     6481 cttgagcccc tgcttttcga agatcttctc ggcttcctcg gcgatctcgg tgtccatccc
     6541 aggcaggatg cgctccatgt actcgagcac cgtgaccttg gaacccaagc gagaccacac
     6601 cgagccgagc tcgaggccga tatagccggc tccgatcacg accaagtgtt ggggcacttc
     6661 gttgtaagag agtgcgtcgg tgctgtcgcc gatccacttg ccgtcgtgct ccagcccttt
     6721 gagtcgagtc gggcggctgc cggtggccaa aatcacgcgc ggcgcgacga gttcctggag
     6781 ctcttcaccc tcgacgcgca cccggttggg cgctaccagc ttgccgtgcc cgaagtagcg
     6841 ggtgatatgg tgtttcttga acaggtgggc gacaccctgc gtaagcgccg agaccactcc
     6901 gctcttcttc ttgagcatgc ggtgcagatc caactcgacg ccttgcactc gcacgccaaa
     6961 ctcttcgagg ccatgtttcg cttcgtggta gcgctagcta gcctccagca gggccttact
     7021 cgggatacag ccgactcgt
//
LOCUS       DASTKU010000862         4031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_614301, whole genome shotgun sequence.
ACCESSION   DASTKU010000862 DASTKU010000000
VERSION     DASTKU010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614301"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFQ61_30370"
     CDS             complement(<1..364)
                     /locus_tag="VFQ61_30370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958851.1"
                     /translation="MSTRRNGAMARLLPGLILVTLSACERESLDTRPEQVVEEFVQRM
                     QRVHGDPKPARLAYDLLWSEARRNLAERAKRASAVTGRKMGPEEMLAPSRFSLKFVPK
                     RYTSVVDGEWALVTVSSEG"
     gene            complement(378..2111)
                     /locus_tag="VFQ61_30375"
     CDS             complement(378..2111)
                     /locus_tag="VFQ61_30375"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HET9958852.1"
                     /translation="MHRSFRFAALAALLVSAAASPASAFNGFFASKKEGGAKSYTTQV
                     VVMKKAGASVVSVMPDYEGPLEGFALVMLVPEDVTVDGVTTLKREFVDRVDALSAPRF
                     HEFWEQDPCDTTPAQQEWERSLKVEGGGFLGGGPVNQGKKVAKELFLDVQAKQKEGEY
                     KFTVLDAKQDAMGWLSSHGYKSPNGAGAAIQPYVEKGMKVLVAEVDPKRIELVGGDRA
                     QLSPVRYFTTKPFDTIVAKPGLLNSPGKQELVLYVIDPEARYEAKNYKTMFPPTNVAV
                     DFAVKERMGEFYAALHDIILEKNPKTFLSEYAWPADGCGQPCATEPLLIHELLSLGAD
                     VFEKSVPEAERNPKPPALTKEEKDAQQELVKELKPKERKERLKTMEEERKTVLARKGL
                     LERNKYVVSRLHYRYDAASLPEDPTIGPGAGAEGGVALPNGQKMEVSSEVKTTPHNRL
                     QIRYNNFHPWVPVIKCQNPERGKWGKSPPDYRGLRKTWIAEDLSRRNRKQIKPAEVVL
                     TSIPALGLGAAQASNATADAGADAGTAAPAKEKSGCSYVAAPASGSALGFVSVALGAL
                     FGVARRRRRGQ"
     gene            2232..2864
                     /locus_tag="VFQ61_30380"
     CDS             2232..2864
                     /locus_tag="VFQ61_30380"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HET9958853.1"
                     /translation="MEKSRLPKAMTGLSLDRERLRQVLTGRELPVAERCAAVSAVLIE
                     RDTELELLVIRRAERPGDPWSGHMALPGGHREPQDRDLLHTAIRETWEEVGLDLSTAD
                     TLGTLDDLAPHRRGNLLVRPYVFALDHVPTLGVSAEVAHWLWAPLSVLASGSADIEHE
                     VTFSQGTAKFPGFRVGEHVIWGLTYEIVKSLLKQLAGPSTLPSSAILPPR"
     gene            2866..3690
                     /locus_tag="VFQ61_30385"
     CDS             2866..3690
                     /locus_tag="VFQ61_30385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958854.1"
                     /translation="MNALQALSEPAPGGVCRVCEQQLPAGSLACSQCGAVYGEANRCP
                     HCRAIAGIEPDRPAGRCKVCGGPRIALKDPRTLRSGRETHLLRQAEVLQRKRRLSLLF
                     TSLLAASGAFALLATLFVIFIADPGALLATIGFALAAVVGGLAWPLARDARSARRERA
                     RLLEQARLIVASDVIASHAQPVTPLDLASYLETTQEDAELLLAELDLGNMFRARVSVA
                     GEIHHAELSATPAATWLAEDVPVAAPDAQFSDAGPPTATAEAMAFNPALPRPKGDG"
     gene            3780..>4031
                     /locus_tag="VFQ61_30390"
     CDS             3780..>4031
                     /locus_tag="VFQ61_30390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958855.1"
                     /translation="MLRAFIAAKSNLQPTPQAALSSVLRELGVQDWLVLLYLVGLNIG
                     VLQGQRGPGFTTNLARTLGLLCFSIVGLWLCRSGLLRDGW"
BASE COUNT          675 a         1362 c         1216 g          778 t
ORIGIN      
        1 ggccttcgga gctcacggtc accagcgccc actctccatc aactacgctc gtatagcgct
       61 tgggcacgaa ctttagagag aaacgcgagg gggcgagcat ctcttcgggc cccatcttgc
      121 gtcctgtcac cgcgctcgcg cgcttggcac gctccgccaa attgcgacgt gcttcggacc
      181 acagcaaatc gtaagccagt cgcgcgggct tggggtcccc gtgcacacgc tgcatccgct
      241 gcacgaactc ctccacgact tgctccggac gcgtgtcgag cgactcccgc tcacacgccg
      301 atagcgtgac caggattagg cccggcagga gccgggccat tgcgccattg cgacgagtac
      361 tcactttcgg gaagtgctca ctgcccccgg cgacgccgac gcgcaacgcc aaacagcgcc
      421 ccgagcgcca cgctcacgaa accgagcgcc gatccgctgg cgggggcggc cacgtaactg
      481 catccgctct tctctttggc cggggccgcc gtacccgcgt ctgcgcccgc gtccgcggtg
      541 gcattcgagg cctgcgcggc gccgagcccc agtgcgggaa tcgaggtcaa caccacctcg
      601 gcgggtttga tctgcttacg gttgcgacgc gacaggtctt cggcaatcca tgttttgcgc
      661 agcccgcgat aatccggcgg cgacttaccc cacttgccgc gctcggggtt ctgacacttg
      721 atcaccggca cccaaggatg gaagttgttg taacgaatct gcagccgatt gtgcggagtc
      781 gtcttcacct cgctggagac ctccatcttc tgcccgttcg gcaaggccac accgccttcg
      841 gcgcccgcgc caggaccgat cgtcggatcc tctggcagtg acgccgcgtc gtagcggtaa
      901 tgcagtcggg acacgacgta cttgttgcgc tcgagcagcc ctttgcgcgc cagcaccgtc
      961 ttgcgctcct cctccatcgt cttcagccgt tctttgcgct ccttcggctt tagttccttt
     1021 actagctcct gctgcgcgtc tttctcttct ttcgtcaacg caggaggctt cgggttgcgc
     1081 tcggcctcgg gcacgctctt ctcgaacacg tcagccccca aactcaagag ctcgtgaatc
     1141 agcaacggct ccgtggcgca cggttgacca cagccatcgg cgggccaggc gtactccgag
     1201 aggaaggtct tcggattctt ttccaggatg atgtcgtgca gcgcagcgta aaactcgccc
     1261 atgcgctcct tgaccgcgaa atccaccgcg acgttggtcg gcgggaacat cgtcttgtag
     1321 ttcttggcct cgtagcgcgc ctcggggtcg atcacgtaaa ggaccaactc ctgcttgcca
     1381 ggagaattga gcaggcccgg cttggccacg atcgtatcga aaggcttggt cgtgaagtac
     1441 ctcaccggcg agagctgggc gcgatctccg ccgaccagct cgatgcgctt cggatcgacc
     1501 tcggccacca ataccttcat gcccttttcg acgtaaggtt gaatcgcggc acccgcgcca
     1561 ttcggcgact tgtagccgtg gctagagagc cagcccatgg catcctgctt ggcatcgagc
     1621 acggtgaact tgtattcacc ctctttttgc ttcgcctgca catccaggaa cagctccttg
     1681 gccaccttct tgccctggtt cactgggccg ccacccaaga atccgccacc ttcgactttc
     1741 aatgagcgct cccactcctg ctgcgccggg gtggtatcac acggatcttg ctcccagaac
     1801 tcgtggaagc gaggcgcgct caaggcgtcc acccgatcca cgaactctcg cttcaacgtg
     1861 gtgacgccgt ccaccgtcac atcttccggc acgagcatca cgagcgcgaa tccctcgagc
     1921 ggcccctcgt agtctggcat cacgctgacc acactcgctc ccgctttctt catcaccacg
     1981 acctgcgtcg tgtaggactt ggccccgccc tctttcttgc tggcaaagaa accattgaac
     2041 gcggatgccg gactggcggc ggccgagacg agcaacgcgg ccaacgctgc gaagcggaaa
     2101 gatctgtgca tgggggcgga agctagacac acttttggct gctgaacagc cccgaaggca
     2161 gcttgctgtt agagctcggg ccgggacgcg ggcgcgggcc cggcttgagt catggtcgag
     2221 ttgaaagttc cgtggaaaaa tcccgtctac ccaaggcgat gactggcctt tctctagacc
     2281 gcgagcggct gcgccaggtt ctgactgggc gtgaattgcc cgtcgcggag cgctgtgcgg
     2341 cggtctcagc cgtgctgatc gagagagaca ccgaactcga gctactggtc atccgccggg
     2401 ccgaacgccc cggagatcca tggtcggggc acatggcttt gcccggcggg catcgcgaac
     2461 cccaggaccg tgatctgctg cacaccgcga ttcgcgaaac ctgggaagag gtgggtttgg
     2521 acctgagcac ggccgacacg ctcgggacgc tggacgatct ggctccccac cggcgaggca
     2581 acctgctggt tcgaccctac gtgttcgcgc tcgatcacgt tcccacgttg ggtgtcagcg
     2641 cggaagttgc acattggttg tgggcgcccc tctcggtgtt ggcctccggc agcgcggaca
     2701 tcgagcacga ggtgacgttc tcgcaaggaa ccgccaaatt ccccgggttc cgggtcggcg
     2761 aacacgtgat ttgggggttg acgtacgaaa tcgtcaagag cctcttgaag caactggcgg
     2821 gcccttcgac cctaccctcg tctgccatcc tgccgcccag gtaacatgaa cgcactgcaa
     2881 gctttatccg aaccagcgcc aggcggagtt tgccgagtgt gtgagcagca gttgccggcg
     2941 ggctccctgg cttgcagcca gtgcggtgcg gtctacggtg aggccaatcg ctgcccgcac
     3001 tgccgcgcca tcgcgggcat cgagccggat cgtccggcgg ggcgctgcaa ggtgtgcggc
     3061 ggcccgcgca tcgcgctgaa ggaccctcga acactgcgct cgggccgcga aacccatctc
     3121 ttgcgtcagg ccgaagtgct ccagcgtaag cgtcgcctga gcttgctctt cacgagttta
     3181 ctagccgcga gcggtgcgtt cgcgctactc gccacgctgt tcgtgatctt catcgcggac
     3241 cccggtgcgc tgctcgcaac cattggattt gcgctggcgg ccgtcgtcgg cgggttggct
     3301 tggccgctag ctcgagatgc ccgcagcgcg aggagagaac gcgctcgcct tctcgaacaa
     3361 gcgcgtctga tcgtcgccag cgatgtgatt gcgagccacg cccagccggt gacgcctctt
     3421 gatctcgcga gttacctgga gaccacacaa gaagacgcag agctgctctt ggccgagctc
     3481 gatctcggca acatgttccg ggcccgagtg agcgtcgcag gtgagatcca tcatgctgaa
     3541 ctctccgcga cgcccgcggc cacttggctc gcggaagatg tgcccgtcgc cgcgccggac
     3601 gcccaattct ccgacgccgg gccgcccacg gccaccgccg aagcaatggc gttcaaccca
     3661 gcgttgccgc ggccaaaagg cgacggttaa cccgcagatg cccgcggaaa gcggcgggcg
     3721 tcaccgggct ttcgcttgcc ggccctgccc accgaagcta caagcgccgc gtttcgcctg
     3781 tgctccgcgc attcatcgcc gccaaatcca acctccagcc taccccacag gcggcgctct
     3841 cctccgttct acgcgagctc ggagtacagg actggttggt gctgctctac ctggtgggct
     3901 tgaatatcgg agtgttgcag gggcagcgtg gccctggctt cacgaccaac ctggcacgca
     3961 cgctcgggtt gctctgcttc tcgatcgtag gcctgtggct gtgtcggtcg ggcttactgc
     4021 gcgacggctg g
//
LOCUS       DASTKU010000863         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1956317, whole genome shotgun sequence.
ACCESSION   DASTKU010000863 DASTKU010000000
VERSION     DASTKU010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956317"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1183..2928
                     /locus_tag="VFQ61_30395"
     CDS             1183..2928
                     /locus_tag="VFQ61_30395"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HET9958856.1"
                     /translation="MASPAFVIGLDFGTDSVRSAVVDAYSGEVVGTAVEHYPRWRAGL
                     YCDQNRNQFRQHPLDYLESMQASVRAALGQAGDAVARCVRGLAVDTTGSTPVLADESG
                     QPLSLQPRFSENPNAMFVLWKDHTAVAEAEKINRIARSWGGADFTKYVGGVYSSEWFW
                     AKALHILETDVEVASSAATILEHCDWVPAMLTGTALHRIKRSRCAAGHKALWHAEFGG
                     YPSDSFLSLLHPRFPALKQTFGCETFTADTPVGPLSEVWAARLGLPRDTLVSVGAFDA
                     HMGAVGAGIAPHRLVKVMGTSTCDIVLGPRAAQSERVVSGICGQVDGSVLPGLIGYEA
                     GQSAFGDVYAWFKNLLAWPLETVLPKLNAAGASQLARAVADAILPALEESAAALPWEE
                     APLEEAPIALDWLNGRRTPDADQRLTGAISGLRLGSDAARIYRSLVEATAYGSRAIVE
                     RFRAQGVRIDSAIAMGGVASKSPFAMQTVADVMNLEVSVVAGDQPVALGAAMFAAAAA
                     GIHPSVEAAQRAMSCGVERIYRPNPARGLHYDEGYKRYRVLGAFVERELPRRFEDTSG
                     SASLRADRSHLQGFS"
     gene            2925..>3911
                     /locus_tag="VFQ61_30400"
     CDS             2925..>3911
                     /locus_tag="VFQ61_30400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HET9958857.1"
                     /translation="MSSSKSAEIWFLTGSQGLYGAATLDSVAQHAREIASYLDASGRL
                     PARVVWKPTLTSADAILETCLEANSNPDCVGLITWMHTFSPSKMWVWGLTRLAKPMAH
                     LHTQFNRELPWDRIDMDFMNLNQSAHGDREFGFLGARLRMDRKIVVGHWRDPEVIAEL
                     EGWSRAATAYSESQCLKIARFGGMNMREVAVTGGDRLEAQIRFGWSTDGYAVGDLVQR
                     LNEVSDAEIEDVLEQYEATYSLAPALRRGGAQREGLRDAARQEVGIKSFLDAEGCRAF
                     TTTFEDLHGLNQLPGLACQRLMAMGYGFGAEGDWKTAALVRLLKVLSAGRGGG"
BASE COUNT          697 a         1124 c         1303 g          787 t
ORIGIN      
        1 taggtgtgag cagacatatt taacccgacg cctttggcct gactccttga acccgatgcg
       61 cgcctcgtag ttcaaagccg gcaggtcctc tcgttctcga catcgacgcc caagctcaac
      121 cgagctcgct ccggcctggc cttcgttcgt ggctggttcc atggagattc gcaacgcccg
      181 gcgacttccc cgcaggggga cgggcgacgc ctcgcctttg cgtcaccggg gcgggtgcaa
      241 tcgcgccgcc aatcgcgggc gttactggtg cctcgtatcc aggtgcaccc tattgatgat
      301 gagtcccgcc tcgagcggtc acacaagcag gggtcgagag cgcgcccggt cgagttgaat
      361 gtctgctcca ggtctcgacc gtaggtgcaa tcgcgtggct ccgccccgat ctagaaaccg
      421 cggaatttag cgagttgagt ggcgcttcga aaaatcatca ccacgcatgc atcttgaacg
      481 acagatcctg gtgcagagca ccactgcact cgatcagagt cccgtgggct ctacccacac
      541 tcaccctcac actcgtcgac gtcagggatg gcgattatcg cgaccccgct atttagttta
      601 cttttaccga ggcaagcgtg tagccagtca tctagttcat gcgaacagtt atttagttcg
      661 atcgtgcggc tcgaatgccg cgggccgctc ggtggcagtg ccgctcggtg gcagtgggct
      721 caccagcagt ctgtgcctca ggccagcagt ggatttacga gcagtgaaat gagaatccga
      781 gtgtggcgcc gggttttccg agtgtgtccg gtgctcaggg cacctggatc gcgcgacggt
      841 cgcacggatc gcgctgcttg acagctagaa ccaaattgct catggtggcc gcctctctgt
      901 aggtggtttc tcgctcaaga gaacgccgct cgcgcgagat ttggattttg ctcgggtgag
      961 tgcggtagaa ctcgcttgat ggattgaacc cccttggtcg caagggcgct ggattgaaga
     1021 atgagcactg atgcgccgcg aaacggtcgt ggcaaggctc gggatcgtgg tgctgacgtg
     1081 ccgagataga tcgctggagg gaatcagtgt tgcggtgttt gcgcggcttt gttcgaatcg
     1141 atggcgatgg tgagcccctc ggcaccgccg cggcggtgcg caatggctag tccggcattc
     1201 gtgattggct tggattttgg cacggactcg gtgcgcagcg cggtggtgga tgcatactcc
     1261 ggtgaagtcg tagggaccgc cgtcgagcat tatccacgct ggcgtgcggg tctgtattgc
     1321 gatcagaacc ggaatcagtt ccggcaacat ccgctcgact acctggaaag catgcaggcc
     1381 tccgtccggg cggcattggg gcaggcgggt gacgccgttg ctcgctgcgt aaggggtcta
     1441 gcggtcgata cgacgggctc gacgccggtt ctggcggacg aatcagggca accgctctca
     1501 cttcagccac gcttctcgga aaatccaaat gccatgttcg tgttgtggaa agaccacacg
     1561 gccgtggccg aggcggagaa gatcaaccgt atcgcgcgat cgtggggtgg cgcggacttc
     1621 acgaagtacg tcgggggcgt ctattcgtcc gagtggttct gggcgaaagc actgcacatc
     1681 ctggagaccg acgtcgaggt cgcctcttct gcggcgacga tcctggaaca ctgcgactgg
     1741 gttcccgcga tgctgacggg cacggcgctt caccgaatca aacgcagccg ctgcgccgct
     1801 gggcacaaag cgctttggca tgcggagttc ggaggctatc cgtctgattc cttcctgtcg
     1861 ctgttgcatc cgaggtttcc tgcgctgaag cagactttcg gttgcgagac gttcaccgcg
     1921 gatactccgg tgggcccgct atcggaggtg tgggcggcaa ggctgggcct gcctcgtgac
     1981 accctagtta gcgtgggtgc attcgacgcg catatgggtg ccgttggtgc cggcattgct
     2041 ccacatcgtc tcgtcaaagt aatgggaacg agcacgtgcg atatcgtgct cggaccgcga
     2101 gcggctcagt ccgaacgggt cgtgtccggc atttgcggtc aggtcgacgg ctcggtctta
     2161 cccgggctca ttggatatga agcggggcag tctgcttttg gcgacgttta cgcgtggttc
     2221 aagaacttgc tggcgtggcc gctcgagacg gtgttgccga agctaaatgc cgcgggtgct
     2281 tcgcagttgg cacgagcggt tgcggacgcc attctgcctg cgctcgagga gagcgcagcc
     2341 gctctaccgt gggaagaagc gcccttggaa gaggcgccga tcgcgctcga ctggttgaat
     2401 gggcgccgca cgcccgatgc tgaccaacgt ctcacgggcg cgatatccgg gcttcggctg
     2461 ggcagtgacg ccgcgcgcat ctatcgcagt ctggtcgagg cgaccgcgta tggctcgcgg
     2521 gccatcgtgg agcgttttcg ggcacagggg gttcggattg atagcgcgat cgccatgggg
     2581 ggcgtggcga gcaagagccc gttcgccatg caaacggtgg cggacgtgat gaacctggaa
     2641 gttagcgtgg tggccggtga ccaaccagta gcgcttggcg cggcgatgtt tgcagcggcg
     2701 gcggctggaa tccaccccag cgtggaagcg gcgcagcggg ccatgtcgtg cggcgtcgag
     2761 cggatataca gacctaatcc tgcgcgcggt ctccactacg atgagggcta caaacgctac
     2821 cgcgtgctcg gcgccttcgt ggagcgggag ttgccccgaa gattcgaaga cacgagcggt
     2881 tcggccagtc tgcgggcgga ccgttcccac ctgcaaggtt tctcatgagc agttctaagt
     2941 cggcagaaat ttggtttttg acgggtagcc aagggctcta cggggcggcc actctcgata
     3001 gcgttgctca acacgcgcgt gagattgcca gctacttgga cgcctcgggc aggctaccgg
     3061 cgcgagtagt gtggaaaccc acgctcacga gcgcggacgc gatcctcgag acgtgtctcg
     3121 aggcaaactc gaatcccgat tgtgtcgggt tgattacctg gatgcacacc ttttcaccct
     3181 cgaaaatgtg ggtatggggg ttaacgcggc tcgccaagcc gatggcgcat ctgcacaccc
     3241 agttcaaccg ggagctgccg tgggatcgca tcgacatgga tttcatgaac ctgaaccagt
     3301 cggcgcacgg tgaccgtgag ttcgggttcc tcggcgcgcg gctccgcatg gatcgcaaaa
     3361 tcgtagtcgg ccactggcgc gatccggaag taatcgcaga gctcgagggt tggtcacgcg
     3421 cggcgacagc gtattccgag tcgcaatgcc tgaagattgc gcgcttcggc ggaatgaaca
     3481 tgcgcgaggt cgccgtaacg ggcggcgacc gcctggaggc gcaaattcgc ttcggctggt
     3541 ctacggacgg ctacgcggtg ggggatctgg tgcagcgctt gaacgaggtg agcgacgccg
     3601 agatcgaaga cgttttggaa cagtacgaag ccacctactc gctcgccccc gctctgcgtc
     3661 gtggcggcgc tcaacgcgaa ggcttgcgcg acgcagctcg acaagaggtt ggcatcaagt
     3721 ccttcctaga cgccgagggt tgccgcgcct tcaccaccac gttcgaagat cttcacgggc
     3781 tcaaccagct ccctgggctt gcctgccaac gcttgatggc gatgggctat ggcttcggcg
     3841 cggagggaga ctggaaaaca gccgccctag tccgactcct gaaggtactg tcggccggac
     3901 gcggcggtgg c
//
LOCUS       DASTKU010000864        13615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_390830, whole genome shotgun sequence.
ACCESSION   DASTKU010000864 DASTKU010000000
VERSION     DASTKU010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13615
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390830"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFQ61_30405"
     CDS             <1..372
                     /locus_tag="VFQ61_30405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9958858.1"
                     /translation="FEPGRGTRLSTWLGLLATHTAYDFLRSVRNAPKLDLSSAEKMSA
                     DAPDPVEATLQRERAELVARAISELSDKDREFVELYYGQGLSPEEVAERMQISVKTVY
                     TKKHKLQHRLQGLLSDERLAA"
     gene            438..2876
                     /locus_tag="VFQ61_30410"
     CDS             438..2876
                     /locus_tag="VFQ61_30410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958859.1"
                     /translation="MLFRAASSQWATAAVCISALALLRCGGRLPATQRAQARAESGES
                     PIERLVVDRRPALTLVARGGDPHSAVAFVATHARGSVASAALAGLLAARMQGGRDDLP
                     VLARAHGLGFVISAQVDRAVDAQRFVRRLREVLAQPVQADEPALARARSELDRLRSAA
                     FSGSGEAAAAACSGEFGHFQTAAIWDLGHSAGLAALEAARRAVFAPKASAFAALGSSE
                     VLDAAGRALEDSPAWPDAEAEQEKAALRDEFYVDAVEGRPRLIVALRVGDADAAVTAG
                     TELGSADSELLQHLATFTPKWKLERSLAISRVRGACLRLDLSLPDGTPAPAIEEVAKV
                     AAITNAIAERALTHGVPGALDESVMRPTDALRACELAAWRGLSGSNVELPPRRVVAYL
                     GERAMSKAGTPELEAAFARYRSELGRPELELVSRAEPGQGEFWMLVGSPCGTGPETNA
                     TAGAGALAMLTLARRATEVGVRLEAWVTPDGVGLLAHSPRLDSDESAADHAARVARAL
                     GRAWATPITGPETAQPRASLLDALGGRPYPALARALEGLAPDHPSWLEPRGTFAQVAE
                     LSSEEVERARRQLLVGPLRVAVLASRGEAQVSGLRGELGSWLLPWRGRAASCPDSSAV
                     SPQGGEVRLMPMASSAGLGPEPAYVGVAWPDSGNGARSAPELIAWLLNRPGGPLERAS
                     RSLQPSVGARAHVYGGKRRPAVLVEIRALPEQLTQAVGSVRAALDRVAQSGFNADELR
                     TAQLDISRAEDAARGDPRKRLVDLWRGETGNDSGLQGRSHAGELSPNQLVTGRLAASH
                     HWVVLPSPIQAAGP"
     gene            3018..4568
                     /gene="guaA"
                     /locus_tag="VFQ61_30415"
     CDS             3018..4568
                     /gene="guaA"
                     /locus_tag="VFQ61_30415"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET9958860.1"
                     /translation="MAKRQSVLILDYGSQYTQLIARRVRESHVYSEIHPCTLAFEAIQ
                     AMAPSAIIMSGGPQSVYDATAPKSDPRIFDLGVPVLGICYGEQLMAQQLGGKVEPSPE
                     REYGPARLRIDESVGILGCFGAGEEIAVWMSHGDRLTQAPAGFRVIGRSENAPFAAIA
                     NPERRLYGIQFHPEVAHTPRGSELLRAFLFELAGLEPSWTPGSFVEEAVADIAARVGP
                     DERVICGLSGGVDSSVAAVLCQRALGERLVCIFVDNGLLREGEYENVVHTMRQSFHLN
                     LVPVDASREFLDALAGVTDPEQKRKIIGRVFIEVFEQHAARVENAKYLVQGTLYPDVI
                     ESVSVRGPSAVIKSHHNVGGLPERMKLKLIEPLRELFKDEVRKVGEAMGMPNDVLYRQ
                     PFPGPGLSVRCLGDLTPQRLDVLRKADRIVDEEIRAAQLYTSLWQSFAVLLPVRSVGV
                     MGDDRTYEETCVVRAVHSSDGMTADWARLPHDVLARISSRITNEVRGINRVVYDISSK
                     PPATIEWE"
     gene            4565..5020
                     /locus_tag="VFQ61_30420"
     CDS             4565..5020
                     /locus_tag="VFQ61_30420"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9958861.1"
                     /translation="MTGAQVQTATSEPKSDSAWLESTRARTGALVRRARALGCFFALV
                     GGQVPPKAVVAAVSLATLTSVGCRAGVGRAAVSLSLAREKSTPKDASVFIDEEFIGPL
                     TFVAAHGVRLPVGEHRITIEKAGYFPWDRLVVADRQPIKLDVQLEPIPD"
     gene            5115..5188
                     /locus_tag="VFQ61_30425"
     tRNA            5115..5188
                     /locus_tag="VFQ61_30425"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5149..5151,aa:Pro,seq:tgg)
     gene            5518..6318
                     /locus_tag="VFQ61_30430"
     CDS             5518..6318
                     /locus_tag="VFQ61_30430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HET9958862.1"
                     /translation="MRRSLLWLSALLSLLAACEEKPVPKPDAAPTATAAPLPTPAPAP
                     TPAPTPSPEAAAPKPKRRLEDCPKGAQFEFSDKAIELEVRRKLPKPEGPITVADLRRL
                     RSLNLSQVKLSDLDVCLFTNLRGLKELFLGPGDFDDLSPIATSTQLESLRASINQVKD
                     LKPLEKMSNLDRLDLGRTQVSDLKPLASLKKLSELQLDGTNVEDLTPLANLTELTTLS
                     LKNTKVKDASPLKGLKKLKFLYVGGSPLDADPMSVAPVRANGTKVLAE"
     gene            complement(6398..7438)
                     /locus_tag="VFQ61_30435"
     CDS             complement(6398..7438)
                     /locus_tag="VFQ61_30435"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HET9958863.1"
                     /translation="MTITMVKKQFADGSTCQKCVQAEELLRRRGLWARIDRVVWAVET
                     DANSEGMRLAATHGVSLAPFFVVEGGAQPRIHTSVLKLINEELSPASSNQPVEGASRV
                     TAALPDVEQLERALDGRAPEVIVAQALELFGADLAIAFSGAEDVALIDMAVRSGHPFR
                     VFSLDTGRLHPQTYQFLERVRDHFGIQIEAYYPDVVGVEELVRRKGLFSFYRDGHEEC
                     CHVRKVLPLGRALNGRRAWMTGQRRDQSPTRSDVKVVAPDRQRTFEGKPLWKWNPLAG
                     FTLDQVWAYIRERELPYNTLHDQGFISIGCEPCTRAVRPGEHERAGRWWWEESTQREC
                     GLHVAKPGAAST"
     gene            7563..7718
                     /locus_tag="VFQ61_30440"
     CDS             7563..7718
                     /locus_tag="VFQ61_30440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958864.1"
                     /translation="MANRAIWNVRKRHEWLSTPLKGKRLNKRKRVRIGLEQARERQKG
                     SEASSTS"
     gene            8998..10770
                     /locus_tag="VFQ61_30445"
     CDS             8998..10770
                     /locus_tag="VFQ61_30445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958865.1"
                     /translation="MTLLSAVAESLSSWSSAPSEFWNSWTFFQKDTWEPFTKGFRILI
                     ALLGVLALLYEIRARRMGERIRERTRRRLAWLFTILAFGVYFDFGNPNTRYSEYYHRH
                     EFYHYYLGSKYFKEIGYTRLYECTLIAEVENGRLAQVKDRDFRDLRVNLIKEVKDSYI
                     LSDPDQCKKYFTPDRWASFKREIDWFYRSAAGSYWEGMQRDHGYNPPPVWTMTGKFFG
                     SFHEADDGYFKLLAGIDVLLHLGIVLLFKWAFGWRVMAVATVFWGANAPADFYWTGGA
                     FLRQDWLFLLVAALCCARKRWFFAAGFALTWSALLRIFPGIFVVGWAIIMGLYVLQAI
                     RRGPPQIAGDKATGLLRYIHPDHRRVILGCVVAVATLVPASIAVCGVDSYKEFYQHTL
                     KTHQNTPLTNHMGLESMVVHNWEGRMRFGRDDNMSDPFEGWKKGRLERFRQMKPVFIA
                     IVLSLFAWTVWALRRTRLLWVGTALSLPLVMSLTNLTCYYYSMFIVGAALVAVRPQLG
                     APYLAASAIGKMLQWSPIGFYWVDDRWVAESWLYFILCLILLAGYSRPFSMERLKAWW
                     DGKREPRSKSQSSSPPLAPAADSG"
     gene            complement(10939..11805)
                     /locus_tag="VFQ61_30450"
     CDS             complement(10939..11805)
                     /locus_tag="VFQ61_30450"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET9958866.1"
                     /translation="MNAPANEPRPVANYHCEIGENPLWNPDDACLYWVDIATGRLFRA
                     RHADGAHECFWQGDAIGGFTLQADGSLLLFEADRIARLDLRTGQRRVLIEGIDPDMAR
                     FNDVIADPEGRVFAGTFGNTQESGGVYRIDPDGSYRCLWKGTQIANGMGFTRDLKRFF
                     WTCSTTARIFEFDYDRVTGELSNRRLLYQAQPSEGSPDGLTVDSEDTIWTARWGGHQV
                     LRLDLQGRVVSRLELPVPKVSSVTFGGPQLDTLYITTAGGDSSSTAADGTLYRWHAPV
                     RGQADFRSRVLL"
     gene            complement(11851..12552)
                     /locus_tag="VFQ61_30455"
     CDS             complement(11851..12552)
                     /locus_tag="VFQ61_30455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958867.1"
                     /translation="MNTSGAGSLDRRSMDKLLSEGISVLEAAFAYVRQHPEEVIRVLR
                     NAAGLRFGVPMAALRWLAGKASGKKAPKDVEISAVPPGIRVGGSFELMGTPLRALADV
                     YIDSVTITAEELSFGVRLKNVSLRVTDDKVESPLAALLRSGALDLSKPGNLVAYMPKR
                     PAILTEAKDDRIRIDLFRHPKLKSDPKLKRLLAAIVPLVTVGAIETEGDHLDVRLRAF
                     PEGVSQAVERLRKLF"
     gene            12830..>13615
                     /locus_tag="VFQ61_30460"
     CDS             12830..>13615
                     /locus_tag="VFQ61_30460"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9958868.1"
                     /translation="MSHVEPAEVHATLGRHLIASGHPLVLDLARSQGTFLYDALGGTE
                     YLDFAGFYASNALGFRHPSLETETARQKLLESAAIKVGNPEFYTVAFAEFVAAVERIA
                     APSTHPHYFFVEGGALAVENAMKAAMDWKVRHNLARGLGEIGTEILHFERAFHGRAGY
                     TLSVTNTDPAKTQYFPKFDWPRVPSPALRFPRSAEHDAATLAAEQACLSQIESAFEAR
                     PHRIAAILIEPIQGEGGDNHFRPEFLQALRRIADQREALLIFDE"
BASE COUNT         2395 a         4220 c         4404 g         2596 t
ORIGIN      
        1 ttcgagccgg gtcgcggtac tcgtctcagt acctggcttg gcttgcttgc cacccatacc
       61 gcgtacgact tcctgcgctc ggttcggaac gcgcccaaac tagacctgag ctcggcggag
      121 aagatgtccg ccgacgctcc cgatccggtg gaagccacgc ttcagcgtga gcgcgcagaa
      181 ctcgtggccc gcgcgatcag cgagctgtcc gacaaggatc gtgaattcgt ggaactttac
      241 tacggccagg gactttcacc cgaggaagtg gccgaacgca tgcagatcag cgtgaagact
      301 gtttacacga agaagcataa acttcagcac cggcttcaag gcctgctgtc ggatgaacgc
      361 ctggccgcgt gagctccgca ttctccgccg atatttcgga ggtccatcgc gacgaagacg
      421 tgtgctaaac gggcgacgtg ctgtttcgag cagcgagctc gcagtgggcc accgcggcag
      481 tctgcatcag cgcgttggcc ctgctgcgct gtggaggccg cttgccggct actcagcgcg
      541 cgcaggcgag agcagagagc ggggaatcgc cgatcgagcg actggtcgtg gatcgacgcc
      601 cagcgctgac tttggttgca cgcggaggag acccgcactc ggccgtggct ttcgtagcca
      661 ctcacgctcg cggcagcgtg gcgtccgcag ctttggcggg attgctggcg gcccgcatgc
      721 agggcgggcg cgatgacttg cccgtgctgg cgcgcgcgca cgggctcggc ttcgtaatct
      781 ccgcgcaagt ggaccgtgcc gtagacgcgc agcgcttcgt gcgtcgattg cgtgaggtgt
      841 tggcgcaacc ggttcaagcc gacgagcccg ccctcgcccg cgcccgaagt gagctcgatc
      901 gcctgaggtc tgccgccttt tcaggctcag gtgaggccgc ggccgcggcg tgctcgggcg
      961 agttcggtca tttccagacc gcggccattt gggacctcgg tcacagcgcc gggctcgcgg
     1021 cgctcgaggc cgcacgtcgg gcggtcttcg cgcccaaggc ctcggctttt gctgcgctag
     1081 gtagcagtga agttttggat gccgcgggtc gtgctctcga ggactcgccg gcttggccag
     1141 acgccgaggc agaacaggaa aaagctgccc tgcgcgacga gttctatgtc gacgccgtcg
     1201 aggggcgccc gcgcctcatc gtcgctcttc gcgtgggaga tgcagacgcc gccgtcaccg
     1261 cgggcaccga gctcggttcg gcggattccg agctgctcca gcatctcgcc accttcacgc
     1321 ccaagtggaa gctcgagcgg tcgctggcca tttcgcgggt acggggcgcg tgtctgaggc
     1381 tggatttgtc tttgcccgac ggcactccag cgccggcaat cgaagaagtc gcgaaggtcg
     1441 cggccatcac gaacgccatc gctgaacgcg cattgacgca cggcgtaccc ggggctctcg
     1501 acgagagcgt aatgcgcccc accgacgcgc tgcgagcctg tgagctcgcg gcgtggcgcg
     1561 ggctgtccgg aagcaacgtc gaactgcccc cccggcgggt ggtcgcctac ttgggcgaga
     1621 gagcgatgtc caaggcaggg actccggagc tggaggcagc cttcgcgcgc taccgctcgg
     1681 agcttggacg gccggagctc gaactcgtgt ctcgcgccga acccgggcag ggtgagtttt
     1741 ggatgttggt cggaagtccg tgtggaacgg gtcccgagac caacgcgacc gcgggcgcgg
     1801 gcgcgctcgc gatgttgact ctggcgcggc gtgctaccga ggtcggagtt cggttggaag
     1861 cttgggtcac cccggacggg gtgggactct tggcacactc gcctcgtctc gactcggacg
     1921 agtccgctgc cgatcacgcg gcacgcgttg ctcgggcgct ggggcgagct tgggcaacgc
     1981 caattaccgg ccccgaaaca gcccaaccac gagcaagcct gctcgatgca ttgggcggcc
     2041 gcccctatcc tgctttggct cgcgcgttgg aggggctagc gccggatcac ccttcatggt
     2101 tggagcctcg gggtacgttc gctcaggtcg cggagctgtc gagcgaggag gtcgaacgcg
     2161 ctcgacgtca gctgctcgtt ggccctttgc gagtcgcagt gctcgccagt cgaggagaag
     2221 cgcaggtctc gggcttgcgt ggcgagctcg gctcgtggct cttaccttgg cgcggccgag
     2281 ccgcgagttg cccggacagc agcgcggtga gcccgcaagg gggtgaggtc agactcatgc
     2341 cgatggcgag cagcgccggc ttgggtccgg agcccgccta tgtcggcgta gcgtggcccg
     2401 attcggggaa tggcgcacgc tctgctcccg agttgatcgc ctggctgctc aatcgcccgg
     2461 gggggccgct cgaacgtgcg agccgcagcc tccagccgag cgtcggtgcc cgcgcccacg
     2521 tgtacggggg caagcgccgg cccgcagtcc tcgtggagat ccgagcattg ccggagcagc
     2581 ttacgcaggc ggtgggaagc gtccgcgcgg ctcttgaccg cgttgcgcag agcggattca
     2641 acgcggacga gctacgtacg gcccagctcg atatttcgcg agcagaagac gcggcgcggg
     2701 gcgaccctcg gaaacgcctg gtcgatctgt ggcgcggtga gacgggcaac gattcagggc
     2761 tgcagggccg ttcgcacgcc ggcgagctct cgccgaacca gctcgtgacc gggcgactcg
     2821 cggcgagcca tcactgggtc gttctgccct ccccgatcca agccgctggt ccgtaaactg
     2881 ctgaatccgg agcagatccg gttgggctcg cccctcgctt ctggttacga tcagcagatc
     2941 gaccgcgttt cgatcagcaa aagccgcgcg gtggtttgca cgcggcagtt gtcgtgttag
     3001 ctccctcgcg gcgcaggatg gctaaacgac agagcgttct cattctcgac tacggatcac
     3061 agtacacaca gctgatcgct cgccgcgtgc gcgagagcca tgtgtactcg gaaatacatc
     3121 cctgcacttt ggctttcgaa gccattcagg ccatggcgcc gagtgcgatc atcatgagcg
     3181 gtgggccgca gagcgtctac gacgccaccg cgccaaagag cgaccctcgc atcttcgacc
     3241 tgggcgtgcc ggtgctcggg atttgctacg gcgaacagct catggcccag cagctcgggg
     3301 gcaaggtcga accgagtccc gagcgggagt acggccccgc gcggctccgg atcgacgaga
     3361 gcgtggggat cttgggctgc ttcggcgctg gcgaagagat cgcggtttgg atgagccacg
     3421 gtgaccgtct cacccaggcg cccgcaggct ttcgggtcat cggtcgcagc gagaacgccc
     3481 ccttcgccgc gatcgccaac ccggaacgac gcctctacgg aattcagttc catcccgaag
     3541 tcgcgcacac tccgcgcgga tcggagctgt tgcgcgcctt cttgttcgag ctggcggggt
     3601 tagagccgag ctggacgccg ggatccttcg tcgaggaggc cgtcgccgat atcgccgcgc
     3661 gggttggccc cgacgagcga gtgatctgtg gtttgagcgg cggcgtggat tcttcggtgg
     3721 ccgccgtact ctgccagcgt gcgctcgggg aacgactggt ctgtatcttc gtagacaacg
     3781 gcttgctgcg cgagggcgag tacgaaaacg tcgtacacac catgcggcag agcttccacc
     3841 tgaacctcgt gccggtggat gcgagccgcg agttcttgga cgccttggct ggagtcactg
     3901 accccgagca aaagcgaaag atcataggtc gggtgttcat cgaggttttc gagcagcatg
     3961 cggctcgcgt ggagaacgcc aagtacctcg tacagggcac gctctaccca gacgtgatcg
     4021 agagcgtcag cgtgcgtggc ccgagcgccg tcatcaagag ccaccacaat gttggcggtc
     4081 tgcccgaacg catgaagctc aagctgatcg agcctctgcg cgagctattc aaagacgagg
     4141 tgcgcaaggt cggtgaagcc atgggtatgc cgaacgacgt gctgtatcgg cagcccttcc
     4201 ctggtcccgg gctctcggtg cgctgcttgg gtgatctcac gccgcagcgg ctggacgtgt
     4261 tgcggaaagc ggaccgcatc gtggacgaag agattcgcgc cgcgcagctg tatacgagcc
     4321 tttggcaaag ctttgccgtc ctgctgccgg ttcgcagcgt gggcgtgatg ggcgacgatc
     4381 gtacgtacga ggagacctgt gtggttcgcg ccgtgcactc cagcgatgga atgacggcgg
     4441 actgggcacg cctgccgcac gacgtgctcg cgcgcatcag ctcccgcatc acgaacgagg
     4501 tgcggggcat caaccgagtg gtctacgaca tcagctcgaa gccacctgcc actatcgagt
     4561 gggaatgacc ggagcgcagg tccagaccgc aacgtcagag ccgaagtccg actcggcttg
     4621 gctcgaaagc acgagggcgc gtacgggcgc cctcgtgcgg cgcgctcgtg cactcggatg
     4681 cttcttcgcg ctggtcggcg gccaggttcc gcccaaagcg gtcgtcgcgg ccgttagcct
     4741 tgcaacattg acgagcgtcg gctgtcgagc gggcgttggt cgggcggccg tgtccctgag
     4801 cttggcgcga gagaagagta cgcccaaaga cgcgagcgtg ttcatcgacg aggagttcat
     4861 cggcccgctc acgttcgtcg cggctcacgg ggttcgactc ccggttggcg agcatcgaat
     4921 cacaatcgag aaagctggtt actttccctg ggatcgcctc gtggtcgccg atcgacagcc
     4981 cataaaactc gacgtgcagt tggagccaat ccccgactag cgcaccggga tcgctcctcg
     5041 cccgtgaagc cagcaaaatc ctactccgcc tatggcaacg ccgaacaaac cttgctataa
     5101 gcgcggcccc taatcggggt gtacctcaac ctggtagagg gccggctttg ggtgccggct
     5161 gttgcaggtt caaatcctgc caccccgaca aaacacccgc gacgctggcg ccatgctcga
     5221 ttgagatggg agggcgtgcg gcccttggaa aactcgcggt ctgtggaatg cgtcttgccg
     5281 cagcgacgtg ttgttcgcgc agcgacgtgt tgttcgcgca gcgacgtgtt ctttggcgca
     5341 gccacgtgtt cttggcgcac cacggtgtta tgcgactgac ggtatcgcgg ctgctgagaa
     5401 ggagctgcgg tgcggccgtc attttgcgag ccatcgggta tctgtgacgc ttggctcgcg
     5461 gcgcgcggcc gtacagtctt gatcttggca cctgctcgcc tggtagagtg ccgggcgatg
     5521 cgccgaagtc ttctgtggtt gtctgcactg ctgagtctac tggctgcgtg tgaggagaag
     5581 cccgttccga agccggatgc tgctccgacc gccacggccg cgccgctccc tacgcccgct
     5641 cctgcgccca cgcccgcccc aaccccgtcg cctgaagccg cggcgccgaa acccaagcgc
     5701 cgcctggagg attgcccgaa gggagctcag ttcgagtttt ccgacaaggc aatcgagctc
     5761 gaggtgcgtc gcaagttgcc gaaacccgag gggcccatta ccgtcgcgga tttgcgcagg
     5821 ctgcgctccc tgaacctctc gcaagtgaag ctgagcgact tggacgtctg cctgttcact
     5881 aacctccgag gcctgaagga gctctttctc ggccccggtg acttcgacga tctttccccg
     5941 atcgctacct ccacccagct cgaatcgctg cgggcgtcaa tcaatcaggt caaggacctg
     6001 aagccactcg agaagatgtc gaacttggat cggctagatc tcgggcgcac gcaagtgagc
     6061 gatctcaagc cactcgcctc tctgaagaag ctgagcgagc tgcagctcga cggcacgaac
     6121 gtggaagatc tgactcccct cgccaacttg accgagctga ccaccctcag cttgaagaat
     6181 accaaggtca aggatgcgtc tcccctcaag ggcctaaaga agctgaagtt cttgtatgtc
     6241 ggcggctctc cgttggacgc ggatccgatg tcggtcgctc ccgttcgcgc caacggcacg
     6301 aaggttttgg cggagtagcg cgcaacccga attcgtcacg gcagctgcga atgaatgcag
     6361 ctgccgtgag cctgttcggg gcctgccctg ggttcggcta agtagaggcc gcgccaggtt
     6421 tagccacgtg aaggccacat tcgcgctggg tgctctcttc ccaccaccag cgccccgcgc
     6481 gctcatgctc gccagggcga accgcacgag tgcagggttc gcaaccaatc gaaatgaatc
     6541 cctgatcgtg gagcgtgttg tacggtagtt ctcgctcacg aatgtaggcc cacacctgat
     6601 cgagcgtgaa gcccgcgagt gggttccact tccagagagg tttcccttca aacgtacgct
     6661 gtcgatccgg tgcgacgact ttcacgtcgc tgcgggtcgg gctctggtct cgacgttgcc
     6721 ccgtcatcca agcccgacgc ccattcaacg cgcgaccgag cggcagcacc ttgcgcacgt
     6781 ggcagcactc ttcgtggccg tcgcggtaaa agctgaacag cccctttcgg cgcacgagct
     6841 cttcgacccc cacaacgtcc gggtaataag cctcgatctg gatcccgaag tggtcccgca
     6901 cccgctcgag gaattgatag gtttggggat gcaaccggcc cgtgtccaag gagaagaccc
     6961 gaaacggatg cccgctgcgc accgccatgt cgatcagcgc gacatcctcg gcgccgctga
     7021 acgcgatcgc aaggtcagct ccaaaaagct ccagggcttg ggccacgatc acttctggag
     7081 cgcgtccgtc gagagcccgc tcgagttgct ccacgtcagg tagtgctgcg gtcacccggg
     7141 aagccccttc tacgggttgg ttcgaactgg ccggcgagag ttcttcgttg atcagcttga
     7201 gcacacttgt gtggatccgc ggctgcgcac ctccctccac gacgaagaac ggcgcgaggc
     7261 tcacaccatg agtggccgcc aaacgcattc cctcggaatt ggcgtccgtt tccaccgccc
     7321 aaaccacacg atcgatgcgc gcccaaaggc cacggcgccg aagcaactct tccgcctgga
     7381 cgcatttttg gcaggttgag ccatctgcga actgcttttt caccatcgtg atggtcatgg
     7441 gattccgcgt ggaatctggg ggcttggcga gcaaagcgca agccagaagg tcagtcaggt
     7501 cggagcttcg gtgactctgc ccttgcgcga ggatccgaac tcgactactg ggagtgcccc
     7561 caatggcgaa tcgagcaatt tggaacgtgc gcaagcggca tgaatggctt tcgacccctc
     7621 tcaagggcaa gcgcctcaac aagcgcaagc gcgtgcgcat tggtttggag caggcgcgcg
     7681 aacgtcaaaa gggcagcgaa gccagctcga ccagctgagc tctggggcgc ccctagaggg
     7741 cgccgctcta agctcttgga ccgagcgccg gataatgcga gcgccggata ttagagcctg
     7801 ctctcgcctc acgcttcgca cagcggggag ctcgcgatcg tcctgagaac cggtccgagc
     7861 caccgcgaga cttatcgaag ctcgaggctc ggaagcgtag tgtctcggaa gagcagtgtc
     7921 ttgacggctt tagccgtcgg tcttaccgcc cgatggggta ggacatttgt gcacctcaaa
     7981 aaatcgcagg aaaaggcgtc cgtagcgttc gtcgtgagcg gcagctcgct gagagcgtgc
     8041 tacgttgttg cctatcgtgc tgcgaggaga acaactcccg gaatcatccg acgggggagc
     8101 acccctggta acggcacaag gcccggaggc cgcgcgaacc tggtggtatc gacccacgcg
     8161 gttctccctt tcggaagcgc tcgctactcc ccgctgctct tcaatgggca ctctagtgat
     8221 tgagtacgct gcgatagcgc acgtctctcc agaggcttgg ggaaccgagc gtccggcaag
     8281 taccggagga ggtcaaggca tgcagagtag ttgttcccct aacccggagg tttggccaac
     8341 ctagctcggc gtcgatctcc ggtattcaac cggatggatc gatcgtgtcg cctgcttagc
     8401 gagcaggcgg gccggcgggt gaggcctggt gcctcgcaag gcttattcga gagcagcgcg
     8461 ccgttaacgt cgcgctgagg tcgtaaacag cgtgccggca gcggcgagcc gacttcgcgc
     8521 gtggatcgcg cgggcggcgg catcgccagt ttcctggaac cttccggggt ttcggggctc
     8581 gcaacggcgg gccgggtacc aaacgcggtg acccggcccg ttttctattt gggggttggg
     8641 cttccagctc gagtctggag tttcactcgg ttttgagttg ccttcgaggc tttcgttcgc
     8701 agcgccggtc tgggtcgacg ggttgcgcga aacgggagca tcaacgaaga gtgcccatcg
     8761 aacggagtgg ggaccttcaa cggaagggca cttatcgaac ggagcggggc catcgaacaa
     8821 gccaagcagc agttggcttc ggccgaatca tacgctgccc ttgcgaaacg ggccggtcgg
     8881 gatgccgttg ttctgatccg gttttggcct taactcgcgt gaccggttgc ttggggcgcg
     8941 ctgttggttt acgaaccggc agcgccgcgg caaagcggcg ggaggatttc gtcgcgcatg
     9001 acgctgcttt cggccgtagc tgagtcgctg tccagttgga gctcggcgcc tagcgagttc
     9061 tggaattcct ggacgttctt tcaaaaggat acctgggaac ctttcacgaa agggttccgc
     9121 atcctgatcg cgctgctcgg agtgctcgct ctgctctacg aaatccgcgc gcgacgcatg
     9181 ggagagcgga tccgtgaacg tacgcggcgc cgcctcgctt ggctgttcac gatcttggcg
     9241 ttcggggtct acttcgactt cggaaacccc aatactcgat attccgagta ctatcaccgc
     9301 cacgagtttt atcactacta cctcgggtcc aagtacttca aggagatcgg gtatacgcga
     9361 ctctatgagt gcaccctgat cgccgaggtg gagaatggcc gcctcgctca ggtaaaagac
     9421 cgagacttcc gtgatttgcg cgtcaacctg atcaaagagg tcaaagactc gtacattctg
     9481 agcgatcccg accagtgcaa gaagtacttc acaccggatc gctgggcctc gttcaagcgc
     9541 gagatcgact ggttctatcg ctccgcggca gggagctact gggagggtat gcagcgcgat
     9601 cacggctaca acccgccccc cgtttggacg atgacgggca agttcttcgg cagcttccat
     9661 gaggccgatg acgggtattt caagctgctc gcgggcatcg atgtgctgct gcacctcggc
     9721 atcgtgctgc tcttcaaatg ggcgttcggt tggcgcgtga tggccgtggc gaccgtgttc
     9781 tggggggcta acgcgcccgc cgatttctat tggacgggcg gcgctttcct gcggcaagac
     9841 tggctatttt tgctggtcgc cgcgctatgc tgcgcgcgta agcgctggtt ctttgcggcg
     9901 ggctttgccc tcacctggtc cgcgttgctg cgtatcttcc cgggcatctt cgtggtgggc
     9961 tgggccatca tcatgggcct ttacgtgctg caggcgatcc gtcgcgggcc gccgcaaatt
    10021 gcgggcgata aggccaccgg tctgctgcga tacattcatc ccgatcaccg gcgcgtgatc
    10081 ctgggttgcg tggtcgcggt cgccaccttg gtgcccgcca gcatcgccgt ctgcggagtg
    10141 gactcataca aggagttcta tcagcacacc ctgaagaccc atcagaacac gccgctcacg
    10201 aaccatatgg ggctcgagag catggtcgtg cacaactggg agggccgcat gcgcttcgga
    10261 cgggacgaca acatgagcga cccattcgaa ggctggaaga aagggcgcct cgagcgattc
    10321 cgtcagatga agccggtgtt catcgcgatc gtgctctcgc tcttcgcctg gacggtctgg
    10381 gcgctgcgcc gcacgcgctt gctctgggta ggaacggccc tcagcttacc cctggtgatg
    10441 agcctcacca acttgacctg ctactactac tcgatgttca tagtgggcgc ggcgctcgtc
    10501 gcggtgcgac cacagctcgg ggcgccgtac cttgccgcga gcgcaattgg gaagatgttg
    10561 cagtggtcgc cgatcgggtt ctattgggtg gatgaccgtt gggtcgctga gagctggctg
    10621 tacttcatcc tgtgcctgat cttgttggcc gggtactcgc gtccgttcag catggagagg
    10681 ctgaaggcct ggtgggatgg caagcgtgag ccgcggtcga agagccaatc ctcttcgccc
    10741 ccattggctc ccgcggcgga ctctggttag ccgaaacgtg cgtctggggt tctgctgagt
    10801 accgggctga accctagaca tgcacggaca gtgacgagtc gccggaaggc cgggaagccc
    10861 gactttcggt gacgaaaaaa aaacgaggtg gctcagtgag ccgcctcgtt tcgttttgtg
    10921 gggaccgcgc gcgcagcgct agagcaaaac gcgcgatcgg aaatcggcct ggccgcgcac
    10981 cggcgcgtgc cagcggtaga gcgtgccgtc ggcagcggtg ctgctcgagt cccctcccgc
    11041 cgtcgtgatg tagagcgtgt cgagctgcgg cccaccaaat gtcaccgaag aaactttggg
    11101 aaccggcagc tccaagcgcg acacgactcg accttgcagg tccaagcgca gaacctgatg
    11161 gccaccccag cgagcggtcc aaatcgtatc ctcgctgtcg acggtgagcc cgtccggcga
    11221 accttcgctg ggttgagctt ggtaaagcaa gcgacgattg ctcagctccc ccgtgactcg
    11281 atcgtagtcg aactcgaaga tgcgggcggt cgtgctgcac gtccagaaga agcgcttcaa
    11341 atcgcgcgtg aaccccatgc cgttcgcgat ctgggtgccc ttccataggc agcgatagct
    11401 gccgtcagga tcgatccgat agaccccgcc actctcctgc gtgttgccga aggttccggc
    11461 aaatactcgc ccttccggat cggcgattac atcgttgaag cgcgccatgt cggggtcgat
    11521 tccctcgatc aacacgcgac gctgcccggt gcggaggtcg agccgagcga ttcgatcggc
    11581 ctcgaacagc agcaacgacc cgtcagcttg aagcgtgaag ccgccgatcg catcgccctg
    11641 ccagaagcac tcgtgcgcgc cgtcggcatg gcgcgcgcga aacagccgcc ccgtcgcgat
    11701 atcaacccag taaaggcagg cgtcgtccgg gttccagagc ggattttcac cgatctcgca
    11761 atgatagttc gctacggggc ggggctcgtt cgcgggcgca ttcatgggcg gtcgggttcc
    11821 tctcgatgat ccagcatagg taggtacgcc tcagaatagc ttgcggagcc gctccacggc
    11881 ttggctgacg ccctcgggga acgcgcgcaa gcgcacgtcg agatgatccc cttcggtttc
    11941 gatggcgccc accgtaacga gcggcacgat cgcggcgagc agccgcttga gcttgggatc
    12001 cgacttgagc ttggggtgcc tgaacaagtc gatgcggatt cgatcgtcct tggcttcggt
    12061 caaaatcgcc gggcgcttgg gcatataagc caccaggtta ccgggcttgc tcaaatcgag
    12121 cgcgccggaa cgtagcaagg ctgcaagcgg actctcgacc ttgtcgtccg tgacgcgcaa
    12181 gctgacgttc ttgaggcgca caccgaagct cagctcttca gcagtgatgg tgaccgaatc
    12241 gatgtagaca tccgccagcg cgcggagcgg ggtgcccatg agttcgaagc tcccgccgac
    12301 ccgaatccca ggtggaacgg cgctgatttc tacgtctttc ggcgccttct tgccgctggc
    12361 ttttcccgcc aaccagcgta acgccgccat tggcacgccg aagcgcaagc ccgcggcgtt
    12421 gcgcagtacg cgaatcactt cctcagggtg ctgccgcacg tacgcgaatg cagcttcgag
    12481 gacggatatg ccttccgaga gcagtttatc catggaacga cgatcgagcg aacccgcgcc
    12541 ggacgtattc ataggcgccg gagtaagcca caggtcgtcg ctccttgcga gagtactgat
    12601 gcctgggcgg ctgcaatttg tcccttgctt cgggttgtgt tctggtctcg cgctgtcctg
    12661 gcctcgtacc catacctgat tgggtctgga tctctggccc atcggccgag ttctcgagcg
    12721 actgggatga accgcgactc gataaaccgc tgcaggtcga ggcggtccat gattggaagt
    12781 ttgatcaagg cctacacagc gggcctagcg ccaaatgcta gtacaaggca tgagccacgt
    12841 agaacctgct gaagttcacg cgacgctcgg gcgccacctc atcgcctccg ggcatccgct
    12901 ggttttggat ctggcgcgca gtcagggcac cttcttgtac gacgctctag gtgggacgga
    12961 gtatctagat tttgccgggt tctatgcttc gaatgcgctc ggctttcgac atccctcgct
    13021 cgaaaccgaa acggctcggc agaagctgct cgagagcgcc gcgatcaagg tgggcaatcc
    13081 ggagttctat accgtggctt tcgcggagtt cgtagcggcg gtggagcgca ttgccgcccc
    13141 ctccacccat cctcattact tcttcgtgga ggggggtgcg ttggccgtcg agaacgcgat
    13201 gaaagctgcg atggactgga aggttcgtca caacttggcc cgcggcttgg gcgagattgg
    13261 cacggagatc ctgcatttcg agcgcgcctt tcacggtcgc gccggataca cgctgagcgt
    13321 caccaacacg gatcctgcca agactcaata tttcccgaag ttcgattggc cacgtgtccc
    13381 ctccccggcc ctgcgttttc cgcgctcggc cgagcacgac gcagccacgc tcgcggcgga
    13441 gcaggcctgt ctaagccaaa tcgagagcgc gttcgaggcc cggccgcatc gcatcgcggc
    13501 gattctgatt gaacccattc agggagaagg tggggacaac cacttccgcc cggaattcct
    13561 gcaggccttg cggcgcatcg cggatcaacg ggaagccctg ctgatcttcg acgaa
//
LOCUS       DASTKU010000865        15638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1286265, whole genome shotgun sequence.
ACCESSION   DASTKU010000865 DASTKU010000000
VERSION     DASTKU010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 15638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15638
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286265"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VFQ61_30465"
     CDS             complement(<1..929)
                     /locus_tag="VFQ61_30465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958869.1"
                     /translation="MQTELNRSYSHPGRIWPVLVLAGIAIACSSEGEGAAQSDSPRDE
                     ESSIDPEEYLRAEREAITSDTGIPVSISNPEETVNPAAAAVIPHGACLDGITNFSSAG
                     PFAVSTQRSGPVNFYVPGVPSGCKVPVVHLANGTGATCATYGATLRRLASHGFLAACY
                     ESTNTGAGDQGLEALDRALSAFPNLADNKFGSTGHSQGGQAAFTVLALAEKKYGERFI
                     YAGLAMQPASGFGAQPRGTTWRALYGSIRSPMFMFSGLGSDGLVSQSWVQSAYNATNA
                     QEEAYFWAKSGSRHIPVPNSETQQVAVPWFRWKL"
     gene            1467..2324
                     /locus_tag="VFQ61_30470"
     CDS             1467..2324
                     /locus_tag="VFQ61_30470"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9958870.1"
                     /translation="MADETDETDETLLERIRGGDPGALDQLIERHQQKLYRFGMKMCR
                     DPEDAKDVLQDSLLALARSIRDFRGDSSVSTWLYKVARSCCVKKRRSGRSVRTSDSLS
                     TSSVDSSLEREGSETPDESLAAREIRTVLDQAIATLEPAYREVLVLRDIEGLTAPEVA
                     EVVGLTVEAVKSRLHRARLMVRERVAPVLDLNSRSRAARAHCPDVLILFSRHLEGEIS
                     ADACAEVESHLRSCGACRALCDSLQHSLKLCRASQAHAPVPSAVQASVRVALRELLAA
                     QDGPLGTSP"
     gene            complement(2351..3565)
                     /locus_tag="VFQ61_30475"
     CDS             complement(2351..3565)
                     /locus_tag="VFQ61_30475"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HET9958871.1"
                     /translation="MEAPDIPEAQEPQATTNPSMLLNVGPAHPAMHGTVRIVIELSGE
                     DILRCDVQIGYLHRGFEKMFERGTWSQVFPYVDRANYCSPMLNNVGFALACEKLLGIE
                     TPERCQWYRMALGELSRITDHLTCDGAMAMELGAFTPFLWMMKTRDMIYDLMEEETGA
                     RLTVSFVRVGGMAAPPTPGFKDNARAVLNHLRSTVTEVKRLLLGNRIFLDRLEGIGKI
                     TREEAISLGWTGPCLRASGVPYDVRRAHPYLRYAEVDFDVPIGSEGDCYDRFMVRIEE
                     IEQSARIVEQCLRRMADSGPVDVNDPRIMLPPKEEVYDTIEGTIQHFKIIMEGLKLPA
                     GEVYGYTEGGNGELGFYLVSDGSGTPYRVRMRPPSFYITGGLERVITGQMLADVVPCF
                     GSINMVGGECDH"
     gene            complement(3761..4717)
                     /locus_tag="VFQ61_30480"
     CDS             complement(3761..4717)
                     /locus_tag="VFQ61_30480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9958872.1"
                     /translation="MPELADMSLFVRAALSGSLSAAGRALGFSPAVASKRLARLEAEL
                     GTRLIQRTSRRLKLTEEGATYLEFCRSILAQVDAAEVAVGRGRHQPSGTLRVSSPVAL
                     GRRWVGPTLGRFAAEYPHLSVQLALSDAMIDLLEQGFDCAVRIGHVEDSRLIARRLAD
                     NRRVVCGSPEYLARRGSPRIPADLYEHDCIVMSSGSVTHADWTFFDAHGPAKPTGSSA
                     TTVRVHGRWVSNSGEQAHDWALAGLGLERRSIWDVAQELADGRLIEVLSDWTSEPAPI
                     HVVFPSKSLLPARTRLFVDRLVTEFAQAQRAISAAGARATKD"
     gene            4803..5396
                     /locus_tag="VFQ61_30485"
     CDS             4803..5396
                     /locus_tag="VFQ61_30485"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET9958873.1"
                     /translation="MQKTNFRWHGFAALTALLWTGGVLAAKAKTPDPRTLSIDAALPV
                     PNTTLVDRSLPAEQVARMTLAARRYYGFWHTGRAELARAALASDFKDLNLPAGRPQGP
                     EGPLVASHSFRTAVPDLRLEVEEMLVIGDRVVGRLHFRGHFTGAFGTGEKARRGDGRS
                     VDFVAIDVYRIRGDRIAENWHLEDNLTLLHQLGLIQE"
     gene            5717..7918
                     /locus_tag="VFQ61_30490"
     CDS             5717..7918
                     /locus_tag="VFQ61_30490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HET9958874.1"
                     /translation="MRNLLRPTAQLSSGLLFGLLVAACGGEESGAPRRVTGTGGAPNT
                     VPANGGATSTLPTGTGGFIIGTSPPPDKPKPAGCGDGSLTSDEACDDGNTKSGDGCSD
                     TCLMVERGFSCASPGKPCREIARCGDGLVAASEQCDDGNVNPGDGCSERCRVELGKKC
                     EGSPSVCTEAKCGNGVVEGAESCDDGNNVPFDGCSALCLREPNCTGESCTSECGDGLL
                     INEECDDGNTIDGDGCSSTCTKETGFTCKQEAQCEKINDQCVLRVPTIFRDFSDKDAD
                     FGWPTYPDTTCGLKEGDPIVPGIAQDRLDAEGRPVLGKAPKEACIESSQSFARWFRDN
                     NGSVRVVGDIVLFDNGSGGYVNRFGQNGEKLITSVAPPKGEGQEQQVPNATSQATCEP
                     GCTARVRSSLQCDNLCRPQRDQVRTSGDVLRQRQTELVQRQDQLEQRQAADPPDEAAI
                     ALLEEQIADLETEIAELQADIEALTATADTCETDCQTRFDGQVAACVADCKPCSTNAA
                     QWCTGGKIVEFDGTPLFFPVDSVTGPTRDSDFAQLPLQYGYSGWPSEDKVFPGAKQHN
                     FYFTSEVQYWFRYEQGTNARLDFTGDDDVWVYVNGRLAVDLGGIHVPMDGSVTINAQS
                     ATKFDLKPGNVYKIVVFQAERKKFGSSFRLTLSGFEATPSDCSAVCGDGILSFGEECD
                     DGVNDGGYGECDVGCKLGPFCGDGIVQEPEHCDNGPGDSDCPGCRKLEPPR"
     gene            complement(8045..9277)
                     /locus_tag="VFQ61_30495"
     CDS             complement(8045..9277)
                     /locus_tag="VFQ61_30495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958875.1"
                     /translation="MAAQNDSDFAGYSVLYAERFTGVKRSGAYRATFDRENWLKDRSF
                     MFRVPVRVSMNNLQLSLTSAGGVARFEQTWASARYKDVGIKQMVIVPTRDGPRIAREE
                     MLTSRIDEQHVASRTSSRDMRIMTRDGVLLDETGEGSWAVGAVQSGRDNYAVLRDLDS
                     TKLPAELRALEGQAVQVLGTSEVVCQAKIAGFKLRSHVVPHFGMEHDWAETKANVPEE
                     LWKLGESSRQVIGILEPSCEHGLWALPAQKRAPALAAPKAVEGSLRLAALKAFRALPA
                     YRENQQEYVRFHPGVKGTWDEYEGARAEVFGFGTGDSISVVSVSVRAGQGCGEFSGEL
                     SARFAAVPRANGVQLKLLESSPEDVNPLSAIDFEGDGLPEILFESRGGTTRSLWQTKG
                     TSAELVPLLGVLSLDCPC"
     gene            complement(9722..10963)
                     /locus_tag="VFQ61_30500"
     CDS             complement(9722..10963)
                     /locus_tag="VFQ61_30500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HET9958876.1"
                     /translation="MRILSPQSYILGAILSVVGCSANGGDPILGQGGSVAPGGSGAGG
                     AKTTGGTTGSASGGQTTGAGGFVSPTGGTVATGGIGSGGSSQTKGGNTGFPNTGGSGT
                     GGFQANGGSGNSGGASGASGGKSNAGGFGNDGGKSNSGGFGNTGGVGNGGSGSGGRTF
                     GNGGSGNGGSGNGGSGNGGSGNGGSGNGGKANSGGTTGAAGSGAGGMSSGGGVPTGSC
                     SEGQKSFTNNATGTHCGYTYEYWKDNGTGTLTVKPDGFSVNWSNIGNLLGRKGLRPGG
                     KSNVVTYESNYQPNGNSYLCVYGWTRSPLIEYYIVDSWGSWRPPGGQGLQGTVTCDGG
                     TYDIYKTQRVNQPSIDGNTTFYQYWSVRQQKKTSGTITVGCHFDAWAAKGMNLGSFYE
                     VSMTVEGYQSSGTADVKFSMK"
     gene            complement(11664..15083)
                     /locus_tag="VFQ61_30505"
     CDS             complement(11664..15083)
                     /locus_tag="VFQ61_30505"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET9958877.1"
                     /translation="MKSSVIDSASVPVQTFSPQPSGQGAFRAVNKIRLVTATSLFDGH
                     DAAIHIFRRIAQASGAEVIHLGHNRSVREIVDAAIQEDVQGIAVTSYQGGHNEFFRYM
                     VDCLRARGCEHVRIFGGGGGVILPSEIQALQEYGVCRIYSPDDGRALGLQGMVEDLLS
                     HCDFAVVQDEQAFPRPHTDDPRAIARAISLVESQPERHLEVLRSMREQLAPAPLVLGV
                     TGTGGAGKSSLVDELLRRFLADFSEHRVAVLCVDPTRRKTGGALLGDRIRLNSAADPR
                     VFVRSMATRRSNLALGNHVREALDVLKVAGFDLIVLESSGIGQSDTEIVEHSDVSLYV
                     MTPEFGAQTQLEKIDMLDFADWVAINKFDRRGARDALRDVSKQVQRNTRAFEVGWEQM
                     PVFGTMAASFADPGTNRLYRAIVGRLQTSRPGHFQPTIELELGEPRTASLLPAERQNY
                     LAEIVEDSRRYQAWTVEQSEIAERCYVLESAAAALPESAGAARAALMSELTEARRRQA
                     PECRVLLDGWESMTAELEREHSTFRVRDREISVENHSVSLSGLAIPKVAPPQFRSPAD
                     RLRWLLTENLPGRFPFTAGVYPFKRTDEVATRMFAGEGGPERTNRRFHYVSSGQPFKR
                     LSTAFDSVTLYGADPAERPDIFGKIGNAGVSVCTLDDAKRLYSGFDLCDSKTSVSMTI
                     NGPAPMLLAYYLNAAIDQQCERWLLQNARSEAADRRIEALYRDRGLPRPRYRAELPTG
                     HDGGGTLLLGMTGDLLVPPEVYERIRAEALSVVRGTIQADILKEDQAQNTCVFSTEFA
                     LRMMGDIQQFFTQNRVRNFYSVSVSGYHIAEAGANPITQLAFTLANGFTYVEYYLARG
                     LRIDEFAPNISFFLSNGLDPEYAVLGRVARRIWAKAIKHRYAGNERSQLLKYHIQTSG
                     RSLHAQEIEFNDIRTTLQALYAIYDNCNSLHTNAYDEAITTPTESSVRRALAIQWIIN
                     QELGLARTENPLQGSFVVRELTDLVEAAVYAEFLRLSERGGVLGAMETMYQRSKIQDE
                     SLHYEVLKSTGKLPLVGVNTFLGPDGSPIQSLGEVVRSTESEKRAQIESLSQFHAFHG
                     ERATQALRELELAALRGENVFEPLMEACKVASLGQISESLYRVGGQYRRNV"
BASE COUNT         2964 a         5005 c         4702 g         2967 t
ORIGIN      
        1 agcttccagc gaaaccacgg caccgcaact tgctgggtct cggagttcgg aaccggaata
       61 tgccgagagc ctgacttggc ccaaaagtac gcctcctctt gggcgttcgt cgcgttgtaa
      121 gcggattgta cccacgattg ggaaacgaga ccgtccgatc ccagcccgct gaacatgaac
      181 atcggagatc ttatgctgcc atacaaagct cgccacgtgg tcccccgggg ttgggcgccg
      241 aagccgctgg cgggctgcat cgcgagaccg gcgtagatga aacgctctcc gtatttcttc
      301 tcggcaaggg cgagtacggt gaacgccgcc tgcccacctt gtgaatgtcc cgtggagcca
      361 aacttgttgt ctgccaagtt ggggaacgcg gagagcgcgc gatccaaggc ttccaggcct
      421 tggtcacccg caccggtatt tgtgctttcg tagcacgcgg cgagaaaccc atgagacgct
      481 agccgacgca gggtcgcccc gtatgtcgcg caagtcgcgc ccgtgccgtt tgcgagatgc
      541 acgactggca ctttgcaacc gctgggaaca cccggcacat agaagttgac cggaccagat
      601 ctctgagtgc tgaccgcgaa ggggccagcg ctcgaaaagt tggtgatgcc atccagacat
      661 gcaccatgcg ggatgaccgc ggcagcggcg ggattgacag tctcttctgg gttcgagatt
      721 gagactggta taccagtgtc gctggtaatt gcctcgcgtt cggcacggag atattcctca
      781 gggtcgatac ttgattcctc atcccgtgga gagtccgact gcgccgcccc ctctccttcc
      841 gaggaacacg cgatggcgat gcccgcgagc acgaggactg gccagatgcg acctgggtga
      901 gaatagcttc tattcagctc tgtttgcatc gctattacag tgtcaatcgc tggtccgagc
      961 gggtagcgtg cctcgagatt gccccgcaaa gccgatcacg cccgtcgtga accatggatg
     1021 gggttcgata tcgggtttgc cttgccgtaa tgcttccatt tgcccttgct tgatccaact
     1081 cgggacgtga tgctttggct ggacgcggaa cgggccttac aggttgggac gctgggtgcg
     1141 gatcagcagc ccggtctgga acgcgctctc atcttccctg tcaggacgtc atcccgctcg
     1201 gcgagcagat tctggccact cccgcgggga tgctcgttgc ttggcgatcc ccactcggcg
     1261 ggcgtatctg acaccgctct gccacaagta ctacttgcga tgcagaggtt cgcggtgctc
     1321 acgctcgaca tgacgaatcg caactatcgg cccctaaact gacgccgcct attacacagt
     1381 agagccgctt ggcgcatgta gaccgagcgg cgacggcatt tgccgttggc agaaggccga
     1441 caactatggg aattcaggtc tggcgcatgg ccgacgagac cgacgagacc gacgagaccc
     1501 tcttggaacg aattcgtggc ggcgatccag gcgccctgga tcagctgatc gaacgtcatc
     1561 agcagaagct ctatcgattc ggcatgaaga tgtgccgcga tccggaggat gccaaagatg
     1621 tcctccagga tagtctgcta gcacttgcgc gctcgattcg ggacttccgc ggtgactcgt
     1681 cggtctcgac ctggctttac aaggttgcgc gcagctgctg cgtcaagaaa cgccgttcgg
     1741 gccggtccgt gcgcacgagc gattctctca gcacgagctc ggtggactcg agcctggagc
     1801 gggagggctc ggagaccccc gatgagtcac tagccgcacg cgagattcgg actgtgctcg
     1861 atcaggccat cgcgacgctc gaaccagcgt atcgcgaggt cctcgtactg agagacatcg
     1921 aggggctcac cgcgccggag gtcgcggagg tcgtgggtct caccgtcgaa gctgtgaaga
     1981 gccgcctcca ccgtgcgcgc ctgatggttc gtgagcgcgt cgcacccgtg ctcgatctga
     2041 actcgagatc tcgcgccgcc cgcgcgcact gccccgatgt gctgattctg ttctcacgcc
     2101 acctcgaggg cgaaataagc gcggacgcct gcgccgaagt ggagagtcac ttacgctcgt
     2161 gcggcgcctg tcgagctctc tgcgactcgc tacaacacag cctgaagttg tgtcgagcca
     2221 gtcaggcgca tgcgccggtc ccttcggccg tgcaagcctc cgtgcgcgtc gcgctccgcg
     2281 agctcctcgc ggcccaagac ggcccgctcg gcacctctcc ttgatacgat gtcgccaggt
     2341 tggtcacggg tcagtggtcg cactcgccgc caaccatgtt gatcgagccg aagcacggca
     2401 cgacgtccgc gagcatctgc ccagtaatga cacgctcgag gccgccggtg atatagaaag
     2461 atggtgggcg catgcgaaca cgatagggag ttccactacc gtcactgacc aagtagaagc
     2521 caagctcgcc gttgccgccc tctgtgtacc cgtagacctc gccggccggg agcttgagcc
     2581 cctccatgat gatcttgaag tgctgaatgg tgccctcgat tgtgtcgtac acctcttctt
     2641 tggggggcaa catgatgcgc ggatcgttta cgtctacggg tcccgaatcg gccattcggc
     2701 gcagacactg ctcgacgatg cgtgcgctct gctcgatctc ttcgatgcgg accatgaaac
     2761 gatcgtagca atccccctcg gaaccaatgg gcacatcgaa atccacctcc gcatagcgga
     2821 gatagggatg tgcccgacgc acgtcgtaag gcactccgga cgcgcgcaga cacggaccgg
     2881 tccaacctag ggagatggcc tcttcccgcg taattttgcc gatgccctcg aggcgatcga
     2941 ggaagatgcg gttcccgagc aacagcctct ttacctccgt gacggtcgac cgcaggtgat
     3001 tcaacacagc ccgggcgttg tccttgaatc ccggggttgg gggggccgcc atgccgccca
     3061 cccgtacgaa gctcaccgtc aagcgggcac cggtttcctc ttccatcaag tcgtagatca
     3121 tgtcccgcgt cttcatcatc cacagaaaag gcgtgaaagc tcccagctcc atcgccatcg
     3181 ccccgtcaca ggtgaggtga tcggtgatgc gcgacagttc ccccaacgcc atccggtacc
     3241 attgacaccg ctccggagtt tcgatcccga gcaacttctc gcacgccaac gcgaatccga
     3301 cgttgttgag catcggtgag cagtagttgg cgcggtccac gtacgggaat acttgggacc
     3361 aggtgccccg ttcgaacatc ttctcgaagc cgcggtgcaa gtaaccaatc tgaacatcgc
     3421 agcgcaaaat atcctcgccg ctcagctcga tcacgatccg aaccgtgccg tgcatggcgg
     3481 ggtgagctgg gcctacgttg agcagcatcg acggattggt cgtggcctga ggttcttgtg
     3541 cttcggggat atcgggcgct tccatgcttt gggctctctc gcgcatcagg ctgccgcatc
     3601 ctcctcgctg cgtatgcacg cagcatcgcc gacgcctgtc cgcgtttgtc ccacgcgagt
     3661 acgcactccc gttcatggca gtgcgggaaa tgccacaaac gcccacgccc gttccgcttc
     3721 cgtccgtcgc cagcgactca ccgcgaggca cgttggcgcg ctagtccttc gtggccctcg
     3781 ctcccgctgc ggagatcgcc cgctgagcct gcgcaaactc cgtcacgagg cgatcgacga
     3841 agagccgagt gcgagcaggc aagagcgact tggacggaaa gacgacatgg atgggagcgg
     3901 gctcgctcgt ccagtcgctc agaacctcga tcaagcgtcc gtccgcaagc tcctgcgcga
     3961 cgtcccagat cgatcgccgt tcgagcccga gccccgcgag agcccagtcg tgtgcctgct
     4021 caccgctgtt gctcacccaa cggccatgca cccgaaccgt ggttgccgac gacccggttg
     4081 gcttcgctgg cccatgtgcg tcgaagaagg tccaatcggc atgcgtcacc gaacccgagc
     4141 tcatcacgat acaatcgtgt tcgtacaaat cagccgggat tcgcggcgag ccgcgcctcg
     4201 cgaggtactc cggcgaaccg caaaccacgc gccgattgtc cgccagccgg cgggcgatca
     4261 atcgcgagtc ttcgacgtgc ccaatgcgaa ctgcgcaatc gaagccctgc tcgagcaaat
     4321 cgatcattgc atccgatagt gccaactgga cgctcaagtg cggatactcc gcggcaaaac
     4381 ggccaagcgt cgggccgacc cagcggcggc cgagagcgac cggtgaggat acgcgcagag
     4441 ttccgcttgg ttgatggcga ccgcgaccga ccgcgacttc cgccgcgtcc acttgggcca
     4501 atatgctgcg acagaattcc aagtacgtcg caccttcctc ggtcagcttg agccgccgcg
     4561 acgtacgctg aatcagccga gtcccgagct cggcttcgag gcgcgccaaa cgtttgctcg
     4621 cgactgccgg ggaaaagccg agcgccctgc cggccgcaga gagactgcca ctcagcgcgg
     4681 cgcgcacgaa cagggacata tcggcgagtt cgggcattcg gggatcttca acgaatcgga
     4741 acagctggtt ctgcgacgac gtgcattctc aaacccggct ccgacggcag acaatcctgc
     4801 ccatgcaaaa gacgaacttc agatggcacg gtttcgcggc gttgacagcg ctactctgga
     4861 cgggaggagt gctggctgcc aaggcgaaga ctccagaccc acgaacactg tccatcgatg
     4921 cggcgctgcc ggtgcccaac acgaccctgg tggatcgctc cctcccggcc gagcaggtcg
     4981 cccgtatgac cctcgcggcg cgtcgctact acgggttttg gcacaccggg cgtgcggagc
     5041 tggcgcgggc ggcgctcgcc tcggatttca aggacctcaa cttgccagcg ggtcggccgc
     5101 aaggacccga aggaccactg gtcgcatcgc acagcttccg tacggccgtg cctgaccttc
     5161 ggctcgaggt ggaggaaatg ctcgtcattg gcgaccgcgt ggtgggacgt ctgcactttc
     5221 gcggccactt taccggagcg ttcggcactg gcgaaaaggc ccgacgcgga gacggccgca
     5281 gcgtcgactt cgtggcaatc gacgtatata gaattcgggg ggatcgcatc gccgagaact
     5341 ggcacttgga agacaatctg actcttttgc atcagctagg gctgatccaa gaatgaggct
     5401 catcgcgcga tgaaaccccg ccgagcggca gggcagtcca gaaagagagc acgcgcacga
     5461 ctgccccgcc ctcgagtact ccgctgctgc cgtccagttc cctcccattc cgatcggcgc
     5521 gctgacgatt caatgcgctg aaacgcccga aaactagatg ccccgaacca tgttctggac
     5581 gggcgcgccc ccaagcacag ccagcgcgaa gaattggaga gtgaatagac ggatatgcat
     5641 cattgaaccc cgcaaagctc atggcttccc gccgctcccc gtgtcagtgg agaggctgca
     5701 cctacgggtg gtcgccatgc gcaacttact tcgtccaact gctcaacttt catcagggtt
     5761 gctcttcggc ttgctggtcg ccgcgtgcgg cggggaggaa tccggggctc ctcggcgcgt
     5821 cacgggtaca ggaggtgctc ccaacaccgt ccccgccaac ggcggagcga cgagcactct
     5881 tcccaccggc acgggcggct tcatcatcgg cacttccccg ccgccagaca aacccaagcc
     5941 tgccggctgc ggggacggca gtctgacgtc cgacgaagca tgcgacgacg gaaacaccaa
     6001 gtcgggtgac ggctgcagcg acacctgtct catggtcgag cgcgggttca gctgcgccag
     6061 ccctggcaag ccctgccggg agatcgcgcg ctgcggagac ggtctcgtcg cggccagtga
     6121 gcagtgcgac gacggaaacg tcaaccccgg tgacggctgc tcggaacgtt gccgagtcga
     6181 actcggaaag aagtgcgaag gttcgccgag tgtctgcacg gaagccaagt gtggcaacgg
     6241 cgtcgtcgag ggcgcagaga gctgcgatga tggcaacaac gttccgttcg acggctgctc
     6301 ggcgctttgc ctgcgtgagc cgaattgcac cggagagtcg tgtacgtccg aatgcggcga
     6361 cgggctgctc atcaatgagg agtgcgacga cggcaatacc atcgacgggg acggttgctc
     6421 ctcgacctgc accaaggaaa cggggtttac ctgcaaacag gaagcgcagt gcgagaagat
     6481 caacgatcaa tgcgtgctgc gggtgcccac gattttccga gacttttctg acaaagatgc
     6541 ggactttggt tggcctacgt atccagacac gacctgcgga ttgaaagagg gtgacccgat
     6601 cgttccgggc atcgcgcagg accgtctgga tgcggaaggg cgcccggtgc tcggcaaggc
     6661 ccccaaggaa gcctgcatcg agtcaagcca gagcttcgcg cgttggttcc gcgacaacaa
     6721 cggatcggtg cgggtagttg gcgatatcgt gttgttcgac aatggcagcg gcggttacgt
     6781 gaaccgcttt ggccagaacg gcgagaagct gattaccagt gtggcgccgc ccaaaggcga
     6841 gggccaagaa cagcaagtcc cgaatgccac gagccaagcc acgtgcgagc ctggctgcac
     6901 cgcgcgagtg cgaagttcgc ttcaatgcga caacctgtgc cgcccccagc gcgatcaggt
     6961 ccgtacctcc ggggatgtgc tccggcaacg ccaaaccgag ctcgtccaac ggcaggatca
     7021 actcgagcaa cgccaggccg cggacccgcc ggatgaggcc gcgattgcgc tactcgagga
     7081 gcagatagcc gatctcgaga ccgagatcgc ggagttgcag gcggacatcg aggcactcac
     7141 ggccaccgcc gatacttgtg agaccgattg ccaaacccgg ttcgacggtc aggtcgccgc
     7201 gtgcgttgca gactgcaagc cctgcagcac gaacgccgcc caatggtgta ccggcggaaa
     7261 gatcgtggag ttcgacggca cccccctctt cttcccggtg gacagcgtca cgggccccac
     7321 gcgcgattcg gacttcgcgc aacttccgct gcaatatgga tacagcggtt ggccttcgga
     7381 agataaagtg tttcccggcg ccaagcagca taacttctac ttcaccagcg aggtgcagta
     7441 ttggttccgc tacgagcagg gcaccaacgc gcgtctggat ttcacgggcg acgacgatgt
     7501 ttgggtgtac gtgaatggcc gtctggccgt cgatctcggc ggcattcacg tgcccatgga
     7561 tggctcggtc actatcaatg cgcagtctgc aaccaagttc gacctcaagc ccgggaacgt
     7621 ctacaagatc gtcgtgttcc aagccgagcg caagaagttc ggctcctcgt tccgcctcac
     7681 gctttccggc ttcgaggcga cgcctagtga ctgcagcgcc gtttgcggcg atggcatcct
     7741 gagcttcgga gaggagtgcg acgacggcgt gaacgacggc gggtacggcg agtgcgatgt
     7801 cggctgcaag ctcggcccgt tctgcggcga tggcatcgtt caggagccgg agcactgcga
     7861 caacgggcct ggcgactcag actgccccgg ctgtcgtaag ctggaacccc cgcgctgaac
     7921 tcgaggctca tagtttcctc ggtttcgagg cttcgccttc actggccatt ttcgtggagg
     7981 cgaagtccgc accgagtgag ctcggccgtg atccgtctgg ctgacgcttg gcgcagcctt
     8041 ctggtcagca aggacaatcg agagaaagaa caccgagcag cggcaccagc tccgcgctcg
     8101 tgcccttcgt ttgccagagc gatcgcgtgg ttccaccccg cgattcgaat aggatctccg
     8161 gcaggccatc cccctcgaag tcgatcgctg acagcggatt cacgtcttcc ggtgagctct
     8221 cgagcagctt caattgcacg ccgtttgcgc gcggtacggc tgcgaatcgc gcgctcagct
     8281 cgcccgagaa ttcaccgcag ccttgacccg cccgtacgct gacgctcaca accgagatag
     8341 agtcgccggt accaaaccca aagacctccg ctcgggcacc ctcgtattcg tcccaggtcc
     8401 ccttcactcc cgggtggaat cgcacatact cctgctgatt ttctcggtac gccggcaggg
     8461 cccgaaatgc cttcagagcc gccagccgca ggctaccttc cacggccttt ggcgccgcaa
     8521 gggcgggtgc gcgcttctga gctggcaacg cccagaggcc gtgttcgcac gagggttcca
     8581 ggatgcctat cacttggcgg ctcgactctc ccaatttcca gagctcctct ggcacgttgg
     8641 ccttggtctc ggcccagtcg tgctccatcc caaagtgcgg aaccacgtgc gatcgcagct
     8701 tgaacccagc aatcttcgcc tggcagacga cctcggacgt accaagcact tgaaccgcct
     8761 ggccctccag ggcacgcagc tccgcaggca gcttggtcga gtctaggtcg cgcaacacgg
     8821 cgtagttgtc gcgcccgctc tgcacggccc caaccgccca gctgccctcc cccgtctcgt
     8881 ccaaaagaac gccgtcgcgg gtcatgattc gcatatctcg actgctcgtt cgcgaggcta
     8941 catgctgttc gtcaatccgc gaagtcagca tttcctctcg cgcgatgcgt ggcccgtcac
     9001 gagtgggcac aatcaccatc tgtttgatgc ccacgtcttt gtaccgggca gatgcccaag
     9061 tttgttcgaa tcgcgcaacc cctccggccg aggtcaggct aagctgcagg ttattcatgc
     9121 tcacgcgcac gggcacccgg aacatgaatg agcgatcctt caaccagttc tctcgatcga
     9181 aggtcgcgcg ataagccccc gagcgtttca ctcccgtaaa gcgctctgcg tagagcaccg
     9241 agtaaccggc aaagtcactg tcgttttgcg ccgccaacca taactcgagt actcgccgcg
     9301 tccccgcctg atccaggacc ggtagtgggg ttatcgagtg cggtacattc gagttcggac
     9361 gctcggcagc gcccctggcg gaagcagcgg ccgcagaatc gaggggcgat ctgggcagtg
     9421 ccttatgctc acctgtaggc gcttgcctct tcgagcacgc gacacccgtg aacgcgagag
     9481 ccatcgttaa aatcacaatc ccatgccggc acccaagatg cttggaaaca atggccgcta
     9541 gtcccacgat tctcgttccc tgtcactgct gatgcgtcga ctatcacctg cgacgcctga
     9601 aaacacgacc gccctcccca gacttgccgg agagggcggt ttcttgatcg aagtgctcga
     9661 gtcgaggtgc ccgaaatcgg aaggtcctga ctcgcgtgca cgagtgcgga cctttcgatt
     9721 ctcacttcat cgagaacttc acgtcggcgg tgccgctgct ttggtaaccc tcgacggtca
     9781 tggagacctc gtagaagcta cccaggttca tgccctttgc cgcccacgcg tcgaagtggc
     9841 aacccaccgt gatcgttccg ctcgtcttct tctgctgacg aacgctccag tactgataga
     9901 acgtggtgtt cccgtcgatc gacggctggt tcacgcgctg agtcttgtag atatcgtatg
     9961 tcccgccatc gcacgtcacc gtgccctgga ggccctgacc acccggcgga cgccagctgc
    10021 cccagctatc cacgatgtag tactcaatca acggactacg ggtccagccg tagacgcata
    10081 gatacgagtt gccgttgggt tggtagttgg attcgtacgt gaccacgttg ctcttgccac
    10141 cggggcgcag acctttgcgg ccgagcaggt taccgatgtt gctccagttc acgctgaagc
    10201 cgtctggctt gacggtcaga gtgccagttc cattgtcttt ccagtactcg taggtgtaac
    10261 cgcagtgagt gccggtcgcg ttgttggtga acgacttctg cccttcgctg cacgaaccgg
    10321 taggaacgcc gccgcccgag ctcataccgc cggcgcccga accggcagct cccgtcgtac
    10381 cgcccgagtt ggccttgccg ccgttgccgc tgccgccgtt gccgctgccg ccgttgccgc
    10441 tgccgccgtt gccgctgccc ccgttgccgc tgccgccgtt gccgaacgtc cttccgccgc
    10501 tcccagagcc gccatttcca acgccaccgg tgttgccgaa accaccagaa ttggactttc
    10561 cgccgtcgtt gccgaagccg ccggcattgg actttccgcc actcgcgccc gaggcgccgc
    10621 cggaattgcc agaaccgcca ttggcttgga agccacccgt tccggagccg cccgtgttgg
    10681 ggaagccggt gtttccaccc ttcgtctggg agcttccgcc cgagccgatt cctccggttg
    10741 caacggtccc accggttggg ctgacgaagc cgcccgcccc ggtggtttgc ccaccgctcg
    10801 cggagcccgt cgtgccaccg gtggttttcg cgccgcccgc tccggaaccg ccaggggcca
    10861 cggagccgcc ttggcccaga atggggtcac cgccgttcgc actgcaaccg acgacggaaa
    10921 gaatagcgcc tagaatataa ctttgcggtg atagtatgcg cacgagaata cctcgtttga
    10981 gttgcgatga ctggcgaagc tcaaatgcct gggcccttcc gaggcgaagc ttaatgccct
    11041 ttgagcggtc caggagatca cagcagatca cagcagatca gtcgcgatgt cctgacaagt
    11101 gcatcgtagc ctgtctgcca cttgagaagg ccatgaaagt gctcgccgca caccaaatat
    11161 tgaaaaaagt ggccagttcc ccgagatttt ttccactctc ctccacttct tccagttgtt
    11221 cggtactagc gtggacctga gtgatacggg catcgcaatt gacgagcttc ggagtgtatg
    11281 gcggcgatcc acagaacagc actggcttgc tcacgcgtga gtggttgtgc acggctcgag
    11341 tcagacggat tcggcatcac acagccacac agcacttcac gcgtcacacg gcacttcacg
    11401 taatcacgca gcacctcacg taattgccaa tccgcgggga ctcgcctacg ggcatcgttg
    11461 cgttgggggc tcgcggcaga agcctcgcat cagcataggg ccacgcgctc cccctatcac
    11521 gcggcaacgg cgggcaagcc ccagagtgtc tggatttgcg ccacttcctc gcttgcctct
    11581 tcgtcgcttg cggcagtgcg gagcgaagct cgtggcaaag cgtaacccgc gcaaccacgg
    11641 cgactcccgc ctgcgggtgc cgctcagacg ttccggcgat actgtccccc cacccggtag
    11701 agggattcgg aaatttgccc cagggaagcc actttgcagg cctccatcag cggctcgaac
    11761 acgttctcgc cgcgcaacgc cgctagctcg agctcgcgta aagcttgagt ggcgcgctcc
    11821 ccgtggaagg cgtggaactg actcagcgac tcgatctggg cgcgtttttc agactcagta
    11881 ctacgcacga cctcgcctag gctttgaatg ggggagccgt ccggccccaa gaaggtgttc
    11941 acccccacga gtggcaactt gccggttgac ttcaggactt cgtagtggag agactcatct
    12001 tgaatcttgc tgcgctgata catcgtttcc atggctccga gcacgccgcc ccgctcggat
    12061 agccggagaa actcggcgta caccgccgct tcgaccagat ccgtcagctc tcggaccacg
    12121 aagctcccct gtagtgggtt ctcggttctt gccagaccga gctcctggtt gatgatccac
    12181 tgaatggcca aagcgcgacg caccgagctc tcggtcggtg tggttatggc ttcgtcgtaa
    12241 gcattggtat ggagcgaatt gcagttgtcg tagatcgcat acagcgcctg cagcgtggtt
    12301 cggatgtcgt tgaactcgat ttcttgcgca tgcagcgagc gacccgaggt ttggatgtgg
    12361 tacttgagca actgactgcg ttcgttgccc gcataccggt gcttgatggc cttggcccag
    12421 attctccgcg cgacccggcc gagcacagca tattccgggt ccaagccgtt gctgagaaag
    12481 aagctgatat tgggcgcgaa ttcgtcgatc cgtagcccgc gcgcgagata atactccacg
    12541 taagtgaagc cattcgcgag cgtgaaggcc aattgggtaa tgggattcgc cccagcttcg
    12601 gcgatatggt atcccgacac ggacaccgaa tagaagttcc gcacgcgatt ttgcgtgaag
    12661 aattgctgaa tatctcccat catgcgcaac gcgaactcgg tgctgaatac acaggtgttc
    12721 tgtgcctgat cctccttgag gatatccgcc tgaatagtac cgcgcaccac gctcaaggcc
    12781 tcggcgcgaa tacgctcgta cacctcgggc ggcaccaaga gatcgcccgt catgccgagc
    12841 agaagcgtgc ctccgccatc gtgacctgtc ggcagttctg cgcggtaacg cgggcgcggc
    12901 agccctcgat cgcgataaag tgcctcgatc cgacggtcgg ctgcctctga ccgagcattc
    12961 tgcaatagcc agcgctcaca ctgctgatcg attgccgcgt tcaagtagta cgccaacagc
    13021 atgggcgcgg gcccattgat ggtcatggac accgaggttt tcgaatcaca caagtcgaag
    13081 cccgagtaca agcgcttggc atcgtccaga gtgcacaccg agactccggc gttgccgatc
    13141 ttgccgaaga tgtcagggcg ttccgcggga tccgctccgt acagggtgac gctatcgaac
    13201 gccgtcgaga gccgcttgaa gggctgcccg gaagaaacgt aatggaatcg acgattggtg
    13261 cgctccgggc cgccttcgcc ggcgaacatg cgagtggcta cttcgtcggt tcgcttgaac
    13321 ggatagacac cggccgtaaa cggaaagcgc cccggcaggt tctccgtcaa caaccagcgc
    13381 agtcgatcgg caggcgatcg aaactggggc ggtgcgacct tgggaattgc caggcccgag
    13441 agcgacaccg agtgattttc gacgctgatc tcgcgatcgc gcacccggaa ggtcgagtgc
    13501 tctcgttcga gttccgcggt catgctctcc catccatcca agagcacgcg acattcgggg
    13561 gcttgacgcc gccgggcttc cgtcagctcg gacatgagtg cggccctcgc agctcccgct
    13621 gattcgggca gcgcggccgc tgcactctcc aacacgtagc aacgctccgc gatttcactc
    13681 tgctcgacgg tccaagcctg atagcgacgg ctgtcctcca cgatctcagc caagtagttt
    13741 tggcgctcgg ccggtaacag cgatgccgtt ctgggctcac ccagctcgag ctcgatcgta
    13801 ggttgaaagt gccccggacg ggaggtttgt agtcggccga cgatggcgcg atacagtcgg
    13861 ttggtacccg gatccgcgaa gcttgcggcc attgttccga agaccggcat ttgctcccat
    13921 ccgacctcga aggcgcgagt gtttcgctgc acctgcttgc tcacgtcacg cagggcatcg
    13981 cgcgcgcccc ggcgatcgaa cttgttgatc gccacccaat cggcaaaatc caacatgtcg
    14041 atcttctcga gctgggtttg ggcgccgaac tcaggagtca tgacgtagag cgagacgtcg
    14101 gagtgctcta caatctcggt atcgctctgg ccgatgccgc tgctctcgag cacgatcaga
    14161 tcgaaacctg ccactttcaa cacgtcgagg gcttcgcgga cgtggttgcc gagcgccagg
    14221 ttggaacggc gcgtcgccat cgagcgcacg aacacgcgtg gatctgcggc gctgttcaag
    14281 cggatgcggt cacccaacaa agcgccgccc gttttgcgac gcgtaggatc gacgcacagg
    14341 accgcgacgc gatgctccga aaaatccgcg aggaagcggc gcaatagctc gtcgactagg
    14401 ctgctcttac ccgcgccgcc ggtgcccgtc acgccgagca cgaggggcgc cggtgctaac
    14461 tgttcccgca tcgagcgcag cacctcgagg tggcgctcgg gttgagattc gaccagcgaa
    14521 atggcccgag cgattgcgcg cgggtcgtcg gtatgcggtc gcgggaaggc ttgttcatcc
    14581 tgcacgaccg cgaagtcaca atgggacagc aggtcctcga ccatgccctg cagcccaaga
    14641 gctcgtccat cgtccggcga atagatccgg cacactccgt attcctggag ggcctggatc
    14701 tcggacggca ggatcacgcc gcctccaccc ccgaaaatcc gcacgtgctc gcagccacgc
    14761 gcgcgcaagc agtcgaccat gtatcgaaag aactcgttgt gcccgccttg gtacgaggtg
    14821 acggcaatcc cttggacgtc ttcctgtatt gccgcgtcca ctatctctcg aacggagcgg
    14881 ttgtgcccca ggtgaatcac ctccgctccc gaggcctgcg cgatgcgacg gaagatatgg
    14941 atggccgcat cgtggccgtc gaagaggctg gttgccgtga ccaacctgat cttgttcacg
    15001 gcccgaaagg caccctgccc ggagggctga ggagagaagg tttgaaccgg aacggaggct
    15061 gaatcaatca cggagctctt cacgggaagg ggaccctcgc tgtgcgcggc agtcttccag
    15121 atccgacgca ggtcggcgac tctttggcca gcgcgcagga cccggaggtg gtttcgtggg
    15181 tggtttgccc gaatggacgt ttgcagcagc ggccctccgc cagcctcact cacccggctc
    15241 agcttggccc ggctcagctt ggcccggctc agtttggcat gctcaagccc gggcctggct
    15301 catattggcg aggctccgtt tgccgcggca ccggctttga ctcggaccgc ccgagagcca
    15361 gactgccggc gctcgcagcc tctaccgcgt cgttggaatg gcgcgttgca acctgagcgc
    15421 gattggccaa accgagcaaa ccggatcggc agctgagtcg tgcaacctgc gggggcgcgc
    15481 aaacaccccg cgggtggaga aaaaattagt cctcagtgag cgcgcataaa gcgctttcgg
    15541 atttgagctc gacgaaagtt gatccagcct tcggtcgagg tcgccgactc ttgaaaaaac
    15601 tgtgctcttc cttggttctc atatgcctgg tgggttgc
//
LOCUS       DASTKU010000866        17223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1732176, whole genome shotgun sequence.
ACCESSION   DASTKU010000866 DASTKU010000000
VERSION     DASTKU010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 17223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17223
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732176"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1378)
                     /locus_tag="VFQ61_30510"
     CDS             complement(164..1378)
                     /locus_tag="VFQ61_30510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958878.1"
                     /translation="MKRFGHDMVSHLSLTLCLAAFPAILMLGCGGSDEPSAGKGGSSS
                     SQGGSGAGSAQTGGTSAGAPAASGGASTSGGASTVGASGGAASGGRSNGPMGGSTAQA
                     GTSSSSNGGSSNGGAGGSGNGGAGGAGAAGATAAGGSGGNPAAGGSAAGGGASGGSAS
                     GGAAQGGSGGGGTATGEKFSFFMTSQAGLFKLAQAFNGSSKGFGGDLRYGKADGLSGA
                     DAICAALAETTMPKNGKTWRAFLSVTAGPDGKAVNAIDRIGEGPWYDRKGRLFAMNKA
                     ALLSERPTGIDPVIKDDFPNEEGVLNHDPGTGIIDNHNVLTGSTTMGAVDAQGKQSTC
                     QDWTSSESSGGKPRCGVSWPRASLKHWISVLAEGGCAPGSTPGNGAQSGPTGTVGALG
                     GYGAFYCFALTP"
     gene            1748..2176
                     /locus_tag="VFQ61_30515"
     CDS             1748..2176
                     /locus_tag="VFQ61_30515"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET9958879.1"
                     /translation="MKPSAARDEQLRELLTSRGLRVTTQRVALLRELSKVSSPISHPE
                     LTERMSRSGLDRATVYRNLLTLADAGVLVRAQLGDQVWRYELPRTLSSDHTHHPHLVC
                     SDCGVVRCLAAGTVKLHGEAAKSEVAEVQLRGRCVSCAAH"
     gene            2460..3368
                     /locus_tag="VFQ61_30520"
     CDS             2460..3368
                     /locus_tag="VFQ61_30520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958880.1"
                     /translation="MRQPLHSGRNERPEGVAPPGASIIATVRRSRPNCAFSKCAQLGA
                     VLALSANLLGCGQDSGTGGQRIGFELQVRSDLAPGEVYVTPRGWELRLTRAALALGPI
                     YIWRDPPPVAANPPVRRRAWQSLSDLLIPNAHAHAGDSHFAGGEVKGEYVAQFLFDAA
                     GEQPLPAFGPLRGSAGMAQSFSVVLDPPRAGTDDGRLQGHHAYVVGEARRGALTIPFR
                     GGMDIGREGTQRLIDGIQTELALSQGVQVELTVRVSRWFDEADFDLLETRGDDGVFEI
                     TDGSQAHTAWFIGLRSFDAFSMRLAP"
     gene            3495..4418
                     /locus_tag="VFQ61_30525"
     CDS             3495..4418
                     /locus_tag="VFQ61_30525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958881.1"
                     /translation="MFLRFEYGALRAALALSLSTFACACGSDSGALGEGALEVSVSGE
                     EAAEVGFPFTEDGETISFADGWRVSFSKLLIGVGGLDLRSSDGQTAVAESRTYVADLH
                     AGAARLATFSGLGARRWDRFSYRIVAPDETATPIGDVSEDDVERMREGGFNYLIEGSA
                     IREGNQLTFSWGFSNPTLNANCTNGVDDTDGVVVPNNSQASAEITIHVDHLFWTTLGT
                     ERSELRFEAIAAAGRDDGDISMSDLAEQRLSSLVGMDGEPLKDDQGAAIVYNPESVRL
                     ESQNLAAFMLASSSSMGHLNGEGLCTVRAKP"
     gene            4510..7131
                     /locus_tag="VFQ61_30530"
     CDS             4510..7131
                     /locus_tag="VFQ61_30530"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9958882.1"
                     /translation="MKALRWLCAVLALATEAHAQVIAPRIIEAPTAELPANVTQQARS
                     VPVLVVVAPDGSVTQAQVDAELEPQVAQLAIETARTYRFQPALRDGTPIAAKIRILVA
                     LVPAGAAAAPPSPEPSAKPEAQPSGDAAAPPPPARPPASPTQRGKAPAAPAPTAGESV
                     TDSSSAQDVVAPGGDAGTKVNDRAKEVVVTGRRDQKAPASASEYHVDPVQFADVPRHK
                     AEDLLTLAPGFVLANHGGELHPSTVYLRGFDAGEGQDIEFTVNGVPLNEVSNPHGHGY
                     SDTHFIIPELVEELHVVEGPFDARQGDFAVAGSVHYHLGLSARGLRATAGYGSFDSKR
                     LLLLWGPKGEQRGTFVGFEVGQGDGFGPNRAHALVRAMAGYERPLDVGTRLVLTATSY
                     AGRFDSAGVVREDDLRARRLPCPASEDQQFFCTYDPNQGGAASRHSISAGIEHEGSRH
                     GYQWLLFGTLRDLRLRENFTGFMTDFRASGEAQRGDGLEPQYSATTLGSRGHYAIYRE
                     AFGHRQRAEIGYFARHDRGSTSARRLRRDGGEPYKVVFDNGLRITNVGAYAMARVVPS
                     SRLVLRAGLRVDAFSFGVTDRNRPAQDRQGERLTRQTSEAFGFAFQPKASAEFLAFRD
                     WSVLASYGVGTRSSDAQALSDGEFAPFARVRAAEAGVVFRRPAPSEPSSEARLIAYST
                     HVDRDLIFDETTGRNVLSGASNRFGALFSGRVSSRVGLEAQGSVTYSEAYLSRAGSSA
                     FELKSGPRMPYIPRWVLRADTAFRRPLRVAEQPGEYAFGLGFTYVGPRPLPLNEYGHA
                     LASVDLSGRLRIRNAELGVQVTNLFDRRNRELEYNYVSNFQSAESLGSQFPEIHFSAA
                     PPRQIFGTLTLYWEDDS"
     gene            complement(7154..8548)
                     /locus_tag="VFQ61_30535"
     CDS             complement(7154..8548)
                     /locus_tag="VFQ61_30535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET9958883.1"
                     /translation="MWNLARANAGRARFGWLPVLVTSAAACASSVGSESSDKPGPENS
                     GAQGGSYTSAGASGGAAAGGTTSSAAGGSKVTPGSGGRASGGATAPASTSGGAAQGGG
                     SDAGGEAEQAGAPSEPLGGSPNSGGSGTGVAGGRTSPISFGPYKDTSIDLDWNTNIIS
                     TRISGAPAALATELSARGVRSVTLAFATGECGKENWAGVDGASMALGNASKLQNANVR
                     YILSTGGAAGAFTCSSDAGFATFLGRWQSPWLAGVDFDIEAGQSDAVISDLIARIPAA
                     RSANPELRFSLTLSTLANNAGGDQAQSLGAYVSDSLSTHGRAAIAAVRDVLAWKSPAT
                     WPGYLSVNLMVMDYGSVGSGVCVVRDGVCNMGESAVQAAHNLHDKWGVPYSNIELTPM
                     IGGNDVRDEHFTLQDVDRVVAFVKERGLAGVHYWSYDRDVDCPPGAASPTCNSLGGVG
                     AYGFLNRFLDAGLR"
     gene            8966..9532
                     /locus_tag="VFQ61_30540"
     CDS             8966..9532
                     /locus_tag="VFQ61_30540"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET9958884.1"
                     /translation="MQLEDACYTVLPLTQEMMMKKHFNSMILGLSICCGAYMALACGS
                     DDKDQPSSTGGSTATGGSTATGGAGTTGGSSNATGGSTATGGSTSTAEGEALYKSTGC
                     NGCHGEEAQGSLGPNITGSTTAGIGNWTSAEFLATLRTGKDDEGDQLCGTMPMYSATQ
                     LSDADVEAIRVFLMSKKNDTANPGSICN"
     gene            9535..10617
                     /locus_tag="VFQ61_30545"
     CDS             9535..10617
                     /locus_tag="VFQ61_30545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958885.1"
                     /translation="MSRLGLGAALLLLASVVAPMVSGCARLSASEGLPESELKPQAAP
                     RASARPDAPTYQALSSAPVVDRPLPQALPAPTAAPETIPFPYRPPGASARVYESSDPR
                     DQHRLVIGPGVTAGHSYSLLIGFHGQPRRGQAPREYAFGATVQSVVFPSTWAGRGSLE
                     SSTPSRPLILALPVFRFEGVNWPDFDVADFAEDVQRRLAERGIGVDRIFLFGHSGAAG
                     CGGAGLNRAADMRGTALDRGAVGFFDTCVGAGFTEAVRGLARRRVPTFIAHSVETAGF
                     RPRQRTEYLADFDFGRVYQPLGLRPTPCPERLPDAPLRPLTYRCAANPEDSARALVLD
                     TGHGEAAHQALLPVALRYFLERYVNR"
     gene            10689..11339
                     /gene="lepB"
                     /locus_tag="VFQ61_30550"
     CDS             10689..11339
                     /gene="lepB"
                     /locus_tag="VFQ61_30550"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9958886.1"
                     /translation="MKNASPSSALESRRKAIRRVRSTLSQWTGVLTALAGLSVARASL
                     ADHYVVPTGSMEPTVRIGDHILVNKAAYGLRVPFTSFWLSTVHAPDRGSVVVVDPPEP
                     GPVLLKRVIGLPGDLIEVRAGRLTINGNPVVVRRERAAELEMEYLGRPHEIRLDYAGG
                     PDFGPSRVPTGKLLVLGDNRGNSRDGRYFGYLDLAALRGRALAVYARSGSLTWQPL"
     gene            11705..13285
                     /gene="metG"
                     /locus_tag="VFQ61_30555"
     CDS             11705..13285
                     /gene="metG"
                     /locus_tag="VFQ61_30555"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET9958887.1"
                     /translation="MTTDQHSILYLSTTIPYVNAAPHVGFALELVQADALARYLRLTG
                     REVFTSSGTDDHSLKNLRSASAAGEPIRGFLDRHTREFESLAQLLGVHFDAFVSTSRD
                     PNHARSVQALLSACAANGDLYQRRYRGLYCVGCEQFYRPEELPQDLCPEHGEPLETIE
                     EDNWFFRLSRYRAELERAIETDRIRVLPTERKNEVLSFIQRGLQDFSVSRSAERAHGF
                     GLPLPGDSSQVVYVWVDALCNYLTAGAQRPRLLWRQAARRTHVLGKGVLRFHAVYWPA
                     LLASAGLPWPDELRVHGYLTVDGKKIGKSAGNGIDPRALIERFGKDALRYYLLKHTPA
                     FKDADFSCERLVSAHDSELADELGNLALRTLTLYARIPTPIPARALEIGEAEAPLEEC
                     ARGLAAQLEIAYSSHDFSSAGTLVWQLVRATNRYIDRNQPWRLLRSGTREDLAQAARV
                     LSISVAALRVLSGALDPLVPDLAGALARATGAERSSRALLDTSLLGHLSAPVPKQNPT
                     PLVPRLRERPARAPRRVE"
     gene            complement(13289..14845)
                     /locus_tag="VFQ61_30560"
     CDS             complement(13289..14845)
                     /locus_tag="VFQ61_30560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9958888.1"
                     /translation="MADVPSESHIGRVATRQLTLADFDRVVELQLTCFPSMEPWTREQ
                     FESQLRLFPEGQLCVTVDDLVVASSASLIVEFDLYSEWHDWRTLSGLGFIENHDPEGD
                     TLYGIEIMVHPEYRGLRLARRLYDARKELCRQHNLQRMVIGGRVPGFREHAKEMSASE
                     YVEHVQRRALYDPVLTAQLANGFVLRRLIPDYLPSDEDSAGFATHLEWLNLDYHSRGT
                     REFTQVQRVRVCAVQWQMRRITSFDEFSKQVAFFVDAASDYHADFVLFPELFTLELLS
                     LDHSIRPALAARKLSEFTPQYLELFTELAVRYHVNILGGSQFVLEEDRLFNVAYLFRR
                     DGTRAAQKKLHITPAETRWWGVEGGDAIDVFETDRGRIAINVCYDVEFPELARIVARR
                     GAQILFVPFNTDERHGYMRVRHCAQARAVENHLYVVIAGSTGNLPGVPNADIHYAQSA
                     ILVPCDFPFARDGVANECQPNIETLVLHDLDLELLRRHRSTGTTTNWGDRRRDLYELR
                     YLKRLKDGPI"
     gene            complement(15007..16830)
                     /locus_tag="VFQ61_30565"
     CDS             complement(15007..16830)
                     /locus_tag="VFQ61_30565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET9958889.1"
                     /translation="MILRKSVVLPVSFSLLSYAFGCSGGDLGGLGTIPGLGSGGTSGG
                     SSSSSGGANSPGSGNTMSSGAVNAGGSVSSSGNGNTGNASSSGSMSSGGGFTPGGGGN
                     GGTAPPPNGGVSSGTGGSGAGGGTFIPPTPPADAKPTPESSGIPGAFRVNTKTGHLMH
                     DGAEFQVRGGNWFGLEGQDDTARPGAMELYVGAVFWADASNKRTIDKTMQEIQSSALK
                     FNTIRLPVAPQTLVKGHPDGDYSRTDVKIRNNDPEIYPYAGSYEALEDFLLKASKNNL
                     YVILDLHSCSNHIGWRAGRVDDGPPWADSNRENYEYKKDNYYCNKGEDAYDKAKWLAD
                     IHTLAKLPKKLGIKNIIGIDCFNEPFKYSWSEWADLSKECYDAIAAEDDDLIAIVEGV
                     SGSHEVSPGVTEPEPYGDLTINPNWGENLYGQQIDPIQIPKDRLCFSPHTYGPSVFVQ
                     KQFVDQSDPACVGLEDDAAAKAKCKLVVDRKNAAATARIKQGWDEHFGYLRAQNYCVI
                     VGEFGGTKDWPKNPVEPAAATLWAHLPSGSRPDWEWQNMFVDYMKEKGITDFTYWSIN
                     PESGDTGGLYNHAYTESNKSGWGVWQGLDTEKVNMLAGINK"
BASE COUNT         3122 a         5656 c         5290 g         3155 t
ORIGIN      
        1 agtgtttccg ccgctcgagc tgccgccagt ggaagcgggt gtgctcggag catcggaaga
       61 acacgccgtc aacgagcccc aagcacaagc cgcgctcaac gcggtgaaga acaagacctt
      121 catggttcga gaggtttgcc agaaatccga gtgcctgggt agctcacggc gtcagtgcga
      181 agcagtagaa ggcaccgtat ccgcccagtg cccccacggt tccggtcggc cctgactgcg
      241 caccgttgcc gggagtggat ccgggcgcgc acccgccctc ggcgagaacg gagatccagt
      301 gcttgagcga cgcgcgcggc caggaaacac cgcagcgcgg cttgccaccg gagctctcac
      361 tgctcgtcca gtcctgacac gtagactgct tgccctgcgc gtcgactgcg cccatcgtcg
      421 tgctaccggt gagcacgttg tggttgtcga tgattccggt acctggatcg tggttgagca
      481 cgccctcctc gttcgggaaa tcatccttga tgacggggtc gatgccggtg ggtcgctcgc
      541 tcagtagagc cgccttgttc atcgcgaaca gccgcccttt acggtcgtac cagggcccct
      601 cgccaatgcg atcgatggcg ttgacggcct taccatcggg cccggcggtt acactcaaga
      661 acgcacgcca ggtctttccg ttcttgggca tcgtggtctc cgcgagcgcg gcacagatcg
      721 cgtccgcacc gctcaagcca tctgctttgc cgtagcgtag gtcgccccca aaccctttgg
      781 agctgccgtt gaaggcttgc gcgagtttga acaagccagc ttggctcgtc atgaagaagc
      841 tgaatttctc gccggtcgcg gtgcctccac cgccggatcc gccttgagct gcaccgccgg
      901 acgcggaacc accgctcgca ccaccgccag ccgcactacc tccagcggca ggattgccgc
      961 ccgagcctcc agcggcagtt gcacctgcgg ctcccgcgcc gcccgcgcca ccgttgcccg
     1021 agccacccgc ccctccgtta ctcgacccgc cattagaact agaagaagtg ccggcctgtg
     1081 ccgtcgagcc ccccatcgga ccattggatc gcccaccact ggcagctcca ccagacgcgc
     1141 caaccgtgct cgctccgccc gaagtcgaag caccaccgct agccgcggga gccccagcgc
     1201 tcgtgcctcc tgtctgagct gacccggcgc cgctcccacc ttgggaggat gaactcccac
     1261 ccttcccagc actcggctcg tcgctaccac cgcaccccag catcaaaatg gctggaaaag
     1321 cagcgaggca cagcgtgagg gaaagatgcg acaccatatc gtgaccgaat cgtttcatag
     1381 gattggacaa acctgtggtt gggcgcggcg cgggtcttga ttggactcag taaccgctgc
     1441 ctccgtaaat gaggcgagtg cattcacgat cgcgcccgag tgtcgtcgaa gcgttccatg
     1501 tcctcgccaa atttcgtggc tcggcgagag caacctccag agtgcatagg ttgggtgacc
     1561 gattcggaga cagcgctgac gcgaagccga ccaacgacat tacgcctgct cagtttttgc
     1621 aacccactcg caaaatcgtc gacagccctt gagcgaaaag agtcgcttcc cgagccagga
     1681 tcattcctcg agaaatcgaa gcaaccaggt tgcggagcgc gcggcagcgc gggattcttg
     1741 ggtcgccatg aaaccctccg ctgctcgaga cgaacagctc cgtgaacttt tgacctcgcg
     1801 tggattgcgg gtgacaacgc agcgtgtggc tttgcttcgc gaactctcga aggtgagctc
     1861 acccatcagc caccccgagc tcaccgaacg catgtcgcgc agcggcttgg atcgcgccac
     1921 ggtctatcgg aacctgctca ccctggctga cgccggggtc ttggtgcgcg cccagctcgg
     1981 ggatcaagtc tggcgttatg aactgcctcg aacgctgagc tccgatcaca cccaccaccc
     2041 gcatttggtc tgctcggact gtggcgtcgt gcgttgcctg gccgctggca ccgtcaagct
     2101 gcacggcgaa gccgccaaga gcgaagtggc cgaggtgcag ctgcgcgggc gctgcgtgag
     2161 ctgcgcggct cattgacctc cgggtcgagg gccccggcga ctgcttagtg cgctttagtt
     2221 gcatctaagc ggctcccccc gaaagcctgc cgtgccccga attcctccgt tacgcctcgt
     2281 gacggacccg gcccaaacgc gaatgctttc gaagatagaa tgccctcttc ctcccgtttc
     2341 gagaggccaa gagagacgct ccgagccccg ctcggtcgaa cgcagaagcg aacggagcgg
     2401 tcgtccgaag aaagaactca gttgcatctg ggaccaccgt gatctacatg ctgcgcgaca
     2461 tgcggcagcc tctgcacagc ggtcggaacg aacgccccga aggcgttgcg ccccccggcg
     2521 cctcgatcat cgcgacggtg cgccgctcga ggccgaattg cgcgttctcg aagtgtgccc
     2581 agctcggcgc ggtgctcgcg ttgtccgcga acttgctggg ctgcgggcaa gatagcggca
     2641 ccggagggca acgtatcggt ttcgagttgc aggtgcgttc cgacttggca cccggagaag
     2701 tctacgtcac tccgcggggt tgggaacttc gactgacccg ggccgccttg gcgctgggtc
     2761 caatctacat ctggcgcgat cctccgcccg tcgccgccaa tcccccggtg cggcgccgcg
     2821 cgtggcaatc tctgtccgat ttgctgatcc cgaacgcgca tgctcacgct ggcgacagcc
     2881 actttgccgg cggcgaggtc aagggcgaat acgtcgcgca atttctgttc gacgccgcag
     2941 gcgaacaacc gttacccgcg tttggtccct tgcgtggctc cgcgggcatg gcccaatcgt
     3001 tcagcgtcgt actcgatccg cctcgcgccg gaacggacga cggtcggctg caggggcacc
     3061 acgcctacgt cgtgggagaa gcaaggcggg gagcgctgac aattccattc cggggcggca
     3121 tggacatcgg tcgcgagggc acccagcgtt tgatcgacgg gatccaaacc gagctcgccc
     3181 tatcccaagg cgttcaggtc gagctcacgg tgcgtgtgag tcgctggttc gatgaggctg
     3241 atttcgatct gctcgagacg cgcggcgatg acggcgtatt cgaaatcacc gatggtagcc
     3301 aagcgcacac ggcctggttc atcggtctgc gtagcttcga cgcattctcg atgcgactcg
     3361 cgccgtgaac ccgtgaacgc gcggagcccg cgaacgaaat caacaccgga acgtgcggag
     3421 cccgggaacg aatcgatacc ttgaacgctt gccgcgacag tttggctcaa ttcagcacct
     3481 agaggagaag tccgatgttt ctgcgattcg agtacggcgc gttgcgcgct gctttggccc
     3541 tatccttgtc tactttcgcc tgcgcttgtg gctccgactc tggagcgctc ggagagggag
     3601 cgctcgaagt cagtgtttcc ggagaggagg cggccgaagt cgggtttccc tttaccgaag
     3661 acggggaaac gatttctttt gccgacggct ggcgcgtaag cttctccaaa ctcctgatcg
     3721 gggtgggcgg tctcgactta cgcagctccg acgggcagac cgcggttgcg gagagccgaa
     3781 cgtatgtcgc ggatctgcac gcgggcgcag ctcgtttggc cacgttttca ggcctcggcg
     3841 cgcgacgctg ggatcggttc agttatcgaa tcgtcgcccc ggacgagacc gcgacgccga
     3901 ttggcgacgt gagcgaagat gacgtcgagc gcatgcggga gggcggattc aactacttga
     3961 tcgagggcag cgccattcgc gagggcaacc agctaacttt cagctggggc ttctcgaatc
     4021 ccacgctgaa cgcgaactgc acgaacggcg tcgacgacac ggacggagta gtggtgccga
     4081 acaatagtca ggcctccgcc gagatcacga tccatgtcga tcacctgttt tggaccaccc
     4141 tcgggacgga acggtccgag ctacggttcg aggccatcgc cgccgcaggc cgcgatgacg
     4201 gtgacatcag catgagtgac ctcgccgagc aacggctgtc gagcctggtc ggtatggacg
     4261 gtgagcccct gaaagacgac caaggtgcgg ccatcgttta caacccggaa agcgtgcgcc
     4321 tcgagtcgca gaatctcgca gcgttcatgc tcgcctcctc gagttctatg ggccacctca
     4381 acggagaggg actctgtacc gtgcgcgcca aaccatgacc ggctgcttcc gctcacgacg
     4441 catcgggcag gcgtgttcac cagcttcccc tgctcgcact ctttcgtggt aggcgtgccc
     4501 catcgaacga tgaaagcact ccgctggctc tgcgccgtgc tggccctcgc caccgaggct
     4561 catgcccaag tcattgcacc acgcatcatc gaggcaccca ccgcggagct gccggccaat
     4621 gtgacccagc aagcgcgctc cgtgccggtc ctcgtggtgg tggcaccaga cgggagcgtg
     4681 acgcaagcgc aggtcgacgc agagttagag ccccaagtcg cccaactagc gatcgagacg
     4741 gcgcgcacgt atcggttcca gcccgcgctg cgcgatggga cccccatcgc ggccaagatc
     4801 cgcatcctgg tcgcgttagt tcccgcgggt gcggcggcag cgccgccgtc gcctgaaccc
     4861 tcggcgaagc cggaagcgca gcccagtggc gacgcggcgg ctcctcctcc gcccgctcgc
     4921 cctcctgcgt cgcccacgca aagggggaaa gccccagctg ctccagcgcc aacggcgggc
     4981 gagtccgtca cagactcgag ctccgctcag gacgtcgttg cgcccggcgg cgacgcgggc
     5041 accaaggtca acgatcgcgc caaagaggtc gtggtgaccg gtagacgcga tcagaaggca
     5101 cccgcctccg cgagcgagta tcacgtcgat ccagttcagt tcgcggacgt gcctcgccac
     5161 aaggccgagg acttgttgac gctcgcgccc ggcttcgtgc tcgcgaatca cggcggggag
     5221 ctccaccctt ctaccgtcta cttgcgcgga ttcgacgccg gtgaaggcca agatatcgag
     5281 tttacggtca acggcgtgcc gctgaacgaa gtttccaatc cccacgggca cggttactcg
     5341 gatacacact tcatcatccc cgagctcgtg gaagagctgc acgtcgtcga gggcccgttc
     5401 gacgcgcgcc aaggcgactt cgcggttgcg ggcagcgttc actaccacct tgggctctcc
     5461 gcgcgcgggc tccgcgctac ggccggctac ggctcgttcg attcgaagcg tctgctgctg
     5521 ctttgggggc ccaagggtga acagcgcggc accttcgttg gcttcgaagt cggtcagggc
     5581 gatggcttcg gccccaatcg cgcccacgcc ttggtccggg ccatggcggg atacgagcga
     5641 ccgctcgacg tagggacccg cttggtgctc accgcgacca gctatgccgg tcgcttcgac
     5701 agcgcgggcg tggtccgtga agacgacttg cgcgcgcgcc gattgccctg ccctgcctcc
     5761 gaagatcagc aattcttctg cacctacgat ccgaatcaag gcggggccgc ttcgcgtcac
     5821 agcatctccg cggggatcga gcacgagggc agccgacacg gctatcagtg gctgctattc
     5881 gggaccctac gggacctgcg cctgcgcgag aacttcaccg gcttcatgac agacttccgc
     5941 gcgagcggcg aggcgcaacg tggggatggt ctcgagccgc agtacagcgc aaccacgctc
     6001 ggttcccgtg gacattacgc gatataccgg gaggctttcg gccaccgtca gcgcgcggaa
     6061 ataggatact tcgcacgcca tgaccggggc agcacctcgg ctcggcgatt acggcgcgac
     6121 ggcggcgagc cgtacaaggt cgtgttcgac aacggcctgc gcatcaccaa cgtcggcgct
     6181 tacgcgatgg ctcgcgtggt ccccagctca cgcttggtat tgcgggcggg cttgcgcgtg
     6241 gacgcattct ccttcggagt caccgatcgc aatcgtcccg cccaagaccg ccaaggcgag
     6301 cgcctcacgc gacaaacctc cgaagcgttt gggttcgctt tccaacccaa agcctccgcc
     6361 gaatttctgg cattccgaga ctggtccgtg ttggccagct atggcgtggg gacccgctcg
     6421 agcgatgcgc aggcgctttc tgacggcgag tttgcgcctt tcgctcgcgt gcgggccgcc
     6481 gaggccggcg tggtgttcag gcgtcccgct ccgagtgaac cctcgtccga agcgcgcctg
     6541 atcgcgtact cgacccacgt cgatcgcgac ctgatcttcg acgagactac gggccgcaac
     6601 gtactctcgg gggcttccaa tcgtttcggg gccttgttct cgggacgtgt gagctcccgc
     6661 gtcggcctgg aagcccaagg cagcgtaacg tactccgaag cttacttatc gcgcgctgga
     6721 agctcagcat tcgagctgaa gtcggggccg cgcatgccct acatcccgcg ttgggtgctg
     6781 cgcgcggata ccgcatttcg tcgaccgctg cgcgtcgcgg agcaacctgg cgaatatgcg
     6841 tttggactcg ggtttactta cgttggcccc cgaccgttac ctctgaatga gtacggccac
     6901 gccttggcga gcgtggacct gtcgggtcga cttcgaatcc gcaatgcgga gctcggtgtg
     6961 caagtcacca acttgttcga ccgtcgcaat cgcgagctgg agtacaacta cgtctcgaac
     7021 ttccagagcg cagagagcct gggttcgcag tttcccgaaa ttcacttcag cgccgccccg
     7081 ccccggcaaa tcttcgggac cttgacgttg tattgggaag acgacagctg atttggtcga
     7141 gcaccacccg ccgtcagcgc aatccggcat ccaggaagcg gttcaggaag ccgtacgcac
     7201 ccacgccacc tagcgagttg caggttggtg acgcggcccc cggcggacag tcgacatcac
     7261 gatcatagga ccaatagtgc acaccggcca agcctcgttc tttgacgaag gccacgaccc
     7321 gatcgacgtc ctgcagcgtg aagtgctcgt cccgcacgtc attgccgccg atcatcggcg
     7381 tcagctcgat gttcgagtac ggcacgcccc acttgtcgtg caggttgtgg gcggcttgca
     7441 cggccgactc acccatgttg cagacgccgt cccgcaccac acagacgccg gaaccgacgc
     7501 ttccgtaatc catgaccatc aaattcacgc tcaaatatcc tggccatgtc gccggtgatt
     7561 tccaggccag cacgtcccgc accgcggcta tcgccgcccg cccgtgcgtg ctcaagctat
     7621 cggatacgta agcaccgagt gactgggctt ggtcgccgcc cgcgttgttg gccaatgtcg
     7681 agagcgtgag gctgaaccgc agctcggggt ttgcgctgcg cgcggcggga atgcgcgcaa
     7741 tcaagtcgga gatcaccgca tccgattgac ccgcctcgat gtcgaagtcc acgcccgcga
     7801 gccacggaga ctgccaacgc cccaagaacg tggcgaagcc cgcgtccgag gagcacgtga
     7861 atgcccccgc agcgccgccg gtcgaaagaa tatatctgac gttggcgttc tgtaacttgg
     7921 atgcgttgcc aagggccata gaggcgccgt cgacgccggc ccagttctcc ttgccgcact
     7981 ctccagtcgc gaacgccaag gtcacgctgc gcacaccgcg ggccgagagc tccgttgcga
     8041 gcgcagccgg tgcccccgag attcgcgtcg agatgatgtt cgtattccaa tcgagatcga
     8101 tactcgtatc cttgtatggg ccgaacgaga taggcgaagt tcgccctccc gcgacgcccg
     8161 taccactgcc gccggagttg ggtgagccac ccaacggctc gctcggcgcg cccgcctgtt
     8221 cggcctcgcc gccagcatca ctgccacccc cttgggccgc gccgccactg gtcgaagcgg
     8281 gagcggtcgc cccgccgctc gcacgaccac cactcccagg ggtaacctta cttccacccg
     8341 cggcactcga ggttgtacca cctgcggcgg cgcccccgct ggcaccggct gacgtgtagc
     8401 ttccgccctg cgcacccgaa ttctcgggcc ccggcttgtc gctggattcg ctacccacgc
     8461 tactggcgca agcagcagcg ctcgtaacga gcaccggcag ccagccaaaa cgcgcgcgac
     8521 ctgcgttggc gcgagccagg ttccacataa gatatccttg tcgcgctgcg cgcacgacgg
     8581 gcgggacgag aggaaggaaa gaacgagggg gagccaagca gcaaagccgg ccacgggggc
     8641 cgaggttgag tatttacgcg cacacggcga atgcaaaccc gttacagacg tcgacgagcc
     8701 acggcgggca ccgtttggct agcctcgagt catgcgggac gcgtgatccg cacgttttcc
     8761 cggagtttcg gagtcgttgc cgtaggtgac ccaaggctgg agccgcgcat ggcacgctcc
     8821 aaaaattgtc cgttgtaaag ggcagtgctg gcttctggca tcatcgacct cgaccgcgcg
     8881 gagcgagttc cccacggttg cgttcgagac gtgctccgcg tgtcctacac cagcgccagt
     8941 tcgatcgatg atccgtaggt gacacatgca actcgaagat gcgtgctata cggtcctccc
     9001 gctcacacaa gagatgatga tgaaaaagca tttcaattcg atgattctgg gattgagcat
     9061 ctgttgcggc gcctacatgg cacttgcgtg cggcagcgac gataaggacc aacccagcag
     9121 caccggcggc tcgacggcta cgggcggctc gactgccacg ggtggcgcgg gaaccacggg
     9181 tggaagcagc aacgctaccg gcgggagcac ggccacgggt ggatcgacca gcactgccga
     9241 gggtgaggcg ctgtacaaat cgaccggttg taacgggtgc cacggcgagg aagcccaagg
     9301 ctcactcggt ccgaacatca ccggttccac gacggcaggc attggcaact ggacgtccgc
     9361 ggagtttctc gccaccctac gcacgggtaa ggacgacgag ggcgaccagc tgtgcgggac
     9421 catgcccatg tacagcgcga ctcagctcag cgacgcggac gtcgaagcca ttcgcgtgtt
     9481 cctgatgagc aagaagaacg acactgccaa tcccggcagc atctgcaatt gagcgtgagc
     9541 cggcttggac tcggcgcagc actcctgctc ctcgcgagcg tcgtcgcccc gatggtttcg
     9601 ggttgcgctc gtctctccgc gagcgagggg ctgcccgagt ccgagctgaa accgcaagcg
     9661 gcgccccgag cttcggcgcg acctgacgca cccacgtacc aagcgctctc gagcgcaccc
     9721 gtcgtagatc ggccgcttcc gcaagccctc cctgcaccga cggcggcacc cgaaactatc
     9781 ccattcccgt atcgcccacc cggggccagt gcgcgcgttt atgagtcttc ggacccgcgc
     9841 gaccagcatc ggttggtgat tggtccgggt gttaccgcgg gccatagcta ttcgctgtta
     9901 atcggcttcc atggccagcc tcggcgaggt caggcgcctc gcgagtatgc gtttggagcc
     9961 acggttcaaa gcgtggtttt cccgagtact tgggccgggc gcggatccct cgagagctca
    10021 acgccctcga gaccgttgat cctcgcgttg ccggtgttcc gcttcgaggg tgtgaactgg
    10081 cctgacttcg acgtcgcgga cttcgcagaa gacgtacaac gcaggctggc cgagcgggga
    10141 atcggagtcg accgcatctt cctattcggg cactcgggag cggccggctg tggcggagca
    10201 gggctaaatc gtgccgctga catgagaggc acggcgctcg ataggggcgc ggtcggcttc
    10261 ttcgacactt gcgtgggcgc cggcttcacc gaagccgtgc gtgggctcgc gcgccggcgc
    10321 gtgcccacct tcattgcgca cagcgtagag accgcaggtt ttcggccgcg ccagcgcacc
    10381 gagtacctcg ctgacttcga tttcgggcgt gtctatcaac cgctcggtct gcggcccacc
    10441 ccttgtccgg aacgcctgcc cgacgcgccg ttgcgccccc taacctatcg atgcgccgcc
    10501 aatcccgaag acagcgcccg cgcgttggtc ctcgacaccg gacacggcga agccgcccac
    10561 caagcgctat tgccggtcgc gctacgctat tttctcgagc gatacgtgaa ccgctgatcg
    10621 cgcctggcga gacgctagaa gccgcgtacc cttagagctg cactcaggga caacggagct
    10681 tgcccagcat gaagaacgcg tcgccatcta gtgctctgga atcgaggaga aaggccatcc
    10741 gccgcgtgcg cagcaccttg agccaatgga cgggagtgct caccgcattg gcgggcctct
    10801 cggtagcccg cgcgtccttg gcggatcact atgtagtgcc cacgggctcc atggagccca
    10861 ccgtacgaat tggagaccac attttggtca acaaggccgc ctacggcctg cgtgtaccct
    10921 tcacgagctt ttggctcagc accgtgcacg cgccggacag aggcagcgta gtcgtcgtcg
    10981 atccgccgga gccgggtccg gtgcttctca agcgggtgat cggtttacct ggtgacctta
    11041 tcgaggtacg agccgggcgc ctgacgatca acggtaatcc ggtggtcgtg cgccgagagc
    11101 gagcggcgga gctcgaaatg gagtatctgg ggcgcccaca cgagattcgg ctggactacg
    11161 cgggcggccc cgatttcggc ccgagccgag tgcccactgg caagctcttg gtgttgggcg
    11221 acaaccgcgg gaacagccga gatggtcgtt acttcggtta cctggacttg gctgcgctgc
    11281 gcggccgggc cctcgccgtg tacgcgcgca gtggttcact cacctggcag ccactctagt
    11341 gctttcgcca gaggcccctc tgagacgatc gacgtccatc gaaagttgaa atcgtgtgtc
    11401 agcggtacgg gtttgacatt gggctcggct gacgtcatgt tttgcgcgct gtgaaacaac
    11461 tccacatttg caacaatcgc gcggaaaaca tgcgcgctcg gctgacattc ggccgggcgc
    11521 agaagatcgc cgcgtaacga gctgtttcac agccgggtcc gagccacgct cgaatccgta
    11581 gaatcgcgac gggctgcccc acggcagccc gttctgtttt gtttgctctc ggtacgtggc
    11641 tgcatccgtg gaggtggccc tcgccaaacc gtcgatcctg cgcggtcgac acgacgaggc
    11701 aaccatgacc accgaccagc actcgattct ttacctctcg acgactattc cctacgtcaa
    11761 cgccgcgccc cacgtcggct tcgcactgga gctcgtgcaa gcggatgcgc tcgctcgcta
    11821 ccttcgcctc accgggcgcg aggtgttcac ttcgtcgggc acggacgacc acagcctgaa
    11881 gaatctgcgg tctgcgagcg ctgcgggcga accgatccga ggctttctgg atcgtcacac
    11941 gcgagagttc gagtcactcg cgcaattact cggcgttcac ttcgacgctt tcgtttccac
    12001 gagtcgagac ccgaaccacg cccgcagcgt ccaagctctc ctgagcgctt gcgcggcaaa
    12061 cggtgacctc tatcagcgac gctaccgcgg actctactgc gtcggctgcg agcaattcta
    12121 tcgaccagag gagctaccgc aggacctctg tcccgaacac ggggagccgc tagaaacaat
    12181 cgaagaagac aattggttct ttagactctc tcgctatcga gccgagctcg aacgagccat
    12241 cgagacggac cgcatccgag tattgccaac cgagcgcaag aacgaggtac tgagtttcat
    12301 tcaacgcggt ctgcaggact tcagcgtgtc tcgcagcgcc gagcgggcgc acggattcgg
    12361 cctacccctg cccggtgaca gctctcaggt cgtctacgtc tgggtcgacg cgctgtgcaa
    12421 ttacctcacg gccggcgccc agcggccacg gctactttgg cggcaagccg ctcgccgcac
    12481 ccatgtactc ggcaagggcg ttctacgctt tcacgccgtg tattggccgg cactgctggc
    12541 ttccgccggt ttgccctggc ccgatgaact gcgcgtccat ggctatctaa ccgtagacgg
    12601 caagaagatc ggtaagtccg ccggcaatgg gatcgatccg cgcgcgctaa tcgagcgctt
    12661 cggcaaagat gcgctccgat attacctctt gaaacacact cccgccttca aagatgcgga
    12721 cttttcctgc gagcgattgg tctccgccca cgacagcgaa cttgccgacg agctcggcaa
    12781 cttggcgttg cgcactctta ccctgtacgc caggattccc actcccatcc ctgcccgagc
    12841 tctcgagatt ggcgaagccg aggccccgct cgaggagtgc gcccgcggtt tggccgctca
    12901 gctcgagatc gcgtactcga gtcatgactt cagctcggcg ggcacgctgg tttggcagct
    12961 cgtgcgcgct acgaaccgct acatcgaccg gaaccagccc tggcgactgc tccgcagtgg
    13021 aacgcgcgaa gacctcgccc aagccgctcg agtgttatcg atttccgtgg cagccctgcg
    13081 cgttttatcc ggcgcactcg acccgctggt ccctgacttg gccggcgcac tcgcgcgagc
    13141 gaccggcgcg gagcgaagta gccgagcgct gctcgatacc agcctgctgg ggcacctgag
    13201 cgcgccggtg ccgaagcaga accccacgcc gctggtccca cgcctgcgtg aacgccctgc
    13261 gcgcgcgcca cggcgcgtcg aatgatggtc aaatcgggcc gtctttcagg cgtttgaggt
    13321 aacgcaactc gtacaagtcg cgccgccggt ccccccagtt cgtggtggtg ccggtcgagc
    13381 gatggcgtcg cagcagttcc aaatccaagt cgtgcaacac gagagtctcg atattgggtt
    13441 ggcattcgtt ggcgacgccg tcgcgcgcga acgggaaatc gcagggcacc aagatggcgg
    13501 actgcgcata atgaatgtcc gcgttcggaa ccccgggcag attgccggtg gagccggcga
    13561 tcaccacgta caagtgattc tcgaccgcgc gagcttgcgc gcagtgccgc actcgcatgt
    13621 agccgtggcg ttcatcggta ttgaacggta cgaacagaat ctgtgcgccc cggcgcgcga
    13681 cgatacgcgc caactccggg aactccacgt cgtaacaaac attgatcgcg atccgcccgc
    13741 ggtccgtctc gaagacgtca atcgcgtcgc ccccctccac cccccaccag cgggtctccg
    13801 caggcgtgat atgcagcttc ttttgcgcgg cgcgcgtgcc gtctctccgg aacaggtacg
    13861 ccacgttgaa caagcgatct tcttccaaga cgaactggct accacccagg atgttgacgt
    13921 ggtagcgcac cgcgagctcg gtgaacaact ccagatactg gggcgtgaac tcgctgagct
    13981 tgcgggccgc cagcgcgggc cgaatcgagt gatccaggga gagcagctcc agcgtgaaca
    14041 actccggaaa gagcacgaag tcggcgtgat agtcgctcgc cgcgtcgacg aagaaggcca
    14101 cttgcttcga aaactcgtcg aagctcgtga tgcgccgcat ttgccactgt acggcgcaca
    14161 cgcgaacacg ctgcacttgg gtgaactcgc gcgttcctcg tgagtggtag tcgagattca
    14221 gccactcgag gtgtgtcgcg aaacccgccg agtcctcgtc cgacgggaga tagtctggga
    14281 tcaggcgcct gagcacgaag ccattggcga gctgggccgt gagcacggga tcgtacaagg
    14341 cgcggcgttg cacgtgctcc acgtactcgc tggccgacat ctctttggca tgctcacgaa
    14401 agccgggcac gcggccgcca atcaccatgc gctgcaggtt gtgctggcga cacagctcct
    14461 tgcgcgcatc gtacagccgc cgagccagac gcaagccccg atactcgggg tggaccatga
    14521 tctcgattcc gtagagcgtg tccccctccg gatcgtgatt ttcgatgaac ccgagtccgg
    14581 aaagcgtgcg ccaatcgtgc cactcgctat acagatcgaa ctcgacgatc aggctcgcgg
    14641 aggaggcgac caccaggtcg tccaccgtga cgcagagttg cccctcaggg aagagccgga
    14701 gctggctctc gaactgctct cgcgtccacg gctccatgct cggaaagcac gtgagctgca
    14761 gctcgaccac ccgatcgaag tcggccagcg tgagctgtcg cgtcgccacg cgtccgatgt
    14821 gagattcgga gggaacgtcg gccatgagtg ttcatagccc gagcgacagc cagatgttag
    14881 aggcgccccg tggcgattgg gtgaaccgaa cggcgggagc ttgcacccgc tccggtacga
    14941 acagccggcg aggggcgcgc tacatccgcc tgccctattc ggggcaggcg gatcgcgacg
    15001 agataactac ttattgatcc cggccagcat gttcaccttt tcggtatcca gcccttgcca
    15061 cacgccccag ccggacttgt tcgattcggt ataggcgtgg ttgtagaggc cgcccgtgtc
    15121 acccgactcg gggttgatgg accagtacgt gaagtcggtg atgcccttct ccttcatgta
    15181 gtccacgaac atgttctgcc actcccagtc cgggcgcgat ccggaaggca ggtgggccca
    15241 aagcgtggcg gcagccggtt cgaccgggtt cttcggccaa tccttggtgc caccgaactc
    15301 cccgacgatg acgcagtagt tctgagcgcg cagatagccg aagtgctcgt cccaaccttg
    15361 cttgattctg gcggtagcgg ccgcgttctt tcgatccacc accagcttgc acttggcctt
    15421 ggcggccgcg tcatcctcga gcccgacgca cgcgggatcg ctctgatcga cgaattgctt
    15481 ctgaacgaac accgacggac cataggtgtg gggcgagaag cacaagcggt ccttcggaat
    15541 ttgaattggg tcaatctgct gcccgtacag gttttcaccc cagttcgggt tgatggtcag
    15601 atccccgtaa ggttcgggct ccgtcacacc gggcgagacc tcgtgactgc cagacacccc
    15661 ctcgacgatg gcgatcaagt cgtcgtcctc ggcggcaatc gcgtcgtaac attccttcga
    15721 aaggtcggcc cactcggacc aagagtactt gaaaggctca ttgaagcagt cgatgccgat
    15781 gatgttcttg atcccgagct tcttcggcaa tttggcgagc gtgtggatgt ccgccaacca
    15841 cttggccttg tcgtaggcgt cttcaccctt gttgcagtag tagttgtctt tcttgtactc
    15901 gtagttctcg cggttgctgt cggcccaggg cgggccgtcg tccaccctgc ccgcgcgcca
    15961 accgatgtgg ttcgaacagg agtgcagatc gagaatcacg tacagattgt tcttgctggc
    16021 cttgagcaag aaatcttcga gcgcttcgta gctacccgca tacggatata tctcgggatc
    16081 gttgttccgg atcttcacgt ccgttcgtga atagtcacca tccggatggc ccttgaccag
    16141 ggtctgtggg gcaactggca aacgaatcgt gttgaacttc agcgcagaag actgaatctc
    16201 ctgcatggtc ttgtcgatcg tgcgtttgtt ggaggcgtcg gcccagaaca ccgcgccgac
    16261 atagagctcc atcgctccgg ggcgagctgt atcgtcctgc ccttccagcc cgaaccagtt
    16321 gccaccacgc acctggaatt ccgcaccgtc gtgcatcaga tggcctgtct tggtgttgac
    16381 gcggaacgcg ccgggaatac cggagctctc gggtgtgggc ttggcatctg ccggcggggt
    16441 tggggggatg aaagtaccgc cgcccgcgcc gctgccgccg gtcccgctgc tcacgccacc
    16501 gttcggtggc ggggcagttc ctccattgcc accacccccc ggagtgaaac cgccgccaga
    16561 gctcatcgag ccgctcgagg aggcgttccc ggtgttgcca ttgccggagg atgacacgct
    16621 gccccccgcg ttcaccgccc ccgaagacat cgtattgcca ctaccggggc tgttcgcacc
    16681 accactgctg gagctgctgc cacccgaggt gccaccgctg cccaagccgg ggatcgtacc
    16741 caaaccgcct aaatccccac cggagcagcc gaatgcatag ctcaggaggg aaaaggaaac
    16801 cgggaggacg acgcttttcc gcaatatcat gtgttctctc tttccatcgg tacgaattca
    16861 gtgcagagct gcgctgatga tggggtggct tcgccggcaa tccgagatag ccgacctggt
    16921 cgatgaggtt ggctacgctg gttcatgagg ttggctacgc tggcccatgg gttagccgag
    16981 ctggtgagtg cgccagctcc gcctcccgcc agcgaatcgg ctgggacagt gcaaaatctc
    17041 agggtctcgg caatcctcac gacccggcga atactgggcc ccaatacgac tgttcgtaag
    17101 ccgccgtagg cgataagcac catgcaggag ccctgatcgg agcgcctcgg ctcgggtcgg
    17161 gcctgcacgg aattcgtaaa tacgcaacac caatgggttg cgaactcggc accttcatta
    17221 acc
//
LOCUS       DASTKU010000867         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1732219, whole genome shotgun sequence.
ACCESSION   DASTKU010000867 DASTKU010000000
VERSION     DASTKU010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732219"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1785
                     /locus_tag="VFQ61_30570"
     CDS             <1..1785
                     /locus_tag="VFQ61_30570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9958890.1"
                     /translation="MPKGHLPVRSYLAVPVKSRSGEVLGGLFFGHHERGVFRKEDELT
                     LDAVSAHAAIALDNAKLYQAARQAEAQAQAEREKLRQLFLQSPALIIILKGPDHLFEF
                     VNASAYKLLGGVEVIGKPLSEAIPEFPAERRKLLDRVFLTGERYAEEAMSSTRDWQRN
                     GRPHERFFNMVYEPYRGTDGRVEGLMAFGFEVTDQVLANRKIERVVQELEVANRAKDE
                     FLAVVSHELRTPLNAILGWTRMLRSSSFSEEKRSRALETIERNANAQAQLIEDLLDVS
                     RIISGKLRLDVASVDVASVLDEAIDVVRPAAYAKGIRLKQTLDPNAGPILGDPNRLKQ
                     VIWNLLTNAVKFTPKGGSVHIALRKRDSSIEIVVSDSGQGIEPEFLEHVFERFRQADA
                     TTTRKHGGLGLGLAIVRSLVELHGGTVRVTSEGLGKGSSFIVCLPISPLRGAEIESPP
                     AVRLVMKSSLQPSTELANVHVLVVDDEDDARDLVAEMLISAGAEVSTAASAREALGFV
                     RERRPDLVVSDIGMPGEDGYSFIKQLRELPASQGGRTPAIALTAYARVEDRTKALLAG
                     FNMHVTKPVEPRELIALLSSLAALFERK"
     gene            1946..>2389
                     /locus_tag="VFQ61_30575"
     CDS             1946..>2389
                     /locus_tag="VFQ61_30575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HET9958891.1"
                     /translation="MLSELAIILVLVLANGFFSGAEIAVVALRRGRIKELAEQGRGSA
                     RAVLALRSQPERFLATVQVGITVVGATAAAFGGAAIAERLAPLLARVEWLAHHAEGLA
                     LGIVVSVVSFLSIVVGELVPKSLALRNAERFALLIGKPLLWLSWLA"
BASE COUNT          437 a          728 c          784 g          440 t
ORIGIN      
        1 atgcccaaag gacatctccc cgtccggagt tacctcgcgg taccagtcaa gtcacgctct
       61 ggcgaggtcc tcggaggatt gttcttcggg caccacgagc gcggcgtgtt ccgcaaggaa
      121 gacgagctta cgctcgacgc cgtgagtgcc cacgccgcca tcgcgctcga taacgccaag
      181 ctgtaccagg ccgcgcgcca agcggaagcc caagcccaag ccgagcgcga gaagctgcgt
      241 cagttgttct tgcagtcacc cgcgctcatc ataattttga aggggccaga ccacctcttc
      301 gagttcgtca acgcatctgc ttacaagttg ctcggcggag tcgaggtgat tggtaagccc
      361 ttgagcgaag cgatacccga gtttccggcc gagcgccgca agctcttgga ccgcgtattt
      421 ctgaccggcg aacgctacgc ggaagaggcc atgagctcga ctcgggattg gcagcgcaac
      481 ggacgccctc acgagcgctt cttcaacatg gtttacgagc cttaccgggg cacggacggg
      541 cgcgtagagg ggctgatggc atttggattc gaagtcacgg atcaagtgct cgccaatcgc
      601 aagatcgagc gcgtcgtgca ggagctcgag gtcgccaatc gcgctaaaga cgagttcctc
      661 gcggtagtct cgcacgagct gcgaacgcca ttgaatgcga tcttgggctg gacacgaatg
      721 ctgcgcagta gctccttctc cgaagagaag cggagccgcg ctctggagac catcgagcgc
      781 aacgcgaacg cccaagcgca gctgatcgag gacttgctgg acgtgagccg gatcatctcc
      841 ggcaagctgc gtctggacgt ggctagcgtc gacgtcgcca gcgtcctcga cgaggcgatc
      901 gacgtggttc ggcccgccgc gtatgccaag ggcatccggt tgaagcaaac gctcgacccc
      961 aatgccggcc cgattctggg tgacccgaac cgattgaagc aggtgatttg gaatctgctg
     1021 acgaacgcgg tcaaattcac gcccaagggc ggcagcgtgc atatcgcttt gcgcaagcgc
     1081 gactcgtcca tcgagatcgt cgtctccgac agcggccaag gcatcgaacc agagttcctc
     1141 gagcacgtct tcgagcgatt tcggcaggca gacgccacca cgactcgaaa gcacggcggc
     1201 ctcgggctcg ggctcgcaat cgtccgtagc ctggtcgagc tgcacggtgg caccgtccgt
     1261 gtgacgagcg agggattggg caagggcagt tcgttcatcg tgtgcctgcc catctctcca
     1321 cttcgggggg ctgaaatcga gagcccaccc gcagtgcgtc tcgtgatgaa gtcatcgctc
     1381 cagcccagta ccgagctcgc caacgtgcac gtgctcgtcg tggatgacga agacgacgca
     1441 cgagatctgg tcgccgagat gctaatcagc gcgggcgccg aggtctctac cgcggccagc
     1501 gcgcgcgagg cgctcgggtt cgtaagggag cgccggccgg acctggtcgt gtccgacatc
     1561 ggcatgcccg gtgaagacgg atattccttc atcaagcagc tgcgtgagct cccggccagt
     1621 caaggcgggc gtacaccggc cattgcgctg accgcctatg cacgcgtcga agaccgcacg
     1681 aaggctctgc tcgcaggttt caatatgcat gtgacgaagc cggtggagcc cagggagctc
     1741 atcgcattgc tgagtagctt ggccgctctg ttcgagcgga aatagcctga ccgtccgggc
     1801 cgggtaggca tgcaaagtta gtctcgactc ggattcccca gactttgcgc tcgcaaagca
     1861 agcgctcggc ccgttggctc gggcatgggc tggagcgaag cccgcaccgc agccgggact
     1921 ttcgcggtac aacggcagag tcgtgatgct cagcgaactg gctatcattc tggtgctcgt
     1981 gctggcgaac gggttctttt cgggtgcgga aatcgcggtg gttgccctcc gacgcgggcg
     2041 catcaaggag ctcgccgagc agggacgcgg cagcgctcga gcagtgctgg ccttgaggtc
     2101 acagccggag cgtttcctgg ccacggttca ggtaggtatc accgtggtcg gggcgacggc
     2161 ggcagccttc ggcggggcgg ccatcgcgga gcggctcgct ccgctgctcg cgcgggtgga
     2221 gtggttagcg caccacgccg aggggctagc gctcgggatc gtcgtgagtg tcgtttcttt
     2281 cctatcgatc gtggtgggcg agctcgtgcc gaagtcgctc gcattgcgca atgccgagcg
     2341 tttcgcgctg ctgatcggaa agcctctcct ctggttgtct tggctggcg
//
LOCUS       DASTKU010000868         3848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1510896, whole genome shotgun sequence.
ACCESSION   DASTKU010000868 DASTKU010000000
VERSION     DASTKU010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510896"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFQ61_30580"
     CDS             complement(<1..445)
                     /locus_tag="VFQ61_30580"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9958892.1"
                     /translation="MTVPSVPQPPQLGTWIRGWRLDRLIHVGNNACVYRAVAEEGSVV
                     GALKALRPSAIAHRTAAERFAREIAALQGLTHPAIPRLLDHGIDEDSLPWFVTEWLVG
                     WDLETHRVRLGGRLPPTEVVEIGVRLLDALAHAHAGGFVHRDVKPA"
     gene            complement(708..1604)
                     /locus_tag="VFQ61_30585"
     CDS             complement(708..1604)
                     /locus_tag="VFQ61_30585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9958893.1"
                     /translation="MLAAVVGSCQRAVEPAKAASVPQEGSPPHAERRDDLVQVRAGLR
                     LHLRCEGRGAPIVVFDSGLGLDGEYWHRGPAPLAAATSRFTRACTYDRAGRGRSDPAQ
                     VPHRQSTMARELHDLLANAGEPAPYVLVGHSMGGVIAQFLLAQHPASVAGMVLIDSSP
                     EPPPLTQIAPEMLAEWERNITRLEGLERESFMQGFEELRSSQRTLADKPLVVLVAGRA
                     LPTAGADSASRSQLEAQQRAQRQLVALSTNSVSVTAEYATHNLPYDAPTLVLESVRAV
                     VLAARRHTQLDAKALRQAAAPP"
     gene            complement(1814..2587)
                     /locus_tag="VFQ61_30590"
     CDS             complement(1814..2587)
                     /locus_tag="VFQ61_30590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958894.1"
                     /translation="MTGAWLNPAGGLRYHARALRYSRTLWDPFRWALGEWLLGFQPPE
                     RTLVIVGPSAGYNLQPFFLERFERVIGLEPDPIARYLFRRRIERAPLDRRPQLSFIAE
                     DQLLGEPERFLRLLETLGDCALLFSNIIGQMRVLVEADVESAPRLTRIREVIRSAVRD
                     RSFASFHDRVSGPIPPAFEPPMHGPSRLSDAEVIDLYDDSALSEQTPLLDHFTGGFFP
                     PDLPHSYFVWQLEPGIYHLIEAVSRTLGAGAATGAKPEP"
     gene            complement(2642..3391)
                     /locus_tag="VFQ61_30595"
     CDS             complement(2642..3391)
                     /locus_tag="VFQ61_30595"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HET9958895.1"
                     /translation="MKRESSAPYRVVPGSPPELIVYDEAQPCPYLEGRLARLPLRIPA
                     RPLTRSELELRLTTGDRRQGVFFYHTSCPNCTACEPIRIDVQRFVPGRTHRRTLRRGD
                     AALRVDLGPTLVDEARVNLYNRHKLLRGLGAGRAPIDLDGYREFLVLSSCDTFEIRYF
                     LADELVAVAIVDRSERALSAVYCFYDPAFEHLGLGTYSILKQIELCRAWRLRYLYLGL
                     YIEGSQHMAYKARFLPHERLLDGVWSEFTQP"
     gene            complement(3388..>3848)
                     /locus_tag="VFQ61_30600"
     CDS             complement(3388..>3848)
                     /locus_tag="VFQ61_30600"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET9958896.1"
                     /translation="APEIRGISEALGYSHGTGARPVTSEQRATLLEAARKVSRHAHAP
                     YSGYHVGAALLTRSGAVFTGCNVENASYGATLCAERAAIASMIAAGDREVVSVLVFTE
                     KGPPAMPCGICRQTLVEFATPDCSVYVATGTDQLETTLGALLPEPFVFRK"
BASE COUNT          760 a         1222 c         1247 g          619 t
ORIGIN      
        1 tcgcgggctt cacgtcgcga tgaacgaagc cccccgcgtg agcgtgcgcg agcgcgtcca
       61 ataaccgcac cccgatctcc acgacttcgg ttggcggcag ccttccgccg agacgcacgc
      121 gatgcgtctc caagtcccag ccgaccagcc actccgtaac gaaccacggt aggctatcct
      181 catcgatgcc gtggtcgagc aggcgcggga tggcggggtg ggtcagcccc tggagagcgg
      241 caatttcccg cgcaaaacgc tccgccgcgg tgcgatgggc aatcgctgaa ggccgcaaag
      301 ccttgagagc gcccacgacg gaaccttctt cggccaccgc acggtagacg caggcgttat
      361 tgccgacatg gatgagccgg tccaagcgcc agcctcgaat ccacgtcccg agctgcggcg
      421 gctgcggaac ggagggaacc gtcaccagcc tgtggatagc ggttcaccgg tcgaagctca
      481 agtcccggca ctcgtgggct gaggaaagcc gcacgaaagt ccgccatcgg cctactgccc
      541 gactcgaaga aagccccaaa caccgcgaat gacactcgcg cttgccccgg gcccgaccga
      601 cagaccgatg cattaccacc gcagacgcgc ctccgattcg ggcggcaggg ggcagcttgt
      661 ttgttgcgca acgatgagcc tactcacgag gcatatcaac agcgacttca aggcggggca
      721 gcagcctgtc tcagagcttt cgcatccagc tgcgtatgcc gacgagctgc aagcacgacg
      781 gcccgaactg actccagtac caaggtcggc gcgtcgtagg gcaagttgtg cgtggcatac
      841 tccgccgtga cgctcacgct attcgtggac aaggccacca gctgccgttg agcacgctgc
      901 tgggcctcga gctgactccg agaggcagag tcggcgcctg ccgtcggaag cgctcgcccc
      961 gccacgagca cgaccagtgg cttgtctgcc agtgttcttt ggctcgaacg aagctcctcg
     1021 aacccctgca taaaggactc gcgctcgagc ccttccagcc gcgtgatgtt gcgctcccat
     1081 tccgccagca tctccggcgc gatctgggtg agcggagggg gctccggtga tgaatcgatc
     1141 agaaccatgc ccgccacgga agccgggtgc tgcgccaata agaactgcgc aatgacgcca
     1201 cccatcgagt gccccacgag tacgtacgga gcgggctccc cggcgttggc tagcaagtcg
     1261 tgcagctccc gcgccatggt gctctgacga tgcggaactt gcgccggatc actccggccg
     1321 cgacccgcgc ggtcgtaagt gcaggctcgg gtgaaccggc tggtagctgc cgcgagcggg
     1381 gcggggcctc gatgccaata ttctccgtcg agccccaagc ccgaatcgaa aacgacgatc
     1441 ggcgctccac gaccttcgca gcgcaagtgg agtctgagac cagcgcgaac ttggaccaaa
     1501 tcgtcacgcc gctccgcgtg cggcggtgat ccttcctgag gaacacttgc cgcctttgcg
     1561 ggttccacgg cgcgctggca cgagcctact actgctgcga gcatcagtga atggcacaca
     1621 gcgcctagcc agggcatcga gcatagccag gatgtcgagt gcagccgaaa cacggcttag
     1681 agtaaaagac ggataggact cgtcaaatta acaagggcat gtccttgatc gcgtcgcgag
     1741 gttgtggacg tttgcagtgg gcgcaatgcg cggtttttgg agggccgcgg gtggaggtgt
     1801 ctgcgcgtga gagtcacggc tcgggcttcg cgcccgtagc cgcgccggcg cccaaagtcc
     1861 gactgacggc ttcaatcagg tggtagatac cgggctcgag ttgccagacg aagtagctgt
     1921 gcggcaggtc cggaggaaaa aagccgccgg tgaaatggtc gagcaagggg gtctgttcac
     1981 tcagcgccga gtcgtcgtag aggtcgatga cctccgcatc actgagccgc gaagggccgt
     2041 gcatgggagg ctcgaatgca ggcggaattg ggcccgagac gcgatcgtga aagctcgcga
     2101 acgagcggtc gcgcaccgcg ctgcggatga cctcgcggat gcgcgtcagg cgcggtgcgc
     2161 tttcgacgtc tgcctctacg agcacccgca tttgaccgat gatgttgctg aagagcaaag
     2221 cgcagtcgcc gagggtctcg agtaaacgca agaagcgctc cggctcaccg agcagctgat
     2281 cctccgcgat gaagctgagc tgcggccgac gatcgagcgg cgcccgctcg atccggcgtc
     2341 gaaacaggta gcgagcgatc gggtccggct cgagcccaat cacgcgctcg aatcgttcca
     2401 aaaagaacgg ttgcaagttg tagccggcgc tgggccccac gatgaccagc gtgcgctcgg
     2461 gcggttgaaa tccgagcagc cattcaccga gcgcccaccg gaacgggtcc caaagcgtgc
     2521 gcgagtagcg cagcgctcga gcatgataac gtaagccacc ggcggggttg agccaagctc
     2581 cagtcatcga ttcacccttt agctcagccg ccgcgatttg ccgcgcgggc caagagtacg
     2641 ctcagggttg agtgaactct gaccatacgc cgtcgagcaa acgttcgtga ggtaggaacc
     2701 gcgccttata ggccatatgc tgcgaaccct cgatatacaa cccaaggtat aggtagcgaa
     2761 gccgccaggc gcgacacagc tcgatttgtt tgaggatcga gtacgtgccg aggccgagat
     2821 gctcgaacgc gggatcgtaa aagcagtaaa ccgcagagag cgctcgctcg gagcggtcga
     2881 cgatcgcgac cgcaaccaat tcgtcggcca ggaaatatcg aatttcgaag gtgtcgcacg
     2941 aagataaaac caggaattcg cgatacccgt cgagatcgat cggcgcgcgt cccgctccca
     3001 gcccccgcag gagtttgtgg cggttgtaga ggttcacccg ggcctcgtcg acgagcgttg
     3061 gccctaagtc cacgcgcaag gcggcgtccc cacggcgcag cgtgcggcgg tgcgtgcgcc
     3121 cggggacgaa gcgctgcacg tcgatacgga tgggttcgca ggccgtacag ttagggcagc
     3181 tcgtatgata gaagaacacg ccttgacggc ggtccccggt ggtcaggcgc agctcgagtt
     3241 ccgagcgagt gagcgggcgg gcggggatgc gcagaggcaa gcgagcgagg cgaccctcga
     3301 ggtacgggca aggctgcgcc tcgtcgtaca cgatcagctc gggcgggctg cccggcacga
     3361 cgcggtatgg cgcgctcgac tcgcgcttca tttacgaaac acgaacggct ccggcaacag
     3421 cgcgccgagc gtcgtctcca gctgatcggt tccggtcgct acgtacacgc tgcagtccgg
     3481 cgtcgcgaac tcgaccaagg tttggcgaca gatcccgcat ggcatggccg gtggcccttt
     3541 ctcggtgaag accagcacgc tcaccacttc gcgatcaccc gcagcgatca tgctcgcgat
     3601 cgcggcgcgc tcggcgcaaa gcgtggctcc gtaactcgcg ttctcgacgt tgcagcccgt
     3661 gaaaacggcg ccgctccgcg tgagcagcgc cgcgccgacg tggtatcccg agtaaggagc
     3721 gtgggcgtgg cgactcacct ttcgagctgc ctccaaaagt gttgcacgct gctccgaagt
     3781 caccggccga gctccagtcc cgtgagagta gcccagcgcc tcagaaattc cacgaatctc
     3841 gggcgcac
//
LOCUS       DASTKU010000869         3540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_838939, whole genome shotgun sequence.
ACCESSION   DASTKU010000869 DASTKU010000000
VERSION     DASTKU010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838939"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..496
                     /locus_tag="VFQ61_30605"
     CDS             263..496
                     /locus_tag="VFQ61_30605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958897.1"
                     /translation="MTLDTETHKQITLYDLRATGITWRCLRKDYGPEIQEAAGHEQFD
                     TTNGYVRTARCSQAVSEIPFPRSRWASWWPIRL"
     gene            complement(521..595)
                     /locus_tag="VFQ61_30610"
     tRNA            complement(521..595)
                     /locus_tag="VFQ61_30610"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(560..562),aa:Glu,seq:ctc)
     gene            complement(619..689)
                     /locus_tag="VFQ61_30615"
     tRNA            complement(619..689)
                     /locus_tag="VFQ61_30615"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(655..657),aa:Gln,seq:ctg)
     gene            complement(935..1912)
                     /locus_tag="VFQ61_30620"
     CDS             complement(935..1912)
                     /locus_tag="VFQ61_30620"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HET9958898.1"
                     /translation="MKPEGLLCAFSLAPVAEIPNPSEKDPSELCPRDEAPLWLHFSLL
                     DNRSRRYLRDHVELPEEAREALLGPDARARAQAFSNGFVVFLGDFHRDFSSDPEAFGA
                     LRVYVSPYLMLTCRKHPLKSVERIHAKIHHGDIEADSPLELFDRLLACLVEGFVSLAS
                     DLADSLDDAEDRILAGRIEDHASQLGQMRRLLARLRRYAGANRTALAHLPEHIAGWTT
                     PEERLRLREIIDRFDSVGQDLDLVTERARLLQEEIAARLNEATNRNLYVLSIVTVAFA
                     PITLITGVFGMNVGGLPFSVSHGGFWVVVCLMVITVLSTLAMLRRAGVL"
     gene            complement(2173..2865)
                     /locus_tag="VFQ61_30625"
     CDS             complement(2173..2865)
                     /locus_tag="VFQ61_30625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958899.1"
                     /translation="MGHAILIVLAAAYTVQLGVVVGLWALAKFGGRPMVRVQLGYPAL
                     LRFSAWGTSVEVGLPIGWNAWFAEQRGVALASAAPIWRDAETEKLQRERAEKENRLAQ
                     STQHARWTPLLYLALWSLGLALLGFDVALAARHSVNHVQQIFSELWAPWSRGADAVRR
                     ATNFLANAPLLQGYGIILVRHGAVRILTAVLRIILLSVGWEGAVLITALAELAICALW
                     IVAVLAMLLRSI"
     gene            complement(2877..>3540)
                     /locus_tag="VFQ61_30630"
     CDS             complement(2877..>3540)
                     /locus_tag="VFQ61_30630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958900.1"
                     /translation="TVAAWDHPEEFFSAFDWLEVDARSDGCRSFLQACQGRPLLAKVA
                     RSRATPDVFTELIRLGRAGPSVRRRLLTSLARSFFMNVQDWCGLCKRLSRARRPLASH
                     LLETLALSGITEAVSAPATEWLEESEYFGARVSKLLQYLLRGAGALATYSIADWAFKS
                     GGPDSHISGSQVWEVLLIVVALLLLQSWWDARRRVDPQSRELFSKLWAFAEASRSAGG
                     HR"
BASE COUNT          698 a         1092 c         1163 g          587 t
ORIGIN      
        1 acctgcgccc agtgctcggg acaagcaccc acgcgcgtgg acacgagagg attgcgaacg
       61 cggggccccg agcctcgacg caaagatcac cgggaaggag tgctcgtgga agaccgcggc
      121 gaatgcgtgg tcgctgttca ctcaagctca tgcaccaaga gtcgggcggg caggggctcg
      181 tctacgcacc gatgccgccg atggaggatt gggccgataa gttccgaagg cacctcgagc
      241 gggcaggggt gaggcgcgcg cgttgaccct cgacaccgaa acgcacaagc agatcacgct
      301 ctacgattta cgagcgacgg gcatcacgtg gcgctgcctt cggaaggatt acgggcccga
      361 gatccaggag gccgcgggtc acgagcagtt cgacaccacg aacgggtacg tccgcaccgc
      421 ccggtgttca caggccgtgt cggagatccc tttcccgcgc tcccgatggg ccagttggtg
      481 gcccattcgg ctgtaagtca cgggaatcgt ggagctatgt tggcgacccc agcgggaatt
      541 gaacccgcgt taccggcgtg agaggccgat gtcctaaccg ctagacgatg gggcctaagt
      601 atcgaagaaa gtgctgagct gggggactag gattcgaacc tagatagcaa gagccagaat
      661 cttgcgtcct gccgttagac gatcccccaa gggcttggag gctagcgcga gagcaaaggt
      721 cgctccacgc gcccggccgg gaaatccgag gagggttgcc cgttcggggc cgcgtaacta
      781 tcgccgttcc tgggtttgcg caaggggttc gccaccgatt tctacgaccg aacggacgaa
      841 ggcgccggcc cataggccag acggcgactg catcgacctc ttggggcttc acctggtcgg
      901 cgggaagtcg cgcggtcgcg gccaccgggc accttcaaag cacgcccgcg cggcgcagca
      961 tggcgagggt gctgagcacg gtgatgacca tgaggcagac gacgacccaa aagccgccgt
     1021 gggatacgct gaagggcagc ccaccaacgt tcatgccgaa gacccccgtg atgagggtga
     1081 tcggcgcgaa agccaccgtg acgatggaga ggacgtagag gtttcggttg gtcgcttcgt
     1141 tcaggcgcgc cgcgatctct tcttggagca gccgggctcg ttcggtgacg agatcgaggt
     1201 cctgcccgac cgagtcgaag cggtcgatga tttcgcgcag acgcaggcgc tcttctgggg
     1261 tggtccagcc cgcgatgtgc tcgggtaagt gagcgagcgc ggtgcggttc gcgccggcgt
     1321 agcgccgtaa gcgcgcgagc aggcggcgca tctgtccgag ctgacttgcg tgatcttcga
     1381 tgcgcccggc aaggatgcga tcttcggcgt cgtcgagcga gtccgccaag tcactggcaa
     1441 ggctgacgaa tccttcgacc aggcatgcca gtaggcgatc gaagagctcg agtggtgagt
     1501 cggcctcgat gtcgccatgg tggatcttgg cgtggatgcg ttcgacgctc ttgagcgggt
     1561 gtttgcggca cgtgagcatg agataggggc tcacgtagac tcgaagtgcg ccgaacgcct
     1621 cgggatccga gctgaaatca cggtgaaagt cgcccagaaa gacgacgaag ccgttcgaaa
     1681 aggcttgggc gcgagcgcgc gcgtcgggcc caagtagggc ttcgcgcgct tcctcgggca
     1741 gctcgacgtg gtcgcgcaag taacgacggc tgcggttgtc gagcaggctg aagtgcaacc
     1801 agagcggggc ctcgtcgcgg ggacagagct cgctcggatc cttttcgctg gggttcggga
     1861 tctccgcgac gggcgcgagg ctgaacgcgc aaagaaggcc ttctggtttc actcgacctt
     1921 tttcgacctg ctatcggctt ggccgtggtc gtctcttacc aagcgatgcg cgagaaccaa
     1981 cgcgatggcg ttgccctcga caacacgtca cgtcggcgcc actcgttcgt catcgaacgc
     2041 gagcttcgcc ggttcgctgg ctgctcttca tgtgcgcgta tttcgcgcgg gcctgctcac
     2101 gactcagcag aggagctcag caggagctca gcgtgctgcg cgtcggggaa ggcgaggggc
     2161 ggcagcgcag catcatatcg agcggagtaa catcgcaagc accgcaacga tccagagcgc
     2221 gcagatagcc agctcggcga gagccgtgat gagcactgcg ccctcccagc cgacgctcaa
     2281 gaggatgatc cgaaggactg cggtcaggat acgcacggct ccgtgccgga ccaggataat
     2341 gccgtaccct tgaagtagcg gggcgttcgc gagaaagttg gtcgcgcgcc gcacggcgtc
     2401 ggcaccgcgc gaccaaggtg cccagagctc gctgaatatt tgctgaacat ggttcactga
     2461 atgtcgagcc gcgagcgcca cgtcgaatcc gagcagggcc agaccgaggc tccacaacgc
     2521 gagatacaat agtggtgtcc accgcgcgtg ctgcgtgctt tgcgcgagcc tgttttcctt
     2581 ctcggcgcgc tctcgctgca gcttttccgt ttcggcgtcg cgccagatgg gggcggccga
     2641 agccagcgcc actccgcgtt gttctgcaaa ccaagcattc cagccaatgg gcagcccgac
     2701 ttcgacactc gttccccaag ccgaaaaccg cagcaacgcg ggatacccga gctggacacg
     2761 caccatcggc cgtccgccga atttggcgag cgcccacagc cctacgacga ccccgagctg
     2821 aaccgtgtac gccgctgcaa gcacaatcag gattgcgtgt cccatattcc ctcgcgtcat
     2881 ctgtggccgc ctgcactgcg cgaagcctct gcaaacgccc acagcttcga aaagagctcc
     2941 cggctttgtg gatcgacgcg tcggcgcgcg tcccaccatg actggagcaa gagcagcgcg
     3001 accacaatca gcaacacttc ccagacctgg gacccggaaa tgtgtgaatc tggtccgccg
     3061 gatttgaaag cccagtcggc aatcgaatac gttgcgagcg cacctgcgcc gcggagaagg
     3121 tattggagta gcttgctcac gcgcgcgccg aaatattcac tctcctcgag ccactcggtc
     3181 gccggcgcac tcacagcctc ggtgatacct gaaagtgcga gtgtctcgag caagtgcgat
     3241 gcaagcggtc gccgagctcg cgataggcgt ttgcacaaac cgcaccagtc ttggacgttc
     3301 atgaagaacg agcgagcgag tgacgtcaac agccggcgtc gaacgctcgg gcccgcgcgc
     3361 cccagtcgaa tgagctccgt gaaaacgtcc ggcgtcgctc gcgaacgcgc gaccttcgcg
     3421 agaagcggtc gaccctggca agcctgcaga aaggagcgac aaccgtcgct gcgcgcgtcc
     3481 acctcgagcc aatcaaaagc cgagaagaac tcctcgggat gatcccaggc cgccaccgtg
//
LOCUS       DASTKU010000870         8650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1732947, whole genome shotgun sequence.
ACCESSION   DASTKU010000870 DASTKU010000000
VERSION     DASTKU010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8650
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732947"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2110)
                     /locus_tag="VFQ61_30635"
     CDS             complement(<1..2110)
                     /locus_tag="VFQ61_30635"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET9958901.1"
                     /translation="MKQIDARKLVQWVLRHARWLSVIVLLVTIVAGYRTVRTYGALRS
                     DLEELLPVQAPSVAALTALRDRLPGVRHLGVVVHVEKPQAQAAAFAFLDRLAERVRTY
                     PKDMVASVRVNSQEERTFAETYALQLMEPADVRSLRESVERLRDWQVSRGMGLDLEDG
                     TDEAKPVVPFAELRAKYEARYGVPAKTPDGRFLSADGKNVVLLIRTAGHTTSQRDDSR
                     LLDRVRADVRALGFPNAFDASLELGYAGDVASRVEEAHGLESDLTLSGIVVMLLELLV
                     VAFFYRSLRSLPILFVPLVTGTFAAFAIAALPPLSIRYLNSNTAFLGSIVIGNGINSG
                     VILLARYEEERRLGHSVAEAIEIAVAGTWRATLAAALAAACAYGSLVFTEFRGFNQFG
                     WIGGIGLITCWLSAYSVLPLMLKWLGAGVGVSPRRQAQAQPSSGSPGWLGRLTLGHPR
                     AVLLVAGALAVASVAGLVHRSSDIVEYDLSKLRRRDSWENGERFWGKRMDSAFNRYLT
                     PTVLLADSRQEARELEARLRDLMQRRAAGDLIGSVRSADMLLPETRAESAREASKLKA
                     ILTPRLLKEISSSDQELVQRALSERSLVPLEAHDVPDSLAMGFRERSGRMDRNVIVFP
                     KLTTGTWDAARLDGYANDLRRAATFDGHARPVAGSLLLSSDIARAMKADGPKATLLSL
                     LSVIAICWIAFRDEKRSRGAS"
     gene            complement(2148..3206)
                     /locus_tag="VFQ61_30640"
     CDS             complement(2148..3206)
                     /locus_tag="VFQ61_30640"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET9958902.1"
                     /translation="MSKTALPGAHSDRGSASSDGNLFPLNSNLSLKTLPRRLCQRMWS
                     LWGPALVLPFIPALYALVPAWRGDLRPEHVALGLVVPIVAFWGPRAKQFLCDVSPYIA
                     VAVGYDLVRYVRPLFVTEHRVLGQSLRNAELALFGVGPNTTLQDYFNAHNAPAFDLLF
                     AVPYAIFVYVAALYAIYLYFVDRPRMRHYLLSFAIANYLAFACWLIFPAAPPWYLRRY
                     GAHIDIAALPSPAALVRVDALLGVHYFRDFYARASSVFGALPSMHCAYPAIGLLTAYR
                     AGTWRTRSIHLIYSLTMVVAAVYLDHHWVIDCIAGLVMATVSVRLAGYWLKRREAAWT
                     SAAVPLPAASSALQTQKL"
     gene            complement(3252..4043)
                     /locus_tag="VFQ61_30645"
     CDS             complement(3252..4043)
                     /locus_tag="VFQ61_30645"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET9958903.1"
                     /translation="MNLLEHDIPNSGTGWIPSARSAPVASPRANLHAIRSTAPAAARL
                     AGTIWERSDVAVLAYRSFYALGVGLGKLGISANAITYTSVVLAGLSAVAAAQGHFLVS
                     ALVLALGGLCDALDGIVARSTGRVSKYGALLDSTVDRVSDVLPLIGMMMFQAKQPWFV
                     LPPAFALVTSFVIPYVRARAEALGVVLPGLFMRRPERTLMLIACLAVAGATPQPGLAL
                     LVGSSIMALLNAYGCFLVLRSAQRALGQAEIPAPAAASDNPTLSA"
     gene            4443..4931
                     /locus_tag="VFQ61_30650"
     CDS             4443..4931
                     /locus_tag="VFQ61_30650"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HET9958904.1"
                     /translation="MPAHFVRTVLPGDEAAKWIATWFGCGRSPIAPGTVGTLGTLPLY
                     WAMRSLGPLPYAAITLALTAIGTWAAERTAQALGDEDPSAVVIDEVAGTLIALGFARA
                     GGLPAELLSVVLFRFFDIKKPGPIDSVQHLKPAGAGIMADDLLAGFAAGLSVRALRWL
                     LP"
     gene            complement(5002..6207)
                     /locus_tag="VFQ61_30655"
     CDS             complement(5002..6207)
                     /locus_tag="VFQ61_30655"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9958905.1"
                     /translation="MAAPSVSRSLLVAIAVCACSALAPAAPSNDAHREPPRRDGQAPK
                     APAPSVVSQTTKSKPGVPTAPAAGTHPSGSAKAPDKPTPERASAEKSSPAKRPEPAKA
                     AEPVACPAGMLLVDGDYCSDVDYECLKSWYDPSNKKTICEEFKPESKCVGKKTKKRFC
                     IDQYTWPNEKGARPEVMNRFHQAELKCAAVGKRLCTESEWTLACEGPRMLPFPYGYVR
                     DTSKCLGDVEWDAPDMKKVAARDPEELARLWKGVRNGSQPGCVSEYGVADLPGNVDEV
                     VANETQDGSWRAKYDSVHTGGPWYKGTRNQCRPKIYTHDEGFYYYFLGFRCCAEPDGK
                     ETDFRTPRQIKEGWKLERVERIAGFSLAELKTKLEEKKQGNCRCSERDVRCKTMCGTL
                     LGPAAKDYR"
     gene            complement(6244..7077)
                     /locus_tag="VFQ61_30660"
     CDS             complement(6244..7077)
                     /locus_tag="VFQ61_30660"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET9958906.1"
                     /translation="MSVILTFFIGHWLSSVFCQTFFLHRYGAHSQFELSKGWERFFHL
                     LTYLTQGSSYLNPRGYAILHRMHHAYSDTQKDPHSPLFHSNAFSMMWATKKRYDAFAY
                     YREKPEARFDGGVPEWRALDRLGQSWPMRMVWVLGYTSVYVAFAPSLWWFLLLPAHFV
                     MGPIHGAIVNWGGHKYGYRNFKSDDASRNTLVFDFVTLGELFQNNHHEFAMSPNFAAR
                     KFEVDPTYPVILLLEKLGIVRNLSGQRARLSPVRSGAVAGKLPEIPGAGVEAADEIPA
                     E"
     gene            7267..7950
                     /gene="pyrE"
                     /locus_tag="VFQ61_30665"
     CDS             7267..7950
                     /gene="pyrE"
                     /locus_tag="VFQ61_30665"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET9958907.1"
                     /translation="MLAPYKAEFIEFMVRAGVLSFGDFVTKSGRKTPFFVNTGRYGTG
                     SDLLRLGRFYAQAIVDRLGEEFDVLFGPAYKGIPLVAATSIALFEQHQQNAGFVFNRK
                     EAKDHGEGGLLIGQPLRDGSRVLIVEDVTTAGTSVRECVPLLRAAARVQIAGLVVSVD
                     RMERGTGPLNALAELQLELGAPCFSIVTIEEVMSFLRGRQIDDREIVTDSIYERFLKY
                     RSEYGGKTA"
     gene            8037..8549
                     /gene="purE"
                     /locus_tag="VFQ61_30670"
     CDS             8037..8549
                     /gene="purE"
                     /locus_tag="VFQ61_30670"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET9958908.1"
                     /translation="MATPSPSKPLVGVIMGSQSDWETMRHAAQTLEELGVPHEVRVVS
                     AHRTPDLLFEYANSAESRGLAAIICGAGGAAHLPGMVAAKTVLPVLGVPVQSHALNGL
                     DSLLSIVQMPAGIPVGTLAIGKAGATNAALFAAALLGTSIPEYREALRRYREQRTQSV
                     LDSPDPRQSS"
BASE COUNT         1710 a         2797 c         2608 g         1535 t
ORIGIN      
        1 ggctcgcccc ccgagaacgc ttttcatccc tgaacgcgat ccagcaaatc gcgattacgg
       61 atagtagcga gagcagcgtg gccttgggac catccgcctt catggcccgc gcaatgtcgc
      121 tcgagagcaa cagcgacccc gccaccgggc gcgcgtgtcc atcgaacgtc gccgcgcgac
      181 gcaggtcatt cgcatagccg tcgagccgcg ccgcatccca agttcccgtc gtgagcttgg
      241 ggaacacgat tacgttgcgg tccatgcgac cgctgcgctc ccgaaagccc atggccagag
      301 agtcagggac atcgtgcgcc tcgagcggca ccagcgaacg ctccgagaga gcgcgctgca
      361 cgagctcctg atccgatgaa gatatctcct tgagcagacg cggggtgagg attgctttga
      421 gcttgctcgc ttcccgcgcc gattcggcgc gagtctccgg cagtagcatg tctgcgctcc
      481 gcaccgagcc aatcaagtcg cccgcggctc gacgttgcat caagtcacgc aagcgcgcct
      541 ccagttccct cgcctcttgg cgcgaatccg ccaacagcac ggtgggggtc agatagcgat
      601 tgaacgccga atccatgcgc ttgccccaaa aacgctcgcc attctcccaa gagtctcggc
      661 gtctgagctt ggaaagatcg tactctacga tatccgacga gcgatggact agacccgcta
      721 ccgaagcgac tgccaacgcg cccgctacga gcagcaccgc acgaggatga cccaacgtca
      781 gccttccgag ccagcctggg gatccactag acggctgcgc ctgagcctga cgccgcggcg
      841 atacgcccac gcctgcgccc agccacttca gcatcaatgg caacacgctg tacgccgaca
      901 gccagcacgt gattagtccg attccgccga tccagccgaa ctggttgaag cctcgaaact
      961 cggtaaagac cagcgagcca taggcgcacg cggcggcaag cgcagccgcc aaagtggccc
     1021 gccacgttcc ggctaccgcg atctcgatcg cctcggccac ggaatggccc aaccgacgct
     1081 cctcctcgta gcgcgccagt aagatgacgc cgctgttgat accgttaccg attacgattg
     1141 aaccaaggaa tgcagtattc gagttcagat atcgaatcga tagcgggggc agcgccgcga
     1201 tcgcgaacgc ggcgaaggtc cccgtcacca ggggtacgaa caagatcggc agcgagcgca
     1261 gcgaccgata gaagaacgcg accacgagca gctcgagcag catcaccacg atgcctgata
     1321 acgtcagatc ggactccagc ccgtgcgctt cctccacccg tgacgccacg tccccggcat
     1381 aaccgagctc caagctcgcg tcgaacgcgt taggaaagcc cagcgcgcga acgtcggctc
     1441 gcacgcgatc gagcaatcgc gagtcgtcac gttgagacgt cgtgtgaccg gcggtgcgga
     1501 tcaacaacac gacgttttta ccgtctgcag acaagaaacg accatcggga gtcttggccg
     1561 gcacgccgta gcgcgcctcg tacttcgcac gcagctcggc gaacggcacc acgggcttcg
     1621 cctcgtccgt gccgtcctcc aagtccagcc ccataccgcg agagacttgc cagtcccgca
     1681 aacgttccac cgactcgcgc agacttcgga cgtccgccgg ctccatcaac tggagcgcgt
     1741 aggtctcggc gaaggtacgc tcttcctgcg agttcacccg caccgaagcc accatgtcct
     1801 tcggataggt gcgaacccgc tccgcaagtc gatccaagaa cgcgaaggct gcggcctgag
     1861 cctgcggttt ctctacgtgc acgaccacgc cgaggtgccg cacaccaggc aaccgatcgc
     1921 gcaatgccgt cagtgccgcc acgctaggag cctgcacggg caacagctcc tccaagtcac
     1981 tacgcagagc gccgtatgtc cggaccgtgc ggtacccagc cacgatcgtt accagcagca
     2041 cgatgacgct cagccaacgc gcatggcgaa gcacccactg cacgagctta cgcgcatcga
     2101 tctgtttcat taatcgcctg atttcgtcgc ggttctaggt cgctacttca aagcttctga
     2161 gtctgtaatg ccgaggacgc ggccggcaac gggacggcgg cgctcgtcca agccgcctcg
     2221 cgccgtttga gccaataacc agcgagacgc accgaaactg tcgccatcac cagcccggcg
     2281 atgcagtcga ttacccaatg atggtccaga tagaccgccg ccaccaccat ggtcagggag
     2341 taaatgaggt ggatgctacg cgtccgccac gtcccggctc gataagccgt gagcaacccg
     2401 atcgcgggat aagcgcagtg catcgagggt aacgcaccga acaccgaaga ggcacgcgcg
     2461 tagaagtccc ggaaatagtg cactcccagc agcgcatcga cgcgcaccaa ggccgccgga
     2521 ctcggcagag ccgcaatgtc gatgtgagca ccgtaacgtc ttaggtacca agggggcgcc
     2581 gcgggaaaga tcagccagca ggcgaacgca aggtagttcg cgatcgcgaa cgaaagcaga
     2641 tagtgcctca ttcgcggccg atccacgaag tacaggtaga tggcgtacaa ggccgccacg
     2701 taaacgaaga ttgcgtacgg caccgcaaac agcagatcga aagccggcgc gttgtgagcg
     2761 ttgaaatagt cctgaagggt ggtgtttggc cccaccccga atagcgccag ttcagcgttc
     2821 ctgagcgatt gccccaacac gcgatgttcg gtgacgaaca gcggccgcac gtaacgaacc
     2881 aggtcgtagc cgaccgccac ggcaatatag ggtgagacgt cgcacaagaa ctgctttgct
     2941 cgcgggcccc aaaacgcaac gattggaacc accagcccca gtgcgacgtg ctccggacgc
     3001 aagtcgccgc gccatgccgg caccagcgca tacaacgctg gaatgaacgg caacaccagg
     3061 gcgggtcccc acaaactcca catccgctgg cataaccgtc gaggcagcgt cttgaggctc
     3121 aggttcgagt tcagcgggaa tagattgcca tcggaggacg cggaccctcg atccgagtgc
     3181 gcgccgggca gagcagtctt cgacatagcc agctcgaaac ctgtgagctt ctcgagaaca
     3241 gccgccccgc ctcaggcgct caacgtaggg ttatccgacg cggcagcagg agcgggaatc
     3301 tcggcctgtc cgagcgccct ttgagccgac ctcaacacca aaaaacaccc gtaagcattg
     3361 aggagcgcca tgatggaact tcccaccagc aatgccaaac ccggttgcgg tgtcgccccc
     3421 gcgaccgcca agcaggcgat caacatgagc gttcgctccg ggcgccgcat gaacagcccc
     3481 ggcaaaacta cgccgagagc ctccgcgcgc gcgcggacgt acgggatcac gaaagaggtc
     3541 accagggcaa aggcgggggg cagcacgaac caaggctgct tggcctggaa catcatcatg
     3601 ccgatcagcg gcaacacgtc cgaaactctg tcgacggtgg agtccaacaa cgcgccgtac
     3661 ttgctgactc ggcccgtact gcgggcgacg atgccgtcga gcgcgtcaca taacccaccg
     3721 agggcgagga cgagagccga cacgaggaag tgaccttgtg ccgcagccac ggcggacagt
     3781 cctgcgagca cgacggacgt ataggtgatc gcgttcgccg aaataccgag cttccccaag
     3841 cccacgccta gtgcgtaaaa agaccggtag gcgagcaccg ccacgtcact tcgttcccag
     3901 atcgtgccag ccaagcgtgc agcagccggc gccgtggatc ggatggcgtg aaggttggcc
     3961 cgcggcgacg ctaccggagc cgaacgagca gaaggaatcc agccggtacc cgaattcgga
     4021 atgtcgtgtt cgaggagatt caccgaggtt cctacattgg tcacaacgcg acgcatcgca
     4081 agcgccatgc cgacgactcg agaacggagt cttgcggttt tcattcctac gtctctcgct
     4141 cccaacagtc gtttccgcgt tcacgtccct gctcgtgcat gccttcgaat gtccgctcta
     4201 cagcggcgtt agaaccgatc gtcaaccatc gtagacaggt gttcgcggac acgtgtccgc
     4261 gttgccacac tgagtctgta ttgatgcata acacgcgaca cacctgttgt gtcctgcagt
     4321 cgttcacgcg cggatcatct tgagatgccc attcacggag cgtgagatgc tcgtaccagc
     4381 gcgcttcggc tcaccgagtt cgagttccat ttcgagcaga tgcccgatat ggtgctcgcg
     4441 tcatgcctgc acatttcgtg cgtaccgttt tgcccggtga tgaggccgcg aagtggatcg
     4501 ccacttggtt cggctgcggg cgttcaccca tcgcgccagg cacggtggga acgctcggaa
     4561 cgctgccact gtactgggca atgcgttcgc tcggtcctct tccttatgct gcgatcacgc
     4621 tcgcactgac ggctatcggt acctgggccg cggagcgaac agcgcaagcc cttggcgatg
     4681 aagatccgag tgcggtggtt atcgatgagg tggcgggcac cctcattgcg ctgggcttcg
     4741 cgcgcgcggg cggtttacct gccgagctgc tgtcagtcgt gttgttccgc ttcttcgata
     4801 tcaagaagcc gggaccgatt gatagcgtac agcacctcaa gccggcgggg gccggcatca
     4861 tggccgacga cctgctcgcg ggtttcgccg cgggtctatc cgtccgagcg ctgcgctggc
     4921 tgctgccgtg aggaacgcgc gcccttcatg aagcgtccgg cgagggccac ggccactcgc
     4981 ctcgcggatt tcgatcgaga gctagcgata gtctttagcg gccggcccaa gcagcgttcc
     5041 gcacatcgtc ttgcagcgaa cgtcgcgctc gctgcagcga cagttccctt gcttcttttc
     5101 ctcgagcttg gtcttcagct cagcgagcga aaaacccgcg atgcgttcga cgcgctcgag
     5161 cttccagcct tccttgatct ggcgcggggt tctaaagtcg gtctcctttc cgtctggctc
     5221 tgcgcagcat cggaaaccaa ggaagtagta atagaagccc tcgtcgtggg tgtagatctt
     5281 ggggcggcac tggttccggg tgcctttgta ccaaggtcct ccggtgtgca cgctgtcgta
     5341 tttcgcacgc cagcttccgt cctgcgtttc attcgcgacc acctcgtcca cgttgccggg
     5401 caggtccgcc actccatatt cgctgacgca accgggctgg ctgccgttac gaacgccctt
     5461 ccacaaccgg gcaagttctt cggggtctcg cgccgccacc ttcttcatgt ccggggcatc
     5521 ccactcgacg tctcccaagc acttgctggt gtcgcggacg taaccgtacg gaaatggcag
     5581 catgcgtggc ccctcgcacg ctaacgtcca ctccgactca gtgcagagcc gcttccccac
     5641 cgccgcgcat ttcagctcgg cctgatgaaa gcgattcatc acttccgggc gcgcgccctt
     5701 ctcgtttggc caggtgtatt ggtcgatgca gaagcgcttc ttggtcttct tgcccacgca
     5761 cttggattcg ggcttgaact cctcacaaat cgtcttcttg ttggaggggt cgtaccagct
     5821 cttcagacac tcgtagtcga catcgctgca gtagtccccg tccaccagaa gcattcccgc
     5881 cggacaggcg acgggctcgg cggccttggc tggttcggga cgcttggcgg gcgaggactt
     5941 ctcggccgat gctcgctcgg gcgtgggctt gtcgggggcc ttggctgaac ccgaagggtg
     6001 ggtgcctgcg gcgggcgcag tcggaacccc tggcttggat ttagtcgtct gagacacgac
     6061 cgacggcgcc ggagccttcg gagcctggcc atctctacga ggtggttcgc gatgcgcgtc
     6121 gttgctcggc gccgcgggag ctagcgccga gcaggcacat acggcgattg cgacgagcag
     6181 agaccgagaa acggaagggg ccgccatgct gggcggcgca taccacggag cgggcggttc
     6241 gggctattcc gcggggattt cgtcggcagc ttcgacgcca gcgcccggta tctccggaag
     6301 cttgccggcg accgcgccgg aacgtaccgg cgagagccgc gcacgctgac cgctcagatt
     6361 tcgcacgatg ccgagcttct cgagcagcag gatcaccggg taagtaggat ccacctcgaa
     6421 cttccgcgcg gcgaagttcg ggctcatcgc gaattcgtga tgattgtttt gaaacaactc
     6481 cccgagcgtc acgaagtcga acaccagcgt gttacgcgaa gcatcatcgc tcttgaagtt
     6541 tcggtacccg tacttgtgac cgccccaatt cacgatcgcg ccgtggattg gacccatcac
     6601 gaagtgagcg ggcaacaaca ggaaccacca caagctcgga gcaaacgcca cgtaaaccga
     6661 ggtgtagccc agcacccaaa ccatgcgcat cggccaggac tgcccgaggc gatcgagcgc
     6721 acgccactcc ggcaccccac catcgaaacg ggcctccggc ttttctcgat aataggcgaa
     6781 cgcgtcgtag cgcttcttgg tcgcccacat catcgagaaa gcattcgaat ggaacagcgg
     6841 cgagtgcgga tctttctgcg tgtcgctgta cgcgtggtgc atccgatgaa gaatcgcgta
     6901 cccgcggggg ttcaggtagc tcgacccctg agtcaagtac gtcagaaggt ggaaaaagcg
     6961 ttcccagccc ttcgatagct cgaactggct atgcgctccg taccgatgga ggaagaacgt
     7021 ctgacaaaac acagaggaga gccaatggcc gatgaagaag gtcaggatca cgctcatcgc
     7081 ggggcaaact agaacgtgcg ccaaaatttc tgggtcatgc tttcgtcatt tccgacgttt
     7141 taggcgggca cttgtgcgct ttcgggagcg cgactctggc ggggttcccc ggaaaacgga
     7201 cccgaaaccc gccccccgac agatcggcac gcgggaccgg cggcctttcg tgctaagggg
     7261 acgcccgtgt tagcgccgta caaagctgag ttcatcgagt tcatggtgcg cgccggagtg
     7321 ctctccttcg gggacttcgt cacgaagagc ggccgcaaaa cgcccttctt cgtgaacacg
     7381 ggccgctacg ggaccgggtc tgacctgctg cgcttgggtc gtttctacgc gcaggccatc
     7441 gtcgatcgcc taggtgagga gttcgacgta ttgtttggtc cggcgtacaa aggaatcccc
     7501 ttggtggccg cgacctctat cgcgctgttc gagcagcacc agcagaatgc gggcttcgtg
     7561 ttcaaccgga aagaagccaa agaccacggc gagggtgggc ttttgatcgg gcagccgctg
     7621 cgcgacggct cgcgggtgtt gatcgtcgag gatgtcacca ctgccgggac ctcggtccgc
     7681 gaatgcgtcc cgctgcttcg ggccgcggcg cgggtccaga tcgcggggtt ggtcgtatcc
     7741 gtagaccgaa tggagcgcgg cactgggccg ctcaatgctc tagccgagct gcaactcgaa
     7801 ctgggcgcgc cttgcttttc gatcgtgacg atcgaggaag tgatgagctt cctgcgcggc
     7861 cgccagatcg atgaccggga gatcgtgacc gactcgatct acgagcgatt cctgaaatat
     7921 agatcggaat acggcggaaa aaccgcgtaa attcgcggtt tcggcgcgcg cccggtgcca
     7981 gcacggatcg aatccgcttg ctcggcaagc atcgggcgtt aaagtccgcg cctctcgtgg
     8041 ctacgccttc cccttccaaa cccctcgtgg gtgtcatcat gggttcccaa agtgattggg
     8101 agaccatgcg tcacgcggct caaaccctgg aggagctcgg tgtcccgcac gaagttcggg
     8161 tcgtctccgc gcatcgcacg ccggacttgc tgttcgagta cgcgaacagc gccgaatccc
     8221 gcgggctcgc cgcgatcatc tgcggagctg gcggcgcggc ccatctgccc ggcatggtcg
     8281 cggccaagac ggtgttgccc gtgctgggcg ttccggttca aagccatgcg ctgaatgggc
     8341 tcgactcact cctctccatc gtgcaaatgc ccgccggcat tccggtcggc acgttggcga
     8401 tcggcaaggc gggagcaacg aacgctgcct tgttcgctgc cgcgctgctg ggtacctcta
     8461 tccccgaata tcgtgaggcg ctgcgtcgct accgcgagca gcgtacccaa agtgtcctcg
     8521 attcccccga tccccgacag tccagctgaa gccgggaact cggctagcgc ggcgaacgcg
     8581 gccgattcag aaatccccgg cgcatcggag gtcctgattg caccccgggt cggcgtgctc
     8641 ggagcgggcc
//
LOCUS       DASTKU010000871         8474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_615958, whole genome shotgun sequence.
ACCESSION   DASTKU010000871 DASTKU010000000
VERSION     DASTKU010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8474
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615958"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VFQ61_30675"
     CDS             <1..987
                     /locus_tag="VFQ61_30675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958909.1"
                     /translation="LLSPTIRRFDILVLNFERINLFLENFHKLRNFDPERDRIVISDC
                     SNNAGRERERVARFANERGWILGGPQITLSRRPNWGLAEGARIDYFTQLLRDDTVAPY
                     VWQFQEHYLDTTSDYSRWPASDRARAGQVKADILPDGMVIDLDICEQAFSNPDVAVLC
                     APRCGLGIFSHPDGREWLYADGANFGFRTSVARRVLSNQLLDDYRLTFDASYRWAIFM
                     EFEYSRLFSEGAWFDLVHEMAFRDVAEVRAAEQQLGKPISTIAEESSARRHQRYERRA
                     RLVNKFPELLRQGLLATHFKALGIAQTKLIGPSRAKLLAMNIDSPQWFRDVW"
     gene            977..1972
                     /locus_tag="VFQ61_30680"
     CDS             977..1972
                     /locus_tag="VFQ61_30680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958910.1"
                     /translation="MSGERPRKIVRNLIGAAIIACVAYFFLRALRDNWDRLRALEFEA
                     HYAALALTALSIAGSNLLGTAAWQAAMNTFAKRPLSFSESIATVNISSLTKYVPGKVW
                     AYALQMHRLSDAGVTKSMVLFANLASLAISLATSTLVGLLCLGISSAQGELRPLLLTA
                     FLGLGAIYSLATVFHAHPLRWLAQLLGRAFKRKFEYFSFDAAALLRLNAAHASAAVLS
                     GFSTYLACQGIGYAVTWDQAMTIMASGLIGDVVGFLAIVVPGGFGVREGVMWWLLRGA
                     TPGPVALILPIVSRLLSMGVELMLGVIALALLKRLNRERISVPVGAPGSNDELGS"
     gene            complement(1989..2906)
                     /locus_tag="VFQ61_30685"
     CDS             complement(1989..2906)
                     /locus_tag="VFQ61_30685"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9958911.1"
                     /translation="MASSQLLEILHRALPGGRVLTTQDLAGGVSARAVRVNVLLTGAG
                     DAKQFVVRRPSAANRSDASRIAEREYRLLSFLWTNRVRVPRPVALDLEAAAVVLEYVP
                     GSPDFVFPANGASLSEMADQLAAIHRLPIDALQFLPRREQTIEQQLSETDRELDRSLG
                     EATVRTALETWWPRHDTGPQSLLHGDYWPGNLLWNAGELSAVLDWEEAEIGNPLADLA
                     VTRLDLWWAFGADAAGIFTARYAETSHIDPRQLAIWDLCAALRPMSNLELWASCYAHP
                     PISRPDVTLEHMRTVHARFVAQALAALGD"
     gene            3028..3393
                     /locus_tag="VFQ61_30690"
     CDS             3028..3393
                     /locus_tag="VFQ61_30690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HET9958912.1"
                     /translation="MAKLITSPTRIEAAGNVPKIIEEFVGRVNNAESRLSIARMRSPQ
                     GWQEPGQRPEFDEYTVVLGGELRVDTERESFDVRAGQAILVKAGEWVRYSSPASGGAE
                     YIAVCLPAFSPDTVHRDAT"
     gene            4631..6232
                     /locus_tag="VFQ61_30695"
     CDS             4631..6232
                     /locus_tag="VFQ61_30695"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HET9958913.1"
                     /translation="MKLRRSVVYPISFSMLSWFAAGCSGTDLGGLGGLGSLPIGTGGS
                     TSAVPGATGGAPAAGSPGSGAVSSGATSSGGSVAQGGSGNTGPGPSRGGTTGGNNPPP
                     GGTGATSSSSGGSNPGTGTAGPRVDNPYAGVKGYVNPEWKAKADAEPGGSRVSDNPTG
                     VWMDRIAAIAGTGSSSSMGLRDHLDAALAQGAGYIQVVIYNLPGRDCAALSSNGELGP
                     TEIDRYQHEYIDPIAEIMSDAKYQSLRLINIIEIDSLPNLVTNTGSSKSATAACDVMK
                     ANGNYQKGIQYALGKLHKIPNTYNYVDAGHHGWIGWEENFNNSAQLFAETAKGAEGGV
                     ETVQGFITNTANYSALQEPYIKVTEQTRLSKWIDYNQYVDELSFAKAFRSKLVSLGFS
                     SSIGMLIDTSRNGWGGPNRPTQASTASDVNTMIDESRIDRRFHLGNWCNQAGAGLGER
                     PKVVGSDGIDAYVWIKPPGESDGASMAVENDQGKGVDGMCDPTYGGNARNGNNKSGAL
                     GSAPLSGAWFSAQFRELMDNAYPPL"
     gene            complement(6405..6893)
                     /locus_tag="VFQ61_30700"
     CDS             complement(6405..6893)
                     /locus_tag="VFQ61_30700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958914.1"
                     /translation="MTPSRSRKVLSYWTAFKRGNCDDPGTPGTHEVGVGVVPGVPGVL
                     GLPGVPGLPGVPGFPGVAAPPLIAPAPGAAPAPGEAPAPEGPGLFEPPGSSMVPEQPV
                     KSVTATAILLVHVCIPLTPAFLSTGIFRRERFGACLLLSMTQVQSHGTRGAVHISNDK
                     DQ"
     gene            6871..8328
                     /locus_tag="VFQ61_30705"
     CDS             6871..8328
                     /locus_tag="VFQ61_30705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9958915.1"
                     /translation="MRDLLGVTDHPSALLAPDGPGSVDQRAWDGYQAAAAAVAEQLKA
                     NTAVRAKVVTCSPSGDGSACAQQIIQNFGQRAFRRPLTAEETSRFMQLYTQRAELTAT
                     GSFDEAIQLIVRGFLLSPSFLTRAEISEATEADHYVLNGYEVASRLSYMLWGSMPDDA
                     LFAKAASGALGSPDGILTEAKRMLVDPKARARVTAFHEDYLHISANSRWTQVARDAAI
                     FPAFNPTVVPLLAEEARRFFEYITFDQNGTFQDLVLKPVAFVNKSLAPIYGLDPSKFG
                     TELTRTDLDPAQRAGFLTHAGFLTAFSSFDRTSPILRGAFVQKEVLCSDIPAPPADAT
                     SRVLPAVGATQRERVTAQTASEACLGCHQTVINPTGFALENYDGIGTYQTVEKGTGAT
                     IDTKVTVPIGRNAVEVTGAVDLMNKIAASPEAQACYAKKWVKFAYDRDLTSQDLCTVQ
                     SLATKMTQSGYTVLNLITDLTQSQSFRYRTPEVTP"
BASE COUNT         1587 a         2685 c         2584 g         1618 t
ORIGIN      
        1 ctcctgagtc caacgattcg ccgcttcgat atcctcgtac tcaatttcga gcgaatcaac
       61 ttgttcctcg aaaacttcca caagctacgg aacttcgatc cagagcgcga ccgcatcgtc
      121 atttcagatt gctccaacaa cgccggccga gaacgagagc gcgtggctcg cttcgcaaat
      181 gagcggggct ggatcttggg tggcccgcaa atcacgctgt cgagaagacc aaactggggt
      241 ttggcagagg gcgcccgcat cgactacttc actcagctgc tccgcgacga tactgtcgcc
      301 ccatacgttt ggcaattcca ggagcactat ctcgatacga cgtcggacta cagccgttgg
      361 cccgcttcgg accgcgctcg ggcgggacaa gtcaaagcgg acatcctgcc ggacggaatg
      421 gtcatcgatc tggacatttg cgagcaggct ttctcgaacc ccgacgtagc agtcctctgt
      481 gcgccccgat gtggactcgg catcttctct catcccgatg ggcgggagtg gttatatgcc
      541 gacggcgcca acttcggttt taggaccagc gtcgcgcgcc gagtgctttc gaaccagctg
      601 ctcgatgatt atcggttgac cttcgacgcg tcctaccgtt gggcgatttt catggaattc
      661 gagtactcgc ggctattctc tgagggcgcc tggttcgacc tagtgcacga gatggctttc
      721 cgtgacgtgg cggaagttag ggccgcggag caacagctcg gcaaaccgat ctccaccatt
      781 gcggaagagt ctagcgcccg ccggcaccaa cggtacgagc gccgcgcgcg actcgtcaac
      841 aagtttcccg agctgttgcg gcaggggcta cttgcgactc acttcaaggc gctgggcatc
      901 gcacaaacca agttgatcgg cccatcacgc gctaagctgc tggcgatgaa catcgattcg
      961 ccgcagtggt tccgcgatgt ctggtgagcg cccccggaag attgttcgaa atctgatcgg
     1021 cgcggcaatc atagcctgcg tcgcttactt cttccttcgc gcgctccgcg acaattggga
     1081 ccgactgcgc gctctcgagt tcgaagccca ttacgcggcg ctggccctga ccgccctttc
     1141 gatagcgggt tccaatctcc ttggcaccgc ggcttggcaa gccgccatga acaccttcgc
     1201 gaagcgcccc ttgagcttct ccgagagcat cgccaccgtc aacatctcga gtttaaccaa
     1261 gtacgtgcct ggcaaagtat gggcctacgc gctgcaaatg caccgcttgt ccgatgccgg
     1321 tgtcaccaag agtatggtgc tcttcgccaa cctagcgagc ttggccatct cgctcgcaac
     1381 ctcgactctg gtcgggttgt tgtgtctcgg gatctcctcg gcccaggggg aactgcgacc
     1441 actcctcctg acagcatttt tggggctcgg ggcgatctat tcgctcgcca ccgtttttca
     1501 tgcgcatccc ctgcgttggc tggctcaact actaggtcga gccttcaaac ggaaattcga
     1561 atacttctcg ttcgacgcgg ccgcattgct gcgtttgaat gccgctcacg ccagtgcggc
     1621 tgtgctctcg ggattcagta cgtacttggc gtgtcagggc attggttatg ccgtcacgtg
     1681 ggaccaagcc atgacgatta tggcctctgg cctgatcggc gacgtagtcg gcttcttggc
     1741 gatcgtggtt cccgggggct tcggtgtgcg cgaaggggtg atgtggtggc tacttagggg
     1801 cgcgacgccc ggcccggtcg cgttgattct ccccattgtt tcgcggctac tctcgatggg
     1861 tgtagagctg atgctcgggg tgatagcctt ggcgctactc aagcgattga accgcgaacg
     1921 catcagcgtt cccgtgggtg ctccgggttc gaacgatgag ctcgggtctt agtcgcggaa
     1981 actttcgctc aatctcccaa agccgcgagg gcttgcgcca cgaaccgtgc gtgtaccgtg
     2041 cgcatatgct cgagggtgac gtccggtcgt gaaattggtg ggtgggcgta gcaagaagcc
     2101 caaagctcca agttggacat cgggcgtagg gctgcgcaca agtcccagat tgcgagttgc
     2161 cgaggatcga tatggctcgt ttcggcataa cgagccgtga atatcccggc cgcgtccgct
     2221 ccgaacgccc accagaggtc caatcgggtt acggccaaat ctgccaatgg gttgccgatc
     2281 tcggcctctt cccagtccag taccgcgctg agttcgccgg cattccacag caagttgccc
     2341 ggccaatagt cgccatggag caacgattgc ggcccggtat cgtggcgcgg ccaccaagtc
     2401 tcgagtgcgg tccgcacggt cgcttctccc aagctccgat cgagctctcg gtccgtttcc
     2461 gagagctgct gctcgatggt ctgctctcgc cgcggcaaga actgcaatgc atcgatggga
     2521 aggcgatgaa ttgccgccag ttgatcggcc atctccgaca aagacgcgcc gttggccgga
     2581 aagacgaagt ctggcgaccc cgggacgtac tccagcacta ccgcggcggc ctccaaatcg
     2641 agagctaccg ggcggggcac gcgcacgcgg ttcgtccaca agaacgacag cagacgatac
     2701 tctcgctcgg cgattcgact cgcgtcgctc cggttcgcgg cgctcggccg acgcaccacg
     2761 aactgtttgg catcccccgc gcccgtgagc agtacgttca cgcgaactgc gcgggcagat
     2821 acccctcccg ccaggtcctg cgtggtcaga acgcggccgc ccggcaacgc ccggtgcagg
     2881 atctcgagga gctgtgagct agccatgcgc tcgtccagga tacgatgacg cggcgccgag
     2941 cgctcctgaa cgcgtcctca ggcaagcacc ccagccgccg gagcgattgg tcatgctgga
     3001 gcacggtttc ggtttatcct gctccgcatg gccaaactga tcactagccc cacgcggatc
     3061 gaagccgccg ggaacgtgcc caagatcatc gaggagttcg tgggtcgcgt gaacaacgcc
     3121 gagtcccgcc tcagcatcgc gcgcatgcgc tcgccgcaag gctggcaaga acccggacag
     3181 cgtccggagt tcgatgagta tacggtcgtg ctaggcgggg agctgcgggt ggacaccgaa
     3241 cgggagtctt tcgatgtgcg cgcggggcag gcgatcctgg tcaaggccgg agaatgggtg
     3301 cggtatagct ccccggcctc aggcggagcc gagtacattg cggtgtgcct gcccgcgttc
     3361 agtccggaca cggttcatcg cgacgctacc tgacgggagc gcatcgtttc agggtcgtag
     3421 caataagcac tgacactcat tccgtgtttg ttctccgcga acatcgaatg agtgcaggct
     3481 ccgaggcgtc gtctctcgtg gtgtctagtt actcttatcg cagagcctcg atgcgctggt
     3541 gtaattctgt ttctgcgtgc gaacgattcc gaatctgttt acgcgtaaca cctgttcgaa
     3601 gcggtgcgag ccgagctgga gtatcggcgg gggggcaaag gacgcgcttg gctcaggcgt
     3661 ttgcggcaaa agtctcgccg agaatgccct aaccggagac gtctccgccg actggcgagc
     3721 tgaactcggt gcggtgaggc accggttcca gacgtgcggt ctctggccta gatggagcga
     3781 tccgcggcgt ttgctcgcgc tgcgccgcag acaacgcgag cgccaaattc cagagagacg
     3841 cgcgcccgtg cgggctgtgg cgaggagcgg gattttcggg tggttcgcgt ctggtagcgg
     3901 ttccagaaat tggtggaggt ggcagtgccg tcgcgcccac gggcgcggct ccacggcgtc
     3961 ttctttgctc gaaaattgcc gaatatcatg ggcacgctcg ccgccaatga tcgctttgca
     4021 tgcccgcgcg gcgagcccag gctgcccgac gatttgtcga aaatcccgcg gaaaatggcg
     4081 cgtgctgccc gcagtcgaca gggcgcgccg cactttcgtg ctgattcacg tcactgccaa
     4141 gtacggtgaa tccacggtca ccctgcgtgt ctcgccttga ctcggtgttt ggtgagggct
     4201 aatgaaggtg cccagttcgc aacccattgg tgttgcgtat ttacgaattc cgcgcaaggt
     4261 cggctggcga gctcgaccgt gctcggtgcg atagtccgct ttcttcaagc tgccccgtcg
     4321 tggggtggtc gtggtcgagt caccgtatgg tcgaggcctc ggtcgaaaca ccagactcgt
     4381 ggcttcggac cagcagagca tcgagctcgt tggggccttc atctatggcc cctcataagg
     4441 gcctccgcat gaggcctttc gcccaacagc gtttcgccgg gaggccctcc gccaacgggc
     4501 agaacgctac agcaccctaa gaccgcccgc actgtctggc tcgctgaatt ccggtcgtct
     4561 cgaagatggc tttggtcacg agtccgcgca gagccgcgcg ctgaattcgt accgacgaaa
     4621 gagagaaacc atgaaattgc gcagaagcgt cgtctacccg atctccttct ccatgctgag
     4681 ctggtttgcc gcaggttgct cagggactga cttgggcggc ctgggtggac tcggctcatt
     4741 gccaatcggc accggcggca gcacgagcgc agtgcccggc gcgacgggtg gcgccccggc
     4801 tgcgggcagc cccgggagcg gcgccgtatc ttcaggggcc acgagctcgg gtggttcagt
     4861 ggctcagggc ggcagtggca acaccggtcc tggcccgagt cgcggtggca ccactggtgg
     4921 caataaccct cctcccggcg gcaccggcgc aacttccagt tcgagcggcg gcagcaaccc
     4981 cggtacgggt accgcgggtc cgcgcgtcga caacccgtac gcgggtgtga aaggttacgt
     5041 caaccctgaa tggaaggcga aagccgacgc agagcctggt ggcagccgcg tctccgacaa
     5101 tccgactggc gtatggatgg accggattgc ggccatcgcc ggcacgggct cgagctccag
     5161 tatggggctg cgcgatcatt tggacgccgc tctggcgcaa ggcgccggct acatccaggt
     5221 ggtgatttac aatctacccg gtcgcgactg cgccgcgctc tcctcgaacg gcgaactcgg
     5281 tcctacggag atcgatcgct accagcacga atacatcgat ccgatcgcgg aaatcatgag
     5341 cgacgcgaag taccagtcgc tgcgcctcat caacatcatc gagatcgact cgctgccgaa
     5401 tctggtgacc aacaccggca gttccaagtc cgctacggcc gcgtgcgacg tgatgaaggc
     5461 caatggcaat taccagaagg gtattcagta cgccctcggt aagctgcaca agatccccaa
     5521 cacctacaac tacgtggacg cgggtcacca cggttggatt ggctgggagg agaacttcaa
     5581 caactccgcc caattgtttg ccgaaacggc caagggcgcc gagggcggcg tcgagaccgt
     5641 gcaaggcttc atcaccaaca ccgcgaacta ttcggcgttg caagagccat atatcaaggt
     5701 cacggagcag acccgactct ccaagtggat cgactacaac cagtacgtgg acgaactctc
     5761 gttcgcgaag gcgttccgca gcaagctcgt ctcgctcggg ttcagctcca gcatcggcat
     5821 gctgatcgac acctcacgca acggctgggg cggcccgaat cgccccactc aggcgagcac
     5881 tgccagcgac gtcaacacga tgatcgacga gtcccgcatc gaccgccgct tccacctcgg
     5941 aaactggtgc aatcaagcgg gcgcgggcct cggtgagcgt cccaaagtgg tcggctcgga
     6001 cggcatcgat gcctacgtct ggatcaagcc gccaggtgag tcggacggcg cgagcatggc
     6061 cgtggagaac gatcaaggta agggcgtcga cggcatgtgc gatcccactt acggaggcaa
     6121 cgctcgaaac ggcaacaaca agagcggcgc gctcggcagt gctccgctct cgggagcttg
     6181 gttctccgct cagttccgcg agctgatgga caacgcgtac ccgccgctct gagcgtccgt
     6241 ctagtcccgc cgcgaattcg taaaaacggt tagttcaact gaagaggtcg ctaggtacca
     6301 cctagcggcc tcttcgcgtt ttgagcgaac agacgcagta acgctcgatg ccggagggca
     6361 ggcgcaattt cgtaacttgc gtctcgtatt tccaaaaacg aacgttactg gtctttgtca
     6421 ttcgaaatgt gaaccgcccc gcgtgtgccg tgactctgca cctgagtcat ggagagcagc
     6481 aggcacgcac caaaacgctc tctccggaaa attcccgtac tcagaaaggc cggggtgagc
     6541 ggtatgcaca cgtggaccag aagaatcgct gtcgcggtca cgctctttac gggctgctcc
     6601 gggaccatcg aagatccagg cggctcaaag agcccagggc cctctggggc gggtgcttcg
     6661 ccgggggcgg gagccgcgcc cggcgcaggc gcaatcaacg gtggcgccgc aacaccgggg
     6721 aatcccggta caccaggaag ccccgggaca cccggtaatc cgagcacacc aggcaccccc
     6781 ggaaccacgc cgactcccac ctcgtgtgtg ccgggagtcc cggggtcgtc gcagttgccg
     6841 cgcttgaacg ccgtccaata cgataacacc ttgcgcgatc tactcggtgt caccgatcac
     6901 ccttcagcgt tgttggctcc ggacgggccg ggcagcgtgg atcagcgtgc gtgggatggt
     6961 taccaggccg ccgccgctgc cgttgccgag cagctgaaag ccaacacggc cgtacgcgcc
     7021 aaagtcgtga cttgctctcc gagtggagac ggcagcgcgt gcgcccagca aatcattcag
     7081 aacttcgggc agcgcgcgtt ccgccgtccg ctcaccgccg aagaaacgag ccgcttcatg
     7141 cagctctaca cgcagcgcgc ggagctcacc gcgacgggta gcttcgacga agccatccag
     7201 ctcatcgtgc gcggcttcct actctctcca tcgttcctca cgcgcgccga gatctccgag
     7261 gcgaccgagg cagatcatta cgtgctcaac ggttatgagg tcgcctcccg cctctcgtac
     7321 atgctgtggg gcagcatgcc ggacgatgcg ctgttcgcga aggcagcctc cggcgcattg
     7381 ggtagccccg atggcattct gaccgaagcc aagcgcatgc tggtcgatcc caaggcgcgc
     7441 gcccgtgtga cggcgtttca cgaggactac ctgcacatca gcgctaactc acgctggact
     7501 caagtggcgc gggacgccgc catcttcccg gcgttcaatc caacggtcgt tcctcttctc
     7561 gccgaggaag cgcggcgctt cttcgaatac atcaccttcg atcagaacgg cactttccag
     7621 gacttggtgc tcaagcccgt cgccttcgtg aacaagagct tggcgccgat ttacgggctc
     7681 gatcccagca agttcgggac cgagctcacg cggaccgatc tcgaccctgc ccagcgtgcc
     7741 gggtttttga cccacgccgg tttcctgacg gcgttctcct ccttcgatcg gacctcaccg
     7801 attctgcgtg gcgccttcgt gcagaaggag gtgctttgct ctgacatccc ggctccaccg
     7861 gcggacgcca cctcacgggt gctgccggcg gtcggcgcaa cgcaacgcga acgggtcacc
     7921 gctcaaaccg cgtccgaagc gtgcctcggt tgccaccaaa ccgtgatcaa tccgaccggc
     7981 tttgcgctcg agaactacga cggcattggc acgtaccaaa ccgtcgagaa ggggaccggg
     8041 gccacgattg acaccaaggt taccgtccca atcggccgca acgcggtcga ggtaaccggc
     8101 gcggtagatc tgatgaacaa gatcgcagcc tcgccggaag ctcaggcctg ctacgccaag
     8161 aaatgggtga agttcgccta cgaccgcgat ctcaccagcc aggatctgtg caccgtgcag
     8221 agcctcgcca cgaagatgac ccagtctggt tacacggtct tgaacctgat taccgatctg
     8281 acccagagcc aatctttccg ctaccgcact ccggaggtga cgccgtgaat cgtagaaagt
     8341 tcctgctcgg cgtgggtggc gcggcactcg cgaccccgtt cttgagctcg ctcgagccac
     8401 gcaccgcacg cgctcagacg gctgcttcgc ccaagcggct cgtgatcttc tatacccaga
     8461 atggctgtct cacg
//
LOCUS       DASTKU010000872         6692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_839397, whole genome shotgun sequence.
ACCESSION   DASTKU010000872 DASTKU010000000
VERSION     DASTKU010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839397"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="pstA"
                     /locus_tag="VFQ61_30710"
     CDS             complement(<1..663)
                     /gene="pstA"
                     /locus_tag="VFQ61_30710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET9958916.1"
                     /translation="MFTETALGKAKARNERIAQFVFGGMAMAMVVPLLLIVGYLVWQA
                     WPMLSIDFLTSNPTGGMRRGGIWSALLGTIYLVGVSLLIAAPVGVLAAVYLNEYAQDN
                     WLTRIINLAVVNLAGVPSIVHALFGLGAFVLFAGIGRSILAASLTLAVMTLPVIIAST
                     KEALAAVPRTFREACWNVGATRWQTIRGIVLPNSVSGILPGVSLQVSRAAGETAPIMF
                     TGA"
     gene            complement(702..1676)
                     /gene="pstC"
                     /locus_tag="VFQ61_30715"
     CDS             complement(702..1676)
                     /gene="pstC"
                     /locus_tag="VFQ61_30715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET9958917.1"
                     /translation="MEEEVARFAAPPVQAGPASLRMSQSRNVARRRKLEALTELGIEW
                     LIRFCGISAILFVFGIFFFVMREGVGLLFGDFSITKFLFTTEWYPTSHSNVRYGVLAL
                     IVGTASVTMTAMLIAVPFGLGAAIFVSEFCGRRTKEWLKVVIELLAAIPSVVWGFIGI
                     SVMNPLIQDVFHVPVGLNVLNGALLLALMSVPIMVSIGEDALKAVPDSFREAAVAMGA
                     TRWQVVYRVLIPAAKNGLLAAVLLGVGRAVGETMAVLMATGHAVNIPHSVFDPVRTLT
                     ATIAAELGEASVGSSHYQVLFLVGMLLFSITFVVNLTADLIVRGVRKG"
     gene            complement(1716..2672)
                     /locus_tag="VFQ61_30720"
     CDS             complement(1716..2672)
                     /locus_tag="VFQ61_30720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET9958918.1"
                     /translation="MSEVTVSAGRHASSWVRVGRGLLLGLCVAALSVGCKKKTEPESG
                     STTAAVEGKKESIQNIGSDTMVNLAQAWAEEYAKVQPNVSVEVSGGGSGVGIAALING
                     TANIANASRKLEPEEAEKAKQKGHEPKEFMVGYDGLAIYVHKSNPIQEISVEELSEIY
                     REGGKIAKWSDLGVKEIPGAKDGSIIRVSRQNNSGTYHYFRETVVGKKNDFKAGSLDM
                     NGSKDVVELVAKTPGAIGYSGLGYATAEVKIVKVSKKKGEPAVMPSIATVLDKSYPIS
                     RPMFMYTPGEPDAAVKKYIDWVMSEAGQKIVESTGYVPLTHK"
     gene            complement(2686..3783)
                     /gene="phoU"
                     /locus_tag="VFQ61_30725"
     CDS             complement(2686..3783)
                     /gene="phoU"
                     /locus_tag="VFQ61_30725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET9958919.1"
                     /translation="MTHFEETLQRDVERIRSKVREMATLAEGSLNGCMSALVGRNRQI
                     AYSVILRDQRIDELEKEIDRLCLEFIVRQQPVAGTLRFAYAVIKISSELERVGDYAES
                     MARQGLSLLAMDLQFPMERLQEIAGKAIPMLHGAVEAFVTQDADGAKKTMETEEVVDR
                     LRHELGSEIMSRIQSGNLPLEAYTPLMNIVNRLERVADQARSICQQTLYMCTGEYSKH
                     AGGEVFRLLFVDDNDSCRSQMAEAIGSLMQQPKFVFSSAGLERHPIDSFTVQFLRDKG
                     IDISRNVSKAVDQIPHLDHYQIIVALSKDAQRVFPPPPTKAVCLDWSIPDPSLVKGSP
                     EEVRAAYEETYKFLKVHIRDLVQAILGDQSS"
     gene            4187..4525
                     /locus_tag="VFQ61_30730"
     CDS             4187..4525
                     /locus_tag="VFQ61_30730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958920.1"
                     /translation="MVRVAIRGCYTPKDGEAIFSLLERYSEHPEPPSKFYFDLGDMTE
                     FGLGYLDVVQQAKRAMQLRFTRRLVFGIYTTSPVTRGFATVFGKFVDKLKLRVVVSDD
                     REIVERELRF"
     gene            4718..5710
                     /locus_tag="VFQ61_30735"
     CDS             4718..5710
                     /locus_tag="VFQ61_30735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958921.1"
                     /translation="MVAIKLVRGSARENAPNRDTCVKRGLEFLASRAALFFPAYLSDT
                     SDFEKRLETPPEVFTPALILNALSECGIEPDFLEDVVDSLMEAFTDRGLVHFFRDRDL
                     LPADIDCTAVLLETMHRYQRVLKFEVDGALSRVARNRDDQGVLRVYIDPDPSRVDRLD
                     AAACANALYTFELFETVVDARPTLEYIEKYLRSDDFEQGTRYYPSPDVILMFLSRWLR
                     DAASAPQPLRELARLRTSDRLRQTTGNALDLAARVTAANNLGLSSGQALERLISLQRS
                     DGSWPAAPCFKFGRNVRYFGSESLTTAFALRALKLNTYMRRQSGTFLKSTVSTP"
     gene            5707..6675
                     /locus_tag="VFQ61_30740"
     CDS             5707..6675
                     /locus_tag="VFQ61_30740"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010333 - terpene synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0046246 - terpene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terpene synthase family protein"
                     /protein_id="HET9958922.1"
                     /translation="MSTRERTTEPSQFSLDYVYPMPHFELTLEEKRQVRAVLEVLAER
                     YALFESRAAWTHVLEVFIANCSARRRDPECLGLLCHFLYMTLYFNEARSHGVSPELLR
                     AYLRVMRGGTSAPLHPVLRAAAEFKPKLDQYLRDAQLRGKDCDPSGFYHYLGLNLSGF
                     ALDAAASRGAPSAGETQAPDVESYAAVRLHTISALGYFHFWKLLLQVEADAELGSGAL
                     LFRCELLSARIQAMANDLASLARDNHDRSNNWVNVVAREQRLSQEAAFVRVSEEHASC
                     VSRYSTAAQQARSAIDDTSTLDYLELIGSCTVGNLKSMRALQQRYA"
BASE COUNT         1255 a         2118 c         2008 g         1311 t
ORIGIN      
        1 ggcgccggtg aacataatcg gcgcggtttc gcccgccgcg cgggatactt ggagactcac
       61 gcccggcagg attccgctca ccgagttggg taggacgatg cctcgaatgg tttgccaacg
      121 cgtagctccg acgttccaac acgcctcgcg gaaagttcgc ggtacggctg cgagcgcctc
      181 cttggtgcta gcgatgatta ccggtaaggt catgaccgcg agggtcagcg acgccgccaa
      241 gatcgagcgc ccaatccccg cgaacagcac gaaggcaccc agaccgaaca gcgcgtggac
      301 gatactcggt acgcccgcga ggttgacgac ggccagattg atgatgcggg tcaaccagtt
      361 gtcctgcgcg tattcgttca ggtaaaccgc cgccaaaact ccgaccggag cggcgatcaa
      421 cagcgataca cccaccaagt agatcgtgcc gagcaaggcc gaccagatgc caccccgccg
      481 catgccgccg gtcgggttgc tggtgaggaa atcaatcgag agcatgggcc acgcttgcca
      541 cacgaggtag cccacgatta ggagcagcgg gaccaccatg gccatcgcca tgccgccaaa
      601 cacgaactgt gcgatacgct cgtttcgagc cttggccttg ccgagcgcgg tttcggtaaa
      661 catcacgacc tcttctcgag tcgcttgact cagctccagc ttcagccttt gcgcacaccg
      721 cgtacgatca gatccgccgt gaggttcacg acgaaggtga tcgagaacag cagcatgccc
      781 accaagaaca acacctggta gtgcgaagaa cctacggaag cctcaccgag ctccgcggcg
      841 atcgtcgcgg tcaacgtgcg gaccggatcg aacacgctgt gcgggatatt cacggcgtgc
      901 ccggtggcca tcaacacggc catggtctcc cccactgcgc gcccgacccc gagcaacacc
      961 gccgcgagta ggccgttctt cgccgctgga atcagcactc ggtaaaccac ctgccaacgc
     1021 gtggcgccca tcgccaccgc cgcctcgcgg aaagaatcgg gcactgcctt gagcgcgtct
     1081 tctccgatgc tgaccatgat cggcacgctc atcaacgcca gcagcaacgc gccgttcaac
     1141 acgttgagcc ccaccgggac gtggaagacg tcctgaatca gcgggttcat gacgctgatt
     1201 ccgatgaagc cccagaccac cgacgggatg gccgcgagta gctcgatgac gaccttgagc
     1261 cactccttgg tgcgccgtcc gcagaactcg gagacgaaga ttgcagcccc caggccgaag
     1321 ggcaccgcga tcaacatcgc cgtcatcgtc accgacgcgg tgcccacgat cagggccagc
     1381 acgccgtagc gcacgttgct gtgcgaggtc gggtaccact ccgtcgtgaa caagaacttg
     1441 gtgatgctga agtcgccgaa caggagcccg acgccttcac gcatcacgaa gaagaagatg
     1501 ccgaaaacga agaggatcgc gctgatcccg cagaagcgaa tcaaccactc gatacctagc
     1561 tccgtgagcg cctccaactt gcggcgccga gccacgttac gcgactgcga catgcgcagc
     1621 gaggcgggtc cggcctgcac ggggggagct gcgaatcgtg ccacttcttc ttccataggg
     1681 cctcctagcg cgagggccga tcactcgggt tcgactcact tgtgggtgag cggaacgtaa
     1741 ccggtactct ccacaatctt ttgaccagct tctgacatca cccaatcgat gtacttcttg
     1801 acggccgcgt ccggttcgcc cggggtgtac atgaacatcg ggcgagaaat ggggtagctc
     1861 ttgtcgagca cggtcgcgat gctgggcatc acggcgggct cccctttctt cttcgagacc
     1921 tttacgatct tcacttcggc cgtggcgtac ccaagtccgc tgtatccgat cgcgccgggc
     1981 gtcttggcca cgagctcgac cacgtccttg gatccgttca tgtcgagcga gcccgccttg
     2041 aagtcgttct ttttgccgac cacggtctcg cggaaataat ggtaggtgcc ggagttgttc
     2101 tggcgactaa cgcggatgat cgagccatcc ttggcgcccg gaatctcctt gactcccaga
     2161 tcggaccact tggcgatctt gccgccctct cgatagatct ccgagagctc ttcgaccgaa
     2221 atctcttgga tcgggttgct cttgtggacg taaatcgcga gaccgtcgta gccgaccatg
     2281 aactccttcg gctcgtggcc tttttgcttg gctttctccg cttcctcggg ctcgagcttg
     2341 cggctggcat tcgcgatgtt ggcggtgccg ttgatgagcg cggcgattcc gacgcccgat
     2401 ccaccacccg aaacctctac cgagacgttg ggctggacct tggcgtattc ctcggcccag
     2461 gcctgagcga ggttcaccat ggtatcggaa ccgatgttct gaatcgattc cttcttaccc
     2521 tcgacggccg cagtggtact gccgctctcc ggctccgtct tcttcttaca gccaacgctc
     2581 agcgcggcca cacacaagcc caacagcaaa ccgcgtccca cgcggaccca cgaactcgcg
     2641 tgccgacctg ctgataccgt aacttcactc attcgtattg ttccttcagc tgctctggtc
     2701 acccaggatg gcctggacca ggtcgcgaat gtgcactttc aaaaacttgt acgtctcctc
     2761 gtaagccgcg cgcacttcct cggggctgcc cttcacgagc gaaggatccg ggatgctcca
     2821 gtccaaacac accgccttgg tcggtggcgg cgggaacacg cgctgcgcgt ctttcgagag
     2881 cgcgacgatg atttgatagt gatccaggtg tgggatctga tcgacggcct tcgacacgtt
     2941 gcgggatatg tcgatgccct tgtcgcgcag gaactggacc gtgaacgaat cgatgggatg
     3001 tcgctcgagc cccgcgctcg agaagacgaa cttcggttgc tgcatcaagc taccaatcgc
     3061 ctccgccatc tgactgcggc aggagtcgtt gtcgtccacg aacaacagcc gaaacacctc
     3121 tccgccggcg tgcttggagt attcacccgt gcacatgtag agcgtctgct ggcagatgct
     3181 gcgcgcttga tcggccacac gctccagccg attcacgatg ttcatgagcg gcgtgtacgc
     3241 ctcgagcggc aggttgccgc tctgaatgcg cgacatgatc tccgagccga gctcgtgacg
     3301 gaggcgatcg acgacctcct cggtctccat ggttttcttg gccccgtccg cgtcttgggt
     3361 gacgaaggcc tccaccgcgc cgtgcagcat cgggatcgcc ttgccggcga tttcctgcaa
     3421 acgctccatc ggaaactgca agtccatggc caaaagcgac aggccctgcc gcgccatgct
     3481 ctcggcgtaa tcccccacgc gttcgagctc cgagctgatc ttgatcacgg cgtaggcgaa
     3541 acgcagcgtg ccggctacgg gctgttgccg aacgatgaac tcgaggcaaa ggcgatcgat
     3601 ctccttctcg agctcgtcga tacgttgatc ccggaggatc acggagtacg cgatttgtcg
     3661 gtttctaccg accagcgcgg acatgcagcc gttgagagag ccctccgcga gcgtggccat
     3721 ctctctcact ttcgagcgaa ttcgctcgac gtcccgttgt agagtctcct cgaagtgcgt
     3781 cactggtgcg gccttgctgg tgctgccgta ctctccgggg cgcgagtgac attcgcgtga
     3841 cgcacccgtt acatgtgtga caacctagcc gtccaagtag cggctccgtg acgctggcgg
     3901 ctctgtctcg taacgtcgga gcgggtaagt tcccagtacg cgcgggcctg ccacagccac
     3961 cctcccccag cggaggttcg acgccgaacc gctgcggacc ggaaacataa gcctaggcat
     4021 cctgcgcgtg accgaatcga aattcggctc gaacgggcgg ttcgtctctg aacggagcgc
     4081 ggcacacggc cgtacctggc tgcgtaggcc gctattcctt cgcggcccca ccgcaacgcc
     4141 gcaccttggc ccgaaaatgc acacgatagt ctccgagaat tcgcaattgg tgagggtagc
     4201 catccgcggc tgctacacgc caaaggatgg ggaagcgatt ttctcattgc tcgagaggta
     4261 cagcgagcac cccgagccgc cgtcgaagtt ctacttcgac ttgggcgaca tgaccgagtt
     4321 cggcttgggg tacctcgatg tcgtgcaaca ggcgaagcga gcgatgcagc tgcgttttac
     4381 ccgtcgtctg gtgttcggga tctacaccac gagcccagta acccgtggct ttgccaccgt
     4441 gttcggaaaa ttcgtggata agttgaagct gcgcgtcgtg gtctcggacg accgtgaaat
     4501 cgtggagcgc gagctccgct tctgagacgc gcaagccggc ctgcatcgcg gccgtgagac
     4561 tactcactag ggttgttctg ctcacccggt gagctgtcca accatccttt cgctcaaggg
     4621 atctcaggcg gatgacttaa ggggcgctcg cgggcgccgt tgcgtggagt gtgtcccgca
     4681 cgcggcgagg agcggtcggg cacaagggag gctcggcatg gttgccatca agctggtccg
     4741 gggatcggcg cgcgaaaatg cgcccaatcg agacacttgc gtcaaacgtg ggctggagtt
     4801 tctagcgtcc cgagcggcgc tatttttccc agcgtatctg tccgacacga gcgacttcga
     4861 gaagcggctc gagacaccgc cggaggtctt cactccggcg ctgattctca atgcgctctc
     4921 ggagtgcggg atcgaaccgg actttctcga ggatgtggtg gatagcttga tggaggcctt
     4981 caccgatcgt ggactggtgc atttcttcag ggatcgcgac ctgctacccg cagatataga
     5041 ttgtacggcc gtgctgctcg agaccatgca ccgctatcag cgcgttttga aattcgaggt
     5101 ggacggcgcg ctctctcgag tggctcgaaa ccgcgacgat caaggggttt tgcgcgtata
     5161 catcgatcca gatccgagcc gcgtggaccg cctcgacgcc gccgcctgtg cgaacgcgct
     5221 gtataccttc gagttattcg agaccgtggt ggatgcacgc cccacgctcg agtacatcga
     5281 aaagtatctg cgtagcgacg acttcgagca gggcactcga tactatccct cgcccgacgt
     5341 catcttgatg ttcctttcgc gctggctgcg cgatgccgcg agcgctccac agccgttgag
     5401 agagttggct cgtttgcgga ctagcgatcg cttgcgacag acgaccggca acgcgctaga
     5461 cctggccgcc cgcgtgacgg cggccaacaa cctgggtctc tcgagcggtc aggccttgga
     5521 acggctgatt tctctgcaac ggagcgatgg ctcgtggccg gccgcgccct gcttcaagtt
     5581 cggacgcaac gttcggtact tcggcagcga gagcctcacg accgcattcg ccttacgcgc
     5641 gctgaagcta aacacctaca tgagacgcca gagcggcacg ttcctcaagt cgacggtgtc
     5701 gaccccgtga gcaccaggga gcgcacgacc gagccctcgc agttcagcct cgactacgtc
     5761 tatcccatgc ctcacttcga gctcacgctc gaggagaaac gacaggtgcg ggcggttctg
     5821 gaggttcttg cagagcgtta cgcgctattc gaaagccgcg cagcttggac gcacgtgctc
     5881 gaggtcttca tcgccaactg ctcggctcgt cggagggacc cggagtgtct aggactgctg
     5941 tgccatttcc tgtacatgac gttgtacttc aatgaagcgc gtagccacgg cgtgtccccg
     6001 gagttgctgc gcgcttatct gcgggtaatg cgcggcggta cgagcgcgcc attgcatccg
     6061 gtgttgcgtg cggccgcaga gttcaagccc aagctcgacc agtacttgcg tgacgcgcaa
     6121 ctgcgcggca aggactgcga cccgtcgggg ttctaccact acttggggct caatctgtcc
     6181 ggcttcgcgc tcgacgccgc ggcctcacgg ggcgcgccgt cagcggggga aacgcaagct
     6241 ccggacgtgg agagttacgc cgcggttcgc ttgcacacga tatccgcgct cggctacttt
     6301 cacttttgga agctgttgct gcaggtggaa gctgacgcgg aactgggcag cggcgcactg
     6361 ctgttccgtt gcgagctctt gtctgctcgc attcaagcca tggcgaacga cttggcgtcg
     6421 ctcgcccggg acaatcacga ccgcagcaac aattgggtga acgtggtcgc gcgcgagcag
     6481 cgtttgagcc aggaagcggc tttcgtgcgt gtctcggaag aacacgcgtc gtgcgtgtcg
     6541 cgatattcga ccgccgccca gcaggcgcgt tcggccatcg atgatacgag cacgctcgat
     6601 tacctggagc tgattggctc ctgcacggtc gggaatctaa agagcatgcg ggctttgcag
     6661 cagcgctacg cctagcgccg ttcgatcggt cg
//
LOCUS       DASTKU010000873         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1287733, whole genome shotgun sequence.
ACCESSION   DASTKU010000873 DASTKU010000000
VERSION     DASTKU010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287733"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1696)
                     /locus_tag="VFQ61_30745"
     CDS             complement(113..1696)
                     /locus_tag="VFQ61_30745"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HET9958923.1"
                     /translation="MTLDLSKEKDPEVLRNAALLLERENQRLVSEVVKLTKQITELMG
                     GSGEDVQLRLQLLEVQLARAQKQLFGPSSERTRDDGSESSASDDQSPNDEAQADPKPQ
                     RGHGRREQSKLPRITKRHELVDADCACKVCGGRLEKFGDQADRSTEIHVIRRVFVNVE
                     HVQQKYRCPNNCGIETAPGPRKLFPRARYSIDFAIEVAVQKYLYHMPLARQVRQMRAE
                     GLEVDTQTLWDQIEKLATLVHPAYERIRQFVLEHAVVGADETTWRMMGKSDVEEGKTW
                     QVWTLAAPTAVYYKIESSRAHTVVLELFKGFRGIVLCDGYKGYTAAAKKLPGLRIANC
                     WAHVRREFKEVEKFFPAQTATVLSHINELFEIERLAIGDSPEARQARLALRTERSKPI
                     VEAIGKWAVEVTTMPGNALAKAIDYMTGVWSGLTLFLENPSVPLSNNITERANRDPVV
                     GRKNHYGSRSKRGTEVAAQLYTLIETAKMSGVDAHAYLSLAVHAALDGRALPLPHEVA
                     VEAAALIEQHFGAARASPI"
     gene            complement(1828..2193)
                     /gene="tnpB"
                     /locus_tag="VFQ61_30750"
     CDS             complement(1828..2193)
                     /gene="tnpB"
                     /locus_tag="VFQ61_30750"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET9958924.1"
                     /translation="MIGSGRRRRVFAYGSHCDMRKQYDSLAVLVTAAMGHDLLRGDLF
                     LFVARSRTRAKVLYFDGTGLCLLAKRLDRGHFAAPWEGRVEFTESELALFLEGAELVG
                     RVALSPSIVSPADRRVVFR"
BASE COUNT          491 a          914 c          889 g          568 t
ORIGIN      
        1 tacatgctcg cccaagcccg tcgtgaccag acacgggggt cgtaataggc gcgggcgccc
       61 gtcgcggacg cggaccgcgg gcgtctccgg gctgtgcggc tgtgcgtgag actcagattg
      121 gcgaggctcg ggcagcaccg aaatgctgct cgatgagtgc agccgcctcg accgcgactt
      181 cgtgaggtag tggcagcgcg cgaccgtcga gtgcggcgtg cacggcaagc gagagatagg
      241 cgtgcgcatc gacgccagac atcttcgcgg tctcgatcaa cgtgtacagc tgcgcggcga
      301 cttcggttcc gcgcttggag cgtgagccgt agtggttttt gcggccgacg accggatcgc
      361 gattcgcgcg ttcggtgatg ttattgctga ggggcacgct gggattttcg aggaagaggg
      421 tgagtccact ccagaccccc gtcatgtagt cgatggcttt ggcgagcgca ttgcccggca
      481 tggtcgtgac ctcgaccgcc cacttgccga tggcctcgac gatcggtttg gagcgctcgg
      541 tgcgcagtgc cagacgtgct tggcgcgcct ctggagagtc tccaatagca agtcgctcga
      601 tctcgaagag ctcattgatg tgcgagagca cggtcgcggt ctgggcggga aagaacttct
      661 ccacttcctt gaattcacgt ctcacgtgag cccagcaatt ggcgatgcgg agcccgggca
      721 gcttcttggc ggcagctgtg taccccttgt agccgtcgca caggacgatg ccgcggaatc
      781 ctttgaacag ctcgagcacg acggtgtgag cccgtgagct ttcaatcttg tagtagacgg
      841 cggttggcgc ggcgagcgtc cagacttgcc aagtcttacc ctcctcgaca tcggacttgc
      901 ccatcatgcg ccacgtagtt tcgtctgcac cgactacggc gtgctccagt acgaattggc
      961 gaatacgctc gtaggcaggg tggaccagtg tggcgagctt ctcgatttgg tcccacaggg
     1021 tttgggtgtc tacttccaat ccttccgctc gcatctggcg tacctgacgc gccaagggca
     1081 tgtgatacag gtacttctgg acagcaactt cgatcgcgaa gtcgatcgag tagcgggctc
     1141 tcggaaagag cttgcgaggc ccgggcgccg tctcgatccc acagttgttc gggcagcggt
     1201 acttctgctg gacgtgctcg acgttcacaa aaacgcggcg gatgacgtga atctcagtgc
     1261 tgcgatccgc ttggtctcca aacttctcga gtcggccgcc gcagacctta caagcgcaat
     1321 ctgcatccac gagctcatgg cgtttcgtga tgcgaggaag cttcgattgc tcccttcgac
     1381 catggccccg ctgtggcttc gggtctgcct gcgcttcatc gttcggactt tggtcgtcgc
     1441 tagcggagct ctcagagccg tcgtctcgag tgcgctcgct gctcggtccg aagagctgct
     1501 tttgtgcgcg cgcgagctgc acctcgagca gttgcaaccg gagctggaca tcttcgccgc
     1561 taccgcccat cagctcggtg atctgcttgg tcagcttgac gacttcgctg accagccgtt
     1621 gattctcgcg ctcgagaagg agagctgcgt tccgcagaac ttccgggtcc ttctctttgc
     1681 tgaggtcgag cgtcacaccc gtggaataga aaatcaggcg cggtatgtcg aggcttttcg
     1741 aaggcctagt ctcacatcga tcgcacctct gcgcaccagg tgcgatcgat gttggtggtc
     1801 gtgacgaagc gcgtgacgcc ggatgcgtca gcggaacacg acgcggcgat cggcgggcga
     1861 cacgatgctc gggctcaacg cgacgcgacc cacgagctcc gcgccctcga ggaacagcgc
     1921 tagctcactc tccgtgaatt ccacgcgtcc ttcccagggt gccgcgaagt gccctctgtc
     1981 gagtcgtttc gcgagaagac ataggcccgt cccgtcgaag tagagcacct tcgcgcgcgt
     2041 ccgactgcgg gccacgaaca ggaacaagtc cccgcgaagc agatcgtgac ccatggcggc
     2101 ggttaccaaa acggcgagtg aatcgtactg cttgcgcata tcgcaatggg agccgtacgc
     2161 gaacactcgg cgtctgcgac cgctgccgat catgcgagcc tcgcaagcaa ctccgcgagc
     2221 ccgtccacgg tcaggccttc gatgcgcagc tggccgggcc catgtacaag tagcgtggtc
     2281 accgtgtgcg gcggcgtcgc gctctcaacg accctcactt gacgaaagcc cctcgtcgag
     2341 cgcctcctcg acgtgtccgc ccgcgccgcc gcgtccccgg cagccagcca cttacgcacg
     2401 gtgaccagcg ctatgcctgt gtctgtggag gcctgtgcca ggctgccgcc tctctgcagt
     2461 agcgaacggg agtgcgccac caccattgcc cgaacttgct ccgggtagcc ccgtcgaccg
     2521 cgcgcctcgc cagattcccg cgctgcccga accctatctt gaatttccgt actattcatt
     2581 gtcgcctcct gcgcgcgtcc gcgcgccgaa gcgatgctcg ctggcggccc ccctcaggtc
     2641 acgacggggt tgggcgagta cttacggttg tccacggctc gctacgccgc ctcccgcgcc
     2701 cgaagtcaaa agccaagccc aagggacgcc gtgacctact cagctgcgac ctgccgagaa
     2761 tcctcgtcga cattcgcgac ccgaagcttg agcaagtcgg taagcacatc ggctgggacg
     2821 agtcgccgca gttgatgtac cgccgcggcg gcttctcaat ac
//
LOCUS       DASTKU010000874        11015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1063693, whole genome shotgun sequence.
ACCESSION   DASTKU010000874 DASTKU010000000
VERSION     DASTKU010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11015
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063693"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /gene="mrdA"
                     /locus_tag="VFQ61_30755"
     CDS             <1..1431
                     /gene="mrdA"
                     /locus_tag="VFQ61_30755"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET9958925.1"
                     /translation="YYPYGELGAHLVGYMREVDAETLARLEERGYRGGDRIGAIGIER
                     RWESYLRGQRGMRKLLIGQRSKLGRESADDLETRYLEEPRRIEPIPGRDVSLTIDIDM
                     LASVEKALRGQLAGAVVVVDVRTGRILAALSKPSFDPNVVSGGSGIAAAADAFRRLNS
                     DPLKPTLDKTISAAYPPGSTYKPFSALAGLADGLIDPDAEVECRGGYEYGKRFFRCTG
                     VHRHVNLHDALVQSCNSYFYDLGARISIDRLAAIGLDYGFGIKTGIGVNPEARGRMPT
                     RAWYTRRYKEAFRGGFTLLSSIGQGATTVTVLQLALAYAALANGGTLYQPQVVRSIET
                     SDGTVVQEFPPRVRRMVSVDAEHLSIVKNGLEGVVTDEKGTAHKERAEGVDMAGKTGT
                     AQVTHVTPRGVDPGKIWYFNRDHAWFSGYAPSRSPEIAIVVLIEHGGGGGKNAVPVAM
                     RVVRDWQKLKEKRLSARTDPDARGAL"
     gene            1446..2549
                     /gene="rodA"
                     /locus_tag="VFQ61_30760"
     CDS             1446..2549
                     /gene="rodA"
                     /locus_tag="VFQ61_30760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HET9958926.1"
                     /translation="MSFRGGPVIDMPLMLAAVAIVTLGLVNLYSATSVYVENAARSRL
                     ADNYVSQIYWVVVGALLAILTAAIDYRNYERLATFLYVGGMMSLGFVFILGSDIRGSS
                     RWIEIGGFAFQPSEFMKIVVILVIARFVHNDPKTEPRTLVDLFPALALTALPCAAVMA
                     QPDLGTSIIYAMISLSMLAMNRVRTRSLLTLLVAAGTAIPLAWTYLLRDYQKARITSF
                     LNPEADKTGTGWHALQSQTAIGNGQLFGEGFMQGTQNQFGMLPDQFSDFPFAVFAEDW
                     GFVGATFLICLYAFLCIWAIHIASQAKDRFGAAVAVGVGAMVFWHAICNIGMAIGVLP
                     VVGITLPLFSYGGSSVVTMMIGLGLLMNVSMRR"
     gene            2686..3252
                     /locus_tag="VFQ61_30765"
     CDS             2686..3252
                     /locus_tag="VFQ61_30765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9958927.1"
                     /translation="MKPSFSELLLAELASLLRVARRLTRSEVDAEDLVQATLLRALER
                     KGDLRDGERMRAWLLQVQRSVLINGNRGLSRKLEVVRGSLAQAEEPSADLEAEIMDRR
                     FTDELQRALERLAPELRDAILLREVEGLAYEEIARIQGCPTGTVRSRLARARLALIEW
                     LSQNTEASKWQAAPEIGSRVSRRGTTVK"
     gene            3192..3932
                     /locus_tag="VFQ61_30770"
     CDS             3192..3932
                     /locus_tag="VFQ61_30770"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9958928.1"
                     /translation="MAGCTRDWFESVSTWHDGEVTPEDAERIEAHVRSCPECRLDAMR
                     LGRIASALSESVEHEVPDRVSASARALMKRHRRRRWPWAGGAIVAAAALVLVFRFGRA
                     SLPEAVAEEIVHRHVAGFARAPQQRFEASNAVTVSDWLSERMGYPVKVRAPANTELVG
                     ARVCSLCGSGTAAVMLRRGGTPVTIFIPPPGSEAARAAERLARKGVACTPGPDGNSIC
                     ACRGAQPMLAVAASAPEQVSAALMAALP"
     gene            3941..5023
                     /locus_tag="VFQ61_30775"
     CDS             3941..5023
                     /locus_tag="VFQ61_30775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein/redoxin"
                     /protein_id="HET9958929.1"
                     /translation="MPIPALLTLYLAGIATFASPCVLPLLPVYLAALGSADPERRRPL
                     WHNAAGFTLGLSGVFVALGLTATALASALSSHRRVMMLGVGIIMLVLGGKLLGVLRLP
                     GLDREARPVLSRLPSAHGFFGSLLFGVGFALGWTPCVGPVLAAALGYAASHSASLGSA
                     ALQLGVYALGLSTPLILASLAAQRVLELSRRLRGVTPFVQRGLGALLVLVGVLVATDR
                     ISAVVPSVKTEDETCVSQQGNACLVPELATGAASDGEPQRLPAGKPRLLEFVSAHCPV
                     CARMAPLVAELERACTQGDGTMLRLAVESPEGRALAQRYQVHAVPTFLQVDSDGNEVE
                     RALGEQSRTQIQNALESVRGSACPSL"
     gene            complement(5101..8415)
                     /locus_tag="VFQ61_30780"
     CDS             complement(5101..8415)
                     /locus_tag="VFQ61_30780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HET9958930.1"
                     /translation="MSSLADALSTLSDRGLRRLLGARTFLRGLDYEKRKVVDEVHLNE
                     GSAHGRVKGSDPDPYEVTIRLGGEGITSHCTCPAFQKTGQHCKHVAALLITVRNKARQ
                     QMPRREAPPQVMAPPPPPPIQQQQPQGDNGKGSRRRDRRRGRVPTPPVPAVQGHFPPP
                     IPELSARQTGLGAWLSPEGGMRQVKLEFRVHVRQGGLTATVLDVDNRAPLLPSAALAW
                     QATQPSPDRNALRVLARFESGNPRHPAVDIRGEDAAELLPLLRDRRVLLEPALMQLRF
                     LEEPLRPRFDLEMVSDTIVAKVTFERPSDGRRFSLTSGGWFEGAPGYHIDTTEGIARP
                     LDHRVSPAALRRLLRSPTIAEPASELISLITYGLPKVALEVGAELPELSQVVDVIDLT
                     PEFRMRATGSLTQVEASLRAQYGEREVEVRADGISPPIMIMPPEEGQKRMRCIRCDIA
                     AQQLAVQELLELGLKADESGEFFVAEGETAIKFWSEGVGSLPEDWELYIPEELLGTQV
                     RNAPLTMNARVSSGVDWLSVKISYESDGVGVDREELERCLREGKKFVRLSDNSYAPLD
                     PEKVQAMIDREIELIQAGGKAGKIPLSQAGRVQELLQQANSAHVAASAKQLFQKLASI
                     DEIKAAKKPKALKATLRPYQEQGLSWLSFVHEIGSGGVLADDMGLGKTIQTIALLLNI
                     KAEKKTQPLRALIVAPTSVVSNWVREIQRFAPTLTTALWHGAGRKEQMEEMETANVII
                     TSYALLRRDIDLLKKLRLDYAILDEAQNIKNPLSATAQAAKELTADRRLALTGTPIEN
                     RLSEIWSIFEFVSPGLLGPLPKFEEKYARPIDNGDSKAASRLRAVIHPFILRRTKIEV
                     AKDLPPKLEVDKLVDLAPDQKAIYLQVLREVRATVMGEVERVGMAKSQLHILAGLTKL
                     RQAACDPRLLGLPREFSHEDSGKLMALRELIDEAESGGHKVLVFSQFVSMLKLVREAL
                     EEDKIKYEYLDGSTIDRAERVDRFQEDPTVPVFLISLKAGGSGLNLTAADTVIHFDPW
                     WNPAVEDQASDRAHRIGQKKVVTVYRLVAAGTIEEKILQLKQKKKDLVSSVLSEDAGG
                     AKTLSRDDLDDLFKVD"
     gene            complement(8461..10578)
                     /locus_tag="VFQ61_30785"
     CDS             complement(8461..10578)
                     /locus_tag="VFQ61_30785"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HET9958931.1"
                     /translation="MTELALILGIQVAAFAYAWVAGQALASRAVSSVPLRRLGAALER
                     ATGHFSGKQLRVLGLLALAAAVLLFGLYASHSGGTQVGRLPAAVTAALSVGAGASLSW
                     LATRMALRVGLRANIQVALSTRTGLDAALSLAMRSGAIISLFSEGLSGIGLIGLFGAV
                     FALLGGTGLPLDASLIRASDVVSTLSGFPLGAALTALVCQSSASTYRAAVGIGSEVAS
                     DKRFALTPNDARNPALVGELAGGHLGETATRAALSFMTAATAQVATLSLGLAVFAKSP
                     ERLTTSLLLLPFVVRAFFVLATVFSSVLVRTEEMTNPSLAILRGYLSCLAIGLSGVVG
                     SCLWLARGALAVFIGAGVLGVAAAGLVALPLWLRLLRPAAWLRDAADAARSGSTPGAV
                     LSSAFFSLGSALQASLLPTLAVAAASVVSFRIGQASGLPYGGVLTTLVTWAAMMGCAP
                     FAAAVSQVGTLAVGTRGVVALGQLDPEAQFRSVRLEETGVVAGSARAQLIASGAGACM
                     LAVLAIPALSRAPALDAGTTFSPLPVWCGALGLIFVLSYAGSCVRAAVSAAREVSSEV
                     ERQLKVLGRDTNAAFSPSYKACVDLSTRLASRKLTPYALLAVGAPVLLALGLRALRAP
                     VDDPTLAAFSSFVRATGLLGFALALALDAARTTLSLASPRSAPDPNSASSAAIFGHAA
                     APAAHTLMVGTGALALAIAPFLN"
BASE COUNT         1932 a         3518 c         3598 g         1967 t
ORIGIN      
        1 tactatccct acggcgagct cggggctcac ctcgtggggt acatgcgcga ggtcgacgcg
       61 gagacgctgg cccgcttgga agagcgaggc taccgcggag gcgatcgcat cggcgcgatc
      121 ggcatcgagc gacgctggga aagttacctg cgcggtcagc gcggcatgcg caagctgctg
      181 attgggcaac gctcgaaact cggacgcgag agcgcggacg atctcgagac ccgttacctg
      241 gaggagccgc gccggatcga accgatcccc gggcgcgatg tttccctcac catcgacatc
      301 gatatgctcg cgtcggtgga gaaggcgctg cgcggccagc tcgcgggcgc ggtcgtggtc
      361 gtcgacgtgc gcacggggcg aatcttggcc gcgctctcga agccgagctt cgatcccaac
      421 gtggtctcgg gcggcagcgg tatcgcggcc gcggccgacg catttcggcg gctcaacagc
      481 gatccgctga agcccacgct cgacaagacg atttcggccg cctacccgcc gggttcgacg
      541 tacaagccgt tcagtgcctt ggccgggctc gcggatgggc tgatcgatcc cgacgcggag
      601 gtggagtgcc gaggcggata cgagtacggc aagcgcttct tccggtgcac cggagtgcat
      661 cggcacgtca acttgcatga cgcactcgtt cagtcgtgca acagctactt ctacgatctc
      721 ggcgcgcgca tcagcatcga tcgcctggct gcgatcgggc tcgactacgg cttcggcatc
      781 aagaccggca tcggcgtgaa cccggaggcg cgcgggcgca tgcctacccg cgcctggtac
      841 acgcgccgct acaaggaagc gttccgcggt ggtttcacgc tgctgtcctc gattgggcag
      901 ggcgccacga ccgtcaccgt tctgcagctt gcgctggcct acgctgcgct tgcgaacggc
      961 ggcactctct atcaaccgca ggtggtgcgc agcatcgaaa ccagcgacgg caccgtggtt
     1021 caagagttcc caccccgcgt ccgacgcatg gtcagcgtgg acgctgagca cctgtcaatc
     1081 gtgaagaacg gcctcgaagg cgtggtcacc gacgagaagg gtaccgcaca caaggagcga
     1141 gccgagggtg tcgacatggc cggaaagacc ggcactgcgc aggtgacgca cgtgaccccg
     1201 cggggtgtcg atccgggaaa gatctggtac ttcaaccgag accacgcctg gttctcgggc
     1261 tatgcgccat cgcgctcccc ggagatcgcg atcgtcgttc tgatcgagca tggcggcggt
     1321 ggcggcaaga acgcggtgcc ggtggcgatg cgcgtggttc gggattggca gaagctcaaa
     1381 gaaaagaggc tctctgcccg tacggatcct gacgcccggg gggcgctctg atgcgcggag
     1441 atcgcatgtc tttccgcggc ggccccgtga tcgacatgcc cttgatgttg gccgcggtgg
     1501 cgatcgtcac gctcgggctc gtcaacctgt acagcgcgac cagcgtttac gtcgagaacg
     1561 cggcgcgttc gcggctcgcc gataactacg tctcacaaat ctactgggtc gtggtggggg
     1621 cgttgctcgc gatcttgacc gcggccatcg actaccggaa ctacgagcgg ctcgccacgt
     1681 tcttgtacgt gggcggcatg atgtcgctcg ggttcgtgtt catcttgggc tcggacatcc
     1741 gcggctcttc acgctggatc gaaatcggcg gtttcgcgtt tcagcccagc gagttcatga
     1801 agatcgtggt gatcttggtg atcgcgcgct tcgtgcacaa cgatccgaag acggagccgc
     1861 gcacgctcgt ggatcttttc ccggcgctgg ctctgaccgc gttaccctgc gcggccgtga
     1921 tggcgcagcc cgatctcggg acgtcgatca tttacgcgat gatctcgctg tcgatgttgg
     1981 cgatgaatcg cgtgcgcacg cgaagcctac tcacgctgct cgtggccgcc ggtacggcca
     2041 ttccactcgc ttggacctac ctgctgcgcg actaccagaa ggcacggatt acgagcttcc
     2101 tcaatcccga agccgacaag accggcacgg gttggcacgc cctgcaatcc cagaccgcga
     2161 tcggcaacgg gcaactgttc ggggaaggct tcatgcaggg gacgcagaac cagttcggca
     2221 tgctgccgga ccagttctcc gactttcctt tcgcggtgtt cgcggaagat tggggattcg
     2281 tcggggcgac cttcctgatc tgcctctacg cattcttgtg catttgggct atccacatcg
     2341 cttcccaagc gaaggatcgc tttggggcgg cggtggccgt tggcgttggg gcgatggtct
     2401 tctggcatgc catctgtaat atcgggatgg cgatcggcgt gttaccagtg gtgggcatca
     2461 cgttaccgct gttctcctac gggggctcca gcgtcgtgac gatgatgatc gggctcggac
     2521 tgctcatgaa cgtctcgatg cgacgctgag ccgctgcgcg cgggcgggcg cccggcaatt
     2581 ggctcgaaaa ccgtgttctt gcgggtgctc gtagaaacgg ggaacttttt ggagggctgc
     2641 tagtgtcttc aaagagctgg cgccggacgg cgccggcccg cgacgatgaa accttccttt
     2701 tcggagctgc tgctggctga gctcgcgagc ttgctgcggg ttgcgcgtcg gctcacccgt
     2761 tcggaggtgg acgccgagga tctggtgcag gcaacgctgc ttcgagcact ggagaggaag
     2821 ggcgacctgc gggacggaga gcgcatgcgc gcctggctcc tccaggtaca gcgtagcgtg
     2881 ctgatcaatg gaaaccgtgg cctgtctcgg aagctcgagg tcgtgcgcgg cagcttggcc
     2941 caggcggaag agccgagcgc ggatttggaa gccgagatca tggatcgacg cttcacggac
     3001 gagttgcaac gagctttgga gcggctcgcg ccagagctca gagacgcgat cctgctgcgc
     3061 gaagtcgagg gcctggctta cgaggagatt gcacgcattc aggggtgccc gaccgggacg
     3121 gtgcgctccc ggctcgctcg cgcgcggttg gctttgattg aatggttatc ccaaaacacc
     3181 gaggcttcga aatggcaggc tgcacccgag attggttcga gagtgtctcg acgtggcacg
     3241 acggtgaagt gaccccggaa gacgccgagc gcatcgaagc gcacgtgcgt tcgtgccctg
     3301 agtgccggtt ggacgcgatg cgactagggc gcatcgcgag tgcgctctcc gagtcggtcg
     3361 agcacgaagt gccggaccgg gtgagcgctt ctgctcgcgc gttgatgaag cgacatcggc
     3421 gtcgtcgctg gccgtgggcc ggcggtgcga tcgttgcagc cgctgcgctc gtgctggtct
     3481 ttcggttcgg tcgggcgagc ctgcccgaag ccgtggcgga ggagatcgtt catcgccacg
     3541 ttgcaggttt tgcgcgcgct cctcaacaac gcttcgaggc atccaatgcc gtaacggtgt
     3601 cggactggct ttccgagcgc atgggctatc cggtgaaggt acgcgcgccc gcgaataccg
     3661 agctggtagg cgcgcgcgtt tgtagcctat gtggcagcgg tacggcggcc gtgatgctgc
     3721 gacgaggtgg aacgccggtg acgatcttca ttcctccgcc cggatccgag gcggctcgag
     3781 ccgccgaacg tctcgcgagg aaaggcgtgg cgtgtacgcc ggggcccgac gggaactcga
     3841 tctgcgcgtg ccgcggagcg cagcccatgc tggcggtggc cgccagcgca ccggagcaag
     3901 tttctgccgc tctgatggcg gcgttgcctt gatgagcacc atgcctatcc ctgcgctgtt
     3961 gaccttgtat ttggctggaa ttgcgacgtt tgcttcacca tgcgtgctgc cgttgttgcc
     4021 ggtctatttg gcggccctag gcagcgccga tccggagcga cggcgtccgc tttggcacaa
     4081 cgcggccggc ttcacgttgg gtctgtcggg cgtgttcgtc gcgctcgggt tgactgcgac
     4141 ggcgttggcg tccgcgctgt cctcacatcg gcgagtgatg atgctcggcg tcggcatcat
     4201 catgctggtg ttgggaggca aactgctcgg tgtgttgcgc ttgccgggct tggatcgcga
     4261 ggcacgaccg gtgctatctc gcttgccgag cgctcacggt ttcttcggta gcctattgtt
     4321 cggcgtgggc tttgctctcg ggtggacgcc gtgcgttggc ccggtgctgg ccgccgcgtt
     4381 gggctacgca gcgagccatt cggcgtcgct cggaagtgca gcgctacaac tcggagttta
     4441 cgcactcggc ctctcgactc cgctgatttt ggcgagcctc gcggctcagc gcgtgctcga
     4501 gctgtctcgg cgtctacgcg gcgtcactcc gttcgttcaa cgcgggttag gcgcgctgct
     4561 cgttctggtc ggcgtgctgg ttgcgactga caggatttca gccgtggtcc cgtccgtgaa
     4621 gaccgaggac gaaacgtgcg taagccagca gggcaatgcc tgcctggttc cggagctcgc
     4681 gacgggagca gcgtcggacg gagagcctca gcgactcccg gcgggcaagc cgcggctcct
     4741 ggaattcgtg agtgctcatt gtccggtttg cgcgcgcatg gcgccgctgg tggcagagct
     4801 cgagcgcgct tgcacgcaag gggacggcac gatgttgcgg cttgccgtgg agagcccaga
     4861 ggggcgcgcg ctggcgcagc gctaccaggt gcacgccgtt ccgacgttct tgcaagtcga
     4921 ctccgacggc aatgaggtcg agcgcgcgct cggcgagcag agccgtactc aaattcagaa
     4981 tgccttggag agtgtacgcg gttccgcgtg tccgtcgctc tgacgtccgc gcccaccggg
     5041 gctggtcagc gcgacgcgca cggcgctccg acccttgccg tggtgcggcg cggcgggtgc
     5101 tcagtcgacc ttgaacaagt cgtccaagtc gtcgcgcgag agcgtcttcg cgccgcccgc
     5161 gtcttcgctc agcaccgacg agacgagatc cttcttcttc tgcttcagtt gcaggatctt
     5221 ctcttcgatc gtgccggcgg cgaccaatcg atacaccgta acgaccttct tctgaccaat
     5281 gcggtgagcc cgatctgaag cttgatcttc gacggcgggg ttccaccagg gatcgaagtg
     5341 aatgacggtg tccgccgcgg tgaggttcaa gcccgaaccg cctgccttca ggctgatcag
     5401 gaacaccggt acggtaggat cctcctggaa ccgatcgaca cgttcggcgc ggtcaatcgt
     5461 cgaaccgtcc aggtactcgt acttgatctt gtcctcttcc agcgcttccc gcaccagctt
     5521 gagcatcgac acgaactggc tgaacacgag caccttgtgc ccgccagatt cggcctcgtc
     5581 gatcagctct cgaagagcca tcagcttgcc gctgtcctcg tggctaaact cgcgcggcaa
     5641 tccgagcaac cgcggatcac aggcggcttg tcgcagcttg gtgagcccag ccaagatgtg
     5701 gagctggctc ttcgccatac ctacgcgctc cacctcgccc atgacggtcg cccgcacttc
     5761 gcgcaatacc tgcaggtaaa ttgccttctg gtcgggggcg agatcgacga gcttgtcgac
     5821 ctcgagcttg gggggcaagt ctttggccac ctcgatcttg gtacgacgca agatgaaggg
     5881 gtgaatgacg gcgcggagcc gtgacgccgc tttggagtcc ccgttgtcga tggggcgggc
     5941 gtacttctcc tcgaatttgg gcaggggccc gagcaaaccc gggctcacga actcgaaaat
     6001 cgaccagatc tcgctcaagc ggttctcgat cggcgtgccc gtcagcgcga ggcgacgatc
     6061 agcggtcagc tccttggcgg cctgggccgt ggcgctcaga ggattcttga tgttctgcgc
     6121 ctcgtcgaga atcgcgtaat ctagccgcag cttcttcaag agatcgatgt cgcgacggag
     6181 cagcgcgtag ctcgtgatga tcacgttcgc ggtctccatc tcctccatct gttctttacg
     6241 gccggccccg tgccacaagg cagtggtgag ggtaggggcg aagcgttgga tctcgcgcac
     6301 ccagttgctg accacgctgg tcggcgccac gatcaaggct cgcagtggct gagtcttctt
     6361 ttcggccttg atgttcaaga gcagcgcgat ggtctggatg gtcttaccga gacccatgtc
     6421 gtcggcgagc acgccgccgg acccgatctc gtgcacgaac gacagccagg acaagccctg
     6481 ctcctggtag gggcgcaggg ttgccttcag agccttgggt ttcttcgcgg ctttgatctc
     6541 atcgatcgag gcgagcttct gaaagagctg cttggcgctg gcggcgacgt gcgcgctgtt
     6601 ggcctgctgc agcagctcct gaactcggcc cgcttgtgag agtgggatct ttcccgcttt
     6661 tccgccggct tgaatcagct cgatctcgcg atcgatcatg gcctggacct tctcggggtc
     6721 cagtggagcg taggagttgt ccgacaggcg tacgaacttc ttgccctcgc gtaagcagcg
     6781 ctcgagctcc tcgcgatcga cccccactcc gtcactctcg tagctgattt tgacgctcaa
     6841 ccagtccacg ccgctcgaga cgcgcgcgtt catcgtgagc ggtgcgttgc gaacctgagt
     6901 gccgagtagc tcttccggaa tgtagagctc ccagtcttcg ggtagcgagc caacgccctc
     6961 ggaccagaat ttgatcgcgg tctcgccctc ggccacgaag aactcgccgc tctcgtcagc
     7021 cttcaagccg agctcgagca gctcttgcac tgcgagctgc tgcgcggcga tgtcgcaacg
     7081 gatacagcgc atgcgcttct gcccctcttc gggcggcatg atcatgatgg gcggtgagat
     7141 cccgtcggcg cgtacctcga cttcacgttc gccgtactgc gcgcgcaacg aagcctccac
     7201 ttgcgtgaga gacccggtgg cgcgcatgcg aaactccgga gtcagatcga ttacatcgac
     7261 cacttgcgag agctccggga gctccgcgcc cacttccagc gcgaccttgg gcaagccata
     7321 ggtaatcagg gaaatcagct ccgaggcagg ctccgcgatg gtaggcgagc gcagcaaacg
     7381 cctcaacgcc gcgggcgaca cccgatgatc caatggtcgc gcgatgccct cggtcgtatc
     7441 gatgtgatag ccgggcgcgc cctcgaacca gccgccgctc gtcagcgaga aacgccgacc
     7501 atccgaagga cgctcgaacg tcaccttggc cacgatggta tcgctcacca tctcgagatc
     7561 gaagcgcggc cgcaacggct cctcgaggaa gcgtaactgc atcagcgctg gttccaggag
     7621 cacgcgccga tcgcgcagca atggcagcag ctcggccgcg tcctctccgc gaatgtccac
     7681 ggctggatgc cgcgggttcc cgctctcgaa gcgtgccagt acgcgcaagg cgttgcgatc
     7741 gggcgacggc tgcgtggctt gccaggccag ggcggcgctc ggcaacaacg gcgcgcggtt
     7801 gtccacgtcg agcacggtgg ccgtcaatcc accctgccga acgtggacgc ggaactcgag
     7861 cttgacctga cgcatgcccc cttctggcga cagccaagca cccaacccgg tctgtcgcgc
     7921 gctgagctcc gggattggcg gcggaaagtg cccctgcact gcgggaactg gcggcgtggg
     7981 gacgcggccg cgacgacgat ccctacgtcg gctgcccttc ccattatcac cctgcggttg
     8041 ctgctgttgg atgggaggag gggggggcgg cgccatgacc tgcggtggcg cctcgcggcg
     8101 gggcatctgc tgtcgcgcct tgttgcgcac cgtgatcaac aacgccgcga cgtgcttgca
     8161 gtgttgcccg gtcttttgga acgccgggca cgtgcaatgc gaggtgatgc cttcgcctcc
     8221 caagcggatg gtgacttcgt acgggtcggg atcggaaccc ttgacgcgac cgtgcgcgct
     8281 accctcgttg aggtgcacct cgtcgacgac cttccgcttt tcgtaatcga gaccacgcag
     8341 aaaggtgcgt gctccgagca aacgacgaag accgcgatcg gagagtgtgg agagggcgtc
     8401 ggctagtgag gacaaggtag cttcgttttt cggttcaagg gcggcccggt cggtgccgcg
     8461 ttagttcagg aacggtgcga tggcgagcgc caaggctccg gttcccacca tcaacgtatg
     8521 ggcggcggga gccgccgcgt ggccaaaaat cgccgctgag ctcgcgctat tgggatcggg
     8581 agcactgcgc ggcgaggcga gagacaacgt ggttcgagct gcatcgagcg ccagcgccag
     8641 cgcgaatccc aacaagcccg tggctcgtac gaaactgctg aaagcggcta gcgttggatc
     8701 gtccaccgga gcgcgcaaag cccgaagccc cagcgcgagt aacacggggg cgcccaccgc
     8761 caacaaggca taaggcgtca gcttgcggga cgcaaggcgc gtggatagat cgacgcaagc
     8821 cttgtagctc ggcgagaatg cggcattcgt atcacggccg agcaccttga gttggcgctc
     8881 gacctcactg ctcacttcgc gagctgcgct aaccgcggca cgcacgcagg agcccgcata
     8941 actcagcacg aagatcaggc ccagcgctcc gcaccacacc ggcagcgggc tgaacgtcgt
     9001 tccggcgtcg agcgcggggg ctcgagacag ggcagggatg gctagcaccg ccaacatgca
     9061 agcgcccgct ccgctggcga tgagctgagc acgcgcgctg ccggcaacga cccccgtctc
     9121 ctccaagcgc acgcttcgaa actgcgcttc gggatccaac tggcccagcg ccaccacgcc
     9181 gcgcgtcccg accgccaacg tacccacctg actgacggcc gcggcaaacg gtgcgcagcc
     9241 catcatcgcg gcccaggtga cgagagtggt caacactccc ccgtaaggca acccgcttgc
     9301 ttgcccgatc cggaaggaca ccacgctcgc ggcggcgacc gccaaagttg gaagcaaact
     9361 agcctgtagc gcgctcccca ggctgaaaaa cgcagaactc aacaccgccc ccggggtact
     9421 tcccgaacga gcggcgtccg ccgcgtctcg cagccacgct gcgggcctga gcaaacgcag
     9481 ccagagcggc agcgccacga gccccgctgc ggccacacct agcacgcccg cgccgatgaa
     9541 caccgcgagc gccccgcgcg cgagccacaa acaagagccg actacgccag agagaccgat
     9601 cgcgaggcaa ctcagataac cccgcaggat ggcgaggctc ggattggtca tctcctcagt
     9661 gcgtaccaac acggaactga acacggtggc cagcacgaag aacgcgcgca ccacgaacgg
     9721 taacaacaac agagaagtcg taagcctctc gggcgatttg gcgaacaccg cgagacccag
     9781 cgataaggtc gcgacctgtg cggtggcggc cgtcatgaac gacagtgcgg cacgcgtcgc
     9841 tgtctccccc aagtgaccgc ccgcaagctc accgacgaga gctgggtttc gtgcatcgtt
     9901 gggggtcaaa gcaaagcgtt tgtcactcgc aacttcgctc ccgatcccga ccgcagcccg
     9961 atacgtgctc gccgagctct gacaaaccag cgccgtgagc gcggccccca gcggaaatcc
    10021 agaaagggtc gaaaccacgt ccgacgcgcg gatcaggctt gcatccagcg gcaggccagt
    10081 gccgccaagc agcgcgaata cggctccgaa tagaccgatt agcccaatcc cgctcaagcc
    10141 ctcgctgaac aagctgatga tcgcgcccga acgcatggcg agcgaaagcg cggcgtccaa
    10201 gcccgtgcgg gtggacagcg caacctggat gttcgctcga aggcctacgc gaagcgccat
    10261 gcgggtcgcc aaccacgaaa gcgacgcccc cgcgccaacg ctcagcgccg cggttaccgc
    10321 cgcgggcagt cgcccgactt gggtcccgcc gctgtgactt gcgtacagcc cgaacagcag
    10381 cacggccgcc gctagcgcca gcagccccaa gacccgcagt tgcttaccag agaaatgtcc
    10441 agtcgctcgc tccaaagcgg cgcccaaacg acgcagcggt accgagctca cagctctaga
    10501 agccagagct tgcccggcca cccatgcgta agcgaaggcg gctacttgga tcccgaggat
    10561 cagcgcgagt tccgtcacgg cagacagcag ggcgcggctt ggacgcccga atcaggcgac
    10621 gagagaaacc aggacgctgc cggaattctt ctcgtcggcc aaaccactcc agggtttacg
    10681 actggccata taccgggccg ggcaacctcg ggcccagcca ccgacgccag agcgctcccc
    10741 caaggagcga tcagccttga tagacggcga ggggcttagc atggacccgc agcggggagc
    10801 aagcgaccct tcgcgcaaaa gagtgtggtg gcacgccccc gacctcaacc acgcgcagcg
    10861 acgagccgac cttcagcctc gactcgtcgc cacgttcagc cgcgcttttt ggactgcatc
    10921 accatcagat gataccgacc aaactcgctc tgccccagag tgaacaacac ctcgatcagc
    10981 agacggtagg gggtgctgcg atcggcgatg atgat
//
LOCUS       DASTKU010000875        13607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_617148, whole genome shotgun sequence.
ACCESSION   DASTKU010000875 DASTKU010000000
VERSION     DASTKU010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13607
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617148"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFQ61_30790"
     CDS             <1..506
                     /locus_tag="VFQ61_30790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958932.1"
                     /translation="PDGKRAAQACTGHLTATGEIEDLSQSALVFYDVSLSGLKETERL
                     DVAQLGVEGLQNDVVFANAEYALIKSQTPYGGTSHNRWIALELSSKKVSTLLEARPDE
                     NGEGKGLTYGAMSCSPGCSDVCLLADSDRGVLQRVQIEAGRSPKLLTPVTVEEQVGLP
                     PRGIASR"
     gene            576..1478
                     /locus_tag="VFQ61_30795"
     CDS             576..1478
                     /locus_tag="VFQ61_30795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958933.1"
                     /translation="MRSWAWLFGVTPVLAAFFPGHLELGSPNFANAAYATHETSSPSP
                     RDVTRIASGSLISDELLFDLCPERLVAVSYVADQAHVTMLAGRVASKLPRITANAEQI
                     LAVEPDLAVFSDFSSMQVEALVRSAGHSVARVRAPHDFDELLASIQLLGRQVGATNAA
                     ANLSRDLTTRLAALARARPRKPFRTLLVQSGYAYAEGTLQHDCLKRIGLDNALEPWHV
                     HGTPALHQELFMLARPELVFVASEKPEVHSESGSNLVERGILPEAAARVPLIFSVPET
                     WMGSLTHYGLRACEAYARVAGRLP"
     gene            1475..2467
                     /locus_tag="VFQ61_30800"
     CDS             1475..2467
                     /locus_tag="VFQ61_30800"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET9958934.1"
                     /translation="MNPRYLYAALTLLLIVSLSASIALGAVPVPLLAALRPGPDWDWV
                     RRILWSVRLPRALCGCIVGAALSSSGLVLQSVFRNPLAEPSVLGVSGCAALAAQLALF
                     SGLGQLASYSVPLAAVIGAAVGISLLLFLVRGLHHVPEPVLISGVALGQLALAVSALL
                     LSLALRDYSIARRLLTWLLGGLDGRTWLHVLWGVGPLVAAILRIAARSREIDALTLGD
                     TTALAVGVDVARSRRELVLIAAVLSGIAVAIAGIVGFVGLIIPYFVKRWLQPRRGSAV
                     WACALAGGSFVVLSDLVARLLIAPEELQIGAVSALFGAPLFAWLLLRRYRELAT"
     gene            2464..3345
                     /locus_tag="VFQ61_30805"
     CDS             2464..3345
                     /locus_tag="VFQ61_30805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9958935.1"
                     /translation="MNSSVIVRPPRASSLELRSIAVWRGQRQVLHDVCLHLAPGQLWA
                     LVGPNGSGKSSLLEALLGALPCSGEILLGGHDVRRWSARERARHLTLLGRESTAQTTL
                     RVRDLVELGRLPYRRAFDALSSADHSAVDSALQRAGVLELEHRSVNDLSDGEKQRVFL
                     ARAFAQHPELILLDEATSHLDILHREQTLQGIRAFVAEGRLALMAIHDLELAVRHASH
                     VAILSEGRIVASGPALDTLTPTRLAQVFGIDAEIRQDERGNQLRIFGLAASERTQPRS
                     SNGRHPSNSSLLEVKTV"
     gene            3342..3875
                     /locus_tag="VFQ61_30810"
     CDS             3342..3875
                     /locus_tag="VFQ61_30810"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF014351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HET9958936.1"
                     /translation="MKLTFVIGGARSGKSRHAMQLARALSDNPVYLATSRIWDEDHGR
                     RIERHRKDRGPEWRTVEEPKYLSQVGVQDAVVVIDCVTLWLTNFFIDVGHDLDRCLEQ
                     AWAEFSKLDTCRNHYIVVSNELGQGLHAETEIGRKFTDLQGMINQRIAARADDVTWMV
                     AGIPVSIKHTAPSSRTE"
     gene            3900..5024
                     /locus_tag="VFQ61_30815"
     CDS             3900..5024
                     /locus_tag="VFQ61_30815"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HET9958937.1"
                     /translation="MLNLPTPDLNALAAAEQRQSRLTKPPGSLGRLEKLVLQLAAFQA
                     RPLPSARPAAALVFAADHPVTRHGVSPYPSAVTAAMLHNFREGGAAASVLARACRVPL
                     CIVDVGVAGVSELPTSSLSKGAVLRSDPFGSPSSFEVNAPATAHSGVYVWRESVADST
                     AGDLRVEDAMSEAVFEGCAAAGRRAVTRLGPLDVLIVGEIGIGNTTAAAAVCAALLGG
                     DPGELVGAGTGASGAMLETKRRVVTEAVQRVADERSPLEILRRLGGREIAAAFGAIHA
                     ALEQRSVVLVDGFVISAAALALVRANARALHGLVFSHLSGERGHARVLAELGVQPMLD
                     LGLRLGEASGALAAYPLLEQACLLHSEMATFESAQVPDKA"
     gene            5088..5912
                     /locus_tag="VFQ61_30820"
     CDS             5088..5912
                     /locus_tag="VFQ61_30820"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HET9958938.1"
                     /translation="MTAPSLPTYGLPPYLRGLRAAFVFLTRLPVGGFPYQRRDFEWAA
                     AHFPNVGFIVGAAGGLVHYLSRGLGDMAAALFALGTTAWLTGAFHEDGLADTFDALGG
                     YHNRERIFEILKDSRIGSYGSVALFVVLGLRVALLTNIAAKASSPYIAAAAVIAAQVF
                     GRVPAVWLLAITPYVSPEAQAKGSGVAGAGFPQALVATAFAALYLGGLGPLLEPPLLF
                     YPALFAALAVTGLVLRRWFLARVGGVTGDLLGATEQLSEVAVLVAWSWAFGGMAAP"
     gene            complement(5851..7626)
                     /locus_tag="VFQ61_30825"
     CDS             complement(5851..7626)
                     /locus_tag="VFQ61_30825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958939.1"
                     /translation="MQLNYSALRSYLRWIPWGRADAERAPRDGDVGQAHAPPIRVWRR
                     LGLRALGWLKVNESPTPVKWSERSRATRGTTWGLLALVVFVMAVLRRGWMCDDAFITM
                     RVVDHLVAGRGMVFNAGERVLGITNPLWALVLAGPLALTQNHYLVAVLAGVVTSALAA
                     SVLVFAVARDRALGALVLVALCFSRSFVDFSTSGLENSLTHLLLFCFYAVLFRPAVRS
                     SGKLVLLSALTVVNRIDALPLLLPGLLWTLWQERRRKGLWWRVLLGATPAIAWFVFAI
                     IYYGFPSPNTTYAKLNTDIPRAEMVRQGFSYLIDVVANDSLTAILIAAALAISIQAIR
                     TQRLAKFVLLALIANLAYVVSVGGDFMSGRFMTAATAVSAAALVHFGGELLRGPSGLP
                     LQGLALGLGALIMSLTFSPFRLEKETEKRDFPYTRIVNERSWYQDHLLLPMNVRPTAW
                     REDGLFTSGVEARQRKERVAVVGNIGLFGLGAGPDVHVLDPLALTDPLLARIPFKYDR
                     GWRTGHLGRDVPEGYVKSLEEGRNLIEDRCLAEYYDRLNQVIRGPLFTRERWEAIIAL
                     NSPGGVTVRPCPQKPKTKRPGRRLH"
     gene            8135..9004
                     /locus_tag="VFQ61_30830"
     CDS             8135..9004
                     /locus_tag="VFQ61_30830"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9958940.1"
                     /translation="MTIDSSFKRARPAPPEHTFEGASAARQSRLPDSNTQAWQPQKYE
                     QRAGFVQSGGVPVVELLAPRAGERILDLGCGTGTLTQTIAEAGAEVLGIDNSESMIQA
                     ARAKYPNLAFEIQSGEELTYVDRFDAVFSNAALHWMHRAEDVARGVYRALRPGGRFVA
                     EFGGAKNVHHVRAAVDAAARELGLPIPQPYAPWYFPKLGEYTSLLENAGFDVRFAQHF
                     ERPSPMPDQGGESGIASWLDIFARQLFQMVPAAQRARFLAAVEQHARPQLFRDGTWWI
                     DYTRLRLVATRPS"
     gene            complement(9083..10189)
                     /locus_tag="VFQ61_30835"
     CDS             complement(9083..10189)
                     /locus_tag="VFQ61_30835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958941.1"
                     /translation="MNNAVTFALAFGLLLACGSGERATQEPVSLVTGGSAGTQGGSRS
                     SQLGSGGVDRAGASGGVAEQGGSPVGSAGGAGGASEAAGGSPELDAGAGGETDGPVPG
                     PIVIPESVCAMRMRAGTPRSLGLAVTSDSRLGSVSADELSIVWTRVQAGQVTLYSAAR
                     DAVDEPFQKPTWQVIPAAPGKVALSADGLRVLFVATDGRHFEAWERRSRQDSFADTNS
                     FEFELLNDSAESGLSPAEAYGEPVLSADDRTLFYSRFGGGRTHTVFAARRFSSGAPWS
                     SGTELALPERFDASAESHPELTGVSSDLQTLFLWDPASARATAVFLEASPDAVYSSVA
                     LLGAFRGAAPSVDCKRLFYEDPTGKLGLVTADLL"
     gene            complement(10182..13376)
                     /locus_tag="VFQ61_30840"
     CDS             complement(10182..13376)
                     /locus_tag="VFQ61_30840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958942.1"
                     /translation="MSSRAWLAVSFSFAVACNGSELPPGSYEGDAPLVQVWPDACATP
                     DQGCPCSIEGQSIECGALKSENSGYATCSVGLRTCNSGLWSACAAQGTRVQAIGKQRL
                     QGLGTSQDCNNPCDPFCSRITDDSTGLTVEPGDDVEVTSSGVTLTQTETSVIGTPCTS
                     LAVTTTTPTLTVTQFSPVSPSSVQFSAALSPSGCYPGTPPVVWAIDRFDIATIDGAGL
                     LTLVSPNAGTIRVTGYSGSLSATFNLNVVLNVLDATASPSASATSAFANTVGSADTAK
                     VLYPYADTVLPLGLLSPLVQWNTGTTGSATAVKLSVRYPATGTATFQWSQIIAESRTL
                     GLNPPTNTINLASGPRASIPQSVWTAFEQTAKGQDALIVLQRLTGSTLRPEIKTKIHF
                     ATNQLKGTVYYQSYGTNLVQNFSSTLSGQTVGGGQRFGAATLAIRPGATYPTVAAGYT
                     SSTDGPGCRVCHSAAAGGSVIVSNLHSSADSTLYRLGTDSASGGVGFPTVGDGRYGWP
                     GIYPDGSFIFSNSGPSPVYAVPGATPPGGLEDSAWCKTGSPSPCTSALTGTPPPNALY
                     SLSTSNIGTLITSAGIPTNLRATMPVFSPDGTRLAFNHYAGTVGSVTGDKRSLGMMDF
                     NNGTKTFSNFRRLVTQPTSACSSAFGTTDPCTNVWPTFLPGNEGVVFEREIFNNGRVS
                     GANHSDFGGTRSGCDGTGTCGDDGTKAELWWVSTDSTPTEARLNKANGRNADGSLYIP
                     TAADVSTAATAHSTTNEPILNYEPTMSPSAVGGYYWVAFTSRRLYGNVATVNPWWSDP
                     RFKPIGGGNGPTTKKIWVSAISTSATEGSDPSSPPFYLPGQEYLAGNSKAYWVLDACK
                     AASNTRSTANLCESDLDCCGAPSTAVCSLQTPISNPPKRHCIPVSTSSCIADDAATQC
                     TSDAQCCGFSTGSRCANGVCRLPPPLLLYTPGNYVRDFHAECPSGTHAVWQVFEWQAS
                     VPTGTSIDFVGSTASTAAGLSTAATIIAGTASPPSVSGWANNPVNLQSAFQTAGQTSR
                     EYLRIVMQLNPSSDRLSAPTITNWRVAYDCFDNE"
BASE COUNT         2471 a         4380 c         4315 g         2441 t
ORIGIN      
        1 ctcctgacgg caagcgggcc gcgcaagcct gcaccgggca tctcacggcc accggtgaaa
       61 tcgaggacct cagccagagc gccctcgttt tctacgacgt cagcctgagc ggcctgaagg
      121 agaccgagcg actcgatgtc gcacaactcg gggtcgaggg cttgcagaac gacgtggtct
      181 tcgcgaacgc ggagtacgcg ctaatcaaga gccagacgcc gtacggcggt acgagtcaca
      241 atcgctggat cgcgctcgaa ctcagcagca agaaggtgag cacgctgctg gaggcgcggc
      301 ccgacgagaa cggcgagggc aaagggctca cttacggcgc gatgagctgc tcgccgggct
      361 gctcggacgt gtgtctcttg gccgactccg accgcggcgt gctgcaacgg gtgcaaatcg
      421 aagcggggcg aagccccaag ttgctcacgc cggtcaccgt agaagagcag gtgggtcttc
      481 cacctcgtgg tatcgcgagc cgctgaagta cgcccggccc gacgaggaga acatcgagac
      541 tcgaaccatc tgcggttgat gatgatgagg aaagcgtgag gtcttgggct tggctgttcg
      601 gggttacccc cgtactcgcg gcgttctttc cgggccacct cgagctcgga agtccgaact
      661 tcgccaacgc cgcctacgcg acgcacgaaa catccagtcc ctccccccgg gacgtgacac
      721 ggatcgcctc cggttcactc atcagtgacg agctgctctt cgacttgtgt ccggagcggc
      781 tcgtcgccgt ctcttacgta gccgatcaag ctcacgtgac gatgctcgcc gggcgcgtcg
      841 cgtccaagct gccgcgtata accgccaatg ccgagcagat tctggcggta gagccagatc
      901 tggccgtgtt ctccgacttc agcagcatgc aagtagaggc gctggttcgg agcgcggggc
      961 actccgtggc gcgggtacgc gcaccccacg atttcgacga gctattggcg agcatccagc
     1021 tcctcggtcg gcaggtaggg gcaacgaacg ccgccgcaaa cctgtcgcgt gatctcacga
     1081 ctcgacttgc ggcgctcgcg cgggcacggc cccgcaagcc attccgcacg ctactcgtcc
     1141 agagtggcta cgcctacgcg gagggcacgt tgcaacacga ttgcctaaag cgcatcgggc
     1201 tcgacaacgc actcgagcct tggcacgtac acggtactcc cgcgctgcat caggagctat
     1261 tcatgctggc gcgccccgag ctcgtgttcg tggcgagtga gaagccggaa gtgcacagcg
     1321 aaagcggctc gaacctggtc gaacgcggga tacttccaga ggcggccgca cgcgtgcccc
     1381 taattttttc cgtgccggag acctggatgg ggtcactcac gcactacggg ttacgagcgt
     1441 gcgaagccta cgctcgagtg gccggtaggc tgccatgaac ccgcgttacc tctacgcggc
     1501 tctgactctg ctgctcatcg tgagcttgag cgcgtctatc gcactcggtg ccgtgccagt
     1561 gccgctgctc gccgcgctac gccccgggcc cgactgggac tgggtgcgac gaatcttgtg
     1621 gtccgtgcgg ctgccccgcg ctttgtgcgg gtgcatcgtc ggggccgcgc tgtcgagcag
     1681 cggcctcgtg ctccaaagtg tattccgcaa tcccttggct gaaccgagtg tactcggagt
     1741 gtccggctgc gcggcgctcg ccgcccaact cgcgctgttc agcggcctcg ggcaattggc
     1801 gagttactcg gtacctctag cggccgtgat tggagccgcg gtcgggatct cgctgcttct
     1861 attcctggta aggggcctgc accatgtacc ggagccggtg ctgatctcgg gggtagcgct
     1921 cggacagctt gcgctcgcgg tgagcgcact cttgctctcg ttggcgctcc gcgattacag
     1981 catcgcgagg cgactgctga cgtggttgct cggagggctg gatggccgca cgtggctgca
     2041 cgtgctgtgg ggcgtcggcc cgctcgtggc cgccattctg cgcatcgcgg cgcgctcaag
     2101 ggaaatcgat gcgctcacgc tgggtgacac gaccgcgctc gcggtgggag tggacgttgc
     2161 gcgctcgcgg cgcgagctgg tgctgattgc ggcagtgctc tccggcattg cggtggcgat
     2221 tgcgggcatc gtgggtttcg tagggctgat cattccctat ttcgtcaagc gctggttaca
     2281 gccgcgccgg ggcagcgcag tctgggcttg cgcgctggcg gggggcagct tcgtcgtgtt
     2341 gtccgacttg gtcgcgcgtc tcttgattgc tccggaagaa ctccagattg gagccgtgag
     2401 cgcgctcttc ggagcgccgc tcttcgcctg gttgctcttg cgtcgttacc gggagctcgc
     2461 cacgtgaact ccagcgtgat cgtgcgcccg ccgcgagcta gctcgctcga actccgctcg
     2521 atcgcggtgt ggcgcggcca gcggcaggtg ctccatgacg tgtgcttgca tttggctcct
     2581 ggtcagctgt gggcattggt cggcccgaat ggatccggta aaagcagctt gctggaggcg
     2641 ttgctcggcg cgctgccgtg ctcgggagaa atcttgctcg ggggacatga cgtgcgccgc
     2701 tggtcagctc gggagcgcgc ccgacacctc acgttgctcg ggcgagaatc gaccgcgcaa
     2761 actacgctac gggtgcgtga cctggtcgag ctcggccggt tgccataccg gcgcgcgttc
     2821 gacgctcttt cgagcgccga tcacagcgcg gtggactccg cgctgcaacg ggcaggcgta
     2881 ctcgaactgg agcaccgctc cgtcaatgat ctgtcggacg gcgagaagca gcgggtgttt
     2941 ttggctcgcg cgttcgccca gcaccccgag ctcattctac tggacgaagc cacgagtcac
     3001 ctcgacatct tgcatcgcga gcagaccctg caggggatcc gcgccttcgt ggccgaaggg
     3061 cgcttggcgt tgatggcaat ccacgatctc gagctggcgg tacgccatgc gagccacgtc
     3121 gcgatcctga gcgagggtcg catcgtggct tcgggcccgg cgctggacac gctgacgccc
     3181 acccgcctcg cccaagtgtt cggcatcgac gccgagattc ggcaagacga gcgaggcaat
     3241 caactgcgca tcttcggcct cgcggcttcg gagcgcaccc aaccgcgcag ctcgaacgga
     3301 cgacatccgt ccaattcgag cctgctcgag gtaaaaaccg tatgaaattg accttcgtga
     3361 tcggtggcgc acgctcgggc aagagtcgcc atgccatgca gctcgcacgc gccctgagcg
     3421 acaaccccgt gtatctggca acctcccgaa tctgggacga agaccatggt cggcgcatcg
     3481 agcgccaccg gaaagatcgc ggcccggagt ggcgcaccgt cgaagagccc aagtatctct
     3541 cccaggtagg cgtgcaagac gcggtggtcg tgatcgattg cgtcacgcta tggctgacca
     3601 acttcttcat cgatgtcggc catgacctag accggtgttt ggagcaggca tgggcggagt
     3661 tcagcaagct cgacacttgc cgcaatcact acatcgtggt ctccaacgag ctcgggcagg
     3721 ggctgcacgc cgagaccgag atcggacgca agttcaccga tctacaaggt atgatcaacc
     3781 agcgcatcgc tgcacgcgcg gacgacgtga cttggatggt tgctgggatt ccggtgtcca
     3841 tcaagcacac ggccccatcg tcgagaaccg aatagccgca tacgcacgac aaaaccgcca
     3901 tgctcaatct cccaactccc gatctcaatg cgctcgccgc cgcggagcag cgtcagtcac
     3961 gcctgaccaa acctcccggc agcttggggc gcctcgaaaa actcgtgttg cagttggcgg
     4021 ctttccaggc tcgccccctg ccgagcgcac gccccgccgc cgccctggtt tttgcggccg
     4081 atcacccggt aacgcggcac ggggtttccc cttatccgag cgcggtgaca gccgccatgc
     4141 tccacaattt tcgtgaaggg ggcgcggcgg cgagcgtgct cgcacgcgcg tgccgcgtcc
     4201 cactgtgtat cgtggacgtg ggtgtggcgg gcgtcagtga actgccgacc tcgtcgttga
     4261 gcaagggcgc cgtgctgcgc tcggatccgt tcggttcgcc ctcgagtttc gaggtcaatg
     4321 cacctgcgac cgcgcattcg ggagtctacg tatggcgtga atccgtggcg gactccaccg
     4381 ctggcgacct gcgcgtagaa gatgcgatgt ccgaggccgt gttcgagggc tgcgccgcgg
     4441 cgggccgtcg cgccgtaacg cgactcggtc cgctcgatgt gctgatcgtg ggtgagattg
     4501 gcatcggcaa cacgaccgcg gccgcggccg tgtgtgcggc gttgctcggg ggtgatcctg
     4561 gtgaactcgt gggcgccggc accggcgcga gcggggccat gttggagacc aagcggcgcg
     4621 tggtaaccga agccgtacag cgagtggctg acgagcgtag tccgctcgag attctgcgcc
     4681 gcctcggtgg gcgcgagatt gcggccgcgt tcggggccat ccatgccgcg ctcgagcagc
     4741 gcagcgtggt tctggtggat gggttcgtaa tcagtgccgc ggcgctcgcc ttggtgcgcg
     4801 ccaacgctcg agctctgcat ggcctggtgt tctcacatct atccggcgag cggggtcatg
     4861 cccgtgtgct cgcagagctc ggggttcaac ccatgctcga tctgggcctg cgcttggggg
     4921 aagccagcgg cgcgctcgcg gcctatccgc tgctcgaaca agcgtgtcta ttgcactccg
     4981 aaatggccac cttcgagtcc gctcaagtac ccgacaaggc ttgaccgaac gccttccctc
     5041 ccccactcgt ctgcgactcc caaactctgt ccgtctgcgc cctccgaatg actgcgcctt
     5101 ctctgcccac ctacggcctg cctccctatc tacgagggct ccgcgccgca ttcgtctttc
     5161 tgactcgctt gccggtcggc ggcttccctt atcaacgacg cgacttcgag tgggcagcag
     5221 cccatttccc aaatgtcgga ttcatcgtcg gtgcggcggg tggcctcgtc cattacctga
     5281 gccggggctt gggcgatatg gccgccgcgc tgttcgcgtt gggcaccacg gcatggctca
     5341 cgggtgcctt ccacgaagat ggcctagccg acacgttcga cgcactgggc ggctaccaca
     5401 accgcgagcg catcttcgag atcctcaaag atagccggat tggcagctat ggaagcgtgg
     5461 ccctcttcgt cgtgctcggc ctgcgcgtgg ccttgctcac gaacattgcg gccaaagctt
     5521 ccagccccta catcgcggca gcggcagtga ttgccgccca agtcttcggt cgggtgcccg
     5581 cggtgtggct gctcgcgatc acgccttacg tgtcccccga agcccaagcc aagggcagtg
     5641 gcgtcgcggg cgcgggcttc cctcaagccc tcgttgccac tgcgttcgcg gcgctgtacc
     5701 tgggcggtct aggtccattg ctcgagccgc cgctgctctt ctatccggcg ttgttcgccg
     5761 cgctcgccgt caccggtctg gtattgcgcc gctggttcct ggctcgggtc ggcggtgtaa
     5821 cgggagactt gctgggagct accgagcagc tcagtgaagt cgccgtcctg gtcgcttggt
     5881 cttgggcttt tgggggcatg gccgcaccgt aactcctccg ggggagttca gcgcgatgat
     5941 ggcctcccag cgctctcgcg tgaacagtgg cccacgtatt acctgattga gtcgatcgta
     6001 gtattcagcc aggcagcgat cctcgatcag gttgcgcccc tcttccaagc tcttcacgta
     6061 gccctcggga acatcccgcc ccagatgacc cgtacgccaa ccgcgatcgt atttgaacgg
     6121 gatgcgtgcc aacagaggat cggtcagcgc caacggatcg agcacgtgca cgtctggacc
     6181 agcgcccagc ccgaacagcc cgatattacc gaccacggcc acccgctctt tgcgctgtct
     6241 cgcctccacg ccgctcgtga ataggccgtc ttctctccaa gccgtcggcc gcacgttcat
     6301 gggaagcagc aggtgatctt gataccaaga gcgctcattc acgatgcgcg tatacgggaa
     6361 gtctcgcttc tccgtctctt tctcgagacg aaaaggtgag aacgtcaagc tcatgatcag
     6421 cgcgcccaag ccgagcgcga gcccctgcaa cggcagcccg gacgggccgc gcagcagctc
     6481 cccgccgaag tgcaccaaag ccgcggcgga gaccgcggtg gcggcggtca tgaaccgacc
     6541 gctcatgaaa tcgccaccca cggagaccac gtatgccaag ttcgcaatca acgccaaaag
     6601 cacgaattta gcgaggcgtt gggtgcgaat cgcctgaatt gaaatcgcga gcgcggctgc
     6661 aatcaggatg gcggtaaggg agtcattggc caccacgtcg atgaggtacg agaaaccctg
     6721 ccgcaccatt tctgcgcgag ggatatccgt gttgagcttg gcataagtcg tgttcgggga
     6781 cggaaaccca taatagatga tggcgaacac gaaccaggcg atggccgggg tggcgccgag
     6841 tagcacgcgc caccacagcc ctttgcggcg ccgctcctgc cacagagtcc atagcaaccc
     6901 gggcaacaac aacggtagcg cgtcgatgcg attgaccacc gtcagcgcgc tcagcagcac
     6961 gagctttccg ctactgcgca ccgcaggcct gaacaacacc gcgtagaagc agaacaggag
     7021 caagtgagtc aggctgttct cgaggccgct cgtagagaag tccacgaatg agcgagagaa
     7081 gcagagagca accaacacca gggcgccgag agctcggtcc cgtgccaccg caaacacgag
     7141 caccgaagcc gccagcgcgg aggtgacgac tccggccaac accgcgacca agtaatgatt
     7201 ctgggtcagc gcgagcggac ccgccaacac caacgcccac agcgggttcg tgatcccgag
     7261 cactcgctcc ccggcgttga agaccattcc gcgacccgct accaagtgat cgaccacacg
     7321 catggtgatg aacgcgtcgt cgcacatcca gcctcgacgc agtaccgcca tcacgaacac
     7381 gaccagcgcc agtaagcccc aagtcgtgcc ccgcgtggcg cgcgaacgct cactccattt
     7441 gaccggcgtc ggagactcgt tgaccttcaa ccagcccaat gcccgcaacc cgaggcggcg
     7501 ccatacacga attggcggcg cgtgcgcctg gccaacgtct ccgtcgcggg gtgcccgctc
     7561 ggcgtcggca cgcccccagg gaatccagcg gaggtagcta cgaagcgcag agtaattcag
     7621 ctgcatggtc ccaaacaatc gttcagcgtg cgacccgaaa gcctggcaga tacatcacgg
     7681 cccctagcta gctgaaacca cgacgtcagc aagctcgaca ttctgcaccc caccaccccc
     7741 gaccaagccc cggaaccaat cgcgccgacc gcctgcacta ccgcctgcac taccgcctgc
     7801 actaccgcct gcagagagca acttggatgc catgggagac gaggtccgga acatctagtc
     7861 tcgagcgtga gacctgtact gggaaacgga gcggtcatgg cctggtgggc gcgtaccatc
     7921 gcacgcttca gttccagctc cgcaagggtt tgcttgcacc aacctcggtc tgctcaggga
     7981 tcaacttggc acaccctgac acaagccccg cggagctagc ccatagtgaa ggtcgctagc
     8041 cgggccaagg tttagaacat ctacatattc gattaagaac gtttcatttt tctaatctat
     8101 cgagtgggcg catgctgcga ctcaggaggt cgacatgaca atcgattcat cgttcaagcg
     8161 cgctcgcccc gcgccaccgg aacatacctt cgaaggcgcc agtgccgctc ggcaatcacg
     8221 gttacccgat agcaacacgc aggcttggca gccccaaaag tacgagcaac gggcgggctt
     8281 cgtgcagagt ggcggcgtgc ccgtggtgga gctgctggct ccccgagcag gcgagcgcat
     8341 cttggatctc ggctgcggca cgggcaccct cacccagacg atcgcggagg cgggcgccga
     8401 agtgctcggc atcgataact cggaatcgat gatccaagcc gcccgcgcga aatatcctaa
     8461 tctcgccttc gagatccaga gcggcgagga gctcacttat gtcgatcgct tcgacgcagt
     8521 gttctcgaac gcagcgctgc actggatgca tcgcgcagaa gacgtcgcgc gcggcgttta
     8581 ccgcgccctc cgccccggtg gacgcttcgt ggccgagttc gggggagcga agaacgttca
     8641 ccacgtacgc gcggcggtgg atgcagccgc tcgagagttg ggcctgccca tcccgcagcc
     8701 ctacgcgcca tggtactttc ccaagctcgg agaatacacg agcttgctcg agaacgctgg
     8761 tttcgacgtg cgtttcgccc agcacttcga gcgccccagc ccgatgccgg atcaaggcgg
     8821 cgaaagcggc attgccagct ggctcgacat tttcgcgcgg cagctcttcc agatggttcc
     8881 cgcggctcag cgcgctcgct tcctcgcagc cgtggagcag cacgcgcgcc cgcagctgtt
     8941 ccgcgacgga acttggtgga tcgattacac gcgcctgcgc ctcgtcgcaa cgcggccgag
     9001 ctgaactgga tttcaaccga gcgcgggttt caaccgagcg cggtctcgac cgagcgcggg
     9061 tttccaccga gcgcgcagag ctctagagca agtccgccgt gaccaggccc aacttcccag
     9121 ttggatcctc gtaaaagagg cgcttgcagt cgacgctggg cgcggcaccc cgaaaggctc
     9181 cgagtagcgc aaccgacgag tagacggcgt cggggctggc ttcgaggaaa acggcggtgg
     9241 cccgagctga agccgggtcc cacaagaata gcgtttgcag atcgctagaa accccggtca
     9301 gttcaggatg actctccgcg ctcgcgtcga agcgctccgg caacgcgagc tcggtgccac
     9361 tcgaccacgg cgcaccactc gagaagcgac gcgccgcgaa cacggtatgc gttcggccac
     9421 cgccgaaccg agagtagaag agagtgcggt cgtcggcact gaggacaggc tctccgtagg
     9481 cttcggccgg ggagagcccg gactcagcgc tatcgttcag caattcgaat tcgaaagagt
     9541 tcgtgtctgc aaacgagtcc tgtcgtgagc ggcgctccca cgcttcgaag tggcggccgt
     9601 cggtagctac gaacaacacg cgcagcccat cggcactcaa cgctaccttg ccgggcgcgg
     9661 ccggaatgac ctgccaagtg ggcttttgaa atggctcatc cacggcgtct ctagccgcgc
     9721 tatacagcgt gacttggccg gcctgaacgc gcgtccacac gatgctcagt tcatccgcag
     9781 agacacttcc gagcctgcta tcggaagtca ccgcgagccc caacgagcgc ggcgtcccgg
     9841 cgcgcattcg catcgcgcaa acactctctg ggatgactat cggaccggga acggggccgt
     9901 ccgtctcgcc tccggcgccc gcgtctagct caggcgagcc gcccgcagct tcgctcgcgc
     9961 ctccggcgcc ccctgcggaa ccgacggggc tgccgccctg ctcagcaacg ccgccactcg
    10021 cacctgcgcg gtcaacgccg ccgctgccca actgagagct tctgctgccg ccctgcgtgc
    10081 cagcagatcc tcccgtcacg agcgagaccg gttcttgggt ggcccgttcg ccagagccac
    10141 aggccagcag cagcccgaaa gcaagcgcga aggtgacagc attattcatt gtcgaaacag
    10201 tcgtacgcca cgcgccagtt ggtaatcgta ggcgccgaga gtcgatcgga agacggattc
    10261 aactgcatca cgatgcgcag atactcgcgc gaggtctgcc ccgcggtctg gaacgccgac
    10321 tgcaggttga ccggattgtt ggcccagcca gaaacgctcg gcgggctcgc ggtgcccgcg
    10381 ataatggtcg ccgcggtgct caggcccgcg gcggtcgagg cggtggaccc aacgaagtcg
    10441 atgctcgttc ccgtgggcac cgaagcctgc cattcgaaca cctgccaaac ggcgtgggtg
    10501 ccgctcggac actcggcatg aaagtcacgg acatagttgc cgggcgtata gagcagcaag
    10561 ggcggcggta aacgacaaac cccgtttgcg cagcgagagc cggtggagaa cccgcagcac
    10621 tgcgcatccg aggtgcactg cgtcgctgcg tcatcggcaa tgcaagagct cgtgctcacg
    10681 ggaatgcagt gtcgcttggg aggattcgag atcggcgtct ggaggctgca caccgcggta
    10741 gaaggtgcgc cgcagcagtc caagtcactt tcacagagat tggccgtcga tcgcgtgttg
    10801 gaagcggcct tacaagcgtc taatacccaa tatgctttcg agttcccggc tagatactct
    10861 tgtcccggca ggtagaaagg tggactgctt ggatcactgc cctcggtcgc cgaggtgctg
    10921 atggctgaaa cccagatctt cttggtggtt ggcccgttgc cgccgccgat tggcttgaat
    10981 cgcggatcac tccaccacgg gttgaccgtc gcgacgttgc cgtacaagcg gcgcgaggtg
    11041 aacgcgaccc agtagtaacc gcccacggcg ctggggctca tggtcggctc gtaattgagg
    11101 atgggctcgt tggtcgtgct gtgagccgtg gcagcggtgg atacgtccgc cgccgtagga
    11161 atgtagagcg agccgtcggc gttgcgtccg ttggccttgt ttagacgcgc ttcggtcggg
    11221 gtggagtccg tggacaccca ccaaagctcg gccttcgtac cgtcatcacc acacgtgccg
    11281 gtaccatcgc agcccgagcg cgtgccgcca aagtcgctgt ggtttgcgcc gctcacgcgg
    11341 ccgttgttga agatctcacg ctcgaacacg acgccttcgt tgcccggcaa gaaagtgggc
    11401 cagacgttgg tacaagggtc ggtcgtgccg aacgccgagc tgcaggcaga ggtgggctgg
    11461 gttaccaagc gccgaaagtt ggagaaagtc ttggtgccgt tgttgaagtc catcatgccc
    11521 agcgatcgtt tatcgccggt cacgctgccg acggtgccgg catagtgatt gaacgcaagg
    11581 cgtgtgccgt ctggcgagaa cacgggcatc gtggcccgca aattcgtggg aattccggcg
    11641 ctcgtgatca gcgtgccgat gttactcgtg ctcagagaat acaaagcgtt gggcggaggc
    11701 gtacccgtca gtgcactcgt gcaagggcta ggtgatcccg ttttacacca ggcgctgtct
    11761 tcgaggcctc ccggcggggt cgcgcccggt acagcgtaca cgggagatgg gcccgaattg
    11821 ctgaatatga agcttccgtc gggatagatg cccggccatc cgtatcgccc atcccctacg
    11881 gtgggaaagc ccacgcctcc gctcgcgctg tccgtgccca atcgatacag cgtgctgtcc
    11941 gcggagctgt ggaggttgct cacgatcacc gatccgcccg ccgccgcgga gtggcacaca
    12001 cggcagcctg gcccatccgt ggaagaggta tagcccgccg cgacggtagg ataagtagcg
    12061 ccggggcgaa tcgcgagcgt cgccgcaccg aagcgttgcc caccgccaac ggtttggccg
    12121 gacaaggtag agctgaagtt ctgtactagg ttcgtaccgt aggactggta gtagacggtg
    12181 cccttcagtt gattggtggc gaagtgaatc ttggtcttga tctctggacg caacgtcgag
    12241 ccggtgagcc gctgcaacac gatcaaggcg tcctgcccct tggcggtctg ctcgaacgcg
    12301 gtccaaacgc tctgcgggat cgaggcgcgc gggccacttg ccaagttgat ggtattggtc
    12361 ggcggattca gcccgagggt gcggctctcg gcgataatct gcgaccattg gaaggtggca
    12421 gtcccggtcg ctggatatcg gaccgagagc ttcactgcgg tcgcgctgcc cgtcgtaccc
    12481 gtattccatt gtaccagcgg ggagagcaag cccaacggca acaccgtgtc ggcgtacgga
    12541 tagagcacct tggccgtatc tgccgaaccc acggtattcg cgaacgcgct cgtggcgctc
    12601 gcggacggtg aggcagtcgc atctagaaca ttcaggacca cattcaggtt gaatgtcgca
    12661 gacaacgatc ccgaatagcc agtcactctg atcgtgcccg cgttggggga taccaacgtg
    12721 agtaggcccg cgccgtcgat ggtcgcgata tcgaagcgat cgattgccca aactacgggg
    12781 ggcgtgcccg gataacagcc gctcggcgag agcgcggcgc tgaactggac cgagctcggg
    12841 gaaacgggtg aaaactgcgt gacggtgagg gttggagtcg tcgtcgtgac ggccagcgag
    12901 gtacaaggcg tcccgatcac cgaagtttcg gtctgcgtca gcgtcacccc ggagctcgtc
    12961 acctcgacat cgtcgccggg ctccacggtc aagccggtcg aatcatccgt gatgcggcta
    13021 cagaatgggt cgcaaggatt gttgcaatcc tggctggtac ccaagccttg gaggcgctgc
    13081 ttgccgatgg cctgaactcg cgtcccctgg gccgcgcatg cactccacag tccggagtta
    13141 caagtgcgca atccaaccga acaggtcgcg tacccggagt tctcgctttt cagggctcca
    13201 cactcgatgc tctgcccctc gatgctgcaa ggacagccct gatcgggagt cgcgcaagca
    13261 tctggccaca cttggacgag cggagcatca ccctcatagc tgcccggcgg caactcgctt
    13321 ccattgcacg cgaccgcaaa cgaaaacgag acagccaacc acgcgcgaga agacataggg
    13381 cctgccaagt ctgcgcccga accgtgagcg ggcgcgcgac gaccaagtgc acgaccccgc
    13441 acaaagcggg accgtacgtt ggcgtgaacg gctcgaacca acccgaacct aatgagagat
    13501 ggtgcggttc gcggtcgcgc aaaaaaacaa gtgggagctc gtccccggag ctcccaactt
    13561 gtccgctgtt acttgtccgc tgttgcttgt gcggtctggt ttttcgg
//
LOCUS       DASTKU010000876        13652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1959356, whole genome shotgun sequence.
ACCESSION   DASTKU010000876 DASTKU010000000
VERSION     DASTKU010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13652
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959356"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFQ61_30845"
     CDS             complement(<1..205)
                     /locus_tag="VFQ61_30845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9958943.1"
                     /translation="MADQKQRASEDLGRMMVQGVNSHAIFMLDCAGVVMTWNPGAERI
                     KGYKAEEIVGQHFSRFYRPEDVES"
     gene            complement(670..1335)
                     /locus_tag="VFQ61_30850"
     CDS             complement(670..1335)
                     /locus_tag="VFQ61_30850"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HET9958944.1"
                     /translation="MNRLTEPKGAEPILELSCIGEWAAWLTSNHHRSQGVWLHIPKKG
                     SKKALTYAEALEVALAWGWIDSQKRSFDARAWLQRFSPRKANSPWSKINRTKAEALIA
                     AGTMQAPGLAEVERAKRDGRWQRAYDGARVASVPSDLAAALALNARARAFFETLDSAN
                     RYAILYRVQTAKHPDTRVARIERFVEMCARQETIHPPRRAKAGTLAAARSPQTRRTTK
                     VQR"
     gene            complement(1338..1697)
                     /locus_tag="VFQ61_30855"
     CDS             complement(1338..1697)
                     /locus_tag="VFQ61_30855"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET9958945.1"
                     /translation="MSEQKTYRGSCHCGRVRFEVTAEISRASACNCSICSRTGWLMTS
                     VPAAQFKLVAGADAQTDYQFGKRTMHHLFCRTCGIRAFGKYVADDQESIIVNLRCLEG
                     LDVGALEVETFDGKSYA"
     gene            complement(1963..3522)
                     /locus_tag="VFQ61_30860"
     CDS             complement(1963..3522)
                     /locus_tag="VFQ61_30860"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9958946.1"
                     /translation="MIRTISVFCVVLFGMPACSRGDRGNHASPPLRPAAVQTTASAQL
                     GPTRPPPGRWRLTGFHSLHNVTLRISHILIRHKEVEDSRVSLSLFDWYTQYPGVTRDR
                     IEAWQLAERVARRAQQNPGSFAALAREVSEDPTTRERGGELGTVMASQLWNWPTVLDA
                     LAATSEGRVSEVVETEQGFHVFLRQAPAPERKLSGARILLTHTDAPFAGDVAVTRQPI
                     DRSRAEALKLSAELYQRLRDQPTAFPQLVRDHSNHVDAERGGDFGTWSTREATPLSRQ
                     VAVLATLQIGQVSAPIETVFGIEIIMRTADPPRPEYAADTLQFPFQPDDPASQLAAKN
                     AAEQAAARLAHEPRDFETMQRERHPSPLHVISGRDTPALEQALARLELGRVSPQPVLS
                     STRYLLLKRLPSSVLQPNPPPVTELPNPGRVELSKMVRYAQPAFLQVQFQSIAQQAST
                     SLGLDRETSLQLIRRHDEGANFTGLGPDAREQVIQRLGDELRQLLGTAAYEKYQTLAH
                     AHLEQALMSQR"
     gene            complement(3634..4017)
                     /locus_tag="VFQ61_30865"
     CDS             complement(3634..4017)
                     /locus_tag="VFQ61_30865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958947.1"
                     /translation="MNLFKIVSPIVAAGLILATAMDGDAAQCRRFPSASGTFNTYPTS
                     GEIRLLNMGCQDGTRGDLIADASPKEVRVTLRNKVTASARVRMIGSNSIGGGQCVAES
                     TTVGVTDSDPCGADVVKWVGELRND"
     gene            complement(4112..6238)
                     /locus_tag="VFQ61_30870"
     CDS             complement(4112..6238)
                     /locus_tag="VFQ61_30870"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9958948.1"
                     /translation="MNQGPLRQDPRTGVDSGASARPYRLTQAAPRAGAAALMLVVGAL
                     TAFYLSSSAEPERARSVTSASSASARPTPPVIRAGQPGLPQPRVRPAVYGKVQSSTAL
                     GIPHARVCALDVERSTPRAECVFTDGQGKFTLPAAEAALDMIATARGYLPGRASLSGS
                     ARTPFVITLVKGGVTVSGSVLDVGGGVVSGALVTARRAQHEPAVAFTRSDDTGRFELD
                     LAPGEVLLTAEAELYSAAHLTAEAPAQNVLLALAPASTIQGRVLARDGEGVADTTVTA
                     SVRAGLAELPSSTRTREDGSFEIAGLSAGAYELRAQAARWRSEVVLVRLGVAERRSEV
                     VLTAERAATLSARIRLEGKPCARGQLTVSGAEHSFATLDGAETVTLGALPFGPYEIEA
                     QCVGAYGKHEKLAIATDAPSVDWELAAALGSLRGRVIGANGNPLGRMSIELEPIGEPR
                     ARARSVCTSDERGAFECRGLPRGTYDCAIANGLSEPVRVAVEDGPSSEVLLRALATAT
                     LRVHVDAGEPGRSRPPVFARGVDPFALEPRWQDDAYVFERLRLGQYAVYVGGGLDGEP
                     AARATLDYEGQIVSLAIAVPRTVAVTGRVVDARGEPVPDTWVRAFASDSVEAGLGRAV
                     GQASLTDATGAFALHGLAPDTYQLHAAGSFGQARLSQIKGGSAGIRIQLEPLGPVAQV
                     ELGSPRALDSEGHSQAPASPSFAPPR"
     gene            complement(6305..7966)
                     /locus_tag="VFQ61_30875"
     CDS             complement(6305..7966)
                     /locus_tag="VFQ61_30875"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9958949.1"
                     /translation="MASPADGEVVHFPPFRLCPLREQLWREDELVAVRPKTFAVLWYL
                     ISHSQRLVTKEELLNEVWGAVNVGDGVLKTSLKEIRQALADSVTHPRFVETAHGRGYR
                     FIAAIAQPRRGSSSGRPAIGCANEPPEAASARVSCGRQPQLAQLRVALQRANTAQRQV
                     VFVSGEAGIGKTHLMKAFLQPLETQGEVVVARGQCVEQSSGVERYLPVLEALSRLCRA
                     PGGELLLGLLQREAPTWFARFAWARSPDRAGSLVAPFESARGGRRVRELADALDSFTR
                     DRTLVLLLEDLHWADAATLDLIHHLAQRPDPARLLVLATYRPLDGYVENHERLIHMDR
                     HLHQRGSCESLGLPCLDEAQLQSYLLQRFMSQGLVDTVLPIIRRRTSGNPCLVNQLLE
                     AWISRGWLSNTAGEWRLIGELQGLRCELPSAIIDLVEAEKERLSPFQQNLLEVASLAG
                     AEFCPVVVALALRLDVTRVEAECLRWARHGRIVRTPAHAQGSGPGTAFEFVHELFQQV
                     ISERLGAARRSRLHQLLLQATQRRAALEPHSVVLANDEARLSRVS"
     gene            8272..8904
                     /locus_tag="VFQ61_30880"
     CDS             8272..8904
                     /locus_tag="VFQ61_30880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958950.1"
                     /translation="MWNWRFGRALLLVSMFVAGACNEGEPVGVDCSAHPRCGVDEAGC
                     ESPSECFSIPGCGGGLCIPGAQACRETCGTASCAVLESYPAQIACDGRKPAPGRNGSG
                     GNTAAGGAASSGGSTVDCSELNENLSGELRAARACSSDAECGQPIAGTSCGCTRNLVA
                     RSDADLTRVQALLEELQRNECDAGLISTCDCPAADGFRCTSGACTWNYTR"
     gene            9003..10613
                     /locus_tag="VFQ61_30885"
     CDS             9003..10613
                     /locus_tag="VFQ61_30885"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET9958951.1"
                     /translation="MRTATELTARLVELSSLFEANVHAGTLDHCIVLTEPSQLAGLCA
                     EQQLLATEAARLRNVEGYVLTLQPSRVAAALESVHDRSLRKAIYEAYFTRASDHGPRA
                     GHFDNTPVLDEVLELRYQLARLKGCQNYAELALQGGPITSPDLAERYLLSAEPDLKAK
                     AQAALDEIWAFAKQQGAPRGFASWDLWYYASWLVAEKVGLSASREKQLFPLSSSVHGV
                     LAFAERLFGVSFEPRSTARALCYGILAEDGSSLGELRLSLEDGERTGGGRRVALQEDP
                     SHSPASRVLDVHCGLVSALEGPKLLSFRAVGALFETLGEGLARLLVCSKASEDRPPAK
                     LIALVTAAVFRHCARHFEVLSPCTSDGGGGFDAEMHAQVLRRFDFASALERSQSLELA
                     LFDLRIHRDYVPTTKATQLRAQVLDTFAQVRRERSVLPPSYWTRFANTATEIFTAGRG
                     ATLWQRTWAEKAAENLTGRLAAEGFSAPARRWLREALRSAAAGADWAEVETALELPGD
                     AGAIGGRRGSGESPTGYSPNTSVVSSSS"
     gene            complement(10574..10861)
                     /locus_tag="VFQ61_30890"
     CDS             complement(10574..10861)
                     /locus_tag="VFQ61_30890"
                     /inference="COORDINATES: protein motif:HMM:NF039672.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6156 family protein"
                     /protein_id="HET9958952.1"
                     /translation="MTSSTDGTVCRLFATYSGVRLPLRLVGPLEPDSIAHRNTFMRGY
                     FDAAERLMLCEKVVYGQVELSHRYEYADSGALARAVITTQEDEETTLVFGE"
     gene            complement(11057..11584)
                     /locus_tag="VFQ61_30895"
     CDS             complement(11057..11584)
                     /locus_tag="VFQ61_30895"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET9958953.1"
                     /translation="MRATLGTFQSGRDRVTIDSFPLRLVISRRREALVARAPEAPDAV
                     LEPSLSGREQAVLAGVRRGLSNKRIAIELACSEATVSRNVRRALGKLGPNREWLRSEQ
                     SAAPPAGRRATRLLTAAERAILIDVLRGCSSAEIAARRRRSIRTVQNQIAGLFGKLGV
                     NSRRELCARIACSPR"
     gene            11796..12506
                     /locus_tag="VFQ61_30900"
     CDS             11796..12506
                     /locus_tag="VFQ61_30900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958954.1"
                     /translation="MHSRATNRTPPIPRTMPPPPSTKWTAILHLALLASACGGDAMDL
                     EGSSEVTGQTESAIDLDISGQQVLGCDKVSANATVTLKAFVPSIQKTSSGDYGGVFCK
                     RYVVDVNVERGVPAGYSPYFTIDGYGVDRPHLPLPFDACDSYREELTVYSKILGDFEK
                     IGGGTRRGQSVALHIGPTGEIFPAHCALVESDSFQEIDPQHRCQPGEFCQKYRVAVRA
                     YDVVTGVEQAVRVRVAHQ"
     gene            12853..12990
                     /locus_tag="VFQ61_30905"
     CDS             12853..12990
                     /locus_tag="VFQ61_30905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958955.1"
                     /translation="MKWPEAPWTVDRHEPRGVAAIAAALFLLFDRYRDARAVVMEEYN
                     D"
     gene            12983..>13652
                     /locus_tag="VFQ61_30910"
     CDS             12983..>13652
                     /locus_tag="VFQ61_30910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HET9958956.1"
                     /translation="MIEETTLREVIVAKKTIEAHNMASFELVPADGQSLPDFTPGAHI
                     DVTIPGGFTRQYSLANPSSERHRYLIGIWRDANSRGGSRTLYDSVNEGDRLRVSPPRN
                     RFRVPRGTARALLFARGIGATAVLSIADYLKSSQVPFEFHYVFSGLSLGSFKTLIDSS
                     PYAEDTRYYSDLSEHNQLLDASAVLADRPANTHLFICGADWWQDPIIKLAKETFGFDD
                     ARVHS"
BASE COUNT         2466 a         4446 c         4348 g         2392 t
ORIGIN      
        1 cgctctcgac gtcctcgggc cgatagaagc gcgagaagtg ctgccccaca atctcctcgg
       61 ccttgtagcc cttgattcgc tcggcccccg gattccaagt catcacgaca ccggcgcaat
      121 ccagcatgaa gatggcgtgg ctgtttaccc cctgcaccat catccgaccg aggtcttcgg
      181 atgctcgctg cttttgatcg gccacggcca gctcctgact gtctccccga cgcgcctccc
      241 cgaccccaca ctagttctca gtcgtcgcgg taccggacga cggtaaacat gtgctgatcc
      301 aacgagtgga ttgcctgcgg gggtcggata cctccatatc gacggactcc ggtccgagat
      361 tctactcccg agacacctga tcagggatgg aaaatctcgg ccttcccacc aagcaccgag
      421 tacggcagtc gtgcctgctg aatcggacag ttacgcggca taaacgagcc ccacgtcaca
      481 gccgatcggg ggtggctgcc gcggaagcag cgctgcgctt ccgcgttcga agcgctcgat
      541 atcgactcgg caaaggactc tggtccgcca acactaccgt tcacgagcga atctcgccga
      601 tcgatgtcgt gttccgtgtc gaatgactgg cagcggggcg cgtccgcgat atcgacgcgc
      661 ttaggctcgt tagcgctgca ctttcgtcgt ccgacgtgtc tgcggcgagc gcgcggcggc
      721 tagggtgcct gcctttgcgc gacgcggtgg atgaatcgtt tcttgccgtg cgcacatttc
      781 gacgaagcgc tcgatgcggg ccactcgtgt gtctggatgt ttggcagttt gaacacgata
      841 gagaatcgcg taccggttgg cgctatcgag cgtctcgaag aacgctcgtg cccgggcatt
      901 tagcgcgagg gccgcagcga gatcagaggg tacgctcgcg acgcgcgcgc cgtcgtaggc
      961 ccgctgccag cgtccatcac gcttggcgcg ctcgacttcc gcaagaccgg gagcctgcat
     1021 ggtgcccgcg gcgatcaacg cctccgcctt cgtacggttg atcttcgacc acgggctgtt
     1081 cgccttgcga gggctgaagc gctgcagcca cgctcgagcg tcgaacgagc gcttctggct
     1141 atcgatccat ccccacgcga gcgcgacctc gagcgcttct gcgtacgtga gcgctttctt
     1201 ggaacccttc ttcggtatgt ggagccagac gccttgcgag cggtggtgat tggacgtgag
     1261 ccaggcggcc cactccccga tgcaagagag ctcgaggatc ggttccgctc ccttcggttc
     1321 agtgaggcgg ttcactttca ggcgtaactc ttgccgtcga aagtctcgac ctcgagtgct
     1381 ccgacgtcga gaccttcgag gcaacgcagg ttgacgatga tgctttcctg atcatctgcc
     1441 acgtacttgc cgaacgcgcg gattccgcag gtgcggcaga acagatggtg catggtcctc
     1501 ttgccaaact ggtaatcggt ctgcgcgtcc gcgcccgcca ccagcttgaa ctgcgccgcc
     1561 ggcacggagg tcatcaacca acccgtccga ctacaaatcg agcagttgca cgcgctcgcg
     1621 cgagagatct cggcggtgac ctcgaagcga acccgcccac agtggcacga tccccgatag
     1681 gtcttctgtt ccgacatatt ttggttcaca cgcttactcc gggccgagct tcgcgtattg
     1741 gaaaagtgcg acacatcgag cagtcccccc tatgggcacg ctcgatcagc tgccggcctc
     1801 caaacgggcg cctgcctcgc gggcgcgttc gttggattgg agttgcacgg tgcgacctgc
     1861 gcggtggggg cgactcgcca cgtgcgttta ggtctgcgct ggggagtgcc cggtcgatca
     1921 gtcgcgcccg ttcacgcgag gccggcatca tcccagaaag actcagcgtt ggctcatgag
     1981 cgcttgctcg aggtgggcgt gggcgagggt ctgatacttc tcgtaggcgg cagtaccgag
     2041 tagctgtctc aactcgtcgc cgagcctctg gatgacctgc tctcgtgcgt ccgggccaag
     2101 cccagtgaag ttcgcacctt cgtcgtggcg acggatcagc tgcagagacg tctctcgatc
     2161 gagcccgagt gaggtactcg cctgctgcgc gatactctgg aattgcacct gtaggaacgc
     2221 cggctgggcg taccgaacca tcttcgaaag ctcgacccga ccgggattgg gcaactcggt
     2281 gacaggtggg gggttaggct gcagaaccga gctgggcaag cgtttgagca ggaggtagcg
     2341 cgtgctactg agcacgggct gcgggctcac tcgacctagc tcgaggcgag ccaatgcctg
     2401 ctcgagcgcc ggcgtatcgc ggcccgatat cacatgcagg ggactggggt gacgctcgcg
     2461 ttgcatcgtc tcgaagtctc ggggctcgtg cgcgaggcgc gcagcggcct gttcggcggc
     2521 gttcttggcg gccaactggg acgccggatc atccggctga aaaggaaact gaagcgtgtc
     2581 ggcggcgtac tcgggccttg gcggatcggc tgttcgcatg atgatttcga ttccgaacac
     2641 cgtctcgatt ggcgctgaga cttggccaat ctggagcgta gcgagaaccg caacctgacg
     2701 cgacagcggt gtggcctccc gcgttgacca ggtcccgaag tcgccgccac gctcagcgtc
     2761 gacatggttg gagtggtctc gaaccagttg cggaaaggcg gtgggttgat ccctcagccg
     2821 ttggtagagt tccgcggata gcttcagcgc ctcggcccgc gagcgatcga tcggctggcg
     2881 agtgaccgcc acgtccccgg caaagggagc gtcggtgtgc gtaagcagga tgcgagcccc
     2941 gctcagcttg cgctccgggg cgggagcttg ccgcagaaag acgtggaagc cctgctcggt
     3001 ttcgacgacc tcgctcacgc ggccctcgga ggtcgcggcc agcgcatcca acacagtggg
     3061 ccaattccag agctgggagg ccatcaccgt cccgagctcg ccacctcgct ctcgcgtagt
     3121 cgggtcttcc gagacttctc gtgccaatgc cgcgaagctt ccggggttct gctgcgcccg
     3181 acgagcaacg cgctcagcga gttgccaggc ttcgattcga tcgcgcgtga cgcccgggta
     3241 ctgcgtgtac caatcgaaca agctcaggct gaccctcgag tcctcgacct ctttgtggcg
     3301 gatgagaatg tgcgagatcc gcagggtcac gttgtgtaac gagtgaaagc cggtcaagcg
     3361 ccagcgcccc gggggcgggc gcgttggccc cagctgggcc gacgccgtgg tttggacggc
     3421 ggcgggtcgt aatggcgggg acgcgtggtt gccgcggtct ccacgactgc aggccggcat
     3481 cccgaataga acgacgcaga atacgctgat ggttcgaatc attgcgttcc ccgaaagctc
     3541 gccgtcaccg atgggccgtc agcgcgtcac cccgtagtgg actgcaagtg cgcgacaacg
     3601 gctgacgacc caccattcac ttctatctat ccctcagtcg ttgcgcagct cgcccaccca
     3661 cttgacgacg tccgcgccgc aaggatcgga gtcggtgacg ccgaccgtcg tggactcggc
     3721 cacgcactga ccgccgccga tgctgttcga gccgatcatg cggacgcgcg cgctcgccgt
     3781 caccttattg cgcaaagtca ccctcacctc cttgggactc gcgtccgcga tcaggtcacc
     3841 ccgcgtgcca tcctggcagc ccatgttgag cagccggatt tccccggagg tggggtaggt
     3901 attgaaggtg cccgatgcgc tcgggaaacg acggcactgc gcggcgtcgc cgtccatggc
     3961 agtggcgagg atgaggccgg cggcaacgat ggggctcacg attttgaaca gattcatggg
     4021 ttcgtcctct ctttttcgat ctcttcagga tcggtcgccg cgggcacgac cgatgaagtg
     4081 ttggcaaacg agcgcgacct cgcgctgctc gtcagcgcgg tggggcgaag ctcggtgacg
     4141 ctggagcttg agaatggcct tcggagtcca gcgcccgagg cgagccgagc tcgacctgtg
     4201 cgacgggccc gagcggctcg agctgaatgc ggatgcccgc actgccccct ttgatttggg
     4261 agaggcgcgc ttgtccgaag ctcccggcgg cgtgcaattg gtacgtgtcc ggcgcgaggc
     4321 catgcagcgc gaaggcaccg gtggcgtccg tcagtgacgc ctgacccacg gcgcggccca
     4381 gacctgcttc tacagagtca gaggcgaacg cgcggaccca cgtgtcgggc accggctctc
     4441 cacgcgcgtc cactacgcga ccggtaacgg caaccgtgcg cggaaccgca atggccagac
     4501 tcacgatctg accttcgtag tcgagtgtgg cacgcgctgc cggttcaccg tccagcccgc
     4561 cgcccacgta gacggcgtac tgacccaagc gcagacgctc gaaaacgtag gcgtcgtctt
     4621 gccagcgcgg ctcgagcgcg aacggatcca caccgcgagc gaatacggga ggcctggagc
     4681 gcccgggctc accggcgtcc acgtgcacgc gcagggtcgc cgtcgccagc gctcgcaaca
     4741 acacttcgga cgatggcccg tcctcgactg cgactcgcac gggctcactc agtccattgg
     4801 cgatcgcaca gtcgtaggtg ccgcgcggca ggcctcgaca ctcgaacgca cctcgttcat
     4861 cgctggtaca gacgctacgc gcgcgggcgc gcggttctcc gatgggctcg agctcgattg
     4921 acattcggcc caaggggttg ccgttcgctc cgatcacgcg cccccgtaag ctgccgaggg
     4981 cggcggctag ctcccagtcc acgctcggcg catccgtcgc gattgcgagc ttctcgtgct
     5041 tgccataagc gccaacacac tgcgcctcga tctcataggg gccgaaaggc agcgccccga
     5101 gcgtcaccgt ctcggccccg tccagggttg cgaacgagtg ctcggcgccc gacaccgtga
     5161 gctgacctcg agcgcagggc ttgccctcca aacggatgcg ggcgctgagc gtcgcggctc
     5221 gctccgcagt gaggacaacc tccgaacgtc tctctgcgac ccccagccgc accagcacca
     5281 cctcgctgcg ccagcgcgcc gcttgcgcgc gcagttcgta cgcgccggcg gagagtcccg
     5341 cgatttcgaa gctaccatcc tcgcgggtcc gcgtagagct cggtagctcc gctaggccag
     5401 ctcgtacgga ggccgtcacc gtggtatccg cgaccccctc accgtcgcga gccagcacac
     5461 gaccctgaat ggtagaggcg ggcgcgagcg caagcagcac gttctgggcg ggcgcctccg
     5521 ccgtcaagtg agccgccgag tagagctcgg cctcggcggt caacagcacc tcaccgggtg
     5581 cgagatctag ttcgaaacgg ccggtatcgt cggaacgggt gaaggccacg gcgggctcgt
     5641 gctgagcacg tcgagcggtc acgagcgcgc ccgagactac gccgccgccg acgtccagca
     5701 ccgagccgga gacggtgaca ccacccttga ccaaggtgat gacgaacggc gttcgagccg
     5761 atcccgagag tgacgcgcga cccggcaagt agccgcgagc cgtggcgatc atgtcgagcg
     5821 cggcttcggc agcgggcagc gtgaacttgc cctgcccatc ggtgaatacg cactcggcgc
     5881 gcggggtgct tcgttccaca tccagcgcgc agacccgggc gtgcggaatc cccagagcgg
     5941 tgctggactg caccttgccg taaaccgcgg gccgcacgcg gggttgcggg agacctggct
     6001 ggcccgctct gatgacgggc ggcgtgggcc tcgccgacgc gctcgaagcg gacgtcacgg
     6061 agcgtgcgcg ttcgggctca gccgacgagg acagatagaa agccgtcaga gcgccgacca
     6121 cgagcatcaa tgctgccgcg cccgctctcg gtgcagcctg agtgaggcga tacgggcgcg
     6181 ctgacgctcc tgaatccacg ccagtgcgcg ggtcttggcg caagggcccc tgattcatgc
     6241 ccacgatcat agcgagtggg tcgacttgaa atcatgagga cgttctggaa aaaccgtgaa
     6301 agcttcatga aacccggctc agccgtgctt cgtcgttcgc gagtacgaca gagtgcggtt
     6361 ccaaggccgc gcgccgctgc gtcgcctgca ataacagctg gtgtaagcgt gatctccgcg
     6421 cggcgccgag ccgctcggag atcacctgct gaaagagttc gtgcacgaac tcgaaggccg
     6481 tgccagggcc actcccctgg gcgtgggccg gagtgcgaac gatgcgccca tggcgagccc
     6541 agcgcaggca ctccgcttcg acccgtgtca cgtctagccg gagcgccagc gcgaccacca
     6601 ccgggcaaaa ctccgcaccc gccagactcg ccacctcgag caggttctgc tggaacggac
     6661 tcaatcgttc cttctcggcc tccaccaggt cgatgatcgc gctcggcaac tcgcagcgta
     6721 acccctgcag ttcaccaatc aagcgccatt ccccggccgt gttcgacaac cagccacgcg
     6781 aaatccaagc ctctaagagc tggttcacga gacagggatt gccgctggtg cgtcgccgaa
     6841 tgatcggcag taccgtatcg accagacctt ggctcatgaa gcgttgcaga agatacgact
     6901 gcagttgcgc ctcatccaga cagggcaacc ccaatgattc gcatgagccg cgctgatgca
     6961 ggtgccggtc catatgaatg aggcgttcgt ggttctcgac gtatccgtcg agcggtcgat
     7021 acgtggcgag cacgagcaag cgcgccggat ctggcctttg agcgagatga tgaatcagat
     7081 ccagagttgc cgcatcagcc caatgcaggt cctcgagtag caggacgagc gttcgatccc
     7141 gagtaaagct gtcgagagcg tccgccaatt cgcgcacccg ccgcccacca cgagcgctct
     7201 cgaacggggc cacgagcgag cccgctcgat cagggctacg tgcccaagcg aaccgcgcga
     7261 accaagtggg ggcttcgcgc tgaagcagcc ccaacaacag ctcgcccccg ggtgctcgac
     7321 aaaggcgact caaggcttcg agcacgggca gatagcgctc gacgccgctc gactgctcga
     7381 cgcactgtcc acgagccact acgacctcac cctgggtctc cagcggttga aggaaagctt
     7441 tcattaggtg agtcttaccg atgcccgctt cgcccgacac gaacacgact tgacgctggg
     7501 cggtgttggc cctctggagt gcgacgcgca gctgagcgag ctgcggttga cgaccgcacg
     7561 aaactctcgc cgatgctgct tctggtggtt cgttcgcgca tccgatggct ggacggcccg
     7621 agctcgaccc gcgccgcggc tgcgcgatcg ccgcaatgaa ccggtatccc cgcccgtgcg
     7681 cggtctcgac gaagcgcggg tgagtgacgg aatctgcgag tgcctgacga atctctttga
     7741 gtgaggtctt cagcacgccg tcacccacgt tgacggcgcc ccaaacctca ttcagcaact
     7801 cctccttcgt gaccaagcgt tgcgaatggg aaatcaggta ccacagcacc gcgaaggtct
     7861 tcgggcgcac cgccacgagt tcatcttcac gccagagctg ttcacgcagg gggcacaacc
     7921 gaaacggtgg gaagtgaacg acctctccgt cggcagggga tgccatatcg tgaggcaata
     7981 ccgcgccgaa agaccgaaga tctagcactc gattgcagag ccactctcct ctaatcgcgt
     8041 tgtatggaca tcagtcgttt gtatcgacat ctcttgtata gacaccgatt cctcgtatcg
     8101 acatcggacc ttgcaagtag aacctttcag attggaggaa ggtaggaggc gagccctttg
     8161 cgagagagaa ggcgtccgag cgatcgcccg cgaagcgtgg tatcgccgta gcctggtcga
     8221 atcgagcgcc acccacggtc gccccgcgtc tcggtgctac actcaggccc tatgtggaac
     8281 tggcgatttg ggcgagccct gcttttggtt tcgatgttcg tggctggagc gtgcaacgag
     8341 ggagaaccag taggtgtcga ttgcagcgcg catccacgct gcggggtgga cgaggcggga
     8401 tgcgagagcc cgtcggagtg cttcagcatc cccggctgcg gcggcggcct ctgcatcccc
     8461 ggcgctcagg cttgcaggga aacttgtggc actgcgagct gcgcggttct cgagagctat
     8521 ccggcccaga tcgcgtgtga tggccgtaaa ccagctcccg gacgaaacgg cagcggaggg
     8581 aacacggcag ccggaggcgc cgcttctagt ggcggctcga cggtcgactg ctcggaactg
     8641 aacgagaact tgtccggcga gctccgagcc gctcgagcgt gcagctccga cgctgagtgc
     8701 ggacaaccga tcgcggggac cagctgcggg tgcacgcgca acctggtagc acgcagcgac
     8761 gcggatctca cccgcgtgca agctctcctc gaagaactgc aaagaaacga gtgcgacgcg
     8821 gggctgatta gcacgtgcga ttgccccgcc gcggatgggt tccgctgcac ctctggggcc
     8881 tgcacctgga actacacgcg ctagttgccc gcgacggttc tccaggagat ttggcgcgcg
     8941 cgatcggacc gcctggacgc gcaggcgaca ctggtccggc tcttgcctag gccctggagg
     9001 tcatgcgcac ggcgacggaa ctcacggcaa gattggtcga gctttcgagc ctcttcgagg
     9061 cgaacgttca cgcgggaacg ctggaccact gcatcgtatt gacggagcca agccagctcg
     9121 ccgggttgtg cgcggagcaa cagctgctgg caacggaagc ggctcgtctt cgaaacgtgg
     9181 agggctatgt tctcaccctg cagccatccc gggtcgcggc cgcgctcgag agcgttcacg
     9241 accgctcgct acgcaaggca atctacgagg cgtacttcac tcgtgcatca gaccacggcc
     9301 ctcgcgccgg ccacttcgac aatacccccg tgctggacga ggtcttggag cttcgttatc
     9361 agctcgctcg cctgaaaggc tgccagaact acgctgagct tgcgctccaa ggtggtccca
     9421 taacgagccc ggacctcgcg gagcgctatc tgctttccgc agaacctgac ttgaaggcca
     9481 aggcccaggc cgctctcgac gagatctggg ccttcgccaa gcaacagggt gcaccccgtg
     9541 gattcgcgag ctgggatctt tggtactacg catcgtggct cgtagcagag aaagtgggtt
     9601 tgagcgcctc gcgcgagaag cagctcttcc ccctctcctc cagcgtccac ggcgtactcg
     9661 ccttcgctga gcgcctgttc ggcgtgtcct tcgagccgag atccaccgcg cgggcgctct
     9721 gctacgggat cctggctgag gatgggagct cgctcggaga gttgcgactc agtctagaag
     9781 acggcgaacg aaccggcggt ggacgccggg tagctttgca agaggaccct tctcattctc
     9841 ctgcgtcgag agtgctcgac gtgcattgcg gcctggtttc agccttggaa ggcccgaagc
     9901 tgctctcttt tcgggccgtg ggtgcgctct tcgaaactct gggcgagggt ctggcgcgac
     9961 tattggtgtg ctccaaggcc tcggaggacc gtcctcccgc gaaactgata gcgctcgtta
    10021 ccgcggcggt ctttcgccat tgtgcacgac acttcgaggt gctcagtccg tgtacttcgg
    10081 acggaggcgg cggcttcgac gccgaaatgc acgcgcaagt gctgcgcagg ttcgacttcg
    10141 cctcggcact cgagcgctcg cagtccctcg aactggcgct gttcgacctg cgtatccacc
    10201 gggattacgt gcctacgacc aaagcgacgc agctgcgcgc tcaggtattg gacacgttcg
    10261 cccaagtgcg tcgcgagcgc agcgtcttgc cgccatctta ctggacccgc ttcgccaaca
    10321 cagccacgga gatcttcacg gctggccgag gcgcaacgct ctggcagcgc acgtgggcgg
    10381 agaaggcagc ggagaacctg accggacgcc tcgccgccga aggcttttca gcgcccgctc
    10441 gacggtggct gcgcgaggca ctgcggtcgg ccgcggccgg cgccgactgg gccgaagtgg
    10501 agaccgcgct ggagctgccg ggcgatgcgg gcgcgattgg cgggaggaga ggatcgggtg
    10561 agagcccgac cggttactcg ccgaacacca gcgtagtctc ttcatcttcc tgagtggtaa
    10621 tcaccgctcg cgccaacgcc ccggagtcgg cgtactcgta gcgatgagat agctccactt
    10681 gtccgtacac caccttctcg cacaacatca ggcgttccgc ggcatcgaag taacctcgca
    10741 tgaaggtatt gcggtgagcg atgctatccg gttcgagtgg ccccacgagt cgaagaggca
    10801 ggcgaacacc gctataggtc gcgaacaagc gacatacggt cccgtccgtg gatgatgtca
    10861 tggcgcgcag cctgtctccg agaaggcagc tccgcaagca cggatcttat gtccgaaaca
    10921 cgacagcggc cgacacactt gtcgtctttg cgacaagctc gatcacagcg gagtcgtgca
    10981 aagcgcggcg tgtaggccgg tgacgatgca acgtgcgagg aaccggtgtg tcgagcctcg
    11041 gcggacgttc ggggaatcag cgcgggctgc acgcgatacg cgcgcagagc tcacgacgcg
    11101 agttcacgcc gagctttccg aacaacccgg caatctggtt ctgcaccgtc cggatcgagc
    11161 gtctgcgccg cgccgcgatc tcggcgctcg aacacccgcg aagcacatcg atgagaatgg
    11221 cgcgctcagc cgcggtgaga agccgagttg cccgccgccc cgccggcggc gcggcactct
    11281 gctccgaccg cagccactcg cggtttgggc caagcttacc gagcgcgcgc cggacgttgc
    11341 gcgacacggt cgcttccgag cacgccagct cgatggcgat acgcttgttg gagagccctc
    11401 gccgcacccc cgcgagcacg gcctgctctc gcccggacag gctcggctcg agcacggcgt
    11461 cgggtgcttc aggcgcgcgg gcgacaagtg cctcgcggcg ccgcgagatg acgagccgca
    11521 acgggaatga atcgatcgtg actcggtctc tcccagattg gaacgtgccc agggttgcgc
    11581 gcatgttggg taatacgcag cgtcatggcg agatctctcg cgcggagctc acgagtgctt
    11641 ccgcaagaac tactcatgac cgtggagcgc cggggggcca cagtctcgag caagcggacg
    11701 ccgaacccgg agcacactgg caagcgtcag tgctcgcggg gcggcggcct aatcacgcgc
    11761 gcgcgccaag caagcgcccc acgaaaggca agagcatgca ctcaagagcc accaatcgca
    11821 cgccgccgat cccgagaacc atgccgcccc cgccgagcac gaagtggacg gcgatccttc
    11881 acctcgcctt gctcgccagc gcctgcggcg gtgacgcaat ggacctcgaa ggttcgtccg
    11941 aggtgacggg gcagaccgag tcggccatcg acctcgacat cagtgggcag caagtactcg
    12001 gctgtgacaa ggtctccgca aacgccacgg tcacactcaa ggccttcgtt ccttcgattc
    12061 aaaagacgag ctccggggat tacggcgggg tcttctgcaa gcgctatgtc gtcgacgtga
    12121 acgtcgagcg cggcgtgcct gctggctaca gtccttactt caccatcgac gggtatggcg
    12181 tggaccgtcc ccaccttccg ctaccgttcg acgcctgcga cagctaccgc gaggagctga
    12241 ccgtttactc gaagatcctc ggcgacttcg agaagattgg tggcggcacg cgtcgcgggc
    12301 agtccgtggc cctgcacatc ggtcctacgg gggagatctt cccggcgcac tgcgcgctcg
    12361 tcgagagcga cagcttccag gagattgacc cgcagcatcg ctgccagccc ggagagttct
    12421 gccaaaagta tcgagtcgcg gtgcgagctt acgatgtagt gaccggcgta gaacaagccg
    12481 ttcgcgtgcg ggtcgcgcac cagtgaagct cgccccgagc gcgctttggg cgggccgcga
    12541 ccagcgactt cggtattgac gatcatgcac ggcgggggac caaaaggcga ccgctcttca
    12601 gctccagatg cgacagacga ttgtcggatc tgcgacagct cccgatcatg atgcctaggg
    12661 tttccccgga ttgtgcggcc tcctacaaag cccgtacaag agtttcgttc tgcacgctct
    12721 ccgccccggt cggtctcgca tgagctgcgc tcgcatcgtt ttcggcgcgc ctgacgagct
    12781 catcgcaact accggagcgg aacgagtgag cgcacggggc gccccgaaac ggaaccaaaa
    12841 cgcagccaaa acatgaaatg gccggaagcc ccatggaccg tagaccgcca cgagcctcgc
    12901 ggtgttgccg cgatagcggc ggcactcttc ttgctgttcg atcgttaccg tgacgcccgc
    12961 gccgtagtga tggaggagta caatgattga agagaccacc ctgcgtgaag tcatcgtagc
    13021 caagaagacc atcgaagcgc acaacatggc ctcgttcgag ctggttcctg cagatggaca
    13081 aagtctgccg gacttcaccc caggggcgca catcgacgtg accattcctg gcggcttcac
    13141 acgccagtat tcgctcgcga atccctcgtc ggagcgtcac cggtacctga tcgggatttg
    13201 gcgggacgcc aacagccgag gcggctcgcg gacgctctac gatagcgtga acgaagggga
    13261 ccgacttcgc gtgagcccgc cccgcaaccg tttccgcgtt ccgcgcggaa cggcgcgcgc
    13321 cctgctcttc gcgcgcggca tcggagcaac ggcggtgctg agcatcgccg actacctgaa
    13381 gagctcgcag gtcccgttcg agttccatta tgtcttctcg ggcctatcac tcggctcctt
    13441 caagacactc atcgactcgt cgccttacgc agaggacact cggtactact ccgatctctc
    13501 ggagcacaac cagctcctcg atgcgtccgc ggtgctcgcc gacagaccag cgaataccca
    13561 cctcttcata tgtggcgcag actggtggca agatcccatc atcaagttgg ctaaagaaac
    13621 gtttggcttc gacgatgcgc gcgtccactc gg
//
LOCUS       DASTKU010000877         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_393368, whole genome shotgun sequence.
ACCESSION   DASTKU010000877 DASTKU010000000
VERSION     DASTKU010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393368"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFQ61_30915"
     CDS             complement(<1..215)
                     /locus_tag="VFQ61_30915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958957.1"
                     /translation="MMRFDHWALHPARLLHDHLLVALRQSPALAQTLFGVRLPPPKRG
                     DGLWDYTTLVLCKASRRYIRKGMRVWDL"
     gene            complement(259..2184)
                     /locus_tag="VFQ61_30920"
     CDS             complement(259..2184)
                     /locus_tag="VFQ61_30920"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HET9958958.1"
                     /translation="MQLWLIGAECTRPPSYSTSICALAVTELLRDSLDGCQWSLAASL
                     SVSPQEANTDMYILGLATMEESAAVLMCDGRVVAAAEEERFSRRKHHCGFPYESIEFV
                     LGAGGVSLAEVDEVAVYWDRLRFGPRVQCGLSTTLRSLRSPSLISTKLRRWAEVWRGS
                     AGPESGWSSLFHTRRTLRRRFGRSPRRIRYFDHHRCHMASTYFASDFEQSAILIMDGA
                     GEAACTTWARGDGLALSRLDEHRVPHSLGHFYSAVTGYLGFAMLDGEYKLMGLSPYGE
                     PSYVGWIEQNFLRRLGGGRYALVNDALDYHRALTGRFEGAFSDRFGPPRRGDDDEVTD
                     RDRDIAASAQLAFERTVLEMTRELRRRTGLDRIALAGGCALNSVANGRVLRDGDYAQV
                     YVPPVPHDSGGALGAAMLCHVELTHRRPEPLHHALFGSQFGDRAVEAALAFHPNELEA
                     EKLSEDELIARCARALADGSVVAWFQGAMEFGPRALGSRSILADPRRDGMRDVLNAVV
                     KKREPFRPFAPAVKAERASEFFELSQPSPFMSLVVPVRAEKRAQLAAVTHVDGTARPQ
                     TVDLQTCPKFWKLLDEFERLTGVPVLLNTSFNIQEPIVRTPEQALRTYLVSGIHALAL
                     GSHWVTRADAVRAEGRA"
BASE COUNT          478 a          745 c          691 g          384 t
ORIGIN      
        1 aggtcccaaa cacgcatgcc cttccggatg tagcggcgcg acgctttgca aagaaccaag
       61 gtcgtataat cccaaagccc atcaccgcgt ttgggtggtg gcaagcggac gccaaagagt
      121 gtttgcgcaa gcgctgggct ctggcgcaac gcgacgagca ggtgatcgtg cagtaagcgc
      181 gcagggtgca gcgcccagtg atcgaatcgc atcatggtgg atcgcttccg ttgggcgcct
      241 cgcgagcgcc gctgtctttc aggctcggcc ttcggcgcgc acggcgtctg cccgggtgac
      301 ccaatggcta cccagcgcaa gcgcgtgaat cccggagacc agatacgtcc gcagcgcttg
      361 ctccggcgta cgcacgatag gctcctggat gttgaacgat gtattgagca acaccggaac
      421 gccagtcaga cgctcgaatt cgtcgagcaa cttccaaaac tttgggcaag tctggagatc
      481 gaccgtttgc gggcgtgcgg ttccgtccac gtgtgtcacg gccgcgagct gcgcacgctt
      541 ctcggcccgc accggaacga cgaggctcat gaaaggagaa ggctggctga gctcgaagaa
      601 ctccgaggcg cgctcggcct tcactgccgg cgcgaaaggc cgaaacggct cacgcttctt
      661 taccacggcg ttcaagacat cacgcatccc gtcccggcgc gggtccgcca agatgctgcg
      721 gctgccgagc gctcgcggac cgaactccat tgcgccctgg aaccaggcga cgacggaccc
      781 atcggccaaa gcacgcgcgc accgcgcaat tagctcgtcc tcgctcagct tctcggcctc
      841 cagctcgttc gggtggaacg ccaaggcagc ctccaccgcg cggtccccga actgcgaacc
      901 gaagagggca tgatgaagcg gctcgggacg acgatgtgtc agctcgacgt gacacagcat
      961 cgccgcgccg agcgcgccgc cagagtcgtg cggaacaggc ggaacataga cctgagcgta
     1021 gtctccgtca cgtagcacgc ggccgttcgc cacggagttg agcgcacagc cgcctgcgag
     1081 cgctatgcga tcgagccctg tacggcgtcg caactcccgc gtcatctcga gcacggtgcg
     1141 ctcgaaggca agctgagccg aagcggcgat gtcgcggtcg cgatccgtta cctcgtcatc
     1201 gtcaccgcga cgtggtggcc cgaaacgatc gctgaatgcg ccttcgaagc gcccggtgag
     1261 cgcgcgatgg tagtccagcg cgtcgttgac caacgcgtag cgccccccgc caagacgccg
     1321 taagaaattc tgttcgatcc agcccacgta actcggctcc ccgtagggac tcaagcccat
     1381 cagcttgtat tcgccgtcga gcatcgcaaa gccgaggtac cccgtcaccg ccgagtagaa
     1441 gtggcccagc gagtgaggca ctcggtgttc gtccaagcgg ctaagagcaa gaccatcccc
     1501 ccgtgcccac gtcgtgcacg cggcttctcc tgcgccatcc atgatcagga ttgcggactg
     1561 ctcgaagtca ctggcgaaat aggtgctggc catgtgacaa cggtgatgat cgaaatagcg
     1621 gatccgtcta ggggaacgcc caaagcgcct gcgcagcgtt cggcgcgtgt gaaataggct
     1681 gctccagccg ctttcagggc cggcggagcc tctccaaact tcggcccagc gccggagttt
     1741 ggtcgaaata agtgaagggc ttcgcaaact tcgcaacgtc gtggacaaac cgcactgaac
     1801 gcgagggccg aatcgtagac ggtcccagta gaccgccacc tcgtcgacct ccgccagact
     1861 cacgccacct gcgcccaaca cgaattcaat tgattcgtag ggaaagccgc aatgatgctt
     1921 gcgtcgacta aagcgctctt cttcagctgc ggcgaccacg cgcccgtcac acatcaacac
     1981 cgccgcggac tcttccatgg tagcgagacc aagaatatac atgtcagtgt tcgcttcttg
     2041 tggtgacacc gagagtgaag cggccaagga ccactgacac ccatcgagtg aatcgcgcag
     2101 cagctcggtc accgccaacg cacagattga agtcgagtaa ctgggtggcc gcgtgcactc
     2161 ggctccaatg agccaaagtt gcacgactca cacatttccg cgaagcgcgc cggtcagtgt
     2221 tcgcggccgt aaacggagct tatccgtcga aagtcgccaa cgcagagcca tgtgcgccca
     2281 gacgaaattc catgggga
//
LOCUS       DASTKU010000878         4190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1064414, whole genome shotgun sequence.
ACCESSION   DASTKU010000878 DASTKU010000000
VERSION     DASTKU010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064414"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..2034
                     /locus_tag="VFQ61_30925"
     CDS             163..2034
                     /locus_tag="VFQ61_30925"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9958959.1"
                     /translation="MKGYLTEPCGPKTASEPARASFSPVRFRRGVAFAAAALTCALGC
                     TATITDDRGSNAGAPNGASGGSNVASGGSNASSGGKGTVGNGGTGPGGSAGGTTNNAN
                     GGSTSPSAGTTSTDPPPVGGELSNCATPGPQLIRRLNKEQFRNTLVAAFNNDQSVPQT
                     DVIANPYDLYFHVDADSQVIRDLDAGLLMDYADEVADWAVTNNKLGTFNSCTTVDDNC
                     AKNFISAAALKLMREPLPNDRLQAYITAFKTETAFKDAATLVLSAMIQSPYTLYRREI
                     GTSDSGAFKLSQYDIASELAYFLTDSPPDQQLLADAQANKLTTQAAIDSHAARLLQTP
                     AAKAAMKRFVEGWLEVEKLRVKTKETKVYDLTMDMREAMIAETTDTFVDAFNNGGDVA
                     KLFTTQDTFVNQSLAQFYGLNGGGSTSTKVSLAGTKRATGLLGNAAFLTAHAQPENSS
                     PTQRGIVVRGRLLCQAIPPVPQGLNTALAAPGVAKTNRERYAMHSQNQPCNGCHKVLD
                     PAGLVFENYDGFGRYRDNENGVAIDASGGLVGIPDGDITLNGVQSLNDFLSKSDTVRA
                     CLVRYWSYYAHGRDNWTDKKCNDDSVRREAAANGYTLKSVLMGILHAPSFTRRVKSL"
     gene            2062..3390
                     /locus_tag="VFQ61_30930"
     CDS             2062..3390
                     /locus_tag="VFQ61_30930"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9958960.1"
                     /translation="MGNLSRRDFARNAALVALCSPFINLLQPSRARAATGKAKYFLLF
                     VTNGRDPSAWKHSGSDSSLTLCAQTEPLSPIKSNLTIIEKLNSNGTAASHGSPGGLCG
                     NTWGASQLISIEQFVSDSLKANGVNTQVPNIVLGGAEGNQPTTFWRANNAITPIYSPV
                     TAFQALFGNFSGGGGTTTGAGGASSGDTAAADHLKKRKKSMLALINGDINLLKSSLGT
                     EEQKKLQLHLDSLSQLETRINQTMTGGTGGGGSTQPSAGCKAPAKPTLQSQPFLNSSV
                     HLDLAINAFACDITRVAAVQFGHHQMCPVDVEGAKGDYHNDFMHSDPAPHSRLANCEK
                     YLAGQFLAAANKLKAIPAADGSGTLFDQTIMLWARDMGDAPNHTGDDMAYVFSGGAGG
                     YLKAGGRFINGNGASHQRVFFNVCEAMGITNFSKFGDAAQSHDPLAGVGA"
     gene            complement(3668..>4190)
                     /locus_tag="VFQ61_30935"
     CDS             complement(3668..>4190)
                     /locus_tag="VFQ61_30935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958961.1"
                     /translation="PKAAATQQLQQFLTEAKFRRNDKVAAYFTRAFLDKVAIDSLRRA
                     TQAAKSNGDKLIKDLEKKGTSKHWDRDQKGKYVQSHLNATEWRALELSIGVEEKKLTP
                     REAAALFYADWAVEVFGNVNDVWLESTSVSSQGCFEGYVTAADHDGTMRRYVVHMHHE
                     VGEWLVEFVKQMR"
BASE COUNT          823 a         1403 c         1220 g          744 t
ORIGIN      
        1 ccaaacgcat ttgcgccgca tcggtacgcc cgggtttact gctagcctca acatttccag
       61 cctggcactt actaaggaaa cgcaatgagt gcctcggatg cattgaagca cttgcatctg
      121 cgcacgccgc gctacgctat agccccgcga aagaaaggct gaatgaaagg ttatcttacc
      181 gaaccttgtg ggccgaagac tgcatcggaa cccgcgcgcg cttcattctc ccccgtccgc
      241 ttccgccgtg gcgttgcgtt tgctgctgcg gcgcttacct gcgctttggg ttgcacggca
      301 acgatcaccg atgatcgcgg cagcaatgcg ggcgcgccca acggtgcgtc gggcggctct
      361 aacgtcgcgt ccggtggctc gaatgcctcg tctggcggca agggtacggt cggcaacggc
      421 ggtactggtc ccggcggcag tgcgggcggt accaccaaca acgcaaatgg cgggagcacg
      481 tcgccctctg ccggcacgac cagcacggat ccccctccgg tcggcggtga gctttcgaac
      541 tgcgccacac ccggccctca gctgatccgt cgtttgaaca aggagcagtt ccgcaacacc
      601 ttggttgcgg ccttcaacaa cgaccaaagc gtcccgcaaa cggacgtgat cgcgaaccca
      661 tacgacctgt acttccacgt cgatgcggac tcccaagtca tccgcgatct cgatgcgggt
      721 ctgctcatgg actacgcgga cgaggtcgcc gattgggcgg tgaccaacaa caagctcggc
      781 acgttcaatt cctgcacgac cgtggacgac aactgcgcga agaacttcat cagcgcggct
      841 gccctcaagc tcatgcgtga accgttgccg aacgatcgat tgcaggcgta catcacggcc
      901 ttcaagaccg agaccgcttt caaagacgcc gcgaccctcg tgttgagcgc gatgatccag
      961 tcgccgtaca cgctgtatag gcgcgagatt ggcacctccg attccggtgc tttcaagctc
     1021 agccagtacg acatcgccag cgagctcgcg tacttcctca cggactcgcc gcctgaccag
     1081 cagctcctcg ccgacgccca ggccaataag ctcacgaccc aggcggccat cgattcgcac
     1141 gcggcgcgcc tgctccagac gccagccgcc aaagcggcca tgaagcgttt cgtcgaaggt
     1201 tggctcgagg tcgagaagct gcgggtcaag accaaagaaa ccaaggtcta cgacctgacg
     1261 atggacatgc gcgaagccat gatcgcggag acgaccgaca cgttcgtcga cgctttcaat
     1321 aatggcggcg acgttgccaa gctcttcacg acgcaagaca cgttcgtgaa tcaaagcctc
     1381 gcccagtttt acggactgaa cggcggcggc tctacctcaa cgaaggtgtc gctcgcggga
     1441 acgaagcgcg ccaccggttt gctgggtaac gctgcgttcc ttaccgccca cgcccagcca
     1501 gagaactcgt ctccaacgca gcgaggcatc gtcgtgcgtg gccgcttgct ctgccaggcg
     1561 atcccgcccg ttccgcaggg cttgaacacg gccctcgcgg cgcccggcgt ggccaagacg
     1621 aatcgcgagc gctacgcgat gcactctcaa aaccagccgt gcaacggctg ccacaaagtg
     1681 ctcgacccgg ccgggctcgt gttcgagaac tacgacgggt tcggtcgcta tcgcgacaac
     1741 gagaacggcg tggccatcga cgcgagcggt gggctcgtgg gcatcccgga cggagacatc
     1801 acgctgaacg gcgtccagtc tctgaacgat ttcctttcga agagcgacac cgtgcgcgcg
     1861 tgcctggttc gctactggtc ctactacgcg cacggccgcg acaactggac ggacaagaag
     1921 tgcaacgatg attcggtccg tcgagaagcg gcggccaacg gctacacgtt gaagagcgtg
     1981 ctcatgggca tcttgcatgc cccgagcttc acccgccgcg tcaagtcact ctaagtcgag
     2041 cacagggagc aactccgcaa aatgggcaat ctatctcgcc gagacttcgc acgaaacgcc
     2101 gcgctggtcg cgctctgctc gccgttcatc aacctgctgc agccgagccg cgcacgagcc
     2161 gccaccggca aggccaagta cttcttgctg ttcgtcacga acggcagaga cccttctgcc
     2221 tggaagcaca gcggctccga ctcgagcctc acgctctgcg ctcagaccga gcccctctcg
     2281 ccgatcaaga gcaacctgac catcatcgag aagctgaaca gcaacggcac cgccgccagt
     2341 cacggttcgc ccggcgggct ctgtggcaac acctggggcg cgtcgcagct gatttcgatc
     2401 gagcagttcg tgtcggactc gctgaaggcc aacggcgtca acacccaggt gccgaacatc
     2461 gtgctcggtg gcgcggaggg caaccagccc accacgttct ggcgagcgaa caatgcgatc
     2521 actccgatct acagccccgt taccgctttc caagcactgt tcggtaactt ctccggcggc
     2581 ggtgggacca cgacgggcgc gggcggcgcg tcttctggtg atacggccgc ggcggaccac
     2641 ttgaagaagc gtaagaaaag catgctcgcg ctcatcaacg gcgacatcaa cctcctgaag
     2701 agctcgctcg gcactgaaga gcagaaaaag ctccaacttc acttggattc gctctcgcag
     2761 ctcgagacgc gcatcaacca gaccatgacc ggaggaaccg gcggcggtgg gtccacgcag
     2821 ccttcggccg gttgcaaagc gcctgccaag ccaaccctcc agtcgcagcc gttcttgaac
     2881 agctccgtgc atctggatct cgccatcaac gcgtttgctt gcgacatcac gcgcgttgcc
     2941 gcggttcaat tcggccatca ccagatgtgc ccggtcgacg tcgaaggagc caagggcgac
     3001 taccacaacg acttcatgca ttccgacccg gcgccccact cgcgcctcgc caactgcgag
     3061 aagtacctcg ccggacagtt cttggccgcc gcgaacaagc tgaaggcaat tcccgccgcg
     3121 gacggcagcg gtaccctgtt cgaccagacg atcatgttgt gggcgcgcga catgggagac
     3181 gctcccaatc acaccggtga cgacatggcg tacgtcttct cgggcggtgc cggtggctac
     3241 ctgaaagccg gtggccgctt catcaacggc aacggcgctt cgcaccagcg cgtcttcttc
     3301 aacgtctgcg aggccatggg catcaccaac ttctcgaaat tcggcgacgc agcccagagc
     3361 cacgatccgc tagccggcgt cggggcgtaa cgaagagtca cgagcgcggc cgaacagcgg
     3421 cgcggacgaa agcacagacg ggcgctgcgc cattcctagt gaagggccag cgcccgttgt
     3481 cattccatcg aactaagggg tgtcccaagt tccccgccct cttgcgagcc gagatcctgg
     3541 ggctgcgaag cacggcctcg tcgctccact cctcggaggg cttaagccga cctccggtgc
     3601 tcgcccctcg ggcgcgcttc gctcgcgttc ctgggctctc gcgacgggca ggaacttggg
     3661 acactcccta gcgcatttgc tttacgaatt cgacaagcca ctcaccgacc tcgtggtgca
     3721 tgtgcacgac gtaccggcgc atggttccgt cgtgatcggc agccgtcacg taaccttcga
     3781 aacagccctg tgagctgacg ctggtgctct cgagccaaac gtcattgacg tttccgaaca
     3841 cctcgaccgc ccagtctgcg tagaagagcg cggccgcctc gcgcggcgtg agcttcttct
     3901 cctccacgcc gatggacaac tccagcgcgc gccactcagt cgcattgagg tgagactgca
     3961 cgtacttccc cttttgatcg cggtcccagt gcttggaagt tcccttcttc tcgagatcct
     4021 ttattagctt gtcgccattg gacttcgccg cctgcgtagc gcgccgcagg ctatcgatgg
     4081 caaccttgtc gaggaaggcg cgagtgaagt acgcggcgac cttgtcattc cggcgaaact
     4141 ttgcctccgt gaggaactgc tggagttgct gcgtggcggc cgctttaggt
//
LOCUS       DASTKU010000879         5482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_171691, whole genome shotgun sequence.
ACCESSION   DASTKU010000879 DASTKU010000000
VERSION     DASTKU010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171691"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VFQ61_30940"
     CDS             complement(<1..428)
                     /locus_tag="VFQ61_30940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958962.1"
                     /translation="MTEANDAIAAANTELDRVAVRLAALHGELDEIFLLHQECLMLGH
                     LELAEQVLAGFAELLALHAQHEETWFLPLYEVVGVEARFPLVLYRGQHRKMLGMVRSF
                     QARTAELSRNRTMKPSELRRRVLALFDEHARFKHLLEHHDG"
     gene            complement(436..732)
                     /locus_tag="VFQ61_30945"
     CDS             complement(436..732)
                     /locus_tag="VFQ61_30945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958963.1"
                     /translation="MPRMCEICAVLQAPPSAGARPKLRRKLFGERLVVLCAEHAERVD
                     STAVSTLEELRALFLEEGGRRSLLDRRAPLDRRAFPPRPEGRRKQGRRGASDLR"
     gene            complement(764..1735)
                     /gene="gshB"
                     /locus_tag="VFQ61_30950"
     CDS             complement(764..1735)
                     /gene="gshB"
                     /locus_tag="VFQ61_30950"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HET9958964.1"
                     /translation="MRFLFIMDPFQNLAWDKDTSFAFMRGAARRGHQIWHCLPQDITN
                     RGQDVYAKATELRVHEGAPWLSAENQEAELELAHLDCVWIRKDPPFDRGYLFLTHQLD
                     LVTDRVLVMNAPRGVRDANEKMFAFQFVEHMPRSMVTADPKRILAFVQEVGGRAVLKP
                     LDGAGGVGVVVLAAGDQNARSLADIWTLEGKQLALVQEYQPAVRVGDKRVILLDGKCL
                     GAILRVPRPDDVRANIHAGGRVEPTTLTPAEAKLVADVGPKLREHGLWFVGLDLIGER
                     LIEVNVTSPTGIQELGRFNGRPAEDDVVAWVEERSSAMKLARRADLR"
     gene            2005..3741
                     /locus_tag="VFQ61_30955"
     CDS             2005..3741
                     /locus_tag="VFQ61_30955"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET9958965.1"
                     /translation="MSAKSSISIGASCLRGLRSPGLWATGLAMALLLSAPQGEAKDEK
                     VAGRAAAATEHPLATRTALSVLRQGGNAADAAVAAALMAGVVSPSSSGLGGGGFALVY
                     SPKLAAPVALDFREVAPQGVDAAILDRRPLPPEQRGALVGVPGEARGLEWLQQRFGSL
                     SFASLVAHAEQRARQGFAVGPHLARTLAGSASDLTRDASLSSVFFPGGRAAAAGRVVR
                     NPRLAKTLSRLAQQGAQAIYSGPIAAEIARTVQQYGGSLNEHDLTNYAAIERPPLKVT
                     WGEDDVFTMPAPSAGGLLLTQTLSLFSKAELQHAANPQGLPRPAAESGLYLHLLGEAF
                     RASFVDRMAHVGDPALVPDPAPRLLDAAHLNELRKRIAIDRTHSIPLLNTEEHGTHHL
                     VVADAQGMVVSLTTTVNRTFGAKIVTPDSGLVLNDQLDDFALSRDVDHLGAGSRVAPN
                     APRPGARPVSSMTPSIVLRAGRPILAIGGSGGLTIGTNVTQLLIAHLALGQAPKDMVQ
                     RPRIYVPMGIKETLLVDSGFSDAVIEDLTFRGERLAPMKFTGSAVQLLAWDSRGWSAA
                     ADPRKFGEAATQ"
     gene            complement(3843..4688)
                     /locus_tag="VFQ61_30960"
     CDS             complement(3843..4688)
                     /locus_tag="VFQ61_30960"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET9958966.1"
                     /translation="MNCRTVVPWLEPFVDGELSPEKVVEVEQHVQGCRVCAERVRLGQ
                     AFRSSLRRVSEPLALPSDAFRERLKLALEAAREREWEAHVVARQAESNRMLSWRTILP
                     VAAAAGFTLFWAASDPSSRRLERNETASHANASVVNADQVIEQLIDHHVHDVPQASDL
                     ALLPSDGAQFEREVGVPVRIPSLKQYGGLLEGGTVVPVHNNQRAASLRYKVSGHRVTL
                     YVYDPSRVPLEQRLEKRLVANAPVYVGARRGYWIGATERAGVGYALASDLNDAETAEL
                     VAALR"
     gene            complement(4685..5335)
                     /locus_tag="VFQ61_30965"
     CDS             complement(4685..5335)
                     /locus_tag="VFQ61_30965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9958967.1"
                     /translation="MMARVSHRQEFEGEVLEHLPSLLAVANRLMRNGSEAEDLVQDSV
                     LKAIRARDQFESGTNLKAWLLKILTNTFINRYRRGGLERSVLDGPDADPLADGWVSAA
                     TMEALRDPESQALRRILEAEIHQALQELPTDFRLAVVLSDIEDLSYKEISDIMGCPIG
                     TVMSRLHRGRRILKSRLYEHALALGIVGPEAAVGAANAEQPVVEMETYRKRKGARA"
BASE COUNT         1043 a         1853 c         1632 g          954 t
ORIGIN      
        1 ccgtcgtggt gctcgagcag atgtttgaag cgagcgtgtt cgtcgaacag cgccaacacg
       61 cgccgtcgca attcactggg tttcatggtg cgattgcggc tcagctcagc cgtgcgtgcc
      121 tgaaacgagc gcaccatacc gagcatcttt cgatgttggc cacggtaaag caccagcggg
      181 aaacgagcct ccacccccac cacttcgtac agcggtaaga accaggtttc ctcgtgctgc
      241 gcatgcagcg ccaagagctc cgcaaagccc gccaaaacct gctcggccag ctccaagtgc
      301 cccaacatca ggcactcctg atggagcaga aagatctcat cgagctcccc gtggagcgct
      361 gccaggcgca ctgcgacccg atcgagctcc gtattagcgg ctgcgatcgc gtcattggcc
      421 tcggtcatgt cttcctcaac gcaaatcgga cgcccctcgg cgtccctgtt tcctacggcc
      481 ttcagggcgc ggagggaaag cacgtcgatc gagcggagcg cgtcgatcca acagcgaccg
      541 ccggccccct tcctccaaga acaaggcgcg aagctcctcc aaagtcgaga ccgcggtgga
      601 gtccacccgc tccgcgtgct cggcgcacaa gaccaccagc cgctcgccaa acaatttgcg
      661 ccgcaacttg gggcgcgcgc cagcgctcgg cggagcctgc aaaaccgcgc aaatctcgca
      721 cattcgaggc atggctaacc tccgggaaag ggagtctcga cggctaccgc aggtcagcac
      781 ggcgggccaa cttcatggca ctggatcgct cttcgaccca cgccaccacg tcgtcctccg
      841 cgggccgtcc attgaatcga ccgagctcct ggatccccgt cgggctcgtc acgttgacct
      901 cgattagccg ctcaccaatc aaatcgagcc ccacgaacca aagcccgtgt tcgcgaagct
      961 tggggccgac atcggccacg agcttcgcct ctgcgggcgt aagggtggtc ggctccaccc
     1021 gacccccagc gtgaatattg gcgcgaacgt cgtcggggcg gggtacgcgc aagatcgcac
     1081 ccaggcactt gccatccagc aagatcaccc gcttgtcccc caccctcacg gcaggttggt
     1141 actcctgcac gagagccagt tgcttgccct cgagcgtcca aatgtccgcc agcgagcgag
     1201 cattttgatc accggccgca aggaccacga cgccgacccc gcccgcacca tccagtggct
     1261 tcaataccgc tcggcctcca acctcttgca cgaatgccaa gatccgtttg ggatctgcgg
     1321 tgaccatcga acggggcatg tgttcgacga actggaacgc gaacatcttc tcgttcgcgt
     1381 ctcgcacccc gcgcggcgca ttcattacga gcacgcgatc agtcaccagg tccagttggt
     1441 gagtgaggaa caggtaaccg cgatcgaacg gcggatcctt tcggatccag acgcagtcga
     1501 ggtgagccaa ctccaactcc gcctcttgat tttctgcgct caaccagggt gctccttcgt
     1561 gaacgcggag ctcggtggcc ttggcataga catcttgacc acgattggtg atgtcctgtg
     1621 gcaagcaatg ccaaatctgg tgcccgcgtc gcgcggcgcc gcgcatgaag gcaaagctcg
     1681 tgtccttgtc ccaggccagg ttctggaacg ggtccatgat gaataggaag cgcacggcgg
     1741 gagcatagcg cgattcctcg ccgcggtacg gaatcgtgcc ctggcaatcc gtgcgacgtc
     1801 ggataagctg gttcgtgaga cctatgcccg caatctcgat aacgcgcgcc cgccaaattc
     1861 ggcgtttgga agaggctctc gtgaagaccg aagcggagcg tacgtcccgg cgcggaaccc
     1921 ggacggcagc ccaatgaagc tcagacccga aatcggcgtg agaggtggca gtccgagcgc
     1981 cctggagtgc gggagacatc gtccttgagc gcgaagtcgt cgatctcgat tggcgcttca
     2041 tgtctacgcg gcttacgcag cccgggactc tgggcaaccg ggttggcgat ggcgctactg
     2101 ctctcggcgc cccaaggcga agccaaggac gaaaaggtgg ctggtcgcgc cgcggccgct
     2161 acggaacacc cgctggccac gcgcaccgca ctcagcgtac ttcgccaagg cggcaacgcg
     2221 gcggatgcag cggtcgcggc ggcgctgatg gcaggagtgg tgagcccgtc ttcgagcggg
     2281 ctcggcgggg gcgggttcgc gctcgtctat agccccaaac tagccgcgcc ggtggcgctg
     2341 gacttccgag aggtggcgcc ccaaggcgtc gatgccgcga tactcgacag acgtccttta
     2401 ccccccgaac aacgtggtgc gctggtcggt gttcccggtg aggcgcgcgg cctcgagtgg
     2461 ctgcaacagc gtttcggcag cctcagcttc gcgagcctgg tggcgcatgc ggaacagaga
     2521 gcgcgccaag gcttcgccgt cgggccgcac ttggcgcgca ccctggccgg tagcgcgagc
     2581 gatctgacgc gggacgcgag cctgagctct gtgttttttc ctggcggtcg cgccgccgcc
     2641 gcgggccgcg tcgttcgcaa tcccaggttg gcgaagacgt tgtcccgcct cgcgcaacag
     2701 ggcgctcagg ccatctactc ggggccaatt gccgccgaaa tcgcgcgcac cgtgcagcag
     2761 tacggcggca gcttgaatga acacgatctc accaactacg ccgccatcga gcgccccccg
     2821 ttgaaggtga cgtggggcga ggacgacgtg tttacgatgc ccgccccctc cgcaggaggg
     2881 cttctcctga cgcaaacctt gagcctcttc tccaaggccg agctgcagca cgccgcaaac
     2941 ccgcaaggtt tgcccaggcc cgcagcggag agcggcctgt atcttcacct gctcggtgaa
     3001 gcgttccgcg cgagtttcgt cgaccgaatg gctcacgtgg gagaccctgc gctggtgccc
     3061 gatcccgcac ctcgtttact ggacgcggct cacctgaatg agctccgtaa acggattgcc
     3121 atcgatcgaa cccactccat tccgctgctg aacaccgagg aacacggcac acatcatctc
     3181 gtcgtggcgg acgcacaagg catggtggtt tcgctcacga ccacggtaaa tcgaaccttt
     3241 ggagccaaga tcgtcacgcc cgactcgggg ttggtgctca acgatcaact cgacgatttt
     3301 gcgctctcgc gtgacgtcga ccatctgggc gccggttcga gggtcgcccc gaacgcgccg
     3361 agacccggcg cgcgcccagt atccagcatg actcccagta tcgtgctgag agcgggacgt
     3421 ccgatcctcg cgattggcgg ctctggcggt ctcaccatcg gaaccaacgt gacgcaactc
     3481 ttgatcgccc acctggcgct cggccaggcc ccgaaagaca tggtgcagcg cccgcgtata
     3541 tacgtgccga tgggcatcaa agaaacgttg ctcgtcgact cgggcttttc cgacgcggtg
     3601 atcgaggatc tcacgttccg cggtgaacga ctggcaccta tgaagttcac tggcagcgcg
     3661 gtccagctat tggcgtggga ttcgcgcggc tggtccgcgg ctgccgatcc acgtaagttc
     3721 ggggaagccg ctacgcaata acgcgggcct cgcgctcacg cgcggagccc caaggctacc
     3781 ttgagcccgg acgcgactga gcccagacgc gactgagccc ggacgcgact gagctcggaa
     3841 gctcagcgca gagcggcgac caactcggcc gtctccgcgt cgttcaagtc actggccagc
     3901 gcgtaaccaa cgcccgcgcg ttccgtcgcg ccgatccaat agccccggcg agccccgacg
     3961 tagaccggag cattggcgac caagcgcttt tccagtcgtt gttccagcgg tacccgcgac
     4021 gggtcataga cgtacaacgt gacccgatgc ccactcacct tgtaacgcag ggatgcggca
     4081 cgttgattgt tgtgaacggg cactaccgtg ccaccctcca acaaaccgcc gtattgcttc
     4141 aggctgggga tgcgcaccgg cacgcccacc tcacgctcga actgggcccc atcactcggc
     4201 aagagcgcga gatccgacgc ttggggcaca tcgtgcacgt gatgatcgat cagttgctca
     4261 atcacctggt ctgcattcac cacgctcgcg ttagcgtgac ttgcagtctc gttccgctcc
     4321 aggcgcctcg acgacggatc agaagcagcc cagaacagcg taaaccccgc cgccgcagcc
     4381 accggcaaga tcgtacgcca ggagagcatg cggttgctct cggcttggcg cgcgacgacg
     4441 tgtgcttccc actcgcgctc gcgcgccgcc tcgagcgcca gcttgagtcg ctcccgaaaa
     4501 gcatcgctcg gcaaagccaa tggctctgat acacgccgca agctggaacg aaatgcttgc
     4561 ccgagccgca cgcgctcggc acacactcga caaccctgca cgtgctgctc cacctctacg
     4621 accttctctg gagagagctc gccatccaca aacggctcca gccacggcac gacggtacgg
     4681 cagttcatgc gcgggctcct ttacgtttgc gataagtctc catctcgacc acgggctgct
     4741 ccgcgttcgc cgcgcccacc gccgcttccg ggcccacgat accgagcgcc aaagcgtgct
     4801 cgtagaggcg cgactttaga atgcgccgac cgcggtgcag gcgcgacatc acggttccaa
     4861 tggggcaacc catgatgtcc gaaatctcct tgtaggagag gtcttctatg tccgacagca
     4921 ctaccgccaa acggaaatcc gtcggcagct cctgcaaagc ctgatggatc tcagcttcga
     4981 ggattcgccg taaggcctgc gactctgggt cacgcagcgc ttccatcgtt gcggcgctca
     5041 cccagccgtc cgccaacgga tccgcgtccg gaccgtccaa cacgctgcgc tccaaaccgc
     5101 cacggcgata gcggttgatg aacgtattgg tgagaatctt cagtaaccag gctttgaggt
     5161 tcgtacccga ttcgaactga tcgcgagcac gaatcgcctt caacacgctg tcttggacca
     5221 ggtcctcggc ttccgagccg ttcctcatca gtcgattcgc gaccgctaat aaggagggca
     5281 gatgttcgag aacctcgcct tcgaactcct gacggtggga aacgcgggcc atcatagcgg
     5341 gatatcggtt caacccgacg ccctggcaac ctattcccgg ggttcagcgg gagccgcggg
     5401 aggctcttgg ccaggcagcc gacgctcgat cgcgtcgagt cgctccgaaa gacgccgaat
     5461 ttcctgctcg agatctctaa at
//
LOCUS       DASTKU010000880        11455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_172407, whole genome shotgun sequence.
ACCESSION   DASTKU010000880 DASTKU010000000
VERSION     DASTKU010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11455
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172407"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VFQ61_30970"
     CDS             complement(<1..779)
                     /locus_tag="VFQ61_30970"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9958968.1"
                     /translation="MTALINGHNGAPAPLELGRLLAGRHLVVVGGTGFLGKVWLSYLL
                     HHFPEIGQIHLVVRAKGDMSAQQRFEREIAPSAVFDPVRQTHGANYEAWLASKVVPVF
                     GDVVKPFCGLDVGLREELRGKIDAVVNVSGVVDFDPPLDEALEVNAFGVQNLVALARD
                     LGSVPLLHTSTCYVAGNRTGVVEEKHPYEHPFPRASELERSHWEPDREISECLDVIEQ
                     ARHRGNDAFRQSRFLDEAKKNLRDRSEPARGKVLENEVQKV"
     gene            complement(776..2383)
                     /locus_tag="VFQ61_30975"
     CDS             complement(776..2383)
                     /locus_tag="VFQ61_30975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HET9958969.1"
                     /translation="MNHPCLVTLERDSAPRILFSGDRLVEVDLPPGTRCIYPKPPLAP
                     IKDVDAAIRYAINHPEGSEPLYAKLRPGMKVTIAVDDISVPLPPMRRPDVRERLLKVV
                     LELLAEHAVDDIHIIVATGIHRRMKAHEIRHMVGDKVFNEYYPERLYNHDAEDEKNMV
                     LVGHNDHGHGLELNRRAVESDLLIYLNVNFVAMNGGYKSVAVGLCNYRSLKPHHNPIT
                     IRESDSYMDPKKSRLAHDIARMGKVAHKHLNVFTIETTVNNRMFDKNLDFLCKNEDEL
                     SSKERLLAKALIAATAKLPQPARQAIFDKFPAPYEVTGVFAGSGPAIHDRAIDKCFSQ
                     YLVPVEGQSDVLVMGIPFISPYNVHAFLNPLLVQVLAQGYLFNMYRGAPLVKRGGTLI
                     LTHPCTDKFDHEQHAPYIDFVHRLLPETRDARELESRYQDKFAQDPAFIQMFRDGYAY
                     HPAHPFFMWYWGENGRQYLGRVIVVGADNEYIPKLLGWETAPSMEEALYRAKNGEARS
                     LDITLLRVPPIVMGDVTVRPQAGGGSP"
     gene            complement(2380..3039)
                     /locus_tag="VFQ61_30980"
     CDS             complement(2380..3039)
                     /locus_tag="VFQ61_30980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET9958970.1"
                     /translation="MAACYFDVDGTLVATNLVHPTLFYLMNQGTPLQSALRLGKTLLG
                     APRMAIAELYDRRVFNELLFSAYAGMSEDRLELLGEEAFEKVMLPSLYASAHGLVQKS
                     LDAGHAVVFVSGALECVVRHLADHLGATHVIANRLEMKEGIATGKLLRPVVAGPEKAR
                     IVRDHARAQGFDLDDCFAFSDSYSDVPMLSVVGHPAAVNPDKRLALLAKAYSWPSFDL
                     R"
     gene            3226..4365
                     /locus_tag="VFQ61_30985"
     CDS             3226..4365
                     /locus_tag="VFQ61_30985"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958971.1"
                     /translation="MKLVEDPAGLAAACQALESAPVLYLDTEFDASRDGTRLCLVQIS
                     GGAEAYLVDTLKLSELGPLSALLGHPDRTWVLHAGQQDIQLLKLRLHAAPTRLFDTQV
                     AWALSSIEHSASLSYLKYHLLGLRGEKSHQADDWLRRPLPPAQLAYAAEDVHHLPALF
                     AALTQKLDRLSRTEACYAASRESVTPVEDVFEPLTLEAFRNAWQLEPEGQAVLRYLVT
                     WFNELSPDQRSSAPDFKGLFSLASRRPRTLDDLSRLRTLPRRASALFGRELVSGIARA
                     IAGAHTTSFQLLDPPPYAVPADLLAAGWLEAIRAELCIELTLAPELVLPARILRRLRE
                     AAVRQGQLEGSLAELQGWRADLLHAPLSAKMARYGSLKNLPSRLD"
     gene            4370..4759
                     /gene="apaG"
                     /locus_tag="VFQ61_30990"
     CDS             4370..4759
                     /gene="apaG"
                     /locus_tag="VFQ61_30990"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HET9958972.1"
                     /translation="MSSSEALTRGVRVHVEARFSPNHSDPKKKEWFFLYTITIKNEAR
                     ETVQLLNRHWTITDAHGHVEEVRGPGVVGKQPSLKSGESFEYTSGCPLKTPFGSMHGT
                     YEMLTEHGERFNATIAPFALREDEAFN"
     gene            4769..5866
                     /locus_tag="VFQ61_30995"
     CDS             4769..5866
                     /locus_tag="VFQ61_30995"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9958973.1"
                     /translation="MTEGQQPKLLPDSFVAPASEIRAAFAQASAAEVVFVARQCLETG
                     AWDQALAVCEALADRDDPVIEICAAVANFVAGERAVALHRIERVLAHRPDHLSALAVQ
                     AQITMRLGQPDRAVTSLLRLIDRYPDYPGAQPQLATLLMPGPHYRQVLTEIHERLRPK
                     TYLEIGFDTGATLSLARYSSCVVGVDPGSQEIKHPLPVGARLFREESDVFFAQHTRAE
                     VLGSRQIDLTFVDGLHRFENVLSDFANAEAWSHPGACIVLHDCVPLVRGTATRERRTR
                     FWVGDTWKIVPILRRFRPDLQITTLPCPPSGLVIVRALRPGSQPDWTRDRDAMLEAGA
                     TLEWTSEPGSIPAEFNAVTNDERGLMQALGL"
     gene            complement(6008..7918)
                     /locus_tag="VFQ61_31000"
     CDS             complement(6008..7918)
                     /locus_tag="VFQ61_31000"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HET9958974.1"
                     /translation="MRLRSVCAVILPLFGAITCSSDSSPDGVPGPESPAPKAGALIRI
                     STRSQVGVLLDEIPATQRDRVAQALLEKPESFFISRAKQQLTLATYRLNFRPAFYEED
                     EDKKQLPLPPDSVFEIQLLAGEDGKKVRRAEVEGHDYVLADYELKTVVVTDEQSPGIS
                     EPALAKVGGTWDEPFVFPVDPELLIQRTGYACMDEAEFPPNSVDSEDVEFFYDQTCEV
                     EEELTPDGCHYTELPEESCLDALTTHVGKVEAPLHYERIAYDAATAKKYRVGEVKTKG
                     AADLEVVTDELRVNRLTYRYIDEDSCALAEACVGGTGWRRLLQFNASEKNVGDAPIHI
                     GDVDYFVDDPENPTPNANHHVYQFSACHNHYHFNYFATFTYGNDPNLGSKRAFCLESV
                     ARYGNHESSPTWSPYNKCAYQGITQGWGDQYNAGIECQWVDVTTIDVSQGAVTKPLGL
                     RSNPEGFLCEGEPVVDAEGNPKWESTSLVTDDGEPVDRLMCDETDRWDSNNYGEHDVT
                     LPVAGEGMVTEPCTRGQIGPLRNCGFHYDGAIASCTAGKPASVRCTLEEGAEPQAIRL
                     CEASKVLSAGVACVESDALGSATLESGQSVSIEFKCPKERSRVEPGGLYSVYVAPVWP
                     EDELGKVTCTLE"
     gene            complement(8015..9793)
                     /locus_tag="VFQ61_31005"
     CDS             complement(8015..9793)
                     /locus_tag="VFQ61_31005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HET9958975.1"
                     /translation="MLARTSRFAFLKNGSRLLALLSLGAARSAFAQPIVAEPNGVMVP
                     QPPSPAEVSCCVTGRGYPASAASLSGLFAYRNDPNLDQYKDAKTGPGVFSPLCGFEGT
                     LVLRGGNCRLDFGWYNVTAGGAAPADNEIYTLIPKDDVDVYGNGSVDFCPLAGVPDAN
                     HQGEANKCKVKSFSAAAIRMDPHYKGGLIGFALRGDKNSRCSETHYSQNELHKSCTGA
                     TCPNGGHWIMSLQYESKAFPDSYYLAFEDKPVTTFGPNDEQNDGDFNDFVFFVTGVTC
                     AGGGQPCDASVGHPERKGVCAVGRTGCAAEGQMAACNPIAEASPEKCNNLDDDCDGAV
                     DNGQGLCEPGEVCDRGACVNACGSGEFRCPSDLVCSVTGYCVDASCKDVVCQDGQVCR
                     GGTCTGGCNGAVCPIGQECQLGRCIDPCDSIKCEDNQICEKGVCISSCTCRPCAQGKA
                     CRADGRCVDAGCESMTCDAASLCVAGQCKNACEGVVCPGGAACSNGKCGEPLTNYEPP
                     TNTGGSGGGVIPTNGGSPFVTGGMNSGNASNGTGANSSGTRRGSAETGGCNCHVASNR
                     GTSAGALAGLLAFASVLARRRRRTAA"
BASE COUNT         2140 a         3787 c         3418 g         2110 t
ORIGIN      
        1 ttgaccttct ggacctcgtt ctcgagcacc ttgccgcgcg ccggctcgct gcgatcgcgc
       61 aagttcttct tggcttcatc caagaagcgg ctctggcgaa aggcgtcgtt gccgcgatga
      121 cgagcttgct cgatcacatc gaggcactcc gagatctcgc gatcgggctc ccagtgcgag
      181 cgttcgagct cggaagcccg cgggaatggg tgctcgtacg ggtgcttctc ttcgaccacg
      241 cccgtgcggt tgccggccac gtaacaagtg ctggtgtgga gtagcggcac gctaccgaga
      301 tcacgcgcca acgcgacgag gttctgaacg ccgaaagcat tgacctcgag cgcctcgtct
      361 agcggcggat cgaaatccac gacgccggag acgttcacga ccgcgtcgat cttgccgcgc
      421 agctcctcgc gcagcccgac gtccaagccg cagaacggct tcaccacgtc gccgaacacc
      481 ggcacgacct tgctcgcgag ccacgcctcg taattggccc cgtgcgtctg ccgaaccggg
      541 tcgaacacgg cgctcggcgc gatctcgcgc tcgaaacgct gctgcgccga catgtcgccc
      601 ttggcgcgta cgaccaggtg gatttgcccg atctcgggga agtgatgcag caggtaggag
      661 agccagacct taccgaggaa cccggtcccg cccaccacga ccaggtgccg ccccgcgagc
      721 aaacgcccga gttcgagcgg cgcaggcgcg ccgttgtggc cgttgatgag ggcggtcaag
      781 gcgaacctcc gcccgcttgc ggacgcacgg tcacatcccc catcacgatg ggaggcacgc
      841 gcaacaacgt gatgtcgagt gatcgcgctt cgccgttctt cgcgcgatac agcgcctcct
      901 ccatgctcgg cgccgtctcc cacccgagca gcttcggaat gtactcgttg tcggcgccca
      961 ccacgatcac gcggcccaag tactgacgtc cgttctcgcc ccagtaccac atgaagaatg
     1021 ggtgcgccgg gtggtaagcg tagccgtcac ggaacatttg gatgaacgcc ggatcttggg
     1081 cgaacttgtc ctgatagcgc gactcgagtt cccgggcatc gcgcgtctcc ggcagcaagc
     1141 ggtgcacgaa gtcgatgtac ggcgcgtgct gctcgtgatc gaacttgtcc gtgcacgggt
     1201 gcgtgaggat caacgtgccg ccgcgcttca cgagcggcgc gcctcggtac atgttgaaca
     1261 ggtaaccctg cgcgagcacc tgcacgagca gcgggttcaa gaaggcgtgc acgttgtacg
     1321 ggctgatgaa ggggatgccc atcaccagca cgtcggactg cccttccacc ggtaccaagt
     1381 actgcgagaa gcacttgtcg atcgcccgat cgtggatggc ggggcccgag cccgcgaaca
     1441 cgcccgtgac ctcgtagggg gccgggaact tgtcgaagat ggcttggcgc gcgggctgcg
     1501 gcagtttggc cgtcgcggcg atcagcgcct tggcgagcag ccgctctttc gagctcagct
     1561 cgtcctcgtt cttgcacaag aagtcgaggt tcttgtcgaa catgcggttg ttcaccgtcg
     1621 tctcgatggt gaacacgttc aggtgcttgt gcgcgacctt gcccatgcgc gcgatgtcgt
     1681 gcgcgagccg cgacttcttc gggtccatgt agctgtcgga ctcgcgaatc gtgatcgggt
     1741 tgtggtgcgg cttgaggctc cggtaattgc agaggccaac ggccacggac ttgtagccgc
     1801 cgttcatggc cacgaagttc acgttcaagt agatgagcag atcgctctct accgcgcgcc
     1861 gattcagctc gaggccgtgg ccgtggtcgt tgtgcccgac cagcaccatg ttcttctcgt
     1921 cttcggcgtc gtggttgtag agccgctcgg ggtagtactc gttgaagacc ttgtccccga
     1981 ccatgtgccg gatctcgtgc gccttcatgc ggcgatggat gccggtcgcg acgatgatgt
     2041 ggatatcgtc caccgcgtgc tcggcgagca gctccagcac caccttgagc aaccgctcgc
     2101 gcacgtcggg tcgccgcatc ggcggcagcg gcaccgagat gtcgtcgaca gcgatcgtca
     2161 ccttcatgcc cggccgcagc ttcgcgtaca gcggctcgga gccctccggg tggttgatgg
     2221 cgtaacgaat cgccgcatcc acgtccttga tcggcgcgag cggtggcttc gggtagatgc
     2281 accgcgtgcc gggaggcaaa tccacctcca cgagtcggtc gccggagaac agaatacggg
     2341 gagcgctgtc gcgctcgagg gtgacgaggc aggggtggtt catcgcagat cgaagctcgg
     2401 ccagctgtag gccttcgcca aaagggccag tcgtttatcg gggttcacgg cagccggatg
     2461 acccacgacg ctgagcatcg ggacgtcgga atagctgtcg ctgaaagcaa agcaatcatc
     2521 taaatcgaaa ccttgcgccc gggcgtggtc gcgcacgatg cgggcctttt cggggccggc
     2581 caccacgggg cgcagcagct tgccggtcgc gatgccctcc ttcatctcga gccggttggc
     2641 gatcacgtgc gtcgcgccca ggtgatcggc cagatgccgc accacgcact cgagcgcgcc
     2701 ggacacgaag accaccgcgt ggcccgcgtc caagctcttt tggaccaagc cgtgcgcgga
     2761 agcataaaga gaaggcaaca tgaccttctc gaacgcttcc tcgcccagga gctcgagccg
     2821 gtcttcgctc atgccggcat aggcactgaa gagcagctcg ttgaacacgc gccggtcata
     2881 cagctcggcg atcgccatac gcggcgcgcc cagcaatgtc tttccgagac gcagcgcact
     2941 ctgcaaagga gtgccctggt tcatgagata aaagagggtt gggtgaacta agttcgtggc
     3001 aaccagggtg ccatcgacgt cgaaatagca agcggccacg ggaatcctcg ccctcgtaca
     3061 ggcaaacggg agcgtcaagc aacgccaaac gagggccccc cctttagcac acagaccggg
     3121 cgactgacca gaatgaactc atgggaagtc gctagggccg cgcggagcag cgcgctatga
     3181 gtgccgctcc gaatgccggc accacccacc gaagcaagcc cagccatgaa gttagtcgag
     3241 gatcccgcgg ggctcgccgc agcgtgccaa gccttggaat ccgccccagt tctgtacctg
     3301 gacaccgagt tcgacgcgag ccgggacggt acgcggctgt gcctcgtcca aatcagcggc
     3361 ggcgcggaag cctacctggt ggatacgctc aagctttccg agctcgggcc tctgagcgcg
     3421 ctactcgggc atccggaccg aacctgggtg ctgcacgcgg gccaacaaga catccagttg
     3481 ctcaagctgc gcctgcacgc ggccccgacc cgcttgttcg acacgcaggt ggcctgggcg
     3541 ctctcttcaa tcgagcattc ggcttccctt tcctacctga agtaccactt gctcgggctg
     3601 cgcggagaga agtcacacca ggctgacgat tggctacgtc gcccgttgcc gccggcccag
     3661 ctcgcctacg ccgcggagga tgtgcatcac ttaccggcgc tgtttgcggc tctaacccag
     3721 aagctagacc gcttgtcgcg aacggaggct tgttacgcag cttcccgtga aagcgtcacc
     3781 ccggttgaag acgtcttcga acccctgacg ctggaagctt tccgtaacgc gtggcaactc
     3841 gaacccgaag gccaggcggt gctgcggtac ctggtgacct ggttcaacga actctcgccc
     3901 gatcagcgct cgtcggcgcc ggacttcaaa ggcttgtttt cgttggccag ccgccgccca
     3961 cgcacgctcg acgatctctc tcgtctgcgc acgctgcccc gtcgcgcttc ggcgttgttc
     4021 gggcgggagc tcgtctccgg aatcgcgcgc gccatcgccg gcgcccacac caccagcttt
     4081 caacttctcg acccccctcc ctatgcggtc ccagcggatc tcctggcggc cggttggctc
     4141 gaagccattc gggcggagct ctgcatcgag ctcacgttgg ctccggaact cgtgctacct
     4201 gcccggatct tgcggcgctt gcgtgaggcg gccgtgcggc agggccagct cgagggaagc
     4261 ctggcggagc tccaaggctg gcgcgccgac ctcctgcacg ctccactttc cgccaagatg
     4321 gcgcgctatg gcagcctgaa aaacctgccg agcaggctag attagcgccg tgtccagctc
     4381 cgaagccctc acccgaggcg tccgcgtcca cgtcgaggcg cgattctcgc cgaaccactc
     4441 cgatccgaaa aagaaggagt ggttcttcct ctacaccatc acgatcaaga acgaagcgcg
     4501 cgagaccgtg cagctcctga accgtcattg gacgatcacg gacgcgcacg gtcacgtcga
     4561 agaagtgcgt ggccccggcg tagtcggcaa gcaaccgtcg ctgaagagcg gcgagagctt
     4621 cgaatacacc tcgggctgcc ccctcaagac gccattcgga tcgatgcacg gcacttacga
     4681 aatgctcact gaacacggcg agcgcttcaa cgccaccatc gcgccgttcg cgctgcgcga
     4741 agacgaagcc ttcaactaac agtgagccat gaccgaaggt cagcagccaa aactcctgcc
     4801 tgactcgttc gtggcgcccg catccgagat tcgggcggcg tttgcccagg cgagcgccgc
     4861 ggaggtcgtg ttcgtggcgc ggcaatgcct cgagacgggc gcctgggacc aagccctggc
     4921 cgtttgcgaa gcgctcgccg accgcgatga tccggtcatc gagatctgtg ccgcggtggc
     4981 gaacttcgtc gcgggcgagc gagccgttgc tctccatcgc atcgagcgcg tgctcgctca
     5041 tcgtcccgac catctctcgg cgttagccgt gcaggcgcaa atcacgatgc ggctcggcca
     5101 gccagatcga gccgtgacgt cactgttgcg attgatcgac cgctacccgg actatccggg
     5161 agcgcagccg cagctcgcca ccctgctcat gccggggcca cactatcgcc aggttctgac
     5221 cgaaatccac gagcggctcc gtcctaagac gtacctggag attggcttcg ataccggcgc
     5281 caccttgagc ctcgcccgat actcgagctg tgttgtcgga gtggatcccg ggtcgcagga
     5341 gatcaagcat cctcttcccg tcggagcgcg tttgtttcgc gaagaaagcg acgtgttctt
     5401 cgcccagcat actcgcgccg aggtgcttgg ctcacgtcag atcgacttga ccttcgtcga
     5461 cgggctgcat cgtttcgaga acgtgctctc cgactttgcg aacgcggaag cgtggtccca
     5521 tccgggcgcg tgcatcgtgc tgcacgactg cgtgccgctc gtgcgcggca cggccacccg
     5581 cgagcggcgc acgcgctttt gggtgggtga cacctggaag atcgtgccta ttttgaggcg
     5641 ttttcggccc gatttgcaga taaccacgct gccttgcccg ccttcggggt tggtgatcgt
     5701 gcgcgccttg aggcctggct cacagcccga ttggacccgc gatcgcgacg cgatgctcga
     5761 agcgggcgcg acgctggagt ggacctccga gcctggttcc attccggcgg agttcaacgc
     5821 ggtgacgaat gacgaacgcg gactcatgca agcactcggc ctttgagtga gcgcgaagag
     5881 cgcgttacga aaggcggcgg gcgcggtggg gcgacgggat gcagacgaat tgagtctgcg
     5941 cgagagccgt tagccgacga ctctcgcgct ctttacaggg cttgccccaa taaatacggc
     6001 cctgcggcta ctcgagggta caggtgactt tgccgagttc gtcctcgggc cacacggggg
     6061 cgacgtaaac ggagtacaag ccgccaggtt cgacgcgact ccgctccttc gggcacttga
     6121 actcgatgct gacgctctgc ccgctttcga gcgtggccga gcccagcgcg tccgattcca
     6181 cgcacgcgac gcccgcgctg agcactttgc tggcctcaca caaacgaatg gcctgcggct
     6241 ccgcgccttc ttcgagggtg cagcgcacgc tcgcgggctt acccgcggtg cagctcgcga
     6301 tggctccatc gtagtggaaa ccgcagttgc ggagtggccc aatctgaccg cgcgtgcagg
     6361 gctcggtcac catgccctcg cccgcgacgg gcaaggtcac gtcgtgttcg ccgtaattgt
     6421 tgctgtccca acgatcggtc tcgtcgcaca tcaagcgatc gacgggctcg ccgtcgtcgg
     6481 tcacgagtga agtgctctcc cacttcgggt tgccctcggc atccaccacc ggctcacctt
     6541 cgcacaggaa cccctctgga ttcgagcgca aacccagcgg tttggtgacc gcgccttgcg
     6601 aaacatcaat cgtagtgacg tctacccact ggcactcgat accggcgttg tactggtcac
     6661 cccaaccttg ggtgatgccc tgataagcgc acttgttgta aggcgaccag gtggggctgg
     6721 attcgtggtt tccgtagcgg gcgacgctct ctaggcaaaa cgcgcgcttc gagccgaggt
     6781 tcgggtcgtt gccgtaggtg aaagtcgcaa agtagttgaa gtgatagtgg ttgtgacagg
     6841 cgctgaattg atagacgtga tgattcgcgt tcggcgtggg gttctcgggg tcatcgacga
     6901 agtagtcgac atccccgatg tggatcggcg catcgcccac gttcttctcg ctggcattga
     6961 actgcagtag acgccgccaa ccggtaccgc ccacgcaggc ttccgcgagc gcacacgagt
     7021 cttcatcgat gtagcgataa gtcaggcggt ttacgcgcag ctcgtcggtg accacctcga
     7081 gatcggccgc gcccttggtc ttcacctcgc ccacgcggta cttcttggcg gtcgccgcgt
     7141 cgtaagcgat gcgctcgtag tgcagcggcg cctccacctt gccgacatgc gtggtgagcg
     7201 cgtccaaaca ggactcttcc ggcagctcgg tgtagtggca gccgtccggc gtgagttctt
     7261 cttcgacctc gcaggtctga tcgtaaaaga actcgacgtc ttcactgtcc acgctgttgg
     7321 gcgggaactc ggcctcatcc atacaggcgt aaccggtgcg ctgaatcagg agctcgggat
     7381 ccacgggaaa cacgaacggc tcatcccaag taccgcccac cttggccagc gccggctcgg
     7441 atattcccgg tgattgctcg tccgtaacca cgaccgtctt cagctcgtag tcggccagca
     7501 cgtaatcatg gccttcgacc tcggctcgcc gcaccttctt gccatcttca cccgcgagca
     7561 attgaatctc gaaaaccgaa tctggcggga gcggaagttg cttcttgtct tcgtcctctt
     7621 cgtagaatgc ggggcggaaa ttcagccgat aggtcgccaa cgtgagctgc tgcttggcgc
     7681 gtgagatgaa aaaactctcc ggcttctcga gcagcgcttg cgccacgcgg tcgcgctggg
     7741 tcgccgggat ctcgtcgagc agcacgccca cttggctgcg cgtcgagatc cgaatcagag
     7801 ccccggcttt gggcgcgggg ctctccggac cgggaacgcc atctggtgaa gaatcggaag
     7861 agcaggtgat ggccccaaac agcggcagaa tcaccgcgca aacgctgcgc aatctcatgc
     7921 tggtcaccct acgaagcgga gaggtcttgt ccgtttcgca tgagtaacca gcgagtgtca
     7981 atccgagtgg tcgattccgg ttgtgccgcg ccgctcaggc cgccgttcgg cgacgtcggc
     8041 gcgcaagcac gcttgcaaac gcgagcaggc ccgcgagcgc ccccgcagaa gtgccccgat
     8101 tggatgccac gtgacaattg cagccgccgg tctctgcgga gccgcgccgc gtacccgacg
     8161 agttcgcgcc cgtgccgttg ctcgcgttgc cgctgttcat gccgcccgtc acgaatgggg
     8221 agccgccatt cgtgggaatc acaccgccac cgcttcctcc ggtgttggtt ggaggctcgt
     8281 agttcgtcag cggctcaccg cacttgccat tcgagcacgc cgcgccgccg gggcacacca
     8341 ccccttcaca ggcgttcttg cattgacccg caacacacaa gctcgcggcg tcacaggtca
     8401 tgctctcgca acctgcgtcc acacagcgcc catccgcgcg acaagcctta ccttgcgcgc
     8461 aaggccgaca agtacaggac gagatacaca cgcctttttc gcagatctgg ttatcttcgc
     8521 acttaatcga atcacaagga tcgatgcagc gcccgagctg gcactcttgc ccaatcgggc
     8581 acaccgcgcc gttgcaacct ccggtacagg taccgccgcg acagacctgg ccatcttggc
     8641 agaccacgtc cttacaagac gcatccacgc aatacccagt cacgctgcac accaaatccg
     8701 atggacaacg gaactcaccg cttccgcaag cattcacgca cgcgccgcgg tcgcacacct
     8761 caccgggctc acacagcccc tgcccgttat ccaccgctcc atcgcaatca tcgtccaggt
     8821 tattgcactt ctctggcgaa gcctctgcaa tcggattgca cgcggccatt tgcccttctg
     8881 ccgcgcagcc cgtgcgaccc accgcgcaca cacctttgcg ctcggggtgc cccacgctcg
     8941 catcgcacgg ctggccgccg cccgcgcagg tcacgccggt cacgaagaag acgaagtcgt
     9001 tgaagtcgcc gtcgttctgc tcgtcattgg gcccgaacgt cgtcaccggc ttatcctcga
     9061 acgcgagata atacgagtct gggaaagcct tcgactcgta ttgcagcgac ataatccaat
     9121 gcccgccatt cggacacgtc gcccccgtac agctcttgtg cagctcgttt tgcgaatagt
     9181 gcgtctcact gcagcgactg ttcttgtcac cgcgcagcgc gaacccgatc agaccgccct
     9241 tgtagtgcgg atccatgcga atcgccgccg ccgagaaaga ctttacctta cacttattgg
     9301 cctcaccttg atgattcgcg tccggcaccc cggcgagcgg gcagaagtcc acgctaccgt
     9361 tgccgtacac atccacgtca tcttttggga tcagcgtata gatctcgtta tccgcgggcg
     9421 cggctccgcc cgccgtcacg ttgtaccagc caaaatccag acggcagttc ccaccgcgca
     9481 gcaccagagt cccctcgaac ccgcacagcg gcgagaacac ccccggtcct gtcttcgcat
     9541 ctttgtactg atccaagttc ggatcattgc gataggcgaa caagcccgag agcgacgccg
     9601 cgctcgcagg gtaacctcgc cccgtcacgc agcaactcac ctctgctggc gatggcggct
     9661 gcggcaccat cactccattc ggctccgcca caatcggctg cgcgaacgcg ctccgcgccg
     9721 cgcccagcga taggagcgcc aacagacgag acccattctt caagaaagca aagcgactgg
     9781 ttctggccag cataacgagg gaggctagca ggtcggaggc gggcgtcgaa aggcgtgctg
     9841 ggtgcacgtc agagcggcga acggaactgg gtcagattca gcaaaaggcg gggagaatgg
     9901 cccccaaccg gagtcaattc tgacgatcct tccgcagaga ttttgcggat cgtcgccgcg
     9961 cccgccctct acgccaattc cagacggcca tagtcccccc gaagccaacc gcgacgctca
    10021 gccagctgga ctctggttga cccccgaagg tgcacccgcc gccgctgccg tgaaccgggg
    10081 cacacacgtc ctcatcatca acgtgggtgc aactccatcc ggaggggcag ttgggaaaga
    10141 agttaccgca acgctgagtg caaacgggat gaggtcgcgc gttttcaagg caacgagccc
    10201 cgcggcaatc ggaggacgaa gcgcaccgct ccccgagctc cgctcctgat tcgaggcaga
    10261 cggtgtgaag cggatcaatg ctttggcaag tcctcggtaa cgagcaatca ctcgcaactt
    10321 ggcaggtgtc gtggcagcgc tcgttcacgc aaagcccctg ctggcagtcc gtggcggttt
    10381 gacagacctc gtctgcgagc cgttttccac gggcgcatct cctgtcttgg cagacctcga
    10441 actcggcgca ttcatcatcc accgagcacg attgaccgac gcatggtcca tcagcacaac
    10501 cttcgggctc ctcgcatcca tggagagtgc agaccaattc gttcgagcac agctcacccc
    10561 cacattcaat gcgacactca ccctcgaagc aagtctgccc tgttggacac ccttcttcag
    10621 cgcagtcgcc gcggggatac agatagcaga tcccctcgat gtcatctgct tccagcgtga
    10681 cttggccagg gctatatgct ggaaacatgg ttacgcttgc gtcgctctca tccgtgcaaa
    10741 ttggaacctg atctagttcg cacgggtgca gcaatcccaa agcatgtcca agctcgtgca
    10801 cgagcacagc tcgcagactg cgccgcgagg catcagcgcc aacctgtgcc gaccaatcca
    10861 ccgaaccgtt caaatagata tccgtctcga cgattgacca gtttccgtct gcggggcgcc
    10921 taaactgcac atcagtgaat ccggcggatg ttggctcgaa accccggttc ttccagtcag
    10981 taacgaactc gatcgaatta atgccgtccc cagccctggc gggttcgtca gagaatccgg
    11041 taaaactcaa agtaaccgca ctacatgaag gttctgccca ggagagcgcc gcagcccaga
    11101 acccggcctc caagtccaga tccgaaatgc tagccggtag gctacgcgtc gcctggaaag
    11161 cgaaaggctg cccctcaaat cgaacgggcg aggtggcttt cacctcggct agctcccgta
    11221 ccgtccgaaa cgcatatgca ggaagcgacg ccatcgcgac ggtcgcacca atcaatcctg
    11281 cgatagcaaa gtggcgcatt tgagagcaat ccttagttgg cgagaaacca gactcgagcc
    11341 gcgcttgctc ccacggcagt ccgctttcgt caagcgctct caggtgaagt ggcgacacca
    11401 cacaaattgc agacggactc aatcagagcc ccgttcgcaa ccgctcctcg gcgat
//
LOCUS       DASTKU010000881         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1065659, whole genome shotgun sequence.
ACCESSION   DASTKU010000881 DASTKU010000000
VERSION     DASTKU010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065659"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFQ61_31010"
     CDS             <1..274
                     /locus_tag="VFQ61_31010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958976.1"
                     /translation="TCRRRVVSIASELGDSYTEVPAGAGGKFRNECRDPDFVSRSAVP
                     GSRMTRDFFHRVAIWDSSIMQKRRACPARVRLRSLLDQREPGRVRS"
     gene            314..1960
                     /locus_tag="VFQ61_31015"
     CDS             314..1960
                     /locus_tag="VFQ61_31015"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET9958977.1"
                     /translation="MKASFPTLVLLGAASLIACSPAEEGGNGFGGKVGKGGAKGSGGT
                     NAGGSVSGGASSGGAAFGGTTSGGTSSGGASAGGTNSGGQSPGGTANGGSSSAASGGV
                     TALSGGAANAGSANGGAANGGTASGGSANGGTAQGGTSSGGAASGGTANGGMSNGGTA
                     QGGASSGGAMQGGTANGGASGKGGSGSGGAGSGGAASGGSAGNTAKGGTTGTGGTTSS
                     GGTGGTSTIGPSDLPVPAGPSNVPKPTQAAGGFKILDWAGFKGAISFTFDDALGSQLA
                     HYAEFKATGVRMTFYLVCNNDMSNTAWKTIAQDGQELGNHTNHHCNANGSGCGWGSFT
                     GTSSEFDDCTAHLKSAFGLKDVYTSASPMGDVGWDTPASTRFLANRGVQDTGGVAPND
                     NTNPYELPCHVADANEKAASGTRSFNGVTDNARANNRWRIILNHSVGNNDGYNPVAAS
                     EVVAAMTYAKGRGDVWVDTVANVAAYWRAQKLVSSATPQTAGTSTTWSWTLPAHFPPG
                     RYLRVTVNGGTLSQKGAPLTWDGHGYYEVSLDAGSVTLSP"
BASE COUNT          399 a          646 c          724 g          317 t
ORIGIN      
        1 gacgtgtagg cggcgagtcg tttcaattgc atcggagctc ggggactctt acaccgaggt
       61 ccccgcgggc gccggtggaa agttccgcaa cgagtgtcgc gaccccgact tcgtgtcgcg
      121 ctcagccgtt ccgggttcac gaatgacccg cgatttcttc catcgcgtag cgatttggga
      181 cagctcgatc atgcagaaaa ggcgagcgtg ccccgcccgc gtgaggttac gctccttgct
      241 cgaccagcgc gaaccgggtc gcgtgcgctc gtgaagcgcg cggcctgtcg cctcgagcgc
      301 gtacgagatc cacatgaagg catcgtttcc gaccctggtt ttgcttggag cagcttctct
      361 catcgcatgt tcaccggcgg aagagggtgg caacgggttt ggcggaaagg tcggaaaagg
      421 cggcgccaag gggtcgggcg gaaccaacgc tgggggctcg gtttcgggcg gcgcgtcctc
      481 tggtggtgct gctttcggag ggaccacatc aggcggaacg agttccggcg gggcctcggc
      541 aggaggcacg aattcgggcg ggcaatctcc gggtgggacc gcgaacggtg gctcgtccag
      601 cgcggcaagc ggtggagtca cagcgttgag cggcggtgca gcaaacgccg gaagcgcgaa
      661 tggcggcgcc gccaacggcg ggaccgcgag cggcggcagc gcaaacgggg gaacggctca
      721 gggcggcacg tccagcggcg gggcggcgag tggaggcacc gcgaacggag gcatgtccaa
      781 cgggggcacg gctcaaggcg gcgcgtcgag cggtggtgca atgcagggcg gcactgccaa
      841 tggcggtgct tccggtaaag gcggctctgg cagcggcgga gcgggctcag gcggggccgc
      901 gagcggcggc agcgccggca acacggcgaa gggaggtacc accggcacgg gcgggacaac
      961 gagctctggc ggcaccggtg gcacctcgac gattgggcca tcggaccttc cggtccccgc
     1021 aggcccgagc aacgtgccga aaccgaccca agccgcaggt ggcttcaaga tcctcgattg
     1081 ggctggcttc aagggagcga tttctttcac gttcgacgac gcgctcggct cgcagctcgc
     1141 gcattacgcc gagttcaaag cgactggcgt ccgaatgacc ttctacctcg tttgcaacaa
     1201 cgacatgagc aacacggcgt ggaagacgat cgcacaagat gggcaggagc tcggcaatca
     1261 cacgaaccat cattgcaacg cgaacggcag cggctgcggt tggggaagct tcaccggaac
     1321 gagctcggag ttcgatgact gcaccgcgca cttgaagtca gcgttcgggc tcaaagacgt
     1381 ctacaccagc gcctccccaa tgggcgacgt cggttgggac actccggcat cgacgcggtt
     1441 cctcgccaac cgcggagtgc aagacacggg aggagtcgct ccgaacgaca acaccaaccc
     1501 gtacgagctt ccctgccacg tggcagacgc aaacgagaag gcggcgtctg gaacgcgcag
     1561 cttcaacggc gtgaccgaca atgcgcgcgc caacaaccgc tggcgcatca tcctgaatca
     1621 ctcggtcggc aacaacgacg gttacaaccc cgtcgcggcg agcgaggtgg tcgcggcgat
     1681 gacgtacgcg aaaggtcgcg gcgacgtttg ggtcgatacg gtggcgaacg tcgcggccta
     1741 ctggcgcgcc caaaaactcg tctcgagcgc tactccccaa acggcgggca ccagcaccac
     1801 gtggtcgtgg accctaccgg cgcactttcc cccggggcgc tacctgcgcg tcaccgtcaa
     1861 cggtgggacg ttgtcgcaaa agggcgcccc gctcacttgg gacggccacg gctattacga
     1921 agtgtcgctg gacgcgggct ccgtcaccct ttccccttag acccggcggc acggcaaaca
     1981 cgccccactg agtcattttg ccccaaccac gaaggggcat tttgaactct ggggcgaagt
     2041 cgcgagtgat aagttcgaag ggtcatggga atgagccttc gggtgc
//
LOCUS       DASTKU010000882        18539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1513936, whole genome shotgun sequence.
ACCESSION   DASTKU010000882 DASTKU010000000
VERSION     DASTKU010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 18539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18539
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513936"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VFQ61_31020"
     CDS             <1..1161
                     /locus_tag="VFQ61_31020"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HET9958978.1"
                     /translation="HGWVADQLQRLRGSLKDCQILELYAGAAAMGLALAARGARVTAV
                     DQYAPSLAQAKRAASAQGLSLDTRAEPAEQALSLVQTQNYEAVIVDPPRRGLSPAVRE
                     AMGGAAARWFVYVSCEPTTLARDLAHLARLGLRVEQLAPLDMIPHSESVETAVLLVRG
                     APVPPEVLYRDGCLLAIDKPAFLSLHHRQGGQPSLLDRVRSLDGFETAVPLDSEPLDT
                     AASGVCWFLARPELLTQFSHAWQARDEHWSVLAQGVLRGKGRLGPFTYRRERVVGTHS
                     LLSVSCLHEAADARSASGQAPRARRERTRPIGSYLKPIRHPLLGDERFGNPAANRYFI
                     ERHGLDRPFLHRRLVSWRPYGEAALEVSCEIPAELRQVLESLSAETGAAPGD"
     gene            complement(1197..1751)
                     /locus_tag="VFQ61_31025"
     CDS             complement(1197..1751)
                     /locus_tag="VFQ61_31025"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9958979.1"
                     /translation="MRTIPPWQVLSETVLLARRWLSVRKQQVQLPNGHLIDEYHLLDG
                     PDWAGVIALTPNQDVIFVRQYRHGIGRTSLELPAGIIEPKESKLDGARREFMEETGYF
                     AEDWQPLFSTSSEPARNSTWAHFFTARNAHLAGEAKLDPSEMLSVELHPANQIEQLIR
                     SGEICHGLHIAALLLAERNGFFAR"
     gene            1985..3532
                     /locus_tag="VFQ61_31030"
     CDS             1985..3532
                     /locus_tag="VFQ61_31030"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET9958980.1"
                     /translation="MWPELLARLEPLEDEEALRVIRLLARCPGMAEAVLEQLSTAAGS
                     SASISAKLRARWFELLARLAAREPSDRLRAALIVGLSDEHARCRRFAASALGELDDPA
                     AEDALVRALENAAPDLSKAVVDALGKLGSDSALHALSTPRLQAQASTLPDLARRVQRA
                     RLLIDRRTQRAVAGRVSLDTPLGFKARCVAFCRRGLVGHLLEGLSFANPRTRSDVSVE
                     FNHAGTLRELYRCRTALEFGLTIQVADAASDPATRIARTLSAAELLRGMRAWTDGAPR
                     FRLDWSDGGHQRALTYRVLEELHRLQVPLENDPRAACWTVRAPVSGRGELHLVPLPER
                     DPRFEYRVADVPAASHPTLAAALAQSGGQRSDDIVWDPFLGSGLELVERARLGSYARL
                     IGTDLSSRALQAARANLQSAGVQRYELHTSDALEFQPDDGVTLIVSNPPMGRRVARDG
                     SLNELLPRFVAHAARVLRPKGRLVWLSPLPQLTAQAAQSLPLRLESGPEIDLGGFSAT
                     LQIFRRV"
     gene            3616..5073
                     /locus_tag="VFQ61_31035"
     CDS             3616..5073
                     /locus_tag="VFQ61_31035"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF015406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HET9958981.1"
                     /translation="MRTLLWFRGKDLRVSDHPALASALAAGEIVPVFVLDPYFFAPAR
                     AAELPHRIQFLLGSLQSLAANLKHLGTELLLVSGKSVDVIPRLAARLKVNRIAAQRWT
                     EPFARERDRRVAAAAGVPFELFEGETLATPGTLRTNGGTPFAVYSAFARTFERRVRIP
                     APLPAPQSLPPIPANLRALVSQLAEPLPTPESLGLSVNPRLPDPGERAAKQRLQCFLE
                     NALASYAGDRDRLDLPGTSRLSQDLKFGTLSVRSAYRAASHSVEDAPTTSDGIARFRA
                     ELLWREFAYYLLWEHPELLESPFRREFAKFPYRSDEASWLAWVEGKTGYPVVDAASRQ
                     LLGEGFVHNRARMISASFLTKHLLIDYRQGEAHYMKYLTDGDWASNNLGWQWSAGCGC
                     DAQPYFRVFNPVTQGRKFDPMGDYVRRWVPELSALPNRYLHAPWEAPESVLKAAGVRL
                     GKEYPRPIVQHELARARFLDLASKTFKKSAPPRTG"
     gene            5241..5876
                     /locus_tag="VFQ61_31040"
     CDS             5241..5876
                     /locus_tag="VFQ61_31040"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9958982.1"
                     /translation="MKLYVNPLSPNCRRVLAVASYLGLKPDIRIVDFGKGEHRSPEFL
                     KLNPNGAVPLLEDGKLLLSESRAILHHLAAKKPEARLVPTDPVELVEVLRWQFWDAAH
                     FSPPNATLYFQRILQPMIGGDPDESRIRDAESQVVRYAKVLDHHLADRPWLVGRSITV
                     ADFTIAATLMHSGSAGIRLNEFSNVKGWYDHIAELDAWQATEPSAIQASKS"
     gene            6040..7551
                     /locus_tag="VFQ61_31045"
     CDS             6040..7551
                     /locus_tag="VFQ61_31045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9958983.1"
                     /translation="MKSLSIPRAPLLVDSMGTRERDGAAKSAPNHETSLGLAWIVRLR
                     WGTLGGQVAALAVAHFALAIQLPILALLPSMLVLALSNFALMHTLGQRTFPSHFIGAL
                     LLIDVCLLTASLSFTGGASNPFSAFFVVYVALAALVLGRDWAMTLVAFTSLCFGALFW
                     VPAAWLGPHAHHVATPQHLQGMWTAYALSACFVAYFVSKIANALRERDRRIFDLKRYA
                     AATERLASLSTMAAGAAHELGTPLATIAVASSELADGLRANADPASLLSEAHSIRKEV
                     NRCRELLDRLAAGAGVGAGERLEFHALSEVLENLHKELGTERAKRLVLTCRAPAQTRL
                     RVPLRALTQVLVNLVDNGLYAQRIGGNDAAIELELDLRTGQSSASQLGFRVLDRGVGL
                     SSSELGRIGEPFFTTKPQGEGMGLGLYLVRRLAQDLEGDFSIGARAGGGTEALLALPL
                     DPVNARRETEQMAGTSTLNPSVLESSMARRECGARAAPPRVSVPESRRAARDV"
     gene            7544..8080
                     /locus_tag="VFQ61_31050"
     CDS             7544..8080
                     /locus_tag="VFQ61_31050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9958984.1"
                     /translation="MSELNSSENSVLLVDDDLEFRSTLARAFRRRGWEVFEADSPAGA
                     LELARNESPEHAVVDLRMPEGDGIALVRALRALDPALRIVLLTGFGSIATAVECMRAG
                     AINYLTKPADVADILLAFEGQQQNPEIVVPSLDRVEWEHIQRVLHSCGGNVTQAAQLL
                     GLHRRSLQRKLRRNPRAR"
     gene            complement(8108..9697)
                     /locus_tag="VFQ61_31055"
     CDS             complement(8108..9697)
                     /locus_tag="VFQ61_31055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958985.1"
                     /translation="MSDPERTQNTPSSDVDAAERVERDGAAISDPEPVIASQLNPISR
                     AELQSPVSGPVSEPGARSGARRSREERSSASPFSPLQPFEPGLMECGAELMHEKTSIS
                     HRRSSIKPAPADWRPQSSRRPAAPMVVVRSGDAQPLSPGAATDSSAADPLDWRAAGFR
                     EPFPLARDLQVRGAHAAQPQASPALRREEMASRHGDHTAQHWDDRAQPDSAVASSGRL
                     GKRGDSEQASERARAAFRDAASQSSGRMAARAAERTQAGPPDRAAINPAEGVHLASTD
                     GDGAVTESGPRERVLPAANSASIESKPRRGRQIVLAAGLALASSVIAFTLVRLAWPPR
                     ELPRHPKTQAATPAADARSRGNSDAQGLALGMDRASLVGSAVVEAATAALPPTAADSA
                     IATSTETTASGSDSSADATDSPAAASASAESNGDTANPNTDANPNTESNPGAPEATSA
                     ATLKEAGAAKRRVRLDVVPFDAKVSLHGLTQKGPPYFFDVPNGKHVAVEVVRRGYVTR
                     RVEIDGARPTLVIGLLKKPGR"
     gene            complement(10229..11968)
                     /locus_tag="VFQ61_31060"
     CDS             complement(10229..11968)
                     /locus_tag="VFQ61_31060"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9958986.1"
                     /translation="MTNRKHLVRELPPYAVRRGVKPALSALVLAFVSAQLFSSEAQAQ
                     GAPARPAPGARAPGATPPAATPPAGTQPAGTPPAATPPPATLTAPGARAPIGATPGAA
                     APATGAAAPAPATEPNDEAAAVLVAQPGGLTAQHVAERSAETSQSVRVRLAELDSARA
                     KLDQTTAQFFPRLTLRASYTRLSKVKSQIGPGGVVGAQANGGVFVDPNCQLERCPVVD
                     SEGTPIGAQSLAFPALQNNYALTASLSVPISDYVLRLSTATSGAAATERASQLNAKAE
                     RLKVQTDARAVFYDWLRAKARVRISEKSLDRVRARLKDARVAFELGTITRADVMRLEA
                     QAAATEQVVLESRAYQDMDEVQLAVFLGDAEPRTYENGEDISSLPAERRDTLAALTRE
                     AFSNRIELQALGEASEAARLASKAVQRAGYPRVDATGDVTYANPNQRYFPQRAEWKST
                     WSVGIAASWTIGDVFVNNASARDLAATSRSLEAQRAQLRDAIRQEVANQYLALTRARG
                     ALESARRGLAAAQEAYRVASDLYRVGKATTTEVIDAETELLSARISELNAGIDLRIAD
                     LRLTHAAGRDVPR"
     gene            12303..12707
                     /locus_tag="VFQ61_31065"
     CDS             12303..12707
                     /locus_tag="VFQ61_31065"
                     /inference="COORDINATES: protein motif:HMM:NF021758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HET9958987.1"
                     /translation="MPRDPRVEIQYCPRCRWLLRAGWLAQELLTTFEGQLGEVALVPG
                     ASGEFDIYLGHELLFSRRARGRFPEAKELKQLIRDRIAPDRSLGHSDRKTEPLAEQSP
                     ESKMSDRAPAAEPAAHEAGEPGNEAGDYCPTD"
     gene            12922..13737
                     /locus_tag="VFQ61_31070"
     CDS             12922..13737
                     /locus_tag="VFQ61_31070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958988.1"
                     /translation="MNSAFPSRGTPGEAHASATAAPSLPHVPSYSPTRLPDGASPSPA
                     PSSSALSSSAPSPYTSSPNDEGTIRDHSEATAAPIWQRSERDQAETLARESEFEWRPW
                     LTRVASGFGLAAIYGAALGSRAGGFSLLRHAVAVPLGLLGVSAVGLFALLVSLSLLDA
                     QSSPGQILSAAARSISSTGLILAGIAPATALLVVTIREAGAAQAVAAGGLLLAAAVGI
                     LPALKVTYRALSPEAWPFTLRAPLFVTGFAVFSFALGARLFFSLLPVLAGGAS"
     gene            13734..14435
                     /locus_tag="VFQ61_31075"
     CDS             13734..14435
                     /locus_tag="VFQ61_31075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958989.1"
                     /translation="MSSTLATLDQLLRSPNRAAEQSRGGAQLQNLALASLGALLFGAG
                     LFGAVLASSRGGLQLLYSGLKLPLAILGTLIVSVPALYALARSLARPLPLAGAVSLAL
                     SAAARGALVLSALAPVAWLAIDRGLGYHGCVLLAAICYAIAGVSALRVLFSGVGGGLR
                     GAVLLALCSIVVLPAGGQTAWMLRPFVGRPAQAHVPFLRHKEGSFADSLLQSGRSSVG
                     LYRTMSSEEAEEFER"
     gene            14432..15121
                     /locus_tag="VFQ61_31080"
     CDS             14432..15121
                     /locus_tag="VFQ61_31080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958990.1"
                     /translation="MTLTDLGILYGLSGVMSAIWLRRRPVVGVRPSLGSTLLVVLVWP
                     LWVPVVLSAERPNPVVTQRTSLEMESALVEGHQAIQGTPLEPLLPRAALERMQTEVRR
                     AAERLAELDTLLSSPAFDRTQALEHLERLERRGASGRTLASARSHCESIERLAALRDR
                     DRAILEEVSELSRALRTQLVLSRYRGSSPSDAGDIVSELWARLEVLDIDSAPLARPPD
                     NDAREANPGRG"
     gene            15087..15809
                     /locus_tag="VFQ61_31085"
     CDS             15087..15809
                     /locus_tag="VFQ61_31085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9958991.1"
                     /translation="MTREKLILVVDDEKRIRDVVEYALGKDGFRVVCAAHGTDALTAV
                     EREAPDLIVLDVMLPGLDGIELCRRLRTRSNVPILFLSARSDEVDRVVGLEVGGDDYL
                     TKPFSPRELVARVRAVLRRFSTGQSPNEIASSAADPGARSTRISHGSIDLDPSGWTVR
                     CGSQSIALTATEFMVLAALLERPGVVLTRQQLMQRTHVSGVNVTERTLDTHVRRIRAK
                     FRPFGVDPITTVHGVGYKATPA"
     gene            15813..17363
                     /locus_tag="VFQ61_31090"
     CDS             15813..17363
                     /locus_tag="VFQ61_31090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9958992.1"
                     /translation="MVWLTTKFRARLARVLREFGRIRYRLLAVNLFIVLVPAAGLEFA
                     RVYERQLLEGLERDMENQASLVRVALELRGSLDLENKVELKELEQWLERAARGTRTRV
                     RVVHASLGVVLDSHSHGPPEGPRFDPPRLTAFAHSRLASEVARASSFPTSIQERSELR
                     AAFSGKRATATRVARKPPAVYLFLADPILREGKVWAAVYVTRSTEPVLVELHRIRRGL
                     LIVLAVALAIGAGTTLLLAWTISRPLERLASAARRIASGAVEVDLPSSGGGEISALSR
                     AFGEMTRKLHERHRYISEFAADVAHGFKSPLTSIRGAAELLNEGAAQEPEARDRFLGN
                     IVQDAERLDRLVSRLLQLSRIDSADTLPAWIELDALVKRVLRRFDRADAPVHLLQNSS
                     IPVLYAREGDLEIALGNLLDNAQRHAPPGSPIELRVSGRPSDGKVTFSVRDTGAGIAP
                     EHLPHIFDRFFTTDSEGQGTGLGLSIVKSVALAHGGQISVFSVPGQGARFDLCIPVAP
                     SNLVGLRS"
     gene            complement(17371..18210)
                     /gene="dapF"
                     /locus_tag="VFQ61_31095"
     CDS             complement(17371..18210)
                     /gene="dapF"
                     /locus_tag="VFQ61_31095"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET9958993.1"
                     /translation="MKLWKAHGLGNDYLVLETADALSPERVRDVCDRHSGVGGDGILE
                     PFDAGAHADFGVRIWNPDGSIAEKSGNGLRIFARWLLKERGAPSVFSLWTGTCKVGCR
                     ADRGQISVEMGLPSFAPSDVPIRSENEWIERAITLQGESVVATAVGMGNPHCVVFREE
                     ALDGLPWRRWGAELERHELFPNRTNVQIARVISEHEVEIRIHERGAGETQASGSSSCG
                     VVAAGVRTGRLRAGRVRVSMPGGELFVTSSERGLLLEGPVEPIGRIEVDSDWLTRRRM
                     SEG"
     gene            18304..>18539
                     /locus_tag="VFQ61_31100"
     CDS             18304..>18539
                     /locus_tag="VFQ61_31100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958994.1"
                     /translation="MSELPFAYASPRAPQSMGVYVHFPYCLQKCPYCDFLSVPVERPH
                     IPQQRYTDAVLAELEARRAELPPLPIHSIFFGGGT"
BASE COUNT         3048 a         6096 c         5919 g         3476 t
ORIGIN      
        1 catggctggg tcgccgatca gctccagcgc ctgagaggct ctctgaagga ctgtcagatt
       61 ctggaattgt acgcgggcgc ggccgctatg gggctggcat tggccgcccg gggggcacgt
      121 gtcacagccg tcgatcaata cgctccgagc ttggctcagg ccaagcgtgc cgcctccgcc
      181 cagggactct cgctcgacac ccgggctgaa cctgcagagc aggcgttatc tctcgtgcag
      241 acccaaaact acgaagcggt gatcgtcgat ccgccaagac gtgggctctc gccggccgta
      301 cgtgaagcca tgggcggcgc ggcagcgcgt tggttcgtct atgtctcctg cgagcccacc
      361 acattggcgc gtgatctcgc tcacctcgct cggctcggct tgcgcgtcga gcaacttgcg
      421 ccgctcgaca tgattccgca ctccgagtca gtcgagacgg ccgtgctgct cgtgcgcggg
      481 gcgcccgtgc ccccggaggt tctgtaccgt gacgggtgct tgctcgcgat cgacaagcca
      541 gcttttctct cgctccatca ccggcaaggc ggccaaccat cactcctcga ccgagttcga
      601 tcgctggatg gcttcgagac cgccgtacca ctggattccg aaccgctcga tacggccgcg
      661 agcggcgtgt gttggttcct ggcgcgcccc gaattgctaa cccagttcag ccacgcctgg
      721 caggcccgag acgaacattg gtcggtgctg gcgcaaggcg ttctacgggg caaagggcgg
      781 cttggtccgt tcacctaccg acgagaacga gtggtaggaa cgcactctct tctctcggtc
      841 tcctgcctgc acgaagccgc tgatgcacgc tcggcatccg ggcaggcccc gcgagcccga
      901 cgcgaacgaa cgcgaccaat cggctcctat ttgaagccaa tccgccatcc gctgctcggg
      961 gatgagcgtt tcggcaaccc agccgccaac cgctatttca tcgagcggca cggcttggat
     1021 cggcccttct tacaccgtcg tctcgtcagt tggcggccgt acggcgaagc cgcactcgaa
     1081 gtcagctgcg agatcccggc cgagctcagg caagtgctgg agagcctgag cgcggagacg
     1141 ggcgccgcac cgggtgattg actcgcgcgg cggccttcac tcgtccccgc gcggcctcaa
     1201 cgtgcgaaga agccgtttcg ctcggccaac agcagcgcgg cgatgtgcaa gccatggcag
     1261 atctctccac tccgaatcag ctgctcgatc tggtttgcag ggtgcagctc cacgctgagc
     1321 atctcgctgg gatccagctt ggcttcgccg gcgagatgcg cattgcgtgc agtgaaaaag
     1381 tgagcccatg tcgaattgcg ggcgggctct gagctcgtgc tgaacaacgg ctgccagtcc
     1441 tcggcgaagt agccagtctc ctccatgaac tcgcgccgcg caccgtccaa tttgctttcc
     1501 ttcggctcga tgatgcccgc cggcaactcg aggctcgtgc ggccgatccc gtgacggtac
     1561 tggcgcacga agatcacgtc ttggttcgga gtgagcgcga tcacgccggc ccagtcgggg
     1621 ccgtccagca gatgatactc gtcgatcaga tgcccgttcg gtagttggac ctgctgcttt
     1681 cgcacgctca accagcgccg ggccaagaga accgtctccg ataggacctg ccaaggcggg
     1741 atagtgcgca tggccgagcc gtttagcatg cctaggagcg ccgaggaggc cggggatcac
     1801 atcgggactc gagagttttg gcaaaaccga gcgtctctcc gcttctgaag tagagtggcc
     1861 gccgaccttg cccgcctcca attcatcctc tgattctggg tcctccgatc gattcgacgc
     1921 ccactctgca tccgaaccgc tcctccgatg cctgaacgat ccgggataca gccccccccg
     1981 gcgcgtgtgg cccgagctgc tggctcgact ggagcccctg gaggacgagg aagccctgcg
     2041 tgtgattcgg ctcctggcgc gctgtcctgg tatggccgaa gcggtgctgg aacagctctc
     2101 gacggcggcc ggatcgagtg cctctatcag cgcgaagctc cgcgcccgct ggttcgagtt
     2161 gctggcccgg ctcgccgcgc gtgagccttc ggacaggctg cgagcggcat tgattgtggg
     2221 attgtcggac gagcacgcac gctgccgccg ttttgccgcc agtgcattgg gtgaactcga
     2281 cgatccggcg gcggaggacg cgctggttcg agcgctcgag aacgccgccc ccgatctcag
     2341 caaggccgtt gtggatgcgc tcggaaagct cggaagtgac tcggcgctgc acgccctgag
     2401 caccccacgc ttgcaagcgc aagcttcgac tttgcccgat ctcgctcgcc gggtgcaacg
     2461 cgcgcgcctg ctgatcgatc gacgcaccca acgtgccgtc gcaggccgtg tgtccctaga
     2521 cacgccgctc ggattcaaag cgaggtgcgt ggctttttgc cgccgcggct tggtgggcca
     2581 tctgctcgaa ggactatcgt tcgcgaaccc gcgcacgcgc agcgatgtgt cggtcgagtt
     2641 caaccacgcg ggtactttac gcgagctgta tcgctgccgg accgcgctcg agtttgggct
     2701 cacgattcaa gtcgccgacg cggcctcgga tcctgcgacg cgcatcgcgc gcaccctgag
     2761 cgccgcggag ctgctgcgcg gcatgcgagc ttggacggac ggagccccgc gctttcggct
     2821 agattggtcc gacggcggac atcaacgtgc gctcacttac cgggtgctcg aggagctgca
     2881 tcgattacag gtgccgctcg agaacgatcc tcgcgccgcc tgttggaccg ttcgagcacc
     2941 ggtcagcggc cggggcgaac tgcatctcgt ccctctaccg gaacgggatc cacgcttcga
     3001 ataccgcgta gcggatgtgc ccgccgcctc tcatcccact ctagcggccg ccctggccca
     3061 aagcggagga cagcgttcgg acgacatcgt ttgggatcca ttcctaggta gtggtctcga
     3121 gctggttgaa cgggcgcggc tcgggagcta tgcgcgcctg atcggcactg acttgtcctc
     3181 gagagcgctt caagcggcgc gtgcgaatct gcagtcggcc ggtgttcaac gctacgagct
     3241 gcatacgtca gatgcgctcg agttccagcc cgatgacggc gtgaccctga tcgtgagtaa
     3301 tccgccaatg ggccgccgcg tcgcacgcga cggctcgctc aatgagctcc taccgcgctt
     3361 cgtcgcgcac gcagcacgag ttttgcgccc gaagggccgc ttggtgtggc tatctcccct
     3421 accccagctg accgcgcaag ctgcgcaatc tctgcctctc cgcttggaat ccgggccgga
     3481 aatcgatttg ggaggcttca gcgccacact ccaaatcttt cggcgtgtct aacttccggt
     3541 cgtaaacagc gggcaccata gccccggagc accgcgcggc ggcggccgtc gcgaccaact
     3601 gcgcgaattt ctgcaatgcg tactctgctc tggttccgag gcaaggactt gcgtgtatcc
     3661 gatcacccgg ctttggcgag cgccttggcc gcgggagaaa tcgttcccgt cttcgtattg
     3721 gacccctact tctttgcgcc tgcgcgggcg gctgagctac cgcaccgcat ccaattcttg
     3781 cttggttcgt tgcagtcgct ggctgcgaac ttgaaacatc tggggacgga gctcctgctc
     3841 gtttcgggca agagtgtcga tgtaatcccc cgcctggcgg cgcggctgaa ggtcaatcgc
     3901 attgcggcgc aacgctggac cgagcctttc gcccgggagc gggaccggcg tgttgcggcc
     3961 gcggcgggag tacccttcga gctgttcgag ggcgagacct tggcgacgcc gggtacgctc
     4021 cgtacgaacg gcggcacacc cttcgcagtg tattcagcgt ttgcacgcac cttcgaacga
     4081 cgggtgcgaa ttcccgcgcc cctccccgca ccccaatcgc tgccgcccat tcccgcgaac
     4141 ctgcgcgcgc tcgtatcgca attggcggag ccgctgccaa ccccagagtc tttggggctg
     4201 tccgtcaatc cgcgcttgcc cgacccgggt gaacgcgcgg ccaagcagcg gctgcaatgt
     4261 ttcttggaga acgccctcgc gagctacgcc ggagaccgag atcgcctcga tctcccgggt
     4321 acgagccggc tctctcaaga tttgaagttt ggtaccctat cggtacgcag cgcctatcga
     4381 gccgcgagcc actccgtcga ggacgcgccc accacttcgg acggcatcgc gcgtttccga
     4441 gccgagctgc tgtggcgcga gtttgcatac tacctgctct gggaacatcc cgagctcctc
     4501 gagtcgccgt tccggcgtga gttcgcgaag ttcccgtacc gctccgacga agcgagttgg
     4561 ctggcgtggg tggaaggaaa aacgggctat ccagtcgtcg atgcggcaag ccggcaattg
     4621 ctgggcgaag gcttcgttca caatcgagcc cgcatgatct cggcgagctt cctgaccaag
     4681 cacctcttga tcgactatcg ccaaggcgag gcccactaca tgaagtatct gaccgacggg
     4741 gattgggcct cgaacaactt gggctggcag tggtccgcag gatgcggctg cgacgctcag
     4801 ccgtactttc gcgtcttcaa cccggtgacc cagggccgta agttcgatcc gatgggagac
     4861 tacgtccgac gctgggtccc agagctgagt gctctgccca accgctatct gcacgccccg
     4921 tgggaagcac cagaaagcgt gttgaaggcc gcaggggtga ggctcggcaa agagtacccg
     4981 cggcctatcg ttcagcacga actcgcgcgg gcccgctttc tcgatttggc ttcgaaaaca
     5041 ttcaagaagt ccgctccgcc tcggaccggc tagaaaatct tcttgccaca acgttttgtt
     5101 gcggaaagag ggtgtccgcc cccgctcggc ctgttgtaga ttgcgtcgtc gcgccggtcg
     5161 ggcgcgcctt caccatcgag ccgaggccgc ttcgcccaat catcgggccg ggcgggccac
     5221 tggctccgag gacgagagcc atgaaattgt atgtgaatcc gctctcgccg aattgtcggc
     5281 gggtcttagc cgttgcttcc tatctgggcc tcaagcccga cattcgaatc gtcgatttcg
     5341 gcaaagggga gcatcgaagc cccgagtttc tcaagctgaa cccgaacggt gcggtgccgc
     5401 tgctcgaaga cgggaagctc ttgctctccg agtcacgcgc gatcctgcat catttggcag
     5461 caaagaagcc ggaagcacgc ctcgttccta ccgatcccgt ggagctcgtc gaggtgctgc
     5521 gctggcagtt ctgggacgcg gcccatttca gcccaccaaa tgcgaccctg tattttcagc
     5581 gcatccttca acccatgatc ggcggagatc cggacgagag ccggattcga gacgccgagt
     5641 ctcaggtggt ccgctacgcg aaagtgctgg atcaccatct agccgaccga ccctggctcg
     5701 tcgggcggag catcaccgtc gcggacttca ccatcgcggc cactctgatg cactccggtt
     5761 cggctggcat tcggctcaac gagttcagca acgtaaaggg ttggtacgat cacatcgctg
     5821 agctcgacgc ctggcaggca accgagccga gcgcgattca ggcgagcaag tcgtaggcag
     5881 ttcacgagtt cgttttggcg ccgcaggaag cttctgctgc gccaaaacga ggacggttcg
     5941 tctagcttcg cgcaattgcg ctcgagccgt gcgggtgcga cgttttgccg ctgtcatggc
     6001 gtgcacatcg aggctaaagc acgcttcgcc gttgcaaaca tgaagtcgct atccattccc
     6061 cgcgcgccgc ttctggtgga ctcgatgggt acgcgcgagc gggatggagc cgcgaagagc
     6121 gcgccgaatc acgaaacgag ccttggattg gcgtggatcg tccggttgcg ttggggcacc
     6181 ttgggtgggc aggtcgcggc gctcgcggtc gcgcacttcg cgctcgcgat ccagctcccc
     6241 attctcgcac tcttgccgag catgctggtg ctggcgctct ccaacttcgc gctgatgcac
     6301 acgcttgggc agcgcacgtt cccgagccac ttcatcggcg cgcttctgct gattgacgtg
     6361 tgcctgctta cggcatccct gtcgttcacc ggcggggcat cgaatccctt cagcgcgttc
     6421 ttcgtggtgt acgtcgcgtt ggcggcgctg gtgctggggc gcgactgggc gatgaccctg
     6481 gtcgcgttca cgagcctgtg ctttggcgcg ctgttctggg ttcccgccgc gtggcttggt
     6541 ccgcacgcgc accacgtggc gacgccgcag cacctgcagg gcatgtggac ggcctacgcg
     6601 ctgtcggcct gcttcgtcgc gtacttcgtg tccaaaattg ccaacgcact gcgcgaacgc
     6661 gaccgacgca tcttcgattt gaagcgctac gcggctgcca ccgaaaggct cgccagcctc
     6721 tcgaccatgg ccgcgggcgc cgcccacgag cttggaacgc ccctcgccac aatcgcggtc
     6781 gcctcgtccg agcttgcaga cggactgcgt gcgaacgccg accccgcgtc gttgctgtcc
     6841 gaagcccact cgattcgcaa agaggtcaat cgctgccgcg agctgctcga tcgcttggcc
     6901 gcgggcgcgg gcgtcggggc tggcgaaagg ctcgagtttc acgcgctcag cgaagtgctg
     6961 gagaacttgc acaaggagct cgggaccgag cgcgccaagc ggctggtcct cacctgccga
     7021 gcgcccgccc aaacccggct gcgcgtgccg ctgcgcgcgt tgactcaagt tctcgtgaat
     7081 ctcgtcgaca atgggttgta cgcgcaacga atcggtggaa acgacgcggc gatcgagctg
     7141 gagctcgatc tacgcacagg ccaatcgtct gcttcgcagc tcggttttcg agtcctggac
     7201 cgcggcgtcg gcttatcgag cagcgagctg ggccggatcg gtgagccgtt cttcacgacc
     7261 aagccgcaag gtgaaggcat ggggctcggc ttgtacttgg tgcgcaggct cgcgcaggat
     7321 ctagaaggcg atttcagcat cggagcgcgc gccggggggg gcacggaagc tttactcgcg
     7381 ttgcctctgg atccggtgaa tgcccggcgg gaaacagagc agatggcggg gaccagcacc
     7441 ctcaatcctt ccgtcttgga gtcaagtatg gctcgacgcg agtgcggggc acgcgctgca
     7501 cctccgcgcg tcagtgtgcc ggagtcgaga cgggccgcgc gcgatgtctg agctcaattc
     7561 atccgagaat agcgttctcc tggtcgatga cgatctcgag ttccgctcca ccttggcgcg
     7621 ggcgtttcga aggcgcggct gggaggtctt cgaagccgat tcgccggcgg gcgcactcga
     7681 gctcgcccga aatgagagcc cggaacatgc ggtcgtcgat ctgcgcatgc cagagggaga
     7741 cggcatcgcg ttggtgcgtg cgctacgtgc cttggatccc gcgctgagga tcgtgctcct
     7801 cacgggtttt ggtagcatcg ccacggcggt cgagtgcatg cgcgcaggcg ccatcaatta
     7861 tctcaccaaa cccgcggacg tcgccgacat cctgctggcc ttcgaggggc agcagcagaa
     7921 tcccgagatc gtcgtacctt cgctggaccg cgtggagtgg gagcacatcc agcgcgtcct
     7981 gcactcgtgc ggcggcaacg tcacgcaagc ggcacagcta ctgggcctgc atcgccgatc
     8041 attgcagcgc aaactgcggc gcaatccgcg cgcgcgctga agcgcacccg gactcgattc
     8101 gtagctgtca gcggccgggc ttcttcaaca gcccgatgac caacgtcggg cgagcgccgt
     8161 cgatttctac gcgcctcgta acgtaccccc gccgtacgac ctcgaccgcg acgtgctttc
     8221 cgttgggcac atcgaagaag tagggcggcc ccttctgagt caaaccgtga agcgagacct
     8281 tggcgtcgaa cgggaccacg tccaaacgca cgcggcgctt cgcagcgcca gcctctttga
     8341 gggtcgcggc agacgttgcc tccggagctc cggggttcga ctctgtattc gggttcgcat
     8401 ctgtattcgg gttcgcagtg tctccgttcg actcggcgct ggcactcgca gccgctgggg
     8461 aatcagttgc gtctgcactc gagtcactgc cggaagcggt cgtctcggta ctggtggcga
     8521 ttgcactatc cgcagcagtg ggtggaagcg cggctgtggc ggcctcgacc acagcggacc
     8581 ccaccaagct ggcccggtcc atccccagcg ctaggccctg cgcgtccgaa ttgccacgcg
     8641 agcgagcgtc agccgccggg gtagcggctt gcgtctttgg atgtcggggc aactcacggg
     8701 gcggccaggc caaccgaacc agggtgaaag cgatcacgct cgaggccagc gcaaggcccg
     8761 cagccaacac aatctgccgg ccgcgtcgcg gcttgctctc gatagacgcg ctattcgcag
     8821 ctggcagaac ccgttcacga ggaccgctct cggttaccgc gccatcaccg tcagtgctcg
     8881 cgaggtgaac accttctgcg gggttgatcg ccgcgcgatc cggcggccca gcttgcgtcc
     8941 gttcggcggc tcgcgcagcc atgcgaccac ttgattggct ggctgcatcc cggaaggccg
     9001 cgcgcgcgcg ctcactggcc tgctctgagt caccccgctt gcccaggcga ccagaagatg
     9061 ccacggctga gtcgggctgt gctcgatcat cccaatgctg cgccgtatga tcgccgtgcc
     9121 gcgatgccat ctcctctcgc cggagcgccg ggcttgcttg cggctgtgcc gcatgggcac
     9181 cacgaacctg gaggtcccgc gccagcggga acggttcgcg aaacccagcg gctcgccagt
     9241 cgagcggatc ggccgcggag gaatcggtgg ccgcccccgg gctgaggggc tgagcgtccc
     9301 cggaacgcac cacgaccatc ggggccgcgg gtcgacgtga gctctgtggc cgccagtccg
     9361 ccggcgcggg cttgatgctc gaacgacgat gcgaaatcga ggtcttctca tgcatcagct
     9421 cggcaccaca ctccatgagg cccggctcga acggttggag cgggctgaac ggcgacgcgg
     9481 aactccgctc ttcgcgcgat cgtctcgctc ctgagcgggc tccaggctcg gaaacaggcc
     9541 cggagactgg gctctgcagc tcagcccgac tgatcgggtt caattggctc gcgatgactg
     9601 gctcagggtc gctgatcgca gctccgtccc gctccacacg ctctgccgca tccacatcgc
     9661 tggaaggcgt gttctgagtc cgctctgggt ccgacattac cctcacgcat agagtgcgcg
     9721 ctggcggcgc cagcttcaag cgcatttacg cgcacaatgc cagatccgag caataccccc
     9781 aagggtccac ctcccggggg tcccgagtgg ttggcaagag cgtgtcccgg attcatggca
     9841 gccggcgtcc gtttcgagtc gggccgcgtt caatccggac ggcgtaccgg gccacgttca
     9901 ataagaaccg aagcctacgc cgtttcatgt actcggtgat tttgtgtcgc tcaggcgatt
     9961 tgactacttc cttcggagca gtttcgacag gagacccttt ctgtcgcaga gctcctgctt
    10021 cgtctcgaga gccgtgggga cgactgcgac atggccgtcc ccaacgaatg cgcgcgagag
    10081 gggtggtggc tcctgccgga ggcgaccggc ggtgaaacgc cttggcgtta cccgccctga
    10141 cggttggccc ggcgctggcg gttagggcgc atagccacga acatgcgggt tcaaggagga
    10201 cgaacttgcc cgcatgatca acgcgagttc agcgaggcac gtcacgtccc gcggcgtggg
    10261 tcagccgcag atctgcgatt cgcaaatcga ttcccgcgtt gagctcgctg atgcgcgccg
    10321 agagcagctc ggtctcggcg tcgatgactt ccgtcgtcgt ggccttgcct acacggtaca
    10381 ggtccgaagc tacacgatac gcttcctgtg cggcagcgag gccccgacgc gctgactcca
    10441 aagcgcctcg cgcccgggtg agcgccagat actgatttgc gacttcctgt cgaattgcgt
    10501 cgcgcagctg cgcacgctgc gcttcgagcg aacgggacgt cgcggccaag tcccttgccg
    10561 aggcgttgtt gacgaacacg tctccaatcg tccagctcgc ggcgatcccc accgaccaag
    10621 tcgatttcca ctcggcccgc tgcgggaagt agcgctggtt ggggttcgcg taggtgacgt
    10681 cacccgtcgc gtccacccgc ggataccccg cacgttgaac ggcctttgac gccaggcgcg
    10741 ctgcctcgga agcttcaccc aatgcttgga gctcgatccg gttggagaag gcctcgcgcg
    10801 tgagagccgc gagagtgtct cggcgctctg cgggcaaaga ggaaatgtct tcgccgtttt
    10861 cgtaagtacg tggctcggcg tcgcccaaga acaccgcgag ctgcacttcg tccatgtctt
    10921 ggtaagcgcg ggattcgagc accacctgct cggtggcggc ggcttgcgct tcgagacgca
    10981 tgacgtccgc gcgcgtgatg gtgccgagtt cgaacgcgac ccgcgcatct tttagacgag
    11041 cccgcactcg gtccagcgac ttttccgaga tacgaacgcg cgccttggcg cgcaaccaat
    11101 cgtagaacac cgctctcgcg tccgtctgga ccttgagccg ctcggctttc gcgttcagct
    11161 gcgaagcgcg ctcggtggcg gcggcccctg aggtcgcggt cgagagacgt aatacgtaat
    11221 cggagattgg taccgagagc gacgccgtaa gcgcgtagtt gttttgcaga gcaggaaatg
    11281 ccagcgattg ggcgccaatg ggagttcctt ccgagtccac cacggggcac cgttcgagct
    11341 gacaattcgg atcgacgaag acaccaccgt tggcttgcgc cccgacgacc cctcccggcc
    11401 cgatttggga cttcactttc gagagccgcg tgtacgacgc ccgcagcgtg agccgcggga
    11461 agaactgggc tgtcgtttgg tcgagcttcg cgcgagcgga gtcgagctcc gcgaggcgca
    11521 cgcgaacgct ttgactcgtc tctgcgctgc gctcggcgac gtgctgcgca gttaaaccac
    11581 cgggttgagc gacgagcacc gccgcggctt catcgttggg ttcagttgcg ggcgcaggag
    11641 cggctgcgcc cgtagccggc gccgccgctc cgggcgtagc tccgatcgga gcccgcgcac
    11701 ccggagccgt caacgttgcg ggaggcggag tcgcggcggg aggagttcca gcaggctgtg
    11761 ttccagccgg cggcgttgcc gcgggcgggg tcgcacccgg cgcgcgtgca cctggcgcgg
    11821 gccgagcggg agcgccttgc gcttgggcct cggaagagaa cagctgcgcg ctcacgaatg
    11881 cgaggacgag ggcgctcaac gcaggcttca cgccgcgtcg aaccgcgtat ggtggaagct
    11941 cacggaccag gtgtttgcgg ttcgtcatgg cgcgtgcact atcgctttcg tttgggtgtt
    12001 cgacaaggtt gttcgcgcgg gtatgtggtg ccgaaaggag cggaattcaa gccccttaca
    12061 gttgtccgta gcgatcgccg ccgatcgaag acccagtccg atctcacgga caactgtccg
    12121 cactgaagtg gcatgtggcg agctctggac ctgcgcctct cgcttcctac acccgctgac
    12181 cgtcccgtcg gccgcgcaca ttcagagctg atctgctcga ctcacgctga gcgtctgccg
    12241 cgagaggctc ggcaggcgca acaagaactc ttcatgaatc gagagccgtt ctaagctcga
    12301 gcatgccgcg agatccccga gtagagattc aatattgccc gcgctgccgt tggctcttgc
    12361 gtgcgggttg gctggcgcaa gagctgctca cgaccttcga gggccaactc ggcgaggtgg
    12421 ccctcgtgcc tggggcgagc ggtgaattcg atatctactt ggggcacgaa ctcctgttct
    12481 cgaggcgtgc tcgaggccgc tttccggaag ccaaggagct gaagcagctg attcgggatc
    12541 gcattgcgcc ggaccgctcg ctcggccaca gcgatcgcaa gaccgagcct ctcgccgagc
    12601 agtcccccga gtcgaagatg tccgaccgcg ctcccgctgc tgaacccgct gcccacgaag
    12661 ccggcgagcc cggaaacgaa gctggagact actgcccgac cgattgatta gtgcgttccg
    12721 gttctcggac gcagtcgtct cgcgccgtcg agaccacgcg cgggcacccg agggggctag
    12781 gcgccgccat aatttcgcca caattcgtcc atacgaactc cgtagcaact ccgcacttcg
    12841 gtgcgttgga acagcctaga ttcccagacg tggccgcccc gcgcatgcgc gggctcgatc
    12901 gcggaccaca ggagaatctg catgaattcc gcgtttccca gcagaggcac ccccggcgag
    12961 gcgcatgctt ccgccaccgc cgcgccgtca ttaccgcacg ttccgagtta ctcgcccact
    13021 cggcttcccg acggcgcttc gccctccccc gctccatctt cctcggccct gtcttcatca
    13081 gcgccgtccc catacacctc ttcgccgaac gacgaaggca cgattcgcga tcacagcgag
    13141 gcgacggctg ccccgatttg gcagcggagt gagcgagacc aagctgaaac attggcgcga
    13201 gagtctgaat tcgagtggcg gccctggctc acgcgggttg cgagcggctt cggtctcgca
    13261 gctatctatg gagcggcgct gggcagccgc gcgggcgggt tctcgttgct gcgacacgcg
    13321 gtcgcggtgc cgctcggctt gctcggggtg agcgcggtag ggctgttcgc gttgttggtt
    13381 tccttgtcac tgctcgatgc tcaatcctct ccgggacaaa tcctgtccgc cgcggcccgc
    13441 agcatctcga gcacggggct gattctggcc ggcatcgcac ctgcaacggc gttgctcgtg
    13501 gtcaccattc gagaggcagg cgcggcgcaa gccgttgccg ccggcggctt actcttggcc
    13561 gccgcggtcg gaattctgcc agccctgaag gtaacctatc gagccttgag ccccgaggct
    13621 tggccattca cgctgcgcgc gccgctgttc gtcactggct ttgcggtgtt cagcttcgcc
    13681 ctcggcgcac gcctgttctt ctcccttttg cccgtgctcg cgggaggtgc ctcgtgagtt
    13741 ctacgctcgc tacactcgac caattgctgc gctctccaaa ccgcgccgcg gagcaaagcc
    13801 gcggcggcgc tcagctgcag aatctagcgc tcgcgtcgct cggagcttta ctgttcggcg
    13861 ccggtctatt cggagccgtg ctcgccagtt cgcgtggcgg attgcagctg ctgtattccg
    13921 ggctcaaatt gccactagcg atcctcggca cactgatcgt gagcgtgccc gcgctgtacg
    13981 cgctggcgcg cagcctcgct cgcccgctgc cattggcggg cgcggtcagt ctggcgctct
    14041 cggcggccgc tcgtggcgcg ttggtgctct cggcgctcgc tccggtggca tggctcgcga
    14101 tcgaccgagg gctgggctac cacggctgcg tcctgctcgc ggccatttgc tacgcaattg
    14161 caggtgtttc ggcgctgagg gtactgttca gcggcgtggg aggcggccta cgtggcgccg
    14221 tgctgcttgc gctctgctca atcgtggtgc taccggccgg cggccaaacc gcgtggatgt
    14281 tgaggccgtt cgtggggcgt cccgcacaag ctcatgtccc atttctgcgc cacaaggagg
    14341 gcagcttcgc ggattccttg ctgcaaagcg ggcgatccag cgtgggcctc taccgaacga
    14401 tgtcttcgga agaggcggag gagttcgagc ggtgaccctc acggatctcg gaattctata
    14461 cggcctctcc ggcgtgatga gcgcgatctg gctgcgccga cgcccggtgg ttggcgtgag
    14521 gccgagcctg ggctccacgc tgctcgtcgt gctcgtctgg ccactctggg tccccgtcgt
    14581 gctcagcgcg gagcgcccga acccggtggt cacccagcga acctcgctcg agatggaatc
    14641 ggcactggtc gaagggcatc aagccataca aggcacgcca ctcgaaccgc tgctgccccg
    14701 cgccgcgctc gagagaatgc agacggaggt gcgtcgagct gcagagcgcc tggccgagct
    14761 cgacactctg ctctcgagcc cggccttcga tcgtacgcaa gcgctcgagc acctcgagcg
    14821 tttggaaagg cgcggagcat cgggtcgcac tctcgcatcg gcccgttcgc attgtgagag
    14881 catcgagcgc ttggcggcgc tccgcgaccg agaccgcgcc atcctggagg aagtttctga
    14941 gctttcgaga gcgctccgaa cccagctggt gctgtcccgt taccgaggct cgtcgcccag
    15001 cgacgccggt gatatcgtct ccgagttatg ggcaaggctc gaggtgttgg atatcgacag
    15061 cgctccgctg gcacggccgc ctgacaatga cgcgcgagaa gctaatcctg gtcgtggatg
    15121 acgagaaacg cattcgagac gtcgtcgaat atgcgctcgg caaagatggc ttccgagtcg
    15181 tgtgcgcggc tcacggaacg gacgcgctaa ccgcggtcga gcgcgaggcc ccggatctga
    15241 tcgtgctcga cgtcatgttg ccggggctgg acggcatcga actgtgccgc cgtctgcgta
    15301 cgcgcagcaa cgtcccgatc ctgttcttgt ctgctaggag tgacgaggtg gaccgcgtgg
    15361 tgggcctcga ggtgggcggc gacgactacc tcacgaagcc gttttcaccg cgagagctag
    15421 tggcgcgcgt gcgggccgta ttgcgccgat tcagcaccgg gcaaagcccg aacgaaatag
    15481 cgagctccgc agcggatccc ggagctcgct ccacgcgaat atcgcatgga tcaatcgatc
    15541 tcgatccttc gggttggacg gtgcgctgcg gcagccagag catcgcgctc accgcgacgg
    15601 agttcatggt gttggcggcc ttgctcgagc gccccggagt cgtgctcact cggcaacaac
    15661 tgatgcagag aacccacgtg agcggcgtga acgtgacgga gcgcacgctg gacacccatg
    15721 tgcgccgcat tcgcgcgaaa tttcggccat tcggcgtgga tcccatcacc accgtacacg
    15781 gcgtgggcta caaggccacg ccggcttgaa tcatggtgtg gctcaccacc aagtttcgtg
    15841 ctcggctggc acgcgtgctg cgcgagttcg ggcgaattcg ttaccgattg ctggccgtca
    15901 acttgttcat cgtactcgtg cccgcggccg ggctcgagtt tgcacgcgtg tacgaacgtc
    15961 agttgctcga aggtctcgag cgcgacatgg agaatcaagc cagcttggtg cgcgtggcgc
    16021 tggagctccg ggggtcgctc gacctggaaa acaaggtgga actgaaggag ctcgagcaat
    16081 ggctcgaacg cgcggcgcgc ggaacgcgca cacgtgtgcg ggtcgttcac gcgagtctgg
    16141 gcgtagtgct cgactcgcac agccacggcc cgcccgaggg gccgcgcttc gatcccccac
    16201 gcctgaccgc gttcgcgcac agccgtctcg ccagtgaggt cgctcgcgcc tccagctttc
    16261 cgacatccat ccaagagcgc tccgagcttc gcgcggcgtt ctcgggcaaa cgcgccacgg
    16321 cgacgcgagt cgcgcgcaaa ccaccggcgg tctatttgtt cttggccgat cccattttgc
    16381 gggagggcaa agtctgggcc gcggtgtatg tgacgcgctc gaccgaacca gtgctggtcg
    16441 aactacatcg cattcgtcgc ggcttattga ttgtcctggc cgtagcgctt gccatcggtg
    16501 cgggcaccac gctgttgctc gcctggacca tctcacggcc gctcgagcgc ctggcgtctg
    16561 ccgcccgtcg tatcgcgagc ggcgccgtcg aggtggatct gccctccagc ggtggcggcg
    16621 aaatcagcgc gctatcgcgg gcgttcggcg agatgacgcg caagttgcac gagcgccacc
    16681 gctatatctc ggagtttgcc gcggatgtgg cgcacggctt caagtcgccg ctgacttcca
    16741 ttcgtggcgc ggcggagctg ttgaacgaag gggccgcgca agagccggaa gcgcgcgacc
    16801 gtttcctagg caatatcgtg caggacgcag aacgactcga ccgcttggtc tcgaggctgc
    16861 tgcaattgag ccgcatcgat tctgccgaca cactgccggc gtggatcgag ctcgatgcgc
    16921 tcgtcaaacg agtactgcgc cgcttcgatc gagccgatgc ccctgtgcac ttgctgcaga
    16981 actcgagcat cccggtgctg tacgcacgcg aaggggactt ggaaatcgcg ctcggcaacc
    17041 tgttggacaa cgcgcaacgg catgcgccgc ccggtagccc tatcgagttg cgggtcagcg
    17101 gaagaccaag tgatgggaag gtgaccttct cggtgcgcga caccggcgcg ggtattgcgc
    17161 ccgaacacct tccgcacatc ttcgatcggt tcttcaccac cgactccgag ggccagggaa
    17221 ccggccttgg gctttcaatc gtgaagagtg ttgcgctcgc acacggcggg cagatcagcg
    17281 tgttctcggt accgggccag ggggcgcgct tcgacctctg tatcccggtt gcccccagca
    17341 acttggttgg gctccgctcc tgagttgagc ttagccctcg ctcatacgtc ggcgcgtcag
    17401 ccaatccgag tcgacttcga tgcgcccgat gggctcgacc ggtccctcga gcaacaagcc
    17461 tcgctcactc gaggtcacga acagctcgcc ccccggcatg ctcacgcgaa cacgcccggc
    17521 tctgagccgt cccgtccgca cgcccgcggc gaccacaccg caagaggaag agccagaggc
    17581 ttgggtctcc cctgcccctc gctcgtgaat tcgaatctcc acttcgtgtt cggagatgac
    17641 ccgtgcaatc tgcacgttgg tgcgattggg aaacaactcg tggcgctcca gctcggcccc
    17701 ccaacgacgc cacggcaaac cgtccagcgc ttcctcgcgg aacaccacgc aatgcgggtt
    17761 acccatgccc accgcggtcg caacgacact ctcaccctgc agcgtgatgg cgcgctcgat
    17821 ccactcgttt tctgagcgga tcgggacatc cgatggcgca aagctcggta gccccatttc
    17881 gacgctgatc tgccctcggt ccgcgcggca accgacttta cacgtgcccg tccagagcga
    17941 gaacacggat ggtgccccgc gctctttcaa caaccaacgc gcgaagatcc gcagcccatt
    18001 gcccgatttc tcggcaatag aaccgtcggg gttccagatc ctcacgccga aatcggcgtg
    18061 agccccggcg tcgaagggct ccaggatgcc gtcgccacct actccactgt ggcgatcgca
    18121 aacgtcacgg acacgctcgg gagatagagc gtccgccgtc tcgagtacca ggtagtcgtt
    18181 gcccagtccg tgggctttcc agagtttcac ggcgccaggt tttacatcaa gtggttcacg
    18241 atgccacgcg ctcttctcgc tcgaagaatg cgcccggatg agtcaaagtc cgacgcagtg
    18301 tctgtgtccg agctgccctt cgcgtacgcc agccctcgcg ccccccagag catgggggtg
    18361 tacgtacact tcccttattg cctgcagaag tgtccctact gcgatttctt gagcgtgccc
    18421 gtcgaacgcc cgcacatccc acagcagcgt tacacggacg cggtgctcgc ggagctggaa
    18481 gcccgtcgcg cagagttgcc gccgctacct atccacagca tttttttcgg cggcggtac
//
LOCUS       DASTKU010000883         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_841574, whole genome shotgun sequence.
ACCESSION   DASTKU010000883 DASTKU010000000
VERSION     DASTKU010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841574"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1226)
                     /locus_tag="VFQ61_31105"
     CDS             complement(123..1226)
                     /locus_tag="VFQ61_31105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9958995.1"
                     /translation="MDQHKPSTAQQVASRTLNVIGASLEQELEVRTRPGKLWSSADGE
                     AVRCYACAHRCFIQNGSRGACGVRFNRDGVLQVPFGYVARRYVRPVETNTILHVLPGA
                     RALTFGMFGCDLRCPYCHNHRLSQALRDGPSAERPVDMSPDALVEEALRAGCSVICGA
                     YNEPMITAEWGHAVFEVAKRHGLKTALISDGNSTREALEFMRSVTDVFRVDLKAADDA
                     GYHRLGGRISPVLESIRAARELGYWVEVVTLVVPGLNQDLGSIRQIATTLAAIDPEMP
                     WHLNGFVPRYKLQQMPAADPFFLSSAAGSGYAAGMRYVYVGNLPGRDELCHTRCPSCH
                     QVLIRRQDYATLENELVQGRCRQCGTNIPGLFS"
     gene            complement(1610..2629)
                     /locus_tag="VFQ61_31110"
     CDS             complement(1610..2629)
                     /locus_tag="VFQ61_31110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9958996.1"
                     /translation="MTSRRQFVVGITSAAAAVACRSKQQRDNPAMATDTRPIPRRPLG
                     KTGVNVSILGLGGFHIGKSKDEKESIALVRRAIDSGITFLDNCWDYNDGESELRYGKA
                     LRDGYRQRAFLMTKLDGRTQAAATAQLEQSLQRLQTDVIDLVQVHEVIRDTDPGRVFE
                     KGGAIYALIEAKKAGKLRFIGFTGHKDPKIHLAMLRAADEHSFKFDTVQMPLNVFDAH
                     FRSFEKEVLPVLVEQGIGVLGMKSMADGRILKAGVVSPEECLRYALSLPTSVVISGMD
                     KPEILEQNLRVAREFKPLTDDERKSLLARTAKPASDGAYETFKTSQDHDGTVEHPHWL
                     EEARI"
     gene            complement(2648..>3409)
                     /locus_tag="VFQ61_31115"
     CDS             complement(2648..>3409)
                     /locus_tag="VFQ61_31115"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9958997.1"
                     /translation="ARPREGYGRLLSYLEREIVEAGGELELLVKVEGVECSGRVQAVS
                     RAGVWGAAAAVVALPLSILKAKSELGGISFEPRPRGLRDVLSRFEMGHALRVVFRLRG
                     PITNAHERSGSAFLHAPQQAFPTWWTSRRVGCWQLTGWCGADKAARWDGEAPEKIVNA
                     AQDSLRAVLGCSESQLKSAIISAQLYDFGADPNLRGAYPYARPGADAEAILPPDLAGQ
                     VPLWFAGDYMVPAYLGTVGAAVQSGMAAARALLRR"
BASE COUNT          670 a         1169 c          935 g          635 t
ORIGIN      
        1 tcctgcgagg tgccgaaccg cagctggatc cggtaccgct cgcagacatc gcggaggagc
       61 cgcgtacgaa ttggccggcg tggcgtccaa cctcagaacg agcgcgcgtt ccacgttagc
      121 ttctaggaaa agaggccagg gatgttcgtc ccacattgac gacaacgccc ttgcaccaac
      181 tcgttctcca gagtcgcgta gtcctgccgc cgaatcaaca cctggtgaca gctcggacag
      241 cgcgtgtggc atagctcgtc tctccccggc aagttgccca cgtacacata gcgcataccg
      301 gcggcgtacc cggagcccgc cgcggacgag aggaagaacg gatctgcggc tggcatctgc
      361 tgaagtttgt agcgcggcac gaagccgttc aaatgccagg gcatttccgg atcgatcgcc
      421 gccaacgtcg tggcaatctg ccggatgctc cccaagtctt gattcaagcc gggcaccacc
      481 aacgtcacta cctccaccca atagccaagc tcgcgcgccg cgcgaatgct ctcgagcacc
      541 ggggaaattc gaccgccgag tcgatggtac ccggcgtcat cggcagcctt gagatccacc
      601 cggaacacgt cggtgacgct ccgcatgaac tcgagagctt cccgagtgga gttgccgtcg
      661 ctaatcaggg ccgtcttgag cccgtggcgc ttggcaacct cgaacacggc gtgcccccac
      721 tccgcggtaa tcatgggctc gttgtaggcg ccacaaatga ccgaacaccc cgcgcgcagc
      781 gcctcttcga ccaatgcatc cggcgacatg tccacggggc gctccgcgct cggaccatcg
      841 cgtagtgcct gcgacaaccg gtgattgtgg caatacgggc aacgcaggtc gcaaccgaac
      901 ataccgaagg tcaacgcgcg ggcccctgga agaacatgca gaatcgtgtt ggtctcgacg
      961 ggacgcacgt agcgccgtgc gacatacccg aacggcactt gcaacacgcc gtctcgattg
     1021 aaccgcactc cgcacgcccc gcgactgcca ttctggatga aacaacgatg ggcgcaggcg
     1081 taacaacgta cggcctcgcc gtcggccgaa gaccatagtt tgccgggccg ggtgcgaacc
     1141 tcgagctcct gctcgagtga cgccccaatc acgttcaacg ttcggctagc aacctgctgc
     1201 gccgttgacg gcttgtgttg gtccacggtt tctccgacgc cttccaccgc tttacggtct
     1261 atctaccgtt cgcctgccgc ggctcccgaa ctgcccatct ggaactgccc atctggaact
     1321 gcctatcccg aactgcttac aaaaagcgga ccgccctcgc gagtccttag cctctcatgt
     1381 ccaacacctt cgacgtccgc gtcaacacga aagcacctcc gatcggctcg aatgcttcgc
     1441 tttgcaaaac aaccgcaggc gtgtccgcga taaacgcctc tcctccaggt gagtgaaagc
     1501 acccctgaat cgatgcgctt cgagcgcaag cggccgcgcc gtgagtgcgc tcgaagtagc
     1561 gatcgaccgc ggcacgccgg gccggactct gcctgcaaga aatctaggtt tagattcggg
     1621 cttcttccag ccagtgcgga tgttccacgg tcccgtcgtg atcttggctc gtcttgaacg
     1681 tctcgtaggc cccatcactc gcgggctttg cggttcgagc gagcaagctt ttgcgctcgt
     1741 catcggtcaa aggcttgaat tctcgagcaa cgcgcaggtt ctgctccaaa atctccggct
     1801 tatccatccc ggagatcacg accgaagtgg gcagactcag ggcataacgc aggcattctt
     1861 ccggactgac cacacccgct ttcaagatgc ggccatccgc catcgacttc atgccgagca
     1921 cgccgatgcc ctgctcgacg agcaccggta atacctcctt ctcgaagctc cggaagtgag
     1981 catcgaatac gttgagcggc atttgaaccg tatcgaattt gaaggaatgc tcatccgcgg
     2041 cgcgcagcat ggccaaatgg atctttggat ccttgtgccc cgtaaagccg atgaagcgca
     2101 actttccagc cttcttggcc tctatcagcg cgtaaatggc gccgcccttc tcgaacacac
     2161 gaccaggatc ggtgtcccga atcacctcgt gcacctgcac caggtcgatt acgtccgtct
     2221 gtaaacgctg taaggactgc tcgagttgcg ccgtagccgc ggcctgggtg cggccgtcca
     2281 gcttggtcat caagaatgcg cgttggcggt agccgtctcg cagagccttc ccatagcgca
     2341 attcgctctc accatcgttg tagtcccagc agttgtccag gaacgtgatg ccgctatcga
     2401 tggcccgcct caccagcgca atactctcct tctcatcctt cgacttaccg atatgaaaac
     2461 cgcctagccc cagaatcgaa acgttcactc cggtcttacc caaggggcgg cgcggtatgg
     2521 ggcgagtgtc cgtagccatg gcaggattgt ctcgttgttg cttgcttctg cacgcgactg
     2581 ccgccgcggc actcgttatc cccaccacga actgtcttct cgaagtcatc cgtcatctcc
     2641 tcgggcgtca tctccgcagc agggcgcgcg cggcggccat cccgctctgc accgcagccc
     2701 caaccgtgcc caggtacgcc gggaccatgt agtcccccgc aaaccagaga ggcacctgac
     2761 ccgctaagtc cggtggcaaa atcgcctccg cgtccgcacc gggtcgcgca tacggatagg
     2821 cgccgcgcag attgggatcc gcgccgaagt catagagttg ggccgagatg atcgcgcttt
     2881 ttagctgcga ttcgctacac ccaagcaccg ctcgcaagga atcttgcgcg gcgttcacga
     2941 tcttctcggg agcttcacca tcccaccgcg ccgccttgtc ggccccgcac caacccgtaa
     3001 gttgccagca gcctactcgg cggctcgtcc accaggtggg gaatgcctgc tgtggggcgt
     3061 gaaggaaagc agaacctgaa cgctcgtgcg cgttcgtgat cggcccccgc aatcgaaata
     3121 ccacgcgcaa ggcatgaccc atttcgaagc gggacagcac atctcgcaag ccacgggggc
     3181 gtggctcgaa actgatacct ccaagctcac ttttcgcctt gaggatggac aagggaagcg
     3241 ccaccaccgc cgcggctgcg ccccacaccc ccgcacgcga aacggcctgc actcggccgc
     3301 tacactccac cccttccacc ttcaccagca gctcgagctc tcccccggcc tcgacgatct
     3361 ctcgctccaa gtagctcaag aggcgcccat acccctcgcg tgggcgggc
//
LOCUS       DASTKU010000884         5583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_394582, whole genome shotgun sequence.
ACCESSION   DASTKU010000884 DASTKU010000000
VERSION     DASTKU010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394582"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="VFQ61_31120"
     CDS             <1..1432
                     /locus_tag="VFQ61_31120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958998.1"
                     /translation="RIYPQTALCASGAAPCTTCHTTPPALNAYGLQVERVLSPDAPRP
                     LSDELFLSGLAAALRAAAPLDADGDGASNVAEFQAGTLHVDDTSQPSRMIGCDSEQRA
                     RASAQPWNTCAYDPAYALRRIGLDFCGHSPTRGELEAVRASNDWQMLLEQKLASCLDS
                     AFWQGKNGALWNLANAKILPESSLKSGDGAADITLADYEDDYNLFVYINTDDRDVREL
                     LTAKYHAIRQPDGTLVRVDRTPVEDILARESDDNVDVAQLVPQDRRAGMLTTRWFLAK
                     NTMFTPIPRTTAAQAYRAYLGYDIAKLEGLRPVAGEPVDYDRKGVAAEQCAVCHSTLD
                     PLSYSFSRYDGIGGGDEEEISDPDMAINADGTINGTFGNYASERLERFVRQEGPQIVD
                     TPEAGLLLGQPVANLVEWAEVAANSEPFARKVVLDYWRLLVGEDPQPGDEAEFARLVQ
                     RLSSEHEYRVERMLRDLIATEAYGAP"
     gene            1422..2078
                     /locus_tag="VFQ61_31125"
     CDS             1422..2078
                     /locus_tag="VFQ61_31125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9958999.1"
                     /translation="MHPERIPGILIVTFTFLAVASACTSESKNGVPRERVPTAPAEPV
                     AASHQARLKYKSGARYANDLAAALDLERNAICRELGRYDCVDEVHRIVLGGVEPYTLG
                     VREPLPSIAVTAPIAQDRVALAACTERIERDLGALKPVFLTTLNVDAPTPAQLEATAK
                     RFYDRILLREATPYELSALVQFHSTVTDEPHESPATATRDWAILSCFMVATTLENVFY
                     "
     gene            2197..3714
                     /locus_tag="VFQ61_31130"
     CDS             2197..3714
                     /locus_tag="VFQ61_31130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959000.1"
                     /translation="MLGLGRHARAQDAEEEARARSADPRFLIVLSTTGGASMLDAMLA
                     VRESESANARTLNCFPDSTVRTVGPFRAVDIDLPNLGAIPMPVKGRQSAMVKAHQADM
                     MVATWQRTSVNHLIGQRRAVNGNSAWYGRTLQEAVALTYGARFALPNVHLSAGTGYND
                     RGSDSALPAYCYGETVADPRLWPLSLHGSHGILGAPSHYLINQARTLRNTRLDPQSRF
                     GRVFAESPRLKTWEHLRGSPQQALEAQDLITKLMISPESADFPLAAHGLDSSPDGALV
                     RSAFPEFERDPLEAQAALAFLLLKYRLTVTVTLGPSFSAVIHAEGAEAEDGMGAEVYD
                     SRMDDDPTNGGGLPEGSIANPPLSFDFSHSEHRSVQALMWSRVLGVADRLIRLLKSEE
                     WSNGQSLWDRTMIYVANDFGRSKQRPSGAEQFGTGHDLNDGALIISPLANGSRLLGGI
                     DADTGNTYGFDPATGEPDRGRQMTEAEIYAGTAQALGVSLDGVSLPDMRAMRRRA"
     gene            complement(4069..>5583)
                     /locus_tag="VFQ61_31135"
     CDS             complement(4069..>5583)
                     /locus_tag="VFQ61_31135"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9959001.1"
                     /translation="DEIVLAGGMYELTERFGISVAGTREAPIVIRATEGEKPNLHRQG
                     ADQNTIDVDDATHVVFRGLEISGGSHGLRLVKASFVTIEDCEIHDTADVALSANSGGS
                     YEGLRILRNHIHHTDGTGEGMYLGCNQDVCRMFDSLIEGNYVHHTNAPTVVQGDGIEI
                     KEGSYNNIVRDNVIHDTNYPCILSYSTVGNGAPNVIERNVLWNCGDHAIQSAADAVIR
                     NNIILGSGANGIAMQQHQSGAPSNLVVVHNTVLHATNDAISARGITGSVVIANNALYA
                     QSGRAISVGGELGQLTLAGNYGVGAFDGASGEFDGSGDLARDFVAASFSGALPNDVFP
                     APGSGLIGAGSLAHLVMDDFNGTQREGVADVGAYAFDPSGNPGWPLGAEPKGERPVAG
                     AGGSAGAVAGAGGGPSSGGTRASGGGGGTRASGGGGGTRASGGGGEPGGRGSVGGPMP
                     SGDEGGCACRVEGGGRAASVSWLAGLMLVGLTVERRRRSSRAPRFGARAAMRQY"
BASE COUNT         1016 a         1854 c         1723 g          990 t
ORIGIN      
        1 ccgtatctat ccgcagacag cgctgtgcgc gtcgggcgcg gcgccgtgta cgacgtgtca
       61 cacgacgccc ccggctctga atgcctacgg tctccaggta gagagagtgc tatcgccgga
      121 cgcgccgcgc ccgttgagtg atgagctgtt tctaagtggc ctcgccgctg cactgcgcgc
      181 tgccgcgccc ctcgacgccg acggtgacgg cgcgagcaac gtggcggagt tccaggccgg
      241 cacactgcat gtcgacgaca ccagccagcc cagccgtatg atcggttgcg attcggagca
      301 gcgcgcgcgt gcttcggcgc aaccctggaa tacttgcgcc tatgaccctg cctacgcgtt
      361 gagacggata gggttggatt tctgtggcca ttcacccacg cggggcgagc tcgaggcagt
      421 tcgagctagc aacgactggc agatgctgct cgagcaaaag ctcgccagtt gcttggatag
      481 tgccttctgg cagggcaaga acggcgcgct ctggaatctc gccaatgcca agattctgcc
      541 cgaatcctcg ttgaagagcg gcgacggcgc agccgacatc accctcgccg attacgagga
      601 cgactacaat ctcttcgtat acatcaacac ggacgaccga gatgttcgcg agctcctgac
      661 ggccaaatac cacgccatcc gccaacccga cggtaccctc gtgcgcgtcg atcggacccc
      721 cgtcgaggac atcttggcgc gcgaatccga cgacaacgta gacgtcgcgc agttggttcc
      781 gcaggatcgg cgcgcgggaa tgctgacgac acgctggttc ttggcgaaga acaccatgtt
      841 cacgcccatc ccgcgcacga cggcggcgca ggcgtaccgc gcgtacctgg gctacgacat
      901 tgcgaagctg gagggcctgc ggcccgtcgc cggcgaaccc gtcgattacg accgcaaagg
      961 agtcgcggca gagcagtgtg ccgtctgcca cagcactctc gaccccctga gctactcgtt
     1021 cagccggtat gacgggattg gcggcggcga tgaagaggag atctcggatc cggacatggc
     1081 gatcaacgcg gacggcacga tcaatggcac gttcgggaat tacgcatccg agcgcctgga
     1141 gcgcttcgtg cgacaggagg ggccgcagat cgtcgacacg ccagaggccg gcctgctctt
     1201 agggcagccc gtcgcaaacc tggtggagtg ggccgaggtc gcggcaaata gcgagccctt
     1261 cgctcgcaaa gtggttctcg attattggcg gctgctagtc ggcgaagacc cacagcccgg
     1321 ggacgaagcc gagttcgcga ggctcgtgca gcgcttgtca tcggaacacg agtatcgagt
     1381 cgagcgcatg cttcgcgacc tcattgccac ggaggcatac ggtgcaccct gaacgaatcc
     1441 caggcatttt gatcgtcacc ttcacgttct tggcagtggc cagtgcctgc acgagcgagt
     1501 cgaaaaacgg agttccccgc gagcgggtcc cgacggctcc ggccgaaccg gtcgcggcct
     1561 cgcaccaagc acgcttgaag tacaagagcg gagctcgcta cgcgaatgat ctggccgcgg
     1621 cactcgacct ggagcgaaat gccatctgcc gcgagctggg acgctacgat tgcgtcgacg
     1681 aggtgcaccg catcgttctc ggaggtgtcg aaccctacac actcggcgtg cgggagccgc
     1741 tgccatcgat tgccgtgacg gcgcccatcg cgcaggaccg ggtcgccttg gccgcgtgca
     1801 ccgagcgcat cgagcgtgac ctcggagctt tgaagccggt gttcttgacg acgttaaacg
     1861 tcgacgcgcc gacaccagct cagctcgagg cgacggccaa gcgcttttac gaccgcatcc
     1921 tccttcgcga ggcgacaccc tacgagctca gcgcgctcgt gcaatttcat tcgacggtga
     1981 ccgatgaacc gcatgagagc cccgccacgg cgacccgtga ctgggcgatc ctttcctgct
     2041 tcatggtcgc gaccacgctc gagaacgtct tttactgagg agtccaccgt gagtaagcaa
     2101 accaagccac tagcgtcatt aggccgtcgg tcgtttcttg ggaggttggc gcagatagcc
     2161 gccgctggga catcgctcag cgtcggcggt ctcggagtgt taggcctggg gcgacacgct
     2221 cgagcgcagg acgcggagga ggaggctcgc gcgcgctccg cagacccgcg cttcctgatc
     2281 gtgctctcga ccacaggagg agcctcgatg ctcgacgcga tgctggccgt tcgagaatcg
     2341 gagagtgcca atgctagaac cctcaactgc tttccagatt ccacggttcg cacggtgggc
     2401 ccctttcgtg cggtggacat cgacctgccc aatctgggcg ctatcccgat gccggtgaag
     2461 gggcgccaaa gcgccatggt caaggcgcat caggccgaca tgatggtggc gacctggcaa
     2521 cggacgagcg tgaaccacct catcgggcag cgtcgcgcgg tgaacggcaa cagcgcgtgg
     2581 tacggccgaa cactgcaaga agccgtcgca ctcacctatg gtgcgaggtt tgccctgccc
     2641 aacgtgcatc tttcggcggg cacgggctac aacgatcgtg gctcggactc agcgctaccc
     2701 gcctactgct acggggagac ggtcgcggac ccaaggctct ggccgctatc ccttcacggg
     2761 agtcacggca tcctcggggc accgagccac tacttgatca accaggcacg caccctgcgc
     2821 aacacccgcc tcgaccccca gtcgcgcttt ggtcgtgtgt tcgccgaaag cccgcgcctc
     2881 aagacttggg agcatctccg cggcagccct caacaggcct tggaggcaca agacctcatc
     2941 accaaattga tgatctcccc ggagagcgcg gattttcctc tggccgctca cgggctcgac
     3001 agctcaccgg atggagcgct ggttcgctcc gcctttccgg agttcgagcg tgatccgctc
     3061 gaggcccagg cggcgttggc attcctcctc ttgaagtatc gtttgaccgt gacggtgacg
     3121 ctcggcccca gcttcagcgc cgtgatccac gccgaggggg ccgaagccga ggatgggatg
     3181 ggagcagagg tatacgactc gagaatggac gacgacccca cgaacggcgg cgggttgccc
     3241 gagggaagca tcgccaatcc ccccctgtca ttcgatttct ctcattcgga acaccgcagc
     3301 gtgcaagccc tgatgtggtc tcgcgtgctg ggggtcgcgg atcggctgat tcgcctgttg
     3361 aaaagcgaag agtggagtaa cggtcagagc ctctgggatc gcacgatgat ctacgtcgcg
     3421 aacgattttg gtcgttccaa gcagcgaccc tcgggtgccg agcagtttgg gaccgggcac
     3481 gatttgaacg acggcgccct gatcatttcg cctttggcca atggtagccg tctcctcggc
     3541 ggcatcgacg ccgacaccgg taacacttac gggttcgacc ccgcaacggg cgagcctgat
     3601 cgtggccgtc agatgaccga ggccgagatc tacgccggca ccgctcaggc cctcggtgtg
     3661 tctctggacg gcgtctcgct gccggacatg cgcgctatgc gtcggcgcgc ctgaggccaa
     3721 acccgcccag ggcctacgaa gcaaccaaag tatcgctgcg tcccggtcct catacccggt
     3781 gctgggtcgc gtacgtaagg gggatacgaa gggctggttt catctcgcta ctcgaccata
     3841 agccgttcaa agatacggtt gcggccgcgc ctagacctac atacgcactc gagacttagc
     3901 aacaggagct catatgagac cgcgcgcatt cggacagcca agatttcacc acgcttgggc
     3961 ctagcttcgc tcttctgtgg cgcactggcg ttccgctgca gcagaggcgc cgagccccgg
     4021 ggtgacgcgc gatagcccct agcgcaggta gcgcacattg tgaatagctc agtattgacg
     4081 catagccgct cgagcaccga agcgaggagc acgactggaa cgccgccgtc tttcaaccgt
     4141 gagaccgacg agcatcagcc ctgcaagcca agacacactt gccgcgcgcc ctccgccctc
     4201 gacacggcaa gcgcagcccc cttcatcccc cgatggcatc gggccgccca cgctgccgcg
     4261 acctcccggc tcgccaccgc caccgctcgc ccgcgtcccg ccaccgccac cgctcgcccg
     4321 cgtcccgcca ccaccaccgc tcgcccgcgt cccgccactg ctcggccctc cgccggcccc
     4381 cgctacagcg cccgccgaac ctccggctcc agccacaggc ctctcaccct ttggttcagc
     4441 tccgagaggc cagccgggat tcccggaggg gtcgaacgcg taggcgccca cgtcggccac
     4501 gccttcgcgc tgtgtgccgt tgaagtcgtc catgaccaag tgcgcgaggc tgccagcccc
     4561 gattagcccg gaacccggcg caggaaagac atcgttgggg agcgcgccac tgaagctcgc
     4621 ggccacgaag tcgcgcgcga ggtcgccgct gccatcgaac tcgccgcttg ccccgtcgaa
     4681 agcgcccacc ccgtagtttc cagccagcgt cagctggcca agctcaccgc caacgctgat
     4741 ggcgcgacca ctctgggcgt agagcgcgtt attggcaatc actacggagc cggtgatgcc
     4801 gcgcgcgctg atcgcgtcgt tcgtcgcatg tagcacggta ttgtggacca ccactaggtt
     4861 cgagggcgcg cctgactgat gctgctgcat ggcaatgccg ttggcgccgg accccagaat
     4921 gatgttgttg cgaattaccg cgtccgcggc ggactggatc gcgtgatcgc cgcagttcca
     4981 gagcacgttt cgttcgatga cgttgggcgc gccgttacct accgtgctgt agctcaggat
     5041 gcaggggtag ttggtgtcgt ggatcacgtt atcgcgaacg atgttgttgt agctaccttc
     5101 cttgatctcg atgccgtcac cctgcacgac ggtcggcgcg ttagtgtggt gcacatagtt
     5161 gccctcgatc aggctgtcga acatgcgaca gacatcctga ttgcatccga gatacatccc
     5221 ctcgcccgtg ccgtcggtgt ggtgaatgtg gttgcgcagg atgcgaagtc cttcgtaact
     5281 gccaccactg tttgcgctca gcgccacgtc agcggtgtca tggatctcgc agtcctcgat
     5341 ggtgacgaag ctggccttga ccaagcgaag gccgtgtgag ccacccgaaa tctccaagcc
     5401 gcgaaacacg acgtgcgtcg cgtcatccac gtcgatggtg ttctgatcag caccctggcg
     5461 gtgcaggttg ggcttctcgc cctccgtcgc gcgaatcacg atcggcgcct ctcgggtccc
     5521 cgctacgctg attccaaagc gctcggtcag ctcgtacatc ccgccggcta gcacgatctc
     5581 gtc
//
LOCUS       DASTKU010000885         5109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1290337, whole genome shotgun sequence.
ACCESSION   DASTKU010000885 DASTKU010000000
VERSION     DASTKU010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290337"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..519)
                     /locus_tag="VFQ61_31140"
     CDS             complement(64..519)
                     /locus_tag="VFQ61_31140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959002.1"
                     /translation="MTPKQNRKPGLAKTRTEPLVAERSISLIKSSDYRIRELFERADV
                     ISEGKTRLEAETAIYYGFSSILLKITSRGGLIPDVEAARVRALLARDPHARLRAVRIA
                     RVEAQVRAGSPIGRMKAELLVRLGEDGVLIDIDVEARVLEPRVRRRSRG"
     gene            653..1024
                     /locus_tag="VFQ61_31145"
     CDS             653..1024
                     /locus_tag="VFQ61_31145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959003.1"
                     /translation="MQGKNRGQERRRHRVFVTRNTEYHFRDEVCVAVKDVKSGMWLQQ
                     HQALRRKVTSGVRFFAQGGAAPTSFPPNVGESLYFGEEDGERELITSALCSHHRPSKS
                     DIDMYEEQSGEPDVWAEAFAS"
     gene            1228..1410
                     /locus_tag="VFQ61_31150"
     CDS             1228..1410
                     /locus_tag="VFQ61_31150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959004.1"
                     /translation="MSQTVEILPRVKDHRAVEILAKNIYREFRAGGFSERDVLALAGE
                     LLGLVTRDVRGDGAER"
     gene            2280..3977
                     /locus_tag="VFQ61_31155"
     CDS             2280..3977
                     /locus_tag="VFQ61_31155"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9959005.1"
                     /translation="MIHEKGGTERREVFEGAEISVGRVQGNELVLPKGNVSKRHARLL
                     FREGRFIVTDLNSTNGTYVNRRRITQATIVREGDRIYIGDFILRIEAGGAPAGEGSVA
                     GPLPGGVTAPVRKAQVTSESQSGANLRSMADEEEDLTRSPVRPPRSSPSASAPAVISA
                     RGTGPDSALMSAVGSVVQRVARAFPRAELEADVPDTLGERVEARLREVLNETQKERSL
                     NDSLLERVLVLARAELLELGPLDDLLVDPAVSDIALLGHERLTFTRANVAHVAEVGFS
                     CEAAVRFACARLCSRSGQPLGQAARVERMLPDGTSFSASLLSGNGTVLLFTRRARAPG
                     SLDELVRRGTVSRVVASFLQQCLQARLNLLVIGPRDGGLETLLGAFEESLPEGEAVYH
                     CRTALAADNESMERVLEGAALAARAPLSRVFIEAHADGAAELVVGSVCDGADGLIVAR
                     TATSLRRGLLRILAELRPRFASGAAELLAGAFDLVIEVARLRDNRHRVLRVAEVLGSS
                     DGEIELSDVFTFVIDRTAAGGMIEGSFLPAATLPAVADVMRLRGISLDSSVFRSPAR"
     gene            complement(4131..4643)
                     /gene="def"
                     /locus_tag="VFQ61_31160"
     CDS             complement(4131..4643)
                     /gene="def"
                     /locus_tag="VFQ61_31160"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET9959006.1"
                     /translation="MIREILKYPDPRLREPAKPVTAVTDEVRQLLDDMAETMYAAQGC
                     GLAAPQIGVDMRIFVVDCAGEDEPSDFRVFINPEIIATDGSQTWNEGCLSFPGVNEEI
                     KRAERVTMRALDRAGQTFEIEADGLLAVALQHELDHLNGVLMIDKLNAIKRRMMGRKL
                     ARAKQGQAEA"
     gene            complement(4707..>5109)
                     /locus_tag="VFQ61_31165"
     CDS             complement(4707..>5109)
                     /locus_tag="VFQ61_31165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9959007.1"
                     /translation="ATTPQAPRASIDALEALGVRVIRVPATAEGRCDTIALLKELAAR
                     EVVNVMCEGGSELAGSLLANGLVDELHVFMAPRLLGPRGRPGAVDWAGPDNPAEAPRI
                     DPPRWELCGTDAYVWGPLVYPKRAVRTVALG"
BASE COUNT          905 a         1600 c         1630 g          974 t
ORIGIN      
        1 cacggccgca atacgatttc acgacgctcg tcgtgcgcag gagccattgc gccacgcacg
       61 tcttcagccc ctagatcggc ggcgaacgcg cggctcgagg acgcgcgctt ccacatcgat
      121 gtctatcagc actccgtctt cgccgagcct taccaatagc tccgccttca tgcgtccgat
      181 cggcgacccg gctcgaactt gcgcctccac tcgtgcgatg cgcactgcgc gcagacgagc
      241 atgaggatca cgcgccagca acgcgcgtac gcgcgccgct tccacgtcgg gaatgagtcc
      301 acccctggag gtgattttga gcaaaatgct gctgaagccg tagtagattg cggtctctgc
      361 ctcgagccgc gtcttgcctt cactgatcac gtcggcacgt tcgaacagtt cccggattcg
      421 gtaatcactg ctcttgatca acgagatgga acgctcagcg acaagcggct cggtgcgcgt
      481 cttcgcgagt cctggctttc gattctgttt gggggtcaaa cgccaccgtg taccgagcgt
      541 ctgcgtagcc taggccccgg agcaaccgca cactagtttg tggagtgctc cgccataaac
      601 ttttccgagc ccgacgacgc gtggcctaat cgtccctgcg aaaggagctg tcatgcaggg
      661 caaaaatcga ggccaggagc gacgtcggca ccgagttttc gttacgcgca acaccgagta
      721 tcacttccgc gacgaggtgt gcgtggccgt gaaggacgtg aaatcgggca tgtggttgca
      781 gcagcatcaa gcgctacgtc ggaaagttac atccggagtt cgcttcttcg ctcaaggggg
      841 cgctgcgccc acgagctttc ccccgaacgt tggcgagtct ctctacttcg gagaggagga
      901 tggagagcgc gagctcatca cgagcgcgct ttgctcacac catcggcctt ccaagtccga
      961 catcgatatg tacgaagaac aatccggcga gcctgacgtc tgggcggaag ctttcgcgag
     1021 ctaaggcatc agctggtgaa gcccattttc cggttaaacc agttttccat cgtgcgcgta
     1081 aatgtctgcg ctttcgacgg ggtgttcgcg ctgcactgcg aattgcgtta cgcgatctga
     1141 gcgctctaaa cccacaaaat cgctgaaaac ctatctggaa cgcttttcgc tttcagccgc
     1201 ccgggcactg cccaggacat tacagtcatg tcacaaactg tcgaaatact gccgcgagtc
     1261 aaggatcatc gagccgtcga aatcctggcg aaaaacatct accgcgagtt tcgggctgga
     1321 ggcttctccg agcgggatgt tctggcgctg gccggcgagc tattggggct cgtgacccgc
     1381 gatgttcgcg gggacggcgc cgaacgctga gcttacgtcg ctcgtcgata tagaaggacc
     1441 tcagattgcc ctcgcgctac gccgggcggg aacgggagct tgggtaccga cgagctcccg
     1501 gggagttccg tggttctccc agggagctcc agacacctga ggtcgtccgg accaaggggc
     1561 ttccgggcac ttaaggctcc gatacttaag tcctccggac gcttaagtcc tccggacacc
     1621 aaacactttc cgagaggttc tctggacaac gtcctccgga caagcgtcct cgaggaatag
     1681 aagcatcttc gggtgaagcg tcttagccgg gctccgggca caggaacgtg gaactcacag
     1741 gtttctcgcg gttcatctcg tcaccgcggg aacgatcgta aagtaagggc gtctctgcgg
     1801 cgagggtacg cggcgagggc attgctgtcg gggaacgggc gagcagctgc agaggtgaag
     1861 ccgtgccggg ggaaaagtag gcggcgggga tgtaaagagc cgccggggcg agccaagctg
     1921 atgccgggac ggcagaatac ttgctggctg cgcctccgat cctcggtggg aggcggattc
     1981 caagcggcgc gcctagagcc gtgcccgggc cgtcaccgac gtgtcgacca aaaaaatgcg
     2041 ggccgacggc ttcagctgac caagacagct gtcggagcag actttcgttg acataccccc
     2101 gtgcggttcg gaaagctaag gggtgagcca cgctgcggac cgcttcatcg cagtgagagc
     2161 tgcagcggtg gctggcgcaa tcgtcgccag tacctctctg ctcgcggcct gctaatcatt
     2221 cctcaatacc tgccgtggtt tcgccgactt ctccagccaa ggctcccgtc ttctccatcg
     2281 tgattcacga aaaggggggc acggagcgca gagaagtatt cgaaggggcg gaaatcagcg
     2341 tgggccgcgt gcagggcaat gagctcgtgt tgcccaaagg caacgtttcg aagcgccatg
     2401 cccgcctgct gtttcgagag gggcgcttca tcgtcaccga tttgaacagc acgaacggca
     2461 cgtacgtgaa ccgccgtcgc atcacgcagg ccacgatcgt gcgtgagggc gatcgaatct
     2521 acatcggcga tttcattctg cgcatcgaag cgggcggagc accggcaggg gaaggctcgg
     2581 tcgccggccc tctaccgggc ggcgtaaccg cgccggtgcg caaggcgcag gtgacgtccg
     2641 agtcgcagtc gggcgcgaac ctgcgctcga tggcggacga agaggaggac ctaacgcggt
     2701 ccccggtgcg gcccccgcgc tcgagcccgt cggcgtcggc gccggctgtg atttctgcgc
     2761 gaggtaccgg tcccgactcg gcgttgatga gcgccgtcgg gagcgtcgtg caacgggtcg
     2821 ctcgagcatt cccgcgagcg gagttggagg ccgacgtacc ggacacactc ggcgagcgcg
     2881 tcgaagctcg gcttcgagag gttctgaacg agacgcaaaa agaacgcagc ctcaacgatt
     2941 ctttgctcga gcgggtgctc gtgctcgcgc gagcggagct gctcgaactt gggccactcg
     3001 acgacctgct cgtcgatcca gccgtcagcg acattgctct gttaggtcac gagcggctca
     3061 ctttcacacg cgccaatgtc gcgcacgtgg cggaggttgg cttctcttgc gaggcggcgg
     3121 tgcgctttgc ttgcgcgcgt ctttgcagcc gctcgggcca gcctctcggg caagcggccc
     3181 gggtcgagcg catgctgccg gatgggacga gcttttcggc ctcgcttctg tctggcaacg
     3241 gcaccgtgct gctgttcact cgtcgagctc gcgcccccgg atctctggac gagctcgttc
     3301 gacgcggcac cgtctctcga gtggttgcct ccttcctgca acaatgcctg caggcgcgcc
     3361 tgaatttgct ggtgattggg ccgcgcgatg gcggactcga gacgctgctc ggcgctttcg
     3421 aggagtcgct ccccgaaggg gaagcggttt accactgtcg cactgctttg gcggccgaca
     3481 acgaatccat ggagcgagtg ctggaagggg ccgcgctcgc ggcccgcgca cctctctccc
     3541 gcgtgttcat cgaagctcac gccgacggtg cggctgaact cgtcgtgggt agcgtgtgcg
     3601 atggcgcgga cgggctcatc gttgcgcgca ccgcgacctc gttacggcgc ggattgttgc
     3661 gcattctcgc ggagctgagg ccgcgctttg cgagtggcgc ggccgagctt ttggcgggtg
     3721 ccttcgattt ggtgatcgag gtcgcgcgct tgcgggacaa ccgtcaccgg gtgctgcgtg
     3781 tcgcagaagt gctcggctct tccgatggcg aaatcgaact gtctgacgta ttcacgttcg
     3841 taatcgatcg caccgcggcg gggggcatga tcgagggcag cttcttgccc gctgcaacac
     3901 taccagcggt tgcagacgtg atgcgtttgc gcggcatctc tctggacagc tcggtgttcc
     3961 gctcgcccgc gcgttgattc tcgcggcagg tcgcaggcac gccgtcatgg cgcggatcga
     4021 acatgaatcg cgcgccgcga ctcacacggc gcgcagtcaa cgcagcccgc gccggctcac
     4081 cggcggaagc tgagtcggcg tcgatgcaga ctcagctcac ccggactgaa tcaagcttcc
     4141 gcttggcctt gcttggcgcg cgcgagcttt cgacccatca tgcggcgctt gatcgcgttc
     4201 agcttgtcga tcatgagcac gccattcagg tggtccagct cgtgttggag cgccaccgcg
     4261 agcaagccgt ccgcttcgat ctcgaacgtt tggcctgcgc gatccagggc ccgcatcgtg
     4321 acccgctctg cgcgcttgat ctcttcgttt acgcccggga aggacaggca gccttcgttc
     4381 caagtttggc ttccgtcggt cgcgatgatt tcgggattga tgaacacccg aaaatcactc
     4441 ggctcgtctt ctcccgcgca gtccaccacg aagatgcgca tgtccacgcc aatctgaggc
     4501 gcggccaagc cacaaccctg cgcggcgtac atcgtctccg ccatgtcatc gagcagctga
     4561 cgaacttcgt cagtcacggc cgtgacaggt ttggcgggct cacgcaggcg cgggtcggga
     4621 tactttagaa tttcgcgaat catggcagag ctcagggcag ccccaaatct aacaccggcg
     4681 ttccgcgccg caatggccgg ccgtgtctac cccagcgcaa ccgttcgcac cgcgcgcttt
     4741 gggtacacga gcgggcccca gacgtaagcg tccgtgccgc acagctccca gcggggtgga
     4801 tcgatacgcg gggcttccgc ggggttatcc ggacccgccc aatccacggc accgggacgc
     4861 cctcgcggcc cgagcagccg cggagccatg aagacatgga gctcgtccac caagccgttc
     4921 gcgagcaagc tgccagcgag ctccgagccg ccttcgcaca tcacgttgac cacctcacgt
     4981 gcggcgagtt ctttgagcag agcaatcgtg tcgcagcgcc cctcggccgt agctggaact
     5041 cgaatgacgc gcactccgag cgcttccaga gcgtcgatgc tggcccgtgg ggcttgtggg
     5101 gtggtggca
//
LOCUS       DASTKU010000886         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1065997, whole genome shotgun sequence.
ACCESSION   DASTKU010000886 DASTKU010000000
VERSION     DASTKU010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065997"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="VFQ61_31170"
     CDS             complement(<1..1409)
                     /locus_tag="VFQ61_31170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959008.1"
                     /translation="MDLPKFPNTEVRSIDSWTNYTGTIASRKIPIYCLPAGGTDSSVL
                     KLHGDALRAILQYCFDNRATLRVFGSAWSFSKVLEPGNVVLDPGNMNYICQVPPYLLT
                     DAYRARAQHGFVPMFVEGGTQVADINRRLGNDVRLALQTSGAGNGHRIAGAIATGTHG
                     SALGIGALHDTVRALYLMVAPDRALFVQPASLPLCTEGVKAWLGNQTGVTTEVVTDDT
                     LFNAALVSLGSLGVVFGVVIEAVPLYQLFFSRVARRFDDPQLWSVMQTLDTTPLHQVP
                     ATPYHFDVVMHPYPPSGAEPAMFGTLMWKVPAPPGPVSSPLPAIPLASSDLMGFVSKL
                     TQTLVGHLLEPLAMPILRDYISQQLGSNASPSSGQRFPGEIFGPTSLPPGTGASTEIV
                     VDHGYTERALRVVYQTLGDRAPSGELLLGCIGVRFVPGSSALLGMNIAPMNTYIELPS
                     IRNAKVLSLYTAIWDALEAA"
     gene            complement(1411..1917)
                     /locus_tag="VFQ61_31175"
     CDS             complement(1411..1917)
                     /locus_tag="VFQ61_31175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959009.1"
                     /translation="MHLRSGIKESLDEMAMMNQGALRSLRVLVIVFTLVASACCHHDV
                     RLEFPESDAVAGIYECSGTTGPQNCQPSTVTDPAAQNSEGTVFVMMPAECKKQFHRIV
                     IQDAGSASPRVRVECAPLEAPIPALTPIPPPAAPAAPGAPAVTAPSTTPAGAANSAAT
                     TSPAAASH"
     gene            2241..2864
                     /locus_tag="VFQ61_31180"
     CDS             2241..2864
                     /locus_tag="VFQ61_31180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959010.1"
                     /translation="MVKTTAAFAVLAIGSGALLAAAGLVETTLGVVLASPVWGAWGAW
                     GATLVLTNELSTVVLGAFDDLVALVQRTAASGIHAAALKLAGTPTGIRVSALPEVFKR
                     WRREALGDPTERGLPGRVAGWVGNWMLEGVQKRAIRQLGRDAQKQGATEVSLASLSSA
                     LPAIATSALTGPLRRSIALVRWASGAAMFCSLLVTALALFGASWVKR"
BASE COUNT          543 a          958 c         1013 g          565 t
ORIGIN      
        1 gcggcctcga gggcgtccca aatcgccgtg tagagcgaga gcaccttcgc gttacgaatg
       61 cttgggagct cgatgtacgt attcatcggc gcgatgttca tcccgagcag cgccgacgaa
      121 cccggcacga aacgaacgcc gatgcagcca agcaagagct cgccgctcgg cgcgcggtcg
      181 cccagcgtct ggtacacgac gcgcagcgct ctctcggtgt agccgtgatc cactacgatc
      241 tcggtgctcg cgccggttcc aggtgggagc gaggttggcc cgaaaatctc gccggggaag
      301 cgctgaccgc tcgacggcga tgcattcgaa cccagctgct ggctgatgta gtcgcgcaag
      361 atcggcatcg ccagcggctc gagcaaatgc ccgacgagtg tctgggtcag cttgctcacg
      421 aagcccatca gatccgacga tgcgagcggg atcgcgggca gtggtgacga gaccgggccc
      481 ggcggcgctg gaaccttcca catgagcgtt ccgaacatag cgggttcggc gccgctcggc
      541 ggatacggat gcatcaccac gtcgaagtgg tagggcgttg ccggaacctg gtgcagcggc
      601 gtcgtgtcga gcgtttgcat caccgaccag agctgcggat cgtcgaagcg gcgcgccacg
      661 cgcgaaaaaa agagctgata gagcggtacc gcttcgatca cgaccccgaa gacgaccccc
      721 agcgaaccca gtgaaaccaa cgcggcgttg aacagcgtgt catcggtcac cacctccgtg
      781 gtcacgcccg tttggttacc gagccaggcc ttgacaccct cggtgcagag cggcagcgac
      841 gctggctgca cgaacaatgc tcggtccggc gccaccatga ggtacagcgc ccgcaccgtg
      901 tcgtgcagcg cgccgatgcc gagcgccgaa ccatgtgtgc cagtggcgat ggcgcccgcg
      961 atgcggtgcc cattgccggc gccggaggtt tggagtgcca gccgcacatc gttcccgagt
     1021 cgacggttga tgtccgccac ctgcgtgccg ccctcgacga acatgggcac gaagccgtgc
     1081 tgcgcgcgtg cccgataggc atccgtgagc aggtaaggcg gcacctggca gatgtagttc
     1141 atgttgccag ggtcgaggac cacgttgcct ggttcgagca ccttggaaaa agaccacgcg
     1201 ctaccgaaca cgcggagcgt cgcccggttg tcgaaacaat actgcagaat ggcgcgcagc
     1261 gcgtcgccgt ggagcttgag gaccgagcta tccgtcccgc ccgcaggcaa gcagtaaatg
     1321 ggaatctttc gagacgctat cgtgccggtg taattggtcc agctgtcgat actgcgaact
     1381 tcggtgttgg ggaacttcgg taggtccatc tcagtggctc gcggcagcgg gtgaagtcgt
     1441 ggccgccgag ttcgcggcac cggctggggt agtcgagggt gcggtcacgg ccggcgcacc
     1501 cggtgcggcg ggcgcggcgg gcggcggaat cggtgtcaac gcggggatcg gagcctcgag
     1561 cggcgcacac tcgacgcgta ctctcggcga agcggaccct gcgtcttgga tcacgattcg
     1621 atgaaactgc ttcttgcact cggccggcat cattacgaag accgtcccct cgctgttctg
     1681 ggcagcaggg tccgtcactg tgctcggctg acaattctgc ggtccagtcg tgcctgagca
     1741 ctcgtaaatg cccgccacgg cgtcgctctc aggaaactcc aggcgaacgt catggtgaca
     1801 acaagcgctc gcaaccaggg tgaacacaat cacgagcacg cggagacttc gtaatgcgcc
     1861 ttggttcatc atggccattt cgtccagact ctccttgatg ccgctacgca agtgcattcg
     1921 aaaatagtca tttgcgcggc tgaacactat cggccgaatc tgggtgcccg ttgcgtatca
     1981 atcgtgtttt ccgccaatcc gcgccgaaaa gaagttacta tgtcgatagt gcgtaggctc
     2041 ggccctgcgc gagacgaggg tcgtcgttag cacgcgtttg gaagagggat gtcccaagtc
     2101 ccgccctgct gcgagtcaag atcgtgcggc tgcgaagcaa ggcctcgtca cttcgctcct
     2161 cgaaaggctt cagccttctc cggtgctcac tcctcgggcg tgtttcacgc tcgcgcttca
     2221 aggacgcgcg gctgcgggtc gtggtgaaga ccaccgctgc cttcgcggtg ctcgccatcg
     2281 gttcgggggc gctgctcgcc gcggccggtc tagtcgagac cacgctcggt gtggtgctcg
     2341 cttctcctgt gtggggggcc tggggagctt ggggtgcgac gctcgtgctc acgaacgagc
     2401 tctcgaccgt ggtactcggc gcgttcgacg atctcgtcgc gctcgttcaa cgcactgccg
     2461 cgagtggtat ccatgcggcc gcgctcaagc tggcaggcac gcccaccgga attcgggttt
     2521 ccgcgctccc cgaggtgttc aagaggtggc gccgggaggc gctaggcgac cccaccgagc
     2581 gcggcctgcc gggccgggtc gcgggatggg ttggaaattg gatgctcgag ggagttcaga
     2641 agcgcgccat tcgccaactt gggcgcgacg cgcaaaagca gggcgcgact gaagtgtcgc
     2701 tcgcaagcct ctcgagcgcg ttgcctgcca tcgcgacttc cgcgctcacg ggtccgctca
     2761 gacgctcgat cgcgttggtc cggtgggcca gcggagccgc gatgttctgc agcctgttgg
     2821 tgactgcgct cgctctcttc ggtgcgtcct gggtcaagcg ttgatttgat tcgtccagca
     2881 accgtaagaa cgttttaggc tctatcctcg aactcgctcg aggaggtgca gcgcgcagca
     2941 gagatgtatc gctcgcaccc cgatccgatt tcaccaagca ccttcgtaga ggagctgcag
     3001 cacctgctcg actcgggctc aagcgtgagc gatcagggat gatcgatcag cactcgcaaa
     3061 gtgccgtcgc tctggcggc
//
LOCUS       DASTKU010000887         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_173285, whole genome shotgun sequence.
ACCESSION   DASTKU010000887 DASTKU010000000
VERSION     DASTKU010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173285"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..3647)
                     /locus_tag="VFQ61_31185"
     CDS             complement(72..3647)
                     /locus_tag="VFQ61_31185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HET9959011.1"
                     /translation="MKADDSTPNRAGLLLAATDLSTFSDCRRATLLDREAAQGLTARA
                     PYRDDPAMQLLRERGQQHEARYLELLRAKYAEAPFQVPCGVPKSLSDWQRGAALTWAA
                     MKDGHRVIYQPPLCHGQWHGRADFIERVDHGPRELPSLLGTYHYRVLDTKLAQRPRSG
                     AVLQLCVYSELLAKLQGRLPEHMILVSPPGARGDGAPRSVELRTEDFAAYFRHIRCRM
                     EQFTQEGELEAVYPEVREHCDVCAWWSSCNERRRRDDHLSFVAGISRRDQRLLETAGV
                     AKLEQLGKLELPLPARPKKLRAETLERLQQQARLQLEARVNGPRYEVIEPVLPGKGLA
                     ALPAPSAGDVFFDIESDRYAVDGTFVYLFGWVEGALDGGLRFERLWATNRGEERQNFE
                     RFVDRVTDRWREYPDMHVYHFASTEKAILGNLAGRYATREDEVDDLLRHGVLCDLLPV
                     VKQALRAGVESYSLKDLEQFHGFRRETDLREAAAARRSFELARETLRNAELPGVVSTV
                     EAYNREDCYSTRSLRDWLERLRAQLIAAGRAVERPSAIREPESEKLGAWLRRVAALRQ
                     QLLEGMPEDAENRTEGQRARQLLADVLDWHRRKEKPKWREYFRSRTLSAEGCFEESGP
                     IGQLGPEQHLGAHKKSQAYEYSFPPQDYKIKVGDQVECPVTQQEIGEVIEIRREAHAI
                     VVKRTKPLSQPPVALVEKPAHFSVGDLQDALAEIADSLAATGFGSDSPATGDEWGDPV
                     VTFPSARALLLREPPKLSSGVALRLPGEEPAHAAVRVAPHLRQTVLAIQGPPGAGKSY
                     TASRMILALLRRGKRVGITATSHKVIGNLLFKVQEAAEAERLEVFCLQKVSTQDEGLV
                     HSHNKVTTKGGDSFLTERSKLRRQSEGIEPVLAGTPYFWATPAMRQSVDVLIVDEAGQ
                     FSLANALAVSVAADSLVLLGDPRQLAQPDQGSHPPGAGASALEHVLHGEQTLAETQGL
                     FLEHTWRLPPAIVEFTSKHFYSGRLKAHPDCAAQSLEAPISLSRFAGSGIFFEPLEHE
                     GNVSFCPEEADHIVSLIRDFMAGPTCWIDRRGHRHRIELRDILVVAPYNAQVQVIGEA
                     LARADLVNARVGTVDKFQGQEAPIVIYSMTTSSPEDAPRGIDFLFELERLNVATSRAQ
                     AVVIVIASSRLLDVECKTPEQMRLVNGLCGAVEIAHLQHPRQAAE"
     gene            complement(3749..>3931)
                     /locus_tag="VFQ61_31190"
     CDS             complement(3749..>3931)
                     /locus_tag="VFQ61_31190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959012.1"
                     /translation="ARARAFLETLSEWGDGCGDVLVAHAGFVRALEVVCGSGGWEEVM
                     RKRIEYLRWACLRPTT"
BASE COUNT          727 a         1285 c         1163 g          756 t
ORIGIN      
        1 agagtcgcca atgtagaggg ttgttcactg aggaattgcc cctgctggta gattagctgc
       61 ctacgcgacc gtcactccgc cgcctggcgc gggtgttgta gatgcgcgat ttcgaccgct
      121 ccgcacaagc cgttgacgag gcgcatctgt tcgggggttt tgcactcgac gtcgagcagc
      181 cgtgagctgg cgattacgat gacgaccgct tgcgcgcgag aggtcgcgac gttcaagcgc
      241 tctagctcga acaagaagtc gatgcctcgg ggcgcgtcct cgggactcga ggtcgtcatc
      301 gagtagatca caatcggtgc ttcctggccc tgaaacttgt ccacggttcc tacgcgagca
      361 ttgaccaagt ccgcgcgggc cagcgcttcg ccgatcactt gtacttgggc attgtagggc
      421 gcgaccacca ggatatcgcg taactcgatc cggtgtcgat gaccgcgacg atcgatccag
      481 cacgtcggtc cggccatgaa gtcgcggatg agcgacacga tgtgatccgc ttcctccggg
      541 caaaagctga cattcccttc gtgctcgagg ggttcgaaga agatgccgct tccggcaaac
      601 cgactcaaag agattggcgc ctcgagcgac tgagccgcac agtccgggtg cgccttgagg
      661 cgccctgaat agaagtgctt cgacgtgaac tccacgatcg cagggggcag ccgccaagtg
      721 tgctctagaa acaacccctg agtctcggct agagtctgtt ccccgtgcag cacgtgttcg
      781 agcgcggacg ctccggcccc gggcggatgc gagccttggt cgggctgcgc cagttgtcgt
      841 ggatcgccga gcaacactag gctgtccgcc gcaaccgaaa ccgccagcgc gttcgccaac
      901 gagaattgcc cggcttcatc cacgatcagt acatccacac tttgccgcat tgccggcgtg
      961 gcccaaaagt agggtgtgcc ggcgagcacg ggctcgatgc cctcgctttg tcgtcgaagc
     1021 ttgctgcgct ccgtcaagaa cgaatcccca cctttggtgg taaccttgtt atgcgagtgg
     1081 accaagcctt cgtcttgcgt tgacaccttc tgcaggcaaa acacctccaa ccgctcggcc
     1141 tccgcggcct cctgaacttt gaacaaaagg tttccaatca ccttgtgact ggtggccgtg
     1201 atcccgacgc gcttgcctcg gcgaagcagg gccaggatca tgcgactcgc ggtgtagctc
     1261 ttccccgcgc cgggggggcc ctgaatggcg agcaccgttt gcctgaggtg cggggccaca
     1321 cggacggcgg cgtgcgccgg ctcttcaccg ggaagcctca aggcaacgcc ggaagacaac
     1381 ttcggtggct ctcgcagcag cagcgcgcga gcactcggga acgtcaccac cggatcgccc
     1441 cattcatctc ccgtagcggg cgaatcgctc ccgaaacccg tggcggcgag cgagtccgct
     1501 atttctgcaa gagcgtcttg aaggtctccg acgctgaaat gtgcgggctt ctctaccaga
     1561 gccacgggtg gttggctgag cggcttggtc cgtttgacga caatcgcgtg ggcttcacgt
     1621 cgtatctcga tgacctctcc tatctcttgc tgggttaccg gacattcgac ctggtctccg
     1681 actttgattt tgtagtcctg cggaggaaac gagtactcgt atgcctggct cttcttatgg
     1741 gcgccgaggt gctgctctgg gccgagctgg ccaatggggc ctgactcttc gaaacaccct
     1801 tcagcggaca acgtccgcga acggaagtat tcgcgccact tcggcttttc cttccgccga
     1861 tgccagtcga gcacatctgc cagcaactgg cgcgctctct gtccttctgt gcggttctcc
     1921 gcatcctctg gcatgccctc gagcaactgt tgccgcaaag cggccactcg gcgcagccat
     1981 gccccgagct tctcactctc gggctcgcgg atggcactcg gtcgctctac agcgcgcccc
     2041 gcggcgatca gctgcgcccg cagccgctcg agccaatccc gtaggctgcg cgtggagtaa
     2101 cagtcctcgc gattgtaggc ttccaccgtg gagaccactc ctggcagttc ggcgttcctc
     2161 aacgtttcgc gcgccagttc gaatgagcgc ctcgccgccg cagcctctcg caggtcagtc
     2221 tctcgtcgga aaccatggaa ctgttccagg tctttcagtg agtagctctc cacgcccgcg
     2281 cgcaacgcct gcttgacgac cggtaacaag tcacagagca cgccatgtcg aagcaaatcg
     2341 tccacctcat cctcgcgagt tgcgtaccgg cccgcgaggt tgccgagaat tgccttctcg
     2401 gtcgaggcga agtgatacac gtgcatatcc ggatattccc gccaccgatc cgtcacccga
     2461 tctacgaagc gctcgaagtt ttggcgctcc tccccccgat tcgtggccca cagtcgctcg
     2521 aaccggagac ctccgtctag cgctccctcg acccagccaa agagatagac gaatgtgcca
     2581 tctaccgcat aacgatccga ctcgatatcg aagaagacgt ccccagccga aggcgcaggc
     2641 agcgccgcca aacctttacc gggcagaacc ggctcgatta cttcgtagcg gggaccgttc
     2701 acgcgagctt cgagctggag tcgcgcctgc tgctggagcc gctcgagcgt ctccgcgcgc
     2761 agctttttgg gccgcgccgg caacggcagc tcgagcttcc ccagctgctc cagcttggcc
     2821 acgcccgcgg tctcgagcag gcgttgatct cgccgcgaga tcccggctac gaacgacaga
     2881 tggtcatctc gacgtcgccg ctcattgcac gaggaccacc aggcgcaaac gtcgcaatgc
     2941 tccctgacct cgggatatac ggcctcgagc tcaccctctt gggtaaattg ctccatccga
     3001 catcggatgt gtcgaaagta cgccgcgaag tcttcggtac gaagctcgac tgaccgaggg
     3061 gccccgtctc cgcgcgcgcc gggagggctc acgagaatca tgtgctcggg aagccgtccc
     3121 tggagcttcg cgagcaactc cgagtagacg cataactgga gtacggcgcc gcttcgaggc
     3181 cgttgagcaa gcttggtatc tagcacccgg tagtgataag tgccgagcag gctcggcaac
     3241 tctcgaggac cgtggtcgac gcgttcgatg aaatccgcgc gaccgtgcca ttgaccatga
     3301 cagagtggcg gctgatagat aactcgatgc ccgtccttca tagcggccca agtcagagcc
     3361 gcgcctcgtt gccaatcgga cagactcttg ggtacgccac acggcacttg gaacggcgcc
     3421 tcggcatact tggctcgaag cagctcgaga tagcgcgctt catgttgttg cccacgctcg
     3481 cgcagcagct gcatcgccgg atcgtctcga tagggggcgc gagcggtgag cccctgtgcg
     3541 gcttctcgat cgagcagagt cgcgcgacga caatcactga acgtgctgag atcggtggcg
     3601 gcgagcagga gaccagcgcg gtttggggtc gagtcgtcgg ctttcatcgt gctgaattgc
     3661 gcacgagata gcccgccgaa cgcggggccg caacccaaag ccccgcacgg gatctacgtc
     3721 gagcccagct agcctcagag cctgggttct aagtcgtcgg ccgcagacac gcccaacgca
     3781 ggtattcgat acgttttcgc atcacctctt cccagccgcc cgatccgcag acgacttcga
     3841 gggcgcgcac gaagcccgcg tgagcgacga gcacatcccc gcaaccatca ccccattcac
     3901 tcaacgtctc tagaaatgct cgggcacgag c
//
LOCUS       DASTKU010000888         3740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1290934, whole genome shotgun sequence.
ACCESSION   DASTKU010000888 DASTKU010000000
VERSION     DASTKU010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290934"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VFQ61_31195"
     CDS             <1..202
                     /locus_tag="VFQ61_31195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9959013.1"
                     /translation="VPHRAGRKNKTQDGRKLRRYKRRWHVERLFAWLQNFRRLITRFE
                     HKASNFLAFLKLACVIILLRRL"
     gene            complement(211..2733)
                     /locus_tag="VFQ61_31200"
     CDS             complement(211..2733)
                     /locus_tag="VFQ61_31200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959014.1"
                     /translation="MKQRRVEGFVQKLGNFRASVLLFGFSAACSSGAPDLVTRNDGPA
                     PLEVCVNGGELISIGADKDESGTLEDSEVTSHFTVCNGSQGNSPLFGTVDEPAGEHCA
                     GGGKRIVIGFDNGAGGGLAANGTLEDGEITRSDYVCNGDAGAAGPAGADGTNGQDGVD
                     GRDGIDGQNGTDGTDSLPTLVRVVSEPASAEHCPTGGQRIEIGLDSDASGSLSDEEVQ
                     STEFACNGLDGENGAAGEGQLLRTTRIPAGDQCPAGGELLEFGLDNGQFGSTARDEIL
                     QDEEVRSAIPVCDGVSGSRSLVAWLAEPAGERCPGGGRMVLSGQDDGLGGGTAGDGVL
                     QTGEVRAANTVCNGINGKDAITSLVNVTPEPAGANCSGGGQRITSGLDNGAGGSAVAG
                     NGVLETGEVLSTSFVCHGVNGTNGTNGTNGATSLVSVTAQAAGPNCANGGYRVSTGLD
                     DGAGGGKANDGTLQAGEIDSISYVCNGTNGTNGTNGVNGANSLVRVSVETAGVNCTNG
                     GRKIEAGLDDGTPSGTARDGQLQAGEIDTTSYVCNGTNGINGTNGTNGTNGSSSLVKV
                     STESAGPNCTAGGRKVESGLDDGSPTGTARDGILQAGEVESTSYICNGANGTNGTSGT
                     NGYNSLVSLTPVAAGAACTNGGTRVNSGLDNGAGGGTARNGVLEPGEVTSVEYVCDGG
                     GPLNSGILTDANGAALGRVVSATNRSLSIVTSKGYLTTLSWAGVFPAAQIYYTSYAAG
                     VCGGSAYLNAASSSSIQYMYGKNLVWSAAFSSFMTVPASHLMQDGTAISVQPASSGFT
                     IQGWENPTCAAQTSNNYMWPLVTISRSAVGLPASIAPPLTIN"
     gene            complement(3361..>3740)
                     /locus_tag="VFQ61_31205"
     CDS             complement(3361..>3740)
                     /locus_tag="VFQ61_31205"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959015.1"
                     /translation="RLVNTDLDRAIGLFHVACASGNGLGCNDLGVLYTDEARLVRDPV
                     RAASYFELGCSLESADACNNLGVLNERGLGTTQDLDRAHGAYDAGCELGLALACSNAA
                     ALRARDTLQQGRKHSAQFESPTG"
BASE COUNT          624 a         1219 c         1145 g          752 t
ORIGIN      
        1 tgtgccgcac cgagcggggc gcaagaacaa gacgcaggat ggtcgcaagc tccgtcgtta
       61 taagcgacgc tggcacgttg agcgcctctt tgcctggctg cagaactttc gtcgacttat
      121 cacgcgcttc gaacacaaag cttcaaactt cctcgctttt ctaaagctcg cctgcgtaat
      181 cattctattg cggaggttat gagatgggct ctagttaatc gttagcggcg gcgcaatgct
      241 agccgggagc cctacggcgc tgcgtgagat ggtcacgagc ggccacatat agttgttcga
      301 tgtctgcgca gcgcaggtag ggttctccca gccctggatg gtgaagccgg agcttgcggg
      361 ctggaccgaa atcgctgttc cgtcttgcat cagatgtgat gctgggaccg tcatgaagct
      421 actgaacgcg gccgaccaga ctaggttctt cccgtacatg tattggatgg aagaactcga
      481 cgccgcgttc aagtaggcgg agccgccgca tacgccagct gcatagctcg tatagtaaat
      541 ctgagcggcc gggaaaacac ccgcccagct cagggtcgtg agatagcctt tggacgtgac
      601 gatgctcaag gagcggttgg tcgccgagac cacgcggccc agcgcagcac cattcgcgtc
      661 cgtcagaatg ccgctattca gcggcccccc gccgtcacac acgtactcca cggaggtcac
      721 ctcgcccggt tcgaggacgc cgttgcgtgc tgtgcctccg cccgcgccgt tgtcgagccc
      781 gctgttgacc cgcgttcccc cgttcgtgca agcggctccg gccgcaaccg gcgtcaagga
      841 gaccaaggag ttgtaaccgt tggtgccgct cgtgccattg gtaccgttcg cgccgttgca
      901 aatgtaggag gtggattcga cctctccggc ctgcaggatg ccgtcgcggg ccgtgcccgt
      961 gggggaccca tcgtctagtc cggactctac tttgcgccct cccgcagtgc agttcggccc
     1021 ggctgactcg gtactcactt tcacgagcga gctactgccg ttcgtgccgt tggtcccgtt
     1081 agtgccattg atgccattgg tgccattaca gacgtacgag gtggtgtcga tttcacccgc
     1141 ctgcaactgc ccgtcacggg cggtgcccga aggcgtccca tcgtccagcc cggcttcgat
     1201 tttgcggccg ccattcgtgc agttgacgcc cgcagtttca acgctgactc gaaccaagga
     1261 gttggctccg ttgacgccgt tggtgccatt cgtcccattg gtgccgttgc aaacgtagga
     1321 gatggagtca atttcgccgg cctgaagcgt gccatcgttg gcctttcccc ctccggcgcc
     1381 gtcatccaaa cccgtggaca cccgatagcc gccgttcgcg cagttggggc cggcggcttg
     1441 ggccgtgaca ctcaccagcg aagtcgcacc attggtgccg ttggtcccgt tcgtgccatt
     1501 tacgccgtgg cagacgaagg aggtcgacaa cacctctccc gtttcgagta cgccgttgcc
     1561 tgcgaccgcg ctgcctccag cgccgttatc gagacccgag gtgatgcgct gaccaccccc
     1621 gctgcagttg gcgcctgcgg gttcgggcgt cacgttcacc agcgaagtga tggcatcttt
     1681 gccgttgatt ccgttacaaa ccgtattggc ggctctgact tctcccgtct ggagtacgcc
     1741 atcaccagcc gttccgcctc ccaggccgtc atcctgcccg ctgagcacca tacggccgcc
     1801 gcctggacaa cgttccccgg ccggctctgc caaccacgcg accaagctac gcgagccgct
     1861 tactccatcg cacaccggaa ttgcggatcg cacctcttca tcttgcagga tctcgtctct
     1921 cgcagtgctg ccgaattgcc cgttgtcgag cccaaactcc aagagctcac cgccggctgg
     1981 gcactggtcc cccgcgggaa tacgcgtcgt tcggagcagt tgcccttcgc ccgccgcgcc
     2041 attttcgccg tcgagcccgt tgcatgcaaa ctcggtcgat tgcacttcct catcgctcaa
     2101 gctgccgctc gcgtccgagt cgagcccaat ctcgatgcgc tgaccgcccg tggggcagtg
     2161 ctccgcgctg gcgggttccg agaccacacg gacgagggta gggagtgagt ccgtgccgtc
     2221 ggtgccgttt tgcccatcga ttccgtctcg gccatcgacc ccgtcttgtc cgtttgtccc
     2281 gtcggctccg gccgggcccg ctgctccagc gtcgccgttg cagacatagt ccgaccgagt
     2341 gatctcgccg tcctccagcg tgccatttgc ggccaggccc ccgccggcgc cgttgtcgaa
     2401 cccgatcacg atgcgtttcc cgccgcccgc gcagtgctcg ccggcaggct cgtcgacggt
     2461 gccgaatagt ggggaattcc cctggctgcc gttgcagacg gtgaagtggg acgtcacttc
     2521 ggagtcctcc agagtgccgc tctcgtcttt atcggcgccg atggatatga gctctccgcc
     2581 gttgacgcag acttcgaggg gggcgggtcc gtcattcctg gtcacgagat ccggagcacc
     2641 actcgagcaa gccgccgaaa acccgaacag cagcacggac gccctgaaat tcccaagctt
     2701 ttgcacgaag ccctccactc gtctttgctt cacgcgatcg cggtctcgct ctctgaccgt
     2761 cagatcgtct gagtttatgg accgcccggg cggcggagcc gtgagctcgc ttgctccgcg
     2821 acgcttgctc cgcgacatca tgcaacacga gctccgaacg gctgagcgat gcgactaacc
     2881 cacaggcggg tccgccgcgc gcgctctcgg ttttcgagtg tattcggcgg tcgagtgcag
     2941 ctcaagcatt cggtacatcg cgcagcgatc gaggtaggag ttgcgcgtag agccttgcta
     3001 gatgtccgcg tcgcgactcg atcgagcagg agaccgtaga gtctgctcga ccgagccgcg
     3061 cggagttgtg cacatgcttg gggctagagt gttgagccgg accgcgctga atttggacct
     3121 cgctaatccg acatccgaac gagcggtgtt cgggtgagat gctcgggggc tgctcaggag
     3181 ttcggcccga atgcgctgct cgaaagtcca cgctcgagct cgggcgaggc gcgctcaaac
     3241 gcgtaagtcg cgtgggcgct ctcgtcagat taggaaactc accttcctcc tcacaaacgc
     3301 gggcagcgcc agctggcgaa ttcgggcgca agctgacgga atccgattcg gagaaacccg
     3361 ctatccagtt ggagactcga attgggcgct gtgcttccgg ccctgttgca gcgtgtcccg
     3421 agcacggagc gcggccgcgt tggagcaggc cagggctagg ccgagctcac agccggcatc
     3481 gtatgccccg tgtgcgcggt ctagatcctg agttgtcccc aggccgcgct cgttcagcac
     3541 gccgaggttg ttgcaggcgt ccgcggactc gagcgagcaa ccgagttcga agtaggaggc
     3601 cgctcgaaca ggatctcgca ccaggcgcgc ttcgtccgtg tagagaactc cgagatcgtt
     3661 acagccgagt ccgttgccgg aggcacaagc gacgtggaac aggccaatgg cgcgatctag
     3721 gtcggtgttg acgagccggg
//
LOCUS       DASTKU010000889        13894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1514833, whole genome shotgun sequence.
ACCESSION   DASTKU010000889 DASTKU010000000
VERSION     DASTKU010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13894
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514833"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFQ61_31210"
     CDS             complement(<1..359)
                     /locus_tag="VFQ61_31210"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HET9959016.1"
                     /translation="MSPLQTSNPPRELTVAILGTGTGVGKTYFTSALARAMASAHPDA
                     IVAALKPIETGLDSGQVSDADTLTRASVHVQPPAQHPLFGFAPPVSPHLAARQTGSEI
                     QSLAVSDWLRSYQSEVRR"
     gene            complement(356..1576)
                     /locus_tag="VFQ61_31215"
     CDS             complement(356..1576)
                     /locus_tag="VFQ61_31215"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HET9959017.1"
                     /translation="MNLCAAGNDRLAVSSVRRKNGSVNLDVYLERHLKVLAERDLLRD
                     PDECDRRAAVRSRAEELGVSFVDLSSNDYLGLANVSRETCSGLRLGSAASRLIQGTWP
                     EHSALEAEVAEWVGADAALLFTSAFSANLGTISALAGAESVILSDALNHASIVDGCRL
                     ARAQVAVIPHLDLGALETALIRSAQAPVRWVVTESYYSMDGDAPNLPALRALCDRYRA
                     FLVVDEAHALGVFGSEGAGRCAAFGIRPDVLIGAFGKAVGAQGGFVAGTAALRTFLWN
                     RARSFVYSTAPSPELCAQTLNSVRQVRKADDLRAELHARAEQFRNALSRAGVAIAPGG
                     EGPIVAVVVGSSRAALEAARRLQAEGILAHAVRPPTVPEGQARLRLTVTSNFPAREIE
                     RVASAIQRACRLDS"
     gene            2669..3031
                     /locus_tag="VFQ61_31220"
     CDS             2669..3031
                     /locus_tag="VFQ61_31220"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET9959018.1"
                     /translation="MSTPVDLRVGGQNYRVLASTSEAELRRLAELVEERMRALTAPGR
                     QVSPQTLVLAALALAHDLEEERAARRAVEERSREMLSSVLQRLDAILDEPATPVPPAS
                     GSAHKPPDYSEPSPFIED"
     gene            3044..3568
                     /locus_tag="VFQ61_31225"
     CDS             3044..3568
                     /locus_tag="VFQ61_31225"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET9959019.1"
                     /translation="MPPFRAQNPARPSTPPLHVVLVEPEIPPNTGNIARLSAATACPL
                     HLVGRLGFRIDAHAVRRAGLDYWDAVELHQHVDLAAAEANIAARRRASSPFPRTHLFT
                     TRARHSYLDVPVAMGDVLVFGCESRGLPEAILDTRPDNTVGIPLLGDVRSLNLSNAVS
                     IAVFEFLRRLELLR"
     gene            complement(3612..5633)
                     /locus_tag="VFQ61_31230"
     CDS             complement(3612..5633)
                     /locus_tag="VFQ61_31230"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9959020.1"
                     /translation="MFPQIFGKYVLEKELAKGGMARVYLAMLRGAVGFEKRLVVKQIR
                     PELANDPAFVRRFVEEAKTTVELSHPNIVPVYELGVEQGVYYIAMEYCPGVTLSEVLA
                     KTGKLSPEEGAYVGVEICRALDYAHRRAGIVHRDVTPRNVLLDEEGAIRLIDFGIASP
                     VSGAKVGRGEIFGSRGHMPPEQVAGKALSPATDVFAVAVLLVEAWTGKPPFRRATAEE
                     CNEALNTPLVPLAQQDPGLAPVSDIVMRGLALKVSERPAEAEIFSRPLRDFLRNVDSG
                     DVARRLGERVRQAMRTEKPKEAIPSAAPPDGNDRRSRDTHTFAARNEVSQWTRRMQSS
                     PSVRLPLRDNGGSATRKLPSFPDGVEGDGRPSNHLRIGRRDSWGGPATRKLPSIPPEG
                     GEPEGVWEPVDVRPSQQLRVAQPVTRKLPSIPPGGVDSEAVWESVDAGARSSQQLRAA
                     GPATRKLPSFPPDSSSRGTLLPAPPLQLAKGADVVVERPSLSIPPAPPTTILRRAKVM
                     RWLGPTVLGAAGVAWLWKGIEWGSEHGRGLAQASRGVVSERQLAASPEATVASARPEA
                     PVASAPSMVASASASPSASVNAETRAAPGRLRVTADPSAAVSVAGKGASRAGVTPLRD
                     LRLPPGRYTVTLKNKTYGTPIVARVNVESGSDRSVYVDFREAEPRVLIR"
     gene            5632..7584
                     /locus_tag="VFQ61_31235"
     CDS             5632..7584
                     /locus_tag="VFQ61_31235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959021.1"
                     /translation="MARTAQPLLAHGTRFGLRVLLLLAIIHTVACEKDAPTRTVRVRA
                     AVPNGCLNSDPQPTDPLWLAPLGDFSETLSPIQLQLGGPQPLPFPAGTVAIRARVEAA
                     QGELGGIAERALDESTLSILLWPATRTCPLLDSGGFGIGTAIGYSPQSHVLLLAGGDV
                     PGEPAASVAATIFDTAAGRGGRLASESSSAVDGTSLSEPRAFATISPFGSGLLVAGGE
                     NPLRVESELATPSRTAEIFAADQRRFSPGHVQLAVERSRHAALVLASGDTLLIGGRGP
                     NGAALNVLETVSPLTGRSSIAGLAALKAPRLFPAALQLDDGRIFVGGGTASDGRPLGA
                     LEWLSSDAREHLALELPQELPRRYDRVFAALPGGGVLAVGGCQINSETSCRGPCRLGC
                     PPEDGADPDNDPDYDASWITADGQVFSLRIPFAAPQPAILGGSDGMPIVAGGTPSDSK
                     LYRFDPWTVGFSPVLDFGATGPGAASTPLALDPQTHVWFGDAEAGPTLMGARIDVPHR
                     FESPSDLFDASSGKRWLVPDRPAQHHVEFDPAAHQLIFTEDSQVTVFVAGTDYAGLSL
                     LIWAEGGTPEVVLGNQVFGGADCPWPHAHAAEYRLERRAEQVQLYADGSASPPCAVSP
                     GRLRLGVRRGTDAVVVRELRVRREPL"
     gene            complement(7615..9324)
                     /locus_tag="VFQ61_31240"
     CDS             complement(7615..9324)
                     /locus_tag="VFQ61_31240"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET9959022.1"
                     /translation="MSHVRPTATGSLESTPLLNLLVYALDRRLQGTLVLEQTAGKKHA
                     IYFAEGAPAKVKTGQAVALLGDVLHELAIIDRDTRERSYTEAEQQRQLHGQWLLQRGK
                     LEASQLRAALREQLARKVAFLSHLPADTLYGFYADLNLLERFGAPESVRGNSLEIIWR
                     VARELDDLGRAEDVLQRLGVRPVKLHHEAPIARFNFGKEEQVVLEILRAKPQPLNELE
                     ARELLEPERLTRLLYALLITRQLDIGQGTVPLGVDEPPASMRPVAPRRGTPSEGLGAV
                     EPGRGVSSVSLRAQVPSESPEVAAFRAEIVERAGNESESYYDLLGVNRDSAPAEVQAA
                     FLVLAKKWHPDRLPPPLSDLKNAASKVFARMSEASQVLQDGVRRREYDLKIKRAGKEA
                     EEAEHVQRVLRAATAFQKAEVLMKRNNVAAAEHEARQAAQDDPEQADYIALLAWLEAQ
                     KPGANLEASIKSLDRALQIQPNSVRAHWYRGQLYKRLDKMGRALRDFRFVVERDPRHL
                     DAQREIRLYLMRRGDRSTSAPPSTGATPAADGSGRASPAPASGDGKPRSGEGLISRLF
                     KRS"
     gene            9575..10996
                     /locus_tag="VFQ61_31245"
     CDS             9575..10996
                     /locus_tag="VFQ61_31245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9959023.1"
                     /translation="MAPIDLLYAGQRMARAQVLVVDDEPSILSTLKKALSLEGYAVEV
                     AGGIRVAEERLAKKTFDGMLLDVGLPDGDGLDLLSRVKAAHPDLAVIMMSGHGTIETA
                     VRATRLGALDFLEKPLSTDRLLLVLENALRLLRVEAEAQEWKKAGGYFDELLGESRAM
                     QELRQHVLRAARSKASVLITGERGTGKELVARAIHRASPRASAPLERLNCAAVPAELI
                     ESELFGHEAGAFTGATKQRRGRFERASGGTLFLDEVGDMPLAMQAKLLRVLQESEIER
                     VGGNETLRVDVRVVAATNKDLPASARDGAFRADLFDRLNVVPLELPPLRARREDIVLL
                     AEQFLTQARTINGRPQVTFSDDARAALERLPFPGNVRELKNLVERLVILNPDDQITRA
                     DVERFSGAATTPEAVGLYRPGTPFKVLADEAERVILEEALAHHGGQMAATARALGLER
                     SHLYKKAKALGLRGAPSAEEEVG"
     gene            complement(11067..>13894)
                     /locus_tag="VFQ61_31250"
     CDS             complement(11067..>13894)
                     /locus_tag="VFQ61_31250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9959024.1"
                     /translation="AGRAAGIALLVGCGSGCAAGGKPAPVSPASNQSKPKPASPEERL
                     RRGEERLRESEYALAEADLRSALQIAPGRAGIALAELLRITGRLEAAVTAARQARTAD
                     ASYEEKARVIAAQALFDAGHWDEAKAEVEPMATRDKAWLARLQLAEIELAAGKIDRAQ
                     PLLMSLIEAYNDSRIDETDSAALSLVARAAWLLRSHRDANTLFTAAEQAGKADARLLL
                     WRTELFLEKYDPANAEQIVEEVLAKAPKNPEALTLLAEVRLSQALDFAEAERLAREAL
                     AVNPRLARAHFVLAGVALRDMELARAEAQLGEGLRGSPRDLNLLSLQAVVKFLADDAT
                     AYQLAKQRVFELSPKYSRFYSILGEYADWEHRYSEIVEIMKEALTVDDKDPLALAQLG
                     FNLIRSGDEAPGVSALSRAFSLDPYNVRVYNTLELYDTLIPKAYVTSKHARFLIRYHK
                     DDRDVLERYVPALLDRAFDAMREGYGFAPELPIGVELYAERQSFAIRTSGLPQTAIQG
                     VCFGRTLAALSPQQESFNLGMTLWHELSHVFHIQLSRSRVPRWFTEGLAEYETLSTRP
                     EWSRHHDLELFELMRAGRLPQVANMSRAFTRAEAISDVATAYYASSRLVAMLAERVGR
                     AKIAEMLRAWGAGKNTSQVVEAALGMSLVDLDSQFRASLAPMQARYERQFVPVTRARP
                     VSVLEPELSNKPKSLESWVELARAELDQGDVEAAERAAQAALKLKRDEPQAEFVLAQV
                     ARARDNAPLAAQKLSALVARGVDGYAIAMAQAELSTTQSDKAAAAAQYKRALTWDSTQ
                     AEPLLALASMAREAKDTQAELEYLKPLAVLAPHQPAVQLRYVRALLEQGRVAEAVLAG
                     ETAIYSDMLGFETHRAYAQALAKDGKAERARFEYESALLCKSAPERRAELQNEFATLL
                     EQMGQRAAARKQREAAQAATSKPANRD"
BASE COUNT         2426 a         4720 c         4263 g         2485 t
ORIGIN      
        1 cgtcggacct cgctttggta gcttcgcaac cagtccgaga cagcgaggct ctggatttca
       61 ctgccggttt ggcgggcggc gagatgaggt gaaacggggg gagcgaaccc aaagagcgga
      121 tgttgggcgg gtggttgcac gtggacactc gctcgggtga gcgtgtccgc gtcggagact
      181 tggccggaat ctaggcctgt ctcgatcggt ttgagtgcgg cgacgattgc gtcggggtgt
      241 gcggaggcca tcgcacgagc aagcgcgctg gtgaagtacg tcttgcctac gccggttccg
      301 gtaccgagaa ttgctaccgt gagttcgcgt ggggggttgc tcgtttgtag agggctcatg
      361 agtccaatcg gcaagcgcgt tgaatggcgg acgcgactcg ctcgatctcg cgggcgggga
      421 agttgctggt taccgtgagc cgcagtcgag cttggccttc gggaacggtc gggggtcgca
      481 ccgcgtgagc taagattcct tcagcttgta gtcgacgtgc ggcctcgaga gccgcccggc
      541 tggagcccac cacaacggcg acgatcggcc cttcgccgcc cggagcaatc gcgacaccgg
      601 cgcggctcag cgcgttgcga aactgctctg cgcgggcatg cagctccgct cgcaagtcgt
      661 ccgccttccg cacctggcgc accgagttca aagtttgggc gcagagctcg ggtgagggcg
      721 cggttgagta aacgaacgac cgagcccgat tccacaagaa ggtcctcagc gcggcagttc
      781 ctgccacgaa gccgccctga gccccgacgg ccttgccgaa cgcgccgatc agcacatccg
      841 gacggatacc gaacgcggca cagcggcctg cgccttcact tccgaagacg cccagcgcat
      901 gcgcctcgtc gaccaccagg aaagcccggt agcgatcaca cagtgcccgc agggcaggaa
      961 gatttggcgc gtcgccgtcc atgctgtagt agctctccgt cacgacccag cggacaggag
     1021 cctgggcgga tcgaatcagg gcggtctcga gtgcgcccaa gtccagatgg gggatcaccg
     1081 cgacttgagc gcgagcaagg cggcaaccgt cgacgatgga ggcgtggttg agagcatcag
     1141 agaggatcac ggattcggcg ccggcaaggg cggaaatggt cccgaggttt gcggagaacg
     1201 cactcgtgaa gaggagcgcc gcgtcggcgc ccacccactc cgcgacctcg gcttctaacg
     1261 ccgagtgctc gggccaggtg ccctggatca gccttgaagc cgccgatccc agccgcaacc
     1321 ctgaacatgt ttcacgtgaa acgttggcca gtcccaaata atcgttcgag ctgagatcga
     1381 caaaagatac ccctagctcc tcagcccggc tgcgaacagc cgcccggcga tcgcactcgt
     1441 ctgggtcccg gagaaggtcg cgttcagcga gcactttgag gtgccgctcc aaatacacgt
     1501 cgagattcac cgagccgttc ttgcgccgaa ccgagctgac agcaagacgg tcattgccgg
     1561 cggcacacaa gttcatcgcc cgcgcgggcg tatctcgacc caggcacccc tgcggactcg
     1621 gggctcgccg atgcaccgcc ctggcgcatc cctctgccct tagaacgacc gcgaagacgt
     1681 cgctaccggc caccactctg cccgctgcgc ttcgctgcag cggcgaaact ggaggcgccc
     1741 gcacgcgaac acgcgcagcc cccgcctcgg atcgactgcc gttgctcggc ctctcgcgct
     1801 tcgcgcagcc attggccccg tgatcgaaag cacgcgcgtg cggacggcgc agcctccccg
     1861 acttcgcggc ggtgtcgcgg ccgccacgac cgtaggagcg ccggggactc tcccgtcccg
     1921 ggggcttccc agtgaaactc acggctccta aaccacgacc cccgatgaaa gccgagaagc
     1981 ccccgcgggc gccaccacga cggcggagga cagcaaacgg agcccccgct ccgtcgcggc
     2041 tccgattcga cggacgcggg agcgggcgcg tgccgccgca acctggcaac ctgccgcaac
     2101 tgcggccgcg gcgcgattcc atgttggagg ccggaacaac agcttcgtgc gcgaaaatcg
     2161 ccgttgcgag aagggcccgc aaagtccgga tcgaacggtg ccgcggaatg cgagttgtct
     2221 ctctggtgca acctcactcg atccactggt ccacccggcg cgacggcagc cccggctcgc
     2281 cgatgtgcag ctcgcgcgga ggggaggaaa cgaaccgaac aaccccagga ctaacgccac
     2341 tcactcgctc aatgcggcgc tcgaacggcc gcgcatgccc ccggcagagc cgagcagctc
     2401 cgtatgcgac acgctgaccc ggcccgggcc gacattcgga atgagctgtc ccaagtgaca
     2461 gttaatggat cggagctggc tcgccggact cgtggaacca tcgacgctcc acgcttgggt
     2521 tccagatcgc gttccttacc ttgggcacac ctgaacggcg tttcacgtga aacatcggtt
     2581 ccactcccct tagagactcg caaagctcat ctgggagcca gaaacagcca tttccatagc
     2641 tctcacccgg tgctaccgta ccgcgcgaat gagcaccccc gtcgacctcc gcgtaggggg
     2701 ccagaactac cgagttctcg cctcaacgag tgaggccgag cttcgtcgac tcgccgagct
     2761 ggttgaagag cggatgcgag ccctaacggc tcctgggcga caggtctccc cgcagacgtt
     2821 ggtgctggcg gcgctggccc tggctcacga tctcgaggaa gaacgcgccg cccgccgagc
     2881 cgtcgaggaa cgctcccgtg agatgttgag ctcagtgctc cagcgattgg acgccattct
     2941 ggacgagcct gcaacgccgg ttccgcccgc gtctggttcc gctcacaagc cgccggatta
     3001 ctcagagccg tcgccgttca tcgaggattg agcgactgca gtgatgccgc cctttcgagc
     3061 gcaaaatccg gcgcgaccgt cgacgccccc tctgcacgtc gtcctggtcg agcccgagat
     3121 tccccccaac acgggaaaca ttgctcggtt gagtgccgct actgcgtgcc ccctgcacct
     3181 ggtgggacgc ctaggatttc gcatcgacgc ccacgccgtt cgtcgcgctg gcctcgacta
     3241 ttgggacgct gtcgagctgc accaacacgt cgatctagcg gcggcggagg ccaatatcgc
     3301 ggcgcggagg cgagcctcct caccctttcc gcggacgcat ctattcacca cccgtgcccg
     3361 acacagctat ctcgatgtgc cagtcgcgat gggggacgtc ctcgttttcg gttgtgagtc
     3421 ccgcggcttg cccgaagcta ttctcgacac ccgccccgac aacaccgtcg gaatccccct
     3481 cctcggggac gtccgctccc tgaacctttc gaatgcagtc tcaatcgccg tcttcgagtt
     3541 cctgcggcga ctcgagctgc tgcgctaagg cttcgccccg gacgacaacc cgcggcgacg
     3601 ttcgagcggg actaccggat caacacgcgc ggttccgcct cccgaaagtc aacgtaaacg
     3661 ctgcgatcgg atccgctctc gacgttcacg cgggcaacaa tcggggtccc gtaggtcttg
     3721 ttcttcagtg tcaccgtgta acgaccgggc ggcaaacgca ggtcccgcaa aggggtaacc
     3781 ccagcacgcg aggctccctt gccggccaca ctcacggccg ctgagggatc tgctgtgacc
     3841 cgcagcctgc cgggagcggc tctcgtttcc gcgttaacgg aggcacttgg gctggcgctg
     3901 gcgctcgcga ccatcgacgg tgcactcgcg accggagcct ccgggcgcgc tgaagcgaca
     3961 gttgcctctg gggacgccgc gagctgccgt tcgctgacga ctcctcgcga agcctgggcc
     4021 aagccccgcc cgtgctcgct accccactca attcctttcc agagccacgc tactcctgca
     4081 gctccaagaa ctgtcgggcc caaccaacgc ataaccttgg ctcgacgaag aatggtggtg
     4141 ggcggggccg gtggaatgga gagagacggc ctctccacca ccacgtccgc gcccttagcc
     4201 agctgcaagg gaggtgcggg caacagcgtg ccacgtgaac tggagtccgg cgggaatgaa
     4261 ggaagcttcc tagtggcagg gccggctgcg cgcagctgct gcgaggagcg agcgccagcg
     4321 tccaccgact cccaaactgc ctccgaatcc acgccccccg gcggaatcga cggaagcttt
     4381 cgcgtcaccg gttgggccac gcgcagctgt tgcgagggcc gcacgtccac cggttcccat
     4441 acaccctctg gctctccgcc ctcgggcggg atcgacggca gcttcctcgt cgcgggcccg
     4501 ccccaactat cacgccgccc aatgcgcagg tgattcgacg gacgcccatc gccctcaacc
     4561 ccatcgggga aagacggtag tttccgagtc gccgagccgc cgttgtcgcg gagaggcaac
     4621 ctgacgctcg gggatgactg catccggcga gtccattggc tcacctcgtt gcgcgcggcg
     4681 aacgtgtgcg tatcgcggct gcgccgatcg ttcccatcag gcggtgcggc gctgggaatg
     4741 gcctctttgg gcttctccgt tcgcatggcc tgacgcacgc gctcgcccaa gcggcgagca
     4801 acgtccccgg agtccacgtt gcgcaggaaa tctcgaagtg gccgggaaaa gatctcggct
     4861 tcggcaggcc tctcactgac cttcagcgct agtccgcgca tcactatgtc tgaaaccggt
     4921 gccaacccgg gatcctgctg cgccagtggc accaatggag tgttcagggc ctcgttgcac
     4981 tcctcagcag tcgcacgtcg gaagggcggc ttgccagtcc aagcctcgac gagcagaaca
     5041 gccaccgcga acacgtccgt ggcgggggac agagctttcc ccgccacttg ttccggcggc
     5101 atgtgcccgc gcgaaccgaa aatctcgcca cgccccacct tcgctccgga caccggcgac
     5161 gcaatgccga aatcgatgag tcggatggcg ccttcttcat cgagcagcac gttgcgaggc
     5221 gtgacgtccc ggtggacgat ccctgcgcgt cgatgcgcat agtccagcgc acggcagatc
     5281 tcgacaccca cgtaagcgcc ctcttccggg ctcagcttgc ccgtctttgc tagcacctcg
     5341 ctgagcgtga cccccgggca atactccatc gcgatgtagt aaacgccctg ttcgacgccc
     5401 agctcgtaga ccggaacgat gttggggtga ctgagctcga cggtggtctt cgcctcttcg
     5461 acgaaacgcc gcacgaacgc gggatcgttg gcgagctccg gtcgaatctg tttgacaacg
     5521 agtcgcttct cgaagcccac tgcgccacgc agcatggcca gatacactcg cgccatgccc
     5581 cccttggcga gctccttctc gagcacgtac ttgccgaaaa tctgagggaa catggccagg
     5641 acagcgcagc cgcttctcgc acatggtacc cggttcgggc ttcgagtgct gctgctcttg
     5701 gcgattatcc acactgtggc gtgcgaaaag gacgcaccca cacgaacggt gcgagtgcgc
     5761 gccgccgtcc ccaacgggtg cctaaattcg gatccccagc ccacagaccc tctgtggctg
     5821 gcacctctcg gggacttttc ggagactctt agcccaattc agctccagct cggtggtccg
     5881 caacccctgc ccttcccggc gggaaccgtc gcaatcaggg cacgcgtcga agccgcgcaa
     5941 ggagagctcg gcggtatcgc ggagagagcg ctcgacgaaa gcactctctc aattctgcta
     6001 tggcccgcta ctcgcacctg cccgttgctg gacagcgggg gcttcggcat cggcacagcg
     6061 atcggctatt caccccagtc ccacgtgctt ttgctagcgg gaggagacgt tccaggagag
     6121 cccgcagcca gtgttgcggc gacgattttc gacacggccg cgggccgtgg agggcgtctg
     6181 gcttccgagt cctcctctgc tgtcgatggc acgtcgttga gcgagccccg cgcgttcgca
     6241 accatcagtc cgttcggctc tggtctgctc gtcgcgggcg gggaaaatcc gctacgcgtc
     6301 gagagcgagt tggcgacacc cagccgcacc gccgagatct tcgcagcgga ccaacgccgc
     6361 ttctcgcccg gtcacgtgca acttgcggtg gagcgctctc gacacgccgc attggttttg
     6421 gcatccggcg acacgttgct gattgggggc cgggggccga acggcgccgc gctgaacgtg
     6481 ctcgaaaccg tctccccgtt aacggggcgc tctagcatcg caggtctcgc ggcgctgaaa
     6541 gcaccccggc tctttcccgc ggcgctgcag ctggatgacg gccgcatttt cgtcggcggt
     6601 ggtaccgcgt ccgatggtcg tccgctgggc gcgctcgagt ggctttcctc tgacgcgcgc
     6661 gaacacctgg ctctcgagct gccgcaagag ctgcctcgtc gatacgatcg cgtttttgcc
     6721 gcgctgcccg gaggaggggt gctggccgtt ggcggctgcc aaattaacag cgaaaccagc
     6781 tgtcgaggcc cgtgtcgact cggctgcccg ccggaagacg gtgctgaccc cgataacgat
     6841 cccgactacg atgcatcctg gattacggcc gacggccaag tgttctcgct caggatccca
     6901 ttcgcggcgc cccaacctgc catcctcggt ggctccgacg ggatgccgat cgtcgcgggc
     6961 gggaccccct ccgattcgaa actgtaccgc ttcgatccct ggaccgtcgg cttctcaccg
     7021 gtattggact ttggcgcgac ggggcccggt gcagcttcca ccccgttggc gctagatcct
     7081 caaacgcatg tctggttcgg cgacgcggag gctggcccca cattgatggg agcgcgcatc
     7141 gacgttccgc accgcttcga aagcccctcc gatctcttcg atgcctcctc gggcaagcga
     7201 tggttggtcc cagatcgccc agcgcaacac cacgtggaat tcgatccggc cgcacaccaa
     7261 ctcatcttca ctgaggatag ccaagtgacc gtcttcgtgg ccggcactga ctatgctggg
     7321 ctttcgctgc tgatctgggc cgaaggaggc acacccgagg tcgtgctcgg caaccaggtc
     7381 tttggcggag cggattgccc ttggccacat gctcacgccg ctgagtatcg gctcgagcgg
     7441 cgcgccgagc aggttcagct atacgctgac ggctcagctt cgcccccctg cgccgtctcg
     7501 ccgggccgtc tgcggcttgg ggtgcgtcgc ggcaccgacg ccgtcgtggt gcgagaactc
     7561 cgcgttcgtc gtgagccgct ctaaacactg acggctcgac ggcagcgctc gcatttagct
     7621 gcgcttgaac agacggctaa tcaggccctc tccggagcgc ggcttaccgt ctccagaagc
     7681 tggcgccggg ctcgctcgcc cggatccgtc agccgcggga gtggcacccg tggacggcgg
     7741 cgcactcgtc gaacgatccc cccggcgcat caaatacagc cggatctcgc gttgcgcgtc
     7801 caggtgccga ggatcccgct cgaccacgaa gcggaagtct ctcagcgcac gccccatctt
     7861 gtccaaccgc ttgtaaagct gcccgcgata ccaatgggcc cgtacgctgt tcggctgaat
     7921 ctgtagcgcg cggtctaggc tcttgatgga cgcttcgaga tttgcgccgg gcttctgcgc
     7981 ctccagccag gccagcagcg cgatgtagtc tgcctgttcg gggtcatcct gggcggcttg
     8041 ccgcgcctcg tgctctgcgg cggctacgtt gttgcgcttc atcagaactt cggccttttg
     8101 aaaggccgtt gccgcgcgca acacgcgctg aacgtgctcc gcttcttccg cctctttgcc
     8161 agcgcgcttg atcttcaggt cgtactcgcg acgacgcaca ccatcctgca gcacttggct
     8221 tgcttcactc atacgcgcga acaccttgct ggcggcgttc ttcaagtcgc tgaggggtgg
     8281 cggcaggcga tccggatgcc acttcttcgc cagcacgagg aatgccgcct gcacttcggc
     8341 aggagccgaa tcgcggttga cgccgagcag gtcgtagtaa ctctccgact cgttgcccgc
     8401 tctctccacg atctcagcac gaaaggccgc cacctcggga ctttcgctcg gaacctgcgc
     8461 ccgcagggag acgctcgata ctccgcgccc gggctccacg gctcccaagc cctcgctcgg
     8521 tgtgccccga cgcggcgcga ccgggcgcat ggaggcagga ggctcatcca cccccaaagg
     8581 gaccgtccct tggccgatgt ccaactgccg tgtgatgagc aaggcgtaca agagacgcgt
     8641 cagccgctcg ggctcgagta actcgcgcgc ttcgagctcg ttcagcggct gcggcttggc
     8701 ccgcaaaatc tccaggacaa cctgttcctc tttgccgaaa ttgaagcgcg cgatcggcgc
     8761 ttcgtggtga agcttcaccg gccgtacccc gagccgctgc aacacatcct ccgcgcgccc
     8821 caaatcgtct agctctcgcg cgacgcgcca gatgatctcg agtgagttgc cgcgaacgct
     8881 ctccggcgcc ccaaagcgct ccagtaggtt cagatcagcg tagaacccgt acagggtatc
     8941 ggcgggaaga tggcttagaa acgcgacctt acgcgctagt tgttcgcgca acgcggctct
     9001 cagttgggaa gcttcgagct tgccccgttg caacagccat tgaccgtgaa gctgacgttg
     9061 ctgctctgcc tccgtgtagc tccgttctcg cgtgtcacgg tcgataatcg caagctcgtg
     9121 cagcacgtcc ccgagcagcg caaccgcttg ccccgtcttc accttggccg gcgcgccttc
     9181 cgcaaagtat attgcgtgct tctttcctgc ggtttgctcc agaaccaatg tcccctgcag
     9241 acgacggtcc aaagcgtaaa ccaacaggtt gagcaaagga gtcgactcga gcgagccggt
     9301 agcggtgggc ctgacgtgac tcattccagc tggcaaatgc agcctagcac tcctgcagcg
     9361 gaaacagggt agtcaaactt cctacagtaa ggcctttgag ctcgtctgtc gaagaaactc
     9421 ccgcagttgc cgcttttcgc cagtctttcg cgagtgggtt tcccgtcgtg ccgatcacgc
     9481 tgattcggca tctggggaat tcgaaccgcg ggctcaccgc ggaaagactg atgaccaggc
     9541 tcgaacccgc aacgcgagat cactgcgcaa gaacttggcc cccatcgatc tgctctatgc
     9601 tgggcaacgc atggcacgag cgcaggtctt ggttgtggac gacgagccct cgatattgag
     9661 cacgttgaaa aaggcgctca gcctcgaggg ttatgcagtc gaggtcgcgg ggggaattcg
     9721 tgtcgccgag gagcgactgg ccaagaagac cttcgatggc atgttgctcg acgttgggct
     9781 gccggacggc gacggactcg atctgctctc gagggtcaaa gccgcgcatc ccgacctcgc
     9841 cgtgatcatg atgagcgggc acggcacgat cgagacggcc gtgcgcgcaa cccggcttgg
     9901 tgccttggac ttcctcgaaa agccgctctc caccgaccgc ctattgctcg tactggagaa
     9961 cgcgctgcgg ttgctcaggg tcgaggccga ggcccaagaa tggaagaagg ccggaggcta
    10021 tttcgatgaa ctgctgggtg agagccgcgc catgcaggaa cttcggcagc acgtgctgcg
    10081 ggccgcccgc tccaaagcca gcgtcttgat taccggtgaa cgtggcaccg gtaaggagct
    10141 cgtagcgcgt gccattcacc gcgcctcccc ccgcgcctca gcgccgctcg aacgcttgaa
    10201 ttgcgcagcg gttcccgccg agttgatcga aagcgagctg tttggccacg aagcgggcgc
    10261 ctttaccggc gcgacgaagc agcgacgggg ccgcttcgag cgcgcgagtg gggggactct
    10321 cttcctggat gaggtggggg acatgccact cgcgatgcag gccaagctct tgcgcgtgct
    10381 ccaagaaagc gagatcgagc gcgtcggcgg caacgaaacg ctgcgcgtag acgtgcgcgt
    10441 ggtggccgcg accaacaaag acttgccggc ctcggcgcgt gacggcgcgt ttcgggccga
    10501 cctcttcgat cgtttgaacg tcgttccgct cgagcttccg ccgctgcggg cccgacgcga
    10561 ggacatcgtc ctgctcgcgg agcagtttct aacccaggcg cgcaccatca atggtcgacc
    10621 ccaagtcacc ttctccgacg acgctcgagc cgccctggag cgcttgccat tccccggcaa
    10681 tgttcgggag ctgaagaacc tcgtcgagcg cttggtcatc ctgaaccccg acgatcagat
    10741 cacgcgcgca gacgtggaac gcttttccgg cgcagccacg acccccgaag ccgtcggtct
    10801 ctaccgaccc ggcaccccat tcaaagtgct cgccgatgaa gccgagcgcg tcatcttgga
    10861 agaagcgctc gctcaccacg gtggacaaat ggccgccacg gcgcgagccc tcggcctcga
    10921 acgcagtcac ctgtacaaaa aggccaaggc cctcgggttg cgaggcgcac cgagtgccga
    10981 agaagaagtg ggctagcgca ccgaactgtg cccgcagcga aagcgccggg cggcgaggcg
    11041 agcgaccgct cgcagcccgc tctgctctag tcccgattgg cgggcttcga agtggcggct
    11101 tgagcggcct cgcgctgctt gcgcgcggcg gcacgctgtc ccatttgctc gagcagagtg
    11161 gcgaactcgt tctggagctc ggcgcgccgc tcgggagcac tcttacacaa cagcgcggat
    11221 tcgtattcga agcgtgctcg ttcggctttg ccgtcctttg ctagagcttg cgcataggcc
    11281 cggtgggtct cgaagcccaa catgtccgag tagatcgccg tctcacccgc cagcactgcc
    11341 tcggcgaccc tcccctgctc gagcagcgcc cgcacgtatc gaagctgcac cgcaggctga
    11401 tgaggagcca gcactgcgag cggctttagg tactcgagct ccgcctgcgt gtctttcgcc
    11461 tcgcgagcca tgctcgccag cgccagcaac ggctctgctt gcgtcgaatc ccaagtcaga
    11521 gcccgcttgt actgcgccgc cgccgcggcc ttgtctgatt gggtggtgct caactcggcc
    11581 tgggccatgg cgatcgcgta gccatccacg ccacgcgcca ccagcgccga caacttctgg
    11641 gccgcgaggg gcgcgttgtc ccgtgcgcgc gccacctgcg cgagcacgaa ctctgcctgc
    11701 ggctcatcgc gcttcaactt cagcgccgcc tgcgctgctc gctctgcggc ctcaacatct
    11761 ccctgatcca gctcggccct cgccagctcg acccagcttt ccaggctctt cggtttgttc
    11821 gaaagctccg gctctagaac gctcaccggg cgagcgcggg tgacgggcac gaattgccgt
    11881 tcgtagcgcg cttgcatcgg cgccaagctc gcccggaatt gcgaatccag atctacgagg
    11941 ctcatgccca acgcggcctc gacgacttga ctggtgttct tcccggcacc ccatgcgcga
    12001 agcatctccg cgatcttggc gcgaccgaca cgctcggcga gcatcgcgac cagacgactc
    12061 gaggcgtaat atgccgtcgc gacgtcactg atcgcctcgg cccgggtgaa ggcgcggctc
    12121 atattcgcaa cctgtggcag ccgtcctgcc cgcatcaact cgaacaactc gagatcatga
    12181 tggcgagacc actcgggccg ggtactcaac gtctcgtact ccgccagccc ctcggtaaac
    12241 catcggggca ctcggctgcg cgaaagctgg atatgaaaga cgtgcgacag ttcgtgccat
    12301 agagtcattc ccaaattgaa tgactcctgc tgaggactga gcgcggccaa agtcctcccg
    12361 aaacatacgc cttgaatggc ggtctgcggc aatccgctgg ttcggatcgc gaagctctga
    12421 cgctcggcgt acagctccac cccgatcggc aactcgggcg cgaaaccgta accctcgcgc
    12481 atggcgtcga aggcacgatc caatagcgcg ggcacgtaac gctccaatac gtcccgatcg
    12541 tctttgtgat accgaatcag aaaacgagcg tgtttggacg taacgtacgc cttcggaatc
    12601 agcgtatcgt agagctccag cgtattgtag actcgtacgt tgtacgggtc caacgagaaa
    12661 gctcggctga gcgcgctcac gcccggcgct tcgtcgcccg atcgaatcag gttgaacccg
    12721 agttgagcca aggcgagcgg atctttgtcg tcgaccgtca atgcctcctt cataatctcg
    12781 acgatctcgg agtagcgatg ttcccagtcg gcgtactctc cgagtatgga atagaatcga
    12841 gagtacttcg ggctcaactc gaagacacgt tgtttggcga gctggtacgc cgtggcgtca
    12901 tcagccaaga acttcacgac cgcctgtaga ctgagcaggt tcaggtctcg ggggctgccc
    12961 cttagccctt cacccagctg cgcttcggca cgcgccaact ccatatctcg aagggctaca
    13021 cccgcgagta cgaagtgagc ccgtgccagt cgtggattca cggccagcgc ctcgcgcgcc
    13081 aagcgctccg cctccgcgaa gtccagagct tggctcagcc gaacttcggc cagcaacgtc
    13141 agcgcttctg gattcttggg tgccttggcc aacacctctt cgacgatttg ttctgcgttg
    13201 gcgggatcgt acttctccaa aaacagctcg gtccgccaca acagcagtcg cgcgtctgcc
    13261 ttgccggcct gctccgccgc ggtaaatagg gtattggcgt cgcggtgact tcgcagcagc
    13321 caggccgcgc gcgccaccag gctcagcgcg gctgagtccg tctcgtcgat tcgagagtcg
    13381 ttgtacgcct cgatcagcga catgaggagc ggctgcgccc gatcgatttt ccccgccgcg
    13441 agctcgatct ccgccagctg caagcgggcc agccaagcct tgtctcgcgt ggccatgggt
    13501 tcgacttccg ccttcgcctc gtcccagtga cccgcgtcga ataaagcttg cgcggcaatc
    13561 actcgggcct tctcctcata gctcgcgtca gccgtgcgag cttggcgcgc agctgtcacc
    13621 gcggcttcca agcgccccgt gatgcggagc agctccgcta acgcgatccc tgcccgtcca
    13681 ggtgcaatct gaagtgccga cctcaaatcg gcttccgcca gagcgtactc actctcgcgc
    13741 aagcgttcct cgccgcggcg taggcgttct tcgggagagg cgggcttggg tttgctctgg
    13801 ttcgatgctg gcgacacggg cgctggcttc ccgcccgctg cgcagccaga accgcaaccc
    13861 accaagagcg caatgcccgc ggcacggccc gcgc
//
LOCUS       DASTKU010000890        20434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_395813, whole genome shotgun sequence.
ACCESSION   DASTKU010000890 DASTKU010000000
VERSION     DASTKU010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 20434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20434
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395813"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFQ61_31255"
     CDS             <1..187
                     /locus_tag="VFQ61_31255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959025.1"
                     /translation="GPGPLIYEPYPYGGGGNARIQLMGLSDGDIFYVPSEGSPAPLRR
                     VRAIGQKRPPVPAACNL"
     gene            387..6503
                     /locus_tag="VFQ61_31260"
     CDS             387..6503
                     /locus_tag="VFQ61_31260"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9959026.1"
                     /translation="MSSLSLQASADMQVFAHRYLIHFSDTMAYGSHHFLTNFKFQCAA
                     REALLFLAEPDGTEPWRSELGSVELLTREGYTRNLSPLGLGDWAAVLLTAEEVSYGSF
                     RLCFRVVSSAGEPVACGHQQIVVLDRVSRRVSALPEVIQRRLAPIVEPAGATFAERLR
                     RGGSRLAQLFPRELRELSARIARGEFIHESSPYFDIPTRSAPNEAASPDRVAWICGGQ
                     GALDPEWAWRVACALPNSAELLDQADALCRAAWGSDYRSWLSRGGGGGGEGQPRSEQI
                     FKEQLTLYLGAVFTGRILEENGFVPDLILGHSVGEIAAAVLGGALDASAGLTVVATRA
                     SVLLRHCSGGSMLVLQERESSLAPLLSSFSGLTVAVRNHDRQLVVAGPQGTIEQLRGA
                     ARAAGIACKALPGFAFHSSYLLPAVAEFYAQTEPLAFASPRVPVFSPMEQRLIDSRID
                     RPSHWASHLIRTLEFGAAMRTSVGPQNYRFVECGRRLGLGKVLEALGATSVCSAQDLL
                     GEFPAGSVEPSMVDVPSQLAPLAAPEIGTNAEPIAIVAVGCRLPGAKDFDEYRRLLET
                     GECAIADLRELSPELERDFYTNTPGPDKTYTLLSGVVDEPDAEVNVERSKLARLVRAA
                     LAECRVGAVAGRSCVVLGATADGCAEYDEALVLERLYAAEAAGCPSLRAALSREIERI
                     GTSRTLQPASNLARVARELLGETTRTIFVDAACASSLYAIATAARLLAEGECDVAYAG
                     GAFAPSLSNNCLFAQFEGLAQTGSYPLDQRAEGVVFGEGAAFVALRRLSDALASGERV
                     LAVLRGVGLSSDGRSPSASTPRADGQRLAIERAYVASGISPRSVQYVEAHATGTAVGD
                     ATELEALSRFFRAEGVPVGSVRLGSVKSQISHTGWAAGVASLIKVVAALAERNYPAQH
                     HCNEPSTKLEGSPFRLESGVGVNHGGVPWNANIEGQPRRAAVNGFGFGGANAHLIVEE
                     FSREFHQQLIAAARVAPTKQVLRAGVPLEANLRTPDLNSTWPAGALRLLPDVVETLDA
                     TQWTAAVLASQALEPLLGTAGGDRNRIGVVVGMRGKTSTILRANDRIFRDRVLRISTA
                     DSGPLERAAITESVMRWAGSAPSSTPYTLTGGMPNLVSGRVCQLFDLKGPNCVFDAAT
                     DTLESAMLQARAWIAEGTCDWVLAFGIDAVESDHSQARVQLLGEAARVVALALPASEL
                     SAPRAANFEQRTDLAAPPADIQLLRPVLVDLDWRSPGPTSDSEAVVSFSPASLGSEAR
                     VRFDRARYRSDETIIDAQLPTNEPTRVLLVLDLAGDPLSLVQPGAVRERDVLAPLWAF
                     AREHYASLEQNRMAWGVVCLNAWVDSNTPRPETGLVGGFMKSLARELPQARLIAVHTD
                     AALDAGLSCASEGLLGATDEREVFVLDGRKRVHALSPVEFETGGPPQLRADSVVLATG
                     GARGITAELVAAILQRFGCRVALLGRSDPELVPPHLRGASAQTLEAAQSEFVRGAVAS
                     GSSPRQAKAAFAELAAAHEVLQNLSRFQKLPGEVSYLRCDLTEPEDVARSINAVAQRW
                     GTVSFVLHGAGVQTSSKLNRKTPEAFWAVLDVKIAGLRNLLSALQTQFPDHCPQLHVA
                     TSAFSYFGNDGQQDYGAANEALNRLADCASALGQRWSTLGWLAWDGVGMTRGSEYRAL
                     ARAREMRALSAEEGQSLFRPFITGATRSTSHVLLSANERSRFRPRMAVVAHSTSEARA
                     NAGAPSAPGPKKPVASGYVAWSLSVARHPYLTEHTLRGCPVLPACVTLDRIVSDVERG
                     TGRTARALRECQFLRAIRVPLGRSVELVTRWTPADGESTSLDVMIFGDVLTSRGEPLE
                     SQRCYAKCQLILEPTELPKRSPNWKDWPAGVPLEDPYLEHSSPIHLGPSFDCLEALTA
                     SEQGNRARYRPPLISLSDLPAIRLPTLLLDALLRVGVIGVDVVGEPRARRAKCVFVPT
                     YIEQIWLAEELGPSQAKQTCDLVGTNAKASSQGVECAWVAAHDDDGSPLITLTGYRGH
                     RVESESAGVEPTGIVDHCKSVA"
     gene            6534..7967
                     /locus_tag="VFQ61_31265"
     CDS             6534..7967
                     /locus_tag="VFQ61_31265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET9959027.1"
                     /translation="MKLDSQAGPPGPSLIDSLRYLTRLKTGDMRLYEKLFAEYGDVVR
                     LRCPGHEDVVFAFHPDDIHHVLCRNFRNYPKGKRYHELIPVLGMGLVNSDGELWRRQR
                     RLVQEQFNHENTLQFVPLIGRHIDALLARWDHLESPFVRDLNDDMLDVTFGVAGEAFF
                     GVGLAEHADTVRESFKFALSMALRRMNTLRPLPLSVPLPSHVRFSRAMAKIHDVIDAI
                     ITEYRTESRPANNVLQRLMNVVDPDTGNSMSHEQLRDEIKTILMVGHETSSVTATWAL
                     FLLARSPTARARLCREIDGVLAGRAPEVADFERMPFLNQVFNECLRLLPSVPFILRSP
                     IADDTLGGYRVRAGSTVVIAPWVTHRHPAFWKEPEEFRPERFSEGVKGLPHRLAFLPF
                     GAGQRICLGEFMGQVEGKMILTKLLQRYDLTLAPGFEPECRGFISLQPVNGMKMHFVR
                     REIAPRSVAPQAGELRDSKLANVAPAE"
     gene            8013..9119
                     /locus_tag="VFQ61_31270"
     CDS             8013..9119
                     /locus_tag="VFQ61_31270"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9959028.1"
                     /translation="MNSRLTIPSPEARIIDVVAKATRYPKELLGLDADLEDELGIDSV
                     KLQEILVALEQAFPGVPALPERERPRTLRDFVSWIQKTQPASADPSAPSSDPPLVAPE
                     FAATSAGAAPEASARHSLAGKIILVSGSGHGLGRATALELARRGATVVLNSFHHRELG
                     DEVARTIGAQGGQAHHIWASMARPDHVDRLFDELGDRFGGLDVFIHNASNGVFAKLGE
                     VTEEQWLKSFRTNVMGFHRGALRAAALMRKRGGGKILTLSSVYENATVDYFGVQGPVK
                     AALESLTRFLAKELLNDNIQVNCLSFGALEGKTMSLYPEAERIRRATEARSQGQRRMT
                     ELEAARAQLLFLSPDADSITGSTLRIDRGMMLSW"
     gene            complement(9330..10319)
                     /locus_tag="VFQ61_31275"
     CDS             complement(9330..10319)
                     /locus_tag="VFQ61_31275"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9959029.1"
                     /translation="MSAAPECQRAPDVNERIIVGNSAPLNRVLKRASQVASRDVTVLI
                     QGQSGTGKDMLASFIHGKSARASRPFVRFNSAAVPIPLAEAELFGHTRGAFTGATSAR
                     SGFVRQAHGGTLVFDEVGELPLELQAKLLRLLQDGEVQPLGASRVEHADVRILACTNR
                     DLRALVGQGKFREDLYYRLAVVELALPALAERRQDIPLLAREFALKYSRVFRIEEVSL
                     SAELLQRLSQREWPGNVRELENCIARMLALSTTGRLELEDLEDEPQKPCTCPPKGSLR
                     FQVSEFERQLIERTLKDCAHNQSEAARQLEITRVTLIDKMKRHGIHPRARAAN"
     gene            10553..11206
                     /locus_tag="VFQ61_31280"
     CDS             10553..11206
                     /locus_tag="VFQ61_31280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959030.1"
                     /translation="MRSTDESPYSSPELVRRQLGSGRADPTAFRAALRSIPEAERDAW
                     VDLVLELGDLPADSPELPRGCVPYLPCSVNTLLRMIELADVRCDDVFVDIGCGIGRAT
                     AVTHLLTGATAIGLEIQSALAGAARELAWRLNAPRLTVVEGDAAKLACELASGSVFFL
                     YCPFSGERLDRVIDDIGSAAKSREVRVCSVDLPIPARPWLTPVSVSGDLAVYRSASL"
     gene            complement(11388..11720)
                     /locus_tag="VFQ61_31285"
     CDS             complement(11388..11720)
                     /locus_tag="VFQ61_31285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959031.1"
                     /translation="MPTQDARPQLEEIASRLEDILTLTEGLEQPEFARSRITRQEVYR
                     QLQAAATDVCQLSSVVRETLAELDFGAWQKLRTELAENQGDPTGACWSALLQLVPDTL
                     SWLRVHGS"
     gene            12143..14323
                     /gene="tssI"
                     /locus_tag="VFQ61_31290"
     CDS             12143..14323
                     /gene="tssI"
                     /locus_tag="VFQ61_31290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HET9959032.1"
                     /translation="MFRIAEVSCALPEGTLLLLSMRGSETLGNPFRYVLDLLSLDDSV
                     DFGELLGRPMTLSLELADQSLREFNGLVTEFAIVGGLGRYVQYRAVLRPWVWLLSKRT
                     NCRIFQRKSVPTVVRELFKEHGFADFEERLTEKARYKELDYLVQYNESDLNFVMRILE
                     REGIYFYFRHEKGKHVLVLADSYSAHEPAKGAAEIPYFPPHQGQRREQDTLDGWLCSR
                     QIRSGAFVGRDFDFKKPYAAITPSAKHPHPHAQADFELFEYPADFLDAATGEQRVKMR
                     LEEHQADHEMVEAVGNVRAIGCGHLFKLKDFPREDQNKEYLIVGASYELEVNGYESKG
                     GVGSEMAYRVRVHAMDSKRPFRPARSTPRPVVEGPQTAIVRGDKSQEIWTDEFGRVKV
                     QFHWDRVGKNDEDSSCWIRVSQLWAGSGFGGIHLPRIGQEVIVDFLEGDPDRPIITGR
                     VYNQEQKPPYKLPDNATQSGIKSRSTLKGSPNNFNEIRFEDLKGKEELYIQAEKDQNT
                     LVKNNQSITVAVNQAVSVGANQSVSVGANQSISVGGHRTVTVVKSDTQTFVDKRTVTV
                     GKTDDLTITGIHTGNYNGTRILNVKGADNVTVKGGNKVTDVDGEYNMKAKTQVQAEQG
                     NNQLLLKGAQALLKNENCHVDLSGGVLTLKATSKIVLDVGGNMITIGSDGTIAISAKT
                     TASLSAEGGGAVKLDKAAAKVNGPMVGVAADVNVEITGALIKLN"
     gene            14371..15735
                     /locus_tag="VFQ61_31295"
     CDS             14371..15735
                     /locus_tag="VFQ61_31295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959033.1"
                     /translation="MRRALKDTKENLLEFVEQLDHCLLLVGMSDVEVPYVLKTLEQIR
                     AENPASLYVAFADEFQGEHDYGLAFAQRLEQLLTLENEERAAEKRELLAPFPPDFTAP
                     HTPAKQRIVLAFEHLRGWILDPTVDRVVIALVPMQAQPGPLYGKFVAQFAVHQGVRDP
                     WMAYGRVIGREDRTTHSTSDALNFVKADGMLAFDVDFSTEAMAEDLSKDAVDPSLPVA
                     DRMQAVLQLAAVDMSYKRYDEAVAKYVLLHGYYGSSGAPLMQSMCLLGIGDCMRMAGQ
                     VAMAKEKYQQGLAQVLSSRPAYPVPVPPLADGSPNPNPPLNPEGLPILLNLLLAAGES
                     SMSLQQWQDARDYFESASGVAARCMNPYAAADAMDRQGDAELMLGKPAEAIKAWADAE
                     KIAEAGGYTERRIGVLSKTQRLYEEAQHFPEARQAAERIERIRNQANGPKEPPPGEAQ
                     HRHA"
     gene            15773..20347
                     /locus_tag="VFQ61_31300"
     CDS             15773..20347
                     /locus_tag="VFQ61_31300"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HET9959034.1"
                     /translation="MTVGPILPIRYRSPRPVEPPAPVFATTSSATANAVLSTLDQAVA
                     PLRELPSSDDGAAGKVSKAVGGALGVINAPAMFIDAASSIGVSKLDQFISSKLGIPTL
                     FPALPVARLGITMHMGTPHTHVHPPSTTPPAPPIPLPSFGVAFLAGSASVLVNGIPAL
                     RAGDVGIGLTCGSMAPPFEIMTGAAGVYFGGARVARLGMDITFHCNPSPASAAAAAKV
                     AKPFMNVGKAAVAFGAMGAVAQGAGAIEAAEKGNSTAAAVQAAQMALDAAALALSMLR
                     GKDPAGPPGVGALIGSPPTVVAGGAPMPNLGAWAQGKLFSQIGKAVRGLKNKFKRKSP
                     AADANGTTSHVGEPVNLVTGENFNTYIDFKSYHGAFTWARYMTSAWARKPGRLGPGFR
                     HVLESRLEIRLHRVSFHGFAGEHIEFPRFGPEQSEVTLNGYRLTRLSDTRFRLRERRL
                     GTLEFQRPNDRTLTAALVAIQSPEARAELTYSDNGLLTEVREYSLGMLTPLAVYALSY
                     DSAGRLLAIQGCNLQPVGDARAASGPPAWQRLAVYTYDPEGHLISAEDPFGARESYVF
                     DKRHRLIRATDRGGYSFGWSYDIEDRCVSSAGQDGLWSVQIEYAPEARQTRVTFHHGG
                     VLVAHYDEDGVITKIIDANGATLDRLRAEDGRILEEVDSAGRRMVFLYDADGANIGRV
                     GPFGHRYLPELDEPVLGNPFERLVPDNARDRTLGTDRARELGLGQVLASLPAELSQLA
                     QRVFELGTSTPFEDELRTEHDSYGRRTLEIDALGRSRKWSYDAAGNEVMRSDREGRLY
                     QQRFTSFRLLAERVDPLGHSTSYSYDPHGRLTRITDPLGTTTEYAYDKRDRLVRVTRL
                     GSVREEYVRDKHGLVLEKRGKGSTPLLRITPHANCLPQTIELSDGKRLSFDYDARGQV
                     TVASSVEHEVTQAWDWRRRLVHASCDGREIEHAYDGPRQRETRIARKFSITHSRPAGP
                     NSAGTVDVRITGPNGFTWHLQRGQRGMVLLRHGDSADATQELQQYSLEGQLRARLVWH
                     PARLRQSSPSASWSARYEYSPEGDLLAIRDSLHGDRRFEVDAAHRLVAEVDSSGRRLS
                     YPLDEAGTLLSKPELPLVRIAPGNRLQHAGLESFEYDDRDRLCARWTRTRRTRYEYNS
                     LDQLVAAYRDEEPEPWTATYDGLGRRISCGIGDRRTIFWWDNDRLAAETLPDLTFRIY
                     IYADDTAFAPIGFVDYASVEAAPESGRAHSVFADQVGLPLLIQDAQGRVVWHTDDTDP
                     YGQIDVAADSTVAYNLRWPGHYFDPDTGLHYNRFRYYDPKLGRYLQSDPAGCAGGINL
                     YAYPSNPLIAVDVLGLTPNKTVSDADAPPAHLKTTEAAPAPEPTPPPKKGPSPLHGQE
                     ESHSCAIASARMVIEQKTGKDHGEGTLRNQSSADSNGYVPRRGTHLDNVERLLHNNGV
                     PEAQMKGGQSISDLQAATASGDPAIVAIVHPDGSGGHAMVCDGVIDNPDGSRSIVLRD
                     PAPQGSGTSTTMSESDFNKMGYSGWVITTN"
BASE COUNT         3701 a         6219 c         6688 g         3826 t
ORIGIN      
        1 cggtccaggg cccctaatct acgagccgta tccttacggc ggtggcggga acgctcgaat
       61 tcagctcatg ggcttgagtg acggggacat attctatgtc cccagtgagg gaagcccggc
      121 gcctctgcgt cgagttcgcg caatcgggca gaagcgcccc cctgttccag ctgcatgcaa
      181 tctttgagcg ctgtcgggcc tctttgcagc tgtcagagat tctgacagag ggggcccgaa
      241 cttcgagatg agagggcgct tcaacgagct cgtgtaagag cacggattgt ggcgccttct
      301 gggcttgcgc gaagtattgg ggcccctgcg gcacgcggct tgaagtagcg gcgggcagag
      361 tcgcgaacgt cgagctctct ggctcgatga gctcgctctc ccttcaggcg agtgctgaca
      421 tgcaggtttt tgcccaccgc tacctcatcc atttctcgga cacgatggca tacggcagcc
      481 atcacttcct tacgaacttc aagtttcagt gcgccgctcg agaagcgttg ctgttcctag
      541 cggagcccga cgggacggag ccctggcgat ccgaactcgg atccgtcgag ctgcttacgc
      601 gcgagggtta tacccggaat ctaagtccct tggggcttgg tgactgggcc gcagttctgc
      661 tgacggccga ggaggtgagt tacggctcat ttaggctgtg tttcagagtg gtctcgagcg
      721 cgggggagcc cgtcgcgtgt ggtcaccagc aaatcgtggt gctcgaccga gtgagtcgtc
      781 gagtctccgc gctgcccgag gtcatacagc gcaggctggc gcccatcgtc gagccagctg
      841 gcgcgacttt cgcggaacgc ttgcggcgag gcgggagtcg actggctcag ttgttccccc
      901 gagagttacg cgagctctcc gctcgcatcg cccgcggtga gttcatccac gagtcaagcc
      961 cttacttcga tattccaacc cggtccgcgc ccaacgaggc ggcgtcaccc gatcgcgtcg
     1021 cttggatttg tggagggcag ggggctctag atccagagtg ggcttggcgc gtcgcttgcg
     1081 cgcttcccaa ctccgcggag ctcttggacc aggcggacgc attgtgccga gccgcgtggg
     1141 gttcggacta tcgctcctgg ctgtcccgcg ggggtggtgg cggaggtgag ggccaacccc
     1201 ggtcggagca gatcttcaaa gaacaattga ccctctacct cggtgcggtg tttacggggc
     1261 gtatactcga agaaaatggc ttcgtcccag atttgatcct gggccatagt gtgggggaga
     1321 tcgccgcggc cgtcctcggc ggcgcactcg acgcgtccgc ggggctaacc gtcgtggcta
     1381 ctcgagcgtc ggtgcttctt cgccattgct ccggcgggag catgttggtg ctgcaggagc
     1441 gcgaatcatc tttagcaccc ctgttgagct cgttttctgg cctcacggtg gctgtccgca
     1501 atcatgatcg tcaactcgta gttgcagggc cacagggcac catcgagcag ctccgggggg
     1561 ccgcgcgcgc cgccggtatt gcctgcaaag ctttgccggg tttcgctttt cattcgtctt
     1621 atctgctccc ggccgtagct gagttctatg ctcaaaccga gccgttggcg ttcgcgtcgc
     1681 cgcgcgtgcc cgtgttttct cccatggaac agcggctcat cgactcgaga atcgatcgac
     1741 ccagtcactg ggcatcgcac ctaatccgta cgttggaatt cggtgcggct atgcgcacct
     1801 cggttggacc gcaaaactat cggttcgtgg agtgcgggcg tcggcttggc ttgggcaagg
     1861 tgctggaggc gctcggtgcg acctcggtct gttccgctca agacttgctc ggagagttcc
     1921 ctgcgggttc cgttgaaccc agtatggtcg acgtcccatc tcagctcgcc ccgttggccg
     1981 cgcccgagat cggaacgaac gcggagccaa tcgcgattgt cgcggtgggc tgccgcctgc
     2041 ccggagcgaa ggacttcgac gaatatcggc gcctgctcga gacgggcgaa tgcgccatcg
     2101 cagacctgcg cgagctctcc ccggagctcg aacgtgactt ctatacgaat actccgggac
     2161 cggacaagac ttacactctg ttgagtggtg tcgtcgacga gcccgacgcg gaggtgaatg
     2221 tcgagcgttc caagctcgcg aggctagtgc gggcagcgct cgcggagtgt cgcgtagggg
     2281 ccgtggcggg acgcagctgc gtcgtactcg gcgcaactgc cgatggttgt gccgagtatg
     2341 acgaggcgct ggtgctcgag cgactgtatg cggcggaggc ggccggttgt ccttccttac
     2401 gagccgcact ttcgcgcgaa atcgagcgga tcgggaccag ccgtacgctt caacccgcgt
     2461 ccaatctggc ccgagtggcc cgagagctct tgggagagac gacgcgaacc atattcgtgg
     2521 atgcggcgtg cgcgtcgtcc ttgtacgcga tcgctacggc ggcgcggctc ctcgccgaag
     2581 gcgagtgtga cgtggcctac gccggcggag cgttcgcccc gagcctctcc aacaattgct
     2641 tgtttgcaca gttcgagggg ctcgcgcaga ctggcagtta cccgctcgat cagcgagccg
     2701 aaggagtcgt gttcggggag ggggcggcct tcgtcgcgct gcggcggctc agcgacgccc
     2761 tcgcgagcgg cgagcgcgtg ttggcggtgc ttcgaggcgt aggcctctcc agcgacggtc
     2821 gtagcccgtc cgcgagtact ccgcgtgcgg acggccaaag gctcgcgatc gaacgagcct
     2881 atgtcgccag cggtatctca ccgcgatcgg tgcagtacgt cgaggcccac gcgaccggga
     2941 cagcggtggg cgacgccacc gagctcgagg cactatctcg cttctttcga gctgaaggcg
     3001 tgccggtggg cagcgtgcgg ctcggttcag tcaagagcca gatctcgcat acgggttggg
     3061 cggcgggtgt cgcgtcgcta atcaaggtcg tggcggctct ggccgagcgg aactaccccg
     3121 cgcaacatca ttgcaacgag cccagcacca aactcgaagg gtcgccgttt cgactcgagt
     3181 ctggggtcgg tgtaaaccac ggtggcgttc cctggaacgc aaatatcgag ggacagcctc
     3241 ggcgcgcagc cgtcaacggc tttgggttcg gcggcgccaa cgcgcatctc atcgtggaag
     3301 agttttctag ggaatttcat cagcagctca tcgccgcggc tcgtgtggct ccaaccaagc
     3361 aggttttgcg ggcaggcgtt ccactggagg ccaacctgcg gacgcccgac ctgaactcga
     3421 cgtggccggc cggagctttg cgcctcctcc ccgacgtggt cgagaccctc gatgccacgc
     3481 agtggaccgc ggcggtgctt gcctcgcaag ctttggagcc tctgctgggg accgcagggg
     3541 gggaccgtaa ccgtatcgga gtggtggtcg ggatgcgcgg gaaaacctcg acgattcttc
     3601 gcgccaacga ccgcattttt cgcgatcgcg tgctgaggat ttcgaccgcc gactcggggc
     3661 cgctcgagcg ggcggcgatc acagagtcgg tgatgcgctg ggcaggctcg gcgccaagct
     3721 cgacgcctta cacattgacg ggaggcatgc ccaatttggt ctcgggccga gtatgccagt
     3781 tgttcgactt gaaaggtccg aattgcgtgt tcgacgccgc gacggacaca ctcgaaagcg
     3841 ccatgctgca ggcgcgagcc tggatcgcgg aaggaacctg tgactgggtc ctggcgttcg
     3901 ggatcgacgc ggtggaatcg gaccactccc aggcacgggt gcagctgctg ggtgaagcgg
     3961 cacgagtcgt tgctctcgcc ctgcctgctt cggagctctc ggcgcccaga gctgcgaact
     4021 tcgagcagcg caccgacctt gcggcacctc ccgcagatat tcagttgttg cgcccggttc
     4081 tggtcgacct ggattggcgc tcccctggac caacgagcga ttctgaagca gtggtgtcgt
     4141 tcagtccggc ctcgttgggg agcgaggcaa gggtgcgctt cgaccgcgcg cggtatcgct
     4201 cggatgaaac catcatcgac gcgcaactcc cgactaacga accaactcgg gtgctcctgg
     4261 ttctcgatct ggccggagat cctttatccc tggtccagcc cggagccgtc cgtgaacgtg
     4321 acgtcctcgc gccactctgg gcctttgcgc gtgaacacta tgcgagcctg gagcagaacc
     4381 gaatggcttg gggcgtcgtt tgtttgaatg cgtgggtcga ttcgaacaca ccgcgcccgg
     4441 aaaccggcct cgtcggcggc ttcatgaagt cgctggcgcg cgagttgccg caggctcgac
     4501 tgatcgcggt gcatacggac gccgcgctcg atgcaggact gagctgcgcc tccgaggggc
     4561 tcctcggcgc taccgatgaa cgcgaagtct tcgtcctgga cggccgcaag cgcgtccacg
     4621 ccttgtcacc ggtagagttc gagactggcg gccccccgca gcttcgggcg gacagcgtcg
     4681 tgctggccac aggtggcgcc cgcggcatta cggcagagct cgtggcggcc atccttcagc
     4741 gctttggctg ccgagtggcg ctactggggc gaagcgatcc cgagttggtt ccgccgcacc
     4801 tccgtggtgc gtccgcacaa accctggaag ccgcgcagtc cgagttcgtt cgcggagcgg
     4861 tagcttcggg ctcgagcccg cgccaagcga aagcggcgtt tgccgagctc gccgcggcgc
     4921 acgaggtgct ccaaaacctg agtcgcttcc agaagctccc gggagaggtg agttacttgc
     4981 gttgtgacct gacagagccc gaggatgtgg cccggtcgat aaacgctgtc gcacagcggt
     5041 ggggtacggt gagtttcgtg ctgcatggcg ccggcgtaca gacctcgagc aagctcaatc
     5101 ggaagacacc ggaagccttc tgggccgtgc tcgacgtcaa gatcgcaggg ctgcgcaatc
     5161 tcctgagcgc gctgcagact cagtttccag accattgccc gcagctacac gtggccacct
     5221 cggccttcag ctacttcggt aacgacggtc agcaggacta cggcgccgcc aacgaggcgc
     5281 tgaatcggct cgcggattgc gcgagcgcac ttggacagcg ctggtccact ctgggctggc
     5341 tggcttggga cggtgttggc atgacccgcg ggagcgagta ccgagcgctg gcccgggcgc
     5401 gagaaatgcg ggcgctgagc gccgaggagg ggcagtctct attcaggcct ttcataacgg
     5461 gcgccacgcg gtcgacgagc cacgtgctgc tcagcgcgaa cgagcgctct cggttccgtc
     5521 cacgcatggc cgtcgttgcg cactccacaa gcgaagctcg cgcaaacgcg ggcgccccat
     5581 ccgcgcctgg gccgaagaag cccgtcgctt caggctatgt ggcatggtcg ctctccgtgg
     5641 ctcgccaccc gtaccttacc gagcacacac ttcgaggttg cccggtactg cccgcgtgcg
     5701 tgaccctgga ccgcatcgtg agcgacgtgg agcgcggcac ggggcgcacc gcgcgagctt
     5761 tacgagaatg ccaattcctg cgcgcgatcc gagttccgct gggtcgaagc gtagaactcg
     5821 tcacacgttg gacacccgcc gatggtgagt cgaccagcct cgacgtcatg atctttggcg
     5881 acgtgctcac gtctcgcgga gagccgctcg agtcgcaacg gtgctacgcg aagtgccagc
     5941 tgatactcga gcccacggag ctgccgaagc ggagcccgaa ctggaaggac tggcccgctg
     6001 gagttcctct cgaagatccc tatctcgagc actcctctcc gatccatctc ggtccgagct
     6061 ttgattgcct cgaagccttg acggcaagtg agcaggggaa tcgcgcgcgc taccggccac
     6121 ccctgatctc actcagtgac ttgcccgcta ttcgtcttcc cacgttgtta ctcgatgctt
     6181 tgctgcgggt cggggtcatc ggggtcgacg tagtgggcga gccgcgcgcc cgacgcgcca
     6241 agtgcgtctt cgttccgact tatatcgagc aaatttggct ggccgaggag ctaggcccga
     6301 gccaagcgaa gcaaacctgt gacttggtgg ggacgaacgc caaggcctcg tcacagggcg
     6361 tggagtgcgc ctgggttgcg gcacacgatg atgacgggtc acccctgatc acgttgactg
     6421 ggtatcgcgg tcatcgggtg gagagcgaga gtgccggggt cgagcccacc ggcattgtcg
     6481 atcattgcaa gtcagtggcc tgatctcgaa tctaagacgg taaaggtgtt ttcatgaaac
     6541 tcgactccca agcggggccg ccggggccct ctctaatcga cagtctgcgc tacctcacgc
     6601 gtctcaagac cggcgatatg cggctctacg agaaactgtt cgccgagtat ggggacgtgg
     6661 ttcgtctgcg ttgcccaggg cacgaggatg tggtgttcgc cttccacccc gatgacattc
     6721 accacgtatt gtgccggaac ttccgcaatt accccaaggg aaagcgctac catgagctca
     6781 ttcccgtgct cgggatgggc ctcgtcaaca gtgacgggga gctttggcgt cggcagcggc
     6841 gcctggtgca agagcagttc aaccacgaga atacgttgca gttcgtgcct ctgatcggtc
     6901 gtcacatcga cgccttgttg gcgcgttggg accatctcga atcaccgttc gttcgagatc
     6961 tcaatgacga catgttggac gtcacctttg gagtcgccgg cgaagcgttc tttggagtgg
     7021 ggctggccga acacgcggat accgtccgcg agtccttcaa gttcgcgctc tcgatggcgc
     7081 tgcgtcgcat gaacacgctg cgaccgctgc cgctgtccgt acccctgccg agccacgtcc
     7141 gcttctcccg tgccatggcc aagatccacg acgtgatcga cgcaatcatc accgaatacc
     7201 gtacggagtc ccgacctgcc aacaacgtgc ttcagcgatt gatgaacgtg gtggaccccg
     7261 ataccggaaa ttcgatgtcc cacgagcagc tccgggacga gatcaaaacc atcctcatgg
     7321 ttggccacga gacctcgagt gtgacggcca cctgggcgct ttttctgctg gcccgctccc
     7381 ccacggcccg ggcgcggcta tgccgagaga tcgatggcgt gcttgcaggt cgagccccgg
     7441 aggttgccga cttcgaacgg atgccgttcc tgaaccaagt cttcaatgaa tgtttgcgcc
     7501 tcctgccgtc cgttcctttc attctgcgaa gtccaatcgc ggatgacacg ttgggtggct
     7561 atcgcgtgcg cgccggctct accgtggtca tcgcgccctg ggtcacgcat cgccatcccg
     7621 cgttttggaa ggaaccagaa gagtttcggc cggagcgatt ttctgaggga gtcaaaggct
     7681 taccgcatcg tctcgcattt ctaccgttcg gggccgggca gcggatctgc ttgggcgaat
     7741 tcatgggtca agtagaaggc aagatgatcc tcacgaagct gctccagcgc tacgacctca
     7801 cgctggcccc cggcttcgag cccgagtgtc gcggcttcat ctcgctgcaa cccgtgaatg
     7861 gaatgaagat gcactttgtg cgtcgtgaga tagcgccgcg ctccgttgcg ccccaagctg
     7921 gcgagctgcg tgactcgaag ctcgcgaacg tcgctcccgc cgagtgaacg gctttgcctc
     7981 tgtatccgct agtcgaaagg aaacctgcgc tcatgaatag tcgacttaca atcccgagcc
     8041 ccgaggcgcg aatcatcgac gtcgtcgcca aagccacgcg ctaccctaaa gagttacttg
     8101 gtttggacgc cgatttggag gacgagctgg gcatcgactc ggtcaagctc caggagatct
     8161 tggtcgcgct ggagcaagcc tttccggggg tgcccgcgct gccggagcgg gaacgaccgc
     8221 gaacgctcag ggacttcgtg agttggatcc agaagacgca gccagcgtca gcagaccctt
     8281 ccgctccctc gtctgaccct ccactcgtgg cacccgagtt cgcggccacg agtgcgggcg
     8341 cagcgccgga agcttcggct cgtcactctc tggcgggcaa gatcatcttg gtgagcggct
     8401 cgggccacgg cctcggtcgg gccaccgcgc tcgagctcgc acgccgcggc gcgacggtgg
     8461 ttctgaacag tttccaccac cgcgaactgg gcgacgaggt ggctcgcacg atcggcgcgc
     8521 aaggtggcca ggcccaccat atctgggctt cgatggcgcg accggaccac gtagatcgtt
     8581 tgttcgacga gctcggagac cgcttcggcg gactcgacgt gttcattcac aatgcctcga
     8641 atggcgtatt cgccaagctc ggagaagtga ccgaggagca atggctcaag tcctttcgta
     8701 ccaacgtcat gggctttcac cggggcgccc tgcgtgcagc ggcgttgatg cgcaagaggg
     8761 gcggaggcaa gattctgacc ctgtcttccg tctacgagaa cgccaccgtg gactacttcg
     8821 gcgtgcaagg gccagtcaaa gccgctctcg agtctttgac gagatttctc gcgaaggagt
     8881 tattgaacga caacatccaa gtcaattgtc tctcgttcgg agcgctggag ggcaagacga
     8941 tgagcctcta cccggaagcc gagcgcattc gcagggcgac ggaggcccgt tcacagggcc
     9001 agcggcgcat gaccgagctc gaggctgccc gcgcccagct gctctttctg agcccagacg
     9061 cagattcaat tactggatcg accctgcgga ttgatcgcgg catgatgctg tcgtggtgaa
     9121 tgggtctacg aaggggggaa aggaggggcc tcggggcgtt cgcgcttgga cgcacggtgc
     9181 tctcgatggc ccctcccatg gcgcgcggag cgcgacatcg gggaggggcg gcggcacgcg
     9241 caggcggcga tgaggacctg tatcttcgcc aagaaggatc tcgcggccgc cgagcatggc
     9301 gcggggtggg catcatccga atgtgagtgc tagtttgcgg cgcgggcccg cgggtgaatc
     9361 ccatgccgct tcatcttgtc gatcagcgtg acccgggtga tctcgagctg acgggccgcc
     9421 tcactctggt tgtgggcgca atccttcaaa gtccgttcga tcagttgccg ctcgaactcc
     9481 gagacttgga agcgcagtga gcccttgggc gggcaggtgc acggtttctg gggttcgtcc
     9541 tcgagatcct cgagctcgag ccgtccggtc gtgctgagcg cgagcatgcg agcaatgcag
     9601 ttttcgagct ctcggacgtt acccggccat tcacgctggc tcagccgctg caggagctcg
     9661 gccgataaac tcacctcttc gatgcggaac acccgcgaat acttgagcgc gaactcgcga
     9721 gcgagcaggg ggatatcttg tcttctctcc gccaacgcag gcagcgctag ctcgaccacg
     9781 gccaaacgat agtagagatc ttcgcgaaac ttgccttggc cgacgagcgc gcggaggtca
     9841 cgattggtgc aagctagaat cctcacgtcc gcgtgttcga cccgcgaagc gccgaggggc
     9901 tgcacctccc cgtcttgcaa cagccgcaag agcttggctt gcagttcgag ggggagctca
     9961 cccacctcgt caaaaacgag ggttcccccg tgggcctgtc gaacgaagcc gctccgagcg
    10021 ctagtggcgc cagtgaacgc gccgcgcgtg tgcccaaaca gctccgcctc cgccagcgga
    10081 attgggactg cggcactgtt gaagcgcacg aaggggcgag atgctcgggc acttttaccg
    10141 tgaatgaagc tagccagcat gtccttcccg gtgccgctct gaccctggat caacaccgtc
    10201 acgtcccggc tcgcgacctg actcgctcgc ttcaacactc ggttcagtgg cgcgctgttt
    10261 cccacgatga tcctctcgtt cacgtccggg gctctttggc attcgggtgc agcactcatc
    10321 ccgctaacct aggggactca gtcgaccgag ggctgacata gagtttccgt gcacgcgctg
    10381 aagtcgtggt tcatctcgac ccggatggca ggtgagcccg agacgcgcct cgtgcagtcg
    10441 ggctctgctc gggcgagctc ttgcggtagt cttcggaccc atttccgcga tcgaatgcgc
    10501 ccggcgcgtg gagtcgtgac ccgtgggccc cgggattata gactggcgcc gtatgcgttc
    10561 gactgatgag agcccctatt cgagcccgga gttggtgcgt cgccagctcg gttcgggacg
    10621 agcggatcca acggcgttcc gcgcggcctt gaggagcatt cccgaggcgg agcgtgacgc
    10681 ctgggtcgat ctcgttttgg agttggggga cctccccgca gactcgcccg aactgccacg
    10741 gggctgtgtc ccgtatctac cctgctcggt gaatactctg ctgcgcatga tcgagctcgc
    10801 ggacgttcgt tgcgacgacg tgttcgtcga tattggctgt ggcatcggca gggccacggc
    10861 cgtgacgcac ctcctgacgg gtgcgaccgc gatcggactc gaaatccaat cggccctcgc
    10921 cggggccgcc cgggagctag cctggcgact gaatgcgccg cggctcaccg tggtcgaggg
    10981 agacgccgcg aaactcgcct gcgaattggc aagcggatcg gtcttctttc tgtattgccc
    11041 cttcagcggt gagcgactcg atcgggtcat cgacgacatc gggtccgcgg caaagagccg
    11101 tgaggtgagg gtgtgcagtg tcgacttgcc catcccagcg cggccatggc tcacgcccgt
    11161 atccgtctcc ggcgatctgg ctgtgtatcg tagtgcgtcg ctctgactac cagcgtgtct
    11221 catggtgatg caaatcggtc gttggatcgt cgccacggag ctacgggcgc gaggcgcgga
    11281 ttggggattc gcgtgcgcag ccgctcgtgc acgatgatac aaaccacacc gcgcctgcga
    11341 ctcgcagtcg acgctcgggt agggacccgt ggcttcgcgg atttcagcta ggaaccatga
    11401 acgcgcaacc agctgagcgt gtcgggaacg agctgcagta gagcggacca gcaggcgccc
    11461 gtcgggtcac cctgattctc ggcgagctcg gttcgcagct tctgccaggc cccgaagtcg
    11521 agctcggcga gcgtctctcg caccactgag ctcaactggc aaacgtcggt ggctgcggcc
    11581 tggagttgac ggtacacctc ctggcgggtg atgcgggagc gggcgaattc gggctgctcc
    11641 agcccctcgg tcagggtgag gatgtcttcg agacgagagg caatctcttc gagctgggga
    11701 cgagcatctt gcgtcggcat cgtgctacgt gccttgcaag ctctcggcct cggaaggcct
    11761 ggcatggcat tgcacggccc agccacattt ggctcgcaat cgcggttccc cggatcgccg
    11821 ccaggagcgc gcgtttggta cgagacgcgc cctgttgtcc gcttctcgac aagaggccat
    11881 ccctcgggtt cgcgggtggc gccctctgca aatctcacat gatcaacgct acacgtgggg
    11941 aagacccttg ctccgggggg cgtatgcgga aggtgtggag cctctagacc agacccagcg
    12001 cgcggatgat caatcggagc cgtggcggca caaaacgcgg gtttgaaggc gatgaacggg
    12061 gcactgacag ttgactggca cgggatctcg tggtggactc ccgcaagtca gggtatcggt
    12121 cggggtgttt gggtaaaaaa acatgtttcg aatcgctgaa gtttcgtgtg ctctgccgga
    12181 aggaacgctg ctgctcctgt cgatgcgcgg aagtgagacg ctcggcaatc cgtttcgcta
    12241 cgtgttggat ctgttgagcc tcgacgatag cgttgatttc ggcgaactgc tcggtcgacc
    12301 gatgaccctc tccttggagc tcgcggacca gagtctgcga gagttcaacg gtttggtgac
    12361 cgagtttgcg atcgtcggag ggctcggccg ctatgtccag tatcgggcgg tgctgcggcc
    12421 ctgggtttgg ttgctcagca agcgcaccaa ctgccgcatc ttccagcgca aaagcgtacc
    12481 aaccgtggtt cgcgagctgt tcaaagaaca tggcttcgcg gacttcgagg agcgattgac
    12541 cgagaaggcg cgctacaaag agctcgacta cttggtgcaa tacaacgaat ccgatctgaa
    12601 cttcgtgatg cggattctgg agcgggaagg aatctacttc tactttcgtc acgagaaggg
    12661 caagcacgtc ttggtcttgg cggactcgta cagcgctcac gagccggcca agggcgccgc
    12721 cgagattccg tatttcccac cgcaccaggg ccagcggaga gagcaagata cgctggatgg
    12781 ctggttgtgc tcgcgtcaga tccgatccgg agccttcgta gggcgggact tcgacttcaa
    12841 gaagccctac gcggccatca caccctccgc aaagcatcct cacccgcacg cgcaggctga
    12901 cttcgagctg ttcgagtatc cggcggactt cctggatgcc gccaccggag aacagcgggt
    12961 gaagatgagg ctcgaggagc accaagccga ccacgagatg gtagaggccg tcggcaacgt
    13021 gcgggcgatc gggtgcgggc acctgttcaa actcaaggac tttccccgcg aggaccagaa
    13081 caaggagtac ctcatcgtcg gcgcgtccta cgaactcgag gtgaatggct acgagtccaa
    13141 ggggggcgtt gggagcgaga tggcgtatcg ggtccgcgta cacgccatgg atagcaagcg
    13201 cccatttcgc cctgcgcgca gcacgccccg gcccgtcgtc gaggggccgc aaaccgcgat
    13261 tgttcgcggc gacaagagcc aggagatatg gactgacgag tttgggcggg tcaaggttca
    13321 gttccattgg gaccgcgtgg gcaagaacga cgaggacagc tcgtgctgga ttcgcgtctc
    13381 ccagctctgg gccggtagtg gcttcggcgg tatccacctg ccccgaatcg gccaggaagt
    13441 gatcgtggac ttcctggagg gggaccccga tcggccgatc atcacgggcc gcgtctacaa
    13501 tcaagagcag aagcccccct acaaactgcc ggacaacgcg acccaaagcg ggatcaagag
    13561 ccgcagtaca ctcaagggct cgcccaacaa cttcaacgag attcgcttcg aagacctgaa
    13621 gggcaaagag gagctctaca ttcaagcgga gaaggaccag aatactctgg tcaagaacaa
    13681 tcagagtatt acggtagccg tcaatcaggc ggtctcggtg ggtgcgaatc agagcgtctc
    13741 ggtaggcgcg aatcaaagca tctcggtggg tgggcaccgc accgtgacgg tggtcaagtc
    13801 agatacccag actttcgtcg acaagcgtac ggtgacggtg ggcaagaccg atgatctcac
    13861 gattacgggc attcacaccg ggaactacaa cggcacgcgc attctgaacg tcaaaggcgc
    13921 cgataacgtc accgtcaagg ggggcaacaa agtcaccgac gtggacggcg aatacaacat
    13981 gaaggccaag acgcaggtgc aggccgaaca aggcaacaat cagctgctgc tcaagggcgc
    14041 acaggcgttg ctcaagaacg agaactgcca tgtggatctg agcggcggcg tgctcacgct
    14101 caaggcgacc agtaagatcg ttctggacgt gggcggcaac atgatcacga tcggatcgga
    14161 tggcacgatc gccatctccg ccaagacgac ggcgagcttg agcgccgaag gcggaggcgc
    14221 ggtcaagctg gataaggcgg cggccaaggt gaacgggccg atggtgggcg tggccgccga
    14281 cgtcaacgtc gagattaccg gcgcgctgat caaactaaat tgagaaatcg ggaacgtccc
    14341 tggcagtaat actcggcccc cggaggcatc gtgcgtcgcg cgctgaaaga taccaaggag
    14401 aacctgctcg agttcgtgga gcagctcgat cattgcttgt tgctcgtcgg catgagcgac
    14461 gtcgaggtac cgtacgtgct gaagacgctg gagcaaatcc gggccgagaa cccagcctcg
    14521 ctgtacgtcg cgttcgcgga cgagtttcag ggcgagcacg actacggctt ggccttcgcg
    14581 cagcgcctgg agcagctgct gacgctcgag aacgaggagc gcgccgcaga gaagcgcgag
    14641 ttgctggcgc ccttcccgcc cgatttcacg gcaccgcaca cgcccgcgaa gcaacgcatc
    14701 gtgctggcct tcgagcactt gcgcggctgg atcctggatc cgaccgtcga tcgcgtggtg
    14761 atcgcgctcg tgccgatgca agcgcagccg gggccactgt atggcaagtt cgtggcgcag
    14821 ttcgcggttc atcaaggcgt gcgcgatccc tggatggcct acggtcgcgt gattggtcgc
    14881 gaggatcgaa ccactcactc tacttcggac gcgctgaact tcgtgaaggc ggacggcatg
    14941 ctggctttcg atgtggattt cagcaccgag gcgatggcgg aggacctctc gaaggacgcg
    15001 gtcgatccga gcctgcccgt agccgacaga atgcaggccg ttttgcagct cgcggcggta
    15061 gacatgtcgt acaagcgcta cgacgaagcc gtcgccaagt acgttctgct gcacggctac
    15121 tacgggagca gtggcgcacc gttgatgcag tccatgtgcc tgctcggtat tggagattgc
    15181 atgcgcatgg ccgggcaggt cgcgatggcc aaagagaagt accaacaagg gctcgcgcag
    15241 gtgttgagct cgcgaccggc ctatccagtg ccggtcccgc ccctggccga tggtagcccc
    15301 aaccctaatc cgccgctcaa ccccgaggga ttacccatct tgctcaatct gctgctggcg
    15361 gcgggcgaga gcagcatgtc tctgcaacag tggcaggatg cgcgcgacta cttcgagagc
    15421 gcgagcggcg tcgcggcgcg ttgcatgaac ccgtacgcgg cggcggatgc catggatcgc
    15481 caaggtgacg ccgagttgat gctcggtaag ccggcggagg cgatcaaagc gtgggcagac
    15541 gcggagaaga tcgcggaggc cggcggctat acggagcgcc ggattggcgt gttgagcaag
    15601 acgcaacgcc tttacgagga agcgcagcat ttcccggagg cacgccaagc cgccgaacga
    15661 atcgagcgca ttcgcaacca ggcgaatggg ccgaaagaac cgccccctgg tgaagcgcag
    15721 caccgacacg cttgagcggt gacgaaccga tcgagttgga aggatagatg acgtgacggt
    15781 tggtccgatt ctgcccatac gctaccgcag cccgcgcccg gtcgagccac cggcgccggt
    15841 attcgcaacg accagcagtg cgaccgcgaa tgcggtcctg agtacgttgg accaagcggt
    15901 agctccgctg cgcgagctgc cgagtagtga cgacggggcc gccggtaagg tttcgaaagc
    15961 ggtgggtggc gctctgggcg tgattaacgc gccagcgatg ttcatcgacg ccgcgtcttc
    16021 gatcggcgtg tcgaagctag accagttcat ctcgagcaaa ctcgggattc cgacgttgtt
    16081 cccggctttg cccgtggcgc gcttgggcat cacgatgcac atgggcactc cccacaccca
    16141 tgtgcatccg ccgagcacga ccccgcccgc gccgccgatt ccactgccga gcttcggagt
    16201 tgcgttcctc gcgggctcgg cgtcggtgct ggtgaatggg atcccggcgc tacgcgcggg
    16261 cgatgttgga attggcctga cttgcggctc gatggcgccg ccgttcgaga tcatgactgg
    16321 ggccgcgggt gtttacttcg gcggcgcacg cgtggctcgc ttggggatgg atattacctt
    16381 ccactgcaac ccgagcccgg cgtcggcggc ggccgcggcc aaggtcgcca agcctttcat
    16441 gaacgtcggg aaggcggcgg tggcgttcgg ggcgatgggc gcggtggcgc aaggcgcggg
    16501 cgcgatcgaa gcggcggaga aggggaatag cacggcggcg gcggtgcaag ccgcgcagat
    16561 ggcgttggac gcggcggcgc tcgcgttgtc catgttgcgg ggcaaggatc ccgcgggtcc
    16621 accgggcgtc ggcgccctga ttggttcgcc tccaaccgtg gtggccgggg gcgcaccgat
    16681 gcccaatcta ggagcttggg cgcaaggcaa gttgttcagt caaatcggga aggcggtgcg
    16741 cgggctcaag aacaagttca agcgtaaatc gcccgccgcc gacgcgaacg ggaccacgtc
    16801 gcacgtgggg gagccggtca acctcgtcac gggcgagaac ttcaacacct atatcgactt
    16861 caagtcgtac cacggcgcct tcacctgggc gcgctacatg accagcgcgt gggcgcggaa
    16921 acccggccgt ttggggccgg ggttcaggca cgtcttggag tcgcggctgg agattcgatt
    16981 acatcgagtc tcgtttcacg gctttgccgg agaacacatc gagtttcccc gttttggccc
    17041 cgagcagtcc gaggttactc tcaatggcta ccgtctgacc cgactgtccg atacccgctt
    17101 tcgattgcgc gagcggcgct tgggcacgct cgagtttcag cgcccgaacg accgcacgct
    17161 gacggctgcc ttggtggcga tccagagccc cgaggctcgc gccgagttga cctattccga
    17221 caatggcctg ttgaccgagg tccgcgagta ttcgttgggt atgctcacgc cgctagcggt
    17281 gtacgcactc agctatgatt ccgcggggcg gttgctggcg attcaaggct gcaacttaca
    17341 gcccgtgggc gatgcacgag ccgcgtcggg gccgccggct tggcaacgtt tggccgtata
    17401 tacctacgat cccgaaggcc acctgatctc tgccgaagat ccgttcggag cgcgcgagag
    17461 ctatgtcttc gataagcgcc atcgcctgat tcgtgcgacg gatcgcggag gttattcctt
    17521 cggctggagc tacgatatcg aagatcggtg cgtatcgagc gccggtcaag acgggctctg
    17581 gtcggtgcag atcgagtacg cgccggaagc ccggcaaacc cgggtgactt ttcaccacgg
    17641 tggcgtcttg gtggcgcact acgatgaaga tggggtcatc accaagatca tcgacgcaaa
    17701 cggcgccacg ctggaccgcc tgcgcgcgga ggacgggcgg atcctcgaag aggtcgattc
    17761 ggccgggcgc cgcatggtgt tcctgtacga cgccgacgga gccaacatcg ggcgggtggg
    17821 tccctttggt catcgttact tgcccgagct cgacgagccc gttttgggca atcccttcga
    17881 gcgtctggtg cccgacaacg ctcgggatcg gacgttggga actgaccgcg ctcgcgaact
    17941 cggcctcggg caagtattgg caagcttgcc ggcggagctc agccagcttg cccagcgcgt
    18001 gttcgaactg gggacctcca ctccgttcga agacgagctg cgcaccgagc acgattcgta
    18061 cggccgtaga accctcgaga ttgatgcgct cggtcgttct cgcaagtgga gctacgacgc
    18121 ggccgggaac gaagtcatgc gctcggatcg agagggacgt ttgtaccagc agcggttcac
    18181 gagctttcgt ctgctggcgg agcgggtgga tccgctcggg cattccacgt cgtactcgta
    18241 cgatccccac gggcggctga cccggattac agacccgctc ggaaccacga ccgagtacgc
    18301 gtacgacaaa agagaccggc tggtccgcgt cactcgactg gggagcgtgc gtgaggaata
    18361 cgtgcgcgac aaacacggac tcgtgctcga gaagcgcggc aagggttcca cgcctctttt
    18421 gcggatcact ccgcatgcca attgtctgcc ccaaaccatc gagctttctg acggcaagcg
    18481 tctgtcgttc gactacgacg cgcgtggcca agttacggtg gccagcagcg tcgagcacga
    18541 agttactcaa gcctgggact ggaggcggcg gctcgtccac gcgagctgtg acggccgtga
    18601 gatcgagcac gcctacgacg gccctcgcca aagagagacc cgaatcgcgc gcaagttctc
    18661 gatcacgcat agccgcccgg cggggccgaa ctccgcgggt acggtagacg tccgtatcac
    18721 cggtccgaac ggcttcactt ggcacttgca gcgtggtcag cggggaatgg tcctgctccg
    18781 acacggtgat tctgccgacg caactcaaga gctgcagcag tactccctcg aggggcagct
    18841 ccgcgcgcgc ttggtatggc acccggcccg gctgcgccaa tcgagccctt ccgcgagttg
    18901 gtcggctcgc tacgaatata gccccgaagg agatctatta gcgatccgag acagcctgca
    18961 cggagaccgt agattcgaag tggatgcggc ccaccgcttg gtggcggagg tcgactccag
    19021 tgggcgtcgt ttgagctatc cgctcgacga agccgggacc ctgctcagta agccagaact
    19081 gccgctggtc aggattgccc ctgggaatcg gctccagcac gcgggcttgg agagcttcga
    19141 atacgatgac cgagatcgac tgtgtgcgcg ttggacgcga acccggcgga ctcgttacga
    19201 gtacaacagc ttggaccagc tcgttgcggc gtatcgagac gaggagcccg agccgtggac
    19261 cgcgacgtac gacgggctcg ggcggcgcat cagctgcggt ataggtgacc gacggacgat
    19321 cttctggtgg gacaacgacc ggctcgccgc ggagactctt ccggatctta cattccgaat
    19381 ctacatctac gcggatgaca cggcgttcgc gccgattggc ttcgtcgact acgcatcggt
    19441 cgaggccgca cccgagagcg gccgtgctca cagcgtcttt gccgaccagg tggggttacc
    19501 gcttctcatt caggatgccc aaggccgcgt ggtttggcac accgacgata ccgatccata
    19561 cggacagatc gacgtcgcgg cggactcgac ggtggcctac aacttgcggt ggcccgggca
    19621 ctacttcgac ccggacaccg ggctgcacta caaccgcttt cgctactacg accccaagct
    19681 cggtcggtat ttacagagcg atcccgcggg ctgcgcgggc ggcattaacc tctatgccta
    19741 tccatcgaat ccgctgatcg ccgtcgacgt gttggggctc accccgaaca agacggtatc
    19801 ggacgcagac gcaccaccag cgcatctgaa gacgaccgag gccgcgcccg cgcctgagcc
    19861 aactccgcca cccaagaagg gccctagccc tttgcacggg caagaggagt cgcattcatg
    19921 tgccatcgct tcggcccgta tggtgattga gcagaagacg ggcaaggatc acggtgaagg
    19981 tacgctacgc aatcagtctt ccgcagattc taacgggtat gtaccgagaa ggggaaccca
    20041 cctggacaat gtggagaggc tgctccacaa caacggcgtt cccgaggcgc aaatgaaggg
    20101 gggccagtcc atatcggact tgcaggctgc gaccgcatct ggagatccgg ccatcgtcgc
    20161 gatcgtccac cctgatggga gcggcggtca cgccatggtt tgtgacggtg tcatcgacaa
    20221 ccccgacgga tcccggtcta tagtgttgcg cgaccctgcg ccacagggta gcgggacgtc
    20281 aactaccatg tcagaaagcg atttcaacaa gatgggttat agtggctggg tgattactac
    20341 gaattgaggg ctgaatggaa ccgagcgaca ttgttcgtgg cacggcagcg aagtactttg
    20401 gcactgcgct ttcgagtgtc ttcgtggaca agga
//
LOCUS       DASTKU010000891         8317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1066961, whole genome shotgun sequence.
ACCESSION   DASTKU010000891 DASTKU010000000
VERSION     DASTKU010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8317
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066961"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFQ61_31305"
     CDS             <1..583
                     /locus_tag="VFQ61_31305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959035.1"
                     /translation="PAECRTEFGEAGKDRCFGRAGLRELIAHPGHFLALIPRKLSATF
                     DWSGAPGHYLTASNSSALSADSALLLGIVEAVVLRLSLLACLWRVGVADGPRRRLRRS
                     VCLLSAPWLFLRPAWISYLGLCMAGLSFGRDLDRRPNVALGVAAVASTALVHAVFFGA
                     GRYGLVCVLALLPLIAERRSHTLEPTEVTLHAR"
     gene            832..1095
                     /locus_tag="VFQ61_31310"
     CDS             832..1095
                     /locus_tag="VFQ61_31310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959036.1"
                     /translation="MPLIEVEEAARRLARAIASDLSLYNEEKIIRGIEGDCLFEELAE
                     EIEEGRTLYKSRVSPDLYHRNYYDRALVDILVKSKGHIKSKIW"
     gene            1092..2051
                     /locus_tag="VFQ61_31315"
     CDS             1092..2051
                     /locus_tag="VFQ61_31315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET9959037.1"
                     /translation="MTRLRFVLGPGERERLDKVLVRLCEGASRATVQRWVEEGRVRVN
                     GKACRAKDQVGPGAVLEVEPAPPPLSTAEPDAGVRFELLYEDDELLVVNKPAGLVVHP
                     ARGHATGTLVNGLLSMQSFRSVPVDPRDPAGVLRPGVVHRIDKGTSGVLVIAKNERVR
                     EGLKEQLAKHTVERVYCALTIGVPQAGRIATLYGRHPQNRLKFSSRVAQGKTAVTHVS
                     VLETLAGGRAARIECRLETGRTHQIRVHLTEQTQTPIVGDPLYRPTHLPNELRAAQQL
                     IDHQALHARVLGFVHPTSQERLRFEAPWPADFEAALTALRQVS"
     gene            complement(2135..3124)
                     /locus_tag="VFQ61_31320"
     CDS             complement(2135..3124)
                     /locus_tag="VFQ61_31320"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET9959038.1"
                     /translation="MNRFVRRLLFATLLGVLVYGAFALYTGLARMQESLSAFQWSHFG
                     WALALASTNYLIRYLKWEYYLRRLGIRGVAAMDSLLVFLSGFVLTVTPGKVGEVFKSA
                     VLFRTHDVPVARTAPIVVAERLTDALGVVVLIVLGSTTFTGGGGWALAGGLAVATGVL
                     AIVWEAPVQATLRWLGRRGGKLAGLVPKLSEAYASLRVVAGPAALLIPTFLSCIAWGL
                     EGIALYLILRGFGADVPLPRAAFFYSTATLAGALVPLPGGLGIVEGIIREQLVHVSHM
                     QEGPATASMILIRFATLWWAVLVGFAALLVLRIRFKERLADGNALAEAGAGQG"
     gene            complement(3121..3345)
                     /locus_tag="VFQ61_31325"
     CDS             complement(3121..3345)
                     /locus_tag="VFQ61_31325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959039.1"
                     /translation="MLARHQPSAASARTSAAASASTASGTTVIVERPPPGLLRGRFAV
                     QPWVIGLLLGLVVLLVVGFYGTRLRRKLKP"
     gene            3486..4499
                     /locus_tag="VFQ61_31330"
     CDS             3486..4499
                     /locus_tag="VFQ61_31330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9959040.1"
                     /translation="MNDKFASTDVELLETCARACVRLREQVSRVVVGQQSVVDALLVT
                     LLARGHALLVGVPGLAKTLLVASIAEALGLSFGRVQFTPDLLPADITGTDVLNDAEGG
                     GGARPRVSFMPGPIFKNLVLADEINRTPPKTQAALLQAMQERTVTAGGVTHPLPDPFQ
                     VFATRNPIEQEGTYLLPEAQLDRFLLEIHVEYPSEKEEREIARRTTSGAKVTIEPVVH
                     AEEIRELGALVPRIPVTEDAVNLAVRMARVSRPSDPDAPEVVRKYVRFGAGPRGSQAL
                     VLAAKAHAALHGEAVADLDDVRDLALFALRHRLVLGYRADVDGVTDRDIVEAIVARAV
                     ENG"
     gene            4496..4765
                     /locus_tag="VFQ61_31335"
     CDS             4496..4765
                     /locus_tag="VFQ61_31335"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HET9959041.1"
                     /translation="MMMDLQALKQELKQLMIEQLDLRGKQPADIDDEAPLFGAGLGLD
                     SLDALQLAMAVEERFGVSVPEGEAAREVFASVESLANFVAGAKSA"
     gene            4797..7076
                     /locus_tag="VFQ61_31340"
     CDS             4797..7076
                     /locus_tag="VFQ61_31340"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET9959042.1"
                     /translation="MKQTSPRSELERDSSQSAHSPGRERVAITGVGMVTALGFGARPS
                     FARLCAGERGIRELEGFALPGLRSKFAAQVPSFDAPESYPRSDALAWLAAKEALAEAG
                     FGVGELPRMGLSVGGTTGSLLETEAELKQANGLARAQRFLTHPLSCTVQSLARLLGPF
                     AVGATSCSACSSGALAIVRGAQWVASGRTTLALVGGTDALCHMTLAGFGALGVVDPNP
                     CRPFDVKRAGLSLGEGAGFMVLEAESNARARGAKILGWLAGFAVGAEAHHVTHPEPSG
                     RRAAELMLSALGRAGFSAADVGYINAHGTGTQANDAMETRALEHAFGPELQRIWVSSS
                     KGQLGHTLGAAGAIEAVISLLALEAARVPPSAGLEHAEHPELRFVRERAADCAPGVVL
                     SNSFGFGGLDAVLCLSSDPPSNGIERAGGGEWLVRRSAWVPQSAAPGSNDALHAGAWL
                     ERARVLPDATDPFEGLDPERSRRFDRSAAALTRAAEQLGASRSAAEVARGLESEGVLA
                     RSGPEGSARGVLGRGLVVGAAYGNVERSLRFLDRLHAAGPKAVAPAEFPQLVPSASAG
                     SVSTYMGLTGPVFSVCDGIKSASLAVESALAMLELGLCSSLWCGAAATRDAVVRELFP
                     SVCPALQLAEGELSVLVELARRTPETEPGALARVVGHGACAGRHDADAIEPWAPPKPT
                     WPAHLFIADLDGSLGELVARSAWARVSQEDLLAGGFRETLGIHALALAAQAISREPSQ
                     ALILCGDRESAYWVRLEPL"
     gene            7073..>8317
                     /locus_tag="VFQ61_31345"
     CDS             7073..>8317
                     /locus_tag="VFQ61_31345"
                     /inference="COORDINATES: protein motif:HMM:NF014819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET9959043.1"
                     /translation="MIETHVVAAGAISALGSGRAAYRSPANAATKLRHDEELARSVAR
                     ANPGGNSLHASGTKTRPVLVGRVPDEYLTPSTGDRAEDLLRVAARELAVNLERALPDF
                     RSRRVLALVGTSAGGMPSLERALAARERAELTQELAERAGYCAPTRCLGPILGLPELR
                     IWSILAACASSTLAIGLGCRLLESGQYDLVIAGGYDALSPFVVSGFDALGALTRSVPR
                     PFSADRDGMALGEGAALLALALPSSLEQATARASALVGRVWGFGASSDAVHVTAPDPQ
                     GLGLSRAALTALADAGLPSSAMEWVSAHATATVLNDAAEAKALVRIFAGETPPISAWK
                     AVIGHTLGAAGGLECLAAWAASSERSLPTPSTALPTLEEASKSSVILQGGPCLKLSAA
                     FGGCNAALVLADAGAERIGPARS"
BASE COUNT         1394 a         2605 c         2846 g         1472 t
ORIGIN      
        1 tccggccgag tgccgcacgg agttcggcga agccggcaag gatcgctgtt tcggccgcgc
       61 ggggctgcgt gagctgatcg ctcaccctgg ccatttcctg gcgctaatcc cgcgcaagtt
      121 gtcggcaacc ttcgactggt ctggggctcc cggacactac ctaacggcct ccaattccag
      181 cgctttgagc gcggattctg cgctcttgct cggcatcgtg gaagccgtgg tgttgcggct
      241 ctcactgctg gcctgcttgt ggcgtgtcgg ggtcgcggac ggtcccagac ggcgcctacg
      301 tcgaagcgtg tgtttactgt cggcgccctg gttgttcctg aggccggctt ggatctccta
      361 cctcgggtta tgtatggccg gcctgagctt cggtcgcgac ctagatcgcc gcccgaacgt
      421 cgccttaggg gtggctgccg tggcttctac tgcgctggtc cacgcggtgt tttttggcgc
      481 ggggcgctac gggttggtgt gtgtcttggc gttacttccg ctcatcgccg agcgtcgcag
      541 ccataccctc gagccgaccg aagttacgct tcatgcccgc tgagcacttc tcgcttccgc
      601 ccagttttgc tgcgctgcga gtgcccgttg gaaggcgcgc gaccgtgcgg caggcggagc
      661 gctcaccgat cagcacatgg aggctgccat cggcacatct gtcatcggca gatctgtcac
      721 cggcagatct gtcatcggca gatagagtta ggtagaaggc accgaaaaca cgcgcgactt
      781 cacgcaaagc ggttttgaca gccggggccg ctcggggcga tacttcggtg tatgccgctt
      841 atcgaagtcg aggaagcagc gcgccgcttg gcccgggcga tcgctagcga cttgtccctg
      901 tataacgaag agaaaatcat ccgcgggatt gaaggtgact gcctgttcga ggaactggca
      961 gaggagatcg aggaagggcg cacgctatac aagagccgtg tctccccaga tctgtaccac
     1021 cgcaattatt acgatcgggc tctggtcgat atcctcgtga agtcgaaggg ccacatcaaa
     1081 agcaagattt ggtgactcgg ctcaggttcg tgctcggtcc cggagagcgc gagcggctcg
     1141 acaaggtgtt ggtgcgcttg tgcgaggggg cttcgcgggc caccgtccag cgttgggtgg
     1201 aggaaggccg agttcgcgtc aatggcaagg cgtgccgcgc gaaagaccaa gttggcccgg
     1261 gcgcggtgct cgaggtcgaa ccggcgccgc cgcctttgag cacggcagag cccgacgcgg
     1321 gggtgcgttt cgagctgctc tacgaagacg acgagctcct cgtcgtgaat aagccggcag
     1381 ggctcgtggt gcaccccgca cgcgggcatg ccaccggcac tctggtcaac ggcttactga
     1441 gtatgcagtc gtttcggagc gtgccggtcg atccgcgaga cccagcggga gtgctgcgcc
     1501 ccggcgtcgt gcaccgcatc gacaagggca cgagcggcgt gctggtgatc gcaaagaacg
     1561 aacgagttcg ggaaggcctg aaggagcagc tcgcgaagca cacggtggag cgcgtctatt
     1621 gcgcgctgac catcggtgtg ccccaagccg ggcgcatcgc gacgctttac gggcgtcacc
     1681 cgcagaaccg actgaagttc agctctcgcg tggctcaggg caaaaccgcg gtcacccacg
     1741 tttccgtttt ggagacgctc gcgggggggc gtgcggcacg gatcgagtgc cggctcgaga
     1801 ccgggcgcac gcatcaaatc cgggttcatc tgaccgaaca gacgcagaca cccatcgtgg
     1861 gcgatccgct gtatcgcccg acgcatctgc ctaacgagct gagggccgcg cagcaactga
     1921 tagatcatca agccctgcac gcgcgcgtgc tcgggttcgt gcaccccacg agccaagaac
     1981 gactacgctt cgaagcgcct tggcccgccg acttcgaagc cgccttgacg gcgttgcgcc
     2041 aagtttcttg agcccttcct acggcttgaa gtgctggcgc gtgagaacgc ggctctcgat
     2101 cgggctcgcg gcgagcgccg aacgtctcga ggctttagcc ttgtccggct ccggcttctg
     2161 ccagcgcgtt cccgtccgcc aggcgctctt tgaagcgaat gcgcagcacg agcaacgcgg
     2221 cgaagccaac gagcaccgcc caccacaaag tcgcgaagcg aatcaagatc atcgaggcgg
     2281 tggcagggcc ctcctgcatg tgactcacgt gcaccagctg ctcgcgaatg atgccttcga
     2341 cgatcccgag acccccgggg agcggcacca gcgcgccagc cagcgtggcc gtcgaataga
     2401 agaaggccgc gcggggcagg ggaacgtctg cgccaaaacc acgcaatatc aagtacagcg
     2461 cgatgccctc gagaccccac gcaatgcaag acaagaaagt agggatgagc agcgcggcgg
     2521 gccccgcaac cacacgcaag ctcgcataag cctcactcaa cttgggcacg agcccggcga
     2581 gcttgccgcc acgccgcccg agccagcgta gcgtggcttg gactggcgcc tcccaaacga
     2641 tcgccagcac tcccgtcgcg actgcgagcc caccggccag cgcccaaccg ccgccaccgg
     2701 tgaacgtcgt gctgccgagc acgatcagca ccaccacgcc caaggcatcc gtcaggcgtt
     2761 cagccacgac gatgggagcg gtgcgggcga ccggaacatc gtgcgtgcga aacagcacag
     2821 cgctcttgaa gacttcgccc accttaccgg gcgttaccgt cagcacgaag ccggacagga
     2881 aaaccaacag gctgtccatg gccgcgacgc cgcgaatgcc caagcgccgc aggtagtact
     2941 cccacttcaa ataccgaatt agatagttcg tactcgcgag cgccagcgcc cagccgaagt
     3001 gcgaccactg aaacgcgctc aagctttcct gcatgcgggc gaggcccgtg tacaaggcga
     3061 aagcaccgta aacgagcacg ccgagcagcg tggcgaatag aagacgtcga acgaagcgat
     3121 tcatggcttg agttttcgcc gcaggcgtgt gccatagaag ccaacgacca ggagcaccac
     3181 gagcccgagc agcagcccaa tgacccaggg ctggaccgcg aagcgtccgc gcagcagtcc
     3241 agggggcgga cgctcgacga tcaccgtagt ccccgacgcc gtacttgccg acgccgcagc
     3301 cgaggtgcgg gcactcgcgg cgctgggttg atgacgggcg agcatgcgcg cgaggcctta
     3361 cacgcgcgag cgagcgtgtg caagcggtgg ctcggccggc aagccgtctg gcgcgaaatc
     3421 actgcgctgc gagccgcgcg atctgctgca tgaaagctgc atgaaaaagg ctaagctgcg
     3481 acgcgatgaa cgataagttc gcttcgacag acgtggagct gctggagacc tgcgcgcgag
     3541 cctgcgtgcg cctgcgcgag caagtatcgc gcgtggtcgt ggggcagcag tccgtcgtgg
     3601 acgcgctgct cgtcacgctg ctcgcgcgcg ggcacgccct gctcgtgggc gtgcccgggt
     3661 tggcgaagac gttgctcgtt gcgagcattg ccgaggcgct cgggttgtct tttggtcgcg
     3721 tgcaattcac ccccgatctc ctgccggccg acatcactgg caccgacgtg ttgaacgacg
     3781 cggagggagg cggcggcgcg cggcctcgcg tcagcttcat gccgggaccg atcttcaaga
     3841 atttagtgct tgcggacgag atcaaccgca cgccgccgaa gacccaagcc gcgctgctgc
     3901 aggccatgca agagcgcacg gtgaccgcag gcggcgtcac ccacccgctg cccgatccat
     3961 tccaagtttt cgcgactcgg aacccgattg aacaagaggg tacgtatttg cttccggagg
     4021 cgcagctcga tcggtttttg ctcgagatcc acgttgagta tccgagcgag aaggaagagc
     4081 gggagatcgc gcgccgcacc acgtccggcg cgaaggtgac gatcgagccg gtcgtgcacg
     4141 ccgaagaaat tcgcgaactc ggcgcgctgg tgccacgcat cccggtcacc gaagacgccg
     4201 tcaacttggc ggtccgcatg gcgcgcgtgt cgcgccccag cgacccagac gcgcccgaag
     4261 tagtgcgaaa gtacgtaaga ttcggggcgg ggccgcgtgg cagtcaggca ctagtgctgg
     4321 ccgccaaagc gcatgctgct ctgcacggcg aagccgtggc ggatctcgac gatgtgcgcg
     4381 atttggcgct ctttgcgctg cgtcaccggc tggtgttggg ttatcgcgcc gacgtagatg
     4441 gcgtcacgga tcgagacatc gtggaagcca tcgtcgctcg cgccgtagaa aacggatgat
     4501 gatggatctg caagcgctca agcaggaact gaaacagctc atgatcgagc agctcgattt
     4561 gcgtggcaag cagccggcgg acatcgatga cgaggcgccg ctatttggcg cagggctcgg
     4621 cttggattcg ctcgacgcgc tgcagctcgc gatggcggtc gaagaacgct tcggggtgag
     4681 cgttccggaa ggggaagccg cgcgcgaggt ctttgcttcg gtcgaaagcc tggcaaactt
     4741 cgttgcaggc gcgaagagtg cctgaagcag cgcggctcgt tgcgagctct ggacccgtga
     4801 agcaaacctc tccccgatcc gaattggagc gcgactcttc ccagtcggca cactcgccag
     4861 gccgggaacg agtcgcgatt acgggcgtcg gtatggtgac tgcgctcggc ttcggcgcac
     4921 gccctagctt cgcgcggctt tgcgccggag agcgcggcat tcgcgaactc gagggcttcg
     4981 ctttgcccgg cttacgcagc aagttcgcgg ctcaggtccc gagtttcgat gcgccggaga
     5041 gctatccgcg cagtgatgcc ctggcctggc tcgcggcgaa agaagcactg gccgaggctg
     5101 gcttcggcgt cggtgaacta cctcggatgg gtctctccgt gggcggcacc accggcagtc
     5161 ttttggaaac cgaggcagag ctcaagcagg cgaatggtct cgctcgggcg cagcgttttc
     5221 tcactcatcc gctctcgtgc acggtgcaga gcttggcccg gctgcttggg cccttcgcgg
     5281 tcggagcgac gtcatgtagt gcgtgctcga gcggagcttt ggcgatcgtg cgcggtgcgc
     5341 aatgggtggc gagcggcaga actacgttgg ctttggtggg cggcaccgac gcgctctgtc
     5401 acatgactct cgcgggcttt ggtgcgctag gggtagtgga tccaaacccg tgccggccat
     5461 tcgacgtcaa acgcgcgggt ttgagcctcg gggagggggc cggcttcatg gtgctcgagg
     5521 ccgaatcgaa cgcccgcgct cgtggtgcga agatcttggg ttggttggcg ggcttcgccg
     5581 tgggagcgga ggcacaccac gtcacgcacc ccgaaccgag cgggcgacgt gcggccgagt
     5641 tgatgctcag cgccctgggg cgagcgggct tttctgccgc ggatgtgggc tatatcaacg
     5701 ctcacggcac cggcactcaa gccaacgacg cgatggagac gcgggcgctc gagcacgctt
     5761 tcggtcccga gttgcagcgc atttgggttt cttctagcaa agggcagctt gggcacactt
     5821 tgggtgcggc gggcgccatc gaggccgtaa tcagcttgct cgcgctcgag gccgcgcgcg
     5881 tgccaccgag cgcggggctc gaacacgctg aacacccgga gttgcgcttc gtgcgcgagc
     5941 gggccgcgga ttgtgctccg ggggtggtgc tctcgaactc gtttgggttc ggcggcttgg
     6001 atgccgttct gtgtttgagt tcggatccac cttccaacgg catcgagcgc gcaggtggcg
     6061 gtgagtggct ggtccgccgc agcgcttggg taccgcagag cgcggccccc ggctcgaacg
     6121 acgcgttgca cgcgggcgcg tggctggagc gcgcgagagt cctgcccgac gcaaccgatc
     6181 cgttcgaagg cctcgatcca gaacgctcac ggcgcttcga tcgctccgcc gctgcgctca
     6241 cccgagccgc tgagcaactc ggcgcgtccc gctccgccgc cgaagtggcg cgaggcctcg
     6301 agtccgaggg cgtgcttgcg cgatcggggc cagagggcag cgcgcgcggc gtgctcggcc
     6361 gcggcttggt ggtgggggcc gcgtatggca acgtcgaacg ctctctgcgc tttctggatc
     6421 ggctgcacgc ggccgggcca aaggccgtcg ctcccgctga gtttccgcag ctcgtgccgt
     6481 cggcctccgc gggcagcgtc tctacgtata tgggtctgac cggtccggta ttttcggttt
     6541 gtgatgggat caagagcgcg tctctggccg tcgagagcgc gctggccatg ctcgaactgg
     6601 ggctctgcag ctcgctttgg tgcggtgccg cggcgactcg ggacgcggta gtgcgcgaac
     6661 tcttcccgag cgtttgcccg gcactccagc tggcggaggg agaattgagt gtgctggtcg
     6721 agttggcacg acgaacgccg gaaacggaac cgggtgcctt ggcacgcgtg gtcggacatg
     6781 gcgcttgcgc gggtcggcat gacgccgatg cgattgaacc atgggcaccc cccaagccga
     6841 catggcctgc gcatcttttc atcgcagact tggacggtag tctcggtgag ttggttgcac
     6901 gcagcgcttg ggcacgcgta agccaagaag atctgttggc gggcggcttt cgcgagacgc
     6961 ttggaattca tgcattggcg ctcgcggcgc aagccatctc acgcgagccg agccaagcct
     7021 tgatcctgtg tggtgatcgc gaaagcgcct actgggtacg tttggagccc ctttgatcga
     7081 gacccacgtg gtggcagccg gtgcgatctc cgcgctcggc agcgggcgag cagcgtacag
     7141 gtcgccggcg aacgcagcga ccaagctccg tcacgatgag gagttagcgc gcagcgtcgc
     7201 tcgcgcgaac ccggggggga actctttgca cgcgagcggt acgaagactc ggccggtact
     7261 cgttgggcgc gtgcccgacg agtacctcac cccgagcacc ggagaccgcg ccgaagattt
     7321 gctgcgtgtc gccgcgcgcg aactcgccgt gaacttggag cgcgcgttgc cagactttag
     7381 atcgcggcga gtcctggcgc tcgtgggaac gtcggccggc ggcatgccca gcttggaacg
     7441 cgcgttggcc gcgcgtgaac gcgcggagct cactcaggaa ctggcggagc gcgccggcta
     7501 ctgcgctccc acccggtgtc taggtccaat cctcggtctg cccgagctgc ggatctggtc
     7561 cattctggcg gcttgcgcct cgagcacgct ggcgattggg ctcggctgtc ggctgctcga
     7621 atccggccag tacgatctgg tgattgctgg tggctatgac gcgctcagcc cgttcgtggt
     7681 ctcgggcttc gatgcgctgg gagcgctgac tcggagtgtc ccacgaccgt tcagcgcgga
     7741 ccgagacggc atggcgttgg gtgaaggtgc cgccttgttg gcgctcgctc tgccttcgtc
     7801 attggagcag gctacggcgc gcgcctcggc cctggtcggg cgcgtctggg gcttcggtgc
     7861 gagctcggat gccgtacacg taaccgcgcc cgatccgcag ggactcgggc tatctcgggc
     7921 ggctctcacc gcacttgcgg acgcaggctt gccgagcagc gcgatggagt gggtgagcgc
     7981 ccacgcgacg gcaactgtct tgaacgacgc cgccgaagcc aaggcgctgg ttcgtatctt
     8041 cgcgggtgag actccgccaa tatcggcttg gaaggccgtc atcggacaca cgctcggagc
     8101 tgcgggcggg ctcgagtgtt tggcggcctg ggccgcctcg tctgagcgct ctcttcctac
     8161 gccttccacc gcccttccca cgctcgaaga agcttctaag tcgagcgtca ttctgcaggg
     8221 cgggccttgc ttgaagctgt ccgccgcgtt cggcggttgc aatgcggctc ttgttttggc
     8281 ggatgcgggc gctgagcgca ttgggcctgc gcggtcg
//
LOCUS       DASTKU010000892         3317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1516138, whole genome shotgun sequence.
ACCESSION   DASTKU010000892 DASTKU010000000
VERSION     DASTKU010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516138"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFQ61_31350"
     CDS             complement(<1..797)
                     /locus_tag="VFQ61_31350"
                     /inference="COORDINATES: protein motif:HMM:NF018210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959044.1"
                     /translation="MASPSLVPKGVAQDGEAQGDAPAPGSEQAEHNGSADRTTRRRAS
                     KSRAASRRQEAREFGNSSRVAQGGASGQLDRASGRYLLIAVKRVLASEVGGRVKLMLA
                     AILVLLVGVSALNVVNSYVGRDLMTAIEQGHTAQFLSKAVLWASIFGVSTVVAVIARF
                     IEERLGLLWREWLTRTLVGNYLTGGQYLALKEHPYFGNPDQRIADDARNFTTTTLSFG
                     LMLLNAFFGIVGFSGVLWSISPALMLVAIGYAAFGSLATIALGRPLVR"
     gene            1615..>3317
                     /locus_tag="VFQ61_31355"
     CDS             1615..>3317
                     /locus_tag="VFQ61_31355"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9959045.1"
                     /translation="MTARHLSSRPFILVAAITACNGTITDSGGSGNAAQPPVSGGAAG
                     NGANPSPTTTPTSNPPTTPTGSASTVKLESAANYYRVVRLTNDQWTKSVQSVLALASA
                     PTDAENFQSAVAGTTDFTNNEQLLDVDSRAWTDYQAAAEGLAAKVSSDSALLSKLYSG
                     TDGAGFIKAVGRRIYRRPLTSGEQSTYQALFDSAAQFPGTQSAFAKGAGLVLETMLQS
                     PHFLYRTELGPAGSPLTPYEMAAKISLWLRDKTPDDALLDAAGGSGKYDTVDGAAALA
                     QKLLDDGASKAVMRKFHGEFLRFGKFSQLSKVGVANYDPSLNAELAESAYLFFDRIFS
                     SGQGVKDIFLSTKGFVSSRMATLYGGSSGPASGFEERELGANRVGYFTQLPFLILYGR
                     NSSPDSIHRGVNIALDVLCAPLGTFAGVIPPLPEVMPGQTNRMRVDEHTKGCGSGCHN
                     AMINPLGFAFENFDGMGAYRTTEKNGNESLAIDASGSFNFMDGPKSYKDSTELMQVLA
                     SSQQTHMCYSKRLASFGLQRNIVESDMPMLTRLATASANGSVKQVIVELVKQDAFRVR
                     AG"
BASE COUNT          653 a         1052 c          989 g          623 t
ORIGIN      
        1 cgcacgagag ggcgaccgag cgcaatcgtg gccaaggagc cgaaggcagc gtatccgatt
       61 gcgacgagca tcagcgccgg gctaatcgac cacagcactc ccgagaaccc aacgatgccg
      121 aagaatgcgt tgagcagcat caaaccgaag gagagcgtgg tcgtcgtaaa gttgcgcgca
      181 tcgtcggcga tgcgttgatc cggatttccg aagtacggat gctccttcag cgcaaggtac
      241 tgtccccccg tcaaatagtt ccccacgagc gtgcgcgtta gccactcgcg ccagagaagg
      301 ccgagtcgct cttcgatgaa gcgcgcgatc accgcgacca ccgtcgatac gccgaagatt
      361 gatgcccaca ggactgcttt cgaaaggaac tgcgctgtgt gtccttgctc gatggcggtc
      421 atgaggtcgc ggcccacgta gctattcact acgttcagtg cgctcactcc aacgaggagg
      481 accagaatcg ccgcgagcat cagcttgact cttccgccga cctccgacgc gagaaccctc
      541 ttcacggcaa tgagcagata cctgccgctg gcgcgatcga gctgcccgga agcaccgccc
      601 tgggctacgc ggctcgagtt cccaaactca cgagcctcct gccgcctgct tgccgcgcgg
      661 ctcttgctcg cgcgcctcct ggtggtgcgg tccgcacttc cgttgtgctc ggcctgctcg
      721 ctccccggcg cgggagcatc accctgcgcc tccccatcct gcgcgacgcc tttgggcacg
      781 agcgatggac tcgccatgta cggtttttag cagacggcga cgagcgactc ctgtcgggct
      841 cgagcgagtc aaaatgcccc acgcgcacag tgcacagcct cgcgcgcttg cagttgttat
      901 cgaagggttg ctgcaggctg ccccactttg caacggctgc cgacgccgaa cgcccaccag
      961 gctcgcacgt tacgcgcgct gtcgccgctc gaccaagtcc gaccgtacgg ttccagaagg
     1021 ttcccgtgtg gcgatgcgcg gcgacctggt ttcggcggac ggtgcaaagc gcgcgggcct
     1081 acttcccgct caggctttcg aggcatccgt tcatcgcact ttagaaaacg tacgcgcagc
     1141 ttgccgatgt tcagggaacc gcaggaaccg caactcgggt gatgatgata actgcgatgg
     1201 taggtcctcc tcgttcacca catctcgtga acggcaagac ttgatgcctc catcgcgagc
     1261 tgaagcgcac gcgaaagctt gctttcgact cgcgctgccg aagagtccgt tccgatgctt
     1321 tcatttgtgt ggttttcgag gcccaaactc aaataaaccg cacgccccta gctcgttccg
     1381 agacgtgatg aacggcaaag gtaagctcaa ttactttggt gtattagttt acttcagcat
     1441 ctaattcggt gtctttctcg gcgcgggcgc tgaaaataat gtgcgcggtt tcgtcgctta
     1501 cgtgacggat ctgcgttcga gaccgcactt cgatacagac gcgttgcaac gcgtgaagac
     1561 ggccgactcg tgcgggcgac ggcgcgggtg cgctcgcatc caagggcttc ctcgatgaca
     1621 gcacgacatc tcagttcgag accattcatc ctggtagccg cgatcaccgc gtgcaacggg
     1681 accatcacgg acagcggagg aagtggcaac gcagctcagc cacccgttag cgggggcgca
     1741 gctggaaacg gcgcaaatcc gagcccaacg accactccta catcgaatcc gccgaccacc
     1801 ccgacgggca gcgcgagcac cgtaaagctc gaaagcgcgg ccaactacta tcgcgtggtt
     1861 cgtctcacca acgatcagtg gaccaagagc gtgcagagcg ttctcgcgct cgcgtcggcg
     1921 ccgactgacg ctgaaaattt ccaatctgcc gtagccggca ccaccgactt caccaacaac
     1981 gagcagctgc tcgacgtcga tagccgcgcg tggaccgatt accaggcggc ggcggagggc
     2041 ttggccgcga aggtctcgtc tgattccgcg ctcctcagca agctttactc agggaccgac
     2101 ggcgccggtt tcatcaaggc agtcgggcgc cgcatctatc ggcggccact cacaagcggc
     2161 gagcagagca cgtatcaggc tctcttcgac tccgcggctc agtttccggg aacccaaagc
     2221 gcgtttgcga agggtgcggg cctcgtgctc gagacgatgc tgcagtcgcc acacttcctc
     2281 tatcgcaccg agttggggcc ggcaggctca ccgctcacgc cctacgagat ggctgcaaaa
     2341 atctcgctct ggctccgcga caagacgccg gacgatgcac tcttggatgc ggcgggcggt
     2401 agcggaaagt acgacaccgt ggacggcgcg gccgcgctcg cgcaaaagct actcgacgac
     2461 ggcgcttcca aagccgtgat gcgcaagttt cacggtgagt ttctgcgctt tggtaagttc
     2521 tcgcagctga gcaaggtcgg cgtcgcaaac tacgatccga gcctgaacgc cgagttggcg
     2581 gagagcgcgt acctgttctt cgatcgcatc ttcagcagcg gtcagggcgt aaaggacatc
     2641 tttctctcga ccaagggctt cgtgagctcg cgcatggcca ctctttatgg tggcagttcg
     2701 gggcccgcga gcggcttcga ggagagggag ctcggcgcca atcgcgtcgg ctactttacc
     2761 cagctcccgt ttttgatctt gtacgggcgc aactcttcgc ctgattcgat ccaccggggc
     2821 gtgaacattg cgctcgacgt gctgtgcgca ccgctcggga cgttcgcggg agtcattccg
     2881 cctcttcccg aggtgatgcc tggtcagacg aatcgcatgc gcgtcgacga acataccaag
     2941 ggttgcggca gtggctgcca caacgcgatg atcaatccgc tcggtttcgc cttcgaaaac
     3001 ttcgacggaa tgggcgccta ccgcacgacg gagaagaacg gcaacgagtc cttggccatc
     3061 gacgcgagtg gttccttcaa cttcatggac gggcccaagt cctacaagga ctcgaccgag
     3121 ttgatgcaag ttctcgcgtc cagtcaacaa acccacatgt gctactccaa acggctcgcg
     3181 agctttgggc tgcagcgaaa catcgtcgag tcggacatgc cgatgctgac gcggcttgcg
     3241 accgcgagcg cgaacggctc cgtaaaacaa gtgatcgtcg agctcgtgaa gcaagacgcg
     3301 tttcgggtgc gcgcggg
//
LOCUS       DASTKU010000893        17070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_620727, whole genome shotgun sequence.
ACCESSION   DASTKU010000893 DASTKU010000000
VERSION     DASTKU010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 17070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17070
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620727"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VFQ61_31360"
     CDS             complement(<1..874)
                     /locus_tag="VFQ61_31360"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET9959046.1"
                     /translation="MASCSLSRSRNVPPVPSQSERPSSGAWFGSLIQWLAALLALAWP
                     KAFVSHAGGRAFAGPESGHGWRAPGLMSFAAAALVLLGASTAAAAREVPPLAGRVNDT
                     AALLTQAERQRIEQKLAKYEADSGHQFAVLTIDTLDSDSLEEFSIRVVEAWKLGKKKV
                     DDGLLLLVVRDDRKIRIEVGYGLEGEIPDAIAARITRDTLKPAFRAGRYGEGIEAAID
                     ALIRATGREPAGGSAPVQRPVRGQPQGFPFPLVFIGLLILLPLLLSKLNSSAGGGRRY
                     RGGGGGVWPGGGFGG"
     gene            complement(915..1532)
                     /locus_tag="VFQ61_31365"
     CDS             complement(915..1532)
                     /locus_tag="VFQ61_31365"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET9959047.1"
                     /translation="MMNSHSIHERNWRSRWTQSLGALLLIGALSSCGYNEVIDRDEAV
                     KATWAEVQNQYQRRADLVPNLVKTVKAAGQFEQDTLTKVVEARSKVAGIKVDSSTIDD
                     PAKLKQFEQAQSELSGALSRLLVVSERYPELKATEGFRDLQAQLEGTENRITVARKRY
                     IDEVAEYNRVVQRFPTSIGAGMRGKAVRPTFEASKGSEKPPEVNF"
     gene            complement(1563..2384)
                     /locus_tag="VFQ61_31370"
     CDS             complement(1563..2384)
                     /locus_tag="VFQ61_31370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET9959048.1"
                     /translation="MDAIQEPPLFWVAHVDPMRARKSAPQTMAENSTPKNSQFERGDP
                     ESHPAAVRKHEERRRAALFDMDKTLLSANTAWLYTQHRRRQGEVGLRELARVSYWLLQ
                     YSMGIVDAERIARTALRDYAGRAASALAQTSDSWFKEHVRGYVRERARGVVEAHRRAG
                     DHLAIVTGATRYAAEPLARELGIDHVVCSELEVDSEGRLTGLPNDPICYGVGKLQRTE
                     QLASRLGFRIEEAAFYSDSITDLPLLERVGTPVAVCPDRRLRSLARRRGWRVEEW"
     gene            complement(2398..3027)
                     /locus_tag="VFQ61_31375"
     CDS             complement(2398..3027)
                     /locus_tag="VFQ61_31375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959049.1"
                     /translation="MWKLSSRTLSLSWLAMMMILPACGSEDEPASDDEKPSVWVGEVT
                     DSDALVGVAILGDDVDLFACGGPESISDLTVWLAGKKPVTRESPLTDGEFSVGFDVDA
                     SALRGDVANGDVSREFLVRPAEPGTLSGLYTADPAAVDGARLGLIVTQVPGESPMGQG
                     ALIRGVGQARIFEQVNPVFPAQLGDPIFVTLLSQPDTLIPVSRVVPGRQ"
     gene            complement(3303..4004)
                     /locus_tag="VFQ61_31380"
     CDS             complement(3303..4004)
                     /locus_tag="VFQ61_31380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9959050.1"
                     /translation="MTWFYLAHAAFHTLGISVPTLVDAVLGKSSLQAQDERLIAWSRR
                     VLNFAGVGLDVTYEAELSPDEAFVVMSNHQSLYDIPVIYSALQRRIRMVAKKELFSVP
                     IWGRAMREAGMVELDRGNHRAALASLDNARAAVARGISIWIAPEGTRSPDGRLANFKR
                     GGFRLAIATGARILPVTIDGTRNVLAAQSRIVHPNQSVRVVVHEPVATQGRTDSELSE
                     LVRLVQDKIQGGFRS"
     gene            complement(4063..5262)
                     /locus_tag="VFQ61_31385"
     CDS             complement(4063..5262)
                     /locus_tag="VFQ61_31385"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9959051.1"
                     /translation="MPAPAHLLPALETLISSGDFVVPPYPAAALRLRRLVEADDFSLV
                     EVTDAVSTDPALAAALLRLANSGAYASGSIPITTVGRAVHRVGARAVASLALATATAA
                     AACAPGPLIDLKYRVWRRSLSCALACQALAPRRTLDAQEAFLAGLLHGFGRSVVVGCL
                     ERVLPSKPVAEARPLLDWLAAVEPHRHTLALKVAQAWKLPPELVRAVAGQSAPNAAAA
                     EPMTWLVDLADRIGGALDRGETAEQVAATPGIRDDERKLITDFALSLPLSLDALLNPP
                     DARELSKRPRAMSAVSKPPSSFAGELRPLKLPVFDVRNRKQPRPMQCTAATPDGLVLL
                     GPDPMQESCVVRLSIGNPPEAVEGWFSVALCLPERELFRIEVQAFAASRELKETLTRL
                     WTSARDR"
     gene            complement(5371..6555)
                     /locus_tag="VFQ61_31390"
     CDS             complement(5371..6555)
                     /locus_tag="VFQ61_31390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959052.1"
                     /translation="MTLRWILPCGLWVLVSACGSSGEPNSVGTGGGPSGGNSATGSTD
                     SPVHGGGETAASGGSEAQGGQSSGSGGATQSSCSTDSDSCPDGSYCDGASCVQGCKAA
                     TDCQSGVCGDDHSCESCQSDSECSEGLLCSSGTCLEPCEEDGDSCGDGRTCCGGRCVD
                     TARDIQHCGACGGKGSDATCSSAEFCGGESCVPLTLASICEHTEVLLMEDEFEADGEA
                     GKTIAMALAAHCPSKPKVKVVPQGEATASNPMTGFPSAGPTRLVTLGGGPYGQLVAKY
                     LEDNSIPPVRHGFVDGVYRFEKIKGGEVLASMPWDQADGTKDIVVVELTREPNNGTTV
                     LIVSGFSAQGTQAGAWYFANVMLPQIDRYSAPYYVLNWTDGGENQSPDDSDQFQVIGS
                     AP"
     gene            complement(7034..8473)
                     /locus_tag="VFQ61_31395"
     CDS             complement(7034..8473)
                     /locus_tag="VFQ61_31395"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9959053.1"
                     /translation="MIQMRSERGLVRFAGLASGLVATRRAKRHLGFALLCMACGRIEL
                     DPGRSGSGGHGAASDMPSAGEASQAGRAAGGRTAEGGAHGGHAAGGVVSVGGRSNPVG
                     SGGTSAPDNTDGGTAAGGRSTQTDAGASAVSGRSSGGGSSTGTTEPLPSFGGAAGESS
                     GGTDSGAGGSATGGGPQSGFPTFPSCTSLSARCGATGDNCCSSLFVDSSSEELMFGAG
                     VRETPISTRSSSFYLDKYEVTVGRFQRFVAGYDAFRNSERLHEDAGQLPGIDGSGWQR
                     AWTDALPENSGALVDRVQSCNETPFATYSDQPEDATYPMNCVSWLEAMAFCIWDGGRL
                     PSQVEWEFAATGGALMRPYPWGDREPSSELAVFGCSDEGSHFTDCFNATIAPVGSKPA
                     GVGRFGHLDLAGSMFEWALDGPGLGDKGPFCLNCFNIFDGLVRAIRGGAFDSAGPDLL
                     AAKDQPRDLSGHHFDVGFRCARSSSRPIE"
     gene            complement(8470..9666)
                     /locus_tag="VFQ61_31400"
     CDS             complement(8470..9666)
                     /locus_tag="VFQ61_31400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959054.1"
                     /translation="MSYGAPGQGPPRLADDPRFSALFREADRSFAESRDEIRDFARLR
                     SKLGNGRRLSGFTVYGRWAMVGVVALVGFIAVRRAVAPAPVEVARENVWRPRSAADRA
                     PKDSRVDGRGSEAPRASEVVDADGMHTREDRASEDARAASGRVAKKASERAPSSELAG
                     NGLVGNFEQPASDESANSRPPLVAQKARLGAGEAAQKPDSRNPPSSSASAPDCLSFAR
                     EGRPQDADACFMQRGAGSGLAAEVSLYEAARLRRDVLGDPQAALQTLSLYRTRFPSGS
                     LRSEVDMSYLELLSRAGRDAEALAESARLLQSVAGNERFVELHALRGNIFRKGKDLRA
                     AEREYGFAERGSGALAREAAYLRGTCLQSLGEDSAARAAFERYLAKGAGPRERDIRQR
                     LLRLVP"
     gene            complement(9663..10514)
                     /locus_tag="VFQ61_31405"
     CDS             complement(9663..10514)
                     /locus_tag="VFQ61_31405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9959055.1"
                     /translation="MVRAAVTPLAELPTCAEPPLVLDCVRGDRSAWRRLHQRYHPVSV
                     AFLRRLGVAEPDLEDATQEVFLEMFRYLPGFRGSSELTTWLYRLCITQARRARRRRRV
                     AQALQQLLLMAPAEHLISSPAFSENQARKRIEGALSRLSDAERSVFVLYEMEGLSGKQ
                     IAEILGCKEATLWRRLHYARKRFIEVFDPATQSESSTQAGSSTEASWSVATSNEPSAH
                     ELSPQDSAGAPIDSPQRGNGAERINGAHPFNGAERINGAERINGAERINRAERINGAE
                     RINGAER"
     gene            complement(10702..11712)
                     /locus_tag="VFQ61_31410"
     CDS             complement(10702..11712)
                     /locus_tag="VFQ61_31410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959056.1"
                     /translation="MLSACLFCSRMAFGEENAGLTLESGSPDALCPDLTRARAAVERR
                     LGRFEFPSGPAWKARYTLGHRPEGEPRDFVQLELFDGQGQLKLRRELPTANESCVTLA
                     EVIALVLDRYFRALSGEEGGLAPLAEGVPETNSRSPEPREREPVDPVQRDSSTVQLGS
                     QTSPRTAAPVRAMLGASLGVSSSGADAHLTLKGLFAAFPGSYWSASLRTPLARRFESL
                     SEDRYVSTRELVAGGSLGLVGDSAGFRLFAGPTLGLGVERASGVFRESSAKYRAMWSL
                     GAEAGAIYKPGGRLLAFAHGALELPLARWSGQFYVDDREVLRLPRVQALFSLGLGVEL
                     SN"
     gene            complement(11944..14010)
                     /locus_tag="VFQ61_31415"
     CDS             complement(11944..14010)
                     /locus_tag="VFQ61_31415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959057.1"
                     /translation="MAMLAACSAPSVLKPTPKGPEPKAGPPLQGQTSNGVPASEHFNR
                     LDRDEFNQRAVLWNVPVFWRKDAGALGALDPEELAILFGAEQHARAEFVNPAAQFTPR
                     FADIYERLQTDPPIPPNAAPAEVQRRESVRLELLQGRPTLVDTDFSKASAGERALVAH
                     VLNAARGIERLYARQRGTLGLEAGLAADDPESAALYFRNQGPTCVAPKTEKLASCNAL
                     PSGNKPSFGLYPSNIQTNEAIANASLPGWCRLLEQQPNADALKDHFSVVEAAGTGFRA
                     TPYHVAYREDMEAVARELEQAEAALGQEEPALRRYLSAASAAFRTNQWEPANEAWVAM
                     GSDNSRYYLRVAPDEVYYEPCAWKAGFALTFARINDTSKQWRDKLDPLKQELETELAS
                     LAGKPYVARQVGFKLPDFIDIVLNAGDSRHPHGSTVGQSLPNWGPVAERGGRTVMMAN
                     LYTDWDSRRSFERQAASLFCKASYARVTSSEEPAVLGVVLHEAAHNLGPAHEYKVAGR
                     VDHAAFGGALAATLEELKAQSAALYLPHWLVSRGALPSELVQQALLREIAWAFGHIAQ
                     GMYDAAGRPKNYSQLAAIQLGVLQDAKLVIWRPTELARNGEDMGCFDVDFARAETATR
                     ELARLVLQIKARGDRARALTLVARYVDGKDAFKTLKDTITERWLRVPKPSFVYSIQGA
                     GPQELL"
     gene            14734..15261
                     /locus_tag="VFQ61_31420"
     CDS             14734..15261
                     /locus_tag="VFQ61_31420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959058.1"
                     /translation="MKGPAATELEPRGPGVMLAVGPTFGLLPQPAWGVSLEGSWRTGR
                     WGGALSLRYLAPVEKEYRAPVELHAQAFGAHLGAWFEPWPELRLHAGGVTYAVLGRGV
                     GSQVEGSGRVWAAGPVLGALAVPARTRHLWVGVGAELHVEVVRPSFEIVPYEQIFRSS
                     LAVGSVFLAIAPVFR"
     gene            15295..15885
                     /locus_tag="VFQ61_31425"
     CDS             15295..15885
                     /locus_tag="VFQ61_31425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9959059.1"
                     /translation="MATGTLDSRALTPQAAPGEHAVRAPGIQEVYAEHFRYVWRCLRS
                     LGVRDAVLDDALQDVFLVVQRKLASFEGKSELRTWLYAIALRVARRYRTRPKEEPGPE
                     ADGEHAAIDAEACANLEASVECNQRLELARRALDALHDHKREVFVLAQIEQMSAPEIA
                     EITGLPLNTVYSRLRAARLAFSAEVRRLEELPKRLP"
     gene            15882..16865
                     /locus_tag="VFQ61_31430"
     CDS             15882..16865
                     /locus_tag="VFQ61_31430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959060.1"
                     /translation="MKPESSIDELVDALRADLPSPRDEARVRARLAAVGVSVGAGVTA
                     GGAAAAPITKALLPGAKLAAKLYALPLTIKASVVGVASVSAVVTPLWLASQAREMHRA
                     APSTVSSSSALSKAAVTRQANGDVLASKPTPAPPATQPPTDSPPDRSPKLTQSSPEPK
                     RPLGAPRAPSVALEPRSRSGLRSANEEPAALLPRSPGNSAATHAVIAVSRSSVPVPTT
                     RPDVTAPSSITAEPTQAIGTATFAQVEPAAAPSPVQAPKLTTASGSESSLREETALVD
                     TALIALRDGDRSKARLLLETHARRFPHGLLARERERARRKLLQASGSDSTH"
BASE COUNT         3198 a         5691 c         5162 g         3019 t
ORIGIN      
        1 ccccgccgaa accacctccc ggccagactc cccctccccc accgcgatac cgccgacccc
       61 cacccgccga ggaattcaac ttactcagca gcaaaggcag caaaatcagc aacccgatga
      121 acacgagcgg gaacggaaaa ccctgaggct ggccgcgcac cggtcgttgc acgggcgccg
      181 aaccacccgc gggctctcgc ccggtagccc gaatcagcgc gtcgatcgcg gcctcgatgc
      241 cctcaccgta gcggcccgct cggaaggcgg gcttcaaagt gtcgcgggta atgcgcgccg
      301 caatcgcgtc cgggatttcg ccttccaggc cgtagccgac ctcgatccgt atttttcgat
      361 catcgcgcac gaccagcagc aaaagtccgt catcgacctt tttcttgccg agcttccaag
      421 cttcgacgac gcgaatcgag aactcctcca ggctgtccga gtcgagggta tcgatcgtca
      481 gcaccgcgaa ttggtgaccc gagtcggctt cgtacttcgc gagcttctgc tcgatgcgtt
      541 gacgctcggc ctgcgtcagg agcgcggcgg tatcgtttac gcggccagcg agaggcggaa
      601 cttcgcgtgc cgcggccgcc gtcgaggccc cgagcagtac cagagccgcg gccgcaaagc
      661 tcatcagacc aggcgcacgc cagccgtgcc ccgattccgg ccccgcgaag gctctccccc
      721 cggcgtgcga gacgaatgct ttcggccaag ccaatgccag caacgccgcc agccattgga
      781 tcaaagaccc gaaccaagcg ccggagctcg ggcgctccga ttggctcggg accgggggaa
      841 cgttccgcga gcgggagagc gagcacgaag ccatcgctct cgggtgatgc aaggcggcgt
      901 gaagcattcg ccgatcagaa gttgacttcg ggcggttttt cgctgccctt cgaggcctcg
      961 aacgtggggc gcaccgcctt gccgcgcatg ccagcgccga tgctggtcgg gaagcgctgg
     1021 accacacggt tatattcggc gacctcgtcg atgtaacgct tgcgtgccac cgtgattcgg
     1081 ttctcggtgc cctcgagctg ggcctggaga tcgcgaaaac cctcggtggc cttgagttcg
     1141 ggataacgct cggaaaccac gagcagccgc gacagcgcgc ccgaaagctc ggattgtgcc
     1201 tgctcgaact gcttgagctt ggctggatca tcgatggtcg agctatcgac cttgataccc
     1261 gccactttgg agcgcgcctc gacaaccttg gtgagcgtgt cttgttcgaa ctgaccggcg
     1321 gctttgacgg tcttgaccag gttaggaacc aaatcggccc gacgttggta ctggttctgc
     1381 acctcggccc aggttgcctt cacggcttcg tcccggtcga tcacctcgtt gtaaccgcag
     1441 ctagagagcg ccccgatcaa cagcagggcg cccagactct gggtccaacg actgcgccag
     1501 ttccgctcgt ggattgaatg cgagttcatc atttcgattt gtccctgcgt actttgctcg
     1561 gcctaccact cttcgacgcg ccagccacgc cgccgcgcca aactcctcag ccggcgatcg
     1621 gggcaaacgg ccaccggcgt tccgacgcgt tcgagtagcg gcagatccgt gatgctgtcc
     1681 gagtaaaaag cggcttcttc gatgcggaaa ccaagacgcg atgcgagttg ttccgtgcgc
     1741 tgcaatttcc caacgccgta acagatgggg tcgttgggca gaccggtcaa gcggccctcg
     1801 gaatccacct cgagctcgct acacaccacg tggtcgattc cgagctcacg ggccagcggt
     1861 tcggcggcat atcgcgtagc tccggttacg atcgccaagt ggtcccccgc gcggcggtga
     1921 gcctcgacga ccccgcgggc tcgctcacgc acgtaaccac gcacgtgttc cttgaaccac
     1981 gagtcgctcg tctgcgcgag agcactcgcc gcacgccccg cgtagtcgcg cagagccgtg
     2041 cgcgcgatgc gctcggcatc gacgataccc atcgagtatt gcagcaacca gtaactcacg
     2101 cgcgccagct cgcgcagccc cacctctccc tgccggcgcc gatgttgcgt gtaaagccag
     2161 gccgtgttcg ccgacagcaa cgtcttgtcc atgtcgaaca gggcggcccg ccgacgttcc
     2221 tcgtgttttc gcacggccgc agggtgcgat tcggggtcgc ctcgttcgaa ctggctgttc
     2281 tttggagtgc tgttttcggc catggtttgc ggagctgact tgcgggcgcg catcgggtcc
     2341 acgtgagcga cccagaagag cgggggctcc tggatcgcgt ccacgccgct atcggcccta
     2401 ttgccgcccg gggacgaccc tgcttacggg aattagcgtg tcaggctgcg agagcaaggt
     2461 cacgaagatg ggatccccga gctgagccgg gaaaacgggg ttcacttgtt cgaaaatgcg
     2521 tgcctgtccc acgcctcgaa tgagcgcgcc ttgtcccatg gggctttctc caggaacttg
     2581 ggtgacgatc aaccccaaac gcgcgccatc caccgccgcc ggatccgccg tatacaaccc
     2641 cgacagagtt cccggttctg cgggccgaac caggaattcg cggctgacat cgccgttggc
     2701 cacatctccc cgcagggcgc tcgcgtcgac gtcgaagccg accgagaact cgccatcggt
     2761 gagcggactt tcgcgagtga ctggcttctt gcccgcgagc catacggtga ggtccgagat
     2821 ggactccggg cctccacatg caaacaggtc aacgtcgtct ccgagtatgg ccacgcccac
     2881 caacgcatcc gaatcggtca cctcaccgac ccaaacgctg ggcttctcat cgtcactcgc
     2941 gggctcgtct tccgagccac acgcgggcag aatcatcatc atcgcgagcc agctcagcga
     3001 gagcgttctc gaacttagct tccacatggt cgttcctctt cgtgcgtcgc cactcacgag
     3061 catgggcttc taatccgaag tcctcgtcga aacagcccgg agcggctcga acaagccacg
     3121 ctgcagacgc catgccaccc tcttgccgga ttcaaacacc agcaaataca gagatttacg
     3181 atttagaacg gcaggttttg tagcggcacg tgcccggcac cggccagtga agaaagctca
     3241 catgcgattg catgtcgctc gcagcgagcg acacgcttgc atcgacgtcg atactgcgcg
     3301 gctcagctcc ggaacccgcc ctgaatcttg tcttggacga gccgaaccaa ctcagacagc
     3361 tcggaatccg tacgcccctg cgtggccact ggctcgtgca cgaccacgcg cacgctctga
     3421 ttgggatgga cgatgcgact ctgagccgcg agcacgtttc gagttccgtc gatcgtgacc
     3481 ggcagaattc tcgcgccggt cgcgatcgcg aggcgaaagc cgccccgctt gaagttggcg
     3541 agccgcccat cggggctgcg cgtgccctcg ggggcaatcc atatgctgat accgcgcgct
     3601 acggcggcgc gagcattgtc gagactggca agcgcggccc ggtgattgcc acgatccagc
     3661 tcgaccatgc ctgcctcgcg catcgctcgt ccccaaatcg ggaccgagaa cagctccttt
     3721 ttcgcaacca tgcggatgcg gcgctgaagc gcggaataga tgaccggaat gtcatacaga
     3781 gattggtggt tcgacatcac gacgaaggcc tcatccggag agagctccgc ttcgtaggtg
     3841 acatcgagcc ctacgcccgc aaagttcagg actcgccgag accaggcgat caagcgctca
     3901 tcctgcgctt gcaggctgct cttgccgagg accgcatcaa ccagcgttgg aactgaaatt
     3961 cccaacgtgt ggaacgccgc gtgggccagg taaaaccagg tcaaaactca gccaccaaag
     4021 cacccctgat ctggggccgc aactgagttc cggtcgggtg tgtcagcgat cacgtgccga
     4081 ggtccagagc cgggtcaacg tctcttttag ctcccgactg gcggcgaacg cttgcacctc
     4141 gattcggaag agctctcgct cgggaaggca caaggccacg ctgaaccaac cctccaccgc
     4201 ttcaggcgga ttgccgatcg aaagccttac cacgcagctc tcctgcatcg ggtcggggcc
     4261 cagcaatacc aatccatcag gggttgcggc agtacactgc atcggccggg gctgcttccg
     4321 gttccgcacg tcgaacacgg gcaacttgag cggccgcagt tcacccgcga atgaactcgg
     4381 gggcttgctg accgcgctca tcgcgcgggg acgtttgctc agctcgcgag catcgggcgg
     4441 gttcagtaga gcatccagcg agagcggcaa actcaaggca aagtcggtga tcagcttgcg
     4501 ctcgtcatcg cgaattcctg gcgtcgcggc gacctgttcg gcagtttcac cgcggtcgag
     4561 agctccgcca attcggtcgg ccaagtctac gagccaagtc atgggttcgg cagcagccgc
     4621 gttgggagcc gattgccccg cgacggcgcg cacgagctcc ggaggcaact tccaagcttg
     4681 tgcgactttg agggccaacg tgtgacgatg aggttcaacg gcggcgagcc agtcgagcaa
     4741 ggggcgagct tcggctacgg gcttcgaagg cagcactcgc tccaaacagc cgaccacgac
     4801 tgatcgccca aatccgtgca gtaaccccgc cagaaacgcc tcttgagcgt ccagggtacg
     4861 gcgcggtgcg agcgcctgac aggctaaagc gcacgacaag ctgcgacgcc acacccgata
     4921 cttcaagtcg atcagggggc ccggcgcaca agccgccgcc gccgtagccg tggccagtgc
     4981 caacgacgcc acggcgcgag cgcccacccg atgcacggcg cgtcccaccg tcgtgatggg
     5041 gatacttccg gaagcgtagg caccggagtt ggcgagtcgc aacaaggccg ccgccaacgc
     5101 gggatccgtc gagaccgcgt ccgttacttc caccaagctg aagtcatccg cctcgaccaa
     5161 gcgccgcaga cgcaaggccg ccgcaggata gggtgggacg acgaagtccc ccgatgaaat
     5221 gagggtctcg agcgccggca aaaggtgagc aggtgcgggc atggtcaaac gctttcaccc
     5281 ggctcgagct cgtcactctc ggaacgcgcg gacgtggaac acagggcgcc aactgcgggc
     5341 accctgtgcg tccatcttca ggtgtaagac ttacggagca gagccgatca cttggaattg
     5401 gtcagagtca tctggactct ggttctcgcc gccatcggtc cagttgagaa catagtacgg
     5461 cgcgctgtag cgatcgattt ggggcaacat cacatttgcg aaataccaag cgccggcctg
     5521 cgtgccttgt gcgctgaagc ccgacacgat caagactgtg gtgccgttgt tcggttcgcg
     5581 agtcagctcg accaccacga tatccttggt cccgtcagct tgatcccaag gcatgcttgc
     5641 caaaacctcg ccgcctttga tcttctcgaa gcgatacact ccgtcgacga aaccgtggcg
     5701 cacgggagga atcgagttgt cctctagata cttggcaacg agctgcccgt aaggcccgcc
     5761 tccaagagtg acgagtcgcg tagggcccgc cgagggaaag ccagtcatgg gattggaggc
     5821 cgtggcttcg ccttgtggca cgactttgac tttgggcttc gacgggcaat gcgccgcgag
     5881 cgccatggcg atcgtcttgc cggcttcccc gtcggcttcg aactcgtcct ccatgaggag
     5941 cacttccgtg tgttcgcaga tgctggccaa cgtgagcgga acgcagctct cgccgccgca
     6001 gaattcagcg ctagagcagg tcgcatccga gcccttgccg ccacaggcac cgcagtgctg
     6061 gatgtctcgc gccgtgtcta cacagcggcc gccgcaacac gtgcggccgt caccgcaact
     6121 gtcgccgtcc tcttcacagg gctccaagca agtgccactg gagcacaaca acccttcgct
     6181 acactccgaa tcgctctggc agctctcgca gctatggtcg tcgccgcaca cgccactttg
     6241 gcagtccgtg gcggccttgc agccctgcac gcagctagca ccgtcgcagt atgagccatc
     6301 cggacaacta tccgaatcgg tagaacagct gctctgggtg gcgccgcccg aaccagagga
     6361 ttggccgcct tgggcttcgg agccgccgct cgccgcggtc tctccgcctc cgtgtacagg
     6421 ggaatcggtg cttcccgtcg cagaattgcc gccggaaggg ccaccgcccg ttcccacgct
     6481 gttgggttca ccgctagaac cgcacgcgct gactaaaacc cacaaaccac aaggcaagat
     6541 ccaccgcaac gtcatgacga ctcctttgcg tgcgcggctt cacgagcgca cacgcgaggt
     6601 ctcaagcagg ctggttccgc agaaccgagt tcattcgaat cgcggcacgt ggagtccacg
     6661 gccggtctac tgacttcggc aatgcggtta tgcagcagct gtgccgtggc gctctcgcac
     6721 tcgactggca cctgcgacac ccgatggggc tggtgaagca cacctagtag agtagcagcg
     6781 agatcatttt gcgccgcctc ggagtggagc gatgtgcaat cgcggcacgc ggcaactttg
     6841 cggtgatcac gaatcactcc ccgtggatag tgactctagt catctcgcgc tgaatgtttg
     6901 cgaacggttg gagctttctc gctaggcagc ttcgttggtt gatgtcgtcg cctcgaggaa
     6961 gaaaccccga ctcgatacgt ctacctgagc cgtctggctg actcacgcgc ttggggggtc
     7021 gagatgggca ggctcattcg ataggacgag aggagctccg cgcacagcgg aatccaacgt
     7081 cgaaatgatg cccggagaga tcccgaggtt gatccttggc cgccagcaag tctgggcccg
     7141 ccgaatcaaa cgctccgccg cgaatcgccc gcaccaggcc gtcgaagatg ttaaagcagt
     7201 tcaagcaaaa cgggccttta tcccctagcc ctggaccatc gagcgcccac tcgaacatcg
     7261 agcccgcaag atcgaggtgc ccgaagcgcc caaccccagc tggcttagaa cctacagggg
     7321 cgatggtggc attgaagcaa tcggtgaagt gggaaccttc atcggaacag ccgaacaccg
     7381 ctagttctga ggagggctct cgatcgcccc atggataagg gcgcatcagc gcgccgcccg
     7441 tggcagcaaa ctcccactcc acttgactcg gcaggcgccc cccatcccag atgcagaagg
     7501 ccatcgcctc gagccaactg acgcaattca tcggatacgt cgcgtcttcc ggctggtccg
     7561 agtaggttgc gaaaggagtt tcattgcacg actgcacgcg gtccacgagg gcgcccgagt
     7621 tctcgggcag agcgtccgtc catgccctct gccatccgct cccgtctatt cccggcaact
     7681 gccccgcgtc ttcgtgcaag cgctcgctat tgcggaaggc gtcgtagcct gcgacgaagc
     7741 gctggaatcg ccccaccgtg acttcgtatt tatccaagta gaagctcgag ctgcgcgtgg
     7801 aaatcggcgt ctcacgtacc ccggcaccga acatcagctc ttctgagctc gagtcgacga
     7861 acaagctcga gcagcagttg tctccggtgg ccccgcagcg cgcagacagc gacgtgcacg
     7921 aggggaacgt gggaaaaccg gactgcggcc ctccccccgt tgcagagcct cccgcgcccg
     7981 agtccgtgcc acccgagctc tcgcccgcgg cgccgccaaa gctcggcaac ggctcggtcg
     8041 tgccggtaga gctaccacct ccagagctgc gaccggaaac cgccgaagcc cccgcatcgg
     8101 tttgagtgga gcgtccgccg gcagctgttc cgccatcagt attatcaggc gccgacgtgc
     8161 ctcctgaccc aactgggttg gatcgcccac caacagagac caccccaccc gctgcatgac
     8221 cgccgtgagc accgccctcc gcggtacgcc caccggcggc ccttccagcc tgtgaagctt
     8281 cacccgctga tggcatatcc gacgctgcac cgtgcccccc actcccagag cgaccggggt
     8341 cgagctcgat ccggccacat gccatgcaca acaacgcgaa ccccagatga cgcttagcgc
     8401 gcctggtcgc gacaaggccg gaagccaagc ctgcaaaacg cacgaggcca cgttcggagc
     8461 gcatctggat catgggacca accgtagaag tctttggcga atgtcgcgct cacgcggccc
     8521 cgcacctttg gctagataac gttcgaacgc agctctggcg gccgaatctt ctccgagaga
     8581 ctgaaggcaa gtaccccgta gataggccgc ctcccgcgct aacgcgcccg acccacgctc
     8641 tgcgaaaccg tactcccgct ccgccgcgcg gaggtctttg cctttgcgga agatattgcc
     8701 acgcaacgca tgcagctcga cgaagcgctc gttgcccgcc acggactgga ggagccgcgc
     8761 actctcagcg agagcctcag cgtcccgccc ggcacggctc aacaattcga gatacgacat
     8821 atccacttcg gaccggaggg agcctgacgg aaaacgcgtg cgatacagac tcaaggtttg
     8881 cagcgcggct tgggggtcgc ccagtacatc cctccgcagc cgagccgctt cgtatagcga
     8941 gacctcggcc gctagccctg agcccgcacc tcgttgcatg aagcaggcgt ccgcgtcctg
     9001 tggacgccct tctcgcgcga atgaaaggca atctggcgcg gaagccgacg agctcggtgg
     9061 atttctcgaa tctggcttct gggctgcctc accggccccg agtcgagcct tctgagccac
     9121 gagcggcgga cgcgagttcg cagactcatc actagccggc tgctcgaagt tacctactag
     9181 gccgttacca gccagctcgc tcgacggcgc gcgctcggat gccttcttcg ccacccgtcc
     9241 ggaagccgcg cgcgcgtcct cactcgctct gtcctcgcga gtgtgcatgc cgtcagcgtc
     9301 gacgacctcg ctcgctcgtg gagcttcaga gcctcgcccg tccacgcgcg aatccttcgg
     9361 agcccgatcc gctgccgatc gtggccgcca cacgttctca cgcgcaactt cgaccggcgc
     9421 tggcgctact gctctccgca ccgcgatgaa gcccacgagg gccaccacgc cgaccatggc
     9481 ccatctgccg taaacagtaa aaccgctgag ccgtctgcca ttccccagct tgcttcttag
     9541 ccgagcaaag tctcggattt catcccgcga ttcggcgaag ctgcgatctg cctcgcggaa
     9601 tagcgccgag aagcgcggat cgtctgcgag gcgcggaggc ccttgaccgg gggcgccgta
     9661 gctcatcgct ccgctccgtt gattcgctcc gctccgttaa ttcgctccgc tctgttgatt
     9721 cgctccgcgc cgttgattcg ctccgcgccg ttgatccgct ccgcgccgtt gaatgggtgc
     9781 gctccgttga ttcgctccgc gccgttgccc cgctgagggc tatcgatcgg tgcacctgcg
     9841 ctgtcttgcg ggctgagctc gtgggcgctg ggctcgttcg atgtcgcgac gctccagctg
     9901 gcttcggtcg agctgccggc ttgcgtggac gactccgact gagttgccgg atcgaacacc
     9961 tcgatgaaac gcttgcgcgc gtagtgcagt cgcctccaga gcgtggcttc tttgcagccc
    10021 aagatctcgg cgatctgttt gcccgaaagc ccttccatct cgtaaagcac gaagacgctg
    10081 cgctcggcgt cggacaggcg actcaacgcc ccctcgatgc gtttacgggc ttggttctcc
    10141 gagaacgcgg ggctcgagat caggtgttcg gcaggcgcca tcagcagcaa ttgctgcaac
    10201 gcctgagcga cacgacgccg acggcgcgca cgacgagctt gcgtgatgca caatcgatac
    10261 agccacgtag tgagttcgga gctgccccga aatccgggca gataacggaa catctccaga
    10321 aatacttcct gggtggcgtc ctccagatcc ggctctgcta ctcccaaacg ccgcaagaaa
    10381 gcgacggaaa cagggtgata acgttgatgc agccggcgcc acgcggagcg atctccacgg
    10441 acgcaatcga gcaccaacgg aggctccgca cacgtgggga gctcagccaa gggcgtgacg
    10501 gcagcgcgca ccataccttc ctcttagaga cgcttgctct ccaaaccttc acttcgaaaa
    10561 ctactcatcg caattttcga tgtttttcgc ccagttcccg ctgcggtttc gctaacttgc
    10621 ctcgcctcca ctcggcactc ggccttgcgg aagcctcggc tgctcggaag tctccggcct
    10681 tgccgaacgt gcgacgcggg atcagttgga gagctcaacc cccaaaccga gcgagaacaa
    10741 agcctgaacc cgaggcagtc gtagcacctc tcgatcgtct acatagaact ggccactcca
    10801 gcgcgccagc ggaagttcga gcgcgccgtg tgcgaaagcc aagagccgac cacccggttt
    10861 gtagatcgcg ccggcttccg cccccagcga ccacatcgcg cggtactttg cggaactctc
    10921 acgaaaaact cctgacgctc gctccacgcc caagcccaaa gtgggccccg caaacaatcg
    10981 gaagcctgcg ctgtcgccaa cgagcccaag cgatccgccc gccactaact cgcgggtgct
    11041 cacatagcgg tcttcggaga gcgactcgaa ccgtcgcgcc agcggggttc tcaagctcgc
    11101 gctccagtag ctccccggaa aagccgcgaa caggcccttc agcgtgagat gggcgtccgc
    11161 tcccgacgag ctgacaccca aacttgcacc gagcatggcc ctaactgggg ccgcagtacg
    11221 cgggctggtc tgcgaaccca attgtaccgt gctggagtca cgctgtaccg ggtcgaccgg
    11281 ttccctctct cttggctccg ggcttctcga attcgtctct ggaacgccct ccgcaagcgg
    11341 cgcgaggcct ccctcctccc cggacaacgc gcggaagtag cggtccagca ccagcgcgat
    11401 tacctccgcc agggtaacgc aactttcgtt ggccgtcggc agctcacgac gtagcttcag
    11461 ctggccttgc ccgtcgaaga gctcgagctg aacgaagtca cgaggttccc cctctggccg
    11521 atgtccgagc gtgtaacggg ccttccacgc tggaccggag gggaactcga accgcccgag
    11581 gcgcctctcc actgccgcac gagcccgggt caagtcgggg caaagagcat caggtgagcc
    11641 ggattcgagc gtcaagccgg cgttctcctc gccgaaggcc atgcggctac aaaacaagca
    11701 agccgacagc acgaatctcg tactccgact cgacgcggat cggacagcga cgcctctcac
    11761 gttgtcccgt ttaatgggtg attgcctgat tctgtcaacc agcgactgcc cagcactcga
    11821 ccaagcagcg cacgctagcc cgaagccgta cgcctgcaag ctccagcacg gacaaagaaa
    11881 ggacgcttcg gcgacgctcc gccagtgctg cccgtccgaa tgccatggat ccgagcattg
    11941 ccgtcaaagg agctcttgcg gacccgcgcc ttgtatcgaa tacacgaaac taggtttggg
    12001 gacgcgtagc cagcgctccg taatggtatc tttgagagtt ttgaaagcgt ccttgccgtc
    12061 cacgtagcgc gcgaccaacg tcaaggcgcg cgcgcgatcc cctcgtgctt tgatttgtag
    12121 aacgaggcga gcaagctcac gcgtggccgt ttctgcacgt gcgaagtcga cgtcgaagca
    12181 acccatgtct tctccattgc gcgcgagctc ggtcgggcgc caaatcacca gcttggcgtc
    12241 ctgcagcacg cccaattgaa tggccgcgag ctgactgtag ttcttcggcc gacccgcagc
    12301 gtcgtacatg ccctgcgcga tatgcccgaa ggcccacgca atctcgcgaa gcagagcctg
    12361 ctgcacgagc tcgctgggta aagctccgcg cgatacgagc caatgcggca ggtacaaggc
    12421 cgcgctttgc gccttcaact cctccaaggt cgccgcaagc gctcccccga aagccgcgtg
    12481 atctacgcgg cccgcgactt tgtattcgtg cgcggggccc aggttgtgag cggcctcgtg
    12541 cagcactaca cccaacacgg cgggctcttc gctcgaagtg acccgcgcgt aactcgcctt
    12601 gcagaacaac gacgcggcct ggcgctcgaa actccgacgg ctatcccagt ccgtatacag
    12661 attggccatc atcaccgtgc gcccgccgcg ttcggcgacc gggccccagt tcggcaacga
    12721 ctgcccgacg gtcgaaccgt gaggatgtcg gctatccccg gcgttgagca cgatgtcgat
    12781 gaagtcaggc aacttgaagc caacctggcg cgcaacgtaa ggcttgccgg ccaagctcgc
    12841 gagctcggtt tcgagctctt gcttcagtgg atccaacttg tcccgccact gcttggaggt
    12901 gtcattgatg cgcgcaaacg tcagcgcaaa gcccgccttc caggcgcacg gctcgtagta
    12961 cacttcgtcc ggcgcgacgc gcaggtagta acgcgagttg tcgctgccca tggccaccca
    13021 agcctcgttg gcgggctccc actggttggt gcggaatgcg gcggacgcgg ccgacaagta
    13081 gcgtcgtaac gctggctcct cctgtccaag cgcggcctcg gcctgctcga gctcgcgcgc
    13141 gacggcttcc atgtcttctc gataggcgac atgatacgga gtcgcgcgaa atccagtgcc
    13201 tgcggcctcc acgacgctga agtgatcctt gagcgcgtcg gcgttgggct gctgctcgag
    13261 cagacgacac caacccggca agcttgcgtt ggcgatcgct tcgttcgtct ggatgttgct
    13321 cggataaagt ccgaagctcg gcttgttgcc actcgggagc gcgttgcagc tcgctagctt
    13381 ctccgtcttc ggggccacgc aggtcgggcc ctgattgcga aagtacagag ctgcgctctc
    13441 cggatcatcg gcagctagcc ctgcctccaa gcccagcgtc ccacgctgcc gcgcatacaa
    13501 gcgctcgatg cctcgcgcgg cgttgagaac gtgggccacg agcgctcgct ctcccgccga
    13561 agccttggaa aaatccgtgt cgaccagcgt tggacgacct tgcaagagtt ccaggcgcac
    13621 gctttctcgg cgctgcacct cggcgggcgc agcgttcggc gggatcggtg ggtcagtctg
    13681 aagacgctcg taaatgtctg cgaaccgtgg agtgaactga gccgcaggat tgacgaactc
    13741 cgcgcgcgcg tgctgctccg caccgaacag gatggccagc tcttccggat ccaacgcgcc
    13801 caaagccccc gcatccttgc gccaaaacac gggcacattc caaagcacgg cacgctgatt
    13861 gaactcgtcc cggtcgagtc gattgaagtg ctccgacgcg gggaccccgt tggaagtctg
    13921 gccctgcaat ggggggccgg ctttgggttc cggtcccttg ggtgtgggtt tcaaaacgct
    13981 gggtgcactg cacgcggcca gcatcgccac gcaccaagct accgtgctct gcgagcgcgg
    14041 gatccacgag cgctgtatcc gcggttcatg cacacaatac ctaacgcgcg cggtctgatg
    14101 cttcggagca gcagggggtc gcttcgccat cttgcgcatg ttcacttgtt cggcggtcga
    14161 gcatagaacg caagctcttc ctcatgaatg cttgtcattc cgatcagaat cctccatgaa
    14221 gaggccaggg ccacgagacg aatgcggttc gggcgatgcg ttacccggtt gtttggacag
    14281 tcgctcggcg ggttaacgct gagttggctg ggttggctcg cgggtccact cgaggctgcg
    14341 cacgccgggc aagcttccgc atcagcggca gcaatggacg tggatcacgc ggctgcggac
    14401 gcagccaaac acgaatccgc gcgcgacgga gcgacggcgt caccccggat tgagctaaga
    14461 gtgccgcaag attgcggctc tgaagcggag tttcgccgag cactgacgca acgtttgtca
    14521 gccgcggatg ttcgcgatct gccagacacg cacgtgcgaa tcgagccctc caactccggg
    14581 ttcgagcttt ggctggaatc cggagcggaa agacgcacgc tgcgagaccc tcggtgccga
    14641 gatctgttcc gagcggcggt ggtgatggta gtcgctcacg ccgagcggag acatgcacca
    14701 gcgcccgcgc cagtttcaag gccaagcgag gagatgaaag gcccggcagc tacggagctc
    14761 gagccgcgag gccccggtgt aatgctcgca gtcggcccga ccttcgggct tcttccgcag
    14821 ccggcgtggg gcgtcagcct cgaaggaagt tggcgaacag ggcgttgggg aggagcactc
    14881 tcgctgcgtt atctcgcccc ggtggagaag gaatatcgcg ctcccgtgga gctccatgcc
    14941 caagcattcg gagcccatct tggagcgtgg ttcgaacctt ggcctgagct tcggttgcac
    15001 gccgggggcg ttacatatgc cgtcttgggt cgaggggtcg gcagtcaggt agagggttcg
    15061 ggccgagttt gggccgctgg gcccgtgctc ggagcgctcg ccgtgccggc gcgcacgagg
    15121 catctctggg taggagtggg tgccgaactt catgtggaag tcgtacgtcc aagcttcgaa
    15181 atagtcccgt acgaacagat ttttcgaagc tcactcgccg tgggtagcgt tttcttggct
    15241 attgctccgg tgtttcgatg acggattcgc catgctgcga ggactaacgc gagcatggcc
    15301 accggaactc tcgacagccg agccctcacg ccccaggcgg cgccgggtga gcacgctgtt
    15361 cgggcgcccg gaatccaaga ggtctatgca gaacacttcc gatacgtctg gcgctgtcta
    15421 cgaagtctag gagttcgcga cgcggttctg gacgacgcgc tgcaagacgt ctttctcgtc
    15481 gtgcaacgca agcttgcgag cttcgagggc aagtcggagc ttcgaacttg gctgtatgca
    15541 atcgcgttgc gggtggcgcg gcgctaccga acccgtccca aagaagagcc gggccccgaa
    15601 gcagacgggg aacacgcggc aatcgacgcc gaggcttgcg ccaacttgga ggccagcgtc
    15661 gagtgcaacc agcgtttgga gttggcgcga cgggcgttgg acgctctgca cgatcacaaa
    15721 cgtgaggtgt tcgtgctcgc ccaaatcgag caaatgtcgg ccccggagat cgccgagatc
    15781 acgggcctcc ctctcaacac ggtgtactcg cgtctgcgcg cagctcggct ggctttcagc
    15841 gctgaagtac gccgcctcga agagcttccg aagaggttgc catgaaaccc gaatcctcga
    15901 tcgatgagct ggtcgatgcg ctgcgcgccg atctgccgag cccgcgagac gaggcccgcg
    15961 tgcgcgcgcg tctcgccgcg gtgggcgtga gcgtaggcgc gggtgtgacc gcgggcggcg
    16021 ccgcggccgc gccgatcacg aaggccttat tgccgggagc caagctggcc gcgaagttgt
    16081 acgccctacc gctcacgatc aaggcgtctg tggtcggagt cgcgtcggtc tcggcggtgg
    16141 tcactccgct gtggctggcc tcgcaggccc gggagatgca ccgcgcagcg ccaagtacgg
    16201 tttcctcgag tagtgcgttg tcgaaggccg cggtcacacg acaagcgaat ggtgatgtgt
    16261 tggcgtcgaa gcccacgccc gctccgcccg cgactcaacc tcccaccgat tctccgccag
    16321 accgttcgcc gaagctcacc cagagctcgc cggagccgaa gcgtccgctc ggcgcgccac
    16381 gagcaccctc ggttgctctc gagccccggt cgcgttcggg actgcgtagt gcgaacgaag
    16441 agcctgcggc gctattgcct cgcagccccg gcaactccgc cgcgacgcac gctgtgatcg
    16501 ccgtgtcccg gagcagtgtg ccagtgccca cgacgcgtcc cgacgtcacc gcgcccagct
    16561 cgatcacggc tgaaccgacg caggcgatcg gcaccgcgac gttcgcgcaa gtcgagcccg
    16621 cggcggcgcc aagccccgtt caagctccaa agctcacgac cgcgagcggc tccgagagtt
    16681 cactgcgtga agagaccgcg ctggtagaca cggcgctgat cgccctacgc gatggtgacc
    16741 gcagcaaggc gcgacttttg ctcgagacac atgcgcgacg ctttccccac ggtcttttgg
    16801 cgcgtgaacg ggaacgcgcg cgacgcaagc tgctgcaagc aagcggcagc gactcgaccc
    16861 attgatccga ttcgcgactg aatacaaatc tcaacgagct taaagcaact gcgcagctcg
    16921 aggctgcgca cgcataaccc acatagattg gaggaacgat ggcaattcta ggtgagaaag
    16981 tggctcgcga cggccactcg cgcgctcagg ccccgagcaa cacaaccggc aaacgccgtt
    17041 atcgctggct gtgggccgtg ctgttgctcg
//
LOCUS       DASTKU010000894         5432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_621077, whole genome shotgun sequence.
ACCESSION   DASTKU010000894 DASTKU010000000
VERSION     DASTKU010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621077"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(477..707)
                     /locus_tag="VFQ61_31435"
     CDS             complement(477..707)
                     /locus_tag="VFQ61_31435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959061.1"
                     /translation="MDRASIIGGLLIAFAFAFQVWVTVRVFRSKLYEGSQKSAQAKLI
                     WLLPVLGAVIAFSVLTSEEQADRRVNNRELRG"
     gene            complement(996..2711)
                     /locus_tag="VFQ61_31440"
     CDS             complement(996..2711)
                     /locus_tag="VFQ61_31440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959062.1"
                     /translation="MQSTSGRPSFGQGAKPSALSARTVARTPVTTRKRASFGFACALG
                     LFGCALLAIACASRNEDLFRNDGGGSPNSGTESRGSGGDVATGSGGNSDSSGGMGSVS
                     CTTGMACGAACVDTLTSNEHCGDCNTRCPAATTCQAGVCTCANGQASCNDVCTDTSSS
                     EEHCGKCNQACPPRAECAGGACVCPGQETVCADKCVDTLTSTEHCGKCNHACPPRATC
                     EAGECSCPGDEAICADKCVDTLTSTEHCGKCNEACPPRATCERGQCACPDQEVVCGDK
                     CVDTQTSTEHCGKCGAGCEGGTCTAGACKCPPGQVNCTGKCVDLETSFGNCGECGHSC
                     TNNQVCEDGKCGCASNAEDCSGTCADLQTDRMHCGDCETACSGNKSCLGGDCRCAIGE
                     IDCSGTCANLLSDPLHCGTCTGACTGNQACLNGRCGCPGTQATCGGNCYDLKNDAKHC
                     GKCETACSPGFVCSQGTCLRSPCDGLCENPESIGPTEDGFRMEPLGEEAHCFEVKGYT
                     PPDMNPRIVCWEFQLGRRLRVNGIDVLCMKDDGVKLTEPRAGGYCVQVSAGGTSGAGL
                     LLPTR"
     gene            complement(2915..3535)
                     /locus_tag="VFQ61_31445"
     CDS             complement(2915..3535)
                     /locus_tag="VFQ61_31445"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HET9959063.1"
                     /translation="MQSLRVLLAPGAGAPSSSPWMRQLSARLEAVGPVTAFDYPYQLR
                     GKRMPDPLPRLIEAHRKAFEEARAQAAHAWLFAGKSMGSRVGCHLSVELEGSEPRALV
                     CFGYPLIGQNGARRDEILLQLRRPILFVQGTRDPLCPLGDLEQVRARLDVPNELHVVE
                     GGDHSLDCLKRDLLSRGITQEVVNAQIDSAVRSFVQRYVLTSVGTA"
     gene            3565..4884
                     /locus_tag="VFQ61_31450"
     CDS             3565..4884
                     /locus_tag="VFQ61_31450"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET9959064.1"
                     /translation="MDNITHGLVGLIVAQLTVQLVDESVPAPVDGPRHATPAESRARF
                     ASWAAWVSVLSNNLPDFDVLYAPVTGGKLAYLLHHRGHTHTLPFALLGGVLIAAVSWF
                     IVRWRRVPLDLSRWRALLALALLGPFLHIFLDFGNDYGVHPFWPLDTRWFYGGAVFIV
                     EPWIWVFTVPPLFVVTRSRWGRAVLALILAAGIGLAFAVSFVGWGPRLALCVGALAFS
                     GFCWWQRHRAQRLTRVGTGGLLVLLGSCWMNAYLAQQHMFAAARRLGFEPHQVLAPVL
                     TPSPGNPLCFSAILVTRRGTDYELRTAAVSSWPSLLPAASCRLEPRGFTLPLLPAPAP
                     ERAAMDASVSLQQRWGAPLAQLRALGHQNCEVQAFLRFARAPFWLPSGPDAWVIGDLR
                     YDRRPDLEFAELRVPRAPEHCPDWIPPWTPPIHELFSRGAGVHRTTP"
     gene            complement(4906..>5432)
                     /locus_tag="VFQ61_31455"
     CDS             complement(4906..>5432)
                     /locus_tag="VFQ61_31455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959065.1"
                     /translation="LACDLTRVVTLQYSDSWGVNYSDYMIGSGKEALGTWSDHFISHK
                     LDDTDRATDLDGLDRTEAQRIADARVVLTSRFKVRRFAYLVNALKNVKTPTGTLLDET
                     LAMYCSETGDGDSHSRKDMPILLAGHVGGFETGRSVAATGKPTGALHASIIKRFGFDV
                     SNYGSPAGSPLPEL"
BASE COUNT          880 a         1735 c         1714 g         1103 t
ORIGIN      
        1 gagcagatgt gctgcgggaa ggacccgagc gagcctcgac gtcctcctcg ccagcgagtt
       61 agttactcca ggaggagcac gggtggaagc tcaagcgtgg gacaattccg caactgaggt
      121 ttcagggccg ccttcatttc gactgcccag atcggaaaac ccgcaagggg gatgggtttc
      181 ttgccccgtg acgttcgcgc cgtggacttg ggcaagccag ccacggcggg acacggttag
      241 attgcggcgg gaaagctacc ggagaggcgc aggcgggatt cgctgattcg aagttcgcgt
      301 tgactcagcg gttcatctcc ctgctcttcg actctcttct ctcttggttc tttcctcttg
      361 gttcttctct cttggttgtt cttgccggtt gtttctatat ctcttctgcc gccccggttt
      421 ggccggttgc gggcccggcc ctcgccggta gtcgagggtt ggtgaaatat ctgaattcaa
      481 ccgcgcagtt cgcggttgtt cacacggcga tccgcctgct cttcggaggt gagaaccgaa
      541 aacgcgatga ccgcgccgag cacgggcaga agccaaatca gcttcgcttg agcagacttt
      601 tgggaaccct cgtagagctt ggagcggaag actcgtacgg tcacccacac ctgaaacgca
      661 aaagcaaacg caatgagaag ccccccaatg atactggcgc ggtccatgat ggccggaagt
      721 ttggtctgga gggctgccgg cgcaaggcca atagacccag ccctgcccat tgggtcagga
      781 ggacggggcc attcgggcag gggagcagcg ccccggtaga agagatgcct gcacgtagga
      841 ataggtagga gttctaccct tactagccgg gtaagccgct ctgctcctcc gcccgcgccc
      901 cgcttcttag agcccatcac aacaggagcg agtcgccggc agccgatagc ccgctctcgg
      961 caaccgtctc cgccgctcct attgtggatg aactctcagc gcgtcggaag gagtagaccg
     1021 gcgccgctgg tgccgcccgc gctgacttgc acgcaatagc cgcccgcgcg cggctcggtg
     1081 agcttcacgc cgtcgtcttt catgcaaagc acgtcaatcc cgttgacgcg caagcggcgc
     1141 cccaactgaa actcccagca cacaatccgg gggttcatgt ccggcggggt atagcccttc
     1201 acctcgaagc aatgtgcctc ctcgccgagc ggctccatcc ggaagccgtc ctcggtgggc
     1261 ccgatgcttt ccgggttctc gcacaagccg tcgcacgggg aacgcaggca agttccctga
     1321 gagcagacaa agcctggcga gcaggcggtc tcgcacttgc cgcagtgctt cgcgtcgttc
     1381 ttcaggtcgt agcagttgcc gccgcaggta gcctgagttc cggggcagcc gcagcgcccg
     1441 ttcaagcacg cttggttgcc ggtgcaggcg ccggtgcacg ttccgcagtg caagggatcc
     1501 gagagcaagt tcgcgcaggt gccgctgcag tcaatctccc cgatcgcaca gcggcagtcg
     1561 ccgcccaggc agctcttgtt gccactgcac gcggtctcgc aatcgccgca gtgcatgcga
     1621 tccgtctgca gatcggcgca ggtgcccgag caatcctcgg cgttgctggc gcagccacat
     1681 ttgccgtctt cgcacacttg attgttggtg caggagtgcc cgcactcgcc gcagttcccg
     1741 aagctggtct ccaggtctac gcacttgccg gtgcagttca cctgtcctgg ggggcacttg
     1801 catgcgcccg ccgtgcaggt gccgccctcg caaccggcgc cgcacttccc gcaatgctcg
     1861 gtgctggtct gggtgtcgac gcacttgtcc ccgcacacga cctcctgatc cgggcaggcg
     1921 cattgcccgc gctcacaggt tgctcggggc gggcacgctt cgttgcactt gccgcaatgc
     1981 tcggtgctgg tcagcgtgtc gacgcacttg tctgcacaaa tcgcctcatc accagggcag
     2041 ctgcactcgc ccgcttcaca ggttgcgcgc ggcggacacg cgtgattgca cttgccgcag
     2101 tgctcggtgc tggtcagcgt gtcgacgcac ttgtcggcgc aaaccgtctc ctgccccggg
     2161 cacacgcatg caccgcctgc gcactccgca cgcggcggac acgcttgatt gcatttgccg
     2221 cagtgctcct cgctcgagct cgtgtccgtg caaacgtcgt tgcaggaggc ttgaccgttg
     2281 gcgcacgtgc aaactcctgc ttggcaggtc gttgcggccg ggcagcgcgt gttgcagtcc
     2341 ccgcagtgtt cgttgctggt cagcgtatcg acgcaagcgg ctccgcaggc cataccggtc
     2401 gtgcagctca cgctgcccat accgccgctc gagtcggagt tacctccgct cccggtcgcc
     2461 acgtctccac cgctgcctcg gctttccgtt ccggagttcg gcgagccgcc gccgtcattc
     2521 cgaaacagat cctcgtttct cgaggcacag gcgatggcca acagggcaca gccgaacaga
     2581 ccgagagcgc aggcaaaccc aaagctcgct cgtttgcggg tggttactgg cgtccgcgcc
     2641 acagttcgcg cggacagagc cgacggcttg gcaccttggc cgaagctggg gcgacccgaa
     2701 gtactttgca tttgagttgc gtctccatcg acccggccgg aggacggggt agaaatgcct
     2761 tagcgcttgg gagccaaacg atcacgtgct ctctgcgagt ttcatgatcc ggggcggtcc
     2821 cgccgatcgg ctacatctcg gcccattgcg ggcgcaatgc agtcaccctc tcgtattgtt
     2881 gtcaaaccat tttagccgcg cttgggtggg gtgatcacgc ggtgccgact gatgtcagca
     2941 cgtagcgctg aacgaatgag cggacggctg agtcgatctg cgcgttcacc acctcttgcg
     3001 tgatgcctcg tgacagcagg tcccgtttga gacagtcgag cgagtgatct ccaccttcga
     3061 cgacgtgcag ttcgttcgga acgtcgagtc tcgcgcgtac ctgctccaga tctcctagcg
     3121 ggcaaagcgg gtccctggtg ccttgcacga acaaaatcgg gcgtcgcagc tgcagcaaga
     3181 tttcgtctcg gcgcgcgcca ttctggccaa tcagcgggta accaaaacaa accaaggctc
     3241 gaggctctga gccctcgagc tccacgctca agtgacaccc gacgcggctc cccatactct
     3301 tgcccgcgaa cagccaggcg tgcgcggcct gtgctcgggc ctcttcgaac gctttgcgat
     3361 gagcttcgat cagcctcggt aatgggtccg gcatccgctt gccgcggagt tggtaagggt
     3421 agtcgaacgc ggtgaccggg ccaaccgcct cgagtcgcgc ggagagctgc cgcatccacg
     3481 gcgaactgga cggcgcgccc gcgccggggg cgagcaaaac gcggagtgat tgcatacgtg
     3541 tcacgaagct gatgaaagaa ccaggtggac aatatcacgc atgggctagt cggtctgatc
     3601 gtcgcgcagc tcacggtcca actcgtggac gagagtgtgc cggctccggt cgatggaccg
     3661 cgccatgcca ccccggccga gagtcgggcg cgctttgcgt cgtgggcggc ttgggtcagc
     3721 gtactgtcca acaacttgcc ggacttcgac gtcttgtacg cgccggtcac gggcggcaag
     3781 ctcgcatatc tgcttcatca ccgcggtcac acgcacacct tacccttcgc gctgctcgga
     3841 ggagtgctaa tcgcggctgt cagctggttc atcgtgcgtt ggcggcgtgt accgctcgat
     3901 ctatctcggt ggcgggcgct actggctttg gccttgctgg ggccgtttct gcacatcttc
     3961 ctcgacttcg gcaacgacta cggagtgcac cccttttggc cgctggacac gcgctggttc
     4021 tacgggggcg cggtgttcat cgtagagcct tggatttggg tgttcaccgt gccaccgctg
     4081 ttcgtcgtta cgcgcagtcg ctggggccgt gcggtcttgg cgttgattct ggcggccggc
     4141 attggcttgg cctttgcggt atctttcgtc ggttggggcc cgaggctcgc gctttgcgtc
     4201 ggtgcgctgg cattctcggg attttgttgg tggcagcgcc atcgagccca gcggctcact
     4261 cgggtgggta cgggcggatt gttggtccta ctcggtagct gttggatgaa tgcctatctg
     4321 gcccaacaac acatgttcgc ggcagcgcgg cggctaggct tcgagcccca tcaagttttg
     4381 gcgcccgtac tcacgccttc tccgggcaac cctctctgct tcagtgccat tctggtgacc
     4441 cggcgaggaa ctgactacga gctccggacg gcggcggtct cgagctggcc tagtctgttg
     4501 ccggctgcga gttgccggct cgagccgcgc ggcttcactc tgcctttgtt gcctgcgccc
     4561 gcccccgaac gcgccgcaat ggacgcatcc gtctctttgc agcaacgttg gggggcgccg
     4621 ttggcccagc tgcgtgcgct cggacaccag aactgcgaag tacaggcgtt cctgcgtttt
     4681 gcacgggcgc cgttttggct gccgagcggg cccgatgcgt gggtgatcgg cgatttgcgc
     4741 tacgaccggc gtcccgatct cgagttcgcg gagctgcgcg tgccgcgagc cccggagcat
     4801 tgtccagatt ggatcccgcc gtggactccg cctattcacg agctcttctc gcggggggcg
     4861 ggtgtgcacc gcaccacgcc gtaggcgcgc gtgaaaagca ccacgctaca gctcggggag
     4921 cggactacca gcgggtgaac cgtaattgct gacgtcgaat ccaaatcgct tgatgatgga
     4981 ggcgtgaagc gcgcccgtgg gtttacccgt cgccgcgacg ctgcgtccgg tttcgaagcc
     5041 accgacgtgg ccggcgagca agatcggcat gtctttgcga gagtggctgt ccccatcccc
     5101 agtctccgag caatacatgg ccaaagtttc gtccagcagt gttccggtcg gtgttttcac
     5161 gttcttgagc gcgttcacga ggtacgcgaa acgccgaacc ttgaagcgtg acgtgagcac
     5221 gacccgggcg tcggcgatgc gctgggcttc cgtgcgatcc aagccgtcga gatccgtggc
     5281 gcgatccgtg tcatcgagtt tgtgagatat gaagtgatcg ctccaggtgc caagcgcttc
     5341 cttgccgctg ccgatcatgt aatccgagta gttcacgccc cagctgtcgc tatactgcag
     5401 cgtcaccacg cgggtgagat cgcaggctaa cg
//
LOCUS       DASTKU010000895         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_621195, whole genome shotgun sequence.
ACCESSION   DASTKU010000895 DASTKU010000000
VERSION     DASTKU010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621195"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            992..1213
                     /locus_tag="VFQ61_31460"
     CDS             992..1213
                     /locus_tag="VFQ61_31460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959066.1"
                     /translation="MPRQILETNWLELPCEPDLVVRRLGENAVAFMALRIASSELAED
                     STDWACAQRSEGSLQLELTGNSFWLRGAT"
     gene            1443..>3096
                     /locus_tag="VFQ61_31465"
     CDS             1443..>3096
                     /locus_tag="VFQ61_31465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9959067.1"
                     /translation="MSTKLELERPFALALVEEFPRLSIWREQLKIGRRIDVSFDALRP
                     EELAFLEQAYARAGGEYRNRAANVAALLAAVQSGEPRFGEHDDLERLLPALALYLSKD
                     ALRGWLFNANVAGKALAYVVYRLDYVPASSEEVGKILLELRAYGRGMMMTKSVRICSS
                     DIVNKSVPEILASKGLLKETPALIAAYDAAAQRYFDWQSRYGSQFLAAGVGFYAEDPT
                     SPRRESDWSRKDLIVLSTSGGPARVVNDEGILPAQAVAADATGDILGPYLRKAGKSDR
                     YSSEDEVQASRAQIPAGLFTQIPVHGYLFVFHLELHHHLWVHVDDLAAYEYSPSLKQK
                     LVLPPEQTDLIDILTAEMDVLMDDIVVGKSGGTTVLCSGPAGVGKTLTAEVYSEIVGR
                     PLYRVHSGQLGLNVAAMEQALKEALTRAQRWGAVMLIDEADVYIKRREDDMTMNAVVG
                     VFLRVLEYFNGLLFLTTNRVDDIDEAIVSRCIALLRYQPPDRAGRQKIWQVMNEQFEL
                     KLSSALIEELAELFPEASGRDIKGLAKLVAKYCRQKKVGPTLE"
BASE COUNT          573 a          883 c         1004 g          636 t
ORIGIN      
        1 cgagcaaagg cccggagtgc atctacagag tctggctcaa gtgtttgccc gcgtagctga
       61 gttcgctcgg gctgctccgg gtgagcgaac gaggtcgtat gtctcctatg gctgcgcagc
      121 gtcgctctga ttcgggcggg ccgctctgat tcgggcctgt tgtttcgcac gagtgccggc
      181 gaactcgcca cgtgttgtct gggaacgcgc tgaccttagc ccatttgccg cggcgtggtc
      241 agcttgctgt gcctcgtgtg cttcagtcat ggatatcgtt ccagcgatcc gcaatgggcc
      301 gccgatgttt tatgcacgat acaaaactcg ttcccaggtc gcggcacctc cgcatagcta
      361 gctcgagttg cgctggaggg cgcggaagcc cagagtgcca gccgcgctaa tggcagccgc
      421 tgggggacat tgaggcaggt ctaaagttgc tcaatggttt cgatacggtg ggattcaggg
      481 acgacactct ctcggcagct tcgagactcc cgcttccatg gctccgcggg ggaatagatg
      541 gcgtatagag cttgctcagc tctcgccggc tcacgcgcaa gattggcgcg agcatgacac
      601 gcatgccgca ttctcggagc aacctcaagg aaacagctcg gagtttcgcg gctgcggtac
      661 atgccgcggg cgttgttcat gccgagggcg gcatgccgtt cgttgttcat ggtggctgcc
      721 caaggattgg tcgtggcaac gattctggtc gccgagccag tgcggcagct gggtagtagt
      781 ggctaccttc catcctgcag gttctgagta accgttcggc tgcattgacc gtcaaccgag
      841 accgtccagc ggtagttcgt aaagacgtca ttgccaattg cagtaaagcg tcgaattcag
      901 cgccaacacg agccgtcaat caccgagaca tggatcgtgt ggtactgaga acgatttggc
      961 tgagctgcga tacgggcaac gatgaagtgg gttgccacgc cagatcctcg agaccaattg
     1021 gctcgagctt ccctgcgagc ccgacttggt ggtgcgcagg ttgggagaga acgctgttgc
     1081 gttcatggct ctgcggattg catcctcgga gctcgccgag gacagcaccg attgggcgtg
     1141 cgcgcagcgc agcgagggct cgctgcagct ggagctgact ggaaacagct tctggttgcg
     1201 cggagcaacg tagactttgc gacacttctg tcgctcctcg aaccagggct cgctggcact
     1261 gctaccgagc tctgttgccg attgccgtta cccatcgctg cacgcgggct cgaggcaaag
     1321 ttcgtgaagt cgcgcgggct tggctcgcgg tttgcttcga tcgagcgaat gcctaccgct
     1381 accatcctcc gtttcggctt cagcagagtg gcgattgatt gcggaagtct tgaggtactc
     1441 acgtgagcac caaactcgag ctcgagcggc cctttgcgct agctttggtc gaggagtttc
     1501 cgcgcttgag catttggcgc gaacagctca aaatcggtcg tcggatcgac gtctcgttcg
     1561 acgcgctgag gccggaggag ctggcgttct tggagcaggc gtatgcgagg gcaggcggtg
     1621 agtaccggaa tcgcgccgcc aacgtcgcgg cgctcctcgc ggcggttcaa tctggcgaac
     1681 cgcgctttgg cgagcacgac gatttagagc gattgttgcc cgcgctcgcg ctctacctgt
     1741 cgaaagatgc gcttcgcggc tggctattca acgccaacgt cgcgggcaag gcgctggcct
     1801 atgtcgtgta tcggttggac tacgtgccgg cttcgtccga agaagtgggc aagatcctgc
     1861 tcgagctacg cgcttacggc cgggggatga tgatgaccaa gagcgtgcgc atttgtagca
     1921 gcgacatcgt gaacaagagc gtacccgaga tcctcgccag caagggcctg ctcaaggaaa
     1981 ccccggccct gatcgccgcc tacgacgccg cggcacagcg atacttcgat tggcaatccc
     2041 gttacggcag tcagttcctc gcggcggggg ttggcttcta tgccgaggac ccgacttcac
     2101 cgcgacgtga gagcgattgg tcgcgcaagg acctgatcgt attgtctacg agcggcgggc
     2161 ccgcgcgcgt ggtcaatgac gagggcattt tgccggcgca ggccgtggcc gccgatgcga
     2221 cgggcgacat tctcggaccg tacttgcgga aagcgggcaa gagcgatcgc tacagctccg
     2281 aagacgaagt tcaagcatct agagctcaga tcccggcggg gctgttcacg caaatccccg
     2341 tgcatggcta cctgttcgtc ttccacctgg agctgcatca ccacctttgg gtccacgtgg
     2401 atgatctggc cgcgtacgag tactcgcctt cgctcaagca gaagctggtg ctacctccag
     2461 agcagacgga tttgatcgat atcctgacgg cggagatgga cgtgctgatg gacgacatcg
     2521 tggtcgggaa atccggcggc accaccgtgc tgtgtagcgg tcccgcgggc gtagggaaga
     2581 ctctgaccgc ggaggtgtac tccgaaatcg tgggtcgtcc gctgtatcgc gtgcattcgg
     2641 ggcagttggg gctcaacgtg gccgccatgg agcaggcgct caaagaggcg ctcacgcgag
     2701 ctcagcgctg gggtgcggtc atgttgatcg acgaagcgga cgtttacatc aaacgccgtg
     2761 aagacgacat gacgatgaat gcggtggtcg gcgtcttctt gcgcgtgctc gagtacttca
     2821 acggcctctt gttcttgacg accaaccgcg tggacgacat cgacgaagcc atcgtctctc
     2881 gatgtatcgc gttgttgcgc taccaacccc cagaccgcgc gggtcgccaa aagatctggc
     2941 aggtgatgaa cgagcaattc gagctgaaac tgagctctgc gctgatcgag gagctagcgg
     3001 agctcttccc cgaggcgagc ggtcgggaca tcaagggcct tgccaagcta gtggccaagt
     3061 actgccgcca gaaaaaggtt ggccccacgt tggagg
//
LOCUS       DASTKU010000896         8930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1293009, whole genome shotgun sequence.
ACCESSION   DASTKU010000896 DASTKU010000000
VERSION     DASTKU010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8930
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293009"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2351)
                     /locus_tag="VFQ61_31470"
     CDS             complement(<1..2351)
                     /locus_tag="VFQ61_31470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9959068.1"
                     /translation="MTPHTGRTLKSKLLQMLVASMSFTGLVTLASFSWSNYQLEKARL
                     SEIEQQIYKSVSSKASTLATSHALALKGLAADNAISDIRRIVATAVQDDTDLVYGVFL
                     SAEDEPWAYASPTHPYRPREGSIARSVLLEIGLRSPRTLEPTPRPVLSGPRADNGTSA
                     ELVQRKVRAFGQDIEEWAAPVWSDDAERLGTVIYGFSNQRRMAALREAAQRSQQALAR
                     AFLTSFALGLFTMLAGIAWVLRSSSRITKPLATLTGAANQIASGKRGVRVDIRTDDEI
                     QVLAAAFNHMLEANDDAIRRLETTTARALAADRLKSEFLANMSHEIRTPMNGVLGMIR
                     LIQSQPLPGKLARYVSTIDASANALLTIINDILDFSKLEAGKYTLLPIAFDVRLLLQE
                     VIELWASRAQEKRLVLVHRVAPEVPETLIGDPDRLRQVLNNLVGNALKFTDKGEVFVQ
                     IDLASESENRVVLRFAVQDTGIGIAPEQLPKLYQAFSQVDGTLVRQYGGTGLGLAIAK
                     RIVNMMGGELELQSSLGHGSTFSFTVDMKPARGEAVNANRGAPGGRPPNPVTLPPDSL
                     GKRRRVLLMEANTRWREVIEEHLRVWRMLADSCSNAEQGLARLAERGVEYDAVLISDD
                     IPDASVDQLVLRVRALPQAKAVPLILLTEFSATSMPNSVQAELRAQLQKPLRFSELYD
                     CLVGALPTPSMPQEPEETEPFSLISRKRVLVVDDNEVNRFVVVEELERRGYRVDQAED
                     GQQALEWFEHNEYLCILMDCQMPVMDGYEAARRIRQMEQQRGRP"
     gene            2494..2892
                     /locus_tag="VFQ61_31475"
     CDS             2494..2892
                     /locus_tag="VFQ61_31475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9959069.1"
                     /translation="MPTTRVLLIDDDDIAREFLASLLRDGGYEVVELASPIGATRTIL
                     SECIDVVLLDIFMPNMDGDKSAKMLRENTRLAGLGIVLVSSCEASELGEIAARVRADA
                     VVHKGHARQQLIPTVHRVASRVRKTRSAGS"
     gene            3059..4327
                     /locus_tag="VFQ61_31480"
     CDS             3059..4327
                     /locus_tag="VFQ61_31480"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HET9959070.1"
                     /translation="MQDLRPCSARCKYVRVDLPKSHLIASLFGALLTLGTPACSRGRD
                     TARGSGDVSSRAVPSGASAGTDAAHPNAAADAAKLPLDRIQLPKGFRIAAYATGVEDA
                     RSLTLSPTGTLFVGTKSKGRVYAIPNRDRDTVGDRVIEIASDLNSPNGVAFRDGALYV
                     AEIDRVLRFDRIEQQLEGSPKPTVVTDRYPKDEHHGWKYIAFGPDGKLYIPIGAPCNV
                     CDKDADGYSNITRINPDGSGFEIIARGVRNSVGFDFQPGTGQLWFTENGRDMLGDDVP
                     GDELNRLSRPGEDFGFPRCHAGTIQDPEFGKSADCTKYSAPVRVLGAHVAALGMKFYT
                     GKQFPPEYHNQIFVAEHGSWNRSKKSGYRVMLAQLEGERVVAYKEFATGWLRDEIVKG
                     RPVDVAVAPDGALLVSDDEANAIYRISYTG"
     gene            4633..5073
                     /locus_tag="VFQ61_31485"
     CDS             4633..5073
                     /locus_tag="VFQ61_31485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959071.1"
                     /translation="MFTSKGNPKLTSNSLFFGAESFIRQYPQIAQRVVKALVASAKWA
                     ADQESDPAPVFQLWTRSGSTFSSYKEDWTGESLKYKLSPLLDPYVVTRHTFQIEQAKR
                     LGLIRRSFDFGEMVNPTFLSAALKELALVGFWKPRDAQTGKPAS"
     gene            6151..8511
                     /gene="pflB"
                     /locus_tag="VFQ61_31490"
     CDS             6151..8511
                     /gene="pflB"
                     /locus_tag="VFQ61_31490"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET9959072.1"
                     /translation="MPGAVGSELGLGVANAIHGVSPGAHPAPNLRNSKQCPSEPPAAD
                     SEPREWYGFTGQDFRARIDVRAFIRDNATPYEGEADFLAPATQRTRDLWAKLQGAFEL
                     ERARGVLDVSQIPSSITAHAPGYIDKDLELIVGLQTDAALKRAVFPNGGLRMVEASLS
                     AYGYQIDPELKRIFTKYRKTHNDGVFDAYSRDVLRARSSHIVTGLPDAYGRGRIIGDY
                     RRVPLYGVARLIEQKKRERAALDACPATDSVIRDREELAEQARALGELVEMARSYGFD
                     ISRPAASAHEAVQWLYFAYLGAIKEQNGAAMSLGRTSTFLDIYFERDLANGLITETEA
                     QELVDDFVIKLRIVRFLRTPDYNALFSGDPTWVTESIGGMGEDGRTRVTKTSFRFLQT
                     LYNLGPAPEPNLTVLWSPRLPLGFKRFCARVSLDTSSIQYESDELLRCFGGDDTAIAC
                     CVSAMQVGKQMQFFGARVNLAKCLLYAINGGRDELTAEQVMAPFEPIRDDVLEYSTVM
                     QRFDVAMEWLAQTYVSAMNCIHYMHDKYAYERLEMALHDYAPLRTMAFGVAGLSVVAD
                     SLSAIKYAQVRVLRDNKGLAVDYEVNGEFPKFGNNDDRVDQFAREIVESFMAKLRKHA
                     TYRNAVHTQSVLTITSNVVYGKHTGNTPDGRRKGEPFAPGANPLHGRDVLGMVASAFS
                     VSKIPYEHCQDGISLTASVVPNGLGSDVAERESNLVHALDGLFENGLFHINVNVLNRE
                     TLLDAMEHPEKYPELTVRVSGYAVNFVKLTREQQQEVVRRTFHGSS"
     gene            8508..8645
                     /locus_tag="VFQ61_31495"
     CDS             8508..8645
                     /locus_tag="VFQ61_31495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959073.1"
                     /translation="MSNAGAETKTELGTKPAHEEGRFGYVHSEQTGSALDGPGLRTVF
                     W"
     gene            8658..8930
                     /locus_tag="VFQ61_31500"
                     /pseudo
     CDS             8658..8930
                     /locus_tag="VFQ61_31500"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
BASE COUNT         1661 a         2686 c         2882 g         1701 t
ORIGIN      
        1 gggcggccgc gctgctgctc catctgccga atgcgccggg cggcctcgta gccgtccatc
       61 accggcatct ggcagtccat gagaatacac agatactcgt tgtgctcgaa ccactccagc
      121 gcttgctggc cgtcttcggc ctggtccact cgatagccgc ggcgctcgag ctcctccacc
      181 acgacgaagc ggttgacctc gttgtcgtcg accaccaaca cgcgcttgcg cgagatcaac
      241 gagaagggct cggtctcctc cggttcttgc ggcatcgaag gggtaggcaa ggcgcccact
      301 aggcaatcgt agagttcgga gaaccgcagc ggcttttgca gctgcgcgcg cagctccgcc
      361 tggaccgaat tgggcatgct cgtagcgctg aactcggtca gcaggatcaa cgggaccgcc
      421 ttggcttgcg gcagggcgcg cacgcgaagc acgagctgat ccacgctcgc atcggggatg
      481 tcatcgctga tcaggaccgc gtcgtattcg acacctcgct ccgccagccg cgccagcccc
      541 tgctcggcgt tcgagcagct atccgctagc attcgccaga ctcgcaggtg ctcctcgatc
      601 acctctcgcc agcgcgtgtt cgcttccatc agcaacactc gcctgcgctt tccgagcgag
      661 tctggtggca gcgtgacggg gttgggcggc ctgccgccgg gcgcgccccg gttggcgttc
      721 acggcttctc cgcgggcagg cttcatgtcg accgtgaagc tgaaggtgct gccgtggccg
      781 agcgagctct gcagctcgag ctcgccaccc atcatgttga cgatgcgctt cgcgatcgcc
      841 aagccgaggc ccgttccacc gtactgtcga accagggtgc cgtcgacttg ggagaacgcc
      901 tggtacagct tgggcagctg ttcaggtgcg atgccaatgc ccgtgtcctg cacggcgaat
      961 cgcaaaacga cgcggttttc ggactcgctc gccaggtcga tctgcacgaa gacctcgccc
     1021 ttgtcggtga atttgagggc gttcccaacc aggttgttca gaacttgccg caggcgatcc
     1081 ggatctccaa tcaacgtctc gggcacttcc ggagcaaccc gatgcaccaa caccaggcgc
     1141 ttctcttgtg ctcggctggc ccacagttca atcacctcct ggagcagcag tctcacgtcg
     1201 aacgcgattg gcaagagcgt gtacttgcct gcctcgagct tcgaaaagtc gaggatatcg
     1261 ttgatgatgg tgagcagggc gttggcggag gcatcgatgg tgctcacgta gcgcgccagc
     1321 ttgcccggca ggggctgaga ctgaatcagg cggatcatgc ccagtacgcc gttcatgggc
     1381 gtgcgaatct catggctcat gttggccaag aactcgctct tgagacggtc tgccgcgagg
     1441 gcccgtgcgg tcgtggtctc cagtcggcgt atggcgtcgt cgttggcctc gagcatgtga
     1501 ttgaaggccg ccgctagtac ctggatctca tcgtcggtcc tgatgtcgac ccgtaccccg
     1561 cgtttgcctg acgcgatctg gttggcggcg cccgtcaatg tcgctaacgg tttggtgatg
     1621 cgcgacgagg aacggagtac ccaagcgatt ccggccaaca tcgtgaacag gccgagcgca
     1681 aaggacgtga gaaaggcgcg ggccagcgct tgctgcgagc gttgcgcggc ctcgcgcaag
     1741 gccgccattc ggcgctggtt gctgaagccg taaataaccg tgcccaagcg ctcggcatcg
     1801 tcgctccaaa ccggcgctgc ccactcctcg atgtcttggc cgaaggcgcg caccttacgc
     1861 tgtacgagct ccgccgatgt cccgttgtcc gcgcgcggac cggacaatac cgggcgcggc
     1921 gtcggttcga gcgttcgcgg agagcggagg ccgatctcca gcagcacgct tcgggcaatc
     1981 gaaccctctc tggggcggta tggatgggtg ggcgaggcgt aagcccacgg ctcgtcttct
     2041 gcgctcaaga agacgccgta aaccaggtcg gtgtcgtcct ggacggcggt cgccacgatg
     2101 cggcgaatat cgcttattgc attgtcggcc gccaagcctt tgagtgccaa agcgtggctc
     2161 gtagccagag tcgaagcctt gctcgagaca gacttgtaga tctgctgctc gatctcggag
     2221 agccgggcct tttcgagctg gtagttggac cagctgaagg atgcgagggt aacgaggccg
     2281 gtgaacgaca tactcgccac cagcatctga agaagcttcg atttgagggt gcgccctgta
     2341 tgcggtgtca taccgtgtcc ggcatcgcgg cccatgctaa cctgagctcg acgaggcgtt
     2401 agtcaccatg cgggatgtag ggcgcacaca gcgcgcgctg ctggtcgcgc cttatgcagt
     2461 aactctggcg tccgttcttc ccccggtaac ctcgtgccta ccacacgtgt tctcctgatc
     2521 gacgatgacg acattgctcg ggaattcctg gcgtctttgc tgcgcgacgg gggatacgag
     2581 gtagtggagc ttgcttcacc catcggcgca actcgcacca tcttaagcga gtgcatcgat
     2641 gtcgtgcttc tcgacatatt catgcccaac atggatggcg acaagtcggc caagatgttg
     2701 cgagagaata cacgcctcgc gggtctgggc atcgtgttgg tgtcgagttg cgaggcgtcg
     2761 gagctcgggg agattgccgc gcgcgtgaga gcagatgccg tcgttcacaa gggccatgcg
     2821 cgccagcagc tgattccgac cgtgcaccga gtggcttccc gtgtgcgcaa aacccgaagc
     2881 gcgggtagtt gacgaggtat tcccgtgcgg gggaactccg tgctgctctg cgacggagga
     2941 gccaatcacc gtggttgcag aacgcgcgat gagtgcgtga attcacgccc cgcgaaccag
     3001 gcctcacgga aaccacgcct cacaacagcc cgcccgcctg aattgcgaac ataagcgcgt
     3061 gcaggacttg cggccctgta gcgcacgctg caagtacgtg cgggtggacc tgcccaaatc
     3121 ccatctcatt gcctcgttgt tcggcgctct gctgacgttg ggcaccccgg cttgtagtcg
     3181 agggcgcgat accgcgcggg gttcgggtga tgtgtccagt agagccgtcc catccggggc
     3241 gagcgcgggg acggatgccg ctcaccccaa cgcggccgcg gacgccgcca agctaccgtt
     3301 ggaccgcatt cagctcccga aaggctttcg catcgcagcc tacgctacgg gcgtcgaaga
     3361 tgcgcgctca ttgacgctct caccgacggg gacactgttc gtgggtacca agagcaaggg
     3421 tcgcgtatac gcgatcccga accgtgaccg ggataccgtg ggcgatcgcg tgatcgagat
     3481 cgcctccgac ctgaactctc cgaacggtgt ggctttccgc gatggagcct tgtacgtcgc
     3541 cgagatcgac cgggtgctgc gcttcgatcg aatcgagcag caactcgagg ggagccccaa
     3601 accgaccgtg gtcaccgacc gctaccccaa agacgagcac cacggctgga agtacatcgc
     3661 gttcgggccc gatgggaagc tctacattcc gatcggagcc ccttgtaacg tgtgcgacaa
     3721 ggatgcggac ggatactcca atattactcg catcaacccg gatggtagcg gcttcgagat
     3781 catcgcgcgc ggcgtgcgaa atagcgtcgg cttcgacttt cagcccggca cgggccagct
     3841 ctggttcacg gaaaacggtc gagatatgct cggcgacgac gtgcctggtg acgagctcaa
     3901 tcggttgtcg cgccccgggg aggacttcgg gtttcctcgt tgccacgcgg gaactatcca
     3961 agatcccgag tttggaaaat cggcggactg caccaagtac agtgcgccgg tgcgtgtcct
     4021 cggcgcgcac gtagcggctt tagggatgaa gttctacacc gggaagcagt ttccgcccga
     4081 gtaccacaat caaatcttcg ttgcggagca tggctcttgg aaccgcagca agaagtcggg
     4141 ctaccgcgtg atgttagcgc aactcgaagg cgaacgcgtg gtggcgtaca aggaatttgc
     4201 gaccggctgg ctgcgcgacg aaatcgtgaa gggccgcccc gtggacgttg cggttgctcc
     4261 cgacggcgcg ctgctcgtct cggacgacga ggccaacgcg atataccgaa tcagctacac
     4321 gggatagacc ggagaagcgc gcgcgaacgc gcttctccga gcgcttacag tagtgattgc
     4381 cgctcgaaac aaggaagcac tgcgcgcgag cgcggcgcag ctcgcggccg agacgggcca
     4441 gcgcatcatc ccgattcgat ggcctggctg gttgcttgta cgagtcttct aacggtgcgc
     4501 gggttatcgc gaatgaatga attgcggaac acgtaggtac ctgccgtgaa cttcccgaac
     4561 atctcgtagt cagagaagag cttccgcaat ccaccatagg ttgtgctcgg tgagcagggc
     4621 gcggctcgca tcgtgtttac gagcaagggc aaccctaaac tcacctcgaa cagcttgttt
     4681 tttggcgctg agtcgttcat tcgccagtac ccacagatcg cgcagcgggt ggtgaaggcg
     4741 ttggtggcct ccgccaagtg ggccgccgat caggagagtg atccagcgcc agtgtttcag
     4801 ctctggacgc gctcgggatc gacgttctcg agttacaaag aggactggac gggcgagtcc
     4861 ttgaaataca agttgagccc gctgctcgat ccttacgtgg tgacccgaca caccttccag
     4921 atcgagcagg caaaacggct cgggctgatc cggcgaagct tcgattttgg tgagatggtc
     4981 aacccgacct tcttgtcggc ggcgttgaaa gagctcgcct tggtaggttt ttggaagccg
     5041 cgcgacgcac agactggcaa gccggcgagc tgactgcccc ggcccgagaa cggggtcgac
     5101 tgattgaggg cctgcacctc atgggccaat gtccccggaa tccatcgttg cctgcagttc
     5161 gcgtgacatc caggtctggg atacaacgcg atggccggcg cggtgagcgc gggcgcatgg
     5221 gggcaaggag gctgcgccga cgacgtgagc gaacgcacgg cgcgcacgtt caaatgtcgg
     5281 ctcccccaaa tgcgcgcctt gccgagacgc aacgctccac gacgctcgcc ttgctgacct
     5341 cgtcgatgcc gagcgggaag ccgatgtccc cggctcacgc cgctgcaaat cgcgatgttg
     5401 ggtcgaggca gcgaagagct gcttcggccg ttcgtttggt gggggtggtc tcggtacccg
     5461 ccgtgcggaa gcgcccgtgg ccggacacga gctcgaaacg aataccccct ggggtgataa
     5521 acgcgaccaa aagacgaagc gctccggttg cttgcgacgc agccccccgc agcatttgcg
     5581 gcgcagaatc agcggtttgc ggcgagcccg cgctcgtcga tgcagcgctc gattcggcac
     5641 accgcctctc ccgcagcttg ccggacaata gccgtacgct tcagaaatgt ctctcgtctc
     5701 gcgagtactt caggactcgg agctgctgga tctcaggctg cgctcgtgat cagcgactcg
     5761 tttgcacgaa aggggagtgc cggtcgtgaa ttggcgttca tgatgtgaag cgctcgggag
     5821 aacctttcga gctcttcgct gtgggttcga tctcaatctg caagggcgcc tctcattcat
     5881 gccccgactc gctagaaatc gtgagttttt ggcggcgttt cgcgaggtcg gtcggtgtga
     5941 gtgaggccgc gcgccgggaa cagagagctt cttgcgctcc ggtttgggac ggaacgcaag
     6001 gcttgcgtga aaaactgcgg gacgaacggc tcgctgctga cgattgccgg gtttcttggc
     6061 gaaaccaacg aggccgcgtc ggggaggcct ggtccttgca aacgccgcca acaaaagtcg
     6121 aggtagagct tgtcgaacat tctagagact gtgcccggcg cggtcgggtc agagctggga
     6181 ttgggcgtag ccaacgccat ccatggtgtc agtccaggcg cacacccagc acccaatctg
     6241 cgcaattcga aacagtgtcc ttcggagcca cctgctgccg attcggagcc gcgtgaatgg
     6301 tacggcttca cgggtcaaga tttcagggcc cgcatcgacg tgagggcgtt cattcgcgat
     6361 aacgcgacgc cttacgaagg cgaagctgac tttctggcgc ccgctacgca gcggacccgc
     6421 gacttgtggg ccaaactgca gggtgcgttc gagctcgagc gggctcgcgg agtgctggac
     6481 gtctcgcaga ttccgtcgtc gatcacggcg cacgcgccgg gctacatcga caaggatctg
     6541 gagctgattg tgggcctgca aacagatgct gcgctcaagc gcgccgtgtt tccgaacggt
     6601 gggttgcgca tggtggaagc ctcgctgtcg gcctacgggt atcaaatcga tcccgagctg
     6661 aagaggatct tcaccaaata tcgcaagacc cacaatgacg gggtgttcga tgcctactca
     6721 cgcgacgtgc tccgtgcgcg ttcgagtcat atcgtcacgg gtttgcccga cgcatacggc
     6781 cgcggccgca tcatcggaga ctaccggcgc gttccgcttt acggcgtggc ccgcctgatc
     6841 gagcagaaga agcgtgagcg agcggctctg gacgcatgcc ccgctaccga cagcgtgatc
     6901 cgagatcgcg aagagctcgc ggaacaagcc cgcgcgctcg gtgagctcgt agagatggcg
     6961 cggtcctatg gattcgatat cagccggccg gcggccagcg ctcatgaagc ggtgcagtgg
     7021 ctctacttcg cctatctcgg cgctatcaag gagcagaatg gggccgccat gtcgctgggc
     7081 cggacctcta cgtttctgga tatctatttc gagcgtgacc tggcgaacgg gctcattacc
     7141 gagactgaag cgcaggagtt ggtcgatgac ttcgtaatca agctgcgcat cgtgcgcttc
     7201 ctgcggacgc ccgattacaa tgcgctcttc tccggagatc cgacttgggt taccgagtct
     7261 atcggcggaa tgggcgaaga cgggcgcacg cgcgtgacca agacgagctt ccgcttcttg
     7321 cagacgctgt acaacttggg gcccgctccc gagccgaacc tcacggtgct gtggtcccca
     7381 cgcctgccgc tcgggttcaa gcgtttttgc gcgcgggtga gcctggatac gagctcgatt
     7441 caatacgagt cggacgagct gttgcgttgt ttcggaggtg acgatacggc gattgcctgc
     7501 tgcgtctcgg ccatgcaggt cggcaagcag atgcaattct tcggcgcccg cgtgaacctc
     7561 gccaagtgct tgctgtacgc gatcaacggc gggcgcgacg agctgacggc ggagcaggtg
     7621 atggcgccgt tcgagccgat tcgtgacgac gtgctcgagt actcgacggt gatgcaacgc
     7681 ttcgacgtgg cgatggagtg gctggcccag acctacgtca gcgccatgaa ttgcatccac
     7741 tacatgcacg acaagtatgc ctacgagcgg ctcgagatgg cgctgcacga ttacgccccg
     7801 ctgcgcacga tggccttcgg ggtggcgggc ttgtccgtgg tggccgactc gctgtctgcc
     7861 atcaagtacg cacaggtgcg ggtgctgcgt gacaacaagg gtctggcggt cgactacgag
     7921 gtgaacggcg agttccccaa attcggaaac aacgacgatc gcgtcgatca gttcgcgcgc
     7981 gagatcgtcg aatcgttcat ggccaagctg cgcaagcacg cgacttaccg caatgccgtg
     8041 cacacccaga gtgtcctgac gatcacgtcc aatgtggttt acggtaagca cacgggcaat
     8101 acgcccgacg gacgccgcaa gggtgagccc ttcgcgcccg gagcaaatcc gctacacggg
     8161 cgcgacgtac tcgggatggt ggcatcggcg ttttccgtgt cgaagatccc ctacgagcac
     8221 tgccaagacg ggatttcgtt gacggcgagc gtggtgccca atggcctcgg cagtgacgtg
     8281 gccgagcggg agtcgaactt ggtccacgcg ctggacggcc tgttcgaaaa tggtctattt
     8341 cacatcaacg tgaacgtgct gaaccgcgag actttgctcg acgccatgga acatccggag
     8401 aagtatccgg agttgaccgt gcgggtttcg ggctacgcgg tgaacttcgt caagctgacg
     8461 cgcgaacaac aacaggaagt cgtgcggcgt acgttccacg gaagctcgtg agcaacgccg
     8521 gggcggaaac caagaccgag ctggggacca aaccagcgca cgaggaaggc cgcttcggat
     8581 acgtgcattc ggaacagacc gggtctgctt tggacgggcc cggtctgcgc accgtgttct
     8641 ggtagtcggg gtgcgagttc aggtgcacct attgccacaa cccggacacc tggaagttgc
     8701 acacggggcg gcggctctgc gtcgaagagg tgctcgcgga ggtcgccaag tacgccaagt
     8761 tcatgcgcgt ttcccgaggc ggggtgacgc tctcgggtgg ggagccgctg gtgcagtatg
     8821 cgttctgcat gaatctgctg cgcggagcgc gcgagctcgg ggtgcatacc gcgctggata
     8881 ccaacggctt ccacggcgcg cgaatgacgg acgacgatct tcagctcgtc
//
LOCUS       DASTKU010000897         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_844401, whole genome shotgun sequence.
ACCESSION   DASTKU010000897 DASTKU010000000
VERSION     DASTKU010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844401"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1413
                     /locus_tag="VFQ61_31505"
     CDS             <1..1413
                     /locus_tag="VFQ61_31505"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9959074.1"
                     /translation="RNVIVMEYMEGQSLSRLNRRAELAQQPLPQIMHLAILTHVLEGL
                     HYAHELNSYDGLPLELVHRDISPQNVFVTYDGQVKILDFGIAKTVSSSRETGLGLLKG
                     KISYMAPEQMQSEHLDRRADIYAAGCMLWAAAAGRKLWKDIAESQILDNVSAGRVPLP
                     SSVNPNCPAELERITMRALAFRREDRYATAAEMQAELEAFIEPWGMPRPRELGALVSN
                     LFANDRQALRSHVEQQLAALSSETQPDIVAFGANEAEEMPWLSDTRRGPIDSSVGLTA
                     TSPPVLKRRRWPWVSLVAVIAGAATALALPWVPRAVLAQFGLAEVKLKSDTHASRTND
                     SSSGRIPASGHLPPRLIVPIPEAYVADYRSEPAPGPVSSMGSSLGASAGPARPTALQP
                     TPVFPAKGAKPSSAPSPASAPVPPSTGGVSLDVRGTTRPEAAPASNAPAANSDASRAR
                     CNNPFFIDADGIKRLRPECY"
     gene            complement(1455..>2729)
                     /gene="zwf"
                     /locus_tag="VFQ61_31510"
     CDS             complement(1455..>2729)
                     /gene="zwf"
                     /locus_tag="VFQ61_31510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET9959075.1"
                     /translation="FPVVGVAKSGFTREMLVERARASLREHAGGVDEAAFSKLAARLC
                     YVDGDYNDSATFSALREKLEGAVRPTHYLAIPPSMFGTVAEQLHAAGLSEGARVVVEK
                     PFGRDLESARKLNHILHSYFPEQNLFRIDHYLGKEAVQNILYFRFANAFLEPVWNRQF
                     VENVQITLAEDFGVTGRGKLYEETGVIRDVIQNHLFQVVSYLAMEAPSSLYPEAIRDE
                     QAKVLRTVRALNAKDVIRGQFRGYREERDVASDSCVPTYAALRLYVDSWRWQGVPFFV
                     RAGKGLHKTVTEVSVELKNPPQVVFAEPPPSQGNTVRFRLNPEVAIAVGARTKRHGDK
                     MVGNPVELSVTEEPAQGHEGRMGPYERLLQDAMNGDTTLFARQDVVEAAWAIVDPVLH
                     EPWTLYEYEMGTWGPSEADELVREVGGWNTPQ"
BASE COUNT          502 a          879 c          826 g          522 t
ORIGIN      
        1 cgtaacgtca tcgtgatgga gtacatggaa ggtcagtctc tatcgcgctt gaatcgacga
       61 gcggagttag cgcagcagcc gcttccgcag atcatgcatc tagcgatact gactcacgtg
      121 ctcgaggggc tgcattacgc gcacgaactc aactcgtacg acgggctgcc gctcgagctc
      181 gtgcatcgag acatctctcc gcaaaacgta ttcgtgacct acgacggtca agtcaaaatc
      241 ctcgatttcg ggatcgcaaa gaccgtctcc tcgtcccgcg agaccgggct cggtctgctg
      301 aagggcaaga tttcctacat ggcgccagaa caaatgcaga gcgagcattt ggatcggcga
      361 gcagacattt atgcggcggg ctgcatgctg tgggcggcgg cagcgggacg caagctttgg
      421 aaagatatcg ccgagtcgca gatcctcgac aatgtctctg ccggtcgcgt tccactgccg
      481 agcagcgtga atccgaattg tcccgccgaa ctcgagcgaa tcacgatgcg ggcgttggct
      541 tttcgccggg aggatcggta cgcgacggcc gcagagatgc aagccgagtt ggaggccttc
      601 atcgagccct gggggatgcc ccgcccccgt gagctcgggg cgttggtctc gaacttattc
      661 gcgaatgatc gtcaggctct ccgaagtcac gtcgagcagc agctcgcggc cttgagctcg
      721 gagacgcaac cagacatcgt ggcgttcggc gccaacgaag cggaagagat gccatggtta
      781 tcggatactc gccgcggtcc aatcgactcc agcgtggggc tgacggcaac atcaccaccc
      841 gtgctcaaac gccggcgctg gccttgggtg tcgttggtgg ccgtgattgc cggcgctgca
      901 actgctttgg cgttaccctg ggttccgcgc gcggtgttgg ctcagttcgg actcgctgag
      961 gtcaagctca agtccgacac gcacgcctct cgcaccaacg actcgagctc gggccgcatc
     1021 cctgctagcg gacatttgcc gccacgtctg attgttccaa tccccgaggc ttacgtcgcg
     1081 gattacagat ccgagccagc gccaggtccg gtgtcgagta tggggtcgag tctgggagct
     1141 tcggcagggc cagcgcgtcc tacggctctg cagcccactc cggtgtttcc ggcgaaaggc
     1201 gccaagccaa gcagtgcgcc gtcccccgcg tcggctccgg ttccgccttc gacgggcggc
     1261 gtttcgctcg atgttcgcgg cacgactcga ccggaagccg cccccgcgag caatgcgccc
     1321 gccgccaact ccgacgcttc ccgcgcgcgc tgcaacaacc cgttcttcat cgatgcagac
     1381 ggaatcaagc gtctccggcc tgaatgctac tgagcagtca gcgggcgaag gcttcgaccg
     1441 agcgtaggtg gtctttactg cggcgtgttc cagccgccca cctcgcgcac caactcgtcg
     1501 gcttccgaag ggccccacgt gcccatctcg tactcataga gtgtccacgg ttcgtgcagc
     1561 accggatcga cgatggccca ggcggcctcc accacgtcct gacgcgcgaa caaggtcgtg
     1621 tcgccgttca tcgcgtcctg caatagccgt tcataaggcc ccatgcggcc ctcatggcct
     1681 tgggcgggct cctcggtcac ggagagctcc acggggttcc cgaccatctt gtcgccgtga
     1741 cgcttcgttc gagcgccaac cgcgatcgca acttccgggt tcaggcgaaa ccgcacggtg
     1801 tttccttggc tcggaggggg ttcggcgaat acgacctgcg gtgggttctt cagctccacg
     1861 ctcacttcgg tgacggtttt gtgcagaccc ttacctgctc gaacgaagaa cggcactccc
     1921 tgccagcgcc aagaatccac gtacaacctc agcgcggcgt acgtcggaac acaggagtcc
     1981 gatgcgacgt cgcgctcttc gcgataacct cggaattgac cgcgaatcac gtccttggcg
     2041 ttgagggcgc gaacggtgcg caacaccttc gcttgctcgt cacgaatggc ctccggatac
     2101 aaactcgagg gcgcttccat ggccaagtaa ctcacgacct ggaacagatg attttgaatg
     2161 acatctcgaa tcacgccggt ctcttcgtac agcttgccgc gccccgtgac gccgaagtcc
     2221 tctgccagcg tgatctgaac gttctcgacg aactgtcggt tccaaaccgg ttccaggaag
     2281 gcgttggcga accgaaagta gaggatgttc tggactgctt ccttgccgag gtaatggtcg
     2341 atgcggaaca gattctgttc tggaaaatac gagtggagaa tgtggttgag cttgcgggcg
     2401 gactcgagat ctcgcccgaa cggcttctcg acgacgacgc gcgcgccctc ggacaatccc
     2461 gccgcgtgca gttgctccgc gacggtgcca aacatgctcg gggggatcgc cagatagtgg
     2521 gtcgggcgca ccgcgccctc cagcttctca cgcagggcgc tgaacgtcgc cgagtcgttg
     2581 tagtcgccat ccacgtaaca caggcgcgcc gcgagtttcg aaaacgcggc ctcgtctacg
     2641 ccgcccgcat gctcgcgcag gctcgcgcgc gcgcgctcga ccagcatctc ccgcgtgaat
     2701 cccgatttgg cgactcccac gaccgggaa
//
LOCUS       DASTKU010000898         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_844461, whole genome shotgun sequence.
ACCESSION   DASTKU010000898 DASTKU010000000
VERSION     DASTKU010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844461"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..442
                     /locus_tag="VFQ61_31515"
     CDS             212..442
                     /locus_tag="VFQ61_31515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959076.1"
                     /translation="MHPFLRPDRLLFGACLVLAGCSAAPVLPAQPPPQEPQTRLYAPI
                     RRHITDPPEIQAPPPAYGNKIVMAERAPHSQL"
     gene            complement(479..1681)
                     /locus_tag="VFQ61_31520"
     CDS             complement(479..1681)
                     /locus_tag="VFQ61_31520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959077.1"
                     /translation="MAHSSRALSFAALALAAFSQIACGTGFDPGSELKSLRVLGVQKS
                     IPYPQPGDDVTLSMLWHDGRSAQEGQPVPGRLDNSNPSQRNVQVMWFPPCLNPAGDLY
                     QTCFADPRLFLSEGVPPLTDRAAYMRDRFTFQVPAGDKVFQGREPLPGQPKYGLFYVF
                     FALCAGTLELQSDSPLPRCVLDTGESLEPDADGLGGSKEFVIGYSTVYVFATKDGQEF
                     SNENPTFAAPKFRIGGREYEPDCMDPLPAELPAGSTLSSCITPEGGQLPLPNLSDDEP
                     CDEDRVACVKPCEADGEPGDCPAISIGPALDRDVVEREKDTVSAAYFNRPNFNEQMWI
                     NYYTDHGGLKSEVRLLNDATEGFNPKYRTDFYAPKAGPSTLWAVVHDNRGGQAFMRKT
                     ILVKPDAP"
     gene            complement(1683..>2189)
                     /locus_tag="VFQ61_31525"
     CDS             complement(1683..>2189)
                     /locus_tag="VFQ61_31525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959078.1"
                     /translation="ETLLKYKPGKRFFGWVTYTLSRSVRQSRAGEPERLFEYDQTHNL
                     TVLGSYRLGRGWEFGARFRLVSGPLATPLAPPPSLPAVYAADAGAYTPLEGKQFSRRL
                     PLFHQLDVRLDKRWQFRDWRFSTYLDVRNAYSNSAIEGLAYNFDYSQETYQTGIPIIP
                     SLGVRGEF"
BASE COUNT          415 a          689 c          678 g          407 t
ORIGIN      
        1 ctagcgtcgt cgatttggcc gagcactgca atcgactctg aacgatcgca tgcaagtcga
       61 aacgcgggcc gagacgccgg ccagccgctc tgtagcggta tgagtcacat gctgcgccgc
      121 ggcggagctc ttacagtccc cggagcggaa gccgatagat gactaagtcc ggtatcgagc
      181 gcccggccgg gcgggtgcta tcttatttaa ggtgcacccg tttttgcgac ccgaccgtct
      241 cttgttcgga gcgtgcttgg tgctcgctgg ttgtagcgcc gcaccggtcc tgcccgcgca
      301 gcctccgcct caggagccgc aaaccagact ttacgcaccg attcgtcgcc acatcaccga
      361 tcccccagag atccaggcgc ctccccccgc ctatgggaac aagatcgtga tggcggagcg
      421 agcacctcat tcccagctct gattccgtta cggagccgct ggttgctggc cgactacgtc
      481 acggtgcgtc gggcttgacg agaatcgtct tacgcatgaa agcctgcccg ccgcgattgt
      541 cgtggacgac ggcccagagc gtgctggggc cagccttcgg ggcgtagaag tccgtgcggt
      601 acttcggatt gaacccctcg gtcgcgtcgt tgagcagacg gacctcgctc ttcaatccgc
      661 cgtggtccgt gtagtagttg atccacatct gctcgttgaa gttggggcga ttgaagtacg
      721 ccgccgatac ggtgtccttc tcgcgctcga cgacgtctcg atccagcgct gggccgatcg
      781 agatcgcagg gcaatcgccg ggctctccgt cggcctcaca aggcttcacg caggccactc
      841 gatcttcgtc acacggctcg tcgtcgctca gattgggcag aggcagctga ccaccctcag
      901 gggtaatgca agagctcaag gtcgagcccg cgggcaactc cgcgggaagc ggatccatgc
      961 agtctggctc gtactcacga ccgccaatcc ggaacttcgg cgccgcgaag gtcgggttct
     1021 cgttactaaa ctcttggccg tccttggtcg cgaagacgta gacggtcgaa tagccgatca
     1081 cgaactcttt ggagccaccg aggccatcgg cgtcgggctc caagctttcg ccggtatcca
     1141 acacgcaacg cggcaggggc gagtccgact gtagttcgag cgttccggcg cacaacgcga
     1201 agaacacata aaacagacca tacttcggct gccccggtaa cggttcacgc ccttggaaga
     1261 ccttgtctcc cgcaggcacc tggaacgtga aacgatcgcg catgtacgcc gctcgatcgg
     1321 tgagtggtgg cactccttca ctcaggaaca gcctcggatc ggcaaaacac gtctgataca
     1381 agtctcccgc cgggttcagg catggaggaa accacatcac ctggacgttg cgctgactcg
     1441 gattgctgtt gtccaaacgg cccggaacgg gctgcccttc ctgggcggac cgaccatcgt
     1501 gccagagcat cgagagcgtc acatcgtcgc cgggttgtgg gtacgggatg ctcttttgaa
     1561 cacccaaaac tcgcagagat ttgagctcgc ttcccggatc gaaacccgtg ccgcaggcaa
     1621 tctgtgagaa agcggccagc gcgagcgccg caaacgagag ggcacgtgag ctatgggcca
     1681 tgtcagaact ctcctcgcac gccgaggctc gggatgatgg gaataccggt ctgataggtt
     1741 tcttggctgt agtcgaagtt gtaggcaagg ccttcgatcg ctgagttgct gtacgcgttg
     1801 cgcacgtcga gataggtcga gaagcgccaa tctcggaact gccagcgttt gtcgaggcgc
     1861 acgtcgagct ggtggaacaa gggcaagcgg cggctgaatt gcttgccctc gagcggggtg
     1921 taggcgcctg cgtcggccgc gtacacggcc ggcaaagacg gcggcggcgc gagcggcgtc
     1981 gccagcgggc ccgagacgag ccggaagcgc gccccgaatt cccaaccgcg gcccaagcgg
     2041 taactcccga gcaccgtcaa gttgtgggtc tgatcgtact cgaacagccg ctcgggctct
     2101 cccgctcgcg actgccgcac gctgcgcgag agcgtgtacg tcacccaccc gaagaagcgc
     2161 ttacccggct tgtacttcaa gagcgtctc
//
LOCUS       DASTKU010000899         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1069067, whole genome shotgun sequence.
ACCESSION   DASTKU010000899 DASTKU010000000
VERSION     DASTKU010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069067"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFQ61_31530"
     CDS             <1..352
                     /locus_tag="VFQ61_31530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 11 protein"
                     /protein_id="HET9959079.1"
                     /translation="YYILDDWGDTKPGGTASDGTPRTRVGTIDVDGETYDVWMKTRMN
                     KPAITGNNQTFDQYFSIRRTARQCGSISVSEHFRQWEKLGLQLGKLVEATLLLEAQDS
                     TGTIEFTTAAITVK"
     gene            complement(430..1002)
                     /locus_tag="VFQ61_31535"
     CDS             complement(430..1002)
                     /locus_tag="VFQ61_31535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959080.1"
                     /translation="MRSSNSVLGILGMVLTAAWLWSLAPTAHESLVAPQLQTSPAPES
                     PGPRQQLARQEPQMPPLPSQESRSLEKARLASYAEATRIAERERRLDTLDWTAPRPNP
                     SPQDQRVLSKSERALPPGNRAQQLALIAKRIQQRIEILEHPRSGERASAFGRDTPEGI
                     ALLERMRRRLGELAAERDTLLLTERAPQPK"
     gene            complement(1017..2120)
                     /locus_tag="VFQ61_31540"
     CDS             complement(1017..2120)
                     /locus_tag="VFQ61_31540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959081.1"
                     /translation="MKLRCAYWLAFALGAGCASDTQDRPNGAERVVDAPEQRGSVTLA
                     ILNANRIGGSYSYRGETLRFEGEALDSAYFVTLEVRGMILSATIDASGASDFDAFKRA
                     DGTDTAMTAEDRALVHALDSALTEISGEQASELPALAMFNHALALWSDYPSTIPLRRA
                     YFARAERTSSAQNLCSRVNKPGQGEAFSPIWTWASHDCFKVKGLDTNDCGARSGGCAY
                     GDDSSTVENVFMSMHPKGGYGDGRYYGDTASTFEAYEPDHDSNVEFGYGNCFGRCGAD
                     CGGSSQFTQACLDHDLCVTFGHWTWNPLSACDDEFIEAAIDYLAEPDCGGQLAIDYDW
                     AGSPYDGKCSESWNSSNDGCDVGCQFVDGDCFR"
     gene            2499..>3053
                     /locus_tag="VFQ61_31545"
     CDS             2499..>3053
                     /locus_tag="VFQ61_31545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959082.1"
                     /translation="MRWRHAGTCMVLAGAMGLTVAASATNPDDAFEQRIAGELSAIDP
                     SLVETFARANAARDKGDHRTAERLYAEVFEKAPGWYHAERRRCGELVALERRDEALPL
                     CRHAAEKEPSSANLVGLASAILHTPRGKQLYESEKDEAVELLRRAEALDPTDSQIALV
                     QCEAALARANLGELRDCRSRLERIA"
BASE COUNT          567 a          913 c          994 g          579 t
ORIGIN      
        1 gtactatatc ctggatgact ggggtgatac gaagccaggc ggaaccgcgt cagacggcac
       61 accgcgcact cgagtgggca ccatcgacgt cgatggggag acttacgatg tatggatgaa
      121 aacccgcatg aataagccgg ccattaccgg caacaaccag accttcgacc agtatttcag
      181 tatccggcgg acggcgcggc agtgcggaag catctccgtc tcggagcact tccgccaatg
      241 ggagaagctc ggtttgcagc taggaaagct cgtggaagcc acactcttgc tcgaggcaca
      301 agacagcacc ggaaccatcg aattcaccac ggccgcgatc accgtcaaat gagctggcgc
      361 gaatcgatag tatcagaccg tcggcgatcg gccgcggtcc gacgcacgaa ccttttcagc
      421 tcggtgccct cacttcggct gtggagcgcg ctcggtcagg agtagcgtgt ctcgctctgc
      481 cgcgagctcg cctaagcgtc gacgcattcg ctcgagcagc gcaatccctt ccggggtgtc
      541 gcgcccaaac gcgctcgcac gttcgccgct tcgcggatgc tccagaattt cgatacgctg
      601 ctgaatgcgc ttcgcgatga gtgcgagctg ctgtgcccta ttgcccggcg ggagcgctcg
      661 ttcagacttg ctcagcacgc gctggtcttg gggcgaagga ttcggtcttg gcgcggtcca
      721 gtcgagagta tcgaggcgtc gctcgcgctc ggcaatgcga gttgcttctg cgtaggaggc
      781 gagccgcgcc ttctcgagag aacgactctc ctgggagggt aggggtggca tctgcggttc
      841 ttggcgcgcc agttgttggc gaggcccagg cgactccggc gctggcgagg tctgcaattg
      901 cggcgccacc aaggactcgt gagcggtggg tgcgagggac cagagccaag ccgcggttaa
      961 caccatgccg aggatgccca atactgagtt cgaagagcgc atatcaagga tcctcctcag
     1021 cggaagcagt ccccgtctac gaattggcaa ccgacatcgc agccatcgtt ggagctgttc
     1081 catgactccg agcatttgcc atcgtagggg gagcccgccc agtcatagtc gatcgccagt
     1141 tgcccaccgc aatcgggctc cgcgaggtag tcgatggccg cctcgatgaa ctcgtcatcg
     1201 cacgcggaga gggggttcca tgtccagtgc ccgaatgtca cgcagagatc gtgatccagg
     1261 caagcctggg tgaactggct gctgccaccg cagtccgcgc cgcaacggcc gaagcagttg
     1321 ccgtacccga actcgacgtt cgagtcgtgg tcgggctcgt aggcttcgaa ggtgctcgcg
     1381 gtatcaccat agtagcgacc gtcgccgtag ccgcccttcg ggtgcatgct catgaacacg
     1441 ttttcgaccg tcgagctgtc atcgccgtaa gcgcagcctc cggagcgggc accgcaatcg
     1501 ttcgtgtcga gacctttgac cttgaagcag tcgtgggacg cccatgtcca aatgggcgag
     1561 aatgcttcgc cttggccggg cttgttcact cgactgcaga ggttctgcgc gctgctcgtg
     1621 cgctcagcgc gcgcaaagta cgctcggcgg agtgggatcg tgctggggta gtcgctccag
     1681 agggcaagcg catgattgaa catggccaag gcgggcaatt ccgaggcctg ctccccggag
     1741 atttcggtga gcgccgaatc caaggcgtgg acgagcgcgc gatcttcagc agtcatggct
     1801 gtatcggtgc catcggctcg tttgaaggcg tcgaagtccg acgcccccga agcgtcgatc
     1861 gtggcgctca ggatcattcc gcgcacctcg agcgtcacga agtatgcgga gtcgagcgcc
     1921 tcaccctcga aacggagtgt ttcaccgcga tagctgtagc tgccgccaat gcggttcgcg
     1981 ttcagaatgg ccagcgtgac cgatcccctt tgttcgggcg cgtcgacgac ccgctccgcg
     2041 ccgttcggtc gatcctgcgt atcgctcgcg caacccgcgc ccagagcaaa cgcgagccag
     2101 tacgcgcatc tcagcttcac gtgagacgtc cgctgtgtac accgacctga ctggaggagc
     2161 gtgcccctcc ctgggcagtg gaaataattc cgattcttgt ctctcgtgtt cacgactgat
     2221 gacctcgtgg ctcgctcgtt attccagcaa ctcgcgtgcc tacaagctgt cgaggtctgg
     2281 gaagaggcaa aatgaacagt ctcgcgggag ggattgggcc ggctttggcg cagttggtcg
     2341 agagtgggcc cgattgactg catgtgagtt gcgggccagc tgaaaagtgt cagtgcgctc
     2401 ccgaccggtc cctccaacgc gtatgccaac tggcagttta ggacgagatg cgcgcgaatg
     2461 caggccgtgc ctaggccctc ccatgataag tgtgaaacat gcgctggaga cacgcgggga
     2521 catgcatggt gttggcggga gctatggggc tgacggtcgc ggcaagtgcc accaatcccg
     2581 atgatgcttt cgagcagcgg attgcgggcg agctttcggc tatcgatcct tcgctcgtgg
     2641 agacgtttgc gcgcgcgaat gcggcccggg acaagggtga tcacaggact gccgaacgcc
     2701 tgtacgccga ggtcttcgag aaagcacccg gctggtatca cgcggagcgt cgccgctgcg
     2761 gtgagctcgt ggcgttggag cgtcgggacg aggcgctccc gctgtgccgg catgcggccg
     2821 aaaaggagcc gtcgtccgcc aacttggtgg ggctagcctc ggccattttg cacacgccgc
     2881 gaggcaaaca actatacgaa agcgagaagg acgaggccgt cgagttgttg cgacgcgccg
     2941 aagctttgga cccgactgat tcgcagattg cactagtgca gtgcgaagcg gcgctcgcgc
     3001 gcgcgaacct gggcgagctg cgcgattgtc gctcccgttt ggagcggatc gcg
//
LOCUS       DASTKU010000900         4021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1739188, whole genome shotgun sequence.
ACCESSION   DASTKU010000900 DASTKU010000000
VERSION     DASTKU010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739188"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFQ61_31550"
     CDS             <1..268
                     /locus_tag="VFQ61_31550"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9959083.1"
                     /translation="VFDRLAAHKTVATRLAGDKRFEFVFLPAYAPQLNPDEWVWRYAK
                     HTLLGGCCPWGYDDLQNAVHSALSSFKSHSDLLASFFRGARLSL"
     gene            379..1515
                     /locus_tag="VFQ61_31555"
     CDS             379..1515
                     /locus_tag="VFQ61_31555"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HET9959084.1"
                     /translation="MLYSEFTSTIQSVRSPAWILTYTTVDVPLAQKLAAIAKAYPGVP
                     VFGCTSFQGVFSPLGFTRGMHALIASAEDGVRAATALRAVGSSRARTEARAAAGEIRK
                     ALRDGTHCILMHATPGFEERVLEGIEEAYSGSPPPVYGGSAADDDMSGKWQVYSGGSV
                     LNEGFVLAGFSSPKRVMGSFVGGYTPTNTRGVVTSVLGRMVQTIDHKPAAQVYNEWTY
                     GGIQDQLARGGVVLQATALRPVGRLVDKVGAVPRYLLSHPHQVMPDGSLWFFSEMREG
                     DELVLMLGTEAALVERTSQVATRALGSAQGARNLSGGILVFCGGCVSAMPGKTNEVSH
                     AFRQQLRSAPFVGASTFGEQGCFTGRNAMNRHGNLMCDAVLFEA"
     gene            1782..2849
                     /locus_tag="VFQ61_31560"
     CDS             1782..2849
                     /locus_tag="VFQ61_31560"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9959085.1"
                     /translation="MTSPWALGLPILPGQPLVLVFMEEWPRAILPCESGRRAAELERL
                     RAELRGLGASLIAVTNSSLLCFRPDDEAVILPSVSGRPFGSVRDLADRYEVDPDRVAA
                     GELCVLVLDGAGAVRDRESSMRPSDPLGAVLDALAMAGRTVRETLPVSHLSRRELVIA
                     SLAGAFALVLANACKPNPAGTPSGPAPTSSVSGPPEVAKELPVTLLVNGQTHALSLEP
                     RVSLLDALRERLGLTGTKKGCDHGQCGACTVLVDGRRVNACLTLAIQVDGVPVTTIEG
                     LAKGEELHPMQAAFVEEDALQCGYCTPGQIMSAMGLLKENRAHTPAEIREHMSGNLCR
                     CGAQVNIVTAIVRARKAGAAV"
     gene            2846..3844
                     /locus_tag="VFQ61_31565"
     CDS             2846..3844
                     /locus_tag="VFQ61_31565"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET9959086.1"
                     /translation="MKAFDYTKPTSLEEATQRGSAAGARFIAGGTLLVDLMRLNVERP
                     DLLVDLGKLALTRGDLRQIELDATGLRIGALVRNTDLAYHAVVLDKFPVLSEALLSGA
                     SPQIRNMASVSGNLLQRTRCAYFRDTAVKQCNKRSPGSGCAALDGFSRMHAILGVSPN
                     CIAAHPSDMCVALAALDAVVVIRGSAGEREVPLSDFHVLPADHPEVESVLGHGEIVTH
                     VRVPSSPFAKHSAYVKARDRASFAFALASAAAALELQGTVIRSARIALGGVGTKPWRC
                     AASEAKLVGGPANRAAFESAAKLGLAGAKTTKDNAFKVKIAERAVVRALEIARSKA"
     gene            3841..>4021
                     /locus_tag="VFQ61_31570"
     CDS             3841..>4021
                     /locus_tag="VFQ61_31570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959087.1"
                     /translation="MNGPVVGKGLDRADARLKVTGKAQYAAEVPAANLTYVVIVSSQV
                     GKGRVTKVETGAAERH"
BASE COUNT          703 a         1160 c         1368 g          790 t
ORIGIN      
        1 cgtgttcgac aggcttgcgg cgcataaaac ggtcgccacg cggctcgctg gggacaaacg
       61 ttttgagttc gtcttcctgc ccgcctacgc cccgcagctg aatccggacg aatgggtctg
      121 gcgctacgcc aaacacacgc tactcggcgg ctgttgcccc tggggctatg atgatttgca
      181 gaacgccgtt catagtgcac tgtcgtcttt caagtcccat tctgatctgc ttgcgagctt
      241 ctttcgtggc gcccgactgt ctctttagct caatgaattg tcagtaacgc tagcccgagc
      301 ggatatgaca cctgatctgg gggtgatgga ctcaggcgct ctttccgtgc agtatgcgac
      361 gcaattgggg caagctcgat gctttatagt gagttcacca gcacgattca gtccgttcgt
      421 tcacccgcgt ggattctgac ctacaccacg gtggacgtgc cgttggcgca gaaattggca
      481 gcgatcgcca aggcttaccc gggggttccg gtgtttgggt gcacctcgtt tcaaggggtg
      541 ttttctccgc tcgggttcac gcggggcatg cacgccctga tagctagtgc ggaggatggc
      601 gtgagagccg cgaccgctct gcgcgccgta ggttcttcgc gggcgaggac ggaagctcgc
      661 gcggccgccg gggaaattcg caaagcgcta cgcgacggaa cgcactgcat cttgatgcac
      721 gccacacccg gtttcgaaga gcgcgtgttg gaaggcatcg aggaggccta ctccggatca
      781 ccaccgcccg tgtacggggg cagtgccgcc gatgacgaca tgagcggtaa gtggcaggtc
      841 tattcgggtg gctcggtgct caacgagggc ttcgtgctgg cggggttttc gtccccgaag
      901 cgggtcatgg gatctttcgt tggcggttac actccgacca acacacgcgg ggtcgtgact
      961 tcggtgctcg gacgcatggt ccagaccatc gatcacaagc ccgcggcgca ggtttacaac
     1021 gagtggacct acggcggaat tcaggatcaa ctcgccaggg gcggcgttgt attacaggcg
     1081 accgcgctac gcccggtggg caggctggtc gacaaggtcg gagcggttcc gcgctatctg
     1141 ttgtcacatc cccatcaagt catgccggat ggttcgcttt ggttcttcag cgaaatgcgt
     1201 gaaggcgacg agctggtgct gatgctcggt acggaggcgg cactggtcga gcgtaccagt
     1261 caggtcgcga cgcgcgcgct cggttccgcg cagggagctc gcaacctatc aggcggtatt
     1321 ctagtttttt gcgggggatg cgtgagcgca atgcctggca aaaccaatga ggtgagccac
     1381 gcttttcgcc agcagctgcg gagtgcgccg ttcgtcgggg cgtccacgtt cggagagcag
     1441 ggctgcttta ctgggcggaa cgcgatgaat cgacatggca acttgatgtg tgatgccgtg
     1501 ttgttcgagg cttgaaggcg cacccaggag cgacaggcgt ggatgcacgt gagttccatt
     1561 cacgtgcaaa gcaccgggcg gtgaaacccc gctcatccgg attctatgtg gcttcctctg
     1621 gaatcggcgc cgcttccacg ccacaccggg gcaacaatac cgcctccggt cttcgcgttg
     1681 gaccacgatc tcgatgcggg tccgaggtcg attgaatgat ctctggttag acatctgtca
     1741 agactccgca gaggtatgca gcttgcttcg gcgcggggcc gatgacgtcg ccttgggccc
     1801 tcggcctccc gatcttgcca ggccaaccgc tggtgttggt gttcatggaa gagtggccgc
     1861 gggccatttt accgtgtgaa agcggccgcc gcgccgcgga gttggagcgg ctccgtgccg
     1921 agttacgcgg gttgggggct tcgctcatag cagtgacgaa cagctcgctg ttgtgctttc
     1981 ggcccgacga cgaagccgtt attttgccga gtgtttccgg aaggccattc gggtccgttc
     2041 gggacttggc ggatagatac gaagtagatc cggatcgggt ggcggcgggc gaactgtgcg
     2101 tgctcgtgtt ggatggcgcc ggcgcggttc gggatcgaga gagctcgatg cggccttccg
     2161 atccgctcgg tgccgtgctc gacgctctcg cgatggcggg acgaacggtg cgtgaaacat
     2221 tgcccgtgag ccacctctcg cgccgcgagc tggtgatcgc gagtttggcg ggtgctttcg
     2281 cgctcgtgtt ggcaaacgcc tgtaagccga atcccgccgg cacaccgagt ggaccggcac
     2341 ccacgagctc cgtgtccggc ccgcccgagg tcgcgaagga gctgccggtg actctcttgg
     2401 tcaatggtca gactcatgca ctctcgttgg agccgcgtgt gtccttgctg gacgcgctgc
     2461 gcgagcgtct gggcttgacc ggtacgaaga agggctgcga tcacgggcag tgcggcgcct
     2521 gtacggtgct cgtcgatggg cggcgcgtga acgcctgcct cacgctcgcg atccaggtgg
     2581 atggcgtgcc cgtcaccacg atcgaaggct tggcaaaggg cgaggagctg cacccgatgc
     2641 aggctgcgtt cgtggaggaa gacgcgctgc agtgcggata ttgcacgccc gggcaaatca
     2701 tgagcgccat gggcctgctc aaagaaaacc gggcgcacac gccggcggag attcgtgagc
     2761 acatgagtgg aaacctgtgt cgctgcgggg cccaagtaaa tatcgtcacg gccatcgtcc
     2821 gggctcgcaa ggccggggcg gccgtatgaa ggccttcgat tacacgaagc ccaccagctt
     2881 ggaggaagcc acccaacgcg ggagcgcggc gggcgcgcgc ttcatcgccg ggggcactct
     2941 gttggtcgac ttgatgcgct tgaatgtgga gcgcccggat cttctcgtcg atctcggcaa
     3001 gctcgcgctc acccggggcg acctccgaca gatcgaactc gacgccacgg gactgcggat
     3061 tggcgcgctg gtgcgcaata ccgatctggc atatcacgcg gtcgtgctcg acaaattccc
     3121 ggtgctctcg gaggctttgc tttcgggcgc ttcgccgcaa attcggaata tggcgagcgt
     3181 gagtggcaac ctgttgcagc gcacgcgctg cgcgtacttt cgcgatacgg cggtaaagca
     3241 atgtaacaag cgctcaccgg gctccggctg tgcggccctc gatggcttca gcaggatgca
     3301 tgcgattttg ggggtgagcc cgaattgtat cgccgctcat ccttcggata tgtgtgtcgc
     3361 gctcgcggcg ctagacgcgg tcgtggtgat tagagggtca gcgggggagc gtgaggtgcc
     3421 actgagcgac tttcacgtac tgcctgccga tcatcccgaa gtggaaagcg tactcgggca
     3481 cggcgagatc gtaacccatg tgcgggtgcc gagttctccc ttcgccaagc actcggccta
     3541 tgtcaaagcg cgagaccgag catcgtttgc ctttgcgctg gcttcggcgg cggccgcctt
     3601 ggagctacaa ggcaccgtga ttcggagcgc gcgcattgcg ctgggaggcg taggcaccaa
     3661 gccctggcgg tgcgcggcga gtgaagcgaa gctcgtaggg gggccggcaa atcgggcggc
     3721 cttcgaaagc gccgccaagt tgggcttggc gggagcgaag acgaccaagg acaacgcgtt
     3781 caaagtgaaa atcgccgagc gagccgtggt gcgcgctctg gagatcgccc ggagcaaagc
     3841 atgaacggac ccgtcgttgg caaaggtttg gatcgcgcgg acgcacgcct caaagtgact
     3901 ggcaaagccc agtacgcagc cgaggtgccg gccgccaatc tgacctacgt ggtgatcgtc
     3961 agcagccaag tcggaaaggg ccgcgtgacg aaggtggaga cgggcgcggc tgagcgccac
     4021 c
//
LOCUS       DASTKU010000901        16574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_398200, whole genome shotgun sequence.
ACCESSION   DASTKU010000901 DASTKU010000000
VERSION     DASTKU010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16574
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398200"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1111)
                     /locus_tag="VFQ61_31575"
     CDS             complement(113..1111)
                     /locus_tag="VFQ61_31575"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HET9959088.1"
                     /translation="MNLGTRYLGLNLAHPFVLGASPLGHELDTIRRAEDAGAAAVVLH
                     SLFEEQILLENGFIEQVMGRHELSHAEASDYFPSSDEFALSSEEYLEHISKVKKHVSI
                     PVIASLNGVTNHGWLEYATLCEQAGADALELNVYQLATNATDTSEAIEQRTLEMVKTV
                     RGRTGLPLAVKLSPFYTSLCHFAHRLEDAGANGLVLFNRFYQPDIDPEALDVRSKLEL
                     SNSSELLLRLRWLAVLSPMVRGSLAVTGGVHTGIDAIKALMAGAHAVQVVSEVLMRGP
                     ERFGELVSELRVWLEQHEYGSVSQLQGSMNLERCPDTGGYERANYMRMLRSYNRAG"
     gene            complement(1108..4803)
                     /gene="nifJ"
                     /locus_tag="VFQ61_31580"
     CDS             complement(1108..4803)
                     /gene="nifJ"
                     /locus_tag="VFQ61_31580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HET9959089.1"
                     /translation="MSAVVTQLKGQERSARPAVEGKPGPEVSHSLKTSHSLETLDGNQ
                     AAADVAYRLNEVIAIYPITPASPMGEFADEWAARGRVNIWGDVPSVTEMQSEAGAAGV
                     VHGALTSGALSTTFTASQGLLLMIPNFYKIAGELQPFVVHVAARTLATHALSIFGDHS
                     DVMACRATGFAMLAAASPQEAHDMACVAHAAALTSRVPFMHFFDGFRTSHELTRVSLL
                     SDDVLRAMIDERAILEHRRRALTPDRPSLRGTAQNPDTYFQAREAVNPYYAACPDIVE
                     RTLDHFANQTGRRYKLFEYVGHPEATDVIVAMGSGALLAQQVAAEKAARGERVGALLV
                     RLFRPFSARHLAAALPSTVERISVLDRTKEPGAAGEPLYLDVMAALFESPQRNMPRMV
                     SGRYGLSSKEFTPEMVEAVFANLASSRPRRHFSVGIVDDVTNESLTVPAPAPRGEDGV
                     FESVFFGLGSDGTVGANKNTIKIVSSESSLNVQGYFVYDSKKSGAVTISHLRFSEATF
                     QRPYLIREADFVGCHQFDFLETLDVVSRARHSGTLLVNSPFPADEFFQHVPLEVWEQV
                     REKNLNVYVVDAYAIASKAGLGRRINTVMQACFFALTNLIKPELAVGAMKHAIEESYG
                     KKGGELVRRNWAVLDNSVSGLSQVKVPEQAPHAQRRPPRIPGKAPDFVQRVTAAILEG
                     HGDLLPVSAFPVDGCWPTATARWEKRNIAAEIPVWDPSICVQCNKCALVCPHAAIRAK
                     VYDPAELAAAPPEFKSMTWKGGEFAGKGYTIQVAPEDCTGCHLCVEICPAKDKSNPRH
                     KSLEMAPQRPLRESEARNFEFFIGLPAMSRSRVRLDVKSSQLLDPLFEFSGACPGCGE
                     TPYVKLLTQLFGDRLLIANATGCSSIYGGNLPTTPYTVDGKGRGPAWANSLFEDNAEF
                     GFGMRVSVDQLTSSARSLLRKLQRELDPELVQELLTAAQDNDAQIDAQRARVERLRAA
                     LSGSEDPDAVRLLEAADYLVKKSVWLVGGDGWAYDIGFGGLDHVLASNRKINVLVLDT
                     EVYSNTGGQQSKATPLGAVAKYATLGKSVGKKDLGLLAMSYGHVYVASIAFGAKDAQT
                     VRALAEADAHPGPSLVIAYSHCIAHGYELQFGAQQQKLAVDSGVWPLYTYNPQRTEQG
                     ERPLVIDQQPGKAKVREYMNNEARFRMLEKTDKARFEALLVAAEHMAARRNRVYEELS
                     HIAPAPKESKAPAPTESRPSNGDGGKS"
     gene            5108..7000
                     /locus_tag="VFQ61_31585"
     CDS             5108..7000
                     /locus_tag="VFQ61_31585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9959090.1"
                     /translation="MRPRAMSSSFPAPPSFKSRYPKKLVSAAQAIRNLASGQRVLLGS
                     GAAEPVTLVNALVEHGQHLADTEVVHILTLGPAPYVAPEHQGRFRHTAFFIGPNTREA
                     IQQGRADFMPVFLSEIPNLIRSRRIRIDAVLIQVAPPDRHGYASLGVSVDVVRSAVDA
                     AELVIAEVNPNMPRTLGDSFVHVNRIDYLIEVDTPLLELLPEPLDDVAEEIGKNVATL
                     IPDGATLQTGIGRIPHAVLNALQGHHDLGLHTEMLSDTVIELVEAGVINGRRKNLCPG
                     KLVTSFVMGTQRLYEWVDDNPLIEMRPTEFVNDPTTIARNDNMISINSALAVDLTGQV
                     AADTLHGRFFSGIGGQVDFIRGAARSRGGKPIIALPACAENGTISRIQAALEPGAGVV
                     TSRGDVHYVVTEYGVADLWGKTVRERAMSLIAIAHPDFRPDLLNQAKQRHYVFPDQCV
                     PHGPYPGGTTRVVTLRDRRDVVIRPIRITDERALQELFYRLSSESVYQRFLHFKNKHP
                     HQEMLALVDHDYDSAVALVAAPVDDDTGRLVAMARYDVDPATHFADVAFVVDDQWQRV
                     GLGTLLFSRLGEVAKAKGLRGFTADVLSSNKAMLGVFQASGMRCETERDGNVTHLTMR
                     FSQSAV"
     gene            complement(7007..7963)
                     /locus_tag="VFQ61_31590"
     CDS             complement(7007..7963)
                     /locus_tag="VFQ61_31590"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET9959091.1"
                     /translation="MPRVVVLDGYTVNPGDNSWSELEQLAELSVYERTPPNLVIERSR
                     GCQVVITNKVPFDAATLEALPELRGICVSATGYNIVDLEAASRGGVAVCNVPAYSTAS
                     VVEHTFALLFELCRRVGLHDRLVHEGHWASSPDFAFWRTPQIELHGLRFGVVGYGSIG
                     SAVARVAQAFGMVVLSTPSRQRPPESFVTTANLDTVFEQSDVVSLHCPLTEQTRGLVD
                     EARLRSMKRSAFLINTSRGALVQELDLADALRDQTIAGAALDVLSQEPPPPDHPLTQL
                     QNCIITPHLAWTSLAARKRLLRTTVENVRGILQGKPINRVNR"
     gene            complement(8090..9967)
                     /locus_tag="VFQ61_31595"
     CDS             complement(8090..9967)
                     /locus_tag="VFQ61_31595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959092.1"
                     /translation="MSNRPTPIGGRYAFHGLLKSQQALPRWLAQDAAQGQQFVASLIP
                     EHRLKHVEAAQGANHPHLASVLQVIRHVEASAVPEGMGFEPGISVAVAEYVPGQTLHQ
                     FQAGKPLPPDKAVAWVLRLSEAVQVLHAQGAVHGAISPWSVIAVPDGRSIAPVLSMFV
                     APVIGAYLPPERLKGAADQESSDVWALHALLYTALTGVPPFVGADRALLTRQMLVGRL
                     APLSTHGVQEPALNEILIRGLNGEKRLRMTELAELIRCLDAWERNPKAMPPRRAPAVR
                     PRALEADSQRVPPEHDVLVVDGSTLPHDEGAVQLVPRPRRSMPPLPMPAVSVPIPPPL
                     PVSAAPTPPPLPVTAAPVPPPLPSASSQPSRPHSSGPTLEELSAQLLPALPQPPLGTV
                     RPAHISKRLSFNPFEKKRRVWPLVVGGAAGGGFCVYLLLSLTRSAGAPAAAANTATVP
                     RAEAPANASASAKPKVSAEARRDTCVTAYLPEVLPRGEANFAFLCEEGDFRETARRLF
                     AGARSERKDLGGAGFGWYELPAAAIVRKSCCEAAPPPFLPKTSGWCEQVETAVRRVAE
                     DSAKAGDVAPAARSFDKAVQCLFANHVPHSYSYERVPVEAQRRAFQQFLSLAAINEAR
                     R"
     gene            10799..12640
                     /gene="dauA"
                     /locus_tag="VFQ61_31600"
     CDS             10799..12640
                     /gene="dauA"
                     /locus_tag="VFQ61_31600"
                     /inference="COORDINATES: protein motif:HMM:NF008660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HET9959093.1"
                     /translation="MKTSLQRLTRKIPRLPSSLASELGRLPLAFALRQVLTTGYGVNA
                     FKADVLAGAVVAIVALPLSMALAIAVGVPPQHGLYTAIVAGGSVALLGGSRFQVTGPT
                     AAFIVVLAPIVGKYGLPGLLTAGLLSGLILVLMGFARLGRWIEYIPHPVTTGFTAGIA
                     TVIFILQLKDALGLTTGALPDHFPEKVVALWHARGTASLPSVCVAAFTLLVLLGFPRL
                     TKRVPAPLVAIVSSSILVAVLDAIAPNLDIATVGSRFHTELNGKLIPGIPRSGPTLAA
                     PWAPHALNLEFVRDLLPSAFAIAMLGAIESLLSAVIADGMTGTRHEPNSELTALGIGN
                     ILTPLFGGIPATGALARTATNVRSGAKSPIAAFTHAVLVLLAILIAAPWVAYVPMASL
                     AALLMLVAWNMSEVRHAWHVVRVAPRSDVTVLITCYALTVFFDMVIAITFGVLLAAVL
                     FMRRMAELTQTRVLHGSSEGNSVRDTEQLPAAIAVYEILGPLFFGAAQRGMAALDALS
                     DRVRIVVLDLSRVPVIDASGLVALESALERLRRARRFVILGGPLPEPKAVFDRAELEK
                     HHENILLTESPGEAVAMAKTLVLLNPEWDVAAKTHLGGLSERPQRVL"
     gene            12887..13306
                     /locus_tag="VFQ61_31605"
     CDS             12887..13306
                     /locus_tag="VFQ61_31605"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9959094.1"
                     /translation="MTDFPALVRDVMTKDVVTLDRNDKLLVADDLMRLGRIRHLPVVD
                     EGGNLAGIVSQRDLFHSGLIKALGYGAHAQRQALDMVVVKEAMRSDVVTTTPETLLQE
                     AASVMLERKIGCLVVLEGGRIAGILTESDFVKYVSRK"
     gene            15058..15318
                     /locus_tag="VFQ61_31610"
     CDS             15058..15318
                     /locus_tag="VFQ61_31610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959095.1"
                     /translation="MAPGPKPRAAGGPARAKGAKRSPEELEELTTQLLEYIKNNPGQR
                     IEQIAEGLGTSTKELNLPAKKLLNTKAIKTKGHKRATQYLPR"
     gene            16211..>16574
                     /locus_tag="VFQ61_31615"
     CDS             16211..>16574
                     /locus_tag="VFQ61_31615"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET9959096.1"
                     /translation="MAAFGLILLCQSLAVAAEPVSRSHVVYDGQRLGSIAKRYGVSVE
                     AICGANDMRPTDRLKPGQRLVIPNRGDRDGSRARAANSSMTRRRSATEPQDGGPATHR
                     VESGQRLESIARRYGVKVE"
BASE COUNT         3152 a         5173 c         5092 g         3157 t
ORIGIN      
        1 ggcggcatcg atcccgctcg ctcaacccgc ctccatcctg cagcgtgacg ggcagcatgg
       61 cgccccagcg ctgcgacgaa gactgcgggg gcgcctcgcc aacggctgca gattagcccg
      121 ctcggttgta cgagcgtagc atacgcatgt agttcgctcg ttcatagccg ccggtatcgg
      181 ggcaacgctc gaggttcata ctgccttgca gctgggacac tgaaccgtac tcgtgctgtt
      241 cgagccatac acgaagctca ctcacgagct caccaaaacg ctccggtccg cgcatcaaga
      301 cttcggacac cacctgcacc gcgtgggcac ccgccatcag ggctttgatc gcgtcgatac
      361 cggtgtgaac gccacccgtg accgcgaggg agccccgcac catgggcgaa aggacggcga
      421 gccaacgcag acgcaacaac aactccgacg agttggataa ctccagcttc gagcgcacgt
      481 ccaaggcttc gggatctata tcgggctgat aaaatcgatt gaacagcacc agcccgttgg
      541 cccccgcgtc ctccaaccga tgtgcgaagt gacagagcga cgtatagaac ggggacaact
      601 tgaccgccag cggcagcccc gtgcgcccgc gcaccgtctt gaccatctct aacgtgcgtt
      661 gctcgattgc ctcggaggtg tccgtggcat tggtggcgag ttgatagaca ttcagttcga
      721 gcgcgtccgc cccggcctgc tcacacaagg tcgcgtactc gagccaaccg tggttcgtca
      781 ccccattgag agacgcgatg accgggatcg agacgtgctt ctttaccttg ctgatgtgct
      841 ccagatactc ctccgaagac agtgcaaact catcgctgga cgggaaatag tcactcgcct
      901 cggcgtgtga gagttcgtgc cttcccatca cctgctcgat gaagccattc tcgagcagga
      961 tctgttcttc gaacaaggag tgcaagacca ccgcggccgc gccagcgtcc tcggccctac
     1021 gtatggtgtc gagctcgtgc ccgagcggtg aagcgccgag cacgaacgga tgggccaaat
     1081 tcaaacccaa gtagcgagtt cccagattca tgacttccct ccgtcgccgt tgctcggcct
     1141 tgactccgtg ggcgcgggcg ccttggattc tttcggcgcc ggcgcgatat gcgagagctc
     1201 ttcgtagacg cggtttcgac gcgcggccat gtgctccgcc gcgactagca gcgcctcgaa
     1261 gcgggctttg tcggtcttct ccaacatccg gaaacgcgcc tcgttgttca tgtactctcg
     1321 aaccttggcc ttgcccggct gctggtcgat caccagtggg cgctcgccct gctcggtgcg
     1381 ctgcggattg taggtgtaga gcggccaaac tccggagtct accgctagct tttgttgctg
     1441 ggcgccaaac tgcaattcgt aaccgtgcgc gatgcagtga ctgtaagcga tgacgaggga
     1501 gggcccgggg tgagcatccg cttcggccag agcccgcact gtctgcgcgt ctttcgcgcc
     1561 gaatgcgatt gaggctacgt atacgtgacc gtagctcatg gcgagtagcc cgagatcctt
     1621 cttaccgact gacttgccca aggttgcgta cttagccacc gcgccgagcg gtgttgcctt
     1681 cgactgctga ccgcccgtat tggaataaac ctctgtatcc aagaccaata cgttgatctt
     1741 ccggttcgag gccaagacgt gatccaggcc tccgaagccg atgtcgtagg cccagccatc
     1801 gccaccgacc aaccacacgc tcttcttgac caggtagtcc gccgcttcca gcaggcgcac
     1861 cgcgtcggga tcttcactgc cactcagcgc ggcccgcaaa cgctcgaccc gcgctcgctg
     1921 cgcgtcgatt tgagcgtcgt tgtcttgcgc ggctgtcaac aactcctgaa ccaactctgg
     1981 atccagctcg cgctgtagct tgcggagcag gctacgcgca gacgaggtca gctgatcgac
     2041 ggacactcgc atgccgaatc caaactcggc gttgtcttcg aacagcgagt tggcccaagc
     2101 cggcccgcgc cctttcccgt cgaccgtata tggggtggtg ggtaagtttc ccccgtagat
     2161 agacgagcag cccgtggcgt tggcgatcaa cagccggtcg ccgaacagct gagtgagcag
     2221 cttgacatac ggtgtttcgc cgcaacctgg gcacgccccg gagaactcga acagggggtc
     2281 gagcagctgc gagctcttca cgtccaagcg cacgcggctg cgtgacatcg ctggcagacc
     2341 aatgaagaat tcgaagttac gagcttcact ttcgcgcagc ggacgttgcg gcgccatctc
     2401 gagtgacttg tggcgcgggt tcgatttgtc cttggccggg caaatctcga cacacaggtg
     2461 gcaaccggtg cagtcttccg gcgcaacctg gatggtgtaa cccttgccgg cgaactcgcc
     2521 ccctttccag gtcatcgact tgaactctgg tggtgcggcc gcgagctccg caggatcgta
     2581 aaccttggcc cggatcgcgg cgtgtgggca taccagcgcg catttattgc actgcacgca
     2641 aatgctcggg tcccatacgg gaatctcggc ggcgatattg cgcttctccc aacgtgcggt
     2701 cgcggtcggc caacacccgt ctactggaaa cgcactgacc gggagtaggt caccgtggcc
     2761 ttcgagaatg gcggccgtaa cccgttgcac gaagtccggc gccttacccg gaatgcgcgg
     2821 cgggcggcgc tgcgcgtgag gcgcttgctc cggcaccttt acctggctga gcccagacac
     2881 actattgtcc agcaccgccc aattgcgccg gacgagctcc ccaccctttt taccgtagct
     2941 ctcctcgatg gcgtgcttca tcgcgcccac ggccagttcg ggcttgatta gattggtcaa
     3001 agcgaagaag caggcctgca tcactgtgtt gatgcgacgc ccaagaccag ctttcgaggc
     3061 aatggcgtag gcgtcgacca cgtacacgtt cagattcttt tcccgaactt gctcccagac
     3121 ctcgagggga acgtgctgga agaactcatc cgctgggaac ggactattca cgagcagagt
     3181 accggagtgt ctggctcggg acaccacatc cagagtttca agaaagtcga attggtgaca
     3241 accaacgaaa tcggcctctc gaatcagata cggccgttga aaggtcgctt cgctgaaacg
     3301 caggtgcgaa atcgtgaccg ctcccgactt cttggagtcg taaacgaagt agccctgcac
     3361 attgagggag ctttcggaag agacgatttt gatcgtgttc ttgttggccc cgaccgtacc
     3421 gtcggaaccc agcccgaaga acaccgattc gaacacgccg tcttcacccc gcggcgcggg
     3481 cgcgggcacg gtcaacgatt cattcgtgac gtcatccacg atgccgacag agaagtgccg
     3541 gcgcggcctg ctggacgcca gattggcgaa cacggcctcc accatctccg gcgtgaattc
     3601 cttggaggat agcccatacc ggccggaaac catgcgcggc atgttgcgct gcgggctctc
     3661 gaacaacgcg gccatcacgt ccagatagag cggctcaccc gcggcgccgg gttccttggt
     3721 acggtccaac accgagatac gctccaccgt cgagggcagc gccgcggcca ggtgccgcgc
     3781 cgagaaggga cggaacagcc gcaccaacaa ggcgcccacg cgttctcccc gagctgcctt
     3841 ctcagcggcc acttgttgcg cgagcaaagc gcccgacccc atggccacga ttacgtcggt
     3901 ggcctccgga tggccaacat actcgaacaa cttgtaacgc cggccagttt gattggcgaa
     3961 gtgatccagg gtgcgctcga cgatgtccgg gcaggccgcg tagtatggat tcaccgcctc
     4021 ccgcgcttgg aagtaggtat ccgggttctg cgcggttccg cgcaggctgg gccgatcggg
     4081 ggtcagcgcg cgccggcggt gctcgagaat cgcgcgctcg tcgatcatcg cgcgcagcac
     4141 gtcatcggat agaagagaga cgcgcgtcaa ctcgtgggag gtccggaagc cgtcgaagaa
     4201 gtgcatgaaa ggcacgcgcg aggtcagggc cgccgcgtgc gcgacacagg ccatatcgtg
     4261 ggcttcctgc ggagatgcgg ccgcgagcat ggcaaacccg gtcgcccgac aagccattac
     4321 atcggagtga tccccaaaga tactgagcgc gtgcgtcgcg agcgtgcgag ccgccacgtg
     4381 caccacgaac ggctgtagct cgcccgcgat tttatagaag ttggggatca tcaggagcag
     4441 gccttgagag gccgtgaacg tcgtggacag agcgcccgat gtcagcgcgc catgcactac
     4501 gcccgcggcg cccgcctcgg actgcatctc ggtcacgctc gggacatccc cccagatatt
     4561 cacacgcccc cgcgccgccc actcgtccgc gaactcgccc atcggcgagg cgggtgtgat
     4621 cgggtagatc gcgattactt cgttcagccg gtaggcgaca tcggcagcgg cttgattacc
     4681 gtcgagtgtt tccaggctgt gactcgtctt caggctatgg gagacctcgg gacccggctt
     4741 accttcgacg gccggccgcg cgctccgttc ctgacccttc aactgtgtga ctacggcgct
     4801 cattgcagag aggtgcgcgt tgcacgctgc gtgcccgtgc aaatcccacc aagatcagaa
     4861 aaacctgcgc gaacgaacac agtctgcgaa catcgtgcgt ccctacggat tggagttcga
     4921 agaactgaga tcgatcgggt tctgaagcaa cgggaccggc gacactggcc agggccggca
     4981 tccgtcacgg cgaagagtcg cattcagcat gcgacgctcg aattgcactg ttcgttcggt
     5041 cataccgagg cggagctagg cccggttttt ttggctcggg actcagcaga ccagttgaga
     5101 taggctgatg cgaccccgcg ccatgtcgag ctcgtttccc gctccgccgt cgttcaagag
     5161 tcgctatcca aagaagctcg tgagcgcggc gcaagcgata cgtaacctgg cttcggggca
     5221 acgcgtactc ctgggttccg gagcagccga acccgtgaca ttggtcaacg ccctggtgga
     5281 gcacggtcag cacctcgccg ataccgaggt tgtccatatt ctaacgttgg gccctgcgcc
     5341 gtacgtcgcc cccgagcacc aggggcgttt ccggcatacg gcattcttca tcggccccaa
     5401 tacgcgcgag gcgattcagc aggggcgcgc cgacttcatg ccggtgttct tgtccgagat
     5461 tcccaatcta attcgctcac gtcgcattcg aatcgacgcg gtgctgattc aggtcgcgcc
     5521 cccggatcgg cacgggtacg cgagtctcgg tgtgtccgta gacgtggtgc gctcggcggt
     5581 agacgccgca gagctcgtga tcgcagaggt gaaccccaac atgccgcgca cgctggggga
     5641 ctctttcgtg cacgtcaatc gtatcgacta cctgatcgaa gtcgacacgc cactgctcga
     5701 gctccttccc gaaccgctcg acgacgtcgc cgaagaaatc ggcaagaacg tcgcgacgct
     5761 gattccagac ggcgctaccc tgcaaaccgg cattgggcgt attccccacg cggtgttgaa
     5821 cgcgctgcaa gggcatcacg atctcggcct ccacaccgaa atgctatctg acacggtgat
     5881 cgagctcgtc gaggcggggg tcatcaacgg tcggcgcaag aatctgtgtc ccggtaaatt
     5941 ggtcacttcg ttcgtgatgg gaacccaacg cctctacgag tgggtggatg acaacccctt
     6001 gatcgagatg cgcccaaccg agttcgtaaa cgaccctacg acgattgcgc gcaacgacaa
     6061 catgatctcg atcaactcgg cgctagcggt ggatctcacc ggccaagtcg cggccgacac
     6121 cctgcacggc cgcttcttct cgggcatagg cgggcaggtc gacttcatcc gtggcgccgc
     6181 ccggagccgc ggaggcaaac ccatcattgc actacccgcg tgcgccgaga acgggaccat
     6241 cagtcgtatc caagccgcgc tcgagccggg cgcgggcgtg gtaacgagcc gcggtgacgt
     6301 gcactacgtg gtgacggaat acggggtcgc ggatctgtgg ggcaagaccg ttcgcgaacg
     6361 ggccatgtcc ttgatcgcga ttgcgcaccc ggatttccgc cccgatcttc tgaatcaagc
     6421 caagcaaagg cactacgtct ttccggatca gtgcgtgccg catggcccct accccggcgg
     6481 cacgactcgc gtggtgaccc tacgcgatcg cagagatgtc gtgattcggc cgattcggat
     6541 caccgacgag cgagcattgc aggagctgtt ttatcggctc tccagcgaga gcgtctacca
     6601 acgcttcttg cacttcaaaa acaagcaccc ccatcaggag atgctggccc tagtggatca
     6661 cgactacgat agcgcggtgg cgttggtcgc ggcgcccgtg gacgatgata cgggtcggct
     6721 cgttgcgatg gcccgttacg atgtggatcc ggcgactcac ttcgccgacg ttgctttcgt
     6781 cgtggacgat cagtggcagc gggtgggctt gggtaccctc ttgttctccc gcctcggcga
     6841 ggtcgcgaag gccaaaggct tgcgcggatt tacggccgac gtgttgtcct cgaacaaggc
     6901 catgctcggt gtatttcaag cgagcgggat gcgctgtgaa accgagcggg acggcaatgt
     6961 cacccacctg acgatgcggt tttcgcaatc cgccgtgtga gccgcctcag cgattgacgc
     7021 gattgatcgg cttgccctgc aatatgccgc gcacattctc gaccgtcgtg cgcagcagcc
     7081 gcttgcgcgc cgccaggctg gtccaagcca agtgcggcgt gatgatgcaa ttctgcagct
     7141 gagtcagcgg gtgatccggt ggcgggggtt cttgactcag cacatccagg gctgcccccg
     7201 cgatggtctg gtctcgcagc gcgtcggcca agtcgagctc ctgcacgagc gcgcctcgcg
     7261 acgtattgat gaggaacgcg gagcgcttca tgctccgcag gcgcgcctca tcgaccaggc
     7321 cgcgcgtctg ttcggtaagc gggcaatgca aagacaccac gtccgactgc tcgaacacgg
     7381 tatccaggtt cgcggtcgtc acgaagctct ccggcgggcg ctgccgcgac ggcgtactca
     7441 gcaccaccat cccgaaagcc tgcgccaccc gagccaccgc gcttccgatc gagccgtacc
     7501 cgacgacgcc gaagcgcaaa ccgtgcaatt cgatctgagg tgtccgccaa aacgcgaaat
     7561 ccggactgct cgcccaatgc ccttcgtgca ccaggcgatc gtgcaaccca acccttcggc
     7621 agagttcgaa caacagcgcg aaggtatgct cgaccacact cgccgtgctg tacgcgggca
     7681 cgttacacac ggcaacgccg ccgcgcgagg cggcctccaa atccacgatg ttgtatcccg
     7741 tggccgagac gcaaatcccg cgcagttcgg gcaacgcctc gagcgtcgcc gcgtcgaaag
     7801 gcactttgtt cgtgatcacg acttggcacc ctcggctgcg ttcgatcact aggtttggcg
     7861 gggttcgctc gtagacgctc agctccgcga gctgctcgag ctccgaccaa ctgttgtcac
     7921 ccggatttac ggtgtaccca tcgagcacca cgacgcgcgg catgctcgtt cttaccacgc
     7981 agccagcggc ctgcggctgc atatcgccgt caggccgagc tccactagct ctgcggttcc
     8041 aaccctgaat ttcgcgccgg ctcgtccatc gcgggcgcga aactcgagct caccggcgag
     8101 cttcgttgat cgcagccaag ctcaggaact gctggaacgc gcggcgttgt gcctcgaccg
     8161 gcacgcgctc gtatgaatag gagtggggca cgtggttggc aaataggcac tgcacggcct
     8221 tgtcgaagct acgcgcggcc ggagcgacat caccagcctt ggcgctgtct tcggccactc
     8281 tccgtaccgc cgtctcgact tgttcgcacc agcccgacgt cttgggtaag aagggtgggg
     8341 gcgcagcttc acaacagctc ttgcgaacga tggccgccgc ggggagctcg taccagccga
     8401 agcccgctcc gcccagatcc ttgcgctcgc tccgagcgcc cgcgaagaga cgccgggccg
     8461 tttccctgaa gtcgccttct tcgcagagga acgcgaagtt cgcctcacca cgcggcagca
     8521 cttccggcag gtaggcagtt acgcaggtat cccgccgcgc ttcggcggat accttcggtt
     8581 tcgcactcgc cgatgcattg gcgggcgcct ctgcgcgcgg gacggtggcc gtattcgcgg
     8641 ctgcggccgg cgcacccgcc gagcgagtga gactcaacaa cagatagacg caaaagccac
     8701 caccagccgc gccgccaacc accaacggcc acactcggcg cttcttctcg aaagggttga
     8761 agctcagccg cttcgagatg tgcgccggtc gcaccgttcc caaaggcggc tgggggagcg
     8821 cgggcaggag ctgcgccgaa agctcttcga gagttgggcc ggagctgtgc gggcgagagg
     8881 gctgagaact cgcactcggc aacggaggag gaaccggtgc ggcggtgacc ggcagcggtg
     8941 gaggagtggg tgcggccgac actggcagcg gaggaggaat cgggacggaa actgcgggca
     9001 tcggcagcgg cggcatactc cgtcgcggac gcggaacgag ttgaactgca ccttcgtcgt
     9061 gcggcagcgt ggaaccgtcc actaccaaga catcgtgttc gggtgggacc cgctggctgt
     9121 ccgcctcgag ggcacgaggg cgtaccgccg gcgcgcgccg cgggggcatg gccttgggat
     9181 tgcgctccca ggcgtcgaga catcgaatca gctccgccag ctcggtcatg cggagccgct
     9241 tttcgccatt caacccgcga atcaagattt cgttcagggc tggttcctgt actccatgtg
     9301 tggagagcgg cgcgagcctg ccaaccagca tctgacgggt gagtagcgcc cggtctgccc
     9361 cgacgaaagg tggcacccca gtcagagccg tatagagcag agcgtgcagc gcccaaacgt
     9421 ctgacgattc ttggtccgcg gctcccttca aacgctccgg cggaaggtac gcaccaatca
     9481 cgggtgccac gaacatcgaa agcaccggcg cgatgctgcg cccgtccggc accgcgatca
     9541 cggaccacgg agaaatggct ccgtggactg caccctgtgc gtgcagcact tggaccgcct
     9601 cggacagacg tagcacccag gcaacggcct tgtcgggtgg cagaggtttg ccagcctgaa
     9661 actgatgaag tgtctgacca ggaacgtatt cggcaacggc caccgaaatt ccgggctcga
     9721 aacccatgcc ttctggaaca gcgctcgctt cgacgtggcg aatgacttga agcacactcg
     9781 ccaaatgcgg atgattggca ccctgggccg cttctacgtg cttgagacgg tgttcaggga
     9841 tcagcgaagc aacgaattgt tgcccttgcg ccgcgtcttg ggcaagccaa cgcggcaaag
     9901 cttgctgtga tttgagaaga ccgtggaacg catagcgtcc accaatgggc gttggacgat
     9961 tcgacatcct tacacctagc taccttacat tcagccacac cactcgatcg gcggacactc
    10021 gccgacgcga gcatcgccgc tcgaacggca tcgagtctga aacacgaacg cgcgaagtca
    10081 gactggagaa tagcactctc aatacccaac ggttgaattg attcacgagg tgagtacgga
    10141 tttcgtttct tttgacggat taccgacgca gctcttgtgt tcacgtctgc gctggttact
    10201 cagatcagcc cgctactctt tttagagagg tactctttgt agagaggtac tcgttgccca
    10261 gagctacgct ggtcagaccg ccaatcctcg gggcgccacc ccgacctcgc tgatgttcga
    10321 gttcgaggag caggctttgg cttcgcgctt cggttgctcg gttcgggctg atactcggct
    10381 actctcgtct acattggctt cgatggttca acttggacgc agatcggaca tgttgaccca
    10441 accgtgtcga tcccaaccgt gtcgatccca accgtgtcga cctcaaatat gtcgatgcca
    10501 accgtgtcga tccgctgcat ttcggtgctg cattcccgca acctatcggt tgcgacccgt
    10561 actccgggcc gcaatgcgct cccgcaacca atgggttgca tttactcact gcgcaaccga
    10621 aaggttgcac tatgcatcat gtggaccgag cgtttgtggc gggttttggg tcgaccgcgg
    10681 gtcagcggcg gggagcgctc tggtatactt cgcggcgcgg tcgcggttca acaccgcgtg
    10741 aaaccgcgga cagttcggcg tacccatcgc gcatcgcacc actcgaatcg gatttgagat
    10801 gaaaacttcg cttcagcggc tgactcgtaa gattccgagg ctaccgagta gcctcgcgtc
    10861 ggagttgggt cgcttgccgc tcgcattcgc gctgcggcaa gtcttgacca cgggatatgg
    10921 ggtgaacgca ttcaaagccg atgtgttggc gggcgcagtc gtcgcgatcg tcgcgctgcc
    10981 gctgtccatg gcgctcgcga tcgcggtggg cgtaccacca cagcatggtt tgtacacggc
    11041 tatcgtggcg ggcgggagcg tggcgctgct cggagggtcc cgatttcaag tcacgggtcc
    11101 cacggcagca ttcatcgtgg tgctcgcgcc aatcgtgggt aagtacgggt tgcccggatt
    11161 gttgacggca ggcttgctgt cggggctcat tctcgtactc atggggttcg cccggctcgg
    11221 gcgctggatt gagtacattc cgcatcccgt gacgaccggc ttcacggcgg ggatcgcaac
    11281 cgtcattttc atcctgcagt tgaaggatgc gctcggactc acgacgggcg cccttcccga
    11341 tcacttcccc gaaaaggtgg tcgcgctgtg gcacgcgcga ggcacggcgt cgttgccctc
    11401 ggtgtgtgtc gctgccttca cgcttctggt actgctgggc tttccgcgac tcaccaagcg
    11461 cgtgcctgct ccgctagtgg caatcgtcag cagctcgatc ttggtggcag tgttggacgc
    11521 catcgcccca aacttggata tcgcgaccgt gggctcgcgt tttcacactg agttgaacgg
    11581 caagctcatc ccaggcattc cgcgctctgg cccgacgctc gcagccccct gggccccgca
    11641 cgcgctgaac ttggagttcg tccgagattt gctgccgtcc gcattcgcga tcgccatgct
    11701 cggtgcgatc gagtcgttgc tgagcgcggt catcgctgac gggatgacag gcactcggca
    11761 tgagccgaac tcggagctca cggcgctcgg gatcggcaat atactcactc cactgtttgg
    11821 tgggattccg gccacgggag ccctggcgcg tacggccacc aatgtacgtt cgggagcaaa
    11881 gagcccgatt gccgccttca cgcacgccgt gctcgtattg ctggcgatct taatcgcagc
    11941 gccttgggtg gcctacgtac cgatggcatc tttggcggca ctcttgatgt tggtcgcatg
    12001 gaacatgtcg gaggtgcgcc acgcctggca cgtcgtccgg gttgctcctc gcagcgacgt
    12061 gacagttctc atcacttgct acgcgctgac cgtcttcttc gatatggtga tcgcaatcac
    12121 ctttggggtt ctgcttgcag cagtgctttt catgcggcgc atggccgagc tcacccaaac
    12181 gcgagtgctt cacggctcga gcgagggaaa ctcggtgcga gataccgagc agttgccggc
    12241 cgcgattgca gtttacgaaa ttctggggcc actcttcttc ggggcagcgc agcgcggcat
    12301 ggcggcgctg gatgcactct cggaccgagt caggattgtg gtcctcgacc tgagtcgcgt
    12361 ccctgtcatc gatgcctcgg gcttggtagc cttggagagt gcgctcgaac gcctgagaag
    12421 agctcgacgt ttcgtaattc tgggcggacc tctgccggaa ccgaaagcgg tattcgatcg
    12481 agccgagctc gaaaagcacc atgagaatat ccttctgacc gagtcacccg gcgaagcggt
    12541 ggccatggct aaaacgctgg tcctcctgaa tccagagtgg gacgttgccg ccaaaaccca
    12601 tctgggtggg ctcagcgaac gaccgcagcg agtcttgtga attagtactc caaccgctgc
    12661 cgtttacacg gttgaggaac ttccaagcag gaaaccggcg gccctgccga gcggtgcgcg
    12721 tctccgcgcc tcgcgacaga cagaaggact ggtgttgagg aattctgggc tgtagtagaa
    12781 aaagaggcca cgctgacatg gagcgtatgg cccgatggcc aacacagggg atggatgacg
    12841 cgaattccgc cgtgatatag gctgaaggcg tggaggcctt cccgtcgtga ccgacttccc
    12901 cgccctagta cgcgatgtaa tgaccaagga tgtagtgact ttggatcgca acgataagtt
    12961 gttggtcgca gacgatctca tgcggctagg acgaattcga caccttcccg tagtcgacga
    13021 gggcggtaac ctcgcgggaa tcgtcagtca acgagacctg ttccacagcg ggctgatcaa
    13081 ggcactcgga tacggcgcgc acgcgcaaag acaggcgctc gacatggtcg tagtgaagga
    13141 agccatgcga tcggacgtgg tgacaacgac gccggagacc cttctccaag aagctgcgag
    13201 cgtgatgttg gagagaaaga taggctgcct ggttgtcttg gaaggcggca ggatagctgg
    13261 aattctcacc gagagtgact tcgtcaagta cgtctcccgc aagtaagtac cctatcgagg
    13321 gctgggccgc cgacgctcat tgcttctaac cgcccatgcc aatgaggagc gcggcgtgag
    13381 gagcgcggcg agggtcgccg aggtcggagg ggctctgttt caatctctag ccgagcatca
    13441 attttgctca cgggctcctc actttggcta gctgcagtgt tggttcgtta ctactggtcg
    13501 cgtcggacca ggcccacgcg aagccaaggt cgatgaggac atgacggcag ggggtttacg
    13561 gcccgaggga ataacggcat gagcttcggg ttctccgcag tgcgccaaga tccacttctc
    13621 gaacccatgt gccgaaatcg tcactcagga ccgaaagaac cgttgcggtc gaagggtgtc
    13681 tctgatgaca ccttgtattc tgaatgcggc aactcgctcc cacctctgcg ccagcccggt
    13741 actgaagaac ccggtactga agaacccggt gccctaagaa ggcctgcgcg gaggcggagt
    13801 tccaaaagag accaattgcg tgtccccaag ctactcctta ggacgccaac gcgcctgcgt
    13861 actggtgggc tgccgacagg aggagcgggc gccaagcgcg ttcgagcccc aaggcgagcg
    13921 atgccttttg gctcgagatg gggaaaggca ccctaccgca ggaagagaac tagcgtacaa
    13981 acccgtgcca cgagccgact gggattctga gggcccactg ccgtggcact cccccactgc
    14041 cgcaatgggt tcctcgtccg gtatcctcgg atcccgcgag cacgtttcca ctctgactcc
    14101 ccggcggcgc gggatgcgac tacaggtgcc aaatccttgc cacgaccggg ctgagccttg
    14161 aattgggcga tggcgagatg ggctaggggc gtttggccaa acgcgcttgt ccctcacgtt
    14221 tctacccggc agacacgggc ggagtgagga actactactt ggaccctgtc tagaatcggg
    14281 tgtcgtattc ggctaagaac ctatcccagt aggagcgcca gagacggtgg ttgtccgagc
    14341 acggcggcgc tcgagctggc ggggcggcgc gcgcaatcgg atgctggcga ctagctccta
    14401 tcgaaccggg gaagccagcg aggcccgcac gtagcccgac cgcgcgagac tcccctgatg
    14461 gaactggact agtacgacgc gacatcgcct gaccgtcatg ggcggaaggt caggctggac
    14521 gaataacgct cgagtgaact ctcgtggccg actctacggg gcgggatctg acattcggat
    14581 tgagcgcagg ttagacacct aggagccgga ttctggactt cacggcacgc gactacgtca
    14641 cagagcagtt cagtaaagct tttgaatctg ccccggagct ctggacaatc tacaagtcat
    14701 caaaaagcgc ttcagcaatc tgaaactggg atggcggatt cggaaaccgg gattatgttt
    14761 ctcactccca tccagttcgc tccaaaggca cacatcgaaa cgcacttgcg aattgctgat
    14821 tggagttgac agtttccact taaacgcata cgaagcgccc ctatggctag tcccgtatct
    14881 gaccaagttc gtgctcgtgt tgaatcgttc gtggcagagc tgagcgagtt aattcgccgc
    14941 tccgctatgg agacggtgca gaatgcactg tcgggtgaca gtgacagtcc gcgacgcggc
    15001 cgtcgtcccg cggctgctgc cgctgcggcg ccggcacccg cccgccgcgc tgccagcgtg
    15061 gctcctggcc ccaagccccg cgccgcaggt ggtcccgctc gcgccaaggg cgccaagcgc
    15121 tccccggagg agctcgagga gctgacgact cagctgctcg agtacatcaa gaacaacccc
    15181 ggtcagcgca tcgagcagat cgccgaaggc ctcggtacct ctacgaagga gctgaaccta
    15241 cccgcgaaga agctgctgaa caccaaggcg atcaagacca agggtcacaa gcgcgccact
    15301 cagtatctgc cgcgttgaag tcaggctgac cagctctgcc attggcgaat accgaacaca
    15361 ggttggcaat tccgaccttt acccctcagc cgaaggacgc ttggggctcg ggagcctttg
    15421 aagagccgtc agtgtccacg cggcaaagcg cggtcggttc tctgtagagc tattgtacct
    15481 atagagctat acggacgcgc aatccgtgcg gaccgcagcg cggtcattcg tggaatactg
    15541 gagaactagg ttgatattcc ccctgcgcgg caaggggagc caccgggcac tagcacggca
    15601 agtatggaat ctcgatgatt ccctcgagcc gctgtgtcag tcgacggttg tcagtccagt
    15661 cctattggca gtacacgtct ggagcgctgt atcccggtcg ctttcgaaag ttggttacgc
    15721 cctcagtgcg tatctcgaag cggccggggc ccggggggcc gactggcagc gcccgggttc
    15781 tggatccgag cgagacgatg aaagctccgg ccgctcgagc cgattctctg tcatccggaa
    15841 agctgctccg cgggcaccgc gtaatgtgcg ggcctcggat tgaggcctag aggcagcaga
    15901 gctttgctgc gcctgtcgat cgacagtcta actgtcctgt ctttggctca cgtccatcgt
    15961 agtttcgtcg cccatggggg ctaactgccc catggcatgt ttccgcgctg agaatatgga
    16021 ctcgtcgccg tgaagcgtac atgctccggc gcgcgtgact tgcggaatac ttgaccggtt
    16081 caaggcagcg aacttcggac agcgctccag cggacgctca caatccgtgg tctcccccac
    16141 cgcttccgac ccccgtggtt cagactctgg tcgcaagcgc tcttcgcggg cgcgctcgcg
    16201 gttgaacacc gtggctgcgt ttggcctcat tctcttgtgt cagagtctgg ccgtcgccgc
    16261 ggagccggtg agtcgcagcc acgtcgttta cgatggccaa cgtctcggca gcattgccaa
    16321 gcgatatggg gtcagcgtcg aggccatttg cggcgccaac gatatgcgcc cgacggatcg
    16381 gctgaagccg ggtcagcgtc tcgtgattcc gaatcgaggt gaccgcgacg gcagccgggc
    16441 tcgcgccgcc aactcgtcaa tgactcgccg acggagcgcc accgaaccgc aggatggcgg
    16501 cccggcgact catcgagtcg agagcggcca gcgactggag tcgattgctc gccgctacgg
    16561 agtcaaggtc gagg
//
LOCUS       DASTKU010000902         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_399172, whole genome shotgun sequence.
ACCESSION   DASTKU010000902 DASTKU010000000
VERSION     DASTKU010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399172"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2513)
                     /locus_tag="VFQ61_31620"
     CDS             complement(<1..2513)
                     /locus_tag="VFQ61_31620"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET9959097.1"
                     /translation="MNSTLPASAAPEVEQWLCSRLASALDLELEAVRSAVSFSALGLD
                     SMAISKLARELSARFGIRVSVSVLFAHPSVLELTRALTGLTRALPGQGSAALQPEESR
                     LGRTGEVHEKIAVVSLACRFPGAPDPESFWRLLLEGRHAIVELPEGRFSRGVVDSDPR
                     KPGKTHVAHGGFLNRVDAFDPLFFGISPREARQMDPQQRLMLEVCWEALERAGLRPSS
                     LRESRTGVFLGAIWADYARLAGARLEGLELHSATGQALNLIANRISYAFGLRGPSLVV
                     DTACSSSLVALHLAARAIRSGECDAALAGGVNLILSEANLVALAKFGGLTTDARCKAF
                     DARADGFVRGEGAGVVMLKRASLALRDGDPVLCFIAGSAVNNDGPSNGLTAPNPRAQV
                     EVLRAACEDAGLDPARVGYVEAHGTGTELGDPIEASAIGEVYGRSERSRPVRVGSVKT
                     NIGHLEGAAGIAGFAKAVLAVSQRVLPASLHFEQPNPHIPFDELGVQVQIRTEPWVNE
                     GGPRYAAVSSFGWGGTNCHVILQQNLTAPEDELAGENRPLEHSSADRAPAVSSLEAEA
                     AGASDVAESVNDWVFVCSPQGGQWARMGQSLLRDVPAFRARAQRFDEAYRKVSGQSLL
                     TALASGRAGSPRARVDVLQPLIVLVQLGLAAAFEAAGIEPSAVIGHSLGEVSAARLAG
                     GLDDDDTALLLHHYSRLQATTDGEGTMATVATDMGGLAPLLEHYGPDLTWAGSNSASS
                     SLLSGKRAAVADAIARLEEQGIFARPIAVNVAAHSAAMDPILAELHAKIASIRARALA
                     VPMFSSLTTEWVSSGELDASYWPKNLRLPVRFGQAFEKL"
BASE COUNT          524 a          921 c          745 g          394 t
ORIGIN      
        1 atcaacttct cgaacgcctg cccgaagcgc acgggcaatc gcaaattctt cggccagtag
       61 ctcgcgtcca gctccccgga gctcacccac tccgtggtca aggaggagaa catcggcacc
      121 gccaaagctc gagcccgaat cgaagcaatc ttggcgtgca gctcggccag gatcggatcc
      181 attgccgctg agtgcgccgc aacattgacg gcgatgggtc gcgcaaagat accctgctcc
      241 tccagccgag cgatcgcgtc cgcaaccgcc gcacgcttac cggacagcaa tgaagaagac
      301 gccgaattcg agcccgccca cgtcagatcc ggtccgtagt gctcgagcaa aggcgcgagc
      361 ccgcccatgt cggtcgctac ggtcgccatg gtcccctcac catccgtcgt cgcctgcaga
      421 cgagagtaat ggtgcaggag caaggccgta tcgtcgtcat cgagcccgcc ggcgagacga
      481 gctgcactca cctcccccaa gctgtgacca atgacggcgc tcggctcgat gcccgcggcc
      541 tcgaaggctg cggccagccc caattggacg agcacaatca agggctgcag tacatccacg
      601 cgcgctctcg gcgagcccgc gcggccgctg gccagagccg tcaagagcga ctggccggag
      661 accttacgat acgcttcgtc gaagcgttgc gcgcgagcac gaaaagccgg cacatcccgc
      721 aagagtgact gacccatgcg cgcccattgc ccaccctgag gcgagcagac gaaaacccaa
      781 tcgttcaccg actcggccac atccgatgca cctgccgcct ccgcctcgag tgagctcacg
      841 gccggtgccc gatccgcaga actgtgttcg agaggcctat tctcgcccgc caactcgtct
      901 tcgggggcag tcaaattctg ctgcaggatg acgtgacaat tggtacctcc ccatccgaac
      961 gagctcacgg ccgcataccg aggtccgccc tcgttgaccc agggctcggt tcgaatctga
     1021 acctgaacac ccagttcatc gaaagggata tgcggattgg gttgctcgaa atgtaagctc
     1081 gccgggagca ccctctgact caccgccagc acggccttcg cgaagccagc gatccccgcc
     1141 gccccttcta ggtgaccgat gttggtcttc acagatccca cccgtacggg ccgggatcgt
     1201 tccgatcggc cgtagacctc tccgatggcg ctcgcctcga tcggatcgcc gagctccgtc
     1261 ccagtcccgt gcgcctcgac gtagcccacc cgagccggat ccagccctgc gtcctcgcag
     1321 gccgcgcgaa gcacctcgac ttgcgcacgc ggattcgggg cggtgaggcc gttactagga
     1381 ccgtcgttgt tcacggcgct accagcaatg aaacagagaa cgggatcccc gtcgcgcagc
     1441 gcgagcgagg cgcgcttgag catcacgacc ccggcgcctt caccgcgcac gaagccatcc
     1501 gcgcgcgcgt cgaacgcctt acagcgagca tcggtggtca agccgccgaa cttggccagc
     1561 gcgaccaggt tggcctcact caagattaga ttcaccccac cggccaacgc ggcgtcgcac
     1621 tcacctgacc ggatcgctct tgcggccaag tgcagcgcta ccagggagga cgagcaggcg
     1681 gtgtccacga ccaggctcgg cccacgcagc ccgaacgcgt aggagatacg gttcgcgatc
     1741 aaattgagcg cttgccccgt cgccgagtgc agctcgaggc cttcgagtcg tgcgcccgcg
     1801 agacgcgcgt agtcggccca aatcgcgccc aaaaacacac ctgtgcgcga ttcacgcagc
     1861 gaagaggggc gtagcccagc gcgctcgagc gcctcccaac agacctcgag catcaaccgc
     1921 tgctgtgggt ccatctggcg cgcttcgcgc ggtgagatcc cgaaaaacag gggatcgaag
     1981 gcgtccactc gattcaagaa tccgccgtgt gcaacgtgag tcttgcccgg cttccgagga
     2041 tccgagtcca cgaccccacg cgaaaagcgc ccctccggaa gctcgacaat cgcgtgacga
     2101 ccttccaaaa gcagacgcca aaacgactcg gggtccggcg caccgggaaa gcgacacgcc
     2161 aagctcacga ccgcgatctt ctcgtgcact tctccagttc ggccgagccg ggactcttcc
     2221 ggctgcaatg ccgctgagcc ctgccctggg agggcacgag tgagccccgt gagggcacga
     2281 gtgagctcta aaacgctcgg atgggcaaac agcacgctca ccgaaacgcg tatcccgaat
     2341 cgcgcgctca actctcgagc cagcttcgaa atcgccatcg agtccagccc gagcgcgctg
     2401 aagctaaccg cgctacgcac ggcctcgagc tccaggtcca gcgcactcgc cagccgagag
     2461 cacagccact gttcgacttc cggtgccgca ctggcaggca acgtagaatt catgtccgca
     2521 ccttcaagac ccgaactgca cgacttccag caccgtcggc ttgatgcgcc gcttcaacgg
     2581 caag
//
LOCUS       DASTKU010000903         9702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_623037, whole genome shotgun sequence.
ACCESSION   DASTKU010000903 DASTKU010000000
VERSION     DASTKU010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9702
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623037"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFQ61_31625"
     CDS             <1..245
                     /locus_tag="VFQ61_31625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959098.1"
                     /translation="CVPESVIGPVWTPTVHALAIFHAISELELRMLLGGACAGSAMLY
                     PGPLGRTTIRAAESRSYNSRAPKTFGIRHHLTEAEK"
     gene            624..5243
                     /locus_tag="VFQ61_31630"
     CDS             624..5243
                     /locus_tag="VFQ61_31630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959099.1"
                     /translation="MMRQGWLLAAISGVFLVGCGAEESGSSKLAKNKEVASSRVSQSL
                     TSNLETETRGAAPDAGCALLDNPELRPRMSAALENKLLVLCGRAQKSAQVTTVPGGLP
                     TRARRAAAALSADIPVSDPALDTGGSTQSETSVVAVGNIVCAAWNDAGEGAGLNGFAG
                     FGFSLDGGLTFKDGGPFPIGPGGDQSFGDPSLAYSARDKAFYFASLSSQGLSLWRSLN
                     GCQSFEYVGAIQSGFGHDKELIAVDNDPGSPYFGRIHMGWTAFGQSNDLNVTSYSDDA
                     GKTWAPVAHLPNSGGAGQGVYPAVAPNGDVYMALVNRAFNIGGVQDQWIYGSKDGGQT
                     WSKLADIGTAQLQPEDANATQDCGRQALNGHIRNLSSPQIVATPDSSASAGYVIHAIY
                     PYDSDGAGPDHSNVFYRRSSDGASTWSAEVQLNDDGTNTDQFYPALGVDESGVLAASW
                     YDRRLDPLGNTSFDRYLTVSADGGLSWSTNERVSDVSSPVAQTLPNFDGLASCYHGDY
                     DQIAVSGGVAHLIWSDDRRVTELGPNPDVYYDQFAINVHLGKIRAQQSGVSCSSRLSF
                     SLTDQDLAGSGAHEITVTTTGGDSETLVLTEEGSRPGAFSGAIATTSGPLQLGNGSLQ
                     VEDGATITATYLDADDGKGNPAVSTAEVRVDCAPPVLQNARVVALTGTGATITVDSSE
                     SAALTIQYGFACADLTQSATSSVAAIPTVALDGLYSGMTYFYALTATDAFGNSVTDDN
                     DGSCYSFKTLDVLYSVNFEAGLAGFEIDNGSTNTGGSGGTGGFGGKPSFSGSLGAGGI
                     PSVSGAPGQGGSSGSGKGGAPGTGGTSSWEEGGVGGAVEEGGSVGTGGSSGSSGSTSR
                     NGLWHLSQGCAAALVGHSRPFALYYGQDSTCTFDTGTINEGFATSPPILLSDASFATV
                     EFNYFLGTEGGGFYDQASLEVSVNQGPFQVVASNFTSLLSAVKAIRKRADARPAGRYS
                     LVSNRGEWEHATADLTPFLQGLTQAEIRLRFHFNSVDSFANAFAGFYVDDIKVLGVQA
                     PIPCRQNADCDDGLFCTGNETCSAGFCAKGLPVVCTGPEDGVACTQLTCDETARGCIE
                     KPIDELCDDGTFCNGPERCDVTLGCQPGAPVVCGASKVDCVISQCEERVKNCVQFPDH
                     ARCDDGSFCTGQEFCDISTGCVAGSPPCQDPVSCTDDLCDEATFSCNFVPNDTYCNDG
                     LFCNGAEYCDGYQGCRTTGSPCSASDKCDEKGDQCVPICFTDVNSNHQTAGRAYAKKK
                     AFYALGSGDALGTASEVTSLQGSGTFWKHVPSCPAPPSIDSVQVSVSGSVARVSGVAS
                     DPNGDIVKVRLTFWLHGSLQIVLDAKGTTNWSGDLGLPEGTHAVSAQAVDRAGFVSAW
                     SDLYFFTVLPALPPVLKSVAVSVKGSNAIVSGSAGDPNNDIQYVEVTILQNNEIVAFR
                     KAQGTSDFSAIILGLAAGKYSARAQAFDSTGFASALTDPVTFDIAAEQCVTDSNGGHK
                     SFGRAKSAQGNYYAVGSGDALGHKASTITALSGSAEFWTRVARCP"
     gene            5352..6158
                     /locus_tag="VFQ61_31635"
     CDS             5352..6158
                     /locus_tag="VFQ61_31635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959100.1"
                     /translation="MRPAPLVRIPNVVTLATLTACLGVLAPACESDDSSSAPTGGLHS
                     GGGTVRSGGTSHSVAGASSGAGVNSGAGRQDAGHGGDSGGASTGGNTEAEGGNAETQG
                     GSNANTSNGGNPQSTRGGANNTQSSGASASGGAAGADSIGGAAGDVNSGGLTNSGGTL
                     GGGMTSVVSGGSSNTSGGTANTSGGASGGTSNSAGATSAPNCEALSCVSGSSCQCCPR
                     GGPTQICICSTACQGDSDCLDPSTPSCSKAPGAASGFCTAELKLCCWQCE"
     gene            6464..7015
                     /locus_tag="VFQ61_31640"
     CDS             6464..7015
                     /locus_tag="VFQ61_31640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959101.1"
                     /translation="MAKGNHVFNHFEKTIRNAFEAFLTEGGVQVEDRAVDVNSAIAMP
                     GSADAEAIRARYPRAWAKFKEIVSAPQHKEMEGMQDDSPVAANDPNSQSYGIAAAQFI
                     SGVRALASAQPGMPPGAAWQAMVKSGFDAAAADRYVNAAPRAGQAKRKADIERIRTFI
                     KGRLNSDVSVWIQFMKLDHAGEK"
     gene            7544..8731
                     /locus_tag="VFQ61_31645"
     CDS             7544..8731
                     /locus_tag="VFQ61_31645"
                     /inference="COORDINATES: protein motif:HMM:NF021430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HET9959102.1"
                     /translation="MSSCFVPGSHRASRFLPRRALLAATLFLGLLEQVSWAAPTVDDT
                     RAPVRSRAAPKLSVQAVEREPARKRPSAEELATLRRGVEENPALRKARFELSHALLRA
                     GDLQAAHREAEAWREHDAYNLVVVRMLGDIESELGDRERARRTYSAIVELLPKDVEAR
                     RALSTVLKQAGDLQAARAELVAALALAPNDARTQFELGDVEQRLGMQDSARTRFETTK
                     DAADAPESLRYPARQRLAQIHAQKRLEAGRRGDTNQVHALGQAIDALQIHGGTENDIK
                     VYLSWDSDRTDVDLWVLTPSGEKVFYAQRQGKGGEALFDDVTTGYGPESFTAPHAEPG
                     TYRVQVNYFGSRASTFKEARGEVTVILNEGRATEQRSVFPYRLFEEKDTLTVADIRVE
                     AKP"
     gene            8776..>9702
                     /locus_tag="VFQ61_31650"
     CDS             8776..>9702
                     /locus_tag="VFQ61_31650"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HET9959103.1"
                     /translation="MNRARFGTLALLILLALQGCSNGHVGSLGPHAAASAGSPLIDDE
                     PDPADAGGIGWLTARNERGEGGLIELAQVNIDAEQIGDYAAVSIEHRFVSRATTVQEG
                     TFRFPLPEDALLTGLSMLIDGRVVHGELLEREKARRIYEEIVDSMQDPALLEWEHGST
                     FKMRVFPIEPQQPKLVTIRYLVPLKHKGKQWVFSQGTRQNAGAGKLSELTLRWQGKQV
                     VSERGVQAGRILEATASDPSDVLREERPEAAYTAVRVRPDWAQVPVQTRTAPKHWVFV
                     VDTSRSALEERKLQLETLRTLLAALGPQERFTP"
BASE COUNT         1837 a         2820 c         3194 g         1851 t
ORIGIN      
        1 ggtgtgtccc ggagtccgtg atcgggccgg tatggactcc caccgtccat gctcttgcga
       61 tcttccatgc aatctccgag ctcgagctgc ggatgttatt gggtggggcc tgcgcgggga
      121 gcgcgatgct ctaccccgga ccgcttgggc gaacgacgat acgagccgcc gagtcgcgct
      181 cgtacaacag tcgggcccca aagacatttg gtattcggca ccatctcacc gaggctgaga
      241 agtgaaatcc attggatggg tagaagttga gggacatccg cgccgcgccg tgagtttctg
      301 gcgcgaccgg tttctggccc gaccatccga atttgcgaag ttgcttccga ttcgcagctg
      361 gagaggtatc ggcgtgaagg atgtccggag gtgccgcgga gtattcgacg aaatgacttg
      421 tccgatatcg gagctgattt ccgtcaatgc ttcgacgaat acgatatcgc acgttcgagc
      481 gcgtgccgcc gacaccagga ggtcgccata gtacgaatca cggaacgacc gatcccaagt
      541 gctacagagt gacaatcgag acgctctctg atagtgattt gtccgacgag gaggagcgtc
      601 ggatggcgct cagggagata cgcatgatga ggcaagggtg gttgcttgcg gcaatatcgg
      661 gtgtgtttct agttggctgc ggggcggagg aatccgggag ttccaagctg gccaagaaca
      721 aggaggttgc gtcgtctcgc gtgagtcagt cgctcacctc gaatctcgag acagagactc
      781 ggggcgcggc gccggacgcg ggctgtgcac tgctggataa tccagagctg cggccgcgca
      841 tgagcgcggc gctcgagaat aagctgctgg ttctctgcgg tcgtgctcag aagtcggcgc
      901 aagtcacgac cgttcccggt gggctgccta cgcgcgcacg gcgtgcagcg gcggcgctct
      961 ctgcagatat tcctgttagt gatccggcgt tggataccgg tggttccacg cagagcgaga
     1021 cgagcgtcgt ggccgtggga aacatcgtat gtgcggcctg gaacgacgct ggtgagggag
     1081 ccgggctgaa cgggtttgca ggcttcggtt tttcgttgga tggcggtttg acgttcaaag
     1141 acggcggtcc tttcccgatc ggtccggggg gagatcagag cttcggggat ccgagcctgg
     1201 cctacagcgc ccgagataag gctttttact ttgcgtcact cagcagtcag gggttgtcgc
     1261 tctggcgttc actcaatggc tgccaaagct tcgagtacgt gggtgccatt caaagcggct
     1321 ttggacacga caaagagttg attgccgtcg acaatgaccc agggagcccg tatttcggtc
     1381 ggatccacat gggttggacg gcgttcggtc agtcgaacga cctcaacgtg acctcttatt
     1441 cggatgacgc cggcaagacc tgggcgcccg tcgctcattt gcccaactcc ggtggtgccg
     1501 ggcagggcgt gtatccagcg gttgctccga atggcgacgt gtacatggcg ctcgtgaacc
     1561 gcgcgttcaa tatcggtggc gtgcaggacc agtggattta tggctcgaaa gatggcgggc
     1621 aaacttggtc caagctggcg gacatcggca cggcgcagct ccagcccgag gacgccaacg
     1681 ccactcaaga ttgtggtcgt caggcgctga atggccacat caggaacctg tcttcccccc
     1741 aaatcgtggc cacgccagac agcagcgcga gcgcaggcta cgtaatccac gccatttacc
     1801 cgtacgactc cgatggtgcc gggcccgatc acagcaacgt attctatcga cgttcgagcg
     1861 acggcgcatc gacctggtcg gccgaagtcc agctgaacga cgacggcacc aataccgatc
     1921 agttctatcc cgcgcttggc gtcgacgaat ccggggtgct ggccgcgagt tggtacgatc
     1981 ggcgtctaga cccactcgga aatacttcgt tcgatcgcta tctcacggtc tccgccgatg
     2041 gaggtttgag ctggtcaacc aacgagcggg tgagtgacgt aagctcaccg gtggcgcaaa
     2101 cactgcccaa cttcgatggt ttggccagct gctatcatgg ggactacgat caaatcgcgg
     2161 tctctggtgg cgtcgctcac ctcatctggt ccgatgatcg gcgagtcacg gagttggggc
     2221 ctaaccctga cgtttactac gaccaattcg ccatcaacgt tcacttgggc aaaatccgag
     2281 ctcagcaatc gggggtgagc tgctccagca gactcagctt tagcttgacc gatcaagacc
     2341 tggcgggctc gggcgcgcac gagatcaccg tcactacaac gggcggtgat agcgagacct
     2401 tggttttgac cgaggagggg agccgtccgg gtgctttctc gggagccatc gccacgactt
     2461 cagggccgct tcaactcggt aacggctcgt tacaggtcga agacggcgcg acgatcaccg
     2521 ctacctactt ggatgccgac gatggcaaag gcaaccctgc cgtcagcacc gccgaagtgc
     2581 gagtcgactg cgctcctcca gtcttgcaaa atgcgcgcgt ggtagcactc accggcacgg
     2641 gtgcgaccat caccgtagac tcctcggagt ccgcggcgtt gacgattcag tatggctttg
     2701 cttgtgccga tttgactcag agcgcgactt cgagcgttgc cgcgataccc acggtggccc
     2761 tggacggttt gtactccgga atgacgtact tctacgcgct caccgccacc gatgcgtttg
     2821 gcaacagcgt gacggacgac aacgatggca gctgttacag cttcaaaact ctggatgtac
     2881 tctattcggt caacttcgag gctgggctcg ctggcttcga gatcgacaat ggcagcacga
     2941 ataccggggg ctcaggcggc acaggtggtt tcggcggcaa gcccagcttc agcggctcct
     3001 tgggcgcagg cgggattccg agcgtcagtg gagccccggg ccagggcggt agttctggta
     3061 gcggcaaagg tggtgctccc gggacgggcg gcacgagctc ttgggaggaa ggaggggttg
     3121 gcggtgccgt ggaagaaggc ggaagcgttg gcactggcgg ctcatccggc agcagcggta
     3181 gtacgtcccg caacggactc tggcatttga gtcaaggttg cgccgctgca ttggttggac
     3241 actctcgccc gttcgccctc tactacggac aggacagcac ttgtacgttc gatacgggca
     3301 ccatcaacga gggctttgcc acgtctcccc cgatcctcct ttccgatgcc agcttcgcga
     3361 cagtggagtt caactacttc ctcggcaccg agggcggggg gttctatgac caagcttctc
     3421 tcgaagttag tgtcaaccag gggccgttcc aggtcgtggc gagcaacttc acgagtctcc
     3481 tcagcgcggt aaaggcgatt cgcaagcgtg cggacgcgcg ccccgcgggc cggtattcgc
     3541 tcgtctccaa tcggggcgag tgggaacatg cgaccgccga tctcactcca tttttgcagg
     3601 ggctcactca ggcggagatc cgtttgagat tccacttcaa cagcgtcgac tcgttcgcga
     3661 acgccttcgc cggattctat gtggacgaca tcaaggtgct cggcgttcag gcgccaatcc
     3721 catgtcgaca aaacgcggat tgtgacgatg gtctgttctg tacgggcaat gaaacctgta
     3781 gcgcgggctt ctgcgccaag ggtctgcccg tggtctgcac gggccctgaa gatggcgtcg
     3841 cctgcacgca gctgacgtgt gatgaaacgg cacgcggatg tatcgagaag ccaatcgacg
     3901 agctttgtga cgatggcacg ttctgcaacg ggccggagcg ttgcgacgtc accttgggtt
     3961 gccagcccgg agcgccggtg gtctgtggcg cgagcaaagt cgactgcgtg atcagccaat
     4021 gcgaagagcg ggtgaagaat tgtgttcagt tccctgacca cgcgcggtgc gacgatggtt
     4081 cgttctgcac gggtcaagag ttctgcgata tcagtactgg ctgcgtcgcg ggtagcccgc
     4141 cttgtcagga tccagtgagt tgcaccgatg acctgtgcga cgaggcgacg ttttcctgta
     4201 acttcgtgcc gaacgacacg tattgtaacg acgggttgtt ttgcaacggc gccgagtact
     4261 gcgacggtta tcagggctgc cgcacgaccg gatcgccctg cagtgcctcg gacaagtgcg
     4321 acgagaaggg cgaccagtgc gtcccaatct gctttacgga cgtcaactcc aaccatcaaa
     4381 ccgcgggtcg cgcttacgcg aagaagaagg ccttttatgc gctcggttcg ggggacgctc
     4441 tcggcacggc atcggaggtg acttcgctgc agggtagtgg cactttctgg aagcacgtgc
     4501 cgagctgccc agctcccccg agcatcgata gtgttcaggt cagcgtgtct ggaagcgtgg
     4561 ctcgcgtcag tggcgtggca tcggacccga acggagatat cgtcaaggtg cgtctcacgt
     4621 tctggttgca tggctcgttg caaatcgtgc tcgatgccaa ggggaccacg aattggagcg
     4681 gcgacttggg cttgcccgag ggaacccacg ccgtcagtgc ccaggccgtg gatcgggcgg
     4741 gattcgtcag tgcatggagc gacctctact tcttcaccgt gctgccagcg ttgcctcccg
     4801 tgctaaagtc tgtcgcagtc agcgtgaaag gatcgaatgc gattgtgtct ggcagcgcgg
     4861 gcgatccaaa taatgatatc cagtacgtcg aggttacgat tctgcagaac aacgagatcg
     4921 tcgcgtttcg taaggctcag ggaaccagcg atttcagcgc aattatactg ggtttggcgg
     4981 caggcaagta cagcgcgcgc gcccaagcct tcgatagcac cggcttcgcg agcgctctga
     5041 ccgacccagt tactttcgat atcgcggccg agcagtgcgt cacggatagc aacggcgggc
     5101 acaagagctt cgggcgtgcc aaatcggctc agggcaacta ctatgcagtc ggttcgggtg
     5161 acgccctcgg tcacaaagcc tcgaccatca ccgcgctgag tggctcggcc gagttctgga
     5221 cgcgggttgc tcgctgtccc taaaccaaga atgacgaaca aagacaaccg caaacagagc
     5281 ccgccgaagc cctgaacggt atgaaacagt gcggggcgcc agagtcatac tgcgaacgat
     5341 aggagtcgaa catgcgcccc gcaccactcg tcaggatccc caacgtcgtc actctagcca
     5401 ccctcactgc gtgcctggga gtgcttgctc cggcgtgcga gtcagacgac agctcgtccg
     5461 cgcccacggg tggcttgcat tcgggtggcg gcacggtccg ctccggcggt acgagccact
     5521 ccgtcgctgg cgctagctct ggtgctggcg tcaactctgg cgctgggcgg caggacgccg
     5581 gtcatggtgg cgatagtggc ggggcctcga ccggtggcaa cactgaagcc gaggggggca
     5641 acgccgagac gcagggcggc tcgaacgcga acacctcgaa cggcggcaat ccccaaagta
     5701 cccgcggcgg agcgaacaac acgcagtcct ccggagcaag tgcgtccggg ggagccgccg
     5761 gagcggactc gatcggcggg gctgcgggcg acgtaaactc tggaggtctc accaattcgg
     5821 gaggaaccct gggcggggga atgacgagcg tcgtttcggg gggcagctcg aacacctccg
     5881 gcggcaccgc gaacacctcc ggcggcgcgt cgggcggtac ctcgaactcc gcgggggcca
     5941 cttcggcgcc taactgtgaa gctctgagtt gtgtctcagg ttcgagttgc caatgttgtc
     6001 cgcgcggcgg cccgacgcag atctgcattt gctccaccgc gtgtcagggc gactccgatt
     6061 gcctcgatcc gtcaaccccg tcttgcagca aagcaccggg agccgcctct gggttttgca
     6121 ctgcggagct caagctgtgc tgttggcaat gtgaatgaca cgattcgata tggcgcaggc
     6181 actgagctcg ctgtttccga tcgtgacggg tccgaagatc ggaaggaacg cgccatgccc
     6241 ctgcggcagt ggcaagaagt tcaagcggtg ctgcgaaggg aaagcggcgt aaggtttttt
     6301 accgaaggcc ggcgatgacc gctcaattgc cggccgaaat cggtggtcga ccgatgcggc
     6361 gtccccatgg aatcccggcc ttgctcgctg ggcggcggtc cttggttcgt aagcgcacgg
     6421 gttggatgtt gcgccggcga atatcgctcg atagcattcc ggcatggcca agggtaatca
     6481 cgtcttcaac cacttcgaga aaaccatccg gaacgcgttc gaggcctttt tgactgaggg
     6541 cggggttcag gtcgaagatc gcgcggtgga cgtgaactcg gccattgcca tgccgggttc
     6601 ggcggacgcc gaggcgatcc gcgcgcgcta tccgcgggcc tgggcgaagt tcaaggaaat
     6661 cgtgagtgcc ccgcagcaca aggagatgga gggcatgcag gatgactcgc ccgtggccgc
     6721 caacgaccct aactcccaga gctacggaat cgcggctgcg cagttcatct ccggagtgcg
     6781 tgcgttggcc agcgcgcaac cggggatgcc gccgggcgcc gcttggcagg ccatggtgaa
     6841 gtcagggttc gatgcggcgg ccgccgatcg ttacgtcaac gccgcgcctc gtgccggcca
     6901 ggcaaaacga aaggctgaca tcgagaggat acggaccttc atcaaaggtc gcctaaactc
     6961 ggacgtctct gtttggatcc agttcatgaa gctcgaccac gcgggagaga agtgaaacgc
     7021 gaggatcgtc tcagcgaatg acaacgattg agctcgagtt ggaagtgatg agttggagca
     7081 agccgcaagc atgcctgcgg tggtgagtcg tccggtccgt ttcttccccg agggtcggcg
     7141 gggtggtgag aagagctgtg tgacgggaat gagcgagacc agagcatcct cgtcggcatc
     7201 actctcgccg acttcaacct tctcgacgag tacttcggct attcagttac cgacggtgcg
     7261 gacccgcgag tcgacttcaa cgatgacgga tgggtggatg agttcgatta tttggtaatt
     7321 gcggaggatt gggccaaggg ttgcgaatag ggttgcatcc agacgcgcaa gcgcgtggtt
     7381 aggcggcaac ttgatcggtg tgcttggcgc atcttgggtc agagctgtgt ctcgccaccc
     7441 caagggcgcg tccgccggga cacgccggcg accaaccaat gccgagattt ctcgggattg
     7501 agtgcgatgc gcgatcggca cgtggcttgc gatagcagcc ggtatgtcat cgtgcttcgt
     7561 gccgggtagc caccgcgctt cccgctttct cccgcgccgg gctctgctcg cggcgacgtt
     7621 gtttctaggc ttgctcgagc aggtctcatg ggcggccccg actgtcgacg atacgcgcgc
     7681 gccggtaaga tcccgcgctg ccccgaaact tagcgtccag gcggtcgagc gggagccggc
     7741 gcgaaagcga ccgagcgccg aggagctcgc gacacttcgg cggggggtcg aagagaatcc
     7801 cgcgctgcgt aaggctcggt tcgagctctc gcatgccctg ttgcgtgccg gagatttgca
     7861 ggcggcccac cgtgaagcgg aagcctggcg tgagcacgac gcctacaact tggtggtcgt
     7921 tcgcatgctc ggtgacatcg agagtgagct cggggatcgg gagcgggccc gcaggacgta
     7981 ctcggcgatc gtggagctcc taccaaagga tgtggaggcg cggcgcgcgc tctcgaccgt
     8041 actcaagcag gcgggcgacc tgcaagccgc gcgcgccgaa ctcgtggccg cgctggcgct
     8101 cgcgcccaac gatgcgcgca cgcagttcga gctcggtgat gtcgagcagc ggctcggcat
     8161 gcaggattcc gcgcgcaccc gcttcgagac caccaaggac gccgccgatg ctcccgaatc
     8221 actgcgttat ccggctcgcc aacggcttgc ccagatccac gcgcagaaac ggctcgaggc
     8281 cggccgccgc ggggacacga accaagttca cgcgctcggt caggccattg atgctctgca
     8341 gatccacggt gggaccgaga acgatatcaa ggtttatctg tcgtgggaca gcgaccgtac
     8401 ggacgtggat ttgtgggtgc taactccctc gggagagaag gtgttttacg cgcaacgtca
     8461 aggcaaaggg ggtgaggcgt tgttcgatga cgtgacgacg gggtatggcc cggagagctt
     8521 cacagctccc cacgccgagc ccggcaccta tcgagttcag gtcaactact tcggatcgcg
     8581 cgccagcacg ttcaaggagg cgcgtggtga agtgaccgtg attttgaacg agggacgcgc
     8641 caccgagcag cggagtgtgt tcccgtatcg cctgttcgaa gagaaggaca cgttgacggt
     8701 ggctgacatc cgcgtggagg cgaagccgtg agttcgaacc ccgccgtccc gtcactccaa
     8761 tccaggaaat ttgcaatgaa tcgcgctcgc tttggaacac ttgcactctt gatcttactt
     8821 gccctgcagg gttgctcgaa cggtcacgtt gggagcctgg gtcctcacgc cgccgcctcg
     8881 gcgggttcgc cgctgatcga cgacgagccc gaccctgctg acgcgggcgg catcggttgg
     8941 cttaccgcac gcaatgaacg cggtgagggt gggctgattg aactcgccca ggtcaacatc
     9001 gacgccgaac agattggcga ctacgccgcg gtctcgatcg agcaccgttt cgtgagccgc
     9061 gcgacgaccg tgcaggaagg cacttttcgc ttccctctgc ccgaggacgc gctgctgacc
     9121 ggtctgagca tgttgatcga cgggcgcgtg gtgcacggtg agttgctcga gcgcgagaag
     9181 gctcgccgca tctacgagga gattgtggac tccatgcagg acccggcgct gctcgagtgg
     9241 gagcacggtt cgaccttcaa gatgcgcgtc ttcccgattg agcctcagca acccaaactg
     9301 gtgacgattc ggtatttggt gccgctgaag cacaaaggca agcagtgggt cttcagccaa
     9361 ggcacacgcc agaacgcagg cgctggcaag ctgagtgaac tgacgttgcg ttggcagggc
     9421 aaacaggtcg tcagtgagcg gggcgtgcaa gccggtcgga tcctggaagc gacagccagc
     9481 gatccttcgg atgtgctgcg ggaagagagg cccgaggccg catatacggc ggtgcgcgtg
     9541 cgcccggatt gggcccaggt acccgtgcag acccgaacgg cacccaagca ctgggtgttc
     9601 gtggtggata cctcgcgtag cgccctcgaa gagcgcaagc tgcagctcga gactttacgt
     9661 accctgctcg ccgcgttggg cccgcaggag cgctttaccc cg
//
LOCUS       DASTKU010000904         9792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1740567, whole genome shotgun sequence.
ACCESSION   DASTKU010000904 DASTKU010000000
VERSION     DASTKU010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9792
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740567"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFQ61_31655"
     CDS             complement(<1..266)
                     /locus_tag="VFQ61_31655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959104.1"
                     /translation="MDRLRHRGSKLGWPKSAALLSAIAVVVGMGLGARYILVRDTPTS
                     VRPRVGHPNAEPAKRAASPMPPPVLEEQLRAAKEEDGCRLRHRGS"
     gene            460..1377
                     /locus_tag="VFQ61_31660"
     CDS             460..1377
                     /locus_tag="VFQ61_31660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9959105.1"
                     /translation="MAPRSSKSRSANGSKLSSKHSPTHRKGAGKKSRKARETPEPNGS
                     DTQSARVVEAKGKGDIETPGLRTLSVDIGGTGIKMIVLDEKGQPITDRIRWLTPQPAN
                     PKNILEVIKQMLSAVGRFDRVSVGFPGVVVGGVVQTAANLDSKAWKGFDLEKALVRIT
                     QVPSRVMNDADLQGFGVIEGRGVELVLTLGTGLGSALFVDGILVPNLELGHHPFMKGK
                     TYEQRVCDAAYHRLGKKEWSQRVGEMIDQLAPILNYDVMHLGGGNAQNLRLKLPRNVK
                     LFDNVDGMTGGIRLWELGERHKLASATNH"
     gene            complement(1676..4114)
                     /gene="lon"
                     /locus_tag="VFQ61_31665"
     CDS             complement(1676..4114)
                     /gene="lon"
                     /locus_tag="VFQ61_31665"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET9959106.1"
                     /translation="MSEKKKPTPPQAPPPSEEDIRFGDELPVLPIRNAVLFPGSVAPF
                     DVGREKSVALVEDVHNLSNPVIAIFAQRDPATDDPSADDLYPVGCAARVLKALKHSSG
                     NYSLILQGLTRIRFSGMTQGSPYMRARITRIDTPPTEDVEAEALAMSLRDVAKQVIQL
                     MPELPREAGSLIDSIQAPGALADLVASNLDAPVEEKAQLLETVEVKDRIRKVLKLLTR
                     QLEILKMRERINSQIKEEMGKNQREYVLRQQLKAIKEELGEDDGDQGDLDGLEERIAK
                     ANLPSEADSVAKKQLKRLRSMQVGSAEYTVVRTYLDWILDLPWSHSTEDNMDIAEVRR
                     VLDEDHYGLEKVKKRIVEYLAVRKLKKDKKGPILCLIGPPGVGKTSLGRSIARALGRK
                     FVRVSLGGVHDEAAIRGHRRTYVGALPGQIIQGMKKSGTINPVFMMDEVDKIGHDFRG
                     DPSAALLEVLDPEQNNTFADHYLEIPYDLSNVMFVATANVADPIPPPLRDRMEILEIP
                     GYTRREKLAIARQHLIPKQLDEHGLKSEQLTITDEAVEEVIEHYTREAGVRSLERTIA
                     SVIRGVAVKVAEGDSTARVIKSEDDLREFLGPIKYTSEVAERTEETGVATGLAWTSVG
                     GEILFIEVTRMFGTAKMQLTGQLGDVMKESAHAALSYVRTNAEKYGIPKDFLEKSDIH
                     IHIPAGAMPKDGPSAGITMFTAIVSLLTGARVRHDVAMTGEISLRGRVLPIGGVKEKT
                     LAAHRAGIKRVILPERNKADLEEVPKEVRDELEFVFVNKLDEVLAAALERMPQPSQAF
                     LDEVAKRETQPSTN"
     gene            4630..5583
                     /gene="fmt"
                     /locus_tag="VFQ61_31670"
     CDS             4630..5583
                     /gene="fmt"
                     /locus_tag="VFQ61_31670"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET9959107.1"
                     /translation="MRAVFFGTPALAVPTLRALCDISEVVGVVCQPDRPAGRGLVPQA
                     PAVKSAALELGCEVYQPEKVRNGELERWLREREPDVAVVLAYGRILPKGVLEAPRRGC
                     VNLHASLLPRYRGAAPIQWAILRGERETGISMMQIDEGLDSGPVFSRHAIPIGENETA
                     GELAERLANLGAEILRAELATIVEGRLTSQPQDPALATLAPPLERVHGRLDWSRPAQQ
                     LHDQVRGLAPKPGAFTSCRGKQVRLARTHWVATAPALLPGQVRVERPRVLIGTGDGAI
                     ELLRAQFEGKKELDALSLVNGRYINDGDLLGDEPKPTTGPG"
     gene            5688..6338
                     /locus_tag="VFQ61_31675"
     CDS             5688..6338
                     /locus_tag="VFQ61_31675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959108.1"
                     /translation="MANANLLRTPAGEILIAPETSRPLSAALLRDIAREFPRFRVVSK
                     HESRLCRAIHAALIVLTLGGQRRFLDGYYTVLFDTLYVPTTAWEALDDIDRVILLRHE
                     RVHLRQRRRYGGLGMAFIYLVPIFPLGLAWGRARLEWEAYAETLRATAELRGLPAAQS
                     ATLRNKIIDRFVGPDYGWMWPFRSTIERWYRRVLEELASQPGARLGSTPASNPFQK"
     gene            6387..8747
                     /locus_tag="VFQ61_31680"
     CDS             6387..8747
                     /locus_tag="VFQ61_31680"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET9959109.1"
                     /translation="MSEVIGIDLGTTNTVVAAVREGRAVTLPAEDGSTLIPSVVSFHP
                     NGSVLVGNPARARRQIDPQNTIYSVKRLIGRSWETAEVRTARSRFPFEMREGPGQATL
                     VVGRGENYSLPEISAFVLRRAKQVAEAALGATVSRAVITVPANFNDLQRAATKVAGRV
                     AGLEVLRILNEPTAAALAYGFGKGSSERIAVYDFGGGTLDVTLLDLSDNVFEVLATAG
                     NTFLGGDDMDAVLLDHMSGGFQSRYRYDPRSDPILLEKMRAAAERLKRELSFADEASV
                     VVEEAAPDPRGGTSEFEFWLSRGAFEHLVSPIVDRTFEVCREALALAGLTVRDFDQVL
                     VVGGSTRIPLVRRRVEEFFQREVRSDVSPDEVVALGAAIQANALTTQERRKTSLPPPP
                     LAFRRTLPGAPSEPPRLEAFPNLGTPAFGTPVSGAPNLGTPVSAHLSGTPSSPPPTSQ
                     APPPSARLVFKTPPTELIPDELPSVELPPEDEITVNQSHPLFSEEPTLARGSVNSPDL
                     VVPDPSGLIDHPGLIRPTPVLVNPEGPPSSRAPGPGLAPPFEIPGAPLSSPFSSTAQG
                     AHHPLNARTAMASEPPLPPPVPNREGMDFRTFPVAANHPGADGESQVTRPPQFDRAPQ
                     FDRAPQASTVSLPGSVTLHPQMPLLVDVTPLTLVVETVGGFCDAVIARNSQIPCAETR
                     LFVTAVDGQSVVRVRIAQGESKRFEENTLLGEVELLGIPPAPRGSIQISVTFALDASG
                     LLSVSAVESGSGKASIARLNLLGLPNADGVEGSRFGAFIASSSPPP"
     gene            8796..9338
                     /locus_tag="VFQ61_31685"
     CDS             8796..9338
                     /locus_tag="VFQ61_31685"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET9959110.1"
                     /translation="MASTDLSQADLSHLLAWEQSLDVLSYYELLGVLEVADESDIRQQ
                     FREFCRAFHPDSHPHGGSEVANRLERIFQRGAEAYRVLMNPELRASYDLGLARGRLRL
                     GAEAPSVAPRNQIRTLDELCQSAGGKLHARRADKLISEGDLERAKRELLLAWRSEEQP
                     DPELADRLDALDLALFARGA"
BASE COUNT         1778 a         3157 c         3020 g         1837 t
ORIGIN      
        1 gacccgcggt gccgcagccg gcaaccgtcc tcttccttcg ccgcccgcag ctgctcttcc
       61 aagaccggag gcggcatggg cgacgcggcg cgtttcgctg gctcggcgtt cgggtgccca
      121 acgcgcgggc gaactgaggt cggagtgtct cgcaccagaa tgtaccgagc tcccagcccc
      181 atgccgacca cgaccgcgat ggcggacagt agagcggcgc tctttggcca gccgagcttt
      241 gacccgcggt gccgcagccg atccatccga ccgctcagtg tcgcgccttc ccctgtgaaa
      301 ggcaactgat cggcgtctac cccctgatgc gtaaggacgc cgtaatcacg aaggtgcccc
      361 ccgtgtcgcc gtcattggag ggggtgtcag cgggcgggtg attccggttg ctagttcttt
      421 cgtggcagcg gattcagacc aggctaacct cgggcaccga tggctcctcg ctcctcgaaa
      481 agtcgttctg caaacggcag caagctctca tccaaacaca gccccacgca ccgcaaaggc
      541 gcaggcaaga agtcccgcaa agcgcgcgag acgccggagc cgaacggctc cgatacgcag
      601 agtgcgcgcg tcgtcgaagc gaaaggcaaa ggcgacatcg aaacgcccgg gctgcgcacg
      661 ctgagcgtcg atatcggtgg aacgggcatc aagatgatcg tgctcgacga aaagggtcag
      721 ccgatcaccg accgcattcg gtggctgaca ccgcaaccgg ccaatccgaa gaacatcctg
      781 gaagtgatca agcagatgct ctcggccgtc ggacgcttcg atcgcgtgag cgtcggcttc
      841 ccgggggtgg tcgtgggcgg ggtcgtgcag acagccgcca acctggattc gaaagcctgg
      901 aagggcttcg atctcgagaa agcgctggtg cggattacgc aggttccctc acgagtgatg
      961 aacgacgcag accttcaggg gtttggcgtg atcgaggggc gcggagtcga gctcgtgctg
     1021 acgctcggaa ccggcctagg ctcggccttg ttcgtggacg gaatcttggt gcccaatctg
     1081 gagctcgggc atcacccgtt catgaagggc aaaacttacg agcagcgcgt gtgcgacgcc
     1141 gcctatcatc gcctcggcaa gaaggaatgg agccagcgcg tcggagaaat gatcgaccag
     1201 ctcgcgccaa tcctcaatta cgacgtcatg cacctcggcg ggggcaacgc tcagaatcta
     1261 cgcctcaaac tgccgcgcaa cgtcaaactg ttcgacaatg tcgacggcat gacgggcggg
     1321 atccgccttt gggagctcgg tgaacgccac aagcttgcga gcgccacgaa tcactaatca
     1381 atcagcccgc tgaggctgtt tcgaggatcg aaaaaggcaa gagccgggcc gccctaaggc
     1441 gtcaccggct cttgtttcgg gtcgctcgcg ctgttgaagt cgacgatcgg agggtcgcgg
     1501 aggaacagta gcggaggaac acacttcgga ggaacacttc gcaggacgca gcgcgagcac
     1561 cgaaatttgc gctgatccag ccgctctccg gtgtcttaca ccaagaagag tcgcgctggc
     1621 ggtgggcccg aggggccggc gtggcgacgc cagcccttcg gttcgcccgg ggtggttagt
     1681 tggtggaagg ctgcgtctcg cgctttgcca cttcgtcgag gaaagcttgg gacggttgcg
     1741 gcattcgttc gagcgccgcc gccaacacct cgtcaagctt gttgacgaac acgaactcga
     1801 gctcgtcgcg cacttccttc ggcacctctt cgaggtcggc cttgttccgc tccggcaaaa
     1861 tcacgcgctt gataccggcg cgatgagcag cgagcgtctt ctctttgaca ccgccaatcg
     1921 ggagcacgcg gccgcgcagt gagatctcgc ccgtcatcgc cacatcgtgc cgtacacgcg
     1981 cgccggtcag caaagacacg atagcggtga acatcgtgat gccggcgctc ggtccgtcct
     2041 tgggcatggc gcccgcggga atatggatgt gaatgtcgct cttctcgagg aagtccttgg
     2101 ggatgccgta cttctcggcg ttcgttcgga catagctgag ggcggcgtgg gcgctctctt
     2161 tcatcacgtc cccgagctgc ccggtgagct gcatcttggc cgtgccgaac atgcgtgtga
     2221 cttcgatgaa caggatctcg ccacccacgc tcgtccaagc caagccggtc gccacaccgg
     2281 tctcctcggt acgctccgcg acttcgctgg tgtacttgat gggccccagg aactcgcgca
     2341 gatcgtcctc gctcttgatc acgcgtgccg tgctgtcacc ctcggccacc ttgacggcca
     2401 cgccgcgaat cacgctcgcg atcgtacgct cgaggcttcg aacaccggct tcacgggtat
     2461 agtgttcgat cacctcttcc accgcctcgt cggtgatggt gagctgttcg gacttgaggc
     2521 cgtgctcgtc cagctgcttc gggatcaagt gctgacgcgc aatggcaagc ttctcgcgtc
     2581 gcgtgtagcc cgggatttct aggatctcca tgcggtcccg caaaggcggc gggatcggat
     2641 cggccacgtt ggcggtagcc acgaacatga cgttcgagag atcgtacgga atctccaggt
     2701 aatgatcggc aaaggtgttg ttttgctcag ggtccagcac ctctagcagc gcggcgctgg
     2761 gatcgccgcg gaagtcgtgt ccgatcttgt ccacctcgtc catcatgaac acggggttga
     2821 tcgtgcccga cttcttcata ccctggataa tctgcccggg aagcgcgccc acgtacgtgc
     2881 gacggtgacc ccgaatcgcg gcctcgtcat gcacaccacc gagggacacc cgcacgaact
     2941 tgcgtccgag cgcgcgcgcg atgctgcgac cgagcgacgt cttgcccacg ccgggggggc
     3001 caatcaagca caggatcggg cccttcttgt ccttcttcag cttgcgaacc gcgaggtact
     3061 cgacgatgcg cttcttgacc ttttccaagc catagtggtc ctcgtccaac acgcgtcgca
     3121 cttcggcgat gtccatgttg tcctcggtcg agtgcgacca aggcaaatcg agaatccaat
     3181 ccaggtaggt gcgcaccacc gtgtattcag cgctgcccac ctgcatcgaa cgcaggcgct
     3241 tgagctgttt cttggcgacg ctgtccgcct cgctcggcaa gttggccttc gcaatgcgct
     3301 cctcgaggcc gtcgagatcg ccttggtcac cgtcgtcctc gccgagctct tctttgatcg
     3361 ccttcagctg ctgacgcagc acgtactcgc gctgattctt gcccatctcc tccttgattt
     3421 gggagttgat gcgctcacgc atcttcagga tctcgagctg ccgggtcagc aacttgagca
     3481 cctttcggat gcgatccttg acctcgacgg tctctagcag ctgcgctttt tcctcgacgg
     3541 gagcgtccag gttcgaggct accaagtccg cgagcgcgcc aggcgcctga atcgagtcga
     3601 tcaacgaacc cgcttcgcga ggcagctccg gcatcaactg gatcacttgc ttcgccacgt
     3661 cccgcaagct catcgccagc gcttccgcct ccacgtcttc cgtgggcggc gtatcgatgc
     3721 gggtaatgcg cgctcgcatg tacgggctgc cctgcgtcat gccgctgaaa cgaatgcgcg
     3781 tgaggccctg gaggattagc gagtagttac ccgagctgtg cttgagcgcc ttcagcacgc
     3841 gcgcggcgca accaaccgga tacagatcgt ccgcgctggg atcgtccgtc gccgggtcgc
     3901 gttgcgcgaa gatcgcgatc accgggttgg agaggttgtg tacgtcttcg accagtgcga
     3961 cggatttctc gcgaccgaca tcgaagggcg caaccgagcc tgggaaaagc accgcgttgc
     4021 ggatgggcag caccggcagc tcatcaccaa accggatgtc ctcttcactc ggcggcggag
     4081 cttgcggcgg agtcggcttc tttttctcgc tcatggcttc ctcatcatgt caccccgtcg
     4141 acccgtcgcc cacctggtaa aggaggtagc ttcgaccgcg gccaggaggc tcgaaacgcg
     4201 ccgcggagag cttgggcaat tctgttcacg tgctcgtcca cgtgtccgcg cttctgcgcg
     4261 tgcccaatct cttcctgaaa cggcgaccat tggcagaacc taaccacctc gcggcccgag
     4321 gccaagtcgg aacgcgccag gttcgattga acggccctct gttcgctacg catccaacac
     4381 gctttcggtg tcgggggtct cgctcacgct agagcgctgg agcgtcgcgc gagccacgcc
     4441 cggttttgac tcacccactc tgcgcggctc gttcaaacgt ccgttctcta ttccgagagg
     4501 gggtttcaag attggacttc ggagggccat gcggctccga atgtgtcggc acatggatct
     4561 gaattgccgg gcccacgcct cggtccggaa atctgagcgt cgcttgagtg aggcgtgtat
     4621 accggcccca tgcgcgctgt tttcttcgga acaccggccc ttgcagttcc cacgcttcgg
     4681 gcgctgtgcg acatttcgga agtcgtcggt gtggtgtgcc aaccagatcg gccagcaggt
     4741 cgaggcctgg tgcctcaggc gccagccgta aaaagcgccg cactcgagct cggctgtgag
     4801 gtgtaccagc cagaaaaggt gcgcaatggt gagctcgagc gctggctacg tgagcgcgag
     4861 cccgatgtgg ccgtggtgct ggcttatggc cgaatcttgc cgaaaggcgt gctcgaagcg
     4921 cctcgccgcg gttgcgtgaa cctgcacgct tccttgctgc cacgttatcg cggggccgct
     4981 ccgatccagt gggcgatctt gaggggcgag cgagaaacag ggatttcgat gatgcagatc
     5041 gacgaaggcc tagacagcgg ccccgtgttc tcgcgccacg ctattccgat cggggaaaac
     5101 gagacggcag gtgaactcgc cgaacgtctc gccaatctcg gtgccgagat cctgcgggcc
     5161 gagctagcaa caatcgtcga gggccgcctc accagtcagc cgcaggatcc agcgctcgcg
     5221 acgctcgccc cgccgctgga gcgtgttcac ggtcggctcg attggtcccg accggcccaa
     5281 cagctccatg atcaggttcg agggctcgcg cctaaaccgg gcgcgttcac gtcatgccgc
     5341 ggcaaacaag tccggctggc gcgcacacat tgggtcgcca ccgctccagc tttgctcccg
     5401 ggacaagtcc gggtggagcg gcctcgcgta ttgatcggga ccggcgacgg cgcgatcgaa
     5461 ctgctgcggg ctcagttcga aggaaagaaa gagttggacg cgctctctct agtcaacgga
     5521 cgctatatca acgacggaga cttgctgggc gacgaaccga agcccacgac tggcccgggc
     5581 tgatccactc tacgctgggc agaggcgggc cagcacagct gccagcacag ctgagccaac
     5641 acagctgagc cagcccggcg catgcagtct gagccgatgc caatctcgtg gccaacgcta
     5701 acctactgag gacgccagca ggtgagatct tgatcgcacc cgaaacctcc cgccccctca
     5761 gtgccgccct tttgcgagac atcgcgcggg aatttccgcg cttccgggtg gtgtccaaac
     5821 acgagagccg tctctgccgc gccatccacg cggcactgat cgtactgact ttaggtgggc
     5881 agaggcgctt cttggacggc tactacaccg tcctgttcga taccctctat gtgcccacga
     5941 cggcttggga ggccctagat gacatcgatc gcgtgatcct gctccgacac gaacgagtgc
     6001 acttgaggca acgccgccgt tacggtggcc tcgggatggc ttttatctat ctggttccca
     6061 tcttcccgtt ggggctcgcc tgggggcggg ctcggctgga gtgggaagct tacgccgaga
     6121 cgttgcgcgc gaccgctgag ctacgtgggc tccccgcggc ccaatccgcc accttgcgga
     6181 acaagatcat cgaccgcttc gtgggcccgg actatggctg gatgtggccc tttcggtcca
     6241 ctatcgagcg ctggtatcgg cgcgtgctcg aggagctagc gtcccaacca ggcgcgaggc
     6301 taggctcgac cccagcatcg aacccgttcc agaaatgatc cgcacgacgc aatgcaatgc
     6361 ggcccaatgc aaggccagcg aatccaatga gtgaggtcat cgggatagat ctcggcacga
     6421 ccaacacggt cgtggccgcc gtgcgcgagg ggcgcgccgt cacattgccg gccgaagacg
     6481 gatccacact cattccctcg gtcgtctcgt ttcacccgaa tggcagcgtg ctggtgggca
     6541 acccggcgcg cgcgcgccgg caaatcgacc cgcaaaacac gatctactcc gtaaaacgtt
     6601 tgatcgggcg cagctgggaa accgccgagg tacgcaccgc acgctctcgc ttcccgttcg
     6661 aaatgcgcga aggtccgggc caggccacgc tcgtcgtcgg tcgcggcgaa aactacagtt
     6721 tgcccgagat tagtgccttc gttttgaggc gggccaagca ggtcgccgaa gcggcgctcg
     6781 gcgctaccgt cagtcgggcg gtgatcacgg ttcccgcaaa cttcaacgat ctgcagcgtg
     6841 ccgccaccaa ggtggccgga cgcgtagcgg gccttgaggt cttgcgtatc ctgaacgaac
     6901 cgaccgcggc ggctttggcc tatggtttcg gaaaaggcag ctccgagcgc atcgccgtgt
     6961 acgacttcgg tggtggcaca ctcgatgtca cgctgctgga tctatccgat aacgtgttcg
     7021 aggttctggc cacggcgggc aacacctttc tgggcggaga cgacatggac gcggtgctgc
     7081 tggaccatat gtccggcggc tttcagtcgc gctaccgcta tgatccgcgc tcggacccga
     7141 tcctgctcga aaagatgcgt gcggccgcgg aacggctgaa gagggaattg agcttcgcag
     7201 acgaagcgag cgtcgtggtc gaggaggccg caccggaccc gaggggtggc acgagcgagt
     7261 tcgagttctg gctctcgcgt ggtgcgttcg aacacttggt atctccgatc gtggatcgga
     7321 cgttcgaggt gtgccgcgaa gctctggcat tggcgggatt gaccgttcga gacttcgatc
     7381 aagtgctcgt ggtcggtggc tccacgcgca tccccctcgt ccggcgccgg gtcgaggagt
     7441 tcttccaacg tgaagtgcgg agcgacgtca gccccgacga ggtcgtcgcg ctcggagccg
     7501 cgattcaagc caacgctttg acgacccagg aaaggcgcaa gaccagcctg ccacctccgc
     7561 cgctcgcgtt ccggcgcacg ctgcccggtg cgccctcgga gccccctcgt ctcgaagcct
     7621 tcccgaacct tggtaccccg gccttcggga ccccagtttc cggggctcca aacttaggaa
     7681 ctcccgtctc cgcgcatctt tcgggcacgc cgagctcgcc gccgccaact tcccaggccc
     7741 cgccgccatc ggctcgcctg gtgttcaaaa ctccgcctac cgagctcatt ccggatgagt
     7801 tgcccagcgt tgaactgcca ccagaggacg agatcacggt caatcagtct catccgctgt
     7861 tcagcgaaga acccactctc gcacggggtt cggtaaacag cccagatctc gtggttccgg
     7921 acccatctgg cctcatcgat catcccggac tgattcgccc aacgccagtg ctcgtgaacc
     7981 cagagggtcc gccttcgagt cgagctcccg gtcccggcct ggctcctccc ttcgaaatcc
     8041 cgggagcccc actttcttca ccattctctt ccaccgctca aggtgcacac cacccgctca
     8101 acgcgcgcac ggcgatggcg agtgagcccc ctctgccccc gcccgtgcct aaccgagagg
     8161 ggatggattt caggacgttc cccgtggcgg ccaatcaccc cggtgcggat ggagaatctc
     8221 aggtcaccag gcctcctcaa ttcgacaggg ctcctcaatt cgacagggct cctcaggcat
     8281 cgaccgtgag cctcccggga tctgtaacgc tgcatccgca gatgccactc ctggtcgacg
     8341 tgacgccgct cacgctggtg gtcgagaccg tgggtggctt ctgtgacgcc gtgatcgcca
     8401 gaaatagcca aattccttgc gcggagacgc gcctgttcgt gaccgcggtg gacggtcaga
     8461 gtgtggtccg cgtgcgcatc gctcagggag agtcgaagcg tttcgaggaa aacacgctgc
     8521 tcggcgaggt cgaactgctg ggcatccccc ctgccccccg gggcagcatt caaatctcag
     8581 tcacgtttgc gctcgacgca agcgggctcc tgagcgtgag cgcggtcgag agtggcagcg
     8641 gcaaagcttc gatcgcgcga ttgaatctat tgggcttgcc aaacgcggat ggggtggaag
     8701 gatcacgatt tggagctttc atagcgtcga gtagccctcc cccgtaagct cgaccggccg
     8761 ggccagcgca tcgcgcgcct gcgccgtcga tccgaatggc ctcaaccgat ctttcgcaag
     8821 ccgacctttc acacctcttg gcctgggagc agagcctcga tgtgttgagc tactacgagc
     8881 tactcggcgt gctcgaagtt gctgatgaga gcgacatccg tcagcagttc cgggagtttt
     8941 gtcgggcatt tcatcctgat tcacacccgc acggcggttc cgaagttgcg aatcgtctcg
     9001 agcgaatctt ccagcgcggg gccgaagcct atcgggttct gatgaacccc gagctgcgag
     9061 cttcttacga tctagggctt gcgcgcgggc ggttgcggct cggcgcagaa gcgccaagcg
     9121 tagctccccg caatcaaatt cgtacactcg atgagctctg ccaatcggcg ggcggcaaat
     9181 tgcatgcgag acgagcagac aagctcatct cggagggcga tcttgagcgc gcaaagagag
     9241 agctactttt ggcctggcgt tcggaggagc aacccgatcc ggagctcgcc gatcgcctcg
     9301 atgctctgga cctcgcactc ttcgctagag gcgcctgacg agaatcgact cgaccgaggc
     9361 cgccccgccc tgactgaacg actcggctgc agacgacgac agggtctgat tttcgggcgc
     9421 gcagggatga tccgccgcag atgtcagcag ccaaagaagg atcacgagag gctgggctgg
     9481 acctgaggcg cgggttctct ggcgatagcg acgacgcact gcgcgcaccg agtcgtcccc
     9541 tcctcacgaa gaggtgcccg gagcaacgct catagagcag caatcaactt cgcgcgggac
     9601 gcagcaacgg acgagcgtca gccggcagat gtcaaccaag gcgacccagc cccctgggtt
     9661 tcatggaaga tccgtggcac gggatttttc tgaagaagtg agcctgctcg gacgtagtgc
     9721 ccctaaggta gccatactga caaccacacc gaccgcgcat ccacgcgcaa accctacgcc
     9781 ggccccccta gc
//
LOCUS       DASTKU010000905        20534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1070849, whole genome shotgun sequence.
ACCESSION   DASTKU010000905 DASTKU010000000
VERSION     DASTKU010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 20534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20534
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070849"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFQ61_31690"
     CDS             <1..482
                     /locus_tag="VFQ61_31690"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET9959111.1"
                     /translation="AGTPKERIHETVEWLWQEQPKYNLWRRPIAGMFELVRELHDMQI
                     PLGIISNSEGRLAELADELGIGQYFRVVADSGKFGIPKPKAEIFLYTAQSLGVKPTEI
                     VHVGDALAADVEGALNVGMRVIWFAGDPDQRTRFGEDVRLAFNAADVREALRAWLGR"
     gene            complement(490..1206)
                     /locus_tag="VFQ61_31695"
     CDS             complement(490..1206)
                     /locus_tag="VFQ61_31695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959112.1"
                     /translation="MAHFPAFRLLCLSLAVLACESKGPPKPAPAAASASPSVSAASRS
                     QPAVIATAPRRAAGPMGPRLALLPGQGVGAIRIGATVPTIERLLGMACELKTESSCRY
                     IDLALEFTLENGATTAIRAYRPGRPAGKNAAGLESEYGYFNGAIPPDLMFGMLPQAIQ
                     EHLGQPQRVERRDVVGPADAVETHYYDGIVLEYDRIANGNLVLGSAQVVKGSGAPSDP
                     GAAKPKPGAAKPAAPPVPRK"
     gene            1364..2320
                     /locus_tag="VFQ61_31700"
     CDS             1364..2320
                     /locus_tag="VFQ61_31700"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HET9959113.1"
                     /translation="MQSLENRRFLFVTGKGGAGKTSVSAALALALSRAGSRVLVTTSG
                     ARDTLSPMLASAGLAGAGPRAAEAIELGTEIRRIAPNLWGVRLEANVAMREYGAMVLH
                     TPKLVNALFDNKYVEGLFSGAPGLKEWALLGKAWYHSTERLPNGEPRFNVIVFDAPAT
                     GHGLDMLRVPRVILSAAPEGRLRKDAERALNSLKDPAQSGVVVVSLPEEMPTNETLEL
                     LAALETDLGLPVAEIVLNSTLEQLFSDEEGRALEPFATYEGSDPAEAALGAAARRALS
                     ERAQRQSLERLSLTGVQVRALPRLDSSLSPAALASALSAYLP"
     gene            2449..2973
                     /locus_tag="VFQ61_31705"
     CDS             2449..2973
                     /locus_tag="VFQ61_31705"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET9959114.1"
                     /translation="MTAFQGKSGLDLDHELLESTISGTITGLQMADVVPRPVGASCLV
                     SARHTLSILVGMVGRHSGNMALNLSEVAVLYLAGRLLGEPQREINEDSIDAIMEIGNM
                     VAGAIKKPLRSSGYEIEQVSLPSLIMGQSYNMVYARGIRSISVEFDLPDLPFNSMTNR
                     FFTTSVSLLRGSGS"
     gene            complement(3020..4411)
                     /gene="asnS"
                     /locus_tag="VFQ61_31710"
     CDS             complement(3020..4411)
                     /gene="asnS"
                     /locus_tag="VFQ61_31710"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004244664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET9959115.1"
                     /translation="MQKPGTYVRDLLMREPPAAVTARGWIKTRRDSKTAHFIQLNDGS
                     CFRDLQVVFAEEQFRGVDLGELSTGACVEVDGELVVSPHALQRVELQARSFKVLGTAD
                     PLQYPLQKKGHTFEFLREIGHLRARSNTFGAVFRVRNVLSFAIHQFFQERGFLYLHAP
                     IITTSDCEGAGAMFQVTTLDLNQVPRTPENKPDYSRDFFGKPAYLTVSGQLEGEAFAL
                     AFSNIYTFGPTFRAENSNTPRHLAEFWMVEPEMAFCNLEQNMDLAEEFLKYIIEAVLD
                     RSAEDLEFFNQRIDNTVLATLEHVATSPFERITYTEAVKILESANKSWEFPVFFGADL
                     QSEHERYLTEEKFKRPVIVTDYPKQIKAFYMRQNEDGKTVRAMDVLAPRIGEIIGGSQ
                     REERSDVLKERLRAQNLSEEAYDWYLQLRAFGSAPHAGFGLGLERMLMYVTGMKNIRD
                     VIPFARTPGSAAF"
     gene            4620..4901
                     /locus_tag="VFQ61_31715"
     CDS             4620..4901
                     /locus_tag="VFQ61_31715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959116.1"
                     /translation="MTAEHRVWFYPLRRRFAFSSTAMFLALLAAPGWVAACGPNGGTY
                     CQSGPKYGTQCYAMPDVEHTPGAPPPMADPERPARGQSSAPNPKQWPRR"
     gene            5077..6084
                     /locus_tag="VFQ61_31720"
     CDS             5077..6084
                     /locus_tag="VFQ61_31720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9959117.1"
                     /translation="MTYKPDPKRYDATNLYRRCGKSGLMLPQISLGLWHNFGAKDDPE
                     RARGMLLRAFDLGITHFDLANNYGPPGGAAELTLGRIMYEDLAAYRDELIISTKAGYD
                     MWPGPYGNFGSRKYLLASLDQSLERLGLDYVDIFYSHRPDPETPIEETCGALAQAVRQ
                     GKALYVGISNYNPEQTEVALRVLRELGTPCLIHQPRYSMFDRTPEKSGLFEVLRREGT
                     GSIVFSPLAQGLLSSRYLTGLPKDARAVRDGRFLKPEHITKEKLDLLHALDGFAKERG
                     QTLAQLALSWVLREEPVTSALIGASSVAQIEENVAAAQGPTWKADELGRIEQLLAGSA
                     G"
     gene            6150..7649
                     /locus_tag="VFQ61_31725"
     CDS             6150..7649
                     /locus_tag="VFQ61_31725"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HET9959118.1"
                     /translation="MSYAVLEQHHRKLSHLKHVAAFTAWDEATNMPAGGGEARAEAMA
                     TLQGLIHEQSTRPELADWLAGAEAHVAELEPWQAANLREMKRAHQHASAVPQSLVEAT
                     TRATLRSEQAWRSLRAQNDWAAFRPLLEEVVTLKRESAQALSAALNVGLYDALLDEFE
                     PFARVSQIDPIFTRLREFLPSAVARATERSRSLTRIVPQGPFSTEVQRALGQQLMKRV
                     GFDFSHGRLDTSHHPFCGGVPQDVRITTRYDEADFAKALMGVLHETGHAKYEQNLPQA
                     WIDQPVGAARGMSMHESQSLLLEMQVCRSREFTRFVAPILAEAFASASAANPEAFTEE
                     NLYRLATRVEPGFIRVDADEVTYPCHILLRYEIERPLIEGKLEVKDIPESWDRGMQSL
                     LGLSTHGNDRDGCMQDVHWPAGLFGYFPTYTLGALTAAQLFRAARRAVPGLLEQIARG
                     EFGGLDGWLRTNVWGVGSSLSTNEILARATGSTLNTQAFEEHVARRYLG"
     gene            complement(7802..8767)
                     /locus_tag="VFQ61_31730"
     CDS             complement(7802..8767)
                     /locus_tag="VFQ61_31730"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET9959119.1"
                     /translation="MTFTQRVISAPAPIPRRAKSLREYQWAASVPFFLMHLLPLGALF
                     TGARLRDFVVCAVLYVVRMFGVTGAYHRYFAHRSYKTSRVFQFMLAFLAQTSAQKGAL
                     WWAAHHRTHHKYSDQDGDPHDSRRGFWYSHMGWILDDTNGTDYSKIKDLTKYPELVWL
                     NVIPNLPSALLAVTVWYFMGWSGLFIGFGLSTVLLYHGTFTINSLCHMIGKPRYASGD
                     LSKNSFLLAIITLGEGWHNNHHYFMNSTRQGFFWWELDITYYVLKALSWVGLVWDLKA
                     PPARAYDPKNFLAHGATLSSTQNENDSPGALTESALTGSVLPPAA"
     gene            complement(8962..9669)
                     /gene="pgl"
                     /locus_tag="VFQ61_31735"
     CDS             complement(8962..9669)
                     /gene="pgl"
                     /locus_tag="VFQ61_31735"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET9959120.1"
                     /translation="MSRRIERVRMAAPRIASYAAEEIAAALTRAVAQRGHASLCLSGG
                     TTPRATYQELAKLDHVPWALVSFFFGDERCVPPDHADSNFAMAKSALFDHVPLSPDRV
                     FRVRGEDPDPVASAQDYARVLPDALDVLVLGIGEDGHTASLFPGSEALLEREAKARFV
                     TGPKPPPRRITITPPVIAAARCIYMLASGEGKAEAVARALEGTTTPTECPALLARQGV
                     WLMDHAAGGRLTGVLTP"
     gene            9726..11288
                     /locus_tag="VFQ61_31740"
     CDS             9726..11288
                     /locus_tag="VFQ61_31740"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9959121.1"
                     /translation="MGSPAFEPQRVLSGRYRLEQRLGSGGMGAIWRAQHLTLEAPVAV
                     KLIEHDAVPDEQTVSRFLREAKAAAALRSRHVVQILDYGVEDGVPFIVMELLEGENLA
                     QRIKRLGRLSPGETVRIVTHVARAIGRAHDAGIVHRDLKPENVFLVPSDEGVDAKVLD
                     FGVAKVEKGVLRGDGTETRTGSILGTPYYMSPEQAQGNKTVDFRTDLWALAVIVFECL
                     TGTRPFYSDGLGDLVLQICVRDVPVPSQVAEVPPGFDAWFARATQREPEQRFQSAREL
                     AETLAEALNVEMRGSAPESQLPAFTSPEPPHSLNQTSARRRRPEPSTPDPTAPTMEVE
                     KLHVQTELEVTARPYESLQAPGSGLGMVVAVAAVALGLGLGGGIWLIQKMGKGASPWS
                     SAAPAASTQHAVRRSESPPTPVAPPAVTIQTAPSDEPLVPEGSARGQAPSANTGQPPS
                     SPSGGGAPVRPTPASRNNAEPRGPADAGVAPPDAWVKPAWAIPDEEPRRVDPIDENPA
                     KDEKNKPSEPPH"
     gene            complement(11354..12238)
                     /locus_tag="VFQ61_31745"
     CDS             complement(11354..12238)
                     /locus_tag="VFQ61_31745"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9959122.1"
                     /translation="MNWELARVFLSVARAGSMFAAARELGMSQPSVSRAVYDLERSVG
                     QKLFVRHARGVALTPVGEEARAAAERIELGMQSFARAARRKPEVEGTLRLATTEFIGV
                     EVLAPRLHELRELFPKLRLELVLQNTASDLARGEADLALRLFRPKQASLVTKQVAALE
                     LGLYASRAYLERKGEPRNFEELLDHELIGFDPKGPMSRLFTALDARLVGERFAIGTDC
                     LSAHVALARGGFGIAGLQRGFARNYQELCRILPALPLPAVEAWLVTHPDLRRAGQVRA
                     AWDWVEAVIREYAGETRR"
     gene            12326..13009
                     /locus_tag="VFQ61_31750"
     CDS             12326..13009
                     /locus_tag="VFQ61_31750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959123.1"
                     /translation="MLRLVMQSTFLQQIGIHRVLGPLVAGFTLTLGVPASADVPRESE
                     PPPPARASTRSKRYDETAQPKAAPRPGMWVDLEIDPIAYIAQGYSLHLGLRASHLRFD
                     LGTFGLLMPEFVHGQAGFENRASGYGIKLDYYVRPEQSGFFFGLEGGTIQQQIVDEHT
                     DTLVRVTSLAGGVRTGYEFPLPGGFYIKPWIGLGRRFGHEDVRVAGRNFHQSPWLVFP
                     TVHLGYAFP"
     gene            13144..14970
                     /locus_tag="VFQ61_31755"
     CDS             13144..14970
                     /locus_tag="VFQ61_31755"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HET9959124.1"
                     /translation="MDRASRVSMTTLPRAAKLLDRSEAVLLASSMGLLVGDFAAPPWW
                     GVSVVLLALTSLLGVAFSGLVLRSSAAPAPLDSAMQTRTLRERVFQVLILGTIWLLIG
                     AKWWVLVSYARSDPSQFVAFTRSYDVVLMMSLPLGVLVRRGRLTRILALIEAHPARLM
                     AASFGSIGLLGSLLLSLPISLQRVGELSFVDNLFMAFSAVCVTGLSVNTVSQTYTLFG
                     QLVLCLLIQVGGLSIMVLSAAIGLLAGGRMRVRSSVVFAGIVDAESISGLRRQALDIV
                     TATLLLEMAGGLLLYSRFSALSRSVSPGLTAAHGNPIAGPETPEWAAIFHAVSAFCNA
                     GFSNFETGLVSFADDPLVVLVTTALILLGGIGFPVIGELWPRLWRFVIRQRRERLSLN
                     TRVCLYTTAALFLGMAGAYLVLEWSASFAGLHPASRLLAAIFHSASCRTAGFNIVDVG
                     AMRPSTLMITCIAMFIGACPGSCAGGIKTTTLAVLFASMRAELRGRPAQLFDRLLPEA
                     TLRKAVGVVFTSIGIVALALFALLLIEPHEPLKLAFEVFSAFSTTGLSTGITPALSVP
                     GKLLITLVMYLGRIGPLTLALAFAARTDTSPLRLPSERVQIG"
     gene            15003..15677
                     /locus_tag="VFQ61_31760"
     CDS             15003..15677
                     /locus_tag="VFQ61_31760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET9959125.1"
                     /translation="MKGAKRVLVIGLGRFGSALVEELWDAGCELIVIDRQPEAVDRLK
                     TKAAAAFVGDATELAVLESLGAKEVDVAVVTAGEAFEGVVLCVSALAQMGVKVIFARA
                     ANDRQAHVLERVGATRAVQVENEMGRRLAIQVLNPVAAPLLDFATHFRVVPWTVPAAF
                     VGRTLAETAFRRHEINVLGWLSLEGSSKPRLQFPASDYRMAAGHTLLLAGEEEAIERF
                     LATTER"
     gene            complement(15919..16485)
                     /locus_tag="VFQ61_31765"
     CDS             complement(15919..16485)
                     /locus_tag="VFQ61_31765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959126.1"
                     /translation="MGLFSGSSSQGSPFPHGAPFQRSTGTLDRASRHQIWDLDTNHVQ
                     ATQGQVFYTYSKGEASSSNIRSMVNNLDAFARETKLPIGYVLHLVSSAKPPASEDREL
                     TTAMFNRHAGRITGLAVVIEADGFTGAALRSAVTMVFMMTCRGFPTRTFPNIRAAADW
                     LAERQRVPAAEIIRLSEEAHGALRRAGT"
     gene            complement(16493..17935)
                     /locus_tag="VFQ61_31770"
     CDS             complement(16493..17935)
                     /locus_tag="VFQ61_31770"
                     /inference="COORDINATES: protein motif:HMM:NF020826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET9959127.1"
                     /translation="MNLSRRQLFAGAASAVAVYGFDPVAGIWVSEARAGCIRAPDLRG
                     ELVFESTAIAEAAEDFGHIEHRLSWAVLRPGNFEDVVRMVRFANQQGLPIAMRGQGHS
                     VLGQAQAPSGIVIDSRSLNRIHRLDADGAIVETGVTWSELVTRATAVGLTPPVLTDYL
                     HLSIGGVLSVGGVGGSSHRHGLVVDQVEELTVVTGQGELVTCSSRHHRVLFESTLGGL
                     GQFALILQAKLRLRAAHAMARVYQLVYTDLSRYLRAHRALDGTKTLDFLQGLVSPGPE
                     GGWQYILQCAQWYDSTPPPSPLDPLSADLAPLATEISDLPYLAWTSRVDQFVAGWASA
                     GVWTTPHPWSDLFLPDDAVEAFVTKALANTTPADVGAGVILLYPLRRGGAALPFLRTP
                     AGDRIWLFDILRFAPSDPNIVQGMLNANRALLDQAVAIGGKRYPISAVRMRPADWVAH
                     YDSAYPALVIRKAAFDPRNVLTPGQRIFRQ"
     gene            complement(18004..18909)
                     /locus_tag="VFQ61_31775"
     CDS             complement(18004..18909)
                     /locus_tag="VFQ61_31775"
                     /inference="COORDINATES: protein motif:HMM:NF022907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic prenyltransferase"
                     /protein_id="HET9959128.1"
                     /translation="MNATILSAAQSRASARLMNDLVTCARHAGVIADRAKIAEGLALF
                     ADRFASKPVEIRTTSQPLAKREVSFRFVDEDARGEVWNIASDWYEIEGEPRAFMDAIT
                     SAFGIRAEGLDCDVRGGFRKVWAFLDQGFRSERFERLPGAPPALVRVRSVLERYGLQH
                     MSIVGIDPANRTCNLYPILRPGWATPEVVASVARELSFNGMAEGWLQHIERSVAANFT
                     FAWDSERVERLAFYRPAMSPEELPDDELLRQFALRCPTVATQRAYIPSVAYASSGHYR
                     KVEVDYDGGIVPVLVRCAQVPNEAP"
     gene            20010..>20534
                     /locus_tag="VFQ61_31780"
     CDS             20010..>20534
                     /locus_tag="VFQ61_31780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959129.1"
                     /translation="MRFIVMHKVDEKMEAGAPPNQEIISAMGSLVRESLSNGVFQNGA
                     GLHRSAQRVRLSFRDGRCTVTQGPYQGQNELVWAFAMIQTPAMQGAIEQASRYADLLG
                     DSEIEIGPVVEAWDLGIVPKPAHIEKSRFLLLIKGNAATEGNRLDKAMLQRLGELEQQ
                     LTTEGVLLAAEKLAP"
BASE COUNT         3747 a         6331 c         6638 g         3818 t
ORIGIN      
        1 gggctggtac gccgaaagag cgcattcacg agaccgtgga gtggctctgg caggagcaac
       61 ccaaatacaa tctttggcgc cgcccgatcg cgggcatgtt cgagctggtg cgcgagctgc
      121 acgacatgca gattccactc ggcatcatct cgaactcgga gggccgcttg gccgagctgg
      181 cggatgagct ggggattggg cagtacttcc gggtggtggc cgattctggg aagttcggga
      241 ttccaaaacc aaaagcggaa atcttcctgt ataccgcgca gtcgctcggc gtgaaaccca
      301 ccgagatcgt gcacgtggga gacgcactcg cggcggatgt cgagggagcc ttgaacgtcg
      361 gcatgcgggt gatttggttc gcgggcgatc ccgatcaacg cacccgtttc ggggaagacg
      421 tgcgcctcgc attcaatgcc gcagacgtgc gcgaggctct gcgcgcgtgg ctcgggcggt
      481 gaaaaagcct tatttccgcg ggacaggcgg cgcagcaggc ttggcggcgc ccggcttcgg
      541 tttcgccgct cccgggtccg atggtgcgcc agagcctttg acgacctgag cgcttccgag
      601 cactagattc ccgttggcga tgcggtcgta ctcgagcacg atgccatcgt aatagtgggt
      661 ctctactgcg tcggcaggac caacgacgtc acgacgttcg acacgttgcg gttgccccag
      721 gtgctcttga atcgcctgcg ggagcatgcc gaacatgaga tcgggaggaa tcgcgccgtt
      781 gaagtagccg tactcggact cgagcccggc cgcgttcttt ccggcggggc gccccggacg
      841 ataggcgcgt atcgccgtcg tggctccatt ttccaacgtg aactccaggg ccaagtcgat
      901 ataccggcag gagctctcgg tcttcaactc gcaggccata cccagcagcc gctcgatggt
      961 tggcacggtc gccccgatgc gaatggcgcc gacaccttgg ccgggcagga gggccaagcg
     1021 cggccccatc gggccggcgg ctcgacgtgg tgccgtggcg atcacggccg gctgtgaccg
     1081 agatgctgct gaaacggagg gcgaggccga tgcggccgcg ggagcgggct tggggggacc
     1141 tttggattca caggccaaaa ccgcgagtga aaggcacagg agccgaaaag ccgggaaatg
     1201 agccatttga ttttcgtagc gggccgagac atgtaccacg cgacgcgctc aatcgtcacg
     1261 cccaggcccc gctcgatatt cccaacctcc accgcgccaa caaccaccgg caaagcacct
     1321 cgcgttccga cgagcccgcc tcagtcctca tctagcagcc gccatgcaat ctctggaaaa
     1381 ccgacggttc ctcttcgtga cgggcaaggg cggcgcgggc aaaacctcgg tctcggcggc
     1441 gctggcgctg gctctatccc gggcgggatc tcgcgttctg gtcaccacca gcggggcgcg
     1501 ggacacactc tcgccgatgc tggcaagtgc gggattggct ggggcagggc ctcgcgccgc
     1561 cgaagcgata gagctcggca ccgagatccg taggattgcg ccgaatcttt ggggggttcg
     1621 cctcgaggcc aacgtggcca tgcgcgaata cggcgcgatg gttctgcata cgccgaagct
     1681 cgtcaatgcc ttgttcgata acaagtacgt cgaagggcta ttcagcggcg ctcccggact
     1741 caaagagtgg gcgctgctcg gcaaggcttg gtatcactcg accgaacgtc tgccgaacgg
     1801 cgagcctcgc ttcaacgtga ttgtgttcga tgcgccagcg accgggcacg gcctcgacat
     1861 gctgcgggtg ccgcgggtga tcttgtcggc ggcgcccgag gggcgtctac gcaaggacgc
     1921 ggagcgcgcg ctgaattcgc tgaaggatcc cgcgcagagt ggggtagtcg tggtgtccct
     1981 gcccgaagag atgccgacca acgagacatt ggagctgctc gcggccctgg agaccgactt
     2041 gggcttaccc gtggcggaga tcgtgttgaa ctccacactc gagcagctgt tcagtgacga
     2101 agagggacgc gccctcgagc ccttcgccac ctacgaaggc agcgatcccg cggaagccgc
     2161 gctgggcgcc gcggcgcgcc gggccttgtc cgagcgagcg caacgccaaa gtctagagcg
     2221 actctcgctg acaggtgttc aggtccgggc tctgccgcgc ttggactcca gcttgtcgcc
     2281 ggccgcgctc gccagtgcgt tgagtgcgta cttgccctga ctgatcgcgc ggatacgccg
     2341 ccagttttgc cacaagtttt gcaccggttt cacggtcgcg ggctaacctc atgcccaccc
     2401 tggacgctag tgccaagtag ggtcttccgc taacggccct tcttccccat gacggcattt
     2461 cagggaaaat ccgggctcga tttggatcac gagctgctcg aaagtacgat ttcgggcacc
     2521 atcactggtt tgcaaatggc agacgtggtg ccccgaccgg tgggggccag ctgtttggtg
     2581 tccgcgcgac acacgttgtc gattctggtg ggcatggtgg ggcgccacag tgggaatatg
     2641 gcgctgaacc tctcggaagt cgcggtgctg tatctcgcgg ggcgcttgct cggcgaaccg
     2701 cagcgcgaga tcaacgaaga cagcatcgac gcaatcatgg agattggcaa tatggtggcc
     2761 ggtgcgatca agaaaccgct gcgctccagc ggctacgaga ttgaacaggt ttcgttgccg
     2821 tccctgatca tgggccagag ttacaacatg gtgtacgcgc gcggcatccg aagcatctcc
     2881 gtcgagttcg atttgccgga tttgcctttc aacagcatga ccaatcgctt cttcaccacc
     2941 agcgtgtccc tgctgcgcgg ttcaggtagc tgagagaccg actctctgct agggctgcgc
     3001 gcggcgcgca tgctcgccct caaaacgcgg ccgagccggg ggtgcgtgcg aacgggatca
     3061 cgtcccggat gttcttcatg ccggtcacgt acatgagcat ccgctcgagc ccgagcccga
     3121 agccggcgtg cggggcggag ccgaacgcac ggagctgcag ataccaatcg taggcttcct
     3181 cgctcaggtt ctgcgcgcgc aaacgctcct tcagcacgtc gctgcgctct tcccgttggc
     3241 tgccgccgat gatctcgccg atgcgcgggg ccaggacgtc catggcgcgc acggtcttgc
     3301 cgtcctcgtt ttgcctcatg tagaaggctt tgatctgttt ggggtagtcg gtgacgatga
     3361 cgggacgctt gaacttctct tcggtcaaat agcgctcgtg ctcgctctgc aggtcggcgc
     3421 cgaagaacac cggaaattcc cagctcttgt tggcagactc caggatcttc accgcttcgg
     3481 tataggtgat gcgctcgaac ggagaggtgg ctacgtgctc gagcgtggcg agtacggtgt
     3541 tgtcgatgcg ctgattgaag aactcgagat cttcggccga gcgatcgagc accgcctcga
     3601 tgatatactt caggaactcc tcggcgagat ccatgttctg ctccaagttg cagaacgcca
     3661 tttccggttc caccatccaa aactcggcca ggtgccgcgg tgtattggag ttctcggcgc
     3721 ggaacgtggg tccgaaggtg tagatgttgc tgaaggcgag cgcgaacgct tcgccttcta
     3781 gttgcccgct taccgtcaga tacgccggtt tgccgaagaa atcgcgactg tagtcgggct
     3841 tgttctccgg cgtgcgcggc acctggttca aatccagcgt ggtgacttgg aacatggcgc
     3901 ctgcgccctc gcagtccgag gtggtgatga tgggcgcgtg cagatacaag aagccgcgtt
     3961 cctggaagaa ctgatgaatc gcgaagctca gcacgttgcg cacgcggaac acggcgccaa
     4021 aagtgttgga ccgcgcgcgc aggtggccaa tctcacgcaa aaactcgaag gtgtggccct
     4081 tcttttgtag tggatactgc agcgggtcag ccgtgccgag cactttgaag ctacgtgcct
     4141 gcagctccac gcgctgcagg gcgtgcgggg agaccacgag ttcgccgtcg acctcgacgc
     4201 aggcgcccgt ggagagctcg ccgaggtcga cgccgcggaa ctgctcctcg gcaaacacga
     4261 cctggaggtc tcgaaagcag ctgccgtcat tgagttggat gaagtgggcc gtcttgctgt
     4321 cgcggcgggt tttgatccag ccgcgtgccg tgacggcggc agggggctca cgcatgagta
     4381 ggtcgcgaac gtacgtaccc ggcttttgca tcgcacccga gcatagccga gtcgcccgcg
     4441 gctgtgacct gccatgccga gcgcggccac taacccggac catacgccct gtgcccgggc
     4501 ttcacgcctg acgctgccaa agccggttca cggtgcgtta tcagccgccg atttttagct
     4561 cattttgtgc aggtggcggc ttgcgacgcc cgggcacacg gcttgcccaa tcggtcggta
     4621 tgactgccga acaccgggtt tggttctatc ccttgcggcg caggttcgcg ttctcgagca
     4681 cggccatgtt tttggcgctg ctggcagcac cgggatgggt ggccgcgtgt gggcccaacg
     4741 gcggcacgta ttgtcagagc gggcccaagt acggcactca gtgttacgcg atgccggacg
     4801 tggagcacac tccgggtgcg ccgccgccca tggccgatcc ggagcggccc gcccggggcc
     4861 aaagctcggc accaaacccc aagcaatggc cacgccggtg agcggttttg cgcggctggt
     4921 ccgtgtttct cgaacaacgc atgtcgagag ctgctttcaa gccgagtgag ccgaaatgcg
     4981 tggcacgagt gccgggctcg cgcggcttgc ggcgccggct tccctccgcc atgctcggcg
     5041 ccctcactga ccagcacgtt ctggctaggc aacgacatga cgtacaagcc cgatccgaag
     5101 cgctacgacg cgacgaacct ctaccgccgc tgcggcaaga gcggcctcat gctgccgcag
     5161 atctcgctgg ggctgtggca caacttcggc gccaaggatg acccggagcg cgcgcgtggc
     5221 atgttgctcc gagccttcga cttgggcatt acccatttcg atctggcgaa caactacggc
     5281 ccgcccggcg gtgccgccga gctgacgctg ggccgcatca tgtacgaaga tttggcggcg
     5341 tatcgcgacg agctcatcat ctcgacgaag gcgggatacg atatgtggcc ggggccctat
     5401 ggtaacttcg gttcgcgcaa gtacttgctc gcgagcttgg accagagcct cgagcgcttg
     5461 gggctcgact atgtggacat cttctacagt catcgccccg atcccgagac cccgatcgag
     5521 gaaacgtgcg gtgcgctggc tcaggcggtg cgccagggta aggcgctcta tgtcggaatc
     5581 tcgaactata accccgagca aaccgaagta gcgctcaggg ttctccgcga gctcgggacg
     5641 ccctgtctaa tccaccaacc tcgctacagc atgttcgacc gtacaccgga gaagagcggc
     5701 ctgttcgagg tgctgcggcg tgaaggcacc ggtagcatcg tgttttcccc gctcgcgcag
     5761 ggcctgctct cgagccgcta tttgacgggg ttacccaaag atgcgcgcgc cgtacgcgat
     5821 gggcgcttct tgaaacccga gcacatcacc aaagagaagc tcgatttgct ccacgctttg
     5881 gacggctttg ccaaagagcg agggcagacg ctcgcgcagc tcgcgttgtc ttgggtatta
     5941 cgggaggagc cggtcacctc ggcgttgatc ggcgcgagct cggtcgccca aatcgaggag
     6001 aacgtcgcgg cagcccaagg acctacgtgg aaggctgacg aactcgggcg gatcgagcag
     6061 ctgctggcgg gttctgccgg ttgagaaccg agcgaggtcg cgaggagctg gcctgagccc
     6121 aagctccagg atcgtgtaag ctctcgggca tgtcctacgc ggtcttggag caacaccatc
     6181 gaaagctgtc gcacctcaag cacgtcgccg cgttcacggc ttgggatgag gcgaccaaca
     6241 tgccggcggg cggcggcgag gcacgcgcgg aggcgatggc cacgctgcag ggcctaatcc
     6301 acgagcaaag cacgcgccct gagctggcag actggctcgc cggcgcggaa gcccacgtgg
     6361 ccgagctcga gccgtggcag gccgcgaatt tgcgggaaat gaagcgcgct catcaacacg
     6421 cgagcgctgt gcctcagtcc ttggtggagg ccacgacgcg ggccacgctt cgcagcgagc
     6481 aagcctggcg ttcgctccga gctcagaacg actgggcggc gtttcgcccg ctgctcgagg
     6541 aggtggtgac gctcaagcga gagtcggcgc aggccttgtc ggcggcgctg aatgtggggc
     6601 tctacgacgc gttgctggac gagttcgagc cgtttgcgcg tgtctcgcag atcgatccga
     6661 tcttcaccag actgcgtgag ttcttgccga gcgcggtggc acgagccacc gaacgctcac
     6721 gatcgcttac gaggatcgtg ccacagggac cgttcagtac ggaggtgcag cgcgccctgg
     6781 ggcaacagct gatgaaacgg gtcggcttcg acttctcgca tggtcgtttg gacaccagcc
     6841 accacccgtt ctgcggcggg gtgccgcaag acgtgcggat tactactcgc tacgacgaag
     6901 ccgatttcgc caaagccctg atgggtgtgc tgcacgaaac cggacacgcc aaatacgagc
     6961 agaacttgcc ccaagcttgg atcgatcaac cggtcggcgc ggctcgtggc atgagcatgc
     7021 acgagagcca gagcttactg ctcgaaatgc aggtctgccg gagtcgcgag tttacgcgct
     7081 tcgtggcccc gatcctggcg gaggcctttg cgtcggcgag cgcggccaac ccggaagcat
     7141 tcacggagga aaatctctat cgcctggcca cacgcgtgga gccgggcttc attcgcgtgg
     7201 acgccgacga ggtgacctat ccctgccata tcttgttgcg ctacgagatc gaacggccgc
     7261 tgatcgaagg caaactcgag gtcaaagaca ttcccgagtc gtgggatcga ggaatgcaat
     7321 ccctgcttgg cttgagcacc cacggcaacg atcgggacgg ctgcatgcaa gacgttcact
     7381 ggccggcagg gctgttcggg tatttcccaa cgtacacgct cggagccctc accgcagctc
     7441 agctctttcg cgcggcgcga cgagctgtgc ccgggctttt ggagcagatt gcgcgcggtg
     7501 agttcggcgg cctcgacggt tggctacgaa cgaacgtctg gggcgtcggc tcgtcgctca
     7561 gcaccaacga gatattggcc cgcgccacgg gcagcacttt gaacacgcag gccttcgaag
     7621 agcacgtggc gcgtcgctac ttaggctgaa attcgcggct gaatgcgaag ccgtaggcgg
     7681 agaaccgaag gcttcgagcg ctgctcccca acgaagtggg ccgctcacaa aggaaaagag
     7741 gcgaccagtt aggtcgcctc tttactctac cgagctcttc acctgcagag cagagcgagc
     7801 gctacgccgc gggtggcagc accgagcccg tcagtgccga ctcggtcaac gctcccgggc
     7861 tgtcattctc gttttgggtg gacgagagcg ttgcgccgtg ggccaggaag ttcttggggt
     7921 cataagctcg ggccggcggc gccttcaaat cccaaaccaa acctacccaa gacagcgcct
     7981 tgagcacgta atacgtaata tcgagttccc accagaagaa accctgacgg gtggagttca
     8041 tgaaataatg gtggttgttg tgccaacctt caccgagcgt aataatcgcg agcaggaagc
     8101 tgtttttgct caggtcaccg gaagcgtagc gcggcttgcc gatcatgtga cacagcgaat
     8161 tgatggtgaa ggtgccgtga tagagcagca ccgtgctgag cccgaagccg atgaataggc
     8221 cggaccaacc catgaagtac caaaccgtta ccgcgagcag cgcgctcggc agattgggaa
     8281 tgacgttcaa ccacacgagc tccggatact tcgtgagatc tttgatcttc gagtagtccg
     8341 tgccgttcgt gtcatccaag atccaaccca tgtgcgagta ccagaacccg cggcgcgaat
     8401 cgtggggatc gccgtcttgg tccgagtact tgtggtgcgt gcggtgatgc gcggcccacc
     8461 acagcgcgcc tttctgcgcg ctggtttgcg cgaggaaggc caacatgaat tggaaaacgc
     8521 ggctggtctt gtagctacga tgcgcaaagt accgatggta agcgcccgtg acgccgaaca
     8581 tgcgcaccac gtagagcacc gcgcacacga cgaagtcgcg taggcgcgca ccggtgaaga
     8641 gcgcccccag cgggagtaga tgcatcagga agaacggcac cgacgccgcc cactgatact
     8701 ctcgcaaaga cttcgcgcgg cgggggatcg gagcgggggc cgagataaca cgttgggtga
     8761 aggtcactgc ttgggaatgt tccatcgaac cgcgcgcaaa acagtcacga ttgtgtcatg
     8821 tcgtcatgac aatcggcgtg aactcctggg ccgttcgtca ggcctttgtc atgcgcgccc
     8881 gaaagataac cccccgcacg aaacaataac ctactactgt ggagcgacgt agtcgcatca
     8941 cccaactcga ccgttggcgt gtcacggcgt gagcacgccg gtaaggcgtc cacccgccgc
     9001 atgatccatg agccatacac cctggcgcgc gagcagggcc gggcattccg ttggcgtcgt
     9061 cgtgccctcg agcgctcgcg cgacggcttc cgccttgccc tctcctgagg ccaacatgta
     9121 gatgcagcgt gcggccgcga tcacaggggg cgtgatggtg atgcgacgcg ggggcggctt
     9181 ggggccggtc acgaagcggg ccttggcctc tcgttccagc agggcttcgg agccgggaaa
     9241 cagcgacgcc gtatgcccgt cctctccgat cccaaggacg agcacgtcga gcgcatccgg
     9301 cagtacacgt gcgtagtctt gcgcactcgc cacagggtcc ggatcctcgc cgcgaacccg
     9361 aaacactcga tcgggcgaca gcggcacgtg gtcgaacagc gcgcttttcg ccatcgcaaa
     9421 gttactgtct gcgtgatctg gggggacgca gcgctcgtcc ccaaaaaaga acgagaccag
     9481 agcccagggc acatgatcga gctttgcaag ctcttggtac gtggctcgcg gtgtggttcc
     9541 gccggaaagg caaagggagg cgtggccccg ctgggccacg gctcgcgtga gggcggccgc
     9601 gatttcttcg gccgcgtagg aggcaattct cggcgcggcc atccgcaccc gctcgattct
     9661 tcggctcatg atgcgcgaga tttaatgcat ggcgaggggg acggcgctat ggtcttgagc
     9721 ccggaatggg aagccccgcg ttcgaacccc agcgtgtact atccggtcgg tatcggctgg
     9781 aacagcgcct cggttccggt ggcatgggcg cgatttggcg cgcccagcac cttacgctcg
     9841 aggctccagt cgcggtcaag ctgatcgagc acgatgcggt gccggatgag cagacggtct
     9901 ctcgtttcct gcgtgaggcc aaagctgcgg cggccctgcg aagtcgccac gtcgtccaga
     9961 tcttggacta cggcgtggaa gatggcgtgc cgttcatcgt catggagctg ctcgagggtg
    10021 agaacctcgc tcagcgcatc aaacggctcg gacggctgag cccgggggag accgttcgca
    10081 tcgtcaccca cgtggcgcgg gccatcggac gcgctcacga tgcgggcatc gtgcaccgag
    10141 atctcaagcc cgagaacgtg ttcttggtgc cgagtgacga aggtgtggac gccaaggtct
    10201 tggatttcgg cgtcgccaag gtcgagaaag gcgtgctccg gggtgacggc acggagacgc
    10261 gcacgggctc aatcctcggc acgccgtatt acatgagtcc ggagcaagcg cagggcaaca
    10321 agaccgtgga ttttcgcacg gatctatggg ctctcgcggt gatcgtgttc gagtgcctga
    10381 ccggcacgag gccgttttac agcgacggtc tcggcgatct cgtgctgcag atttgcgtac
    10441 gagatgtgcc cgtgccgagc caagttgccg aagtgccacc cggcttcgac gcctggtttg
    10501 cccgtgctac ccagcgcgag ccggaacagc gcttccaatc cgcgcgagag ctcgcggaga
    10561 cgctggccga agcgttgaac gtagaaatgc gcggctctgc tcccgagagc cagctgcccg
    10621 ccttcacctc gcccgagccg ccgcatagcc tcaaccaaac ctccgcgcgt cggcgccggc
    10681 ccgagccgag cacgcccgat cccactgcgc cgacgatgga ggtcgaaaag ctccatgtgc
    10741 aaacggagct cgaagtgacg gcgcggccgt acgagagctt acaggcgccg ggctccgggc
    10801 tgggcatggt cgtggcggtg gccgcggttg cgctcggatt gggccttggc ggcggcattt
    10861 ggctcattca gaagatggga aagggcgcgt ccccgtggtc ctcagcggcg cctgccgcca
    10921 gcacgcaaca cgcggttagg cgttcggagt cacccccaac cccagtcgct ccgccagccg
    10981 tgacgattca gaccgcgccg agcgacgagc cgttggtccc cgagggcagc gcgcgcgggc
    11041 aggctcctag cgcgaacacc ggacaaccgc cgagctcgcc gagtggcggg ggagctccgg
    11101 tgcgcccaac tccagcgtcg cgcaacaacg cggagccgcg cggccctgcg gatgctggcg
    11161 tggcgccgcc ggacgcgtgg gtaaaaccgg cctgggcgat tcccgatgaa gaaccccggc
    11221 gagtcgatcc catcgatgaa aacccggcaa aagacgagaa gaacaagccg agtgagccgc
    11281 cccactgagt tattgggtag ccaggggtaa ccacggcgtg gcgctgccgc tcctcaaaac
    11341 tcgagtcttg agtttagcgc cgagtttcgc ctgcgtattc tcgaatcacg gcctctaccc
    11401 agtcccacgc ggcgcgcacc tggccagcgc gtcgcaaatc aggatgggtg acgagccaag
    11461 cctccaccgc gggcaacggc agcgccggta aaatccggca aagctcctga tagttgcgcg
    11521 caaacccgcg ttgcagcccg gcgatcccga acccaccgcg agcgagcgcg acgtgggccg
    11581 acaggcaatc cgtgccgatc gcgaagcgct cacccacgag gcgcgcatcg agcgccgtaa
    11641 acagccggct catcgggccc ttgggatcga acccgatcaa ctcatgatcg agcagctcct
    11701 cgaaattccg cggctcaccc ttcctttcga ggtacgcgcg ggaggcgtac aaacccagct
    11761 cgagggctgc gacttgcttc gtcacgagcg acgcttgttt gggacgaaaa agccgtaagg
    11821 ccagatcggc ctcgccacgc gccagatcgc tcgccgtatt ttgcagcacg agctccaagc
    11881 gcagtttggg gaagagctct cgtagctcgt gcaggcgcgg agccagaact tccacaccga
    11941 tgaactcagt ggtggctaga cgcaaagtcc cttccacttc gggtttacgc cgggccgcgc
    12001 gcgcgaagct ctgcatcccg agctcgattc gttcggccgc ggctcgtgcc tcttccccga
    12061 ccggggtgag cgccacgccg cgcgcgtgac gaacgaatag cttttggccc acgcttcgct
    12121 cgaggtcgta tacggcgcgg cttacggacg gctgactcat accgagctcg cgagcggccg
    12181 cgaacatcga gcccgctcgg gcgacgctca agaacacacg tgcgagctcc cagttcatcg
    12241 ctgggtctat acgaacggtc gagctattca gcaatgaata actgacggca aatctgagca
    12301 attcttctgc cggtaagaat agctcatgct ccgactcgtc atgcagagca cattccttca
    12361 gcaaatcggg attcatcgcg tactcggccc cttggtagcg gggttcactc tgacgctcgg
    12421 cgttccagct tccgccgatg tgccgcgtga gagcgagcct cctccgcccg ctcgggcgtc
    12481 gacgcggagc aaacgttacg acgagacggc tcagccaaaa gcggcgcccc gccctggcat
    12541 gtgggtcgat ctcgagatcg atccgattgc ctatatcgcg cagggatact cgctgcactt
    12601 ggggttgcgc gcctcccacc tgcgcttcga tctcggcacc ttcgggttgt tgatgcccga
    12661 gttcgtgcac ggccaggcgg gcttcgagaa tcgcgcgagc ggatacggca tcaagctcga
    12721 ttactatgtc cggcccgagc aaagtggctt cttcttcgga ttggaaggcg gcacgatcca
    12781 gcagcaaatc gtcgacgaac acaccgacac gctcgtacgc gtcaccagcc tggcgggcgg
    12841 tgttcgcacc ggttacgagt tcccgctgcc tggaggcttc tatatcaagc cttggattgg
    12901 gctcggccgc cgcttcggcc acgaagacgt gagggttgcc ggacgaaact ttcaccagag
    12961 cccctggctc gtcttcccca ccgtgcactt gggatacgca ttcccatgac gcgagtaggg
    13021 cctcggcact cgagtcttgg ggcccaggaa ggggcgcacc gcgcgggcgg ctcaatctcc
    13081 gcgtcagcag tgcccggggc tgacagcatg cccgatatct tatattgagg tgctaagtcc
    13141 gccatggata gagcttcccg cgtgtcgatg acgaccttgc ctcgggcggc gaagctcctg
    13201 gaccgctctg aagcggtgct gctcgccagc tcgatggggc tcttggtggg agacttcgcg
    13261 gctccgccct ggtggggcgt gtcggtcgtg ctgctcgcgt tgacctcgct gctcggggtt
    13321 gccttcagcg gtctcgtctt gcggagcagc gcggctccgg cgcctctaga ctcggctatg
    13381 cagacccgca cgctgagaga gcgcgtgttt caagtgctga ttctaggcac gatctggctc
    13441 ttgattggcg cgaagtggtg ggttctcgtg agctacgcgc gctcggaccc gtcgcagttc
    13501 gtcgcgttca cccgcagcta cgatgtcgtg ctgatgatga gcctgccgct cggggtgctg
    13561 gtgcgccgcg ggcggttgac ccggattttg gcgctgatcg aggcgcatcc ggcccgcttg
    13621 atggcggcgt cgtttgggag catcgggttg ctgggcagcc tccttctttc gttgccgatc
    13681 tcgctgcagc gagtcggtga gctgtcgttc gtggacaacc tgttcatggc cttcagcgcg
    13741 gtgtgtgtca ccgggctctc cgtcaataca gtgtcccaga cgtatacgct gttcgggcag
    13801 ctggtgctgt gtttgttgat ccaggtcggc gggctctcga tcatggtgtt gtcggccgcc
    13861 atcgggctgc tcgctggcgg gcgtatgcgt gtccgcagtt cggtggtgtt tgcagggatc
    13921 gtggacgccg agtcgatctc gggcctgcgc cgtcaggcgc tggacatcgt gaccgcgacg
    13981 ttgctgctcg aaatggccgg tggcttattg ctctattcgc ggttcagcgc gctttcgcgc
    14041 tcggtgtcac cgggtttgac cgccgctcat ggcaacccca tcgctggccc ggaaacgcca
    14101 gagtgggcgg cgatctttca cgcggtcagc gcgttttgta acgcaggatt ctcgaacttc
    14161 gagacgggct tggtctcctt cgccgacgac ccgctggtcg tgctcgtgac caccgctttg
    14221 attctgctcg gtggcattgg ctttccggtg attggcgagc tgtggccgag gctatggagg
    14281 ttcgtgattc gtcagcgtcg cgagcgtctg agcttgaaca ctcgggtttg cctgtacacc
    14341 acggccgctc tgttcctcgg tatggcgggg gcttatctag tgctcgaatg gagcgcctca
    14401 ttcgcggggc tccaccccgc gtcgcgcttg ctcgccgcca tctttcactc ggcttcttgc
    14461 cgtacggcgg ggttcaacat cgtggacgtg ggcgccatgc ggccttctac gctcatgatc
    14521 acttgcattg ccatgttcat tggcgcatgt cctggatcgt gcgcgggcgg gatcaagacc
    14581 acgactttgg cggtgctgtt cgcgagcatg cgcgccgaac tccgagggcg gccggcgcag
    14641 ctcttcgatc ggctgttgcc agaggccacc ttgcgcaagg cggttggcgt ggtgttcacc
    14701 agtatcggca tcgtagcgct ggcgctgttc gccctgctct tgatcgagcc gcacgagccc
    14761 ttgaagctcg cgttcgaggt gttcagcgcg ttctctacca cggggctgtc gaccggcatt
    14821 acgcctgccc tcagcgtgcc tgggaaactg ctgatcacgc tcgtcatgta cctgggtcga
    14881 attgggccgc tcacgttggc gttggcgttc gcggcacgca ccgatacgag cccgctacgc
    14941 ctcccaagtg agcgagtgca gattggttga gggccttgcc ccagtgagat aggttgaatg
    15001 caatgaaggg tgcaaagcgc gtattggtga ttggcctcgg ccggttcggc tcggcactcg
    15061 tggaagagtt gtgggacgcg ggttgcgagc tgatcgtcat cgatcgtcag cccgaggcgg
    15121 tggatcgcct gaagaccaag gcggccgccg cgttcgtggg ggacgccacc gagctcgccg
    15181 tactggagag tctgggggcc aaggaggtcg acgtcgcggt cgtgactgcg ggcgaagcgt
    15241 tcgaaggcgt ggtgctgtgc gtctcggcgc tcgcgcagat gggggtgaag gtgatcttcg
    15301 cccgcgccgc caatgaccga caggcgcacg tgctggagcg agtgggggcc acgcgtgcgg
    15361 tgcaagtcga gaacgagatg ggccggcgcc tcgcgattca agtgctcaac cccgtcgccg
    15421 ctccgcttct ggacttcgcc actcattttc gagtggtgcc ttggacggtg ccggccgcct
    15481 tcgtgggccg caccctcgcg gaaacggcct ttcggcgtca cgaaatcaac gtgctcggct
    15541 ggctatcgct cgaaggatcg tcgaagccac ggcttcagtt cccggccagt gattatcgta
    15601 tggccgcggg ccacacatta cttctcgccg gcgaagagga ggccatcgaa cgcttcctcg
    15661 ccacgacgga gcggtagcga cgctcgagag accgcatcag cagcgcagct tcgagcccgg
    15721 aagccgcgtc gaattgggcc ttggtttctg catagagttg ctcgagcgcg gcgagctctg
    15781 cctccgttgc cggagagcga aaggctcggc ccgcaaagcc ctcgatgaag cttcggtctt
    15841 cttcgcagat cggctctggt tagtaagagt ggttgcagcc gctcgcggga cgagcggcga
    15901 gtcccatcta gccgaagtct aggttccagc gcggcgcagg gcaccatgcg cctcttcgct
    15961 gagccgaatg atctccgcag cgggcacgcg ctgcctctcc gcgagccaat cggcggccgc
    16021 acgtatattc ggaaaggtgc gcgtcgggaa accgcggcaa gtcatcatga acaccatcgt
    16081 gaccgcgctg cgcaacgccg ctccggtgaa gccatcggct tcgatcacca ccgccaagcc
    16141 ggtgatacga cccgcgtgcc gattgaacat cgcagtcgtc aactctcgat cttcactcgc
    16201 gggcggcttg gcggaggaga ccaaatgcag cacatatcca atcggcaact tggtttcccg
    16261 cgcgaatgcg tccaggttgt tgaccatgct gcggatattg ctgctgctgg cttcgccttt
    16321 cgaatacgta tagaagactt ggccctgcgt ggcttgcacg tggttcgtgt ccagatccca
    16381 gatttggtgg cgggaggcgc ggtcgagcgt gccggtgcta cgttgaaacg gagcgccgtg
    16441 cggaaacgga gaaccctggc tggacgaacc tgagaacaag cccatgatgc ctctactgtc
    16501 ggaatatccg ctgtcctggc gtcaggacgt tgcggggatc gaacgcggct ttgcggatca
    16561 ccagcgcggg gtatgccgag tcgtagtgcg ccacccaatc cgcgggtcgc atgcgcaccg
    16621 ccgaaatcgg gtagcgcttg ccaccgatgg caacggcctg gtcgaggaga gctcgattcg
    16681 cgttcagcat gccctgcacg atgttcggat cagacggggc aaagcgcaag atgtcgaaca
    16741 accaaattcg atctccagct ggtgtacgta agaagggcag cgcggctccg ccgcgtcgca
    16801 gcggatataa caggattact ccagcaccaa cgtctgcggg tgtggtgttc gccaacgctt
    16861 tggtcacgaa ggcctccact gcgtcatccg gtagaaacaa atccgaccac ggatgcgggg
    16921 tggtccaaac ccccgctgag gcccaaccgg caacgaactg atccacgcga gacgtccaag
    16981 ccaagtaagg caggtcggag atttcggtgg cgagtggcgc aaggtcagcc gagagcgggt
    17041 cgagaggcga gggtggcggt gtcgagtcgt accactgggc acattgtaaa atgtactgcc
    17101 agccaccctc cggccctgga gagaccaatc cttgcagaaa atccaaggtc ttcgttccgt
    17161 ccagcgcacg atgcgctcgc aggtagcgag acaaatccgt ataaaccagc tgatacacgc
    17221 gggccatggc gtgggccgcg cggagccgca gcttggcctg gagaatgagc gcgaattggc
    17281 ccaagccgcc caaagtcgac tcgaatagca cacggtggtg gcgcgaagaa cacgtgacca
    17341 gttcgccctg accggtgacg accgtgagct cctccacttg atcgaccact agcccgtgtc
    17401 ggtgcgagct tccgcccacc cctcccacac tcagcactcc gccaatcgag agatggagat
    17461 agtccgtcaa caccggcgga gtcaatccta ccgcagtagc ccgtgtgacc aactcactcc
    17521 aagtgacgcc ggtctcgacg atcgcgccgt ccgcatcgag ccggtgaatg cggttcaggc
    17581 tgcgcgagtc gatcacgatt ccggagggtg cctgggcttg accgagcacc gaatgcccct
    17641 gcccccgcat cgcgattggg agcccctgct ggttggcgaa acgcaccatc cgcaccacat
    17701 cctcgaagtt tcctgggcgg agcacggccc aggatagacg atgctcgata tgtccgaaat
    17761 cttcagcggc ctcggcgatc gccgtgctct cgaacacgag ctctccacgc aggtcgggag
    17821 ctcggatgca gccagctcgc gcttcggaaa cccaaatacc ggcaacggga tcgaagccgt
    17881 acacagctac agcgctggct gcaccagcga acagctggcg tctcgataaa ttcatgtgcc
    17941 tcctcgtctc aacgaactac tcctgaatcg atgcggccaa gttttgtggc ggcgtcgcgg
    18001 tgttcacggg gcttcgttcg gaacctgcgc gcagcgcacc aggaccggca cgatcccgcc
    18061 atcatagtcc acctcgacct tccggtagtg gcccgagctg gcgtaggcga cgctcggaat
    18121 ataggcgcgc tgcgtggcga ccgttgggca ccggagcgcg aactgccgta aaagctcgtc
    18181 gtccggcagc tcttccgggc tcatggcggg tcgatagaaa gccagacgct ccacgcgctc
    18241 gctatcccaa gcgaaagtaa agtttgcagc caccgagcgc tcgatgtgct ggagccagcc
    18301 ctcggccatg ccgttgaagc tcagctcccg agcgacgctc gcaaccactt cgggtgtggc
    18361 ccagcctgga cgtaaaatag ggtacaaatt gcaggtgcga ttggcgggat cgatcccgac
    18421 aatcgacatg tgctgaagcc catagcgctc gagcacgctt cgtactcgta ccagagcagg
    18481 cggggcgcca ggcagccgtt cgaagcgttc actgcgaaaa ccctggtcga ggaacgccca
    18541 caccttacgg aagccaccgc gcacatcgca atccaaaccc tccgcacgaa tgccgaaggc
    18601 ggacgtgatc gcgtccatga aggcccgggg ctcaccctcg atctcgtacc aatcactcgc
    18661 gatgttccat acttcgccgc gcgcgtcttc gtcaacgaat cgaaatgaga cttcgcgttt
    18721 ggcgaggggc tgactagtcg tcctgatttc gacgggcttg ctcgcgaatc ggtcggcgaa
    18781 gagcgccaaa ccttcggcaa tcttggctcg atccgcgatg acccccgcgt gccttgcgca
    18841 ggtgaccagg tcgttcatca ggcgcgcaga cgcgcggctc tgcgccgcac ttaggatggt
    18901 tgcattcacg ctattcgttc tccggtttgg gggttatgtc ggtggttggg accaggtgga
    18961 ttcgggcgaa cgcgagattg cgagttctgg gagttcgcac ttccaccatc ctgctcatcg
    19021 aaacgctcga gaaccggacg cttcagtcga acgggctctt tgcggtttca gcaccagccg
    19081 gaaattcccg agctaggtgc gtgtaggaca gggtgtcgtg ctcgaatggg agagccgaag
    19141 cttccgccca ttcgtgctcc aaggagcgac atctcgagac gggttctccg gacccgcacc
    19201 tggtgaatac gtgccgttca ccaggctttt gtataggtaa cgtattttct tttggatgcc
    19261 cactttggag gagtggccct atcccagatc agggcctctc gagaatgcct gttcagcgcc
    19321 cgagggatca cgcaaccgaa ctactccgga ggattatgct tgcgacggtg cgggccgatc
    19381 gcgtcgaatc cccagttgag attcgtgaga gggatggcgg ctgctcccac tcgtacctgg
    19441 gatgcggtca gctgtcgcag gacgtgtgcc agtctgtttc ccgacgaccg accctggtcg
    19501 agtcggaaag caatacgaag tcgaaacata ccggtcggcc aggtcccagc agtgcgtgcg
    19561 ggcaggcatg gccgcggcgc cgctcgagaa aaagaatcgc gcgttgtcga tctagccgat
    19621 cccggagcgt cgcgaaggtg agccagctga gacggctcga aagtgagttg tgacgatgag
    19681 ccctcttgct agcaaccgtt tcgtgcctgg cgcgggccca ccgagcggcc ttctaaccca
    19741 tgctgagggc gcgcttcgag gggaactgag gctgaatttg gcgcttccct tgtccgacgc
    19801 aggcaatttc ttgcgctctc ggcctcatct tgggtgccga cgtgctcgcc atacccgtgc
    19861 tatggctgcg cgcgccgata gccaatctga agccgcgcgc ttcggcctgc tcgacgtcgt
    19921 ccgtcgtcgg cgcgaaattc aggctgagct aagcagagca agccgagcca agaccccgaa
    19981 ccgagagaaa acgtaccagg agtgagccga tgcgattcat cgtcatgcac aaggtcgacg
    20041 aaaagatgga agcgggagcg ccgcccaatc aggagatcat ttccgccatg gggagcctcg
    20101 ttcgcgagtc cctgagcaac ggcgtgttcc aaaatggcgc cgggttgcac cggagtgccc
    20161 agcgcgtacg gctctccttt cgtgacggcc ggtgcaccgt gacccagggg ccatatcaag
    20221 gccagaatga actcgtctgg gctttcgcca tgatccagac tcccgcaatg cagggggcga
    20281 tcgagcaggc gtcgcggtac gcagacctgc tgggagactc ggaaatcgag atcggtccgg
    20341 tcgtggaggc ctgggatctc gggatcgttc ccaagccggc gcatatcgag aaaagccgct
    20401 tcctgctgct catcaagggc aacgccgcca ccgagggcaa ccgcctcgac aaggccatgc
    20461 ttcagcgcct gggggagttg gagcagcagt tgacgacgga aggtgtgctg cttgccgccg
    20521 agaaattggc gccc
//
LOCUS       DASTKU010000906         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_178038, whole genome shotgun sequence.
ACCESSION   DASTKU010000906 DASTKU010000000
VERSION     DASTKU010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178038"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="VFQ61_31785"
     CDS             <1..1519
                     /locus_tag="VFQ61_31785"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HET9959130.1"
                     /translation="GVTGLIRAARKVEEGSDRTAAVRFELWQRSRLRQAEFKGQVALK
                     RARIDLGLALGFLLLGCGSAGVGIGPEGGGALGAGGSEPTGGGSTSSGGRAQGGAFNA
                     SGGKLGNAGSGAGLGGFRSSSGGAPNAGSTSLPSGGAQQPSAHGYLHVEANGLFDETG
                     HPARFTGVNWFGFETSNQSPHGLWARDYRSMLKQIKALGFNTVRLPWSDAILRADAKA
                     ASVNTYGEDPYDHTNPMNGDLEGKKPLEMLDAVVIAAGELGLKLILDNHSRDPDGYMN
                     ELVWYSASCSEEQWISDWVAIAQRYKNNPTVAAMDLDNEPHGTATWGGDAQTNWKAAA
                     ERCGNSILEVNPDVLIIVEGVEKVGSDGYWWGGNLSGVKTAPIQLSNPKKLVYSAHEY
                     GPEVHDQPWFQTSDFPGNLPTIWGPHFDFIMQQKLGHVLLGEFGLKARDTYEGRSGQW
                     FDTVLTKLGGTYSWTFWCWNPNSGDTEGILDYDWLTPKQWKLDALRPYMAPPIGG"
     gene            1766..>2592
                     /locus_tag="VFQ61_31790"
     CDS             1766..>2592
                     /locus_tag="VFQ61_31790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9959131.1"
                     /translation="MRILAVVAGLSVANLYYAQPLAAMMAETYHVKSSAIGVTLMLSQ
                     VGYALGMLLLVPLGDGRERRGLMVTTAVAASGSLLLVALAPSYAALAAASLLLGFASS
                     LPQMAVPFAVGLVPAEERGRAIGSVMGGLLTGILLSRTASGMLASGVGWRTTFVCAAV
                     AMALTALVLRTALPTQLPAEPLAYRRILASLVEVVRSEPLLRRHALVGALGFGSFSVF
                     WSTLAFHLATLGYGSKVAGLFGAVGVVGIVAAPIAGRLSTRVQPALLNVFGLLAVVLG
                     "
BASE COUNT          433 a          791 c          893 g          475 t
ORIGIN      
        1 tggagtgacc gggctcatac gcgcggctcg aaaagtggag gaaggttcgg atcgaacggc
       61 cgccgtccgc ttcgagcttt ggcagcgatc gcggctccgt caggccgagt tcaaaggaca
      121 ggttgctttg aaacgcgcac gaatcgacct tggcttagcg ctaggctttc tgttgctcgg
      181 ctgcggcagc gccggcgtcg gcatcgggcc ggaggggggc ggagcgctcg gcgccggcgg
      241 atcagagcct acagggggag gttcgacctc gtcgggggga cgcgctcagg gtggggcctt
      301 caacgcctcg ggaggcaagc tggggaatgc ggggagcgga gcggggctcg gtggttttcg
      361 gagctctagc ggaggggccc cgaacgcggg ctccacgtcg cttccgagcg gcggcgcgca
      421 acagccgagc gcccacggtt acttgcacgt agaagcgaac ggactcttcg acgagaccgg
      481 acatccggcg cgcttcacgg gcgtcaactg gttcggcttc gaaacatcga atcagtctcc
      541 gcacgggctg tgggctcgcg actaccgcag catgctgaag cagatcaagg cgctcggctt
      601 caatacggtg cgcctgccct ggtccgacgc catcttgcgt gcagacgcga aggctgcatc
      661 ggtcaacacc tacggtgagg acccgtacga tcacacgaac ccgatgaacg gcgatctgga
      721 ggggaagaag ccgctcgaaa tgctggacgc cgtggtcatc gcagcgggag agctcgggct
      781 caagctgatt ttggacaatc actcccgcga cccggacggc tacatgaacg agctcgtctg
      841 gtactccgcg agctgtagcg aagagcagtg gatctcggat tgggtagcga tcgcccagcg
      901 ctacaagaac aaccccaccg tcgcggcgat ggatctcgac aacgaacctc acggcaccgc
      961 cacttggggc ggcgacgcac agaccaactg gaaagcggcc gcggagcgtt gcggcaactc
     1021 gatcctcgag gtgaaccccg acgtgctcat catcgttgaa ggcgtcgaga aggtcggctc
     1081 cgacggttac tggtggggcg gcaatttgag cggggtgaag acggcgccca tccaactctc
     1141 gaaccccaag aagctcgtgt actctgcgca cgagtatggg cccgaggtgc acgaccagcc
     1201 ctggttccaa acgagtgatt ttccgggcaa cctgccaacg atttgggggc ctcacttcga
     1261 cttcatcatg cagcagaagc tcggccacgt tcttttggga gaattcgggc tgaaggcgcg
     1321 cgacacctac gaaggtcgct cgggacaatg gttcgacacc gttttgacca agctcggcgg
     1381 gacgtactcg tggaccttct ggtgctggaa cccgaactcg ggtgatacgg aagggatctt
     1441 ggactacgac tggctgacgc ccaagcagtg gaagctcgac gccctccgcc cctacatggc
     1501 gccgccgatt gggggctgag cgcggaaccc gccggtaatt gcgcatctcg agcgcaccgg
     1561 gcccatgtgg cgtgtctgcg tcggtagcgg ttcgtctccc tgcgactggc tgatacggtg
     1621 cgccgttcga gctggcgacc ggctggacga agagctcgcg attggcgtcc ccgatcaaac
     1681 gcgccatgag ttccggatga gccccggagc gaacggcccc attgcgcgag cctcagttac
     1741 gacgagccca tccgctctcg gagccttgcg aatcctggcc gtcgtcgcgg ggctcagcgt
     1801 ggcgaacctg tattacgccc agccgctcgc cgctatgatg gcagagactt accacgtgaa
     1861 gtcgagcgcg atcggcgtga cgctgatgct ctcgcaggtg ggttatgcgc tcggcatgct
     1921 gctgctggtt ccgctcggcg atggacgaga gcggcgcggc ttgatggtta cgactgccgt
     1981 ggccgctagc gggagcctgc tcctggttgc gctcgcaccg agctacgcag cgctcgccgc
     2041 tgccagcctg ctcctagggt tcgcgtcgtc gttgcctcaa atggcggtcc cgttcgcggt
     2101 agggctcgtt ccggcagaag agcgcgggcg cgcgattggc agcgtgatgg gcgggctcct
     2161 gaccggaatt cttctatctc ggacggcgag cggcatgctc gcgagcggcg tcggttggcg
     2221 tacgaccttc gtctgcgccg ccgtcgcaat ggcgttgacc gcgctcgtgc tgcgcactgc
     2281 gttgccgacg cagctacccg ccgagccgct cgcgtatcgg cgcatcctgg cttctctcgt
     2341 agaagtcgtg cggagtgagc ccttgctgcg tcggcacgcg ctcgttgggg cgctcggttt
     2401 cgggagcttc agcgtctttt ggtcgacgct cgcgttccac ctggcgacgc tcgggtacgg
     2461 cagcaaagtt gcaggattgt tcggcgccgt cggcgtggtg ggcatagtgg ccgcgccgat
     2521 cgcgggaagg ctctcgactc gggtgcagcc cgcgttactg aatgttttcg gtctacttgc
     2581 ggtcgtgctc gg
//
LOCUS       DASTKU010000907         6466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1071491, whole genome shotgun sequence.
ACCESSION   DASTKU010000907 DASTKU010000000
VERSION     DASTKU010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071491"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..2853)
                     /gene="acnA"
                     /locus_tag="VFQ61_31795"
     CDS             complement(136..2853)
                     /gene="acnA"
                     /locus_tag="VFQ61_31795"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HET9959132.1"
                     /translation="MADRSRDSYKTRSTLDVAGKSFTFFSLPKLGEALGLDVSKLPFS
                     LRILLENLLRCEDGKAVEKKDVEALAKWDPKATPDTEIAFHPARVVLQDFTGVPAVVD
                     LAAMRDAMQAMGGDPNKINPLFPSELVIDHSVQVDAYGSPEAIQINTKNEYERNQERY
                     VLLRWAQKAFDNFKVVPPSTGIVHQVNLEYLARVVFDQDGVAYPDTLVGTDSHTTMVN
                     GAGILGWGVGGIEAEAAMLGQPINLLVPQVIGFRLYGQLPAGATATDLVLTITRMLRD
                     KGVVGKFVEFYGDGLNHLPLADRATIGNMSPEFGSTCAIFPIDEETLRYLRLTGRSAE
                     RVALVEAYAKAQGLFRVAGQTDPVFTDTLSLDLGTVVPTLAGPRRPHDKVTLRDVPTS
                     FNKTLPELAAAARKTKSAGPVSPEALKTHVPVQYEGHDFELKHGSVVIAAITSCTNTS
                     NPYVLIGAGLLAQNATRRGLTSKPWVKTSLAPGSKVVTQYLTSAGLLPALEALRFHVV
                     GYGCTTCIGNSGPLPDPVSKGIHDGELVAVSVLSGNRTFEGRISPDVRANYLASPPLV
                     VAYALAGRIDIDFDSEPIGVGSDGKPVFLREIWPSPDDVSKAVQAHVTAEMYSREYAD
                     VFVGDERWRAVPVPEGNRFAWDTASTYVRKPTFFENMSLEPQKPSDIGGARVLLMLGD
                     SVTTDHISPAGNIAKTSAAAKYLMEHGVEPKDFNQYGARRGNHEVMMRGTFANVRLKN
                     MLVPGVEGSVTRYLPTGAQMSVYEASMLYQGAKVPLLVLAGKEYGSGSSRDWAAKGTF
                     MLGIRAVIAESYERIHRSNLIGMGVLPLEFVAGENRESLGLTGEETYSIEGISDSLAP
                     RKIVTVRVRAADGNERTFKALTRIDTAVELDYYRHGGILQYVLRQLMKS"
     gene            complement(2985..4154)
                     /locus_tag="VFQ61_31800"
     CDS             complement(2985..4154)
                     /locus_tag="VFQ61_31800"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9959133.1"
                     /translation="MSVPQVLVVGGGFSGVAAAVAARRAGAEVTLIHAQPGASALYSG
                     IVDGQLLEPPISALAEHLGCSVSQHQPVIATFEGSVRAARAHDCELLDLSPLAGRKVG
                     VLDLARDDWDAQALVACYASSAWARATATQFLLLKTPGLSGITPASVLQHASERQIPA
                     YDFALLLEQPARQQRLIEEIGRASLQPDAFLGGPWLGLSCGVARRLSTQLGSRAIGES
                     SGPPGGVAGARFENRRSSLLRQLGIREHRSRVDRVAPAGSRLRVSAKSEEVEVDAVVL
                     ALGGVAVGGVVLEPSSRGFRLSVEAPGGIELDGEELDAASAAFGVSFSRLGLSALQRV
                     GVRTSQNQRIAAELALFACGDVAAGRSRSVSEALSSGYRAGLHAAAAAQRPPAAS"
     gene            complement(4151..5866)
                     /locus_tag="VFQ61_31805"
     CDS             complement(4151..5866)
                     /locus_tag="VFQ61_31805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET9959134.1"
                     /translation="MPDSPAVRSAIPSADSVDVDVIVIGGGVNGTGVLRDCALRGLRV
                     ALFERNDLAFGASGNSSGMIHGGPRYLSADARVTYTSCLDSGHIQRVAPHLLFRIPFI
                     MPVYAESSLAARTTLAAYDAFFARYDRFQPLKRGKPHVRLSAEDVHELEPGLLPSVRG
                     AVSFDEWGIDGSRLCVANALDAVERGRAEVRVHTTVTEVLRREDGRAYGVRYHDRISG
                     ERGRRTARLVVNATGAWAPLTAGLGRLDHTTARVRPGKGIHVFLDRRLTNYAVLTRAV
                     DGRQVFLLPWQNVTVLGTTDDDYYGDLDEVVATEDEVRYLFQAVESVFPTIRQARAIG
                     TWGGVRPTLFGYGVNEDELSREHEIVDHTTHGAPGLFSMIGGKLASYRLFAEEMSDRI
                     ASTLGVREKCATHTLFLPGGEESIDPMRLVATGLVDGVTAARLEYRHGARCLQIVERM
                     QRDSRQAAVVCPCEPVTQAEIVHVVEHELAVSVEDVSRRTRLGLGACGGMRCALSCGR
                     IVARLTGRSPEQGRASAVAFLAGAQRRRVAVQGPEQVRQEALLLAALQGELGETPHNE
                     GGRGS"
     gene            6106..>6466
                     /locus_tag="VFQ61_31810"
     CDS             6106..>6466
                     /locus_tag="VFQ61_31810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959135.1"
                     /translation="MQTPAPPRLTRRLCLLSIVPLWCLLFTRPSFGRGDLPTLGRPQG
                     RPRVKLDRLTFSPEAEQARAYEPQLRKVLQREARRVDWGAGRGSAIQFRFSVEALRFE
                     HRGALLKVHCSALGELPG"
BASE COUNT         1181 a         2211 c         1927 g         1147 t
ORIGIN      
        1 ccacccccat cggctcgcct ggcgccctcg gcacctgcgg atcctgttgc tcttgctgct
       61 cctgcatccg cagtctctta gcatcggctc gcttcgccca ccgccacgaa cgaagcgagc
      121 cgccgatgcc tatgttcagc tcttcatcaa ctgacgcaac acgtactgca ggatgccgcc
      181 gtgccgatag taatcgagct ctaccgcggt atcgatgcgc gtcaacgcct tgaacgtccg
      241 ctcatttccg tccgccgccc gcacccgcac cgtgacgatc ttgcgcggtg ccagcgagtc
      301 agagatcccc tcgatgctgt acgtctcctc acccgtgagc cccagggatt cccggttctc
      361 accggcgacg aactcgagcg gcaaaacccc catgccgatc agattcgagc ggtgaatacg
      421 ctcgtaagac tcggcgataa ccgcgcgaat ccccaacatg aacgtaccct tcgcggccca
      481 gtcacgcgaa gaccccgacc cgtactcttt cccggcgagt accagcaacg gcaccttcgc
      541 gccttggtac agcatcgatg cttcgtacac gctcatctgc gcgcccgtcg gcaggtagcg
      601 ggtgacgctg ccctccaccc ctggcaccag catgttcttg agacgcacgt tcgcgaacgt
      661 gccgcgcatc atcacctcgt gatttccgcg tcgcgcgcca tactgattga agtccttcgg
      721 ctctacgccg tgctccatca ggtacttcgc ggccgcagac gtcttggcga tgttacccgc
      781 gggtgagatg tgatccgtcg tcaccgagtc tcccaacatc agcagcactc gggccccgcc
      841 gatgtcactc ggcttctgcg gctcgaggct catgttctcg aagaacgtcg gcttacgcac
      901 gtaagtactt gcggtatccc acgcaaatcg gttgccctcc gggactggaa ctgcacgcca
      961 tcgctcgtca ccgacgaaca cgtccgcgta ctcacgggaa tacatctccg cggtcacgtg
     1021 cgcttgaacc gctttgctca cgtcgtccgg cgacggccag atctcacgca ggaataccgg
     1081 cttaccgtcg gaaccaacgc caatcggctc gctgtcgaaa tcgatgtcga tgcgccccgc
     1141 gagcgcgtac gccaccacta gcggcgggct cgccaaatag ttcgcgcgca cgtccggact
     1201 gatgcgccct tcgaaggtgc ggttaccgct gagcacgctc acggccacca actcaccatc
     1261 gtgaataccc ttgctcaccg gatccggcag gggaccgctg ttgccgatgc aggtcgtgca
     1321 cccgtaaccg acgacgtgga aacgcagcgc ctcgagcgcg ggcaatagcc ccgccgacgt
     1381 caggtattgg gtgaccacct tggagccggg agccagactc gtcttgaccc aaggcttgct
     1441 ggtaagccca cgccgagtcg cgttctgggc gagcaaaccg gcaccgatca acacgtatgg
     1501 attggaggtg ttggtgcaag aagtaatcgc ggcgatcacc acgctgccat gcttcagctc
     1561 gaaatcatgg ccttcgtact gcaccggcac gtgggtcttc agcgcctccg gcgaaaccgg
     1621 gcccgcagac ttcgtcttgc gcgcagcggc ggcgagctcc ggcagagttt tgttgaacga
     1681 ggtcggcacg tcgcgcagcg tgaccttgtc gtgcgggcgc cgcggtccgg ccagcgttgg
     1741 aactaccgtg cccaagtcga ggctcagagt gtccgtgaac acgggatcgg tctgcccggc
     1801 gacgcggaac agcccttgtg ccttggcgta agcttcgacc aacgcgactc gctctgcgct
     1861 gcgcccggtg agacgcagat aacgcaaggt ctcctcgtca atcgggaaga tggcgcacgt
     1921 cgagccgaac tcggggctca tgttgccgat cgtggctcga tccgcgagcg gtagatgatt
     1981 cagaccgtcg ccgtagaact cgacgaactt accgaccacg cctttgtcac gcagcatgcg
     2041 cgtgatggtg agcacgagat cggtcgcggt cgcgcccgcg ggtagctgcc cgtagaggcg
     2101 gaaaccgatc acttggggca ccagcaaatt gatgggctgc cccaacatcg cggcttcggc
     2161 ttcgatgcca cctacgcccc aacccagaat gccggcgccg ttcaccatcg tggtgtggct
     2221 gtcggttccg acgagcgtgt ccgggtaggc cacaccatcc tggtcgaaca ctacgcgagc
     2281 caagtattcg agattcactt ggtgcacgat gccggtcgac ggcggcacca ccttgaagtt
     2341 gtcgaacgct ttctgcgccc agcgcagcag gacgtagcgc tcttgattgc gctcgtactc
     2401 gttcttggtg ttgatttgga tggcttcggg cgatccgtaa gcatccacct gcacggagtg
     2461 atcgatgacg agctccgacg ggaagagcgg attgatcttg ttcggatctc cgcccatcgc
     2521 ttgcatggcg tcccgcattg cggccagatc gacgaccgcg ggcacacccg tgaaatcttg
     2581 gagcacgacg cgtgccggat gaaacgcgat ctccgtgtcg ggggtcgcct tgggatccca
     2641 cttggcgagg gcttctacgt ccttcttctc gacggccttt ccgtcttcgc agcggagcag
     2701 gttttcgagc aagatcctga gtgagaacgg cagcttcgaa acgtccaaac cgagcgcttc
     2761 gccgagcttc ggaaggctga agaacgtgaa agatttgccg gcgacgtcga gcgtcgagcg
     2821 ggtcttgtaa gagtcgcgag agcgatcggc catgactaat aactcctcgt gcgccgagtt
     2881 actccagagc cgcctcgcac gcaattttga cggctagagg ctaatgcttt tctcggacga
     2941 ggccgaaact ttacagaaac ctgcgccagc tgggtgtggc tatttcagga agctgccgga
     3001 ggccgctgcg cggcagcggc agcgtgaagt cccgcgcgat atcccgaact caatgcctcc
     3061 gagacgctcc gagaacggcc ggccgcaaca tctccacaag cgaacagcgc taattccgca
     3121 gcgatgcgtt ggttctgcga ggttcgaact ccgacccgtt gcagagccga caatcccaat
     3181 cgagaaaagc tgactccgaa ggcggccgag gctgcgtcga gttcctcgcc atcgagctcg
     3241 attccgccag gtgcctccac ggaaagtcga aaaccgcggg agctgggctc caataccacg
     3301 cctcccacgg ctaccccgcc caacgcgagc accacggcgt ccacctcgac ctcttcgctc
     3361 tttgcgctga cacgaagccg ggagcccgcg ggcgcgacgc gatccacccg acttcggtgc
     3421 tctcgaatcc cgagctgccg gagcagcgag ctacgcctgt tctcgaagcg cgctcccgcc
     3481 acgcctcccg gcgggcccga gctctctcca atcgctcgcg accccaactg ggtcgacaag
     3541 cggcgagcca cgccgcaact caagccgagc caaggtccgc ccaggaaggc gtccggctgc
     3601 aggctcgctc gcccgatctc ctcgatcaag cgctgttggc gcgctggctg ttcgaggagc
     3661 agggcaaagt cgtaagcggg gatctgtcgc tcgctagcgt gctgcagcac gctagccggg
     3721 gtgatgccgg acagcccggg ggtcttcagc aaaagaaact gagttgcagt agctcgcgcc
     3781 cacgcgctgc ttgcgtaaca tgcgacgagc gcctgagcgt cccagtcgtc gcgtgctagg
     3841 tcgagcactc ccactttgcg cccggcgagc gggctcaaat ccagaagttc acagtcgtgc
     3901 gcgcgcgccg ccctcacgct gccctcgaac gtcgcgatga ctggctgatg ctggctcacg
     3961 gagcagccga ggtgctctgc cagcgccgag attggaggtt ccaaaagctg accatccacg
     4021 atcccagaat agagcgcact ggcgcccggc tgagcgtgaa tcagcgtcac ttccgcgccg
     4081 gctcgacggg cagcgacggc cgcggcaacc cccgagaagc cgccgcctac caccagaact
     4141 tgcgggacgc tcatgagccg cgtccacctt cgttgtgcgg cgtttccccg agctcgccct
     4201 gcaaagcagc aagcaacaaa gcctcctgtc gaacctgttc gggcccctgc acggccacgc
     4261 gccgacgttg tgcgccggct agaaacgcaa ccgctgacgc gcggccctgt tcgggagatc
     4321 gccccgtcag ccgcgcgacg atacgtccgc acgaaagcgc gcaacgcatg ccgccgcacg
     4381 caccgagccc gagacgcgtg cggcgagaga cgtcctccac gctcacggcc agttcgtgtt
     4441 ccacgacatg aacgatctct gcttgcgtca ccggttcaca cggacagacc acggcggcct
     4501 gtcgcgagtc gcgctgcatc cgctccacga tctgcaggca gcgcgccccg tgtcgatact
     4561 ccaagcgcgc cgccgtgact ccatcgacca aacccgtcgc caccagccgc attggatcga
     4621 tggattcctc gccacccggc agaaacagag tgtgggtggc acatttctcc cgaacgccga
     4681 gcgtgctcgc gatccgatcg ctcatctcct ctgcgaacag gcggtaagaa gccaacttgc
     4741 cgcctatcat cgagaacagt ccgggggccc cgtgtgtggt gtgatcgacg atttcgtgct
     4801 cgcgtgagag ctcgtcctcg ttgactccgt atccgaacaa cgtcggtctg accccacccc
     4861 aggtgccaat cgcgcgcgcc tggcgaatgg tagggaagac gctctccacg gcttggaaca
     4921 ggtagcgaac ttcgtcttcg gtggcgacca cttcgtccaa gtcaccgtaa tagtcgtcat
     4981 ccgtcgtccc gagcacggtg acgttttgcc aagggagcaa gaaaacctgc ctcccatcca
     5041 ccgcgcgcgt gagcactgca tagttggtga ggcgccgatc gagaaacacg tgaatgcctt
     5101 ttccggggcg aacgcgcgcg gtcgtgtgat ctagccggcc cagccccgcc gtcagcggtg
     5161 cccacgcgcc ggtcgcgttc acgacgaggc gcgccgtgcg gcgcccgcgc tcgccgctga
     5221 ttcgatcgtg gtaacgcacg ccatacgctc ggccgtcctc tcgccggagc acctcggtca
     5281 ccgtcgtgtg aacacgcact tcggcgcggc cacgctccac cgcgtcgagc gcgttcgcta
     5341 cgcataaacg agacccgtcg atgccccact cgtcgaagct caccgcgccg cgcacgctcg
     5401 gcagcaaccc tggttcgagc tcgtggacgt cctccgcact caaccggacg tgaggtttgc
     5461 cgcgtttcag cggctgaaag cggtcataac gagcaaagaa ggcgtcgtac gccgcgagcg
     5521 tagtccgcgc cgccaggctg ctctcggcgt acacgggcat gatgaacgga atgcgaaaca
     5581 gcaagtgcgg agcgacgcgt tggatgtgcc ccgagtccaa gcaggaggta taggtgacgc
     5641 gtgcatccgc ggagaggtag cggggccctc cgtgaatcat gccgctcgag ttaccgctcg
     5701 cgccaaacgc caaatcattg cgttcgaaca gcgcgacacg cagcccgcgc aaggcgcagt
     5761 cgcgcagcac ccccgtgccg ttcacaccgc ccccaatcac gatcacgtcg acgtccaccg
     5821 agtctgccga gggaatggcg gaacgcacag cgggcgaatc gggcattcac gttattaaag
     5881 catgcttgcc gcgagcctac ctggtggacg cacgcgcgtg cgtccgtagc ctctgccacg
     5941 ttgtcgcgag tttgttggcc cgagctcctg ccacgtgtaa cggctgcgtg taacggcccc
     6001 gctcacattc accctccccg cccggccggg ctcgaagctc tgcccctgca ggctcgggct
     6061 atgtagctcg tcattcatcc tttcgaacga ttctcctttc gtacgatgca aactccggcg
     6121 ccgccccgcc tgacccgccg cctgtgcctg ctctcgatag tgccgctttg gtgcttgctt
     6181 ttcacgcgcc cgagctttgg ccgcggtgat cttccgacgc taggcaggcc gcagggtcgg
     6241 ccgcgcgtga agctggatcg gctcacgttc tcaccggagg cggaacaggc gcgcgcctac
     6301 gagcctcagc tgcgcaaagt gctgcaacgc gaagcgcgtc gcgtggactg gggagcgggc
     6361 agaggtagcg cgatccaatt tagattcagc gtcgaggcac tgcgcttcga gcaccgagga
     6421 gccctgctca aggtgcattg cagcgcactc ggtgaactgc ccggcc
//
LOCUS       DASTKU010000908         8594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_178655, whole genome shotgun sequence.
ACCESSION   DASTKU010000908 DASTKU010000000
VERSION     DASTKU010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8594
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178655"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(521..1786)
                     /locus_tag="VFQ61_31815"
     CDS             complement(521..1786)
                     /locus_tag="VFQ61_31815"
                     /inference="COORDINATES: protein motif:HMM:NF015500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HET9959136.1"
                     /translation="MTNRSVSIPVLLLLLPACGASTDGGPLLPLGSAGAGLGGTQGGQ
                     TSASGGTSGTSVPNVSGGLGGAQPTGGTYGGGSGGVRSTSGGSSMTSASGSPSTGGAS
                     ATSTGGVPSTGGTVSTGGKATTGGTASTGGRTSMGGRASTGGRSSTGGAGGTNTAGGT
                     SSAGAGSGGMAQGGQSGGAGGAGSFRLPPANAPFDYQIGGAYAPPQGVQVVSRDRESS
                     PAAGLYNICYVNGFQSQPGEDKLWLEDHPDLVLRDSSGEPVIDENWDEMLLDTSTEAK
                     RTELATIVGGWIRGCASSGFNAVEIDNLDSYSRSGGLLKEANNIAFMKLLSAAAHQSG
                     LAIAQKNSSELVGQAKQMGTDFAVVEECNRWDECGDYQSGYGNLVFIIEYRRQDFTKG
                     CTGHPELSIVLRDLNVSAPGNSSYVYDGC"
     gene            complement(1973..2737)
                     /locus_tag="VFQ61_31820"
     CDS             complement(1973..2737)
                     /locus_tag="VFQ61_31820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9959137.1"
                     /translation="MHTAGRRGATRVVSFELMRTPPPTPDDPTVYPVEEPVGEEILQC
                     WMLEVLRPLIERWLAERGTPCFVGSDQFIYFEKGQVSQRIAPDIYVLPGVPQDLDIPS
                     WKIWESGIHPSFALEICSQDWRKDYEMAPRLHHQIGTEELIVVDPDYLKRREGYRLQR
                     FERTSRGLELQERTQADRVRSRVLGCFVRLVGTERPRQRVRLALDPDGDILFPTHAEA
                     ERQRAEEQRKSADAERQRAEAALARVAELEALLSRH"
     gene            3287..3424
                     /locus_tag="VFQ61_31825"
     CDS             3287..3424
                     /locus_tag="VFQ61_31825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959138.1"
                     /translation="MVVANDCDRGGVLRGGEAVYGGATLINEAAAGRVLFTPGDCNER
                     W"
     gene            3429..4979
                     /locus_tag="VFQ61_31830"
     CDS             3429..4979
                     /locus_tag="VFQ61_31830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959139.1"
                     /translation="MKRRTRTLQGTFVSALLGVLVLGAGCSGDVAPSESAEVETATQK
                     LDIIETVVKEKYLDPVTAKNLKAVFELGKDVADAFTGFGKAVDVATKLAEKLGFISGK
                     PDFEAVMLEQLKKIQEQIQGVLTFEENAFWLESEQTLSGIYNDAQAVSESTRDWVVQH
                     PGQVLPLDSPLGALATQVSKTATANFAEDVWYRFPDTKGRHFEFRFALPRFTHVIAMR
                     LGVLAATTPDFVQTGSASVELLRYHDALGRILAEMERTYISCYSPQAVPIPQGSDPSS
                     LITRRCANKLTGTDVVVSTVGTPGTEAPIRGQLLDELGAAPIRELRERLFQLATNAFS
                     GSSACSGEGGTCLFAGVQTVRYGALASYYFRNAQGSIPCNNGFWGDPVGGTAKSCAAG
                     PLIWSPCGIEGENCFFPGPKMVRYGANGAFKQKFTFAGVGCTNPDFGGDPIFGVQKSC
                     SYTDPIWTKCADEGGQCALSGKHYVRYGHGSNWAYRERTGNVPCNNGLFGDPAPNVEK
                     VCEVAAAF"
     gene            complement(5142..5333)
                     /locus_tag="VFQ61_31835"
     CDS             complement(5142..5333)
                     /locus_tag="VFQ61_31835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959140.1"
                     /translation="MRLVGTERPRQRVRLALDPDGDILFPTDAEQAEEQRQRADAERQ
                     RAEAALARVAELEALLSRH"
     gene            complement(5452..5574)
                     /locus_tag="VFQ61_31840"
     CDS             complement(5452..5574)
                     /locus_tag="VFQ61_31840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959141.1"
                     /translation="MHTAGRRGATRVVSFELMRTPPPTPDDPTVYPVEEPVGES"
     gene            complement(5769..6119)
                     /locus_tag="VFQ61_31845"
     CDS             complement(5769..6119)
                     /locus_tag="VFQ61_31845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959142.1"
                     /translation="MSFPLQHVEIVGLVFWVMSLLFLARARQALIITCHLLCAALSFA
                     FPVLAALRADHLSLTTLVQIWVLGLLNGLMGSAWCARLDPPIPKAPMLGITAAGLGAA
                     LLIVFMIATPTRLF"
     gene            complement(6314..8191)
                     /locus_tag="VFQ61_31850"
     CDS             complement(6314..8191)
                     /locus_tag="VFQ61_31850"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HET9959143.1"
                     /translation="MAITSLLAMCAFSPGARADYPVASHRYLADPGSLVFNGRIYLYN
                     SNDDDNPVSGGYQMKSIVCVSTSDLKNWTDHGVVFSVPAGASWASNSWAPQPIERNGT
                     IFLYFGNSGSGIGVASSKDPVSGFKDAKGSYLINGSTPGASGTDSWLFDPGALIDEDG
                     QAYLAFGGNGENNARIIKLGSDLASTSGSAAQLSPKGFFEASFLFKRDKTYYLAYSTN
                     PANGLRIDYLKSSSPMSGFTYGGVIADQPPQNGDNNHASEFVFKDKWYHAYHNRVVAK
                     QAGISATYRRNLGIEVLDFNADGSIKQVTYTTDGVPQVGTVDPYVRFEAETMNAQKGI
                     ETETCSEGGMNVTQLSNGDWIKVRGVNFGASGAKAFRARVASTAAGNTLEIRAGSETG
                     KLLGTCNVPMTGGAQTWSSVDCDVTGATQVMDLVFKATGSGSQSFNFNYWQFIPLDGG
                     MNGSGGASGSGGASTNGGASGGAQSGGSSQSGGAVMGGAGGGPAPGSGGTLSGAAGGA
                     NAGGGGTSLAGMGGSSGGQGSGGVNAGATAFGGATASSGGTLASSSGGTLSGAGAPGA
                     AGGVTSGTAAAGPGPAGVSDSAGCACTVGAQSRRSTAPFALLALAAAAVGRRRRRIAE
                     R"
     gene            complement(8384..>8594)
                     /locus_tag="VFQ61_31855"
     CDS             complement(8384..>8594)
                     /locus_tag="VFQ61_31855"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HET9959144.1"
                     /translation="EYLLGTENIAASRAVMIGDRAHDMIGAKRNAVRALGVLWGYGDR
                     AELFDAGADAVVATVSELVLALREC"
BASE COUNT         1557 a         2667 c         2756 g         1614 t
ORIGIN      
        1 acgtcgtcgt atttgtggag gattagaccc ctgacggtcg cgcaggggag atccccagag
       61 ccgcagataa gctgtatttc cgtcatcgaa tcaagccctg agcggcgcag caagaaaggc
      121 aattcagatg ccgttagccg agaagttttt cagcgacacc cagcgccacg cattcgctag
      181 aggtcgggcc gagggtgaga tcaaaggcag agctgaaggt agagccgaag gcagagctga
      241 ggcgcaagca gagttcgtcg tgcacgtgct gacgatccgt gggctgagcc cgtcggactc
      301 tcaacgcgaa cgcatactct cctgtcgaga tagtgagcag ctcacccgtt ggctggatgc
      361 cgcgctgtgc tgcagcagtg tggacgagct actgagttga agagacctca ttcgggcgct
      421 ccgccgcgag gctcgtcaga ggcgggcgag aaccggcctt aatcgtgatc ggagcgcacg
      481 tagggtgttc cgtaactcca agtttttggc gaggcgaggg tcagcacccg tcgtagacgt
      541 agctcgagtt gccgggggcg ctgacgttga ggtcgcggag aacgatactg agttcggggt
      601 ggccggtgca gcctttggtg aagtcttgtc gacgatattc aatgatgaag acgaggttgc
      661 cgtatccgga ctggtagtcg ccgcactcgt cccagcgatt gcactcttcg acgacggcga
      721 agtcggtgcc catttgtttg gcttggccaa cgagctccga cgagttcttc tgggcgatgg
      781 cgaggccgct ttggtgagcc gcggcggaga gcagtttcat gaaggcgatg ttgttggctt
      841 ctttgagtaa gccgcccgag cgcgagtagc tgtcgaggtt atcgatctcg acggcgttga
      901 agccgctcga ggcgcagccg cgaatccaac cgccgacgat ggtggcgagc tcggtgcgtt
      961 tggcttcggt gctggtgtcg agcagcattt cgtcccagtt ttcgtcaatg acgggctcgc
     1021 cgctgctgtc gcgtaggacc aagtcgggat gatcctcgag ccacaacttg tcttctccag
     1081 gctgggattg gaagccgttg acgtagcaaa tgttgtagag cccagcagca gggctactct
     1141 ctcggtcgcg gctgacgact tggacgcctt gtggcggagc gtatgctcct ccgatttggt
     1201 aatcgaaggg tgcgttggcc ggtggcagac ggaaggagcc cgcgccgccc gcgcctccgc
     1261 tctgtcctcc ctgggccatt cccccggatc cggcgccagc actggatgtt ccacctgcgg
     1321 tattggttcc tcccgctccg cccgtcgatg atctgccgcc cgtggacgct ctgccgccca
     1381 tggacgtgcg gccgcctgtg gatgcggtgc cgccggtggt cgctttaccg cccgtcgaga
     1441 cggtgccacc cgttgatggg actccgccgg tcgaagtcgc gctcgctccg cccgttgatg
     1501 gggagccgct ggccgacgtc atgcttgagc cgcccgatgt agatcgaact ccgccggagc
     1561 cgcccccata ggtgccaccc gtgggctggg ctccacccaa acctccgcta acgttgggta
     1621 ccgacgttcc ggacgtaccg cccgatgcgc tcgtctgacc accttgggtt ccacccaggc
     1681 ctgctcctgc gctgcccaaa ggcaacaaag ggccgccgtc cgtgctcgcc ccgcatgcgg
     1741 gaaggagcaa gaggagaacc gggatggaga cgcttcgatt ggtcatgtca cccctgtggc
     1801 ctgcgtcgtg aaaaagctgg tcagcgaatc gtcacgtgcc cttttccgga agcccctgtg
     1861 cactcgggtg gccgctcgcg actcactgtc ccgcggtgag cttggggcgc gctcgacgct
     1921 cacccggctg cccgtaggca agcccctacg tttagcgcgg tgggttggat gctcaatgac
     1981 gagacagcaa cgcctcgagc tcggcgacgc gtgccaacgc tgcttcggcg cgttgcctct
     2041 cggcgtcggc gctcttgcgt tgttcttccg cccgctggcg ttcggcctct gcgtgagtgg
     2101 ggaatagaat gtcgccatct gggtcgagcg ctaggcggac gcgctggcgc ggccgttcgg
     2161 tgccgaccaa gcggacgaaa catccgagta ctcgtgaacg cacgcggtcc gcctgggtgc
     2221 gttcctggag ctcgagccct cgcgaggtgc gttcgaagcg ctgcagacga tagccctctc
     2281 tgcgttttag gtaatctgga tctacgacga tgagctcctc ggttccaatt tgatggtgga
     2341 gccggggagc catctcatag tccttccgcc aatcctggga gcagatctcc aaagcgaagc
     2401 tcggatgaat gccactctcc cagatcttcc aggaagggat atccaaatcc tgaggaacgc
     2461 ccggtagcac gtaaatgtcc ggagcaatgc gctgtgagac ttggcccttc tcgaagtaga
     2521 tgaactgatc agaccccacg aaacacggag tgccgcgttc cgcgagccag cgttcgatga
     2581 gaggtcgcag cacttcgagc atccaacatt gcaggatttc ttcccccacg ggctcctcca
     2641 cgggatacac cgtcgggtca tccggagttg ggggcggagt gcgcatgagc tcgaacgata
     2701 ccacgcgcgt ggcgccccgc cgaccggcag tgtgcatgca gggctattta gcatgaggcg
     2761 acgagaatgc ctgaacaaaa gagcacaaaa tcacgcagga atcagggttt atcccgcggc
     2821 tcgcaccgca ggcactaggg atatcccgag tgccgttcat ggtctccaca acggcccgcg
     2881 ttgcggaaca ctgccgctcg cgagctgagt cgcatgccga gcccagagcc accgcgcgcg
     2941 tgcagtagcc cgcgccggcc tcgtcaagcg ttatagggaa cgttcagctg caggtgccgc
     3001 tctcggcgac gcgagggggt cgcaccggca ggcgaacgga gcgtatccta atcgagccgc
     3061 tgcatgaatt cgggtcgtgc tcttggcact ctcctcgatt gagtccgatt cgagcgcgga
     3121 gaattaccta gtgctggtac acgtggagcg gtacacgtgt accactcggg agtgaacacg
     3181 atggttgtgc gccggtcgag accgggcttg agctcgagtt tcggcgtcga gtttcggggc
     3241 gctgacgcag atcccacgaa gtttcgtgag gtcagctttt cgcggcatgg tcgttgcaaa
     3301 tgactgcgat cgtggcggcg tcttgcgcgg tggggaggcc gtgtacggtg gcgccacgtt
     3361 aatcaacgag gcggccgcgg ggcgcgtctt gtttactcca ggagattgca atgaaagatg
     3421 gtgagtccat gaaacgtcgc acgcgcacgc tgcaggggac cttcgtcagc gcattgttgg
     3481 gtgtcctcgt gctgggcgcg gggtgcagtg gggatgtggc gccgagcgag agtgccgagg
     3541 tcgagacggc gactcaaaaa ctcgatatca tcgagaccgt cgtgaaggag aagtacttgg
     3601 acccggtgac ggccaaaaat ctgaaggccg tcttcgaact cgggaaagac gtcgcggacg
     3661 cgttcaccgg cttcggaaaa gcggtggacg ttgccacgaa attggccgag aagctgggct
     3721 tcatctcagg caagcccgac ttcgaagcag tgatgctgga gcaactcaag aagatccagg
     3781 agcagatcca gggtgttctg accttcgagg aaaacgcatt ctggctggag tccgaacaga
     3841 cgttgtccgg gatctacaac gatgcccaag cggtgtccga gtcgaccaga gattgggtgg
     3901 tgcaacaccc gggccaggta ttaccgctcg actcaccgct cggtgccttg gccactcagg
     3961 tgtcgaaaac cgcgaccgca aacttcgccg aggatgtttg gtatcggttc cccgatacca
     4021 agggcagaca cttcgagttt cgtttcgctc tgccgaggtt cactcatgtc atcgcgatgc
     4081 gcctcggagt gttggcggcg accactccgg acttcgtaca aaccggctcc gcgagcgtgg
     4141 agctgctccg ctaccacgac gcgctcggac gcatcctcgc ggagatggag cgaacctata
     4201 tctcctgcta ttcgccgcag gccgtgccga tcccccaagg ctcggatccg agctccctca
     4261 tcacgcggcg ttgcgcgaac aagctgacgg ggaccgatgt ggtcgtcagc accgtcggga
     4321 cgccgggaac cgaggccccg attcgaggtc agttgctcga tgagctcggg gccgccccga
     4381 ttcgcgagct acgggaaagg ctgttccagc tcgcaacgaa cgccttctcg ggctcgtccg
     4441 cttgttctgg cgaggggggg acttgcttgt tcgctggcgt ccagacggtt cgctacggtg
     4501 cgctggcctc gtactacttc cgaaacgctc aaggcagcat cccgtgcaac aacggcttct
     4561 ggggagatcc cgtgggcggg accgccaagt cgtgcgccgc ggggccactc atctggtccc
     4621 cctgcgggat tgaaggcgaa aactgcttct tccccgggcc aaagatggtg cgctacggcg
     4681 ccaacggcgc attcaagcaa aagttcacat tcgcaggggt tggctgcacc aaccccgact
     4741 tcggagggga tccgatcttc ggcgtgcaga agagctgctc ctatacggat ccaatctgga
     4801 ccaaatgcgc ggatgagggc gggcagtgcg ccctctctgg caaacactac gtgcgttacg
     4861 ggcacggtag caactgggcg taccgcgagc gaacggggaa tgtcccctgc aacaacgggc
     4921 tcttcggcga ccccgcgccg aacgtcgaga aggtatgtga ggtcgcagcc gcgttctgaa
     4981 cgggccgagt ggcgggggtg acggtgcacg cagctgcgcc gtcacctcga ctcgctcgcg
     5041 gggccgttcg aagtccaact ctgcaagccg gcctcgaagc ttccttggtg aggagtgcgc
     5101 tgagttgctg ggcgaacgcg ggcgcgacgg cttggatggg ctcaatgacg agacagcaac
     5161 gcctcgagct cggcgacgcg tgccaacgct gcttcggcgc gttgcctctc ggcgtcggcg
     5221 cgctggcgct gttcctcggc ctgctctgcg tcggtgggga ataggatatc gccgtctggg
     5281 tcgagcgcta ggcggacgcg ctggcgcggc cgttcggtgc cgaccaaacg cacgaagcat
     5341 ccgagtactc gcgaacgcac ccggtccgca tgggtgcgtt cctggagctc gagccctcgc
     5401 gaggtgcgtt cgaagcgctg cagacgatag ccctctctgc gttttaggta atcaggattc
     5461 cccgacgggc tcctccacgg gatacaccgt cgggtcatcc ggagttgggg gcggagtgcg
     5521 catgagctcg aacgatacca cgcgcgtggc gccccgccga ccggcagtgt gcatgcaggg
     5581 ccatctagca cgcgctacga gaatgcctga ataaaagacc acaaatcacg caggaatcag
     5641 ggtttatccc gcggctcgca acacaggcac tagggatatc ccgagtgccg ttcatggtct
     5701 ccacaacttc aggattgttc cgtcctgcgc ttgcgactta cgccgccgct cacccagcat
     5761 ccagcacttc agaacagccg cgtcggggtc gcgatcatga agacaatcag cagtgcggca
     5821 cccagcccgg ccgcggtgat gcccagcatc ggtgccttgg ggatcggcgg atcgagccgc
     5881 gcgcaccacg cgctgcccat caatccattg agcaggccca gtacccaaat ctgcacgagc
     5941 gtagttaagg agaggtggtc ggctcgcagc gccgctagca cggggaatgc aaagctcagc
     6001 gcagcacaaa gcaggtggca cgtaatgatt agtgcctgcc tcgcgcgggc caaaaacagc
     6061 aggctcatca cccaaaacac cagacccact atttcgacgt gctgcagagg gaagctcatt
     6121 cgcgggtatc agctgaacgt acagagggcg tatgcggccg tgcccccgcc ggttcctccg
     6181 ttgaccgggg acgggggctc gtcatcacca acacaccgaa gaagagaaag gacccggtcg
     6241 catagggtga cggcgcatgc cagcttggct gcgcggcgtg accggcggcg ggcgccggaa
     6301 cgtcgtgttc cgttcaacgc tccgcaatgc gccttcgacg tcgacccacg gcggcggcgg
     6361 caagagccag caatgcgaac ggggcggtcg agcggcgcga ttgagcgccg acggtgcagg
     6421 cgcacccggc gctgtcgctc acgcccgcag gaccagggcc ggccgctgcc gtgccggagg
     6481 tgactccgcc tgccgcgccg ggtgctccgg cgccgctgag agttcctccc gaggagctcg
     6541 ccagggttcc accgctgctc gcggttgctc caccgaaggc cgtggctcca gcgttgacgc
     6601 cgcccgaacc ttgtccgcca ctgctgccac ccatgccggc tagactcgtg ccgccgccgc
     6661 ccgcgttcgc ccctcctgct gctccgctca gggtgcctcc gctgccaggg gcaggccctc
     6721 cgcccgctcc acccatgacc gctcctccgc tctgtgatga tcccccgctc tgtgccccac
     6781 cactggcgcc gccattcgtt gacgctccgc cgctgccgga ggcgccaccg ctgccgttca
     6841 tgcccccatc gagagggatg aattgccagt agttgaagtt gaaggattgc gagccgctgc
     6901 cggtggcttt gaagacgagg tccatgacct gagtggcgcc ggtgacgtcg cagtccacgc
     6961 tactccaggt ttgggcacca ccggtcatgg gcacattgca cgttccaagt agcttgccgg
     7021 tctcgctgcc cgctcgaatc tcgagggtat tgccggctgc cgtgctcgcg acacgggcgc
     7081 gaaaagcctt ggccccactc gcaccaaagt tcacgccgcg aactttgatc caatcgccgt
     7141 tgctgagctg ggtgacgttc atcccgcctt cactgcaggt ttcggtttcg atgcccttct
     7201 gcgcgttcat cgtctcggct tcgaagcgta cgtacgggtc caccgtaccg acctgaggaa
     7261 cgccgtctgt cgtgtaggtg acctgcttga tgctcccgtc cgcgttgaaa tccaaaacct
     7321 cgatgccgag gttgcgacgg tacgtggcag atatcccagc ttgcttggcc accactcggt
     7381 tgtggtaggc gtgataccac ttgtctttga atacgaactc agaagcgtga ttgttgtcgc
     7441 cgttctgagg gggctgatcc gcgatcactc cgccgtacgt aaagcccgac atcgggctgc
     7501 tactctttag gtaatcgatg cgcaggccat tcgccgggtt ggtcgagtag gctaggtagt
     7561 aggtcttatc ccgcttgaat aggaatgagg cttcgaagaa gcccttgggc gagagctgcg
     7621 cggcgctacc ggaagtacta gccaagtcgc tgccgagctt gatgatgcgg gcgttgttct
     7681 cgccgttgcc gccgaaggcg agatatgcct gaccgtcctc gtcgatcaga gcgcccgggt
     7741 cgaacaacca actgtccgtg ccggaggccc ctggcgtact cccgttgatg agatagctgc
     7801 ccttcgcgtc cttgaagccg ctgactgggt ctttgctgga tgcgacccca atcccactcc
     7861 cgctattgcc gaaatacagg aaaatggtgc cattgcgttc aatcggctgc ggcgcccagg
     7921 agttgctggc ccagctcgct cccgcaggaa cactgaacac cacaccgtga tcggtccagt
     7981 tcttcaggtc gctagtcgag acacacacga tcgacttcat ctgatacccg ccgctgacgg
     8041 ggttgtcgtc gtcgttcgag ttgtacagat aaatgcgccc gttgaagacg agcgaacccg
     8101 ggtccgccag gtagcggtgg gacgccactg gatagtctgc ccgagcgcca gggctgaacg
     8161 cgcacatggc aagcaacgac gttattgcca tcccgtggca agtacgattc gaccggtagt
     8221 gcatagcgaa acggacccga ccccgaccag cgggggccaa cgcggtgatt gaacggctcc
     8281 atggggcagt ggcgagcgcc ggcctcgtcc gtcagaaaat gagaggtggc ccgcgatggg
     8341 cccgctgccc tcggcccacc gcggctccaa agcgtgacgg gggtcaacac tcgcgaaggg
     8401 cgaggaccaa ttccgagacg gtggcaacca ctgcgtccgc cccggcgtca aacagctccg
     8461 ctcgatcgcc gtagccccaa agcacaccga gggcccgcac ggcgttgcgc ttggcaccaa
     8521 tcatatcgtg ggctcgatcc ccgatcatca ccgcgcggct cgccgcgata ttctctgttc
     8581 cgagcaagta ttcg
//
LOCUS       DASTKU010000909         7595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1741615, whole genome shotgun sequence.
ACCESSION   DASTKU010000909 DASTKU010000000
VERSION     DASTKU010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741615"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFQ61_31860"
     CDS             complement(<1..221)
                     /locus_tag="VFQ61_31860"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HET9959145.1"
                     /translation="MPADFPDLPVPEDNVPTPARIALGRTLFYDERVSRNQEVSCASC
                     HKHANGFADPEQFSLGVDGQRGARNASSLV"
     gene            complement(362..1408)
                     /locus_tag="VFQ61_31865"
     CDS             complement(362..1408)
                     /locus_tag="VFQ61_31865"
                     /inference="COORDINATES: protein motif:HMM:NF042462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnP family protein"
                     /protein_id="HET9959146.1"
                     /translation="MVPPFAVMGLVLRRIACYVGLGVAVWACGENSGNSHAPANPSAG
                     AAGQPSSGSPNAAEIAGTGGNQQAGGNTPATGGGTATGGTAQPPSAGGRGALGAAGAS
                     GGQGASGSATECPETPARPPSAGARLELPVRFTSGGVPLTVGEPAHDSLGEPYRVTLL
                     KIFLSRPELETETGERVPAAFADVAGNPLLYGLHLLDLEDVEPKLRMIVAPGQYTALH
                     LTVGLPEGCNGGDPTRRKFPLNADSDMYWSWGSSYLFVRFEGQSLGEPTHGFSYHLGA
                     LGEGSEPRGVRLEGALLVGESGGPSSIAISLDVGKVIAPAPEDSASVGPHQISAPWML
                     DHIGQDAFSLGPEL"
     gene            1506..2774
                     /locus_tag="VFQ61_31870"
     CDS             1506..2774
                     /locus_tag="VFQ61_31870"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF005121.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HET9959147.1"
                     /translation="MSEIKQKVAILTAGGLAPCLSSAVGGLIARYSELAPELEIIGYL
                     GGYKGLLLGNSVAVTPEVREHAEVLHKHGGSPIKNSRVKLTNVKDCVKRGLVKEGEDP
                     LQVAAKQLTRDGITILHTVGGDDTNTTAADLAAYLGKHGYKLTVVGLPKTIDNDVYPI
                     RQSLGAWTAAEQGAIYFENIVNEHSANPRMLIVHEVMGRNCGWLTAYTAKIYRERLRH
                     PAFLPSFNLARSHKDLDAVYVPEMEFDLEAEAKRLRGVMDEQDCVNIFVSEGACVSQI
                     VKEMEAKGEDIPRDAFGHYKLDAVNVGQWFSKQFAKLLGAEKTLVQKSGYFARSAAAN
                     GDDLRLIQSMVDHAVECGLRGESGVIGHDEDRGGRLRAIEFPRIKGGKPFDIDQPWFN
                     ELLNDIRQPKGAHTNAGHGHDAPSIPPPGA"
     gene            2885..4003
                     /locus_tag="VFQ61_31875"
     CDS             2885..4003
                     /locus_tag="VFQ61_31875"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET9959148.1"
                     /translation="MRISGADFDILLERCTAQLGGLIGNARQHATSRDWLRLRVLSDL
                     GSGIGEACPLPGYSPDDLVEAQRSLEAFPLPSLPPLEQLARAQAPASVLFAALDGADA
                     SLSVSARFALETAFLDLISKSQSLPLHRILGGTATSPIPLSALLPVARASTAELLDAG
                     REWVANGFSNLKVKLGSSASWQDEFAALRELRTTLPASVVLRGDANQGWPIEGATSKL
                     EDCAALGFEFIEEPVARARLAALPRSAVPLALDESLQAAGAEAAFAEWTQRGDVRVLV
                     LKPTTLGGFSRCLRLAARARQLNWDVVVSHCFDGAVAHAAACELALALGCTRACGLGP
                     HAALGPSAESASLIPQLQGAWLVPRPEPGHGVNVDQLD"
     gene            4118..4702
                     /locus_tag="VFQ61_31880"
     CDS             4118..4702
                     /locus_tag="VFQ61_31880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959149.1"
                     /translation="MTLALLYGASSGALHAVTGPDHVLSLGPVALKQPRSPWKIGLLW
                     GVGHALGTLALALPLVWWSTVAPLPWLAALGDRLGGVALMLMAGWSWWSLRRPLSSKD
                     ADTRRPWLIGLVHGITGATSLLLVLPMLVSGSPSLTLTYLLAFALGSTLAMATLTSAL
                     ARLGQRLSPPLVTRLQRSLVGVSFAVGALWLARA"
     gene            4835..5290
                     /locus_tag="VFQ61_31885"
     CDS             4835..5290
                     /locus_tag="VFQ61_31885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959150.1"
                     /translation="MLRAYGSLILSAMIHAQAILRTIALLGLTLSAGPALAAEPTVRA
                     EMECGAASNVVLCVVKFEPSHAAHITWAEASIVAAPPFTSPVLPKATYRESAIKKAHL
                     RLVLLPKGAGQGTIVVKGRGVVCAGDGTSCPHFSKTLSAIVSVAGKSKK"
     gene            5537..6223
                     /locus_tag="VFQ61_31890"
     CDS             5537..6223
                     /locus_tag="VFQ61_31890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959151.1"
                     /translation="MHPLKGRERHGANPPLPWESNGGLPERLWGTALLTSVEPQRAFG
                     ELPEGKLGPAFTFAVIAELYAIGSVTAFLILSALVIDAEFTRVLLSNPLVTGVTFGVT
                     LGLSVIMVLLHALWGVTLEFGLRTAGRHANYVQGIRFGLYACGWDLLTSPAGIAQGLL
                     SRGLWGAWGPIRAAVGVPRSALHAYVTECRKLDISAARRGLRFSLAILVVSLVGFALL
                     TIVAIALWLL"
     gene            6795..>7595
                     /locus_tag="VFQ61_31895"
     CDS             6795..>7595
                     /locus_tag="VFQ61_31895"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET9959152.1"
                     /translation="MSMIISSGHVHGAQTHNHDENAYERIDEVELQRLSLEHAWIVKP
                     IHRASSAILDLPFFHWVRGLKKPIDFKPAAVQLYYHSATFPKVMGLMLGLTSMRENPM
                     MPFYSKHAFGEADHHMMLMRWMLQHGILNSAAEIEDVTVSIETNACVNLAYQLAMEQD
                     RDKWLVAINSGIERCSNDFFKIVAPTMHKIGAGDKYFDIHVEADEHHSIMGLKHLSPL
                     DPSSRRAKQLVSKALEGISLWAGMLHSWIGLQVFPHFDLEGNVIPAQSS"
BASE COUNT         1369 a         2454 c         2313 g         1459 t
ORIGIN      
        1 accaagctag aggcgttgcg ggctcctcgc tgtccgtcca cgcccaaact gaactgttcg
       61 ggatcggcga agccgttcgc gtgtttatgg caactggcac acgacacctc ctgattacga
      121 gacacgcgct cgtcgtaaaa caacgtacgt ccgagtgcga tgcgcgcggg cgtaggcacg
      181 ttgtcctccg gcacgggcaa gtcggggaaa tctgcgggca cccggaggct cactccggtc
      241 tggctcgcgg tcgcgcccct tggcccaccc gacacgctct catcatgcgc agagcacccg
      301 gaactcacta accacgtcag cgatgcagcc ctcagcaacg cgcgagtccg tcgggaagat
      361 ctcaaagctc aggtccgagg ctaaaggcgt cttggccgat atggtctagc atccaaggcg
      421 cagagatttg atgtggcccg acgcttgccg aatcctcggg agcgggcgcg atcaccttgc
      481 ctacatccag ggagattgcg atcgaactcg gccccccaga ttcaccaacc aggagcgcgc
      541 cttccaggcg aacaccgcgc ggctcagagc cttcgcccaa tgcacccaag tggtacgaga
      601 agccgtgagt aggctccccc aacgactgcc cttcgaagcg aacgaatagg tagctgctac
      661 cccaactcca gtacatgtcg ctatcggcgt tcaaggggaa cttgcgacgc gtcggatctc
      721 cgccgttaca cccttcgggc aagccaacgg tcaaatgcag cgcagtgtat tgcccgggag
      781 cgacgatcat tcgcaacttg ggctctacgt cttccaaatc caacaggtgc aaaccgtaga
      841 gtagcggatt gcccgcgacg tcggcgaacg ccgccggcac tcgctctccc gtctccgtct
      901 cgagctcggg ccgtgacaga aaaatcttga gcagcgtaac gcggtacggc tcacccaggg
      961 aatcatgcgc tggctcgccc acggtaagcg gcacgcctcc gctggtaaac cggacgggta
     1021 gttccagccg agcaccggcc gaaggcggcc gcgccggcgt ctccggacac tccgtcgcgc
     1081 ttccgcttgc tccttggcct ccgctcgctc cggcggctcc aagcgctccc cttcctccgg
     1141 cgctgggggg ctgtgccgtt cctccggtcg ccgttcctcc gccagtcgcg ggagtgtttc
     1201 ctccggcttg ctgatttccg ccagtacccg cgatctcagc agcattgggg ctcccgctgg
     1261 acggctgccc cgcggcgcct gctgaaggat tggccggagc gtggctgttt cccgagtttt
     1321 cgccgcacgc ccaaacggcg acgccgaggc ccacgtaaca ggcaatccgc cgcaaaacga
     1381 gacccatcac ggcgaacggt ggcaccacgc ttgcagctcg tcaacgcggt cgcgaacagg
     1441 gcttgcgttt ctcgcggtat cacgtagcaa ttcgcgggca tcaaccaccc gaatggagtt
     1501 tttcagtgag cgagattaag caaaaggtcg ccattctcac cgccggcgga ctcgcgccgt
     1561 gcctctcttc cgccgttggc gggctgatcg ctcgctattc cgagctcgca cccgaactcg
     1621 agatcattgg ttatctcggc ggctacaaag gcttgttgct cggcaatagc gtcgccgtga
     1681 ctccggaggt gcgcgagcac gccgaggtgc tgcacaagca cggcggtagc ccgatcaaga
     1741 atagccgcgt caagctgacc aacgtgaaag attgcgtcaa acgcggactc gtgaaggaag
     1801 gcgaagaccc gttgcaggtc gccgccaagc aactcacccg cgatggcatc acgattctcc
     1861 acaccgtcgg cggggacgac accaacacca cggccgccga tctcgccgcg tacctcggca
     1921 agcacggcta caaactcacc gtcgtgggcc tgccgaagac gatcgacaac gacgtatatc
     1981 cgatccggca atcgctcggc gcctggaccg ccgcggaaca aggcgcgatc tacttcgaga
     2041 atatcgtcaa cgaacactcg gccaacccgc gcatgttgat cgtgcacgag gtcatgggcc
     2101 gcaactgtgg ttggctcacc gcgtacaccg ccaagattta ccgtgagcgc ctgcgacacc
     2161 ccgcctttct gccgagcttc aatctggccc ggtcccacaa ggatctcgac gccgtttacg
     2221 tgccagagat ggagttcgat ctcgaggccg aggccaagcg tctgcgcggc gtgatggatg
     2281 aacaggattg cgtcaatatc ttcgtctcgg agggcgcctg cgtcagccag atcgtcaaag
     2341 agatggaggc caagggtgag gatattccgc gtgatgcgtt cgggcactac aagctggatg
     2401 ccgtcaacgt cggacagtgg ttcagcaagc aattcgcgaa gctgctcggg gccgagaaga
     2461 ccctggtgca gaagtcaggc tacttcgcgc gttcagcggc cgcgaacggc gatgacctgc
     2521 gcttgattca gagcatggtg gaccatgcgg tggagtgtgg gttgcgtggc gagtcgggcg
     2581 tcatcggaca cgacgaagat cgaggcggcc ggttgcgcgc gatcgagttc ccgcgcatca
     2641 agggcggcaa gcccttcgac atcgaccagc cctggttcaa cgagctgttg aacgatattc
     2701 gtcagcccaa aggcgcccat accaacgcgg gccatgggca cgacgcgcct tcgattccgc
     2761 cacccggcgc ctgatcgcga actcacgtaa tgacagcccg ggttgcgccc gcctacggca
     2821 atggcgcccc accccaagcg caatggacgc ggggcagccc gggtaacccc ttcttcggaa
     2881 cgcaatgcgc atctccggtg ccgacttcga cattctactc gaacgctgca ccgctcagct
     2941 cgggggcctg atcgggaatg ccaggcaaca cgccacctca cgggattggc tccggttgcg
     3001 cgtcctcagc gatctcggca gtggcatcgg tgaagcctgc ccgctgcccg gctatagccc
     3061 agatgacctg gtcgaagcgc agcgtagcct cgaggcattc ccgctgccgt ccctcccccc
     3121 gctcgagcag ctcgcgcgcg cccaggctcc ggcgagcgta ctattcgcgg ctctggacgg
     3181 cgctgacgct tcgctctctg tctcggcgcg attcgccctc gagaccgcat tcctggatct
     3241 gatttcgaaa agccaaagcc tgcctttgca ccgaattcta ggcgggaccg cgaccagccc
     3301 gatacccctg tccgctctgc tgcccgtcgc tcgggcttcg acggccgagt tactcgatgc
     3361 aggaagagag tgggttgcaa acggcttctc gaacctcaag gtgaaactcg ggagtagcgc
     3421 cagctggcag gatgaattcg ccgccttacg tgagctgcgc acgaccttac cggcatccgt
     3481 cgttctgcgc ggtgacgcca atcagggctg gcccatagaa ggcgcgacat cgaagctcga
     3541 ggactgcgca gccctcggtt tcgagttcat cgaggagccc gtcgcgcgtg cgcggctcgc
     3601 cgcgttgcct cgctccgccg tgcctttggc gctggacgag agtctgcagg ctgcgggagc
     3661 tgaagccgcg ttcgcggagt ggactcagcg cggagacgtc cgagtgctgg tgctcaaacc
     3721 cacgacgctc gggggattct ctcgttgttt acgactggcg gcgcgagctc ggcagctcaa
     3781 ctgggacgta gtggtcagtc actgcttcga cggtgccgtc gcccacgcgg ctgcttgtga
     3841 gctcgcgctc gcgctcggct gcacccgagc ctgcggcctc ggcccccatg cagctctcgg
     3901 ccctagtgct gaatcggctt ccttgattcc ccaactgcaa ggtgcctggc tcgtaccacg
     3961 acctgaaccg ggacacgggg tcaacgtgga tcagctggat tgacagggcc ccgagcacct
     4021 cgagcggcct gcgcgagcca taagccgccc ctatcactcg gctcacttct ggatcttggc
     4081 gaatctgggc aagccgccgc acgctgttgg agtccccatg acgctcgctt tactttatgg
     4141 agccagctcc ggcgctcttc acgcagtgac cggacccgac catgttttga gcctcggccc
     4201 agtcgcgctc aaacaacccc gttcgccctg gaagataggc cttctttggg gagtggggca
     4261 cgcgctcggt acgttggctc tcgcgctacc actggtgtgg tggtcgacgg tagccccgct
     4321 accttggctc gccgcattgg gtgatcgcct ggggggcgtt gctttgatgc tgatggcggg
     4381 ctggtcctgg tggtcgctgc gtcgccccct ttcaagcaag gatgcggaca cgcgccgacc
     4441 gtggttgatt gggctagtgc atggcatcac cggcgccacc agcctgctcc tggttttgcc
     4501 aatgctcgtc agcggctcgc cgagcctgac gctcacctac ctgctggcct tcgctctggg
     4561 cagcactttg gcgatggcta cgctcacgtc agcgctggcg cggctggggc aacgcctaag
     4621 cccgccgcta gtgacgcgcc tgcaacgcag cttagtgggc gtatcgttcg ccgtgggagc
     4681 gctttggttg gcccgcgcat agttcgagcc gcctgggcga gcgcgtcgcg gacggcatcg
     4741 cgcctggaca gcactgcgcc cgggcccttt ttacggagcg gttgctcgga tcttccctgg
     4801 gtattttgcc gctccggcgg ggcgtgagcg gcccttgctt cgagcgtacg gctcgctcat
     4861 tctgagcgcg atgatccacg cccaagccat tctgcgaacg atagctctgc tcgggctcac
     4921 gttgtcggcc ggacccgcac tcgccgccga accgaccgtg cgtgccgaga tggaatgtgg
     4981 tgcggcgtct aacgtcgtgc tgtgcgtcgt gaagttcgag ccctcgcacg cagcgcacat
     5041 tacctgggcc gaagcgtcga tcgtcgccgc tcccccgttc acttcgccgg tgctacccaa
     5101 agcgacttac cgcgagagcg cgatcaagaa ggcccacctc cgcttggtgc tgctccccaa
     5161 gggcgcgggc caaggtacca tcgtggtgaa aggacgtggg gtcgtgtgcg cgggtgatgg
     5221 cacgtcttgc ccacacttct cgaagacgct ttcggcaatc gtcagcgtcg ccggaaagtc
     5281 caagaagtag ggccgcccag ctgttcaggg gcgttctcgc ggcaggcctg cggctaattc
     5341 agtcaatccc aggttcagtt tcaggcattg ccgctggccc gatcggtact gggaagccat
     5401 tctgaggttt cggcgttcga tagtcgtgcg aagcctcccg gatcaggttg atgctcaaga
     5461 agaggccatg gtgccacccg tggtgtcatg tgctcgttgc agagatccgg gctgcgcagg
     5521 ttgcgagcaa gatcccatgc accccttgaa ggggcgcgag cggcacgggg ccaaccctcc
     5581 cctaccctgg gagagcaatg gtgggcttcc agagcgactc tggggcaccg cgttgctgac
     5641 gagcgtcgaa cctcaacgtg cgtttggaga gctcccggag ggcaagctcg gtcctgcctt
     5701 cacttttgcc gtgatcgcgg agctctatgc gatcggctcg gtcacggcct tcctgatctt
     5761 gagcgcgctg gtgatcgatg cggagttcac gcgagtcctg ctctcgaacc cgctggttac
     5821 tggtgtgacc ttcggcgtca cgctcggcct cagcgtaatc atggtattgc tgcacgcgtt
     5881 gtggggagtc acgctggagt ttgggctacg cacggcgggt aggcacgcca actacgtaca
     5941 aggcattcgt ttcggcttgt acgcctgcgg ctgggatctg ctcacttcgc cagccgggat
     6001 cgcacagggc ctgctgtctc gtggcctttg gggagcctgg ggaccgattc gcgccgcggt
     6061 cggcgtgccg cgcagcgccc tccacgcgta cgtcaccgaa tgtagaaaac tcgatatctc
     6121 ggcggcgcga cgcgggcttc gcttcagcct cgcgattctc gtcgtgagct tggttgggtt
     6181 cgcattgctg accatcgtcg ccatcgcgct ttggctgctt tgaggctgga ttcccgccgc
     6241 accactccag tctgcacggg ccgagtgtgt gtgcctgcgg cgctgcacgt cagtcgtttg
     6301 actcacgatg gtcgtgcctt tgacgcctcg acaccggttg tcggctcggc tcgagtcgca
     6361 cattccacgc gctgagccac aaagcgcccc tggatgtccc cgagttcgag cccgcgcgga
     6421 ccgtccgcac gcgatcagcg ccgcgtgttg actggaattc gagtggcgca gagcacatga
     6481 gctgacccag cttcgcagag tccgagatca gaagacgcgc tcgcacattc atattctcaa
     6541 attagacatt gaactggctc agcccttgct ccaacatcaa gttagggaaa gcgtcgtcgc
     6601 gcgaacggaa tctctcgcga cgcaagccac gcccgtcgac gatgttcgtg attcgattca
     6661 cgcgcctcgc cacgggctgg atagtcaggg gctcttcgta ggcgaacacc ttttcggtcc
     6721 aatcaccttc gaagactcaa accacactca atagagggac cggactcaac cggatttacc
     6781 ggagggagaa taccatgagc atgatcattt cctcaggcca cgttcacgga gcccagactc
     6841 acaatcacga tgagaacgcc tacgagcgga tcgacgaagt cgagcttcag cgcttgagtc
     6901 tcgagcatgc ctggatcgtg aaaccgatcc atcgcgcgag cagcgcgatc ctggatttac
     6961 cctttttcca ttgggtgcgc ggcctgaaga agccaatcga tttcaagcct gcggcggtgc
     7021 aactctatta ccactccgcg accttcccca aggtgatggg gctgatgctc gggctcacgt
     7081 cgatgcgtga aaacccgatg atgccattct attccaagca cgcattcggc gaggcagatc
     7141 accatatgat gttgatgcgt tggatgctgc agcacggcat cttgaactcg gccgccgaaa
     7201 tcgaggacgt gaccgtgagc atcgagacca atgcttgcgt caacttggcc taccagttgg
     7261 caatggaaca ggatcgggac aagtggctag ttgccatcaa cagcggtatc gagcgttgct
     7321 cgaacgactt cttcaagatt gtcgctccca ccatgcacaa gatcggtgcc ggagataagt
     7381 acttcgacat ccacgtcgaa gccgatgaac accacagcat catgggcctc aaacacttga
     7441 gcccgctaga ccccagttct cgccgggcaa agcagctggt tagcaaggcc ctcgagggga
     7501 ttagcttgtg ggctggaatg ctgcactcct ggattgggct gcaggtattt ccgcacttcg
     7561 atctcgaagg caacgtcatt ccggcccaat ccagc
//
LOCUS       DASTKU010000910         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1071637, whole genome shotgun sequence.
ACCESSION   DASTKU010000910 DASTKU010000000
VERSION     DASTKU010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071637"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /locus_tag="VFQ61_31900"
     CDS             <1..1423
                     /locus_tag="VFQ61_31900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959153.1"
                     /translation="HTLDNGWRSADDFLAQFGPLELMRGLDGAPELRGIILTKAVGFH
                     EKIARKKTAESAADDLRIALEENVTSPADILALFPTDDRIRYLDRQKLFAFAVDDGFF
                     AAPRVGEEQERALDRLMYLIETALGEGLLTFADFADGVTFETISASLAPDDLRRLLTG
                     ALTHGRAGEPLTEERLLAIVPLRTVVGRISLEVTWRNVVLAKVARPAGLVSGRASANS
                     PSAAPETTPSRTPAAIAPSLRGSNGKSKAPPPAPPIITAPAVSVAAEPVITPNAVNVA
                     PPSSPAPSPASAPISVDAPIPLVDPFADIESEVVIEAAEGPPSEANEELAARAKVVER
                     LTNINRLPPRHADLSIQLLFAIDSMYADLASAASDEEREDAVRDAFPNHQHAAVALLA
                     LLELLEPSIKMDPDVAKADIDALVNVFLIEERRYQRERSEPQKSAATPSTTPAPGTAR
                     RGAPPPLPRGTPMPPPLPSEKGR"
     gene            1595..1678
                     /locus_tag="VFQ61_31905"
     tRNA            1595..1678
                     /locus_tag="VFQ61_31905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1629..1631,aa:Leu,seq:cag)
     gene            1858..3489
                     /locus_tag="VFQ61_31910"
     CDS             1858..3489
                     /locus_tag="VFQ61_31910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959154.1"
                     /translation="MFRSSKIPSSLLVLALSCSSASPVGEGGATAGAGGSGALPSAGA
                     AGVGGATGGKAGASGANTAITGGVSAGMGSATSGAGTATGGLPAAGSFSGGAGGLTTT
                     AGGLTGLSGAGGKVATSAGGTGAGGRGEVSGGSSALSGGRPSMGGTAGTLGGSSVGGA
                     MGGSAAGGAPATGSAYSCDGTTAGYDVVATQSGSTWTVSQGSRQVYSGSDFQQVLTRA
                     YASLSSGRTTKQSILIQGSGDIPASAQVAIPSYTRLNVCGTVNVTGTASGSDRSPFYA
                     RNARDIEISNLKMTGAPQYGIFFRQTNNVILGHIELRLSSSAGIGVRVDSGPSAQSQT
                     TFNENLTIDYVYGSGMGSHVVETYGISNIRIGTIEGSAVGECGLLLNRSIHAEIGLVS
                     CSDCATGTGYAAFRVANSVGKVGNDFPSGDIHVGKVYARGGGRGIFSVSGSGGLTIDE
                     IDIANTGSTPILLQNAYNTIIAAKAGTVSKGLVQLSNDTDNTNEGVYPPSKNVKLQNL
                     VLSNGASVRQDWCAQFGSNGCSAANITGGTVSMCQ"
BASE COUNT          666 a         1112 c         1144 g          654 t
ORIGIN      
        1 gcacactctg gataacggct ggcgaagcgc ggacgatttc ttggcgcagt tcggtccgct
       61 cgagttgatg cgcggattgg acggcgcgcc cgaacttcgc ggcatcattc tgacgaaggc
      121 ggtcggtttt cacgagaaga tcgcgcgcaa gaagaccgct gagtctgcag ccgacgactt
      181 gcggatcgcg ctggaagaga acgttacctc acccgctgac atccttgcgc tgttcccgac
      241 ggacgatcgc atccggtatc tggatcgcca gaagcttttt gctttcgccg tcgacgatgg
      301 cttcttcgcc gccccccgag tgggtgagga gcaggaacgt gcgcttgatc gcctgatgta
      361 cctgatcgag accgcgctcg gcgaagggct gctcacgttc gcggacttcg ctgatggcgt
      421 gaccttcgaa acgatttcgg cgtccctcgc gccggacgat cttcgccggc tccttactgg
      481 cgcgctcact cacgggcgcg cgggtgagcc gctgactgag gagcggcttc tcgcgattgt
      541 accgctgcgt acggtcgtgg gccgtatctc gttggaagta acctggcgca acgtcgtact
      601 cgcaaaggta gcgcgccccg ccggcttggt gagcggccgc gccagcgcca acagcccgtc
      661 cgcagccccg gaaaccactc cttcgcgtac ccccgccgcg atcgctccct ctttacgggg
      721 ttctaacggg aagtcgaaag ctcccccgcc cgctccgccg attatcacgg ctccggcggt
      781 gtctgtcgcg gcggagccgg tcatcacgcc taacgcggtg aacgtggcgc ctccgagttc
      841 ccctgcgcca agtccggcgt cggcccccat ctcagtagat gccccgatcc cactggtaga
      901 cccgttcgcc gatatcgaga gcgaagtggt gatcgaagcg gcggaggggc cgccaagcga
      961 ggccaacgaa gagctcgcag ctcgagccaa ggtcgtggaa cggctcacga acatcaatcg
     1021 tctgccgccg cgacacgctg acctctcgat ccagctcctg ttcgcgatcg actcgatgta
     1081 cgcggatctg gcgagcgccg cgagtgacga agagcgagaa gacgcggttc gagatgcctt
     1141 cccgaaccat cagcacgcgg ctgtggcctt gttggccctg ctcgagctgc tcgagccttc
     1201 gatcaagatg gatcccgacg ttgccaaggc tgacatcgac gcactggtta acgtctttct
     1261 gatcgaagaa cggcgctacc agcgagaacg ctccgaacca caaaagtcag ccgccactcc
     1321 gtccactacg ccggcgcccg gtacggctcg tcgcggtgcg ccacctcccc tcccccgcgg
     1381 cacccccatg cctccaccct tgcccagtga gaagggtcgc taagacggta cccaggaaac
     1441 ggccgaccga acttcccccg cgacggtttt ctagcacgcg atcttcacaa tccctcttgc
     1501 cgaaggtcta cacctcgagc tagaaacccg tcgccttcgc cgccggggaa ccccggtcgc
     1561 aaaggcagca aaaagactga taaaagcagt caccgcgaag gtggcggaat tggcagacgc
     1621 gctggattca ggttccagtg gggttactcc tgtgagggtt caagtccctc ccttcgcacc
     1681 gtaagttact cgagggctcg agtactgaat gattggcccg ccggggaggg tcagagtcgt
     1741 ttacatcgat cgctgtcgag tcgacgtcga gcgctgtcct tttggcgttc aatcggtgtt
     1801 ctcatcgagg gcggcttcgg gtgacccgaa tatgcgcggt cagagtactg accagtgatg
     1861 tttcgctcat ccaagattcc gagttccttg ctggtattgg ctctcagttg cagcagtgcc
     1921 tcacctgtgg gtgagggcgg agcgacggct ggtgcgggag gatcgggagc tttgccctcg
     1981 gcgggagcag cgggggttgg tggcgcgact ggcggcaagg cgggcgcatc cggcgccaat
     2041 accgcgataa ccggcggtgt cagcgccggc atgggaagcg caaccagcgg cgcaggcacg
     2101 gctaccggcg gactgcccgc cgccgggagc ttctcgggag gagccggcgg gctcaccacg
     2161 actgcgggcg ggctgacggg attgagcggg gcgggcggaa aggtcgcaac cagcgccggc
     2221 ggcaccggtg cgggtggtcg aggcgaagtg agcggcggct catctgcact atcgggcgga
     2281 cgtccgagta tgggcggtac agccggcacc ttgggaggct cgagcgtagg cggcgcgatg
     2341 ggtggctcgg cggcgggtgg agccccagcc acgggcagtg cctatagttg cgatggcacc
     2401 acggctggct acgacgtggt cgcgacgcaa tcgggctcca cctggaccgt ttcgcaaggc
     2461 agccgccagg tgtactcggg ctcagatttt caacaggtcc tgacgcgcgc gtatgcgagc
     2521 ctcagctccg ggcggaccac gaagcagagc attctcatcc aaggctcggg tgacatcccc
     2581 gccagcgcgc aggtcgccat ccccagctac acgcggctca acgtgtgcgg cacggtcaac
     2641 gttacgggca ccgcctcggg ctcggatcgc tccccctttt acgcgcgcaa cgcgcgcgac
     2701 atcgagattt cgaatttgaa aatgacgggg gcgccgcaat acggaatctt ctttcgtcag
     2761 accaacaacg tgattttggg gcatatcgag ctgcggcttt cgtcttcggc aggaatcgga
     2821 gttcgggtgg acagcggccc ttccgcgcaa tcccagacca ccttcaacga aaatctcact
     2881 atagattacg tctatggatc cggcatgggc agccacgtcg tggagactta tggcatcagc
     2941 aacataagaa tcggcacgat agagggcagc gcagttggtg agtgtggtct gcttttgaac
     3001 cgcagtatcc acgcagagat aggcctcgtc tcttgcagcg attgtgcgac gggcacgggt
     3061 tatgcggctt tccgcgtagc gaacagcgtg ggtaaggtgg gcaatgactt cccgtcgggc
     3121 gacattcacg tgggcaaggt gtacgcgcgg ggcggcgggc gcgggatctt ctccgtctcc
     3181 ggaagcggtg gcctcacgat cgacgagatc gacatcgcga acaccggtag cacgccaatt
     3241 ctgcttcaga acgcatacaa cacgatcatt gccgccaagg cgggcacggt ctcgaaggga
     3301 ctagtgcagt tgagcaacga caccgataac accaacgaag gtgtctatcc gccctcgaag
     3361 aacgtgaagc tgcagaatct agtgctcagc aatggcgctt ccgtacgtca ggactggtgt
     3421 gcgcaattcg ggagcaacgg ctgcagcgcc gccaacatta ccggcggcac cgtctccatg
     3481 tgccagtaag ggcttcactc gcagggtcgc tcattcttgg aagcaacggg acgccaagtc
     3541 gtgtctctct tgggtaagtc tttgaacgcc cgacac
//
LOCUS       DASTKU010000911        45704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1071794, whole genome shotgun sequence.
ACCESSION   DASTKU010000911 DASTKU010000000
VERSION     DASTKU010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 45704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45704
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071794"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFQ61_31915"
     CDS             <1..360
                     /locus_tag="VFQ61_31915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959155.1"
                     /translation="VINGILNVEAVRIAQAKYGKRALTGEETRWGFEHLDINEARLKE
                     LGAEGLLQPIKLSCEDHEGGGRVRFQQWDGEKWKLISDWVAADRPLLRPIIEASAAKY
                     AQEKGIKIRDCSADSEK"
     gene            400..1200
                     /locus_tag="VFQ61_31920"
     CDS             400..1200
                     /locus_tag="VFQ61_31920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9959156.1"
                     /translation="MTDSTPLLELDQLEVAYDGIILALRGVTLAVAPGSVVALLGANG
                     AGKTTTLKAASGLLSAERGEILRGRLRYRGRDSAATPARVLVKQGLVQVLEGRHCFPH
                     FSVEENLIAGALVKRPNRAELARKLEHAYQRFPRLKERRRSLAGLTSGGEQQMLAIAR
                     ALMAEPQLVLLDEPSMGLAPLMVQEIFEIVKSLNELDGVSFLLAEQNATLALRYSQHG
                     YVLENGKVTTSGPSAELAKRDDIRESYLGLKNNGARRPRSLRPTNIVT"
     gene            1270..2595
                     /locus_tag="VFQ61_31925"
     CDS             1270..2595
                     /locus_tag="VFQ61_31925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959157.1"
                     /translation="MRSSLALVCVLEALTIDGLASAQDAATPPNAAAAPTPTGTTTEP
                     APPAPVVTNPNSPAPPGAGVFPNSPAPDVNAPRTNDAASSNAAGASTKLNATSDVAPA
                     REAASSWTEKLKLGGGATLWYYQPTVDGQKNNLDFFNTRLTIDADFGDGFGFYLEPRI
                     RDTKLRSFFAGTAWIEQGYASYRFGDHKLKVGKVYSQLGLFWDNSFWGNVQVYAGLKL
                     APDYGASLEGKFEVDPAWGVGYAAQFFLIDGSTNVSLPGRDTISVPDSRRRNQVVLRA
                     EPYLNFAKDGVAKVGLSTQHFDADSPAIADGSVWRYAADLKVALGGFGVWGEYLRQQG
                     QSVSAYPIEEIPATDTEGAVPGRAAKKIDYYLAGAEYTYGRFTARYTFSAARYAGLKV
                     KEWMHVPALGFKVNDNLQLGAEYVHWTRSFSGNHDKYNRSLNVLVFVKF"
     gene            2626..3150
                     /locus_tag="VFQ61_31930"
     CDS             2626..3150
                     /locus_tag="VFQ61_31930"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9959158.1"
                     /translation="MSKQVVLISGSPSHPSRSGTVLDWFGRALREAGWQVETFTLDDF
                     EPAALVRAQAEDPAVTRVRRAVDSAAGVVLSTPVYKATYAGALKLIVDLFDPKALQGK
                     ALLGIGTARIPEHLSEVEVSFQRLAQFFQGSVHVPGLGLLDADLGASPNYALTQTGRV
                     AFEAALARYLEALP"
     gene            3147..3713
                     /gene="ribD"
                     /locus_tag="VFQ61_31935"
     CDS             3147..3713
                     /gene="ribD"
                     /locus_tag="VFQ61_31935"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET9959159.1"
                     /translation="MNTPPSVARTKPAWVWGETARRADARHDRFMARAIGLAQRGRPA
                     PNPHVGAVVVRDNRVVGIGHHELAGGPHAEVVALRNASWLARGATLYVTLEPCNHFGR
                     TPPCVEAILQAGIARVVIGCSDPNPRVKGGGAQRLRAAGVVVIEGVQQPEALRLIQPW
                     LATLNFASRPPYSALASHSTLDAPSEGG"
     gene            complement(3717..4559)
                     /locus_tag="VFQ61_31940"
     CDS             complement(3717..4559)
                     /locus_tag="VFQ61_31940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959160.1"
                     /translation="MATGRFDWSIRSFAFALLAMAQPACSGESQVAPLSDTAELCDGS
                     SSIRLSYTNAGGGPLPESYGFTTPYGHEFFTVDGQCNYWVGRDFESGTQYGRLSSAQA
                     KKLSQNLLYGRFAAYAEFRGEACPDASTRTLYDGKSRLSCTCSCDGPAEYRAAFESVT
                     SVMTELWDAGQGSAPALRVLAQRVDGSVEDLEGEAWPSTLQTQLGEGALEPDAPASAF
                     SEDAGQTVEIESDVQALRAQREQGVGGLSLVLEEEVTYRVYARDLLPGRASQALRANL
                     NSRD"
     gene            complement(4590..5645)
                     /locus_tag="VFQ61_31945"
     CDS             complement(4590..5645)
                     /locus_tag="VFQ61_31945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959161.1"
                     /translation="MEFPEPSELRPVVVGYAQVLAQLELEPGEQALVLPNGEWFPDKF
                     TGDADSLSLLVARMQGYAGLEEIEIETALSGETAEGSSCGTGGCGSGGCGTSSTKKVE
                     APTLTKTARGYLIEVNASAMSHPIAFTAAIARMLGHVRLLEAGKDDPNAKETGVEAAE
                     LAATQLGFGVLLLEASYLYSKSCGGPSVGKATVLSCPELALALACFLDFEGQKPRAAL
                     AELATTQKAALDEAWTLVQSNPELIQKLKSHPNAVASGKFQLKESRSWLSRLFGQKPS
                     RPKDSAQAALEALERGDSLEHVEALLGKEAPAVTPRSAKTPRAEDDLRDLIDEALAET
                     RAEREREELASAGRSRY"
     gene            complement(5854..7041)
                     /locus_tag="VFQ61_31950"
     CDS             complement(5854..7041)
                     /locus_tag="VFQ61_31950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HET9959162.1"
                     /translation="MKKLTDYVVFNDRTLAKRYANSPIPMSVLYEAYFDGAIDIPGNL
                     YELLDERNRFVKYTLTPEHAKWAVTNFLPEVTIHSKSQDKRIVREHYDRGNDFFGWFL
                     GERMVYTSGYFTDPNQTVEEAQDNKMHLVCQKLRLKPGERLLDIGCGWGTLARHAAKY
                     YGVDATGVTISENQTEFGNRRIREWGLEDRARILCCDYRDIPYQKFDKIVSLEMVEHV
                     GVKNLKKFYSWVSELLKDDGLFLLQWTGLRRELSGETLIWGLFMNKYIFPGADASLPP
                     AGMLKPAEEVGWETFTVENVSQHYGWTIRRWHDYWMSNEQSIKAVYGERWYRIWHFFL
                     GWSVCIAGQGNASCYQVVFNKNLDGFDRTQHILDKGVVHGARETNIQAPRTTPGNGAF
                     AAE"
     gene            complement(7283..8176)
                     /locus_tag="VFQ61_31955"
     CDS             complement(7283..8176)
                     /locus_tag="VFQ61_31955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9959163.1"
                     /translation="MNFPAMNPELARYLREAHEAPRLSREEELILARNIKATGDRQAQ
                     DALTRAHLRDVVAIARKYQRYGLPLEELIAEGNFGLVQALGKFDPERGVRFVTYSAHW
                     IRAYVLNHIINAWSLVGAGSGPLRSRVFFKLRRERTRITNLLGPGEEAERALAQRMGV
                     SPETMQRMLQRLEARDVSLDAHVGEDAQVRWLDLLPSEEDQEETLFEHELGDNLQTTV
                     RSALTVLDSRELYIAERRLMADQNEELSLAEIARTLGVSRERARQLEARAKRKLRTRI
                     ARQGNAIVHEWLTLRSAAPAA"
     gene            complement(8313..8909)
                     /locus_tag="VFQ61_31960"
     CDS             complement(8313..8909)
                     /locus_tag="VFQ61_31960"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET9959164.1"
                     /translation="MHSHGCDNEWGWHMSRGFGGRGGRFAGPPRGGRPWWAEAIGEPP
                     PARAERGGVRYLVLEAIEQQPRHGYEIIQHIEQRAGSAYRPSPGVIYPTLQMLEELGH
                     ARVIEQDGRKVYAITDAGKQDLEANRQAVTDFYSRFDADSWESYTDDFGELMKFFGRL
                     MRTVKLGMHRGRLSPSKMRAIRLALEEALQKINDTLNE"
     gene            9146..9219
                     /locus_tag="VFQ61_31965"
     tRNA            9146..9219
                     /locus_tag="VFQ61_31965"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9180..9182,aa:Arg,seq:tct)
     gene            complement(9730..10620)
                     /locus_tag="VFQ61_31970"
     CDS             complement(9730..10620)
                     /locus_tag="VFQ61_31970"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9959165.1"
                     /translation="MAGQLNYHHLRYFYAIAKAKSMRQAAEQLRVSPPALSAQLKQLE
                     TLLGHQLIERAPSGPTLTEAGRIAFDYAETIFRAGDELQDVMTHGLTRGRRVVRVGAV
                     ATLSRNFLIGAFEPLLARSDIGLVVRSGSLSDLLTLMQEHEIDIVLSNQPAPGGTRVS
                     FHSRLLAEQPVSLVGGVHWKRREFRFPEDCAAVALTLPGPGSNLRAAFDAAMDEAGIR
                     PLVAAEVDDMAMLRLLAQETEGLTLVPRIVVKDELERNSLIEKRRLPRFNETFYAISP
                     SRRFPNPLVRELVEHVATRG"
     gene            10694..12265
                     /locus_tag="VFQ61_31975"
     CDS             10694..12265
                     /locus_tag="VFQ61_31975"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET9959166.1"
                     /translation="MSWSKAFLLFIPLLPALAGALPLRRPKTAGVVTSTACGLAASAA
                     AFACVQAYFSPSLTTLRWSLTSTSPISLGLQLDRLSATMALLITLVGCCVLRFAARYL
                     EGDAEQVTFLRRVSLTIAAVAGFALAGDLLVLLGAWILASLVLHRLLTHYPQRPAGVR
                     AAKAKFLVSRLAEGAVLCASVLLYRSFGTLEFETLFLRLEQASPELLQWPALLLVCAA
                     LLKSAQFPFHSWLPDTMETPTPVSALMHAGIINAGGFLMIRMSPLLGQVPIATGVLAL
                     AGATTAAFGALVMLTQPSVKRALAYSTIAQMGFMLLECGLGLFGLALLHIMAHSVYKA
                     SAFLRAGSAVNAAPRSLVPLDSRAILASLTVATGLVLGASRVGHSAHSLGAVVMAVIL
                     ALSIAYGLTRYWAVSERRFAWMGLSYAAAFTGAATLVHSLGATLVPARSEVYVAPWLA
                     LLLCTLFIGLFAIQVSLWRMARHALGRALYVHALNGFYVSALVNRALERWWPPRRYEL
                     VRSIEPSLAQRMTQS"
     gene            12262..14661
                     /locus_tag="VFQ61_31980"
     CDS             12262..14661
                     /locus_tag="VFQ61_31980"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HET9959167.1"
                     /translation="MNTEMREPSQCGEDVNAAHEAAVREALARIAPLFPSRSFVAVNP
                     FLGFTNLPFEEAAWRVMTLRGRAPLLDANEYRKLFAKGEITIEDLDWVTDASWSVAQL
                     LTALESPVDHAIDVPWTAVDALESERAEERWRDFVVEEISKWCAVYYDQNQTTWTLPW
                     ARAGLYSVFREAARFDRAPDTHGLHTLRDLVATLPPDAEAAIAVILRELAPPSGREAD
                     FVYGQLALVSGWAGYCQYLVHEHRLKGDDSPALRELLAFRLAYDLALLRMRPNLRSSF
                     GVRRRSVTTAPLAALCRWQLAYEVAWQRAVLRKLTQAHDPLSPTEPKRPRAQAVFCID
                     VRSEPLRRHLEGVSEDVRTLGFAGFFGFPLSHERPDGSGAEARCPALLAPTAESAECG
                     VRRHDRAVRARARASTSKAIQNAAASCFSFVEAAGLAFLAQLWSQTDTSGPAWLGTPK
                     PALLDFEHSDLDALAVRAEATLRNMSLTRNFGRLLLLCGHGSHSANNPFASGLDCGAC
                     GGHAGDVNARIAAQVLNHPGVRQRLAGRGIELPEDTWVLAGLHNTMTDEVTLFDLDLT
                     PESHRADVDALLRSLELAGAATRRERAVKLGHATDEDLLYALRKRAQDIAQTRPEWGL
                     AGNAAIIVAPRQRTCGLDFEGRTFLHEYRHEDDQDGRMLQLILSAPVVVASWINLQYY
                     GSRVDPVLYGAGNKVLHNVVGGVGVFEGNGGDLRVGLPLQSIHDGERFIHEPRRLSVF
                     VEAPREKVWRAMASLPDVRKLFDGRWIHLLTIEHGEVYAYEPSGFRKLESTGTRQSVA
                     A"
     gene            14867..15211
                     /locus_tag="VFQ61_31985"
     CDS             14867..15211
                     /locus_tag="VFQ61_31985"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9959168.1"
                     /translation="MPKYLCLQRSLPGKESSSDKPSPAQMQEMYEKFNAWREKFQNNL
                     VDLGGRLGEGRLVSPEPAPDGPFAEVKEIVGGYMIVSASNFEEAIAIARQCPGLVRAG
                     SGVEVLEIRSPG"
     gene            15208..16440
                     /locus_tag="VFQ61_31990"
     CDS             15208..16440
                     /locus_tag="VFQ61_31990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9959169.1"
                     /translation="MTSALGEHFFRHEFGRLVAMLSRRAGVQHLELVEDAVQVALLAA
                     VESWTRTFVPANPSAWLYRVAHNHLLGELRRRTRRDELTGSLVEHSSSSIEEPAVLLS
                     GDVPEAQLRMLFVCCDDSIAIESQLMLALRTLCGFDISEIAQRLFTSETNVYKRLGRA
                     RSRLRETRLVTDEMSTEQLASRLPAVHAILYTLFTEGYLSSHAEYAIRRELCDEARRL
                     TALLAAHPVAATPETFALLALMHLHIARMSARQDGSGGLVLLEEQDRSQFDREEIVQG
                     LSWLARSATGDVFSRYHAEAGIAAEHCLAPSFSETRWDRIVECYELLDRLAPSAIHTL
                     NRALAVAECTNAAAGLAVIEGIEPPSWLTGSYLWSAVLADLNRRGGRNELAKSYRDTA
                     IELAPSPAIAACLRRRLA"
     gene            16591..17526
                     /locus_tag="VFQ61_31995"
     CDS             16591..17526
                     /locus_tag="VFQ61_31995"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9959170.1"
                     /translation="MKLISDAPVPSPGRGEVLIRVAAAGLNFADISRAYGTFRNGPKA
                     PYLAGFESSGEVVALGEGVTSPQVGTRVVGVGYGAFAEYAVLYAAAAVPVPVSWTAPQ
                     ALGLVVNWPTALAALRPLGRIAAGETVLIHAAAGATGQAAVTLAKHFGARVIATASPS
                     KHETVRALGAEHILDSRAEDLPTQVKNLTSGAGADLVLESTGGSTFKASLSATRRVTG
                     RVVVYGLAGGEAALSNWELVYEQQVQVIGLNIGVLSQAAPQIFGTVVGELLELIATGV
                     VTPTSPTSYALADGSKAIAALESRATIGKLALLPG"
     gene            17574..18569
                     /locus_tag="VFQ61_32000"
     CDS             17574..18569
                     /locus_tag="VFQ61_32000"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HET9959171.1"
                     /translation="MTKRSLETEAQDRFAAEMGRYRRELLAHCYRMLGSSREAEDSVQ
                     ETYLRAWRAYGSFERNASARSWLYKIATNVCLTALGSRRIRVLPSGLAGPYDGPERLA
                     DIVPPGEVSWLEPAPDAWLDATTDEPDMLIEARESLRLALIASLQYLPARQRATFLLR
                     EILAFSAEEAAEILGTTTTAIKSGLQRARATLDQRMPEREALLEPTDSRAIELLAGYI
                     AAFERSDASLLLQVLRADAALEATPFRDWVTGRVKCIQLLQNYVLGTPGDWRMLATRA
                     NGQPAAALYRRDENGALRSYGVVVLAPTPAGVSRVIAFHDPALNRLFGFQDTFEG"
     gene            complement(18613..19038)
                     /locus_tag="VFQ61_32005"
     CDS             complement(18613..19038)
                     /locus_tag="VFQ61_32005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959172.1"
                     /translation="MTKRIAIGALCYLVPTFALGFVWHLVAFKGYYDALEIYRKDVII
                     PFGFLSMSIQALLFAWVYARVFARWPDLKSRTLGYAAFGALLSWSFTTIAVAAKNVMS
                     SVPDYLLIESGYTFVQWLFVGPLTVLLLRRTEQLQATHP"
     gene            complement(19035..19493)
                     /locus_tag="VFQ61_32010"
     CDS             complement(19035..19493)
                     /locus_tag="VFQ61_32010"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9959173.1"
                     /translation="MKPSERAEVRRHLAATPERVFAAFASAEVVGQWLTPDPEVKLSV
                     LELDFREGGRYRFVYDTPGGQRMEVGGVYREIEAPCRLVFSWLIEPPDIHAGIESRVT
                     ISIAPNAEGSDLVILHERFERADANARHAEGWRGAVDQLQRLLASRRQSS"
     gene            complement(19490..19819)
                     /locus_tag="VFQ61_32015"
     CDS             complement(19490..19819)
                     /locus_tag="VFQ61_32015"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9959174.1"
                     /translation="MQSAAALDRTFHALSDQTRRSMIHALAGGVARSAGELGKQFNSA
                     QPTISRHLKVLEQAKLIERSIEGRVHRFRLRQKPLQDAGAWIERHQRFWHGAIDQLEA
                     LLGRDTP"
     gene            complement(19830..20738)
                     /locus_tag="VFQ61_32020"
     CDS             complement(19830..20738)
                     /locus_tag="VFQ61_32020"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9959175.1"
                     /translation="MTTKHRTKIAFLGLGAMGARMATRLVSGDIDLQVWSRSGVPKEF
                     TSLASHLVPTPEAAVAGADMAIAMVTDDPASRGIWLDSGALRGMRRGAVAVECSTLTP
                     TWVAELARHARHAGVAFIDAPVVGSRPQAEAGNLIFLAGGDASALDTVRPTLLRMGSA
                     IHHLGASPAGALSKLLANALFTTQVAAVAEIIALARRGRLDLPTLARTLEALPVMSPA
                     AKGAMTGMLAGGFTPMFPISLATKDLGYAVAEGTATRLHMPMTSAARDVFARGLESGL
                     SDENLTAVVKLYAAGHAGLDERKPKI"
     gene            20825..21727
                     /locus_tag="VFQ61_32025"
     CDS             20825..21727
                     /locus_tag="VFQ61_32025"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9959176.1"
                     /translation="MDRLDALRLFSRVAERGSFSAAARELKVKQSTASKWVAELESTL
                     GSTLVERTTRSIRITESGQRLLVRAREVLAAYDELSADFENRAPTPRGSIRVSLPVVF
                     GRLYVLPAIADFLKCYPQVDAEIVMSDRYVNLVEEGFDLALRVGVPTDTSARGRKIAE
                     SRRVLVAAPAYLRAHGTPRTPEDLRRHQCLVHGDANVPGTWRFGRAGESGVPISVKGR
                     VAANNSEAVALMARRGLGLALLADWMVAEDIRRGRLVPLLPEYALPPAPVYALSPPGR
                     FSSTTVGALIDHIAQVVPARISRA"
     gene            21796..22767
                     /locus_tag="VFQ61_32030"
     CDS             21796..22767
                     /locus_tag="VFQ61_32030"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9959177.1"
                     /translation="MDVLADVLSITRLGATVVAQAELVPPWGLEIDPIAETHVHVVLR
                     GSCWLRTTGDRRPIRLGSGDVVLIRAGVGHSICDDPKTKPAPHGDVLAAMPLRLAALP
                     ASRAKETTLVLCAKYLFQGVGAPGGAHPLTSLLPPLIHLPAYEAERDKQLQLLLQLLG
                     HEARAAGVGTELVVPRLVDSLLVFVVRAWLAGQPLAAGGWFAALRDPHISAALGLMHG
                     RPEIAWSLEQLAREVGLSRATFARRFLELVGETPVAYLTRWRMCLATKLLRETSLSLE
                     EIAPRVGYQTAAAFSRAFRRSLRTAPGAYRAEARAQGQLAHLAPALS"
     gene            22948..23235
                     /locus_tag="VFQ61_32035"
     CDS             22948..23235
                     /locus_tag="VFQ61_32035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959178.1"
                     /translation="MFIVLLRFSANIDQAGDLMMAHNEWIERGLEEGIFLLVGSLQPK
                     KGGAILAHGTTRSALETRVLQDPFVAHDVVSAELLEISPSKTDPRLAFVLG"
     gene            complement(23287..23838)
                     /locus_tag="VFQ61_32040"
     CDS             complement(23287..23838)
                     /locus_tag="VFQ61_32040"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET9959179.1"
                     /translation="MDTSKRRQVGERLPSLQFSSLHGETVGVPNPNASIRLIHLQFRR
                     FAGCPVCNLHLQSFARAHGRISNAGVLTVAFFHSRSEQMMPYQGQLPFPCVADAERRW
                     YRYFGVERSAAAVAHPRVVGAALKGLFTAPSNPFVGGWDQTGLPADLLIDRSNVVVAA
                     RYGAHANDQWSVDELLELAGTKA"
     gene            23925..24899
                     /locus_tag="VFQ61_32045"
     CDS             23925..24899
                     /locus_tag="VFQ61_32045"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9959180.1"
                     /translation="MNVDPGALVALNVLLEERSVTRAARRLGITQSSMSHRLARLREA
                     LGDGLFVRVGATLTPTPRALAIAEPLADALRALNAAVAIPLAFDPGRANGRASIVMPD
                     LLAPFAPDLVARISAQAPGLALRFASVSGELSTALAGPELSLALVPARFAAADMQSRA
                     VGELRFGVAGRRGHPALARRLSLERWLAERHVVVSVGLDRSNVIDDELNRRGLERRVG
                     LVVPSFLAGLFSLAQSNLLMNVPMPLVDEAAKQLGLCVRDAPLPLPRLHFSMVWHPRF
                     QHDAAHLWLRQQVLELVQHAFRKQPRADSNTKSRSRVERTTSRSNGGT"
     gene            24967..25695
                     /locus_tag="VFQ61_32050"
     CDS             24967..25695
                     /locus_tag="VFQ61_32050"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET9959181.1"
                     /translation="MRIALVSDLHGNALAVEQVLNRIRFDGADQLICLGDVATLGPEP
                     ERVMELLHESGATCILGNHDEFMLRPETVAEYTTISVLIDAIDWCRANLSAASLEFMR
                     SFRERYEVPLNAQEVLLAFHGTPASNTTDLLSTSDPTPVEEWIGAVPGSVLVGGHTHL
                     QMLRQHRGRLLVNPGSVGMPFREYVGGGPPEVLPFAEYAVIDSDSHSVEVRLQRVNLD
                     VTLLRGSVLAVDYPLASFLVKAYS"
     gene            complement(25795..26034)
                     /locus_tag="VFQ61_32055"
     CDS             complement(25795..26034)
                     /locus_tag="VFQ61_32055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959182.1"
                     /translation="MIKHLIGISLTCFALGLSATACTANVDDPVIDQDGQDGDKDDCV
                     TKCDSTKTTCVGSCTDDGCRGSCETKHEDCVSSCD"
     gene            complement(26357..26845)
                     /locus_tag="VFQ61_32060"
     CDS             complement(26357..26845)
                     /locus_tag="VFQ61_32060"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9959183.1"
                     /translation="MESGQVDWDPASFPTFWVNQASRLLMRRFEDQLRPLGLSMAYLH
                     VAITLERNGPLQQKALLKFIHVEQPTMAALLKRMERDRLVTRKPDPNDSRAQLVTLTS
                     RANTALARAKAAMSDVVTRAMAGIPEPDQTRLVKTLRLMLENLADDPSQLTGLGMAID
                     RS"
     gene            26933..28183
                     /locus_tag="VFQ61_32065"
     CDS             26933..28183
                     /locus_tag="VFQ61_32065"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9959184.1"
                     /translation="MKTSIINVRVFDGESVRPEASVHLFEDRILGLGGQAPSDSEIVD
                     GKGGTLLPGLIDAHVHTDESGLALALQFGVTTELEMQGVNTRNDRMHITHDDTLADVR
                     SSGFALTPPGGHPSELFPEDFDPSRLPRRRERERVSPLVQAKVSTPREAETVIPELIA
                     GGSDYIKFMIDDGCVEGHPGLPMLDYATLSAGVREARKRGMLTLAHALTLDATRMAVE
                     AGIHGLTHLFMDVPHTREVVDTIARSGAFMVPCVVLNASMMGINGASLADDPRVAPHL
                     NEAWDATLRSSFNHYPQGKFEDQMASVKALHDAGVELLVGTDVSQPFPFLGGLAHGAS
                     VHHELQYLVQAGLSPIQALRAATASTARRFGLLDRGRIAPGLRADLVLIEGEPTRQIS
                     DTLNVRSVWRRGTHAVNRPSSCSW"
     gene            complement(28952..29656)
                     /locus_tag="VFQ61_32070"
     CDS             complement(28952..29656)
                     /locus_tag="VFQ61_32070"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HET9959185.1"
                     /translation="MRRNDRLFAIIQVLRGRKQPVTAQRLADEFSVSLRTIYRDMAEL
                     SAQGVPVRGEAGTGYVIEASYELPPLMLTADEVEAAMLGLMWVFQRGDSALSHGARSL
                     LEKLSQVIPRELRPLLFDASLRPALARTRFEDATDLGPIRTAIRERRKIEIAYQDANG
                     KLTRRTLWPLLLAYMDEVRILVAHCEEREAFRHFRTDRLREVRVLDVKYPLSFAALRK
                     RWAEQFPEREHGTLLR"
     gene            29761..30165
                     /locus_tag="VFQ61_32075"
     CDS             29761..30165
                     /locus_tag="VFQ61_32075"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9959186.1"
                     /translation="MQFASTRIITQDVQRLVGFYQQVTLIAPVWRTPEFAELSTPTYT
                     LAIGSESTVERFYPGAARGSRNQSTILELRVADVDVEYRRLAERLDVVVQVPTTMPWG
                     NRSLLFRDPDGNLVNLFTPVSPEAIQRHTRGT"
     gene            complement(30299..31048)
                     /locus_tag="VFQ61_32080"
     CDS             complement(30299..31048)
                     /locus_tag="VFQ61_32080"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HET9959187.1"
                     /translation="MFRLHMRVAPYHEFEALPFGSSTQLFVLTGAGISAESGIQTFRD
                     SNGLWEQYDVRQVASPSGWAQNPALVWRFYSQRRAQARETRPNAAHVALAKLQARWEN
                     QVFLCTQNVDPLHEHAGSPAVVHMHGELFKSRCEREHAPPFLDESLYLNSDQLPRCDC
                     GAGIRPHICWFGEVPFFMGEIERALSRCNLMVTIGSSGSVYPAAGFVAAARANGAKTV
                     YVGPERPENATAFDECRLGTATEVVPSLFES"
     gene            complement(31355..32713)
                     /locus_tag="VFQ61_32085"
     CDS             complement(31355..32713)
                     /locus_tag="VFQ61_32085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959188.1"
                     /translation="MNPKKHRHILAQRVFGLGIFTLAAYAGCLATPRTEPRDDGSSGS
                     SGTPSGGGSANASGTGGKSGNTGGGNGGAVDDAQGGTSEGGTHADSAGSIGNGGEDST
                     STGGTVEGGAAGMASGGGAGAGGATYNECATGLVTCAGSPECTTDLTVGNPSGKTVMD
                     CGACGVSCSVNHASSSACSNGICEPACSTGFADCNAEAANDGCEANLASPASCGACDH
                     TCATNGVAAASCTDSKCVPACAPGFLDCNVDDGVRRDDGCEVFGNSLLACRADCASSG
                     TACASNQVCNEGSCVAPEGLVKLTIPFTAAAQVQRYGNKFATNIDGSNSAFGMRLYAP
                     GALGGSLFVYPTDSSASSPGPGVTVPLKRFSEGWADLEIPVGNLSDPFDPANLYQLTI
                     EVESGTAGPWLNPTVIYVDSIWSTNGLVRDTFDTTLGRMVASTLTTVAGSTMTWVASP
                     TP"
     gene            complement(32836..33378)
                     /locus_tag="VFQ61_32090"
     CDS             complement(32836..33378)
                     /locus_tag="VFQ61_32090"
                     /inference="COORDINATES: protein motif:HMM:NF018813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HET9959189.1"
                     /translation="MALTSTLYRLHLELSDVDRGVYQDLDLRIAQHPSEALDRVVARA
                     LAYAICLEDGIDFGKGLSDTEEPALWTHDLTGRLLHWIEVGLPSAERLHLASKRAERV
                     SVVCHKDLEMLMKELGRKKVHRAEHLRIFGLKPEFVAELAKTFERTTSWMVLLNDGEL
                     SVTVGARTFTTTVARPTLSS"
     gene            33673..35637
                     /locus_tag="VFQ61_32095"
     CDS             33673..35637
                     /locus_tag="VFQ61_32095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959190.1"
                     /translation="MNHSMGRAPRVHRWGNFAWGIALAIPACSGETTRAGESIQRVTQ
                     AAVTPTKITTLAQLRAMVVTGSYELGSDIDASSTASTPFVPIGTQASPFSGFFDGKTF
                     KINNLTIAGNRNYAGMFGYASGATIQNVKLNSVKLSNGSSYTYTGAIVGYMTATNLYD
                     SQVSGTVTGGSTTGGLVGYATGGSNLIGGSVTSLSVNGGSYVGGLVGYLTGSSYAING
                     TSSGGSVTGADSTGGMVGRASSGSLSYSNATALNVNGTTNTGGLVGSLTSGTIAYDSA
                     TGTVKGTDRTGGVAGAMTEGIAIFDSATNITVNGAADTGGLVGLLTRSTMDTGNVSGS
                     ITGGANTGGLIGRMIGTLARRSVLTLAYIDEKSAGNPSVVTGGTPVGMGVGSVQSYTD
                     LTKSYAIGKVTGSSTRIGGFMGEINATGIALANDEPRANVEEIFTQVEVTPAFDNTTS
                     NVFAGGLVGRLLGASIKNINVAGSVKGRNYVGGAIGYAVNDGANVTGSILRSILTRGE
                     VTNIAVQNRSGTIGGVSSKFMRCGRTYWDTTTDGGSAPPLPADEDPSCEVGLTSTQLK
                     APEKLYVDADHPNGNYDIFTYGMIIDREYQATWGALNCQLGSGSDGDFGFAFCYGVEP
                     SAEPPVWVMNSGTEYNTLVNIPRPERQPKN"
     gene            complement(35708..37168)
                     /locus_tag="VFQ61_32100"
     CDS             complement(35708..37168)
                     /locus_tag="VFQ61_32100"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9959191.1"
                     /translation="MARSSYGHPKLEQKQFSGSPPRTANQKSIMQPRVLIVDDKESVV
                     RMLRDALTPNCEVTTETDGLRAIALALRTEFDVVISDIRMPAIDGFELLTRLKEARPD
                     IEVILMTAFGTVQRAVAAMKAGAYDYLSKPFEPDEALLVVERAAERRRLRLEASNLRA
                     ALQASRTFENLCGRSPEMLRVLGLLAKASNSDVNVLITGERGTGKRVAARGIHRASAR
                     SDGSLSIVNCSANPAELSEGLFAQRDAVEVQSSGQMTSPSAAPGTVLLENVDELPMPL
                     QARLCLWLQQRPRAADPEPSPLRPRIVATSANDVKALLKSGALREDLLYQLNVFNIHL
                     PALRDRKDDIPLLAASFIEQHGKAYQSRVEGFTPEALSALVQYSWPGNVRELENAVER
                     ALAVVDEARIPLEALPDEVRGAGPGRSFSGLAQLTYREALEQARERACREYLVALMQE
                     FSGSVTRAAERAGIERESLHRLLKRYGVRSNEFRGD"
     gene            complement(37056..38558)
                     /locus_tag="VFQ61_32105"
     CDS             complement(37056..38558)
                     /locus_tag="VFQ61_32105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9959192.1"
                     /translation="MALDILPQESRPARSIAQRVALVFAMAIALLGTALGATVYSFRQ
                     LSRAERESAALDVARHTGHNVSILIREQYIHQAHTIIEADRSHLARYRAAATQARSAV
                     EQLLRLPLSDIERGEAAAISSLLTRADREFTEQVLPEVDRRAHARVRELHEQNEELGA
                     RVAALSESLNMMLETRAIRAGHAKEQLGRRAALVVIACFGLAIAVTGVAWMIIGQSLL
                     RRLSVLRTAAIELSGGELNARVPVQGSDEVADLAATFNDMAASLTNHQSRLIQAQRLA
                     VIGQVAAGVAHELNNPLGVMLGYIRLLQRPSPPPNALQVIEDELELCRRIVQGLLELA
                     RPPAGIRGPVDMDQLLRESVERLTDGEKLAGRTVNIAPNPSPLYVLGDAAAIRQVILN
                     LLQNAVEATTLTGTITIESYSSPGWVEVRVRDNGPAVTKATLDRAFEPFFTTKAKGTG
                     LGLAISQAIVHAHDGTIELRPSEVGTEAIFRLPSANSKPEVDHAASRSNR"
     gene            complement(38787..39773)
                     /locus_tag="VFQ61_32110"
     CDS             complement(38787..39773)
                     /locus_tag="VFQ61_32110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9959193.1"
                     /translation="MHGSFEFGQGLHIDPLDPSFQRPDPGTFTPRRRRAFASQSMARF
                     RESLLLPHTQSVRDAVLDDLSAFFGYSAEECRQRCLHWEKWSAEEWKDGDRSTDDGIA
                     SFYQSTKSWSFDLLWYAYLQAEGYAYPVSVAIAEGVPPAKDAPEHLDFGSGIGATGQL
                     FSRLGYRSSLADISSSLQEFAKFRLDRRGDVVTFIDLNKSALDARRYDVITAIDTLVH
                     VPDLPRVLEMLHLALKPGGLLFANFDTRPPSEANAWHLYADDLPLRWTLQRAGFEPLE
                     SLDLMSTKYQWVDPTSVTHMARGVRDLVLLKSPLRPAYRKTKTIVRQLLRRA"
     gene            complement(40358..40921)
                     /locus_tag="VFQ61_32115"
     CDS             complement(40358..40921)
                     /locus_tag="VFQ61_32115"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9959194.1"
                     /translation="MPKLWNDTIEAHRREVHEAILNKTALLVAKHGLRGVTMSQVAAE
                     TGIGRATLYKYFPDVEGILRAWHEQHINAHLEQLVATGTQAGNPAQRLAAVLEAYGMI
                     TREQQTTELGILLHQGEHVARAQERLRKFIKELIADGIASGDFRNDSSADELAWYCLH
                     ALTAARELASKAAVRRLVAVITSGLRA"
     gene            41003..41215
                     /locus_tag="VFQ61_32120"
     CDS             41003..41215
                     /locus_tag="VFQ61_32120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959195.1"
                     /translation="MKDSDDSGRSRHERVGRVEPPKPPPAPRWVKVLGLAALAFLLVF
                     LIVHALGGGLGRHATGGMHHRERSGP"
     gene            41215..41736
                     /locus_tag="VFQ61_32125"
     CDS             41215..41736
                     /locus_tag="VFQ61_32125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959196.1"
                     /translation="MARSLRSLGLTGHVAASVGWLGAVMCFDVLAFIGLYGSDPQLTR
                     SSLLLMRPIVEYLIVPLCLAATITGVVQSMITKWGLFEHYWVVVKLVLTIVATLALWL
                     HLSPIRDVSAAAKLAPALPSSLSATRLQVSVDATLATITLLVLTGLGVYKPRGVTPYG
                     WRKHNLRWAHSKS"
     gene            41964..43073
                     /locus_tag="VFQ61_32130"
     CDS             41964..43073
                     /locus_tag="VFQ61_32130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HET9959197.1"
                     /translation="MMSAPQTSNILVVDDKVENLRLLANILESLDYEVRPATSGRSAL
                     LAAERDPPDLVLLDVNMPDMNGYEVCVAFKARPRLKDIPVIFLTALNEVADKVKAFAV
                     GGVDFVSKPFHIDEVHARVKTHLDLRRAHLELEASYEKLKQLEQLRDDLVHMVVHDMR
                     SPLTVLGGHLACVQVEANRLSREATEDLRAAMLGVQALTRMANDLLDVSRLEEGKLPL
                     ELADHDLVEMAADVQSAMREFERGKRLELLASEPLHVNCDRNVVRRVLENLVSNAIKH
                     ASSTGAVRIELSRTPTGARLCVCDDGPGVPAQARQRIFEKFGTIAARRANGYHSAGLG
                     LAFCKLAIEAHGGRIGVEDGRAGGSRFWFELPREP"
     gene            complement(43096..45192)
                     /locus_tag="VFQ61_32135"
     CDS             complement(43096..45192)
                     /locus_tag="VFQ61_32135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9959198.1"
                     /translation="MRTLKGLWLRCSFWPTFADEERTQRARTFHGVACWTIVVIAAFL
                     LLLMLAQPETAGRRLRTLGFLLALVIGLLWLNGRGHVRLASWALVIGLGSIVTARAYT
                     SGGASAPALSLFVYICMAAGTLLGTSGGLVTGIGLAAIGYWLAQLQVNGLLPPSQLNF
                     TPLVGWAYSCMAIALSVLLHPQITLTLSRSLARANAEITARKLAEEERGRLLDELRHH
                     QEHLEQLVSLRTHEMQQAKEEAERASLAKSTFLATMSHEIRTPMNAMLGYAQLLRRDT
                     QLNEKQRAQVETILSSGEHLLTLLNDVLEMSKIEAGRAELAPEPFDLPSLLHGVHQMF
                     AALANAKGLSLEFVLAPDLPPQIEADPGKVRQVVINLLSNAVKFTTRGGVSLRATRER
                     SCDHRHTLKIIVEDTGCGIETADLERIFGTFEQAKAGASAGGTGLGLSIGRHLARLMN
                     GDVTATSRVGVGSSFTFVFEATAASDEALDKLRRGRIIGLVPGRPQKKILVIDDVPDN
                     VRVATTLLGTIGFETQSANSGEAGIAVHDDWKPDLVLMDLKMPGMGGLEAIRRLQEAG
                     TRAVLVAFTASGFEDLAERARQAGAHEVIFKPYRETQLLEMVAGLLGVELLYEGDSRA
                     PHTRDPPLRPLSLLLQKVPTALRQELREAAIQARARRIESVAERLSVLSAEAAIQVRE
                     LAREYRYEDLVRALDD"
BASE COUNT         8458 a        14024 c        14192 g         9030 t
ORIGIN      
        1 gtgatcaacg gcatcctgaa cgtggaggcg gtgcgtattg cgcaggccaa gtacggcaag
       61 cgtgcattga ccggcgaaga gacacgctgg ggcttcgagc acctcgacat caacgaagct
      121 cgcctgaagg aactcggcgc agagggactg ctgcagccca tcaagctgtc ctgcgaagac
      181 cacgagggcg gcggccgggt gcgcttccag caatgggatg gtgagaagtg gaagctgatc
      241 agcgactggg tcgcggcgga ccgccccttg ttgcgcccaa tcatcgaagc ttcggcggcc
      301 aagtatgccc aggagaaggg catcaagatt cgtgattgct ccgcagattc cgaaaagtag
      361 tccgaccctc ggctcaacta ctcgggaaga aatcccccaa tgacggactc gacgcctttg
      421 ctcgaactcg atcagctcga ggtcgcctac gacggtatca ttctggcgct acgcggggtg
      481 actctagcgg tggctccggg tagcgtggtc gcgctgctcg gcgccaatgg ggctggcaag
      541 actacgacgc tcaaggcggc ctcaggccta ttgagcgcgg agcgaggcga aatcttgcgt
      601 gggcgactgc gctatcgagg ccgagatagc gccgccacgc cggcccgagt actcgtgaaa
      661 caaggactcg tgcaggtgct cgaggggcgc cactgctttc cgcacttcag cgtcgaggag
      721 aatttgatcg cgggcgcctt ggtcaaacgc ccgaaccggg ccgagttggc gcgcaagctc
      781 gagcacgctt atcagcggtt tccacgcttg aaagagcgcc gccggtcact cgccgggctc
      841 acgtcgggcg gagagcagca aatgctcgcg atcgcccgcg ccctgatggc cgaaccgcaa
      901 ctcgtattgt tggatgagcc gtcgatgggg ctcgcgccgc tcatggtgca ggagatcttc
      961 gagatcgtga agtcgttgaa cgagctcgac ggcgtgagct tcctattggc tgagcagaat
     1021 gccacgctcg cgttgcggta ctcgcagcat gggtacgtac tcgagaatgg caaggtcacc
     1081 acgtctggtc ctagcgcgga gctcgccaag cgtgacgaca tccgcgaatc gtaccttgga
     1141 ttgaagaaca acggggcacg ccgacctcgc tcgctacgcc ccaccaatat cgtaacctga
     1201 agtgcgcctc ggcaatctgc cgtgaagcca ctgctctctt gagaaagaac gactgtgttt
     1261 gatcgaacga tgagatcttc gctcgcctta gtgtgtgttc tggaagcgct aacgattgac
     1321 ggacttgctt ctgcgcaaga cgcggccacg ccgcccaacg cggccgccgc cccgactccg
     1381 accgggacga ccacggagcc cgcaccgccc gcgccggtgg tgaccaatcc gaactcgccg
     1441 gctcctccgg gagcgggtgt gttccccaac agccctgcgc ccgacgtaaa cgcgccccgc
     1501 acgaacgacg ctgcgtcgtc taacgccgcg ggtgcgtcaa ccaagctcaa tgcgacatcg
     1561 gatgtggcgc cggcccggga agccgcgagc tcctggaccg agaagctcaa gctcggaggc
     1621 ggggctaccc tttggtatta tcaacccacc gtcgacggcc agaagaacaa cctcgacttc
     1681 ttcaacaccc gcctgacgat cgacgccgac ttcggagatg gcttcgggtt ttatctggag
     1741 ccccggattc gcgacaccaa gctgcgttcg ttttttgccg gtacggcatg gatcgagcaa
     1801 ggctacgctt cgtaccgctt cggcgaccac aaactcaaag tcggtaaggt gtactctcag
     1861 ctgggattgt tctgggacaa ttcattctgg ggaaacgtgc aggtatacgc gggcctcaaa
     1921 ctcgctccgg actatggggc ctcgctcgag ggcaagttcg aggtcgatcc ggcctggggc
     1981 gttggttatg ctgcacagtt cttcttgatt gacggatcca ccaacgtgtc tttgccgggt
     2041 cgggacacga tttcggtgcc ggactcacgc cgtcgcaatc aggtggtttt gcgcgcggaa
     2101 ccgtatctca actttgccaa agatggtgtg gccaaggtgg gcctctcaac tcagcacttc
     2161 gacgccgact caccggcaat cgccgacggc tccgtgtggc ggtacgcggc cgacctgaaa
     2221 gttgcgctgg gcggctttgg cgtgtggggc gagtatttga gacaacaggg gcagagtgtg
     2281 tcagcctacc cgatcgagga aatcccggcg accgataccg aaggcgcggt gcctgggcgc
     2341 gccgccaaga agattgacta ttacttggca ggggctgagt atacctatgg tcgtttcacc
     2401 gctcgctaca ccttcagcgc tgcgcgttac gcagggctga aggtgaaaga gtggatgcac
     2461 gtgcccgcgc tcggattcaa ggtcaatgac aatctgcagc tcggggccga gtacgttcac
     2521 tggacccgtt cgttctccgg aaaccacgac aagtacaatc gcagtctgaa cgtattggtg
     2581 ttcgtgaaat tctgagggcg ctatctgcaa ctaccgagga gcccgatgtc taaacaagta
     2641 gtgttgattt caggaagtcc gagccatcct tctcgttcgg ggacagtgct cgattggttt
     2701 gggcgtgcgc tgcgtgaagc gggttggcag gtggagacat tcacgttgga tgacttcgag
     2761 cctgcagctt tggtgcgtgc ccaggcggag gacccagcgg ttacgcgggt acgccgcgcg
     2821 gtcgactcgg cggcgggcgt ggtgttatct actcccgttt ataaggctac ttacgcgggg
     2881 gcactgaagt tgatcgtaga cctattcgat cccaaggctc tgcagggcaa agcacttttg
     2941 ggcattggca ccgcgcgcat tccagagcac ctgtccgagg tggaggtcag cttccagcgg
     3001 ctggcccagt tctttcaagg aagtgttcac gtgccgggac tcggccttct cgatgcggac
     3061 ttgggggcgt cacccaatta cgcgttgact cagacgggtc gtgtcgcatt cgaggcggcg
     3121 ttggcccgtt atctcgaggc gctgccgtga atacgccacc aagcgttgct aggacgaagc
     3181 cggcgtgggt ctggggggag acggcgcgcc gagctgacgc tcgacacgat cgattcatgg
     3241 cgcgcgccat cgggctcgct cagcgcggcc gtcccgcacc caatccgcac gtgggagcgg
     3301 tcgtggtgcg cgataatcgg gtggtgggaa tcgggcacca cgagctagcg ggcggccctc
     3361 acgcggaagt cgtggcgctc cgaaacgcga gttggctcgc gcggggcgcc actctgtatg
     3421 tcacgctcga gccttgcaat cactttgggc gtactccccc ttgtgtcgaa gcgatcctgc
     3481 aggcgggcat tgcgcgcgtc gtgattggat gttccgatcc caacccgcga gtcaagggtg
     3541 ggggagcgca gcgcctgcgg gcggcggggg tggtcgtcat agaaggcgtt cagcagcccg
     3601 aagccttgcg tctgatccag ccgtggctgg cgaccttgaa ctttgcctcg cggccgccct
     3661 attcggcgct cgcaagccac tcgactttgg atgcgccctc tgaagggggt taggtctcaa
     3721 tcgcgcgaat tcagattggc gcggagcgcc tgcgacgctc gtcccgggag caggtctctc
     3781 gcgtagacac gatacgtgac ctcttcttcg agcacgagac tcaggccgcc tacgccttgc
     3841 tcccgctgtg cgcggagcgc ctggacgtcg ctctcgatct cgacggtctg gcccgcgtct
     3901 tcgctgaacg cgctcgcggg agcgtcgggt tccagagcgc cctcgcccaa ttgagtttgg
     3961 agtgtagagg gccaagcttc gccctcgagg tcctcgacgc tgccgtccac gcgctgtgcg
     4021 agcactctca aagcgggggc gctgccctga cctgcgtccc agagctcggt catcacggaa
     4081 gtcacgctct cgaaagcggc tcggtattca gcggggccgt cacagctgca ggtgcagctc
     4141 agtcgggatt tgccgtcgta cagcgttcga gtgctggcat caggacaagc ctcgccgcgg
     4201 aactctgcat atgcggcgaa gcgcccataa agcagattct gtgaaagctt cttggcttgt
     4261 gcggaggaaa gcctgccgta ctgggttccg ctctcgaagt cgcgccctac ccaataattg
     4321 cactgcccgt ctacggtgaa aaactcatgc ccgtacggcg tggtgaatcc gtatgattcg
     4381 ggtaagggcc cgccccctgc attggtgtag ctcaatcgga tgctgctgct tccgtcacac
     4441 agctcggccg tgtccgaaag gggagccacc tgactttccc cggaacatgc cggctgcgcc
     4501 atcgccagca gcgcaaaagc gaaactacga atcgaccaat cgaaccgtcc agttgccatt
     4561 ccgaatctcg atcacgtggt gcagagccat caataccgag agcggcccgc gctcgcgagc
     4621 tcctcgcgtt cgcgttcggc gcgcgtctcg gccaaggcct cgtcgatcaa atcgcgcaaa
     4681 tcgtcttctg cgcgcggcgt cttcgcggag cgcggtgtaa ccgcgggagc ctctttcccg
     4741 agcagcgcct ccacatgttc gaggctatct cctcgctcca atgcctcgag cgcggcttgg
     4801 gcggagtcct tcgggcggct tggcttctga ccgaacaggc gagatagcca ggagcgggac
     4861 tccttgagct gaaatttgcc gctggcgact gcgttcgggt ggctcttcag cttctggatc
     4921 aactccggat tcgactgcac cagagtccag gcctcgtcca aagcggcctt ttgcgtcgtc
     4981 gcgagctcgg ccagcgccgc gcgtggcttc tgcccctcga agtcgaggaa acaagccagc
     5041 gccagtgcca gctctgggca cgacaacacg gtggccttac ctacgctcgg gcctccgcag
     5101 ctcttggaat acaagtagga ggcttccagg agcagtactc caaaaccgag ctgggtcgcc
     5161 gctagttccg cggcttcgac gcccgtttcc ttggcgttcg ggtcatcttt tccggcttcc
     5221 agcaagcgca cgtgcccgag catacgggcg atcgcggccg tgaaagcgat cgggtggctc
     5281 atggccgagg cattgacttc gatcaggtag ccgcgcgcgg tcttcgtcaa cgtgggcgcc
     5341 tcgaccttct tggtggacga ggttccgcag cctccgcttc cacaaccgcc cgtgccgcaa
     5401 ctggagccct ctgccgtttc gcccgacagc gcggtctcga tttcgatctc ctcgagcccg
     5461 gcgtagccct gcatccgagc cacgagcagc gatagcgaat ccgcgtcccc ggtgaacttg
     5521 tccggaaacc attccccgtt gggaagcacg agcgcctgct ctccgggctc gagctcgagt
     5581 tgcgccagca cctgggcata accgacgacg acgggacgca gctcggaagg ctcgggaaac
     5641 tccataccga cgagtttcgc agaaagcctc gggaggtgcc aggcagagcc cggctcggcc
     5701 cggaaagcct acaaaagcgc agagggcggg tctttcgatc ccgccctcgg gcgctgacgt
     5761 aaggtctcgg tctgtctcag gcgtcgcgga gccgggcctt cccggagcgc tgccgccgct
     5821 cagttcgcgg ccccgcccgc catggatctc cgatcactcg gcggcgaagg cgccgttgcc
     5881 cggggtcgtg cgcggagcct gaatgttcgt ctcgcgcgcg ccgtgcacca cgcccttgtc
     5941 gaggatgtgc tgggtgcggt cgaacccgtc caagttcttg ttgaagacca cctggtaaca
     6001 cgaggcgttg ccctgacccg caatgcacac cgaccaaccc aggaaaaagt gccaaatgcg
     6061 gtaccagcgc tcgccgtaaa cagccttgat ggactgctcg ttggacatcc agtagtcatg
     6121 ccaacggcga atcgtccaac cgtaatgttg gctcacgttc tcgaccgtga acgtctccca
     6181 gcccacctct tcggcaggct tcaacatgcc ggcgggcggc aagctcgcat ccgcgcccgg
     6241 gaagatgtac ttgttcatga acaagcccca aatcagggtt tcgccggaga gctcgcggcg
     6301 caggcccgtc cattggagta ggaacaaccc gtcgtccttc aggagctcgg acacccagct
     6361 gtagaacttc ttcaagttct tcacgccgac gtgctcgacc atctccaaac tgacgatctt
     6421 gtcgaacttc tgataaggaa tgtcgcggta gtcacagcac aggatacgag cgcgatcttc
     6481 gagaccccac tcgcggatac ggcgattgcc gaactcggtt tgattctcgg agatcgtcac
     6541 gccggtggcg tcaacgccgt agtacttggc ggcgtgacgg gccagggtcc cccagccgca
     6601 gccgatgtcg agcaagcgtt cgccgggctt gagacgcagc ttctggcaga ccaggtgcat
     6661 cttgttgtcc tgggcctctt ctacggtctg gttcggatcc gtgaagtagc cgctggtgta
     6721 caccatgcgc tcgcccaaga accagccgaa gaagtcgtta ccgcgatcgt agtgctcacg
     6781 gacaatgcgc ttgtcctggc tcttgctgtg aatcgtgacc tcgggcagga agttcgtcac
     6841 ggcccatttc gcgtgctcgg gagtgagcgt gtacttgacg aagcggttcc gctcgtcgag
     6901 cagctcatag agattgcctg gaatgtcgat ggctccgtcg aagtatgcct cgtacagcac
     6961 actcatcggg atcggggaat tcgcataacg tttggcgaga gtgcggtcat tgaaaacgac
     7021 gtagtcggtc aacttcttca tggcattcca atccggcctg tactcgtcgc aggctgctct
     7081 accctatgca cttgcgactt cggcgggtcc gtgaggcctt agatgtgcgg cgtcccgccg
     7141 tcaaaacaaa ccgcgctgga aatgcaattt cgcacctgct cggccggcgc acatgcagcc
     7201 acctgggtcc ccggcgcgaa gctcccggag cggagctcga cgctcccgga gctgattcat
     7261 cgacgatttg cccatgtttt gttcaggctg ccggtgccgc cgaacgtaac gtaagccact
     7321 cgtggacgat ggcgttgcct tgccgggcga ttcgggtgcg cagtttacgc ttggcgcggg
     7381 cctccagctg acgggcgcgc tcgcgagaaa cgcccagtgt gcgagcaatt tcggcgagcg
     7441 acagctcctc attttgatcc gccatgagac gccgctccgc gatgtacaat tctcgtgagt
     7501 cgagcacggt tagcgccgac cgcacggtag tttgcaggtt atcgccgagc tcgtgctcga
     7561 atagcgtctc ttcctggtcc tcttccgagg gcagtaggtc gagccagcgc acctgagcgt
     7621 cctctccgac gtgtgcgtcg agggagacgt cgcgcgcctc caggcgctgc agcatgcgct
     7681 gcatggtttc cggggaaacg cccatgcgct gcgccaacgc gcgttcggcc tcttctcctg
     7741 gcccgagcag gttagtgatg cgcgtgcgct cgcgccggag cttgaagaac acgcgcgaac
     7801 gcaaggggcc cgagccggcg cccaccaaac tccaggcatt gatgatgtga ttgaggacgt
     7861 aggctcgaat ccagtgcgcg gaataagtca cgaaacgcac gccacgctcg ggatcgaact
     7921 tgccgagtgc ttgaactagt ccgaagttac cttcggcaat cagctcttcg agcggcagcc
     7981 cgtaacgctg atacttcctc gcgatcgcca ccacatcccg gaggtgagcc cgagtgagcg
     8041 catcttgggc ttggcgatcg ccggtggctt taatgttgcg ggcgagtatt agctcttctt
     8101 cgcgggacag ccgtggagcc tcgtgcgcct ctctgagata gcgggcgagc tccggattca
     8161 tggcagggaa attcatcttg ttggccaatt cgtcagggtt cttgtttgaa aggaccgaat
     8221 cagtggacgg atcgaacgga gccgaatggg gcggggaacg gccagtgcga agagagtgcc
     8281 aacgcagccg ctcctccgtc cccccacggg cctcactcgt tcaacgtgtc gttgattttt
     8341 tggagcgctt cctcgagcgc aaggcggatg gcccgcatct tggaagggga caaccgtcca
     8401 cggtgcattc caagcttcac cgtgcgcatc agccgcccga agaacttcat caactccccg
     8461 aagtcatcgg tgtaggattc ccaggaatcc gcatcgaagc gcgaatagaa gtcggtgacc
     8521 gcctgccggt tggcctcgag gtcctgcttt cccgcgtcgg taatcgcata aaccttgcgc
     8581 ccatcctgct cgatgacacg ggcgtgcccg agttcttcga gcatctgcag agtcgggtaa
     8641 atcacccccg ggctgggccg ataagccgag cccgcacgct gctcgatgtg ctgaatgatt
     8701 tcgtagccat gccggggctg ctgctcgata gcctcgagca ccaagtagcg aacgcctccg
     8761 cgttcggcgc gagctggcgg tggttcgccg atcgcttcgg cccaccacgg acggcctccg
     8821 cgcggaggac cggcgaaccg accgccgcgt ccaccaaagc cccggctcat gtgccagccc
     8881 cattcgttgt cgcaaccatg tgagtgcatc ttcttttctt tctcttgaat gagaagccgc
     8941 tgaaaacagc cgcgttctca gttttcgata tgtctttaga cattgctata gatatatcgg
     9001 agagaccgtc aatcggccat ctcgcagtcc atgagaaaat tggtcgactg gccgaagttc
     9061 gggtgtgcct ccggcgcaac ccgccaagcg ttcgcttgcg tgagtccgcc atccgtgaga
     9121 atttgcgcgc cccaaacagc gactagcgcc cgtagctcag ttggatagag cggtggattt
     9181 ctaatccact ggtcgggggt tcgaatccct ccgggcgcgc tgaacccttc gggttcatgc
     9241 gcccggagtg cttcaggccg cttcggaccg acggtgtcgg tcctaacggg agcgagtccg
     9301 tccgggcgcg ctgaccccgg gtttcatgcg tccggagtgt ttcgcgctta cctggggtta
     9361 ctgcgggagc gtttcgggct gattgggact aactgtgtcg gttcttggtg cgaatccgtt
     9421 agccggattc ctattccgtg aggcgataaa gtgtttgggc cgggcatatg tccgttaccc
     9481 tggtcttttc aggttcgtgc gccctgtgga gcttgttgtg tgccctgagg gtgggtgttg
     9541 cgaacctcga ttgggcgccg gcgagtagcc ggtcccgagc acttggcgcg cgacggtcgt
     9601 ccgaggaagg acaactgtcc ggggacactg cgtggcgaac ggaccacccg aattggtagg
     9661 tcctgagttc gtatgcggcc atggagagga agcgtatacg ccgccgcgga cagcttctgt
     9721 ccgcaatcat cagcctcgag tcgctacgtg ctccacgagt tcgcgcacga gcgggttggg
     9781 gaagcggcgg ctcggtgaaa tcgcgtagaa ggtttcgttg aagcgcggca gccgccgttt
     9841 ttcgatcaac gagttacgct ctagttcgtc tttgacgacg attcgcggta ccaaagtcag
     9901 cccttctgtt tcttgcgcga gaagtcggag catggccata tcatccacct ccgcggccac
     9961 gagcgggcga atcccggctt catccatggc ggcgtcgaag gcggcccgta ggttgctgcc
    10021 agggcccggc aatgtcagtg caaccgcggc gcagtcctcc gggaagcgga actcgcgacg
    10081 tttccaatgc accccgccaa ctagactcac gggctgctcg gcgagtaaac ggctgtgaaa
    10141 tgaaaccctc gtaccgccgg gtgcaggctg attggagagc acgatgtcga tctcgtgctc
    10201 ctgcatgagc gtgagcaagt ccgacagtga gccggagcga accaccagcc caatatcgct
    10261 gcgggccagc aagggctcga acgctccaat caggaagttg cgagagaggg ttgccacggc
    10321 cccaacgcgc accacccgac gacctctcgt gaggccgtgc gtcatcacgt cctgtagctc
    10381 atcacccgca cggaatatcg tttcggcgta gtcaaaagca atgcgcccgg cctcggtgag
    10441 cgtcggtcca gacggagcac gctcgatcag ctgatggccg agcagggtct cgagttgttt
    10501 gagctgggca ctcagcgcgg gcggagaaac gcgcaattgc tctgcggctt gccgcatgct
    10561 cttcgccttg gcgatcgcgt agaagtaccg gaggtgatgg tagttgagct gaccagccat
    10621 gaacaaaaac ttaccacgct ggtaatgact atgtaatcgt cattaacttc gagttgcttt
    10681 acgttccgcg cccatgagct ggtcgaaagc attcttgctg ttcattccgc tcctgcccgc
    10741 tttggcggga gcgctgcccc tgcgccgacc gaaaacggcc ggtgtggtca cgagcacggc
    10801 gtgcgggttg gccgccagtg cggccgcctt tgcttgcgtc caggcgtatt tctcgccgtc
    10861 tctgacgacc ctgcgttggt cgctcacgtc gacctctcca atctcactgg ggctgcagct
    10921 ggatcgcttg tcggctacga tggcgctcct catcacgctc gtaggctgct gcgtgcttcg
    10981 gttcgcggca cgctatctgg agggagacgc ggaacaggtc acgttcctgc gccgcgttag
    11041 cctgaccatc gccgcggtcg ccggctttgc cttggctggt gacctcctcg tcttgctcgg
    11101 agcttggatt ctggcgagcc tcgtgttgca tcgcctacta acgcattacc cgcagcgccc
    11161 cgccggagtt agagccgcca aagccaagtt tctcgtgagt cgattggcgg aaggagccgt
    11221 gctctgcgcg agcgttctat tgtaccggag cttcggcacg ctcgaattcg agacgctctt
    11281 tcttcgcctc gagcaagcct cgcccgagct gctgcaatgg ccggcgctct tgctggtgtg
    11341 cgccgcgttg ctcaagtcgg cccagttccc gttccacagc tggctgcccg acaccatgga
    11401 gacaccgacg cccgtctcgg cactgatgca cgccgggatc atcaatgcgg gtgggttttt
    11461 gatgattagg atgagtcccc tcttgggcca ggtgccgatc gccactgggg tgctcgcgct
    11521 ggcgggagca acgaccgccg cctttggcgc cctcgtcatg ctcacgcaac cgagcgtcaa
    11581 gcgcgccctt gcgtattcga cgatagcgca gatggggttc atgttgctcg agtgcggcct
    11641 cggtttgttc ggcctcgcgt tgctgcacat catggcccac tcggtataca aggcttccgc
    11701 attcctgcgc gctgggagtg cggtgaacgc ggctccgcgc tcgctcgttc ccctagattc
    11761 gcgtgccatt ctcgcctcgt tgaccgttgc gaccggattg gtgctaggcg cgagtcgggt
    11821 tggacactcg gcgcacagcc tgggcgcggt cgtgatggcc gtgattctcg cgctatcgat
    11881 agcgtacggg ctcactcggt actgggctgt gtccgaacgg cgcttcgctt ggatgggctt
    11941 gagctacgcg gccgcgttca cgggcgcggc aaccctggtt catagtctcg gggcgactct
    12001 tgtcccggcg cggtccgaag tctacgtggc gccctggctg gccctgcttc tctgcacgct
    12061 ctttatcggc ctgtttgcaa ttcaggtttc gctctggcga atggcgcgac atgcactcgg
    12121 tcgagccctc tacgtgcacg ccctgaacgg gttctacgtg agcgcattgg tgaatcgagc
    12181 tctggagcgc tggtggccgc cgcgacgata cgagctcgtt cgttcaatcg agcccagttt
    12241 ggcgcaaagg atgactcaat catgaacacg gaaatgcgag aaccgtcgca atgcggcgaa
    12301 gacgtgaacg cggctcacga agcagcagtg cgagaggcac tggccagaat tgctccgctg
    12361 tttccatcgc gcagtttcgt cgcggtgaat cccttcctgg gtttcacgaa tttacccttc
    12421 gaagaggcag cctggcgggt gatgacatta cgtggtcggg ccccgctgct cgatgcaaat
    12481 gaatatcgaa agttgtttgc gaagggtgag attacaatag aggatctcga ttgggtgacg
    12541 gacgcgagtt ggtccgtcgc gcagctgctg accgcgctcg agtcgccggt ggaccacgct
    12601 attgacgtgc cctggaccgc ggtcgacgcg ctcgaaagcg aacgagcaga agagcgctgg
    12661 cgtgacttcg tggtcgagga gatctcgaag tggtgcgccg tctattacga ccagaaccag
    12721 acgacttgga ccctgccgtg ggcgcgggcc gggctctaca gcgtgttccg cgaggcggcg
    12781 cgcttcgatc gggccccgga cacccacggg ttgcacacgc ttcgggattt ggtcgcaacg
    12841 ctgccgcccg acgcggaggc ggcgatcgca gtaatcttga gagaactggc tccaccctcg
    12901 ggacgggagg cggacttcgt ttacgggcaa ctggccctcg tgagcggatg ggcggggtat
    12961 tgccagtact tggtccacga gcatcgtttg aaaggcgatg atagtccggc gttgcgtgag
    13021 ctgctcgcct ttcgcttagc gtacgatctg gcactcttgc ggatgcgacc aaatctgcgg
    13081 tcctcgtttg gcgttcgccg ccgcagcgtc acgaccgcgc ccctcgcggc gctttgccgc
    13141 tggcaattgg catacgaggt ggcctggcaa cgtgctgtct tgcggaagct tacgcaggcg
    13201 cacgaccctc tctctcccac cgagccgaag cgcccgcgag cgcaagccgt gttttgcatc
    13261 gatgtgcgct ccgaaccgtt gcggcgacac ctggaaggcg tcagtgagga cgtgcggacg
    13321 ctcggcttcg cgggcttctt cgggtttccg ctgagtcacg agcgccccga cggtagtggc
    13381 gccgaagcgc gttgcccggc attgctggct ccaacggcgg agagtgctga gtgcggagtg
    13441 cgacgccacg accgggccgt cagagcacgt gctcgcgcat cgacctcgaa ggcaattcag
    13501 aacgcggccg cctcctgctt ctcgttcgtc gaggccgcgg gcctcgcctt cttggcgcag
    13561 ctgtggtcgc agacggacac tagcgggccc gcgtggctgg gcacacccaa accggcgttg
    13621 ctcgacttcg agcacagcga cctcgacgcc cttgcggtgc gcgccgaagc caccctgcgc
    13681 aatatgagcc tgacgcgaaa cttcggaagg ttattgctcc tatgcgggca tggtagtcac
    13741 agcgccaata acccgttcgc atctgggctc gattgcggcg cgtgcggggg ccatgctggt
    13801 gacgtgaacg cccgaatcgc ggcgcaagtg ctaaatcacc ctggtgtgcg gcagcggctg
    13861 gctggcaggg ggatcgagct gcccgaagat acttgggtgc tcgcaggtct gcacaacacc
    13921 atgacggacg aggtcactct gttcgatttg gaccttacgc cagaaagcca ccgcgccgac
    13981 gtggacgcgc tgctacgctc cctcgaactc gcgggcgcag ccacacggcg cgaacgcgcg
    14041 gtgaaactcg gacacgccac cgatgaggac ctactctacg cactgcgaaa acgagcgcaa
    14101 gacatcgcgc agacgcgacc ggaatggggc ctcgccggca acgccgccat aatcgttgca
    14161 ccgcggcaga ggacgtgcgg gctagatttc gagggcagaa cattcttgca cgaataccgg
    14221 cacgaggatg accaagacgg ccgcatgctg caactcattc tctcggctcc ggtcgtggtc
    14281 gcgagttgga tcaatctcca gtactacggc tcacgagtcg atcccgtcct ctacggcgcc
    14341 ggtaacaagg tcctgcacaa cgtagtgggc ggggtcggag ttttcgaagg taacgggggt
    14401 gatctgcgcg tcggtctgcc cctgcaatcg attcacgacg gcgaaaggtt catccacgag
    14461 ccgaggcgac tcagcgtgtt tgtcgaagcg cctcgggaaa aggtctggcg cgcgatggct
    14521 tcgctgcccg atgtacgaaa gctgttcgac ggccgatgga ttcacctgct caccatcgag
    14581 cacggtgaag tgtatgcgta cgaaccaagc gggtttcgga agctggagtc gactggcact
    14641 cggcagagcg tagccgcatg aacaggtgcc cggcgttgtt ccagctcatg gtgccacccg
    14701 accccagcct gcccgacacg ggcgtgcttc tgcttccaag cacgtgaaga cttacgaata
    14761 gccacccgac cccccaggtc gtacaagaaa gctcgaccca attgctgcga attgtccaaa
    14821 agctccccgc ccacgcgtca tagggtatcg atccacagga gagaccatgc ccaagtactt
    14881 gtgtttgcag cgcagcctgc cgggaaaaga gtcgagctcg gataagccct caccagcgca
    14941 aatgcaggag atgtatgaaa agttcaacgc ctggcgggaa aagtttcaga acaatctggt
    15001 cgacctcggc ggccgtctag gggagggacg gctcgtgtcc cctgaaccgg ctccggacgg
    15061 gccgttcgcc gaggtgaagg aaatcgtcgg cggttacatg attgtgtccg cttcgaattt
    15121 cgaggaagcg attgcgatcg cgcgtcagtg cccggggctc gttcgcgcag gctccggcgt
    15181 cgaggtgctg gaaatccgtt ctcctggatg acttccgcgc tcggcgagca cttctttcga
    15241 cacgagttcg ggcgactcgt ggctatgctg agtcgccgcg ctggggtgca gcacctggag
    15301 ctcgtggagg acgccgtaca ggtcgcgctg ctggctgcgg tcgagtcttg gacgcgaacg
    15361 ttcgtaccag cgaatccgtc ggcgtggctc taccgggtgg ctcacaacca cctattgggt
    15421 gagctgcgcc ggcggacgcg gcgcgatgag ctaaccggat cgctggtgga gcattcttcg
    15481 tcatcgattg aagagcccgc tgttcttctg tccggcgacg tcccagaagc ccaattgcga
    15541 atgctgttcg tgtgctgtga cgacagcata gccatcgagt cccagctcat gctcgcactc
    15601 agaactctct gcgggttcga tatttccgag atcgcccagc gtctcttcac gagtgagacc
    15661 aacgtctaca agcgactcgg ccgggctcgc tcacgcctgc gcgagacgcg actcgtgacc
    15721 gatgaaatga gcaccgaaca gctcgcatct cggctgccgg cggttcacgc gatcctctac
    15781 actctattca cggaggggta tctgtcttca catgcagagt acgcgattcg ccgagagcta
    15841 tgcgacgagg cgcggcgttt gaccgccttg cttgcggcgc atccggtggc agcaacccct
    15901 gaaaccttcg ctctactcgc gctcatgcac ctgcatatcg cccgaatgag cgcacgtcag
    15961 gatgggagcg gcgggctcgt gctgctcgaa gagcaagatc gctcgcagtt cgatcgagag
    16021 gagatcgtgc agggtctgtc ttggctcgcg cgatcagcaa ccggagatgt cttctcgcgt
    16081 tatcacgcag aggcgggcat cgctgccgaa cactgcctgg cgccgtcgtt ttccgaaaca
    16141 cgctgggatc gcatcgtaga gtgctacgaa cttctcgatc ggctagcccc gtcggccatc
    16201 catacgctca atcgcgcgct tgccgtggct gagtgcacga atgcagcggc aggcttggcc
    16261 gtgatcgaag gaatcgaacc gccatcgtgg ctcactgggt cctacctctg gtctgccgtc
    16321 ttggcagatc tgaaccggcg cggtggccgc aacgagctcg ccaagagcta tcgcgacacg
    16381 gctatcgagc tggctccctc cccggcgatc gcggcgtgtc ttcgtcgtcg tctcgcctga
    16441 gaccgcctcg atccgcgact agactcgaac aatcgaagaa atcgaagggc gaaccgattc
    16501 cttcgagctc gggggacggt ctacacgggc atgagcattc cctcgcacat gttcgctctg
    16561 cagcagacgt ctctaaatgg gcctcggcat atgaaattga tcagcgacgc tccggtgcca
    16621 tcgcccggcc gcggtgaggt tttgatccga gtcgcggcgg ccggcttgaa ctttgctgat
    16681 atctcgaggg cctatggcac attccggaat ggtccaaagg ccccgtactt ggctggtttc
    16741 gagtcttcgg gtgaggtcgt cgcgttgggc gagggggtga cgagtccgca agtgggaact
    16801 cgcgttgtcg gcgtcggtta cggcgctttc gcggagtacg ccgtgctgta cgcggcggcg
    16861 gcagtgccgg tcccggtgag ttggaccgcg ccgcaggcgc tagggttggt cgtgaactgg
    16921 ccgaccgcgt tggctgcgct caggccgctg ggtcgcatcg cggcgggcga aacggtcctg
    16981 attcacgcgg cggcgggtgc aacggggcag gccgccgtaa cgcttgccaa acacttcggc
    17041 gcgagagtca tcgccactgc ctcacccagc aagcacgaga cggtgcgagc attgggggct
    17101 gagcacattc tggattcacg cgccgaggat ctcccgaccc aggtgaagaa tctgactagt
    17161 ggcgctggcg ccgatctggt gctcgagtca acggggggct ccaccttcaa ggctagtctg
    17221 agtgccacgc ggcgagtaac ggggcgagtc gttgtttatg gactggccgg tggcgaggcc
    17281 gcgctcagca actgggaatt ggtgtacgaa caacaggtcc aggtgatcgg attgaacatc
    17341 ggcgttctaa gtcaggccgc cccgcagatt ttcggcacag tcgtaggcga gttgttggag
    17401 ctcatcgcga ctggtgtggt gacgcccacc agccctacct cctacgcctt ggcggacggc
    17461 tcgaaggcga tcgccgcgct cgagagtcgc gccacgatcg gcaaactggc attgctacct
    17521 ggatgagtaa cggcttctta ccgaagttcc ccgtcaccgc ttaaactagc gacatgacga
    17581 agcgctcgtt agagacggaa gctcaagacc gcttcgcggc cgaaatgggg cgataccgcc
    17641 gcgagctgct ggctcactgc tatcgaatgc tcggttcctc ccgggaagcg gaggattcag
    17701 tgcaggaaac gtaccttcgg gcctggcgag cctatggaag cttcgagcgc aatgcatccg
    17761 cgcggtcttg gctctataag atagccacca atgtctgcct gaccgccttg ggctcgcgcc
    17821 ggatccgggt tctaccgtcc ggtctcgccg gtccctacga cggtcccgag cgcctggcgg
    17881 acatcgtacc gccgggcgaa gtctcgtggc tagaaccggc gccagacgct tggcttgacg
    17941 ccactactga cgagcctgac atgctcatcg aggcgcgtga atccttgagg cttgcactca
    18001 tcgcgagcct ccaatacctc cccgcgcgtc agcgcgcgac cttcctcttg cgtgagatcc
    18061 tggcattctc cgctgaagaa gcagccgaaa ttctcggcac cacgaccacg gctatcaaga
    18121 gtggtctgca gcgcgcacgc gccaccctcg accagaggat gcccgagcgt gaggccttgc
    18181 tcgaacctac cgactcgcgc gccatcgagc tgctcgccgg ctacattgcc gccttcgagc
    18241 gctccgatgc gagtctgttg ctgcaagtat tgcgggccga cgccgcgctc gaggcgacac
    18301 cattccgtga ttgggtgaca gggcgggtga agtgcattca gctactccaa aactacgtcc
    18361 ttggcacccc tggtgactgg cgcatgctgg cgacgagagc caacggccag cccgccgccg
    18421 ccttgtatcg acgggacgag aacggtgcgt tgcgatctta cggagtcgtg gtgctcgccc
    18481 ccacccccgc cggggtctcg cgtgtgatcg cgttccacga tccagcgtta aatcggttgt
    18541 ttggattcca agacacattc gagggttgaa ccaactgaac gatgttggca ctgccgggtc
    18601 ggaggccgtt actcaggggt gggtggcctg caactgctcg gtgcgccgca gcaggagaac
    18661 cgtgagcggt cccacgaata gccactgtac gaaggtgtac ccggactcga ttaatagata
    18721 gtccggcacc gagctcatga cattcttcgc cgcgacggcg atggtggtga aactccacga
    18781 cagcagggcc ccgaatgccg cataaccaag cgtgcgtgac ttgagatccg gccagcgagc
    18841 gaacacgcgc gcgtaaaccc acgcgaacag cagcgcctga atggacatgg acaaaaatcc
    18901 gaacgggata atcacatctt ttcggtagat ctcgagtgcg tcgtagtagc ccttgaatgc
    18961 gacaaggtgc cacacgaaac cgagcgcaaa tgtgggaacg agatagcaaa gcgcgccgat
    19021 agcgatacgt ttggtcatga cgattgcctc ctagaagcga gtaaccgctg cagttgatcg
    19081 accgcccccc gccagccctc cgcgtggcgc gcgttggcgt cagcgcgctc gaatcgctca
    19141 tgaagaatga ctaagtcgct gccctcagcg ttcggtgcga tcgatatcgt cacccgagat
    19201 tcgatgccag cgtggatatc cggtggctcg atcagccacg agaatactag ccgacaaggc
    19261 gcctcgatct cgcgatagac gccccccacc tccatccgct gccccccagg tgtgtcgtaa
    19321 acgaagcgat atcgtccacc ttctcggaaa tccaattcga gcacgctgag cttgacctcc
    19381 gggtctggcg tgagccattg ccctacgacc tcggcgctcg caaatgcggc aaagacccgc
    19441 tccggcgtag ccgccaagtg gcgtcgcacc tctgctcgtt cgctcggctt catggcgtat
    19501 ctcggccgag aagcgcctcc agctgatcga ttgcgccatg ccagaagcgc tgatgtcgtt
    19561 cgatccatgc tcccgcatcc tgcagaggct tttgacgaag gcgaaagcgg tgcacgcgac
    19621 cctcgatgct gcgctcgatt aacttcgcct gctcgagcac cttgaggtgg cgcgagatcg
    19681 tgggttgcgc cgaattgaac tgcttgccga gctccccggc actccgcgcg acgcccccgg
    19741 ccagcgcgtg gatcatcgaa cgtcgagtct ggtccgaaag tgcatgaaac gtgcggtcga
    19801 gagccgctgc ggattgcata gctgaacagc tatatttttg gctttcgctc gtcaaggccc
    19861 gcgtgacctg cagcgtacag tttgaccacc gcggtcaagt tttcgtccga gaggccactc
    19921 tccaggccgc gcgcgaagac atcgcgggcg gcactggtca tgggcatgtg cagccgcgtc
    19981 gccgtgcctt cggcgactgc gtagccgagg tctttcgtgg caagcgagat cggaaacatc
    20041 ggagtgaagc ccccggccag catcccggtc atggcgcctt tcgcggcggg gctcatcacc
    20101 gggagagcct cgagagttct cgccaatgtc gggagatcga gtcggccgcg ccgggccagg
    20161 gcgatgatct cggctacggc ggccacctgg gtcgtgaaca gggcgttcgc gagcagcttg
    20221 ctgagcgctc ccgcagggga cgcgcccaga tggtgaatcg cgcttcccat ccgaagcaac
    20281 gtaggtctca cggtgtcgag cgcggatgcg tcgccgcccg cgaggaagat gaggttcccc
    20341 gcttcggctt gggggcgaga gccgaccacg ggcgcgtcga tgaacgcgac tcccgcgtgc
    20401 cgcgcatgcc gggctaactc cgccacccag gtcggagtca gcgtgctgca ttcgacggcg
    20461 accgcgccgc gccgcatgcc tcgcaatgcg cccgaatcca gccatatgcc gcgtgacgcg
    20521 gggtcatcgg tgaccatggc gattgccata tccgctcccg cgacggcggc ttccggtgtg
    20581 ggtaccagat gagaagccag ggatgtgaat tccttgggca cgccggagcg gctccatact
    20641 tgcaggtcga tatctccgct taccaagcga gtggccatgc gcgcacccat tgccccgagt
    20701 cctagaaaag cgatcttggt tcgatgcttg gtagtcattt ccgcttggat ctgactcatt
    20761 ccaaagcggg agagaacatc gttctattgc aatcagttat tccggtttgg catagtctac
    20821 cccaatggat cgtctcgatg cattgcgcct gttttctcgt gtcgcggagc gagggagctt
    20881 ttccgcggcc gcgcgtgaac tgaaggtcaa gcagtcgacc gcgagtaagt gggtggcgga
    20941 gctcgagtct acgcttggca gcaccctcgt ggaacggacg acgcgctcaa ttcgcattac
    21001 ggagagcggt caacgtttgc tggtgcgggc acgggaggtg ctcgcggcct acgacgagct
    21061 cagcgccgac ttcgagaacc gcgcgcctac gccgcgcggt tcgattcgcg tgagcctccc
    21121 cgtcgtgttt ggcagactct acgtgctgcc cgctatcgcc gactttctga agtgctaccc
    21181 gcaagtggac gcggagatcg tgatgagcga ccgttacgtc aacttggtgg aggaaggctt
    21241 cgacctcgcg ctgcgcgtgg gcgttccaac ggataccagc gcgcgtggtc ggaagattgc
    21301 ggagagtcgg cgcgtcctcg tcgccgctcc cgcgtatttg agggcccatg gcacgccacg
    21361 gacacccgaa gatctgcgac ggcatcagtg tctggtccat ggagacgcga acgttcccgg
    21421 tacctggcgt tttgggagag cgggcgaatc tggtgttccg atttcagtga aaggtcgggt
    21481 cgcggcgaac aacagcgagg cggtggcatt gatggcacgt cggggactag ggctggcgct
    21541 ccttgccgac tggatggtgg ccgaagacat ccgacgtggc cgcctcgtgc ccctcctgcc
    21601 cgagtacgct ctgccaccag cgcccgttta tgctttgagc ccgcctggtc gattctcgag
    21661 cacaacggtg ggcgctttga ttgaccatat cgcccaagtc gttcccgcgc gtatcagtcg
    21721 cgcatagacc gtctgctcga ggtcgcgacc cgtactcctc gtcaaggccg gcccgagtga
    21781 taggctgcac ccgcaatgga tgtcctcgca gatgtgctct cgattacgcg tctcggggcc
    21841 accgtagtag cgcaggcgga gctggtgccc ccctgggggc tcgaaatcga tcccatcgcc
    21901 gagacccatg ttcacgtcgt cctgcggggg agctgctggc ttcgcactac gggagatcgt
    21961 cgccccattc gtctcggatc cggggatgtc gtgctgattc gggccggtgt cggacattcg
    22021 atctgcgacg atccgaagac caagccagct ccacatggag acgtgctcgc ggcgatgccg
    22081 ctacgtctgg cggcacttcc cgcatcgcgc gccaaggaga cgacgctcgt gctgtgcgcc
    22141 aagtatcttt tccagggggt aggtgcgccg ggcggtgcac atccgctcac gtcgctcttg
    22201 ccccctctga ttcacctgcc tgcttacgag gcggagcgag acaaacagct gcagctcttg
    22261 ctgcaattgc tcggtcatga agcaagggcc gcgggggttg gaaccgagtt ggtggtgcct
    22321 cggctggtcg atagtctgct cgttttcgtc gtgcgcgcct ggctggcagg gcaacctctc
    22381 gccgccggtg gatggttcgc ggccctccgt gacccgcaca tctcggcggc gctcggcctc
    22441 atgcacggac ggcccgagat cgcctggtcc ctggaacagc ttgcgcgaga ggtcgggctg
    22501 tctcgagcaa cctttgcgcg ccggttcctc gagctggtcg gcgaaacccc ggtcgcgtac
    22561 ttgacgcgat ggcggatgtg tctggccacc aagctactca gggagacgtc gctctctctc
    22621 gaggaaattg cgccgcgcgt gggttatcag acggcggcgg cgttctcccg tgcctttcgc
    22681 agaagccttc gcactgcgcc cggagcgtat cgcgcggagg cgcgagccca gggccagctc
    22741 gctcatctcg ctcccgcttt gtcgtgatcc gggggcgtca attgagacta acgggaaaga
    22801 actagtccct ggcgggcatg tcgcgggtca accgtaacgg ctaggttgaa cccagatttc
    22861 gcgcgaaatt cgagttcagc gaccctggcc gcatcgcgct ggaatgtgtt ccgtcgcggc
    22921 ggcccacagc aatgcaggta atgcgcaatg ttcatcgtac ttctcagatt ctctgcgaac
    22981 atcgaccagg cgggcgatct gatgatggcc cataacgagt ggattgaacg aggtctcgaa
    23041 gaaggaatat tcttgctcgt tggcagcctt cagcccaaga agggcggcgc tattctggct
    23101 cacggcacga cgcggtccgc tctagaaact cgggtcctgc aggacccttt cgtggcgcat
    23161 gacgtggtaa gtgccgagtt gctggagatc tctccttcca aaaccgatcc gcggctcgcg
    23221 ttcgtgcttg gttgaagcgc agcgcggtct gtgcgcattc gctccgaatc gaagctgcgc
    23281 tcactgtcat gcctttgtcc cggcgagctc caagagctcg tcgacggacc attggtcgtt
    23341 ggcgtgcgcg ccataccggg cggcgacgac gacgttgctc cgatcgataa gaaggtctgc
    23401 gggaagcccg gtctggtccc atccacccac gaacggattc gagggtgccg tgaacaggcc
    23461 ctttagcgct gcgcctacga cccgcgggtg tgcgaccgct gccgccgagc gctcgactcc
    23521 gaagtagcgg taccagcgcc gttctgcgtc cgcgacgcag gggaagggga gttggccctg
    23581 atacggcatc atctgctccg atcgggagtg gaagaacgcc accgtgagta cacccgcatt
    23641 gctgatgcgc ccgtgagccc gggcaaagct ctggaggtgt aagttacaaa cgggacaacc
    23701 cgcgaagcgc cgaaattgca ggtgaatgag ccgaatactt gcgttggggt ttggaactcc
    23761 gacggtctca ccgtggaggc tcgagaattg gagcgaggga agccgctcac cgacctggcg
    23821 gcgtttcgag gtgtccatcg cgaacatttg gccatgactc gagcattggt cgaatcgatg
    23881 tggaccatga ccaccatggc cccaatcgat ggtaaagggt cttcatgaat gttgatcctg
    23941 gcgcgctcgt ggctttgaac gtgctgctcg aagaacgcag tgtgacccgg gccgcacgtc
    24001 gattgggtat cacacagtca tccatgagcc atcgtttggc tcggctgcgc gaggcgctcg
    24061 gggatgggct gttcgttagg gtgggtgcga ccttgacgcc caccccgcgg gcgctcgcga
    24121 tcgcggaacc attggcggac gctctgcgag cgctcaacgc ggcggtggcg attccgctcg
    24181 cgttcgatcc cggacgcgcc aacggtagag cgtcaatcgt gatgccggac ttgcttgctc
    24241 ccttcgcccc ggatttggtg gcgcgaatat ctgcgcaggc acccggtctc gccctgcgct
    24301 tcgcgagcgt cagtggtgag ctctcgacgg cgctggcagg gccagagctc agcctggcat
    24361 tggtccctgc aagattcgca gccgcggata tgcagtcgcg tgcagtgggg gagttacgtt
    24421 ttggcgtcgc cggccgccgc ggccatcccg ccctcgcccg gcgcttgagc ttggagcgtt
    24481 ggctcgccga gcgccacgtt gtggtgagcg tcggcctcga tcgcagcaac gttatagatg
    24541 acgaactgaa tcgtcggggc ttggaacggc gggtgggtct ggtcgtccca tccttcctcg
    24601 cgggcttatt cagcttggcc caatccaact tgttgatgaa tgtgccaatg cccctcgtag
    24661 acgaagccgc caaacaactc ggcctctgcg ttcgcgatgc ccccctacca ttgccgcgtt
    24721 tacacttctc catggtctgg cacccacgct ttcagcacga tgccgcgcac ctctggctcc
    24781 ggcagcaagt gctggagctg gttcaacacg ccttccggaa acagccacgc gctgactcca
    24841 atactaaatc tcgctctcga gtcgagcgaa cgacgtctcg ctccaacggg gggacgtgac
    24901 cgaaaaccca tcgcagactg gttcgcaagc cgaagcgtga tagccttgcg gtcaaggagt
    24961 cgtcgaatgc gtattgccct cgtgtctgat ctccacggta acgctctcgc cgtcgaacag
    25021 gtgctgaatc ggattcgatt cgacggcgct gatcagctga tctgtttggg cgacgtcgcg
    25081 acgctcggcc ccgaacccga acgtgtcatg gagctactcc acgagagtgg cgccacctgc
    25141 atcctcggga atcacgacga gttcatgctt cggccagaga cggtcgcgga atacacgacc
    25201 atttctgttc tgattgatgc gatcgattgg tgccgagcga atttgagcgc cgcttcgctc
    25261 gagttcatgc gaagttttcg tgagcgctat gaggtgccgc tgaatgcgca agaggtgctg
    25321 ttggcctttc acggtacgcc ggcctccaac actacggatc tgctgtcgac cagcgacccc
    25381 acgccggtgg aagagtggat tggagcggtt ccgggcagcg tgctggtggg tggtcacacg
    25441 cacctccaga tgcttcgtca acaccgcggc agattgctgg tgaatccggg cagtgtcgga
    25501 atgccatttc gcgaatacgt cggtggcggc ccgcccgagg tgcttccgtt cgcggagtac
    25561 gccgtgatcg actccgactc acattcagtg gaagttcgcc tgcagcgcgt gaacctggac
    25621 gttaccttgc tccgaggctc cgtgctcgca gttgactatc cgctcgcttc gttcttggtt
    25681 aaagcataca gctgaaacag agggacgact cccaatggtg ccctcaccgg gagccctcga
    25741 ttccgagtcc gaacgcgcta ctcgacgagg gctctcgcgg tttcccttca agcatcagtc
    25801 acagctcgaa acgcagtcct cgtgtttggt ctcacacgag ccacgacagc cgtcgtcggt
    25861 acacgagcca acacaggttg ttttagtgct gtcgcacttg gtgacgcagt cgtctttatc
    25921 accgtcttga ccatcttggt caatcaccgg gtcatcgacg ttggccgtgc aggcggtcgc
    25981 ggagagcccg agcgcgaagc atgtcaggga gatgccgata aggtgcttga tcatggatgt
    26041 tgctccttgt gctgactacg tctgtgtcac gcaatccgca ttctcgaaga gcaaactgag
    26101 tgccacgtaa atgggccgag cgcattattg gattccctta ctctcttagg tgttgattgc
    26161 cgtgctttgg cccatgtgct ggtgacaatg tttatggttg gccccgaaat gatacgttca
    26221 attctgagaa tccggtccgt ttgtccgcac atccagcaaa ctaagcacgc cacaccgctc
    26281 ggcagcgcac gggtagtggc gttcggttgc aaggctgtca cacggcccgg ggtgccgcgc
    26341 caatccggtg agacaatcag gagcgatcaa tagccatacc cagtccggtt agctgtgagg
    26401 ggtcgtcagc caagttttcc agcatgaggc gcagtgtctt caccaggcgt gtttgatcgg
    26461 gctccggaat gcctgccatt gctcgggtca cgacgtcgct catggccgct ttggcgcgcg
    26521 cgagcgccgt gttcgcgcga gaagtgagcg ttacgagctg agctcgcgag tcattggggt
    26581 cgggcttgcg cgtgacgaga cggtctcgct ccatgcgttt gagtagcgcc gccatcgtgg
    26641 gctgttcgac atgaatgaac ttgagcagag ccttctgttg cagcggcccg tttcgctcca
    26701 gtgtgatcgc cacgtgtagg taggccatcg agaggcccag gggccgcagc tgatcttcga
    26761 aacgacgcat cagcagacgc gacgcctgat tgacccagaa tgtcgggaag gacgctgggt
    26821 cccagtccac ttgaccagat tccatagcca gctatatagc agcctatgta aagcgcggcc
    26881 agcccttcgt cgatcgccat gttggctcga agagggctcc gcggagagcg tcatgaaaac
    26941 atcgattatc aacgtccgtg tgttcgacgg tgagagcgtc cgtcccgagg catccgttca
    27001 tctgttcgaa gaccgcatct tgggcctggg cgggcaagct ccgtccgact cggagattgt
    27061 ggatggcaag ggggggacct tgttgccggg gctcatcgac gcgcacgtgc acaccgacga
    27121 atccggtttg gccttggcgc tccagtttgg cgtcacgacc gagctcgaaa tgcagggcgt
    27181 caatacccga aacgatcgga tgcacatcac ccatgacgac actctcgccg atgtacgttc
    27241 ttcgggtttt gcgctcactc cgccgggtgg gcacccgagt gagctcttcc cagaagactt
    27301 cgatccttct cggcttccgc ggcgacgaga gcgagagcgc gtgagcccgc tcgttcaggc
    27361 aaaggtttcc acaccgcgcg aagccgaaac ggtgattcca gagcttatcg ccgggggctc
    27421 ggactacatc aagttcatga ttgatgacgg ctgcgtcgag ggccacccag gcttgccgat
    27481 gctggattac gcgacgctga gcgcgggcgt ccgcgaggct cgcaagcgcg gcatgctcac
    27541 cctcgcgcat gcattgaccc tggacgccac acgcatggca gtcgaagccg gcatccacgg
    27601 gctcacccac ttgttcatgg atgtgccgca cactcgagaa gtcgtggaca ctattgccag
    27661 atccggtgcg ttcatggtcc cgtgtgtcgt gctgaacgcc tcgatgatgg gcattaacgg
    27721 cgcttctctc gccgacgacc cgcgcgtcgc tccccatctg aacgaggcat gggatgcaac
    27781 cttgcgcagt agtttcaacc actatccgca agggaaattc gaagatcaga tggcctccgt
    27841 caaggccctg catgatgccg gcgtggagtt gctcgtgggc accgatgttt cccaaccctt
    27901 cccatttcta ggtgggctcg cgcacggcgc gagcgttcac cacgagcttc agtatctggt
    27961 ccaggcgggc ctaagcccca ttcaagccct gcgagcggcc accgcttcca cggcacggcg
    28021 ctttgggctg ctcgaccggg ggagaattgc acctggatta cgcgccgatc tcgtcctcat
    28081 cgaaggcgag cccactcggc aaattagtga caccctcaac gtacgaagcg tttggcgcag
    28141 gggtacacac gccgtgaacc gcccgagttc ctgttcttgg tgaggcacgt gaccgaaccg
    28201 aagtaccgga acgccgaggg aatgactgag gggaacgact ggagcgcgat tgggccgttc
    28261 cttcatcgat gtgctggagc gtctctcggg tcatactgat gcccttgtcc gtgaccgtca
    28321 gcagcacatg cggccccgcc atcgtgccga gcgcttgagg gcatgttgcc gtcgacgcgt
    28381 cacgtatcgc ctcgacgcgt cctagcagag aggtgagtgt ggcccgaagg cgatcgtgtc
    28441 atcgtccggg atcttacggg cagatggctg cggttcgatc attgcaccac ctgagtgccg
    28501 gtgcgccgaa ctcgcggctc ccacatggcc ccacgaaccg aatcggacgg gcttgcgtgc
    28561 catcgggagg cgcgaaggtc atttccccga aaggaaatag atatgtccca cgaacagcat
    28621 cgaagttgtc tggacgcctg tgaaaagtgt gtagaagcct gtgaacattg tgcgaccgcc
    28681 tgtctgggtg aatcgaacgt aaagatgatg gctgattgca tccgcctcga ccgggattgt
    28741 gcggatatct gcgcaatggc tagtgcttgg atgagccgcg gaagctcgtt cgcggggcag
    28801 ctgtgcgcgc tatgcgcggc cgtttgcgag gcatgcgcca aagaatgtga gcggcacgaa
    28861 gccgagcact gcaggcgttg cgccgaggcg tgccgcaaat gcgccgagga atgccggcgc
    28921 atggcggcgt gagcggcgag gagcaactta ctcaacgcag cagcgtcccg tgctctcgtt
    28981 ctggaaattg ctccgcccag cgtttccgaa gtgccgcaaa cgaaagagga tacttcacat
    29041 cgagcacccg gacctcgcgg agccggtcgg tgcggaaatg ccgaaaggct tcacgctcct
    29101 cgcagtgggc cacgaggatc cgaacctcat ccatgtacgc caacagaagt ggccagagcg
    29161 tgcgccgagt gagcttccca ttggcgtctt ggtaagcaat ctcgatcttc ctgcgctcgc
    29221 gaatcgccgt ccggatcgga ccaaggtcag tcgcgtcttc gaagcgggtt cgcgcgaggg
    29281 ccgggcgcag gctcgcgtcg aaaagcaacg gacggagctc cctgggaatc acctgactca
    29341 gcttttctag caggctccgg gcgccgtgcg agagcgcgga gtcacccctt tggaataccc
    29401 acatcaagcc gagcatggct gcttcgacct cgtccgcggt tagcatcagg ggaggcaact
    29461 cgtagctcgc ttcgatcacg tagcccgtgc cggcctcgcc gcgcacgggc acgccttggg
    29521 cgctcagctc cgccatatcg cggtaaatcg tccggagtga aactgaaaac tcgtccgcga
    29581 gccgttgtgc ggtcacgggc tgcttcctgc ctcggaggac ctggataatt gcaaagagcc
    29641 ggtcgttacg gcgcataggg cgaggctacg atgacactgc tgacatcctg ctgtcagcag
    29701 cccctcgcta cactcctcga atcctgttct tttgccccaa tttcggggag gagcaccagc
    29761 atgcaattcg cgtccacccg catcatcacc caagacgtcc agcgcctcgt cggtttctac
    29821 cagcaagtca cgctaattgc tccggtctgg cgcacgcccg agttcgccga gctgtcgacc
    29881 ccgacctaca ccttagctat cggcagcgaa agcaccgtcg agagattcta cccgggagcc
    29941 gctcggggct cgcgcaacca atccacgatc ttggaactcc gcgtcgctga cgtcgacgta
    30001 gagtacagga gactcgctga gcgcctcgac gtcgtggttc aggttcctac cacgatgcct
    30061 tggggcaatc gttcactcct attccgcgac cccgacggca acctcgtcaa ccttttcacg
    30121 cccgttagcc cagaggcgat ccaaaggcac acgcggggca cataaaaccc aatctcccga
    30181 gttacgctcc tcactcgagg ctgggtctct tcactagcct cgtcagggct ccggggctgg
    30241 ctagaccgga ttgcgcggag ttttgactga cgcagagcgc gagttccctt gccgtgtact
    30301 agctctcgaa caaactgggc actacctcag ttgctgtgcc caatcggcat tcatcgaaag
    30361 cggttgcatt ctcgggacgc tcgggaccaa catacacggt cttcgcgcca ttcgcccttg
    30421 cggcggccac gaatcctgcc gcgggataga cggaaccgga actaccaatc gtgaccatca
    30481 gattgcaccg agacaacgcg cgttcgattt cacccatgaa gaagggaacc tcgccaaacc
    30541 agcagatgtg cggccgaata ccggcaccgc agtcgcagcg tggcaattga tcggaattca
    30601 ggtatagcga ttcgtcgagg aagggtggag cgtgttcgcg ttcacaacga ctcttgaata
    30661 gctcgccgtg catgtgaacc acggcgggcg agcccgcatg ctcgtgcaaa ggatcgacgt
    30721 tttgagtgca gagaaacacc tggttctccc accgcgcctg gagcttggct agcgcgacgt
    30781 gtgcggcgtt ggggcgagtt tcacgggctt gtgcccggcg ctgggagtag aagcgccaga
    30841 ccagtgcggg gttttgcgcc cagccgctcg ggcttgcaac ttggcgcacg tcgtattgct
    30901 cccataaacc attggagtct cggaatgttt ggatcccact ctcggcgctt atcccggcgc
    30961 ccgtgagcac gaacaattgc gtggacgagc cgaaaggcag agcctcgaat tcgtgataag
    31021 gggcaacgcg catatgcaat cggaacatgc tctcgcgccg ctcgtcaaca tttcgcgacg
    31081 tcgcacgcag agactctcgc tatcaatgct cgctatcaat gcgcaaggca ccgggagaaa
    31141 ccagtctgga acgatatcgg ctctcgcggc ttgagcaatg aggagtgggc ctggatgcgt
    31201 tctagcatcg aggagatgtc gaagacctga gctgggcgac aattcaatct agatttcgcg
    31261 ccgacgacgg acgaagtccg caaggccaaa gcgagagcta tcgcgacccc ccccccgggc
    31321 cgccatccga actcccaccg acgagtatgg aactctatgg ggttgggctt gcaacccagg
    31381 tcatcgtgga gcccgcgacc gtggttaagg tggacgcgac catccgtccc aaagtcgtat
    31441 cgaaggtgtc tctcacgagc ccgttcgtgg accaaatgga atccacgtaa atcaccgtgg
    31501 gattcaacca cggcccggcg gtaccggact cgacttcgat cgtgagctga taaagatttg
    31561 cgggatcgaa tggatcgctg aggttgccga cggggatctc aaggtcagcc cagccttcgc
    31621 tgaacctctt gagaggtacg gtgacgccag ggcccgggct ggaagccgat gaatccgtcg
    31681 gatacacgaa gagcgaacca ccgagcgcgc ccggcgcata tagccgcatc ccgaaggcgg
    31741 agttgctgcc gtctatgttg gttgcaaact tgttgccata gcgttgaacc tgagccgccg
    31801 ccgtgaaggg gatcgtgagc ttcacgagac cttcaggcgc cacgcagctg ccctcgttgc
    31861 aaacctggtt gcttgcgcaa gcagtgccac tgctcgcaca gtcggctctg cacgcgagga
    31921 gcgagttacc gaacacttcg cagccgtcgt cccgtctgac gccgtcgtcg acattgcaat
    31981 cgaggaaccc tggcgcgcac gcggggacgc acttcgagtc cgtgcatgag gcggcggcca
    32041 ccccgttggt ggcgcaggta tggtcacaag ccccgcacga tgcgggtgac gcgaggttcg
    32101 cttcgcagcc gtcatttgcg gcttcagcgt tgcaatcggc gaatccggtc gagcaagcgg
    32161 gctcgcatat cccgttcgaa caggctgagc tgctcgcgtg attcaccgag caggaaactc
    32221 cgcaggcgcc gcagtccatg acggttttgc ccgatggatt tcccaccgtg agatccgttg
    32281 tgcactccgg gctgcccgca cacgtgacga gtcccgtggc gcattcattg taagtcgcgc
    32341 cgcccgcacc cgcgccgccg cctgacgcca tgccagccgc gccgccctcg acagtgccgc
    32401 ccgtactggt gctgtcttct cctccattac cgatcgaacc ggcgctgtcc gcgtgggtgc
    32461 caccttccga cgtgcctccc tgcgcatcat cgactgctcc accgttaccg ccgccggtgt
    32521 tgcccgactt cccgcccgta ccgctcgcgt tggcgctccc acccccgctc ggcgtgccgg
    32581 aactcccgct cgagccgtcg tctcggggct cggtacgagg tgtggccaag caccccgcgt
    32641 acgctgcgag ggtgaaaata ccgagcccga acacgcgttg agccaagata tgtcgatgct
    32701 tcttcggatt catctaagtc ctctcactag cctcccatct tatccgtatg cggtgttggc
    32761 gtcggggata tctcgttcat tctctcgaat tgcaccccaa cgggtacgcc ggcctcgcac
    32821 caacgccccg tgacgtcagc tcgacagtgt cgggcgtgcg accgtcgtcg tgaaggttcg
    32881 agcgcccacc gtcacgctca gctcgccatc gttgagcagc accatccacg atgtggtgcg
    32941 ctcgaaagtc ttggcgagct ccgcgacgaa ctccggtttt agtccgaaga tgcggaggtg
    33001 ttcggctcgg tgcaccttct tgcgccccag ttctttcatc agcatttcca gatccttgtg
    33061 acacacgacg ctaacgcgct cggctcgctt gctcgcgagg tgcagccgct ctgccgacgg
    33121 caatcccacc tcgatccaat gcagcaacct gccagtcaaa tcatgcgtcc agagcgccgg
    33181 ctcttccgtg tcggacagac cctttccaaa gtcgatgccg tcctcgagac aaattgcgta
    33241 cgcgagcgca cgcgccacga ctcgatcgag ggcctcggag ggatgctgcg cgatacgcag
    33301 atcgaggtct tgataaacac cccgatcaac atccgaaagt tcgaggtgga gtcggtaaag
    33361 ggttgaggtg agagccatcg tcgggcagca tattccaagc tgccccacgc cgcgagcgcc
    33421 tcgcccacgc ccgcgtggaa gcatgctcca aatcgtggtt cggcggcgcc ccatcggcgc
    33481 cggccgagcc gcggtttcaa ctcggcaatg cgcactcacg ccattgtccg ctcggtcttt
    33541 tcggcctcct ctcgagcgag atcaagcgaa gcagagccat gtatcgcttg acgtgaagaa
    33601 ggactgcctg tagcccagtc caggtgggag ttgcgactcg tcgtcgcgct cgattggtgt
    33661 gaggagaact tgatgaatca ttcgatgggt cgtgctccga gagtgcacag atggggcaat
    33721 ttcgcgtggg ggatcgcgct cgcgattccc gcctgttccg gcgaaacgac gcgggcgggg
    33781 gaatctattc agcgggtcac tcaggcggcg gtgacgccga ccaaaataac caccttggct
    33841 cagctgcggg ccatggtcgt gaccggtagc tatgagctcg gaagtgacat cgatgcctcg
    33901 agcaccgcca gcactccttt cgtgccgatt ggcacgcaag cctctccatt cagtggcttc
    33961 ttcgatggga aaacgttcaa gattaacaat ttgaccatcg ccggtaaccg caattatgcg
    34021 ggcatgttcg gttacgcgag cggcgccacg attcagaacg tgaagctcaa cagcgtgaaa
    34081 ttgagcaatg gaagctcgta cacttacacg ggtgcaatcg tgggttacat gacggcaacg
    34141 aacctctatg acagccaagt cagtggtacg gtgaccggtg gcagcacgac cggaggcctc
    34201 gtcgggtacg ctacgggagg ttcgaacctg attggaggca gcgttacatc cctgagcgtc
    34261 aacggcggtt cttacgtcgg aggtctggtc ggatacttga ctggttcgtc ttatgcgatc
    34321 aatggcactt catcgggcgg cagcgttacc ggtgctgaca gtacgggggg catggtgggt
    34381 cgggctagta gcggtagctt gagctactcg aatgcgacgg cgctcaacgt gaatggcacc
    34441 accaacacag gtgggctcgt cggctcgttg acgagtggaa cgatagccta cgactcggcg
    34501 acgggaactg tgaaaggcac cgatagaacg ggcggcgttg ccggcgcgat gaccgaggga
    34561 atagccatct tcgactcggc cacgaacatc accgtcaacg gcgcggcgga cacgggcggc
    34621 ctcgtcgggt tgctcacgcg ctcgacgatg gacacaggaa atgtcagcgg cagcatcacg
    34681 ggtggggcca acacgggagg tctgataggg cgaatgatcg ggacgctcgc gaggcgatcc
    34741 gtcctgacgc tcgcctacat agacgaaaag agtgcgggta acccgagcgt ggtgacgggc
    34801 ggtacaccgg tcggcatggg agtaggatcg gtgcagagct acacggacct tacgaagagc
    34861 tacgcgatcg gcaaagtgac cggctcgagc acgcgcatcg gcggcttcat gggtgagatc
    34921 aacgcgacgg gaatcgcgct cgccaacgac gaacctcgcg ccaatgtcga agagatcttc
    34981 acgcaagtgg aggtgacccc agcattcgat aacaccacca gcaacgtctt tgccggcggt
    35041 ttggtcggga ggctgctcgg tgcctcaatc aagaacatca acgtggcggg atcggtaaag
    35101 gggcgcaact acgtaggtgg agcgatcggc tatgccgtca acgacggcgc gaacgttacg
    35161 ggctctatct tgagatccat tctgacccgc ggagaggtca ccaacatcgc cgtacagaat
    35221 cgttcgggaa ccatcggcgg tgtcagcagc aagttcatgc gttgtggacg tacctattgg
    35281 gacactacga cggacggtgg cagcgcgccc cctttaccgg ccgacgaaga cccgagctgc
    35341 gaggtgggcc tcacctcaac ccagctaaaa gcccccgaaa agctgtacgt ggacgccgat
    35401 catcctaacg gcaactacga tatcttcaca tacgggatga tcatcgatcg agaatatcaa
    35461 gcaacgtggg gggctttgaa ctgccagctc ggatccggga gcgacggtga tttcgggttt
    35521 gcgttctgct acggcgtgga accgtcggcc gagccccccg tctgggtcat gaactcgggt
    35581 accgagtaca acaccctcgt gaacattccg cgtccggaac gccagcccaa gaactgacgt
    35641 tccacttccg agctgctcca gactcgatac atctcggtct ggcgcagctc cgtgcgcggg
    35701 gatgctttca gtcgcctctg aactcgttgg aacgcactcc ataccgcttc agcagtcgat
    35761 gaaggctctc ccgctcaatt ccagctctct ctgccgctcg ggtcacgctc ccactgaact
    35821 cctgcatcaa agcgacgaga tattcccgac acgcgcgctc acgggcttgc tcgagcgcct
    35881 cgcgatacgt gagctgagcc agacccgaga agcttcgacc cggccctgca ccgcgcacct
    35941 cgtcaggtaa ggcctccaac ggaatgcgcg cctcgtcgac cacggccagc gctcgttcca
    36001 cggcgttttc gagctctctc acgttacctg gccacgagta ctgcacgagt gcggaaagcg
    36061 cttcgggcgt gaacccctcg actcgactct gatacgcctt gccgtgctgc tcgatgaagc
    36121 ttgcggccag tagcggaatg tcgtccttcc gatcacgcag cgcgggaagg tgaatgttga
    36181 agacattcag ttgatagagc aggtcttcgc gcagagctcc ggatttcaac aaagccttca
    36241 cgtcattcgc gctcgtcgcg acgatccttg gtctgagtgg actcggttcg gggtccgcgg
    36301 cgcgcggccg ctgctggagc cacagacaga gtcgggcctg cagtggcatc gggagttcat
    36361 ccacgttttc cagcaatacc gtgcctggag ccgcggacgg actggtcatc tgtcccgatg
    36421 attgaacttc gacggcgtcc ctctgggcaa agagcccctc gctcaattcc gcaggattgg
    36481 ccgaacaatt gacgatcgaa aggcttccgt cgcttcgagc gctggcccgg tgaatcccgc
    36541 gagccgccac tcgtttgccc gtcccgcgtt ctccggtgat caacacattc acgtcggagt
    36601 tcgaagcctt ggcgagcagc cccaatacgc gcagcatttc ggggcttcgt ccgcaaaggt
    36661 tctcgaacgt tcgagaggcc tggagcgcag cccgcaaatt gctagcctcg aggcgcaatc
    36721 gtcgccgctc cgccgctcgc tccacgacca ggagtgcctc atccggctcg aacggcttgc
    36781 tcaagtagtc gtaggcgccg gctttcatcg ccgccaccgc gcgctgtacg gtgccaaatg
    36841 ctgtcatcaa gatcacttcg atatctggcc tggcctcttt gagcctcgta agcagttcga
    36901 agccgtcgat ggccggcata cgaatgtcag atatcacaac atcgaattcg gtcctcaggg
    36961 caagagcgat cgcgcgcagg ccgtctgtct cggtggtaac ttcgcagttc ggcgtaagtg
    37021 cgtccctgag catccgcact acgctctctt tgtcatcaac gattagaacg cgaggctgca
    37081 tgatcgactt ctggtttgct gttcgcggag gggagcctga aaattgcttc tgttccaact
    37141 tcggatggcc gtaactcgat cgtgccatcg tgagcgtgca caatggcctg tgatatagcc
    37201 aggcccaacc ctgtaccctt ggccttggtg gtgaagaagg gctcgaacgc tcggtcgagg
    37261 gtggctttcg tcaccgctgg tccgttgtcg cggactcgta cttccaccca acctgggctg
    37321 gagtagctct cgatcgtgat cgtgccggtg agagtggtcg cttcgaccgc gttttgaagc
    37381 aaattcaaaa tcacctgccg aattgcggcc gcatcgccca atacgtagag cggtgaaggg
    37441 ttgggcgcaa tgttcacggt tctcccggcg agtttttccc cgtcggttag acgctcgaca
    37501 ctctcgcgga gaagctgatc catgtccacg ggaccacgga tcccggccgg aggacgagcc
    37561 aactccagta agccctgcac gatgcgccgg catagctcga gttcatcctc aatcacctgt
    37621 aatgcgtttg ggggagggct cgggcgctgc aacagtcgaa tgtatccgag cattacaccg
    37681 agaggattgt tgagctcgtg cgcgaccccg gccgcgacct gtccgatgac ggccagccgt
    37741 tgcgcttgga tcaatctgct ttgatggttg gtgagcgacg cggccatgtc attgaaggtt
    37801 gcggcgaggt cggcaacttc atccgagccc tgtactggaa cacgggcgtt caactctccg
    37861 ccagacagct cgatcgccgc cgtgcgcaga accgagaggc ggcgcagcaa cgactgtcca
    37921 atgatcatcc acgccacccc cgtcacggca atggcgagtc cgaaacacgc gatcacgacc
    37981 aaagcggctc ggcgcccgag ctgctctttg gcgtgtcccg ctcggattgc tcgggtctcg
    38041 agcatcatgt tcagtgactc actgagcgcg gcaacccgcg cccccagttc ttcgttctgc
    38101 tcgtggagct cgcgaactcg cgcgtgcgct cgtcggtcga cctcggggag aacctgttcg
    38161 gtgaactctc ggtccgcgcg cgtcaggagc gaggaaattg cagccgcttc gccgcgttcg
    38221 atgtcggaga gcggaagtcg aaggagctgt tctacggcgc tacgcgcctg cgttgcagcc
    38281 gcccgatagc gcgccaagtg agagcgatcg gcttcgatga tcgtgtgggc ctggtgaatg
    38341 tactgctctc ggatcaggat cgaaacgttg tgacccgtat gcctcgcgac atccagagcg
    38401 gcagactccc gttcggctcg tgagagctgc cggaatgagt atacggtggc accgagagcg
    38461 gttccgagta gcgcgatcgc catcgcgaaa acgagcgcca ctcgttgagc gatggagcgg
    38521 gccggacgcg attcttgggg gagtatgtcg agtgccacga aaggaatcca tcaagtatat
    38581 cgacgtgccc tcccgtgata gtgactccgc aggttacaga tggctaagaa cctataccaa
    38641 taggagctcg tcgcgggcgc tcctactggg atggttctaa agaaccctca gagatgcagg
    38701 gactgagcgg atggaatggt acgtgctttc gtgccgttga ggcaccgggg ctgtttaggg
    38761 aaaccgatgc gtagcacgga tcttggttac gctcgccgca gcagttgtcg cacaatggtc
    38821 ttcgtcttgc ggtaagccgg tcgcagtggg ctcttgagta gaaccaaatc ccgcactccc
    38881 ctcgccatgt gcgttacgga ggtgggatcg acccactggt acttcgtaga catgaggtcg
    38941 aggctctcga gcggctcgaa cccggcccgc tgtaaggtcc accggagcgg taggtcgtct
    39001 gcatacaagt gccaggcgtt ggcttccgac ggagggcgcg tatcgaagtt cgcgaaaagt
    39061 aaacctccag gtttgagcgc gaggtgcaac atttcgagaa ctcgcgggag gtccggcacg
    39121 tgcacgaggg tgtcaatcgc ggtgattaca tcgtaacggc gcgcatcgag ggcgctcttg
    39181 ttcagatcga tgaacgtgac tacgtcaccg cgtcgatcca gccgaaattt agcgaattct
    39241 tgcagcgagc tggaaatgtc cgcgagcgaa ctcctgtacc cgagtctgga aaagagctgc
    39301 cctgttgcac caattcccga tccgaagtcc aggtgttcag gggcatcctt cgccggcgga
    39361 acaccttccg caatggccac gctcaccgga taggcgtagc cctccgcttg tagataggcg
    39421 taccagagca gatcgaagct ccacgatttc gtggactggt agaagctcgc gatcccgtcg
    39481 tcggtgcttc ggtccccgtc cttccactcc tccgcgctcc atttctccca atgcagacac
    39541 cgctgtctgc attcctccgc gctatagcca aagaaggcgc tcaaatcatc gagaactgcg
    39601 tctcgcacgc tttgagtgtg agggaggagt agcgattctc gaaatcgcgc catgctttga
    39661 ctggcgaagg ctcgtcgccg tcgtggcgta aatgtgcccg ggtccgggcg ttgaaaggat
    39721 ggatccaagg ggtcgatgtg aagcccttgc ccaaactcga aactaccatg catggggcat
    39781 atcctttgcg gaagggtgga ggatggacgc gagaagacaa aaccaagccg gcgctcggtc
    39841 gtgaggtttg acggcgtcgc gaggcagcac gcgtttaggg tgcgcccgta cgtaccggcc
    39901 gtaacaatat ggcgcggaag gtgaggatgg gtccgtatcg attcaggtcg agatccggct
    39961 ccgaaatctg ctcgtcatga gacgtctcga ttctgcagct cgcaaggcca agtcgagagc
    40021 ccctgcatcc ggagactctc ggctaagtaa gagccagaac gctgggtgtg caccagcgtt
    40081 acctacatac ctatatcgag tgaagaatca aagccctcag tgggtattga aattctttat
    40141 ggctgcgggt tgtggccttg ccggctcggt tcccaatgcg agtaattggt tgcgagaccg
    40201 gtgctggttg cttgcagcta gctcctgtat ctcgcctggt tacggctcat gagtgtaacc
    40261 tccgtggttt cagactcgac cccaaccgcc tacgtcgcgc ggagcgaagc ggcagacacg
    40321 tcctaggctc gccgtcgatg cagtgcgcca gccgagctta cgctctgagc cctgacgtga
    40381 tcaccgcgac tagtctccgc accgcagcct tactcgcgag ctccctcgct gcggtcagag
    40441 catggagaca ataccacgcg agctcatctg cggagctgtc attacggaaa tcgccgcttg
    40501 cgatcccatc ggcaatcaac tctttaatga acttccgcaa ccgctcctga gctcgcgcga
    40561 cgtgctctcc ctgatgtagg agaataccca gctccgtagt ctgctgctcg cgagtgatca
    40621 tgccatatgc ttccagaacc gccgccaagc gctgcgcggg attcccagcc tgagtgccgg
    40681 tggcgacgag ctgctccaag tgagcgttta tatgctgctc gtgccaagcg cggagaattc
    40741 cctccacatc ggggaagtat ttatagagcg ttgctcgtcc aatcccagtc tctgctgcaa
    40801 cttgcgacat ggttacgccg cgcaacccgt gtttcgcgac gagcaacgca gtcttgttca
    40861 gaattgcctc gtgtacctcg cgtcgatgcg cttcgattgt gtcgttccag agcttgggca
    40921 cggccggagt atacgcgagc actacggaag agacagcgtg tacttgacca atacagtgtg
    40981 tatcgtaaac tggtcgctgg atatgaaaga ttccgatgat tctgggcgtt cacgtcacga
    41041 gagagtcggg cgcgtcgagc cgccgaagcc cccgccagcg ccgcgctggg tgaaagtttt
    41101 gggcctcgcc gcgttggcgt ttctattggt ttttctgatc gttcacgcgc tggggggagg
    41161 actgggccgg cacgcgacgg gggggatgca tcaccgagag cggagcggcc cgtgatggct
    41221 cgttcgctaa ggagtcttgg ccttaccggt cacgttgcgg cgtcagtggg ctggcttggt
    41281 gcggtgatgt gcttcgacgt gctggcgttc atcgggcttt acggttctga cccgcagctg
    41341 acgcgaagct ctctcctgtt gatgagaccg atcgtcgaat atctcatcgt cccgctttgt
    41401 ctcgccgcga ccatcacggg agtagttcag tccatgatca ccaagtgggg tctcttcgaa
    41461 cactactggg tagtagtcaa gttggtattg accatcgtgg cgacgctcgc tttgtggctt
    41521 catctgtcac cgatccgcga cgtctcggcc gcagcaaagc ttgcccccgc gctgcccagt
    41581 agcctgagcg caacgcgcct ccaggtcagc gtagacgcga cgttggccac aataacgctg
    41641 ctcgttctca ctggactggg cgtctacaag ccacgcggag tcacgcctta tggttggcgc
    41701 aagcacaacc tgcgctgggc gcattccaaa tcgtaatcga gttgggccac ttggctgcga
    41761 gccgcagcgt cgttcgggca gacctcctcg ccttcaccgg gattgccggc gaattccgcc
    41821 tggtctccgc gtcacacggc tggcgcggcc agagccgctc gcagtcgcga aacgcctttg
    41881 gagcggaaac cgcgggatgg cagccctgct gcgtgacggc gcttgccggt tcaagtagcc
    41941 tggccaggcc aatttgcgag gggatgatga gcgcacctca gacgagcaac atcctggtcg
    42001 tcgatgacaa ggtcgagaac ttgcggcttc tggccaatat tctggagtcg ctcgactacg
    42061 aggtacggcc ggccacgagt ggacgcagtg cgctgctggc ggccgaacgc gatccgccgg
    42121 atctcgtgct gctcgatgtg aacatgccgg atatgaatgg gtacgaagtg tgcgttgcct
    42181 tcaaggcgcg gccgcggctg aaagacattc cggtgatctt tctaaccgcg ctcaacgaag
    42241 tagcggacaa ggtgaaggcg ttcgccgtcg gcggggtcga tttcgtcagc aagccatttc
    42301 acatcgacga ggttcacgcg cgtgtgaaga ctcacctcga tctgcggcgc gctcacctcg
    42361 aactcgaggc cagttacgag aagctcaagc aactcgagca gctgcgcgac gatctggtgc
    42421 acatggtggt gcacgacatg cgttcgccgc tcaccgtgct tggcggacac ttggcctgcg
    42481 tgcaggtgga agcaaacaga ctgagccgcg aggccaccga ggacctgcgc gcagccatgc
    42541 ttggcgtgca ggcgctgacg cgcatggcca atgacttgct cgacgtgagt cgtctggagg
    42601 aaggcaaact accgctcgag ttggccgatc atgacctggt cgaaatggcg gccgacgtgc
    42661 agagcgcgat gcgcgagttc gaacggggta agcggctaga gctgttggcg agcgaaccgc
    42721 tgcacgtaaa ctgcgatcgc aatgtggtgc ggcgggtact cgaaaacctg gtgagcaacg
    42781 ctatcaagca cgcttcctcg accggcgcag ttcgcatcga gctcagccgc acgccgacgg
    42841 gcgctcggct ctgtgtgtgt gacgacggac ccggcgtgcc ggcccaagcc cgccagcgta
    42901 ttttcgagaa gttcggaacg atcgctgcgc gtcgagcgaa tggctatcac tctgcgggcc
    42961 tggggctcgc gttctgcaaa ctcgcaatcg aggcgcacgg cggtcgcatc ggcgtcgaag
    43021 atggtcgagc gggggggagc cgattctggt tcgaacttcc gcgcgaaccc tgatcgatca
    43081 atcgaacttc gcgaatcagt cgtcgagcgc acgcaccaga tcttcgtagc gatattctcg
    43141 cgcgagctcg cgcacctgaa tcgccgcctc cgccgataga acgctcagcc gttcggccac
    43201 gctctcgatt cggcgggccc gcgcctgaat cgccgcctcg cgcagctcct gacgtagagc
    43261 ggtcggtacc ttctgcagaa gcagggaaag cggccgcagc gggggatccc gcgtgtgtgg
    43321 cgcgcgtgaa tcgccctcgt agagcagctc cacgcctagc aatcccgcca ccatctcgag
    43381 tagctgtgtt tctcggtacg gcttgaaaat cacctcgtgc gctccggctt ggcgcgctcg
    43441 ttccgcgagg tcttcgaagc cactcgccgt gaacgcgacc agcaccgcgc gagtgccggc
    43501 ctcctgcagc cgccgaatcg cttcgagccc gcccatgcca ggcatcttca gatccatcaa
    43561 gacgagatct ggcttccagt cgtcgtggac ggctatcccc gcttcgccac tgttggcgct
    43621 ttgagtttcg aagccaatcg tgccgagcag cgtggtggcg acgcgaacgt tgtcgggaac
    43681 atcgtcgatt accaagatct tcttctgtgg tcgaccggga accaacccga tgatccgccc
    43741 ccgtcggagc ttgtctagcg cttcgtcgga tgccgctgtg gcctcgaaaa cgaacgtgaa
    43801 ggagctgccc actccaaccc ggctcgtcgc ggtcacgtcc ccattcatca aacgcgctaa
    43861 atgccgacca atcgacagac caagcccggt cccgccggcg ctcgcgcccg ctttggcctg
    43921 ctcgaaggtg ccgaagattc gttccaagtc ggccgtttcg atgccgcaac cggtatcttc
    43981 gacgatgatc ttcagcgtgt gccggtggtc gcaggaacgc tcccgcgtcg cccgcagact
    44041 cacgccgcct ctggtcgtga acttcaccgc gttgctgagc agattgatga ccacctgccg
    44101 caccttgccc ggatcggcct cgatctgcgg tggtagatcc ggcgccagca cgaattccag
    44161 actcaggccc ttggcattcg cgagcgcggc aaacatttgg tggacgccat gaagcagact
    44221 cggaaggtcg aacggctcgg gggctagctc ggcgcggccc gcctcgattt tggacatctc
    44281 gagcacatcg ttgagtagag tcagtaagtg ctcgccgctg ctcaggatgg tttcgacttg
    44341 cgctcgttgc ttctcgttca gctgcgtatc acgacgcaac agctgggcgt agcccagcat
    44401 ggcattcatg ggcgttcgaa tctcgtgaga catagttgcc aagaacgtgc ttttggcgag
    44461 actcgctcgc tcggcctcct ctttggcttg ctgcatctcg tgcgtgcgta acgagacgag
    44521 ctgctccaga tgctcttggt gatggcgcag ctcgtccaaa agtctccctc tctcttcctc
    44581 cgctagcttg cgcgcagtga tttcggcgtt cgctcgtgcc aaggaacgac tgagcgtcag
    44641 cgtaatctgc gggtgcagca gcacggacaa cgcaattgcc atgcagctgt aggcccagcc
    44701 aacgagaggt gtgaagttga gttgcgacgg tggcaatagc ccgttcacct gcaattgcgc
    44761 aagccaatat ccaattgcgg ccaacccaat ccccgtcacg agcccgccag aggtgccgag
    44821 cagtgtgcca gccgccatgc aaatgtagac gaacaagctc agcgccggcg cgctcgcccc
    44881 gcccgacgta taggccctcg ccgtaacgat actacccagg ccaatcacga gcgcccaact
    44941 cgccaggcgc acgtgcccgc gcccattcag ccagagcaac ccgatcacga gcgccaacag
    45001 aaagccgagt gtgcggaggc gtcgtcctgc agtctcgggc tgggctagca tgagcagcag
    45061 caagaaggca gcgatcacca cgatcgtcca gcacgcaacc ccatggaaag tacgcgcgcg
    45121 ctgcgtgcgc tcttcgtcgg cgaaagtcgg ccagaaggag catcgcagcc acaatccctt
    45181 cagcgtcctc accgtcgatt ccaccctttc cgagcatgcg ccgaggctga tgctgcccac
    45241 gacatcgcaa gactaacccg ggctcggtgc ggcggggtga aattgccgcg ccccggaggt
    45301 tcgacaatga cgccgtcgat atgacaacgt tcgatgatga gctatcggcg ccccgtggaa
    45361 tctgtcggga ggcgtcacgg cagaggcttc tccagagtgc gacgttcgtt caggagggag
    45421 gggggcccgg agaggataag tgcgcgaccg tgcgatgcgg cccccgctag cgcagtcgcc
    45481 tcctcgtcaa ggcaagtccc gcccaccgcg ccggccccaa accgcaaagc cgtcgcgagt
    45541 agcagctgaa gcagggaagg gctccaacgc gctgggagcg gcggtccgtg ctgcagtcga
    45601 gccgccaagg cgcttcgtca gtcatcgcgg ctccgcgatc cagagcggtg aaaatccgcc
    45661 ccgccatcag ggcatgcgct atgtatagcc aacgatggat cgca
//
LOCUS       DASTKU010000912         3709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_400895, whole genome shotgun sequence.
ACCESSION   DASTKU010000912 DASTKU010000000
VERSION     DASTKU010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400895"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VFQ61_32140"
     CDS             <1..1161
                     /locus_tag="VFQ61_32140"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9959199.1"
                     /translation="MMADNIEHVISYWLMFQKFHSAALGGFAVVSHWLPYLLFSVAVG
                     ALNDRFDSRRLIQLGAGLFMLASLGWGYFFFTDTLRVWHAMVLLVLHGSAGVFWITSS
                     QMLLYDIVGPERLASAVRLNASARYLGVLVGPAVGSAIMLTLGSVRGIFLNALFYLPL
                     IVWLIRAPYGRHLRGGGVGPKRAVRGLADIAQALREARAMPSIFAMVVLTGAASFFVG
                     NGYQVQMPGFAQDLGHGDPGVAYTSLLAADAAGALVAGFLLESRGGLLPTRTSLALAL
                     SIAWAASLVGFAFSRIYLLALPLLFSAGFFELSFNSMAQTLVQVNAPDATRGRVLGLF
                     NMSAAGLRTFSGLTVGLAGSYAGIHLSLAASAAVFAFVAAGLLVRSRSPKLT"
     gene            1235..2236
                     /locus_tag="VFQ61_32145"
     CDS             1235..2236
                     /locus_tag="VFQ61_32145"
                     /inference="COORDINATES: protein motif:HMM:NF018659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HET9959200.1"
                     /translation="MDDARSRFGPIRLRCSALVCVAACLTACGADTERSGSASGDRAN
                     AGSGGANGGRLGNGGSGGGRPGTAFAEYVPEGPPVALDGVVNASDIAWKPTTDTFFVL
                     ADRVSELYEYEPDFRARRRTIALVNPPEDAEGLSYVRSLGTSDRLVLSTEAPTNMAWV
                     FDLDESATTLDFATAVRQRYEPIGASKVPNRGLEGVALRANEDGTQNWLYVCEEGEPQ
                     REPIRVLRFAYDEGGQALETYRDGSLSVEEPFVASALFDSAVGDLSGLHYDGATGTLL
                     ITSHLGSRLLRVDPDSGQVWDQMLLTRSPQYEGVTLASGNRLVVVSEPNWVEIFRGR"
     gene            2383..2832
                     /locus_tag="VFQ61_32150"
     CDS             2383..2832
                     /locus_tag="VFQ61_32150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959201.1"
                     /translation="MRLWQKGLSLTGALACLGLVACEQSPHAASAIDSNPTKARDHVI
                     GCFKDAYQAKQGTSPVVNNNERSAAEQLLSRYNGTSSRACEAINEAIGHNVCGLPMSL
                     VSIVQCDQRQREAKAGQGVDPNTKAQITDRIKENSRKDMSPNGMSGN"
     gene            complement(2933..3661)
                     /locus_tag="VFQ61_32155"
     CDS             complement(2933..3661)
                     /locus_tag="VFQ61_32155"
                     /inference="COORDINATES: protein motif:HMM:NF014113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959202.1"
                     /translation="MQGSGGATQCTPPTGGSNGYTTRFWDCCKPSCSWPNNTGGKQPA
                     KTCNQQNQSTASSTESACSGGGGFACWDMAPWAVSDTLAYGYAAFNGSQCGLCYELQF
                     TGQSNSQANDAGAAALCGKTMVVQVINIGNITQGQFDLMIPGGGVGQFNACTQQWGVS
                     GSALGEQYGGIQLTCQKQNNAWAARKSCTETACNNLFKGSNQSSLLAGCKWGVEWLQA
                     ADNPKVVYKQVSCPDALTSKSGLR"
BASE COUNT          587 a         1166 c         1227 g          729 t
ORIGIN      
        1 atgatggcgg acaacatcga gcacgtcatc agctactggc tcatgtttca gaagttccac
       61 tcggcggcgc tgggcggctt cgcagtggtt tcgcactggc taccgtacct gctcttttcg
      121 gtcgcggtgg gcgcgctgaa cgatcgcttc gattcgcggc gcctgatcca gctaggggct
      181 gggttgttca tgctggcgtc gctcggttgg ggctacttct tcttcaccga cacgctgcgc
      241 gtctggcacg cgatggtcct gctcgtgctg catggctcgg cgggagtctt ctggatcacg
      301 tcgagccaaa tgctgctcta cgacatcgtc ggtccggaac ggctagcgag cgcggtgcgg
      361 ctcaacgcaa gcgctcgcta cctcggcgtc ttggtcgggc cggccgtcgg tagcgccatc
      421 atgctcacgc tcggctcggt gcgtgggatc ttcctgaatg cgctcttcta ccttccgctc
      481 atcgtctggc tgattcgagc tccgtacggg cggcacctgc gcgggggcgg agtgggaccg
      541 aagcgcgccg tccgcgggct cgccgacatt gctcaagctc tgagggaagc gcgggcgatg
      601 ccatcgatct tcgcgatggt cgtgctcacc ggagccgcgt ccttcttcgt cggcaacggg
      661 tatcaagttc agatgccggg ttttgcgcag gatctcggcc atggcgaccc tggagtcgcg
      721 tacacgtcac tgctcgccgc ggacgctgcg ggcgcattgg tagccggctt tttgctcgag
      781 agccgaggcg ggctcctgcc gacgcgcacg tcgctcgcgc tcgcgctgtc gatcgcgtgg
      841 gcggcatctc tggtgggttt cgcgttctca cggatttatc tgctcgcgtt gccgctcttg
      901 ttctcggcgg gatttttcga gctgtcgttc aacagcatgg cgcagacgtt ggtgcaggtg
      961 aacgcaccgg atgcgacgcg ggggcgtgtg ctcggcctgt tcaacatgtc cgctgccgga
     1021 ctgcgtacgt tcagcggtct gaccgtcggt ctcgcaggaa gttacgccgg cattcacctc
     1081 tcgctcgccg cttctgccgc agtatttgcg ttcgtcgctg ctggtttgct cgtgcggtcg
     1141 cgtagcccga aactgacata gaatggcctc taggcgtttg ggcttgcgcg ccgctccgag
     1201 cgctgcgtag cgtcgcctcg gccgggttcg aaagatggat gatgcacgct cgcggttcgg
     1261 gccgattcgc cttcgatgca gtgcgctcgt ttgcgtcgcc gcatgcctca ccgcatgcgg
     1321 agccgacacc gaaaggagcg gttccgcctc gggggaccgc gcgaacgccg gtagcggcgg
     1381 cgcgaatggc gggaggctcg gcaacggtgg ttcaggtgga gggcgccccg gcactgcgtt
     1441 cgccgagtac gtgcccgaag gcccaccggt agctctcgac ggagtggtga acgcttcgga
     1501 cattgcctgg aagccaacca ccgacacctt cttcgtcctc gcggatcggg tgagcgagct
     1561 ctacgaatac gagccggatt tccgagcccg acggcgcacg atcgcgctcg tgaacccgcc
     1621 cgaggacgcc gaaggactga gctacgtgcg gtcgctcggc acatccgatc gcctcgtgct
     1681 cagcaccgag gcaccgacca acatggcttg ggtcttcgac ttggacgaga gcgcgaccac
     1741 gctcgacttc gcaacggcgg tgcgccaacg ctacgagccg atcggcgctt ccaaggttcc
     1801 gaatcgcggt ctcgaaggtg tcgcgctccg agcgaacgaa gacggcacgc agaattggct
     1861 gtacgtatgt gaggagggcg agccgcagcg cgaaccgatc cgcgtgctcc ggttcgcgta
     1921 cgacgaaggg gggcaggcgc tcgagacgta tcgagacggc tcgctgagcg tggaggagcc
     1981 gttcgtggcc tccgcgctgt tcgacagcgc ggtgggagac ctttccggcc tgcactacga
     2041 cggagcgacc ggcacgctgc tcatcacgtc acacctcggc agccgattgc tgcgcgtgga
     2101 tccggactcc gggcaggtct gggatcagat gttgctgacg cgttcgcccc aatacgaggg
     2161 tgtcaccctc gcttctggca atcggctcgt tgtcgtcagc gagcccaatt gggtggagat
     2221 ctttcgcggt cgctaaagta caccggtgac cgcggccgtg acctcggttt acgggcgcgg
     2281 cggccttcgt cagtgcggtg cacgagaccg ctcgaaactt gtgagcgttg cgtgctaaat
     2341 gcggggcgcg ctcctcgatg gggcgcctca ggaggtaggt tcatgcggct ttggcaaaag
     2401 gggctctcac tcaccggcgc actcgcgtgc cttgggctcg ttgcgtgcga gcagtcgccc
     2461 cacgcggcga gcgcaatcga ctcgaacccg acgaaggctc gagatcacgt catcggctgt
     2521 ttcaaagatg cctatcaggc gaagcagggc acatccccgg tcgtcaacaa caacgagcgg
     2581 tcagcggccg agcagctcct ctcacgttac aacgggacgt cgagtcgcgc gtgcgaggcc
     2641 atcaacgaag cgatcgggca caacgtttgc gggctgccta tgtcgctcgt ctcgatcgtt
     2701 cagtgcgatc aacgtcagcg cgaggcgaag gcgggacagg gcgtcgatcc gaacacgaag
     2761 gcgcaaatca ccgaccgcat caaagagaac tcgcgcaagg acatgtcgcc gaacggcatg
     2821 agcgggaact gagccccgca ccgggcgcgg gaagaggcga gaaccggtga gcccgggcgg
     2881 ctgacgccgg agctttgcgg agcgcgaagg ttgcgctccg cggctcccga attcagcgca
     2941 ggccagactt cgaggtgagg gcgtccgggc acgaaacctg tttgtagacg accttcgggt
     3001 tgtccgcggc ttggagccat tcgacccccc acttgcagcc ggcgagcagg ctcgattggt
     3061 tcgagccctt gaagaggttg ttacacgcgg tttcggtgca actcttgcgc gccgcccagg
     3121 cgttgttctg tttttggcaa gtgagctgaa tgccgccgta ttgttcgccg agcgcggagc
     3181 ccgagacgcc ccattgttgt gtgcatgcgt tgaattgccc aacgccgccg cccggtatca
     3241 tcaggtcgaa ctggccctgg gtgatgttac cgatgttgat cacctgaacg accatggtct
     3301 tgccgcacag cgccgcggcc ccggcgtcgt ttgcttggct attcgattgc ccggtgaact
     3361 gaagctcgta gcacagaccg cactgactcc cgttgaacgc ggcgtagccg taagccaacg
     3421 tgtcactcac ggcccaggga gccatatccc agcaggcgaa tccgccccca ccgctgcacg
     3481 cgctctccgt gctcgaagcg gtactctggt tttgttggtt acacgtcttt gccggctgct
     3541 ttccccccgt gttgttcggc cacgagcacg agggcttgca gcaatcccaa aaccgcgtcg
     3601 tgtacccgtt gcttccgcca gtggggggag tgcattgcgt ggcgccgccg gagccctgca
     3661 tcgagccgcc gctcgatgta ttcgagctac caccgttcgc actgccgcc
//
LOCUS       DASTKU010000913         5007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_624560, whole genome shotgun sequence.
ACCESSION   DASTKU010000913 DASTKU010000000
VERSION     DASTKU010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624560"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFQ61_32160"
     CDS             complement(<1..551)
                     /locus_tag="VFQ61_32160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9959203.1"
                     /translation="MKIVIFGLTISSSWGNGHATLWRGLCRGLAARAHQVVFFERDVP
                     YYRNARDLWELDSGRLVLYEDWTAVRARAAAELADADAAIVTSYCPDGSAATDLVLDA
                     RHACRVFYDMDTPVTLAALQRGERPDYLGPRGVREFDLVLSFTGGKALQALREQHGAK
                     RVAALYGHVDPTVHRPAASNESFR"
     gene            complement(605..1675)
                     /locus_tag="VFQ61_32165"
     CDS             complement(605..1675)
                     /locus_tag="VFQ61_32165"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9959204.1"
                     /translation="MTDLNLPRVGALGVGWIGASRLAALSQSGQAEIVGICDPNPEAC
                     EKARACAPSARIVQSYDELLALDLDAVVIATPSGLHAEQCLRAFDRGVAVFCQKPLTR
                     SSEEAEAVLRAAQSSDRLLRVDFCYRQTQALSKLREVVQSGSLGRVYAVDLVFHNAYG
                     PDKAWANDANLAGGGCLIDLGVHLVDAAFWVLGAGHFERAHAQLFKQGQPLLPGALDV
                     EDFALGELETESSVNLRLACSWRSSFGTHARIRAEVFGTQGSATFANVDGSFYDFSCD
                     LHRGAHSERLVSPPDAWGDRAIVAFASELRESPRYRATDELLLVSRAIDALYGRRTVW
                     ASSPVRRARADSATGSVELNSP"
     gene            complement(1707..2681)
                     /locus_tag="VFQ61_32170"
     CDS             complement(1707..2681)
                     /locus_tag="VFQ61_32170"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9959205.1"
                     /translation="MSVGIRSMRAARIVGPRRVEVQSCPVKDPGPSQVRFKVEGCGVC
                     ASNLGPWFGLPWTQYPFAPGQSGHEAWGVVEDVGKNVRTLRPGSRIAAVSYDSFAEYD
                     VVDESAAVELPEEFADKPFPGEPLGCAMNVFERSNVQEGDIVAIVGVGFLGGILTRLF
                     SNMGARVIAISRRHDSLALARQMGATELIPMENHGEIIQRVQDLTGGKFCDRVVEATG
                     KQWPLDLAAELTRTRGRLMIAGYHQDGPRQVNMQLWNWRGLDVVNAHERDTAVYIKGI
                     RSAIRAVASHELDPTPFYTHRYGLEQLGEALDATDARPSGFVKALVAP"
     gene            complement(2796..2942)
                     /locus_tag="VFQ61_32175"
     CDS             complement(2796..2942)
                     /locus_tag="VFQ61_32175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959206.1"
                     /translation="MIPAVSSIRRNWAAVDACAFERVLALFTSDDFRTFVALAIATTG
                     TRSA"
     gene            3240..4355
                     /locus_tag="VFQ61_32180"
     CDS             3240..4355
                     /locus_tag="VFQ61_32180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9959207.1"
                     /translation="MPTVLITGGAGFIGSHLTDELLAHEYQVRILDNLCPQVHGPEQQ
                     RPNYLDRRAELQVGDVRDPSAVRRALQDVDVVVHLAAAVGVGQSMYMIREYTEINNIG
                     TAVLLEELPKLGIKKLVVASSMSIYGEGRYRDDAGALYDSAERSHDDLKAGRWEPRSP
                     NGAALSPIPTPEDKKPALSSVYALSKYDQERLCLMIGRAHGIPTVALRFFNTYGTRQA
                     LSNPYTGVLAIFASRLLNGKSPMVFEDGQQRRDFVSVHDVARACRLAIESERAAYRAI
                     NVGSGRSVSVNDVARSLAEILEKPALAPEVTGKHRVGDIRHCFADVSLAKELLGFVAE
                     IPLTQGLVQLGQWLRAERPVDRTETARRELETRGLTL"
     gene            4363..>5007
                     /locus_tag="VFQ61_32185"
     CDS             4363..>5007
                     /locus_tag="VFQ61_32185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9959208.1"
                     /translation="MIKSNLRGGYALITGGAGFIGTNLVERLVRDGTEVVILDNFSRS
                     GVERNAQHLQSLPGGRVHIERDDCRNAKLVSELVSGATQIFHFAAQVAVTTSLVDPVL
                     DFEVNARGVLNVLDAARKLEQRPPLVFTSTNKVYGALPGIRLIKANSRYEPAETSVRN
                     RGVSEAQPLDFHSPYGCSKGAADQYVLDYARSFGLPTAVFRMSCIYGPHQCGNED"
BASE COUNT         1001 a         1547 c         1551 g          908 t
ORIGIN      
        1 cgaaaactct cgttcgaggc cgcggggcgg tgcaccgtcg gatcgacatg cccgtagagc
       61 gcggcaacgc gcttcgcgcc gtgttgttca cgcaaggctt gcagagcctt acctccggtg
      121 aagctcaaca ccaagtcgaa ctctcgaacc ccgcgtggcc cgagatagtc cggccgctcg
      181 ccgcgctgca gcgccgcgag cgtaacgggc gtatccatat cgtagaacac gcgacaagca
      241 tgcctcgcgt ccaacaccag atcggtcgcg gcggagccgt cagggcagta cgaagtaacg
      301 atggctgcgt cggcatccgc aagctcggcc gcggctcgtg ctctcaccgc ggtccaatcc
      361 tcgtacagca ccaagcgccc cgaatcgagc tcccataaat cgcgcgcgtt ccggtagtag
      421 gggacgtctc gctcgaagaa cacgacctga tgtgcgcgcg ccgccagacc tcgacagaga
      481 ccacgccaga gtgtggcgtg gccattaccc cacgaagagc taatcgtcag accgaagatg
      541 actatcttca cttgaaacag ccctcgcggc gcgacgcgcc aaagatatgt tcaggaattg
      601 gcattcacgg agaattcagc tcgacggagc cggtcgcgga atcagctcga gcgcgtcgaa
      661 ctgggctcga cgcccacacg gtacgtctcc cgtatagcgc gtcgatcgcg cgcgagacca
      721 gcagcagctc gtcggtcgcg cggtagcgcg gagattcgcg cagctcgctc gcaaaggcca
      781 cgattgcccg gtcaccccac gcgtcgggcg gtgacaccaa gcgttcggag tgcgcgccgc
      841 gatgcaggtc gcagctgaag tcgtaaaatg agccgtccac gttggcgaag gtcgccgagc
      901 cctgggtccc gaagacctcc gcgcggatcc gagcgtgcgt gccgaacgac gagcgccaag
      961 aacacgccag ccgtaggttc acgctcgact ccgtttccag ctcgccgagc gcgaaatcct
     1021 cgacgtctag cgctcccggt aagagcggct ggccctgctt gaagagctgc gcgtgagcac
     1081 gctcgaagtg gcccgcgccg agcacccaga aggccgcatc cacgaggtgc actccgagat
     1141 cgatcaagca ccctccgccc gccaggttcg cgtcattggc ccaagctttg tctgggccat
     1201 acgcgttgtg gaacacgaga tccacggcat agacgcgccc caagctgccc gactggacca
     1261 cctcgcgtaa cttgctcagc gcctgggttt gtcggtagca gaagtcgacg cgcaacaaac
     1321 gatccgagct ctgggcggcg cgcaataccg cttccgcctc ttcggaagag cgcgtgagcg
     1381 gcttctgaca gaacacggcc actccgcggt cgaaagccct taggcactgc tcggcgtgca
     1441 gcccgctcgg ggtcgcgatc acgacggcat ccagatcgag agcaagcagc tcgtcgtaac
     1501 tttggacgat tcgcgccgag ggcgcgcacg cgcgcgcctt ctcgcaagct tcggggttag
     1561 gatcgcagat cccgacaatc tcggcttgtc cactctggct cagcgcggcc agccgcgatg
     1621 ctccaatcca gccgactccc agcgctccga cgcgcggcaa attcagatca gtcatgaaac
     1681 taccttaggg tgcagcgcgt ccccgatcag ggggcgacca gagcctttac gaatccactg
     1741 gggcgggcat cggtggcgtc cagcgcttcc ccgagctgct ccaaaccata gcgatgggtg
     1801 tagaagggag tcgggtccaa ctcatgcgaa gccacggcgc ggatcgcaga gcgaatgcct
     1861 tttatgtaga ccgccgtgtc gcgttcgtgg gcgttgacga cgtctagccc ccgccagttc
     1921 cagagctgca tgttgacctg acgcgggccg tcctgatgat agccggcgat catgagccga
     1981 cctcgcgtac gcgtgagctc ggctgccaaa tcgagcggcc attgtttgcc ggttgcttcc
     2041 acgacccggt cacaaaactt cccgcccgtg agatcttgga cacgttggat gatctcgccg
     2101 tgattctcca tcgggatgag ctcggtcgcg cccatctgac gggctaacgc gagtgaatcg
     2161 tggcggcgcg agatggcgat cacgcgggcc cccatgttac tgaagagccg tgtcaggatg
     2221 ccgcccagaa agcccacgcc aacgatggca acgatgtcgc cctcctggac gttgctgcgc
     2281 tcgaagacgt tcattgcgca gcccagcggc tcgccgggga aaggtttgtc cgcgaactct
     2341 tcgggcagct ccacggcggc actctcgtcc actacgtcgt actcggcgaa cgagtcgtag
     2401 gacacggccg cgatgcggct tccgggtcgc agcgtgcgca cgttcttgcc gacatcctcg
     2461 accacgcccc aagcctcgtg tccggactgg cccggcgcga aggggtattg agtccatggt
     2521 aagccaaacc agggtcccaa gttcgaggcg cacacaccgc agccctcgac tttgaaacga
     2581 acttggctag gtccaggatc tttgaccgga caactctgca cctcgacccg acgcggacca
     2641 acgatgcgag ctgcacgcat gctgcggatg ccgacgctca tgcaaacgac ttctgaagcg
     2701 tgtgtatgtt ggtgtcaagc caaattgaag tcggctaaat cgctcgctaa atggcggtcc
     2761 gtcttggggc aattcgcgca cgaatcggca tggattcagg cgctgcgtgt accggtagtt
     2821 gcgattgcga gcgctacgaa ggttcggaaa tcatcactcg tgaacagcgc cagaacacgc
     2881 tcgaaggcgc acgcatccac tgctgcccaa tttcgccgaa tcgatgaaac agcaggaatc
     2941 atgatgtttg cgcgatatcg attcatgcgc aaaggggtga aatgactcag cttgacgcgc
     3001 ttgacgacgc ggcttcaaat gtaagaacct tcaacaaaga acaaaaagga actggggaac
     3061 tggccaacga aagcgaacgg ttggagatgc ctaatttgga cacatctagt ctcggtgatc
     3121 ctagccaaga cgatcctaga gtccgcacgc tgatcgaact cgcgccgagc ggacgcgagc
     3181 gaagcggaaa tgagccgagc ccccaaaatc atggtctcaa gaaatctggg aaatcacgga
     3241 tgccgacggt cctaatcacg ggcggcgcgg gattcattgg ctcgcacctg acggatgagc
     3301 tcttggctca tgaatatcaa gtgcggattc tggacaatct ctgcccccaa gtgcacggtc
     3361 cagagcagca acggccgaac tatctggatc gacgggccga gttgcaagtt ggcgacgtgc
     3421 gggatccgtc cgctgtccgt cgggctttgc aggacgtaga cgtcgtggtt cacctggcgg
     3481 cggcggtcgg ggtcgggcag agcatgtaca tgatccggga gtacaccgag atcaacaaca
     3541 tcggcactgc ggtgctgctc gaggaactcc cgaaattggg aatcaagaag ctggtcgtcg
     3601 cctcgagtat gagtatttat ggcgaagggc gatatcgtga cgacgcgggt gcgttgtatg
     3661 actcggccga gcgtagccat gacgacttga aggcgggacg ctgggagccg cgctccccga
     3721 acggagcggc gctgagcccc attcccacgc ccgaagacaa gaagccggcg ctatcgtcag
     3781 tgtacgctct ttccaagtat gatcaggagc gcctgtgctt gatgatcggg cgcgcccatg
     3841 gaattcccac cgtggcgctg cgcttcttca acacgtatgg gactcggcaa gcgctatcca
     3901 atccgtatac cggagtattg gcgattttcg cctctcggct gttgaacggt aagtcgccga
     3961 tggtgttcga ggatggtcag caacggcggg atttcgtcag cgtgcacgac gtcgctcggg
     4021 cctgccggct cgcaatcgag tcggagcgcg ccgcctatcg tgccatcaac gtgggtagcg
     4081 gccgttccgt gagtgtcaac gacgtcgccc gctcgttggc tgaaatcctg gagaagccgg
     4141 cactcgcgcc cgaagtcacg ggcaagcatc gcgtgggtga cattcgtcac tgcttcgccg
     4201 acgtgagctt ggcgaaggag ctattgggct tcgtcgccga aatcccactc acccaggggc
     4261 tcgttcaact cggccagtgg cttcgcgcgg agcgtcctgt cgatcgtacg gagacggcgc
     4321 ggcgtgaact cgagactcgc ggtctgacgt tgtgaggtca tcgtgatcaa atcgaatctt
     4381 cgtggtggtt acgctctcat cactggtggt gcgggtttca ttgggacgaa cttggtcgag
     4441 cgcctggtgc gtgacggcac ggaggtcgtc attctcgaca acttttcgcg aagcggggtc
     4501 gagcgcaatg ctcaacactt gcagagcttg ccgggcggcc gagtacatat cgagcgcgac
     4561 gattgtcgca acgccaagct cgtgtcggag ctggtgagcg gcgcaacgca gatattccac
     4621 ttcgcggcgc aggtcgcggt gactacgagt ctggtcgacc cggtgctcga cttcgaagtc
     4681 aatgcgcgcg gcgtgctcaa cgtactcgat gcggcccgga agctcgagca gcgcccaccc
     4741 ctggtcttca cctccaccaa caaggtttat ggggcgctgc ccgggattcg cttgatcaag
     4801 gccaattcgc ggtacgagcc ggcggagacc tcggtcagga atcggggtgt ctccgaagcg
     4861 cagccgctcg actttcacag cccgtatggc tgctccaagg gcgcagccga tcagtatgtg
     4921 ctcgactatg cgcggagctt cgggttgccc acggccgtgt tccgaatgag ctgcatctat
     4981 ggtccccacc aatgcggcaa cgaggac
//
LOCUS       DASTKU010000914        16690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_847591, whole genome shotgun sequence.
ACCESSION   DASTKU010000914 DASTKU010000000
VERSION     DASTKU010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16690
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847591"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VFQ61_32190"
     CDS             complement(<1..600)
                     /locus_tag="VFQ61_32190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959209.1"
                     /translation="MKRRFPAGLSGVAWSAGLAWLLLAGCNGKFYFDQHSIQSGGEPS
                     GGHTSGGDTGGASEFGGSGGHGGSGGSGGSGGEPASTCAERCAAAGLLCQASNLRCVE
                     CTGDTDCRKKGLARCSFEGRCVACFEDHDCSLFDSDDDELMCEPATHSCVRACYAEEK
                     SCHEYECENERRPCVTCASDSECVGSTAGPHCIGDHTRCG"
     gene            complement(606..1790)
                     /locus_tag="VFQ61_32195"
     CDS             complement(606..1790)
                     /locus_tag="VFQ61_32195"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9959210.1"
                     /translation="MEPLKDYARLAAALLTRRSAADLASQLERVQVDREAGVAAVANA
                     IAERARRRQRRRWLGIAAGFSAAASIALVAGFVGGRHGGRLERAVAAASCSASVCGAS
                     ALGVGTLPSGKSFMPGEHLVAAPSGSSSVQFGPITRLTLSPAGELEFREGGPVRRFGL
                     LRGSLQLVVEKLKPGERFLVHTPDAEVEVHGTTFEVSVSEPSAPEARAACLGSRTRVR
                     VQEGVVRVRAGASEVSLSAGQVWPAQPCEETATPLGSAREKHADVATLSGVREASSGA
                     SARRAHHSASASASSHAGSSPGESPRALRAASDLNRQNDLYASALAARRAGDTTQALS
                     RFERLLGEFPGGPLSESALVERMRLLKSMDTERGRVAARQYLAKFPHGFARAEAERSL
                     GE"
     gene            complement(1791..2495)
                     /locus_tag="VFQ61_32200"
     CDS             complement(1791..2495)
                     /locus_tag="VFQ61_32200"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9959211.1"
                     /translation="MTESARARGFRLIPGGADSSARSPSPEPDSRKAEVAALSDLQLL
                     QAVRDGNGAAASLFHDRMRPLVDRTLMRLLGGHDPDYDDLAQQALIELVMSVDRFRGE
                     CPLDAWASIIVARVVYKQIRRRRSERKVIALDAGESREIADRRPQTGLALRSALRRIE
                     RHLAAIEDNKAWAFVLHDVHGYDLEEISSIMGVSRAAAQSRLVRGRKLLHERLSRDPE
                     LRDVLDALAPTLGGRA"
     gene            complement(2569..3660)
                     /locus_tag="VFQ61_32205"
     CDS             complement(2569..3660)
                     /locus_tag="VFQ61_32205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959212.1"
                     /translation="MTGRSQSSRGTQELPLSAPVVIVIWTLASELSSPGTQALTGAAH
                     EALGPDAQIVLKGASPTADDQELATAGPATSVVHLTWDTGEHRRARIVCYLPKTGRWL
                     ERQITFSQADPEVERGRTLGFFLASMFLEAGVVPEPRNKREPARPDESESEQKKEQAS
                     AVSQSGPESTRSSASRPSSPEPAKVTVRTAETTPVSSRNTALTLAAVSAFPGTSTGFG
                     VWVGAERVLRSSNLWLGARGEMRLGSVSAAQALSMFGSLGLSARWVYAEVREDLSLAF
                     RVSGSAAVLSLIHLSSDDPEPDWESRLTPVFELTHPIVLHLAGGSSLLVEAGAEFIPG
                     TTEVFVKGSRAAVWPMWSGLGRVGFQAIF"
     gene            complement(3664..5229)
                     /locus_tag="VFQ61_32210"
     CDS             complement(3664..5229)
                     /locus_tag="VFQ61_32210"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET9959213.1"
                     /translation="MPGLGRLDTVNPMDESELLQLLERVNRGGPEKYHQKNTEQNKLF
                     ARDRIRALVDAGTFVEDAQLANAEAAELPADGVITGIARCHGRKIALMANDSTVKAGS
                     WGQRTVEKILRIQEIATRLRCPLLYLVDSAGARITDQVEMFPGRRGAGRIFYNQVQLS
                     GAVPQICLLFGPSAAGGAYIPAFCDVVVMVDGNASMYLGSPRMAEMVIGERVSLEEMG
                     GAKMHTSVSGCGDVLCKDERSAIEWAKDYLRYMPQNCDERPVTSEPRPARGGLEPLAS
                     LIVPDENKAFDMRRVIEHVVDEGSFLEVKERFAREIITGFARIEGRAVGIVANQPKHL
                     GGVLFVDSADKAARFIWLCDAFNVPLLFLADVPGFMIGTKVERQGIIRAGAKMIAAVS
                     EANVPKISVVVRKAYGAGLYAMCGPAFEPDACLALPTASIAVMGAQAAVNAVYYNRIQ
                     EVPAGPEREQLVTQLREQYRRDVDLQKLASELVVDAVLPFSDLRTDLAARFALYEDKQ
                     RVAPRKKHLVPPM"
     gene            complement(5258..6064)
                     /locus_tag="VFQ61_32215"
     CDS             complement(5258..6064)
                     /locus_tag="VFQ61_32215"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET9959214.1"
                     /translation="MPSGSLEGRNERAPGQEEHGEASNEGGGPAWLGVEVSLRAPDEP
                     GVLVRSVVPDSPAERAGILPYDLILTVKGVQLQRPAQLIANISQCTPGERVAITLLRG
                     GRERLLSATLEAQPDAEALLRKRYVESPAPNLVELKSVQGSLEPNLQGLRGKVVVLEF
                     WATWCGPCRLTAPLLSLWSDRHAAEGLAVLGVTADPVAAAAQGAREMGMSYSVFSDET
                     GSTTQAYRAFALPTLFVIDRRGRVRDVMVGYSSARLRQIDDLLGKLLAER"
     gene            6429..6794
                     /locus_tag="VFQ61_32220"
     CDS             6429..6794
                     /locus_tag="VFQ61_32220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959215.1"
                     /translation="MALRLWPRLNRSPIPSEHWFGNYLAEVRASPGAERSYANLVTDT
                     LFPDGTWLIESLREARSGRSGPILVLARSDGEWSYWIASRSGGLAPPRQVEACKGCHD
                     AAPAAPVFGLPPALLGARE"
     gene            7830..8462
                     /locus_tag="VFQ61_32225"
     CDS             7830..8462
                     /locus_tag="VFQ61_32225"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9959216.1"
                     /translation="MQPTNSSAVRVRRAEPRDLKAVLRLLKELDAFHVGLEPRMFRKF
                     TGPARSEPWLLERFTQPDEVCFVAQRDKNIVGFVWAKAQPAPPLPVFVPERHLLIADL
                     IVTEAQRRAGIGQKLLSRALAWGKRRGLTRVQLSAFTENRAACALYEKLGFRPLSLVL
                     QGDIEALQTGLSHAPGHLSSQRSSNLYPDDGTDRMAGRARPAISDPALEK"
     gene            8452..9282
                     /locus_tag="VFQ61_32230"
     CDS             8452..9282
                     /locus_tag="VFQ61_32230"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9959217.1"
                     /translation="MKSEGELLRELPNVDAAVVWSFSAPWYELSPRLKHVFTPAAGRE
                     QVSPDPSSRVKTHHGAFHGQIMAESLLAMLLFMNRRLGPAMIAQQQGRWERGLGDSSV
                     ALRGQTALLIGYGAIGRQCAELLQAVGMVVHGLKREVEHGQAGVQRLFGPRQLHEALS
                     LADHVACILPGDTGTTHLIDETALSALKTSAFLYNLGRGNAIDLDALVRALEQRRLAG
                     AFLDVLPEEPPSPRSPAFHTPNLFFTPHASAIRKDYLDLYFSELATELQKLSTGQILS
                     "
     gene            9319..10191
                     /locus_tag="VFQ61_32235"
     CDS             9319..10191
                     /locus_tag="VFQ61_32235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET9959218.1"
                     /translation="MDMERRLERGLDGLESVDFGSTGFAPSGFGSLALGSLALGSLAL
                     GTGELDSAPSRRVPSVSTDVLPALRGDYVIERRDAVEWLEAQGEQSLDLIITDPAYES
                     LEKHRAHGTTTRLKHSKSSSNDWFQIFPNQRFPALFKAAYRALKKDRHFYLFCDQETA
                     FIVKPIAEAAGFKFWKPLVWDKQKIGMGYHYRSRYEFILFFEKGKRKLANLAIPDVLE
                     CPRIVGGYPTEKPVRLSQILVAQSSVASEIVADPFLGSGSVGVAALNLGRRFRGCDIA
                     QQAIVHAQSRLITG"
     gene            complement(10249..11532)
                     /locus_tag="VFQ61_32240"
     CDS             complement(10249..11532)
                     /locus_tag="VFQ61_32240"
                     /inference="COORDINATES: protein motif:HMM:NF021350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-associated domain-containing protein"
                     /protein_id="HET9959219.1"
                     /translation="MKGPTELGWEALDRHFSITDRLTRGEVIELSAPDIKNVSQREPR
                     LMSKFDTRESRPTPLRNATILPVSNGAYVVLPGDGYFDTESAARKKHWPASGSVRRLC
                     TLPWASGPTSESQALDMALATGVVHDFLQDPAACLTIRGRLRSPEFELGFGRSETPIP
                     LKVSGVQIEVDSGLEGEAIHVIEAKLGTRTNFHIRQLYYPYRMWLALQQYKPVKTVFF
                     SWSNRCFSFRSFGFTRPEHYHSIALLDAADYYLDDPALLPPLSRIVGGAGKEALPTEV
                     PFPQADDVRRVIDVLDAVSNGTSNRTELSKYYEFNERQSDYYANAAAFLGYLSRSDAG
                     FGLTPLGSEFVSLPMHERHRRFIERMAMLPVFREVLESLCSNRALPESDWTAELIRRS
                     TGLSGKTPERRARTVLSWARWAEQVTAEAERARAG"
     gene            complement(11534..12364)
                     /locus_tag="VFQ61_32245"
     CDS             complement(11534..12364)
                     /locus_tag="VFQ61_32245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET9959220.1"
                     /translation="MSASQAPATPAPARPFLKWAGGKGQLFDQIARHVPENFAKYHEP
                     FLGGGAMFFGLRPANARLSDINEELVGCYVAIRDQVEKVIAALKPHTYDKEHFYEVRD
                     KDPRRLKDPARAARMIYLNKTGFNGLYRVNASGKFNVPFGRHTNPLICDEVNLRACSA
                     VLQTTELSTSDFERVLDEARSGDFVYFDPPYVPISDTAYFTAYVPGGFGWDAQKRLHA
                     VFEELERKNVMALLSNSDVPELRKLYAGYRVNKVTATRRINSDALGRGKMSEVVISNF
                     "
     gene            13239..14219
                     /locus_tag="VFQ61_32250"
     CDS             13239..14219
                     /locus_tag="VFQ61_32250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9959221.1"
                     /translation="MAKLTNLPKISVVVPTRNNREELMRDLAPSLFARAGASFVQDAG
                     LSEEESPWLETILVDQSRTPELEAALTEAGLLPNHRISYVWTPEQCGISRARNLGVSL
                     SRGEIVVFLDSDIRVSPDHVIAIARKFVEHPEIDALFGPVLADVATSEAHENGTIPVF
                     VPGEACIVRPGECADHPVMGANFAIRREVALRFPFDEFLGAGGDFPSFEEVDQLLRLH
                     REGHSYGIFMEPTVHHTGVRVGAEVKTWANDTERGRGAVHAKQLMRGELGCLRDGPEM
                     ILKLAWSNFKRVGKPQGITRLLPYYKGLVEGLTTYELDREQMVYRGRRWS"
     gene            14397..15713
                     /locus_tag="VFQ61_32255"
     CDS             14397..15713
                     /locus_tag="VFQ61_32255"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HET9959222.1"
                     /translation="MSTTNLVFASREEHLSWLATEAQLPQGFRVGSAALSFLPVEVPK
                     PSRMTLSLLALDRPTEAFAAMFTRNAVPGAPVRIGRERVKGQSIGAIVVNTKVSNVGA
                     PGGVQASERVAAATARLLGFEPQQVLPSSTGVIGWRLPVDEMIKALPAAISSLQPYSV
                     LPAAQGIMTTDLYPKLRSVAVGSGRVVGIAKGAGMIEPNLATMLVFLLTDLALPREVL
                     RDALSAAVDESFHSMTIDTDTSTSDSVVLLSSQRVPCPDEEAFRRGLTQVCQDLAEDI
                     VRNGEGVHHVVRVNVSGAPNAAFARGVGKAVVSSPLFQCAVCGNDPNVGRLAMAVGKF
                     VGSQASELDLSRCSMRVGGHLIFSELEFRLDPTMETKLIAHLKAAELYESAAPEAGVF
                     RPPVNYPPHERSVEIDIHLGAGQASASVVGSDRSHEYISENADYRS"
     gene            complement(15746..16351)
                     /locus_tag="VFQ61_32260"
     CDS             complement(15746..16351)
                     /locus_tag="VFQ61_32260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959223.1"
                     /translation="MANLEPPNQTESDAYFRVCSTCRKPIGFGERYYRCSVSTCNRNR
                     LALYFCSVPCWDAHVPEARHRDAWAEEARAPTRAEWQAERQAQAAREGDDARAAGAAP
                     NRDSNPAARGGDVPTADASRERKIVTLKSESDAPEEVLVVVSKLKAYVKARSGFNTSD
                     AVMEVLSSHLRRICDRAVERAQSEGRKTVLDRDVRAVLGRE"
     gene            complement(16424..>16690)
                     /locus_tag="VFQ61_32265"
     CDS             complement(16424..>16690)
                     /locus_tag="VFQ61_32265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959224.1"
                     /translation="TAELSRNRTMKPSELRRRVLALFDEHARFKHLLEHHDGAERSGF
                     MAALDRGASPDVRRSLLDRSLSEWHERRGLLYPVLERARALTQG"
BASE COUNT         3250 a         5381 c         5068 g         2991 t
ORIGIN      
        1 gccgcagcgc gtgtgatcac cgatgcaatg gggccccgcc gtggagccga cgcactcgct
       61 gtcactagcg caggtcacgc agggccgacg ctcgttctcg cattcgtatt cgtggcacga
      121 cttctcctcc gcataacagg cgcggacaca cgagtgagtc gcgggttcgc acatcaactc
      181 gtcatcatcg gagtcgaata gcgagcagtc gtggtcctcg aaacacgcca cgcaacgccc
      241 ctcgaagctg caccgcgcga ggcctttctt acggcagtcc gtgtcccccg tgcactccac
      301 gcaacgcaag ttgctggctt ggcagagcag cccggcggcg gcgcagcgct cggcacaggt
      361 cgatgcaggc tccccgccac tgccgccact gccgccactg ccgccatgcc cacccgatcc
      421 gccgaactcg gaagcaccac cggtatctcc gcccgaggtg tgcccgccgc tcggttctcc
      481 accggattga atcgagtgtt gatcgaagta gaacttacca ttgcacccag cgagcaacag
      541 ccaggcgagg ccagcgctcc acgcaacgcc gctcaggccg gcaggaaacc gccttttcac
      601 gtttgtcact cgcctaacga gcgctcggct tcggctcgag cgaaaccgtg cgggaactta
      661 gcgaggtact ggcgtgccgc gacgcgccct cgctcggtgt ccatgctctt gagcaggcgc
      721 attctctcga cgagcgcgct ctcactcagc ggccccccag gaaactcccc gagcaatctt
      781 tcgaatcgcg agagcgcttg tgtcgtgtct cccgcgcggc gcgctgccag cgcgctcgca
      841 tacagatcgt tctgccgatt caaatcggaa gcagctcgca aggcccgcgg cgattcccca
      901 ggcgacgaac ccgcgtgcga cgaagccgaa gcagacgcac tgtgatgtgc gcgacgggcc
      961 gacgctccac tggaagcctc gcgcacaccc gaaagtgtcg ctacatccgc gtgcttttct
     1021 cgcgcagagc cgagtggggt cgcggtctct tcgcacggct gcgcgggcca aacctggccg
     1081 gcgctcaagc tcacctccga ggcacccgcg cgcacccgca cgacaccttc ctgcacccga
     1141 accctagtac gagagcccag acacgctgct ctagcttctg gagcgctcgg ctcgctcacg
     1201 ctcacctcga aagtcgtgcc gtgcacttcc acctcggcat cgggtgtgtg caccagaaaa
     1261 cgctcaccgg gcttgagctt ctccaccacc agttgcagcg agcccctcag caagccgaag
     1321 cggcgaaccg ggcctccctc tcgaaactcc agctcacccg cgggagagag cgtaagccgc
     1381 gtgatcgggc caaactgcac cgaggatgac ccgctcggcg ccgccactag gtgttcaccg
     1441 ggcatgaaag acttgcccga cggcagagtt cccactccga gcgcgctagc tccacaaacg
     1501 gacgccgagc agctggccgc ggccaccgca cgttccagac gtccaccatg acgaccacca
     1561 acgaacccgg caacgagcgc gatcgaggcc gccgccgaaa atcccgctgc gataccgagc
     1621 caacgccggc gctgcctacg tcgagcgcgc tccgcgattg cgttcgccac cgcagcaaca
     1681 ccggcttcgc ggtccacctg aacccgctcg agctgcgagg ctaaatccgc ggcggagcgg
     1741 cgagtgagca gcgccgccgc gagccgggcg tagtccttga gaggctccat tcaagccctc
     1801 ccgcccaagg tgggagccag agcatccaag acgtcgcgca actccggatc gcgactgagc
     1861 cgctcgtgga gcagcttgcg gcccctcacc aggcggctct gcgcggcggc tcggctcacc
     1921 cccatgatgg agctgatttc ttccaggtca tagccgtgca catcgtgcag cacgaaggcc
     1981 cacgccttgt tgtcctcgat ggcggccaga tgcctttcga tccggcgcaa tgcgctacgt
     2041 aacgcgagcc ctgtctgcgg ccggcgatcc gcgatttcgc ggctctctcc ggcgtcgagc
     2101 gcaatcacct ttcgttcact gcgtcggcgt cggatctgct tgtacacgac ccgcgccacg
     2161 atgatcgaag cccaggcgtc cagcgggcac tcgccacgga atcgatcgac actcatcacc
     2221 agctcgatca gagcctgttg cgccaagtcg tcgtagtctg gatcatgacc gccgagtagc
     2281 cgcatcagcg tgcggtccac gagtggtcgc atgcgatcgt gaaagaggga ggccgccgcg
     2341 ccatttccgt cccgtacggc ttgcagcagc tgcaggtcgg acagggctgc tacttcagcc
     2401 tttcgagaat ccggttcggg cgaaggcgac cgcgcggacg aatcagcgcc ccctgggatc
     2461 aggcggaagc cgcgcgcgcg cgcactctcg gtcatcgtct tcctaatgtg ccacgagttc
     2521 cggacgggca ggcagcgatg cgcagttcgc ccgcaagcag caaagctttc aaaagatcgc
     2581 ctgaaatcct actctgccca gtccgctcca cattggccac acggccgcgc ggctgccctt
     2641 gacgaaaacc tccgtcgtcc ctgggatgaa ctcggccccc gcttcgacca gcagcgagga
     2701 gcctccagcc aaatgcagca cgatagggtg ggtgagctcg aagaccggcg tcaggcggct
     2761 ttcccaatcc ggctccggat cgtcggacga caggtgaatc aacgagagca ccgcggccga
     2821 cccgctaacg cggaacgcca aactgaggtc ctctcggact tccgcataca cccagcgcgc
     2881 gctcaggccg agcgagccaa acatcgacaa ggcttgtgca gcactcacgc tgccgagccg
     2941 catctcacct cgtgctccga gccagaggtt cgaactacgc aacacccgct cagctccaac
     3001 ccacacccca aagccagtcg aggtgccagg aaaggcactc accgccgcga gcgtcaacgc
     3061 ggtgttccgg gagctgactg gcgttgtctc cgcggtacgt accgtcactt tcgctggttc
     3121 cggagagctc gggcgcgaag cggaagagcg cgtcgactcc ggacctgatt ggcttacggc
     3181 actcgcttgc tccttcttct gttcggattc gctctcgtcg ggccgtgcgg gttcgcgctt
     3241 gtttctcggc tccggcacga ctcccgcttc gaggaacatg gaagccaaaa agaagcccaa
     3301 cgtgcgcccg cgttcgacct ccgggtccgc ctgagaaaac gtgatctgtc gttcgagcca
     3361 acgccccgtc ttgggaaggt agcaaacgat tcgggcacgc cggtgctctc ccgtatccca
     3421 ggtgaggtgc accaccgagg tggcggggcc ggccgtggcg agctcttgat cgtccgcggt
     3481 cggcgatgca cccttcaaaa cgatttgcgc gtcagggccc agtgcctcgt gtgcagcccc
     3541 ggtcaatgct tgggttccgg gcgatgacag ctccgacgcg agcgtccaaa ttacgatgac
     3601 gaccggggca ctcagcggca attcctgggt tcctctcgaa ctctgcgatc taccggtcac
     3661 gaactacatg ggtggcacca ggtgcttctt gcgcggggcc acgcgttgct tgtcctcgta
     3721 caacgcgaat cgggccgcca agtcagttcg taaatcggag aagggcaaca ccgcgtcgac
     3781 cacgagttca ctcgcgagct tctgcaagtc gacgtcccgc cggtactgtt ctcgcaactg
     3841 ggtcacgagc tgctcgcgct cgggcccggc cggcacttcc tgaatacgat tgtagtagac
     3901 cgcgttcacg gccgcttgcg cgcccatcac cgcaatggac gcggtaggca gggccaagca
     3961 ggcgtcgggt tcgaaagcgg gaccgcacat ggcatacaaa ccagcgccgt acgccttgcg
     4021 cacgacgacg ctgatcttcg ggacgttggc ctcgctcacc gcggcgatca tcttggcgcc
     4081 cgcgcgaatg atcccttggc gttcgacctt ggttccgatc atgaagccgg gcacatcggc
     4141 caagaacagc agcgggacat tgaacgcatc acacaaccag atgaagcggg cggctttgtc
     4201 cgcgctatcc acgaacaaga ccccccctag gtgtttgggc tggttcgcaa cgataccaac
     4261 cgctcgtccc tcgatccgcg caaagcccgt gatgatctcg cgcgcgaagc gctcctttac
     4321 ctcgagaaag ctgccttcat cgaccacgtg ttcgatcacg cgacgcatgt cgaaggcttt
     4381 attctcgtcc ggcacgatca acgacgcgag cggttcgagg ccgcctcgcg ccggccgggg
     4441 ttcactcgtg accggccgct cgtcgcaatt ttgcggcatg tagcgcaagt agtctttggc
     4501 ccactcgatc gcagagcgct cgtctttgca cagcacgtca ccgcaacccg agaccgacgt
     4561 atgcatcttg gccccgccca tctcctccag tgagacgcgc tcgccaatca ccatctcggc
     4621 catgcgcggc gagccaaggt acatactggc gttgccgtcc accatcacca ccacgtcgca
     4681 gaacgccgga atgtaagcgc cgcccgccgc agaaggaccg aacaacaagc aaatctgcgg
     4741 cacggccccg ctgagctgga cctggttgta gaagatccgc cccgcccctc gccgccccgg
     4801 gaacatttcc acctggtcgg tgatgcgcgc ccctgccgag tccaccagat agagcaaggg
     4861 gcagcgcaac cgtgttgcaa tctcttggat gcgcaggatc ttctcgaccg tgcgttgtcc
     4921 ccacgatccg gctttgacgg tcgagtcgtt cgccatcagc gcgatcttgc gaccatggca
     4981 gcgcgcaatg ccggtaatca ccccgtccgc aggcaactcg gcggcttcgg cgttggcgag
     5041 ctgagcgtcc tccacgaacg tgcccgcgtc caccaacgcc cggattctat cccgcgcgaa
     5101 gagtttgttc tgctcggtat tcttctgatg atacttctct gggccgcctc gattaacccg
     5161 ctccagaagc tgcaagagct cgctctcgtc cattgggttt acggtatcca gccgacccag
     5221 accgggcaag ccccgagcca aaaggggccc ctttggctca gcgctccgcg agcagcttgc
     5281 ccaacaaatc atcgatctgt cgcaagcgcg ccgaggagta gccaaccatc acatcgcgca
     5341 cgcggccgcg ccggtcgatc acgaacaaag tgggcaaggc gaaggctcgg taagcctgag
     5401 tcgtagagcc ggtctcgtcg gagaacacgc tgtaggacat gcccatctct cgggctccct
     5461 gcgctgccgc cgcgacgggg tcggcagtaa cacccaaaac ggccagccct tcggcggcgt
     5521 gccggtcgct ccacaagctc agcaggggcg ccgtcaagcg acacggtcca caccaagtcg
     5581 cccagaattc gagcaccacg accttgccgc gcaagccctg caagttgggt tcgaggctac
     5641 cctgcacgga cttgagctcg actaggttcg gagcgggaga ttccacatag cgcttccgta
     5701 gcagcgcctc tgcatcgggc tgtgcctcca acgttgcaga caacaggcgc tctcgcccgc
     5761 cccgcaagag cgttatggca accctttctc ccggcgtaca ttgagagata ttcgcgatca
     5821 gctgcgccgg acgctgaagt tgaaccccct tcaccgtcag aatcaggtca taaggcagta
     5881 tgcccgcgcg ctccgcaggg gaatccggca ccaccgatcg gaccagaaca ccgggctcgt
     5941 ccggtgcgcg caacgagact tcgacgccca accatgctgg ccccccccct tcgttcgaag
     6001 cttcgccgtg ctcctcttgc ccgggagctc gctcgttgcg tccctcgagc gaaccgctcg
     6061 gcacggcggg cggttgttca gtagccgtgc gggccatcgg aggttcgtcc aagttccgca
     6121 cgggcggtga cggaccacaa ccccaagccg aacccactgc gcaccaagcc agcaggaacg
     6181 agcgaacgat cgctgaacgc agtctcaccc aaactggcta ccatgacagg cccaactcgg
     6241 cactgcgtca ccgggccagc agaagattcg cgctaatagc gagcctcggc ggtacctgcc
     6301 tcgcagtggc gtgcgcgcct cgcacagctg cagagacacc gggagcgagc acggtcagcg
     6361 ataatgaacg agtcgcagtc caagagccgc cgtcggatcc cgccactgaa gccgcgtctg
     6421 acctctcggt ggctctacgc ctttggccac gcctgaatcg gagcccgatc ccttcggagc
     6481 actggttcgg gaactatctc gctgaagttc gcgccagccc gggcgccgaa cgttcgtacg
     6541 ccaacctagt taccgatacg ctctttccag acgggacttg gctcatagag agcctgagag
     6601 aggcccgcag cgggcgcagc ggacccatcc tggttttggc gcgctccgat ggcgagtggt
     6661 cctattggat tgcgagtcgt tcgggaggct tggccccgcc tcgccaagta gaagcatgca
     6721 aaggctgtca cgacgctgcg cctgcggccc ccgtgttcgg gcttccaccc gcgctgctcg
     6781 gcgcacggga atgaaccccg ccttgctggc gttgggttac aaagacggaa gccccgcgtg
     6841 agcggggctt ctaaaaaacc gagggtgccg gggcggagcc tcgtctgact ccgccctaaa
     6901 cgggcaacag ctctcgatga gcaacgagcg accgctgcag aaacagctct gcagccaagt
     6961 catcgcgaga atcgaagaaa gaaagccgct cgatcacgag caataagcca gcactactcg
     7021 agctcagcgc cactaagcga agacgcaaga gaataaagcg cggaatcgaa ggaagccgaa
     7081 gaccgaacta agccgcaaag aactacgtca aaccaggaaa ctaagcggcg agcggaaact
     7141 cagccatcac gagcgccaag ccaagagaag caatcgcgaa aaaggcgaac aggggactaa
     7201 acgaaagacc ctaactcaag cgacagttca agctgaccaa gtcaagcccc agaacctaag
     7261 gataagaagc aaagaagagg ccccaggaac tggagaaggc aacaggcaaa gcggagaagg
     7321 aagcaggcgc taaggaaagg aagcgcgagg aactaagaaa aaggcggagc taaggaaagc
     7381 cgaactaagt aaaggaagag ccgaatctgc tgaagataag agttccgccg agaagaaatt
     7441 gaaggaaagt aagaaagctc cggttacacc gggttagcga ccagccaata agcagaatgc
     7501 atgccactcg ctggttcctc gtgtcttcgc agaaaccctt cgtgatctct acgaatcccc
     7561 gagtcccact ttcacacacc gagtgcgcac catggtcggt ctgtcatgtt gcacaagcca
     7621 ccctgcaaac ttttcctaag cgtggggtag agccctccag ggctcagccc ggaaggcgca
     7681 gctggaatgc agggggtccg gcgcaaacct gaagctcccg attgctgact gcgcgccagg
     7741 caacgagacc gcgaagacga gcgggaatcc gacgctgagg caagcgagtc cgtcaaaaac
     7801 ctggcaagtt gggtgtctcc aatcgagtca tgcagcccac gaacagctcc gcggtgcggg
     7861 tccgacgtgc cgagccacgc gacctcaaag ccgtgcttcg actgctgaag gagctggatg
     7921 cattccatgt cggactcgag ccgcggatgt tccgcaagtt cactggacct gcccgctcgg
     7981 agccctggtt gctagaacgc ttcactcagc cggatgaagt ctgtttcgtg gctcagcgag
     8041 acaagaatat cgtcgggttc gtatgggcaa aagcccaacc cgctcccccg ctacccgtct
     8101 tcgtcccaga acggcacctg ttgattgccg atctgatcgt tactgaggcg cagcggcgcg
     8161 caggaatcgg acagaaactg ttgagtcgcg cattggcttg gggcaaacgc cgtggactga
     8221 cccgcgttca gctcagcgct ttcaccgaaa accgtgccgc ttgtgcgttg tacgagaagt
     8281 tagggtttcg tcccttgtcg ctcgtattgc agggggatat cgaagcccta cagacggggc
     8341 tcagccatgc gcctggccat ctatcttccc aacgtagttc gaacctttac cccgacgacg
     8401 gcacagatcg aatggctggc cgcgcgcgtc ccgcaatatc ggatcctgca cttgaaaagt
     8461 gagggcgagc tgctgcgaga gctgcccaac gttgacgcgg cggtggtttg gagtttctca
     8521 gcgccctggt acgaattatc gccgcggctc aagcacgtgt tcacgcccgc ggcgggtcgc
     8581 gagcaagtct cgcctgaccc aagttcgagg gtaaaaacac accacggtgc cttccacggg
     8641 caaatcatgg ccgaaagtct gctcgccatg ctgttgttca tgaaccggcg cttgggcccc
     8701 gcgatgatcg cgcaacagca aggccgttgg gaacgagggc tgggggattc cagtgtggcc
     8761 ttgcggggcc aaaccgcgct cctgatcggg tacggcgcca ttggtcggca gtgcgcggaa
     8821 ttactgcaag cggtcggcat ggtcgtgcac ggactgaagc gagaggtcga gcatgggcaa
     8881 gctggcgtgc agcgcctgtt cgggccacgc cagctgcatg aggctttgag cctggcagac
     8941 catgtggcgt gcatcctgcc aggggacacc ggcacgactc acctgatcga cgaaacagcc
     9001 ctcagcgcgc tgaagaccag cgcgtttctg tacaacttgg gccgtggcaa cgccatcgat
     9061 ctcgatgcct tggttcgagc tctggaacag cgtcgcctgg ccggcgcatt cttggatgtg
     9121 ttgcccgaag aaccgccctc gcctcgtagc ccggccttcc acacgccgaa cttgttcttc
     9181 actccgcacg cgtctgcgat ccgaaaggac tacttggatc tgtacttttc agagctcgca
     9241 accgagttgc aaaaactatc gaccggacag atcctgtcct agctaggctt gagccgtgga
     9301 gcgtcttctg gcaattggat ggacatggaa cggcgcctgg aaagaggtct ggatgggctc
     9361 gagagtgtgg actttggcag cacgggtttc gccccctcgg gctttggtag tttggccttg
     9421 ggcagtttgg ccttgggcag tttggccttg ggtacagggg agctcgatag cgcgccatcc
     9481 cggcgggtgc cgagcgtgag tacggacgtc ctacctgcgc tgcgcggtga ttacgtgatc
     9541 gagcgtcgcg acgccgtgga gtggctcgag gcccagggag aacaaagctt ggatctaatc
     9601 atcaccgatc cagcctacga atcactcgag aagcatcgcg ctcacggcac cacgacgcgg
     9661 ctgaagcaca gcaagtcctc cagcaacgat tggttccaga tttttccgaa ccaacgtttc
     9721 ccggcgctct tcaaggcggc ctatcgagca ctcaagaaag atcgtcactt ttacttgttt
     9781 tgcgaccagg agacggcgtt catcgtcaag cccatcgccg aagcggccgg cttcaagttc
     9841 tggaagccgc tcgtctggga caagcagaag attggtatgg gctaccacta ccgctcacgc
     9901 tacgagttca tccttttctt cgaaaagggc aaacgcaagc tcgcgaacct cgcgattccc
     9961 gacgtgctcg agtgcccgcg gatcgtgggc ggatatccga cggagaagcc cgtacgcttg
    10021 tcgcaaattc tggttgcgca aagctccgtg gcttccgaaa tcgtcgcgga cccgtttctt
    10081 ggctctgggt cggttggagt ggccgcgttg aacctgggcc ggcgttttcg cggttgtgac
    10141 atcgcgcagc aagccatcgt gcacgcccaa agccgcttga tcacgggctg acgttcctag
    10201 tgaccccgga gttccggccg ccatctcgcg ggtttggatt ggcgagaact agcctgcgcg
    10261 cgctcgctcg gcttcggccg taacctgctc cgcccagcgc gcccaagaga gcacggtgcg
    10321 agcgcgtcgc tcgggcgtct tgccggacaa tccggtgctg cgccggatca actccgcggt
    10381 ccagtccgat tcgggcaagg cacggttcga acagagcgac tccagcacct ctcggaaaac
    10441 cggcagcatc gccatgcgtt cgatgaagcg gcggtgccgc tcgtgcatcg gcaagctcac
    10501 gaattcggag ccgagcggcg tcaacccaaa gccggcgtcc gaacgtgaaa ggtagccgag
    10561 gaacgcggcc gcgttggcgt agtagtcgga ctgccgctcg ttgaactcgt agtacttgga
    10621 gagctcggtg cggttggagg tgccgttcga gacggcgtcg agcacgtcga ttacccgacg
    10681 cacgtcgtcg gcttgcggaa aaggcacttc cgtcggcagc gcttccttgc ccgccccgcc
    10741 cacgatccgc gagagaggcg gaagcagcgc cgggtcgtcc aagtagtagt ccgcggcatc
    10801 gagcagggcg atggaatgat aatgctcggg gcgcgtgaac ccgaagctgc ggaaagagaa
    10861 gcagcggttg ctccacgaaa aaaacacggt cttgacgggc ttgtactgct gcaacgcaag
    10921 ccacatccga tacggatagt agagctggcg aatgtgaaag ttggtgcggg tgcccagctt
    10981 ggcctcgatc acgtgtatgg cttccccctc cagtccagag tccacctcga tttgaacgcc
    11041 gctcactttc aatgggatcg gcgtttcgga gcgaccaaac ccgagctcga attcaggcga
    11101 acgcagccgg ccccgaatcg tcaggcacgc cgctgggtcc tgcaggaagt cgtgcacgac
    11161 gccggtggcg agggccatgt ccagcgcttg cgactcggag gtcggcccgg atgcccatgg
    11221 cagagtgcac aagcgcctta cggagccgga ggcgggccag tgcttcttgc gggccgcgct
    11281 ctcggtatcg aagtagccgt cgcccggcaa taccacgtag gcgccgttgc taacgggtaa
    11341 gatcgtggcg ttgcgcagcg gcgtgggtcg gctctcgcgc gtatcgaact tactcatcag
    11401 acgtggctcg cgctggctca cgttcttgat gtccggcgcg gatagctcaa tcacctcccc
    11461 gcgtgtcagc cgatccgtaa tcgagaagtg acggtcgagc gcctcccaac ccagttcggt
    11521 agggcccttc atgtcagaaa ttgctgatca cgacttccga cattttgccg cgtcccagcg
    11581 cgtccgagtt gatccggcgc gtcgcggtca ccttgtttac gcgatacccc gcgtacaact
    11641 ttcgcagctc gggcacgtcc gagttcgaga gcaacgccat cacgttcttg cgctccagct
    11701 cctcgaatac ggcgtgcagc cgcttctgcg catcccaacc aaacccaccg ggtacgtagg
    11761 cggtgaagta cgcggtatcc gagatgggca cgtacggcgg gtcgaagtaa acgaagtccc
    11821 ccgatcgcgc ttcatccagc acacgctcga agtcgctcgt cgaaagctcc gtggtctgca
    11881 gcacggccga acatgcacgg aggttgacct cgtcacagat cagcgggttc gtgtggcgac
    11941 cgaagggcac attgaacttg cccgaggcgt tgacacgata cagcccattg aagccggttt
    12001 tgttcaggta gatcatgcgc gcggcgcgtg caggatcttt gaggcgccgc gggtctttgt
    12061 ctcgcacctc gtaaaagtgc tctttgtcgt aggtgtgcgg cttgagagcg gcgatgacct
    12121 tctcgacctg atcgcgaatc gcaacgtagc agcccacgag ctcttcgttg atatcgctca
    12181 gccgcgcgtt tgcgggccgg agcccgaaga acatcgcccc accccccaaa aagggttcgt
    12241 gatacttggc gaagttttct ggaacatggc gtgcgatctg atcgaagagc tgccccttac
    12301 cgcccgccca cttcaaaaaa gggcgggcgg gtgccggagt ggcaggggct tgcgaggcag
    12361 acatcgggct ggcgttccta gcgcggaaac ccgccagaag tcgaggcgct ggtagcccgg
    12421 ctggtctatt ggcgaggaat tcgagctcac tcggagccgc atctcgcgac gtcaggtgga
    12481 agggccgcca cctggagtcc aaacgcgcac ctgactagcc actcagctcc attgccgggc
    12541 gagcagcggt tcgaatgcga acggctctgg gctgccaagg ggctgtcgca cgctcatacc
    12601 tgccgcctga cgctcctgac ggacgagtga ctcgatgcgg acatgtgaca gcaacggccc
    12661 agccgctgca cgcgggtgcc aactcgccga cgcactcgac tcgcttaatt ttcgcagaac
    12721 ggaaaccgat ttggaaccca gaagccgcag cctatcggag tgtccgattc agcccggaaa
    12781 acgcccgatt ttcgctggaa tctcaaactc cgagggtgaa gttgcaatcg tctcagcccg
    12841 ctactcgcaa gagctttccg tttcgcgccc cgcgcgaggc gcgaaacggt ttgcctggat
    12901 ctcgggtcgc gggaggcgag tcaaaacggc tgaaagccgc tagcccagtt cttgcgtggc
    12961 gaatgtcgcg tcacgatcga gcaactaatc tcctgccttt cttcagtaac agaatgaacg
    13021 cctcacgatg ggaaatgaga ggcattgtcc tcatcggggg cgttactagc ggcttgcaaa
    13081 ccccgcgcga gccgctacgt ccgagcaccc tcacgggttt gaacgaacac ccagcacgga
    13141 catcgactgt cggatcaggt ggctcgcagc catcactcgc actaggtttc gatccgtttg
    13201 agcggggaac gcgtatttct cggcgaaggg gtctgcacat ggcgaagcta actaacttac
    13261 cgaaaatcag cgtcgtagtt cccacgcgca acaaccgcga ggagctgatg cgcgatctcg
    13321 cgccgtcgct cttcgctcgg gctggagcca gcttcgttca agacgcaggt ctcagcgagg
    13381 aggaatcacc ctggctcgag accatcctgg tggaccagag ccgcacccct gagctcgaag
    13441 cagctttgac cgaggccggg ctcttgccca atcatcggat ttcttacgtt tggaccccag
    13501 agcagtgcgg catttcacgg gccagaaatt tgggtgtttc gctgtcacgc ggcgaaatcg
    13561 tcgtgttttt ggacagcgat attcgggttt cacccgacca cgtgatcgcc atcgcgcgca
    13621 aattcgtaga gcatccagag attgacgcat tgtttggtcc ggtgctcgcc gatgtcgcta
    13681 cttcggaggc ccatgagaac ggcacgattc ccgtattcgt gccgggcgaa gcgtgtatcg
    13741 tacggcctgg agaatgcgcg gatcatcccg tcatgggcgc gaacttcgcg atccgtcgcg
    13801 aggtcgcgct tcgcttcccc ttcgacgaat tcctgggggc cggcggcgat ttcccctctt
    13861 tcgaagaagt cgaccaactg ctgcgtttgc accgtgaagg tcacagctac gggatcttca
    13921 tggagcccac cgtgcatcac acgggagtgc gcgtcggagc agaggtgaag acttgggcca
    13981 atgacaccga gcgcgggcgc ggcgccgtgc acgccaaaca attgatgcga ggcgagctcg
    14041 gctgtttgcg cgacggtccc gaaatgatct tgaagctcgc atggtcgaac ttcaaacgag
    14101 tgggtaagcc gcaaggcatc acccgcttgt tgccctatta caagggtttg gtcgaaggtc
    14161 tgacgaccta tgaactagac cgcgagcaga tggtgtaccg aggccggcgc tggtcttgag
    14221 gaggtgccgg aagccgcgga gcttccggcg tttcctaggc tccatgcagg ttggagcggc
    14281 atccgcgtgg cggtgatggc ttctgtgcct agtgctctgt tcgtcagaag gggcggattg
    14341 cgcccgctgt tctcgagtcg cttgtcgggc tagccggttc aagttaagcc tagcgcgtgt
    14401 caaccacgaa cctggttttc gcatcgcgcg aagaacatct gagctggctc gcgacagaag
    14461 cgcagttgcc gcaaggtttc cgagtcggaa gcgctgcgct gtctttcttg cccgtggagg
    14521 taccgaagcc ctcccgcatg acgttgagtt tgctcgcgct cgatcgcccc actgaggcgt
    14581 tcgcggcgat gttcacgcgc aacgcggtcc ccggtgcgcc cgtgcgcatt ggacgcgagc
    14641 gcgtcaaagg ccagagcatt ggcgcgatcg tggttaacac gaaggtctcc aacgtgggcg
    14701 ctccaggcgg agttcaagcc tccgaacgcg tcgccgccgc taccgcccga ctgctcggct
    14761 tcgaacctca gcaggttcta ccgagctcga cgggagtgat cggctggcgc ttgcccgtgg
    14821 atgaaatgat caaggcgctg cccgcggcga tctcgtcgct ccaaccttac agcgtattgc
    14881 ctgccgcaca gggcatcatg acgacggacc tttatccgaa gctgcgcagc gtggccgtag
    14941 gctcgggtcg agtcgtcggg attgcgaagg gcgccgggat gattgagccc aatcttgcta
    15001 ccatgctggt gttcttgctg acggatttgg ccttgccgcg cgaggtgctg cgcgacgctc
    15061 tatccgcggc tgtggacgaa agctttcact cgatgaccat cgataccgac accagcacgt
    15121 ccgactcggt ggtgttgctg tccagtcagc gcgtgccctg ccctgatgaa gaggcattcc
    15181 gtcgggggct cacgcaggtg tgccaggact tggcggaaga tatcgtaagg aacggggaag
    15241 gcgttcacca cgtcgttcgc gtgaacgtca gcggcgcacc gaatgctgct ttcgcacgcg
    15301 gcgtgggcaa ggccgtcgtc agctcgccgc ttttccagtg tgccgtgtgc gggaatgacc
    15361 cgaacgtggg ccgcctggcc atggcggtgg gcaagttcgt gggcagccaa gccagtgagc
    15421 tcgacctttc acgttgttcg atgcgggtag gcgggcactt gatcttctca gagctcgagt
    15481 tccgactcga tccgaccatg gaaaccaagc tgatcgcgca tctcaaggcc gcagagctct
    15541 acgagagcgc ggcgccggag gcaggcgtgt tccgcccccc agtcaactac ccgcctcacg
    15601 agcgcagcgt ggaaatcgat attcacctcg gcgccggcca ggcctcggcc agcgtcgtag
    15661 gctcggaccg cagtcacgag tacatcagcg aaaacgccga ctaccgaagc tgacgccgtc
    15721 aatctcggcc ctgctgtcga ccctgctatt cgcgaccgag caccgcgcgc acatcgcggt
    15781 cgagcacggt cttgcgaccc tcggattgcg ctcgctctac ggcgcgatcg cagatgcggc
    15841 gcaagtgcga ggagagcacc tccatcaccg cgtcggaagt gttgaacccc gagcgggcct
    15901 tgacgtaagc cttgagcttc gagacgacga ccagaacctc ctccggcgcg tccgattcgc
    15961 tcttcagggt cacgattttg cgctcccgcg aggcgtcagc cgttggaaca tctccgcccc
    16021 gcgccgcggg gttcgagtct cggttgggag ctgcgccggc ggccctggca tcgtccccct
    16081 ctcgagcggc ttgcgcctgc cgctccgctt gccactctgc tcgcgtggga gcgcgcgctt
    16141 cttccgccca agcgtcgcgg tgtcgagctt cgggcacgtg agcgtcccag caggggacgc
    16201 tgcaaaaata gagagcgagc cggttgcgat tacaagtgga gacgctgcac cggtagtagc
    16261 gctcgccgaa accaatcggc ttgcgacaag tgctgcaaac gcggaagtag gcgtccgact
    16321 cggtttgatt cgggggctct aggttggcca tgcgcgggcc ttgagcctag agcagcaaac
    16381 gttccgaaac cagcggtgaa agcgtcttgc cgatgcgagg cgactagccc tgcgtcaggg
    16441 cccgagcgcg ctcgagaacc gggtacagga gaccacgacg ctcgtgccac tccgatagac
    16501 ttcgatcgag cagactgcgc ctcacgtccg gcgacgctcc gcggtccaac gcggccatga
    16561 accccgaacg ctcggcaccg tcgtggtgct cgagcagatg cttgaagcga gcgtgttcgt
    16621 cgaacaacgc caacacgcgc cgtcgcaatt cactgggttt catggtgcga ttgcggctca
    16681 gctcagccgt
//
LOCUS       DASTKU010000915         3298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_847659, whole genome shotgun sequence.
ACCESSION   DASTKU010000915 DASTKU010000000
VERSION     DASTKU010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847659"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VFQ61_32270"
     CDS             complement(<1..264)
                     /locus_tag="VFQ61_32270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959225.1"
                     /translation="MSDESTETIEGGSKKPEASEMAARNWRELIRPKAIHADGERGNE
                     FYGKFSCEPLERGFGITIGNSLRRVLLSSLQGAAITAVRIDGAL"
     gene            complement(469..1098)
                     /gene="rpsD"
                     /locus_tag="VFQ61_32275"
     CDS             complement(469..1098)
                     /gene="rpsD"
                     /locus_tag="VFQ61_32275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET9959226.1"
                     /translation="MARYIGPVCKLCRREGGKLYLKGQRCFSEKCSVTRRPYPPGQHG
                     QARQKISEYGLRLREKQKMRRIYGLLEHQFRLYYTEASRLTGRTGEEMIGLLERRLDN
                     LVHRAGFTLTRAQARQLVRHNHILVNGKRVNIPSYRVNVGDKVEIREKSRKIAYLTSS
                     LQTAATREGASWLDVDGANFAVTFKSLPPRDELNEPSMREQLVVEYYSR"
     gene            complement(1127..1537)
                     /gene="rpsK"
                     /locus_tag="VFQ61_32280"
     CDS             complement(1127..1537)
                     /gene="rpsK"
                     /locus_tag="VFQ61_32280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012397714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET9959227.1"
                     /translation="MAAATSSAGRRPRGGKKIVRKNVPTGIAHIQSTFNNTIVTITDI
                     TGNAIAWASAGARGFKGSRKSTPFAAQLAAEEAGRKAMEHGMRSVAVFVNGPGAGRES
                     ALRGLQSAGFRITLIRDVTPVPHNGCRPPKRRRV"
     gene            complement(1544..1909)
                     /gene="rpsM"
                     /locus_tag="VFQ61_32285"
     CDS             complement(1544..1909)
                     /gene="rpsM"
                     /locus_tag="VFQ61_32285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET9959228.1"
                     /translation="MARIAGVDLPRRKAIRFALRYVYGIGDKTAVAICAKANIPEARQ
                     VEELTDADVKAIREIIEREYKVEGDLRREVQQNIKRLMDLGCYRGLRHRRNLPVHGQR
                     TKTNARTRKGPKRGVTRKK"
     gene            complement(1940..2053)
                     /gene="rpmJ"
                     /locus_tag="VFQ61_32290"
     CDS             complement(1940..2053)
                     /gene="rpmJ"
                     /locus_tag="VFQ61_32290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET9959229.1"
                     /translation="MKVRPSVKKICEKCKVIRRKGVVRVICDNPRHKQRQG"
     gene            complement(2384..3001)
                     /locus_tag="VFQ61_32295"
     CDS             complement(2384..3001)
                     /locus_tag="VFQ61_32295"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET9959230.1"
                     /translation="MRIVLLGAPASGKGTQAAFLEARYGIPKISTGDMLRAARKAGTD
                     LGKEAERYMSAGQLVPDEVVIGLVAERLKDADAQKGFILDGFPRTVAQAEALEAALIA
                     AQSPLDCVLQIDVPRELLMERATGRRTDVRTGQIYHLKYNPPPPDAQVEHRADDQEET
                     VSKRLDAYEAVTAALLPHYTQKNLLKRVDGTGSPAEVTARIVAVL"
     gene            complement(3014..>3298)
                     /locus_tag="VFQ61_32300"
     CDS             complement(3014..>3298)
                     /locus_tag="VFQ61_32300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET9959231.1"
                     /translation="IPGIRPGRQTAEYIDRVVSRVTVGGAIYIAAICVVPSVVASAFR
                     IPFQFGGTSLMIVVGVALETVNQIEAHLITQSYEGLTGPRATRLSGRRPA"
BASE COUNT          580 a         1031 c          977 g          710 t
ORIGIN      
        1 caacgccccg tcgatgcgaa ccgccgtgat ggcagcaccc tgcaacgacg aaagcaacac
       61 gcgacgcagc gagttaccga tcgtgatacc gaacccacgc tccaacggct cgcagctgaa
      121 cttgccgtag aactcgttac cgcgctcgcc gtcagcgtga atggccttcg gacggatcag
      181 ctcgcgccaa ttgcgcgctg ccatctcgga tgcttcgggc ttcttggatc caccttcgat
      241 tgtttccgtg ctctcgtctg acatgattgc tcctgcggtt tgggttcgtg cgaccatccc
      301 agcagagggg agaaccaact ctcacatccc tgctcgtgat cagcccacgt tgttgtctac
      361 taacttcaag ggagcggaac cgagcgaccg cttgcggcca gccagaccca ctcccaacta
      421 aactttcact gtgcgtcgcg cccccgaagc aacgggagcg cgtcacgatt agcggctgta
      481 gtactcgacc acgagctgct cgcgcatcga cggctcgttg agttcgtcgc ggggcggcag
      541 gctcttgaac gttacggcga agttggcacc gtccacgtcg agccagctcg cgccttcgcg
      601 cgttgcggcg gtctggagcg aggaagtgag gtacgcgatc ttgcggctct tctcgcgaat
      661 ctccaccttg tcacccacgt tcacgcggta gctggggatg ttgacccgct tgccgttgac
      721 gaggatgtgg ttgtgacgca cgagctgacg agcctgagcg cgcgtcaacg tgaagccagc
      781 gcggtggacg aggttgtcca accgacgctc gagcaagcca atcatctctt caccggtgcg
      841 gcccgtcaga cgactagcct cggtgtaata gagacggaac tggtgctcca agaggccgta
      901 gatacgacgc atcttctgct tctcgcgcag acgcagaccg tactcggaga tcttctgacg
      961 agcctgcccg tgttgacctg gggggtacgg acgacgggtt accgagcact tctcagagaa
     1021 gcagcgctga cctttgaggt agagctttcc accttcgcga cggcaaagct tgcaaacggg
     1081 gccaatataa cgtgccatag tcttccttct ttatccttag cccagattac acgcggcggc
     1141 gcttcggcgg acggcacccg ttgtggggca ccggggtaac gtcgcggatc agcgtgatcc
     1201 ggaagccagc agactgcagc ccgcggagtg cgctttcacg ccctgcgccg ggaccattga
     1261 caaacaccgc gaccgaacgc atgccgtgct ccatcgcttt gcgacctgcc tcttcggcag
     1321 cgagctgcgc agcgaacggc gtgctcttgc gtgagccctt gaaaccgcgc gcaccggcgc
     1381 tcgcccatgc aatggcgttg cccgtgatat cggtgatcgt aacgatggtg ttgttgaacg
     1441 tggactggat gtgggcaatg cccgtcggaa cgttcttgcg aacgatcttc ttaccgccac
     1501 gcggccgccg gcctgccgat gatgttgctg ctgccatgat agattacttc tttcgcgtta
     1561 cgccgcgctt cggtcccttg cgcgtacgcg cattcgtctt ggttcgctgc ccgtgtaccg
     1621 gcaggttgcg gcggtgccgc agaccgcggt agcagccgag gtccatcaga cgcttgatgt
     1681 tctgctgtac ctcacggcga aggtcaccct ccaccttgta ctcacgctcg atgatctcgc
     1741 ggatggcttt cacgtccgcg tcggtcagct cttccacttg tcgagcttcc ggaatgttcg
     1801 ccttcgcgca aatagctaca gcggtcttgt ctccgatgcc gtagacataa cgcaacgcga
     1861 agcgaatcgc ctttcggcgg ggcaagtcaa cgccagcaat acgtgccatt ctctcgtttc
     1921 tccttcagac ggaaacagct cagccctgac gctgcttgtg acgagggttg tcgcagatga
     1981 cccggactac ccccttgcgg cggatgactt tgcacttttc gcagattttc ttaacgctag
     2041 gacgaacctt cattttcttc gcctcagagc tttctagtga tttggccccg ggtggggtcg
     2101 ttagctgaaa gcctcaactc gacgcggtct cccgcgatga gtcgaaccag tgtgtgtcgg
     2161 gaagcagtcg aaagacccac tcgaacttgc cggccgtcct cgagttgcac gcgatacatg
     2221 gcgcgcggaa gaacttcgag aatggttccg gttcgtgctg tgttttgggt cgtttggtct
     2281 gctcctttcg tgatcttgtt gttgtcgcat cgaggggccc cggagagtcc ctagaactcg
     2341 ttactgctgc tcacgcttgc cgtcggtgtt gcgctgccgt cccctagagg accgcgacga
     2401 ttcgcgcagt gacttccgcc gggctcccag ttccatccac tcgcttgagc agatttttct
     2461 gggtgtagtg cggcaacagt gccgcggtga ccgcctcgta ggcatccaac cgcttggata
     2521 ccgtttcctc ctggtcgtcg gcccggtgtt ctacctgggc gtcaggaggg ggcggattgt
     2581 acttcaaatg ataaatctgt ccagtccgaa cgtcagttcg tcgtccagtt gcccgttcca
     2641 tgagcagctc ccttggaacg tcgatttgaa ggacacagtc tagagggctc tgggcggcaa
     2701 tcagcgcggc ctcgagcgcc tcggcttggg cgaccgtgcg cgggaaaccg tcgaggatga
     2761 agcccttttg cgcgtcggcg tctttcaggc gctcggcgac caggccaatg actacctcgt
     2821 ccggcacgag ttgcccggcg ctcatgtagc gctctgcttc tttgcccaag tccgttccgg
     2881 ccttacgtgc cgcacgcagc atgtctccgg tagagatctt ggggatgccg tagcgagcct
     2941 ccaagaaagc cgcctgggtc cctttgccgc tcgcgggcgc accgagaagt acgattctca
     3001 taacgctccg gctttacgcc ggcctccgtc cgctcaagcg ggttgcacgc gggcccgtca
     3061 ggccttcgta gctttgggtg atcaagtggg cctcgatttg gttcaccgtc tcgagcgcca
     3121 caccgacgac aatcatcaag ctcgtcccac cgaactggaa cgggatgcgg aacgccgatg
     3181 cgaccaccga cggcacgacg cagatggcgg cgatgtagat ggcgccaccc acggtaaccc
     3241 gcgacacaac acggtcgatg tattcggcag tctgacggcc ggggcgaatg cccgggat
//
LOCUS       DASTKU010000916         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_401147, whole genome shotgun sequence.
ACCESSION   DASTKU010000916 DASTKU010000000
VERSION     DASTKU010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401147"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFQ61_32305"
     CDS             <1..522
                     /locus_tag="VFQ61_32305"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HET9959232.1"
                     /translation="LEQLFEGRGQLVVYHFMFAPDQERGCKSCSFWADSFDGIVAHLK
                     QRDTSFAAISRAPLAKLQTMAGQLGWSFKWVSSEGNAFNLEYGVYFAPEVLAADNRPY
                     NFGSSAAHGPDMPGVSVFVREGGQIFHTYSSYARGIDILNTAYNYLDLTPKGRNEGDR
                     PMSWVKYRDRYGE"
     gene            512..820
                     /locus_tag="VFQ61_32310"
     CDS             512..820
                     /locus_tag="VFQ61_32310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959233.1"
                     /translation="MASDCECSRAGGAEPINTRAGSWLTFAIGALVAFAPKCPLCIAA
                     YLSLLGLGMVSAKATGAMLAPLVWPLAALLLALGTWRLARSFRVWLHASSEPSPHERR
                     "
     gene            852..1838
                     /locus_tag="VFQ61_32315"
     CDS             852..1838
                     /locus_tag="VFQ61_32315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET9959234.1"
                     /translation="MPELKRGEAQFLRSCVQRFVESFESGVASVLRRELGGILPAPVR
                     ELQNELTVLTRQLDAKSRPVRVHPAHAPLIRRILVDARRSAAQNIEEPLQKVVDPDVN
                     KTLRRAVLPYQELLAASWVKAVAPARIPRITDFLSIHFAEQAAQQDTRLPRRLYDEKF
                     GILEAPSRALTDLAYYRRRCGVRELNLGFAYADIDDFKAVNSQFSEAVVDLRILPRFM
                     ETLESWAFARGHAYRFGGDEYVLLLPNADRALCLSLLRELTDRVAARELVRGVRLKLS
                     VGLCVVDPECFLTEREILLSANAAKQVAKAAGKQRIAVTCPPDHERVEVEPS"
     gene            complement(1871..>2635)
                     /locus_tag="VFQ61_32320"
     CDS             complement(1871..>2635)
                     /locus_tag="VFQ61_32320"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010333 - terpene synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0046246 - terpene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terpene synthase family protein"
                     /protein_id="HET9959235.1"
                     /translation="PVEVATFLRSIFGALDPASESSSTPTSEAASLFVPALRSIWSRV
                     APGSAPALRTRFLRHVANYIDGCVWEAENRRLAQVPSRAVFQAMRMFTSTLYQFWDFI
                     EYAGGFCLPDEVVEHPLLVELQRSANAVASYANDIFSLRKENHNRDFHNLVSVLQHEE
                     DLPLSAAYAKAIALHDDQVRHFCTLERLVPRFEGETGRNVAAYLEGMRIWMRANYDWS
                     IVTPRYAEIPDNDVGFAGASASMATGDRTSSHGPAA"
BASE COUNT          481 a          789 c          854 g          511 t
ORIGIN      
        1 ctcgagcagc tgttcgaggg ccgggggcag ctcgtggttt accacttcat gtttgcgccc
       61 gatcaggagc gaggctgcaa gagctgttcg ttctgggccg atagcttcga tggcatcgtc
      121 gctcacctga agcagcgcga cactagcttt gcggccatct cacgggcgcc gctcgccaag
      181 ctgcaaacta tggctgggca gctcggctgg agcttcaagt gggtatcctc cgaaggcaac
      241 gctttcaacc tcgagtatgg ggtgtacttc gcgccggagg tgctggcggc cgacaatcgc
      301 ccgtacaact tcggcagcag cgctgcccat gggcccgaca tgccgggcgt tagcgtcttc
      361 gtacgcgagg gcggccagat attccacacg tattccagct atgcgcgcgg gatcgatatc
      421 ttgaacaccg cttataacta cctcgatttg acaccgaagg gtcggaacga gggggatcgt
      481 ccgatgtcct gggtcaagta ccgggatcgc tatggcgagt gactgcgaat gctcgcgagc
      541 cgggggggcg gagccaatca acacccgtgc cggctcttgg ctgaccttcg cgatcggtgc
      601 gctagtcgcg ttcgcgccca aatgcccgct gtgtatcgcg gcatacctct ctctactcgg
      661 gttgggtatg gtgagtgcga aagcaacggg tgcgatgctc gcgccgctgg tatggccttt
      721 agccgcgctg ctgctggcat tgggtacatg gcggctcgcg cgctcgttcc gcgtttggct
      781 ccacgcatcg tccgagccgt caccgcacga gcgccgttga cgcggaaacc ggcatcctcg
      841 acgctctcgg gatgccggag ttgaagcgag gtgaagctca gttcttgagg agctgcgtgc
      901 agcggttcgt cgaatctttc gaatcagggg ttgcgagtgt gttgcgccgt gaactcgggg
      961 gcatcttgcc cgcacccgtg cgcgagttgc aaaacgagtt gacggtcttg actcgacagc
     1021 tggacgccaa gagccgccca gtgcgtgtgc acccggctca cgcacccctg attcgccgca
     1081 tcctggtcga cgcacgtcgc agcgccgcgc aaaacatcga ggaaccgctg cagaaagtcg
     1141 tcgatcccga cgtcaacaag accttacgtc gcgcggtgtt gccgtatcaa gagttgctgg
     1201 ccgcgagctg ggtcaaagcg gtggctcctg cgcgcatccc gcgaattacg gacttcctct
     1261 cgatccactt cgcggagcag gccgcccagc aagatacacg cttgccgcgc cgactgtatg
     1321 acgaaaagtt cggcattctc gaggccccga gccgcgcgct cacggatctc gcctactacc
     1381 ggcggcggtg cggagttcga gagctgaacc tcggctttgc ctatgcagac atcgacgact
     1441 tcaaagcggt gaacagccag ttctccgagg cggtggtgga cttgcgtatt ctgccgcgct
     1501 tcatggaaac tttggagagt tgggcgttcg cccgtggaca cgcctatcgg ttcggggggg
     1561 acgaatacgt gcttctgtta ccgaacgcgg atcgggcgct ctgcctttct ttgttgcgag
     1621 agctgaccga ccgcgttgcc gcgcgcgagc tcgtgcgcgg agtgcgcctc aagctgagtg
     1681 tcggactatg cgtggtcgat cccgagtgtt ttctcactga gcgagagatc ttgttgagtg
     1741 caaacgccgc caagcaagtc gccaaggcgg ccggcaagca gcgcattgcg gttacgtgcc
     1801 cacccgacca cgaacgagtg gaggtggagc cgagttgaga cggagtcgcg agccggctga
     1861 tgggtgagat tcacgcggct gggccgtggg acgaggtgcg atcgcccgtc gccatcgagg
     1921 cggaagcgcc cgcaaatccc acgtcgttgt ccgggatctc tgcgtatcgc ggcgtgacga
     1981 tggaccagtc gtagttggcg cgcatccaga tgcgcattcc ttctagatag gcagccacgt
     2041 tccgtccggt ctcgccctcg aaccgaggaa ccaggcgctc cagcgtgcag aagtggcgta
     2101 cctggtcgtc gtgcagcgca atggctttcg cgtaggcggc actcaggggc agatcctcct
     2161 cgtgctgcaa caccgagact agattgtgga agtcacgatt gtggttctcc ttgcgaagag
     2221 agaatatatc gtttgcgtag cttgctaccg cattggcgga gcgctggagt tcgaccaaca
     2281 gtgggtgttc gacgacctcg tcgggcaaac aaaaccctcc cgcatactcg atgaagtccc
     2341 agaactgata aagcgtcgag gtgaacatgc gcatggcctg gaacactgcg cggctcggaa
     2401 cctgcgccag acgccgattc tccgcctccc aaacgcagcc gtcgatgtag ttggcgacgt
     2461 ggcgtaagaa acgcgtgcgc agcgcgggag cgctgcccgg cgcaacgcga ctccagatgc
     2521 ttcggagcgc cggaacgaag agcgacgccg cttcgctcgt cggagtagac gagctttccg
     2581 aggccggatc gagcgccccg aagatcgatc ggagaaacgt cgcgacctct acggg
//
LOCUS       DASTKU010000917         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1072116, whole genome shotgun sequence.
ACCESSION   DASTKU010000917 DASTKU010000000
VERSION     DASTKU010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072116"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2052
                     /locus_tag="VFQ61_32325"
     CDS             <1..2052
                     /locus_tag="VFQ61_32325"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET9959236.1"
                     /translation="ALDSQRGMLLASTYDYPGRYKRWDMGFVNPPLVIEARQRQFRIA
                     ALNERGEQLLAELQATLAKLAAVESLERRGGDLHGTIAQPQGRFAEEERSKQPSVFSV
                     LRALVQSFFHPSERYLGLYGSFGYDLAFQFEPLKLRMDRPERQRDLVLYLPDEIVVVD
                     HQRATAVRYQYEFEAGGRATRGLARAGARVPFSGAATVEKACDTPPGGYAELVRVAHQ
                     SFKRGDLFEVVPGQTFFEPCAAAPSELFRRLRERNPSPYGFLANLGDAEYLVGASPEM
                     YVRVEDGRVETCPISGTIVRGDDAISDAEQILTLLSSKKDEAELTMCTDVDRNDKSRI
                     CEPGSVRVIGRRQIEMYSRLIHTVDHVEGRLRPEFDGLDAFLAHAWAVTVTGAPKAWA
                     MQFIEDHERSPRAWYGGAVGLIGFDGRLNTGLTLRTIRILDGVAQVRVGATLLYDSIP
                     EAEEEETHTKAAALLDAIRHPRPVVSRAAQRVDRVGDGKTVLLVDHQDSFVHTLANYI
                     RQTGARVVTLRAGFPEAELDSLRPDLVVLSPGPGTPTDFNVGGTLKSLVARGLPAFGV
                     CLGLQGLVEYCGGELGVLDYPMHGKSSVIRVTGGRLFEGLPTEFRAGRYHSLYALKSK
                     LPESLIASAESEDGVIMAVEHRSLPLSGVQFHPESILSLNGEIGHRILRNVLGSVCSR
                     A"
BASE COUNT          377 a          670 c          759 g          430 t
ORIGIN      
        1 gctctcgata gtcaacgcgg catgctgctc gcgtcgacct acgactatcc cggtcgttac
       61 aagcgttggg atatgggctt cgtgaacccg ccgctcgtga tcgaagcacg ccaacgccag
      121 ttccggattg ctgcgttgaa cgagcgcggc gagcagctgc ttgccgaact gcaagctacg
      181 ctcgcgaagc tcgcggcggt ggagagcttg gagcgccgag gtggcgattt gcacggcacc
      241 atcgcgcagc cccaggggcg cttcgcggag gaagagcgca gcaaacagcc gtcggtgttc
      301 tccgtgctcc gtgccttggt tcaatcgttc ttccatccgt ccgagcgcta cctcgggctc
      361 tacggctctt tcggctatga tctggccttt cagttcgaac cgttgaagct ccgcatggac
      421 aggccggagc gtcagcgtga tctcgtgttg tatctgcccg acgagatcgt ggtcgtcgac
      481 catcagcgag ctaccgcagt acgctaccag tatgaattcg aggcgggtgg gcgagctacc
      541 cgagggctag ctcgcgctgg cgcgcgggtc ccgttctcgg gcgcggcgac ggtcgaaaag
      601 gcctgcgata ctccgccagg cggctacgcg gagctcgtgc gggtcgcaca tcagtccttc
      661 aagcgcggag acctgttcga ggtggtgccc gggcagacgt tcttcgaacc ctgcgcggcc
      721 gcgccctcgg agctgttccg gcggcttcga gagcgaaacc cgtcgccgta cgggtttctc
      781 gcgaacctcg gagatgcaga gtatctcgtc ggggcgtccc cggagatgta cgtgcgggtc
      841 gaggacgggc gcgtcgagac ttgcccgata tccggcacga ttgtgcgggg tgacgacgca
      901 atcagtgacg ccgagcagat cctgactctg ctgagctcga agaaggacga agccgagctc
      961 acgatgtgca cggacgtcga tcgcaacgac aagtcgagga tttgcgagcc cggctcggtg
     1021 cgggtgatcg gtcgccggca gatcgagatg tactcgcgcc tgatccatac ggtggatcac
     1081 gtggaagggc ggcttcgccc cgagttcgac ggactggacg catttctagc gcacgcctgg
     1141 gccgtcacgg tgaccggcgc gccgaaggct tgggcgatgc agttcatcga agaccacgag
     1201 cgttccccgc gtgcatggta cggcggagcc gtgggcctga ttggcttcga cggtcgcttg
     1261 aataccggcc tcaccttgcg aacgattcga attctcgacg gcgtggcgca ggtacgagtt
     1321 ggcgccacgt tgctgtacga ctccattccc gaggcggaag aggaagagac gcacaccaaa
     1381 gcggcggcct tgctggacgc aattcgacat ccgcgtcccg tggtgtccag agcggcccag
     1441 cgggtcgatc gagtgggcga tggcaagacg gtgttgctgg tcgatcacca agactcgttc
     1501 gtgcacacgt tggcgaacta cattcgtcaa acgggtgctc gcgtggtgac gctgcgcgcg
     1561 ggctttccgg aagctgaact cgactcgcta cgaccggatc tcgtggtgct ctcaccgggc
     1621 cctggaaccc ctacggactt caacgtgggt gggaccctga agtcgctcgt agctcggggg
     1681 ctgcccgcat tcggggtttg cctcggccta caaggtctcg tggagtactg tggtggagag
     1741 ctcggagtgt tggattatcc aatgcacggc aagagctctg taatccgggt cacgggcggg
     1801 cgcttgttcg agggcttacc gacggagttc cgggcggggc gatatcattc gctgtacgcg
     1861 ctcaagtcga agttgccaga aagcttgatc gcgagcgcgg agagcgagga tggcgtgatc
     1921 atggccgtgg aacaccgcag cctgccgctc tccggcgtac agtttcatcc cgaatcgatt
     1981 ctgagtctga acggggagat cggtcaccgg attttgcgca atgttctggg gagtgtctgt
     2041 tccagagcct gatcgtgttt ttgggagggt ctgttccaga ccctcccccc gccgggagtg
     2101 aatcccggcg gggcccccct cccaaggcgc aagcgctgcg cgcggggccc cagccccgct
     2161 tgcggcgacg taatctgtcg gggtgaagct gaacgatgat gcgtctgccc ctgcgctgcg
     2221 cgcggggccc cagccc
//
LOCUS       DASTKU010000918         7260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1520609, whole genome shotgun sequence.
ACCESSION   DASTKU010000918 DASTKU010000000
VERSION     DASTKU010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7260
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520609"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..2121)
                     /locus_tag="VFQ61_32330"
     CDS             complement(130..2121)
                     /locus_tag="VFQ61_32330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9959237.1"
                     /translation="MSGVSFAAARSNWVLGFVVLGLACSSSSTSPPTGGSGGAAGSLN
                     PSAGNPASGATSGAPAAGAGATPGTTPGTTAQPSQGGAVNPGGGAPSAGSGGSVSMSG
                     APSTAGMPAGGGGGTGGTGGMPSTKPAPTLIGDVTFSTPSQAFKGELKVELATAVASA
                     EIRYTTDGTLPTATSQVYAGAITLTATTQLRAQAFVAGAPSGAPSTGLYIARTFDATS
                     DVPIVLMDSYGSGKPDKSTYKSVAVMVFEPVNGTASVASLPTIAARGGYHLRGQSSAG
                     FEQAPYKIEFWDNADKDLDYPVMGMPADSDWALIPPYYDRSLIRNPFTYTLGKEMGLE
                     APRTVNAEVYLNWTAGRPMDTADYQGIYWFSETIKNNKVRTNLKELKEDDNMLPQISG
                     GYIFKFDQLATEEPTLKCTGSNPISSGFGGPPRGGGMGGMTGAGMTGTCWTDLELVDP
                     DKATQPQLDWITQYIQTFHNSLHAMPIGDYAQYMDVPSFVDYFIVNELTRNVDSYVRS
                     AFYHKDRDGKIVAGPLWDYNFALAVGANESVNPTAGFMYNGNGSGGRNVNNWYPKLMA
                     DAAFKAQIKARWTALRQNLLSEASLDERITKLSTPMKNAVVRDYAKWSVDYIYKKGQG
                     SSFIKIAMPNGPTYDDQIQALRTYVKARVAYMDTEIAKF"
     gene            2359..4179
                     /locus_tag="VFQ61_32335"
     CDS             2359..4179
                     /locus_tag="VFQ61_32335"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET9959238.1"
                     /translation="MKHNVRSVVEATLASLKQRGLLEFPALPAFAVEAPKQAEHGDFS
                     CNVAMLLQKVSGKRGTEIADLIVNNLVDPDAVVKSVERAGPGFLNFRLADRVFQIVPR
                     EILAAGKGFGRQPANSTGKRALVEFVSANPTGPVHIGHARGAFMGDAIARLMDAAGYA
                     VTREFYINDFGKQVETLGRTVYKRYLQLFGQHVELAEGEYPADYVIEIAKSFRDEVGD
                     KYVAGPEKEAQRAAMLVGIRENLKTIRATLEKANIAHDVYFSEASLHESGEVLKLVEA
                     YRARGVTYEANVGRTREQKDKVRSEESKAAKYADQQKGGTFLMTATYQENGKYKLKDD
                     EDRVILRSDGSPVYLTADLAYHKQKFERGFDVMVNVFGADHSGHVPRIESGMALLGND
                     TSKLKFVLVQIVRITRGGEEVKVSKRKGTVFELVDLIEEAGADACRFLFLMKTRDAQI
                     DFDLDLIAHQSRENPVFYFQYGHARCAGILRKAREKGIEFTADAVTDAQLARLTLAEE
                     KALLKKMSQLPEVVTLAAERLEPHHVLYFCQDLIADFHSYYTQYKSDPIVSSDVEKSG
                     GRLALVAALKQTLQSAFNILGIDAPDHMELQEESDTAAEN"
     gene            complement(4211..5854)
                     /locus_tag="VFQ61_32340"
     CDS             complement(4211..5854)
                     /locus_tag="VFQ61_32340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9959239.1"
                     /translation="MEERAVTQYLETDDVADDDWGRTLPHEADDDLVGMTLNATYFVE
                     RILGEGGMGRVYLARHARILQKRVAIKVLHSEHNRNPEVVARFHREAEAAASISHPNV
                     LSVYDVDRTPQGRVYIVYEYLEGMELAEHLKNRKKLDVAQALHIIRQVCHGLAAAHSR
                     GVIHRDLKPQNIFLVGDFRDGVPARPHVKILDFGLSRFIEPLEGVELTKVGAVMGTPA
                     YMPPEQALGLRADQRADVYGVGAVLYKLLTGRPPFHEPSAQATVLAVVSSEPARPRAL
                     EPSIPEDLERVIECALSRDPANRYGDMWELERALSALEPENIQTVPSFTARAVLLPEP
                     PPAIIPANPSLPRIHLDSEPVEEKLERPRVLRGILLAGMWLAVILAVVVTGLEQVTRR
                     HLGRVELGFMLAVLFMLTMPLAVFWVARIRRRMWDHLQEVHDLLSDVRLALYATLMGC
                     GAAWVLLQLVDVVLLRLFRSPPIPRLEIAWLGWNLAFPAMGFVIAGWLVARRRMRRQM
                     RPGFLQGVSAGVLALVSVVLIAAIVTLSVHWRYGLPLPR"
     gene            complement(6001..>7260)
                     /locus_tag="VFQ61_32345"
     CDS             complement(6001..>7260)
                     /locus_tag="VFQ61_32345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959240.1"
                     /translation="TCAGLCRGECSGTMTQYYCDGPLLLPDCAVSSGCENLAAATAAI
                     HRVCAAGSAFVSGPTPPEVGPAIADNLPALLVSDARAAGLVRAAAHLEGAAAALTPLL
                     QSERARACTELALSRLSKSRAAIDFAVNAANSVAWQMTREPDPGAVCVANSFDDSCAS
                     CVKSTCCDALTACTQISGCFDAANCVRQCMSQGGTQASCASQCQLDTELFSTVESFIK
                     CQDTECQASCAGPIPNGGACVASGSTTYLVDDFEDQNTAAPNGNWHITNPDGFLGSIT
                     LNQPGALDSAFALGVAGSPDPGMSIDFNGCMDARGTSALSFQATSPDATTSTVMVTLR
                     IRTRPNELQSRGGACSGSQCGDYHLFTTTVPARAFGLKSVPWSWFLDSGAHPPDLSQI
                     LGIDFVLYGSQAQQIVIDDVKFEGPNL"
BASE COUNT         1415 a         2229 c         2289 g         1327 t
ORIGIN      
        1 tcgcgcgaga tctctggcct gcacgcgcac gccggtacaa ggccgggcga ggccgaacgg
       61 tcccagaaac ggaaaaggcg agcgtgagct cgccttttcg tcaccgacgc aatcgcggcg
      121 ctcggttctc tagaacttcg cgatctccgt atccatgtat gcgacgcgcg ccttgacgta
      181 agttcggagg gcctggattt gatcgtcgta cgtggggccg ttgggcatcg cgatcttgat
      241 gaagctgctg ccttggccct tcttgtagat gtagtcgacg gaccacttgg cgtagtcgcg
      301 cacgaccgcg tttttcatcg gggtggagag cttggtgatg cgctcgtcga gtgaggcctc
      361 cgagagcagg ttctgacgga gcgcggtcca acgtgccttg atctgcgctt tgaacgccgc
      421 gtccgccatc aacttgggat accaattgtt gacgttgcga ccaccagagc cgttgccgtt
      481 gtacatgaag ccggcagtgg ggttcaccga ctcattggcg ccgaccgcca gcgcgaagtt
      541 gtaatcccag agcgggcccg caacgatctt gccgtcgcga tccttgtgat agaaggcact
      601 gcgcacgtag gagtccacgt tgcgcgtgag ctcgtttacg atgaagtaat cgacgaagct
      661 cggcacatcc atgtactgcg cgtagtcgcc gattggcatc gcgtgcaacg agttatggaa
      721 cgtctggatg tattgagtaa tccagtcgag ttgaggctgt gtggccttgt cgggatccac
      781 gagctcgaga tcggtccagc acgtgccggt catgcctgcg ccggtcatcc cgcccatgcc
      841 accgccgcgc gggggcccac caaaacccga ggagatcggg ttcgagccag tgcacttcaa
      901 cgtcggctct tcggtcgcga gctgatcgaa tttgaagatg tagccaccgc tgatttgcgg
      961 cagcatgttg tcgtcttcct tgagctcttt caggttcgtg cggaccttgt tgttcttgat
     1021 ggtttcggag aaccaataga tgccctgata gtcggcggta tccatcgggc gccctgcggt
     1081 ccaattgaga tacacctcgg cgttcacggt acgcggcgcc tccaggccca tctctttacc
     1141 gagcgtgtac gtgaacggat ttcgaatcaa cgaacgatcg tagtacggcg gaatgagcgc
     1201 ccaatccgag tctgcgggca tgcccatcac cgggtaatcg agatctttgt cggcgttgtc
     1261 ccagaactcg atcttgtacg gcgcctgctc gaaacccgcc gacgattggc cacgcaagtg
     1321 atatccgccg cgcgcggcga tggtgggcaa tgaagcgacc gacgcggtgc cattgaccgg
     1381 ctcgaacacc atcaccgcga ccgacttgta cgtgctcttg tcaggcttgc cgctgccgta
     1441 gctatccatc agtacgatgg ggacgtcgga ggtcgcgtcg aacgtgcgcg cgatatagag
     1501 ccccgtgctg ggagcaccgc tcggcgcacc cgccacgaag gcttgtgcac gcagttgggt
     1561 cgtcgcggtc aaagtgatgg cgcccgcgta aacctgggag gtggcggtgg ggagagtgcc
     1621 atccgtcgtg taacggatct ccgcggaagc gaccgccgtc gcgagctcca ctttgagttc
     1681 gcccttgaag gcctgactcg gcgtcgagaa ggtcacatcg ccaatcagcg tgggtgccgg
     1741 tttggtagag ggcatgccgc ccgtacctcc ggtgccacca ccgccaccgg cgggcatgcc
     1801 cgcggtgctc ggagcacccg acatgctgac ggaacctccg cttccggcgg acggcgcgcc
     1861 acctccgggg ttgaccgcgc cgccttgaga cggctgtgct gttgtaccgg gcgttgtccc
     1921 gggcgttgcg cccgcacctg ccgcgggtgc gcccgacgtt gctcccgaag ccggattacc
     1981 tgcacttgga ttcagtgaac ccgcagcacc accgctgcct ccagtgggcg gggaggtcga
     2041 ggaagaggag cacgcgagcc ccaatacgac gaaccccaac acccagttcg acctcgcggc
     2101 cgcgaagctc actccactca tattgatgct ttctttccgg aagttaccgt cgagccgcgc
     2161 gtgcacaggg agccgcctcg aggtcgcgcc gtcaaagggt tgcttcctcg tcggatttgg
     2221 ggcagctccc gagccggagc gcgcgggcat agaagtttac tgactcacta gattcgcagc
     2281 tcaatcgtcg cgaacccgtc acccctatgt aactcatctc gtttgccgca aggcaacttg
     2341 aattagagac ctcagcagat gaagcacaac gttcgatccg tcgtcgaagc cacgctcgct
     2401 tcgctcaaac aacgcggact gctcgagttt cctgcgttgc ccgccttcgc cgtggaagct
     2461 ccgaagcaag ccgaacacgg tgacttcagc tgcaatgtcg cgatgctcct ccaaaaagtc
     2521 agcggcaagc gcggcaccga gatcgccgac ttgatcgtaa ataacttggt ggatcccgac
     2581 gccgtggtga agagcgtgga gcgcgcaggc cccggcttct tgaactttcg cttggcagac
     2641 cgcgtgttcc aaatcgtccc gcgcgagatt ctcgcggcgg gaaaggggtt tggtcgtcaa
     2701 cctgcgaact ccaccggcaa gcgcgcgctg gtcgaattcg tgtcggcgaa ccccaccggg
     2761 cccgtgcaca ttggccacgc gcgcggagcc ttcatgggcg acgccatcgc gcggctcatg
     2821 gacgccgccg gatacgccgt cactcgggag ttctacatca acgacttcgg caagcaggtc
     2881 gagactctag ggcgcaccgt ctacaagcgg tatctgcagt tgtttggcca gcacgtggag
     2941 ctggccgaag gggaataccc agctgactac gtgatcgaga tcgcaaaatc tttccgcgac
     3001 gaggtcgggg ataaatatgt cgccggcccc gaaaaagaag ctcaacgcgc cgcgatgttg
     3061 gtcggcattc gcgaaaatct gaagacgatc cgcgccacgc tggagaaagc aaacatcgcg
     3121 cacgacgtgt atttcagtga ggcctcgctc cacgagagcg gcgaggtgct gaagctggtc
     3181 gaggcgtacc gcgcccgcgg cgtgacgtat gaagccaacg tgggccgcac gcgggagcaa
     3241 aaagacaagg ttcgtagcga ggaatccaag gccgccaagt acgcggacca gcaaaagggc
     3301 ggcactttcc tgatgaccgc gacttatcag gagaatggca aatacaaact caaagacgat
     3361 gaagatcgcg tgattctgcg gtccgacggc agtccggtct atttaactgc cgatctggcg
     3421 tatcacaagc agaagttcga gcgcggcttc gacgtgatgg tgaatgtgtt cggggccgac
     3481 cactcgggtc acgtgccacg catcgagtcg ggcatggcgc tgctcggaaa cgacacctcc
     3541 aagctcaagt tcgtgttggt acagatcgtg cggattacgc gaggcggcga agaggtcaag
     3601 gtcagcaagc gtaaaggcac cgtcttcgag ctggtggatc tgatcgaaga agccggagcc
     3661 gacgcttgtc gtttcttgtt cttgatgaag acacgggacg cgcagatcga cttcgatctg
     3721 gatttgatcg cccaccagag ccgcgaaaac cccgtatttt acttccaata tgggcacgca
     3781 cgctgcgcgg gcattttgcg gaaggctcgt gagaaaggca tcgagttcac ggccgacgca
     3841 gtgaccgacg cccagctcgc tcgcctcact ttggcggaag agaaggctct actcaaaaaa
     3901 atgagccaat tgccggaggt cgtgacattg gccgccgagc gactcgagcc ccatcacgta
     3961 ctctatttct gccaagacct gattgccgac ttccacagct actatacgca gtacaagtcc
     4021 gacccaatcg tgagctcgga tgtcgagaag agtgggggcc gtctggccct ggtcgcggct
     4081 ttgaagcaga cgctacaaag cgcattcaat attctcggta tcgacgcccc cgatcatatg
     4141 gagttgcagg aagagtcgga tacggccgca gaaaattgag ccattccggc cattcgatgg
     4201 cggactcgga ttagcggggc agaggtaacc cgtaccgcca atgcaccgac agcgtcacga
     4261 tcgcggcgat cagcaccacg ctgacgagcg cgagcacccc cgcggagacg ccctggagaa
     4321 atccagggcg catctgccgg cgcattcggc ggcgcgccac cagccagccc gcgatcacga
     4381 agcccatggc cgggaaggct aggttccaac cgagccaagc aatctcgagt ctcggaatgg
     4441 gtggtgagcg aaacagccgg agcagcacca cgtctaccag ctgcagcaaa acccaggccg
     4501 ccccgcaacc catcagagtt gcgtagagcg caaggcgcac gtcggacagc agatcgtgga
     4561 cctcttgaag atggtcccac atgcgacgtc ggatgcgcgc gacccagaat accgccagcg
     4621 gcatcgtcag catgaagagc acggccagca tgaagccgag ctccactcga cccaagtgac
     4681 ggcgcgtgac ctgctcgaga ccggtcacga ccacggccag aatgaccgcc agccacatgc
     4741 ccgcgagcaa gatgccgcgc agcacccgcg gacgctcgag cttctcctct accggttccg
     4801 agtccaaatg aatgcgggga agcgatggat tggcgggaat gatggcggga ggcggctcag
     4861 gtagcagcac cgcacgcgcc gtaaacgagg gtacggtttg aatgttctcc ggctcgagcg
     4921 cggacagcgc acgttcgagc tcccacatgt cgccgtatcg attggcagga tcacgtgaca
     4981 gcgcgcattc gatcacccgc tccaaatcct cggggatgct cggttcgaga gcgcgaggcc
     5041 gcgccggttc gctgctcacg acggccaaga ccgtggcttg cgcagagggt tcgtggaacg
     5101 ggggccgtcc cgtgagcagt ttgtacagca cggcgccgac gccatatacg tccgcgcgct
     5161 gatcggcgcg cagccccaga gcctgctccg gaggcatgta tgcgggcgtg cccatcaccg
     5221 cgccgacctt ggtgagctcg accccttcga gcggctcgat gaagcgagac agcccgaaat
     5281 cgaggatctt gacgtgagga cgcgcgggca ctccgtctcg gaaatctccg accaaaaaga
     5341 tgttttgtgg cttcagatcc cgatgaatga ccccgcgcga gtgggccgcc gccaaaccat
     5401 ggcagacctg acggatgatg tgcaatgcct gagcgacgtc gagcttcttt cggttcttga
     5461 ggtgctcggc gagctccatc ccctccaggt actcgtacac gatgtacacc cgcccctggg
     5521 gcgtgcgatc gacgtcgtag acggataata cgttggggtg agagatactg gccgcggcct
     5581 cggcttctcg gtggaagcgc gcgaccacct ctggattgcg gttgtgctcg gagtgcagca
     5641 ccttgatcgc gacgcgcttt tggaggatgc gcgcgtgacg cgccaggtac acgcgaccca
     5701 tgccaccctc acccaagatt cgctcgacga aataggtggc gttgagggtc atgccgacga
     5761 ggtcgtcgtc ggcttcgtgt ggcaacgtgc ggccccagtc gtcgtcagca acgtcgtccg
     5821 tctcgaggta ctgggttaca gcccgttctt ccatgcgcgt gggcgcgtcg ctcccgagcg
     5881 atgcgccccg ctaggtttcc atgcggttag gcagccgtaa acccattcca cgacgtgtgg
     5941 gaggtttacc ccctttcccc actcgcattc ccgtgaccgc accacgggtc tgcgggtttc
     6001 ctacaggttt gggccctcga acttcacgtc atcgatcacg atttgttgag cctgcgagcc
     6061 gtagagcacg aaatctatgc cgagaatttg gctcaaatca ggcggatgtg ccccggagtc
     6121 caagaaccag gaccagggca cggacttcaa cccgaaggct cgcgccggca cggtcgtcgt
     6181 aaacagatgg tagtcaccgc actgggagcc gctgcaggca ccgccccgtg attgcagttc
     6241 gttcggccgc gtgcgaatgc gcagggtcac catcaccgtc gaggtggtgg catccggcga
     6301 ggtggcctga aaggaaagtg cgctggtgcc ccgcgcgtcc atgcaaccgt tgaagtcgat
     6361 ggacatacca ggatctgggc tgccagcgac gccgagggcg aaggcgctat cgagggcgcc
     6421 gggctgattc agagtgatcg agcccaggaa tccatctggg ttggtgatgt gccagttgcc
     6481 gttcggcgcc gcggtgttct gatcttcgaa gtcatctacc aggtacgtcg tcgagcccga
     6541 ggcaacgcat gcgccgccgt tcgggatcgg ccccgcacag ctggcctgac actcggtgtc
     6601 ttggcacttg atgaaggact cgacggtgga gaacagctcc gtatcgagct ggcactggct
     6661 cgcgcacgag gcttgggtgc ccccctggct catgcactgt cggacgcagt tcgccgcgtc
     6721 gaagcaaccc gagatctggg tgcaggccgt gagcgcgtcg cagcacgtcg atttgacgca
     6781 gctcgcgcag gagtcatcga agctgttggc cacgcaaacc gcgccgggat cgggctcccg
     6841 cgtcatctgc catgcgactg agttcgccgc gttgaccgca aagtcgatcg ctgcacgcga
     6901 tttggacaga cgggacaatg cgagctccgt gcaggctctc gctcgttcgg attgtaggag
     6961 aggagtcagc gccgccgcgg cgccttcgag gtgcgctgcg gcacgcacca gccccgcagc
     7021 gcgggcgtcg ctcacgagga gcgcgggcaa gttgtccgcg atggcgggcc ccacctcggg
     7081 tggtgtgggc ccgctcacga acgcgctgcc cgccgcgcaa actcggtgga tagccgccgt
     7141 agccgcagcc aggttctcgc agccggaaga gaccgcgcag tccggcaaga ggagcggccc
     7201 gtcgcaatag tactgggtca tcgtgccgct gcactcgccg cgacacaggc ctgcacaagt
//
LOCUS       DASTKU010000919         4677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1072209, whole genome shotgun sequence.
ACCESSION   DASTKU010000919 DASTKU010000000
VERSION     DASTKU010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072209"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1717
                     /locus_tag="VFQ61_32350"
     CDS             155..1717
                     /locus_tag="VFQ61_32350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959241.1"
                     /translation="MALGLVAWAGGNGCSQLESPHQSFGAAGGAGASSAPSQAAGTSG
                     LTGGAGGAAGCWDPRGFDGVGCGTCAASDIVAFENACSEISCTPFPNGMRLSKLLPNG
                     ELPSLPDRPKASASDKEPAQNQPAKAPAGFACDDLGAQGRLVYVTGSSAARGYLEQIA
                     QQLASRGVYLVYAATGSCSGVDAILNGTRTAADSGPATYWESASSSGKACDLPTSGAA
                     SDLAISDVFAQTCPGFELASLDDQGVRDVRGPIQTMIFAVPSNSRHSELSRQAAYFVF
                     GFGKDGGVRDASGAPAWDDESLLMPRAPGSGTQALVAAAIGVPADRFVGPAYASSEEV
                     AVALQEAGTDMDRAQRALGILAADHIESRNLRARIRALAFQDTRQGCAVYPDSTQASR
                     DKRNVRDGHYPLWGPLHFLYRVDTRGNPIDAQKREVMLDIIGYLSGTKPLPSGLNLLD
                     VYAENGLIPQCAMQVTRLQDGGNVQAFRSNTPCACSFEFKVTGSTACKSCASQGDCGA
                     AETCSFGYCERG"
     gene            2161..3381
                     /locus_tag="VFQ61_32355"
     CDS             2161..3381
                     /locus_tag="VFQ61_32355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9959242.1"
                     /translation="MSSLRFGSLDRYEPLCPIGRGGMAEVYLAAWEVAPHVYRPVVIK
                     RLLPHLSGDPAVAQMFLDEARLSCRLDHPRIVRTLQAGSIGGHCCIVMEYLDGQPLHR
                     FLKRAYEYDALPIAMAVHIAIAMLDALAYAHELRDYQGNSLDVVHRDVSPHNVFVTRS
                     GQIKVLDFGIAKARGQEGYTATGLIKGKVGYIAPEQALGAEVDRRADLFAVGVVLWEA
                     LTGMRLFKGGNEIATLNLNLHAPIPTPSSMRADVPAQLDDILVRALARDPRARFQAAE
                     EMKAELEGWASEAGALVEDDQIARWMEELFAFELREDQQRVLDAMRRHESVPPSAAHV
                     GVSRSSAPPSLSSRRGFWQRVSSRWTALGLERGLGRLVPNRFRNSESGQAWYSRIKLL
                     ALGVGFLGLGVMGR"
     gene            4053..>4677
                     /locus_tag="VFQ61_32360"
     CDS             4053..>4677
                     /locus_tag="VFQ61_32360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959243.1"
                     /translation="MISNHALVFKRHSCKRHWLLFACGVVGGLFTAPGAAHAEDTAPA
                     AAALSDATKAYEEVDYARAYELGRVALNRGQASVGDSVRLYTLLGISAAALGKTDEAR
                     RYFITALAIDPARELEQSLSPRVRAPYLEAKGTLSLYKEPIRLLARMQRSGTYLVVRL
                     EDPLALIKSLAVQTRNASTTAAPADEPEVELPAQPENRVLIASTLAGV"
BASE COUNT          825 a         1387 c         1565 g          900 t
ORIGIN      
        1 aactcgatgt ttggagctag gagtgacaat tgcgtgtcac taaaccataa tccttcgggc
       61 agaactgcga ctctgctaca cacgtcgcaa gttgtcttgc gtctcgagtc tggttacgaa
      121 tcgaacgaat gccactcttc gagggtggct tcggatggca ctcggcctag tggcttgggc
      181 gggcggtaac ggttgctcgc agctcgagag tcctcaccag tccttcggag ccgcgggtgg
      241 tgccggggcc agctctgcgc cttcgcaagc cgccggaact tcggggctga ctggcggagc
      301 gggcggcgcg gcgggctgtt gggacccacg cggcttcgac ggggtagggt gcgggacctg
      361 tgccgcttcg gatatcgtgg ctttcgaaaa cgcgtgttcc gagatttcgt gcacgccatt
      421 tccgaacggg atgcggttga gcaagctgct cccgaatggt gagttgccgt cgctgccgga
      481 tcgaccgaag gcctcggcct ccgacaagga gccggcgcaa aatcagcctg cgaaggcgcc
      541 agccgggttt gcgtgcgatg acttgggcgc acagggcagg ctggtttatg tcacgggctc
      601 gtctgccgcc cgcggatatc tcgagcagat cgcgcagcag ctcgcgagcc ggggggttta
      661 cctggtttac gcggccaccg gctcttgctc tggagtggac gccattctca acggcacacg
      721 aaccgccgca gattcggggc ccgcgaccta ctgggagagt gcctccagca gcggcaaggc
      781 ctgtgacttg ccaacgagtg gcgcggcgag cgacctcgcg atctcggatg tgtttgcgca
      841 aacctgccca ggtttcgagc tggcgagtct ggacgaccag ggagtgcgtg acgtgcgcgg
      901 gccaattcag accatgatct tcgcggtgcc gagcaatagc cgacactcgg agttgagccg
      961 gcaagccgca tatttcgtgt ttggattcgg aaaggacgga ggagtgcgcg acgcgagcgg
     1021 agcgcccgct tgggatgacg agagcctatt gatgccccgt gcgcccgggt cgggaacgca
     1081 agccttggtc gcggctgcaa tcggtgtgcc ggcggatcgg ttcgtggggc ccgcttacgc
     1141 ctcttcggaa gaggtcgcgg ttgcgctgca ggaggcgggt actgacatgg accgggctca
     1201 acgcgcactc gggatcctag ccgcggacca catcgagagt cgcaacttgc gcgcgcgtat
     1261 ccgcgcgctc gccttccagg acactcgaca aggttgcgct gtttacccgg actcgaccca
     1321 ggcatcgcgc gataaacgca atgttcggga tgggcactat ccactctggg gaccacttca
     1381 ttttttgtac agggtggata ctaggggcaa ccccatcgac gcccaaaaac gagaggtgat
     1441 gctcgatatc attggctacc tttccgggac gaaaccacta cccagcggcc tgaatctact
     1501 cgatgtgtac gccgagaacg gtttgatccc gcaatgcgcc atgcaggtca ctcgccttca
     1561 ggatggggga aatgtgcaag cgtttcggtc gaacacaccg tgcgcgtgtt cattcgagtt
     1621 caaggtgacc ggcagtacag cgtgtaaatc ttgtgcctcg cagggtgatt gcggagctgc
     1681 ggagacgtgt tctttcgggt attgcgagcg gggttgattg gctagctaac caagcggggt
     1741 gtgtatcgaa cgcggcactc ggggtggcga ctgcgatacg cgcagacttc ggaaaaacgg
     1801 cacttcatga cactgaaatg tcaccgtgta taacgagggc ggtgctagta aacgcattct
     1861 gctgggtgcc gaaaaactca actggtcgcg gcggccgcgc taactctgcg gccgtgctaa
     1921 ctgcggccgt gcgacaaagg gctcgagcag gctttgaatg acatgaccct ccgcaatagt
     1981 gcgttcgttg cgagtgcgac tcccggttct cttcgcgcgc ccagctcgag tggacccagc
     2041 tcgagtggac ccagctcgat ggccagaacc agctcgttag ctccgtcgaa ctcgttgtcc
     2101 acgtcgagcg cgctacctac gccgagcgta gatagcgggc agcgaccgag ctttttggca
     2161 gtgagtagcc tgcgtttcgg gtcgctcgat cgttacgagc cgttgtgccc gattgggcga
     2221 gggggcatgg ccgaggtgta cttggccgca tgggaggtcg cgcctcacgt ctatcgcccg
     2281 gtcgtgatca agcgcttgct gccgcacttg agcggggatc ctgcggtcgc gcagatgttt
     2341 ctggatgaag cgcggctctc ctgccgtctc gatcatccgc gcatcgtgcg cacgctgcag
     2401 gcgggctcca tcggcggaca ctgctgcatc gtcatggagt acctggatgg tcagcctctt
     2461 caccgcttct tgaagcgcgc ctatgaatac gacgcgttgc cgatcgcgat ggcggtgcac
     2521 atcgcgatcg cgatgctcga cgcgctggcc tacgcccacg agttgcgaga ttaccagggt
     2581 aactcgctcg atgtggtgca ccgcgatgtc tctcctcaca acgtgttcgt aacgcgcagc
     2641 gggcaaatca aagttttaga cttcgggatc gccaaggcga gaggccagga aggttacacg
     2701 gccactggcc tcatcaaagg caaggtcggc tacatcgcgc ccgagcaagc gttgggcgcg
     2761 gaggtggacc ggcgcgcgga tctgtttgca gtcggcgtgg tgctttggga ggccctgacc
     2821 ggtatgcggc tgttcaaggg cggtaacgag attgccactt tgaatttgaa cctgcacgcg
     2881 cccattccca ccccgtccag catgcgagcg gacgtgccgg cgcagctgga cgacattttg
     2941 gtgcgcgcgc tggctcgcga tcccagagct cgatttcaag ccgcagaaga gatgaaagcc
     3001 gagctcgagg gctgggccag cgaggcgggg gcactggtcg aggatgatca gatcgcgcgc
     3061 tggatggagg agctgttcgc gttcgagttg cgtgaagacc agcagcgcgt gctcgacgcc
     3121 atgcggcgcc acgagagcgt gcctcccagt gccgcgcacg tcggggtgtc tcgaagcagc
     3181 gctccaccgt ccctctcgag ccggcggggc ttttggcagc gcgtgagttc gcgctggacc
     3241 gcgttgggac tcgagcgggg gctcgggcgc ctggtcccaa atcggttccg gaactccgag
     3301 agcggacagg cttggtattc tcgtatcaag ttgttggcct tgggggttgg cttcctcggc
     3361 ttgggtgtga tggggcgctg attcaccatg cgtggcgtga gccgaagttg caactcgcca
     3421 gtccctcgcg gccggctgcg gttcatgaga acgcccgggg cgagcgaagc ggatcgggaa
     3481 ggcaaggcga ctccgacacg ctagagccaa gcacccgagc cacgtccaac acggaggcgg
     3541 gcggaacacc gcgcgatgcc gaacggcgcg agttctccgc gccaacgacc accgtttcgg
     3601 cgttggtgca ctcgagcgcg ggcaacggaa cccgtcgcga atggcgcgca atcgatcgcc
     3661 gacagctttc ctcgtccgct gctgggcgcg cgcctgttcg gcgggcagaa cccgcggccg
     3721 ccgcggcccc gggttctgat tcgagcctag cacccggcgc agctgagcct tcgagtggaa
     3781 acggtgcgtc cccggcagcg gtcgccaaag gggagaatgg attcctgacg ctagatacgg
     3841 tgccttggtc tcgagttagc ctcgccggcc gagacctggg gcaaacgcct ttgctctccg
     3901 tgagcctacc cgcgggcacc cacgtgttgc gcctccggaa tccggaactc ggcatcgaga
     3961 cgagctacga agttcagatt cagaatggga aaactaccgc tcgccggctc gggattggca
     4021 ctcacgctgg tgcctccaaa tagtcaatct gcatgatctc gaatcacgct ctggtcttca
     4081 aacggcactc atgcaaacgg cattggctgc tcttcgcctg tggcgtggtg ggtggcctgt
     4141 tcaccgcgcc aggcgccgct cacgcggagg atacggcacc ggccgcggcc gcgctgtccg
     4201 acgcgacgaa agcctacgag gaggtggatt acgcgcgagc ttacgagctg ggtcgggtcg
     4261 cgctgaatcg aggtcaagct tcagttggag atagtgtccg gctctatacg ctgctgggta
     4321 tatcggctgc ggcgttgggc aagacggacg aagcgcgtcg ctacttcatc accgcgctcg
     4381 cgatcgaccc tgcgcgcgag ctcgagcaat ccctctcccc gcgtgtccgc gcgccctatt
     4441 tggaagccaa aggcaccctg tcactttaca aggagccaat tcggctgctc gctcggatgc
     4501 agcgttctgg gacgtacctg gtggtgcgct tggaggatcc tttggctttg ataaagtcgt
     4561 tagcggttca gacgcgcaat gcctccacga cagccgcgcc cgcggacgag ccagaggtcg
     4621 agttgccggc tcagccggag aaccgagtct tgattgcctc gacgctcgcg ggggttg
//
LOCUS       DASTKU010000920        17829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_625335, whole genome shotgun sequence.
ACCESSION   DASTKU010000920 DASTKU010000000
VERSION     DASTKU010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 17829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17829
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625335"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VFQ61_32365"
     CDS             <1..404
                     /locus_tag="VFQ61_32365"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET9959244.1"
                     /translation="LADTALERKPGLPENVLQGLRAKASRSPTLIVLIASPKAGKIEA
                     WEQVATAACAGYAIVLAAHALGVGAAWKSVPFTRGKPLELLLGLGPTEEMIGWVHLGT
                     PMQASSPVRAPLDVSALTTVIDGAAPRPFRP"
     gene            754..1452
                     /locus_tag="VFQ61_32370"
     CDS             754..1452
                     /locus_tag="VFQ61_32370"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET9959245.1"
                     /translation="MKSNHSMGKFVLVNVVGLAVCLGAVACTGSSQEPPATQDQALPS
                     DAPAEAPAAPAAATPTTPSTPTPATTDAPIDPPPVDKVALTEPQIALLADLANTSEIE
                     QGQLAQKKAKAPGVQSFAGMMVRHHTQARQEQSALFKKLSITPAESSAAAQLKADAAK
                     TLDSLKQADAASFDAAYTASQVDAHQKVLDLIDKEMLPAATQPALADGLRKMKETVQS
                     HLTEAKSLQAKLNK"
     gene            complement(1615..2091)
                     /locus_tag="VFQ61_32375"
     CDS             complement(1615..2091)
                     /locus_tag="VFQ61_32375"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9959246.1"
                     /translation="MQPKNTICLWFDKDAEEAARFYAATFPDSKVTAVHKAPGDFPGG
                     SQGDVLTVEFTVLGIPCLGLNGGPIFKHSEAFSFQVATDNQEETDRYWKAIVENGGSE
                     SDCGWCKDRWGLSWQITPRVLTDALAAGGAEAKRAFEAMMTMRKIDVAKIEAARRG"
     gene            complement(2294..3589)
                     /locus_tag="VFQ61_32380"
     CDS             complement(2294..3589)
                     /locus_tag="VFQ61_32380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9959247.1"
                     /translation="MMNSAAPSLARQPNFLLYLAARACAATAVQMQGVAVGWQVYELT
                     RNALDLGWIGLAQFAPFLVLILPAGEVADRYSRRLVISSCLGLQLLCSLFLLAFSRGG
                     LTSVWPVFSALVLLGTARAFSMPAQQAILMNLVPEQSFRQAMALSSSSFHAAVIVGPT
                     LGGLLYIAGPSVVYTTVAGLLALSLALMLCTRTSQQVSTRRPPVTFASLVEGLRFVWN
                     RKPILGAISLDLFAVLLGGAVALLPAYARDILHVQASGLGLLRTAPGVGAAATAALLA
                     IFPIHRHVGRWMFGGVAVFGLATVVLGITSSFGVALAALAMTGAGDMVSVYVRHVLVQ
                     LETPDAIRGRVSAVNAVFIGASNELGEFESGIVAAALGLLPAIVVGGAATLLVTGLWM
                     RWFPMLSRMDRFPHAARDTPLSAPTIPAGAGGSVENTQS"
     gene            complement(4216..6144)
                     /locus_tag="VFQ61_32385"
     CDS             complement(4216..6144)
                     /locus_tag="VFQ61_32385"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET9959248.1"
                     /translation="MSFSRISILGSGALPLLLQLVACSARTDSEPSATRDASARAEHA
                     DPEGGESPAQANQKSTSEPLDPLSIPKWRTVLEKPPVYAPKLVTDNRGAIIRQDYRVS
                     VREDTAQMLPDGYPATRVLAYGGLVSRAGSAQPVEYRGSPGATFEMTRGIPARVEWVN
                     EITTPHFLPVDPTLHWANPNELPSPTSPFLEFPEGYPQAQSNVPYVTHLHGLEVASSS
                     DGNPEAWYTASGTQGPKYDRNVYDYPNSQPATALWYHDHTLGMTRLNVYAGLAGMYII
                     RDPRDPVANPTTGVTALPDAAHEMPLVIQDKSFNSDGTLAYPNAGFSPEHPYWTSGFD
                     GNTNVVNGKVWPEMKVERTRYRFRVLNGANTRYYNLSLSNGQPLIVIGSDGGYLEKPV
                     EAPTLLLAPGERADVLVDFSNLEPGQELILKNDSPDQLGEVVRFTVPADAPKLAAPPP
                     LPESLNEIPELTTNVPKRTVTLHSAPDGSYLLDGQMFDSAVSETPKIGATEDWDIVNL
                     TYLDHPIHLHLVQFRLVQRQGLDDAKYTEAWTALNDSALPLHQPTVKLDVDPYLVGTP
                     TPPTATEAGWKDTVVAPGMSVTRIRVRWAPQEAPMTTAPGTNAFAFDPTEGPGYVWHC
                     HMLEHEDNEMMRPLRVVK"
     gene            complement(6605..6958)
                     /locus_tag="VFQ61_32390"
     CDS             complement(6605..6958)
                     /locus_tag="VFQ61_32390"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9959249.1"
                     /translation="MENIVERYLQIWNTTDSSARRAAIEALFTRDCRYTDPIASVSGP
                     AELDTFIAGLQKQFSGVTFKLASRVDAHHDLARFSWHATAAHSAEPLAIGFDVVSLNQ
                     GRIERVLGFLDKAPG"
     gene            7061..7903
                     /locus_tag="VFQ61_32395"
     CDS             7061..7903
                     /locus_tag="VFQ61_32395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9959250.1"
                     /translation="MKPFGELLREWRERRGVSQLALACEAEVSSRHVSFLETGRAAPS
                     REMVLRLSRVLSVPLRERNVWLHAAGFAHAFAERSLDDRALSRARAAVELVLRGHEPF
                     PALAVDRHWTLVASNDAVTRLLAGVAPKLLEPPVNVLRLSLHPEGLGPRIQNFEQWRT
                     HLVERLHRQIETLADPVLVELAKELQRYPTPTSQRDRAGQTPSQPEYGGVAIPLELAT
                     DAGVLRLISTTTVFGTAVDVTLSELVLESFFPADARTAELLAPPSSTRRGRVGRAVPR
                     GSAE"
     gene            complement(7952..8863)
                     /locus_tag="VFQ61_32400"
     CDS             complement(7952..8863)
                     /locus_tag="VFQ61_32400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9959251.1"
                     /translation="MSVNDTERLRRELLLRAMFPTMTRDALVRFAEQAQPLSVATGET
                     VFERGSPPDTIYFVREGRVTMEAEGETAWTFGPRSVVGVIDAMIGRPRARSCVATQDS
                     QLLSLAVSEWLDLLEYDSNTARGSIRNFALQVHRRWPAWASATPALEEPHPRASTPSP
                     LSTYDKILALRRAAFLRPAGMQAIASLAMIAEEESLEAGQVLFPIGSEMSTLRMIVSG
                     NVLLTSENGVSVVQTAGALLGGPAALSGAPTVYSARAVTPARLLRVHEQDYYDQIEEH
                     VRLARSTLSYLVAEFERVLVRAPPTAP"
     gene            complement(8860..11517)
                     /locus_tag="VFQ61_32405"
     CDS             complement(8860..11517)
                     /locus_tag="VFQ61_32405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9959252.1"
                     /translation="MRLTLRNVVDLRPGESGVFVRAFGVLLLTVAGHTLLETARDALF
                     LARLPPRSLTYVYVMVAVGAVVIAPLSSRLARFTGARSALVISLVLAAFGAAWFRVRE
                     PSPSVVFGLYVFGTLSATLLIGQFWALAGSMFSASQGRRLFGALASGGVLGAIVAAAI
                     AASLLGSFDVRVLLAGSATAYFGAAWLATTIETEPVASTPAGGGMQKDLGRVRREPLV
                     LKLAAITGISMLAAVVIDYLFKARVAQALRPSDLAPFFAKYQLFLNIASLTLQVTVAG
                     PLVERLGVVGLAVISPTLLALGGVSTLISGAALPLVVMLKATDSTLRNSLGRVSMELF
                     WAPVEQQARLRGAVDIVISRGAQAVAGAVLFAASASGRISNFVLASAAAGLAVIWLVL
                     SWGTRKPYIELFRSALGQGNIGREWALAELDLTAVETVVEALARPDAGEVIAAMNLLA
                     ERGRVRLVPALILYHDSPDVLKRALELFGAGPRKDWYPLGRRLLDHADPSVQNAAVRA
                     FALSGADAELERAAQHEHPSVRGFAALYLAQRQGRTMQGAPLSWPLFAADSATHPLKR
                     AFIEALAANPTPDAPKLLLAFAAEPELASAVTAAMEHAADPECIPFLIERLGVADDRM
                     AAGRALVRLREPAFAALIDALRTRQLPRRVRIHIPRTISAFENDAAATELLQILRSDH
                     EGLIRYKALRGLEQLARSTTVRIASEPILQEIERNALEHLRLFALVHVIGSAGEDAER
                     LELVLVRELLEDKLHQSLDRLARLVQLAHRSDDIPTLFAALNDADRRRRAQAVEFLDA
                     LIRSLSRASVETAGLLRLVVDDLSPQERVRRSSERIGVIPHLHDALTRLSEDPDQILR
                     ELARHASTVWRSPQSRHPAAGPQALGGTQ"
     gene            11863..12195
                     /locus_tag="VFQ61_32410"
     CDS             11863..12195
                     /locus_tag="VFQ61_32410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959253.1"
                     /translation="MRARGRNLRAGVLFAGLALGTILLGGERHLAGPLRWLIAIPLGL
                     ASYALLVGAFGVCASSGLRGMRFEDYGREAILDRASRRRLRLRALIVLSLSAFIAGLF
                     AFAFANRS"
     gene            complement(12215..13252)
                     /locus_tag="VFQ61_32415"
     CDS             complement(12215..13252)
                     /locus_tag="VFQ61_32415"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HET9959254.1"
                     /translation="MHRIELRTVDRQSLNFECAPEENLVAAAARAHITLPGVCREGHC
                     GACRGTCDAGEYELLACDAGALSERDRGRGGILLCRTRPRTALSVSVPLESTQLTAGP
                     PPEFECEVERVEDLGAGVRRLELRSLPDEAGNLNCMFEPGQYLELEVPGTDRRRAYSI
                     ANAPNWEGRFEFMVRLQPNGCFSEWLVQRARTGDRLRARGPQGQFALREAGLAVRHFV
                     AGGTGVAPFFSMLRQMAEFGQTFPVRLYLGVTRERDLFGQSLLLELQGLLPNLSVEVC
                     VWQPEATWKGHRGSAAQALSRELARDGAARDSSEIYVCGPPGLVEAVRSVCDELKVGI
                     DRVHCERFLKS"
     gene            complement(13316..14971)
                     /gene="hcp"
                     /locus_tag="VFQ61_32420"
                     /gene_synonym="priS"
     CDS             complement(13316..14971)
                     /gene="hcp"
                     /locus_tag="VFQ61_32420"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF003658.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HET9959255.1"
                     /translation="MFCYQCEQTARGESQGCSSRVGNCGKTSETSDLQDLLIYAVKGI
                     AQYDLRARQLGAGDNRAGAFILYAMFTTLTNVNFDSARFVTLLREAQATLDRVRTRYH
                     QACLQQGVQAEVLTGPAALQLPEQASELLQVACLGAVQVGLHKVGEDVVGLRALVLYG
                     CKGVCAYAHHALMLGQQSEGVYSGIEQALDFLASDPENVEALLKQALDLGSLNLKVMA
                     LLDAGNTGRFGTPTPTQVRVTPIPGKAILVSGHDLADLEALLKQTAGTGIHVYTHGEM
                     LPAHSYPELKKYPHLVGNYGGAWQDQQREFASFPGPILMTSNCIIEPAPTYKRRIFTT
                     GPVGYPGVKHLAEHDFGVLIQAAKAMPGFMVDGPEQHITIGFGRDAVMGVAGDVIAAV
                     KSGAIKHFFLVGGCDGAAPGRNYYSEFVASTPKDSVVLTLGCAKYRFNRVDWGSIGGI
                     PRLLDVGQCNDSYSAIQIASALAGAFGCSVNDLPLSLVVSWFEQKAAAVLLSLLALGI
                     RKIRLGPTLPAFLTPAVLDRLVSDFQIQPVTSPEADLARSLAN"
     gene            complement(15405..16100)
                     /locus_tag="VFQ61_32425"
     CDS             complement(15405..16100)
                     /locus_tag="VFQ61_32425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET9959256.1"
                     /translation="MTRPPHHEHHRGNLSWLRAAVLGANDGIVSTSALVVGVIASGLG
                     PSMVLSSGIAGAVAGAMSMAAGEYVSVKSQADAELADLAIEKRELAEHPQLELQELAG
                     IYMQRGLSPELAQRVAEELTAKDALDAHARDELGTTDAQRARPIQAAVASGTAFSVGS
                     LLPLTGVLLAPPPLVERTTIGLTLVSLMLTGGLAAYTGRASVLRGAVRLLFWGSLAML
                     ATTLVGKLFNIAP"
     gene            16960..>17829
                     /locus_tag="VFQ61_32430"
     CDS             16960..>17829
                     /locus_tag="VFQ61_32430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9959257.1"
                     /translation="MSDSAESWELTQGALINGKYRIERVIGRGAMGLVLAATDLRLGR
                     RVALKFLLPAVASNPELRARFTREAQSAARLRNQHVVSIFGVEESGEGQPFIVMEYLD
                     GEDLATLLARRGRLPVDAAVEYVLQTCEALSEAHALGIVHRDLKPANLFLTRHPDGSP
                     WIKVCDFGIAKSAFGDTPHLTKTGEFIGTPQYSAPEQLRSSGAVDQRADIWSLGAILF
                     EMLAGRPPFQGHSLQHLAAQVCHTDPPELGGVRPDVPVGLRQVVSSCLRRDLDSRVLD
                     VAEVAERLRPYAPP"
BASE COUNT         3399 a         5483 c         5681 g         3266 t
ORIGIN      
        1 ctctcgccga cacggcgctc gaacgcaagc cggggctgcc cgagaacgtg ctacaggggc
       61 tccgggccaa ggcttcacgt tctcccacgc tcattgtgct gattgcttcg cccaaggcgg
      121 gtaagatcga ggcctgggag caggtggcca ccgcggcttg tgcgggctat gcaatcgtac
      181 tcgcggctca tgccttgggc gtcggcgcgg cctggaagag tgtgccgttt acccgtggca
      241 agcccctcga gttactcctg ggcttggggc caaccgagga gatgattggc tgggttcatc
      301 ttggaacccc gatgcaagcc tcgtcaccag tacgcgcgcc gctcgacgtg tctgcgctca
      361 ctacggtgat cgatggcgct gcgccgcgcc cctttcgacc ctgaatcggc gattcaggaa
      421 gagagcgcga atgtggatag ctgctcgcgc agcgatctgc tctccggcga atcaagcatt
      481 gagagtgttc ccttgaactc tttatgtcag ctgatgcgaa caacctagtt cgaacgtcag
      541 atcgggattg cgtgaaatcg ctcgtgcgtc tgccgctgga atccttgccg agtcattgct
      601 gcatagtgtg gcggttcgag ctcactctcg tgttgaccgc attgccagaa ccgctttcct
      661 gcagaaggcc aagtgcggca ttactgtctc actcgaccgc gataagcacc atcgtaagaa
      721 ggcggcacag cttctgcact cgcggcgcga caaatgaaat ccaatcattc gatgggtaag
      781 ttcgtattgg tcaatgtggt cggcctcgcg gtttgcctgg gcgcagttgc atgcacaggc
      841 agctcgcaag agccgcccgc gacgcaggat caggctctgc cctcggatgc gccggccgaa
      901 gcgcctgctg caccggctgc agcaaccccg acgacgccat ccactccgac accggcgacc
      961 accgacgctc ccatcgatcc gcctccggtc gataaggttg cgttgaccga gccacagatt
     1021 gcgttactgg ccgacctagc caacacctcg gaaatcgagc agggccagct cgctcagaag
     1081 aaggcgaaag ctccgggcgt acagagcttt gcgggcatga tggttcgtca ccacacacag
     1141 gcgcggcagg aacaaagcgc gctattcaag aagctcagca tcacgccagc cgagagcagt
     1201 gcagccgccc agctgaaagc cgacgctgcg aagactctgg acagtctcaa gcaagcggat
     1261 gcagcgtctt ttgacgcagc ctatacagcg agccaggttg atgctcacca gaaggtgctc
     1321 gacctgatcg acaaagagat gttacccgcc gcaacccagc cggcgctcgc ggatggtctg
     1381 cgcaagatga aagaaacggt ccagagtcac ctaaccgaag ccaaatcctt gcaagccaaa
     1441 ctcaacaagt gacactcccc tggttcggcc ccagttcggg gctctagaat cgtgcaggac
     1501 ctcgggttct gcacgattct agaactcacg ctcgccacgc gcggctacgc cattcgatcg
     1561 cgagtaccgg cgagccgatt cgagcgctct atccgcgcgg ctgcgctccg cgcgtcaacc
     1621 gcgtcgggcg gcctcgattt tggcgacgtc gatcttgcgc atggtcatca tagcttcgaa
     1681 agcgcgtttg gcctcggcac caccggccgc gagcgcgtcc gtaagtacgc gcggagtgat
     1741 ttgccaagag aggccccagc gatctttgca ccagccacag tcgctctccg agcccccatt
     1801 ttcgacgatc gctttccagt aacggtctgt ctcttcctga ttgtcggtcg cgacctggaa
     1861 agagaaggcc tcgctatgtt tgaatattgg accgccgttg agaccgagac agggaatgcc
     1921 caaaaccgtg aactcgaccg tgaggacatc gccttggctt cccccgggaa aatcgccggg
     1981 agccttgtgc acagcggtta ctttgctgtc cgggaaagtg gcggcgtaga aacgggccgc
     2041 ttcttctgca tccttgtcga accagagaca aatcgtgttc ttcggctgca tttcgtgtgc
     2101 tcctcgttga tgtggacgtc gtggggctgc cagggttgag cagctgccag gaccttactg
     2161 tcataccgga cctgcagcgc aagccttgtg agcggttgtc gggctcgaag tcgttcggcg
     2221 ggtcgggttg ttcgactctt ttgaagttgg cttttcgccc ggaccctccg aagcacgcga
     2281 gcatcgtccg atctcaggac tgagtgtttt ccactgagcc accagcgcct gccggaatgg
     2341 ttggagccga tagcggtgtg tcccgagccg cgtgcggaaa gcgatccatg cggctcaaca
     2401 tcggaaacca acgcatccag agtcccgtga cgagcagggt cgccgcgccg ccgactacga
     2461 ttgcgggcaa caagccaagc gcagcagcga cgatgcctga ctcgaattct ccgagttcgt
     2521 tcgacgcgcc gatgaatacg gcgttcaccg cgctcacgcg accacgaatc gcatcgggag
     2581 tctcgagttg cacgagtacg tggcgtacgt agacgcttac catgtcacca gcgcccgtca
     2641 ttgcgagcgc cgccagggcc accccgaaac tcgaggtgat gccgaggacc acggtggcga
     2701 gaccaaacac ggccactcca ccaaacatcc aacgacccac gtgtcgatgg attgggaata
     2761 tggccaagag tgcggcggtc gcggccgcac ccacgcccgg agcggtacgg agcaaaccca
     2821 agccgctggc ttgcacatgg agaatgtcgc gcgcataggc gggtaagagc gctacggcgc
     2881 cccccagcag caccgcgaat agatcgagcg agatagcgcc aagaatcggc ttgcgattcc
     2941 agacgaagcg gagcccctcg accaggctcg caaacgtgac ggggggtcga cgcgtggaga
     3001 cctgctgcga ggtgcgcgtg cacagcatca gcgcgagcga gagcgcgagc aggccggcaa
     3061 cagtcgtata aaccacgctc ggtcccgcga tgtacagcag cccacccaat gttggtccga
     3121 cgatcaccgc cgcgtgaaag ctggacgagc tgagcgccat cgcctgacga aaactctgct
     3181 caggcaccag attcatcagg atcgcctgct gagccggcat gctgaaggct cgagccgtac
     3241 cgagcaaaac cagcgcactg aacacgggcc atacactcgt gagcccgccg cgggagaagg
     3301 ccaacaggaa caaggaacac aggagttgaa ggcccagaca ggagctaatc accaggcggc
     3361 ggctatagcg atcggccacc tctcccgccg gtaggatcag caccaagaac ggcgcgaact
     3421 gagcgaggcc gatccagcca agatccagtg cattgcgagt caactcgtag acctgccagc
     3481 ccaccgcaac tccttgcatt tgcaccgcgg tcgccgcgca ggcgcgagcg gcaaggtaga
     3541 gcaggaagtt gggttgacgt gcgagcgacg gagccgcgga attcatcatc agtgctgccg
     3601 atccgttgcg ctaggcattg cacgaaacct cgtcggcggc cgcaagcaga ctgcgctaag
     3661 caaggctcat tggtagcggc cgcagggacc tgtcgcggcg caccggcgct cagcgcgtca
     3721 ttgacacaca gcctggccgc cataaagtgc cgcctttgcg agcaatgacg cgaaagaaca
     3781 ggggaccgaa ccttcctaaa gaagaggaag aggccgaaga ggaagagggc agcttcttac
     3841 ttgggtttgt ttctggggca gtgggaggag tcgttgctat caccttggtg attcggcgtg
     3901 ccaagcgact cacccgttta ggcgtttatg tcggggcggc cgttcagttg ctctacctgg
     3961 ttgttcgaca atgagccttt tcgcgggcca gcagcggggc tggcgcgttg gagcgcgatg
     4021 aatcggggtt agcgactgcg ctccggtgcg gacgggacgc ctgtcgcgct cgttgttctg
     4081 tggcccaagc aaatctcgtc tgtcggtcga cagcaccccg cgggcggcgc gttgccgccg
     4141 tctgcgcgct cccgttgtcc acggtccgcg atctcgaacg accgcgaacc gtgagtacgg
     4201 aggcgttgcc aaaacctact tcacgactcg aagcggccgc atcatctcgt tgtcttcgtg
     4261 ctcgagcatg tggcaatgcc acacgtaacc tgggccctcc gtcgggtcga aagcgaaggc
     4321 gttagttccg ggcgcggtgg tcatgggcgc ctcttgcgga gcccaacgca cacggatccg
     4381 tgtaacggac attcccggtg ctaccacggt gtctttccag cccgcttcgg tggccgtggg
     4441 cggggtcggg gtgcccacta gatatggatc gacgtcgagt ttgaccgtcg gttggtgaag
     4501 gggcagcgcg ctgtcattga gcgcagtcca cgcttcagtg tacttcgcgt cgtcgagccc
     4561 ttggcgctgt acgagcctga attgaacgag gtggagatga atgggatggt ccaagtaggt
     4621 gaggttgacg atatcccaat cttcagtggc cccgatcttg ggggtttcgc tgactgcgga
     4681 gtcgaacatc tgcccgtcga ggaggtagct gccgtcaggg gccgagtgca gggttacggt
     4741 gcgtttaggc acgttggtcg tgagctcggg aatctcgttc aagctttccg ggagtggtgg
     4801 cggggcggcg agcttggggg cgtcagccgg taccgtaaaa cgcaccactt ccccgagctg
     4861 gtccggtgag tcgttcttga ggatcagctc ttggccgggt tctaggttgg aaaagtcgac
     4921 cagaacgtcg gcgcgctcgc cgggggcgag caatagcgtc ggggcttcga ccggcttttc
     4981 gaggtagccg ccgtcggagc cgatcacgat gagcggctgt ccgttggaga gtgaaagatt
     5041 gtagtagcga gtattggcgc cattcaaaac gcggaatcgg tatctcgtgc gctcgacctt
     5101 catctcgggc cagactttgc cgttcactac gttggtgttg ccgtcgaagc cgctggtcca
     5161 gtaggggtgc tcgggcgaga agccggcgtt ggggtaagcg agggtcccat cgctattgaa
     5221 ggacttgtct tgaatgacga gcggcatctc atgggcagcg tccgggagtg ccgttacacc
     5281 cgtggtcggg ttcgcaaccg ggtctcgcgg gtcacgaatg atgtacatgc cggcgagtcc
     5341 cgcgtacaca ttgagacgag tcatgcccaa cgtgtggtca tgataccaga gggccgtcgc
     5401 aggttgagag ttggggtaat cgtaaacgtt gcggtcgtac ttcgggcctt gcgtgccgga
     5461 ggcggtgtac caggcctcgg gattgccatc ggaactggag gcgacttcca ggccatgcag
     5521 gtgtgttacg tacggcacgt tggactgcgc ttgcggatag ccctcaggga actccaagaa
     5581 cgggctcgtg ggggatggaa gctcgttggg attggcccag tgcagggtcg gatccacggg
     5641 cagaaaatgg ggcgtcgtga tctcgttcac ccactccacg cgcgcgggga tgccgcgggt
     5701 catttcgaag gtcgcaccgg ggctgccgcg atactcgacg ggttgggcgg agccggcacg
     5761 acttacgagc cctccgtacg cgagcacgcg cgttgccgga taaccgtcgg gcagcatctg
     5821 cgccgtgtcc tctcgaaccg acacgcggta gtcctggcgg atgatcgcgc ctcgattgtc
     5881 ggtcacgagc ttgggcgcgt agacgggagg cttctccagc acggttcgcc acttgggaat
     5941 actaagcgga tcgagcggct cgctggtgct cttctgattg gcttgggccg gagattcgcc
     6001 gccctctgga tccgcgtgct ccgcgcgagc actcgcatcg cgggtggccg aaggctcgct
     6061 gtcggttcgc gcgctacagg cgacgagttg aagaagaagt ggcagcgcgc cagaacccaa
     6121 gatcgaaata cgactgaatg acatgatgac tcccggtggt gattggaatg gccgaaccca
     6181 acccagagcc ccggccccga atggcgagca cgaatcgaag cgacacgaat cgagcttccg
     6241 ccggaatact agtgttccga ggggtgatcg ctcttcagcg tcgttgatgg gactctggaa
     6301 ggccgcagag cggccgactc gtgcatcgtg ttaggctcgc caaaaacggg tgatagccgt
     6361 catcgattca cgccccccgc ggcactcgta gtcattcgat gcgatcgttc ggagtgcaac
     6421 cactcggcgg tcaggggggg ccccttcaca ttccagaact tccgaatgca aagacgcccc
     6481 gaatagtatc ctccagttca cgcggcccgt tctcatcaga gcttgcgggc aagctggctt
     6541 gaacttgcag ttcgcgcccc tcagtccgaa gtccgcgcga gatcagttca ggccgactcg
     6601 ggcatcagcc tggggccttg tctagaaatc cgagcacccg ctcgatgcgg ccttggttta
     6661 ggctcaccac atcgaaccca atcgcgagcg gctccgcgga gtgggcagcg gtggcgtgcc
     6721 agctgaagcg agccaggtcg tgatgggcat cgacgcggct cgcgagtttg aacgtgactc
     6781 cgctaaattg cttttgcagc ccggcaatga acgtatcgag ctctgccggt ccgctgacgg
     6841 aagcgatggg atcggtgtag cggcagtccc gagtgaagag cgcctcgatc gccgcgcgtc
     6901 gcgcggacga gtcggtcgtg ttccaaatct gcagataacg ttcgacgatg ttttccatgt
     6961 tcgtctcctt cgccgcagaa catgcgccag ccgcgcgtgc gcgccgatta ccttcgaggt
     7021 aatagtgcgc aacacgttgg cgagtacgat gcggaaacgg atgaagcctt ttggtgagct
     7081 cctgcgagag tggagagagc ggcgtggggt gagccagctg gcgctggctt gcgaggcaga
     7141 ggtgtcgtct cgccacgtga gttttctaga gacggggcgc gcggcgccca gtcgggagat
     7201 ggtgcttcgg ctgtcacgcg tgttgtcggt ccccttgcgc gagcgcaatg tctggttgca
     7261 tgcggcgggc ttcgcgcatg ccttcgcgga gcgctcactc gacgatcggg ctctttcgcg
     7321 ggctcgcgcg gcggtcgagc tggttttgag aggacatgag ccgtttccgg cgctggccgt
     7381 ggatcgacac tggacgctgg tggcgtcgaa tgatgcggtt acacggctgc tcgccggggt
     7441 cgctcccaaa ctgctcgagc cgccggtcaa cgtgttgcga ctcagcctcc atcccgaagg
     7501 gctcgggcca cgtattcaga acttcgaaca gtggcggacg cacctggtgg agcgactgca
     7561 ccgtcagatc gagacgctgg cggatccggt actcgttgag cttgcaaagg agctacaacg
     7621 ctatcccacc cccacatcgc aacgcgaccg ggctggccag acgcctagtc agcccgagta
     7681 cgggggcgtt gcgattccgc tcgagcttgc gactgatgcc ggcgttctac gtctaatcag
     7741 cactacgacc gtgttcggga cagccgtcga cgtgacgttg tccgagctcg tgctcgagtc
     7801 tttttttccg gcggatgcga ggacggcgga gctcttggcg ccgccgagca gcacgcgccg
     7861 gggtcgagtg gggcgagcgg tgccgcgcgg gagcgctgag tagacgtcag aaggcgtaat
     7921 ccggcagctc gaattcactc gccgactgtt cctatggtgc cgtggggggc gcgcgcacta
     7981 gaacccgttc gaactccgcc accagatagg agagggtact ccgtgcgagt cgcacgtgct
     8041 cttcgatttg atcgtagtag tcctgctcgt gcacgcgcag taaccgggcc ggagtcacgg
     8101 ctcgtgcgga atagacggtc ggtgccccgg agagcgccgc gggcccgccc agtagcgcgc
     8161 ccgcggtctg caccacgctc actccatttt cggatgtcag caacacattg ccactaacga
     8221 tcatgcgcag ggtgctcatt tcgctgccga tcgggaatag aacttggccc gcttcgagcg
     8281 actcttcttc ggcaatcatg gccaagctcg caatggcctg catcccggcg gggcgaagga
     8341 acgcggcccg ccgcaaggcc aggatcttat cgtaagtgga gagcggactc ggcgtcgatg
     8401 ctcttggatg tggctcttct agcgcagggg tggcgctcgc ccaggctggc caacgtcggt
     8461 gaacttgcag cgcgaagttc cggatcgagc cgcgcgcggt gtttgagtca tactccaata
     8521 gatcgagcca ctcgctgacg gccaagctga gtaattgact atcttgagtg gccacgcagg
     8581 aacgggcacg cgggcgaccg atcatcgcgt cgatgacacc cacgacggag cgcggtccga
     8641 acgtccacgc ggtctcgcct tcggcctcca tggtgacccg cccctcgcga acgaagtaga
     8701 tggtatcagg cgggctgccc cgctcgaaca cggtctcgcc ggtggctacg ctcaagggct
     8761 gagcttgttc cgcgaagcgt acgagtgcat cccgggtcat ggtcgggaac atggcccgaa
     8821 gcagcagctc acgacgcaag cgttcggtat cgttcacgct cattgcgtgc ctccgagcgc
     8881 ctgcgggccg gcagcgggat gccgcgattg cgggcttcgc cagaccgtgc ttgcgtggcg
     8941 cgctagctcg cgcaggatct ggtcgggatc ttcgctgagt cgagtgagcg cgtcgtgaag
     9001 gtggggaatg actccaatcc gctcggagga gcgccggact cgctcttgtg gcgacaggtc
     9061 atcgaccacg agccgcagca gccccgccgt ttcgaccgag gcgcggctca aactacgaat
     9121 caaggcgtcg aggaactcca cggcttgagc gcgtcgcctg cggtccgcgt cgttcagagc
     9181 cgcgaacaag gttgggatat catcactgcg gtgcgcgagc tgcacgagcc gtgcgagtcg
     9241 atccaaggac tgatgtaact tgtcctcgag cagttcgcgc accagcacga gctcgagccg
     9301 ctcggcgtcc tccccggcgc tcccgatcac gtgcaccaga gcgaacagac gtagatgctc
     9361 gagcgcgttc cgctcgattt cctgcaggat aggctcgctc gcaatgcgca cggtggtgct
     9421 tcgagcgagc tgctccaggc cccgcagagc cttgtagcga atcaagcctt cgtgatcgct
     9481 gcgtaagatc tggagcaact cggtcgcggc ggcgtcgttc tcgaaggccg atatcgtcct
     9541 cgggatgtgg atgcgaacgc gccgcgggag ctgccgggtg cggagcgcgt cgatcaacgc
     9601 cgcgaaggcg ggttcgcgaa gacgcaccaa agctcgcccc gcagccatgc gatcgtcggc
     9661 tacgccgagc cgctcaatca agaacggaat acactctgga tcggccgcgt gctccatcgc
     9721 tgccgtgacg gcgctggcga gctcaggctc ggccgcgaac gccaacagta acttcggtgc
     9781 atcgggcgtc gggttggccg ccaacgcttc gatgaaggcg cgtttgagcg ggtgggttgc
     9841 cgaatcggcc gcgaacagcg gccagctcaa gggggctcct tgcatggtcc ggccttgacg
     9901 ttgcgcgaga tatagggccg caaagccacg cacgctcggg tgctcgtgtt gggctgctcg
     9961 ctccaattct gcgtccgccc cggagagcgc aaaggcgcgc acggcggcgt tctgtacact
    10021 cgggtcggcg tggtccagca ggcgtcgccc cagcggatac caatccttgc gcgggccggc
    10081 accgaacaac tccagagcac gtttcagcac gtcgggtgaa tcgtgataaa gaatcaaggc
    10141 cgggacgagg cgcacgcggc cgcgctcggc gagcagattc atcgccgcga tcacctcacc
    10201 cgcatcgggt cgtgccaggg cttcgaccac ggtctctacc gccgtcagat cgagctcggc
    10261 cagcgcccac tcgcgaccga tgttgccctg gcccagcgcg ctacgaaaca actcgatata
    10321 aggctttcga gtaccccagc tcagcacgag ccaaatcaca gccaagcccg cggcggcgct
    10381 cgccaatacg aagttggaaa ttcggccact ggccgaggcc gcaaacaaga ccgcgcccgc
    10441 caccgcttgg gccccgcgtg aaatcacgat atcgaccgcg ccgcgcagac gagcttgttg
    10501 ctcgacgggc gcccagaaca actccatact cactctaccg agcgagttcc gcaacgtgga
    10561 gtcggtggct ttgagcatta ccaccaaagg cagagccgct ccggatatca acgtcgagac
    10621 tccgcccaaa gcgagcagtg tgggcgagat tacggccagt cccaccaccc ccaagcgctc
    10681 tacgaggggc cccgccaccg tcacctgcag cgttagagac gcgatattca ggaacaactg
    10741 gtatttggca aagaacggcg ccaggtcgct ggggcggagg gcttgagcca cccgcgcctt
    10801 gaacaagtaa tcgatgacga cggcggccag catcgaaatc ccagtgatgg ccgcgagctt
    10861 caagaccaac ggctcgcgtc gcacgcgccc gagatctttc tgcatccccc cgccggcggg
    10921 ggtgctcgcg actggttctg tttcgatcgt ggttgcgagc caggccgcgc cgaaataggc
    10981 agtcgccgag cccgcgagca gcacgcgcac atcgaagctc cccagcagcg aggccgcgat
    11041 cgcggcggcc acgatggcgc ccaacactcc tcccgaagca agcgctccga agagccggcg
    11101 gccctgggaa gccgaaaaca tgctgcccgc gagcgcccaa aactggccga ttaacagcgt
    11161 cgccgagagc gtgccgaaca cgtaaagacc gaacaccacc gaggggctcg gttcacgcac
    11221 ccgaaaccag gccgcaccga acgcggcgag cacgagtgat atgaccaagg cactgcgggc
    11281 ccccgtgaaa cgcgcgaggc ggctgctcaa aggcgcaatc accaccgcac ccaccgcgac
    11341 catcacgtag acgtaggtca aagagcgggg aggcaggcgc gccagaaaca aagcgtctcg
    11401 ggcggtctcg agcagcgtgt gcccggcaac cgtcaggagt aatacgccga acgcacgcac
    11461 gaacacgccg ctctcgccgg ggcgtaggtc gacgacattc cgcagggtta gacgcatgcg
    11521 gcgctgcttc tcatccggcc ttgctcctca tccagtccta tttctcatcc agctctcaac
    11581 ctcacttggc ccctactctc acttgggaag cccttgcggt tccagaactg taagaaagtt
    11641 cgttccggat tgggggggga cggcattctc tccaacgcgc gagcggacgt gtgcctgctc
    11701 gcgcctacat tgtgccccgg ctcgtcgagg gagccggcga tgcccacccg acgggaccgc
    11761 ggcgagcttg ttggctcggg gacagggaaa cgcttgccgc tcatgggccg ggaagttagc
    11821 tttaaccgca tgctgccgca ctaccccagg catacgctcg gcatgcgggc ccgtggtaga
    11881 aacttgcgtg caggggtgct gttcgccggc ctggccctcg gcaccatctt gctcggcgga
    11941 gagcgccacc tggcgggacc attgcgctgg ttgatcgcga tcccactagg cctcgcgagt
    12001 tatgcgttac tggtcggagc gttcggagtc tgcgcgtcga gtgggctgcg cggtatgcgc
    12061 ttcgaagact acgggcgcga agccattttg gatcgggcga gccgccgaag gctgcggctc
    12121 cgggcgctga tcgtgctttc gctcagtgct ttcatcgctg gtctgtttgc gttcgcgttc
    12181 gccaatcgca gctgacccca tcggaatcga cggctcagct cttcaggaag cgttcgcagt
    12241 gaacgcgatc gataccgacc ttcagctcgt cgcagacgga gcgcaccgcc tcgaccagcc
    12301 ccggcggccc gcaaacgtag atttcggagc tatctcgcgc ggcgccgtct cgggcgagtt
    12361 cacgggatag cgcttgcgcg gccgagccgc gatgaccctt ccaagttgcc tcgggctgcc
    12421 acacgcacac ttcgacactg aggttgggga gcaagccctg gagctcgagc agcagggact
    12481 gtccgaacag gtcgcgttcg cgcgtgacac caagatagag cctgactggg aacgtctgtc
    12541 caaattccgc catctggcgg agcatcgaga agaagggcgc gactccggtg ccgcccgcca
    12601 cgaagtgtcg caccgccagg cctgcttcgc gcagagcgaa ttggccttgg ggcccacgcg
    12661 cacgcaagcg atccccggtt cgcgcccgtt gcacgagcca ctccgaaaaa catccgttag
    12721 gttggaggcg aaccatgaat tcgaatcgcc cctcccaatt cggtgcattc gcgatcgaat
    12781 aagctcgacg tcgatcggta cccggtacct ccaactcgag atactggcca ggttcgaaca
    12841 tacaattcag attgccggct tcgtccggca gcgagcgcaa ctccagacgt cgcacgcctg
    12901 cgccgagatc ttcgactcgt tcgacctcac actcgaactc gggtgggggg cctgcggtca
    12961 actgggtgga ctcgagcggc acgctcaccg agagcgctgt gcgcggtcgc gtgcggcaca
    13021 agagtatccc gccgcggccc cggtcgcgct cggacagggc gccagcgtca cacgccaaga
    13081 gctcgtactc gccggcgtcg cacgtgcctc ggcacgcgcc gcagtggcct tcgcggcaca
    13141 ctcctggcaa cgtgatgtgc gcgcgagcgg ccgcggccac caaattctct tcgggggcac
    13201 attcgaagtt caacgattgt cgatcgacgg tcctcagctc gatgcgatgc atgcgtccct
    13261 gcttggaagg ccggtgtggg tgtccacacc ggcccatctt tcgtttcgtt gcggttcagt
    13321 tcgcgagcga acgggccaaa tctgcttcag gcgacgtaac gggctgaatc tgaaagtcac
    13381 tcaccagccg atccaaaacc gcaggtgtca ggaacgcagg caacgtgggg cccagccgaa
    13441 tcttgcggat cccgagcgcc agcaaggaca gcaacaccgc cgccgccttt tgttcgaacc
    13501 acgacaccac caaagacagc ggcagatcat tcacgctgca gccgaaggcg cccgctaacg
    13561 cggaggcaat ctgaatggca gaatagctat cgttgcactg gccaacatcg agcaatcgcg
    13621 gaatcccacc gatgcttccc cagtccacac gattgaagcg atacttggca catccgagcg
    13681 tcaagaccac gctgtctttg ggcgtactcg caacgaactc ggaatagtag tttcgacccg
    13741 gggccgcccc gtcgcagccg ccgaccaaga agaagtgctt gattgcgccg ctctttaccg
    13801 cggcgatcac atctccggcc acgcccatca ccgcgtctcg tccgaacccg atcgtgatgt
    13861 gctgttcggg cccatcgacc atgaacccgg gcatggcctt ggccgcctga atcaacacgc
    13921 cgaaatcgtg ctcagctagg tgctttacgc cagggtaacc cacggggccc gtcgtaaaga
    13981 tcctgcgttt gtaggtcggc gcgggctcga tgatgcagtt cgacgtcatc aggatcggcc
    14041 ctgggaagct cgcgaactca cgctgttggt cctgccaagc gccaccataa ttgccgacga
    14101 gatgaggata cttcttcagc tcgggatatg agtgcgctgg caacatctca ccatgcgtat
    14161 agacatggat gccagtacca gccgtttgct tcaatagagc ctcgagatcg gccagatcgt
    14221 ggccactgac gaggattgcc ttgccgggga taggggtgac tcggacctgc gtgggagttg
    14281 gagtgccgaa ccggcccgta ttgccggcat cgagcagcgc catcaccttc aggttcaggc
    14341 tgccgagatc gagcgcctgc ttgagcaagg cctcgacgtt ctccggatca ctggccagga
    14401 agtccaacgc ttgctcgatg ccgctgtaca cgccctcgct ctgctgaccc aacatcaaag
    14461 catgatgcgc atacgcgcat acccccttgc acccgtaaag tacgagtgca cgcagcccca
    14521 ccacgtcttc gcccacctta tggagcccca cctgtacggc gcccaggcag gcgacctgga
    14581 gtagctccga agcctgttcg gggagctgca acgcggcggg ccccgtcaac acctcggctt
    14641 gcacaccctg ttggagacaa gcctgatgat accgagtacg cactcgatcg agcgtggctt
    14701 gcgcctcgcg cagcaaggta acgaagcgtg cagagtcgaa gttcacgttc gtaagcgtcg
    14761 taaacatcgc gtacaggatg aaagctcccg cgcggttgtc ccccgcaccg agttgccgag
    14821 ccctcagatc gtattgtgcg atgcctttga ctgcgtagat caggagatcc tgcaagtccg
    14881 atgtctcgga cgtttttcca caattcccca ctcgggacga acaaccctgg gactctcctc
    14941 gcgcggtctg ctcacactga taacaaaaca tgggctctcc tcctgatcaa actctgcgga
    15001 tcgatgcgcg agcacgaatc cacagctgat tgcgctagcg caaatctatg ccgagcgtct
    15061 ccaaaccggc acgcagaata ccacaatgat agccaagtcc agttcgaatc gtggcgtcgc
    15121 ggtgccggtg ccagtctgtt gacgaacctc ggaaggttcg gcggttttgc gcaacatttg
    15181 ccagcccaat gcagggaact tccgtgcgcg cgcagataac gcgcactctc cgagcgtcct
    15241 tgaagcgcag cgttgaagtt ttgcggagag gcggctcggg tagtgcgtcc ccgcccagag
    15301 cacgacatga catcaatcag tcgagcggaa tggagttggc gctcgacgaa acagcagcaa
    15361 gaggaggacg gatggcgcgc agggtaaagg agtatcgggg gcggttacgg cgcgatgttg
    15421 aataacttac cgacgagggt agttgcgagc atcgcgaggg agccccagaa caacaaccga
    15481 acggcgcccc gcagaacgga ggctcttccg gtatatgcgg cgaggccgcc cgtgagcatt
    15541 agactgacga gcgtgagtcc gattgtggtt cgttcgacga gcggaggtgg tgctagcagc
    15601 accccggtga ggggcagcag tgagccgacg gagaaggcgg tgccagatgc gaccgcagcc
    15661 tggattgggc gggctctttg ggcgtcagta gtgccgagtt catctcgcgc atgggcatcg
    15721 agcgcgtcct tggccgtgag ttcttccgcg acgcgctgag cgagttcggg cgaaagcccg
    15781 cgctgcatgt agatgcccgc gagctcctgc agctcgagct gcgggtgctc ggcgagttca
    15841 cgcttctcga tggcgagatc tgcgagttcg gcatcggctt gtgacttgac ggaaacatac
    15901 tcccccgccg ccatcgacat cgcgcccgct acggcccctg cgattccact gctcagcacc
    15961 atcgacggcc cgagcccgct cgcgataacg cccacgacga gcgcggacgt ggatacgata
    16021 ccgtcgttgg ctcccaaaac cgcggcgcgt aaccagctca aattgccgcg atggtgttca
    16081 tggtgggggg ggcgagtcat cgttcgatga cggacgttat cctgattcga cggcgaacgc
    16141 acgacatggc gaagcaacca ccctgcgccc tttcgcccgc agggcctaga cgtaaaccaa
    16201 tcgtggcgag cggcggcgga gcacgcgaac acacacgaca ttgcgggagt ggatcgggcc
    16261 gcgatgtaac gggcgcgaca cgagtcgcgc ggtctcaagc gcgcagcccc ttgcaaccca
    16321 cagcgctccc cgcaccgcgc aggccctcgc aataaccgac gtcgctcgcg aaggcgagtc
    16381 atcgttcggc ggagtggtat tcgggcactc tgcgcgccat cacgcgttac gtggatggcc
    16441 tgcgcggtat cgtgagtaga gcgagaggct cggactgcgc gattgattgc atccgacgcg
    16501 agtcgcgccc ggaaacagct tggccaacag tcatattgtc accgcaggtt ggtactaaac
    16561 aaaatatatt catgttgccg tgcgttcgag agagtgaacg agagcagtgg cgttgcagat
    16621 tccgggtgcg ctaccgcaag gcctgtagct tcgaggaggg cctgtctgca ccctgcaccc
    16681 tgcaatcgtg cagtcgtgca gtcgtgcagc gcccggttcg tctagtcaat gtttctattg
    16741 cgctcccgcg tgggtcgaca ggcggcgcgt gcgcattgat tctctcgctg gtgttagctc
    16801 tcggaggggt gtcgaaaacc gtcgaatcag gaagctcgcc gcagcactac cgagctgcgc
    16861 ttcggtgccg tattctgttt tgcacggagc agacgagcgc ggctgcggcg tggcgcgccg
    16921 gaacgagtca acgtcagggt ttcgagggag cttctggcca tgagtgacag tgcagaatcg
    16981 tgggagctca ctcaaggcgc gttgattaac ggtaagtatc ggatcgagcg agtgatcgga
    17041 cgcggcgcga tgggcttggt gttggcggcc acggatctgc gactgggacg tcgcgttgcg
    17101 ctcaagtttc ttcttccagc ggtggctagc aatcctgagt tgcgggctcg tttcacgcgc
    17161 gaggcgcagt ctgccgcgag attacggaat caacatgtcg tgagtatctt tggcgtggag
    17221 gaatcggggg aggggcaacc cttcatcgtg atggagtacc tcgatgggga ggatctcgcg
    17281 acgctgttgg ctcgtcgggg aaggctaccg gtcgatgccg cggtcgagta tgtcctgcaa
    17341 acgtgtgaag cgctttcgga ggcgcacgct ctggggatcg ttcatcggga tttgaagcca
    17401 gccaacctgt tcctgacgcg acaccctgac ggttcgcctt ggatcaaagt ctgtgacttc
    17461 ggaatcgcca agtcggcgtt tggtgatact cctcacctga ccaagactgg tgagttcatt
    17521 ggtacgccgc agtacagcgc gccggagcag ctccgctcgt cgggagccgt agaccagcgt
    17581 gccgatatct ggtctttggg cgcgattctg ttcgagatgc tcgcgggacg gccgccgttt
    17641 caagggcatt cgctgcaaca cctcgcggcg caggtctgtc acaccgaccc gcccgaactc
    17701 gggggtgttc gtccggatgt gcccgtcgga ttgcggcaag tcgtttcgag ttgtttgcga
    17761 agggacctgg attcccgtgt tctcgatgta gccgaggtag ccgaacggct tcgaccctat
    17821 gcacccccg
//
LOCUS       DASTKU010000921         5148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_625428, whole genome shotgun sequence.
ACCESSION   DASTKU010000921 DASTKU010000000
VERSION     DASTKU010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625428"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            543..1184
                     /locus_tag="VFQ61_32435"
     CDS             543..1184
                     /locus_tag="VFQ61_32435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959258.1"
                     /translation="MQGYPRLGKDAPAIHMTACQHEGTSQFPHAGPWLLAFADAWHSE
                     PSTSTSHTASALDAPSALFQLRTPGMMAALDQLGTSLLVIAGPEVWQLQRGCYQTSRP
                     APEELRAASRAYAVDCNPPTGAFFLVDGNGTLRFAHYTSLGKTPAASVLTEAVLSAWR
                     EFSGRPGTGAERNERRISCIASELMHALVAAPSPAEPRHTGTFASTSAAALSS"
     gene            complement(1298..1981)
                     /locus_tag="VFQ61_32440"
     CDS             complement(1298..1981)
                     /locus_tag="VFQ61_32440"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET9959259.1"
                     /translation="MSSSSSRLSARDLARVPVFPLPNVALFPGASLPLHVFEPRYRQM
                     TEDILAGDRLIVVARLCEGYEADYEGKPAFHPVCGIGRVVQDEARPDGRYDLVVQGLS
                     RVRVTRELPTSRAYRVVSVELLPDQTPSDAYALLSWQRELAGLWEKLRPYLPESVRGL
                     LERAYEVPSAGARIDMLAAAVVADPDERQRFLEELDPSQRSWLLVEKLRNLLSALSAP
                     DVRAQSRLN"
     gene            complement(1992..3668)
                     /locus_tag="VFQ61_32445"
     CDS             complement(1992..3668)
                     /locus_tag="VFQ61_32445"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein"
                     /protein_id="HET9959260.1"
                     /translation="MASQTADLLHPEPRLWLDEAALQQALTFAFAAGSTGEDFDRLLN
                     AAKLAPSRFRSDSFARDLFVSDVVQKCMVIRVKKRERNYHRGLLIRCLCQPPIDLDTV
                     VFRQGVLRELEQRPELRTAMERSWLQIQELISRLEAGERGKRYDVIARRVDILRDVKH
                     AIELLHDGFSHATSGLARAAQFATQVRDSKSYRDLLAVLDHESHLATVELRVRLAHDG
                     GLRGFEITSARENRENPFHVAPLQRFLTRLRFFFRGYRVREAEILGRLINQVFDGVQS
                     AVIGLFQIGLHFEFYLAALALKARAAELGLQMCLPEIVQRGHADSETRLEALFNPLLL
                     LEGKAPIPCTLEVGPSALTIITGPNSGGKTRLLEALGLSQLLGQAGMFVPAARARLAF
                     RDGLFVSLIQVASAEHQEGRLGTELRRIRHTFEELDVNSLVILDELCSGTNPSEGEGI
                     FELVVSLLCELEPQAFITTHFLEFAARLARDRPVRNLEFLQVELDARSEPTYRFVTGV
                     ARTSLAGKTAERLGVTREALTALVREKRRTLSTPPADPDQEAAAAAIPKL"
     gene            4067..5014
                     /locus_tag="VFQ61_32450"
     CDS             4067..5014
                     /locus_tag="VFQ61_32450"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET9959261.1"
                     /translation="MWHWFWQGPRARELREQRASTRVLELSRRARVALELATHAEDPR
                     EPLRAGDAKPLAAELRREAAYWALRAYAENQGAVLADNDGLNAVAKLVPQELLDSAFE
                     ADAAVQLSELELTPFQRLSDLDPVVMARFSDRAERFTRILLNVTDAPAIELERLWLRR
                     LLRVGAIGALLGLTALTAIKLRDWQEDARDLAKGRAYRVSSGALGCRSPEQECSESPF
                     FFFHTAEEDKPWVEIDLGLVRTVSGVRLVNRTDCCTERGTPLVIELSVDQQNFREVAK
                     QTNEFRSVKLEFPTSRARFVRVRALNRTILHLQRVRVLP"
BASE COUNT          997 a         1646 c         1609 g          896 t
ORIGIN      
        1 gcgcaagccg agcgccggct gctgtccgcg cgcttgaccc agtgtttcga tctcacaagc
       61 cgaatggtca gacctgtgac gatcttttcg gcgaaaagcg cgacggagcc gcacggtaac
      121 ccaccccgta cgttcgggtc cgcagatacg ccaacgtaaa ttggtatcga ttccgaaacg
      181 tcacctacgt ggcgtggggc aatgagcgcc gccgggacct atatgaggct cgcccgctct
      241 aggcgggagc gctcgcgcgc cgcgaaatac ccacgcaaac gtcgcatgat ttttcgacac
      301 ttcgagcgcg aagagcgtaa cctgcacctc catctagctt gttcttgctc gcgcatgact
      361 gcgcgcgcga acgcgagtcg aacgcaatct ttactaacag tcgggtgcgg cgcaatcgta
      421 cacgcaaacg cactcggggc ctcggctgga acgtagtctg cgcgagggcg taaagatgag
      481 cctgtcgact aaacctcgga gagaagaagc gtgaatcgag ccaacgcgac gtgaggtgac
      541 gaatgcaagg ttatccccga ctcgggaagg atgcgccagc tattcacatg acagcgtgcc
      601 agcatgaggg cacgtcgcaa ttcccacatg ctgggccgtg gttgctcgca tttgcggatg
      661 cctggcattc ggagccttcg acgagtacgt cgcacaccgc ttcagccttg gacgctccga
      721 gcgcgctctt ccagctgcgg acgccaggaa tgatggcggc cctcgatcag ctgggcacct
      781 ctctgctcgt gattgcgggt ccggaggtgt ggcagttgca gcggggttgc tatcagacct
      841 cccggcccgc gcccgaggag ctgcgcgcgg catcgagggc ctatgccgtt gattgcaacc
      901 cgccaaccgg ggccttcttt ttggtggacg gcaacggaac gctacgattc gctcactaca
      961 cgagcctcgg aaagacaccg gcggcttcgg tgctgactga agccgtgctg tcggcttggc
     1021 gagagttctc gggccgaccc gggacgggtg cggagcggaa tgagcgcagg atctcgtgca
     1081 tcgcttcgga gctgatgcac gccctagtgg cggcgccgtc gcccgctgag cctcgccaca
     1141 ccgggacctt tgcgagcact agcgccgcgg cactgagttc gtagccgcca gcgcgctcgg
     1201 ctcgcgacgg gaggcaagag ggctcctcga gggggccgcc cacatgcccg ctcgcctcat
     1261 ggttcgagct ctgcaagaca gcgaccggaa ctcggactca gttgagccga gactgcgcac
     1321 gcacgtcggg cgccgagagc gcgctcagca ggttgcgcag cttttcgacc agtagccacg
     1381 atcgctggct gggatcgagc tcttccaaga accgctgacg ctcgtcggga tcggcgacta
     1441 cagcagcagc cagcatatcg attcgagcgc cggcgctcgg cacctcgtag gcccgctcca
     1501 acaagccccg cacggactcg ggcaagtagg gacgcagctt ctcccacagt cccgcgagct
     1561 cccgctgcca agagaggagc gcataagcgt cgctcggagt ctgatcgggt aacagctcga
     1621 cgctcacgac gcgataggcg cggctggtcg ggagctcccg cgtgacccgc actcgtgaaa
     1681 gcccctgcac cacgagatca tagcgcccgt ccgggcgagc ctcatcttgt acgactcgac
     1741 cgatcccgca caccggatga aacgcgggtt tgccttcata gtccgcctcg tagccttcgc
     1801 ataggcgcgc cacgacgatc agacggtcac ccgctaggat atcctccgtc atctgccgat
     1861 aacgcggttc gaagacgtgc agcggtaggg acgcgccagg aaatagcgcc acgttgggca
     1921 gcgggaacac cggcacgcgg gctaggtccc gagcggacaa gcggctggaa gacgagctca
     1981 tgacgcgctg actatagctt tgggatggcg gccgcggcag cctcttggtc tgggtccgcg
     2041 ggtggcgtgc tgagagtacg ccgtttttcc ctgaccaggg ccgtgagcgc ctcgcgcgtt
     2101 acgcccagcc gctctgcagt ttttccggca agtgacgttc gcgccacgcc ggtcacgaag
     2161 cggtaggtcg gctcgctgcg cgcatccagt tcgacctgca gaaactcgag atttcgaacc
     2221 ggccggtcgc gcgcgaggcg cgccgcaaac tccaggaaat gtgtcgtgat gaaggcctgt
     2281 ggctcgagct cgcacagcaa gctcaccacc agctcgaaaa tgccttcacc ttcggacgga
     2341 ttcgttccgg agcagagctc atccaaaata accaaactgt tgacgtccag ctcttcgaag
     2401 gtgtggcgaa tccggcgcag ctccgtgccg aggcgacctt cctgatgctc ggcactcgcg
     2461 acttggatga gtgaaacgaa caagccatcg cgaaacgcca gccgcgcgcg cgccgcgggc
     2521 acgaacatgc cggcttgtcc aagcagctgg gaaagcccaa gggcctcgag caggcgtgtt
     2581 ttaccgcccg agttcgggcc ggtaatgatc gtcagcgcgc tcggcccgac ctcgagcgtg
     2641 catgggatcg gcgccttccc ctccaacagg agcaagggat tgaacaaagc ctccagacga
     2701 gtctcgctat cggcgtggcc acgttggacg atctcgggca ggcacatctg gagcccgagc
     2761 tctgcagcgc gcgccttgag cgcgagcgca gccaagtaga actcgaaatg cagaccgatc
     2821 tggaacaggc cgattacggc gctctgcaca ccgtcgaaca cctgattgat gagtcgcccc
     2881 agaatctccg cctcgcgcac gcgatagccg cggaagaaaa agcgcaagcg cgtcaagaaa
     2941 cgctgtagag gcgcgacatg aaatgggttt tcgcgattct cgcgcgcgga ggtgatctcg
     3001 aagccacgta acccgccgtc atgagcgagc cgcacccgca gctccaccgt ggccaggtga
     3061 ctctcatgat cgagtacagc cagcaaatct cgatagctct tgctgtcccg cacctgagtc
     3121 gcgaattggg cggcgcgcgc caggccggag gtggcgtgcg aaaagccgtc gtgcaacagc
     3181 tcgatggcgt gcttcacgtc gcgtaggata tccacgcggc gcgcgatcac gtcgtaacgc
     3241 ttgccgcgtt cgcccgcctc gaggcgagag atgagctctt gaatttgcag ccagctgcgc
     3301 tccatcgcgg tgcgcaactc gggccgttgc tccagctccc gcagtacccc ttgtcggaac
     3361 accaccgtgt ccaaatcaat gggcggctgg caaaggcaac ggatcagcaa gccccggtga
     3421 tagttgcgct cgcgtttctt gacgcgaatc accatgcact tctgcaccac gtcgctgacg
     3481 aagaggtccc gcgcgaacga gtccgaccga aaccgtgaag gggcgagttt ggcggcgttc
     3541 aacagacgat cgaaatcttc ccccgtactg cccgcggcaa atgcaaacgt gagcgcctgt
     3601 tggagggccg cttcgtcgag ccacaatcgc ggctcggggt gcagcagatc ggcagtttgg
     3661 gaggccatgc cggagctagc gctccttctt tcctactcga tccggctggc gaatggttca
     3721 caaaccgcta gcgcgcggtc aagcaaacct tccttttgca tgactatgcc tggcgctcgg
     3781 gccgatccac tagagtagcc cgctcgacat gcaggttgta cgggactaat ctgcgccgcg
     3841 aaagctcgag cctgttaccc cactcagccg tgcactcagc cccgtgacga cagccactcc
     3901 cattcctccg ccccctccct caacagactc gcaagctcgg agcgagccgt cctctttcgc
     3961 ggccacagca ggcaagagcc tggaacccgt aggaggcgag agcccggcag ggaagacgtc
     4021 cgccaccgtg ccgcctagag tgcaccgtgc aggtgctctg cgccggatgt ggcactggtt
     4081 ctggcagggc cctcgagcac gtgagctgag agaacagcgc gcgtccacgc gagtcctcga
     4141 gctttctcgc cgcgctcgcg tcgccctgga gttggccacg cacgccgaag atcccaggga
     4201 gccgctgcga gctggtgacg ccaagccgct cgcggcggag ctccgaagag aagccgccta
     4261 ctgggcgctg cgcgcctacg cggaaaatca aggggccgtc ctggcggaca acgacgggct
     4321 gaacgcagtc gccaagctcg ttccccaaga gctgttggac tcggccttcg aagcagacgc
     4381 agcggttcaa ttaagtgagc tcgagctgac accctttcaa cgcctttcgg acttggatcc
     4441 ggtcgtgatg gctcgttttt cggaccgggc cgaacgtttc acgcggattc tgctgaacgt
     4501 gaccgacgcg ccggcgatcg agctcgagcg actctggctc agacgtttgc tccgcgtcgg
     4561 ggctatcggc gcgttgttgg ggttgaccgc actgacggcc atcaagctgc gcgactggca
     4621 agaggatgct cgggatctgg caaagggacg ggcgtatcgg gtcagctcgg gtgcgctcgg
     4681 ctgtcgaagt ccggagcagg agtgtagcga aagcccattc ttctttttcc acacggcgga
     4741 agaagacaaa ccttgggtcg agatcgatct cggattggtg cgcacggtat ccggagttcg
     4801 cctcgtcaac cgtaccgatt gctgcacgga acgcggcacc cccttggtga tcgagctgag
     4861 cgtagaccaa cagaacttcc gcgaagtcgc caaacaaacg aacgagtttc gctcggtgaa
     4921 attggagttt ccgacgagca gagcgagatt cgtgcgcgtg cgcgcgctca atcgaacgat
     4981 actccacctc caacgggtgc gagtattgcc ataagctggg gcggtctcgt cagttcagcg
     5041 gcacgctcgc attcaacgac ttcgagtaac cgcgccgagc tacgacccaa ggcgctctga
     5101 ctgccgctac gccgaagtct ttagccaacg cggtgcttgc ctgttcgc
//
LOCUS       DASTKU010000922        18915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_848696, whole genome shotgun sequence.
ACCESSION   DASTKU010000922 DASTKU010000000
VERSION     DASTKU010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 18915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18915
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848696"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFQ61_32455"
     CDS             complement(<1..303)
                     /locus_tag="VFQ61_32455"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET9959262.1"
                     /translation="MGTLVHLVSYSSERSDAAQVELAFDAALREMDRLELLLSEWRTD
                     SEVGRINQGAGQWVAVGAETLQLLQRAAWASKISGGAFDVSFQVLSSLWKFGSAQSE"
     gene            622..1794
                     /locus_tag="VFQ61_32460"
     CDS             622..1794
                     /locus_tag="VFQ61_32460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET9959263.1"
                     /translation="MTLDRRALDQAVEDAYPKLVELARNLHENPELAYQEHRACSWIR
                     ELLESHGHAVQSRAGGVDTAFITRAAGRPGPRIAILAEYDALPEIGHACGHNLIAAGA
                     IGAFLALARYVPELNGSIELIGTPAEEGGGGKVRLLEAGVFQGLDAAMMYHPYDRDIL
                     AHPSLATVRLSMTFRGSPAHAAIAPWDGQSALTACLETFRLIDSQRVHFRDGVRVHGI
                     ITDGGKAVNVIPERAAALFLLRAPDLEELTRIRQIAERCARGAALAAGVEVELTAKPG
                     YKNMQNNLTLARRFGAALQALGRKPRETDPSVGAGSTDMGDVSHALPAIHPYLGICDA
                     GETICHEHAFAACAISERGLDTMRTAAKALARTAVDVLEDASLLREIQREFEQTKL"
     gene            1871..2653
                     /locus_tag="VFQ61_32465"
     CDS             1871..2653
                     /locus_tag="VFQ61_32465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959264.1"
                     /translation="MMRSRYASLCTLVLVAACGGAAANSSGTMSPEAESQPSSGEGNS
                     DPAPAAEGSNPAGTAEASKRAEESAKAVPSSNASSESSANKPSEGGTEPEVVSEPARN
                     VKYVVSPEGLRVEVEGVSFSPKGEAVKTAGGYTIRFKVEARAKDGKTHSLLAPESKEL
                     AFAGRVKGPDGEATRFGDKREGDRALTLGEKPVTLKRSYPGPGEKPLSAGSELELMVG
                     LWGIGADESSRRPLNKFFRATVKLDKEKPRVVIAPPEGTSKE"
     gene            complement(2715..3179)
                     /locus_tag="VFQ61_32470"
     CDS             complement(2715..3179)
                     /locus_tag="VFQ61_32470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HET9959265.1"
                     /translation="MTSSEKPVANASPTAPVPAPASKFSAENTPFSRIGGEDAVRALV
                     ERFYDVMDQHEPELAALHALENGRVSRRARDRFASFLIGWLGGPDEYVQQNGHPRLRM
                     RHAHLPVSGAMRDAWVACMRRALDHRDVDPELRSFLELRFVELAEHLRNMPG"
     gene            3379..4719
                     /locus_tag="VFQ61_32475"
     CDS             3379..4719
                     /locus_tag="VFQ61_32475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HET9959266.1"
                     /translation="MTLSDNDSFSSARPSSQLELADDSVAAEPVAADSGLSRIFRAAR
                     MLVINPEFRVILVCNVLMGLGSSFVMPFQSMWGTLEVGMSLRLFGVFMTVTAIANMAI
                     STVVSQRSDTRYSRRSVLLTGAASAALGYLGYAVIRELWVLFLIGSLLLGLASVTFSQ
                     LFAYARELVNRSSIPPSEAPLYMSTFRMCFALSWTVGPAIASMALAHLSYVGLFGGAA
                     LLYLALYLMVLWRIPKSAAPERPPVEGVAPPIVPMSQLLKVDMVLSWFIALALLFTAN
                     TISMGNMSLYVMKVLGGTERHVGIIFSLAPVFEIPLMLYFGLLATRTDSAKLIRISAL
                     LAVLYYFAVSLVHAPWQIYPLQILSAAIVSVTSGVAISFFQNMLPHQSGAATNLYVNA
                     MRVGSTSGYLLFGTLAARFGHRGTYSACAALSLASLFMMFIWGRSRSRLGFART"
     gene            complement(4764..5618)
                     /locus_tag="VFQ61_32480"
     CDS             complement(4764..5618)
                     /locus_tag="VFQ61_32480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959267.1"
                     /translation="MNRRRGSRAACGLALLLGGARTAHATEPCVGELGARASAPASIG
                     VGPAGLGTVPEACPGRDVRLRAGGSVLVARADFYGLLQTTLEMGLRYAFGRNDWFSLS
                     VPGLQYRFAANATIEASSTSLSAGAFGYHHSFRLGPGLRFSPWLRVLIPTETNYRHAR
                     RYAVEQGFSVLGALSPRVSWMFGLAFPVYVTLIGGADHYVMTQAYVSELAYQPWPVFS
                     AAAGAGLRIRSGERRGFEAFDPRLCLRLHVSSVWHAELAAAAPLWGTDRTDLLASFGL
                     YAAFDRDP"
     gene            complement(5639..8614)
                     /locus_tag="VFQ61_32485"
     CDS             complement(5639..8614)
                     /locus_tag="VFQ61_32485"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HET9959268.1"
                     /translation="MRAAICRGYAGGVASACGVCATLTLVSCAVLSCTSATEGCLGGD
                     DPACLPASPCPALEFSCPSDTELVLRRVERPEDRPSGIKADAAPGDILLQNGSVSVVL
                     AGLDAPRGLAPSGGAIVDLAPREGAAEDAIGHVFQAVGILPDDAAHYDAVELLDQRPS
                     SVSAIFRGRLDARPDMRIATRYELRACDPGLRIRSELFNGARDALPVFLSDAAFWGDR
                     NLTPFVPVPGRGYVHPELDLEHLDRAFGDTPFLVADAHDEASSTYGWLACDARQLSGF
                     HNTTISAVGPERVLLEPFDGLVFERFAAARPGPGLSRAVDTVLAARRMLFGEPTTRVT
                     GKVRAAGAIGDPFAAGERRLSLLFFDSEKQTAVSEIVPNPAGEFELSLPRGRHYRVQP
                     FVLGRAFGAGVEFEADVDVIALNELESPTPGRLHVEVNDAEGEPIVAEVVLIPAAPTS
                     ASQVQGSVFGVFDEKRCTPYLGPPHGGSPACNRVLVDATGRTEFWVPPGNYYVYATHG
                     PFWSLARQRVSLRASERQQLSFVLRSLPLLPVDVLSADLHVHAGASFDSNLPERDRAL
                     SFIAQGVQVIAATDHDVVTDYASTLRELGLSDRVRVMPGVETTGQILFLRPPGSEIPR
                     VIGHFNFWPLRRDIGLPRNGAPDDERLEPGALMDRMQPLFSGVSVAQMNHPFARGELG
                     RDHGYLSAIGYDPRRAVPPGPNDTPEGQLLRASASGRTNLAFDAQEVMNGASLENFLR
                     YRAGWFSFLNQGILRAGTANSDSHTLAIEVLGYPRNLVLGQPELRAFDPDRFNEAVRR
                     GALIGTNGPVLEVCLKESDGDCLPPSLEARVPSAASKLSVRVSAAPFIPVSEVRFIVN
                     GQQVKTVALPEALPSEPFGVEGLERYRAELDLRELVASSGDAWLVVEAGLPLPLSADL
                     EDDDGLPDTTDNDRDGRVDAKDGIGDFREPARAAPGGAEQSDMRIHVEALAPGVWPTS
                     FSNPIIIDWSGDGFEAPGL"
     gene            complement(8620..9729)
                     /locus_tag="VFQ61_32490"
     CDS             complement(8620..9729)
                     /locus_tag="VFQ61_32490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HET9959269.1"
                     /translation="MALLLSSLIVLLSLGAAVAIEWRSPSQLARGAGLLLWVIGGAVG
                     YATAQLERIVLQATGLDFEVRVAGAGGALLAAFLLSGPLEEAAKLAIVWPLYRGRRLR
                     TAFDGIRLATFVALGFAAAESVLQVQAASAPGLAALRALLSLVAHVFFAGAWGYSLGA
                     GRPQGRWFSWVWFAALLLHALYDHIVWGRGPGYLAAALPMLGFMAIGAYWVLRSEPVV
                     HSHGLQHDPEALLRMQRALLSHDRGIAVSWVVMGVFVTLGLLLTLSVLSVFVGHRLGV
                     DFTLADEADVRSAAPLALLGTGLLLAFPLAGFLISRASAATGVLEAALSALIALSALI
                     VLLALTAPVAVLFALAVTPIAIGLTCGGAWVGLDR"
     gene            complement(9757..10545)
                     /locus_tag="VFQ61_32495"
     CDS             complement(9757..10545)
                     /locus_tag="VFQ61_32495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959270.1"
                     /translation="MKPCWCLLVAAVVAYPSFAFAQAQTEPGALAPPPAVQPVAPEDA
                     PTPTEQELSRADREDAGRGLEFVWLNGEVGFQHLGLQTFHANSLVDAQIVKSSQSGMV
                     FGAGAGVRLIFLTLGGRFRLGTFDAWQLWTLNAELGFRIPIGALEPYFTFGGGYASLG
                     SFDSKSIGTSLGDFGVSAGGLNVRGFNVRGGIGFDYYVARVLSIGAQASGDVLFLSRP
                     KSKVVATQATGTGAEAAAAAQVYARDGSAIGGDVTLTAMVGLHF"
     gene            10650..11081
                     /locus_tag="VFQ61_32500"
     CDS             10650..11081
                     /locus_tag="VFQ61_32500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959271.1"
                     /translation="MLMFGNFKTLAALASLTVIAASCTLITDVDRSKIKESGGTTGSG
                     GTTSSGGNTAVTGGNTAEGGSTAEGGATSGSGGAEGGSGGDTSAGSGGQAGAGAGGTD
                     SGGAGAGSGGASGGEAGAGSGGDVAASGGDTAQGGATGPAT"
     gene            complement(11198..12802)
                     /locus_tag="VFQ61_32505"
     CDS             complement(11198..12802)
                     /locus_tag="VFQ61_32505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959272.1"
                     /translation="MFLFGRRGGARLSFLTGLSCVATVVFVLCVLAERPAHAYPWMIR
                     HSYTTCNTCHADPSGGELLTLYGHAISYEVLSTKWGGRSEASASRRLREEREVAAAIS
                     RAAHQKNAPKKKVAAEDVDLEGEGKSKTKAAAADANTAEANTAEGEAAAGDTEESAVE
                     EGSAEEGAAEGGKAEAGKEEAAEASTASDASGSEAASSEPSPFAGMGIAGPLFGLFPA
                     KDQLLVGGSIRLASIYRLQGDDRNRFFPMQADLYGQVRLGSHFRVGASLGVAKVPVGS
                     PHARAAQITGNQGDGYNAISRTHWIGYDFGEEGQHTIRAGRLNLPFGIRMSEHTMWVR
                     ERTQTDRESDQQHGVSLAMGFEKARFELMGILGNYQIHPDKFRQRGYAGFFEYFVAER
                     LTMGVSSLYTTASADALYLEDQKTARQAHGLFVRSAPSEAVAIMAEFDMLSRSRRELG
                     YAGLLQVDTELVSGLHFILSGELADSGYPKNGGPAALPRTAGAGKPALGGWIGAQWFF
                     LPHFDFRVDAIIREQKQILGQFHVYL"
     gene            complement(13037..13561)
                     /locus_tag="VFQ61_32510"
     CDS             complement(13037..13561)
                     /locus_tag="VFQ61_32510"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9959273.1"
                     /translation="MFRRTAFALLLSATTFTLAAHAAMSGGGPLKISGKAVGLSFTGT
                     AKFSVSENEGKLVFVTNLRDSLDMGVRDDHTKRSFKVKEFPKATLTIDKSKLKLPEKG
                     ESSGSVTGHLTLAKGAKDVNVNYTVAKEGDGYRIKNASFTFDYTKFGVEEICKVICVK
                     PEVNVSVSDVKING"
     gene            complement(14019..14657)
                     /locus_tag="VFQ61_32515"
     CDS             complement(14019..14657)
                     /locus_tag="VFQ61_32515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HET9959274.1"
                     /translation="MPDFNHLIKRALVSLELDPDLPSGRSTRLGLASTWATRVFEWNQ
                     RIDLTAARGPEELVDLLIADAAIVANNCSQDSESWLDVGTGAGAPGLGLAVLLPALRI
                     QLLEPKAKRIAFLRTVVGELGIPVRVLRGRSEEQPNGACSVAISRATLPPPQWLEEGA
                     RLARHSVWVLLAQADAPALQGWQEAQRLEYRWPLTGVERTALRFERVESEPR"
     gene            14783..15841
                     /locus_tag="VFQ61_32520"
     CDS             14783..15841
                     /locus_tag="VFQ61_32520"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET9959275.1"
                     /translation="MPAADFPLHTLEPLVRLATGVEPPLEFSEIFGGASVRRFFRVKA
                     GPGSSLIAMYVPAQSHELSKPPSKPRRWPFLEVRELLEERGVRVPKLLAEDCANGWIL
                     VEDLGDTLAQHLNKHPEERDRLYSAAVRSLSLAQRALRELPEHSVILERAFDEDLLTW
                     ELHHFREFGLEARGISLNPRQAEVFERAVAHIASTIAAWPRGFVHRDYQSRNIMVVEP
                     HQPELGWIDFQDAMLGPRVYDMVALLNDSYQNFDVAFVSTRLDEFGAALGLAALEREA
                     LKREFAWVTVQRKLKDAGRFIFIERRHDNPSFLPFFEPTLRKAYAALRSLNDGCLGDL
                     GDLLVELFPMLNDLDVRR"
     gene            complement(15892..16983)
                     /locus_tag="VFQ61_32525"
     CDS             complement(15892..16983)
                     /locus_tag="VFQ61_32525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959276.1"
                     /translation="MRIRFGQRNEAEQRRLHEIEERAARMLGEGVLGAICPELWLEHS
                     GNGALALRPREAALLPLVDHLVTQLGSKAPPIALAPVLPLEEALKRAREIADFSQARL
                     RVGFARGHLLDVVFALPGGSGSEREIEAATRLVFDLLGPRLALAWLGRVSVVPAPRLG
                     PLKVLDSGAPSGNLVVAELAPTFKAAIQGLYAGLPDSPYGESSHQEEWSLFELEPEPA
                     RDYAAQDDLVFASTCVPEALKCYLSGLSFASERFSRFGERFFYIKYRCRGAPEVRLEE
                     RQQLEDLLDRSLLVAKAGRVVGAGLGLAYSYVHCALSSLERGLALMAEAAQRRELPEQ
                     AWLLPFDAEFAEEWLGVWPGAEAPPVHRA"
     gene            complement(16985..17506)
                     /locus_tag="VFQ61_32530"
     CDS             complement(16985..17506)
                     /locus_tag="VFQ61_32530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET9959277.1"
                     /translation="MAPARPVEPPERVVPARPLVAPSWAEPQANDGRMLSEQFRNARA
                     LQHFRGRVSYYSDSLSGRPTASGEPYQPARFTAAHRSLPFGSVVRVVREDTGEEVYVR
                     INDRGPFVRGRILDLSGAAARQLGLLRRGVMPVRADVVLYGERRDASKPGGKKRKNKA
                     RPRRRPSSIAERN"
     gene            17779..>18915
                     /locus_tag="VFQ61_32535"
     CDS             17779..>18915
                     /locus_tag="VFQ61_32535"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET9959278.1"
                     /translation="MTPRVARSKPDRVRLTRSGLSLGDTNLPLLVGSVHYWRLDPDVW
                     RDALEAVKALNLKVVDTYVPWSVHERSPGHYEFGENERHLDLVHFLQLAHELGLYACV
                     RPGPHVNAELTQFGIPERIIWDPQCQALSARGARVVLPVPPLAFPVPSYASRTFLREA
                     SVWLSAVGDRIAPLCYPRGPVVLCQVDNEGAMYFRDGVYDQDYHPDAIRGYHKFLKRK
                     YGTPAALSQALGRNVESFEIEPPRGLEANTSRELTPHLDWAESQEELLSHSFGKMREA
                     LEEAGVDVPFSHNFPIGETLTPLDPKRVQKVVDLVGLDYYHAASPTQRQEIARRTSDL
                     AARSELEHVPAFACEIGAGFPPFFPPLTETDNTFTLLTALAYGLK"
BASE COUNT         3592 a         6141 c         5908 g         3274 t
ORIGIN      
        1 ttcactctgc gcgctaccga acttccagag cgaactcaga acctgaaaac tcacgtcgaa
       61 ggcaccgccg gagattttgg aagcccaagc cgcgcgctgc agcagttgca gagtctccgc
      121 gccgacggcc acccactgac cggcgccctg attgatgcgc ccgacttcac tatccgtgcg
      181 ccactcgctc agcagtaatt ccaaacggtc catctcgcgc agggcggcgt cgaatgcgag
      241 ctcgacctgc gcggcatcgc tgcgttcgct actgtaagag acgaggtgca cgagcgtacc
      301 catggccggc tgcttgcgct cgatcttgcg tggttgaggc agcgcggacg ggattgcgag
      361 gctggagggc ttcgctggtg gggccgaacg gggctccgcc ggttgcgtca aggcgcggtt
      421 ttgggtttca tggcgcgcac acgcgcaagc caacgcgaat gcgccaacca cgccacaaag
      481 ccgggttatt ccaagctttt tcctgcgcgg cggggtaccc gcactcggtt ctgccccggg
      541 ctgcgcgcag cctgtctcgg cgagggagag attggcaaga actggctgtg ggaagcgcat
      601 ctcgggctat gctaccgcgc aatgacgctg gatcgtcgag cgctggatca ggccgtcgaa
      661 gatgcttacc cgaagctcgt ggagctcgcc cgcaatcttc acgaaaaccc cgagctcgct
      721 taccaagagc atcgcgcgtg ttcgtggatt cgcgagcttt tggagagcca cggccatgcg
      781 gttcagagcc gggccggggg cgtagacacg gccttcatca cgcgagctgc cggccgcccg
      841 ggcccgcgga ttgcgatttt ggccgagtac gacgcactac ccgagatcgg ccacgcttgt
      901 ggtcacaact tgatcgcggc cggcgccatc ggggcgttcc tcgccctcgc tcgctatgtt
      961 ccggagctga atggcagcat cgagttgatc ggaacgcccg cggaagaggg cgggggcgga
     1021 aaggtgcggc tgctcgaggc cggagtcttc caaggcctcg atgcggcaat gatgtatcac
     1081 ccctatgatc gcgacattct cgcccatcca tcgctggcca cggttcgact cagcatgacc
     1141 ttccgaggca gccccgcgca cgcggcgatc gcgccgtggg acgggcaaag cgctcttacg
     1201 gcgtgcctcg agacattccg cttgatcgac tcgcagcgcg tgcattttag ggacggagtg
     1261 cgggtccacg gaatcatcac ggatggtggc aaggccgtga acgtgatccc cgaaagagcc
     1321 gccgccttgt tcctgttgcg agccccggat ctggaagagc tcactcgcat ccgtcaaatc
     1381 gcggagcgct gcgctcgcgg cgctgcgctc gcggcggggg tggaggtgga gctcacggcc
     1441 aagcccggtt acaagaacat gcagaacaat ctcaccttgg cgcgacgctt cggagccgca
     1501 ctgcaagctc tgggtcggaa gccacgtgag acggacccca gcgtgggtgc ggggagtact
     1561 gacatgggcg acgtcagcca cgccctacct gcaattcatc cttatctcgg catctgcgat
     1621 gcgggagaaa ctatctgtca cgagcacgct ttcgcggcct gcgcgatcag cgagcgtggc
     1681 ctggatacca tgcgcacggc cgccaaggcc ttggcccgaa cggccgtgga cgtgctggag
     1741 gatgcctccc tactccgaga gatccagcgc gagttcgaac aaaccaagct ctgagccgcg
     1801 actgcctcga gggcgttttt cacgcgcggc accgcgcgcg atccggcgtg aatcggatat
     1861 agtctcggcc atgatgcgtt caaggtacgc ctcgctgtgc acgctcgtcc tcgtcgctgc
     1921 ctgcggtggc gcagccgcaa actcgagcgg cacgatgtcc cccgaagccg agagccagcc
     1981 gagctcggga gagggcaact cagatcccgc cccggccgcg gaaggctcga acccggccgg
     2041 aaccgcagaa gcttcgaaac gtgccgaaga gagcgccaaa gcggtcccga gcagcaacgc
     2101 gagctccgaa agctcggcga acaagccgtc ggagggcggc accgaacccg aggtcgtaag
     2161 tgagcccgct cgcaacgtaa agtacgtggt cagccccgag ggcttgcgcg tcgaggtcga
     2221 gggcgtgagc ttctctccca aaggagaggc cgtcaaaact gcgggtggat acacgattcg
     2281 tttcaaagtc gaggcgcgcg ccaaagacgg aaaaacgcac tcgttactcg ctccggaatc
     2341 caaggaattg gctttcgcgg gccgggtgaa ggggcctgac ggagaagcca cccggttcgg
     2401 ggacaagcgg gaaggagacc gcgcgctcac gctcggtgag aagcccgtca ccttgaagcg
     2461 gagctatccc ggccccggcg agaagccgct gagcgcaggc tcggagctcg aactgatggt
     2521 ggggctgtgg ggtatcggcg ccgacgagag ctcgcgacgc cccctgaaca agttcttccg
     2581 ggccacggtc aagctcgaca aggagaagcc gcgtgtggtg atcgctcctc cggagggtac
     2641 cagcaaggag tgataatcga gcaagcgcgc tcgagttcaa cggggcctca cgctcacgtg
     2701 agcggcgcgg gggttcagcc aggcatgttg cgcaaatgct cggcgagctc gacgaatcgg
     2761 agttcgagaa agctcctcaa ctccggatcg acatcgcggt ggtcgagcgc tcgacgcatg
     2821 caggcgaccc aggcgtcacg catcgcgccg ctcacgggaa gatgagcgtg ccgcatgcgc
     2881 aaacggggat gcccgttctg ttgaacgtat tcgtcaggtc ccccgagcca gccgatcaag
     2941 aacgacgcaa atcgatcccg ggcgcgccga ctcaccctac cgttctcgag ggcatgcaag
     3001 gcggccagct cgggctcatg ctggtccatc acgtcataga agcgttcgac gagggcacgc
     3061 accgcgtctt caccgccgat gcgtgaaaat ggcgtattct cggcggaaaa cttgctggct
     3121 ggggcaggaa cgggagccgt agggcttgca ttggcgacgg gcttttcgga cgacgtcatg
     3181 gcttcgataa tcgaaagcgt gttcttagca tcccgcggct gatttccagc agatccaacg
     3241 cttgcggtta gattcctggc gatttgaccg ttgaagccgc cgttgtttcg ctcagaactt
     3301 gcggatctca atccatcccg acgactgacg ccattcccgc tgcactgcaa accgcgatca
     3361 gacgcttcgg gctcctccat gacgctctca gacaacgatt cgtttagctc ggcccgaccg
     3421 agctcgcagc tcgagctggc cgatgactcg gtcgctgcgg agccggtagc tgccgactcc
     3481 ggattgagcc gcatcttccg cgcggctcgc atgctggtca tcaacccgga gttccgggtg
     3541 atcttggtct gcaacgtact gatgggcctc ggctcgtcct tcgtgatgcc cttccaatcc
     3601 atgtggggca cgctcgaagt cggaatgagc ctacgcctgt tcggcgtgtt catgaccgta
     3661 accgcgattg ccaacatggc catcagcacg gtcgtgtcgc agcgctcgga taccagatac
     3721 tcccgccgca gtgtgttgct gaccggggcc gctagcgcgg cgctaggcta cttggggtac
     3781 gcggtcatcc gagagctctg ggtgctgttc ctgattggct cgctgctgct cggcctcgcc
     3841 tcggttacct tctcgcagct gttcgcgtac gcgcgcgagc tcgtcaatcg ctcgagcatc
     3901 ccgccatccg aagcgccgct gtacatgagc acgttccgca tgtgcttcgc gttatcgtgg
     3961 accgtcggac cggcgatcgc ttcgatggcg ctcgctcacc tctcctacgt gggtttgttc
     4021 gggggcgccg cgctcctcta cttggcgctg tatctcatgg tgctgtggcg aatccccaaa
     4081 tccgccgcgc ccgagcggcc cccagtcgaa ggcgtcgcgc cgccgatcgt gcccatgagt
     4141 cagctcctga aggtggacat ggtgttgtcc tggttcatcg cgctcgcgtt gttgttcacg
     4201 gccaacacga tctcgatggg caacatgtct ctttacgtca tgaaggtgct cggcggcaca
     4261 gaacgacacg tcggaatcat cttcagcctg gcgccagtgt tcgaaattcc cctgatgctc
     4321 tactttggcc tgctcgcgac gagaaccgac tcggccaagt tgattcgaat ctccgcgctg
     4381 ttggccgtgc tctactactt cgcagttagc ttggtgcacg cgccgtggca gatttatccg
     4441 ctgcaaatct tgagcgcggc gatcgtctcg gtgacttcag gagtggcgat tagcttcttc
     4501 caaaacatgt tgccgcatca atcgggggcc gcgacgaacc tctatgtcaa cgcgatgcgc
     4561 gtcggctcga catcgggcta tctgttgttc ggaacgctcg cggcccggtt tggccaccgc
     4621 ggtacgtaca gcgcttgtgc tgcgctcagc ctcgcctcgt tgttcatgat gttcatttgg
     4681 ggtcggtccc ggtctcggct cgggttcgct cgaacctgaa actcatcccg atcggagcta
     4741 gtcggtgcga cgtcgggtgc gaatcatggg tctcgatcga aggctgcgta caatccaaag
     4801 ctggccaaga gatcggtgcg atcggtcccc cagagcggcg cggccgccgc gagctcggcg
     4861 tgccaaacgc tagaaacatg cagccgcaag cagagcctcg gatcgaaagc ttcgaagccg
     4921 cgccgctcgc cggagcgtat ccgcaggccc gcgccggcgg ccgcggagaa gaccggccaa
     4981 ggttggtagg cgagctcact cacgtaggct tgggtcatga cgtagtgatc cgcgccgcca
     5041 atcagcgtca cgtatacggg gaaggctagc ccgaacatcc agctcactcg cggagacagc
     5101 gcgcccaaaa ccgagaagcc ttgctccacc gcatagcggc gcgcatggcg atagttggtt
     5161 tccgtgggga tgagcacgcg aagccaaggt gagaacctca acccgggccc cagacggaag
     5221 gagtggtgat agccgaacgc tccggcgctc aggctcgtcg agctcgcctc gatcgtggcg
     5281 ttggccgcga agcgatactg gagccctggc acggaaagcg agaaccaatc atttcgaccg
     5341 aaggcatagc gaagccccat ttcgagtgtc gtttgaagca agccatagaa gtccgcgcgc
     5401 gcgaccagca ccgagccccc tgctctcaag cgcacgtccc ggccgggaca cgcttcaggc
     5461 accgtgccta gccccgcggg tccaacccca atcgatgccg gggcgctggc gcgcgcgccc
     5521 aattccccga cgcacggctc ggtggcatgt gcggttcggg cccccccgag cagcagcgct
     5581 agcccgcagg ctgcgcgcga gccgcgcctc cgattcacgc tcgagcggcg ccacgacctc
     5641 ataatcccgg cgcctcgaag ccgtcccccg accaatcgat gatgatcggg ttgctgaacg
     5701 aggttggcca tactccggga gccaacgcct cgacgtgaat tcgcatgtcg gactgctcgg
     5761 cgccgccggg tgcggctcgt gccggttcgc ggaaatcgcc gattccgtct ttggcatcga
     5821 cccgcccgtc gcgatcattg tccgtggtat cgggtaagcc gtcgtcgtct tccaaatcgg
     5881 cgctaagtgg caatggcaag cccgcctcca cgaccaacca ggcgtcaccc gagctggcca
     5941 cgagctctcg aagatcgagc tccgcgcgat agcgctcgag accttcgacc ccgaacggtt
     6001 cggacggcaa ggcctcaggc aaggccaccg tcttcacctg ctgcccgttc acgatgaaac
     6061 ggacctcgct caccggaatg aatggcgccg cggagacgcg cacggacaac ttgctcgctg
     6121 cgcttggaac gcgagcctcg agcgagggcg gcaggcagtc cccatccgac tccttcaaac
     6181 agacctcgag caccggtccg ttggtcccaa tcagcgcacc gcgccgcacg gcttcattga
     6241 accggtccgg gtcgaacgcc ctaagttctg gctgcccgag caccaagttg cgtgggtagc
     6301 ccagaacttc gatggcgagg gtgtgcgagt cggagttggc ggtgccggcg cgaagaattc
     6361 cctggttcaa gaagctgaac caaccagcgc gatagcgcag aaaattctcg agtgacgcgc
     6421 cgttcatgac ttcctgtgcg tcgaaggcca ggttcgttcg tccactcgcg gaggcccgga
     6481 gcagttgacc ttccggggta tcgttggggc cgggcggcac cgcgcgacgc gggtcgtagc
     6541 caatcgcgct caagtagccg tgatcgcgcc cgagctcgcc ccgtgcgaac ggatggttca
     6601 tctgggccac ggacacacca ctgaacagcg gttgcatgcg gtccatgagc gcccccggct
     6661 cgagtcgttc gtcgtccggc gcaccgttgc gcggcaggcc gatatcccgc cgcaacggcc
     6721 agaaattgaa atggccgatg acccgcggga tctcgctgcc gggcgggcgc aaaaacaaga
     6781 tctgccccgt ggtctcgacg ccgggcatga cgcgcacccg atcgctcaaa ccgagctcac
     6841 gcagggtgct cgcgtagtcg gtgacgacat cgtgatccgt cgcggcaatt acctgtacgc
     6901 cttgcgcgat gaagctcagc gcccggtctc gctctggcaa attgctgtcg aagctggcgc
     6961 ccgcgtgcac gtgcagatcg gcgctcaaca cgtcgaccgg cagcaacggg agcgatcgca
     7021 atacgaagct caactgctga cgttcgctcg cgcgtagaga cacgcgctga cgcgccagtg
     7081 accaaaacgg gccgtgcgtg gcataaacat agtagttgcc cggaggcacc cagaactccg
     7141 tgcgaccagt ggcatcgacc agcactcgat tgcaagccgg agagccgcca tgcgggggcc
     7201 caaggtaagg agtgcagcgt ttctcgtcga acacgccaaa cacagaaccc tggacctggg
     7261 aggctgaagt cggagccgcg ggaatcagga ccacctccgc gacgataggc tcaccctccg
     7321 cgtcgttcac ttcgacgtgg aggcgccctg gcgtggggct ttcgagctcg ttcaaggcga
     7381 taacgtcaac gtcggcttcg aactccactc ccgccccgaa tgcgcggccg agaacgaagg
     7441 gctgcacccg atagtgtcgc ccacgaggga gtgagagctc gaactctcct gccggattcg
     7501 gcacgatctc gcttaccgcg gtctgctttt cggaatcgaa gaagaggaga ctcaagcgcc
     7561 gctctccggc ggcgaaagga tcgccgatgg caccggctgc tcgcaccttg ccggtcacgc
     7621 gggtggtcgg ctccccgaac agcatgcgac gggctgccag cacggtgtcc acggctcggg
     7681 agagccctgg cccggggcga gccgctgcaa agcgctcgaa gacgagtccg tcgaagggct
     7741 cgagcaatac gcgctcgggc cccacggcgg agatcgtcgt gttgtgaaag cctgacagct
     7801 gacgcgcgtc gcaagccaac catccgtaag tcgagctcgc ctcgtcgtgg gcatctgcga
     7861 ccaagaacgg cgtatccccg aacgcccgat cgaggtgctc caaatcgagt tcgggatgca
     7921 cgtagccccg ccccggcact ggcacgaacg gcgtcaggtt gcggtcgccc caaaacgcag
     7981 catcgctgag gaacaccggc agagcgtcgc gcgcgccgtt gaacaactcg ctacgaatac
     8041 gcaggcccgg gtcgcaagcg cggagctcat agcgagtggc aatccgcata tcgggtcgcg
     8101 cgtcgagccg ccccctgaag atcgccgaca cactcgaggg ccgctgatcg agcaactcga
     8161 ccgcgtcgta atgcgcagca tcgtccggca gaatgccgac cgcctggaag acatggccga
     8221 tcgcgtcctc tgccgcgccc tcacgtggcg cgagatccac gatcgcgccg ccagacgggg
     8281 ctaagccgcg cggcgcatcg agcccggcga gcaccacact gacgctgccg ttctgcagta
     8341 ggatgtcgcc cggtgcggcg tcggccttga tccccgaagg acgatcctcg gggcgctcga
     8401 cgcggcgtag gacgagctct gtgtccgatg gacacgaaaa ctccagcgct gggcacgggc
     8461 tggcggggag acacgccgga tcatcgccac cgaggcaacc ctcggtcgcg ctcgtacaag
     8521 acagcaccgc gcacgatact agcgttaggg tcgcgcagac tccgcacgcc gaggcgacgc
     8581 cacccgcgta gcctctgcag attgcagctc gcatctgtat cagcgatcga gtccaaccca
     8641 ggcgccgcca caagtcaaac caatcgcgat cggtgtgacc gcgagtgcga acaacaccgc
     8701 cacgggcgcg gttagcgcca aaagcacgat caaggccgaa agcgcaatca acgcggatag
     8761 ggcggcctcg agcacgcccg tcgcagcgct ggcccgcgag atcaaaaatc ccgccaacgg
     8821 gaacgcgagc aacaggccgg tgccgagcag cgccaagggt gccgccgagc gcacgtctgc
     8881 ctcgtcggcc aaagtgaagt ccacgcccag gcgatgcccc acgaacacgc tgagcacgga
     8941 aagcgtcagc agcaagccga gcgtgacgaa cacccccatc acgacccagc tcacggcaat
     9001 accgcggtcg tggctcagga gagcgcgctg catgcgcagc aaggcctcag ggtcatgctg
     9061 gagcccgtgc gagtgcacca cgggctcgct gcgcagcacc caatacgcgc cgatcgccat
     9121 gaacccgagc atcggtaacg ccgcggccag ataaccgggc ccccgtcccc acacgatgtg
     9181 gtcgtagagc gcatgcaaca ggagcgccgc gaaccaaacc caggaaaacc aacgaccttg
     9241 agggcggcct gcgcccaacg aataacccca tgcgcccgcg aagaacacat gcgcaaccag
     9301 gctcaacaag gctcgcagag ccgctaagcc cggcgctgac gcggcctgca cttgaagcac
     9361 cgactcggcc gccgcgaaac ccagcgcgac gaaggtcgcc aagcggatgc catcgaaggc
     9421 cgtacgcagc cgccgcccgc ggtacaatgg ccagacgatc gccaacttgg ccgcctcctc
     9481 gagcggcccc gacaacagga atgccgcgag cagcgcgcct cctgcaccgg cgacgcgaac
     9541 ctcgaagtcg aggccggtgg cctgcagcac gatccgttcg agttgggcgg tcgcgtaacc
     9601 caccgccccg ccgatcaccc aaagtagcaa gcccgcaccg cgggccagtt ggctcggcga
     9661 gcgccactcg attgccaccg cagcgccgag cgagagcaga acgatcagag acgagagcag
     9721 tagagccatc ggcgcaattc gagtctttag ccaagctcag aaatgcaaac ccaccatcgc
     9781 ggtcagcgtc acgtccccgc cgatggccga cccatctcgc gcgtacacct gcgcagccgc
     9841 cgcagcttct gctccggtgc ccgtggcttg cgtcgctacg actttcgact tggggcgact
     9901 gagaaacagc acatcccccg acgcttgcgc gccgatcgag agcacgcgcg ccacgtagta
     9961 gtcgaacccg atgccaccac gcacgttgaa gccgcgtacg ttcaagccgc ccgcgctgac
    10021 accgaagtca ccgagtgatg ttccgatgct cttcgagtcg aacgaaccga gcgaggcgta
    10081 accgccgccg aaagtaaaat agggttcgag cgcgccgatc gggatgcgga aacccaactc
    10141 cgcgttcaac gtccacagct gccaagcgtc gaacgtcccg agccgaaaac gaccgcccaa
    10201 tgtcagaaaa atcagacgaa cgcccgcgcc ggcgccaaac accatgccgc tctggctcga
    10261 cttcacgatc tgagcgtcca ccaagctgtt cgcgtggaat gtctgcaggc caagatgctg
    10321 aaagccgacc tcgccattga gccacacgaa ctccaaaccg cgccccgcat cctcgcgatc
    10381 agcacgcgat agctcctgtt ccgtgggtgt tggggcatct tctggagcaa caggctggac
    10441 ggcaggcggt ggggccaggg cccccggttc ggtctgggct tgagcgaagg cgaaacttgg
    10501 ataagcgacc actgcagcca cgagcagaca ccagcagggc ttcatgccgc tcagggtagc
    10561 ggacttgctc ccccaaggca ttctcttgcg ttcggcccca aagctagagc aagattgccg
    10621 cccatcctac gaggggcccg gatccaccca tgctcatgtt tggcaacttc aagactctgg
    10681 cagctttggc ttcgctcacc gtcatcgcgg cgagctgcac cctgatcacc gacgtcgatc
    10741 ggagcaagat caaagagagc ggcggcacca ctggcagcgg cggcaccacc tcgagtggcg
    10801 gcaatacggc cgtcaccggc gggaatacgg ccgagggtgg cagcacggca gaaggcgggg
    10861 cgacctcggg ttcgggtggt gcggaaggtg ggtccggagg cgacacgagc gcgggctcgg
    10921 gaggccaggc cggcgctggc gcgggcggaa cggatagcgg aggcgctgga gcaggctcag
    10981 gcggcgctag cggaggagaa gccggcgcag gcagcggcgg agacgtggcc gcaagcggtg
    11041 gagacaccgc tcagggcggc gccaccggcc ccgctacttg aatcacctcg cggttcgagg
    11101 gcgctgaatc cgggggattc gggcgggcgc agaaccaggc gattagggga tcctcgaaac
    11161 gggatcccct aaagtcgttt tgtaggttcg gtgcagctca cagatagacg tggaactggc
    11221 cgaggatctg cttctgttcg cggatgatgg cgtcgacacg gaagtcgaag tgcggcagga
    11281 aaaaccactg cgcgccaatc caccctccca acgctggctt gcccgcaccg gcggtccgag
    11341 gcagcgcggc ggggccaccg tttttgggat aaccggagtc ggccagttca cccgaaagga
    11401 tgaagtgcag cccgctcacg agctccgtgt ccacctgcag caagcccgca tatccgagct
    11461 cgcgacgcga gcgggacagc atgtcgaact cggccataat cgcgaccgct tcgctcggag
    11521 cgctgcgtac gaacagaccg tgcgcctggc gcgccgtctt ttgatcttcg agataaagcg
    11581 cgtccgcgga agccgtcgtg tacaagctgc tcacccccat ggtcagccgc tccgccacga
    11641 agtactcgaa gaaacccgcg taaccccgct gacgaaactt gtctggatga atttgatagt
    11701 tgcccagaat ccccatcagc tcgaagcgtg ctttttcgaa gcccatcgcg agtgacacgc
    11761 cgtgttgctg atccgactct cgatccgttt gcgtgcgctc gcgaacccac atggtgtgtt
    11821 cggacatgcg aataccaaac ggcagattca gacgaccggc gcgaatcgtg tgctgcccct
    11881 cttcgccgaa gtcgtaccca atccagtgcg ttcgggagat ggcattgtag ccatcgccct
    11941 ggttacccgt aatttgagcg gcgcgggcat gcggagagcc cacgggaacc ttggcaacgc
    12001 ccaggctcgc gccgacacgg aaatgcgagc ctagccgcac ctgtccgtac aagtcagcct
    12061 gcattgggaa gaatcgattg cggtcgtcgc cctgcaagcg gtaaatagaa gcgaggcgga
    12121 tactgccccc aacgagcaat tgatccttcg ccggaaacag gccgaataga ggccctgcga
    12181 ttcccatccc tgcgaaaggt gaaggttccg agctagcagc ctcggaacca gatgcgtcgc
    12241 tggcggttga agcttccgcg gcttcttctt tgccggcttc ggcttttcca ccttccgcag
    12301 cgccttcctc ggcgctgcct tcttcgaccg cggactcttc ggtatctccc gcggctgcct
    12361 cgccctcggc ggtgtttgcc tcggccgtgt tcgcgtcggc agcggccgcc ttggttttgc
    12421 tcttgccctc accttccaag tcgacgtctt cggcggcaac tttcttcttc ggcgcgttct
    12481 tctgatgcgc ggcgcgcgaa atggcggctg cgacctcgcg ttcctcacga aggcggcgcg
    12541 acgcgctcgc ttcggagcgg ccgccccact tggtgctcag cacttcatac gaaattgcgt
    12601 gcccgtacag ggtcagcagt tcaccgccgg atgggtcggc gtgacacgtg ttgcacgtgg
    12661 tgtaactgtg ccggatcatc cacggataag cgtgcgccgg gcgctccgcc aaaacgcaca
    12721 acacgaatac gaccgttgcg acgcacgaaa ggcctgtcaa aaagctgagc cgcgcgccac
    12781 cgcgccgacc gaatagaaac atgtggcgac ttttgtctgt gagccgaagc gctgtcaatt
    12841 gccgacttgg cagcgcttcg ccgctctgcg aactcggcca ccaaaacaaa aatgaaacgg
    12901 cacgtccaag aggcgtgccg tttcatcttc cgtcagaaac cgcggcggtt tcgaagttgc
    12961 gattcgctcg cgtgcaccgc gctgggttcg actcgcgtcc acgagcagct gagtcgcgtg
    13021 tgcgcgtgac gcggactcag ccgttgatct tgacgtcgga tacggagacg ttgacctccg
    13081 gcttcacgca gatcacctta cagatctctt cgacgccaaa cttcgtgtag tcgaacgtga
    13141 acgacgcatt cttgatgcga tagccgtcgc cttccttcgc aacggtgtag ttcacgttga
    13201 cgtccttcgc gcctttggcg agcgtgaggt gccccgtcac gcttccgctc gactcgccct
    13261 tctctggcaa cttcagcttc gacttatcga tagtgagcgt agctttgggg aactccttca
    13321 ccttgaagga gcgtttggtg tgatcgtcac gaacacccat atcgaggctg tccctgaggt
    13381 tggtgacgaa caccagcttc ccctcgttct cagacacgga gaactttgcg gtgcccgtga
    13441 aagaaaggcc gacggctttg ccgctgatct tgagcgggcc accgccagac atcgcggcgt
    13501 gggccgccaa ggtgaaggtc gtggcgctga ggaggagagc gaaagcggta cggcggaaca
    13561 tcaggggaac tcctttgcta cgtattcccg aagcgtctgc ttcgtatgtc cgaagtggat
    13621 ggggtcatcc atcgacgggt tgagcccagc tgttattcag cgtgcgtcgt cgacgcggtg
    13681 ggcttgaagg tgaatcgatt gaaacgaaga tggggagggg cttttggggc gactcgcgga
    13741 cttgctttgg ctacttgcga ctttggaaag cgtgggggtg gggaggcggg catcgagggc
    13801 cgcggctttg attgtcccga ttgggttctg cgcgatgcgg tatcgacttt ttaggtgccg
    13861 cggggcgcgg ttttggttcc agaaattttg atttctggaa tctttcccag cggccaatcc
    13921 accatgggtt gcaggagagc ttacctcgaa gcggctacaa atcttccttc gtaagtgcga
    13981 gcgagttgca ggtgtcgcag acccggtggc gcgcgtactc aacgcggttc gctctccaca
    14041 cgttcgaagc ggagtgcggt gcgctcgact ccggtgaggg gccaccgata ctcgagcctc
    14101 tgggcttctt gccagccctg caatgccggc gcatctgcct gggctagcag aacccaaacg
    14161 gaatgccggg ccaatcgcgc accttcttcc aaccattgtg gaggcggcaa agtggcacgg
    14221 gaaattgcca ctgaacatgc accgttcggc tgctcctcac tgcgtccacg gagaacgcgc
    14281 acggggatcc caagctcgcc aaccaccgtg cgcaggaagg cgatgcgttt ggccttcggc
    14341 tccaacaact gaattctcaa cgcgggaagc agtacggcaa ggccaagtcc aggcgcaccc
    14401 gcacccgtcc cgacgtccaa ccaagactct gaatcctgac tgcaattatt tgccactatt
    14461 gcggcatccg cgatgaggag atcgactaat tcctcgggac cccgcgcggc cgtcaggtca
    14521 atccgctgat tccactcgaa cactcgggtt gcccaagtgg acgccagtcc taagcgcgta
    14581 gagcgcccac tgggcagatc gggatccagc tccaaactga cgagggcccg tttaatgagg
    14641 tggttgaaat caggcattgg ggccaaagcg cgcccaattt ggcgcgctct tccccgcacg
    14701 tccacttctc tcggactcgt gaggtatcca cgccgtatac gcagaatggc cgggctgctc
    14761 gcagtcgagt aaaaggggcc gaatgcccgc cgcggatttc ccactccata cgctcgagcc
    14821 cctggttcga ttggccaccg gcgtcgagcc gcccctcgag ttttcggaga tcttcggtgg
    14881 agcctcggtt cggcgtttct tccgggtcaa ggccggcccg ggctcgagcc tcatcgcgat
    14941 gtatgtgcca gcccaaagtc acgagctgag taagccgccg agtaagccgc gccgctggcc
    15001 ttttctggaa gtccgcgagc tgctcgagga gcgtggtgtg cgcgtcccga aattgttggc
    15061 cgaagactgc gccaacggct ggattctggt cgaggatttg ggcgacacgc tcgcacaaca
    15121 cttgaacaag cacccggaag agcgtgaccg gctgtatagc gccgccgtac gctcgctatc
    15181 ccttgcgcaa cgcgcgttac gcgagttgcc ggaacactcg gtgatcctcg agcgagcgtt
    15241 cgacgaagat ttgctgacct gggaactgca tcatttccgt gagttcggtc ttgaggcgcg
    15301 aggcatcagc ttgaatccgc gacaagccga ggtgttcgag cgcgccgtag cccatatcgc
    15361 gagcacgatc gccgcctggc cgcgaggttt cgtgcaccgg gactatcaaa gccgaaacat
    15421 catggtggtc gaaccgcacc agccggagct cggatggata gactttcagg acgcgatgtt
    15481 ggggccgcgg gtttacgaca tggtggcgtt actgaatgac agctaccaga acttcgacgt
    15541 ggcgttcgtc tcgacgcgtt tggacgagtt cggggccgca ctcgggttgg ccgccctgga
    15601 gcgcgaagcg ctgaagcgtg agtttgcctg ggtgaccgtg cagcggaaac taaaggacgc
    15661 cggccgcttc atcttcatcg aacgacgcca cgacaatcct tctttcctgc ccttcttcga
    15721 gcccaccctg aggaaggcgt acgccgcact gcgttcactg aacgacggat gcctgggcga
    15781 tctaggcgat ctcttggtgg agttatttcc gatgctgaac gatctagacg ttcgacgcta
    15841 agaacctatc ccagtaggag cgccggcgac tagctcctat ggggatgggt tctaagcgcg
    15901 gtgcacgggc ggagcctccg ccccgggcca gactcccaac cattcctcgg cgaattctgc
    15961 gtcgaacggc aggagccaag cctgctcggg caactcacgg cgttgcgccg cctccgccat
    16021 caaggccaga ccgcgctcga gcgagctcag tgcgcaatgc acgtacgaat aggccagacc
    16081 gaggcccgcc ccgactacgc gacccgcctt tgcgacgagc agcgaccgat ccagcaggtc
    16141 ttctagttgt tgacgctctt cgagccgcac ttccggagcg ccccgacagc gatacttgat
    16201 gtagaagaag cgctcgccga agcgcgagaa gcgctcggac gcgaaggaca gcccggacag
    16261 gtagcacttg agtgcctcgg gcacgcaggt cgaagcgaac accaagtcgt cctgtgccgc
    16321 gtagtctcgc gcgggctccg gttcgagctc gaatagactc cactcttcct ggtgactcga
    16381 ttcgccgtat ggagagtccg gcaaccccgc gtacagcccc tgaatggcgg ctttgaacgt
    16441 gggcgccagc tccgcgacca ccaagtttcc gctcggcgcg cccgagtcca atactttgag
    16501 tggcccgagg cgcggcgccg gaacgaccga cacgcggccg agccaggcga gggccaggcg
    16561 gggaccgagt aggtcgaaca ccaagcgagt ggcggcctcg atttcgcgtt cggagcccga
    16621 gcctccgggc aatgcaaaca ccacgtccaa caagtgcccg cgcgcgaagc cgacacgcag
    16681 acgcgcctgg gagaagtccg cgatttcccg agcacgtttc aacgcttctt ccagcggcag
    16741 caccggcgcg agcgcgatcg ggggcgcctt cgagccgagc tgggtaacca ggtgatctac
    16801 gagaggcagc agtgcagctt cccggggccg caatgccaac gcgccgttgc ccgagtgttc
    16861 gagccacagc tcggggcaaa ttgcgccgag cacaccctcg cccagcattc gcgccgcccg
    16921 ctcctcgatt tcgtgtagcc ggcgctgctc cgcctcgttc cgctgcccaa aacgaatcct
    16981 cacttcaatt gcgctccgcg atcgagctcg gacgacgtcg aggccgcgcc ttgttcttgc
    17041 gcttcttgcc tccgggcttg ctcgcgtcgc gccgctcccc gtacaggacc acgtctgcgc
    17101 gcaccggcat cactccgcgc cgcaacagcc cgagctgtcg cgcggcggct ccagaaaggt
    17161 ccaagatccg gcctcgcacg aacggccctc gatcattgat ccgaacgtac acctcttcgc
    17221 ccgtgtcctc acgtaccacg cgcaccacac tcccgaaggg caatgagcgg tgcgctgcgg
    17281 taaagcgcgc gggctggtag ggctcaccgc tcgcggtagg gcggccactc agcgagtcac
    17341 tgtaatagct cacacgtccg cgaaaatgct gcaacgcccg cgcgttgcgg aattgctcac
    17401 tgagcatgcg cccgtcgttg gcctgaggct cggcccaaga cggtgccact agcggtcgcg
    17461 cgggcaccac ccgctccggc ggctcgaccg gtcgcgcagg tgccatcggc cgttcgcagc
    17521 cccagagggt gaccaggacc gcgaggacgc tgagccgaag caatcggggc gcgaccactg
    17581 accttccggc tatcacgcga tctcttgacg agccaggatt ggaaacgatg ccggcccgcg
    17641 ccttccccaa aggaaaatag gtttaccgaa ccggtgaact caccaggatt tttgactttt
    17701 ccaagaagtt gcggagacgc gaaacgaaaa gagtcgagct ctcgtttggt ctcggggccg
    17761 tgatataggt gccgcctcat gacgccccga gtcgctcgat ccaaacccga tcgtgttcgc
    17821 ctgacgcgga gcgggctttc tctcggcgac accaatctac ctctgttggt gggcagcgtt
    17881 cactactggc gtcttgatcc agacgtgtgg cgcgacgcgc tggaagcggt caaggcgctg
    17941 aacctcaagg tcgtcgacac ctacgtgcct tggagcgttc acgagcgctc gcccgggcac
    18001 tacgagttcg gggagaacga gcgccatctc gatttggtgc acttcctaca gctcgcgcac
    18061 gagctcggct tgtacgcctg cgttcgcccg gggccgcacg tgaacgcgga gctgactcag
    18121 ttcggcattc cagaacggat catctgggat ccgcaatgcc aagccctttc ggcgcgcggc
    18181 gcgcgagtgg tgctgccggt cccaccgctg gcctttccgg taccgagcta cgccagccgt
    18241 accttcttgc gcgaagcttc agtttggctc agcgcggtcg gcgatcggat agctccgctg
    18301 tgttatccgc gcggcccggt ggtactttgc caagtggaca acgaaggcgc catgtacttt
    18361 cgtgacggcg tctacgatca ggactaccac ccggatgcga tccgcggata tcacaagttc
    18421 ctgaagcgaa agtacggcac acccgcggcc ttgtcgcagg cgctgggacg caacgtcgag
    18481 agcttcgaga tcgagccgcc gcgtgggctc gaggccaaca cctcccgtga gctgaccccc
    18541 cacctagact gggcggagtc tcaggaagag ctgctctccc actcgttcgg caagatgcgc
    18601 gaggcgctgg aagaggcggg cgtagacgtg cccttctcgc acaacttccc gatcggggaa
    18661 acgctcactc ccctcgatcc caagcgcgtg cagaaggtcg tggatttggt cggcctcgac
    18721 tactaccacg cggcgtctcc cacccagcgt caggaaatcg ctcgccgaac gagtgacttg
    18781 gccgcgcgct ccgagctcga gcacgtgccg gctttcgcgt gtgaaattgg cgctggattt
    18841 cccccgttct ttccgccttt gaccgagacg gacaacacct tcacgctgct gaccgcgttg
    18901 gcttacggcc tgaag
//
LOCUS       DASTKU010000923        21727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_402654, whole genome shotgun sequence.
ACCESSION   DASTKU010000923 DASTKU010000000
VERSION     DASTKU010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 21727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21727
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402654"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="VFQ61_32540"
     CDS             <1..1451
                     /locus_tag="VFQ61_32540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959279.1"
                     /translation="RLRQEEVERNTSQTGTPRLDANRSEIVARVKSEPVLYWEPPRIR
                     EGELPLPLAQLREAFFDGARPGRALEAIYRRLRRSPSDMRKVLLTEGYVYAESPGLAV
                     LLGRVALDHLFEDAEVFVLRGARLLRAKREKNVYVWQDGPERGTPARLWLFDRVGSSV
                     DQLLPAKHVAVPASAVLPARAFAVERLTERGVLAQATIGDRTARVLFSVQDGGLRFDC
                     ARGLDRTTLDVERARMLRERRVLAAMEAGIAEQLAEGLPFDEPKTEDGQQDGKLRPEW
                     RKAYREGLDSFEFNGDTYDVFDSSGRPRVPQVCADFVVDTWERMAGTRWLPRQQGRGR
                     RVGRLDFDALPIENRRSVEQLIEFARMHPEWFELLEIPEHRRVAFSERKRFFRRLYEQ
                     RDAFRPGDVVAVLGPRDDERLHYHSFFIVAADPITGMPTRVAANAGRPRIRSWEAELE
                     NAPKRGIVARIRPHLEWLEALLQPETASTQGT"
     gene            1561..2877
                     /locus_tag="VFQ61_32545"
     CDS             1561..2877
                     /locus_tag="VFQ61_32545"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET9959280.1"
                     /translation="MRQERKKSPLRIGLLGCGVVGGGVIRLLNENKSYLELRVGAPLE
                     ISHVLVRDAHKERVPECKAEWLTTNPESVLADDSVDLVVEVMGGEEPAKAYIERAIAA
                     GKGVVTANKLLIAKHGPVLIRSAIERGVDLAFEASVGGGIPIIRTLREALASDSVASV
                     HAILNGTCNYILTRMREAKLAFSEALGEAQKLGYAEANPSLDIDGHDAAQKLIVASML
                     AFDAQVDPSSVLVEGVRAIDQVDFQFADRFGFTLKHLGIGHDHGDRLELRVHPALIRK
                     NSVLANVDGVLNGVFLMGRALGPCLLVGRGAGDMPTAVSVVADIVDVARSRLEGEPGL
                     STRSIQLSERKLVPLDEIQTRYYLRFDVGDHPGVVGSIASALGQEGVSIEQMVQEGRA
                     QAADDGVPVCLITHVTREGNVRRALTAIAKQSFLKTPPRLIRIEDI"
     gene            2874..3710
                     /gene="murI"
                     /locus_tag="VFQ61_32550"
     CDS             2874..3710
                     /gene="murI"
                     /locus_tag="VFQ61_32550"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET9959281.1"
                     /translation="MRDLPLGVFDSGLGGLTVVRALRDNFPNERITYLGDNARVPYGT
                     RSAETVTRYAEGCARVLSRRGVKALIVACNTVSAVALEFLRERLDMPVLGVVEPGARA
                     ALSALHAGQTAAPRIGVLGTAGTVSSGAYPRAVGQLSPRHEVIAYAAPLLVPLVEEGW
                     IDGEVPRLVVRKYVAPLVEAGVSVIVLGCTHYPLLAPLIAEEAARLAGHPIPVVDSAA
                     ATAEAVRTWFSEERVPRTAQNTGGELELLVTDRPQSFGVVAQRFLREPLPPVELIDLP
                     TS"
     gene            3824..4717
                     /locus_tag="VFQ61_32555"
     CDS             3824..4717
                     /locus_tag="VFQ61_32555"
                     /inference="COORDINATES: protein motif:HMM:NF004957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET9959282.1"
                     /translation="MAAVLPIDLRGRRALVVGVSDDTGFGFAIAKTLAEAGASVCVGT
                     WPPALGIFETLLRRGKLDDALGLSDGGKLDFERIYPIDAAYDTIADVPESVRESKRYA
                     GRGDFSISGMVQSLVNDFGERPIDILVHSLANGPEVHLPLIETSRAGYLAAVGVSAYS
                     MVSMVSRLAPIMRPGGSCISLTYLASERVIPDYGGGMSSAKAALESDTRVLAYEAGRR
                     YGVRVNTISAGPYASRAANVTGIIQNVIAYYEKNAPLTEKLTPREVANAVAFLVSPLA
                     TGITGTTLYVDKGYHAMGKGE"
     gene            complement(4693..5082)
                     /locus_tag="VFQ61_32560"
     CDS             complement(4693..5082)
                     /locus_tag="VFQ61_32560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959283.1"
                     /translation="MKHANHAGYFRGEGRARRIAIDGLPLILSLLTDSLATKTKALPY
                     DEQTPSFRALLPPRCSPLAPCLMGRHLVRVPHRYTRTSATCGSFSANFLRRSHYLVDT
                     VDLSAPATLAGTDCPKLGPGYSPLPIA"
     gene            5157..6554
                     /locus_tag="VFQ61_32565"
     CDS             5157..6554
                     /locus_tag="VFQ61_32565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959284.1"
                     /translation="MNPVPALPEPLADDPEVVVTALETAEIFKAQGNLREAVRWVRRA
                     AELAGNAGLDERALMLARSAADAASALVESVPPPAAQPPAAQSSYAPAPAAQPSYAPA
                     PVVAPPAAAAAHAATEMMAAVAPGVDLSSGPIATGSTPAAPAPAVQVASAPSVPPPAV
                     AAAAAAVAPVAVAPPPPPPPPPPPPPPPPPIVPARATAHGGSTPPPSLESRADALRTE
                     PPALQRGPEPSATPSVSVAPIVSVASSSGSSFSGATSQNASSLNTDEHAPPPATVRQD
                     PIAALAQAQASVGKTQTLGEAAVPFAATVEFPMRAPAATVVMAGPAAAAALQQASAPN
                     SLSGNGISAGGNQQPIASAPSEAARNGSQASAAARSPSGGLPGTDAVRAKGPTWGEQP
                     PGVASGKPSSTAQDSGAARAAARPAVRASVERGDDEGVFIVRLLSPTEQPKPGAQEAF
                     VVLTDPSSTLFKSRA"
     gene            6679..8550
                     /locus_tag="VFQ61_32570"
     CDS             6679..8550
                     /locus_tag="VFQ61_32570"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9959285.1"
                     /translation="MSGIEMEQAVAARRLGELIGRYRLERVLGAGGMAVVYAGRTPEG
                     AVAAIKVLFTDMNVRRDVRERFYREGYLANSIQHPAVVRVLEHGETQDGVAYLAMELL
                     EGETFAARAQRLGALGVAELLEVADQVLDALIIAHQQGIVHRDLKPDNLFLTRDGQVK
                     VLDFGLARLLDALPQDRRTRSGVALGTLPYMPPEQALGRRAEVDARVDLFALGATLFR
                     LHSGRRIHEAESDAELLMCMASKPAPPLSSVLPSAALGFCQIVDLALAFARDARYPDA
                     RTMQSDVRAVRAGQSPPFASQRLELRDQKTHQVPMAAEAPLGATFTSAAPLGLSALNG
                     PGGRAGGGAPGSEPRLQVGGTVPIGLEAPQPFAAMPDPAQFGQTFRSTYAPPGASPVS
                     PQRAPVIVEFVRPAQPAPNEGFGQTIPSATEGVVPHSPEPPQPRINPPSPAPPRDILG
                     AETQLASTGWNAGVSAPSTSRTPPPPIWALLLLAAMAMGAFAALMVLVWWFLVAAGGP
                     TASPPAPTAVPSVPTAHAPGANSGLGSSGSLQGGNVVNGPIVAPVNEGNSPGSPSATG
                     PANPTGHSDVKAPRVPSSTPAPNVNQPAPSASPTDGRTDPDSRVLEDRGPFRNRQRD"
     gene            complement(8589..9533)
                     /locus_tag="VFQ61_32575"
     CDS             complement(8589..9533)
                     /locus_tag="VFQ61_32575"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET9959286.1"
                     /translation="MTEPRLRHLLGIEDLSVAAAQRILNTAEAFYEVSRRPVRKVPTL
                     RGKTVLNVFYEASTRTRTSFELAGKRLSADVVNISGSSSSVSKGEDLRDTVATLDAMH
                     ADVVVLRHHASGAAHYVARHTRAAVVNAGDGMHQHPTQALLDAFTIRRQLGRDIAQLR
                     VAICGDILHSRVARSNALLLQMLGAEVRLSGPRTLLPTQPELFGARIYDRVEPALEGA
                     DVVMVLRIQRERLGAALLPSLREYSRYFGLNAARLALAQPHAIVMHPGPMNRGVEISE
                     DLADGPRSVILNQVEAGVAVRMAVLYHCASEPGVAPTE"
     gene            complement(9530..10090)
                     /gene="pyrR"
                     /locus_tag="VFQ61_32580"
     CDS             complement(9530..10090)
                     /gene="pyrR"
                     /locus_tag="VFQ61_32580"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET9959287.1"
                     /translation="MQVLLDAEGVQRALRRLAAELVQRAPQLDDLILIGIRRGGVPLA
                     EQIAQYLNEMTGRQVSMGSVDITFYRDDVATRLPDPRIGPSQIPGQLDARHVVLVDDV
                     LFTGRTVRAAIDALMDYGRPPRVELAVLVDRGGRELPIQADYVGIRTEVPPGQRVDVV
                     PVMGHPCAVLRPEGSPSLPPPQEIDR"
     gene            complement(10179..10727)
                     /gene="ssuE"
                     /locus_tag="VFQ61_32585"
     CDS             complement(10179..10727)
                     /gene="ssuE"
                     /locus_tag="VFQ61_32585"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0044273 - sulfur compound catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HET9959288.1"
                     /translation="MTTTLIIAGSPSGTSRTLRVAEAVGKRLTSLGTTVSVLEVRKLP
                     SEALLHAKFDDPALVEAIQQVEAADGIVIATPVYKAAYSGLLKAFLDVLPQFGLRGKV
                     VLPLATGGSLAHVLAIDYALRPVLSSLDAWHVVSGLFLLDRQLVPTEAGGIEIEPEVS
                     ARLDVLLNAYVQSLNAARALQF"
     gene            10976..12271
                     /gene="murA"
                     /locus_tag="VFQ61_32590"
     CDS             10976..12271
                     /gene="murA"
                     /locus_tag="VFQ61_32590"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET9959289.1"
                     /translation="MNSLLIEGGHPIAGEIQIAGNKNAALPMLAAALLTDQPVFFRRV
                     PEIRDVLTMQATLSELGVDVSREDGGRVTCRAGRLTTTTPSPALCQKTRASFLLAGPL
                     LAREGRAELPSPGGDKIGRRPVDTHIEALRELGAEVEIRPDRYVMRAPNGLVGRDLFL
                     GEMSVMATENVLMAATLAKGTTVIRNAASEPHVQDLCRMLVSMGARIEGIGSNTLTIE
                     GQPKLSGCTAEVGPDYLEVGSFIALAAMTGGQLWIRGACPSQHRVTANAFGKLGVRFE
                     SHGDDIFVPRHSELRVEEGLHGALPKIHDAPWPGFPADLTSIALVLATQAQGTTLIHE
                     WMYESRLYWVDRLIAMGARVTLCDPHRAIVIGPAKLYAQNVSSPDIRAGMALVIAALV
                     AEGKSTIHNAQQIDRGYERIEDRLRAIGARIERVEARKS"
     gene            complement(12413..13228)
                     /locus_tag="VFQ61_32595"
     CDS             complement(12413..13228)
                     /locus_tag="VFQ61_32595"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET9959290.1"
                     /translation="MSYPETPLQAAFPRPGRVLKVVMLGIFGVWLAFAIAINWADAPA
                     SLFQALCGNTERILHGEVWRIFTAPWLHMPTQTLGHVLSALMGLYFLAPSLEERWGSA
                     RFARFLFVSALIAYGTQMVAELVLPHSIRARLVDDYWFGAIPVVEAIAIAWALSFRGQ
                     VVRLFFVLPVSSTGLIVFVIAASLMYIVIGERGPAGAIAPFGGMLAGWLFGGSTPSPF
                     RRLWLKLRLAQLDAEARREARDRRKRVERSDLRVISGGKKTDRENGSGDRFLN"
     gene            complement(13248..14378)
                     /locus_tag="VFQ61_32600"
     CDS             complement(13248..14378)
                     /locus_tag="VFQ61_32600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959291.1"
                     /translation="MKSRSVRLCSAQSWPTQSGRARFWTATAACAPLFGALACGSSSG
                     ATTTEPHPTIVEVGPSAFDGALACSAAGVGTLRSYVATLKDETASRPGSSFYLPSSSA
                     TPCQRAVAFGAISPLHRYSVELDGYDRDGLEPFAPGLRAQRDPETNELVTPRWKATCE
                     AVQAEYQLTVPVLLCSAWSNGGSTDPGAEARVSLEVAQVLGAECEASTPRVDRVEVRS
                     EGEVLAEGACGETLTLTFSDPSDSVHELELLAYAPSAEQPLLGGVCEVRTAPGTTLPA
                     TCAPLSDVGGIHVQVSALLQALQVRCEDLGRLVVSLDATESKLTFDSHSCNGNAAFMA
                     VEPGTHVLDVEAWARDSTQIGQTQCTADVEPGLVSSMACGSR"
     gene            complement(14439..15164)
                     /locus_tag="VFQ61_32605"
     CDS             complement(14439..15164)
                     /locus_tag="VFQ61_32605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959292.1"
                     /translation="MKSLRAASLLLFSAVVGALLSGPGCGTDARGVDACRRIEQARCE
                     AAPACGLISDASACSRFYRDQCLHGLVARDPAATQVGECEAAIRAAGACAAANQDTAS
                     ACTGRQTPASACEAIEHPEKLRACAFLDPSPSETGEGGNGGAEGTAAGNGGVESAGTG
                     NGGGATNMSTSGGETGSEDPTGGQLNGSGGEVNGSGGTPETSGGGAGTTSNAAGSSGD
                     GGSTSSGNPSSEGGSAGAGTAGT"
     gene            15282..16196
                     /locus_tag="VFQ61_32610"
     CDS             15282..16196
                     /locus_tag="VFQ61_32610"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9959293.1"
                     /translation="MPRALVLSAGFGSRLRPLTEELPKPLLPFGDRCLLDHALRALDG
                     RRLSGDVMVNVHHLAGCFEAYRERLSRPVELIHESNIRGTAGGIAGTRERLDGSGLVV
                     AIADVVLDQVPEAFMEAPAWGSLVLALRRAPAGRGNVGVGQGGEVVRLRGERFGAEAW
                     SGNYVGLCALSQAVVADLPDVGCLIGDYALPVLRSGGRVLAFEFDGECSFPGDDLPGY
                     LAANLAWLQRRNLEQFCAPGSQIAAGVELRRALLSRGARVSGSGRLERVIACEEAEIQ
                     APLQDACVLPSGRVVPIAPPTLAQSEGA"
     gene            16431..17255
                     /locus_tag="VFQ61_32615"
     CDS             16431..17255
                     /locus_tag="VFQ61_32615"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HET9959294.1"
                     /translation="MNVRNLEQIQEQDSAPRRSRIGAILLFAGACAAVVTSSAMMIKR
                     KGPAAVSTADPLAALVSSARSATPADKLEGRDVTFPSVLSDSERTTTALAAVKDERGR
                     LVPRVEAPQPENPPAPAERLPVVPLPVGALLGETPVTAAPKDRLTALASDALRSGESQ
                     SLAPAGMDGGFQLQVASFKEQAEADKLVEELRRRGHRAFRQAAYVSERGLWHRVRVGP
                     FKSRLEAQKYKAEFEKNERLSPFLVDPEKVKQAEAMRAARLAAQDKRGSRRPVPGE"
     gene            complement(17358..18482)
                     /locus_tag="VFQ61_32620"
     CDS             complement(17358..18482)
                     /locus_tag="VFQ61_32620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959295.1"
                     /translation="MLTSPDFSSLSPVAQKILNPDAPAPLRLMAARGALPGVKPHELV
                     CVVALLTDSPDEKAREAATATLGKLPQALLDGALNAELPALVVDKLARAYLNQHAVLE
                     RLLRQKSVDVSTLEQLASAADERSGEIIATNEELMLANPTVIEKLYMNKQVRMSTADR
                     LLELAVRNGVEVAISAYKEIAQAIKNELIAEASPEPTYDDIVFHDTEQTAERAGPLAT
                     DEDTHEVDDEGAEKLRERFVPLYAIIGTLSVTQKIRRAMLGTAAERLLLVRDSNRLVA
                     TAAVKSPLMRENEATQISASRSVSEDVLRAIAQNREFVRHYQVKLNLVANPRTPFTFA
                     ARLMPHLRDNDVRSLAKSKNVSGAIALAAKQQLLRKQGKS"
     gene            complement(18628..19974)
                     /locus_tag="VFQ61_32625"
     CDS             complement(18628..19974)
                     /locus_tag="VFQ61_32625"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9959296.1"
                     /translation="MTDFLERIESAREHVSPRWTAARERSLRWQLESRLERRRTTGRV
                     LAGMALVVALIVGHLGYSQWIDGAPKGAVGQRAQTTKGARTGIGGAAAYQGAEARAAS
                     GRGERVGGAEAETQRGSALKAAVGVASVQLTDGTVAQGMSADAHLLTLEESVQRVTMR
                     LASGRARFDVTPRAERTFRVEARNVAVTVLGTQFTVELLADGVRVGVERGRVEVEWPG
                     ERRVLGAGEEVTAGGEEATVGESSAVGGGAASAAGAANPGAQNTAAPGITPPGGGTAA
                     AGTSVNGNSMLGSAAASKNWRSLAQEGDYAGAFAVLSQEGAGSIRQEPGDLLFAADVA
                     RLSGHPAQALPQLERVVRSYPSDSRAPLAAFTLGRILLDSLGRPREAAEAFARCRKLA
                     PGGALSQDALAREVESWSRAGEATLARDRAIQYLRLFPGGRREKAVRYHGGLEGTQ"
     gene            complement(19971..20588)
                     /locus_tag="VFQ61_32630"
     CDS             complement(19971..20588)
                     /locus_tag="VFQ61_32630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9959297.1"
                     /translation="MQRAHAALQVVPSVAAPHERVEVPPGEVEIPSEVVMPGEVELEL
                     QLDFDTLYRRYSPYVAAVAHRLLGQDAEVDDTVQEVFIAAVRGISQLRDPLAVKAWLA
                     RVAVRAARRKLRMRRMRSFLGLDTPAAYQAVADDRASPEQRALLARVYKVLDGIPTNQ
                     RIAWTLRFIEGEQLEAVAELCGCSLATAKRRIQAVSRLLEEAFAE"
     gene            complement(20951..>21727)
                     /locus_tag="VFQ61_32635"
     CDS             complement(20951..>21727)
                     /locus_tag="VFQ61_32635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959298.1"
                     /translation="KTLLRTFDEGSLPPRARARLVAIAAAELVLASWAELTYQPSLRV
                     QPLGPVASEKDVHAVLARLEAGTGVRVADAETPLPAAPMSRPWYAPLPREERMFHLSA
                     LGSVRAFLQHPGQLWGAGVRVSEEALRATSWSVDALYEGGTLTGKQDLRYRIQTWSFA
                     ASLLLYYRTGIVTARAGAGLRVGAVKAESLDRDLGRAKNVLVPWGWPLLVLSLSARSG
                     PVVLNVSAEGSYAAIPVAGGTITPISGIWVGTQWGIGFIP"
BASE COUNT         3683 a         7069 c         7131 g         3844 t
ORIGIN      
        1 gcaggctgcg gcaggaggaa gtcgagcgga acacgagcca gaccggcact ccacgtctag
       61 atgcaaatcg ctcggaaatc gtggcgcgcg tcaaatccga gccggtgctg tattgggagc
      121 cgccgagaat ccgtgagggc gagctacctc ttccgctggc tcagcttcgc gaggctttct
      181 tcgacggtgc gcggccgggc cgggcgctcg aggccatcta tcggaggttg cggcgctctc
      241 cgagtgacat gcggaaggtg ctgctcaccg agggttatgt ctacgccgag agcccggggt
      301 tggcggtgct gctcgggcgc gtagcgctcg accacttgtt cgaagatgcc gaggtgttcg
      361 tactgcgcgg cgcgcggctc ctgcgagcca agcgggagaa gaacgtttac gtttggcagg
      421 atggtccaga gcgcgggacg cccgcgcgac tctggctgtt cgatcgggtc ggatcgagtg
      481 tcgaccagct cttgcccgcc aagcatgtcg cggtgcctgc ctccgcggtc ctgcccgctc
      541 gcgctttcgc ggtcgagcgc ttgaccgagc gcggcgtgct cgcgcaagcg acgatcggag
      601 accgcacggc gcgcgtgctg ttctccgtac aggacggcgg cctacgcttc gattgcgcgc
      661 gcgggctcga tcgcacgacg ctcgatgtcg aacgagcccg gatgctacgg gagcggcgcg
      721 tgctcgccgc gatggaagcg ggcatcgccg agcagttggc ggagggcttg ccgttcgacg
      781 aaccgaagac cgaagacggc caacaagacg gtaaattgcg acccgagtgg cgcaaggcct
      841 accgcgaagg cttggacagt ttcgagttca acggggacac ctacgacgtg ttcgactctt
      901 cggggcgccc gcgtgtcccg caggtgtgcg ccgatttcgt ggtggacacg tgggaacgca
      961 tggcgggcac gcgttggtta ccgcgccagc agggccgagg gcgccgcgtc ggtcgcttgg
     1021 atttcgacgc gctgccgatc gaaaaccggc gcagcgtcga gcagctgatt gaatttgcgc
     1081 ggatgcaccc ggagtggttc gagctgctcg agattccgga gcatcggcgg gtcgcatttt
     1141 ccgaacgaaa gcgttttttc cggcgattgt acgagcagcg tgatgctttc cgccccgggg
     1201 acgtggtggc cgtgctgggc ccgcgagacg acgagcgcct tcattaccac tcgtttttca
     1261 tcgtggcggc tgacccgatc accggcatgc ccacgcgcgt cgccgcaaac gccggtcgcc
     1321 cgcggattcg aagttgggaa gccgagctcg agaacgcacc caaacgcggc atcgtcgcga
     1381 ggattcggcc gcatctcgag tggttggagg ctttgcttca gccagagacg gcatccacgc
     1441 aggggacctg agagaagcgc tgctcgagag gcgacccgaa cggatgcgaa atcgtgggct
     1501 cggccgcttc ctgcttgacc tccagggaag gcccgcggca aacaccggaa cggaggcccc
     1561 ttgagacaag aacgaaagaa atcccccctg cgcatcggct tgctgggttg cggcgtcgtg
     1621 ggcggcggcg tgatccggct gctgaacgag aacaagagct acttggagct ccgcgtcggg
     1681 gcgcctctcg agattagcca cgtactcgtg cgagacgcgc acaaggaacg cgtgccggag
     1741 tgcaaagccg agtggctcac cacgaacccc gagagcgttc tcgccgacga ctcggttgat
     1801 ttggtggtcg aggtcatggg cggcgaggag ccggcaaagg cctacatcga gcgggcgatc
     1861 gcggcgggca agggggtggt taccgcgaac aagcttctga tcgccaagca cggcccagtg
     1921 ctgattcgaa gcgctatcga acgcggcgtc gacctggcgt tcgaagcgtc ggtcggcggc
     1981 gggatcccca tcatccgcac cctgcgcgag gcgctcgcta gcgactcggt ggccagcgtg
     2041 cacgccattt tgaacgggac gtgcaactat attttgacgc gcatgcgtga ggccaagctc
     2101 gccttctcgg aggcgctcgg cgaagcgcag aagctcggct atgccgaagc caacccgagc
     2161 ttggatatcg acggccacga cgcggcgcag aagctcatcg tcgcgagcat gttggcgttc
     2221 gacgctcagg tggatccgag cagcgtgctg gtggaaggcg ttcgagccat tgatcaggtc
     2281 gatttccagt tcgccgatcg attcgggttc acgctgaaac acctcggtat cggtcacgac
     2341 cacggggatc gtctggagct gcgcgtacac ccggcgttga ttcggaagaa cagcgtgctc
     2401 gccaacgtgg atggtgtgct gaacggcgtc ttcctgatgg gacgagctct gggcccgtgt
     2461 ttgctagtcg gccgcggggc aggggatatg cccaccgcag tgagcgtggt ggccgatatc
     2521 gtggacgtag cgcgctcgcg gctcgaggga gagccggggc tctcgacgcg cagcattcag
     2581 ctcagcgaac gcaagctggt gcccctggac gagattcaaa ctcgatacta tctgcgcttc
     2641 gacgtgggtg atcaccctgg cgtggtgggc agcattgcct cggcgctcgg gcaagagggc
     2701 gtcagtatcg agcaaatggt gcaagaaggg cgcgcgcagg ccgcagatga cggggttccc
     2761 gtgtgcctca tcacgcacgt gacgcgtgag ggcaacgtgc gccgtgcttt gacggcgatc
     2821 gccaagcagt ccttcctgaa gacgccgccg cgactcatta gaatcgagga catttgaggg
     2881 atcttccgct cggcgtattc gactccgggt tgggcggcct gaccgtggtg cgcgcgcttc
     2941 gggataactt cccgaacgag cgcatcacgt acctcgggga caacgcgcgc gtaccctatg
     3001 ggacgcgctc cgccgagacg gtgactcgtt acgccgaagg ctgcgcccgc gtgttgagcc
     3061 gccggggagt gaaagcgcta atcgtggcgt gcaacaccgt gagcgcagtg gccctcgagt
     3121 tcctgcggga gcggctggat atgccggtgc tcggagtggt cgagccgggc gcacgggcgg
     3181 cgttgagcgc gctccacgcg ggccagaccg ccgcgccgcg catcggcgta ctcggtacgg
     3241 cgggcaccgt ctcgtccggc gcttaccctc gtgcggtggg acagctgagc ccgcgtcacg
     3301 aggtgatcgc ctacgccgcg ccgctactcg taccgttggt cgaagagggc tggatcgacg
     3361 gggaggtgcc gcgcctggtg gttcgcaagt atgtggcacc attggtcgaa gcgggagtgt
     3421 cggtaattgt gttggggtgc acccattacc cgctcctcgc cccgttgatt gctgaagagg
     3481 cagcgcgcct ggccgggcat cctattcccg tagtcgacag cgcggcggcg accgcggaag
     3541 cagtgcggac ctggttttcc gaggagcggg tgccgcgcac ggcgcaaaac acgggtggag
     3601 agctcgagct tctcgtgacc gaccggccgc agagctttgg cgtcgttgcg cagcgcttcc
     3661 tacgcgaacc tttgcccccc gtggaattga tcgacttgcc gacgtcttga gcatgtttcg
     3721 tcaatttcgt ggcccgacct ggcgcgttga ccaggtgcgc aaagcgctcg gcgtcgtgct
     3781 ggtttagagc ggaagtgttg cctcgagggg atttttgggc gatatggccg ccgtgcttcc
     3841 catcgatctt agagggcgcc gtgctctggt cgtcggggta tccgacgaca ccggctttgg
     3901 tttcgcaatt gccaagacgc tcgccgaggc gggcgcgagc gtgtgcgtgg gcacttggcc
     3961 gcccgcgcta gggattttcg agacgctatt gaggcgcggc aagctcgatg acgcgcttgg
     4021 gctcagcgac gggggcaagc tcgacttcga gcgcatctat cccatcgatg ccgcgtacga
     4081 cacgatcgca gacgttcccg agtccgtgcg cgagagcaag cgttacgcgg ggcgcgggga
     4141 tttcagcatc tccgggatgg tgcagagtct agtaaacgac ttcggggagc ggccgatcga
     4201 catcttggtg cattcactcg cgaatgggcc cgaggtgcat ctgccactca tcgagacgag
     4261 ccgtgctggg tatctcgctg cggtgggggt gagcgcgtac tcgatggtct cgatggtgag
     4321 tcgcttggcg cccatcatgc gtccaggcgg cagctgcatt tcgctgactt atttggcgag
     4381 cgagcgcgtc atcccggact acggcggcgg catgagcagt gcgaaggccg cgctcgagag
     4441 cgacactcgc gtgctcgcgt acgaagcggg ccgtcgttac ggcgtccgtg tcaacacgat
     4501 ctccgcgggg ccctacgcct cgcgagcggc caacgtcacg ggcatcattc aaaacgtgat
     4561 tgcttactac gagaagaacg cgccgctcac cgagaagctg actccgcgtg aagtcgccaa
     4621 cgcggttgca ttcctcgtca gcccactcgc caccggcatt acgggcacga cgttgtacgt
     4681 cgacaaaggc tatcacgcga tgggtaaggg cgagtaacca ggcccgagtt ttgggcagtc
     4741 ggtgccggcc agggtcgcgg gggccgacag gtcaacggtg tcgacgagat agtgtgacct
     4801 gcgcaggaag tttgcgctga aactgccgca ggtcgcgctc gttcgggtgt atcgatgggg
     4861 aacgcggacc agatgtcgac ccatgaggca aggcgcgagc ggggagcagc gcgggggcag
     4921 caacgcgcgg aagctgggcg tctgctcgtc gtacgggagg gccttggttt tagtagctag
     4981 ggaatcggtt agtagcgaga gaatcagagg tagcccatcg atggcaattc gacgggctcg
     5041 cccttcgccg cggaaataac ccgcgtggtt cgcgtgcttc acggttcact tctaggcttc
     5101 ggttccggta atctgcgtga ccgaccgttc gcgctgagcc gcgcgaggag aaatcgatga
     5161 acccagtgcc cgcacttccc gagccgcttg ccgatgaccc cgaggtggtg gtcaccgcgc
     5221 ttgaaaccgc cgagatcttc aaggcgcagg gcaacctgcg agaggccgtg cgctgggttc
     5281 gccgcgcggc ggaactcgcg ggcaacgcgg gcctcgatga gcgcgccctc atgctcgctc
     5341 gctcggcagc ggatgccgcg agcgctttgg tagagtctgt tcctccgcca gcagctcagc
     5401 cgccagcagc tcagtccagc tacgcaccgg caccagcagc tcagcccagc tacgcaccgg
     5461 caccagtcgt ggcgcccccc gccgctgcag cagcgcatgc ggcgaccgag atgatggcgg
     5521 cggttgctcc gggcgtcgat ctttcgagcg gacccatcgc gaccgggtca actccagcag
     5581 cgcctgctcc tgcagttcag gtcgcgtccg cgccgagcgt tcctcctccc gcagttgccg
     5641 cggcagctgc agccgtggcg cccgtagccg ttgctccgcc tccgcctccg cctccgcctc
     5701 cgcctccacc cccgccgcct cccccgatcg tgccggcacg ggcgacggca cacgggggca
     5761 gtactcctcc gccgagcctt gagtcacggg cggacgccct gcgcaccgag cctccagcgc
     5821 tgcaacgtgg tccagagccg agcgccacgc cgtcggtaag tgttgcaccg atcgtttcgg
     5881 tcgcatcgag ttcaggatcg tcattttcag gcgcgacgtc gcagaacgcg tcctcgctga
     5941 atactgacga gcacgcgcct ccccccgcga ccgttcggca ggatcccatc gcggctctgg
     6001 cccaggctca agctagcgtt ggcaagacgc aaactttggg agaggcggcg gtgcctttcg
     6061 cggccaccgt cgagttcccg atgcgagccc cggccgcgac ggtggtcatg gctgggcctg
     6121 ctgcggcggc ggcgcttcag caggcgagcg cgcccaactc actttcaggg aacgggatct
     6181 cggctggggg taaccagcag ccgattgcga gcgcgccttc cgaggccgcg cgtaacggct
     6241 cgcaggcttc agccgccgcg cgttccccaa gtggaggctt acccggtacg gatgcggttc
     6301 gcgccaaggg tccaacctgg ggtgagcagc cgccaggtgt tgcctctggc aagccttcga
     6361 gcacggcgca ggattcaggt gccgctcgcg ccgcagcccg ccccgctgtg cgcgcaagtg
     6421 tggagcgagg agacgacgaa ggcgtcttca tcgttcggct gttgagcccg acggagcagc
     6481 ccaagcccgg cgcccaagag gccttcgtgg tgctcacgga tccgtcctcg acgctgttca
     6541 aatcgcgggc ctgagttcct ccgcctggag tcaacgcgct ccgcgtctga ttcgatcgca
     6601 gctcgaggtt gaatcgccgc tggttttgat tcgtctgctg ctctaggctg agctggtcgc
     6661 agttcagcct gagcagccat gagcggcatt gaaatggagc aagcggtcgc ggcgcgccgc
     6721 ctgggcgagc tcatcggccg ctatcggctg gagcgggtac tcggcgccgg cggcatggcc
     6781 gtggtgtatg ccggccgcac gccggaaggc gcggtggccg ccatcaaggt gctgttcacg
     6841 gacatgaacg tgcgccgaga cgtgcgtgag cgcttttatc gtgaaggcta cctcgccaac
     6901 agtatccagc acccggccgt ggttcgagtg ctcgagcacg gtgagaccca agacggtgtg
     6961 gcttacctag ccatggagct tttggaagga gagacgtttg cggctcgtgc ccaacgtctc
     7021 ggtgcgctcg gggttgcgga gctgctcgag gtggccgacc aagtgctcga cgctctgatc
     7081 atcgcccatc agcagggcat cgttcatcgc gatctgaagc cggacaacct gtttttgacc
     7141 cgagacggcc aagtcaaagt gctcgacttc gggctcgcgc gactgctcga tgcgctgcct
     7201 caggatcggc gcacgcgatc cggagtcgcc ctcggaacgt tgccgtacat gcccccggaa
     7261 caagcgctcg gtcgtcgcgc ggaggtggac gctcgtgtgg atttgttcgc cctcggggct
     7321 actttgttcc gcctgcatag cgggcgccgc atccacgagg cggagagcga tgccgaactg
     7381 ctgatgtgca tggcctcgaa gcccgccccg ccgctctcga gtgtgctgcc cagcgcggct
     7441 ttgggctttt gccagatcgt ggatttggct ttggcgttcg cccgcgatgc acgttatccg
     7501 gacgcgcgca cgatgcagtc cgacgtgcgc gcggtgcgtg ccgggcagtc gccgccattt
     7561 gcgagtcagc gcctggagct cagggatcaa aagacccatc aagtgccgat ggcggcagag
     7621 gcacccctgg gcgccacctt cacgagtgca gctccgctcg gcctatccgc gctcaatggt
     7681 ccagggggcc gcgccggagg gggagctccg gggtccgagc cgcgcctgca agtgggcggc
     7741 acggtaccga tcgggctcga agctccgcag ccattcgcgg cgatgccgga cccggcccaa
     7801 ttcgggcaga ccttccgttc cacctatgct ccgccgggag ctagccccgt tagtccccaa
     7861 cgcgcgcccg tgatcgtgga attcgtgcgc ccggcccagc ccgcgcccaa tgagggcttc
     7921 gggcagacga tcccctcggc caccgaaggt gtcgttccgc acagtccgga gccgccgcaa
     7981 ccaagaatca atccaccgag cccagcgcca ccccgcgaca ttctgggagc cgagacccaa
     8041 ctcgcctcca ccggttggaa tgcgggggtg tctgcgccgt ccacttcgcg taccccgcca
     8101 ccgccaattt gggcactgct gctgttggcc gccatggcga tgggggcatt tgcggctttg
     8161 atggtgctgg tgtggtggtt tctcgttgcc gcgggaggcc caactgcatc gccgcctgcg
     8221 cctaccgctg taccttcggt gcccacggcg cacgcgccgg gagccaattc aggcttgggt
     8281 tcgagtggct cgctccaggg gggcaatgtc gtcaatggcc cgatcgtcgc cccggtgaac
     8341 gaaggcaatt caccgggatc gccgagcgcg acgggccccg ccaacccaac cggtcatagc
     8401 gacgtcaaag ctccgcgcgt tccctcgtca acgcccgctc cgaacgtgaa tcaacccgcg
     8461 ccttccgcgt cgcccacgga tgggagaact gacccagact cgcgggtact cgaagatcga
     8521 ggcccgttca ggaaccgcca acgcgattga gcgggcttct acggacgtga cgtttagtct
     8581 ttagccactc actcggttgg cgcgacgccg ggctcactgg cgcaatggta gagcaccgcc
     8641 atgcggaccg cgaccccggc ctcgacctgg ttcaagatca cgctacgcgg gccatctgcc
     8701 aagtcctcgc tgatctcgac gccccgattc ataggccccg gatgcatgac gatggcgtga
     8761 ggttgcgcga gcgcgagccg cgccgcattc agtccgaagt agcgggagta ctcgcgcaac
     8821 gacggtagca acgccgcgcc caatcgctca cgttggatgc ggagcaccat cacgacgtcc
     8881 gcgccctcca gcgccggttc cacgcgatcg tagatgcgcg ccccgaacaa ctctggctgc
     8941 gtcggcaaca acgttctggg cccggacagg cgcacttcgg cgcccaacat ctgcaacaaa
     9001 agcgcgttgg agcgcgccac gcggctgtgc aggatatctc cgcaaatggc cacgcggagc
     9061 tgcgcgatgt cacggcccag ctgacgccgg atcgtgaacg catcgagtaa ggcttgcgtg
     9121 gggtgttggt gcatgccgtc cccggcgttg accaccgccg cgcgggtgtg ccgggccacg
     9181 tagtgcgctg cgccggaagc gtgatgccgc agcaccacca cgtccgcgtg catcgcgtcg
     9241 agcgtggcca ccgtatcccg caaatcctcg cccttgctca cggacgaaga cgagccgctg
     9301 atattgacca cgtccgcgct caggcgtttg ccggcgagct cgaacgaggt tcgggtgcgc
     9361 gtagacgctt cgtaaaagac gttgagaacg gtctttccac gcagcgtcgg cactttgcgc
     9421 acggggcggc gcgagacctc gtaaaatgcc tcggccgtat tcagaatgcg ctgcgccgct
     9481 gcgacagaca ggtcctcgat cccaagcaga tggcgaaggc ggggttcggt catcgatcga
     9541 tctcttgcgg aggaggtagg ctaggggagc cctccggcct cagtacggcg cagggatgac
     9601 ccattacggg cactacgtcc acccgttgcc cgggaggaac ctcggtgcgg atcccgacgt
     9661 aatcggcttg aatcggtagc tcccgccccc cgcggtccac caacaccgcg agctccacgc
     9721 gcggcggtct gccgtaatcc atcagggcgt cgatcgcggc acgcacggtg cgaccggtaa
     9781 ataagacgtc gtcgacgagc accacgtgac gcgcgtccaa ttggccggga atttggctgg
     9841 gaccgatgcg tggatcgggc aagcgcgtgg cgacgtcgtc tcgatagaag gtgatgtcga
     9901 ccgaacccat gctcacctgt cgaccggtca tctcgttcag atactgcgcg atctgctccg
     9961 cgagcggcac cccgcctcgc cggatgccga tcaaaatcag atcgtcgagc tgcggcgcgc
    10021 gctgaaccag ctcggccgcc aaacgacgaa gggcacgctg cacaccttct gcgtcgagca
    10081 aaacttgcac gggcgcaggc tataccatgc tcctcggctc aaaccagagc gttcttcgtg
    10141 gcccttacgg ccgctctgcc cggcggctct agaaccgcct agaactgcag cgctcgcgcg
    10201 gcgttgagcg actgcacata cgcattcagc agcacatcga gccgggccga gacctccggc
    10261 tcgatctcga ttccgcctgc ttcggtcgga acgagctgcc ggtccagcaa aaagagcccg
    10321 ctcacgacat gccatgcgtc gagggaactc agcacgggtc ggagcgcgta atcaatcgcc
    10381 agcacgtgcg ccaggctgcc gccggtggcc agcggcaaga ccaccttacc gcgcagccca
    10441 aactgcggga gcacatcgag aaacgccttc aggagccctg aataggcggc cttgtagacc
    10501 ggcgttgcga tcacgattcc gtccgcagct tcgacttgct ggatggcttc gaccaaggcc
    10561 ggatcgtcga acttggcgtg cagcaacgcc tcgctcggta gcttccgcac ttctagaacg
    10621 gagacggtag tgccaagtga ggtcaggcgc ttgcccaccg cttcagccac acgcagcgtg
    10681 cgagacgtgc cagaggggct tccggcgatg atgagcgtcg tggtcattgc gttgaatgtc
    10741 agggatcccg aggggtagga gccttgcgaa aggtccagat aggcgcgcag caccgtatca
    10801 cgttgagcct cgagtccgga attttccgac ctagtggccg cgctcgatgc atgcagccgg
    10861 gaacaactgg gcgatatgaa ggggtgggtg gctttgggag tcggaggcgc tgaagttcag
    10921 agaacgaggc gcaggaaatg atgggcgttt cgtcagtccg cgctaggctc ggcccgtgaa
    10981 cagtttgctc atcgaaggtg gtcacccgat cgccggcgaa atccagattg cgggtaacaa
    11041 gaacgcagcg cttcccatgt tggcggcggc gctccttacc gatcaacccg tcttcttccg
    11101 gcgcgttccc gagatccggg atgtgctcac gatgcaggcg acgctcagcg agctcggggt
    11161 cgacgtcagc cgcgaggacg gaggtcgtgt cacgtgccgt gcaggccgat tgacgaccac
    11221 tacgccttct cctgccctgt gccagaaaac ccgcgccagc tttttgctcg cagggccgtt
    11281 gctggctcga gaagggcgcg cggagctgcc ctcaccgggc ggcgacaaga tcggtcggcg
    11341 cccggtcgac acgcacatcg aagctttgcg agagcttggc gcggaggtcg aaattcggcc
    11401 ggatcgttac gtgatgcgcg ccccgaatgg cctggtgggt cgcgatctgt tcctcggtga
    11461 aatgagcgtg atggcgaccg agaacgtgct catggccgcg actctcgcca agggcaccac
    11521 ggtaattagg aacgccgctt cggaacccca tgttcaggac ctctgccgca tgctggtgag
    11581 catgggcgca cgaatcgaag gcatcggctc gaatacgttg accatcgagg ggcagcccaa
    11641 actatcgggg tgcacggccg aggttggtcc cgactatctc gaggtcggaa gcttcatcgc
    11701 cctagccgcg atgacgggcg gacaactctg gattcgtggc gcgtgtccca gccagcaccg
    11761 cgtcacggcc aacgccttcg gcaagctcgg cgtgcgcttc gagagccacg gggacgacat
    11821 cttcgtgccg cgccacagcg agctccgcgt ggaggagggc ctgcacgggg cgctgcccaa
    11881 gattcacgac gcaccttggc cgggtttccc ggcggatctc acgtcaatcg cgttggtgct
    11941 cgcgacccag gctcagggca cgacgctgat ccacgagtgg atgtacgaga gccggctgta
    12001 ctgggtggat cgactgatcg cgatgggagc ccgggtgacc ctctgtgatc cgcatcgcgc
    12061 gatcgtgatt ggccctgcca agctttacgc ccagaacgtc tcttcaccgg atattcgcgc
    12121 gggcatggcg ctcgtgattg cggcgctcgt ggccgagggc aagagcacga tccacaacgc
    12181 gcaacagatc gaccgcggtt acgagcggat cgaggaccgt ttgcgcgcga ttggcgctcg
    12241 catcgagcgc gtcgaagcgc gcaagagttg agctcggaag ggcgcggaga caacggtttg
    12301 gagctggctc gctcaccacg cgccagcttc gccatcctcg agataggcgc cgtccccgag
    12361 tggagtccgt cgcctcggat ccgttcgagc gcaacctagt ttcgagcgca acctagttta
    12421 ggaaccgatc cccgctgccg ttctcgcgat cggtcttctt gccgcccgag atcacccgca
    12481 agtccgaccg ctccacgcgc ttgcgccgat ctctcgcttc acggcgcgct tccgcgtcga
    12541 gctgcgcgag gcgcagcttg agccacagcc gacggaaagg ggaaggcgtg ctgcctccga
    12601 acagccatcc tgccagcatg ccgccgaacg gtgcgatggc gccggcgggc ccccgctcac
    12661 cgatcacgat gtacatgagg ctagccgcga tcacgaacac gatcagaccc gtggaactca
    12721 ccggcagcac gaagaacagc cgaacgacct gcccgcggaa gctcaaggcc caagcaatcg
    12781 cgatcgcctc gaccaccggg atcgcgccaa accaatagtc gtccaccaag cgcgcgcgga
    12841 tcgagtgcgg cagcacgagc tccgcaacca tctgcgtgcc gtacgcgatt aacgcggaca
    12901 cgaacagaaa tcgcgcgaat cgtgcggagc cccagcgttc ttctagcgaa ggcgccaaga
    12961 aatacagacc catcaacgcc gacagcacgt gcccgagggt ttgggtcggc atgtgcagcc
    13021 aaggcgccgt gaagatacgc caaacctcac cgtgcaagat gcgctcggtg ttaccgcaca
    13081 gggcctggaa tagcgaagcc ggtgcgtccg cccaattgat ggcgatcgcg aacgccagcc
    13141 agaccccaaa gatgccgagc atcaccacct tgaggactcg gccaggacga gggaatgcgg
    13201 cctgaagcgg ggtctccggg tagctcacga gtttagggtt gcccgtccta acgcgagccg
    13261 caagccatac tcgaaaccag gcccggttcg acatcggccg tgcactgggt ttggcctatt
    13321 tgcgtgctat cccgcgccca cgcttccacg tccaacacgt gggtaccggg ttcgacggcc
    13381 atgaaggccg cattgccgtt acagctgtgc gagtcgaagg tgagcttgga ctcagtggcg
    13441 tcgagggaga ccacgagccg accgagatcc tcacaacgta cttgtagcgc ttggagcagc
    13501 gcggaaacct ggacgtggat cccgcccacg tcggatagcg gggcgcaggt agcgggcagt
    13561 gtcgtacctg gcgcggtgcg cacttcgcac acgcctccga gcaggggctg ctcagcgctc
    13621 ggcgcgtagg ccaagagctc gagctcatgc accgagtccg acggatccga gaaggtcaag
    13681 gtcaaggttt ctccgcaagc accttcagcc aacacctcgc cctcggaccg aacttccact
    13741 cggtccaccc gcggagtact ggcctcacac tcggcgccga gcacttgagc tacttccagg
    13801 ctcacgcggg cttcagcgcc ggggtcggtg ctcccgccgt tcgaccaagc gctgcaaagt
    13861 aacaccggaa ccgtaagctg atactcggct tgtacggcct cgcaggttgc tttccagcgc
    13921 ggagtaacca gttcgttggt ttcgggatca cgctgggcac ggagccctgg ggcgaacggc
    13981 tcgagaccat cccgatcgta accgtcgagt tccaccgaat agcgatgcag cggcgaaatc
    14041 gcgccgaatg ccaccgccct ctggcaaggc gttgcgctcg agctaggcag gtagaacgaa
    14101 gaaccagggc ggctggcggt ctcgtccttc agcgtggcaa cgtagctcct caacgttccc
    14161 accccagccg cgctgcatgc cagcgcgccg tcgaacgcgg aagggcccac ctcgacgata
    14221 gtggggtgag gttcggtggt ggtggctccc gagctgctgc cgcaagccag cgcgccgaac
    14281 aacggcgcac acgctgcggt ggcggtccaa aaccgagctc tcccagactg agtgggccaa
    14341 gactgagcgg agcataaccg aacggaccgg gacttcacgg gggggagcct tagcccatcg
    14401 ggctcggttc gccaagcacg gaggacgacg tgtttggttc aggtgccagc cgtgccagcc
    14461 ccggcactgc cgccttcgct ggatgggttg ccgctcgagg tgcttcctcc gtcgccgctg
    14521 cttcccgctg cattcgacgt cgttccagcg ccacccccgc tggtttccgg agtgccaccc
    14581 gagccattca cttcgccgcc cgagccgttg agctgcccgc cggtggggtc ctcgctgccc
    14641 gtttcgccgc cgctcgtcga catgttggtt gctcctccgc cgttgccggt gcccgcgctt
    14701 tccacgccgc cgttgccggc ggcggtgccc tcggctccgc cgttgcctcc ctcgcccgtt
    14761 tctgaggggc tcggatcgag gaacgcgcag gcccgcagct tctccgggtg ctcgatggcc
    14821 tcgcacgccg aggccggagt ctgtctcccg gtgcaggcgg atgcggtgtc ttggttggcg
    14881 gccgcgcagg ctccagcggc tcggattgcg gcctcacact cgccgacttg cgtcgcggcc
    14941 gggtctcgcg cgaccaaccc gtgtaggcat tgatcccgat aaaaacggct gcaggcgcta
    15001 gcgtccgaaa tcagcccgca cgcgggtgcg gcttcgcagc gtgcctgttc gatgcgccgg
    15061 caggcgtcga ccccacgagc gtccgttccg caacccggcc cgctcaagag tgcgccgacc
    15121 actgccgaga agagcaggag tgacgcggca cgcagtgact tcatgcgggc gccttagcac
    15181 gccgaaaaac cttggcgcta actttcaccg cttcgagccc tgggtcggca cccggtcgcg
    15241 gcccgactgg caccatctgc gttcagggtc aaactgcgcg agtgccgcgt gcgctggtgc
    15301 tttctgcggg tttcggcagc cgccttcgac cgttgacgga ggagctgcca aaaccgctgc
    15361 tcccgttcgg ggatcgctgc ctgctcgatc acgctttgcg tgcgctggat ggccgtcggc
    15421 tcagcggtga cgtgatggtg aacgtccatc acttggcggg ttgtttcgaa gcttaccggg
    15481 agcgactgag ccgccccgtc gagctgatcc atgagtcgaa cattcgcggc acagccggcg
    15541 gtattgctgg tactcgggag cgcctcgatg gctcgggttt ggtcgtagct atcgcggacg
    15601 tggtgctcga ccaagttccg gaagcgttta tggaagctcc cgcttggggc tcgttggtgc
    15661 tcgcgttgcg gagggcgccc gcggggcgcg gtaacgttgg cgtcgggcag gggggggaag
    15721 tggtgaggct gcggggcgaa cgttttggtg ccgaagcttg gtcggggaac tacgtggggc
    15781 tgtgcgcact cagccaagcc gtcgtggctg acctgcccga cgtggggtgc ctgattggcg
    15841 attacgcttt gcccgtgctt cgcagtggcg gccgagtgtt ggcgttcgag ttcgacgggg
    15901 agtgctcatt tcccggtgac gacttgccgg gctatttggc ggccaacctg gcttggcttc
    15961 agcggagaaa tctcgaacag ttctgtgcgc ccggctcgca gattgcagct ggcgtcgagc
    16021 tcagacgggc cttgctgagt cgcggggccc gagtgagcgg cagtggtcgg ctcgaacggg
    16081 tgatcgcctg tgaagaagcc gagatccagg cccctttgca ggatgcgtgt gtgcttccga
    16141 gcgggagagt ggtcccgatt gctccaccca ctttggctca gtccgaaggc gcgtaatcat
    16201 acgcgcccgg cgcgcgatac ctcagctcga ttgcggggtt aactggctga agttcgagag
    16261 ttctcgagct aattccggac actctttcct cttctggtcg caggcgccgg cggatatcgc
    16321 ggggtagcga gaatcgtaag tcgggttcgt caggcgaccg agttggccgg aaatttcgcc
    16381 tgaccccgcg tgttcggcta tgacttcccc tcgccctcgc gatgcacccc gtgaacgtca
    16441 gaaacctcga gcagatccaa gagcaggata gtgccccgcg gcgctcgcgg atcggcgcga
    16501 ttctgctgtt tgcgggtgct tgtgcagccg tggtcacctc cagcgcgatg atgatcaagc
    16561 ggaaggggcc ggccgcggtt tcaaccgctg atccgctggc ggcgctggtg tccagcgcgc
    16621 gcagcgccac tcccgcggac aagctcgagg gcagggacgt gacgttccca tcggtattga
    16681 gcgatagcga gaggaccacc actgctttgg ctgccgtcaa agacgagcgt ggccgcttgg
    16741 tgcccagggt cgaagctccg cagccggaga atccaccggc tcccgccgag cgtttaccgg
    16801 tcgtgcccct cccggtggga gcgctgctgg gcgagacgcc ggtcacggct gcgcccaagg
    16861 atcgcttgac cgccctagct tcggacgcgc tgcgttccgg agagagccag tcgctcgctc
    16921 cagccggtat ggacggtggc ttccagctgc aagtggcgag cttcaaagag caggccgagg
    16981 cggacaagct ggtggaggaa cttcgacggc gcggtcaccg ggcattccga caagccgcgt
    17041 acgtttctga gcgcgggctc tggcaccgcg tgcgtgtagg gcccttcaag tctcggctcg
    17101 aggcgcagaa gtacaaggcc gagttcgaaa agaacgagcg gctttcacca ttcctggtcg
    17161 atccggagaa ggtgaagcaa gccgaggcga tgcgggccgc gcgtcttgcc gcgcaggata
    17221 aacgcggatc tcgccgccct gtgcctggtg agtgagcgcc tggtgagtga gcgcctggtg
    17281 agtgagcgct ggagcgacgg gcccgccttg ccgcggtgct cgcgtgcgcg cctcggcgcg
    17341 cgactcgcga gagggcctta cgacttgccc tgcttacgca acagctgttg tttggccgcg
    17401 agcgcgattg cgccgctcac gttcttgctc ttcgccagcg agcgcacgtc gttgtcgcgc
    17461 aggtgaggca ttagccgcgc cgcaaaggtg aaaggcgtgc gagggtttgc caccaagttc
    17521 aacttgacct ggtaatgtcg cacgaactcg cggttttgag cgatggcgcg cagcacgtcc
    17581 tccgagacgg agcggctcgc gctgatctgg gtcgcctcgt tttcgcgcat gagcgggctc
    17641 ttgactgccg ctgttgcaac cagccgattc gagtcccgca ccaagaggag gcgctcagcg
    17701 gccgtaccca gcatcgcgcg tcggatcttc tgagtgacac tcagcgtgcc aatgatggcg
    17761 tacagcggca cgaaccgctc ccggagcttt tctgcccctt cgtcgtcgac ctcgtgcgtg
    17821 tcctcgtccg tggcgagcgg cccggctcgc tccgccgtct gctcggtgtc gtgaaacacg
    17881 atgtcgtcgt acgttggctc gggggaggct tcggcgatca gctcgttctt gatggcttgc
    17941 gcgatctcct tgtaggcaga tattgcgacc tcgacgccat tgcgcacggc aagctccagc
    18001 aagcgatccg ccgtagacat gcggacctgc ttgttcatgt agagcttctc gatcaccgtc
    18061 gggttggcaa gcatcagctc ttcgttggtg gcgatgattt cgccgctccg ctcatcggcg
    18121 gcagacgcga gttgctcgag agtgctgaca tccacgctct tttgccgcag taagcgctcc
    18181 agcacggcgt gctggttcaa gtaggcgcgc gctagcttgt ccactacgag cgcgggcagc
    18241 tccgcgttca gtgcgccgtc gagcaacgct tgcggcagct tgccgagcgt cgcggtcgct
    18301 gcctctcgag ccttctcgtc agggctgtcc gtaagtagcg cgacgacgca aaccagttcg
    18361 tggggcttga ctccaggaag cgcaccacgc gccgccatca agcgcagcgg tgcaggcgca
    18421 tccggattca ggatcttctg ggcgacggga gagagcgagc tgaaatcggg gctcgtgagc
    18481 atgggaaacc gcggggcgcg aggtgaaagc atactcggcc tgcatcgtca gcaaaaccga
    18541 gacgcctcga aaaccaaccg ccgagtcgac cgccgctctg gtcgaccgcc gccctggtgc
    18601 tggctcgact gttggtttct ctcgagacta ctgggttccc tcgaggccgc cgtgatagcg
    18661 caccgccttc tcccgcctcc cacccgggaa caggcgcaga tactggattg ctcgatctcg
    18721 cgcgagagtg gcctcaccag cacgtgacca gctctccact tcgcgtgcca acgcgtcttg
    18781 ggacagagcg ccgcccggtg caagcttgcg acaacgtgcg aaagcctccg ctgcttcgcg
    18841 cggccggccc agactatcta gcaagattcg cccgagtgtg aacgcagcca ggggggcgcg
    18901 cgagtcactc ggataggacc gaaccacccg ctccagctga ggcaaggctt gtgcggggtg
    18961 cccgctgagc cgcgccacgt cagccgcgaa caacagatca cccggttctt gccgaatgct
    19021 gcccgcgcct tcttgcgaca acacggcgaa agcccccgcg tagtctccct cttgagcaag
    19081 gctgcgccaa ttcttggagg cggccgcgct acccaacatg ctgttcccgt tcacgctggt
    19141 gcccgcggca gcagttccgc cacccggcgg ggtaataccc ggcgctgcag tattctgcgc
    19201 gccaggattg gcggctccgg cggcgctcgc ggctcccccg ccaactgccg aggactctcc
    19261 cacggtagcc tcttcccccc cagcggtgac ctcttctccc gctcccaaaa cccgacgctc
    19321 ccccggccac tccacctcca cccgaccgcg ttctaccccc acgcgcaccc cgtctgccaa
    19381 cagctcgacc gtgaactgcg tacccagcac ggtcaccgcc acatttcgcg cttcgacgcg
    19441 gaacgtgcgc tcagcccgag gagtaacgtc gaagcgagcc cgcccggagg ccagcctcat
    19501 ggttacacgc tgaacgctct cctcgagagt gagcagatgc gcgtcagccg acatgccttg
    19561 ggccaccgtc ccgtcggtga gctgcaccga cgcgaccccc accgcggctt tgagtgccga
    19621 ccctcgttgt gtttcggcct ccgcgccccc gacgcgttca ccccggccac tggcggctcg
    19681 agcctccgct ccttgatacg ccgcagctcc tcctattccc gttcgtgctc cctttgttgt
    19741 ttgtgctctc tgccccaccg cacccttcgg tgccccatcg atccactgtg aataccccag
    19801 atgccccacg attagcgcca ccaccagcgc catacctgcg aggacgcgcc cggtggtgcg
    19861 ccgtcgctcg agccgactct cgagctgcca tcgcagggat cgctcgcgag ccgcagtcca
    19921 acgtggactc acgtgttcgc gcgcgctctc gatccgctct agaaagtccg tcattccgcg
    19981 aacgcctcct cgagcagtcg agatactgct tggatgcggc gtttagccgt cgctagcgag
    20041 cacccgcaca gctcagcgac ggcctccagt tgttctcctt cgatgaaacg aagggtccag
    20101 gcgatgcgct gattggtggg tatcccatcc agcactttgt aaacacgcgc caacaaggca
    20161 cgttgctccg ggctggctcg gtcgtccgca accgcttggt aagcggccgg tgtatccaag
    20221 ccgagaaagc tgcgcattcg gcgcatccgc aacttgcgtc gcgccgcacg cactgccact
    20281 cgcgccagcc aggctttgac ggcgagtgga tcgcgcagct gcgagatccc tctcaccgct
    20341 gcgatgaaaa cctcctgcac ggtatcatca acctctgcgt cctgacccag aaggcggtgg
    20401 gccacggccg ctacgtaagg agaataacgt cgatacaagg tgtcgaaatc gagctgcagc
    20461 tccagctcca cctcaccggg catcaccacc tcactgggga tctccacctc gccgggaggc
    20521 acctccactc gttcgtgagg cgctgccacc gaggggacga cctgcagtgc ggcgtgggct
    20581 cgttgcatcg cgtttcccct cgtgagtgtg catcaagaat cgaaacggct cacggtcttt
    20641 gattttgagc cgaagtacct ggagctcgcc cactcacgcg gtgcatgccg accttaccgt
    20701 tgcccccgcc ggcaccgcgt gtctcgctct gcgccatgat tcggcccgtt cgctgctggc
    20761 ccgcgctgct agcctgctca gtggacgcct aacttatcgt cagccgcgcc gctcgtttcg
    20821 tcgctcggct cgccgaactg ctccagactt cgtgcggatt gggactcact cgagtggtcc
    20881 gggcgagcgt ggagcttgcg cgctcagagc tcggtccgag ctaagcaagc ttcgccgtgt
    20941 ttgcctcgaa tcaggggata aagccaatgc cccactgcgt tccgacccaa attccgctga
    21001 tcggggtgat agtgcctccg gctacgggaa tggccgcgta gctaccttct gcggagacgt
    21061 tcaacacgac cggtccgctg cgtgccgaca atgacagcac gagcagaggc caaccccacg
    21121 gcaccaaaac gtttttggcc ctgcccaagt ccctatccag cgactcggct ttgacggcgc
    21181 caacgcgcaa gccggcgccc gcgcgcgccg tgacgatacc ggttcgatag tacaaaagta
    21241 ggctggccgc gaaggaccaa gtctgaatgc gataccgaag gtcttgcttg cccgtgagcg
    21301 tgccgccctc ataaagagca tctacggacc agctggtcgc gcgcagcgcc tcttcgctca
    21361 cccgcactcc agcgccccaa agttgaccgg ggtgctgtag gaaagcccgc accgaaccga
    21421 gcgccgagag gtgaaacatc cgctcttcgc gcggtagcgg ggcgtaccaa ggtctgctca
    21481 tcggcgctgc aggcagtgga gtctccgcgt ccgcgacgcg gacgccggtg cccgcctcga
    21541 gccgcgccag caccgcgtgc acgtctttct ccgaagccac cggaccaagt ggttgaaccc
    21601 gcaggcttgg ttggtacgtc agctccgccc aactcgcgag aacgagttcg gccgcggcga
    21661 tggcgaccaa gcgagcgcgc gctcgtggtg gaagcgaacc ttcatcgaaa gtgcgtagga
    21721 gcgtctt
//
LOCUS       DASTKU010000924         8581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1522002, whole genome shotgun sequence.
ACCESSION   DASTKU010000924 DASTKU010000000
VERSION     DASTKU010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8581
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522002"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..2227
                     /locus_tag="VFQ61_32640"
     CDS             365..2227
                     /locus_tag="VFQ61_32640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HET9959299.1"
                     /translation="MKFRRLSSWSLFGLGGLLAFTSARTAGAVIVSEGHIVEAWPEAA
                     AAAHALPPGVALAQVGGFFPQRKGTIRGLTLLVNFSDEPGAFTREEIEEWLNAPGYSR
                     FNCNGSVRDYYKDVSNGAVDLVNTVHAYYRAKNPKSYYEGGNGYERAGELMKELLAAV
                     DPAVDFSQFDNDGDGRTEAISVVYAGPSVEWGQGLWPHSGSLSEKRDGVSLPRYQMTN
                     MGKTLSLYTFVHETGHMVFGWPDLYGFGDYCVMGNATNPVNPAGINDFFRADQGWIPT
                     VDVVAATNASYIATPNGAGYRFMNPNNSKELFFWSNIQPKNRWSVLRGGGLLLLHYDG
                     TIRGNDPPNPLQLAVVQADGKKDLDKTTWPSPGSDSNDFYRSGGRSEISATTTPNSSW
                     NNGSASGLRVHSVSASGNEMSFSVGTGVAGAPSASGGTTSGGASSGGAGSGGRNNGGA
                     TNGGRNGGGANSGGASNGGATGGAAGGSSARGGAPSQGGSPQLGGSSSVPGAGGGGNN
                     SGTGGVLNSAGMFGAGGATASGGSFASAGGGSSVAGMSARGGAANEGNSSVGAGGATT
                     AGSPPRTEPPADSSGWSCTTAGHAGQRGTWLGVACLASVLTWTRRRSRARTREA"
     gene            complement(2343..4784)
                     /locus_tag="VFQ61_32645"
     CDS             complement(2343..4784)
                     /locus_tag="VFQ61_32645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959300.1"
                     /translation="MERLAMSVLVRGLLGVGTTGVAIHCAACSNREDATAADRSSGGE
                     TQTQGGSTGTLIPLPTGGGTVKTQNQHLVVTPESEVATVKIGESPSPISLTATLDGVP
                     VNAMWSVDYGNIGVVRPRAGSRVTFTPSGTTAGSVQITASTSSASGMAKLSVRLVGAQ
                     NGANIHVAGEAAQIAGSLGDLTLGGGIGGVGGEGLGPAVTDGDVAALANPQGDGAREQ
                     LSLLYPYDRTVWPRGMLAPLLMWKWSVGDADAIRIDLSTVSGNFSWSGTFGRPAILES
                     LAPTGTGSSTESSGRFIRHPIPQDIWEIATNSATSSDPLQLELSVARDGKAYGPVRRI
                     WPVAPGRLTGTVYYGSYATALVENSGQQRRKGGGAYGSGVLAIRSGETRPRLIAGQPS
                     TDDKGCRGCHMVAANGSRLIAQTTRQYYGGTATYDLLRGNQETQLGVEWDKVFAWAAL
                     SPDGTLAFTSQLRFGVAEVGPPPPASRLYAFPPEPGALPIPVEGLSSDVQVGTPAFSP
                     DGKRLAFYLMGGMAGAISGTGNELVAGDFDRSAMAFSNARLLASTADPERAGFPSFLP
                     TGDALLFHHQLRPLASVPLLPTGGVEQEARLDWSDLDTGTSAPLHTLNGARPDGSSYL
                     PTSDLHPDDTLLNFQPNVNPVSTGGYFWVVFTSRRLYGNLAESHPWYSDPGRYDLTDY
                     SDIPLKKLWVAAIDMGSYVNGQFVPGNAPGSDPSHPAFYLPAQELMAGNSRGYWVLDP
                     CKNNGAACESGDQCCEGYCRPSGDGGTLVCAGAPPTSQCAQVQEACEKSADCCDAANV
                     CVNRFCAFVSPEPPR"
     gene            5223..>8581
                     /locus_tag="VFQ61_32650"
     CDS             5223..>8581
                     /locus_tag="VFQ61_32650"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HET9959301.1"
                     /translation="MKTSYLWILAALGSLMGCGSGQELSNREDTGELTNALVTSVSPS
                     PGLRRVLVLSIDGMHAVDLYKWVTNNPRSNIARLYQHGVDYPNATTTTPSGGLPGMLA
                     LATGGTPRTTGVYHEDSYDRTLYPPGSNCVGGVGTEVVYDASVAQDSTLLMSAIEPTN
                     LPLRVNSQGACVPVYPHEFLRASTVFEAVQGSGRRTSWTDNHAAYEILKGPSGNGLSE
                     FFPLEVGSLVVNGGTVNGVNLSNSSLLCNATNSITTVLDYTNCLPAAQAYDDVKVQSL
                     INKIDGFTADGARQAAVPTLLGMNFVAVEVAQKLPMGGYVDAIGNPSSLLLKAFQRVD
                     LSIGRILTELNTKHLIEKTLVVLTAKHGQAPIDKDLLWTEVSGPGGIPGVINPLALVQ
                     TADPNVDSVFASFVNPNNGRSPAVNGHVQTGGDVGLVWLQDQNNVNVSNVVQVLSDPL
                     NGEAMGATERPQGSIFSANIASGTELSETYGDPQSSDALAAARAPNVLVQPNAGVVYS
                     SGPFIADHGGGAIEDINVPLLVSNTAIARHLSIYEPVSTTQVAPTILKALALNGVKLT
                     AAIKEQTNTLPGISWGAGQYQAGSTIKAVDYDSQTNTQTDGTAPPFVSYFDSGSSICF
                     DDVSLDNVVAIQATLASENANGTFSIRINGPNGTQIGSYAVQPTGGWTNWSTVTIPIT
                     ATTGTHTLCFRGESSSGIANLLSFYLVGTCIPECTGAACGSDGCGGTCGACAFNFICD
                     DPYAQCVVPPTRYSLVDPALPYVIPAPAYDVQTGTTPGGDGSVGAFDANDYLCYYGVD
                     LTSVVSLVTSFGAANAGGVFSIRIDSPTGTQIGSYTVPSTGGATNWVSKRVALTTPVA
                     GVHAVCIRGQSGTSIARFRSFELSSLPVLPKGTVGSTVSAVPPDLQQGTTIEAPGHVA
                     NFDANDYFCYDDIDLTGVNSISVNVASSNGGGAVSVRVGSPTGTQIGSFTMPNTGGWT
                     NWVDMNVGISSSPGMKTLCFFGVSGNGIGNLRTFTLSATPVTAHYALGSTFPAAPPDQ
                     LQGVTSSGGIISNFDGSDWFCYQQVDLTGVKAVVLRAGSANNGGQISVRIGIPAGLQI
                     GSYTAWNTGGWSNWQDYRIGISSTATTDTLC"
BASE COUNT         1645 a         2636 c         2749 g         1551 t
ORIGIN      
        1 tcgcggctca gggaaacgca ggctcacccg cccggttcgc gcaagtcgtg cgcagaaaga
       61 gccccgaaat ccgagctgat catggcggct gtcaggttga cgccgctgtt cgctggggcc
      121 acgccaaccc gcagggtccg ctagccgtca ccggccgctg atacgaaagg cgtggtctcg
      181 agccccttgt cagaataccc gcgagacacc gacggagtcg ctctgttcgt gctctcagcg
      241 aggcgttggt ccgaatttta tgctgtgggc cgttcggcga gccaaaaaac agctgcgttg
      301 cgccccctcg cagctggctc gcgacactta ctgacgtgtt gcgacgcgcg cgaaagggcc
      361 tctcatgaag tttcgacgtc tctcgagctg gtctctgttt ggcctcggtg ggttgctcgc
      421 gttcacgagc gctcgcaccg cgggggccgt gatcgtgtcc gaggggcaca tcgtcgaagc
      481 ttggccagag gcggcggcgg cagcgcacgc gctgccgccg ggtgtcgcct tggcgcaggt
      541 cggcggcttc ttcccgcagc gcaagggcac gattcgcggc ctcacgctgc tcgtgaactt
      601 cagcgacgaa cccggcgcgt tcacgcgcga ggagatcgaa gagtggttga acgcgccggg
      661 ctactcgcgc ttcaactgca acggctccgt gcgcgactat tacaaggacg tatcgaatgg
      721 tgccgtggat ctggtcaaca ccgttcacgc ttactatcgc gcgaagaacc ccaagagcta
      781 ctacgagggg ggcaatggct acgaacgcgc gggcgagctc atgaaggagc tgctcgcggc
      841 cgtcgatccc gcagtcgact tctcgcagtt cgataacgac ggtgacggcc gaacggaggc
      901 gatcagcgtg gtgtacgcgg gaccctcggt cgagtgggga caggggctgt ggccgcactc
      961 gggctcgctc agcgagaagc gcgacggcgt cagcttgcct cggtaccaga tgacgaacat
     1021 ggggaagaca ttgtcgctct acaccttcgt gcacgaaacc gggcacatgg tgtttggctg
     1081 gcccgatctg tacgggttcg gcgattactg cgtgatgggc aacgccacca acccggtgaa
     1141 ccctgcgggc atcaacgact tcttccgcgc cgaccagggc tggatcccga cggtcgacgt
     1201 cgtggcggcg accaacgcga gttacattgc cactcccaac ggcgcgggct accgcttcat
     1261 gaatccgaat aactctaagg agttgttctt ttggtccaac attcaaccca agaatcgctg
     1321 gagcgtgctg cgcggcggtg gcctgttgtt gctgcactat gacggcacca tccgcggcaa
     1381 cgatccgccg aatccacttc agcttgcggt ggtgcaagcc gacggcaaga aagatctcga
     1441 caagacgacg tggccctcac ccgggagtga cagcaacgac ttctatcgat ccgggggccg
     1501 cagcgaaatc tcggcgacca ccacccccaa ctcgagttgg aacaacggca gcgcgtctgg
     1561 gctccgcgtg cactcggtta gcgcgagtgg caacgaaatg agcttcagtg tcggtacggg
     1621 cgtcgccggt gccccgagcg ccagcggcgg cacgaccagc ggcggtgcca gtagcggcgg
     1681 tgccggtagc ggcggcagga acaatggtgg cgcgaccaac ggcggtagaa acggcggcgg
     1741 cgcgaatagc ggcggcgcca gcaatggggg tgcaaccggt ggcgccgcag gcggcagcag
     1801 cgcgcgcggt ggagcgccaa gccaaggcgg ctcaccgcaa ctcgggggca gctcgagcgt
     1861 gcctggtgcg ggcggcggtg gaaacaactc gggcacgggc ggcgtgctca actcggcggg
     1921 catgttcggt gcgggcggag ccacggccag tggtggaagc tttgcctctg ccggcggggg
     1981 ctcatcggtc gcgggcatga gcgcccgggg cggtgccgcg aacgagggca actccagcgt
     2041 tggagccggc ggcgcgacga cagccggctc gccgcctcgc actgagcctc ccgcggacag
     2101 ctccgggtgg agctgcacca ccgccgggca cgcgggtcag cgaggcacct ggctcggcgt
     2161 agcgtgtttg gcctcggtgc ttacctggac gcgccggcgc tcgcgcgctc gtacgcgcga
     2221 ggcatgacga cttcgcagtc gtcccgactc cgcgtttcag ctcgaaaagg agctgcggcg
     2281 gcgctcgtcg taggcggact cgtctggccg agcccttcat gcgagcgacg ctgtcctccg
     2341 catcagcgcg ggggctccgg gctgacgaag gcgcagaagc ggttcacaca gacgttcgcg
     2401 gcgtcgcaac agtcggcgct cttttcgcag gcttcttgaa cttgggcgca ttgggaagtg
     2461 gggggcgcgc cggcgcagac cagggtgccg ccatccccac ttggtcggca atatccctcg
     2521 caacattggt cacccgactc gcacgcagcg ccgttattct tacacgggtc gagtacccag
     2581 tagccgcgcg agttccccgc catgagctct tgagcgggta gatagaacgc gggatgactc
     2641 gggtcagagc ctggcgcgtt gccgggcacg aattggccat tgacgtaaga gcccatatcg
     2701 atggcggcga cccaaagctt cttcagagga atgtccgagt agtccgtgag gtcgtagcgg
     2761 ccggggtcgc tgtaccaagg atggctctcc gccagattgc cgtacaggcg ccggctcgtg
     2821 aagacgaccc agaaatagcc gccggtggac accgggttga cgttgggctg gaagttcagg
     2881 agtgtgtcgt cgggatgcaa gtcgctggtg ggcaggtatg acgaaccatc tgggcgagca
     2941 ccgttcaacg tatggagcgg agctgaagtt cccgtatcca gatcggacca atccaggcga
     3001 gcttcctgct cgaccccgcc cgtcgggagc aacggtacgg acgcgagtgg ccgcagctgg
     3061 tggtgaaaga gaagcgcgtc gcccgtaggg aggaacgacg gaaatccagc ccgctcggga
     3121 tccgccgtgc tcgcgagtaa acgagcattg gagaaggcca tcgcgcttcg atcgaagtcg
     3181 cccgcgacga gctcattgcc agtccccgag atagcaccgg ccatgccgcc catcaggtag
     3241 aaggcaaggc gtttcccatc cggtgagaat gcgggagttc ccacttgaac atccgatgac
     3301 aatccttcga ccggaatagg aagagccccc ggttcgggag gaaaggcata caaccgactc
     3361 gccggggggg gagggccgac ttcggcaact ccgaatctca attggctggt gaaggccaga
     3421 gtcccgtcgg gcgaaagagc agcccaggcg aagaccttgt cccactcgac accgagttgc
     3481 gtctcctggt tgcctcgcag cagatcgtaa gtcgcggtgc cgccataata ctgcctggta
     3541 gtttgggcaa tcaggcggct cccgttcgcg gccaccatat gacagccccg gcatccctta
     3601 tcgtcggtgg atggctggcc cgcgatcaag cgcggccggg tctcgcccga acggatggcg
     3661 agaacgccgg aaccgtaggc cccaccgcct tttctccttt gctgccctga gttctcgacg
     3721 agggccgtgg cataggagcc gtagtagacg gttccagtca ggcggcccgg cgcgaccggc
     3781 caaatacgcc taacgggtcc gtaagctttg ccgtcgcgag ccaccgagag ctccagctgc
     3841 aaaggatcgg acgaggtggc gctgttagta gcgatctccc agatatcttg cgggattggg
     3901 tgtcggatga atcgacctga actctcggtg gaagagcccg tgcccgtcgg ggcgagtgac
     3961 tccagaatcg ctgggcgacc aaacgttcct gaccaagaaa aattgccaga gacggtggag
     4021 agatcaattc gaatggcgtc cgcgtcaccg acgctccact tccacatcaa cagcggcgcg
     4081 agcatgccgc gcggccacac ggtccgatcg tatggataaa ggagagagag ctgctcgcgc
     4141 gcgccatccc cttgtgggtt tgcgagagca gcgacgtctc cgtccgttac ggcagggccg
     4201 agcccctcgc ctcccactcc gccgattccg ccgccgagcg tcaaatcccc aaggctgcct
     4261 gcgatctgag cggcctctcc cgctacgtgg atgttggctc cgttctgggc gcccaccaaa
     4321 cgcaccgaga gcttcgccat gcccgaagcg ctgctcgtac tcgcagtgat ctggaccgag
     4381 cccgcggtgg tccccgacgg agtgaaggtg actcgacttc cggctcgcgg ccgaacgaca
     4441 cctatattgc cataatcgac actccacatc gcatttacgg gcacaccatc caaagtggca
     4501 gtcaagctga tggggctcgg gctttcgcca atctttacgg tggcaacttc ggactcgggt
     4561 gtgaccacga gatgctgatt ttgcgtcttc acggtgccgc cgccggtcgg cagcgggatg
     4621 agcgtgcccg tcgacccacc ctgggtctga gtctctccgc cggaactgcg atcggcggca
     4681 gtcgcgtcct cacggttcga gcacgccgcg cagtggatcg ctactcccgt cgtgccgact
     4741 ccgagcaacc ctcggacaag aacactcatc gctaggcgct ccatgccccg aatcctaagc
     4801 tttcgaggga gggcgcagca agcaacgtga ataaggttcc ggcgaatggg aacctcgcat
     4861 tcaggctcac acatcggaga atgactggga ggttcccgag ttcttggcta gtcacatgac
     4921 tactcagctc gagatgcgct cgcggtgttg cttgccgcct tcggtcctcc tctccattgc
     4981 ggaacgggac atcaacctcc gacgtgacgt tacctccggg cgcggctcag cgccgacagc
     5041 gacgcttcac tgagagaaaa aaacgagctg cggtaacaac gaatagcctt tgtcataaat
     5101 cgatatcgag agtcctgttc agagaaactc cgtttgtggt atttacgccg tcacgctgag
     5161 tcgggcgtga aggtcgcgct cgttccaacg ggtcggtggg aacgcgaacg ggatggcgga
     5221 acatgaagac gagttatctc tggattctgg cggccctggg gtcactcatg ggctgcggtt
     5281 cgggccaaga gctctctaat cgggaggata ccggtgagct aaccaatgcg ctggtgacct
     5341 cggtgagtcc gagcccaggc ctgcggcgcg tgttagtcct gagtatcgat ggcatgcacg
     5401 ccgtcgatct gtacaagtgg gtgaccaata atccacgctc gaatatcgcg cggctgtatc
     5461 aacatggggt ggattacccg aacgccacga cgacgacgcc ttccgggggg cttcccggga
     5521 tgctggcgct agctacgggg ggtacgccga ggacaaccgg ggtctatcac gaggatagct
     5581 acgaccgcac tctctatccg ccaggcagca actgtgttgg cggcgtgggc accgaagtgg
     5641 tctatgacgc ttcagtggcg caggattcga cgctgttgat gagcgcgatc gagccgacta
     5701 acctgccgtt gagagtcaac tcgcaggggg cctgcgtgcc ggtctacccg cacgaatttc
     5761 tgagggccag caccgtcttc gaggcagtgc agggctcggg ccggcgcact tcttggacag
     5821 acaatcacgc cgcttacgag atcctgaaag gcccttcggg gaacgggctc tccgagttct
     5881 ttcctttgga agttggttct ctcgtcgtca acggcgggac cgtcaatggc gtgaatctat
     5941 cgaacagcag cctgctctgt aacgctacga attcgataac gacggttctc gactacacga
     6001 actgtctgcc cgcagctcag gcctacgacg acgtcaaagt tcaatctttg atcaacaaga
     6061 tcgacgggtt tacggcggac ggcgcgaggc aggcggcggt tcctacgctg ctcggcatga
     6121 acttcgtggc ggtcgaggtt gcccagaagc tgcccatggg gggctacgtc gacgcgatcg
     6181 gcaacccaag tagcctgctg ctgaaggcct ttcagcgcgt cgatctctcg attggtcgaa
     6241 tcctcaccga gctaaacacc aaacacctga tcgaaaagac actcgtggtg ttgacggcca
     6301 agcacggtca ggcccccatc gataaagatc tactgtggac cgaggtctca ggcccgggcg
     6361 gaattcccgg ggtcatcaat ccgctggcct tggttcaaac cgccgatccc aacgtagaca
     6421 gcgtattcgc gagcttcgta aatccaaaca atgggcgcag cccggccgta aatggccacg
     6481 tccagacggg tggtgacgtc gggctcgtgt ggctgcaaga ccagaacaac gtgaatgtct
     6541 cgaacgttgt ccaggttttg agtgacccgt tgaatggcga agccatgggc gccacggagc
     6601 ggccgcaagg ctctatcttt tcggcgaaca tcgcgagcgg caccgagctg agtgagacat
     6661 atggcgaccc ccagtcgagc gacgcgctag ccgccgcgcg ggcacccaac gtgctggttc
     6721 agcccaacgc gggcgtcgtt tacagttcgg gccccttcat tgccgaccac ggcgggggag
     6781 caatcgagga catcaacgtc ccgttgctcg tctccaatac cgcgattgcg cggcacctct
     6841 cgatctacga gccggtctcg accacccaag tcgcgccaac catcctcaag gcgctcgcgc
     6901 tgaatggcgt caagctgacg gcggccatca aggagcaaac caacacgctg ccaggtatct
     6961 cctggggcgc aggccagtat caggccggga gcacgatcaa agccgtagac tacgatagtc
     7021 agaccaatac tcagacggac ggcaccgcgc cgcctttcgt cagctacttc gactccggca
     7081 gctcgatctg cttcgacgat gtctccctgg acaacgtggt agcgatccag gcgacgctcg
     7141 cgagtgagaa cgcgaacggt accttcagta tccggatcaa cgggcccaac ggtactcaaa
     7201 ttggcagcta cgcagtccag ccgacgggag gctggaccaa ctggagtacc gtcaccattc
     7261 cgatcacagc gacgaccgga acccacacgt tgtgcttccg cggcgagagc agcagcggga
     7321 ttgccaatct tttgagcttc tacctggttg gcacctgcat cccggaatgc acgggcgcgg
     7381 cgtgcggctc ggacggctgc ggcggcacct gtggagcctg cgcgttcaat ttcatctgcg
     7441 acgatcccta tgcccaatgt gtggtgccgc ccacccgata ctcgctggtg gatccggcgc
     7501 tgccctacgt gattcccgca cctgcctacg acgttcaaac cggcaccacg ccgggcggag
     7561 acggcagcgt gggtgctttc gatgccaacg actatctctg ctattacggc gtggatctca
     7621 ccagcgtggt ttcgctcgtg acctcgttcg gagccgccaa cgcaggtggc gtattctcga
     7681 ttcggataga tagcccgacg ggtactcaaa tcggcagtta cacggtgccg tcgacgggag
     7741 gcgccacgaa ttgggtgtcc aagagggtcg ccctcacgac tccggtggcc ggggtccacg
     7801 cggtttgcat ccgcggtcag agtgggacga gtatcgcccg cttcagaagc ttcgagctct
     7861 cttccttgcc ggtgctcccg aagggcacgg ttggaagcac ggtgagtgcc gttcccccgg
     7921 acctgcagca gggaaccacc atcgaagcgc cgggacatgt cgccaacttc gatgcaaacg
     7981 attacttctg ctacgacgat atcgatctca cgggtgttaa ctcaatcagc gtcaatgtgg
     8041 ctagtagcaa tggtggaggt gcggtttcgg tccgagtggg gtcgcccacg ggcactcaaa
     8101 tcggatcgtt cacgatgccc aacaccggtg gctggaccaa ttgggtggac atgaatgtcg
     8161 ggatctcctc gagcccgggc atgaagaccc tctgcttctt cggtgtatcc gggaatggga
     8221 tcggaaacct gcggaccttc actctctctg cgacgccggt caccgcgcac tacgcgctgg
     8281 ggagcacgtt ccctgcggct cctccggatc aattgcaggg ggttacgagc agcggtggca
     8341 tcatcagcaa cttcgatggt tccgattggt tctgctacca gcaggtcgat ctgacgggcg
     8401 tgaaagcggt cgtgctgagg gcgggcagcg ccaacaatgg cgggcaaatt tcggtacgta
     8461 tcggcatccc cgcgggcttg caaatcggca gctacaccgc ctggaacact ggtggttggt
     8521 ccaattggca agattatcgg attggaatca gcagtaccgc gaccacggac acgctatgta
     8581 t
//
LOCUS       DASTKU010000925         6075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_849508, whole genome shotgun sequence.
ACCESSION   DASTKU010000925 DASTKU010000000
VERSION     DASTKU010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849508"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VFQ61_32655"
     CDS             <1..830
                     /locus_tag="VFQ61_32655"
                     /inference="COORDINATES: protein motif:HMM:NF015683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HET9959302.1"
                     /translation="PGGFSEGSFFTARMAELFPALRRAGDPGQAWCQALARSAPPGAH
                     FAFISAPGYLQDHQVVAYLATGLRERGISSWIGKPEQLDWQQGEAFWLPARDRQPLAA
                     IIRFYQAEWLAELPARSAWQHLLRGGRTLVANPIQALISESKRFPVLWDQLTTSLATW
                     RALLPESRDPRGFNGASREWLLKQAYSNNGDAVILPENEPDWRWARLKARLRPNTWVA
                     QRRFESLPFPTPIGPRHACVGVYTVNGQATGAYVRLSSDPVVDYRAIDAALLLMNDD"
     gene            823..1569
                     /locus_tag="VFQ61_32660"
     CDS             823..1569
                     /locus_tag="VFQ61_32660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959303.1"
                     /translation="MTNEQIFSAWAPDSSSWSRWAKPLLFAHIEGDPIPRRPRSSHQD
                     ASWCPPADGRFALILDLPCADGVWFGIRLAEHGYRLVPLYNSLPSTTPRVRSAVNVRP
                     ILRALRDAALILQTLQIPSNAPPAFLLDSRRQGRSFGPQDFDNRAYSFTTDFPSANHL
                     LSQGIQNILLIQPTDAEPQSDLLHTLRAFQDGGLPLALKNLQSPAPPGPFNAPKPPWY
                     RLAFQRALDTLGFHRAPQGGFGAIPRSSSG"
     gene            complement(1879..2256)
                     /locus_tag="VFQ61_32665"
     CDS             complement(1879..2256)
                     /locus_tag="VFQ61_32665"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HET9959304.1"
                     /translation="MESTFCVEALHEALERFSKPGIFNTDQRSQFTAEAFTKVLRDAD
                     IAISMDGRGRCIDNVFAERVWRSLKYEEVFLHAYDDLGEARAGIARYFEFYNFTRQHQ
                     AIDVFLMIANPSRRPPYATLLLV"
     gene            3224..3787
                     /locus_tag="VFQ61_32670"
     CDS             3224..3787
                     /locus_tag="VFQ61_32670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959305.1"
                     /translation="MTVRVGVGCLRKQVVFAVIRRAIARVNGMREDRFRVVEFSVQAN
                     HVHFIVEAEGRTELSCGMRALNARIAKQVNRVLGRRGQLMSERHHEQPLRCPRAVRHA
                     LCYVLANFRKHATHEGLGGEPLDICSSARYFGGFLELNSTTSRETSGPRRARGAPVAS
                     ARTWLLSVGWKKHGLISIHEGPRGAAV"
     gene            4404..5132
                     /locus_tag="VFQ61_32675"
     CDS             4404..5132
                     /locus_tag="VFQ61_32675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959306.1"
                     /translation="MTSKRTAIVLGGSGSVGMALLRELFQDDGFEVVITLSRRTLPEA
                     VALARASGRRLLEKLVPEMTPALLAAATLEAAKDVEGDLEGFSVLGVGAGTAKLTLDE
                     HRAVDVALNEAFARALRDSGKVRHFAFMTAVGADPNAKASGGGAAGMSRYNRVKGEAE
                     EAVRGAGPSIVSVFRPAMIIGSQHTPWLLEKALPLFSFVTPGKFKSITVAQIAKAMVA
                     TAKHHPAASATYHYPEMMALITSC"
     gene            complement(5290..5820)
                     /locus_tag="VFQ61_32680"
     CDS             complement(5290..5820)
                     /locus_tag="VFQ61_32680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959307.1"
                     /translation="MKLILKQALFALVVTGAAFSPKFARADEMTESCSPLTPAAISTL
                     IATLEDSVAQANADCASFCEGGVYAAAAKTNLDGLNDALGQILGLQTWLVENGLDSPY
                     VTNASAAYGIHGVARETLYLLHHARHWATVSAVYHHSAAARLSIDLTTQAIHQLEALG
                     EAGGLCYLSGYGPYTS"
BASE COUNT         1143 a         1951 c         1854 g         1127 t
ORIGIN      
        1 ttcccggcgg cttcagcgaa ggctccttct tcacggcgcg catggccgag ctcttccccg
       61 cactccgccg cgcgggcgat ccgggacaag cctggtgcca ggcattggcc cgctccgccc
      121 cgcccggggc acacttcgcc ttcatctctg ctcccggcta cctccaagat caccaagtcg
      181 tcgcctacct cgccaccgga ctgcgcgagc gcggcatctc cagctggatt ggcaaacccg
      241 aacaactcga ttggcagcaa ggcgaagcct tctggctccc cgcgcgagac cgccaaccct
      301 tagcggcgat catccgcttt taccaggcgg agtggctcgc agaactgccc gcccgcagcg
      361 cctggcaaca cctcctgcgc gggggccgaa cactcgtagc gaaccccatt caggccctca
      421 tatccgagag caaacgcttc ccggtattgt gggaccaact caccacctca cttgccacct
      481 ggcgggccct acttccggag agtcgagacc cccgcggctt caacggggcc agccgcgaat
      541 ggttactcaa acaggcgtac tccaataacg gagatgccgt catcctccct gaaaacgaac
      601 ccgactggcg ctgggcacgc ctcaaagccc gcctccgccc caacacctgg gtcgcccaac
      661 gccgcttcga atccctcccg ttccccactc caattggccc gcgccacgcc tgcgtcggcg
      721 tctacaccgt aaacggccaa gcaacgggag cctacgttcg tttatccagc gaccccgtcg
      781 tcgactatcg agccatcgat gccgccctac tcctcatgaa cgatgactaa cgagcaaatc
      841 ttcagcgcct gggcgcccga ttcctccagc tggtcccgct gggccaagcc gctactcttc
      901 gcccacatcg aaggcgatcc catcccgcgg cgcccgcgct catcccacca agacgcgagc
      961 tggtgtccgc cggccgacgg ccgcttcgcc ctcatcctcg acctaccctg cgccgacggc
     1021 gtttggttcg gaatccgact cgcagagcac ggctatcgcc ttgttcccct atacaactca
     1081 ctcccatcca ccacccctcg agttcgctca gccgtcaacg ttcgccccat ccttcgtgca
     1141 cttcgcgacg ccgccctcat cctccaaact ctccaaatcc cgtccaacgc accgccggcc
     1201 tttctactcg actctcgacg tcaaggccgc agctttggcc cccaagactt cgacaaccgc
     1261 gcctactcct tcaccaccga cttcccctcg gccaaccacc tcctatctca aggcatccaa
     1321 aacatcctcc tcatccaacc caccgatgcg gagccccaat ccgacctcct ccacaccctc
     1381 cgcgccttcc aagacggcgg cctccccctc gccctcaaaa atctccaaag ccctgcaccg
     1441 cccggcccct tcaatgcccc caagcccccc tggtatcgcc tagcctttca acgcgccctc
     1501 gacaccctcg gcttccaccg cgccccgcaa ggcggcttcg gcgccatccc ccgctcttcc
     1561 agcggctagc cccaacttcc ctcgccatcc cctagaattg gggactaggc cacgaagcaa
     1621 cgagcgcgcc cccagtccca ttcgcccacc cgcgcagcca caatcccccg cagcttctcg
     1681 caacaccgcg caggcccccg cgacaacaga cgtcacttca cggcggagtt cctctcgccg
     1741 tcaaacgtgg cgctcacgag tgttaggatc ggtcaacgtc gttcgacgcc atcgcgatat
     1801 gccttgtgtg agccgcgcgc ccgcaatcga acggcagagc gccgcgcttc tgagcgggag
     1861 agacgtggtc cccaccagtc agaccaaaag caaggtggcg taaggtggac gcctgcttgg
     1921 attggcaatc atcaaaaaca catcgatagc ctgatgctgc cgcgtgaagt tgtagaactc
     1981 aaagtagcga gcgatgcctg ctcgcgcctc gccgaggtcg tcataggcgt gcaggaaaac
     2041 ttcctcgtac ttgagcgagc gccagacgcg ttcggcgaag acgttgtcga tgcagcgccc
     2101 tctgccatcc atgctgatcg cgatgtcggc gtctcgcagg accttggtga acgcctccgc
     2161 agtgaattgt gagcgctggt cagtgttgaa gatcccgggc ttggagaagc gctccagcgc
     2221 ctcgtgcaac gcctcgacgc agaacgtcga ctccaacgta ttggacagtg gtcgaaggtt
     2281 tcggtgccac cgactcgagc cccatcactc gcatcagagg cgtcgcatac gcttgcgatt
     2341 gacgtttatg cgcgagttcc tcggcgctgg tgtcgacggg ctcgtagtac agccccgagc
     2401 ggctgacagc gagtagctca cattggcggc gcagcgaaac ggtctcctgc acctcgatcc
     2461 tggctcgacg ctgagctagc ggtgtcgcgc gagcccgcgc gctaaaaaat cgcgctcgac
     2521 ggtgagctcc ccgattttcc agcagctcct tctcgcgctc cgggctgccc gtcgcggcct
     2581 ctgcagggtg ttcgaacacc gacgcagcgc ggtccagaaa ctccttcttt cacttgtaga
     2641 tctggttggc gtgaacgccg tgccgccggg cgagctcggg gatagtctcc tgctcgcgaa
     2701 tggccgttag cgttgaactg gtcctaatct ggggaccact tcaaatatcg attttcgagt
     2761 tcgtgagttc cggcacttgg cggcggagtg agcgttcggg acgtcggggt tattctgggg
     2821 gccggcgcgg tgttgggggg cggctcgagc gccggggcgg cgctctcggg tataaaagac
     2881 gagcttcgcg cacgaaaaca tcgtggtcac caaacagggt gacggcggag gtggagagtg
     2941 aggttgtcaa tcagcgtcgg ttgataggat atccaacaaa ataagtctga cgctttggag
     3001 tcgagtgcta ggttgatgag gtcaacgaaa gggaggtcga ggcgggatgt gcatttggaa
     3061 ggcttcgcgg gagcagttga gtttcgagtt cgggagtgct cgaggcggcg accggaaggg
     3121 cgcgcggcgc aagggccgta gagggcgtgg aaatggaggt gccgggcgcc ggaccgcgcc
     3181 tcacgtgaag aggccggcac ataggcgtta tgagccggtg catgtgacgg ttcgggtggg
     3241 ggtggggtgt ttgcgaaagc aggtggtgtt tgcggtgatt cggcgggcaa tcgcgcgggt
     3301 gaatggtatg cgggaggatc gatttcgagt ggtggagttt tcggtgcaag cgaatcacgt
     3361 tcacttcatc gtggaggcgg aggggcgaac ggagcttagc tgtgggatgc gtgctctgaa
     3421 tgcacgcatc gcgaagcagg tgaatcgtgt gctggggcgg cgggggcagt tgatgtcgga
     3481 acggcatcac gagcaacctc tgcgctgtcc acgcgcggtt cgtcatgccc tttgttatgt
     3541 gctggcgaac ttcaggaagc atgcgacgca cgaggggctg ggcggggaac cgctcgatat
     3601 ctgctcgtcg gctcgctact tcggcggttt tctggagttg aacagcacga cttcacgaga
     3661 gacgagcgga ccgaggcgcg cgcggggcgc gccggtggcg agcgcacgaa cctggttgct
     3721 atcggtcgga tggaagaagc acgggcttat atccattcac gaggggccgc ggggggccgc
     3781 ggtttagcga attgaatcca ttggcgtagg gtctccgtct acgcggcatg cgaagaccag
     3841 aagtcgatgt cgatccttcg tgaagaggaa cggcacgacc cagtgctgcg tccccaagag
     3901 tagtaacgca tttgacctca ggcttgaagg acgcggccgg agtacgtacg cgcgggcgat
     3961 gcgggtcacc gtccacgtcg catagtactc cgctggtcgc gtccggcgga gtccacacaa
     4021 agcaccgtcg tgcccacagg aggcgagatg tacagtttgc agatcttggc ggcttttgcg
     4081 tcgaaatctg ggtcgttcgg attgagccat taacgaactc gatgtggttc gagggccatt
     4141 gagcgcagga atcggcgaat cgaagagcag cccgacgtcc catccgaatc ggcgcgccag
     4201 gaactccgcc agagcccgca ccgaccgacg agcgagtgcc cagccgagct ttccggtggt
     4261 tggaccacga tcgtagcgag agcgtgggcg gtccttcagg tgccatcttg gcccatcgcg
     4321 ctgaatggtc tgcgtcccca aggttatctt cgttcgaacg aaacagcatg cttcggggca
     4381 actcgcgtgt atgatcggca cccatgacta gcaaacgcac ggcgatcgtt ctcggcggtt
     4441 ctggctcggt cggaatggcg ctgctgcgcg agttgtttca ggacgatggc ttcgaggtcg
     4501 tcatcacgtt gtcgcgcagg acgttgccgg aagccgtcgc gttggctcgc gcgagcgggc
     4561 gcaggttgct ggagaagctc gtgcccgaga tgacaccggc gctgctggcg gcagcgacgc
     4621 tcgaggccgc gaaggatgtc gaaggtgacc tagaaggttt cagcgtgctc ggggtcggtg
     4681 cgggcaccgc aaagctgacg ctcgatgaac atcgagcggt ggacgtggca ttgaacgagg
     4741 cgtttgcgcg cgccttacgc gattccggca aagtccggca tttcgcgttc atgacggcgg
     4801 tgggggcgga tccaaatgcc aaggcgagcg gcggcggagc cgcggggatg tcgcgttaca
     4861 accgcgtgaa gggcgaggcc gaggaagccg tgcgtggcgc gggcccttcg atcgtgagtg
     4921 tgttccgtcc ggcgatgatc atcggctctc agcacacgcc ctggctgctc gagaaggcgt
     4981 taccgttgtt ctcgttcgtc acgcccggta agttcaagtc aatcaccgtg gcgcagatcg
     5041 cgaaagcaat ggttgccacg gcgaagcacc accccgccgc gagcgcgacg tatcactacc
     5101 cggaaatgat ggctctgatc acctcatgtt gatcttcgag ggtgtctacg ccaccgcgca
     5161 ctcgtccaac gcccccgaga ccgcagactc aaatctccta ttgacccgca acaccgcgcg
     5221 ggccccgcaa taacggcgtt ccccttcgtc gcgttactgg gagcggcctg cgcgcggata
     5281 aagcggggtt cagctcgtat acgggccgta gcccgaaagg taacaaagcc cacccgcttc
     5341 gccgagcgct tccaattggt gaatggcttg cgtcgtgagg tcgatcgaga gccgggccgc
     5401 ggcgctgtga tgatagacgg cgctgacggt ggcccagtgg cgcgcatgat gcagcagata
     5461 gagagtttcc cgagccactc cgtggatgcc atatgcggcg gaggcgttcg tgacatacgg
     5521 tgagtcgagc ccattctcca cgagccaagt ctgaagtccc aggatctgac caagagcgtc
     5581 attgagccca tcgagattcg ttttcgcagc cgcggcatac acaccgccct cacaaaagct
     5641 ggcgcagtcc gcgttggctt gggccacact gtcctccagc gtcgcgatga gcgtcgagat
     5701 cgcggcgggg gtcagcggcg agcaactctc ggtcatctca tctgcccgcg caaattttgg
     5761 tgagaaggcc gcgcccgtga ccacgagggc gaacagcgcc tgtttcagaa ttagtttcat
     5821 ctgattcgtc ctttccgttc ggagatgccg ccgccccggc gaagtcgcac gaacgaaaca
     5881 aaatgcctac cgcggaaagc attccggcta cgacccgacc aagaatcatt ggtcactgca
     5941 gtttcgcccg ttggttgcgg ccgctctgtt actgaccttc caagcgagaa aaagatcgga
     6001 cggggatcga caagcacgag gaggtatgat cttttcgcat cgtgaggcct gtaggtacaa
     6061 agagcgagct tgagt
//
LOCUS       DASTKU010000926        15821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_402738, whole genome shotgun sequence.
ACCESSION   DASTKU010000926 DASTKU010000000
VERSION     DASTKU010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 15821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15821
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402738"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFQ61_32685"
     CDS             <1..216
                     /locus_tag="VFQ61_32685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959308.1"
                     /translation="GARSRARARRAYPGLTSPTVVSEQFTVREDPQARPEVSAGVLVL
                     DAFTQDRVSLIAARGVRLGGSLSGPGA"
     gene            386..2749
                     /locus_tag="VFQ61_32690"
     CDS             386..2749
                     /locus_tag="VFQ61_32690"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HET9959309.1"
                     /translation="MPDYLDQGTRYLLESPTLAPQASAYLWNRRMMLQITCRGYAVAQ
                     FMQPEPAKYAHVPTLAQTSFMQPEQPFFAHHPGRFFYVRDDETMDLFSAPYEPARVPL
                     DVFEFEPGLSDIRFRARKAGLELRLTLSLTKDDVVELWTAELTNCGHETRRVSLIPYF
                     PVGYASWMNFGGRFEPALGAVVASAVAPYQKLQDYFKRKDWKDLTYLVCDHDPIAYEA
                     NQAAFEGEGGLREPDALKRPMLQGGEAHYELPACALQFSFELPPGQTRTLKFLFGPAK
                     DHQEIRQLKQKYLTDGAFERAHEAYSEYVSAGAGCLRVQTPDPTFNSFVNHWLPRQVF
                     YHGDTNRLTTDPQTRNFMQDAMGMAFIDPRVTRRALLLALSQQKAKGEMPDGVLLYPG
                     AELKYINQIPHTDHAVWLVICLEAYLAETADWTLLDESLAFADDPAPASVYEHLCRAM
                     EWLISARDARGLSYIAQGDWCDPMNMVGYKGRGVSGWLTQALSFALQRFIPYCRARGD
                     ETRVRRYELTIQELNAAIQRELWDGSWYARGITDDGVSFGTRKDREGRIFLNTQSWAL
                     LCRAPTPDQIETLLQAVHDQLDTPYGVELCGPPFTHMREDIGRVTQKFPGSAENGAVY
                     NHAAAFYAAALYSIGKGDLGYSVLRRMLPGPELSDIQQRNQLPIFVPNYYRGAFRQLP
                     RTAGRSSQLFNTGTAAWFYRIVVERLIGLRGEGDGLHIEPLLPEAWPELRATRLFRGA
                     VLELRVTRGELSAQLIVVDGKVLTQPFLTPIRAGKKYTIEVQLPARS"
     gene            3405..5072
                     /locus_tag="VFQ61_32695"
     CDS             3405..5072
                     /locus_tag="VFQ61_32695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9959310.1"
                     /translation="MTKQEVLYAGRVLGRYELLMPVAKGGMAEVWAARQFGTRGGSKV
                     VAVKTILRTALDDARLERMFRAEASVASRIHHPNVVEMLEFDEYDGVLFLAMEWVDGE
                     PLNVVLAQANTQGGIPLDIGVNIIGQACLGLHAAHEARDEEGALLGVVHRDVSPQNVL
                     VSDAGAVKLLDFGIAKATARASALTAAGEIKGKFAYMSPEQVSGRELDRRSDVFSLGV
                     LLFMLTTGRHPFRGEHPGETVRRLYSDDPAVPPSRFMSNYPPALEDVLMRALAKPVDA
                     RWPTAHELRSALEAALPSAFEGQAEVRLSRYLHGLMGAQLAARRGQLRVAEESLADRR
                     QEALEPVPGSHGSLRAICIEPRRPVDSSAPVSQELVPRSSRSAQAVELRLMPVPAARS
                     WCQTACVTSGTLLLGIALTLGYQKVASSSAPSSIWREQAASSGDIPLTSALPASKPAG
                     HESCSFPQKGTVAEAGLTTASAGGSPAVISVSPVVAPTESASSPIVAPASSAAASVAR
                     LSPAPVSSVPTRRAAPPATPGGSPHEPKAKKLPEFRRTSNCVGGTCK"
     gene            complement(5043..6179)
                     /gene="hflX"
                     /locus_tag="VFQ61_32700"
     CDS             complement(5043..6179)
                     /gene="hflX"
                     /locus_tag="VFQ61_32700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET9959311.1"
                     /translation="MMEWSNRTSRAVLVCVARTPGSELTSSSQELVSLAKVLNIEVSS
                     VLVQRRSGHAGPRFLGEGKLSELARKVQGQDPAERSADCVLVDALLDPAQQRVLEQAT
                     ELPVYDRTAVVLQVFARHARTRQAQLQVELARLQYQLPRLRDDSSKAGREGGGGRGER
                     GHKNLVLEKQRCRARIAEVRQELARAERNFSIQRERRDGIPEVALIGYTNVGKSSLLR
                     ALTGSDVLIRDEPFATLGTTVRRLSRTGGRTILVSDTVGFMQGLPHELVAPFHATLEA
                     ARHAKLLLIVADASSAICADQLRVTEETLVQIGAQEVPRTYVFNKMDRLSPAQRESLQ
                     QIYPEALYISAHSADDVERLRAHVQASVIEPAESGLLAGAANAV"
     gene            7092..9179
                     /locus_tag="VFQ61_32705"
     CDS             7092..9179
                     /locus_tag="VFQ61_32705"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET9959312.1"
                     /translation="MLFGVLLGVLLSACGSSSSSEPPPAPTQCSEATARVGGLACLHR
                     LTSDAEWQSISVGLPTADRVRAAKYLVPAAPQAALDTLFMNSKRFALHYDFLRQVFPD
                     LFPGLSPNEYLALLFDPNRRQYYVGDLAEYHLPTGEVRFGFTLVTDPKSPEAVSCDDL
                     VRVHAALASRLYGEDLYVVPYDKYQLGFAPRCGLPLLDPSQTLSYEVYHEGVGFGTLR
                     RYSAGALKDAITNADFGFRDLLVLEEAPTDVETVVSGVVTSSRQTPLSHLAVRSASRG
                     TPNCFQAGAFEALAPWEDQLVRIECGAGELLVRGAELEEAEAFWSALRPTPVELPDPD
                     LEFMELPALEQVPTDGPAQRALALRRFGAKAMNLAWLRQNMARELTPMGFVIPVAHYA
                     EFMENTTWQTDLGSGSAAHSFADTLAAWLADPIFRTDAKVRKARLLQLQDAMVAAPCD
                     AELQTSIAESIRALVGSESTTLRFRSSSNAEDGVTFNGAGLYDSYSGCLADDLDADDA
                     GPSICDPEERKERSVCRALKRVWASLWNPRAYDERDFYGIEQARVMMGVLVNERSEKE
                     RANMVVFNGNPARSGDARYLVNAQIGELAVVSPEPGVWPEQVLLTLRAGEVSNIERIA
                     ASSETAVGLPVLSDAELRALGAHMAQLSGLYPFDVSAPAGHTLLLDTEWKVMPDGSLR
                     IKQVRPFSRRDRN"
     gene            complement(9139..12972)
                     /gene="metH"
                     /locus_tag="VFQ61_32710"
     CDS             complement(9139..12972)
                     /gene="metH"
                     /locus_tag="VFQ61_32710"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET9959313.1"
                     /translation="MAELHPLQKLLQNRIAIIDGAMGTTIRTYGMKEADIRGQRFADA
                     SKDLLNNGDLFSLTQPTMICDIHRRFLEAGADIIETNTFGATSITQSEFFIDDPREHG
                     GRKDPEFYQKVLENPFLRQLAYDINVTSARQCREWADRVANASSRPRFVAGAIGPLTV
                     SLSNSPDAEDPGFRVCTFDQVKLAYVEQIRALIEGGSDVLLVETIFDSLNAKAALVAI
                     EEVYQSLGKRLPVMVSAAVGRGGETMISAQTVEALWNAVEHIRPLSIGLNCSLGPDVM
                     YPFLSELAAKADTAISCYPNAGLPNPLSPTGFDLEPPDMARFLTQFAESNLVNIAGGC
                     CGNTPEHIAAIARALEGRHPREIAERSRTTVPVSNALADVRPLRLSGSQPFTQQAGTF
                     LMIGERTNVAGSPKFAKLIKEGKYDEAVGIARQQVENGANVIDICMDEGMIDGVQAMT
                     RFLLLLGSEPEVAKVPFMVDSSKWEVIEAGLKCLQGKGIVNSISLKEGEARFRECAQT
                     VLKYGAAVVVMAFDEQGQAATYQDKIRICERAYRILVDEVGFPPEDIIFDPNILTVAT
                     GMEEHNNYAVDFIEATRWIKTHLPHAKVSGGVSNISFSFRGNNPVREAMHAAFLYHAI
                     RAGMDMGIVNAGMLEVYEEIRPELKELVEDVLLNRRADATERLVEFGEKLKSAASSPT
                     GTAEKQTEEWRKASVEERLSHALVKGIDTYIDQDTEEARVKLGRPLLVIEGPLMDGMS
                     VVGDLFGAGKMFLPQVVKSARVMKKAVAYLTPFMEAEKAEMARAGQVVKTQGKIVLAT
                     VKGDVHDIGKNIVGVVLACNNYEVFDLGVMVPCEKILERARQENADLIGLSGLITPSL
                     DEMTHVAREMERQGFKLPLLIGGATTSRAHTAIKIAPHYSQPVVHVLDASRAVPVTTS
                     LLSKENRAAFIEQHQAEYERLRRTHAGPKLRLTSIDRARDNRTPIEWKREDLAQPEFL
                     GVRVIEDMSLGTLAEFIDWTPFFHTWELKGVYPRILEHEKYGEQARQLFAEGQALLAK
                     LVRDKSLRARGVYGFFPANAVGDDVELYADETRTRVVERFHFLRQQLLKEAEPNRSLA
                     DFVAPKSTGLPDYIGAFAVTTGHGLRELCEQLRARHDDYNAIMAEALADRLAEAFAEY
                     LHQKVRREWGYGRDERLSQAELIEEKYRGIRPAAGYPACPDHTEKGTLWRLLSVESHT
                     GMQLTESYAMWPGSSVSGLYFGHPSSQYFSLGKIDRDQVLDYHVRKGMTVGEVERWLA
                     PNLNYDPAEKTGGPA"
     gene            complement(12972..13898)
                     /locus_tag="VFQ61_32715"
     CDS             complement(12972..13898)
                     /locus_tag="VFQ61_32715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9959314.1"
                     /translation="MRLSDYVETLNLFCDESRLRLCALLRDRELCVTDLVQVTGLAQS
                     RVSTHLGRLRDAGFVRDRRAGAQAFYSLSEETLPSPARAMLEEALGSSDPTLEGDRRR
                     LRELETERRGGASPGFADEIERFYSPGRSWQSLAAGLAGLLRLGNVLDVGSGDGAAAS
                     AIAPYCKALTCIDVSPRAVESARVRLAPWAHVQVRTGDAHELPFAAATFDQVLLFHTL
                     TYTERPAQALSECARVLKAGGRLVLLCLDRHMQQDVTAPYGERHAGFSPRSVRRLLSG
                     AGLHVVNSDVACRESKKPHLQVVLAIAEKPES"
     gene            14286..15026
                     /locus_tag="VFQ61_32720"
     CDS             14286..15026
                     /locus_tag="VFQ61_32720"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9959315.1"
                     /translation="MKAVILGGTSGMGRAIAQQLAERGETVFILGIGREDLERSAADL
                     TLRSPTRATVGYAELDLEKPEGFAPALDAADAALGGFDTVIVTAALFASQEALEADIE
                     LTRRLVTVNFAHTVVFCEHVRKRLLARGGGQLTVFSSVAGDRGRKPVAIYGASKGGLS
                     VYLEALDHKFHAAGLSVLCVKPGFVKTAMTAGLKPPPFAGEPEQVAKQVIRAMDQRKA
                     LIYAPAIWRLILLVIRFLPRFVMRKINF"
     gene            complement(15050..>15821)
                     /locus_tag="VFQ61_32725"
     CDS             complement(15050..>15821)
                     /locus_tag="VFQ61_32725"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9959316.1"
                     /translation="RSSFGAMTERVLILGATSAIALEAAKLYALRGARLHLVARNADK
                     LQGVLARLRGSSEAEVTQETADFSDLSANAELVSRAERSLGALDTVLIAHGDLGNQLE
                     TERSFDAAEHVLRVNFMSVVSLLIPLSQRMEAARHGRICVITSVAGERGRPRNYTYGT
                     AKAALNVYLQGLRSRLYGTGVTITTIKLGPVDTPMTVSHKKSILFAQPESVARSIVAA
                     TDLRRAEVYVPSYWRPIMSIVRNTPERLFQMLPFLSGR"
BASE COUNT         2943 a         5068 c         4809 g         3001 t
ORIGIN      
        1 ggtgcgagaa gccgagctcg cgcgcgtcgc gcgtatccgg ggctgacttc acccaccgtg
       61 gtgagcgagc agttcacggt gcgtgaagat cctcaggcgc gacccgaggt gagcgcgggt
      121 gtgctcgtgc tcgatgcgtt cacgcaagat cgcgtgagcc tcatcgctgc gcgcggagtt
      181 cgacttggtg gatccctatc agggcctgga gcttaagctg cgaagcatcc aggagtcgct
      241 cgagttagtc ttggatgcag cgcgtgatcg ccggttggtg ctgctcgagc caaggggcag
      301 tcgagctgcc gttcgtctat cacgtattcg acgctgctcg aggatggact tccgcccgga
      361 tcgcctttaa ctaacccgcg ttacgatgcc cgactatctc gaccagggaa cccgctatct
      421 gctcgaaagc ccgacgttgg cgccgcaggc gtcggcgtat ctatggaacc gacgcatgat
      481 gctgcagatc acgtgtcgcg gttacgccgt cgcgcagttc atgcaaccgg aacccgcgaa
      541 gtacgcccac gtgcccacgc tggcccagac cagcttcatg cagcccgagc agcccttctt
      601 cgcccaccat ccggggcgat ttttctacgt gcgggacgac gagaccatgg atctcttctc
      661 cgcgccctat gagccggcac gcgtgccgct ggacgttttc gagttcgaac cgggcctaag
      721 tgatattcgc tttcgtgctc gcaaagccgg gctcgagctg cgcctgacgt tatccctgac
      781 caaagatgac gtggtggagc tctggaccgc agagctcacc aattgtggcc acgagacgcg
      841 tcgggtttcg ctgatcccct actttccggt cggctatgcc tcgtggatga acttcggagg
      901 gcgcttcgaa cccgccctcg gggccgtggt cgcctccgcg gtggcgccgt accagaagct
      961 gcaagactac ttcaagcgga aggattggaa ggatctcacg tacctcgtat gcgaccacga
     1021 tccgatcgcc tacgaggcca atcaagccgc cttcgagggc gaaggcggcc tgagagagcc
     1081 ggatgcgttg aagcgcccaa tgcttcaagg cggcgaggcg cactatgaac tgccggcgtg
     1141 tgccttgcag ttctctttcg agctcccgcc gggccaaact cgcaccttga agttcctgtt
     1201 cggtcccgcc aaggatcatc aggagatccg ccaactcaaa caaaaatacc tgaccgacgg
     1261 agcgttcgaa cgagctcacg aagcatactc cgagtacgtg tccgctggcg ccggctgcct
     1321 ccgtgtgcag accccggatc ccaccttcaa cagtttcgtc aatcattggc tcccgcgcca
     1381 ggtgttctat cacggtgaca cgaatcgact gaccaccgat ccgcaaactc ggaacttcat
     1441 gcaggacgcg atgggcatgg ccttcatcga cccacgcgtc actcgccgcg cgctcctgct
     1501 ggccctctcg cagcaaaagg ccaagggcga aatgccggat ggggtgctgc tctatccggg
     1561 tgccgaactc aagtacatca accaaatccc acacacggat catgcggttt ggctggtgat
     1621 ctgcctcgaa gcctatctcg ccgaaaccgc cgattggacg ctgttggacg agtctttggc
     1681 cttcgccgac gatcccgcgc ccgccagcgt ttacgagcac ctgtgccgag ccatggagtg
     1741 gctgatctcg gcccgcgacg cgcgcggtct ctcctatatc gcgcaaggcg attggtgcga
     1801 tccgatgaac atggtcggct acaagggtcg cggtgtctcg ggttggctga cccaagccct
     1861 ctccttcgct ctgcaacggt tcatccccta ctgccgggcg cgaggcgacg aaaccagggt
     1921 gcgtcgctac gaacttacca tccaggagct caatgcggcc attcagcgcg agctgtggga
     1981 cggctcgtgg tacgcgagag ggatcaccga cgatggtgtg agctttggaa ctcgcaaaga
     2041 tcgcgagggg cgcatttttc tcaataccca gagctgggca ctcctgtgcc gcgcgccgac
     2101 tcccgaccaa atcgagactt tgctgcaagc cgtgcacgac caactcgata cgccgtatgg
     2161 ggtcgagctg tgtggaccac cattcaccca catgcgtgaa gacatcgggc gcgtgacgca
     2221 gaagttcccc ggctctgcgg agaacggcgc cgtatacaac cacgccgccg ccttttacgc
     2281 cgctgccttg tactccatag gtaaaggtga cctcgggtac tccgtgcttc ggcgcatgct
     2341 gccgggcccc gagctcagcg acattcagca gcgcaatcaa ctacccattt tcgtaccgaa
     2401 ctactatcgc ggtgccttcc ggcagctgcc tcgcacggca ggccgctcga gccaactctt
     2461 caacaccggc actgccgcct ggttctatcg gatcgtagtg gaacgattga ttgggttgcg
     2521 cggagagggg gatggtctgc acatcgaacc gctacttccg gaagcgtggc ccgagctccg
     2581 cgcaacgcgc ttgtttcgcg gtgccgtcct ggagctgcgc gtcacacgcg gcgagctctc
     2641 cgcgcaactc atcgtcgtcg atggaaaagt gctcactcag ccttttctga cgcccatccg
     2701 agcgggcaag aagtacacga tcgaggtaca gcttcccgcg cgatcgtaag ccatagtcct
     2761 gactccgacg gggggtgaag aagttgtgga aagaatcgcg acctacgcgc gcgaagattt
     2821 acggcttctt tgccgcgtgc ccgacgtgga tcgcccgtct cgaagcaaga aacggcggcc
     2881 ctctgtgagc tcgtgtgcgt gaattctccc ggtggatgtg ttgttaccaa gggtgttacc
     2941 gccgcgaggg cgcttccgag ccgcgaattc ccgaaacgtc agcggtgact tggagtgctg
     3001 gggaggacac ttcgagagca cgccgagagt gtcgaaacgc atgcacgagt ggctcgcgca
     3061 gacctagaca ctcgtcttcc gtgttcgctc gacacgaatc gttgcgtacg aatgaacgcc
     3121 gtaccgacgg agatttcggc tgaaatcagc gcacgaacgc gcggctggag tgcgcccaat
     3181 caggtctcgt gctcagtgac caaggcggct ggcgtgttcc gtgaactcga cctgactcga
     3241 acacatgacg ttggagcatg cgtcgtgcac tcgtgagcga cgacgcttca ccatttgcat
     3301 gctcgacatg ctagcttgct cgagcgagtc ggttgcaaac cggggcgctg cgcaccgcgg
     3361 ctcggatttg cctcaatttg atcaactggc acggagggtt gcccatgact aagcaagagg
     3421 ttctttacgc aggccgtgtc ctgggtcgtt acgagctctt gatgccggtc gccaaaggcg
     3481 gcatggccga agtctgggcg gctcgccagt tcggcactcg gggcggaagc aaagtggtgg
     3541 cagtgaagac catcctgcgc acggccctcg acgacgcacg gctcgaacgc atgttcagag
     3601 cggaggcctc ggtagcctcg cgcatccatc acccgaacgt cgtcgagatg ctggagttcg
     3661 acgagtacga tggggtcttg ttcctggcca tggaatgggt ggacggcgaa cctctgaatg
     3721 tggtgctggc gcaggccaac actcaaggag gcattccact cgacatcgga gtcaacatca
     3781 ttgggcaggc gtgcctcggc ttgcacgcgg cgcacgaagc ccgcgacgag gagggagcct
     3841 tgctcggcgt agtccaccgt gacgtctcac ctcaaaacgt cctggttagc gatgcggggg
     3901 cggtcaaact cctggatttc gggatcgcca aagccacggc gcgcgcctcc gcgcttaccg
     3961 ccgctgggga gatcaaaggc aagttcgcgt acatgtcccc cgagcaggtg agcggacgtg
     4021 agctcgatcg gcgctccgac gtgttttcat tgggtgtgct gttattcatg ctcacgacgg
     4081 gtcgccaccc ctttcgaggt gagcacccgg gggagacggt gcgccgtcta tattcggacg
     4141 atcctgcagt gcccccgagt cgcttcatgt ccaattaccc tccggcactc gaagatgttt
     4201 tgatgcgcgc gctcgccaaa cccgtggacg cgcgttggcc gaccgcgcac gaactgcggt
     4261 ccgcgttgga ggcggcgctg ccgtccgcgt tcgagggaca agccgaggtg agactgtcac
     4321 gctatcttca tggcctgatg ggcgcgcaac tcgcagcgcg ccgcggtcag ctgcgcgtgg
     4381 cggaggagtc gctcgcggac cgacgccagg aagcgctcga accggtcccc ggaagtcacg
     4441 ggtctttacg agcgatttgc atagagccgc ggcgcccggt cgacagctcc gcacccgtta
     4501 gccaggagct cgtaccccgc tcctctagaa gcgcgcaagc cgtggagctc cggctcatgc
     4561 cggtacccgc cgcgcggtca tggtgtcaga cggcctgcgt caccagtggg actctgttgc
     4621 tcggcattgc gctcacattg ggctatcaaa aggtggcctc atcgagtgct ccgagctcca
     4681 tatggcgcga acaggcggct tcgtccgggg acatcccgct cacgagcgcg ctccccgcga
     4741 gcaaacccgc tggccacgaa tcatgctctt ttccgcaaaa gggcaccgtg gccgaggccg
     4801 gactcaccac cgcctccgcc ggtggctcgc ctgccgtcat ttccgtttcc cccgtcgtag
     4861 ccccgaccga aagtgccagc tcgccaatcg tagccccggc atcgtccgct gccgcgagcg
     4921 ttgctcgcct ctcccctgct ccagtttcct cggtgccgac ccgaagagcg gccccgccag
     4981 caactcctgg cgggtcgccc catgagccca aagcaaagaa gcttcctgaa tttcgacgaa
     5041 cgtcaaactg cgttggcggc acctgcaagt aagccgcttt ccgcgggttc gatcacgctg
     5101 gcctgaacgt gagcgcgcaa ccgctccacg tcgtcagcgc tgtgcgcgct gatgtagagc
     5161 gcctccgggt agatctgctg caggctctct cgttgcgctg gcgacaggcg gtccatcttg
     5221 ttgaacacat acgtgcgggg cacttcctgc gcgccgatct ggaccaacgt ctcctcggtc
     5281 acgcgcagtt gatctgcgca aatggcgctg gacgcgtctg ccacgatgag caagagcttc
     5341 gcatgtcggg ccgcctccaa cgttgcgtga aaaggcgcga ccagctcgtg cggtagaccc
     5401 tgcatgaacc ccaccgtatc ggacaccaga atagtgcgac cgccggtccg tgacaggcga
     5461 cgcacggtcg tgcccaaggt ggcgaagggc tcgtcacgaa tcaggacatc actgccggtg
     5521 agggctcgca ataagctcga cttcccgacg ttcgtgtagc caatcagggc cacttccggg
     5581 atgccgtcgc gccgctcgcg ctggatcgaa aagttgcgct ccgcgcgggc gagctcttgc
     5641 ctgacttccg cgattcgtgc gcggcacctc tgtttctcca aaaccaggtt cttgtgtcca
     5701 cgctccccgc ggcctcctcc gccttcgcgc ccggccttgg aggagtcgtc ccgcagcctc
     5761 ggcaactgat actgcaagcg cgcgagctcg acttgcagct gagcctgtct cgtacgagcg
     5821 tgtcgcgcga acacctgtag aacgaccgcc gtgcgatcat agacaggcag ctccgtggcc
     5881 tgttccaaaa cacgctgttg agcgggatcc agcagagcgt ctaccagcac gcagtccgcg
     5941 cttcgctccg cgggatcctg accctgaact ttccgggcca actccgataa cttgccttcc
     6001 cccagaaagc gcggcccggc gtgccccgag cgccgctgca ccagcacact cgacacttcg
     6061 atattcagca ctttcgccaa actcacgagc tcctgactcg agctcgtcag ttccgagccc
     6121 ggcgtcctcg cgacacaaac caacaccgcg cgcgaggtac gattagacca ttccatcatt
     6181 ttgtaagtct ttctggacag attcctcgga acccggccct gcattggcgc ctactgcagg
     6241 cgcacttccc cgttcagaac tcgatatttc aagttctcga ttcgaccaaa catcggattc
     6301 agtgaaaaca cgccgagtcc ctgcgcgcgc gtcgacacca gtgcttccca gacccagcga
     6361 gcgagcccat cttcgatggt cgcgtactgc gggcgacgcc gctggacttt cgtcagggtc
     6421 agcgcctacg cgggcgagtc gcggctcgga gaaacccggc gccggatcgt gccaaacgag
     6481 tttcctcgct ggctcgaatg gcctcggcgt gtcgatcagc tacgtagact tctggcactt
     6541 ccgtgcggac cgccgcggac gtggctcgca tgaaacgaac caggttacct cggcaaccac
     6601 aatagaatcc gaaactacgt accggttacg gacgggagca ctccacgtca gcgaaaccga
     6661 atgagcaaca tgcgcatagc ggtcggaatc taactcaaac tcgaccgatg gcaagcgggt
     6721 acgccgcaat tactcggcga acatccaacc ctcgcacttc cggtccgagc cgctgctgcg
     6781 actacacgga gctaggcgtg cgcgacttgg agcttcactc ctcctcggac ccgccccagt
     6841 cttctggacg acaatcgact ccgggatggc gcgagctggc acacatgccg actgcataca
     6901 ctcgcgggag cgggtcgcgc gcgagttgct aggaatatgt aagcgtttga gaacgctcga
     6961 agtagccagg ccgggccgtc cgtggcacgt ggcatgcagc gacatgtcgc ggagtgttga
     7021 ccttggttat tgatgggaag acccgtcgcg cggcagcgcg gctctgtacc tccgcactgt
     7081 tgtttctcgg catgcttttc ggcgtgcttc tcggcgtgct cctcagcgcc tgcggcagtt
     7141 cgagctcgag tgagccgccc cctgcgccga ctcagtgctc ggaagccacg gctcgggttg
     7201 gcgggctggc ttgcctacat cgtttgacct cggacgcaga gtggcaatcc atctccgttg
     7261 ggttgccgac ggcggatcga gttcgcgcgg ccaagtatct cgtaccggcg gctccgcagg
     7321 ctgccctcga tacgcttttc atgaactcga agcgctttgc gcttcactac gacttcctac
     7381 ggcaagtctt tccagaccta tttccagggc tctcaccgaa cgagtatctg gccctgctct
     7441 tcgatccgaa tcgccggcag tactacgtcg gagatttggc agagtaccac cttccaacgg
     7501 gcgaggttcg cttcggcttc acgctcgtca ccgatccgaa atccccggag gcggtcagct
     7561 gtgacgactt ggttcgcgtc cacgcggccc tcgcatcgcg actttacggg gaagacctgt
     7621 atgtggtgcc ctatgacaag tatcaactcg gcttcgcacc acgctgcggg ctcccgctgc
     7681 tcgatccgtc ccagactctg tcttatgagg tctatcacga aggagtcgga ttcggcacgc
     7741 tgcggcgcta ctcggccgga gctctaaaag acgcgatcac caacgccgat ttcgggttcc
     7801 gcgacctgct cgtactcgag gaggctccca ctgacgtaga gacggtcgtc tccggtgtgg
     7861 tgacgagcag ccgacaaact ccgctctccc atctggccgt gcgcagcgcc tcgcgcggaa
     7921 ctccgaactg ctttcaggcc ggtgcgttcg aggcgctcgc gccatgggag gatcagctgg
     7981 tacgaatcga gtgtggtgcg ggtgagctcc tggtcagagg ggctgaactg gaagaggccg
     8041 aagctttctg gagcgctctg aggccaacgc cggtcgaact accggatccg gatctggaat
     8101 tcatggagct ccccgcactt gagcaggtcc cgacagatgg ccccgcgcaa cgcgcgctgg
     8161 cgctgcggcg tttcggggca aaagccatga atctagcctg gctccgccaa aacatggcgc
     8221 gcgaactcac gccgatgggg ttcgtgatcc cggttgctca ctacgccgaa ttcatggaga
     8281 acaccacctg gcaaaccgat ctgggctcgg gctccgcagc acattccttc gctgataccc
     8341 tggcggcttg gctcgccgat ccgatattca ggaccgacgc gaaggtgcgg aaggcgcgct
     8401 tgctccagtt gcaagacgcg atggtggcag cgccctgcga tgccgaactg caaacctcga
     8461 ttgccgaaag cattcgcgca ctcgtcggta gtgaaagcac gacgctgcgc tttcgcagca
     8521 gcagtaacgc ggaggacgga gtcacgttca acggagcagg tttgtacgac tcttacagcg
     8581 ggtgcctggc cgatgacttg gatgcagacg atgcaggccc gagcatttgt gatcccgagg
     8641 aacgcaaaga gcgcagtgtg tgtcgcgcgc tcaaacgtgt ctgggcttcc ctctggaatc
     8701 cacgggctta cgacgaaagg gacttctacg gcatcgagca agcgcgtgtg atgatgggcg
     8761 tactggtgaa cgaaagaagt gagaaagagc gcgccaacat ggtcgtgttc aacggcaatc
     8821 cggcgcgtag cggcgacgcc cgctacctcg tcaatgccca gatcggagag ctcgccgttg
     8881 tgtcccccga gcccggcgtg tggccggagc aagttttgct cacgcttcga gcgggggagg
     8941 tctccaacat cgagcgaatc gccgcctcga gcgagaccgc cgtagggctc ccggtgctga
     9001 gcgacgccga gctgcgcgcc ctcggtgcac acatggcgca gctctccggg ctctatccct
     9061 tcgatgtgag cgctcccgcc gggcacaccc ttctcttgga cacggagtgg aaggtgatgc
     9121 ccgacggatc gctgcgcatc aagcaggtcc gcccgttttc tcggcgggat cgtaattgag
     9181 gttgggggcg agccagcgct cgacttctcc aaccgtcatg cctttgcgta cgtggtaatc
     9241 gagcacctgg tcgcgatcga tcttgcccaa gctgaagtat tgcgagcttg gatgcccgaa
     9301 atagagacca ctcacgctcg agccaggcca catggcgtaa gactcggtga gctgcatgcc
     9361 cgtgtgactc tccacggaaa gcaagcgcca gagcgtgccc ttctccgtat gatcggggca
     9421 ggccgggtag cccgcggccg gacgaatgcc gcggtacttc tcctcgatga gctcggcttg
     9481 gctgaggcgc tcatcgcgcc cgtagcccca ttcgcgccgc accttctggt gcaggtactc
     9541 ggcgaaagcc tccgccagtc gatcggcgag agcttccgcc atgatggcgt tgtagtcgtc
     9601 atggcgggcg cgcagctgct cacagagctc ccgtagtccg tgaccggtcg tgaccgcgaa
     9661 agctccgatg tagtcgggta gaccggtgct tttgggggct acgaagtcgg cgagtgagcg
     9721 gttcggctct gcctctttca gcagttgctg gcgtaggaag tgaaagcgct cgacgacccg
     9781 agtacgagtt tcgtccgcat acagctccac gtcatccccc accgcgttgg cggggaagaa
     9841 gccatacacc ccccgagcgc gcaacgactt gtcccgcacc agcttggcga gcagcgcttg
     9901 gccttccgcg aacagttgtc gcgcctgctc tccgtatttc tcgtgctcca gaatgcgagg
     9961 atagacgccc ttcagctccc aggtatggaa gaacggcgtc caatcgatga actccgcaag
    10021 cgtgccgagt gacatgtctt caatcacacg cacgcccaag aactccggct gagccaggtc
    10081 ctcacgcttc cactcaatcg gagtacggtt atcgcgcgcc cgatcgatcg atgtgagtcg
    10141 aagctttggt cccgcatgcg tgcgccgcag gcgttcgtac tcggcttgat gttgctcgat
    10201 gaacgcggct cggttctcct tgctcaggag gctcgtcgtt acggggaccg cgcggctcgc
    10261 atctagtacg tgcaccaccg gctggctgta atggggcgcg atcttgatcg cggtgtgagc
    10321 gcgactggtg gtcgcgccgc cgatcaagag cggcagcttg aagccttgcc gttccatctc
    10381 tcgcgcgacg tgggtcatct cgtcgagcga tggcgtgatt aggccgctca ggccgattag
    10441 atcggcgttc tcctggcgcg cgcgctccag gatcttctca cagggcacca tcacgccgag
    10501 atcgaagact tcgtagttgt tgcacgcgag caccacgccc acgatgttct tgccgatgtc
    10561 gtgcacgtca cccttgaccg tcgcgagcac gattttgcct tgggtcttga cgacctggcc
    10621 ggcccgcgcc atctcggctt tctccgcttc catgaagggc gtgagataag ccaccgcctt
    10681 cttcatcacg cgggccgact tgacgacctg cggcagaaac atctttcccg cgccgaacag
    10741 atcacccacc acactcatac cgtccatgag cgggccttcg atgaccagga ggggccgtcc
    10801 gagcttgacg cgcgcctctt cggtatcctg atcgatatag gtatcgatgc ctttgaccag
    10861 cgcatgggaa agccgctctt cgacgctcgc cttgcgccac tcttccgttt gcttctccgc
    10921 ggtgccggtt gggctactgg ccgcgctctt cagcttctcg ccgaactcca ccagccgctc
    10981 tgtcgcgtcg gctcggcgat tgaggagcac gtcctccacc agttccttga gctcgggccg
    11041 gatctcttcg tacacctcga gcataccggc gttcacgatg cccatatcca tgccggcacg
    11101 aatcgcgtga taaagaaacg cggcgtgcat ggcttcccgc accgggttat tgccgcgaaa
    11161 gctaaagctg atattggaga cgccgccgct caccttggcg tgcggcaaat gggtcttgat
    11221 ccaacgcgtg gcctcgatga agtcaaccgc gtaattgtta tgctcctcca tgcccgtcgc
    11281 cacggtgagg atgttcggat cgaagatgat atcctccggc gggaacccga cctcgtcgac
    11341 caagattcga taggctcgct cgcaaatcct gatcttgtct tggtacgtcg cggcctgccc
    11401 ctgttcgtcg aacgccatca cgaccacggc cgcgccgtac ttgagcaccg tctgcgcaca
    11461 ttcgcgaaag cgcgcttctc cctctttcag agagatcgag ttgacgatac ctttgccctg
    11521 taaacacttc agccccgcct cgatcacctc ccatttcgag gagtcgacca tgaacggcac
    11581 cttggcgacc tccggctcgc tgccgagcag cagcaggaac cgtgtcatgg cctggacgcc
    11641 atcgatcata ccttcatcca tgcagatatc gatgacgttg gcgccgttct ccacttgctg
    11701 gcgcgcaatc ccgacggcct cgtcgtactt gccttccttg atcagcttgg cgaacttcgg
    11761 cgagccggcc acgttcgtgc gctcgccaat catcagaaag gtcccggcct gttgcgtgaa
    11821 cggctgcgag cccgacaagc gcaacgggcg cacatcagcc agagcattgc tcacgggaac
    11881 cgtcgttcgt gagcgctcgg cgatctcacg agggtgccgc ccttcgagcg cacgagcgat
    11941 tgcggcgatg tgctccggag tattgccgca gcaaccaccc gcgatgttga ccagattgct
    12001 ctccgcgaac tgagtcagga agcgcgccat atcgggcggc tcgaggtcga agcccgtggg
    12061 agacaatggg ttcgggagcc ccgcattcgg gtaacacgat atcgcggtat ccgccttggc
    12121 cgcgagctcg gacaagaagg gatacatcac gtcggggccg agcgaacaat tcagtccgat
    12181 ggacagcggt cgaatgtgct cgaccgcgtt ccacagcgct tccaccgttt gtgcagaaat
    12241 catggtctcc ccgccgcgac ccaccgccgc ggacaccatg accggcagac gcttgccgag
    12301 cgactgatag acctcctcga tcgcgacgag cgcggccttg gcgttgagcg aatcgaagat
    12361 ggtctcgacc agcaacacat ccgagccgcc ctcgatgagc gcgcgaattt gctcgacata
    12421 ggcgagcttg acttgatcga acgtgcagac ccgaaagccc ggatcctccg cgtccggaga
    12481 gttcgacagc gaaacggtca aaggcccgat ggcgcccgcc acgaagcgag gacgtgagct
    12541 cgcgttggca acccgatcgg cccattcgcg gcactgccgg gccgacgtaa cgttgatgtc
    12601 gtacgctagc tgacgcaaaa atggattctc gagaaccttc tgataaaact ccggatcttt
    12661 tcggccgccg tgctcccgcg gatcgtcgat gaagaactca ctctgcgtga tgctggtggc
    12721 cccgaaggta ttcgtctcga tgatgtccgc gccggcttcg aggaagcgcc ggtgaatgtc
    12781 gcagatcatc gtcggctgag tcagcgagaa gagatcgccg ttgttgagca gatctttgct
    12841 cgcatccgcg aagcgttgcc cccggatgtc ggcctccttc atgccgtacg tccgaatcgt
    12901 ggtgcccatg gccccatcga tgatggcaat gcgattttgg agcagcttct gtaacggatg
    12961 aagttcggcc attaggactc tggtttctcg gcgatggcta gcaccacctg aaggtgtggt
    13021 ttcttcgatt cgcgacaagc cacgtccgag ttcacgacgt gcaggcctgc gccagacaag
    13081 agacgacgaa cgctgcgcgg agagaagccg gcgtgacgct cgccgtacgg agcggtcacg
    13141 tcctgctgca tgtgccggtc gaggcacaat agcaccaagc gcccgcccgc tttgagcact
    13201 ctggcgcatt cggacagcgc ctgcgcgggc cgctccgtat aggtgagcgt atgaaacagc
    13261 aggacctgat cgaaggtcgc cgccgcgaac ggcagctcgt gcgcatctcc ggttcgaacc
    13321 tgcacgtgcg cccagggcgc caagcgtacg cgcgcacttt cgacggcgcg agggctaacg
    13381 tcgatacagg tcagcgcttt acaataggga gcaatcgcgc tcgccgccgc gccgtcacca
    13441 gaacccacgt ccaacacatt tccgagccgc aacaaccctg cgagccccgc ggcgagggac
    13501 tgccaggaac ggcccggaga gtagaagcgc tcgatctcgt cagcaaagcc cggcgacgca
    13561 ccgccccgac gttcggtctc gagttcgcgc agccgacgac gatccccctc cagcgtgggg
    13621 tccgacgaac cgagcgcctc ctccagcatg gctcgcgcgg ggctcggcaa ggtttcctcc
    13681 gaaagcgagt agaacgcctg cgctcccgcg cgacgatccc gcacgaatcc agcatccctc
    13741 aagcgaccca agtgggtcga aacgcgcgac tgggcgaggc cggtgacctg caccagatcc
    13801 gtgacgcaaa gctcccgatc ccgaagcaag gcgcacaggc gcagccgact ctcgtcgcaa
    13861 aacaagttca gcgtttctac gtagtcggac agtcgcatca ggcgttcggg tcgagcaggc
    13921 gcacggggtc actgtcgggg gtcgcgtctc caagcatcca agtatctaga tataaggatg
    13981 cccggctagg tattgctcgg gcctcggatt ccgtcaagtt cggcctagtg ccggcccgta
    14041 catcccggcg cgcccagagt gaccgcccac ccggccagtg cgccttgacg gcatgctagc
    14101 cagaccggaa acgctcacgt cgacaagtcg aatgagatcg tgttagcggc acctggtcgg
    14161 atcaccaacg aggatgcatt gatgcgcgcg gacccacgct cgatagaact ccggagcatg
    14221 cgagcccaac accggatgag ctcagcgcga aggggcgcgc accaccgcca gacaggagat
    14281 cgagcgtgaa ggccgtcatt ctgggcggga cgagtggtat ggggcgcgcc atcgcgcaac
    14341 aactcgcgga gcggggggag accgtattca tactcgggat cggacgtgag gatctcgaac
    14401 gcagcgccgc cgatctcacg ctgcgtagcc caacccgtgc cacggtcggt tacgccgagc
    14461 tggatctgga gaaaccagag ggcttcgcgc cggcactcga tgccgccgac gccgcgttgg
    14521 gcggcttcga taccgtgatc gtgacggccg cgctgttcgc gagccaggaa gcgctcgagg
    14581 cagatatcga gctgactcgg cgtctggtga ccgtaaactt cgcccatacc gtggtgttct
    14641 gtgaacacgt gcggaagcgc ttgctggccc gcggcggggg acaattgacg gtgttctcat
    14701 ccgttgcggg agaccgcggc cgcaagccgg tcgccattta cggcgcgagc aagggcgggc
    14761 tctccgtcta cctcgaagca ctggatcaca agttccacgc cgcagggtta tcagtgctgt
    14821 gcgtgaagcc tggcttcgtc aagacggcca tgaccgcggg cctgaagccg ccgccgtttg
    14881 cgggcgagcc cgagcaggtg gcaaagcagg taatccgcgc gatggatcag cggaaagcct
    14941 tgatttacgc gcccgcgatt tggcggctca ttctgttggt aatccgattc ttgccgcgct
    15001 tcgtgatgag aaagatcaat ttctaaccat ccgtccgccg tgcccgcgct cagcgaccgg
    15061 acaagaacgg cagcatctgg aatagccgct cgggcgtgtt gcgcacgatg ctcataatcg
    15121 gccgccaata ggaggggacg tagacctcgg cccgccgcag gtcggtagcc gcaacgatgc
    15181 ttcgagccac gctttcgggc tgcgcgaata gaatcgattt cttgtgactg accgtcatcg
    15241 gtgtatccac ggggccaagc ttgatggtcg tgatcgtgac gcccgtgccg tacagccgag
    15301 atcggaggcc ttgcaggtat acattgagag cggccttcgc cgtcccgtag gtatagttgc
    15361 gcggccgacc acgctcgccg gccaccgagg tgatgacgca aattcggccg tgccgcgcgg
    15421 cctccatgcg ctggctaagc ggaatcagga gggaaaccac gctcatgaag ttgacgcgga
    15481 gcacgtgctc ggcagcgtcg aaagagcgct ccgtttcgag ctgattgccg agatcaccat
    15541 gtgcgatcaa caccgtatcc agcgctccga ggctccgttc agcccgtgag accagctctg
    15601 cgttcgcgga aaggtcggag aagtccgcgg tctcttgtgt gacttccgcc tccgaactac
    15661 cccgaagccg agcgagcacg ccttgtagct tgtcagcgtt cctagccact aagtgcaggc
    15721 gggcaccgcg gagggcataa agctttgcgg cctcgagcgc gatggcagag gtggcaccca
    15781 agatgagaac gcgttcggtc atcgcgccga aactactacg g
//
LOCUS       DASTKU010000927        10781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_626476, whole genome shotgun sequence.
ACCESSION   DASTKU010000927 DASTKU010000000
VERSION     DASTKU010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10781
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626476"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VFQ61_32730"
     CDS             <1..391
                     /locus_tag="VFQ61_32730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959317.1"
                     /translation="TAVFAAPADFPCSRSNKSALKDFLKNVRSQFETVVFEAGPAWNP
                     DEADDLAEETKTLSSRDPLHHGLSKRKVAYYRLPGPAGHKSRYEDPAIERLAEIAQGG
                     TQQESTYVFTNVDMFADAKRFKRAMGI"
     gene            complement(431..2209)
                     /gene="ptsP"
                     /locus_tag="VFQ61_32735"
     CDS             complement(431..2209)
                     /gene="ptsP"
                     /locus_tag="VFQ61_32735"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET9959318.1"
                     /translation="MSNSDAPPHSVREALVLEGIAGSAGYSIGRAMVVDTRRHGVVRR
                     HIPKHAAEDEVERFDRAVAVAARELREAEAREQNRATRLETSILAAYLLMVEDDTLRA
                     DVERKIRIDLLCAEWALSASVEEMAAQLRTSGDPYLAERSHDFEFVADRILGALSGRL
                     SLLALPFDRAPCVIVAHDLSPTETVGLTRDRVLAIVTEAGTRTSHTAILARALEIPAV
                     VGVSGLLSRIGDNDLVAVDGVHGRIVVNPPPDLVTEHQERSVRFQAAVRARQKLRDRP
                     IVTRCGIPIDLRANIELPSEAEIALSQGARGIGLYRTEFLYVDRSEPPTEDEHYETYR
                     RVVETVAPLPVTLRTFDIGGDKFVSSLQLPHDLNPALGLRAVRLGLARPELLACQLRG
                     MLRASAHGPVRIMVPMIASLGELRAVRELFERARQDLQARGVEHAAQVPLGMMVEVPS
                     AAVMAQQFAREAEFFSIGTNDLVQYALAVDRSSPELAYLASFFDPAILRLIQLVAEAG
                     IQNSRAVSLCGAMASDQLASVLLLGLGLRELSMEAAAIPAVREAIASVTLEEASAAAR
                     HALEAATASEVEQYLLERFSDRLDVE"
     gene            complement(2212..2487)
                     /locus_tag="VFQ61_32740"
     CDS             complement(2212..2487)
                     /locus_tag="VFQ61_32740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET9959319.1"
                     /translation="MSQSKGTFEVQNKLGLHARAATKLVQLASRFPCDIVISRAGQSA
                     NAKSVMGVLLLCGSKGTKLDIEAQGVQAEEAVTELGRLISNRFGEDE"
     gene            complement(2484..3482)
                     /gene="rapZ"
                     /locus_tag="VFQ61_32745"
     CDS             complement(2484..3482)
                     /gene="rapZ"
                     /locus_tag="VFQ61_32745"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET9959320.1"
                     /translation="MNSRPPNSLRGGPVRVLVVTGVSGAGKSTAVHALEDLGFFCVDN
                     LPTPVLSSTVEALSAAGVRRIAFGIDVRVRGFLDDAAAAINGLSIPGEREIEVLFLDA
                     SDEAILRRFSSTRRPHPLSTTLQPGSEVSAVAVLDGIRIERELLTPLRARANAVLDTT
                     RLSVNDLRRQILEHCAPGANSFPRTHVRFVSFGFKFGTPVDADVVLDVRFLQNPHFVP
                     ELRPLSGADEPVRAYVLDGEDTAGFVERARSLLEFCLPRFEREGKSYVTIAIGCTGGR
                     HRSVVLTDHLAHLVEQALGLSIEVVHRDVDRVNMKGLGADPDHANPGTLFGFGGAR"
     gene            complement(3479..4576)
                     /gene="hprK"
                     /locus_tag="VFQ61_32750"
     CDS             complement(3479..4576)
                     /gene="hprK"
                     /locus_tag="VFQ61_32750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HET9959321.1"
                     /translation="MKPHGDPPESIRRVSVRELLDDPLLGLNVRLLAGAGGLSRIIDH
                     PRIQKSGLALVGHLRGVVATRIQVLGETELSYAESLTEEAQQRAAFGLFSQSLSLVLV
                     TRGVEPPRPFRSEAERTGTPLVVCTERSSAVIAAVHALLDQRLAPRTRIHGVLVDVFE
                     VGVLILGKSGIGKSECALELVMRGHRLVADDVIECDYRPPNMVFGQPAALLRHHIEVR
                     GLGVLNIKDLFGVTAIRERKRVDLVVELSLWQPDADYDRIGLDERHRAILGVPVREVV
                     LPVRPGRNMSSIIEIAARNELLRQAGHDSAREFVQRLERGLLRADEPPSHTTSRPPPS
                     SAFRPPLPGLSQPVVNESSAPPPVTPIERPR"
     gene            complement(4573..5052)
                     /locus_tag="VFQ61_32755"
     CDS             complement(4573..5052)
                     /locus_tag="VFQ61_32755"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET9959322.1"
                     /translation="MRLCEILSVDRILVDVDGGYVKNKPEALGRLAEMLSPALGIEKS
                     HVEALLQERERLQSTGIGDGVAIPHTAVEEATAQAAALVLCPRGIDFEAIDGEQVRIV
                     FGVVGPKRATGEHLRTLARISRLLRDANTRSQMLSATEASSVYALIQAQDARSEVKT"
     gene            complement(5092..5532)
                     /gene="raiA"
                     /locus_tag="VFQ61_32760"
     CDS             complement(5092..5532)
                     /gene="raiA"
                     /locus_tag="VFQ61_32760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET9959323.1"
                     /translation="MNISITFRHMASSEAIKSYAADKLGKLQKFLRQPMTAKVTLSVD
                     GLDQTAEARISSGGEHLEAKETNEDMYASIDRMMGKLERQIRGAKGAAQAKRRGAESV
                     RLPPVIGEEEADVAAPARSAKKTVKKAKATAKKATAKASVKKAR"
     gene            complement(5855..7345)
                     /gene="rpoN"
                     /locus_tag="VFQ61_32765"
     CDS             complement(5855..7345)
                     /gene="rpoN"
                     /locus_tag="VFQ61_32765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HET9959324.1"
                     /translation="MEIKQQLRLSQQLVMTPQLQQAIRLLQLSRLELIEEVRKELDNN
                     PVLSEDEPEHRNRVEPGEVRAAPENQPEDPYYKTNDSKNQERASREVDWEKFLENRTL
                     QQPPTASRGGFEELPPIEQNLTKPRSLQDHLLWQLQMSDFTEPERKFAELVIGNLDEK
                     GYLDLIGVERNDGSRTPDLTIEDLAEEAELHPDDAPLVLEMIQNFDPIGVAARNLGEC
                     LLVQARAAGYEEGEAVIEIIKHHLAHLEKHNYQAIAREMRIPIEDVYEAAKEIQSFES
                     RPARNFTDQDERTIGITPDVYVIKDGTEFVVLDNDRGVQRLFINENLTKRLMKDPSAK
                     EFIGEKLRNAQWLIRAIEQRRKTIIRVAECIVEKQRDFFEKGVAFLRPMILRDVAESV
                     GMHESTISRVTTNKYMHTPQGLFELKYFFNSSIRRVANEDIASESVKQAIKKIIDDED
                     KGNPVSDQQIVEMLEKAEGIKIARRTVAKYREMLGILASSKRKRLF"
     gene            complement(7525..8028)
                     /locus_tag="VFQ61_32770"
     CDS             complement(7525..8028)
                     /locus_tag="VFQ61_32770"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HET9959325.1"
                     /translation="MVKLSNNGTRRWLLGSAVAALVAGPAAAPLAQAGPLAVIAGETL
                     DVKADSLEVDMARGTAVLDGKVHASLGDLEVDCAKVEVRYDQAPNVRWAKGTGGVSAR
                     AKGFAATAAAFEVDVARRSVRLEGTVRLSRGRGWVTADSATIDLSTKKVVLNDVQGSI
                     PVQTPAR"
     gene            8451..10145
                     /locus_tag="VFQ61_32775"
     CDS             8451..10145
                     /locus_tag="VFQ61_32775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9959326.1"
                     /translation="MQRRASNAKTAASRLKTLYLYVRDRFGPAEADVFLQRTRLDRDF
                     LADETRPVPAELWHSALVEFSARCGQDAILDTVHAVVHPENLGVWSHVLRGANDVSSA
                     YRRLEQFGGEQIWTERWRTLEQSPGHWKGVIRTALDPGLERDGLCSLARQAELAGLPL
                     LFGLAPAQVSVRFADSTSPSTVSLEFEARWHEKPVQGIVVVGTAAGAAAGVGLAGLGV
                     FSSWSAAGLISALLGFGAGVLGMHEVKRRTQSAAQMMRIRALERSATLRESRDHGAVG
                     FQAGLVVAGQYRLTEKLGTGANGTIWEAEHLADGGLFAIKLLRAAVAHDTVAADRLRR
                     EAAVLGLAWHTNVVEVFDEGHLPDGTSYLVMERLYGESLAVRLKRRGRLSPGEILPIA
                     LQVCDALGAVHAAGIVHRDVKPSNIFLALDEADGTSATPREQAKILDFGVARVEWAET
                     RLTNADIPLGTPGYMPPEQEQGLEVDARSDVYALGATLFECLSGHPPPSRSGIVQHVD
                     PTDSESGVQPALRAIPEEWRSIIERAMALNPRERFQDTKSFRDALLATGEARPQSA"
     gene            complement(10167..>10781)
                     /locus_tag="VFQ61_32780"
     CDS             complement(10167..>10781)
                     /locus_tag="VFQ61_32780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959327.1"
                     /translation="ILVGAVVHLLVLILVGGDWMPFYRLATPVLPGLAYVAAELARNS
                     PPWILALRGAAQAVVAGMLFTALGPSARNVFEQRLALIEAIEPELVGARRIAALDIGW
                     LSAATSASVFDAAGVTDPSVARLPGGHTTKRLPEGWLEQHRIDSLVLLADAPDPASFP
                     ELRYARVVEARIARMAGIEGFKPVRVLPLLGTKQSYIVARRQTD"
BASE COUNT         1985 a         3472 c         3332 g         1992 t
ORIGIN      
        1 gacggccgtg ttcgctgcac cggcagattt tccgtgcagt cgttcgaata agtccgccct
       61 caaggatttc ctgaagaacg tccgcagcca attcgagacg gtggttttcg aagccggccc
      121 cgcttggaat ccagacgagg cagatgacct agccgaggag acgaagacgc tttcctcgcg
      181 agatcccctg caccatgggc tatcgaagcg aaaagtcgct tactatcgct tgcccggtcc
      241 ggctggccac aagtcgagat acgaagaccc ggcgatcgag cggctcgctg agatcgcgca
      301 gggtggcacc cagcaggagt cgacgtacgt cttcacgaac gtggatatgt tcgcggacgc
      361 aaagcgcttc aagcgcgcga tggggatctg acccacaaca gtcgagcgcc gcgcgtaaat
      421 cgacgcgacc tcactccacg tcgagccgat cgctgaaccg ctccaggagg tactgctcga
      481 cctccgaggc ggtagcggct tcgagcgcgt gccgggccgc agcgctagcc tcctccagcg
      541 tcaccgaggc gatcgcctca cgaaccgcgg ggatcgctgc cgcctccatg cttagctcac
      601 gcaagcccag tccgagcagc aataccgacg cgagctggtc actggccatg gcaccgcaca
      661 gtgaaaccgc gcggctgttc tggatgccgg cctcggccac cagctgaatc aatcggagaa
      721 tggcggggtc gaagaacgag gcgagatatg cgagctcggg gctcgagcga tccaccgcga
      781 gcgcgtactg caccaagtca ttggtgccga tgctgaagaa ctcggcctcg cgcgcgaact
      841 gttgtgccat gacggccgcg gaggggacct cgaccatcat ccctagcggc acctgagctg
      901 cgtgttccac gccacgcgct tgcaggtctt ggcgggcgcg ctcgaagagc tcgcgaaccg
      961 cgcgaagctc accaagggaa gcaatcatag gcaccatgat gcgcacgggc ccgtgcgcgg
     1021 aggcgcgcaa catgccccgc agctgacaag ccaagagctc ggggcgagcc aatccgagcc
     1081 gaaccgcgcg caggcccaaa gccggattca aatcgtgcgg cagctgtagc gaggaaacga
     1141 acttgtcacc cccgatgtcg aacgttctga gcgtaaccgg caacggcgcg acggtctcta
     1201 ctacccggcg gtaggtttca tagtgctcgt cctcggtcgg tggctccgaa cggtcgacgt
     1261 acaaaaactc cgtccgatac agtccgatac cgcgagcgcc ctgcgaaagc gcgatttcgg
     1321 cctcgctcgg gagctcgatg ttggcgcgta gatcaatcgg gatcccgcag cgggtaacga
     1381 tcgggcgatc cctgagtttc tggcgcgcac gcaccgccgc ttggaaccgc accgagcgct
     1441 cctggtgctc ggtgaccaga tccggaggtg gattgacgac gatgcgacca tgaacgccgt
     1501 ccacggccac caagtcattg tcaccgatcc gagacaacaa cccagagacc ccgacgaccg
     1561 ccggaatctc gagcgcacgc gccaagatcg cggtgtgact ggtgcgcgtc ccagcctccg
     1621 tcacgatcgc cagtacgcga tcccgagtga gccccacggt ttcggttggg gacaagtcat
     1681 gcgccacgat cacgcacggc gcccgatcga atggcaacgc gagcagcgac aagcggccgg
     1741 agagagcgcc gagaattcgg tccgccacga actcgaagtc gtgacttcgc tcggcaaggt
     1801 aaggatcgcc ggacgttcgc agctgggccg ccatttcttc cacgctggcg gacagcgccc
     1861 attccgcgca aagcaggtcg atccgaatct tgcgctctac atctgcgcgc aaggtgtcat
     1921 cttcgaccat caacaggtaa gccgcgagaa tcgacgtctc gaggcgagta gcacgattct
     1981 gctcgcgcgc ttcggcttcg cgcagctccc gcgccgccac cgcgaccgcg cgatcgaagc
     2041 gttctacctc gtcctccgcc gcgtgcttcg gaatgtggcg gcgcactact ccatggcggc
     2101 gagtgtccac caccatagcg cgcccgatgg agtagcccgc agagccggca atcccttcca
     2161 ggaccagcgc ttctcgcacc gagtgcggcg gagcatcgct gttgctcatc gttactcgtc
     2221 ttctccaaaa cggttggaga tcaagcgccc gagctcggtc acggcttctt cggcctgcac
     2281 gccttgcgcc tcgatgtcga gcttggtgcc cttcgacccg cagagcagca gcacgcccat
     2341 cacgctcttc gcgttcgccg actgaccggc acggctaatc acgatatcgc acggaaaacg
     2401 cgacgcgagc tgcaccaact tggtcgccgc ccgtgcgtgc aaaccgagct tgttctgcac
     2461 ttcgaaagtg cccttcgatt ggctcatctt gcgcctccga aaccaaatag cgtgccgggg
     2521 ttagcgtgat ccggatcggc acccagaccc ttcatgttga cgcggtccac atcgcgatga
     2581 actacctcga tgctgagccc gagcgcctgc tcgaccaggt gcgccaggtg gtcggtgagc
     2641 acgacggagc ggtgacgccc tccagtacag ccgatggcga tggtcacgta gctcttccct
     2701 tctctctcga agcgaggcag gcaaaattcg agcagcgaac gcgcgcgctc cacgaagcca
     2761 gccgtatcct cgccgtccag aacgtatgca cgcactggct cgtctgcgcc gctcagtggc
     2821 ctgagctcgg gcacgaagtg cgggttttgc aggaagcgga cgtccaagac tacgtcggcg
     2881 tccaccggtg tgccgaactt gaagccgaaa gacacgaagc gcacgtgggt gcgcggaaag
     2941 ctattcgctc cgggtgcgca gtgctccagg atttgccgac gcaggtcgtt gaccgaaagc
     3001 cgcgtcgtat ccaggacggc gttagcgcgg gcgcgtaacg gcgtcagtag ctcgcgctcg
     3061 atacggatgc cgtcgagcac cgcgacggcc gagacttcgc tacccggttg cagggtagtg
     3121 ctcaacggat gtggtcgtcg cgtgctgctg aagcgccgca ggatcgcctc atccgaagca
     3181 tcgaggaaca ggacttcgat ctcccgctca ccggggatgg acagaccatt gatggcagcc
     3241 gcggcgtcgt ccaggaagcc gcgcacgcgc acgtcgatcc cgaacgcgat gcgccgcacg
     3301 cccgccgcgg acagagcctc gaccgtgctg ctcaggacgg gtgtcggcag gttgtcgacg
     3361 cagaagaacc cgagatcctc cagcgcgtgc acggcggtcg atttgccagc cccgctgacg
     3421 ccagtcacga ccaacacccg taccgggccg cctcgcagcg agttgggtgg gcggctgttc
     3481 atcgcggcct ctcgatcgga gtcaccggcg gtggcgcgga gctctcgttg acgacgggct
     3541 gcgacagacc cggaagcggc ggtctgaatg ccgaacttgg cggcggacgc gaggtcgtgt
     3601 gtgaaggcgg ttcatccgcg cggagtaggc ctcgttcgag gcgttgaacg aactcgcgcg
     3661 ccgagtcgtg gccggcctga cggagcagct cgttccgcgc cgcgatctcg atgatgctgc
     3721 tcatgttgcg gccgggccta accggcagca ccacctcgcg caccggcaca cccaagatcg
     3781 cgcggtgacg ctcgtcgagc ccaatgcggt cataatcggc atcgggttgc cacaaggaaa
     3841 gctccaccac caagtcgacg cgcttgcgct cgcgaatggc ggtcaccccg aacaagtctt
     3901 tgatgttgag cacacccagc cctcgcacct cgatgtgatg gcgcagcaag gccgccggtt
     3961 gtccgaagac catattggga ggtcgatagt cgcactcgat cacatcgtcc gcgaccaagc
     4021 ggtgcccacg catcacgagc tcgagcgcgc actcgctctt gccgatgccg ctcttgccca
     4081 aaatcagtac gcccacctcg aacacgtcca ccaggacgcc gtggatacgc gtgcgcggtg
     4141 ccaagcgttg atccaggagc gcatgcacgg ccgcgatcac ggccgacgac cgctcggtgc
     4201 agacgaccag cggcgttccg gtgcgctcgg cttcgctgcg gaacggccgc ggcggctcta
     4261 cgccccgggt gaccaaaacc agggacaggc tctgggaaaa caacccgaat gccgcgcgct
     4321 gctgcgcttc ttccgtcaag gactccgcgt aactcagttc ggtttcgccg agtacttgga
     4381 tacgcgtcgc aaccaccccc ctgaggtggc ccaccagagc caagcccgac ttctggatcc
     4441 gcgggtgatc gatgatccgc gacagccccc ccgcgcccgc caacaggcgc acgttgaggc
     4501 cgagcagagg gtcatccagc agctcgcgaa ccgagacacg tcggatgctc tccggcgggt
     4561 caccatgcgg tttcacgtct tgacctcgga ccgcgcgtcc tgggcttgaa tgagcgcgta
     4621 cacgctcgaa gcttccgtcg cggaaagcat ttgagatcgc gtattggcat cgcggagcag
     4681 gcgggagatt cgcgccaggg tccgcaagtg ttcgcccgtc gcacgcttcg ggccgacgac
     4741 accaaacacg attcgaacct gttcaccgtc aatggcctcg aagtcgatcc cccgcgggca
     4801 cagcaccaag gctgcggcct gcgcggtcgc ctcctccacg gccgtatgag ggatggcgac
     4861 accatctccg atccccgtgc tctgcaggcg ctcgcgctcc tgtagcaggg cctcgacgtg
     4921 agatttctcg ataccgagcg cgggagacaa catctctgcc agccgcccca aggcttctgg
     4981 cttgttcttc acgtacccgc catcgacgtc gaccaggatc ctgtcgacgc tgagaatttc
     5041 gcaaagtcgc atgctgggct ccatcggcaa gcagccggga agtcgctgcc gctaacgcgc
     5101 cttcttgact gaggctttgg cagtcgcctt cttcgcagtc gccttggcct tcttcaccgt
     5161 ctttttagcg ctccgggcgg gtgccgcaac gtcagcctct tcctcgccga tgacgggcgg
     5221 caatcgaaca ctttcggccc cgcggcgttt ggcttgagcc gcgccctttg cgccccggat
     5281 ctgacgctcg agcttgccca tcatgcggtc gatggaggcg tacatgtcct cattggtctc
     5341 tttggcctcg aggtgttcac caccgctcga gatgcgagcc tcggctgtct gatccaaacc
     5401 gtccaccgag agcgtgacct tcgccgtcat gggctgtcgc aagaacttct ggagcttgcc
     5461 aagcttatca gcagcgtagc tcttgatggc ctcgctggat gccatgtggc ggaaggtgat
     5521 gctgatgttc attcgagcct cctgtcggtg tcggaaccga cctcgaccgc acattagccg
     5581 aacgaacgat tgcgaggcga gatcgacgac ggccccgagc gcgctttctg cccgctaccg
     5641 tcgttgtctc ccccgttggc cgccttccgg gttcgccggg gctaggtccg agagcgtcgg
     5701 cctagggccg cgagagtcgg aaaccgacca ggcgccttcg aactccctgg cccttcatcc
     5761 gccgtgttcg gctcgacggc aaacgaccga ccgcgcacgc ccgtgcgcaa gttgcttgcc
     5821 gcgcaccttc gtcccaacgc tcacgagacc ccaatcagaa cagccgcttg cgcttggacg
     5881 aagccaaaat tccgagcatt tcacggtact tagcaaccgt tctccgtgcg attttgatcc
     5941 cttcggcttt ctccaacatc tcgacgatct gctgatcact gacggggttc cctttgtctt
     6001 cgtcgtcgat gatcttcttg atggcctgtt tgacgctctc gctggcgatg tcctcgttgg
     6061 ctacgcggcg gatgctcgaa ttgaagaagt acttcaattc gaacagcccc tgaggggtgt
     6121 gcatgtactt gttcgtggtg acgcgggaga tagtggattc gtgcatgccg accgactcag
     6181 ccacgtcccg aaggatcatc ggccgtagga aggcgacgcc cttctcgaag aagtctcgct
     6241 gtttttcgac gatgcactcc gccactcgga taatcgtctt gcgtcgttgt tcgatggcac
     6301 gaatcaacca ctgcgcgttg cgcagcttct caccgatgaa ttccttcgcg ctcggatcct
     6361 tcatcaagcg tttggtcagg ttctcgttga tgaagagccg ctgcacaccg cggtcattgt
     6421 cgagcaccac gaactcggtg ccgtccttga tgacgtacac gtccggcgtg atgccgatgg
     6481 tgcgctcgtc ttggtcggtg aaattacggg ccgggcggct ctcgaagctc tgaatttctt
     6541 tagccgcttc gtagacatcc tcgatgggga tccgcatctc ccgcgcaatc gcctggtagt
     6601 tgtgcttctc caggtgagcg aggtgatgct tgatgatttc gatcaccgcc tcgccttcct
     6661 cgtaccccgc cgcgcgcgct tggaccagta agcactcgcc caggttgcgg gcggcgaccc
     6721 cgatcggatc gaagttctgg atcatctcga gcacgagcgg ggcatcgtcg ggatgcagct
     6781 cggcttcttc agcgagatct tcgatggtca aatcgggcgt gcgcgaccca tcgttgcgct
     6841 cgacgccgat caagtcgagg taaccctttt cgtcgaggtt accgatcacg agctcggcga
     6901 acttacgttc cggctccgtg aaatcgctca tctgcagctg ccacagcaag tggtcctgca
     6961 ggctacgagg cttcgttaaa ttctgttcga tcgggggaag ttcttcgaaa cctccgcggc
     7021 tcgccgtcgg cggctgctgt aacgtgcggt tctcgagaaa tttttcccag tcgacttcac
     7081 gcgacgcgcg ctcctgattc ttggagtcgt tcgtcttgta ataggggtct tcgggttgat
     7141 tctcgggggc tgccctcacc tccccgggct ctacccggtt tcggtgttcc ggctcgtcct
     7201 cggagaggac ggggttgttg tcgagctcct tgcggacttc ttcaatgagc tccaatcgag
     7261 aaagctgcag caggcgaatc gcctgctgca gttggggcgt catgacgagc tgctgggaga
     7321 gcctgagctg ctgcttgatc tccatctaag acccgggctt ggggaccctg aattccttcc
     7381 aagctcgccc agtttaacat cacgaagcaa aaagtgggcc ttccccaacg gctctttcag
     7441 ctttttcctc aagccacaaa acggccggcc tctcacagag cgaccagaca cgataggatt
     7501 cgcgctatgg gcgaaaggat tgcactatcg ggcgggcgtc tgaacgggaa tagagccctg
     7561 cacgtcgttc agaacgactt tcttggttga cagatcgatc gtcgcgctgt ccgccgtcac
     7621 ccagccacgg cctcggctta accgaacggt cccttcgagt cgtacgcttc ggcgcgcaac
     7681 atccacctcg aacgcggccg cggtggcggc aaaacctttg gcgcgtgcgc tcactccacc
     7741 cgtgcctttg gcccagcgta cgttgggggc ctgatcgtac cggacttcca ctttggcgca
     7801 gtccacctcg agatccccca gagaggcgtg cacctttccg tcgagcactg cggtgccgcg
     7861 agccatatcg acctcgagcg aatccgcttt tacatccagc gtttcccccg cgatcaccgc
     7921 aagcggccca gcttgcgcta ggggtgcggc agcgggaccc gcgacgagcg cagctacggc
     7981 ggagccgagc agccatcgac gggtgccgtt attcgaaagt ttcaccacga gactctagca
     8041 ttcctacgta gcacagacca cgcaccttcc tctcgccgat gtgatgtatc gcacgcattt
     8101 cgaacgggtt tattgggtgg tctcgggaag aacgtgcgcc gcgccggcac ctcttggttc
     8161 aggtcagtcc gagccgatcc gacaacagaa cggagccgga tcgttcagca gggccgtact
     8221 gtgtcgacgc tttgcacacg agcgtcagag gattgggtga cgctgcgtga ccagctcgcg
     8281 agatgaccta ctggttcgtg agggaagacg cgcttgctcg agtgaaagcg ccttgacttg
     8341 gccagctagg gctcacctca cgccgagaaa gccctgcaat ttcgacggaa gaagctgcgc
     8401 cgggtcacgc tcgacttgac cggcgccgcg aggtccggaa atgtaacggg gtgcagcgtc
     8461 gggcctcgaa tgcaaagacg gccgcgtcac gcctgaagac gctctatctg tatgtccgcg
     8521 atcgcttcgg acctgcagaa gcggacgttt ttctgcaacg cactcggctg gatcgggact
     8581 tcttggcgga cgaaacgcgc ccggtccccg cggagctttg gcactccgcg ttggtcgagt
     8641 tcagcgcccg ttgtgggcaa gatgcgatct tggacacggt gcatgccgtc gtacatccag
     8701 aaaatctggg ggtgtggtca cacgtgctcc ggggcgcgaa cgacgtcagc agcgcctatc
     8761 ggcgcctgga gcagttcggc ggagaacaaa tctggaccga gcgctggcgc accctcgagc
     8821 agagccccgg ccactggaaa ggcgtgattc ggactgcgct cgacccaggg ctcgagcgcg
     8881 acggactatg ttcactcgct cgtcaggccg agctcgccgg gctgccgcta cttttcggcc
     8941 ttgcaccagc gcaggtcagc gttcgttttg cagatagtac tagtccttct acggtgagcc
     9001 tcgagttcga ggctcggtgg cacgaaaagc ccgtgcaagg catcgtggtc gtgggaacag
     9061 ccgcaggagc ggccgcgggc gtaggtttgg cggggctggg tgtgttcagc tcgtggtcag
     9121 cggctgggct gatttctgct ctgctcggct tcggtgcggg cgtcttgggc atgcacgagg
     9181 tgaaacgtcg aacgcaaagc gccgcgcaaa tgatgagaat tcgcgcgctg gagcgatcgg
     9241 ccactctgcg tgaatcgcgg gatcacggcg ccgtgggctt ccaagctggc ctcgtggtgg
     9301 ccggacaata tcgtctgacg gaaaaactcg gcacgggcgc caacgggacg atctgggaag
     9361 ctgagcattt ggccgatggt ggcttgtttg ctatcaagct gctgcgcgcc gccgtggctc
     9421 acgataccgt cgcggcagat cgcctgcggc gtgaagccgc ggtactcggc ttggcctggc
     9481 acaccaacgt agtcgaggtc ttcgacgaag gccacctgcc ggacggcacg agctacctgg
     9541 tgatggagcg actctacgga gagtcgcttg cggtacggct caagcgacgt ggccggcttt
     9601 cgccaggaga gatcctgccc atcgcgctac aggtttgcga cgcgctcggt gcagttcacg
     9661 cagcgggcat cgtccaccgc gacgtcaagc ccagcaatat cttcctggct ttggatgaag
     9721 cggacggaac gagcgcgacc ccgcgagagc aggccaagat tctggatttt ggcgtggcgc
     9781 gcgtcgagtg ggcggagact cgcctcacca acgcggacat tccgctcggc actcccggct
     9841 atatgcctcc cgagcaagag caagggctcg aggtggacgc gcgaagcgat gtctatgctc
     9901 tcggtgcgac gttgttcgag tgcttgtccg gccacccgcc gccctcgcgt tcgggcatcg
     9961 tgcagcacgt ggatccgacg gacagcgaga gcggagtgca gccggcgctc agggctattc
    10021 cggaggagtg gcgcagcatc atcgagcgcg cgatggcgct caacccgcgc gaacgcttcc
    10081 aagacactaa gtccttccgc gacgctctac tagcgaccgg cgaggcccgc ccgcagtccg
    10141 cctagctcga tttggggctc gagtcgtcag tcggtttggc gtcgagcgac gatgtagctt
    10201 tgcttggtgc cgaggagtgg cagcacgcga acgggcttga agccttcgat cccggccatc
    10261 cgcgcgatgc gggcttccac gacgcgagcg tatcggagct cgggaaatga cgcaggatca
    10321 ggcgcatcag cgagcagaac cagcgagtcg atccgatgct gctcgagcca accttcgggc
    10381 aagcgcttgg tcgtatgtcc accgggcaga cgagcaacac tcggatccgt gacaccggcc
    10441 gcgtcgaaaa cgctcgctga agtggccgca gaaagccacc ctatgtccaa ggcggcgatt
    10501 cgacgcgccc cgacgagctc aggttcgatt gcctcgatca atgccaggcg ttgctcgaag
    10561 acattccgtg cgctcggtcc caaggcagtg aagagcatac ccgcaaccac ggcttgggct
    10621 gccccacgca gcgccaggat ccaaggcggc gaattacgag cgagctccgc cgcaacgtaa
    10681 gccaagcccg gaagcacagg cgtcgcaagc cggtagaaag gcatccagtc acccccaacg
    10741 agaatgagga cgagcaagtg gaccactgcg ccgacgagga t
//
LOCUS       DASTKU010000928        13009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1969210, whole genome shotgun sequence.
ACCESSION   DASTKU010000928 DASTKU010000000
VERSION     DASTKU010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13009
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969210"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFQ61_32785"
     CDS             <1..326
                     /locus_tag="VFQ61_32785"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9959328.1"
                     /translation="SISLAPYAQVPASVPNSTKGALNTFTRSLALELAKERIRVHAVA
                     PGIIRTPLHGRAEGEYDELAALQPVGRIGDVADVVMAVQALVRQSFATGVVLPIDGGS
                     SLGHW"
     gene            complement(333..1301)
                     /locus_tag="VFQ61_32790"
     CDS             complement(333..1301)
                     /locus_tag="VFQ61_32790"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9959329.1"
                     /translation="MPNSKRRAPARQRVSGRVGAAPAALDDLGALSWFVRAVEAESFS
                     SAARRAGTTTSAISKRIAGMEERLGVRLFERTTRRAQTTDAGRALYEHARRLLEEATR
                     AEEAVSAYRGQLVGRIRVSAPVTFGQMHIAPLVGAFLEQHPGLSIALSLNDAFVDLLA
                     SGVDVAVRSGRSGDSSLLSKKLASDARVVCASPKYLAKHGTPRVPEDLLRHACLRHSM
                     MQPGAGWLFETPAGPTTIAVAGQIEVDHVGTLRDAALASRGIVYLPRYAVVQDLKAQR
                     LVSLLAEFVPEPAAIRALWAAGKQPVRRVNALVEFLAIELPKRLDD"
     gene            complement(1310..1867)
                     /locus_tag="VFQ61_32795"
     CDS             complement(1310..1867)
                     /locus_tag="VFQ61_32795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HET9959330.1"
                     /translation="MTPRNLRSREALVDWIEAGGVADYTFFWGHGASPADGIGKNCLS
                     QWYGSEFEIDGVRYATAEHYMMAEKARLFADTESVRAILAAPHPADAKRLGRSVRNFA
                     ESTWCEHRMRIVVRGNLAKFGQHAALLEFLRGTQDQVLVEASPADRVWGIGLDAGHPH
                     AREPKQWRGLNLLGFALMEVRSALA"
     gene            complement(1993..2868)
                     /locus_tag="VFQ61_32800"
     CDS             complement(1993..2868)
                     /locus_tag="VFQ61_32800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959331.1"
                     /translation="MKSTYGFLASLTAFGLTLSVSQPARAGLAECGYIDLEARSSCEL
                     RTGIECTGECTAPSFQAACAAQLEASCEGSCSASASVQCVGQCEADCQAECAVNPPRL
                     DCQARCEADGEAECNAQCSSSSNRSRCQASCKASFSAQCEASCQGQPGSATCQGKCEA
                     SCEGSCRAQANARCQVNCQASGFVQCEARLKGRCEIDCSSSGDGALYCNNQYIDHGGH
                     LDRCLDALAAIDVVVQGSAQGRCSNGRCQGSAEGSVSCALSKPGSEANSAGATTVYGL
                     CLALGLAAAYRRRRH"
     gene            complement(3194..4174)
                     /locus_tag="VFQ61_32805"
     CDS             complement(3194..4174)
                     /locus_tag="VFQ61_32805"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9959332.1"
                     /translation="MRAVVMLEHGGPEVLKLSEQPAPEPGEFDLLVEVHATSVNPVDA
                     KVRRAGAGRKFPLILGYDVSGTVVACGARVVGFAPGDEVYGCPSLFGNGANAELVLLD
                     ARSAAKKPNSLDHATAACLPLVSLTAWEALHERARIEPGETILVHAGGGGVGHIAVQL
                     ARLHGCKVLTTAGRPESIAFCSEELGADEVIDYSSNDFVARVSELTHGKGLRCVFDTV
                     GGEVFERSIECVAPCGQLVTILDSKPGARASALLYRSITVHYEFMGARVAYGEHPERQ
                     GAILAGIASLVDRGLLRPHVSDRFALESVADGHRQIETGHTLGKVAIAVR"
     gene            4398..5087
                     /locus_tag="VFQ61_32810"
     CDS             4398..5087
                     /locus_tag="VFQ61_32810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9959333.1"
                     /translation="MHTSALRVLLVDDDEETLELVGKALTREGHEVLCARTAEQANQL
                     LAQVPIEILVLDVMLQQGSGLELCAELRQRGNQVPILFLSARGTVNARVEGLEVGGDD
                     YLTKPFAVREVVARVRALGRRRPLTRDMHVSVGSLTLHFAERRAVAHDQQIPITRREW
                     DILRALGNARGRVVAFDELLEQAWGEVTEKGRASLEVIMSRLRRKIDAAAGRPLIRTV
                     RGHGYALELDG"
     gene            5084..6433
                     /locus_tag="VFQ61_32815"
     CDS             5084..6433
                     /locus_tag="VFQ61_32815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9959334.1"
                     /translation="MTRRRSIQQSLRHTYLLSIATIGASLVGLGALSATYVALLRDDA
                     NSRALAQLLGGELREHAQDSLSVQREALRRELEEQRWFERETEVWRERVLIAGTRPEA
                     ELARWTTLLGCTTARLHGAWHRICGARPDPSVAVVVASPVGPVFRAQLPVVAAISIAT
                     ALAVLGFSLLSRLLVHRSLDPLARFESAMARLPAKPGQRLQSEWGAREINHLAQTSNA
                     LLARLDEIVEREQAFAANAAHELRSPLTRLRGQLELLLDEPHARATNPSAAELRNRLE
                     RAAKTCTELVNCTEALLALARREASLHEAVNWEEVALSICDNLSDQERARVSVNAQEA
                     LVRGDLALLGLAAQNLVDNALKYSAGPIELNVLAAEKRCSFVVVDCGPGISSSELDRV
                     RQPFVRGSSNVRGTGLGLALVEHVAKLHGGELSLKNRSERSGLIAALSLPRWSPSRP"
     gene            6586..7347
                     /locus_tag="VFQ61_32820"
     CDS             6586..7347
                     /locus_tag="VFQ61_32820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9959335.1"
                     /translation="MTFIQDRCPPEDGKSGPSRKPEAPIRRSMARILLIDPDEQARLL
                     VGCALSAAGYELVSETTTGEALASVRRAPPQLVISECGAPGVELEALLQFFSDAGVLT
                     GLPILVLSERASEADRVAAFESGVDDFVAKPFSPRELVLRVRALLRRCGDSAETGATW
                     QHGALCVDVDGHRTWVEGREVQLTATEFQILVTLRTNRGRVMSRERIMAGLWGGARVG
                     SRTIDAHVKRLRTRLGAASSCVETVRGVGYRFVEP"
     gene            7420..8553
                     /locus_tag="VFQ61_32825"
     CDS             7420..8553
                     /locus_tag="VFQ61_32825"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET9959336.1"
                     /translation="MRASSLWSVFLLVACQKAEAPENPAPAQPAQPAPPAHKQVALQD
                     TSFKRPSAERIIAIGDLHGDIRAAQAALRLGGALGPDGHWAGGKLVVVQTGDQLDRAD
                     EEPEILELLDRLAAEAPAAGGALYTLNGNHEVMNVQGDFRYVTEDGFRDFAATPLDGL
                     HTREIRSLPSDQQGRAAAFLSGEVVAKRLAQRPVVIQVGDSVFVHGGLLEAHVDYGLG
                     RINRETAQWMQAPDSKPAPAILTEERSPIWLRAYSDGIPPAGVCQELGRVLDRLEAKR
                     LVMGHTVQRQGINSVCRGRAWRIDVGLSRAYGQNPPSVLEISGDTVKVLQGDLTLLTT
                     PAAPSAPIPKPSPSTKAPVTKSSSSPNKPKAQPAKNSPAPASP"
     gene            complement(8591..10420)
                     /gene="glmS"
                     /locus_tag="VFQ61_32830"
     CDS             complement(8591..10420)
                     /gene="glmS"
                     /locus_tag="VFQ61_32830"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET9959337.1"
                     /translation="MCGIVGYIGSRNVTPILLDGLRKLEYRGYDSAGLAVHTAHGLDV
                     VRAVGKLDNLERAASARQLNGTLGIGHTRWATHGRPTEPNAHPHVAGDVAVVHNGIIE
                     NHLELKAELEGSGVAFASDTDTEIVAHLVHAELARHPVLFDAVRAALSRIRGAYALAI
                     VSASRPDSIVVAKSASPLVIGLGDGETLCGSDIPALLSQTRRMLFLEDGEMAELTRDG
                     VRIETLEGERVQREPKRIDWSPIQAEKGGYKHFMLKEIFEQPSALSDTLRGRIDIAEG
                     ELVDAEIGFGAELARRVSRVCLLACGTSYHAALAGRNWIEKYARVPAQAELASEVRYR
                     DPVFTPADLVIAISQSGETADTLAALRTAREAGASVISVVNVVDSAIARAAQGTLYTH
                     AGPEIGVASTKAFTTQLAALLMIAVHLGRRRGSLPPDRAREVLQALFEAPGLMRTALE
                     SHVHVQEVARRWQHASHMLFLGRGAGFPIALEGALKLKEISYAHAEGYAAGEMKHGPI
                     ALIDEAMPVVVVAPKDTQYEKMLSNVQEVRARDGRVIAVITEGDASLGKLADFTLTVP
                     AAPEVIQPLLTVIPLQLLAYCIADLKGTDVDQPRNLAKTVTVE"
     gene            10806..11030
                     /locus_tag="VFQ61_32835"
     CDS             10806..11030
                     /locus_tag="VFQ61_32835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959338.1"
                     /translation="MSAASTASKTRYRYPIHAVILVVAAWLFLLALLAGYATVAIALF
                     PMSPIVAIGAACLIGSVHSYAVSVRETVRD"
     gene            complement(11088..11963)
                     /locus_tag="VFQ61_32840"
     CDS             complement(11088..11963)
                     /locus_tag="VFQ61_32840"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9959339.1"
                     /translation="MQRVGFIGLGIMGSGMVRNLSKKGVPVTVWNRSPERAAGLGVEV
                     PVARSPRELAQDVDVIVCCVSNPQAVEQVVFGAEGVLAGARAGLRYVECSTVSPELVR
                     RLAKAFSERGAGFLEAPVTGSKNAAENGTLLFMSGGPAELNSELEPLLLKMGSRVIHC
                     GENGQGALMKLAGNTLIAFMLEGLCEAMTVATKGGLSPEKILEVIAASGFASPYYAFK
                     GNAIRERNFETHFSVDLLVKDLTLMLQEAATQPTAMPGLAAIREQFQTARALGLGERD
                     IAAVIQTLESTSRGR"
     gene            complement(12213..>13009)
                     /locus_tag="VFQ61_32845"
     CDS             complement(12213..>13009)
                     /locus_tag="VFQ61_32845"
                     /inference="COORDINATES: protein motif:HMM:NF022812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HET9959340.1"
                     /translation="QTQLGSWAELRHDNILYGKQSYTGSVLCEYPTGYVEPYPEFYQR
                     LGEVMRGLSLRLAALPYSKEEGEVSLRKHWGDLLQRFDETLARLQTMARKELAAEPFS
                     SDEKRFLKNTIEVEMVGCGSPTFHGWYASLLYGSSPLAFKPVIADVHTDPDSNQVLEV
                     GTGRANYLAVAIDNESHRGVYVGPVYSYYEFTTESSHRMTDEEWTSQLMYSKPPAAPT
                     WVRSFQPAATEREFDMEQKTPANPGRPRGRRPVDRGDPLGPSPTSE"
BASE COUNT         2334 a         4422 c         3982 g         2271 t
ORIGIN      
        1 cgtcgatctc gctggcccct tacgctcagg tgccggcgag tgtaccgaac tcgaccaaag
       61 gcgcgctgaa cacgttcacg cgttcgttgg ccctagagct tgcgaaggag cgaattcgtg
      121 ttcacgcggt agcgcccggc atcattcgca cgccgctcca tggccgggcc gaaggcgaat
      181 acgatgagtt ggccgcgctg cagccggtgg ggagaatcgg ggatgttgcg gacgtggtca
      241 tggccgtcca agccctcgtt cgccagagct ttgccaccgg agtggtatta ccgatcgatg
      301 gcggttcgag cctcggacac tggtgaggcg cgtcagtcat cgagacgctt cggcagctcg
      361 atggccagga actcgaccag cgcgttcacc cggcgcacgg gttgtttgcc ggccgcccac
      421 agagctcgta tcgccgcggg ctcggggacg aattcggcga gcagactcac gagtcgctgg
      481 gcctttaggt cctgaacgac ggcgtagcgg ggcagataga cgatgccacg cgaggcgagg
      541 gctgcatcac gcagcgtgcc cacgtggtcg acctcgatct gccccgccac cgcaatcgta
      601 gtgggccccg cgggtgtctc gaacaaccag cccgcccccg gctgcatcat gctgtgccgc
      661 agacaagcat ggcgcagcaa atcctcgggg acccgcggtg tgccgtgttt ggcaaggtac
      721 ttcgggctcg cgcacaccac acgcgcatcc gaagccaatt tcttcgacaa cagactggag
      781 tccccgctgc gaccggaacg caccgccacg tccacgccgc tcgcgagcag atcgacgaag
      841 gcgtcgttga gagagagcgc gatgctcaac cctgggtgct gctccaggaa cgcaccaacg
      901 agcggcgcga tgtgcatttg tccaaacgtg accggggcgc tgacgcgaat gcgtcccacg
      961 agttgcccgc gataggcact caccgcctct tccgcccgtg tggcctcttc gagcaggcga
     1021 cgcgcgtgtt cgtagagcgc ccgcccggca tcagtggtct gcgctcgccg cgtggtgcgc
     1081 tcgaagagcc tgaccccgag ccgctcttcc atgccggcga tgcgcttgct gatcgcagac
     1141 gtggtggtgc cggcgcgccg cgccgcgctg ctgaaagact cggcttcgac ggcgcgcacg
     1201 aaccacgaga gcgcgccgag atcatccagc gcggccggcg ccgccccgac ccttcctgaa
     1261 acgcgttgcc gcgcaggcgc tcttcgcttc gagttcggca tttggcgcgt caggccaacg
     1321 cagagcgcac ttccatcaag gcaaagccga gcaagttgag cccccgccac tgctttggct
     1381 cgcgcgcgtg gggatggccc gcgtcgaggc cgatccccca aacacggtcc gctgggcttg
     1441 cctccacgag aacctggtcc tgcgttccgc gcaagaactc gagcaaggcc gcgtgctgac
     1501 cgaacttcgc gagatttccg cgcaccacga tgcgcatgcg gtgctcgcac cacgtgctct
     1561 cggcgaagtt tcggaccgaa cgcccgagcc gcttggcgtc cgccggatgc ggagccgcca
     1621 agatggcccg tacggactct gtatctgcga atagccgggc cttctccgcc atcatgtagt
     1681 gctccgccgt ggcgtaacga acgccgtcga tttcgaactc actaccgtac cattgggaaa
     1741 ggcagttttt gccaatccca tccgcggggc tcgcaccgtg cccccaaaag aaggtgtagt
     1801 cggcgacgcc gccagcttcg atccagtcga ccaatgcttc gcggcttcgg agatttcgag
     1861 gtgtcatggc gagaccgaga tcgttgagtt catcgagagg taccttgggc tcgcggcgcg
     1921 cgccagctcg acgcgcgcgc gttggcttct cgactcagtt cgtccgtcgg ttgttcgtcg
     1981 gtcgtccgtc gctcagtgac gcctccggcg gtacgccgcg gccagtccga gcgcgaggca
     2041 tagcccgtac accgtcgtgg caccggccga gttcgcctcg gaaccgggct tactcaaggc
     2101 acaagacacg gaaccttccg ccgaaccttg gcagcgccca ttggagcagc ggccttgcgc
     2161 actgccttgc accaccacgt cgatggcggc caaggcgtcg aggcagcggt ccaggtgccc
     2221 gccatggtcg atgtattgat tgttacaata caaagcgcca tcccctgaac tgctgcagtc
     2281 gatctcacaa cggcccttga gacgcgcttc acattgcacg aagcccgagg cctggcagtt
     2341 gacctggcag cgcgcgttgg cctgcgcacg acaagaccct tcacacgaag cctcgcattt
     2401 accctgacag gtagcgctgc caggctgacc ttggcagctc gcctcgcact gcgcggagaa
     2461 gctggctttg caggatgctt ggcaacgcga gcgattgctc gacgagctgc actgcgcgtt
     2521 gcattcggct tcaccatccg cttcacagcg cgcttggcag tcgagtcgcg gcgggttgac
     2581 ggcacactcg gcttggcagt cggcttcgca ttggccaacg cactgaacgc tcgcgctcgc
     2641 ggagcagctg ccctcacagc tcgcttcgag ctgcgccgcg caggcggcct gaaaactcgg
     2701 cgcggtgcat tcgccggtgc actcgatgcc agtgcgaagc tcgcaactcg aacgagcctc
     2761 gagatcgatg tatccgcact ccgcgagccc tgcgcgcgcc ggctgtgaca ccgacaacgt
     2821 caaaccgaag gccgtgagag aagcaaggaa gccgtaggta ctcttcatgt tcgttccgcg
     2881 tccggaaatc cggagaaacg gtccggagaa acaacttggg agaaacactc cgtcggttgt
     2941 acgccaccgg cagcgagttg cttcctaccc ctcgaagaaa gcgaattcgc ggtctaggag
     3001 acctgttccg aaacgatacg cgcgacgccc ggccccgaga ttccacgcac ccgtgaacgg
     3061 aatggctgag gcctcgaccg cgagccgagg cgcgtagccc gtagacccga gggcgagaac
     3121 cagccgacat caatgctcct tcgtgggggt gatcgaggaa tgtcgacgct gccgttcgtt
     3181 cagccatgac gaactagcgg accgcgatcg ccactttgcc gagcgtgtgt ccggtctcga
     3241 tttggcggtg accgtcggcc acgctctcga gcgcgaagcg gtcgctcacg tgtggcctta
     3301 gcaaccctcg atccaccaaa gacgcgattc cggcgaggat cgccccttgg cgctccgggt
     3361 gttcgccgta agccacgcgc gcgcccatga actcatagtg aacggtaatg ctgcgataga
     3421 gcagtgcgga ggcgcgcgcg cctggcttgg aatctagaat ggtgacgagc tgcccacagg
     3481 gagcgacgca ctcgatactg cgctcgaaga cctctcctcc gacggtatcg aacacgcacc
     3541 tcaaaccctt tccgtgggtc agctcgctca cgcgggccac gaaatcgttg ctcgagtagt
     3601 cgatcacctc atccgctccg agctcctccg agcagaaagc gatcgactcg ggacgccccg
     3661 cggtggtgag gactttgcag ccgtgcagcc gtgcgagttg gacggcgatg tgccccaccc
     3721 ctcccccgcc tgcatgcacc aggatcgtct cacccggctc gatgcgggcg cgttcgtgca
     3781 aagcttccca agcggtcaag ctgacgagcg ggagacaggc cgccgttgcg tgatccagag
     3841 agttgggctt cttggcggca gaccgcgcgt ccagcagaac gagctccgcg ttagcaccgt
     3901 tgccgaacaa gctgggacag ccgtacactt cgtcgcccgg cgcgaatccc actaccctgg
     3961 cgccgcaagc gacgaccgtc ccgctgacgt cgtagcccaa aatcagggga aacttgcggc
     4021 ccgcaccggc gcggcggacc tttgcgtcga cagggttgac cgacgtggcg tgaacttcga
     4081 ccagcaaatc gaactcgccg ggctccggcg cgggttgctc cgaaagcttg agaacctctg
     4141 ggcctccgtg ctccaacatg acgactgcgc gcatgccgtt gtcttactca tggacgcacc
     4201 tgaagaacaa gcaccgcgta ggctgattcg atgtggcgag ttggcgcgac tcgagcgctg
     4261 gcacgccctg cctcgcgggt tggctctaga cccatcgcca atgctgccgt ccgcttgaat
     4321 tcttcgcccg cccacgcaac cacaatcacg cgaggctcgc ccgcagagca actggtctat
     4381 agtgcggagc tgctgcgatg catacttcgg ccctgcgcgt cctgctcgtg gatgacgatg
     4441 aggaaacctt ggagttggtg ggcaaggcgc tgactcgcga gggacacgag gtgttgtgcg
     4501 cgcgaaccgc cgagcaggcc aatcagctac tcgcgcaggt tccgatcgag atcctggtgc
     4561 tcgatgtcat gctgcaacag ggctcagggc tcgagttgtg cgccgagctc cgtcagcgtg
     4621 ggaaccaagt tcctatcttg ttcctgagcg cacggggaac cgtcaacgca cgagtcgaag
     4681 gcttggaagt gggcggggac gactacttga ccaagccgtt cgcggtccgt gaggtcgtag
     4741 cgcgggttcg cgcgctcgga cgacgacgcc ctctgacgcg agacatgcac gtatccgtcg
     4801 gcagcctgac gttacatttc gccgagcgac gcgccgtcgc tcatgaccag caaatcccaa
     4861 tcacccgccg cgagtgggat atcttgaggg cgctggggaa cgcgcgtggc cgagtagtgg
     4921 cgttcgacga gctgctggag caagcctggg gcgaagtgac cgagaagggc cgggccagcc
     4981 tggaggtgat catgtctcgc ttacgccgaa agatcgacgc ggccgcgggc cgcccgttga
     5041 ttcgaacggt acggggtcac ggctatgcgc tggagctcga cgggtgaccc ggcgtcgctc
     5101 gatccagcag tctctgcgcc acacttatct gctctcgatt gcgacgatcg gggcgtcgct
     5161 ggtaggcctc ggagcactct cggcgaccta cgtcgcgttg ctacgggatg acgcgaattc
     5221 acgagccctc gcgcagctgc tcggtgggga gctgcgcgag cacgcgcaag actcgctctc
     5281 cgtccaacga gaggcgctcc gccgcgagct cgaggagcaa cgctggttcg agagagaaac
     5341 cgaggtctgg cgggagcgag ttctgatcgc cggcacccgt cccgaggccg agctggcgcg
     5401 ctggaccacg ctactcggct gcaccaccgc gcgactacac ggcgcgtggc accgcatttg
     5461 cggtgcgcgt ccggatccct ccgtggcggt ggttgtcgct tcccccgtgg gccccgtatt
     5521 tcgagcccag ctgccggtgg tggctgctat atcgatcgcc accgcgctgg cggtgctcgg
     5581 attttcactc ctgagccgcc tattggttca ccgatcactc gatccgctgg cccgcttcga
     5641 gagcgcgatg gcacggttgc ccgcaaagcc cggtcagcgc ctccaatccg aatggggcgc
     5701 gcgcgagatc aatcacctgg cgcaaacctc gaacgcgcta ttggcgcgcc tggatgaaat
     5761 cgtagaacga gagcaggcat tcgccgccaa cgccgcccat gaactccgct ctccgctgac
     5821 ccgcttgcgc gggcaactcg agctgttgtt ggacgagccc cacgctcgag caaccaaccc
     5881 aagcgcggca gagctgcgaa accgactcga gcgagcggca aagacttgta cggagctcgt
     5941 caactgcacg gaagccttgc tggccctcgc gcggcgcgaa gccagcctgc acgaggccgt
     6001 caattgggag gaagtcgcgc tctcgatttg cgacaaccta tccgatcagg agcgcgcccg
     6061 agtgagcgtg aacgcgcagg aggccctggt ccgcggcgac ctggccttgc tcggcctcgc
     6121 cgcgcaaaac ctggtcgata acgcgctgaa gtacagcgcc ggtccgatcg agctgaacgt
     6181 gctggccgcc gagaagcgct gcagcttcgt cgtggtcgac tgcggtccgg ggatttcgag
     6241 ctccgagttg gatcgggttc ggcaaccatt cgttcggggc agcagtaacg tccgcggaac
     6301 gggcctcggc ctggcgctcg tggagcatgt cgcgaagctc catggcgggg agctctcgct
     6361 aaagaatcgc agcgagcgat cggggctcat cgctgctctg agcctgccgc gctggtcccc
     6421 ctcgcgtccc taggcaaccc tccatcgcgc agggattgag actccacgct cgagtcgaat
     6481 catggaagat tctttcggaa tcgagtgatc ccttaacggc accttaacag cggttgccgc
     6541 ccgcatccga agggccattc ttcgctcgcg gtctccggcg ccgtcatgac tttcattcag
     6601 gatcgatgtc ctcccgagga cgggaaaagt ggccccagcc gcaagccgga agcccccatt
     6661 cgccggagca tggctcgcat cttactgatt gatccggatg agcaagcgcg tctcttggtc
     6721 ggttgcgcgc tgtccgcggc tggctacgag ctggtgagcg aaaccaccac cggggaggcg
     6781 ctggcctcgg tgcgccgcgc gccaccccag ctcgtgatca gcgagtgcgg cgcgccgggg
     6841 gtcgaattgg aggcgttgct gcagttcttt agcgatgccg gggtgctcac ggggttgcca
     6901 atcctggtgc tgagtgagcg cgcaagtgag gccgatcggg tcgccgcgtt cgaatcgggc
     6961 gtcgatgatt tcgtagcaaa accgttcagc cctcgcgagc tcgtgttgcg ggtccgcgcg
     7021 ctgctgcggc gctgcggaga cagcgccgaa acgggcgcga cgtggcagca cggggcgctg
     7081 tgcgtggacg tggatggtca ccgaacctgg gtcgaagggc gcgaggttca gctcacggcc
     7141 accgagttcc aaatcctggt tacgctgcgg acgaatcggg gccgcgtcat gagccgagag
     7201 cgcatcatgg ctgggttgtg gggcggcgcg cgcgtcggct cacgcacgat cgacgcccac
     7261 gtcaaacgcc tgcgcacgcg cctcggcgcc gcaagcagct gcgtcgagac ggtgcgtggg
     7321 gtcgggtatc ggttcgtcga gccctgaacc ggtcaatccg aggcttagcg cgggaagccg
     7381 ctgctcagcc gagcgaacgc gactacactg ccctccccca tgcgcgcttc gtccttgtgg
     7441 tcggtattct tgctggttgc ctgtcaaaaa gcagaggccc ccgagaaccc tgcgccggct
     7501 caaccggctc aaccggcacc gccagcgcac aaacaagtag cccttcagga tacgtcgttc
     7561 aaacgcccgt ccgccgaacg catcatcgcc atcggtgacc tgcacggcga catccgggcc
     7621 gcgcaggccg ccctgcgcct cgggggggca ttgggtccgg atgggcattg ggcgggcggc
     7681 aagttggtcg tcgtacaaac cggcgatcaa ctggaccgag ccgacgaaga gccggaaatc
     7741 ctcgagctcc tggatcgctt ggcggccgag gcacccgcgg ccggtggcgc gctctacacc
     7801 ttgaacggca accacgaggt catgaatgtt caaggcgact ttcggtacgt gaccgaggat
     7861 ggcttccggg acttcgccgc caccccgcta gacggtctcc acacccggga gattcgctcc
     7921 ttgcccagcg accaacaggg tcgggcggcc gcgtttctgt cgggcgaggt cgtggcgaag
     7981 cggctcgcac aacgcccggt cgtgatccaa gtgggcgaca gcgtgttcgt acacggtggt
     8041 ctactcgagg cgcacgtaga ttacgggctg ggtcgcatca atcgtgagac tgcacaatgg
     8101 atgcaagcgc ccgattcgaa accagcaccc gccatcttga ccgaggaacg cagcccaatt
     8161 tggctgcgcg cttactcgga tggaatccct ccagctggag tctgccaaga gctcgggcgc
     8221 gtgctcgatc ggctggaagc caaacggctg gtcatgggcc acaccgtgca gcgccaaggc
     8281 atcaacagcg tgtgccgcgg acgcgcgtgg cggatcgacg ttggcttgtc ccgcgcctat
     8341 ggccagaatc caccgagtgt gctcgagatc agcggggaca ccgtcaaggt actccaaggc
     8401 gaccttacgc tactcaccac tcccgccgcg ccgagcgcac cgatccccaa accatcgccg
     8461 agcaccaaag cgcccgttac caaaagctcg tccagcccga acaagcccaa agcccagccc
     8521 gccaagaact caccggctcc tgcctcgccc tgaggcagcg tgccgaatcg tgagcgctca
     8581 cgagcaatgc tcactccacc gtcacggtct tggcgagatt cctgggctga tccacgtccg
     8641 tgcccttcaa atcggcaatg caataggcca ggagctgcag cggaatcacc gtgagcagcg
     8701 gctgaatcac ctccggcgcc gcaggcaccg tcagcgtaaa atccgccaac ttgccgagcg
     8761 aggcatcccc ttcggtaatc accgcgatca cgcgaccgtc gcgcgcgcgc acctcttgta
     8821 cgttactgag catcttctcg tattgcgtat ctttgggagc cactaccacc accggcatgg
     8881 cttcgtcgat caacgcgatg gggccgtgct tcatttcgcc cgctgcatag ccctccgcgt
     8941 gcgcgtacga gatctctttg agcttgaggg ccccctcgag cgcgatcggg aaacccgcgc
     9001 cgcgccccag gaacaacatg tgtgaagcgt gttgccaacg tcgtgccacc tcttgaacgt
     9061 gcacgtgact ctccagcgcc gtacgcatca agcccggtgc ttcgaacagc gcctgcaaca
     9121 cctcgcgcgc gcgatctggc ggcaagctgc cgcgtcggcg accgaggtga accgcgatca
     9181 tcaacaacgc cgcgagctgg gtggtaaacg ctttcgtgga cgcgacgcca atctccggcc
     9241 ccgcgtgcgt gtacaacgtg ccctgcgccg cgcgagcgat ggcgctgtcc accacattca
     9301 ccaccgagat cacgctcgcg ccggcctcgc gggcagtgcg caacgcggcc agcgtgtctg
     9361 ccgtttcacc cgactggctg atcgcgatca ccaagtctgc aggcgtaaat accggatcgc
     9421 ggtagcgcac ttcgctcgcc agctcggcct gagccggcac gcgcgcatac ttctcgatcc
     9481 aatttcgacc tgccaacgcc gcgtgatagc tggtaccgca agccaacaga cacacgcgcg
     9541 agacgcgccg cgccagctcc gcgccaaacc cgatctccgc atccacgagc tcgccctcgg
     9601 cgatatcgat gcgcccgcgc agcgtgtccg agagcgcgct cggctgctcg aagatctcct
     9661 tgagcatgaa atgcttgtaa ccgccctttt ccgcctgaat cgggctccaa tcgatgcgct
     9721 tcggctcgcg ctgcacccgc tccccctcga gcgtctcgat gcgcacaccg tcgcgcgtga
     9781 gctctgccat ctccccgtcc tcgaggaaca gcatgcgccg ggtttgcgag agcagcgccg
     9841 ggatgtcgct gccgcacaat gtctcgccgt cccccaaccc gattaccaac ggcgatgcac
     9901 tcttcgccac tacgatcgaa tccgggcggc tcgcggacac gatcgccaag gcatacgcgc
     9961 cgcggatcct cgacaaggcc gcccgcaccg cgtcgaacaa cacggggtga cgggcgagct
    10021 cggcgtgcac cagatgggca acgatctccg tatccgtatc gctcgcgaac gccacccccg
    10081 agccctcgag ctccgccttg agctccaagt ggttctcgat gatgccgttg tgcaccaccg
    10141 ccacgtcacc ggctacgtgc ggatgtgcat tcggctccgt gggccgccca tgcgtcgccc
    10201 agcgagtatg cccgatgccg agcgtaccat tcagctgccg ggcgctggcc gcacgttcca
    10261 ggttatcgag cttgccgacc gcgcgtacca cgtctagccc gtgcgcggta tgcacggcca
    10321 aaccggcgga atcgtagcca cggtattcga gcttgcgtag gccgtccaaa aggatcggcg
    10381 tcacattgcg ggaaccaatg tagccaacga tgccgcacat gccgggcaac ctagcatgcg
    10441 accccaaaac aggccgcatt tgccgcatct tttgcgtccc gttcactagt tccaagctcg
    10501 ttctttgggc atctatcacg tgggcagctg tcgttcggga tctgccgcgc ctcacgcgaa
    10561 cgtgcaggag cgccgtgcgg ccaccccgca ctcgcgcagt cctacccggg tcttaccctc
    10621 acctgtgctg acctcagtcg tcagctcgac ccgggtcttg ggcatcacct ccccgcacat
    10681 tctgcagctc caggcgctga gacgcgggcc gcgcaccgcc gaattgcctg aacttccgcg
    10741 gattttcggc gctgcgcagg cagagtttga caacctgagg cgtcagatca aaagtccgcg
    10801 ccgtcatgtc cgcggccagc acagcgtcga aaactcgcta ccgatacccg atccacgccg
    10861 tcattctcgt agtggccgct tggctcttct tgctggccct gttggccggc tacgcgacgg
    10921 ttgccatcgc gctgttcccg atgtccccca tcgtcgccat tggcgccgcg tgcttgattg
    10981 ggtccgtgca cagctacgcg gtttccgtgc gcgaaaccgt tcgggactga gcgcactccc
    11041 cacgcgccag cccctgcagt ctcgcggggt tgcttgcgta ctcacttcta gcggccgcga
    11101 ctcgtcgatt cgagcgtttg gatcacggcc gcgatatccc gttcgccgag gccgagcgcg
    11161 cgcgcagttt ggaattgctc gcgaatcgcc gcgaggccgg gcattgcggt cggttgcgtc
    11221 gccgcctcct gcagcatcaa cgtcaggtcc ttcaccagca gatccacgga gaagtgggtc
    11281 tcgaagttcc gctctcggat cgcgttgccc ttgaacgcgt aatacgggga ggcgaaaccc
    11341 gacgctgcga tcacctccaa gatcttctct ggcgacaagc cacccttggt cgctaccgtc
    11401 atggcttcac aaagcccctc gagcatgaac gcgatcagcg tgttgcccgc gagcttcatc
    11461 aacgcgccct gcccgttctc cccgcagtga atcacgcggc tgcccatctt cagcagtaac
    11521 ggttccaatt cgctgttcag ttccgccggg cctccgctca tgaacagcaa cgtgccgttc
    11581 tcggctgcat tcttggaccc cgtcaccggc gcttccaaaa acccagcgcc gcgctccgag
    11641 aacgccttcg ccaggcgccg cacgagctcc ggggagaccg tactgcactc cacgtaacgc
    11701 aggcctgcgc gcgcgcccgc cagcacacct tccgcgccga acaccacctg ttcgaccgcc
    11761 tgcggattcg aaacacagca cacgatcacg tccacgtcct gggccaattc gcgcggcgaa
    11821 cgtgcgaccg gcacctccac gccgagcccc gccgcgcgtt ccggtgatcg attccagacc
    11881 gttaccggaa cgcccttctt gctcagattc cgcaccatcc ccgaacccat gatgcccagc
    11941 ccgatgaatc caactcgttg catgcatacc tccaagcctg cttttcaacc tgaatttcgc
    12001 gcccacgacg gacgaggcag tgccggcttc tgactatctg agcgtgatag cagcaaagct
    12061 tctcgccgta ctacaacgtg cgcgaagcgg cccagagccc gcccaaagcg cagccgctcc
    12121 cctctcagac gcccgctcca ccacgccggc gccccccata accccgacga agaccggaga
    12181 accgcaggtc gcaaagcacg cttcattgcg agttactcgg acgtcggact cggccccaac
    12241 ggatcccccc gatcgacggg gcgcctcccc ctcgggcgcc ccggattcgc aggagtcttc
    12301 tgctccatgt cgaactcacg ctccgtcgcg gcgggttgaa agctgcggac ccaagttggg
    12361 gccgccggag gcttggagta catgagctga gaagtccatt cctcgtcggt catccgatgc
    12421 gagctctcgg tcgtgaactc gtaataggaa tatacgggcc caacatagac tccgcggtgg
    12481 ctctcgttgt cgatcgccac cgccaagtag ttcgcacgac cggtgcccac ctcgagcacc
    12541 tggttcgagt ccggatccgt atgtacatcg gcgatgacgg gcttgaacgc cagcggtgaa
    12601 gacccgtaca gaagcgacgc ataccagccg tggaacgtgg gagagccgca acccaccatc
    12661 tccacctcga tcgtgttctt gagaaaccgt ttctcgtcgc tggagaaagg ctcggccgcg
    12721 agctctttcc gagccatggt ctgcaagcgt gccaaggttt catcgaagcg ctgcagcagg
    12781 tccccccagt gcttgcggag cgaaacttca ccctcttcct tggaatacgg cagggccgct
    12841 agccgcaaag aaagccctcg catcacttca cccagccgtt gatagaactc gggatatggc
    12901 tctacgtaac cggtcgggta ctcacacagc acggagccag tataagactg cttgccgtac
    12961 aagatattgt cgtggcgcaa ctcagcccac gatcccagct gcgtttgca
//
LOCUS       DASTKU010000929         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1073888, whole genome shotgun sequence.
ACCESSION   DASTKU010000929 DASTKU010000000
VERSION     DASTKU010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073888"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFQ61_32850"
     CDS             complement(<1..502)
                     /locus_tag="VFQ61_32850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959341.1"
                     /translation="MTPEPGLPTPKQAVLAWVFGLVLVALIGLLVAIAAMMVAVQQGL
                     GWDRALSLVMDPAGSPLVTSPSWIALGIAANELAVAALLLRWKRRLRAPLAAIVPLTR
                     PSGMALLGSVLLPFGLSPLAEVFGEFVRRALPLEITSDHLVTAVSRNTSPLLFGGILF
                     AAAVLPA"
     gene            665..1390
                     /locus_tag="VFQ61_32855"
     CDS             665..1390
                     /locus_tag="VFQ61_32855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959342.1"
                     /translation="MGSIACDDAPSPRAKLDSAPAKSERPPEPTAIKAPAPSGPPELV
                     IDTISPKVGFERALFQYPDGRQKLADALGQSKGKFGQDVHLAVERKANPAWVAAYLNE
                     LSKIDVAKVDVHTETRSEFSSQVVFTPEIRAGKPAPCSPVVMILSDRSTAVWKLGGGV
                     AGRRPKGMAGPDLSMTAETIERVAKSCKGSASLFVASAPEVEWGLVFDLAASARSLLP
                     GRFDSIVFVSTTPVPGHAIEFGR"
     gene            complement(1410..1991)
                     /locus_tag="VFQ61_32860"
     CDS             complement(1410..1991)
                     /locus_tag="VFQ61_32860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959343.1"
                     /translation="MILVRSMLAALDRGAHGAGLTVLPFYECAALVHALRLGKELALT
                     AKKSGGAVSQRLYVRTPSTLLPRSTAITLLGWWFAAACACTSCGAGRVDAPATLGTPR
                     RPIYKPGQSLEARLCECRECLDVGCCDGEPDSETEEPTADGSAPATETGEVTLGVSMR
                     VCGRCVRRTWTVRGLAACEPSAPRECCSNTVRG"
     gene            complement(2032..>2450)
                     /locus_tag="VFQ61_32865"
     CDS             complement(2032..>2450)
                     /locus_tag="VFQ61_32865"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959344.1"
                     /translation="IDERVDLYALGAVGYFLLCGEPVFTGDTTVEVCSHHLHTRPQPP
                     SERLDKTISDSLQRVILSCLEKDPKERPANAQTLVALLDGCDGVPPWGQEQAEAWWAS
                     RSALLARQVAPHVNSEPASDKQLAGASTAPLAKIPS"
BASE COUNT          484 a          777 c          769 g          420 t
ORIGIN      
        1 aggctgggag caccgccgcc gcgaacaaga tacccccgaa cagcagcggg ctagtgttgc
       61 gcgacaccgc ggtcacgagg tggtcggagg tgatctccag cggcagcgcg cgacgcacga
      121 attcaccgaa gacctctgcg agcggggaca gaccaaacgg tagcaacacg ctacccaaca
      181 gcgccatccc cgagggccgc gtaagcggaa cgattgccgc gagtggcgcg cggagccggc
      241 gcttccagcg caaaagtagc gcagcgacgg ccagctcgtt ggcagcgatg ccgagggcaa
      301 tccagctggg gctggtcacc aggggcgaac ccgctgggtc catgaccaaa gacagggcgc
      361 ggtcccaacc caagccctgt tggacagcca ccatcattgc ggcgattgcc accaacaaac
      421 cgatcaaagc caccaagact aggccgaata cccaagccaa caccgcctgc ttgggggtag
      481 gaaggcctgg ctctggtgtc accacgctgg cacgctagca cgcgattcgg tagaccttgc
      541 aagcgtgctt cccgagcttc cacctaggac cattcgaacg cgcctccgcg acttccgcct
      601 ccgcgacttg gcgccgaggc actccttatc attggggtgc tccttattat atggactcgt
      661 ggcggtgggc tcgatcgcct gcgatgacgc gccgagccca cgagctaagc tggactccgc
      721 tccagcgaag agcgagcgac ccccggaacc cacggccatc aaggctccag cgccgagtgg
      781 acctcccgag ttggtgatcg acaccatcag tccgaaggtg ggcttcgagc gggccctgtt
      841 tcaatatccc gacggccgcc agaagctcgc cgacgcgctc ggccaaagta agggtaagtt
      901 cgggcaagac gttcaccttg cggtggagcg aaaggcgaac ccggcttggg ttgctgctta
      961 tctgaacgaa ctctccaaaa tcgatgtcgc caaggtcgac gtccacacgg agacgcgcag
     1021 cgagttttcc tcacaggtcg tgttcacgcc ggagattcgg gctggaaagc cagcaccgtg
     1081 tagcccggtc gtgatgatct tgagcgatcg aagcactgcg gtttggaagc tcgggggtgg
     1141 cgtggcgggg cgcaggccca aaggtatggc tgggccagat ctcagcatga ccgccgagac
     1201 catcgagcgg gtagcgaagt cttgcaaagg ctctgcatct ttgttcgtgg caagcgcgcc
     1261 ggaggtggaa tggggcctcg tgttcgactt ggccgcctcg gcgcggagcc tcttgccagg
     1321 ccgcttcgat tcgatcgtct tcgtctcgac gacgcccgta cctggacatg ccatcgagtt
     1381 cgggcgttga ctgagtcgta ccttcgagat tagccgcgca cggtgttcga gcagcactca
     1441 cggggcgcgg aaggctcgca agccgcaagc ccccttacgg tccaggtgcg ccgcacgcat
     1501 cgtccgcaca cgcgcatgga cacaccgagc gtaacctcgc cagtttcggt ggcgggggcg
     1561 ctgccatccg cggtgggctc ttccgtctcg gagtcgggct cgccatcaca gcacccgaca
     1621 tcgaggcact cacggcattc acagagtcgc gcctccaagc tttgaccagg cttatatata
     1681 ggtcggcgag gcgtcccaag cgtggcgggg gcatcgacgc gacccgcccc gcagctcgta
     1741 catgcgcaag ccgctgcgaa ccaccaaccc aggagggtta tcgcggtcga tcgaggcaaa
     1801 agggtactcg gtgtgcgcac gtacagacgt tgggacactg ctccgccact cttctttgct
     1861 gtaagcgcca gctctttgcc gaggcgaagt gcatgaacta acgccgcgca ctcgtaaaag
     1921 ggcaggacgg tcaggccagc accgtgcgcg cctcggtcca acgccgcgag catcgatctg
     1981 accaggatca tccgttctcg ggagcttcgg atacgatcga aggagcggat cttaggaagg
     2041 gatcttggct aacggtgcgg tagaggcacc cgccaactgt ttgtcggacg cgggctccga
     2101 gttgacgtgc ggcgccactt gccgagctag aagcgcgctt cgcgaagccc accaagcttc
     2161 ggcttgctct tggccccatg gcggaacacc gtcgcatccg tccaggagtg cgacgagggt
     2221 ttgtgcgttt gcgggacgtt ccttggggtc cttctccaag caactcaaga tgacgcgctg
     2281 aagcgagtcc gagattgtct tgtccaagcg ctctgatggc ggctgcggtc gagtatgcag
     2341 gtgatgactg catacctcga ctgtcgtatc gcccgtgaac acgggctccc cgcacagcaa
     2401 aaaatagcca accgccccga gcgcgtacaa atcgacccgc tcatctattt
//
LOCUS       DASTKU010000930        18437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_181142, whole genome shotgun sequence.
ACCESSION   DASTKU010000930 DASTKU010000000
VERSION     DASTKU010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 18437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18437
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181142"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFQ61_32870"
     CDS             complement(<1..191)
                     /locus_tag="VFQ61_32870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959345.1"
                     /translation="MFILALKLFTVPLLILLVSLAGQKFGPRLAGVLTGLPVIAGPIA
                     WLLGLEHGVEFAARAAVGTL"
     gene            complement(291..1064)
                     /locus_tag="VFQ61_32875"
     CDS             complement(291..1064)
                     /locus_tag="VFQ61_32875"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9959346.1"
                     /translation="MSGAISLSGRVAVVVGGTSGIGKAIALGLARAGADVVATGRRES
                     ELAQVAQEIEATGRRTLRQTSDVTDASTLEALREGVLQSFGKLDILVNAAGKTKRTPT
                     LDLPLPEWQDILDTNLTGTLRACQVFGRPMLEKGYGRIINIASLSSFVGFYEVAAYAA
                     SKAAVASLTKSLAVEWGKRGVLVNAIAPGVFVTSLNAALLEGTGRGRELLARTPLGRF
                     GAPEEVAPAAVFLASDAASFVNGEVLAVDGGFLASGVNQ"
     gene            complement(1111..1761)
                     /locus_tag="VFQ61_32880"
     CDS             complement(1111..1761)
                     /locus_tag="VFQ61_32880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET9959347.1"
                     /translation="MTQTPSVLRRIEEAFIIPVIRAPSESLAQRAAEAILQGGIGVVE
                     ITMTVPGALDVIAGLRARLGDKVLLGAGTVCSARDAERCIDAGAAFLVSPGWDAETLH
                     FAQARGVVHVPGVLTPTEVMQASKGGAQIVKVFPCSAVGGAKYLKALRGPFPEMRFMA
                     TGGINLQTLDEYVKAGASAVGIGGELVDLTLLSAGNDAAITARAAELVAALAKSRG"
     gene            complement(1758..2855)
                     /locus_tag="VFQ61_32885"
     CDS             complement(1758..2855)
                     /locus_tag="VFQ61_32885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET9959348.1"
                     /translation="MQTLALLPASQCRFDAVSLGEVMLRLDPGEGRIHTTRTFQAWEG
                     GGEYNVARGLRRCFGLRTAVITALADNPVGRLIEDLMLQGGVDLSYLRWVDYDGVGRR
                     VRNGLNFTERGFGPRAAQGCSDRGHTAAAAMQAADYDFEPLFSRDGVRILHTGGIYCA
                     LSESTPEVAAKAMQSARRHGTRVSYDLNYRPSLWKSIGGKRRAQEVNRSLMPYVDVLF
                     GNEEDFSAALGFDVEGLDLAAAELGTTAFKKMIETVIKAYPNIQTIATTLRTARSATR
                     NGWGAIAYHAGEFFEVPQQETEILDRVGGGDSFASGFLFGLLNGRGCEYAVRCGVAHG
                     ALAMSTPGDTSMASLAEVEQRMNSSSARIER"
     gene            3167..4372
                     /gene="manD"
                     /locus_tag="VFQ61_32890"
     CDS             3167..4372
                     /gene="manD"
                     /locus_tag="VFQ61_32890"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF011654.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HET9959349.1"
                     /translation="MKIVDAEVIVCCPGRNFVTLKLTTEDGVYGLGDATLNGRELAVA
                     SYLRDHLIPLLIGRDARRIEDTWHYLYKGAYWRRGPVTMTAIAAVDMALWDILGKALN
                     TPVYQLLGGAAREYVTVYGHAFGESIEDAVASVAEHMQAGYRAVRAQCGIPGLPKNYG
                     VGQGKFYEPAEKGKATETVFSAERYLDVVPRLFERIRRELGPEVQLLHDVHHRLTPIE
                     AARLGKQLEPYHLFWMEDPVPAEHQESYRIIRQHTTTPIAIGEVFSSIHDCQVLIQEQ
                     LIDFIRSTVVHAGGLTHLRRIAHLAELYHVRTGCHGATDLSPVCMAAALHFGLAVHNF
                     GIQEHMPHNPETDRVFPHLYKYESGGMHPGQAPGLGVDIDLELARTYPYDPAYLPVCR
                     KLDGTLHSW"
     gene            complement(4508..5761)
                     /locus_tag="VFQ61_32895"
     CDS             complement(4508..5761)
                     /locus_tag="VFQ61_32895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9959350.1"
                     /translation="MSEIRVLFVQSQAFFGSDSMIHSLLMQYLDRGRVEVHVACNQGK
                     AGEKSASLKALEKIEGIKIRRTEFGPSIHGESVSEAARLLWESGPSGLASLAGLVSYA
                     KKNRIQIVHGTEKPRDSFYGHLLARAIGARSVTHVHVKVEDWISPLVRWAMKHNDGLI
                     GVSDFVAKSAVAAGYDAAKTHFVLNSIDARRWNPDLDGSEVRREFGIAPGTPVLSIIS
                     RLFPWKGHSDLLRALQIVKAQNPNFRLLVVGEDDPRATPGGGSYLAQIKSLSAELGLS
                     DNVTFTGFRSDIANLLAGTDIYTMPTFEEPCAVAFLEAMAMAKPVIALESGGTPQLVE
                     HGKSGLLSLPKDNEALAKNILSLLSDPERRLEMGRYARQRVEQYYNPQRMADEVEGVY
                     RRMLHLPQPVARPPARVVGEASSSL"
     gene            complement(6094..6273)
                     /locus_tag="VFQ61_32900"
     CDS             complement(6094..6273)
                     /locus_tag="VFQ61_32900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959351.1"
                     /translation="MSASAKLVLLSILIMSIVIPARAAMTKDPERGLRKALKQMAIFN
                     LLYVLAVTFLYPRLL"
     gene            complement(6383..7948)
                     /locus_tag="VFQ61_32905"
     CDS             complement(6383..7948)
                     /locus_tag="VFQ61_32905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959352.1"
                     /translation="MASSRLGASEKAAVAIAVVLTLVAVAIDNAAVGWLLGVPITLLL
                     FWFATRVPVIYSLVGVMFCAFSLENPSEATASGQWRSPLYVVGALLLAHMNQVTGVSF
                     MSISGTDMFLIFLIGVVLQRRASKSKIDGGWAPTPKPLIDLAKLSLAGAMFVWGRGLI
                     AGGDFSKSLWQIEKVTYLPITFLLFQYAVRGPKDYLLLGRTVITAALVKAAVAVYVMH
                     FADVGPEKPPWATSHHDSMLFACAVVILVAVLIHKAHPRAGRLALTIFPPICWGMIEN
                     NRRMVWVQIALVFLTLFFSMPPTRAKKKIVNALLYASPFIALYIAAGWGSPTGIFKPV
                     GTIRSVVEPANDVSTMTRDIENYDLAMTIRSNPLFGLGYGHQYLQIIGLPPMPHPLEP
                     WLPHNSLLGLWFASGFVGYTMITLLWTGGVYFGVRAYRALSNPMDRAAALVSFGAVLI
                     YMIQCYGDLGLGAWTGVYIVAPSIAVAGKLAVISGAWPMKGRHAKGKLETATPPQAAP
                     PPAGPGGPSGYAA"
     gene            complement(8160..8381)
                     /locus_tag="VFQ61_32910"
     CDS             complement(8160..8381)
                     /locus_tag="VFQ61_32910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959353.1"
                     /translation="MASPSRRVTARRSFERYGLSLGVRSRRGAGAVALGDLTDEDTES
                     IPATRSALVTLCARFPGGAASASRLSVLA"
     gene            complement(8556..10046)
                     /locus_tag="VFQ61_32915"
     CDS             complement(8556..10046)
                     /locus_tag="VFQ61_32915"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HET9959354.1"
                     /translation="MRSNESVSGEGSSFDGPRRGLGLGFTPALFAGVALVLGACSQNV
                     RGACVTCDPGALPSASPSSTATASPSGAPSTMPPPVMDDPMARLKTLLLPKDAKAPVD
                     VTNRYADDPRAAALGKKFFYETRFSGPLLDQANNGTSGTLGMQGETGKVGCVSCHVTD
                     GGAFADLRSPRQQLSLGSGWTHRKAPSILDVAQGQFFMWDGRRDSVFSQVFSPIESPL
                     EFNSSRLFVAQQVARLYRAEYEAVFGAMPDLSKYPALDASQAGCSELPSDPVSGQCGK
                     PGDQDEPVTRVVANLGKAVQAFTRTLSCGRSRFDEWLEGTETALSDQEKAGALLFVGK
                     AGCSMCHSGPYLTDGRFHNVGLSGGLVPFTGVATFNDPGAAPAVAVLLKDPLNSRGVF
                     SDGDDRRLDKLPADPQALLGSFKTPGLRCVSRRGSFMHNAEFRSLGDVVDFFNEGASS
                     GGFVGVAENAPRNLSREEREQLVAFLTALDGSGPDPAAMVPPTLAP"
     gene            complement(10036..11637)
                     /locus_tag="VFQ61_32920"
     CDS             complement(10036..11637)
                     /locus_tag="VFQ61_32920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959355.1"
                     /translation="MGIRVTSVANWATLIVCALSAGCSGQQQGPEAPTAGTAGLPAGE
                     GGLSAGGNVSSGGSLSGGAAAGGSAGANVAGSSSAGANNTSGAANGGSNAAGGATSPG
                     GSTNVAGAGPVQASGPCGLENPAFCEDFEGADKPGGRGGPLDENVWSVSRYGIASTIA
                     IFTRLAATSTESGVENTPTLCGQQFSNLLPPNDIRLCASEGGSRRLEEVMNDAGAFGF
                     TSMRIRQPFDFTGRVGTVAFEVDAKRNKDYDGHGWWTEIWISKEPVPMPYHGAPTVAS
                     YVTDAVGFQIAPAGGSCFGDVGCNQVGRVIVSKDYKLTRDTVISDSGGFKVADGKGNR
                     FKLLISQDSAEFWASDFDNPNTLRRVAVVKELGLRFSVGYVHIQHAQYNASKANASSS
                     QTFRWDNIGFDGPKQAKPRAYEIDDDGYSAKPQEHQVGYSLSNPVSVSVKDVDLSAAK
                     RAIFGFNLHAAGNKAFRYRFNGKEWHTFMTPSEFDSQITLRSFSVEVPLSELVAGVNK
                     LEMDQPQQDKPIEYVGNMDLTIDAQ"
     gene            13191..14333
                     /locus_tag="VFQ61_32925"
     CDS             13191..14333
                     /locus_tag="VFQ61_32925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET9959356.1"
                     /translation="MSPTTAAGGGAERVLLDHQRFLDRSRFEPVIAVLGQGTFAEEAK
                     SLGIEVYVAGPHSDRQPLATARELYNFGRFVRDRRIDVLVGNKYRSIFYWGLSQARRR
                     PFVWLLHDPLPQAGLHRKVLATVTDQLRPSHTVWVTPESSESYGQRFPHLLQSSSQIR
                     PGTCPEDLIEGADAQRARRRWGIPVDAPIAAMFARMQESKGHLDLVHAAATVLKEYPE
                     TRFLLCGGTLPNMDPSHETNVKRAIAELGVSDRVLTLGIISEEEKKDILAASTLLVHP
                     AVWEPFGIAVIEGMAVGKPVVVADARGPGLIVEHGQTGLVVPRSQPAALAEGISRLLR
                     DPELAREMGERGAERVRERYHARVATKRLEDIFDGLPPLAASAGAG"
     gene            14343..15899
                     /locus_tag="VFQ61_32930"
     CDS             14343..15899
                     /locus_tag="VFQ61_32930"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HET9959357.1"
                     /translation="MSASNAEAAEPQQPAPPTVPPNPEPQEPGHEQRKATGSLVLRNT
                     LFLMGAQIVNTPLSILINVMMGRYLGPNEFGLLYLASTFCSFGFLFVDWGQSSVVPAI
                     VARERSSVNGVLGSSLLFRLAAACIVYVFMAPLALIIAPGHGFQLTLVIVAALALVGS
                     LYSAFLDTLRGLERLDLSAYNGVGQQLLTGALVIPILLLGGRLHSVLLAQIAAVVVVL
                     AVAVKILGGLGVRPARPHMSHIKRLLHEGTPFVFFGFAMALQPNVDAGFLSRMVPTEV
                     IGWHAAARRLLGFLVTPAGALIGALYPTLCRLHAEDRESFVNVTRQGVQSALLLATPI
                     ALGCALYADIGVAIYSKEAFGPAAMNLRLLAPFILLVYVSMPLGSSLLAAGRQRTWAG
                     VQLVCVGVSLILDPLLVPLMQRRYANGGLGVCIATVASELVMVVCGVWMTPRGIFDRS
                     WLRKLLPLLAGAAGMTATALALAAVSLTPYVAAPLAVAAYGGTIWATGGVDPVYVEKF
                     KAKLRSKLKR"
     gene            complement(15896..17653)
                     /gene="treZ"
                     /locus_tag="VFQ61_32935"
     CDS             complement(15896..17653)
                     /gene="treZ"
                     /locus_tag="VFQ61_32935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HET9959358.1"
                     /translation="MSTVWVWAPNTEKVELVTGESRIEMKRIDGDRFCGPDLPPGTDY
                     QFSLNGGPPLPDPRSRWQPRGVHGPSRILDTQFEFTDSGFRAPPLASGVVYELHVGTF
                     AGDGTFDGVIPRLPHLRSLGVTHVELMPIAEFPGTRGWGYDGVYPFAAHSGYGGPLAL
                     KRLVNACHAQGMGILLDVVFNHFGPDGNYLPRFGPYLVDTFHTPWGAAVNMDGPRSDH
                     VRRFFIDCALQWMSEFRFDGLRLDAVHSIFDRSARHFLKQLAEETLELSNREQRPLIL
                     IAESDLNDPRMVHSSDAGGYGLDAQWSDDFHHALHVTLTGERNGIYGDFFGIRDLAKA
                     LERGFVVDGRYSAFRQRCHGAPFENLSLRRLVVAMQNHDQVGNRARGERITHLISPRR
                     ALLGAAVTLLGPSVPMLFQGEEWGARSPFQYFTEHHNPELARAVSEGRRREFAEFGWP
                     AEAVPDPQAPETFEASRLNFRELELPEHAAILEQYRALVALRRSRPELSSACVHAYAD
                     ERRGTLLIDRERSRVAVNFGSEAVRIPAGPLPNKPAPNKVGESDRGFEVIYGMGAPSI
                     EGQELVLPPESMALLVLSA"
     gene            complement(18070..>18437)
                     /locus_tag="VFQ61_32940"
     CDS             complement(18070..>18437)
                     /locus_tag="VFQ61_32940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959359.1"
                     /translation="SRPLRSSQRWRGVFPREVSGLSPQNHVREARPTSTLAQSYCPYF
                     TLETAPAPSAGLHSEATRKQSRPADFAAESARAARASTRSSALKPRVLSQAGFTIHSS
                     SPRSYCCTRVKSRRERRGG"
BASE COUNT         3453 a         5979 c         5647 g         3358 t
ORIGIN      
        1 agagtcccaa cggcggcgcg agccgcgaac tcgacaccgt gttcgagccc gagcagccac
       61 gcgatgggtc cagcaatcac cggcaacccg gtcaacaccc cggccaagcg aggaccaaat
      121 ttctggcccg ccaagctcac gagcaagatg aggagaggaa cggtgaagag cttgagggct
      181 agaatgaaca ctcgagctca cgtgcttttc caggcgctcc catcggttcc gcggcaaaat
      241 ccgacgagtt cacgcgcgag aagtcgatcg ccgggcggag gcagagaacg tcattgattg
      301 acgccgctcg ccaagaagcc gccatcgacc gccagcactt ccccattcac gaagctggct
      361 gcatcggagg ccaaaaacac cgcggccggc gcgacctctt cgggcgcgcc gaatcgaccc
      421 agtggcgtgc gggcgagcaa ctcacggcca cgcccggtcc cctcgagcag ggccgcgttg
      481 agcgaagtga cgaagacccc cggcgcgatg gcgttcacga gcaccccgcg tttgccccac
      541 tccaccgcga gtgacttggt gagcgaagcg acggcggctt tactcgcggc gtaggcggcc
      601 acctcgtaga agccaacgaa cgagctcagc gacgcgatgt tgatgatgcg cccgtagccc
      661 ttttcgagca tcgggcgccc gaacacctga cacgcgcgca acgtgccggt caggttggtg
      721 tccaggatgt cctgccattc gggcaacggc aggtccaagg tcggggtgcg cttcgtcttt
      781 cccgcggcat tcaccaggat atcgagctta ccgaatgact ggagcacgcc ttctctcagc
      841 gcctccagcg tggaggcgtc cgtcacgtcg gacgtctgtc gtaacgtgcg acgtccggtc
      901 gcctcgatct cttgcgccac ttgggcgagt tcgctctcgc gtcgcccggt agcgactacg
      961 tccgcgccag cgcgagccaa gcccaaggcg atggcttttc cgattccgga ggttccaccg
     1021 acgacgaccg caacgcgacc cgataggcta atggcacccg acatgcgcgc ggaacatacc
     1081 gcaaaacatc agcgcgagat agcggcggaa ctagccgcgt gacttcgcga gcgcggctac
     1141 cagctccgcg gcacgcgctg tgatggccgc atcattgccg gcagagagca gcgtcaaatc
     1201 cacgagctca cctccgatcc cgaccgcgct ggcgcccgcc ttcacgtact catctagagt
     1261 ttgcaggttg atgccgccgg tagccataaa gcgcatctcc gggaacggac cgcgcaaagc
     1321 cttcaagtat ttggctccgc cgacggcgga gcacgggaac actttgacga tctgagcgcc
     1381 ccccttggag gcttgcatca cctcggtggg cgtcaacacg cccggcacgt gcaccacgcc
     1441 gcgtgcctgt gcgaaatgga gtgtctccgc gtcccagccc gggctcacca gaaaggccgc
     1501 gcccgcgtcg atacagcgct cggcgtcgcg cgccgaacac accgtacccg cgccgagcaa
     1561 caccttgtcg ccgagccgcg cccgcaaacc cgcaatcacg tccagggccc ccggtacggt
     1621 catggtgatc tccaccacac cgatgccgcc ctgaaggatc gcctctgccg cgcgctgggc
     1681 gaggctctcc gaaggcgccc gaatcaccgg gatgataaat gcctcttcga ttcgacgtaa
     1741 tacggacggg gtttgggtca tcgctcgatc ctggcgctgc tgctattcat ccgttgttcc
     1801 acctcggcga ggctcgccat gctggtatca cccggcgtgg acatcgccaa ggctccgtgg
     1861 gccacgccgc agcgcacggc gtactcacaa cctctgccgt tcagcagccc aaacaagaac
     1921 cccgacgcga aagagtcccc tccgcccacg cggtccagga tctccgtctc ctgctgcggc
     1981 acttcgaaga actcgccggc atgatacgca attgcgcccc agccattgcg agtggccgag
     2041 cgagcggtgc gcagcgtcgt cgcgatggtc tgaatgttgg ggtaagcctt gatgacggtc
     2101 tcgatcatct tcttgaaggc cgtcgtcccg agctcggctg ccgccaagtc caagccttcg
     2161 acgtcgaaac ccagcgccgc cgaaaaatcc tcttcgttac cgaagagcac gtccacgtat
     2221 ggcatcaacg agcggttcac ctcctgtgcg cgccgcttac ctccgatact cttccacagc
     2281 gacgggcggt aattcaggtc atacgagact cgcgtgccgt gacgtcgcgc gctctgcatt
     2341 gctttagcgg ccacctcggg cgtgctctcc gagagcgcgc agtagatgcc gccggtgtgc
     2401 agaatgcgca cgccatcgcg tgaaaacagc ggctcgaagt cgtagtcagc ggcctgcata
     2461 gccgcggccg ccgtgtgccc gcgatccgaa cagccttggg ctgcgcgcgg cccgaaccct
     2521 cgctccgtaa agttcagccc attgcgaacg cgacgaccca cgccatcgta atcgacccaa
     2581 cgcaagtacg agagatccac gccaccttgc agcattaggt cctcgatcag tcgaccgacg
     2641 ggattgtcgg cgagcgcggt gattacggcc gtgcgtaagc cgaagcagcg cctcagcccg
     2701 cgcgccacgt tgtattcgcc gccgccttcc caggcctgga aagtgcgcgt ggtgtggatc
     2761 cggccttcac cgggatccag gcgcaacatg acttcgccca ggctgactgc gtcgaaacga
     2821 cactgagatg caggaaggag agctagggtt tgcatgatgc cggctcgttg aacgttgtat
     2881 ttggaaatcg acggcgtgag ttgggctcga gcacgcgtgg cgcgcggcgc cgcgcagcct
     2941 gaaacgctgc ccgaaagcta ctcgaagctc gacgctcttg tcatccatca gcgcgcgacg
     3001 ccgccgaaca gaacggctgc ttccggtagc tcgcgccaga agcgagaggc gcctcgccgc
     3061 cgcattttgg tggtaggcgg gggccggcac cgcggatatg gtgcccgacc agcagcgcgg
     3121 cgtttcattt ctttccaggt catggccgag ctcaggagac cgcgacgtga agattgtcga
     3181 tgcagaggtg atcgtatgtt gtccgggtcg caacttcgtc acgttgaagc tgacgactga
     3241 agatggcgtc tacgggctgg gcgacgcaac cttgaatggc cgggagctcg ccgtcgcgag
     3301 ctatctgcgt gaccatctca ttccactact gatcggccga gacgcgcgcc gcatcgaaga
     3361 cacctggcac tacttgtaca agggggctta ctggcgccgt ggccccgtga ccatgaccgc
     3421 gatcgccgcc gtcgacatgg ccctctggga catcctcggc aaggccttga atacgccggt
     3481 gtaccagcta ctcggcggcg ctgcgcgcga gtacgtcacc gtgtacggcc acgccttcgg
     3541 ggagagtatc gaagacgccg tggcttcggt ggccgaacac atgcaggccg gttaccgcgc
     3601 cgtacgcgcg caatgtggaa tacctgggtt gccgaagaac tatggtgtag gccaaggcaa
     3661 attctacgaa cccgccgaga agggcaaggc caccgagacg gtgttctcgg cggagcgcta
     3721 tttggacgtc gtgcctcgct tgttcgagcg cattcgccgc gaactcggcc ccgaggttca
     3781 gctcctgcac gacgtgcatc accggctcac gcccatcgaa gcggcgcgct tgggcaagca
     3841 gctcgagccc taccatctat tctggatgga ggatccagtg cccgccgagc accaagagag
     3901 ctaccgcatc attcgacaac acacgacgac gccgatcgcg attggagaag tcttctcatc
     3961 cattcatgac tgtcaggtgt tgattcagga gcagctgatc gacttcattc gctccaccgt
     4021 ggtgcacgcg ggcgggctca cgcacctgcg gcgcatcgca cacctggcgg agctctatca
     4081 cgtgcgcacc ggttgccatg gagcgacgga tctcagcccc gtgtgcatgg cggcggcgct
     4141 ccatttcggg ctcgccgtgc acaacttcgg cattcaggag catatgccgc acaacccgga
     4201 gacggaccga gtgttcccgc acctctacaa gtacgaatcg ggcggcatgc acccggggca
     4261 agcgccgggc ctaggcgtcg acatcgatct cgaactcgcc cggacttacc cttacgatcc
     4321 ggcttatttg cccgtgtgcc gcaagctcga cgggacgcta catagttggt gagtggcgcg
     4381 tacgagcgcg cttgcccgaa gtgcgctccg cacaaaaata gagctcgatg tcgcgaagcc
     4441 tcgctgagtg aggcttcgcg agcgggacga cggtgcgtag ctcacagcga gcagcgggcg
     4501 ccgtagctca cagcgagctg ctcgcctcac cgacgacgcg cgcgggcggg cgagcgacgg
     4561 gctgcggcaa gtgcagcatg cgccgataga cgccctccac ctcatccgcc atgcgttggg
     4621 ggttgtaata ctgctcgacg cgttgacgag cgtaacgccc catctccaag cgccgctccg
     4681 ggtccgagag caaagacagg atgttcttcg ccaacgcttc gttgtctttc ggcaacgaca
     4741 gcaaaccgga cttgccgtgc tcgacgagct gcggagtgcc gccactctcg agcgcaatca
     4801 cgggcttggc catggccatg gcttcgagga acgccaccgc acaaggctcc tcgaacgtcg
     4861 gcatggtgta gatgtccgtc ccggcaagca ggtttgcgat atcgctacgg aagccggtga
     4921 acgtgacgtt gtcggacagc cccagctccg cgctcaagct cttgatttgc gcgaggtaac
     4981 tgccgccccc cggcgtggcg cgcggatcat cctcaccgac gacgagcagc cgaaagttcg
     5041 ggttctgcgc tttgacgatc tgcagcgccc gcaacaggtc cgagtgcccc ttccaaggaa
     5101 acaggcgcga gatgatcgag agcaccggcg taccaggcgc aatcccgaat tcgcgccgta
     5161 cctcgctccc atcgagatcc gggttccaac ggcgagcatc gatgctgttc agcacgaaat
     5221 gcgtcttggc ggcgtcgtag cccgccgcga ccgcggactt cgccacgaag tcggagaccc
     5281 cgatcaaccc gtcattgtgc ttcatggccc agcgcaccag cggactgatc cagtcttcga
     5341 ccttgacgtg cacgtgggtg actgatcgcg ctccgatggc ccgcgcgagc agatgaccat
     5401 agaacgagtc gcgaggcttc tccgtgccat gcacgatttg gatgcggttc ttcttcgcat
     5461 aagaaaccaa gcccgccaaa ctcgccaaac ccgatgggcc cgactcccag agcaagcgcg
     5521 cggcttccga gaccgattcg ccatgaatgc tggggccgaa ctcggtgcgg cgaatcttga
     5581 tgccctcgat cttctcgaga gccttcaaag acgccgattt ctcaccggcc ttgccttgat
     5641 tgcaagccac atgcacctcg actcggccgc gatccaagta ctgcatcagc agactgtgaa
     5701 tcatcgaatc ggacccgaag aacgcttgcg actgcacgaa gagaacgcga atctcggaca
     5761 tcggaacctg tttcaatagc gttgcgctcg ccgcgcggtg ctagccaccg tcgtcgactc
     5821 gagctgcaag tgaactactt tccttacacg caaactttct gcgtgtaaaa gggaatttcc
     5881 gcgctcgcgc tggcattcac gccttgtctg tatccgcgcc gttatacccg tattcccttt
     5941 gcggccgcgg ttcgcagatg cgttcaacca aactgcctgg cacgtgttga ttgaattggt
     6001 tagattcgtt gattcggttg cctggtgctc cgctcgacag gcaacccaaa acgagaatgc
     6061 gagcccgcat gggtgaaaac ccagtttccg gattcacagc aatcgggggt agagaaaagt
     6121 cacagcgagc acataaagca ggttgaaaat agccatctgc ttgagcgctt ttctcaaacc
     6181 gcgctccgga tctttagtca ttgccgcgcg tgcggggatc acgatcgaca taatcaagat
     6241 agagagcaga acgagcttgg ccgaggcgct catgattctg tgtgggttct ttcgagggct
     6301 gtttcgaacg gactgttctt cgagtgggcc gtctgaatga gtacactctg aatggcgccg
     6361 cactgaagac cagcaacgtt ggttatgcgg cgtagccaga cggtccgcca ggccccgcgg
     6421 gcggcggcgc ggcttggggc ggggtggccg tctccagttt acccttggcg tggcgtccct
     6481 tcatgggcca tgccccggat attaccgcta gctttccggc gacggcaatc gacggcgcca
     6541 cgatgtagac ccccgtccaa gcccccaacc cgagatctcc gtaacactgg atcatgtaaa
     6601 tcaggaccgc gccgaaggac accaaagccg ccgcgcgatc catcgggttc gagagcgcac
     6661 ggtaagcccg tacgccaaag tacactcccc ccgtccagag cagcgtgatc atcgtgtatc
     6721 ccacgaagcc ggacgcaaac cagagcccga gcaggctatt gtgcggtaac cacggctcga
     6781 gcgggtgcgg catgggcggt aacccaatga tctgcagata ctgatgcccg taaccgagcc
     6841 cgaacagagg gttcgaacgg atcgtcatcg cgagatcgta gttttcgatg tcgcgcgtca
     6901 tcgtagacac gtcgttggcg ggctccacca ccgaacggat cgtacccacc ggcttgaaga
     6961 tgccggtcgg cgagccccac cccgccgcga tatacagcgc aatgaacggg ctcgcgtaga
     7021 gcagcgcatt gacgatcttc ttcttggcgc gcgttggcgg catcgagaag aacagggtga
     7081 ggaacaccag cgcgatttgc acccaaacca tgcgtcggtt gttctcgatc atgccccagc
     7141 agatcggcgg aaaaatggtg agcgcgaggc gccccgcgcg cggatgggcc ttgtgaatca
     7201 acaccgcgac caagatcacg acggcgcacg cgaacaacat cgagtcatga tggctcgtgg
     7261 cccagggtgg cttctccggg cccacgtcgg caaaatgcat cacgtatacg gccaccgccg
     7321 ctttgaccag agccgccgta atcacggtcc gaccgagcaa cagatagtct ttggggccac
     7381 gcaccgcgta ctggaacaat aggaacgtga tgggcagata agtgaccttc tcgatttgcc
     7441 aaagggactt ggagaagtct ccgcctgcga tcaagccgcg cccccacacg aacatcgcgc
     7501 ctgcgaggga cagtttggcc aaatcgatca aaggtttcgg cgtgggcgcc cacccgccgt
     7561 cgatcttgga tttactcgcc cggcgctgca gaaccacccc gatcaagaag atcaggaaca
     7621 tgtcggtccc cgagatcgac atgaagctca cgcccgtgac ctgattcatg tgggcgagca
     7681 acagcgcgcc cacgacgtaa agcggcgagc gccactgccc cgaggccgtc gcctcggacg
     7741 ggttctccag gctgaacgcg caaaacatca cccctacgag tgaataaatc accggcacgc
     7801 gtgtggcgaa ccagaacaac agcaacgtga ttgggacccc gagcagccag ccgacagcgg
     7861 cgttgtcgat ggccaccgcg accaacgtca aaaccaccgc tattgctacc gcagccttct
     7921 cgctcgcacc caacctagag ctcgccatac gcctcgttca ctcccgcgac tcggctgctc
     7981 gagccccacc tggctgcact cgggccctcg gccccggacg cggcagcgac ggcattcgtc
     8041 gcctcgacaa gtgcattgag tatcgaattt ctcacgctgg ctcgcgagct tagcccgatc
     8101 gcgtggtctc gcctaggggc agcaacggct tgaaatagcc tgaaagcgcc gatgaaccgc
     8161 tacgcgagca ccgaaagccg cgacgcggag gcagctccgc cggggaatcg cgcgcacagc
     8221 gtaacgagcg cgctgcgagt agcggggatg ctttcggtgt cttcgtccgt caaatccccc
     8281 agtgctaccg cgcctgcgcc acgccgcgaa cgcaccccca gcgacagtcc gtaacgctcg
     8341 aaactccggc gagccgtcac tcgacgggat ggcgacgcca ctcgtcactc tatcgagtga
     8401 ttgacggacc acgccccgac gagccgcgcg ttcctcccga cgtccttccc ggcgcagccc
     8461 cacagcgcgg aatcagattc ggcgcccgcg gtgttcgcgg tcacggcagc gccgaaagca
     8521 tcggcgctac cggtagagca cggaccgtaa ccggattacg gggcgagcgt gggcggcacc
     8581 atggccgcag gatcgggacc gctcccgtcg agcgcggtta ggaatgccac gagttgctcg
     8641 cgttcctcgc gcgaaagatt gcgcggagcg ttctcggcaa cgccaacgaa gccgccgctg
     8701 gacgcgcctt cgttgaagaa gtccaccacg tccccgagcg agcgaaactc ggcgttgtgc
     8761 atgaagctgc cccgtcggct aacgcaacgc aggcccggcg tcttgaagga gccgagcagt
     8821 gcctggggat cagcgggcag cttgtccaag cgacggtcgt ctccgtcgct gaagacaccg
     8881 cggctattca atggatcctt cagcaacacg gctacagccg gagctgcacc cggatcattg
     8941 aaggtcgcta cacccgtgaa aggcacgagc ccaccagaca ggcccacgtt gtggaagcgc
     9001 ccgtccgtga ggtacgggcc ggagtgacac atggagcagc cggctttgcc cacgaagagc
     9061 agcgcgcccg ctttctcctg atcggagaga gcggtctcgg tgccttcgag ccactcgtcg
     9121 aaacgggagc gtccacagct gagtgttctg gtgaaggcct ggacggcttt gccgaggttc
     9181 gcgaccactc gcgtgaccgg ttcgtcctga tcgccgggct ttccgcactg gccggagacc
     9241 gggtcagacg gcaattcact gcacccggcc tggctcgcat cgagggccgg atacttcgac
     9301 agatccggca tcgccccgaa aaccgcttcg tattcagcgc ggtagaggcg agccacctgc
     9361 tgggcaacga atagacgcga gctattgaac tcgagggggc tctcgatcgg cgaaaagact
     9421 tggctgaaga cgctgtcgcg tcggccatcc cacatgaaga actgcccctg cgccacgtcc
     9481 aaaatcgacg gagctttgcg gtgagtccag cccgagccca aggacagctg ctgccgcgga
     9541 cttcgcagat cggcgaacgc gccgccgtcg gtgacgtggc agctcacgca gcctactttg
     9601 ccggtctcac cttgcatccc gagcgtgcca ctcgtgccgt tattggcttg atccagcagc
     9661 ggaccggaga agcgcgtttc atagaagaac ttctttccga gtgccgccgc gcgcggatca
     9721 tccgcgtagc gattggtcac atcgacgggt gctttggcgt ccttgggcaa cagcaacgtt
     9781 ttcaaccgcg ccatcggatc gtccatgacc ggtggcggca tggtgctcgg agcgccgctc
     9841 ggactcgcgg tggccgtcga gctcgggctc gccgaaggga gggcgccggg atcgcaggtc
     9901 acgcacgcac cgcgcacgtt ttggctgcat gcgccgagca ccagcgcgac tccggcgaac
     9961 aacgccgggg tgaacccaag tccgagccca cggcgagggc cgtcgaacga cgaaccctca
    10021 ccgctcaccg attcgttact gcgcatcgat tgtgagatcc atgttgccga cgtactcaat
    10081 cggcttgtct tgctgcggct gatccatctc gagcttgttc acgcccgcca ccagctcgga
    10141 gagcggaacc tcgaccgaga acgaacgcag tgtaatctgc gaatcgaact cggagggagt
    10201 catgaacgtg tgccactctt tgccgttgaa tcgataacga aacgccttgt tccccgcggc
    10261 gtgcaggttg aatccgaaga tggcgcgctt ggccgcgctc agatccacgt ctttgacgct
    10321 caccgagacc ggattggaca gcgagtagcc cacctgatgc tcttggggct tcgcgctgta
    10381 accatcgtcg tcgatctcgt aggcgcgcgg cttggcctgc ttcgggccat cgaaaccgat
    10441 gttgtcccaa cggaaggtct gggaagaaga agcgttggcc ttgctggcgt tgtattgcgc
    10501 gtgctgaatg tgcacgtagc cgaccgagaa ccgcagaccg agctccttca cgactgcgac
    10561 gcggcgtagg gtgttcgggt tgtcgaagtc actcgcccag aactccgccg agtcttgcga
    10621 gatcaggagc ttgaagcggt tacccttacc gtccgcgacc ttgaagccgc ccgagtcgct
    10681 gatcacggtg tcgcgcgtga gcttgtagtc tttcgacacg atcacgcgac cgacctggtt
    10741 acaaccgacg tcgccgaagc atgagccgcc cgcgggcgcg atctggaagc ccaccgcgtc
    10801 cgtcacgtag gaagcaaccg tgggtgcacc gtgatatggc atcggcacgg gttccttgga
    10861 gatccaaatc tcggtccacc aaccgtgccc gtcgtagtcc ttgttgcgct tggcatcgac
    10921 ctcgaacgcc accgtgccca cgcgtcccgt gaagtcgaac ggctggcgaa tacgcatcga
    10981 cgtgaagccg aacgcgccgg catcgttcat gacctcctcg agccgacgcg agccaccttc
    11041 ggaggcgcac agccggatat cattcggcgg caacaaattg gagaactgct gaccgcacaa
    11101 cgtcggggtg ttctccacgc cggactcggt gctggtcgca gccaggcgtg tgaagatcgc
    11161 gatcgtcgag gcaatcccgt agcgcgatac gctccacaca ttctcatcca acggcccgcc
    11221 acggccaccg ggtttgtctg ccccttcgaa gtcttcgcag aacgcgggat tctcgagccc
    11281 gcacggaccg gaggcttgga ccggccccgc tccggcgacg ttggtgctgc cgccaggaga
    11341 ggtggcccca ccggccgcat tggagccgcc attggcagca ccggaggtgt tgttggcacc
    11401 cgctgagctc gaaccagcga cgttggcgcc tgcgctacca cctgcggccg cgcctccgct
    11461 caggctgccg ccggaagaaa cgttgccacc agccgatagg ccgccttcgc ccgcggggag
    11521 acctgcggtc ccggcagtcg gcgcttcggg cccttgttgt tgacctgaac agccagctga
    11581 caatgcacaa acaatcaggg ttgcccaatt tgcaacgctc gtgacgcgta ttcccacggt
    11641 gctctcctta gcaattcctc gcccaatggc acgcccgaga ttgctctcga gcttctcccg
    11701 cgaggttgac cgcgacctca cgtgacaatc caaaaatcag aagctttctt tcaggaacaa
    11761 cctccgctcg gaaggtgtgc ccctcgataa gccttgcgct gccggccact ctcagtggcc
    11821 gtcctctaca gcccggcttc ggcaagctca gtcggttgtg aacgaccctt tgggtcccga
    11881 cttaattgca gcctgttggc tgcacccgct cgcgctcttc gctctgccaa ccttcccaga
    11941 aggctaactc ccggagcagg ctgcctttcg cgggatttct cgctgggctc gctgcatcga
    12001 tgagcccgcg cgctgtctcc tgttcactgc agctacggag ccgcttcaag ctttctctca
    12061 tgagcatttc ttttgcgtga ccagcctctg cgcttgtgtc ttccgctccg ctgtttctct
    12121 gccgctgcgc cgtttctctg ccgctccgcc gtttctctgc cgcttctgcg gctctgtctt
    12181 ccgtggcgct gtctgcggcg gggctgtctt tcgctcttct ggggcgctct tcgttagggc
    12241 cgattctgcg ctgtcacgca ctgcgaccgc tgtaaggcga atgccgtcga aaagcccctt
    12301 atcgtacttt gccgtcggct gagtccagcc gttcggcaaa aatccagctc tcatccaacc
    12361 aagcccgact cgacattacc cagaatcact gcgagtgccg cgaacccgag agctggtcgt
    12421 ttttcacccg cggagcttga ggcatgtcga gctgcaccgc aagttgcgac acaccgattg
    12481 gcagcaggaa gtgattgaac cgaccacacc gcgaaactcg tgcagaacat gaaacaagct
    12541 gctcgcaaaa cggttcgcgc gatgaaacaa ccacgcgcgg ttcaggctcg ttgcgcaaag
    12601 cctcgaatcg tcgtgcgcgc ccgcccagtc tgcttactgt cgacctcagc caatttgaat
    12661 actctgagat cttttgcata tccccgcata tttcagggcc tgtggtgccg tgatgtgcgc
    12721 tcatgaccaa ccggtgacac gcgctggcac tctgaacagg cactctgcgt tgcgcatcgg
    12781 ctctggtcac cgcaggtcgc aattcgctca tcacatgcgg cgcgcgcaac tggtttgctg
    12841 agttccgact ctcggcgccg aacgcgagcc acccgcgccc cgcaattcct ccgcggagag
    12901 tcctcacgcc cgctcgcacg ccgcgcgcga aaccatcacg agcgcgtggg ctggcaagct
    12961 ccgtgaacgg ccggcgtgtg agcgtgtccg cttcgatcgc gtgacccacg gcgtgacagt
    13021 caccaacgat ccggcatgtt ttaaggctat ttttacgcgc acgtgacgct tcgccgcgcg
    13081 gcgatttgcg cgagcttcgt ccgcgcggcc ttgcctttcc gcggagcaag tgagaaacag
    13141 gtccggtggc agaaatgtca agcacgggcg caagaagaag aatccttttc gtgtctccga
    13201 cgacagccgc aggaggtggc gcagagaggg tgttgctcga ccaccagcgc ttcctggatc
    13261 gctcgcgttt cgagccggtg attgccgtgc ttgggcaggg caccttcgcg gaagaggcca
    13321 agtcgctcgg gatcgaggtg tatgtggcgg gcccgcattc ggatcgtcaa ccgctggcca
    13381 cggcgcgcga gctctacaac ttcggccggt tcgtgcgtga tcgtcggatc gatgtgctcg
    13441 tcggcaataa gtaccgctcg atcttctact ggggcctgag ccaggcgcgc cgacggcctt
    13501 tcgtttggtt gctgcacgat ccgctgcccc aggccgggct gcaccgcaaa gtgctcgcga
    13561 ccgtcaccga ccagctgcga ccgagtcata ccgtctgggt gacaccggaa tcgagcgaaa
    13621 gctacgggca gaggttcccg cacctgctcc agagttcgtc gcaaattcgc cccggcacct
    13681 gccccgaaga cttgatcgag ggcgctgacg cgcagcgcgc ccgtcgtcgt tggggcattc
    13741 cggtggatgc accgattgcg gcgatgtttg cgcggatgca ggagtccaag ggtcacttgg
    13801 atttggtgca cgcggccgcg acggtcttga aagaataccc ggagacgcgg ttcctgctgt
    13861 gtggcgggac gctcccgaac atggatccga gccatgaaac caacgtaaag cgcgcaatcg
    13921 ccgagctcgg ggtcagtgat cgggttctaa cccttgggat tatcagcgaa gaggaaaaga
    13981 aggatattct ggcggccagc accttgctcg tacacccggc cgtctgggag ccatttggga
    14041 ttgccgtgat cgagggcatg gcggtgggca aaccggtggt ggtggccgat gctcgcgggc
    14101 cgggcttgat cgtggagcac ggccagaccg gcttggtggt gccgcgttcc cagccggcgg
    14161 ccctggccga aggaatttcg aggctcttgc gagacccaga gttggctcgg gagatgggtg
    14221 aaaggggcgc cgaacgggtt cgagagcgct atcacgcgcg ggtcgccacc aagcggctgg
    14281 aggacatctt cgatggctta cctcctcttg ccgcatcagc cggggcaggc tgagcccgag
    14341 ccatgagcgc cagcaacgcc gaggcggccg agccgcagca acccgcaccg cctaccgtgc
    14401 cgcccaaccc cgaaccgcaa gagccagggc acgagcagcg taaagccacc ggcagcctcg
    14461 tgctgcgcaa caccctgttc ttgatgggtg ctcagatcgt caatacgccg ttgtcgattc
    14521 tgatcaacgt gatgatgggg cgctatctcg gccccaatga gttcggtctg ctgtacctgg
    14581 cgtctacgtt ttgttccttc ggctttttgt tcgtagattg ggggcaaagc agcgtcgtgc
    14641 ccgcgattgt ggcccgcgaa cgcagcagcg tgaatggcgt actcggtagc agcctgttgt
    14701 ttcgactggc ggccgcttgc atcgtttatg tgttcatggc gccgctggcg ctgatcatcg
    14761 cgcccgggca tggctttcag ctgacgctcg tgatcgtcgc cgcgctcgcg ctcgtgggca
    14821 gcctgtattc cgccttcttg gacacgctcc gtggcctcga gcggctcgat ctctccgcgt
    14881 acaacggggt tggtcagcag ctcttgacgg gcgcgttggt catcccgatc ttgctgttgg
    14941 gtggccggct gcatagcgtg cttttggcgc aaatcgccgc cgtggtggtg gtgctggcgg
    15001 tagccgtgaa gatcttgggc ggcctcggcg tgcgcccagc gcggcctcac atgagccaca
    15061 tcaagcgcct gctgcacgag ggaactccgt tcgtgttctt cggctttgcg atggctctgc
    15121 agcccaacgt ggacgcggga ttcctctcgc gcatggtccc caccgaagtg attggttggc
    15181 acgccgccgc ccgacgcctg ctgggcttct tggtcactcc ggcaggggct ttgattggcg
    15241 cgctctatcc aaccctctgt cgattgcatg ccgaagaccg cgagagcttc gtcaacgtca
    15301 cgcgacaagg cgtccagagc gcgctgctgt tggccacgcc gattgctttg ggctgcgcgc
    15361 tctacgcgga tatcggagtg gccatttaca gtaaagaagc ctttggccct gccgcgatga
    15421 atctgcggct gctcgcgccc ttcattctat tggtttacgt gagcatgccg ctcggtagct
    15481 cactgctggc cgccggtcgg cagcgcactt gggcgggggt ccagctggtc tgcgtgggtg
    15541 tcagtctgat tttggacccg ttgctcgtgc ccttgatgca gcggcgctac gcgaacggcg
    15601 gcctcggcgt ctgtatcgcg acggtcgcga gcgagctggt catggtcgtt tgcggggtgt
    15661 ggatgacgcc ccgaggcatc tttgatcgtt cctggctgcg caagctactc ccgctgctcg
    15721 cgggggcagc gggcatgacc gcgaccgccc tggctttggc cgcggtgtct ttgacgccct
    15781 acgtggcagc ccctctcgcg gtggcagctt acggcggaac gatttgggct acgggcggag
    15841 tcgatccggt ctacgtcgag aagttcaagg ccaagctgcg cagcaaactc aagcgttagg
    15901 ccgagagcac caggagcgcc atcgactcgg ggggcagcac gagctcctgg ccctcgatcg
    15961 agggcgcccc catcccgtag atgacctcga agccccgatc cgattcacca actttgttgg
    16021 gagcaggttt gttcgggaga ggccccgctg gaatacgcac cgcctcggag ccgaagttca
    16081 cggccacgcg gctgcgctcg cggtcgatca gcagggtccc gcgcctctcg tcggcgtagg
    16141 cgtgcacaca ggcggaagag agctccggcc tgctacgccg cagggccacg agcgcacggt
    16201 actgctccag aatggccgcg tgctccggca gctcgagctc gcgaaaattg agccgactcg
    16261 cctcgaacgt ctctggagcc tgagggtcgg gaacggcttc cgcgggccag ccgaactccg
    16321 caaactctcg ccttcgtccc tcgctgaccg cacgcgcgag ctctgggttg tgatgctcgg
    16381 tgaagtattg aaatggcgag cgggcccccc actcttcgcc ctgaaacagc atcggcacac
    16441 tcggcccgag caaagtcacg gccgcgccca acagcgcgcg gcgcggggaa atgaggtgcg
    16501 taatccgctc accgcgcgcg cggttaccaa cttggtcgtg attctgcatt gcgaccacga
    16561 gccttcggag cgacaagttt tcgaacgggg ccccgtgaca acgctgccga aatgcgctgt
    16621 aacgcccgtc gacgacgaag ccgcgctcca gcgccttggc caaatcgcga atgccgaaaa
    16681 aatccccgta gatcccgttg cgctcaccag tcaaagtgac gtgcagtgcg tggtggaagt
    16741 cgtcactcca ctgcgcgtcg aggccgtagc ctcctgcgtc cgaggaatgc accattctgg
    16801 gatcgttcaa gtcgctctcg gcaatcaaga tcagcggacg ttgctcgcga ttggaaagct
    16861 cgagcgtttc ctccgccaac tgtttgagga agtgacgggc ggaacggtcg aagatcgaat
    16921 gcacggcgtc gagcctcaag ccatcgaagc ggaactcgga catccactgc agggcgcagt
    16981 cgatgaagaa acgccgcacg tgatccgagc gcggcccgtc catgttgacc gccgcgcccc
    17041 atggcgtgtg gaacgtatcg accagatagg ggccaaatcg cggcaaatag ttcccgtccg
    17101 gcccaaagtg gttgaaaacc acgtccagca ggatgcccat gccttgcgcg tgacaggcgt
    17161 tgaccagccg cttcaaagcc agagggcccc catagccgct atgagcggcg aacggataaa
    17221 cgccgtcgta accccaccca cgcgtgcccg ggaactccgc gattggcatg agctccacgt
    17281 gggtcacccc gagcgaacgc aggtgcggaa ggcgtgggat cacgccatcg aacgtcccgt
    17341 cccctgcaaa cgtgcccacg tgtaactcgt acaccacacc cgaagccagc ggcggagctc
    17401 taaagcccga gtcggtgaac tcgaactggg tgtcgagaat gcgcgagggg ccgtgcacgc
    17461 cacgcggttg ccagcgcgag cgcggatcgg gcaacggtgg accgccgttc aaggagaact
    17521 ggtaatcggt gcccggaggc aggtccgggc cacagaatcg atcgccgtcg attcgcttca
    17581 tttcgatgcg agactcaccc gtaacgagtt caaccttttc ggtgttcggc gcccagaccc
    17641 acactgttga cacgtgcatc tcctccggtg actgcaggaa atccaagcaa ttcgtgtgcc
    17701 cggatcctag tcctcgagtg cgtagaacat gcccgcgcat acgcggaaca cgcctaaggc
    17761 gccatgtacg tgaatcaacg taccggccac ggcatacacg cggttccgcc cgtttcgtag
    17821 gactccccgt ccgcgcgccc tgttgtggcg ttcgcgcgga gtcgagtgtt gcttttggcc
    17881 ctaaaactcg ggaatttgag ccgttgccgt gggcagtcct tgccgagtct ctgatggccg
    17941 tttcctcaat cgatcgcgtt actgggatag gttcttgatg aagtgccgtc caagctgacc
    18001 agcacgagcc ggcacagggc atcgttcagt actcgcgcga ggcgttgcta gccatcgact
    18061 tgcaggccat cacccacccc gacgctctcg acgcgacttg accctcgtgc agcaatagct
    18121 gcgcggcgag ctggaatgta tcgtaaaccc cgcctgggag agcaccctgg gcttcagcgc
    18181 ggaagaccgc gtgctcgcac gcgcggctcg agcgctctcc gccgcgaaat ccgcggggcg
    18241 agactgcttc cgcgtggctt cggagtgaag tccggctgag ggcgcaggcg cggtttcgag
    18301 ggtgaagtag gggcagtaag actgcgcgag ggtcgaggtt ggacgcgctt cgcgcacgtg
    18361 attttgcggc gacagaccag agacttctcg aggaaacacg ccgcgccaac gctggctact
    18421 cctgagcgga cgggatt
//
LOCUS       DASTKU010000931         3573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_181198, whole genome shotgun sequence.
ACCESSION   DASTKU010000931 DASTKU010000000
VERSION     DASTKU010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181198"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VFQ61_32945"
     CDS             complement(<1..840)
                     /locus_tag="VFQ61_32945"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9959360.1"
                     /translation="MTKHYEFLLNGEAIDLRQAIEAVGGDPHITLLQWLRATGRTGTK
                     EGCAEGECGACAVVVRRIEAGQASGTFEAVNSCILPLLALEGAEVTSVEGVSPRSSTP
                     NDADGALHPVQRALVERGGSQCGYCTPGFVMGLFTEYYRPGREGFDPACVSGNLCRCT
                     GYRPILEAGRSLPLPEASDPRLAELRRPGRVALSVVYENSGRHFVRPTSWQELWDALD
                     RFPEHTLIHGGTDLMVYANQRYRRYPVLISLEGLAELRELHFTEEFVSLGAGASLSRI
                     DRWL"
     gene            895..1770
                     /locus_tag="VFQ61_32950"
     CDS             895..1770
                     /locus_tag="VFQ61_32950"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET9959361.1"
                     /translation="MRAILQALSELLEQRRRGALATVVRTSGSVPQVAGARLLLRDDG
                     TSFGTVGGGAIEHSVREALERVLATGRPELLQRDLAHDLGMCCGGRMEVFVERVEPPP
                     RLWLIGAGHVALATAPLARRVGFEVSVVDEREELNSAVRFPDCELRLAEPSEVLRKAS
                     FTPFDWILIATHDHQLDEQSLHAALATSARYIGLVGSRRKVLRLVQRVALKSATPPSL
                     ERLFAPVGLDIGAVSPEEIAVSIVAELVAVRNSHPAPHLRTPELARAVSAQAIGDELG
                     AVLGLPNNPTKPPPA"
     gene            1767..3173
                     /locus_tag="VFQ61_32955"
     CDS             1767..3173
                     /locus_tag="VFQ61_32955"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9959362.1"
                     /translation="MSLETLSRQVEYELELLDYPRRPWLEARQTAGGGGIYDVVIVGA
                     GQSGLTTAFGLARERVDNVLVLDENPLDHAGPWLSFARMQTLRTPKYVTGPDLGVPSL
                     TPRAYYEAQFGMGSWERLGLIPKQVWADYLAWYRQVLKLPVRANTRVGPIRFDSTAGA
                     FELPCRDTTSAAPPNSIYARRVVLATGIDGCGEWRVPRVVHDSLPPELYAHTRAHIDF
                     ASLAGKRVVVLGAGASAFDNAATALEHGAREVHLLFRRDTLVNVNAYRWAEFAGFLKH
                     CADLPDPQKWRFILQILRMGQLPPADTLARAKQHAQFHMHPGCMWRSLEARGGCALIH
                     TEARTFEADFVIVATGFVTDLKARPELRHLEPFIARWSDRYTPPEADRHADLSNHPYL
                     GSNFELTERVPGSAPYLSQVYNYTFGSLLSLGFGGASISGLKYSAPRLIAGITRSLFL
                     EDSALHFERLCQFAEREF"
     gene            3173..>3573
                     /locus_tag="VFQ61_32960"
     CDS             3173..>3573
                     /locus_tag="VFQ61_32960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET9959363.1"
                     /translation="MGFTALRSSRVVCPEGVQPATVLIENERIVQVLPGRVDVPGVQD
                     FGDLVIGPGLVDCHVHVNEPGRSEWEGFATATRAAAAGGVTALVDMPLNSSPVTTTPL
                     AFLQKLEASRGQCFVDVGFWGGVVPGNGSVLG"
BASE COUNT          612 a         1184 c         1135 g          642 t
ORIGIN      
        1 aagccagcga tcgatgcgcg ataacgaggc gcccgcaccg aggctcacga actcttccgt
       61 gaagtgcagc tcgcgcagtt ccgccaaccc ctctagtgaa atcaagacgg ggtaacgccg
      121 gtagcgctga ttggcataga ccatcaaatc agtgccgccg tggatcagtg tgtgttcggg
      181 gaaacggtcc agcgcatccc acagctcttg ccacgaggtg gggcgcacga agtgccgccc
      241 ggaattctcg tacaccaccg aaagcgctac gcgcccggga cgccggagtt ccgccagccg
      301 tgggtcgctc gcttctggga ggggtaagct gcggccagcc tccaggatgg gccgatagcc
      361 cgtgcatcgg cacaggttgc cagagacgca cgccgggtcg aagccctcgc ggccggggcg
      421 gtagtattcg gtgaacaagc ccatcacgaa gccgggcgtg caatatccgc actgcgaacc
      481 cccgcgctcg accaaagcgc gttgcaccgg atgcagggcg ccgtctgcat cgttgggcgt
      541 cgagctacgg ggtgaaacac cttcgacgct ggtgacttct gctccttcga gcgcgagcag
      601 cggcaatatg cagctgttga ccgcttcgaa agtgcccgag gcctggccgg cttcgatgcg
      661 gcgcaccacg acggcacagg cgccgcattc gccttccgcg cagccttctt tcgtgcccgt
      721 gcgaccggtc gcgcgcagcc attgcaacag cgtgatgtgc ggatccccgc cgacggcctc
      781 gattgcctgg cgcaaatcga tggcctctcc gttcaacaag aactcgtaat gtttcgtcac
      841 agtcggtgct cggaaacacc cacgacacgc acgaactgtc acataatacg cagcgtgcgc
      901 gcgatccttc aagcgctatc agagcttctc gaacaacgtc ggcgcggcgc cctcgccact
      961 gtcgttcgta cctcgggctc ggtgccccag gtcgccgggg cccgcctctt gctgcgtgac
     1021 gatggcacga gcttcggtac ggtgggcggg ggcgccatcg aacacagcgt tcgggaggcc
     1081 ttggagcgcg tgctggccac gggtcgaccc gagctgctgc agcgcgacct cgctcacgat
     1141 ctagggatgt gctgtggtgg gcgaatggag gtgttcgtgg agcgagtcga gccacctccc
     1201 cggctctggc taatcggggc gggccacgtc gccctagcca cggcgcctct ggcgcgccgc
     1261 gtggggttcg aagttagcgt cgtggatgag cgagaagagc tcaactcagc cgtgcgtttc
     1321 cccgattgcg agctgcggct cgcggagccg agcgaagtcc tgcgcaaagc aagtttcacc
     1381 ccattcgatt ggatcctgat tgccacccac gatcatcagc tcgacgaaca gagcttgcac
     1441 gccgccctcg cgaccagcgc gcgttatatc ggcctggtcg ggagccgcag aaaagtcctg
     1501 cgcctggtgc aacgggtagc cctcaagagc gcgacgcccc cgagcctcga gcgtctattt
     1561 gcgcccgtcg ggctcgacat tggtgcggtc agtcccgaag aaatcgcagt gagcatcgtg
     1621 gccgagctcg tcgcagtgcg aaattctcat cccgctccgc acctgcggac gcccgaactc
     1681 gcgcgcgcag tcagcgcaca ggcgattggg gacgaactcg gcgccgtcct cggccttcct
     1741 aacaacccta ccaaaccacc tcccgcatga gcctcgaaac cctgagccgc caagtggagt
     1801 acgaactcga actgctggac tacccgaggc ggccttggct cgaagcgcgg caaaccgctg
     1861 gcggaggggg catctacgac gtggtgatcg ttggcgcggg ccagagcggg ttgaccacgg
     1921 cgttcgggct tgcccgcgaa cgcgtcgaca acgtcctggt gctggacgag aacccgctcg
     1981 atcacgcagg cccttggctc agcttcgcgc gcatgcagac cctgcgcaca ccgaagtacg
     2041 tcaccggccc cgacttgggc gttccaagcc tcacgccccg cgcctactac gaggcgcagt
     2101 tcggtatggg cagctgggaa aggctggggt tgatcccgaa gcaggtctgg gccgactact
     2161 tggcttggta tcgccaagtc ttgaagttgc cagtccgcgc caacacgcgc gtgggcccca
     2221 tccgtttcga ctcgaccgcg ggggctttcg agctgccctg tcgtgacacc acgagcgctg
     2281 cacccccgaa ctcgatctac gcgcggcgcg tggtgcttgc caccggcatc gacggctgcg
     2341 gtgaatggcg cgtgccgcgc gtcgttcacg acagtttacc cccagagctt tacgcccata
     2401 cgcgcgcgca catcgatttc gcctcgctcg ccggcaagcg cgtcgtcgtc ctgggcgcgg
     2461 gcgcctctgc cttcgacaac gccgccacgg cgctcgagca cggcgcccgc gaagtgcacc
     2521 tgctattccg gcgtgacacg ttggtgaacg tcaacgccta tcgctgggcg gagttcgcag
     2581 gcttcttgaa gcactgtgct gacttgccag atccccaaaa gtggcgtttc attcttcaga
     2641 tcctgcgcat gggccagctg ccacccgccg atactttggc gcgtgccaaa cagcacgctc
     2701 aattccacat gcatccaggt tgcatgtgga gaagcctcga ggcccgcggc ggttgcgcgt
     2761 tgatccacac cgaagctcgc accttcgaag ccgatttcgt gatcgtggcg accgggttcg
     2821 ttaccgacct caaggctcga cccgagctcc gacacctcga accgttcatc gcgcgatggt
     2881 cagaccgcta cacccccccc gaggccgatc ggcacgcgga cctttcgaac cacccgtact
     2941 tgggctccaa tttcgagctg acggagcggg tgcccggcag cgcgccctac ctatctcagg
     3001 tctacaacta tactttcggc agtttactca gcttgggctt tggcggcgcg agcatctccg
     3061 gcttgaagta ctccgcgccg cgcttgattg caggcattac tcgctccttg ttcttggaag
     3121 atagcgcgct gcatttcgag agattgtgcc agtttgccga gagggagttc tgatgggatt
     3181 tactgcgttg cgcagctcgc gcgtcgtctg ccccgagggc gttcagccgg ccacggtgtt
     3241 gatcgagaac gagcgcatcg tgcaagtact gccgggccgg gtggacgtgc ccggcgtgca
     3301 ggactttggg gatctggtga ttggtccggg cctcgtggat tgccacgttc acgtgaacga
     3361 acccgggcgc agcgaatggg aggggttcgc caccgccacg cgcgcggcgg ccgcgggcgg
     3421 agtcaccgcg ctggtggaca tgcccctcaa ctcgagtccg gtgaccacca cgccgctcgc
     3481 attcctgcaa aagctcgagg ccagtcgtgg ccagtgcttc gtcgacgtgg gcttctgggg
     3541 cggcgtggtg ccgggaaacg gctcggtgct cgg
//
LOCUS       DASTKU010000932         4410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1969390, whole genome shotgun sequence.
ACCESSION   DASTKU010000932 DASTKU010000000
VERSION     DASTKU010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969390"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VFQ61_32965"
     CDS             <1..1083
                     /locus_tag="VFQ61_32965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959364.1"
                     /translation="QALLGIYPDLFMGGVAISGVPCGCWAASYTGDSGSSAQWSGPCA
                     GGQVSKSAQEWGDQVRAMFPGYTGHRPRVQLWHGKADTTINYNNQVEAIKEWTNLLAL
                     SATPTVTDTAKAGQTHQTWKSACGYTVLETFALDGIGHSVNWDLNTAAKFLGLDQPVT
                     TDPEAAACPSNGTGGAGGVSGAGGAAGSGGSSGGRSASGGAMSAGGSAGNSSPGGSTS
                     TAGGAGAAASGGAPSASGGNSSGGSTNTAGNNSGGAVAQSGALGAGGASAAAGASNPA
                     MGTGGGASGGSNTSGGSNGGQNTTGTSPAQVGGSAVAGAPSAGVDASSGCGCRVVSPK
                     RSLGAMGALAFVAAAFGLRRARKRRE"
     gene            complement(1240..1545)
                     /locus_tag="VFQ61_32970"
     CDS             complement(1240..1545)
                     /locus_tag="VFQ61_32970"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF016120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET9959365.1"
                     /translation="MTPALERARDAGVVRLLAHGAETGYSLFESADGRFVSHLGHPEY
                     EAQRLSLEWARDSQLGRSDVDPPKNFDVNRPANVWRAHCNQLFDSWLERIAAGAPTS"
     gene            1565..1828
                     /locus_tag="VFQ61_32975"
     CDS             1565..1828
                     /locus_tag="VFQ61_32975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959366.1"
                     /translation="MRAPEPVVELVRQRMVRVQALVLEYAEKLTLEDALLDTEDGAEG
                     ESTPAQPQGARDLAAGVAENLAQFGPIVDRLELELFHGRAGQD"
     gene            complement(2122..3408)
                     /locus_tag="VFQ61_32980"
     CDS             complement(2122..3408)
                     /locus_tag="VFQ61_32980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HET9959367.1"
                     /translation="MPHPAFKPETLALHGGQDPDPTTGSRAVPIHQTTSYQFKDTEHA
                     ANLFALKEFGWIYTRLMNPTTDVFERRIAALDGGAGALAVASGQSAISLSLLNLARAG
                     DEIVSADNLYGGTYNLFHYTFPRLGIKVRFVPSNDLDALAKAITPRVKAVYAESIGNP
                     KLDVAELEGIAKIAHANGVPFVLDNTVSPYLLRPFNHGVDIAVYSATKFIGGHGTSIG
                     GVIVDSGKFEWTQDKHPLIAGPEPSYHGLNFVEALKPLGNIAYIVKARVTLLRDLGPA
                     LSPFNSFLFLQGLETLPLRLIKHSENALAVARYLATHKKVRWVNYPGLEESSERERVR
                     KYLPKGAGAILGFGVEGGAEAGRRFIDRLNLLSHVANVGDAKSLAIHPATTTHQQLSL
                     EEQRATGVTPDYVRLSLGLEHVDDLIADIEQALEKA"
     gene            complement(3634..4257)
                     /locus_tag="VFQ61_32985"
     CDS             complement(3634..4257)
                     /locus_tag="VFQ61_32985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959368.1"
                     /translation="MAHALRVSAHELLGGTRKLLQQLLPVDGSVHLVSVAAARSAGIH
                     GGMSSPRAAKAQCDDKQFDTPQSSEPRRNVRPAAACRDEATRVRLLTQLVAFRRAHEA
                     ARQRVAAAELEGIFPYRTYRMRQMVHDPRASAAKLAELSPGLGCEARGQGGTAARGAE
                     LRERGALRLRRRAMAFRLMRDLRIGAAWSHGTCGLLADFEGSLVGLQ"
BASE COUNT          829 a         1341 c         1461 g          779 t
ORIGIN      
        1 caagcgttgc tcgggatcta cccggacctg ttcatgggag gtgtcgccat atcgggcgtg
       61 ccctgcggat gttgggccgc aagctacacg ggagactccg ggtcgagcgc gcagtggagt
      121 ggcccgtgcg ctggcggcca ggtctcgaag agcgctcaag agtggggaga ccaagttcgc
      181 gccatgttcc ccggttacac gggtcatcgc ccgcgagttc agctctggca cggcaaggcg
      241 gacacgacca tcaattacaa taaccaagtc gaggccatca aagagtggac gaacttgctc
      301 gctctgagcg ccactcctac cgttacggat actgccaagg cggggcaaac ccatcagacc
      361 tggaagagcg catgcgggta tacggtactc gagacgttcg cactcgatgg cattgggcac
      421 agtgtgaatt gggacttgaa cacggccgcg aagtttctgg ggctggacca gccggtcact
      481 accgatccgg aagcagcagc gtgccccagc aacggtacgg gcggagcggg tggagtgagt
      541 ggagcgggtg gggccgctgg tagcggcgga tcgagtggcg ggcgttccgc gagcggcggc
      601 gccatgagcg ccggcggcag cgccggtaac tcgagtccag gtggctcgac gagcacagct
      661 ggcggagcgg gcgccgctgc cagcggcggt gctccgagcg cgagcggtgg caactcctcg
      721 ggaggaagca ccaacaccgc cgggaacaac agcggaggag cggtagcgca aagcggcgcg
      781 ctcggcgcgg gaggagcctc ggccgcagca ggcgccagca atcccgcaat ggggaccggc
      841 ggaggcgcgt ccggtggctc gaacacgagc ggaggaagca acggtggcca gaacacgacc
      901 ggaacgagtc cggcgcaggt gggtgggagt gccgtcgcgg gcgcaccgag cgctggcgtg
      961 gacgcttcct cgggttgcgg ctgccgagtt gtgtcgccca agcgatctct cggcgcaatg
     1021 ggcgcgctcg cattcgtcgc cgcggcattc ggcttgcgtc gcgccaggaa acgtcgcgag
     1081 tgatcccgta cgagggttac ctccaaagca gcgagcaggt cccccttggc cgcgtgcctc
     1141 gcggccccgt ccaggcgtcg acgctgctgg aatcagaagc actgggccgt ccgatcgtcg
     1201 ggtcgcagca ggaattgccg ccgcacgctg ctccaaagat caactcgtcg gcgctccggc
     1261 tgcgatgcgc tcaagccagc tatcgaagag ctggttgcag tgcgcacgcc agacattggc
     1321 cgggcggttg acgtcgaagt tcttgggcgg atctacgtct gaccgaccga gctgactatc
     1381 gcgggcccac tctagactca agcgctgtgc ctcgtactcc ggatgtccga gatgggatac
     1441 gaagcgcccg tccgcactct cgaacagcga gtatccagtc tcggccccgt gtgcgagtag
     1501 acgcaccacc cccgcatcgc gcgctcgttc gagtgcgggc gtcatccaca ccggagtgcc
     1561 ggctgtgcgc gcaccagaac cggtcgtcga actcgtgcgt caacggatgg tcagggtcca
     1621 ggcactcgtt ctggaatacg ccgaaaagct tacgctggaa gacgcgcttc tggataccga
     1681 ggacggagcc gagggcgagt ccaccccagc acaaccccaa ggtgctcggg atctcgcggc
     1741 gggcgtagcc gagaatctcg ctcagttcgg cccaatagtg gaccgactcg aactcgagct
     1801 cttccacggg cgcgccggtc aggattaaac cgtcgatggg cccctgactc agcgcgtgct
     1861 cgaaggtgat gtagtgcgcc gcgatgtgct cggcgttgct gcttccgtag ccatggctac
     1921 ggagtcgaat ccaacgtgta tccaccggaa tccgggcacg gcggaggggc cggatcaaat
     1981 aagactcata agtctccgca cgtggcatga tgttgactat tgccacgcga agcgcgcgtt
     2041 taggctcgat cctgggcaag ctgtgaaatc ttgctgcgcg gtctatgcgg gccacatccc
     2101 taactccggg tctgaaggcg ctcacgcctt ctcgagagct tgctcaatgt cggcgatcaa
     2161 gtcatcaacg tgttcgagtc cgagggagag gcgcacgtaa tcgggcgtaa ctccggtggc
     2221 gcgctgctcc tccaaggaga gttgttggtg agtggtggtg gccgggtgga tcgctaaact
     2281 cttggcgtca cccacgttcg cgacatgcga cagcaagttc aaacggtcga tgaagcggcg
     2341 gcccgcctcg gctccgcctt cgacgccaaa accgaggatc gcaccagcgc ccttgggtaa
     2401 gtacttccgg acgcgctctc gctccgagct ctcttcgaga ccggggtagt tcacccagcg
     2461 aacctttttg tgggtggcca aatagcgggc caccgccagt gcgttctccg aatgcttgat
     2521 cagccgcaga gggagcgtct cgagtccttg gaggaacaga aacgaattga aaggtgataa
     2581 agccgggccg agatcgcgga gcagcgtgac gcgggccttc acgatataag cgatgttgcc
     2641 cagcggcttg agcgcctcga cgaaattcag cccgtgatag ctcggctcgg gcccggctat
     2701 cagcggatgc ttgtcctgag tccactcgaa cttgccgctg tccacgatca cgccaccgat
     2761 cgacgtcccg tggccgccga tgaacttggt cgcggagtag actgcaatat ccaccccatg
     2821 attgaacggc cgcagtaggt acggcgagac ggtgttatcg agcacgaacg gtaccccgtt
     2881 ggcgtgtgca atcttcgcga tgccctcgag ctcggctaca tccagcttgg ggttgccgat
     2941 tgactccgca tacacagcct tgacgcgcgg ggtgatcgcc ttggccaaag cgtccaggtc
     3001 gttggacggt acgaagcgaa ccttgatgcc gagacgcggg aacgtgtagt ggaagaggtt
     3061 gtacgtgcct ccgtacaggt tgtccgccga aacaatctca tcgccggccc gagcgaggtt
     3121 cagaagactg agcgagattg cggattgccc acttgccacg gcgagcgccc cggcaccgcc
     3181 gtccagagcc gcgatgcggc gctcgaacac gtcggtggtg gggttcatca gacgcgtgta
     3241 gatccagcca aactccttca aagcaaaaag gttggccgcg tgctcggtgt ccttgaattg
     3301 ataggacgtg gtctggtgaa ttggaacggc gcgtgaacca gtggtggggt cggggtcttg
     3361 cccgccgtgc agcgccaggg tttcgggctt gaatgcaggg tgtggcatgg gctcgtctgt
     3421 cctgtaaagg agacggtagt ccactttcat caaatcgcaa gaccgcctcg gcgggagccg
     3481 cgcagaaaat gacgccactc agcgtatctt cgatacgctc aatttctagt aaactggagg
     3541 aagccgggat gcgcggctcc agccaaggtg agatggttga atgaagcgtg gcggccatct
     3601 ggtcgaagtt tcacctgacg tcacgagacg ttttcattgc aagccgacga ggctcccttc
     3661 aaagtccgcg agaagcccac aagtcccgtg gctccaagcg gctccgatgc gcaaatctcg
     3721 catcaggcga aacgccatcg ctcgccttcg cagacgcagc gctcccctct cgcgcagctc
     3781 cgctccgcga gccgcagttc ccccttggcc gcgcgcctcg cagcccaatc caggcgacaa
     3841 ctctgctagc ttagcggcac tggcgcgtgg gtcatggacc atctgacgca ttcgatacgt
     3901 ccgatacgga aaaatccctt cgagttctgc cgccgcgact cgctgccgtg ccgcttcgtg
     3961 tgctcgacgg aacgcgacca gctgcgtgag caagcgcacg cgggttgcct cgtctctgca
     4021 cgccgccgcc ggccgcacgt tgcgccgtgg ctccgagctc tgcggtgtat cgaactgctt
     4081 gtcatcgcac tgagccttgg ctgcgcgggg cgaactcatt ccgccgtgga tgccggcgct
     4141 gcgagccgcg gcaacgctga cgaggtggac ggaaccgtcg accgggagga gctgctgcaa
     4201 gagcttgcgt gtgccgccca ggagttcgtg cgcactaact cgcagggcgt gcgccactgg
     4261 caacttgtgc agcgcgtcct aaagtccggc gtactcatag ggttgaccaa ggggcagatc
     4321 gctgcacgat tgggccggtc taggcgctgc cctccgggga taggttatgc caagcaacag
     4381 gggtcttggg aatgctatcc tatcggtaga
//
LOCUS       DASTKU010000933        11261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1969471, whole genome shotgun sequence.
ACCESSION   DASTKU010000933 DASTKU010000000
VERSION     DASTKU010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11261
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969471"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFQ61_32990"
     CDS             complement(<1..261)
                     /locus_tag="VFQ61_32990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET9959369.1"
                     /translation="MKRRLARGFTLTELMVVVTLVGVLATIGVASFRKQVMASKSSEA
                     LSVVQALRGAEEAYRSENQLYLNVSTNGVWYPLDAQGSNYHGF"
     gene            complement(307..1731)
                     /locus_tag="VFQ61_32995"
     CDS             complement(307..1731)
                     /locus_tag="VFQ61_32995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9959370.1"
                     /translation="MTSIAPPPLKVLIVEDEPYLREMLGISFRREGYDVVLAPGAKSA
                     LEAIAQHPQPFPVVLTDLAMPDGSGLDVLAGAKSRSASTEVILITAHSSLENAIAAMR
                     LGAYDFVSKPFELIELTALVAKALEKQAIVVENLRLRARVEGPNARDLVGRSAALRKV
                     TDLIERVAPTRTTVLITGESGTGKERVARALHQMSDRADKPFLVVNCGALPDNLMESE
                     LFGHEKGAFTGAQSRHRGLFREADGGTILLDEVGELPAVLQVKLLRVLQERLVRPVGA
                     TQEVPVDVRVLAATNRTIEADVASGKFRGDLYYRLNVIRIELPPLRERREDVPVLAEL
                     FVRRFATEMGKDIVGFTPDALRALQTYPFPGNVRELENVIERAVALAGSRAIGLGDLP
                     PELSGVAGAPTPALLALPAEGTRLDDVLTEVERRLMLEALERTGGVRKRAAELLGITF
                     RSLRYRLKKHGLGDDNEEETPDPE"
     gene            1838..3568
                     /locus_tag="VFQ61_33000"
     CDS             1838..3568
                     /locus_tag="VFQ61_33000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959371.1"
                     /translation="MLRPIVSVPRPSPEPTAPPAAFVTSAAHDRRPWIDELLRGGRIP
                     LALGLMVAALYGCSSSDSRPTGPFTGGQGGTGGAAMVSLRFVGDGGALQLAPRDFRRV
                     TAAVDPPGAYTVRFFLLPETDDPGAISGVGDAAIDQSEVISDAEGVAIVTLTAPSQPA
                     RLRLRASTGSVSADLPVVVEQAGLATLKVTPKYLGRREVTGWTSSVHLQARCESIQTE
                     MGYPDAPLLLRTPPNAKIALEDVPAVSPLAVVVRGGALIDGCATLESAVPRGENAVEV
                     ALVDRPLAQPDQPLADNLLAFRLGSDGVALQTTLAATATQLQSAFRGYSSNDVTAVLN
                     AMEQSLDRDAGRLFGEAREAALWDEVLPSALGRSSGRRLSDAIGRWINVGSRVLQDQA
                     IEGLLDSKGLEVQRIAGASASDTHMQRVDTGDLEVDASDTLAMSTTLAWSSSSLADAV
                     ARPPALAETGVGTLGEALAQLLSCTTLARTLAELAEPEVQAWLEECDETCTEASCVTA
                     VDSMWTAALSSVDAEAQLQIAATAQLRLDATARVHSFEGTWVGLLSSGSHTTRLRGPF
                     SGTNESRLNP"
     gene            3815..4414
                     /locus_tag="VFQ61_33005"
     CDS             3815..4414
                     /locus_tag="VFQ61_33005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9959372.1"
                     /translation="MIDGAAPNLEAERALCERARAGDRVALAQLLRSHGPRLYRSVLL
                     PRLGSTAAAEDALSQTYVKVVERISQFSWQSVGFYPWLRSVGMHVAIDALRRRKRETL
                     FEPSDLEREIELAPEDAGDPKAVEEHDLARARARVEGLLDGLNPRYAMAIRLRVLEQR
                     PREEVAGILKVSVATFDVVLHRALAALKKAISAQEVAAP"
     gene            4441..5139
                     /locus_tag="VFQ61_33010"
     CDS             4441..5139
                     /locus_tag="VFQ61_33010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959373.1"
                     /translation="MKRERAEHDTLLGELAAELRAAHVPEELAPGRHEALLLAALGPA
                     GALAESRTESDAEAPASEEESAEAERLRVALEGRGTHPLAHIATLLQLGHAPPHVSEL
                     SLGRAERAAGLPRARITPLRWGAMGFATLAAAAGVVLWLQGVPTEASSEPSARWLRAR
                     SAASMFSVSTFSATMLSAAEVAGAELDGNRLDPKSPEPLNAEALDYTARVDRIFAARE
                     RDLRENWYRARGLP"
     gene            5136..5741
                     /locus_tag="VFQ61_33015"
     CDS             5136..5741
                     /locus_tag="VFQ61_33015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959374.1"
                     /translation="MRHRLLLWMCFMAACGSLLLGCARRRDDCSCFDEAGMRPVDPAL
                     LAFLSQARSAHRLADAAESRGDLPGAVRILEQWTEKPAPVGAPEVKEVQADTLARLAD
                     LESRRERFDRADASIERGLSIVLEPTYFRGRLYEVRGLIEERRAAAMKSRGDLEQAEA
                     ARQRALQALEMSMKIQSDVIEKSTRAHSKPVTTNPPADPKP"
     gene            5859..7517
                     /locus_tag="VFQ61_33020"
     CDS             5859..7517
                     /locus_tag="VFQ61_33020"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET9959375.1"
                     /translation="MTTELVRIPRLVAGVDIRALPLAPAEAFVLSRIDGVSSDAEIAL
                     ATALELAMVEQVLTHLSELGAIELQDQARPPKAAPVRRPRASTSGPWATEELIAPRAQ
                     TQARSRAPTELDEVVDLDEAKKRQILDFYQRLEAATYYELLNVDPSADRRAIKAKFYE
                     IINLYHPDRFYGKKLGSFKNKLERIFQCVSDAHDVLTRAEARAEYDAYLQAEQSTRVL
                     EEQLHDEEAYARELDAVNARIAAEAEGHPSQPVARESQPSAPLHISSAPPLSARPLST
                     PPVIQSSMPPGVSAVRNVSAPPPATGSLPPPTASSDPAPARGDPEARRRALARKLGLS
                     SPPPERRSVPAPAIPRDPMPRDPAQARDFAGQELKRRYEQRMLDARRRQIEEYTSRAD
                     AALAERKLVEAVNALRIAVSLAPQDPTLRQRLDDLQSEAGRELSDRYLEQARYEEREQ
                     RWAEAVRSYMRALSGKPNPRLHERTAHCLLAAQGDVKQAVEHARKAVLQVPNEPRFRV
                     TLGRAYYAANMRESAIGELERAASLAPTDDTIKDLLRRARRGEI"
     gene            7553..9376
                     /gene="dnaK"
                     /locus_tag="VFQ61_33025"
     CDS             7553..9376
                     /gene="dnaK"
                     /locus_tag="VFQ61_33025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET9959376.1"
                     /translation="MERVIGIDLGTTNSCVSILEGEQPVVIPNRGGYKTTPSVVAVTE
                     AGKRLVGHIAKRQAITNAENTVYAAKRLIGRKWNSPQVKNAILTSSYTIIEGPHADVR
                     IKLRDKTYSVPEISSMVLQEMKIIAEDYLGEPVKKAVITVPAYFNDNQRQATKDAGAI
                     AGLDVIRIINEPTAASLAYGYGKDFDRTVAVYDLGGGTFDVSILEIGATGVFKVISTT
                     GDTFLGGEDFDARIIDWLVSGFQQDHSIDLRQDRMALQRLRDAAEKAKCELSSVKETE
                     INLPFIISTGRNEALHLQRTLTRDTLEKLTDDLVERTVEICRQALEDARLHVDEIEDV
                     ILVGGMTRMPAVQTAVAGFFGREPNRSVHPDEVVAVGAAIQGAALTQDRHEMILLDVT
                     PHALGIMTFGSHFEELIPQNTTVPTSRAKIFTTSRDDQTAVKILVMQGESKRATENEL
                     LGEFILTGLRRAPKGHVEIEVTFEINTDGIVSVHAKDLETGQAQSIQVTATSGLTQDE
                     IKNMMSSAQDYLVERRSDEDFEGAKQEAETLMAEIERMFPEIERVVGTADFGRDAINK
                     ARSTLEKARAAIAAKDTAAVRLQVEQLQRTQRMFKGVVARG"
     gene            9428..10324
                     /locus_tag="VFQ61_33030"
     CDS             9428..10324
                     /locus_tag="VFQ61_33030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959377.1"
                     /translation="MSKPQAPAEPPRPAKKDEPGSASIHDVTWDDDTAPHLDFDERAF
                     DRVTAIPEMPPELFAKQVMEAAAAAETAIPQPATEPPPVSVPRAPSIPFELATSERAS
                     PIDVGPPSAPPHSVRQARALAARGAEAGGRDLLSDPGALSSKPAPSPRGMALDLDLNG
                     LEVSLDKGFARDEDPALRELADRHAMGDFSGALVLAEGVLETQPDHPEALRYAKNCRD
                     VLTSMYTARLGPLNQLVRVVIPTEQIRWLSLDHRAGFLLSLVDGISTLEEILDISGMP
                     RLDALRIMYSLREERVITLSPK"
     gene            10321..>11261
                     /locus_tag="VFQ61_33035"
     CDS             10321..>11261
                     /locus_tag="VFQ61_33035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HET9959378.1"
                     /translation="MTSDPEFDRRAPHPSSSKAISGARLWLELAAGFGLAGFGIWLGL
                     AHLQPPTRHSSNSLGAAGTLTNVGTPAEAAAPNTAKASAMASGAATELSRVPAVASYT
                     MQAKLNPTTHVVKGTQRIVWTNTSRAAVSSLYFHLYLNAFKNEQSLFLRSPFNASRGA
                     VRAQEFGYIDVKSMVAQEWPGQDLWLSRAAHSPGDPLDETDIEVPLPSPVAPGSPVTL
                     DVTFEARLPSVVERTGYSGRFHFVAQWFPKIARLESDGSFAHFAFHPQSEFYADFGRY
                     DVTLEVPEAFRLGATGRRIEQQVVTGTRRERYVAEAV"
BASE COUNT         2113 a         3639 c         3618 g         1891 t
ORIGIN      
        1 gaagccatgg taattcgagc cctgggcgtc gagcggatac caaacaccgt tggtcgagac
       61 gttcagatac agctggtttt cggagcggta cgcctcctcc gccccgcgca gcgcctgcac
      121 tacgctcaat gcttcggagg acttggacgc catcacctgc ttacgaaatg aagctacccc
      181 aatggtcgcc agcacgccga ccaaggtcac gaccaccatc agctcggtga gcgtgaaacc
      241 gcgagcgagg cgcctcttca ccgaacacat ccgaacgaca acgtagcacg ccgcattggg
      301 cacagctcac tccggatcgg gagtttcctc ctcgttgtcg tcccccaatc cgtgcttctt
      361 cagacggtaa cgcagagaac gaaaggtaat acccaagagc tccgcggcgc gtttgcgcac
      421 acctcccgtc cgttcgagcg cctccaacat cagtcggcgt tcgacttccg taagcacgtc
      481 gtccaggcgt gtgccttcgg ccgggagcgc cagcagcgcc ggtgtgggcg ctcccgccac
      541 tccactcaac tcgggcggca ggtcgccgag gccaatcgcg cgcgagccgg cgagtgccac
      601 ggcccgctca atcacgttct cgagctcgcg cacgttgcct ggaaatgggt aggtctgcag
      661 cgcccgcagt gcatctggcg tgaaaccgac gatgtcctta cccatctcgg tcgcaaagcg
      721 gcgcacgaac agctctgcaa gcaccggaac gtcttcacgc cgttctcgca gcgggggcag
      781 ctcgatgcga atcacgttca gacggtagta aaggtcgcca cgaaacttgc ccgaggccac
      841 gtccgcctcg atggttcgat tggtggccgc caacacgcgc acgtccaccg ggacttcttg
      901 agtcgcgccg acggggcgca ccaagcgctc ctgcagcacc cgcaagagct tgacctgcag
      961 taccgcgggc aactccccca cctcgtcgag cagaatcgtg ccgccatccg cttcgcggaa
     1021 taggccgcga tggcgtgact gagcgccggt gaatgcgccc ttttcgtgcc cgaacagctc
     1081 actttccatc aagttgtcgg gtaacgcgcc gcaattcacc accaaaaagg gtttatccgc
     1141 ccgatcggac atttggtgca gggcgcgcgc cacacgctcc ttaccagtgc cactctcccc
     1201 agtaattaga accgtggtgc gagtgggtgc gactcgttcg attaggtccg ttaccttgcg
     1261 taaggcggcg ctgcgcccca ccaagtcacg cgcattgggt ccctccaccc gagcccgcaa
     1321 ccgtaggttt tcgacgacga tggcttgctt ttcgagcgcc ttcgccacca acgccgtcag
     1381 ctcgatgagc tcgaacggct tggaaacgaa gtcataagcg ccgagtcgca tcgccgcgat
     1441 ggcgttctcg agtgaggagt gggcggtaat gaggattacc tccgtcgaag cgctgcgtga
     1501 cttcgcgccc gccagcacgt ccaaccccga accatccggc atggccaagt ccgttaggac
     1561 taccggaaag ggttgcggat gctgcgcgat ggcttccagg gccgacttgg cgccgggcgc
     1621 caagacaacg tcgtagccct cgcggcgaaa cgagatgccc agcatttcac gcaagtaggg
     1681 ctcgtcctcg acgatcagga ctttcaaagg gggaggcgcg atcgaggtca cttcggcagg
     1741 ctccagggtt ctcgaaaaga ctggactagt ccagcccagg agcagtgcat aagcttattc
     1801 ccgtcggatc gcagctcgcg ctatgctctc ggcacccgtg ctccgcccga tcgtcagtgt
     1861 gccccggccg tcacccgaac caactgctcc gccagccgct ttcgtcactt cggctgcgca
     1921 cgatcggaga ccatggattg acgagctgct gaggggaggc cgcatcccgt tggcactcgg
     1981 gctcatggtc gcggcgctct acggctgcag ttctagcgat tcccgcccta ccgggccctt
     2041 cacgggtggg caggggggca ccggcggcgc tgcgatggtg agcctgcgtt tcgtgggcga
     2101 tgggggtgcg ctgcagttgg ctccgcgcga ttttcgtcgc gtcactgcgg cggtcgatcc
     2161 tccgggcgca tacacggtgc gttttttctt gttgcccgag acggatgacc ccggcgcgat
     2221 atcgggggtc ggggacgccg ccatcgacca gagtgaggtg atcagcgacg ccgagggggt
     2281 cgccatcgtt acactcaccg ccccgagcca accggccagg ctccgtttgc gggcgagcac
     2341 gggcagcgtg tccgcggatt taccagtagt cgtcgagcaa gctggtctcg cgacgctcaa
     2401 ggtcacgccg aagtatttgg ggcggcgcga agtgaccggc tggaccagca gcgtccacct
     2461 ccaagcgcgc tgcgaatcca ttcaaaccga gatgggttat ccggatgcgc cgctgctact
     2521 tcggaccccg ccgaacgcca agatcgcgct cgaggacgtg cccgcagtgt cgccactcgc
     2581 ggtcgtggtt cgcggaggcg cgctgataga cgggtgcgcg accttggaaa gtgcggtgcc
     2641 acggggggaa aacgcagtgg aggtcgcgct ggtggaccgt ccgctcgccc aacccgatca
     2701 acccctcgca gataatttgc tcgcctttcg cttgggaagc gacggggttg ccctccaaac
     2761 cacgctcgcc gcgacggcca cgcaacttca gtcggcgttc cgaggctact ccagcaacga
     2821 cgtgacggcg gtgttgaacg ctatggagca gagcctggac cgagacgcgg gtaggctgtt
     2881 cggagaggcc cgagaagccg cgctctggga cgaagtgctg ccaagcgcgc tggggcggtc
     2941 ctcggggcgc cggctcagcg acgcgatcgg gcgctggatc aatgtcgggt cacgcgtgct
     3001 gcaagaccag gcgatcgaag ggctcctcga cagcaagggg ctcgaggtgc agcgcattgc
     3061 gggtgcctcg gcgtcggaca cgcacatgca gcgcgtggat acgggagatc tcgaagtcga
     3121 cgcctccgat accttggcca tgagcaccac gctcgcctgg tcgagcagca gcttggcgga
     3181 cgcggtggcg cgtccacccg cattggccga aaccggcgtg ggtacgctcg gcgaggcgct
     3241 cgctcaactg ctctcgtgca cgacactggc gcgtacgctg gccgagctcg ccgagccgga
     3301 ggtgcaagcc tggctcgagg agtgcgatga aacctgcacc gaagcgagct gcgtcacggc
     3361 ggtcgatagt atgtggacgg cagcactcag tagcgtggac gccgaagcgc agctgcaaat
     3421 cgccgctacg gcgcagctgc gtctggacgc gacggcgcgg gtgcacagct tcgaagggac
     3481 gtgggtgggc ctgctgtcgt cgggctcgca cacgactcga ctacgagggc cgttctcggg
     3541 caccaatgag tcccgtctga acccttgacg gcgtcgccct acagggaatc cccgcgtggt
     3601 ggaggctcgc cgagcgcggg gccgtggaat ttgggcttgg agcgtaagcc cgcatgcgtc
     3661 gccgcgttcc tcgtgagtgc ttgcctccac gctcgccatc ctcactccct ggacgtcggg
     3721 ggcaaccctc gggccctctc ttcttcgagt cgaatgttct tattccgacg cggtggtctc
     3781 cagctcgcgc gcctcacggg ctagactggc gcccgtgatc gacggcgctg cccccaatct
     3841 cgaagccgag cgagcgctct gcgaacgagc gagagccggg gaccgagtgg cgctggcgca
     3901 gcttctacga agccacggtc cacggttgta tcgcagtgtc cttttgccgc ggctgggcag
     3961 caccgcggcc gcggaagacg cactgtccca gacttacgtc aaagtcgtgg agcggattag
     4021 ccagttttcg tggcaaagcg ttggcttcta cccctggctg cgcagcgtcg gaatgcacgt
     4081 cgcgatcgat gcgctccgcc gcaggaaacg cgagacactg ttcgagccct cggacctgga
     4141 gcgcgagatc gagctcgcgc cagaggacgc tggggatccc aaagcggtcg aggagcacga
     4201 cttggcgcgc gcgcgtgccc gcgtggaagg cctgctcgat gggctgaatc ctcgatacgc
     4261 tatggcgatt cgtctgcgcg ttctcgagca acgtccgcga gaggaggtcg cgggcattct
     4321 caaggtcagc gtggcgacgt tcgacgtggt actgcatcgg gccctcgcgg ctctgaaaaa
     4381 ggccatctca gcgcaggagg tcgcggcgcc gtgacggatg aagaagatcg atccccggga
     4441 atgaagagag agcgcgcgga acacgacacg ctgttaggtg agttggcagc cgagctgcgc
     4501 gcggcccacg tgcccgagga gctggctcca ggtcgccacg aggcgctgct tctcgccgca
     4561 ctaggccctg ctggtgcgct cgcagagagt aggaccgaaa gtgacgcaga ggctccggcg
     4621 tccgaggaag aaagcgcgga ggccgaacgg ctgcgtgtag ctttggaagg acgagggact
     4681 caccctttgg cgcacatcgc tacgctgttg cagcttggtc atgctcctcc gcatgtcagt
     4741 gagctatccc taggccgagc cgagcgcgct gctggactac cgcgcgcgcg catcactccg
     4801 ctccgttggg gcgcgatggg atttgccacg ctcgccgcgg cggctggggt tgtgctctgg
     4861 ctccaaggtg tgccgaccga agcctcgtca gagccaagcg cgcggtggct gcgggcacgc
     4921 tccgccgcaa gcatgttttc cgtgagtacg ttttccgcga ctatgctctc tgcagccgag
     4981 gttgcgggag cagagctcga tgggaatcgc ctcgatccga agtcacccga gccgctgaat
     5041 gcggaggcgt tggactacac ggcgcgcgta gaccgcattt ttgcggctcg ggaacgagat
     5101 ctgcgggaaa attggtaccg ggcgcggggg ctgccatgag gcatcgcctg ctgctttgga
     5161 tgtgctttat ggccgcgtgc ggatccctgc ttctcggctg cgcacgtcgc cgcgacgact
     5221 gcagctgctt cgacgaagct gggatgcgtc ccgtcgatcc cgccctgctg gcgttcctga
     5281 gccaggcccg ctcggcacat cgattggcgg atgccgcaga gagtcgcggc gacctgcccg
     5341 gcgccgttcg aattttggag caatggacgg agaagcctgc acccgtcgga gccccagaag
     5401 tgaaggaggt acaggcggac acgctggcgc gccttgccga tctcgaaagc cggcgtgagc
     5461 ggttcgatcg cgctgacgcc tcgatcgagc gcggcctctc tatcgtactc gaacccacct
     5521 actttcgagg acgattgtac gaggtgcggg gactcatcga agagcgcaga gcggccgcta
     5581 tgaaaagtcg tggtgacctc gagcaggcgg aggccgcgcg ccagagggcc cttcaagcac
     5641 tggagatgtc gatgaagatc cagagcgatg tgatcgagaa gagcacccgt gcgcactcca
     5701 aacccgtcac cacaaaccca ccagccgacc cgaaaccgtg aacgttgcag gtcaaagccg
     5761 ccgccgcata acataaaact tcgcgtcggc gatttccccg ccggcgcagc tcgagctgtc
     5821 cgtttgcgta ccaaaagctc gggcggagtg cgttctgtgt gaccaccgaa ctcgtccgaa
     5881 tcccacgact cgtcgccggg gtcgatattc gcgccttgcc tctcgcgccg gcagaggcgt
     5941 tcgtgctgtc acgcatagat ggtgtgagtt cggacgcgga aatcgcactc gctaccgcgc
     6001 tcgagctcgc catggtggag caagtgctca cccatctcag cgagcttggt gcaatcgaat
     6061 tgcaggatca ggcgcgcccg cccaaggccg ctccagtgcg gcgcccgcgt gcgtcgactt
     6121 cggggccatg ggctaccgag gagctgatcg ctccccgcgc tcagacgcaa gcgcggtccc
     6181 gagcgcccac ggagctcgac gaagtcgtcg acttggacga ggcgaagaag cggcagattc
     6241 tggacttcta tcaacggctc gaagcagcca cgtactacga gctgttgaac gtggacccca
     6301 gcgccgatcg gcgcgcgatc aaggccaaat tttacgagat catcaacctt tatcaccccg
     6361 atcggtttta cggaaagaag ctcggcagct tcaaaaacaa gctcgaacgc atcttccaat
     6421 gcgtgagcga cgcgcatgac gtgctgacgc gggccgaagc gcgcgcggaa tacgacgcct
     6481 acctgcaggc cgagcagagc acccgtgtac tcgaggagca gcttcacgac gaagaagcct
     6541 atgcgcgaga gctcgatgcg gtaaacgcgc gcatcgcggc ggaggccgaa ggccacccga
     6601 gccagcccgt cgcacgcgag agtcaaccct cggcgccgct tcacatcagc agcgctcccc
     6661 cgctctcggc tcgcccgctc tctacgcccc cagtgattca gagctccatg ccgcccggtg
     6721 tgtcggccgt gcgcaacgtc tcggccccac cacccgccac cggatccctt ccgccaccta
     6781 cagccagcag cgatcccgca cctgcgcgcg gagatcccga agcgcggcgc cgcgctttgg
     6841 cgcgcaagct cggcttgtcc tcgcctccac cagagcgccg ttcggtgcct gcacctgcga
     6901 ttccccgcga tccgatgccc cgcgatccgg cccaggcgcg ggatttcgcc gggcaggagc
     6961 tgaagcgccg ttacgaacag cgaatgttgg atgcccgacg gcggcagatc gaggaataca
     7021 cctcacgcgc cgacgcggct ctggccgagc gcaagctcgt agaggccgtg aatgccctgc
     7081 gcatcgccgt gtcgctcgcg ccccaagacc cgaccctacg acagcggctc gatgacctcc
     7141 aaagcgaggc cggacgcgaa ctctcggaca ggtacctgga gcaagcgcgc tacgaggagc
     7201 gtgagcagcg ctgggcggag gcggtgcgct cctacatgcg cgccttgagc ggaaagccga
     7261 atccgcgcct tcacgagcgc acggcgcatt gtttgctggc cgctcagggg gatgtgaaac
     7321 aagcggtcga acacgcgcga aaagcggtcc tgcaggttcc gaacgagccc cgattccggg
     7381 taactttggg tcgcgcgtat tacgccgcga acatgcggga gagcgcgatc ggcgaactgg
     7441 aacgcgccgc gtcactcgcc ccgaccgatg atacaatcaa ggacctattg cgccgcgccc
     7501 gccgcggcga aatttgacga accccttcaa gaaagcggat gagagtaagg atatggagcg
     7561 cgtcatcggc atcgacctcg gaacgaccaa ctcgtgcgtg tcgattctcg agggggagca
     7621 gcccgtcgtc attccgaacc gcggcgggta caagaccacg cccagcgtgg tggcggtcac
     7681 cgaggcgggc aagcgcctgg tcgggcacat cgccaagcgc caagccatca ccaacgcgga
     7741 aaatacagtt tacgccgcca agcggttgat tggccgcaag tggaactccc cgcaagtcaa
     7801 gaacgcaatc ctgacctcga gctacacgat catcgaaggg ccgcatgccg acgtgcggat
     7861 caagctccga gacaagacgt actcggtgcc cgagatcagc tccatggtgc tgcaggagat
     7921 gaagattatc gccgaggatt acctaggcga accggtgaag aaggcggtca tcacggtgcc
     7981 cgcctacttc aacgacaatc aacggcaggc caccaaagat gcgggcgcca tcgcgggact
     8041 cgatgtcatc cgtatcatca acgagcccac ggcggcttcg ttggcgtacg gctacggcaa
     8101 ggacttcgac cgcaccgtcg ccgtctacga tctcgggggc ggcaccttcg acgtgagcat
     8161 cctggagatc ggcgcgaccg gcgtgttcaa ggtgattagc accaccggcg atacgttcct
     8221 gggcggagag gacttcgatg cccgaatcat cgactggtta gtgagcgggt tccagcaaga
     8281 tcactcgatt gatttgcgac aagatcgcat ggctctgcag cgtctgcgcg atgctgccga
     8341 gaaggccaag tgcgagctct cgagcgtcaa agagaccgag atcaacttgc ccttcatcat
     8401 ctccacgggc cgcaacgaag cgctgcacct ccagcgcacg ttgacgcgtg acaccttgga
     8461 gaagctgacc gacgatctgg tggagcgcac ggtggagatc tgtcggcagg ccttggagga
     8521 tgcgcgactg cacgtcgacg agatcgaaga cgtgattttg gtgggcggga tgacgcgcat
     8581 gcccgcagta caaacggcgg tggcgggctt ctttggccgc gagccgaacc gcagcgtaca
     8641 ccccgacgaa gtggtggcgg tcggcgccgc cattcagggc gccgccctaa cgcaggatcg
     8701 tcacgagatg atcctgctcg atgtgacccc gcacgcgctc gggatcatga ccttcggcag
     8761 ccacttcgag gagctgatcc cgcaaaacac cacggttccg actagccgcg cgaagatttt
     8821 cacgaccagc cgcgacgatc aaacggcggt gaagatcttg gtcatgcagg gcgagagcaa
     8881 gcgcgcgacg gagaacgagc tgctcggcga gttcatcttg acgggcttgc gtcgcgcgcc
     8941 caagggccac gtggagatcg aggtgacctt cgagatcaac acggatggca tcgtcagcgt
     9001 tcacgccaag gacctcgaga ccggtcaagc ccaatcgatc caggtgaccg ccacgagcgg
     9061 gctcacgcag gacgagatca agaacatgat gtcgtcggcg caggattatc tggtggagcg
     9121 gcgctcggac gaagacttcg agggcgccaa gcaagaagcc gagacgctga tggccgagat
     9181 cgagcgtatg ttcccagaga tcgagcgcgt cgtgggcacc gctgatttcg ggcgggatgc
     9241 catcaacaag gcccggagca cgctcgagaa agcgcgcgcc gccatcgcgg ccaaagacac
     9301 ggcggcggtc cgactgcagg tcgaacagct gcagcgaacc cagcgcatgt tcaagggcgt
     9361 cgtggcgcgc ggctgaactg tttcttcatg agcaagcccc gttcttcatg agcaagcctc
     9421 gtttcccatg agcaagcctc aggcacccgc tgagccgccc cgaccggcca agaaagacga
     9481 gcccggctcg gcgtcgatcc atgacgtgac ttgggacgat gacacggcac ctcacctgga
     9541 cttcgacgaa cgcgcgttcg atcgggtaac cgccatcccc gagatgccgc cggagctatt
     9601 cgcgaagcag gtgatggaag ccgcggccgc ggccgagaca gccattcctc aacccgcaac
     9661 cgagccccct ccggtgtccg ttccacgggc gccttcgatc cccttcgagc tggcgacgtc
     9721 cgagcgcgcg agccccatcg acgttgggcc gcccagcgct cctcctcaca gcgttcgcca
     9781 agcccgcgca ttggcggcgc ggggagcaga ggctggaggc cgggatttgc tgtccgatcc
     9841 aggagccttg agttccaaac ctgcaccgag cccgcgggga atggcgctcg atctcgactt
     9901 gaatggcctc gaggtcagcc tcgacaaagg cttcgcgcgc gatgaagatc ccgcgcttcg
     9961 tgagttggcg gaccgacacg cgatggggga tttctccgga gcgctggtgc tcgcggaagg
    10021 tgtgctcgag acgcagccgg atcaccccga ggccctacgc tacgccaaga attgccgcga
    10081 cgtgctgacg agcatgtata cggcgcggct cgggcccttg aaccagctcg tgcgggtcgt
    10141 aatcccgacc gagcagattc gctggctttc gctcgaccac cgagcgggtt tcttgttgtc
    10201 tttggtggac ggaatctcga cgctcgaaga aatcttggac atcagcggga tgccacggct
    10261 cgacgctctg agaataatgt attccctacg tgaagagcgc gtgattaccc tgtctccgaa
    10321 gtgacgtcag atcccgagtt cgaccgccgc gcgcctcatc cctcgtcatc gaaggcaatt
    10381 tcgggcgcca ggctttggct cgagctcgcc gcaggctttg ggctggctgg gttcgggata
    10441 tggctcggtt tggctcacct ccagccgccg actcgtcatt catcgaactc gttgggcgcg
    10501 gcgggaaccc tgacgaacgt cgggaccccg gctgaagcgg cggccccgaa caccgcgaaa
    10561 gcaagcgcaa tggcgagcgg tgcggccacc gagctgagcc gcgtaccggc cgtggcctcc
    10621 tacacgatgc aggccaagct caaccccacg acccacgtgg tcaagggtac tcagcgcatc
    10681 gtctggacga acacgtcgcg ggcagccgtg tcgtcgctct acttccactt gtatttgaac
    10741 gccttcaaaa acgaacaaag cctgtttttg cggtcgccat tcaacgccag ccgcggcgcg
    10801 gtgcgggctc aagagttcgg gtacatcgac gtcaaatcca tggtcgccca ggaatggccc
    10861 ggccaagact tgtggctgtc gcgggccgct catagcccgg gagacccgct cgacgaaacc
    10921 gacatcgagg tcccactgcc gagcccggtt gcgccgggca gcccagtgac gctcgacgtg
    10981 accttcgagg cgcgccttcc aagcgtggtc gagcgaactg gatattcggg gcgcttccat
    11041 ttcgttgcgc agtggttccc caagatcgcg cggctcgaga gcgacggctc gttcgcgcac
    11101 ttcgcgtttc acccacaatc cgagttctac gcggacttcg gccgctacga cgttacgctc
    11161 gaggtcccgg aggctttccg cttgggagcc acagggcgac gcatcgagca gcaagttgtc
    11221 acggggaccc ggcgcgaacg ctacgtcgca gaagcggtgc a
//
LOCUS       DASTKU010000934         4849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1744412, whole genome shotgun sequence.
ACCESSION   DASTKU010000934 DASTKU010000000
VERSION     DASTKU010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744412"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /locus_tag="VFQ61_33040"
     CDS             <1..1489
                     /locus_tag="VFQ61_33040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959379.1"
                     /translation="GKPTLTMPHLEVGDYIETERIESQPGDGRRGAQYMGPRWYFREE
                     NIAYARSEFVVISPKSKPLAIETRNDVSAPEVKEDGAIIARRWRVDLSPAAPVEPFGA
                     PVAEFMPSVQVGWGVNLPRTLEAMGDASADLTPVDPRVARIARRIVEKVPTVQREERA
                     RVLYRWLVTNVQEGEESDGRRVVIGQNGNLWRGYIALCRAVGLDVQYAVTQNRLTLPP
                     TGPFSEASQFTAPLMKVVTEKGDLWLSLGSKYAPFGYVPSELRGMPAYILAESGPKQT
                     RVPETGVPDRVTYEGDVDVQTDGSANVDLAQTMQGKYGMALRNALSSMSEQQIHSLIE
                     TRLIGNSLRGAKLVSYELVNLADADKPLTIRSRSRVPSFAQVAGSVLLVTPPFGPRVG
                     QLAALPVRQTPLLIADSTDQSIRLHIKLPKGTALVAEPAPRELSNNGFRVRIQDHREG
                     EDLVLERSIYLPAGRVQIKEYAEFLKFARSADDALSSSLRFRLKL"
     gene            complement(1571..2290)
                     /locus_tag="VFQ61_33045"
     CDS             complement(1571..2290)
                     /locus_tag="VFQ61_33045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959380.1"
                     /translation="MFVRPFAIIGKAGLAATLAILVTPAIAQAQSTSTAVPSSTPAPA
                     THTTGGNTAGPTATSPRPGDDLSTNTGARTAPAEALPVGTREALAKGDAAALARDYAG
                     AISAYQEAIRSSPQSALSHYRMGQAQVLAGQQKEAELSYAAAVRFSGKNLNFRRKVLF
                     CLADLAERQKNLALALERYSALASEPVSGEPEMPFASSAIERKKRLLEIQQLTADSAQ
                     VKARIEKRLKEVEAAGATKAR"
     gene            complement(2393..2845)
                     /locus_tag="VFQ61_33050"
     CDS             complement(2393..2845)
                     /locus_tag="VFQ61_33050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959381.1"
                     /translation="MNQKRGCLSLLLLATVAGTTVSCQRQASRGPDKVLVAGQRLQGT
                     WVLVSFKPEKPLEAPLQGLLDAQMRMLRISFDNSSYKAVGVGLNVQGRFQVLSAEGDL
                     FSGTMYDPTGVGYRVSGQFDGQQFHFASYDAPWRGTGLMERTAAAPTQ"
     gene            3109..3837
                     /locus_tag="VFQ61_33055"
     CDS             3109..3837
                     /locus_tag="VFQ61_33055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET9959382.1"
                     /translation="MGIAQNLELVRSRLEQAARRANRNPAEVRLVAVSKLHPAASIRE
                     AYAAGQRDFGENYVQELVGKARELADLDGLRWHLIGHLQRNKAKYVAPFVSLLHTVDS
                     LSLAGELAKRSAAAAERKGQPLPVLVEVSIAGEEQKSGAALSELPALLEAIDGEPSLT
                     LRGLMCVPPATATGDAARPYFETLRRLRDEYGGTARLPELSMGMSSDLEAAVAAGATW
                     VRVGTAIFGERPVNNPREPEPGGG"
     gene            3887..>4849
                     /locus_tag="VFQ61_33060"
     CDS             3887..>4849
                     /locus_tag="VFQ61_33060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9959383.1"
                     /translation="MARPPTRGRKDSATSQEPTLLSHAAEQGSLGNAPKPLADRMRPR
                     TLSEVLGQVHLLGPGKLLGEAIRGDRVPSFILWGPPGSGKTTLANVVAHVTRTEFVPF
                     SAVLGGLPELRQIIEAAKERRRYQGRGTTLFVDEIHRFNKGQQDAFLPHIESGTLTLI
                     GATTENPSFAINAALLSRCRVFRLEPLEPEQLVALLDRALRDPERGLGELNLQAQPEA
                     LRAIAESARGDARRALGLLEVAAGLAAASAGGRPGELDLSVVERAAEERTLLYDKSAE
                     EHYNVSSALIKSMRGSDPDAALYWLLRMLDAGDDPLFLSRRLMIF"
BASE COUNT          843 a         1606 c         1550 g          850 t
ORIGIN      
        1 gggcaagccc acgctgacga tgccccacct cgaagtcggg gattacatcg agaccgagcg
       61 catcgagtcc cagccgggtg acggccggcg cggcgcgcag tacatgggcc cgcgctggta
      121 cttccgcgag gagaacatcg cctacgctcg cagcgagttc gtcgtgatct cgccgaagag
      181 caagccgctc gcgatcgaga cccgtaatga cgtgagcgcg ccggaggtga aggaagacgg
      241 cgcgattatc gcgcgtcgct ggcgcgtcga tttgagcccg gcggcaccgg tcgagccgtt
      301 cggggcgccc gtcgcggagt tcatgcccag cgtgcaagtg ggctggggcg tgaatctccc
      361 gcgtacgctg gaggccatgg gcgacgcctc tgccgacttg acccctgtcg atccgcgcgt
      421 ggctcgcatc gctcgccgca tcgtggagaa ggtgccaacc gtgcagcgag aggaacgagc
      481 gagagtgctc tatcgctggc tggtcaccaa cgttcaggaa ggtgaggaga gcgacggccg
      541 ccgcgtggtg atcgggcaga acggcaacct gtggcgcgga tacatcgcgc tgtgtcgcgc
      601 ggtgggtctc gatgtgcagt acgcggtgac gcagaacagg ctcacgcttc cgcctacggg
      661 gccgttctcg gaggcctctc agttcacggc tcccctcatg aaagtcgtga ccgagaaggg
      721 cgacctgtgg ctctcgctcg gcagcaagta cgcgccgttc gggtatgtgc cctcggagtt
      781 gcgcggcatg cccgcgtaca ttttggctga atcggggccc aagcagaccc gcgtccccga
      841 aaccggtgtg ccagaccggg tgacgtacga gggcgacgtg gacgtgcaaa cggacggctc
      901 cgcgaacgtc gatctcgcgc aaaccatgca gggcaaatac ggcatggccc tgcgcaacgc
      961 gctgtcctcg atgtccgagc agcagatcca cagcttgatc gagacgcgcc tgatcgggaa
     1021 ctcgttgcgc ggggcgaagc tcgtctctta cgagctggtc aatctcgcgg acgcggacaa
     1081 accgctcacg atccggtcgc gatcgcgggt gccctcgttc gcgcaggtgg ccggcagcgt
     1141 gctgctcgtc acgcccccgt tcgggccgcg cgtgggccag cttgcggcgc tgcccgtgcg
     1201 tcagacaccg cttctgatcg cagactcgac cgatcagtcg atccgcttgc acatcaagct
     1261 gccgaagggc accgcactcg tcgcggagcc ggcgccccgc gagctctcga acaacggctt
     1321 ccgcgtgcgc atccaggatc atcgcgaagg cgaggatcta gtgctggagc gcagcatcta
     1381 tctgcctgcc gggcgcgtgc agatcaaaga gtacgccgag ttcttgaagt tcgcgcgcag
     1441 cgccgacgat gcgctctcgt cgagcttgcg cttccggctc aagctttgaa cgttcgaggc
     1501 agtctcgacg ctccgcccga aaccgagcgg agcgtcgggc gactggcacg gacggtcgcc
     1561 gtgcagccgt tcagcgcgcc ttcgtggcgc ccgcggcctc tacctctttg aggcgctttt
     1621 cgatgcgtgc cttcacttgc gctgagtcgg ccgtgagctg ctgaatctcg agcaggcgct
     1681 tcttccgttc aatcgcgctg ctcgcgaacg gcatctctgg ctccccgctg acgggctcgc
     1741 tcgccaatgc cgaatagcgc tcgagcgcca acgccaagtt cttttggcgt tccgccagat
     1801 ccgccaaaca gaacagaacc ttgcgacgaa aattcagatt cttgcccgag aagcgcacgg
     1861 ccgcggcgta cgagagctcg gcctctttct gttgtccggc gagcacttgg gcttgaccca
     1921 ttcgatagtg agatagtgca ctctgggggc tgctgcgaat cgcttcctgg tacgcggaga
     1981 tggcccccgc gtagtctcgc gccaacgccg ctgcgtctcc cttggccaac gcctctcgcg
     2041 tgcctaccgg caatgcttca gcgggagccg tacgagcacc cgtgttcgtg ctcaaatcat
     2101 cacctggtcg tggagatgtg gccgttggtc ccgcggtatt gccacctgtc gtatgggtgg
     2161 cgggagcagg ggtactgcta ggaaccgcgg tgctcgtgct ttgcgcttgg gcgatcgccg
     2221 gggtcaccaa aatcgcgagc gtcgcagcca atccagcctt cccgatgatc gcaaaagggc
     2281 ggacgaacat gcgagcaggc tatcatgaag ccaacacccg cgtcgtggct cgcggccctg
     2341 gatccgcttc gtcagtcgat tggggaccaa cgtcgagctg ggctggttcg gcctactgag
     2401 ttggggcagc cgctgttcgc tccatgaggc cggtgccgcg ccacggcgca tcgtacgagg
     2461 cgaagtggaa ctgctgcccg tcgaactgcc cggagacgcg gtaacccacc ccggtcggat
     2521 cgtacatcgt gccggagaac aggtcccctt ctgcgctcag cacctggaac ctgccctgca
     2581 cgttgagccc cacccccacc gccttgtagc tcgagttgtc gaacgagatg cgcagcatcc
     2641 gcatttgtgc atccaacaac ccctgcagcg gagcctccag aggcttctcc ggtttgaaac
     2701 tcaccagcac ccaagtgcct tgcagacgct gtccggcaac caacaccttg tcgggcccac
     2761 ggctcgcttg gcgctgacag ctcacggtgg tgcctgcgac agtggcaagc aggagaaggg
     2821 acaggcatcc acgcttctgg ttcatgttca gcaccgagac accgaagacc tttccgccag
     2881 aggttgtacg gggttcacag caggaccgca aaccccgcgg ccgggccagc cgaaggtctc
     2941 ctggccggac gagtcccacg gtcgtcacaa acttatccac agaactgtgg gtcccttgtg
     3001 gatgacttgc aggcgaacct gtgaacaggg cggcttggcc acaagaatgc gggggagcca
     3061 catttcggcc ccactcgccc agcgtgctag tcagggccca cacacaccat gggcatcgcg
     3121 cagaacctgg agctcgttcg cagtcgtctc gagcaggccg ctcgccgcgc caaccgcaat
     3181 ccagcggaag ttcgcctggt cgcggtttcg aagctgcacc cagcggcctc gattcgcgag
     3241 gcttatgccg cggggcagcg ggatttcggt gaaaactacg tgcaagagct ggtcggcaag
     3301 gcgcgtgaac tcgccgacct cgatgggctg cgctggcatt tgattggtca tctgcagcgc
     3361 aacaaggcca agtacgtagc ccccttcgtt tctctactgc acaccgtaga ttcgctatcg
     3421 ctcgcgggcg agttggcgaa gcgcagcgcg gcggcggccg aacgaaaggg ccagccgctg
     3481 cccgtgctcg tcgaggtgag catcgcgggt gaagagcaga agagtggggc cgccctcagc
     3541 gagctgcctg cgttgttgga ggccatcgac ggcgagccga gcctcacgtt gcgcggtttg
     3601 atgtgcgtgc cgccggccac cgccaccggt gatgccgccc gtccgtactt cgaaacgctc
     3661 cgtcgtttac gcgatgagta cggcggtacg gcgcgattgc ccgagctgag catgggcatg
     3721 tcttctgacc tcgaggccgc cgtggccgca ggcgccacct gggtgcgcgt cggaaccgcc
     3781 atctttggcg aacggcccgt gaacaaccca cgtgagcccg aaccgggcgg cggttgaaaa
     3841 atccgaaagg ccttccaagc tccgagccgt ctcccgaaca ctgatcatgg ccaggcctcc
     3901 cacgcgtggg cgtaaagact ctgcgacgtc ccaagagccc acgttgctca gccacgccgc
     3961 cgagcagggc tcactcggta acgcgcccaa gccgctcgcc gatcgcatgc ggccgcgaac
     4021 cctgtccgag gtgcttggcc aggtgcacct gcttggcccg ggcaagcttc tcggcgaagc
     4081 gatccgcggt gatcgagtgc cctcgttcat cctctggggc ccacccggct ccggcaagac
     4141 caccctcgcc aacgtggtgg ctcacgttac tcgtacggag ttcgtgccct tcagcgccgt
     4201 gctcggaggc ctgcccgagc tccggcagat catcgaagcc gccaaggagc ggcgtcgtta
     4261 tcaaggtcgc ggcaccacct tattcgtgga cgagatccat cgcttcaaca agggtcagca
     4321 ggacgccttc ttgccgcaca tcgagagcgg cactctcacg ttgatcgggg ccaccaccga
     4381 gaacccgtcg tttgccatca atgccgcgct gctctcgcgc tgtcgagtat ttcggctcga
     4441 gccactcgag cccgagcagc tcgttgcgct cttggatcgc gcactcagag acccagaacg
     4501 cggccttggg gaattgaatt tgcaagccca acccgaggcg ctccgtgcga tcgcggagtc
     4561 cgcgcgcggg gacgcccggc gtgcactcgg cttactcgaa gtcgccgcgg gcttggccgc
     4621 cgccagcgcc ggggggcgac cgggagaact cgatttatcg gtcgtcgaac gtgccgccga
     4681 agagcgcact ctgctctacg acaagagcgc cgaggagcac tacaacgtct ccagcgcgct
     4741 catcaaatcc atgcgcggca gcgatcccga cgcggccttg tactggctcc tgcgcatgct
     4801 ggacgccgga gacgacccac tatttctgtc tcgacgcctg atgatcttc
//
LOCUS       DASTKU010000935         6183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_850603, whole genome shotgun sequence.
ACCESSION   DASTKU010000935 DASTKU010000000
VERSION     DASTKU010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6183
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850603"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..4031
                     /locus_tag="VFQ61_33065"
     CDS             120..4031
                     /locus_tag="VFQ61_33065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9959384.1"
                     /translation="MLLDESRVYEQGAKDFRRTLTQIVRHHYEERRRRVLQALDREIK
                     SEKQGLDEARNEAIARLERFIERYSGTNSDPEATPDAMFRLAALYEERGREKQESDIA
                     VGLEPAIQLYRRIIREYPKYAEIAAVHYYLAHAFTDAARIEEGQQAFRALVCSNRYSV
                     KDDPKDASKIFLEPLPQDHEDKYWGDWYNRHPLPLDQAGQKNARVNAARPRAAQRLAG
                     GDFDEVTYKDPYTGCQPIAQNTLPGEEPRYLAEVWWQLGNYHFDQIDPRGGPFNLNRA
                     VSGYQQSMAYKKPPIYGVSMYKLAWSYFKQQRYRTAVDGFVKLLYYTDEQEAQTGDPG
                     ADFRAEAYTYIAGSLTYVDFDGPPADDPYIPRSDVLDTETDPVLAERKMSIAMQRVQD
                     PALIPQDRKWTVEIYKALAQEFIEITQNRNAVATLELTLQKFPMDRDAPVMQNKVAEL
                     YDQLARLSPENSSARAEYASKALEARTRLAQYVGTTPWVDANRDDPEALATAEQLVRG
                     GLKRAAADHTNFARSYYEKALELNNEGEQRALIEKSIAEYRLAETGWAAYIAQEPTAV
                     DAYESRFWLADARYWVVVLQVALGRSPNPEEIRAAREAAVTVRDSNEDDKYLQPSAYY
                     VVTIAEKHLDDEYRKFEQSNGREGLEKRMEVRFSGDGGDRKVVRDQVPPLVIEAVKAR
                     DEYNARIPLDRDPQHNGLLYAFQSADLYFVYGQFDEGRRRFTPLYEQNCGKNEWGYKS
                     WEKLISMSNFEGNAEESRRLAEGKSCAYDEDTKKAEEAIRKPVKQGVAYLEARRLYEE
                     AEKMPEGPARNKKWREAAAAYKVALDAAPDRDEAPEAAMNGAYAYKQVGEYDKAIEMY
                     ELFISRYGSAQTLNKLKNGDPKAQPPVAPDPKKYEDRVGYLKNAYDALASAYVLFFNY
                     PRAAETYDKIANNDNFKQADRREASRQALSLYSSLGDRTGMTRARQKFLDLGASPKEM
                     AEADFIVASAELKRWDPYSPDTGGNATARRAAQRAMDDYYDANKNKDPAAQFVVQAAY
                     WGARTRKAVTSGDTNKYWQNTINAFDKWKRLAPQEAGRSTALGSREAGMAAEGEFALV
                     DEDLTKKFDYESGFHRYKGTVVEVLQKYRNDVVEARRWDERLQHVIDAYLSPEWTAAA
                     LARQGTVWDSVRTGLYNTRPPELRMFDAKQEALLKRAESSDNPDLQARADEIRVSVQQ
                     GFRDAREKELAGADELMVDRYGKAIMVARRYSVSNPAVTRAIRRLAFMTEVVGEAKMR
                     QYTASVKDLNYTDGMFLRMRPGQVAAPKPEGWPPPLPVMVQ"
     gene            4094..5407
                     /locus_tag="VFQ61_33070"
     CDS             4094..5407
                     /locus_tag="VFQ61_33070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9959385.1"
                     /translation="MTRIIGVTALGLALAACGSSAPKPAAAPDTKVEAPPTPAETKTE
                     PSGQFAVSDAPTGGESAERPKMSAAATGPYMAGMNAFRNGDLAGAKTQFTQATQADTK
                     AYQAFYSLGVVKERLGDTAGALTAYRQSTQIVPDYEPAIVAYTTLLARMGRTDDALEY
                     INGRLSAMPKSAAVSDALAEVKSVQGDSGAAQRYAQEALKKNPDYRPAMVTIARDHYR
                     ARRLDLALYALKGVLDGYGAENPPRDKNNAEARLIRGLIYKEQNLRGPAIEEFRAALD
                     LRPDLVEARVQLATYLLEAGNAAEAATLLEGALRYQLDHVLAHLNLGDAYRLLGKVAE
                     SRREREWVSPTDPRLYQVHYTLGVLYLTADNVPGLTPMQAVDRAIDQLELYKKQRPRQ
                     AGVNDDTDELITRAKAKRGVLQAKEEEAKAAAQPAPAGENPAPAK"
     gene            5524..5778
                     /locus_tag="VFQ61_33075"
     CDS             5524..5778
                     /locus_tag="VFQ61_33075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959386.1"
                     /translation="MSKARTQILPLFAVALSALLSTGAAHAQKKGNRVVTISEVTIVG
                     RVQKPIAAVDVSRIQPKLTLAELRQPFLDRIEKAISRDPF"
     gene            5836..>6183
                     /locus_tag="VFQ61_33080"
     CDS             5836..>6183
                     /locus_tag="VFQ61_33080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959387.1"
                     /translation="MGLAVLAWAIALGCVFWVWRYTRRLQQKALPSAGELAEEARLLA
                     PSDASEHVFREVERLLSDARDEYRPRNELIIGLRRVALASGTGLSLLVLTRGVSLAQL
                     PAALVTFAAGVAGA"
BASE COUNT         1280 a         1910 c         2031 g          962 t
ORIGIN      
        1 cggcttcgac gagccccgcc tcaacggggg ctgcgcccgc ggtcgcgccc gccgttactc
       61 cgcgccgaga ggtgccaagg ccgaaggccc cgacgccgca gcagctgcgc gcgtaccaga
      121 tgttgctcga tgaatcgcgc gtgtacgagc agggcgccaa ggattttcgt cgcaccctga
      181 ctcagatcgt gcggcaccac tacgaagagc ggcgacggcg cgtgctgcag gctctggatc
      241 gggagatcaa atccgaaaag cagggcctcg acgaggctcg caacgaggcg atcgctcggc
      301 tcgagcgctt catcgagcgt tacagcggca cgaactccga cccagaggcg acgccggacg
      361 caatgtttcg cttggcggcg ctctacgaag aacgtggccg tgagaagcag gagtcggaca
      421 tcgccgtggg cctcgagccc gcgattcaac tctaccggcg catcatccgg gagtacccga
      481 agtacgcgga gatcgcggcg gttcactatt acctcgcgca cgccttcacg gacgccgcgc
      541 ggatcgagga gggacagcag gctttccgtg ctctcgtctg ctccaatcgt tacagcgtca
      601 aagacgatcc caaggacgca tcgaagatct tcctcgagcc cctacctcaa gaccacgagg
      661 acaagtattg gggtgattgg tacaaccgtc acccgctgcc gctcgatcaa gctgggcaaa
      721 agaacgcccg ggtgaacgcg gcgcggccgc gcgcggcgca gcgtttggcg ggcggcgact
      781 tcgacgaggt gacgtacaag gatccgtaca cgggttgcca gccgatcgcg cagaatactc
      841 tgccgggtga agaaccgcgc tacttggcgg aagtttggtg gcagctcggc aactaccact
      901 tcgatcaaat cgatccgcgt ggtggcccgt tcaacctgaa tcgtgcggtg agcggctatc
      961 agcagtcgat ggcctacaag aagccgccga tttacggcgt ctccatgtac aagctggctt
     1021 ggagctattt caagcaacag cgttaccgca ccgccgtgga tggcttcgtc aagctgctct
     1081 actacacgga cgaacaagaa gcgcagactg gtgatcctgg cgctgacttc cgggcggagg
     1141 cctacacgta catcgcgggc tcactcacgt acgttgactt cgacggtccg cccgcggacg
     1201 atccatacat cccgcgcagt gacgtgctgg acaccgaaac ggatccggtg ctcgccgaac
     1261 gcaagatgag catcgcgatg caacgcgtgc aggatccggc gctgatcccg caggatcgca
     1321 agtggacggt cgagatctac aaggcgctcg cgcaggagtt catcgagatc acgcagaacc
     1381 ggaacgcggt cgcgactctg gagctgaccc tgcaaaagtt cccgatggat cgcgacgcgc
     1441 ccgtcatgca gaacaaagtg gcggagctgt acgatcagct agcccggctc tcgccggaaa
     1501 actcgtcggc gcgcgccgag tatgcgagca aggcgctcga ggcgcgcacg cggcttgcac
     1561 aatacgtggg aaccacgccc tgggtggatg ccaatcgtga tgaccccgag gcgttggcca
     1621 cggccgagca gctcgtccgc ggtggcctga agcgagcggc ggcggaccat acgaactttg
     1681 cgcgctcgta ttacgagaag gcgctggagc tcaacaacga gggtgagcag cgcgcgctga
     1741 tcgagaagtc gatcgccgag tatcgcctgg ccgagacggg atgggcggcg tacatcgcgc
     1801 aggagcccac cgcggtggac gcgtacgaga gccgtttctg gctggcggac gcccgctact
     1861 gggtggtggt gttgcaggta gcgctcggtc gcagcccgaa cccggaggag atccgcgccg
     1921 cgcgcgaagc ggccgtcacg gtgcgagact cgaacgagga cgacaagtac ctgcagccgt
     1981 ccgcttatta cgtggtgacg atcgccgaga aacacctcga cgacgagtac cggaagttcg
     2041 aacagagcaa cggtcgcgaa gggctcgaga agcgcatgga agtgcgtttc agcggcgacg
     2101 gcggtgatcg caaggtggtt cgggaccaag tgccgccgct cgtgattgaa gccgtcaaag
     2161 cgcgagacga atacaacgcg cgcatcccgc tcgatcgcga tcctcaacac aatgggctgc
     2221 tgtacgcatt ccagtctgcg gacctgtact tcgtgtacgg ccagttcgac gaggggcgcc
     2281 ggcgctttac cccgctctac gagcaaaact gcggcaagaa cgagtggggc tacaagtcct
     2341 gggagaagct catcagcatg agcaacttcg agggcaacgc ggaggagagc cgtcgcctcg
     2401 ccgaaggtaa gagctgcgcg tacgacgagg acacgaagaa agcagaggag gcgatccgca
     2461 agcccgtcaa gcagggcgtg gcgtacctgg aggcccgccg gctctacgag gaagcggaga
     2521 aaatgccgga agggcccgcg cgcaacaaga agtggcgtga ggcggcggcg gcatacaagg
     2581 tggcgctgga cgcggcacct gaccgcgacg aggcgccgga agcggcgatg aacggcgctt
     2641 atgcctacaa gcaggtgggt gaatacgaca aagccatcga gatgtacgag ctgttcatct
     2701 cgcgctacgg cagcgcgcag acactgaaca aactcaagaa cggcgatccc aaagcacaac
     2761 cgccggtcgc gcccgacccg aagaagtacg aggatcgggt gggctacctg aagaacgcgt
     2821 acgacgcctt ggcttcggct tacgtactgt tcttcaacta tccgcgggca gcggagacgt
     2881 acgacaagat cgcgaacaac gacaacttca agcaagcgga tcggcgcgaa gcctctcgcc
     2941 aggccctctc gctgtactcg agcttgggtg atcgtacggg catgacacgc gcgcgccaaa
     3001 agttcctgga tttgggcgcg tcccccaaag aaatggccga agccgacttc atcgtcgcga
     3061 gtgcagagct caagcgctgg gatccgtaca gccccgatac gggtggtaac gcgaccgcgc
     3121 ggcgggcggc acagcgtgcg atggacgatt actacgacgc caacaagaac aaggacccgg
     3181 cggcgcagtt cgtggtccaa gcggcatact ggggcgcccg cacgcgcaag gccgtgactt
     3241 ccggagacac gaacaagtac tggcagaaca cgatcaacgc gttcgacaag tggaagcgcc
     3301 tcgcgcctca ggaggcgggc cggagcaccg ctctcggctc tcgtgaagcg ggcatggctg
     3361 cggaagggga gtttgcgctg gtcgacgaag atttgaccaa gaagttcgac tacgagtcgg
     3421 gctttcaccg ttacaagggc accgtcgtgg aggtgcttca gaagtaccgc aacgacgtgg
     3481 tggaggcgcg acgctgggac gagaggcttc agcacgtgat cgacgcttac ctctccccgg
     3541 agtggaccgc cgcggccctg gcccgccaag gtacggtctg ggactccgtc cgtacggggc
     3601 tatacaacac gcggcctccc gagttgcgga tgttcgacgc caagcaggag gccctgctga
     3661 aacgcgcaga aagcagcgac aatccggacc tccaggcgcg tgccgacgag atccgggtca
     3721 gcgtgcagca agggttccga gacgcacggg agaaggagct cgcgggcgcc gacgagttga
     3781 tggtcgatcg ctacggcaaa gccatcatgg tggctcgccg ttacagtgtc tcgaatcctg
     3841 cggtgacccg cgcgattcgc cgcttggcgt tcatgaccga ggtcgtgggc gaagccaaga
     3901 tgcgccagta caccgcgtcc gtgaaggact tgaactacac ggacggcatg ttcttgcgca
     3961 tgcgcccagg tcaggttgca gctcctaaac ccgagggttg gccgccgcca ctgccggtga
     4021 tggtgcaatg agcgcgttcg agagcacggg ttcaatgaac atggttgctg atctctctgg
     4081 aagggtattc ggagtgaccc gaatcatcgg cgtcacggcc ttgggcctgg cgctcgcggc
     4141 ctgcggctcg agcgcgccca agcccgccgc tgcacccgat acaaaggtcg aagcaccgcc
     4201 cacaccggcc gaaacgaaga ccgagccgag cggacagttc gcggtctcgg acgcgccgac
     4261 gggcggtgaa tctgccgagc gacccaagat gagcgcggcc gcgacggggc cgtacatggc
     4321 gggtatgaat gcgtttcgca acggtgacct cgccggggcc aaaacgcagt tcacgcaggc
     4381 gacgcaggcg gacacgaagg cctatcaggc attctattcc ctgggcgtag tcaaggaacg
     4441 cttgggggac acggcggggg cgctcacggc gtatcgccag tcgacccaga tcgtgcccga
     4501 ctacgagcca gcgatcgtgg cctacaccac gctgctggct cgcatgggcc gcaccgacga
     4561 cgcgctcgag tacatcaatg gtcggctctc cgctatgccc aagagcgcgg ccgtgagcga
     4621 cgcgctggcc gaagtaaagt cggtgcaagg cgattcgggc gccgcccagc gctacgctca
     4681 agaagcgctc aagaagaacc cggattatcg cccggccatg gtcacgatcg cccgcgacca
     4741 ctatcgcgct cgcaggctcg atctagcgct ctatgcactg aagggcgtgc tcgacggata
     4801 cggagccgag aacccgccgc gcgacaagaa caacgccgag gcgcgcctga tccgcggcct
     4861 gatttacaag gaacagaacc tgcgcggtcc cgccatcgaa gagttcagag cggccctgga
     4921 tttgaggccc gatctcgtcg aagcgcgtgt gcagctcgcc acttacctgc tcgaggcggg
     4981 taacgcggca gaagccgcga cgctgctcga gggggcgttg cgttaccagc tcgatcacgt
     5041 gctggcgcat ctaaatctcg gcgacgccta ccgattgctc ggcaaggttg ccgagtcacg
     5101 gcgtgagcgc gagtgggtgt caccgacgga tccgcgcctc taccaggtcc attacaccct
     5161 gggcgtgctg tacctgacgg cggacaacgt tccagggctc acgccaatgc aagccgtgga
     5221 tcgggctatc gatcaactgg agctctacaa gaaacagcgt ccgcgccagg cgggcgtgaa
     5281 cgacgacacc gatgagctca tcacgcgtgc caaagcgaaa cgtggcgtgc tccaagccaa
     5341 ggaagaagag gcgaaggctg ccgcacagcc agcgcccgcg ggtgagaatc ccgctcctgc
     5401 caagtgaccg cctccgtctt ctacagtcag gtttcgaccg agccagccgc cccaacaaaa
     5461 cgtgctgccc cgaaaacgtg ctgccccgaa aacgtgctgc cctaaaaaac aaggtctaac
     5521 cgcatgtcga aagctcgcac tcagattctg ccgttgtttg ccgttgcgct gagcgccctg
     5581 ctgtcgacgg gggccgcgca cgcgcaaaag aagggtaacc gcgtcgtgac catcagcgag
     5641 gtaaccatcg tcggtcgcgt acagaagccg atcgcagcgg tggacgtcag ccgcattcaa
     5701 cccaagctca cgctggcgga gttgcggcag cccttcttgg atcgcatcga aaaagccatt
     5761 tcgcgcgatc cgttttgagc aacatcgatg tgggaacgga tggccccgac cgagcgcggt
     5821 aaaacaggct agcgcatggg gctcgctgtt ttggcgtggg ctatcgcgct cggctgtgtg
     5881 ttttgggtct ggcgttacac ccgaaggctc cagcagaaag ctttgccctc tgccggtgaa
     5941 ctggcggaag aggcgcgttt actggcgccg agcgacgcga gtgagcacgt ctttcgagaa
     6001 gtggagcgct tgctctccga cgcccgtgac gagtatcgcc cgcgcaacga gctgatcatc
     6061 gggctgcgcc gcgtcgctct ggcgagcgga actgggctct cgttgctggt cctcacgcgc
     6121 ggggtgagcc tcgctcagct gccggccgcg ctcgtaacct tcgcggccgg ggtcgccggc
     6181 gca
//
LOCUS       DASTKU010000936         6532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_627358, whole genome shotgun sequence.
ACCESSION   DASTKU010000936 DASTKU010000000
VERSION     DASTKU010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627358"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..874
                     /gene="accB"
                     /locus_tag="VFQ61_33085"
     CDS             416..874
                     /gene="accB"
                     /locus_tag="VFQ61_33085"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET9959388.1"
                     /translation="MQIGLEQLEALLKTLDDKKVAEFEYEDEKIRLRVALGRVAQISA
                     VAPVVTQAPTAAPAASASAAPEAAAADPSIGFITSPFVGTFYRAPSPEADNFTEVGAT
                     VKKGQALCIIEAMKLMNEIEAEFGGTVVEILVENGKSVEFGQKLFKIKKS"
     gene            881..2245
                     /gene="accC"
                     /locus_tag="VFQ61_33090"
     CDS             881..2245
                     /gene="accC"
                     /locus_tag="VFQ61_33090"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET9959389.1"
                     /translation="MFRKVLIANRGEIAIRIIRACRELGIRTVAVHSEADAGALHVRF
                     ADEAVCIGPAAAAKSYLHIPALISAAEITAADAIHPGYGFLSENSEFAQVVERCGLHF
                     IGPRADAMQAWGDKLRARAMAKKFGLPLLPGSEALENATHAESEAKRVGFPVMIKARG
                     GGGGRGMRMVRNVSEVRRAFESATAEASTAFRNPELYLERFVERPKHIEFQALADQHG
                     QVWTLGERECSLQRKNQKLVEEAPSPSMNETLRAEMGERIRHAIRESGYTSLGTLEFL
                     MDETGELYFMEMNTRVQVEHPVTEMSTGVDLVVEQIKAAAGERLDLPDTRSWKFRGHS
                     IECRIMAEDPVTFAPWPGRIQEYHPPGGAGVRVDSGVFGGWTVPAHYDSLLAKVIVHG
                     PTREIAVRRMRRALDEFIISGIRTNIPLQKRLLDLPEVRDGSMTTRTVENMMARDAEQ
                     RARN"
     gene            complement(2317..2682)
                     /locus_tag="VFQ61_33095"
     CDS             complement(2317..2682)
                     /locus_tag="VFQ61_33095"
                     /inference="COORDINATES: protein motif:HMM:NF021165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959390.1"
                     /translation="MRGTSCSWADASRIARLASVIVLAAGLTGCASSLPPPTAAQVQA
                     AQEREPAASLADLTRGRDLYVQKCGNCHALRDPAGMNREQWDHAIHEMRTEQNVKLTE
                     AEHQDILRYLDAASRAPAR"
     gene            complement(2679..3653)
                     /gene="hflC"
                     /locus_tag="VFQ61_33100"
     CDS             complement(2679..3653)
                     /gene="hflC"
                     /locus_tag="VFQ61_33100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HET9959391.1"
                     /translation="MSLNLRLLALLLVALVLLGSSAYTVQETEQVIVTQFGKPNPTPI
                     SEAGLHFKIPFIQEANSIEKRILEWDGPRSEMPTRDKLYISVDSFARWRVVRPLEYFR
                     RLRDERSAQSRLDDILGSETRNAIAKHDLIEVIRTTKERVPLLDQTMQAGPDGAGSVG
                     VLHPIRKGRKSIEQEVHAAAKDKLAEFGIELLDVRFKRINYNPSVVDKIYERMISERQ
                     QIASRFRSEGEGEAAKILGDKERDLSVIESEAYKKVEEVRGAADAKATAIYAAAYSPT
                     PEARALYEFVKTMETYRKVVNPEDTLVLSTQSDLFQFLERAAGGGAKK"
     gene            complement(3650..4618)
                     /gene="hflK"
                     /locus_tag="VFQ61_33105"
     CDS             complement(3650..4618)
                     /gene="hflK"
                     /locus_tag="VFQ61_33105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HET9959392.1"
                     /translation="MALPRSLPLNWLRTGLGILGLLVLGWTVFYTVPAESEAVVLRLG
                     QFHTVVPSGLHFKLPLGVDSVLIVPVKRQLKQEFGFGTQGNSNPWQAAPETEWGEEKT
                     MVTGDLNVALVEWVVQYRIERPDHYLFRVRNPDATLRDASENVMREVIGDRTVDEVIT
                     IGRQEIEVEALQKLQALVTSYDMGVKIDQVQLKNVNPPEKVQASFNEVNQAQQEKERA
                     VNVANGEYNKEVPRARGRAAQAIRNAEGYAQKRINEAEGDVARFKALLDEYVKAPEVT
                     RKRLYLETMAELLPAVKSKVIVSQDVKGVLPLLNLKTATELKGAAQ"
     gene            complement(4668..5219)
                     /locus_tag="VFQ61_33110"
     CDS             complement(4668..5219)
                     /locus_tag="VFQ61_33110"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9959393.1"
                     /translation="MDEKNPYQVRGCRQIYDNPWITVREHAITTPSGREGIYGVVEFK
                     NQAVGVVPYEAGRIWLVGQYRFPLSAYSWEIPEGGAPHDEAPEACAHRELLEETGLRA
                     RRLDKLFSMHLSNSVSNEIAHVFLARELEHGEAQPEDTEQLVVRPVTLSEALALVLSG
                     EITDSMSVAAVFRLELMRREGQL"
     gene            complement(5250..>6532)
                     /locus_tag="VFQ61_33115"
     CDS             complement(5250..>6532)
                     /locus_tag="VFQ61_33115"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HET9959394.1"
                     /translation="DLMKMLLSVQKRNGSAMHQFNPLTMHANAGDSRERLDRPNYYGD
                     DHLWIVIAVCWYLKETGDLAFLEEVIPFYEKDQNELPLETGTVREHLMRSVAFTKGDL
                     GQHGLPLLGFADWNDTVNLPTGAESMFNANLYGVALRELIELCEHLGDRGTAAQLEAD
                     FAKMKETFNACAWDGEWFVRYFDADGSPVGSRTNSEGQIYTNGQSWPVLSGFASPERA
                     RTALDSVYRLLNTKNGIKLSWPGYKGWDPAKGGVTTYPPGAKENGGIFLHANPWVMIA
                     ETRLGNGDRAYEYYHQINPAAKNDRIDEFECEPYAYPQNILGDEHPQFGLARNSWLSG
                     TSAWTYTAATKYILGIRTVHGGLAIDPCIPKAWEGFEVTRVFRGATYEISVKNPKRVS
                     RGVAELKVNGELVPGNVVRVASPGSRVKVEATLG"
BASE COUNT         1132 a         2041 c         2049 g         1310 t
ORIGIN      
        1 attcgggacg acgacgcgcg catcacccgc cggcagcccg cgcggactct cgaaaatcca
       61 gaagtgatgg atgaacggcg cgatttccgg atgcggcgcg actgtcctaa gcaacatgcg
      121 ttctttgcca gcgggacctc aacgccgccg ccggaggatt ttgcaggata gcttggcgac
      181 gcagaggggg aagtcgcgtg cggcatactg cgtcgcgctt tacgctgtgt cggtcgatac
      241 gctgaccgcg cagccgaatc cggaggttct accagcacgt tgccgcacgg cgtcggaggg
      301 ggctccacag gaacggcgtc gtttgtgcac gcggtgggtt gtatccagac gattgcccac
      361 gctaacgtcc gcgggcccgc tcaggcagcc tccgagcggt gaggtgacat agctcatgca
      421 aattggtctg gaacagctcg aagccctgct gaagacgctc gatgacaaga aggtcgccga
      481 gttcgaatac gaagacgaga agatccgctt gagagtggcg ctcggtcgcg tcgctcaaat
      541 ctctgccgtg gctcccgtcg tcacgcaggc tcccaccgcc gcaccggccg caagtgccag
      601 cgccgccccc gaagccgcgg cggcggatcc gagcatagga ttcatcactt cgcccttcgt
      661 gggcacgttc tatcgtgcgc cctcgccgga ggctgacaac ttcaccgagg ttggcgcgac
      721 cgtgaagaag gggcaggcgc tctgtattat cgaagcgatg aagctgatga acgaaatcga
      781 ggcagaattc ggcggcacgg tcgtggagat cctcgtcgaa aacggcaaga gcgtcgagtt
      841 cgggcagaag ctcttcaaaa tcaagaagtc ctgagcaccg atgtttcgca aggtcttgat
      901 cgccaatcgt ggcgagatcg cgattcgtat cattcgcgcc tgccgtgagc tcggcatccg
      961 cacggtggcg gtccattccg aggccgacgc gggcgcgctt cacgttcgct tcgcggacga
     1021 ggccgtctgc attggacccg cggcggccgc caagagctac ctgcacattc cggcgttgat
     1081 cagcgccgcc gaaattacgg cggcggacgc cattcacccg ggctatgggt tcttgtcgga
     1141 gaactccgag tttgctcagg tggtggagcg ctgcgggttg cacttcattg ggccgcgcgc
     1201 cgatgcgatg caagcctggg gcgacaagct gcgcgctcgc gccatggcca agaaattcgg
     1261 gcttccgctg ctgcccggca gtgaggcact ggagaacgcc actcacgccg agtccgaggc
     1321 caagcgggtc ggttttcccg tgatgatcaa ggcgcgcgga ggtggcggag ggcgcggtat
     1381 gcgcatggtc cgcaacgtct ccgaagtgcg tcgtgctttc gagtccgcca ccgcggaggc
     1441 ttcgacggcg ttccggaacc ccgagctgta tttggagcgc ttcgtcgagc gtcccaagca
     1501 catcgaattc caagccctcg ccgatcagca tggtcaggtg tggacgctcg gcgagcgcga
     1561 gtgctcgctg caacgcaaga accagaagct cgtcgaagag gctccgagcc cttcgatgaa
     1621 cgagacgttg cgcgccgaga tgggcgagcg cattcgtcac gcaatccggg agagcggtta
     1681 cacctcgctc ggtacgctcg agttcttgat ggacgagacg ggtgagctct acttcatgga
     1741 gatgaacacc cgcgttcagg tcgagcaccc cgtcaccgag atgtcgaccg gggtggactt
     1801 ggtggtcgag cagatcaagg ccgccgccgg ggagcgtctg gatcttccgg atacgcgatc
     1861 gtggaagttc cgcggccact ccatcgagtg ccgcatcatg gcggaggacc cggtgacgtt
     1921 cgcgccgtgg ccaggtcgca tccaagagta tcatccgccc gggggagccg gtgtccgcgt
     1981 ggattcgggc gtattcggcg gctggacggt gcccgcccac tacgactcgt tgctagcgaa
     2041 ggtgatcgtt cacgggccga cccgcgagat cgcggtgcgt cggatgcgac gcgcactcga
     2101 cgagttcatc atctcgggta ttcgcacgaa tatcccgctg cagaagcgct tgctcgactt
     2161 gcccgaggtg cgcgacggca gcatgactac gcgcacggtc gagaacatga tggctcggga
     2221 cgccgagcaa cgcgcgcgga actgagagct tggttccaat acgaagtcga cgtcggtaac
     2281 gccgacgtcg acttctgtaa ttcgctcgct ggtctgtcag cgcgctggcg cgcgactggc
     2341 ggcgtcgaga taacgcagga tgtcttggtg ctcggcttcg gtgagcttca cgttctgctc
     2401 ggtgcgcatc tcgtgaatgg cgtggtccca ctgctcgcgg ttcatgccgg cgggatcgcg
     2461 gagagcgtga cagttgccgc acttctgcac atacaaatcg cggcctcgag tgagatccgc
     2521 taggctggcg gcgggctcac gctcctgcgc cgcttggacc tgcgccgccg tcggaggggg
     2581 caagctgctc gcgcagcctg tcaagccagc cgcgagcacg ataacgctcg ccagccgcgc
     2641 gatgcgagaa gcgtccgccc acgagcacga ggtgcccctc atttcttggc tcccccgccc
     2701 gcggcgcgct ccaggaactg gaatagatcg ctctgggtcg acaaaacgag agtgtcctcg
     2761 gggttaacga ccttacggta agtctccatc gtcttcacga attcgtagag ggctcgcgct
     2821 tcgggggtag gtgagtaagc ggcagcataa atcgcggtcg ctttggcgtc cgcggcgccc
     2881 cggacttctt cgaccttctt gtaggcctcg gactcgatga cgctcaagtc gcgctcttta
     2941 tcgcccaaaa tcttggcggc ttcgccttca ccctcggagc gaaagcgcga ggcaatctgt
     3001 tgccgctccg agatcatgcg ttcgtaaatc ttgtctacga ccgaggggtt atagttaatc
     3061 cgcttgaagc gaacgtcgag tagctcgata ccaaattcgg cgagcttgtc tttggcggcc
     3121 gcgtgcacct cttgctcgat gctcttgcgc cctttgcgga tgggatggag cactccgacg
     3181 ctgcccgcac cgtccggccc ggcctgcatg gtttgatcga gcagcggcac gcgctctttg
     3241 gtggtgcgga tcacctcgat cagatcgtgt ttggcgatgg cgttgcgggt ctcgctgccc
     3301 aagatgtcat cgagccggga ctgcgcgctc cgctcgtccc gcagccggcg aaagtactcg
     3361 agcggccgca cgacgcgcca gcgcgcgaac gaatcgaccg agatgtacag cttgtcccgg
     3421 gtgggcatct cgctgcgtgg cccatcccac tcgagaatgc gcttctcgat cgagttcgcc
     3481 tcttgaatga acgggatttt gaaatgcagg cccgcctccg aaatcggggt cgggttgggc
     3541 ttgccgaact gagtcacgat cacctgctcg gtctcttgca ccgtgtaggc gctcgacccg
     3601 agcaacacca gggccaccag cagcagcgcg agcaggcgta ggttcaagct cattgggccg
     3661 ctcctttgag ctccgtggcc gtcttcaggt tcaagagcgg caacacacct ttcacgtctt
     3721 ggctcacgat taccttactt ttcacagcgg gcagcagttc ggccatggtc tcgaggtaca
     3781 ggcgtttgcg cgtgacttcg ggcgccttga cgtactcgtc tagcagggct ttgaagcgtg
     3841 ccacatcacc ttcggcctcg ttgatgcgtt tctgcgcgta gccctcggcg ttacggatgg
     3901 cctgcgcggc tcggcctcgc gcgcgcggca cttccttgtt gtactcgccg ttggcgacgt
     3961 tcacggcacg ttctttctct tgttgggctt ggttcacctc gttgaacgag gcctgcacct
     4021 tttccggcgg gttcacgttc ttgagctgca cctggtcgat cttgaccccc atgtcgtagc
     4081 tcgtgaccaa cgcctgcagt ttctgcaggg cctcgacttc gatctcctgg cggccgatcg
     4141 tgatcacttc gtcgaccgtg cgatcaccga tcacctcacg catcacgttc tccgacgcgt
     4201 cccgcaacgt tgcgtccggg ttgcgcaccc ggaacaagta gtggtccggg cgctcgatcc
     4261 ggtattgcac cacccactcc acgagcgcga cgttgaggtc gcccgtgacc atcgtcttct
     4321 cttcgcccca ttcggtttcg ggggcggcct gccaaggatt gctattgccc tgcgtgccga
     4381 acccgaactc ctgcttgagt tggcgtttga ctggcacgat caacacgcta tccacgccga
     4441 gcggcagctt gaagtgcagc ccggagggga ccacggtgtg gaactggccg agccgcagca
     4501 cgaccgcttc cgactcggcc ggcaccgtgt agaacacggt ccatccgagc actaacaagc
     4561 ctagaatacc caagccggtg cgcagccagt tcaacggcaa agagcgcggc aaagccatgg
     4621 ggcgcagcat agcattattg ctgcggcgcc cgcgctcgag agctggatta tagctgccct
     4681 tcgcgccgca tgagctcgag tctaaaaacg gcggccacgc tcatcgagtc cgtaatctcg
     4741 ccgctcagga ccagcgcgag cgcttcactc agtgtaaccg gacggaccac tagttgttcc
     4801 gtgtcttcgg gctgcgcttc tccgtgttcg agctcacgcg ccaagaacac gtgcgcgatc
     4861 tcgttggaca cggagttcga caggtgcatc gagaagagct tatcgagccg gcgggcgcgg
     4921 aggccggtct cctcgagcag ctcacggtgc gcacaggcct cgggcgcttc gtcgtgcgga
     4981 gctccgcctt ccggaatttc ccaagagtag gcggaaagcg gaaatcgata ttgcccgact
     5041 agccagattc gtcccgcctc gtaaggcacc acgcctacgg cctgattctt gaattcgacg
     5101 acgccataga tcccctcgcg cccgctcggg gtggtgatgg cgtgctctcg gacggtgatc
     5161 cacgggttgt cgtaaatctg ccggcaaccc cttacctgat atggattctt ctcgtccaag
     5221 agcgcgcctt ccttgggtat cagctcagct cagccgagcg ttgcctctac tttcacgcgg
     5281 ctgcccggtg aagctactcg caccacgttc ccggggacga gctctccatt cacctttagt
     5341 tcggccaccc cgcgcgacac gcgtttcggg ttcttcacgc tgatttcata ggttgccccg
     5401 cggaacacgc gggtaacctc gaagccctcc catgccttgg ggatgcatgg atcgatggcg
     5461 agcccgccgt gcacggtacg gattccgaga atgtatttgg tggcggctgt gtacgtccac
     5521 gccgacgtgc cgctcagcca gctgttgcgg gccaacccga actgcggatg ttcgtcgccc
     5581 aagatattct gcggatacgc gtacggctcg cactcgaact cgtcgatgcg gtcgttcttc
     5641 gccgccgggt tgatctggtg atagtactcg taagcgcgat cgccgttgcc caggcgcgtc
     5701 tccgcgatca ttacccaggg attggcgtgc aggaagatcc cgccattttc cttggcgccc
     5761 ggggggtaag tcgtcacgcc gcccttcgcg ggatcccacc ctttatagcc gggccagctg
     5821 agcttgatgc cgttcttggt gttgagcagc cgatatacgc tatcgagtgc ggtgcgcgcc
     5881 cgctcggggc tcgcgaagcc gctgagcacc ggccaagatt gaccgttggt atagatctgc
     5941 ccctcgctgt tcgtacggct cccgacaggg ctgccgtcgg cgtcgaagta acggacgaac
     6001 cactcgccgt cccacgcgca ggcgttgaac gtctccttca tcttggcgaa atccgcctcg
     6061 agctgcgcgg cggttccccg gtcaccgagg tgctcgcaga gctcgatcaa ctcacgcagc
     6121 gcgacgccat acaggttggc gttgaacatg gattcggcac cggtgggtag attgacggta
     6181 tcgttccagt cggcgaatcc gagcagtggc agcccgtgct ggccgaggtc gcccttggtg
     6241 aaggccacgc tgcgcatcaa gtgttcgcgc accgtgccgg tctcgagggg aagctcgttt
     6301 tggtctttct cgtagaaggg aatgacctct tccaagaacg cgagatcgcc agtctctttc
     6361 aggtaccaac agaccgcgat cacgatccat aggtgatcat cgccgtagta attggggcgg
     6421 tccagccgct cgcgcgagtc acccgcgttg gcgtgcatgg tgagcgggtt gaattggtgc
     6481 atggccgagc cattgcgctt ctgtacgctg agcagcatct tcatcaaatc gc
//
LOCUS       DASTKU010000937         4497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_404409, whole genome shotgun sequence.
ACCESSION   DASTKU010000937 DASTKU010000000
VERSION     DASTKU010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404409"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..331
                     /locus_tag="VFQ61_33120"
     CDS             95..331
                     /locus_tag="VFQ61_33120"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET9959395.1"
                     /translation="MLICHCNLVTDREIREAIRAGAHTVCAVSEACDAGSCCGGCRPM
                     IEKLIRLERRALSQPPASTYAREALMDEAGKSEG"
     gene            328..1776
                     /locus_tag="VFQ61_33125"
     CDS             328..1776
                     /locus_tag="VFQ61_33125"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959396.1"
                     /translation="MTLRSRPAARRLGVATWLVFSLSTARGYGAPSISGAHGIAFSPS
                     QAGALLVRTPYGVFHSRDGGQTADFLCDEVMTAARDAPAEVVFVNGGALLASRPPSWL
                     TLGLPAVLPGTPTENVPHLARSEDGCNWQTFGTEGELSQFAVDPSHPERVLAVGFGIE
                     ELEPSDSGEPRERSRARLMLSTDAGESFARLLELPPETTPLSVVIAPSNSERVYVAAW
                     AQRSEGTGSRLLVSSDGGSEFREFALPGGERLAARVVAVHPLRENRVYLQLQDSGETQ
                     LLVSDDAGETFRSFFTGAENLTGFAIADDGRELYFGSRSGTWAARGDGSEPERRSELA
                     VTCLAYGDFALFACPSDSSPYALARSTDGGRSFTHSLRFADTCGSSGCSEGTDVQLTC
                     PAVLPEIASTLEVKACAPSSGWSDGTAGAGYEPAQVSRVRARGGCQFGYSAAAASGWA
                     GWFSAIVLWSVRIGARNRRHRPQSRTRSPLAE"
     gene            1802..2545
                     /locus_tag="VFQ61_33130"
     CDS             1802..2545
                     /locus_tag="VFQ61_33130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959397.1"
                     /translation="MSAARLSNAGVTTLGVENGLSARKSGRITLRRGLVHSLLLAAVT
                     GTLAAGCRSEDEPGTGSPSGPSIEDVLIEGTATDEALQALLDAKPIDQPVRYVAFDEP
                     AANAKLTATAPLTFTLYEPASAAHSLPPADFSHSPRKPAGRRQTDHSTWFARVARILN
                     PISIAHAHGTPFSGTGYLFDFQDARGKSSYAVFTSERSTILGLDVLADLKQVAQPLTL
                     HVTSADFEDNRIPSGGGPFAAGSLTFSIE"
     gene            complement(2550..2882)
                     /locus_tag="VFQ61_33135"
     CDS             complement(2550..2882)
                     /locus_tag="VFQ61_33135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959398.1"
                     /translation="MFYLFLLVLAVLSAGVYLVVFFQSVPGAAEERLGLLEPLPEDLG
                     VWKPDASTEEGKSAERAGLRREQRHYFDEAKGKLFLQVRYRSLENDEIVRADPDRVIK
                     RRRIRKPG"
     gene            3159..4469
                     /locus_tag="VFQ61_33140"
     CDS             3159..4469
                     /locus_tag="VFQ61_33140"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9959399.1"
                     /translation="MWSNSCGVIRTEETGKQGMALHKNVVVIGGGFGGMAVTRALAHE
                     KVQITLLDRRNHHLFQPLLYQVAMAGLSPAEIAVPIRSVLRKQKNVRVLLAEVTNVDV
                     TNRSVTLKEGPPLPYDYLVVATGSRTNYFGHDWDKVAPGLKDLEDALEIRRRVLLAFE
                     AAERETDPVQQRRLLTFVVIGGGPTGVELAGAIAELSDFVLARDFRSIHPESTRVILV
                     EGGARVLESFSPELSEKATESLREMGVELRTQMRVTGIDERGIVMGTERIEASTVLWA
                     AGVAANPLVQSLGAPTDRAGRVIVQNDCTVPGHPEVFVIGDAALFKPEGAERQLPGVS
                     PVAIQQGRFVAKEIARRIEGHTARQFVYRDKGSMATIGRSRAVAEMGGLKLSGFVAWL
                     FWLLVHIYYLIDFKNRLIVLINWAWAYVAYNRGARLITGREEKP"
BASE COUNT          807 a         1399 c         1461 g          830 t
ORIGIN      
        1 acgtaggtcc gttcggcttg ttgcctctga tattgacaat cattttcaat ccgctagctt
       61 cagccaagtc gcttttcgac cgtaggtact gagcgtgctg atctgtcact gcaacttggt
      121 tacggatcgt gagatccgag aagcaatccg tgccggagct cataccgtct gcgcagtgag
      181 tgaggcgtgt gacgccggca gctgctgcgg gggatgccgg ccaatgatcg aaaagctgat
      241 ccggctggag cgccgtgcgc tgagccagcc ccctgcctcg acctacgcgc gagaggcatt
      301 gatggacgaa gctggtaagt ccgagggatg acacttcgct cacgccccgc ggcgcgccgg
      361 ctcggcgttg ctacgtggct cgtgttttcg ctctcaaccg cgcgtgggta cggggcccca
      421 agcatcagcg gggcgcacgg gatcgccttt tcaccaagcc aagccggagc gctgctggtt
      481 cgcactccat atggcgtctt ccattcacga gatggcggcc agacggcgga ctttctctgt
      541 gatgaagtga tgacggccgc tcgcgatgca cccgcggagg tggtcttcgt gaatggggga
      601 gcactcctgg cgagcagacc gccgagttgg ctcacgctcg gattacccgc agtgctgccg
      661 ggcacaccca ccgaaaacgt gcctcacctg gcccgcagcg aagacggttg caactggcag
      721 acgttcggaa ccgaaggcga gctcagtcag ttcgcggtgg acccgagcca ccccgagcgc
      781 gtgctcgcgg ttggattcgg tatcgaagag ctcgaaccct cggactcggg tgagccccgc
      841 gaacgttccc gagcgaggct catgctttcg accgacgcag gcgagagctt cgcgcgattg
      901 ctcgaactgc cgccggaaac cactccgttg agcgtagtca tcgcgccctc caactcggag
      961 cgtgtctacg tcgcggcgtg ggcacagcga tcggagggta cgggctcacg attgttggtg
     1021 tcgtcggacg gagggagcga gttcagggag ttcgcgctcc cgggagggga aaggctcgcc
     1081 gcccgcgtgg tcgcggttca cccacttcgc gagaatcgcg tctacttgca gctccaggat
     1141 tcgggcgaga cacagctgct ggtgagcgat gatgctggcg agacctttcg aagtttcttc
     1201 acgggcgcgg agaaccttac cggcttcgcc atcgcagatg acgggcggga gctttacttt
     1261 ggaagccgct ccggaacttg ggccgcccga ggggacggca gcgaaccgga acgccgctcg
     1321 gagctagccg tgacttgcct ggcttacggc gacttcgcgc tgttcgcctg tccgagcgac
     1381 agcagcccgt atgccttggc gcgctccacg gatggtggac ggagcttcac tcattcgctg
     1441 agattcgcgg atacttgtgg gagcagcggc tgcagcgaag gcaccgatgt gcagctcact
     1501 tgtccggcgg ttctgccaga aatcgcgagc accctggagg tcaaggcgtg cgctccttct
     1561 tcgggctgga gtgacggcac ggccggagcc ggatacgagc ccgcccaagt gagccgggtg
     1621 cgagcgcgcg gcggctgcca attcggctac agcgctgcgg ctgcctccgg atgggcaggg
     1681 tggttttctg ccatcgtatt gtggagcgtg cgaattggcg ctcgtaaccg acggcatcgg
     1741 ccccagtcac gcacgcgttc tccgctggcc gaatgacgct tttgcggcaa actgccgatc
     1801 gatgtcggca gcacgcttga gcaacgcggg tgtgaccacg ctcggtgtcg agaacgggtt
     1861 atcagccagg aagtctgggc gaatcacgct ccggcgcggg ctcgtccact cgctgctcct
     1921 cgccgcggta acgggcactt tagcggcagg ttgtcggagc gaagatgagc ccggcaccgg
     1981 ctccccgagt gggcccagca tcgaagacgt gctgattgag gggacggcca cggatgaagc
     2041 tctgcaggcg ctactcgacg cgaaacccat agatcagccc gtgcgttacg ttgcattcga
     2101 cgagcccgcc gcaaacgcaa agcttacggc caccgcgccg ctcaccttca cgctatatga
     2161 gcccgcgagt gcggcgcact ccctgccgcc tgcagacttt tcgcacagcc cgcgcaaacc
     2221 ggcgggcaga aggcagacgg accactccac ttggttcgcg cgcgtcgcac ggatactgaa
     2281 tccaatcagc atcgcccacg cgcacggcac accgttcagc ggcactggct acttgttcga
     2341 ctttcaggat gcgcgaggaa agtcgagcta tgccgtgttc acttccgaga ggagcaccat
     2401 attggggttg gacgtgctcg cggatttgaa gcaggtggcg cagccactca cgctccacgt
     2461 cacctccgca gacttcgagg acaaccgcat tcctagcggc ggaggcccct tcgcagccgg
     2521 ctcgctgacg ttctcgatcg agtgagggct cacccgggct tgcgaattcg gcgacgcttg
     2581 atcacgcgat ccggatccgc gcgcacaatc tcgtcattct cgaggctacg gtaacggacc
     2641 tgcaagaaca gcttaccctt ggcttcgtcg aagtagtgcc gttgttccct gcgcagtccc
     2701 gcacgttcgg cggatttgcc ctcttcggtg ctcgcgtccg gtttccagac ccccaagtct
     2761 tccggcaacg gctcgagcag accgagccgc tcttccgccg cgccgggcac actttgaaag
     2821 aagaccacga ggtacacccc cgcgctcagc acggcgagca cgagcaagaa tagataaaac
     2881 acgtgtctcc tctcgttggt tacccagcct cgcccacgtg cgcaagcttt tccctgaaga
     2941 gcgctccgcg tgagcgccag aggcggtctt gaaccggtct gccccgaatc agcgggctct
     3001 ctggtgagcg cgtccgcgcg ccgggcatac gtccctactc tcggaacctc acgctcgcgg
     3061 attgaacgag cggtcggctc gtcgtgaaaa cgccccccgt cgagctgcct gagcaacgcc
     3121 tggagcggca tacggaatgc tagcgttccg agtaactcgt gtggagcaac tcgtgcggag
     3181 tgattcgcac ggaggagaca gggaagcagg gcatggcctt acacaagaac gtagtcgtga
     3241 tcggcggggg atttggcggc atggccgtga cacgcgcgct ggcccacgaa aaggtgcaga
     3301 tcacgctgct cgatcgccgc aatcaccatc tgtttcagcc gctgctctat caggtcgcca
     3361 tggcgggcct atcacccgcc gaaatcgcgg ttcccatccg cagcgtgctg cgcaagcaga
     3421 agaatgtgcg cgtgctcttg gctgaagtca ccaacgtgga cgtgaccaac cgaagcgtca
     3481 ccttgaaaga aggtccgccc ctaccctacg actatctggt agtcgcgacc gggtcgcgca
     3541 ccaactactt cggacatgat tgggacaagg tcgcgccagg tctcaaggat ttggaggacg
     3601 cgctcgagat ccggcggcgt gtcctgctcg cgttcgaagc cgcggagcgc gaaaccgatc
     3661 ccgttcagca acgccggctg ctcacgttcg tggtgattgg aggtggtccc accggtgtag
     3721 agctcgcggg agcaattgcg gagctgtccg acttcgtgct ggctcgcgac ttccgcagca
     3781 ttcaccctga atccacgcgc gtgattttgg tcgaaggggg cgcgcgcgta ctcgagagtt
     3841 tttcgcctga gctctccgag aaggcgaccg agagtctgcg cgagatggga gtggagctgc
     3901 gcacgcaaat gcgggttacc ggcatcgatg agcgcggcat cgtgatgggg acggaacgga
     3961 tcgaggcgtc cacggtgctg tgggccgcgg gtgtggccgc caacccgctc gttcaatcgc
     4021 tgggggcgcc gaccgaccgc gccggcaggg tcatcgttca gaacgactgc accgtgcccg
     4081 ggcaccccga ggtgttcgtg attggagacg ccgcgctgtt caagcccgaa ggggccgagc
     4141 gtcaattgcc gggcgtcagc cccgttgcca tccaacaggg ccgcttcgta gccaaggaga
     4201 tcgcgcgccg catcgaaggg catacggcac gtcagttcgt gtatcgggac aagggctcga
     4261 tggcaaccat cggccgtagc cgcgccgtcg ccgagatggg cgggctcaag ctcagcggtt
     4321 tcgtggcgtg gctattctgg ctgctggttc acatctatta tctgatcgac ttcaagaacc
     4381 gactcatcgt gctgatcaac tgggcctggg cgtacgttgc gtacaatcgt ggggctcgac
     4441 tgatcacggg tcgcgaggag aagccttgag aggctcgggt accgggcgtg agcccgt
//
LOCUS       DASTKU010000938         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_183283, whole genome shotgun sequence.
ACCESSION   DASTKU010000938 DASTKU010000000
VERSION     DASTKU010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183283"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VFQ61_33145"
     CDS             <1..1192
                     /locus_tag="VFQ61_33145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959400.1"
                     /translation="IGGVQTDSVGGTTYGGTGVYGARIGGVWDALLGEGRNFWFFASS
                     DWHNRGTFGPDDRRSTQDFYPGEYQRNYTLVRSSGAKPRPQQIVDGLRSGNNFATSGQ
                     IIDRLAFVACVGDPGANGRADALVGELAASAAFGNTQLDAAVGCATMGEKLVVPPGSD
                     VVIGIALRDPQGKNHSPYTFPNPSLVQIGVEQPLDEPQLDHVDLIGGLVTGYRTPGTP
                     GYAGEWPRNTNWLRTDGTTSDLSVVPDAAKNVTTSILRTFDGNGASAWKVVTSPEDGT
                     QFVSMTYRIVGVSESRYFRVRGTNLPPAVPYETDPSGNPLADVFTNANDPAMLRIPCN
                     TAHSPKSQFDGCPDHLATAGGASPIAGKKAVSFDVAAWADLWFYSNPIYVEVVGSTVV
                     AGVK"
     gene            1271..2137
                     /locus_tag="VFQ61_33150"
     CDS             1271..2137
                     /locus_tag="VFQ61_33150"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9959401.1"
                     /translation="MSTPLIENYVSETPLTLQRPTLRWRRWGREPLLHFMVLGGFLFG
                     LDWVLSRRAEAPNTIHVGAEVDRQAIETFRAARNRAPTNEELEALRRVWLDNEVLYRE
                     GLSLQVDKGDPAIRERVIFKTLSIVDSNLKLPRASDELLRAWFEKHRTKYDEPARFDF
                     EEAALSGDNTEAAVRGFVAALNAGTPGDAQAGLRVFKARPRANLVDSYGPEFVHALET
                     ARPGIWLAMSTREGWRAVRLNASSPERPAVFEAVRGVVLHDWTDATASEQRTAAVRAI
                     AQKYQVKYEAQP"
     gene            2121..>2852
                     /locus_tag="VFQ61_33155"
     CDS             2121..>2852
                     /locus_tag="VFQ61_33155"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HET9959402.1"
                     /translation="MKPSRSWQWREQSPAPLRRVLQLMLCVFALITLRSAPGIAHEMS
                     MAEMEVRETAPGEFLWQWSAGSDKRVMGRDLEPRWPSRCEANANTLHCGSAGLSGTLG
                     MEGVGKRYSAAMVKVVWLDGQSRVYTLTAAQPSVELYGSAGDRRGLKAIAWAYAVLGV
                     QHILEGIDHLLFVAGLLFLVGFRRNLIGTISAFTLAHSITLASSVFGWIALRSAPVEA
                     SIALSIVLVATESLRDRDTLARRMPA"
BASE COUNT          547 a          885 c          901 g          519 t
ORIGIN      
        1 catcgggggc gtgcaaacgg acagcgttgg cggcaccact tacggcggca ccggtgtata
       61 cggcgcgcga atcggtggcg tctgggacgc gctgctcggc gaagggcgta acttctggtt
      121 cttcgcgagc tccgactggc ataaccgggg cactttcggc ccggatgatc gtcgttccac
      181 ccaggacttc tatccaggcg agtaccaacg caactacaca ctggtgcgaa gcagcggcgc
      241 caaaccgcgg cctcagcaga tcgtggatgg cttgcgcagc ggcaacaact tcgcaaccag
      301 cgggcaaatc atcgatcgct tggcgttcgt tgcttgcgtc ggagatccgg gagccaatgg
      361 tcgtgctgac gcgctggtgg gcgagcttgc cgccagcgcc gcgtttggca acactcagct
      421 cgatgcggcg gtgggttgcg cgacgatggg tgaaaagctg gtcgtaccgc cgggctcgga
      481 tgtggtgatt gggatcgcgc tccgcgatcc gcaggggaag aatcactcgc catatacctt
      541 cccgaatccg tcgctcgtcc aaatcggagt cgaacaaccg ctcgacgaac cgcagctcga
      601 tcacgtcgac ttgatcgggg gcttagtgac cggatataga acccccggaa cgccgggcta
      661 cgccggtgag tggccgcgta acaccaattg gctccgtaca gacgggacca cgagcgacct
      721 ctcggtcgta cccgacgctg ccaagaacgt gaccacctcc attttgcgca cgttcgacgg
      781 caacggtgcg tccgcctgga aggtcgtcac ctcgcccgaa gatggcactc aattcgtcag
      841 catgacttat cgaatcgttg gagtctccga atcccgctac ttccgagtgc gggggaccaa
      901 cttgccaccc gccgtgccgt acgagacgga tccgagcggc aatccgctcg ccgacgtctt
      961 cacgaacgcc aacgaccccg caatgctgcg gatcccatgc aacacggctc acagcccgaa
     1021 gagccaattc gacggctgcc ccgatcacct cgcaaccgcc ggtggcgcga gccccatcgc
     1081 cggcaagaag gcagtctcct tcgacgtcgc ggcctgggcc gacctgtggt tttacagcaa
     1141 tccgatctac gtcgaagtcg tcggatcgac cgtcgtcgcc ggtgtgaaat agtcgcgagc
     1201 gcgggattgg tttgcggcgc ctgcccaagg ggggggcaaa ccgtctcgtg catgaacgga
     1261 tgccattacc atgtcgactc ccctgatcga aaactacgtt tcggagacac ctctcactct
     1321 gcagagaccg actctacgtt ggcgccgttg ggggcgtgag ccgctgctgc acttcatggt
     1381 tttgggtggc ttcctctttg gtttggattg ggtgctttca cgacgagccg aggcgcccaa
     1441 taccatccac gtaggagcgg aggtagaccg acaggcaatc gagaccttcc gcgccgcgcg
     1501 caatcgagca ccaaccaacg aagagctcga ggcgctgcgc cgggtctggc tggacaacga
     1561 ggtcctctat cgagagggcc tttcccttca agtcgacaaa ggggatcccg cgattcgcga
     1621 gcgcgtcatc ttcaagacac tcagcatcgt ggatagcaac ctcaagctgc cgcgcgcgag
     1681 cgacgagcta ttgcgagcct ggttcgaaaa gcatcggacg aagtacgacg agccagcgcg
     1741 cttcgatttc gaggaagcgg ctctatccgg ggacaacacc gaagcggccg tacgaggctt
     1801 cgtggccgcc ttgaatgcag gcacaccggg cgacgcgcag gccgggctgc gcgtgttcaa
     1861 agcacgacct cgcgccaact tggtcgacag ctacgggcct gagttcgtgc acgccctgga
     1921 aaccgcacgc cctggaatct ggctggccat gagcacccgc gaaggctggc gagcggtgcg
     1981 cctcaacgcg agttcgccag agagaccggc ggtattcgag gccgtgcgag gcgtggtttt
     2041 gcacgactgg acggacgcta cggcttccga gcagcggacc gcggcggtac gcgcaatcgc
     2101 tcaaaagtac caggtgaagt atgaagccca gccgtagttg gcagtggcgc gagcaatccc
     2161 ccgctccgct gcgtcgtgtc ttgcaactca tgctctgcgt gtttgctcta atcacgctgc
     2221 gcagcgctcc gggcatcgca catgaaatga gcatggcgga aatggaagtt cgtgagaccg
     2281 cgccgggcga gttcttgtgg cagtggtcag ccggcagcga caagcgcgtg atgggccgcg
     2341 atctggagcc gcgttggcca agccgctgtg aggctaacgc caatactctg cattgcggca
     2401 gcgccggctt gagcggcaca ctcggcatgg agggcgttgg caaacgctac tccgcggcca
     2461 tggtcaaagt agtatggctc gacgggcaga gccgagtcta tacgctcacc gccgcccaac
     2521 ccagcgtgga gctctacggt tcagcgggcg atcgacgagg actcaaggcg atcgcttggg
     2581 cctatgcggt gctcggggtt cagcacatct tggaaggcat cgatcatttg ttgttcgtgg
     2641 cgggcctgct gttcttggtc ggatttcgac gtaacctaat tggcacgatc agcgcattta
     2701 ctctcgcgca cagcatcacg ctcgcgagca gcgtattcgg ctggatcgcc ttgcggtccg
     2761 caccggtgga agcctcgatt gcattgtcca tcgtgctcgt ggcaaccgag tcactccgag
     2821 acagagatac gctggcgcgc cgcatgcccg cc
//
LOCUS       DASTKU010000939        16413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1524759, whole genome shotgun sequence.
ACCESSION   DASTKU010000939 DASTKU010000000
VERSION     DASTKU010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16413
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524759"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..1032
                     /locus_tag="VFQ61_33160"
     CDS             388..1032
                     /locus_tag="VFQ61_33160"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET9959403.1"
                     /translation="MMGRARSYLMLALLVGCHRGSEDAIGAREARSAATPSITSPSPT
                     TSALNPDTAPALATSPPPCVVPFPTDPAPQAKPAAECPKDPVVRPPTLATGDVVFQDA
                     PGMPRIHVEIADTPDTQQRGLMYRTTMPEDHGMLFEWPYELTRTFWMQNTCIPLDMLF
                     IAKDGTIGGILEQVPVLNEAPRSIPCKASYVLELNAGASRRLGLQAGMRVRVEK"
     gene            complement(1084..1377)
                     /locus_tag="VFQ61_33165"
     CDS             complement(1084..1377)
                     /locus_tag="VFQ61_33165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959404.1"
                     /translation="MATTLRCPFGIATGGVAGAAEPEATGAPLALGAAGDADGAALLT
                     AAGSAAGVVAGAGVLGGGVVGVALVSGLTRLSGSGGVVDRHCVSSGKANASKT"
     gene            1370..1981
                     /locus_tag="VFQ61_33170"
     CDS             1370..1981
                     /locus_tag="VFQ61_33170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9959405.1"
                     /translation="MAKPVKEVPPSADDPVHGQFSLEDALKGLTGKGRLVAEIRTDLG
                     KLECELYEDKAPITVANFVGLARGLRPFKNPKGEWGKSHAYDGTTFHRVIKGFMIQGG
                     DPAGTGGGEPGYLVPDEIWEDAYHSEQGLLCMANRGPNTNGMQFFIMDGSAPHLDNGY
                     TIFGKCGPGDVITKLASVEVQGDRAVKPPKIEKVTIKRVAPKK"
     gene            complement(2146..3408)
                     /locus_tag="VFQ61_33175"
     CDS             complement(2146..3408)
                     /locus_tag="VFQ61_33175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959406.1"
                     /translation="MNLRAALALYALFTTVAFALASPELFRQHTHANHFALLAEAWLQ
                     GRLDLPGPPPAYTGNDDFAYFEGRHFIVFPPFPALLVLPLVGLLGSAARLPDGAFFLG
                     CAGAAPALLFMALQKLSAVGISARSQRENWVIAALFGFGSVYFFSAVQGTVWFAAHVV
                     GTSLAAGFLYCAIGAEHPIWAGVLLGLAGATRTPLFFAAPFFFFECLRGRSWRELGSN
                     RELRSRGLAFCLPLLGVGLLLALHNLARFHDPLEFGYRYLTVVWRARIERYGLFSFHY
                     LPRNLGVLLTSLPWWHQPRAPWVINLHGLALWFTTPLYLLLPWSQVHRSEAARGLAWT
                     ALCVALPSLFYQNTGWLQFGYRFSNDYAPFLFAWFAVGGLRLRGVALAFAIWGVCVNA
                     LGALTFGRPEYSRFYFAERTQTVLYEPD"
     gene            3695..5422
                     /locus_tag="VFQ61_33180"
     CDS             3695..5422
                     /locus_tag="VFQ61_33180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959407.1"
                     /translation="MAERIKVLLKRVFVKNDADWFGSGEFYFTCDVDGRRVGNREQIF
                     TARTREWIVLPAAAWSAVVDVTNKAQVVVHFNGKDDDLFWDDDLGTVAYTLRPPWAER
                     SFRSETEFFILEWGVELEVDGAFGHHPPNEVFACRSAAGNATCTTVSGVPITARIEIH
                     PVRPVPASLPPRGLPAGPVLGVLNDQGTTISPGDPINIVPNPSVIPILTAPGGAAPAA
                     GAPPVADATNCARIEVTFYRPNTLAFTDQDPRLVWSFRALAGGAVSFLGAPHGLKVMV
                     YGTAAGEVLLELRFRGELMAAYRALVMPVKQIPCRCNILNGPNASSRPRATPANVEDH
                     LKIANRYLRQIALELVLDTNAHRSHGATATGTPGIFRIAVSKGTTWHINSDLTAVPAT
                     GLNYRPNVMNFAYIHSERRPSIGGAATDFPSSTVAPPGPGARPRITDTGTPSSSWIRP
                     SGVLPDGAAAAVSMDLINGIQRPGHPRLFAMFLCDNIGDPAVLADQQLYAKSMCHELG
                     HILNLGHRVEGPDATTATGLVANGMFWDGLSHPPNQNVMFWQGVQDICQDFDIVQARA
                     VHRSPLVPP"
     gene            5419..6606
                     /locus_tag="VFQ61_33185"
     CDS             5419..6606
                     /locus_tag="VFQ61_33185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959408.1"
                     /translation="MRRVETALLRARLRHDPPCPSPAGLVSPTARARKLASSWLLCAM
                     SCALACTALRAPSGERAGCACHRAEPGATTVVDSAPADLGLASTASGRSRRPALAASS
                     ASAASTPPASTPPASTLPSPPSATSATAVNPPAHPLFDEALQAARGVDPEGHARLRAQ
                     LQSRSFLDALDTAEDYANASRVHLRFERVATALAENLAPSARKAFLRLLESPLLLNDD
                     ERALALVRAGVGWRPAPAELIAFWNSHSRANDGFTPTTITVLVENGSAPALALLGRKL
                     LDVSHSSDEKLAWLRSDVLTHRNDLGLLQLCRKLLLGGWSAEMKAALVEVVFDYQPQA
                     WYRPATVHSPPPLSAAAAPALVELESLGQLALKQIPLNSTQRAAVAHRLNEVRALSAH
                     NEP"
     gene            6603..7418
                     /locus_tag="VFQ61_33190"
     CDS             6603..7418
                     /locus_tag="VFQ61_33190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959409.1"
                     /translation="MTNAETDPKQMLRSALTLARGQEPAAHDELLLSLQSLEFLERLD
                     SPAEYQMASKYRLRVTQILEALARNPAPTARHVLVELTASDVFLAHDERIIVLIRASV
                     FLRPPAPPVVDFWDRHSQPDDGFTPTTIPVLVENGSPPALNLLESKLADPAHEDQDKI
                     SWFRTTILCHRNDLELLLMCERALGHGLPASLRPALVEVLFDYRPEEWFRPVSSYSPP
                     PLANASTAALLRLQQLGFYALQSVNLDSNQRQAVGVRLREAQAELAARPQPPP"
     gene            complement(7458..8069)
                     /locus_tag="VFQ61_33195"
     CDS             complement(7458..8069)
                     /locus_tag="VFQ61_33195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959410.1"
                     /translation="MRSPQSRIRPFDTRGPYGASWELHARGMLSRERYAQRVHPLVAS
                     RWVEAAVSDPFLWSMMLGWYERLTGLRSAPPTSTLVRREIVPRLQRAFRDETWVLVPR
                     EEPGPPEAIAATGPTQAASPAKAASAPRRVLSEEDYSFVEFKLVDEQGQPIAGLAYEI
                     RLPDGRKAQGSTDARGSVRFDRIPFGDCSISFPSLDSAAVVSA"
     gene            8258..9001
                     /locus_tag="VFQ61_33200"
     CDS             8258..9001
                     /locus_tag="VFQ61_33200"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HET9959411.1"
                     /translation="MIVATTHRVSAGECILTLSEAYGVPVDKIWNHPQNSDLKRRRGN
                     PNVLKEGDQVFVPDVTKFALTRSTGGVHEIVIKRPTAKLKLRVVVDPGQSGAEPEAAP
                     APANRKVSVGEDPEPSREARHDEPRANVKYVVELDEVRISGVTDGDGVLECDIPTKAR
                     SGRLILAPGTPDETVLPLNLGHLDPIDTVSGVKQRLANLCFDCGDRSDEETPGFASAL
                     RAFQTKQGLAPTGQLTSETRHALQTAHGS"
     gene            9179..9925
                     /locus_tag="VFQ61_33205"
     CDS             9179..9925
                     /locus_tag="VFQ61_33205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959412.1"
                     /translation="MSKPEFVVPVADLEAGPRQVSWAIPLAWLEQTFEGTQATARAPG
                     RLEVELSKSGREVMVRGRVEVQVTMPCVVTLDPLDFDLQPEVFLMLSPAKEPHGGGRE
                     RQKGRVAGASGPGAPTPRGEQGAALRVVKGGKAAGPTTAKSAARPGKPGNERPGKPAS
                     GWADDPELSESDAARDTFDGERVVLDDFVREFLVLELPMYPRRSDLPREESAAIAPAS
                     PSSGSASSSIDPRLLPLAELANRLREKKKE"
     gene            9932..10123
                     /gene="rpmF"
                     /locus_tag="VFQ61_33210"
     CDS             9932..10123
                     /gene="rpmF"
                     /locus_tag="VFQ61_33210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET9959413.1"
                     /translation="MAVPKRRLSSSKRDMRRAQHDKVTAPNIVPCPNCSAPMVPHRIC
                     PSCGFYKGKAVLKEEETSK"
     gene            10407..11402
                     /locus_tag="VFQ61_33215"
     CDS             10407..11402
                     /locus_tag="VFQ61_33215"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET9959414.1"
                     /translation="MFSEPRLRSRIVGTGAYAPARVLTNFDLEKMVDTSDAWIRERSG
                     IRERRIASEGEASSDMAMAAARSALEMAALSANDLDMIIVGTVTGDMPMPACAAFLQR
                     KLGVTDIPAFDVSAACAGFLYGLTIADQFLRTGTHRTILVVGVELLSRIIDWTDRTTC
                     VLFGDGAGAAILRAEPGERGVLSTQLHTDSTYVESLCIPGGGSRERLSPDGLARKRDK
                     VFMNGQDIFKVAVKNLTRASSSALDQAGLKPSQVNWVVPHQANLRILTQVAQRLEIPM
                     DRFVINIDRYGNTSSASIPIALDEAVRDGRIRDGDTVLMCALGGGISWGSALLKM"
     gene            11429..12364
                     /gene="fabD"
                     /locus_tag="VFQ61_33220"
     CDS             11429..12364
                     /gene="fabD"
                     /locus_tag="VFQ61_33220"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET9959415.1"
                     /translation="MKIAWLFPGQGSQEVGMGRALFEASPAAREVFESADRALGRSIS
                     RICFEGPVTDLTLTENTQPALVATSSAILAALRERYPSLPAPVFAAGHSLGEYSALVA
                     AGALSLQDAVRLVELRGRAMQSAVPEGKGAMVAVVGGDREAVLALCEEAKSAGVVEPA
                     NFNAPGQVVLSGEADAVAKVSALAAERSLKAIPLKVSAPFHCSLMTPAAEAVARALET
                     IEVRPAAFPIIANASASPNQEPSQTKRLLVEQIAGPVRWDQSISHMAEAGVTHALEIG
                     PQKVLANLVKRIDKRIRVLSLSDPDALAKVSEFLG"
     gene            12510..13256
                     /locus_tag="VFQ61_33225"
     CDS             12510..13256
                     /locus_tag="VFQ61_33225"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET9959416.1"
                     /translation="MFDLSAKVALVTGGSRGIGRSVGETLAARGATVVLGYASGKDAA
                     ESAAAAIRDRGGKAEALALDVTQVEATEKAIAETAKRLGRLDILVANAGIAIDGLLLR
                     LNEDDFERTLAVNLKGAVACARAAVKVMMRAKTGRIIFISSVVGEMGNAGQTAYAASK
                     AALLGVTKSIAREYASRNITVNAVTPGFIDTDMTGSLTDVQKASIASAIPLGRTGTPL
                     EVAAAVTFLASDEAAYVTGHALRVNGGMYM"
     gene            13342..13590
                     /gene="acpP"
                     /locus_tag="VFQ61_33230"
     CDS             13342..13590
                     /gene="acpP"
                     /locus_tag="VFQ61_33230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9959417.1"
                     /translation="MAEGRDITAEVKRIIKEQLDVDEKDIKPESTFIDDLGADSLGLV
                     ELVLAFEEAFEIDIPDEDTEKIRTVQDAVDYIEKHLNS"
     gene            13829..15076
                     /gene="fabF"
                     /locus_tag="VFQ61_33235"
     CDS             13829..15076
                     /gene="fabF"
                     /locus_tag="VFQ61_33235"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET9959418.1"
                     /translation="MERVVVTGIGLVTPSGVGTEETWQSLIRGKSGVGPITLFPAGPE
                     YATRIAAEVKNFDPAAFMEKKKLKEVSRFIPFAMGATKMALEDANLELTDEERERAGV
                     FIGVGLGGLENLERCTLTLAQKGPGKVSPYFIPSLIANMAAGQVSIAFGFRGPSLAHT
                     SACSSSAHALGEALRWMQRGDAEVMIAGGAEATITPIGIGGFSAMFALSRRNDEPERA
                     SRPWDRGRDGFVCGEGAGTLVLETLTRAKRRGARIYAELVGYGATSDAHHITKPAANG
                     AGGARAMRMALKDAKLAPEAIDYINAHGTSTPAGDLEEAKAIADVFGAHATDKKLWVS
                     STKSMMGHLLGAAGAVEAAVCIKAMGSSLVPPTINLEDPDPEAGLDFVPNQTRERRIR
                     HAMSNSFGFGGTNATVVFSAFEG"
     gene            15089..15565
                     /gene="nrdR"
                     /locus_tag="VFQ61_33240"
     CDS             15089..15565
                     /gene="nrdR"
                     /locus_tag="VFQ61_33240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET9959419.1"
                     /translation="MQCPFCREMDSRVVDSRLVAGGTAIWRRRACDACNRRFTTYERV
                     EYQLPMVVKKDGQREPFSRDKLLSSLRIACNKRPVATDALEEHAAQLERELAESGEKE
                     ISTRVIGERVMERLKQLDGVAYVRFASVYRSFRDIDEFMAEMGKLVRSRGAGDAPE"
     gene            15681..>16413
                     /gene="ribD"
                     /locus_tag="VFQ61_33245"
     CDS             15681..>16413
                     /gene="ribD"
                     /locus_tag="VFQ61_33245"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET9959420.1"
                     /translation="MARALELGRAGDPSPNPHVGCVIADNERIVAEGYHTAAGHDHAE
                     IVALKKAGSNARGCTLYVTLEPCNHAGRTPPCVDAIAAAGIRRVVIGTRDPNPRVVGG
                     GIERLRTLGIEVTEGVLESEALELIKPWTKFVNQDSSYLALKLALSLDGRIATRSGAS
                     KWITCPESRLRVQTLRAHHDAVMVGVNTVLTDNPRLTVRDVPGRSPVRVVVDSRLRIP
                     LDCQVVATAREIPTCVATTPQAPRAS"
BASE COUNT         3162 a         5353 c         5176 g         2722 t
ORIGIN      
        1 cgccggaacc cgacgctgcg gagaatcccc cactcggcgt gctgttgctt ccgctaccac
       61 ctcccgcgtg aacgccgcct tcgcccgaat cggcttggcc accgcacgcc aacaatcccc
      121 acaccgagag gcaagctaaa acggcagaac ggcaagccgc ggaacagcag agcgcggacc
      181 aatagcgcgc ggaccaatag cgcgccgtga gtctggacga gcgagtgcac cacccatgca
      241 tggcgaaggc acagcatagc cgctcccccg gcagagtccc gctcgtgtag ctcagtttct
      301 gggagccccc aattttctgc ctggccccgg atacgccccg ggagaagggt cacccaagcc
      361 gtcgtccgcg tgctaaacgg gcgcatcatg atgggtcgag ctcgtagcta tctcatgctg
      421 gcgctgctcg tgggctgtca ccgcggctca gaagacgcga tcggcgcccg cgaagcccgg
      481 tctgccgcga ccccgagcat cacttcgccc tctccgacga ccagcgcact caacccggac
      541 acggcgcccg ccctcgccac gagcccccca ccctgcgtgg tcccgtttcc aaccgatccc
      601 gccccccagg caaaacccgc ggcagagtgt ccgaaggacc cagtggtgcg cccgcccacg
      661 ctcgcgacgg gagacgtcgt ttttcaggat gcaccaggca tgccccgcat acacgtcgag
      721 atcgcggaca ctcccgacac gcagcagcgc ggcctaatgt accggacgac catgcccgaa
      781 gatcacggca tgttgttcga gtggccttac gagctcacgc gcaccttctg gatgcaaaac
      841 acctgtatcc cgctcgacat gctgttcatc gcaaaagacg gcacgatcgg cggaattcta
      901 gagcaagtgc cggtgctgaa cgaggccccg cgcagtattc cctgcaaagc tagttacgtg
      961 ctcgagctga acgcgggagc cagccgacgc ttggggctcc aggccggcat gcgcgttcga
     1021 gtcgagaagt aagcccgaca accgatcgat taaactccga gggaccagca acatgcgttc
     1081 gaactacgtc ttactcgcgt tggctttacc gctgctcacg cagtgtcgat cgacgacgcc
     1141 gcccgagccc gaaagccgcg tcagccccga aaccaacgcc acaccgacca cacctccacc
     1201 cagcacgcct gcgcccgcca ctactccagc ggcggaacct gctgcggtca acagtgctgc
     1261 gccatccgca tctcccgcag cccccagcgc gagcggtgca cccgtagctt cgggttccgc
     1321 cgcacccgcg acgccgccgg ttgcgatccc gaaggggcac cgcaaagtcg tggccaagcc
     1381 ggtcaaagaa gttccgccga gcgcggacga tcccgtgcat ggtcaattca gcctggagga
     1441 tgcgctcaag ggcctcactg gcaaaggccg cctcgtcgcg gaaatccgca ccgacctcgg
     1501 caagctcgag tgcgagctat acgaagacaa agcccccatc accgtggcta acttcgtggg
     1561 cttggcgcgt ggcctgcgcc cgttcaaaaa tcccaaaggc gagtggggca agagccacgc
     1621 ctatgacggc accaccttcc accgcgtgat caaaggcttc atgatccaag gcggtgatcc
     1681 tgccggcacg ggcggcggcg agccgggtta cctggtgccc gacgaaatct gggaggacgc
     1741 ataccactcc gagcaaggcc tactctgcat ggcaaaccgc ggcccgaaca cgaacggcat
     1801 gcagttcttc atcatggacg ggtcggcacc gcatctggac aacggctaca caatcttcgg
     1861 caagtgcggc ccgggtgacg tcatcaccaa gctcgccagc gtcgaagtgc aaggcgatcg
     1921 cgccgtgaag ccgcccaaga tcgagaaggt cacgatcaag cgcgtagctc ccaagaagtg
     1981 acctccgcgg ccacttagct ccgcggccac ttagctccgc gggcccgtct agcaccgcgg
     2041 ccacttagca acgggcgcgc cgcgcgtcgc ctcacccgca ggcgcagcct aatcgcgcgt
     2101 agcgcgcccc ttcaccgcgc cggccccctc agcaacctga cggcgtcaat ctggctcgta
     2161 caacaccgtc tgagtccgtt cggcgaagta aaaacgcgag tactcggggc gcccgaaagt
     2221 gagcgccccc aatgcgttga cgcatactcc ccagatcgca aaagccaacg caacaccgcg
     2281 taaccgcagc cctccgaccg caaaccacgc gaacaggaac ggcgcgtagt cgttggaaaa
     2341 acgataaccg aactgcaacc accccgtgtt ctgataaaag agcgatggca acgccacgca
     2401 aagcgcggtc cacgccaagc cgcgcgccgc ttcgcttctg tgaacttgcg accacggcaa
     2461 caatagatag agcggggtcg taaaccacaa cgctagtccg tgcaaattga tgacccacgg
     2521 tgcacgcggt tggtgccacc acggcaggct cgtcagcaga acacctagat ttcgaggcag
     2581 gtaatgaaag ctgaagagcc catagcgctc gatgcgtgct cgccaaacca ccgtcaagta
     2641 ccggtagcca aactcgaggg gatcatggaa acgagccaag ttgtggagcg cgagcaacag
     2701 gcccaccccg agcagcggta ggcagaaggc caagccgcgg cttcgcagct cccgattcga
     2761 gccgagctcg cgccaagagc ggcctctcag gcactcgaag aagaagaacg gcgccgcaaa
     2821 aaacaacggg gtacgcgtcg cgcccgccag cccgagcagc acacccgccc agatggggtg
     2881 ctccgcacca atcgcgcaat acagaaagcc cgcagccagg ctcgtgccca ccacgtgtgc
     2941 ggcgaaccac acggtgccct gcaccgcact gaagaagtag acgctaccga acccgaacag
     3001 cgccgcgata acccagttct cgcgctggct ccgcgcgctg atccccaccg cgctcagctt
     3061 ctgcagtgcc ataaagagca acgctggcgc cgctccggcg caacccagaa agaatgctcc
     3121 gtccggcaag cgcgccgcgc tgccgagtag gccgaccagc ggtagcacca acagcgccgg
     3181 aaacggcgga aagactatga agtggcggcc ttcgaagtaa gcgaaatcgt cgttgccagt
     3241 atacgcgggc ggtggcccgg gcaagtcgag tcgcccttgc agccaagcct ctgccaatag
     3301 cgcgaagtgg tttgcgtgcg tgtgctgtcg gaatagctcg ggcgaagcga gagcgaaggc
     3361 taccgtcgtg aagagcgcat acagagccag ggcagcccgc aggttcatgg gggggcgcag
     3421 aatagggaca ggtaagccgc aaagtcgacg gttcggcgtt cccgggcacg aaagcccacc
     3481 cacggccgct cggcaccggt tcggtcgaac gacgcgagta gaacaaatga caacgccgac
     3541 gacccgctcg atgaaacccg cgtgcccgac ccacctctcg attcgtcgac caccagcgga
     3601 tcgggcgcga acccaagagc tcgcgcctgg tgcctaaacg aaatgatgta agctgaaggt
     3661 tcgaggcgga ccgcgcctca agctggagag cggcttggca gaacgcatca aggtgctgtt
     3721 gaagcgggta ttcgtgaaga acgacgcgga ctggttcggc tccggtgagt tctacttcac
     3781 gtgcgacgta gacggccgga gagtgggaaa ccgggagcaa atcttcaccg cgcgaacgcg
     3841 cgaatggatc gtgcttcccg cggcggcgtg gtccgcggtc gtcgacgtta cgaacaaggc
     3901 gcaagtcgtg gtccacttca acgggaaaga cgacgacttg ttctgggacg atgacctcgg
     3961 cactgtcgcc tacacgctac gtccgccgtg ggccgagcgt agcttccgct ccgaaacgga
     4021 gttcttcatc ttggagtggg gcgttgaatt ggaggtggat ggcgcgttcg gccatcaccc
     4081 gccgaacgag gtgttcgcct gtcgaagtgc ggcgggaaat gcgacctgca cgaccgtttc
     4141 aggcgtgccg attaccgctc ggatcgagat tcaccccgtg cgcccggtgc cagcgagtct
     4201 gccaccgcgc gggctgccgg cgggccccgt gttgggtgtg ttgaatgatc aagggaccac
     4261 gatttctcct ggcgacccga tcaatatcgt tccgaacccg tccgtgattc cgatcctcac
     4321 ggccccgggc ggggccgctc ctgcggcggg ggcaccgccg gtcgccgatg ccacgaactg
     4381 cgcgcggatc gaggtcacgt tctatcgacc gaacaccctc gccttcacgg atcaggaccc
     4441 gcggttggtg tggtcgttcc gcgcgcttgc cggtggcgcg gtcagcttct tgggagcgcc
     4501 ccatgggctc aaggtgatgg tttacggcac ggccgcgggt gaggtgctgc tcgagttgcg
     4561 ctttcgaggc gagctgatgg ccgcgtaccg cgcgctcgtc atgcccgtga agcagattcc
     4621 ctgtcgttgc aacattttga atggccccaa cgcgagcagt cgcccgcgcg cgaccccggc
     4681 gaacgtggag gaccatctca agattgccaa ccgctacctc aggcaaattg cgctggagct
     4741 cgtgctcgac accaacgctc atcgctcgca cggcgccacc gctacgggca cgccgggaat
     4801 ctttcgcatc gcagtctcga aagggacaac ctggcacatc aactcggatc tcaccgcagt
     4861 gcccgcgacg ggcttgaact accgcccgaa cgtcatgaat ttcgcgtaca tccactctga
     4921 gcgacgaccc tcgataggtg gcgcagccac ggatttcccg tcgagcaccg tcgcgccgcc
     4981 cgggcccggt gcacgccctc ggatcactga taccggtacc ccgagcagct cgtggatacg
     5041 tccgagcgga gtcttgccgg atggcgccgc ggccgccgtg agcatggacc taatcaacgg
     5101 gatacaacgg ccaggtcacc cacgtctgtt cgcgatgttc ctgtgcgaca acatcggtga
     5161 cccggcagtg ctcgccgatc agcagctcta cgccaagtcc atgtgccacg agctcgggca
     5221 cattctgaac ctcggtcatc gagtagaggg gccggacgcg acgaccgcca cggggctcgt
     5281 tgccaacggg atgttctggg atggcctcag tcatcccccc aatcagaacg tgatgttttg
     5341 gcagggtgtg caggacatct gtcaagattt cgatatcgtg caggcgcgcg ccgttcatcg
     5401 cagcccattg gtgcccccgt gaggcgagtc gaaaccgcac tgctacgcgc ccgcttgcgg
     5461 cacgatccgc cctgtccgtc accagccggc ctggtttcgc ctacagcgcg cgcgcgcaag
     5521 ctcgcgagca gctggctgct ctgcgcgatg tcgtgcgcgc tcgcgtgcac ggctctgcgc
     5581 gcacccagcg gggaacgcgc cgggtgcgct tgtcatcgcg cggagcctgg agccactacg
     5641 gtggtcgatt ccgcccccgc cgaccttggg cttgcttcta cggcgagcgg tcgctcgcgc
     5701 cgccccgctc tcgcggcttc ctcggcatct gcggcttcga ctcccccggc ttccactccg
     5761 ccagcttcga ctctgccgtc gcccccttca gcaacgagtg cgactgcggt caatcctccc
     5821 gctcatccgc tgttcgatga agcactgcag gccgctcgcg gcgttgaccc cgagggtcat
     5881 gctcgcttga gagcgcagct ccaatcccgc tcgttcctcg acgcgctcga caccgcggaa
     5941 gattacgcga acgcgtccag ggtacacttg cgattcgagc gcgtggccac cgcgctggca
     6001 gagaatctcg ccccgagcgc gcgcaaagcc ttcctgcggc tgctcgagag cccgctgctc
     6061 ctgaatgacg atgaacgcgc actcgctttg gtgcgcgccg gcgtcggctg gcggcccgcc
     6121 cccgccgagc tgatcgcctt ctggaactcg cacagccgcg ccaacgacgg gtttacaccg
     6181 accacgatca cggtgctggt cgagaacgga agcgcccccg cgttggcact gctggggcga
     6241 aaattgctcg atgtcagcca cagctcggat gagaagctag cttggctgcg cagcgacgtg
     6301 ctcacccacc gtaacgatct cgggcttctg caattgtgtc gcaagctgtt gcttggcggc
     6361 tggtccgccg agatgaaggc ggcgctggtc gaagtcgtgt tcgattatca gccccaagct
     6421 tggtaccgac cggcgacggt ccattcgccg ccacccctga gtgcagccgc ggctccggcg
     6481 ctagtagagc tcgagtcttt ggggcagttg gcgttgaagc aaattccact gaactccacg
     6541 cagcgggccg cagttgccca tcggctaaac gaggtgcgcg cgctctcggc gcacaacgaa
     6601 ccatgacgaa cgcggaaacg gatcccaagc aaatgctgcg ctcggccctg acactcgctc
     6661 gcggccagga gccggctgcc cacgacgagt tgttgctgtc cctgcagtct ctggagtttc
     6721 tcgagcgcct cgattcgcca gcggagtacc agatggcctc gaagtaccgg ctgcgggtga
     6781 ctcaaatcct agaagcgctc gcgcgcaacc ccgcgccgac agcgcggcac gttctggtcg
     6841 agctcaccgc gagcgacgta tttctggctc atgacgagcg catcatcgtg ctgatccggg
     6901 ccagcgtttt cctgcgaccc ccagctccgc cggtcgtgga cttctgggat cgtcacagcc
     6961 aacccgatga cggcttcaca ccgaccacca tccccgtgct cgtggagaac ggctcaccgc
     7021 cggcgttgaa cctgctcgaa tcgaagttgg cagatcccgc tcacgaggac caagacaaga
     7081 tctcctggtt ccgtacgacc attctgtgtc atcgcaacga cctggagctg ttgctgatgt
     7141 gcgaacgtgc gctgggccac ggcctccctg cttcgctacg cccagcgcta gtcgaggtct
     7201 tgttcgacta ccgtcccgaa gagtggtttc gccccgtctc gagctactcg ccgcccccgc
     7261 tcgcgaacgc ctcgaccgcg gcgctgcttc ggttgcagca gctggggttc tacgcgctgc
     7321 aatccgtgaa cctcgactcc aatcaacgcc aagcggtagg tgttcggctg cgtgaagcgc
     7381 aagccgagct tgcggcgcgg cctcagcccc cgccctgagc acgcacgagc gccaccatta
     7441 ccgatcagcg acacgagtca agcggacacc acggccgcgc tgtcgagtga ggggaacgaa
     7501 atcgagcagt cgccgaacgg gatgcgatcg aagcgcacac tgcctcgagc gtcggtgctg
     7561 ccctgagcct tacgtccatc gggcaatcgg atttcgtagg ccaaccctgc gatgggctgc
     7621 ccttgctcgt ccaccaactt gaactcgacg aaggagtagt cctcctcgct caagactcga
     7681 cgcggtgcgg atgcggcttt tgcaggcgat gcagcctggg tcgggccagt cgcggcaatc
     7741 gcctcgggcg gcccgggttc ttcgcgcgga acgagcaccc acgtctcgtc acgaaaggct
     7801 cgctgcaggc gcggcacaat ctctcgtcgc accagcgtcg aagtgggcgg cgccgagcgc
     7861 aagccagtca gccgctcata ccagccgagc atcatcgacc atagaaacgg atccgagacg
     7921 gcagcttcca cccaacggct ggcgaccaac ggatgcacac gctgcgcata gcgctcgcga
     7981 gagagcatac cgcgcgcatg aagctcccac gacgcaccgt aaggacctcg agtgtcgaag
     8041 ggacgaatcc gggactgggg agaacgcata ggaagaacct cgagtgtgga gtcgaggggc
     8101 gagccgcagc ctgcagtcag gcatagcacg cgcccacctc gaactgcctc gcgatgaaag
     8161 agggtgctat taaacagcag cgccgtcggg cagccggcgc agacttgccc acagtacctt
     8221 cgaagagctc gctttcgagc tcagagcgca acgaggcatg atcgtggcga cgacgcatcg
     8281 agtgagcgca ggcgagtgca ttctaaccct ctcggaggct tacggagtcc ccgtcgataa
     8341 gatctggaac cacccccaga actcggatct caagcgtcgg cgcggcaacc ccaacgtgct
     8401 caaggagggc gatcaggtgt tcgtgcccga cgtgacgaag tttgccctca cgcgctcgac
     8461 gggcggtgtg cacgagatcg tgatcaagcg ccccacggcg aagctgaagc tacgggtcgt
     8521 ggtcgatccg gggcaatccg gagctgagcc cgaggccgcg ccagcccccg ccaatcggaa
     8581 ggtttcggtc ggcgaggatc ccgaacccag ccgagaagcc cgacacgacg agccaagggc
     8641 gaacgtgaag tacgtcgtcg agctggatga ggttcgtatc tccggcgtta cggacggcga
     8701 tggcgtgctc gaatgcgaca tccccaccaa agcgcggagc gggcgcttga ttctggcccc
     8761 gggaacgccc gatgagacgg tgttgccgtt gaatctcggg cacctcgacc ccatcgatac
     8821 cgtgagcggc gtcaaacaac ggctcgcgaa tttatgcttc gactgtggtg accgaagcga
     8881 tgaagagacg cccgggtttg cgagcgccct acgcgcgttc caaaccaagc aagggctcgc
     8941 acccactggc caactcacga gcgagacgcg ccatgcccta cagaccgcgc acggttcttg
     9001 agccaatgaa tcgaagctcg gcttccgctc cgctctgaag ccaaggaacc gtttgccacg
     9061 acctcggttg ggcgagcgcc gtacactcat catcgtctcg tatgccccgt gcacaatttg
     9121 gggcgcttgg cgcgagtgcg ctatcgacgt cggccaatcg ggtgctaggg tcccgcgcgt
     9181 gtcgaagcct gagttcgtgg taccagtcgc cgatctggag gcgggtcctc gccaagtcag
     9241 ctgggccatt ccgttggcat ggctggagca aaccttcgag ggcacgcaag ctaccgctcg
     9301 cgcgcccggt cgtctggaag tagagctctc gaagagcgga cgcgaggtca tggtccgcgg
     9361 ccgagtcgag gtgcaggtca ccatgccttg cgtcgtgacg ttggacccct tggatttcga
     9421 tctgcagccg gaagtcttct tgatgctttc tccggccaag gagccgcacg gcggtgggcg
     9481 cgaacgccag aaagggcgcg tggctggggc gagcggtccc ggagctccta ccccacgcgg
     9541 tgagcaggga gccgccttgc gtgtagtcaa gggcggcaaa gcggccggcc cgaccactgc
     9601 caagtcagcg gcaaggcccg gaaaacctgg aaatgagcgc ccgggcaagc cagcgtccgg
     9661 ctgggcggac gatcctgaac tctcggaatc ggacgccgct cgcgacacct tcgacggcga
     9721 acgtgtggtg ctggatgact tcgtgcgcga gttcctagtc ctcgaattac cgatgtaccc
     9781 ccggcgttcg gacttgcctc gagaggaatc tgccgctatc gctcccgcct ccccgagctc
     9841 ggggtccgct tcgagctcga ttgatccccg cctcttgccg ttggcagaac tcgccaatcg
     9901 gctccgtgaa aagaagaagg agtaagccgc cgtggccgta ccgaagcgac gcttaagcag
     9961 cagcaagcgc gacatgcgtc gtgctcaaca cgacaaagtc accgctccca acatcgtgcc
    10021 gtgcccgaac tgctcggcgc cgatggttcc tcaccgcatc tgcccgtcct gcggtttcta
    10081 caagggcaag gcggttctga aggaagaaga gacttcgaag taaagcttcc cgaagtacgc
    10141 aatcgcagta cggcggcagc tcgggcgaat gcgcgtcagg ctcgatcagg atcgagtgag
    10201 gatcagagac tgattcgacg cgctcagccc gagcatcagt ctgagcctgc cgagcggtag
    10261 gcacgttgtc gggctgcgcc gtgagattgc tgtcgtaaga tcgcggtcac gaggttgctg
    10321 tgcagagctg acgtaggggg tggcctgcac actggttcaa ccattttgcg tgacggcgcg
    10381 agcagagcgg cgtaacgggt aaggccatgt tttccgagcc ccgactacgc agtcgcatcg
    10441 tgggtacggg tgcgtacgct cccgcgcgcg tactcaccaa tttcgatctc gaaaagatgg
    10501 tcgatacctc ggacgcctgg atccgagagc ggagcgggat ccgagagcgg cgcatcgcga
    10561 gcgaaggaga agccagcagc gacatggcta tggcagccgc gcgctcggcg ctcgagatgg
    10621 ccgcgctttc cgcgaacgac ctcgacatga tcatcgtcgg aacggttacg ggtgacatgc
    10681 cgatgccggc ctgcgccgcg ttcctgcaac gcaaactcgg cgtcaccgac attcctgcgt
    10741 tcgacgtgtc agcggcgtgc gcaggcttct tgtacggcct cacgatcgcc gatcaatttc
    10801 tacgcaccgg tacgcatcgc acgatcttgg tggtgggcgt ggagctgttg agtcgaatca
    10861 tcgattggac cgatcgcacc acgtgcgtct tgttcggtga cggcgcggga gccgctatct
    10921 tgcgcgccga gccgggcgag cgcggtgtgt tgtccaccca gctgcacacc gactcgacgt
    10981 acgtcgagtc gctctgcatc cccggtggcg gcagtcgtga acgtctcagc ccggacggac
    11041 tcgcgcgcaa gcgcgacaaa gtattcatga atggccagga catcttcaaa gtcgccgtga
    11101 agaacctgac ccgagcctcg agttcggcgc tcgaccaggc tggcctgaaa ccgagccagg
    11161 taaattgggt cgtgccgcat caggccaatc tcagaatttt gacccaggtg gcgcagcgcc
    11221 tcgaaatccc catggatcga ttcgtgatca acatcgatcg ctacggcaac acctccagcg
    11281 cgtcgattcc aatcgcgctc gacgaggccg tgcgcgatgg tcgaattcgc gacggagata
    11341 cggtattgat gtgcgccctc ggcggtggca tctcctgggg cagcgcgctg ctgaagatgt
    11401 gaagcgccgc aaaagtgagt actgaagaat gaagatcgcg tggctttttc cggggcaagg
    11461 cagtcaagaa gtaggcatgg ggcgcgcgtt gttcgaagct tcgcccgccg cgcgggaggt
    11521 cttcgaaagc gccgatcggg ctctcggtag atccatcagt cggatctgct tcgaggggcc
    11581 cgttacggat ctcacgctaa ccgaaaatac ccaacccgcc ctggtcgcca cgagcagcgc
    11641 gattttggcg gcgttgcggg agcgatatcc gagcttgccg gcaccggtgt tcgccgccgg
    11701 tcattcgctg ggcgagtact cggccctggt agcggcgggt gccctgagcc tgcaggacgc
    11761 ggtgcggctc gtcgagctgc gcggtcgcgc aatgcagagc gcagttccgg aaggcaaggg
    11821 cgccatggtc gccgtcgtag gcggggaccg cgaggccgtg ctcgcgttgt gtgaggaggc
    11881 gaagtcagcg ggcgtggtcg aacccgccaa cttcaacgca cccggtcaag tcgtgctctc
    11941 gggagaagcg gacgccgtgg ccaaggtctc ggcgctcgcc gcggagcgga gtctcaaggc
    12001 gattcctctc aaggtcagcg cacctttcca ctgttcgttg atgaccccag ccgccgaagc
    12061 ggtcgcgcgc gccctggaga ccatcgaggt tcgcccggcg gcgttcccga ttattgccaa
    12121 cgccagcgcc tcccccaacc aggagccgtc ccagacgaag cggctgctgg tcgagcaaat
    12181 tgccggacca gtgcgctggg accaatcaat cagccacatg gcagaagcag gggtaaccca
    12241 cgccctggag attggaccgc aaaaagtgct cgcaaacctg gtcaagcgca tcgacaagcg
    12301 aatccgcgtg ctgagcctct cggatccaga cgcgctcgcc aaggtcagcg aatttctggg
    12361 ctagccggca ctgcctcgtt cgccggaata gggctgggcg gctacgccgc ggcgcgtcgt
    12421 gcagtgcgta acgttctgcc agttgcagag cgcggggcaa agcgtgctaa acacggcgcg
    12481 ctcgtgcctg gcgcacgaga caccctccca tgttcgacct atccgcaaaa gtagcgctcg
    12541 tcactggtgg ctcccgaggt attggccgtt ccgtgggcga gacgctcgcc gcacgaggtg
    12601 cgacggtcgt gctcggttat gcgagcggaa aggatgccgc ggagagcgcg gcggccgcga
    12661 ttcgcgatcg gggtgggaag gcggaagcgc tcgccttgga tgtgactcaa gtggaagcta
    12721 ccgagaaggc gatcgcggag acggcgaagc ggctcgggcg cctcgacatc ttggtcgcca
    12781 acgccggcat cgcgatcgac ggtttgttgc tgcgcctgaa cgaggacgac ttcgaacgca
    12841 cgctcgccgt gaacttgaag ggcgccgttg cctgcgctcg cgccgccgtc aaggtcatga
    12901 tgcgggccaa aaccggccga atcatcttca tctcgagtgt ggtcggagag atgggtaatg
    12961 ccggacagac ggcgtatgcc gcgtccaagg ccgcgctgct gggagtgacc aagtcgatcg
    13021 cgcgcgaata cgcgtcccgc aacatcaccg tcaacgccgt caccccgggt ttcatcgaca
    13081 cggacatgac tggctcgctc acggacgtgc agaaggcgag catcgcctcc gcgatcccgc
    13141 tcggcagaac cggaacgccg ctcgaagttg ctgccgcagt aactttcctg gcctcggatg
    13201 aagccgcgta tgtcaccggt cacgcgctgc gcgtcaacgg tggcatgtac atgtgagcca
    13261 tcaaatttcg accgttactc ataaaaacgc atagcaatgc cactgcctct ttggtagagg
    13321 cgctcgtcgg agaacaagaa catggctgag ggtcgcgaca tcacggccga agtaaagcga
    13381 atcatcaaag agcagctcga cgtcgacgaa aaggacatca aacccgagtc cactttcatc
    13441 gacgacctcg gagctgactc gcttggtctc gtggagctcg tgctcgcatt cgaagaggcg
    13501 ttcgaaatcg acattccgga cgaggacacc gagaagattc gcaccgtgca agacgcggtg
    13561 gattacatcg agaagcacct caacagctga ttttgcgtgt tgccctaccc gaacgcgacg
    13621 gtggtcgctc gcgttgggga gcgcattgaa ttctcaagca ccttcgagtg catcgaatcg
    13681 tgcactaacg agtgcctgcg atttacatcg ctcatgcact ggcggtgctc gagaatcacg
    13741 agagcagatc ggagccagat gagcctcgcg ccgtgaccgc cgcgaggccc tggtcttcta
    13801 tcagggcgta tcaatcggga cacatcacat ggagcgcgtc gtagtcactg gtatcggcct
    13861 cgttacgccg agcggcgttg ggaccgagga gacctggcag tcgctcatcc gcggcaaaag
    13921 cggcgttggg cccatcacgc tatttcccgc gggtcccgag tacgccacac gcatcgcggc
    13981 cgaagtgaag aacttcgatc ccgcggcgtt catggaaaag aagaagttga aggaggtctc
    14041 tcgcttcatt cccttcgcca tgggcgccac caagatggca ttggaagatg ccaatctcga
    14101 gctgacggac gaagagcgtg agcgcgcggg tgtgttcatt ggcgtcgggc tcggcggcct
    14161 cgagaacctc gaacgatgca cgctgacgct ggcgcaaaag gggccgggta aggttagccc
    14221 ttacttcatt ccctctttga tcgcgaacat ggccgcgggt caggtctcga tcgcgttcgg
    14281 cttccgcggc ccgagtttgg cgcataccag cgcttgctcg tcgagcgctc acgcgctggg
    14341 tgaggcgctg cgctggatgc agcgcggtga cgccgaggtc atgattgcgg gtggcgcgga
    14401 agcgaccatc acgccgatcg gcatcggcgg gttcagcgcg atgttcgcct tgagccgtcg
    14461 caatgacgaa cccgaacgag ccagccgtcc ctgggatcgc gggcgagacg gcttcgtgtg
    14521 cggcgaaggc gcgggcacgc tggtgctgga aactttgacg cgggccaagc ggcgcggcgc
    14581 ccggatttac gcggagctcg ttggctacgg cgccacgagc gacgcgcatc acatcacgaa
    14641 gcccgccgcc aacggcgcgg gtggcgcgcg cgcgatgcgg atggccctca aggacgcgaa
    14701 gctcgcgccg gaagccatcg actacatcaa cgcgcacggc acctccacgc ccgccggaga
    14761 cctggaagaa gccaaggcca tcgcagacgt gttcggtgcg cacgccacgg acaagaagct
    14821 gtgggtcagc tctaccaagt ccatgatggg gcacctgctc ggcgcggcgg gtgccgtcga
    14881 agccgcggtc tgcatcaagg ccatgggctc gagcctggtg ccgcccacta tcaacctcga
    14941 ggatccagac ccggaagctg gcctggattt cgtgccgaat cagacccgtg agcgccgcat
    15001 tcgccacgcc atgtcgaact cgttcggctt tggcggcacc aacgcgaccg tggttttctc
    15061 ggccttcgaa ggctaacggt ccgcactcat gcagtgccct ttctgccgcg agatggacag
    15121 tcgcgtcgtg gactcgcggc tcgtggccgg gggcactgcg atctggcggc gccgcgcgtg
    15181 cgatgcatgc aaccggcgct tcacgaccta cgagcgggtc gagtaccagc tgcccatggt
    15241 cgtgaagaag gatggtcagc gtgagccctt ctcacgggac aagctgctca gcagcctacg
    15301 gatcgcctgc aacaagcgtc cggtagccac ggacgcgctc gaggaacacg cagctcagct
    15361 ggaacgtgag ttggccgaga gcggcgagaa agaaatctcg acgcgcgtca tcggcgagcg
    15421 agtcatggag cgcctgaaac agctcgatgg cgtcgcctat gtgcgttttg cgagcgtgta
    15481 ccgctctttc cgggacatcg acgagttcat ggccgagatg ggcaaacttg tgcgctcacg
    15541 aggtgcgggc gatgctcccg agtgacgccg gactgaacgg ttcccgacgg tcctgctgga
    15601 ctcttgccgc gagcgcggca aattgcgcag tgtgaccccg gcggcagcga caccccatgg
    15661 ccgatctcga tactaagctg atggctcgcg ccctggagct cgggcgcgcg ggtgatcctt
    15721 ctccgaaccc gcacgtcggg tgtgtcatcg cggacaatga gcgcatcgtc gcggagggct
    15781 accacaccgc cgcgggccac gaccacgcgg agatcgtggc gctgaagaag gcgggctcga
    15841 acgcccgcgg ctgcacgctt tacgtcaccc tggagccctg caaccacgcc ggtcgcacgc
    15901 cgccgtgcgt tgacgccatc gccgccgccg gcatccgtcg agtcgtgatt gggaccaggg
    15961 atccgaatcc acgcgtcgtg ggtggcggga tcgagcggct gcgcacgctc ggcatcgaag
    16021 tgacggaagg tgtgctggag agcgaggctc tcgagctcat caagccctgg accaaattcg
    16081 tgaaccagga ctcgagctac ttagcgttga agctcgcgct gtccctggat ggtcgcattg
    16141 ccacccgcag tggggcgtcc aaatggatca cgtgccccga gtcgaggctg cgggtgcaga
    16201 cgctccgcgc gcaccatgac gcagtcatgg ttggtgtgaa cacggtgcta acggacaatc
    16261 cgcggctcac cgtgcgcgac gtgccggggc gaagtccggt gcgcgtcgtg gtggatagtc
    16321 gtctgcgtat cccgctggat tgtcaggtcg tcgctaccgc gcgtgagatc cccacctgcg
    16381 ttgccaccac cccacaagcc ccgcgggcca gca
//
LOCUS       DASTKU010000940         3632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1076673, whole genome shotgun sequence.
ACCESSION   DASTKU010000940 DASTKU010000000
VERSION     DASTKU010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076673"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            526..1221
                     /locus_tag="VFQ61_33250"
     CDS             526..1221
                     /locus_tag="VFQ61_33250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959421.1"
                     /translation="MEDDSNSFGTPKPLTPPPSTSPLRRGLSSSPLLFGSAFALAVAA
                     CGSADDDDPVPPMVPPMPMGGSSGAAGAAQGGSSVIAPMVPPQGGSGGTTGSSAAGSG
                     GFVPPMPPPMTAPEGGATNTNSGGSGGVIPPMVPRGGSSGAPMIAPSGGYTVTPMVPP
                     MVPRGGSAGAPMFPPPNWGGSAGAPMIAPSGGRSFGGVPPMPGPQGGTSENSTGGTGP
                     IPPMPPPRGGNEA"
     gene            1326..2288
                     /locus_tag="VFQ61_33255"
     CDS             1326..2288
                     /locus_tag="VFQ61_33255"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HET9959422.1"
                     /translation="MAENLLLGAETSTLVRALVSRGVPASLAERRVAELARSPILRHA
                     APHLIQERRADLLIRLQRTVARQAQHPASIERRPTPSASDFYDHYCAWQVPVIFTDLL
                     ATTAARHWTPESLAERFGGVELKVASGRNSDPDYDRNTPKHTSSMKLGEYVDRVLASG
                     ATNDIYMVANHRNIARPELRPLLDELTFPEGYLDPSQLTDASALWFGPAGTVTPLHHD
                     TSNILFCQIYGRKRFRLVPQFELPLHDRARSLYSSADPESESDAQFFSELVVKDVVLE
                     PGETLFIPACFWHHVRSLDVSISFATTALARPNNFDWFRPGEVS"
     gene            2358..>3632
                     /locus_tag="VFQ61_33260"
     CDS             2358..>3632
                     /locus_tag="VFQ61_33260"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein"
                     /protein_id="HET9959423.1"
                     /translation="MAVATAVPALDLLHPTPSWRIDFEGTRVAIHLAFAGGVSGGLFS
                     EALEHATFAPSTWDSNTFANDLFLQRFVTHCLRIRIGRQEPLANTTHLTRLLAQPPVD
                     PSIVRYRRAILAELVEVPELRPQLERLYLDICRLRALLENSGNLKNWDPNRRQLDVLG
                     VLKEIIDGLSTGFRTARSGLHLLNYFGESVRRSEPYQALSDLLSFDGQLATLSLNVEV
                     GADGRLRGFQIQALRENQENPFVNPAWRRWLAKLELFVRGYKFGDGEIMARLVDAVFS
                     GLEDVTVGLVQLLGDLEFYLAALSFRDHAASAGLAVCLPELVEAHEPRVLLGLFNPFL
                     LAQGSAVPCNLVSDSFTTTTLVTGPNSGGKTRLLQAIGLAQVLAQAGLFVPARSGSIA
                     LTQGLVVSLIEETKSDQTEGRLGMELMRIRTLF"
BASE COUNT          639 a         1174 c         1115 g          704 t
ORIGIN      
        1 gactcgaata caggtgtgtc attgacgctg gcccccgatt caaggcgagc catcgaatct
       61 agggcgcgac ccaggctggc gatggcagcc cgagattttc atcaacggtc aatccccgat
      121 tgtctcgtcg ttcgcaagat gttgcgtggg tgaacggccg cgccgaaccc gcaatttcgt
      181 gagggatcag agggatcacc aatgttagag tagattctat ccgcggccct cctggattct
      241 agggggccat gagcatctag ccgattagtc gctttccacc ccgttcggcc gtcccttgcc
      301 gtctcgctca ggcaaaaaca gacttctcac gctaccccgc cgccgtggca cagtctgggc
      361 cagcgcgtgg ctgcgccagt tccgctggcc cagactccga actggccgag gaggagcgca
      421 ttctcgagct gcccggggaa gttcgctgct aaacgacgac cgcaggaggc aaagaaatca
      481 tcgacccgtg caacaggcac ggtgtttgct gcaagatgcc ccacgatgga agacgactcg
      541 aattcattcg gcacgcccaa gccactcacg ccgccgccca gcacttcacc gctacggcgc
      601 ggcttgagtt cgagcccgct gctgttcggg agcgcattcg cgctggcggt ggcggcctgt
      661 ggaagcgcag acgatgacga tcccgtgcca cccatggtgc cgcccatgcc catgggaggt
      721 tcgagcggcg cagcgggcgc agcacaaggc ggctcgagcg tcattgctcc catggttcct
      781 cctcaaggcg gctcgggcgg tacgactggt tcgagcgccg caggctcagg aggcttcgtc
      841 ccaccgatgc cgcctccgat gacagcgccc gaaggcggcg cgacgaatac caactcgggc
      901 ggcagcggcg gagtaatccc accgatggtg ccgcgcggcg gtagctcggg agctccgatg
      961 atcgcacctt cgggcgggta tacggtgact ccgatggtcc cgccgatggt gccgcgcggc
     1021 ggtagcgcgg gagctccgat gttccccccg cccaattggg gaggcagcgc aggcgctccg
     1081 atgattgctc cttcgggcgg aagatctttc gggggcgtgc cgccgatgcc cggtccgcaa
     1141 ggcggcacga gcgaaaactc gaccggaggc accggtccga ttccgcccat gcccccgccc
     1201 cgcggcggca atgaagcctg agatcacgcg gttaccgtat gccgatcacc tatcgcaggc
     1261 aggggcgcgc acggagtctt cgtagccatc cgcaatctga cctaagccct gagtggttga
     1321 gttgggtggc ggagaattta ctgctggggg ccgagacctc tacgctcgtg cgagcgctgg
     1381 tctcgcgcgg cgtgcctgcg tcgttggctg aacgtcgggt ggccgaactc gcgcgctcac
     1441 ccattttgcg gcacgccgct ccgcatttga tccaggagcg acgcgcggat ttgttgattc
     1501 gtttgcagcg caccgtagct cgacaagccc agcatccggc gagcatcgag cggcgcccca
     1561 cgcccagcgc gagcgacttt tacgaccact actgtgcttg gcaggtgccc gtcatcttca
     1621 cggacctgct ggcaactacc gccgcgcgcc actggacccc ggagtctctc gctgaacgtt
     1681 ttggaggtgt ggaattgaag gtggcctccg ggcgcaactc ggatccggac tacgaccgca
     1741 acacgcctaa acacacgagc tcgatgaagc ttggtgaata cgtggatcgc gtgctcgcga
     1801 gcggggccac gaatgacatc tatatggtgg cgaaccaccg caacatcgcg cgcccggagc
     1861 tcaggccgtt gctcgatgag ctcaccttcc ccgaaggcta cctcgacccg tcccagctga
     1921 ccgacgcgag cgcgctctgg ttcggaccgg cgggcaccgt caccccgctg caccacgaca
     1981 cttcaaacat cctgttttgc cagatttacg gccgcaagcg cttccggctc gttccacagt
     2041 tcgagctccc gctgcacgat cgagcccgta gcctctactc ctcggcagat ccagagtctg
     2101 agtccgatgc ccagttcttc tctgagctcg tggttaaaga cgtagttctg gagccggggg
     2161 aaacactctt tattccggcg tgcttttggc accatgtgcg ctcactcgat gtaagcatca
     2221 gttttgccac gaccgccttg gctcgcccca acaacttcga ttggtttcgg cccggcgaag
     2281 tctcgtagcg cccgttctat ttccaaaaga acgtcagccg cgggaacacc ggcgacgcgg
     2341 cgctgtcggt tgcgcgcatg gccgtagcca ccgccgtacc cgccctcgat ctgctgcatc
     2401 ccacgccgag ttggcgcatc gatttcgagg gaacccgagt cgcgattcat ttggcatttg
     2461 cgggcggcgt atcgggcggc ctgttcagtg aagcgttgga gcacgcgacc ttcgcgccct
     2521 cgacatggga ttcgaatacg tttgcgaatg acctcttcct gcagcgcttc gtcacccact
     2581 gtttgcgaat ccgaatcggc cgacaagagc cgctcgccaa caccacgcac ctgacgcgcc
     2641 tgctcgccca gccccccgtg gatccgagca tcgtgcggta tcgtcgcgcg attctggcgg
     2701 agttagtgga ggttccggag ttacggccgc agctcgagcg cttgtatctc gacatctgtc
     2761 gattgcgcgc gttgctcgag aactcgggta acttgaagaa ttgggacccg aatcgccgac
     2821 aactcgacgt gctgggggtg ctcaaggaaa tcatcgacgg actgagcacg ggttttcgaa
     2881 ccgcccgctc cgggctgcat ttactgaatt acttcggaga gagcgtgcgg cgcagtgagc
     2941 cgtaccaagc cctctccgat ttgctctcct tcgacggcca gctcgccacc ctcagcctca
     3001 atgtagaggt gggcgcggat gggcggctgc gcggatttca gatccaggca ttgcgagaga
     3061 atcaagagaa ccccttcgtg aacccggcct ggcggcgctg gctcgccaag ctcgagttgt
     3121 tcgtacgcgg ttacaaattc ggtgacggcg agatcatggc tcggctcgtc gatgccgtgt
     3181 tctcgggact cgaagatgta acggtgggtc tggtccaatt gctaggggat ctggagttct
     3241 atttggccgc gctgagcttc agagatcacg cggcaagcgc gggcctggcc gtgtgtttgc
     3301 ccgaactggt cgaagcccac gagccccgtg ttttgctggg tcttttcaat ccgtttctgc
     3361 tcgcgcaggg ctctgccgtc ccctgcaact tggtgagcga ctcgttcacc acgacgacgc
     3421 tcgtcacggg ccccaactcc ggcgggaaaa cccgtctgct acaagccatt gggctagccc
     3481 aagtgctggc ccaggcgggc ctgttcgtgc cggcgcgctc gggttctatt gccctgaccc
     3541 aagggctcgt cgtctccctg attgaagaaa ccaaatccga tcaaaccgag ggacggctcg
     3601 gcatggagct gatgcgcatc cgcaccctct tc
//
LOCUS       DASTKU010000941         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1747205, whole genome shotgun sequence.
ACCESSION   DASTKU010000941 DASTKU010000000
VERSION     DASTKU010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747205"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFQ61_33265"
     CDS             complement(<1..328)
                     /locus_tag="VFQ61_33265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9959424.1"
                     /translation="MAHSPEQTSCDEGALILKVAEGDVASYRELVKRHAARLVRFATR
                     LLRDASEAEDVVQESFLRLWLRASEYSDKASGSAWLYRIAHNLAVDRLRKRGKLEALE
                     EQELAPV"
     gene            complement(377..814)
                     /locus_tag="VFQ61_33270"
     CDS             complement(377..814)
                     /locus_tag="VFQ61_33270"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959425.1"
                     /translation="MKKSIFLSVFAAATTLSALVFACPGHGPEGRAKLDTNSDGKITL
                     DEMRTAAKERFGRIDADKNGVLSKEELQKRGRARMMLERADTNGDGQITTAELEAHTA
                     TKFKEMDTNKDGALSHEELRAGFKHGFKHRHGGPDGKQPPAKS"
     gene            995..2440
                     /locus_tag="VFQ61_33275"
     CDS             995..2440
                     /locus_tag="VFQ61_33275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959426.1"
                     /translation="MMNSPHERAEGQLGECEAIFELFEGPLGRTRLGRLYGSGHGGRL
                     VTLRPLRSRVPAQLLSRARQVAHPRLTKVLGLSTIDGAPHLVSEYIEGASVFELLRAA
                     REKGSRVDPRVAVRVVLDALKASSAAKELLQSGEDPAQGAVLADALWIAHFGETLLMD
                     AGVSACVCGSNDPLINAQALGGDVVFQASVLLDAFLSNRATEAVTHDRDVGALPDVGS
                     LPPELGQLLLRFAKPEPGASAQAFIADLEALPRSWIAQEMELGQALTRHLGPVLELRR
                     HKLLMLERDAGGSSEDESEVTRFFTPLNLEKEGQHDTVRPAAPDAVPAGPPESPSKAL
                     TETANARSSEEPSESAEGEPEIERAAAEGASSDRPAPRPSFPSVPQSSSEQPLEPRSV
                     ESGPPAAVESEEDTPALRRSRRLAFALISAALVVLTLAILRATPVWQSGSAGREKTRR
                     ESRALPSQPSPAPSAPTRGDAPASNESPVKP"
BASE COUNT          455 a          813 c          844 g          550 t
ORIGIN      
        1 aaacgggcgc gagctcttgc tcctctaaag cttcgagctt gccccgtttt ctcaagcgat
       61 cgacggcaag gttgtgagcg attcggtaga gccaagcgct gccgctggcc ttgtccgagt
      121 actcactcgc gcgcagccaa agcctaagga agctctcctg tacgacgtcc tcggcttcgc
      181 tcgcatcgcg cagcaagcgg gtcgcgaagc gaacgagccg ggcggcgtgg cgcttcacta
      241 actctcggta ggacgccacg tcgccttctg cgactttcag gatgagcgca ccctcgtcgc
      301 acgatgtctg ctcgggtgag tgcgccacgg tcctttatct actctatcag ccggtgaacg
      361 tcggacgcgt caggtttcaa gatttagcag gcggttgctt gccgtccggg ccgccatgac
      421 ggtgcttgaa gccgtgtttg aagccagcac gcagctcttc gtggctcagt gccccatcct
      481 tgttcgtgtc catttccttg aattttgtgg cggtatgggc ttctagttct gcagtggtga
      541 tttggccatc accgttggta tccgcgcgtt ccagcatcat gcgggctcgg ccgcgtttct
      601 gaagctcttc cttgctcaac acgccattct tgtcggcgtc gatgcggcca aaacgctctt
      661 tggcagccgt tcgcatctcg tccaaagtaa tcttgccgtc gctgttcgtg tcgagtttgg
      721 cccgaccttc cggcccgtgt ccggggcaag cgaacacgag agcactcaaa gtcgtggcgg
      781 ccgcgaacac actcagaaag atcgactttt tcatgattcc gttcggtatc ctttcgcccg
      841 ctctgcgcag gcctcacaga gctcaacgca agagggtccg aggatccgtc gcggccttcg
      901 aatttttttt gaggccagag cgtgcagtaa gactggatgc ccgcgctcgc ttaacgcaat
      961 atgagttccg gtcgttcggc gctgagaggc atacatgatg aactcgccac acgagagagc
     1021 ggaaggtcag cttggcgagt gtgaggcgat cttcgaactg ttcgagggtc cgctgggccg
     1081 cacgagacta ggtcggctct atggcagcgg tcacggtggg cgtctggtta cattgcgacc
     1141 tttgcgtagt cgcgttccgg cacagttgct gagccgtgcg cggcaggttg ctcatccccg
     1201 gctcaccaaa gttctgggcc taagcacgat cgatggcgcg ccccacttgg tcagcgaata
     1261 catcgagggc gcgtccgtct tcgagttgtt gcgtgccgct cgtgaaaaag gcagtcgggt
     1321 cgacccgcgc gttgcggtgc gtgtcgtatt ggatgccttg aaggcgagca gcgctgccaa
     1381 agagcttctt cagagcggtg aggatcccgc tcaaggggcg gtgttggccg atgctctgtg
     1441 gatcgcgcat ttcggtgaga cgctgctgat ggatgcaggc gtttccgctt gtgtatgcgg
     1501 ttcgaacgat ccgctcatca acgcccaagc gctcggcgga gacgtggtgt ttcaagcgag
     1561 cgtgttgctc gacgcattcc tgtcgaatcg ggccacggaa gcggtcacgc atgaccggga
     1621 tgtcggcgct ctgccggacg tcggttcgct acctcccgag ctgggtcagc tgttgctgcg
     1681 ctttgcgaaa cctgaaccag gcgcctccgc gcaggcgttc atcgctgatt tggaggctct
     1741 acccaggtct tggatcgcgc aggaaatgga gctcggccaa gctttgacca ggcatctcgg
     1801 ccccgtgctc gagctgcggc gccacaagct cttgatgctc gaacgggatg cgggagggag
     1861 cagcgaagac gagtccgaag ttacgcgctt cttcacgccg ttgaacctcg agaaggaagg
     1921 gcaacacgac accgttcgtc ccgcggctcc agacgcggtg cccgcaggac cacccgagtc
     1981 gccatcgaaa gcgctcaccg aaacggcgaa tgctagatcg tcggaagagc cgagcgagtc
     2041 cgccgagggc gagcccgaga tcgagcgcgc agccgccgag ggcgcgagta gcgatagacc
     2101 cgcgcctcgc ccatcttttc cgtccgtgcc gcagtcgagt tcagaacagc cactcgagcc
     2161 tcgctcggtc gagtctggcc cgcccgcggc agtggaatcg gaggaggata ctccggctct
     2221 gcgacgctcg cgccgcctcg cgttcgctct gatcagcgcg gcactcgtgg tgttgacgct
     2281 cgcgattctg agagccactc cggtttggca gtcgggcagt gcgggccgcg agaagactcg
     2341 acgggaatcg agggccttgc cgtcgcagcc ttcgccggca ccgtcggcgc ctacccgtgg
     2401 cgatgctccg gccagcaacg aatctcccgt aaagccttga cccgcttgcg agctagaggg
     2461 tctgcccagg ctctgctgcg cgtcctgagc atgttctggg gaacgaccgc cgccgcgtcg
     2521 ggctagactc tatcgaatgt cggagaacga atcgccgatc gatcgcctgg tggcggacgt
     2581 gcgggccaag ctgcatgctc gacgaacggc cctgctcgat cgcgtaggcc gcatcgaagc
     2641 cgccctcgag aattatcggg tt
//
LOCUS       DASTKU010000942        21670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1077260, whole genome shotgun sequence.
ACCESSION   DASTKU010000942 DASTKU010000000
VERSION     DASTKU010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 21670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21670
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077260"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1041)
                     /locus_tag="VFQ61_33280"
     CDS             complement(154..1041)
                     /locus_tag="VFQ61_33280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959427.1"
                     /translation="MTNRNCGKVGVATRWGALARYAAALGMVSAVSACSAAPEAGEPN
                     DAISETKQEIYSTYTWTQNGATQQMLPVSEGFCFITKITGKFRGDQEWVSLDHSSGFW
                     TLSGHSNQHSVAVQAACVKYSELGASSGSVPYDTGWVSGYTWVLTDPNEFCYMTGFKG
                     QADSYRDGVWVYPLPGSVPGYEIEAYHSGGNGIWGKMSCVRLADKNFLPFGSYQLSQK
                     WTDFCTNNTSCYNSQTISSSQRFYCMLSQVQGQLRGSGEIASVVRNSDTNWTLNADSM
                     RACSDGNCQLTTTATCNFY"
     gene            1640..2359
                     /locus_tag="VFQ61_33285"
     CDS             1640..2359
                     /locus_tag="VFQ61_33285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959428.1"
                     /translation="MGRNLFCGLGLLLLCISCGDTEESPAPAHGGANTSGTGGMGGDG
                     GSSGEGSIRGGSSNGGSTESGGQAGADGGSREPATHSGGAPESGGSGGVAGATGSEPQ
                     AGEPGVAYCDRGFGVYNDKGVGAGCQDPGLAESLACPAKFCLTRVLENRWQKEGDFST
                     PSHCSIQCDRDTDCGDGFQCCQATNQAFCLDNPTGNFAILGCAEVCPDNFLGCGEGQT
                     CCESLGKICVSNDCHGVCLGQ"
     gene            2470..3015
                     /locus_tag="VFQ61_33290"
     CDS             2470..3015
                     /locus_tag="VFQ61_33290"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HET9959429.1"
                     /translation="MVRNHGAFYLAAMVFQIVRAVRRLNEIGLEVRTKRLLVFIAGVV
                     AVTSAFLPRAGRADSARGTLVLRVSGCKHDRGKVVARIFRRSDNAPRGPGYRSTAGKI
                     RTGKSVLEFSDLPHDTYAVFVFHDENANGIPDHNLFGLPSEPLGFSRGYRATLFSGIP
                     SFRDLSFRFDSNTKPLVLQVD"
     gene            complement(3193..3456)
                     /locus_tag="VFQ61_33295"
     CDS             complement(3193..3456)
                     /locus_tag="VFQ61_33295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959430.1"
                     /translation="MARVVRTQRSLASALLALFPRTCARVYVSFRGGDEHATTPRGGS
                     VHSVLQAQLSLDHVVHEIVWDIVIVPGRRIQRARGLPRQRYDH"
     gene            complement(4378..5019)
                     /gene="maiA"
                     /locus_tag="VFQ61_33300"
     CDS             complement(4378..5019)
                     /gene="maiA"
                     /locus_tag="VFQ61_33300"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HET9959431.1"
                     /translation="MKLHNYFRSSASYRVRIALNLKGLSYEYVSVHLSRGLGEQFAAE
                     FRALNSASLVPVLQDGDRRITQSLAIIEYLDEIHPEPPLLPSNPFGRARVRSLALTVA
                     CEIHPLNNLRVLNYMTQQLGLDSDARTRWYQHWVELGLSTLEAELAIGPPGRFCHGDS
                     PGLADCCLIPQLFNARRFKCDVSRYPRLLAVEQSCASLPAFRDAAPERQPDAE"
     gene            complement(5016..5726)
                     /locus_tag="VFQ61_33305"
     CDS             complement(5016..5726)
                     /locus_tag="VFQ61_33305"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET9959432.1"
                     /translation="MNYIFPAPPVSTVPIAGADASFPVHRIYCVGRNYAEHAREMGFE
                     PEREPPFFFTKPADAIVANGATIPYALATQNLHHEIELVVAIGRGGARISRETALEHA
                     YGYAVGIDMTRRDLQLAARDKGRPWDVGKAFDDSAPISAIRRATDIGHPKKGNIRLSV
                     NGVDRQNADLEQLLWPVADLIVELSKLFVLRPGDLIYTGTPAGVGPVKPGDELRGNIE
                     GVGELAIRIGPAHAEATP"
     gene            complement(5755..6258)
                     /locus_tag="VFQ61_33310"
     CDS             complement(5755..6258)
                     /locus_tag="VFQ61_33310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959433.1"
                     /translation="MSDWLFMLGEPLGQREREQVREYLTGLGLEQELSFETVKDFAAA
                     ARIIAHPDWDRRWWDAEQRERERLRNQLVSTPGSGQRLRALSDFVQDSISEHHGTAAA
                     QATRLGCADAGLIRAAAGALGEALYLGKLAQLAGAPASHPFLLKQALFEGGHWPLIVL
                     AGHFYVF"
     gene            complement(6255..7310)
                     /gene="gtdA"
                     /locus_tag="VFQ61_33315"
     CDS             complement(6255..7310)
                     /gene="gtdA"
                     /locus_tag="VFQ61_33315"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HET9959434.1"
                     /translation="MRAALPTSSAQASDARAEFYRRIDRDHLTPLWESLAQLVPPQPS
                     SPCVPALWRYEAVRPHLMEAGRLISAQEAERRVLVMENPGLRGASSITRTLYAGLQLI
                     LPGERAPSHRHTQSALRFVVEGTGAYTAVNGEPAFMQPGDFIITPSWAYHDHANPGDN
                     PVVWLDGLDIPMISFFDAGFAERSAEPKQALTRPDGDALSRYGAGLLPYEYQSPSLST
                     PLLSYPYARSRAALRALHRQAELSSFHGVKMQYSNPVTGGYPMPTIAAYLQLLPEGFR
                     GRGYRSTDGTVYCVVEGHGRSRIGDQLFDWAPKDVFVVPSWCPVQHEADADSVLFSYS
                     DRPVQKALGIWREDAIP"
     gene            complement(7315..8025)
                     /locus_tag="VFQ61_33320"
     CDS             complement(7315..8025)
                     /locus_tag="VFQ61_33320"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HET9959435.1"
                     /translation="MTTAYHEVKTVADLASEPAEYREAISKIVVSHTVNELYGAQVFD
                     EPAIALAPTPYAKWLSCRIAMEEYHHHVRFKGLADELGVPPSATDPNVKRPLTIFGFA
                     MKTWPEFCVIKMLADLAEILQVEDLLHCSFVPLRNIGRVTMPEEKFHAQFGKDFCTEL
                     CKTEEGRRAVQQAIDDYAPFLPQFFGAAGSKNNELYRKYGLKERTNEQMREDFLARAR
                     GVVESLGLRFPALDIPQG"
     gene            complement(8117..9058)
                     /locus_tag="VFQ61_33325"
     CDS             complement(8117..9058)
                     /locus_tag="VFQ61_33325"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9959436.1"
                     /translation="MVNTPASNSLNERVALVTGGARGIGLAISRALHARGASVVIADS
                     GVSISGTAPDPSIADAAAQELGARAAAFSSDISSPEGAAEAVALAMDRFGAIDLVVNN
                     AAILRDSLIFKANPANWNAVLQSNLSAAFYVLAAATPHLREQAKSGRAPGRVVNITSS
                     TGLYGNFGQSAYASAKAGLLGLTRVTAMDMARSGVTCNAVAPFGATRVTEAIQPANPA
                     QAQYKERALRVSARFVGDFVAFLCGPEAQTLSGQLFGVRGREVFLFSQPRPLAHFVMP
                     ETDDGAATLPGVVREHFTNRLVELTTDLEAFNTEPVS"
     gene            complement(9077..10105)
                     /locus_tag="VFQ61_33330"
     CDS             complement(9077..10105)
                     /locus_tag="VFQ61_33330"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9959437.1"
                     /translation="MSSRIRVLSNPPQSAPAPAGETILESLLRAGVPFPHNCQSGNCG
                     ACKCELVEGDVLDLPYSEYALSAEERARNLILACRTQVWGDCTVRPLEADEMVLHPSR
                     ILRCRVARIRELTHDIRGLELEIESGGPYGFSAGQHARLKFAPGIPERSYSMANCPDH
                     PSLAFHVRRVPRGQASGYAFTSLRPGAEVVVAGPFGNAYLRANHRGPILAVAGGSGMA
                     PIQSIVETALGSDPERVIHLYFGVRDERDVYLEEHLAALVRRHPNLHVQVILSQPSAP
                     TSRRTGTVAAAVSEDFGELSGYKAYVAGPPPMVESVQHELLGKGVATRDVHADAFYSQ
                     SDDAFNTW"
     gene            complement(10102..11157)
                     /locus_tag="VFQ61_33335"
     CDS             complement(10102..11157)
                     /locus_tag="VFQ61_33335"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9959438.1"
                     /translation="MTSSVARSRTIAVLAAGLWVIASLLDESTLGVYVLLMLTACVTV
                     GLTLLMGHAGQVSLGHGAFYACGAYAAGLLATHGYPTWVGLVFAPMAAAAIATVVGVP
                     LLLLRGHHLAFATLALHLIFLSSISELSITGGDVGLQGIPPLAVVGFTLTSVRGYAYL
                     GSLALVLVVVISSNVLASRPGRALRALSSSEVAAASSGVAVGQYKLAVFALSAGFAGL
                     AGGIYAFYMGYLAPGSFPVLLSIEYIVMAEVGGLGRISGALVGSALVYLLVHGLSRIA
                     TLSGMPSTAPVILSYAVYAALLIGAVLFLPGGVVSLVSRTWVGARSRWAAHARSSAPV
                     RHSTAEKAIPSEHGSHS"
     gene            complement(11154..12014)
                     /locus_tag="VFQ61_33340"
     CDS             complement(11154..12014)
                     /locus_tag="VFQ61_33340"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET9959439.1"
                     /translation="MSAFLSYLVHGICVGCGFALVASGIIIVYRVTRVVNLAQGMFSV
                     VAGFAATTLLGVGLPHGVSELLAIGAAALVGLVTGCVAIGKRGISPQASLIATLGVGV
                     FSYAVEVLVWGDQPRSFPGLAGTQGFSNLEVPNQYLLITVVTLLVFTALELFFERTYA
                     GKALSACASNPYAAALQGIGVQRMGLYGFTLGGALGGIAGVLLTPLQPISYDSDVSLF
                     VNGFASAILAGLKRPVLGLAGGLVLGVAESMVAGYAKASYQSSLALLLTLAILVAQAT
                     RRESLHAVEE"
     gene            complement(12011..12679)
                     /locus_tag="VFQ61_33345"
     CDS             complement(12011..12679)
                     /locus_tag="VFQ61_33345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9959440.1"
                     /translation="MIRVEALTVRYGAATALDRVSLTVSAGEKVALIGANGAGKSTLL
                     NALSGVIPKAAGSLRVEGRWAHVPEGRQLFPDLDVEDNLRLGAFRVRERDPAWIYELL
                     PELARVRRQRAGSLSGGQQQMVAIGRGLMARPDVLAIDEMSLGLAPLIVEQLADRLAT
                     LNATRGLTLLLVEQSARVALSLCDRAYVLETGRVVAQGSAKELADSPFVESAYLGSPL
                     ERPS"
     gene            complement(12676..13440)
                     /locus_tag="VFQ61_33350"
     CDS             complement(12676..13440)
                     /locus_tag="VFQ61_33350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9959441.1"
                     /translation="MMLHVEGLSRRFGGVYAVRDVSFRVQPGELRGVIGPNGAGKSTL
                     FHLIAGHLKPSAGRVSLGTEALDGYRPDQRARLGVAIVYQGDRIFRRMTVRENVMVGA
                     HCRSRHGFLAAALRLPSCRREEKELRARADALLDQVGLSAVAEQRAESLPLGQQRTVQ
                     LARALAADPKLLLLDEPAAGLHAAERTQFARLIREVRGRGLTVLLVEHDVALVTSLSD
                     RITVLDLGRVIAEGEPSAIRRDPAVLAAYLGGTPAS"
     gene            complement(13440..14675)
                     /locus_tag="VFQ61_33355"
     CDS             complement(13440..14675)
                     /locus_tag="VFQ61_33355"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9959442.1"
                     /translation="MVCTRRALASLALVLMVSACRKSTEPAPVKELHREANAAATPKS
                     ETIRVGLIASLTGKYSALGSEDKKAVELAIEQINSSGGLLGKRLEVISRDDQTLPDKS
                     VLAYNELKAENVVAVIGSVFSNSALATLPMAQRDGIPYLSLTPAEEQVIPIKSYVFVV
                     PALANAYAERYLEYMQATKITKIAVAHDTKGAYAISGYNTTKSLASKYGVQIVRDEVF
                     ETTTNDFSPLFTHLKGSGAQAFVFWGTGPSGVTATKQYAAAGLKMPLFMTPAQASKLW
                     IDPVGTAGEGVTVLSAIGVVGDYLADGPQKQVIQRMSVPFQQRHGYPPPQFAQDGYSA
                     CLLLFEAIKKAGSAERPRIQQALENLSLLTPNGHYRYSPTDHSGLTRDYISVNVVREG
                     KLVPTEWAQEKLSQTLAQQ"
     gene            complement(14728..16149)
                     /locus_tag="VFQ61_33360"
     CDS             complement(14728..16149)
                     /locus_tag="VFQ61_33360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959443.1"
                     /translation="MSGRSTPVRHHWLLRACLHLGPFLWVSSATAQQATPPQDGAPVA
                     EPGAEHPQDTPAPSADSPPPAASPSPGPTAPPSVAAPAAPSTAPAPPPAGTPPAGPPA
                     SSIPDGEAPAGPIAPAFPPAIPNIDYGARMQAGLKFQNPDHPSRMDDISEVLTADLYM
                     SGQIHRYLKWQVSVTMSYPGTVGSPGTVNVTPHDVIARIEPIPEFNVYLGRMLVVVDR
                     FAPSGPWGMDEFFLPGFFPLVAPPALPKAGASGRDVGVTVWGAPLKGHVKYYLGAFNL
                     YDPALNPLLSGRLQVSLLNGEPAFYHRTTYFGTKDLVSFGVGGQYQKKGSVQTVPVTT
                     PPTPPLTDDYDNVTADLTVEKNIADAGTVSLVGAYYKYGGDFQRWKDFWVASAGYMLP
                     KPLGIGKVRATVRYQRALDNSPGADPTSLVEAQLSYNVAAWFARFQLGYRHGDTYLAP
                     TATTPARSQASNMAYFGVTLADP"
     gene            16589..19759
                     /locus_tag="VFQ61_33365"
     CDS             16589..19759
                     /locus_tag="VFQ61_33365"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET9959444.1"
                     /translation="MTRFESLSNDLFALPAVELVRRERDQLLRSRHALRPYARCSGEF
                     LEYWARCAPEREFVLERNETGWRALSYGEMLDRVRRVGAWLLGTGLSAQRPVAILSEN
                     SLEQAVLTLACLHVGVPVAPISPAYSLVSKDFGKLRSILRTLRPGLVYVSDLARFAPA
                     LSKVRDVHDGSIVSGERYPGDGVLAFDTLLRARDDAAVERAFEAITADTIAKLMFTSG
                     STSEPKAVINTQRMLCSNQEAIAQIWPFVNRPPVLLDWLPWHHTFGGNHNFNIVLRNG
                     GTLYIDGGRPLPGEFEQTLSNLREVAPTLSLNVPRAYDLLVSALQRDSELRSRFFSRL
                     ELIFYAGAALPQHLWEALRSLARQTLGHDLPLVSSWGLTETAPTATIGHFSPDRAGII
                     GLPVPGSELKLVPNGDKTEVRVRGPNVTPGYFRREELTANSFDDEGFFRTGDAVRFVD
                     PKHPERGLVFDGRIGEDFKLSTATWVNVGALRLEAISALAPVAQDVVVTGHDRDEIGL
                     LIFPSIAGCRSLCSDLAAEAAPGALFEHPAVRARVAAGLSALARASTGSSTFPRRALV
                     LAEPACADAGEITDKGYINQRAVLTRRAALVDVLYEQPLQAGVITVSPDEVASSGRAQ
                     VFRGRSAAPPLESNMYEASDPFALVARYDDIWLLGGVRTPFADYTSILSGVSATDLGI
                     HVARALFQRSDVPARDVGAVIAGNVAQSSFDAFYLPRHIGLYSGVPLGVPALLVHRLC
                     GTGFETILNAADQIALGRVKLALCVGTESMSRNPVSAFTHRTGFKMGQVEFKDFLWEA
                     TLDTASNTRMGETAENLARLYGITREEVDSFAAQSFERAVRAWAEGHYRDEVAPVTSQ
                     QWPLENYRARGIALPRHVERFERDEHVRASPLEVLRKLRPAFGQDGVQTGGNSSAIVD
                     GAAAALVASGEYVRAHGLKPLARIVAGVVVGVPPEVMGIGPAPAIRALLRQRELNQSD
                     VARFEINEAFGAQYLAVEKELGLDRERVNVHGGAIAIGHPLAASGVRLTVAAARAARR
                     ARGEFAIASACAGGGQGVGILLQSAV"
     gene            19789..20556
                     /locus_tag="VFQ61_33370"
     CDS             19789..20556
                     /locus_tag="VFQ61_33370"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HET9959445.1"
                     /translation="MQLNASTFIVTGGSSGLGAATAKRFHALGARVVLADINAEQGER
                     LIAELGAGAAFCKTDVTSEPDARAAVELARERFGAVHGLVNCAGIGPAERVNGKKGPH
                     RLESFSQVIQINLIGAFNMIRLAVAAMAQNTPNAAGERGVIINTASVAVWDGQIGQAA
                     YAASKGGVVAMTLPIARELASSGIRVCTIAPGIFRTPMLAGLPAEVQDSLAKSVPFPP
                     RLGHPEEYAALAQQIVENEMLNGESIRLDGALRMAPR"
     gene            20564..>21670
                     /locus_tag="VFQ61_33375"
     CDS             20564..>21670
                     /locus_tag="VFQ61_33375"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET9959446.1"
                     /translation="MANLVTVTQHDGIAVVTLDNPPVNALSHATRLALLSALKERFEA
                     SGVEALVIACEGRTFIAGADIREFGKPPLAPDLPELVEFLDGAPVPTIAAIHGTALGG
                     GLELALACDFRVATENAKLGLPEVALGILPGAGGTQRLPRLIGIRAALEMIVSGIPIS
                     ATEARRKGLVDECVAGDVKELAIAFARRVLAERRPRRRVSSLTVASEDLGAFEDYARI
                     SAERWPGFLAPSRCIEAIRAAGERPFNEGLRRERELFRELVASVESKAQRHVFFGERE
                     VAKLPDLPDGTPTRVIKSVAVLGETASASGIARCFADARIPVALFGHEPSQSERVLSA
                     VREDYLSAVAQGHLSRAECDARLQLIRSTQSEEDA"
BASE COUNT         4008 a         6634 c         7039 g         3989 t
ORIGIN      
        1 ccacccaaac cgccgcgcag gccccccaat aaacagagcc cgtcgtcgaa tcacgctaag
       61 cggcaatcac tcggtccatc ctaccatcac gccgcgcgca cccacgcgcg gcgtgatggt
      121 ctcggccatc ccaggcacga gcctctcaat ggctcagtag aagttacaag tagccgtagt
      181 ggtcaactgg caattgccat ccgagcaagc tcgcatggag tccgcattga gagtccaatt
      241 cgtgtcggag ttccgtacca cactagcgat ctccccgctg ccgcgcagct ggccttgaac
      301 ctgggaaagc atgcagtaga agcgctgaga actagagatc gtttgcgagt tgtaacaact
      361 cgtattgttc gtacaaaagt cggtccattt ctgactcagt tgatacgatc cgaaaggcaa
      421 aaagttcttg tcggcgagcc ggacgcagga catcttgccc cagataccgt tgccgccgct
      481 atggtacgcc tcgatctcgt agcctggaac agatcccggc aggggataaa cccacacgcc
      541 gtcgcgatag ctgtccgctt ggcccttgaa tccagtcatg tagcagaact cattgggatc
      601 agtcagaacc caggtatacc cgcttaccca gcccgtgtca tacggcaccg agccagaaga
      661 ggctccgagt tctgagtact tcacgcacgc ggcctgaacc gcgacgctgt gttggttcga
      721 gtgaccgctg agcgtccaaa agccactgga gtgatcgaga gagacccatt cctgatcacc
      781 tctgaatttc ccggtgattt tcgtaataaa gcagaaccct tccgaaacgg gcagcatttg
      841 ttgagtggcc ccgttctggg tccaagtgta ggtgctgtag atctcctgct tcgtctcgct
      901 aatggcatcg ttcggctccc ccgcttccgg cgccgccgag caagcggata ctgcacttac
      961 catacccaaa gcggccgcat atcgcgctaa cgcaccccag cgcgtagcaa ccccgacctt
     1021 cccgcaattc ctattcgtca ttggattcct tctttcattg actcgcagtc agcacgcgtg
     1081 ttggtgcgcg tcggcacacg acacaacgca ccgcgtgaac aagtggactc tgcgaggagt
     1141 tggctcgtta tggcaaaggt catgcctttg gggacgggtc gcggtccgcc ccggaattcc
     1201 cacgcttgca tgcagaaatc cgggtcctgc agagcctgaa tcacgagcgt gtgcagtcca
     1261 ctagtgattc atggactgct ccacctgctc cacgaattgg gtacacgtgc tcgcgctagt
     1321 ggagggagct ggtcggaata ccgattgtct ataccgcgac gtgatgacgg cgatggattg
     1381 gggcccactg tagcgccgac cgaatagcgg atgcgacttc ccttggctcg aacgccccgt
     1441 gcgcaacagc aatgacaagc ctcggcgtcg ttcgatgccg gagctgatgg cgcaggcgcg
     1501 cttggcccgt gagagtccga agccgtcgct tggaggggca ccgagctgaa cgcgaggagc
     1561 gcgtcaaggc cattcaccag atacggaaag tagctcgagc acgtagccat cgcgtgacgg
     1621 tcgggctact ctgatcgcga tgggtcgcaa tctattctgc ggtttgggcc tcttgttgct
     1681 gtgcatcagt tgcggggaca ccgaggaaag tccggctcct gctcacggcg gcgctaacac
     1741 gtccggtact ggaggtatgg ggggagacgg cggaagctcg ggtgagggga gcatccgcgg
     1801 gggcagctcg aacggtggct ccaccgaatc cggcggacaa gcgggggcgg acggcggctc
     1861 tcgagagccc gccacccact ccggtggtgc tccggaaagt ggaggctcag ggggcgttgc
     1921 cggcgcaact ggcagcgaac cccaggcggg tgaaccgggc gttgcctact gtgaccgggg
     1981 gtttggtgtc tataacgaca agggcgttgg cgctggctgt caggaccctg gattagcgga
     2041 gtctctggct tgcccggcga agttttgttt gacccgagtt ctcgagaatc gctggcagaa
     2101 agaaggagac ttctcgacgc cctcgcactg cagcatccag tgcgatcgag atacggattg
     2161 tggagacggc tttcagtgtt gccaagccac caatcaggca ttctgcctcg acaaccccac
     2221 cgggaacttt gcgatcttgg gctgtgccga agtttgcccc gataacttcc tgggctgcgg
     2281 cgagggccaa acctgctgcg agtccctggg taagatctgc gtgtccaacg attgtcatgg
     2341 agtctgcttg ggacagtgag gatctgactc agggcgttcg acctcggatt cccatgcatc
     2401 gttttgaccc acgtttccgg acagttctgc ccgatctgtc cgatagtggt caagctgtga
     2461 actctgtcga tggtgaggaa tcatggcgcg ttctatcttg cggcgatggt gttccagatc
     2521 gtgcgcgcgg tacggcggct gaatgaaatt ggactcgaag tgcgaaccaa gcggcttctc
     2581 gtgttcatcg cgggtgtagt ggcagtgaca tccgcgttcc tgcctcgcgc ggggcgcgcg
     2641 gactccgcgc gaggaacact cgtgctccgt gtttccggct gcaagcacga tcgcgggaag
     2701 gtggtcgcgc gcatatttcg tcgcagcgac aacgctcccc ggggccccgg ttaccgttcg
     2761 acggccggaa agattcgcac ggggaagtct gtgttggagt tctcggatct cccccacgat
     2821 acttacgccg tcttcgtctt tcacgacgaa aacgcgaacg gaattcccga tcacaatctg
     2881 ttcggactac cgagtgagcc gctcggtttc tcacgaggtt accgcgcgac gctgttttcc
     2941 ggaattccca gctttcgaga tctgagcttc cgattcgatt ccaataccaa gcccttagtc
     3001 ctgcaggtag attgacagac gtaagccaag gggagccgca ccatgcgcga cgacccgtgg
     3061 gctcggtgac gatgcgatcg ggccgctccg gcagagagaa gcgcctggtt catggcggtt
     3121 gcggcgcccc tctcgctggc taaactaggc agaatgtacg ttgcgtttcg gcggtccggt
     3181 acacggcgtt actcagtggt cataacgttg ccggggcaac cctcgcgcgc gttggatcct
     3241 gcgcccgggt acgatgacga tatcccacac gatctcgtgc actacgtggt cgaggctgag
     3301 ctgggcctga agtacggagt gtacggatcc gccgcgaggg gtggtggcat gttcatctcc
     3361 acctcggaac gagacgtaca cgcgcgcgca cgtacgaggg aacagcgcaa gcaacgcaga
     3421 cgcgagcgag cgctgcgtgc gcacgacgcg agccatgggg ccgaactgat gcggtctgag
     3481 cgtctcgccg cactttgcga catcacttgg cgacggcggc acggacagca tcccgattta
     3541 ggtcgggtgg cccccacggt tcgagcagaa gacgtagctg acgtgcaacg cgtggtcgcc
     3601 cgcttggact cggtcgcgcc gctctggcga gacctccgcg tcgaggacga actcgttttc
     3661 gattggcctc acgtcagtcc gcggcaagag cactcatcga gtcgcccttg aagttctgcc
     3721 gtgaattgcg ccgcgcctgc cggcggagct ttcaatgctg cactcggcgt gcggacgtct
     3781 gtgctcaggg gacggcattg ctcatcgtcg gtattctcgc ggctgcttgc ggtcccggca
     3841 tcgtacgcgc gcccggttgt acgcgagagc cagtggtcga ccgtcgcact ccctattata
     3901 ccgagacgtc acgctgggcg gcggtgggcg agaaatgctt ggaagcggaa gaggaccagg
     3961 ggcttatagc gtacgacacg ttcgtcaatc gggcgttggg acttcccccg aagggcgaca
     4021 ctctttgggc tctccgcatc agcccgtcgt ttggctcaga gtcagccttg gccctccgtc
     4081 agacgccgca aggcgagtac gtattgcgat tgaccaagct ctcgacctcg gtctggtacg
     4141 atatgatggg ccggatgcga gagcaacaac gcggaacgct ctcgctcgat gagcgttggc
     4201 aagagtctgc cttgaagagt gtcagtgcga agcgcgtcgt tcaggagcgc tacctggacg
     4261 ggaagaccgc gaaactgctg cttgcaactt gggagacgtt gcttcgtcgc gagcagatga
     4321 cctcgacctc cgagataggc gtcgtgacct tggatgggac cggttatcgg gtttgcccta
     4381 ttccgcgtct ggttggcgct cgggcgccgc atcacgaaag gcaggcaaag acgcgcaact
     4441 ctgttcgact gcgaggagtc tcgggtagcg ggagacgtcg catttgaagc gacgcgcatt
     4501 gaagagctgt gggatcaagc aacagtccgc cagtcccggt gagtcaccat gacagaagcg
     4561 gccgggcggg ccgatcgcga gttccgcttc gagggtgctg aggcccagct cgacccagtg
     4621 ctggtaccag cgcgtgcgcg cgtcgctgtc gagcccgagc tgttgcgtca tgtagttgag
     4681 gacgcgcagg ttgttcaggg ggtgaatctc gcaagcgacg gtgagggcga gtgagcgcac
     4741 tcgcgcgcgc ccgaagggat tgctcgggag cagcgggggc tccgggtgaa tctcatccaa
     4801 gtactcgatg atggcgagcg actgggtgat tcgtcggtcg ccatcctgga gtacgggtac
     4861 caacgacgcg ctgttgagcg cacgaaactc cgcagcgaat tgctcgccga ggccgcggct
     4921 caggtgtacc gatacgtact cataggagag gcccttcaag ttcagggcga tgcgcactcg
     4981 ataggacgcc gagctccgga agtagttgtg aagcttcatg gtgtggcctc cgcgtgggcg
     5041 gggccgatcc taatcgcgag ttcgccgacg ccttcgatgt taccccgcag ctcgtccccc
     5101 ggcttgaccg ggccgacgcc ggcgggtgtg cccgtgtaga ttaggtctcc aggacgaagc
     5161 acgaaaagct tcgagagctc gacgatcaga tcggcgaccg gccagagcag ctgttcgaga
     5221 tcggcattct gccggtccac gccgttcacg gacaagcgga tgttgccttt cttcgggtgg
     5281 cctatgtcag tggcgcgccg aatggcacta atcggcgccg aatcatcgaa ggctttgccg
     5341 acgtcccaag ggcgtccctt gtcgcgcgcg gcgagctgca agtcgcgccg agtcatgtcg
     5401 atgccgacag catagccgta agcgtgctcc aatgcggtct cacgcgaaat ccgagcgccg
     5461 cctcgtccaa tcgcgaccac gagctcgatt tcgtgatgga ggttttgcgt ggcgagcgcg
     5521 tacgggatgg tggcgccgtt cgcgacgatg gcgtcggccg gcttggtgaa gaagaagggc
     5581 ggctcgcgct ctggctcgaa gcccatctct cgcgcgtgct cggcgtagtt gcgcccgacg
     5641 caatagatgc gatgtacggg gaacgaggcg tctgcgcccg cgatcggaac agtggacacc
     5701 gggggcgccg gaaatatgta gttcatgacg attctcctag gccagcgctg tccgtcaaaa
     5761 aacgtagaag tgcccggcca agacgatgag aggccagtgg ccaccctcga acagtgcttg
     5821 tttcaacagg aaggggtgtg acgcgggggc gcctgccaat tgggcgagct tgcccaggta
     5881 gagagcttct ccgagggctc ccgccgcggc gcggatgagc ccggcatcgg cgcagcccag
     5941 ccgcgtagct tgagcggcgg cagtgccgtg gtgttcagat atggaatctt gcacgaaatc
     6001 cgacaatgca cgcaaccgtt ggccgctacc cggagtggat actagctggt ttcggagccg
     6061 ttctcgctct ctttgttcgg cgtcccacca acgacggtcc cagtctggat gcgcgatgat
     6121 tcgggcggca gctgcaaaat ctttgacggt ctcgaacgat aactcctgct cgagacctag
     6181 cccggtgagg tactcgcgga cctgctcgcg ctcacgctgc cctagcggtt cgccgagcat
     6241 gaacagccag tcactcacgg gatggcgtcc tcacgccata tccccagcgc cttctgcacg
     6301 ggtcgatcgg aatagctgaa caacactgag tcggcgtcgg cctcatgctg gactggacac
     6361 caagaaggca ccacgaacac gtcctttggg gcccaatcga atagctggtc gccgatgcgg
     6421 ctgcggccgt ggccctcgac cacgcaatac acggtgccgt cggtggatcg gtacccccgg
     6481 ccgcgaaagc cctctggcaa taactgaagg taagcggcga tcgtcggcat ggggtagcca
     6541 cccgtcaccg ggttgctgta ctgcatcttc acgccgtgaa acgagctgag ctcagcttgg
     6601 cggtgcagcg cgcgcagcgc ggcgcggcta cgagcgtagg ggtacgaaag gagcggagtg
     6661 gagagtgatg gtgattgata ctcgtagggc agtaggccag ctccgtagcg cgacagcgca
     6721 tcaccatccg gtcgagtcag cgcttgcttg ggctcggcgg agcgctcggc gaaccccgcg
     6781 tcgaagaagc taatcatggg gatatctagc ccgtccaacc acacgacggg gttatcgccc
     6841 gggttcgcgt ggtcgtgata ggcccacgac ggggtgatga tgaagtcccc cggttgcatg
     6901 aaagcgggtt cgccgttcac cgccgtgtag gcgccagtgc cttccaccac gaaacgcagc
     6961 gcggattggg tatggcgatg acttggcgcc cgttctcccg gcaagatcag ctgcaagccg
     7021 gcataaagtg tgcgcgtgat cgaggaagcc ccgcgcaaac cgggattctc catcacgagc
     7081 acacggcgct ctgcttcctg cgcgctgatc aggcggcccg cctccatcag gtggggtcta
     7141 accgcttcat agcgccagag cgccggtacg cacggggagc ttggctgcgg cggcaccagt
     7201 tgcgcgagtg attcccagag tggcgtcagg tgatcgcgat cgatccggcg atagaactcg
     7261 gcgcgggcat cggacgcttg cgcagaagag gtgggtagtg cggcacgcat cccattaccc
     7321 ctggggtatg tcgagagctg gaaagcggag cccgagcgac tcgaccactc ctcgtgcacg
     7381 cgccaggaag tcctctcgca tctgttcgtt cgtgcgctct ttcaagccgt acttacgata
     7441 gagctcgttg ttcttggaac cggcggcgcc gaagaactgc ggcaagaatg gggcgtagtc
     7501 gtcgatggcc tgctgcaccg cgcgccggcc ctcttcggtc ttgcacagct ccgtgcaaaa
     7561 gtccttgccg aattgagcat ggaacttctc ctccggcata gtgactcgtc cgatgttgcg
     7621 gagcgggacg aaactgcaat gcaggagatc ctccacctgc agaatttctg ccagatctgc
     7681 cagcatcttg atcacacaga actccggcca agtcttcatg gcaaaaccga agatggtgag
     7741 tggacgtttc acgttcggat cggtggcgct cggtggaaca cccagttcgt cggccaggcc
     7801 tttgaagcgg acgtggtggt ggtactcctc catcgcaatg cggcagctga gccacttagc
     7861 gtacggggtt ggagccaggg cgatcgcagg ctcatcgaat acttgcgccc catagagctc
     7921 gttgaccgtg tggctcacca cgatcttcga gatcgcctcg cggtactccg cgggttcgct
     7981 cgccagatcg gccacggtct ttacttcgtg atatgcagtc gtcattgcgg aattctccta
     8041 aaacacaggc gccacagaca ggcggactga ttgggattct gcccggatga ccctggactt
     8101 cgcgcgaaat ccgggatcac gaaacgggtt cggtgttgaa tgcctccaag tcagtcgtca
     8161 gctcgactag gcggttcgtg aagtgctccc gaacgactcc cggcagcgtc gctgcgccat
     8221 cgtcggtctc gggcatcacg aagtgcgcta gcggacgggg ctgcgagaag agaaacactt
     8281 cgcgaccgcg tactccgaag agctgaccgc tgagcgtttg agcctcgggg ccgcagagga
     8341 aggcaacgaa gtcgcctacg aagcgggcag aaacccgcag cgctcgctct ttgtactgcg
     8401 cttgggcggg gtttgcgggc tggatcgcct cggtcacgcg ggtcgcgccg aacggcgcca
     8461 cggcgttgca ggttacgccc gagcgcgcca tatccatcgc ggtcacgcgc gtcaaaccca
     8521 agagcccggc cttggcacta gcatacgcgg actggccgaa gttgccgtaa agccccgtag
     8581 acgaggtgat attgacgacg cgacccggcg cgcgacccga cttggcttgt tcacgcaagt
     8641 gcggtgtggc cgcggcgagt acgtaaaaag ccgcgctcaa gttgctttgc aggaccgcgt
     8701 tccaattggc ggggttcgct ttgaagatga gactgtcgcg caagatggca gcgttgttca
     8761 ccacgagatc gatggcaccg aagcggtcca ttgcgagggc cacggcctcg gcggcgccct
     8821 ctgggctcga gatgtcggag ctgaaggcgg cggctcgagc gccgagctct tgcgcggccg
     8881 cgtcggcgat cgacggatcg ggagccgttc ctgaaatcga gacgccgctg tccgcgatca
     8941 cgacgctagc tccacgcgcg tgcagcgcgc ggctaatcgc caggccaatc ccacgcgccc
     9001 cgccggtgac gagcgcgact cgttcgttca atgaattgct tgcgggcgtg ttgaccatga
     9061 ggcactgact cctgggctac caagtattga aggcgtcgtc ggattggctg taaaaggcgt
     9121 cggcgtgcac gtcgcgtgtg gccacgccct tcccgagcag ctcgtgttgc accgattcga
     9181 ccatgggtgg tggccccgcg acgtaggctt tgtagccgct gagttcaccg aagtcttcac
     9241 tgacggccgc agcgaccgtg cccgttcttc gtgacgtggg tgcgctgggc tgcgagagga
     9301 tgacctgtac gtgcaggttg ggatgccgtc gaacgagcgc ggcaagatgc tcttcgagat
     9361 agacgtctcg ctcgtcccgc actccgaagt agaggtggat gacgcgttcg ggatcgcttc
     9421 ccaacgccgt ctcgacgatc gattggatgg gggccatgcc gctgccaccg gcaacggcca
     9481 ggatcggacc gcggtggttt gcgcgtaggt aggcgttgcc gaacggcccc gcgacgacga
     9541 cctctgcgcc cggcctcaaa ctcgtgaatg cgtatccgct ggcttgtcca cgcggcaccc
     9601 gtcgcacatg gaatgctagc gacggatgat cggggcaatt ggccatcgag tagctgcgct
     9661 cggggatgcc cggggcgaac ttgagccgtg catgttgccc ggcggagaat ccatacgggc
     9721 ctccgctctc aatctcgagc tcgaggcccc gaatatcgtg cgtgagctcg cgaatccgag
     9781 cgacgcggca gcgcaggatg cgcgaggggt gcagcaccat ctcgtccgct tcgagcggcc
     9841 gcaccgtgca gtcaccccag acctgagttc gacaggccaa gatcaagttc cgagcgcgtt
     9901 cctcagcgct gagcgcatac tctgaatagg gaagatccag gacatcccct tcgacgagtt
     9961 cgcatttgca cgcgccgcag ttcccggact ggcaattgtg cggaaacggg acgccggcac
    10021 gcagcagact ctcgagaatg gtttcgcccg cgggcgcggg cgcgctttgg ggtggattag
    10081 acagtactcg aatccgagag ctcatgagtg actcccgtgc tcggagggga tggctttctc
    10141 cgcggtggag tggcgcactg gtgccgacga ccgcgcgtgc gcggcccaac gcgaacgtgc
    10201 tccgacccag gtacgggaga cgagggagac gacaccgcca ggtagaaaca gcaccgcacc
    10261 gatgagcagc gcggcgtaca ccgcatagga caagatgacc ggggccgtac ttggcatccc
    10321 gctcagggtg gcgattcgac tgaggccatg caccagtaag taaaccaaag cgctgccgac
    10381 caaagctccc gaaattctcc cgaggccgcc cacctcggcc atcacgatgt actcgattga
    10441 caacaacacg gggaacgagc ccggagccag gtagcccatg taaaaggcat agatgcctcc
    10501 cgcgagcccc gcgaaacctg ctgacagtgc aaacacagcc aacttgtact gcccgactgc
    10561 tacccccgat gaagccgccg ccacttcgct cgaggacaaa gcacgcaggg cgcgaccggg
    10621 acgtgacgcg agcacgttgc tggagatcac gaccaccagc accagcgcca gactacccaa
    10681 ataggcgtag ccgcggaccg aggtcagggt gaatcccacc acggcgagcg gcggaattcc
    10741 ctgcaagcca acatcaccac ccgtgatcga gagctcgctg atcgaggaca ggaagatcag
    10801 atgcaaggcg agggtcgcga aggcgaggtg atggccgcgc aacaagagca gtgggacacc
    10861 cacgacggtg gcgattgccg ctgccgccat cggcgcgaac accaggccca cccaagtggg
    10921 atatccatgg gtcgccaaca ggcctgccgc ataagctcca caagcgtaga aggcgccgtg
    10981 accgagcgag acctgaccgg catgtcccat cagcagcgtg agcccgacgg tgacacaggc
    11041 cgtcagcatc aagagcacgt agacgccgag cgtgctctcg tcgagcaacg aggcgataac
    11101 ccaaaggccc gccgcgagaa ccgcgatcgt tcgggagcgt gcaacgctcg aggtcattct
    11161 tccaccgcgt gaagggactc gcgtcgcgtc gcctgcgcca ccagaattgc cagcgtgagc
    11221 aacaacgcca aggagctctg gtaggaagcc ttggcatatc ccgcaaccat cgactcggcc
    11281 acaccgagca ccagcccgcc cgcgagcccg agcacggggc gcttcaagcc ggcgaggatc
    11341 gccgaggcga agccgttcac gaacaaggag acatcgctgt cgtaagagat gggctgcagc
    11401 ggtgtcagca gtactcccgc gataccgccc agcgccccac ccaacgtaaa gccgtacaac
    11461 cccattcgct ggaccccaat gccttgcagc gccgcggcgt agggattcga ggcacaagcg
    11521 cttagcgcct tgcccgcgta agtccgttcg aagaacagct cgagcgcggt gaagacgagc
    11581 aacgtcacta cggtgatgag gagatattga ttgggcacct ctagattcga aaacccctgt
    11641 gtgcccgcca agccggggaa agagcgcggc tggtcgcccc acacgagcac ttcgacggcg
    11701 tagctgaaca cgcctacgcc gagagttgca atcaacgaag cctgcgggct gatcccgcgc
    11761 ttgccgatgg ccacgcaccc tgtcaccaag ccgaccagcg cggcggcgcc gatggccaag
    11821 agctcggaca ctccgtgtgg caggcccact ccgagcagag tcgtggccgc aaagcccgcc
    11881 accacgctga acatgccttg cgccaggttc accacgcgcg tgacgcgata aacgatgatg
    11941 attccgctgg ccacgagcgc aaagccgcaa ccaacgcaga tgccatgtac gaggtaggag
    12001 aggaacgcgc tcacgagggc ctctccaagg gactccccaa gtacgcgctc tccacgaatg
    12061 gcgaatcggc gagctccttc gcggagccct gggccaccac acggcccgtc tcgagcacgt
    12121 aggcccggtc gcagagtgag agcgccacgc gtgcactctg ctcgaccagc agcagcgtga
    12181 gaccgcgcgt ggcgttcaag gtcgcaagcc gatccgcgag ctgctcgacg atcagagggg
    12241 cgagcccgag cgacatctcg tcaatcgcga gtacgtcggg gcgtgccatc agtcctcgcc
    12301 cgatcgccac catttgctgt tgcccgccgg acaagctgcc cgcgcgttgg cgccgcacac
    12361 gcgcgagctc gggcaagagc tcgtaaatcc aggcggggtc ccgctcacgt acgcggaacg
    12421 cacccaggcg caggttatct tcgacgtcga gatcgggaaa gagctggcgc ccttcgggca
    12481 cgtgcgccca gcggccctcg acgcgaaggc tgccagctgc tttggggatc acgccggaga
    12541 gcgcgttcag cagggtggat ttgcctgcgc cgttggcacc aatcagcgcg accttctcgc
    12601 ccgcagagac ggtcagagaa acccggtcga gcgcggtggc ggcgccgtag cgcaccgtca
    12661 aggcttccac gcggatcatg acgcgggggt ccctcccaga taggcggcga ggaccgctgg
    12721 atcacgccga atggcgctgg gttcaccctc cgcgatcacg cgaccgagat cgagcacggt
    12781 aatgcggtcc gatagactcg tgacgagggc cacgtcgtgc tcgactagca gcacggtgag
    12841 cccacgcccg cgcacctccc ggatcaagcg ggcgaactgg gtgcgttcag cggcgtgcag
    12901 acccgcggcg ggttcatcga gcaatagcag cttgggatct gcggccagcg cgcgcgcgag
    12961 ctgcacggtg cgttgttgcc cgagcggtag cgattcggcg cgctgctccg ccaccgcgct
    13021 gagcccgacc tgatcgagca gagcgtcggc acgggcgcgc agctcctttt cctcacggcg
    13081 acagctcggt aggcgcaacg ccgccgcgag aaatccatga cgcgatcggc aatgtgcgcc
    13141 gaccatcacg ttctctcgca ccgtcatgcg tcgaaagatg cgatctccct ggtagacaat
    13201 cgcgaccccg agccgcgccc gctggtcagg ccgatatcca tccagggctt cggtcccgag
    13261 cgatacgcga cctgcgctcg gcttcaggtg gcctgcgatc aaatggaaga gagtggattt
    13321 cccggcgccg ttggggccga tcacgccacg gagctccccc ggttgcacgc gaaagctcac
    13381 gtcgcgcacc gcgtatacgc caccgaaacg ccggctgaga ccttcgacgt gaagcatcat
    13441 cactgttggg caagcgtttg ggagagcttc tcctgggccc actccgtggg tacgagtttc
    13501 ccttctcgca cgacgttcac ggaaatgtaa tcccgagtca ggccggagtg gtccgtcggc
    13561 gaatatcgat agtggccgtt tggcgtcaac aggctgagat tctcgagtgc ctgctggatg
    13621 cgtgggcgct cggcgctacc agccttcttg atggcttcga acagcagcag acacgcgctg
    13681 tagccgtcct gtgcgaattg gggaggcggg tagccgtggc gttgttgaaa cggcacggac
    13741 attcgttgga tgacctgctt ctgcggcccg tccgcgaggt agtctccgac gactccaatc
    13801 gcgctcagca cggtcactcc ttcacccgcg gtgccgaccg gatcgatcca cagcttgctt
    13861 gcttgggcgg gcgtcatgaa gagcggcatc ttgagcccgg ccgcggcgta ctgcttggtt
    13921 gcggtgacac ctgaggggcc ggtgccccag aacacgaacg cctgtgcgcc cgagcctttg
    13981 agatgcgtga agagcggact gaagtcgttc gtggtggtct cgaagacctc atcgcgcacg
    14041 atctgcacgc catacttgct ggcgagtgac ttcgtcgtgt tgtacccgga gatggcgtat
    14101 gcacctttgg tgtcgtgggc cacggcaatt ttggtgatct tcgtggcctg catgtactcc
    14161 aggtatcttt ccgcgtaggc attggccagg gcggggacca cgaacacgta cgatttgatg
    14221 ggaatgacct gctcttctgc cggagtcagg gagagatagg gaatgccatc ccgctgtgcc
    14281 attggcaaag tggccagcgc agagttcgag aatacggagc cgatcacggc cacgacgttc
    14341 tcggccttga gctcgttgta cgcgagcacc gatttgtccg gtaaggtctg atcgtcgcgc
    14401 gaaatgacct cgaggcgttt gccgagcaag ccaccgctcg agttgatctg ctcgatcgcg
    14461 agctccacgg ccttcttgtc ttcgctgcca agagcggagt acttccccgt caaggacgcg
    14521 atcagcccga cccgaatggt ttccgatttc ggggtcgcag cggcgttggc ctcccggtgg
    14581 agttctttga ccggtgccgg ctcagtgctt tttcgacacg cactgaccat caacactagc
    14641 gcgaggctgg cgagcgctcg gcgggtgcat accatcgaca ttctccagtt cgattgaggg
    14701 actgagtagg actcaactag cgtgcgttca cggatcggcg agagtgacgc caaagtacgc
    14761 catattactg gcctggctgc gcgccggggt cgtggcggtg ggcgcgaggt acgtatcccc
    14821 atgacgatat ccgagttgga atcgcgcgaa ccacgccgcc acattgtagc tgagttgagc
    14881 ctccacgagc gaggtcggat cggcgcccgg tgaattgtct agcgcgcgct gatatcggac
    14941 ggtcgcgcgt actttaccaa tcccgagcgg cttcggcagc atataccctg cggaggctac
    15001 ccagaagtct ttccaacgtt gaaagtcgcc gccgtacttg tagtaggcac ccacgagcga
    15061 gaccgttccg gcgtcggcga tattcttctc gaccgtgaga tccgccgtca cgttgtcgta
    15121 gtcatcggtc aacgggggcg tgggcggggt agtgaccggg acggtttgaa cggacccctt
    15181 cttttggtac tgacccccga ctccaaagga caccaagtct ttggtcccga agtaagtggt
    15241 gcgatggtaa aacgcgggtt ctccgttgag caagctgact tggagccgcc cactgagtag
    15301 cggattcagc gccggatcgt agagattgaa agcaccaaga tagtacttca catggccctt
    15361 cagcggcgcc ccccagaccg tcacgccgac gtcgcgcccc gaggcccccg ctttgggcag
    15421 ggcgggtggc gcgaccaacg gaaagaaacc cggtaaaaag aactcgtcca tgccccaagg
    15481 gccgcttggc gcgaagcggt ccacgacgac gagcatgcgg cccaagtaca cgttgaattc
    15541 gggaatgggc tcgatgcgcg caatcacatc atggggcgtc acgttcacgg tgcccggcga
    15601 gcccaccgtc cccgggtagg acatggtgac actgacttgc cacttgagat atcgatggat
    15661 ctgaccagac atatagaggt cagccgtcaa cacctccgag atatcatcca ttcgcgaggg
    15721 gtggtcggga ttctggaact tcaggcccgc ctgcatgcgc gcgccataat cgatgttcgg
    15781 aatcgccggc ggaaacgcgg gcgcgatcgg gcccgcgggg gcttcaccat caggaattga
    15841 cgaagccggc ggccctgccg gaggggtgcc cgcaggcggg ggggcgggtg cggtgctggg
    15901 ggcagccggc gcagccacgc tgggtggagc cgttggcccc ggcgaggggc ttgcagcagg
    15961 cggcggtgaa tcggcgcttg gcgctggtgt gtcttgagga tgctccgcac caggctccgc
    16021 gacgggcgca ccgtcctggg gtggcgtggc ctgttgagct gtcgcgctgc tgacccacag
    16081 gaatgggccg aggtgcaggc aagctctcag aagccagtga tgccgtaccg gtgtcgagcg
    16141 tccactcatt tccattctcc cgagcgctgc tctgcttgtc cgagcggttt gtccgagctc
    16201 tgctccgcgt ctatgcaccc tcgtaaagct cggtgctctc gcggtgttcg cgctgaacgg
    16261 ccgtcgccgc ccgttaacca acgagagcta ctatagtata cgcgagcgcg agatgactat
    16321 cataatcgcc gcttccacca gcggtcctcc gctgagcgag acgcgccatg tttctggcaa
    16381 atattgggcg atcggaaagt tccggaaagg cggacgcaca tggatccgct ggacaaccag
    16441 agatctacta tagtacacct tggtccatgt tcgcggcgtc gcgaggttcc ggcgagctcc
    16501 gctgtcctga cttcagaggg cgattgagag tgtgccccac ggacgagatg ctgtgtggtg
    16561 acggcgttct acggttgagt cacgagctgt gacgcgattc gaatccctgt ccaacgatct
    16621 attcgcgctg cctgccgtcg agctggtgcg acgcgaacgg gatcagctgt tgcgttctcg
    16681 tcacgcactc cgcccttatg cgcgctgcag tggggagttc ttggagtact gggcgcgctg
    16741 cgcgcccgag cgggagttcg ttctagagcg caacgagacg gggtggcgtg ctctgagcta
    16801 cggagagatg ctcgaccggg tgcgtcgcgt cggagcgtgg ctgctcggta ccgggctctc
    16861 cgcgcaacgc ccggtggcga tattgtccga gaacagcctc gaacaagcgg tactgactct
    16921 ggcgtgcttg cacgttggtg tgccggtagc gccgatctcc ccggcatatt ctttagtttc
    16981 gaaggacttc ggcaaactca ggagcatcct gcggaccttg cgtcccgggc tcgtctacgt
    17041 gagtgatctc gcacgcttcg cgcccgcgct gtccaaagta cgcgacgtgc acgatggctc
    17101 gatcgtgagt ggggagcgat acccgggtga cggtgtgttg gcattcgata cgcttctccg
    17161 cgcacgcgac gacgctgcgg tggagcgtgc gttcgaggcg atcactgcgg atacgatcgc
    17221 gaagttgatg ttcacctcgg gttccaccag tgagcccaag gccgtcatca acacccaacg
    17281 aatgttgtgc tcgaatcagg aggccatcgc gcaaatttgg ccattcgtga atcgcccgcc
    17341 cgtgttgctc gattggctgc cctggcacca caccttcggc ggaaatcaca acttcaatat
    17401 cgtgcttcgc aacggcggca cactctatat cgacggtggc cgcccgttgc cgggtgagtt
    17461 cgagcagacc ttgagcaacc tgcgtgaggt cgccccgacg ctctcgctga atgtgccacg
    17521 cgcttacgac ctgctggtgt ctgcgctgca gcgtgattcg gagttgcgga gccgcttttt
    17581 cagccggctc gagctaatat tctatgcggg cgccgcctta ccacagcatc tctgggaagc
    17641 cttgcgcagc ttggcccggc aaacgctggg gcacgatcta ccgctggtat cttcttgggg
    17701 gctgaccgag accgccccta cggctacgat cgggcacttc tcgccggatc gggccgggat
    17761 catcggtctg cctgtgccgg gcagcgaact gaagctcgtt ccgaacgggg acaagacgga
    17821 ggtacgcgtt cgaggcccca atgtcacgcc cggttacttc aggcgcgaag aattgacggc
    17881 gaacagcttc gacgacgagg gcttcttcag aacgggcgac gccgtgcgct tcgtggatcc
    17941 gaagcatccg gagcgcggtc tggtgttcga tggtcgcatc ggcgaggact tcaagctcag
    18001 cacggcgacc tgggtcaacg tcggcgcgct gcgcctcgag gccattagtg cgctagcccc
    18061 cgtggcgcag gacgtagtcg tgacggggca cgatcgcgac gagattgggc tgctgatttt
    18121 ccccagcatc gcgggttgtc gttcactttg cagcgatctt gcggctgagg cggcgcctgg
    18181 ggcgttgttc gagcacccgg ccgtgcgggc acgagtggcg gcggggcttt cagcgctcgc
    18241 ccgcgcgagc acgggctctt cgacgttccc gcgccgagcg ctcgtgctgg cggagccggc
    18301 gtgcgccgat gcaggcgaaa ttacggacaa agggtacatc aatcagcgcg cagttttgac
    18361 ccgacgagcg gcgctggtcg acgtgcttta cgaacagccg ttgcaggccg gggtgatcac
    18421 cgtctcccca gacgaggtcg cgtcgagtgg ccgcgcacag gtctttcggg gccgctctgc
    18481 ggctccgcca ttggagtcga acatgtacga agcttcggac cctttcgcgc tggttgctcg
    18541 ttacgacgat atctggttgc tcggcggcgt acgcaccccg tttgccgact acacgagcat
    18601 cctcagcggc gtttctgcca cggatctcgg aatccacgtg gcgcgcgcgt tgttccaacg
    18661 ctcggacgtg ccggcgcgcg acgtcggcgc cgtgatcgcg ggtaacgtcg cccagtcgag
    18721 cttcgacgct ttctacctgc cacgccacat cgggctctac tcaggcgttc cactcggagt
    18781 gccagcgctg ctcgtgcacc gcctgtgtgg cacgggattc gagacgatct tgaatgccgc
    18841 cgatcaaatc gcgctcggtc gagtgaagct cgccttgtgc gtcggcacgg agtccatgtc
    18901 gcgcaacccc gtttccgcct tcacccaccg cacgggattc aagatgggtc aggtcgagtt
    18961 caaggacttc ctgtgggagg ccacactcga tacggcgtcg aatacaagga tgggggaaac
    19021 ggcagagaac ctcgctcgcc tctacggcat tacgcgcgag gaagtcgact cgttcgcggc
    19081 ccagagcttc gagcgcgcgg tgagggcatg ggcggaaggc cactatcgag acgaggtggc
    19141 tccggtgacc tcacagcagt ggcccctcga aaactatcgc gcgcgcggca tcgcgctgcc
    19201 tcggcacgtc gaacgcttcg agcgcgatga gcacgtacgg gcgagcccgc tggaggtgct
    19261 gcgtaagctc cgccccgcgt tcgggcagga tggtgtgcaa accggtggca acagctcggc
    19321 catcgtcgac ggtgcggctg cggcgctagt cgcgtcaggc gaatatgtac gtgcgcatgg
    19381 tttgaaacca ctcgcgcgga tcgtggccgg cgtggtcgtc ggggttcctc cagaggtgat
    19441 ggggatcggt cccgcacctg cgatccgcgc cctgctgcgg caacgcgagt tgaatcagag
    19501 cgatgtcgcg cgcttcgaga tcaacgaggc gtttggcgcc cagtacctgg ccgtagagaa
    19561 agagctcggc ctcgatcgcg agcgggtcaa cgtccacggg ggcgccatcg ccatcggtca
    19621 cccactggcc gcttcggggg taaggctcac cgttgccgcc gctcgtgccg cgcgtcgagc
    19681 cagaggtgaa ttcgcgattg cctctgcctg cgccggcgga ggtcaggggg tcggtatcct
    19741 attgcagagc gcggtctgaa ccacatctga aagcatcgag aacaagacat gcaactcaac
    19801 gcctcgacct tcatcgtcac gggtggcagc tccggcctcg gcgcggccac cgccaagcgt
    19861 tttcatgcac tgggcgcgcg cgtcgtactc gcagacatca atgcggagca gggcgagagg
    19921 ctcatcgcag agctgggggc gggggcggcg ttctgcaaaa ccgatgtcac gagcgagccg
    19981 gacgctcgag ccgccgtgga gctcgctcgc gaacgcttcg gtgccgtgca cgggctagtg
    20041 aactgcgcag ggattggacc ggcggagcgc gtcaatggca agaagggccc ccaccggctc
    20101 gagtccttta gtcaggtcat tcagatcaac ttgattggcg ccttcaatat gattcgcctc
    20161 gcggtcgcgg ccatggcgca gaacaccccg aacgccgcgg gcgagcgggg cgtcatcatc
    20221 aacacggcgt cggttgcggt ttgggacggg caaatcggtc aagccgctta cgcggcatcc
    20281 aagggcggcg tagtggctat gacgctgccc atcgcgcggg aactcgcgag cagcggtatc
    20341 cgggtttgca cgatcgctcc agggatcttc cgcaccccga tgttggcggg gttgccggcc
    20401 gaggttcaag attcgctcgc caagagcgtg cccttcccgc ctcgactcgg tcaccccgag
    20461 gagtatgcgg cgctggcgca gcaaatcgtg gaaaacgaga tgttgaacgg tgagtcgatt
    20521 cgtttggatg gcgcgttgcg gatggcgccc cgttgagctc gtcatggcca atctagtcac
    20581 tgtcacgcag cacgacggga tcgccgtcgt caccctcgat aacccgcccg tcaacgcatt
    20641 gtcgcacgcc acgcgcttag cgctgctctc ggctctgaag gagcgtttcg aggccagcgg
    20701 tgtcgaagcc ttggtgattg cctgtgaggg gcgcacgttc atagcggggg ccgacattcg
    20761 ggagttcggc aaacctccgc tcgcgccgga tcttcccgaa ctcgtagagt ttctggatgg
    20821 cgcgcccgtt ccgacgattg ctgccattca tggtacggcg ctcggaggag ggctcgaact
    20881 cgcgttggcg tgtgacttcc gagtggccac tgaaaacgcc aagctcgggt tgccggaagt
    20941 tgcgctcgga atccttcccg gcgccggtgg aacgcagcgg ctcccgcgtc tgataggcat
    21001 acgggccgcg ctcgagatga tcgtctccgg tatcccgatc tctgccaccg aggcgcggcg
    21061 caaagggttg gtcgacgagt gcgtcgcggg tgatgtgaag gagttggcca ttgcgtttgc
    21121 gcgtcgcgta ctggcggaac ggcgcccacg acgtcgcgtg agctcgctca cggtcgcctc
    21181 cgaggatctc ggggcgttcg aggactacgc ccggatcagc gccgaacgct ggcctggatt
    21241 tttagcaccg tcccgctgca tagaggcgat tcgggccgcg ggggagcggc cgttcaacga
    21301 gggtttgcgg cgcgagcgag agttgttccg ggagctcgta gcttcagtcg agtccaaggc
    21361 tcagcggcat gtcttctttg gcgaacgcga agtcgccaag cttcccgact tgcccgatgg
    21421 cacacccacc cgcgtgatca agtccgtggc ggtgctgggc gagacggcat cggcgagcgg
    21481 gatcgcgagg tgcttcgccg acgcgcgcat cccggtcgcg ctcttcgggc acgagccgag
    21541 ccagagcgaa cgcgtgcttt ctgcagttcg cgaagactat ctgagcgcgg tggcgcaggg
    21601 gcacctcagc cgagccgagt gcgacgcgcg gctccaactg atccggtcaa cccagtcgga
    21661 agaggatgca
//
LOCUS       DASTKU010000943         2910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_184726, whole genome shotgun sequence.
ACCESSION   DASTKU010000943 DASTKU010000000
VERSION     DASTKU010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184726"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..450)
                     /locus_tag="VFQ61_33380"
     CDS             complement(40..450)
                     /locus_tag="VFQ61_33380"
                     /inference="COORDINATES: protein motif:HMM:NF037617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959447.1"
                     /translation="MADPVQRKTREAREVLAALGLPSEQQNDRSAWTLLALLALKPNT
                     AWSRAAAPLMGITPIMDFVRDEYGHAYKPNTRETVRRQTMHQFVAAGLVIANPDEPGR
                     AVNSPAYVYQVEAKALELLRTFGVSAAPSPFFTS"
     gene            complement(410..1993)
                     /locus_tag="VFQ61_33385"
     CDS             complement(410..1993)
                     /locus_tag="VFQ61_33385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HET9959448.1"
                     /translation="MGTPAPVMSFTPAVLLNQVDTWRREASARLDPAKRSEFGQFLTP
                     SPIASFMASLFTDFGDEVRLLDAGAGGGALLTAATIAALNRKQRPRIIEVTGFEIDPI
                     LLGYLRRAVDELASSCREAGVDLKGRLVEHDFIEATVEDVDRGLFSRRTPAQYTHAIL
                     NPPYRKLNSKSASRLLLRRAGIETSNIYTAFVALAIQRLAPGGELVAITPRSFCNGPY
                     FKPFREFLLREMAIQRIHVFDSRTAAFSEDEVLQENVIFHAVKGAPQRLVTVSSSPSP
                     RDEPKARQVPFAEIVHPRDRESFIHLVPNAEGQDVATAFAALACNLRDIGLGVSTGKV
                     VDFRAKWALRGDPGPGIGPLIYPTHFAEGRVRWPKQGKKPNGLLDDASTEELWMPSGT
                     YVLVKRFSAKEERRRVVAVVFDPEDVPGKKVGFENHLNVFHVNGGGIGPMLARGLAAF
                     LNSTLTDRYFRQFNGHTQVNATDLRNLRYPAADQLLALGKRLEDPGCAQEELDELVAD
                     VIGFDADGRPCPAKNKRSS"
     gene            complement(2525..>2910)
                     /locus_tag="VFQ61_33390"
     CDS             complement(2525..>2910)
                     /locus_tag="VFQ61_33390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959449.1"
                     /translation="VHATCRAQNTADLKFQHRQRGKVSQYPGRDRRRPWQIAAANASL
                     RLLSKPKDVDLDDSAWLETQSSPSAWRDASRRSERAGRPMPGSARASPLSQNHLRAAA
                     AFAAPSAQKRRQPGGSPKRLISGTP"
BASE COUNT          518 a          903 c          937 g          552 t
ORIGIN      
        1 cgtttccgcc ttcacgcccg atagactgcc gcttcgcctt caactcgtga agaacgggct
       61 cggcgcagcg gataccccaa acgtgcgcag gagttccaga gctttcgcct ccacctggta
      121 cacgtacgcg ggactattca ccgctcggcc gggctcgtcg gggttcgcga tgacgaggcc
      181 ggcggcgacg aactggtgca tggtctgcct ccgcaccgtc tctcgagtgt tcggcttgta
      241 tgcgtgcccg tactcgtccc tcacgaagtc catgatgggc gtgatcccca tgagcggggc
      301 tgccgctctc gaccaagcgg tgttcggctt cagcgccaag agcgcgagca acgtccaggc
      361 cgagcgatcg ttctgttgtt ccgacggcag gccgagggcg gccagaacct cacgagcttc
      421 tcttgttttt cgctggacag ggtcggccat cagcgtcgaa gccaatcaca tccgccacaa
      481 gttcgtcgag ctcctcttgc gcgcagcccg gatcctccaa gcgtttgccg agcgccagca
      541 attgatcggc cgcgggatac ctaaggttcc tgaggtcagt cgcgtttact tgggtgtgtc
      601 cgttgaattg gcgaaagtag cggtcagtca gcgtggagtt caagaacgcg gcgagccctc
      661 gggcgagcat cggcccaatg ccaccgccgt ttacgtggaa gacgttgagg tggttctcaa
      721 accccacctt cttccccgga acatcctcag gatcaaagac caccgccacg acgcgtcggc
      781 gctcctcctt cgcggagaag cgcttcacga gtacgtaggt gcctgatggc atccacagct
      841 cctcggtgct ggcatcgtcc agcaagccgt ttggtttctt gccttgcttg ggccaccgaa
      901 cgcggccctc ggcgaagtgc gtgggataga tcaaaggtcc gatgcctgga ccagggtccc
      961 cacgcagcgc ccactttgcg cgaaaatcga cgaccttccc cgtggagaca ccaagtccga
     1021 tgtcacgaag gttgcaagct agcgcggcga acgcagtcgc cacgtcctgc ccttccgcgt
     1081 tcggaacgag gtgtatgaag gattcgcgat ctcgtggatg tacgatctcg gcgaacggca
     1141 cctgtctcgc cttcggttcg tcgcgtgggc tcggactcga cgagaccgtg actaggcgct
     1201 gaggcgcacc cttcacggcg tggaagatca cgttttcttg gagcacctcg tcctcggaga
     1261 acgcagcggt tcgagagtca aaaacgtgaa tacgctggat agccatctct cgcagaagaa
     1321 actcgcgaaa cggcttgaag taggggccgt tgcagaagct tctcggggtg atggctacaa
     1381 gttcacctcc cggcgcgagg cgctggatcg cgagcgcgac gaacgccgtg tagatgttgc
     1441 tcgtttcgat gcccgcccga cgaaggagca gacggctggc ggatttcgag ttgagcttgc
     1501 ggtacggagg attgaggatc gcgtgcgtgt actgcgcggg cgtgcgacgg ctaaagagcc
     1561 ctcgatccac gtcttcaacg gtggcctcaa tgaagtcatg ctcaacgagc cgccccttca
     1621 gatctacgcc agcttcacga caggacgagg caagctcgtc cactgctctc cgaaggtatc
     1681 ccagcagaat gggatcgatt tcgaacccgg tcacctcaat gatgcgaggc cgctgctttc
     1741 gatttagcgc ggcgatggtt gccgcagtga gaagagcgcc cccgcccgcc ccagcatcca
     1801 gcagtcgaac ctcgtcaccg aagtcggtga agagggaggc catgaacgaa gcaattggtg
     1861 agggggtgag gaactgtcca aactcggagc gcttcgcggg gtccagccgg gccgacgctt
     1921 cccgtcgcca tgtgtccacc tggttgagga ggacggcggg cgtgaacgac atcacgggcg
     1981 ctggcgttcc catccccgcc ggttaccgtg ggtttctgcc gctgccaaga acaaaggtga
     2041 tcttttgttc aggtcgatcg agagcggcgg gccgcagcac tttccggggc cccgcgtgcg
     2101 caatcgccgc gaggcacgcg gccacgtcac cgaacgagcg cgggcgctcc ccgggaacgt
     2161 ggacgtggac ccgctgtggc cgaccgtaac cacgaccacg ctactcatgg gcaagcgtgc
     2221 gcggacctcg ctgaggcgag ggtcccacat gacgagatga gctcgccggg taccatcgtc
     2281 ttcatcggtg aggtcgcatt cggcaaccag aagttcactt cgtcgacccg cccgttcact
     2341 gcccgggtcg cgagaaagtc gaaccaagcc cgatcggtgt tggcgatgaa cggctcggtg
     2401 ttcgggtacc tcattcgatg tggcggcggg aggtgatcac agctggcaac gcctccggcg
     2461 caccgaccag gatcctgagg gtcctgtcga gtgtcaatgg cgcccgtcag gcgtttgtta
     2521 cttctcaggg agttcccgaa atcagccgct tggggctgcc cccaggttgc cgcctttttt
     2581 gcgcggaggg cgcggcgaac gcagcggccg cgcgcaggtg gttctggctc aggggtgaag
     2641 cgcgggctga acccggcatg gggcggcccg cacgctccga cctgcgcgaa gcgtcacgcc
     2701 acgcgctcgg cgagctttgc gtttcgagcc aggcgctgtc gtcgaggtcg acgtccttcg
     2761 gcttgctcaa gaggcggagc gaagcatttg ccgctgcgat ctgccacggg cgccgacgat
     2821 cgcgaccggg atattgggac acctttcccc gttgacggtg ctggaatttg agatccgcgg
     2881 tgttttgggc tcggcacgtg gcgtgcaccg
//
LOCUS       DASTKU010000944         9497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1526465, whole genome shotgun sequence.
ACCESSION   DASTKU010000944 DASTKU010000000
VERSION     DASTKU010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526465"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VFQ61_33395"
     CDS             <1..463
                     /locus_tag="VFQ61_33395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959450.1"
                     /translation="TTRSQAAKPLTMPGVWLPTTSASRQSLRRAAWVAGTLGLLGIGW
                     LLGSRSAGTGTSHAELAAHAELAAPAPPKSKGAPTFNTTSDRSAFTPTSDGTHPLPTA
                     SPAVTTAPAPAEPPATRPQRTSSAGGTSKPAQATPPPRRTRNERLSRDFGF"
     gene            467..1570
                     /locus_tag="VFQ61_33400"
     CDS             467..1570
                     /locus_tag="VFQ61_33400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959451.1"
                     /translation="MTLSGRRSEFIAPAWFTALVLCAGLGFAPIARAAEPDARTRAAA
                     RELAVQGLESYEANDFRTALERFDRAAALFAAPTITIMQARTLEKLGHWVEALDRYAV
                     TIAMSPPADAPDAFRRAIADAKIESQALRERTPTLELRVQRGDRNSGLPPETAGAEAG
                     LEVRLDGQLVPPALWNVEQLVDPGPHTVTLSGPTTRSFQRLVRLEPAERARLDLPTSG
                     PKSRKASESGAETAAVATPSADGSLRSNTASRDDHSRDPRKTWAFVAFGTGAVGASVF
                     AITGGLALSQRGKLQDVCRPGCPESASDDLSSFRLNRNLATASAALAVLGAGIGTYLL
                     VAGKPSQEHIALRLTPGWQSGAHSGAATLSAAF"
     gene            1820..3376
                     /locus_tag="VFQ61_33405"
     CDS             1820..3376
                     /locus_tag="VFQ61_33405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9959452.1"
                     /translation="MDDSNSAPTLRQLPNGRRAVAQLNIVKGPGLGHRFNVDAPLLMG
                     RSPEATIVIEHEEISRIHARVWNTDGSSYYVEDLSSRNGTFLNGIRIERAPLCLGDRI
                     RLGGEVELLFTSSDGLSDRLAERQRYETLGRLGVGAAHDLNQIVTAIMSSAAFLKETL
                     LKDNLGKEPARDVRYGRERVDPDVQDCISDLVLAASRADRFAHSMLEFARGGRSQRTL
                     LDLSALVGEALELLQPTLSSHILVQVRRARGVFVLGNSSELSQVLLSLGWNAVDAMPH
                     GGSLNIETSVVDFIPKEINLPGAKRAALLSVTDTGVGMTEETQRRLFEPFFTTKAQGQ
                     GYGLGLASAHEIVTLHGGIIRVQSALARGTRVEVYLPAPDPDSLRRTETIERPLDRAP
                     APSPGRLLLVDEAGIVRRMLARLLRRSGFEVFEAGSIDECLHRLARDSIELAIIDSKV
                     QHRSGPDTPARLASARPGLKLIFAANGTDGAALSDGQPRDDLAILQKPFAFEQLLMSI
                     ERLLGLPSRR"
     gene            4071..5807
                     /locus_tag="VFQ61_33410"
     CDS             4071..5807
                     /locus_tag="VFQ61_33410"
                     /inference="COORDINATES: protein motif:HMM:NF027224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR repeat-containing protein"
                     /protein_id="HET9959453.1"
                     /translation="MRSGYGALVGLLCFGVGCSSPESPSMATGGSNASGGRTTSGSQG
                     GQPGTATGGVTSSGGLAQGGAAGSFGAGAGQDATGGTAGASVSTAGRGGGSEGGGAGS
                     TSPGGTSNGGTTTAGGATDRGGTGGGSNAQGGSGTSGAGTSGQAGSGGQGGGVTAPIA
                     ETVPIAKVWSGHPVGFYLLTHKDRQFVAFYDENRALTVGVRKLGSRDWQLTKLATSVG
                     WDSHNDIVMAVDSTDILHVAGNMHSSALIYFRSKSALDPSTLERVTNMVGKDETACTY
                     PQFFLDSRGELVFMYRSGESGNGNHIFNSYDTQTKQWSRLLNSALTDGQGQRNAYPVG
                     PIQGPDGFWHLVWVWRETADARTNHDLSYARTQDLKVWQSAAGKTLNLPITLASSDIV
                     DAVPQNAGMINNNTKVGFDSMKRPIVAYHKFDAAGNTQLYNARFENGKWVSHLTSNWN
                     YRWDFGGNGTLVFEIEVQPVELQPDGTLTQSFYHAKFGGLGAFRLNENTLASEASIAP
                     PVPYPADLAKVESKEAGMEVRFRKDANASADPNLIYMLRWETLPSNRDQPRDPPYPAP
                     SELRLYAFPIKR"
     gene            complement(5797..6768)
                     /locus_tag="VFQ61_33415"
     CDS             complement(5797..6768)
                     /locus_tag="VFQ61_33415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET9959454.1"
                     /translation="MTKPLLRVADLAVSFASPEGPIRAVDGVDFELQPGETLALVGES
                     GSGKSTLARAVIGLTATSRGSIELEGEQVVGADRAKLRELRRKMQLVFQDPYESLNPR
                     MTIGRTIAEPLLYQANSNAAQRAGEVERLLARVGLDPGFAARYPHEFSGGQRQRISLA
                     RALAMRPRLLLCDEVTSALDVSVQAQILELLRTLQRELGLAYLFITHDLGVVRSIAQR
                     VAVMYLGKLVELRTTESFFEQPRHPYSQALLGSVPRLRRAGDAASSGLTGEIPSAARP
                     PSGCRFHTRCPKVFDRCPREEPGLYPIPNGVSRCFLDENEAGGEINV"
     gene            complement(6765..7823)
                     /locus_tag="VFQ61_33420"
     CDS             complement(6765..7823)
                     /locus_tag="VFQ61_33420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9959455.1"
                     /translation="MTETERSRAPEQGRALLQVNGLSVQFRTRERVVRAVEALDFHVD
                     QGEIVGLVGESGSGKSVTSLALMGLLPKPAGHLTAGSIQFAGRPISQLPAAELAALRG
                     DRLAMVFQDPLTSLNPYLRVEEQLVEVLMFHRRATRAAARKRAVQLLDRVGIPDAERR
                     IRAYPHQLSGGMRQRVCIAMALMCEPDLLIADEPTTMLDVTLQAQILGLLRELRRERE
                     MAILFITHDLGVVAELCDRVLVMYAGRIVEQATSEQLFRRPLHPYTEALLASTPRVDL
                     PGGGQLRAIGGLPPRLGAEPLTECAFADRCPRVRPACRDGEPALAPAPDARLRRCIAL
                     LEEMESPPSPQRALARPA"
     gene            complement(7823..8707)
                     /locus_tag="VFQ61_33425"
     CDS             complement(7823..8707)
                     /locus_tag="VFQ61_33425"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9959456.1"
                     /translation="MLPRLFSGPPSDSPAARAWRRLLQNRAAVSSGIVLTLMVLGCVA
                     LPALLGLDPNATAPALRHQPPSAAHWFGTDAVGRDLFARVLVGGRVSLSIGLAATLVS
                     VTIGVAYGAVAGFYGGKLDEILMRFVDFLYGLPYMFLVILIMLLFAESARGQVLPVFL
                     ALGLIQWLTMARIVRGQVMALRHRDFVLAAELSGASDVRILVHHMLPNILGVIVVYAT
                     LMVPSVIILESFLSFLGLGVALSWGQLVSAGVQVVNSVHSYWWLLAWPSLFLGITLLA
                     LNFLGDGLRDALDPKGRS"
     gene            complement(8707..>9497)
                     /locus_tag="VFQ61_33430"
     CDS             complement(8707..>9497)
                     /locus_tag="VFQ61_33430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9959457.1"
                     /translation="EAALRERYGVPKSGTAFFLVYVQRLLLAGTLGPSLKVQGRSVEE
                     LLLPAFPVSLSLGLLALLIALSIGILLGIRAGLKPNSWEDHLSMALSLVGVSLPNFVI
                     GSALMIAFALYFRWFPVAGWGGYRNLVLPALTLALPNAAVIARLTRSGMIEAMQQDYV
                     RTARAKGLPESWVVGRHALRAALLPVVSYLGPAGAAVMTGSFVVEVLFSVPGMGQWFV
                     KGAINRDYSVVLGTSIIYFALIISFNLLVDLIYALLDPRLRETR"
BASE COUNT         1870 a         3007 c         3031 g         1589 t
ORIGIN      
        1 cacgacgcgc tcgcaagcgg ctaagcctct cacgatgccg ggggtttggc tgccgaccac
       61 ctccgctagc cgtcagagcc tgcggcgcgc ggcgtgggtg gcgggcacgt tggggctgct
      121 tgggattgga tggctgctgg gctcgcgcag cgcgggaacg ggaacctcgc acgcagagct
      181 cgcggcccac gcggagctcg cagcgcccgc gccgcccaag agcaagggcg cgccaacttt
      241 caacaccact tcggatcgtt cggcgtttac gccgacgagc gacgggacgc atccgctccc
      301 aaccgctagc cccgccgtga ccacggctcc ggcgcccgcg gagccgcccg ctacgcgccc
      361 gcaacggacg tcgagcgcgg gaggcacgtc taagcctgcg caagctacac caccgccgcg
      421 ccgtacccgc aacgagcgcc taagtcgcga tttcggtttt tgagccatga cactctcggg
      481 ccgtcgatcc gaattcattg cgcctgcttg gttcaccgct ctggtcttgt gtgcgggttt
      541 gggattcgcg cccatcgccc gcgcagcaga gccggatgct cgaacccggg ccgcagcgcg
      601 cgagctggcc gtccagggcc tcgaatccta cgaagcaaac gacttccgca ccgctctaga
      661 gcgcttcgat cgcgcggccg cgttgttcgc cgcgccgacc atcacgatta tgcaagcgcg
      721 tacgctcgag aagctcgggc actgggtcga ggcgctcgat cgttatgcgg tgacgattgc
      781 catgagcccg cccgcggacg caccggatgc ctttcgacgc gccatcgcgg acgccaagat
      841 cgaatctcaa gccctgcgcg aacgcacgcc aacgttggaa cttcgagtgc aacgaggcga
      901 ccgcaactcg ggtcttccgc cggagaccgc cggggcagag gctggactcg aagtgaggct
      961 cgacgggcaa ctcgtgccac ccgcgctctg gaacgtcgaa cagttggtgg atccaggacc
     1021 acacaccgtg acgctttctg gcccgacgac acgtagcttc cagcgcctgg ttcgcttaga
     1081 gcccgcggag cgagcgcgct tggatttacc gacgagcggg ccgaaatcga gaaaggcctc
     1141 ggaatctggc gcggagaccg ctgcggtcgc gacgccgagc gcggacggca gcttacgttc
     1201 gaacaccgca tcgcgcgacg atcactctcg tgacccgcgc aaaacttggg ccttcgtcgc
     1261 attcgggacc ggagcggtgg gtgccagtgt attcgcgatc acgggagggc tcgccttgag
     1321 ccagcgcggg aaattgcagg acgtgtgtcg tccaggctgt cccgaaagcg cctccgacga
     1381 tctctcctcc ttccgactca atcgcaatct cgcaaccgcg tcggcggcac tcgcggtgtt
     1441 gggcgcgggc atcggaactt acttactcgt ggcgggcaaa ccttcgcagg aacacatagc
     1501 gttgaggctg accccaggct ggcagtcagg tgcccactcg ggtgcggcta cgctcagcgc
     1561 agcattctga gaacactgag ctgttctgcg cttttctgcg tccctgctcg ctcgctgagt
     1621 gtctccaagc accacgcccg gttcgatccg ttcgcccaat cgcatctttg gttcgagttc
     1681 atctgcagtg ccggtttccg atccgtaggc gcgagcgccg acatgtgcgt tcgactctaa
     1741 gcaacaccta cccaatgtgg cacgaatggc gttctgatct tcgttcccga ggaccaggga
     1801 cgtacactgc tgtgtcacga tggatgattc caactcggcc ccgaccctga ggcaattacc
     1861 caatgggcgc cgcgccgttg cgcagctcaa tatcgtgaaa gggccggggc tcgggcatcg
     1921 cttcaacgtg gacgcgcccc tgctgatggg gcgttcgccc gaagcgacca tcgttatcga
     1981 gcacgaagag atttcccgta tccacgcgcg ggtctggaac accgacggaa gctcgtatta
     2041 cgtcgaggat ttgagcagcc gcaacggcac gttcttgaac ggcatccgca tcgagcgcgc
     2101 gccgctttgc ttgggagatc gcattcgctt gggcggcgaa gtagagctct tgttcacgag
     2161 ctcggatggg ctgagtgacc gcctggcgga gcgacagcgc tacgaaaccc tcgggcggct
     2221 tggcgtgggt gcagcgcacg acttgaatca gatcgtcacg gccatcatgt ccagtgccgc
     2281 gttcctcaag gagacgctgc tcaaagacaa cttgggcaag gagcccgcgc gcgacgtacg
     2341 ctacggtcga gagcgggtcg atcccgacgt tcaggattgc atctccgatt tggtattggc
     2401 ggcctcgcga gcggaccgtt ttgcacattc gatgttggag ttcgcgcgcg gtgggcgttc
     2461 ccagcgcacg ctgttggact tgtccgcgct cgtgggagaa gcgctcgagc tgctgcaacc
     2521 caccctgagc tcgcacatct tggtgcaagt gcgacgcgca cgcggggtgt tcgtgctcgg
     2581 caattcgtcc gagctgagcc aggtactcct gagtttgggt tggaatgcgg tggacgcgat
     2641 gccgcatggc ggctcgctga acatcgagac cagcgtcgta gatttcatcc ccaaagagat
     2701 caaccttcct ggggcgaagc gcgcggcgtt actgagtgtc acggataccg gcgtcggaat
     2761 gacggaagag acgcaaaggc gcctgttcga gccatttttc acgaccaaag cgcagggcca
     2821 aggctatgga ctcggactcg cgagcgcaca cgaaatcgtg accctacacg gcggtatcat
     2881 ccgcgtgcag agcgcgctcg cgcgcggcac acgcgtggaa gtctatttgc cggcgccgga
     2941 tcccgatagt ctacgtcgta cggagaccat cgagcgcccc ctcgatcgcg cgcccgcgcc
     3001 gagcccgggt cgtctcttac tcgtggacga ggccggcatc gtgcggcgca tgctagcgcg
     3061 actcctgcgc cgctcggggt tcgaagtctt cgaagccggc agtatcgacg aatgcctgca
     3121 tcgacttgcg agagactcca tcgaactcgc catcatcgac tccaaagtcc aacaccgttc
     3181 aggcccggac acccccgctc gactggctag tgcgcgcccc gggctcaagc tcatcttcgc
     3241 cgccaacggc acggacggcg cggccctcag cgacggacag ccgcgcgatg accttgccat
     3301 tctccagaaa ccgttcgcgt tcgagcaact cttgatgagc atcgaacgcc tgctcggcct
     3361 cccctcccgg cgctgatgcc tcacccccga ttgcgctcga acgagactaa tctcccaccc
     3421 gccgcggcga tgggccgagc aacttcgtag ttctgcgcat ttcactctaa cgtgcgcgtg
     3481 ggaaacgtac ctgttttgca ctcgcgtgga cgcgcccacc ggggcggagc agtgacggag
     3541 ggggccggcg cggtatcgta tgggtttgga ggaaacttgg ctgcgcatgc gcggttaggg
     3601 acgagcttcg cgctctttac ggagtggccg acgagcaagc cccggagaga cgcaaggtca
     3661 gtggacaccg tgggatggca cagtcaccgt gccaatttcg gacctcccat ttctacggtc
     3721 acgacgaatc gcaatcttcg caacaaatca tttgttccag atcacaaccg atttacgact
     3781 cgagccgtcc acgaccaccc tgagattggt tgacgctttt tgctactcgg cttcacaccc
     3841 cgctgaatat gcatttggga cgaggcgcag ctcgaatcgc tgggtagatg gcgaagcgtt
     3901 caggggttcg tgaagggtgg gtgagggcat ggcgggccgc gattcgaacg aatttttggg
     3961 tgaaggcgcc gtatatcgag gcgagtgtaa agaaggtttc gtcatggcac gcgagcaggg
     4021 agtgtcgaag agggttgcag tggatgcgcc gaggcgataa gtgctgattc atgaggtctg
     4081 gatacggcgc gctagtcggg ctgctgtgct ttggggttgg atgcagctct ccggagtcac
     4141 cctcgatggc aacaggcggc agcaatgcga gtggcggtcg cacgacgtct ggttcgcaag
     4201 ggggtcagcc gggcactgcg acgggcggag tgacttcgag cggtggttta gcgcagggcg
     4261 gcgcagctgg gtcattcggg gctggagccg ggcaagacgc aaccggcggt accgcgggag
     4321 cgtccgtttc gaccgccggt agaggcggag gcagcgaagg aggcggcgca ggatctacga
     4381 gcccaggagg aacctcgaac ggcggcacca cgacggccgg aggagccacg gatcggggag
     4441 gcaccggcgg cggctcgaac gcgcagggtg gttcgggaac cagtggtgcc ggaaccagtg
     4501 ggcaagccgg ctccggtgga cagggcggtg gcgtcacggc tccgatcgcg gagacggtgc
     4561 cgatcgcgaa ggtttggtcg gggcacccgg ttggctttta cttactgacc cacaaggatc
     4621 ggcagttcgt cgccttctat gacgagaatc gcgcgttgac ggtgggcgtt cgaaagctcg
     4681 gaagccgtga ctggcagttg acgaagctcg ctacttcggt gggttgggat agccacaacg
     4741 acatcgtgat ggcggtagac agcacggata tcttgcacgt ggcgggcaat atgcacagct
     4801 cggcgctgat ttacttccgc agcaagagcg cgctcgatcc gagcacgctc gagcgcgtga
     4861 ccaatatggt cggcaaagac gagacggcct gcacgtatcc acagttcttc ctggatagcc
     4921 gcggagagtt ggtgttcatg taccgctcgg gcgagagcgg gaatggcaat cacatcttca
     4981 attcgtacga cacgcagacg aagcaatgga gtcgactgct caactccgcg ctgacggacg
     5041 ggcaaggcca gcgcaatgct tatccggttg ggccgattca agggccagac ggtttttggc
     5101 acttggtctg ggtgtggcga gaaaccgccg acgcacgcac gaaccacgac ctctcttatg
     5161 cgcgcacaca ggatttgaag gtgtggcaga gcgcggccgg caagactctg aatttgccaa
     5221 ttacgttagc cagcagcgat atcgtggacg cggtgccgca gaacgcgggc atgatcaaca
     5281 acaacaccaa ggttgggttc gactcgatga agcgtccgat cgtcgcgtac cacaagttcg
     5341 acgccgccgg gaatacgcag ctctacaatg cgcgtttcga gaacggcaag tgggtctctc
     5401 acttgacgtc gaattggaat taccgctggg acttcggcgg taatggcaca ctcgttttcg
     5461 agatcgaggt gcaaccggtg gaactccagc ccgatggcac gctgacgcag tcattttacc
     5521 acgcgaagtt cggtggcttg ggcgccttcc gcttgaacga gaacacgttg gcttcggagg
     5581 ccagcattgc gccgccggtc ccctaccctg cggatttggc caaggtcgag tccaaggagg
     5641 cgggcatgga ggtgcgcttc cggaaagacg cgaatgcgtc ggccgatccg aatctgattt
     5701 atatgctgcg ctgggagacg ctgccgtcca atcgcgatca gccgcgagat ccgccttatc
     5761 ccgcgccaag cgagctacgc ctgtacgcct tcccgatcaa acgttgatct ccccgccggc
     5821 ttcattctcg tcgaggaagc agcggctgac accattggga atgggataca gcccgggttc
     5881 ctcccgggga cagcgatcga agactttggg acagcgagtg tgaaaacggc agcctgatgg
     5941 cggccgagct gcggatggga tttcgcccgt gagcccggag gacgccgcgt ccccggcgcg
     6001 acgcagccgc ggcaccgagc cgagcaaagc ttggctatag gggtggcgcg gctgttcgaa
     6061 gaagctctcc gtggtgcgta gttcgacgag cttgccgagg tacatcacgg cgacgcgctg
     6121 cgcgatgctg cgcaccacgc ccaagtcgtg agtaatgaac aagtaggcca gcccgagctc
     6181 gcgctgcagg gtcctcaata gctccaggat ctgcgcttgg accgagacgt ccagggcgga
     6241 cgttacctcg tcgcaaagca gcagacgcgg ccgcatcgcg agcgcgcgcg ccaagctgat
     6301 gcgttgccgc tgtcccccgg agaactcgtg cgggtagcgc gcggcgaacc cgggatctag
     6361 tcccacgcgg gcgagcaaac gctcgacttc tccggcgcgc tgcgcggcat tcgagttggc
     6421 ttggtacaac aagggctcgg cgatggtccg cccaatggtc atgcgcggat tcaagctctc
     6481 gtacggatct tgaaagacga gctgcatctt acggcgcagc tcgcgaagct tcgctcggtc
     6541 ggcgccgacc acttgttcac cttcgagctc gatgcttccg cgcgaagtcg ccgtaagccc
     6601 aatcacggcc cgcgccaagg tcgatttgcc cgaacccgat tcacccacga gggccagcgt
     6661 ttcccccggc tggagctcga aatccacgcc gtccacggcg cgaatcgggc cctcggggct
     6721 cgcgaatgag acggccaaat ccgcaaccct cagcaatggc ttggtcatgc tggcctcgcg
     6781 agagcgcgct gtggactcgg aggggactcc atctcttcga ggagggcaat gcaacgtcgg
     6841 agccgcgcgt cgggcgctgg cgcgagtgcc ggctccccat cacgacatgc tgggcgcacc
     6901 cgcggacagc gatccgcgaa ggcacactcg gtgagcggct ccgccccgag acgcgggggt
     6961 aatccgccga tggcgcggag ctgtccgccc cccggcaagt ccacccgtgg ggtgctggcg
     7021 agcagagcct ccgtatacgg atgcaacggc cggcgaaaga gctgctcact cgtggcttgt
     7081 tcgacgatgc gccccgcata catcacgaga actcgatcac agagctcggc cactacgcca
     7141 agatcgtgag tgatgaacaa aatggccatt tcgcgctcgc ggcgcagctc acgcaagagc
     7201 cccaagattt gggcctgtaa tgtgacgtcg agcatcgtcg tcggctcgtc cgcgatcaac
     7261 agatccggct cgcacatcaa ggccatggcg atgcagacgc gctgccgcat cccccccgat
     7321 agttgatgcg gataggctcg aatccgccgc tcagcgtcgg ggatgcccac gcgatcgagc
     7381 aactgaacgg cgcgttttcg agcggccgcg cgcgtggcac ggcggtggaa catcaagacc
     7441 tcgaccagct gctcttcgac cctcagataa ggattgaggc tagtcaaagg atcttgaaag
     7501 accatcgcca ggcggtcgcc gcggagggcc gcgagttccg ccgctgggag ttgactgatc
     7561 gggcgccccg cgaactggat cgagcccgcc gtcagatgac ctgcgggttt gggtagcagg
     7621 cccatcagcg ccaaactcgt cacgctcttg ccagacccgg attcgcccac caagccgacg
     7681 atttcacctt gatccacgtg aaaatctagg gcttcgaccg cccgcaccac gcgctctcgc
     7741 gtgcggaact gcacgctcag cccgttcacc tggagtaggg cgcggccctg ctcgggagcc
     7801 cgagagcgtt ctgtttcggt cattagctcc gtcccttcgg gtcgagtgca tccctcaagc
     7861 catcacccag gaaattcagc gccaacaagg taatgccgag gaaaagactg ggccatgcca
     7921 acagccacca atacgaatgc accgagttga ccacttgaac gccggccgag accaattgtc
     7981 cccaggagag cgccaccccg agacccaaga acgacaggaa cgattcgagg atgatcacac
     8041 tcggcaccat caacgtggca tacacgacga tgacgccgag aatgttcggc aacatgtggt
     8101 gcacgagaat gcgtacatca ctcgcgccgc tcagctccgc ggcgagcacg aagtcccggt
     8161 gccgaagtgc catgacctga ccccgaacga tgcgggccat ggtcagccac tgaatcaagc
     8221 cgagtgccag aaacaccggg aggacttggc cgcgagcact ctcggcgaac agcagcatga
     8281 tcaagatgac caagaacatg tacgggaggc cgtacaaaaa atcgacgaag cgcatcagga
     8341 tctcgtccag cttacccccg taaaagccag ctacggctcc gtaagcaaca ccgatggtca
     8401 ccgaaaccaa cgtggccgcg agcccgatac tgagcgagac gcggcccccc accagaacac
     8461 gcgcgaacaa gtcacggccc accgcgtcgg tgccgaacca atgcgcggcg gagggtggtt
     8521 ggtgacgcaa cgccggcgcg gtcgcgttcg ggtccaagcc gagcagcgcc ggcaaggcca
     8581 cgcagccgag caccatcaac gtgagcacga tcccactcga caccgctgcg cgattttgga
     8641 gcaagcggcg ccaggcccgc gcggcggggc tgtccgaagg cggcccgctg aacagacgcg
     8701 gtaacatcac cgcgtctccc gcagccgcgg atcgagcagc gcatagatta gatccacgag
     8761 cagattgaac gagataatca gcgcgaagta gatgatggat gtgccgagca cgaccgagta
     8821 atcgcggtta atcgctccct tcacgaacca ctgacccatg cccggcacgg agaacagaac
     8881 ctccaccacg aacgatcccg tcatcaccgc cgcccccgcg gggccgaggt acgagaccac
     8941 cggcaggaga gccgcccgca gagcgtggcg cccgacgacc caagactccg gcagcccctt
     9001 ggcgcgcgcg gtacgcacgt aatcctgctg catggcttcg atcatcccgc tgcgcgtcag
     9061 gcgggcgatc acggccgcgt taggcagcgc gagcgtgagc gcgggcaaca ccaggttgcg
     9121 gtaaccgccc caacccgcga ccggaaacca gcggaagtag agcgcgaacg cgatcatcag
     9181 cgccgagccg atcacgaaat tcggcagcga gacgccgacg agcgagagcg ccatactcag
     9241 gtgatcctcc caggagttcg gcttgagccc agcgcggatc ccgagcaaga tcccgatgct
     9301 caatgcgatc agtaaggcca gcaaaccgag cgagaggctc accggaaaag cgggcaacaa
     9361 cagctcctcg acgctccgcc cctggacctt caaggagggc cccagggtgc ccgcgagcag
     9421 caagcgctgg acgtagacga gaaagaacgc ggtgcctgac ttcggcaccc cataacgttc
     9481 gcgcaacgcc gcctcca
//
LOCUS       DASTKU010000945         5319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_630648, whole genome shotgun sequence.
ACCESSION   DASTKU010000945 DASTKU010000000
VERSION     DASTKU010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630648"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="VFQ61_33435"
     CDS             complement(<1..1189)
                     /locus_tag="VFQ61_33435"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9959458.1"
                     /translation="MADRAPEVPEALAGPSQQEPAGDGATHSEYHASEAENSHTRQPC
                     VDTQRSAESDLADNGQERTFPSQAPDERHTLRSPGVSEPRGCLNEDDALEFAQGLLSA
                     SEIERAEAHVDSCASCRRLLGEAMLAVDPLAATEDGSTPEFALLRPGNVLGARYRILR
                     FHASGGMGEVYEAYDVELGERVALKTISPAAGDRERAVRYLKAEVQLARRVSHPNVCR
                     TFEFGTDDNPMTGAKLHYLTMEFVEGESLCKYLRKHGPLPLAQARQIAEQILRGLGAA
                     HQAGVLHRDLKTDNVLLRSGPKGSHALLSDFGLARALDFEGYGARQSAQGWVGTPDYM
                     APEQLEGRPLTAASDLYAFGLVFYEMLTGKLPFPRCRHTVALSARLKVTPSAPSRENP
                     EIPP"
     gene            complement(1182..2147)
                     /locus_tag="VFQ61_33440"
     CDS             complement(1182..2147)
                     /locus_tag="VFQ61_33440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9959459.1"
                     /translation="MSLEGSSAESTLQAMYLDGRRKWSDVDMGFPGFADHCERVLGMA
                     KADTSSDTLQVGSRPESGVLDGDLQFRRRASLRTDGARPDQLLDRIAVNGADIYLSCA
                     CAQGNSRARQHLEAQSSVLVRSAISRVRRDPEFVRETTQFFWERLLGGRSPRIGQYAG
                     RGPLLAWLRVVATRTALDRLRESPADADALRNTVLFEPAHSAESELTRSRYAAAFQRA
                     LTDAVSKLTRRERNVLYMHLRGPCSLDQIARVYNVHRSTVSRWLDGARSQVFTTVHQV
                     LTRECGLSESEFDNAARLIRSELDIIVPTASLSADAERESRGQDG"
     gene            complement(2240..2776)
                     /locus_tag="VFQ61_33445"
     CDS             complement(2240..2776)
                     /locus_tag="VFQ61_33445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959460.1"
                     /translation="MPSTSDIDMMDNTHDSSAGSLTVDLSSPAGALRSLAISPTLPAG
                     GPESAADGLLFDDAASIRITQARFTKLKNLGFNVTGTVEYDSTNGAVTGFTFPEAHRR
                     FALRREPSIKSLARIVYFQTERMECLLERVGELADRLDQCVVVDGKKGNGKEREDGAL
                     TLAGERPANKDGPIIAPQ"
     gene            complement(2813..>5319)
                     /locus_tag="VFQ61_33450"
     CDS             complement(2813..>5319)
                     /locus_tag="VFQ61_33450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959461.1"
                     /translation="GAPNDTTPEAGASQSEPTRGGSKSTGSSASTGMTHGGSMGGQVV
                     VAGESGGSAGDVMGGRSAGGMGSTSAGVAENGGTVTDAGGGGTGAIDCNGVASQPVIK
                     VSPATQYCAGTPVMIDVAAEGGPEPFTWNLQSAGLAIDPPEGARSSITGTVEGGGTRV
                     LQIGVKNGCGNITEQQVTLAERQPPSLTSTSLPLACTGFLYGIDPVRDLGMTEGDAVE
                     LSGSSWLHVENGRIYGTPSASTIETIHVSSRATACPSAELDLTVQSVSCDVFPVRGTA
                     PRAFPQPCAGQPYQVTMDFRAKVRWSLPAFLDPGLTIDAAGASTRTVTVHGTPTVQGP
                     LLLDAKNANNQSYRFGYALKPRNACYFAYVVDSNGTLVVLDPVLSSSSQPQIRPLPEQ
                     LGVGNAARVVDFAFAPDGSVLLYHLQDSAGSSTLAALDLRSLQDTSWPVPGQLVAYAW
                     AEDARRIAVLSQTSAGTLLGGLTIVPGSPPTITALESTTAPREALSLTWFAGNRVVLR
                     TQSDSLYDALWQAARFENYRAVGGQYSPGASLAATASGLFVLKEQQRSFDFISAMPKF
                     LSASYSVLSPSRALFLDAQSPVGGTVAVYGLVAQRAEQYTALQECDRVVAWAKDRERV
                     ACLRESTAVSGLRTFDLREGDSGSGQAIATATAPGTPPTFTTLAGSRRAWSASGKWFA
                     YQTDGGLHIAQGDEPRVVYYDATVSATPAQVALSFSPTEDVLLEATESKISAILLTPP
                     VLSEATPAAKANRNLQLQARSCVEDFWQDPSAWCGAWGDEPTRIRWYAEAPIAAMRAS
                     DGTLYVVDFGRANLGGTDEVTSSREANGSWEFQPAR"
BASE COUNT          956 a         1709 c         1596 g         1058 t
ORIGIN      
        1 tgggggggat ctcagggttc tcccgactcg gagcgctcgg cgtcactttc aatcgtgcac
       61 tcagcgccac ggtgtgccgg cacctcggaa atggcagctt gccggtcagc atttcgtaaa
      121 acaccagccc gaaagcgtac aggtcgctcg cggccgtgag cggcctgcct tcgagttgtt
      181 ccggagccat gtaatctggc gtcccgaccc agccttgtgc gctctgccgt gctccgtagc
      241 cttcgaagtc cagcgcgcgc gccaggccaa agtccgacag caatgcatgc gaacccttgg
      301 ggcccgacct gagcagcacg ttgtccgtct tcaaatctcg gtgcaacact ccggcttggt
      361 gggcggcccc caacccgcgc aggatctgct cggcaatttg gcgtgcttga gccagcggca
      421 atgggccgtg tttgcgaaga tatttgcaca ggctctctcc ctccacgaac tccatcgtga
      481 ggtagtgaag cttggccccg gtcatggggt tatcgtcggt cccgaactcg aaagtgcggc
      541 acacgttggg gtggctcacg cgtcgcgcca actgcacctc tgctttgagg tagcgaaccg
      601 ccctttcccg atctccggcc gctggcgaaa ttgtcttgag ggcgacccgc tcacccaact
      661 ctacgtcata ggcctcgtac acctcaccca tgccgccgga ggcgtgaaat cgcagaattc
      721 gataccgcgc accgagcaca ttccccggcc tcagcagcgc aaattcgggg gtggagccgt
      781 cctcggtagc cgccagcgga tcgacggcaa gcatggcttc accgagcaag cgccgacaac
      841 tcgcgcagct gtcgacatgg gcttcggcgc gctcgatctc acttgccgag agcaagcctt
      901 gggcaaactc gagcgcatcg tcctcgttga gacaccctct gggctccgaa acaccaggac
      961 tgcgcagggt atgacgctcg tccggtgcct ggctcgggaa tgtccgctcc tgtccgttat
     1021 ccgccaggtc cgattctgca ctgcgttgcg tgtcgacaca gggctgccgg gtgtgggagt
     1081 tctcggcttc ggaggcatgg tactcgctat gcgtggcgcc atctcccgcc ggctcttgct
     1141 gagagggacc ggcgagtgcc tcgggaacct cgggtgctcg atcagccatc ttgcccccgc
     1201 gattcgcgtt ctgcgtcagc tgacagcgac gccgtaggaa cgatgatatc gagttccgat
     1261 cggatgagcc gcgccgcgtt atcgaactcg ctttcgctca agccgcactc acgcgtcaaa
     1321 acctggtgca ccgtggtgaa gacttggctg cgtgctccgt ccaaccaacg cgagaccgtc
     1381 gagcgatgga cgttatacac tcgagcgatc tggtcgagac tgcaggggcc acgcaaatgc
     1441 atgtagagca cattccgctc gcgccgagtc aacttcgaga ccgcgtccgt gagtgcacgt
     1501 tgaaatgcgg ctgcgtaacg tgagcgagtc agctcggatt ctgcgctgtg ggcaggctcg
     1561 aagagcactg tattcctgag tgcgtcggca tccgccgggc tctcgcgcag tcgatctaga
     1621 gccgtgcgcg tcgccaccac ccgcaaccaa gccaacagcg gtccgcgccc tgcgtattgt
     1681 ccgatgcgcg gcgagcgccc ccccagtagc cgctcccaaa aaaactgcgt cgtctctcgc
     1741 acgaactccg ggtctcgccg tacccgagag atcgcgctgc gcaccagtac actggattgt
     1801 gcctcgaggt gctgacgcgc acgcgagttt ccctgggcgc aagcgcaact caggtatatg
     1861 tcggctccat tcactgcgat tcgatccagt agctgatcgg gtcgagcccc gtcggttcgc
     1921 aacgaggctc gacgccgaaa ctgcagatct ccgtctaaca cccctgattc aggcctactg
     1981 ccaacctgaa gcgtatcaga gctggtgtcg gctttcgcca tccccaaaac ccgctcacaa
     2041 tgatccgcga accctggaaa ccccatgtcc acgtccgacc acttgcgacg tccatccagg
     2101 tacatcgcct gaagcgtcga ctcggcactg ctaccctcga gtgacattct actactctag
     2161 ctcatttcaa cccgcgcgct gtttgccctc cctcttcggc gccctctggt atgcgattcg
     2221 tcgcgagcgg gactcgcgat cattggggtg caattattgg cccgtccttg ttggcgggcc
     2281 tttcgccggc caaggtcaga gccccgtcct cccgctcttt tccattccct tttttaccgt
     2341 ccacgacgac gcactgatct aggcgatcgg cgagctcccc gacgcgctcc agcaggcatt
     2401 ccatgcgttc ggtttggaaa tagacgattc tggcgaggct cttgatgctc ggctctcgac
     2461 gcaacgcaaa gcgccgatgc gcttccggaa aggtgaagcc ggtcacggca ccgttagttg
     2521 aatcgtactc gacggtgcca gtcacgttga acccgaggtt cttgagtttc gtgaaccggg
     2581 cttgcgtaat ccggatgcta gccgcgtcat cgaacaacag tccgtctgcc gcggactcag
     2641 gtccgcccgc gggcagagtg ggagagatcg ccaaagagcg cagggcgccc gcgggtgaac
     2701 tgaggtctac agtcagtgaa cctgcggatg aatcatgggt attgtccatc atatcgatgt
     2761 ctgaagtgct aggcattgct cgattccttc cgtttcgaat gtgctgtatc ggttagcgcg
     2821 cgggctggaa ctcccacgag ccattggcct ctcgagatga cgtcacctca tcggtgccgc
     2881 ccaagttggc ccttccaaag tccaccacgt agagcgtgcc gtccgaggcg cgcattgcgg
     2941 caatcggagc ctctgcgtac caacgaatgc gagttggttc gtctccccaa gccccgcacc
     3001 aggcgctcgg atcttgccag aagtcctcta cgcaacttcg cgcctgaagc tgaaggttcc
     3061 gattcgcttt ggctgcaggc gtggcttcgc tcagcacggg cggtgtcaaa agaattgctg
     3121 aaatcttcga ctctgtggcc tccaggagca cgtcttcggt tggggagaac gagagtgcga
     3181 cttgggcggg agtcgcgctg accgtagcgt cgtagtagac cacgcggggt tcatcgcctt
     3241 gggcgatgtg aagcccacca tcggtttgat acgcgaacca cttgccggaa gccgaccaag
     3301 ctcgccggct gccagcaagg gtggtgaatg tgggtggggt tccgggagcg gtcgcggttg
     3361 caatggcttg gcctgagccg ctgtcgccct ctcgtaggtc gaacgtccgc aagcccgaaa
     3421 cagcagtgga ctcacgcagg caggctaccc gctcccgatc tttggcccac gcgacgacgc
     3481 ggtcgcactc ttgcagcgcg gtgtattgct cggcgcgctg agccactagt ccgtacacgg
     3541 cgacggttcc gccaaccggg ctctgcgcat ccaagaagag ggcccgactc ggtgagagta
     3601 ccgagtagct agcgctcaga aatttcggca tggccgagat gaagtcgaag ctccgttgtt
     3661 gctccttcaa tacgaataat ccgctcgccg tcgccgcgag gctcgcgcca gggctgtact
     3721 gccccccgac tgcgcgatag ttctcgaacc gcgcggcttg ccaaagcgcg tcgtagagag
     3781 agtcgctctg ggtccggagt acgacccggt tccccgcaaa ccaagtcaaa ctcagtgctt
     3841 ctcggggtgc ggtcgtcgac tcgagcgccg tgatggtggg aggtgagcct ggcacaatcg
     3901 tcaaaccgcc gagaagcgtt ccggcgctgg tttggctcag cacggcaatg cgacgcgcat
     3961 cctcggccca agcgtaggcc accagctggc caggaactgg ccatgatgtg tcttgaagac
     4021 tccgcaggtc caaagcggcc agcgtggagc tgccggccga gtcttgcagg tgatagagca
     4081 gaacgctccc gtccggcgca aatgcgaagt ctacgaccct cgctgcgttg cccacgccca
     4141 actgctccgg cagcgggcgg atctgtggtt gcgagctcga gctcaagacc ggatccagga
     4201 cgaccagggt accgttcgaa tccacgacgt aggcgaagta gcaggcgttt cgcggcttca
     4261 acgcgtaacc gaagcgatag gactggttat tggcgttctt tgcgtccaga agcaacgggc
     4321 cctgaacggt gggtgtgccg tgcacggtca ccgttctcgt cgaggcgccc gcggcgtcga
     4381 tcgtcaagcc aggatcgagg aaggcgggca ggctccagcg taccttggcc cgaaaatcca
     4441 tcgtcacttg gtacggttgt ccagcgcagg gctgagggaa cgcacgggga gccgtgcccc
     4501 gaacagggaa cacgtcacaa ctcaccgatt gcaccgtcag gtctagttcc gcagacggac
     4561 aagcggttgc gcgcgagctg acatggatgg tctcgattgt actcgcgctc ggcgttccgt
     4621 agattctccc gttctccaca tgaagccaac tggatccgga gagctccacg gcgtcgcctt
     4681 ccgtcattcc caaatcacgg acgggatcga tgccatatag aaaaccggta caggccagcg
     4741 gcagagaggt actggttaga ctgggtggtt gacgctcggc gagagtcact tgctgttccg
     4801 tgatgttccc gcatccgttc ttcactccta tttggagtac acgagtcccg ccaccctcca
     4861 ccgtcccggt gatggagctg cgcgcgccct cgggtggatc tatcgcgagc cctgcgctct
     4921 gcaggttcca agtaaagggc tctggcccac cttctgctgc gacgtcaatc atgaccggcg
     4981 ttcccgcaca gtattgcgtg gcgggggaga ctttaatcac gggctggctg gcaacaccat
     5041 tgcagtcgat ggcaccggtc ccgcctcctc cagcatctgt gacggtgccg ccgttttcag
     5101 cgactccggc agatgttgat cccatgcctc ccgcgcttcg cccgcccatg acgtctcctg
     5161 cgctacctcc ggactcgcct gcaaccacaa cttgtccgcc catcgagccg ccatgggtca
     5221 tcccggttga cgcggagctt cccgtcgact tcgagccgcc ccgtgtcggt tcggattgag
     5281 aggcgcccgc ttcgggtgtc gtatcgttcg gtgctccgc
//
LOCUS       DASTKU010000946        14963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_185267, whole genome shotgun sequence.
ACCESSION   DASTKU010000946 DASTKU010000000
VERSION     DASTKU010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14963
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185267"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFQ61_33455"
     CDS             <1..217
                     /locus_tag="VFQ61_33455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959462.1"
                     /translation="TVHYDRAILDQGDSCPSPTNAGCAGCGECGAGLTCMAEERDAGT
                     CASCATDSDCCSPLVCDNGRCRALLTE"
     gene            827..1426
                     /locus_tag="VFQ61_33460"
     CDS             827..1426
                     /locus_tag="VFQ61_33460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9959463.1"
                     /translation="MQPATEAQLQKDVALMRRAALGDPAARREAVVRVLPRARRLCQS
                     LLGSASDSEDASQMGIVEVLRSAAHFRGECPLEHWASRIVARTALRMAKDERRKARMS
                     AAFLAEGEEDAVEAEYGPSRILATECLKKLPEVQRTALLLRCGFEYSIEEIAELTQVS
                     RNTVKDRLLRARESLRDLLRQNAESDQDPTIPGSARLIR"
     gene            complement(1498..3153)
                     /locus_tag="VFQ61_33465"
     CDS             complement(1498..3153)
                     /locus_tag="VFQ61_33465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET9959464.1"
                     /translation="MNSPLTDLLKRALMVGALEVKLVPGRRTIVVLAQGESEVKGDTQ
                     TPERILDLLSPVMTAEARRGLASGWAEWEFRLENRGPVRACVEMKMGLPHVSLFLDRC
                     DEGRSEDARAISRGREDAPREDLGPRPVPREVPVARAVVTSPEFAVSVPARRPPDETV
                     RLEPTRPLATQPPLPSVDQLYDDGMSGGSTAEIDELLGKMLELKASDLHVSSGAPPML
                     RVDGEMVPVRGYGPLAPQAIQRMLLPIVPPRNREEFTRSHDSDFAYELPGRARFRANI
                     FVDLRGMGAVLRVIPSKILSAQDLNLPKELLSLCHLPKGLVLVTGPTGSGKSTTLAAL
                     IDYINSNRSQHIITIEDPVEFVHPNKKCLINQRQVGEHTDSFKRALRAALREDPDIVL
                     LGEMRDLETIAIALETAETGHLVFGTLHTSSAPATVDRIIDQYPAEQQNQIRVMLANS
                     LKGVICQMLCKKQGGGRVAALEVMFGIPAISNLIRESKIFQIPTVMQTARKVGMCLMN
                     DSLIRFVKDGVVAPEEALSKSNDKAGLQLLFQQANIRVPELAG"
     gene            complement(3269..3868)
                     /locus_tag="VFQ61_33470"
     CDS             complement(3269..3868)
                     /locus_tag="VFQ61_33470"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET9959465.1"
                     /translation="MTRELEPLEVLILNAGLAQERGNSQVLCEFVQQELRGKGVACAV
                     ANVASIGPAQIAAARALVFVTGTYWSSWSSVLQAFLERMTETEGTSVWLGKPVVVLVS
                     AHQVGAFSVLCRLQGVLCALGCSVPPMTGVVITRVGERVRLNHPDEATDVWGLPDVRI
                     ALHNLLEAVRGSRDFRAWNSDREHFEAVWLQADPETGSG"
     gene            complement(3979..4818)
                     /locus_tag="VFQ61_33475"
     CDS             complement(3979..4818)
                     /locus_tag="VFQ61_33475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959466.1"
                     /translation="MAGWTRYGQSLRSDLRVNAAQIEGRGLPNSGQLLRELVATYSPT
                     PIGDPDGPPTSRHLANDSREPQAPKCERGLSVGPDGLTSQQREHFGERLDDAESETVA
                     NQARYERYVCRAQNAGRTPLSATDWVRADQRLRENADRGGHEESSVLDALGIAGTGGE
                     DVSLPSRGGGRVQVDAVTNSAVVEVRSVPDTPDPEGGPRVVHDSEALREARVAAKDSD
                     KVLVVVLTNADSRNVRPSRPLSAETVVLHRDSSSGEFSKWDPFTESWQPISVDAARRW
                     VSN"
     gene            5492..6106
                     /locus_tag="VFQ61_33480"
     CDS             5492..6106
                     /locus_tag="VFQ61_33480"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET9959467.1"
                     /translation="MRLVPGLRAPSFVIKALTGQTYTLEQLRGELVWLAFFRFATCPL
                     CNLRVHQLVDEWERTFAGKIHLFAIFQSPKERFTNYVSKYEPPFELVSDVELALYQQF
                     GVETRISATLHPKVATTLLEARRADIPLFDGPKDGGSFRVPADFLIDRNGVLRVCRYG
                     EHLADSIASPDVLTFIGEHAQTSVNPGSLPPPAPTTTPPQSLKT"
     gene            7073..8539
                     /locus_tag="VFQ61_33485"
     CDS             7073..8539
                     /locus_tag="VFQ61_33485"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HET9959468.1"
                     /translation="MSTRLRIGIVGLAIGVSALLSMAPSAAAADSNPPLDFDHGNAAI
                     EKVIPSVVPVIYEIVAPSDASIVLRITTIASNAWFDAIAPYHPTAVGVCSKLGRRPAA
                     ERTQRNKNVAILYASYQVLNSLLPNYKARWQALLRGEGLDPNDTREDDSAVGIGNRAG
                     KAVAKFREHDGMNQLGDEGGRKYNRMPYADYTGYEPVNTAFDLRDPSRWQPNLVTRGT
                     GLFQVQHFVTPQWAFTRPYSYSNPNRYRSPAPTASWVRNYASYRRQAEEVLAASAHLT
                     DEQKMTAELFNNKIESLGYSALYASEAFNFDLDRFVQYDFLTNAAAFDGGIATWNEKY
                     RWDTVRPFSAIRYLYRKQEVTAWGGPGRGTVTDMPGVEWQSYLPTADHPEYPSGSACF
                     CAAHAQASRRFLGADNFGWSVPFLKGSSKIEPGFTPARDVVISFETYSDFEKQCGQSR
                     LWGGVHFQSAIDAGRALCRQFGDLTYEFVAAHIAGKTR"
     gene            8718..10277
                     /locus_tag="VFQ61_33490"
     CDS             8718..10277
                     /locus_tag="VFQ61_33490"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET9959469.1"
                     /translation="MADPTRHTGEAGGPSAVGHGSVRPYSYAWRASLAAALFFGFYAL
                     ALSLAGGALYLAYASISSAHPVFVQSALGCALFAFAVLKATFFVKRPSFEAPGPEITA
                     SEEPKLFELIQSVCAQMGTRMPTRIYLIPDVNAFVAEVGGFMGLGSQRVMAIGVGLLD
                     VDDLSELRATIAHEFGHYVAGDTRLGSFIYRTRAAIAQVLTSLGGSWFSKPFEWYGAL
                     FMRVTQAVSRAQELAADRSSVTLAGRDALVSSLRHESRAAVLFQHFLGSELTPLLEAL
                     HRPDNLYEGFRVFWHARVEQGSVAELDVALENAGTTVYDTHPALGERVRFAESLSDPG
                     LARDRTPARSLLRDPEATERRMTDHILASTGNGPLTRVSWHEVTAKVYAPKLSGDAHL
                     TAGEVAHALGGAPDVSGALMTLLLNLRGIGVKTVGERLEPRLSEVPAQTRDDVSMRLL
                     TFKLGVLFGQALVEAGGEWRSDVGHPHRVLFRSEPLKPFELARAALLSRDGLVELERR
                     YWDARRRDPIA"
     gene            10420..11934
                     /locus_tag="VFQ61_33495"
     CDS             10420..11934
                     /locus_tag="VFQ61_33495"
                     /inference="COORDINATES: protein motif:HMM:NF012341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959470.1"
                     /translation="MAKETIVSDFKLKGREILSSYANFTIEGEMSAKVRRGEETFTIQ
                     ERNGIPKGTSDGENEAVYIPNDKAIESISKNIAPLLPAQVVIRSPKDILDAAAAFDLE
                     LVKRAGHNKRDWGGNACLAASTAFLQTLLKASGYEAYEVFLTEQSKTFYFPNIAFNMV
                     CGGEHVPGTKQDIQEMFFFAMKAKSVKEVFEIGTKFFRQFEKNLVRDGLPTGTAKERG
                     FVFPVKDNFEGLTRIKECAGQLGLKPSDYAIGTDNAFSEVQKGEELVKKGMYDMRFSG
                     TGLKTREQLVDFDGTVVNSAENFATMEDPGSEHDVEAHKLMNARFGDRVQIVLDDLVV
                     TQLRFIIPYLCGKKREDRAGNSVLIKLNQSGTFTETRLAVEVVLGLADAKQVEQFLSA
                     RGDLDFILSECRKVGVQSLAEALENIKSNGFTAFISHRSTEGSSEFLPYLPLMYGAVT
                     RRLWFKAGAPNGERNLQNYNPLIRAEEQLRARGITTEVAGFAGLPAGVKVPAAQ"
     gene            complement(11994..13010)
                     /gene="ylqF"
                     /locus_tag="VFQ61_33500"
     CDS             complement(11994..13010)
                     /gene="ylqF"
                     /locus_tag="VFQ61_33500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HET9959471.1"
                     /translation="MSMQWFPGHMKQARRAMAEALPSNDVVIEVLDARMPISSSNPVL
                     SKLRAQKPCIKLLGKTDLADPEITRAWVDHFASQAESQVVALLFSKDRAGEARARITE
                     LCRKLAPKRVQSARPVRVMVVGIPNVGKSTLINLLMERKVAKVSDQPGVTRAPQLVTL
                     KTGLTLLDNAGILWPRIDTESVLRLSLGGALPEAEMDYETVAAFAAQFLSKHYPKRLA
                     ARLAIPELPSSSELVLEAIARKYGCLRKGGQFDWRRASEILVHEFRSGVFGRVSLETP
                     ADLAARELTAIGEAAMADATSPEVRDNEEPSEAPPTGFASTAHSVVTHDRVSDPKDED
                     PTPD"
     gene            13249..13890
                     /locus_tag="VFQ61_33505"
     CDS             13249..13890
                     /locus_tag="VFQ61_33505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET9959472.1"
                     /translation="MRSSIFVLSLLALSACNNDPTKGKPQATVSSPLVTSTAAPAGGT
                     PAPAGVTTQYAFSNEGSQLAFVGAKVTGKHDGSFKAFRGTISVVDNDPAKSTVTVEAD
                     SASLDTDTAKLTGHLKSPDFFDVEKYPKVKFNSTSIVKAGSGYTVTGNLDLHGVTKSI
                     SFPASLQVAGDTATADAEFGINRKDFNIVYAGKPDDLIKDEVLIKLKLVAKKQ"
     gene            complement(13865..>14963)
                     /locus_tag="VFQ61_33510"
     CDS             complement(13865..>14963)
                     /locus_tag="VFQ61_33510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9959473.1"
                     /translation="EYLPLLPRLGSPLGAILERSGLARFEPLRVYLDALCQITLQCSA
                     AEGEAVFAFCAMDYYHRGAGHVRGGIGALAELLLTACTRNGVDVRLATRVKGIEPVPP
                     EAGRFKVQTRRGDVYADHVIANVLPSQLASWLGPSSASPTSLQQLSKRVEAGYGAAML
                     YGLLGGEPSNAATPFHTSSPPVRHRQLVLDVERPFVEGNHVFVSLGELQPGLAESDDA
                     RRSLTMSTHLPLPTLQARGDAAPSYVAEVQARMRATFERLCPDWALRLQHSFTASPRT
                     FARFVGRSRGAVGGIPRRAGLDNYRQLGPYRVRPGLWLVGDSVFPGQSALAAAVGGIR
                     TAAAVARCAGIPRSADVLGGQVDSTASLPPA"
BASE COUNT         2957 a         4693 c         4585 g         2728 t
ORIGIN      
        1 gacggtgcac tacgaccgcg cgattctgga ccagggagac tcgtgtccgt cgcccacgaa
       61 tgcagggtgc gccggctgtg gagagtgcgg cgcgggcctt acgtgcatgg ccgaggagcg
      121 cgatgcgggc acgtgcgcga gttgtgcaac ggactcggat tgctgtagcc ctttggtgtg
      181 cgataacggc cgctgccgag cgttactcac cgagtagtcg tcatcttttt gcgccccggg
      241 tatcgcgaaa ctcggaaccg atgctatcct ccggagtcgt tgcttcgtaa gcgagtatcc
      301 gagctcgctt cgacgttgcg actcgcctag ccgattggtt tcgaacccgt gcggctcgat
      361 gagtagccca gcgttagaca gcgcagcgct ggagaaacag cgcagcgctc gagaaacagc
      421 gcagcgctcg agaagctcga gaaacagcgc agcgctcgat aggccaacgt ggccttacgc
      481 ctgatggcag cagcaccgaa tagccctgaa cggcctccga gcgccgactg gctatcaccc
      541 gcacaactaa accggcctgc gctaacccgg tctatacagt tcaagtggtg tgcggcggcc
      601 cgatcgggca cccttctgtg acgactcgtg cgattctatg cagactcgtt ccttcgagat
      661 gtcgtggccc agacgctaga cagcctcggc aaaacaatct aaccgaatcg aacgacgagc
      721 tctcacttcg aaactccccc gtgattcctg ctcagttagc tagtaaacgc gggtaagctc
      781 aatcaggcac tcaactccaa caattggcgc tcccactcct gccaagatgc agccggccac
      841 cgaagctcag ctccaaaaag acgtagcgtt gatgagacgc gccgctctag gtgatcctgc
      901 tgcccgacgg gaagcggtgg tccgcgtttt gccgcgcgct cggcgcctgt gccagtcact
      961 gctcggaagc gcgagtgact ccgaggacgc gagccagatg ggtatcgtcg aggtgctgag
     1021 atcagccgct cactttcgcg gggagtgccc cctggagcac tgggccagcc gcatcgttgc
     1081 ccggacggcg ctacgcatgg ccaaagatga gcgccggaag gcccgcatgt cggctgcatt
     1141 cttggccgag ggggaagagg acgccgtcga agcagagtac ggcccgtcgc gcattctcgc
     1201 gaccgagtgc ctgaagaagc tgccagaggt acagcgaact gcgctcctgc ttcgctgtgg
     1261 cttcgaatac tcgatcgagg agatcgcgga actgactcag gtctcgcgca ataccgtcaa
     1321 agaccgattg ttgcgagcga gggagagcct gcgcgatctt ttgcgccaga acgcagaatc
     1381 ggatcaagat ccgaccatcc ccggctcggc tcggctcatt cggtgagcga cgctcgttcg
     1441 gtgagcgacg ctcatacctc gatgctcagc gtcgagccgt ggtgatgagc gtcggtccta
     1501 gccggcgagc tccggaacgc gaatgttggc ttgctggaaa agcagctgta atccagcctt
     1561 atcgttgctc ttcgacaagg cttcctcggg tgccacgacg ccgtccttga cgaaccggat
     1621 gaggctgtcg ttcatcagac acatcccgac tttgcgtgcg gtctgcatca cggtcggaat
     1681 ctgaaagatc ttggactcgc ggataaggtt ggaaatggcg gggatcccga acatgacctc
     1741 gagcgccgcg acacgccctc ccccctgctt cttgcacagc atctgacaaa tcaccccctt
     1801 caggctattc gcgagcatca cgcggatttg gttctgctgc tcggcggggt attgatcgat
     1861 gatgcggtcg accgtggccg gcgcggagga ggtatgcaaa gtaccgaaga ccaaatggcc
     1921 cgtttcggcc gtctcgagcg cgatcgcgat ggtctcgaga tcacgcatct cgcccagcaa
     1981 cacgatatct ggatcctcgc gcagagctgc acgcaaagcg cgcttgaaag aatccgtatg
     2041 ctcgcccacc tgcctttgat tgatcaaaca tttcttgttc gggtgaacga attccacggg
     2101 gtcttcgatg gtgatgatgt gttgggagcg gttgctgttg atataatcta tgagcgcggc
     2161 gagcgtggtc gactttcccg agccggtggg gcccgtgacc aatacgagcc ccttgggcag
     2221 gtgacacagc gacagcaact ccttcggcaa gttcaaatcc tgcgcactca ggatcttact
     2281 gggaatgacc cgaagcaccg cgcccatgcc gcgtagatcg acgaagatgt tggcccgaaa
     2341 acgcgcgcgt cccggcagct cgtaagcaaa gtcggagtcg tggctgcggg tgaattcctc
     2401 gcgattgcgc ggaggcacga ttggcaacaa catacgttgt atggcctgag gagccagggg
     2461 cccatagccg cgtacaggca ccatttctcc gtccacgcgc aacatgggcg gcgcgccgct
     2521 cgagacatgc aaatcggaag ccttgagctc cagcatcttg ccgagcagct cgtcgatctc
     2581 ggcagtgctc ccgccactca ttccgtcgtc gtagagctga tccacgctgg gcagcggagg
     2641 ctgggtcgcg agcggacgcg tgggctccaa gcgaaccgtc tcgtcaggtg gccttcgagc
     2701 cggcacagaa acggcaaatt cggggctcgt gaccacggcc cgcgcgactg gtacctcgcg
     2761 tggcacgggc cgaggcccca aatcctcccg tggcgcgtcc tcgcgtccgc gagagatggc
     2821 gcgagcgtcc tcggaccgtc cctcgtcgca gcgatcgaga aatagggaca cgtggggcaa
     2881 gcccatcttc atctcgacgc aggctcgcac cggccctcta ttttcgagcc gaaattccca
     2941 ctctgcccaa cccgaagcca acccgcgacg ggcttccgcg gtcatgacgg gagaaagcaa
     3001 atccaggata cgctccggcg tctgcgtgtc ccccttcact tcgctttcgc cctgagcgag
     3061 caccacgatc gtccgccgac cggggacgag cttcacctcc agcgccccaa ccatcagggc
     3121 gcgtttcaac agatccgtga gcgggctatt catgcacagt cctcgaagct cgaatgatcc
     3181 acgagcggcc taccgagggc aaccccccgg gccgtcctca cgagagcgcg gcgcacgata
     3241 cagacacggc ctgataagcg ccctcgacct acccggagcc ggtctctgga tcagcttgga
     3301 gccaaacggc ttcgaagtgt tctcgatcgc tgttccatgc tctaaaatcc cgagagccac
     3361 gcacagcttc gagtaagttg tgcagagcta tcctgacgtc gggcagaccc cacacgtctg
     3421 ttgcttcatc tggatgattg aggcgcaccc gctcgccgac ccgcgtgatg accactcccg
     3481 tcatgggcgg cacgctacag ccaagtgcgc acagcacgcc ctggagacga cacagtacgc
     3541 tgaaagcgcc gacctgatgc gcggagacca acacgaccac gggcttgccc aaccacaccg
     3601 aagtgccctc cgtctcggtc atgcgctcga gaaacgcttg gagcacgctc gaccaactgg
     3661 accagtacgt cccggtcacg aagaccagcg cacgcgccgc ggcgatctgt gcggggccga
     3721 tcgaggcgac gttcgccacc gcgcacgcca ccccctttcc cctcagctct tgctgcacga
     3781 actcgcacaa aacctgcgaa ttgccacgtt cctgggccaa accagcgttg aggatcagca
     3841 cctcgagagg ttccagctcg cgcgtcattt cgtctccttc tattctcttg gggctctctt
     3901 cgatcggcgc attttgcgtt tagcgcaggt gcgccttccc tagcacgggt gctgcttcag
     3961 cgcggatccc cgagagcttc agttgctgac ccagcgccga gcggcatcca ccgagatagg
     4021 ctgccagctc tccgtgaagg ggtcccattt cgaaaactcg ccgctcgagg agtctcggtg
     4081 cagcaccacc gtctctgccg aaagcgggcg actcggccgt acattgcgcg aatccgcgtt
     4141 cgtcaacacg acgaccaaga ctttgtcact atctttggcc gcgacgcgcg cctcacgtag
     4201 cgcctcggaa tcgtgcacca cgcgaggccc cccctctgga tctggcgtgt ccggaacgga
     4261 tctcacctcc accaccgccg agttcgtgac agcgtcgacc tgcacccgtc caccgccgcg
     4321 actaggcaat gaaacatcct ccccgcccgt tcctgcgatt ccgagtgcat ccaacacgga
     4381 ggactcttcg tggccgccgc gatcggcatt ttcccgcaga cgctgatccg cacgcaccca
     4441 gtccgtagcc gacaggggag tgcgacccgc gttttgcgct ctgcaaacat aacgctcata
     4501 gcgggcttga ttggcgaccg tctcgctttc agcgtcatcg agccgttcgc caaagtgctc
     4561 ccgctgctgt gacgtgagac cgtctggtcc tacgctcaac ccgcgctcac atttcggggc
     4621 ttgcggctct cgagaatcgt tcgccaggtg gcgactggtt ggcggcccgt cgggatcgcc
     4681 aatgggagtt ggcgaatagg tcgccactaa ctcgcgcaag agctgacccg aatttggcag
     4741 cccgcggccc tcgatttgcg ccgcgttcac ccgcaagtct gatcgcaggc tctgtccata
     4801 tcgtgtccag ccggccatcc cgaaaagggc aacggcaccc aagagcacga gatgttcgag
     4861 cagggatgct ccgcggcgcg cgtgggctga gttgatgcgt cgcaggtgcg ctcgggagcg
     4921 ggacggcgac gatagctgaa ggacgctgtg tctagcggag caaggccggt ttgtcatgtg
     4981 attgagacat ttggtaggcg ctggctccgc cgtcggcagt tcctcgctgg acagcagcac
     5041 cagcccagag cgcgccaacc ccttacgagc gacgtgcccc cgaacacacc tgccttgcaa
     5101 aactggtgca aagcacactg gccgctcgac acgactgtcg aaggtctagt gcgcataaag
     5161 acttggactg cgtacaaacc tggactccga acaatgaaag actccaaaga catccggcga
     5221 aaactcacgg ccagcattga agtggcaatt gcccgcggag cgaacagccg gacgtcaatt
     5281 tcaagggcga cgactttgtc gcgtcgccag ttgacgtgga acttgaactc gaattgatgt
     5341 ttcgggtcgg cacactatcc cccagggctc gcctgcgccg ctgcttggag acacatcctc
     5401 gcgggtgcag actgtgcgac acagtatcca gtttggtgct tgttcacgaa ggccaacctg
     5461 aggcaccatt ccggcaaaga ggcttctgca gatgcgtctg gttccaggat tgcgtgcgcc
     5521 ttcgttcgtc atcaaggcgt tgactgggca gacgtacacg ctcgagcagt tgcgcggcga
     5581 gctcgtttgg ctcgcattct tccgctttgc gacctgcccg ctatgcaacc tgcgcgtgca
     5641 tcagctggtg gacgagtggg aacgcacgtt tgcgggcaag atccacctgt tcgcaatctt
     5701 ccaatctccc aaagaacgct tcacgaacta cgtttccaag tatgaaccgc ccttcgagct
     5761 cgtgtccgac gtcgagctgg ccctctatca gcagtttggt gtggagacgc gcataagcgc
     5821 cacgttgcac ccaaaagtcg cgaccaccct gctggaagcc cgtcgcgcgg acattcctct
     5881 attcgatggc cccaaggatg gaggctcctt tcgagtgcca gccgacttcc tcatcgatcg
     5941 gaacggggta ctgcgtgttt gccgatacgg cgagcatctc gcggatagta ttgcaagccc
     6001 cgatgttctc acgtttattg gagagcacgc ccagacgtcg gtcaacccgg gctcgctccc
     6061 gcctcccgca cccacgacta ccccgcctca gtcgctgaaa acctgattca ttgcagagca
     6121 atcagcgcgg cccagtccag cacggattgt ctttcaaggg gcgctggcgg tccatgcacc
     6181 ggacggttcg aaccaagcag caaaagcgca aggaccgaag cgacgccttc gtccgatggg
     6241 tccgcggggc gagcacagcg gaccagcaca gcgaaccagc gccgcgtcat ctagacgtgc
     6301 agcgacagcc gagagtcgca actagaggac cgcgctgtta cacggcttct tgaccgtttc
     6361 gactctaccg accaaatgcc gatgggactg cttctggaga acagcgcagt tggctaccag
     6421 catgtcgccc ggcctggaat accgcgactc aattcgtaac aaagaaagtc gtcacttcga
     6481 aggatttcgc gtctaagaga gtctaagagc aagtcccaag aagaagagag taagagcgcc
     6541 agagcgccag agcgcgagag acggaaacag ggactgatga gcactgcggt agtaagcacc
     6601 gcggtagtag gcactgtaag cactgcgcca gtaagccccg taagcactgc ggcggtgcac
     6661 acggtcgtga gtgtcgctga acgcggtggc caagtcatgt gaacccaacg aacaccgtgg
     6721 cgcccgagtc acctatcgag tgccactgaa agaccaggtg aatgtgcctt ccggaaactc
     6781 gtgtgaagag accgagagaa ggacctcgag agatgcgctc caccccggta gctctcgaga
     6841 aagcgagcgc ccccacccca ggaagcaggg ggacagtcag cgctgagtac gcacgagtag
     6901 gctcccgtga tcgctcgttt ggtaggctcg cgattccgac gcaagcgctc ctcgcgcaag
     6961 tagggatgga gttgacgaag ccctcgcaaa gtgtgatgag gctggtgaag agggtgagga
     7021 ggttcgtccg ctggctcagc gggcggccgc tgcacacccc agaggtgaag ctatgagtac
     7081 tcgactacgt attggtatcg ttgggctggc tatcggtgtg tccgcattgc tgagcatggc
     7141 gccgtctgcg gccgccgcgg attcgaatcc accgctcgac ttcgatcacg gcaacgctgc
     7201 catcgaaaag gtgatcccga gcgtggtccc ggtaatctat gaaatcgtcg ccccaagtga
     7261 tgcctcgatt gtgcttcgga tcactaccat tgcatcgaat gcttggttcg atgcaatcgc
     7321 cccgtatcat ccgacggcgg ttggcgtctg ttccaagctc ggccgtcgac cggcggcgga
     7381 gcgcacacag cgcaacaaga acgtcgctat cttgtacgcc tcgtaccagg ttctgaatag
     7441 cctgctccca aactacaagg cccggtggca agctctgctt agaggtgaag gattggatcc
     7501 aaacgatacc cgtgaggacg attcggcagt gggcatcggc aatcgggctg gcaaggccgt
     7561 tgcgaagttt cgcgagcacg acggcatgaa tcagctcggg gacgaaggcg gccgcaagta
     7621 caaccgcatg ccctatgccg actacacggg gtacgagccc gtaaatactg cattcgattt
     7681 gcgtgatccc tcacgctggc agcccaacct ggtcacccgg gggacgggcc tctttcaagt
     7741 gcagcacttc gtgactccgc aatgggcatt cacgcggccg tacagctaca gcaatcccaa
     7801 ccggtatcgc tcccctgccc ccaccgcgag ctgggttcgc aactacgcaa gctatcgccg
     7861 gcaagccgag gaggttttgg cggcctccgc ccatctcacc gacgaacaga agatgacggc
     7921 cgagctgttc aacaacaaga tcgagagtct cggctactct gctttgtacg ccagcgaggc
     7981 gttcaatttc gatctcgacc gcttcgtaca gtacgacttc ttgaccaatg cggcggcctt
     8041 cgacgggggc atcgccacct ggaacgagaa gtatcggtgg gacaccgtgc gtcccttcag
     8101 cgcaattcga tatctgtatc gcaagcagga agtcaccgcc tggggcggcc ccggccgggg
     8161 cacggtcacc gacatgcctg gtgtggagtg gcaatcctat ttgcctaccg cagatcatcc
     8221 cgagtatccg tccggctcgg cctgcttctg cgccgcccat gcgcaagcta gccggcgttt
     8281 cctgggtgcg gataatttcg ggtggagcgt gccgtttctc aaaggttcct cgaagattga
     8341 acccggcttc accccggccc gggatgtcgt gatctctttc gaaacgtact ccgacttcga
     8401 gaaacaatgc gggcagagtc gcctctgggg gggagtgcac ttccaatccg cgatcgatgc
     8461 ggggcgcgcg ctctgccgtc aattcggcga tctcacctac gagttcgtcg ccgcgcatat
     8521 tgcagggaaa acacgctaac tccgtggcaa ccgagtgcgg ggtggggcgc cgcccgcggg
     8581 cgctccacca gcgctcgcgt gctcaggcga tcgttttttg ccgttgcgca ggctcgtttt
     8641 cgctgcagga aacctcttgc gcgaagcgcg cagtaacgaa acagtgcaat gcattaggca
     8701 acggagagcg taacggattg gcagacccca cgcggcacac cggagaggct gggggcccct
     8761 ccgcggtcgg gcacggcagc gtgcgaccgt actcgtacgc ctggcgtgcg agcctcgccg
     8821 cggctctgtt tttcggcttc tatgctctgg cgttgagctt ggccgggggc gcgctctatt
     8881 tggcgtacgc cagcatctcc tcggctcatc ccgtgttcgt ccagtccgcc ttgggttgcg
     8941 cgctgtttgc gttcgccgtg ttgaaggcca ccttcttcgt caagcgcccg agcttcgaag
     9001 caccgggtcc cgagattacg gcgagcgagg agccgaaact attcgagctc attcagagcg
     9061 tgtgcgcgca gatgggcacg cggatgccga cgcgcattta cttgatcccc gatgtgaacg
     9121 cattcgtggc cgaggtgggg ggcttcatgg gcctggggtc gcaacgcgtg atggcgatcg
     9181 gagtgggcct gctagacgta gatgatctct cggagctgcg ggccacgatc gcgcatgaat
     9241 ttggccacta tgtcgcgggc gacacgcggc ttggcagctt catttaccgg acgcgagccg
     9301 ccatcgccca ggtgctgacc tctctgggcg gcagctggtt ctccaaaccc ttcgagtggt
     9361 acggcgctct cttcatgcga gtgacgcaag cggtaagtcg agcccaagag ctcgcagcgg
     9421 atcgcagcag cgtgacgctg gcgggacgcg atgcgctggt cagttcactc cgacacgagt
     9481 cgcgcgcggc cgtgctgttt caacacttcc tgggcagcga actgacgcca ctgctcgaag
     9541 cgctgcaccg cccggacaat ctgtacgagg gcttccgggt cttctggcat gctcgcgtcg
     9601 agcagggcag cgtggccgag ctagatgtgg ccctcgagaa cgcggggacg acggtttacg
     9661 atacgcatcc cgcgctcgga gaacgagttc gttttgcgga gagcctgagc gatccagggc
     9721 tcgcccgaga ccgcacgccg gcccgaagct tgctgcggga tccggaggcg accgagcggc
     9781 gcatgaccga tcacatcttg gcctcgacgg ggaatggtcc cctcacccgc gtctcgtggc
     9841 acgaggtgac ggccaaggtc tatgccccga agctcagcgg ggacgcccac ctgaccgcag
     9901 gagaggtcgc ccacgcgctc ggcggagccc ccgacgtctc gggtgcattg atgacgctgc
     9961 tcctgaatct gcgcgggatt ggggtgaaaa cggtcggaga acgcctggaa ccgcgcttga
    10021 gcgaggtgcc cgcgcagacc cgagacgacg tttcgatgcg tctcctcacc ttcaagctag
    10081 gcgtcctttt tggccaagcc ctggtcgagg cggggggaga gtggcgctcg gacgtcgggc
    10141 accctcatcg agtcctgttt cggagtgagc cgctgaagcc tttcgagcta gcgcgggccg
    10201 cgctgctcag tcgagacggg ctcgtggagc tcgagcgcag gtactgggac gcccgacgtc
    10261 gggacccgat cgcctgaagc ctgaaaccgc ttccgtcatg ccccggaacc tgccggaaac
    10321 gttacgcagg tccgaaccgc ccgcctgccc cctcggtcga gcaccccctc cgttcgaaaa
    10381 atgctcgcga accgcgccgc ttccgggcta ggaaaccgca tggccaaaga gacaatcgta
    10441 tccgacttca agcttaaagg tcgtgagatc ctgtcgtcgt acgcgaactt caccatcgaa
    10501 ggtgaaatga gcgcgaaggt gcgacgtgga gaggagacct tcacgattca ggagcgcaac
    10561 ggcattccga agggcacctc ggacggagag aacgaagccg tctatattcc gaacgacaaa
    10621 gccatcgagt caatcagcaa gaacattgct ccgttgctcc cggctcaggt cgtgatccgc
    10681 agtcccaagg acatcctgga tgccgcggcc gccttcgatc tggagttggt caaacgcgcg
    10741 gggcacaaca agcgtgactg gggtggtaat gcctgcctgg ctgcgtcgac cgcgttcctg
    10801 caaactctgc tgaaagcgag cggatacgaa gcttacgaag tgttcctcac ggaacagagc
    10861 aagaccttct acttccccaa catcgccttc aacatggtgt gtggcggcga gcacgtgccc
    10921 ggcaccaagc aggacatcca agagatgttc ttcttcgcga tgaaggccaa gtcggtcaaa
    10981 gaggtgttcg agatcggcac caagttcttc cgtcagttcg agaagaatct cgtgcgcgac
    11041 ggcttgccca ccggcaccgc caaggagcgc ggcttcgtgt tcccggtgaa ggacaacttc
    11101 gaaggcctga cccgcatcaa agagtgtgca ggccagctcg ggctgaagcc ctccgactac
    11161 gcgatcggca ccgacaacgc cttcagcgag gtgcagaaag gcgaggagct ggtgaagaag
    11221 ggcatgtacg acatgcgctt ctcgggcacc ggtctcaaga cgcgtgagca gctcgtggac
    11281 ttcgacggaa ccgtggtcaa cagcgccgag aacttcgcca ccatggaaga tccagggtcg
    11341 gagcacgacg tcgaggcgca caagctgatg aacgcgcgct tcggggatcg cgtgcagatc
    11401 gtattggacg atctggtcgt gacccagctg cgcttcatca ttccgtacct gtgcggaaag
    11461 aagcgcgagg accgcgccgg taacagcgtg ttgatcaagc tgaaccagtc gggaacgttc
    11521 accgagacgc gccttgccgt cgaggtggtg ctcgggctcg ccgacgccaa gcaggtggag
    11581 cagttcctga gcgctcgcgg tgatttggat ttcattctga gcgagtgccg caaggtcggc
    11641 gttcagagcc tggcggaggc cctggagaac atcaagagta acgggttcac ggccttcatc
    11701 tcgcaccgct ccacggaagg ttcgagcgag ttcctgccct atctgccctt gatgtacggc
    11761 gcggtcaccc gccgattgtg gttcaaggcg ggcgctccga acggcgagcg caatctgcag
    11821 aactacaacc cgctcatccg agctgaagag cagctgcgcg cccgcggaat tacgaccgaa
    11881 gtggcgggct tcgccggtct gccggctggg gtgaaggtcc cggccgcgca gtgagttagc
    11941 cggctgggtc gacagggtat cggtcgacag cgtatcggtc gacccagcgt cggttaatcc
    12001 ggagtcgggt cttcatcctt cggatcgctc actcggtcgt gcgtcacgac ggagtgagcg
    12061 gtcgaggcga atccagtggg cggcgcctcg gatggctcct cattgtcacg gacttccggc
    12121 gaggtcgcgt ccgccattgc ggcctcgcca atggccgtta gctcccgcgc ggccagatca
    12181 gccggggttt cgaggctcac tcgtccgaac accccggaac gaaactcgtg aacgagaatc
    12241 tcggaagcgc gacgccagtc gaactggccg cctttgcgta ggcaaccgta cttgcgagcg
    12301 attgcctcga gcacgagctc ggaagagctc ggcagctcgg ggatcgcgag gcgcgccgcg
    12361 aggcgcttcg gataatgctt gctcaggaac tgggccgcga acgccgccac cgtctcgtag
    12421 tccatctctg cttccggcag cgcgccaccc aagctgaggc gtagtacgga ctcggtatcg
    12481 atgcgcggcc acagaatccc tgcgttatcg agcagcgtga ggccggtttt gagagtcact
    12541 agttgaggcg cgcgcgtaac gccgggctga tcgctcactt tggcgacctt gcgctccatg
    12601 agcaggttga tgagcgtcga cttgccgaca ttcggaatgc cgacgaccat gacccgcacc
    12661 gggcgcgctg actgcacgcg cttgggcgcg agcttccgac acagctcggt gattcgagcc
    12721 cgcgcctcgc ctgcacggtc tttgctgaac agcagagcga cgacctgcga ctccgcttgg
    12781 ctggcgaaat ggtcgaccca ggcccgcgtg atctcgggat ccgccaggtc cgtcttgcca
    12841 agcagcttga tgcagggctt ctgcgcgcgc agcttggaga gtacgggatt cgagctgctg
    12901 atgggcatac gcgcgtccag cacctcaatc actacgtcat tcgagggcaa agcttcggcc
    12961 atcgcgcgac gggcttgctt catgtgccct ggaaaccact gcatcgacat cgtcagctgc
    13021 tgttaccacg agatcgcgcg cgaaccccgc ctgtactcca tcctcggtgg tcgcctgcgg
    13081 gagaagccgc aagaaacgac cggctttttg acccgaaagc gtgaacgaac ctgagcgcgc
    13141 gaaatcaacc cactgcccac ccgcacgccc aaaagcggat tcagggttgt gaatcgccga
    13201 gaagggctct aacaatcgaa ggcgtttgcc aacgaggagg ctttcccgat gcgctcaagt
    13261 attttcgtgc tgtcactgct ggctctttcc gcatgcaaca atgaccccac caaaggcaaa
    13321 ccgcaggcca cggtgagctc gccgctcgtg acgtccaccg ccgcccctgc gggaggcacg
    13381 cctgcgccgg ctggtgtgac tacccagtac gcattcagca acgagggctc gcagcttgcc
    13441 ttcgtgggcg cgaaagtcac gggcaagcac gacggttcct tcaaagcatt ccggggaacc
    13501 atcagcgtgg tggacaacga tcccgccaag agcaccgtga cggtggaagc ggactccgcg
    13561 tcgctcgata cagataccgc caagctgaca ggtcacctca agtctccgga cttcttcgat
    13621 gtggagaagt atccgaaggt gaaattcaac tcgacatcga tcgtaaaagc gggatccggg
    13681 tacaccgtca cgggcaactt ggatttgcac ggcgtcacca agtcgatcag cttccccgcg
    13741 agcctgcagg tggcaggcga tacggcgacg gctgatgccg agttcgggat caaccgcaaa
    13801 gacttcaata tcgtatacgc gggcaagcct gatgatttga tcaaagacga ggtgctgatc
    13861 aaactcaagc tggtggcaaa gaagcagtag agtcaacttg gccgccgagc acgtctgcac
    13921 tgcgagggat gcctgcgcag cgtgcaaccg cggcagccgt acggatgccg cctaccgcgg
    13981 cggccaaggc gctctggcct ggaaacaccg agtctcctac gagccagagc cccggccgca
    14041 cgcgataggg gccgagctgt cggtagttgt ccagacccgc gcggcgcggg attcctccca
    14101 ccgcgcctcg ggagcgcccc acgaagcgag cgaaggtgcg cggagaggcc gtgaacgagt
    14161 gctggagacg taaggcccag tctggacaga gtcgttcgaa ggtcgcgcgc attcgcgctt
    14221 gaacctccgc aacgtagctc ggggcggcgt caccgcgcgc ctgcaaggtg ggcagcggaa
    14281 ggtgagtcga catcgtgagg ctgcggcgag cgtcgtcgct ctccgcgaga ccgggctgaa
    14341 gttcacccaa ggacacgaat acgtgattgc cttcgacgaa tggccgctcg acatcgagga
    14401 ccaattgacg gtggcgcacc ggcggtgagc tagtgtggaa cggagtggcc gcattgcttg
    14461 gctccccacc caacaagcca tacaacatcg ccgcgccgta gcccgcctcc acacgcttgc
    14521 tcaactgctg gagtgacgtc ggcgaggcgc tgcttggccc caaccacgaa gcgagttggc
    14581 ttggaagtac attggcaatc acgtggtctg cgtatacgtc gccacgtcgg gtctggacct
    14641 tgaacctccc tgcttctggc gggaccggtt cgataccttt gactcgcgta gccagccgaa
    14701 cgtcgacccc attgcgcgta cacgcggtca ggagcaactc cgcgagagcc ccaatccctc
    14761 cgcggacgtg acctgccccg cggtgatagt agtccatcgc gcaaaaagcg aacacggcct
    14821 cgccctctgc ggccgaacat tggagggtga tctgacatag cgcgtctagg tagacgcgca
    14881 gcggctcgaa tcgagcaagc ccactccgct ccaagatagc gcccaagggg ctgcccaagc
    14941 ggggcaggag cggcagatac tcc
//
LOCUS       DASTKU010000947        20771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1973765, whole genome shotgun sequence.
ACCESSION   DASTKU010000947 DASTKU010000000
VERSION     DASTKU010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 20771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20771
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1973765"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VFQ61_33515"
     CDS             complement(<1..932)
                     /locus_tag="VFQ61_33515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959474.1"
                     /translation="MATLLSRLDRGQDLSWLAERPNIQGLLADALRVPVDDLRRAVGE
                     PAPAQDEVRIRRLRDLPTARALDLSSEALPPGIPSEVLGGSSRPVWWVAPSGSGRSLV
                     GQWLQARGRAKFLELGEPGTELPTELLGKLAMSDALFIELTGHSQGLEAQRVIAPRPG
                     LCVAAPFEPPASSEFQVVESPALAGILPELLEWVADRLPLQSQFDAERLLPSLKELVE
                     SRQLPSLGAVLGYVGLVDEFGIKELNHRSLSRLAKRFVEHRVSRRVDPALPYASWVRR
                     SIHAALLGIAERVLSESDVPLETPRSLDDWLALVP"
     gene            1377..2075
                     /locus_tag="VFQ61_33520"
     CDS             1377..2075
                     /locus_tag="VFQ61_33520"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET9959475.1"
                     /translation="MSGIAEFLVWYLVFVFSTTCHEAAHAWVARRGGDSTAYALGHVT
                     LDPLPHIRREPFGMVIVPILSFFQLGWIMGWASVPFDPLWGARHPKRWALMSLAGPAT
                     NFLLAAMAFAALRGLSSMNVLSLTDAGGVTVGLVQLPPGYALNSPLGALGLGLTALLK
                     MNVILGLYNLLPVPPLDGAAVVEGFAPRGLRSLYDSIRRNPMLQLLMLLGAWYVFPTL
                     LNPVFRVVLRSLYS"
     gene            complement(2133..2597)
                     /locus_tag="VFQ61_33525"
     CDS             complement(2133..2597)
                     /locus_tag="VFQ61_33525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959476.1"
                     /translation="MVGSRDGSRTEPAQVVGTTRRKNLRQKSLRFASGAEERVAAAER
                     AQELELQLERPKVRADCSDGLRPCPYVGCRYHLYLDVTPGTGTIRLSFPELEVWEMQH
                     SCALDVADQSGISAEHVSVLMNISRERVRQIEAAALSKLERRREVRVLADPA"
     gene            complement(2698..4653)
                     /locus_tag="VFQ61_33530"
     CDS             complement(2698..4653)
                     /locus_tag="VFQ61_33530"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9959477.1"
                     /translation="MLTNPATDVFIGAAIESETESGVRFVLERRLGLGGTAAAYLAWR
                     VSPHGESPAVLKIILPEIVQGHGPKAVTVVRKESVALGRLNEQVPPTPFVVRLMDGGT
                     VIHQHRGAALELPWLAIEYVDGGIEGTTLEDRVRHSIKLTGRAFERERAARVVGHVAS
                     GLTDVHAVGVIHRDLTPNNVLCCGFGDSELFKISDFGVAKPAGMADTFGPAVMGTPGY
                     ICPEQIQSNTNGPAGDIFSLGCLVFYVLTGEHLFEASNAIEMVQRTSEPRRRSIAEAA
                     ALVDELRLDPDACAAIDAVIARATSVDPAHRPQTPRELAAGLVPVLLERATRAPRKER
                     ATLMSVGQDSVLPQLSWIVRHPPGDGRIVRSVGWDGDGHCLAATTNGLEYWNGSQWQP
                     APTRDLPPGLGLRFVRRVAPGRWLVGGDEALVAEYSHAGVSRVIRGKDPAVSFVDASG
                     SAGEVVVMVGLRDESTPELWAAVSGRWLKPLPLNEVAYVAGLSQIDEEHWLVVGRSRH
                     GTGFAALYSPLSWELNKLNAPATRAWVACTSRSEREAALAVGAEGAVLRLERGSVSGD
                     VIPERPNLASVSIDVLAREWAGASGELWCATGGVWQRVFAEEQWGRPFISVFADVASV
                     VAMSVDGGVLECRTVMPSRSSLRPPPR"
     gene            4893..6209
                     /locus_tag="VFQ61_33535"
     CDS             4893..6209
                     /locus_tag="VFQ61_33535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HET9959478.1"
                     /translation="MVRRQNRLSAPFTNLLQALQALPLIPTRAGEGLGLMVGLLALAC
                     SSEAEPAVEQVVDRPIVETVDTPLPGIGREFTRDFNDGDLAFSTPLHAADGLGPLYTR
                     ASCAACHDGGLRGPGLVQKMSVVEADGVTPAADQGKLAYGHTVHPLTSPAGELGITPP
                     VDDSSVKISERLGPAVIGRGYMEAVLDSEIERVAEEQAARTDEIHGRVNRLPYASEPN
                     VDKRFHDYKKGDLVIGRFGLKARIATLDDFTADAFQGDMGITSPLRPDEFKNPNALED
                     RKPGVDVSYASVNMRANYVRVLEIPTRRGPQDAARLFQDVQCAACHVPSLRTRADYPI
                     DALADIDAPIYTDLLIHNMGEDLADGLPAADDVDGQAGSFDWRTAPLIGLRFNRTFLH
                     DGRAKSVEEAILMHRGVGSEANDSVARFEALSERERALLVSFVESL"
     gene            6221..7264
                     /locus_tag="VFQ61_33540"
     CDS             6221..7264
                     /locus_tag="VFQ61_33540"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HET9959479.1"
                     /translation="MRHWSIIGVLGVLLVCGCSDDSDSPAPSSESRVTSEVHSLLLTN
                     ISALHESASQLQAAAPAHAWDAERDAEAISQMTEAWLKARSEYEHIEGAVAPLFAEID
                     VAIDARYEDFLETLGKEGDGNLFDDEGVTGMHAVERILFAKEMPDSVVQLESSLPGYT
                     PAAWPSTDEEAVAFKKQLCARLVADTQELLTQWKPSRIAVDGAFRGLIGLMNEQREKV
                     NKASTQEEESRYAQRTMADLRDNLAGTEKVYGVFQSWILSKSGGAEVNEKIERRFGEL
                     KTTYASVKGNAFPAAPATWSAEDPSPDDLATPFGKLYSAVHEAVDPNREDSLVSAMNE
                     ALVLLGYEEFVEE"
     gene            7285..7818
                     /locus_tag="VFQ61_33545"
     CDS             7285..7818
                     /locus_tag="VFQ61_33545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959480.1"
                     /translation="MTVLRSIGCLLATLVAVGCGSGGSGADSTEEAGSFLAFAGDFQG
                     FRDWSSFDVTGESALTEVHQGQVIIYVSDLPPPGLTQFPTGTRIVKEAILEGQPTQIF
                     AMAKRGGNFNSGAPGWEWFELEDVAEKNGVKINWRGFGPPAGEKYAGDLESECNTCHR
                     ACGNDSVCTTHLDLSSF"
     gene            7909..8724
                     /locus_tag="VFQ61_33550"
     CDS             7909..8724
                     /locus_tag="VFQ61_33550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959481.1"
                     /translation="MRLRLSLLAAGLGVCTWSASAIANPRPLPFTYQHEQLAQGDSEI
                     EQYADYTVARARDATTGDPVYYGLTAFQTEFEYGILPNLELGLYFTFVPTTNVGFVDT
                     PRPPQGNGLKQRLRYQFAPTGVWPVDLSAYLEVVETEREIELEARLIVQRRFGRLRAI
                     VNLSGEREFYLNGERDLVAAPSAGLTYELTPAIQPGIEWWMRAEYPEQNPPSPRPFEL
                     GPHQYVGPVLLLSFGRLWWTTGAYARVSDLDHGIRAGESYGKFWARTIVGFGL"
     gene            complement(8767..9909)
                     /locus_tag="VFQ61_33555"
     CDS             complement(8767..9909)
                     /locus_tag="VFQ61_33555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9959482.1"
                     /translation="MSASALAFDASMNWRPELLLVDDEALNLSALAACLEPLEASIST
                     AEDGHTAVRRFEESEPDLVILDLAMPGLDGIEVLRRIRSHATRYDTPVILLTGHAERE
                     HRLRGLEAGADDYLEKPLDARVLLARANTLLRLKQSRDLLLIRNAELERAQREQRELT
                     EFIVHDLKGPLTGIVANTEWVYEQLQRKELALLRALEDVLGSAGRLRSMINDLIAVSQ
                     LERGTFPVKPRKVQVGPILRSVTNGFARAAENRKIALVESPENAAFVQVDPSLIQRVL
                     ENILDNSLRYTPESGRVFIGAEVQNEIQIVVANSGPAIPEAERKLIFEKFTRGSNAGV
                     RTTGNAGLGLYFCKRAIEAHGGDIEVIQTDEYPTCFVIRLPQQQAS"
     gene            complement(10259..11821)
                     /gene="proS"
                     /locus_tag="VFQ61_33560"
     CDS             complement(10259..11821)
                     /gene="proS"
                     /locus_tag="VFQ61_33560"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET9959483.1"
                     /translation="MAKNPSAKTAISPTRTEDYAQWYQEVVRAADLAETSPVRGCMVI
                     KPWGYAIWENIQRVLDGMFKATGHKNAYFPLFIPKSFLEKEAEHVEGFAKECAVVTHH
                     RLVAGKDGGLIPDPEAELDEALVVRPTSETIIGAAFAQWVKSYRDLPLLINQWANVVR
                     WELRTRLFLRTAEFLWQEGHTAHETEEDARRETRQMLDVYATFAENYLAMPVIRGPKS
                     DSERFPGAVDTFAIEAMMQDRKALQAGTSHFLGQRFAEASGIRFLDKSGQQRLAWTTS
                     WGVSTRLVGALIMSHSDDDGLVLPPRVAPSHVVIWPVFRGDDTREAVLEAVNKLAAEL
                     RGQRFHERSIEVEVDLRDVSGGDKQWEWVKKGIPLRIEVGPRDVASGSAMVARRDRGP
                     KDKESIAFSDLPKRVPELLQAIQDGLLERAFAHRDANTRAIDTRAELEAFFTPRNADK
                     PEIHGGFALAHWCGSANCESALKEALKVTIRCIPTFGIARCAAYDSSTGPGRCVVCEG
                     ESQQRVVFAKSY"
     gene            12030..12833
                     /locus_tag="VFQ61_33565"
     CDS             12030..12833
                     /locus_tag="VFQ61_33565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity nickel-transport family protein"
                     /protein_id="HET9959484.1"
                     /translation="MSGVLSILVLGFLLGVRHATDVDHVVAVSALTSRGRSIGQALKI
                     GLAWGVGHALTVLMVGGAIVLLELRVPARLGLGLEFTVAAVLIVLGALNLSTAARELR
                     THRAAVPSVAGAVRPFTAAIGLGLHGFELVERVDGTHVGDPTRIDPHGSSEHTASRTT
                     LGSLGVGLAHGLAGSAAIALLVVSSLEQSNLALVYLAVFGVGTILGMSILTLLMALPV
                     AYATQRLGLRTANFACFAGILSLTLGLVLVYQIGFVHGLFTGNPSWHPG"
     gene            12871..13185
                     /locus_tag="VFQ61_33570"
     CDS             12871..13185
                     /locus_tag="VFQ61_33570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959485.1"
                     /translation="MTLHSCTRFTRWFSCAALLGCAAPAQETAPRSPTLDYPHPAPRT
                     SDGEVIGADGVDPTDKLRQGPTVGTPTAPGPGWKAEEGKLKPDPGGPGGSEPNPRNGP
                     PP"
     gene            complement(13327..14142)
                     /locus_tag="VFQ61_33575"
     CDS             complement(13327..14142)
                     /locus_tag="VFQ61_33575"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9959486.1"
                     /translation="MIKPAPEPLRAPIMIVCPQGELRLARGAVTIGRHSEADIRLDDV
                     LVSRLHARLSVLDDGAVLVEDLHSRNGVYVNGTRLSRGSERLREGDRLLIGTFELGVF
                     SARGEDAPGHAVKKLRMQRIDTAEPPPTDRAEGLILLARLAERLAREGKPDEAVRTLA
                     EQLHRVLLGAGSGLKPSQSLLEHAAEYAVKLFGWSQNRAWVEYVFELHLAAQCVPSEA
                     CIALLEATLASGRVVLDPNLVRYFDEFVAASAADSAHKQRLRALLAPCLVADH"
     gene            complement(14805..15242)
                     /locus_tag="VFQ61_33580"
     CDS             complement(14805..15242)
                     /locus_tag="VFQ61_33580"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET9959487.1"
                     /translation="MLVDEIKSRMFKAMKAGNTVEKEILRVAMGEITTDAARPGRTGS
                     DDEARAILRKLLKSNEETLAQPLEDAQRAALLEENRVLLDLLPKAPSLEDIQALLSSV
                     AGEIRAAGNDGQATGVAMKHLKASGASVDGKLVAQAVKALRTP"
     gene            15290..15928
                     /locus_tag="VFQ61_33585"
     CDS             15290..15928
                     /locus_tag="VFQ61_33585"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET9959488.1"
                     /translation="MSLHIGSIAPDFEQDSTSGRIRFHEWLGSSWGVLFSHPKDFTPV
                     CTTELGRAANLSAEFGKRNVKMLAVSVDDVESHKRWISDIDDTQGCRLDYPILGDSDR
                     KVAKAYDMIHEEANDTLTVRSVFFIDPAKKIRAIITYPASTGRNFQEILRVIDSLQLT
                     DNYKVATPVDWKDGDDVVIAPALQDPEDLKQRFPKGYRVLRPYLRLTPQPNR"
     gene            16098..16547
                     /locus_tag="VFQ61_33590"
     CDS             16098..16547
                     /locus_tag="VFQ61_33590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET9959489.1"
                     /translation="MKTKYALKALCHLAMASQDGPVLIADIAESENIPRKFLELILAE
                     LKQHGILQSRKGRGGGYQLAKTPENITVASVLRVLDGPIAPVPCLSRTAYQRCAECSD
                     EAVCGVRLVLREAHDASVHVLENTTLADMVKRVQAARGGATPLRYSI"
     gene            complement(16611..17108)
                     /locus_tag="VFQ61_33595"
     CDS             complement(16611..17108)
                     /locus_tag="VFQ61_33595"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9959490.1"
                     /translation="MQIANEKVITIDYTLTDEAGEVLDSSSDDGPLSYLHGFGNIVPG
                     LEAALEGKAKGDSVQVTVAPEEGYGKRDEALVQSLPRNRFPDGEIEVGMQFHAEAQGA
                     SRVLTVVAVDEKAVTIDANHPLAGRTLSFDVTVRDVRDATAEELEHGHVHDGDDHHHD
                     HDHHH"
     gene            17294..17869
                     /gene="msrA"
                     /locus_tag="VFQ61_33600"
     CDS             17294..17869
                     /gene="msrA"
                     /locus_tag="VFQ61_33600"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET9959491.1"
                     /translation="MTSNSTSSSTGNAPNAAGSHSNESPAAGLETAILAGGCFWGMQD
                     LLRKIPGVRETAVGYAGGSTPDPTYSTVKTGSTGHCEAVWIAFDPTQLSYRDLLEKWF
                     FRMHDPTTRNRQGNDLGSQYRSAIFFTSEEQRQAAEAAKEQVAKAGRWQRPIVTEIAP
                     AGPFYRAEGYHQDYLEKNPGGYTCHFLRDWT"
     gene            complement(18007..19002)
                     /gene="gap"
                     /locus_tag="VFQ61_33605"
     CDS             complement(18007..19002)
                     /gene="gap"
                     /locus_tag="VFQ61_33605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET9959492.1"
                     /translation="MAIKIGINGFGRIGRLAFRVAAARSDVEVVGINDLIDVKYMAYM
                     LKYDSTHGPFEGTVDVKDGKLIVNGKAIRVTSEKDPSNLKWGDVGGDYVIESTGLFLT
                     DETARTHIKAGAKKVVMSAPSKDETPMFVMGVNHTTYAGQEIVSNASCTTNCLAPIAK
                     VLNDNFKMVEGLMTTVHAVTATQKTVDGPSAKDWRGGRGAGQNIIPSSTGAAKAVGKV
                     IPALKGKLTGMSFRVPTPNVSVVDLTCRIEKGATYDEIKAAMKAASEGELKGVLGYTE
                     DAVVSTDFLTDPRTSIFDAEAGIMLNPNFVKVVSWYDNEWGYSNKLVDLLVHIAK"
     gene            complement(19253..>20771)
                     /locus_tag="VFQ61_33610"
     CDS             complement(19253..>20771)
                     /locus_tag="VFQ61_33610"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9959493.1"
                     /translation="VIPRSFLIMRLAAWLCLVAFGCSAPDLSRTYFSCKTDSDCLAGK
                     VCGLHNLQPACVDPSQDPIRLGMSGPLKGPSENLGTEMARGIRAQFEAVNRAGGIFGR
                     RLELDPRNDSYDPAQAIENTKSLLDIRNSVDDPDKPDERGANSVLALIGNVGTPTMLA
                     TAPIATKNHVVFFAPFTGAQAYLRDDTNSPYVFNYRASYYQETEAMVDYMKDNRNPRI
                     ITGNDSYRRILVFAQRDSYGDSGYAGLVNAYNRRVAPLPQPDPAQPSPSIAVIRYERE
                     NLPSVDPAVTAASAFLQDVLDTASNEQDLNSVAIVMVDTYQPGNKFIRGVKDWLNGSI
                     DRATRLDVAFMHVSFVGSDSLAQALSAVPESYVDVTDPTGMRRKYYADGVLVTQVVPS
                     YESKAPGVTQYRQDLKAYEDRATSFTSLEGYVAARLFVEALRQNGPTFTGDSLVKTLE
                     TKLTDLDIGIGTRLGFSSVDHQASDTVWGSILDAGAQFRVPFSWNPIDRIVVESN"
BASE COUNT         3879 a         6515 c         6310 g         4067 t
ORIGIN      
        1 ggcaccagcg cgagccaatc atcgaggctt ctcggggtct cgagtgggac atccgactcc
       61 gacagaacgc gctccgcgat gccgagcagc gcggcatgga tcgagcgtcg gacccaactg
      121 gcgtacggaa gggccggatc gacacgccgg ctgacgcggt gctcgacgaa tcgcttggcg
      181 agccgcgaga gcgaacgatg gttcaattct ttgattccga actcgtcgac cagccccacg
      241 tacccgagca cggctcccaa gctcggcagt tgacgagatt cgaccaactc tttgagggag
      301 ggtaacagcc gttccgcgtc gaactgactc tgaagcggca accggtcagc tacccactcc
      361 aacaactccg gcagaattcc agcgagcgct ggcgactcga caacctgaaa ctcagacgag
      421 gcgggaggct cgaagggcgc ggcgacgcac agtccgggcc gcggtgcgat cacgcgctgc
      481 gcctcgagcc cctgcgaatg ccctgtgagc tcgatgaaca gcgcgtcgga catcgctagc
      541 ttacccagca gctccgttgg cagctctgtc cctggttccc cgagctcgag aaactttgct
      601 cgaccgcgcg cctgcaacca ttgcccgacc aagcttcgcc ctgaaccgct cggcgccacc
      661 caccaaaccg gacgcgacga accgccgagc acttcggatg ggatgcccgg aggaagcgcc
      721 tcggaggaca aatccaaagc acgggcagtg gggagatcac gaagccgcct gatgcgcact
      781 tcgtcctgag ctggagcagg ttcacccaca gcgcggcgga ggtcatcgac cggtacccgc
      841 agcgcatccg cgagcaaccc ttggatattt ggccgctccg ctagccaaga gaggtcttgc
      901 ccccgatcca gtcggctcag cagcgtagcc aatgaacgag gccgaagccg cgaatccgcc
      961 ggccaaccct ccgctttttg cactgcatta gccaatgcac cgaagctccg aacaggtcgc
     1021 tcggagctcg ccattaggtc tcggagccag cccattcgaa tacctttgca ggatcataca
     1081 atcctgcatg caggatgcaa attcctgcat aactctcttt cgcggccttc aaatcacgcg
     1141 attcgagctt gtacgcgtct cctccccggc gttcgcaatg caggactacc tcgcgcactc
     1201 ctgcggtgtc aacgccggca cagcggagct tgaccgaacc cgtggtcgag cttgactcga
     1261 agtctggcct gcgagccggg attcacccct ttacttgcgc acggcagctt tgatcaggcg
     1321 cacgcgtgga gtttgaggcg cgccgcctct gttgcagcta atctcgcgcc ctcgctatga
     1381 gcggcattgc agaattcttg gtgtggtacc tggtgttcgt cttcagcacg acctgccacg
     1441 aagctgctca tgcctgggtc gcgcggcgcg gaggggacag caccgcgtat gcactcggcc
     1501 atgtcacgct ggatcccctt ccgcatattc gtcgagagcc gtttgggatg gttatcgtgc
     1561 cgatcctgtc gttctttcag ctggggtgga tcatgggatg ggcttccgtt cctttcgatc
     1621 cactctgggg agcgcgacac ccgaagcgct gggcgctcat gtccctagct gggccggcga
     1681 cgaattttct gctggcggca atggcgttcg ctgctttgcg tgggttgagc tcgatgaacg
     1741 tgctcagcct gacggacgct ggcggtgtga cggtcgggct ggtgcagctc ccccccggct
     1801 acgccttgaa ttcaccgctg ggtgcgttgg ggcttgggct cacggccttg ttgaagatga
     1861 acgttatctt ggggctctat aatttgctgc cggtgccacc gctggacggg gctgcggtcg
     1921 tggagggctt cgcgccgcgc gggcttcgat cgctctacga ttccattcgg cgcaatccga
     1981 tgctccaact gctgatgtta ttgggggctt ggtatgtgtt cccgacgctg ctcaaccctg
     2041 ttttccgcgt cgtgcttcgc tccctttatt cctgacctcg atttgcgcgc gccagctcgc
     2101 gcgcgacgaa gagcgaagcc aggctaatcg cctcaagctg gatctgcgag cacccgaacc
     2161 tcccggcggc gctcgagttt gctgagcgct gcggcttcaa tctgtcgcac tcgctcacgc
     2221 gagatgttca tcagcacgga tacatgctcc gctgatatgc ccgactggtc cgcaacatcc
     2281 agggcgcagc tgtgctgcat ctcccacact tcgagctcgg gaaagctcag ccgaatggtt
     2341 ccggtgcccg gagtcacgtc caggtacagg tgataccggc acccaacgta cgggcaaggg
     2401 cgcaggccat cgctgcaatc cgcgcggact ttcggccgtt cgagctgcag ttccagttct
     2461 tgcgcacgct cggcggcggc cactcgttcc tcggctccgc tcgcaaaccg caagcttttt
     2521 tgacgcaagt tcttgcggcg cgtggtgcca accacttgcg ccggctcggt tcgggagccg
     2581 tctctagagc caaccatcgg gcggtaacct gccaaaggtc ggcccgaatg gaaatggggt
     2641 tcttccccat tcgagttctc acttttgtgt cgttctgcgc gttcgacgct ttcgtgctca
     2701 acgcggcggg gggcgcaacg agcttcgcga tggcatcacc gtgcgacact cgagcacccc
     2761 cccgtcgacg ctcatcgcca ccaccgaggc cacatccgcg aacacgctga tgaacggcct
     2821 gccccactgc tcctccgcga aaacacgctg ccagacacca ccggtggcgc accatagctc
     2881 gcccgatgcg ccggcccact cgcgcgccaa aacgtcgatc gagacgctgg ctaggttggg
     2941 gcgctcgggg atcacgtctc ccgatacgct cccccgctcg agcctgagca cggcgccttc
     3001 ggcgcctacc gccagggcgg cttcgcgctc actgcgcgac gtgcaagcaa cccacgctcg
     3061 ggtcgcgggc gcgttcaact tgttcaattc ccaagacaat ggcgaataga gcgccgcaaa
     3121 accggttccg tgccgcgaac gcccgaccac gagccaatgc tcctcgtcga tttgcgataa
     3181 gccggctacg tacgccactt cgttcagggg cagaggcttg agccaacgtc ccgagaccgc
     3241 cgcccaaagc tctggcgtgc tttcatcacg taggcccacc atcaccacga cttcgcccgc
     3301 actgccgctc gcatccacga agctgaccgc aggatctttg cctcgaatca cgcggctgac
     3361 cccggcatga gaatactcgg ccaccagcgc ttcatcgccc ccgactagcc agcgtccggg
     3421 cgccacccgc cgcacgaatc tcaggccaag gcccggcggc agatccctgg tcggtgcggg
     3481 ttgccactgt gagccgttcc agtactcgag cccattggtg gtcgcggcca aacagtgccc
     3541 atcgccgtcc cagcccacac ttcgtacgat ccgtccgtcg ccgggcggat ggcgcacaat
     3601 ccagctcaat tgcggcaaaa ccgagtcctg ccccacgctc atcagagtcg cgcgctcttt
     3661 acgcggggca cgggtggctc gctccaacag caccgggacg aggccggcgg cgagctctcg
     3721 cggtgtttgc gggcgatggg cgggatcgac cgaagtggcg cgcgcaatca cggcatcgat
     3781 tgccgcgcaa gcatcgggat ccaggcgcag ctcatcgacc aaggccgcgg cttccgcgat
     3841 cgatcggcga cgcggctccg aagtgcgctg caccatctcg atggcattcg aggcttcgaa
     3901 taggtgttca cccgtgagca cataaaagac caaacaacca agactgaaga tgtcccccgc
     3961 aggaccgttg gtgttggatt gaatctgctc cgggcaaatg tagcccggcg tgcccatgac
     4021 cgcgggaccg aaggtatcgg ccatacccgc cggcttcgcc accccgaagt ccgagatttt
     4081 gaacaactcg gaatctccga agccacaaca aagcacgtta ttcggagtca gatcacggtg
     4141 aattaccccg accgcatgca cgtcggtcaa gccagaagcg acatgaccga ccacccgcgc
     4201 ggcgcgctct cgctcgaagg cgcgtcccgt gagcttgata ctatggcgaa cccggtcctc
     4261 gagcgtggtg ccttcaatcc ccccatccac gtattcgatc gctagccagg ggagctcgag
     4321 cgcggcccca cgatgctggt gaattacggt tccgccatcc atcagtcgaa ccacgaatgg
     4381 cgtcggcggc acctgctcgt tgagcctccc gagcgcgacc gattctttgc gcacgacggt
     4441 gaccgctttg ggaccgtgac cctgcacgat ttcgggaaga atgatcttca aaacggcggg
     4501 cgactcacca tgaggagaca cccgccacgc caggtaagcc gccgccgtcc ccccgagacc
     4561 caagcgccgt tcgagcacga agcgcacccc ggactcagtt tcggattcaa tcgcggcacc
     4621 gatgaatacg tccgtagcgg ggttggtcag cacgacgaaa gagcagccta actcagcgct
     4681 ccgcgagtga cgagggcttc gacccgacgc cccgagacta cgtccggccg cggcggcacg
     4741 tcagccgaag agcgcatttg tttcgattcg agccggtcca gcgctttgag cagaagtttg
     4801 ccaatccaag gtcgagaacc tgacggatgc cggcgtgctc ttgaggctga tattgacatc
     4861 cattttcaac ttccgtaccc ttcccgacac cgatggtacg cagacagaac cggctctcgg
     4921 ctccgttcac caatcttctt caggcgcttc aagctttgcc gctcatcccg acgcgcgccg
     4981 gagaagggct ggggctgatg gtcggattgc tcgcgttggc ctgctcgtcc gaagcggagc
     5041 ccgcggtcga acaagtcgtg gatcgaccga tcgtcgaaac cgtggatact ccgctgcccg
     5101 gaatcggccg cgagttcacc cgggatttca atgacggaga cctggcgttc agcacgccgc
     5161 tgcacgccgc ggacggcctt ggcccgctat atacgagggc ctcgtgcgcc gcctgccacg
     5221 acggaggtct gcgcggccca gggctcgttc agaagatgag tgtggtcgag gccgatggag
     5281 ttactcccgc ggctgatcaa ggcaaactcg catatggaca cacggtgcat ccattgacga
     5341 gtccggccgg ggaactcggg atcacgcctc cggttgacga ttcttcggtg aagatcagcg
     5401 aacgcttggg tccagccgtg attgggcgcg gctacatgga agccgtgctg gatagtgaaa
     5461 tcgagcgcgt ggccgaggag caagccgcgc gaacggacga gattcacggg cgggtcaatc
     5521 gcctcccgta tgcctccgag cccaatgtgg acaagcgctt ccacgactac aagaagggcg
     5581 atctggtgat cgggcgcttc gggctcaagg cgcgcatcgc taccttggac gactttaccg
     5641 ccgatgcctt tcaaggcgat atggggatca cgagcccgct ccgccccgac gagttcaaaa
     5701 acccgaacgc gctcgaagac cggaaaccag gggtcgatgt ctcgtatgcc agcgtgaaca
     5761 tgcgcgccaa ttacgtccgc gtgctcgaga ttccgacacg gcgtggtccc caggatgcgg
     5821 cgcggctctt ccaagacgtg cagtgtgccg cgtgtcacgt gccgagcctg cgcacgcgcg
     5881 cggactaccc gattgacgcc ctcgccgaca tcgatgctcc catttatacc gatctgttga
     5941 ttcacaacat gggtgaggat ctcgccgatg gtttgcccgc cgccgatgat gtggatggcc
     6001 aggctggctc gttcgactgg cgcacggcgc cactgatcgg tttgcgtttc aatcgcacct
     6061 ttttgcacga tggtcgcgcc aagagcgtag aagaagctat tttgatgcac cggggggtag
     6121 gctccgaagc caatgactcg gtggcaaggt tcgaggcgct gagcgagcgc gagcgcgcgc
     6181 tcctcgtcag cttcgtcgag agtctgtgaa cggatacgat atgagacact ggtcaatcat
     6241 tggagtactc ggcgtactct tggtttgtgg ctgcagcgat gactccgatt ctcccgcacc
     6301 gagttcggag tccagagtta cctccgaggt gcattcactg ctgctcacca atatcagcgc
     6361 tctgcatgaa tcggcgagtc agctgcaggc ggccgcgccg gcccacgcct gggatgccga
     6421 gcgggacgcg gaggccatct ctcaaatgac ggaggcctgg ctgaaggctc gcagtgagta
     6481 cgaacacatc gaaggcgcgg tggcgccttt gttcgcggag atcgacgtcg cgatcgacgc
     6541 acggtacgaa gacttcctgg agacgctcgg gaaagagggc gacggcaatt tgttcgatga
     6601 tgaaggcgtg accggtatgc acgccgtcga gcgcatcctg ttcgccaaag agatgccgga
     6661 ctcggtcgtt caactggagt cttcgttgcc gggctacacg cccgccgctt ggcccagcac
     6721 cgacgaggag gcagtcgcct tcaagaagca gctgtgcgct cgcctggtgg ccgataccca
     6781 agagctgctc acccagtgga agccgagccg cattgcggta gacggggcct tccgaggcct
     6841 gatcgggctc atgaacgagc aacgagagaa ggtcaacaaa gcctctaccc aagaagaaga
     6901 atcgcgctac gcacagcgca ccatggctga cctccgggac aacctagccg gtaccgagaa
     6961 ggtatacgga gtgttccaaa gttggatctt gtcaaaatct gggggcgccg aggtcaacga
     7021 gaaaattgaa cgacgcttcg gcgagctcaa gaccacctac gcctcggtca aagggaacgc
     7081 cttcccggcc gcacctgcga cctggagtgc ggaggatccg agcccggatg acctggcaac
     7141 cccgttcgga aagctgtata gcgccgtgca cgaagccgtc gatccgaacc gcgaagattc
     7201 gctggtgtcg gccatgaacg aggcgctcgt gttactaggc tacgaggagt tcgtcgaaga
     7261 atgatcactc acccgcttcg ggccatgacc gttttgcgca gcatcggctg cttgctcgcg
     7321 accctggtcg cggtgggttg tggctcgggc gggtccggag cggattcgac cgaagaggct
     7381 ggctcttttt tggccttcgc tggtgatttt caagggtttc gcgactggtc ttcgttcgac
     7441 gtcacgggtg aatcggccct gaccgaagtg catcaagggc aggtcatcat ctatgtcagc
     7501 gacctgcccc cgccaggtct cactcagttt ccaaccggaa cacggatcgt gaaagaagcc
     7561 atcctagaag gtcaaccgac ccagatcttc gcgatggcca aacgaggggg caacttcaat
     7621 tccggtgctc cgggttggga gtggttcgag ctcgaggatg tggcggaaaa gaacggcgtc
     7681 aaaatcaact ggcgtggttt cggtccgccc gcgggtgaga agtacgcggg cgacctggag
     7741 agcgaatgca atacttgcca tcgggcctgc ggcaatgact cggtgtgcac cactcacctc
     7801 gatttgagct cgttctgagt tcggtccggg ctcgcgcaaa cttggttggc cagtctcgac
     7861 ttctacctgg ctcactcaac gagccgcctc tttctcgagc tttgcttcat gagacttaga
     7921 ctttctttgc tggcggccgg tctcggcgtg tgcacttggt cagctagcgc aatcgccaac
     7981 ccccgacctt taccattcac ctatcagcac gagcagctgg cccaaggtga ctcggaaatc
     8041 gagcagtacg ccgactatac ggtggcgcgt gcgcgtgatg cgacgacggg agacccggta
     8101 tattacgggc taaccgcgtt tcagaccgaa ttcgaatacg gcatcctgcc gaatctggag
     8161 ctcgggcttt acttcacttt cgttccgacg acgaatgtcg gcttcgtgga cactcctcgc
     8221 ccgccgcaag gcaacgggct gaagcaacgt ctacgttatc aattcgcgcc aactggggtg
     8281 tggcccgtcg atttgtccgc ttatctcgag gtagtcgaga cggagcggga aatcgagctg
     8341 gaggcgcggc tgatcgtgca acgccgcttt ggccgactgc gcgccatcgt gaacttgagc
     8401 ggagaacgcg agttctatct caacggtgag cgggacttgg tcgcggcgcc ctcggcgggg
     8461 ctcacttacg agctgacgcc tgccatccaa ccgggaatcg agtggtggat gcgcgcggaa
     8521 tatcccgagc aaaacccgcc ctcaccccga ccattcgagc ttggaccgca ccaatacgtc
     8581 ggccccgtgc tcttgctctc tttcgggcgt ttgtggtgga ccacgggggc gtacgcgcgc
     8641 gtcagcgact tggaccacgg cattcgagcg ggcgagtcgt acggtaagtt ctgggcccgc
     8701 accatcgttg gcttcgggct ctagcccttt ccgcatgcgc tgggcgcgcc cagctttcga
     8761 cgcacgttac gacgcctgct gctgcggcag gcgaatgacg aagcacgttg gatattcgtc
     8821 ggtttgaatg acctcgatat cgccgccatg ggcctctatc gctcgcttgc agaagtagag
     8881 ccccagcccg gcgttgcccg tggtgcgtac cccagcgttg gagccacgcg taaacttctc
     8941 gaagatcagc ttgcgttcag cctcggggat ggccgggccg ctgttcgcga ccacgatctg
     9001 aatttcgttt tggacttccg ccccaatgaa gacacgtcca ctctccgggg tgtagcgcaa
     9061 ggagttgtcc agtatgtttt ccagcacgcg ctggatcaaa ctcggatcga cttgcacgaa
     9121 cgcggcgttc tccggagact cgacgagcgc gatcttgcga ttttctgcgg cgcgagcgaa
     9181 gccgttcgtg acgctgcgta gaatgggtcc aacctgcacc ttgcgcggct tcacggggaa
     9241 cgtgcctcgt tcgagctgcg acaccgcgat cagatcgttg atcatgctgc gcagcctgcc
     9301 cgcgctgccg agcacgtctt ccagcgcgcg cagcaaggcc agctccttgc gctggagctg
     9361 ctcgtagacc cactccgtgt tcgcgacgat gccagtcagc gggcccttca aatcgtgcac
     9421 gatgaactcg gtcagttccc gctgttcacg ctgcgctcgc tcgagttcgg cattgcggat
     9481 caacagcaga tctcgcgatt gcttgaggcg cagcagggtg ttggcccgcg ctagcaagac
     9541 tcgagcatcc agcggcttct cgagataatc gtcggctccc gcttccaaac cccgcaagcg
     9601 gtgctcgcgt tcggcgtgcc ccgtcagcaa gatgacgggg gtatcatagc gcgtagcgtg
     9661 ggagcgaatt cgcctgagca cctcgatccc gtctagcccc ggcatggcta ggtccaggat
     9721 caccaagtcc ggttctgatt cctcgaagcg tcgtaccgcg gtatggccat cttcggccgt
     9781 gctgatgctg gcttccaagg gctccagaca ggcggcgagc gcgctcaagt tcaaggcttc
     9841 atcgtcgacc agaagcaact cgggtcgcca gttcatgctc gcgtcgaagg ccaaggctga
     9901 agcgctcacg atagccgttc tcgcgtttcc caccaatcga ttcgagtctc acacgctgta
     9961 tgtccgcaga cgagcgtgca tgaccgtgtg tgtcttggca ggccgagacg tgaatcggta
    10021 cgtctttcgc gcgcttgacg cgatgatttc cgccctttca ccatccacga ggataccgcc
    10081 gcgcgtcagc cccctgacat gaatgagctc atggcggggc gagcgattca ctaggtccgg
    10141 acccggcact cgatgggatt gtaagagcag ctcgaaccgc gtggttgggc ttccaatcgg
    10201 gtcagacccg ccggacacgg cgagcctgac ctcttgagcg agtgttcggc gaatttcgtc
    10261 agtaactctt ggcgaatacg acgcgctgct gactttcgcc ttcgcagacc acacaacggc
    10321 cgggccccgt cgagctatcg taggccgcgc aacgcgcgat tccgaacgtc gggatgcagc
    10381 gaatcgtcac cttcagcgct tccttgagag cgctctcgca gttcgccgag ccgcaccagt
    10441 gcgccaacgc gaagccacca tgaatctcgg gcttgtccgc atttcgaggg gtgaagaagg
    10501 cctccagctc ggcccgagtg tcgatggcac gagtgttcgc gtcccggtga gcaaatgccc
    10561 gttcgagcag gccgtcctga atggcctgta agagttccgg aacgcgcttc ggcaagtcgg
    10621 agaaggcgat cgactctttg tccttcggtc cgcgatctcg tcgcgcgacc atggcactgc
    10681 cggatgcgac gtcacgaggg ccaacttcga tgcgcagcgg aatgcccttc ttgacccact
    10741 cccactgctt gtctccgccg ctgacatcac gcagatcgac ctccacctcg atacttcgtt
    10801 catggaagcg ctgaccgcgc agctctgcgg ccaatttgtt tacggcttcg agcacggcct
    10861 cgcgcgtatc gtcgccgcgg aaaaccggcc aaatcaccac atgggagggc gctactcgcg
    10921 gcggtagcac gagcccgtcg tcgtcgctgt gactcatgat cagcgccccc accaaacgcg
    10981 tagacacgcc ccacgacgtg gtccaggcca agcgttgctg gcctgattta tccaagaacc
    11041 ggatcccgct cgcctccgcg aatcgctgac ccaagaagtg agaagtgccg gcttgtaggg
    11101 ctttgcggtc ctgcatcatc gcttcgatcg cgaaagtgtc gaccgctccg gggaagcgct
    11161 cgctgtcgct cttggggcct cggatcaccg gcatcgccag gtagttttcg gcgaaggtcg
    11221 cgtacacgtc caacatctgt cgggtctcgc ggcgcgcgtc ttcttcggtt tcatgcgcgg
    11281 tgtgcccttc ctgccaaagg aactccgcgg tgcgcaggaa cagccgagtg cggagttccc
    11341 aacggaccac attcgcccat tggttgatca gcaacggcag gtctcggtag ctcttcaccc
    11401 actgcgcgaa ggccgcgccg atgatcgtct ccgacgtcgg gcgaaccacg agcgcttcgt
    11461 cgagttctgc ctccgggtcc ggaatcagcc cgccgtcttt gccagccacg agccggtggt
    11521 gcgtgacgac cgcgcactct tttgcgaagc cctcgacgtg ctcggcttcc ttttcgagaa
    11581 aagatttcgg gatgaagagc gggaaatagg cgttcttgtg accggttgcc ttgaacatcc
    11641 cgtccaagac acgctgaatg ttttcccaga tcgcatagcc ccacggcttg atcaccatgc
    11701 agccgcgcac cggcgaggtc tcggccagat cggcggcgcg caccacctct tggtaccatt
    11761 gcgcgtagtc ttcagtgcgg gtgggggaaa ttgcggtctt agctgaggga tttttggcca
    11821 cggtcagggc tccaggaagg ccccgggtat actcgtttgg ggtcggggca caacccgcac
    11881 caaaactaac ccgaaactca tgttccggcc gtgcggagag ccttgaggtg caatcgtcga
    11941 acgacgggct tcgtagctcg ggtgatcgct gaccaggggt cgagcaaagg gtgttactcc
    12001 tgctcgaatt cgtgctacga gcagaacgag tgagcggcgt tctctcaatc ttggtgctcg
    12061 gcttcctact cggggtaagg catgccaccg acgtggatca cgtggttgcc gtgagcgcgc
    12121 tcacctcgcg cgggcgctcc atcgggcagg cactcaagat cggactggca tggggagttg
    12181 ggcacgcgct taccgtcttg atggttggtg gcgcgattgt gttactcgag ctgcgcgtgc
    12241 cggcgcggct cggcttggga ttggagttca cggtcgcggc ggtgctcatc gtgctgggcg
    12301 cgttgaattt gagcaccgcg gcgcgtgagc ttcgaacaca ccgggctgcc gtaccgagcg
    12361 tcgcaggggc ggttcgccct tttactgcgg caatcgggct tggactgcat ggctttgagc
    12421 tggtcgaaag agtcgacgga acgcacgtgg gtgaccccac ccggattgat ccccatggct
    12481 cgtcggaaca taccgcatcg cgcaccacgc tgggctcgct cggcgtgggc ctagcgcatg
    12541 gcttggctgg ctcagccgca attgcgctgc tcgtcgtgag ctcgctcgag caatccaact
    12601 tggcgttggt ttacctcgcg gtgtttgggg tgggaaccat cctagggatg tcgatcctga
    12661 cgctgctgat ggccttaccg gttgcgtatg cgacgcagcg tttgggcctc cgcaccgcga
    12721 actttgcgtg cttcgccggg attttgagcc tcaccttggg gctcgtgctc gtctaccaaa
    12781 tcggcttcgt acacggccta ttcaccggca atcctagctg gcaccccggt taggatttgt
    12841 tccggctcag catttgctac gctgaagggc gtgacccttc actcctgcac tcgcttcacg
    12901 cgctggttct cgtgtgccgc gctactgggc tgcgccgcgc cggcacagga gaccgcccca
    12961 cgctcaccaa cgctcgatta cccccatcca gctccgcgaa cttcggacgg agaagtgatc
    13021 ggcgcagacg gcgtggatcc cacggacaag ctaagacaag gcccgaccgt cggcactccc
    13081 actgccccgg gtccagggtg gaaagctgag gagggcaagc tcaagcccga tccagggggc
    13141 cccggcggct ccgaacccaa tccccggaac ggaccgcccc cctgaacggg cgcggcatcg
    13201 gcgcagattc tgggctgccc aacggcctgc ggctcgagtc gtccactttg catgagccac
    13261 gtttacacga gcaatctgct ctgatctggc aatctgcttc gctcggaaaa tcctgcgtgg
    13321 ttcagctcag tggtcggcta cgaggcaggg cgccaacaat gcgcgcaagc gctgcttgtg
    13381 cgcagaatcg gccgcactcg cggccacgaa ctcgtcgaag taacggacga gattcggatc
    13441 gaggactacc cgccctgacg cgagtgttgc ctccaatagc gcaatgcaag cttcactcgg
    13501 gacgcattgc gccgccaaat ggagctcgaa tacgtactcg acccaagcgc ggttctggga
    13561 ccacccaaac agctttacgg cgtactcggc agcgtgctcg agtagcgact ggctgggctt
    13621 caaaccagaa ccagcgccga gcaacactcg atgcagttgc tccgccagcg tgcggaccgc
    13681 ttcatcgggc ttaccttcgc gcgccagtcg ctccgccaac cgcgccagca aaatcaggcc
    13741 ttcggcgcga tcggtcggcg ggggctcggc ggtatcgatc cgttgcatgc gcagtttctt
    13801 gactgcgtga cccggggcat cttcaccgcg cgccgaaaac acgcccaact cgaaggtgcc
    13861 gatcagcaag cggtcccctt ctcggaggcg ctccgagccg cgagacagac gcgtgccgtt
    13921 gacgtaaacc ccgttgcggc tgtgcagatc ctcgacgagc accgctccat cgtccaacac
    13981 gctcaaccgg gcgtgaagcc tggataccag cacgtcatcc aagcgaatat ccgcttccga
    14041 gtgccggcca atcgtcactg caccccgggc gagccgcagc tcaccttgtg gacagacgat
    14101 catgatcggt gcccgcaacg gttcgggcgc aggcttgatc atccgctcta cgatacgagc
    14161 gagacgagag ggtgttgcac gcggcttgtc cggcattgaa aagtgccctg atcgtcaaat
    14221 catagaagat gatttgtccc tggggaatac cgagtttgca ttgcgctgag ttttaccggc
    14281 ggcggtcact cgcaactggc gctccaatgc tgcgctgaga agatcgagga tgctcgagtc
    14341 gtttttgggg tcgacggctt ggagcggatc tcccccgaag tcgaggttta ccaggagttt
    14401 cttaaacagc tctcgatcca gaaacagccc taagaccacg tttgcttacc gttttggtga
    14461 cggctggcct tctcccgatt cttctaggtg ctttggacgt gtgctccggc ctgcctgaaa
    14521 gacccggctt tatagcggaa tgacagataa ttgacggggg gcggcttgct cgtgcacaca
    14581 tgggtacggc gccgcactga tgcgccgtac cgcgctgaga attcccgatg atctctcgat
    14641 gctcgacgga ccgcgcccga accgtaacat cgagccttac gtattcgtgc gcatgtattc
    14701 gtgcgcacgt attcatgcgc gcgtattcat gcgcgcgtat tcacgcgcac gtactcacgg
    14761 gcacggctct gccacgagtt gaagcttcag ttccgctcag tgcgtcaggg ggttctgagg
    14821 gccttaacgg cttgagcaac gagcttgccg tcaacgctcg cccccgatgc tttgagatgc
    14881 ttcattgcca cgcctgtggc ttgaccgtca ttaccggcgg cacgaatttc tcctgccacg
    14941 gagctgagca gcgcttgaat atcttctagc gatggcgcct tcggcagtag gtcgagcaga
    15001 actcggttct cttcgagtag tgcagcgcgt tgagcgtcct cgagcggctg agctagcgtt
    15061 tcctcattgg acttcaaaag cttgcggaga atcgcccgag cttcgtcgtc gctccccgtt
    15121 cggcccggtc gcgcagcatc ggtcgtgatt tcacccatcg cgacgcgaag aatctccttc
    15181 tccaccgtgt tgccggcttt catcgctttg aacattcgag acttgatctc atcgacgagc
    15241 atgctgtgtt ctgtgcctcc acccgtgtat tgcctcagga ggtcaaggaa tgtctctaca
    15301 tattggcagc attgcgcctg atttcgaaca ggactcgacc agcggccgca tccgattcca
    15361 cgaatggttg ggcagtagct ggggcgtgct gttctcccat cccaaggact tcacgccggt
    15421 ctgcacgacg gagctcggac gcgcagcgaa ccttagcgcg gagttcggaa aacgtaacgt
    15481 gaagatgctc gccgtgagcg tggatgacgt ggagagccac aagcgctgga tctcagatat
    15541 cgacgacacc caaggttgcc ggctcgacta tcccattctg ggcgactccg accggaaggt
    15601 ggccaaggcg tacgacatga tccacgagga agcgaacgat acgctgacgg tccgatcggt
    15661 gttcttcatc gatcccgcca agaagatccg cgccatcatc acttatccag cgagcaccgg
    15721 ccgcaatttc caggagattc tgcgggtgat cgattcgctc cagctgaccg ataactacaa
    15781 ggtcgcgacc ccggtagatt ggaaggatgg ggacgacgtc gtgattgcgc ccgcgctgca
    15841 agaccccgaa gacctgaagc agcgcttccc gaaaggctac cgggtgctcc gtccctatct
    15901 ccggctgacc cctcagccga accgctaacg gccccgtccg taatggctaa cgggcccaag
    15961 ctcacgaggc tggcctgtta gccggagtgg cgccctcggt catctcgaga gcgagacgga
    16021 ccatagagca aaatccctgc gcggtcggcg aatcagcggc gtggcgtctg cgctatacgc
    16081 tagcggcgat gatcacgatg aagacgaagt acgcgctcaa ggccctgtgc catctcgcga
    16141 tggcctcaca ggacgggcct gtcttgatcg cggacatcgc ggaaagtgag aacatcccgc
    16201 gcaagtttct cgagctcatc ttggccgagc ttaagcaaca cggcatcctt cagagtcgca
    16261 aggggcgcgg cggtggttac cagctcgcga agacgcccga gaacatcacc gtcgcctcgg
    16321 tgcttcgagt actggatggc ccgatcgcac ccgtgccgtg tttgagccga accgcgtatc
    16381 aacgctgcgc agagtgttcc gatgaagccg tgtgcggagt tcggctggtg ttgcgcgagg
    16441 cacatgacgc ttcggtgcac gtgctcgaga acaccactct cgcagacatg gtcaaaagag
    16501 tccaagcggc gcgcggtgga gcgacgccgc ttcgatactc catttagggc agctgaaagt
    16561 tggcgcgccg ctcaatcgat acgtgagcta ccggtgcgcc cgacgaacac tcagtgatga
    16621 tggtcgtggt cgtggtgatg atcgtcgccg tcgtgcacat ggccatgctc gagctcctcg
    16681 gccgtcgcat cacgcacgtc tcgaacggtg acgtcgaatg agagcgtccg cccggcgagg
    16741 ggatgattgg cgtcgatcgt cacggctttt tcgtctaccg ccaccactgt gagcactcgc
    16801 gacgcgccct gcgcctctgc atgaaactgc attccaacct cgatctcgcc gtccgggaat
    16861 cggttacgcg gaagcgactg gaccagcgcc tcgtcgcgtt ttccgtaacc ttcttcgggg
    16921 gccaccgtaa cctgcaccga gtcgcccttt gccttgcctt ccagagccgc ctcgagcccg
    16981 ggcacgatgt tgccaaaccc atgaaggtag ctgagcggtc cgtcatcaga ggagctgtcg
    17041 agcacctccc ccgcctcgtc cgtcagtgtg taatcgatag tgatgacctt ttcgttcgcg
    17101 atctgcatga ggggggctcc aggctccacc gggtcttccc gggtcggagt ggcgcgaaga
    17161 ctacgctcgg gagccaggct gcgcacgaca ttttcggcac gcgggcgctg tgtctggtgc
    17221 gcgggttgcg gcacgggtgg ggcgagcgcg ctcgctcgtt gacacgcagc gggaaagagt
    17281 caggaacatc ggaatgacct cgaactccac ctctagttcc acgggtaacg cgccgaatgc
    17341 cgccggatcc cactcgaacg aatcccctgc ggccggctta gagacggcta tcttggcggg
    17401 tggctgcttc tggggcatgc aggatttgtt gcgaaaaatc cccggtgtac gggaaaccgc
    17461 ggtgggctac gcaggcggtt cgacaccaga cccgacttat tcgacggtga agaccggcag
    17521 cacgggccac tgcgaagccg tttggatcgc attcgatccg acgcagctca gctaccgcga
    17581 cctactcgag aagtggttct tccggatgca cgatccaacc acgcggaacc gtcagggcaa
    17641 cgacttgggc tcgcagtacc gctctgccat cttcttcacg agcgaggagc agcggcaggc
    17701 tgctgaggca gccaaagaac aggtggcgaa ggccggccgt tggcaacgtc cgatcgtgac
    17761 cgagatcgca cctgccggac ctttctaccg agcagaaggc tatcaccagg actatctcga
    17821 gaagaatcct ggcgggtaca cgtgccactt tctgcgcgat tggacttagt ttagcggtgc
    17881 tgctccgaat ctgacgagac gaacaaaaca gctgcggccg gcccggaaca atgccggagc
    17941 cggccgcgag ttgctcgcgc gtgactcgag aatcacagag atcgagtcac ttcgcggttt
    18001 ctagagtcac ttcgcgatgt gaaccagcag gtcgacgagc ttgttcgagt aaccccactc
    18061 gttgtcgtac caggagacga ccttgacgaa gttggggttc agcatgatgc ccgcttccgc
    18121 gtcgaaaatg gaggtgcgcg gatcggtgag gaagtccgtc gaaacgacgg cgtcttcggt
    18181 gtagccgagc acgcctttga gctcaccttc ggaggcggcc ttcatggcgg ccttgatttc
    18241 gtcgtaagtc gcgccctttt cgatgcgaca ggtcaaatcg acgaccgaca cgttcggcgt
    18301 ggggacgcgg aacgacatac ccgtcaactt gcccttcaaa gcagggatga ctttgccgac
    18361 cgctttggcc gcgcccgtcg aggacgggat gatgttctgg cctgcgccac gaccaccacg
    18421 ccagtccttg gcagacggac cgtcgacggt cttctgggtc gcggtgacgg cgtgcacggt
    18481 cgtcatgaga ccttccacca tcttgaaatt gtcgttcagg accttggcga tgggcgccaa
    18541 gcagttggtg gtgcaggagg cgttcgagac gatctcttga cccgcgtacg tcgtgtggtt
    18601 cacgcccatc acgaacatgg gcgtctcgtc ctttgaaggc gcgctcatca cgaccttctt
    18661 cgcgccagct ttgatgtgcg tacgcgcggt ctcgtcggtc aggaacagac cggtagactc
    18721 gatgacgtag tcaccgccga cgtcgcccca cttgaggttg gaggggtctt tttcggaggt
    18781 cacgcggatc gccttgccat tgacgatcag cttgccgtcc ttgacgtcca cggtgccttc
    18841 gaacggacca tgcgtcgagt cgtacttgag catgtacgcc atgtacttga cgtcgatgag
    18901 atcgttgatg ccgacgactt cgacgtcgct gcgggctgcc gcaacgcgga atgccagtcg
    18961 accaatacgt ccgaaaccgt tgataccgat cttgattgcc atagggcgat agcctttctt
    19021 tctcagccgc gatccggccc ggccggatcg tgtagcgccg cgctgctgcg tagtccgagg
    19081 tgcgcactca acgcaagcca tcttctgcca ggcccagagc tcggttgcag ccgttttttt
    19141 tgctatcccg gcgcaaactt gccggccagg cgcccagaca acagccctgc ggcgcgcagc
    19201 gtcatgccgc aacgcagagc tcgcactgac acatgccagg ctcgaacgcg cgtcagttcg
    19261 actcgaccac gatgcgatcg atgggattcc agctgaaagg caccctaaac tgcgcgcctg
    19321 cgtccaggat gcttccccag acggtatcgg acgcttgatg gtccaccgag ctgaagccga
    19381 gccgcgttcc gattccgata tccaagtccg tcagcttggt ttccagagtt ttcacgagcg
    19441 agtcgccggt gaacgtcgga ccgttctggc gcagggcctc gacgaacagc cgagccgcga
    19501 cataaccctc gagcgaagtg aagctggtcg cgcgatcttc gtaagccttc aagtcctggc
    19561 ggtactgtgt aactccggga gccttggatt cataactggg aacgacttgc gtcacgagca
    19621 ctccgtcagc gtaatacttt ctgcgcatgc ccgttgggtc ggtgacgtcc acgtaagact
    19681 cgggaacggc tgatagagcc tgcgccagcg agtcgctgcc cacgaaggag acatgcatga
    19741 acgcgacgtc gagccgcgtc gcgcgatcga tgctgccgtt gagccagtcc ttcacgccgc
    19801 ggatgaactt gttgccaggc tggtacgtgt cgaccatgac gatcgccacc gaattcaggt
    19861 cctgttcgtt cgaagctgtg tccagcacgt cttgcaggaa cgcgctagcg gccgtgaccg
    19921 ccgggtcgac gctcggcaag ttttctcgct cgtagcggat cacggcaatg gacggcgagg
    19981 gctgcgccgg gtcgggttga ggcaacggag cgacacgacg attgtaggcg ttcaccaagc
    20041 ccgcgtagcc tgaatcgcca tacgaatcgc gctgagcgaa gaccagaatc cgacggtagg
    20101 aatcgttgcc cgtgatgatg cgcgggttcc gattgtcctt catgtagtcc accatcgctt
    20161 cggtttcttg gtagtaactc gcgcgatagt tgaacacgta aggagagttc gtgtcgtcac
    20221 gaagataggc ttgagcgccg gtaaagggcg cgaagaagac gacgtgattc ttcgtcgcga
    20281 tcggggcggt ggccagcatg gtgggcgtac ccacattgcc aatcaaggcg agcacgctgt
    20341 tggcccctcg ctcgtccggc ttgtcggggt catcgacgct gttacgaata tcgagcagcg
    20401 atttggtgtt ctcgatggct tgcgcgggat cgtacgagtc gttgcgcggg tcgagctcca
    20461 accggcggcc aaagatgccg cccgcgcgat tgacggcctc gaactgcgcg cgaatgccgc
    20521 gcgccatctc ggtgccgagg ttctcgctcg gccccttgag tgggccgctc atgcccaggc
    20581 ggatcggatc ctgtgatgga tcgacgcagg ccggttgcag gttgtgtagc ccacatacct
    20641 taccggcgag acaatcagaa tccgtcttgc agctgaaata ggtgcgcgac aaatctggag
    20701 ccgaacaacc gaacgccact aggcacagcc aagctgcgag tcgcatgatc agaaagctcc
    20761 ttggaatgac a
//
LOCUS       DASTKU010000948         4578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_631349, whole genome shotgun sequence.
ACCESSION   DASTKU010000948 DASTKU010000000
VERSION     DASTKU010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631349"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..2365
                     /locus_tag="VFQ61_33615"
     CDS             449..2365
                     /locus_tag="VFQ61_33615"
                     /inference="COORDINATES: protein motif:HMM:NF038117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor I family protein"
                     /protein_id="HET9959494.1"
                     /translation="MFRTRHGQLQRSARSGVIVSWSLALLAAACTGETGPVGPMGLQG
                     PEGKQGPQGEPGVDGKDGVDGTDGKDGVNGTDGKDGVDGTDGADGEDGKDGTNGKDGV
                     DGSDATPRPVGLSLSLLGRHSTGLFDEGSQEIISYDSKTGRLFTMNAAAQTVDVLNIS
                     DPQHPARVGTIDVTQADARALGDITSVSARAGLVAVAVGANPRTDPGLVLFYDAQTLA
                     LRGKVEVGALPDMITFTPDGTKIVVAIEGEPNDDYTVDPEGAVGIIGVPDDFTGPIQY
                     TTADFHAFDTKAAELRAKGVRIFGPKHENDTWTPSGATVSQDLEPEYVAVSADSKKAW
                     VTLQENNAIAVVNLETASVEEIRPLGFKNFNLLGNEFDPSDKDKGAYLRAWPVYGMYM
                     PDAVARFEVAGETYLVTANEGDVRAFGDFNDAVRVSTLELDPVAFPNASELKADAALG
                     RLQVTPNLGDSNADHKYERLYAFGGRSFSIWKADTGQLIYDSGNELELITAKRLGDGF
                     NSSHAASGGDARSDDKGPEPEGVTVATIEGSTFAFITLERPGGVMIYDVTLPESPRFV
                     SYVNTRDITQTYGGEGFVWDEAAQQRIGDLGPEASVYIPSTEPGEPGILALGNEVSGT
                     TVLFRVTPLYGVLP"
     gene            complement(2373..3476)
                     /locus_tag="VFQ61_33620"
     CDS             complement(2373..3476)
                     /locus_tag="VFQ61_33620"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9959495.1"
                     /translation="MAEPVDAVVIGAGVVGLAIARTLALASRDVLVVEAESAMGTHTS
                     SRNSEVIHAGIYYPTGSLKASLCVTGKAALYAYCAEHEVEHVRIGKVIVATREQEIPV
                     LDRLLTQARENGVHDLVPLSADEVHELEPKVVAVRGLLSPSTGIIDSHGLMAALRREA
                     EAHGAQVVTQSPVVAGRVTDQGIELEIGGPEPISVLAKSVINSAGLRAQEVSRSILGL
                     PRESIPPQHFAKGHYFVLPGKSPFSRLVYPVPVPGGLGIHVTLDLARQTRFGPDVSWL
                     SGVDYAFDAGRAEQFYAAIRTYYPDLREGTLEPGYTGIRPKLGPKGSEAADFMIQGPA
                     EHGVPGLVALYGIESPGLTACLAIAERVSALLQ"
     gene            complement(3476..4174)
                     /locus_tag="VFQ61_33625"
     CDS             complement(3476..4174)
                     /locus_tag="VFQ61_33625"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HET9959496.1"
                     /translation="MKSFFQFLSKPLPSVVRGGRFGRSAASTRACSRACHAVLIGALV
                     AGAAFETGCASDGQYVWFTRVPRSTLAPPNEYIIGIGDSISIEVYQQKDMSLKGRVRR
                     DGRLAMPLLNEVVAAGKTPAALAKELEGRLKQYLQEPRVTVNVEEATPISVTILGEVK
                     SGGTLTLEPPASMLQAMAKAGGLSEYGDHDRIFLLRKVPQFQRIRFTYDAIMNNESGA
                     AMFPLQSGDVIVVQ"
     gene            4310..>4578
                     /locus_tag="VFQ61_33630"
     CDS             4310..>4578
                     /locus_tag="VFQ61_33630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959497.1"
                     /translation="MKPRVGFVVEQALGHVAYGQSLRKALAGREDLEIVWLEIPYGFE
                     GMGKIPLVGRNWTLRGSARAFRAIQQAHAKTPLDALFLHTQTISV"
BASE COUNT          898 a         1408 c         1454 g          818 t
ORIGIN      
        1 gcgggtggtc gtgctgcgcg gcgtgaccat cggtgagggc agcgtggtgg gtgcggcgag
       61 cgttgtcgcg cgcgacgtgc caccgcgaac gctagtcgca ggtgtgcccg cgcgtgtgat
      121 caagaagctg tagtcaagca aaccagtctt gggaccatcc gggctggcgg attcgggcgt
      181 acctgtctct ggtctagagc ggtgtttcta gacttcgaat tctacgttcg tatctctaga
      241 gcggtggttc ggtagggaac tgggatcggt ggcgcgcggt ggagcgacgg caatcgtgcg
      301 gtttgcctgc agccctcgac catcgattga cgacaatttg cctatttatc gctgaatcgt
      361 ttctttcgac actcaagagt cactcgccga gtcgacagac tgagcgaaga aacggggcga
      421 gcagggggat ctaaaacgag gaatcaccat gtttagaact cgtcacggtc aattgcagcg
      481 ctccgccagg tccggggtga tcgtaagttg gagtctggcc ttgttggccg cagcatgcac
      541 cggtgaaact ggtcctgtgg gcccgatggg tctgcaaggc cctgaaggca agcaaggccc
      601 gcaaggtgaa cccggggtcg acggcaaaga cggagtggat ggcaccgacg gcaaagacgg
      661 agtgaatggc accgacggca aagacggagt ggatggcacc gacggtgcgg atggcgaaga
      721 cggaaaagac gggacaaacg gcaaagacgg cgtcgatggc tccgacgcaa ccccgcgccc
      781 ggtgggtctc agcctgtccc tgctgggccg tcactccacc ggactattcg acgagggcag
      841 ccaagaaatc atcagctacg attccaagac cggtcgcctg ttcacgatga acgccgccgc
      901 tcagacggta gatgttctaa acatcagcga tcctcagcac cccgcgcgcg tgggcacgat
      961 cgacgtcacg caagcagacg cacgcgcgct cggtgacatc acgagtgtct ccgcgcgggc
     1021 aggtctggtt gcggttgcgg tgggcgcgaa tccgcgcacc gaccccggtt tggtgctgtt
     1081 ctatgacgcg cagacgctag ccttgcgcgg caaggttgag gtcggcgcac tgcccgacat
     1141 gatcacgttc acgccggatg gcacgaagat cgtggtcgcg atcgagggcg aacccaacga
     1201 cgactacacc gtggatcccg aaggtgcagt ggggatcatc ggcgtgccgg atgacttcac
     1261 gggtccgatc cagtacacga ccgcagactt tcacgccttc gacaccaagg cggcggagct
     1321 gcgcgccaag ggcgtgcgca tcttcggccc caagcatgaa aacgacactt ggactccgtc
     1381 aggcgcgacc gtgtcgcagg atctcgagcc cgagtacgtc gcggtctccg ccgatagcaa
     1441 gaaagcttgg gtaaccctgc aggagaacaa cgccatcgcg gtcgtgaacc tggagacagc
     1501 cagcgtcgaa gaaatccgcc cgctgggctt caagaacttc aacctcctcg gcaacgagtt
     1561 cgacccgagc gacaaggaca agggcgccta cctgagggct tggcccgtct acggcatgta
     1621 catgcccgat gccgtggcgc gtttcgaagt cgccggcgag acctacttgg ttaccgcgaa
     1681 cgagggcgac gtccgcgctt tcggagactt caacgacgcc gtacgcgtct caaccctcga
     1741 gctcgatccc gtcgcgtttc cgaacgcgag cgagctcaag gcggacgccg cgctcggacg
     1801 tcttcaagtc acgcccaact tgggcgacag caacgccgat cacaagtacg agcggctcta
     1861 cgcattcggg gggcgctcgt tttcgatttg gaaagcagac accgggcagc tcatttacga
     1921 cagcggcaac gaactcgagc tcatcacggc caaacgcttg ggtgatggtt tcaactcgag
     1981 ccacgccgca agcggtgggg acgcccgctc cgacgacaaa ggccccgagc cagaaggggt
     2041 caccgtggcg acgatcgaag gcagcacctt cgcgttcatc actctcgagc gtcccggggg
     2101 cgtgatgatc tacgacgtga ctttgcccga gagtccgcgc ttcgtgagct acgtaaacac
     2161 gcgtgacatc acgcagacgt acggcggaga gggtttcgtg tgggacgagg ccgctcagca
     2221 gcggatcggc gacttgggtc ccgaagcaag cgtgtatatc ccctccaccg agcccggcga
     2281 acctggcatt ttggcgttgg gcaacgaggt cagcgggacg accgtgctct ttcgtgtcac
     2341 tccgctgtac ggtgttttgc cttgaattta gttcactgaa gcagcgctga gacgcgctct
     2401 gcaattgcca gacatgcggt caagcccggg gactcgatgc catacagcgc gacgagcccc
     2461 gggacgccgt gttctgccgg accctgaatc atgaagtcgg cggcctcgct gcctttgggg
     2521 cccagcttcg gtcggatccc cgtgtagccc ggctcgaggg tgccctcgcg aaggtctgga
     2581 taataggtcc tgatcgcggc atagaactgc tcggcgcgcc cggcgtcgaa cgcgtagtcc
     2641 acgcccgaga gccaggagac atcgggacca aagcgcgtct gccgagcgag gtccaacgtc
     2701 acgtgaatgc cgagcccgcc cggtaccggc accggataaa ccaagcgtga aaacggtgat
     2761 ttgcccggca agacgaagta gtggcccttg gcgaaatgtt gcggcggaat gctctctctg
     2821 ggcaggccga ggatgctgcg cgagacctct tgcgcgcgta gccccgcgct attgatcacg
     2881 ctcttggcca aaaccgagat cggttcgggc ccgccgatct cgagttcgat cccctgatcc
     2941 gtgacccgtc cggcgaccac ggggctctgg gttaccacct gagccccgtg cgcctcggct
     3001 tcgcgtcgga gcgccgccat caggccgtgg ctatcgatga tgcccgtgga cggcgagagc
     3061 aggccacgca cggccactac tttgggctcg agctcgtgca cctcgtccgc actcaacgga
     3121 accagatcgt gcacgccgtt ctcgcgggct tgggtgagca agcgatcgag caccgggatt
     3181 tcctgctccc gtgttgcgac gatcacctta ccgatccgga cgtgctcgac ctcgtgctcg
     3241 gcgcaataag cgtaaagcgc ggctttgccg gtcacacaga gggaggcctt gagagaaccg
     3301 gtcgggtagt agatcccggc gtggatcacc tccgagttgc gagaactcgt gtgggtgccc
     3361 atggccgatt cggcctctac caccagcacg tcgcgcgaag ccagcgcgag agtacgcgcg
     3421 atggctagtc cgaccacccc tgcgccgatc acgaccgcgt cgaccggctc ggccatcact
     3481 gaaccacgat cacgtcgccg ctctgcagcg ggaacatggc cgcgccggat tcattgttca
     3541 tgatcgcgtc ataggtgaag cggatgcgct ggaactgagg caccttgcgc aggaggaaga
     3601 tacggtcgtg atcaccgtac tcgctcagac cgcccgcctt ggccatggcc tgcagcatgg
     3661 atgcgggtgg ttcgagagtg agcgtgccgc cgctcttcac ttcgcccaga atagtgacgg
     3721 aaatcggcgt cgcttcctcg acgttgacgg tcacgcgggg ctcttgcaga tattgcttga
     3781 gccgcccttc gagctctttg gcgagcgcgg ccggtgtttt ccccgccgcc acgacctcgt
     3841 tcagcaaggg catcgccagg cgaccgtcgc gtcgcacgcg gcctttcagg ctcatgtctt
     3901 tctgctggta gacctcgatc gagatcgaat ctccaatgcc gatgatgtac tcgttcggcg
     3961 gggccaacgt cgagcgcgga acgcgcgtaa accaaacgta ctggccgtca ctcgcgcagc
     4021 cggtttcgaa agctgcgccc gcgacgagcg cgccgatcag caccgcgtga caggctcgtg
     4081 aacaggctcg cgtactggcc gcgctgcgac cgaaacgccc gccccgcacc acgctcggca
     4141 acggtttcga aagaaattgg aaaaacgact tcatgggcct gctgcctttc atacgctgac
     4201 cccgcgccgg aatccatggc tggaaaacgc gcacggtgac ccgagtgcct ggcatgccga
     4261 cacaagaagc tcttgctttg aaggaccacc tgactagatt cagggcacga tgaagcctcg
     4321 tgttggcttt gtagtcgaac aagcactagg tcacgtggct tacggccaga gcctgcgcaa
     4381 agccttggcg gggcgcgagg atctggagat cgtatggctg gaaattccct acggcttcga
     4441 gggcatgggt aaaatcccac tggtaggaag aaattggaca ctgcgtggca gtgcccgcgc
     4501 cttccgtgcg attcagcaag cccatgcgaa aaccccactc gatgcgttgt ttcttcacac
     4561 gcagaccatc agcgtctt
//
LOCUS       DASTKU010000949        31320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_186202, whole genome shotgun sequence.
ACCESSION   DASTKU010000949 DASTKU010000000
VERSION     DASTKU010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 31320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31320
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186202"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VFQ61_33635"
     CDS             complement(<1..1260)
                     /locus_tag="VFQ61_33635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959498.1"
                     /translation="MEEVASAASLSRSEARFENANTGGSLAWYKVRVSPRVWSAFALI
                     FCALFLAWHARRYLPFMADDAFISLRYSQRLIEGKGLTWDSSEHVEGYSNLTWVLACA
                     VLGALGTDWVLAARIVGAVSSFALLAAIAYAFRPGRGGGFWCATAAMLSVAVCTPIAV
                     WAQGGLEQPLVAALLAWSFALTLYRGDPARVSLRAPALLLALLTLSRPDGVFLAALFS
                     AGVGVSRGLHWAQLRRGAVLFTGAGIASLLQLAFRWFYYHDWVPNPARIKVSFTPERL
                     KEGLEYLNQALLFLSPAGIAILAAVTALSARAEARIRALPIGMLALCWAAYVAFIGGD
                     IFPGRRHVVVLIVAAAFALGELLFWMKRELSGRAPVFGIALAFVLVQATIQINDPEAK
                     AAQEEKWEWDGEVVGRLLKQAFARQSPL"
     gene            complement(1595..3532)
                     /locus_tag="VFQ61_33640"
     CDS             complement(1595..3532)
                     /locus_tag="VFQ61_33640"
                     /inference="COORDINATES: protein motif:HMM:NF012762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HET9959499.1"
                     /translation="MNGKYSRDSVSALRRDVTKVNRTGYFSLGVLTLIGAGLSFGCGA
                     SGKPETLETGSLESPPAADGAAQLAGGTDRRGIGLELAIAKANAAPDSSSLGKLTSAL
                     STPVNFPIDSRRSLFVSDEAIVGQITLKDVLSKLAGSVPDALTLYKNFWDLQNDDATG
                     VPGGTHCAPTLNTFPLTCPTMEGQQALDTDPFDDANLGLSNPQAYHAVALVNRLDLTD
                     PSGADCGEFRIIFGKNPQTGPQTFSRSLVIFEAVLPNPNPTLGLVGCRDIQTFWANLS
                     DPARSDADRGADLKKFFLDGAAGAAPVVSRGNYTGGRSGRIRTNQFFQDAEHISGLRP
                     WVLREFVLSASGAVTPRAVTTKANPFVGLFNDADSSNRFVDFLSRPDTVRALSVNDLN
                     GFNYPAGQVPDEFNAGQSQSSRFGTDTENTPNDYFGAFQSGGAQHRLRTRLQSTLTSL
                     RSNLTPENIVARAQSLSCAGCHIMSSEDPQPPLGLTNADGTEHPFPPVSTPFTHVDER
                     TTQNGRFLVSAAVEEFLLFRQQVLSNYFTRTEGFALSASVQVSSNWSGGYCVNVTLNN
                     RTTSAASVWKVRLSTPGAKLQTPWGSTATVQDSDVTFLPPSYAAPVNARGQLVFGFCA
                     TKNVSGTSPQISVVSATGFHP"
     gene            complement(3779..4648)
                     /locus_tag="VFQ61_33645"
     CDS             complement(3779..4648)
                     /locus_tag="VFQ61_33645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959500.1"
                     /translation="MLWRSLSYGALFCLTAERTAFAQRFPICIPGTPVCARAGVDGGS
                     QGTSQAQGAGGVQIGAGVQVQGSANPSGVRANGAANTAGGARADASAHAGAQAQGGPN
                     FGAWGNLDHGVTSYPPAGADSAYSAPRQRYSAPVRFGAGVTFCGTVKAGVFSGIKFGP
                     CFAVSFRTEVLTFELESQLLFGGVRDSIDWVFPMSFVVPLTQQRSLYQGLQLRFGGSP
                     IGVTFAKPKHGGNYLRFGLHAGLSYEYELGAAASWRVFDARAFLDFGTKREVDERGNF
                     LDSGGQLSTGLVF"
     gene            complement(4805..5362)
                     /locus_tag="VFQ61_33650"
     CDS             complement(4805..5362)
                     /locus_tag="VFQ61_33650"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET9959501.1"
                     /translation="MGFAKWVAAAMGTGALLTSVSALAEPATANSIQLGLGLRYGIEM
                     NEGDFNPWGLGLGLNGGITLPNAVYVGGVGEYFFGDKEEAGGVKLTGNIWQLMAEGGY
                     DVGLGPLFVLRPKVGLGLANSRSELCLTGLGCTKDNSSDFALAPGATFLVFLPMIKLS
                     ADVRYELVFAEETGKALIWTVGVGF"
     gene            5569..6993
                     /locus_tag="VFQ61_33655"
     CDS             5569..6993
                     /locus_tag="VFQ61_33655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9959502.1"
                     /translation="MLPFDSPPDGRLTLPRSVGRYELLNSLGSGGMGTVFRARQAQLD
                     KMVAVKILDARVASRPGGVTRFLREARAAAQIRHPHVIEVFDVGIDDGVAFIAMELLE
                     GVDLSSLLRDRKRLPLHKALDILMPVIAGVRAAHEAGVVHRDLKPSNLFLARRGRTLV
                     HPIILDFGVSRLLDEDERGVTPVTGSEAMIGTAAYVAPEQAMHGRLADDRSDQYSLGV
                     IVYECVTGVRPFEGQSVYQLLQSIATAEIRPPSQVNPSLPPTLDEVLLRALERDPEAR
                     YANVESFAAALLDFAAPRTWQLWGRELLGSAAGSNFDAVSATAVPSDHDPDDARAILA
                     PRPETLWAHARAGLLVGFVAGALVTGSAAWGWSRWRITDEAPLISRAVAGPGPSALTE
                     IRPLAAVSALRPAAAAEQAPLESPIVELGLKASAVPRSLPAKPALANPAPTKVVQTRP
                     RTEERRTAPTRGVEFGVNDAPILE"
     gene            7016..8101
                     /locus_tag="VFQ61_33660"
     CDS             7016..8101
                     /locus_tag="VFQ61_33660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959503.1"
                     /translation="MLVLSLVLQGSVGYSRTARAETGSPQQRGTEPLVRRGLELRRAH
                     RDSEALSAFKQAFAEEPTPMVRAQIALAEQALGQWLAADRDLSATLENPDAWVQAHRA
                     VLEKALGVIRSHLGLLEVVTNEPNLMLIVDDELVGTLPSAPKRLLPGRHVIRLRGPNG
                     TTREVALDLSAEERLTYHVEWPKSETAPVTNPTAAAPAPPVPTRALPPRADRKLRPQP
                     AERPSQSQARTIVGSVAAGIALAALVEGVAATLIRENYVKRYNSPECYPIRSEQCATQ
                     RDIARDLERVALLSYLVAGTAGASSLALFAWPLIGGSQPQGHSASGSIASGSMAPELV
                     AKEPGDPGHLKPANTLTGAQLVLSGQF"
     gene            complement(8126..9418)
                     /locus_tag="VFQ61_33665"
     CDS             complement(8126..9418)
                     /locus_tag="VFQ61_33665"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9959504.1"
                     /translation="MDQVAQVVIGRGSERASQRTNQAEGTRVHVSVPARTMSSMHARL
                     VNHRGHWSIQDLNSTNGTFVNGIRVQEAILGDRDVIEVGRVWFVFRQGLPTPSSCPVD
                     ADGSSVPGEMQVRTLVPHEAEQLRILNAIARTKLPVLLLGESGTGKELLARQIHAWSE
                     RSGALVPINSAALASSLLESQLFGYNRGAFSGAVRDEPGLVRAADRGTLFLDEIGDMP
                     AAAQVALLRVLQEGDVLAVGGTRPIPVDVRVIAATHRPLHSLAASGEFRADLLGRLRG
                     HLHTLPALRQRREDLGLLLAGLLSRVDPGSRIQRITADAARSLLSHDYPLNVRELEHA
                     LTRAAALAEDGTLHASHLDSAWVDLQGAAAPVVESERPPLSPEDELLRSSLLKQLSVH
                     RGNIADVARAMGKARMQIHRWLKRFEIDPASYRKGRAP"
     gene            9332..9550
                     /locus_tag="VFQ61_33670"
     CDS             9332..9550
                     /locus_tag="VFQ61_33670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959505.1"
                     /translation="MNPSSLGLVRPLRRTLGAAADNDLRDLIQAVPGAAEPRPFALED
                     EKQMSNQRLNLRGIVHLGLAGRLSVLGL"
     gene            9920..11653
                     /locus_tag="VFQ61_33675"
     CDS             9920..11653
                     /locus_tag="VFQ61_33675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959506.1"
                     /translation="MGRPQGVQRRAAWTALTCALGLGVLSLRCSKLAPDGQPAIQSGN
                     AGLTGGGASGTGGTAGRPSSSSDSSGGGIQAGEGGEPGGGAGHSSTEGSAPGGAAGAS
                     EGPSEAGGFNGSGGVDESGGFNGLGGSRGNSDAGSGQSASGGTSGTGNVTPPFTLLAE
                     RSLLESNPDTLKYITLERVLGRVIELPNVEATYAAMIQSFTPRPDETSPGPYCNDEPP
                     FNIEHGNATLNGFATPCPAEAGNLEYQLETWRPLAVVNRFDLAPADGANCGEQHLNFY
                     YDTTFVGQPASPVRAYLRFSAVIANPSPERGLEACRPVVDFWASLSGPAYDAPESRAR
                     ALEYALFGVTLGKSMQPTPEIAALIADGFQPFMSPQHFGTQGSVQWLYWGNEGLWYFF
                     EQAFVPEAEGYVRRQAMHSLPMDAMLNPHLKHEGCINFFLDAMSSLMTDDVAELRLEV
                     DRTCYAASSSTRDPSLLQGLTATELGKELKTRISSRLRTEYPDSPLYPEQIAARADFA
                     ATCVGCHAVPNSPWTRASSIAHISESFLEDCGTTRGEETRRCYSRSPVLKNVFLPRWL
                     DILSKFWTRER"
     gene            complement(11668..12321)
                     /locus_tag="VFQ61_33680"
     CDS             complement(11668..12321)
                     /locus_tag="VFQ61_33680"
                     /inference="COORDINATES: protein motif:HMM:NF015318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON domain-containing protein"
                     /protein_id="HET9959507.1"
                     /translation="MKSWLYARLSGVRLSGVRLLSAAVLVSVPLYACERPAGTFVGEA
                     QTMGGTSMGMLPPNTMLPPNTEEQAMGTQAFKSVSAGGMTFEWRIEGDRMHGRMSAPT
                     TGWVTVGFNERATLDGSKLVMGYVKDGAVVVEEHMADPPDHRPKVSLGGTNGIVEKRG
                     TESAGRTTIEFWMLLDTHSQPEVPIVPGREYHVVLAYSQEDDLYHHSMMRTAVQATL"
     gene            complement(12338..13729)
                     /locus_tag="VFQ61_33685"
     CDS             complement(12338..13729)
                     /locus_tag="VFQ61_33685"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HET9959508.1"
                     /translation="MQISSAAQSTLNSSPVLTDPEQLLRPFQRAFKPPEAFRVGTEAE
                     KFGVVREGLAPLPFEGEVSLSALFTRFTTRGWKPLREYSEGPVVGLQRGSSSITLEPG
                     GQLELSGAPFASIHDTAREFEQHFAELSEFSAELGILWLSAGFHPLAAPAEFPLVPKL
                     RYPVMRRYLSQRANRGLDMMFRTCTVQANLDYSDEQDALRKLRVALALQPIVTAMFAN
                     SPFVEGEISGRLSERGDVWLEMDPDRTGLLPFLWQRSATFRDYVDWALDVPCFLVRRG
                     AKVVENTGQTFRRFMSHGFGGTRAVESDWVTHLNTLFPEVRLKSTIEVRGADAQSKEL
                     TCALPALWKGLLYDERSLAKLEDLLSSLDPVSVADSRREMTRDGLNAQLGGRLVRHWA
                     EQVFEIAKQGLLRNPDRNEAGQCEDVYLAPLGQFLERGLTPAQLLVEQVRAGAPWRGS
                     GLREGIVEVTRIL"
     gene            complement(13955..14788)
                     /locus_tag="VFQ61_33690"
     CDS             complement(13955..14788)
                     /locus_tag="VFQ61_33690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET9959509.1"
                     /translation="MNNYDGGSSVRVAAIQMTSTEDVERNLQTALRLTERAARDGARL
                     IALPEAFAVLSPTRGKFEVAEELPAGGPILARFSECARKHECELVLGGFWERGRTPER
                     VRNSCVHLTSDGAVRAVYRKIHLFDVDLPDGTKLQESASVEPGSEVVVTDTIAGKLGL
                     SVCYDLRFPALFRQLVRRGATLLTVPSAFTLTTGKDHWHVLLRARAIESQCYVIAPAQ
                     TGCHFGSRVSYGHALICDPWGTVLAECGEGEGYALATVNHEVTQRIRRSLPNLDHAVL
                     D"
     gene            complement(14925..15473)
                     /locus_tag="VFQ61_33695"
     CDS             complement(14925..15473)
                     /locus_tag="VFQ61_33695"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9959510.1"
                     /translation="MNNVVPRAVRLESSFAYLVYRSARLLRYDFACLMAERGLEATQE
                     QWFILNKLAEQPGQSQTELCDGVFIDRPNLTRMLMTMERNGLLHRDSDRDDGRKLLVS
                     LTAKGIALHARIRDAVLARRERLFDGLTQKDLQQLRHVLSTLEANITSMRDAQNADGK
                     EPGRPSAPSRKGAASQSSDADE"
     gene            complement(15505..16335)
                     /locus_tag="VFQ61_33700"
     CDS             complement(15505..16335)
                     /locus_tag="VFQ61_33700"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9959511.1"
                     /translation="MNSPWYQRFQASSPSLPRLICIPYAGAGSGVFRPWAAHLSKVAE
                     VIAVQLPGRQERMDEPAFDSVSSLVPRLAEALLPLVDHPYVLFGHSLGAIIAFELALV
                     LEGAASLGPQHLIASARRSPSFPLETPALHRLPDAQLIGALRAMGGTPEAVLADPDLM
                     AALLPVVRADLKATEQHVSARGPRLQCPITVFGGLEDGLVSPEALTAWADATSGPSRV
                     VWFDGGHFFIHDYRDQVLESVSDILGVSTRGPRPDASVRSGAFVQNGVSHSGPKCVTP
                     "
     gene            complement(16349..24760)
                     /locus_tag="VFQ61_33705"
     CDS             complement(16349..24760)
                     /locus_tag="VFQ61_33705"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9959512.1"
                     /translation="METNALNSDVSQQELLTRSLVQIRRLKRELEELKSRNSEPIAVV
                     GMGCRFPGANSPEEFWRRLCAGYDASGEIPRSRWDAEAYYSSEPGRVGKMYTRRGSFL
                     ENIEDFDAGAFGISPREAAAMDPQQRLFLEVCWEALECSGQSLPRLQHGRTGVFVGIS
                     GREYGPLLLSREGLSSLDLHYLTGTLPSFVAGKLAFAFGFTGPALAVDTACSASLVAI
                     HLACNALRNGECDAALAGGTNLVLAPEWNIVLCRGTALAPDGRCKPFSARADGYARGE
                     GCGVVVLKRLSDALAEADPIAAVIRGSAINHDGRTSGMTVPSATAQREVIRAALRNAG
                     VPPNQVDYVEAHGTGTPLGDPIEAGALDVYGTGRAPDRPLLVGSVKSNIGHLESAAGI
                     AGLIKTILCLQHGHIPSHLHATELNPNLGLERIPAELTREAKSWPDTERRRVAAISSF
                     GASGTNAHLIVEQAPTATSQREPATRARPAHVFAVSAKNERALSGLLERWQAHLAENR
                     GLDLADLCFTAGESRAPFACRFSTAVADVQELADVLAKRREGELPAGSFERRVKGSAK
                     PRTAFLFTGQGSQYVRMGRELFEHQPSVRSILQQADEVLRPLLPLPLLDVMFSAETQS
                     DNAALLDDTLYTQPALFALEYAVARFWMDCGVEPSLLLGHSLGEYVAAAIAGVFGFEQ
                     GLRLIAARSRLMSEVAERGAMFSVVASRESLEPLLAGHEGELAIAAVNGPSQVVLSGA
                     REALEELIRELGAQGIKTKALAVSQAFHSPLMDSILPQFRRELEQVAWGAAKIPVVSN
                     VSGGVAGAEIATPEYWLSHTRRPVEFLECARSALEEGCSVFIEVGPKPTLASLVKPLA
                     QGDTTLIPSLRPGHSDARVMMTGLAQLFSLGTQLRWTGIDAGLSRRRAPLPSYPFQRQ
                     RFWPGPSAQVEAGAEPASELGASAGHVGTAAGAAGEKGAEREAGSAVLAAQRVASPLD
                     ILQYDLRLSPVKFPFLGDFLIANAKVVNIGVFVEVAFQTLAEALGGPAELLEFAVMKP
                     MIAAEQQQHLHVVLTPRGDQAFDLAIYGTEPEQSANQGEPERAATWSLHCTGKGRRAG
                     VAPERAPVDLQAFRQGAAATLAGSRFYAKMWRRGIQLGPAAQWLEEIWTNAEGDEAVA
                     RLRPPTPEDDRVSCELHPGVTDAAFQLVYAMIDDSLSQNYMVAGVRQFARPSRGGRPA
                     YCRARLARPPREQSKQAGIQAEITVYDERGTVLSVARGALLRPAAAHASDGQKAAAPR
                     SEPAPAAPSMGQSPVQSHSALAPAERLQWVRETLRKQLGAILRAAPSTIDEETPLLEL
                     GLDSLMLVELRAQLQTSIGLEPSLVQLLESPTLLELAQSLCEVEGSESASPSLDGPVS
                     PAPPRAEPRVAASHALSTIPRAAEGELSQVSPPAAFELETSDRIVPDRSRANEPFPLT
                     DMQQAYWLGRGSSFELGQTSTAFYLELDLRGIDLERLEVALNQLIHRHSMLRVTIQSD
                     GRNQVARHVPRYHIRVSDLRSESAEGEARELANTRALLRARVLPCEQWPLFEIRATLR
                     RDDWIRLHVCFDALAVDAWSTSILFREWAQLYADPFVPLEPLELNYRDYVLWLERQKS
                     SERYRAALGYWRERVRSLPPAPQLPVDSARLAQGRVPFAHHTFRLDAGRWERFKRAAR
                     EAGVTPSVAICTAYAEVLACFSASAHFTLNLLYFNRAPAHEQVKSVVGNFSTTVLLEI
                     DLREPRAFRENARRVRDGLARDLEHAAVSGVEVLRELNRSRGQGGQASMPVVFASTLR
                     GQEKQEMGEGLTRHLLSMAEEGREVHSSIRTPQVWLDHQVLEEDGELIVNWDVLEEAF
                     PAPLVETLFGVYRSLLVRLASDPKSFDAVVRFGMVPDSDLRPRREANATHAECLPECL
                     FEPFLRRVEAQPDAPAVIGSDQTLSYSELYARAASLAEQLIQRGVQPNEIVGVVSPKS
                     WRQVVAVLGVQLAGAAYVPVDPQLPQDRVDYMLRNSGARLVVTDAQSDAALNWSADVS
                     RVVVGEASPSRSGMALPPASRWLRRQKPGDIAYVIYTSGSTGNPKGVVIEHLGAFNTI
                     RDINTRFGLNETDRVFGISSLSFDLSVWDLFGALAAGAALVLPTPEELRDPQRWLHRI
                     KKDGVTVWNSVPALAQMLAEAAVASSEALDPLRLVMLSGDWIPTSLPGLLRSIAPELE
                     LVSLGGATEASIWSIYHQIRDVRPEWRSIPYGRPLQNQSFHVLDERLEPCPTYVPGDL
                     YIGGIGLAREYFGDPERTQRSFFHHPRTGDRLYRTGDLGRYTSDGEIEFLGRSDNQVK
                     IQGFRVELGEIESALAALPYVRAAAASAVGERSGPKTLVAYVVVQPELEVTSALLSEA
                     LARSLPAYMIPSRFVFLPELPLTPTGKVDRGSLARSISVEVATRRIVRPSNELESKLL
                     RIWQDILQREDLSVEDGFFDAGGQSFLAMRLMLRIKQEFRRELPLSTLFQHGTIRALA
                     MLLGGPSDEPTATNLIPMRAARGTSQPKAMFLVHPVGGNVLCYRDLAQLLPDEFEVHG
                     LQAHGLARGSSPLTSIEQMAEAYCRSITSRDDGRALRDHGVHLAGWSLGGVVAFEMAR
                     QLSFAGYAVSSLTLIDSVLPVPGREESDERRMLLGFFGDLSRLAGADTARVLDPVALG
                     RACDLEELMLQSLTLAKSSGLLPEHVGLEQIRPLWEVYRAGLQALAKYSPSSYPGQVT
                     HIWAQYGTQLEAPSQPHSSELGMAEPTPRTDWGAWVTGKFTDFGVAADHYSILRPPAL
                     EHTASLLARTLQQLGTSTSVTRVAGTLGGAQDE"
     gene            complement(24820..28185)
                     /locus_tag="VFQ61_33710"
     CDS             complement(24820..28185)
                     /locus_tag="VFQ61_33710"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HET9959513.1"
                     /translation="MSVQEMSKSNQDILRRATAAVKAARDQLESWKKSRSEPIAIVGL
                     GCRFPGSVYGAESYWNLLCSGTDTVRATPEDRFVGGSPVEADRSSLIRFGAFLDDVAG
                     FDAGFFGISPREARQLDPQQRIFLEVACEAIEDAGLPHEALRGSNTGVFLGVVNSDYL
                     HMQLLEPALADIYTINGGVHCMIANRLSYLLDLQGPSLAIDTACSGSLVAVHLACQSL
                     RSGESDLALAGGVNLILSAHATHAYVKAYPLAPDGRCRTFDARADGYVRGEGCGVVVL
                     KRLSDAIRDKDAIWALIRGSAVNQDGRTNGLTAPNGLAQERVIRRALQDAGLAPSRVS
                     CIEAHGTGTKLGDPIEVEALRNVYSAGDSSREQNARPCALGSVKTNLGHLEAAAGVAG
                     LIKLALCLKHREIPAHLHFQEANPEVPLAGSRFFVPGERMPWRSEGGTRVGAVSAFGA
                     GGTNAHVVLEEYDAPEPSIPTEEVSATYSLPLSGKTRDALLAQAASYREFLATHPSPL
                     PAVCASAAQRRTQHEQRAVVIARTKSEMGERLSRFISGVQDPCVVSGGVGVESRKVVF
                     VFSGQGSQWLGMGRALLEREPVFRQTIQDCEAALREFVSWSLVEQITSSDASALDDIT
                     VLQPALWAMQVALARLWQSWGASPDAVLGHSMGEVAAAVVANALSLQDGARIICKRSE
                     LARTIRGGGMLFVAESVDKLEAALGEFRDSVAVAAENGPVSTVLSGAASSIDALHARL
                     TASNVFCRRIRVDFASHSPQTAPLMAPLRAELESVSPRAGSIPIQSTLRGQLLTGSEM
                     DANYWAEHLRQRVRFLEGMRALLNAGHEVFIEVSPHPILTTPMIEAAEFEGKTVAALA
                     TLHRDRQVEESLALARAQLHVLGLSSRALGERQTPVAHERLPRYVWQHRPYWFKERPA
                     AGQSIPSLDRLPVATAPQDAVNPSRANQTNVSQGGAGAVGSLRLRWQSARADRRAALL
                     EDSVLSEVASALGADPENVERDAGFFQMGMDSIVAVRLHLRISELLGLTLATRVVFEY
                     PSVSKLSHYLHARLSNDSRPGDLERSAGESVGDNRGKSSGLKVPDSPTVALSSPAAPS
                     ASSGRKATLTLDEGDLLRSLAMELAELETNATV"
     gene            complement(28163..>31320)
                     /locus_tag="VFQ61_33715"
     CDS             complement(28163..>31320)
                     /locus_tag="VFQ61_33715"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HET9959514.1"
                     /translation="LIAAGYSHFIELSPRPVLARELKQALKGSGHVLVPLPKELEESR
                     GLLRTIEQIHGLESDESRLARARSASVRRFCFPLSAHSEPALVQRAKELAQHLRKATR
                     DVASDLAYTLSSRRDHQQQRVSFVARNTGELADQLEAFEFGSPVRGLARGRAELTNGQ
                     VAFVFPGQGGQWLGMGRELTQVEPEFRRALQRVARVAHERHGRRLLDELSQASEEDLK
                     RSRFVQPLIFAFQVAFAELWRSFGVEPAVVVGHSMGEVAAAHVSGVLSLEDAVDVICI
                     RSRLLDRVSGRGAMLLTELEPAEAEALVAGRPAVAVAALNSPRSCVLSGAESALREVE
                     RELLAKTMFCRWVRVDVASHSPQVDELLVDLEHSLAHLRPRSAKVPFLSTVRGELLSG
                     LELDAHYWVRNLRDPVRFAPVVGALLRQGHRRFLEISPHPVLLPALQQCAAGEHGGAG
                     EHGAVLVTGCTRRDEPEHATLLATLGALHAHGASVRWDWVHRQGGQVELLPTLPFQRE
                     RYWIESEAQSPTARIERALEPSRSSPMQLPAMAHGAAESLLSTGAQAISGPHAATGAR
                     LSVPRLWLFHIDPARAPELAQHQLGEIPLLSASFWLEWALYGAGSILPEGSIDFQIEF
                     KEALRLEGSGALEAQMMARELSPGKVGVWLHTRRLETDEWCEHVVFTGQAGPPGLLPA
                     RSEIDSSTDSGAWAQEECRLEGDALYKRLAQRGLHCGERLRVLTRLQLSPEGALGRVG
                     VESASNNIRVPLALEAAWQALALCLPQRLDQYLVPSRIVRGRLWSGSDSARSNCAQLN
                     SAAIVQARVLSGSTPANHTDTLRAAAPPTNVHAALAMLDDAGRPCLSLDHIELVPVPV
                     SMFQARGGAGAGTASVHDARTDSVGTRTTTSAGASGAPNATPNFRELLRQAPSEAAQK
                     ALLLGHVRAALSIVLALEGSRIGSDTPFRDLGMTSLMGLELRNRLEGSLGLRFSAATI
                     WNYPTLSRFCGYLAGQLDLRNRESAPQGESSARAPETTSTAAPTERDSEVESDLAREL
                     AALEQLLDSQSGNSHVSSGNV"
BASE COUNT         5967 a        10309 c         9508 g         5536 t
ORIGIN      
        1 cagcggcgat tgacgggcaa aggcttgctt caggagccgg cccacgactt ccccatccca
       61 ttcccacttc tcctcctgcg ccgccttggc ttctggatca ttgatttgaa tggtggcctg
      121 cacgagcacg aaagcgagcg cgattccgaa caccggcgcg cgcccggaga gttcacgctt
      181 catccaaaat aggagttcgc cgagcgcaaa tgcggccgcc acgatcagca ccacgacgtg
      241 ccggcggcca gggaagatgt cgcctccgat gaatgcgaca tacgccgccc agcacagggc
      301 caacatgcca attgggagag cgcgaatccg cgcctccgca cgtgcagaca gggccgtgac
      361 cgcagccagt attgcaatcc cggccggcga taggaacaac aacgcttgat tgaggtactc
      421 cagcccctct ttcagtcgct ccggcgtaaa ggacaccttg attcgcgcgg gattcggaac
      481 ccaatcatgg taatagaacc atcgaaaggc gagctggaga agcgaagcga tccccgcgcc
      541 cgtgaacaac acggcgccac gccggagctg cgcccagtgc aggccgcgcg agacgccaac
      601 tccggcgctg aacagcgccg ccaaaaacac cccatccggt cgcgacaagg tcaatagtgc
      661 gagcaacaac gcaggagcgc gcaggctcac gcgggcaggg tcgccccgat agagtgtcag
      721 cgcgaacgac cacgccagca gcgccgccac cagcggctgc tcgagccctc cctgagccca
      781 aaccgcgatc ggggtgcaca ccgcaacact cagcatcgcc gcggtcgcac accagaaacc
      841 gccgccgcga ccaggccgaa acgcgtaggc tatcgccgcc aacaaggcaa agctcgagac
      901 cgcacccacg atgcgcgcgg ccagcaccca gtccgtgccg agagcgccaa gcacggcgca
      961 cgccaagacc caggtcaggt tcgagtagcc ctcgacgtgt tcactcgaat cccacgtgag
     1021 ccccttgcct tcgatcaaac gctgcgaata ccgaagcgag atgaaggcgt catcggccat
     1081 gaagggtagg taacggcgcg catgccaagc cagaaacaac gcgcaaaaga tcagcgcgaa
     1141 ggcggaccac acccgcgggg atacgcgaac cttgtaccaa gccagcgagc ccccggtatt
     1201 cgcgttttcg aagcgcgcct cggaacgcga cagggatgcg gctgaggcca cttcttccat
     1261 caagagaggc tctccagtag gccgtgaact aggtctgcgc gcggctcttc cgttctacac
     1321 tcttccccgc tctcttctcc ttcttccgac ttctctccgg tcgaagcaag ccccacgtgc
     1381 cgctgattgt gccagtgtgt gtcacgagaa cgaagccacg taccctgatc ggtggagcaa
     1441 aacgcgcgca agtagaccac gggcgagtgc tagcaatcgc cgtacctact gagcgaggct
     1501 cgacggacca gcccgccgcc gagagcagcc gcgaagctgc cgtcgcaagc ttcgcggctt
     1561 tcctcacgtg cacatgaatc ggacgggacc gggactacgg atgaaaccca gtcgccgaaa
     1621 cgacgctgat ctgcggtgac gttccggaca cattcttggt cgcgcagaac ccgaatacca
     1681 actgccctcg agcgttgacg ggcgccgcgt agctcggcgg caggaacgtg acgtcactat
     1741 cctgaacggt agccgtggat ccccacgggg tctgcagctt cgcgccgggg gtcgagaggc
     1801 gcactttcca aacgctcgcg gcagacgtgg tccggttgtt caaggtcacg ttgacgcagt
     1861 agcctccgct ccagttggag ctgacttgaa cgctcgcaga gagcgcgaag ccttcggtcc
     1921 gcgtgaagta gttggagaga acttgctggc gaaacagcag aaactcttcc acggccgcag
     1981 acaccaagaa cctgccattc tgcgtggtgc gttcgtccac gtgagtgaac ggagtactta
     2041 ccggcgggaa cgggtgctcc gtgccatccg cattcgtcag gccgagcggt ggctgcggat
     2101 cttccgaaga catgatgtga caaccggcac acgagagcga ctgcgcccgc gcgacgatgt
     2161 tctcgggggt gagattcgag cgcagagacg tgagcgtgct ttggagccgc gtacgcagac
     2221 ggtgctgcgc cccgccggac tggaaagcac cgaagtaatc gttcggggtg ttctcggtat
     2281 ctgtcccgaa gcgagaggac tggctttggc cggcgttgaa ctcgtccggg acttgtcccg
     2341 ccggataatt gaaaccattc aagtcgttga cggagagcgc tcgaaccgtg tccggccggc
     2401 tcaagaagtc cacgaagcga ttggagctat ccgcgtcgtt gaacaggccc acgaacgggt
     2461 tggccttggt ggtcacggcg cgaggcgtaa cggcgccgga cgcgctcaac acgaactcac
     2521 gtagcaccca cggccgcagc cctgaaatgt gctctgcgtc ttggaagaac tggttcgtgc
     2581 gaatgcgccc ggaacgccca cccgtgtagt taccgcgtga aacgaccgga gccgcgccgg
     2641 ccgcgccatc caaaaagaac ttcttcaggt cggcgccacg atcggcgtcg ctgcgcgccg
     2701 ggtcgctcag gttggcccag aaggtttgaa tatcccgaca accgaccaag cctagagtgg
     2761 gattcggatt gggcaatacg gcttcgaaga tcacgagcga tcgagagaac gtctgcggcc
     2821 ccgtctgagg gttctttccg aagataatcc ggaactcacc acagtctgcc cccgacggat
     2881 cggtgagatc cagccggttg acgagcgcga cggcgtgata cgcttgcggg ttcgagaggc
     2941 ccaagttcgc atcgtcgaac ggatcggtgt cgagggcctg ttgcccttcc atggtcgggc
     3001 aagtaagcgg gaaagtattg agggtcggcg cgcagtgggt tccacctggc acccccgtgg
     3061 cgtcatcgtt ctgaagatcc caaaaattct tgtagagagt cagcgcatcg gggaccgaac
     3121 cagctagctt gctcaacacg tctttgagcg tgatttgccc gacgatggct tcatcactca
     3181 cgaagagtga acgccgactg tcaatcggga agttcacggg agtggacaaa gccgaggtta
     3241 gtttacctag cgagctcgag tctggcgcgg cgttggcctt cgcgatcgcg agttcgagac
     3301 caatgcctcg tcggtccgtg ccgcccgcga gctgagccgc cccgtccgcg gcgggcgggc
     3361 tctccaagct gccagtctcg agagtttccg gtttgccaga ggctccgcaa ccgaagctca
     3421 acccagcgcc aataagagtc agcacgccga gactaaagta acccgtgcgg tttactttag
     3481 tgacgtcgcg tcgaagtgcc gagaccgagt ctcgggagta tttcccattc atcttgtgcc
     3541 gtccttcaag tggtgcgcgc tcgatcgcgc gagcgcgccg cccgccacgt gcaacgcgcg
     3601 tgcccctaaa tatccaagtg caccgcattc tggatccggg ctccttctcc ctcagaacgg
     3661 ctcgatttcc gatctccaac cctcgagcgt agctccttgc ctcgcgccaa gcgccgcagg
     3721 agaccgtcgt cagtaacacc ccgtcacagc attgtggtgt tgcgctgggt tacagccgtc
     3781 agaaaacgag tccggtgctc agctggccac cggagtccaa gaagttgccc cgctcgtcga
     3841 cttctcgctt ggtaccaaaa tctagaaagg cccgcgcgtc gaacactcgc caggacgccg
     3901 ccgctccgag ttcgtactcg taagaaaggc cagcgtgcag gccaaagcgc aggtaattgc
     3961 cgccatgctt cggtttggcg aaggtgactc cgatgggtga tcctccaaag cggagctgca
     4021 atccttggta gaggctacgc tgctgagtca aaggaacgac gaaagacatg gggaacaccc
     4081 aatcaatcga atcgcgtact ccgccgaaca gcagttgact ctccagctcg aaggtcagca
     4141 cctcggtccg aaacgacacc gcgaagcagg ggccgaactt gatccctgag aacactccgg
     4201 cttttacggt tccgcaaaag gtcacgcccg cgccgaaacg tacgggggcc gagtagcgtt
     4261 gccggggcgc tgaatacgcc gaatcggctc cggccggagg atatgaggtc acgccgtgat
     4321 cgagattgcc ccacgcgcca aagttcgggc cgccctgggc ctgtgctccc gcgtgggcgc
     4381 tcgcgtcggc ccgagcacca cccgccgtgt ttgcggctcc gttcgcccgc acacccgaag
     4441 gattggcgga accttgtact tggacaccag cgccgatctg aacgcctccc gccccctgtg
     4501 cctgactcgt cccctgtgaa ccgccgtcca cccccgctcg cgcgcagacg ggtgtaccgg
     4561 gaatacagat tgggaatcgc tgagcgaagg ccgtgcgctc ggcggtcaag cagaagaggg
     4621 ccccatatga aagagatcgc caaagcatga gaatagaagt cccgagcgag accaaccgat
     4681 ggttcctacg ctcgggacgg cgcgaatctc tccactcgaa agtggctact ctgcggatat
     4741 cgggcggggc gactgactcg acgcagccct caggtcgtga agagtgcgag cgcccgcgcg
     4801 ggcgtcagaa cccgactcct accgtccaaa tcaaggcctt gcccgtctcc tccgcgaaca
     4861 ccaactcgta tcgcacgtcc gccgacagct tgatcattgg gagaaatacg agaaaggtcg
     4921 cgcctggtgc cagagcgaag tcgctcgagt tgtccttcgt gcagcccaac cccgtcaagc
     4981 agagctctga tcggctgttc gccaaaccca agccgacctt ggggcgcagc acgaagagcg
     5041 gcccgagccc gacgtcgtag ccaccctccg ccatcaactg ccagatgttg ccggtgagct
     5101 tgactccgcc ggcctcctcc ttgtcgccga agaagtactc cccgacaccg cccacgtata
     5161 ccgcgttggg cagcgtgatg ccgccgttca agcccaggcc caagccccaa ggattgaagt
     5221 caccctcgtt catttcaatg ccgtaacgga gccctaaacc cagctggatg gagttcgccg
     5281 tggcgggctc ggccaaggcc gataccgagg tgagcaaagc gcccgtgccc atcgccgccg
     5341 caacccattt tgcaaaaccc atgaagcctc ctgataagtc gtctggagta agtcgatccc
     5401 ccgaggaaat ttccccgaga ttccgggcag ctttccatgg gcgcaccggt tctgtaaaga
     5461 ccgcggccga tgcgccggac tgacccgagt caatccacgc cgaagcggca atgaagtttg
     5521 gcctgcaggc tggctggact tcaccacccg tggtgtatgt ttgcgcgcat gctccccttc
     5581 gactcccctc ccgacggacg gctcaccctg ccgcgaagcg tgggtcgtta tgagctcttg
     5641 aactcattgg gaagcggcgg aatgggtacc gtgttccgag cccgtcaggc ccagctcgac
     5701 aagatggtgg cggtcaagat cctggacgcg cgggtcgcga gtcgccctgg tggggtgacg
     5761 aggtttctgc gcgaagcgcg ggccgcagcg cagattcgcc atccccacgt catcgaagtg
     5821 ttcgacgtgg gcatcgacga cggcgtcgcc ttcattgcca tggagcttct ggagggcgtc
     5881 gacttgtcga gtctgttgcg cgatcgcaag cgactacctt tacacaaggc tctcgatatc
     5941 ctgatgccgg tgatcgctgg cgtgcgtgcc gcccacgaag cgggcgtcgt tcatcgagat
     6001 cttaagccaa gcaatttgtt cttggcgcga cggggtagaa ctctcgtgca cccgatcatc
     6061 ctcgatttcg gtgtgtctcg tctgctcgac gaagacgagc gcggggtgac gcccgtcacg
     6121 ggctcggagg ccatgattgg taccgcagca tacgtggcgc ccgagcaagc catgcacggt
     6181 cggctcgcgg acgaccgaag tgaccagtat tccttgggtg tgattgtcta cgagtgcgtc
     6241 acgggcgtgc gcccgttcga gggccagagc gtctatcaac tcctgcagtc catcgcgacg
     6301 gcagaaatac gccctccgag ccaggtgaat ccgtcgctac caccgacact ggacgaggtg
     6361 ctcttgcggg cgctcgagcg cgatcccgaa gcccgctacg ccaacgtcga gagctttgcg
     6421 gcggctttgc tggactttgc ggcgccccgc acttggcaac tgtggggacg agaattgcta
     6481 ggatcagccg ccggctcgaa cttcgacgcg gtctccgcaa cggcagttcc gtcggatcat
     6541 gaccccgacg acgcgcgcgc aatactggct cctaggcccg aaacgctctg ggcccacgcg
     6601 cgagctggtc tcttggtagg cttcgttgcg ggagctctcg tgacgggaag cgcggcctgg
     6661 ggttggtcac gttggcgcat aaccgacgag gcgccgctga tttcgcgcgc tgtcgccggt
     6721 ccggggccga gcgcgcttac cgagattcga cctctcgctg cagtatccgc gctgagaccc
     6781 gccgcggcgg ccgagcaagc tccgcttgag tcgccaatcg tggagctggg tctgaaggcg
     6841 tcggcggtgc cgagaagttt gccggcaaaa ccagcgctcg cgaacccggc gcccaccaaa
     6901 gtggtgcaga ctcgcccccg gaccgaggag cgtcgaaccg cgcccaccag aggagtcgag
     6961 ttcggagtca atgatgcgcc cattctcgaa tagcgcgcgc tcgagacgcg cttggatgct
     7021 cgtcctgagt ctcgtcttgc agggttccgt cggatattct cgcaccgcgc gtgccgaaac
     7081 cggcagtccc cagcaacgcg gaacggagcc gctcgtgcgc cggggtctgg agctgcgccg
     7141 cgcgcatcga gactcggaag ctttgagtgc tttcaagcag gcgtttgccg aggagcccac
     7201 gcccatggtg cgcgcgcaaa ttgcgcttgc cgagcaggca ctcggccaat ggctagcggc
     7261 agatcgggat ttgtcggcaa cgctagagaa ccccgatgcc tgggtccagg cgcaccgcgc
     7321 ggtgttggag aaggcattgg gcgtgatccg ctcccacctc ggtctgctcg aggtggtcac
     7381 gaacgagccc aacctaatgc tcatcgtgga tgacgagttg gtcggcactt tgccctccgc
     7441 ccccaagcgc ttgttgcccg gccgacatgt cattcgtctg cgcggtccaa acggcacgac
     7501 tcgagaggtc gcgctggacc tgtccgctga ggagcggctc acctatcacg tcgaatggcc
     7561 gaaatccgaa acggcgccgg tcacgaaccc aaccgctgcc gcaccggcac cacctgttcc
     7621 gacacgagct ttgcctcccc gagctgatcg gaagctgcgc ccgcagcccg cggagaggcc
     7681 ttcccaatcc caagcacgaa ccatcgtcgg ctctgtcgcc gcggggatag cgctcgcggc
     7741 gctggtcgaa ggcgtggccg cgaccctgat tcgggaaaac tacgtgaagc gctacaacag
     7801 cccggaatgc tatccgatcc gtagcgagca atgcgcaacc cagcgggata ttgcacgtga
     7861 cttggagcgc gtggcgctcc tcagttatct cgtggccggg acggccggtg cgagttcgct
     7921 cgctctcttc gcctggccgt tgatcggcgg ttcacagcct caagggcaca gcgcgtctgg
     7981 gtctattgcg tctgggtcta tggcgcctga gctcgtggcc aaggagcccg gtgacccagg
     8041 gcacttgaag cccgcgaaca cactgacggg agcccagctc gtgctttccg gacaattttg
     8101 aagctcactg acgtgggttc gttggttagg gcgctctccc tttgcggtac gatgcggggt
     8161 cgatctcgaa gcgcttcaac cagcgatgaa tctgcatcct cgctttgccc atcgcacgcg
     8221 cgacatcggc gatatttccg cggtgcacgc taagctgctt caatagggat gagcgcagta
     8281 gctcgtcttc gggagagagc ggcgggcgct cggattccac cacaggcgcc gcggcgcctt
     8341 gcaagtccac ccatgccgag tccaagtgcg aggcgtggag cgtgccatcc tctgcgagcg
     8401 cggccgctcg cgtcaaagcg tgctcgagct cccgcacgtt gagcgggtaa tcgtgagaca
     8461 gcaggctgcg cgcggcatct gccgtgatgc gctggatgcg acttccggga tcgactcgac
     8521 ttagcaagcc ggcaagcagc aagcccaagt cctcgcgccg ctgccgcagt gccggaagcg
     8581 tgtgcaggtg cccgcgtaag cgacccaaca agtcggctcg aaactcaccc gatgccgcga
     8641 gcgagtgcaa tggccggtgg gtcgcggcga tcacgcgcac gtccactggg attggacgcg
     8701 tcccgcccac cgcaagtacg tccccttctt gcagcacgcg cagcaacgcc acctgtgccg
     8761 ctgcgggcat gtccccaatt tcatctagaa aaagcgtgcc gcgatccgcc gcgcgcacca
     8821 aaccgggttc atctcgcact gctcccgaaa aggcccctcg gttatacccg aacagctgag
     8881 actcgagcag actgctcgcg agcgccgccg agttgatggg cacgagcgct ccgctccgct
     8941 cactccaagc atgaatttgg cgtgccagca gctccttgcc cgttcccgac tccccgagta
     9001 gcagcactgg cagtttcgta cgcgcaattg cattcaaaat gcgaagctgc tccgcttcat
     9061 gcggaaccag ggtgcgtacc tgcatttccc ccggcacgct cgagccatcg gcatccaccg
     9121 gacaactcga aggagtggga agaccctggc gaaacacgaa ccagacgcgc cctacttcga
     9181 tcacgtctcg gtcacccaga attgcctctt gcactcggat gccgttgacg aatgtgccgt
     9241 tggtcgagtt caggtcctgg attgaccagt gccctcgatg attgacgagt cgagcgtgca
     9301 tactcgacat cgtgcgggca ggcaccgaga cgtgaacccg agttccctcg gcttggttcg
     9361 tccgttgcga cgcacgctcg gagccgcggc cgataacgac ttgcgcgact tgatccaagc
     9421 cgtaccgggc gccgccgagc cgcggccgtt cgccctcgaa gacgagaaac agatgagcaa
     9481 ccaacggttg aacttgcgag gcatcgtcca cctcggactc gctggccgtt tgagtgtgct
     9541 ggggctctga gggcgttctc accaaccggc cactttaagt cagcgccgcg cactttgcac
     9601 gcgatggcgt gcggtggtgc ctcgcgccgc ctcgttttac gcaggccatg ttttacgcag
     9661 gccatgtgga gaccgtgctc gtgatgccgg acgcttcgac gttgctgtac tggtttctcg
     9721 gtatactggt cgtcaggtgc ggttcgggtt tgctcaagtg gtttgacgta gttcatcttg
     9781 aggccatcct gggcgcatgg ttgcctccgc ttcgcgcgcc gcttcctcca gcgcatcatc
     9841 ctcgcgagtt accgcttcga cggtagcctc tcccgtagca tcgtcgcgcg cttcattccg
     9901 cgctgctatt tctgccggag tggggaggcc acaaggcgtg cagcgccgag ccgcttggac
     9961 agcgctcacg tgcgctctcg gcctgggagt tctcagcctg cgctgcagca agctcgcacc
    10021 cgatgggcaa ccggcgatcc agagtgggaa tgccgggctc accggcggcg gcgcgtccgg
    10081 cacaggaggc acggcaggcc gtccgagttc gagtagtgat tctagcggag gaggcatcca
    10141 agccggtgaa ggcggtgagc ctggcggtgg cgcggggcac tcttcgactg aaggttcagc
    10201 accgggcggc gccgcgggag ccagtgaagg gccgtcagag gcaggtggat tcaacggatc
    10261 aggtggcgtc gacgaatccg gtggattcaa cggattaggt ggctcgcgag ggaacagcga
    10321 cgccggcagc gggcaaagcg cgtcgggggg cacgagcggc acggggaatg taactcctcc
    10381 gtttaccttg ctggccgaac gttcattgct cgagagcaac cccgatacac tgaagtacat
    10441 caccctcgag cgggtgctgg gccgcgtcat agaactgccc aatgtcgaag ccacgtacgc
    10501 cgctatgatt cagagtttca ccccgcgccc ggacgagacg agcccgggac catattgcaa
    10561 tgacgagccg ccgttcaaca tcgaacacgg gaacgcgacg ctcaacggat tcgccacccc
    10621 ctgccccgcg gaggcgggca acctcgagta tcagctggag acctggagac cgctcgccgt
    10681 cgtcaatcgc ttcgatctgg cgccggcaga cggagccaat tgcggcgagc agcatctcaa
    10741 cttctattac gacacgacgt tcgttggcca gcccgcgtcg ccggtacgcg cctacttgcg
    10801 cttttcagca gtgatcgcca acccctcgcc cgagcgcgga ttggaagcct gtcgtcccgt
    10861 ggtggacttc tgggcaagcc tgagcggccc cgcgtacgat gcgccggagt cacgggctcg
    10921 cgccctcgaa tacgctctat tcggcgttac tctgggcaag agtatgcaac cgacgccgga
    10981 gatcgcagcg ctgattgccg acggatttca gcccttcatg agccctcagc actttggcac
    11041 tcaagggagt gtgcagtggc tatactgggg caacgaaggg ctatggtact tcttcgaaca
    11101 agccttcgtg cccgaggcag agggctacgt gcggcggcaa gcgatgcata gcttgccgat
    11161 ggacgccatg ctgaatccgc acctgaaaca tgaaggctgc atcaacttct tcttggatgc
    11221 gatgtcatcc ctcatgaccg acgacgttgc ggagctccgg ctggaagtgg accgaacttg
    11281 ctatgcggca agctcttcca cgcgagatcc ttccctactt cagggcctga ccgcgaccga
    11341 actgggtaag gaattgaaaa cacgtatctc gagccggctc cgcaccgagt atcccgattc
    11401 ccccttgtac cccgagcaaa tcgcagccag ggccgacttt gccgcgacct gcgttggctg
    11461 tcacgcggtc ccgaattcac cttggaccag agccagcagc atcgctcaca tcagcgaaag
    11521 tttcctcgag gactgcggca cgacccgcgg ggaagagact cggcgctgct actcgcgctc
    11581 accggtgctg aagaatgtat tcctgccccg ttggctcgat attctctcga agttttggac
    11641 tcgcgaacgt tagagccaag tcggcaatca tagcgtcgct tgcactgcag ttcgcatcat
    11701 cgaatgatga tataggtcgt cttcctggct gtaggccagt actacgtgat attcccgacc
    11761 gggaacgatc ggcacttcgg gctgactgtg tgtatcgagc aacatccaga actcgatggt
    11821 agtccgtccg gcgctctccg tcccacgttt ctcgacgatg ccattagtcc cacccaggga
    11881 gactttgggg cgatggtcgg gcggatccgc catgtgctcc tcgacaacga ccgcgccgtc
    11941 tttgacgtat cccatcacga gcttgctgcc atccaaggtg gcgcgttcgt tgaatccgac
    12001 ggtgacccag ccggtggtgg gcgcgctcat gcgcccatgc attcgatctc cttcgattcg
    12061 ccattcgaac gtcatgccgc ccgctgaaac tgacttgaat gcttgtgtgc ccattgcttg
    12121 ctcttcagtg tttgggggga gcatggtgtt tggagggagc atgcccatac tggttccgcc
    12181 catggtttga gcctcgccca cgaaagtgcc tgccggccgc tcgcaggcgt acaaaggtac
    12241 gctcacgagt accgcagcgc tcaacagcct cacgcccgac agcctcacgc ccgacagcct
    12301 cgcgtagagc cagctcttca ccgtcgggcc gactctatca gagaattcgg gtaacctcga
    12361 caattccctc tcgcaatccc gaacctcgcc acggcgctcc cgccctgact tgttcgacta
    12421 acagttgagc gggcgtaagc ccacgttcca aaaactgccc gagcggggcc aagtagacat
    12481 cctcgcactg cccggcctcg ttccggtctg ggtttcgcaa caatccctgc ttcgcgattt
    12541 cgaagacctg ctccgcccaa tgtcgcacca ggcgacctcc gagctgcgcg ttcaggccat
    12601 cccgagtcat ctcgcgacgg ctgtcggcaa ccgacacggg gtcgagagag ctcaacaggt
    12661 cttctagctt ggcaagcgat ctctcgtcgt acagcaagcc tttccacaat gcgggcagtg
    12721 cacacgtgag ttctttcgac tgcgcgtcag cgccacgcac ttcaatcgtc gatttgagcc
    12781 gcacttccgg aaagagcgtg ttcaagtgcg taacccaatc cgactccacg gcacgagttc
    12841 cgccgaaccc gtggctcatg aagcggcgga aggtttgacc agtattctcg actaccttcg
    12901 ctccgcgccg cacgaggaag cacggcacat cgagcgccca atcgacatag tctcgaaagg
    12961 tcgcgctgcg ctgccacaaa aacggcaaga gcccggttcg atccggatcc atctcgagcc
    13021 acacatcacc ccgctccgac aagcgccccg agatctcgcc ctccacgaac gggctgttgg
    13081 cgaacatggc ggtcacgatt ggctggagcg cgagcgcgac acggagcttt cgcagggcgt
    13141 cctgctcatc cgaatagtcg agattggcct gcaccgtgca ggttctgaac atcatatcta
    13201 gcccgcggtt cgcacgctga ctcaagtatc gtcgcatgac cggatagcga agcttcggca
    13261 ccagaggaaa ctcagcggga gcggcgagcg ggtgaaagcc cgcactcaac cacaatatcc
    13321 ccagctcagc cgaaaactca ctcagttccg cgaagtgctg ttcgaactcc cgcgcagtat
    13381 cgtgaatcga tgcgaagggt gcccccgaga gctcgagctg cccgccgggc tccagcgtga
    13441 tcgaactcga gccgcgctgc agcccaacta cgggcccctc ggagtattcg cggagtggct
    13501 tccacccgcg cgtggtgaag cgtgtgaaga gcgcgctcag actcacctca ccttcgaacg
    13561 gtagcggcgc gagcccttct cgtactaccc cgaatttctc ggcttccgtg ccaacccgaa
    13621 aggcctcggg aggcttgaac gcgcgctgga acggtcgaag cagttgctcc ggatccgtca
    13681 acacggggct cgagttgaga gtcgactgtg ctgctgaact gatttgcatt tcattcattt
    13741 tgcacttcat tcattttgca cttcactcat gatgcatccc taacccaagt tcggaatctg
    13801 gcttgccgcg acacctccgc tcgagctcgg cggcgaaaag ccatgacggg cctattgccg
    13861 agttcagacc ccggaatatg tcgtcgtatc tcacgtgcgc cgcactggag cccctcgcgg
    13921 gctcacactg atcgagcgct attcgttcgt agagtcaatc caacacggcg tgatcgaggt
    13981 ttggtagaga gcgcctaatc cgctgcgtca cctcgtgatt gacggtcgcg agcgcgtaac
    14041 cttcgccttc gccgcactcg gcaagaacgg taccccaagg atcacaaatc aacgcgtgac
    14101 cgtaggagac ccgagaaccg aaatgacatc cggtctgcgc aggcgcgatc acataacact
    14161 gcgattcgat ggcgcgggcc cggagcaaga cgtgccagtg gtccttaccc gtggtcaaag
    14221 tgaacgcgga aggcacggtc agaagcgttg ccccacgccg gaccagctgc cggaacagcg
    14281 cgggaaagcg cagatcgtag cagaccgaca gccccagctt gcccgcgatc gtatccgtca
    14341 ccacgacctc actcccgggc tccacactgg cagactcctg cagtttggtg ccatccggta
    14401 agtcgacgtc gaagaggtga atcttgcgat atacggcgcg cactgcgccg tctgacgtga
    14461 gatgtacgca ggagtttcgt actcgctcgg gggtgcggcc ccgctcccag aatccaccta
    14521 ggacgagctc gcactcgtgc tttcgcgcac actcggagaa gcgagccaga atcgggccgc
    14581 ccgcagggag ctcttccgcc acttcgaatt ttccgcgggt ggggctcaac accgcgaatg
    14641 cctcgggcag cgcaatgagg cgagcgccgt cccgcgccgc tcgttcggtc agacgcaggg
    14701 cagtttgcaa gtttcgttcg acgtcttcag tgctcgtcat ctgtatggcg gcaacgcgaa
    14761 cactcgatcc cccatcgtag ttattcatgg catcacgtct acttcgtact ggcttggttt
    14821 cctgcgctcg tgcttttttc gagttcgctt cgtcaccggc gcccggtgtc aaagcgcgct
    14881 tgattcctgc tccggaattc ctgcgatccc attgcctccg caagctactc gtcggcgtcg
    14941 ctgctctgcg acgcggcgcc ctttcgcgag ggagcgctgg gccgccctgg ttctttgcca
    15001 tcggcgttct gagcgtcgcg catcgaggtg atgttggctt ccagggtgct cagaacgtgc
    15061 ctgagctgct gcagatcctt ctgcgtaagc ccatcgaata gtcgttcccg acgggctagc
    15121 accgcgtcgc gaatccgagc gtgcaacgcg atgccctttg cggtgagcga aaccaacagc
    15181 ttgcgaccat cgtctcggtc cgagtctcgg tggagcagcc cattgcgctc catggtcatc
    15241 agcatgcgcg tcaggttcgg gcggtcgatg aagaccccgt cacagagttc ggtttgagac
    15301 tgcccgggtt gctccgcgag cttgttgaga atgaaccact gctcctgcgt agcctccaag
    15361 ccgcgctccg ccatcaggca cgcgaaatcg taccggagca gacgagcgct ccggtagacc
    15421 aagtaagcga atgagctctc caaacgcacc gcgcggggaa ccacgttgtt catctttggc
    15481 caataccatt ctggaggggt gagttcatgg cgtcacacat ttgggacccg aatgggagac
    15541 accgttttgc acgaaagctc cgctgcgtac tgaggcgtca ggacgtgggc cgcgggtcga
    15601 aacgcccaga atatcggaga cggattcaag cacctggtca cggtaatcgt ggatgaagaa
    15661 atgtccgccg tcgaaccaga ccacccggct cggcccagaa gtggcatcgg cccaagccgt
    15721 cagtgcctcc ggcgaaacca gaccgtcctc gaggcccccg aacacggtaa ttgggcactg
    15781 caaacggggc cctcgggccg aaacgtgctg ctccgtggcc ttgagatccg cgcgcacgac
    15841 cggcagtagc gcggccatca aatccggatc cgcgagcacc gcctccggcg tacctcccat
    15901 cgcgcgtaga gcgccaatca gctgcgcgtc gggtaaccga tgcagcgccg gtgtctccaa
    15961 cgggaaagac ggggaacgac gtgcggaggc gatcaaatgt tgcggcccca acgaggcggc
    16021 tccttcgagc accaaggcga gctcgaaggc gatgatcgcg cccaaactgt ggccgaataa
    16081 cacgtagggg tggtccacca acggaagcaa agcttcagcg agcctcggaa ccaaggaact
    16141 caccgaatcg aacgccggct cgtccatccg ctcttgcctg cccggcagct gcacggcgat
    16201 cacctcggcg accttggaca ggtgcgccgc ccagggtcga aagacgccgc tgcccgcccc
    16261 tgcatagggg atgcagatca gtcgtggcaa gctcggcgag ctcgcttgga agcgctgata
    16321 ccaagggctg ttcattggac tattcatcct attcatcctg agccccgccc aaagtgccgg
    16381 ccacacgcgt cactgacgtc gaggtgccga gttgttgtaa cgttcgagcc agcagactgg
    16441 ccgtgtgctc cagagccggc ggcctgagaa tcgagtaatg atccgctgcc acaccgaaat
    16501 cggtgaactt tccggtcacc caggctcccc agtccgtgcg aggggtcggc tcggccatgc
    16561 ctagctcgct cgaatggggc tggctaggag cctcgagttg cgtaccgtac tgggcccaaa
    16621 tatgggtgac ctgccccggg tacgaagacg ggctgtactt cgctagcgcc tgcaggcccg
    16681 ccctgtacac ctcccaaagt ggccggatct gctcgagccc gacgtgctcc ggcaaaagcc
    16741 ctgagctctt ggctagggtc aacgactgga gcatgagttc ttccagatcg caggctcggc
    16801 ccagcgctac tgggtccaat actctggccg tgtccgctcc cgcgagacgg ctcaagtcgc
    16861 caaagaagcc gagtagcata cgccgctcat cgctctcctc gcgacctggc acggggagca
    16921 ccgaatcaat caacgtcagg gagcttaccg catagcccgc gaaggagagt tgtcgtgcca
    16981 tttcgaaggc aaccacaccg ccgagtgacc accctgccag gtgaaccccg tggtcgcgga
    17041 gtgcgcgccc atcgtcgcgc gaagtgatgg atcggcagta agcttccgcc atctgctcga
    17101 tgctcgttag aggtgacgag ccccgcgcca agccgtgggc ctgcaaacca tgaacctcga
    17161 actcgtccgg caacagctgc gccagatcac gatagcagag cacgttgcca cccacggggt
    17221 gcaccaagaa catcgctttc ggttggctcg tacccctcgc tgcacgcatc gggattaggt
    17281 tggtcgcagt gggttcgtcg gaaggcccgc ccaatagcat tgccagtgca cggatggtgc
    17341 cgtgttggaa caaggtgctg agcggcagct cacggcgaaa ttcctgcttg atgcgcagca
    17401 tcaaccgcat tgccaagaac gactgcccgc ccgcatcgaa gaacccatcc tccaccgaca
    17461 agtcctctcg ctgcagaata tcctgccaga tccgaagcag cttggactcg agctcattac
    17521 tcggccgcac gatgcggcgc gtggcgacct ccacggagat cgagcgagcc agcgaccctc
    17581 gatcgacttt gcccgtcggc gtaagcggca gttcgggcaa gaacacgaag cgagacggaa
    17641 tcatatacgc cggaaggcta cgggccagag cttctgacaa cagcgcactg gtcacttcga
    17701 gctcgggctg aactacgacg tacgcgacga gtgtttttgg tccgctccgt tcacccaccg
    17761 cgctcgcggc cgccgcgcgg acatagggca gcgcggcgag cgcactctcg atctcgccga
    17821 gttccacccg gaagccctga atcttgacct gattgtcact gcggccaaga aactcgatct
    17881 ctccgtcgct cgtgtagcgc cccaagtcac ccgtccggta gagacggtct ccggttctcg
    17941 gatggtggaa gaagcttctt tgggtacgtt cgggatcgcc gaagtactcg cgagccaggc
    18001 cgatgccccc gatatacaga tcacctggca cgtaggtggg gcaaggttcg agtcgttcgt
    18061 ccagaacgtg aaaggattga ttctggagtg gacgaccata aggaatgctg cgccattccg
    18121 gacgcacatc gcgaatctga tggtaaattg accagatcga ggcctcggta gcgccgccga
    18181 ggctcacgag ctctagctcg ggcgcaatcg agcgcagcag ccccggcaga gaggtcggga
    18241 tccagtcacc gctcagcatc acgaggcgca gcggatcgag agcctcgctc gacgccaccg
    18301 cggcctcggc cagcatttgg gccagcgcgg ggaccgaatt ccataccgtc acgccatcct
    18361 ttttgatccg atgcagccaa cgttgtggat cacgcagctc ctccggagta ggcagcacca
    18421 aggccgcgcc agcagccaag gcgccgaaca agtcccaaac cgacaaatcg aagctgagac
    18481 tcgaaattcc gaacacccga tcggtctcgt tcagtccaaa gcgcgtgttg atatcccgga
    18541 tcgtattgaa cgcgcccagg tgctcgatga cgacgccttt cggattaccc gtcgagcccg
    18601 aagtgtagat gacgtaggcg atatcgccgg gcttctggcg acgtagccag cggctggcgg
    18661 gtggcagcgc catcccgctc cgcgacggcg aagcctcgcc gacgacgacc ctcgacacgt
    18721 ctgcggacca attcagggcg gcatcgctct gcgcgtccgt aacgacgaga cgcgctccgc
    18781 tgttgcgcag catgtagtcg acgcgatcct gagggagctg cgggtccacg ggcacgtacg
    18841 ccgcgcccgc gagctgcacc cccagcaccg cgacgacctg acgccacgac ttcgggctca
    18901 ccacgcccac gatctcgttg ggctgcacac cgcgctggat caactgctcg gccagcgagg
    18961 ccgctcgtgc gtacaactcc gaataggaga gcgtttggtc agaaccaatc accgcggggg
    19021 cgtccggctg cgcttcgacg cgcctcagga acggctcgaa caagcactcg gggaggcact
    19081 cggcgtgtgt cgcgttggct tcgcgccggg ggcgaagatc ggagtcaggc accattccga
    19141 agcgcaccac ggcgtcgaag ctcttcgggt cactcgccaa acgcacgagc agcgagcgat
    19201 acacgccgaa cagcgtttcg accaggggcg ccgggaacgc ttcctcgagc acatcccaat
    19261 tcacgatcag ctcgccgtct tcttcgagca cttgatggtc gagccagacc tgcggagttc
    19321 gaatcgaaga gtgcacctcg cggccctctt ccgccatgga gagcaggtgc cgcgtgagcc
    19381 cctcgcccat ctcctgcttc tcttgcccac gcaacgtgct cgcgaagacg accggcatgc
    19441 tggcctgccc gccctgtcca cgcgaacgat tgagctcacg caagacctct accccgctca
    19501 cggccgcatg ctcgagatcc cgggccagcc catcccgcac ccggcgcgcg ttctcccgaa
    19561 atgcccgcgg ctctctcaag tcgatctcga gcagcacggt ggtactgaag ttgcccacca
    19621 ccgacttgac ctgctcgtgc gcgggcgctc ggttgaagta cagcagattc agcgtgaagt
    19681 gcgcggacgc gctaaaacac gcgagcacct ccgcgtacgc cgtgcagatg gcgacggatg
    19741 gggttacgcc tgcctcccgc gcggcccgct tgaatcgctc ccaacgcccc gcgtccaagc
    19801 ggaacgtgtg atgcgcgaac gggacgcgcc cctgcgcgag ccgggcgcta tccacaggca
    19861 actgtggcgc tggcgggaga gaccgcacgc gctctcgcca atagccgagc gcggcgcggt
    19921 agcgctcgga gcttttctgt cgctccagcc aaagcacata gtctcgatag ttgagctcca
    19981 agggctcgag cggcacgaac ggatccgcat acagctgcgc ccactcccgg aacagaatcg
    20041 aggtgctcca ggcatccacc gccaaggcgt cgaaacatac atgcaggcgg atccagtcgt
    20101 cgcggcgcag cgtcgcgcga atttcgaaca gcggccactg ctcgcatggc aagacgcgcg
    20161 cccgcagcag cgctcgcgta ttcgcgagct cgcgcgcttc gccctccgcg gactcggagc
    20221 gcaggtcact cacgcggatg tgatagcggg gcacgtgcct cgcaacctga ttgcgaccat
    20281 cgctttggat ggtcacacgc agcatcgaat gtcgatgaat caactggttc aaggcgactt
    20341 cgagccgctc gagatcgatc cctcgcagat cgagctccag gtaaaacgcc gtggaggttt
    20401 gcccgagctc gaagctcgag ccgcgcccga gccaatacgc ttgctgcatg tccgtgagcg
    20461 ggaatggctc attggcccgc gaccgatcgg ggacgatgcg gtcgctcgtt tccaactcaa
    20521 aagccgcggg cggcgacact tgcgagagct ccccctcggc ggcgcgcggt atcgtggaca
    20581 aggcgtgcga ggccgctacg cggggctccg cacggggcgg tgccggcgag accggcccgt
    20641 ccaggcttgg actcgcgctc tccgagcctt cgacttcaca cagcgactgc gccagttcga
    20701 gcagggtcgg gctctccagc aactggacca agctgggctc gagcccaatc gatgtctgga
    20761 gttgcgctct caactccacg agcatcagtg aatcgagtcc gagttccagc agcggtgtct
    20821 cttcgtcgat cgtgctcgga gccgcgcgca agattgcgcc gagttgctta cgcagcgtct
    20881 cacggaccca ttgcagccgc tccgcgggcg ccagagcgga atggctttgg accggacttt
    20941 gccccatcga gggcgccgca ggcgcgggct cgcttcgcgg tgcggctgcc ttctgtccgt
    21001 cgctcgcgtg cgcggcagcg ggacgaagga gcgcgccccg cgccaccgag agcactgtac
    21061 cccgttcgtc gtagacggtg atctcggcct gaatgccggc ctgcttggat tgctcccgcg
    21121 gaggccgggc gaggcgcgcg cgacaatagg caggacgccc gccgcggctc gggcgagcga
    21181 actgccgcac gcccgcaacc atgtagttct gactgagact atcgtcgatc atcgcgtata
    21241 cgagctggaa cgcggcgtcg gtcacgcccg gatgcaactc gcagctcacc cggtcatcct
    21301 ccggagttgg cggacgtagg cgcgcgactg cctcgtcccc ctcggcgttc gtccagatct
    21361 cttcaagcca ttgcgccgcg ggcccgagct ggatgccccg tcgccacatc ttggcgtaaa
    21421 atctggagcc cgccagcgtc gccgcggcac cttgtcggaa ggcctgcagg tccacgggag
    21481 ctctctcggg tgcaacgcca gccctgcgcc ctttgccggt gcagtgcaga gaccaagttg
    21541 cggcgcgctc tggctcgcct tgattggcac tctgttccgg ctcagttccg tagatggcca
    21601 aatcgaaggc ctggtcgccg cgcggagtca gcacgacatg cagatgctgt tgctgctccg
    21661 ccgcaatcat gggcttcatc accgcgaact ccaagagctc cgccggccca cccaaagcct
    21721 cggccaatgt ttgaaaggcc acctcgacga acactccgat attcaccacc ttcgcgttcg
    21781 cgatcaggaa gtcgcccaag aatgggaatt tgaccgggct gagccgcaga tcgtactgca
    21841 gaatatcgag cggtgacgcc acacgctgcg cggccagcac ggctgagcct gcttcccgtt
    21901 cggccccctt ttcacccgcc gctccggcgg cagttcctac gtgacccgca ctcgcgccga
    21961 gttcggaggc tggctcggca ccggcctcca cttgcgccga aggtcctggc cagaatcgct
    22021 gcctctgaaa cgggtagctc ggaagcggag cgcgccgacg cgaaagccct gcgtcgattc
    22081 ctgtccagcg cagttgggtc ccgagcgaga atagctgcgc gagtccggtc atcatcactc
    22141 gcgcatcgga gtgtccgggt cgcaaagacg gaatcagggt cgtgtctccc tgcgccagcg
    22201 gcttcaccag gctcgcgagc gtcggctttg gacccacctc gatgaacacg ctacaccctt
    22261 cctctagcgc cgaacgtgca cactccagaa actcgaccgg ccttcgcgtg tgagaaagcc
    22321 aatactcggg agtggcgatc tccgcccccg cgacgccacc gctgacgttc gacacgaccg
    22381 ggatcttggc cgcgccccaa gccacttgct cgagctcgcg tcgaaactgc ggcaggatgc
    22441 tgtccatcaa cggcgaatgg aaggcctgcg aaaccgcgag cgccttggtc ttgatgccct
    22501 gcgccccaag ctcgcgaatt agctcttcga gtgcttcgcg tgccccactg agaacgacct
    22561 gcgacgggcc gttcactgct gcaatcgcca gctcgccttc gtgaccggcg agcagcggct
    22621 ccaggctctc gcgcgaggcc acgacgctga acatcgcacc tcgctcagcc acttcggaca
    22681 tcagccggct gcgcgcggca atcaggcgca agccctgttc gaagccgaac acgcccgcga
    22741 tggccgcggc tacgtattca cccaagctat gccccaataa caggctcggc tccaccccgc
    22801 aatccatcca gaagcgagcc accgcatact cgagcgcgaa cagcgcgggt tgggtgtaca
    22861 gcgtgtcgtc cagcagagca gcgttgtcgc tctgcgtctc tgcgctaaac atgacgtcga
    22921 gtaggggcaa tggcaaaagc gggcgcagaa cctcgtcggc ttgctgcaga atcgaacgaa
    22981 cggaaggctg atgctcgaaa agctcacgcc ccatccgcac gtattgagag ccctgccccg
    23041 tgaacaggaa cgcggtccgc ggcttcgcag agcccttcac gcgccgctcg aagctgcccg
    23101 ccggaagttc accttcacgt cgcttcgcaa gtacgtcggc gagttcctgc acgtcagcta
    23161 cggcggtgga gaagcgacac gcaaaaggag cgcggctctc tccagccgtg aagcagaggt
    23221 cggccaaatc gagccctcgg ttctcggcca agtgggcctg ccagcgctcg agcagacccg
    23281 agagggctcg ttcgttcttc gccgagaccg caaatacgtg ggccggccgc gcgcgggttg
    23341 cgggctcgcg ctgagaagtg gcagtcgggg cctgttcgac gatcaggtga gcattcgtgc
    23401 cgctggcgcc gaagctgctg atcgccgcga ctcggcgacg ctcggtgtcc ggccagctct
    23461 tcgcttctcg cgtcaactcg gcggggatgc gctccaaccc gagatttgga ttcagctcgg
    23521 tggcgtggag atgcgatggg atgtgaccgt gctgcaagca cagaatcgtt ttgatcagac
    23581 cggctatacc cgccgctgat tccaagtgtc caatattgga cttgaccgaa ccgaccaaca
    23641 aggggcgatc cggggcccta cctgttccgt aaacgtccaa ggcgcccgcc tcgatcggat
    23701 ctccgagtgg agtgccggtg ccgtgggctt cgacgtagtc cacttgattt ggcggcaccc
    23761 cggcgttccg cagcgccgcg cggatcacct ctcgctgcgc cgttgcgcta gggacggtca
    23821 tcccgctcgt gcgaccatcg tgattgatgg cggagccacg aatcacggcc gcgatggggt
    23881 ctgcttcggc gagcgcgtcg ctgagccgct tcaacacaac tacaccgcac ccctcaccac
    23941 gcgcgtaccc gtcagctcga gccgaaaacg gcttacaacg cccgtccggc gccaacgcgg
    24001 ttccgcggca caatacgatg ttccattctg gcgcgagcac cagattggtg ccaccggcca
    24061 aagccgcgtc acattccccg ttgcggagcg cgttgcacgc caagtgaatc gctacgagcg
    24121 aggccgaaca tgctgtatcc acggccaacg ccggccccgt gaatccgaaa gcgaacgcga
    24181 gcttccccgc gacgaagctc ggtagggtgc cagttaggta gtgcaagtcg agcgaggaaa
    24241 gaccttctcg agagagcaac agaggcccgt actctcggcc cgaaatacct acgaacaccc
    24301 ccgttcgccc gtgttgcagg cgcggcaacg attgaccact acactcgagc gcttcccaac
    24361 agacctccaa aaaaaggcgc tgctgcgggt ccatcgcggc ggcctcgcgc ggggagatgc
    24421 cgaacgcacc cgcatcgaag tcctcgatgt tctccagaaa ggacccccgt cgggtgtaca
    24481 tcttcccgac gcgcccgggc tcactcgagt agtaggcttc cgcgtcccac cggctgcgcg
    24541 ggatctcgcc gctggcatcg taaccggcgc acagccgacg ccagaactcc tcaggcgagt
    24601 tcgccccagg aaaccggcag cccataccca ccaccgcgat aggttccgag ttgcgagact
    24661 tgagctcctc gagctcgcgc ttcaatcttc ggatttgtac gagcgagcgg gtcagcagct
    24721 cttgctgcga aacatcgcta ttgagcgcgt tggtttccat attcgattcc gtgcctacgc
    24781 cgtgttcccg tgcgttccgc ttgtgtactg cgcttgttcc tagaccgtgg cgttcgtctc
    24841 gagctccgcg agctccattg ccaaggatct gagtaagtcc ccctcgtcga gcgtcagcgt
    24901 cgcctttcgt ccggatgatg cggatggcgc cgccggtgag gagagtgcga cggttgggga
    24961 gtctgggacc ttcagcccgg atgacttccc ccggttgtct ccgacgcttt ctccggcgct
    25021 ccgctcgagg tcgcccggtc gagagtcgtt cgacagtcga gcatggagat agtggctcaa
    25081 cttggacaca ctcgggtact cgaaaacgac gcgcgtcgcc agcgttagtc cgagcaactc
    25141 ggatatcctg aggtgcaaac gcacggcaac gatggagtcc atgcccattt gaaagaaccc
    25201 ggcgtcgcgc tccacgtttt cgggatccgc tcccagcgca cttgcgacct ccgaaagcac
    25261 tgaatcctcc aacagcgcag cccgccgatc ggcgcgcgca ctctgccaac gcagccgtag
    25321 tgagcccacc gccccggcgc cgccttggct cacgttggtt tggttcgcac ggcttgggtt
    25381 taccgcgtct tggggtgcgg tggcgacagg aaggcggtcg agactcggta tcgattgacc
    25441 cgccgcgggg cgctccttga accagtatgg gcggtgttgc catacatagc gaggtaaacg
    25501 ctcatgagcg accggggtct gccgctcgcc gagcgcccgt gaactgagcc ccagtacgtg
    25561 gagctgcgcg cgcgccaaag cgagagactc ttcgacttgg cgatcacgat gcaaagtagc
    25621 gagcgcggcc accgttttgc cttcgaactc ggcggcctcg atcatgggcg tagtgagaat
    25681 cggatgtgga ctcacctcga tgaatacttc gtggcctgcg ttgagcagcg cacgcatacc
    25741 ctcgaggaat cgaacccgct ggcgcagatg ctcggcccaa tagttcgcgt ccatttcgga
    25801 gcccgtgagc agctggcccc gcagggtgga ctgaatgggg atactgccgg cgcgtgggct
    25861 caccgactcg agctccgcgc gcaacggagc catcaatggg gccgtttgcg ggctgtgaga
    25921 cgcgaaatct accctgattc ttcgacagaa gacattgctt gccgtgagcc gggcatggag
    25981 cgcatctatg ctcgaagcag cccctgaaag caccgtggag acgggaccgt tctcggcggc
    26041 gaccgccacg ctatctcgga attcccccag cgcggcctcc agtttgtcga cactctccgc
    26101 cacgaacagc atgcccccgc cgcgaatagt tcgtgccagt tcgctgcgct tgcagatgat
    26161 gcgtgctccg tcctgcaggc tcaatgcgtt cgcgaccacg gccgcggcga cctcgcccat
    26221 gctgtggccc agtacggcgt ccggcgaggc gccccagctc tgccacagtc gcgcgagcgc
    26281 gacttgcatc gcccacagcg caggctggag gacggtaatg tcatcgaggg cgctggcatc
    26341 cgagctcgtg atttgctcga ccaaagacca actcacgaac tctcgtagtg ccgcctcaca
    26401 gtcctgaatg gtctgccgaa acacgggttc tcgctccagc aacgcgcggc ccataccgag
    26461 ccattgggag ccttgccccg aaaacacgaa gacgaccttg cggctttcga cccctactcc
    26521 cccgctcacc acgcaggggt cctgcacacc cgatatgaat cgcgacaggc gctcacccat
    26581 ttctgatttc gtgcgcgcaa ttaccaccgc ccgctgctcg tgctgcgtac gacgttgcgc
    26641 cgcgctggca cagacggcgg gcagcgggct cggatgcgtg gcgagaaact cccggtagct
    26701 tgcggcttga gcgagcagcg cgtcgcgcgt tttaccggag agtggcaaag aatacgtcgc
    26761 cgacacctcc tccgtgggaa tcgagggctc cggcgcatcg tattcctcca aaaccacgtg
    26821 ggcattcgtg ccgcccgcgc cgaatgcgct gaccgcgccg acccgagtgc caccttccga
    26881 tcgccaaggc atacgttctc caggcacgaa gaagcgcgag cccgcgaggg gtacttccgg
    26941 gtttgcctcc tggaagtgca ggtgcgcggg gatctctcta tgcttcaagc acaacgcgag
    27001 cttgattaga ccggctacac ctgcagccgc ttccaggtgg cccaagttgg tcttcaccga
    27061 tccgagcgcg cagggtctcg cgttctgctc acgtgaggaa tccccagccg aatagacgtt
    27121 cctcagcgcc tcgacctcga tcgggtcacc gagcttcgta ccggtgccgt gcgcttcgat
    27181 gcaggatacg cggctgggcg ccaaacccgc atcctgaagg gcgcgacgaa tcacgcgctc
    27241 ctgggcgagt ccattgggcg cagtgagccc gttggtccgc ccgtcctgat tgacggcgct
    27301 ccctctgatg agggcccaaa tcgcgtcctt gtctcgaatt gcgtccgaca agcgcttgag
    27361 tacgaccacg ccgcaacctt cgccgcgcac gtatccgtcc gcgcgcgcat cgaaggttct
    27421 acagcgcccg tccggcgcga gtggataggc tttcacgtac gcgtgcgtcg cgtgcgcaga
    27481 caggatcaag ttgactccac ccgcgagcgc gaggtcactc tcccctgatc gcaagctctg
    27541 gcatgcgaga tgtacggcca cgagcgatcc agaacacgcc gtatcaatcg ccaggctcgg
    27601 cccctgcaag tccaaaagat aggagagccg attggcgatc atgcagtgca caccgccgtt
    27661 gatggtgtag atgtcagcca aggcgggctc gagcaactgc atgtgcaggt agtcgctgtt
    27721 gacgacgccg aggaacacgc ccgtgttcga gccgcgcagc gcctcgtgcg gcaggcccgc
    27781 gtcttcgatt gcctcgcagg ctacctccaa gaagatccgt tgctgaggat ctagttggcg
    27841 cgcttcacgc ggggagatcc cgaaaaagcc ggcatcgaac ccggccacgt cgtccaaaaa
    27901 ggcgccaaag cgaatgaggg aagagcgatc agcctccacc gggctaccgc cgacgaagcg
    27961 atcctccggc gtcgctcgaa cggtatctgt tcccgaacag agcaagttcc agtaggactc
    28021 ggcgccgtac accgaacccg gaaaccgaca gcccaaaccg acgatcgcga tcggctcgct
    28081 tcggcttttt ttccaggact cgagttgatc gcgcgcggct ttgaccgccg ccgttgcccg
    28141 ccgcaggata tcctggttcg acttagacat ttcctgaact gacatggctg tttcctgatt
    28201 ggctatcgag aagctgttcc agagcggcga gctcccgcgc gaggtcgctc tcgacttcgg
    28261 aatcgcgttc ggtcggagcc gccgtactcg tcgtctctgg cgcgcgagcg gagctctccc
    28321 cctgcggcgc ggactcgcgg ttacgaagat cgagctgccc ggccaagtac ccgcaaaagc
    28381 gcgacaacgt cggataattc caaatcgtcg cggccgagaa gcgtaacccg agcgagccct
    28441 cgaggcgatt gcggagttcg agccccatca atgacgtcat acccaaatcc cgaaatgggg
    28501 tatcactgcc aattcggctg ccctccaggg cgagcacaat cgaaagagcg gcgcgtacgt
    28561 ggcccagcaa cagcgccttt tgggcggctt cggaaggcgc ttggcgcaac agttcgcgaa
    28621 agttcggcgt cgcattgggg gcgcccgagg ctcctgccga cgtcgtggtg cgcgtgccca
    28681 cgctatctgt ccgagcatcg tgaacagagg ccgtgcccgc tccggcgccg ccgcgagcct
    28741 ggaacatcga caccggcacc ggcacgagct cgatatgatc gagggacagg cacggccgcc
    28801 ccgcatcatc caacatcgct agcgcggcgt gcacgttagt tggcggcgcc gccgcgcgga
    28861 gggtgtccgt gtggttcgcg ggcgtactgc ccgagaggac gcgtgcttga acgatggccg
    28921 cgctgttcaa ttgggcgcag ttcgatcgag cgctgtccga tccagaccag agacgtcccc
    28981 gaacgatccg tgagggaacg aggtattgat ccagtcgctg cggcaaacag agggccagcg
    29041 cctgccatgc ggcctcgagc gccaagggca cccgaatgtt gttcgacgcc gattcaacac
    29101 ctactcggcc caacgcgcct tccgggctga gctgcaaacg agtaagaacc cgcaatcgct
    29161 ccccgcagtg caggccccgc tgtgcgagac gcttatacag tgcgtcgcct tcgaggcggc
    29221 attcctcttg ggcccaagct cccgagtccg tgctcgaatc gatttcgctt cgagcgggca
    29281 acaagccagg tggaccggct tgccccgtga agacaacgtg ttcacaccac tcgtcggtct
    29341 cgaggcgccg tgtgtgcagc cagacgccca ccttgcccgg cgacaattcg cgagccatca
    29401 tttgcgcctc gagcgctccg gatccctcga ggcgcaaggc ttctttgaat tcaatctgaa
    29461 agtcgattga tccttccggc aggatagagc ccgccccgta tagagcccac tcgagccaga
    29521 acgacgcgga gagcagcgga atctctccca attggtgttg ggcgagctca ggcgcgcgcg
    29581 ctggatcgat gtgaaacagc cacaatctgg ggacagagag gcgcgctccg gttgccgcgt
    29641 gcggcccgct tatggcctgg gcacctgttg ataggaggga ttctgccgct ccgtgggcca
    29701 ttgcgggcag ttgcatgggg ctcgatcggg agggttcgag cgcccgttcg atgcgagccg
    29761 tcggggactg cgcttcggat tcgatccagt agcgctcgcg ctgaaaaggc agagtaggca
    29821 agagctcgac ctggccccct tgccgatgaa cccagtccca gcggacgctt gctccgtgcg
    29881 cgtgcaatgc gcccagagtc gctagtaggg tcgcgtgctc tggctcgtca cgccgggtgc
    29941 aaccggtcac caacaccgcg ccgtgctccc ccgctccgcc gtgctccccc gccgcacact
    30001 gctgcagcgc gggcaacagc accggatgag ggctgatctc gaggaatcga cggtggccct
    30061 ggcgcaagag cgcgcccacc accggcgcga accgcacagg atcacgcagg ttgcgaaccc
    30121 aataatgcgc atcgagttcg aggcccgaga gcagttcgcc acgtacggtc gacaagaacg
    30181 gcaccttggc tgatcgcggc ctgaggtgcg cgaggctatg ctccaaatcc acgagcaact
    30241 cgtcgacttg ggggctgtgg gaggccacat cgacgcgcac ccaacggcaa aacatagttt
    30301 tcgccaggag ctcgcgctcc acttcccgaa gtgcggactc cgcaccggag agcacgcaag
    30361 accgaggact attcagcgcc gcgacggcga ccgcgggcct ccccgcaacc aatgcctcgg
    30421 cctcggcagg ttcgagttcg gtgagaagca tggcgccccg cccgctcact cgatccaaca
    30481 ggcgcgagcg aatacaaatc acgtccactg catcttcgag gctaagaaca cccgagacgt
    30541 gcgcggccgc gacctctccc atgctgtgcc cgaccaccac cgccggttcc accccgaagc
    30601 tgcgccagag ctcggcgaag gccacttgaa acgcgaagat cagcggctgc acgaacctcg
    30661 accgcttcag gtcctcttca ctcgcctggc tcagttcatc gagcaggcgc cgaccgtgac
    30721 gctcgtgcgc gacccgtgcg acgcgctgga gagcacgccg aaattcgggc tccacttgcg
    30781 tgagctcccg ccccattccc aaccactgcc caccctggcc ggggaacacg aaagccacct
    30841 ggccattcgt cagctctgcg cgaccgcgcg cgagccctct caccggcgag ccgaactcga
    30901 aggcctcgag ttgatccgcc agctcacccg tgttgcgggc tacgaacgag acgcgctgtt
    30961 gctggtgatc gcgccgggag ctcaacgtgt acgcgaggtc actggccacg tcccgggtcg
    31021 ccttcctcaa gtgctgcgcc aactctttcg cgcgctgcac gagcgcgggt tccgaatgtg
    31081 cactcagggg gaagcagaag cgccgcacac tggcgctccg cgcgcgagcg aggcgagatt
    31141 catcgctctc tagcccatga atctgctcga tcgtacggag caggccgcga gattcttcga
    31201 gctccttcgg cagaggcacc aggacatgac ctgagccttt gagggcctgc ttcaattcgc
    31261 gggcaagcac cgggcgtgga ctcagctcga tgaaatgtga gtatcccgca gctatcaact
//
LOCUS       DASTKU010000950         2915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_186702, whole genome shotgun sequence.
ACCESSION   DASTKU010000950 DASTKU010000000
VERSION     DASTKU010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186702"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="VFQ61_33720"
     CDS             complement(<1..1592)
                     /locus_tag="VFQ61_33720"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET9959515.1"
                     /translation="MSFSCWRPRLMLMAAGLLACAGAAACDSDRRSEEPASNGGQSGG
                     QSGGQSGGGGAQPAGGDAGAPSRDRRYLSGLQTMDDYVALAGPGAEVKYLIASQDDAW
                     PTPFAATRCLFQNTRRYPYHLQFLRTFPEYAQLSPDAYEDLVLRQASRVMWGGGLKLF
                     AATMHPSTGMPGVIAFTVYAEESPSERLSAAQVALVAEVLSGCAPFLAQRLVWIPDGS
                     AQLARFESLQGPLSELGVVAVHPAALRAGLSAESYSVGEAYGYLAVLTSSVAAAAVSP
                     QDVLITDSAPNDLSLVAGLLTVQPQSVASHLNIRLREQGVPSASVPSILENPLILELA
                     NQLVHVVVTDDDVQIEPAPLEDAQAFWETNRPYVGQPEANLEASEFLPFDTLRASDSG
                     VFGTKAANLGELHSVLSAGNRVEGFAVPFRAFSEFLRDNGLDGAVAELLLDEQVRIDS
                     AYARSRLANLRRAIRAAPVPEHLQAALHAAVEQAYGAAGLTTRLRFRSSTNAEDLPGL
                     SGAGLYDSASGCIADDLDADTDG"
     gene            1723..>2915
                     /locus_tag="VFQ61_33725"
     CDS             1723..>2915
                     /locus_tag="VFQ61_33725"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET9959516.1"
                     /translation="MTATLRNSRRVHGQRTSTVQTCASEKRTAIRSTRSRCAPIPKRG
                     LIAGALALGLIWGCSSEEAPASPPATTSSGGDAGSSNEGGAPSNTGGDNAHGGTATGN
                     SGGTAPDPGGAAGSGGASGFAPIPGVERGDELCGSAPGGDIYGKHVLRFENADAAITV
                     QIGRTYLGPGVGESSIYGLTDFSLLIDGVEHCSGDPGDITETGYQNTHHNWQDLATAT
                     LETVHYELFVGMDFSEDETLRWAPTLSAVDRDSGETLLDGLPLTATGGPASCWTCPSS
                     VPVLITEVLAHNESTVVDEQGEYEPWIELFNPSSSSVNLAGWTLSNDLADRTRWRLPD
                     VMIPRHGYLLVFADAQPAQGELHANFRLSPEGGAIILTTADGVTNGGQAFGPQAPDQS
                     FGFTF"
BASE COUNT          558 a          972 c          907 g          478 t
ORIGIN      
        1 ccatccgtgt ctgcatccag atcgtcggca atgcagccgc tcgccgaatc gtagaggccc
       61 gcacccgaga ggccgggcag atcctcggcg ttcgtactgc tccggaaacg cagccgcgtc
      121 gtgaggcccg ccgccccgta agcttgctcg acggcggcgt gaagggcggc ctgcaaatgc
      181 tcgggaacgg gcgcggcgcg gatggcgcgt cgcaggttcg cgagccgcga acgtgcgtaa
      241 gcggagtcga tacgtacttg ctcatccaag agcagctccg ccactgcgcc gtctaggccg
      301 ttgtctcgca ggaactcgga gaacgcacgg aaagggaccg cgaaaccttc gacccgattt
      361 cccgccgaca gaacggagtg aagctcgccc agattcgcag ccttggtgcc gaacacgccg
      421 gaatcactcg cccgcaacgt atcgaacgga agaaattcgg acgcttccag attggcctcc
      481 ggctgcccga catagggccg attcgtttcc cagaacgctt gcgcatcttc gagtggtgcc
      541 ggctcgatct gcacgtcatc atccgtgacg accacgtgca cgagctgatt ggcgagctcg
      601 agaatcagcg gattttccag gattgacggc accgacgcag aggggacccc ctgctcgcgc
      661 agccggatgt ttagatggct ggcgacactt tgcggttgca cggtgagcag ccccgcgacc
      721 agagatagat cgttcggggc cgagtccgtg atcaggacgt cttgcggact caccgcggcg
      781 gccgcgacgc tggaggtgag gaccgcgaga tagccatatg cctcccccac gctgtagctc
      841 tcggcagaca agccggctcg aagtgcagcc ggatgcaccg cgactacacc aagctcgctc
      901 aaaggcccct gcagcgactc gaaacgagca agctgtgcgc tgccatctgg gatccagacc
      961 aggcgctgcg ccaaaaaagg agcgcagccc gagagcacct ccgcaaccaa cgccacttgt
     1021 gcggcgctca ggcgctcgct tggagactcc tcggcgtaga ccgtaaacgc gatgaccccc
     1081 ggcatcccgg tactcgggtg catggtggcc gcgaagagct tcagcccacc accccacatc
     1141 acgcggctcg cctgacgcag cacgagatcc tcgtaggcgt cggggctcag ctgggcgtac
     1201 tcgggaaaag ttctgaggaa ctgcaggtgg tatgggtaac gtcgggtgtt ttggaacaaa
     1261 caacgtgtgg ccgcgaacgg tgttggccac gcatcgtcct gcgaggcaat caggtacttc
     1321 acctccgcgc cgggcccggc gagcgccaca tagtcatcca tcgtttgcag gccggacaag
     1381 tagcgacggt cgcggcttgg tgcacctgcg tcgcctccgg cgggttgagc gccgccgcct
     1441 ccgctctgcc ctccgctctg ccctccgctc tgccctccgt tcgaggccgg ctcttcggac
     1501 cttcgatcgc tatcgcaagc ggcagcccca gcgcatgcca agaggcctgc agccataagc
     1561 atcaaacgcg gccgccaaca cgagaagctc atggatcgaa agtccgccga cagatcctgc
     1621 tgtgccccag ctggagctgc tcccctggag tacctcgaaa caagcaagcc ttgtgccccg
     1681 tgcatacgcg gaaaggcacg tggattgcgt tccgcgcgac ccatgaccgc gaccctcaga
     1741 aactcgcgcc gcgtgcacgg ccagcgaacc tcgactgtgc aaacttgtgc cagtgaaaag
     1801 cgaaccgcga tccgatcgac gcgtagtcgg tgcgccccga ttccgaaacg cggtctgatc
     1861 gccggggcgc tagctctggg gctcatttgg gggtgctcct cggaggaggc tcccgcgagc
     1921 ccgcccgcca cgacgagctc cggtggggac gctggctcgt ccaatgaagg cggagctccg
     1981 tcgaacaccg gcggcgacaa cgctcacggc ggcaccgcga cgggcaactc gggcggcacc
     2041 gcaccggacc caggcggtgc cgccggatcg ggcggggcct cgggttttgc ccccatccca
     2101 ggcgtcgagc gtggtgacga gctctgcggt agcgcgccag gaggcgacat ctacggaaaa
     2161 cacgtgttgc gcttcgagaa cgcagacgcg gcgatcaccg tgcaaattgg gcgaacgtac
     2221 ttgggcccgg gcgtcgggga atcctccatc tacggattga cggacttctc cctgctgatc
     2281 gacggcgtcg aacactgcag tggtgatccg ggcgacatca ccgagactgg ctatcagaac
     2341 actcatcaca actggcagga tctcgccacg gccacgctcg aaaccgtcca ctacgagcta
     2401 ttcgtcggta tggacttctc ggaagacgaa accctgcgtt gggcgccaac gctcagcgcc
     2461 gtcgatcggg attcgggaga aacactgctg gatggcttgc cgctcacagc caccggcggg
     2521 cccgcttcgt gctggacctg cccgagcagc gtcccagttt tgattacgga agtgctggct
     2581 cacaacgaat ccacagtcgt cgacgagcag ggcgagtacg agccgtggat tgaacttttc
     2641 aacccctctt ccagctccgt caacctagcc ggttggaccc tgtcgaacga cctggccgac
     2701 cgcacacgct ggaggctccc ggacgtcatg atcccgcgcc atggttattt gttggtcttc
     2761 gccgatgcac aacctgccca aggcgagctc cacgcgaact tccggctctc acccgagggc
     2821 ggagcgatca ttctcaccac cgcggacggc gtgacgaacg gaggtcaggc gttcggaccg
     2881 caagcaccgg atcagagctt cggattcacc ttcga
//
LOCUS       DASTKU010000951         5412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_632424, whole genome shotgun sequence.
ACCESSION   DASTKU010000951 DASTKU010000000
VERSION     DASTKU010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632424"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VFQ61_33730"
     CDS             <1..1330
                     /locus_tag="VFQ61_33730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959517.1"
                     /translation="QTSPRTLTLDVGVTCESELVPRTPTRALALLDPLATSPLRFGVR
                     TAAPAPGEMLSFDLSARDEDSDGREDFRLNVSLAVGSTTPLASAPLVFLDRAAGVSRE
                     AREPARAINEALKAESVRAQRKKTAEAVLARVDATRRLLATLCAEGATPRVFDWNGDK
                     FTCGSLQDIVDRLGTLEVTAHSALSDWESALGALARDGWYFGKMRATARSALIKELQK
                     HLTRVPVTRVSLSTVPKATTPPSLSPLSFDSGGALWLETDGGLVRIAPSDTREEPVAP
                     DPSVSGWPVEPVGPRDERWISVSYSCDRSEVSLLLAGASDPTPAISGLLAPRPGVCAG
                     GRFDEAFAPAFIAAHDAREVFVGGQRFGDKKLGAPGPRGSARSPSGARVAIPSSLGLW
                     IQAEKSELWSVDNWDTPAARNCVVDDNARRAACIRGTRAELYLRNDSTQP"
     gene            complement(1516..2088)
                     /gene="efp"
                     /locus_tag="VFQ61_33735"
     CDS             complement(1516..2088)
                     /gene="efp"
                     /locus_tag="VFQ61_33735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET9959518.1"
                     /translation="MDTSDIRKGLKLMMDGQPYIVVDFQFVKPGKGQAFTRTRMKNLL
                     NGNTLERNIRSGEKLEAADVEERSLQYIYPEGDAFVFMNPQSGEQVTVQREAVGDDAG
                     FLIDGIEVSIIIYKGNPVSVTLPPHIVVQVTETEPGVRGDTATNVTKPAKISTGASVP
                     VPLFVNQGDWIKVDTRSRSYLERAKAPSGS"
     gene            2520..3374
                     /locus_tag="VFQ61_33740"
     CDS             2520..3374
                     /locus_tag="VFQ61_33740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9959519.1"
                     /translation="MEEYELEEIKREIVESRSLSIKTNNLINALAADLKSIAKRQQSY
                     ERRVFVNSATAYAVTIAVILVFVKLAWDIRLEAVRGESRETRDRVEQLEKELKNLQGR
                     EEALARVSRRASEFYQLITLNKRKELIEGFTEIAKLELSPTERSVFEAAVERARNELS
                     LIAYQNGLDHSRMGRFHEAQQSFRESLKYKTDAAHSPQANLQLSRALIKLGMHRDAIP
                     ILMQLSEASPDKEVMDEATLSLAFAQLDIQAWNDAKNTLRAFIRRFPSSVHINDAKGK
                     LAEVQLYH"
     gene            complement(3408..4706)
                     /locus_tag="VFQ61_33745"
     CDS             complement(3408..4706)
                     /locus_tag="VFQ61_33745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959520.1"
                     /translation="MSLVGALMSVGPVACGGSEPVQSAAASGGVESRGGNMDTGGTTG
                     SGARPGATGGMASGGRAPREPAGTSVVTFVHGIVDANDILVCFAAGSGDERRYLGDPA
                     PKGGLSYGMTWVLPEQVVAAESETLQPVVLTGELELVAGLDCERAVARALEEQRTADE
                     VKGEGGAGAQAGAGGSDGGQRGSGDAGATGHALPSGGAAAEGAGGAGAGQANVLELVL
                     EGGAGGAGGAEGVTGASTPRLRVAALPAVPREEADSGQSQLFVATGCMGSPAYAGPLA
                     ERACGDAYTPRNPTVSGVWVTLSRAASPVRLGLQVVHASRANGTVQVWLASDDPSKPW
                     LRAAHAASYGTIEPRPANTSYSAAEYGIEDASYHLQVQQDSAVLFEDTWEDVLTRVDA
                     ERPLNGQNYTLVLIGPDFTLRSRGFWNPPRVTWVVNDPGL"
     gene            complement(4816..>5412)
                     /locus_tag="VFQ61_33750"
     CDS             complement(4816..>5412)
                     /locus_tag="VFQ61_33750"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET9959521.1"
                     /translation="KHVIVAVASELESRAMSIPVVVDPVMVAKGGASLLREDARTALI
                     ERLIPLCALLTPNLPEAAALTGLPAEDEADMDRLADALLELGAAAVLVKGGHLASDRI
                     VDLLRTADGLEHRFESKRIVTRSSHGTGCTLASAIATGIAEGMTLVSAIDRARTFVRR
                     ALEFAPGLGAGHGPLHHGWPITHPEAQRTPAKQVFQGE"
BASE COUNT          959 a         1902 c         1652 g          899 t
ORIGIN      
        1 tcaaaccagc ccgcgcacgc tcacgctcga cgtgggcgtg acgtgcgaga gtgagctcgt
       61 gccgcgcacg cctacgcgag cattggcttt gctcgatccg ctcgcgacga gcccactgcg
      121 gtttggcgtg cgcacggccg cacctgcccc gggcgagatg ctgtccttcg acctatccgc
      181 tcgagacgaa gacagcgatg gccgagagga ttttcgtctg aacgtgagct tggccgtagg
      241 cagcaccaca cccctcgcga gcgcgccgct cgttttcttg gatcgcgccg ccggcgtttc
      301 ccgcgaagca cgcgaacctg cgcgggccat caacgaagcc ctgaaagcag agtccgtgcg
      361 cgcgcaaaga aagaagacgg ccgaagcggt gttggcccga gtggatgcca cccgccggct
      421 gctcgcgacc ctgtgcgccg aaggtgcaac ccccagggtg ttcgactgga acggggacaa
      481 gttcacctgc ggttcgttgc aggacatcgt cgatcgtctc ggtacgctcg aagtcaccgc
      541 gcatagcgcg ctcagcgact gggaatcggc cttgggggct ttggcgagag atggctggta
      601 cttcggtaag atgcgcgcga ccgcgcgtag cgcgttgatt aaagagctgc agaagcatct
      661 cacgcgcgtt cccgtcaccc gggtttctct cagtacggtc cccaaagcca cgacgccgcc
      721 gagcctcagc ccgctgagct tcgatagcgg cggggcgctc tggctcgaga cggacggcgg
      781 gctcgtgcgg atcgcgccga gcgacacccg tgaggagccg gtcgcaccgg atccaagcgt
      841 cagcggttgg cccgtagagc cagtcgggcc gcgcgacgag cgctggatct cggttagtta
      901 ttcttgcgat cgatccgagg tcagcttgct gctcgcgggc gcgagtgatc ccactcccgc
      961 catcagcggc ctactcgcac cccgcccggg tgtatgcgcg ggaggtcgtt tcgatgaggc
     1021 gttcgcacca gcattcattg cggcgcacga tgcacgcgag gtgttcgttg gaggccagcg
     1081 cttcggcgac aagaagctcg gcgcccccgg accacgtggt tccgctcggt caccgagcgg
     1141 tgcacgcgtc gcgatcccga gttcgctagg gctctggatt caagccgaga agagcgagct
     1201 ctggagcgtg gacaactggg acacgcccgc ggcaagaaac tgcgtcgtcg atgacaacgc
     1261 gcggcgagcc gcatgcatcc gcggcacgcg cgccgaactc taccttcgga acgacagcac
     1321 gcagccgtag cgcactccgg agcgcgcgta cctcttcgag gggcgcgctc ggtgtgctgt
     1381 tcttcctcgg ctgggtttgg ccgggttctt ggcacggcgt acacgggcga gttgcgccgc
     1441 aattgccgcg caactcgggt acgaccgaaa ctcgggcccg ggagagcagc gcgctctccc
     1501 cgaggcgtcc gcgaatcagc tgccgctcgg cgctttggcg cgctcgagat agctgcgcga
     1561 gcgcgtgtcg accttgatcc aatcgccctg gttcacgaac aggggcactg gcaccgaagc
     1621 gcccgtcgag atttttgcgg gcttcgtcac gttcgtggcc gtgtcgccac gcacgccggg
     1681 ctccgtctcg gtcacctgca ccacgatgtg cggcggcagc gtgacgctga ccgggttgcc
     1741 cttgtagatg atgatcgaca cttcgatccc atcgatcaaa aagccggcat cgtcgcccac
     1801 ggcttcgcgc tgcaccgtca cctgctcgcc ggactgcggg ttcatgaaaa cgaacgcatc
     1861 gccttccggg tagatgtact gcagcgagcg ctcttcgacg tctgccgcct cgagcttttc
     1921 acccgaacgg atgttgcgtt cgagggtgtt cccgttcagc aggttcttca tgcgcgtgcg
     1981 ggtgaaggcc tgccctttgc ccggcttcac gaactggaag tcgacgacga tgtacggctg
     2041 cccgtccatc atgagcttca aacctttgcg aatatcgctc gtatccatgt gcttcctcga
     2101 gtcacccggc cgtcagccgc gcacggggcg gcccgggtgc tacccgagcc ggccctgcct
     2161 ctagcgaggg atcgctccgc gttcaacccc gaaaaccagg cctgccccaa aagtccgcac
     2221 attcaggcgg gtcctcctcc cgaggcgctt cgcgctccac gcggggcccc ggccccggtt
     2281 gcgacgtctg ctcggccccc gccaagagca aatccgggcg gttcccctcc cgagacgctt
     2341 cgcgttgcgc acgggggccc agccccgctc gtgacgtctt gctcggaccg tcggaaggct
     2401 gtagggagca gcgactgccg gtggccgtcc gaggttggct ttgtttccgc catgcgggca
     2461 gccacgcgag cggcagccga cccgcccccc gcacgagaca ctagcctggc gccgaagaca
     2521 tggaggaata cgagctcgag gagattaaac gcgaaatcgt ggagagccgt tcgctctcga
     2581 tcaagacgaa caacttgatc aacgcgctgg ccgccgacct caagagcatc gcgaaacgcc
     2641 agcaaagcta cgaacgccgc gtcttcgtga acagcgcgac ggcgtacgcg gtcacgatcg
     2701 cggtcatcct cgtgttcgtc aagctcgcct gggacatccg gctcgaggcc gtgcgcggcg
     2761 aatcgcgcga gacgcgcgac cgcgtcgagc agctcgagaa ggaactcaag aatctccagg
     2821 gccgcgagga agcgctggcg cgggtgagtc ggcgcgcctc ggagttctac caactcatca
     2881 cgttgaataa gcgcaaggag ctaatcgaag gtttcacgga aatcgcgaaa ctcgaactct
     2941 cccccaccga gcgctcggta ttcgaggcgg cggtcgagcg cgcccgcaac gagctatccc
     3001 tgattgccta ccagaacggc ctcgatcact cgcgcatggg ccgcttccac gaagcccagc
     3061 agtcgttccg cgaatcgctc aagtacaaga cggacgccgc gcatagcccg caagcgaatc
     3121 ttcaattgtc ccgggcgttg atcaagctgg gcatgcatcg tgacgccatc ccaatcttga
     3181 tgcagctatc cgaagcctcg cccgacaaag aagtcatgga cgaagccacg ctgtctttgg
     3241 cttttgccca gctcgacatc caagcctgga acgacgccaa gaacacgctg cgcgccttca
     3301 ttcgacgctt ccccagcagc gtccacatca acgacgccaa aggcaagctg gccgaagttc
     3361 agctctatca ctgagcttta ccactgaagt ccgaatacca aggacgacta aaggcctgga
     3421 tcgttgacca cccaggtcac gcgcggggga ttccaaaacc cgcgactgcg cagggtgaag
     3481 tctggcccga tgagcaccaa cgtatagttc tgcccattga gcgggcgctc ggcgtcgacg
     3541 cgcgtcagca cgtcctccca agtgtcctcg aacaacaccg ccgagtcctg ctgcacttgg
     3601 aggtgatacg aggcgtcctc gatgccatat tcagccgcgg aatagctcgt gttggcgggc
     3661 cgcggctcga tcgttccgta gctcgccgcg tgggcggctc gcaaccaggg cttgctcgga
     3721 tcatccgaag caagccagac ttgaacggta ccgttcgccc gtgaggcgtg cacgacctgt
     3781 agccccaaac gaaccggcga ggcggcacga gacaaggtga cccacacgcc ggataccgtg
     3841 ggatttctcg gcgtgtatgc gtcaccgcag gcacgctcag ccaagggccc cgcgtaggct
     3901 gggcttccca tgcaacccgt ggcaacgaac agctggctct gcccggagtc ggcctcttcg
     3961 cgcggaaccg ccggcagagc cgcgacacgc aatctgggag tagaggcgcc cgtcacgcct
     4021 tcggcgccgc cagcaccgcc cgcccctccc tccagcacca actcgagtac gttcgcctgg
     4081 cccgcacccg caccgccggc tccttccgcg gctgcaccgc cggaagggag cgcgtggccc
     4141 gttgctcccg cgtcaccgct accgcgctgg ccgccgtcac tcccgccggc tccagcctgc
     4201 gcccccgcac caccttcgcc cttcacctcg tccgcggtgc gttgttcttc gagcgcacga
     4261 gccacggctc gctcgcagtc cagccccgcg accagctcga gctcaccggt caatacgacg
     4321 ggctgcaggg tctccgattc cgcggccacc acctgctcag gcagcaccca agtcatcccg
     4381 taggagaggc cgcccttcgg cgccgggtcg ccgagatagc gacgctcatc cccgctccca
     4441 gccgcaaaac ataccaaaat atcgttcgca tccacgatgc cgtgcacgaa ggtgaccaca
     4501 ctcgtcccgg caggttcgcg cggcgcacgc ccgccggacg ccattccccc ggtagcacca
     4561 ggtctcgcgc cactgcccgt cgttccgccc gtgtccatgt tgccgccgcg cgattctacc
     4621 ccgcccgatg cagccgccga ttgcacgggt tcactgccgc cgcacgcaac gggccccacg
     4681 ctcatcagcg ccccgaccag gctcacgcct ccaatgggga ttgctcgccg cgcgagatgc
     4741 cccttaacgc catgagcccc tgttcgacgc cgatgcatgc tcaactgatc tgcctttcca
     4801 atcgttcgaa cgccactact cgccctgaaa aacctgcttt gctggcgtgc gctgcgcctc
     4861 cggatgcgta attggccacc cgtgatgtaa tggtccgtgc cccgccccga ggcccggcgc
     4921 gaactcgagc gcgcgtcgta cgaaagtacg cgcccgatcg atcgcggaga ccaaggtcat
     4981 gccttctgca atgccggtcg cgatcgcgga cgccaatgta cagcccgtgc cgtggctgga
     5041 gcgagtcacg atgcgcttgg actcgaagcg atgctccaaa ccgtcggcag tgcgcagcag
     5101 atccacgatg cgatcggacg ccaagtgacc gccctttacg agcaccgcgg ccgcaccgag
     5161 ctccagtagc gcgtccgcca accgatccat atccgcctcg tcttcggcgg gcagccccgt
     5221 cagcgcagcg gcctccggca agttgggggt caacagggca cagagcggga tcagacgttc
     5281 aatcagcgcc gtgcgcgcgt cctcacgcaa caacgaggcg ccgcccttgg ccaccatcac
     5341 cggatcgacc acgacgggga tgctcatcgc gcgcgactcg agctcgctcg ccaccgctac
     5401 gatcacgtgc tt
//
LOCUS       DASTKU010000952         7530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1975134, whole genome shotgun sequence.
ACCESSION   DASTKU010000952 DASTKU010000000
VERSION     DASTKU010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1975134"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1362
                     /locus_tag="VFQ61_33755"
     CDS             256..1362
                     /locus_tag="VFQ61_33755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959522.1"
                     /translation="MKSRTLFAVLTIAVGAQTAGCSADASSEQDLASDQSELFASSSK
                     LWRAPRHFQTTKISVCVTNPAGYSKYVGWIRDILANTWSAAAKVKFEGFGACGSADVR
                     LTFADGTPHVLALGTDATEVWLKPWTAEDESLEFLARYLVEKNAIHEFGHILGLAHEA
                     NHPDSTCDDTQGPNGDVYWEYDEDSVMNYCAPIRNYLSDLDKEGIAKLYGGAGTRSGK
                     KYGLWNEGNGKFFYLFSDSHGDRMQFSGGLNTFIIERDAGSGSIAYGDKIGLKRGNKY
                     ISATANAMNNTGSLWSTTSTFRQWETEHTAEGNGGEHIDVRDPFRIYLPVGNKKFYLS
                     TRKDQHSSRYLDEIVVVTNPDQGAEWRLMGDFNE"
     gene            complement(1432..2883)
                     /locus_tag="VFQ61_33760"
     CDS             complement(1432..2883)
                     /locus_tag="VFQ61_33760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HET9959523.1"
                     /translation="MAFSRLARLPLTTFALTCGLWGCSQSSGGAAQSPNTGGTSTGGT
                     STSSSGGSVVSGGAGTTAGTGNSGANPGSGGLQGSGGAVGGAGGTSANTGGSPSSGGA
                     AGGAVATGGGSGKGSGGSAGSPVGGTAGSVGAGGSAGKGGESGATGGVGNSGGSAGGG
                     GAAGGSTSSEGATLVPDPSWTCGMADGIPAPTTGTPAFSVTLNVSATHDVGNTQFGKR
                     RQLDVSGGTITGDKLKGTVQTGGLDYELTLSTGAMELEEVLIWKTSDNVSMFVRVCGV
                     AAPGDSVVRIVPDIEAPTSGSASWLNTAKLVGTRKVDPGGSKITLDVYDVSTAPAGAP
                     KIQLKDPAGVPNNPWDCITGNGSKGSEVLTENVTLGSSFSISNAKRGSRNVIPITGGT
                     TTGKVVGKILNGGADYQLAGSSGTTLDARYTLAPNDGNFIIVRNCGPINALIPLFEAA
                     SSGPYAFLTTGKFLSSQPGAGAGGVSITFYERK"
     gene            complement(3134..5002)
                     /locus_tag="VFQ61_33765"
     CDS             complement(3134..5002)
                     /locus_tag="VFQ61_33765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959524.1"
                     /translation="MPLSPSLPLVAYAEALCNSAHVLFVGDARSGVPEALVERGARWV
                     HVCDPDSARQSEAAARSQHKAVTFGRLDEGPLSLRDGFFDLVLLEDLSRAPDPAAVLT
                     PLARLLAPRGVALIGAPNRSVTEPWLLTSSEGEASSVDYYRLYELVSSTWPAVRMLGE
                     VPFVGYAIVDFSAEDEPSPVLDTSLLGGSAEEPEYFIALAGRERRALDEYMVVQVRAA
                     ELRTAEVPSTEPRSTELRSTDRASVTPPAAVPAVSPELARSPAQSAGGKSAAPQLTPR
                     PPVAQPAAALSSADLERLARVSELERRVAQQESWIQQVEAQAAAADERADAAETELDS
                     LKERLGNLTQNHGQERTALVRERDALKRDVERLSRRANDQEDLLVAKQAELTTKQEEL
                     KAKQAELNAKQAAFDAKQAELTSKQSELTTKQAAQQAEQGETANLRSKLSALEAELQQ
                     LRAQYADSQRTVNALQQTATQSQAQRTQAEQNAVARQAELERAREELEQVRGELEQTR
                     AEQQRAAGELADLRDAVARQETQLREALTAQEQEIQAELARLERQLAERGENVRRLER
                     ELRETERVGRELVRKMTQPEGPERARAMAARLAEAEAELVTLRWSLEQLKPSATAS"
     gene            complement(5155..5745)
                     /locus_tag="VFQ61_33770"
     CDS             complement(5155..5745)
                     /locus_tag="VFQ61_33770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959525.1"
                     /translation="MSLVDENAFGLLVAFHDLEDAEYSLEPPEFVVRFTEFTSEVLSY
                     AEETPLGPRTNLLELGHALYFELADGDQSTDLIAWLRNLRTRLVERGFEVCAVLSHGS
                     RWVEEADAGSSEPWPRLEELGGTYRWMRASRPSEPLRRALAAEVATHGGLGEDSDGWG
                     SGCYLDSEAMEALGKTLKNAPTPLEVAGATFFRIGR"
     gene            complement(5755..6381)
                     /locus_tag="VFQ61_33775"
     CDS             complement(5755..6381)
                     /locus_tag="VFQ61_33775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HET9959526.1"
                     /translation="MLSGLGAALRARVRALSASTGSLAAITPLLPHWLEARLPLTVRH
                     RLAEILSPGLLTGLAIFSVVSFLGSLVGVPYFFARLPADYFSRREQAALGIDPGPRPW
                     YQTTLRVLRNVLGWSLILLGIPLFVLPGQGLLTVLVGILMVDFPGKHRLERAIISRPR
                     VLATINALRKRTGRPPIETRLSWLPPDPTSPETSVADTKKIGEPPHLG"
     gene            6760..7290
                     /locus_tag="VFQ61_33780"
     CDS             6760..7290
                     /locus_tag="VFQ61_33780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959527.1"
                     /translation="MAHCLLNRLRLGWIALNGSFMGVSCIGMSMAVVASLSGCRETPG
                     VKAQDTEGRRFSIVCEGESACTVRVESGDPAFLRLASNGRLVGVCEARSSHPADCRAL
                     VCTHDGECPTQRGVSGTCVGGLCVDSTHDLATDDAAMLCLWGTGLGHESREQIERYAL
                     AANCGSPCVIPKPCER"
     gene            complement(7347..>7530)
                     /locus_tag="VFQ61_33785"
     CDS             complement(7347..>7530)
                     /locus_tag="VFQ61_33785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959528.1"
                     /translation="DLNQHSILAFCFNTYFLGLFSDRFKTHQIGRAPYDAPGSELDLR
                     TAEVAAVEQGVNASMA"
BASE COUNT         1285 a         2343 c         2462 g         1440 t
ORIGIN      
        1 caggggcgcc tgacatcgcg agcgcgagcg ggttgcccga gttgtccgag ttgcccgccc
       61 cgagttgttt gcccggtcag gcgcgcagag acgaaccatc gagcttgagt gataggtgtt
      121 gtggatgcga gttcgttgga actagcgata agacgccgag gtacggttgc cgctcggcgt
      181 atcacgcgcc tggtacacga cttgctgaac ccgactgggc gcgacacgac gccggctcaa
      241 gcgggaaaac cagatatgaa gtcgagaacg ttattcgcag ttttgacgat agccgtaggt
      301 gctcagacgg caggctgctc ggcggacgcg tcgagtgagc aggacctcgc cagtgatcag
      361 tccgagttgt ttgcctcttc gagcaagctg tggcgggccc cgcgacactt ccaaaccacc
      421 aagatctcgg tgtgcgtcac caaccccgcc gggtacagta aatacgtcgg ttggattcgc
      481 gatattcttg cgaatacgtg gtcggccgcc gcaaaggtca agttcgaagg cttcggcgcg
      541 tgcggcagcg ccgacgtacg tctcacgttc gcagacggga ccccccacgt gcttgctcta
      601 ggaacggacg ccaccgaggt ctggctcaag ccgtggactg ccgaggacga atccttggag
      661 ttcctagcgc gttatctggt ggagaagaat gcgattcacg aattcggcca tattctgggc
      721 ctcgcgcacg aagcgaatca tcctgactcg acctgtgatg atacccaagg gcccaatggc
      781 gacgtgtact gggaatatga cgaggactcg gtcatgaact actgcgcgcc cattcggaac
      841 tacctcagcg acctcgacaa ggagggcatt gccaagctct acggcggcgc gggcacccgc
      901 tccggcaaga agtatggcct ctggaacgaa ggtaacggca agttcttcta cctgttctcg
      961 gactcccatg gagaccgcat gcagttctcc ggtggcctca atacgttcat catcgagcga
     1021 gacgctggca gtgggagcat cgcctacggc gacaagattg gcctgaagag ggggaacaag
     1081 tacatctccg cgacggccaa tgcgatgaac aacacgggct ccctttggtc caccaccagc
     1141 accttccggc agtgggagac ggagcacacc gccgagggca acggcggcga acacatcgac
     1201 gtgcgcgatc cgttccggat ttatctgccc gtcggaaata agaagttcta tctatccacc
     1261 cgcaaggacc aacattccag caggtatctc gacgagatcg tggtcgtcac caatccggac
     1321 caaggtgccg agtggcgcct catgggtgac ttcaacgaat agcgttatcg ccgagcttcg
     1381 tttgcagcga gtgcgtaggg cggcctggct ccaggtcggg atgttgggcc gttacttgcg
     1441 ttcgtagaag gtgatgctca cgccgccggc accggcgccc ggttgcgagc tgaggaattt
     1501 gccggtggtg agaaacgcat acgggccgct cgaggccgcc tcgaacagtg ggattagcgc
     1561 gttgatgggg ccgcagttcc gcacgatgat gaagttgccg tcgtttggcg ccagggtgta
     1621 tcgcgcatcc aacgtggtgc cggatgagcc agcgagttga taatccgcgc ccccgttgag
     1681 gatcttgccg accaccttgc cggtagtggt gccccctgta atcgggatga cgttgcgact
     1741 gccacgcttg gcgttgctga tggagaacga ggagcccagt gtgacgttct cggtcaacac
     1801 ttcgctgccc ttgcttccgt tccccgtgat gcagtcccac gggttgttgg ggacgcccgc
     1861 aggatctttc agttggatct tgggggcccc cgcgggtgct gtcgatacgt cgtagacgtc
     1921 cagcgtgatc ttgctgccgc ccgggtcgac tttgcgtgtg ccgacgagct tcgccgtgtt
     1981 gagccacgag gcagagccgg aggtgggggc ttcgatatcg gggacgatgc ggacgaccga
     2041 atcaccgggc gcagccacgc cgcatacccg cacgaacatg gatacgttgt cgctcgtctt
     2101 ccaaatcagc acttcttcca gttccatcgc gcccgtcgag agcgtcaact cgtagtcgag
     2161 gccgcccgtc tgcacggtgc ccttgagctt gtcgcccgtg atcgtcccgc ccgagacatc
     2221 gagctgacgt cgctttccaa actgggtatt accgacgtcg tgtgtcgcgc tgacgttgag
     2281 ggtcacgcta aatgcgggag tgccggtggt aggagcagga atgccatcgg ccatgccgca
     2341 ggtccacgac ggatcgggaa cgagggtcgc tccttccgag ctcgtgctgc cgcccgcggc
     2401 gccgccaccg ccagcgctgc cgcccgagtt tccaacaccg ccggtcgctc cagactctcc
     2461 gcccttgccc gcggagcctc ccgccccgac cgagcccgcg gttccaccga ctggagagcc
     2521 ggcgcttccg ccggagcctt tcccgcttcc accacccgtc gccacggcac ccccggcggc
     2581 tcctccagag cttgggcttc ctcccgtgtt tgcggatgtt ccgccagcgc ctccgactgc
     2641 gccaccactg ccctgtagtc caccgctgcc ggggtttgcg ccggagtttc cggttccggc
     2701 agttgtcccg gcgccgcccg agacgacact gccgcccgaa ctactggtgc tggtgccgcc
     2761 cgtgctggtg ccaccggtat tcggggattg agcagcaccc ccgctcgatt ggctacagcc
     2821 ccagaggccg cacgtcaggg cgaacgttgt gagtggcaat ctcgctagac ggctgaaagc
     2881 catttccaat cttctccaca aagagccatt gagcagccga agcgagagca gcgtcgccca
     2941 cgcccgcgtc acacgcgaga tgaaatcaca tgcgagactc cgctcgctac ggtcgatttg
     3001 tgccctcccc acgcccctgg aaaattgcgc atcagcgcca cgcatgcgtc ctcgcccgac
     3061 ggatagccgt cggcgagttg cagctgcgat ttggggccca gcgcgcaaag ctccgtacgt
     3121 cgggcgcctc cgcttacgaa gcggtagcgc tcggcttcag ctgctccaag gaccagcgca
     3181 acgtgacgag ctccgcttcc gcttcagcga gtcgcgcggc catggcgcgg gctcgctctg
     3241 gcccttctgg ttgcgtcatc ttgcgcacca gttcgcgccc gacgcgctcg gtttcgcgca
     3301 gttcacgctc gagccttcgg acgttttcgc cccgctccgc gagttggcgt tcgagccgtg
     3361 caagctccgc ttggatctct tgctcttgag cggtgagagc ttcgcgcagc tgagtttctt
     3421 gccgggcgac ggcgtcgcgc aggtcggcga gctccccggc cgctcgttgc tgctcggcgc
     3481 gtgtctgttc gagttcgccg cgaacctgct cgagttcctc gcgtgctcgt tcgagttcgg
     3541 cttgccgcgc aactgcgttt tgttcggctt gagtgcgctg ggcttgggac tgcgtggccg
     3601 tctgttgcag ggcgtttacc gtacgctgac tgtcggcgta ctgcgcgcgg agctgctgga
     3661 gctcggcctc gagcgcgctg agtttgctgc ggaggttggc cgtttcgcct tgttctgcct
     3721 gctgcgcggc ttgtttggtg gtgagctcgc tttgcttgct cgtgagctct gcttgctttg
     3781 cgtcgaacgc tgcttgcttc gcgttcagct ccgcttgttt ggccttgagc tcttcttgtt
     3841 tggtggtgag ctcggcctgc ttggcaacga gcaggtcttc ctggtcgttt gctcgtcgtg
     3901 acagtcgttc gacatcgcgc ttgagtgcat cgcgctctcg aaccaacgcg gtgcgctctt
     3961 ggccatgatt ctgagttaaa tttccgagcc gctctttcaa tgaatcgagc tcggtttctg
     4021 cggcgtccgc gcgctcgtcg gccgcggccg cttgcgcttc gacttgctgg atccagctct
     4081 cttgctgggc aacgcgacgc tccagctcgc taacccgtgc cagtcgttcg agatcggcac
     4141 tcgacagcgc tgctgcaggt tgcgccacgg gcgggcgcgg ggtgagctgt ggtgcggcgg
     4201 actttccgcc tgcgctttgt gcggggctcc gggcgagctc gggggagacc gccggaacag
     4261 cagccggtgg cgtgacgctc gcccgatctg tacttcgaag ctctgtactt cgtggctctg
     4321 tacttggaac ctctgccgtc cgaagctcgg ccgcgcgaac ctgcacgacc atgtactcgt
     4381 cgagcgcccg ccgttcgcgg cctgcgaggg cgatgaagta ctcgggctcc tcggcgctac
     4441 ccccgagcag ggaggtatcg agtacggggc tgggctcgtc ctccgcggaa aagtcgacga
     4501 tggcgtagcc gacgaacggc acttcaccga gcatccgcac ggcgggccag gtgctgctca
     4561 ccagctcata aagccgataa taatctaccg agctcgcctc gccttcactc gacgtcagga
     4621 gccatggctc cgtcacggag cgattcggcg ccccaatcaa agctacacca cgcggcgcca
     4681 ggagccttgc gagtggggtt aggaccgcgg ccgggtccgg tgctcgcgag agatcttcga
     4741 gcagtacgag atcgaagaag ccatccctga gcgagagtgg tccttcatcc aaacgtccga
     4801 atgtgacggc cttgtgctga ctgcgcgcgg cggcttcact ctgccgtgca gagtcggggt
     4861 cacagacgtg cacccagcgc gctccgcgct cgaccaatgc ttccgggacg ccgctccgag
     4921 catctcccac gaacaggaca tgagccgaat tgcagagtgc ttcggcgtac gcgaccaagg
     4981 gtagtgaagg cgataagggc atccgcgggg gcgcaaggct agcatcgacc gcccaaagcg
     5041 cggctcaaga aatgggctgc gatccaaacc ggccgcggcg ccgcgccagc ggtcatgcgc
     5101 ggctgccctc ccgaacggcg gcgctcctgc gctcccaaat cggccgtaga aggctcaacg
     5161 cccgatgcga aagaacgttg ctccagccac ttcgagcggg gtgggcgcgt tcttgagggt
     5221 tttgccgagc gcctccatgg cttcactatc caagtagcag ccgctgcccc aaccgtcgct
     5281 atcttcgcca aggccgccgt gggtggccac ctcggctgcg agtgcgcggc gcaagggttc
     5341 gctcggacgc gaggcgcgca tccagcgata ggtcccgccc aactcctcta gtcgtggcca
     5401 aggctcactg gaaccggcat ccgcctcttc gacccagcgg ctaccgtggc tgagcaccgc
     5461 gcagacctcg aagcctcgct ccaccaagcg ggtccgtagg ttgcgcagcc acgcgatgag
     5521 gtccgtgctt tgatcgccgt ccgccaactc gaagtacaaa gcgtgcccga gctcgagcag
     5581 gttcgttcgg gggccgagcg gtgtttcctc cgcgtaagac aagacctcgc tcgtgaactc
     5641 cgtaaaacgc accacgaact ccggcggctc caaagagtat tcggcatcct ccaggtcgtg
     5701 gaatgcaacc aatagcccga acgcgttctc gtcaaccagc gacacgcccc ggttttaacc
     5761 tagatgtggc ggctcgccga tcttcttcgt atccgccacg ctggtttcgg gcgacgttgg
     5821 gtcgggagga agccaagaga gccgggtctc gatgggcggg cgaccggtgc gtttacggag
     5881 cgcgttaatc gtggcgagga cgcgcgggcg cgaaatgatg gcgcgctcca gccgatgttt
     5941 cccgggaaaa tcgaccatca atatgcccac caacaccgtg agcaagcctt gcccgggcag
     6001 cacgaatagc ggaatcccga gcaggatcaa cgaccacccg agcacgtttc taagcactct
     6061 gagcgtagtt tggtaccagg ggcgtgggcc aggatcgatg ccgagggcgg cttgctcgcg
     6121 acgcgagaag taatcagccg gcaaacgcgc gaagaagtac ggaaccccaa ctaggctgcc
     6181 gagaaaactc acgacgctga agatagcgag cccggtgagc aaacctggcg acaggatttc
     6241 cgcgagtcgg tgccgaaccg tgagcggcag ccgcgcttcg agccagtggg ggagcaatgg
     6301 cgtgatcgcg gcgagtgaac cggtgctggc gctcagtgcg cgaactcgag ctcgtagggc
     6361 ggctcctaaa cccgagagca tgcccaatga tacgagcaac cggggtcgcg cgtgagggct
     6421 tgctccaact tcccgtgcgg agctgaaaac gtaacggcgc gcgatttgcc ccggcacgac
     6481 cgcggtttgg atgccgcggc cgttattggg ggggccggcg cggtgttgcg ggggaggagc
     6541 gaggagcggg ctgcgcgtgc gggtgaactg gacgggcggc gcgctcgtta cagggtggcc
     6601 acggaacaga cggccgtgcc tgattggatt gcgcaaagac cagggttgct tgcgcaggtt
     6661 gcttgagcag gttgcttgag caagggccgt cgctcgatcg aggttcgcgc aacccgccct
     6721 tcaggaaggc gggccgtggc cgttggatcg caaagacccg tggcgcattg tctcctgaat
     6781 cgcttgcggt tgggttggat agccctgaat ggatctttca tgggcgtgtc ctgcattgga
     6841 atgtccatgg cggtggtcgc aagcctctcg ggctgtcggg agacccccgg ggtgaaggct
     6901 caggacacgg agggccgtcg cttcagcatc gtgtgtgagg gtgagtcggc gtgcaccgtg
     6961 cgcgtggagt cgggtgaccc tgcgttctta cgcttggcat ccaacggacg cttagtcggt
     7021 gtgtgcgaag cccgcagcag tcaccccgcc gattgccggg ctttggtctg cacgcatgac
     7081 ggggaatgcc cgacccaacg cggcgtgagc gggacttgcg tggggggtct ttgtgtggac
     7141 tcgacgcacg atctcgcgac cgacgatgcg gcgatgttgt gtttgtgggg gacgggccta
     7201 ggccacgaga gtcgcgagca aatcgagcgt tatgcgctgg ccgccaactg tggctcgccc
     7261 tgcgtgatcc cgaaaccctg tgagcgttga cggggcagag cgagcctctc gataggatgg
     7321 cacagacggc cgagcgcgat tgcggctcac gccatgctgg cgttgacgcc ctgctcgacg
     7381 gcggcgactt ccgcagtgcg gagatcgagc tcggagcccg gagcgtcgta cggagcgcgt
     7441 ccgatctgat gggtcttgaa gcgatccgag aatagaccga ggaagtaggt gttgaagcag
     7501 aacgccagga tcgagtgctg gttgagatcc
//
LOCUS       DASTKU010000953         9241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1079931, whole genome shotgun sequence.
ACCESSION   DASTKU010000953 DASTKU010000000
VERSION     DASTKU010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079931"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFQ61_33790"
     CDS             complement(<1..620)
                     /locus_tag="VFQ61_33790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9959529.1"
                     /translation="MEAQVDRRPYAEGAEQKAVLTGLLVGVLVVGIDSTIVATILPRM
                     LSEFGDLPLAPWVFASYMLMQISSAPILGKLSDRFGRKRVLLFGLFTFLLGSELIAFS
                     RSFGELILFRGLQGIGAGAILPAALATLFLDFPPEMRPRLQGNFSAVYGVSNILGPLL
                     GAVLAEHVGWRYAFHINIVFVSISAYCISRSLRDTRGSHRKGQLDWA"
     gene            641..823
                     /locus_tag="VFQ61_33795"
     CDS             641..823
                     /locus_tag="VFQ61_33795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959530.1"
                     /translation="MQNKTQECIDFMIHARLGIGLPSLVFQVARGCRKQGRTQTHLRR
                     KDRCAADLAAPQLVWM"
     gene            complement(1147..1923)
                     /locus_tag="VFQ61_33800"
     CDS             complement(1147..1923)
                     /locus_tag="VFQ61_33800"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET9959531.1"
                     /translation="MERLRIRDTGIRVALPRGAARVGSSSVLLLHGAGASHRQWGPFI
                     EALAPGFVPIAVDLPGHGESDGTVAPSLDAAVEFLAELFEALRLPTPLWCIGHSAGGV
                     LALSLALHYPKRLSGLCTIASAPRLQLHPDFVQQARHGRFEYELARGGFARSDDPWAE
                     LVYDDLCKVRFGPASMFDGEHLDVRAALPNLRVPLLAIGAGSDRIVSPRHSRSLAAGV
                     PRGTCVTLNDAGHYVHLEQPRQAAREFHDWAERLQREQGA"
     gene            complement(1928..3256)
                     /locus_tag="VFQ61_33805"
     CDS             complement(1928..3256)
                     /locus_tag="VFQ61_33805"
                     /EC_number="4.2.99.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043904 - isochorismate pyruvate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="salicylate synthase"
                     /protein_id="HET9959532.1"
                     /translation="MSAERYVFEFRHSVDPTALVEHLKVQGLVRDYLMYRNHTGEGET
                     IVALNSRARVRVSRDVVELDGDQPEKHSRVRAEDPLKQVERALADVPLRNWTAYGYIG
                     FDLARFYFDYRKDTGYPSLFFMIPETELRIARDSVRVTTALPAQVWKDVLDAARKATR
                     HPQSATPPQPGSSDHQEYVARVQTLIGWIQEGKLQKAILSRRVEVPGELDVLGTYLNA
                     RDANNAARSFCFELSGVRGVGFSPELFLEATSDGQVITNPLAGTRPRGGTLDEDQRVR
                     AELVADSKEVKEHAMSVRLAQEEMSRVCHAETVRIYDFMDVKQFRCVQHLSSRVSGQL
                     KAGFTTWDAAKILFPAITVSGVDKAAALKAIGQLEEGPRGVYGGAVGWIDQSGAADLC
                     LAIRSVYGYDQRTVLNAGAGIVAESRPEMEYMESVNKMKTMATHVTLASS"
     gene            complement(3246..3725)
                     /locus_tag="VFQ61_33810"
     CDS             complement(3246..3725)
                     /locus_tag="VFQ61_33810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9959533.1"
                     /translation="MAGHTENTIVVLRDYETVLDVSNRVELWPVLFTEYSKAEVLERV
                     GDEILFRLTTHPEDGRPARTWTSRRKVDRAAGVATAERLDPTFPFAYMKIRWVYERLP
                     KEVGVVMTWIQDFEVHADCPWSEERMESFLNRNTRVQMASVKRGLESWSGKEQSHVG"
     gene            complement(3739..4575)
                     /locus_tag="VFQ61_33815"
     CDS             complement(3739..4575)
                     /locus_tag="VFQ61_33815"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET9959534.1"
                     /translation="MAQLVDLSVLVENSPSEPMRIEVQRLDPVRGARHFCFEAFWNRR
                     LPLPKRVQQALRFVSGKARILPKDFPGRAFLTLDTVKLPTHMGTHVDAPVHYGPTSDG
                     TPARSVDQLPLEWFFGRGVRLDLRHKGKAQYIQPEDLEHALQASGHELRRGDIVLIWT
                     GTDRLWGKREYFSDAPGMSRAATEWLVERGIRVIGIDTYGFDRPFGAMLGDFYRTRDP
                     RHLWPAHFYGREREYIQIERLANLDKLPPTGFDIACFPLKIRGLDASWVRAVGILPEA
                     NN"
     gene            complement(4559..4882)
                     /locus_tag="VFQ61_33820"
     CDS             complement(4559..4882)
                     /locus_tag="VFQ61_33820"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET9959535.1"
                     /translation="MQHLIENAAPVAESFFALTKSIRDASPLGPKLNELVILGVFVAH
                     GGTRGIQTHVERAQEHGATREEIVAAITLALPVVGISRVNEALEVAIRTIQSKEGRSR
                     DGTAR"
     gene            complement(4885..5274)
                     /locus_tag="VFQ61_33825"
     CDS             complement(4885..5274)
                     /locus_tag="VFQ61_33825"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9959536.1"
                     /translation="MADSKLCISEREVTERTDRGGTYRVMITPGSVNAKQLILGKATV
                     PPGERVKAHVHDYSEEAFFVVQGTGCAILDGIGTVEFRAGDAVLVPKGVTHSIENRGS
                     EEVVVVFASAPLAPRPESGHRNLEKGS"
     gene            complement(5267..6868)
                     /locus_tag="VFQ61_33830"
     CDS             complement(5267..6868)
                     /locus_tag="VFQ61_33830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HET9959537.1"
                     /translation="MTSLVQDFKSGVSLNANSATRGVSDSIAATLPRSFNVAAALLQR
                     NRERAAARALICGDRSFTHGQLAEAVARFAARLLAAGVRQEDRVALLLPDGPELVIGI
                     LAAIWMGAVAVPINTAYSSETIEFILEESRARALLIDEPGSARLSASLDSRLSIFGPA
                     EVLAEHQIPMASPAATYRDEPCLWLYTSGSTGRPKAVMHAHGSLPAAAELYARRTLGL
                     GSDDIIYSVAKLPFAYGLGASLYFPLWVGASVVLSNATQAFDYVADIHRYRPTVLFGI
                     PSTYESLLAVHELAPLDASSLRLCVAAAEPLPERTFERFRKRHGLAICEGFGTSEMLH
                     IFISNSPDAPRAGWAGKPVTGYRLRVLGDNGREAADGQPGLLEVEGETLMLGYFNRLE
                     QSRRAIRHGAMLTGDRCVRDQHGYFKYLGRVDDLFKVNGQWVTPNEIESVLHEHPAVS
                     ACAVLRDSDDETAGVAAYVALRDGYEASHQLVLEIRSAARAKLPGFMAPRRIEFVQHM
                     PRTATGKIDRGALQRLSKASEVFNG"
     gene            complement(6865..7245)
                     /locus_tag="VFQ61_33835"
     CDS             complement(6865..7245)
                     /locus_tag="VFQ61_33835"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HET9959538.1"
                     /translation="MARFAAKLIGIDVVPVALLERWLARYSDDQLSLVFTPRERSRAK
                     QSGQAAVTLAISYAGKEAVAKALGTGFTRVMPSEIEILSSPFDVRVQLAGAALERAIE
                     RCALGWTGTYWRLGEDIVTTVMTI"
     gene            complement(7271..7906)
                     /locus_tag="VFQ61_33840"
     CDS             complement(7271..7906)
                     /locus_tag="VFQ61_33840"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9959539.1"
                     /translation="MTQTQNAVNRPGVRDYFKQAYSSKPVSQHEWTAGSASPELIKLV
                     WEGVIEPGSRVLDVGCGIGSESVFLAARGMRVTGIDISPSAIEAAKKLAQAYAVEVDF
                     RCADVLNLPSPDAEFDVFCDQGCFHHMADEERAGYVKELVRVLKPGGLFVLRSFSDKI
                     PGGPQPRRISSKELIDTFAGQLRLEHLERVLSFSTPQRERPLGWHSIWLKP"
     gene            complement(7903..8154)
                     /locus_tag="VFQ61_33845"
     CDS             complement(7903..8154)
                     /locus_tag="VFQ61_33845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET9959540.1"
                     /translation="METKLLIKNILLELGVGESDVQPNRLLKRDLQLDSTDTVQIALE
                     IKKRLNVAVRIESSQDLTVAQLCELVDGQKTQSVERISQ"
     gene            complement(8233..>9241)
                     /locus_tag="VFQ61_33850"
     CDS             complement(8233..>9241)
                     /locus_tag="VFQ61_33850"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET9959541.1"
                     /translation="DRFTHMALAAAGMALSDAALSLETEDRYRVGVWFGNNAGGWDIC
                     ERGFHELYRDGATLVNPWQATAWFPTAPQGYVTIRHGIQGMSKSFVCDRASGASALYF
                     AERAIRLGTLDVVLAGGSEAPLTGFGMSCYAGLGELTQRQEPSGAYQPFGVERSGFVL
                     GEGSTVLVLEEGERAKQRGARIYGWLEQGAMGIDPGCDAVDAWTGAFEGCLQRASWQA
                     ADVDVVFGEGAASVAGDRQEAQVLTQVFGARGVPVTIPKTMYGHLYGASAVTELAIAL
                     LSFESGCLPPTTGTQALDPQCPVDLVLEPRSRPVRRALISSRAREGTAVVLGVALEES
                     V"
BASE COUNT         1791 a         3060 c         2736 g         1654 t
ORIGIN      
        1 gcccagtcca actggccttt gcggtgcgac ccgcgtgtat cgcgcagact gcggctgatg
       61 cagtatgccg atatcgatac gaagacgatg ttgatgtgga acgcgtagcg ccaaccgacg
      121 tgctccgcga gcaccgcacc gagcagtggg cccaggatgt tgctgacgcc gtaaacggca
      181 gagaagtttc cctgcagcct aggccgcatc tccggcggaa aatcgaggaa tagcgtcgcc
      241 aacgccgcag gcaaaatcgc gcccgcgcct atgccctgca gaccacggaa caggatcagc
      301 tcgccaaacg atcgtgagaa cgcgatcagc tccgagccca gcaagaacgt gaacaatcca
      361 aacagcagca cgcgtttgcg cccaaaccga tcagagagct tacccaggat tggcgcgctc
      421 gaaatctgca tcagcatgta agatgcgaat acccaaggcg cgagcgggag atccccgaac
      481 tccgacagca tacgcggcaa aatggtcgct acaatcgtac tgtcgattcc cacgaccagc
      541 acacctacca gtagccccgt aagaacagcc ttctgttcgg ctccctcggc gtacgggcgc
      601 cgatccacct gcgcttccat gtctagttca cgatagtacg gtgcagaaca aaacccaaga
      661 gtgtatcgac ttcatgatcc acgcgcgcct tgggatcggg ttaccaagcc tcgtattcca
      721 agtggcgcgg gggtgccgca aacaaggccg cacgcaaaca catctgcgac gcaaagatcg
      781 ctgcgctgca gacttggccg caccgcaact cgtctggatg tgacacgccg caaactctcg
      841 aagctagcct actggggata cggggactca gtcccgatct cgacggggag catcgcattc
      901 acattaaaaa cctttgaacc tcgcgccgcc acctgaaata tctttgcgtg acgtttacgc
      961 actcagcgat gttccgcgcc gattctgtgg ctcacgtgcc cttccaagag tcttggataa
     1021 ctcctgccag cgacgaaacg acgacgacct ttcgaccagc agagctatcg cgagcacgat
     1081 gggcgccgag atggccgaaa gatgcaagga atgggtcatc gccgaccagc ccaattggga
     1141 tgggctctag gcgccctgct cacgctgtag tcgttcggcc caatcgtgaa actcgcgagc
     1201 ggcctgccgg ggctgctcca agtgtacgta gtgcccggcg tcgttcaggg tgacgcaggt
     1261 gcctcgcggc acgcccgccg ccaatgaacg cgagtgccgg ggggatacga tgcggtccga
     1321 accggcgcca atcgcgagca gcggcactct caggttcggg agcgccgctc gcacgtccaa
     1381 gtgttcaccg tcgaacatcg acgcgggtcc aaagcggacc ttgcacaggt cgtcgtagac
     1441 gagctctgcc caggggtcat cggatcgcgc gaaccctccg cgtgcgagct cgtactcgaa
     1501 tcggccgtgg cgagcctgct gaacgaagtc cggatgaagt tgcaagcggg gcgccgaagc
     1561 aatcgtgcaa aggccgctca atcgcttcgg gtagtgcagc gccaagctca acgccaaaac
     1621 cccacctgcg gagtgaccaa tgcaccagag tggggtcgga agccgtaacg cctcgaacaa
     1681 ctccgccagg aattcgacgg cagcgtcgag cgacggcgcc accgtgccgt ccgattcccc
     1741 gtgaccgggc agatctaccg cgatcggtac gaagccggga gcgagcgcct cgatgaaggg
     1801 gccccactgg cggtgactcg cacccgcacc gtgcagcaac aacaccgacg aggagcccac
     1861 ccgagccgcg ccccgcggca gcgcgacccg tatccccgtg tctcgaattc gaaggcgctc
     1921 catcgtctca gctgctcgcc agcgtgacgt gcgtagccat ggtcttcatc ttgttcacgg
     1981 actccatgta ttccatttcg ggccgagatt ccgcgacaat tccggccccc gcgttcaaca
     2041 cggtgcgctg gtcatagccg tacacggagc ggatcgcgag acacagatcc gcggccccgc
     2101 tttgatcgat ccagcccacg gcgccgccgt agactccgcg cggcccctcc tcgagctgac
     2161 caatcgcctt gagggcagcg gccttgtcga ctccactcac cgtaatcgcc ggaaacagga
     2221 tcttggccgc gtcccaagtc gtgaaccccg ccttcaactg gccgctgacg cgcgacgaga
     2281 ggtgctgaac gcatctaaac tgcttcacgt ccatgaagtc gtagatgcga accgtctcgg
     2341 cgtgacagac ccggctcatc tcctcctggg ccaagcgcac cgacatcgcg tgttccttca
     2401 cttccttgct gtccgcgacc agctcggctc tcacccgctg gtcctcgtcc aaggtccccc
     2461 ctcgcggccg cgtgccggca agcgggttcg tgatgacttg accgtcactg gttgcctcca
     2521 ggaacaactc cgggctgaat ccgacgccgc ggacgccgct cagctcgaag cagaaagagc
     2581 gagccgcgtt gttggcgtca cgcgcattca ggtaggtacc caagacatcg agttccccgg
     2641 gcacctcgac gcgccgggac aggatcgcct tctgcaattt gccttcttgg atccagccaa
     2701 tcaaggtctg cacccgcgcg acgtactctt gatgatcgct cgaaccgggc tgcggcggcg
     2761 ttgcgctctg gggatgacgc gtcgcttttc gggccgcgtc gagcacatct ttccagacct
     2821 gggccggcaa cgccgtcgtg acccgcaccg agtctcgcgc gatacgtagc tccgtctcgg
     2881 gaatcatgaa gaacaaagac ggataaccag tgtccttccg gtagtcgaag tagaagcggg
     2941 ctaggtcgaa gccaatatac ccatatgcgg tccaatttcg gagcgggaca tcggccaacg
     3001 ctcgctccac ctgcttcaac ggatcttcgg cgcgcacgcg ggagtgcttc tcgggctgat
     3061 cgccgtcgag ctcgactacg tctcgactga cgcgtacgcg cgctcgcgag ttgagcgcaa
     3121 caatcgtctc accctcgccg gtgtgattac gatacatcag ataatcgcga accaacccct
     3181 gcactttcaa gtgctcgacg agggcggtcg gatcgacgga gtgcctgaat tcgaacacgt
     3241 accgttcagc cgacatggct ctgctccttt ccgctccagc tctccaaacc acgcttcacg
     3301 gaggccattt gtacgcgggt attgcgattc aagaagctct ccatgcgctc ctcgctccaa
     3361 gggcaatcgg cgtggacctc gaaatcctga atccacgtca tgaccacccc gacctctttg
     3421 ggcagtcgct cgtagaccca gcggatcttc atgtatgcga aagggaacgt cgggtcgagg
     3481 cgctccgccg tggccacgcc agccgcgcgg tccaccttac gccgcgacgt ccaagtgcgc
     3541 gcgggtcgcc cgtcctcagg gtgagtcgtc agtcgaaaga ggatctcatc cccgactcgt
     3601 tccagcacct ccgccttgga gtactcggtg aacagcaccg gccaaagctc cacgcgattc
     3661 gagacgtcca gcacggtctc gtagtcgcgg agcacgacaa tcgtattttc agtatgacca
     3721 gccattggat ctccggagtc agttgttcgc ttcgggcagg atgcccacgg ctctgaccca
     3781 gctcgcgtcg agcccgcgga tcttcagcgg gaagcaagcg atgtcgaagc ctgtgggcgg
     3841 cagtttatcg aggttcgcga ggcgctcgat ctgaatgtac tctcgttctc gaccatagaa
     3901 gtgcgccggc cagaggtgcc ggggatcgcg agttcggtag aagtcgccca gcatcgcgcc
     3961 gaagggtcga tcgaacccgt aggtatcgat gccgatgacc cggatcccgc gctctacgag
     4021 ccactctgtc gctgcgcgcg acatcccagg agcatcgctg aagtattcgc gcttacccca
     4081 gagccgatcg gtgccggtcc aaatcaagac gatatcgccg cggcggagtt cgtgccccga
     4141 ggcctgcagg gcgtgctcga gatcctcggg ctggatgtat tgcgccttgc ctttgtgtct
     4201 taggtccagc cgcacgccgc gcccaaagaa ccattcgagc ggcagctggt cgacggagcg
     4261 agcaggagta ccatccgagg tcgggccgta gtggacgggg gcgtcgacgt gcgtgcccat
     4321 atgggtcggc aacttgacgg tgtccagggt caggaacgcc cgccccggaa agtccttggg
     4381 caggatccgc gctttgcccg agacgaagcg caacgcctgc tgcacgcgct tgggaagcgg
     4441 caaacgccgg ttccagaagg cctcgaagca gaagtgtcgg gccccgcgca ccggatccag
     4501 ccgctggacc tcgatccgca ttggctccga gggcgaattc tcgaccagca cactcaaatc
     4561 aacgagctgt gccatcgcga cttcggccct ccttggactg gatggttcgg atcgcgacct
     4621 ccaaggcttc gttgactcga ctgatgccga ccaccggcaa cgccagcgtg atcgcggcca
     4681 cgatttcttc gcgcgtagcg ccgtgctcct gggcccgctc gacgtgagtc tgaatccctc
     4741 gcgttccccc gtgcgccacg aacactccga ggatcacgag ctcattgagc ttggggccga
     4801 gcggagaggc gtctcggatc gatttggtca gagcgaagaa ggactcggct acgggtgcgg
     4861 cgttttcgat tagatgttgc atgttcagga tcctttctcg agatttcggt gtccgctttc
     4921 aggtcgcggc gcgagcggag cggatgcgaa gacgaccacc acctcctccg agccacggtt
     4981 ctcgatcgaa tgggtcactc ccttggggac cagcaccgcg tccccggccc ggaactccac
     5041 cgtgccgatg ccgtccagaa tcgcgcagcc ggtgccctgc acgacgaaga aggcctcttc
     5101 gctgtagtca tggacgtgag ctttgactcg ctccccgggc ggcacggtgg ctttccccag
     5161 gatgagctgt ttggcgttca ccgagccggg agtgatcatg acccgatagg tgccgccgcg
     5221 atcggtgcgc tccgtcacct ctcgctcact gatgcataac ttggaatcag ccattgaaga
     5281 cctcacttgc cttggataag cgctgcaatg ctccgcgatc gatcttgccg gtcgcggtgc
     5341 ggggcatgtg ttgaacgaac tcgatgcgac gcggagccat gaacccaggc agcttggcgc
     5401 gcgccgcact ccgtatctcg agcacgagtt gatggctcgc ctcgtagccg tcacgcaacg
     5461 cgacataggc ggctacgcct gcggtttcgt cgtccgagtc gcgcagcacg gcgcatgcgc
     5521 tgacggcagg atgctcgtgc agcacgctct cgatttcatt tggcgtaacc cactgcccgt
     5581 tgaccttgaa caagtcatcg acacggccga ggtatttgaa ataaccgtgt tgatcgcgga
     5641 cacagcgatc gccagtaagc atggcgccgt gccgaatcgc gcgccgactt tgctccagtc
     5701 gattgaagta acccagcatc aacgtctccc cttcgacctc gagcaaaccg ggttgaccgt
     5761 cggccgcctc gcgcccgtta tcgcccagta cgcgcaaccg atagccggtc acgggtttgc
     5821 cggcccagcc cgcgcgcggc gcatcggggg aattcgaaat gaagatgtgc agcatctcac
     5881 tcgtgccaaa gccctcgcaa attgccagcc catggcgctt gcggaagcgc tcgaacgtgc
     5941 gctccggcag cggttccgcg gccgccacgc acaaccgcaa cgaactcgcg tcgagcggcg
     6001 ccaactcgtg caccgcgagc aggctctcgt aggtgctcgg gatcccgaac aacaccgtcg
     6061 ggcgataacg gtgaatgtcc gccacatagt cgaaggcctg cgtcgcattc gacagcacca
     6121 cgctcgcgcc gacccaaagc ggaaagtaga gactcgcccc gagcccgtaa gcaaacggga
     6181 gctttgcgac ggagtagatg atgtcgtcac ttccgagtcc caacgtgcgg cgcgcgtaga
     6241 gctccgctgc ggccggcagg gatccatgcg cgtgcatcac cgccttgggc cggcccgtgc
     6301 taccagacgt atagagccac aagcagggct cgtcccgata agtcgcggcc ggcgacgcca
     6361 tcggaatttg atgctccgcc aacacctccg cgggcccaaa gatgctcagt cgagagtcga
     6421 ggctcgcgga aagacgagcc gagcctggtt catcgatcaa gagcgcacgg gcccggctct
     6481 cttcgaggat gaattcgatg gtctcgctgc tgtaagcggt gttgatgggg accgccaccg
     6541 cgcccatcca aatcgcggcc agaatcccga tcacgagctc cgggccgtct ggcagcagga
     6601 gcgcgacccg gtcttcttga cggactccag ccgcgagcag gcgcgccgca aagcgcgcga
     6661 ccgcttccgc gagctgcccg tgggtaaagc ttcggtcccc acagatcaac gcccgggcgg
     6721 cggcgcgctc gcgattcctc tgcagaagag ccgctgcgac attgaaggag cgtggcaaag
     6781 tcgcggctat cgagtccgaa acgccgcggg tcgcggagtt cgcgttgagc gacactccac
     6841 tcttgaagtc ctggactagt gaagtcatat cgtcatcacc gtggtcacga tgtcttcgcc
     6901 caagcgccag taagtacctg tccacccaag ggcacaccgc tcgatcgcac gctcgagcgc
     6961 cgcgccggcg agttgcacgc ggacgtcgaa cgggctggag agaatctcga tctcactggg
     7021 catcacccgg gtgaaaccgg tgcccagggc cttggcgacc gcctctttgc cggcatagct
     7081 gatcgcaaga gtcacggccg cctggccact ttgtttcgcg cgggaccgct ccctgggcgt
     7141 gaacaccagg gatagctgat catcggagta gcgagctagc cagcgttcca gcaacgcgac
     7201 cggaacgaca tcgatgccaa tgagtttggc ggcgaacctg gccatcagcc cggcgctcgc
     7261 ggtcgctaat tcagggcttc aaccagattg agtgccaacc cagcgggcgt tcgcgctgcg
     7321 gtgtggagaa ggagagcacg cgttcgaggt gctcgagccg cagctgaccc gcgaaggtat
     7381 caatcagttc tttgctcgag atgcgccgcg gctggggccc gcctgggatc ttgtccgaga
     7441 aggaacgcag cacgaacagc ccgcctggct tgagcacacg taccagctcc ttcacgtatc
     7501 ccgctcgctc ttcgtccgcc atgtgatgga agcagccctg gtcgcagaac acgtcgaact
     7561 ccgcgtctgg gctgggaaga ttgaggacat ctgcacatcg aaagtcgact tcgaccgcgt
     7621 acgcctgtgc gagcttcttg gccgcctcga tagcactcgg cgagatgtcg atacccgtga
     7681 cccgcatccc gcgcgcggcc aggaacacgg actcactacc aatcccgcaa cccacgtcca
     7741 acacccgaga gcctggctcg atcacgccct cccaaaccag cttgattagc tcgggcgaag
     7801 ccgaacccgc agtccactcg tgctgcgaaa cgggtttgga actgtatgcc tgcttgaagt
     7861 agtcgcgaac tccgggccta tttactgcat tttgcgtctg cgtcattgag agatcctttc
     7921 taccgattgc gttttctgtc cgtctacgag ctcgcagagc tgcgccacgg tcaagtcttg
     7981 tgaagattcg atgcgtaccg cgacattgag tcgcttcttt atctcgagtg cgatttgcac
     8041 ggtgtcggtg gagtccaact gcagatcacg tttcagcagc cggttgggtt ggacgtcgga
     8101 ttctccgact ccgagctcga gcaggatgtt cttgattaat agtttcgttt ccaagatgac
     8161 gatctccctg agttctgccg cgccgattgg tagcgccacg agcgtgattg aacgccgcgg
     8221 actcgcgtcc gctcagaccg actcttcgag ggcaacgccg agcacgaccg cggttccctc
     8281 tctcgcgcgc gagctgatca gagcgcgacg caccggcctc gagcgaggtt cgagcaccag
     8341 atcgaccggg cactgcggat ccagagcctg ggtccccgtc gtaggaggca aacagccaga
     8401 ctcgaagctg agcagggcaa tcgcgagctc cgtgacggcg ctcgctccgt acaagtggcc
     8461 gtacatcgtc ttgggaatcg tcaccggcac cccacgtgcg ccaaacacct gcgtcagaac
     8521 ctgcgcctct tgtcgatctc ccgccacgct cgcggcgccc tcaccgaaca cgacgtcgac
     8581 gtctgcggct tgccaactcg cgcgctgcag gcagccttcg aacgcaccag tccaagcgtc
     8641 caccgcgtcg cagccgggat cgatccccat cgcgccttgc tccaaccagc cgtagattcg
     8701 ggccccgcgc tgcttggccc gctcgccttc ttccagcacc aagacggtgc tgccttcccc
     8761 gagcacgaag ccgcttcgct ccactccgaa aggttggtat gcgccgctgg gttcctggcg
     8821 ctgggtgagc tcacccaaac cggcgtaaca gctcattccg aagcccgtca gcggcgcctc
     8881 cgagccgccc gcgagcacca catccagtgt accgagtcga atggcgcgct cggcgaagta
     8941 gagcgcggac gcgccacttg cacggtcaca cacgaagctc ttgctcatgc cctggatgcc
     9001 gtgccgaatc gtcacgtagc cttggggcgc agtcggaaac caagccgtcg cttgccaagg
     9061 gttgacgaga gttgcgccgt cccgatagag ctcatggaac ccacgctcgc agatgtccca
     9121 gccgcccgcg ttgttcccaa accatacccc tactcgataa cgatcctccg tctcgagcga
     9181 cagagccgcg tccgacaacg ccatccccgc tgccgccagc gccatgtgcg tgaatcggtc
     9241 g
//
LOCUS       DASTKU010000954         4160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1080004, whole genome shotgun sequence.
ACCESSION   DASTKU010000954 DASTKU010000000
VERSION     DASTKU010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080004"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /gene="ilvY"
                     /locus_tag="VFQ61_33855"
     CDS             complement(<1..853)
                     /gene="ilvY"
                     /locus_tag="VFQ61_33855"
                     /inference="COORDINATES: protein motif:HMM:NF008722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HET9959542.1"
                     /translation="MAPAIDLARMDYDALRLFLKLSRTLHFTRTSRASGVSLSGLSRT
                     IQRLEEEVGQPLFVRDQKTVTLTPAGQIFAEHAAETLARFESLKQRLQDSGEKLSGTI
                     SIFASVTAVQSFLPRVLSRFRTSYPEVHIELETGHATDALANLEQGRVDVTIAALPDR
                     IPPALEARVIVVTPLVMVAPASDCDVSRSLEKKPVAWGEVPMVFPAAGLARAAADRWF
                     SRRRLRPKVYGEVSGNEAILSLVSLGCGVGIVPRLVYEKSPLKQDLRLLDVEPRLSDF
                     RVGVCVER"
     gene            complement(878..2353)
                     /gene="pap"
                     /locus_tag="VFQ61_33860"
     CDS             complement(878..2353)
                     /gene="pap"
                     /locus_tag="VFQ61_33860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HET9959543.1"
                     /translation="MFESAELGHSLDKATYDRELPALRAGLLAAQYELLKSASFPVII
                     VVAGVESAGKGDTVRALSEWMDPRHLKVHAMGPPSDEELERPPMWRFWRRLPPKGKIG
                     VFFGSWYTAPITSHALGKRGKHPFADALGEIRRFERMLFDEGALILKFWYHLSKQVQR
                     ERLEQLWQDKKTRFRVSKTDWKRLKRYDDFRSTSERALIETDVPEAPWLVLEGADSRY
                     RNFNTGKAIHDALQKRLAVPTAERTVGRVIDVPNVQHVPLVRTMDLSVKAEKPEYERE
                     LGELQRRLTLLTLEKRFRKISPVIVFEGMDAAGKGGAIRRLTQAVDARTYDVHAIAAP
                     TEEEKLQPYLWRFWRNVPRDGKFAIFDRSWYGRVLVERVEGFADEPTWLRAYGEINDF
                     EAQLERAGNVVIKFWLQISNAEQLRRFEEREKTDFKRFKITEEDWRNRNKWEAYELAA
                     SQMIERTSTEQAPWTLVEAEDKYFARLKVLRTTCEAIERAL"
     gene            3188..3793
                     /locus_tag="VFQ61_33865"
     CDS             3188..3793
                     /locus_tag="VFQ61_33865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HET9959544.1"
                     /translation="MDILTELRSATAAVHRRLELDLALLGPGLTRSRYEAFVCGMAGM
                     ILPFESSWDEIPGWGRVLPDYESRRKAGLLLADIQRFQPAPQSPLPLAPGFVAQDCET
                     ALGVGYVLEGSTLGGAVIAREVLQRLGPHCPAAYLQCYGSGVGTKWKGFCSALEQHCG
                     DAASALTPSERAARAERVVLNAVQTFDRARDWLNRDEALSA"
     gene            3790..>4160
                     /locus_tag="VFQ61_33870"
     CDS             3790..>4160
                     /locus_tag="VFQ61_33870"
                     /inference="COORDINATES: protein motif:HMM:NF020035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959545.1"
                     /translation="MIELQECETEPIHTPAAIQPHGALLAFAFGRGLDRNGNRTGGTG
                     PYRATPDYAELSGSFEPDLILQQFSANAGELLGLTEPLRCDQGIRDILPAHATALREA
                     ARALSDSTPREQQTLRVSNDRG"
BASE COUNT          836 a         1298 c         1219 g          807 t
ORIGIN      
        1 gccgttcgac gcagaccccg acacgaaagt cagagagccg cggttccacg tccaacaagc
       61 gcaaatcctg cttgagcggg ctcttctcgt agacgagccg cggcacgatt cccacaccgc
      121 aacccaagct taccaaagac aggatcgcct cgttcccact gacctcgccg taaaccttcg
      181 gacgcaatcg acggcgcgaa aaccaacgat ctgcagccgc tcgtgccaac cccgcggcgg
      241 gaaataccat tggcacctca ccccatgcga cgggcttctt ctccaaactt cgactgacat
      301 cacaatccga agctggagcc accatcacca aaggcgtgac cacgatcacg cgcgcttcga
      361 gcgcaggagg gatgcgatcg gggagagcgg cgatggtgac gtccactcga ccttgctcga
      421 ggttggcgag cgcatctgtc gcgtgtcccg tctcgagttc gatgtgcacc tccggatagc
      481 tcgtgcggaa gcgggagagc acgcggggta agaagctctg aacggccgtc acggaggcaa
      541 agatcgagat cgttcccgag agcttttccc cagagtcttg caagcgttgc ttgagagact
      601 cgaaacgtgc cagtgtctcc gcggcgtgct ccgcaaagat ctgccccgcg ggagtgagcg
      661 tgaccgtctt ctgatcacgc acgaacaggg gttgaccgac ctcttcttcc aacctttgaa
      721 tggtccgtga aagaccagat aagctgactc cactcgcccg actcgtgcgc gtgaagtgga
      781 gagttctcga aagcttcagg aacaatcgta gcgcgtcata gtccatgcgt gccaaatcaa
      841 tagcgggtgc caaatcaaca gcggtggccg atgcctctca caaggcgcgt tcgatcgcct
      901 cgcaggtcgt gcgcaacacc ttcaggcgcg cgaagtactt gtcctccgcc tcgaccaatg
      961 tccacggcgc ctgctcggtg ctggtacgtt cgatcatttg gctggccgcg agctcgtaag
     1021 cctcccattt gttgcgattg cgccagtcct cttcggtgat cttgaagcgc ttgaagtcgg
     1081 tcttctcgcg ctcctcgaaa cggcgcaatt gctcggcatt gctaatctgc agccaaaact
     1141 tgatcactac gtttccggcc cgctcgagct gggcttcgaa atcgttgatc tcgccgtaag
     1201 cgcgcagcca agtaggttcg tcggcgaaac cttccacacg ctctacgagc acgcgcccgt
     1261 accaactccg atcgaagatc gcgaactttc cgtcgcgcgg cacgttgcgc cagaagcgcc
     1321 acagataggg ctgaagcttc tcctcctcgg tcggcgctgc gatggcgtgc acatcgtaag
     1381 tgcgggcatc cacggcttga gtcaggcgtc gaatcgctcc tcctttaccg gccgcgtcca
     1441 tgccttcgaa cacgatcacg ggagagattt tgcggaaacg tttctccaac gtcagcaacg
     1501 tcaacctgcg ttgcaactcc ccgagttcgc gctcgtactc cggtttttct gcctttacgc
     1561 tcaagtccat cgtacgcacg agcggaacgt gttgaacgtt ggggacatcg atcactcgcc
     1621 ccaccgtccg ctccgcggtt ggaacggcca gtcgcttctg gagcgcgtcg tgaatggcct
     1681 ttcccgtatt gaagttgcga tatcgagaat ccgccccttc gagaacgagc cagggtgcct
     1741 cgggtacgtc ggtctcaatc aaagcacgct cactggtgct tcgaaaatcg tcgtagcgtt
     1801 tgagacgttt ccagtctgtc ttgctcaccc ggaagcgagt tttcttatct tgccatagct
     1861 gctccagccg ctctcgctgg acttgtttcg acaggtgata ccaaaacttc aaaatcaacg
     1921 cgccctcgtc gaacaacatc ctttcgaagc gccggatctc cccgagcgcg tccgcgaacg
     1981 ggtgcttgcc gcgtttaccg agcgcgtgcg aggtgatcgg cgccgtgtac caagagccga
     2041 aaaacacgcc tatcttgcct ttcggtggca agcgtcgcca gaaacgccac atcggagggc
     2101 gctccaactc ttcgtcgctg ggagggccca ttgcgtgaac cttcaagtga cgcggatcca
     2161 tccattcgga caaggcgcga accgtgtcac ccttaccggc gctctctacg cccgccacca
     2221 cgatgatcac ggggaagctc gcgctcttca gcaattcgta ctgggcggcc agtaacccag
     2281 cgcgtaacgc aggaagctca cggtcgtacg tagctttgtc caaggagtgt ccgagctcgg
     2341 ctgattcgaa catgcaaagg accatacccc aaaacgcgag gcaggtttgc tggtgtatgc
     2401 tgcgggccgg aacatgaaga aaaaggcgcg cagtgagagc gagcgaacgc ggcggcgtac
     2461 tgcagcccgg agccaaaacc gcgcgcagga ggaaagagag cggctgttca gacaaagccc
     2521 gggaggcagc cccgagcgtc cgatcctggt cagctcggct tcggtcgtcg aggctcacgc
     2581 caagcatctt tcgtgtccgc aatgtggtgg gtcgcagcgg gtactcgagc actccgccat
     2641 cagcatcgag acagttcggt tacgccgagt agagctcacg tgcgcgcagt gcggaacgcg
     2701 cagatcggcc tattttcgct tagacactgc gttaccgaat tgaaccgctc ttccaactcg
     2761 agctgcgctt tcgaaaatgg ctttctaact tggaacccga aacatagcgg cccgtcacgg
     2821 cgagggaccg cgtgtatctc gagactacgg acatcaaatc aatgcacgat ggctgaattg
     2881 gtgtgcattg gatcgccgaa cgtgcgttcc acgcgtgcgg agtgagtgag ctcggcaacg
     2941 agccacggcc ccgcgggtga accgaaaaga attcgagcat tcttccggca ggaatgccga
     3001 aaacggcacg aactacattt tgtacaactc ttgcgacacg tggcgagcca aaccaagctc
     3061 acccgagtgg cgctcatcgg ttggcgagag aacgaccgga gagcgctaag agagtacgcc
     3121 cttcgcggct gcgtagatac tggcccgccg ggtgctgcgc tcgacgactg acgcaatggt
     3181 tttctagatg gatatcctga cggagcttcg tagcgcgacc gcggccgtgc atcgccgcct
     3241 cgagctagac ttggcgctac tcggcccagg cctcacgcgc agccgatacg aggcgttcgt
     3301 gtgcgggatg gcgggtatga ttttaccttt cgagagctcc tgggacgaga ttcccggttg
     3361 ggggcgggtg ctgccggact acgagagccg gcgtaaggcg gggctcctcc tcgcggatat
     3421 ccagcgcttc caacccgcac cccaatcgcc cttgcccctc gctccgggat tcgtggccca
     3481 agattgcgaa accgcgctgg gcgtaggcta cgtgctcgag ggctccacgt tgggcggcgc
     3541 ggtgattgca cgcgaagtgc tgcaaaggct cggccctcac tgcccggctg cctacctcca
     3601 gtgttacggc tccggagtcg gcacgaagtg gaagggattc tgtagcgctc tcgagcagca
     3661 ttgtggcgac gctgcgtccg cgctcacgcc ttccgaacga gcagctcgcg ccgaacgcgt
     3721 cgttctcaac gcggtccaga cattcgatcg agctcgcgat tggctcaacc gcgatgaggc
     3781 attgagcgca tgatcgagct acaggagtgc gagacagagc caatccacac tccggcggca
     3841 attcagccgc acggcgcgct cctcgccttc gccttcggcc ggggactcga tcgaaatgga
     3901 aataggaccg gtggaaccgg accttatcgc gctactccgg actacgccga gttgtcgggt
     3961 tcgttcgagc cagacttgat tcttcagcag ttcagcgcga acgccggtga gttgctgggt
     4021 cttacggaac ccttacgctg cgatcagggg attagagata tcttgcctgc gcacgcgact
     4081 gccctccgcg aggcggcgcg tgcgctttcg gattccacgc cgcgcgagca gcagacgctc
     4141 cgcgtatcga acgaccgagg
//
LOCUS       DASTKU010000955         3884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1750604, whole genome shotgun sequence.
ACCESSION   DASTKU010000955 DASTKU010000000
VERSION     DASTKU010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750604"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..430)
                     /locus_tag="VFQ61_33875"
     CDS             complement(32..430)
                     /locus_tag="VFQ61_33875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959546.1"
                     /translation="MDEEEKTCGQELAESANVPEQFAQLFQHVAFNLRDHAAWVGTAS
                     AIARREHDALLAVAHGYDAVASEATRTASLMRAMHDIPSAPHDPESWDQARFLDWMRQ
                     KVVLQRRLARLLLEHAEQSEQVLGQLDGSQ"
     gene            complement(549..1187)
                     /locus_tag="VFQ61_33880"
     CDS             complement(549..1187)
                     /locus_tag="VFQ61_33880"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET9959547.1"
                     /translation="MLKVYGFARVNEGARGNTRDLRVLWALEEIALPYEIVGMDHPNH
                     DLDSPAYRALNPFGQIPVIDDDGVVVSESGAILLYLARKSGKLIPRDLAGEAQVLRWC
                     FTALTTIELPILSLWFVNLCGGKGSKPSDALQGWGDMRLKQLDGWLEERQFIATDDFT
                     VADILMSHVLDAGTDPERLKPHANLLAYRARCTERPAWKKTLEAYYERVKPA"
     gene            complement(1462..1974)
                     /locus_tag="VFQ61_33885"
     CDS             complement(1462..1974)
                     /locus_tag="VFQ61_33885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9959548.1"
                     /translation="MEARSEIARSPMKNSATVERKSDRELVVTRTVNGPARLVFEAWT
                     KAELFKRWWVPKSCGVTLLSCELDVRVGGKYKLVFAHGDSTMDFFGKYLEVVPHSRLA
                     WTNDEGDDGGAVTTVTFDEKAGKTQVIVHDLYPSKEALDEAVASGSTSGMPETLDQLD
                     ELLVTLSSNG"
     gene            complement(1958..2317)
                     /locus_tag="VFQ61_33890"
     CDS             complement(1958..2317)
                     /locus_tag="VFQ61_33890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET9959549.1"
                     /translation="MVQCSTAHLDGSFAALSDATRRGVLEQLSRADASITDLADKFHM
                     TLTGMKKHVGVLEQAGLVTTKKIGRVRTCRLGPRRLEDEAAWIERYRQLWDARFDELD
                     DVVEELKLKEKMNGSKK"
     gene            2673..2963
                     /locus_tag="VFQ61_33895"
     CDS             2673..2963
                     /locus_tag="VFQ61_33895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959550.1"
                     /translation="MSAPDIARDCGSVFPLVLPIKLSDSELLSATRRLVASSNQLLAL
                     LLVHLAEVEDRGLHRTRACSSLYTYCMYELRFSEDQAFRRVAAARARSEPTA"
     gene            2986..3264
                     /locus_tag="VFQ61_33900"
     CDS             2986..3264
                     /locus_tag="VFQ61_33900"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HET9959551.1"
                     /translation="MRGSLSASAGYVPAPVRRAVFERDQGRCTFIDGSGRRCRETHRL
                     ELHHLKAFARGGEHTLDNLALRCRAHNALAAEQDFGADFIQQRRESSR"
     gene            complement(3292..3612)
                     /locus_tag="VFQ61_33905"
     CDS             complement(3292..3612)
                     /locus_tag="VFQ61_33905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959552.1"
                     /translation="MRLHEVQGLAFVTWPKEDSAAPQVLHDITAIFVPPLGVLSGEAR
                     GEGVEVVCNQPSPLTEHCGGTLSFRATAAQVSDQNGNLHSLESLASVADAYWKDWSAR
                     TPKS"
BASE COUNT          727 a         1237 c         1159 g          761 t
ORIGIN      
        1 ggcaggacgg cattattggt tctccgcgag tctactgaga gccatcgagt tgaccgagga
       61 cctgttcgga ttgctccgcg tgttcgagca ggagcctggc gagcctgcgc tgcaaaacga
      121 ccttttgcct catccaatcg agaaaacgcg cctggtccca actctccggg tcgtgcggcg
      181 cagaggggat atcgtgcatg gcgcgcatga ggctggccgt gcgagtcgct tcgcttgcaa
      241 cggcgtcgta gccgtgagcc acggcaagca gcgcgtcatg ctcgcgccga gcgattgcgg
      301 aagcggtgcc gacccaagcc gcgtggtctc gcaggttgaa ggcaacgtgt tgaaacagct
      361 gagcgaactg ctccggcacg ttcgcactct ctgccagttc ctgcccgcaa gtcttttcct
      421 cttcatccac ggcgaaccac tcctccgcaa tcgcgtcctg aagcgcgacg ttagcacgag
      481 ccctccattc ggtggaattc gataaaccac gagttcttgg agcgtggcct ttgttgctcg
      541 tcggcctctc aagccggctt gacccgctcg tagtacgctt cgagcgtctt cttccacgcg
      601 gggcgttcgg tgcaccgggc gcggtatgcg agcaggttcg cgtgcggctt cagcctctcg
      661 gggtcggttc ccgcgtccaa cacatggctc atgaggatat cggcaaccgt gaaatcatcg
      721 gtcgcgatga actgtcgctc ttcgagccag ccatcgagtt gcttcaaccg catatcgccc
      781 cagccctgga gcgcgtcgct cggcttgctg cccttgccac cgcacaagtt gacgaaccac
      841 agcgacagga tcggcagctc gatcgtggtc agcgccgtga agcaccagcg aagcacctgg
      901 gcctcgcccg cgagatcgcg gggtatcagc ttcccgctct tccgcgcgag gtagagcaga
      961 atcgcgccgg actcggatac cacgacgccg tcgtcatcga tcaccgggat ctgaccgaac
     1021 ggattcagcg cccggtaagc gggtgaatcg agatcgtggt tcggatgatc catccccacg
     1081 atctcgtagg gcagcgcgat ctcttcgagc gcccatagca cgcgcaaatc gcgagtattt
     1141 ccccgcgcgc cctcgttcac ccgagcaaag ccgtacacct tcagcatgcg cgcagcctac
     1201 cgcgatcgat cgagcatgtc tcggcgcagt cagacgagat cgatgaatga ggcaaagtgg
     1261 ttacgcatgc ctcaagggcc catcggggtg acccccctca ctcgaatcga ttcgctaggt
     1321 cgatgtatga actcgcgagc gagcactccc gccggggaaa ggcggttcgc gacccgcgtt
     1381 cagcagcgtg gccctgagcg caacgggcca cgtctgcttc aacaccaagt taacgagcgc
     1441 cggtagacga cgtcacactc atcacccgtt cgagctgagc gtgacgagca gctcgtctag
     1501 ttggtcgagc gtctcgggca tccctgacgt ggagccggag gcgacggctt cgtcgagagc
     1561 ctccttcgat ggatagagat cgtgcacgat aacctgggtt ttccccgcct tctcgtcgaa
     1621 agtcaccgtg gtaacggcac cgccatcgtc accttcgtcg ttcgtccacg cgaggcgcga
     1681 gtgagggacg acttcgaggt acttgccgaa gaagtccatg gtggagtcgc cgtgggcaaa
     1741 taccaacttg tacttgcccc cgacccgaac atccaattcg caggaaagca gggtcacacc
     1801 acacgacttc ggtacccacc agcgtttgaa gagctcggcc ttggtccagg cctcgaacac
     1861 caggcgcgcg gggccgttca cggttcgcgt caccacgagc tcacggtcgg atttccgctc
     1921 cacggtcgcg ctgttcttca tcgggctgcg agcgatctca cttcttgctt ccattcatct
     1981 tctccttgag cttcagttcc tcgacaacgt cgtccaactc gtcgaaacgg gcgtcccaga
     2041 gctggcggta tctctcgatc caagcggcct cgtcctccag ccgtcgcggc ccgagcctgc
     2101 acgtccgtac gcgaccaatc ttcttggtgg ttacgaggcc ggcctgttcc aagacgccga
     2161 cgtgcttctt catgcccgtg agcgtcatgt ggaacttgtc cgcaagatcc gtgatcgaag
     2221 cgtccgcacg actgagctgc tccagcacgc cacgccgcgt cgcatccgaa agcgcggcga
     2281 aagagccatc caggtgggcc gtcgaacact gaaccatgtg gttcactatt aacgcgccgg
     2341 tccgtaccac gcaagtggga aacggatcgg agcgctacct tcacggctgc ctcccccttg
     2401 aatgaagctc gtgcccacgt acgggtgggg cgctcaagct atctcgcacc agttgccgag
     2461 tcgctcggcg atttcacatt cgctgcttga cacgattggc gagtgccggc gccactcggc
     2521 gcgttgatgt ggcttttctc gcgtctgccg cgaacacgtg gcccggagtg ctgctttcgc
     2581 gcggggagaa cgttcgaagc tcatatctgc gagtgaaaac gcgcgagggc gcgaatgggg
     2641 agcacagaaa atcacggggt gaagtctcgt tcatgagcgc accggacatc gcccgcgatt
     2701 gcggttcagt gtttccgctg gtcctaccga tcaagctttc cgacagcgag ttgctcagcg
     2761 cgacgcgtcg cctcgtggct agctccaatc agctgctggc gctgctgctc gttcacctcg
     2821 ccgaggtgga ggatcgcggg cttcaccgca ctcgggcctg ctcgagtctc tacacgtatt
     2881 gtatgtatga gctgcggttc tcagaagacc aagccttccg ccgggtagcg gccgcgcgcg
     2941 cgcgtagcga gcccaccgcg taagtgatcg aaattgatcc actttatgag gggatccctc
     3001 agcgccagcg cgggatacgt gcctgcgccc gttcggcgtg ccgtcttcga gcgtgaccag
     3061 gggcgctgca cgttcattga tggttcaggg cggcgttgcc gagagacgca tcggctcgag
     3121 ctccatcacc tgaaagcctt tgcgcggggc ggcgagcaca cgttggacaa tctcgcgctg
     3181 cgctgccgag cgcacaatgc actcgccgcg gagcaggatt ttggtgcgga cttcatccag
     3241 cagcgtcgcg agtccagccg atagggctca agccaattag gccagtcgcc gtcagctctt
     3301 gggagtccga gcgctccagt ctttccagta ggcatcggcg acgctcgcaa ggctctccaa
     3361 cgagtggaga ttcccattct ggtcgctgac ctgggcggcc gtagcccgga acgagagtgt
     3421 gcccccgcag tgttcagtga gcggcgaagg ttgattgcag accacttcca ctccctcgcc
     3481 acgagcctca ccgctgagaa cgccgagcgg tgggacaaag atggccgtaa tgtcatgcag
     3541 cacctgcggc gcggccgaat cttctttggg ccaagtcacg aaagcgagac cctgaacctc
     3601 gtgaagacgc accgtcaata tcgtaaaacc tgcggcaatg cgccccgcca gataggcaat
     3661 ctcgactcct actgtaataa gagatcccca tcagcaatag atgcgtgaac caacacaccg
     3721 tcatggagta tggcgaacag tcgcgcaatc tcgtcagcac cgcggatcat cgtcgtgttg
     3781 cctcgtgcgg gtcgaagctt ctggtaagcc ggcgctggcc gtctgcggcg atttgctcga
     3841 aatggctatc gagcgggatg aaaacgtccc atcgagattt gccc
//
LOCUS       DASTKU010000956         2824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_409614, whole genome shotgun sequence.
ACCESSION   DASTKU010000956 DASTKU010000000
VERSION     DASTKU010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409614"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VFQ61_33910"
     CDS             complement(<1..595)
                     /locus_tag="VFQ61_33910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959553.1"
                     /translation="MTDGRSLPDGRALPPNEEAAIQRERDPNQRRAVRFGALLGGLAL
                     GLLLVSWFVRSRAVSLAVFGSGSLAAFALFQLATGESRRAHRERPHTAGRIVESRQGT
                     AHVLFLLPGLSLEPWRADAWVLWVYSGMSAWAGNSWIGRAAGFVQVVLVGLLALRVVA
                     SARARFRIELEPGRWLVEAVVAGRSLQRTGRGALMPEL"
     gene            complement(592..1698)
                     /locus_tag="VFQ61_33915"
     CDS             complement(592..1698)
                     /locus_tag="VFQ61_33915"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET9959554.1"
                     /translation="MAALFRATHGKLGLVQPVLGPPATKGHVSVARLTRYWYVACQSH
                     ELGSRPVARVLLGTPFVLFRDAGGKAAALLDRCPHRNVPLSLGRVNGRGHLECPYHGW
                     QFDTEGKCRAIPGLGDAAAARERSCPRAAVCEQDGLVWVFPALDEAPASEPPDLGFAK
                     LTDHTWVRQEVRAQATLHATLENALDVPHTAFLHRGLFRGGERHAVTVRVRRSTDRVE
                     AEYVGEPRPSGVVGRLLSPTGGVVEHWDRFLLPSIAQVEYRLGPESHFLITAFCTPES
                     DFSTRLIAVAGFKTPLPGRILAPLLKPVALLIFNQDARILRAQSKNVQSFGGERFEST
                     ELDFLGPHIWRLLKQAERGELGEASAEREVTFFA"
     gene            1902..>2824
                     /locus_tag="VFQ61_33920"
     CDS             1902..>2824
                     /locus_tag="VFQ61_33920"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9959555.1"
                     /translation="MAEKPAVDKDTASFMRSLCMGQIAEDIVLPFPRIPETERETLKG
                     VIGSIEQLLGPREQDFKAWDKQGEFPPAFIDELRQFGLFGLVIPEEHGGLGFGSAAYS
                     RTLQEVASYDASVAVTIGAHSSIGMRGLLLFGTEEQKRRYYDKLASGEMIAAFCLTEP
                     TSGSDAASIKTTAVRDGNDWVLNGNKLWITNGGIADFFTVFAKTGDKDQRGHLSAFIV
                     TREMAGVSHGPHEDKMGIRASSTTTVHFDNVRVPAENLLGEEGKGFKVAMRILNSGRT
                     GLGGGSVGGMKRLIRLAVAQAKNRVQFGKPIA"
BASE COUNT          581 a          955 c          846 g          442 t
ORIGIN      
        1 tgagctctgg catcaaagcg cctcggccgg ttcgctgcaa ggaacgcccg gccaccactg
       61 cttcgacgag ccaacggccc ggttcgagct cgatccgaaa ccgcgcgcga gcggaggcca
      121 cgacgcgaag cgcgagcaat cccactaaca cgacttgcac gaagcccgcc gctctcccaa
      181 tccagctgtt tcccgcccaa gcgctcatcc ccgagtagac ccacagcacc caggcgtccg
      241 ctcgccacgg ctcgaggctc aagcctggca gcaagaagag cacatgagcc gtcccttgcc
      301 tggattcgac gatccgcccc gcggtgtgcg gccgttcgcg gtgcgcgcgc cggctctctc
      361 ccgtggccag ctggaacaag gcgaaggcag caagcgagcc acttccaaag accgctagcg
      421 ataccgctcg agagcgcacg aaccacgaga ccagcagcaa ccccagcgcg agccctccga
      481 gcagcgctcc gaaccgcacc gcgcggcgct gattggggtc gcgctcgcgt tggattgcag
      541 cctcctcgtt gggaggcagc gcgcgaccgt ctgggaggga gcggccgtca gtcacgcgaa
      601 aaacgttacc tcacgctccg cgctggcttc gccgagctca ccccgctccg cctgcttgag
      661 taaccgccaa atgtgcggtc ccaaaaagtc gagctcggtc gactcgaaac gctcgccccc
      721 gaaggactgc acgttcttgg actgcgcacg caaaatgcga gcatcctggt tgaaaatcaa
      781 aagcgcgacc ggcttgagta acggcgccaa gatgcgcccc ggcagaggcg tcttgaaacc
      841 cgcgaccgcg atcaatcgcg tagagaagtc actctctggc gtgcagaacg ccgtaatcaa
      901 gaaatgggac tcgggcccca gccgatattc gacctgcgca atcgacggga ggagaaagcg
      961 atcccaatgt tccaccacac cgcccgtcgg cgacaacagc ctgcccacca cgccactcgg
     1021 tcgcggctct cctacatatt cggcttcgac tcgatcagtc gagcgacgca cccgcaccgt
     1081 gacggcgtgt cgctcacccc ctcgaaatag gcctcgatgc aggaacgccg tatgcggcac
     1141 gtccagggcg ttctccaaag tcgcgtgcaa cgtggcctgc gcccgcactt cttggcgaac
     1201 ccacgtgtgg tccgtcaact tcgcgaagcc caagtccggc ggttcgctcg ccggagcttc
     1261 atcgagcgcc gggaagaccc acaccaaccc atcttgctcg cagaccgcag cgcggggaca
     1321 cgagcgctcc cgagccgccg ccgcatctcc tagccccgga atggcgcgac acttaccctc
     1381 ggtatcgaat tgccagccat ggtaaggaca ctccaggtgt cccctgccgt tcacccgacc
     1441 gagagacaac ggcacattcc ggtgcgggca gcgatccagc agcgctgccg ctttaccgcc
     1501 cgcgtcacga aacagcacga aaggtgtccc gagtaggact cgcgccaccg gtcgcgaacc
     1561 aagctcatga ctctggcatg ccacgtacca atagcgcgtg aggcgcgcca ccgaaacgtg
     1621 acccttggtt gcgggcggac cgagcaccgg ctgcacgagc ccgagcttgc catgggtcgc
     1681 gcggaatagc gctgccaatc gacgcctcga agttgaaagc ggaccgagca aggccgtgcc
     1741 cgtcgacccg acgatcgtgg cccggaccgt tgtcccaggc cttgccagcc tcgccgcggg
     1801 cccgcatagt tttggaagcg agctcaccgc caccactcgc cattccctgc agtatttcca
     1861 ctgcagcatt gcttcccgca ttgccttcca aggaggaccc agtggccgaa aaacctgcgg
     1921 ttgataaaga cactgcaagc ttcatgcggt cgctgtgcat gggccagatc gccgaggaca
     1981 tcgtattgcc cttcccccgc attcccgaga cggagcggga gaccctaaag ggggtaatcg
     2041 ggtcgatcga gcagctgctc gggccgcggg aacaagactt caaagcctgg gacaagcaag
     2101 gtgagttccc gcctgccttc atcgatgaac tgcggcagtt cgggcttttt ggattggtga
     2161 ttcccgagga gcatggtggg ctcggattcg ggagcgccgc gtactcccgg accttgcaag
     2221 aagtcgccag ctacgacgcc tcggtggcag tcacgattgg ggctcacagc tcgattggca
     2281 tgcgcggatt actgctgttc ggaacggagg agcagaaacg ccgctactac gacaagctcg
     2341 cgagcggtga aatgatagcc gccttctgcc tcaccgagcc aacgagtggc tcggacgcgg
     2401 catccatcaa gacgaccgca gtacgagacg gcaatgactg ggtgctgaac ggcaacaagc
     2461 tgtggatcac caacggcggc atcgctgatt tcttcacggt ctttgccaag accggagata
     2521 aggaccaacg cggccacttg agcgccttca tcgtgacgcg cgagatggcg ggcgtgagcc
     2581 acgggccgca cgaagacaag atggggatcc gcgccagctc gaccaccacc gttcacttcg
     2641 acaacgtgcg cgtgccggcc gaaaatctgc tcggcgaaga aggcaagggc ttcaaggtcg
     2701 cgatgaggat cttgaactcg ggtcgcaccg gcttgggcgg tggctcggtg ggcggcatga
     2761 agcgcttgat tcggctcgcc gtggcccaag ccaaaaatcg agtgcagttc ggcaaaccca
     2821 tcgc
//
LOCUS       DASTKU010000957         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1306038, whole genome shotgun sequence.
ACCESSION   DASTKU010000957 DASTKU010000000
VERSION     DASTKU010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306038"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFQ61_33925"
     CDS             <1..240
                     /locus_tag="VFQ61_33925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959556.1"
                     /translation="AEHGEGQSDYDDESNPVALHGTLLDEPIRELDPSSPSVPGPGPA
                     PFRIVATYGTEVGKYIVERYCSTLSQRRISPRPGR"
     gene            370..882
                     /locus_tag="VFQ61_33930"
     CDS             370..882
                     /locus_tag="VFQ61_33930"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HET9959557.1"
                     /translation="MPGLTRSLLKLLAFGGLSALTAAAGALVNAKPQNRIWYRALRKS
                     KLTPPDRVFGIVWPALYTLSAVSGWRVANTPSSRDRTLALALWGTQLALNAAWTPLFF
                     GAHRPRLAFMDLAANAAALAGYTHVSGRFDRNASRLMWPYLGWLGFAAILNGAVVVKN
                     RGTTRWLARR"
     gene            1214..2464
                     /locus_tag="VFQ61_33935"
     CDS             1214..2464
                     /locus_tag="VFQ61_33935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9959558.1"
                     /translation="MTKASVVVSFAFAMAACGGGNSSSNSDNNTPINLGSGASGNGSG
                     AAGGGTPGSGAVNQTTVDQLRAQACQGWSSEPESLPSILEFVVDTSGSMNDMTASTRG
                     QTKWNRTRDALTQSLDELPASVGVGLLLFPNMNTLASDQPADVTNCVNTRALIPLALL
                     GPAGSAQRRTLATSINAVMPQSYTPTYDAYTSGVDLGLLPSKLPGKRFMVLITDGAPT
                     LAAGCVMPGGGGRMRGGGGGMVTPVDPTPIVQAIQLAHDHGIQTFIIGSPGSEESING
                     TDARVAWLSKAARAGGTDTAGCSDAGPNFCHIDLTQSQDFAAALEGSLAKIAGQVVQC
                     SYPLPSAPSGKTLDLNAINVVYTSGNGSQSLLSRSADANCTEGWKLTGSQVDLCSNTC
                     NTVKADSAAKLELLFGCNSVTAVK"
BASE COUNT          479 a          810 c          797 g          446 t
ORIGIN      
        1 gccgagcacg gcgaaggaca gtccgactac gacgatgaaa gcaatccagt agcgcttcat
       61 ggcactctcc tcgacgagcc gatccgcgag ctcgacccaa gctcaccgag cgtccctggc
      121 cctggcccgg ctccgtttcg gatcgtagcc acgtacggaa cagaggtggg gaaatacatc
      181 gtcgaacgat attgttctac cctctcgcag aggcgcattt cgccaaggcc aggccgatga
      241 gctccgtgct cgcgcgtgcg gagctggcgc atcccttgct acgccggagg aatgcagctc
      301 gaatcggtcc ctccgtcgtc ccgcggcttc cgccaactca cctcagacga ggcattgtcg
      361 caagcaagcg tgcctggcct cactcgctcg ctgctgaagc tgctcgcctt tggcggcctc
      421 tccgcgttga cggcagcggc gggagcgctc gtcaacgcga agccccaaaa tcgcatttgg
      481 tatcgagcgt tacggaagtc gaagctcaca ccgcccgatc gcgttttcgg catcgtctgg
      541 cccgcgcttt acacgctgtc ggctgtctcc ggctggcgcg tcgcgaacac gccgagctcg
      601 cgcgatcgca cgctggcgct cgccctttgg ggcacgcagc tggcgctgaa cgcggcctgg
      661 actccgttgt tcttcggggc acaccggcca cgcctcgcat tcatggatct cgcggcaaac
      721 gctgcggcgc tcgcgggcta cacccatgtg agcggacgct tcgaccgcaa cgcttcacgg
      781 ctaatgtggc cttatctcgg ctggctcggc ttcgccgcaa tcctcaacgg cgccgtcgtc
      841 gtgaagaacc gagggaccac gcgctggctc gcgcggcgat gacgcccggc aaacgtcgcg
      901 atggcgatcg aaaacgacga gcacgacttc gtcacgagcc gcccgggcgg ttaagtcccc
      961 attaagctgc ggggggccga aacagccggg cccctcgagc acagccggct ccgtggagca
     1021 gcgcctacag cagaccacgt cggcgaaggc tcgtgccgaa tttctgcctg caaaggcggg
     1081 gtacacggca ctaagcacgt gagcgcaccg tccggtaaaa gaaagatttc ttcgttagtc
     1141 gctgcgcgca gggcgatatc ggctgaagtg tcgcaagcgc gtggttcttt cgagaaaagc
     1201 aggtaactcg ctgatgacaa aagctagcgt cgtcgtttcg ttcgcgtttg ccatggccgc
     1261 ctgtggaggc ggcaactctt cgtcgaactc ggacaacaac acgccgatca atctcggaag
     1321 tggtgcgtcc ggtaacggga gcggggcagc gggcggtgga acgccgggca gcggcgcggt
     1381 gaaccagacg acggtggatc aactgcgagc tcaagcctgt cagggctgga gcagcgagcc
     1441 agagtcgctg ccctctatcc tggaattcgt cgtagatacg agcggctcga tgaacgacat
     1501 gacggcttcg acgcgcggcc agacgaagtg gaaccgcacg cgcgatgcat tgacccaatc
     1561 gctcgacgaa ttaccggcgt cggtcggtgt cggactcttg ctatttccca acatgaatac
     1621 gcttgcgagc gatcagcccg cggacgtgac caactgcgtg aacacgaggg ctctcattcc
     1681 actcgctcta cttgggcccg ccggctctgc tcagcgccga acgctcgcga cgagtatcaa
     1741 cgcggtgatg ccccagtcgt acacgcctac gtacgacgct tacacgtccg gagtcgatct
     1801 cgggctgctc ccgtccaagc tccccgggaa gcgattcatg gtgctcatca ccgatggcgc
     1861 cccaacgctt gccgctggtt gtgtcatgcc cggcggaggc gggcgcatgc gaggcggagg
     1921 cggtggcatg gtcacaccag tcgatccgac gccaatcgtg caggcgattc agttggcgca
     1981 cgaccacggg atacaaacct tcatcatcgg ctcgccaggg agcgaagagt cgatcaatgg
     2041 aaccgacgca cgggtcgcgt ggttgtccaa ggccgcgcgc gccggtggca ccgacaccgc
     2101 cggttgctcg gatgcaggcc ccaatttctg ccacatcgat ttgactcaaa gccaagactt
     2161 cgcggccgcg ctggagggaa gcctcgcgaa gattgcaggt caagtggtgc agtgctcgta
     2221 tccgctaccg agcgcgccga gcggaaaaac cctcgacctg aacgccatca atgtcgtcta
     2281 cacgtccggg aatggcagcc agtcgttgct ctctcgcagc gctgatgcca attgcaccga
     2341 gggttggaag ctcacgggct ctcaggtcga tctctgcagc aacacttgta acacggtgaa
     2401 agcggactcg gctgcgaagc tcgagctgct gttcggctgc aacagcgtga cggcggtaaa
     2461 gtagccgcgg ctcgaccggg gccgtgggct gtaaaatagc cgcgacgcga gcggggcggt
     2521 gtgttcttcc gt
//
LOCUS       DASTKU010000958         8860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_633893, whole genome shotgun sequence.
ACCESSION   DASTKU010000958 DASTKU010000000
VERSION     DASTKU010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8860
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633893"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="VFQ61_33940"
     CDS             complement(<1..1537)
                     /locus_tag="VFQ61_33940"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HET9959559.1"
                     /translation="MTETQISSPQTSSSARTRARLKLQARLKRAWPALAVLILGPALG
                     FAWSEHAAHGLPVHARMLGKDVVALRGDLASLYAAARERERVRIELHSSTATAALTAQ
                     QAGLSIDVDETLRVARRDYAASSIALRFARWARGLFDPLPEVRLVVREDAQRLDLALS
                     GVEKTVAMAPYAGGFRVVGRDIVPDYPKPGSRLDRGQVRSALLHALATQQTRIAARTQ
                     SVPAPLPNAAIDRLVGAAKAATREAVRLLEVGSGRSVEVTREVLLAALRVNPPSDPAD
                     APELAFDPEVLKRELSTVWATLEHEPVAARFTIDAQDHVHIVPGITGARLDLGLLGEA
                     TLAAASNPERVARLPLSCDREAEPSTAALRDLGVRGLVSSFTTRHPCCEARVANIHRI
                     ADLLDGVIVKPGETFSVNALVGPRTQKNGFVPAPTIEEGEMVETLGGGISQFATTLFN
                     AVFHGGYEIIERQPHSYWFPRYPMGHEATLSYPKPDLIFRNDTQAGVLIDTQYTDKSI
                     TVRL"
     gene            1675..2256
                     /locus_tag="VFQ61_33945"
     CDS             1675..2256
                     /locus_tag="VFQ61_33945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959560.1"
                     /translation="MWVRRSAIRPVPSYASVPPQVLEAVRDELADDDEEARAQLDDAF
                     ERFERAQPALAAYVAETLGRPLDETALALGYFLALTVWLAFDRAHGSHMNEVTEDELV
                     ATEELLTLDEELRRADPAESLDSDDVIAMEQPHVVAFVHEHIDGTLDAHAEEIDVNDV
                     HTVYRVVLVELLALSYCVRRPDGFPVSKTELLA"
     gene            complement(2273..3778)
                     /locus_tag="VFQ61_33950"
     CDS             complement(2273..3778)
                     /locus_tag="VFQ61_33950"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9959561.1"
                     /translation="MAGKDAPLSVIALQIVKEALRPEATPAALAQMAERDPAFALRVI
                     AMVNSAAFGAARRVSDVKQAAALLGVRGLRSVGMSLALSDMIPVSGEAEVLLANSLRR
                     AVAAQLLADRFGERDRNEYFTAGLFLELGILARAREDLKGAAQVARAPAADRPLFERA
                     YGVREHPIRGAELAVDFHLSEATIAAIRGHHDAAPPETVVGKVTWLAERIAGVWEGGD
                     VAQTRGLALEGCRSVGLSEADLDDVLRSLPEHVTQASSAFDRKIDSQKSYEALLVDVN
                     RGLLELTNSYEAVVRQLEVLLEEKEDLTRRLAEANDRLERLAHTDALTGLANKRALEQ
                     ALARDLARADRDRTSVCVIVADVDHFKKVNDRYGHPTGDVVLKGVAKALSANLRSGDL
                     ATRFGGEEFVLILPGSNTFGGQLAAERIRKSIEARRFDTADGPLQVTMSFGVASVCGP
                     GCGGGAADLIAAADSALYQAKQQGRNRVMSYTPAGTSANEGSPKPAASVPK"
     gene            complement(3922..4500)
                     /locus_tag="VFQ61_33955"
     CDS             complement(3922..4500)
                     /locus_tag="VFQ61_33955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959562.1"
                     /translation="MEDARVPFGRDVYLALASVGWERGMLQPHHADAIVRLALDECLD
                     FEEVLEIEEAIRHPVALAMLEAARLSKSDRLFLYAVGVWIAGMGDHRGAFEERAALGE
                     GALARLGTALRVDSRAQRIVEGIVAKFTESCEQRSGGRSSRLDTKLLRRTILERVSAA
                     EQRRASGIQPRQSAAVALEAESRHGLASEYRR"
     gene            4735..4986
                     /gene="moaD"
                     /locus_tag="VFQ61_33960"
     CDS             4735..4986
                     /gene="moaD"
                     /locus_tag="VFQ61_33960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HET9959563.1"
                     /translation="MKVRILYFAKARDLAGTSEETVELRPEVVTLRDLARELIELHPA
                     LGAGALERMRIARNESFAELDERPTDGDVIALIPPVAGG"
     gene            5001..5984
                     /locus_tag="VFQ61_33965"
     CDS             5001..5984
                     /locus_tag="VFQ61_33965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959564.1"
                     /translation="MAAFSYLVGVDENGLGARLGPLVVTGVLAKADDLGRRALSRKLP
                     ASVRADLDDSKRLVSHSDVALGEAWARAVLPEQIETPDQLLEHLLYQGQASLSAICPD
                     HVRPQCWGTTSEAFCAEPALLKRVQKHLTWFRERGVEVRRAMTHVQCTKRLNDARAGG
                     RNRFVADLHAMEDLLLRLRAEATGNVLAVCGKVGGIGSYGKFFGPLSNHLHAVIEEGK
                     ALSAYHFPKLGEVRFLRDADAEEPLVMLASLIGKYVRELLMARIARFYPEPGAPAPSG
                     YHDPISHAFVLRTALLRKSQSVPDTCFERERDEAADRPGARERGEQGAAEV"
     gene            5981..7483
                     /locus_tag="VFQ61_33970"
     CDS             5981..7483
                     /locus_tag="VFQ61_33970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET9959565.1"
                     /translation="MTASNAHPANTGPDVGQSPSLTPASERILSGRLGLEMARFGTPL
                     AIGMGLQTAFNLVDAYLIGQLEPDVASPALGAIGICDQVTALGTILSYGLTVAVGALM
                     SHFQGRRDPVAVRRVLYQSLWLVLGLSAMFALVGLVGSRWLLSSAVGTKGEVLALGIP
                     YLSVMMTTSSGIFLMLHFTSSLRAVGSSKTPVSLLVLANVVNLLLAIVLVYGPGPAPA
                     IFSWGPPLARLLGLPRMGLMGAAWATAIARFATLIPLVIILIRRFGLPESWSELRPDR
                     ALLRRILTVGWPSSVQLVVRILAMLFTHALVARAFTSPGDQSATTALGIVFRLETMAL
                     FVGLGWGSAAQTFVGQNLGADKLLRAKSSGWYAAAFNALMMAGLWLLYTRFGELIIGM
                     FDTNPSVVNVALDYLRWVSQSYIALGIGIVLGSAIQGAGATRKALWLDGSVVLLFQMP
                     ISLLAVLAPGASTVRLFQAVALTYLAFALVHVASYWRGRFLHNSTLHVNA"
     gene            complement(7535..8683)
                     /locus_tag="VFQ61_33975"
     CDS             complement(7535..8683)
                     /locus_tag="VFQ61_33975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959566.1"
                     /translation="MLRKTQIGKTLIGMLCMALGVSSLALACSSGSETDAGAMGGATN
                     AGNSTGAKTNGGAANRGGGTGSGAEAGESNGEAGVGNEGNAAGSANQGGSVSRGGAGN
                     GGAGNGGSAGTLVNGGATSGGSSTTSTGLSACDNGKDDDGDGLVDGLDPECTGPLDNQ
                     EDSFATGIPGDNRDPKWQDCFFDGNSGAGDDQCRYATGCLTGDLPADHADCTVSQACL
                     DFCAPLTPNGCDCFGCCTIALDDGSTVDVLENSACSLANLDDEKACPRCVKSEQCGNN
                     CGECELCPGKTAADLPASCKPPTSGTGGASGVGGAPSMGGATSSGGAANGGTSSRGGA
                     SNSGGATGTSPPPPSYTCDNGEQVCGAGMPGCETGYYCSLGCCLLSIR"
BASE COUNT         1510 a         2915 c         2768 g         1667 t
ORIGIN      
        1 acagccgaac cgtgatgctc ttgtccgtgt attgggtgtc gatcaagacc cccgcctgag
       61 tgtcgttgcg aaagattaga tcgggcttgg ggtagctcag ggtagcttca tgacccatcg
      121 ggtagcgcgg aaaccaatag ctgtgcggtt gccgctcgat gatctcgtat ccgccgtgga
      181 agacggcgtt gaacagcgtg gtcgcgaatt ggctgatccc gccgccaagg gtctccacca
      241 tctcgccttc ttcaatcgtg ggagcgggga cgaagccatt cttctgcgtc ctcgggccca
      301 ccagcgcatt gaccgaaaag gtctcccctg gctttacgat cacgccgtcc aacagatccg
      361 caattcggtg gatgtttgcc acgcgcgctt cacagcaagg gtgtcgcgtg gtgaaagagc
      421 tgacgagacc gcgcaccccg aggtcccgca gcgcagcggt gctgggctcc gcttcgcgat
      481 cgcacgacaa gggcagccgt gcgacccgct ccgggttcga cgccgctgcc aacgtggctt
      541 ccccgagcaa gcccagatcc agccgagcgc cggtgatgcc cgggacgatg tgcacgtgat
      601 cctgggcatc gatcgtgaag cgagccgcga cgggttcgtg ttcgagcgtg gcccagacgg
      661 tcgagagctc gcgcttcaga acttcgggat cgaaagccag ctccggcgcg tccgcaggat
      721 ccgacggcgg gttcacgcgc agcgctgcca gcaacacctc gcgcgtgacc tcgacgctgc
      781 gccccgatcc gacctccagg agcctcactg cctctcgagt ggctgcctta gctgccccta
      841 ccaggcgatc tatcgctgcg tttggaagcg gcgcaggaac gctctgggtt cgagcggcga
      901 ttcgcgtctg ctgcgtggct agcgcatgca gcagagcgct ccgtacctgc cctcggtcga
      961 gccgacttcc gggttttggg tagtcgggca cgatgtcgcg ccccacgacc cgaaagcccc
     1021 cagcgtaggg agccatggcc acggtttttt cgacacccga caacgccaaa tccaagcgct
     1081 gcgcgtcttc gcgcacgacc agccgcacct ccggcaaagg gtcaaagagg ccgcgagccc
     1141 aacgggcgaa ccgtagagcg atcgagctcg cggcatagtc gcgacgcgcg acgcgcagcg
     1201 tttcgtcgac gtcgatcgat agccctgcct gctgcgcggt gagcgctgcc gtggccgtgg
     1261 aactatgcag ctcgatgcgg acccgctcgc gctctcgcgc ggcagcatag agcgacgcga
     1321 ggtctccgcg tagtgcgacc acgtctttgc ccaacatccg cgcgtgaacg ggcagaccat
     1381 gcgcggcgtg ttcgctccat gcaaagccga gcgccggccc gaggatgagt accgctagcg
     1441 cgggccaggc gcgcttcaga cgcgcttgca gcttcagacg cgctcgcgta cgcgcactgc
     1501 tgctcgtttg gggcgaggag atctgggttt cggtcacgcg cgccctccta tggatcagat
     1561 cggacgcaga ctgaagagtc cgattggccg aggcacctgc gacacgcacc gacctgcgag
     1621 aggccaggat ttcgacccca cgaagaaagg gccgtgctac atgtcacgag tctcgtgtgg
     1681 gtccgtcgtt cggccattcg cccagttcct tcctacgctt cggttccccc gcaagtgttg
     1741 gaagccgttc gtgacgagct ggcggacgac gacgaagaag cccgcgcgca gttggatgac
     1801 gctttcgagc gtttcgaacg tgcgcagcct gcattggccg cttacgtcgc cgagacgctc
     1861 ggtcggcctt tggacgagac ggcgctggct ctcggctatt ttctcgcgct caccgtgtgg
     1921 ctcgctttcg atcgcgcgca cggctcgcac atgaacgagg tcacggaaga cgagctggtg
     1981 gcgaccgaag agctcctcac cttggacgag gagctgaggc gggccgatcc ggcggagtcg
     2041 ctggatagcg atgacgtgat cgccatggaa cagccgcacg tggtggcctt cgttcacgag
     2101 cacatcgacg gcactctcga cgcccacgcc gaagagatcg acgtgaacga cgtgcacacg
     2161 gtctaccgcg tagtgctggt agagttgctg gcgctctcgt actgcgtgcg ccgtccggac
     2221 ggtttcccgg tatccaagac cgagctgctt gcttagagcc ttgccggctg cgttacttgg
     2281 ggaccgaagc ggcgggtttc ggtgaacctt cgttagcgct cgtcccggcg ggggtatagc
     2341 tcatgacgcg atttcgcccc tgctgcttgg cttgatacag agcgctatcc gcggccgcaa
     2401 tcaagtccgc tgccccccca ccacagcccg ggccgcaaac gctggcgacc ccgaagctca
     2461 tcgtgacctg cagcggcccg tctgcggtat cgaagcgtcg cgcctcgatc gacttgcgga
     2521 tgcgctcggc cgcgagctgc ccgccgaagg tgttggagcc cggcagaatc aacacgaact
     2581 cttcgccgcc gaatcgcgtc gccaaatctc cactgcgcaa gttcgctgat aaagccttcg
     2641 cgacaccctt caacaccacg tcacccgtgg gatggccgta acgatcgttc acctttttga
     2701 aatggtccac gtccgcgacg atcacgcaaa cgctagtgcg gtcgcgatcc gcacgcgcta
     2761 ggtcgcgcgc gagcgcctgc tcgagcgcgc gcttattggc caagccggtg agcgcgtcgg
     2821 tgtgcgccag ccgctccaag cgatcattcg cttccgccag gcggcgcgtg agatcttcct
     2881 tttcctcgag cagcacctcg agctgtcgaa ccaccgcttc gtagctattc gtgagctcga
     2941 gcaaaccgcg gttgacgtcc acgagcagcg cctcgtagct cttctgtgaa tcgatcttgc
     3001 gatcgaaggc gctgctcgct tgggtgacgt gctcgggcag actgcgcagc acgtcatcca
     3061 gatcggcttc gctaagcccc acgctgcggc agccctcgag cgcgagccct cgagtttgag
     3121 ccacatcccc gccctcccag acccccgcga tccgctctgc gagccacgtg accttgccca
     3181 ccaccgtctc gggtggagcg gcatcgtggt gaccacgaat ggcggcgatg gtcgcctccg
     3241 aaagatgaaa atccaccgcc agctcggcgc ctcgaatcgg gtgttcgcgc acgccgtaag
     3301 ctcgttcgaa cagcggacga tcggccgcgg gtgcacgcgc cacctgcgct gcacctttca
     3361 aatcttcgcg agctctcgcc aaaatgccga gctccaagaa caggcccgcg gtgaagtatt
     3421 cgttgcgatc gcgctcgccg aagcggtcgg cgagcagctg cgccgcgacc gcacgtcgca
     3481 gcgagttcgc gagtagcact tcggcttctc cgctcaccgg gatcatgtct gacaacgcca
     3541 agctcatgcc gacgctgcgc aaacctcgta cccccagcag cgccgcggct tgcttcacgt
     3601 cactcactcg cctggccgcc ccgaaggccg cgctattgac catggcgatc acgcgcaaag
     3661 cgaacgcagg gtcccgctct gccatctggg cgagggcggc gggtgtggcc tcaggtcgta
     3721 gggcttcttt gacgatctga agcgcaatga cggagagagg ggcgtctttg ccagccattc
     3781 gagcggtaac gcgcctttcg tggcaacccg tgaaacgggc gaaatgcgct caattaacgc
     3841 caacggccgt gacgagattg taataacgag tcagccgata ctgcgtaaat gcgcaggctg
     3901 agcctcacgc catgagctgg ctcagcggcg atactccgac gccagaccgt gccgagattc
     3961 cgcttcgagt gctacggcgg cggactgtcg cggctgaatc cccgacgcac ggcgttgctc
     4021 cgccgcgctc acgcgttcga ggatggtccg acgcaagagc ttcgtgtcca agcgggatga
     4081 ccgaccaccc gacctttgct cgcagctttc cgtgaacttc gcgacgatac cttcgacaat
     4141 ccgctgggcg cgcgagtcca cacgcagcgc agtccccagc cgcgcgagcg caccctctcc
     4201 tagggctgct ctttcttcga acgcaccgcg atggtcgccc attccggcaa tccagacccc
     4261 aaccgcgtac aggaaaagcc tgtcgctttt cgataggcgt gccgcttcga gcatagccaa
     4321 tgcaactgga tgtcgaatgg cttcctcgat ctcgagtact tcctcgaaat ccaagcactc
     4381 atcgagcgcc agccggacaa tcgcatcggc gtgatgcggc tgcagcatac cgcgctccca
     4441 cccgaccgag gccaaggcca ggtagacatc tcttccgaac ggaactctcg cgtcctccat
     4501 aggtcgcttt cgagcggctg aacgaacaca gccggcgcga actacgctac tcgcgtcgag
     4561 gtctcgacgg aagtagtttc ttgtgggcgc ctcgactcca gcggcagggg tgacgagctt
     4621 gcccgcattg accaaactcg gcgctcattc gtctggtttt tgagttccag gctcgcaggg
     4681 ccggtgcacg ccgcgagttg aaacctgctg ccaattgcgt accctgcggc atccatgaag
     4741 gtgcgcatcc tctatttcgc gaaagcgcgc gacctggctg gcacctcgga agaaaccgtg
     4801 gagctccggc ccgaagtcgt caccctgcga gacctagctc gtgagctgat tgagctgcat
     4861 ccggcgctcg gggccggggc cctagagcgc atgcgcatcg ctcggaacga gtcgtttgcc
     4921 gagctcgacg agcggccaac ggatggagac gtgattgcgc tcatccctcc cgtcgcgggc
     4981 ggctagtacg aggtcgagca gtggcggcgt tcagttacct agtgggtgtc gatgaaaacg
     5041 ggctcggggc gcgcttgggt ccgctcgtgg tcacgggcgt tctcgccaaa gccgacgatc
     5101 tcggccgacg agcattgtcg cgtaaattgc ccgcctcggt gcgagccgat ctcgacgact
     5161 ccaagcggct cgtctcgcat tcggacgtgg cgctcgggga agcctgggca cgcgccgtgc
     5221 ttccagagca gatcgaaacc cccgatcagc tgctggagca tctcctgtat caaggccaag
     5281 catcattgtc ggcgatctgt cctgatcacg tgcgcccgca gtgctggggc acgaccagcg
     5341 aagcattctg cgccgagccc gcgctgctca agcgcgttca gaagcacctg acgtggtttc
     5401 gagagcgcgg cgtagaagtg cgccgcgcca tgacccacgt tcaatgcacg aagcgcctca
     5461 atgacgcgcg agcgggtggg cgcaaccgat tcgtcgcgga tctacatgcc atggaagatc
     5521 tgctgcttcg gttgcgcgcc gaggcgaccg gcaatgtgct cgcggtttgc gggaaggtcg
     5581 gcggcatcgg cagctacggc aagttttttg ggccgctgag caatcacttg catgcggtaa
     5641 tcgaagaggg aaaggcgctt agcgcgtatc acttcccgaa gctcggggag gtccgctttt
     5701 tgcgagatgc agacgcggag gagccgctgg tgatgctcgc ctcgctgatt ggcaagtacg
     5761 tgcgtgagct cttgatggcc cgcatcgcgc gcttctatcc tgaacccggc gcgcccgctc
     5821 cgagcggcta tcacgacccc atcagtcacg ccttcgtctt gcgaacggcg ctgctgcgca
     5881 aaagccagag cgtgcccgac acgtgtttcg agcgcgaacg ggacgaagcg gcagatcggc
     5941 ccggagcgcg agagcgaggc gagcaaggtg cggccgaagt atgaccgcca gcaacgcgca
     6001 tccagccaac acgggccctg acgttgggca aagcccaagc ctcaccccgg cgtcggaacg
     6061 catcttgtcg ggtcggctcg ggttggaaat ggcgcgtttc gggacgccac tcgcgatcgg
     6121 catgggactg caaaccgcgt tcaacttggt ggacgcatac ttgattgggc aactcgagcc
     6181 cgacgtcgcg agcccggcac tcggcgcgat cggcatctgc gatcaggtga cggcgctcgg
     6241 aaccatcttg agctacggcc tgaccgtcgc ggtcggcgcg ctgatgagtc actttcaggg
     6301 ccggcgcgat cccgtggccg tgcggcgcgt gttgtatcaa tcgttgtggt tggtgctggg
     6361 cctatcggcc atgttcgcgc tggtaggctt ggttgggtcg cgctggttgc tgagtagcgc
     6421 ggtgggcacc aagggtgaag tgctggcgct cgggatccct tacttgtccg tgatgatgac
     6481 gaccagctcg ggcatttttc tgatgctgca cttcacgagc agcttgcgcg ccgtgggcag
     6541 cagcaaaacc cccgtaagcc tgctcgtgct cgccaacgta gtgaacttgc tgctggcaat
     6601 cgtgctcgtt tatgggccgg gccccgcacc ggccatattt tcctggggtc ccccgctcgc
     6661 gcggctgctt gggttgcccc gaatgggcct gatgggtgcg gcgtgggcca ccgccatcgc
     6721 gcgcttcgcg accttgatcc ccttggtcat catcctgata cgccgcttcg gcttgcccga
     6781 gtcctggtcg gagctgcggc ctgaccgtgc actgctacgc cgaatcctga ccgtcggttg
     6841 gccatcgagc gtccagctcg tggtgcgcat cctcgccatg ttgttcaccc acgcgctggt
     6901 cgcgcgcgcg ttcacgagcc cgggcgatca gagtgccacg accgcgctcg ggatcgtgtt
     6961 tcgactcgag accatggccc tgttcgtggg cttgggctgg ggcagcgccg cccagacgtt
     7021 cgtgggccag aatctcggcg ccgacaagtt gctgcgcgcg aagagcagcg gctggtacgc
     7081 ggccgcattc aacgcgctga tgatggcggg gctgtggctg ctctatacgc gtttcggcga
     7141 gctcatcatc ggtatgttcg acaccaatcc aagcgtggtg aacgtggcct tggactacct
     7201 gcgatgggta tcgcagagct acatcgcgct cggcatcggc atcgtgctgg gctcagccat
     7261 tcaaggcgca ggcgcaacgc gcaaggcttt gtggctggac ggctcggtgg tgttgttgtt
     7321 ccagatgccc atcagtttgc tggccgtgct cgcgcctggc gcgagcacgg tgcgtctgtt
     7381 ccaagccgtc gctctcactt acttggcgtt cgcgctcgtg cacgtcgcca gctactggcg
     7441 gggacgcttc cttcataaca gcacgctgca cgtgaacgcc tgattcacgc gcagcgcgcc
     7501 ctcgatggcc agaaatgctc caggacttcg agactcaacg gatcgaaagc aaacaacagc
     7561 cgagcgagca gtagtatccg gtttcacaac caggcatgcc cgccccgcaa acctgttcac
     7621 cgttgtcgca ggtatagctc ggaggcggtg ggctagtacc ggtcgctccg cccgaattcg
     7681 aggctccgcc ccggctactc gtaccaccgt tcgcggcgcc gccactacta gttgcgccgc
     7741 ccatactcgg agcgccaccc acacccgagg ccccaccggt gcccgaggtc ggcggcttgc
     7801 agctcgcagg caggtcagcc gcggtcttgc ccgggcaaag ctcacactct ccgcagttgt
     7861 tcccgcactg ctcactcttc acgcaacgcg ggcaggcctt ctcgtcatcg aggttagcca
     7921 gtgaacaagc cgagttctcg agcacgtcga cggtcgaacc atcgtccagc gcgatcgtgc
     7981 agcacccgaa gcaatcacaa ccgttgggag tgagtggagc acagaagtcc aagcacgctt
     8041 gggacaccgt gcaatctgcg tgatctgcgg gcaggtcacc cgtcaaacag ccggtcgcat
     8101 agcggcattg gtcgtcaccg gcgcccgaat tgccgtcgaa gaagcagtct tgccatttgg
     8161 gatcacggtt gtctcccggg atgcctgtcg caaacgagtc ttcctggttg tcgagcggcc
     8221 cggtgcactc cggatcgagg ccgtcgacga ggccgtcgcc gtcgtcgtct ttgccgttgt
     8281 cgcaggcgct gaggcccgtg ctcgtcgtgc tcgatccgcc gctcgtggcc ccgccgttga
     8341 ctagcgttcc cgcagagccg ccgttgccag cgccgccgtt gccagcgcca ccgcggctca
     8401 ccgaaccgcc ctgattggct gagccggccg cgttcccttc attgcccaca ccggcctcgc
     8461 cgttcgattc gccggcttcc gcgccgctgc ccgtaccacc accgcgattg gcagctccgc
     8521 cgttggtctt ggcacccgtc gagttcccgg cgttcgtggc gccgcccatc gcgccagcgt
     8581 ctgtctccga accggaagaa caggcgagcg ccaagctgct cacgcccagc gccatacaca
     8641 gcatgccaat cagcgttttt ccgatctgtg tttttcgcag catttttact cgacgtcggt
     8701 tgttcgttgt tttttcgctc gcgatgagct gggccaaggt caggggagca tttgcggtgt
     8761 cgaccactca gcggtcgaac tcgctctcgt tcaatgagtg gtgtcacccc tcgattcttt
     8821 tgaacggcgc aaagaaacgt gacgtgatcc ctcgcgacac
//
LOCUS       DASTKU010000959        11802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1306250, whole genome shotgun sequence.
ACCESSION   DASTKU010000959 DASTKU010000000
VERSION     DASTKU010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11802
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306250"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFQ61_33980"
     CDS             complement(<1..198)
                     /locus_tag="VFQ61_33980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959567.1"
                     /translation="MRLAKALENGLAASIGRRGQLALCCWQVLVTTALSGCIVTREKP
                     EPWPDTAEQSAYPVGWDARSSQ"
     gene            308..823
                     /locus_tag="VFQ61_33985"
     CDS             308..823
                     /locus_tag="VFQ61_33985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959568.1"
                     /translation="MHLRITLALGFLAFACSGGAQTSSPKAAEPATTQSAADPELSRA
                     EGELREAEQQLFGVLRAERETVAADESAPAPALPASPAPAPKAQAEEMKRAKPLEQPP
                     GESGGAAAAEGDGCQFGCRALSSMRRASDAICRITGDADPRCHSARERVRAAEQHLLR
                     SPCTCAKNEGE"
     gene            complement(847..2529)
                     /gene="ilvD"
                     /locus_tag="VFQ61_33990"
     CDS             complement(847..2529)
                     /gene="ilvD"
                     /locus_tag="VFQ61_33990"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET9959569.1"
                     /translation="MAQPLNKYSSRITQPKSQGASQAMLHATGLTRADLNKAQVGISA
                     VWYEGNSCNMHLNALAAKVKEGVEGAGLVGMRFNTIGVSDGISMGTDGMSYSLQSRDL
                     IADSIETVMSAQWYDANISIPGCDKNMPGCLIAMGRLNRPALMVYGGTIRAGKSEHCP
                     KLDVISAFQSYGELLAGRIDEERRLEIVEHACPGAGACGGMYTANTMASAIEAMGMAL
                     PYSSCTPAEDSGKLEECVRAGAAIKLLLERDIKPRDIMTREAFENAMVVIMALGGSTN
                     AVLHLIAMAKAVGVRLGLDDFQRVSDRIPFLADLKPSGKYVFEDLHSVGGIPAVMKYL
                     LDRGVLHGQCLTVTGRTLEENLRDLPGLAPDQEIIRPWDAPIKPTGHITILYGSLAPT
                     GSVAKITGKEGLVFEGDAIVFDCEEDLLASVERREVRKGHVVVIRYEGPKGGPGMPEM
                     LSPTSVIMGAGLGQDVALITDGRFSGGSHGFIIGHITPEAQEGGPIALVQNGDKIRID
                     STERRIDLLIPSEELARRQSQFVAPPLKVKSGALYKYVRLVKSASEGCVTDE"
     gene            complement(2699..3778)
                     /locus_tag="VFQ61_33995"
     CDS             complement(2699..3778)
                     /locus_tag="VFQ61_33995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959570.1"
                     /translation="MLSPFARSARGLLAAVSPLPVLCCNSLLDNPPGELATSAGSGSS
                     GGVHTAVPRTGGRSSSNGGRQVGTGGESSAGAAGEFEGGSGGNASSGGGATSHGGSGN
                     AGNAAGTSGGVNTSGGASTSGTATSSGGTSTLPQLPAQPVLWLMADRGVSVQGERVSK
                     VADQSGQGHDASQTNSGQMPRIVASASGPPLLEFDGADDALALPAGFKDFSGAGFFAV
                     VEALPNAGCAGILSFSNGDDRDDVEFGRHTPNLLYYEVLGSFVEGAKNAFEVDRRFLI
                     SIVQDNAGNVELRLNRTLTGSGKMLVPAAVSRAQNFVGKDVYDECPTPYHGRLGELIL
                     YARGVSGSERQELEDYLATRWGLQL"
     gene            3834..6194
                     /locus_tag="VFQ61_34000"
     CDS             3834..6194
                     /locus_tag="VFQ61_34000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HET9959571.1"
                     /translation="MSSEQPPIVAEPFDPIPVLAAELGLSPSSVAAVVRLMSEGATVP
                     FIARYRKEATGGLDEVQIRTLAERFEYVTELEARRNHVIEEIQKQGKLSPELLGKLRT
                     VTTKAELEDLYLPFKPKRRTRATIATERGLAPLAEQMWKQEGSGPLERAQRYVDPSKD
                     VPDAAAALAGARDICAERLAEDAELRKRLREEFLERGVIQVKKKAEFAEQVTKFDMYA
                     NFEEPIAKVPSHRYLAIRRGENEGVLYATLNLPSEPWIPFAKSRIPVRPGSAWAAELQ
                     LVVADALDRLLLPSCASEARVELKLRSDQEAIQVFARNLGELLLAAPLGGKSVLGIDP
                     GQRTGCKCAVVDGTGRLLEHTTLFLVQGPAALEKSKQTLLALCRTHEPDAIAVGNGTH
                     GRETEAFVREVLGEAGLGQVPCVSVSEAGASVYSASDLAREEFPELDLTVRGAISIAR
                     RLQDPLAELVKIDPKSIGVGQYQHDVTQSALGRKLDEVVESCVNSVGVELNTASAPLL
                     SRVSGIGPGLAKRIVAHRDTHGPFPSRKAMLKVSGLGPRTFEQAAGFLRVSGAEHPLD
                     ASAVHPERYALVERMAKDLGVALAALVGNSALVARLELDRYRSSDVGDFTLADIRAEL
                     DKPGRDPRKSFEPPQFRDDVRTLQDLVVGMDLQGVVTNITAFGAFVDIGVHQDGLVHI
                     SQLADRFVKDPHEIVKVGDRLRVRVLEVDITRKRISLTARSATPPQTASPRTAPAPVQ
                     KPAPKAAPNRSKQEARPAPTRSGADSTNAAQGFKHNPFARLLDKNK"
     gene            6287..6799
                     /locus_tag="VFQ61_34005"
     CDS             6287..6799
                     /locus_tag="VFQ61_34005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9959572.1"
                     /translation="MSPPHPPQPPLPLHVVLVDDDARQREQTQSWLESAGYRVSPCSG
                     LGDFERLVAATPPDLILIDVLMPAFGAADLERLLRHFPATSSSPVVLHSPLPAATLRR
                     LVKTSGALGILEATLTREAFVAAFRAFAETLLLRHRLRAANSGTHRIGSGSAEQNGAQ
                     GRDTRSRNSR"
     gene            complement(6838..9201)
                     /locus_tag="VFQ61_34010"
     CDS             complement(6838..9201)
                     /locus_tag="VFQ61_34010"
                     /inference="COORDINATES: protein motif:HMM:NF022812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HET9959573.1"
                     /translation="MIRRTTLRAACPFGLWLLLVACSSTSPDPHTGTGGSSGAGATSA
                     TGGSDSSGGRTQNTGGAQPIAGAASGGKASGGAGGQPVGGREDLNTDEKQELQRLQGM
                     LDANHGLTAEELLEKRALKRPEKLSYRPGDAEFLDRIQESALKLNASELAKLDENGFV
                     VSTRSAFPTFVRGYAAIYSEHLPVYVSADAILEAVHSSYEDLLILFESGALIPKLDTL
                     LSAIHERVAAGASANPKIAADLDLYMTVARSLLAGKTLAPLAGGDTKLISQIVKMATD
                     AQGEANLVLFGAPRIVDASQFKPRGHYDEDPTLSRYFRAMMWLGRMDFRLIETNPDGS
                     QTFLRPQYEAMLLLYQSMSEQSLADYELIDSALRTFVGDSDNMTVGQVNALVDGLGGA
                     EKAAQRSDVEIEAAIVQGGFGAQKIASQIMVNDGVVPTLPLARSFALFGQRYVIDSHV
                     FSQVVYDRLPAFRMMPNPLDAAYAALGNNQALSLLAPELSQFTGYPGALESARDLVDA
                     HDAQFWGGNLYNLWLSSLRALSPAADLSEPSKLGLPQVAATEAWGRRMLSAQLGSWAE
                     LRHDTLLYAKQSYTGVPSCDYPDAYIDPYPEFFAKLRAFAEAGSALTESFAATLGTRL
                     KVAQNYFKNLQQTMTLLGEMAKNQREGTPFTAEQLAFINGAVRILEEDAVCTTIDVPD
                     GWLADLYLDPQRSIEAAPTIADVHTQPADEAGNTVGKVLHVGAGWPRMMVTTIDTCQG
                     PRAYVGVTFAYHELETTNFERLTDEQWKTRLSTPQANPSWVEPILAQ"
     gene            9428..10069
                     /locus_tag="VFQ61_34015"
     CDS             9428..10069
                     /locus_tag="VFQ61_34015"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET9959574.1"
                     /translation="MSTNLEPRSLEPSSVIEVPPRVGSGTPGQSTPSVARLLVRLVIG
                     LLLIVGGVAVLGQLFRAELTALGQQFVARFGLTGMLFGTIIADGLHFPVPPQFYMLMG
                     ITSGVSAFATLLVINIGSFLGAWFAFLVATRVSVVTPVARRLEQARALTERTLRNYGS
                     WAIVVVSLLPITYAALCYLCGLTRVSKRQFALVTALRVPRLLAYYYLVKLGWV"
     gene            11070..>11802
                     /locus_tag="VFQ61_34020"
     CDS             11070..>11802
                     /locus_tag="VFQ61_34020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959575.1"
                     /translation="MAEFILIPEPIAEDAEAVVTALETAAIFGSRGDGAEALRWLKRA
                     AESSSDYGDDLRTLALARAAADLVAQIHAANTVPNGSVASLRSSEAPKPTDMQRRGGT
                     NGASQTPIPNGPGARSLSPSSAASRPPTNGVANGAAHANGAAHAHGQTNGAPVNGGPH
                     VNGSSSIQSGGSYGSPVGTGQSIIPTGSVPPASSAAYDAGSASASRSPSSPAGAERRL
                     PKPPSRPNSIRPLPTDNELTPTVLMN"
BASE COUNT         2178 a         3724 c         3706 g         2194 t
ORIGIN      
        1 ctggctggag cgtgcatccc aaccgacggg ataagcagat tgctctgcgg tatcgggcca
       61 cggctcgggc ttctctcggg tgacgatgca gcccgaaagc gccgtggtga cgaggacttg
      121 ccagcagcaa agcgccagct gtccgcgccg accaatcgat gccgcgagtc cgttctcgag
      181 cgctttggcg agccgcatgt cacggggcta gcggattggc tccgatgggg ccaacttccg
      241 tgcgcgctag gtcgagacct tctgcgcgag cccccgccgg actctgctac tctcagctcg
      301 gacagcgatg caccttcgga ttactttggc tttgggcttc ctggcttttg catgcagcgg
      361 tggggcgcag acgagctcac cgaaggccgc ggagccagcg accactcaat ccgcggcgga
      421 ccccgagctg agtcgcgcgg aaggtgaact gcgagaggct gaacagcagt tgtttggtgt
      481 gctcagggcc gagcgcgaga ccgttgcagc cgacgaatcg gccccggctc ccgcactccc
      541 agcgtccccc gcacccgcgc caaaggctca ggctgaagag atgaaacgcg ccaaacccct
      601 cgagcaacct cccggggaaa gtggaggagc agcggctgca gaaggcgatg gttgtcagtt
      661 cgggtgccgc gcgctgagct cgatgcgccg cgcttctgac gccatctgca ggataaccgg
      721 tgacgccgat ccgcgctgtc acagcgcgcg cgagcgcgtg cgggctgcgg aacaacactt
      781 gctgcgcagc ccctgcacct gtgccaagaa cgagggtgaa taagccagag ctgggctgac
      841 gccgggctac tcgtccgtga cgcaaccttc cgaagcagat ttcacgagcc gaacgtactt
      901 gtatagagcc ccgctcttca ccttcagcgg gggcgccacg aactgcgatt gccgacgcgc
      961 cagttcttcg ctcggaatca gcaagtcgat gcgccgctcg gtggagtcga tccggatttt
     1021 gtcgccattc tggaccaatg cgatcggacc tccttcctgc gcctccgggg tgatgtgacc
     1081 aatgatgaaa ccgtgcgatc cccccgagaa ccgcccgtcg gtgatcagcg ccacgtcttg
     1141 tccgagcccc gcccccatga ttaccgaggt gggcgagagc atttcgggca taccgggacc
     1201 acctttggga ccttcgtaac ggatcaccac gacgtgtcct ttgcgcacct cgcggcgctc
     1261 gaccgacgcg agcagatcct cttcgcaatc gaagacgatc gcgtcaccct cgaacaccag
     1321 gccctctttg ccggtgattt tggcgacaga cccggttggg gccaggctcc cgtacaggat
     1381 cgtgatgtgc ccagtgggtt tgatgggagc atcccatggg cggatgattt cctggtcggg
     1441 ggcgagccct ggcaagtcgc gcaagttctc ctctagggtg cgcccggtga cggtcaagca
     1501 ttgcccgtgc agcacaccgc gatcgagcaa gtacttcatc acggcgggga ttccgccgac
     1561 gctgtgtagg tcctcgaaca cgtacttccc actcggcttc aagtcagcca agaagggaat
     1621 tcggtcgctc acgcgctgaa agtcgtccaa gcccaggcga acgcccacgg ctttggccat
     1681 ggcgatcaag tgcagcaccg cgttcgtcga gccgcccagg gccatgatca cgaccatggc
     1741 gttctcgaac gcttctcgag tcatgatgtc gcgcggcttg atatcgcgct cgagcagcag
     1801 cttgatcgca gcccccgcgc ggacgcattc ctcgagcttc cccgagtcct cggcgggagt
     1861 gcacgaactg taaggcaggg ccatgcccat ggcttcgatc gcggacgcca tggtgttcgc
     1921 ggtgtacatc ccgccgcacg cgcctgcgcc tgggcacgcg tgctccacga tttccaggcg
     1981 ccgctcttcg tcgatgcgtc ccgcgagcag ctccccgtag ctctggaagg ccgaaatcac
     2041 gtcgagcttc gggcagtgct cgctcttgcc ggcccgaatc gtgccgccgt agaccatgag
     2101 ggccgggcgg ttcaagcgtc ccatcgcgat caaacagccg ggcatgttct tgtcgcagcc
     2161 cggaatcgag atgttcgcgt cgtaccactg cgccgacatc acggtctcga tggagtccgc
     2221 gatcaaatca cgtgattgca acgaatagct catcccatcg gtgcccatcg aaatgccatc
     2281 cgagactccg atggtgttga agcgcatccc gacgagcccc gcgccctcga ctccttcctt
     2341 caccttggcg gcgagcgcgt tgaggtgcat gttgcagctg ttgccctcgt accaaaccgc
     2401 agagatgcca acctgcgcct tgttcagatc ggcacgagtc aagccggtgg cgtgcaacat
     2461 ggcttgggac gcaccttggg acttgggctg cgtgattcgg gagctgtact tgttgagtgg
     2521 ctgggccatt atcgactttc ctggtgcgct gacgttctcg cgcgctagac gtttctccgg
     2581 tcccgattct acgaagcttc acgagcggca gctagcgcgg aacttgcccc gcgcgaactg
     2641 aatctgcagt cgccgcaagc ggtgtagtac cgcggggcag aatggaatgc tgacgcggtt
     2701 agagctgcaa accccagcgt gtcgccaggt aatcctcgag ctcctggcgt tcgcttccac
     2761 tcacgccacg cgcgtacagt atcagctcac cgagccggcc gtgataaggc gtgggacact
     2821 cgtcgtagac gtccttgccc acgaagtttt gggcccgcga taccgccgcc ggcacgagca
     2881 tcttgccgct gccggtgagt gtgcgattca gccgaagctc cacgttgccg gcgttgtctt
     2941 gcacgatcga gatcaagaac cggcgatcga cttcgaacgc atttttggcg ccttcgacga
     3001 agcttccgag cacctcgtag tagagcagat tcggcgtatg ccggccgaac tcgacgtcgt
     3061 ctcgatcatc accgttcgaa aagctgagga tacccgcgca gcccgcgttc ggcagtgctt
     3121 ccaccactgc gaagaagcct gcgccagaga agtccttgaa cccggccgga agcgcgagtg
     3181 cgtcgtccgc accatcgaac tcgagcaagg gtgggccgga ggcagacgca acgatgcggg
     3241 gcatttgacc cgagttggtc tggctcgcgt cgtggccttg gcccgattga tccgccacct
     3301 tcgagacgcg ttcgccctgc acgctgacgc cgcgatccgc catcagccac aacacgggct
     3361 gagccggcaa ttggggcaac gtgctcgtac ctcccgagct agtcgcggta ccgctcgtgc
     3421 tggctccgcc gctcgtgttt acgccgccgc tcgtgcctgc agcgttgccg gcgttccccg
     3481 aaccgccatg gctggtcgcg ccaccgccgc tcgaggcatt ccctcccgag cccccttcga
     3541 actcgccggc tgcgcccgcg ctgctctcac cacccgtacc aacttgcctg cccccgttcg
     3601 agctgctgcg cccgcccgtg cgaggcacgg ccgtatgcac gccaccggag ctgccggagc
     3661 ccgcgctcgt cgcgagctcc cccggcggat tgtcgagcag agaattgcag caaagcactg
     3721 gcaacgggct taccgcggcg agcaacccgc gagcggaacg agcgaaaggc gaaagcacgg
     3781 cacggaatgc ttagcactgc gcgctagcgc gatccgggtt aagacgcctg cccatgtcct
     3841 cagagcagcc gccaatcgtg gccgaaccgt tcgatccgat ccccgtcctt gccgcggagc
     3901 ttggcttgag cccgtccagc gtggcggcgg tggtgcgctt gatgagcgag ggcgccacgg
     3961 tgcctttcat tgcccgttat cgtaaagagg cgacgggggg cctcgacgaa gtgcaaatcc
     4021 gcacgctcgc ggaacgattc gagtacgtga ccgagctcga ggctcgccgc aaccacgtca
     4081 tcgaagagat ccaaaagcaa ggcaagctca gccctgaact gctcggcaag ctgcgcacgg
     4141 tgacgaccaa agccgaacta gaggatctgt acctaccatt caagcccaag cgccgcacac
     4201 gcgcgacgat agcgacggag cgcggcttgg cacccctggc cgagcagatg tggaagcaag
     4261 aggggtcggg gccgctagag cgcgctcaaa ggtacgtcga tccgagcaaa gacgtgcccg
     4321 acgccgcggc ggctttggcc ggcgcgcgcg atatttgtgc ggagcgcctg gcggaagacg
     4381 ccgagcttcg caaacgattg cgtgaggaat tcctcgagcg aggcgtgatt caggtcaaga
     4441 agaaggcgga attcgcggag caagtcacga agttcgatat gtatgcgaac ttcgaagaac
     4501 caatcgccaa agtgccatct catcgctatt tggcaatccg gaggggtgag aacgaaggcg
     4561 tgctctacgc tacgttgaat ctgccctcgg agccgtggat tccgttcgcg aagagccgca
     4621 tcccggtgcg tcccggaagt gcctgggccg cagagctgca gctcgtcgta gccgacgcgc
     4681 tcgatcgctt gctgctgccc tcgtgcgcca gtgaggctcg tgtggagctc aagttgcgct
     4741 cggatcagga agccattcag gtgttcgcgc ggaacctcgg cgagctgttg ctggcggctc
     4801 ctttgggagg gaagtccgtg ctcggaatcg accccgggca gcgtaccggc tgcaagtgtg
     4861 ccgtggtgga tggcacggga cgtctactcg aacacacgac gctctttcta gtgcagggcc
     4921 ccgctgcgct cgagaagtcc aagcaaacgc tgttggcgct gtgtcgtacc cacgagccgg
     4981 acgcgattgc ggtcggcaat gggactcacg ggcgagagac cgaggccttc gtgcgcgagg
     5041 tgctcggaga agcgggcctt ggccaagtcc cctgtgtttc ggtcagcgaa gccggcgcga
     5101 gtgtgtattc ggcgagtgac ctcgcgcgcg aggagtttcc cgagctggac ctcacggtac
     5161 gcggcgcgat cagcatcgcg cggcgcttgc aggacccgct tgcggagttg gtcaagatcg
     5221 acccgaagag catcggcgtc ggtcaatatc agcacgacgt cacgcaatct gcgcttggcc
     5281 gcaagctgga tgaagtggtg gagagctgcg tgaacagcgt gggtgtggag ctcaacaccg
     5341 cgagtgcgcc gctgctgtct cgcgtctccg ggatcggacc cggcttagcc aagcgcatcg
     5401 tcgcgcaccg cgacacgcac ggcccctttc cctcacgcaa agccatgctg aaggtgtctg
     5461 gtctcggccc tcgaacgttc gaacaagccg ctggtttctt gcgggtcagc ggcgccgagc
     5521 atccgctgga tgccagcgcc gtccacccgg agcgctacgc gctcgtggag cgtatggcca
     5581 aggatctcgg cgtggctttg gcggcgctcg tcggcaactc ggcgctggtg gctcgcctgg
     5641 agctcgatcg atacaggagt tcggatgtcg gcgacttcac actcgcggat atccgagcgg
     5701 agctcgacaa gccagggcgg gatccccgga aatccttcga gccgccgcag tttcgggacg
     5761 atgttcgcac cctgcaggac ctcgtagtcg gcatggatct gcagggcgta gtgaccaaca
     5821 tcacggcgtt cggcgcgttc gtggacatcg gcgtacacca ggatgggctg gtgcacattt
     5881 cgcaactcgc ggatcgcttc gtcaaagatc ctcacgagat cgtcaaggtc ggggaccggc
     5941 tgcgggtgcg ggtgctagag gtcgacatca cgcgcaagcg gatctcgttg acggcgcgca
     6001 gcgcgacccc gccgcagacc gcgagcccgc ggacggcgcc tgcgcccgtg cagaaacccg
     6061 caccaaaagc tgcaccaaac agatccaagc aagaggctcg tccggcaccg acacgcagcg
     6121 gcgcagactc gaccaacgcc gcgcaaggtt tcaagcacaa ccccttcgcg cgtctactcg
     6181 acaaaaataa gtgaaagatg atggtgcaca ggcctgtcat cgggcgtgtc ccagttcgaa
     6241 atcggccagc ttaatcgttg agagtcatcg accgttgcct ctcggcgtgt cccctccgca
     6301 cccgccccaa ccgccgctcc cgcttcacgt ggtcttggtt gacgacgacg cgcggcaaag
     6361 agagcagacg cagagttggt tggagagcgc cggctaccgg gtgagcccgt gtagtgggct
     6421 cggtgacttc gaacggctgg tcgcggcgac accaccggac ctcatcctga ttgatgtttt
     6481 gatgcctgcc tttggggcag cagacctgga acgtttactg cgtcacttcc cagccacctc
     6541 gagctctccc gtggtgctgc actcaccctt gccggccgcc acattgcgcc ggctggtgaa
     6601 gacttccggc gcattgggaa tcctcgaggc aactctcacg cgcgaggcat tcgtcgccgc
     6661 cttccgcgca tttgcagaga ccttgctctt gcggcaccgt ctgcgcgccg cgaacagcgg
     6721 aactcaccgg attgggtcgg gttcggccga acagaacggc gcgcaaggtc gggacactcg
     6781 ctctcggaac tcacgctgaa cgcctcggct gtgcgagcgg ttgcccaacg gccgcgctta
     6841 ctgcgcgagg atcggctcca cccagcttgg gttggcctgc ggcgtggaca ggcgagtctt
     6901 ccattgttcg tccgtgaggc gctcgaagtt cgtggtctct agctcgtgat acgcgaaagt
     6961 gaccccgacg taggcgcgcg ggccttggca agtatctatg gtggtgacca tcattcgggg
     7021 ccaaccggcg cctacgtgga gtaccttgcc gaccgtgttg ccggcctcgt ccgcaggttg
     7081 cgtgtgtacg tccgcgatcg tgggggcggc ctcgatcgat cgctgtggat cgaggtacag
     7141 atctgctagc cacccgtcgg gtacgtcaat cgtagtgcag acggcgtcct cctctaggat
     7201 gcgcacggcg ccgttgatga aagcgagctg ctcggccgtg aaaggcgtcc cttcgcgctg
     7261 atttttggcc atctccccga gcagggtcat cgtctgctgc aagttcttga agtagttctg
     7321 cgcgaccttc aggcgagtcc ccagcgtcgc ggcgaagctc tcggtgaggg ccgaaccggc
     7381 ctcggcaaag gctcgcagct tggcgaagaa ctctgggtac ggatcgatgt aagcgtctgg
     7441 atagtcgcag ctgggaacac ccgtatacga ctgcttcgca tacagcagag tgtcatggcg
     7501 cagttctgcc caggacccga gctgtgcgct caacatgcgg cgcccccaag cttcggtcgc
     7561 ggccacttgg ggcagtccca acttggaggg ctcgctcaga tcggcggccg ggctcagcgc
     7621 acgcagggaa gagagccaca ggttgtaaag gttcccgccc cagaactgcg cgtcgtgcgc
     7681 atcgacgagg tcgcgggcgg actcgagcgc ccccgggtaa cccgtgaact gagagagctc
     7741 cggcgcgagt aggctgagcg cctgattatt gcccagcgcg gcgtaagccg cgtccagcgg
     7801 gttcggcatc atgcggaacg cgggcagccg gtcgtaaacg acttggctga aaacatgcga
     7861 atcgatcacg tagcgttgtc cgaaaagcgc gaaactgcgg gccagtggca acgtcggaac
     7921 cacgccatcg ttgaccatga tttggctcgc gatcttctgc gcgccgaagc cgccttgcac
     7981 gatcgccgct tcgatctcga catcgctcct ctgcgccgcc ttttccgctc cgcccagacc
     8041 gtcgaccagt gcgttcactt ggccaaccgt catgttgtcg ctatccccga cgaacgtgcg
     8101 tagggcagag tcgatcaact cgtagtccgc cagagattgc tcactcatgc tttggtacaa
     8161 gagcagcatg gcttcgtact gaggtcgcaa aaaggtttgg ctcccatccg ggttggtctc
     8221 gatcaaacgg aagtccatcc gcccgagcca catcatggct ctgaagtagc ggctcaaggt
     8281 cgggtcctca tcgtagtgcc cgcggggctt gaattgagac gcatcgacga tgcgaggcgc
     8341 cccgaacagt acgaggttgg cttccccctg cgcatcggtc gccatcttca cgatctggga
     8401 gatgagcttg gtgtcgcctc ccgcaagagg cgcgagggtt ttcccagcca gcaacgaacg
     8461 cgctacggtc atgtacaagt ccaggtcagc cgctatcttg gggttggcgc tggcccccgc
     8521 ggcgactctc tcgtgaatgg cgctcaatag cgtgtcgagc ttcggaatca gcgcgccact
     8581 ctcgaaaagt atcagcaggt cctcgtacga agaatggacc gcctccagga tcgcgtcagc
     8641 gctcacgtaa acgggcaggt gctccgagta aatggccgca tagccgcgca cgaacgtcgg
     8701 aaatgccgaa cgtgtcgata ccacgaagcc gttctcgtcc aacttagcga gctcgcttgc
     8761 gttgagcttc agcgcggact cctgaatgcg atccaggaac tcggcgtctc caggtcgata
     8821 ggagagcttt tcgggtcgct tgagcgcgcg tttctctagg agctcttccg cggtcagccc
     8881 gtggttggcg tcgagcatcc cctgaagccg ttggagttcc tgcttttcgt ccgtgttcag
     8941 gtcttcgcga ccgcctacgg gctggcctcc cgctcctccg ctcgctttgc cgcccgaggc
     9001 cgctccggcg atcggctgag cgccacccgt gttctgcgtt cgtccccccg aggaatcaga
     9061 gcctccggtg gcgctggtgg ccccggcgcc gctcgagccc ccggtacccg tgtgcggatc
     9121 cggactggtc gagctacagg cgaccagcaa caaccacaac ccgaaggggc atgcagcgcg
     9181 gagagtcgtg cgacgtatca tgctcgctct ccagcaacgc ccatgccacg gcgcgaactc
     9241 gcgtatccaa ggacttagta gcgcccccaa ccgacttacc catggtcagt tcgaggcaca
     9301 gctggcacag actggcccag cagacgaacg agcttccgac gggcgtttcc cgcctgaacg
     9361 agcacagcga tccaagccgc ctaatatgca gccctgatgc tgggacgatg ctaattcgag
     9421 cgacgcgttg agcacgaact tggagcctcg gagtttggaa cccagcagcg tcatcgaggt
     9481 accgccccga gtgggctcgg gcacacccgg gcagtcgact ccttcggtag cccgcctact
     9541 cgtccgattg gtgatcgggc tgttgctgat cgtgggaggc gttgcggtct tggggcagct
     9601 gtttcgcgcg gagcttacgg cattagggca gcagttcgtg gcgcgcttcg gtctcaccgg
     9661 catgctgttc ggcaccatta tcgcggacgg gcttcacttc cccgtgccac ctcagttcta
     9721 tatgttgatg gggatcacgt cgggggtatc tgctttcgcg acgttgttgg tgatcaacat
     9781 cggctcgttc ttgggggcat ggtttgcgtt tctcgtggcg acgcgcgttt cagtcgttac
     9841 gccggtggcc cgccgcttgg agcaggctcg tgcgcttaca gaaagaacgc tgagaaatta
     9901 cggtagctgg gccatcgtcg tcgtaagctt gttgcccatc acgtatgccg cgctctgtta
     9961 cctctgtggc ctgacgcgcg tctcgaaacg ccagtttgcg ctcgttactg cgctgcgcgt
    10021 gccgcggctg ctcgcgtact actatctcgt caaactgggc tgggtgtgac cctgcgagta
    10081 tcggaccgcg gtgagccttc acgcgaggtc agttcttggt ccgatgtaag gtattaggtg
    10141 ccaaccgcca aactggggga ctcgcgcgct catcagggtt ggcgtggagc ggcggcccgc
    10201 tcgtccatat tggcgatcgc tgtgaagaat gctctgtgct cgggtgaaag ctttcgattg
    10261 tggacgtact cgttagccga attaacgccg aaaccgagcg gcgccttcca tgtcgttggg
    10321 tttcgcgaat ccaagccgtg agagggagcc agcgctgagt ggtaacccgc ggcaggaaat
    10381 cggattcgat cctagaaaaa aacgaggcta cccgttactc tgcggatcgt tctcgatttg
    10441 gttcgtcgag aatcgacggc gcgcctcggg cgcaaagccc agagactcca agcgacagtg
    10501 ctcgcgatat cgaactgcgc tcgtcgctcc agacgtagcg agttggttca gcaaccaagt
    10561 tgtgcagagc ggagagtccg aatagcgcat tcgaacagag cacagtcaaa agagcagcgt
    10621 tcaaaagagc aaagggcaac agagcgcaga gaggcaacag agcgcagaga ggcaacagag
    10681 cgcagagcca aaaagagtcc taaacgagca accccagaga cacgcgccat gctgagactg
    10741 ccatcctccg ttccgcaaaa cgcaggctcc accaaacggg gcgaggccaa gctgaatgga
    10801 ccggcggttt ggatgcgcag gcacggatac gcaaggtgga cgtcagtccg gaccacctca
    10861 gttaggatag ggttctctcc cagaggcccc gtgccctcga gtaggacatc tgtactaccg
    10921 ggtctttttc catggagtcc gacatctata ggagtcacct tggtggcgat tcgggtaggt
    10981 cggagcgagc tcttatcaaa ctaaatctgc tcgaatttat gagggtcgtt gtgtggattc
    11041 cgtcgtccca aagacgtaaa aggatatgaa tggcggaatt catcctcatc cctgaaccca
    11101 tcgctgaaga tgcggaggcg gtcgtcaccg cgctcgagac agcagccatc tttggaagcc
    11161 gaggagatgg agcggaggcc ctgcgttggc tcaaaagagc ggccgaatct tcgagcgact
    11221 atggggacga tctacgcacg cttgcgttgg ctcgtgcagc cgcggatctc gttgcgcaga
    11281 ttcatgcggc aaacacggtt ccaaacggga gcgtcgcttc attgcgtagc tccgaagcgc
    11341 cgaagccgac cgatatgcag cgccggggcg ggaccaacgg cgcgtcgcag actccaatcc
    11401 cgaacgggcc gggcgcgcgc agtttgagtc caagcagcgc ggcttctcgc ccgccgacga
    11461 atggcgttgc aaacggcgcg gcacacgcaa acggcgctgc tcacgctcac ggccaaacca
    11521 acggagcacc cgtgaatggc ggcccgcacg tcaatgggag ttcttcgatc cagagcggcg
    11581 gctcttacgg ctctccggtt ggcacgggtc aatcgatcat accgaccggt agtgtcccgc
    11641 ccgcgagttc ggctgcgtat gatgcaggct ccgcgagcgc aagtcgctca ccttccagtc
    11701 cggcaggcgc cgaacgtcgc ttgcccaagc cgccctcgcg tccaaattcg attcgaccgc
    11761 tcccgaccga taacgagctc acaccaaccg tgctgatgaa tg
//
LOCUS       DASTKU010000960        22453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1530131, whole genome shotgun sequence.
ACCESSION   DASTKU010000960 DASTKU010000000
VERSION     DASTKU010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 22453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22453
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530131"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFQ61_34025"
     CDS             complement(<1..571)
                     /locus_tag="VFQ61_34025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET9959576.1"
                     /translation="MNPGKSVKLEIDGQTLELPLLEGTEGERGIDISELRNKTGCITL
                     DSGYGNTGSCTSSVTFIDGEQGILRYRGIPIEELAENSSFVEVAYLLIYGHLPSDSEL
                     QRFSRLLTENEMLHEDMKLHFEGFPPNAHPMAILSAMINASSCYYPSLMQRASPAQFD
                     VHAARLLSQVRTIAAFSYRKSRGLPIIYPK"
     gene            1036..2850
                     /locus_tag="VFQ61_34030"
     CDS             1036..2850
                     /locus_tag="VFQ61_34030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9959577.1"
                     /translation="MRRDQPELVDEEALAWRNQLTLRLVRSFLFVFLLSAPLTWLALH
                     GALERYVLTTITFLGAVTVGVPAITGRPAGATRSWMVIVPALSASIAGFAMVGFLSGP
                     GVALTITLMLSGLLLGRRVMVSLILISALIVGGIAWAAVTGHIAPPPSADLAMTKAVP
                     WLRSVGVTFLGISLFGSLMVSFIGRIESSLRLAQSETLRREQAERERAEAQIAALQAK
                     QLETMGRLAAGVAHDFNNSLTTIIGSAELLKLELEERTGLVELADAVLHASRHAAELT
                     RQLLAYSRKAQMLLAPTDLHRIVEEAVSFARRSVNPQINIVAELHADSSTVAADPTLL
                     RSAILNLLVNASDAMPEGGKLTVTTSTIRLPNQPPETGTWFVLEVLDTGVGIARELLP
                     HIFDPFFTTKAIGKGTGLGLAAVAGTVKAHGGKIEVDSEVGCGTVFRVKFPCLATGAH
                     VAPPEATGIVHGDGEILLVDDDAMVSLAAIASLESFGYRVTRAADGASALDIVRANRH
                     RFRLILLDLRMPGMSGEATFDALSRLAPETPVLIWSGYAAEQDVHGMLRRGARGFVQK
                     PYRLAELSQAVAAAIGIPRRADEVPGARARSDGTRPSV"
     gene            complement(2869..3711)
                     /locus_tag="VFQ61_34035"
     CDS             complement(2869..3711)
                     /locus_tag="VFQ61_34035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959578.1"
                     /translation="MRFTTYFLAASSLTLGLTIPNQARAQTAIECAESFEQGQVDRTR
                     LHLSDAKRAFAMCSQLACPQPIVKDCVEALEAVQRELPLFGFRVRDAEGHELLEVRVR
                     LNGELLTDRLDGSMYQRDPGLYRVEFEATGFKPLALTLTLHPGERTRVVDVQVESTSA
                     KAQPKQGVTLEPTPPPPPFRVPLESIITAGAGLAALGGFAYFRVRASQGYDSLESCRP
                     YCSDERVEEVRTRYVLSSVALGVSGAALATAGVSLWLLQPRKERPTVALSVGLTHAVL
                     SRSF"
     gene            3819..4319
                     /locus_tag="VFQ61_34040"
     CDS             3819..4319
                     /locus_tag="VFQ61_34040"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9959579.1"
                     /translation="MEQIDSYLELARRAARESFVAKCPGFYLLKRPRTGPKPTSPAPQ
                     IRFETWQEKMEIDPFAAEWRIAPVRKKPDNPFPERLVVGRAPNCDVVLRVPFISKVHA
                     HIMVQADGLTLVPGQSQKVQYNYRPIEEGGRRRLLVGDHIAFGALEFAFVDSAMLYDI
                     LRTEVR"
     gene            4404..6047
                     /locus_tag="VFQ61_34045"
     CDS             4404..6047
                     /locus_tag="VFQ61_34045"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9959580.1"
                     /translation="MSLDPNEVRGSTGTPSVTAVDSSAAEKYEVLVELGRGGSGVVSL
                     ALSRGIGGFNKLVVLKTIKDDLRDREAYGKMFLEEAKLAARMSHPNVVQTYEVYKHKG
                     LPVIVMEYLEGQAFSEVRRRAVSDPRLDENMTISILCKVLAGLHYAHTLRDFRGTALK
                     LVHRDATPHNVLVTYQGQVKLLDFGIAKLNASRERTNTGVIKGKLDYMPPEQLQGDDL
                     DCRSDLFAVGIMLWEGIARRRLWGDANDGEVMRRLLNGDIPSIREACPDLDPELARIC
                     EHALAPDPDARYADADAFRADLERYLATRGGLVPDSVIGQLVTETCAEPRQRLQASLD
                     SKLRRLTADPATGEEIQRALESLRVRGVDAAELATTSARTIELPRRERRRWGWGLAAG
                     GFAAALALLGYFGWAPRSAAWARTTAADVVVAPSAEAALRNSSSAQATARPRIPEPPA
                     AKAVPQDFKAEGTTTEKIPAPSTSEPHARVLKGGPLGALRSGVTSGSAPAVSAAAGGR
                     NPPPKVSTNACDPLYVIDEFGIKRYRRECLPGAGAPHAN"
     gene            6489..7907
                     /locus_tag="VFQ61_34050"
     CDS             6489..7907
                     /locus_tag="VFQ61_34050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959581.1"
                     /translation="MPASCHDARIDAGETDVDCGGETLCERCEEMRSCRVDADCVAHY
                     CSQGACLSPHCDDGRLNADESDVDCGGDSACARCLDAQACLQDGDCASRHCAEGACIS
                     GTCFDGAANSLESDVDCGGANACPRCADGLACADDTDCSSATCAGGRCVPASCHDASS
                     NNGETGVDCGGVTLCSRCPVGSSCETGADCASGHCVRGACVPENCDDGRVDGAESDLD
                     CGGETCAPCADGAICRRDSDCQSRHCYAGSCGTSQSCDYPAERGRNPRKVRLVYMVPA
                     DRAVEPQYIASLERGIRHLQAFTATQLLNGQTFSLGELIVTTVQSSHDSAWFNTPRAD
                     SALRWSFYRNVLDDGRALVSAKNDGNQLWLFFPDVLPTCDSELGSVDRYFGVASMNAL
                     RGLSGLAPLPNCGGDPVPAAGTCQSIGDLNGLLAQALEIAPAESADYPSPFPSALVSA
                     YDHSPFFAPEYFAAAPCECADW"
     gene            complement(7942..9846)
                     /locus_tag="VFQ61_34055"
     CDS             complement(7942..9846)
                     /locus_tag="VFQ61_34055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959582.1"
                     /translation="MLGCGAPVTAPSPTAGCDNELLDGSETDVDCGGSNLCQRCAGDH
                     RCQDDSDCATRQCVHGLCRSTGCDDKRKNGVETDTDCGGDNECARCEVTKRCREASDC
                     ESAICEQGICRAKPCENGVRDGFETDVDCGGVGECTRCDDGLSCEEDDDCSSRFCAAS
                     LCAPAPTCDNGRFDGAETDLDCGGECSPCAPGGLCSNNADCESEWCLDGTCQAPDACH
                     DGQKNGDESDLDCGGSCDACPVENKCNADADCATSRCRLSRCVADPCTDSRVTGSETD
                     VDCGGETECQRCAEGQHCIQASDCASRMCIAERCQSSNCADGIQNGRETDVDCGGPDC
                     GTCWSGMGCIVDSDCVDGGCWDGKCPLSHCTDQRRNSDETDIDCGGSSNCHRCSNGQV
                     CWADRDCRSGHCSAGTCAPPGVCNYDRSRSGTNARDVRLMYLVPTAGAAYPNALVAFE
                     RAARNMQFFLRTQLQGPTFTLGSPAPIVQTLPSPDWLSRARGPEPSPRDFFSNVIEVA
                     EPLGARLDDPDHLWLIAAQVAVPCDQDLSPDGGVVLLANPELRALSGMLPLAADCGVV
                     KSESPINPCHAVGALTAGFMRALGLGYPAWEDYPSPLPDSTRVTLLTSPFFRTEKLGD
                     ATCACDSLVY"
     gene            10244..12019
                     /locus_tag="VFQ61_34060"
     CDS             10244..12019
                     /locus_tag="VFQ61_34060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959583.1"
                     /translation="MLSCLLGAQNCVPDLDGFGVAPQCGDGSRDADESDVDCGGVNLC
                     LRCDDGRRCTRDSDCQSQYCLEGSCASARCADGEKNGGEVDIDCGGSTPCKRCGAGQR
                     CEQDGDCLTGHCEQNACALSSCSNQLQDGDESDPDCGGGCAGCADGQRCSADSDCGSQ
                     HCEAGRCSPSKCHDQRVNGGESDTDCGGETSCPRCALGAACTRDLDCATATCVDRACA
                     PASCANQILDGSEVEVDCGGNDGCSGCLDGLRCERDRDCTSSYCFDHLCRPAHCASGL
                     VDGSETDRDCGGDNACLRCQLGQACSSYLDCDSGHCEHQRCVPETCSDGQLSPGESDV
                     DCGGDECAPCLDRSICARDSDCVSQHCYDARCSPTMACEYTGAFGENVRQVHLVYLVP
                     ADREVRANYIANLERSIRHIQVFTAQQLPDHRTFSLESPVVRVLKSKRPGSWFSTGSG
                     SGGSSLAFRDNVISETGALLGRSFGTDPWLIYADAEPACDQNTDDAWGKQRVAGASVL
                     RGISGVDNVDACSGGARLPTEPCAYTGRMARSLIRGLDAEYLDYLAYPTPFTSYLASS
                     LDRSIYFGVEDVGDRYCDCSVVVPE"
     gene            complement(12082..13629)
                     /locus_tag="VFQ61_34065"
     CDS             complement(12082..13629)
                     /locus_tag="VFQ61_34065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959584.1"
                     /translation="MRVWVGSIGAVILTLSCGDGGAGHGQSINDITAEGGADDIDAAG
                     GALVSSGGRGTPQGGTRATQQGGSTTLHTGGASGVLTSTNASERGGTSNITYHAGGSA
                     EAGETGSGGERELPTGGGTTSTTGGQPGTVVSCAGALTFADPTLEYEVRKATGRTNGA
                     LLAEDVAGLTTLQLARTGVSSLSGIECLRDLTVLELSDDPVMDLTPLSNASELQELLL
                     DGSSVSELAPISHLPALKKLDLTRSSVFDITPLTTVTSLETLAISRISPGNMDAFLGL
                     TNLRSLTADYCGIDSLSALSELHQLRELSLYHNIFTDIAPLAQLTTLEKLNLGENWFT
                     DISPLSALDRLTELELAFDTIPDITPLAALPQLRRLSIEGVQEIADFTPISALRSLTE
                     LTVSYSGLDSTLPLVELTALTKLKFSGGHLSELGPLANLQGLTELVLTDNEISEVGPL
                     SVLPLLSSLDLTMNKIRQIAPLAMTEALGPGDYLGIGFNHLDCQEQKQYLDALTQRGV
                     QLFTDCE"
     gene            complement(13880..15505)
                     /locus_tag="VFQ61_34070"
     CDS             complement(13880..15505)
                     /locus_tag="VFQ61_34070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HET9959585.1"
                     /translation="MGLWDTIKSWFGVSSPIPDTSPAPPSAAAGSPSQLSASGPRPEP
                     GPGAARAEAPRSTPKTNSPDRFEAPGILGLSAAQLRIRALKINPFATPWIGRVDTIPP
                     QTDERTALIDRGLVLRGLLSVAQLREIHRVGDLWLKFRDARNLAVSYGRQRAEEVIQQ
                     RRAEHAQKKQLKRQEAAARRQAEQQARAARKANDIIFAGRGVSHRLGNRRSQIEDLTR
                     AGLPLLATPADLAQALGITVPALRWLCFHHESPERTHYVYFEIPKRSGGTRLIASPKS
                     QLRRTQRWIFDTILSRLPVTEAAHGFVKGRSTVSNAAPHQGQHVLVNLDLADFFPSVT
                     FPRVRGLFESLGYSPAVATLLALLCTESARTLVEFEGRKHWVAVHDRALPQGACTSPT
                     IANLVARKLDRRLGGASRKLGFEYTRYADDLTFSAESSAANVGLLLARVRHIVKEEGF
                     ALNPKKGRVQRRSRRQEVTGVVVNDKLSVARDELRELRAILHNARRTGLQAQNREQRP
                     HFEAWLRGRIGYVMMIDPIKGRALAAQLNACSP"
     gene            complement(15808..17460)
                     /locus_tag="VFQ61_34075"
     CDS             complement(15808..17460)
                     /locus_tag="VFQ61_34075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HET9959586.1"
                     /translation="MRVDLRYSGHSGLRELASGSELSFAPNLARPKVFFDAELVSPLR
                     FREAISALHGVVVGDLKFHARDKSAYLAWKAQQEADEAALRKAITEQETQAELARLGT
                     EPEPAGLEPEFRKMHGLYWKARRKWAAELSAHDPALFRALVPCDPVVTVAPDVVAFEC
                     FSKDESCYGCLSVDRGAFRNTGATGLGTTNVDYSLALYEHFQTLRSYRPTRLLVDPSG
                     FEVRAGSGLREEKIDLPASWLRGFGQLQAAMMLPAERVSLAPEALYSVLSLLRRRREK
                     TGPRSLKFILTPGKSATLVVEPWGTEIAGRGAPYTGPKPLEIKVWGRRRLLALARVLP
                     LVESVEVQLLGTGMPSIWVLRMGEMSFVLALSGWTANDFTSGTNLDAAFAGASPSAAL
                     MEQIRFQLEERRSASLPELLANGLTRAETMAALHTLAKRGQVMYDFVTAQYRFRPILP
                     EELGEQTLGPPPPEQAQGIRLASGVVIQRQEPLELGKRLYVARVQETTCEAVIDSDGQ
                     LSRARCSCSFFFKNRLRSGPCRHLLALRLHSQGFSSISPGNA"
     gene            complement(17457..19436)
                     /locus_tag="VFQ61_34080"
     CDS             complement(17457..19436)
                     /locus_tag="VFQ61_34080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959587.1"
                     /translation="MPRLEFTYSGRGYQFTRFFEAELRGSTVLYCSGGSDVATRRTEK
                     QYSTEDEAYGAYARSLRGKRRAGYVAAGPAREIEGCTPPRASSALLIDVYFAAGDARF
                     LEEVLHFDGARKLAELAEPWFEDPRPFARQALLAYVDDGCHRAEHKGLVKRLFKLAEG
                     ARDTELMAHFLVAFDRLTRRPLLTVGHTWEPETGKARPILELRNDPLVPERIAPAARA
                     GARAKSPSGSTKAKSELAEFTRSTRRYLVRRALRYFRKIGYRAPLAYRAGVLTALRLY
                     RDEHLNTVGRLLSAWGLMHVLYGRSPVLRRRPNGLLLADGRTLAELSPMPLFPAAWES
                     AFSDLLQLVQGAPSRTVRAFALELLRSGYTKPLAALPFSDIKSLVLAEHEEAQVLGVE
                     LFLRAPGLERVTIQDWLELLGAENLEVLAAVCDRAAALVSGTRLGLDACIQLALAPAA
                     PLAALGFAWLKEKPIRTRLELEALLRLARAKVASVRKDAVHYVAGLLMSAPFAERSHT
                     RELCDAQHVEVRGEGLRVAAARFGEDIELWSELCESPYPDVRSFVLQRAALFRHAAPE
                     SLARVFAATLCALAGASRDKQRVSRELLERLAATPSEAPTLLPLLRVVLMSVHPAERN
                     LGLVTLTRAALRDSELRRLTQQSFPELSMALQVTQ"
     gene            19752..20282
                     /locus_tag="VFQ61_34085"
     CDS             19752..20282
                     /locus_tag="VFQ61_34085"
                     /inference="COORDINATES: protein motif:HMM:NF016382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HET9959588.1"
                     /translation="MTAPAIPQPIAILDVQYFQDKASAACVVAQHWSDGEPSEVKHRI
                     VSPVAPYEPGRFYLRELPALLAVLGTLETPVSAIIVDSYVVLDAAGTLGLGGHLYRHL
                     DGERPVIGVAKTRYKGSSFALPVLRGQSQRPLFVTALGVAAERAAEWVKAMHGEYRIP
                     TLLALADSTARAGANI"
     gene            21005..22126
                     /locus_tag="VFQ61_34090"
     CDS             21005..22126
                     /locus_tag="VFQ61_34090"
                     /inference="COORDINATES: protein motif:HMM:NF026241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M91 family zinc metallopeptidase"
                     /protein_id="HET9959589.1"
                     /translation="MMLTRAEFVNELTRTNRDIYLGAPSKVRTQSSMLATVNAVAAFE
                     ASGTGANLLALMRTVGAVPQVKKNRYTNALNALYQSFPSPIYVTMNPLTISLAMVNGI
                     GVPRSNNVPSHQVDVLLALQELHNTGSGNALLAAICAEVNKGGNGNRVAIGDAVGTMS
                     GGNECQIVGKPPDDYQTELAAALVGDHSKVGAKIDQALTKMGKRGQYEWLQERINNEP
                     IWQLQGEPSTVGSASVNGNNWISVEMLKSWTERRSSFPAPLKAGRASDAQVVLGAILH
                     PGATPGPGGSTKVRWSAANLMAAGAARPPCVGLAHELVHALHNMRGDQPGNDNGHPTT
                     ALFEYICVGLGPWHDEPNTENAVREGIPCTRRPYYAPGG"
     gene            complement(22163..>22453)
                     /locus_tag="VFQ61_34095"
     CDS             complement(22163..>22453)
                     /locus_tag="VFQ61_34095"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HET9959590.1"
                     /translation="RMPAVVSFLFGLVHGLGFAGALKDIGLPPQHLPFALLSFNVGVE
                     VGQVFMVGLAYALLRLPVPRAWYTHARRPALYAVGTVAAYWSFQRISVLVLS"
BASE COUNT         4146 a         6979 c         7218 g         4110 t
ORIGIN      
        1 gcttgggata gatgatcggc aggccgcgag actttcgata cgagaaggca gcgatggttc
       61 tcacttgcga caacagacgc gcggcgtgca cgtcgaactg cgccgggctc gcgcgctgca
      121 tgaggctcgg atagtagcag ctcgaggcgt tgatcatcgc ggataggatc gccatcgggt
      181 gggcgttggg tggaaagccc tcgaagtgta acttcatgtc ctcgtgcaac atctcgttct
      241 cggtcagcag tcgagagaag cgctgcagtt cggagtcgct cggcaagtgg ccatagatga
      301 gtaggtaggc gacctcgacg aagctggagt tttctgcgag ctcttcgatc ggaatacccc
      361 gataacgtag aataccctgc tcgccgtcga tgaacgtgac gctgctcgtg caggagccgg
      421 tgtttccgta accgctatcg agcgtgatgc agccagtctt gttccgcagt tcggagatgt
      481 cgatgccgcg ctcgccctct gtaccttcca gcaacggaag ttcaagggtt tgaccatcga
      541 tctccagctt gacgctcttc ccaggattca tggacgaaat ttccgcgatt ggcgggctgc
      601 tggcaacagg gcgcgattgg cacgtgcgtc tcgtcagcat tcaccgtcca accgggcggg
      661 cgcgtcgaac ccgttttccg ggggcggcgt aactcgccag tcccgaaccg cgcattggga
      721 gctgccgtcc cgagccgttc cagagacgac tcgaggtccc caacccgact ttctagagtt
      781 tcgaaggttg aaccgtcacg aatcgtagcg atcaaacagt ccagaaacag gctcgacaag
      841 attgcctcgc ttatcggtca ggagaaaacc caatctgttt tcgagcggtg ccatcgaacg
      901 gagcgaggct ctttccccac gatcgaggtg gcatgccact cggatgtgag gggcagccgc
      961 cgtcccgagc aaccgaactg cccaaattcc caaactcctg tcgcgcgggc gcgcgctctt
     1021 tcgtattctc cggacatgcg gcgggatcag ccggagctgg ttgacgagga ggcgctcgcg
     1081 tggcgcaacc agctaactct gcgcctcgtg cggagcttcc tattcgtgtt cttgctcagc
     1141 gcaccgctta cgtggttagc actccacggg gctttggagc gctacgtgtt gaccacgatt
     1201 acgttcttgg gtgccgtcac ggtcggcgtg ccggcgatta ccggtcgtcc cgcgggcgca
     1261 acccggagct ggatggtgat cgttcccgct ttgtcagcgt ccatcgccgg ctttgcgatg
     1321 gtgggattct tgtccggacc aggggtggcc ctcaccataa ctctgatgct gagcgggcta
     1381 ttgctcgggc gtcgggtgat ggtgagttta atcttgatat ctgctttgat cgtgggcggt
     1441 atcgcctggg ccgcggtcac tggtcacatc gcgccgcctc cgagcgccga tctcgcgatg
     1501 accaaggccg tgccgtggct gcgctcagtc ggtgtgactt tcctcggaat tagcctgttc
     1561 ggtagtctca tggtttcgtt catcggccgg atcgagagtt cactgcgctt ggcgcagtcc
     1621 gagactttgc gccgagagca ggcagagcgt gagcgcgccg aggcacagat cgcggcactg
     1681 caggccaagc agctcgaaac catgggccgc ctggcggccg gagtggcgca tgacttcaac
     1741 aacagcttga ccacgattat tggatctgcc gagctcctga agctcgagct ggaagagcga
     1801 acaggactcg tcgagctcgc ggatgcggtg ctccacgcgt cccggcacgc ggcggagctg
     1861 acgcggcagc tgcttgcgta ctctcgaaag gcgcaaatgc tgctcgcacc gacggacctg
     1921 caccgcattg tcgaggaggc tgtctctttc gcgcggcgat cggtgaaccc gcagatcaac
     1981 atcgtggctg aactgcacgc cgattcgtcc acggtggccg cggatccgac gctcctacga
     2041 agtgccattc tgaacctgct cgtcaacgcc agtgacgcca tgcccgaagg gggcaagcta
     2101 accgttacga cgagcacgat tcgactcccg aatcagccgc ccgagacggg gacttggttc
     2161 gtgctcgaag tgctggatac aggggttggt atcgcccggg agcttctccc gcatatcttc
     2221 gatccattct ttacgaccaa ggcgattgga aaaggaacgg ggctgggcct cgcggcagtt
     2281 gccggcacgg tgaaggcaca cgggggtaag atcgaggtcg atagcgaggt gggttgcggt
     2341 acggtgttcc gcgtcaaatt tccgtgttta gcgaccgggg cccacgtcgc accgccggaa
     2401 gccactggca tcgtgcacgg tgatggcgag atcctgttgg tcgatgacga cgcgatggtc
     2461 agcttggccg cgattgcgtc tctggaatcc ttcggatatc gcgtcactcg tgcggcggac
     2521 ggtgcgtcgg cgctggacat cgtgcgtgca aatcgacacc gctttcggct catccttctg
     2581 gatttacgca tgccgggcat gagcggcgaa gccacgttcg acgcgctctc gcgactcgcg
     2641 ccggagacgc cagtcctgat ttggtctggg tacgccgccg aacaggacgt acacgggatg
     2701 ttgcggcgcg gcgcacgtgg cttcgttcag aagccatatc gcttggcgga gctgagccag
     2761 gccgtcgcgg cggcaatcgg aatcccacgg cgcgcagacg aagtccccgg tgcgcgcgca
     2821 agaagcgacg gaactagacc atccgtttga ggggcgaacg atcgctgctc agaaactccg
     2881 gctgagaacc gcgtgcgtaa ggcctacgga gagcgcgact gttggccgct ctttacgcgg
     2941 ttgcagtagc cacaaagata ccccggcggt agccagcgct gcgcccgata ctcccagcgc
     3001 cacactcgaa agtacgtacc gtgtacgtac ttcttccact cgctcatcgg agcaatacgg
     3061 ccggcaggat tcgagcgagt catagccctg acttgctcga acgcgaaagt acgcgaaccc
     3121 gccgagcgcc gcgagccccg ctcccgccgt gattatgctt tcgagcggaa cgcgaaaggg
     3181 tgggggtggg ggagtcggtt ctagggtgac accctgtttg ggctgcgcct tggccgacgt
     3241 cgattcgact tgaacgtcca cgacgcgcgt ccgctcaccc ggatgtagcg tgagcgtgag
     3301 ggcgagtggc ttgaatcccg ttgcctcgaa ctcgacccga tagagcccgg gatcgcgttg
     3361 gtacatcgac ccgtcgagac gatcggtgag caactcaccg ttcagccgca cccgtacttc
     3421 gagcaattca tggccttcgg cgtcccgcac ccggaacccg aataggggga gttctcgctg
     3481 gactgcctcg agcgcctcga cacaatcctt cacgatgggc tgggggcacg cgagctggct
     3541 gcacatcgcg aaagcgcgct tcgcatcgga gaggtgcagt cttgtgcggt cgacctgccc
     3601 ctgctcgaag ctctccgcgc attcaatcgc ggtctgggct cgagcctgat tcggaatggt
     3661 caggccgagc gtgagtgacg atgctgcaag aaaataggtg gtaaagcgca tgctccgccg
     3721 cggacctggt tacggacggg acgcgcaacg cggtaaggcc cagcgtcagg gccaagatat
     3781 tacgagaatg gtggccactg cagtagtaca ctgcgcatgt ggagcagatc gacagttact
     3841 tggaactggc acgcagggcc gcgcgcgaat cattcgttgc caagtgcccg ggattctatc
     3901 tgctgaagcg gccccgtacc ggcccgaagc cgacaagccc ggccccacaa atccgcttcg
     3961 aaacgtggca ggaaaagatg gaaatcgacc cgtttgccgc ggagtggcgg atcgcgccgg
     4021 tacgcaagaa gccggacaac ccgtttccag aacggctggt ggtaggacgc gccccgaact
     4081 gcgatgtcgt cttgcgagtc cctttcatct ccaaagtaca cgcccacatc atggttcagg
     4141 ccgacggcct cacattggtc cccggtcagt cacagaaggt acagtataac tacaggccca
     4201 tcgaggaagg cggccggcga cggctgcttg tcggggatca catagccttt ggtgccctcg
     4261 agttcgcgtt cgtggattcg gcgatgctct acgacatcct gcgcactgaa gtccgatgac
     4321 gcataaagcg gcaatgctcc ccgccgttct catcatcgag aaccgctcga gtgacgccgc
     4381 cccccgctcc tcggtcgcct gccatgtccc tagatcccaa cgaagtccga ggaagcaccg
     4441 ggacgccgag cgttaccgcg gtagactcca gcgcggctga gaagtacgaa gttctcgtcg
     4501 agctcgggcg gggtggatcg ggcgtggtga gtttggccct ctcgcgcggg atcggaggct
     4561 tcaacaagct cgtggtcttg aagaccatca aggatgatct tcgagatcgc gaagcttacg
     4621 gcaagatgtt cctcgaagag gcgaagctgg cggctcgcat gagccacccg aacgtggtgc
     4681 aaacttacga ggtctacaag cacaagggcc tccccgtcat cgtgatggag tatctcgagg
     4741 ggcaggcctt ctcggaggtg aggcgtcggg cggtgagcga cccgcggctc gacgagaaca
     4801 tgacaatcag catcctgtgt aaggtgctcg cgggtctgca ctatgcgcac acgctccggg
     4861 actttcgcgg tacggcgctg aagctcgttc atcgcgacgc cacccctcac aacgtgctcg
     4921 tcacttacca aggtcaagtc aagctgctgg acttcgggat cgccaagctg aacgcatcgc
     4981 gcgagcggac gaacaccggg gtgatcaagg gtaagctcga ttacatgccg cccgagcagc
     5041 tgcagggtga cgacctagac tgcagatctg acctgtttgc ggtgggaatc atgctctggg
     5101 agggcatcgc gcggcgtcgg ctgtgggggg atgcaaacga cggggaggtg atgcgtcgcc
     5161 tgctcaacgg tgacattccc tcaatccgag aagcatgccc agacttggat cccgagctcg
     5221 cgagaatttg tgagcatgcg ctggcgcctg atccggacgc gcgatacgcc gacgccgatg
     5281 cgttccgggc cgatctggaa cggtatctgg ccactcgcgg agggctcgtc cccgactcgg
     5341 tcatcggcca gctggtgacc gagacctgcg ccgagccccg ccaacgatta caggccagcc
     5401 tcgattccaa gcttcgacgg ctgaccgccg atcccgccac cggtgaggag attcaacgcg
     5461 cgctcgagag ccttcgtgta cgcggcgtgg acgcggctga gctagcgaca acttcggctc
     5521 gcacgatcga gctacctcgt cgcgagcgac gccgctgggg ctgggggctt gcggccggcg
     5581 gcttcgcggc ggcgctcgcc cttctaggct acttcggctg ggcaccaagg tctgcggcgt
     5641 gggcgcgcac gaccgcggct gacgtcgtcg ttgcgccctc tgccgaggca gcccttcgca
     5701 actcttcgtc agctcaggct acggccagac ctcggattcc ggaaccaccc gcagcaaagg
     5761 ctgttccgca ggacttcaaa gccgagggca caaccaccga aaagatccct gcgccatcca
     5821 cgagcgaacc ccacgcgcgt gtcctcaagg gtgggccatt gggcgcgttg cgttctggcg
     5881 tcaccagtgg ttcggccccg gccgtgagcg cagccgcggg cggccgcaat ccaccgccga
     5941 aagtctcgac gaatgcttgc gacccgctct acgtcatcga tgagttcggc atcaagcgtt
     6001 accgtcgaga gtgcctaccc ggcgccggtg cgcctcacgc gaattgaagg cgcgccttgg
     6061 tgatgcgggc tcggcctcga tctatcgcgc gtcacgggct cgggcccgga tgggcagtcg
     6121 cgctgcgtgc aatcggacta gcggggctga gcgcgagcgc atgcgttcct gacttggagg
     6181 tcacgcgtgt cgctcagagc tgcgtcgacg gtgcgcaggg tgaaagcgaa acggacgtcg
     6241 attgcggagg gataaacctg tgcccacgtt gccgtctcac cgctcgttgc gaacgagatt
     6301 ctgactgtat cagccgctac tgccaagctg gtatttgttc cgctgccagc tgcactgatg
     6361 gcgtgcggaa cgggcgggaa agcgagatag attgtggggg gccaacgact tgcgagcgtt
     6421 gctccgacgg tctcggatgt gacgccaact ccgattgcgc gagcggagcc tgctccgagg
     6481 gcacctgctt gccggcctct tgccacgacg cgcgcataga tgcgggcgag accgatgtgg
     6541 actgcggtgg tgaaacgttg tgcgagcgct gtgaggagat gcgcagctgc agagtcgacg
     6601 cggattgcgt cgcccactat tgcagtcaag gcgcgtgctt atcgccgcac tgtgatgatg
     6661 gtcgactcaa cgcggacgag agcgatgtgg actgtggtgg cgattcggcg tgtgcacgct
     6721 gcctcgacgc gcaggcctgc ttgcaagatg gcgactgcgc cagtcggcac tgcgccgagg
     6781 gtgcgtgcat atcgggcact tgcttcgacg gagccgcgaa ctcgctcgag agtgacgtgg
     6841 attgcggggg cgccaacgcc tgcccccgtt gtgcggatgg tctggcttgt gccgatgata
     6901 cagactgttc cagcgcgacc tgcgctggcg gccgctgcgt tcccgcgagt tgtcatgatg
     6961 cgtcgagcaa caatggcgaa acgggcgtgg actgcggcgg cgtgacgctc tgctcgcgct
     7021 gcccggtggg aagctcttgc gagaccggcg cggattgcgc gagtggccat tgtgtccggg
     7081 gcgcgtgcgt gcccgagaac tgcgacgatg ggcgcgtaga cggggcggag agtgacctgg
     7141 actgcggcgg ggaaacctgc gcaccttgcg ccgacggcgc tatctgtcgg agagactcgg
     7201 attgccaatc tcgacattgt tacgcaggct cgtgcggaac cagccagtct tgcgactatc
     7261 ccgccgaacg gggccgaaac ccgcgtaagg tgaggttggt atatatggtt cccgcggatc
     7321 gcgccgtcga gccacagtac attgcgagtc tcgagcgcgg aattcgacat cttcaggcgt
     7381 tcaccgcaac gcagctccta aacggacaga ccttcagcct gggtgagctc atcgtcacca
     7441 ccgtgcaatc cagtcacgac agtgcctggt tcaacacgcc acgggcagac tcggccctgc
     7501 gctggagctt ctaccggaat gtgctggatg acgggcgggc gctggtgtcc gccaagaacg
     7561 atggtaacca gctctggttg ttctttcccg acgtgctgcc cacctgcgat tccgagcttg
     7621 gcagcgtcga tcgctacttc ggggttgctt ccatgaacgc gctgcgcggc ctatcgggtc
     7681 tagcgcctct gcccaactgc ggcggggatc cggttccggc tgcgggtacc tgccagtcga
     7741 ttggcgactt gaacggacta ttggcccaag ccctcgagat tgcgccggcg gagtcagcag
     7801 attatccgag tccattcccc agcgcgctcg tgagcgcgta cgaccactca ccgttctttg
     7861 cgcccgagta cttcgcggcc gcgccgtgcg aatgtgcaga ctggtgacgg ggcgcctact
     7921 cgtgattgaa ggagccgttg ctcaatacac cagagagtcg cacgcgcagg tcgcatcccc
     7981 gagcttctcg gttcgaaaga acgggctcgt gaggagcgtt acgcgggtgg agtctgggag
     8041 tgggctcggg tagtcctccc aagccggata gccgagcccc agcgcccgca tgaagccggc
     8101 tgtgagcgct cctaccgcat gacagggatt gatgggtgat tcggacttta ctactccgca
     8161 atccgccgcc aacggcaaca tgccggataa ggcacgcaac tccggattgg cgaggagcac
     8221 cacgcccccg tcagggctca gatcctggtc gcagggaacg gcgacctgcg cggcgatcag
     8281 ccagagatga tctggatcgt cgagtcgtgc gcctagcggt tcggcaacct caatcacgtt
     8341 cgagaagaag tcgcgcgggc tcggctccgg tccgcgagcg cgggagagcc aatcggggct
     8401 ggggagggtc tggactatcg gcgccggact gccgagcgtg aaagtcgggc cctgtagctg
     8461 cgtacgcagg aagaactgca tgttgcgggc cgcccgctcg aacgcgacca gtgcgttggg
     8521 atacgcggcg ccagcagttg gcaccaagta catcaaccgc acgtctctgg cattggtgcc
     8581 cgagcgtgag cggtcgtagt tgcagactcc tggcggggcg catgttccgg ccgagcagtg
     8641 tccggatctg cagtctcggt ctgcccagca gacctggccg ttgctgcagc gatgacaatt
     8701 ggaggaaccg ccgcaatcga tgtcagtctc gtcgctgttt cggcgttggt cggtgcaatg
     8761 gctgagcggg cacttgccgt cccagcagcc gccatcgaca caatccgagt ccacgataca
     8821 gcccatccca gaccagcacg tcccgcaatc tggtccccca caatccacgt ccgtctcccg
     8881 accgttctgg atgccatccg cgcagttgct cgattggcag cgctccgcga tacacatccg
     8941 gctggcgcag tcgctggctt gaatgcagtg ctggccctcc gcacagcgtt ggcattcggt
     9001 ctctcccccg cagtccacgt ccgtctcgct gcccgtaact cgagagtccg tgcaggggtc
     9061 tgcgacgcag cgactcagac gacagcgcga agtcgcgcaa tccgcgtccg cgttacactt
     9121 gttctcgaca gggcaagcgt cacagctgcc cccgcagtcc agatcggatt catccccgtt
     9181 cttctgccca tcatggcaag catcgggcgc ctggcaagtt ccatcgaggc accactcgct
     9241 ctcacagtca gcgttgttcg agcaaagccc gccgggcgcg cacggcgagc actccccccc
     9301 gcagtcgagg tcggtctccg caccgtcgaa gcggccgttg tcacaggtcg gggcgggcgc
     9361 acagagactt gccgcgcaaa atcgagagct gcagtcgtcg tcttcttcgc agctcagccc
     9421 gtcatcacat cgagtgcact cgccgactcc accgcagtct acgtcggtct cgaagccatc
     9481 gcgtacgcca ttttcgcatg gcttcgcccg acagatgccc tgctcgcaaa tcgcggattc
     9541 acaatccgat gcctctcggc aacgcttcgt aacctcacag cgcgcgcact cgttgtcacc
     9601 gccgcaatcg gtatcggtct cgacgccgtt cttacgcttg tcgtcgcacc ccgtcgatct
     9661 acacagaccg tggacgcact gccgagtcgc gcagtccgag tcgtcctggc agcggtgatc
     9721 ccccgcgcaa cgctggcaga gattgctccc gccgcaatcg acatccgtct cggagccgtc
     9781 gagcagctcg ttgtcgcagc ccgccgttgg gctcggcgcg gtgaccgggg ctccgcatcc
     9841 gagcaccgta ccgccgagcg caacgagcag ggcgcacccg cgtaatctcc gcgtaggagt
     9901 gaatgttgaa tgccgccgtc gcttcgccgg cgcatgctct cgcgtcgctc gtctcctagg
     9961 gctcgcgtac ggctcgaagc tccggctcgg tggctcgtca gctcgtccga gagagcgggg
    10021 ctccatgtcc tggcgaagtg gtttggaatg agaggtcacg ggcaccgtta tcagcagacg
    10081 gcgcggtgtg cggccgcctt cagtcccaaa gcgtcgcact ttgccccccg gtcgcgcact
    10141 gcgtcgctca taaagttgga tgtcgcagcg ccgttgccgt tgacgtcaat tgcgtgcagc
    10201 cataaatcgc gaatcgatac ttcgagctct cttcaaggtc ggtatgctga gctgcctgct
    10261 gggggcacag aattgcgttc cggacttgga cggcttcgga gtggcaccgc agtgcggcga
    10321 tggcagccgc gatgcggacg agtccgacgt cgactgtggt ggcgtgaacc tgtgcttgcg
    10381 ttgcgacgac ggccgccgct gtactaggga ctcggattgt cagagccagt actgcctcga
    10441 aggcagctgc gcgagcgcac gttgcgcaga cggtgagaag aacgggggcg aggtagacat
    10501 cgactgcggt ggctcgaccc cgtgcaagcg ctgcggcgcc ggccagcgct gtgagcagga
    10561 cggcgattgc ctcaccgggc actgcgaaca gaatgcctgc gccttgtcga gctgtagcaa
    10621 tcagctccaa gacggcgacg agagcgatcc agactgcgga ggtggttgtg ccggttgcgc
    10681 agacggacag cgctgtagcg cggactccga ctgcggcagc cagcactgcg aggcgggccg
    10741 atgctccccg agtaagtgtc acgaccaacg ggtgaacggt ggggagagtg acactgactg
    10801 cggtggcgag accagctgtc cgcgttgcgc actcggggcc gcctgtacac gggatctcga
    10861 ctgtgcaacg gcgacctgcg tcgaccgtgc gtgcgctccg gcgtcctgcg ccaaccagat
    10921 cctcgatggc agtgaggtgg aggtcgattg cggaggtaat gacggctgtt caggttgttt
    10981 ggacggcctc cgctgcgagc gcgaccgcga ctgcacgagc tcctactgct tcgaccacct
    11041 ctgccgcccc gcacattgcg cgagtggatt ggtcgatggc tccgaaacgg accgcgactg
    11101 cggaggagac aacgcgtgct tgcgctgtca actgggacaa gcctgctcga gctatctgga
    11161 ttgcgacagc ggacactgcg agcaccagcg ctgtgtgccc gaaacatgca gtgacggtca
    11221 gctgagtcca ggcgaatccg acgtagattg cggcggagac gaatgcgcgc cgtgcttgga
    11281 tcgaagcatt tgcgcccgcg actccgactg tgtatcgcag cactgctacg acgcgcgctg
    11341 cagcccaacc atggcctgtg aatacaccgg agcgttcggc gagaacgtgc gtcaggtgca
    11401 cctggtctac ctcgtgcccg cggatcgcga ggttcgcgcg aactacatcg ccaatttgga
    11461 gcgatcgatt cggcacatac aggttttcac cgcgcaacag ctgcccgacc atcgaacctt
    11521 cagcctggag tcaccggtgg tgcgcgtcct gaagtccaag cgacccggca gctggttcag
    11581 tacggggtcc ggttccggcg ggtccagctt ggcctttcga gacaacgtga tcagcgaaac
    11641 aggggcgctg ctcggtcgct ccttcggcac ggatccgtgg ctgatatacg ctgacgcgga
    11701 gccggcttgc gatcagaaca cggatgacgc ctggggcaag cagcgagtcg cgggagcaag
    11761 cgtgctgcgc ggcatttccg gcgtcgacaa cgtggatgct tgctctggcg gagcgcgcct
    11821 gcccaccgaa ccgtgcgcgt acactgggcg catggcgcgg tcgctcattc gaggcttgga
    11881 cgcggagtac ctcgactacc tggcgtaccc cacgccgttc acgagctacc tcgcgagctc
    11941 tctcgatcgc agcatctact tcggtgtgga agacgtcggc gatcgctact gcgattgctc
    12001 ggtggttgtg cccgagtagc tccggacggc tgaggtcgaa cggtctcgag cctcagcagt
    12061 cccgtcgctt cgttcgctgg ttcactcgca gtcggtgaac agctgtactc cccgttgggt
    12121 gagagcgtcc aagtactgct tctgctcttg gcaatcgagg tggttgaatc cgatgccgag
    12181 gtagtccccg ggaccgagcg cttccgtcat cgcaagaggc gcgatctggc gaatcttgtt
    12241 catggtcaaa tcgagcgaag aaagcagagg cagcaccgac agcggaccga cctcgctgat
    12301 ttcgttgtcg gtcaggacca gctcggtcaa cccttgtaga tttgcgagtg gcccgagctc
    12361 gctcaaatgc ccacccgaaa acttcaattt ggtgagtgcg gtcagctcga cgagcgggag
    12421 cgtgctgtcg agaccgctgt aggataccgt caactcggtc aagctacgaa gcgcagagat
    12481 cggcgtgaag tcggcgatct cttgaactcc ttcgatggat aagcgtcgca actgcgggag
    12541 cgcggccagg ggggtgatat ccgggatggt atcgaaagcc agctcgagct cggttagtcg
    12601 gtccagagca ctcaaggggc tgatgtccgt gaaccagttc tctcccaagt ttagtttctc
    12661 gagggtcgtg agctgtgcga gaggggcgat atccgtgaaa atgttgtggt agagcgagag
    12721 ctcgcgcagc tgatgtaact ccgagagcgc gcttaggctg tcgatgccgc agtagtccgc
    12781 cgtcagtgaa cgcaggttcg tcagccccag gaatgcgtcc atatttccgg gtgagatccg
    12841 ggaaatagcc agcgtctcga gggaggtcac ggtcgtgagc ggcgtgatat cgaacacgct
    12901 ggagcgagtg agatccagct tcttcagcgc cggcaagtgg gaaatggggg ccagctcgct
    12961 cacgctgctg ccgtcgagca gcagctcctg cagttcgctc gcgttcgaca gcggcgtgag
    13021 gtccatcacc ggatcgtcgg agagctcgag cacggtcagg tctcgcaagc actcgatgcc
    13081 agataggctc gagacgcccg ttcgggcgag ctgcaaagtc gtgagccccg caacgtcctc
    13141 tgcgagcaag gcaccgttgg tgcgcccggt ggctttgcgc acttcgtact ccaaggtcgg
    13201 atccgcgaac gtgagggcgc ccgcacagct gaccacggtt ccgggctggc ctccggtcgt
    13261 gctggtcgtt ccgccgccgg tcggcaattc gcgttctccg ccggaaccgg tctcgcctgc
    13321 ttcggccgat cctccggcgt gatacgtgat attcgaagta ccgccgcgct cgctcgcgtt
    13381 ggtgctagtc aataccccgc tggcgccgcc ggtgtgcagt gtcgtgcttc ccccctgctg
    13441 agtagctcgg gttccgcctt ggggggtgcc gcgaccgccg ctcgatacca gagcgccgcc
    13501 cgctgcatca atgtcatcgg cccctccttc ggcggtgatg tcattgatcg actgaccatg
    13561 acccgcacca ccatccccgc aactcaacgt gagaatcacc gctccgattg aaccaaccca
    13621 aacgcgcatc caaatccttc tctctcgcta gaacgatccc cctagcaaga cgacgctcgg
    13681 gccatatcct ttagaacgga acggcttgcc cgtggcgaaa atggggggtg ggctgccgta
    13741 gctgtgcgca tctgattcag tgacggagag ggagggccgg tggcgatgtc ggatttgggc
    13801 cgaacccaat gagggctgcc ccgtagacga acgcccagac atcgctggcg tcgactccgg
    13861 gtcccagcag gatcggttct cagggcgagc aggcgttgag ttgtgccgcg agggctcggc
    13921 ctttaatcgg atcgatcatc atgacgtaac cgattcttcc gcgcagccaa gcctcgaagt
    13981 gggggcgctg ctcgcgattc tgcgcctgca gtccggtcct acgcgcgttg tgaagtatgg
    14041 cccggagctc ccggagctca tcccgcgcaa cggagagctt gtcattcacg accacacccg
    14101 tgacctcttg gcgccgcgag cgcctttgaa ctcgaccctt tttggggttg agcgcgaagc
    14161 cctcttcttt cacgatgtgc cgaacccgtg cgagcaagag tccaacgttg gctgccgagc
    14221 tctcggcgct gaacgtgaga tcgtccgcgt agcgggtgta ctcgaatccg agtttgcggc
    14281 tagctccgcc gagtctacgg tcgagcttgc gcgcgacgag gttagcgata gttgggctgg
    14341 tgcatgcacc ttgaggtaac gcccgatcgt gtacggcgac ccagtgtttg cgaccctcga
    14401 attcaacgag ggtgcgcgcg gattccgtgc acagcaacgc gagcagcgta gcgaccgccg
    14461 gagaataacc gaggctctcg aacagtccgc gcactcgcgg gaacgtgacg ctcgggaaga
    14521 agtccgccag atcgagattg acgagcacgt gttgcccttg gtgcggggcc gcgttcgaca
    14581 cggtcgaacg acctttcacg aagccgtgcg ccgcctcggt caccggcaat cgactcaata
    14641 tcgtatcgaa gatccaacgc tgggtgcggc gcaactggga cttgggtgag gctatcaggc
    14701 gcgttccacc cgagcgcttg ggaatctcga agtagacgta gtgagtgcgt tcggggctct
    14761 cgtgatgaaa gcaaagccag cgcaacgcgg gaacggtgat tccgagtgct tgcgccaaat
    14821 cggcgggggt ggcgagcagc ggcaggccgg cgcgggtcaa atcctcgatt tgagaccgtc
    14881 gattcccgag ccgatgagaa acgcctcgac ccgcaaatat gatgtcgttg gccttgcggg
    14941 ccgcgcgcgc ttgctgctcg gcctgacgac gtgcggcggc ctcttggcgt ttcagctgct
    15001 ttttttgcgc gtgttcagcg cgcctttgct ggatgacttc ttcagcccgt tgccggccgt
    15061 aagaaacggc caagttgcgg gcgtcccgaa acttcagcca gagatcaccc acgcggtgga
    15121 tctcacgcag ctgcgcgaca ctcaggagac cgcgcagaac caagccacgg tcaatcaacg
    15181 ccgtgcgctc gtcagtctgc ggaggaatgg tgtcgacgcg accgatccag ggcgtcgcga
    15241 aagggttgat cttcagggcg cgtatccgca gctgcgcggc ggataggccc aatatacccg
    15301 gggcttcgaa gcgatcgggg gagttggttt tgggcgtcga gcgcggcgcc tcggctcgag
    15361 cggcgccggg cccgggctcc ggtcgggggc cggacgcgga gagctgactc ggagagcctg
    15421 ccgcagctga cggaggcgcg ggactggtgt ccgggatggg gctgctgaca ccgaaccacg
    15481 acttgattgt gtcccaaagc cccattcatc cctcccattg accccggcag atgcgatgac
    15541 ccacaagact cgatatccct gtagggagac ccggacgttc gacttcgtgc gttggaccgc
    15601 gaccgatcac cgagggactg acgctaccgt aatcgctcga gactctcgat gtcgccttag
    15661 taaggcggta cggtagcgta aggcccaaaa gcttatcggg cgtggggctg ttgatgtgag
    15721 aagcaaacgc aaccacggcg taacaccgca gtatgatggg ctcgtgcgtg cgggtctccc
    15781 tgcagggttc gagttcgagg ggcattgtca ggcatttcct ggggaaatgg aagagaatcc
    15841 ttggctatgc agcctcaacg ccaacaggtg acggcagggg ccgctgcgca gtcgattctt
    15901 gaagaagaag gagcaactgc agcgggcgcg actcagctgc ccatccgaat cgatcaccgc
    15961 ctcgcaggtg gtctcttgaa cccgggctac gtaaagccgt ttgccaagct cgaggggctc
    16021 ctgacgctgg atcaccacgc cggatgcgag gcggatgccc tgcgcctgtt cgggtggcgg
    16081 ggggcccaag gtttgctccc cgagttcctc gggcaaaatc gggcgaaacc gatactgcgc
    16141 ggtcacgaag tcgtacatta cctggccccg cttcgcgagc gtgtgcaggg ccgccatcgt
    16201 ctcggcgcgg gtgagtccgt tggcgagcaa ctccgggagg ctcgcgctgc ggcgttcttc
    16261 gagttggaag cggatttgtt ccatgagcgc ggcgctgggg gacgcgcccg cgaatgctgc
    16321 gtccaagttg gtgccgctgg taaagtcgtt ggcggtccag cccgaaagcg cgagcacgaa
    16381 gctcatctcg cccatgcgca gcacccaaat gctgggcatg cccgtgccga gcaactgaac
    16441 ttccacgctc tcgaccagag gcagcacgcg cgccaaagcc aacaggcgtc gccggcccca
    16501 aactttgatt tcgagcggct tgggcccggt ataaggcgcg ccacgccccg cgatttccgt
    16561 tccccacggc tcgacgacca gggttgctga tttgccagga gtaagaatga acttgagact
    16621 acgcggacca gttttttcgc ggcgacggcg cagcagtgaa agcacggagt agagcgcttc
    16681 cggggccaag ctgacccgtt cggcgggcag catcatggcc gcttgcaact gaccgaatcc
    16741 ccgaagccat gaggcaggca aatcgatctt ctcttcacgc aagccgctgc ccgcgcgaac
    16801 ttcgaagccg ctcgggtcca ccagcaggcg tgtcggtcga tagctacgta aggtttgaaa
    16861 gtgctcgtac aacgcgagcg agtaatcgac attcgtcgtg ccgaggcccg tcgcgccggt
    16921 gtttcggaag gcgccgcggt cgacgctcag gcacccgtaa cacgactcgt ctttagagaa
    16981 gcactcgaag gcgacgacgt cgggcgctac cgtcacgacg gggtcgcacg gcacgagcgc
    17041 acggaacagc gctggatcgt gtgccgagag ctccgcggcc catttgcggc gcgccttcca
    17101 gtacagcccg tgcatcttgc ggaactcagg ctcgagcccc gctggctcgg gctcggtgcc
    17161 gagtcgggcg agctcggctt gcgtctcttg ctcggtgatt gccttacgca gggcggcttc
    17221 gtctgcctcc tgctgagcct tccacgctaa gtaggcgctc ttgtcgcgcg cgtggaactt
    17281 caaatccccg accaccactc catgcagcgc gctgatcgct tcccgaaaac gcagcggact
    17341 cacgagctca gcgtcgaaga aaaccttggg gcgcgccaaa ttcggcgcga agctcagctc
    17401 cgagcctgac gccagctcgc gcagcccgct gtgcccgcta tagcgtaggt cgactctcat
    17461 tgggtaacct gcagagccat cgagagctcc gggaagcttt gctgggtcaa tcgcctcaac
    17521 tccgaatcgc ggagcgcggc gcgggtcagc gtgaccagac ccaagttgcg ctccgccggg
    17581 tgcacgctca tcagcactac ccgcaggagc ggcagcaacg ttggggcttc gctgggggta
    17641 gctgccagcc gctctagaag ctcccgactg acgcgctgtt tgtcccgaga cgcaccggcc
    17701 aaggcacaca gagtggccgc gaacacacgc gcgaggcttt cgggggcagc gtgccggaac
    17761 agggcggcac gctgcagcac gaagctacgt acatccggat aaggagactc gcacagctcc
    17821 gaccacaact cgatgtcttc gccaaaacgc gcggcggcca cccgcagccc ttccccgcga
    17881 acctcgacgt gttgcgcgtc acagagctcc cgggtgtggg atcgctccgc gaacggcgcg
    17941 ctcatcagca ggcccgccac gtagtggacg gcgtctttcc gcacgctcgc taccttcgct
    18001 cgcgcgaggc gtaatagcgc ctcgagctcg agtcgcgtcc gaattggctt ctctttcaac
    18061 caggcaaaac ccagcgctgc aagcggtgca gcgggcgcga gcgcgagctg aatgcacgcg
    18121 tcgagaccca gccgcgtgcc gctcaccaac gccgcggctc gatcgcacac ggccgcgagc
    18181 acctccaggt tctcggcgcc gagcagctcc agccagtctt gaatggtcac tcgctcgagc
    18241 ccaggggcgc gcagaaagag ctcgacgccc agcacctgcg cttcctcgtg ttcggccaga
    18301 accaatgact tgatgtctga aaacggcaga gccgccaagg gcttcgtgta gcccgagcgc
    18361 agcagctcca gcgcgaaagc ccggaccgtg cggctcggcg caccttgcac gagctgcagc
    18421 aagtcagaga acgcgctctc ccaagcggcg gggaaaagag gcattggact cagctccgcg
    18481 agcgtgcgcc catcggcgag cagaagcccg ttcggacgcc gacgcagcac cggagatcgc
    18541 ccgtacaaca catgcatcag gccccacgcg ctgagtagac gccccaccgt gttgaggtgt
    18601 tcatcgcgat acagacgcaa ggccgtcaaa actcccgctc gataggcgag cggtgctcga
    18661 tagccgatct tccgaaaata acgcagcgct cgtcggacga ggtaacgccg cgtgctccga
    18721 gtgaattcgg ccagctccga cttggctttc gtgctgccgg acggagactt ggcacgagcc
    18781 ccggctcggg ccgccggcgc gatgcgctca ggcaccaagg gatcgtttct gagctccaga
    18841 ataggccgtg ccttgccggt ttcgggttcc cacgtatggc ccaccgtgag cagcggtcgc
    18901 cgagtaagac ggtcgaatgc gaccaggaaa tgcgccatca gctcggtgtc gcgcgcgcct
    18961 tcggcgagct tgaacaaacg cttgaccaag cccttgtgct ccgcgcggtg gcacccatcg
    19021 tctacatagg ccaacagtgc ttggcgcgcg aacggccgcg gatcttcgaa ccaaggctcg
    19081 gccagctccg ccaacttgcg cgcgccgtcg aaatgcagca cctcctccaa gaagcgcgcg
    19141 tctcccgctg cgaaatacac atcgattagg agcgctgaac tcgcgcgtgg cggtgtgcat
    19201 ccctcgattt cacgcgccgg gcccgccgcc acgtagccgg ctctgcgttt gcccctcagc
    19261 gagcgagcgt aagcgccgta cgcttcgtcc tccgtcgaat actgtttttc cgtgcgtcgc
    19321 gtcgcgacgt ccgaaccgcc actacaatag agaacggtcg aaccacgcaa ttccgcttcg
    19381 aagaaacgcg tgaattggta accccgaccc gagtacgtga actctaggcg cggcaccggc
    19441 tagagccccc ttccgaagtt gtgtcagcgg tactagcact gcgggtcaac gaggatccgt
    19501 cgggggaacg gtgcagagtc atcgccgggc ctccaccctc cttcgttatc cgatttgcaa
    19561 ttgcggctca aggccgattt ccgttcccat ctcgtcgctg gacgcttgct gggttgagcc
    19621 actgtgtgcc agacaagagc tcgcgtaacg gctcgagcaa ctgcgcaacc gaaagctcga
    19681 gatcgacagc tcagcagttt agtctggcgc gagcatgcgc taggttgagc tagaatgccc
    19741 ggcgtctgac cgtgaccgcc ccagccatac cccagccgat agccatcctg gacgtgcagt
    19801 acttccaaga caaggccagc gcggcctgcg tggtggcgca gcattggtcg gatggagagc
    19861 ccagtgaggt gaagcacagg atcgtttcgc cggtagcacc ctatgaaccg gggcgcttct
    19921 atctgcggga gctgccggcc ttgctcgccg tgctcggcac gctcgagacg ccggtgagcg
    19981 ccatcatcgt cgatagctat gtcgtgctcg acgcggcggg cacgctcggc ctgggcggac
    20041 acctctatcg acacctggac ggcgagcgac ccgtaatagg cgtcgccaag acccgctaca
    20101 agggctcttc cttcgcgttg cccgtgctgc gcggtcagag ccaacgcccg ctcttcgtca
    20161 cggcgctcgg ggtggccgcg gaacgagccg cagagtgggt gaaagccatg cacggtgaat
    20221 atcgaatccc cacgctcctc gcgctcgctg atagtaccgc ccgcgccggc gccaatatct
    20281 gaatctggga ctgtcaaatc tccgtcacgc gaattgtgtg attcctagtc tagagagcga
    20341 ttctgctcgt gtgctcagga cacgccgttc atcctcgccg tcggaccgga gctctcaatc
    20401 gggataaccc cgtgatggcc tacgttgcct cccagagatg ccatatccaa cggcgtgaca
    20461 cgccgtcgtc gagcattccg ttctgagccg ggggccaccg cgtgaacgag cgcgacactc
    20521 gacgcgaacc acccccgcgc agtccctact tcccggggcc ccctctgcac cgcgccggcc
    20581 ctcgcattcg acggcgcagc gagggccggc gcggcgcact gcggtcaggt gggcttcgcg
    20641 acggagctgc tgagagcagg taccgagaag tcgcgcccgt ttcggcggtg gccaatccta
    20701 ttccgaacgt cctgacgcgc agggcgaccg aaagtcagcc atcgagtttc gactcgctcg
    20761 caagcgttcg cctgagtagc caatctgcta cgcacgcagc gtcagaagca caatttgaga
    20821 ggaggccaat cagttcaagc tgcggtcgag cacagcaaat ggatcgcaaa gcgagactga
    20881 cctcgaagca accgtgccgc cggacactcg tacggtcgtg tcgagagacg atcttcaatc
    20941 tcaggagttg ttcgaaccat cgaggcccga acttgctcga ttgatttggc cgtgttagcg
    21001 aaccatgatg ctaacgcgag ccgagttcgt gaatgagttg acgcggacca accgtgatat
    21061 ctacctcggt gcaccgagta aagtgcgaac tcagagctcg atgttggcga cggtcaacgc
    21121 ggtggcggcc ttcgaagcat ccggtaccgg cgcaaacttg ctggcgttga tgcgcacggt
    21181 cggcgcggtt ccccaggtca aaaagaatcg ttataccaac gcactcaacg ctctgtatca
    21241 gagctttccg agccccattt acgttacgat gaacccgctc acgatctcgc tcgcgatggt
    21301 gaacgggatt ggtgttccgc gttcgaacaa cgttccgtcg catcaagtgg atgtgttgct
    21361 ggcgctccag gagttacaca acacggggtc tggtaacgcc cttctcgcgg ccatttgtgc
    21421 ggaggtgaat aagggcggca atggcaatcg agtcgcaatt ggggacgcgg tgggcacgat
    21481 gagcggcggc aacgagtgcc aaatcgtggg taagcccccg gacgattacc aaaccgaact
    21541 cgcggcggcg ctggttgggg atcacagcaa agtcggggcg aagatcgacc aggctctgac
    21601 gaaaatgggt aagcgcggtc aatacgagtg gctgcaagag agaatcaaca acgagccaat
    21661 ttggcagttg caaggggagc ccagcacggt tgggtctgcc agtgtaaacg ggaacaattg
    21721 gatctccgta gaaatgttga agtcctggac cgagcggcga tcgagcttcc cggccccgtt
    21781 gaaggccggt cgcgcttcgg atgcgcaggt agtgctggga gcgatcttgc accccggcgc
    21841 cacgccgggc ccgggcggga gcacgaaagt acgctggagc gccgcaaact tgatggccgc
    21901 gggggcggcg cggccgcctt gcgtcgggct cgctcacgag ttggttcacg ccctccacaa
    21961 tatgcgcggt gaccagccgg ggaacgacaa cgggcatccg actacggcgt tgttcgaata
    22021 catctgtgtc ggtctggggc cctggcatga cgagccgaat accgagaacg cggtgcgcga
    22081 aggcattccc tgcacgcgtc ggccatacta cgcgcccggc ggctgaccgc gcgtgctcgc
    22141 agcaactttt ccggccgtag cactaggaga gtaccaacac gctgatccgc tgaaaggacc
    22201 agtatgcggc cacggtaccc actgcgtaca aggctggtcg gcgggcgtgt gtgtaccaag
    22261 cccgaggaac gggcaatcga agaagggcat aggctagccc aaccatgaac acctgcccga
    22321 cttccacgcc gacattgaag ctgagcagtg cgaatggcag gtgttgcggc gggaggccga
    22381 tgtccttcaa agcaccggcg aagcccagcc cgtgaaccag accgaacaag aaggacacca
    22441 cggcgggcat gcg
//
LOCUS       DASTKU010000961        12351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_410907, whole genome shotgun sequence.
ACCESSION   DASTKU010000961 DASTKU010000000
VERSION     DASTKU010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12351
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410907"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="VFQ61_34100"
     CDS             complement(<1..1559)
                     /locus_tag="VFQ61_34100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959591.1"
                     /translation="MSARSAPSRVTRALGYSLSNTCWQASAAAVTACLYALWVLSDIP
                     THHPSADSHYHFTVAREIGRGVLAPDPARGLPFTVLRDMPVDHYWAFHVLLAPFTWIG
                     DYKWGLRWAASVGFAAVIASLAAFISARRVPNAALWALCAMLFANQDWRYLQLRGAQC
                     MVPLSLLIVELAGFRRASRTRQLALVGTAFIGIWCYHGALVLLPYAAVASCAAHCLDP
                     ALRQLHARVLAVMRDTACVATGLIAGLLVNPYMDARFSTFRFLVFHVTRMLADPQNLY
                     TDVVAEFHGFRGDYFVYMPEWGLLFLATVGTLLWVASRWIVQRPPSRSARVIAAVCIL
                     GMLLTWRAVRMREYSVPVAFALLAVVWSGRSLREPGILSKFGLPAVCALLLLWAMKLR
                     APETRVQIGQSLPTDLFHGAASILERNGEHPILNLAEADYGMLRIETPRVVCVHSLSR
                     YFIYPYRDLYDDVWLIYRDAMRSDVRTRAALERFARRGVRIVATHGNRALDAWALAHP
                     ESLRPVFQSTVG"
     gene            1917..2858
                     /locus_tag="VFQ61_34105"
     CDS             1917..2858
                     /locus_tag="VFQ61_34105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET9959592.1"
                     /translation="MPLETIGTPALWAGFVLFVLCMLAVDLGVFHRKAHAVSLKEAGI
                     WSAVWVAMAGAFNAILYFAFGPERALEFTTGYLIEKALAVDNIFVFVVIFSTFAIPAL
                     YQHRVLFWGVLGALLMRAAFILAGGAFVQRFHWAMYVFGGVLLVTGVKLLIQRNQEPD
                     PEKNVLVRAFRRVVPSTPELHGDSFMIKRAGRRYATPLLLALVAVEVTDLIFAVDSIP
                     AIFAVTEDPFIVFTSNIFAILGLRSMYFLLAGVVNKLAYLKIGLSFVLIFIGFKLLLI
                     DFIKLPIAASLGIIAGILAVSVVASLVKPSVVEQQPS"
     gene            2982..4517
                     /locus_tag="VFQ61_34110"
     CDS             2982..4517
                     /locus_tag="VFQ61_34110"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HET9959593.1"
                     /translation="MSRTRSAWICGWISLGISAACSGQSPTSPGPGSGTGLVGGAGAN
                     TAGVSGSGGAGGSTGGTQPKSGGTAGIAGDSGRGGLAQTGSGGANAGGVQSAGAGGGM
                     LAGQAGASGGSASNGGRGGTSPGGLGGQDGGHNSAGSGGSVERGGAGGVGTSLGGSAG
                     GASTGGASTGGATAQPRFVLGADISSVQESNTTFKDTDGQTKSIFALLKNHGFNYIRL
                     KTFVDPKAPYGYASNSNGCTGLAEAFGDRDHVIAYGKQAKAEGMGFLLDFHYSDTWAD
                     PGNQIIPEAWRGAANITELAAFVKSYTKDVLSKAVQAGARPDMVQVGNEITPGLLAHV
                     PGANTDCWGNNPQSSKVSGSTSNWDNTATLLNAGIEAVREVDPTIQVMLHIENTKDAA
                     GVKRWVDGARSHNVKFDILGLSCYTAFQGASTVWENTFKQMATAYPDLKFAIAEYNPD
                     RTRANLIIKNLPAGKGVGTFFWEPTRSGEWGSAMFTFQGRTATANTQDFAEYDALRPQ
                     LGL"
     gene            complement(4566..4976)
                     /locus_tag="VFQ61_34115"
     CDS             complement(4566..4976)
                     /locus_tag="VFQ61_34115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959594.1"
                     /translation="MGLFERAKPLKDIVQRGASAVRRAAEGLSENGSAIEAKRDAPLA
                     EEGPEPPVVARLVVEIRSDGSRTIARGGLEDVISGQKVAVRADGTTPAQLARSLASTL
                     VTLPVFAARIARSLKRGEELKIDARPDSTRDEHD"
     gene            5122..6588
                     /locus_tag="VFQ61_34120"
     CDS             5122..6588
                     /locus_tag="VFQ61_34120"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959595.1"
                     /translation="MLSRRFLVVSCACWLTHCSVDEKGRTYRGDVSGGSESSSGGRLQ
                     QVGNTSGSSGSGGSAGGMSGAQGGSSGTPDINRSEGGEPSGESGQSNTSGGTTSDAAG
                     VPGVGGADGWGEGGQAGSTGEPPISGLHVLQIAAGSNHTCALLSDQTVKCWGGNGSGE
                     LGDGTDVGALSPVAVSGLSDVTALAAGGSHTCALLTDRTIRCWGDNGRGQLGTGDFVT
                     SLKPRAVVGISDATQIAAGDMHTCALRANQSVRCWGNGEVGQLGDGTSYESKPANSDS
                     PHTPLPVDVSGLSAIIQIALGDQHSCALRRDRFVACWGNNASGELANRSAAEFSPLAT
                     TVWLDPDVTQVAAGDSYTCALLVDHTVSCWGKNDSGQVGDFLGETAPPVLVKGIAEVA
                     QVSTGDSHACVVLTDRTVRCWGWNEWGQLGAVNYGANGAPVIVDLPGPVAQVSAGGYH
                     SCALMSNRKEIYCWGHNQLVGDAVKDTFSPVPIGHVLE"
     gene            complement(6616..7197)
                     /locus_tag="VFQ61_34125"
     CDS             complement(6616..7197)
                     /locus_tag="VFQ61_34125"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET9959596.1"
                     /translation="MSETASKTPANPPPPQPSAQKLFEARTVLIFGEVTTELAESVTA
                     QLLALAATNSEPIRVLLHSPGGHVEAGDTIHDVLRFVAPPVNIIASGWVASAGALIYA
                     AVPRERRVALPNTRFMLHQPLGGVGGPATDVEIEARQIVIMRERLNRIFAEATGQPYE
                     KILSDTDRNYWMSAREAVEYGLVSRIVQNAKEV"
     gene            7311..7910
                     /locus_tag="VFQ61_34130"
     CDS             7311..7910
                     /locus_tag="VFQ61_34130"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9959597.1"
                     /translation="MSAKPTLPKVHVVHCENDGELIVKVTARTTLWVMGEGDLCFELR
                     GTQRLQAADAAVESVRTASAPEPNALRLAAGDARVREAVRLMQAAPEKRWTLRELARA
                     VGVSRAVFAKVFKALIGEAPMAFLTRIRLERARSWLALSDAGLAEIASQSGYANEFAF
                     SRAFRRHFGVAPGRLRRALRVRASSRPVAVGQMSLLCAA"
     gene            8217..8918
                     /locus_tag="VFQ61_34135"
     CDS             8217..8918
                     /locus_tag="VFQ61_34135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959598.1"
                     /translation="MRIYQLAVSIVALGLFACSEEVESTDVRTSGVYPVITVTADGSG
                     SSRVDIELKVGGSSSNTRLDLEGEDNLTVTVGDETRRITESKNNKYTTTFDTDAEGTE
                     FIVAFNRGADDKSAPNSRVSLPAPFDLTLGSSEVSRKDDVLDFTWDKAGGPDMEWHAD
                     GDCIFPESDTTADDGEASIGAGKLDATSSKENESCEVTLSLTRTAKGTADPAFTEGGA
                     VKALQVRSETFQSLP"
     gene            9383..9778
                     /locus_tag="VFQ61_34140"
     CDS             9383..9778
                     /locus_tag="VFQ61_34140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959599.1"
                     /translation="MIVPHPFDIIAILLGLFFALRKSDVRTEELSRHPRANAADFERW
                     QALAFSAYTFGARICFAKVMADVVFLTILRQVTPPLNVQRTIGVSMDLAWMAAVAFTF
                     IRARRARRFAEQHGIDLRRSQRQDAEDQA"
     gene            complement(9810..11156)
                     /gene="lepB"
                     /locus_tag="VFQ61_34145"
     CDS             complement(9810..11156)
                     /gene="lepB"
                     /locus_tag="VFQ61_34145"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET9959600.1"
                     /translation="MPRLERPSRASPIVNAKYLRLLFWPLWFVIVPAVLGYFTVTAIA
                     PGPDYVGTSFLGKILEFVRDQRVPAIIVFFALYEMLLYRVRYHLPLAAYLGVVGRDDI
                     PDNLQRDFEGAVHLLEEADRLLHRHRASIERNIPAAARNELFEAVEALREEVDRERLE
                     PKAFVSAYERASDLVNRRLARWRKGEVREYVESIAVAMLVALTLRAVVIEAFKIPSGS
                     MLPTLQIQDHIFVNKLTYGPMIPFVGKRIFSNLPPKRGDIMVFEYPDPDPTAERQDFI
                     KRAIAFEGDTLEVDGGHPIINGWRVPSCRVGNYEFNEGGGYGLKRGELYIEFLGEYSY
                     LTLYEDVFSSGRQGPYKIKPGETWVLGDNRNNSSDSRAWNHGRGGGVPHDNIKGRAMF
                     VWLSFGTDASVTWDRLFTNVMGHPRLPKEAPIELTRGIERCLSQRPAQTYPPAAKP"
     gene            complement(11215..12273)
                     /locus_tag="VFQ61_34150"
     CDS             complement(11215..12273)
                     /locus_tag="VFQ61_34150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959601.1"
                     /translation="MKLRSAASLALIAASLTACPSRESVGSRSVTILGSGVVNDPKNK
                     SLRFDLLRFGLDSFCGQMQAQGLALKMSDDEPVMGRFYATACNTQVIDEEARKSFVVQ
                     YAGTGFVTSKDGGRLGFSTTGLVEYAPDFLLKDGALYVYFRPRVVDASGFQTLLVESA
                     LANAALKLFGVDANAFGKKVVDAQLRRGLTVIRYNENGETDYGVGFIPLGQKPYHPFS
                     VETEDKRVLLNERTEVHTAQQDFLGPFSVDADDQALYLTAKLEGTAALDVLLIPEATG
                     RSMLNTFTRNAGPVAMSGGALLDEALPRGLLWKRFVPVPKGKYFVVLDHSGALGRTTP
                     SPGPSGRVDVLVLLGDKP"
BASE COUNT         2387 a         3704 c         3980 g         2280 t
ORIGIN      
        1 ccgaccgttg attgaaacac cggtcgcaag ctctccggat gcgcgagtgc ccacgcgtcg
       61 agcgctcggt ttccatgggt ggcgacgata cgcacgcctc gtcgcgcgaa ccgctcgagt
      121 gcggcgcgcg ttctgacgtc cgaacgcatg gcgtcgcggt agatcagcca gacgtcgtcg
      181 tagaggtctc gatagggata gatgaagtag cgtgacagcg agtgcacaca cacgacccgt
      241 ggcgtctcga tgcgcagcat tccatagtcg gcctccgcca gattcagaat agggtgctcg
      301 ccgttccgct ccagaatact ggccgcgccg tgaaatagat cagtcggcaa gctctgccca
      361 atttgaacgc gggtctccgg cgcacgcaat ttcatggccc agagcaggag caacgcgcac
      421 accgctggta acccgaattt cgagagtatt cccggctcgc gcagcgatct ccccgaccac
      481 accaccgcca ggagcgcaaa cgcaacgggc accgaatatt ctcgcatgcg tacggctcgc
      541 cacgtgagca gcatgcccag tatgcaaacc gctgcaatga cacgggcgct gcggcttggg
      601 ggccgctgaa cgatccagcg cgatgctacc caaagcaggg tgccaaccgt cgcgagaaac
      661 aacagacccc attcgggcat gtacacgaag tagtcaccgc gaaatccatg aaactcggcg
      721 accacgtccg tgtacagatt ttgcggatct gcgagcatcc gggtgacgtg gaaaacgagg
      781 aagcgaaagg tagaaaaccg cgcgtccata tacgggttga cgagcagtcc cgcgatcaat
      841 cccgtcgcca cgcacgccgt atcgcgcatc accgcgagca cgcgcgcgtg caactggcgg
      901 agggccgggt cgaggcaatg cgccgcgcag ctcgcgacgg ccgcgtaggg caataggacg
      961 agcgcgccat ggtagcacca aatcccaatg aaagcggtgc ccacgagcgc gagctgccgg
     1021 gttcgagagg cgcgcctaaa gcccgccaat tccacgatca ggaggctgag cgggaccatg
     1081 cactgagcac ctcgcagctg caggtagcgc caatcctgat tcgcgaacag catcgcacac
     1141 agcgcccaga gcgccgcgtt cggtaccctt cgtgcggaga tgaacgccgc caagctcgca
     1201 atgaccgcgg cgaagccaac ggaggcggcc cagcgcagtc cccacttata gtcaccaatc
     1261 caagtgaaag gtgcgagcag gacgtgaaat gcccagtagt gatccaccgg catgtcacgc
     1321 aataccgtga acggaagtcc gcgcgccggg tccggcgcga gcacgccgcg cccaatttcg
     1381 cgagcgacgg tgaaatgata gtggctgtcc gccgagggat ggtgcgtggg gatatcgctc
     1441 aagacccaga gcgcgtacag gcaagcagtc acagcggccg cactcgcctg ccagcaggta
     1501 ttcgaaagcg agtagccgag cgcccgggtg acgcgagacg gagcgctgcg cgcagacatg
     1561 agcgccgagt atgcccgaag cggcaatcga aactcaaaag actcgggtag ccgtcgtggg
     1621 ggaatcacca ccgatgattc atcgcgcacg cgtgcctggg ctacgagcgg acgacacccg
     1681 acgcgggttg ttccgagctg gcgcggctga agtgcgctac cgctgacatc gcccgcgcag
     1741 cgcctgcgtg cgatatccga cacacccaga attcgccgag ctttcccggc ggcgcgctcg
     1801 acatgtaact gagagttaca tgtcgcagac acaactagta attgtctaca tgacgcacga
     1861 aagcaatcat tcggaacgcg cgcggcgcca gccgccgtcc gcaaaggaga ttcatcgtgc
     1921 ccctcgagac catcggaacg cccgccttat gggcgggctt cgtcttgttc gtactctgca
     1981 tgctcgccgt cgatctcggc gtatttcacc gcaaggctca cgcggtcagc ctcaaggaag
     2041 ctggaatctg gagcgcggtg tgggtggcca tggccggcgc attcaacgcc atcttgtact
     2101 tcgcgttcgg cccggagcga gcgctcgagt ttacgaccgg atacctgatc gagaaggctc
     2161 tggccgtcga taacatcttc gtgttcgtcg tcatcttcag cacgttcgcc atcccggcgc
     2221 tttaccaaca tcgggttctg ttctggggcg tgttgggcgc gctgctgatg cgggccgcct
     2281 tcattctggc gggtggcgct ttcgtgcagc ggtttcactg ggccatgtac gtgttcggcg
     2341 gggttttgct ggtcacgggt gtaaagctgc tcattcaacg caatcaagag ccggatcccg
     2401 agaagaacgt tctggtgcgg gccttccgtc gtgtggtgcc ttcgacaccg gagctgcatg
     2461 gcgactcgtt catgatcaaa cgggccgggc gtcgttatgc aacgccgttg ctgctcgctt
     2521 tggtggcggt agaggtgacg gatctgatct tcgcggttga ctcgattccg gcgatctttg
     2581 cggtcacgga ggatcctttc atcgtcttca catcgaacat cttcgccatc ttggggctgc
     2641 gctcgatgta tttcttgctg gccggagtcg taaacaagct tgcgtatttg aagatcgggc
     2701 tctctttcgt gctgatcttc atcggcttca agctgctgtt gatcgacttc atcaagctcc
     2761 cgatcgccgc ttcgctcggc atcattgcgg gaattctcgc ggtttcggtc gtggcttcgc
     2821 tggtaaagcc gtccgtcgtc gaacagcaac cttcctaact tcgaaatcgg caccccgctc
     2881 gaaaacaagc gagcgatgcg ggccgttttg gggtcatgat tgggagcaac ttgccccgac
     2941 agagcgatga cgacgcaacc gaagttcaac ggagaaatgt agtgagtcgt acacggtcag
     3001 cttggatttg cggttggatc agcctgggca tctccgccgc atgctccggt caaagtccca
     3061 cctcacctgg acccggatcg ggaaccggtc tggtcggcgg agcgggcgcc aacactgctg
     3121 gagttagcgg tagcggcgga gccggtggca gcaccggcgg cacacagccg aagagtggcg
     3181 gcacagctgg gatcgcaggt gattcagggc gcggtgggct ggctcaaaca ggttccggcg
     3241 gagcgaacgc gggaggagtg caatccgctg gcgcgggtgg tggaatgctc gcgggccagg
     3301 cgggcgcgtc cggcggaagc gcgagtaacg gaggccgcgg cggcacttcc cccggtggac
     3361 tgggcggtca ggacggtgga cataactcag cgggttccgg cggatccgtg gagagagggg
     3421 gcgctggtgg cgtgggcacg agccttggcg gctctgcagg tggcgcgtct acaggtggcg
     3481 cgtctacagg tggagcaact gcccaaccac ggttcgtttt gggcgcagac atctcgagcg
     3541 tgcaggagtc caacacgacc ttcaaggaca cggatggcca gaccaagagc atcttcgctt
     3601 tgctgaagaa tcacggattc aactacatcc gcttgaagac cttcgtggat ccgaaggcgc
     3661 cgtacgggta cgcctccaac agcaacggct gcacggggct ggccgaggcc ttcggtgatc
     3721 gcgaccacgt gatcgcctac ggtaaacaag ccaaagcgga gggcatgggc ttcctgttgg
     3781 acttccacta cagcgacact tgggcagatc ctggaaatca gatcatccct gaggcgtggc
     3841 ggggcgcggc caacatcacg gaactcgcag ccttcgtaaa gagctacacc aaagatgtgt
     3901 tgagcaaggc agttcaggcg ggtgcgcgcc ccgacatggt acaggtgggt aacgagatca
     3961 ctcccgggtt gctcgcccat gttcccggtg caaacaccga ttgttggggg aacaacccgc
     4021 aatcgtcgaa ggtgtcgggc agtacgtcga actgggacaa cacggcgacc ctcctaaacg
     4081 cggggatcga agcggtgcga gaagtagatc cgaccatcca agtcatgctg cacatcgaga
     4141 acacgaagga tgcggccggg gtgaaacgct gggtcgacgg tgcgcgctca cacaacgtca
     4201 agttcgatat cctcgggctc tcctgctata ccgctttcca aggcgcatcc acggtttggg
     4261 agaacacctt caagcagatg gccaccgcgt atccggactt gaagttcgcg attgccgagt
     4321 acaatccaga ccgcacgcga gccaatctga tcatcaagaa tttgccggcc ggcaagggcg
     4381 ttggcacgtt cttctgggag cccactcgaa gcggtgaatg gggatcggcc atgttcactt
     4441 tccagggtcg aaccgccacc gcgaacactc aagattttgc cgagtacgac gccttgcgtc
     4501 cacagctcgg gttgtagtcg gctcgcgcag agccgcggtc gcgaacgagc cgcaatcgcg
     4561 cgaactcagt cgtgctcgtc ccgggtcgaa tcggggcgag cgtcgatctt caactcttcc
     4621 ccccgcttga gcgaacgcgc aatacgcgcc gcaaacaccg gcagggtgac gagcgtggac
     4681 gcgagtgagc gagctagttg agcgggggtc gtgccatcag cgcgcactgc gaccttctgc
     4741 ccactaatca cgtcctccag gccgcccctc gcgatggtgc gcgagccgtc gctccgaatc
     4801 tccaccacca aacgcgccac gaccggcggc tcgggccctt cctcagccaa cggcgcgtca
     4861 cgcttggcct cgatggcgga accgttctca ctcagtccct ccgccgcgcg gcgcaccgcc
     4921 gacgcacccc tctgcacgat gtccttcagt ggcttggcgc gctcgaacag tcccatgcga
     4981 gtaccttagc attcgggtca gccggcacga gctcgtcgcg ggacccaagt cttcgtcgtc
     5041 cgcgggcagt ggctcttgca gtcgacagcg gaaccaaccc caggtaccgt cgcgcggttc
     5101 atgatcgcca cacaacattc cgtgctatcg cgacgttttc tcgttgtttc ttgtgcctgc
     5161 tggcttaccc actgcagcgt ggacgagaag ggacggactt atcgcggtga cgtcagcggc
     5221 gggagtgaat cgagctccgg agggcgcctc cagcaagttg gaaataccag cggcagctcg
     5281 gggagtgggg gaagcgccgg tggaatgtcg ggcgcacaag ggggctcctc cggcacgccg
     5341 gacatcaacc gaagtgaagg cggagagcca agtggcgagt ctgggcagag caatacctcg
     5401 ggaggaacca cgagcgacgc ggcgggtgtg cctggcgttg gaggcgcaga cgggtggggc
     5461 gaaggtgggc aggcggggag tacgggcgag ccgcccatct ccggcttgca tgtgctgcag
     5521 attgctgccg ggtctaacca cacatgtgcg ctcctaagcg atcagacggt gaagtgttgg
     5581 ggcggcaacg ggtccggtga gctcggcgac ggaacagacg ttggcgcgct gtcacccgtc
     5641 gcagttagcg gactgtccga cgttaccgct ctcgcagccg gagggtctca cacatgcgcg
     5701 ctgctcaccg atcgtacgat ccgctgctgg ggagataacg ggagggggca actaggtacc
     5761 ggcgacttcg tgacctcgct aaagccacgc gccgtagtgg gcatctccga tgcaactcaa
     5821 attgctgctg gtgatatgca cacctgtgcc ctccgggcca atcaatcagt tcggtgctgg
     5881 ggcaacggtg aggttggtca gcttggtgac ggaacctcgt acgagtcaaa accggcgaac
     5941 agtgactcgc cgcacacccc tttgccggtc gatgtttccg gactatctgc gattatccag
     6001 attgcacttg gagatcaaca ctcctgcgcg ctacgacgcg atcgcttcgt tgcgtgctgg
     6061 ggaaacaacg cttccggcga gctggctaac cgcagcgccg ccgagttttc acctcttgca
     6121 acaacggtgt ggctcgatcc agacgttacg caggttgcag cgggtgatag ttacacatgc
     6181 gctctccttg tcgatcatac tgtttcctgc tgggggaaga atgactcggg acaggtagga
     6241 gacttcttgg gagaaaccgc tcctccagtg ctcgtgaaag ggatcgcgga agttgcccag
     6301 gtctcgacgg gagactcaca cgcttgcgtc gtcttgacgg atcgcacggt acgttgttgg
     6361 ggctggaacg aatggggcca gctcggcgca gtcaactacg gcgcaaacgg ggcgcccgtg
     6421 atcgtggatt tgcccggccc tgtcgcacaa gtctcggcgg gtggctatca cagctgcgct
     6481 ctaatgagca atcggaagga aatctattgc tgggggcaca atcagctcgt cggcgacgca
     6541 gtcaaagaca cgtttagtcc cgtgccgatc ggccacgttc tcgaatgaaa gcaacccccc
     6601 ggcgtgcgga cgaattcaaa cctccttcgc attctgaacg atgcgtgaca ccaagccgta
     6661 ttccacggct tcacgggcgc tcatccagta gttgcgatcg gtgtcgctca ggatcttttc
     6721 gtagggttgg cccgtggctt ccgcgaagat gcggttcagt cgctcgcgca tgatgacgat
     6781 ttgacgcgct tcaatctcga cgtcggttgc cggtccaccg acgccgccca acggctggtg
     6841 gagcatgaag cgcgtattgg gcagcgccac tcgtcgttca cggggtacgg ccgcgtagat
     6901 gagcgcgccc gcactcgcga cccagccgct cgcgatgata ttgaccggcg gcgcgacgaa
     6961 gcgaagcaca tcatggatgg tgtccccagc ttcgacgtgc ccgccggggg agtgcaacaa
     7021 gacacgaatc ggctcgctat tggtggcggc gagagccagc aattgggcgg tgacggattc
     7081 ggccagttcg gtggtgacct caccaaagat gaggacggtg cgcgcctcga acagcttttg
     7141 tgcgctaggt tgcggcggcg gaggattggc gggggttttg ctggcggtct cgctcatgtg
     7201 cttcctgtaa gaagacggaa ccgcggcgac catggccgcc gagcttcaac gcatacccgc
     7261 gagtctgtct ggctggactc gcggtgcggc ccctggcatc ctcgttcggc atgagcgcca
     7321 aaccaacttt gcccaaagtt cacgtcgtgc actgcgagaa tgacggtgag ctcatcgtaa
     7381 aggtcacggc gcgtacgacg ctttgggtaa tgggggaggg tgacctgtgt ttcgaactgc
     7441 gcggcacgca acgactgcaa gcggccgatg ctgccgtcga atccgttcga accgcgagcg
     7501 cgcccgaacc caacgcactc cgtctcgccg cgggggacgc gcgagtgcgg gaggcggtgc
     7561 gcttgatgca ggcggcgccc gagaaacgtt ggacgctgcg tgagctcgct cgcgcggtgg
     7621 gggtgtcgcg cgcggtgttc gcgaaggtgt tcaaggcgct gataggcgaa gctcccatgg
     7681 ctttcctcac acggattcgg ctggaacgag cgcgctcgtg gctcgccttg tccgacgcgg
     7741 gattggctga gatcgccagc cagtccggct acgcgaacga gttcgccttc agtcgcgcct
     7801 ttaggcggca ctttggagtg gcgcctgggc gattgcggcg cgcccttcgg gtgcgcgcgt
     7861 cgagccgccc cgttgctgtg ggccaaatgt cgttgctctg cgcggcctga gtttcgaacc
     7921 gcgtgcgtcg gtgtgtcgac gcgaatacac ctcgtgggcg catgccctta cgcgctcggt
     7981 tggctggtct ggatcgctgt cgagttgagc tgggaccgtg aatcgtggaa tgctgtgaac
     8041 ccaatgtcat gctcggcatg cgtagggccc tgcgtgaaag cgcgcgttaa cagaaaaaac
     8101 cgacgaatcg agggcccact cagcgtgttg ctattggcat gacacttgcc gtcaacgacg
     8161 gctcatctgg agctggtctc ggccactccg gaccgactta aggaaacgac gcagccatga
     8221 gaatttacca actagcggtc tcgattgttg ctttgggctt gttcgcttgc agcgaggaag
     8281 tcgagtcgac cgacgtgcgc acttctggtg tgtatcccgt gatcacggtt acggcagatg
     8341 gttccggtag ctcccgagtc gacatcgaac tcaaggtggg cgggtcatca tcgaatacgc
     8401 gcttggactt ggagggcgaa gacaacctta ccgtcaccgt cggggacgag acgaggcgca
     8461 tcacggagag caagaacaac aagtacacca cgaccttcga taccgacgcg gaggggaccg
     8521 agttcatcgt ggcgttcaat cgcggtgccg acgacaagag cgcaccgaac tctcgcgtga
     8581 gcttgcccgc cccattcgat ctgaccctgg gctcgagcga ggtatcccgc aaggacgacg
     8641 tgctcgactt cacatgggac aaggcgggcg gccccgacat ggagtggcac gccgacggcg
     8701 attgcatctt ccccgaatcc gataccaccg ctgatgatgg cgaagcgagc atcggcgccg
     8761 gcaagctcga cgcgacttcg agcaaagaaa acgagagctg cgaggtgacg ctctcgctca
     8821 ctcgtaccgc caagggtacg gccgatcccg ccttcacgga gggtggggcg gtcaaagcac
     8881 tccaagttcg gagcgagacc ttccaatctc tgccgtgacc cgtgcttggg cggaactcaa
     8941 gggagtccgc tcgattcagg gaggacaaaa agacaacggc tcggagccgg gagaagcttc
     9001 gagccgttgg ggagattaat gctggcggcc gaccttcggg ggagggggaa ccgatggcgc
     9061 cgccggcgtt gaggcttagt gcagccgtcg tgccacgctc attcgcgtga gaagccacgg
     9121 cgcttggttg cggaacacac agaaattcag cctcttcagg ttacggagcg attccccgat
     9181 ccacccgctg cgtctctgtc tgcgacaggt gttcagtccg aggatctcga tacgggattg
     9241 gggcgggcag gaaatgcgag ccgagtgtgc tacaggggtt gctgcttggc ggcaggtagc
     9301 tcaactcgcg cacacgctcg cgcggcctgc gcctaagtca gcttcgccta tcccgcagtc
     9361 gctccaacgc acggacccga acatgattgt gccgcatcct ttcgatatca tcgccatcct
     9421 gctcgggctg ttctttgcgt tgcggaagag cgacgttcgg acggaggagc tctcgcgcca
     9481 cccgcgcgcc aacgccgccg atttcgagcg ttggcaggcg ctggctttct cggcttacac
     9541 ctttggggcg aggatctgct ttgccaaggt gatggcggac gtggtgtttc taacgatcct
     9601 gcgccaggtg actccgccgc tcaacgtgca gcgcacgatc ggcgtctcga tggacctggc
     9661 gtggatggcc gccgtggctt tcacgttcat tcgtgcacgt cgggcgcggc gcttcgcgga
     9721 gcagcacggc atcgacctga ggcgctcaca gcgccaggac gcggaagacc aagcttgaac
     9781 gagcacgcgg ccctgagccg gcgtgcggat cagggtttgg ccgcaggcgg gtaggtctga
     9841 gcgggtcgct ggctcaggca gcgctcgatc ccgcgcgtga gttcgatggg ggcctctttg
     9901 ggcaagcgcg ggtgtcccat cacgttggtg aataggcgat cccacgtgac gctcgcgtcg
     9961 gtgccgaagc tcagccacac gaacatggcg cggcccttga tgttgtcgtg cggaacacct
    10021 ccgccacgtc cgtggttcca agcgcgtgag tcgctcgagt tgtttcggtt gtccccgagt
    10081 acccaagtct ctcccggttt gattttgtaa gggccctgac ggcctgagga gaagacgtct
    10141 tcgtagagcg taaggtacga gtactcgccg aggaactcga tatagagctc accgcgcttc
    10201 aagccgtagc cgccgccctc gttgaactcg tagttgccta cgcggcagct cggcacccgc
    10261 cagccgttga tgatggggtg tccaccatcg acctccaacg tatccccttc gaaggcgatg
    10321 gcgcgtttga tgaagtcctg gcgttccgcc gtaggatccg ggtcaggata ctcgaacacc
    10381 atgatgtccc cgcgtttggg gggcaggttc gagaagatcc gcttgccgac gaacgggatc
    10441 atgggcccgt aagtgagctt gttcacgaag atgtgatctt ggatctgcag cgtgggcagc
    10501 atcgatccgc tcgggatctt gaacgcctcg atcacgacgg ctcttagcgt gagcgcgacc
    10561 agcatggcga ccgcgatcga ttccacgtac tcgcggactt caccttttcg ccagcgcgcc
    10621 aaacgccgat tcactagatc ggaggctctc tcgtaggcgc tgacgaacgc cttcggctcc
    10681 agacgttccc ggtcgacttc ctcgcgcaaa gcctccaccg cctcgaacag ctcgttgcgt
    10741 gcagcggcgg ggatgtttcg ctcaatcgaa gcgcggtgac ggtgcagcaa gcgatcggct
    10801 tcttcgagca agtgaacggc gccctcgaag tcgcgctgca aattatcggg aatgtcgtcc
    10861 cgtcccacga ctcccaaata ggcggccagg ggcaggtgat aacgcactcg atacaagagc
    10921 atctcgtaaa gtgcgaagaa gacgatgatg gccgggacac gttgatcacg tacgaattcc
    10981 aggatctttc ccaggaagct cgtgccgacg tagtcggggc cgggtgcgat ggcggtcacc
    11041 gtgaaatagc cgagtacggc tggcacgatc acgaaccaca ggggccagaa tagaagtctg
    11101 agatacttgg cgttcacgat agggctcgcg cggctcggcc gttctagccg gggcatctga
    11161 ttcgagcaat ggctcgcagc gccggctcgt ggttcggcct gcgctgtgtg cgggttacgg
    11221 cttgtctccg agcagaacta ggacatccac gcgacccgac gggcccggcg acggggtcgt
    11281 acggccaagc gctccgctat gatccagcac cacgaagtac ttgcctttgg gaacgggcac
    11341 gaagcgcttc catagcagac cgcgtggcag cgcctcatcg agcagagcgc cgccggacat
    11401 ggcaaccggg ccggcgttac gcgtgaaagt attcagcatg ctgcgcccgg tcgcctcggg
    11461 gatgagcagc acatcgagcg cagccgtgcc ctcgagcttt gccgtcaagt acagggcttg
    11521 gtcgtcggca tcgacgctaa agggacctaa aaaatcttgc tgcgcggtgt gtacctcggt
    11581 gcgctcgttc aaaagaacgc gcttgtcctc ggtctcgacc gaaaacgggt gatacggctt
    11641 ttgcccgagc gggatgaagc cgacgccgta gtccgtctcg ccgttctcgt tgtagcgaat
    11701 cacggtgaga ccgcggcgca gctgagcgtc gactaccttc ttaccgaacg cattggcgtc
    11761 cacgccgaac aacttgagtg ctgcgttggc cagtgctgat tccacgagga gggtttgaaa
    11821 accggaggcg tcgactacgc gggggcggaa ataaacgtag agcgcgccat ccttgagcag
    11881 gaaatcgggt gcgtattcga cgagccccgt ggtcgagaac ccaagacgcc cgccgtcttt
    11941 gctcgtgacg aagccggtgc ctgcgtactg caccacgaag gacttgcgag cctcttcgtc
    12001 gatgacctga gtattgcagg ccgtggcata gaaccgcccc attacgggct cgtcatcgga
    12061 catcttgagc gccagccctt gcgcctgcat ttgtccgcag aagctgtcga gcccgaagcg
    12121 caggaggtcg aaacgcaggc tcttgttttt cgggtcgttc accacccccg agcccaagat
    12181 ggtgaccgaa cgggagccga cgctctcacg ggaagggcac gccgtgagcg atgcagcgat
    12241 caaagccagc gaggcggcgc ttcgaagctt catggggccg ccagcatacc gaagccgaac
    12301 acggctggcg aatgaggctc caaatgcgaa gaacgccggg cccggtgggg c
//
LOCUS       DASTKU010000962         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1306786, whole genome shotgun sequence.
ACCESSION   DASTKU010000962 DASTKU010000000
VERSION     DASTKU010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306786"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="VFQ61_34155"
     CDS             <1..1316
                     /locus_tag="VFQ61_34155"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET9959602.1"
                     /translation="CRGPTTSRDLANALGLTPSRVEEALRRLELEGFVMRCRSESAVS
                     PPRYVARRLLARIHAYTVQRLRREIEPVSQRDFMRFLLEWQHVTPAARLRGPQAVLRV
                     IDQLQGFEAAAGAWEPELLRARIADYDPKWLDELCWSGELVWGRLAPARPARDEAQQR
                     SQVTRSSLITLLRRADLDWLLAAARGSTAAREPVTHETARILDELSRRGALFSSELAH
                     ASGYSRRDVEDALWEGVALGRLTCDGFHAVRALVGASQEPHTAVPRLPIGFRKPGSSL
                     PRGDGRWALLPDPTPSMDPDELAEAVAGQLLHRWGVLTIELIRQETTSLSIRDLISAL
                     RRLEARGLVRGGRFVSGFSGEQYALTEALETLRSVRKQALAEVEVRLSACDPLNLVGV
                     VVPGARVPRLRGNWVLYRDGLPIDDRTGFAENGDAAHATRREYEL"
     gene            complement(1340..2032)
                     /locus_tag="VFQ61_34160"
     CDS             complement(1340..2032)
                     /locus_tag="VFQ61_34160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET9959603.1"
                     /translation="MLKIRRASERGHANHGWLDSFHSFSFADYYDPNHMGFRNLRVIN
                     EDRVAPGRGFGTHGHRDMEIVSYVLEGALEHKDSLGTGSVIRPGDVQRMSAGTGVTHS
                     EFNASRDEEVHFLQIWLLPGGRGLKPSYEQKTFEPATGALRLIVSPTGEGESLTIHTD
                     ARIYAASLTAGQSAQHTVATGRHAWVQVARGSLVLNGRELSVGDGAAISDESSLSLEA
                     RSDAEVLVFDLP"
     gene            complement(2205..>3028)
                     /locus_tag="VFQ61_34165"
     CDS             complement(2205..>3028)
                     /locus_tag="VFQ61_34165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959604.1"
                     /translation="KYSKGTLTDLGYYLGDSRAEALAANDGGSVIVGWSGAEGSTTST
                     KNAVTWTGAGTKVLPKLAGAVLCSATDVSKDGSVIVGECDGIPVRWKAGVVESLPAPE
                     GLFALETLVSGNGTTVVSTLSKYPYIRPAVWDEAQRAFEVVPNFANYTQCVLKALTHD
                     GEAGVGTCASASVGTAVRWSSGLGLTGLPSCREDVSVYQSYDISGDGSVIVGDCVWSG
                     NEAIPNILTRLPSSVTGDDAYSSSTTRHVSADGKRLVISMMNAKFDTDWALVRLD"
BASE COUNT          570 a          977 c          926 g          555 t
ORIGIN      
        1 agtgccgcgg cccaaccacg agccgcgatc tggccaatgc gttgggacta actccaagtc
       61 gtgtcgagga agcgctacgg cgactcgagc tcgagggctt cgtcatgcgt tgtcgttcgg
      121 aatcggccgt atcgccgcct cgctacgttg cccgacgctt actggcgcgg atccacgctt
      181 ataccgtgca gcggctacgt cgcgagattg agccggtgtc tcagcgtgat ttcatgcgct
      241 tcttgctcga atggcagcac gtgaccccgg ccgcgcgcct gcgtgggccg caagcggtgc
      301 tccgtgtcat cgaccaactg cagggattcg aggcggccgc gggagcttgg gagccggagc
      361 tgctacgagc gcgaatcgcg gattacgatc ccaagtggct cgatgagctc tgttggtccg
      421 gcgaactcgt ctggggccgc ttggcgccgg cgcggccggc gcgagacgag gctcagcagc
      481 gcagccaagt gacccgctcc agtttgatta ccctactgcg tcgcgcggat ctggactggc
      541 tactggcggc ggcacgcggg tcgaccgcgg caagagagcc ggttacccac gagaccgcac
      601 gcattttgga cgagctatca cggcggggcg cccttttcag cagcgagctc gcacacgcga
      661 gcggctactc gaggcgggac gtcgaagacg cgctctggga aggcgtcgca ctcggtcgac
      721 tcacatgtga cggcttccac gccgtgcgtg ccttggtcgg cgcctcccaa gagccgcaca
      781 cggccgtacc acggctaccg attgggttcc gtaaacccgg ctcttccctg ccgcgagggg
      841 acgggcgctg ggcgctcctg ccagatccca ccccgtccat ggatccagac gagctcgcgg
      901 aggcggtcgc ggggcagctc ctccatcgat ggggcgtatt gaccatcgag ttgatccggc
      961 aagagacaac cagcttatcg attcgagatc tgatatccgc cttgcgccga ctcgaagccc
     1021 gagggctcgt gcgcggcggg cgtttcgtgt ctgggttttc gggggaacaa tatgcgttga
     1081 cagaggcact cgagacgctg cgcagcgtac ggaagcaagc gctcgccgaa gtcgaggtgc
     1141 gactctcggc atgtgatccg ttgaatctgg tcggcgtcgt cgttccgggc gcccgagtgc
     1201 cgcgcctgcg gggcaattgg gtgctgtatc gcgatggtct tccgatcgac gatcgcacgg
     1261 gttttgccga gaacggcgac gcggcccacg cgacacgccg agagtacgaa ctctgagcag
     1321 caccttcggt tgcatgccat cagggcaagt cgaacacgag cacctcggcg tccgagcgcg
     1381 cctcgagcga gagcgagctc tcatccgaaa tcgcggcacc atcaccaaca ctgagctccc
     1441 gaccattcag aaccaacgaa ccgcgcgcca cctgaaccca ggcatgtcgc ccggtcgcca
     1501 cagtgtgctg tgcggattgc ccggccgtca gcgaagccgc gtatatccgt gcgtcggtat
     1561 gaatggtcaa actctcaccc tcgccggtcg gagacacgat caaacgcagc gcccccgtag
     1621 ccggctcgaa ggtcttctgc tcgtagctcg gcttcaatcc gcgcccgccc ggcaacagcc
     1681 agatttgcag gaaatgcacc tcttcgtctc gagaggcgtt gaactcgcta tgagtcacac
     1741 cggtcccggc gctcatgcgc tgtacatcac cggggcggat cactgaaccg gtgccgagtg
     1801 aatccttgtg ctccagggcg ccctcgagca cataagacac gatctccatg tcccggtggc
     1861 cgtgcgtccc gaagccccgc ccgggcgcca cgcgatcctc attgatcacg cgcagattgc
     1921 ggaagcccat gtgatttggg tcgtagtagt cagcgaacga aaagctgtga aacgaatcca
     1981 gccaaccgtg attggcgtgg ccacgttcgg aggcgcgtct gatcttcagc atgggattct
     2041 tctcctctgc gggaagacag aacgtaagca tcccctctcc cgggagcaag caatggtgcc
     2101 ggcgcagcgc tgagacacac tgtgcaagcc acggaacaat cgcgaggccg cactcgacgc
     2161 gcggccccgc ggactctaac cggcaaagtg ctggaatgaa cgatttagtc cagcctgacg
     2221 agagcccagt cggtatcgaa tttggcattc atcatactga tgaccaaacg cttcccgtcc
     2281 gcgctgacat gtcgagtcgt ggaggaactg taagcatcat ccccggtcac cgaactcggc
     2341 agtcgcgtca ggatgttggg aattgcctca ttgcctgacc agacgcagtc gccgacgatg
     2401 acgctgccgt ccccgctgat gtcgtaagac tggtagacgg acacgtcttc tctgcagctt
     2461 ggaagcccgg tcaaccctaa gcccgaagac caacgtacgg cagttccgac actcgccgaa
     2521 gcgcaagttc cgacaccggc ttccccatcg tgggtcaatg ctttgaggac gcactgagta
     2581 tagttcgcga aattggggac cacctcgaac gcgcgttgcg cctcatccca aacggcggga
     2641 cgtatgtacg gatacttgga gagggtgctg acaaccgttg ttccgttgcc actgactaga
     2701 gtttctaagg cgaagagacc ctcgggagcg ggaagcgact cgaccactcc ggccttccaa
     2761 cgcaccggga ttccgtcgca ctcgcccaca atgacgctgc cgtccttgct gacgtcggtg
     2821 gccgaacaca acactgcgcc tgctaactta ggcaaaacct tggtcccggc tccagtccag
     2881 gtgacagcgt tcttcgtgct ggttgtagaa ccctccgcgc cactccaccc tacgattacg
     2941 ctcccgccat cgttcgcggc cagtgcctcg gcgcgcgagt cgccaaggta gtagcccaag
     3001 tccgtcagtg tccccttcga gtatttgg
//
LOCUS       DASTKU010000963        12011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1306835, whole genome shotgun sequence.
ACCESSION   DASTKU010000963 DASTKU010000000
VERSION     DASTKU010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12011
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306835"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFQ61_34170"
     CDS             <1..600
                     /locus_tag="VFQ61_34170"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR00034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET9959605.1"
                     /translation="RTTESQTHREMASGLSMPVGFKNGTDGGLSVAINAMIAAASPHS
                     FLGVDAEGRVGVVRTSGNPYTHLVLRGGSRGPNFGSAEVAQATQALLKAKVNPRVLVD
                     ASHDNSGKDPARQPFVVADVGAQIQAGAGNILGVMIESNIVAGRQELGGVEGLRYGQS
                     ITDGCIDFATTERVLEGLARDAAGHKLSRAVNGAAASAG"
     gene            735..1943
                     /gene="nosP"
                     /locus_tag="VFQ61_34175"
     CDS             735..1943
                     /gene="nosP"
                     /locus_tag="VFQ61_34175"
                     /inference="COORDINATES: protein motif:HMM:NF041558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035991 - nitric oxide sensor activity
                     [Evidence IEA];
                     GO_process: GO:0046209 - nitric oxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing protein NosP"
                     /protein_id="HET9959606.1"
                     /translation="MSQTAARESSSFLAASGACASDVAPGIRRAGVRESDPAKAARAL
                     ADAILQPDTRFAVFYCSPKYDLDQLGAALAQEFEGVDLIGCTTAGEISPLGYTCDTLV
                     GASLAGPEFTVVTSRIDELSRFEVANGEALASSMLEALASRGGPGSATASTFAFLLID
                     GLSQQEEAIVSSIYQGLGNVPLFGGSAADETRFQRTFVFHQGKFRADAALVTLVRTER
                     PCIVFKTQHFVSSEQRMVVTRASPERRVVTEINGAPAGREYARMVGLEVDKLTPLIFS
                     TYPVVVRVGGAYFVRSIQKVNDDESLTFFCAIDEGIVLTVARGIDFVANLEHAFADVS
                     RKIGAPELVLGCDCVLRNIEVSQRNLRDSVSRIFRDNNVIGFATYGEQFNAMHVNQTF
                     TGVALGRRGR"
     gene            1924..4245
                     /locus_tag="VFQ61_34180"
     CDS             1924..4245
                     /locus_tag="VFQ61_34180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9959607.1"
                     /translation="MEDEGVSSPAADESRESGATSSPAVTSELARLRKRCAELEEELT
                     RQEKITRALANRVERGMDMQGGAFSLFQAATALERKVQLRTEALKDAMGELERSNGEL
                     KLAKEAADAANRAKSEFLANMSHEIRTPMNGVMGMTELLLATDLSQRQRKLAENIQRS
                     ADSLLTIIDNILDFSKIEAGKLELELATFDLFDAVEETLELLSERAHRKGLELVCQLP
                     NSERLELRGDAGRLRQILTNLVANAIKFTEKGSVVVRVFPERSPNSSEPAGAAADADE
                     LSVRFEVEDTGIGLAAAASDKIFEAFRQADGSTTRRYGGTGLGLAIAKQLATLLGGSI
                     GVVSELGKGSTFWFTARFGRTPKAQTMRSAARCAGLQALVVVRNAAQRAALAQLLQSI
                     GVTCETAKDPLGALTALYRMRAEQRAPRVLLIDQLQPEGERAMLIQACEQLECPPSGI
                     VLLTSFGQTPDSSLEYQRTFFVSKPVRRARLIEAIECAAELRQQYGQSERPVSSLSLR
                     APDSLGLKVLVAEDNPINLGVTVGMLELLGCKVDAVENGKLALVALSARQYDVILMDC
                     QMPELDGFQATRAIREREESTQGHVRIVALTANALAQDRERCLAAGMDDFLSKPFSKN
                     QLYEVLCRGDVAKSQDLENAVYSNAPLATTSGPPIDPAVFANLEMLERSGRPGFVDSL
                     IATFVDHGILLSNRLEAAGKRGDLVEVRAIAHQLISSSGSLGASSLARTASRLEEIAL
                     RGELEPARKSIERLKLQFELVVSALRQRQRRPR"
     gene            4622..5872
                     /locus_tag="VFQ61_34185"
     CDS             4622..5872
                     /locus_tag="VFQ61_34185"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9959608.1"
                     /translation="MMQAVQGKVDSRSMREINRSIVLDIIRRGGRVSRTDLARRSALT
                     KPTVSAIVEELLARGIVQEVGYGKALGSGGRRARLLEFNDASAAYLGLRYSATTTQIG
                     IADARGTLRAVRELSHPHGDAHVSADPEARILETLSCLDEVLNEASVPRDRLQSVGVT
                     VSGLVSQADGIVENAPNLGWHKVPVKDIVSRALGLPVAISNDVHGSALAEARCGAARG
                     ARGFVWVYVGTGVGSGIVHGGELFHGCSGYSGEIGHCPVLVEGPECSCGRRGCLEAVA
                     SGWAIARDATRAVQSGERTALADVAGPLDARTVSALAVEGDEVARRILTQVGTYVGIG
                     ISTLVNLFDPELIVLGGKVMLGGDWIVQAARESLAKHTVRRDEVRIVTSSLGDQVGLL
                     GALMNAMDLSVRSYRVVATGERVA"
     gene            complement(5940..7289)
                     /locus_tag="VFQ61_34190"
     CDS             complement(5940..7289)
                     /locus_tag="VFQ61_34190"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: protein motif:HMM:NF009789.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HET9959609.1"
                     /translation="MRTREPSPSHTESAEEYAFDGLIGPTHHHAGLSPGNLQSQSHAG
                     DIGHPRAAALQGLEKLRRVSALGGRKAVFPPQERPYLPVLRRLGFRGSDRDVLEAAYR
                     HDPLLLSAASSASSMWAANAATVAPSSDTSDGRVHFVPANLNSMLHRSFEAPATSRML
                     QRLFQDEQQFCVHEPLPSQAALADEGAANHTRLTTLQGYAHVLFWGRAAGVAALPQRF
                     PARQTRDASAALARLLRVEESALLWQQDPEGIDAGAFHSDVLCVGAGSLLLLHERAVV
                     HPTRFLSELDARLGPEFKAVLATEQEFPVVDAVASYAFNCELIRIADGYLLVAPLETQ
                     GNTAARNFVESLEARVGKRVQLCFVDVNASMNNGGGPACLRLRVQLTERERATIRARV
                     FYEAGLDAELVHWVGRHYRDRLRIEDLRDPGLLEESRRALDELTGILGLGSIYEFQK"
     gene            7734..8738
                     /locus_tag="VFQ61_34195"
     CDS             7734..8738
                     /locus_tag="VFQ61_34195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9959610.1"
                     /translation="MSKTFVKGVRDWLLLAGLGCSLGLPVTWCSSAHAEDQKELARAR
                     VQFQQAIELEQAGNYASAVKLFRAVGQVRMTPQVRFHIALCEEKLGRLVAALGGLELA
                     LSEADSVGPEFRSEVEANVSRLRERIPKLVIQRGTGAEAATIELDSVVLGQGSVGVDV
                     PLDPGPHPVTARAPGYQPFESTVTLVEGEKKVLEIVLEPSPVVTEPPVASGPAPVRAP
                     ERKPNIVPFVVGGVGAATLVASGVFFALRQSTLSSLQSACPVRLECSQSEESKYDRLK
                     IYHYTALTTLGVGLAAVGTGAALFAIDLNARKESAVGLKIVPRFSPTFAGASAQLRFY
                     "
     gene            complement(8862..10424)
                     /locus_tag="VFQ61_34200"
     CDS             complement(8862..10424)
                     /locus_tag="VFQ61_34200"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9959611.1"
                     /translation="MTSATDIGPAVISGIVAPGHVLGRYELLMPIAAGGMAMVWAARL
                     KGTRGFQKIVAVKTMLPKLSEDAQFEQMFLDEASLALQIRHPHVVDILDLGEQNGVLF
                     LVMEWIDGVPLNQLMKASKGAGGIPLSIAVRIVMQASAGLHAAHELRDPQGQLVGLVH
                     RDVSPQNILVTYDGVTKVVDFGVAKATAIMGSGTSPGQLKGKVGYMAPEQIDGDDFDR
                     RADIFALGIVLYALTTGKHPFRRESDAATMYNICSPQAALPPTRVMPDYPRALEAVVL
                     RALAKNPADRYQTANEFLRDLDRALPTDQRALTDDDVAQFVRKLVGDKLQERRSTLSD
                     ALVRADQNASLLPKLTDAPSHPSNGSISNHGITLAGGDTNATTELSGFARRRRNRTLL
                     MGAALVSASAALVLAVVRWVSTPDKPPSTALPTQSVAPAAQPTTGPGKTETDRSGAER
                     SNPDAAPSSSAGEDAAPLNGGSQRDNAATPDSSASKGARPPTPRPAGSTPPKQPTTQP
                     RKKNDWRIDPGF"
     gene            11057..>12011
                     /locus_tag="VFQ61_34205"
     CDS             11057..>12011
                     /locus_tag="VFQ61_34205"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9959612.1"
                     /translation="MSRLPALLTRVIPELYVARQGTGGSFLDVLSGVAATRPDAVALW
                     MGERRITYAQLALEVDVQAARFLRAGMRPGQRAALCGSNSLEYVVALLAAARLGVPVA
                     LPDFELSSELLGEALRRAGAEFVLCDAASEARVQSVTSVPRCTIGSIETAPQSASERE
                     PSDQRLLREIELVGARPGWNSDFAYVFTSGTTGKSKPCRVTHGRALLASSAFGRLVMR
                     LRPDDVLYCPLPLCHSSALLLGLCACLRTGATLVLRERFSARHFLADVRRTRATVLLY
                     VGELGRALVQLPESPLDREHALRLAVGNGMHESVWEGLQRRF"
BASE COUNT         2062 a         3654 c         3978 g         2317 t
ORIGIN      
        1 cgtacgaccg agagccagac tcatcgcgag atggccagcg gtctgtccat gcctgtgggt
       61 ttcaagaacg gcaccgacgg tggtttgagt gtggcgatca atgcgatgat cgcggcggcc
      121 agcccgcata gtttcctggg cgtagatgcg gaaggtcgcg tcggcgtagt gcgaacctcc
      181 ggcaatccct atacgcacct cgttctgcgt ggtggcagtc gcggcccgaa cttcgggagc
      241 gccgaagtag cgcaggccac tcaggcgctg ttgaaggcga aggtcaaccc gcgcgtactc
      301 gtggacgcca gccacgacaa cagcggtaaa gatccggcgc gacagccctt cgtcgtggcc
      361 gatgtgggcg cgcagatcca agccggtgcg ggcaatatcc tgggtgtcat gatcgagagc
      421 aatatcgttg ctgggcgcca agagctcggg ggcgtagagg gcttgcgtta cggtcagagc
      481 attacggacg gttgtatcga cttcgcgacc accgagcgcg tgctcgaagg cttggcgcga
      541 gacgcagcgg gtcacaagct ctcgcgcgcc gtcaacggcg cggcggccag cgcgggctga
      601 gcacggcctt ttcgatctgc gcgtgctcgc ggggagcacg tgtttgaggg ttcgacaggc
      661 ggctgcgacg tggcgctgcc acaggctatt aacactgtgg cagcgtgcgc cgttgcgcgg
      721 ggcggagtcg cgatttgtct caaactgctg cgcgcgaatc gtcatcgttc ttggccgcgt
      781 cgggagcctg cgcgagcgac gtcgcgccgg gtattcgccg cgcaggagtt cgtgagagcg
      841 atcctgcgaa agcggcccgg gctctcgcgg acgcgatctt gcagcccgac acgcgattcg
      901 cggtgttcta ttgttctccg aagtacgacc tagatcagct cggggccgcg ctggcccagg
      961 agttcgaagg ggtcgatctg atcggttgta cgacggcggg cgagatttcc ccgctcggat
     1021 acacgtgcga cactctcgtg ggcgcgagtc tcgccgggcc ggagttcacg gtggtcacga
     1081 gtcgcatcga tgaactctcg cgcttcgagg tcgcgaacgg ggaagctctg gcaagctcga
     1141 tgctggaggc cctggcgagt cgtggcggcc cgggctcggc gaccgcgagt acgttcgcat
     1201 tcttgctgat cgacggcctc tctcagcagg aagaggcaat cgtcagctcg atatatcagg
     1261 gcctcggcaa cgtccccttg tttggtggtt cggcggcgga cgagacgcgc ttccaacgca
     1321 ccttcgtgtt ccaccagggg aagttccgag cggatgcggc gttggtcacg ctggttcgaa
     1381 ccgagcggcc gtgcatcgtc ttcaagacac agcacttcgt gagctccgag cagcgcatgg
     1441 tggtgacgcg cgcgtcaccc gagcggcgag tcgtgaccga gatcaacggc gctccggcgg
     1501 ggcgagagta cgctcgcatg gtcggcttgg aagtggacaa gctcaccccg ctgatcttct
     1561 ccacctatcc cgtggtggtg cgggtgggcg gcgcctactt cgtgcggtca attcagaagg
     1621 tcaatgatga cgagagtctc acgttcttct gcgcgatcga cgaaggtatc gtgctcacgg
     1681 tggcccgagg catcgatttc gtcgccaatc tggagcatgc gttcgcggac gtgagtcgca
     1741 agattggcgc gcccgagctc gtgctcggtt gcgactgtgt gcttcgcaac atcgaggtga
     1801 gccaacgcaa cctgcgcgat tcggtgagcc gtattttccg tgacaacaac gtgattggct
     1861 ttgccacgta cggcgaacaa ttcaacgcca tgcacgtgaa ccagaccttc acgggagttg
     1921 cccttggaag acgagggcgt tagctcacct gcggccgatg aatcccgcga atctggcgcg
     1981 accagctcgc cggcagtgac ttcggagctc gcacgcctgc gtaaacggtg cgcggagctg
     2041 gaagaagagc tcacccgcca agaaaagatc acccgcgcgc tcgcgaaccg tgtcgagcga
     2101 ggcatggaca tgcaaggcgg ggcgttctcc ttgttccagg cggcgaccgc gctcgaacgc
     2161 aaggtacagc tgcgcaccga ggcgctcaaa gatgcgatgg gcgagctcga gcgctcgaac
     2221 ggcgagttga agctcgccaa ggaggcggcc gacgcggcca accgcgccaa gtcggagttc
     2281 ttggccaaca tgagccacga gatccgcacg cccatgaatg gcgtgatggg tatgaccgag
     2341 ctgctgctcg caaccgacct gagccagcgt cagcgcaagc tcgccgagaa catccagcgc
     2401 tcagccgact cgctgctcac gatcatcgac aatattctgg atttctccaa gatcgaggcc
     2461 ggcaagctgg agctcgagct cgcgaccttc gatttgttcg acgcggtcga ggaaacgctc
     2521 gagttgctat ccgagcgagc ccatcgcaag ggactcgaac tcgtctgtca gttaccgaat
     2581 tcggagcggc tcgagctgcg cggagatgcc ggtcggctgc ggcaaattct caccaacttg
     2641 gtggcaaacg cgatcaagtt caccgaaaag ggttcggtgg tggtgcgggt cttcccggag
     2701 cgctcgccga attcatccga acccgcaggc gctgctgctg acgcggacga gctgtcagtc
     2761 cgcttcgagg tcgaggacac cggaattggc ctggcggctg cggctagcga caaaatcttc
     2821 gaagccttcc gacaggcgga tggatccact actcgccgct atggtggcac ggggctcgga
     2881 ctcgcgatcg ccaaacaact tgcgaccttg ctgggcggct cgattggtgt ggtgagcgaa
     2941 ctcggcaaag gatccacctt ctggtttacg gcgcgtttcg ggcgaacccc caaagctcaa
     3001 accatgcgct cggcggcgcg ctgcgcgggg ctccaagcgt tggtggtagt gcggaacgca
     3061 gcacaacgcg cggccctcgc tcagctactc caatccatcg gagttacgtg tgaaaccgcc
     3121 aaagatccgc tcggggccct gaccgcactg taccgcatgc gcgccgaaca gcgcgcgccc
     3181 cgcgtgttgc tgatcgatca gctgcagccc gagggcgagc gcgcgatgct gatccaggcg
     3241 tgtgaacagc tggaatgtcc accgagcggc atcgtgctcc tgacctcgtt cggtcagacc
     3301 ccggactctt cgctcgagta ccaacgcacc ttcttcgtta gcaaacccgt acgaagagcc
     3361 cgcttgatcg aagccatcga gtgcgcggcc gagctccgcc agcaatacgg ccaaagcgag
     3421 cgaccggtgt cgtcattgtc actgcgcgcg ccggactcgc tcgggttgaa ggtattggtc
     3481 gccgaagaca accccatcaa cctcggcgtt accgtcggaa tgctcgagct gttgggttgc
     3541 aaggtggacg cggtcgagaa cggcaagctc gcgctggtgg cgctctccgc gcgtcagtac
     3601 gacgtcattt tgatggactg ccagatgccg gagctggacg ggtttcaggc gacccgcgcg
     3661 atccgagaac gcgaagagag cacgcagggg cacgtgcgca tcgtcgcgct cacggccaat
     3721 gcgctcgcgc aggaccgcga acgctgccta gccgcgggca tggacgactt cctgagcaag
     3781 ccgttctcga agaatcaact gtacgaagtg ctgtgccgcg gggatgtggc gaagagccag
     3841 gatctcgaaa acgcagtcta cagcaacgcc ccgcttgcca cgacatccgg tccaccgatt
     3901 gacccggccg tgttcgccaa tctcgagatg ctcgagcgct ccggacgacc cggtttcgtc
     3961 gattctctga tcgcgacgtt cgtcgatcac gggatactgc tctcgaatcg gctcgaagcc
     4021 gctggtaaac gcggtgattt ggtcgaagtc agagccatcg cgcaccagct gatctcgagc
     4081 agcggctcgc tgggggcgtc gtcgttggcg cgtactgcga gccgcctcga ggagatcgca
     4141 ctgcgaggcg agctcgagcc cgctcgcaaa tccatcgagc gcttgaagct gcagttcgag
     4201 ctcgtcgtga gtgcgctgcg ccagcgtcaa cgacgaccgc gctgagcctg aattctgcgc
     4261 cgcttggcct ggacttcgcg cctcctagtc cgaatcgagc aatcgtctcc gcgtttggcg
     4321 aaaattcggg ctgggttgct cgtcggattg ctgcaatgtt tcgccagata tggaatctgg
     4381 cgacgcgagg cccaaagtgc gcgagctcgg ggttcgaaac cttgtggctt ggccggccag
     4441 gtatcatttt cgaccgaacg actcgatttt agttcgcgtt tgctgctaaa aatgcgccgt
     4501 tctaatgatt tgagcaggga gcctcttcgg aagtctggcc gaactttatg agattggttc
     4561 ggagctgacc tttactcgcc ctctccggcc actacagaaa ggctatccct cgaggtccaa
     4621 tatgatgcaa gccgttcagg gaaaagtcga tagtcgctcg atgcgggaga tcaatcgatc
     4681 gattgttctc gatatcattc ggcggggagg ccgtgtttcg cgcacggatc tcgcccgaag
     4741 atccgcgctg acgaagccga ccgtgtcggc gattgtcgaa gagcttctcg cgcgaggtat
     4801 cgtgcaggag gtggggtacg gcaaggcgct cggtagcggc ggcaggcgcg ctcgcttgct
     4861 cgagttcaat gacgcatcag cggcttattt gggtttgcgc tactcggcca cgaccacgca
     4921 aatcggtata gccgatgctc gaggcacgct gcgcgcggtt cgtgagctct cgcacccgca
     4981 cggggatgcg cacgtttccg cagacccaga agctcgcatc ctcgagacgt tgagttgcct
     5041 agacgaggtc ttgaacgaag cttccgtacc acgggatcgc ctgcaatcgg ttggcgttac
     5101 ggtctccggc ttggtctccc aagctgacgg tatcgtcgag aacgcaccca acttgggttg
     5161 gcacaaggtg ccagtcaaag atatcgtatc tcgcgcgctc ggtttaccag tcgcgatctc
     5221 caatgacgtc cacgggagcg cgctcgccga agcaaggtgc ggcgcagcgc gcggggcacg
     5281 cggcttcgtt tgggtgtatg tcggcaccgg agtcggctct ggcatcgtgc acggtggcga
     5341 gctgtttcac ggctgctccg gctacagcgg tgaaattggg cattgcccgg tgttggtcga
     5401 aggtcccgag tgcagctgcg gtcggcgcgg atgtctggag gcagtggcgt cggggtgggc
     5461 catcgctcgc gacgcaactc gcgcggtaca gagcggggaa cgcacggcct tggccgacgt
     5521 cgcgggcccg ctcgacgcac gcaccgtctc tgctctggcc gtcgaggggg acgaggtggc
     5581 ccgaaggatc ctgacccaag tgggcaccta cgtgggaatc gggatctcga ccttggtcaa
     5641 cttgttcgac cccgagctga tcgtgctggg tggcaaggtg atgttgggtg gtgattggat
     5701 agttcaggcc gcgcgcgagt ctctggcgaa gcacacggtg cggcgcgacg aggtgcgcat
     5761 cgtgacgtcc tcgttggggg accaggtggg tctgctcggt gcactcatga acgcaatgga
     5821 cttgtcggtg cgctcttatc gagtagtcgc taccggtgag cgcgtggcct agcgtaagcg
     5881 tcgggaattc tagcgcagcg ccgggagcgt ctccgtggcc tcacgcgcgc tccatcggcc
     5941 tacttctgga attcgtaaat cgaacccaga ccgaggattc cggtgagttc gtcgagggcc
     6001 cgcctcgatt cctccaaaag accggggtcg cgcaagtctt cgatccgtag gcggtcccgg
     6061 taatgccgac ccacccagtg gacgagctcc gcgtcgaggc ctgcctcgta aaagacgcgc
     6121 gcccggatcg ttgcccgttc gcgttcggtg agctgaacgc gcaggcgcaa gcaagcgggg
     6181 ccgccgccat tgttcatcga agcgttcaca tccacgaagc agagctgaac ccgcttgccc
     6241 acgcgggcct cgaggctttc gacgaagttg cgagccgcag tgttgccctg cgtctccagc
     6301 ggcgccacca gcaggtagcc atcagcgatt cggatcaact cgcagttgaa ggcgtacgaa
     6361 gccacggcgt ccacgacggg gaactcctgt tccgtggcca acacggcctt gaactccggc
     6421 ccgagtcgcg cgtcgagctc gctcaagaac cgagtaggat gcacgacggc ccgctcgtga
     6481 agcagcaaca acgagcccgc gcccacgcag agtacgtcgc tgtggaacgc ccctgcgtcg
     6541 atgccctccg gatcttgctg ccaaagtagc gcgctctcct cgaccctgag tagccgtgcc
     6601 aaggccgcgc ttgcgtcgcg cgtctgacgg gcgggaaagc gctgcgggag cgccgcgaca
     6661 cccgccgcgc gcccccaaaa cagcacatgt gcgtaaccct gcagtgtggt caagcgcgtg
     6721 tggttcgcgg cgccctcgtc cgccagggcg gcttggctcg gcagcggctc gtgcacgcag
     6781 aactgctgtt cgtcttggaa cagccgctgc agcatgcgcg aggttgcggg cgcttcgaag
     6841 ctacggtgca gcatcgagtt caggttcgcg ggtacgaagt ggacgcggcc gtcggatgtg
     6901 tcggagctag gcgcgaccgt ggccgcgttg gctgcccaca tcgaggaagc gctgctcgcc
     6961 gcgctgagca gcaatggatc gtgacggtac gcggcctcga gcacatcgcg atcggatccc
     7021 ctaaagccca aacggcgcag aaccggcaaa tagggccgct cctgaggagg gaagaccgcc
     7081 tttcggcccc cgagcgcgct tactcggcgg agcttctcca gaccctgcaa tgcggccgct
     7141 cgggggtgcc cgatatcacc ggcgtggctt tgactttgga ggttccccgg gctcaacccc
     7201 gcgtgatgat gggttgggcc aattagaccg tcgaacgcgt attcttccgc ggattctgtg
     7261 tggctaggac tcggctcgcg cgttctcact gcgaaaccct gtcgatgtct agcatgagct
     7321 gttcggtgag ctcgctccct gaacccgccc catcatcgtg attcgctgag ttacgattct
     7381 gcctacatcg gtccctggca gtggcccgcg cgactcgtgt tcagcccctt cgtgactcgg
     7441 ccatccgctc cgggtgactg agccagacgt cctcgggctt ggcactccgg tgccgccact
     7501 cgtggcacgg ccaatgagga gaaatgtcct catgagcgtc gcggtcccca cgaatcccga
     7561 tagtggtccg agaccccgtc ccgtagtact gtacgagtca tggtccggcg gcttgcagac
     7621 acttctccac aatctagggc cggcgaatgg tttccggaag tggggttgag cgggtgtcgg
     7681 ggcgttgctc gttgccgtgt cgctagtcga cgttcggtga gcggggggat gcagtgagca
     7741 agaccttcgt caaaggcgtg cgcgattggc tgttactcgc cgggttgggg tgctcgctcg
     7801 gcttgccagt aacttggtgc tcttcagcgc atgccgaaga tcagaaggag ctggcgcgag
     7861 cgcgtgtgca attccaacaa gcaatcgagc tcgagcaagc cggcaactac gcgagcgcgg
     7921 tgaagttgtt ccgggctgtc gggcaagttc gcatgactcc gcaggtgcgc tttcacatcg
     7981 cgctgtgtga agaaaagctc gggcgtttgg tcgcggcctt gggcggcctc gagttggctc
     8041 tgtccgaagc tgacagcgta ggtcccgagt ttcgctcgga agtcgaggcg aacgttagcc
     8101 gcctgcgtga gcgcatcccg aagctcgtga tccagcgtgg caccggcgct gaagccgcaa
     8161 ccatcgaatt ggactccgtg gtgttggggc aaggctcggt tggcgtcgat gtgccgctcg
     8221 atcccggtcc gcatccagtg acggcgcgcg cgcccggtta tcagccgttc gaatcgacgg
     8281 tgacgctcgt cgaaggcgaa aagaaggtac tcgagatcgt gctcgaaccg agcccagtcg
     8341 tgaccgaacc ccccgtcgct tcgggtccgg ctccggttcg tgcgccggaa aggaaaccca
     8401 acatcgttcc gttcgtcgtc ggaggtgtgg gcgctgcgac gctcgtcgct tccggagtct
     8461 tctttgcgct gcggcagtcg acgctctcta gtctgcagtc ggcttgtccg gtgcggctcg
     8521 agtgttcgca atccgaagag tcgaagtacg accgactcaa gatctatcac tacaccgcgc
     8581 tcacgacctt gggcgtaggt ctggcggcag tgggtaccgg tgcggcactc ttcgccatcg
     8641 acttgaacgc acgtaaggag tctgcggttg gactcaagat cgtgccgcgg ttctctccca
     8701 ctttcgcggg cgcgagtgcg cagctccgct tctactgagc gcccgcattt tcgcgcttgt
     8761 ccgcttcgcg ccgacccgct tgcgccccag accacccgct ggcttcgcga acgcgtagtg
     8821 ccgcgccgga acttatcgtc gccctctcac atgcgctgca cttagaaccc tggatcgata
     8881 cgccagtcgt tctttttgcg cggttgcgtc gtcggttgct tcggcggagt gcttccggcg
     8941 ggccgtggcg tgggtgggcg tgcgcccttg ctagcgctcg aatcaggcgt cgcggcgttg
     9001 tctcgctgag acccgccgtt cagcggggcg gcgtcctcgc ccgcggagct agatggcgct
     9061 gcgtccgggt tcgacctctc cgcaccagac ctatcggtct cggtctttcc cgggccggtg
     9121 gtgggctgag ccgctggcgc tacgctctga gtcggtaagg ccgtgctcgg cggcttgtct
     9181 ggcgtggata cccaacgaac cacggcgagc accaatgcgg cgctcgcgct caccaaggcg
     9241 gcgcccatca gtaacgtgcg gttgcgacgc cggcgcgcga aacccgaaag ctccgtggtg
     9301 gcgttggtgt caccacccgc gagggtgatc ccgtgattcg atatgctgcc gttgctcggg
     9361 tgagacggcg catccgtgag cttgggcaac agcgaagcgt tctggtcggc gcgcaccagc
     9421 gcatcactca acgtcgagcg acgctcttgc agcttgtcgc ccaccagctt acgcacgaac
     9481 tgggcgacgt catcgtccgt cagggctcgc tggtccgtcg gcagcgcacg gtccaggtcg
     9541 cgcaaaaact catttgcggt ctggtagcga tcggccgggt tcttcgcgag cgcacgcaac
     9601 accaccgcct cgagcgcgcg tggatagtcg ggcatcacgc gcgtaggagg taacgccgcc
     9661 tgaggcgagc agatgttgta catcgtggcc gcgtccgatt cgcggcggaa tggatgcttg
     9721 ccggtagtga gcgcgtacag cacgatgccg agcgcgaaga tatcggcgcg ccgatcgaaa
     9781 tcgtccccgt cgatttgctc gggcgccatg taccccacct tgcccttgag ctggccgggt
     9841 gaagtaccac tccccatgat tgcggtggct ttggccacgc caaagtccac caccttcgtg
     9901 accccgtcgt aggtcacgag gatgttttgc ggcgaaacgt cccggtgcac cagccccacg
     9961 agctgcccct gtggatctcg cagctcgtgc gccgcgtgta gccccgcgct ggcctgcatc
    10021 acgatgcgca cggcgatcga caaggggatc ccgcctgctc ccttgctcgc cttcatgagc
    10081 tggttcaacg gcacaccgtc gatccactcc atgaccagga acagcacgcc gttctgctcg
    10141 ccgagatcga ggatgtctac gacgtgcgga tggcggattt gcagcgcgag tgaggcttcg
    10201 tccaaaaaca tctgctcgaa ttgggcgtcc tcgctcaact tcggcagcat ggttttgact
    10261 gcgacgatct tctgaaaccc tcgtgtgccc ttcagtcgag cggcccaaac catggccatg
    10321 ccgcccgcgg caatgggcat gagcaattca tagcggccga gtacgtgacc gggagcaacg
    10381 atcccggaga ttaccgccgg ccctatgtcg gtcgccgagg tcactgaagt ggggttagtg
    10441 tgttgcaagt tgcagtgttg caaagcgaca atcgctgctc gaccatccgt aacgcaatga
    10501 acgatccggc cattccgagg ctacgccggc cgggacgcga agggggactc gatgagagcg
    10561 tggcgattgg atttgccaag tggagtgtgt cttacgaagg ccggtcttac gaaagcctgt
    10621 gtcttatgaa ggtatgtgtc ggagatgctg ggtggccgtg cttatctgtg gttgtgggga
    10681 atataatcgc gccgggtgta gttgtggggc cgcggtctgg caacgcgggc cgcgcgagcc
    10741 tgccagattg gctcggcagc aaaccgtaac gcagggctcc gaggagcgag tgcagcatcg
    10801 gagctcagtc gctcgacgca gtgcactgcg agaagcatcg ggaaaaggtg taaaaacacc
    10861 gtggccagag ggggtggggg cgaagttcga aaggtgttgc gggtgacggt gacccacgcg
    10921 acttctttcg tagcatttct ggtgaattct gccgttcttc gccacatcct ccgggtgaat
    10981 ctcgatgtcc gatgacgcgc cccagagcgg gtggatccgc cggcttttcg gctggtgtga
    11041 gcaacgctcc gcagagttga gccgcctacc agcgctgctt acccgggtaa ttcctgagct
    11101 ttacgtggcg cgtcagggga ctggcgggtc gtttctggac gtgctgtccg gagtagcggc
    11161 cacgcgcccc gacgcggtgg cgctgtggat gggggagaga cgaatcactt atgcgcagct
    11221 ggcgctcgag gtagacgtcc aagccgctcg tttcctcagg gctgggatga ggcccggcca
    11281 acgcgcggcg ctgtgtggaa gcaactcgct cgagtacgtc gtcgcactgc tcgccgcggc
    11341 acgcctcggc gtgcccgtgg cgctcccgga tttcgaactt tcgagcgagc tgctgggcga
    11401 agcgttgcga cgtgcgggcg cggagttcgt gctctgcgac gcggcgagtg aagcgcgggt
    11461 tcagtcggtc acttccgtgc ctcgatgcac catcggttcg atcgagaccg cgccgcaaag
    11521 tgcttcggag cgcgagccga gtgatcaacg tttgctgcgg gagatcgagc tagttggcgc
    11581 tcggccgggc tggaactcgg atttcgctta cgtgttcacg tccggcacga ccggcaagtc
    11641 caagccgtgt cgcgtcaccc atggccgggc gttgcttgcg tccagtgcgt tcggtcgttt
    11701 ggtcatgcgc ttacgccctg atgacgtgct gtactgcccg ttgcctctgt gtcactcgag
    11761 cgcgttgttg ctcgggcttt gtgcgtgttt gaggacgggt gcgacgctgg ttctccgaga
    11821 acgcttcagc gcacgccact ttctggctga cgtccgccgc acgcgagcca cggtgttgct
    11881 ctacgtcggt gagctcggtc gcgcgctggt ccagctgccg gagagccccc tcgaccgcga
    11941 acacgcgtta cggctcgcag tggggaacgg catgcacgag agcgtttggg aggggctgca
    12001 gcgacgcttc g
//
LOCUS       DASTKU010000964         9159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_411173, whole genome shotgun sequence.
ACCESSION   DASTKU010000964 DASTKU010000000
VERSION     DASTKU010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9159
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411173"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFQ61_34210"
     CDS             <1..432
                     /locus_tag="VFQ61_34210"
                     /inference="COORDINATES: protein motif:HMM:NF036850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959613.1"
                     /translation="FAAQFRALGAELLAYPDGDPSSAAQLAEVCRGTDVVLQATSAGM
                     HGTQGGDALAALVPFESLPAHALAYDLVYNPRQTPFLRAAAARGLEARHGLGMLVRQA
                     SLAMELWLGKRPSFETLYHAADTALGRPNAPSQRAGAKEAP"
     gene            429..2438
                     /locus_tag="VFQ61_34215"
     CDS             429..2438
                     /locus_tag="VFQ61_34215"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET9959614.1"
                     /translation="MKVRPGKDFRASHDGPWPALETNGELLPAEREWLHTNGAGAYSM
                     STIAFMHTRRHHGAFVATLPPPLGRHVIISHNETSLVPESDRRTYRLSTHQFPNLAPT
                     PGYRYLHSFAIDPLPRWVFRIGHQQLERTLCLARGRNALVASYTWRGRGSALLQVRPL
                     MPLRPVDKLMREHGGMMQVVSLKPAAVEMKPVPELPSVVFGHEGMFMGSPDWWRRFEY
                     LADRSDGIEFQEDIWTPGVFEMQLEPNRPVYLIAAIGALPETSPAELMAEARAAIQAN
                     DPGDERPSSIRVLSIAADQFCLDRLAKPAVIAGYPWLAPLVRDWVMALPGLHLTRGRF
                     QEFERALRTLLPALRGGLLPERLPEPGARRGKPLPDSSLWLFEVARELERARGIDDEC
                     LKKCLYPALVRVFARVRWRRRRYVWLSKEGLVVNGARNLALTWMDAHIGAELVTPRAG
                     AAIEHQALWVQGTRTLARLADHYGYPKLAAVAREHCETTIKAFRARFWCSETDYPYDC
                     VSEARDRTDAWADPSIRPNALIALAIEPALFEDWQAHSIVERVRAELLTPRGVRSLSP
                     SDRRYIGQFAGSPEERELAYHRGTAWTHLLSFYARAVLRLAGDDADVYYDLRALIEQA
                     LDDGALLGQVGQLADGDAPHRSRGCPAQATSVAALLQTLVVDLGI"
     gene            2876..3619
                     /locus_tag="VFQ61_34220"
     CDS             2876..3619
                     /locus_tag="VFQ61_34220"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9959615.1"
                     /translation="MNFSCEDPDDPIDYPVEEKVGEGLLQTWLVESLRPLVERWLAER
                     GEVALVGADQFIYFEKGQPGVRVSPDVYVLPGVPPDTCIDCWKTWLTGVVPSFALEVV
                     SKKARKDYVLTPQAHQQLGTAELVIFDPKPHLRRTGVRFQRFERTGGRLQLCERTDAD
                     RVYSSVLECYLKMVPWRGVPQVRLALPPDGTALYPTALEAERAARMRAEAERGATQAR
                     IAELEAALARARGDEPTRTDSRPSESDQK"
     gene            complement(3610..4275)
                     /locus_tag="VFQ61_34225"
     CDS             complement(3610..4275)
                     /locus_tag="VFQ61_34225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959616.1"
                     /translation="MKKLLANGVLVVGLLASKGAWAQARFAEPHSFAISAERLFGYSH
                     SSFTDEDAPGEPTTTNNRFSFLMSASDPSPWIAFDYFPIRGLSLGGAVAFASESGKVK
                     SPGVTRDQPDQFSFLIMPRVGYAIMFNQHVGIWPRGGIGYTSTKISDDTTERARRSWI
                     ATIEVPFVFSPVPHWAFTVGPTFDFALSGTDDVKTIANNVTQSRDFSANRFGLYFGML
                     GYF"
     gene            complement(4587..5756)
                     /locus_tag="VFQ61_34230"
     CDS             complement(4587..5756)
                     /locus_tag="VFQ61_34230"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET9959617.1"
                     /translation="MQAKQRNIPLAMPLTGEAEADAARRPILSGWLTQGPEVAAFEQE
                     FASYVGAPHAVALANCTVALHLALLAVGVKPGDDVITVSHSYIATANSIRYCGAEPVF
                     VDVDYATSNMDPVRIEPALTKNTKAILCVHQMGMPCDVEAIVEIGRKHGLPVIEDAAC
                     ASGSAWIKKDGQVEPIGKPHGDIAAFSFHPRKVITTGDGGMLTTKHAEFDKQFRLLRQ
                     HGMSVSDRARHGAREVVFEDHSVLGYNYRMTDVQAAVGREQLKRLPGIVDARRRLAAR
                     YAEHLRELTDITLPVEPSYARSNWQSYAIALSPALPQKKLMQALLDRGVASRRGIMNA
                     HREEPYRNNLSGKNLPNSEAAQDRKIILPLYAQLTDSDQDYVIASLREAIREVRG"
     gene            complement(5760..6740)
                     /locus_tag="VFQ61_34235"
     CDS             complement(5760..6740)
                     /locus_tag="VFQ61_34235"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9959618.1"
                     /translation="MTSSPNRVLVTGGAGLIGSHIVDLLMTERERGTVSDIIVLDNFV
                     RGRRENLAKAMARGEVTIVDGDIRDRALVAKLMAGVDQVFHQAAIRITQCAEEPRLAL
                     EVLVDGTFNIVEAAVAAKVKKVVAASSASVLGLAEDFPTTERHHPYNNRTLYGAAKAF
                     NEGLLRSFNEMQGLKYVALRYFNVYGTRMDIYGAYTEVLIRWMDRILEGKPPLIFGDG
                     KQTMDFVYVEDIARANLLAMQSDASDEVFNVASGTETSLNDLAFALLKVMGRTDLKPE
                     YGPERKVNPVPRRLASTKLAKDRLGFEAKIGLEEGLQKLVAWWRSEKKLG"
     gene            complement(6801..7901)
                     /locus_tag="VFQ61_34240"
     CDS             complement(6801..7901)
                     /locus_tag="VFQ61_34240"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET9959619.1"
                     /translation="MIPFVDLKTQYESIKPEIDAAVLNVFATTQFVLGSAVASFEKAF
                     ASYCSAEQAIACSTGTSALHLALLAAGVGRGDEVITTPFTFLATVSAIDYTGAKPVFV
                     DIANDSVTIDPSKIEQAITPKTKAILPVHLHGCPADMDPILEIAKKHNLIVIEDAAQA
                     HGAEYKGRRCGSMGALGCFSFYPGKNLGAYGEGGAITTSNAEYAKTLRKLRDWGSDRK
                     YYHDLKGFNYRMEGVQGAVLGVKMKYIEGWTESRRRLAARYNELLAGTPYGLPPSVPN
                     SRHVYHVYAVRHARRDALQSYLQEKGVATGIHYPVPVHLQRCFAELGHKPGDFPNSER
                     AANEVLSLPMFPELTDAQQDVVIAALKEWPAK"
     gene            complement(7906..8925)
                     /locus_tag="VFQ61_34245"
     CDS             complement(7906..8925)
                     /locus_tag="VFQ61_34245"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9959620.1"
                     /translation="MIGIGVIGYGYWGPNLVRNFSQAEGSRVVAVCDPRTDRRKHAVS
                     TYPAITAVETVPELLANPAVDAVAIATPVSTHFDLAMQALQAGKHVMVEKPMTSNSDQ
                     ARRLIDEADKRNRVLLVDHTFVYTSVVRKIKELVDSQQLGKLYYYDSVRVNLGLFQHD
                     VNVLWDLAVHDLSIMDFVLGRPPQMVAATGVASVPGHPESLAYLTCFFDNDLIAHFHV
                     NWLAPVKIRSTMIGGSDKMILWDDMELSEKLRVYDKGITLNSGPEGVYQLKVGYRAGD
                     MWAPHVPNVEALQDEAKHFIRCINDNRTPLTDGRAGLRVVKILEAASQSMAEHGKPVR
                     LTIEE"
BASE COUNT         1526 a         2905 c         2973 g         1755 t
ORIGIN      
        1 tttgccgcgc aatttcgcgc gctcggcgcg gagctcttgg cctatccaga cggcgatcct
       61 agctcggccg cgcagcttgc cgaggtatgt cgcggcacgg acgtggtgct tcaggccacg
      121 agcgccggca tgcacgggac ccagggtgga gatgctttgg cggccctagt tccgttcgag
      181 agcttgcccg cccacgcgct cgcttacgac ctggtttaca acccgcgcca gacgccgttc
      241 ttgcgtgcgg ccgcggcgcg cgggctcgaa gcgcggcacg gtctcggcat gttggtgcgg
      301 caagcctcac tcgcgatgga actgtggctg ggcaagcgcc caagcttcga gacgctctat
      361 cacgccgctg acacggcgct gggccggcca aacgcgcctt ctcagcgcgc gggagccaag
      421 gaggcgccgt gaaggtccgg ccgggtaaag actttcgcgc ctctcacgat gggccctggc
      481 cggcgctcga gaccaacggc gagttgctgc cggcggagcg cgagtggttg catacgaacg
      541 gcgcgggtgc gtactcgatg agcaccatcg cgttcatgca cacgcgtcgt caccatggcg
      601 cgttcgtggc cactttgccg cctccgctcg gtcgtcacgt catcatcagc cacaacgaga
      661 ctagcctggt gcccgagtcc gatcgcagga cttatcgtct ctccactcac cagttcccaa
      721 acctcgcgcc cacccccggc taccgctacc tgcatagctt cgcgatcgat ccgctcccgc
      781 gctgggtgtt tcgcatcggt caccaacaac tcgagcgcac gctctgcctg gcgcgcgggc
      841 gcaacgcgct cgtcgcctcg tacacctggc gtggccgcgg ctccgcgctg ttgcaagtgc
      901 ggccgttgat gccgcttcgc cccgtcgaca aactcatgcg tgagcacggg ggcatgatgc
      961 aggtggtgtc gctcaaacct gccgcggtcg agatgaagcc ggtgcccgag ctgccctcgg
     1021 tggtcttcgg ccacgaaggc atgttcatgg gttcgccgga ttggtggcgc cgcttcgagt
     1081 acctcgcgga ccgcagcgac ggcattgaat tccaagaaga tatctggacc cctggcgtgt
     1141 tcgagatgca gctcgaaccg aatcgccccg tctacttgat cgcggcgatc ggcgcgctac
     1201 cggagaccag ccccgccgaa ttgatggcgg aggcgcgggc cgcgattcag gccaacgatc
     1261 cgggggacga gcgtccctcc tccattcgcg tactgagcat tgcggcggac caattttgct
     1321 tggaccgatt ggcgaagccg gcggtgatcg cgggctaccc gtggctcgcg ccgctggtgc
     1381 gcgattgggt catggccttg cccggtctcc atctcacgcg cggccgcttc caagagttcg
     1441 agcgcgcctt gcgcacccta ctgcccgctt tacggggcgg cctgctgccg gagcgcctgc
     1501 ccgagccggg ggcccgccgc ggcaagccgc tgcccgacag ctctctgtgg ttgttcgagg
     1561 tcgctcgtga actcgagcgc gctcgcggca tcgacgacga atgcctcaag aagtgcctat
     1621 accccgcgct cgtccgcgtc ttcgcgcgcg tgcgctggcg tcggcgccgc tacgtgtggc
     1681 tctccaaaga gggcctggtc gtcaacggcg cgcgtaacct ggccttgact tggatggatg
     1741 cgcatatcgg ggccgagctc gtcacgccgc gcgccggcgc cgccatcgag caccaggcgc
     1801 tgtgggttca gggcacgcga accctcgcgc gcttggcgga ccactacggt taccccaagc
     1861 tcgccgccgt cgcgcgcgag cactgcgaga ccacgatcaa agcgttccgc gcgcgcttct
     1921 ggtgcagtga gaccgactac ccatacgatt gcgtgagtga agcccgcgac cgcaccgacg
     1981 cctgggccga cccgagcatc cgccccaacg cactgatcgc gctcgcgatc gagccggcgc
     2041 tgttcgaaga ctggcaagcg cactccatcg tcgagcgagt gcgcgccgaa ctgctcactc
     2101 cccgcggcgt gcgcagcctc tcgcccagcg atcgccgcta catcggccag tttgcgggca
     2161 gccccgagga gcgcgagctc gcctatcacc gcggcacggc ctggacgcac ctgctcagtt
     2221 tttacgcacg tgccgtgctg cgcttggccg gtgacgacgc cgacgtttac tacgacttgc
     2281 gcgcgctgat cgaacaagcg ctcgacgacg gagcgttgct cggccaagtc gggcaactcg
     2341 cggacggcga tgccccgcat cgttcccgcg gttgccctgc gcaggccacc agcgtcgcgg
     2401 cgctcttgca aacgctcgtc gtagatctcg ggatctgagc gccactcgag cgcccgtgga
     2461 ttcgagggtt gcctctgctt ggattggcac gcgcaacaac gggcgcgctg ggccattgag
     2521 tacgtcactc gcgcagcccc tcgccggcgc tccacgtctc aacaccgcgc tcgcccggcc
     2581 ctgttgacgc ccggccctgt tgacgctcgc tgtgcgagga tgaagcgcgg gattcaaact
     2641 cgttatcccg attccgtctc tccgcgccaa ggatgactct gcgtatcccg ctgcggataa
     2701 cgggacatgc tccgtgtccg tatctcaaaa cacgagaatt aggctggttt tgtcgctggt
     2761 tcaggctacg gtgtgcctcg gcagtcgcca gttggggcga ttgctctttc tcgcgagttc
     2821 tcgaatgctg aacttgaagg cacgcgttcg ttcgcgcatg gtagagttca ttcccatgaa
     2881 cttttcctgc gaagatccgg acgatccgat cgattatccg gtggaggaaa aggtggggga
     2941 agggctatta cagacgtggc tcgtggaatc actgcgtccg ctggtagagc gttggctcgc
     3001 ggagcgcggt gaagtggcgt tggtcggcgc cgatcaattc atctacttcg agaaggggca
     3061 gcccggcgta cgcgtttcgc cggatgttta cgtgctgccc ggagttccgc cggacacctg
     3121 catcgattgc tggaagacgt ggctaactgg agtcgtgccc tcctttgcgc tggaggtcgt
     3181 gtccaagaag gcgcgcaaag actacgtact cactccgcaa gctcatcaac agctcggcac
     3241 tgccgagctc gtcatcttcg atcccaagcc gcacttgcgg cgcaccggcg tgcggtttca
     3301 acgcttcgag cgcaccggag gtcggctgca gctctgcgag cgaacggatg ccgatcgcgt
     3361 ctactcgtcg gtgctcgaat gctacctgaa gatggtgccg tggcgcggtg tgccccaagt
     3421 tcggctcgcg ctgccacccg acgggacggc gctttacccc accgcactgg aggcggagcg
     3481 tgccgctcgg atgcgggcgg aggccgaaag aggggcaacg caggcccgca tcgcagagct
     3541 cgaggcggcg ctcgcgcggg ctcgaggaga cgagcccacg cggacagact cacgcccaag
     3601 cgagagcgat cagaagtagc cgagcattcc gaagtacagg ccgaatcgat ttgcgctgaa
     3661 gtctctgctc tgcgtcacgt tgtttgcgat cgtcttgacg tcatccgtgc cgctcagcgc
     3721 aaagtcgaag gtaggcccaa cggtaaaggc ccaatgaggc accggtgaga aaacgaaagg
     3781 aacctctatc gtcgcgatcc aggaccgcct cgcccgctca gtcgtgtcat cagagatctt
     3841 ggtggaggtg tagccgatgc ctccgcgtgg ccaaatcccc acgtgttggt tgaacatgat
     3901 tgcgtagccc acgcgcggca tgatgagaaa gctaaactgg tcgggttggt ctcgggtcac
     3961 gccgggggac ttgaccttcc cggattcgct ggcaaacgca accgccccgc ccaggctcaa
     4021 gccacgaatc ggaaagtaat cgaaggcgat ccacggcgac ggatcgctcg cgctcatgag
     4081 gaaggagaac cgattgttgg tcgttgtcgg ttcgccgggg gcatcctcgt cggtgaagct
     4141 ggaatgcgag tatccaaaca gccgttcggc gctgatggcg aagctgtggg gttcggcaaa
     4201 gcgagcctga gcccaagctc ccttgcttgc gagcaacccc acgaccagga ctccgttggc
     4261 tagcagcttc ttcaagttac ctctcccagt gaatcgctcg aaggtagccg aagataggca
     4321 ttcggtgaaa gctcttcacc gaggtggggc tacgcgccct gtcccgaacg gccgttattg
     4381 cgggccgttt gcgcggtgat gcgagaactt aggagggata acggactgcg ctggtggctg
     4441 taggcgacgg ctctcgcgcc ccgttacttc gagcggccat tcaaatgcgg ctccggactc
     4501 acgatccgag ggcgcaaatg cagaaatccc cccgcgcgtt ccgcgaggga gtgcgcgagg
     4561 ggaaacctgg tttcgctgat tactggctag ccgcgaactt cgcgtatggc ttcgcgcaaa
     4621 gacgcgatca catagtcttg atcgctgtca gtcagctgag cgtagagcgg gaggatgatc
     4681 ttgcgatctt gagcggcttc ggagttcggc aggttcttgc cgctgaggtt gttgcgatac
     4741 ggctcctcgc gatgggcgtt catgatgccg cggcggctcg ccactccacg atcgagcagc
     4801 gcttgcatga gtttcttctg cgggagcgcc gggctgagag caatcgcgta gctttgccag
     4861 ttggaacgcg cgtagctcgg ttctacgggc aacgtgatgt cggtgagctc gcgcaaatgc
     4921 tcggcgtagc gtgccgccaa tcggcgacgc gcgtcgacga tgcccggcag ccgcttgagc
     4981 tgctcgcgtc ccaccgcggc ctgcacgtcg gtcatgcggt agttgtagcc gagcaccgag
     5041 tggtcctcga acaccacctc gcgtgcgccg tggcgggcgc gatcgctgac gctcatgccg
     5101 tgttggcgga gcagtcgaaa ctgcttgtcg aactcggcgt gtttggtggt gagcatgccg
     5161 ccgtccccgg tggtgatcac cttgcgcggg tgaaaggaga aagcggcaat gtcgccgtgg
     5221 ggcttgccga tcggctccac ctgaccgtcc ttcttgatcc acgcgctgcc gctggcgcac
     5281 gcggcgtctt cgatcacggg caagccgtgc ttgcgcccga tctcgacgat cgcttccaca
     5341 tcacaaggca tgcccatctg gtgcacgcac aagatggcct tggtgttctt ggtgagcgcc
     5401 ggctcgatgc gcaccgggtc catgttggag gtggcgtagt ccacgtccac gaacaccggc
     5461 tcggcgccgc aataacggat gctgttcgcg gtcgcgatgt acgagtggct gacggtgatg
     5521 acgtcgtcac ccggcttcac gcccacggcg agcaacgcca agtgcaacgc gaccgtgcag
     5581 ttcgcgagcg ccaccgcgtg tggcgcgccg acgtagctcg cgaattcctg ctcgaaagcc
     5641 gccacttcgg ggccttgggt gagccagccg gagaggatcg ggcgccgagc cgcgtccgct
     5701 tcggcctctc cagtgagagg catggcgagc gggatgttgc gttgcttggc ttgcatggtt
     5761 cagcccagct tcttttccga tcgccaccaa gcgaccaact tttgcaggcc ctcttcgagc
     5821 ccaatcttcg cctcgaagcc gaggcggtcc ttggccagtt tcgtggaggc gaggcggcgc
     5881 ggcaccgggt tgaccttgcg ttcggggccg tactccggct tgagatcggt gcgacccatc
     5941 accttgagca gcgcgaacgc gagatcgttc aggctggtct cggtgccgct cgcgacgttg
     6001 aacacctcgt ccgaggcgtc cgactgcatg gcgagcaggt tcgcgcgcgc gatgtcctcg
     6061 acgtagacga agtccatcgt ctgcttgccg tcaccgaaaa tgagcggcgg cttgccttcg
     6121 aggatgcggt ccatccagcg gatcaacacc tcggtgtagg cgccgtagat gtccatgcgc
     6181 gtgccgtaca cgttgaagta gcgaagcgcg acgtacttca gcccctgcat ctcgttgaag
     6241 ctgcggagca ggccttcgtt gaaggccttg gctgcgccgt acaacgtacg gttgttgtac
     6301 gggtggtgcc gctcggtggt ggggaagtct tcggccaggc ccaagaccga agcggacgag
     6361 gcggcgacga cctttttgac cttggccgcc accgccgctt ccacgatatt gaacgtgccg
     6421 tcgacgagca cctcgagggc caggcggggt tcctccgcgc attgcgtgat gcggatcgct
     6481 gcttgatgga agacctggtc gacgccggcc atcaatttgg cgaccaacgc gcgatctcga
     6541 atgtccccgt cgacgatggt gacctctccg cgggccatgg ctttggccaa attctcgcgc
     6601 cggccgcgca cgaagttgtc caaaacgatg atgtcactga cggtgccgcg ttcacgctcc
     6661 gtcatcagca ggtcgacgat gtgcgaaccg attagaccgg caccaccggt cacgagcacc
     6721 cgattgggcg aagaggtcat actcactcct tgaattcttt gcttctgaga ttgcttcgga
     6781 gaacggaaac cttcagcggt ttacttcgcg ggccactctt tgagcgcggc gatcacgacg
     6841 tcttgctgcg cgtccgtgag ctccgggaac atcggtagcg agagcacctc gttggcggcg
     6901 cgctcggagt tcgggaaatc tcccggcttg tgcccgagct cggcgaagca gcgctgcaga
     6961 tggaccggca ccgggtagtg aatgcccgtc gcgacgccct tctcttgcag gtacgactgc
     7021 agcgcgtctc ggcgcgcgtg gcgtacggcg tacacgtggt aaacgtggcg cgagttcggc
     7081 acgctggggg gtaagccgta gggcgtgccc gcgagcagct cgttgtatcg agcggcgagg
     7141 cgacggcgcg actccgtcca gccctcgatg tacttcatct tcacgccgag cacggcgcct
     7201 tgcacgcctt ccatgcggta gttgaagccc ttgaggtcgt ggtagtactt gcgatcggaa
     7261 ccccagtccc gcagcttgcg cagcgtcttc gcgtactccg cgttcgaggt cgtgatcgcg
     7321 ccgccctcgc cgtaagcgcc gaggttcttg cccgggtaga agctgaagca gccgagcgca
     7381 cccatgctgc cgcaacgccg gcctttgtac tctgcgccgt gggcctgcgc ggcgtcttcg
     7441 atcactatga ggttgtgctt cttggcgatc tccaggatcg gatccatgtc cgcggggcaa
     7501 ccgtgcaaat gcaccggcaa gatcgccttg gtcttcggcg tgatggcttg ctcgatcttg
     7561 ctcggatcga tggtgaccga gtcgttcgcg atatcgacga acacgggctt tgcgcccgtg
     7621 tagtcgatgg cggagacggt cgcgaggaag gtgaatgggg tggtgatgac ctcatcaccg
     7681 cggcctacac ccgcggccaa caaggccaga tgcagcgcgc tggtgccagt gctgcaggcg
     7741 atcgcctgct ccgcagagca gtaactggcg aacgccttct cgaagcttgc gaccgcgctg
     7801 cccagcacga actgggtcgt cgcgaagacg tttagaacgg ccgcgtcgat ctcgggcttg
     7861 atcgactcgt attgggtttt caggtcgacg aaggggatca cgctttcact cctcgatagt
     7921 cagacgaacg ggtttgccat gctccgccat cgattgtgag gcggcttcca aaatcttgac
     7981 cacgcgcagg cccgcgcgac cgtcggtcag cggggtgcga ttgtcgttga tgcaacggat
     8041 gaagtgcttg gcttcgtcct ggagcgcttc cacgttgggc acgtgcgggg cccacatgtc
     8101 gccagcgcgg tagcccacct tcaactggta aacgccctcg gggccgctgt tcagcgtgat
     8161 gcccttgtcg taaacgcgca gcttctcgct gagctccatg tcgtcccaca agatcatctt
     8221 gtcggagccg ccgatcatgg tgctgcggat cttcacgggc gcgagccagt tgacgtggaa
     8281 gtgagcgatc aagtcgttgt cgaagaagca ggtcaggtac gccaagctct ccgggtgccc
     8341 cggcacgctc gccacgccgg tggccgcgac catctgaggg gggcggccga gtacgaagtc
     8401 catgatcgac agatcgtgca ccgccaagtc ccagagcacg ttcacgtcgt gttggaacag
     8461 gcccaggttc acgcgcacgg agtcgtaata gtagagcttg ccgagctgct gcgaatccac
     8521 cagctctttg atcttgcgga ccacgctcgt gtacacgaac gtgtgatcga ccaagagcac
     8581 gcggttgcgc ttgtcggcct cgtcaatcag gcggcgcgcc tgatccgagt tcgaggtcat
     8641 gggcttctcg accatgacgt gcttgccggc ctgcagggcc tgcatcgcca ggtcgaagtg
     8701 cgtcgagacc ggggtcgcga tcgccaccgc gtcgaccgcg ggattggcga gcagctcggg
     8761 tacggtctct acggccgtga tggccgggta agtcgataca gcgtgcttcc tacgatccgt
     8821 acggggatca cagaccgcga ccacgcgcga gccttcggct tgcgagaagt tgcggacgag
     8881 attgggcccc cagtagccgt agccgatgac accaattcca atcacgatgc gtgcctttct
     8941 tgaattgtct cgaccgagtc gttcgaagtg agtcgttcga agtgctgagc cgctcgggac
     9001 taacgctgcc gtgagctccg agttcgctcg agtgccggtt atcgcgcggc cctccgagtc
     9061 aggcggcgct caatcggacg gcctcctcaa tggaggggcc ggcggcgcta ttcccgagac
     9121 cgcgggcttt cgcttcgaag cgcgcggctt tgcgcagac
//
LOCUS       DASTKU010000965        18291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_858659, whole genome shotgun sequence.
ACCESSION   DASTKU010000965 DASTKU010000000
VERSION     DASTKU010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 18291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18291
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858659"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            918..1823
                     /locus_tag="VFQ61_34250"
     CDS             918..1823
                     /locus_tag="VFQ61_34250"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9959621.1"
                     /translation="MGRLDQVRSVNAEGAKAAQTVSFQRRSDLAGVEIRSVENAQEEA
                     FFYSPDFEFFVPITWRGEVWHRRRRIQLEPGSILIAQPGEAFLCRRAQEAGNHISLSV
                     EAEVMARFAELCGVAMADFAFSADGLLSDALSRCLRALTDCFKSTAATEPVEVHLMEW
                     LTLVFQQLRVGSAAPPAGASLERPPAERVRECLHEDLSATLDMSRVSRETGLSRFQAL
                     RAFKSWYGLPPCAYQLRVRLGLAQRLLRHGARPAAVAAEYGFVDQSHLTRHFRRWLGI
                     TPAEYARVGIEPSALRTNDLSLLAG"
     gene            1912..2289
                     /locus_tag="VFQ61_34255"
     CDS             1912..2289
                     /locus_tag="VFQ61_34255"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9959622.1"
                     /translation="MPIQALNHFTVLTRDLDASLHFYVRVLGLTIGARPELPSSGAWL
                     YLGEQAVLHVIVDPRAPERNGVIDHVAFTGVNLPEVTARLRSHGIQFELVRQPGSGTW
                     QLFLFEPSGGLLEINFDASESEG"
     gene            complement(2308..6348)
                     /gene="hrpA"
                     /locus_tag="VFQ61_34260"
     CDS             complement(2308..6348)
                     /gene="hrpA"
                     /locus_tag="VFQ61_34260"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HET9959623.1"
                     /translation="MCPEPVIPAERGPAQPLTDPSGSEQHGSEQHGSEPDPARMPPSA
                     PAGPALSNGLRIEFPPELPISARVADIGRAVAESQVVIVAGATGSGKTTQLPKLMLAL
                     GRGSRRRIGVTQPRRIAATSIAQRVASELGTNVGQEIGYQIRFEDRSHSRTRVKFMTD
                     GILLAETQTDRLLRQYDTLIIDEAHERSLNIDFLLGWLKRILSERPDLKVVVSSATLD
                     TQRFAEFFGQAKVIEVEGRTYPVEVLYEAPDPELDLSEAVGNAVVNLTSLDNRGDVLV
                     FLPGEREIHEAERELQGRKLRHTAILPLYGRLSSAEQRRVFTSGPERRIILATNVAET
                     SLTLPGIVYVVDAGYARLSRYDPRTGTTRLALEAISQASADQRKGRAGRVREGLCLRL
                     YDEESFRARPAFTDPEVLRVGLASVILRMKALGLGAVEEFPFLDPPAPRAITEGYRVL
                     QELGALNAERELTDAGRRLARLPVDPRIGRMILAGAEYGCLPEVLVVAAALSVQDPRE
                     RPRAAEQKADQQHQRFRDERSDFVGWLKLWDFLREVERSAGKAGLRRACKDGFLNFIR
                     VREWQEIHRQLLELAEELRLPLRPGGRDSPHRDPRPRDNSARDNSARDRQHRSPQHGG
                     RPRESQNGNLRGRDNALHSSPKKAEVQAGAGGDSPANAGDTSDALHMALLSGLLSKVG
                     YYNSTERVYVGAKQTRFVLHPSSALAKRPPAWVMAFELVHTSQLFARNAARIDPNWFE
                     RVGSHLLKRSYEKPHWSEKSGRTVIEERSTLFGLPVPTRRQVDYATISSSDARRWFID
                     HALVRGEYKSKGAFEGHNRALLAEVARLRDKARQSDMLADDEALFAFFDRLLPASVVN
                     GKTFESFREEIERTQPKALHLQLSDVLASEQGLTEAHYPDQVEVSGERIALHYLFDPS
                     AETDGLTLDVPLLLLPSFDPRELDWTIPGWHLQRITALIEALPKALRKELGNVNELAA
                     RAAERLTPFRGPFLAQLAQTVSELACVNVPESAFRPETLPPFLRPTLRVVDNSGHALG
                     QSRELAMLFEQLLPKAREELRALAPVSNLERTKLTAWNFGELPAIVERRSGGLVIKSY
                     PALMDRVTHVDLLLLDSSSEAERATRAGVRRLLRLVTRRSLSSLNAKVPRGFAPTSGR
                     TMLNSPDFEEQFWNKVFDQAFGLQTLEHLPTNQVQFEQLLGEGSPRIAQAFNTLNHAV
                     LAIAGELEATQRVLARAAEQPMGRAVASSVRAQLEQLLPADALSNLEITRLAQIPRYL
                     KAAQTRVQRALADPRKDAEKLAPIESLVREFQALRAPADARASEARARVRWQLEELRV
                     AIFAPELKTGPAVTVASVGQAIQALK"
     gene            complement(6386..9157)
                     /locus_tag="VFQ61_34265"
     CDS             complement(6386..9157)
                     /locus_tag="VFQ61_34265"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9959624.1"
                     /translation="MSQNGQAWHLSPAQQPRDRVPDGPSLLGRRLGSYLIERALSSSA
                     SGSVFAARHVHLNREVAVRFLAPELQGDAEFPKRFVSEARSLAKLQHHNLLEVLDIGE
                     VEGRSYYVSEFGRGQSLRAALRARSRLTPAEVVRYLGPVCEALGAAHEAGLVHRDLRP
                     EHVFVHEAGGLTVKLGGFGSTPLLPSGAGRTGAQVLSSPRYAAPEQANLELHQIPPVS
                     DLYALGAIAFELLTGKPVFEHASDLMLLVMHLRDPVPKLREHVPEVPEELAEVIERCL
                     SKHTEARPPSAAALARELGRLQDRAAPAEREREAREIVDLTQSMKIALRAGPPPSSSV
                     GRVPVPPAPDAAIHELVEPQSGRYTSHETDEVSVDSRAFPESEGTPPSQPEPLDTPLG
                     GTAQSTVKKLRLRMQRKGDFPAFSQNVGEVSKRADAASIYSASQLGASILNDFALTAK
                     LLRVVNSTYANRFGGKIYRVQHAIVILGFDRIRSLALGISLFKSAGQDPHAARIAESA
                     VGSLVSGEIARQLARDVTLNDEEAMVCAMFRNLGRHLVLIYLPEMYDQILQVMAKERI
                     NLRHAAEKVLGLSFGRLGVEIAESWHLPSRMVTAIASAPTAGSNPVRAEDRLNALSEF
                     ASEVCELIANEPAPSRKAKLGKLLLQYKDFLKLTPDELLERLASVEQAFLDRYSNVLG
                     PELKKSRLLAGVSQLVPPVQSPEGALAPNGRGNAPPGVAVLGGEEPEAAPKHVVPVQI
                     AKPQNAGRAPTPEQMTRWEARFEQLRTQVRTGLSGDRLLSAALKLASEILGLERLLVL
                     ATTGNKAELSVRFGFRDDLEAVAKELRFPIYPTRNVSVFYSAYHSGRDVVITDAFSPK
                     ATASIPVSYYEVLGSPAFALYGCFGKGLQPCLLLAEASSGAELPTPERVQLLGELRTW
                     IAQGAASRS"
     gene            complement(9363..10154)
                     /locus_tag="VFQ61_34270"
     CDS             complement(9363..10154)
                     /locus_tag="VFQ61_34270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959625.1"
                     /translation="MRKIAGSLVVSLIAFSSLLAACGGSDDPASGAGGNAGASTGGKS
                     STGGHSNTEAGSGSENPETGGTSGEETGGKTSTGGKTGTGGKTSTGGAASSGGKTGSG
                     GVASSGGRTGTGGGVSTPLSDSTPLSDIDTDSELQAFCNKAADRFKDVDVSKLNQGIC
                     AFTGLLMEAAGQGDCESAQAECLAEEEPEDPAGDCSGELPQCDNVTVKEFLDCNEAYT
                     KQGLAVYSSLTCDSDLSEFEAEPEIPEVCKGPFERCPELAPQSGS"
     gene            complement(10522..11049)
                     /locus_tag="VFQ61_34275"
     CDS             complement(10522..11049)
                     /locus_tag="VFQ61_34275"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET9959626.1"
                     /translation="MKHAWLARLVLGAALFAAGSLRPRTAAADILGSVSAGALGGYGF
                     DDALKVGIGARAGYTFPFKLYAGGTFVYHLGKTESLAGIDVKANVYYYGVEAGYDIDT
                     VPLLIVRPYLGLGRVTGVAKVGDESSTDSKFGVWPGAAAIIPLGGLFVGADARYLIVS
                     EYDAFSVFGSVGLKF"
     gene            complement(11147..13216)
                     /locus_tag="VFQ61_34280"
     CDS             complement(11147..13216)
                     /locus_tag="VFQ61_34280"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9959627.1"
                     /translation="MSAKTSVTIRLKLGLLAGLPVLGALVLAAILVRDARLQVQKAEG
                     LGTIEHLAQLSNAIGQLVNALQTERAQLCWLTGQKRHARAPRDSELPDFAQPAALPPS
                     SPTSAPTQGPENLEREYSATDKALKKLDAFLQGSSSVGMPERLGRDLREARKSVEALR
                     RGRLGATDETDFHVLFERYGSANRALIQSTAALTDLTDNGELLRPINSLVAVLELTER
                     ASQEQGLLAHVFALGRFPPGSYRSLVTLLSERKVYEDVFRTSASAEQLASYERALKTH
                     EQLRASELRKVALESTTDELTVDPADWLRSQDGTLKTLNDISRQLNTQVKSVAVEKIA
                     ESRRAEIGSISLVGIVLLVSVTLAGFIARGVTRSVRELHEASRKVGQGDLNVRVQVSS
                     EDELGAFGHSFNAMINEIARARAELGERMRMARELEIAASIQRALLPPSPEHPDFEFA
                     GRMAPADEVGGDFYDVLHSEDHALWITIGDVSGHGVDAGLVMLMTQAAFASQFRADAK
                     TSPGHALRNVNSLLCENIGNRLKDSKYVTAQLLAYEGKGRFLCAGAHQFPIVFRAASA
                     TCETFEVPGPWLGIQNELDEIPLTAIELNDSDILCLYSDGLTEARNLEGELFDTPRLL
                     AAIRDASSRYQSLEEIADAVISKVTEYAARREDDWTLLLVRHTPQHVRRSASKSRASS
                     RLNGYAS"
     gene            13464..13970
                     /locus_tag="VFQ61_34285"
     CDS             13464..13970
                     /locus_tag="VFQ61_34285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959628.1"
                     /translation="MESDHLPASIRVGRFEPSVYVRFRPAWTYIEGIREFGRFFCGAT
                     FNEPEVAERARVIIQETLENAVKYSQRTAHSELELTISSDGKRLEISVCSAPSPEHYG
                     ALKRELQQLYARDPEQAYLAAFERAASDPEASARLGLARLRYECGADLSVSEVEGGRI
                     RVTASAAL"
     gene            13967..14347
                     /locus_tag="VFQ61_34290"
     CDS             13967..14347
                     /locus_tag="VFQ61_34290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959629.1"
                     /translation="MSQLQAVEIPKVQGVTAEIIPSGQLKLSGTIAARDPTEGLAPFF
                     RNVHKAVVDGRLGQFKVDVSGLTFVNSSAIRLFVDWVTWVKNDVSSQYKLVFVTDSRV
                     TWQKTSLPVLKSLAPSIVIIEAAA"
     gene            14358..15083
                     /locus_tag="VFQ61_34295"
     CDS             14358..15083
                     /locus_tag="VFQ61_34295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959630.1"
                     /translation="MSGISRTSFVSTGRKELDKAFGGAAHREPARASLDKVSNTEPPR
                     AGAPTTGRPRGGDDMSRDGSLGARETHVLSSRNSFLPARSALEALKVNAVTLMEMRLE
                     ASPQMLDPTRAFVSSYVERRFHSRTAERVALACHELLENAVSYGSVSSDVVFALVQSG
                     QIIEVRVSNKASPGRQSMLRKHMEKLKVDPEKTFLEEMARSVSASGARAMLGLARVCH
                     EAKMDLEISLDGPTITTIARCGR"
     gene            complement(15266..17881)
                     /locus_tag="VFQ61_34300"
     CDS             complement(15266..17881)
                     /locus_tag="VFQ61_34300"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9959631.1"
                     /translation="MSTASGSYVLVVHAPQQAPAPREFASPRVVIGRETGDLVLPDAQ
                     CSSTHAELLFDGHSVRLRDLGSTNGTWCNGQRIQELAWALGVSIQIGAHYITLHEVKA
                     PVAARGRTAIAAAVPGFAYPGAPGGGQGPGPAPGAPQPGAPQPGPQGSFQQAAGGYGG
                     YSAPQANAGYAPQPMGYGQPGHGHSPPHAQGAYHPGTPYAAAAPTTPGQTQPGGKRKS
                     NALWIILPLFLVGTCGAGAAGLMWLGSRQPSPENGSASTATLDAPREATVKFVWFKGP
                     VGPSASGGTSPARVRVAPNHSGVVSVGVAEEFAGGGGNQWRTATWLAAFNATRTLGAS
                     LADYEFNVHVGGHTDGPSAGMLTTTTMLSLLRGKPLREDSTMTGTINPDGTAGPVGGI
                     VQKMRGAKEAGLKRFGFPIGTRNHKDLQTDQQVDLVNVASQLGLETREIGDVYDAYEF
                     MTGDKLPRVEPASDADLEPSEQTQALLRAKVSGWKVRVEREIAALKEEVARSGKAVEF
                     AAPLLAQAEKAYENAKRYESNSFLVPALDGYAETAVTIATATRFVKIVPHLFRHDVPA
                     LLEILESAKRVNGDVEAFGQELEVRAQSKTRGGQVSTTSAFTHYVTAKSATMIGDDFR
                     DNAVRLIQGIAAGNIAANNQALQAVLEQATLPILYYDIARVYLDYAKDVQGLIADEGE
                     RKPLAPQAVDRLVAGYASASAAVLAYFNALIVDEIASDAKMSAEQAKFAMAQREPTYY
                     LANKANMMSEYTGKTGATDGVKLMRLAAAGSAYLEGATLVNKWYSLGMNFDKSGELVL
                     DNRRALTAELELARRNAREAAARSKAAAGFIPTAARLAYQTANARREGSDEDKLAALA
                     AYWQSSFWSDLIAAQ"
BASE COUNT         3092 a         5908 c         5897 g         3394 t
ORIGIN      
        1 cgttgcgccc atcccgggaa ctcgggtcga ggcggagttg cttgatggcc acttcgcggc
       61 cggtgaacgt gtcgacgccc cgaaacacag ttcccattcc gccctgcccg atgcatgacc
      121 cgatttcgta gcgccctccg atgcggggca agttggcgtc gttggacatc gctcttgggt
      181 cttcagggcg gctccccggg ggcagtgatg cgctcggtgg cctctctctc gtggaaaaag
      241 cgcaaatcgt tgaaaggcgg aggccctgaa gcttgggctt ctcgaattcg gccctgcgcc
      301 gatcctacgc cctcgttgga ggcccgtgga ccttagcaga aatcagccac cctcgatccg
      361 tgtcctcgag atccgccgca caggacattt tcactgcgca gcgacgccag gcgatgacgc
      421 tgcccgtggg gactccagga cgttcgctcg agatctctgt ccggagctgc gttcgaaaac
      481 tctgctcgag aactctgctc gggactctgc tcgaaaactc tgcgcgatcg atcgctcgcg
      541 aaggttgcgc tccgacatgg ctcgggcgcg aggcgccgtg agctggtgtc gagattggcg
      601 cgaagaattc agagtgggac gacagccggc cgccccggga cctcgccctg accgggtcga
      661 agtccgcgtc cgttttgggc tcggccgcgt ccctccagtg gaaccttgca gggcggcccg
      721 gcgctctcgc gcggcacgtt ccggcgaagc cctcgatgtt gaccggcttg tgtacacatc
      781 gtgtttgaac attcggtgcc gggcaccagt tttttccgca tttcgtcctg gatcggatct
      841 tgctcaaagt ccgttgtgta gtgggcgcca gggttcgtga atgggggccc ctcgcttcaa
      901 tttccgagga gtacgcaatg ggacgcttgg accaagttcg ttcggtgaat gcagaaggcg
      961 ccaaggctgc gcaaacggtt tcttttcaaa ggcgctcgga cttggccgga gtcgagattc
     1021 ggagtgtgga aaacgctcaa gaagaagcgt ttttctacag tccggacttc gagttcttcg
     1081 tgccaatcac gtggcgcggt gaggtttggc accgtcgccg tcgcattcag ctcgagcccg
     1141 gctcgatctt gattgcacag cccggggagg ccttcttgtg ccgtcgcgcc caggaggcgg
     1201 gaaatcacat ctcgctcagc gtggaggctg aagtgatggc gcgcttcgcg gagctgtgcg
     1261 gcgtggctat ggctgacttc gcctttagtg cggatggtct cctgtccgac gcactttcgc
     1321 ggtgccttcg agccctaacg gactgtttca agtcgacggc cgcgaccgaa cccgtcgagg
     1381 tccatctcat ggagtggctc acgctcgtgt tccaacaact acgagtgggg tccgccgcgc
     1441 caccggccgg cgcctcgctg gagaggcccc ctgccgagcg cgtgcgtgag tgcttgcacg
     1501 aagacctgtc tgcgacactg gacatgagtc gggtgtcccg tgagacgggc ttgagccgct
     1561 ttcaagcact ccgtgccttc aagagctggt atggcctacc tccgtgcgcg taccaactgc
     1621 gggtgcgttt gggactggct cagcgtttgt tgcgtcacgg ggcgcgcccg gccgcggtcg
     1681 ccgcagagta cgggttcgtg gatcaaagcc atctgactcg gcactttcgg cgttggctcg
     1741 gaatcacccc cgccgagtac gctcgcgtgg gtatcgaacc gagtgcgctt cgcacgaacg
     1801 acttgagtct gctggcgggc tgaggggcat acgggggtgc cggtccgcgg ccggtggcgg
     1861 catgagctcg tgctctggag cgggcgcgat gtagtagagt cgctcgagct catgcccatt
     1921 caagccctga atcacttcac cgtgttgacc cgcgatctcg acgcgagcct gcacttctac
     1981 gtgcgtgtct tgggcttgac gattggggcc cgccccgagt tgccctccag cggcgcgtgg
     2041 ctctacttgg gcgaacaggc cgttttgcac gtgatcgtcg accctcgggc gccggagcgg
     2101 aacggagtca tcgatcacgt cgctttcacc ggcgtgaatt tgccggaggt aaccgcgcgg
     2161 ctacggtcgc acggaatcca gttcgagctc gtgcgtcagc cgggatccgg gacttggcag
     2221 ctgttcttgt tcgaaccgag cggcggcctc ttggaaatca atttcgacgc cagcgagtcg
     2281 gagggttgag ccttcccggg gtcgttgtta tttcagggct tggatggctt ggccgacact
     2341 cgccaccgtg acggccgggc ccgtcttcag ctcgggcgcg aagatcgcga cacgcagctc
     2401 ctcgagttgc cagcggacgc gggcgcgcgc ttctgacgcg cgagcgtcgg cgggagcccg
     2461 caatgcttga aactctctca ccagcgactc gatgggcgcg agcttctcgg catctttgcg
     2521 tggatcagcc aaggcgcgct gcacgcgcgt ttgcgcggcc ttcaaatagc gtggaatctg
     2581 cgccaagcgc gtgatctcca ggttactcag cgcgtccgca ggtaacagct gctcgagctg
     2641 ggcacgcacg ctgctcgcga ctgcgcgccc catgggttgc tcggcggctc gggccaagac
     2701 tcgctgcgtg gcctcgagtt ccccggcgat ggccaacacc gcatgattca aggtgttgaa
     2761 ggcttgcgcg atgcgcgggc tgccttcgcc cagcagctgc tcgaactgca cttggttcgt
     2821 cggcaaatgt tcgagtgttt gcagaccgaa ggcctgatcg aacactttgt tccagaactg
     2881 ctcctcgaag tcgggactgt tcagcatggt gcgcccgctc gtcggcgcga aaccgcgggg
     2941 gaccttggca ttgagcgacg ataaggaccg acgggtcacc aggcgtagga gccgccgcac
     3001 accggcccga gtggcgcgct ccgcctcact cgacgagtcg agcaacagca gatcgacatg
     3061 cgtgacgcga tccatcaagg cggggtagct cttgatcacg agcccaccac tgcgacgctc
     3121 cacgatcgcg ggtagctcac cgaaattcca ggcggtgagc ttcgtgcgtt ccaggttcga
     3181 aacgggagcg agcgcgcgca gctcctcacg tgctttcggc aggagctgct cgaatagcat
     3241 ggctagctcg cggctttgtc ccaatgcgtg acccgagtta tcgaccaccc gcagcgtcgg
     3301 gcgcaggaat ggtggcagcg tctcggggcg aaaggccgac tccggcacgt tcacgcaggc
     3361 tagctccgat actgtttgcg cgagctgcgc caggaagggc ccgcggaaag gagtcaagcg
     3421 ctccgcggcg cgcgcggcga gctcgttcac gttgcctagt tctttgcgca acgccttggg
     3481 gagcgcctcg atgagcgcgg tgatgcgttg caaatgccag cccgggattg tccagtctag
     3541 ttcacgcgga tcgaaggacg gtagcaggag cagcggaaca tccagggtca ggccgtcggt
     3601 ctccgcgctt gggtcgaaca gatagtgcag cgcaatgcgc tcgccggaga cctccacctg
     3661 gtctgggtag tgcgcctcgg tcaagccttg ttccgaggcc agcacgtcgg agagctgcag
     3721 gtgcaacgcc ttcggctgcg tccgctcgat ctcctctctg aaggactcga aggtcttgcc
     3781 gttcaccacg ctcgcgggca gtagccggtc gaagaacgcg aacagcgctt cgtcgtccgc
     3841 gagcatgtcg ctttggcgcg ctttgtcgcg caggcgcgcc acctcggcga ggagcgctcg
     3901 attgtgccct tcgaaggcac ccttggattt gtattccccg cggaccaggg cgtggtcgat
     3961 gaaccaacgt cgggcgtcac tgctggaaat agtggcgtag tcgacttggc gtcgagtggg
     4021 cacgggcagc ccgaacaagg tggaccgttc ctcgatcacg gtgcgtccgg acttctcgga
     4081 ccagtgtggc ttttcgtagc tacgcttgag caggtgggag cccactcgct cgaaccaatt
     4141 cggatcgatg cgcgccgcgt tgcgagcgaa tagttgggag gtgtgcacga gctcgaacgc
     4201 catcacccac gccgggggcc tcttggctag cgcggaactc gggtgcagga cgaagcgcgt
     4261 ctgcttggcg ccgacgtaca cgcgctcggt cgagttgtaa taaccgactt tggagagcaa
     4321 gccggagagc agggccatgt gtagcgcgtc actcgtatcg cccgcgttgg ctggcgagtc
     4381 cccgccggcg ccggcttgaa cttcggcttt ctttggcgaa gagtgaagcg cattgtcgcg
     4441 cccgcgcaaa ttcccattct ggctctcgcg agggcggccg ccatgttggg gactgcgatg
     4501 ttggcggtcg cgagctgaat tgtcgcgagc tgaattgtcg cgaggtcggg ggtcacgatg
     4561 tggggagtcg cgcccgcccg ggcgaagggg aaggcggagt tcttcggcga gctccaacag
     4621 ctgtctatgg atctcctgcc actcgcgcac gcggatgaag ttcaagaagc cgtccttgca
     4681 cgcgcgccgt agcccggctt tccccgcact gcgctcgacc tcgcgcaaga agtcccagag
     4741 cttcagccaa ccgacgaaat cggaacgttc gtcgcggaag cgttggtgtt gttgatcggc
     4801 cttttgctcc gcggcgcgcg ggcgttcgcg cggatcttga acgctcaacg ccgcggcgac
     4861 caccagcacc tcgggcaagc agccatactc agcgcccgcc aagatcatgc gtccaatgcg
     4921 cggatcgacg ggcaatcgcg cgaggcggcg gccggcgtcg gtcaactcgc gctcggcgtt
     4981 caacgcgccg agttcctgca aaactcgata gccttcggtg attgcgcgcg gggccggggg
     5041 atccaagaac ggaaactctt ccaccgcgcc gagccccagc gccttcatcc gcaagatcac
     5101 cgaggctagc ccaacgcgca gcacctctgg atccgtgaag gccggccgag ctcgaaagct
     5161 ttcctcgtcg tagaggcgca aacacagccc ttcgcgtacg cgcccggcgc ggcctttacg
     5221 ctggtccgcg ctggcctgcg aaatggcctc gagcgcgagg cgcgtggtgc cggtgcgcgg
     5281 atcgtagcgt gacaacctgg cgtagccggc gtccaccacg tatacgatac cgggcagcgt
     5341 gagcgaggtc tcggcaacgt tggtcgccag gatgatgcga cgctcgggcc cgctcgtgaa
     5401 cacacggcgc tgctctgccg aggacaggcg cccatacaag gggaggatcg ccgtgtgtcg
     5461 cagtttccgc ccctgaagct cgcgttctgc ctcgtggatc tctcgttcac caggcaggaa
     5521 aaccaaaaca tcccctcggt tgtcgagcga ggtcaggttc acgacggcgt tgccgaccgc
     5581 ctccgagaga tccagctctg ggtcaggggc ttcgtacagc acctcgaccg gataggtgcg
     5641 cccttcgacc tcgatgactt tcgcctgccc gaagaactcg gcgaagcgct gggtgtcgag
     5701 cgtggcggag ctgacgacca ccttcaagtc ggggcgttcg gacaagatcc gcttcaacca
     5761 cccgagtaga aagtcgatgt tcaggctgcg ctcgtgcgct tcatcgatga tcaacgtgtc
     5821 gtactgtcgc agcaaacgat ccgtctgagt ctccgccagc aggatcccgt cggtcatgaa
     5881 cttgacccga gtgcgcgagt ggctgcgatc ttcgaagcga atctgatatc caatctcctg
     5941 accgacgttc gtgccgagct cggaggcgac gcgctgggca atgctcgtgg cagcgatgcg
     6001 ccggggctgg gtgacgccaa tccggcgtct cgacccacgg cctagcgcga gcatcaactt
     6061 gggcagctgg gtggtctttc ccgagcccgt ggctcccgcc acaatcacga cctgggactc
     6121 cgctacggct cttccgatgt ctgcaactcg agccgagatc gggagttcag gcgggaactc
     6181 gatgcgcagc ccgttggata gggccgggcc cgctggtgcc gacgggggca tccgagctgg
     6241 gtctggttca gagccgtgct gttcagagcc gtgctgttcg gagccggatg gatctgtgag
     6301 cggctgggcg gggccgcgct ccgcgggaat gactggctcc ggacacatgc cggcccaggc
     6361 gtagcatcat tcctgacttt gtccgctagc tgcgcgacgc ggcgccttgc gcgatccagg
     6421 tgcgcagctc gcccaggagc tgcacgcgct ctggtgtcgg cagctccgcc cccgagctcg
     6481 cttccgcgag caataggcaa ggttgcaagc ccttcccgaa gcaaccgtag agcgcgaatg
     6541 caggcgaacc cagcacctcg tagtacgaga ctggaatgct cgcggtagct ttgggcgaga
     6601 aagcgtccgt gatcaccacg tcgcgccccg aatggtacgc cgagtaaaag acgctcacat
     6661 tgcgggtcgg atagatgggg aaccgaagtt cttttgcgac cgcctcgagg tcgtcccgga
     6721 agccgaagcg cacgctgagt tccgccttgt tgccggtcgt cgcgagcacg agcaagcgct
     6781 ctagccctag aatctccgac gcgagcttca gcgcggcgga tagcaagcgg tccccggaca
     6841 ggccagttcg cacttgggtt cgcagctgct cgaagcgcgc ttcccagcgt gtcatctgtt
     6901 ccggcgtcgg ggcgcgtccc gcgttctgcg gcttcgcgat ttgcaccggt accacgtgtt
     6961 ttggtgccgc ttccggctcc tcgccgccga gcaccgccac cccgggtggc gcgttgcccc
     7021 gcccgttcgg cgcgagggcg ccctccgggc tttgcaccgg cggcaccaac tgcgacacgc
     7081 ccgccagcaa acggctcttc ttcagctcgg ggccaagcac attggagtag cgatccaaga
     7141 acgcctgctc gacggacgcg aggcgctcta gcagctcgtc cggcgtgagc ttcaaaaagt
     7201 ctttgtactg cagcagtagc ttgccgagct tggctttgcg cgatggcgcg ggctcattgg
     7261 caatcagctc gcacacctcg gacgcgaact ccgacagcgc attcaaacga tcttcggcgc
     7321 gtacggggtt actgcccgcc gtcggcgcag aggcgatcgc cgtcaccatg cggctcggca
     7381 agtgccagct ctcggcgatc tccacgccga gccggccaaa cgacaatccg agcaccttct
     7441 ccgcagcgtg gcgcaaattg attcgctctt tggccatcac ctgcaagatt tggtcgtaca
     7501 tctccggcag gtagatcagc accaagtgcc ggccgaggtt gcggaacatg gcgcagacca
     7561 tggcctcctc gtcgttcaag gtcacatccc gcgcgagctg acgcgcgatc tccccgctga
     7621 ccagcgatcc gacggcggac tccgcgatcc gagcggcgtg cggatcttgg cccgcgctct
     7681 tgaacaggct gatgccgagc gccagcgaac ggatgcgatc gaaaccgagg atcacgatcg
     7741 cgtgctgaac gcggtagatc ttgcccccaa agcggttggc gtaggtggag ttgacgaccc
     7801 ggagcagctt ggccgtcagc gcgaagtcgt tgaggatcga ggccccgagt tggctcgccg
     7861 agtagatgct cgccgcgtcc gcgcgcttgc tcacttcacc tacgttctgg gagaacgccg
     7921 ggaagtcgcc tttgcgctgc atgcgcaagc ggagcttctt taccgtggat tgggcggttc
     7981 caccgagcgg tgtgtcgagc ggctcgggct ggctcggggg cgtgccctcc gactccggga
     8041 acgcgcgcga atccaccgaa acctcgtccg tttcgtggct cgtgtagcga ccggactggg
     8101 gctcgaccaa ttcgtgaata gccgcgtcgg gagccggtgg caccggaacg cgtccgacgc
     8161 tgctggacgg aggagggccc gcgcgcagcg cgatcttcat cgactgggtg aggtcgacga
     8221 tctcgcgcgc ttcgcgctct cgttctgccg gtgccgctcg gtcctgcaag cgccccagct
     8281 ctcgcgccag cgctgctgcg gagggcgggc gcgcctccgt atgcttcgac aagcagcgct
     8341 cgatgacctc ggcgagctcc tccggcacct ctggcacgtg ctcccgcaac ttgggcaccg
     8401 gatcacgaag gtgcattacg agcagcatca agtccgaagc atgctcgaac acgggcttgc
     8461 ccgtcagcag ctcgaacgca atcgctccca gcgcgtacaa atcgctgacg ggcgggatct
     8521 ggtggagttc gaggttggcc tgctccggcg cggcgtagcg cggtgagctc aggacctggg
     8581 ctcccgttcg tcccgccccc gagggcagca gcggcgtcga gccgaagcca ccgagcttga
     8641 ccgtcagccc gcccgcctcg tgaacgaaga cgtgctccgg gcgtaggtca cggtgaacga
     8701 gcccggcctc gtgtgcggcg cccaacgctt cacacaccgg gcccagatac cgtaccacct
     8761 cggccggggt caaacgcgat cgcgcccgca aagcagcccg caaactctga ccgcggccga
     8821 actccgacac atagtaagag cggccttcga cttcgccgat gtcgagcacc tcgagcaggt
     8881 tatggtgctg cagcttggcg agcgaacgcg cttccgacac gaagcgcttc gggaactccg
     8941 cgtccccttg cagctcggga gccaagaatc ggaccgcgac ctcgcggttc aaatgtacgt
     9001 gacgagccgc gaataccgag ccgctcgccg agctcgagag ggcccgctcg atgagataac
     9061 tcccgaggcg ccgcccgagc agcgacgggc catcgggaac gcgatcgcgc ggctgttgcg
     9121 cgggcgatag gtgccaagct tgaccgtttt gcgacatccg aagcgcgcga gtctggttcg
     9181 accctgctgt ttgtccttac ggtcggggcg ggccgataag cagccgaaaa gaggcacacc
     9241 ctcaagattt ctcggcgccg cgtgtgggcc ggactgcgtg tttggcagcc ggggaccgtg
     9301 gcccgcctcg agatccggtg agggtgtgca ctgctccctc tatgccgcca gagttagcga
     9361 gatcacgagc cgctctgcgg cgccaactcc gggcaacgct cgaaaggtcc cttgcaaact
     9421 tcgggaatct ccggctctgc ctcgaactcg gatagatcac tgtcacacgt caagctggag
     9481 taaaccgcga gcccttgctt ggtgtatgcc tcgttgcaat ccaagaactc cttgacggtc
     9541 acgttgtcac actgcggcaa ctcaccgctg caatcgcccg ccggatcctc cggctcttcc
     9601 tccgcgaggc attcggcctg agcgctctcg cagtcacctt gccccgcggc ctccatcagc
     9661 agccccgtga acgcacagat gccttgattc agcttactga cgtcgacgtc cttgaagcga
     9721 tcggcggcct tattgcagaa cgcctggagc tcggagtcgg tatcgatgtc ggacaagggt
     9781 gtgctatccg agagtggagt cgagacgccg cctccggtgc ccgtgcgtcc gcccgagctg
     9841 gctacaccgc cgctgccggt cttgccaccg gagctcgccg cgccacccgt gctcgtcttg
     9901 ccaccagtgc ccgtcttgcc accggtgctc gtcttgccgc cggtttcttc gccggaagtg
     9961 ccaccagtct cggggttttc cgaaccgctg ccggcttcgg tgttcgagtg gccccccgtc
    10021 gagctctttc cgcccgtgct tgctccggcg ttgccgcccg caccggacgc cggatcatcg
    10081 ctgccaccac aggcggccaa cagagaactg aaagcaatta aggaaactac caaagaacca
    10141 gcaatttttc gcatcgatca ctccgatggg cagctgctcg cgggagattc gttcatcacc
    10201 ctcggcagtg cgttcaagtt actggttctc gtacgccgcg catcctaagt tcttcctttc
    10261 gagctcttaa gatctcgcta cgacgagctc gcgcgtgcga tcgccccggc gttccgtcga
    10321 aagagttcac agggcacggc gccggccgtc tcgtttcgaa tcagctcgtg cctcgtgggc
    10381 aatcgattga gtgctgcgca cgcgcgtggc gttacgaggt gcggccggcg cggccacccc
    10441 gacgcacgcg tcgcgaccca agtgctcccg gcaccgggtc gcgactcgtc tcgcgctctt
    10501 tacggagcgc cgaccaactc atcagaactt caatccgacg ctgccgaaca cgctgaacgc
    10561 gtcgtattcg gagacgatca agtagcgcgc gtccgcgccc acgaatagcc cacccaacgg
    10621 gatgatggcg gcggcaccgg gccagactcc gaacttacta tccgtcgaac tctcgtcacc
    10681 cactttcgcc accccggtga cccgccccag cccgagatac ggccgcacga tcagcaaggg
    10741 aacggtgtcg atgtcgtagc ccgcctcgac accgtagtag tagacgttgg ccttcacgtc
    10801 gatgccggcg aggctctcgg tttttccgag gtgatagacg aacgtgccgc ccgcgtagag
    10861 cttgaaggga aacgtgtagc ccgcgcgcgc gccaatgccg accttcaacg cgtcgtcgaa
    10921 gccgtagccg ccgagcgcac ccgcgctgac ggagcctaga atatccgccg cggccgtgcg
    10981 cggccgcagt gaccccgcgg cgaagagcgc cgcccccaac acgagtctcg caagccaagc
    11041 atgcttcatg gttgaaacct ccagaggatg ctcgttgcta gcgccgcttg cagaatcctg
    11101 tcaagccagc cgaagccggc gtgcggagcg tgcgtctacg ttcggttcag gaggcgtatc
    11161 cgttgaggcg cgaagaggcg cgactcttgg acgcggagcg gcgcacatgc tgcggcgtgt
    11221 ggcgaaccaa gagcagggtc cagtcgtctt cccgccgggc cgcgtactcg gtgaccttcg
    11281 aaatcaccgc atccgcaatt tcttccagcg attgatagcg actcgaagca tcccgaatcg
    11341 ccgccaatag ccggggcgtg tcgaacaact caccttccaa gttgcgcgct tcggtcaggc
    11401 catcggagta caaacacaga atatccgagt cattgagctc gatggcggtg agggggattt
    11461 catccagctc gttctgaatg cccagccagg gccccggcac ttcgaaggtc tcgcacgtgg
    11521 cggaggcggc ccggaacacg atcggaaatt ggtgggctcc cgcgcacaag aagcgacctt
    11581 tgccctcgta cgcgagcagc tgcgccgtga cgtacttgct gtccttcagg cgattgccga
    11641 tgttctcgca caacaagctg ttgacgttgc gcagcgcgtg tccgggggag gtcttggcgt
    11701 ccgctcggaa ctgagaggcg aacgcggcct gggtcatcag catcaccaag ccggcgtcca
    11761 cgccgtggcc cgagacgtcg ccgatcgtga tccagagcgc gtgatcctcg ctgtggagca
    11821 cgtcgtaaaa gtccccgcca acctcgtcgg cgggcgccat gcgaccggcg aactcgaagt
    11881 ctggatgctc gggagacggt ggtagcagcg cgcgctgaat gctcgccgcg atttctagct
    11941 cgcgcgccat gcgcatccgt tccccgagct cggcgcgggc gcgcgcgatc tcgttgatca
    12001 tggcgttgaa ggagtgtccg aatgccccga gctcatcctc cgaagacacc tgcacgcgaa
    12061 cgttgaggtc gccttggccc accttgcgcg aagcctcgtg gagctcgcgg acgctgcggg
    12121 tcacgccgcg ggcgatgaaa ccggctagcg ttacgctgac gagcaacacg atgccgacca
    12181 agctgatact gccaatctcg gcgcgccggc tctcggcgat cttctccacc gcgacgctct
    12241 tgacctgagt atttagctgg cgcgagatgt cattcaaagt cttcagggtg ccgtcttgag
    12301 agcggagcca atccgcggga tccacggtga gctcatccgt ggtgctctcc agcgccacct
    12361 tgcgcagctc gctcgcgcgg agctgctcgt gcgttttgag ggcgcgttcg tagctcgcga
    12421 gctgctcggc ggaggccgag gtgcgaaaca cgtcttcgta gaccttccgc tcgctgagca
    12481 gggtaacgag tgagcgatag ctgccaggcg gaaatcgtcc gagcgcgaac acgtgggcca
    12541 acaagccttg ctcttggctg gcccgctcgg tgagctccaa taccgccacg agcgagttga
    12601 tgggccgcaa gagttcaccg ttgtcggtga gatccgtcaa agccgcggtg ctctggatca
    12661 aggctctgtt ggccgagcca tagcgctcga acaacacgtg gaagtccgtc tcgtctgtcg
    12721 caccgaggcg acctcggcgc agcgcttcga cggacttcct ggcctcgcgc aagtcgcgtc
    12781 ctagccgttc gggcatgccg acgctagagc tgccctgcag gaaagcgtcg agcttcttga
    12841 gcgctttgtc ggtcgcagag tactcccgct ccagattctc gggcccttgt gtcggcgcag
    12901 acgtcggcga ggatggcggg agagcggcgg gttgagcgaa atccggcagc tccgagtccc
    12961 ggggggctcg ggcatggcgt ttctgcccgg tcagccaaca gagctgggcg cgctcggtct
    13021 gcagtgcgtt gacgagctga ccaatcgcgt tggacaactg ggccaagtgc tcgatggtac
    13081 cgagcccttc cgccttctga acttgtaggc gcgcatcgcg caccagaatt gcagcgagca
    13141 ccaaggcccc caacacgggc agccctgcga gcaggcccag cttcagtcga atcgttacgc
    13201 tcgtcttggc ggacaacgct tctctcccgc ctcagcctca cggccccccc ggcgttaccc
    13261 ataagtctcg taacgcgcga cctggcgact tctcggcgtc gcgctcgcat acgggcatcc
    13321 accccgcgcc cggctcgggc accaatccgc tgaccaggat cactcgtgac taaattagag
    13381 cggcgcttcg ccgttgcatg ccatcgtgct gactcttgcc ggaatagaag aagaccaggc
    13441 ggagcaactg ggaggaatgc ctcgtggaat ctgatcactt gcccgcatcg atacgcgtcg
    13501 gtcggttcga gccgtccgtg tacgtgcgct ttcgtccggc ttggacgtac atcgaaggga
    13561 ttcgtgagtt cgggcgcttc ttttgcggcg caaccttcaa tgagcccgag gtagccgaac
    13621 gcgcccgcgt gatcatccaa gagaccttgg agaacgcggt gaagtactcg cagcgcaccg
    13681 cccacagtga gctcgaactc acgatcagca gtgacggtaa gcgcctcgag atctcggtgt
    13741 gcagcgcgcc ttctccggag cactacgggg ctttgaagcg agagctacag cagctgtacg
    13801 ctcgagaccc agaacaagcc tacttggctg cgttcgaacg agccgcgagt gaccccgaag
    13861 cgagcgcacg gctcggtttg gcgcgcctgc gctacgaatg cggtgctgat ctttcggtat
    13921 ccgaagtgga gggcggtcgc atccgagtga ccgcgagtgc ggcactatga gtcaactaca
    13981 agcagtcgaa attcccaaag tccaaggcgt caccgcggag atcatccctt cgggccagct
    14041 caagctgtcc ggtacgatcg ccgcgcgcga tcccaccgag ggcttggccc cgttcttccg
    14101 caatgtacac aaagccgtcg tggacgggcg gctcggccaa ttcaaggtcg acgtttccgg
    14161 tttgaccttc gtcaactcgt ccgcgattcg cttgttcgtc gactgggtga cgtgggtgaa
    14221 gaacgacgtg agctcgcaat acaagctcgt cttcgtgacg gactcgcgcg tgacctggca
    14281 aaagacgagc ttgcccgtgc tcaagtcgct cgcgccatcc atcgtcatca tcgaagcggc
    14341 ggcctgaggg gcgccggttg agcggaatat cccggacgag cttcgtgagc accggacgca
    14401 aagaactcga caaggcgttt ggcggggccg ctcaccgcga gccggcgcga gcttcattgg
    14461 acaaagtctc caataccgaa cctccacgcg cgggtgcacc cacgacgggg cgcccgcgcg
    14521 ggggtgacga catgtcgcgg gacggctcgc tcggcgcgcg cgagactcac gtcttgtctt
    14581 cacgtaactc ctttctgccg gcccgctctg cgctcgaagc gctgaaggtc aacgccgtca
    14641 cgctgatgga gatgcggctc gaagcgtcgc cgcaaatgct ggatcccact cgcgccttcg
    14701 tctcgtccta cgtagagcgg cgctttcatt cgcgcacggc cgagcgtgtc gcgttggcct
    14761 gtcacgaact cctcgagaac gccgtgagct acggctccgt ctcgagcgat gtcgtgttcg
    14821 cgctcgtgca atcaggccaa atcatcgagg tgcgggtctc caacaaggcc tctcccgggc
    14881 ggcagtccat gttgcgcaag cacatggaga aattgaaggt cgatcccgag aagacatttc
    14941 tggaagagat ggctcgctcg gtgagcgcca gcggcgcgcg cgccatgctg gggctcgccc
    15001 gggtgtgtca cgaagccaag atggatctcg agatctcgct cgacggcccc acgatcacca
    15061 ccatcgcgcg ctgcggtcgc taaacgcatc ctcgatcccg ctccgggatc gagcgtcgtg
    15121 cgctgcgggc ccgggccgat ttgtaacgag cgcgaagcgc gtcgcgtcgc gaccctggcc
    15181 ccgcccgaac ccggcccatt cgccccatga accgcgccgg cccccgcggt cacgtccacg
    15241 caagggccca aagctcggcc gcccgtcact gggcggcgat caggtcactc caaaacgaag
    15301 actgccaata ggccgccaac gcggcgagct tgtcctcgtc gctgccctcg cgccgcgcgt
    15361 tggcagtttg atacgcaagg cgcgccgcgg tcggaatgaa ccccgcggcc gccttggagc
    15421 gtgcggccgc ttcccgagcg ttgcgtcttg cgagctcgag ctcggcggtc aaggcgcgtc
    15481 gattgtcgag cacgagctcg cccgacttgt cgaagttcat gccgagcgag taccacttgt
    15541 tgaccagggt cgcgccttcc aggtatgcgg agccagccgc cgcgagccgc atcaacttga
    15601 cgccgtcggt cgcgcccgtc ttgccggtgt actcggacat catgttggct ttgttggcca
    15661 ggtagtaggt gggctcgcgc tgggccatcg cgaacttggc ctgctccgcg ctcatcttcg
    15721 cgtcgctcgc gatctcgtcg acgatcaagg cgttgaagta ggcgagcacc gcggccgagg
    15781 cggaggcgta tccggccacc agtcgatcta cggcttgcgg cgcgagcggc ttgcgttcgc
    15841 cctcgtctgc gatcaagccc tgcacgtctt tggcgtagtc caggtaaacg cgggcgatgt
    15901 cgtaatacag gattggcagc gtggcttgct cgagcacggc ctgcagcgct tgattgttgg
    15961 ccgcgatgtt gccggccgcg atgccttgta tcaagcgcac ggcgttgtcg cgaaaatcgt
    16021 cgccgatcat cgtggctgat ttggccgtga catagtgggt gaaagcggac gtggtgctga
    16081 cttgcccacc gcgagtcttg ctttgcgctc tcacctcgag ctcttgcccg aaggcttcga
    16141 cgtcgccgtt cacgcgcttg gccgactcca ggatttcgag cagcgccggc acatcgtgcc
    16201 gaaagagatg aggcacgatc ttcacgaagc gggtggcggt ggcaatcgtg accgcggtct
    16261 cggcatagcc atccagcgcc ggaacgagga agctgttgct ctcgtagcgc ttggcatttt
    16321 cataagcctt ctcggcctgc gccagcagcg gggcggcgaa ctcgacggcc ttcccgctgc
    16381 gcgctacctc ctctttcaag gccgcgattt cacgctcgac ccgcactttc cagccgctca
    16441 ccttggcgcg cagcaaagct tgggtctgtt ccgacggttc gagatccgcg tccgaggcgg
    16501 gctcgactcg cggcagcttg tcaccggtca tgaactcgta agcgtcgtag acgtcgccaa
    16561 tctcgcgcgt ttccaggccg agttggctcg cgacgttcac caaatccacc tgctgatcgg
    16621 tctgcagatc cttgtggttg cgggtcccaa tcgggaaacc gaagcgcttc aaaccggctt
    16681 ccttggcgcc gcgcatcttc tgcacgatcc cgccgactgg ccccgcagtg ccatcggggt
    16741 tgatggtgcc ggtcatggtc gagtcttcgc gcaggggctt gccgcgtagc aatgacagca
    16801 tcgtggtggt ggtgagcatg cccgcggagg ggccatcggt gtgtccgccc acgtgaacat
    16861 tgaattcgta gtctgcgagt gaggcgccga gcgtgcgggt tgcgttgaac gcggcaagcc
    16921 aagtggcggt gcgccattga ttgcctcctc ctccggcaaa ctcctcggct acgcccaccg
    16981 aaaccacgcc cgagtgattg ggcgcaacgc gcacacgcgc cggcgacgtg ccgccgctcg
    17041 cgctcggtcc tacggggccc ttgaaccaca cgaacttgac cgtcgcctct cgaggggcat
    17101 cgagcgtggc ggtcgaggcg ctgccgttct cggggctagg ttgacgcgag ccgagccaca
    17161 tcaggcccgc cgcaccggcg ccgcaggtcc ccaccaagaa cagaggcaga atgatccaga
    17221 gcgcattcga tttgcgtttg ccacccggtt gcgtttgccc cggcgtggtg ggggcggcgg
    17281 ccgcgtacgg cgttccgggg tgataagcgc cctgagcgtg cggcggtgag tgaccgtgtc
    17341 ccggttgacc atagcccatg ggttgcggcg catagccggc gttcgcttga ggtgccgagt
    17401 agccgccgta gccaccggct gcttgctgaa acgatccctg cggcccgggt tgcggagcgc
    17461 cgggttgcgg agcgcccggc gcggggcccg ggccttgccc gccgccgggt gcgccgggat
    17521 aagcaaagcc ggggaccgcc gccgcgatcg cggtgcgacc gcgggctgcg accggagctt
    17581 tcacttcgtg cagcgtgatg tagtgagctc cgatttgaat cgatacaccc agtgcccagg
    17641 cgagctcctg aattcgctgg ccgttgcacc aagtgccgtt cgtgcttccg agatcccgca
    17701 agcgcacgct gtggccgtcg aacagcaact ccgcatgagt agaggagcac tgcgcgtccg
    17761 gcagcacgag gtcccccgtt tcacggccaa tcaccacgcg cggcgacgca aattcacgcg
    17821 gcgcgggcgc ctgctgggga gcgtgaacga caagcacgta agatccggaa gcagtcgaca
    17881 tgaattctcc gagcccaact ccgtgagcct gcagcgaggc acgtgaatcg ggaggctcag
    17941 tgtactggtt tcgaacgcga gcctagccgc tttttcaatg ggaattcgtt cgacggagtg
    18001 cgcgcgagac gcgcgcagag cagcctcgga cgcgcgcggg gtgttctcat gtcgcgaggc
    18061 cggtgtgttt cggtcacacc tcgcgttgcc gtactaccgg gctgacttct ctagccgcgt
    18121 ggtagtgcga gcaagtgggc tctgtgtccg gatgcagctc tgcgttcgaa tgcaactctg
    18181 tcgttgcagc aacgagagcc cacccttcgc cccgctcgag cacggagccc ggattgtgga
    18241 tccaggctcc gcagggcgca gttcacggat cgagcgtgcg aatcgagccg t
//
LOCUS       DASTKU010000966         4512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1531194, whole genome shotgun sequence.
ACCESSION   DASTKU010000966 DASTKU010000000
VERSION     DASTKU010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531194"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..480)
                     /locus_tag="VFQ61_34305"
     CDS             complement(121..480)
                     /locus_tag="VFQ61_34305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959632.1"
                     /translation="MQALAVRPQGYDAGAYRLVIRLSPDEQEIVRTAPFIAFELPRLP
                     NRTITPDAGFAVATNDSGVIRLRGVLRPDSGWLGHCHSNGVEESANPTPIGTIESALQ
                     QSAEGALASMRAWLNLG"
     gene            complement(553..2118)
                     /locus_tag="VFQ61_34310"
     CDS             complement(553..2118)
                     /locus_tag="VFQ61_34310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959633.1"
                     /translation="MMARSLENRAAARRELTLRRARKDLAAFTEYVFGLRLAPHHRAW
                     ALALSEAPRLVLMAPVEHGKTTIASVALPLWVLGNAPDSRIALVSETHTQAARPLAAI
                     REHILRNPRVREVFPNLRPARGARERWSDSEVVVERPSASKDPSVVAVGVSGPLLGAR
                     LDLAILDDVCSWENSFTSGQREKVIAWFRSTLVGRVVAEGRVVVVGTPWHREDLLHVL
                     EDGSEYRVQRDPAMNHAGEPLWPEAWPLERLEQRRREIGEIEFSRQMLLTVLAEAGAR
                     FREAWVEQAFAAASAVGCRMMETYADAAPTFTGVDLAVGSTVHHDESALFTVALLPDG
                     RRQVLSIEAGRWQAPEIVARIRSTRLRYRSRVRVESNAAQAYIAQFLAADGVHVDAHM
                     TGRNRHDPVFGIESLAVELEQARWIVPDAPETRTWARELLAFSPAAHPGDRLVASWLA
                     REAAREYEARPRVSPFFAESEFRQDEDEDSAFWVLGPSAGRPLTAEDRSPSPPSVLQP
                     RAPERRQRPFILR"
     gene            complement(2075..2533)
                     /locus_tag="VFQ61_34315"
     CDS             complement(2075..2533)
                     /locus_tag="VFQ61_34315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959634.1"
                     /translation="MANRLSSSNRAALWRAYQESQNANEVARKCGVHHRTVERYRVLD
                     RWDERLAEIRQEAQKRADYTLADAMAESLSLVRRYKEKLGRALDAKAVNGDDVSAAEL
                     ERVIRLEAFVLGGSESRHEIVTEFTSWSDEDLEAYARDGTVPRESRSRSA"
     gene            complement(2862..3731)
                     /locus_tag="VFQ61_34320"
     CDS             complement(2862..3731)
                     /locus_tag="VFQ61_34320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959635.1"
                     /translation="MQENAVGAIRSDNVLAGLFTMAVLFAASACSTVFLQNDGFRSKL
                     AGGCQTEDACSTLVDEASERIDHCKDNTIGYVRCQDATADLDEAKALLAKVKGSSGSE
                     SVKNRASTEPSQAPAMARAKADDPTREDMLNEMNERTAAQQATEHQQQVDLQAETQSG
                     RCTDAHAEQLRRVLDQVVGFWRDEPLQVVTHKIVVLTAAGIDLKMQIVLPGEYHVFAM
                     QPDGRAPELAVYDADGYEVKQASEWSGYFSAGGTGVGGRSRRLQWTDPSQPMLLKAAG
                     QGCAMIVLVQRLW"
     gene            3764..4084
                     /locus_tag="VFQ61_34325"
     CDS             3764..4084
                     /locus_tag="VFQ61_34325"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9959636.1"
                     /translation="MEHRCSSFRKLLRALLGAVTREDPAALLKNIGLRIAELRGKRGW
                     SREALAERLGVSVRYVGRLETGGQNLTVHRLAWLAGPLGVRVADLFAAPGIDRIRVGR
                     PARK"
     gene            4155..>4512
                     /locus_tag="VFQ61_34330"
     CDS             4155..>4512
                     /locus_tag="VFQ61_34330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET9959637.1"
                     /translation="MLKDMETERTKVIGYVRVSTEQQADGGVSLQAQRDKLHAYAVAM
                     DLDLVGVLEDAGASAKTLARPALQEALRRLETGEVDGLLVCKLDRLTRSVRDLGHLLE
                     RYFAARFSLLSVSDSID"
BASE COUNT          783 a         1523 c         1390 g          816 t
ORIGIN      
        1 acttggcgtg aatgagcaac agatcgaggc tatgcggcgc gctctcggcc gaccgcctcg
       61 gcgcccttct gcaatgtcag acgctgtcgc cctctccagg gccgcacgcc gcccgctccg
      121 ctagcccagg ttcagccacg cgcgcatgga ggccagcgcg ccctcagcgc tctgttgaag
      181 agctgattcg atcgttccga ttggcgtcgg gttggccgat tcctcgaccc cgttcgagtg
      241 gcagtgcccg agccagcccg agtctggtcg aagtacgccg cgcagacgta tgaccccgct
      301 gtcgttcgtc gcgaccgcga aacccgcgtc cggtgtgatc gtgcggttcg gcaaccgagg
      361 aagctcgaaa gcaatgaatg gcgccgtgcg cacgatctcc tgctcatccg gcgacagccg
      421 aatgacgagc ctataggctc ccgcgtcgta tccctgtgga cgcaccgcta acgcctgcat
      481 tgccgtcacg atacttggtc aggagcctcg gtcaagtcgc gcgtcgcatg ggagttcgcc
      541 gagggggaca cttcatcgga gaatgaacgg ccgctgtctt cgttctggcg cgcgcggctg
      601 gagaacggat ggtggcgaag gcgatcggtc ctcggcggtg agcggtcgcc cggcgctcgg
      661 tccgagcacc cagaacgccg agtcctcgtc ttcgtcttgc cggaactcgc tctccgcgaa
      721 aaacggcgac acgcgcgggc gtgcctcata ctcgcgcgcc gcctccctcg cgagccacga
      781 agcgacgagc cgatcgcccg gatgcgccgc gggtgagaag gccaggagct ctcgcgccca
      841 tgttcgggtc tcgggcgcgt caggaacgat ccatcgcgcc tgttcgagct cgaccgctag
      901 cgactcgatc ccgaacacgg gatcatggcg gttacgaccg gtcatgtgcg catcgacgtg
      961 gacaccgtcc gcggcgagga actgagcgat ataagcctga gccgcgttgc tctcaacccg
     1021 cacgcggcta cggtaccgaa ggcgcgtaga cctgatcctt gcgacgatct ccggagcttg
     1081 ccagcggcct gcttcaatgc tcagcacctg ccgacggccg tcaggcaaaa gggccacagt
     1141 aaacagcgcc gactcgtcgt ggtggactgt cgagccgacc gccagatcga cgccagtgaa
     1201 tgtcggagcc gcgtcagcgt acgtctccat catccgacag ccgaccgcgc tcgcagcagc
     1261 aaaagcttgc tctacccacg cctcgcggaa tcgcgcacca gcctccgcga gcaccgtcaa
     1321 gagcatctgg cggctgaact cgatctcccc gatctcccgt cgccgctgct cgagacgttc
     1381 gagcggccac gcctcgggcc aaagcggctc gccagcgtgg ttcatggccg gatcgcgctg
     1441 cacacggtac tcgctgccgt cctcaagcac gtgcaagaga tcctcccgat gccatggagt
     1501 tccgacaacg accacgcgcc cctcggcaac gacgcggccg accagcgtcg agcggaacca
     1561 cgcgatcacc ttttcgcgtt gtccggaagt aaatgaattt tcccaggaac aaacgtcgtc
     1621 caggatcgca aggtcgagac gtgcgccaag aagcgggccg ctaacgccca ccgcgactac
     1681 ggacggatcc ttcgatgcgc tcgggcgttc tacaacgacc tccgaatcag accaccgttc
     1741 gcgggcgcca cgcgccggac gcaggttcgg gaatacctcg cgaacgcgcg gatttcggag
     1801 gatgtgctca cggatagcag cgagcgggcg cgccgcctgt gtatgtgtct ccgacacgag
     1861 agcgatgcga gagtccggcg cgttcccgag cacccacagc ggcagagcca cggaggcgat
     1921 cgtggtcttg ccgtgttcca cgggcgccat gagcacgagc ctcggggcct ccgagagggc
     1981 cagagcccaa gcgcggtggt gcggcgcaag cctcaagccg aacacgtact ccgtgaacgc
     2041 cgcgagatcc ttccgtgcgc ggcgcagcgt aagctcacgc cgagcggctg cgcgattctc
     2101 gagggaccgt gccatcacgc gcgtacgcct ccaggtcttc gtcggaccag ctcgtaaact
     2161 cagtcacaat ctcgtggcga ctttccgagc caccgagcac gaatgcctcg agccggatca
     2221 cgcgttcgag ctcggcagca ctcacgtcat ccccgttcac ggccttcgcg tcgagcgctc
     2281 gaccaagctt ttctttgtac cggcgtacga gcgacaggct ctcggccatc gcgtctgcga
     2341 gcgtgtaatc ggcgcgcttc tgagcttcct gacggatctc cgccagccgc tcatcccagc
     2401 ggtccaatac ccggtaccgc tccacggtgc gatgatgaac gccgcactta cgcgccacct
     2461 cgttggcatt ttggctctcc tgataggccc tccacagggc cgctcggttg ctcgacgaca
     2521 ggcgattggc catccaccga acatcctacc tcatccgccg caaaggcaat ccgagctcgt
     2581 cgcgggtgcg cgcaatcgat tgcgtagaga gcgcgcgccg tgcctacgtt cgcgcaccgc
     2641 atcgctggca gcgccaaggg ttggcggagt agttatgcgt gtagcgcacc accagccata
     2701 cgccgcacgt cgcaagtacc atcacgaaat ctccgcaccc ccagatcatc gagtttcgag
     2761 tcgcgggaac gttggcttgg caggctcgac agtagcgctg actgacttcg ggtgctccgc
     2821 gtgccatttg atcctcccgt ttggttgtcg tcgccgcgca gtcaccacag gcgctggacg
     2881 agcacgatca tcgcacaccc ttggcccgca gctttgagta gcatcggctg gctcggatcc
     2941 gtccattgca gccgtcgcga ccgcccaccc acgcccgtac cgcccgccga gaagtagccg
     3001 ctccattcgg atgcttgctt cacttcgtat ccgtccgcgt cgtagaccgc cagctcgggg
     3061 gcgcgaccat ccggctgcat cgcgaagaca tggtactcac caggaagtac gatctgcatc
     3121 tttagatcaa tgccggcagc cgtgagcacc acgatcttgt gcgtgacgac ctggagcggc
     3181 tcatcacgcc agaagccaac gacttgatcg agaacgcgcc gcagctgctc agcgtgggcg
     3241 tccgtacaac gcccggactg tgtctctgcc tgaaggtcta cttgttgctg gtgctcggtg
     3301 gcttgctgcg ccgccgtgcg ctcgttcatt tcgttcagca tgtcctctcg cgttggatcg
     3361 tcggctttgg ctcgtgccat tgctggagcc tgcgacggtt cggtgctcgc ccgattcttc
     3421 actgactctg agcccgacga gcccttgacc ttcgcgagca gagccttcgc ctcatcaagg
     3481 tcggctgttg catcctggca acgcacgtag ccgatcgtgt tgtccttgca gtggtcgatc
     3541 cgttccgacg cctcgtccac cagcgtgctg cacgcatctt cggtttggca gccgccagct
     3601 agcttcgatc ggaacccatc gttttgtagg aagacggtgc tgcatgcgct cgcagcaaaa
     3661 aggacggcca tggtgaagag cccagccagc acgttgtcag aacgaattgc gcctaccgcg
     3721 ttctcctgca tcgtcgttac ctcgcacccg gggggaccct ccaatggaac acaggtgttc
     3781 ctcgtttcgc aagcttctcc gagccttgct cggggcggtg acgcgcgaag accccgccgc
     3841 tctgctcaag aatattgggc tccgtatcgc agaactacgc ggaaagcgcg gctggtcgcg
     3901 cgaggcgctt gcggagcgct tgggtgtgag cgttcgctac gtggggcgac tcgagacggg
     3961 gggccagaac ctaactgttc accgtctcgc ttggctggcc ggaccgctcg gcgttcgggt
     4021 cgccgacctg ttcgccgcac cggggatcga tcggatccga gtgggtcgac ctgcgcggaa
     4081 gtaggtctcc gccgcgggtc attgcctgtc acacccatgc gtcaacaaga cgagcgtttc
     4141 gtggtcagcg atccatgctg aaagacatgg agacggagcg caccaaggtc atcgggtacg
     4201 ttcgagtcag caccgagcag caagccgacg gcggcgtgag tctccaggcg cagcgggaca
     4261 agctccacgc gtacgcggtc gcgatggacc tggatttggt gggcgtgctc gaagacgcag
     4321 gggcgtcagc gaagaccttg gcccggcccg cgctccagga ggcgctgcgt cgcctcgaga
     4381 ccggcgaggt tgacggtctg ctggtctgca agctggaccg cctcacgagg agcgttcgcg
     4441 acctgggtca tctccttgag aggtacttcg cagcccgctt ctcgctcctc tccgtctcgg
     4501 actcgatcga ca
//
LOCUS       DASTKU010000967         5753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1082602, whole genome shotgun sequence.
ACCESSION   DASTKU010000967 DASTKU010000000
VERSION     DASTKU010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082602"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..442
                     /locus_tag="VFQ61_34335"
     CDS             173..442
                     /locus_tag="VFQ61_34335"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9959638.1"
                     /translation="MKLSIDNDVGEIDALERLAAFSASLVGQVGVERAQQCIKAAMLE
                     AALERTGGNYGQTARLLDVTRQAVQQMVIRFNLRTWVEERRRDAH"
     gene            complement(573..2069)
                     /locus_tag="VFQ61_34340"
     CDS             complement(573..2069)
                     /locus_tag="VFQ61_34340"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9959639.1"
                     /translation="MSRKQRDPVRKGPMYGGHPRLFRQTAAVDERFGTISSDLLRSGM
                     RQSFSTQRASSTLRLHADARQSGAVGRVHRASPHSQGTALFFQHTASSTASRVTLPKL
                     GRRCVFLNVRPLGASKPCDPDLSRANMLLQEIRDSRNKGARLGSSSGCFVRSSGAKGI
                     DGNDARRDARVSPRRVCGVGLRGKTTPLSIGARMGLLRHATSARRSYLEPDHLIGRAP
                     ESCLLIDEDYISRRHAVIRWVDDRWEIQDLGSRNKTYLNGEPLTPSVARPLCRGVTLS
                     FGHPDQLWRVEDEEPPRLMAVPREGGEPIWAEGDLLVIPSPSDPAASVYTDSRGIWVL
                     ESLDSVRPVNHEERIEVSGRGYRLCIPHVIQRTSAADAVGPQRMRDVRLLLSTSRDEE
                     HIRLTAELKGRSVDLGRRAHNMLLLVLARRRLEDVNGGFSAAEQGWMYQDEVLKALNI
                     DAQQLNTDVHRVRRHVTPMGVLDPVNIIERRTGQLRIGTSQLQIQDEA"
     gene            2178..3581
                     /gene="pyk"
                     /locus_tag="VFQ61_34345"
     CDS             2178..3581
                     /gene="pyk"
                     /locus_tag="VFQ61_34345"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET9959640.1"
                     /translation="MIARRAKIVCTIGPAVDTREGIRGLIEAGMDVARLNFSHGKHAE
                     HGARFDLIRAESEALGKPVAILQDLCGPKIRTGTKGPAVLETGGTIDLVAGKDGDDRT
                     VAVDYESLAHDVRPDDRILLSDGQVELRVLDVKGERVCCRVEHGGPMRPKMGVNLPSG
                     SLRLAALTEKDKADLEFGLEKGVDYVALSFVRRAEEVLELRELCEKKGRPTPIIAKIE
                     TPNAVERLDSIVRAADGAMVARGDLGVELPPETVPVIQKQIIGTCRIHRKPVIVATEM
                     LQSMVDSPRPTRAEASDVAHAVFTGADAVMLSAESATGKFPHAAVRMMDRIIRQAEGS
                     RFFLPNPSEPDHTTADSIAHASVAIAREVGAKLIIALTETGVTARLVSKARSSVPILA
                     FSSGERTLRQLALFWGVAPRALVAPKENFEDQVRDTTQYLLQNGLVKSGERYVMVYGA
                     RVGVRGATNSVRVEQLP"
     gene            3681..5483
                     /locus_tag="VFQ61_34350"
     CDS             3681..5483
                     /locus_tag="VFQ61_34350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9959641.1"
                     /translation="MEEIGHGGMATVYRARDRRLGRDVAVKVIHHHLRENREVAQRFE
                     SEARAVAKLKHPNIVEVYDVSDAQDDERYLVVELVRGTTLRKLLAAEGHLPPEVAAAV
                     GIELADALEHAHGMGVIHRDIKPENVLIRVTVPDSERERSRVKLTDFGIAKLLDAQGV
                     TSTGQVLGSPAHMAPEQIEGGEVTARADVFGLGVLLYECMVGRLPFDGKNPAQVLRRV
                     LDGAFTPPERARPTVGATYSAVLARALAHEPKDRYDSTSALAHDLRAELLAMGIEQPA
                     LELHQFLEAPAAYRAAEAARVVERLRKNAEGLRGQGKVVEAAACFNRALAYRPNDGEL
                     LRALSRLNRGARTRRVLLRGASTIAGVLGVAGAVVAVARMQTTPKREAKADVPTALVR
                     SAEPIPVSSFAVVSSSARAQPSSPESPPDSAPSGAPTARKNPRSGSSKPAPASSAEGA
                     PLAHVQILVDGPQSAIVRVDGIETSWFGRLRSMPPGPHTFEFVAPDETCCEPGQTLKV
                     NIPVPVNENDVTTVRGRIEFRPAMLELSGPAGSLASCGALGEFPVPGQRQIPMAGAVR
                     RVRCQLIPPPGSAEPPKEFDVTLRAGRLSTNLGQ"
     gene            5509..>5753
                     /locus_tag="VFQ61_34355"
     CDS             5509..>5753
                     /locus_tag="VFQ61_34355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959642.1"
                     /translation="MRPVNWFFVLLITYGSPAMAQSRGATSAPRAPAQASDAGLNDET
                     ELTRVVSLFEAGKYRQCAEELKGLLSDEGTRRLRDRD"
BASE COUNT         1019 a         1752 c         1928 g         1054 t
ORIGIN      
        1 gagttccacg cggtgctccg ctcgttgacc gcactgtgcg gactcggctt tccgcgttgt
       61 ctaaccgaca tagcagtccg gattgtcagg cattgcaatt gaccccggcg gctagttgcc
      121 gccttttgtg gccatgaaag ccagggaaaa tggcgctcta aaggagcgtg gggtgaaact
      181 ttccattgac aacgatgttg gggaaatcga cgcactggaa cgcttggccg ccttctcggc
      241 ctcgctcgtg gggcaagtgg gtgtagaacg agcgcaacag tgcatcaagg cagccatgtt
      301 ggaggcagct ttggagcgga cgggtggcaa ctacgggcag accgcgcgtt tgctcgatgt
      361 gactcgtcag gcggttcaac agatggtgat tcgcttcaac ctgcgaacgt gggttgaaga
      421 gcgccgtcgt gacgcccact gaaaatcgac ctaggccaat caacggatcc gtactggatc
      481 gaggcagaac caatcagggc agtgggggag tctccgagtg tcgtgggtgc tcgggctcct
      541 ccggcctttt tctgcgttcc tacgcccgaa attcaggctt cgtcttgaat ctggagttgg
      601 ctggtcccga tgcgcagctg acctgtgcgt cgttcgatga tgttcaccgg atcgaggacg
      661 cccatgggcg tgacgtggcg tctcacgcga tgcacgtcgg tgttgagctg ctgggcgtcg
      721 atgttcaggg ccttcaagac ctcgtcttga tacatccagc cttgctcggc ggccgagaag
      781 ccgccattga cgtcttccaa tcgtcttcgc gctagcacca gtaacagcat gttgtgcgcc
      841 cgcctcccga gatccacgct gcgccccttc agctcggccg tcagccggat gtgctcctcg
      901 tcccgtgacg tggaaagcag caagcgcacg tcccgcatgc gttggggccc gaccgcgtcg
      961 gcagcggagg ttctctggat cacgtgcggg atgcacagcc ggtagccgcg tcccgaaact
     1021 tcgatgcgct cttcgtgatt gaccggacgc acggagtcta ggctctccag cacccagatg
     1081 ccgcgtgaat cggtgtacac cgaggcggcc ggatcactcg gtgaagggat gacgagcaag
     1141 tctccctcag cccaaatggg ctccccaccc tcgcgcggaa cggccatcaa gcggggaggc
     1201 tcctcgtctt cgacgcgcca cagctggtcc gggtgaccga aggacagagt gactccgcgg
     1261 cacagcggtc tcgctaccga tggagtgagt ggctcgccgt tcagataagt cttgtttcgg
     1321 ctgccgaggt cttggatttc ccaacgatca tcgacccagc gaatcacggc gtgccgccgc
     1381 gatatgtagt cttcgtcgat gaggaggcag ctctctggag ctcgcccgat tagatggtct
     1441 ggctcaagat agcttcttcg cgcggaagtt gcgtggcgaa gcaggcccat gcgtgctccg
     1501 atgctaagcg gtgtcgtctt gccgcgcaag ccaacgccgc aaacgcgtcg tggcgaaacc
     1561 cgtgcgtcgc gcctcgcgtc gttgccgtca atgccttttg cgcctgagct ccggacgaag
     1621 cagccgctgc tggatcccaa gcgcgcgcct ttgtttcgag agtcgcgaat ctcttgcagc
     1681 agcatgtttg cgcgcgacag atctgggtcg cacggcttcg atgcaccgag cggacgaaca
     1741 ttcaaaaaga cgcagcgccg cccgagcttc ggaagagtga cacgtgatgc agtcgacgat
     1801 gcagtatgtt gaaagaaaag cgccgtccct tgcgaatgcg gactggcgcg atggactctc
     1861 cccactgctc ctgattgccg cgcgtctgcg tgaagccgca acgtggagct tgcgcgttgc
     1921 gtcgaaaagg attgacgcat acctgaccgc aacaaatccg acgaaattgt gccaaatcgt
     1981 tcgtccaccg cggcagtttg tcggaacaga cgcgggtggc cgccgtacat cgggcctttc
     2041 cgtaccggat cacgctgttt gcgcgacatc ggggcctacc cttttggtcg tgccatgtgg
     2101 catgctgcgc ggcacacttt gccctgctgg agcttcgacc gaaaagcggt tcgcgctctc
     2161 cctcacggaa cgattacatg attgctcgtc gcgccaaaat tgtctgtacc atcggtcccg
     2221 ctgtcgacac acgcgaagga attcgaggct tgatcgaagc cgggatggat gtcgcccggc
     2281 tcaacttctc acacggaaag cacgccgagc acggcgcgcg cttcgacctg attcgggccg
     2341 aaagcgaagc gctcggcaag cccgtcgcga ttctgcagga cttgtgtggt ccgaagattc
     2401 gtaccggcac caagggcccc gccgtgctcg aaacgggggg cacgatcgat ttggtggcgg
     2461 gcaaggatgg tgacgaccgc accgttgcgg tcgactacga aagcctggcc cacgacgtgc
     2521 gtcccgacga tcgaatcctg ctgtccgacg ggcaggtgga gctgcgcgtg ctcgacgtca
     2581 aaggggagcg ggtttgctgc cgagtggagc acggcggccc gatgcgcccg aagatgggcg
     2641 tgaacttgcc ttccggcagc ctgcggctcg cagctttgac cgagaaggac aaggccgatt
     2701 tggagttcgg gctcgagaag ggcgtcgatt acgtggctct ctcgttcgtg cgccgcgcgg
     2761 aagaggtgct cgagctgcga gaactgtgcg agaagaaggg gcgtcccacg ccaatcatcg
     2821 ccaagatcga aacgccgaat gccgtcgagc ggctcgactc gatcgtgcgc gcggccgacg
     2881 gggccatggt ggctcgcggc gacttgggcg tcgagctgcc gccagagacc gtgcccgtga
     2941 ttcagaagca aatcatcggc acgtgccgca tccatcggaa gccggtgatc gttgcgacgg
     3001 agatgctgca gtccatggtg gactcaccgc ggcccacgcg cgccgaagcc agcgacgtcg
     3061 cgcacgccgt gttcacgggt gcggatgcgg tgatgctctc ggccgagagc gccacgggaa
     3121 agtttccgca cgcggccgtg cggatgatgg accggatcat tcgccaagca gaaggaagcc
     3181 gattctttct gccgaatcct tccgagcccg atcacaccac ggcagactcg atcgcgcatg
     3241 cctcggtggc gatcgcgcgc gaagtcggag ccaagctgat catcgctctg acggagaccg
     3301 gcgtgacggc ccggctcgtc tccaaggcgc gctccagtgt cccgatcctg gcgttttctt
     3361 cgggtgagcg taccttgcgc cagctggcgc tgttctgggg tgtcgcgccg cgcgcgctgg
     3421 ttgcgccgaa ggagaacttc gaggaccagg tgcgggacac gacgcagtac ttgctgcaaa
     3481 atggactggt caagtctggt gagcgctacg tgatggtgta cggcgcgcga gtcggcgtgc
     3541 gtggcgcgac gaactccgtg cgcgtggagc agctgccgtg agcgcggccc gcttgggtga
     3601 tgcgttgcgc ggagttaggg aggaagtagg ccctgagcgc ttcacctgaa cgggcaccga
     3661 agctcgagaa atacgagctg atcgaggaga tcggccatgg cggcatggct acggtgtatc
     3721 gcgcccgaga tcgtcgtctc gggcgcgacg tcgccgtgaa ggtgattcac catcatcttc
     3781 gtgaaaaccg cgaggtggcg cagcgcttcg agagcgaagc gcgcgcggtg gccaagctca
     3841 agcatccgaa catcgtcgag gtctacgacg tttcggatgc gcaagacgac gaacgctacc
     3901 tggtggtaga gctggtgcgt ggcaccacgc tgcgcaagct gctggccgcg gaaggccact
     3961 tgccgccgga agtggccgcg gcggtgggaa tcgagctcgc ggacgcgctg gagcacgcgc
     4021 acggcatggg cgtcatccac cgcgacatca agccggagaa cgtgctgatt cgggtgacgg
     4081 tgccggactc cgagcgcgaa cgttctcggg tgaagctgac cgacttcggc atcgcgaagc
     4141 tgctggacgc tcagggcgtc acctccaccg ggcaggtgct tggctcgcct gcgcacatgg
     4201 cgcccgagca gatcgagggc ggcgaagtga cggcgcgggc cgacgtgttc ggactcggtg
     4261 tgttgctcta cgagtgtatg gtcgggcgct tgccgttcga cggcaaaaac ccggctcaag
     4321 ttttgcgtcg ggtgctggac ggcgctttca ctccgcccga gcgagcgcgc ccgacggtcg
     4381 gcgcgactta ttcggccgtg ctcgcgcgag ccttggcgca cgagcccaag gaccgttacg
     4441 acagcactag cgctctggcg cacgatctgc gggcggaatt gttggccatg gggatcgagc
     4501 agcccgcact cgagttgcat cagtttttgg aagccccggc cgcgtaccgg gccgcggaag
     4561 ccgcgcgcgt cgtggagcgg ctcaggaaga acgcggaagg gctgcgcggt caaggcaaag
     4621 tcgtggaggc ggccgcctgc ttcaaccgcg cgctcgccta tcgccccaac gacggcgagt
     4681 tgttgcgcgc tctctcacgc ctgaatcgcg gcgcacgcac gcgacgagtc ttgctgcgcg
     4741 gagcttccac gatcgcgggc gtactcgggg ttgccggagc ggtggtcgcg gtggcacgca
     4801 tgcaaacgac gccgaagcgc gaggccaagg cggatgtgcc cacggcactc gttcggagcg
     4861 cggagccgat cccagtgagt tccttcgcgg tggtgtcttc gtcggcgcgc gctcaaccga
     4921 gcagcccaga gtcaccgccc gattccgcgc cgagcggcgc tccgacagcg cgcaagaacc
     4981 cccgcagcgg atcgtcgaaa cctgcccccg caagcagcgc ggaaggggcg ccccttgcgc
     5041 acgtccaaat cctcgtcgat ggtccacaga gcgcgatcgt gcgcgtggat ggcatcgaaa
     5101 cgtcttggtt tggcaggctg cgaagcatgc cacccggtcc acatacgttc gagttcgtgg
     5161 ccccggacga gacctgttgc gagcccggcc agacgctcaa agtcaatatc ccggtgccgg
     5221 tgaatgagaa cgacgtgacg accgtgcgcg ggcgaatcga gtttcgcccg gcaatgctcg
     5281 agctttccgg gccggccggg agtcttgcga gttgcggcgc tttgggagaa tttcccgtcc
     5341 ccggtcagcg tcaaatcccg atggcgggtg cggtccggcg ggtgcgttgt cagctcattc
     5401 ctcctcctgg aagcgcggag cctccaaaag agtttgacgt gacgctcagg gccggacgac
     5461 tctctaccaa tctcgggcag taggctgcga ttgggcccgg ggttgttcat gcggcccgtc
     5521 aattggttct tcgtcctgct gattacctat ggctcgccag cgatggctca atcgcgcggt
     5581 gcgacgagcg cgccgcgcgc cccagcgcaa gcctccgatg ccggattgaa tgacgaaacc
     5641 gagctcacgc gcgtggtcag cttgttcgag gcgggcaagt accggcagtg tgcagaggag
     5701 ttgaagggcc tgctttccga cgaagggacc cgccgtttgc gcgatcgcga cat
//
LOCUS       DASTKU010000968         8250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_189968, whole genome shotgun sequence.
ACCESSION   DASTKU010000968 DASTKU010000000
VERSION     DASTKU010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189968"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VFQ61_34360"
     CDS             complement(<1..739)
                     /locus_tag="VFQ61_34360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959643.1"
                     /translation="MTLDTAGRFVLERRVASRQACAALLMGLELLVKPAWPQTDRVND
                     SAPSRSEGGQQSRRQEQSGRVEQSTREEQSTLEEQSDPTGLRGVLGRYEVVAGCPDSS
                     WFLRQLGRRHTQNSREPGARLDVEVERYEAKFVGRLTDRAHPAQAVRQIEHARCTELL
                     QAMSLIAALMLEGRAELVAPSPEAHAEEVRSARQGDAMTVRGRAPYHFRHGPSVGLTL
                     LQGVAPDFRLGPRVGYGGWLTLEHGPVL"
     gene            833..2374
                     /locus_tag="VFQ61_34365"
     CDS             833..2374
                     /locus_tag="VFQ61_34365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9959644.1"
                     /translation="MANLLDAPGGSGPSGALPGATRLESAKELGKSPQANENPLAGAP
                     GKAASATPIFADDDAVEAPHSSQGEITVLVVDDEPSNVASLQKIFQRDGMRAFAAESA
                     KAALEIIRRHRVQVVLTDLMMPGTGGLELLRAIKELSPDTEVVLMTAYGTVETAVQAM
                     REGAYDFVEKPLKRMTIVKSVRKAAERHSLVAENRSLRQEIRALKHREIVGQSPALRR
                     VLDIATQAAPSSATVLILGESGTGKELIARFIHGKSARASGPFVAVNCAAIPESILEA
                     ELFGHERGAFTGATGRREGRFSRARGGTLFLDEIGELSPAVQVKILRVLQEGEFEPIG
                     GRTEKSDARIVAATNRDLLAEVEAGRFREDLYYRLNVIAVTAPPLRARREDIPLLVDH
                     FLGVYCKKNGRARLTVAPEVLSKLCDYPWPGNVRELENVIERAAVLCRADALGMDDLP
                     DSIARAASPAPSALTFPLGTPMAEVELRMIRETMKHTGGDKSLAAQLLGISTRTIYRK
                     LGEVE"
     gene            2511..4955
                     /locus_tag="VFQ61_34370"
     CDS             2511..4955
                     /locus_tag="VFQ61_34370"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9959645.1"
                     /translation="MTQTSAHIPAGSAKTGASPAPAAANATSAGGSTSTGPASSSPQS
                     GFTRPEIGSFQVKLRLYTEHMTVTSADGSEREQEVPVVALTFDYSGTEIHASDRRDRF
                     FVAAKGGVSLVERDRAGEARAQCLLESFGAVEVSCAEAYCPPLDSTADYLVHPDGNVH
                     AWCSFTAYAVPQLRALGWQVSIPLGYPYKVVSEDAQWYAGLMPGELGESDWFSLELGV
                     EINGSRVDLLPAMMELLDECSDTEALEALLRMPTRFRVLPVGEGQYLPVPPERLRSLV
                     RVILELYRGERLESGNLVIPSSRTTCVSDLDAAFASTGSELVWRGDRAAYEKGRSLSG
                     SPHAAPPVAHGLQAGLRPYQAEGVAWLQHLRAHDVGGILADDMGLGKTLQTIAHLVIE
                     SESGRMDVPSLIVVPKSLLGNWQRELTRFAPKLRLCIIAGSRRAEQMKRIKDSDVVIT
                     TYPILVRDIDEFSELPFHYLILDEAQAIKNPRSLSAGAVKALNARHRLCLSGTPVENH
                     LGELWALFDFLMPGFLGDKDRFRSKYRTPIERDGNESVLTSLRHVVSPFILRRMKEHV
                     ASELPPKTEIVRPVELEGDQRELYESIRVAAHGEVRNLIRKKGILGSTVLILDALMKL
                     RQVCCDPRLVGSHAARDVKTSAKHQLFFELLELQLSQGRRILVFSQFTRMLALLGKGL
                     EERGIPYVELTGDTADRQQRVDRFQAGEVDVFLISLKAGGTGLNLTRADTVIHYDPWW
                     NAAAQAQATDRAYRIGQTEPVFVYELIVSGSVEERMLRLKQKKAELSKGLLGNGGLSE
                     ALSEDDVDDLFSPLGD"
     gene            5191..6666
                     /locus_tag="VFQ61_34375"
     CDS             5191..6666
                     /locus_tag="VFQ61_34375"
                     /inference="COORDINATES: protein motif:HMM:NF013333.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959646.1"
                     /translation="MAAENLPGVSGMSLYVPPFRVRLSDWCAWTGNSWDKVKAVVGHE
                     FRVCGRHENVYTMAANAALRLILQNQLDPQRIGFLGLGTESSTDNAAGAVIVRGMLDR
                     ALESLGMPRLARNLEVPEFKHACLGGIYGLKNALRYVACDGRDRQAIVVSADVAEYER
                     GSSGEQTQGAGAVAMLVESKPRLFEVDLYHSGSASDYRGPDFRKPFARHFAEGYAAKT
                     TRPNDFPVFSGEYSTYSYIDETVQAVEDMLRRLEVSAGSYYRSVQSLFFHRPYHLMPV
                     RAMSFLYVRGLARGDHHQDELAELCAEAGVSPADVLHEAATAPDLYAHVLQGNITADP
                     YVKTSAVAAVLRKRAGFRELLAQKMSLGSDTVKSLGNLYSAALPAWIAAGLEEGAERK
                     LPLAETNMVAVGYGSGDAAEAIPISPVHGFEHAASRMHIRQALERAVDLTQEQYESLH
                     DHRELAGVDYAPQDEFVISRVGTVYESGFQDLGVEYYEYIR"
     gene            complement(6690..7556)
                     /locus_tag="VFQ61_34380"
     CDS             complement(6690..7556)
                     /locus_tag="VFQ61_34380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959647.1"
                     /translation="MSYAAKSSAIPALIVTIFLFRVELALAQAPSAPVQTTPAPGNSG
                     VPSSSVSQPNAPTQPSVATQPSVATQSSAARPQTNTQPSGMQSLPPRINLTPPPPEPV
                     EHRTYHMHDGFYARVSLGIGSLRARFDDDATTGYDLHASSGTFGAVGLDLMLGGSPTP
                     GVAVGGALLGVGTGGAETERGEFQTGDRGASLVMIGPFIDGFPKVNRGWHFGGMLGIA
                     SLKLQQADIDRIETTRGIGGAFWLGHDFWVADDWSIGPMLRFSGALTRDRDHDVSASA
                     FSALFLFTALYH"
     gene            complement(7741..>8250)
                     /locus_tag="VFQ61_34385"
     CDS             complement(7741..>8250)
                     /locus_tag="VFQ61_34385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959648.1"
                     /translation="HYENSGVPLMVLGSELGVRRDLRQGYLLAVSYGVLAARYLSDDS
                     FGAVFSFRKNPEFRSVANVPVHRAVVKGIAPIFGRSLSLASRITLESGRYDRLERRTE
                     VAQGRSSGVALWDLVLTGKVSDVGIDWAVGLYNAFDYRYSLPTSDEFPMRTVPQSGRS
                     FLLSLDVTL"
BASE COUNT         1577 a         2551 c         2611 g         1511 t
ORIGIN      
        1 cgagtacggg cccgtgctcg agcgtgagcc accccccgta acctacccgc ggtcccaagc
       61 ggaaatcggg agccacgcct tgcagcagcg tcaggccgac gctcggccca tgccgaaagt
      121 ggtacggagc gcgccctctc acagtcatgg catccccttg ccgcgcgctg cggacctcct
      181 ctgcatgggc ttccggcgag ggagcaacga gctcggctcg cccctccaac atcaaggccg
      241 caatcaaaga catcgcttgc agaagctcgg tgcagcgcgc gtgttcgatc tgacggaccg
      301 cctgggccgg gtgggcgcga tcggtgagtc gcccgacgaa cttggcctcg tatcgttcga
      361 cctcgacgtc tagtctcgct ccaggctccc gggagttctg cgtgtgccgc cgacccagtt
      421 gcctgagaaa ccaactcgag tccgggcatc ccgcgacgac ctcgtagcgc cccagcacac
      481 ctcgcaagcc ggtcgggtcg ctctgttctt ctagtgtgct ctgttcttcc cgcgtgctct
      541 gttcgactcg tccgctttgc tcctgccgtc tgctctgttg ccccccctcc gacctcgacg
      601 gagctgagtc gttcacccta tccgtttgtg gccaagccgg cttcaccagc agttccaagc
      661 ccatgagcag cgccgcgcag gcttgtcggg aagccacacg ccgttccaga acgaaacggc
      721 ccgctgtgtc aagcgtcaat acatggacgg attagcagaa cgtcttttca ttggtgacga
      781 tcagtcatga gccttgccaa gttgaccagg cgtctgccag aatgaccgac acatggccaa
      841 cctgctagat gcgccgggtg gatctggacc gagtggggcg ctgccgggag cgacccgact
      901 cgagtcagcg aaggagctgg gcaaaagccc gcaagccaac gaaaatcctc tcgccggagc
      961 gccgggtaag gcggcaagcg caacgcccat tttcgccgac gacgatgcag tcgaagcgcc
     1021 acactcgagc cagggtgaga tcacggtgct ggtggtcgac gacgagccgt cgaacgtcgc
     1081 gtccctccag aagatctttc aacgggatgg catgcgggcc ttcgcggccg agagcgccaa
     1141 agccgcactc gagatcatcc ggcgccaccg ggtgcaagtt gtcctgactg acctgatgat
     1201 gcccgggacg gggggattgg agttgttgcg ggcgatcaag gagctctcgc cggacacaga
     1261 ggtcgtcttg atgactgcgt acggaacggt cgagacggcc gtgcaggcca tgcgcgaggg
     1321 agcttacgac ttcgtggaga agccgttgaa gcgcatgacc atcgtcaaga gcgtgcgcaa
     1381 agccgcggag cgtcactcgt tggtggcgga gaaccgctct ttgcgacagg agattcgagc
     1441 cttgaagcac cgtgagatcg tcgggcagag ccccgcgctc cggcgcgtgt tggacatcgc
     1501 cacgcaggcc gccccttcga gcgccaccgt gctcatcctg ggtgagagcg gcacgggcaa
     1561 agagctgatt gcacgcttta tccatggcaa gagcgcccgc gcatccgggc ctttcgttgc
     1621 tgtcaattgc gcggcaatcc ccgaaagtat cttggaagcc gagttgttcg ggcatgagcg
     1681 gggcgcgttc accggcgcca ccgggcgacg cgaaggacgc ttttctcgag cgcgaggcgg
     1741 aactctcttc ttggacgaaa tcggagagct gtcccccgcc gttcaagtca agatcctgag
     1801 ggtcctacag gagggagagt tcgagccgat tggaggccgg accgagaaat ccgatgcccg
     1861 tatcgtggcg gccaccaacc gcgatctgct cgccgaggtg gaagcaggtc gctttcgaga
     1921 agatctctac taccgattga acgtgatcgc ggtcaccgcg ccgccgctgc gcgcgcgacg
     1981 tgaggacatt cccctgctcg tggatcactt cctcggcgtc tattgcaaga agaatggccg
     2041 agcccgcttg acggtcgccc ccgaggtgct gagcaagctg tgcgactacc cgtggcccgg
     2101 taacgtgcgc gagctcgaga acgtgatcga gcgcgccgcc gtcttgtgcc gcgccgacgc
     2161 gctcggcatg gatgacctgc cggattcgat agcccgcgcg gcgtcccccg cgccctcggc
     2221 gttgaccttc ccgctcggca cgccgatggc cgaggtagag ctgcgaatga tccgggaaac
     2281 catgaagcac actggcgggg acaagtcgct ggcagcccag ctcttgggca tctcgacacg
     2341 caccatttat cggaagctcg gtgaggtcga gtaacctcga tttgacgcca aaaagccgct
     2401 caccgcgggt tcgagtagga acaggtagtc aaaatcgagc caaatttctg agccttcggc
     2461 gctttatcgg attgtgagtt tcgaaacacg gcactaggct cggccgctcc ttgacccaga
     2521 ccagtgcgca tatccctgca ggctccgcca aaaccggcgc atcgcccgcg cccgcggccg
     2581 ccaacgcgac ctcggcaggc ggctcgacca gcacaggacc ggcatcgagc tctcctcaat
     2641 ctggcttcac gcggcccgag atcgggagct ttcaggtcaa gctgcggctt tacaccgaac
     2701 atatgacggt cacgtcggcg gacggcagcg aacgcgagca agaagtgccg gtcgtggcgc
     2761 tgaccttcga ctactcgggg accgagattc acgcgagcga tcggcgtgat cgcttcttcg
     2821 tggctgcgaa gggcggggtg tcattggtcg agcgcgaccg ggccggggaa gcgcgcgcac
     2881 agtgcttgct cgagagcttc ggtgcggtgg aggtatcgtg cgcagaggca tattgcccgc
     2941 cgctcgattc cacggcggac tacctggtgc accccgacgg caacgtgcac gcctggtgtt
     3001 ccttcaccgc atatgcggtc ccgcagctgc gcgcgctcgg atggcaagtc agcatcccgc
     3061 taggctatcc gtacaaggtc gtgtccgaag acgcgcagtg gtacgccggt ctgatgccgg
     3121 gtgaactcgg cgagagcgac tggttcagcc tggaactcgg ggtggaaatc aatgggagcc
     3181 gcgtcgacct tctaccggcg atgatggagc tgctcgacga gtgctccgac accgaagcgc
     3241 tcgaggcttt gttgcgaatg ccgacccggt ttcgggtgtt gcccgtcggc gaagggcaat
     3301 acttgcccgt acccccggag cggttgcgct cgttggtgcg cgtgattctc gagctctatc
     3361 gcggcgagcg attggagtcg ggcaacctcg tcattccttc gagccgcacg acctgcgtct
     3421 cggatctgga tgcggccttc gcttcgactg gcagcgagct ggtgtggcgt ggagatcgag
     3481 ccgcgtacga gaaaggtcgc tctctgtccg gctccccgca cgcggcgcct cccgttgccc
     3541 acgggctgca agcaggcttg aggccgtacc aagcggaggg cgtggcctgg ctccagcatc
     3601 tgcgcgcgca tgatgtcggc ggcatcttgg ccgacgacat gggtcttgga aaaaccctgc
     3661 aaaccatcgc ccacttggtg atcgagagcg agtcggggcg catggacgtg ccaagtctca
     3721 tcgtggtgcc gaagagcctg ctcggaaatt ggcagcgaga actgacccgc ttcgccccca
     3781 agctgcgctt gtgcatcatc gccggcagcc gacgcgcgga gcaaatgaag cgcatcaagg
     3841 attcggacgt ggtgatcacc acctatccga tcttggtgcg agacatcgac gagttctcgg
     3901 agctgccttt ccattacttg atattggacg aagcccaagc catcaaaaat ccgcgcagtc
     3961 tgagcgcagg cgcggtcaag gcgctcaacg cgcggcaccg actctgcttg agcggaactc
     4021 ccgtcgagaa tcacttgggt gagctttggg ctctattcga tttcttgatg cccggcttcc
     4081 tcggcgacaa ggatcgattc cggagcaagt accgcacgcc catagaacgc gatggcaacg
     4141 agagcgtgct cacgtcgctc cgacacgtgg tctctccttt catcctgcgt cggatgaagg
     4201 agcacgtagc tagcgagctc ccgcccaaga ccgagatcgt gcggccagtg gagctggagg
     4261 gagatcaacg cgagctgtac gagagcattc gcgtcgccgc ccatggcgag gtgcgcaacc
     4321 tcattcgcaa gaagggcatc ctcggttcga ccgtcttgat cctggacgca ctgatgaagc
     4381 tgcgccaagt ctgctgcgat ccgcgcctgg tcggcagtca cgccgcccgc gacgtgaaga
     4441 cttcggccaa acatcaattg ttcttcgagc tgctcgaact gcagctctct caagggcgcc
     4501 gtattctggt gttctcccag ttcacccgca tgctcgccct attgggcaag ggcttggaag
     4561 aacgcggaat tccctacgtc gagctcaccg gggacacggc cgatcgtcag caacgggtcg
     4621 atcgattcca agcgggtgaa gtggacgtgt ttttgatcag cctgaaggcg ggcggcacgg
     4681 gcttgaatct gacccgtgcg gatacggtga ttcactacga tccgtggtgg aacgccgcgg
     4741 cccaggccca ggccaccgac cgcgcctatc gcatcggtca gaccgagccg gtgttcgtct
     4801 atgagctgat cgtgtccggc agcgtcgagg agcgcatgct gcggctcaaa cagaagaagg
     4861 ccgagctgtc caaaggctta ctgggcaacg gcggcttgtc cgaggcgctc agcgaggatg
     4921 acgtggacga tcttttctcc ccgctggggg actaattccg ttctcctcgc aggttctacg
     4981 ccccggccct gctcgagaac cgaacggggc ttggctccga gagactagtt ctcgacagcc
     5041 ccccccgaca cccaacccag ggggcactga atgaagcgag cgtagcgaaa acaactcgcg
     5101 tcagtgggca tatcggtcag ggtcgacaca gaagaatcag ccggccactt gccatgcgcc
     5161 tgccgcggca ctaccaactc tgcggaaaat atggctgcgg aaaacctgcc tggcgtcagt
     5221 ggtatgagct tgtacgtccc ccctttccgg gtgcgcctct ccgactggtg cgcttggacg
     5281 gggaatagct gggacaaagt caaagccgtc gttgggcacg agttccgagt gtgtgggcgc
     5341 cacgagaacg tttacacgat ggcagccaat gcggcgctgc ggctcatcct gcagaatcag
     5401 ctcgaccccc agcgcatcgg ttttctcggg ctaggcaccg agtccagcac ggacaacgcc
     5461 gcgggggccg taatcgtgcg cgggatgttg gaccgcgcac tcgagagcct gggcatgccg
     5521 cggttggcgc gcaacctgga agtgccagag ttcaagcacg cgtgtttggg cggcatttat
     5581 ggactgaaaa acgcacttcg ctacgtggcg tgtgacggcc gggatcggca agccatcgta
     5641 gttagcgcag acgtcgcgga gtacgagcgc ggtagctccg gtgagcagac tcaaggggcg
     5701 ggcgcagtcg cgatgttggt ggagtccaag ccgcgcttgt tcgaagtgga tctgtatcat
     5761 tcgggcagtg cctccgacta tcggggaccg gatttccgca aacccttcgc gcggcatttc
     5821 gcggagggct acgcggcgaa gactacgcgg cccaacgatt tcccggtttt cagcggcgaa
     5881 tactcgacgt actcgtatat cgacgagacg gtgcaggcgg tcgaggatat gttgcggcgc
     5941 ctcgaagtct cggcgggctc gtactatcga agcgtacaaa gcctcttctt ccatcgcccg
     6001 tatcacctga tgccggtgcg cgcgatgtcg tttctgtacg tgcgtgggtt ggcgcgcgga
     6061 gatcatcacc aagacgagct cgcggagctg tgtgccgaag ccggcgtcag ccccgcagat
     6121 gtgctgcacg aagcggcaac cgcgccagat ctctatgccc acgtgctcca aggcaacatc
     6181 accgcggatc cgtacgtaaa aacgagcgcc gtggccgccg tgctacgcaa gcgggccggc
     6241 ttccgcgaat tgcttgccca gaagatgagc ctgggtagcg acacggtgaa gtccctaggc
     6301 aacttgtaca gcgctgcgct gccggcctgg atcgccgcgg ggctcgaaga aggcgcagaa
     6361 cgcaagctcc ccctcgcgga gacgaacatg gttgcggttg gctacggtag tggggatgcc
     6421 gctgaggcga tcccaatctc acccgttcac ggcttcgagc acgcagcaag ccgtatgcat
     6481 atccgccagg cgctggagcg cgcggtggac ttgacccaag aacaatacga gtccctgcac
     6541 gatcatcgcg agttggccgg cgtcgactat gctccccagg acgaattcgt gatcagccga
     6601 gtcggcactg tctacgagtc tggctttcag gatctgggcg tcgaatatta cgaatacatt
     6661 cgctgatcgg gtgcgggggg gctccgcacc tagtggtaga gcgctgtaaa taagaatagg
     6721 gccgagaacg cgctggcgct gacgtcgtga tcgcgatctc tcgtgagcgc gcccgagaag
     6781 cggagcatgg ggccaatcga ccagtcatcc gcgacccaga agtcgtggcc cagccagaat
     6841 gcgcctccga tcccacgcgt ggtttcgatg cggtcgatgt cggcttgctg gagcttcaaa
     6901 ctggcgatcc ccaacatgcc gccgaagtgc cagccgcgat tgacctttgg aaacccgtcg
     6961 atgaaaggtc cgatcatgac cagactggcg ccgcgatctc cagtttgaaa ctcgcctcgc
     7021 tccgtctcgg cgccgcccgt acctactccc aacaaggctc cgcccacggc gacgccggga
     7081 gttggagacc ctcctaacat caaatcgaga cccaccgcgc cgaatgtgcc gctggatgca
     7141 tgcagatcgt acccggtggt ggcatcgtca tcgaagcgcg ctcgcaacga gccgattccc
     7201 aaactcacgc gtgcgtaaaa gccatcgtgc atatgatatg tgcgatgctc gacgggctct
     7261 gggggtggtg gcgtcaagtt gatgcggggc ggtagggatt gcatcccgct aggttgagtg
     7321 ttcgtctgcg ggcgcgctgc gctggattgt gtcgctacgc tgggttgtgt cgctacgctg
     7381 ggctgggttg gcgcgttcgg ttgtgagacg ctcgaacttg gcactccaga attaccagga
     7441 gccggcgtgg tctggaccgg agctgacggc gcctgcgcca gtgcgagctc aacgcgaaac
     7501 aagaaaatcg taacgatcag tgccgggatg gccgaagact tagctgcgta agacatgatg
     7561 cacctgtgaa tcagagttca cgaagtcact tccagggcgc gtgcagagtt cggagagctt
     7621 aagcgagttg atgaggctga gccagtcgcc ctggggacga ccggctgagc ctggttttgt
     7681 gacggaacgg gaccaccctt cattcagccg acgctcacct ttttgtcagg tggcattcgg
     7741 ttacagggtg acatccaaag aaagcagaaa cgagcgcccg ctctgtggca cggttcgcat
     7801 cggaaactcg tcactagtcg gcaatgagta ccgatagtcg aacgcgttgt acaagcccac
     7861 ggcccagtct atccccacgt cgctgacctt cccggtcaac accagatccc aaagcgccac
     7921 gccgctcgag cgcccctgag ctacttcagt gcgccgctcg aggcgatcgt accgcccact
     7981 ttccagagtg atgcgtgaag cgaggctcag actgcgcccg aagattggcg cgattccctt
     8041 taccacggcg cgatgaacgg ggacgttcgc cacactgcga aactcagggt tcttacggaa
     8101 agagaacacg gccccgaagc tgtcatccga aagatagcgc gccgccagca caccatacga
     8161 gaccgcgagc aggtaacctt gccggagatc gcgccgcact ccgagctcgc tgcccaacac
     8221 catcaggggc acaccgctgt tttcgtagtg
//
LOCUS       DASTKU010000969        13706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1307552, whole genome shotgun sequence.
ACCESSION   DASTKU010000969 DASTKU010000000
VERSION     DASTKU010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13706
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307552"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFQ61_34390"
     CDS             complement(<1..551)
                     /locus_tag="VFQ61_34390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959649.1"
                     /translation="MPLRKTWLSSGVLCLCLVPALVGWPARALAQAGDKAAAEVLFNE
                     GRQQMQEGKLDSACDKLAESQRLDPGAGTLLNLAECRERQGRTATAWATWLEAAAAAH
                     TAGQSEREAHARKRAEGLKPRLVTLTLEVPEASRISGLEVSRDGVALGAAMWGVAVPV
                     DPGAHTLTAKASGREPWTQTVSVV"
     gene            complement(671..1774)
                     /locus_tag="VFQ61_34395"
     CDS             complement(671..1774)
                     /locus_tag="VFQ61_34395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET9959650.1"
                     /translation="MHAIVLAGGSGTRFWPASRRVNPKQLLPLGPGASPGDAGGRPRS
                     LIEQTVHRLAPWCSRERVWVATGAHLLDATRALLPDLPASAFLGEPVAKNTAPCIAWA
                     TAVIEQADPGAYVIVIPSDQYVGMPAAFEAAVSQALDSAATAITTIGIKPTRPETGFG
                     YIELGDNVTNGVYRVARFVEKPDLARAVAYMEGGRHLWNSGMFFYRAGDLLSAIAEHA
                     PEIHAGLSRLKADSALTAGADPALLQAVFAAFPSISIDYAVMERMARLHVVPGDFGWS
                     DLGSWESAWELAAHDENGNALSDHAIYVDARGNLVVDLSQQASKRVTALLGVEGLCVV
                     NTDDALLVIPRERAQDVRYIVDALKRGNRVDLT"
     gene            complement(2098..3474)
                     /locus_tag="VFQ61_34400"
     CDS             complement(2098..3474)
                     /locus_tag="VFQ61_34400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959651.1"
                     /translation="MKTPLSGSDPVDCGWDTELERALVPLESLPRPKNQPRPANQPKS
                     FETPPKSFEARAQSFEAETESLEAATESFEVETNQPADHELDQELTPNTLESRGNIVV
                     AVSVPPPPTVTREEETRTVVISRPSDEPTLVRSQNGSSKGVNSQRPSATASSGPEEVA
                     LSPFAAPLISIDEALSAWTEPAADAARSDQPGLSARAPLEEGAPELPVNPHPHPRRHR
                     HMIESGTLRPNELCVPVQRATPTRSRIGLLGMFAIAAPLSFMLGAGLARYQSYATHTH
                     NLIVARVSELTGSSAAASAARAEGSSKASVAVAVQSANAPSSPPAPNSEAPSVPVAET
                     ASPATAEATDNTEPRVIETRSVRVRVIPEQAELWEQGHRLGVGRIIVDVPSGESRELT
                     ASLRGHRARMVVVDGNHEKVVIILDPIEKAPSSAIKTAAARSSARAPVEPPPATDVDP
                     VLPHMSLR"
     gene            3890..4675
                     /locus_tag="VFQ61_34405"
     CDS             3890..4675
                     /locus_tag="VFQ61_34405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959652.1"
                     /translation="MIKRTALVALGLLLCSGTAWADEDDADSSSKEASEESSNPESDS
                     SSEEPSEPVLRRLGNAGDVVISAERIAGIARTHRHVSVPNAPDLNEGVSRTNLLLNAN
                     GDALGYSAPRLALDYFVADAWSLGGSLGYSSDGHNKSSRYSQLTAHARVGYAWMFANW
                     IGIWPRAGLTFVQAKRGGPNLTGATQLSSTALLSAGGATVSLLAASADLALVLMPAKR
                     TMITLGPTFDLSFLGKFNPDGPTGSLDMTAREVGLQMGVAITF"
     gene            4878..5990
                     /locus_tag="VFQ61_34410"
     CDS             4878..5990
                     /locus_tag="VFQ61_34410"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET9959653.1"
                     /translation="MSTRASERSRTSILRNWLFSSIALAAASLGSSAGCSKSKAAPNP
                     PPEAKAELKASSPASGQSPTPSAGAPLTVAYSDWPGWVAWDIGVAKGWFKEAGVDVSF
                     KWFEYAPSMDAFSAGKVDAVCVTNGDALVTSANGAKSVAILLNDFSDGNDMVIAKPGI
                     KSISDLKGKKVGVEVGFVDHLLLMYALKSKNLGEKDVTLVNMPTDQAAQTLKAGDVSA
                     VAAWQPNSGAALSLVPGSTPIFTSADVKGLIYDELVVNPKSLAEHRREWLAVVKVWDR
                     IAQFVNNGENLNEAAKIMSARVGLDPEKYKALMKGTRFLDLKASLKHWEKGDGLESVY
                     GSSRIVDEFNVKNKVYKAASKIEEHLDPSLTAEATR"
     gene            5987..7171
                     /locus_tag="VFQ61_34415"
     CDS             5987..7171
                     /locus_tag="VFQ61_34415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9959654.1"
                     /translation="MKRRRGEPTAKRRVALWLGSFALPLLIWSAISYVPFVWHPLVLV
                     QDAGSSGVPGPYDFVSAGQRVERTVFELRNAELQRAGKAPMRGERVNPIFLPAPHEVA
                     TAFVTAFRTPPERNGDVWLHESLAHSVRVIFLGFIYSVLWGLPLGILCGTFRPIAALT
                     EPFVDFIRYMPAPAFGALCVAIFGLGDEPKISIIFIGTFFQLVLVVANTTRSVEKGLF
                     EAAQTLGASRVQLVTHVVIPAALPALFRDLRILLGWAWTYLVVAELIGEKSGISAFIY
                     QQQRYMHYDNVYAAILMIGFLGLFTDQVLSRLGNLLFPWESGNHRLFRWFARLSRASA
                     NRKLGPKAPPPETQAARLNALASPSRPPTPTQGSELGITRSGLRPLPPLGEPQGALSR
                     LR"
     gene            7214..8122
                     /locus_tag="VFQ61_34420"
     CDS             7214..8122
                     /locus_tag="VFQ61_34420"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9959655.1"
                     /translation="MSSGDERFKGDQLSLPSYLHHTDEVKARLSELSRSPTVLRVQDL
                     GREYESANGPLQALRGVEFSVREGEFMSVIGPSGCGKSTLIRIIAGLDHPSSGAVVVD
                     TEPVRGPARDRGMVFQNYTLFPWLSVKRNVMFGLRMRGLRRREAEERACHWLELVGLI
                     DFRDHYPAQLSGGMKQRVAIARALCNEPRILLMDEPFGALDAQTRCQMQAHLLRIWEQ
                     AKVTILFITHDLDEAIHLSDRIVVLGRNPGRVLEVIEVPVPRPRSSSQLLTGHFLSAK
                     RRLEELIHERETDSKPNAGKLSNVAA"
     gene            8239..8967
                     /locus_tag="VFQ61_34425"
     CDS             8239..8967
                     /locus_tag="VFQ61_34425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP1"
                     /protein_id="HET9959656.1"
                     /translation="MQDTSAPRVIVEEALRGGQMWSRVLRRGQSLRLTDLEGGAAVAA
                     LFYNADDKLERYNMPDTLKAQHTARLTRGHVLYSDMGRVLCAITEDSLGHHDTITGHG
                     TRTHQQAKYGEGSYQKLRNDFYRNSRDNFLVELGKHELGLRDIVANVNWFVKVAVDDF
                     GRLSFVPDHSKPGSYVELRAELNTLVVLSNTPHPMDPRRNYAPGPVALTVYDTVADSR
                     RRDVCRLSCPENARGFALTDAYFE"
     gene            8973..9644
                     /locus_tag="VFQ61_34430"
     CDS             8973..9644
                     /locus_tag="VFQ61_34430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP2"
                     /protein_id="HET9959657.1"
                     /translation="MTLSNSSATGFPILASARDPADAIFDQVVEAGRGFTHTLGRGQI
                     LRIVDLEGNQAVDTLFYSLADPLDRYSAVDTIREQGNVYLGAGTRLLSVHGRHLLSIV
                     ADTCGRHDTLGGACAAESNQVRYALEKKYMHNCRDNFLLTGCQCGIGKRDLTANINFF
                     MNVPLTPGGKLTFEDGISAPGRYVELEAQVDVRILISNCPQLNNPCNAYNPTPIQVLI
                     WNAPS"
     gene            9739..13485
                     /locus_tag="VFQ61_34435"
     CDS             9739..13485
                     /locus_tag="VFQ61_34435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase/urea amidolyase family protein"
                     /protein_id="HET9959658.1"
                     /translation="MFESVLIANRGEIACRISRTLRALGARVIAVYSDADRKSRHVRE
                     ADLAIRLGPAPARESYLNAERVLMAARESGAQAIHPGYGFLSENADFAQAVEAAGLAF
                     IGPTPDQIRAFGIKHRARELAERAGVPLLPGSGALDDLSHAVREAERVGFPVMLKSSA
                     GGGGIGMQLVREPAALPKAYESVRFAGRSNFGNAALFLERFVEHARHIEVQVFGDGAG
                     AVVTLGERDCSAQRRNQKVIEEAPAPNLPRKVREALHGAARELLSSVSYRSAGTVEFI
                     YDAECEQFYFLEVNTRLQVEHGVTELVRGIDLVEWMVRTAAGDAPDLASLPRESGHAI
                     EVRLYAEDPAREFRPSSGLLTQLEFPQNARVDTFLEVGDEVASDYDPMLAKIMAHGAD
                     RDGALSALESSLAETRIGGIRTNLDYLKALLTSPTVRQGRVLTRTLPHFAYADGGVEI
                     IAPGTFSILVDYPGRTGYWSVGVPPSGPMDDWSARIANGIVGNPEDCAVLEMTHTGAT
                     LRFSADQCIALTGARMRATLDGKSVSYGRPLEVRAGQVLQLGSLEGGGQRSYLAIRGG
                     FDVPSYLGSRTTFTLGGFGGHAGRCLRKGDVLFAQARTDLRPVREAPIEFLPAFGKTW
                     EIRVMYGPHGAPEFFTRRDIDTLFSAEYEVHYNSSRTGVRLLGPKPEWARPDGGEAGL
                     HPSNIHDVAYAIGAIDFTGDMPILLGPDGPSLGGFVCPATIPQAELWKMGQLHPGDRV
                     RFIPIALETALTLEHQQRAALTHFPSLTPLPAAAPNSTSPTQAPAPTSEAQTPAPTSQ
                     ARAPDPSQAGLGPRAQRGAPWEGGPAAGGLEQTLQTIQQTLPITIRPAGDRYLLVEYG
                     PLVLDLRLRFRVHALMTWLERASLPGVLDLTPGVRSLQIHYDPARLPAAQLRSLIERA
                     EDELGDASDVAVPTRTVWLPLSWDDPETRRAIEKYTQLVRADAPWCPSNIEFIRRING
                     LADERAVYDTVFNASYLVLGLGDVYLGAPVATPLDPRHRLVTTKYNPARTWTPENAVG
                     IGGAYMCIYGMEGPGGYQFVGRTLQVYNRFRTTPEFAPGTPWLLRFFDQIRFYPVSAK
                     ELLELRESFLHGAHSIRIEPGTFRLSEYQTFLEDNQRSIALFKAQQQRAFEAERARWA
                     EQALAEPSEEASISAAVPANLDALPEGVVSIDSHVTGSIWKLAVERGARVKKGDVLVV
                     LESMKMEISVEASHDGTVTELSVGEGRAVSAGQRLMLIEQAEAS"
     gene            13482..>13706
                     /locus_tag="VFQ61_34440"
     CDS             13482..>13706
                     /locus_tag="VFQ61_34440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959659.1"
                     /translation="MNDLSPRHLARAYAARERDPQQVLNEVYDRIARNGSSPVWTHVR
                     PYESLLRDLEFAEANIRPDARGPLFGVPFAV"
BASE COUNT         2533 a         4347 c         4315 g         2511 t
ORIGIN      
        1 accacactga cggtctgcgt ccagggctcc cgccccgaag ccttggcggt cagggtatga
       61 gcaccgggat ctaccggcac ggctacgccc cacatggcgg ccccgagcgc gacgccgtct
      121 cgcgacacct cgagccctga aatgcggctc gcttccggca cctccaaagt caacgtgacg
      181 aggcgtggct tgagtccctc ggcgcgcttg cgagcgtgcg cctcgcgttc cgattgaccc
      241 gccgtgtgcg ccgccgcggc ggcttcgagc cacgttgccc aggccgtcgc agtgcggcct
      301 tgccgttcgc gacactcggc caggttcagc aaggtgcccg caccgggatc caagcgttgg
      361 ctctcggcca acttgtcgca ggccgagtcc agcttgccct cttgcatctg ctgcctacct
      421 tcgttgaaca gcacctcggc ggccgcctta tcccctgctt gcgccaacgc gcgagcaggc
      481 cagccgacga gagccggaac caagcacagg cacaacacac cagaagaaag ccaagttttg
      541 cgcagaggca tgtccacctt agcccaagtt tctcgggttc cgctcgcaaa aagctctggc
      601 tgcggcaccg acccgacgca ccgaccgagc cgcgggctcc gcgtgcgcgt gcccgcctcc
      661 ccgtgagtgc tcaggtcaag tcgacgcgat taccacgttt cagggcgtcg acgatgtaac
      721 gcacatcctg ggcgcgctca cgcgggatca cgagcagcgc atcatccgtg ttcacgacgc
      781 acaacccctc cactcccagc agcgccgtca cgcgcttcga ggcctgttga cttagatcca
      841 ccaccaggtt gcctcgtgca tcgacgtata tggcgtgatc cgaaagcgca ttgccattct
      901 cgtcgtgtgc ggcaagctcc caggcgctct cccaggagcc caggtcactc cagccgaagt
      961 cccccggcac gacgtgcagg cgcgccatcc gctccatcac cgcataatcg atgctgatcg
     1021 aaggaaacgc ggcgaatacg gcctgaagca gcgcgggatc cgcccctgcg gtgagcgcgg
     1081 aatcggcctt gaggcgcgac aatccggcgt gaatctcggg cgcgtgctcc gcgatcgcgc
     1141 tcagcagatc cccggcgcgg tagaagaaca tcccgctatt ccagaggtgg cgcccacctt
     1201 ccatataagc aacggcgcgc gccaaatcgg gcttctcgac gaagcgcgct acgcgataca
     1261 cgccgttggt gacgttgtcc ccgagctcga tatagccaaa gcccgtttcg ggtcgcgtgg
     1321 gtttgattcc gatcgtggtg atggccgtag cggcggaatc cagggcttgt gataccgcag
     1381 cttcgaaggc ggcgggcata ccgacgtatt ggtcactcgg gatcacgatc acgtacgcgc
     1441 ccggatcggc ttgctcgatg acggccgtcg cccaagcgat gcatggggcc gtattcttcg
     1501 ctaccggttc acccagaaac gccgacgcgg gcaggtccgg caagagggcg cgagtagcgt
     1561 ccaacaagtg cgcaccagtc gcaacccaga ctcgctcacg ggaacaccac ggagccaacc
     1621 tgtgcaccgt ctgttcgatc aacgagcgag ggcgcccgcc agcgtcgccg ggagaagcac
     1681 cgggaccgag cggcaagagc tgctttggat tgacacgccg agaggccggc cagaatcggg
     1741 tcccagagcc tcccgctaaa acgatggcat gcaccgaacg ggaggagccc ttcgaagttg
     1801 cattggatgg atgggatgaa gttcgatcgg tcgcgctcat gtcgtagtgg gcgcggagag
     1861 tagcccagcg ccgcgctgac gacgtagtct ttctctcgac ccttctttgg acggcccctt
     1921 cgccgccctt tcagcaccgt ggaacggccg gggatgaggc gccggcgttg tagtcgcgcg
     1981 gcaagcgcgg ccgtgagacg aaagagacgg aaagcgcggc cggggcgtgc acccctgccg
     2041 agcagcgcgc acttttctat gaggccgcct gcttacggga agccgatgcg gcgccgctca
     2101 gcgcagggac atgtgcggca gcaccgggtc gacgtcggtt gcgggcggag gctctacggg
     2161 ggcacgcgcg gagctgcgag cggccgcggt cttgattgcg ctgctcggag ccttctcgat
     2221 cggatccaag atgatcacga ccttctcgtg attgccgtcg acaacgacca tgcgtgcgcg
     2281 gtgaccacgg agcgaggccg tgagctctcg cgactcgccc gagggaacgt cgacgatgat
     2341 gcgaccgaca ccaagccgat gcccctgctc ccagagttcg gcttgttccg ggatgacgcg
     2401 tactcgcacc gagcgcgtct cgatgacgcg aggctcggtg ttgtcggtcg cctccgcggt
     2461 tgcaggtgat gcagtttcgg cgacgggcac actcggagct tcggagtttg gagcgggagg
     2521 gctggaaggc gcattcgcgg actgtaccgc cacggctacg ctcgctttgg agctcccctc
     2581 cgcgcgcgct gccgatgccg ctgcggaaga gcccgtgagc tcgctcaccc gcgcaacgat
     2641 gagattgtga gtgtgcgtcg cataggactg atatcgcgcg agccctgccc cgagcataaa
     2701 ggacagaggc gccgcgatcg cgaacatccc gagcaacccg atgcgcgagc gtgtcggggt
     2761 cgcgcgttga acgggcacgc agagctcgtt gggccgcagc gtaccagact caatcatgtg
     2821 ccgatgacgg cgtgggtgcg ggtgtggatt gacggggagt tcgggtgcac cctcctccaa
     2881 aggcgcccgc gcggataacc ccggctgatc gctgcgcgcc gcgtccgcgg cgggctccgt
     2941 ccacgcactc agggcctcat cgatcgagat caggggcgca gcgaaggggc tcaaggccac
     3001 ttcctccggg ccgctcgacg cggtcgcgct cggtcgctgc gagttcacgc ctttggagga
     3061 tccattctgc gaacgcacga gagtcggctc gtccgaaggt cgcgagatga ctacggtgcg
     3121 ggtctcctct tcacgagtga cggtgggggg cggcggaacg gatactgcta cgacaatatt
     3181 cccccgcgac tcgagcgtat tgggggtcag ctcctgatcg agctcatggt ccgcgggctg
     3241 gttcgtctct acttcgaacg actccgttgc cgcctcgagg gactccgttt ctgcctcgaa
     3301 ggactgggca cgcgcctcga acgacttcgg gggggtttcg aaagacttcg gctggtttgc
     3361 gggcctcggc tggtttttgg gtctcggtag cgattccaga ggcacgagcg cgcgttcgag
     3421 ttcggtgtcc cacccacagt ccaccgggtc ggagccagaa aggggggtct tcacgcccat
     3481 atctattcag taacccgaag agggtgattc gggtcacttc gatagatcga accctgaaag
     3541 tatcgctgtt ttgccttacc aatttttgat cgcgttacca tcacgagccc gagcgaaccc
     3601 tgcctgaagc cggaggtgag tgagcaggct gtttcgcgct ggatcagcgt gacatcacgc
     3661 attcgcggga aggacaccgg ctgcgcaact cgtgcaatcg accgagccgt tcacggcatg
     3721 acgctcggcc gactgatgtc gcggcactac accaacgact gccccgaatg ctcaagtttt
     3781 ctgcaaaaaa atctgacgac agaacagcgc actcgctaag ccgcgacgtt tcgcgagggc
     3841 ttccgaagaa cacttggaag cggctacact gcttggttgg agccaaccca tgatcaaacg
     3901 aactgccctc gtcgcacttg gtctgttgct atgtagcggg acggcttggg ccgacgagga
     3961 cgacgctgat tcttcaagca aagaggcctc cgaggagtcg agcaacccgg agagtgactc
     4021 gagctcggag gagccgagtg aacccgttct tcgtcgcctc ggcaacgcgg gggatgtagt
     4081 gatttcggcg gagcgcatcg ccggaatcgc acgcacccat cggcacgtgt cggtgcccaa
     4141 tgctccggac ttgaacgagg gcgtttcgcg cacgaacctc ctcctcaatg cgaacgggga
     4201 cgccctgggt tactcggccc ctcgccttgc gctcgactac ttcgtggctg acgcgtggtc
     4261 gctcggaggc tcgctcggtt actcgtcgga tgggcacaac aagagtagcc gatattctca
     4321 gctcaccgcg cacgcccgcg tgggctacgc ctggatgttc gccaactgga ttggcatctg
     4381 gccgcgcgcg gggttgacct tcgtacaagc caagcgcgga ggcccgaact tgacgggagc
     4441 gacgcagctg agttctacgg cgctgctttc cgcaggggga gccacggtga gtctgctcgc
     4501 cgcaagcgcc gacttggcac tagtactgat gcccgccaag cgcacgatga ttactctggg
     4561 ccccaccttc gatctcagtt tcttgggcaa gttcaatccc gatggcccaa ccggttctct
     4621 cgatatgact gctcgcgaag tcggcctgca aatgggcgtg gcgattacgt tctgagctcg
     4681 agccgcgagg cggggagtcc attccagggt acgccgccga ttcggcgcga tccaggctcc
     4741 ccgcagccat ctcgtttcag tgtacgcgag gggcggctca cacttcgttt cgaaacgaac
     4801 gggacacccg ctcggaacac gcttagcgcc agggtcgcgc catgccctgc atcggcccca
     4861 ccccaggtgc tcggttcgtg agcactcgtg cgtctgagcg ttctcgcacg tctattctcc
     4921 gaaattggct attttccagc atcgcgctcg cggcggcgag ccttggatcg agcgctggct
     4981 gcagcaagtc caaggccgca cccaatccgc caccggaagc caaagccgaa ctgaaagcga
     5041 gctcgcccgc gagcggacag agcccaacgc cctccgccgg cgcgccattg acggtggctt
     5101 acagtgactg gccgggctgg gttgcttggg acatcggtgt agcgaaaggc tggttcaaag
     5161 aagccggcgt ggacgtgtct ttcaagtggt tcgaatacgc gccatcgatg gatgcgttct
     5221 ccgcgggaaa ggtcgacgcc gtgtgcgtca cgaacgggga cgcgctggtg acttccgcca
     5281 atggagccaa gagcgtggct attctgctga acgacttcag cgatggcaac gacatggtga
     5341 ttgccaaacc gggcatcaag agcatcagcg acctcaaagg caagaaggtg ggagtcgagg
     5401 tcgggttcgt cgatcatctg ctgttgatgt atgcactcaa gtccaagaat ctcggcgaga
     5461 aggacgtcac gctcgtgaac atgcccaccg atcaggcggc gcaaaccctg aaggcggggg
     5521 acgtctccgc ggtggcggct tggcaaccga acagcggggc cgcgctctcg ttggtgcctg
     5581 gctccacgcc gatcttcacg agcgcggacg taaagggcct gatttacgac gagctagtcg
     5641 tcaatcccaa gagcctcgcc gagcaccgcc gtgaatggct cgcagtagtg aaggtttggg
     5701 atcgcatcgc gcagttcgtg aacaacggcg agaatctaaa cgaagccgcc aagatcatga
     5761 gtgcgcgcgt gggcttggac ccagaaaagt acaaagcgct gatgaaaggg actcgctttc
     5821 tggatctcaa ggccagcctg aaacactggg agaagggcga tggcctggag agcgtctacg
     5881 gttctagccg catcgtggac gagttcaacg tcaagaacaa ggtctacaaa gctgcgtcta
     5941 agatcgaaga acacttggat ccgagcctga cggccgaggc gacgcggtga agcgacgtcg
     6001 gggcgagccc acggccaagc gtcgcgtcgc gctttggttg ggatcgttcg cactgccact
     6061 cttgatctgg agtgcaatta gttacgtgcc gttcgtttgg catccgctcg tgctggtgca
     6121 ggacgcgggc agtagcggag ttcccggacc gtacgatttc gtgtcggcgg gacagcgggt
     6181 ggaaagaacg gtattcgagc tcaggaacgc cgaactccag cgcgcgggca aggcgccgat
     6241 gcgaggcgaa cgggtcaatc ccatctttct ccccgcgcct cacgaggtag ctaccgcgtt
     6301 cgtcacggca ttccgcacgc ccccggaaag gaatggggac gtgtggttgc acgagagcct
     6361 agcccacagc gtgcgcgtga tcttcctggg cttcatctac tcggtgttgt gggggctccc
     6421 gctcgggatc ttgtgcggaa cctttcgccc cattgcggcg ctgaccgagc cgttcgtcga
     6481 cttcattcgc tacatgcccg cacccgcgtt cggcgccttg tgcgttgcca tattcgggct
     6541 cggagacgag ccgaaaatca gcatcatctt cattggcacc tttttccagc tcgtgctcgt
     6601 ggtggccaac accactcggt cggtggagaa agggctgttc gaagcggccc aaacgctggg
     6661 cgcgagccgg gttcagctcg tcacccatgt ggtgatcccg gccgcgctgc ccgcgttgtt
     6721 ccgggatctg cggattctgc tgggctgggc ttggacgtat ttggtggttg cggaactaat
     6781 cggcgagaag tctggaatca gcgccttcat ttatcagcag cagcgctaca tgcactatga
     6841 caatgtgtat gccgccattc tcatgattgg ctttttgggc ctgttcaccg atcaagtgct
     6901 ttcgcggctg ggcaacctcc tgttcccgtg ggaaagcggc aatcatcggt tgttccgctg
     6961 gttcgcgcgg ctgagccgcg cgagcgcaaa ccgaaagctt gggcccaaag cccctcctcc
     7021 cgaaacgcag gcggcgcggc tcaacgcgtt agcgtccccg agccgtccgc ccacgccaac
     7081 ccaggggagt gaactgggaa tcactcgaag cgggctccgt cctctgccgc cgctcggcga
     7141 gccgcaggga gccttgagtc gattacggtg accacgctgc gagctagctg tatccgagtc
     7201 aggaggagcc ttcatgtcga gcggtgatga acggtttaaa ggcgatcaac tcagcttacc
     7261 aagctacttg caccacacgg acgaggtaaa ggcacggctg agcgagctga gccgcagtcc
     7321 cacggtgctg cgcgtgcagg atctcggccg ggagtacgag tccgccaacg gccctctgca
     7381 agcgttgaga ggggtcgagt tctcggttcg tgaaggcgag ttcatgagcg tgattggccc
     7441 ctctggctgc gggaaatcca cgctgattcg tatcattgcc gggctggacc atccgagctc
     7501 cggggcggtg gtggtcgaca cggagcccgt gcgaggtccg gcgcgcgatc gaggcatggt
     7561 atttcagaac tacacgctct ttccgtggct gagcgtcaag cgcaacgtga tgttcggcct
     7621 ccgcatgcga ggccttcgtc ggcgcgaggc cgaagagcgc gcctgccact ggctcgagtt
     7681 ggtgggcttg atcgattttc gagaccacta tcccgcccag ctatcgggtg gcatgaagca
     7741 gcgcgtagcg attgcacggg cgctctgcaa cgaaccccgc atcctgctga tggacgagcc
     7801 gttcggggcg ttggatgccc agacccgttg ccaaatgcaa gctcacctgc tgcgcatctg
     7861 ggaacaagcc aaggtcacga tcttgttcat cactcacgac ctcgacgaag ccattcatct
     7921 ttccgatcgg atcgtggtct tgggccgcaa tccaggtcgt gtgctcgagg tcatcgaggt
     7981 gcccgtgccg cgcccgcgca gctcgtcaca gctcctaacc ggacacttcc tgtcggcaaa
     8041 gcgtcgcctc gaggaactga tccacgagcg cgaaaccgac tcgaaaccaa acgcagggaa
     8101 actgtcgaac gtggctgcat gagccgagcg ccgtcgccag gccgctctgc tcgattggat
     8161 gcctgctgat tccaagtagc ccagcccgag aggctttgag ttcgcgatct ctcgttccga
     8221 agaggcgaag aggcaaacat gcaagatacg agtgccccgc gggtcatcgt cgaggaagct
     8281 ctgcgaggcg ggcaaatgtg gtcccgcgtt ctgcgtcgtg gtcagagctt acgtctgacc
     8341 gacttggaag gaggggccgc ggtagccgcg ctcttctaca acgcagatga caagctggaa
     8401 cggtacaaca tgccggacac gctgaaggcg cagcacacgg cgcgcctcac gcgaggccat
     8461 gttctgtact cggacatggg ccgcgtgctg tgcgcgatta cggaagactc gctcggtcat
     8521 cacgatacga tcaccggtca cggcactcgg acccaccaac aggccaagta cggtgaaggc
     8581 tcctatcaga agctgcgcaa tgacttctat cgcaacagcc gggacaactt cctagtggag
     8641 ctcggaaagc acgagcttgg gctgcgtgac atcgtcgcca acgtcaactg gttcgtgaag
     8701 gtggcggtgg acgacttcgg gcgtttgagc ttcgtgcctg accactccaa gccgggaagt
     8761 tacgtagaac tgcgagcaga gctgaatact ctggtggtgc tctccaacac tccccatccg
     8821 atggacccgc gtcgcaacta cgcgcccggg cccgtggcgc tgacggtcta cgatacagtg
     8881 gctgactcac gccgacgcga cgtttgccgt ctgtcttgcc ccgagaatgc gcgaggtttc
     8941 gcgctcaccg acgcctactt cgagtagcag ccatgactct ttcgaactcc tccgcaacgg
     9001 gtttcccgat tctagcgtcc gcgcgcgatc ctgcagacgc gatcttcgat caggtagtgg
     9061 aggcggggcg tggtttcacg cataccttgg ggcgtggcca gattctgcgc atcgtcgact
     9121 tggagggcaa ccaggcggta gacacgctct tctacagctt ggcggaccca ctcgatcgct
     9181 atagcgccgt ggacacgatc cgcgagcaag gcaacgtgta cctcggcgcg ggcacgcgac
     9241 tgctctccgt tcacggtcgt cacctgttga gcatcgtggc cgatacgtgc gggcgccacg
     9301 acaccttggg cggagcctgc gcggccgaat ccaatcaggt gcgatatgcg ctcgagaaga
     9361 agtacatgca taactgccga gacaacttct tgctcaccgg ctgtcagtgt ggaattggca
     9421 aacgcgacct caccgcgaac atcaacttct tcatgaacgt gccgctcacc cccggcggta
     9481 agctgacgtt cgaagacggc atttctgcac cgggccgcta cgtggagctc gaggcgcagg
     9541 tggacgtgcg cattttgatc tccaattgtc cgcagctcaa caacccctgc aatgcttata
     9601 accctactcc gattcaagtt ctaatctgga acgcgccgag ctaggactcg gccagaaact
     9661 cttctacgaa gccctctaac gacaacgtga gtgcatcgcc gtggcccggc tcctgcgcga
     9721 gtgggccccc aaagagccat gttcgaaagt gtattgattg ccaatcgcgg tgagatcgct
     9781 tgtcgcattt cgaggacgct gcgggcgctc ggcgcgcgtg tgatcgcggt ctattcggat
     9841 gccgatcgca agtcgcgtca cgttcgtgag gcggatctgg cgattcgctt gggcccggcg
     9901 ccagcccgag aaagctactt gaatgccgaa cgggttttga tggccgcccg tgagagcgga
     9961 gcccaagcca ttcaccccgg ctatggcttc ttgagcgaga atgccgactt cgcccaggcg
    10021 gtggaggcgg cggggctcgc gttcattggt cccacgccgg atcaaatccg tgccttcggc
    10081 atcaagcacc gcgcgcggga gctggccgaa cgcgccggag tgccgttgct gccaggcagc
    10141 ggcgcgctcg atgatttgag ccatgcggtg cgtgaagccg agcgtgtcgg cttccccgtg
    10201 atgctgaaga gctcggccgg cggaggcggt atcggcatgc agttggttcg agaaccggcc
    10261 gcgctcccca aagcctacga gagcgtacgc ttcgccgggc gcagcaactt cggcaatgcc
    10321 gcgctgttct tggagcggtt cgtagagcat gcgcggcaca tcgaggttca ggtgttcggc
    10381 gatggcgctg gcgccgtcgt cacgctcggc gagcgggatt gctcggcgca acgccgcaac
    10441 caaaaggtca tcgaagaggc accggcgccg aacctgcccc gcaaggtgcg cgaagcccta
    10501 cacggcgccg cgcgggaact actgagctcc gtcagctatc gctcggccgg aacggtggag
    10561 ttcatctacg acgccgagtg cgagcaattc tactttctcg aggtcaacac gcgcttgcag
    10621 gtggaacacg gagtcaccga attggtgcga ggcatcgact tggtggagtg gatggtacgt
    10681 acggccgcgg gggatgcgcc ggatctggct tcgctccctc gagagtcggg gcacgccatc
    10741 gaagtacggc tctacgccga agatcccgca cgcgagttcc ggccgagctc gggcctgctc
    10801 acgcagctgg agtttcccca gaacgcgcgc gtagacacat tcctcgaagt gggcgacgaa
    10861 gtggcctcgg attacgaccc gatgctcgcc aagatcatgg cgcacggcgc cgatcgagac
    10921 ggagccctat cggctctgga aagctctctg gcggagacgc gcatcggcgg cattcgcacc
    10981 aacctagatt acttgaaggc tctgctcacc tcgccgaccg tgagacaggg ccgcgtgttg
    11041 actcggaccc tgcctcactt tgcgtatgcg gatggcggtg tagagatcat tgcgccgggt
    11101 accttcagta tcttggtcga ctatcccggg cgaactggct attggagcgt gggagttccg
    11161 ccttcgggcc caatggatga ttggtcggcg cggattgcca atggcatcgt cggcaatccc
    11221 gaggattgcg cggttttgga gatgacgcac accggtgcga cgctgcgctt ttcggcagat
    11281 cagtgcatcg ccctcacggg cgcgcgcatg cgggcgaccc tcgatgggaa gtccgtatcc
    11341 tacgggcgcc cgctcgaagt gcgcgcgggc caggtcctcc aactgggcag tctcgaaggc
    11401 ggcggccaac gcagctacct ggccattcgc ggaggtttcg acgtaccgag ctaccttggc
    11461 agccgcacca ccttcacctt gggcggcttc ggtggccatg ctgggcgctg cctgcgcaaa
    11521 ggcgacgtct tgttcgcgca ggctcgcacc gatctgcgac cggttcgaga ggcgcccatc
    11581 gagtttctgc ccgcgttcgg taaaacttgg gagatccgcg tcatgtacgg cccgcacgga
    11641 gcacccgagt tcttcactcg acgcgacatc gacaccttgt tttcggccga gtatgaagtg
    11701 cactacaact cgagccgcac gggagttcgt ctcctcggac ccaagccaga gtgggctcgc
    11761 cccgacggag gagaggccgg gctacatcca tccaacatcc acgacgtcgc gtatgcgatt
    11821 ggagcgatcg acttcacggg cgacatgccc atcctcctcg gccctgatgg cccgagcctt
    11881 ggcggcttcg tctgccccgc cactattccc caagccgagc tgtggaaaat gggccaactc
    11941 catcccggcg atcgcgtccg cttcatcccc atcgccctcg aaaccgccct caccctcgaa
    12001 caccaacaac gcgccgccct cacccacttc ccttccctaa ccccgcttcc cgccgccgct
    12061 cccaactcga cctcaccaac tcaagctccc gccccaactt cggaagctca aacccccgcc
    12121 cccacctcgc aagctcgagc tccagacccg tcgcaagcgg ggctggggcc ccgcgcgcag
    12181 cgcggagcgc cttgggaggg gggccccgcc gcgggaggtc tggaacagac cctccaaaca
    12241 atacagcaga cgctcccaat aacgatacgc cccgccggag accgctacct cctcgtcgaa
    12301 tacgggccgt tggtcctcga cctgcgcctt cgattccggg tccacgcatt gatgacctgg
    12361 ctcgagcgag catcactccc gggagtactc gatctcacgc cgggcgtccg ctcgctccag
    12421 attcactatg acccggcgcg cctcccagcc gcgcaactcc gttcgctgat cgagcgcgcc
    12481 gaggacgagc ttggtgacgc gagcgacgtc gcggtgccca cgcgcacggt ttggctgccg
    12541 ttgtcctggg atgatcccga gacgcggcgc gcgatcgaga aatataccca gctggtgcga
    12601 gcggacgcgc cttggtgccc gagcaacatc gaattcatca gacgtatcaa tggcctcgcg
    12661 gacgaacgcg ccgtctacga taccgtgttc aacgccagtt acttggtgct cggcttgggc
    12721 gacgtctatt tgggagcacc agtcgcaacc ccgctggacc cgcgccaccg cctcgtaacg
    12781 accaagtaca acccggctcg cacttggacc cccgagaatg cggtcggcat cggaggtgcc
    12841 tacatgtgca tctacggcat ggaaggccca gggggctacc agttcgtcgg gcgcactttg
    12901 caggtttaca atcgattccg cacgacgcca gagttcgcgc ccggaacccc atggctgctg
    12961 cgcttcttcg accagattcg cttctatccc gtgagcgcca aggagctctt ggagctgcgc
    13021 gagagcttcc ttcacggcgc gcattcgatt cggatcgaac ccggcacgtt ccggctctcg
    13081 gagtaccaaa cgtttctgga agataatcag cgctcgatcg cgctgttcaa agcccaacaa
    13141 cagcgcgcct tcgaggccga acgcgctcgc tgggccgagc aagcgctcgc cgagccgagt
    13201 gaagaggcga gtatctctgc agccgtcccg gccaacctgg acgccctgcc cgagggtgtg
    13261 gtctcgatcg atagccacgt tacgggaagc atctggaagc tcgccgtgga gcgcggcgcg
    13321 cgcgtcaaga agggcgacgt gctggtcgta ctcgagtcca tgaagatgga gatctccgtg
    13381 gaggcgagcc acgacggaac ggtgaccgaa ctctcggtcg gggagggccg cgctgtgtcg
    13441 gccggtcaac gcctgatgtt gatcgagcaa gcggaggcgt catgaacgac ctcagtccac
    13501 gtcacttggc gcgggcgtat gcagctcgcg aaagagaccc gcaacaggtg ctgaacgagg
    13561 tctacgaccg tatcgcacga aatgggtcga gcccggtttg gactcatgtg aggccctacg
    13621 agagcttact ccgcgatctc gagtttgcag aggccaatat ccgcccggac gctcgtggcc
    13681 cccttttcgg cgtccccttt gccgtg
//
LOCUS       DASTKU010000970         8411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_412054, whole genome shotgun sequence.
ACCESSION   DASTKU010000970 DASTKU010000000
VERSION     DASTKU010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8411
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412054"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="VFQ61_34445"
     CDS             <1..1178
                     /locus_tag="VFQ61_34445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET9959660.1"
                     /translation="VRRKLDKTWSSESKVRSLVPRDSFLGERIVWQGQPEVTAPPAFL
                     RALAAVCFVTAVISGCFGAVIAVALAEFPLASVLFSLWCSGLGVALLVLPRRFAKTAR
                     YFVTDRRVIVKYGPFSRSIERRAISFARIFWTDEAAHTGDLEVVRAVPAGALRRRLRL
                     RLAGLIAPDRVWAIIRGAEDVAPAGQGERPVGQRLDHGESVIWTARPRPELKRFIPQG
                     RREWALLALSAFLLAGVGRIVRRALPVLARLSDDTGPARTWAIVGLALGLGLALCLMV
                     VIAAYLVYDAVIRQGILAGDTRYLVTNKRVLIQRRTEELHLDREKIIDVVDAPAGHGL
                     RNVFLVLDGPRARAVAVSGAFGEMTRGPHLRPVFEAVTDAESVSRILRDRLGLKAAA"
     gene            complement(1162..2136)
                     /locus_tag="VFQ61_34450"
     CDS             complement(1162..2136)
                     /locus_tag="VFQ61_34450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959661.1"
                     /translation="MGQTVLGVLALILVVSTVHAPVRAEPSIWANARDPMARRTDRVL
                     ARIERTWDMVSQAERDPSLVQDLRRDTLAIAETSGARAIDDPRVTYLLARVLSETDPT
                     REEEVSALLARARRNTGADQAWLRAEIDATWAESTQNDPRLAIARATRALAQQADPAR
                     CASLHRKRSDAKLALGDLRGAAEDARRAVARATSASDRSSAHALLAVVLDRSGDTERA
                     LYEMRLARWTAPEPELVGLWEVFVFRPHDLHYARALSFQAESRNADDEEETHAALALA
                     LDAWDHYLTDAPSDDAFRTLAQAHRAACARALERMQGDALDEALKQPL"
     gene            complement(2169..3185)
                     /locus_tag="VFQ61_34455"
     CDS             complement(2169..3185)
                     /locus_tag="VFQ61_34455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959662.1"
                     /translation="MMWPALLLLAATSSAGPPLMAYAGSSPRPAECRSSADSGELWEQ
                     ARGQAARRFCLLLARAYARLASAPGEALELARKAEALQPAAVEPRVVQGRAQLRLGDW
                     DGAYTALASSVLQAGRPLGDVYALRELAVAAAQTGRLAEARSAYRSLVSRAGFIEEPA
                     FAKRIALEAAPVLLQGNEADVSDAIVFLNEARRGVAVPGLSDLLVAELALCLDRAGKA
                     EDAEIVLKELNGAWSLTRWFSERERVLLSKSLDHSGQALPKAEFNPLAPMLLEPELFA
                     IIGYAAHITDPRLARTHLKAYLQAGGRAQDWARRQLQALGGESAPPAVPNASGGRGRN
                     PARP"
     gene            complement(3355..4653)
                     /gene="hisZ"
                     /locus_tag="VFQ61_34460"
     CDS             complement(3355..4653)
                     /gene="hisZ"
                     /locus_tag="VFQ61_34460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET9959663.1"
                     /translation="MSSTGALASGASRNAPGALAHPVPAGMRDLLPEEAARHLQLGRK
                     MLRAFELRGYELVSVPLFEFSDVVERGAGALEAHEVLRFVEPESGSVVSLRPDMTPQI
                     ARLVATRLAHVPGPVRLCYQGSVLRRRHERARRHRQIPQVGLELIGEGEPEGDLEVLS
                     AAAGAVRATGLSDFVIDLGHAGVATALVEPLEPARAREILDALEVKDEREVARRAERY
                     GLDSARRAALAELPLLHGGVEVFARAERALGATPAAPALQELKRLFEAALRAELAPSI
                     LVDLGETRDFEYYTGPLFQIHAEGPGSALGSGGRYDGLLARFGAARPAVGFALRLDDV
                     AWALEAAGLAAQAPVRVLLIGQAGEQVSALHAQDLVAAQAPEHDALAYAKAFRFSHCL
                     GRDGNGWLLQDVRSGNQSLFGLDEFPRLVAQLKALDTPPL"
     gene            4870..5628
                     /locus_tag="VFQ61_34465"
     CDS             4870..5628
                     /locus_tag="VFQ61_34465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HET9959664.1"
                     /translation="MTSHPTHHQEATKVDVCEHGNDAEGRPISLDRRLFMQLVAFECR
                     DELPAPHAKEALATALEASGLGGALYEDINHPRGFALLTFSEQPLDFVDKLRPALAYN
                     CPASLAVRPEFGMLGRTYSTGFEQNLAFWLLERPKKTVTNPAWPFAVWYPLRRKGGFD
                     RMDPKEKGGIMREHGGIGRAYGEADLAHDVRLACHGLDQNDNDFVIGLIGASLHPLSH
                     VVQAMRQTRQTAEFMEKMGPFFVGRAVVQKPGPQ"
     gene            5649..6329
                     /locus_tag="VFQ61_34470"
     CDS             5649..6329
                     /locus_tag="VFQ61_34470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET9959665.1"
                     /translation="MKLEAVRGIVFDLDGTLIDSRADIARAARHALLAFGFADLPDEE
                     IHGYVGDGARKLLARAARVELDDSRMNALYAAFIEYYAAHPVDYTEFCPGAREALALD
                     YGVPLALCTNKPRVTTACILRELGISERFAVVVAGDDLAKNKPDPLPLLHIAERLEAA
                     PQRLVMVGDGAQDVGCGRAAGARTIGLRGGIQPEARLLAAGADLVLGSLHELPRALSR
                     LDREMTAT"
     gene            complement(6342..8288)
                     /locus_tag="VFQ61_34475"
     CDS             complement(6342..8288)
                     /locus_tag="VFQ61_34475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9959666.1"
                     /translation="MNLAIALSIAACAVEFVIGGLALALASVPGFGHLRKFTWVALTA
                     ALYSAGDVCFAWPAVGEQVLPWLGRVNSAAACLHGIAWMVYSRAQYGSPLRRHDKLLM
                     AMLAWLAILSLLPGLVVGDHIEVQHVEWAHVTYRIPAAGPFGYALMPAAMLALINPGL
                     FYWRMARAGAPGARAHLLGFAVLFVLGINEVLVSLQVISSLYAADVGFLAVVLSVTGE
                     MTYRVAGDALRLQEFSRELSRQVEARTRELSETRDNLVRAERLSALGRLSASIGHEIN
                     NPLSYVIGNIEFARSELERGGDLPQVLEALRDAGSGADRIRRIVRELKAFSRGSDTEQ
                     CRVVDAREVIDAASKLVWGELRHRAELIVDLPPIAKVFIDPTRLTQVFVNLLVNAAQA
                     IPEQRAGIRSGLIRVRARNRENDVVIEVVDNGVGISPESRPHLFEPFFSTKPPDQGTG
                     LGLFVSLGIVRAAGGQIEVESEVGQGTTVRVVLPAHGGQEPEPERSRPPPSLRERRIL
                     IVDDDVLVARTLARLLRDQQVEIVANGQAALDRLARHSARQPEDSSSEVATDSAKDSA
                     NQPWDLVLCDLMMPGMTGMELDERVRGNYPKLAQRFVFISGGGVTERAREFIREHSER
                     VLSKPIDRTELLQVLARCVAEVSS"
BASE COUNT         1477 a         2827 c         2682 g         1425 t
ORIGIN      
        1 cggtcaggcg caagttagat aagacttggt cgtcagagag taaggtgagg tccttggttc
       61 cccgcgattc gttcttgggt gagcgcatcg tctggcaggg tcagccggaa gtcaccgcac
      121 cccctgcatt tttgagagcg ctcgccgcag tatgcttcgt gacggcagtc atcagtggct
      181 gctttggcgc cgtcatcgcc gtagcactcg ctgagttccc gttagcgtca gtgctcttct
      241 ctttatggtg ttctgggctg ggcgtggccc tgctcgtttt gccgcgtcgc ttcgcgaaaa
      301 cagcacggta cttcgtcacg gatcgccgcg tgatcgtgaa gtacggtcct ttttcgcgca
      361 gcatcgagcg cagagcgatc agcttcgcgc gcatcttttg gacggatgag gctgcccaca
      421 ctggggatct cgaggtggta cgtgccgtcc cagccggggc gcttcgtcgg cggcttcgtt
      481 tgcggctggc tggattgatc gcacccgatc gtgtttgggc cataatccgc ggcgccgagg
      541 atgtggcgcc cgcgggccaa ggtgaacgac cggttgggca gcgtctcgat cacggagaga
      601 gcgtgatttg gacggcgcgt ccgcgtccgg agctgaagcg cttcatcccg cagggtcgta
      661 gagagtgggc tcttttggcg ctctctgcgt tcttgctggc gggcgtgggc cgcatcgtac
      721 ggcgagcctt gcccgtgctg gcgcggctct cggacgacac gggcccggcc aggacttggg
      781 caatcgtggg gttagccctc gggctcggct tggcactctg cctgatggtc gtgatcgcag
      841 cttatttggt gtacgacgcc gtgatccggc aaggcattct ggcgggtgac acgcgctacc
      901 tggtcaccaa caaacgagta ctcattcaac gtcgcacgga ggagctgcac ctcgatcgtg
      961 aaaagatcat cgacgtggtc gatgctcccg cgggacacgg cttgcgcaac gtgtttttgg
     1021 tgctggacgg cccgcgtgcc cgcgccgtcg cggtcagcgg cgcgttcggg gagatgacgc
     1081 gcggtccgca cctgcgcccc gtattcgagg cggtcacgga cgccgagagc gtgagccgca
     1141 tcttacgtga tcggctgggc ctcaaagcgg ctgcttgagc gcctcgtcga gggcgtcacc
     1201 ctgcatgcgc tccaacgcgc gcgcgcaggc tgctcggtga gcttgcgcca aagtgcgaaa
     1261 ggcatcgtcg ctcggagcgt ccgtcaagta gtgatcccag gcatcgagcg cgagcgccaa
     1321 agccgcatgc gtttcctcct cgtcgtctgc gttccgcgac tcggcttgga acgacaacgc
     1381 acgggcgtag tgcagatcat ggggcctaaa tacgaagact tcccagagtc caacgagctc
     1441 gggttcggga gccgtccagc gtgccaacct catttcgtag agcgcacgct ccgtatcgcc
     1501 gctgcgatcg agcactacgg ccaggagcgc atgcgcactg gagcgatcgc tcgcgctcgt
     1561 agctcgcgcc accgcgcgcc gcgcgtcctc ggccgcccct cgcaaatccc cgagcgcgag
     1621 cttcgcgtca ctacgctttc gatgaaggct cgcacaacgc gcggggtcgg cctgctgcgc
     1681 tagggcccgg gtggcgcgag cgatggcgag ccgcgggtcg ttctgtgtgc tctcggccca
     1741 ggtagcgtcg atttccgcgc gcagccaagc ctgatcggcg cccgtattcc ggcgcgcccg
     1801 cgccagcaag gcacttacct cttcctcgcg ggtaggatcg gtttcggaga gcacacgcgc
     1861 cagcaagtac gtgacgcgcg gatcgtcgat cgcgcgcgca ccgctcgttt cagcaatggc
     1921 caaggtgtcc cgtcgtaggt cctgcaccag cgaagggtcg cgctcggcct gactcaccat
     1981 gtcccaggtt cgttcgatac gcgccaacac gcggtccgtg cggcgggcca tcgggtctct
     2041 cgcgttcgcc caaatcgatg gctcggcacg aacgggggcg tgcacggtgg agacgaccag
     2101 aatcaacgcg agtacaccga gaacggtctg gcccaccgcg ccaactcccc gaaccctgcg
     2161 cttgcagctc acggccgcgc aggattcctc ccccgaccac cggaggcatt cggtacagcg
     2221 ggaggagcgc tctcgcctcc gagcgcctgc agctggcggc gcgcccaatc ttgcgcgcga
     2281 ccaccagcct gcaggtaggc cttcaagtga gttcgcgcca gccggggatc ggtgatgtgc
     2341 gctgcatacc cgatgatggc gaagagctcg ggttccagga gcatcggggc cagcggattg
     2401 aattcggcct tgggcagggc ttggccggaa tgatcgaggc tcttcgagag caacacgcgc
     2461 tctcgctcgc tgaaccaacg agtgagcgac cacgctccgt tcagttcttt gagcacgatc
     2521 tcggcatctt cggccttgcc ggcgcgatcg aggcaaagag ccagttccgc gaccagcaga
     2581 tccgacagcc ccggcacggc cacaccgcgt cgagcctcgt tcaggaatac gatcgcatcg
     2641 gagacgtccg cctcgttgcc ctgcaacagt accggtgcgg cctccagcgc gatgcgcttc
     2701 gcgaacgctg gctcttcgat gaaacccgcg cgggacacga gtgagcgata cgccgagcga
     2761 gcttctgcga gccgacccgt ttgcgcggcc gcaaccgcga gctcccgcaa cgcgtaaaca
     2821 tcaccgagcg gtcgccccgc ttggagtacg ctgctcgcca gagcggtgta agcgccgtcc
     2881 cagtcgccca gcctcaattg cgcgcgccct tgcacgacgc gcggctctac cgcggcgggc
     2941 tggagcgcct cggccttgcg cgcgagttcc aaggcttccc caggcgcgct cgcgagtctg
     3001 gcgtaagcgc gggcgagtaa gaggcaaaaa cgccgggcgg cttggccacg tgcttgctcc
     3061 caaagctccc ctgagtcggc cgagctacgg cactcggccg gacgcggact cgagcctgcg
     3121 taggccatca atgggggacc agcggacgaa gtcgccgcga gaagcagcag agcgggccac
     3181 atcacagctg cgcataggtt agcactcgtg ctcgacgggg ccggtggctg cctgccatac
     3241 gcagcccaga ccaccgcctt accgtctcga aaccgcgggt tctgcgcggc agggccgcga
     3301 cgaaggggcg cgcccgaggg cgcgaagctg ccgctcagcc gctgccggat aacgtcagag
     3361 cggcggggta tcgagcgcct tcagttgcgc caccagcctc ggaaactcgt ccaagccgaa
     3421 gagtgattgg ttcccgctcc gcacgtcttg gagtagccag ccgttgccgt cgcggccgag
     3481 gcagtgactg aacctgaaag ccttggcgta ggcgagcgcg tcgtgctcgg gagcttgcgc
     3541 ggcaacgagg tcttgggcgt gcagcgctga aacctgttcg ccagcctgac caatcagcaa
     3601 cacacgcacc ggcgcttggg cggcaagccc cgccgcttcg agcgcccaag ccacatcgtc
     3661 gaggcgaagc gcgaagccca ccgcggggcg cgccgcgcca aagcgcgcca gcaagccgtc
     3721 gtagcgcccc cccgaaccga gcgcgctgcc gggcccttcg gcgtgaatct gaaacagcgg
     3781 ccccgtgtag tactcgaaat cgcgcgtctc accgagatct accaaaatgc tcggcgcgag
     3841 ttcggcgcga agagcggctt cgaaaagacg cttcaactcc tgcagcgcag gagctgccgg
     3901 tgtagccccc agcgcgcgct ccgcgcgtgc aaacacctcc acgcctccgt gcagcaacgg
     3961 gagttccgcc agggcggcgc ggcgagccga atccaaaccg tagcgctccg cgcgacgcgc
     4021 cacctcgcgc tcgtctttca cctcgagggc atccaggatc tcacgtgctc gcgccggctc
     4081 gagcggttcg accaacgccg tcgccacgcc ggcgtggccc aggtcgatga cgaagtcgct
     4141 cagcccagta gcgcgcaccg cgcccgccgc cgcgctcaac acttccaaat cgccttcggg
     4201 ttcaccttcc ccgatcagct cgagaccgac ctgcgggatt tgacggtgcc gtcgggcgcg
     4261 ctcgtgcctg cgccggagga ccgagccctg atagcaaaga cggaccggcc ccggaacgtg
     4321 cgccagacgg gtggccacta gccgcgcgat ttgcggcgtc atgtccggac gcaacgagac
     4381 gaccgaaccg ctctcgggct ccacgaagcg aagtacttcg tgagcctcga gcgcccccgc
     4441 gccgcgctcg accacgtccg agaattcgaa cagcggcacg ctgacgagct cgtagccgcg
     4501 cagctcgaac gcgcgcaaca tcttgcgccc gagttgcaaa tgccgagctg cctcctcggg
     4561 aagcagatcg cgcatgcccg cgggcacggg gtgcgccaac gctccgggtg cgttgcgcga
     4621 cgctccgctc gcgagcgcac cggtcgagga caagctagtc gaatgaaaat ccgcaggagg
     4681 gtacgagtgg ccggccaaag tcggcggagc ttatcaaaat tcgcggccaa agcgaagcga
     4741 gtcgctgagc ctcggcgatc cgtgcagcga gtgttaaggt tgcggcgcgc gttgctgttg
     4801 ccgtgccgaa atcgagccgg tccggctgag ctgccgtgca ctcgctgacg agccccaagg
     4861 agcccgacca tgacctccca tccgacccac catcaagaag cgaccaaagt tgatgtctgc
     4921 gagcacggca acgacgccga gggtcgcccg atttcgctgg atcggcggct gttcatgcaa
     4981 ctcgtggcgt tcgagtgccg cgacgagctg ccggcgcccc acgccaaaga ggctttggcg
     5041 accgcgctcg aggcctccgg cttgggcggc gcgctgtatg aagacatcaa tcatcccaga
     5101 gggtttgcgc tcttgacgtt cagcgaacag cccttggact tcgtcgacaa gttgcggccc
     5161 gccttggctt acaactgtcc cgccagcctt gcggtgcgcc cggagttcgg aatgctcggg
     5221 cgaacctact ccacgggctt cgagcaaaac ctggcttttt ggttgctgga acggcccaag
     5281 aagacggtca ccaaccctgc ctggccgttc gcagtctggt accccttgcg ccgcaagggt
     5341 gggttcgatc gcatggatcc aaaggaaaaa ggcggcatca tgcgtgagca cggcggaatc
     5401 ggtcgcgctt acggggaggc cgatctggcc cacgacgtgc gcctcgcgtg tcacgggctg
     5461 gaccagaacg acaacgactt cgtgatcggg ctgatcggtg cctccctgca tcccctctcc
     5521 cacgtagtgc aggccatgcg ccagacgcga cagaccgcag agttcatgga gaagatgggg
     5581 cccttcttcg tgggccgtgc agtggttcaa aaacccggtc cccagtgatc gagcccaggg
     5641 cagcgcacgt gaagctcgaa gccgttcgcg gcatcgtgtt cgacctcgac ggcacgttga
     5701 ttgattcgcg tgccgatatt gcccgagcgg cgcgccacgc gctgctcgct tttggcttcg
     5761 ccgatctgcc cgatgaagag attcacggct atgtcgggga tggcgcacgc aagctgctcg
     5821 cgcgcgccgc tcgtgtcgag ctcgacgatt cgcgcatgaa cgcgctgtac gccgcgttca
     5881 tcgaatacta cgcggcacat cccgtcgact ataccgagtt ctgccccggg gcgcgcgaag
     5941 ccctcgcgct cgactatggt gtgccgttgg cgctatgcac caacaagccg cgcgtcacga
     6001 cggcgtgtat tctccgcgag ctcggcatct ccgagcgctt tgcggtggtg gtagcgggcg
     6061 acgatttggc gaaaaacaag cctgatcctt tgccactctt acacatcgcc gagcgactcg
     6121 aggccgcgcc acagcgattg gtaatggtcg gagacggcgc gcaagacgtc ggttgtggcc
     6181 gcgccgccgg ggcgcgcacc atcggtttgc gcggcggcat tcagcccgag gcgcggctgc
     6241 tcgccgccgg cgcagatctc gtcctcggca gcctccacga gctgccacga gcgctctccc
     6301 gtctcgatcg ggaaatgacg gccacctgaa ctggcggtcg gctacgacga gacttccgcc
     6361 acgcaacgcg ccaggacctg cagcagttca gtccgatcga tgggcttcga gagcacccgc
     6421 tccgaatgct cgcggatgaa ctcgcgtgct cgctcggtga cgcccccgcc gctgatgaac
     6481 acgaacctct gcgcgagttt cgggtagttt ccgcgtaccc gttcgtccaa ctccatgccc
     6541 gtcatgcccg gcatcatcag gtcacagagc actaggtccc aaggctgatt cgcggagtcc
     6601 ttcgcactgt cggtggcaac ctcactgctc gagtcttcgg gttggcgcgc cgaatggcga
     6661 gccaaccgat ccagggcggc ctgaccgttg gctacgattt cgacctgttg gtcacggagc
     6721 agtcgcgcca gagtgcgagc gaccaacacg tcatcgtcga cgatcaggat gcgtcgctcg
     6781 cggagactgg gcggtggccg tgaacgttcc ggctcgggct cctgccctcc gtgggcaggc
     6841 aacacgactc tgacggtcgt gccttgcccc acttcgctct ccacttcgat ctgcccaccc
     6901 gcggcgcgca cgatgccgag tgagacgaac agcccgagcc cagtcccctg gtccggtggt
     6961 ttggtcgaga agaatggctc gaacaggtgg ggacggctct cggggctgat gccaacgcca
     7021 ttgtccacga cctcgatcac cacatcgttt tcgcgattcc ttgcccgcac tcgaatcaaa
     7081 cccgatcgga tgccagcacg ttgttccggg atggcttgag cggcgttgac caacaggttg
     7141 acgaacacct gggtgagccg cgtcgggtcg atgaacactt tcgcaatcgg tggcaggtcc
     7201 acaatgagtt ccgcacgatg acgcagctcc ccccacacga gcttgctcgc ggcatctatg
     7261 acctcacgag catctacgac gcggcactgt tccgtgtccg agcctcggct gaaggccttc
     7321 aattcacgga caattcgccg aatgcggtcg gcgccgctcc ctgcgtcgcg taaagcttcg
     7381 agcacctgtg gcaggtcccc gccccgctct agctcgctcc gcgcgaactc gatgttgccg
     7441 attacgtacg acagcgggtt gttgatctca tgtccgatac tggcgctcag gcggcccagg
     7501 gccgagagcc gctccgcccg cacgagattg tcacgtgttt ccgagagctc ccgcgtcctc
     7561 gcttccactt ggcgggagag ctctcgagag aactcctgga gacgtagagc gtcgcccgcc
     7621 accctatagg tcatctcccc cgtcaccgac aggactacag ccaaaaagcc gacatccgcg
     7681 gcatacaaac tggagatgac ttgcaaggag accaggacct cgttgatccc gagcacgaac
     7741 aacaccgcga atcccaacag atgagcgcgc gctccggggg cccccgcgcg tgccatccgc
     7801 caatagaata ggccaggatt gatcagcgcg agcatcgccg cgggcatcag ggcgtatcca
     7861 aacgggccag cggctggaat tcggtacgta acgtgcgccc actctacatg ttgaacctcg
     7921 atgtggtcgc caacgacgag cccgggaagc aggctgagga ttgccagcca agccaacatc
     7981 gccatcaaga gcttgtcgtg ccgacgcagg ggcgagccgt actgcgcgcg cgaatagacc
     8041 atccaggcaa tcccgtgcag acaggcggcg gcgctattca cgcggcccaa ccacggcaaa
     8101 acctgctccc ccaccgctgg ccacgcgaag catacgtcgc ccgcgctata cagcgccgcc
     8161 gtcagcgcca cccacgtgaa tttgcgcaga tgaccgaagc ccggaaccga agccaaggcc
     8221 agcgccagcc caccgatcac gaactcgacg gcacacgcgg caatcgatag agcgattgcg
     8281 aggttcatga cttcatcact ggcagggcgc agttggctcc cgatcctttg tgggcgagcc
     8341 gggaccaggc gcgcgccccg cccgggcgga gccgagcacc gagccctaac ccgcacggat
     8401 ggcggagtaa t
//
LOCUS       DASTKU010000971         9415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1531734, whole genome shotgun sequence.
ACCESSION   DASTKU010000971 DASTKU010000000
VERSION     DASTKU010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9415
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531734"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFQ61_34480"
     CDS             complement(<1..238)
                     /locus_tag="VFQ61_34480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959667.1"
                     /translation="MTPSGGSMLGTLFHRRFLQSAWPLMLFVSCTPLKTSARENPESL
                     ASPGGNANTMGLAECVAPTSPVVFTSLAGLSPDAR"
     gene            complement(235..1368)
                     /locus_tag="VFQ61_34485"
     CDS             complement(235..1368)
                     /locus_tag="VFQ61_34485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959668.1"
                     /translation="MSSAVLGRSVLARSMLGSTLRIGTLLTSAVLHTPVFLSPVLLGL
                     GACSSDVPPGALASSDSRAGHSPLGKDRPIVFRSSALEDGLGVLYRMAPDGSQLEAIT
                     SEGDITVPEWSPDGEQIAFRWNVEGVGSDVGILEPDHGAPVLLTRGENVFLRNSAVSW
                     SRDGTSVAFASAQERAGNKTFPDTWRMMTLERTGGTARPLLPSLAIGQEAAAWSPDGS
                     GSLVITQMGTTSAVDLWLVHGEAEPAPSNLTAGRVFAPRFPRWSPDSRQIVFAGYPLD
                     AEGNVEGLADHTHGFTPPDAEIFVVDVATQELTRVTDDEWDDTTPAWAPDGEHLLIAS
                     SRDGDTDLWLLPITAPEQARNLIDDADSPREDVMPSWFAGPTP"
     gene            1444..2991
                     /locus_tag="VFQ61_34490"
     CDS             1444..2991
                     /locus_tag="VFQ61_34490"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9959669.1"
                     /translation="MIGVLDSRMGQLIGGKYRVEGLLATGGMGLIYRARHQITGRAVA
                     LKLLRAELLGDSQITQRVSQEARLAVDATHPNVVEVLDAGCEETGIPYLVLERLFGQP
                     LDSLLSPKLSLVATSQVLIPIVNALVSLHERGIIHRDIKPSNIFLAWGPGAQLTPKLL
                     DFGIAKAVRESSITRSGVALGTPAYMAPEQALGRTASGPSADIWSIAVVLVKCLTGRL
                     PFDDSQSDSLKNSLSWERMAGIPAPLALVIERALQFEPADRFSDMITFRTEMLAALRE
                     IDPGVAWPSAETQSIAAEETELAALPALAQSRDSLDNAQQPTVIRDIVVPDTGIRDLA
                     IRDLEPPAAGQKADTRTLTSAHRALLETKLARILVTSSVLAILLGLGLLVFRSSSPTP
                     DTRALARTGKTRFDDPPDPRSSSAPTVPAEEAGAAPPLRGYATAANPVSPESSSIPRG
                     AVEPAGSVSRAPRSGSPHKPKAPKFGVPNNPQMSSPRALASATERVTPPRVDAGKLGA
                     NRAPILE"
     gene            complement(3020..4057)
                     /locus_tag="VFQ61_34495"
     CDS             complement(3020..4057)
                     /locus_tag="VFQ61_34495"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9959670.1"
                     /translation="MIVQLWVGVGGFAGWIEPASAADVAAPATTARSKSEEARECLAA
                     GVNARKRGQDHEAVGWFERALELDATPEAMAQAALAEQALGRWLKAHDRLVEALSESS
                     DPWIAEHADVLRSALQEIESHLGRLEVTCNVPGAEVRVDGYLIGVTPLASTRYVLAGQ
                     GVVEVSAPGFFSVARQVQVDVGSLSRVEVHLVRIQAGSGEFGSPQVGPRKPVVGSTEH
                     PRRVWAYASFGVAALGAGVGLAGSILREVNVRRYNDDSRCAVKLGIERSAECPAQVAS
                     ARQGEALAIAGYSAALLFGGAGLYLLLKSAPFQTTEPVNRAQPSNKADTTTGGWTCRI
                     GTSMVACARRF"
     gene            complement(4530..5906)
                     /locus_tag="VFQ61_34500"
     CDS             complement(4530..5906)
                     /locus_tag="VFQ61_34500"
                     /inference="COORDINATES: protein motif:HMM:NF002205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HET9959671.1"
                     /translation="MDWLELLCRKRVSHTGEVWETPRELGRSPFERDWDRILFSAAFR
                     RMHDKTQVFPLPDDDVVHSRLTHSLEVASVGRSLGQLVGRTVCERHAIDHERGFDFHD
                     VGDVVAAACLAHDIGNPPFGHAGEDAIAMYFRSAAGASAVQSLSERERADFESFEGNA
                     QGFRLLTRLQLEVDGGLQLTAAALAAFSKYPREAGVDLRQEGRTSRRKHGLFQSDTAV
                     FEVVARETGLHPTRSASGGRAWCRHPLAFLVEAADDICFSVLDIEDGVRLRVVDANEA
                     ETLLLPIARKAKAFSTERLQRFMEPKLRISYLRAIAISAMIEECVGAFLDVEPSILNG
                     SFDTSLADSIASRAELKALRELARSRCYNAAEVIEIELAGYEALGGLLEHFVPAVLSA
                     PEDTRRLPERDQKSLRLLRGRSVETGEPALYTRLLRVTDFVSGMTDRHALATYRRLKG
                     IALPGRAG"
     gene            complement(6536..7249)
                     /locus_tag="VFQ61_34505"
     CDS             complement(6536..7249)
                     /locus_tag="VFQ61_34505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9959672.1"
                     /translation="MHENKVALVTGANKGIGYAIACALAAHGLTVLVGARDAARGEAA
                     AQRIDGDARACPLDVTRADSIAAAVSRIQREIGRLDVLVNNAGIATFGTPSTTRLEDV
                     RAVFETNVFGVIAVTQAMLPLLRAAPAGRVVNVSTRLGSLSAITDPAFPMRGIGGVAY
                     GPSKSALNAITVAFAQELADTNIKVNAACPGHTATDLNGHSGARTADEAAKHPVRLAL
                     LGDDGPTGTFSNDTGTIPW"
     gene            7391..8128
                     /locus_tag="VFQ61_34510"
     CDS             7391..8128
                     /locus_tag="VFQ61_34510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9959673.1"
                     /translation="MSVPGLRALRWSSDELYVGMKESYTITRVEAGRSSFLTRGKVWS
                     CAPGSLRIQAPGDVVRDLARDGTATYQIVVFSPRAVDGVNDHVRIHSHLEAGDPRGAA
                     FQRLHDAVKVGAGRLALEVAIVEATSAFAAIQGARCEQTRAVRRAIEHLRECIDAEVT
                     LDSLADHAGTDKFHLCRAFRAQVGLPPHAYLTRLRIMRAKELLAAGAKPSEVAPQVGF
                     YDQSQLNRHFRRLVGTTPGAYAREHAT"
     gene            8528..9028
                     /locus_tag="VFQ61_34515"
     CDS             8528..9028
                     /locus_tag="VFQ61_34515"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HET9959674.1"
                     /translation="MVSCVAACSAAEEGGSAEDDLGTSKSPIAYVRSIQNGYGRWLDA
                     ELSTANRNGTKVHLWDWNGGSNQWWGFTWWGNDLWSIQSTYGPWRCLDAALESIDGNG
                     TKVQLWDCSFDTQQLWRLESGGQIVNARSGRCLDAEFPNINNLGTKVQLWDCSGGRHQ
                     SWTIGN"
BASE COUNT         1731 a         3005 c         3001 g         1678 t
ORIGIN      
        1 gccgcgcatc cggggaaagt ccggccagag acgtgaatac gactggtgac gtcggcgcca
       61 cgcattccgc aaggcccatc gtgttggcgt ttccgcctgg gctcgccaaa gattccgggt
      121 tctctcgcgc cgacgtcttg aggggagtgc acgaaacgaa cagcatcagg ggccaagcgc
      181 tctggagaaa cctgcgatgg aacaacgtgc ccagcatcga gccaccgctc ggggtcacgg
      241 tgtgggccca gcgaaccagc tgggcatcac gtcttcacga ggtgaatccg cgtcatcgat
      301 gagattccgg gcctgctccg gcgctgtaat gggcaagagc cacaaatccg tgtccccgtc
      361 ccggctgctt gcgatcagga ggtgttcgcc gtccggggcc cacgcgggag tcgtgtcgtc
      421 ccactcatcg tcggtcacac gcgtaagctc ttgggttgcg acgtcgacga cgaatatttc
      481 cgcatccggt ggagtgaagc cgtgcgtgtg atccgcaagc ccctccacgt tcccttctgc
      541 atcgagggga taacctgcaa atacgatctg gcgtgagtcc ggagaccaac gcggaaagcg
      601 gggagcaaag acgcgccccg ctgtcagatt cgaaggcgcc ggctctgcct cgccgtgcac
      661 tagccaaagg tctactgctg acgtggttcc catctgcgta atcaccaaag atccacttcc
      721 gtctggcgac cacgccgcgg cctcttgtcc gattgcgagt gacggtagta ggggccttgc
      781 agtcccgccc gtgcgctcca aagtcatcat gcgccaggta tccggaaacg tcttatttcc
      841 cgctcgctcc tgagccgaag caaacgccac gctcgtgcca tctcgcgacc aactcaccgc
      901 ggagttgcgc aaaaacacgt tttctcctcg tgtgagcaag accggtgcgc cgtgatctgg
      961 ttctaatatt ccaacgtcgg aaccgacccc ttcgacgttc cagcgaaagg caatttgctc
     1021 tccatctggc gaccactccg ggacggtaat atccccctca gaggtgatcg cctcgagttg
     1081 gctaccatcg ggagccatac ggtacagaac gcccagccca tcctccagtg cggaggaacg
     1141 aaacacgatg gggcgatctt tcccgagtgg cgagtgacca gcgcggctat cggacgaagc
     1201 gagcgcgccg ggcgggacat cgctactgca cgcgccgagg ccgagcaaca ctgggctgag
     1261 gaacactggg gtgtgcaaca ccgcgctcgt caataatgta ccgatccgca gggtactgcc
     1321 cagcatacta cgggccagca cgctgcggcc cagcacggca ctggacacgc tgcggacggg
     1381 cgctctggct tgcgggggtg acgacctgga agcgcggggg caaatcatgc taatgcgtat
     1441 cccgtgattg gagtgctgga ttcccggatg gggcagctga tcggcggtaa ataccgtgtc
     1501 gagggcttgc tggcaacggg cggcatgggt ttgatttatc gtgcgaggca ccagataacc
     1561 gggcgcgccg ttgcgctcaa attgttgcga gccgagctgc tgggcgattc acaaatcacc
     1621 cagcgcgttt cccaggaggc gcgcctggcg gtcgatgcca cacatcccaa cgtggtggaa
     1681 gttttggacg cgggctgcga ggaaaccggt atcccgtact tggttctcga gcggctcttt
     1741 gggcaaccgc tagatagctt gttgtctccc aaactgtctc tagttgcgac gagccaagtc
     1801 ctaatcccca tcgtgaatgc cttggtctca ctgcacgagc gcgggatcat ccatcgcgac
     1861 atcaaaccat ccaacatctt cttggcgtgg ggcccggggg cgcagctcac gccgaaactc
     1921 ttggactttg ggatcgcgaa agccgtgcgc gagtcgagta tcactcgctc cggggtggcg
     1981 ctcggaactc cggcatacat ggcgcccgag caggcgttgg gacgcaccgc gagcgggcct
     2041 agcgcagaca tctggtcgat cgccgtcgtc ctggtgaagt gcctgactgg gcgactacca
     2101 ttcgacgact cccaatcgga ttccctgaaa aactccctat cttgggagag aatggcggga
     2161 attcctgcgc cgctcgcgct cgtgatcgaa cgagctctgc agttcgagcc ggcggaccgc
     2221 ttctcggaca tgatcacctt tcgaacggag atgctggcgg cactccgaga aatcgacccc
     2281 ggcgttgcgt ggcccagcgc cgagacacaa agcattgctg cggaagagac cgaattggcg
     2341 gcgctgcccg cgctggccca atcgcgcgac tctttggaca atgcgcagca gccgaccgtc
     2401 attcgagaca tagtcgttcc tgataccggg attcgagacc tagcgattcg agacctcgag
     2461 ccacccgcgg ccggacagaa ggcggatact cgcaccctca cgagcgcgca ccgcgctttg
     2521 ctcgagacca agctggcgcg aatactggtg acctcgagtg tgctcgcgat tctgctcggg
     2581 ttgggtctac tcgtatttcg ctccagttct ccaactcccg atactcgagc cctcgctcgg
     2641 accggcaaga cacggttcga cgacccgccc gaccctcgat cgtccagtgc tcccactgtg
     2701 cctgccgagg aggctggcgc agcgccgcct ttgcgcggat atgccaccgc agcaaaccct
     2761 gtgagccccg aatcctcatc tatcccccgc ggggcggtcg agccagccgg ttcggtgtcg
     2821 cgggccccac gcagcggctc tccccacaag ccaaaggcgc cgaagttcgg ggtacccaat
     2881 aacccccaaa tgagctcccc tcgagctctg gcgagcgcca cggagcgtgt gactcccccc
     2941 cgggtcgatg cgggaaagct tggcgcaaat cgcgcaccga tcctggagtg aggctcgcga
     3001 gtcgtgcggc cattccagtt caaaaccgcc ttgcgcaagc gaccatcgaa gtaccgattc
     3061 gacacgtcca ccctcctgtc gtcgtgtctg ccttattaga gggctgcgcc ctattgacgg
     3121 gctccgtagt ctgaaagggc gccgacttca aaagcagata cagccccgcg ccgccgaata
     3181 acagcgccgc ggaataacct gcaatcgcga gcgcctcccc ctgacgtgcg gacgccacct
     3241 gggcggggca ctccgcggag cgctcgattc caagcttgac tgcgcagcgc gaatcatcgt
     3301 tgtagcgccg cacattcacc tcgcgtagga tcgagcccgc gagccccacg ccggctccga
     3361 gcgcagccac cccgaagctc gcgtacgccc agactcgtct cgggtgctcc gttgacccaa
     3421 caacaggttt ccgcggacca acctggggcg atccaaattc accagaccca gcctgaattc
     3481 gaaccaagtg aacctccacg cgcgaaagac tccccacgtc cacttgcacc tgtctcgcca
     3541 cgctgaagaa acctggagct gaaacctcga ccacaccttg cccggctagc acataacgag
     3601 ttgacgccag cggcgtaacc ccgatcaagt agccgtccac gcgcacctct gctcccggga
     3661 cattgcaagt cacctcgagt cttcccaaat gagactcaat ctcctgcagc gcgcttctga
     3721 gcacgtcagc atgctcggca atccacggat ctgaactctc actcaaggcc tccaccagtc
     3781 gatcgtgcgc cttcaaccaa cgtccgagcg cctgctcggc gagcgctgcc tgcgccatcg
     3841 cctcgggcgt tgcgtccaat tccagcgccc gctcgaacca gcccaccgct tcatggtctt
     3901 ggccccgctt gcgtgcattc acacctgctg ccaggcactc acgcgcctcc tccgacttgg
     3961 accgcgccgt cgtcgccggg gcggctacat cagcggccga tgctggctcg atccaccccg
     4021 cgaacccgcc cacgcctacc catagttgca cgatcacgca gagctgctct gcgaacagtc
     4081 cacgccgacc tcgcccggcg cgacatagcc tctgacggcg actgcacgaa actcctggag
     4141 acaaggctcg cgcgcgatac tcgcggcacc gcggacctgc gtctagtact agattgcgct
     4201 ttcgtcccat tgctcgacca tcgcggagcg gaccaccgaa cgccgcgctt tcctcgagtg
     4261 ccatgtgaac cgcggaagcc cgaaagccgt cgccgccccg ttcatatggc gcgcgagctc
     4321 gcgtcatata gcgcgcgagc tcacgcggat tcgcgtgcgg gccaccgcgt aaagtgcgcg
     4381 gcgttcgtca cagcacaagt cagcgccgta acgagccctc acagagcggc ataacaccgc
     4441 gcgggccccc gcaacaacgt gcttccgcaa ctcgagccag catgcgcaaa cctcgaatcc
     4501 ggcggagcac gccctaccgc gcgcgcggat tatccggcgc gtcccgggag cgcgatgccc
     4561 tttagacggc ggtaggtggc cagcgcgtgg cggtcggtca tgccgctgac gaagtcggtg
     4621 acgcgcagga gacgtgtgta gagggcgggt tcgccggttt cgaccgagcg cccacgcagg
     4681 agtcgtaaag atttttggtc gcgctcgggg aggcgacggg tgtcctccgg ggcagataga
     4741 acggcaggga cgaagtgttc gagtaggccg ccgagcgcct catatcccgc gagctcgatc
     4801 tcgatgactt cggcggcgtt gtagcatcga gaccgcgcga gttcgcggag ggccttgagt
     4861 tcggcgcgac ttgcgatgct gtccgccagc gatgtgtcga acgaaccatt caagatcgaa
     4921 ggttcgacgt cgaggaaggc tcccacgcat tcttcgatca tggcgctgat ggcgatcgcg
     4981 cgcaggtagc tgatgcgtag tttgggctcc atgaagcgct ggagccgctc ggtcgagaat
     5041 gctttggctt tgcgcgctat ggggagcagg agtgtttcgg cttcgttggc atcgacgacg
     5101 cgcagtcgca ctccgtcctc gatgtccagc accgagaagc aaatgtcgtc ggcggcctcg
     5161 accaagaacg cgagtggatg gcggcaccag gcgcgcccgc ctgacgccga ccgcgtcgga
     5221 tgcaatcctg tttcacgcgc gaccacttcg aatacggccg tgtcgctctg aaacagtccg
     5281 tgcttgcgac gtgaggtgcg gccttcttgt ctcagatcga cgccggcctc gcgcggatac
     5341 ttcgagaacg cagcgagtgc tgcggcagtg agctggagcc ctccgtccac ctcgagctga
     5401 agtcgggtga ggagccggaa gccctgcgcg ttgccctcga agctctcgaa gtctgctcgt
     5461 tctcgctcgg aaagtgactg gacggcactc gcccccgcag ccgagcgaaa gtacatcgcg
     5521 atcgcatctt cgccggcgtg accaaatgga gggtttccga tgtcgtgagc gagacaggcc
     5581 gcggctacga catctcccac gtcatggaag tcgaagccgc gctcgtgatc gatggcgtgg
     5641 cgctcgcaaa cggtgcgccc gacgagctgc cccagcgaac gcccgacgga cgccacctcg
     5701 agactgtggg tcaggcgtga atgcacgacg tcatcgtcgg gcagcgggaa cacctgggtt
     5761 ttgtcgtgca tgcgccggaa agcggcggaa aagaggatgc gatcccaatc gcgctcgaat
     5821 ggactgcgtc ccaactcgcg cggggtttcc cagacctcgc cggtgtgaga gacacgcttt
     5881 ctacacagca attcgagcca atccacgcgc gcagcctact acagaacccg cgttcccacg
     5941 ctactcggcg ccgtgaccgg gtggtccgcc cgcgcccgcc cgtcgaggag cgtgggctcg
     6001 cgcgtgaacg agcccgaggc cgagcgaccg ccccgtcgtt ccgcacgacg gtgacgaggt
     6061 ccaaacgcgc cttttagccc atcccattgc ggggctcagg tacgggcgtg gatggacgcc
     6121 gtcatagtgg ctggcccgcg attcgcgagc tccgcaagac agctccgtga tgagactggg
     6181 ttgcagagag ggccggcgcg gtgtggtgag ggatggcttg cttctctgtc agcgctaaag
     6241 ggggaagtcg acgcgcagcg cgcacgttta cggagtggcc cagccgaagc gtgcctcgat
     6301 ctcgattatc gagagtgaat cccgatggga gtggcgggcg tgtgcctcca gtttcggaag
     6361 gcaacggtgg caactcgagc tctgagccgt cgagtggagg cggggctcgt tccggcaatg
     6421 aggttggtgc gggtacgggg ctccgtccgg gcacggcgga cggggcacct aatcgaggat
     6481 actgcgtcag ggccgatcga tgtgcggaga cgcatcggca gccccggttc gcgtttcacc
     6541 acgggatggt accggtgtcg ttcgagaacg tgccggtcgg gccgtcgtcg cccagcaacg
     6601 cgagccgcac gggatgtttg gcggcctcgt cagccgtacg cgcaccgctg tggccattca
     6661 ggtcggttgc ggtgtgaccg gggcacgcgg cgttgacctt gatgttcgtg tcagcgagct
     6721 cctgtgcaaa tgccacggtg atggcgttga gtgcgctctt cgatgggccg taggcgactc
     6781 cgccgatgcc gcgcatcggg aacgcggggt ctgtgatggc gctgagcgag ccgaggcgag
     6841 tggagacatt cacgacgcgc cctgctggcg ccgcgcgcag gagaggtagc atggcctgag
     6901 tgacggcgat cacgccgaaa acgttggttt cgaacacggc gcgcacgtct tcgaggcgtg
     6961 tcgtgctcgg tgtgccgaac gttgcaatgc ccgcgttgtt gacgagcacg tcgaggcgcc
     7021 cgatctcgcg ctgaatgcgg ctgacggcgg cggcgatgga gtcggcgcgg gtgacgtcta
     7081 gggggcaagc gcgagcatcg ccgtcgatac gttgggcggc cgcttcgccg cgcgcggcgt
     7141 ctcgtgcgcc gaccaacacc gtgagaccgt gcgcggcgag agcgcacgcg atggcgtaac
     7201 caataccctt gttggcgccg gtgaccagcg cgaccttgtt ttcgtgcatg gttgggagat
     7261 agtcagcgag gcagtcgttg acttggagat tcttggcggc aaattcggcg aatcgcgcca
     7321 agaatctcca agcgtcccgc cgcgctttgc gtaaactggg cctgtggtgg cccgcaagca
     7381 tgtcctcccc gtgtccgttc ccggcctgcg cgcgctgcgt tggtcgagcg acgagctcta
     7441 cgtcggaatg aaggaatcgt acacgatcac ccgtgtcgag gcggggcgtt cctctttttt
     7501 gacccgcggc aaggtgtggt cgtgcgcgcc ggggtcgctc cgaatccagg cgcccggcga
     7561 cgtggtgcgc gacctcgctc gcgatggcac ggcgacgtat cagatcgtcg tgttttctcc
     7621 gcgtgcggtg gacggcgtca acgaccacgt ccgcatccat tctcatctcg aggcgggaga
     7681 tccgcgcggc gcggcgttcc aacgcttgca tgacgcggtg aaggtcggtg cgggtcggct
     7741 ggcgctggag gtcgccatcg tcgaggcaac ctctgcgttt gcagccatcc agggtgcgag
     7801 gtgcgagcag acgcgcgcag tgcgccgcgc gatcgagcac ctgagggaat gcatcgacgc
     7861 cgaggtgacg ctcgactcgc ttgccgatca cgctggcaca gacaagttcc acctctgccg
     7921 cgccttccgc gcgcaagtcg gcctaccacc tcatgcttac ctcacgcgcc tgcgcatcat
     7981 gcgcgccaaa gagctcctgg cagcgggcgc aaagccgagc gaggttgcac cgcaagtcgg
     8041 gttttacgac cagagccagc tgaaccggca cttccggcgc ctcgttggca cgacccccgg
     8101 cgcttacgca cgggagcacg cgacctagaa ttcgaccgag tcaacagtcc tgcagcttgg
     8161 ccatggcaga ctgggcacaa cgccgcgcga cctataatga cgttcgagtg gtgccggagc
     8221 atctcgttca acggcgtctc ctcgcgcgcg ccgccccaag cctaaggcgg cgaacgtgac
     8281 ggtcagttcg accgtggttg aggttggacc aggcgggccg tgatcgccgg acgcggcgaa
     8341 aaagtaacca cttagggtgc ggcgcgtgcg atggtgaggc gggtagagcc tggcgagcgc
     8401 ccggcaagag catcggtcgg aagttcatcc gccgaacagg gaagcattcg tctcggacag
     8461 cgctggcacc atcatgcaaa acagaaacaa ccgcacatta gttttttcgt taacaaggtt
     8521 agcgatcttg gtttcttgcg tggcggcttg ctctgccgcg gaggaaggcg gaagtgccga
     8581 ggatgacctc ggcacttcga agtcgccgat tgcgtacgtg aggtccatcc agaacggtta
     8641 tggccggtgg ctcgatgccg aactcagcac cgccaatcga aacggcacta aggtgcatct
     8701 ctgggattgg aacggtggca gcaatcagtg gtgggggttc acctggtggg gcaatgacct
     8761 gtggtcgatt caaagcacct atgggccgtg gcgctgtctc gatgcggcac tcgagagcat
     8821 tgacggtaac ggcacgaagg ttcagctgtg ggattgcagc ttcgacactc agcaactgtg
     8881 gcgtctcgaa agcggcgggc agatcgtgaa cgctcgcagt ggtagatgtc tagacgctga
     8941 gtttccgaac atcaacaacc tcgggaccaa agtgcagctg tgggactgca gcggcggccg
     9001 acatcagagc tggactatcg gcaactgact ctccgcagga ttcgcgggta agcgatgcgc
     9061 agcaggcgaa gcgttacggt gagggcaccg ccaccgaccg tggtcgccgc tgcgcctcct
     9121 cgtaaggcct cagggagggc gcgttgggtc gttttccggg ggtgtattcg ccgcgctcgc
     9181 tggtgcagcg ctcacgacca agatcgcgca gctcgccaac tcaagtccgg cgtagccagt
     9241 ccagagttcc gaacaatggc ttgggtgcgt tgcccaaggt tacccggcgc taacgaggac
     9301 gtagctcggg gacagtcttt cgatccctga acccctgtct ttcgcgtcga gaaggagctc
     9361 gcggcggccc tgaaattgga gcagccgctc aagccatttc caatcgcttt cggca
//
LOCUS       DASTKU010000972         4914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1308095, whole genome shotgun sequence.
ACCESSION   DASTKU010000972 DASTKU010000000
VERSION     DASTKU010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308095"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..493)
                     /locus_tag="VFQ61_34520"
     CDS             complement(179..493)
                     /locus_tag="VFQ61_34520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959675.1"
                     /translation="MGGAAVEVAAGGLEVAIRGGLEEPGPTFPGIGCLAVGLADDVVK
                     VVATTVLAGALDATPGMVIGFPIESSGTVPVSTARFCGLAACNTGLEGNRVGQGQKAA
                     EA"
     gene            complement(537..1520)
                     /locus_tag="VFQ61_34525"
     CDS             complement(537..1520)
                     /locus_tag="VFQ61_34525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959676.1"
                     /translation="MRNTRAAIATVVYCSLLPLGLRADPIRPPGPLGQGEAFVAGSSD
                     APSGHPAAPGEDSTPPPTPPGEPTPGEIEKLWAEASQLYSAGDYQAAYEKMQLYYQLT
                     RDARMLYNLGDLSRELARCSQALSHFEQYLEQAPDGSYRAQAQAAVMELREQCPPQLV
                     PSTQSGAPAAPRALVSPTTQTSVQTVRLSTEQPSEGLRQSSSDNARRYIGYGLIAGGA
                     ALGVGAGILIGSAIHKNNLVQSANRAAAEQPPDVRPIWSHDEEQLERERDRERNWAVV
                     LGVGSTLAFASGVATLLWPVREPHPASAPRTSLAIQPSARGLGLCLRVSSL"
     gene            1751..3610
                     /locus_tag="VFQ61_34530"
     CDS             1751..3610
                     /locus_tag="VFQ61_34530"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HET9959677.1"
                     /translation="MPLSFSILRLQSRSLQLLAASLPALLLGHFGCSDTINNHYYYGS
                     SGGAGGSGGSGGRGGSNAQTGGRVSTGGSSGGDSAAGAVNAGASNAGAANGGAAGAVN
                     TGGDSSGAGAPPTNGGEAGAGGTSELPSDYPDAPKANTSVAEHQLDLFGVVGARFWFG
                     VSAEQLEFMNEGLTGGGPIIINQLNGDIYTPGGSGATFADHVWITTAGENPRTADYGK
                     VQLKIVGQSTLRPWNKRSIPNLNIDADEFVDDQRIAGFEHLRLNNAQVGSIFREHLTL
                     EFYRRLGYPAPLSTFAWVSSNVWGKDVSVPYVLVERYKRAFCQREKDKLGGGCANMWE
                     FAGDFVNGGGGVPIPLDIAPPKGFPGQGPDVFDDPENCQIGKCENARVKELQTLLSDT
                     PLGEGFQAATADYVDWKEFQRFQCLSWMLGTGDDALHNQNNVVLVERSDGKFQYLPYS
                     VDISLGQDWYPSVTLAGQNLLARGCQSDKACWAETIATCEGLITDFTQMQPLKILDIV
                     HAELSDAGMLRGGDEARYEYIQAWLTTRLETLSSELEENRESPVLCQDGMIECNGFCA
                     PPEACGACVPPVFKRAAIDVEAAGADGVEPPGDVGGAPGGIDECPLKSEYQLR"
     gene            complement(3685..4167)
                     /locus_tag="VFQ61_34535"
     CDS             complement(3685..4167)
                     /locus_tag="VFQ61_34535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959678.1"
                     /translation="MNMERHRALVVLDPKHTAHVFHPVSDLAHVSQARENVNLLNAAV
                     RVENVKLPYGLDLAWTLRNVLGVFGGWVASRRFFEQERRVLAMWTTYRPEDGAQLFEQ
                     LCTDPVLRTHEDAWELSFRYFNFLGGVEDWSASGRTQQIDTYSARQLLPNRTFMPPVG
                     "
     gene            complement(4383..>4914)
                     /locus_tag="VFQ61_34540"
     CDS             complement(4383..>4914)
                     /locus_tag="VFQ61_34540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959679.1"
                     /translation="TDSDFPWCKKHHFWVATNTERRILLIVLDPAHVAHVFRPVTDLA
                     LASQARENVDLLNAVIRAEGVELPHGLDLAWALRNVICPLGGNVATRKFLEEQRKSLA
                     MWATYRPRDGAQLFEQCCTDPVLHTHEDVWELSFRYFNRAGGVEEWSAAGNRHQIVNY
                     SARQLLPNRTFMPPFF"
BASE COUNT          954 a         1539 c         1526 g          895 t
ORIGIN      
        1 agtgggacga gctcatcttg cgctgtctgg cacgggaacc caagcagcgt ttcggatcgg
       61 ctgacgaagt gctcgcggag ctggtccaag cgcctgtgac aacgggtccg tggcgtcgag
      121 gtgagctgat ttccgccgca aagtacctgc ttgccggaag ctgcgtcgcg ggagcgctct
      181 acgcctcggc agctttctgg ccctggccca cgcggttgcc ctccaagcct gtgttgcacg
      241 cggctagccc acagaaccga gcagtcgaaa ccgggacggt accgctcgat tcgatcggga
      301 agccaatgac catccccggt gtggcatcga gcgcgccggc gagcaccgtg gtggctacga
      361 ccttgaccac atcgtctgca agccccaccg ccaagcagcc aattccgggg aaagtagggc
      421 ccggttcctc gagcccgcca cggatcgcga cctccaatcc tcccgcggcc acctcgaccg
      481 ctgctccgcc caatgactct gcttccccca aagagccaaa gtgggagaag cctcgactac
      541 aacgacgaga ctcggagaca caaacccaag ccgcgcgcgc tcggctgaat ggcgagtgag
      601 gtccggggag cactcgctgg atgcggctcg cgaactggcc aaagcaaggt ggctacgccc
      661 gaagcgaaag ccaaagtgga acccacacca agaactacgg cccaattgcg ctctcgatct
      721 cgttcacgct ccagttgctc ttcgtcgtgg ctccagatgg gtcgaacgtc aggcggttgt
      781 tccgccgcgg cgcggttcgc tgattgaacg aggttgttct tgtgtatcgc ggagccgatc
      841 aggattcctg cccccacacc gagcgccgct cctccggcga ttagtccgta gccgatgtat
      901 cgtcgcgcgt tatccgagct ggattgccgg agtccctccg agggttgctc agtagaaagc
      961 ctcaccgtct ggacgctcgt ctgagtggtg ggtgaaacca gcgcccgcgg cgccgcgggc
     1021 gcgcccgact gggtactcgg cacgagctgc ggcgggcatt gttcacgaag ctccatgacc
     1081 gccgcttggg cctgcgcacg atacgagccg tccggcgctt gctcgagata ctgctcgaag
     1141 tgcgacagcg cttgtgaaca ccgtgcaagc tcgcggctga gatcgccgag attgtagagc
     1201 atgcgggcgt cgcgcgtgag ctggtaatac agctgcatct tctcgtaggc ggcctggtag
     1261 tcgcccgcag agtacagctg cgaagcctct gcccagagct tctcgatttc gcccggcgtt
     1321 ggctcacccg ggggagtggg cgggggcgtc gaatcctctc cgggcgcggc agggtggccg
     1381 ctaggcgcat cgctcgagcc ggccacgaat gcttctccct gccccagggg tccgggcgga
     1441 cgaatgggat cggctctcaa tcccaacggc agaagcgagc aatacacgac cgtcgcaatc
     1501 gcagctcgag tgttccgcat gatagccctc ttcgtggccc cgaaacatta agagtgcctg
     1561 cggattgggt caacgctcaa gggcgtctgc ttggcacagg gctgtgccac tttcagatcg
     1621 caatgtcgca gcattggcct gtcccagtaa gagccctacc caggaggagc gcccgagatg
     1681 gttgccgaga gtgaagcgcg cgaagctccg tatgcagctg gcatgccggt tgcccaatgg
     1741 ccttcgactc atgcctctca gcttctccat cctgcgttta caatctcgct ccctccagct
     1801 gctcgcggcg agcctacccg ctctgctgct cggtcacttc ggatgttccg acaccatcaa
     1861 caatcactat tactacgggt cctcgggcgg ggctggcggt tcgggcggta gcggcggaag
     1921 agggggaagc aacgcacaga cgggcggtcg cgtcagcacc ggcgggagca gcgggggtga
     1981 ctcggcggcg ggcgccgtaa acgcgggcgc ctcaaacgcc ggcgccgcaa acggcggcgc
     2041 cgctggcgcg gtcaacacgg gcggggattc ctcgggggcg ggagctccgc ccacgaatgg
     2101 cggagaggca ggagctggcg gaacgagcga gctgccgtcc gattaccccg atgcgcccaa
     2161 agcgaacacc agtgtcgcgg agcatcagct cgatttgttc ggagtagtcg gggctcgctt
     2221 ttggttcggt gtctcagcgg agcaactcga gttcatgaat gaaggactca cgggcggtgg
     2281 cccgatcatc atcaatcagt tgaacggtga catctatacg cccgggggca gcggcgcgac
     2341 tttcgcggat cacgtctgga ttacgacggc tggagagaac ccccgcaccg ccgactacgg
     2401 taaggtgcag ctgaagatcg ttggtcaatc gacgctccgc ccctggaaca aacgctcgat
     2461 tccaaacctc aacatcgatg ccgacgagtt cgtcgacgac cagcgcatcg cgggcttcga
     2521 acatctgcgg ctgaacaatg cccaggtggg gagtatcttc cgagagcact tgacgctcga
     2581 gttctatcga aggctcggct atccggctcc cctctcgacc tttgcctggg tgtccagcaa
     2641 tgtctggggc aaggacgtct ccgtgcctta cgtgctcgtg gaacgctaca agcgggcttt
     2701 ctgccagcgc gagaaggaca agctcggcgg cggctgcgcg aacatgtggg agttcgcggg
     2761 tgacttcgtg aacggcggcg gcggagtgcc gatcccgctc gatattgccc cgccgaaggg
     2821 atttcccggc caagggccgg atgtattcga cgatcccgag aactgccaaa tcggtaagtg
     2881 cgagaacgcg cgagtcaaag aactacaaac cttgctatca gacacgccgc tcggcgaagg
     2941 cttccaagcc gccaccgcgg attacgtcga ttggaaggag ttccaacgct ttcaatgcct
     3001 ctcgtggatg ctcggcaccg gcgatgatgc actccacaat cagaacaacg tggtgctggt
     3061 cgaacgcagc gacggcaagt tccagtatct gccttactcc gtagacatca gcttgggtca
     3121 ggattggtat ccgagcgtca ccctcgccgg tcaaaacctt cttgcccgcg gctgtcagag
     3181 cgacaaagcg tgttgggcgg agacgattgc cacctgtgag gggctcatca cggacttcac
     3241 ccagatgcag cctttgaaga tcctagacat cgtgcacgcc gagctctcgg acgcgggcat
     3301 gctgcgcggc ggggacgaag cccgttacga atacatccaa gcctggttga cgacccgcct
     3361 cgagaccctg tcgagcgagc tcgaagaaaa ccgcgagagc ccggtcctgt gtcaagacgg
     3421 catgatcgag tgcaacggct tttgcgcgcc gcccgaggcc tgcggcgcct gcgttccccc
     3481 agtcttcaag cgcgcggcga tcgacgttga ggctgcgggc gcggatgggg ttgagccgcc
     3541 gggtgacgtc ggtggagcac cgggaggcat cgacgagtgc cccctcaagt ccgagtacca
     3601 actgcgttga gcacgtgaga tatccgagca tcgagccgtg cggctcgatg ctcgagcgtg
     3661 tgttccccaa ctccccagcc cgtatcaacc gaccggtggc atgaacgttc ggttgggcag
     3721 cagctggcga gccgagtatg tgtcgatctg ctgcgtgcgc cccgacgcag accaatcttc
     3781 gacgccaccg agaaagttga aataacggaa gctgagctcc caggcgtctt cgtgcgtgcg
     3841 gagcactggg tccgtgcaga gctgctcgaa gagctgcgca ccatcctctg gccgataggt
     3901 cgtccacatg gcgagcacgc gccgttcctg ctcgaagaag cgacgagacg ccacccagcc
     3961 cccgaacact cccagcacat tgcgcaaagt ccaagccaag tcgagcccgt agggcaactt
     4021 cacgttttcc acgcggactg ccgcgttgag caaattgacg ttttctcggg cctgggagac
     4081 gtgggccaga tcgctcaccg ggtgaaacac gtgcgcggtg tgcttcggat ccaagacgac
     4141 gagcgcccgg tgccgctcca tgttcatggc cagccagaac ctgtgcgttt tactccaagg
     4201 gaaatgcgat tcgctgatgt cgtcgtcaac gaacacagcg ctgggagcgg ctcggaagtg
     4261 cttggcggcc gcggcacgaa caatgtcact cgattgcatc gaccgtccgc ctcattttca
     4321 atctacttgc ctttaccggc gcgagagtgc ctggcttctg cgagctgccc gcgcgccggc
     4381 actcaaaaga acggcggcat gaaggttcga ttgggcagca gttggcgggc cgagtagtta
     4441 acgatctgat gcctgtttcc agccgctgac cactcttcta ccccacccgc cctgttgaag
     4501 tatcgaaagc tgagctccca gacgtcctca tgagtgtgaa gcacgggatc agtgcaacat
     4561 tgctcgaaga gctgagcacc atcccgcggc cgatatgtcg cccacatcgc cagcgacttc
     4621 ctttgttctt caagaaattt tcgagtagcc acattcccgc ccaaaggaca gatcacgttg
     4681 cgcagagccc aggccaagtc caggccgtga ggcagttcta ctccctccgc gcgaatcacc
     4741 gcatttagca ggtcgacgtt ctctcgcgct tgcgaagcga gtgcgagatc ggtcaccgga
     4801 cgaaacacgt gtgcgacgtg agccgggtcc aaaacgatca gcaaaatgcg ccgttctgtg
     4861 ttcgtcgcaa cccagaagtg atgtttcttg caccaaggaa aatccgaatc cgtt
//
LOCUS       DASTKU010000973         7288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_636353, whole genome shotgun sequence.
ACCESSION   DASTKU010000973 DASTKU010000000
VERSION     DASTKU010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636353"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFQ61_34545"
     CDS             <1..275
                     /locus_tag="VFQ61_34545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959680.1"
                     /translation="TFDMSLDRVVRVTLGLLVAACHHDSRPAEGPMERAGRGVDRAAE
                     KTGAALDKAAKATGKALGKAAHETGAAFERAGQKLEGEDPGPTHSK"
     gene            complement(300..1955)
                     /locus_tag="VFQ61_34550"
     CDS             complement(300..1955)
                     /locus_tag="VFQ61_34550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9959681.1"
                     /translation="MSSTLTSEADRSRADGPSVRRPSIPVRLGSLPADSEEDLRFVQD
                     RLALFGKTTFLISGMFLLVTSTADLVAAVPRYSQLARVCHVVGTLSALALWRIAASRR
                     PLSPVALHGLDASAMLGICWTFAGLGHFGLQPYGAYTAMLAITHVSVSRAIVVPSLPR
                     RTFWLGLFGFASLVVSRAVGPLGADLAALPGARARVVIEAVLWSTAGTAVSTLASAVI
                     YGLQKKALQARQLGQYTLEEKIGEGGMGQVFRARHAMLRRPTAIKLLSSDGSEEQLRR
                     FEKEVRLTARLTHPNTISIYDYGRTPSGIFYYAMELLDGLTLEDLVAQYGPQPPSRVI
                     HLLLQVCGALREAHGSGLIHRDIKPANVFLCRRGDLPDFVKVLDFGLVRQLNGDGDST
                     RSSVNAVVGSPMYLSPEAIITPARLDARADIYGLGCLAYYLATGEPPFQGRGVVELCA
                     HHLHSPPVPPSSRADVPADLERVILACLAKKPENRPDSVRAVAHALRECRDARGWDEL
                     AAEQWWATVPSVQRKPRPDAASGIALERTICCADLERRLDHSD"
     gene            complement(2041..2412)
                     /locus_tag="VFQ61_34555"
     CDS             complement(2041..2412)
                     /locus_tag="VFQ61_34555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959682.1"
                     /translation="MSPKLDGPLPIEALRDLLGICRALYAAWKESGVGPVELDELGEI
                     GRELRAALKLARTTQPNTIGHRAAWSRAEQATARLGHLVSTLEPLRPTVLAATRRLCG
                     FAASRQLSQREAKKKHARMRG"
     gene            2684..2902
                     /locus_tag="VFQ61_34560"
     CDS             2684..2902
                     /locus_tag="VFQ61_34560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959683.1"
                     /translation="MSFDVNAFLASLLVSSVGFVLLVYGRKMQRIPHVGVGLVLLVYP
                     YFVGTVLWTLLPAALLIGLLWVVVRAGY"
     gene            2961..3866
                     /locus_tag="VFQ61_34565"
     CDS             2961..3866
                     /locus_tag="VFQ61_34565"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET9959684.1"
                     /translation="MPVRFPPAEVHIDDRIVRELVLQQFPTLSALELSFCSSGWDNVT
                     YRLGPELAVRLPRIGAAVPLLLNEQRWLPRLARVVEVPVPAPVHCGVPTAAYPWPFSI
                     VPWREGVDAGVTPLLPAEAKKVGSFLRALHSADANGLGSNAWRGGPLLHRAEEVQQRI
                     GRLREIDHGTSLDYPRLLQLFDRAVEAPLDVARCWIHGDLHPKNIVSLSGKLASVIDW
                     GDMNMGDPANDLACAWLLFDPVHHTEMWRAYGVISEPTRRRAEGWAIFFGVTLLLAGL
                     SDDDVVFQVSGRRTLERLQQGASSS"
     gene            complement(3968..4897)
                     /locus_tag="VFQ61_34570"
     CDS             complement(3968..4897)
                     /locus_tag="VFQ61_34570"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9959685.1"
                     /translation="MHAAKTQRAAANMQQLSWDDLRVFVALSAGGSTRRAARELGIDA
                     TTVGRRLKALEDTLGSKLFERQPEGMRLTSAGKEVARLATELRERVQSLQQRFTGLDQ
                     RISGTVRVTAAEIMGPLVCEALREIGERYPELCIELSVTDAMLNVQHDTAELALRVAE
                     AVPEGLIGRRIAQSAVGIYASRDYVQRWGEDLRSARHTWLEWPKALQHKPAFKWVEER
                     FPERHVVLRAKSANTVLQATRAGLGVAPLACTQAFDDPRLVMLKRLPTTCSTPVWLLV
                     HPDSRDSARVRVVMDILVAALTARRRCIEGAVE"
     gene            4987..5736
                     /locus_tag="VFQ61_34575"
     CDS             4987..5736
                     /locus_tag="VFQ61_34575"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9959686.1"
                     /translation="MQDMKGKLAVITGGSTGIGLATAKALVQRSARVILFARDPKALA
                     AAQAELGPAASTVSGDVCVAADLERLFAQAREQTSSIDALFVNAGVAEFVALERADLQ
                     HYERVMDTNVKGAFLTMKHALPLLGPGSSVVFNTSVAAAIGAPRCSVYGASKGAVTAF
                     ARNLAAELLPRGVRINMLAPGPTATPILEKAPVDATGQLEMAPFVAARMRMGRLGSVE
                     EIAEAAAFLLSPRSSFIVAQELCADGGMVGI"
     gene            complement(6114..>7288)
                     /locus_tag="VFQ61_34580"
     CDS             complement(6114..>7288)
                     /locus_tag="VFQ61_34580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9959687.1"
                     /translation="EMSAAFLEIFPGNAPQGPIKILAVDDEPDVRSLLEQYFQEQIDS
                     GRYQFMFANDGQSALKVLEEHPEVHVALTDLNMPGMNGLTLLAEFERVAPALRAVVLS
                     AYGDLPNIRQAMNRGAFDFLVKPIDLGDLEITLEKAATVARQMSRAFHSLEENRTLKS
                     FIDPALLRHILPIMRSASGGLGECAEATIAVIGFPDLAKRLRQAVDEALGVGRMDVLQ
                     QTHQHLNASFDTALALLAAHDGSAVRFDGGYLIASFRGPEQLERAVRACVQIRDTLPI
                     HQATTGGVHLGSVSSGIASGTTVSGIIGAPSRQRFDHALIGGAVATAHSLFMAASPGE
                     ILLPEQLAEKLRVHFPCLPDARVQPSPSVPTACVALKPGWLPDHRASTGHEPTVNL"
BASE COUNT         1322 a         2286 c         2380 g         1300 t
ORIGIN      
        1 atacgttcga catgtcgttg gaccgagttg tccgggtcac gttgggcttg ctagtcgccg
       61 cttgccatca tgattccagg cctgccgagg ggcccatgga gcgggcaggt cggggggtcg
      121 atcgcgcggc tgagaagacc ggggccgcac tcgacaaggc cgccaaagcc acgggcaagg
      181 cgctcggcaa ggctgctcac gagacaggtg ctgccttcga gcgcgcaggc caaaagctgg
      241 aaggtgaaga cccgggcccc actcactcta agtaagggtt gctcactagg aaactcgcgt
      301 caatcgctgt ggtccagccg tcgttcgaga tcagcacagc agatcgtgcg ctccagcgcg
      361 atgccgctcg ccgcatcggg ccgaggcttt cgttggacgc tcggtacggt ggcccaccac
      421 tgctcggctg cgagctcgtc ccagccccga gcatcacgac actcgcgtaa ggcgtgggcc
      481 acggcgcgca cgctatcagg gcgattctcg ggcttcttcg ccaaacaagc caggatcact
      541 cgttctagat ccgccggcac gtccgcgcgg ctagagggcg gaaccggagg gctatgcagg
      601 tggtgcgcgc acagctctac gacgccccgc ccttggaagg ggggctcgcc ggtggccaaa
      661 tagtaagcga ggcacccgag cccgtagata tcggcccgcg catccaggcg cgcgggcgtt
      721 atgatcgctt cgggtgaaag gtacattggc gagcccacga ccgcgttgac actgctgcgc
      781 gtcgagtccc catccccgtt gagctgccgg accaggccga aatctaggac cttcacgaag
      841 tcgggcaagt ccccacgccg gcacaggaac acgttcgcag gcttgatatc ccggtgaatg
      901 aggcctgagc cgtgggcctc gcgcaaggcg ccacaaacct ggagcaacag gtgaatgaca
      961 cgactcggcg gttgaggacc atattgtgcg accaaatctt cgagcgtgag cccgtccaac
     1021 agctccatcg cgtaataaaa gatcccgctc ggggtgcggc catagtcgta gatagagatg
     1081 gtattggggt gcgtcagacg tgcggtgaga cgtacctcct tctcgaagcg gcgtagctgc
     1141 tcttcggagc cgtcgctcga caagagcttg atcgcggtgg ggcggcgcaa catggcgtga
     1201 cgggcgcgga acacctgccc catgccgccc tcaccgatct tctcttccag cgtgtactgc
     1261 ccgagttgac gcgcctggag cgccttcttc tgcaagccgt agatcacagc cgaagccaaa
     1321 gtcgagactg cggtgccagc ggtcgaccaa agcacggcct cgatcaccac acgggctctc
     1381 gccccgggga gcgcagccaa gtctgcaccc aggggcccca cggctcgaga cacgaccaga
     1441 ctcgcgaagc cgaacaatcc aagccagaac gttcggcgag gtaaactcgg caccacgatt
     1501 gctcgcgaga cgctcacgtg agtgatggcg agcatggccg tgtaggcgcc gtacggctgc
     1561 aagccaaagt gcccgagccc agcgaaggtc caacatatcc cgagcatcgc gctggcatcg
     1621 agcccatgta acgcgaccgg tgaaagcgga cgtcggctgg ccgcgatgcg ccacagcgct
     1681 aacgcgctga gcgtgccgac cacgtgacac acgcgcgcca gctgcgaata gcggggcacg
     1741 gccgcaacca agtctgcggt cgacgtgacg agcaggaaca tcccggagat gaggaaggtg
     1801 gtcttgccga acagcgccaa gcggtcctgc acgaaccgca ggtcttcctc ggagtccgcc
     1861 ggcaggcttc cgagacgcac cggaatgctc gggcggcgaa cactcgggcc gtcggctcgg
     1921 gatcggtcag cttcggaggt cagagtggag ctcacggctt ccccgtgcca tacacggccc
     1981 gggaatctcg agcaaatgac cgtcgaccgc gcagccgagc cgagttgaaa ggacgcgact
     2041 ttagccgcgc atgcgagcgt gctttttctt cgcctcacgc tggctgagct ggcggctcgc
     2101 cgcgaagccg cagaggcggc gggtagcagc gagcacggtg ggcctgagcg gctccaaagt
     2161 gctcaccaga tgccccaggc gtgcggttgc ttgctcggct cgagaccagg ctgcccgatg
     2221 accaatggta ttgggttgcg tggtgcgcgc caatttcagt gccgcgcgca attcacgccc
     2281 aatctccccc agctcatcca attctaccgg gcccacgccg ctctctttcc aagccgcgta
     2341 aagcgcgcgg cagatcccga gcaaatcgcg aagtgcttcg atcggtaaag ggccgtcgag
     2401 cttcggagac acccgcgtcc attacgagct tcgggcgcga acgtcaatgc cagctcggac
     2461 atgcgcgtgc gttggggcaa cgtcgccggg tgacgaaccc cgacaattca ctctctgggg
     2521 tgattattgg tgcagaacgg cgagggatgg acgctcgtcg agagcccgca cgtcgacgaa
     2581 agccgtcgcg ccaaacaatc cagtccgggc aaatttcgga acggcagagc aaggggagtt
     2641 tgcgcacggg ttcgaacgaa gttcgagtag gttggctgac gcgatgtcgt tcgatgtcaa
     2701 tgcgtttctg gcgagcttgc tcgtctcatc cgtcggcttc gtactgctgg tttacggtcg
     2761 caagatgcaa cgcatcccac acgtgggcgt ggggctcgtc ttgttggttt atccctactt
     2821 cgtggggact gtgctctgga ccttgctccc ggccgcgctg ttgattgggt tgttgtgggt
     2881 cgtcgtgcgg gccgggtact gaatctgctt ggtttcgttg gccaatccgt cgatgctcgc
     2941 aaacgcccga ggagagccca atgcccgtcc gcttcccgcc cgccgaggtc catatcgacg
     3001 atcgaatcgt gcgcgagctc gtgctccagc aatttccgac gctgagcgcg ctcgagcttt
     3061 ccttctgctc gagtggctgg gacaatgtga cgtatcggct tggcccggag cttgccgtgc
     3121 gcctgccgcg cattggcgcg gccgtcccac tgttgctgaa tgagcaacgt tggctcccga
     3181 gactggcccg cgtcgtcgag gtgcccgtac ccgcgccggt tcattgcggc gtaccgacgg
     3241 cggcgtatcc ttggccgttc agcatcgtgc cgtggcgcga aggggtagac gccggcgtaa
     3301 ctccgctatt gccggcggaa gcgaagaagg tcggcagctt tctgagagca cttcacagtg
     3361 ccgacgcgaa cggcctcggt tctaacgcct ggcgcggtgg cccgctgctc catcgcgccg
     3421 aggaagtgca acaacggatt ggccgtttgc gcgagatcga ccatggcacg agcctcgact
     3481 atccgcgtct tctacagctt ttcgatcgcg ccgtcgaagc gcccttggat gtcgcacgct
     3541 gttggattca tggcgacttg caccccaaga acatcgtgtc tctctcgggt aagttggcgt
     3601 ccgtcatcga ctggggcgac atgaacatgg gcgatcccgc taacgacctg gcgtgcgcgt
     3661 ggctgctctt cgaccccgtc catcacacgg aaatgtggcg tgcctatggc gtaatctccg
     3721 aacccaccag gcgccgcgcc gaaggctggg ccatcttctt cggcgttacc ctacttctcg
     3781 ccgggctcag cgacgacgac gtcgtgttcc aggtttcggg tagacgcaca ttggagcggc
     3841 tgcagcaagg tgcttcgagc tcatgagtga cgtcctaatg caagcacacc tgttctcggt
     3901 cagctatcga gtgagtagcc gtccatccgc tccagctcgc caatgagggc cgcgagcgtg
     3961 gcattcgtta ctcaacagct ccttcaatgc agcgacggcg ggcggtcagc gcggcgacga
     4021 ggatgtccat gaccacgcgg acgcgagcgg agtcgcgaga gtccgggtgc acgagtagcc
     4081 agacgggggt cgagcaagtg gtcggcaagc gcttcaacat caccaaacgt gggtcgtcga
     4141 aggcttgcgt gcacgcgagc ggcgcgactc cgagtccggc gcgggtggct tgaagcacgg
     4201 tgttcgcgct cttggcgcgt aacacgacat ggcgctcggg aaagcgctcc tcgacccatt
     4261 tgaaggctgg cttgtgctgt agagctttcg gccactcgag ccaggtgtgt ctcgcagagc
     4321 gtagatcttc gccccaacgt tgcacgtaat cgcgcgaagc gtagatcccc accgcgctct
     4381 gcgcgatgcg tcgtccaatc aacccttccg gcaccgcttc ggcgacgcgc aacgcgagct
     4441 cggccgtatc gtgctgcacg ttcagcatcg cgtcggtaac gctcagttcg atgcacagct
     4501 ccgggtagcg ttcaccaatc tcccgcagcg cctcgcacac caggggcccc ataatctctg
     4561 cggcggttac gcggaccgtg cccgatatgc gctggtcgag gccggtgaag cgctgctgaa
     4621 gggactgcac acgctcgcgc agctcggtcg cgagccgcgc cacctcttta cccgcgctcg
     4681 tcagtcgcat gccctcgggc tgacgctcga acagcttcga accgagcgtg tcttcgagcg
     4741 ccttcaagcg acgcccgacg gtggtcgcgt cgataccgag ctcgcgggcg gctcggcgcg
     4801 tgctgccgcc cgctgatagc gcgacgaata cgcggagatc gtcccaggac agctgctgca
     4861 tgtttgcggc ggcccgctgc gtctttgctg cgtgcatgaa tgcgtaaaaa ccagcatacg
     4921 ttggtccagt gcttcggaca actgagcgaa gttcaggctg atgaacgggg cacgaggaga
     4981 gaacgtatgc aagacatgaa aggcaagctc gcggtcatca ccggaggcag caccggcatt
     5041 ggtctggcga cggcgaaggc gctcgtccaa cggagcgctc gtgtgatcct gttcgcgcgt
     5101 gatcccaagg cgctggccgc cgcccaggcc gagctcgggc ctgcggcctc gacggtaagc
     5161 ggagacgtgt gcgtggccgc cgatctcgag cggctgttcg cgcaggcgcg tgagcagacc
     5221 tcgagtatcg atgccctgtt cgtcaatgct ggagttgccg agttcgtggc gctcgaacga
     5281 gccgacctcc agcactacga acgtgtcatg gatacgaatg tgaagggcgc gtttttgacg
     5341 atgaagcacg ccctgccgtt gctcggcccg ggcagcagcg tcgtcttcaa taccagcgtc
     5401 gcggcggcca tcggtgctcc gcgctgtagt gtgtatggcg cgagcaaggg agcggtcact
     5461 gcgttcgcgc gaaacctcgc ggccgagctt ctgccacggg gagtgcgcat caacatgctc
     5521 gcgcctgggc ctaccgcgac tccaattctc gagaaggctc cggtggacgc tacggggcag
     5581 ctggagatgg caccgttcgt ggccgctcgc atgcgcatgg ggcgtctagg gagcgtcgaa
     5641 gagatcgcag aggcggccgc gttcctgctg tcgccccgat cgtccttcat cgttgcgcag
     5701 gagctatgcg cggacggcgg gatggtcgga atctgatgcg ttctcctgcg gagccgcgtg
     5761 gcggggacgc gatgcaaagt tgcgggtgac tgggagaggg tggccacgct cgctcaatcg
     5821 ccggacccca actcctggcg aggagcgcgt cgtggtgaac gacgcggtga tcgcgcagct
     5881 cggagggacg gccccctcgg aagttctgaa gagcttcggc atcgagctga tcaccaaatt
     5941 cggcgagcgc tgagagccgc gcgttatagg gtggggcctg cgcggtgagc acggcgaccc
     6001 cgctagtgga attgtctgcg ctcacgacga tgaaagtcgc cgagtcgcgc ccgtatgcta
     6061 gtcgggccct cgacaagagc tcgactacgg ggtccagtgg ccgagcagat cggtcagagg
     6121 tttacggtgg gttcgtgacc cgtactcgcg cgatggtccg gcaaccaccc gggcttgagg
     6181 gccacgcaag ccgtcggcac actgggcgag ggttgaacgc gcgcgtccgg cagacacggg
     6241 aagtgaaccc ggagcttctc cgccagctgc tcgggcaaca gaatttcgcc tggtgacgcg
     6301 gccatgaaga ggctgtgggc ggtcgcgacc gcgccgccga tcaaggcgtg atcgaagcgc
     6361 tgccgactcg gcgcgccgat gataccggat accgtggtcc ctgaagcaat tccggaagaa
     6421 acgctgccga ggtgcacccc gcccgtcgtg gcttgatgga tcgggagagt atcgcggatc
     6481 tggacgcacg cgcgcaccgc gcgctcgagc tgttctggtc ctcgaaagct agcgatcaga
     6541 tagcccccgt cgaaacgcac cgcgctgccg tcgtgcgccg ccaacaaggc cagcgccgta
     6601 tcgaagctgg cgttcagatg ttggtgggtc tgttgcaaga cgtccatccg cccgaccccg
     6661 agcgcttcat ccactgcctg tcgcagccgc ttcgccaaat caggaaagcc gatcaccgcg
     6721 atagtagcct ctgcgcactc gcccagccca ccggaagccg agcgcatgat gggcaaaatg
     6781 tggcggagca gggccgggtc gatgaaagac ttgagggtgc ggttttcctc caaggagtga
     6841 aacgcgcgag acatctggcg cgcgaccgtg gctgccttct ccagcgtgat ttccaaatca
     6901 cccaggtcga tgggcttgac caggaagtcg aaggcgcccc ggttcatggc ctgtcgaata
     6961 ttgggtaaat cgccgtaggc tgatagcacc accgcccgca acgctggtgc gactcgctcg
     7021 aactccgcga gcaaggtgag cccattcatc cctggcatgt tgagatcggt caacgccacg
     7081 tgaacctcgg ggtgctcttc caagaccttg agcgcggact gcccgtcgtt tgcgaacatg
     7141 aattggtagc gcccgctgtc gatctgctct tggaaatact gctccagcaa ggagcgcaca
     7201 tctggctcgt cgtccaccgc cagaatcttg atcggaccct gcggtgcgtt gcccgggaaa
     7261 atctccagga aggcggcgct catttcgc
//
LOCUS       DASTKU010000974         8200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_636628, whole genome shotgun sequence.
ACCESSION   DASTKU010000974 DASTKU010000000
VERSION     DASTKU010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636628"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VFQ61_34585"
     CDS             complement(<1..969)
                     /locus_tag="VFQ61_34585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HET9959688.1"
                     /translation="MKFLCPSCKAKYQIADEKVSGRSVRMKCRKCGYLITVSSADAES
                     WPSTPPSAPEPALTGASPAAVHPVLEKPVPERAPEKPPANRPAPPEKSRPLGARPGGP
                     SAGGPSAGGHNPAAAGNTVAGAGGALSPGTAGAPRPVQRSALSIPRPGASGPASSTGT
                     PSTDRRLPSQLRTQVGGLGPSAAAPAKPAAHGTAPAHSPAAPAVHHSSGATNAQPAAQ
                     KAAPAGTPNGPPARGVASFLDAEFEEEPTRIAAPLNALASALSSSAGTPAVQKPAPAA
                     APTTTTTASHLVTDPLNLPGDEWFVGINGVPVGPIRLAELRTKAATG"
     gene            complement(1147..2631)
                     /locus_tag="VFQ61_34590"
     CDS             complement(1147..2631)
                     /locus_tag="VFQ61_34590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9959689.1"
                     /translation="MNGKKPIGKILLQQRALRPEELERALSEPAPGRLASRLAATGVV
                     SDVAALKALSEQHGIPGIDLSQLCLSLEQLDLLPREIAEKHRILPVLQREDRLFVAMA
                     DPREKSVIDELEFVTGKRVYPYVALESAISQVIDDAYQRKAAGHAYYIGPNCPAELLQ
                     KVGINSDGEVVSDGTPSVLPQPEADSMRPNASLPHPSQGVVVDDQVDQVSRADDVEEQ
                     DFGKWSDVTLGGASERAPAGSKGKHVLVVDDEAEIRKMLTHLLTRAGYRVIEAERGMQ
                     ALRIVREQSPDLIVLDAMLPEVHGFEIARRIKSSELYRHIPIIMVSAVYRGWRYAEDL
                     KNSFGVDRFIEKPFRIGDVLESVEAALKERTQTSSDAERTLSAAAEQALNQGIEAYRA
                     GRIDEAIEHLKRGTAIDPLAFRLHFHLGLLYGKKGQIFDAIAELEAAVESYGEHFPAL
                     KNLAILYQKAGFRNRAVEMWERALPLSPDEPTRQSIRQHLLTLL"
     gene            complement(2873..4012)
                     /gene="tgt"
                     /locus_tag="VFQ61_34595"
     CDS             complement(2873..4012)
                     /gene="tgt"
                     /locus_tag="VFQ61_34595"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET9959690.1"
                     /translation="MDSSTEGVISFQLEATSGHARAGRLVTRHGVVETPAFQPVATRA
                     TVKALAAEDVWSTGARLVIMNTYHLWLRPGAELIAEQGGLHRFARWSGSITTDSGGFQ
                     AFSLAERVKVSEQGFEFSSHLDGRRLKLGPEEAMRIQGLLGSDIAMQLDICAPGTAAR
                     AELVLAVERTTRWAERCLAARTPGQAVFGIIQGGTDLELRLRHAEELARLPFDGLALG
                     GFSVGEPIADMYRVLERVGPAVDPRRPHYLMGVGTPEDLIRAIGFGIDMFDCVLPSRN
                     ARNGQAFVSTGKLVIKNARYQSDPRPLEEGCGCAACSAGYSRAYLRHLYLANEISAHR
                     LITLHNIWHYQRLVADARRAIVEGRYSEWAADKLAALTTPAPSPN"
     gene            complement(3988..5127)
                     /gene="queA"
                     /locus_tag="VFQ61_34600"
     CDS             complement(3988..5127)
                     /gene="queA"
                     /locus_tag="VFQ61_34600"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET9959691.1"
                     /translation="MAVRKPHERRADRAIPGLFRKFELYAARVVKAELFEYSLPDERI
                     AKHPPDLRDGGRMLVLEGERITHASVRELPSWVAAGALFVFNDTRVRRARIFGVRPSG
                     GRVEFLMLGPSRAREGTRERWRALGRASKALLPGARVRALDLAIEVIGKEPDNGVLDL
                     QIESAEPVEVVLERAGEVPIPPYLGRDAEALDLERYQTVYARRTASVAAPTAGLHFTE
                     ALLEALGDRGVCVARLELEVGIGTFRPVTADDLDQHPMHEEHFVIGPELVQAIQEARE
                     REAPVVAVGTTVVRALESARDPARPGHVLAGARSTRLLIQPGYSFSVVDGLFTNFHQP
                     RSTLLALVAAFAGYSETMAAYRAALGADYRFLSYGDAMWIPQRKA"
     gene            complement(5183..6544)
                     /locus_tag="VFQ61_34605"
     CDS             complement(5183..6544)
                     /locus_tag="VFQ61_34605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9959692.1"
                     /translation="MGKILVVDDQRNMRTTLAMMLRGQGYEVDEAEDGLQGKEIGATG
                     AYDIVITDLRMGEYNGIDVLKRVKDAQPMTEVIIMTAYGTIESAVEAMRVGAFDYIQK
                     PFSEQELIVKVSKALAIRRLQGQVQLFAQEFRDRYQLENVIGRSQGIRDVLSRVIKIA
                     PTDSTVLITGESGTGKELVARAIHANSKRAQRPFVTVNCAAISETLLESELFGHAKGA
                     FTGAVAARKGLFEEADGGTFFFDEIAETTPVFQAKLLRAVQEGEVRRIGENKPIRVDI
                     RVVAATNVDLSRAVEERRFRQDLYYRLNVARFQLPPLRERSEDIPLLVEHFLNKYSRK
                     LNTRVRLAEGVLEALMAYDYPGNVRELENLVEQSVALAVNGVIRVDDILPRPPKSEEK
                     PQGRTLADIVDDAERDAIEAALRAFDGNRERAAVSLDISLTTLWRKMNRLRITADDRR
                     AAP"
     gene            6884..7333
                     /locus_tag="VFQ61_34610"
     CDS             6884..7333
                     /locus_tag="VFQ61_34610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959693.1"
                     /translation="MASLSEQLGAGSKREAVIDDALKVLDEEVADKGGLSGMAIKGAY
                     KLVQGVRPGFLRQVVNSLLGDFLLALDPVYQEAKSKGRPAGAYLQENSGRVADALLVV
                     TDRKAQRADSAVVKGAYDKLRPMAKKQVEAAAPRLARLLEKHAEPTA"
     gene            7501..>8200
                     /locus_tag="VFQ61_34615"
     CDS             7501..>8200
                     /locus_tag="VFQ61_34615"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HET9959694.1"
                     /translation="MTSVPTASGQQTQLRGAVVRFAGDSGDGMQVTGDQFTVAAALAR
                     NDLATFPDFPAEIRAPAGTTYGVSGFQIHFASEHVYTPGDAPDVLVAMNPAALKTNLK
                     DLKRGGLLIVNTGAFNSANLKKAGYEVSPLENDSLEGYQLLSIDITKQTQNAVKEAGL
                     SNKDANRCKNFWTLGLMFWIYGRPMEPTLRWIQQKFGKRPEVATANELALKAGHAYGE
                     TAEIYQYRYEVPAAP"
BASE COUNT         1516 a         2667 c         2509 g         1508 t
ORIGIN      
        1 gccggtcgcg gccttggtgc gtaactcagc caagcgaatt gggccgacag gcacgccgtt
       61 gatcccgacg aaccattcgt cgccaggcag attcagcgga tccgtaacga gatgactggc
      121 ggtcgtcgtg gtcgtcggcg cggcggctgg tgcaggcttt tgaaccgccg gcgttcccgc
      181 ggatgagctc aaggcgctcg ccagcgcgtt gagcggagcc gcgatgcgcg tgggctcctc
      241 ttcgaactca gcgtctaaaa aggaggctac cccgcgagcg ggaggcccgt tcggtgtgcc
      301 cgccggggcg gccttctgtg ccgccggttg cgcgttcgtg gcgccgctcg agtgatgcac
      361 agctggtgca gctggcgaat gtgcaggcgc agtcccatgc gcggccggct tggctggcgc
      421 ggccgcggaa ggcccaaggc cgccaacttg cgtcctgagc tgcgacggca aacgacggtc
      481 cgtagatggt gttccggtgc tggaagccgg tccgctcgcg ccgggtcgag gaatacttag
      541 tgccgagcgc tgtaccgggc gcggcgcgcc cgcagtacct ggtgaaagcg cgcccccagc
      601 accagcaacg gtattgcccg ctgcggcggg gttgtgccct ccggcactgg gtccaccggc
      661 actgggtcca ccaggacggg ctccaagcgg acgcgatttc tccggaggcg cgggtcgatt
      721 ggcaggcggt ttctccggtg ctctttccgg cacgggcttc tccagcactg gatgaacggc
      781 cgctggtgaa gctcccgtta gcgccggttc gggcgcagaa ggcggcgtcg aaggccagga
      841 ctctgcgtct gcgctcgaga cggtgatcag gtagccgcac ttgcggcact tcatccgcac
      901 cgaacgaccg gagaccttct cgtcggcaat ctgatatttg gctttgcagc ttgggcagag
      961 gaacttcatt cagccagatg ccgtcgaggt cagcgtccca aaggcttgag gatagcggag
     1021 gctagcatca ctacgtcact tttcagcagc ccgaaagcgc agctcggccg gggagcccgg
     1081 cggctcgccg ggtgcccgga atctttgtga aagttgcgag cagtctgcgg ctcttaccat
     1141 gggccactag agcagcgtca gtaggtgctg gcggattgat tggcgcgtcg gttcatcggg
     1201 agacagcggc aaagcccgct cccacatttc cacggctcga ttccgaaatc ctgccttttg
     1261 gtagagaatc gccaagttct tgagagccgg gaaatgttcg ccatagcttt ccactgccgc
     1321 ctctagctcc gcgatcgcgt caaatatctg ccctttcttt ccgtagagca gccccaggtg
     1381 gaaatggagc cgaaaagcta gcggatcgat tgcggtgccg cgcttcaggt gctcgatggc
     1441 ctcgtcgatg cgcccggcgc ggtatgcctc gatgccctga ttgagcgctt gctcggccgc
     1501 cgcggacaag gttcgctctg catccgagga ggtctgggtg cgctctttga gcgctgcctc
     1561 cacgctctcg agcacatcgc cgatgcgaaa tggcttctcg atgaagcgat cgactccgaa
     1621 tgagttcttg agatcttctg cgtaacgcca gccccggtac acagcgctga ccatgatgat
     1681 cggaatgtga cggtagagct cgctgctctt gatgcggcgg gcgatctcga agccgtgaac
     1741 ctccggaagc atcgcgtcca acacgatcag atcgggcgac tgttcccgga cgattcgcaa
     1801 ggcctgcatg cctcgttcgg cttcaatcac ccgatacccg gcacgtgtca gcagatgcgt
     1861 cagcatcttg cggatctcag cctcatcgtc gaccacgagg acgtgcttgc ccttgctccc
     1921 ggctggtgca cgttcggagg cgcccccgag cgtgacatcg gaccactttc cgaagtcctg
     1981 ctcttctacg tcgtccgcgc gcgaaacttg gtccacctgg tcgtctacga ctacgccctg
     2041 cgacgggtgc ggaagcgagg cgtttggacg catcgaatcc gcctcgggct gcggcagcac
     2101 cgaaggtgtg ccatcggaca cgacttcacc gtccgagttg atgccgacct tttgcagcaa
     2161 ctccgcgggg caattcgggc caatgtaata agcgtggccg gccgcctttc gctgataggc
     2221 gtcgtcaatg acttgcgaaa tcgcggactc gagcgccacg tacggataga cgcgcttacc
     2281 ggtgacgaac tcgagttcgt caatcacgct cttctcgcgc ggatctgcca tcgcaacgaa
     2341 taggcggtcc tcgcgctgga gcaccggcaa aatccggtgc ttttccgcga tctcgcgcgg
     2401 cagcaaatca agctgttcca ggctcaaaca cagctgcgat aggtctatgc ctggaatccc
     2461 gtgttgctcg gacagcgcct tcaacgcggc gacatccgac acaactccag ttgccgcgag
     2521 acgcgacgcg agtcgtcccg gtgctggctc cgagagcgcg cgttccagct cctccggccg
     2581 aagcgctcgc tgctgcagca aaatcttgcc gatgggcttc ttgccgttca tcgcgcctgc
     2641 cgactcgcat catgacacgg cccgagcgtc agggtccatt ctctcgcaaa ctgttgctct
     2701 cggaacccct gactcgaaat cccacatgac gctgcccgta tcccgaagac cacgcgggct
     2761 tccagcgcct gaaaaccaca atggctcccg caccgccaaa ccagagcagc aaacattcac
     2821 cgggcgcgac tgaacccccc cacgcgctta cggcccggaa cccggaaagc gcttagttcg
     2881 gggaaggtgc cggcgtagtc aaagcggcca gtttgtcggc ggcccactcc gagtagcgcc
     2941 cttcgacgat ggcgcgccgg gcgtcagcta ccaaccgctg gtagtgccaa atgttgtgca
     3001 gtgtgatcag tcggtgggcc gagatctcgt tggccaaata gaggtgccgc agataagccc
     3061 gtgagtaccc cgccgaacat gcggcacatc cgcagccttc ttcgagtggg cgcgggtcac
     3121 tttggtagcg cgcgttcttt atcaccagct tacccgtgga aacgaaggct tgaccattcc
     3181 gagcgttgcg tgagggtaaa acacaatcga acatgtcgat tccgaagcca attgctcgga
     3241 tcaagtcctc gggtgtgccg acgcccatca agtaatgggg gcgccgcgga tcgacagcgg
     3301 gtccgacccg ttcgagcacg cgatacatgt ccgcgattgg ctctccgacg ctgaaccctc
     3361 cgagggccag accatcgaac ggcagacgcg ccagctcctc tgcatgtcgc aggcgcagct
     3421 cgagatcggt gccaccttga ataatgccga acacggcctg acccggcgtg cgcgcggcga
     3481 gacaacgctc cgcccagcgc gtggtccttt cgaccgcgag cacgagttcg gcacgagccg
     3541 ccgtgccagg ggcgcagata tctagctgca tggcgatgtc agagccgagc aggccttgga
     3601 ttcgcatggc ctcctcgggg ccgagcttca aacgacgccc gtccaaatgc gacgagaact
     3661 cgaagccttg ttcgctaacc ttcacgcgct cagctaggga aaaagcctga aagccgcccg
     3721 agtcagtcgt gatcgagcca gaccaacggg caaagcgatg cagcccgcct tgttccgcga
     3781 tcaactccgc tcccggcctc aaccagaggt ggtaggtgtt catgatcacg agccgtgcgc
     3841 ccgttgacca gacatcctca gctgcaagcg cctttacggt cgcccgcgtc gctactggtt
     3901 ggaacgcggg tgtctcgacc acgccgtggc gcgtgacgag gcgaccggcc cgtgcgtgcc
     3961 ccgacgtggc ctcgagctgg aacgaaatca cgccttccgt tgaggaatcc acatcgcatc
     4021 tccataggac aagaatcggt aatccgcgcc cagtgcggct cgatatgcag ccatggtctc
     4081 ggagtagccc gcgaacgccg cgaccagcgc gagcaaggtg ctacgcggct ggtgaaagtt
     4141 cgtgaatagc ccgtccacca cggagaacga gtacccgggc tgaatgagca gccgggtgga
     4201 gcgcgcgccc gcgagtacgt gccccggtcg cgctggatct cgcgcgctct cgagcgcacg
     4261 cactacggtc gtgccgacgg ctaccacggg agcctcgcgt tctcgcgctt cctgaatggc
     4321 ctggaccaac tcgggcccaa tcacgaagtg ctcttcgtgc atcgggtgct gatccaaatc
     4381 gtccgccgtt accggtcgaa atgtgccgat tcccacctcg agttcgagac gcgccacgca
     4441 gacgccccga tcgccgagcg cttcgagcag ggcctccgtg aagtgcaaac ctgcagtcgg
     4501 cgcggccacg ctggcggtgc gcctcgcgta taccgtttga tatcgctcga gatcgagcgc
     4561 ttcggcgtcg cgtcccaagt acggagggat cggcacttca ccggcacgtt ccaacaccac
     4621 ttccaccggc tccgcggact cgatctgcag atcgagtacg ccattatcgg gctccttgcc
     4681 gatcacctcg atcgcgagat ccagggctcg aacacgcgcg ccgggcagca gcgctttgct
     4741 cgctcgcccc aacgcacgcc agcgttcgcg ggtgccctcc cgagcgcggc tcggccccaa
     4801 catcaagaac tcgacccgcc cacccgaagg gcgaacgccg aagatgcggg ctcgccgcac
     4861 gcgcgtgtcg ttgaatacga acagcgcccc ggccgcgacc cagctcggga gctcccgtac
     4921 tgaagcgtgc gtgatgcgct ccccttcgag caccaacatg cgcccgccgt cacgcaagtc
     4981 cggtggatgt tttgcgatac gctcgtctgg taaggagtac tcgaacagct cggctttcac
     5041 gacgcgcgca gcgtatagct cgaatttacg gaagagcccg ggaattgctc gatctgcccg
     5101 tcgttcgtgt ggctttcgaa cggccatgga aacggcaagc aggcacgcgc ggcgctgctt
     5161 ggtggcgtgg gtctgggccg cgctaaggcg ccgcgcggcg gtcgtccgcc gtaatgcgga
     5221 gacggttcat cttacgccac agcgtggtca gactgatgtc gagcgacacg gccgcgcgct
     5281 cgcggttgcc gtcgaaggca cgtaacgccg cctcgatggc atcgcgctcc gcgtcatcca
     5341 cgatatcggc caaagtgcgg ccctgcggct tctcttcgga ttttggcgga cgcggcagaa
     5401 tgtcgtccac tcggatcacg ccgttcaccg cgagcgccac cgattgctcg accaagtttt
     5461 cgagctcgcg cacgttgccc ggatagtcgt aagccatcag cgcctccagc acaccctccg
     5521 ccaagcgcac gcgagtgttc agcttgcggc tgtacttgtt gaggaagtgt tcgaccagta
     5581 gcgggatgtc ctcgctgcgc tcacgcagcg ggggaagctg aaagcgcgcc acgttgagcc
     5641 ggtagtagag gtcctgtcgg aagcgccgct cctcgaccgc gcgcgacaag tcgacattgg
     5701 tggccgccac cacccgaatg tccacacgaa tcggtttatt ttccccgatt cgacgcacct
     5761 ctccttcttg cacggcgcgc aacaacttgg cctgaaacac cggcgtcgtc tcggcgatct
     5821 cgtcgaagaa gaacgtcccc ccgtccgcct cttcgaacaa ccctttgcgc gcggcgactg
     5881 cgccggtgaa ggcgcccttc gcatgaccga agagctcgct ctccagcaac gtctcgctaa
     5941 tcgcggcgca gttcacggtg acgaacggac gctgcgcacg cttgctgttg gcgtgaatcg
     6001 cccgggcgac gagctcctta cccgtcccgc tctcgcccgt gatcagcacc gtcgaatccg
     6061 tcggcgcaat cttgatcacg cgtgacaaga cgtcccgaat gccttgggag cgcccgatca
     6121 cgttttcgag ctggtatcga tcacgaaact cctgtgcgaa cagctgaacc tgcccctgca
     6181 agcggcgaat cgcgagcgcc ttcgagacct tgacgatcag ctcctgctcg ctgaagggtt
     6241 tctgaatgta atcgaaggca ccgacgcgca tggcttcgac cgcactctcg atcgtgccgt
     6301 aggcggtcat gatgatcacc tccgtcatcg gttgcgcgtc cttcacgcgc ttgagcacgt
     6361 cgataccgtt gtactcgccc atacgcaggt cggtgatgac gatgtcgtac gcacccgtcg
     6421 cgccgatctc tttaccctgc aggccatctt cggcctcgtc gacttcgtac ccttgccccc
     6481 gcagcatcat ggctagggtg gtgcgcatat tccgttgatc gtcgacgacg agtatcttgc
     6541 ccatgtttcg cgtctcgccg gcgaggctgg ccgggagctc actcgcttac catgctcgaa
     6601 ggcacaaagc cgtcaacggc ggcgttcttt cgagccggtt cccggccgca agcgccgccc
     6661 gccggggctg ggtcgggcgc ggcgaggcca tcattcaggt tctagagcct actttcgtgc
     6721 tcacgcattt cgctccgccg gcagtcggtg ctaagagccg ccaccatccg caagaacacc
     6781 gcgaggccgc gagcgtttgg tgaggccagt cgaatcgccg aacccactcg tcgtcagcct
     6841 cgcgccggtt ctttcgccga gatgcgtagg agaaggaagc gacatggcaa gtttaagcga
     6901 acaactgggc gccggcagca aacgtgaagc ggtgatcgac gacgccctca aagtgttgga
     6961 cgaagaggtg gcggacaagg gcgggctgtc cggaatggcc atcaaaggcg catacaagct
     7021 ggtgcagggc gtgcgcccgg gcttcttgcg tcaggtcgtg aacagcttgc tcggcgactt
     7081 cttacttgcc ctcgatccgg tctaccaaga agccaagagc aaaggccgcc cggcgggagc
     7141 ttacctacaa gaaaactcgg gacgggtggc cgacgccctg ctcgtcgtca cggatcgcaa
     7201 agctcaacga gcggacagcg ccgtggtcaa aggcgcctac gacaagttgc gcccgatggc
     7261 gaagaagcag gtcgaggcgg cggcaccgcg gctcgcgcgg ttgttggaaa agcacgccga
     7321 gcccacggcg tgatttggcg agagctcagc gcaacgctcg aagcatttcg acaacgtttc
     7381 gaaaaccttc ctatttttct ggctgatttc taagtggctg gcagccggaa ttcgcgtttc
     7441 cccatcgggt agcttgacaa tggtcggcta aacccaaaac ttccgcaaag tttcgtgact
     7501 atgacctccg ttcccaccgc gtcgggccag caaactcaac ttcggggagc cgtcgtgcgg
     7561 tttgccggtg actccggcga cggcatgcaa gtgacaggcg atcaattcac ggtcgcggcc
     7621 gctctcgcgc gcaacgatct cgcgacgttc ccggattttc cggcggaaat ccgggctccc
     7681 gcgggcacca cctacggagt atccggcttt cagatccact tcgcctccga acatgtctac
     7741 acgccaggcg acgcgccgga cgtgctggtc gcgatgaacc ccgccgcgct caagaccaat
     7801 ctgaaggatt tgaagcgcgg cggtctcttg atcgtgaaca ccggcgcatt caactccgct
     7861 aatttgaaga aggcgggcta cgaagtctct ccgctcgaaa acgacagtct cgagggctat
     7921 cagctgcttt cgatcgacat caccaagcag acgcagaacg ctgtcaagga ggcggggctc
     7981 tcgaacaagg acgcgaaccg ctgcaagaac ttctggaccc tcggtctgat gttctggata
     8041 tacggacggc caatggagcc gactctgcgc tggattcagc agaagttcgg taagcggccg
     8101 gaggtggcga cggctaacga gctcgcgctg aaagccggcc acgcctacgg cgaaaccgca
     8161 gagatctacc agtaccgcta cgaagtcccg gccgcccccg
//
LOCUS       DASTKU010000975         5701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_636698, whole genome shotgun sequence.
ACCESSION   DASTKU010000975 DASTKU010000000
VERSION     DASTKU010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636698"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1294)
                     /locus_tag="VFQ61_34620"
     CDS             complement(200..1294)
                     /locus_tag="VFQ61_34620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959695.1"
                     /translation="MGRLALRRALLVVVAGAALTAVLAGLGRLGISFGWGPKYALAHG
                     PLFVVGVFGTVIALERAVALGRPIALLAPAVAAASAMGLLLGRPESAWLAVLAAVLLV
                     LVNGAIVRRQSAGFTWLMLLGTLLLGAGNLAWACGKPIAAAAPAWLVFFVATISAERL
                     ELSRLARAPSWASQALLSLALLSTLLACASALGIPNATRMLGPPLALIGAWQLRFDIA
                     RRTVRAAGLPRFSAVGIMSGSLWLCVAGVLLMIVGLPQFGVLYDAVLHAVFVGYALSM
                     VFAHAPIILPAVVGIRVPFSPALYAPLVVLHAGLLARLIGDLGENADLRRLGSIVNAL
                     ALAVFVLAMSYSRGSARSTHHLVNREAHKL"
     gene            complement(1282..1599)
                     /locus_tag="VFQ61_34625"
     CDS             complement(1282..1599)
                     /locus_tag="VFQ61_34625"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET9959696.1"
                     /translation="MTDQSSLRTQLMTALRAVLDPELGINIVDLGLVYEADVDAQGHA
                     TVKLTMTSPACPLGEHVRVEAEQQLLAIRGLESVQVELVWEPLWSPDRMSLAAKELLG
                     WAG"
     gene            complement(1833..2321)
                     /locus_tag="VFQ61_34630"
     CDS             complement(1833..2321)
                     /locus_tag="VFQ61_34630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959697.1"
                     /translation="MIGSFLRVIRRIMQSIIPQDVRIEQVLTALQHAYSVAGPIGQLE
                     LRSAILEISSRVWAMFEQCASHETEADTVMASECLLIQEQLCQSVGALRAPARHSLSA
                     SGTRGRQSVGSLRAPELAPSTGVPGDSLDEATRAKVVGQVIKIVDNILSDRRLTDISA
                     LA"
     gene            complement(2544..2672)
                     /locus_tag="VFQ61_34635"
     CDS             complement(2544..2672)
                     /locus_tag="VFQ61_34635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959698.1"
                     /translation="MPDAFTCQAGDAVAWEDVCDGEPQCADAEDELLCFARRAALE"
     gene            complement(3156..4070)
                     /locus_tag="VFQ61_34640"
     CDS             complement(3156..4070)
                     /locus_tag="VFQ61_34640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959699.1"
                     /translation="MRSSIGDGGTWWRRGLMLALGASCSAACSSFDPGGPEGSGSLAF
                     TTWGEDFIEQQIPAAPSGEDGFIDGWTVRYEKFLVNFQNITVADGVGKRAAVMAGSKL
                     FDNHQPGVKPIIDFPEVSAQAWDRVSYEIAPVSASTVLDSSASEADAAEMREHGFSLL
                     VAGTASKDQRTMHFSWGFPSHTLYLDCHSSQGGREQNGVLVRSHSSVEVQLTTHGDHL
                     FYDRLQASPDPAIATSLRFEALASSDANRDGELTLAELDAQIIDVRLYDPSGLGAATY
                     GAFMRTLVRTVGHFRGEGECTVNEPAAD"
     gene            complement(4073..4924)
                     /locus_tag="VFQ61_34645"
     CDS             complement(4073..4924)
                     /locus_tag="VFQ61_34645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959700.1"
                     /translation="MTNGRVMLTRRRATWASLALVTAWCFGVGLPACNEGTTGGARVS
                     LRTRVELRPEAKLPFTTLRGWQVQLDSALIGHGPFYYFDGAPPLARSTPGFGARLARR
                     SITELLSVRVARAHPGHYASGTALGQALIPSSTDLWAGETWLAEGEGVTGNYRSASFS
                     FMAGEGPFASQLGEAVALAIGTARKPAETERHFRALAKLADIEHTAAQGVVTGCVFVP
                     AEVRGSGVITLRVDPRVWFELVDFSEIEPGSPTKLEPFPDDSQPRIAFSQGLASFSAY
                     EFSFAAD"
     gene            complement(4921..>5701)
                     /locus_tag="VFQ61_34650"
     CDS             complement(4921..>5701)
                     /locus_tag="VFQ61_34650"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9959701.1"
                     /translation="FGAQLSKRGTLDVALRPGLHALLSYGEGFRSPQARSLEDGERTP
                     FTRVVSYELGLRFRDERRLEASIAGFRTALSDDLVFDHATARNERVPGTTRTGIAAQL
                     AARPASWFLSQLSFTYAHAVFSGEAAEKSGGRYHAGELVPYAPGVVVRSDWAFTPQLG
                     SWLGAPLSGHFGSAFSYVGRRPLPYAEFGHDVFLVDASAEFKWRTFELGLEVFNLFDR
                     TFYDGEFVYASRFGGAASLVPVRHVTLGPPRSFLFTFGLSV"
BASE COUNT         1180 a         1898 c         1732 g          891 t
ORIGIN      
        1 cccccaagcg ccgaagccaa aacccaaact cgcgtccgag aacgaatcat ttccgaccgg
       61 ttgaggatat ccaagcctct cagtgagcga aatgacggtt atccacttcg cgcttcgcac
      121 tccgcgtaaa tcagcctgta attcgcgccg acgacagacg aagtcgacag aggcggagcg
      181 agctcggtgc tctacacctt catagcttgt gcgcctcgcg gttcaccagg tggtgagtcg
      241 atcgtgcgga tccgcgcgag tacgacatgg caagcacgaa caccgccaag gccaaagcgt
      301 tcacgattga gccgagccgc cgaagatcgg cattctcccc gagatccccg atcaagcgcg
      361 cgagcagccc cgcgtgcagc acgacgaggg gcgcataaag agccggactg aacggcactc
      421 gaatgcccac gacggccggg agaatgatcg gagcgtgtgc gaacaccatg gacagggcat
      481 aacccacgaa cacggcgtgc aagactgcat cgtagagcac cccgaactgc ggcagtccga
      541 cgatcatcag cagaactcca gcgacgcaaa gccagagtga gcccgacatg atccccactg
      601 ctgagaagcg cgggagcccc gcggcccgca cggtgcggcg cgcgatgtcg aaccgcagct
      661 gccacgcccc aatcaaagcc aagggagggc ccagcatgcg agtcgcgttc ggaattccaa
      721 gcgcgcttgc gcacgccagg agcgtgctca gcagagccaa cgaaagcagc gcttgcgagg
      781 cccaacttgg cgcccttgcg agccgggaca actccagacg ttccgccgaa atcgtagcca
      841 cgaagaacac gagccaagcg ggtgcggcgg cggcaatcgg cttgccgcat gcccacgcca
      901 gattgcccgc cccgagcagc aacgtcccga gcaacatgag ccacgtgaac cccgccgact
      961 gtcgccgcac gatcgcgcca ttcacgagga ccagcagcac cgccgcgagc accgcaagcc
     1021 aggctgattc aggtctccct agcaacaagc ccatcgccga tgccgccgca accgccggcg
     1081 ctaacagcgc gatgggcctg cccagcgcca ccgcgcgttc gagcgcaatc acggtgccga
     1141 atacaccaac cacgaataag gggccatgcg cgagtgcata cttgggaccc cagccgaagc
     1201 tgattcccaa ccggcccagt ccggccaaaa ccgcggtcag cgcggcgccc gccaccacca
     1261 cgagcaacgc gcgccgtagc gctaaccggc ccaaccgagc aactccttcg ccgccaagga
     1321 cattcgatcc gggctccaaa gcggctccca cacgagctcc acctgaaccg attccaaacc
     1381 tcgaattgcc aacagctgct gttcggcttc cacgcgcacg tgctcaccca aggggcaggc
     1441 cgggctcgtc atggtgagct ttaccgtggc gtggccctgc gcatcgacat ccgcctcgta
     1501 caccaagcca agatcgacga tattgatgcc gagctctgga tcgagcaccg cacgaagtgc
     1561 cgtcatcagc tgcgttcgaa gcgacgactg gtcggtcatg attcggtgac gagtgttgcg
     1621 atgggacggc tctcgagcac gtctcacgac ctccgcccat gactcccgtc aggcgtcccc
     1681 gcggcagaag ctcgacggat cagcatcgac tcgcccgaag cggattgagc cgaagagccg
     1741 catcgccagc cgaagagact gtgcttacct cgagagacac atcgtcactc gattggccgc
     1801 gcatcccccg gaggcggcgt gcgtggcttg gtttacgcga gcgcggagat gtcggtcaga
     1861 cgccgatccg ataggatatt gtcgacgatc ttgatcacct gtcccaccac cttggcgcgc
     1921 gtagcctcgt cgagtgaatc acccggcacc ccggtgctcg gcgccagctc cggagcgcgg
     1981 agcgatccaa cggattgacg ccctcgagta cccgacgctg aaagcgagtg acgcgctgga
     2041 gcgcggagcg ctccaaccga ttgacacagc tgctcctgaa tcagcaggca ctcgctggcc
     2101 atgaccgtgt cggcctcggt ctcgtgcgat gcgcactgct cgaacatagc ccaaacacgc
     2161 gacgagattt cgagaatggc gctacgaagc tcgagctgcc caatgggacc tgccacggag
     2221 taggcgtgct gcagggcggt caaaacttgc tcgatccgaa catcctgagg aatgattgat
     2281 tgcatgatgc gacgaatgac gcgcagaaaa ctaccgatca tcgtagaccg gcaaaaccga
     2341 acttcaagct tcatttttgc ctcacgatgc ctcgattcgc tccatgcccc acgcatcgtc
     2401 gtcaacacac gggagtgata ttcaatcgtg tcgctctgcc ccgatcatcg aacaatgact
     2461 ctcgatcacg tgaataccga atgtgcaccc aagcagggag gaagcgcacc caggcggcga
     2521 tccccgcagg cgcgacgcgc cgctcactcg agtgccgcgc gtcgtgcgaa gcacaagagt
     2581 tcgtcctcgg cgtcggcgca ttgaggctca ccgtcacaaa cgtcctccca agccacggca
     2641 tcgccggcct ggcaggtgaa tgcgtcaggc atcggaccgc aatacttctc gtcttcaccg
     2701 cccgggcact ccggaacccc atgtgcgaac cctacccgaa atgggttacc tcgccaacag
     2761 tcgagtgaag ctcgaccaga actccagatt cactatcagc gttgatccgt gtgaccgctt
     2821 tgccggcttt cgtccaatgg ggatgtttgg gccgcaaggg aacgggcgcg ccgtcagtcg
     2881 cggtgctcgg gagcgcaggc catgccgccc aaccattcga cgaactaccg cgctggccct
     2941 cgcaaggacc cgtcggcttc gaacggaggc gcgaaccagg ttgaacgccc actgcggggg
     3001 ctcgtcattg cgggcgccgg cgtggtttcg agcgtgcagg ggggagcaat tggctgccct
     3061 cgagcgcacc gcgcgtctgg cgccgcgcgg gggacgcaac gaggcgagtt tcgcgcccgt
     3121 aatatcgcgg cgcgcgacaa gggcagagct gtcactcaat ccgcagctgg ctcgttcacg
     3181 gtgcactctc cctcgccgcg gaagtgaccc accgtccgca ccagtgtccg catgaaggct
     3241 ccgtaagtgg cggcgcccaa acccgaaggg tcgtagaggc gaacgtcgat gatttgggca
     3301 tcgagctcgg ccagagtgag ctcgccgtcc cgattcgcgt cgctgctcgc cagcgcctcg
     3361 aaacgcaacg aagttgcgat cgcgggatcc ggtgaagcct gcagtcggtc atagaacagg
     3421 tgatcgccgt gggtcgtgag ttgcacttcg acactcgagt ggctgcgcac gagtacgccg
     3481 ttctgctctc tgccgccttg tgacgagtgg cagtctagat agagcgtgtg cgacggaaag
     3541 ccccaggaga agtgcatcgt tctctggtcc ttcgaggcgg tgccggccac cagaagactg
     3601 aagccatgtt cgcgcatctc tgcggcgtcg gcctcgctcg cgctggagtc cagcacggtg
     3661 ctggccgata caggcgcgat ctcgtaactc acgcggtccc acgcttgcgc cgatacttcg
     3721 gggaagtcga tgatgggctt gaccccgggt tggtggttat cgaacagctt ggacccagcc
     3781 atcacggccg ctcgcttgcc caccccatcc gccacggtga tgttctggaa attcaccagg
     3841 aacttttcat aacgtaccgt ccaaccatcg atgaagccgt cttctccgct tggggcggcg
     3901 gggatctgct gctcgatgaa gtcttccccc caggtagtga acgccaagct tccggagcct
     3961 tcggggccgc cggggtcgaa gctcgagcaa gcggcgctac agctcgcgcc caacgcaagc
     4021 atgagaccgc ggcgccacca agttccacca tcgccaatcg agcttcgcat gatcagtccg
     4081 ccgcgaaaga gaattcgtaa gccgagaagg aggcgagccc ctgcgagaag gcgatgcgag
     4141 gctgcgagtc atcgggaaac ggctcgagct tcgtcggact gccgggctca atctcggaga
     4201 aatcaacgag ctcgaaccag actcgagggt cgacgcgcag ggtgatgaca ccggagcctc
     4261 ggacttcggc ggggacgaag acgcacccgg tcacgactcc ttgagccgcc gtatgttcga
     4321 tatccgcgag cttggcgagg gctcggaagt ggcgttctgt ttcagccggt ttacgcgcgg
     4381 taccgatcgc cagcgctacg gcctctccga gctgcgacgc aaacggccct tctcccgcca
     4441 tgaagctgaa actggcggag cgatagtttc cggtaactcc ctcgccctcg gcgagccagg
     4501 tctcgccggc ccacaaatcc gtgctgctgg ggatcaaagc ctggccgagg gcggtaccag
     4561 aggcgtagtg tccggggtga gcccgcgcga cgcgcacgct cagcagctcc gtaatcgacc
     4621 gccgcgcaag ccgcgctcca aatccgggtg tggaccgcgc cagcgggggt gcgccgtcga
     4681 agtagtaaaa cggcccatga ccgatcaggg cggagtcgag ctgaacttgc caaccccgca
     4741 aggtggtgaa tggcagtttg gcttccggcc gaagctcgac gcgcgtgcgc aacgagaccc
     4801 gcgctccgcc cgtggtgcct tcgttgcacg cgggtagacc cacgccgaag caccaagcag
     4861 tgaccaacgc caagctggcc catgtcgcgc gtcgccgcgt cagcattact cgtccgttcg
     4921 tcataccgaa agtccaaacg tgaaaaggaa agatcgcggc ggccccaacg tgacgtgccg
     4981 cacgggcacc aacgacgcgg cgcctccgaa ccgggaggca tatacgaact caccatcata
     5041 gaaggtgcga tcgaataggt tgaagacctc gagccccaac tcgaacgtcc gccacttgaa
     5101 ttcggcgctg gcgtccacca gaaacacgtc gtgtccgaac tcagcgtagg gcaaaggtcg
     5161 tctgcccacg taggaaaagg cagaaccgaa atgaccgctc agcggcgccc ccaaccagct
     5221 gccgagttgg ggcgtgaatg cccagtccga gcgcaccacc acccctggcg catagggtac
     5281 gagctcaccg gcgtgatatc tgcctccgct cttctcggcc gcttcaccgg agaacacggc
     5341 gtgtgcgtag gtgaagctca gttgcgacaa gaaccagctc gcggggcgcg cggcaagctg
     5401 ggccgcgatg cccgtgcggg tcgtgccagg cacccgctcg ttgcgggcgg tggcgtggtc
     5461 gaagaccaga tcgtcgctga gtgcggtgcg gaagccggcg attgaggcct ccaaccgcct
     5521 ctcatcacga aagcgtagac cgagctcata ggaaaccacc cgcgtgaagg gagtgcgctc
     5581 cccgtcttct aggctacgcg cctgcggaga acgaaagccc tcgccgtagc tcaacagtgc
     5641 atgcaacccg ggccgcagcg ccacatccaa ggtcccgcgc ttcgagagct gcgcgccgaa
     5701 c
//
LOCUS       DASTKU010000976         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_636831, whole genome shotgun sequence.
ACCESSION   DASTKU010000976 DASTKU010000000
VERSION     DASTKU010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636831"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VFQ61_34655"
     CDS             complement(<1..406)
                     /locus_tag="VFQ61_34655"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959702.1"
                     /translation="MNDLSFRTPLWTWYLFAAIVFASLVLDLWVHRGGRALSRKQAIA
                     WSVGWISVALLFGGWLGVQFGAGAATDFLTAYAIEKSLSVDNLFLFIVIFATLKIPPA
                     EQHRVLFWGILSAFVTRAVFVALGTAVLAAWHP"
     gene            437..2683
                     /gene="ptsP"
                     /locus_tag="VFQ61_34660"
     CDS             437..2683
                     /gene="ptsP"
                     /locus_tag="VFQ61_34660"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET9959703.1"
                     /translation="MLYSSNNQTEALIRGMMSFGICKLTIFEELVRLAHREVPPSELL
                     ASAANAIAAEVLADCCRIYLARDLSKLVLRVSVGSTCEAGEALEMLALGAVSHARLAG
                     IDSPESSSLAVPLMSRARCLGAIVVARGEDAGSFTELELGTLSAVASQMVALVEGAQL
                     IDVIDGTAGPRQAEPDSHERTPGELVLVGTAASPGIAIGTAAFRHIFPQQFGAHAPRA
                     SGDRAEPDRLRDALQKTQNDLLRIQAAAAGELGEEQALIFGAHLLILHDSMLLSSIDR
                     GLKLGLSAAESVDKAFSRIVKRLNNVSDPYIQERIEDVEDLRSRVLGHLLEEDVPASL
                     RAQIVVSPRTSPSVIMELKAQGALGVASELGGTTSHGVLLARALGVPAVTGVDGLLRM
                     LASGDALIVDGEAGRVILRPTRETILEYRERESAALQKRAEFSKYRTQAPQTADHVQF
                     ELRANVALGVELEVARENGASGVGLYRTEFAFIAREGLPSRDEQVRIYAKAYQAFPEG
                     PISFRILDLAGDKLVPGSGARAAQGAFHGYRSIRVLFDYPHILRTQAQAFSLAAGNRP
                     LRILIPMVSSLEEAVRIKQLVHSALSELPQSSTRPPPIFGAMIEVPAAVEIVADLARE
                     VEFFSIGTNDLVQYALVIDREDSRMSSPRDVFQPAVLRMIRRVVKAAQRAGKEVSVCG
                     EAAARPEFAIALLALDVRTLSVSPRAIPELKQKLANVTLQPLISDVEQLLESTCATVI
                     EQTLRRML"
     gene            complement(3055..3660)
                     /locus_tag="VFQ61_34665"
     CDS             complement(3055..3660)
                     /locus_tag="VFQ61_34665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959704.1"
                     /translation="MFTKLRVTASLLSGVVFLGSVSATAFAQGAGENVATPPPAVSGS
                     EAMPADPAAVAPAPTSIGSSVSTEPVIDTATTRKTFPNRPLLVTSFVLLGGTYAASAI
                     VGKLSDRDADKKLYYPVVGPWLDLNHRGCDVEACSSNTRDRVLLAADGVLQGIGAFGI
                     LLSMVVPEKTTRNWYLIGNNSFTLAPQLGTGRSGLVAVGSF"
     gene            3946..4290
                     /locus_tag="VFQ61_34670"
     CDS             3946..4290
                     /locus_tag="VFQ61_34670"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HET9959705.1"
                     /translation="MHKLVSISMLLMLGCASHPPPSDHLASAIAATRGAQEAGAPRVP
                     KAALQLKLAEEQIAEARKMMSNGDNERADYMTLRAYNDAELALALTREAAANKRAEQA
                     STVAASADAAQP"
     gene            4340..>5199
                     /locus_tag="VFQ61_34675"
     CDS             4340..>5199
                     /locus_tag="VFQ61_34675"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9959706.1"
                     /translation="MFTAIKYSAIPLLVTLSACSASTPSSLVDARAAYQRASNGPAAR
                     LSPAQLHVAETSLAIAEQTYEDEGDSANARDRAYVAERKAQLAETQARIASADLRFAA
                     FTRETARAKERNQAATRRDLDDTKAQLAGEKTLVASQSAELNAEKNRRFAAEQSAASA
                     LAALANVAPVTQESRGTVITLSGAVIFASGRAELLPGARNKLDQVATALAQAGQSGKI
                     VVEGHTDSRGSASMNEELSQRRAAAVRDYLISKGVSADRMEAQGMGPSRPIADNDTAE
                     GRANNRRVEIV"
BASE COUNT         1074 a         1611 c         1598 g          916 t
ORIGIN      
        1 tcgggtgcca cgccgccagc actgcggtgc cgagtgcgac gaacacagct cgcgtcacga
       61 aggcgctaag aatgccccag aagagcacgc gatgttgctc tgcgggtggg atcttcaagg
      121 tggcgaagat cactatgaat aggaacaggt tgtcgacact caagctcttc tcgatggcgt
      181 aagcggtcag gaagtccgtc gctgcgccag ccccgaattg cacgccaagc cacccgccga
      241 acagaagggc cacactgatc cagccaacgc tccacgcgat cgcctgcttg cgcgacaacg
      301 cgcggccgcc ccggtgcacc cacagatcca gcaccaaact cgcgaacaca atcgcggcga
      361 acagatacca agtccacaga ggagtccgaa aggacaaatc attcatagtg caccatgcaa
      421 tgcagggggg ttctccgtgc tgtattcaag caataaccag accgaggcgc tgattcgtgg
      481 catgatgagc ttcgggattt gcaagttgac catcttcgaa gaactcgttc gcctcgccca
      541 ccgggaagtc cctccgagtg agctgctcgc ttcggccgcc aacgccatcg ccgcggaggt
      601 gctcgcggac tgttgccgca tctacttggc gcgcgacctg agcaagctcg tgttgcgtgt
      661 cagcgtcggg agcacgtgcg aagcgggtga ggctctggag atgctggcgc tcggagcggt
      721 gagccacgcg cggctggccg ggatcgactc gccggagagc tcctcactcg cggtgccgct
      781 gatgtctcga gcacggtgct tgggcgcgat cgtagtcgcg cgcggcgagg acgccggctc
      841 gttcaccgag ctggagctag gcacactttc cgcagtagct tctcagatgg tggccttggt
      901 ggagggcgcg cagctgatcg acgtgatcga cggcaccgct ggcccgcgcc aggcggagcc
      961 agactcgcac gaaagaacgc ccggggaact agtgctcgtg ggcacggcgg cgtcacccgg
     1021 aatcgcgatt ggcaccgccg cgtttcggca catctttccc caacaattcg gggctcacgc
     1081 gccgcgtgca agcggcgacc gcgcggagcc tgaccgcctg cgagatgcgc tgcagaagac
     1141 ccagaatgat ttgcttcgta ttcaggccgc cgcggccggt gagctcggcg aagagcaggc
     1201 tttgatcttc ggcgctcacc tcctgatcct gcacgactcg atgctgctct cgagcatcga
     1261 ccgcggcttg aagctcgggc tcagcgcggc ggagtcggtc gacaaggcct tcagcaggat
     1321 cgtgaagcga ctgaacaacg tctccgatcc ctacatccaa gaacgcatcg aagatgttga
     1381 ggatctccga agccgagtgt tggggcatct gctcgaagaa gacgtcccgg cctcacttcg
     1441 agcacagatc gtagtgagcc cgcgcacttc gccatcggtg atcatggagc tgaaagcgca
     1501 gggcgcgctc ggtgtagcga gcgagctcgg gggtacgaca tcgcacgggg tgctgctcgc
     1561 gcgagcgctc ggcgttccgg cggtcaccgg cgtcgatggg ctgctgcgta tgctggccag
     1621 cggagacgcg ctgatcgtcg acggggaggc gggtcgagtg atcctgcgcc cgacccgcga
     1681 gacgattctg gagtatcgag agcgcgaatc ggcggcgctg cagaagcgcg ctgagttctc
     1741 gaagtatcga acccaggccc cgcagacggc tgaccacgtg cagttcgaac ttcgcgccaa
     1801 cgtggccctg ggtgtcgagc tggaggtcgc ccgtgaaaac ggggcttcgg gcgtgggcct
     1861 gtatcgcacg gagttcgcat tcatagcgcg agaaggccta ccttcccgcg atgagcaagt
     1921 gcgtatctac gccaaggcgt accaggcgtt tcccgaaggg ccgatctcgt ttcgtattct
     1981 cgatttggcg ggcgacaaac tggtgccggg cagcggcgcc cgggccgcac aaggcgcctt
     2041 tcacggctat cgatccattc gcgtgttgtt cgactacccg cacatcttgc gcacgcaagc
     2101 acaggcgttc tccctcgccg ccggaaaccg gccgctgcgc atcctgattc cgatggttag
     2161 ctcgctcgag gaggcggtgc gcatcaagca gctcgtgcac tcggccctga gtgagcttcc
     2221 ccagagttcg acgcgcccac cgccaatctt cggcgccatg atcgaggtgc ccgccgcggt
     2281 cgagatcgtc gcggacttgg ctcgtgaggt cgagttcttc tcgatcggaa cgaatgatct
     2341 ggttcaatac gcgctggtga tcgaccgcga ggactcgcga atgtcttcac cgcgtgacgt
     2401 gtttcagccg gcggtgctgc gtatgattcg ccgcgtggtc aaagcggctc aacgagcggg
     2461 caaggaggtt tcagtctgtg gtgaagccgc cgcacgcccc gagttcgcga ttgccttgtt
     2521 ggcgctcgac gtgcgcacac tcagcgtttc gccccgcgcc atcccggaac tcaagcagaa
     2581 gctcgcgaac gtcacgctgc agcctctcat ttcggacgta gagcagctac tcgaaagcac
     2641 ctgtgcaacg gtgatcgagc agacgctgcg tcgaatgtta tgacccggcg cggcgggata
     2701 ccgtgcgcaa cgctaaaccg catcaccgtc agcgagcctc tttccgcgcg gatcgaaagt
     2761 ggcctcggga gctcgatgct gaacacgcag cccttgccgg ggacatccat cacgtggagt
     2821 tccccggagt tcgccgctcg gcgtgagaag tgagcatctc acccagctgg cccaagaacg
     2881 cccaaatgcc gtgaccgagc gttccggact cccaatgact cggagcacgc tcggttaggc
     2941 gcatttttgc gcgctcgggt atccaaacac gagttataga acatagatac cgatatccgc
     3001 tcaggcggtc acgagtccag aatcacccgg cgtgacgtta cttttagttt cgattcagaa
     3061 actgcccacc gcaacgagcc cgctccggcc ggttcccaac tggggtgcca aggtaaagct
     3121 attattccca atcagatacc aattccgagt ggttttctcg ggcaccacca tgctgaggag
     3181 gatgccaaac gctccaatac cttggagtac accgtctgcg gccagcaata cgcggtctcg
     3241 agtattggat gagcaggctt ccacgtcaca gccgcgatga ttcaagtcca accacggtcc
     3301 gaccacggga taatagagct ttttatcggc gtcgcggtcg gacaacttac cgacgatcgc
     3361 cgaagcggca taggtgccac cgagcaggac gaagctggta actagcaacg ggcggttggg
     3421 gaacgtcttg cgggtggtgg cggtatcgat cacgggttcc gtagagacgc tactaccgat
     3481 actggtcggg gcgggggcga ccgctgccgg atcggcgggc atggcttcgc taccggacac
     3541 ggcggggggc ggagttgcca cgttctcacc tgcgccttgc gcaaacgcgg tcgcgctgac
     3601 actaccgagg aaaacaacgc ctgacagcag cgaggcagtg actcttaatt tggtaaacat
     3661 cgggagactc cttgcaggga aatgacaccc gaatacacgc aagaagcgcg cccacccccg
     3721 gccagccgat gatccgcaca ccgaacatgc aactcaggcg ccttctgtaa tctcgccatc
     3781 tagttcggcg catcaaactc gaatctgaca tcgagtcaaa atcacatcgc tgccctgttc
     3841 acacatattc gccaaacaga actgaccgag accagcctaa ttgtgctttg aaccacaagc
     3901 cctcaatgtg gcacgaaaca agcaatgaat cccaccgagg aagcaatgca caaattagtc
     3961 tcaatttcca tgttgctgat gcttggctgc gccagccatc cacctccgtc cgatcatctg
     4021 gctagcgcca tcgccgcgac gcgcggcgcc caagaagcgg gtgcaccacg cgttccgaag
     4081 gcggcgctgc agttgaaatt ggccgaagaa cagatcgcgg aggcccgcaa gatgatgtcg
     4141 aatggggaca acgagcgggc tgactacatg actcttcgtg cttacaacga tgctgagcta
     4201 gcgctcgcgt taacgcgcga agccgccgcc aacaagcgtg ccgaacaagc ttctacggtg
     4261 gcggcttcgg ccgacgccgc tcagccctaa tcggcactga gtctcgcata tcgctggtca
     4321 catatccaag gttactccaa tgtttacagc aattaaatat tcggcaattc cgctactggt
     4381 cacgctctcg gcctgctcgg cctccactcc atccagcttg gtagacgcac gtgccgcgta
     4441 ccaacgcgca tccaatgggc ccgcagcgcg cttgagccct gctcagctac acgtcgcgga
     4501 gacatcactc gccattgctg aacagaccta tgaggacgag ggggatagcg ccaatgcacg
     4561 agaccgtgcg tacgtcgcgg agcgtaaggc ccagctcgct gagacccagg cgaggatcgc
     4621 ctcggcagac ctgcgtttcg cggcatttac ccgggagacc gcgcgcgcga aggaacgcaa
     4681 tcaagccgca acccgacgcg acctagatga caccaaggcc caactcgcgg gggagaagac
     4741 gttggtcgcc agtcagtctg ctgagttgaa tgcggagaag aaccgtcgct tcgccgcgga
     4801 gcaaagcgcg gccagcgcgc tcgcggcgtt ggcgaacgta gcgcccgtga cgcaagaatc
     4861 gagaggaacc gtgattacgt tgtcgggtgc cgtgatcttc gcgagcggcc gcgcagaact
     4921 gttacccgga gcgcgcaaca agttggacca agtcgcgacc gccctcgcgc aagctgggca
     4981 atcgggcaaa atcgtagtcg agggacacac cgattcacgc ggatcggcga gcatgaatga
     5041 agagttatct cagcgccgcg ccgctgcggt gcgcgattac ctaatttcga aaggcgtctc
     5101 tgctgaccgg atggaagcgc agggcatggg accaagccgc cccatagcgg acaatgacac
     5161 ggcggagggc cgtgcgaaca atcgtcgcgt agaaatcgt
//
LOCUS       DASTKU010000977         3321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1533054, whole genome shotgun sequence.
ACCESSION   DASTKU010000977 DASTKU010000000
VERSION     DASTKU010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533054"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFQ61_34680"
     CDS             complement(<1..199)
                     /locus_tag="VFQ61_34680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959707.1"
                     /translation="MLRFGADLETTRVDRTPLCYVARCPVEDQEAGARPSGSARSAAV
                     SDERFQTIHPTAGGGGSFGAAR"
     gene            588..1172
                     /locus_tag="VFQ61_34685"
     CDS             588..1172
                     /locus_tag="VFQ61_34685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959708.1"
                     /translation="MRTQLGSADWSGPTSMAYNLSTLKLYIVQSGRLHRIDDENTGAY
                     SVLGAAVWGDTSAMTSIGSSLYIVSAGNLYKVNPTNGSRTILGSVGAWLGTTSMSAQR
                     WGETKGWLYIVQNDRIHRVDPDNGAWTVIGNAEWGGPTSATCYAPGSTDGASSLFVFQ
                     NDRLQKVFRDGSYEAVGGAVWTGPIVSHSISCNL"
     gene            complement(1243..1497)
                     /locus_tag="VFQ61_34690"
     CDS             complement(1243..1497)
                     /locus_tag="VFQ61_34690"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HET9959709.1"
                     /translation="MREIGRFARSSGTSPRTHYLVGDSFSVADISLYAYVHVAHEAGF
                     DLDEFPNLSMWLRRISREPGVLPMHVNPEATEDRARGPRD"
     gene            2259..2612
                     /locus_tag="VFQ61_34695"
     CDS             2259..2612
                     /locus_tag="VFQ61_34695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959710.1"
                     /translation="MRTGIKRLVGLVVGASTFVAGTALAAGAAWSNDNFISDIEVSAG
                     VTYLKFGTAPLNNPCTTANPSENVQVLLSGTADSVRSMASVATAAFLASKPLRIYYDG
                     NCTGKYAKITGVRVF"
     gene            2775..>3321
                     /locus_tag="VFQ61_34700"
     CDS             2775..>3321
                     /locus_tag="VFQ61_34700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959711.1"
                     /translation="MKSDFTRRNLAGAATRVLLLASGISAAACAMVDDPTSEPGVTAE
                     DASRKDQNLAFAEGTRLWPDSVPVCFENSPCRRRCSQNAEVECVSNSDCGTGNVCESV
                     KRCVNNNTCTRDADCAVPAEMYEDAKSLITAALDETWAANSSLRFDYKGECPLNEDPS
                     WMKVTLWDEPSGGFCSLGAGAD"
BASE COUNT          672 a          942 c         1055 g          652 t
ORIGIN      
        1 gccgcgcagc tccgaacgag ccgccaccac ccgcagttgg gtgaatcgtc tggaaccgct
       61 catccgagac cgccgctgat cgagcggagc cgctgggccg cgcaccagct tcctgatctt
      121 cgaccggaca acgcgccacg taacatagcg gtgttcggtc gacccgggtg gtctctaaat
      181 cagccccaaa gcgcagcatt ctcgccttcc gatgctggta tcaaagctgc acagcggatg
      241 ctcgcacgca gtccaattgg gggcaggcgt gcggcagcta gatccataac gaagggttca
      301 caatgacgac gattcaacga cctctcctgg gccgcttgcg cagctgcgct ctcgccgcgc
      361 tcgtattcgc agcaactctc tccggggcgc cccgtgcaca cgcaagtatt ggaacggtcc
      421 tttacgcagt ccagggggat acactctatg acataggatt tcacggggta aacctcgcca
      481 tccccgtgtc gaatgggtgg tccggtgcca catcccttag gtcgggatca actggccttc
      541 tcgtcgttca gaacgggagc ctctggagag taaacacgaa taccggtgtg cgcacacaac
      601 ttggatctgc cgactggagc ggaccgacct cgatggccta caacttgagc actctcaagc
      661 tgtacatcgt tcaaagcggc cgtttacacc gcatcgatga cgaaaacacc ggagcgtatt
      721 cggtgctcgg tgctgcggtg tggggcgaca cgtcggccat gacgagcatt ggaagttccc
      781 tttacatcgt gagcgccgga aacctatata aggtcaatcc caccaatggc tcacgcacaa
      841 ttctgggcag cgtgggggcg tggctcggta cgacgagcat gtccgcgcaa cgttggggcg
      901 agacaaaagg ctggctgtac attgtccaga acgaccggat ccatcgagtt gacccggata
      961 acggcgcatg gacggtgatc gggaatgctg aatggggcgg cccgaccagt gctacttgtt
     1021 acgcgccggg ttctacggat ggtgccagca gcttgttcgt ttttcaaaac gaccgcctgc
     1081 aaaaagtctt tcgcgacggc agctacgagg cagtcggagg cgcagtgtgg actgggccga
     1141 tagtgtctca cagcatatca tgtaacttgt aattcacaac gtgcgagcat gcggagcatg
     1201 gctgtactgc tgggttgcgc tgagtatgtt tgctctgcgc aatcagtccc gtggtcctcg
     1261 agccctgtct tcggtggcct ccggattcac atgcatcggc agcacgcccg gttctcgaga
     1321 gatgcggcgc agccacatcg agagattggg gaactcgtcc aagtcgaagc cggcttcgtg
     1381 ggcgacgtgg acgtaggcgt aaagtgagat gtcggcgacg gagaagctgt cgccgacgag
     1441 atagtgcgtg cgcggtgagg tgccgctcga gcgtgcaaag cgcccgatct ccctcaccgc
     1501 gccacacttc gaagaactcg ggcgagtcgc gactagatcg ggaaactccc ggcgaaagcc
     1561 gctctcgaga ccacaccgcc tttgaggcga cagctgtcgc cctcagagtc gatgagctca
     1621 gctcgtctga gtcgagcgat aggggggcac gcgggcggga aagcttcgta cgctcgggag
     1681 ggctcgatcg tgctgcagag aatcgagccc tatcgtgata gcgacgagtg acgccagaga
     1741 gcaatagatc tgcgtcgcgg tcctcatagg agagtcaacc acttttagac gcgcagcggt
     1801 gcgcggccgc tatggcgctc cggccgcgac gaaggcgtag cttccaagcg agcgggatga
     1861 atctggggag ccgagactcg agtgcaattg cgtgcctgaa cgcgagtcgt ccagggaagc
     1921 ggaggtgcgg gacccagtcg tggagtgagt cggcagcgat cgctcgggga cgcattcaag
     1981 ctgcggtttc gaccttttgg cgagtggcgc gcgggagagc ctggatgggc tcacggcttt
     2041 cgattgtttg cgctcccagg ggccgtcgcc gggtcacgat gcagagttgc gctcgtgaac
     2101 gcactcgacc ggacaggtct caccggaggc gagaagcgtt cggtcaccca aaaatactgc
     2161 gtaaacgccg caaattgcgg gctcttaggt tggtaccccg tttgcagtct cggtcaggcg
     2221 tcgcgcgaaa cagcgcgctt gcacaacggg agtttcaaat gagaaccgga attaagcgtc
     2281 tagttggatt ggttgttggt gcctctacgt tcgtagccgg aacggcgctg gctgctggcg
     2341 cggcgtggag taacgacaac ttcatttcgg atatcgaggt cagcgccggc gtcacttacc
     2401 tgaagttcgg tactgcgccc ctcaacaacc cttgcactac ggcgaacccc tcggaaaatg
     2461 tgcaggtgct actatcgggc accgcggatt cggtgcgcag catggcgagc gttgcgaccg
     2521 cagctttcct ggcgagcaag ccgctccgaa tttactatga cggcaactgt accggaaaat
     2581 acgccaagat cacgggcgtg cgcgtgttct agaccctcgg gtgagcgcgg tagcaggcct
     2641 ggggtgagcg ttcagccctc cgggccgctt gcgcctcgag ttcatcccaa cggaggccgc
     2701 gtcttttgcg cgaactacca gttctgggat ggaccccaga actgtccggt agttttcttc
     2761 aaaggagcat ggatatgaaa agcgatttta cccgacgcaa cctcgctggt gcggcaacac
     2821 gcgtgttgtt gctggcttcc gggattagcg cggctgcctg tgcgatggtg gatgatccga
     2881 cgtccgagcc aggggtgacg gccgaagatg cgagccgcaa agaccagaac ttggcctttg
     2941 ctgaaggcac ccggttatgg ccggattcag tcccggtttg cttcgagaat agcccctgcc
     3001 gtaggcgctg ctcgcagaat gccgaagtcg aatgcgtcag taattcggac tgcggtactg
     3061 gcaacgtttg tgagtccgtc aagagatgcg tgaacaacaa tacatgcacg cgcgacgcgg
     3121 attgcgcggt gccagctgaa atgtacgagg atgcaaagtc tttaattaca gcggctctag
     3181 acgagacgtg ggccgccaac agcagcctgc gctttgacta caagggtgag tgtcccctaa
     3241 acgaggaccc ttcgtggatg aaggtgactc tctgggatga gccctctggg ggcttctgct
     3301 ccctgggcgc tggcgcagac t
//
LOCUS       DASTKU010000978        13892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_860912, whole genome shotgun sequence.
ACCESSION   DASTKU010000978 DASTKU010000000
VERSION     DASTKU010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13892
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860912"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4636
                     /locus_tag="VFQ61_34705"
     CDS             <1..4636
                     /locus_tag="VFQ61_34705"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9959712.1"
                     /translation="APLELGRLLTGRHLVVVGGTGFLGKVWLSYLLHHFPEVGQIHLV
                     VRAKGDMSAQQRFEHEIAPSAVFDPVRQTHGANYEAWLASKVVPVFGDVVKPFCGLDV
                     GLREELRGKIDAVVNVSGVVDFDPPLDEALEVNAFGVQNLVALARDLGSVPLLHTSTC
                     YVAGNRTGVVEEKHPYEHPFPRASELERSHWEPDREISECLDVIEQARHRGNDAFRQS
                     RFLDEAKKNLRDRSEPARGKVLENEVQKVKRKFVEDRLAEMGMERANFWGFPNTYTYT
                     KAIGEQIAASSGLRFTIVRPAVVESTLQYPFPGWNEGINTSAPLIFILRQGGLQIPGG
                     NNYLDVIPCDMVASGLMLALGELLDGTEKPVYHLGSSDSNPCTMRRFFELTGLYKRKH
                     YQRTGKGGPLQSFLQSHFEGSMLSKEQFESIGPKKISDGARSLSKLLKSVAQGPAAPL
                     LKPAAKALNNFAEQQRRVGRVLEVFVPFTAEFHYIFRADNVRGAYARLTPADRARVLW
                     NPEQIDWRQWFMEVHVPGLEKWVFPLIEKKLDRPLKPVQRHESLPELLDEMADRFGLA
                     IALQRTESEGRSHLSFVEWRKRSRAVTARLRAMGVKPQDRVLLGGQNHPAWPIALFGI
                     AGAGAVAVPLDPNIDLTAFSNISSASRAGWALLDATLCKRVGPAALEGLQVMDLQAAA
                     DAGEEADFELVGSDEPCVLIYTSGTTGHPKGVQLSHENLTSLLAMLAPLFPLGKGDRI
                     LSVLPLHHTFELTCGLLLPLSRGARVVYLDELNAERLTQTLSEARITGLVGVPALWEM
                     LERRILSKVAERGPIAQWWFDVAAELNRSLGKTAGLDLGKVLFGPVHQALGGNLRFLV
                     SGGAALPAKTHRVFAGLGLHLAEGYGLTEASPVLAVSEARPGASPGHVGKPIPGVEIR
                     IAEPNAQGIGEIQARGKNVMLGYSDDPEATSKVLTPDGWLRTGDLGKVDRRGRLSIMG
                     RQKEVIVASNGENVYPDDVEARLAGIPHVAELAVLGIADGRGGERVACVVVPEFEGGT
                     RIERHARARGALDSAIAKLPAAMRPAHITFLDNKLPRTASRKVKRDELRRNIERVLAA
                     STPPPASSRPEKDPAALLVRSAIATIGRRDPAQIAAHQTLRGDLGFDSLMLLELLVAL
                     EAQAGRSVEAERLNDCHSVGEVEALLREVRRTSELRSPTTQIEKSEKPIEVPEPVREG
                     VKTWMGRVQHGFYESVLDTKVTGRAFLPHNRNVIVAANHASHLDMGLVKYALGSYGKK
                     LVSLAAQDYFFEGPTFRRFYFENFTNLAPMSRTGSLRQALRQAGDLIERGETVLIFPE
                     GTRSTDGSVHEFKPAVAYLAMQHGVDVLPVYLGGTHAALPKGASLIRRREVEARIGPP
                     IPAAELKRLTQGLGAAEAARAGARIIERAVRALSRGEVLDVQKLEPTDVAPSQKPIEP
                     SLEHVFRELHSRFKPGSVEQPVSFYFALGDSERWSVRITENSCEVKAGKVEIPADCVL
                     KTSKDMFTRIVRERYTPSPAEFMAGAIKSNNIALLFTFQKAFQLQASDAE"
     gene            4681..5748
                     /locus_tag="VFQ61_34710"
     CDS             4681..5748
                     /locus_tag="VFQ61_34710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9959713.1"
                     /translation="MHSSSTHSSTQNGGSQNGYNGVSTKPHRYWVAGATGFLGTNLVR
                     VLRGAGQHVVAVSRSGGEVDGIAVESVDVLDADAVCRSAQGMDGAFLVTGKVARDRDA
                     AEELHRLHVIGTRNALEGLRRAGVRRVVVASTSGTLAVGTDPERVYDETAEAPIEHIA
                     TWPYYRTKLYAEREALAFNDPPKFEVVIVNPSLLLGPGDVRESSTGDVRRFLSRSVPA
                     IPPGGVAFVDVRDAAEGMWLAFQKGRAGERYLLNAKNLTLRAFFERLGRISGVKVPPL
                     VMPRQRAISLGLGRLYLEAVKAIGGEPPIDPVSLEMSQYYWYCDSTKAERELGFSARD
                     PGETLRETVQDLIDRRVVFIS"
     gene            6046..6927
                     /gene="htpX"
                     /locus_tag="VFQ61_34715"
     CDS             6046..6927
                     /gene="htpX"
                     /locus_tag="VFQ61_34715"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF003965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HET9959714.1"
                     /translation="MFKRITLFVVTNLAVVLLLSIVGQILLQTGIVSPKLAGAYGPLL
                     VMAAVMGFGGSLISLLISKWMAKWSTGAQVIEAPRNSSEAWLLETVRRQAERAGIGMP
                     EVAVYDSPEPNAFATGPSKNNALVAVSTGLLQRMDRDEVEAVLGHEVSHVANGDMVTL
                     TLIQGVVNTFVIFLSRIIGNLVDSALRRGDDERGGGGIGYFATVMISQLVLGLFASMI
                     VAWFSRYREFRADAGGAQLAGRQKMINALAALKGESDSDLPKAMAAFGIRGGVLSLFS
                     SHPPIEQRIQRLRESGV"
     gene            complement(7047..8321)
                     /locus_tag="VFQ61_34720"
     CDS             complement(7047..8321)
                     /locus_tag="VFQ61_34720"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET9959715.1"
                     /translation="MLRPQMAEESSFARLMESASAQATGRATRRLRAGDVVEVTVIQI
                     SHDSVFVDVGLPSDGRIARAELQNDDGSVRVRVGDRLRATVLDPRPDAPQLAVSLGHG
                     SLDTSSLELAKMSGAPVEGEVSKVNKGGLEVQLGTFRAFCPASQVELNHVADLNVYVG
                     QKLEFKVIEVRDNGRSIVVSRRALLEDRRREAQLSAKERLAPGSEVEGTIASVNKHGA
                     VVDLDGVEGFIHLSELAGHRVQRAEDVVRIGERVRLRVLSVDDSPKGLKVRLSLKALA
                     APEPGKTAAPPQEQVLVGTVTRLLQHGILVETAQGEGLLPLRELGLAPGADHRRAYPP
                     GKQLSVVVVSRAGGKLTFSATQVARVEERQNYREFSNQGASGGGASLGSLGDLFKGKL
                     PAVAPPKPEPAKAEPRAAKSFTDIALKKKPRA"
     gene            8591..9508
                     /gene="pqqB"
                     /locus_tag="VFQ61_34725"
     CDS             8591..9508
                     /gene="pqqB"
                     /locus_tag="VFQ61_34725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HET9959716.1"
                     /translation="MLVRVLGSAAGGGFPQWNCGCSNCVGVRQGTLRATARTQESVAV
                     SSDGDAWFILNVSPEIRQQIEAFPGLHPRALRQTPIAGLVLTNGDLDHCLGVLSLRES
                     QRLVAYATDRVRRGFVDGNVFYRTLQRFEGQFSWQILELAQPQPLRDAEGHASGLSLT
                     ALPLPGKPPLHALSSERHPEDNVALRIRDDRTGRVLAYLPGVMGPSALVDEALRGADA
                     VFFDGTFWTSDELVQLGIGTRRAEDMAHWPLSGESGSLEYLKRFPDARRVLIHINNTN
                     PVLREDSRERAELDAAGVQLAYDGMEFSL"
     gene            9505..10272
                     /gene="pqqC"
                     /locus_tag="VFQ61_34730"
     CDS             9505..10272
                     /gene="pqqC"
                     /locus_tag="VFQ61_34730"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HET9959717.1"
                     /translation="MTQASGFFSTPPRPPFRAPSRESDLPLPREQFIEWLRTEGKNRY
                     HDAHPVHVAMHRGKLTRTQLQQWVLNRYYYQTRIPIKDALILSKSEDAEFRRLWIHRI
                     HDHDGERAGEGGLALWLRLADGVGLDTELVASTREVLPGVRFACDAYVTLVREGSLLE
                     AVASSLTEFFAPDLMTRRIQSWQEHYPWVKQDTLDYFRSRVTRARRDSEEAIEYVVAR
                     ATSYSLQCACVAALVKKTEILWHLVDCVAAAYGPIEP"
     gene            10282..10611
                     /gene="pqqD"
                     /locus_tag="VFQ61_34735"
     CDS             10282..10611
                     /gene="pqqD"
                     /locus_tag="VFQ61_34735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HET9959718.1"
                     /translation="MIPRAAHPRLASKAKLRHDRRSGQDVLLYPEKGLVLNATGSAIL
                     KLCTGEHSFDQICEQLAQQFQSASKPELEAHVTSFLETLAHRGLIEGIADAPIDSKPA
                     PPTERQP"
     gene            10640..11770
                     /gene="pqqE"
                     /locus_tag="VFQ61_34740"
     CDS             10640..11770
                     /gene="pqqE"
                     /locus_tag="VFQ61_34740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HET9959719.1"
                     /translation="MAESSNPAGASNLATDISDPRAYTLIAELTYRCPLRCPYCSNPV
                     DFRERKDELSTADWLRVLREAEELGVLQVNFSGGEPLLRDDLEQFIAEAKRLDLYQNL
                     ITSGLPLTRERLQGLVAAGLNGLQLSIQDSDRAGSEHIAGCKSYDHKLQVAAWVKELG
                     LPLTVNCVLHRENLRHVSEIVALAERLGADRLELANAQYLGFALQNREALLPSRELLA
                     EARAIAAREKQRLQGKMELLFVLPDYYSDRPKSCMNGWGRRYLLVTPDGLALPCHLAH
                     TLPGLEFENVRERSLEELWNSSGFRAYRGQDWMQEPCRSCDRRHTDFGGCRCQAFYLT
                     GDATATDPVCSLSPEHGRILQARERSETESGLVPLRYRGAIR"
     gene            complement(11767..12879)
                     /locus_tag="VFQ61_34745"
     CDS             complement(11767..12879)
                     /locus_tag="VFQ61_34745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HET9959720.1"
                     /translation="MKTRAAVAYEAGKPLVVEEVELEGPKPNEVLVEMKATGVCHTDE
                     FTRSGADPEGIFPVIFGHEGAGVVVEVGSQVSSLKPGDHVIPLYTPECRACKSCLSRK
                     TNLCTAIRATQGKGLMPDGTSRFSRGKTKIHHYMGCSTFANHSVLPEIALAKVREDAP
                     FDKICYIGCGVTTGIGAVIYTARVEPGANVVVFGLGGIGLNVVQGARMVGANRIIGVD
                     INPEREALARQFGMTDFVNPKTLGKDLVPHLIELTDGGADYSFECVGNTDLMRQALEC
                     CHRGWGVSVIIGVAGAGKEISTRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMDGK
                     IEIDRLITHKLPLDRINEAFDLMHSGASIRTVIEYS"
BASE COUNT         2469 a         4408 c         4453 g         2562 t
ORIGIN      
        1 cgcgccgctc gaactcgggc gtttgctcac cgggcggcac ctggtcgtgg tgggtgggac
       61 cggctttctc ggtaaggttt ggctctctta cctgctgcat cacttccccg aggttgggca
      121 aatccacctg gtcgtacgcg ccaagggcga catgtcggcg cagcagcgtt tcgagcacga
      181 gatcgctccg agcgccgtat tcgacccggt tcggcagacg cacggggcaa attacgaggc
      241 gtggctggcg agtaaggtcg tgccggtgtt cggcgacgtg gtgaagccgt tctgcggctt
      301 ggacgtcggg ctgcgcgagg agctgcgcgg caagatcgac gcggtcgtga acgtctcggg
      361 cgtcgtcgat ttcgatccgc cgctggacga ggcgctcgag gtcaacgcct tcggcgtgca
      421 gaacctggtc gcgttggcgc gtgatctcgg tagcgttccg ctgctccaca ccagcacttg
      481 ttacgtcgcg ggcaaccgca ccggcgtggt cgaagagaag cacccgtacg agcacccgtt
      541 cccgcgtgcg tcggagctcg aacgctcgca ctgggagccc gatcgcgaga tctcggagtg
      601 cctcgacgtg atcgagcaag ctcgtcatcg cggcaacgac gcctttcgcc agagccgctt
      661 cttggatgaa gccaagaaga acttgcgcga tcgcagcgag ccggcgcgcg gcaaggtgct
      721 cgagaacgag gtccagaagg tcaaacgcaa gttcgtcgag gaccgcttgg ccgagatggg
      781 catggagcgc gccaacttct ggggctttcc gaacacgtac acgtatacga aggctatcgg
      841 cgagcagatc gcggccagct ccggcttgcg cttcacgatc gtgcggcccg cggtggtcga
      901 gtcgacgctc cagtatccct tcccaggctg gaacgagggt atcaacacca gcgcgccgct
      961 gatcttcatt ttgcgccagg gcgggctgca aatccccggc ggcaataact acctcgacgt
     1021 cattccgtgc gacatggttg cgagcggctt gatgctcgcg ctgggggagc tgctcgacgg
     1081 caccgagaag cccgtctatc acctcgggtc gagcgactcg aacccgtgca cgatgcggcg
     1141 cttcttcgag ctcaccggct tgtacaagcg caagcactat cagcgcacgg gcaagggagg
     1201 gccgctgcag tccttcctgc agtcgcactt cgaaggctcg atgctctcca aagagcagtt
     1261 cgagagcatt ggtccgaaga agatctccga cggcgcacgt tcgctctcga agcttctgaa
     1321 gtcggtcgct caagggcccg cggctcctct cctcaagccg gcggccaagg cgctgaacaa
     1381 cttcgccgag cagcaacgcc gagtggggcg cgtgttggaa gttttcgtgc cgttcacggc
     1441 cgaattccac tacatctttc gagccgacaa cgtgcgcggc gcatacgcgc ggctcactcc
     1501 ggcggaccgt gcgcgcgtgc tctggaatcc cgaacagatc gactggcggc agtggttcat
     1561 ggaagtccac gtgccggggc tcgagaagtg ggtgttcccg ctgatcgaga agaagctgga
     1621 tcgaccgctc aagcccgtgc agcgtcacga gagcctgccg gagctactcg acgaaatggc
     1681 cgatcgcttc ggtttggcca tcgctctgca gcgcaccgag tccgaaggcc gcagtcatct
     1741 gagcttcgtc gagtggcgga agcgctcgcg cgcggtgacc gcgcgcttgc gtgccatggg
     1801 tgtgaagcca caggatcgcg tgctgctcgg cgggcaaaac catccggcgt ggccgattgc
     1861 gctgttcggg atcgccgggg cgggcgcggt cgcggtgccg ctcgatccga acatcgatct
     1921 gaccgcgttc tccaacatca gcagcgcgag ccgtgcgggc tgggcgctgc tcgacgccac
     1981 gctctgcaag cgggtagggc ccgccgcgct cgaaggcctg caggtgatgg atctgcaagc
     2041 agccgcagat gcaggtgaag aagccgattt cgagctcgtc ggtagtgatg agccctgcgt
     2101 gctcatctac acgagcggca ccaccgggca ccccaagggc gtgcagctct cgcacgaaaa
     2161 cctgacctcg cttttggcca tgctcgcgcc gctcttcccg ctcggtaagg gcgaccgcat
     2221 tctgagcgtg ctgccgctgc atcacacgtt cgagctgacg tgtggtttgt tgctgccgct
     2281 ctcgcgcgga gcgcgcgtgg tttacctcga cgagctgaac gccgagcgcc tcacgcagac
     2341 gctgtccgag gcgcgcatca cgggtttggt gggcgtgccc gcgctctggg agatgctcga
     2401 acgccgtatt ttgtcgaagg tggccgagcg gggcccgatc gcacaatggt ggttcgacgt
     2461 ggccgccgag ctcaatcgat cgttgggtaa gaccgccggg ttggacttgg gtaaggtgct
     2521 gttcggtccc gtgcaccaag cgctcggggg caacctccgt ttcctggtca gcggcggcgc
     2581 ggccctaccc gccaaaacgc atcgcgtgtt tgctgggctg ggtttgcacc ttgcggaagg
     2641 ctacgggctc acggaggcct ctcctgttct ggccgtttca gaggcacgac cgggtgcctc
     2701 tcccggacac gtcggcaagc cgatccctgg ggtcgagatt cgaatcgcgg agcccaacgc
     2761 gcagggcatc ggcgagatcc aagctcgcgg caagaacgtg atgctcggtt actctgacga
     2821 ccccgaggcc acctccaagg tgctcacgcc ggacgggtgg ttgcgcacgg gcgatctcgg
     2881 caaggtcgat cgccgcggtc gcctctcgat catgggccgc cagaaagagg tcatcgtggc
     2941 ctccaacggc gaaaacgtat accccgacga cgtcgaggcg cggctggctg gcattccgca
     3001 cgtcgccgag ctcgcggtgc tcggcatcgc cgatggccgt ggtggagagc gggtcgcgtg
     3061 cgtggtggtg ccggagttcg aaggcggtac ccgcatcgag cgccacgctc gagcgcgcgg
     3121 cgccctcgac tccgccattg ccaagttgcc ggcggccatg cgccccgccc acatcacttt
     3181 cctggacaac aagctgccgc gcaccgcgag ccgcaaggtg aaacgcgacg agctgcgtcg
     3241 caatatcgag cgggtgctgg cggcgagcac gcctccaccg gcatcgagtc gacccgaaaa
     3301 ggatcccgcg gcgttgttgg ttcgctccgc gatcgcgacc atcgggcggc gtgatccggc
     3361 gcagatcgcc gcgcatcaga cgctgcgtgg ggacctcggc ttcgactctt tgatgttgct
     3421 cgagctgctc gtcgcgctcg aggctcaggc cgggcgcagc gtggaggcag agcggctgaa
     3481 tgactgccac agcgtcggag aggtggaagc gctcctgcgg gaagtgcgcc ggacttcgga
     3541 gctacgctcg ccgacgacac aaatcgagaa gagcgagaag cccatcgagg ttcccgagcc
     3601 cgttcgcgaa ggcgtaaaaa cttggatggg gcgcgttcaa cacggcttct acgagagcgt
     3661 gctggacacg aaggttacgg gtcgcgcctt cttgccgcac aaccgcaacg tgatcgtggc
     3721 cgcaaaccac gcgagccatc tcgacatggg gctcgtgaag tatgcactcg gcagctacgg
     3781 caagaagctc gtctcgttgg ccgcgcagga ctacttcttc gaaggaccga cgtttcgccg
     3841 cttctacttc gagaacttca cgaacttggc gcccatgtcg cgcaccggct ctttgcgaca
     3901 agccttgcgt caagccggcg acttgatcga acgcggggag acggtgctca tcttcccgga
     3961 aggcacccgc agcacggacg gctcggtgca cgagttcaag cccgccgtcg cctatctggc
     4021 gatgcaacac ggcgtcgacg tgttgcccgt ttacctgggc ggcacccacg cggcgttacc
     4081 caagggcgct tcattgattc ggcgacgtga ggtcgaggca cggatcggcc cgccgatccc
     4141 tgcggcggag ctcaagcgct tgacccaagg tctcggggcc gccgaggccg cgcgcgccgg
     4201 cgcgcgcatc atcgagcgtg ccgtgcgagc cttgagccgc ggtgaggtgc tcgatgtgca
     4261 aaagctcgag cccaccgacg tagcgccatc gcagaaaccc atcgagccct ctctcgagca
     4321 cgtgttccgc gagctgcaca gtcggttcaa accgggtagc gtcgagcagc ccgtcagctt
     4381 ctacttcgct ctcggcgaca gcgaacgttg gtcggtgcgc atcacggaga actcctgcga
     4441 ggtcaaggcg ggcaaggtcg agatccccgc tgattgtgtg ctcaagactt cgaaggacat
     4501 gttcacgcgc atcgtgcgcg agcgctacac gccgtcgcca gcggagttca tggccggcgc
     4561 catcaagtcc aacaacattg cccttctctt cacgttccaa aaagccttcc agcttcaggc
     4621 ctcggacgcc gaatagcgca acccgcatcg tccccgcacc gagcacgatc gagcagcagc
     4681 atgcacagca gcagtacgca cagcagcacg cagaatggtg gcagccaaaa cggctacaac
     4741 ggcgtgtcga ccaaaccgca ccgttattgg gtggcggggg cgacggggtt cttgggtacg
     4801 aatttggtgc gcgttctacg aggcgccggt cagcacgtgg tggcggtctc gcgctcgggc
     4861 ggcgaagtcg acggcattgc ggtcgagtcc gtagacgtgc tcgacgcgga cgcagtgtgc
     4921 cgcagcgctc aaggtatgga cggcgcgttc ctggtcaccg gcaaagtggc gcgcgatcgc
     4981 gacgcggcgg aagagctgca ccgcttgcac gtgattggca cgcgcaacgc gctggagggt
     5041 ttacgtcgag ccggcgtgcg gcgcgtggta gtcgcgagca ccagcggcac ccttgccgtc
     5101 ggtaccgatc ccgagcgcgt atacgacgag acggccgagg ctcccatcga gcacatcgcg
     5161 acttggccct actaccgcac caagctttac gccgaacgcg aggcgctcgc gttcaatgat
     5221 ccgccgaagt tcgaggtcgt gatcgtcaat ccgagccttc tgctcgggcc cggggatgta
     5281 cgggagtctt cgaccgggga cgtgcggcgc ttcctttcgc ggtctgtccc agccattccg
     5341 ccgggtgggg tggcgttcgt agacgtacgg gacgcggccg agggcatgtg gctcgcgttt
     5401 caaaaagggc gcgcgggtga gcgctacctg ctcaacgcca agaacctcac gctgcgggct
     5461 ttcttcgagc gcttgggtcg catcagcggg gtcaaagtgc cgccgctcgt gatgccccgt
     5521 cagcgcgcga tctccctcgg cttggggcgc ctgtacctcg aggcggtcaa agccatcggc
     5581 ggtgagccgc ccatcgatcc ggtgtcgctc gagatgagcc agtactactg gtactgcgat
     5641 tcgaccaagg cggagcggga gctcggcttc agcgcacggg atccgggtga gactctgcgc
     5701 gaaaccgttc aggacctcat cgaccgtcgc gtggttttca tcagctgagg ccgcgctggg
     5761 ccgaaagcac ggtttgaaca acggctcgcg cggggcacgt cgcgggcgca taggagagtt
     5821 gcggccgatc gactccgaga gcatggcgtg cccggcacgc tagcccctcg cgcggcgtaa
     5881 caatcgggcc accgagttat tccctcgtgg ctttcgagcc cgggggccgt ggacagcgcg
     5941 gggggcggtg cttataagaa aacttcagcg gcgccccctc cgggagacgc tgcgagtccg
     6001 gtctaatcgc cccacctggt gtgggattcc ggcggaggag ttccggtgtt caagcgcatt
     6061 accctgttcg tcgtcaccaa tctcgcggtc gtcctgctgc tcagcatcgt cggacagatc
     6121 ttgctgcaga cgggcatcgt gagtccgaag cttgccggcg cctacggccc cctgctcgtg
     6181 atggcagccg tgatggggtt cggcggttca ctcatttcgc tcctgatttc caagtggatg
     6241 gcgaagtggt ccacgggggc ccaagtcatc gaggcgccgc gaaactcgag tgaggcgtgg
     6301 ctgctcgaga cggtgcggcg ccaagcggaa cgcgcgggta tcggtatgcc cgaggtggcg
     6361 gtctatgact cacccgagcc caacgccttt gccacggggc ccagcaagaa caacgcactg
     6421 gtagccgtga gcactgggct tctgcagcgg atggaccggg acgaggtcga ggcggtgcta
     6481 ggtcacgagg tgtcccacgt cgccaacggc gacatggtga ccctgacctt gattcaaggc
     6541 gtggtgaaca ccttcgtcat cttcttgtcg cgcatcatcg gtaacctcgt cgatagcgcg
     6601 ctccgcagag gagacgatga gcgcgggggt ggcggcatcg gctacttcgc gacggtgatg
     6661 atctcgcagc tcgtgctcgg cttgtttgcg tcgatgatcg tggcttggtt cagccgttat
     6721 cgcgagttcc gggcggacgc tggcggcgcc cagctcgccg ggcgtcagaa gatgatcaac
     6781 gcgctcgcgg ccctgaaggg cgagtcggat tccgatctac cgaaggcgat ggctgcattc
     6841 ggaattcggg gcggcgtact cagcctgttc agctcgcatc ctcccatcga gcagcgcatc
     6901 cagcgtctgc gcgagagcgg agtctgagct cgctcggtcg agcgtaaaac gaccccgcac
     6961 aaaactagag cgccggactc ttcgtgagcc cggcgctctt ttacttgata cgttcgcgac
     7021 gcggaggcct ggtcgaagct cttggctcag gcgcgaggct tcttcttcag tgcgatatcc
     7081 gtgaagctct tggccgcgcg cggctcggcc ttcgcgggtt ccggcttagg cggggctacg
     7141 gcgggcaact tccccttgaa tagatcgccc aagctgccga gcgaggcgcc gcctccgctg
     7201 gcgccttgat tcgagaactc gcggtagttc tggcgttcct cgacgcgcgc gacctgcgtg
     7261 gcgctgaagg tgagcttgcc gcccgcgcgg ctcaccacca cgaccgagag ctgcttaccc
     7321 ggcgggtagg cgcgccggtg atcggcgccc ggagcgaggc cgagctcgcg cagcggcaac
     7381 agcccttcac cctgcgccgt ctccaccaag atgccgtgtt gcagcaagcg cgtgaccgta
     7441 cccaccaaaa cttgttcttg cggcggagcc gcggtcttgc cgggctccgg ggcggcgagg
     7501 gctttcagcg aaagccgcac tttcaggccc ttcggtgaat cgtcgacgct caacacgcgc
     7561 agccgcaccc gctcgccgat gcgcaccacg tcttccgcgc gctgtacgcg gtgccccgcc
     7621 aactcggaga gatggatgaa gccctcgacg ccgtcgagat cgacgaccgc gccgtgcttg
     7681 ttgacgctcg cgatcgtgcc ttcgacctct gagcccggcg cgagccgttc cttcgcgctg
     7741 agctgcgctt cgcgacggcg atcctccagc aatgcgcggc gcgacaccac gatgctgcga
     7801 ccgttgtcgc gaacctcgat gaccttgaac tcgagcttct gcccgacata cacgttcaga
     7861 tcggcgacgt ggttcaactc cacctgggag gccgggcaaa aggcgcgaaa cgtgccgagt
     7921 tgaacttcga ggccaccctt gttgaccttg ctgacctcac cctcgacggg tgcgccgctc
     7981 atcttggcga gctcgagcga gctggtgtcg agtgacccgt ggccgagtga aaccgcgagc
     8041 tggggcgcgt cggggcgcgg atcgaggacg gtcgcgcgca ggcggtcgcc cacgcgtacc
     8101 cgcacgctgc catcgtcgtt ctgtagttct gcgcgcgcga ttcgaccatc gctcggcagc
     8161 ccgacgtcca cgaacacgct gtcgtggcta atttgaatca cagtcacttc caccacgtca
     8221 cccgctcgca agcggcgtgt cgcgcgcccg gtggcttggg cggaggcaga ctccatcaat
     8281 cgcgcaaagc tcgactcttc ggccatttgg gggcgcagca tagaggagaa tcgcccgcct
     8341 tcaacccgcc tctgaattac ccgggataag taacggagtg agcgcccggg agggtgtacg
     8401 tctggaaatc cgggctccag gttgactgag caccgtcagc gtggaatggt cagcgacgtc
     8461 acaaagaagg agtcgattca tggtttggga gatgccgact ttcgttgaaa tcgagatgaa
     8521 cgcagaaatt ggctcctatc aggaagatta cgacggaatc tgaaccgaag cggtagctcg
     8581 ggtaacggcc ttgctcgttc gagtcctcgg ctccgcggct ggtggtgggt ttccgcaatg
     8641 gaattgcgga tgttctaact gcgtcggggt tcgccagggc acccttcgcg ccaccgctcg
     8701 cactcaagag agcgtcgctg tcagcagtga tggcgacgct tggttcatcc tgaacgtctc
     8761 gccggaaata cggcaacaaa tcgaggcgtt ccccgggtta catcctcgcg cgttgcgcca
     8821 aaccccgatc gcggggttgg ttttgaccaa cggtgacctg gaccactgcc tgggtgtttt
     8881 atcgctacgc gagtcacagc gcctcgtggc gtatgcgacg gaccgagtcc gccggggctt
     8941 cgtggatggc aacgtcttct accgcacgct gcagcgcttc gaaggacagt tctcgtggca
     9001 aatcctggag ctcgcgcagc cgcagccgct acgggacgcg gagggccacg cttcggggct
     9061 gagcctgacg gcgctcccgc tgccagggaa gccgccgttg cacgcgctct cgagtgagcg
     9121 ccatccggag gacaacgtgg ctttgcgcat tcgggacgat cgaaccgggc gcgtgctcgc
     9181 gtacttgccg ggagtcatgg gcccctctgc gctcgtggac gaagctttgc gtggcgcgga
     9241 cgccgttttc ttcgacggca ccttctggac cagcgacgaa ctcgtgcagc tcggcatcgg
     9301 aacgcgcaga gccgaggata tggcgcattg gccgctctcg ggggagtccg gcagcctcga
     9361 gtacctgaag cgcttccccg acgctcgccg tgtcctgatc cacatcaaca acacgaaccc
     9421 ggtgctgcgc gaggattcgc gcgagcgcgc cgagctcgac gcggcgggag tacagctggc
     9481 ttacgacgga atggagttca gcctgtgacc caagcttctg gtttcttcag cacgccacca
     9541 cggccgccct tccgtgcgcc ctcgcgagag tccgacttgc cgctgccacg cgagcagttc
     9601 atcgagtggc tgcggaccga aggcaaaaat cgctatcacg atgcacatcc cgtgcacgtg
     9661 gcgatgcatc gcgggaagct cacgcgcacg cagctgcagc agtgggtgct caatcgttat
     9721 tactatcaga ctcgcattcc catcaaagac gcgctgattc tgtccaagtc cgaagatgcg
     9781 gagttccgta ggttgtggat tcaccgcatt cacgaccatg acggtgagcg cgcgggggaa
     9841 ggtggcctcg cgttgtggct gcgcctggcc gacggcgtgg ggctcgatac cgagctggtc
     9901 gcgagtacgc gagaggtgct gccgggcgtg cgctttgcgt gtgatgcgta cgtaacgctc
     9961 gtgcgtgaag gatcgctact cgaggcggtg gcctcctcgt tgaccgagtt tttcgcgccc
    10021 gatctgatga cccggcggat ccaatcctgg caagagcatt acccctgggt gaagcaagat
    10081 accttggatt acttccggtc tcgggtaacc cgggcgcgtc gtgactccga agaggccatc
    10141 gagtacgtcg tggctcgtgc gacgagctat tccctgcagt gcgcttgtgt cgcggctctg
    10201 gtgaagaaga cggaaatcct ctggcatcta gtggactgcg tggcggcagc ttacggccca
    10261 atcgagccct gaggcctaag catgattcct cgcgccgctc acccgcgttt ggcaagcaaa
    10321 gctaagctgc gccatgaccg ccggtcgggc caggacgtgc tcctttaccc tgagaagggc
    10381 ttggtcttga acgccacggg ctctgcgatc ctcaagctct gcacggggga gcacagcttc
    10441 gaccagatct gtgagcaact cgctcagcag ttccaatcgg cgtccaagcc cgagctcgaa
    10501 gcgcacgtca cgagtttctt ggagacactg gcccaccgcg gcttgatcga gggcatcgcg
    10561 gacgccccca tagattcgaa gccggccccg cccacggaac gccagcccta acccaataac
    10621 tccggagcag ctcagactcg tggccgaatc cagcaacccc gcaggcgcga gcaacctggc
    10681 aacggatatt agcgatcctc gcgcctatac gttgattgcg gagctaacgt atcgttgccc
    10741 cctgcgctgc ccttattgct ccaatccggt ggatttccgc gagcgcaaag acgaactctc
    10801 caccgctgat tggctgcggg tattacgtga agcggaggag ctcggcgttc tacaggtgaa
    10861 tttcagtggc ggagagccgc tgctgcgcga cgatctggag caattcatcg ccgaggccaa
    10921 gcgactcgac ctgtatcaaa acctgatcac gagcgggttg cccctgacgc gcgagcggct
    10981 gcaggggctc gtggcggcgg gcctgaatgg attgcagctg tcgatacaag actccgatcg
    11041 ggcgggctcc gagcacattg cgggctgcaa gagctatgac cataagttgc aggtcgcagc
    11101 ttgggtcaaa gagctcgggc tgcccctgac tgtcaactgc gtcttgcacc gtgagaacct
    11161 gcggcacgtg agcgagatcg tggcgctcgc ggagcggctc ggcgcggatc gactcgagct
    11221 cgcgaacgcg cagtacctcg gcttcgcgct gcaaaatcgc gaggccttgt tgccctctcg
    11281 tgagcttttg gcggaagcac gcgccatcgc agcccgcgag aagcagcggc tgcagggcaa
    11341 gatggagctc ctgttcgtgc tgcccgacta ttactcggat cgccccaagt cctgcatgaa
    11401 cggctggggc cggcgttatc tgctcgtgac ccccgatggc ttggctttac cctgccatct
    11461 ggcgcatacg ttgccgggtc tcgagttcga gaacgtccgg gagcgctcgc tcgaagagct
    11521 ctggaactcg tcgggtttcc gcgcctatcg cggacaggat tggatgcagg agccgtgtcg
    11581 ctcgtgtgac cgccgccata ccgatttcgg cggctgccgt tgtcaggcgt tctatttgac
    11641 gggggatgct actgccaccg atccggtgtg ctctctctcg cccgagcatg ggcgcatcct
    11701 tcaggcccga gaacggagcg aaactgagtc tggtttggtt ccgctccgtt atcgaggcgc
    11761 gattcgttag ctgtattcga tcacggtgcg gatcgaagca cccgagtgca tcaggtcgaa
    11821 ggcctcgttg atgcgatcga gcggcagctt gtgcgtgatc aagcgatcga tctcgatctt
    11881 gccgtccatg taccaatcca cgatcttcgg tacatcggtg cgcccgcgcg caccgccgaa
    11941 agccgttcct ttccaaactc gacccgtcac gagttggaag ggccgagtcg agatctcctt
    12001 acccgctcct gccacgccga taatcacgct cacgccccag ccccggtggc agcactcgag
    12061 cgcttgccgc atcagatccg tgttcccgac gcattcgaag ctgtagtcgg cgccaccatc
    12121 cgtcagctcg atcaggtgcg gaaccaagtc tttaccgagg gtcttcggat tgacgaagtc
    12181 cgtcatcccg aattggcgcg ccagcgcttc gcgctccgga ttgatatcca cgccgatgat
    12241 acgattggcc ccgaccatgc gcgcgccttg cacgacattc aagccgatgc ccccgagccc
    12301 gaacaccacc acatttgcgc cgggttcgac gcgtgccgtg taaatcaccg cgccaatccc
    12361 ggtggtgacg ccgcagccga tgtaacaaat cttgtcgaag ggcgcgtctt cccgcacctt
    12421 ggccagcgca atctccggca gcacgctgtg gttcgcgaac gtggagcagc ccatgtagtg
    12481 gtggatcttg gttttgccgc gcgagaagcg gctcgtgcca tctggcatca gccctttgcc
    12541 ttgggtggcc cggatcgcgg tgcagaggtt cgtcttgcgg ctcaggcacg acttacaagc
    12601 gcgacactcg ggcgtgtaga ggggaatcac gtggtcaccg ggtttcagcg agctgacttg
    12661 ggaccccacc tccaccacca cgccggcgcc ttcatgtccg aaaatcaccg ggaagatccc
    12721 ctccggatct gcaccggagc gcgtgaactc gtcggtgtgg caaacgccag tggccttcat
    12781 ttcgaccagc acctcgttcg gcttcggccc ttccagctcg acctcttcga cgaccaaagg
    12841 ctttccagct tcgtacgcga cggcagcacg agttttcatg agaccggcag cctacaacat
    12901 cgggtggccc gacgtctgct gcttcaccgc acagcatggt acgaacttag cgaaacaccc
    12961 tggaacaact cacatcagcc agagcctcgc tctcgcgcgt cggctagcga ttggcgccgg
    13021 acacgaacca cgagacacgc gatgcaagca gagagccacc agagctctgg actagactca
    13081 tcgcttccgg ccgggcccgc agagcagccg ccggtaggct ttagtggcac acacacatct
    13141 cgtgaatcga catggctaca agcccaaccg actggacatt cttcccgcac gtcgcaggtt
    13201 tgagtacaga tcggttcagg ctttgctcct agcaagcatt gattggtggc gcactgattg
    13261 gcttcaacac accgttctcc gaatcctgac agaccaggga ggccgcagta gcccggacca
    13321 cgcagcggga gtacacagcg ctgcgcgtat gcacaatccg cgtcttgcca gcattgagct
    13381 gcgcattcgt gattggttag acagacgcgg gttagaccgc attcttcgtt ctgaatgcaa
    13441 ctacagccta ctcctccggg gcagtcgtgt ggacatccgg cgggaccaca ctccgggcat
    13501 tcaggcccag gacaaagggc acttgcagca caacgacctt ctacgcagac ttcgtccgct
    13561 gagcagaatg atgcgccgca tctgctgaca cattctcctt cagcacagac agtgccaggc
    13621 gggcaggact catcctcaca acgcgggtac aagtgacata cgccagccaa atcgtcgtcg
    13681 agaacatgcg tgatgtaacc tgcatagtag ggatacatca cactttcgtt cgcttcgagt
    13741 cctgtatcgg aacagacaag agcaccggaa accccctccc gctcacaagg atgcatgagg
    13801 ccgagagcat gccccgtctc gtggatgact acgctcagaa tgctgcggtg ctccgcatcc
    13861 ggaacacggt ctaatgacca gctgaagtac tc
//
LOCUS       DASTKU010000979        20528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1980156, whole genome shotgun sequence.
ACCESSION   DASTKU010000979 DASTKU010000000
VERSION     DASTKU010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 20528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20528
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980156"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1052
                     /locus_tag="VFQ61_34750"
     CDS             255..1052
                     /locus_tag="VFQ61_34750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959721.1"
                     /translation="MQELGRSLGFSAGYRVAFAGRLIALTSVTLGVLATTSKASAQLE
                     LSARTGYGHGFGKLSNAPTGGNLRDVTSGQLPIWFDAGFRVTPSVAILGFFSYGWGFS
                     GDYADDICKSADLSGITCDTWSTDMRVGAQVHYHLAPNKKSPGPWFGLGVGYEVLRFG
                     LDLVEFRSVEQKGSGLEFFNVQFGVDIPGNGAIAGGPFLALSVGQYSSGTLSCSGRGC
                     EDTSTITLDIDKKAPHAWLFLGVRLRLQPTELRPPAPKRREYEPPNE"
     gene            complement(1084..2844)
                     /locus_tag="VFQ61_34755"
     CDS             complement(1084..2844)
                     /locus_tag="VFQ61_34755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HET9959722.1"
                     /translation="MQTSYTADPAPMVYEGRLYLYTSHDEDVTVKNFFTMNDWRLYST
                     VDMVNWTDHGSPASYKTFSWGTGDAWAPHGIARNGKFYLYVPLNNSTGSKIGVAVADS
                     PLGPFKDALGKALIATGGGNIDPTVFIDDDNQAYLYWGNPDLWYVKLNSDMISYAGSP
                     TKVNLTTAGFGTRSNSDRATAYEEGPWFYKRDALYYLVFPADGTPEKISYSTSSGPLG
                     PWSFGGDIMAKQTGNASSFTNHPGVIDYKGKSYFFYHNAALPGGGGFKRSVCVEEFTY
                     ASNGKIPTIQMSKSGPAAVATLDPFQQVEAETIAFSSGLKTETCSDTGGGLNVTSISN
                     GDYIKMKNVDFKDGVKSFEARVSSSTSNAKIELRLDSQAGALIGTCDVSGVTAWATKT
                     CAVSGASSTHDLYLGFVGGTGDLFKFNSWKFVGSTPSNGGTGGTTGTTGGSAGNSSSG
                     GSNGGVSGSTSAMGGTRAATGGETVSAGASGGASATGGSNAGESGSSSNSTAAGGGSS
                     AHSGGASSGLGGGSGGLAAGGTGLASGNPGTPRSENGCGCRMSSRSSQSSLLASAVLI
                     GLALQRRSGTRRRGSRSRSI"
     gene            3654..4880
                     /locus_tag="VFQ61_34760"
     CDS             3654..4880
                     /locus_tag="VFQ61_34760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9959723.1"
                     /translation="MIGEISIPNPKSVDATGIRVGRRPRFITLGTSLAVLAVTAAGVA
                     KALSSTTLSLGNGQAVAFGTLAALHALLGSVGLAWIERRGTRKALYLHFAVAILIAEG
                     ALALSRGVGFLLVLSLISQSVVYLSLASTVCVTVLYAVTAFVFFGVDGKWPSEAVGVA
                     SSIAFVLVFSQLLGRENRARGEVERLAAELADANQRLHAYASAVEELAATKERNRIAR
                     EIHDGIGHYLTVVHVQLEAAQATFLPAPAKAQLAISKAQELTREGLAEVRRSVGVLRG
                     AAAETAAVPSLIRAIEKLANECTRDGISAALEVSGQPRPLTEATGTTLFRAAQEALTN
                     VRRHARASHVRIELDFRDSSAVRIEVEDDGAGTANVGHGFGLMGLRERAELGGGRFTA
                     SSEPGRGFRLQLELPT"
     gene            4877..5533
                     /locus_tag="VFQ61_34765"
     CDS             4877..5533
                     /locus_tag="VFQ61_34765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9959724.1"
                     /translation="MIRVLIADDQALFREGLRTALCALGLDVVGEAGNGEEAVRLAAE
                     LEPHVVLMDLRMPVLDGVAATRRITQKAQAPRVLVLTTFDDDESVFEAIRAGALGYLL
                     KDARAAVLVDAIRLAARGEAFLQPRIATKLVGEFARITPPGKRNPASDLGLSERELSV
                     LRVLAQGASNKEIAAALGITEGTVKNHVTNILLKLEVEARTEAALKAHELGLLDPRSR
                     "
     gene            5694..6650
                     /locus_tag="VFQ61_34770"
     CDS             5694..6650
                     /locus_tag="VFQ61_34770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959725.1"
                     /translation="MVQIGRSRIGVLAMALGVACSSSETEKTDETPLEVEQFLEDFHA
                     SRYSQASARAAALDAAAQANPEHGRIAFDRALAHDWHTAEWRRDPSQDANAIQTERQG
                     LASLFQTAYEKNPDDPRVGCFLGLQLVGAGRALQQEQLMQQGFDVLKRAVDAWPEFNL
                     FCLGIAYDRLPASDPDYALALKAALDNIEVCYGAKLDRENPNVDLTPYLAQRTSKGPK
                     RACWNDPIAPHNGEGFFLWVGDLFVKQGNVAAARIAYNNVKLMPEYKGWPYRDVLEER
                     LNSDLEAKSALYWDDDRDNDPTFAGDEVNRRCVICHAATADE"
     gene            6972..8750
                     /locus_tag="VFQ61_34775"
     CDS             6972..8750
                     /locus_tag="VFQ61_34775"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9959726.1"
                     /translation="MHYPARGRVCAHLFAPVLALTVSCSGSIPDDTAETPGGPGAGGS
                     AGPPGAGALGGGGNNTVPGRGGAGSGAGVGGAAGGSLSSNPGASGVALKGTPAFHRFV
                     RLTHEQWEASVRDLLRLAAVPGLSAGFAGDPPNGSFTNNERALFVSSTLWGDYQRAAE
                     TLAAQITSDTQALSKLTGGSTDSAAFIRTFGRRTFRRPLTQAEEQRYQTLFAAGPSVV
                     GSGNAFADGVRLVLESMLQSPKFVYRSELGNDGAPLGGFEMASKLSFLIRNTTPDDTL
                     LDAAASGELDSAQGVLARATAMLETPAAKEAIGRYHAELFGLSRYGSIDKNRTKFPAY
                     SEALNVEFERADRMFFDRVFMLGRGLREILTSPVAFVSTASAPLYGVKVSGSGLVETQ
                     LGPERPGFLTRLGYLAYNANLSEPDPIHRGVDILNRLMCLDLLPPANVDIPPVPAAQP
                     GQTNRERVTAHTGDGTCGQGCHSTLINPVGFAFENFDALGQFRTTDNGKSIDTSGVVA
                     LKDGLKSFNGAPELATVLASAPSVHGCYVKHMTEFALARDTTTADRGLVDAIEGSSMN
                     EDASIKTMLLQIIAHPSFLTRTGGAQ"
     gene            8747..10198
                     /locus_tag="VFQ61_34780"
     CDS             8747..10198
                     /locus_tag="VFQ61_34780"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9959727.1"
                     /translation="MSSNLPRVNRRAFLRAAGTLGVALPFLESLPERSAFAQTAKPVF
                     GFFICTACGVAQKDGQDPETFWPTAEGALTTESMTAFAADRSTGVLADHASRLLIVRG
                     INYPYGLSGCGHAQGLAQCLTSSKPNGTNNTTTSSGPSIDTVIAKQLNPNGVEPMTLY
                     SGMKGGYINEKLSFSAAGQVRAAEGNPYNVYQRLVGLMPATGGSTGGSTGGSSTADRL
                     ALRRKTVNDLVREELNSLRNLSILSKADKDRLDQHFQAIRDVENTMMSMGLACAPDKL
                     DVQAIQAMNSGQAFKQNGKVEEVCKLQMDLVTLAFSCNLTRVATLQCGDGTDATRYVI
                     DGQTLERFHWISHRIQSDGSSGTAIAGAAAMHTKIDRLRMGTFKYMLDKWAQYTTPGG
                     PLFDSAFAMWTSHVAAGPSHSFRNVPIIIAGSAGGYLKQGQYLNAGGVSNNKLYNTLA
                     SAMGCKGENGGPLENFGPSDLQGGQIAALRAAT"
     gene            10394..12868
                     /locus_tag="VFQ61_34785"
     CDS             10394..12868
                     /locus_tag="VFQ61_34785"
                     /inference="COORDINATES: protein motif:HMM:NF028510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HET9959728.1"
                     /translation="MFKLRLRSKLIGLVIAPLLFVAALVTGLMTRQKHELAASMTEVM
                     RDNARRELTRVVQDVLVLCETAQEDSLAELEREIERLDRALDPNLALRASPSTRGAAF
                     SQLETVTNAVVLLFGRDEQGAVGLVAGYGAVRRESGASPGDSNSGSTGSAVPAEVTQA
                     AQSALESGVERTALVTLGTLPYMVRSSPVRSPAGPIIGVKIVGVPFHGAEGLKRRISE
                     IRLGKTGYVWVLGSRDNQRGHYIVSKDRKRDGESIWNAKDANGRLFIQDFITRAETAR
                     TRGGRAEPFFGSYPWQNAGETVAREKIAAVGYFESWGWVIGASTYQDETEGSIEQVRA
                     SMGSLVNRTLLLCALSALVFLAVAASLSSRMVRPLTEMASTARSLAQGHTRVRIAHQS
                     EDEIGILADAFRSLVDYLTEAARAARSISMGQLDVTIRPRSSNDALSAEMAHAVGVLN
                     ALTKNLGELIVSTRAGHFSDRADGSKYAGGYRELLLGMNGLLDAVIEPVVEVNRVLER
                     LAAKDLTVKARPGFAGDYAVMMNALNKAVENLRQSLDRVAKASVQVASSAEQIANTSQ
                     RVTAGAEAQAASLERTSSALQEFAANTQHNTDSARQAQEIVDAAKSAAMTGATEMHEM
                     IEATHGMGTAVQATVPIIRDINDIAFQTNLLALNAAVEAARAGEAGRGFAIVADEVRH
                     LALRSKQAAHKTEKLIGESVQLAERGAALSERVNETLCQVLGAVDEVSGIVAEISENS
                     AAQAESLDRTRVANREIGEATMRAAENARESSAAAAALATQSRQLAELVREFVLDDSR
                     PATQQTHATAPSGAAPILRLHRKASA"
     gene            13656..14705
                     /locus_tag="VFQ61_34790"
     CDS             13656..14705
                     /locus_tag="VFQ61_34790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959729.1"
                     /translation="MANPSPNRILFAAGMAFTAACVAFLAAVSGVPAKLRMVPWVDAI
                     ASAGRFTNWLGLLVAAIAVFASCILLGYWAHLPPRTPKSATAASGSRQFTEWVRGIVK
                     AEWLTITKWLLTIVLFGAASFAYTELKEHFQKTEEERVRRRELARSTTEAISQFRAAL
                     IEFGSDCLASLGRERLQAAAPNEVILSSECSANYARVVDGWTRSTWLLPLLIAELQAD
                     ECSRRASDETSGALRQAACKRLGGVGALGESSLEYRHLIQHYAALRHSRAAADLERLR
                     DSAEALLSTTRKVSCALLFIQSKHLPVPTCYCTNLFTSPGQELDASCKAPAAGMTADG
                     TEMDPIRLNEFERAQ"
     gene            complement(14735..14926)
                     /locus_tag="VFQ61_34795"
     CDS             complement(14735..14926)
                     /locus_tag="VFQ61_34795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959730.1"
                     /translation="MNALRGQEFRVVEFSVQATHIHLIVEAEGRGAPRIGLCTGQFQE
                     HPTLEGRRGELTDPSLVVK"
     gene            15419..16288
                     /locus_tag="VFQ61_34800"
     CDS             15419..16288
                     /locus_tag="VFQ61_34800"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9959731.1"
                     /translation="MSELGGLMDEIEQQSGYQEILDADFGRFGWFCSRRRERFQVKSP
                     PEPVLALVLAGRKRVQGLSIGPGELIIIPSGFSFEVVNVPESADVPYRSFVVHVAPQA
                     ATLLEQREVASACSDVPWSATRPRVLRPTRSTLLALLHFARTLSEPEVHLELLRHRLE
                     DVLLSILLAVNLGAESAPRSHAASDPVAAIRRLIRRDPAHSWLASQAAQQLGMSEPTL
                     RRRLAAAQTSFREILTDERMRLARSLLRDPSLSVMEVALRCGYQSPSRFAAQFARIQG
                     TLPSRFAELGHSS"
     gene            16359..17255
                     /locus_tag="VFQ61_34805"
     CDS             16359..17255
                     /locus_tag="VFQ61_34805"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET9959732.1"
                     /translation="MQKQSLTLVLLVLGCFGGFGTGCQETTAAAPLRLAGFKNPESVL
                     YDRTRDVYWVSNMGEGAPIARDGDGYISRLTPDGALDARYISSDRPDTTLNAPKGMAV
                     LGQELFVADIDCVRVFDADTGRPLREISFPDAEFLNDLALSPDGTLYVSETGMDASLQ
                     PTGKDAIYRVRNGSAPERWLTGRELGNPNGLTATVEALWVATFGTAELLQIGYDGTTL
                     ARHSAPQGGLDGIEPTADGGFLVTSWEAKAVYHVTPTGEFRIERGALESPADLGFDST
                     RERLLVPLMSLNQVLIEPIQPK"
     gene            17341..17706
                     /locus_tag="VFQ61_34810"
     CDS             17341..17706
                     /locus_tag="VFQ61_34810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959733.1"
                     /translation="MLRFIEDYAKHAALNFGQALQGIRYLLSHPDIDTRAPRGTLRHT
                     AHSVPLRLRRVANDLFFAALPPQWHHTQDELSAMRTKPLTRWFQYGYCAWRFDESGEP
                     KPNLVGVDRRWDPRCNTNP"
     gene            18203..18439
                     /locus_tag="VFQ61_34815"
     CDS             18203..18439
                     /locus_tag="VFQ61_34815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959734.1"
                     /translation="MTSTRQGKPERDRTDESLACERTKTDDELFKRSGGVEEAADAVV
                     TTAREHADEVVRRSRQEADAQLLARVPMAARGVR"
     gene            18436..19326
                     /locus_tag="VFQ61_34820"
     CDS             18436..19326
                     /locus_tag="VFQ61_34820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9959735.1"
                     /translation="MTSERKQEDSQLQEERTAADENLWSEREARRKFLAALLASEREH
                     TDEHLRSERAQADAAIGSRDNFLAMASHDLRNMLGAIAMSAASLTNIQCDEVVQQAIA
                     RDARRIQRNTARMARLVGDLVDIVSIEAGHLAVAPRRHDVVELLRETMEVFQPLAAAK
                     QIWIGTDVKRGSLLARYDHERVLQVLANLVGNALKFTAEGGRIHILVEPIDAFVQFAV
                     ADTGRGIGTELPAIFDRFWQLADKSARSGLGLGLYISKCIVEAHGGKIWAESRVGEGS
                     TFYFTLPAADAPSVTNATAK"
     gene            20024..20284
                     /locus_tag="VFQ61_34825"
     CDS             20024..20284
                     /locus_tag="VFQ61_34825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959736.1"
                     /translation="MALARVRLAHSALGVTETECGWIHLNELAQQRGVPEIQIKAELY
                     RVRKLLMRVIAEKQCGWMPIENRYRARLLRIGTSDIVVEPGD"
BASE COUNT         3802 a         6442 c         6420 g         3864 t
ORIGIN      
        1 aaggagccgc tctgcttgcg gtcgagctct gcttgcggtc gagctctgct tgcggtcgag
       61 ctctgcttgc ggtcgagctc tgcttgcggt cgagctctgc ttgcggtcga gctctgcttg
      121 cggtcgagct ctgcttgcgg tcgagtgccg gccgtcgagc cgcagggggg tgacatttat
      181 ccagccttgc tgacttagtc cgtcttcggg cgcgaaatcc aggttagatg ccagcgtcag
      241 gagcaggata gtcgatgcaa gagctcggga ggtctttggg tttcagtgca ggttatcggg
      301 tggctttcgc tggcaggttg attgccctca cgtccgtgac actcggcgtg ctcgcgacga
      361 cttcgaaggc gagcgcacag ctcgaattgt ctgcgcgcac ggggtacgga catggtttcg
      421 gaaaactgtc gaacgccccg accggtggga acctgagaga tgtaacctcg ggccaattgc
      481 cgatctggtt cgacgccggc tttcgggtaa caccaagcgt cgccatcctc ggcttctttt
      541 cttacggctg ggggttcagc ggggactatg ccgacgatat ttgcaagagc gctgacttga
      601 gcgggatcac gtgtgacact tggtccaccg acatgcgcgt cggcgctcag gttcactatc
      661 acttagcccc gaacaagaag agccccgggc cctggtttgg gttgggagtt ggctatgaag
      721 tcttgcgttt cgggttggac ttggtcgagt tccgaagtgt cgaacagaag ggttcgggcc
      781 tcgagttctt caacgtgcag tttggcgtcg acatccccgg taacggtgcc attgccggag
      841 ggccgttctt ggcgctcagc gtcggccagt attcgagtgg cacgctgagt tgttcaggcc
      901 gcggttgtga agacacgagc acgatcacgc tcgacatcga caaaaaggct ccccacgcgt
      961 ggctgtttct cggcgtgcga ttgagattgc aacctacgga gttgcggcct cccgcgccca
     1021 aacgacgaga gtacgagccg ccaaacgagt aagactcggc tgcccgctct tctcgaacag
     1081 tagtcaaatc gatctcgacc gcgagcctcg acggcgtgtg cccgagcgcc gctgaagtgc
     1141 gagcccgatc aggacggccg aagcgagcag ggaggattga ctcgagcgag agctcattcg
     1201 acagccgcag ccgttttcac tcctcggggt gccgggattg ccgcttgcca atccggtccc
     1261 ccctgcagcc aaaccgccgc ttccgccgcc cagccccgag ctagcacctc ccgaatgggc
     1321 cgagctacca ccgcccgctg cggttgagtt cgagctcgag ccggactcgc cagcattgct
     1381 tccccccgtc gccgacgcac ctccgcttgc accggcgctg acggtttcgc cgcccgtcgc
     1441 agctctggtg cctcccatgg cgctcgtgga tccagagact ccgccgttgc tacccccaga
     1501 actggagttt cctgccgagc cacccgttgt cccggtggtt ccccccgtgc ccccgttgga
     1561 cggtgtggag ccgacgaact tccacgagtt gaacttgaat agatcgccag taccacccac
     1621 gaaaccaaga tacaggtcgt gggtcgagct ggcgccgctc accgcgcaag tcttcgtagc
     1681 ccaggctgtg actcccgata catcgcaagt accgatcaga gcgccggctt ggctatccaa
     1741 gcgaagctcg atcttcgcgt tgctcgtcga ggaagaaacc cgagcttcga acgactttac
     1801 cccatctttg aagtccacgt tcttcatctt gatgtaatca ccgttgctga tggacgtgac
     1861 attcaggcca ccccccgtat cgctgcaggt ctccgtcttg aggcccgagg agaaggcgat
     1921 tgtttcggcc tccacctgtt gaaacggatc tagcgtcgcg accgccgccg ggcccgactt
     1981 cgacatctga atggtcggga ttttgccgtt agaagcatac gtaaactctt cgacacacac
     2041 cgaacgcttg aatccaccgc cgcccggcag cgcggcattg tggtaaaaga aatacgactt
     2101 gcccttgtag tcgatgacac cggggtggtt ggtgaaactc gacgcattgc cggtctgctt
     2161 cgccatgatg tcgcccccga agctccaggg cccgagtggg ccgctactcg tcgaatagct
     2221 gatcttctcg ggagttccgt cggcggggaa aaccagatag tagagtgcat cacgcttgta
     2281 gaaccacggc ccttcttcgt aggccgtcgc gcggtccgaa ttcgagcgcg tgccaaagcc
     2341 ggccgtggtc agattcacct tcgtcggact gcctgcgtac gagatcatgt ccgagttcag
     2401 cttgacgtac caaagatccg gattccccca atacagatag gcctgattgt cgtcatcgat
     2461 gaacaccgtc ggatcgatgt tcccgccgcc ggtcgcgatc agcgctttcc cgagcgcgtc
     2521 tttgaacggc cccaacgggc tatccgcgac cgccactccg atcttcgaac cggtcgagtt
     2581 gttcagcggc acatacaggt aaaacttgcc gttcctcgcg atgccgtgcg gcgcccaggc
     2641 atcccccgtc ccccaactga acgtcttgta actcgcaggt gaaccgtggt ccgtccaatt
     2701 caccatgtcg accgtggaat acaagcgcca gtcgttcatg gtgaagaagt tcttcaccgt
     2761 cacgtcttcg tcgtgcgatg tatagagata caacctacct tcgtacacca tcggcgccgg
     2821 gtccgcggta tagctcgtct gcacgattgg gttgtccgca agagcggatg gagcaaacgc
     2881 ccccaccact gccgcgagca gcgcgcctac gaaccggggg cgactgattg tgcaactgcg
     2941 ctgacggcct cggccaagtt gacgaacgtt agcgcagcgt aggcttgatg cgttcgagag
     3001 attcatagca tgttctcggc ggtgcagtcc tgctcccaca ctcatggagc gaacccgaga
     3061 ctcttcacta accgagtcga gaccacggcg ccagcgtcaa tgccatggat gactcgagcg
     3121 caaaggcatt gctcctgatg caacccggtt gtgatcgaga actgacaaag ggatgcactt
     3181 tctcaaatgg gaagtgccgt tcggcggcac tgcgcacccg cgttgcctcg cacgccatgc
     3241 ctcacacccc atgcctcatc catcgcgccg gtcgactttt gtgcttctga gctgcggcca
     3301 ctccgtacac gggcgcgccg cgcgtctcaa ggagccaaac gcgcagccgc agccgccctc
     3361 gctccctccc aacaccgcgc gggccctcga agcatcgagt cacgcgcact cgaccatgag
     3421 gcgagtcgtc gtgcacggcc caatcgcttc ctccgcccgc tcggactctg ctcctcggtg
     3481 caatgcatgc gtacgcgctg aaagcgcgca tgtatccgcg ccctgaaaca aagcaaggtc
     3541 gagccactgg cacggtgtgg acgccgttgc aaatggttcg gtgacttaga gccggcccga
     3601 ttgctccgcc gcgaccttgg cgcatactgg gagcggagcg gaaagccctg actatgatcg
     3661 gcgagatatc gattcccaat cccaagtccg tggacgcgac ggggattcgg gtggggcgca
     3721 ggccgcgttt cattacgttg gggacttcgc tcgcggtcct cgcagtgacc gcggcgggcg
     3781 tagccaaggc gctgtcttcg acgacgctga gcctcgggaa cggacaagcg gttgcctttg
     3841 ggacattggc ggcactgcac gcgctgctcg gcagcgtggg gcttgcctgg atcgagcgcc
     3901 gcggtacgcg caaagcattg tatcttcact tcgcggtggc gatcctgatt gccgaggggg
     3961 cgctcgctct gagccgcggg gttggctttt tattggtgct ttcgctgatc agccagagcg
     4021 tcgtgtactt gtcactcgcg agcacggtgt gcgtgacagt tctgtacgcg gtcaccgcct
     4081 tcgtgttctt cggggtagat ggtaaatggc cgtcggaagc ggtcggcgtc gcttcgagta
     4141 tcgcgttcgt gctggtgttc tcgcagttgc tcggtcgtga gaatcgcgcg cggggggagg
     4201 tggagaggct cgccgccgaa ctggcggacg caaaccaacg gctccatgcc tatgcgagcg
     4261 ccgtagaaga gcttgcggca accaaggagc gcaatcgcat cgcgcgggag atccacgacg
     4321 gcatagggca ctatctgacc gttgtgcacg tacagctcga ggccgcgcag gccacgttct
     4381 tgccagcgcc ggccaaagcg cagctggcca tcagcaaggc ccaggagctc acgcgggagg
     4441 ggttggccga ggtgcggcgc agcgtgggcg tgttgagggg ggccgccgcg gagaccgcgg
     4501 cagtgccgtc gctcattcgt gcgatcgaga agctcgccaa tgagtgcact cgtgatggga
     4561 tctccgccgc tctcgaggtc tccggtcagc cacggccgct gacggaagcg accggcacca
     4621 cgctctttcg cgcggcgcag gaagcgctca ccaatgtgcg ccgccacgcc cgggcgtcgc
     4681 acgtgcgcat cgagctggat tttcgagatt cgagtgcggt acggattgag gtcgaagacg
     4741 acggcgcggg aactgcaaac gtggggcatg gcttcggctt gatggggctg cgcgagcgcg
     4801 cggagctcgg cggagggcgg tttacagcct cgagcgagcc gggccgcggg tttcggctcc
     4861 aactggagct acccacgtga tccgcgtgtt gatcgccgat gaccaggccc tattccgtga
     4921 aggcctgcgg acggcgcttt gtgcgctggg cttggacgta gtcggtgagg ccgggaacgg
     4981 tgaggaggcc gtgcggctcg ctgccgagct cgagcctcac gtcgtgctca tggatttgcg
     5041 tatgcccgtg ctcgatggag tggccgcaac ccggcgcatc acccaaaaag cgcaggcccc
     5101 gcgggttctg gtgctcacca ccttcgacga cgacgagtcc gtgttcgagg ccatccgcgc
     5161 gggtgcgctc ggttacttgc tgaaggatgc tcgggcggcc gtgctcgtgg acgcgatccg
     5221 actggccgcg cggggggaag ccttcctcca gccccgcatc gcgaccaagc tggtcggtga
     5281 gttcgcgcgc atcactccac ctggtaagcg caaccccgcc agtgatctcg ggttatccga
     5341 gcgggagctc tcggtgttgc gggtgctggc gcaaggcgcg agcaacaaag agatcgcggc
     5401 cgcgctcgga atcaccgaag gcaccgtgaa gaatcacgtg accaacatac tgctgaaact
     5461 cgaggtcgaa gcccggaccg aggcggccct caaagcccac gaactcggtc tgctggaccc
     5521 gcgaagccgc tagcccagcg ccccgagggc tttgtcgcga tctcgctcgc gctgcaccaa
     5581 tgccatgtct ccaccgggtc atgtctcgcg catgaccctg agcatgactt gcggctgatt
     5641 gtggtggtgc tgccccacac ctatgtagcc aatcgcaacg taggaggatt cgaatggttc
     5701 agatcgggcg aagcaggatt ggcgtgctcg cgatggccct cggcgtggcg tgcagctcat
     5761 cggagaccga gaaaacggac gagacgccgc tcgaggtcga gcagtttttg gaagacttcc
     5821 acgcctcgcg ctacagccag gcaagtgcgc gtgcggccgc gctcgacgcc gcggcacagg
     5881 cgaacccaga acacggtcgg atcgccttcg atcgcgcgct cgctcacgac tggcacaccg
     5941 ccgaatggcg gcgagatccc agtcaagatg ccaatgccat tcagacggaa cggcaggggc
     6001 tcgccagctt gtttcagacc gcgtacgaga agaaccccga cgacccgcgc gtgggctgct
     6061 ttctggggct acagctcgtc ggcgcgggtc gcgccctgca gcaagagcaa ctcatgcagc
     6121 agggcttcga tgtcttgaag cgcgccgtgg atgcctggcc cgagttcaat ttattttgtt
     6181 tgggcattgc ctatgatcga ctaccggcga gcgaccctga ctacgcactt gccctaaaag
     6241 cggccctcga caacatcgag gtatgttacg gcgcgaagct ggatcgtgag aatccaaacg
     6301 tcgatctcac cccgtatctt gcccagcgaa cgagcaaggg tccgaagcgc gcttgctgga
     6361 acgacccaat cgctccgcac aacggtgaag gcttcttcct gtgggttggt gacctgttcg
     6421 tcaaacaggg aaacgttgcg gccgcgcgta ttgcctataa caacgtcaaa ttgatgcccg
     6481 agtacaaagg ctggccttac cgcgatgtgc tcgaggagcg cctgaattcc gatctggagg
     6541 cgaagtccgc cctgtactgg gacgatgacc gtgacaacga cccgacgttt gccggagacg
     6601 aggtcaatcg gcgctgcgtg atctgtcacg ccgccacggc agatgagtga atccgcggga
     6661 aacccgccag ttcaggggct ggcggcgttc gttggggtga cgtacgaccc tgcggagtta
     6721 gtcaagctcc atacctctag agagcccttc gatcgcgtgt gttgggtgtg ctcgaatcca
     6781 aagaaaattg taccaaaagg cttcatctgc ctcgatttag gccaattctc gtggcgtcag
     6841 atgctcgagt cgggcgtggt tcatttttgt tcgacggatc cgacaggcgt catgcactgc
     6901 atctgagaac cgctcagccg tcgcttcgct gaggcattcg ctcatcaccc acgcgagaga
     6961 gacgactact catgcactac cctgctcgag ggcgcgtgtg cgcgcattta tttgccccgg
     7021 tgctggccct cactgtcagc tgtagtggga gcattccgga cgacactgcg gagacccctg
     7081 gagggcccgg cgcaggaggg tccgccggac cgccaggggc tggtgctctc gggggtggcg
     7141 gaaacaacac cgtcccaggt cgtggaggag caggcagcgg tgcgggtgtg gggggcgccg
     7201 ccgggggctc gctttccagc aacccaggtg cgtcgggcgt tgcgctgaag gggacacccg
     7261 cctttcaccg cttcgtgagg ctcacgcacg agcagtggga agccagcgtg cgggacctac
     7321 tgcggctcgc cgcggttccc ggtctatctg ccggtttcgc gggtgatcct ccgaacggga
     7381 gcttcacgaa caatgaaaga gcgctgttcg tgagctccac gctttggggt gactatcagc
     7441 gcgccgcgga gacgcttgcc gcgcaaatca cctccgacac tcaggccctc tccaagctca
     7501 cgggcggttc aacggactcg gcggcgttca tccgcacctt tggacggcgc accttccggc
     7561 gcccgctcac tcaggctgaa gagcagaggt atcaaaccct gtttgccgcg gggcccagcg
     7621 tggtgggcag tggaaacgcc ttcgcggacg gtgtgcgcct ggtgttggag tccatgctgc
     7681 agtctccgaa gttcgtgtac cggtcggagc tcgggaacga tggcgctccg ctcggcgggt
     7741 tcgagatggc gtccaagctt tcgttcctga tccgcaacac cactcccgat gacactttgc
     7801 tcgacgccgc cgcgtcgggg gagctcgact cggcccaggg cgtgttggcg cgggcaactg
     7861 caatgctcga gactccggcg gccaaggagg cgattggccg gtaccacgcg gagctgtttg
     7921 gcctctcgcg ctacggctcg atcgacaaga atcgcacgaa gtttccagcc tactcggagg
     7981 ctctgaacgt cgagttcgag cgcgccgatc gaatgttctt cgatcgagtg ttcatgctcg
     8041 gacgcggctt gcgcgagatc ctcacgtccc cggtcgcctt cgtgagtacg gcaagcgctc
     8101 cgctgtacgg ggtgaaggtg agcggttccg ggctcgtcga gacccagctt ggccccgaac
     8161 gtccgggctt tctcactagg ctgggatacc tcgcttacaa cgcgaacttg agcgagccgg
     8221 atccgattca ccgcggtgtg gacatcctca accggctcat gtgcctagac ctcctgcctc
     8281 ccgccaacgt cgatattccg cccgtgcccg ccgcgcagcc tggccagacc aatcgcgagc
     8341 gggttacggc gcacaccggc gacggcactt gcggtcaggg ctgtcactcc acgttgatca
     8401 accccgtcgg ctttgccttc gagaacttcg atgccctcgg ccagttccgt actacggaca
     8461 acggcaaatc aatcgacact tcgggagtcg tcgccttgaa agacgggttg aagtcattca
     8521 acggagcgcc cgaactcgcc acggttttgg cttctgcgcc cagcgtgcac ggttgttacg
     8581 tgaagcacat gaccgagttc gccttggccc gtgacaccac gacggccgat cgcggtctcg
     8641 tcgacgccat cgaagggtcg agtatgaacg aagatgcctc catcaagacg atgctcctgc
     8701 agatcatcgc ccaccccagc ttcctcaccc gcacgggagg tgcgcaatga gctcgaacct
     8761 accgcgcgtc aatcggcggg ctttcctgcg ggccgcgggc acgttgggcg ttgcgctacc
     8821 gttcttggag agcttgccgg agcgctccgc gttcgcgcag accgccaagc cggtgttcgg
     8881 cttcttcatt tgcacggcct gcggcgtcgc gcagaaggac ggccaagatc ccgaaacgtt
     8941 ctggccgacg gcggaagggg cgctcacgac cgagtccatg acggcctttg ccgcggatcg
     9001 ctcgaccgga gtgctcgccg atcacgcctc gcggctcttg atcgtgcgcg gcatcaacta
     9061 cccgtacggg ctctccggct gcggtcatgc ccaagggctg gcgcagtgtc tgacctcgtc
     9121 caagccgaac ggcaccaaca atacgacgac gtcttcgggc ccctcgatcg ataccgtgat
     9181 tgccaagcaa ctcaacccga acggcgtcga accgatgacg ctctattcgg ggatgaaggg
     9241 cggctacatc aacgagaagt tgtccttcag cgctgccggt caggtgcgcg ccgcggaggg
     9301 taatccgtac aacgtttatc aacggctggt cgggctaatg ccggccaccg gcggcagtac
     9361 gggtgggagc accggtggtt cgagcacggc cgatcgcttg gcgctgcgtc ggaagacagt
     9421 caacgacctg gtgcgagagg agctcaattc actgcgcaat ctgtcgatct tgagcaaggc
     9481 cgacaaggat cgactggacc aacactttca ggccatccgc gacgtcgaga acaccatgat
     9541 gtcgatgggc ctcgcgtgtg cccccgacaa actcgatgtc caggcgattc aggccatgaa
     9601 ctcgggccag gccttcaaac agaatggcaa ggtcgaagag gtctgcaaac tgcagatgga
     9661 cctcgttacg ctggcattca gctgcaacct gacgcgggtc gccactctgc aatgtgggga
     9721 cgggacggac gcgactcgct acgtgatcga cgggcagacg ctcgagcgct ttcactggat
     9781 cagtcatcgt atccaaagcg atggctcctc cgggactgcg atcgcgggcg ccgcggcgat
     9841 gcacacgaag atcgatcgtc tccgcatggg cacattcaag tacatgctcg acaaatgggc
     9901 tcagtacacc acgcccggtg gcccgctctt cgacagcgcg ttcgcgatgt ggaccagtca
     9961 cgtggcggcg gggccgtctc atagcttccg gaacgtcccg attatcattg cgggcagcgc
    10021 cggcggctac ctcaagcagg gccaatactt gaacgcgggc ggtgtttcca acaacaagct
    10081 gtacaacacg ttggctagcg ccatggggtg taagggagaa aacggcggtc ccctcgagaa
    10141 cttcggaccg agtgatctgc aagggggtca gattgcagcg ctgcgcgcgg ccacctgagg
    10201 ttgtcggacc cgcatcgagt cttgctggga gtgcgctgcg ccgctccgga ccccccaccc
    10261 ggcggaatcc gaatctgact cgcgttcacg ggtgatgcgg gttcctgatt agacggggtg
    10321 ggtctggcgc tgcgtcttag ccacaacagt cttatcggtg atgctcgtcg ggccgtaaac
    10381 tgcgtcagcc cgagtgttca agttacgact gagatccaag ctaattggct tggtgatcgc
    10441 gccgttgctc ttcgttgcgg cgttggtcac tgggctcatg acgcgtcaaa aacacgagct
    10501 tgccgcgagt atgacagaag tcatgcgcga caacgcccgc cgtgaactta cccgggtcgt
    10561 gcaggacgtg ctcgtgttgt gcgagaccgc gcaggaggac agcctcgctg agttggagcg
    10621 cgaaatcgag aggctggacc gggccctcga tccgaacctt gctttgcgtg cgagtccctc
    10681 cacgcgcggg gccgcgttca gtcagctcga gaccgtcacc aacgcggttg ttctcctgtt
    10741 tgggagggat gagcaaggcg ccgtcgggct ggtcgccggt tatggagcgg ttcggcggga
    10801 atcaggggcg agtccgggtg actccaactc tggctcgact ggttcggctg tccccgcgga
    10861 agtgacccaa gccgcgcaga gcgccctcga gagcggcgtc gaacgcacgg ccctggtcac
    10921 gttgggtacg cttccgtaca tggtccgctc ttcacccgtt cgtagccctg ccggcccgat
    10981 catcggcgtg aagatcgtgg gcgtgccctt tcacggagcc gaaggcttga agcgccgcat
    11041 cagcgaaatc cgactcggca agaccggtta tgtgtgggtg ctcgggtcac gcgacaatca
    11101 gcgcgggcac tacatcgtct ccaaagaccg taagcgagac ggcgaatcga tttggaacgc
    11161 caaagacgcc aacgggcgcc ttttcataca ggatttcatc acacgcgccg agacggcgcg
    11221 gactagggga gggcgcgcgg agccgttctt tggctcctat ccttggcaaa atgccggaga
    11281 aactgtggct cgggagaaga tcgcggcggt aggttatttc gaatcttggg gctgggtaat
    11341 cggcgcgagc acctatcagg atgaaaccga gggttcgatt gagcaggtgc gcgcgagcat
    11401 gggctcactc gtcaatcgca ccctgctgtt gtgcgcattg agtgccttgg tcttcctcgc
    11461 ggtcgccgcc tctttgagca gtcgcatggt acgcccgctc accgagatgg cgagtacggc
    11521 ccgttcgctc gcgcagggcc acacccgagt gcgcatcgcg caccaatccg aggacgagat
    11581 aggtattctg gccgacgcct ttcgctcgtt ggtcgattac ctgaccgaag cggcacgagc
    11641 ggcgcgatcg atctcgatgg gccagctcga cgtcacgatc cggccgcggt cctccaatga
    11701 cgcgctctcc gccgagatgg cacacgccgt cggagtcctc aacgctctga ccaagaacct
    11761 gggtgagctg atcgtctcca cgcgtgccgg gcatttctcc gaccgtgccg atggttccaa
    11821 gtacgccgga ggctatcgag agctcctgct cggaatgaac ggtctgttgg acgcggtgat
    11881 cgagcctgtc gtcgaggtca atcgagttct ggagcggctc gcggcaaagg acctgaccgt
    11941 gaaagccagg cccggctttg cgggcgacta tgcggtcatg atgaatgcgc tgaacaaggc
    12001 cgtcgagaac ctgcgccaaa gcttggaccg ggtcgcgaaa gcctccgtac aggtggcgtc
    12061 ttctgcggag cagatcgcca acacgagtca gcgagttacg gccggcgccg aggcccaagc
    12121 cgcttcgcta gagcgcacct cgagtgcgtt gcaagagttc gccgccaaca ctcaacacaa
    12181 cacggatagt gcacgccaag cccaagagat cgtggacgcg gccaagtcag cggccatgac
    12241 gggggcgacc gagatgcacg agatgatcga ggcgacccac ggcatgggca cggccgtgca
    12301 ggcgacggtg cccatcattc gtgatatcaa cgacatcgcc tttcagacca atctgctggc
    12361 tctgaatgct gcggtagagg cggctcgtgc cggcgaagcg ggacgcggat tcgctatcgt
    12421 cgccgacgaa gtcagacacc tggcgctgcg aagtaagcag gcggctcaca agaccgagaa
    12481 gttgattggc gaatccgtgc agctggccga gcgtggcgcg gccttgtccg agcgggtcaa
    12541 cgagacgctc tgccaagtac tgggtgccgt cgacgaggtg agcggcatcg tggccgagat
    12601 ctcggagaac agcgcggccc aggccgagag cttggaccgc acgcgcgttg ccaaccgtga
    12661 aatcggagag gccaccatgc gtgctgcgga gaacgcccgc gagtcctcgg cggcggcggc
    12721 cgcgctcgcg acccagtccc gtcagctcgc ggagctcgtc cgagaattcg tgctcgacga
    12781 ctcgcgaccc gcgacccagc agacccacgc tacggctccc agcggcgcgg caccgatcct
    12841 gcgtcttcac aggaaagcca gcgcctaagt cgtcgcaacg gcgtagagcc gctcattcgc
    12901 cgcttgcgat ccccgccacg aaagcgtccg cgaaggggta agtcgtgcgg gcctcgtcag
    12961 cgcagatcag caggtctccg accgaaaagt gcagtgactg aaacgtcatc ggtccagatt
    13021 cagccgcgcg ttcccacgcg cgagagggca actcctgaac tgaggtattg cttccctgcg
    13081 tcacctgcca agtagagcta tgcagaacaa tccgattgct ccgcaacctc agggcaaccg
    13141 ttcccgtgca gggtgcctcc agcaccaccc gacagaccaa cgctgctccc ccaatattcc
    13201 acgccgcgcc gctcggtcca cccccctgcc actgacgcaa agactgctcg cgtttgcctt
    13261 ccaacgacga gtaacaatcg aacccagaga ctcgcgcccg ctccgcctcc cgagtgtcgg
    13321 tctctccaga gggggcgggc gaagcgaccg gcgtcctggt cgttaccgca ggtggcggcg
    13381 tcgcgctcgg ggctgctgct cgggtcgggc atcccgatcg agcgccgccc tcgtggacga
    13441 gagccggatc gcgcgaagcc gctttaggac atgcacagcc gcaggcgagc cacccgaggg
    13501 tgatgtgaac tagtgcccgc cgcaaaaaca tcgtgcccct gagtgaccga cggcgcaccg
    13561 cgcgtcaata ctcgggagag gattgcgtgg gtgtgatccc cattgcggta tcccgaccat
    13621 cccgctagcc tgtcgtccgg cagcgggtca catccatggc taatccctcg cccaatcgga
    13681 tcctctttgc ggccgggatg gcgttcacgg ctgcgtgcgt tgcgttcctg gctgcggtgt
    13741 ctggagtgcc ggcgaagcta cgtatggttc cttgggtgga tgccatagcg agcgcgggcc
    13801 ggttcaccaa ctggttgggt ctgctcgtgg ccgcgatcgc ggttttcgcg agttgcatcc
    13861 tactgggata ttgggcccac ctcccgcccc ggaccccgaa gtctgcgacc gcagcgtccg
    13921 gatctaggca atttaccgag tgggtccggg ggatcgtcaa agccgagtgg ctcacgatca
    13981 ccaaatggtt gctcacgatc gtgctcttcg gtgcggcaag cttcgcctac accgaactca
    14041 aagaacactt tcagaagact gaagaagaac gtgtgcgccg ccgagaactc gcgcgcagca
    14101 cgaccgaagc catctcgcag tttcgggcgg ctttgatcga gtttggttca gactgcctag
    14161 cttcattggg tcgggaacgc ctccaggcgg ccgctccgaa cgaggtcatt ctcagcagtg
    14221 agtgttccgc caactacgcg cgtgtggtgg acggatggac acgtagcacc tggctccttc
    14281 cgctcctgat cgccgagctg caagcagacg aatgcagccg gcgagcctcc gacgagacgt
    14341 ctggcgctct gcggcaggcg gcctgcaaaa ggctgggcgg ggtcggtgca ctaggtgaat
    14401 catctctcga atatcgccac ctcattcaac actacgcggc tctgcgccac tcacgcgccg
    14461 ctgcggattt ggaacgactc cgagattcgg cagaagcgct actcagcacg acccgcaagg
    14521 tcagctgcgc cctacttttc atccaaagta aacacctgcc tgttcccacc tgctattgca
    14581 cgaacctctt caccagcccg ggacaggagc tcgacgccag ctgcaaagcg cccgccgctg
    14641 gcatgaccgc agatgggacg gagatggatc cgattcgcct caacgaattc gagcgggctc
    14701 agtaagctca cgctgcgtcc cacaaacttg tgaatcattt gacgaccagg ctcggatccg
    14761 tgagctcccc gcgccggccc tccagggtcg gatgctcctg aaattgccca gtacataaac
    14821 caatgcgcgg cgcaccgcgc ccttctgctt ccacgatgag gtggatatga gtggcttgaa
    14881 cggagaactc tactacccgg aattcctgcc cgcgtagggc gttcaccctg ccgatcaccc
    14941 gccgaaccac ggcgcacaca acctgccgcc tcaaacatcg aacgcccacc cggaccgtga
    15001 cgtgcaccgg ctcgtaacgt cggtgaccgc agcgccgcgt atgcggtgtg gatttgcccc
    15061 ggcgcttgcg cccggcaccg gcgcgtctac cgccccttga ggaaacgtcg gaatcgatcg
    15121 cgcgtgcttg tctggtagca agaaccgatc actttgcggc tggtggctcc gtgacgcttg
    15181 ctcgttgcgg ttgcgcgcgt tgcggttgcg cgagccgact gaccaacacg aggggtagtg
    15241 ctggttgcga acgctccggc gcttgcacgg ccgacttctc gtcaacgcac agcaccacag
    15301 cgttcaatcc tatcgcgaat catggactcg ggacactagt ccgaggccgg caaacccacg
    15361 ccgcctgtgt caaactccgg gcgccatgcc aatgcccgtg ccgcaagtcg aaccactaat
    15421 gagtgagctc ggcggcttga tggatgagat cgagcagcag agcggttatc aggagatatt
    15481 agacgcagat ttcggtcgat ttggctggtt ctgttcgcgc cgccgcgaac gattccaagt
    15541 caaatccccc cctgagcccg tactcgccct ggttttggcc gggcgcaagc gcgtgcaggg
    15601 gctgagcatt gggcccggtg agctcatcat catcccttcg ggattcagct tcgaggtcgt
    15661 caatgtgccg gaatccgcgg atgtgcccta tcggtcattc gtggtgcacg tcgcgccgca
    15721 agccgcgacg ctgctcgagc aacgggaagt cgcttctgcc tgctcggatg tgccctggtc
    15781 cgcgacgcgg ccccgcgtct tgcggccgac gcgcagcact ctgcttgcct tgctacactt
    15841 cgctcggacg ctttccgaac ccgaggtgca cttggagctt ctgcgccacc gtctggaaga
    15901 cgtgttgctc agcatcttgc tcgccgtcaa tcttggcgcc gagagtgccc cacgctcgca
    15961 cgccgccagc gaccccgttg ccgccattcg acgcctaatc cggcgggatc cggctcattc
    16021 gtggctggcc agccaggccg cccagcaact cggcatgagc gaacccacat tgcgtagacg
    16081 ccttgccgcc gcccagacct cgtttcggga gatcttgacc gacgagcgaa tgcgactcgc
    16141 acgctccctc ctgcgagatc caagcctgag tgtcatggag gtggctttgc gctgtggata
    16201 tcagtccccg agcagattcg cagcccaatt cgcgcggatt cagggcaccc ttccgtctcg
    16261 tttcgccgaa ctcgggcact ccagttgatg agcggagcgg gcgcaaacct gaccgtttcg
    16321 ggcgcggctg cctcgagcct gtgcggcaaa tctaccgcat gcaaaaacag tcgctcacgc
    16381 tcgttcttct cgtgctgggt tgttttggcg gcttcggtac cggttgccag gagaccaccg
    16441 cggcggcgcc ccttcgcctt gccggcttca agaaccctga gtccgtactc tacgaccgca
    16501 ctcgcgacgt ctactgggtt tcgaatatgg gagaaggcgc gccgattgcg cgcgacggtg
    16561 acggatacat ctcgcgtctc actcccgacg gtgccctcga tgctcgctat atctctagcg
    16621 accgccccga taccaccctc aatgcaccca agggcatggc cgtcctcgga caagagttgt
    16681 tcgtggccga tatcgattgt gtgcgggtgt tcgacgccga cacgggtcgg cccctcagag
    16741 aaatcagctt ccccgacgcc gaattcctga atgacctggc gctctctccc gacggcaccc
    16801 tctatgtttc ggaaacgggc atggacgcga gcctacaacc caccggcaaa gacgcgatct
    16861 atcgcgtgcg gaacggcagc gctcccgaac gctggttgac cggcagagag ctcggtaacc
    16921 ccaacggcct cacggccacc gtcgaggcgc tttgggtcgc gaccttcggc accgccgagc
    16981 tcctccagat tggctatgac gggaccacgc tcgcgcggca ttccgccccc cagggcgggc
    17041 tagatggcat cgaacccacc gccgacggcg gattcctggt cacgagttgg gaggccaaag
    17101 ccgtctatca cgtgaccccc acgggggagt tccgtatcga gcgtggagcc ctcgaatccc
    17161 cagcggatct tgggttcgat agcactcgag agcgactctt ggtcccattg atgagcttga
    17221 atcaagtgct catcgagccg attcaaccca agtaaacgcg ctcgcgctgc cggacgaaat
    17281 gagtctttcg agtgcgcctc gaggttgaca cgcgaaggag tccggcctcc ttatgtggct
    17341 gtgttgcgct ttatagagga ctacgccaag cacgcggcct tgaacttcgg gcaggcactt
    17401 caagggatcc ggtacctgct atcccatcca gacatcgata ctcgggctcc gcgcggaacg
    17461 ctccgtcaca ctgcccattc cgtaccactg cggctgcgcc gagtcgcgaa tgacttgttc
    17521 ttcgcggctc tcccgccgca atggcaccac acccaagacg agctctccgc catgagaacc
    17581 aagcctctca cgcgctggtt tcaatacgga tattgcgcct ggcgcttcga cgaaagcggc
    17641 gaacccaagc caaacctcgt tggcgtggat cgcagatggg atccgcgctg caacacgaac
    17701 ccttagcgcg gccgcatcgg caagcgtctg ttcgagtggt cgatagcttc gagtcagcgc
    17761 tggggcgcca gcgcggtgcg cgctcggtgc cccgccgact gctgaaacaa atgggaatgg
    17821 cgcggtggtc atgcggcgga gccgtggacc ctgccaagcc catgaggaga accaaaccgc
    17881 ggaaacgcga gcgtagcgca gaatggcaga ctgccccgtc gcgcggtggt cattgcccac
    17941 atgacacgag cttcagcgga gagagtatgt ggtgtttgcc cgtcgttggg cgtatcggcc
    18001 gtcgccaggc agacgcccgt gaactattac gagtaaactc cgcgagccat tccaagatac
    18061 ttccgactca ccggggaccc gtttgacggc tcgagtcata aaaacgaaag taccgtacag
    18121 gaaccagaac ccaagtcgcg agctgatgcc agggccggaa tcggcattcg gatttccgcg
    18181 tgccagggtg gtagggtgag ccatgacttc caccaggcag ggaaagccgg agcgggacag
    18241 gacggacgag agccttgctt gcgaacggac caagacggac gacgagcttt tcaagcgaag
    18301 tgggggagtt gaggaagccg cagacgcggt cgtcacgacg gcacgcgagc acgccgatga
    18361 ggtggtcaga cgttcgcgac aagaagccga tgcgcagctt ttggcgaggg tgccgatggc
    18421 ggcgcgtgga gtgcgttgac gagcgaacgg aagcaggagg acagccaact tcaagaggaa
    18481 aggaccgctg cggacgagaa cctatggagc gagcgagagg ctaggcgcaa gttcctggct
    18541 gcactgctcg cctccgagcg cgaacatacg gatgagcacc tgcgctcgga gcgcgcgcag
    18601 gcggacgccg caatcggttc ccgagacaac ttcttggcga tggcgagcca cgaccttcgg
    18661 aacatgctgg gcgctattgc tatgagcgcc gcaagtctca ccaacatcca gtgtgacgag
    18721 gtagttcagc aggccatcgc gcgggacgcc cggcgcattc aacgtaacac ggcccgcatg
    18781 gcacgccttg tcggggattt ggtggacatc gtcagcattg aagccggaca cttggccgtc
    18841 gccccccgtc gtcacgacgt agtggagctc ctgcgtgaga cgatggaagt attccaaccc
    18901 ctcgcggccg ccaagcagat ctggattgga actgacgtga aacgaggctc ccttttggcc
    18961 aggtatgatc acgagcgcgt tcttcaagtt cttgccaacc tggtcggtaa tgccctgaag
    19021 ttcaccgccg aggggggccg cattcacatc ctcgtggagc ccatcgacgc cttcgtccag
    19081 tttgccgtgg ccgacacagg tcgtggaatt gggaccgaac tccccgctat cttcgatcgc
    19141 ttctggcaac tcgcggacaa gagcgcccgg tcagggctgg gacttgggct ctacatttcg
    19201 aagtgtatcg tggaggctca cggaggaaaa atttgggctg agagtcgcgt cggtgaaggc
    19261 agcaccttct actttacact gcccgccgcg gatgctccta gcgtgaccaa tgcaactgcg
    19321 aagtagcaac cgcagcttgg gggggtggca aaatgggcag cgctcttccg accaagcgcg
    19381 gactcgcgtt atcgtttcgg aaccttcgcg cgaccgcgtc ggcaagcgtc tgttcgagtc
    19441 ctcgtcatgt tcgagttcga ggctgggcgc cggcgcggta cggcgcgcag ctcggcgcgc
    19501 ttctgcctgc gccggagggg gcccatcgat cggtcgcgca cgttattcta tggcgagcgc
    19561 aatccagtct cttcggtaat ggcgccacgc tgcaacgagc gcgctattcg tcagagcgcg
    19621 cagccgcgca gtcctcatct cacgcggtcc ccacaacacc gcgccggcgc cctcaacgaa
    19681 cgcacccgcg gttgcatctc ccgtcgtgcc gaatcgctct ccttcttgcc ggatgactag
    19741 cagtgggtcg acgtaacccg cgactcctac cgaatcacga gacgatcacg caccaagccg
    19801 cgcaggctgc gcccgaggga ccatggtcat tttccctgac gacgggggat ccgtgtggcg
    19861 cgcgccagat tcagggttac tcgtgattcc gatgacggca acgaacggtc tcgaatggcg
    19921 cgttgtcttt cgcgtgtcac gcgatggcca acgtctagct acctcggttg tatgcgaagg
    19981 ggcttccgtc gacctcggta gttcaccggc tctcgatatg tggatggcac tcgcaagagt
    20041 acgtcttgcg cacagcgcgt tgggcgtgac ggagaccgag tgtggatgga ttcaccttaa
    20101 cgagctcgcg cagcagcgtg gggttcctga aattcagatc aaggccgaac tctacagagt
    20161 gcgcaagctg ctgatgcgag tcatcgcaga gaaacaatgc ggatggatgc ccatcgagaa
    20221 ccgctatcgc gctcgcctgc tcagaattgg tacgtcggat atcgttgtag agcccggcga
    20281 ctagtgtcct gattgggcca gctgccatcg actgctcgcg gccgcgccgc accgaaccaa
    20341 tgcctcaagt cgacatctcg aattcggcga tgttgccaag tcagtccaat gcgattcgct
    20401 cgtcggaggg tatcggagaa gtctctcgtt cttgattcgg cgatgttcgg ttcggcgcaa
    20461 gcgagagcgc tcatcgttat gctcaacaga agttcttgtg aatagcaacg cctggtggca
    20521 tagtgagg
//
LOCUS       DASTKU010000980         6582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1755430, whole genome shotgun sequence.
ACCESSION   DASTKU010000980 DASTKU010000000
VERSION     DASTKU010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1755430"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="VFQ61_34830"
     CDS             <1..1618
                     /locus_tag="VFQ61_34830"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HET9959737.1"
                     /translation="PFLAFTCLLSLPIFVFTALLFVLFPRVGLSLLLLSHSRPGRMVG
                     FSDKVDLGGVGKLRTDPTIVLRVEVPEVASDPPPRLALYLKGTAFDRYDGRSWSRSQV
                     PNRLPADQRGRAVRILRFPDPARDRKLRIELEPIDPPVVFLPIDAVALTLVTPVNAAP
                     TQVPELFLGPEGGLSYRSLDDRGLRYEVSLATAEDAERFEALAPTEFSRYLALPSNLS
                     PRISELSREWTRGLSEPLDLARALETRLRQNYRYDLDSPAGAAANPLEDFLFVSKRGH
                     CEFYSTAMAVMLRSLGVPSRNVTGFIGGSYNRFARSYAVRQGDAHSWVEAYLPRRGWT
                     RFDPTPPASSAPKSEMRGLAATLRDIVEAAAQRWNRHVINYDLKQQVGLLRSVRDEYR
                     SVMQRAGWFGRLLQSPRWLVGAVLAVVGAYFLIRRLRRRTDKRAEVPAPRKAAELRAR
                     RIVEQYRALELALVARGIPRPSSTPPLAHARALQAAGHPLADEVLALTEQYVRVRFGG
                     EAFEDAHRKRFSERVRAIKQARLEAKATAA"
     gene            complement(1655..2137)
                     /locus_tag="VFQ61_34835"
     CDS             complement(1655..2137)
                     /locus_tag="VFQ61_34835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959738.1"
                     /translation="MASSITSLSHSASVGARRPGVTWMALVAATTLGITGVSRLRAEV
                     SGPAGELSRRREYRLVVQSYPARNVVNGVPLAHARPLASAQRTVTAEQIAEGVAVDVL
                     DMGEAQADSERVIVAWVEPGAADLEMDGHRARPSRGAYVGVADAAPEQARVVLNRKVG
                     "
     gene            complement(2323..3762)
                     /locus_tag="VFQ61_34840"
     CDS             complement(2323..3762)
                     /locus_tag="VFQ61_34840"
                     /inference="COORDINATES: protein motif:HMM:NF042834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6782 family putative metallopeptidase"
                     /protein_id="HET9959739.1"
                     /translation="MRAAVRTLLLGSLVSGSLILACAQPATAPATPARSSSAPSDGAN
                     ASAGGSPNQPVGSAQQAATTREVAEALQLVASVRRLEPRREVPGVRLTRSELLKEMSR
                     MLAQESPPQLMLGTREVLFAADTIAPGFDLEQGIVKMYTSELAGFYDPKVKRMVLISD
                     LSGDAQNITLYHELVHALQDQHFDLTRAFDWQPDMSDQQSALHALAEGDATAAMVEVF
                     AAARGQPAQRLPAELLAAQSLIGQADPALAELPGVMLRSMLAPYADGLAFVQALRSAG
                     EDWSKVDNAFREPPQSTEQLLHPLKYLAREAPHLPPVPAAPPGFEELYRDVMGEQGTR
                     LIFEDWAPNKQAAEAASDWDGDRVVVYRRGNQYAVHWHLVFDNEAAASRALVTLARGA
                     ARPELAPDVDARTAAVRPFADAAAAAANARNLSLCQLRGQRGPFAATRHGRHVGVVFG
                     PYERTGTGVRAAGTCPDALSTAHAIARTQ"
     gene            complement(3759..4421)
                     /gene="rpe"
                     /locus_tag="VFQ61_34845"
     CDS             complement(3759..4421)
                     /gene="rpe"
                     /locus_tag="VFQ61_34845"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET9959740.1"
                     /translation="MSIRIAPSILSADFGRLTEETQTAQAVGADWIHVDVMDGRFVPN
                     ITAGPIVVKAVRKATSLTVDVHLMIEEPERYLEAFAEAGADVLSVHAETHGHLQRTLS
                     AIRALGKRAGVVLNPQTPEDAIRYVLGDVDQILVMAVNPGFGGQRFIPAMLPKIRALR
                     EWIDRDNLAIDLEVDGGISTATARDVVQAGARVLVAGTAIFAQPDRAKALADLRAAAN
                     GT"
     gene            4661..5098
                     /locus_tag="VFQ61_34850"
     CDS             4661..5098
                     /locus_tag="VFQ61_34850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9959741.1"
                     /translation="MITRPLGKQDYDHIVQIIDRWWGGPTTALAHPIFFYELGSMARV
                     VEHDGILVGFLLGFVAPGPPKTGYVHLVGIHPDYRRRGVGKVLYQTFEEDSRAAGCTR
                     LKAITTLGNEGSIAFHKALGWAVTFVEDYAGPARPRVVFNKNL"
     gene            5169..5690
                     /locus_tag="VFQ61_34855"
     CDS             5169..5690
                     /locus_tag="VFQ61_34855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET9959742.1"
                     /translation="MITSLNVEPPLTHTELKNIGLFGGLNDQVMGYLAANLRVEAPEP
                     GQTLFREGEAATAMYVVISGEVEVLKRSRHGIEARVAVLGPNDWFGEMSIVDIQPRSA
                     TVRALAACRLARITPQDLDGLYRHDVRSYAIVILNLARELSRRLRVADGIVADLIANV
                     LDHYVKRPSGGGS"
     gene            complement(5793..>6582)
                     /locus_tag="VFQ61_34860"
     CDS             complement(5793..>6582)
                     /locus_tag="VFQ61_34860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959743.1"
                     /translation="PNGRPENCYTSPRMRASSRALMLAALLGGACMLNPQPDPPAGDG
                     SRSSNDPAPATGGNMNTEIPPTMGGAPSLPPTSASGGASSGTTSTIPLPGLSGAPGTD
                     TPSASGGTGTQAGSGAAAGASAMGGSAATGSFAGGSPGGGFAGVPGAGGAFNSGGGAT
                     IPPSGGSAGGGGAAGGGLPQGGRTSTGGSGTVGESGAGGEGEDPEGGSGSTGAVTGML
                     GTAGTGGNATGGASNSGAAPGGSSSSGQAGHSGTNTGGRLHVEL"
BASE COUNT         1087 a         2181 c         2110 g         1204 t
ORIGIN      
        1 gcccttccta gcgttcacct gcctgctctc gctgcccatc ttcgtcttca cggcgctctt
       61 gttcgtgttg ttcccccgcg tgggcctatc tttgctgttg ctgagccact cacggccggg
      121 gcgcatggtg ggcttctccg acaaggtgga tttgggcggc gtcggtaagc tgcgcacgga
      181 tccgacgatc gtgctgcgag tggaggtgcc tgaagtggcg tccgaccctc caccgcgttt
      241 ggcgctgtac ctcaaaggca ccgcattcga tcgctacgat ggtcgcagct ggtctcgcag
      301 tcaggtcccc aatcggttgc cagcggacca acgcggccgc gccgttcgca tcctgcgctt
      361 ccccgatccg gcccgcgatc gaaagctgcg catcgagctc gagccgatcg atccgcccgt
      421 ggttttcctg ccgatcgatg cggtcgcgct cacgttggtt acgccggtca atgcggcccc
      481 gactcaagtg cccgagttgt tcttgggccc ggaaggcggt ctcagctacc ggtccctcga
      541 cgatcggggc ttgcgctacg aggtatcgct agcgacggcg gaggacgcgg agcgcttcga
      601 ggctttggcg ccgaccgagt tctcacgcta tctcgcgttg ccctccaatc tgtctccccg
      661 catctcggag ctctcgcgcg agtggacccg gggcctctcc gagccgctcg atttggcccg
      721 cgcgctcgag acacgcctgc ggcagaacta ccgctacgac ttggactcgc cggcaggggc
      781 ggccgcgaac ccgctcgaag acttcttgtt cgtcagcaag cgcggtcatt gcgagttcta
      841 ctccaccgcg atggccgtaa tgctgcgctc gctcggtgta cccagtcgca acgtgaccgg
      901 cttcattggc ggcagttaca accggttcgc gcgaagttac gccgttcggc agggcgacgc
      961 acactcttgg gtcgaggctt atctgccgcg gcgcgggtgg actcgcttcg atccaacccc
     1021 acctgcgagc tcggccccca agagcgagat gcgcgggctc gccgccacgc tgcgcgacat
     1081 cgtcgaagct gcggcgcaac gctggaatcg ccacgtcatc aactatgatc tgaagcaaca
     1141 ggtgggcttg ttgcgttcgg tgcgcgacga gtaccgcagc gtgatgcagc gcgcgggttg
     1201 gttcgggcgc ttgcttcagt ccccgcgttg gctggtgggt gcggttttgg ccgtcgtcgg
     1261 cgcgtatttc ttgattcgcc ggctgcgccg acgaacggac aagcgcgccg aggttccggc
     1321 gccacgtaag gcggccgagc ttcgcgccag gcggatcgtg gagcagtatc gagcgctcga
     1381 gctcgctctg gttgcgcgtg ggatcccgcg cccgagcagc acgccaccgc tcgctcacgc
     1441 gcgggcactg caagcggccg ggcacccgtt ggcggacgag gtgctggcat tgaccgaaca
     1501 gtacgttcgc gtccgtttcg gtggtgaagc cttcgaagac gcgcaccgca agcgtttttc
     1561 ggaacgcgtg cgagccatca aacaagctcg cctagaagcc aaagcgacgg ccgcctgagc
     1621 ttctcgacgc gtgccgcgaa cgatcgacgg cgcgtcagcc taccttacga ttcaggacca
     1681 ctcgggcttg ttcaggagcg gcgtcggcca ccccaacgta ggcaccgcgg ctgggacggg
     1741 cacgatggcc gtccatctcc aagtcggccg caccgggctc gacccaagca acgatcacgc
     1801 gctcggagtc agcttgggcc tctcccatat ccagcacatc gaccgcgacg ccttccgcga
     1861 tttgctccgc cgttaccgtg cgctgcgcgg acgcaagcgg ccttgcgtgt gccagcggta
     1921 cgccattgac gacgttgcgc gccgggtagg actgcaccac caatcgatac tcgcgtcttc
     1981 tggagagctc acccgccggt ccggacactt cggcgcgcaa gcgcgaaaca ccagtgatcc
     2041 ccagggtagt ggcagcgacc agcgccatcc aagtcacacc cggacggcgc gccccaacgg
     2101 aagcagagtg cgagagggaa gtgatcgagg aagccatgtt caggtgaccc gctattgacg
     2161 gcgttcgagc gagtgcccga cggtcgccgt tccttgcgtt cacctacaaa gcaatctgtg
     2221 tgccgctcca ttcccaccca aggagtccca cctaccgaat ccccaatggc tcgaacgacg
     2281 agctgatgtt tcgcgagttg cccaggaaag agcggcgcgc catcactgtg tccgggcaat
     2341 ggcgtgcgcc gtactcagcg cgtccgggca ggtgcccgcc gcgcggacgc ctgtcccggt
     2401 gcgttcgtac gggccaaaga cgacgcccac atgacggcca tgtcgcgttg ccgcgaatgg
     2461 cccacgctgg ccgcggagtt ggcacaacga aaggtttctt gcgttcgcgg cggcggcggc
     2521 tgcgtctgcg aacggccgca ccgctgctgt gcgcgcatcc acgtccgggg ccaactccgg
     2581 gcgtgctgct ccccgcgcca aagtgacgag cgcacggctg gcggccgcct cgttgtcgaa
     2641 caccagatgc caatgcaccg catactggtt gcctcttcga tacacgacga cgcgatctcc
     2701 gtcccaatca ctcgccgcct ctgcggcttg tttgttaggg gcccagtctt cgaagatcag
     2761 gcgcgtgcct tgttctccca tcacgtcgcg atagagctct tcgaaacctg ggggggcggc
     2821 cggcaccgga ggaagatgag gcgcctcgcg ggcgaggtac ttcagggggt gaagcagttg
     2881 ttcagtagat tgaggaggct cgcggaacgc attgtccact ttgctccagt cctcgccggc
     2941 tgagcgcaac gcctgaacga acgccaagcc gtctgcatag ggagccagca tcgaacgcag
     3001 catcactccc ggcagctcgg caagcgccgg gtccgcctgc ccaatcaggg actgcgccgc
     3061 gagcagttcg gcgggcagac gctgcgcggg ctggcccctg gcggcggcaa agacctcgac
     3121 catcgccgcg gtggcgtccc cctcggcgag cgcgtgaagc gccgactgtt gatcggacat
     3181 atctggttgc cagtcgaagg cgcgggtcag gtcgaaatgc tggtcctgaa gcgcgtgcac
     3241 gagctcgtga tacagggtta tgttctgagc gtctcccgag aggtccgaaa tcagcaccat
     3301 gcgcttgacc ttgggatcgt agaaacccgc cagctcggac gtgtacatct tcacgatgcc
     3361 ctgctcgaga tcgaagcccg gggcgatcgt gtcggccgca aacagcacct cccgagtacc
     3421 cagcatgagc tggggagggc tctcctgcgc gagcatgcga gacatctctt tgagcagttc
     3481 gctgcgcgtc aagcgcacgc ctggcacctc gcggcggggc tcgaggcgtc ggaccgaagc
     3541 aaccaactgc aaggcctcgg cgacctcgcg ggtggtggcc gcctgctgcg cggatcccac
     3601 tggttgattc ggtgagcctc cggcgctcgc gttcgcgcca tcgctcggag cagaagacga
     3661 gcgcgcggga gtggcgggag ccgtcgccgg ttgcgcgcag gcgagtatca atgaaccgga
     3721 gacgagtgaa ccaagcagca atgtgcgcac ggcagccctc atgttccgtt cgcggctgcg
     3781 cgcagatccg cgagagcctt ggcccggtcg ggttgcgcga agatcgccgt gccggccacg
     3841 agcacgcgcg caccggcttg caccacgtca cgcgcggtag ccgtcgagat cccgccgtct
     3901 acttcgagat cgatcgccag gttatcgcga tcgatccact cccgcagagc gcgaatttta
     3961 ggcaacatcg ccgggatgaa gcgctgaccg ccgaatccgg gattcaccgc catcaccaag
     4021 atctgatcca catcacccag cacgtagcga atcgcgtctt caggggtttg cgggttcagc
     4081 accactccgg cgcgcttgcc caaggcccga atcgcgctga gcgtgcgctg caggtgcccg
     4141 tgggtctccg cgtgcacact gagcacgtcg gcgcctgctt cggcgaacgc ttccagataa
     4201 cgctccggct cctcgatcat caggtgcacg tccaccgtta agctcgtagc tttacggacg
     4261 gccttcacca cgatggggcc ggcggtgatg ttgggcacga agcggccgtc catcacatcc
     4321 acgtgaatcc agtctgcgcc gacagcttgg gcggtttgag tttcttcggt caggcggccg
     4381 aagtcggcgc tcagaatcga gggagcaatg cgaatcgaca cgcagggcta ttagcttgga
     4441 tttggttgtg tgccgtagct tgcgtagtga gcctgtcttt ttcgcgaacg gccattggcg
     4501 acggtgcacc aaagcggcta gggcgcgcta gacctggccg agcgtcgggc gctaggccac
     4561 cagccctgac acgctggact tcgcagcatt ccgcggaaga tctcggcaac aagcgtgtta
     4621 ccttcaagct ccgtctcgag ccactcatcg aaagtcagac atgatcacgc ggccgctcgg
     4681 taagcaggac tacgaccata tcgttcagat catcgaccgc tggtggggag gccccacgac
     4741 ggccttggct catccgatct tcttctacga gctcggctcg atggcgcgtg tggtcgagca
     4801 cgacggaatc ttggtcgggt tcttgctggg cttcgtcgca cccggtcccc ccaagactgg
     4861 ctacgttcat ttggtcggca ttcacccgga ctaccgtcgt cgcggcgtcg gcaaggtgtt
     4921 gtatcaaact ttcgaagagg actcgcgtgc ggccgggtgc acccgtctca aggccatcac
     4981 caccctcggg aacgagggtt ccatcgcgtt ccacaaagca ctagggtggg ccgtcacctt
     5041 cgtggaggat tacgccgggc ccgcgcgtcc gcgtgttgtc ttcaacaaga atctctgatc
     5101 tccgatgacc gggcgaaacg ggcgctgctg cgcccctcgc cggccccgct cctgaatcgg
     5161 gtactgccat gatcacctcg ctcaacgtcg agccgccgct cacccacacc gagctcaaga
     5221 atattgggtt gttcgggggg ctgaatgacc aggtgatggg ctatttggcg gcaaacctca
     5281 gagtggaggc gcccgaacct gggcagacct tgttccgaga gggcgaggcc gcaactgcaa
     5341 tgtacgtggt catcagcggc gaagtagagg tgctcaagcg cagccggcat ggcatcgaag
     5401 cacgcgttgc ggtgcttggt cccaatgact ggttcgggga gatgagtatc gtcgatattc
     5461 aaccccgctc tgcgacggtg cgagctctcg cggcgtgccg gttggcgcgc attaccccgc
     5521 aggatttgga cggcttgtac cggcatgatg tgcggtcgta cgcgattgtg attctgaatc
     5581 tcgcgcgtga gctttctcgt cgattgcgcg ttgcggacgg tatcgtcgcg gatttgattg
     5641 cgaacgttct cgatcactac gtgaagcgcc cgagtggcgg aggcagctag acggtcgcaa
     5701 tcgcggcttt cacgctctag cttctctttc gacgagtgcg aaatgagccg gggtcgcgac
     5761 tcgggccgta cggttctcgc gagagcggct cgtcagagtt cgacgtgcag gcgaccaccg
     5821 gtattcgtac cggaatgccc agcttgaccg ctgctcgaag agccaccagg cgccgcaccc
     5881 gagttcgatg cgccaccggt tgcgttgcct cccgtgccgg cggtgcccag catgccggtc
     5941 actgcgcccg tcgagcccga gccgccttcc ggatcttctc cttcaccacc cgcaccggac
     6001 tcgcccaccg ttccgctgcc gccggtgctc gtgcgaccac cttgcggcaa gccaccaccg
     6061 gctgcgcctc cacctccggc agagccaccg ctcggcggga tcgtagcgcc cccgccgcta
     6121 ttgaaagcgc cgcctgctcc gggtacgccg gcaaagccgc cgccgggcga accacctgcg
     6181 aagctaccgg tagcggcgga acctcccatc gcgctcgctc ccgccgccgc tccgctaccg
     6241 gcttgcgtgc ccgtgccgcc gctcgcgctc ggcgtatcgg tacccggcgc tcccgagagc
     6301 ccaggcagag gaatggtcga agtagtgccg ctgctcgcgc cgccgctcgc cgaggtgggg
     6361 ggcaaagacg gagcaccgcc catggtgggc gggatctccg tattcatgtt gccgcccgtc
     6421 gccggtgcgg gatcgttgct gctcctgctg ccatcacccg ctggaggatc cggttgcggg
     6481 ttcaacatgc aggcgccccc aagcagggcc gcgagcatta gggcacgaga ggacgcacgc
     6541 atccgaggcg aggtgtagca gttttcgggc cgtccattcg gt
//
LOCUS       DASTKU010000981         5909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1309613, whole genome shotgun sequence.
ACCESSION   DASTKU010000981 DASTKU010000000
VERSION     DASTKU010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309613"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VFQ61_34865"
     CDS             complement(<1..1043)
                     /locus_tag="VFQ61_34865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959744.1"
                     /translation="MRLRTLHRALLGFAFGPLLGCGGDTSPRKGQIMLALQTDMNIPT
                     DVSHVRIVVLKNGALKFDQKYRVGKDGEEIPATLGIIASDNASDTTEVRVLSYQGSKV
                     RTLNRVVTTIPTNRVATLNVPIQWLCDGQVKSSDAAADGFDSSCDDPEAACIAGECRK
                     VYVDSVTLPDFELADVTGGSAVPEEGLCYDTAACLDLGRDITPDAQCQIAVPKKLLPK
                     LNVGLRLPKDGGGICGDAGNCYIPLDSNPDSGFRVVDGSKDPAIVALPKAACTKLASG
                     DAAAVRVSDACVTKTPSIPTCGPWTSVGTRNPNKGPTAGSGASPGVEPGTGGAGGAGG
                     EGTDATGGTVDSHT"
     gene            complement(1040..2116)
                     /locus_tag="VFQ61_34870"
     CDS             complement(1040..2116)
                     /locus_tag="VFQ61_34870"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9959745.1"
                     /translation="MTRIHHRSLLVPPAARRVIATVTLLCVSAQAFAQTTAASPEKPV
                     PSDKAATPSAPPTANLPLSESLKGMARAEYEAGKLLYADGDFAGAALKFERAYEEGKD
                     ARLLWNIAAAEKNRRKYVRVYNLIERYLAEANAALSDADREEARNLLDTVKGFIGELT
                     LEVSPEGAVVDVDDQAAGIAPFTKPLRVEMGERRFRIHKPGFTDVVRTETVPGGGAMT
                     LQVQLVPEVHEGKLRILAGAGDEIRVDDRLLGVSHWEGPMPSGIHRVQVSARGKRPYT
                     SDVGVTDNQTASLRVTLESSAPVSPIREGGGMKAWPWIAGGVAAVGLGVGAYFLFRPR
                     ETQQTPPIDGTLDPGSIPLALRFQ"
     gene            complement(2201..3775)
                     /locus_tag="VFQ61_34875"
     CDS             complement(2201..3775)
                     /locus_tag="VFQ61_34875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9959746.1"
                     /translation="MALSAAEGPPVAPGDVLAGKYRVERVLGAGGMGVVVAALHLELD
                     ERVAVKFLLPEVSQDSNAAARFIREARAAARIKSEHVARVIDVGRLESGAPYMVMEYL
                     EGSDLESLVSQGPLPIEDAVDYVIQACHAMAEAHGVGIVHRDLKPANLFVCQRPDGTR
                     GVKVLDFGISKTTDTGEANLTRTSTMMGSPLYMSPEQMRSARDVDRRTDIWALGAILY
                     QLFSGRTPFHGETLPELVSAILTDDPAPLCNARPELPRELEAIVMRCLRKTPSARFDH
                     VGELVHALTPFGSRRSRYTAERVAISFGTEPAAASLAPKVPELQATLGVGGQTATAWA
                     ETSGEERRHLNRRKWLILGSAGLGVVAAFVLLLSPLLRSHASEPAPQTSRSSSTVLRA
                     DSAPLLEADSTRRPASTTAKNESGTSNAEANSNSPSNAQPAPDITPMPAGASTVTPNS
                     TGASTNAGNTSPPSNVAVKPAAGAPAGATRSNGARAPSTKPAPVVAKAPEVVAPKPPA
                     PAEPAPKKNPLAIELK"
     gene            complement(4025..5302)
                     /gene="serS"
                     /locus_tag="VFQ61_34880"
     CDS             complement(4025..5302)
                     /gene="serS"
                     /locus_tag="VFQ61_34880"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET9959747.1"
                     /translation="MLDLKFIRENPDILRAAIRDKHVALDLDRLLALDQEVTALQRSL
                     QGLQEKRNQNAKQIPKAAAEERQGLVELGRKIGDEIALLKPSIEQSQRELSDLLLLVP
                     NVPAEGAPRGASEADNVRVKSWGAPPEFKFTPRDHVELLRVNGWYELDRIAKVAGSRS
                     YALKGTAVLLEQSLLRFALDTLVARGFTPMSVPAFATEAAFLGTGHFPTGREQVYHIE
                     LDDKFLTGTSEVTLNTLHSGEILNEADLPVLYAGISSCFRREAGSAGKDVRGLIRVHQ
                     FNKVEQYVLCKNDPDESERWHKTLLEVSEGILQALEIPYQVVECCTGDMGLGKYRMFD
                     VESWVPSESKYRETHSCSNLHDWQARRADLRYRDEAGKVRFCHTLNNTAIASPRILVP
                     LLENHQQEDGSIRIPEKLRPYLGGLEFARGPKP"
     gene            5337..>5909
                     /locus_tag="VFQ61_34885"
     CDS             5337..>5909
                     /locus_tag="VFQ61_34885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET9959748.1"
                     /translation="MIEATDETSDSVLRAITDDGAFRVITARTTRTVRGAVRAQSVGG
                     SLARTFGDLLTGAILFRETMAPELRVQGILRGAAGLGSIVADSHPSGKTRGLVQVEGS
                     RELEIETGAVIQMMRTLPSGRINQGVVEVPPDGGVSQALMAYMQTSEQVVSMLAVSTL
                     LEEGEVVAAGGYMVQLLPEVGRGPLMVMTER"
BASE COUNT         1001 a         1918 c         1917 g         1073 t
ORIGIN      
        1 gtatgcgaat cgacggtacc acccgtagca tcggtaccct caccgccggc gccacccgcg
       61 ccccccgttc cgggttccac gcccggtgac gcgccgctgc ccgcggtggg gcccttgttc
      121 gggttcctgg tgcctacgct cgtccatggt ccgcatgtcg ggatcgacgg tgtcttcgta
      181 acgcaagcgt cgctcacgcg cacggccgcg gcgtccccgc tcgcgagctt ggtgcaagcg
      241 gccttgggca aggccacgat cgcgggatcc ttcgaaccat cgacgacgcg aaagcccgag
      301 tcgggattgc tatcgagtgg gatgtagcaa ttgcctgcgt cgccacagat gcctccgcca
      361 tctttgggca gccgcaatcc gacattcagt ttggggagca gcttcttcgg aactgcaatt
      421 tggcattgcg cgtccggggt gatgtcgcga ccgagatcga gacacgccgc cgtgtcgtaa
      481 cacagacctt cttcgggaac ggccgagcca ccggtgacgt ccgcgagctc gaaatccgga
      541 agcgtaaccg agtccacgta caccttgcgg cactcaccgg cgatgcaggc ggcctcggga
      601 tcgtcacagg atgaatcgaa gccgtccgcc gccgcgtcgc tcgatttgac ctgtccgtca
      661 cacaaccatt ggatcgggac gttcagggtc gcgactcgat tggtcgggat agtcgtcacg
      721 acgcgattca gggtgcgaac cttcgaacct tgataagaca gcactcgcac ttcggtggtg
      781 tccgaggcgt tgtcactggc gatgatcccg agcgtagccg ggatctcctc gccgtcctta
      841 ccgacgcgat acttttgatc gaacttcagc gcaccgttct tcagcacgac gatgcgcacg
      901 tggctcacgt cggttgggat attcatgtcc gtctgcaagg ccaacatgat ttggcctttg
      961 cgcggcgaag tgtctccacc gcagcccagc agcggcccga aagcaaaccc gagtagcgct
     1021 cgatgtagcg tgcgaagtct cattggaacc tcaaagcaag cggaatggat ccgggatcga
     1081 gggtgccgtc gatcggcggg gtctgttgag tctcacgagg gcggaacaag aagtaggcgc
     1141 cgacaccgag ccccacggcc gccacgccac ccgcgatcca cggccaagcc ttcatgcctc
     1201 ctccctcccg tatgggggac accggcgcag agctctcgag ggtcacgcgc agactcgccg
     1261 tctgattgtc ggtgacacca acgtccgacg tgtaaggacg tttgccgcgc gcgctgacct
     1321 gcacgcgatg gatcccgctc ggcatcggcc cctcccaatg gctcacgccc agcagacgat
     1381 catcgacacg gatttcgtcg ccagcgcctg ccaatatgcg cagtttgcct tcgtgcacct
     1441 cggggacgag ttggacctgc aatgtcatgg ccccaccgcc gggcacggtc tcggtgcgca
     1501 ccacgtcagt gaatccaggc ttatgaatcc gaaagcgccg ctcacccatc tccacgcgca
     1561 gcggcttggt gaacggcgca atgcccgcgg cctgatcatc gacgtcgacg accgctccct
     1621 cggggctgac ttcgagggtc agctccccaa tgaagccttt gacggtgtcc agcaggttgc
     1681 gcgcttcctc gcggtcggcg tcactgagcg cggcattcgc ttccgcgagg taacgttcga
     1741 tcaagttgta aacgcgcacg tacttacggc ggttcttctc cgcggcggcg atgttccaga
     1801 gcaaccgcgc gtctttgccc tcttcgtagg cgcgctcgaa tttcagcgcg gcgcccgcga
     1861 agtcgccgtc cgcgtagagc aacttgcccg cttcgtactc ggcccgagcc atgcccttga
     1921 ggctctcgga gagcggcagg ttcgccgtcg gcggcgcact cggggttgcg gccttgtcgg
     1981 acggaaccgg cttctcgggg gaagcggccg tcgtttgcgc gaacgcctgc gcgctcacgc
     2041 acagtaacgt gaccgtagcg atcactcggc gcgcggcggg cgggacaagc aaagatcgat
     2101 gatgaattcg cgtcatgagt ctcctcgact ggttcctcgc ggcgggcttt gccttcgtcg
     2161 ttcgcagttc agactttcgg aaggtttgcc aaagacgtgc tcacttgagt tcgatcgcga
     2221 gcgggttctt cttcggtgca ggctcggcgg gtgccggcgg cttgggagca acgacctctg
     2281 gcgccttcgc cactaccgga gcgggcttgg tgctcggagc gcgagccccg ttcgatctgg
     2341 tggcgccggc gggcgcgccc gctgcgggct tcaccgcgac gttgctgggc ggagaagtgt
     2401 tcccggcatt cgtgctcgca ccggtactgt tcggggtaac cgtgctggcg ccagccggca
     2461 tgggcgtgat gtcgggggcg ggttgggcat ttgacgggga attggagttc gcctcggcgt
     2521 tcgaagtacc gctctcgttc ttggccgtgg tcgacgcggg tcgtctcgtc gaatcagctt
     2581 cgagcaacgg agcagaatca gcgcgcagca cggtcgacga ggaacgcgat gtctgcggcg
     2641 ccggctcgga agcgtgggat cgcagcaacg gactcaacag caacacaaaa gccgccacca
     2701 cgccgagccc cgcgctgccg aggattagcc acttgcgccg gttcaggtgg cgccgctctt
     2761 cgccggaggt ctccgcccaa gccgtcgcgg tctgcccacc cactcccagt gtcgcctgca
     2821 gctccggcac cttgggcgca agcgaggccg cggccggctc cgttccgaac gagatggcca
     2881 cccgctccgc ggtgtaacgg ctgcgtcgcg agccaaacgg cgtcaacgcg tgcaccaact
     2941 caccgacgtg gtcgaagcgc gcgctcgggg tcttgcgcag gcagcgcatc acgatcgctt
     3001 cgagctctcg gggcagctcg gggcgagcat tgcagagtgg cgctggatca tcggtcaaga
     3061 tggccgagac gagctcgggg agggtctcgc cgtggaacgg tgttcggccg gagaacaact
     3121 gatagaggat cgcgccgaga gcccaaatgt ccgtgcgccg gtcgacgtcc cgcgccgagc
     3181 gcatctgctc gggagacatg tagagtggcg agcccatcat ggtgctcgtg cgggtcagat
     3241 tcgcttcgcc agtgtcggtc gtcttggaga ttccgaagtc caggaccttc acgccgcgag
     3301 tgccgtccgg gcgctggcag acgaagagat tggcgggctt gagatcgcgg tgcacgatgc
     3361 ccacgccgtg ggcttcggcc atcgcgtgac aggcctggat gacgtaatcg acggcgtcct
     3421 cgattggtag cggtccctgg ctgacgagcg actccagatc ggagccctcc aggtactcca
     3481 tcaccatgta cggtgcgccg ctctccaggc gccccacatc gatgacccgg gccacgtgct
     3541 cgctcttgat gcgcgcggcc gcgcgcgctt cccgaatgaa acgcgcggcc gcgttggagt
     3601 cctgactcac ttcgggcagc aggaacttaa cggctacgcg ctcgtccagc tccaggtgga
     3661 gcgcggcgac gacgaccccc atgccacccg cgccgagcac gcgctcgacc cggtattttc
     3721 cggccaaaac gtccccgggg gcgacaggcg ggccttcggc tgcagacagg gccatgccac
     3781 gcgggtaacg gcagccagaa cgacacgcaa aaggggcgtc gctcgcatcc ccccgccgct
     3841 caccttgcga ccgggcagca cgaggccgcc cgttgcacga caagccccgc gtcgcgctcc
     3901 gtcagacgca caggcggagt tcgacaggct gcaacgcgcg tgatgctcca cgtcgagagc
     3961 ctcggttcga gtgctctcga cgccagcacg ccgtgactcg tcgccactac gcgcgatcga
     4021 ccccttacgg tttcgggccg cgcgcgaact cgagcccacc caagtagggg cggagcttct
     4081 ccgggattcg aatgctcccg tcctcttgct gatgattctc cagcagcgga accaggatgc
     4141 gcggcgacgc aatcgcggtg ttgttgagcg tgtgacagaa gcgcaccttg cccgcctcgt
     4201 cgcggtaacg caggtctgcc cgccgcgcct gccaatcgtg caggttggaa cagctgtggg
     4261 tttcgcggta tttggactcg ctcggcaccc aagactccac gtcgaacatt cgatatttgc
     4321 cgagccccat atcgcccgtg caacactcga cgacctggta cggaatctcg agcgcttgca
     4381 agatgccctc ggagacctcg aggagcgtct tgtgccaacg ctcgctctcg tccggatcgt
     4441 tcttgcacag cacgtactgc tcgaccttgt tgaactggtg cacccggatc aagccgcgca
     4501 cgtctttgcc ggcactgccc gcctcgcgcc gaaaacagct cgagatgccg gcgtagagca
     4561 ccggcagatc cgcctcgttg aggatctccc cagagtgcag ggtgttcagc gtgacctcgg
     4621 acgttccggt caggaatttg tcgtcgagct cgatgtgata gacctgctct cgcccggtgg
     4681 ggaaatgccc ggtaccgagg aatgccgcct cggtcgcgaa ggccggcaca ctcatggggg
     4741 tgaagccgcg cgccaccaag gtatccagcg cgaagcgcag cagactctgc tccagtagca
     4801 ccgcggtgcc tttcagcgcg taggagcggc tgccggcaac tttagcgatg cgatcgagct
     4861 cgtaccaacc gttgacgcgc agcaactcga cgtgatcacg cggggtaaat ttgaactcgg
     4921 gcggcgcgcc ccaagatttc acccgcacgt tgtctgcttc tgacgcgccg cgcggcgcgc
     4981 cctccgcggg gacattggga acgagcagca gcaagtcgct caattctcgt tggctttgct
     5041 cgatgctggg cttgagcagt gcgatctcgt ccccgatctt gcgtccgagt tcgaccagcc
     5101 cctgacgctc ctccgcggcc gctttgggga tctgcttggc gttctggtta cgcttctcct
     5161 gcaacccttg cagagagcgc tgcaaggccg tgacctcttg gtcgagcgcg agcaagcggt
     5221 cgaggtctag ggcgacgtgc ttgtcgcgga tggcagcgcg aaggatgtcc ggattttctc
     5281 ggatgaattt taggtcgagc atggtcagaa gccgggccag agcgtatcct ccaacggtga
     5341 ttgaagcaac ggacgaaaca tcggatagcg tgctccgcgc catcacggac gacggcgcat
     5401 ttcgggtgat caccgcgcga accacacgga cggtacgcgg cgcggtgcgg gcccaatccg
     5461 taggcggcag cctggcgcgc accttcggcg atctgttgac gggcgcgatc ctgtttcggg
     5521 aaacgatggc gccggagctg cgcgtccagg gcattctgcg cggcgcggca ggcctgggca
     5581 gcatcgtggc cgactcgcat ccatccggca aaacacgcgg gctggttcag gtcgagggca
     5641 gccgtgagct cgaaatcgag accggcgcgg tgatccagat gatgcggacc ttgccgagtg
     5701 gccgaatcaa tcagggagtc gtcgaggtac ctcccgacgg cggggtctcc caggcgttga
     5761 tggcctatat gcaaacctcc gaacaggtcg tgtcgatgct agcggtcagc acgttgctcg
     5821 aagagggcga agtcgtggcc gcgggcggtt acatggttca acttctgcca gaggtcgggc
     5881 gcgggccgct gatggtgatg accgagcgt
//
LOCUS       DASTKU010000982         8977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_413998, whole genome shotgun sequence.
ACCESSION   DASTKU010000982 DASTKU010000000
VERSION     DASTKU010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8977
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413998"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFQ61_34890"
     CDS             <1..185
                     /locus_tag="VFQ61_34890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959749.1"
                     /translation="ELTPEALLLWSDSGRVAVLRWELESNEREWLSDRLTHYLETENL
                     AAIGEQRDQVDRAEPD"
     gene            complement(132..638)
                     /locus_tag="VFQ61_34895"
     CDS             complement(132..638)
                     /locus_tag="VFQ61_34895"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HET9959750.1"
                     /translation="MPPSKTNAPRSAGQLRLPAWLPALVRLLDSAFEIPGTRLRVGLD
                     PILGLIAPGAGDAVGGIAALSVLWVAFTRRVPKVVLLRMLVNLIVDALIGVIPVLGDI
                     FDVAYRANQKNLELLQRHAGQAHPKPTLGDYAIVGLAFACVLALLALPILIGLGAVHL
                     IALLTDGS"
     gene            780..1226
                     /gene="dtd"
                     /locus_tag="VFQ61_34900"
     CDS             780..1226
                     /gene="dtd"
                     /locus_tag="VFQ61_34900"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET9959751.1"
                     /translation="MRALVQRVRRAQVQVAGEVVARVEHGLCVFVGIASSDGDAQAIW
                     LSDKLLGLRIFEDPAGKMNLSVREVGGALLLVSQFTLYGDTRRGRRPSFTEAMRPEGA
                     EPLFEQVVAAVRQSGLTVATGRFRTDMQVELVNDGPVTLWIDSALK"
     gene            complement(1294..3600)
                     /locus_tag="VFQ61_34905"
     CDS             complement(1294..3600)
                     /locus_tag="VFQ61_34905"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9959752.1"
                     /translation="MQETQDPFGWLGATLDGKYRIDQVVGQGGFGLVYRAYHLGFNET
                     VAVKCLRLPQSMNAQERERFRETFLAEGRLLHQLSRATAGIVQALDIGAAVSPSKIYT
                     PYLVLEWLEGRPLDRDFAERRESKLGGRSVLESYELLNPAVRALQVAHTQGVAHRDIK
                     PANLFLATVAGKQVTKVLDFGIAKVMSDSESLSRAFEETGATMLAFTARYGAPEQFSR
                     RYGATGPWTDVFALALVFEEAIAGRPALEGTDAAQLFVSAVDPERRPTLRKLGLECSD
                     ALDNVLRKALAVDPRERYAEASAFWAALESAVKEPEPVFPESAYAVTAPGPDSLPASG
                     SPASGNPASANPASNARAVPATSAPVSPGTLPHSAAAAIPAPASAPAASLPVPSSPRT
                     ELASARAIPKTGRSRFKVLLWVSGLALLTGVGITITAARMLRPISSRIPGPASTDARN
                     EEPNAGPRNEDGAGPAPDAQTPTPNATSPASAAGSEPSPEVPPLAEPAPSAVQNATPP
                     PGAQVGPLFVWPEQEVPAGPIGSEAFIGAYKTWSEQTNGLSFGQAARQCATHGLAICT
                     ESQWLHACRHLPALAKVNTWTSSVKGPNVVVHEGETCEGRSEASPLSAAPERAALCCG
                     RAIAMTTDNLQKQYLTTTAERVLLIERGINQHDTKSLLELSDVQISVDDHTKTNAQFG
                     KLLENNFQAAPELNIITDTCEVSVRAKRITKTRPRRKKQVTYQTQGWVAECEQTSISG
                     ERVDAFHVTYEFTAQSKLRALSTEPSDP"
     gene            3715..4023
                     /locus_tag="VFQ61_34910"
     CDS             3715..4023
                     /locus_tag="VFQ61_34910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET9959753.1"
                     /translation="MPTIEILANKIGRKKLVESARGGELVDICDEHLAPIPFSCRSAS
                     CGTCEVEVLEGQELLEPPGPVEAELLQVLRAAPSHRLACQARVKDGPGMVRLRAVLAG
                     "
     gene            complement(4038..5033)
                     /locus_tag="VFQ61_34915"
     CDS             complement(4038..5033)
                     /locus_tag="VFQ61_34915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HET9959754.1"
                     /translation="MLALASSSRSEPLELRTVAEPKLGPHSVLIEVHAAGINRADLLQ
                     RRGLYPPPPGESEILGLECSGVVRSVGREVQSVREGERVMALLAGGAYAELVAVHESL
                     VLPVPARLPLSAAAAVPEAFLTAYEALFRQADLKPGERVLIHAGASGVGSAAIQIARE
                     LGAFVFATTRGEKLERLETLGAERPIDYQTEDFAKVIEEVSRGNGVDVILDLIGGAYA
                     ERNQACLARGGRWISVGLLGGARANIDLGLLLRRCQTLRGMVMRSRPLPEKSAIVQGF
                     RRELWSWLESARIEPIVDRIVPWQEAESAHERMEQNLNVGKIVLAVRGDLPASST"
     gene            complement(5126..6013)
                     /locus_tag="VFQ61_34920"
     CDS             complement(5126..6013)
                     /locus_tag="VFQ61_34920"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET9959755.1"
                     /translation="MLIGVDFGGTHVKAGVVESGEVRKLNTAATAAGSSPREILDVLA
                     RTVLSLSPNPEAVGVAIPGEVDAEGRCWKLTNVPGFAGVHIAAELSQRLGGCPIAVEN
                     DATSAALGERLYGHGREHPSFLMVTLGTGIGSGLVLGHMLYPGSNGFAGEFGHINIDS
                     SSSAPPCACGQRGCVESFVGTRALVRRFQEHGGPVTAEVLPIAEAARRGEPAGLSAFQ
                     MLGEALGKGLATVQNLLDLNAIVFTGGVSAALDLFESHVRLALRIHCFAPPLAEVPLL
                     RSELGERAGIIGAAHLTTL"
     gene            6166..7026
                     /locus_tag="VFQ61_34925"
     CDS             6166..7026
                     /locus_tag="VFQ61_34925"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET9959756.1"
                     /translation="MTTLRLATLNLWNKSGPWPQRLELIQREIRRLKPDLLGLQEVLR
                     LAHPSETNAEHTPRTCQAHEVREGFQYRVAYAKASDFGGGLLFGNALLSRYPILEQQD
                     FVLPGATPEESRVLLYACVETPYGELPVFVTHLNWKLHHASIRLEQVRFVAEQIGKLC
                     PVRGPRLPPVLMGDFNADPDSDEMRFLRGLAVVEGKSVYFADAWIYGGPPEPGATFDR
                     QNAFASRAHEPSRRIDYIFVRGPDRLLRGEPVTTALAFNRPETFPEGRVWPSDHFGLV
                     SDIVFEARSP"
     gene            7161..7472
                     /locus_tag="VFQ61_34930"
     CDS             7161..7472
                     /locus_tag="VFQ61_34930"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HET9959757.1"
                     /translation="MKHVVNHDLGQERAKKVAESALSSYKEKFAKYSPAVNWVTPNKA
                     DIGFNVKGVSLKGALEVSERTIEMELDVPFLLRPFKGQALAVIESEINEWIAKAKSGA
                     L"
     gene            complement(7532..8356)
                     /locus_tag="VFQ61_34935"
     CDS             complement(7532..8356)
                     /locus_tag="VFQ61_34935"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HET9959758.1"
                     /translation="MASCLVAAGILAWAGLAPAPLLPPSIPPGFSGSHAAENDAPPEF
                     VVPPLSRGASTGTSTETFPFQKPLPARLATRTVGGRIARLSLKQCQQELKKRGIAIQP
                     PKRPAPGVSGPVRLAGPLHGVRFTTPGPKVPYGILDCRLVLALDELSKVLDRYEVTGV
                     RIDNFYRPGAKLPGKRKPSQHAMGLAADIMGFTRAGGRDLVFERDFQPTLGAPTCGPE
                     ASSEGLSEAAITLRNIVCDLSREGIFQHLLTPNHDRSHENHLHADIAREAKTLIVK"
     gene            8657..>8977
                     /locus_tag="VFQ61_34940"
     CDS             8657..>8977
                     /locus_tag="VFQ61_34940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959759.1"
                     /translation="MRLIGPLPRPAFIARLGVVHRLFTPLYVLLLVLCARVPWAWGAG
                     SQAKPTAIAPVQVKYWEKWTGLERDAMRAVIDDFNRSQTSIQVEYVAVTAISQKLLVA
                     TAGGN"
BASE COUNT         1627 a         2839 c         2902 g         1609 t
ORIGIN      
        1 cagagctcac gccagaggcg ttgttgcttt ggtctgactc ggggcgggtc gccgtcttac
       61 gctgggagct cgagagcaat gagcgggagt ggttgagtga tcggctcact cattacctgg
      121 agaccgagaa cctagctgcc atcggtgagc agcgcgatca ggtggaccgc gccgagcccg
      181 attaggattg gcaaagccaa taaggccaaa acgcaggcaa acgcgagccc cacgatcgcg
      241 tagtcaccga gggtcggctt cgggtgggcc tgccccgcgt gtcgctggag gagctccagg
      301 ttcttctggt tggctcgata ggctacgtcg aagatatcgc cgagcacggg gatgactccg
      361 atcagagcgt ccacgatcag gttgaccagc atgcgcagca acacgacctt cggaactcga
      421 cgcgtgaatg cgacccaaag caccgaaagc gcggcgattc cgcccaccgc gtcccccgcg
      481 ccgggggcaa tcaagcccag gatcggatcc agacccacgc gcaggcgtgt gccggggatc
      541 tcgaaggcgc tgtcgagcag gcgcaccaag gccggcaacc aagcgggaag gcggagctgg
      601 cctgcagatc gaggggcgtt cgtcttcgaa gggggcaccg atgcgggaag gctcaccgct
      661 ggcagtctgg ttctcgaagc gcggattgcc aactgcggtg cggacttttt ggcaactcgc
      721 tacaccgagc atggttctag ggaagacggc tacgaaagcg tgctaacgct cttctcgaca
      781 tgcgggcgct cgttcaacgg gtcagaagag cccaagtcca ggttgctggc gaagtcgtgg
      841 cgcgcgtcga gcacgggctg tgcgtattcg tcgggattgc gtcgagcgat ggcgacgcgc
      901 aggcaatttg gctttcggac aagctgctcg ggctgcgaat cttcgaggat ccagcaggaa
      961 agatgaacct gtccgtacgc gaggtgggcg gggcgctgct cttggtctcg caattcactt
     1021 tgtacggcga tacgcgacgt gggcgtcgcc cgagcttcac ggaggccatg cgacccgaag
     1081 gcgccgaacc gttgttcgag caggtcgtcg cggcggtgcg gcagagtggg ctgacggtag
     1141 caaccgggcg ttttcgcacc gacatgcagg tagaactcgt gaacgacggc ccggtcacgt
     1201 tgtggatcga ctcagcgctc aagtgagctt cttctggagc gcgaactcgc agctgggaga
     1261 aatcgggagg gaagaggccc gaggacgaac tcttcagggg tcgctgggct cggtggacaa
     1321 tgcccggagc ttgctttgcg cggtaaactc gtaggtgacg tggaaggcgt cgacgcgttc
     1381 gcccgagatg gaggtttgct cacactcggc gacccaaccc tgagtctgat acgtcacctg
     1441 cttcttgcga cgggggcgcg tcttggtgat gcgcttggcg cgcacggaga cttcgcaagt
     1501 gtccgtgatg atgttgagct cgggagcggc ctggaagttg ttttcgagca atttcccgaa
     1561 ttgagcattg gtcttcgtgt gatcatcgac cgagatctga acatcggaga gctccagtag
     1621 gctcttcgtg tcgtgttggt taatcccgcg ctcgatcaac agcacgcgct ccgccgtggt
     1681 cgtgaggtat tgcttctgca ggttgtcggt tgtcatggcg atcgcccgcc cgcaacacag
     1741 cgcggcgcgt tcgggcgcgg ccgagagagg agacgcttcg ctgcgccctt cgcaggtctc
     1801 cccttcgtgg acgaccacgt ttggaccttt gacgctgctg gtccaagtat tcaccttggc
     1861 caatgccggc aagtgcctgc aggcatgaag ccactgcgat tcggtgcaga tcgcgagacc
     1921 gtgggtggcg cattggcgcg cggcctggcc gaagctcaag ccgttggtct gctcgctcca
     1981 tgttttgtac gcgccaatga aggcttcgga gccaatcggc ccagcgggca cctcctgctc
     2041 gggccagacg aacagcggtc ccacctgggc acctggcgga ggagtggcgt tctgaaccgc
     2101 actcggcgcg ggctcggcga gaggggggac ttccgggctg ggttccgaac ctgctgcaga
     2161 cgcgggcgaa gtcgcattgg gggttggcgt ttgcgcgtcc ggagcgggcc ccgcgccatc
     2221 ttcgtttctg ggccccgcgt taggctcttc gtttcgcgca tccgtggagg cgggcccagg
     2281 aatgcgggac gaaatggggc gtagcatgcg cgcggcggtg atagtgatcc cgacgccggt
     2341 cagcagcgcc aatccggaca cccagagcaa caccttgaag cgtgagcgcc ccgtcttggg
     2401 gatggcgcgg gcagacgcga gctcagtgcg tggtgatgac ggcaccggaa gtgacgccgc
     2461 gggcgcggaa gcgggggcag ggatggcggc cgccgcggag tgaggcaggg tgcccggcga
     2521 aaccggcgcg ctcgttgcgg gaacggcgcg cgcatttgat gcagggttcg cagaagcagg
     2581 gtttccagaa gcggggcttc cagatgcagg caagctgtct ggaccggggg cggtgacggc
     2641 gtaagccgac tccgggaaga cgggctcggg ttctttgaca gcagactcga gcgcggccca
     2701 aaaggccgag gcttccgcgt agcgctcgcg cgggtcgacc gcgagcgcct ttctcagcac
     2761 attgtccaaa gcgtcactgc actcgaggcc gagtttgcgc aacgtgggcc tgcgctctgg
     2821 atccaccgcg gagacgaaga gctgggccgc gtccgtgcct tccagggctg ggcggcccgc
     2881 gatcgcttcc tcgaatacca gggccagcgc gaacacgtcc gtccacggcc ccgtcgctcc
     2941 gtagcgccgg ctgaactgtt cgggggcacc gtagcgcgcg gtgaaggcga gcatcgttgc
     3001 gcccgtctcc tcgaatgcgc ggctcaggct ctccgagtcc gacattacct tcgcgatgcc
     3061 gaaatccagg accttggtga cttgtttccc ggcgaccgtc gccaagaaca agttggcggg
     3121 cttgatgtcg cgatgggcca caccctgcgt gtgcgcgacc tgcagcgcac gaacggcggg
     3181 gttcaacaac tcgtaggact cgagaacgga cctgccaccg agtttgcttt cacgtcgctc
     3241 cgcaaagtca cggtccagcg gtcgcccttc caaccactcc aacactagat agggtgtgta
     3301 aatcttgctg ggtgagactg cggctccgat gtccagagcc tggacgatac cggcggttgc
     3361 gcgggacagc tggtgcagca gtcgcccttc ggccagaaac gtctcacgga aacgctctcg
     3421 ttcctgcgcg ttcatacttt gcggcaagcg caggcacttc actgccacgg tttcattgaa
     3481 gccgaggtgg taggcccgat aaaccaagcc aaatcccccc tgacccacga cttgatcaat
     3541 gcggtacttg ccgtcgaggg tggccccgag ccaaccgaag gggtcttggg tttcctgcat
     3601 tcctgggcct tggagaatgg ctgaaagcgg agcaaagctc aaacgcacag cgcatgccag
     3661 cgcggccgga gcattgcgaa cgctcgtacc cggcgctatt cgatcggtcg gatcgtgcca
     3721 acaatcgaaa ttttggcgaa caagattgga cggaaaaagc tggtggagtc cgctcgaggc
     3781 ggagagctcg tcgatatttg tgacgaacac ttggccccca tcccgttttc atgccgttcg
     3841 gcgagctgcg gcacctgcga ggtcgaggtg ctcgaggggc aagaactact ggagccgcct
     3901 gggcccgtcg aggccgagct gcttcaggtg ctgagagccg cgccaagcca ccgcctcgca
     3961 tgtcaggccc gagtcaagga tggtccgggc atggtccgct tgcgcgcggt acttgcggga
     4021 tgaagcgggc ttgcaagcta agtcgagctt gcaggcaggt cgccacgcac ggcgagcacg
     4081 attttgccaa cgttgaggtt ttgctccatg cgctcgtgag ccgactcggc ctcttgccaa
     4141 ggcacgatcc gatcgacgat cggctcgatg cgcgcggact ctagccacga ccagagttct
     4201 ctccgaaaac cttgcacgat ggctgatttc tccggcagtg ggcggctgcg catcaccatt
     4261 ccgcgtaacg tctggcaacg ccggagcagt aaacccaaat cgatgttggc gcgtgcgccg
     4321 cccaacaagc ccaccgagat ccaacgccca ccccgagcca gacaagcctg attgcgctcg
     4381 gcgtaggcgc cgccgatcaa atccaggatg acgtccacgc cgttaccgcg cgagacttcc
     4441 tcgatcacct tggcgaagtc ttcggtttgg tagtcgatcg gtcgctccgc tcccaaggtc
     4501 tcgagccgtt cgagcttctc accgcgggtg gtcgcaaata cgaacgcccc gagctcgcga
     4561 gcgatctgga tcgcggcgct gcccacgccg ctcgcacccg cgtggatcag gacgcgctcg
     4621 cccggcttca agtcggcttg tcgaaacagc gcctcgtaag cggtcaagaa tgcttcaggc
     4681 acggccgccg cggcagaaag cgggagccgc gccggtactg gcagcaccaa gctttcgtgt
     4741 acggccacga gctccgcgta tgctccgccg gccagcagcg ccatgacgcg ctcgccttcc
     4801 cgcacgctct gcacttcacg gcctacgctt cgcactacac ccgagcattc gagccccagg
     4861 atctcgctct cgccgggagg cggtggatac agcccgcggc gctgcagcag atcggcgcga
     4921 ttgatgcccg ccgcatgaac ttcgatgagc accgagtgtg gtccgagctt gggttccgca
     4981 acggttctca gctccaatgg ttcagagcgc gatgaagagg cgagggccaa catggccagc
     5041 tactctagcg cctcatgccc gcactaagcc tggatttcac gtcgaccgcg cccgaacccg
     5101 agcagtgggt gagctaacga tcccttcaca gggtggtcag atgcgctgcg ccaataatcc
     5161 cggcgcgctc cccgagctca ctccgcaaca acggcacctc cgcgagcggc ggcgcgaagc
     5221 agtggatacg cagcgcgagc cgcacgtgcg attcgaacaa atcgagcgcc gcggacacgc
     5281 cacccgtgaa cacgatggca ttcaggtcca gcaggttctg cacggtcgcc agccccttgc
     5341 cgagggcctc gcccagcatc tggaaagcgc tcagcccggc cggctctccg cggcgtgctg
     5401 cctctgcaat gggcagcacc tctgccgtaa cgggcccacc gtgttcttga aagcgacgca
     5461 ccagggcgcg ggtgccgacg aaagactcca cgcaccctcg ctgaccacac gcacaaggcg
     5521 gcgccgagct ggaagaatcg atattgatat gaccgaactc acccgcgaag ccgttcgagc
     5581 cgggatacag catgtgtccg agcacgaggc ccgagccgat gcccgtgccc agcgttacca
     5641 tcaagaagct cgggtgctcc cggccatgac cgtacagccg ttcaccgagt gcggcactcg
     5701 tcgcgtcgtt ttcgaccgcg atggggcagc cgcccagacg ttgggacaac tccgccgcga
     5761 tgtgtacgcc cgcaaaccca ggcacattgg tgagcttcca gcagcgccct tccgcgtcca
     5821 cttcgccggg aatagcaaca ccgacggcct cggggttggg cgacaacgag agcaccgttc
     5881 gcgccagcac gtccaggatt tcgcgagggc tcgagcctgc agcggtcgcg gcggtattga
     5941 gcttccgaac ctcgccactt tcgaccacgc ccgctttgac gtgggtgcct ccgaagtcga
     6001 ccccgatcaa catagatggg gacacaaata gatgacggcg aagtttactg caatgtaggc
     6061 agttatctcc tgcccaccac gccagcaaac ccattggatg gactacgccg cgaggtgaca
     6121 ttcatgggcg gccggccgcg gtcatcggtt atacgtcgga gcgcgatgac cactctgcgc
     6181 ttggcaaccc tgaacctgtg gaacaagtcc ggaccctggc cgcagcgcct cgagctcatt
     6241 caacgcgaaa tcaggcgctt gaagccagac ctgctcgggc ttcaggaggt gcttcgcttg
     6301 gcccatccga gcgaaacgaa tgcagagcac acgccacgca cttgccaggc ccatgaggtg
     6361 cgggagggct ttcaatatcg cgtggcttac gccaaggcct cggacttcgg gggcggtttg
     6421 ctttttggca acgcgctact cagtcgctac ccaattctcg agcagcaaga cttcgtatta
     6481 ccgggggcga ctccggaaga atcgcgcgtg ctcttgtatg cttgcgtgga gaccccctac
     6541 ggcgaactgc ctgttttcgt gacccacctg aattggaagc tgcaccacgc gagcatccga
     6601 ctcgaacagg tgcgcttcgt cgcagagcaa atcggtaagc tgtgtcccgt tcgggggccg
     6661 cgcttgccgc ccgtgctgat gggggacttc aacgccgatc cagattccga cgagatgcgg
     6721 ttcttgcgtg ggttggccgt ggtcgagggc aagagcgtgt atttcgcgga cgcttggatc
     6781 tacggtgggc cgcccgagcc gggggccacc ttcgatcgcc agaatgcgtt cgcgagccgt
     6841 gcccacgagc cctcacgacg catcgattac atcttcgtgc gcggcccgga tcggctactg
     6901 cgtggcgagc cggtcaccac ggcgctcgct ttcaaccggc cggagacctt cccggagggc
     6961 cgcgtgtggc catcggatca tttcggcctc gtctccgaca tcgtgttcga ggcgcgaagc
     7021 ccgtaacact tgggtcctaa aacccgcccc ggcgagagcc ggcccttgcg cgcccctcgc
     7081 tgcaccaggg actgcgcctc tgtaacctcg gcctttcgcc tttgcattcg gctcgcggat
     7141 ttgccaagct cccgccgccc atgaagcacg tcgtcaatca tgacctaggc caggaacgcg
     7201 cgaagaaagt cgcggagtcc gctttgagct cctacaagga gaagttcgcg aagtactcgc
     7261 ctgccgtcaa ctgggtaacg cccaacaagg ccgatatcgg cttcaatgtg aagggggtca
     7321 gcttgaaggg cgcgctcgag gtgtcggagc gcacgatcga aatggagctc gatgttccgt
     7381 ttttattgcg acccttcaag gggcaagcgc tcgccgtgat cgagtccgag atcaacgagt
     7441 ggattgccaa ggcgaagagc ggcgcgctct aatcgagggc gtggttggac gtctaggcct
     7501 ttgcgaggcg tctagctgtc ccgtcgagct cttatttgac gatgagcgtc ttcgcttcgc
     7561 gcgcgatgtc cgcgtgcagg tggttctcgt gagagcgatc gtgattggga gtgagcaggt
     7621 gctgaaagat gccctcgcgc gataggtcgc acacgatgtt ccgcagcgtg atcgccgcct
     7681 cggacaagcc ttcgctcgac gcctcgggac cgcacgtcgg tgcgccgagc gttggctgaa
     7741 aatcgcgttc gaagaccaaa tctcgtccgc ctgcgcgcgt gaaccccatg atgtctgcgg
     7801 cgaggcccat cgcgtgctgg ctcggtttgc gcttccccgg caacttggcc ccaggtcgat
     7861 agaagttatc gattcggaca cccgtcacct cgtaacgatc cagcaccttc gacagctcgt
     7921 cgagcgccaa gaccagtcga caatcgagga tcccgtacgg aaccttcggc cccggcgtag
     7981 tgaaccgcac cccatgaagt ggccccgcga gccgaacggg gccgctcacg cccggcgcgg
     8041 ggcgcttcgg gggctgaatg gcaatcccgc gcttcttcag ctcctgctga cactgcttca
     8101 acgacaagcg agcgatgcgg ccgcccaccg tacgagttgc caggcgtgct ggcagcggct
     8161 tctgaaacgg gaaagtctcg gtgctggtgc cggtcgatgc gccgcgcgac aagggcggca
     8221 cgacgaactc cggcggcgcg tcgttctcgg ctgcgtggct acccgaaaag cctggcggga
     8281 tactcggcgg caggagcggc gctggggcga ggccagccca ggctaaaatg cctgccgcaa
     8341 cgaggcagct cgccaccctg ctccctcggg ccgtacggtg cccgagcatc ttcagtgctc
     8401 gccacggagc aacccagcgc gaccgcgctg aacgtgggtt caactcgcta ggctgaagca
     8461 cgaaaggttt ggtagcacac gttttcgatg cggtcgcccg cggggcctcg gcaggcacgt
     8521 gtcgcgcggg cgtggcggcg ctttggcgcg cgattctcag cacagaaact tgaccgcagc
     8581 tcgcgactat cggctttctc acccgctctt acccagctct gctggccgcc cccaccctga
     8641 ggtcttcgtc ccgatcgtgc gacttatcgg ccccctaccc agacctgctt tcatcgcccg
     8701 gctcggcgtt gtgcatcggc tgttcacgcc gctctatgtc ctcctgctcg tgctctgcgc
     8761 gcgcgtccca tgggcttggg gggctgggtc gcaggcgaaa ccgaccgcga tcgcccccgt
     8821 ccaagtgaag tactgggaga agtggacggg cctagagcgc gacgccatgc gcgccgtgat
     8881 cgacgacttc aatcgctctc aaacgagcat ccaagtcgaa tacgtcgccg tcaccgcgat
     8941 cagccagaaa ctactcgttg ccaccgcggg cggcaat
//
LOCUS       DASTKU010000983        14612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1980750, whole genome shotgun sequence.
ACCESSION   DASTKU010000983 DASTKU010000000
VERSION     DASTKU010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14612
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980750"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFQ61_34945"
     CDS             complement(<1..253)
                     /locus_tag="VFQ61_34945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959760.1"
                     /translation="MFYSLGPGTRIALVVLVAAVLIGLAAWGLWRIGGQVRRYIWRGL
                     AVVCAIGLLGFGWWSLSLPEPRGSYFILWHQVVRVGAVIS"
     gene            complement(314..1996)
                     /locus_tag="VFQ61_34950"
     CDS             complement(314..1996)
                     /locus_tag="VFQ61_34950"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET9959761.1"
                     /translation="MESSEEALVQARREKAEAYRARGRDPFSNDVDALHRVRVHAVRK
                     RAEPALAGPKSELRYDAERLAALALPEVCLFGRVMGRRGFGKASFWRLRDESGEIQLF
                     AKQDVMGAEFAELEAIDIADHVQVRGPLMVTKTGELSIEVRSIRLVTKALRPLPDKWH
                     GLTDTDQRYRHRYVDLIANPEVAVALRARSMVIQGLRDFLDSRDFLEVETPTLHTLIG
                     GAAARPFKTHHNTLDLELFLRVAPELYLKRLLVGGFERVYEIGRCYRNEGISTRHNPE
                     FTMLEFYQAYATYDSLMTLAQELLRHVDAFLQGRLAKLGLDAYAERWRNERAFTLAEP
                     FARVPMRDAVLASLEKAKLEPSVIERLPELARGPQKAEVQVDTRVGDELVAGYKARSE
                     RAARIDWPNLRRALFACGSDGERLFVAYEYLAEPFLAEDYLSADGTKSRPVFIMDYPF
                     EVSPLARKKDRDPTLTDRFELFVHGRELCNAFSELNDPDDQAARFRAQLAAKAKGAEE
                     TMDYDVDYVRALEHGMPPAAGFGLGVDRLTMFLTGQPSIREVIAFPLLRPEE"
     gene            complement(2004..3132)
                     /gene="prfB"
                     /locus_tag="VFQ61_34955"
     CDS             complement(join(2004..3062,3064..3132))
                     /gene="prfB"
                     /locus_tag="VFQ61_34955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156865221.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET9959762.1"
                     /translation="MLSETRGLLQDLQRRVDALRGSLDVPALKREIDELDSVSSAPDF
                     WNDQDRAQAHMRRRAAAVEKLELIESLSRDVNDALELLELAAAENDEGTVAELERQTP
                     ELSKRVRAAELRRMLSGPVDHANAIVSIHPGTGGTDAKDWAEMLLRQYLRWCERHGYK
                     TDIVDYQVGDEAGIDGASFTVAGPNAFGYLRAEIGVHRLVRISPFDGNARRQTSFAAV
                     EVTPDIEDEIDIEVKDTDLEITTMRAGGKGGQNVNKVETAVRLRHLPTGIMIVCRAER
                     SQHQNRAMALKMLKARLYELEQAKREAAQARYEAAKGQIAWGNQIRSYVLQPYQLVKD
                     LRTEWETSDVQAVLDGDLDPFIEAYLLKNADGGNPTILPNN"
     gene            complement(3219..4076)
                     /locus_tag="VFQ61_34960"
     CDS             complement(3219..4076)
                     /locus_tag="VFQ61_34960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959763.1"
                     /translation="MKTAWFVLGVLCPVGCLTEARADSLRVVANLECRASFESKGSGL
                     RWAGMLAVELPLDRIAAPRPSSVAPLLVAAKVSAGKVTDEPRPEDTERADPPSPRERQ
                     TSRPLDWGMALRLAAAALRKRGDVSIAQRLEAMAARARSSAALPELMLRALRSTDESL
                     RLSPSASSAWDFTHTGGAGWVFEARATWKLDRLVFADEELAIERLRAEREAARERLVG
                     SVLKYFSRWLVARARLANPSLEPERLLVLEAELAFCASALDTLTGGAFKAELERRSAR
                     GGAAASEGP"
     gene            complement(4226..6235)
                     /locus_tag="VFQ61_34965"
     CDS             complement(4226..6235)
                     /locus_tag="VFQ61_34965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959764.1"
                     /translation="MTPAKESAGKLLFRAEGLDLGLLAPLNSGRVDKQSLERLSRDDL
                     VARARLVGVERPELLTRVELSDEIVRLTEPDPLAQRRARGWLGVARDLLASVVESGLN
                     MSDAAARIRGDRAEFEARGPAPVATVTLAEIYATQGHVERALAMLEEVLTAEPDHAEA
                     LALRGRLRVVNRREARSRASKTTTVESTAPSTESAATSAESAATDEESAAIDNARSQS
                     AAPSTESAATDEESAATSTERAATNEQSAATNEESVATNEESVATNEESVATNEESVA
                     TNEKSAAAAPSAGRSDVATPEGSTEASRESREEHDGMLADEGADSLEFAPPSELPSWA
                     EPIPPPESMVFESEHLPRVSSSPVATDVGVSTASQPIVLPKAEPFPLGIDESTSGEES
                     ALVPVPQPVVLPTEEPRPEPPSEPPPEPEEGPLSEPEPEPIAEPIAEPIAEPVAEPIA
                     EPIPDELAEDGVIESGPASLQSPRLPNFAELDRPGESLEGSFATAGWEPAEVDSSSAL
                     ASSDGGDVKDEVATLPTEPPARPIEMEEPEPPVSLLVVRTQAPHPVVCWDVSTLEAVA
                     TSDLEIRCVGFTPNADGASRSEDVLGLDGPRGSRVLDHVGGATRIRVAMFRRSAAAPL
                     AIATEIGWGAAGLEVRFRPPLSQTRPLSAAERALLFDYSRGAASP"
     gene            complement(6207..7307)
                     /locus_tag="VFQ61_34970"
     CDS             complement(6207..7307)
                     /locus_tag="VFQ61_34970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959765.1"
                     /translation="MRAIRNWVLGLTAISMSVACGSASGASLFERRSDEEGLGQGFGG
                     AEIRGSTESSPGTAISGRDAGVLGRGGSSSGVGEGGRDGTSAMMHPGATGGVVGAGSG
                     TGVASAGLGAAGNSDSNSAGSNSGGAPSGGADGLGGAGSSAQAGGSGTAPSTEPACPG
                     GRCVFDCTAEGTCPSPVECPDDRECIVTCGNEACRSGVVCPKGANCTVQCLGTRSCSG
                     KIRCQGEQCNVECTGEKACSGGVDGAASEMQVRCSGPGTCANGVSCGGNVCGIECTGA
                     GACAGDVSANAASDRITCQGQGSCAKQTTCSANDCKVLCSGTGACQGQVTCNASDCSV
                     SCGDCACSSNVYCNAGHASCASGNDAGKGVCG"
     gene            8429..8899
                     /locus_tag="VFQ61_34975"
     CDS             8429..8899
                     /locus_tag="VFQ61_34975"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9959766.1"
                     /translation="MKTPVKTVSPFMEAAQALQFAEDTGVHHLPLVEHGQVRGLVCTC
                     DLEELELRAPISRAVRRPPVTVSTSASIADAMATMAREAVGSVLVLEGQSIVGIVTRE
                     DLNGIDWGDVPGFRCSSCGSVKHLKSEGSRGTLCLDCRSHAGPETPFDETSVGD"
     gene            complement(8949..9962)
                     /locus_tag="VFQ61_34980"
     CDS             complement(8949..9962)
                     /locus_tag="VFQ61_34980"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET9959767.1"
                     /translation="MLPRKIEGSRNVSSDRASLIVIGNFDGVHLGHRTVIQTALAQAE
                     RDGLVPRVLTFEPHPSRVLGGTARPALTLPDRKVELLLRCASDRGLAPDPSEADPNLS
                     VVVEPFTHELSRLSPSEFVESILVQRLHARTVIVGENFRFGRDRVGDLRTLRALGEQL
                     GFDAHAEPLVGDELGVFSSTRARAALELGDLAGVARCLGRPHALSGPVISGAQRGRTL
                     GFPTANLAPVLEALPPFGVYACLVDRVEADGARVLGRGVMNLGMRPTVDAGFSVEVHV
                     LDFAADLYGAELRVHLMARLREERKFEGLAELRAQITLDSERAREALANYAPDPRAGG
                     AWF"
     gene            10070..11143
                     /gene="holA"
                     /locus_tag="VFQ61_34985"
     CDS             10070..11143
                     /gene="holA"
                     /locus_tag="VFQ61_34985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET9959768.1"
                     /translation="MTPDQAVQEARERKLRPVYLVLGDEAHLVRGVVEALRTATLEGA
                     VPGLNEDHMDAAQRSVDDALSAARTLPMLARRRLVLVQRLEHWENRAAAENAEKAADK
                     ARGKGVSVEPFERLLEYAKSPSPSTTLILTGTGIDKRRKLYSAARTDGWLVQCDQLGR
                     AELPGFVEREAKRRSARLGPQIADLLAELLGPELGPVVDAVERLALYAGGEVITEEMV
                     QACVVRLRMATVWELLAAVTRRDLGAALKTLGDVFEPNEAVRLTGLLAWSARQLIRFE
                     AALSAGASPQDAAQQAGAPPFRARELQQQIKSLPRPVLEGWLPILARLDGDLKGGSKL
                     PPQAVLERAIFELCDRRTDARPG"
     gene            11466..12155
                     /gene="ribA"
                     /locus_tag="VFQ61_34990"
     CDS             11466..12155
                     /gene="ribA"
                     /locus_tag="VFQ61_34990"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET9959769.1"
                     /translation="MTHPERSIPRSALETLASAPLPTVHGTFQLRVYRWEDVNAHPAL
                     SREHCALVMGDVRGKRGVGVRVHSECLTSEVFGSLKCDCREQFERAQAEIARRGSGVL
                     LYLRQEGRGIGLANKVRAYALQALGADTIEANELLHLPVDARTYDVAAAILRELGVES
                     IELLTNNPDKVQSLTRLGIQVERRMPVHIAANPFSASYLEVKRRRMLHDLPGVPADAN
                     LTLPTSDPSAE"
     gene            complement(12262..13146)
                     /locus_tag="VFQ61_34995"
     CDS             complement(12262..13146)
                     /locus_tag="VFQ61_34995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET9959770.1"
                     /translation="MIQLGPTLERVAMTQLGSKQERIAGAGAEAPAEQKPPGATEVSG
                     ISTRDLNLWYGDFQALKGITIDIARGQITSLIGPSGCGKTTLLRCFNRVNERYGYVRT
                     TGEIKILGKNIYDPDVSLTELRKSVGMVFQRPNPLPISVYENVVFGMRIHHAKNNLKK
                     AILDEAVHKALSEVGLWDDLKDRLEERATSLQLEQQQKLCIARLLPLKPEIILMDEPC
                     SALDVEGTRAIEELMWALRGQYTIVIVTHNMAQARRASDECVFMLLGELIEKRRTEDL
                     FLNPANHKTAEYIEGRYG"
     gene            complement(13143..13907)
                     /locus_tag="VFQ61_35000"
     CDS             complement(13143..13907)
                     /locus_tag="VFQ61_35000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET9959771.1"
                     /translation="MNPTTPKIRVKDLELRYGDKRVIHGISFDVEEHEIFAIIGPAQS
                     GKSSLLRCLNRTIEFVPGARASGTIQIDGQDVNQMRNVYELRRRVGMVAPLPVGLPLS
                     IYDNVAFAPRAAGIRDKTALDELVQRCLEQAALWDEVKDRLDSLGTKLSGGQQQRLTI
                     ARALSHQPEILCLDEFSIAIDPVTTMRIEDVLKELKRSMTIILVTNLVQQARRLANHT
                     LFLWNGEVMEVDKNDVIFSGRPKNPKTYEYVNGIFG"
     gene            complement(13964..>14612)
                     /gene="pstA"
                     /locus_tag="VFQ61_35005"
     CDS             complement(13964..>14612)
                     /gene="pstA"
                     /locus_tag="VFQ61_35005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET9959772.1"
                     /translation="SLLIAAPVGVLAAVYLNEYAQDNWLTRIINLAVVNLAGVPSIVH
                     ALFGLGAFVLFAGIGRSILAASLTLAVMTLPVIIASTKEALAAVPRTFREACWNVGAT
                     RWQTIRGIVLPNSVSGILTGVILQVSRAAGETAPIMFTGAVFFKKIEEGDPFAYSIWD
                     QCMALSMHLFTITTQVTDAPKALPYATAVVLLGAVLTVNAAAIALRARLRTRKKW"
BASE COUNT         2626 a         4922 c         4449 g         2615 t
ORIGIN      
        1 cgctgatcac cgcacccacg cgcacgacct ggtgccacag aatgaagtag ctgccgcgcg
       61 gctcgggcaa cgacagcgac caccaaccga agccgagcag tccaatcgcg caaacgaccg
      121 caagcccacg ccagatgtag cgacgaacct gaccgccgat gcgccacaag ccccaggcgg
      181 cgagcccaat cagcaccgcc gcgaccaaca cgacgagagc gatgcgcgtc ccgggcccga
      241 gcgagtagaa cacgcgctcg aaccaactgg gtgcgtcagc ggcgatcttc agtcgtgaag
      301 gctgggacag agctcattcc tccggtcgga gcagcgggaa ggcgatcact tcgcgaatcg
      361 agggctgccc cgtcaagaac atggtcagcc gatccacgcc caagccgaac cccgccgcgg
      421 gaggcatgcc gtgctcgagc gcgcgcacgt agtcgacgtc gtagtccatc gtctcctccg
      481 cgcccttcgc cttggccgca agctgagccc ggaaacgggc ggcctgatca tcggggtcat
      541 tcagctcact gaaggcgttg cacagctctc gcccgtgcac gaacaactcg aagcgatcgg
      601 tcaacgtcgg gtcgcgatcc ttcttgcgcg cgagcggtga gacttcgaac gggtaatcca
      661 tgatgaacac cggtcggctc ttggtgccgt cggcgctcaa gtaatcttcg gccaggaacg
      721 gctccgccag atactcgtag gcgacgaaca atcgttcgcc gtcgctgcca cacgcgaaca
      781 acgcgcgccg caggttgggc caatcgatgc gcgcggcacg ctccgagcgc gctttgtagc
      841 ccgcgacgag ttcgtcccca acgcgcgtat ctacctgcac ctcggccttt tgcggccctc
      901 gcgctagctc cggcaagcgc tcgatcacgc tcggctccag cttggctttt tcgagcgagg
      961 cgagcaccgc gtcgcgcatg ggaacgcgcg cgaagggctc cgccagcgta aaggcccgct
     1021 cgttgcgcca gcgctcggcg taggcgtcga gccccagctt ggcgaggcgc ccctgcagga
     1081 aagcgtccac gtgtcgcagc agctcctgcg cgagcgtcat caacgaatcg tacgtggcgt
     1141 acgcttggta gaactccagc atcgtgaact cggggttgtg ccgagtgctg atgccttcgt
     1201 tgcggtagca gcgcccaatt tcgtaaacgc gctcgaaccc gcccaccaac agccgcttga
     1261 ggtagagctc tggcgcgacc cgcaagaaca gctcgagatc gagcgtgttg tggtgtgtct
     1321 tgaatggccg cgccgcggcg ccaccgatca gcgtgtgcag ggtcggagtc tcgacctcga
     1381 ggaagtcgcg agagtccagg aaatcgcgga gcccttggat caccatcgat cgcgcgcgca
     1441 gcgcgaccgc cacctcgggg ttcgcgatca aatcgacata gcggtggcgg tagcgttgat
     1501 cggtgtcggt caggccgtgc cacttgtcgg gcaacgggcg cagcgccttg gtcaccaggc
     1561 gaatcgagcg cacctcgatc gagagctcgc ccgtcttggt caccatcaac gggccgcgta
     1621 cctgcacgtg atcggcgatg tcgatggcct cgagctccgc gaactcggcc cccatcacgt
     1681 cctgcttggc gaacagctgg atttcaccgc tttcgtcgcg tagccgccaa aagctagcct
     1741 taccgaagcc gcggcgcccc atcacgcggc cgaacagaca aacctcgggc aacgccagcg
     1801 cggcaagccg ttcagcgtcg tagcgcagct cggacttggg cccggccagc gcgggttccg
     1861 cgcgcttgcg cacggcatga acgcgcacgc ggtgcagcgc gtccacgtcg ttcgaaaacg
     1921 ggtctcgccc gcgagctcga taggcctccg ctttctcgcg acgggcctga accagcgcct
     1981 cttcgctgct ttccatgatg tcctcagttg ttcggcaaga tcgtagggtt gccgccgtcg
     2041 gcgttcttga gcaggtaagc ctcgatgaag ggatcgagat cgccgtccag caccgcttgc
     2101 acgtcgctgg tttcccactc cgtccgcaga tccttcacca gctgatacgg ctgcagcacg
     2161 tagctccgga tctgattgcc ccaggcaatt tggcctttgg cggcttcgta gcgcgcctgc
     2221 gccgcttcac gcttcgcctg ctccagctca tagagccgcg ccttcaacat cttcaacgcc
     2281 atagcgcggt tctggtgctg gctgcgctcc gcgcggcaca cgatcatgat gccggtcggc
     2341 aagtgccgga gccgcaccgc ggtctccacc ttgttcacgt tctgaccgcc cttaccgccg
     2401 gcgcgcatgg tcgtgatttc gaggtcggtg tccttcactt cgatgtcgat ctcgtcttcg
     2461 atgtcgggcg tcacttcgac ggccgcgaag ctcgtctgcc gccgcgcatt gccatcgaac
     2521 ggactgattc gcaccaagcg atgcacgccg atctcggcgc gcaggtaccc gaacgcattc
     2581 ggccccgcca ccgtgaacga cgcgccgtcg atcccggctt cgtcgcccac ctggtaatcg
     2641 acgatatcgg tcttgtaacc gtgccgttca caccaacgca ggtattgacg gagcagcatc
     2701 tccgcccagt ccttggcgtc cgtgccgccg gttccgggat ggatgctgac gatggcgttg
     2761 gcgtggtcga cgggaccgga gagcatgcgc cgcagctcag ccgcgcgcac tcgcttggag
     2821 agctccggtg tttggcgctc cagctcggcc acggtgcctt cgtcgttctc ggccgccgcg
     2881 agctcgagca gctcgagcgc gtcattcacg tctcgcgaca gactctcgat cagctcgagc
     2941 ttttcgaccg cggccgcgcg acgacgcata tgcgcctgcg cccggtcctg atcgttccaa
     3001 aagtccggcg cactcgacac actgtcgagc tcgtcgattt ctcgctttaa agcagggacg
     3061 tcaaagagac ccccttagcg catcgacgcg cctttggaga tcctggagca agccccgagt
     3121 ttcggacaac atcgctcggg cttttacagt tttttcagga aatggccagt gaaagccgcc
     3181 ggcgcgccgg tttccatggt ggaaaaccgg atgccggttc agggcccctc cgaggcggcc
     3241 gcgccaccgc gcgccgaccg gcgctcgagc tctgccttga acgccccacc ggtcagcgta
     3301 tccagcgcac tggcgcagaa ggccagctcc gcttcgagca cgagcagacg ctcgggctcg
     3361 aggctggggt ttgccaggcg ggcgcgcgca accagccagc gtgagaagta cttgagcacg
     3421 ctacccacca gacgctcccg cgccgcctcg cgctccgcgc ggagccgctc gatggccagt
     3481 tcttcatccg caaagacgag ccgatcgagc ttccacgtcg cgcgcgcctc gaacacccaa
     3541 cccgcgccac ccgtatgcgt aaagtcccag gcgctgctcg ccgagggaga aagccgcagg
     3601 gactcatccg tcgagcgcaa ggctcgcagc atcaactccg gcaaggccgc cgagctgcgc
     3661 gcgcgcgccg ccattgcctc gaggcgctgg gcaatcgaga catcgccccg cttgcgcaaa
     3721 gccgctgcgg ctaagcgcaa cgccatgccc cagtccaagg gccgcgaggt ctgacgctcc
     3781 ctgggcgacg gcggatccgc gcgctccgtg tcttcagggc gcggctcgtc agtcactttg
     3841 cccgctgaga ccttcgccgc gaccaagagc ggagccaccg agctcgggcg aggtgcagca
     3901 atacgatcca agggcaactc gaccgcgagc atgcccgccc aacgcaaccc actgcccttg
     3961 ctctcgaacg aggctcggca ttctagattg gccaccacgc gcagagagtc agcgcgggcc
     4021 tcggtcaggc accccactgg gcaaagcact ccgagcacga accaagctgt tttcatgcca
     4081 attgctcggc cgtggcgcac gacgagttgc cgaaatagcg cgcccctcga gcgcagaggc
     4141 cccccggtga aaacaaatga ccaagcgtga gcgggcgccg ctctgccttt tcaggaccat
     4201 gccggcgtat gagagacgcg ctgagctagg gagacgccgc tccgcgggaa tagtcgaaga
     4261 gcagcgcccg ctcagcggcg ctcaaaggcc tcgtttggct cagcggcggt cgaaacctca
     4321 cctcgagccc cgccgctccc cagccgattt ccgtcgcgat cgcgagcggc gcggcggcgc
     4381 ttcggcggaa catcgcaacc cgaattcgag tcgctccccc cacgtgatcg agcacccgcg
     4441 agccgcgcgg accgtccaac ccgagcacat cctccgagcg cgacgcgcca tccgcgttcg
     4501 gagtgaaccc cacgcaacga atctcgaggt cagacgtcgc gaccgcctcc aacgtagaaa
     4561 cgtcccaaca cacgacggga tggggagcct gcgtccgcac caccagcaag gacaccggtg
     4621 gctccggttc ttccatttct atgggacgcg cgggcggctc ggtcggcaac gtggcgactt
     4681 catctttgac atcgccgcca tcggaagagg ccaacgccga gctcgagtcg acttctgccg
     4741 gttcccagcc cgcggtcgcg aatgagccct cgagtgactc ccccgggcga tcgagctctg
     4801 cgaagttggg caacctggga ctctgcagcg acgccggacc agattcgatc acgccatctt
     4861 ctgctagttc gtcggggatc ggctccgcaa tgggctcagc cacgggctcc gcgataggtt
     4921 cggcgatggg ctccgctatc ggttcgggtt ccggctccga caggggccct tcttcgggtt
     4981 ccgggggtgg ctcggagggc ggttcgggcc gaggttcctc ggtgggcaaa accaccggct
     5041 gcggaacggg caccaacgcc gactcctccc cggacgtgga ctcatcgatt cccaagggaa
     5101 acggctccgc cttgggtagc actatgggct gagatgctgt gctaaccccg acgtcggtcg
     5161 caaccggaga gcttgaaact cgcggcaggt gctccgactc gaacaccatc gattcgggcg
     5221 gtggaatcgg ctccgcccag gacggcaact ccgacggggg cgcaaattcc agagaatccg
     5281 cgccttcatc ggcgagcatg ccgtcgtgct cctcgcgact ctctcgcgat gcctcggtcg
     5341 aaccttccgg tgtagctacg tccgatcggc cggcgctcgg cgcggctgca gcgctctttt
     5401 cgttggtcgc tacgctctct tcgttggtcg ctacgctctc ttcgttggtc gctacgctct
     5461 cttcgttggt cgctacgctc tcttcgttgg tcgctgcgct ctgttcgttg gtcgctgcgc
     5521 gctccgtact ggtcgctgcg ctctcctcat cagtagcggc gctctctgta ctgggcgcgg
     5581 cgctctgtga gcgggcattg tcaatcgctg cgctctcctc atcggtagcg gcgctctcgg
     5641 cactggtcgc ggcgctctct gtactgggcg cggtgctctc gacggtcgtt gttttggacg
     5701 cgcgagaccg cgcctcgcgg cggtttacga cccgtagacg cccacgcagc gcgagcgctt
     5761 cggcatgatc gggctcggcc gtgagcacct cctccagcat cgccagcgcg cgctctacat
     5821 gaccttgggt cgcgtagatt tcggcgagcg tcacggtggc caccggcgcc ggacctctgg
     5881 cttcgaattc cgcgcgatcg ccccggatcc gtgcggcggc atccgacatg ttcagccctg
     5941 attcgaccac gctggccagt aagtctcgcg ccactcccaa ccaaccgcgg gcgcgacgct
     6001 gggcgagcgg atcgggctcg gtgagccgca cgatttcgtc gctcaactcc acgcgcgtca
     6061 agagctcagg gcgctcgact cccaccaggc gagccctggc aaccaaatcg tctcggctta
     6121 gacgctccaa gctttgcttg tccactcgac cgctattcag gggcgccagc agcccgaggt
     6181 caaggccttc agcacgaaat agcagcttac ccgcagactc ctttgccggc gtcattaccc
     6241 gaggcgcagc tagcgtgccc cgcgttgcag tagacattcg acgaacacgc acaatcgccg
     6301 caggaaacgc tgcagtcact agcgttacac gtcacttggc cctggcaggc tcctgtgccc
     6361 gagcacagca ctttgcagtc gttcgcactg cacgtggttt gcttggcgca gctcccctgc
     6421 ccttggcagg taatccggtc ggaagccgca ttggccgaga cgtcaccggc gcaggctcct
     6481 gcgccggtgc actcgatccc gcacacattc ccgccgcagc tcacgccatt ggcgcacgtc
     6541 cccgggcccg agcagcggac ctgcatctca ctggccgcgc cgtccacccc gccgctgcaa
     6601 gccttttcgc cggtacattc gacgttacat tgttcccctt ggcagcggat ctttccggaa
     6661 cacgacctgg tcccgagaca ctggaccgtg cagttcgcac ccttcggaca cacgacgccg
     6721 ctccggcacg cttcgttgcc gcacgttacg atgcactcgc gatcatctgg acactccacg
     6781 ggcgaggggc aggtgccctc cgcggtgcag tcgaatacgc agcgtccacc tgggcaagct
     6841 ggctcggtgc tcggagccgt tcccgagcct cccgcttggg ccgagctgcc agctcctccc
     6901 aatccatcag cacctccgct gggcgcaccc ccactgttcg agcccgcgct gttcgagtct
     6961 gagtttcccg cggccccaag tccggccgaa gccacaccag ttccagaacc agcgcccacc
     7021 acgccgcctg ttgcgcctgg atgcatcatc gccgacgtac cgtctcgacc gccctcgcca
     7081 acacccgagc tgcttccacc ccgccccagc acgcccgcgt ctcgacccga gatggcagtg
     7141 ccaggagacg actcggtgga gccgcgaatc tccgcccctc caaaaccttg accgagcccc
     7201 tcctcgtccg aacgacgctc gaacaatgac gcaccggacg cactgccaca ggccacgctc
     7261 atcgagatcg ctgtcagccc aagaacccaa ttcctgatcg ctctcatgct cacccattcg
     7321 gagtgaaaag tggccttccc agcgccggcc acggctctga agctcgagat tcccaagagg
     7381 cgtcgagggc ggcactgcac gcaacctcga cgcaacactg cagatcacga cacgacccaa
     7441 acgcggattc cagatccgac ttcgggataa ggacttagcc ctaacgaatc cggctaatga
     7501 gtggcacgaa ctatctatat ctgtgtgtcg ccgaagaccg gtatccgtcc gtcgctttcg
     7561 acagagtcag tcaacccaac cgacgaaccc gcgagtttgc atccgaccaa gccggcagta
     7621 ttgcatgtct tgccagcttt tcgggcgaga attgcccgtt ctctacgcat tttgcgtcac
     7681 acgatctttg aactatcgcg atatcgagca acccgtcaca gttctgttcc cagccaggtt
     7741 tcacgaacga accattactc gagtccactc attgcttcgt caaagccact cactcttggg
     7801 cagcagattg aatgcgtcgt aaacggtaac gaaggacgag ctcgtaagtg agtttacgac
     7861 cgaggcggca ccagtcccac atccacgcca gtcccacatg cacgcccgcg caaacgagtg
     7921 acggtcttgc tcgcgcgaac cagcatgatc gagaaaactc agcttgaacc tgggcaagcg
     7981 agatgcaggg gccgcttcgt ggatcgggcc agtgggtaac gagcgcgacc tccgtcacca
     8041 gaactcgcaa gcgcagccgc tttaacattc cgttacgtcg ccagcaccgc gcaggccctc
     8101 gagtggtttt cgagaccagc gtgagtccgt tccccgcagt ggcccgggct ccaaacgtgc
     8161 gcccctgggc acgcataacg cgcaaagtgc gcatttgcct cgtggcctag ttgcgcctcc
     8221 atcagcagga cgctctctcg agccagtaat cggagcagct cacgggcata cgctttgcta
     8281 aggctttcag gcacatcgcg gtgcgggaac tggataggac acgcaccgat tcgaaatccc
     8341 gcgaaccgct cgaccgcaca accggtccct agtggttcgc gcacgtaacg gagaccctat
     8401 gcatccttct gacgccctga gtacagtcat gaagaccccc gtcaagacag tctctccttt
     8461 catggaggct gctcaggcgc ttcaattcgc ggaagatacg ggtgttcatc acctaccgtt
     8521 ggtcgagcat gggcaagttc gcggcttggt gtgcacctgc gatttggagg aactcgagct
     8581 gcgcgcacct atctcgcgtg cggtgcgtcg gccacccgtg acggtttcga catcggcgag
     8641 catcgccgac gccatggcga ccatggcccg cgaggcggtc ggctcggtgc tggtgctaga
     8701 gggtcagagc atcgtcggaa tcgtaacgcg cgaggatctg aacgggatcg actggggcga
     8761 cgtgcccggg tttcgctgct cgagctgcgg ctccgtcaag cacctcaagt ccgagggctc
     8821 gcgcggcacg ctttgcttgg attgccgctc tcacgctggg ccagaaactc cgttcgacga
     8881 gacgagcgtc ggcgactgag cgaccaatca ctccgccgtc gaggcgccgc cgaaagcacg
     8941 aattgcaatc aaaaccaagc gccgcctgct cgcggatccg gggcgtagtt cgccaacgct
     9001 tcgcgcgccc gctcggagtc gagcgtgatt tgcgcccgca gctcggccag cccctcgaac
     9061 ttccgttcct cgcgcaggcg cgccatcaaa tggacgcgta gctcggcacc atagaggtcc
     9121 gccgcaaaat ccagcacgtg cacttctacg ctgaagccag cgtcgacggt ggggcgcatg
     9181 ccaaggttca tgacgccgcg cccgagcact cgcgcgccat ccgcttccac tcggtccacg
     9241 agacacgcgt agacgccgaa cggcggcaac gcctccagca cgggcgccag gttcgcggtg
     9301 gggaagccca aagtgcgacc gcgttgggcc cccgagatca ccggccccga gagtgcgtga
     9361 ggccggccca aacagcgtgc gacacccgct aggtcaccta gctcgagcgc ggcgcgcgct
     9421 cgagtcgaag aaaacacgcc gagctcatcc cctacgaggg gctccgcgtg tgcatcgaat
     9481 ccaagctgtt cacccagagc ccgcagcgtg cgcagatcgc cgacccggtc acggccgaat
     9541 cggaagtttt cccccacgat gacggtacgg gcgtgcagcc tttgcaccag gatcgactcg
     9601 acgaactcgg acggcgagag gcgcgacaac tcgtgggtga atggctccac cactacgctg
     9661 agattggggt cagcttcgga ggggtctggc gccaagccac gatcggaagc gcagcggagt
     9721 agcagctcca ccttccgatc cggcagggtc agcgccggcc gcgcagttcc cccgagtacc
     9781 ctggaggggt gaggctcgaa cgtcagcacg cgcggcacga gcccgtctcg ctcggcttgt
     9841 gccagcgcag tctgaatcac cgtgcggtgc ccgaggtgca caccgtcgaa gttgccgatc
     9901 acgatcaggg acgcgcgatc ggaacttaca ttccgtgatc cttcgatttt tcgcgggagc
     9961 acggcgacgc tcttactccg gggggggccc cccgtccaat caaaacccgg cgcccaagcc
    10021 gtcgaaagcg agagcatcgg gggctcttct cggctagcgt gggcgccgtt tgacccccga
    10081 ccaagcggtt caagaagccc gcgagcgcaa acttcgacca gtgtacttgg tgctcggaga
    10141 cgaggcgcac ctcgtgcgcg gcgtcgtcga ggccctccgc acggccacgc tcgaaggcgc
    10201 agtgcccggt ctcaatgaag accatatgga tgccgcccag cgcagtgtgg acgacgcgct
    10261 cagcgcggcg cgtacgctgc cgatgctcgc gcgtcgaagg ctcgtgttgg tgcaacggct
    10321 cgagcactgg gagaaccggg cggcggcaga gaacgccgag aaggccgcgg acaaagcgcg
    10381 aggaaaaggt gtcagcgtcg agcctttcga gcgcttgctc gagtatgcca agtccccctc
    10441 gccgagcacc acgctcatcc tgactgggac ggggatcgac aagcggcgga agctctacag
    10501 cgccgcacgc acggacggct ggctcgttca gtgtgatcag ttgggtcgcg cggagctgcc
    10561 cggcttcgtg gagcgcgagg caaaacggcg tagcgcgcgg ctcggtccgc agattgccga
    10621 tctcttggcc gagttgctgg gtccggagct cggcccggtc gttgacgcgg tcgagcggct
    10681 ggcgctttac gctggcgggg aagtcatcac cgaggagatg gtccaagctt gtgtggtgcg
    10741 gctcaggatg gcgaccgttt gggagctgct ggccgccgtc acgcggcggg atctcggagc
    10801 cgctttgaag acgcttggcg atgttttcga gccgaacgag gcggtccggc tcacgggttt
    10861 gctggcttgg tcggcgcggc aactgattcg attcgaagca gcactgagcg cgggagcttc
    10921 gccacaggac gcggcccagc aggcaggggc accgcctttt cgtgcacgag agcttcagca
    10981 acagatcaag tctttgcccc gccccgtgct ggaagggtgg ctacccattt tggcgcgcct
    11041 cgacggagat ctgaaaggcg gcagcaaact gcctccccag gcggtgctcg aacgagcgat
    11101 cttcgagctg tgtgaccgca gaaccgacgc acgcccgggt tgacgctggc agagctcagc
    11161 ttcgacacac acaaagctcg ataggacggg gtcaacgctg ctcggttcga acccaccgag
    11221 cacgaacacc gtgtttcgag ctctcctcat taggtgcttt tatttcgaga ccgggctttt
    11281 tctcgcattc acgctcgaaa ggcgggacca gttcgggacg ctcacgggca aaaagctgaa
    11341 aagaatcggg ttggggcccg gattcagggc cagctcaact gccgtaggtc ttgaccacct
    11401 catagctcgg cctcatcttc tcgaacgggg cacagtcgaa gcctctggac ccagcactac
    11461 cgaagatgac ccatcctgag cgttcgatcc cccgttcggc cttggaaacc ctggcaagcg
    11521 cacctcttcc gaccgtgcac ggcacgtttc aattgcgcgt gtaccgctgg gaagacgtca
    11581 acgcacaccc ggctctcagc cgggagcatt gcgcactcgt catgggtgac gttcgaggca
    11641 agcgaggtgt gggtgtccga gttcattccg agtgcttgac gagcgaggtt ttcggctcgc
    11701 tcaagtgcga ttgtcgcgag cagttcgagc gtgcccaggc ggagatcgct aggcgcggct
    11761 cgggcgtgct cttgtacctg cgacaagaag gccgcggcat cgggctcgcc aacaaggttc
    11821 gcgcctatgc tctgcaggcc ctcggagcag acacgatcga ggcgaacgag ctattgcatc
    11881 ttcccgtcga tgcgcgcacc tacgacgttg cggccgccat tctgcgcgag ctcggggtgg
    11941 agtcgatcga actcctcacg aacaacccgg acaaggttca gtcgctcaca cggctgggca
    12001 tccaggtgga gcggcgtatg cccgtgcaca tcgctgcgaa tcctttttcg gcgtcctacc
    12061 tcgaggtcaa gcgtcgccgg atgctccacg atttgcccgg tgttcccgct gatgcgaacc
    12121 tgacgctccc cacaagcgac ccctcggctg agtgatttgc tcggctgagt gtgactagct
    12181 tctagctgcc gcaaaagaag tggaccggct tgggcttacc gcgccggctc actagaggcg
    12241 gagcagaccc ccgcgccggc ctcacccgta acggccctcg atgtattctg ccgtcttgtg
    12301 attcgcgggg ttcaggaaca aatcctcggt ccggcgcttc tcgatcagct ctccaagcag
    12361 catgaagacg cactcgtcgc tcgcgcggcg cgcttgcgcc atgttgtgcg ttacgatcac
    12421 gatcgtgtac tggccgcgca gggcccacat caactcttcg atggcgcgcg tcccctccac
    12481 gtccaacgcc gagcacggct cgtccatcag aatgatctcg ggtttcagcg gtagcagccg
    12541 cgcgatacag agcttctgct gttgctcgag ctgaagcgat gtggctcgtt cttccagtcg
    12601 atccttcagg tcgtcccaca gccccacctc actcaaggcc ttgtggacgg cctcgtccag
    12661 aatcgccttc ttgaggttgt tcttcgcatg gtggatgcgc atcccgaaga ccacgttctc
    12721 gtagaccgag atgggcagcg gattcggtcg ctggaaaacc attccgacgg acttacgcag
    12781 ttccgtcaag gaaacgtcgg ggtcgtagat gttcttgccg aggatcttga tctcgccggt
    12841 cgtgcgcacg tacccgtagc gctcgttgac gcggttgaag cagcgcagca gcgtagtctt
    12901 tccgcagccc gacggaccaa tcagagacgt aatctgccca cgagcgatat cgattgtaat
    12961 gcctttgagg gcctgaaagt ccccatacca aaggttcaga tctcgggtgg agatcccgct
    13021 gacctctgta gcgccgggcg gcttctgctc agcaggcgct tctgctcccg cgcctgcaat
    13081 acgctcctgc ttcgagccaa gttgggtcat ggcaacacgt tcgagggtcg ggccgagctg
    13141 gatcatccga agatgccgtt cacgtattcg taagtcttgg ggttctttgg gcggcccgag
    13201 aagatgacgt cgttcttatc gacctccatc acctcaccgt tccacaaaaa caacgtgtgg
    13261 ttcgcgagcc gccgagcttg ttggacgagg ttcgtcacca agatgatggt catgctgcgc
    13321 ttgagttcct tcagcacgtc ctcgatccgc atcgtggtga cgggatcgat ggcgatcgaa
    13381 aactcgtcca ggcagaggat ctccggctga tgagagagag cgcgcgcaat cgtcaaacgc
    13441 tgttgctgcc caccggaaag cttggtgccc aagctatcca ggcgatcctt gacctcgtcc
    13501 cataaggcgg cctgctcgag gcaacgttgg accagctcgt ccagcgcggt cttgtctcgg
    13561 atccccgccg ctcgcggcgc gaacgctaca ttgtcgtaaa tgctcagcgg taagccgacc
    13621 ggcagcggcg cgaccatgcc cacgcggcgg cgcagctcgt acacgttgcg catctggttc
    13681 acgtcctgcc cgtcgatctg aatcgtgccg gacgcgcgcg cccctggcac gaactcgatg
    13741 gttcgattca agcagcgcag caacgaagac ttaccggatt gcgcggggcc gatgatggcg
    13801 aagatctcgt gctcctccac gtcgaacgag atgccgtgga tgacccgctt gtcgccgtag
    13861 cgcagctcga gatctttgac tctgattttg ggtgtcgtgg ggttcatgcc cgtaatcctt
    13921 cggggtgacg gcgtgcggcc atgctcggtc cgcgccgcgc gggtcaccac ttcttgcgcg
    13981 tgcgcagccg ggcccgcagc gcgattgcgg ccgcgtttac ggtcaatacc gccccgagca
    14041 agaccaccgc cgtagcgtac ggcaaagcct tcggagcgtc cgtcacctgc gtggtgatgg
    14101 tgaatagatg catggagagc gccatgcatt gatcccagat gctgtacgca aaggggtcgc
    14161 cttcctcgat cttcttgaag aagacggcgc ccgtgaacat aattggcgcg gtttcgcctg
    14221 ccgcgcggga tacttggaga atcacacccg tcaggattcc gctcaccgag ttgggcagga
    14281 cgatgcctcg aatggtctgc cagcgcgtcg cgccgacgtt ccaacacgcc tcgcggaacg
    14341 ttcgcggtac ggctgcgagc gcctccttgg tgctggcgat gattaccggt aaggtcatga
    14401 ccgcgagggt cagtgacgcc gccaagatcg agcgcccaat ccccgcgaac agcacgaagg
    14461 caccgagacc gaacagcgcg tgcacgatac tcggtacgcc cgcgaggttg acgacggcca
    14521 gattgatgat gcgggtcaac cagttgtcct gcgcgtattc gttgaggtaa accgccgcca
    14581 aaaccccaac cggagcggcg atcaacagcg at
//
LOCUS       DASTKU010000984         6017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_193141, whole genome shotgun sequence.
ACCESSION   DASTKU010000984 DASTKU010000000
VERSION     DASTKU010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193141"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFQ61_35010"
     CDS             <1..559
                     /locus_tag="VFQ61_35010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959773.1"
                     /translation="SPYHALALALSSLALWPIWGLARETSFPTARGYDSSGLLRRAKL
                     ELQKRGFKVAVWARIPDGGSEPDELRLLLRLSAAAEGALGIELGVDAHSSPAGYFSEP
                     FVLVRVQQDSRAQSLLSPPEGWQRGRRGDERVGIWRPRLPALFEFVLLAEELGLQLSE
                     SSAKRRVAGATSRLPRWRVASPAQA"
     gene            complement(598..1545)
                     /locus_tag="VFQ61_35015"
     CDS             complement(598..1545)
                     /locus_tag="VFQ61_35015"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET9959774.1"
                     /translation="MATLEFKLKPTHVGARIERLLMELLSCSRSEARALCESGAVRID
                     GRRVKKGERAAAGAVQLQVQAAGVTPEERLVAQPELALEVRLERQDLVVVRKPAGMPS
                     APLALGERGTLANALIARYPEMLAFGYSPREPGLVHRLDTQTSGLLLAARDAATFEQL
                     VQALKRGSITKRYLAVVPRAGLNDRDHIVAPLAPGPKGRVIVAGSGTPYRKDAETRLR
                     VIERGPRFALVEVEAPHAFRHQVRVHLAYAGHPIVGDKQYGGEAHALIGARHALHASH
                     LSLESSGNASVPSFSVDDEVPAEFAALLRSEADSTTAAV"
     gene            complement(1555..2817)
                     /locus_tag="VFQ61_35020"
     CDS             complement(1555..2817)
                     /locus_tag="VFQ61_35020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959775.1"
                     /translation="MVEPSLPTDLREDPELVAVAQKVEAVLHEAVQDLGLSVDISLRP
                     SRSARRSEDTELTRATREHWLIAPRLERRSGALWLRLTVVAPRSRVLHVAYDVLDPED
                     VEMRVVVMLRDVMTAARPGERTREPNLHPNAPEAEPAARTRSEGRGILALNAALFGGF
                     VGFSLQRASGSEDERLIYPLAALGAGVGLGGSMLVAEEWDIGVGDAWFLASGLWWPIV
                     SGSFLAHGYKAPAQDRYAYGLLGATAGVTVASGALSFFRVNDGGALLAHSGGAFGTLL
                     GGLVELAGRGRTDVTPYRGIGYGSGIGVLVAGALATQTTLPPGRVLMTDLGATLGALA
                     GAAIGSPLLLVEESAGTSSTRNRLWLGSVGMGTLIGGAIGFWSSRPSSPGFALASSGA
                     PSSLRPGVGCIAVTERGAAYGATLSGTW"
     gene            complement(3224..4765)
                     /locus_tag="VFQ61_35025"
     CDS             complement(3224..4765)
                     /locus_tag="VFQ61_35025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959776.1"
                     /translation="MKPRDLPVRPLVRPKRAWLAGACVTALALAGTARAEEPRKFGEP
                     NVLSEPAEITQVVDAFDKDDLFDVHVSLGYQISFKKADILRETQAVQNGLSTGGYLAN
                     TLNVAEYKESTSRLNARADVGLYRDVALVLRLPIILSNERELNGLDGSAEVQSVVLQG
                     APGEQLFSLPFKAPKRGGLEYLAIGVDAALMNQHRDPASPTWLVGLEGRFNLGEPLRA
                     CNSSPVAGELKCADPGDVDRNGVSAPDAGEGTFGGGERKSGVSRGVTALEFHTYASRR
                     IKHIEPYAGFRALFEFPNASSAFGKSDLKGSLINHPPLRGTLLVGLNVIPWEIREQFQ
                     RMILDFRFSGTYVSEGRDYSELFDALGSSHARSLRVPNYAEYQRNPNYNPANPNAAPQ
                     SIIDPNSRRAYFTGLTDVQAHGQYTLSAGFTWQAGEYVKFNLGGSYTLVQSHSLTGDQ
                     ACNSDFTNSLDAAGPCRSSSGDGASATGIPNPNYRRTINDPGQRFRVANSSQLDAWLN
                     ATVMF"
     gene            complement(4873..5802)
                     /locus_tag="VFQ61_35030"
     CDS             complement(4873..5802)
                     /locus_tag="VFQ61_35030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959777.1"
                     /translation="MTKVSLANGAVLGLAVVLLELSFTGCARKTSPRPGPPGVPSAGF
                     PASGGTRLESTTPPAIPSAVPMAPSACRVMRVSGEARIGPHGSASERTIQGNDAISGE
                     DWLDLAQDAAVVVRHGNSAREFSLRGPGLFLPCRNGQEQLVVANGVFRSSPGTGARPG
                     GEFWVFTPHQALWYADADLELEVKAELSRAELRSGKVFPEGAAPLRSGAHVWRATLSV
                     SQRVQACEEAAHAARQAAMDLLEPRTPNPTAGANYGERARAHFVARRLARARCGLAHA
                     VLETAGAPDDAPRLRDQLNRADSLWQLPPTFAR"
     gene            complement(5829..>6017)
                     /locus_tag="VFQ61_35035"
     CDS             complement(5829..>6017)
                     /locus_tag="VFQ61_35035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959778.1"
                     /translation="ADRLADTNRELGAAKEELLLEDVTHEIFDDNGDDDTETEDDGDR
                     RTLVLPPPSLRGGPGRSS"
BASE COUNT          994 a         2002 c         1983 g         1038 t
ORIGIN      
        1 gtcgccctat catgcgctcg ccttggccct ttcttcttta gcgctgtggc cgatttgggg
       61 cctcgcgcgc gaaacctcat ttccaactgc gcggggttac gacagcagtg ggcttctgcg
      121 ccgcgccaag ctcgagctcc agaagcgcgg cttcaaggtc gcggtgtggg cgcgcatccc
      181 cgatggaggc tctgagccgg atgagctccg cttgctgctg cggctttcgg ccgcggccga
      241 gggcgcgctc ggcatcgaac tcggggtgga tgcccactcg agccccgctg gttacttcag
      301 cgagccgttc gtgttggtcc gagtgcaaca ggactcgcgt gcccagagct tgctctcgcc
      361 tcccgagggc tggcaacggg gacgacgcgg cgatgagcgc gtggggatct ggcggccgcg
      421 gctgcccgcc ttgttcgagt tcgttctgct cgcggaggag ttgggtttgc agctctcgga
      481 aagcagcgcg aagcgccgcg tggcgggcgc gacgtcacgc cttcctcgct ggcgagtggc
      541 gtctccagct caggcttgag cccaggctcg agtccgagca ttgaacctag tccaggccta
      601 tacggcggcg gtggtggagt ccgcttcgct gcgcagcagc gcggcgaatt ccgcgggcac
      661 ttcgtcgtcc acggagaagc tcggcacgct ggcattgcca gacgattcga ggctcaggtg
      721 gctcgcgtgc aaggcgtgtc gcgcgccgat gagggcgtgc gcctctccac catattgctt
      781 gtctcccacg atcgggtgcc ctgcgtacgc caagtgcact cggacttgat gacgaaaggc
      841 gtgcggggct tcgacctcga cgagcgcaaa acgcggtccc cgctcgatca cccgcaggcg
      901 cgtctccgcg tcctttcggt acggggttcc cgaaccagcc acgatcacgc gccctttcgg
      961 acccggcgcg agcggcgcga cgatgtggtc gcgatcattc aagcccgctc gagggaccac
     1021 cgcgaggtag cgcttcgtga tcgaccctcg tttgagcgcc tgcactagct gctcgaaagt
     1081 ggctgcgtct cgcgccgcga gcagcaaacc cgaagtttga gtgtccaagc ggtgcaccag
     1141 ccccggctcg cgcgggctgt acccgaaggc gagcatctct gggtaacgag cgatcagtgc
     1201 gttggcgagc gttccgcgtt caccgagcgc taacggagcg ctcggcatgc ccgcaggttt
     1261 tcgcaccacc acgagatcct gacgttccag tcgcacctcg agcgcgagct cgggttgagc
     1321 taccaggcgc tcttccggcg tgacgccggc ggcttgcact tgcagttgga ccgcgcccgc
     1381 tgcggcgcgt tcgcccttct tcacccggcg gccgtcgatc cgcacggccc cgctctcaca
     1441 tagcgcgcga gcctcgctgc gcgagcatga gagcagttcc atgagcaggc gctcgatgcg
     1501 cgcgccaaca tgtgtgggct tcagcttgaa ctcgagggtt gccacgcgcg agcgttacca
     1561 cgttcccgac agcgtggcgc cgtaggccgc acctcgctcg gttacggcga tgcaaccgac
     1621 cccgggtcgc aggctgctgg gtgcgccgct gctcgccaga gcgaaaccgg gagagctcgg
     1681 gcggctgctc caaaagccaa tggctccgcc aatcaatgtg cccatgccga cactgccgag
     1741 ccagagtcgg ttccgcgtgg atgaggtccc ggcgctctct tcgaccagca acaacgggct
     1801 gccaatcgcc gctccggcga gcgctcccag ggtcgcaccc aagtcggtca tcaagacccg
     1861 gcccggggga agcgtggttt gcgtggccag cgcgcctgcc accaacactc cgatgccgga
     1921 gccgtagccg attccgcggt acggagtgac atcagtgcgg ccacgccccg cgagctccac
     1981 gaggccgccg agcagcgtgc cgaacgcgcc gccggagtgt gccagcagtg cacccccgtc
     2041 attcacacga aagaagctca gcgcgccgct cgcaacggtg acgcccgcag tggctccgag
     2101 caggccatag gcgtatcggt cttgagcggg ggccttatag ccatgggcga ggaacgagcc
     2161 cgagacgatc ggccaccaca agccggaggc gagaaaccaa gcgtcgccga cgccgatgtc
     2221 ccattcctct gcgactagca tcgagccacc caagcccacg cccgcgccga gggccgccag
     2281 cgggtaaatc aagcgttcgt cctccgagcc gctcgcgcgc tgcaacgaga agccgacgaa
     2341 gccgccgaac aaagccgcgt tcagcgcgag aatgccgcga ccttccgagc gcgtgcgcgc
     2401 ggcgggctcg gcttcgggcg cgttggggtg caggttgggc tcgcgggtgc gttcgccggg
     2461 gcgtgctgcg gtcatcacgt cgcgcagcat caccacgacg cgcatctcga cgtcttccgg
     2521 atcgagcacg tcgtaagcca cgtgtaagac gcgcgaacgg ggcgctacca ccgtcaatcg
     2581 cagccacaac gctcctgagc gtcgctcgag cctcggcgca atcagccaat gctcgcgcgt
     2641 ggcgcgcgtg agctcggtgt cttccgagcg acgtgcggag cgcgaggggc gcaacgaaat
     2701 atccacggag aggcccaaat cctgcactgc ttcgtgcagc acggcctcca ccttttgcgc
     2761 caccgccacg agctcggggt cctcgcgcag atcggttgga aggctgggtt ccaccacgcg
     2821 cggccttaaa atctctactt ccctggatcc accggcgtgg gttgcttctg acggcgcgta
     2881 ggagttactc gggctctcct cattggctcg aggccctcga ggaaggaccg gggctgccga
     2941 gtcctcgccg atctgtgggc catttctgtc tggcgcggcg atgggagagg gaggtgctgg
     3001 gctctgctga ggtgctgggc tctgctgagg tgctgggctc tgttgaggtg ctgggctctg
     3061 ctgaggtgct gggctctgct gagccgctgc aagcggagtg agcgcggcgc tgaaggcgcc
     3121 gacgaggctg gccatggctc gcgagcgcca cccagcgaaa ggcctttgct cgaagagggc
     3181 ggcccggatc cggcgcttgc ccgtgccgag gatgcggcac cgctcagaac atgacggtcg
     3241 cattcaacca agcatccagt tgtgaggaat tggccacccg aaagcgctgc ccaggatcat
     3301 tgatcgtgcg acgatagttc gggttcggga tcccggtcgc gctagcgccg tcgccggaag
     3361 agctgcggca aggccccgcc gcgtccaggc tattcgtgaa gtcagagttg caggcttgat
     3421 cgcccgtcaa cgagtggctt tgcacgagcg tgtacgaacc ccctaagttg aatttcacgt
     3481 actcgcccgc ttgccaggtg aagccggcgc tgagcgtgta ttggccgtgc gcttgcacgt
     3541 ccgtgagacc ggtaaagtac gcgcgtcgtg agttcggatc gatgatcgac tgaggcgccg
     3601 cgttcggatt tgcgggattg tagttggggt tgcgttggta ttcggcgtag ttcggtaccc
     3661 gcaacgagcg cgcgtgactc gaccccaagg catcgaacag ctcggagtag tcccgtcctt
     3721 ccgagacgta ggtcccggag aaacgaaaat ccaagatcat gcgctggaac tgctcacgga
     3781 tctcccaagg gatcacgttc aagcccacca gcagcgtgcc gcgcagcggt ggatggttga
     3841 tgagggagcc tttcaagtcg ctcttgccga acgcgctgct ggcattcggg aactcgaaca
     3901 aggcccgaaa ccctgcgtag ggctcgatgt gtttgatgcg acgcgaggcg taagtgtgaa
     3961 actcgagcgc cgtgacaccg cggctcacac cgctcttgcg ctcacccccg ccgaaggttc
     4021 cttcaccggc atcgggggcg ctgacgccgt ttcggtcgac gtcaccgggg tccgcgcact
     4081 tcaactcgcc cgcgacgggg ctgctattgc acgccctcag gggttcgccc aaattgaacc
     4141 ggccttccaa gccgacgagc caagttggac ttgccggatc gcggtgctgg ttcatgagcg
     4201 ccgcgtccac tccgatggcg agatattcca gcccgccccg cttgggcgcc ttgaacggca
     4261 agctgaagag ctgctcgccc ggtgcgcctt gcaacactac gctctgcact tcggcgctac
     4321 catccaaccc gttcaactcg cgttcgttcg agaggataat cggcagcctc aacacgagcg
     4381 cgacatcgcg gtacaagccg acatccgcgc gcgcgttcag gcggctggtg ctctccttgt
     4441 actcggccac gttgagcgtg ttggcgaggt agcctcccgt gctcagtccg ttctgcacgg
     4501 cctgcgtctc gcgcagaata tctgccttct tgaagctgat ttggtaaccg agcgagacat
     4561 gcacatcgaa caggtcgtcc ttgtcgaagg cgtccacgac ctgggtgatt tcggcgggct
     4621 cgctgagcac attgggctct ccgaacttgc gaggttcttc ggcgcgtgcc gtgcccgcga
     4681 gggccaaggc cgtcacgcac gcgccagcaa gccaggcgcg cttggggcga acgaggggcc
     4741 tcacgggcaa atcgcggggt ttcattcgaa gatgcccgtc ggacgctagc ggtcgcagcc
     4801 agaaactgtc aacggaacac acccgcccca tccaaatccg ccggcctcca cgctcgagcg
     4861 ctccttaatc ggtcaacgcg cgaaggttgg aggcagctgc caaaggctgt cagcgcggtt
     4921 caactggtcg cggagccggg gcgcgtcgtc gggtgcaccg gcggtttcta gcaccgcgtg
     4981 cgccaatccg cagcgggccc gcgccaggcg tcgcgcgacg aaatgagcgc gagcgcgctc
     5041 gccgtagttg gcgccagcgg tcgggttcgg ggttcgaggt tcgagcaggt ccatggccgc
     5101 ttgccgcgca gcgtgagccg cctcctcgca ggcctgaact cgttgactga ccgagagcgt
     5161 ggcgcgccac acgtgagcgc ccgaacgcaa gggtgctgca ccctcgggaa agactttgcc
     5221 ggaccgcagc tccgcccgcg acagctccgc cttcacttcc agctcgagat ccgcgtcggc
     5281 ataccaaagc gcctgatgcg gcgtgaacac ccagaactcg ccgcccgggc gagctcccgt
     5341 tcccggagaa ctccgaaata caccgttggc cacgacgagc tgctcttggc catttcggca
     5401 cggcaaaaat aggcccggac cgcgcagact gaactcgcgc gcggagttgc cgtgtcgcac
     5461 cacgaccgcc gcatcctgcg ccaaatcgag ccagtcttcc ccggaaatgg cgtcatttcc
     5521 ctggatcgtg cgttcgctcg cggacccatg aggcccgatc cgagcttcgc cggagacgcg
     5581 catgacgcgg caggcgctcg gtgccatcgg gaccgcgctt ggaatggcgg ggggcgtcgt
     5641 cgactccagc cgggtaccgc cggaggcagg gaagccggcg ctagggacgc ccggtggacc
     5701 tgggcgaggt gatgtcttcc gcgcgcatcc ggtaaagctc agctcgagca gaaccacggc
     5761 gaggccgagg accgcgccgt tggccaacga gactttcgtc aacgagacgc cctcggactc
     5821 cgattcgact agctactacg cccggggccg ccccggagcg aggggggagg taacacgagc
     5881 gtccgccgat caccgtcatc ctccgtctcc gtatcgtcgt cgccgttgtc gtcgaagatc
     5941 tcgtgcgtca cgtcttcgag cagcagctct tccttggccg ccccgagctc tcggttcgta
     6001 tccgccaagc gatcggc
//
LOCUS       DASTKU010000985         9973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_414699, whole genome shotgun sequence.
ACCESSION   DASTKU010000985 DASTKU010000000
VERSION     DASTKU010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9973
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414699"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..568
                     /locus_tag="VFQ61_35040"
     CDS             203..568
                     /locus_tag="VFQ61_35040"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET9959779.1"
                     /translation="MPRAVPIELRTGSGDVRMIEMRGNVNVETGSGDIDGTALEGADV
                     SAHTGSGDVTLETLAAETIYVQTGSGDVKLWVPSGEYQLDVTSGSGDRTIRGVTDAAR
                     ATSEIYVHTGSGDLVIQGR"
     gene            658..1200
                     /locus_tag="VFQ61_35045"
     CDS             658..1200
                     /locus_tag="VFQ61_35045"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET9959780.1"
                     /translation="MAAAKKTNSKKKTNSKKKTNSEPVKAKARETLNLAEKPKASKKA
                     KASAAPKPSQGRSRKPQPRADYGAPVDDFFAKQSGPMRGLLDQLRELIEAAVPGAESA
                     LKWGMPFYTRGGKTVCALGAHKAHVNLILSGPPGTFADPKGLLEGDGKTGRRLKLTPS
                     STIPVTEVRRWLKAAAALAD"
     gene            complement(1217..1975)
                     /locus_tag="VFQ61_35050"
     CDS             complement(1217..1975)
                     /locus_tag="VFQ61_35050"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9959781.1"
                     /translation="MASFDAPVQDFMRRELIAVGPNATLGTISALLERHDISAVPVLE
                     DQKLRGIVSIADILRVESDRTLSLTAADVMRTPVVTVAPESPLREAARRMSEHRIHRV
                     IVCEGQRPVGVLSTRDAMLALEAPPVMAPVREVATASVLTVDLSDSIDSALALLEASN
                     TRGLVVLDEDWPVGVFTQTEALRARALPERQRADPVEHVMSYETICMNAATPLYRVAR
                     QARAVRVRRILVVDHRQLCGIATGFDLVRWIGEH"
     gene            2383..3372
                     /locus_tag="VFQ61_35055"
     CDS             2383..3372
                     /locus_tag="VFQ61_35055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9959782.1"
                     /translation="MPKQLSIVIPTYNRRERLGRVLRALSEQTVDLSSFEAVVVDDGS
                     KDGTSEWLKEQSFGFEVQVLSQTNRGPAEARNAGIDAAQGELVLFLDDDVVPEPQLIE
                     EHLKSHAAEADVVVMGPLVSLDHYEQPWVAWEQEKLERQYQAMTRKEWEPTYRQFWTG
                     NASIARAHLLAAGKFDPTFKRGEDVELGLRLHERGLKFRFNGRARGLHHAERSLDSWE
                     HAHRSYGRLEAEMLSSRPNDEVVSILSGNWRRLNPLTRAAARLCIQSPALNQGATRLL
                     REALKTEPMRRSSFVSSKACSLLANILYWRESAAVLGPDRLKRVFRQARKSTS"
     gene            3537..4175
                     /locus_tag="VFQ61_35060"
     CDS             3537..4175
                     /locus_tag="VFQ61_35060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959783.1"
                     /translation="MALGVIVGIRLVSCWTIGAWLGAGLSLQLVGLGWVCAQNTVLLP
                     PLRAADASSVPSESNVSEDDFPPPPAPPRLMRLFLGDETRRAPGRRFSISLDAPAFAN
                     LAVEGAKPLGRKIRTSLDLGEEPAGESLAPAPGEDVAKANSSERELVRIGASSVARFP
                     ELDSDPSVMSLGHVQVRSSLTLESMPAPRAVRRDLDVETAVDYRRWFRTSLE"
     gene            4358..4741
                     /locus_tag="VFQ61_35065"
     CDS             4358..4741
                     /locus_tag="VFQ61_35065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9959784.1"
                     /translation="MIVDDAALMRTVIRNVLLDDPGFQVVASVENGAKALEELKNQPI
                     DIVICDIEMPVMDGLTFIRHARLKTRAKIVVLSSVAGMGSQQATEARRLGADAVIQKP
                     SGSVSLDLGQKMGKQVLDILHKVAG"
     gene            4759..5256
                     /locus_tag="VFQ61_35070"
     CDS             4759..5256
                     /locus_tag="VFQ61_35070"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HET9959785.1"
                     /translation="MSYDEVDSLDEESADNTFLTFHVGGEEYAVPVAQVTEIVRLQKT
                     FAVPDVPAYIRGVMNLRGKVIPLLDVRSRFGLAEEVYTDRTVVVVLEVGDAPTGLVVD
                     GVSDVTEISPDQIEPCGVTHRRGKQAMVTGMGKRESKVSFILDLTALLNIEPPQVSEA
                     GTLTH"
     gene            5272..7284
                     /locus_tag="VFQ61_35075"
     CDS             5272..7284
                     /locus_tag="VFQ61_35075"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HET9959786.1"
                     /translation="MTTIPKKQTARASKSTASKGASVSKAQLSKPQISQEELQDMQGQ
                     ISAISRTMAVIEFDLDGTIRSANDNFLAAVGYRREEVVGRHHRMFVEPAYADSAEYRE
                     FWEKLRAGQFHAGEFKRVGKGGRLVKLQASYNPIFDAQGRPYKVIKYAADRTADDNEA
                     RAKEMVEMLRIRSALDGSTTAVMIADTNFNIVYMNAAVKKLLTTHEAAIQKDIPQFQV
                     EGLLGRSIDSFHRNPAHQRNVLQKARGSHFARISAGGRRMDLMTTTAIGADGKVAGYT
                     VIWNDVTDQVNAQNDVERVLKAAIDGDLTQRLEASQYEGFLKSIAEGMNHLLDSVAES
                     FGHVKVAIDQVGEASTQLRSTSQLMSSSSVGLNTAAMDSAGALQRSSEMVNANAENAA
                     MANQLVSLTSTAAQSGTSRMQEMSNAMSEINSSAQQIAKIIKVIDEIAFQTNLLALNA
                     AVEAARAGRHGKGFAVVAQEVRSLAERSAKAAKETAQLIEDSVSKVADGVRIADATRG
                     ALQEIVGNVGKVVDLVGEIATASSEQSRTIKQVTDSMGQVTEGAQAGSQQSNEVAAAA
                     EEMSRQMEIVRKRMAKYRVPSVNNGGLPDVSPEMLAQLANLLRQQGVSLPGNGFRPPP
                     LPAPIHLSGSNGHSHSNGHANGHKNGHSDPKSVLPLDRDERGFGGF"
     gene            7467..>9973
                     /locus_tag="VFQ61_35080"
     CDS             7467..>9973
                     /locus_tag="VFQ61_35080"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HET9959787.1"
                     /translation="MGLEDNELWQEYSNDGSEALKDIEESLLVLETNPKDKSQINRLY
                     RGLHTLKGNSGFLRLVQIEHLAHVSEDLIGLVRDHGVVMDEAIVELMLRVVDRLNQVV
                     TQAGDQHRDADPSQVHDLTEAVSELMRQRQSGALEATPAASATNMSVANGRAAKPSEA
                     PPFADIGPVVESEAPSGDNMDWIWGANSVAPTAKIDPAVDAGYLEVFLSVAEPALNAI
                     SAAIAATISSDEVDEAREKLNGQCAELGLAAERMGYVHIVDLLETIRRAAIEGGTALT
                     NLSALEMALYSQLVLIESNYRSLVSEAQDFGIVRSYQRACANVALADLARLRYLLDEP
                     EQLSELEVRDLFQRLRAACEYYGFTGAARACLEREDELCRVLEQSAQLDNEVFAHASA
                     LITELGQTIYAVNAGEDLSDIPVQEAADDPMKFDPPVLALPISEEIRRQLTRVGLSAI
                     GDFKAQGVAVLEVRAELEKHPGVIDRFNEWLGSGEVSLVSSAVLDSEIPGNPPAYLFL
                     VGASAPKARSALGVLDPSGNIFQLRRLDQQEAEPPRRKPAPVEAAFSPVQEPVILLAP
                     EAPPARAATPQQATPQAGPSRAAAALVRGVSASSNAQSPEPSPGEGEPEARESSEARD
                     PSEAKEPSEAKESSEAREPSNKAEFLRIDARKVSLIMDLAGEIGLACGAVTHHPDIES
                     MDLEGFSAASHKLEMLIRELQNEVSGMRLVPVAGVFQRMKRVVRDAARRTGKKVELVL
                     VGEETEIDKVMVDSLAEPLVHMLRNAVDHGIESPDERSAAGKPAVGRIVLEASHQGGE
                     VTVEVSDDGRGLDLNRILSRARERALIPADANPTEEE"
BASE COUNT         1961 a         2993 c         3324 g         1695 t
ORIGIN      
        1 ggagctcggc agtgtccgcg ctctgacggt cgatgtggga agtggagacg ttacgattcg
       61 tggtgcgctc gtataggggg tacaagtcac ggcgcgaata gagggcgata ccaatcacct
      121 cgcgcacgcg ctcgatggtg aacggctgat gctcttcgac gactgtcagg agagccactg
      181 ttcggtagac atccaggcgc gagtgccgcg ggcggtacca atcgagctgc ggacgggctc
      241 cggggacgta cgtatgatcg agatgcgcgg caacgtgaac gtcgagaccg gctccggaga
      301 tatcgatggt accgccttgg aaggcgcaga tgtctccgcg cacaccggct ccggcgacgt
      361 aacgctcgaa actctagccg ccgagaccat ctatgttcaa accggttccg gcgacgtgaa
      421 gctgtgggtg ccctctgggg agtaccagct ggacgtgacg tcgggctctg gagaccggac
      481 cattcgaggc gtgaccgatg ctgctcgggc gacatccgaa atatacgtac acaccggctc
      541 tggagacttg gtaatccagg gccgttgagt gacagcggtt gagtaaaaga acgcaatcga
      601 tccccactcg agcgttgagc actttcctcc tggcagcggt atgccactat cgcggccgtg
      661 gcagccgcaa agaaaaccaa ctcgaaaaag aagaccaact cgaagaagaa gaccaactca
      721 gagcccgtca aagccaaggc tcgagagacg ctgaacctgg cagagaaacc gaaggcgtcg
      781 aagaaagcca aagcctcggc cgcgccaaag ccctcgcaag gccgcagccg caagccgcaa
      841 ccgcgggccg actacggcgc gcccgtagac gacttcttcg ccaagcaatc ggggcccatg
      901 cgggggcttc tcgaccagct gcgcgagctg atcgaggccg cggttccggg cgccgagtct
      961 gcgctcaaat ggggcatgcc cttctacacc cgaggcggta aaaccgtatg cgcgttaggg
     1021 gcccacaaag cacatgtgaa tctgatcttg tcagggccgc ctggcacctt cgcggatccc
     1081 aagggcttgc tggaaggtga cggaaagacg gggcggcgcc tgaagctcac gcccagctcg
     1141 acgattccgg tcacggaagt gcgtcggtgg ctgaaagccg cagctgcgct cgcggattga
     1201 acacgaattc ttcagctcaa tgctcgccaa tccaacgcac gagatcgaag ccagtagcga
     1261 tgccgcacag ctggcgatgg tccacgacca ggattctacg cactcgaacc gcgcgcgcct
     1321 ggcgcgccac ccggtataga ggcgtggccg cgttcataca gatcgtctcg tagctcatca
     1381 cgtgctctac cggatcggcg cgctggcgct ccggcagagc acgtgctctg agcgcctcgg
     1441 tttgagtgaa cactccaacc ggccagtctt cgtcgagcac cacgagccca cgggtattcg
     1501 acgcctccaa taacgcgagc gcggaatcta tcgaatcact cagatctacg gtgagtaccg
     1561 aagccgtggc cacctcacgc accggcgcca tcaccggcgg cgcttccaac gccagcatcg
     1621 cgtcccgcgt ggagagcacg ccgaccgggc gctgcccttc acacacgatg acgcgatgaa
     1681 ttcggtgctc ggacatgcgt cgagccgctt cccggagcgg gctctcggga gccacggtga
     1741 ctacgggcgt gcgcatcacg tctgccgccg tcagagatag agtgcggtcg ctctccaccc
     1801 gcagaatgtc cgcgatcgag acaatcccgc ggagcttctg gtcttcgagc accggcacgg
     1861 ccgagatgtc gtggcgttcg agcagcgcac tgatggtccc gagggtggcg ttggggccga
     1921 cggcgatcag ttcgcgccgc atgaaatcct ggactggagc atcgaacgag gccatgttcc
     1981 gaggttcttt actacgttgc ttcgcgagcc gaccaaggat ctgtcatgga tcatggccgc
     2041 cgcgagacaa cgagcgcgcc ggtcgtccga atcggtcttt tggccaccgg tttacggctc
     2101 ctcatcccca acgagccgcg ccggcgcccc aatcgagaaa catagcgggc ccggtctgcg
     2161 gaggtggttc gcgggggccg gcgcggtttt gcgcgctaga gtggcgcgaa tggactgcgc
     2221 tggcgggcgg ggcgccgtgt gtcgcgcacg tttacacggc ggcccaaacc agaatggctg
     2281 tgctcgtcgg gtcctagggt tcggggatga atttggtcaa aacgtgcgga tagccgcgga
     2341 gcgctggacc ttccgcgagg cgacgtctag aaaaagcacg tcgtgccgaa acagctcagt
     2401 atcgtgatcc caacatacaa ccggcgtgaa cgcttgggcc gggtgctccg ggccttgagt
     2461 gaacagaccg tggatctttc gagcttcgag gcggtcgtcg tggacgacgg gtcgaaggac
     2521 ggaacttcgg agtggctgaa ggagcagagc ttcggtttcg aagtacaggt gctttcgcag
     2581 acgaatcgcg gcccggccga ggctcgcaac gccggcatcg acgccgcgca aggggagctg
     2641 gtgctgttct tggacgacga cgtcgtgcca gaaccgcagc tgatcgagga gcatttgaag
     2701 tcccacgcgg ccgaagcgga cgtggtggtg atggggccac tcgtttctct agaccactat
     2761 gagcaaccct gggtggcatg ggagcaggag aagctcgaac gccaatacca ggcgatgacg
     2821 cgcaaggagt gggagccgac gtatcggcag ttttggacgg gcaatgcctc gatcgcccgg
     2881 gcgcacctgc tggcagccgg taagttcgat ccgaccttca aacggggcga agacgtggag
     2941 ttgggtttgc ggctgcacga gcggggcctc aagttccgct tcaatggccg agcgcgtggt
     3001 ttgcaccacg cggagcgttc gctcgattcc tgggaacatg cccatcgcag ctatgggcgg
     3061 ctcgaagcgg agatgctgtc gtcgcggccg aacgacgagg tagtctcgat cttgagcggc
     3121 aattggcgcc gtttgaatcc gctgacgcgc gcggccgctc ggttgtgtat tcagagcccg
     3181 gccttgaacc agggtgcaac tcggctactc cgcgaagcgt tgaagacgga gccgatgcgc
     3241 cgcagtagct tcgtctcgag caaagcctgc agcttactcg ccaatatctt gtactggcgt
     3301 gaatcggccg cagtcctggg ccccgaccgg ttgaagcggg tgttccggca agctcggaaa
     3361 tccacgagct agtgccgcgc atgtgagaag acacaggaaa tttcgggcgc tgcgctagtg
     3421 gctcgcgccg gctagctctc gggcgtcagg gtctcgctcg ccacgcgccg agacgagggg
     3481 cttcggcgct tgcccgcccg gagccagggg cgcatactga cgccagtccg gttcgggtgg
     3541 cgttgggtgt catcgtgggt attcgcttgg tgagctgttg gacgatagga gcatggttgg
     3601 gcgcagggct gagcttacag ctcgtcggcc ttggctgggt ctgcgcccag aatacggtgt
     3661 tgctgccgcc gttgcgtgcc gcggacgcga gcagtgtccc aagtgaatcg aacgtctcag
     3721 aggacgactt tccaccgccg ccagcgccgc cgcgcttgat gcgcttgttc ttgggagacg
     3781 agactcggcg cgcgccgggg cgtcgattca gcatctcgct agacgcgccg gcgttcgcga
     3841 atctcgcggt ggagggtgcc aagccccttg gacgcaagat ccggacatcg ctcgacctcg
     3901 gggaggaacc cgctggcgaa tccttagctc ctgctccagg agaggacgtc gccaaggcca
     3961 actcctcgga gcgagaactc gtgaggattg gcgcgagctc cgtggcgcgc tttccggagc
     4021 tggactcgga tccaagcgtg atgtccttgg ggcatgttca agtacgctct tccctgactc
     4081 tggagtccat gcccgcgccg cgcgcggttc gacgggattt ggacgtcgaa acagccgtcg
     4141 attaccgccg ctggtttcgg acatcgctag agtaaggtcg cgccggagcc ctcgaggtcg
     4201 gcgcgtcagg acctcccgag cgagaccaac tgccgcgtag caagttgccg cattcggatc
     4261 taaaggccgc gttgggttcg accgttggac ccaacacctc atgttgcgtt ctccaccagg
     4321 gcagcccgcc aaaacaactg catctgcacg acgagtcatg atcgtcgacg acgcggcgct
     4381 gatgcgcacc gtcatccgaa atgtgctcct cgatgacccg gggtttcagg tcgtcgcgag
     4441 cgtcgagaat ggcgccaagg cgctggaaga gctgaaaaat cagcccatcg atatcgtgat
     4501 ctgcgacatc gaaatgcccg tgatggacgg actcacgttc atccgacatg cacgcctgaa
     4561 gacgcgcgcg aagatcgtgg tgctgtcatc ggtggcgggc atgggctcgc agcaggcgac
     4621 ggaggcgcgg cgtctcggcg cggacgcggt gattcagaag ccgtcgggat cggtgtcgct
     4681 cgatctcggg cagaagatgg gcaagcaggt gctcgacatc ctgcacaagg tggccggttg
     4741 agtcagcgag gagtcgggat gtcatacgac gaagtggata gcttggatga agagtctgca
     4801 gacaatacgt tcctgacctt ccacgtaggg ggagaggagt acgcggtgcc cgtcgcgcaa
     4861 gtcacggaga tcgtgcgctt gcaaaagacc ttcgcggtgc cggatgtgcc ggcttacatc
     4921 cgcggcgtga tgaacctacg aggcaaggtg atcccgctgc tcgacgtgcg cagtcgcttt
     4981 gggctggccg aggaggttta cacggatcgc accgtcgtcg tggtgctcga agtcggggat
     5041 gcgcccaccg ggttggtggt cgacggcgtc tccgacgtaa ccgagatttc tccagatcaa
     5101 atcgaaccct gcggagtgac gcatcggcgt ggcaagcaag ccatggtcac cggcatgggt
     5161 aaacgtgagt ccaaggtgag tttcatcctg gatttgaccg cactcttgaa catcgaaccc
     5221 cctcaggttt ccgaggccgg gactctgacg cactgaacga gaaggaaagg catgacgacg
     5281 atacccaaga agcaaaccgc ccgagcctcg aaatcgactg cctccaaggg cgcttcggtt
     5341 tcgaaggccc agctctccaa gccccagatc tcgcaggaag agctgcaaga catgcagggg
     5401 caaatatcgg caatttcccg caccatggcc gtgatcgaat tcgatctgga cggcacgatc
     5461 cgaagcgcga acgataactt tttggcggcg gtgggctacc ggcgcgaaga ggtggtgggc
     5521 cgccatcacc gcatgttcgt cgagccggct tacgccgatt cggcggagta tcgggagttt
     5581 tgggagaagc tgcgcgcggg gcagttccat gccggtgagt tcaagcgagt cggcaagggc
     5641 ggaaggctcg tcaaactgca ggcctcttac aatcccatct tcgacgctca aggccggcct
     5701 tacaaggtca tcaagtatgc ggcggaccgc accgcggacg acaacgaggc gcgcgccaaa
     5761 gagatggtcg agatgttgcg cattcgcagc gcgctcgacg gctcgaccac ggccgtgatg
     5821 atcgcggata ccaacttcaa catcgtatac atgaacgctg cggtgaagaa gctgctgacc
     5881 acccacgaag cggccatcca gaaggacatt ccgcagttcc aggtggaggg gctgctcggg
     5941 cgctcgatcg actctttcca tcgcaaccca gcccaccaac gcaacgtgct ccaaaaggct
     6001 cggggcagcc acttcgcgcg catctcggca ggcggacgcc ggatggactt gatgaccacg
     6061 accgcgatcg gcgccgacgg caaggtggct ggctatacgg tgatctggaa cgacgtcacc
     6121 gaccaggtca acgcccagaa tgacgtcgag cgggtgctga aagccgccat cgacggcgac
     6181 ctcacgcaac gcctggaagc ctcgcagtac gagggcttct tgaagtcgat cgcggaaggc
     6241 atgaatcacc tgctcgactc ggttgccgag tcgttcgggc acgtcaaggt ggcgatcgat
     6301 caggtgggtg aggcgtcgac ccagctgcgc tccacgagcc agctgatgtc ctcgagctcg
     6361 gtgggtctga acaccgccgc gatggactcg gcgggcgcgc tgcagcgctc ttcggagatg
     6421 gtcaacgcca acgcggagaa cgcggcgatg gccaaccagc tcgtctctct cacttcgacg
     6481 gccgcgcaga gtggaacgtc gcgcatgcag gagatgagca acgcgatgag cgagatcaac
     6541 tcctccgccc agcagatcgc caagatcatc aaggtgatcg acgagatcgc gttccaaacc
     6601 aacctgctcg cgctgaacgc ggcggtggaa gccgcgcgcg cgggacgcca cggcaagggc
     6661 ttcgcagtcg tggcccaaga ggtgcggagc ttggctgaac gtagcgccaa ggccgccaag
     6721 gagacggcgc agctgatcga ggactccgtt tcgaaggtcg ccgacggcgt gcgcattgcg
     6781 gacgccactc ggggtgcgct tcaggagatc gtgggcaacg tcgggaaggt cgtggatctg
     6841 gtcggagaaa tcgcgaccgc cagcagcgag caatcccgca ccatcaagca agtgacggac
     6901 tcgatgggtc aagtcacgga aggtgcgcaa gccggcagcc agcagagcaa tgaagtggcc
     6961 gccgccgcgg aagagatgag ccgccagatg gagatcgtgc gcaagcgcat ggcgaagtat
     7021 cgcgtgccga gcgtcaataa cggcggtttg ccggacgtgt cgcccgagat gctcgcacag
     7081 ctcgcgaacc tgctccgcca gcaaggcgta tccctgcccg gtaacggctt ccgcccgccg
     7141 cccttgcccg cgccgatcca cctatcgggc agcaatgggc actcgcacag caatgggcat
     7201 gcgaacgggc acaagaacgg tcactccgat ccgaagtcgg ttctgccgtt ggaccgtgac
     7261 gagcgaggct tcggcggctt ctgagcgagc gtctcgaagc acgaatcttg ggatggtgcc
     7321 acgcgggcac tgggtacata gcgccctacg ggcactgggt acatagcgcc ctacgggcct
     7381 gggtaaacgg tgccctacgg agtaatgagt ggatagtgcc atgtggcact ggttggattg
     7441 tgccaatggg gcactgaggc aacacgatgg gtcttgaaga taacgagctc tggcaagaat
     7501 attcgaacga cggcagtgag gcgctcaaag acatcgaaga atccctgctc gttctggaga
     7561 cgaatcccaa ggataaaagc cagatcaatc gcctctaccg cgggttgcat accctgaagg
     7621 gcaactcggg attcctgcgg ctagtccaga tcgaacacct cgcccacgtc tcggaagatc
     7681 tgatcgggct ggtgcgagac cacggcgtgg tcatggacga ggcgattgtc gagctcatgc
     7741 tccgagtggt cgaccgcctg aatcaggtcg tgactcaggc gggcgaccag caccgtgacg
     7801 cggatccgag ccaagttcac gatctcacgg aagccgtcag tgagttgatg cgtcagcggc
     7861 agagcggcgc gctcgaagcg acgcctgcag cgagcgctac aaacatgagc gttgcgaacg
     7921 gtcgcgccgc gaagcccagc gaagcccccc ctttcgctga tattggtccc gtagtcgaga
     7981 gcgaagcccc gtccggagac aacatggatt ggatctgggg ggcgaacagc gtggccccga
     8041 cggcgaagat cgatcccgca gtggacgccg gttatctgga ggtcttcctc tccgtcgccg
     8101 agccggcgct caatgccatc agtgcggcga tcgcggctac gatctcgtcc gatgaagtgg
     8161 acgaggcgcg cgagaagctg aacggacaat gcgcggagct cgggctcgcc gccgagcgca
     8221 tgggctacgt gcacatcgtg gatttgctgg agaccatccg ccgcgccgcg atcgaaggtg
     8281 ggacggccct caccaatctg agtgcgctcg agatggcctt gtactcgcag ctcgtgctga
     8341 tcgagtccaa ctatcgctcg ctggtcagcg aagcgcagga cttcggaatc gtacgcagct
     8401 atcagcgggc gtgcgccaac gtcgccttgg ccgacttggc acggttacgt tacttgctcg
     8461 acgagcccga acagctcagc gagctcgagg tccgcgacct gttccaacgc ttgcgcgcgg
     8521 cctgcgaata ttatggattc acgggcgcgg cgcgcgcgtg tctcgagcgc gaggacgagc
     8581 tttgcagggt tttggagcaa tcggctcagc tcgataacga ggtgttcgct cacgccagtg
     8641 cgctgatcac cgagctcggt cagacgatct atgccgtgaa cgcgggcgaa gatctgtccg
     8701 atattccggt gcaagaggcc gccgacgatc cgatgaagtt cgatccgccc gtcttggcgc
     8761 tgccgatctc cgaggagatt cggcgtcagc tgacccgggt gggcttgtcg gcgatcggtg
     8821 atttcaaggc ccagggcgtg gctgtgctgg aggtgcgcgc cgagctggag aagcaccccg
     8881 gggtgatcga ccgcttcaac gagtggctcg gctcggggga agtctctctc gtctcgagcg
     8941 cggtcttgga ctcggagatc cccggcaacc cgcctgcgta cttgttcctg gtcggcgcct
     9001 ctgcgcccaa ggcccgctcg gcgcttggag tgctcgaccc ttcgggcaat atcttccaat
     9061 tgcgccggct cgatcagcag gaagccgagc cgccgaggcg taaaccggct ccggtcgagg
     9121 cagcgttttc gccggtgcag gagcccgtga tcttgcttgc gcccgaggcg cccccagcgc
     9181 gggccgccac gcctcagcaa gcgacgccac aggcaggccc gagccgcgcc gccgcggcgc
     9241 tcgtgcgcgg cgtatcggct agcagcaacg cccaatcgcc ggagcccagc cccggtgaag
     9301 gcgagcccga ggccagagag tcgagcgaag ccagagatcc gagcgaagcc aaagagccga
     9361 gcgaagccaa agagtcgagc gaagccagag agccgtcgaa caaggccgag ttcttgcgca
     9421 tcgacgcgcg taaggtctcg ctgatcatgg acttggcggg cgagataggg ctcgcgtgcg
     9481 gcgcggtcac ccatcacccc gatatcgagt cgatggacct cgagggcttc tctgcggctt
     9541 cacacaagct cgagatgctg attcgcgagt tgcagaacga ggtctcgggt atgcggctgg
     9601 tgcccgtcgc gggcgtcttc cagcgcatga agcgcgtggt gcgcgacgcg gcgcggcgca
     9661 cgggtaagaa ggtggagctg gtcctcgtcg gcgaggagac cgaaatcgac aaggtcatgg
     9721 ttgacagcct cgcggagccg ctggtgcaca tgctgcgcaa cgccgtagat cacgggatcg
     9781 aatccccaga cgagcggagc gcggcgggca agccggcagt gggtcgcatc gtgctcgagg
     9841 cctcgcatca gggcggggaa gttacggtgg aggtctcgga cgatggtcgg ggtttggatc
     9901 tgaaccgcat cctgtcgcga gcccgcgagc gggcgctcat tcccgccgat gccaacccga
     9961 ccgaagaaga gat
//
LOCUS       DASTKU010000986         6724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1310820, whole genome shotgun sequence.
ACCESSION   DASTKU010000986 DASTKU010000000
VERSION     DASTKU010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310820"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1648)
                     /locus_tag="VFQ61_35085"
     CDS             complement(101..1648)
                     /locus_tag="VFQ61_35085"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET9959788.1"
                     /translation="MTSFRPRASRFVLLVLAVCGVPATWTACAHDVDSTLPGSSDGGK
                     VAASSGGANHAGSSFAGTSSGGFSLGGSSSGGSSWAASGGVSSGAAAGASSSLSGSGG
                     GGSSSVGGGTSAGAGASMGGSAGATTATGGAAGAGSGGFAGASGAAGIPGLGSCVLPN
                     GANFEVVQANYEKWKATLLTADGAGGFLRVQRPNSGTQVRSSNSEGIAYGLEIAVYMN
                     DQTTFDQLWKYEQIHLGKNGLMEWEISPTGTVLGSGAASDGDEDMAFALVMAHAKWGG
                     QGTLSDTYLNYAKKQIDLIWKFEVDHSRNDVLTPGDNFADGSVINISYFAPAYYRIFG
                     QVTGKQAEWNRVAKTSYDVIEATLNAANKNQSNGLVPAWSTPAGVPMAPPGSGHPTYH
                     QLDSCRTPFRVAQDYCWFNEPRALDYLKKITAFYAAIGANNIVDGYDLDGKPHPKVNS
                     PLAAFVGGAAAGAMALPEYAKLRDESYVALAGWQTLLGDSIYYNYSWSMLSLLMLTGK
                     FVNLQPQ"
     gene            complement(1791..2543)
                     /locus_tag="VFQ61_35090"
     CDS             complement(1791..2543)
                     /locus_tag="VFQ61_35090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9959789.1"
                     /translation="MSGHSKWATIKRKKGATDAARGKVFTKITRELVTAARIAGGDPD
                     GNSRLRKAIALAKAQQMPNDNIKRAIQRGTGEIDGASFEEVLYEGTGPGGTLYLVDGM
                     TDNRNRSAAELRKVFEKHNGQMGGSGTAGWAFDRLGVITLEKSLVTEDQLMEAAIGAG
                     AEDYRDEGEVWTVYTSPNDVDRVTSAIEAAKLPVQSSKVAFVPKTKKTVSGRDAEVCL
                     NLVEALDDHDDVQNVYADFDISDEELQRIANS"
     gene            complement(2757..3230)
                     /locus_tag="VFQ61_35095"
     CDS             complement(2757..3230)
                     /locus_tag="VFQ61_35095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959790.1"
                     /translation="MSKNSRRRRLELLARCIPMLRSYVTQLSEDAESASDVFQEVILR
                     LLSEHTGPEELAEFAEHSRRVALEVAKGSLRGTAELPLDEEPLDPWLDPERAVDTRES
                     LQRAIHHLGDDALELLVRRYILGENARELSTGRSQSPAALRVRLMRLRSSARAVR"
     gene            4705..5247
                     /gene="cobO"
                     /locus_tag="VFQ61_35100"
     CDS             4705..5247
                     /gene="cobO"
                     /locus_tag="VFQ61_35100"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET9959791.1"
                     /translation="MDSNAKARTARGLLMVYTGDGKGKTTAALGMVFRALGRGLNVSV
                     IQFIKGKWKTGERLFAETQPGLRFYVMGRGFTWESEDLSLDKAAAEAAFAAAREEMLS
                     GERDLVILDELTYAFHYDFLDLESVLETLRARPPHVHVVVTGRNAPRALLDLADLVTE
                     MKMVKHPFQAGLKAQSGVDF"
     gene            5244..6668
                     /locus_tag="VFQ61_35105"
     CDS             5244..6668
                     /locus_tag="VFQ61_35105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HET9959792.1"
                     /translation="MSAEFSSPLAAQAPRPRIVLAGTSSGVGKTTLVLAIARALRERG
                     LRVALFKCGPDYLDVTYHTQALGSPAQNLDGYLMGRDAVLRTFDVASRGCDIALIEGV
                     MGLFDGREPTSNAGSTAEIASWLRAPILLVTDASGTARSLAALFRGFRDFEPGLGVCG
                     AIANRVGSSGHLKLLEQAMPGDLLGGFPREDSIAFAERHLGLCTASATTLPEEKLDRL
                     AQLATQWIDLDRLTALAREAGALPERTSESPLTKVERGATTDRRPRIGLARDAAFHFY
                     YADNLQRLEDLGATLVPFSPLEATRLPELDGLYIGGGYPELHARTLSENAELRAEIAA
                     FAERGGPIYAECGGLMYLTKAIHTIEGDRYPMVGLFDTETHQRDKLQALGYVEVETRE
                     DSLLGPRGTRFRGHQFRYSQLDREPERPLYSLRRRRGEAILEGFQVKRAVASYVHAHW
                     ASNPGVARSFVQSCRDFANAEVTC"
BASE COUNT         1210 a         2205 c         2032 g         1277 t
ORIGIN      
        1 tgcctggccg aacacaccat gcaggcaacc agtgccggct gatggagacc agcccgtgca
       61 tgccagcagg cacggccgac tgccgggcgg ggaccgggtg ttattgcggc tgaagattca
      121 cgaacttgcc cgtgagcatg aggagcgaaa gcatggacca cgagtaattg tagtaaatgc
      181 tgtccccgag cagcgtttgc cagccggcga gagctacgta cgattcgtct cggagcttcg
      241 catattcggg tagagccatg gcgcccgccg cggcgccgcc cacgaaagca gccaaaggcg
      301 agttgacctt ggggtggggt ttgccgtcca gatcgtagcc atccacgatg ttgttggcgc
      361 cgatcgcggc gtagaacgcc gtaatcttct ttagatagtc cagcgcccgg ggctcgttga
      421 accagcagta atcctgggcc acgcggaagg gcgtacgaca agaatccaat tgatggtaag
      481 tcgggtgacc ggagccgggc ggggccatcg gcacgcccgc gggggtggac caagcgggaa
      541 ccaacccgtt gctctgattc ttgttggccg cgttcagcgt ggcctcgatc acgtcgtagc
      601 tggttttcgc gactcggttc cactcggctt gtttgccagt cacctgcccg aagattcgat
      661 aatacgcagg tgcgaaatac gatatgttga tcaccgaacc atctgcgaag ttgtccccgg
      721 gcgtgagcac gtcattgcga gagtgatcga cttcgaactt ccagatcagg tctatctgct
      781 tcttggcgta attcagatac gtgtcggaaa gcgtgccctg cccaccccac ttcgcgtggg
      841 ccatgaccag cgcgaaagcc atgtcttcgt cgccgtcgga tgcggcgccg gaaccaagca
      901 cggtacccgt gggagaaatc tcccattcca tcaaaccatt cttacccagg tggatctgct
      961 cgtacttcca gagctggtcg aaggtggtct ggtcgttcat gtaaacagcg atctccagac
     1021 cgtaggcgat gccttcggag ttggaggaac gcacctgagt tcccgaattc ggacgttgca
     1081 cgcgcaggaa tccgcccgcg ccatccgcgg tgagcagggt ggccttccac ttctcgtagt
     1141 tcgcctgcac cacttcgaag ttcgccccgt tgggcagcac gcacgagccc agccccggga
     1201 tccctgccgc gccgctcgcg ccggcgaagc ctcccgaacc agctcccgct gcgccgccgg
     1261 tcgcggtcgt ggctcccgcg ctcccgccca tgctagcgcc tgctccagcg ctcgtgccgc
     1321 ctccgaccga cgagctgccg cctccgccgg aaccactcag agagctgctc gcgccagcag
     1381 cagccccgct cgacacaccg cccgaggccg cccagctcga gccgcccgag ctcgagccgc
     1441 ccaagctgaa accacccgag ctggtgcctg caaagctcga accggcatgg ttggcaccgc
     1501 ccgaactcgc ggcgaccttt ccgccgtctg acgagccggg caacgttgaa tccacgtcat
     1561 gagcgcacgc cgtccatgtc gcgggcacgc cgcataccgc caacaccaac aacacgaagc
     1621 gagaggcccg tggccgaaat gaggtcaccc gtggaacata cactatccct tagggtagtg
     1681 acgatcctca tcagtgtgtc cgaggcgcgt gactcgagcc ttacgttagg cgtgcctcac
     1741 ggcgccgtga ctcatgcgcg cctccggcgc cgtgcactct cgaccagcac tcaagaattc
     1801 gcgattcgct gcaactcttc gtcggaaata tcgaagtcgg cgtaaacgtt ttgcacgtcg
     1861 tcgtgatcgt cgagcgcctc gacgagattc aggcagacct ccgcgtctcg gcccgagacc
     1921 gtcttcttcg tcttgggtac gaaggcgact ttgctggatt gaaccggcaa cttggcggcc
     1981 tcgatcgcag acgtgacgcg gtcgacgtca ttcggtgagg tgtagaccgt ccagacctcg
     2041 ccttcgtccc ggtagtcctc cgcgccggct ccgatcgcag cttccatgag ctgatcctcg
     2101 gttacgagac tcttctcgag cgtgatcacg cccaggcgat cgaacgccca acctgccgta
     2161 cccgagccgc ccatctgccc attgtgtttt tcgaacacct tgcgcagctc cgcggcagac
     2221 cggttgcgat tgtcggtcat tccgtccacc aggtagagag tccccccggg gcccgttcct
     2281 tcgtacagca cttcttcgaa gctggcgccg tcgatctccc ccgtgccacg ctgaatggca
     2341 cgtttgatgt tgtcgttcgg catctgctgc gctttggcga gtgcgatcgc cttgcgcagg
     2401 cgcgaattgc cgtcgggatc tccacctgcg atgcgcgccg cagtcacgag ctcgcgcgta
     2461 atcttggtga ataccttgcc gcgcgccgca tcggttgcgc cctttttacg cttgatcgtc
     2521 gcccacttgg aatggccgct catggtctcc tactcgggag cgacctctta gcattgagcc
     2581 ctccgaaggg aaagcccgtc gcagcggggc gcagcacgag cggcgcccct gctccttctt
     2641 ccagcgagtt tcatcgagcg agttcggttg ctgcgagcga cccggaataa gggcacggcc
     2701 cacgctcccg acgtcttact cagcccggat tggtcgccgc tccgtgcttc ccgagactaa
     2761 cgcaccgcgc gcgcagatga ccgcagccgc atgaggcgca cgcgcaatgc cgccgggctc
     2821 tgcgagcgcc cggtcgacaa ttcgcgcgcg ttctcgccga gaatatagcg gcgcacgagc
     2881 aactcgagcg catcatcgcc caaatgatgg atggcacgct gcaagctctc acgggtgtcc
     2941 accgcacgct ctgggtcgag ccacgggtcg agcggctcct catccagcgg cagctccgcc
     3001 gtgccgcgca gcgatccctt agcgacctcg agggcaacgc gacggctgtg ctccgcgaac
     3061 tcggcgagct cctccggccc ggtatgttcg ctcaagaggc gtaaaataac ctcttggaag
     3121 acgtcgctgg cgctctctgc atcttcggac aattgggtta cgtagctgcg taacatgggt
     3181 atgcagcgag ccaacaactc caaccgacgt ctgcgactgt tcttcgacat tgcttccctc
     3241 cagggcacgc ttgcaaggcc ctcgcttttt cctgaaccat gccgacggcc tccgattgcg
     3301 gagacgcgtc tcgagcaata ttcggtcacc ttcgtgcgtt ccgtgctgct cacggcaacg
     3361 gcaggcgctg agcatcacga ctctgcgtcg cgtgcacgcg ccgtcgaacc tacggcgtcg
     3421 agaagttgcg ggtccgaatc gagactgatc gaattctcgt ctcgtttcga ttcagaattc
     3481 tcattctgga ctgattgaac tgctgattgt cgttcgcgcg cgtgctgccg accaagatgg
     3541 tttccgacta acacgtttca acaggtcagc tggctatacg tggcccagct tctcgaccca
     3601 aacgccccgc ggcacctgcg cttgcacgtg acgcgccgac ttgggtctgc cgatctcccg
     3661 atccttgccg atccgccgat ctcgaagtcc gcagtgatcc tcatgcgatc attggaactg
     3721 atcatctgaa tcaatcaact aagctgctca tccagacctc tgccgaagaa aggacgtccg
     3781 cggcggaggt tgttgctcgc ccgctagaag gcgcacgagc tggactcgtc agcgctgttc
     3841 cctcaaggcg agtactctag cgcagactcg ttcaaagcgc gatgcctgtc gagagaagaa
     3901 acgcgatcgc gtgcttggag tcgacagcat ccacacgccg atcgatcgtg tcgcgatcgc
     3961 gcgtggcggc gcacgtattg cctgtggatt tccaacgttg gactttgcgt cgacgcgcga
     4021 cgaaaaacga cgtgaacgcg aaagccgaca ctctctcaag tgacgtgcga gatctcgctc
     4081 tgtccgcgcg ctcagagccc tttgccgtct atgctgccgc ctttgcaact cgcgttctcc
     4141 gcctcgcgcg aggcggagag caagcgaacg gtgaccgtgg ctcgcaccac gggacccaaa
     4201 gtgcggctca atcccactcg caaccagcgc cgcaaattgg ggctatttcc agcggtttat
     4261 accatccacg cgcgagcgaa ggacggagca tcaacccgcg gagtcatacg cgcgggcggt
     4321 catcaatcac tctcctaagt tacatcttac cgcgcgagct tgctgccctc gaatcgatac
     4381 gacgcggcct acaccgaacg gcccctccat tcggtgggtg ccgacgcagc tcagcaaaga
     4441 cgcgcacggg caccaagccc cctgccccgc gcgtgaagag tcaagcccgc gctggagaca
     4501 gggaacctac cgactctatg aacctcggcg gactcagcgt ctctcgacac taggccttgg
     4561 cacccgcgcg ctccacgagc ggagcggtct aacggccagg gtttccatgc ctgtggccgc
     4621 gatgcatttg acacgccgag cgaagctcgg ttacgagcgg ctcgtcgtat tgctgaacgg
     4681 cggcatcgat atcggaaacg caggatggac tcaaacgcga aagcacggac cgcgcgcggt
     4741 ctgctcatgg tctacacggg ggatgggaag ggcaaaacga cggccgcgct cggcatggtc
     4801 ttccgagcgt tgggtcgcgg cctgaacgta agcgtgatcc agttcatcaa gggcaaatgg
     4861 aagacgggcg agcggctctt cgccgagacc caacccggcc tgcgctttta tgtcatgggc
     4921 cgcggtttca cttgggagag cgaagacttg tctctcgaca aggccgcggc ggaggcagcc
     4981 ttcgcggccg cacgcgagga gatgctgagt ggagagcgag acctcgttat tttggacgag
     5041 ctgacgtacg cctttcacta cgacttcctc gatctcgagt ccgtgctgga aacattacgg
     5101 gctcggccgc ctcacgtgca cgtggtcgtg actgggcgta acgcgccgcg cgctctcctc
     5161 gacctcgcgg atctggtgac ggagatgaag atggtcaaac accctttcca ggcggggctc
     5221 aaggcgcaat ctggggtgga cttttgagcg ctgagttcag ctctccgctc gccgcccagg
     5281 cgccgcgccc ccgcatcgtg ctcgcgggta catcgagcgg cgtcgggaag accacgctcg
     5341 tcttggcgat cgcgcgagcg ttacgtgaac ggggtttgcg cgttgctcta ttcaagtgcg
     5401 ggcccgacta cctggatgtg acgtatcaca cgcaggcgct cggcagcccc gcgcagaatc
     5461 tcgatggcta cttgatgggg cgagacgcgg tcttgcgtac cttcgacgtc gcctcgcgcg
     5521 gctgcgacat cgcactcatc gaaggcgtga tgggcctctt cgatgggcga gaaccgacca
     5581 gcaatgcggg ctccaccgcc gagatcgcga gctggctgcg cgcaccaatc ctcttggtca
     5641 ccgacgcctc cggaaccgct cgtagcttgg ccgcgctctt ccgcggcttc cgggacttcg
     5701 agccagggct cggggtttgc ggggcgattg ccaaccgcgt cggtagctcg gggcatttga
     5761 agctgctcga gcaagccatg cccggagact tgctcggagg ctttccgcgc gaagactcga
     5821 tcgcattcgc ggagcgtcat ctggggctgt gcaccgccag cgccaccacg ttgccggagg
     5881 agaagctgga tcgcttggcc cagctcgcta cccagtggat cgatctggac cggctcaccg
     5941 cgttggcgcg cgaggcagga gctctgccgg aacggacctc ggagtcgccg ctcaccaagg
     6001 tcgaacgggg agccacgacc gatcggaggc ctcgcattgg cttggcccgc gacgccgcct
     6061 ttcatttcta ttacgcagac aatctgcagc gtctcgagga tctgggagca acgctggtcc
     6121 ccttctcacc gctcgaagcg acgcggctgc cggagctaga cggactctat attggcggcg
     6181 gatatccgga gctgcatgcg cggactctca gcgaaaacgc cgagctccga gcggagattg
     6241 ccgccttcgc cgagcgcggt ggccctatct atgccgaatg cggcgggttg atgtacctaa
     6301 ccaaagccat tcacacgatc gaaggcgacc gctacccgat ggtggggctg ttcgataccg
     6361 agacgcatca gcgcgataag ttgcaagccc tgggctacgt cgaggtggag actcgggagg
     6421 attctctact cggtccgcgt ggcacccgct tccgcgggca ccagtttcgg tattcacagt
     6481 tggatcgtga gcccgaaagg ccactctact cgctgcgccg ccgtcgcggg gaggccatcc
     6541 tcgagggctt ccaagtgaag cgagcggtgg cctcgtatgt ccacgcgcac tgggcctcca
     6601 atccgggtgt ggctcgctcg ttcgtgcagt cgtgccgcga cttcgcgaac gcggaggtca
     6661 catgctgacc gcgcacctcc ccacccagcc cgtgtggctc gcgcgctctt ccgccgggtc
     6721 aatc
//
LOCUS       DASTKU010000987         3951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1535115, whole genome shotgun sequence.
ACCESSION   DASTKU010000987 DASTKU010000000
VERSION     DASTKU010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535115"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFQ61_35110"
     CDS             complement(<1..185)
                     /locus_tag="VFQ61_35110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959793.1"
                     /translation="MAPRSSGRSSEWLRRVRRRLTGRKFAVFFSVIWAVGCERGDAPR
                     DFKLELPETLLSTDPVRV"
     gene            complement(456..2477)
                     /locus_tag="VFQ61_35115"
     CDS             complement(456..2477)
                     /locus_tag="VFQ61_35115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HET9959794.1"
                     /translation="MQHAHSHVPPADYPSPLPWLGRKGRVASLDFPDREATAAIPPQR
                     EPVRLKILNQPARPTELPASAGAPARAEEPARRAEEPAKPSSDAKVAERKPAPPKKPG
                     SKRPGSGIPARPIIRRLVWARRVAQVAFFGLFFWFLCSTAFRGTFSAKFDQPVRLPYP
                     VEAFLLADPFVGAMTLLSTHTVYRGLAWSLGILALTLVFGRVFCGWICPFGTLHHFFG
                     WIFPSRYLRGNKRVEANRTHGWQAGKYYLMWAFLGAAVAGSAIGGLLDPICVAVRAIG
                     LGVLPALQYLGIRSTNAVADTNIRALQYGMDSVQDGLSRTLFTPNQAYFNQTWFIVFL
                     LVAILFMNRIVPRFWCRALCPLGAFLGVISRFSLFGMEKDHAKCTDCNLCLVHCQGAD
                     SPQGGVKHRKDECHMCMNCEAACPEDVIKFRFLPGLRSIQKKPDLERRTVVATAAAGV
                     VAVPALRIANWPDKAYSEKVIRPPGSVEEREFLERCIRCAECMKVCPNNALHPAFFEA
                     GIEGLWTPILIPRIGYCEFSCVLCGQVCPTGAIQKIDEKQKMGINQKPVTIGTAMYDH
                     GRCLPWAMATPCIVCEEFCPTSPKAIWAEEVEIPKRDDKYKAKGEGAAMLTVKVQRPH
                     VDPSLCVGCGACEKVCPIVDKPAVYVTNAGESRSRTNVILLENTGYGKT"
     gene            complement(2479..3450)
                     /locus_tag="VFQ61_35120"
     CDS             complement(2479..3450)
                     /locus_tag="VFQ61_35120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HET9959795.1"
                     /translation="MSDRPSRREVIQRVALTGAVVSGSTLGAGLLYDPGGFELSRPAG
                     ERQVRDFRVRANGPAPVYAVAKSSQEPEILVQRAVAALGGMKRFIARGDIVAIKPNIG
                     WDRMPVHAANTNPRVVAEVVRLVFEAGAKQVIVTDASCNEPNRCFQRSGIWRAAYGLG
                     ATVILPSAHRFREMRMRGDVLDQWPIYTPLVNADKVINVPVAKHHNLSRYTAAMKNWY
                     GVLGGRRNRLHQNIDVSIADLATFMRPTLTIVDATRVLLRNGPQGGNIDDAKDLHQVI
                     ASLDQVAADAYGCTLIGEKPERIRYLKLGHERGIGNMNFDAVPHVEV"
     gene            3785..>3951
                     /gene="rpmE"
                     /locus_tag="VFQ61_35125"
     CDS             3785..>3951
                     /gene="rpmE"
                     /locus_tag="VFQ61_35125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET9959796.1"
                     /translation="MKDGIHPEYEVVNVSCACGNTFQTRSTKKELGVDVCGACHPFYT
                     GKQRLVDTQGRV"
BASE COUNT          743 a         1289 c         1239 g          680 t
ORIGIN      
        1 acgcggaccg ggtccgtgga cagcagcgtc tcgggcaact ccagcttgaa atcacgcggc
       61 gcgtcgccgc gctcacagcc cactgcccag atcaccgaga aaaataccgc aaacttgcgc
      121 ccggtcagcc gccgtcgaac acgccggagc cactcactcg aacgcccact cgaacgcgga
      181 gccatcgccg tagaacctcg ccagcccgac gctcgtatgc gcgggctcga tcggcgagcg
      241 tacaccagtc cccgactggg tcacatctcg aagctcgtga gcgagtttcc ggcggccgcg
      301 taacaacgtg cgcgctcctt cggcgatgcg cccccaagcg caaccccgct tccaagcttt
      361 ctcacccatc accgcgccgg cccccccgga acgaacgctc actccattca cgcgcggatg
      421 aggttaaacc cgcacggatt tcaacgcgcg cagacttacg tctttccgta acccgtgttc
      481 tcgagcagga tgacgttggt tcgcgaccgc gactcccccg cgttcgtaac gtaaacggca
      541 ggcttgtcga cgatcgggca gaccttctcg caggcaccac agccgacgca cagcgagggg
      601 tctacgtggg gtcgctgcac cttgaccgtg agcatcgcgg cgccctcacc cttggccttg
      661 tacttatcgt cgcgcttcgg aatttcgacc tcctcggccc aaatcgcctt cggagaggtc
      721 ggacagaatt cctcgcacac gatgcagggg gtcgccatcg cccacggcag acagcggccg
      781 tgatcgtaca tcgcggtgcc aatcgtgacg ggcttctggt tgatgcccat cttctgtttt
      841 tcgtcgatct tttggatggc accagttggg cagacttggc cgcacagcac gcaagagaac
      901 tcacagtagc caatgcgcgg gatgaggatg ggcgtccaca ggccctcgat gcccgcctcg
      961 aagaaggccg ggtgaagcgc gttgttgggg cacaccttca tgcactcggc gcagcggatg
     1021 caccgctcca agaactcgcg ttcttccacc gagcctggcg ggcgaatcac cttctcgctg
     1081 taggccttgt caggccagtt cgcgatgcgc aacgccggca cggccacgac ccccgccgcc
     1141 gcggtcgcga cgaccgtgcg ccgctcgaga tcgggcttct tttgaatact gcgaaggcca
     1201 ggcaagaacc ggaacttgat cacgtcctcc gggcaagcgg cctcgcagtt catgcacatg
     1261 tggcactcgt ctttgcggtg cttgacgcca ccttgcgggc tatccgcacc ctgacagtgc
     1321 accaggcaca ggttgcagtc cgtgcacttg gcgtggtctt tctccatgcc gaacaaggag
     1381 aagcgcgaga ttacgccgag aaacgcgccg agcgggcaga gtgcacggca ccagaagcgc
     1441 ggcacgatcc gattcatgaa caggatcgcg accaacaaga agacgatgaa ccaagtctgg
     1501 ttgaagtagg cttggttggg ggtgaacagc gtgcgcgaga ggccgtcctg gacggaatcc
     1561 atcccgtact gcagcgcacg tatgttggtg tccgcgaccg cgttggtgct gcggatcccg
     1621 aggtattgca gcgccggcag caccccgagc ccaatcgccc gcacggccac gcaaatcggg
     1681 tcgagcaagc caccgatggc gctgcccgcg acggccgcgc ccaggaaagc ccacatcaag
     1741 tagtacttgc cggcttgcca gccatgcgtg cggttggcct ccacccgctt gttgccccgc
     1801 agatagcggc tcgggaaaat ccagccgaag aagtggtgaa gcgtcccgaa cgggcagatc
     1861 cagccgcaaa atacgcgacc gaacacgagc gtgagcgcga ggatcccgag cgaccaggcc
     1921 aagccccggt aaaccgtgtg ggtgctgagc agagtcatgg cgcccacgaa cggatcggcg
     1981 agcaagaacg cttcgaccgg gtacggcaac cgcacgggct ggtcgaactt ggcggagaac
     2041 gtgccgcgaa acgcggtgct gcacaaaaac cagaagaaca gcccgaaaaa ggcgacctgc
     2101 gcgacacgcc gggcccagac caggcgccgg atgatcggac gtgcggggat ccccgacccc
     2161 ggccgtttgg atcctggctt tttgggtggt gccggtttgc gctcggccac cttggcatcg
     2221 ctcgaaggct tggctggttc ttcagcacga cgagcgggct cttccgcacg tgccggcgcg
     2281 ccagcgctgg cgggcagctc ggtgggcctc gcgggctggt tcaggatctt gagtcgtacc
     2341 ggctcgcgtt gcggtgggat cgcggcggtt gcctcgcgat ccggaaagtc gagcgacgca
     2401 acccggcctt tacgaccgag ccagggcagc ggcgacggat aatctgccgg tggcacgtgc
     2461 gagtgcgcgt gttgcatctc acacctccac gtggggaacg gcgtcgaagt tcatgttgcc
     2521 gatgccccgt tcgtgaccga gcttcagata acgaatccgc tccggcttct caccgatcag
     2581 cgtgcagccg taggcatccg ccgcgacttg atcgaggctc gcgatcactt ggtgcagatc
     2641 cttggcatcg tcgatgttgc cgccttgggg gccattgcgg aggaggaccc gcgtggcgtc
     2701 cacgatcgtg agcgtggggc gcatgaaagt cgccaagtct gcaatcgaca cgtcgatgtt
     2761 ctggtgcaag cggttgcgcc gcccgcccag cacgccgtac caattcttca tggccgcggt
     2821 gtagcgagac agattgtgat gcttggccac cggcacgttg atgaccttgt ccgcgttgac
     2881 gagcggcgtg tagattggcc attgatcgag tacgtcgccc cgcatgcgca tctcgcgaaa
     2941 gcgatgggcc gacggcaaga tcacggtcgc accgagcccg tacgccgcac gccagatccc
     3001 cgagcgctgg aaacaacgat tgggttcgtt gcacgatgca tcggtgacga tgacctgttt
     3061 ggctcccgcc tcgaacacca agcgcaccac ctcggctacc acgcggggat tcgtgttcgc
     3121 ggcatgcacg ggcatgcgat cccaaccgat gttgggcttg atggccacga tgtctccgcg
     3181 cgcgatgaag cgcttcatgc cgccgagcgc ggccaccgcg cgttgcacca agatctctgg
     3241 ctcttggctg ctcttggcga cggcgtacac gggggccggg ccgttcgctc gcacgcggaa
     3301 gtcgcgcact tggcgctccc ccgcaggacg cgacagttcg aaaccgccgg gatcgtaaag
     3361 caagccagcc ccgagcgtcg agcccgagac aactgctcca gtgagcgcga cgcgctgaat
     3421 gacctctcgg cgtgacggcc ggtccgacat ggttccggga acttgttcga gggctgacgt
     3481 aacgtcaagt ttcgcggtct tatcgcgtac gcagccacat aactccaacg atctcagaaa
     3541 cctgcgcgag tgtccattgg ggtgtctgcc cgacgcgcct ctgcctcgga cggcctcgcc
     3601 gagcgcacga tttatgatcc ggccgcttgt cgaaagggga caccggcgct agcctccagc
     3661 tccttttatc ggcgcctgcc gcgtcgaaga agctcgggcc gccgctgcag acgcgacggc
     3721 cagtcgttag caatgtccgc aaggtcgctc gaagatcgct ctcgaagatc gctggagtaa
     3781 agtcatgaag gacgggatcc acccggagta tgaggtcgtc aacgtgagct gcgcgtgtgg
     3841 aaacacgttc cagactcgct ccaccaagaa ggagctcggg gtcgacgtgt gtggcgcttg
     3901 ccatccgttc tatacgggca agcagcgcct ggtcgacacg caaggccgcg t
//
LOCUS       DASTKU010000988         8359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_862659, whole genome shotgun sequence.
ACCESSION   DASTKU010000988 DASTKU010000000
VERSION     DASTKU010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862659"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VFQ61_35130"
     CDS             <1..690
                     /locus_tag="VFQ61_35130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959797.1"
                     /translation="QMLAYPGNRNFAAGLVDYLVEDDAWGPRGGSLYILTNRFRQTGR
                     YGKADGLSDELTQTARNLAESLDGWHRDGLPSSVARALGGAAALTALLLLALTSLRRY
                     QRATPSYAAPISALAQGGLSGRAAVLSAPTTDRALVVAELTELLREELVRMAGLPVLS
                     SHQAALAEFEKRGVPKAWLTRCREALKFGELAENALRAQRRSGISVGQVTDQERRVTE
                     LLVDLERSGHK"
     gene            706..1674
                     /locus_tag="VFQ61_35135"
     CDS             706..1674
                     /locus_tag="VFQ61_35135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET9959798.1"
                     /translation="MVALQVDEIQAARERLTAVEREVSRVYIGELSAIDTLLVALLAR
                     GHVLLEGVPGIAKTTLVKAFAGALGCSVRRVQFTPDLLPADITGTYVLNPKDGTFSLK
                     AGPIFTHVLLADEINRAPAKTQSALLEAMQEHQVTIEGDRFALPDPFFVLATQNPIDL
                     EGTYPLPEAQIDRFLVRVQMGYPSVSDEVAMLRAHGVRPPEVKPLLSSTEVQTLQSIA
                     ERVHVEEDLMEYAVALSRFTRENTRVVLGASPRASLSLLRAAKAKALIDGQGFATPDH
                     VRAMAVPVLAHRLVLVPDLDSEPRARQKIVEEALSRVSYRRAARHS"
     gene            1774..3252
                     /locus_tag="VFQ61_35140"
     CDS             1774..3252
                     /locus_tag="VFQ61_35140"
                     /inference="COORDINATES: protein motif:HMM:NF012754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959799.1"
                     /translation="MARIRIDRGTVYTGLSLAPLILLSCSSNGEAPPGAVIDAGGAVS
                     GGSANTTSSGGITHTFVNTSSNTGAVTSGGAVTNGGASTSGGAGNVSSGGSFAPVGGS
                     QDASGGKPSSSGGSSSTAAGGRETTGGRNSISGGAAAQGGRSQGGGVNSGGELASGGK
                     ASGGSTASAGAPSGTAGASSCPSDLLGWATVNGDGVNTTTGGGNAAAVRPTTPQQLMD
                     YASDDAPRVIEVAGNFAVPRLQVNSNKTLIGIGKDATINGGVRIRGSSSEPVSNVIIR
                     NLRVNGATTQVDNDAMQIYFAHHVWIDHSEIWDGPDGNLDMTHAVNWVTVSWTKFRYT
                     TAYQRPSGEDADHRFSTLIGHSDNNSSEDTGRLKITFHHNWWAERVLERMPRVRFGQV
                     HVFNNYFSSAGNNYCVRAGVGAALLVENNFFDGVKSPHEFNSDEDQKTANITVRGNVY
                     QNTSGVQATGGGGPAFTSAPYPTNMDAANGIKDLVQSCAGPR"
     gene            complement(3372..4319)
                     /locus_tag="VFQ61_35145"
     CDS             complement(3372..4319)
                     /locus_tag="VFQ61_35145"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET9959800.1"
                     /translation="MDVLTDVLETVRARAACSGRVDALAPWGMEFAEGEAARFHVVLE
                     GSLILQAEPVGTLALHKGDLVALPHGDRHVLSDTADSPVLPLEEWKRRAGNQDGVRGA
                     LPIGGVGARTALWSGRIEFEDGRGNPLLSVLPRVIVLRGEWSRSVQSLEPTLGMLASE
                     SISGRPGAQTIVSRLADVIFVQIVRGYLATLGSEGTGWLAALSDPQVGAALAWVHREP
                     ARDWTVQELAQRVAMSRSAFAARFTRMVGEPPLHYVTRWRMQKAASLLREGRSTIAQI
                     AESVGYESEAAFSKAFKRAVGSAPGAYRRAARPDLDAAA"
     gene            complement(4601..6358)
                     /locus_tag="VFQ61_35150"
     CDS             complement(4601..6358)
                     /locus_tag="VFQ61_35150"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HET9959801.1"
                     /translation="MRARRSAALSLACALFFALLARVERAKAELPTYSPFEQSVIAEE
                     LDKKQGQIEATPEGKRIESIEIARRDVLDERDPIPDIFNFFHVTTREHVIRRELLFRE
                     GQRFDWARVDETARNLRGLPQLSLVLIVPLTGSSPDRVRLLVVTKDVWSLRLNSNFQY
                     GPEGLTYLFLNPTEWNLFGTHAMIGGLYSLRRSTYSLGLSLSHARIMGTRLAGSAALS
                     LVFERKTSQAEGFIGVFNYGLPQYSARQRYFYGTALNLEDGIVRVSTQSAQDNQPILL
                     SYHRERYVARTSATRSFGLVRKNDLTLGIEADRRVYRSRVPGDVDPALRSEFESKEIP
                     VSDTRVSPYLKLTHYENRFLKTIELETLGLQEDFRLGAELQTKVYVAGQRFGSSRNLL
                     GVFSGVSYTVALGNGLVRGLVSSSIEYADSARHQALATAALRIATPRLGPGRFILDGV
                     VENRFYNYLRNRSALGGEGRLRGYPASDLEAKGSSSQRGSDAMAFNAEFRSRAIEILS
                     AQCGMAAFFDMGAAAASMRQLSFRRSAGAGFRVLFPQADRLVLRLDWAVPFDDPRGKL
                     PGAVFLTFGQAFTMPDFTN"
     gene            6581..8179
                     /locus_tag="VFQ61_35155"
     CDS             6581..8179
                     /locus_tag="VFQ61_35155"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET9959802.1"
                     /translation="MQIHPTRSAVHLCVAGSLAVLCGVALDVPAVIAWAGAIVVGLGV
                     ARAVTWVSVARIRTAGFEMLWRGSPRVRRLARRDELMLEAELRNRDVRAARFVDLRPV
                     ASPDLEVEVKPTQGEVPAGGRISVELRVSARRVGYHAIHGLSLELEAGPGLFEVPLTF
                     ANPLGLEVLPKPHTLTAHSARGARSRRMADLGRARPLSGESSELRELREHQAGDPFRR
                     IAWKASARRGRLLVREYEREEQDVVFILLDAAIELYAGKPGQAPLDRAIDEVASLAIR
                     HLNRGDPVGLGVIGRRALAWLPPKRGAAQIARILEALAHSTSPVHADRSALEEGEVAL
                     RVLEHMRPLDPEVAGNVGPYELDRIARRADKLKPRAPFPAATPFAPTPRERSLRSYLW
                     AFGLGSQARTGPERLEVDEQLIRALSRARAERPQPSLVYLCTPAPEGESRRRLVDEAA
                     KKRGQRSSLIWLNVPSYAGFNSGPGFESVVARTLLRRAQNMNRVGERTLRQRGIQVAH
                     LSGEDEREKVAEETQSDVAASGLG"
BASE COUNT         1605 a         2681 c         2660 g         1413 t
ORIGIN      
        1 caaatgcttg cgtaccccgg caatcgaaat tttgccgctg ggctagtgga ctatctcgtc
       61 gaagacgatg cgtggggccc gcgcgggggc tcgctgtaca ttctcaccaa ccgtttcagg
      121 cagaccgggc gttacggcaa agctgatggc ctgagcgatg aactcaccca aacggctcga
      181 aatctggccg agtcgctcga cggttggcat cgggacgggc tcccgagcag tgtcgcacgc
      241 gctctcggtg gagcggctgc gttgacggct ctattactgc tggcgctgac ctctctccga
      301 cggtaccagc gcgccacgcc gtcgtacgcg gcaccgatct ctgctttggc tcagggcggg
      361 ctgtctggac gcgccgcggt gctgtcagca ccaacgaccg atcgcgccct cgtcgtcgca
      421 gaactcacgg agctgttgcg cgaagaactc gtgcggatgg cggggcttcc cgtcctctct
      481 tcgcaccagg cagcgctggc ggagttcgaa aaacgtggcg tgcccaaggc gtggttgacg
      541 cgctgccgtg aagctttgaa attcggcgag ttggccgaaa acgcgctgcg agcgcagcgc
      601 cgcagcggca tcagcgtcgg ccaagtgacc gatcaggagc gtcgcgtcac cgagttgctt
      661 gtggatctag aacgatccgg gcacaagtag gaccctctcc aaagcatggt cgcgctgcaa
      721 gtcgacgaaa ttcaggccgc ccgtgagcgc ctcaccgccg tggaacggga ggttagccga
      781 gtgtacatcg gtgagctttc cgcaatcgac acgctgttgg ttgcgttgct cgcccgggga
      841 cacgtgctgc tcgaaggcgt acctgggatc gccaaaacga ctttggtgaa ggcattcgct
      901 ggcgcgctcg gctgcagcgt gcgccgcgtc cagttcaccc ccgacctact gcccgcggac
      961 attacgggaa cctacgtcct gaatccgaaa gacggcacgt tttccctgaa agcgggcccg
     1021 atcttcaccc atgttttgtt ggccgacgag atcaatcgag cccccgccaa aacccagtcc
     1081 gcgttgctcg aagcaatgca agagcaccag gtgacgatcg aaggggatcg gttcgccctc
     1141 cccgatccat tcttcgtgtt ggcgacccaa aacccgatcg atctggaggg cacctatccg
     1201 ctgcccgaag cccaaatcga tcgcttcctg gtgcgggtgc agatgggtta cccgagcgtg
     1261 agcgatgaag tggcgatgct gcgcgcccac ggcgtgcggc cgccggaagt gaagccgctc
     1321 ctgagctcga cagaggtgca gacgctgcag tcgatcgcgg agcgggtcca cgtcgaagag
     1381 gacttgatgg agtacgcggt cgcgctgtcg cgattcacgc gagagaacac gcgagtcgta
     1441 ttgggggcct ccccgcgcgc gtctttgtcc ttattgcgtg cggccaaggc caaggccttg
     1501 atcgacggcc aagggtttgc cacgccagac cacgtgcgtg ccatggcggt gcccgtcctc
     1561 gctcatcggc tcgtcttggt tccggatttg gacagcgagc cccgagcccg tcaaaaaatc
     1621 gtagaagaag cgctgtcgcg ggtaagctac cgacgcgcgg cgcgccactc ctgacatcat
     1681 catccgcgag tcttgcgcgc ggcaggcgcg cacccccagc gtctccggca aaaatgtgac
     1741 ccgcagtgcg tctgcggcga cacgagacca gggatggctc gcattcgcat cgaccgaggc
     1801 acggtttaca caggtctcag cctggcaccg ttgatactgt tgtcttgcag ttcgaacggc
     1861 gaagcgccgc ccggagccgt gatcgacgca ggaggcgccg tgtcgggtgg gagcgccaac
     1921 acaacttcga gcggcggcat cacccacacg ttcgtcaaca cctcttcgaa cacaggcgct
     1981 gttacgagtg gtggtgctgt tacgaacggt ggggcttcga cgagcggcgg cgcggggaac
     2041 gtaagcagcg gaggtagttt cgcaccagtc ggcggttcgc aagacgcgag tggtgggaag
     2101 ccgtccagct ccggcggctc gagcagcact gccgcgggcg ggcgtgaaac taccggaggg
     2161 cgcaactcga tcagcggagg agcagcggct cagggcgggc ggtcccaagg tggcggcgtc
     2221 aactcgggcg gcgaactcgc cagcggcgga aaggccagcg ggggaagcac cgcgagcgcc
     2281 ggagccccga gcggtacagc cggcgcgtcc agctgcccca gcgatctgtt gggctgggcg
     2341 acggtgaacg gggacggcgt caacaccact acgggcgggg gtaacgccgc cgccgtgcgt
     2401 cccacgacgc cacaacagtt gatggactac gcgagcgacg atgcgccgcg cgtgatcgag
     2461 gtcgcgggca attttgcggt gccgcgtcta caagtgaact cgaacaagac cctgatcggt
     2521 atcggcaaag acgcgacaat caacggtgga gtccggatcc gtggcagcag cagcgagcca
     2581 gtgagcaacg tgatcattcg caacctgcgg gtcaatggcg ccaccaccca ggtggataac
     2641 gacgcgatgc agatctattt cgcgcatcac gtctggatcg atcattcgga gatctgggac
     2701 ggccccgacg gcaacctcga catgacccac gccgtgaact gggtgaccgt ctcgtggacc
     2761 aagttccgct acacgacggc ctatcagagg ccttccggag aagacgccga ccaccgcttc
     2821 tccactctga ttggccactc cgacaacaat tcatccgaag acacggggcg gctcaagata
     2881 actttccacc acaactggtg ggcggaacgc gtgctcgagc gcatgccgcg cgtgcgcttc
     2941 ggtcaagtgc acgtcttcaa caattacttc tcttctgccg gcaacaacta ctgcgtcaga
     3001 gccggagtcg gagcggcgct gctggtcgag aacaacttct tcgacggcgt gaagagcccg
     3061 cacgagttca actccgatga agatcagaaa acagcgaaca tcaccgtccg cggcaatgtt
     3121 taccagaaca ccagcggcgt gcaggccacc ggcggtggcg gccccgcgtt cacgagcgcc
     3181 ccgtacccca cgaacatgga cgccgcgaat ggcataaagg acctcgtgca aagctgcgcg
     3241 ggtccacgtt gacgccaaac ccaaagatcg aaaaaaaacg agcccgtgcc agctacaccg
     3301 acacgggctc tcgattgagc tcagccaagc ctgttcgctc gtgcgcagtc agggcgctgc
     3361 aggagtgttc cctacgccgc ggcgtccaag tcaggacgcg ccgctcgacg ataagcaccc
     3421 ggggcgctac ccaccgcgcg cttgaacgcc ttgctgaacg ccgcctcgga ctcgtaaccc
     3481 accgactccg cgatctgcgc aatcgtgctg cgtccctcgc ggagcaagct cgccgccttc
     3541 tgcatacgcc agcgagtgac gtaatgcagc ggaggctcgc ccaccatgcg agtgaagcgc
     3601 gcagcaaagg cggagcgcga catggccacg cgctgcgcga gctcttgcac ggtccagtct
     3661 ctcgcgggtt cgcggtgaac ccaggcgagc gcggctccca cctgcggatc cgagagcgcc
     3721 gcgagccagc ccgtgccttc actgccgagg gttgcgagat agcctcgcac gatctgcacg
     3781 aagatcacgt ccgccaagcg gctgacgatg gtctgggctc cggggcgccc cgagatactt
     3841 tcggaagcca acataccaag cgtcggttcg agtgattgca ccgaacgcga ccactcgccg
     3901 cgcagcacga tcacccgggg cagcacggag agcaaaggat taccgcgccc atcttcgaac
     3961 tcgatgcgac cactccacag agcggttctc gcaccaactc ctccgatggg gagagcaccg
     4021 cgcacgccat cctgattgcc tgcgcggcgt ttccattctt cgagcgggag cacgggacta
     4081 tcagcggtgt cggagagcac gtggcgatca ccatgaggta acgcgacgag atcacctttg
     4141 tggagagcca aggttccaac aggttccgcc tggagaatca ggctaccttc gagcacaacg
     4201 tggaacctcg cggcctcgcc ttcggcgaac tccatacccc aaggcgccag ggcatcaacc
     4261 cgccccgaac acgccgcccg cgcacgaact gtttcgagca cgtcggttag tacatccatt
     4321 tcgtcagttt tttacgcaac gcccgaactc cgagtttcat cagaaactca ctatttcgtt
     4381 cggcctcgcg gccgtaaatc acgagttcgg tcgcgcacag ccgacacgca gttttgccat
     4441 cctcacagtc gcgtcgtcac ggccaccacc ggttgcgcag tgaactcgat tttagagcga
     4501 tccaggcgac acacagccaa gtgatcacgg gtcgcgaccc cctgaaggtt cgccgtcacg
     4561 ttcccccgtt gtcgaacccg ttagtgcgga tcccactgac tcagttcgtg aagtcaggca
     4621 tagtgaaagc ttgcccgaac gtgaggaaca ctgccccggg cagcttgccg cgggggtcat
     4681 cgaacggtac cgcccaatcc aggcgtagaa ccaagcgatc ggcctgggga aacaagacgc
     4741 gaaagcccgc tcctgcggag cgccgaaacg agagctggcg catggacgcg gcggcagcgc
     4801 ccatgtcgaa gaacgccgcc attccacatt gagcggagag gatctcgata gcgcgcgagc
     4861 ggaactcggc gttgaacgcc attgcatctg agccacgctg ggaactgctg cctttcgcct
     4921 cgagatccga agcagggtag ccccgaagac gcccctcccc acccaaagcc gagcggttgc
     4981 gcaggtagtt gtaaaaacgg ttctcgacga ccccgtcgag aatgaagcgg cccggcccca
     5041 gccgaggcgt tgcaatgcgc agcgccgccg ttgcaagtgc ttggtgccgg gcagaatcgg
     5101 cgtactcgat cgaggatgac acgagccccc gaactaaccc attaccgagc gctaccgtgt
     5161 aagacacacc ggagaagacg ccgagcaagt tacggctcga gccgaagcgc tgccccgcga
     5221 cgtaaacctt cgtctgcagc tctgccccga gccgaaagtc ctcttgaagc ccgagtgtct
     5281 cgagctcgat ggtctttagg aagcggttct cgtagtgcgt cagcttcaaa tatgggctga
     5341 cgcgtgtgtc actgacgggg atctccttgc tttcgaactc gctgcgcaga gccggatcga
     5401 cgtcccccgg aactcgggag cgatacacac gacgatccgc ctcgatgccc aacgtgagat
     5461 cgttcttgcg aaccaacccg aacgaacgag tggccgaggt gcgggccacg tagcgctcac
     5521 gatgatagct gagcaggatc ggttggttgt cttgcgcgct ttgcgtgctc acacgcacga
     5581 taccgtcttc caggttcagc gcggtgccat agaagtagcg ttgccgcgcg gagtactggg
     5641 gcaggccgta gttgaacacg ccgatgaacc cctcggcctg cgaggtcttc cgctcgaaca
     5701 ccaagctcag cgccgcggaa ccggccaagc gtgtacccat gatgcgggcg tgcgagagcg
     5761 atagaccgag cgagtaagtg ctgcgcctca acgagtacag tccgccgatc atcgcgtggg
     5821 tcccgaacag gttccactcg gtggggttca ggaacaggta cgtgagccct tcggggccgt
     5881 actgaaagtt cgagttcaga cgcaagctcc agacgtcctt cgtcacgacc agcagacgca
     5941 cgcgatcggg gctcgatccc gtaaggggca cgatcaggac cagcgacagc tggggtaagc
     6001 cgcgtaaatt gcgcgcggtc tcatcgacgc gcgcccaatc gaagcgttga ccttcgcgga
     6061 acaggagctc gcgccgaatc acgtgttcgc gcgtagtaac gtggaagaaa ttgaagatgt
     6121 cgggaatggg atcgcgttcg tcgagcacgt ctcgccgtgc gatttcgata ctctcgatcc
     6181 gcttcccctc tggcgtcgcc tcgatttgcc cctgcttctt atcgagttcc tcggcaatca
     6241 ccgactgctc gaacggcgag tacgtgggta gctcggcctt ggcccgctcc acacgcgcga
     6301 gcagtgcaaa gaatagcgcg caggccagtg acaacgcagc cgagcgacgg gctctcatcg
     6361 cgcgaagggt cataatccga agcaggcctt ggctttcttc gccaggctcg ttggggactc
     6421 gggtcggcgc acgtgggcga ggatacaacg tggaacgcgc gcgagaacac cacgccgaac
     6481 acgttgcctc ttgcgcgggt aaggccggcc aacctgcttt cgaacgagag aacgcgcgga
     6541 agcaggtgca tcggcgcagt gcacggacta ggtttggccc gtgcagatcc acccgacccg
     6601 ttcggcggta cacctgtgcg ttgcggggag cctcgccgta ctgtgcgggg tggctctgga
     6661 tgtgcccgcg gtgatcgcct gggcgggagc gatcgtagtc gggctcggtg tcgcgcgcgc
     6721 cgttacgtgg gtcagcgtcg ctcggatccg caccgctggg ttcgagatgc tttggcgggg
     6781 ctcgccgcgc gtacggcgtc tcgcacggcg agatgagctg atgctggaag cggagctccg
     6841 caatcgcgac gttcgcgccg cccgcttcgt cgacctacgc ccggtcgctt ctcctgacct
     6901 cgaggtggaa gtgaagccca cgcaaggcga ggtgccggcg ggagggcgta tctcggtgga
     6961 gctgcgagtt tcggcccgtc gcgtgggcta tcacgccatc cacgggctct cgctagagct
     7021 ggaggcgggc cccggtctct tcgaggtacc gttgacgttt gcaaacccgc tggggttgga
     7081 agttttgccg aagccccaca cccttaccgc acatagtgct cgcggcgcgc gcagtcgacg
     7141 catggccgat ctcggacgcg ctcgccccct ctccggggag agctccgagc tgcgtgagtt
     7201 gcgtgaacat caagcagggg atccgttccg acggatcgct tggaaagcat ccgctcggcg
     7261 cggccgcttg ttggtccgcg aatacgagcg ggaggagcag gatgtcgtct tcatcctgct
     7321 cgacgcggcc atcgagctct atgccggcaa acccggacag gcgccgctcg atcgagcgat
     7381 cgacgaggta gcctccttgg cgattcggca cttgaaccgc ggggatccgg tcgggctcgg
     7441 cgtcattggg cgtcgcgccc tggcgtggct acctcccaag cgaggggcgg cacagatcgc
     7501 gcggatcttg gaggcgctcg cgcactcgac gagcccagta cacgcggatc gtagcgctct
     7561 cgaggaaggc gaagtggcgc tgcgggtgct cgaacacatg cgcccgctcg atcccgaggt
     7621 cgcgggcaac gtcggtcctt acgagctcga ccgaatcgcc cgtcgtgcgg acaagctcaa
     7681 gccgcgggcg ccatttccgg ctgcaacccc cttcgcgccg acgccgaggg agcgttcatt
     7741 gcgaagttat ctgtgggcgt tcgggctcgg ctctcaggcg cgcacggggc cggagcgact
     7801 ggaagtcgat gagcaactca ttcgagcact gtcccgggcg cgcgccgagc gaccacagcc
     7861 gagcctagtc tatctctgca ctcccgcccc cgaaggggag tctcgaagac gcttggtgga
     7921 cgaggctgct aagaaacgcg gccaacgttc gagcctgatt tggctcaatg ttccctccta
     7981 tgcgggcttc aactcgggac ccggcttcga atccgtggtg gcacgcactc tgctcagaag
     8041 agcgcaaaac atgaaccgcg tcggcgagcg cacgctccgc caacgcggaa tccaagtggc
     8101 ccatctgtcg ggcgaagacg agcgcgagaa agttgccgag gagacccagt cggatgtggc
     8161 ggcgagcgga cttggctgac atcccctacg tctgaatctt cgcgcacccg ttaccaagct
     8221 aacgcgcggg caccacttcg aagcgacggg accactgctc cccttcatcc acgcgcaccc
     8281 gcagctgcca tgtaccgggc gcgtcgccgc gacgaaaggc cagcttggtc tcggcgcggc
     8341 gctcccctgg ataggcccg
//
LOCUS       DASTKU010000989         5034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1311079, whole genome shotgun sequence.
ACCESSION   DASTKU010000989 DASTKU010000000
VERSION     DASTKU010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311079"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFQ61_35160"
     CDS             <1..523
                     /locus_tag="VFQ61_35160"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET9959803.1"
                     /translation="AWGWVYGFPMFERDETTGAVEFGHNPFSMPAGGLEALNTKDPLE
                     IHAQQYDLFCNGLELSSGAVRNYDAEVMYRAFEIAGHTKADVDAKFGHMLQAFSLGAP
                     PHAGIAPGVERIVMLISGEPNLREVVPFPKNQQARDLMSNAPSEVLPKQLEELHIRVV
                     PPPPPPPKSPQQS"
     gene            complement(604..1959)
                     /locus_tag="VFQ61_35165"
     CDS             complement(604..1959)
                     /locus_tag="VFQ61_35165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET9959804.1"
                     /translation="MKFSLQGLLSLAWPIVLSRATQAVIGFSDALMVAPLGEDALAAA
                     TTGGLNTFLLIILPMGTAFILQSFAAQLRGRGQLDALPRYALYGLLLALGAGLLALCA
                     LPFVPWLNSKFAFTPEVQALMNSYMAVRLLSVAPATATEALGNWYGGLGNTRIGMVAS
                     VAAMLTNLIGNYALIMPRFGLPGYGVAGAAWASTAGTCVGCAVVLTAFLRGVGYERSA
                     SRGKLSLGELRRVLRFGLPNGVNWFLEFAAFTLFINVVVGHLGTTALAAFNVVMSMNS
                     VSFMPAFGVTSAGAIFVGEAIGRRAHHEVGAVVKTTGLVAASWMGAVGILYWSFPRQL
                     FAIFQPDAHGSQLAEAGTLMLGLAAFWQLFDALSMTVSEALRAAGDTAWCLAARLVLA
                     WVLFTPIAWYIGLYRNGGITGIMFSLIAYMAALTLTMVGRFASGRWKQIDLVSVEPKL
                     V"
     gene            complement(2015..3577)
                     /locus_tag="VFQ61_35170"
     CDS             complement(2015..3577)
                     /locus_tag="VFQ61_35170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959805.1"
                     /translation="MLSPAPRKSRELQLAIAILSAHFSLGYCRVALAADPALPAATAA
                     AAPAPSTTPQTSATTSAAGTTVADATVADATNAPSGAGASVEAKAALSTPSAAPLTMD
                     APSASPFRYQPSIPEPESGVARLPAEAYPNTPIRGIVGGSLWLTFHGLQWPYSPFVTK
                     QPVTQLGLSGYVWLDTGYRTLDSSAPNGVDSKTWFQQGRFVLRATPTYSKADYFVQGQ
                     AELVGNKDPSQPYPGAISVDDVWLKAGRWNAYDVQVGRFEAWELYHLGMGMDQYTLER
                     RGAYSEYNSRPAELYGVSFAYYRPSSLGNLAVHLYPTQYLRFELLGQAGNSLPQFGST
                     PPSGPESRQNMLAGRAAGVLDLGILKVKAGGEYLKSWPAVTTTQQDATAKGFGGSVQV
                     VLDPHVEFGGNLAYNIIDARDATGNVDQAGSTDTYSFGGFANGRVIENLLVGVGVNYT
                     TTADIKYDATNQVGMFSHLQGFGAVQYLLLDQLFLKAVVSYASANQAPGLPLAEYTDK
                     MLGGRVRAMYLF"
     gene            complement(3674..3880)
                     /locus_tag="VFQ61_35175"
     CDS             complement(3674..3880)
                     /locus_tag="VFQ61_35175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959806.1"
                     /translation="MTFRGSKLELVRDGSAGYGLAFQFEPNTGLGDHFAQHNRSEALR
                     PEWPFAPRQTAQIWLRASTREVLE"
     gene            4084..4971
                     /locus_tag="VFQ61_35180"
     CDS             4084..4971
                     /locus_tag="VFQ61_35180"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET9959807.1"
                     /translation="MTPAWTPVATPSAASHPEQPRPCGVVSLLTDFGVREPFVGIMKA
                     VMLRQVAGVQVIDLCHGVPPGSVEIAGFFLERSFGWFPEGTVHCCVVDPGVGSERNAI
                     AVRARGHFFVAPDNGLLTPLLEAKGEPLVGAGAEFRRIDATRLGLALRSRTFHGRDVF
                     APVAAALASGQLAFSEVGESLAHLVPAPPTLPVARRDGACARGRVLAVDHYGNLITDL
                     DGAWLEPRARVEVAGRSLRVVGTYSEAEPGEVVALVNSFEMVEIALRDGSAERALNVT
                     TGAPTRILYGASGTRESEK"
BASE COUNT          943 a         1524 c         1676 g          891 t
ORIGIN      
        1 ggcttggggc tgggtctacg gtttcccgat gttcgagcgt gacgagacga cgggcgcggt
       61 ggagttcggg cacaacccgt tctcgatgcc cgccggtggt ctcgaagctc taaataccaa
      121 ggatccgctc gagatccacg ctcagcagta cgacctgttt tgtaacgggc tcgagctgag
      181 cagcggtgcc gtccgtaact acgacgccga ggtgatgtac cgagcgttcg aaatcgccgg
      241 tcataccaag gcggacgtcg acgcgaagtt cggacacatg ctgcaggctt tcagcttggg
      301 cgcgccgccc cacgccggta ttgcgccggg cgtggagcgc atcgtgatgc tgatctcggg
      361 cgagccgaac ttgcgtgagg tggtgccctt cccgaagaac cagcaggccc gcgacttgat
      421 gtcgaatgcg ccgagcgagg tgctgcccaa gcagctcgag gagctccaca tccgagtggt
      481 tccgccgccc ccgcctccgc ccaaaagtcc gcagcagagc tgagaattac ctccggatcc
      541 tgggctcccg tggagtatct cccgcgggag cccagcactg ttccgagagc gcccggctga
      601 gcctcacacg agcttgggct cgacgctgac caggtcgatt tgcttccaac gccccgacgc
      661 aaaacgcccg accatcgtca gcgtcaacgc ggccatgtac gcgatcagcg aaaacatgat
      721 gccggtgatg ccgccgttcc ggtacagccc gatgtaccag gcgattggcg tgaagagcac
      781 ccacgcgagc accaagcgcg ccgccagaca ccaagcggtg tctccggcgg cgcggagcgc
      841 ctccgaaact gtcatcgaga gcgcatcgaa cagctgccaa aatgcggcta ggccgagcat
      901 cagcgtccct gcttcggcga gttggctgcc atgcgcatcg ggttggaaga tggcgaagag
      961 ttgccggggg aatgaccaat agaggatgcc gacggcaccc atccagctcg cggccacgag
     1021 gccagtggtc ttcacgactg ctccgacctc gtggtgcgcg cgacgtccga tggcctcccc
     1081 cacgaagatg gcgcccgcgg aagtcacccc gaacgcgggc atgaacgaaa cggagttcat
     1141 gctcatcacc acgttgaacg ccgcaagtgc cgtggtcccc aagtgcccga cgaccacgtt
     1201 gatgaacaac gtgaaggctg caaactcgag gaaccagttc acgccgttgg gcaacccaaa
     1261 gcgcaatact cgccggagct cgccgaggct cagcttccct cgcgaagccg agcgctcgta
     1321 gcccactccc cgcaggaaag cagtcagcac cacggcgcaa cccacgcagg ttccggccgt
     1381 gctcgcccaa gcggcgccgg ctacgccgta acccggcaga ccgaagcggg gcatgatcag
     1441 cgcgtagttt ccgatcaggt tcgtcagcat ggccgcgacg gacgccacca ttccaatgcg
     1501 tgtgttgccg aggccgccgt accaattgcc cagcgcttcg gtcgcagtgg cgggcgccac
     1561 cgagagcaag cgcaccgcca tgtacgagtt catgagcgct tgaacctcgg gcgtgaacgc
     1621 gaatttacta ttgagccacg gcacgaatgg caaagcgcag agcgccagta gaccagcgcc
     1681 caaggccaga agcaggccat acagcgcata acgaggcaaa gcatctagct gcccgcggcc
     1741 gcggagctga gccgcgaagc tctgcagaat gaaggccgtg cccatcggca agatgatgag
     1801 caagaacgta ttgaggcctc cggtcgtggc cgcggccaaa gcgtcttcac cgagcggcgc
     1861 gaccatcagc gcgtcactga agccaatgac cgcttgggtc gcgcgcgaca gcacgatcgg
     1921 ccaagccaac gagagtaacc cctgcaatga gaacttcatg acccgggctg aggtgtaccc
     1981 tcgaaacgag ggctcggcct caaaatccgc gcgcctagaa caggtacatc gcgcgcactc
     2041 gcccgccgag catcttgtcg gtgtactcgg ctaggggcag ccctggggct tggttggccg
     2101 aggcgtaact cacgacggcc ttgagaaaca gctgatccag cagcaagtac tgtacggccc
     2161 cgaagccttg caggtgtgag aacatcccaa cctgattggt ggcgtcgtac ttgatgtccg
     2221 cggtggtcgt gtagttcacg cctaccccca ccagcaagtt ctcgatcacc cggccgttcg
     2281 cgaagccccc gaagctatag gtatcggtgc tgccggcttg gtccacgttg ccggtcgcat
     2341 cacgcgcgtc gatgatgttg taagcgagat tgccgccgaa ttcgacgtga gggtcgagca
     2401 ccacctgcac actgccgcca aagcctttgg cggtcgcatc ttgttgcgtc gtcgtgactg
     2461 ccggccacga tttgaggtac tcgccgccgg ccttcacctt gagaattccg aggtcgagca
     2521 cacccgcggc gcgccccgcc agcatgttct gtcgtgactc tggcccgctc ggtggcgtgc
     2581 tgccgaattg cggtagagaa ttgccggctt ggccgaggag ctcgaatcgc agatattgag
     2641 tcggataaag atgaaccgcc aagttgccga gtgagctcgg tcggtagtag gcgaagctga
     2701 ctccatatag ctccgcgggc cttgagttgt actcgctata ggcccctcgg cgctcgagcg
     2761 tatactggtc catccccata cccagatgat agagctccca ggcctcgaag cgcccaactt
     2821 gcacgtcata ggcattccaa cgtcccgcct tgagccagac gtcgtcgacg ctaatcgcgc
     2881 cggggtaggg ttgactcggg tctttattgc cgaccaactc ggcctgacct tgcacgaagt
     2941 agtcggcttt cgaatacgtc ggcgtggctc gcaatacgaa acgaccttgt tgaaaccaag
     3001 tcttcgagtc tacgccgttt ggcgcggagc tatccagagt acgataaccc gtgtctagcc
     3061 agacgtagcc agagaggccg agttgcgtaa cgggttgctt ggtgacgaag ggagagtagg
     3121 gccattgtag gccgtggaac gtgagccaca acgagccgcc cacgatgccg cgaattggtg
     3181 tgttcggata agcctcggcg ggcaagcgag caacaccaga ctctggctct ggaatgctag
     3241 gttggtagcg aaatggggac gccgaggggg catccatcgt gagtggtgca gcgctaggcg
     3301 tcgacagcgc cgctttggcc tctacgctgg ccccagctcc gcttggcgcg ttggtggcat
     3361 cggcgacggt ggcatcggca acggtggtgc ccgcggcgga cgtggtcgcg gatgtttgcg
     3421 gcgtggtaga cggcgcggga gctgcggctg cggttgcagc gggcagcgcg ggatcggctg
     3481 ccaacgcgac ccggcaatag ccaagactga agtgagccga gaggatggcg atcgccagtt
     3541 ggagctctcg agacttgcga ggggcggggc taagcacggc cgcggtactc tacaggaatc
     3601 gcaatctgac gagatccaaa cgcaggtcac gaccgtgttc ttgggttgcc tggcggctgg
     3661 gcggcgtgca gcgctattcc aatacttcac gggtactcgc gcgcagccag atctgcgcgg
     3721 tttgacgggg ggcaaatggc cactcgggcc gcagggcttc cgagcggtta tgctgggcga
     3781 aatggtctcc gagtccggtg ttcggttcga attgaaacgc aagtccgtag cccgcggagc
     3841 cgtcgcgtac gagctcgagc ttcgaaccgc gaaaggtcat tgggtgggaa gcgcgagttg
     3901 cgagcaggcg ggtcagcagt ttgtcgtcca cttcgagtcg gagggcccgc cggagtggtg
     3961 cgtaacaatc ttggcggcca ctctccgtgg agttgcacga gatgccgcgg ccaagggcga
     4021 ctaccccagg cgcattgcga ggtggcgacc cgaacccggc gcgtccagcg gggaggatgg
     4081 tgcatgaccc cggcttggac gccggtagct acgccctcag cagcctcgca cccggagcag
     4141 ccgcggccat gcggcgtcgt cagcttgctc accgatttcg gtgtgcgtga gccgttcgtg
     4201 ggcatcatga aggcggtgat gctgcgccaa gtcgcagggg tgcaggtcat cgacttgtgt
     4261 cacggggttc cacccggcag tgtcgagatt gcaggcttct tcctggagcg cagcttcggc
     4321 tggtttccgg agggtacggt gcattgttgt gtggtcgatc cgggcgtggg cagcgagcgc
     4381 aatgcaattg cagttcgcgc acgcgggcac ttcttcgtcg cgccggacaa cgggttgctg
     4441 acgccgttgc tcgaagccaa aggcgaaccc ttggttggag ccggcgcgga gttccgtcgt
     4501 atcgatgcga cgcgcttggg tctcgccctg cgcagccgaa cttttcacgg tcgagatgtg
     4561 tttgctccag tcgcggccgc tctggcatcc gggcaactcg ctttcagcga agtcggcgag
     4621 agcctcgcgc atctcgtgcc tgcacccccg acgcttccgg ttgcgaggcg cgacggtgct
     4681 tgtgcgcgcg ggcgagtgct ggcggtcgac cactacggta acttgattac cgatctcgat
     4741 ggggcttggc tggagccgcg ggcgcgcgtg gaagtcgccg ggcggagctt gcgcgtggtg
     4801 gggacgtact ctgaggcgga gcccggagaa gtcgttgcac tcgtgaattc gttcgagatg
     4861 gtggagattg ctttacgtga tgggagtgcg gagcgggcgt tgaacgttac gacgggcgca
     4921 ccgacacgga ttctttacgg tgcgtccggt acgagagaga gcgagaaatg aggggcggac
     4981 aggtgcagtg gagctcgggc attggactgc gcgcggcccg ggccgcgggc atag
//
LOCUS       DASTKU010000990        11273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_862745, whole genome shotgun sequence.
ACCESSION   DASTKU010000990 DASTKU010000000
VERSION     DASTKU010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11273
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862745"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFQ61_35185"
     CDS             <1..496
                     /locus_tag="VFQ61_35185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959808.1"
                     /translation="GPSDKQLNHLKKESELGVRFRVIVPKGGNGSGARVVLRARCDKH
                     PTPNNSTSEGAEERGRIVVGSAANLQRYDREGKAVPERRIPELDSRRALKLELSCDGE
                     PHEVFGTLPKSLAANPGSSVEYVLFASRGVNVEGVAIVPLADELPPPPPEPWRPSSSA
                     AKTE"
     gene            547..1851
                     /locus_tag="VFQ61_35190"
     CDS             547..1851
                     /locus_tag="VFQ61_35190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959809.1"
                     /translation="MADPKQPSEREIEADEAASNAASSRESEPTDSNESDSNESEPAE
                     TAEGELAGSADRPTPMNRAEARKAARSKGGSEDIRDRNKRVREEAAARRRSRRDAEER
                     VVQARNLDASEIVDDALARTTQAAGNFLKRHINTVQWLIVLGVVSGIGYQIWSYRHHR
                     TTARTADELAKALVQENARVGAEPAENQESAYYEAPDPRPSFTDDAARLKAAGEQYSA
                     VKSSTSGKISTLAALGLAGVLYDQGKFKAARDEYTAVKGSELAKLDNDVRGRSLEGIG
                     LTLEAEQQIDAALAAFRELENTDVPGLSALGQYHQARLLLKKGEREPAKKLLTQAQEK
                     LKKLSEAEQAGPDQLSARGGFLEHQVRELLITVDPSAAPKAPAGGLSPEQIRRLQSQL
                     GGGDGKMDPAKLKELLEKLSKPATPGSPPAGDSTPAPAGSAP"
     gene            1863..3224
                     /locus_tag="VFQ61_35195"
     CDS             1863..3224
                     /locus_tag="VFQ61_35195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9959810.1"
                     /translation="MKRSVGPRFDLLGSQWFGGLSLCVALGCALSGSGCGAVFGSGAN
                     PELPVWMQRPSGSLEVVYSRGALAESRRAGEPYERGQPEIDVSGRRVFVGSSDRGLYA
                     LRAEDGTQLWRFETLGFVQCQPLYDRKEDALYFGSNDGALYRVDARDGHLRWRFMSNA
                     EVSRRPVLSDGILYATNANDTVLAIDPNTGKLLWHQHRTPAMGMEIANYAGVTVYRGK
                     VYTGFSDGTVLAFDAKTGEERWQPVDLSAEAEQTLGELPQQLDVDTTPVPDQIDAGAV
                     VYVASYAGGLFALDAETGAQVWSNPGVAGATDLFLWDEPGRPATRLPDGEPAAQTETA
                     PRKILIASSGTTGLWGIDPESGKELWRRSVPRGGVSAPVPFQGALLVTASQLGVFLIS
                     PLGGELIDGLHVAEGVSMTPAVHGNRAFVMTNGGRFLSLHVAAPKPGSRPPAAWEFKS
                     PEG"
     gene            complement(3523..4290)
                     /locus_tag="VFQ61_35200"
     CDS             complement(3523..4290)
                     /locus_tag="VFQ61_35200"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HET9959811.1"
                     /translation="MDLRVIGCHGGETPRHRTSAFLVDDQVAIDAGSLTAGMELDEQY
                     RLGACLISHAHMDHVRDLAMLADNRCQAGCEPLVVAANKATIAALKKHFFNDVLWPDF
                     SKIPSAGKPTIRFLELVPERRTAIGNYFVRAVKVNHTIDTSGFLIEGPEASLAYSGDT
                     GPTDRLWELLNEARNLKALLLEVSFPNAEQKFATLSGHHTPRTLHEDLKKLAHPKDLP
                     TLLYHMKPAHQSAIEKECAKLRGVNLTVLNTGDQFVL"
     gene            4644..4714
                     /locus_tag="VFQ61_35205"
     tRNA            4644..4714
                     /locus_tag="VFQ61_35205"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4676..4678,aa:Gln,seq:ctg)
     gene            4810..4884
                     /locus_tag="VFQ61_35210"
     tRNA            4810..4884
                     /locus_tag="VFQ61_35210"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4843..4845,aa:Glu,seq:ctc)
     gene            complement(5435..5917)
                     /locus_tag="VFQ61_35215"
     CDS             complement(5435..5917)
                     /locus_tag="VFQ61_35215"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET9959812.1"
                     /translation="MKIRELMTRNVACIRCEDCLSSAAQLMWDCDCGAVPVLDSTGCV
                     VGMITDRDICMAAWSKDRPLSSIAVSDAMSKTLYHCTPEDTVSFVENLMRSKQIRRIP
                     VLAKDGSLAGIVSLADIVMHSQNVGTRAAAHELAPHEVTTTLANICQPRPSSLGNARA
                     "
     gene            complement(6010..7500)
                     /locus_tag="VFQ61_35220"
     CDS             complement(6010..7500)
                     /locus_tag="VFQ61_35220"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959813.1"
                     /translation="MDPSEPCLLEEPHHLVRGLHEASCDDELQERDRFIRELKSQGGS
                     GQPLHGLFDGKLELGIARAPGRLDVMGGIADYSGSLVLEWPTREAAWAAVQVHPERSL
                     RVASRDPAAPSHWRQATLPIDPLLTLEPRAVGTWLRQCGAGWAGYVVGALALLPEVRR
                     RSFQGGLRVAICSNVPEGKGVSSSAAIEVAAYFALAKVLGWAVDAREAALACQRVENQ
                     IVGAPCGAMDQLTSACGLKGRLLEVLCQPATVLNALTLPEDLELFGIDSGQRHAIRDP
                     AYGRVRAAAFMGLRILTSFARSGAGQEEEPFGGYLANVSPSQLDSGMLERLPEFMTGA
                     EFSSRYGALEDTVSVIEASGTYPVRTATAHPIREHHRVRLFAELLRGLSGADRHDGLR
                     ARELLGELMYQSHASYSACGLGAPGTDRLVALAREVGAGGGVFGAKISGGGSGGTVVV
                     LVARSARAAIDRIAKQYASETGVAAQVFSGTSAGAGEFGATSVMLD"
     gene            complement(7506..8534)
                     /gene="galT"
                     /locus_tag="VFQ61_35225"
     CDS             complement(7506..8534)
                     /gene="galT"
                     /locus_tag="VFQ61_35225"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET9959814.1"
                     /translation="MVWEERWHPLRREWVTVAAHRQNRPWSGNTVGESARRPPAYDPS
                     CFLCPGNARVSGRRNPVYSNVFVFDNDHPCVGPDAPLVLPAPAGIYRVQPATGLARVV
                     CYSPRHDLTLAELELVEVEALLREWQQQYRELGARPEVKNVLIFENKGEVVGVSNPHP
                     HCQIYATNFVFRTIEVEIEASRAHFEQTGRLLFQDILSAEREDGRRLLAENATAIAFV
                     PYFARYAYEVYVAPKRAVQSIADLDLRELSDLARVLLDVVVAFDNLWKLSFPYVMTLH
                     QAPTDGGDYPTFQFHIVFQPPLRKPNLLKYLAGPEIGGGCFLSDTCPEEKAQDLRNAL
                     GPHYGAAG"
     gene            8942..9628
                     /locus_tag="VFQ61_35230"
     CDS             8942..9628
                     /locus_tag="VFQ61_35230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959815.1"
                     /translation="MNALNWSRLFAFALAGSSFWIGCAGHEPGGHHGPAQCGSLECAP
                     GEHCELQDVVCKRAPCDPVAQCVPDVEGPFCGGLAGIPCPGSGRCSDDPSDDCDPANG
                     GADCGGVCTCLQTALCIQGLVFDSSPEVCQCVPDPNQDICSRVRCAAGTHCESGVGCV
                     PDEPNPCAAVLCRTGTICEVHEGEAYCVSDGTQECGKTTCGAGSVCCNGSCGICTRPG
                     MACIQIACTD"
     gene            complement(10433..>11273)
                     /locus_tag="VFQ61_35235"
     CDS             complement(10433..>11273)
                     /locus_tag="VFQ61_35235"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9959816.1"
                     /translation="ILGPWGEKQALFPPLDVPREESDEPDYPSPEQNEILLHPRVQQF
                     GSAVYVRLNVSRMKAISVASEERPHMNVDGYGLPSVLAWMKGAEEERLAEITANARKI
                     VPGIRRIKTLRKFVRSQRMDSLDVAGQKVWRPVNEERLGDRFEVEFDNGASVPADLLS
                     EGTVVALGLLATLASKNRPRLILADDIDRGLHVEAQSKLVDVMRELLATDPDLQIVCT
                     THSPYLLDRFKPDEVRVLSLDSSRHTHARALTDHPDFEKWKFGNQTGELWAALGDAWV
                     ASE"
BASE COUNT         2181 a         3509 c         3507 g         2076 t
ORIGIN      
        1 ggggcctagt gacaagcagc tcaaccatct caagaaagag agcgagctcg gggttcgctt
       61 tcgagtgatc gtgcccaagg gcggtaacgg tagcggcgct cgagtcgtgc tgcgcgcccg
      121 ctgtgataag catccgaccc cgaacaactc gaccagcgag ggcgcggagg agcgcggacg
      181 aatcgtggtg gggtcggccg cgaacctcca acgctacgac cgcgagggaa aggcagtccc
      241 ggagcgcaga attcccgagc tcgattcgcg gcgcgcgctg aagctcgagc tcagctgtga
      301 cggtgaaccg cacgaggtgt tcggtacgct gccgaagagc ttggcggcca accctgggtc
      361 gagcgtggaa tatgtgctgt tcgcgagcag gggagtcaac gtcgagggag tggccatcgt
      421 accgctcgcc gatgaattgc cccctccacc cccagaaccc tggcggccgt cgagttccgc
      481 cgccaaaacc gagtaaattc tcgcgcagac tacggattgc gggggggtgt ggtatagacg
      541 cgcgccgtgg ccgacccgaa acagccctcc gaacgcgaaa tcgaggccga cgaagctgct
      601 tccaacgctg cttcttcgcg cgaatccgag cccacagatt cgaacgaatc cgattcgaac
      661 gaatcagaac ccgccgagac cgctgaaggc gagctcgcgg gctctgccga tcggcccacg
      721 ccgatgaatc gggccgaagc ccgcaaagcc gcgcgcagca aaggcgggag cgaggatatt
      781 cgagaccgca acaagcgagt gcgggaagag gccgccgcgc ggcgtcgctc tcgaagggac
      841 gcggaggaac gcgtggtgca agcgcggaac ttagacgcga gcgaaatcgt cgacgacgcg
      901 ttggctcgga cgacgcaagc ggcaggcaat tttctgaagc ggcacatcaa taccgtgcag
      961 tggttgatcg tgctcggagt ggtatccggc atcggctatc agatttggag ctaccggcac
     1021 caccggacca cggctcgcac ggcggacgag ctcgccaaag ccctggttca ggagaacgcc
     1081 cgcgtgggtg ccgagcccgc ggaaaatcaa gagtcggcct actacgaagc gcccgatcca
     1141 cgccccagct tcactgacga cgctgcgcgc ctgaaagccg cgggtgagca gtacagcgcc
     1201 gtgaagagct ctacatccgg caagatctcg acgctcgcag ccctgggatt ggccggcgtt
     1261 ttgtatgacc aaggcaaatt caaggcggcc cgtgacgagt acacggccgt gaaggggtcc
     1321 gagctggcca agctggacaa tgacgtccgc ggccgttcgc tcgaaggcat cgggctcacg
     1381 ctagaagccg aacagcagat cgatgccgcg ctcgcggcct ttcgtgagct ggagaacacg
     1441 gatgtgccgg gtctgagcgc gctcgggcag taccatcagg ctcggttgct gctgaagaag
     1501 ggcgagcgag aaccggcgaa gaagctgctg acgcaagctc aggagaagct caaaaagctg
     1561 agcgaggccg agcaagccgg acccgatcaa ttgagcgctc gtggcggctt cctcgagcat
     1621 caagtccgtg aactactcat cacggtggac cccagcgccg cgcccaaagc tcctgcaggt
     1681 ggcttgagtc ccgaacagat tcgcagactg caaagccagt tgggcggcgg ggacggtaag
     1741 atggatcccg ccaaactgaa ggagctgctc gagaaactct cgaagccagc cacacccggg
     1801 tcacctcccg cgggagactc cacgccggca cccgcgggga gtgcgccttg agtcgacaat
     1861 ccatgaaacg ctcggtaggc ccgcgcttcg acttactggg ttcgcaatgg tttggtggcc
     1921 tgagcctctg cgtggcgctc ggctgtgcgc tgtcgggcag cggttgcggc gctgtgtttg
     1981 ggagcggcgc gaatcccgaa ctgcccgttt ggatgcagcg gccaagcggc agcctggagg
     2041 tggtgtacag ccgcggcgcg ctggccgagt cgcgacgagc gggggaaccg tacgagcgcg
     2101 ggcagcccga aatcgacgtc tcgggaagga gagtctttgt cggttcgagc gatcgcggct
     2161 tgtatgcgct gcgggccgaa gacggcaccc agctctggcg cttcgagacg ctgggcttcg
     2221 tgcagtgcca gccgctctac gaccgaaaag aggatgccct gtacttcggc tcgaatgacg
     2281 gagccctgta ccgcgtagac gcgcgcgatg ggcacctgcg ttggcgcttc atgtcgaacg
     2341 ccgaggtctc ccgtaggccc gtgctgagcg atggcattct ctacgcgacc aacgccaacg
     2401 acacggtgct cgcgattgat ccaaacaccg gcaagcttct ttggcaccag caccgcacgc
     2461 ccgccatggg catggaaatc gccaactacg ccggcgtcac cgtctaccga ggcaaggtct
     2521 acaccggctt cagcgacggc actgttttgg cgttcgacgc caagaccggc gaagagcgct
     2581 ggcaacctgt agacctgtcc gcggaggccg agcaaacgtt gggggaactc ccgcagcagc
     2641 tcgacgtcga taccactccg gttcccgatc aaatcgacgc gggcgcggtg gtgtacgtcg
     2701 caagctatgc gggagggctg ttcgcgctcg acgccgaaac cggagcccag gtgtggagca
     2761 atcccggtgt ggctggcgcc acggatttgt tcctctggga cgagccagga cgcccagcaa
     2821 caaggctgcc ggacggagaa cccgccgccc aaacggaaac cgcgccgcgc aaaatcttga
     2881 tcgcgtcatc gggcaccact ggcctgtggg gaatcgatcc cgaaagcgga aaagagctgt
     2941 ggcggcgcag cgtgccgcgt ggcggcgtgt ccgcgccggt tccgttccaa ggcgcgctgt
     3001 tggtgaccgc cagtcagctc ggcgtcttcc taatttcacc gctcggtggc gaactgatcg
     3061 atgggctgca tgttgccgag ggcgtatcga tgacgcccgc ggtacacggc aatcgcgcct
     3121 tcgtcatgac gaatggtggc cgcttcttgt cgctccacgt ggccgcgccg aagccaggtt
     3181 cacggccgcc ggccgcctgg gaattcaaat cgcccgaagg ctgacccggc agtcgggcaa
     3241 tcacgtcgca tgtccgggtg cgttcggagt ttgaccgcga actccgaacg ccggcggcac
     3301 agatcgacgt cgctcggccg cggacggcga atgacggcgg gcggagctct atttacggat
     3361 ccgggtcggt cccgaatggg ccgattgagg ctccaaacct ccctaaagcc gctcgtcgga
     3421 agttcgcata agatacatta tgtcaactga tcgcccacgt aagcgtgagg ggctccgtaa
     3481 gtccgccagg tcctgttcga gcttgccccc ggtctgctgc ttctaaagaa cgaactggtc
     3541 cccggtgttc aggaccgtga ggttcacgcc gcgcagcttg gcgcactcct tctcgatggc
     3601 ggactggtgc gcgggcttca tgtgatagag cagcgtcgga agatccttgg ggtgcgccag
     3661 cttcttcaga tcttcatgga gcgtgcgcgg cgtatgatgt ccgctcagcg tcgcgaattt
     3721 ctgctcagcg ttcggaaagc ttacttccag cagcagcgcc ttcaggtttc gggcctcgtt
     3781 cagcagctcc cagagtcggt ccgtcgggcc cgtgtccccg ctataggcca aggaggcctc
     3841 gggcccctcg atcaggaagc ccgaggtatc gatggtgtgg ttcaccttta ccgcgcgcac
     3901 gaagtagttt ccgatggcag tgcggcgctc gggaaccagc tccagaaaac ggatggttgg
     3961 cttacctgcc gacgggatct tgctgaagtc tggccagagc acgtcgttga aaaagtgctt
     4021 cttgagcgcg gcgattgtcg ccttgttcgc cgcaaccacc agcggctcgc accccgcttg
     4081 gcaccggtta tctgcgagca tcgctaggtc ccgcacgtgg tccatgtgcg cgtggctaat
     4141 taagcaagcc cccagacggt attgctcatc cagctccatg ccggccgtca gcgatccggc
     4201 gtcgatggca acctgatcgt ccacgaggaa agcgctcgtg cgatgccgag gggtctcgcc
     4261 gccatggcag ccgatcacgc gcaggtccat ctggatgatt cttatagctg cccgctggcg
     4321 cagttcgcga acggctttcc tgcaaaagaa gcaatcctcg tccccaggtg cccccgaaga
     4381 cggctctggc tcaaagatta tgtcaactag aactcgcttc gaggtgtgtc attccggcca
     4441 acgggtccga gattgcttca gaaaatctta ccgggacgcg ttgtgaaccc ttgcgcagtt
     4501 cggggatcag agatagtaac gccacccatc gcggtgccgg gcaggccttc tggccagcgg
     4561 cgctgtgttg ccttccttag tattgattcg ccttgtaggg cgctcgtcgg caagtctatc
     4621 ccctctttga agggaccctc gattggggga tcgtctaacg gcaggacgca agattctggc
     4681 tcttgctatc taggttcgaa tcctagtccc ccagctcact ttcttagcct tcgtaactct
     4741 taaagcttag tagtcagtca ccagtcgagt agtgaggcat cggccgtctc ggtcgacgca
     4801 gttcggttag gccccatcgt ctagcggtta ggacatcggc ctctcacgcc ggtaacgcgg
     4861 gttcaattcc cgctggggtc gccaaagtta cgtcgagctg cgcgtaagcg cggccgtata
     4921 cgttgcgcac agcgtgtaga agggctcggt tctattccga gccctgtcac gcctaacttc
     4981 aagcaacggg cttagcttac gttcgcgcga tccgaacgag tcccgcaggt caccgctgag
     5041 cctttggcgg cgcggcgttc cgtgcagagc tctagagctg cggcgggcgg ggctcgagcg
     5101 gtgcagcgtt ccgtgccggt agctcttccg aagccctgtg cgctcggtct agccgaacga
     5161 gctcctctgc atagctcatt cggaatgatt gctgctcttc cggggtgagg ccggagggaa
     5221 ctggggcttc tcgttcggcc tcggaccacg ccagcggtcg cgctggtacc gagcggcttc
     5281 gggcggcgtt ctttgccagc agttggatgt cagccatgac tagaaaactc ctacgagcgt
     5341 agttgcagac gatcctggtg agagctgcgc cctaggaagc catgtgcatt ccgtgcacat
     5401 ggggcgttcg cgagaccggc tcgcctgcac agatttacgc gcgagcgttc ccgagtgagc
     5461 tcgggcgcgg ttggcagatg ttggcgagtg tggtggtcac ctcgtgggga gccagctcgt
     5521 gtgccgcagc ccgagttcca acgttctgtg agtgcatgac gatgtccgcg agcgaaacga
     5581 tacccgccaa acttccgtct ttcgcaagca cagggatgcg ccggatctgc ttggaccgca
     5641 tcagattctc gacgaacgag acagtgtcct ccggggtgca gtgatagagc gtcttcgaca
     5701 tagcgtcgga gaccgcgatc gagctaagcg gccgatcctt gctccatgcg gccatgcaga
     5761 tgtcgcggtc cgtgatcatg cccactacgc agcccgtgct atcgagcacc ggaacggcgc
     5821 cacagtcaca atcccacatc agctgtgctg cgctgctcag gcagtcctca caacggatgc
     5881 aggcgacgtt gcgtgtcata agttcccgaa ttttcatgcg acgctccttc ggctggtttc
     5941 gtcagccgag cttctcgggt ttgcaaaatg cgcaccgccg tgtggcggga catgcctgcc
     6001 tcaagtccgc taatcgagca ttacggaggt cgctccaaac tctcccgcgc cggccgacgt
     6061 tccactgaag acctgagccg ctacccccgt ctcgctcgca tactgttttg caattcgatc
     6121 gatagccgct cgcgccgagc gcgcgaccaa aacgacaacc gttcccccac tccctccccc
     6181 agaaatcttc gcgccgaaca caccaccacc ggcgcccacc tcacgcgcga gagccacgag
     6241 ccgatcggtg ccgggggcac ccaggccaca agcggaatag ctcgcgtgtg actgatacat
     6301 gagttctccc agcagctcac gagctcgcag cccgtcgtgc cggtcggcgc ccgagagtcc
     6361 acgcagaagc tcggcgaaca ggcgcacccg gtggtgttcg cgaatcgggt gcgccgtggc
     6421 ggtgcgcacc ggatacgtgc cactggcctc gatgacactc accgtatcct cgagcgcacc
     6481 atagcgggag gagaactccg ctccggtcat gaactcgggc agacgttcca gcatgccgga
     6541 atcgagctgc gacgggctaa cgttggcgag atagccgcca aacggctcct cctcctggcc
     6601 agcgcccgag cgcgcgaagc tcgtcaagat gcgaagcccc atgaatgccg ccgcacgaac
     6661 gcgcccgtac gcgggatcgc ggatggcgtg gcgctggccg gagtcgatac cgaacaactc
     6721 caagtcttcg ggcaacgtga gagcattcag taccgttgcg ggctgacaca ggacctcgag
     6781 cagccgtccc ttcaatccac acgccgaagt gagttgatcc atcgctccgc acggcgcacc
     6841 tacgatttgg ttctcgacac gttggcaagc gagagcagct tcacgagcgt cgacggccca
     6901 gcccaatacc ttcgccaacg cgaaataggc cgccacctcg atggcagcgg acgaactcac
     6961 gcccttgccc tccggcacgt tggagcagat cgcgacgcga agaccgccct gaaagcttcg
     7021 tctacgaacc tccggcagca gcgcgagtgc accgacgacg tagcccgccc aacctgcccc
     7081 gcattgcctt agccatgttc cgactgcgcg gggctcgaga gtcagcagag gatcgattgg
     7141 cagagtcgct tggcgccagt ggctcggtgc tgccggatcg cgacttgcaa cgcgcaagct
     7201 cctttctgga tgcacctgaa cggcggccca cgccgcttct cgagtcggcc attcgagcac
     7261 caaagagcca gaatagtcgg caatgccgcc catcacgtcc aaacgcccgg gagctcgagc
     7321 aatccccaac tccaacttgc catcgaacaa cccatgcagc ggctgacccg agccgccttg
     7381 gctcttgagt tcgcgaatga accgatctcg ttcctggagc tcatcgtcac aggacgcttc
     7441 gtggaggccg cggactagat gatgaggctc ctccaggaga caaggctcgc tgggatccat
     7501 gcgcttcacc ctgcagctcc gtagtgggga ccgagggcgt tcctcaagtc ctgcgctttc
     7561 tcttcggggc aagtgtcgct gagaaaacag ccgccaccga tctccggtcc ggcgagatac
     7621 ttgagcaggt tgggcttacg cagcggcggc tgaaagacga tgtggaattg gaaagttggg
     7681 tagtccccgc cgtcggtggg cgcctggtgc aaggtcatca cgtacggaaa agagagcttc
     7741 caaaggttgt cgaaagccac gaccacatcc agcagtacgc gcgctagatc actcagctcg
     7801 cgcaagtcta ggtcggcaat actctgcacc gcgcgcttgg gtgccacgta tacctcgtaa
     7861 gcgtaacggg cgaagtacgg cacgaatgcg attgcggtcg cattctcggc gagcagtcgg
     7921 cgcccgtctt cgcgctctgc gctcaggatg tcttggaata ggaggcgccc ggtctgctcg
     7981 aagtgggctc gcgaggcttc gatctccact tcgatggtgc ggaatacgaa gttggttgca
     8041 tagatctggc agtgggggtg cggattggag acccccacca cttccccctt gttctcgaag
     8101 atgaggacgt tcttcacttc tggccgagcg ccgagctccc gatactgctg ctgccattcc
     8161 cgcaacaggg cttcgacttc gacgagttcg agctcagcca aagtcaggtc gtgacgcggg
     8221 gagtagcaaa ctacgcgagc caagccggtg gctggctgca ctcggtagat gcctgcgggt
     8281 gcgggcaaca ccaacggtgc atcgggtccc acgcaggggt gatcgttgtc gaacacgaac
     8341 acgttcgagt agaccggatt cctgcgtccg ctcacgcgcg cattgccagg acataaaaaa
     8401 cagctgggat cgtacgccgg agggcgccgc gcgctctcgc cgacggtgtt gcccgaccag
     8461 ggccgattct ggcgatgtgc agccaccgtg acccactcac gccgaagcgg atgccagcgt
     8521 tcttcccaga ccatcgagtt cctccgaaaa agttcctcgc ctcaatgcga gtcgagcgac
     8581 gcagccagca cgatagtgtg atcacgcact gaaatggaag taccggtcga agtaagctgt
     8641 gtggaaggtg cctgtgtgca cagtgtggac gtgggcagca tcggtgcaaa cacggtcgaa
     8701 gcagtgcggc aagttcgcga tgcaagcact tcagtgcagg tttctcacga gcacggcatc
     8761 gcgaactgca cgtgcagagc gataacgcgc ttgcacgtcc gcgcccttcg cggtgcttcg
     8821 tgcggccaga gcgcaccttg ttacgtttgt ttgcggttcg cgaatgctgc tgctcgaaca
     8881 gcgagagcgc gtatactggg cgcgagggta tcgcaagctt cgcaccctct ggaggtggct
     8941 gatgaatgca ctgaactggt cacgcttatt cgcgttcgct ctagcaggtt cctcgttctg
     9001 gatcggatgt gctgggcatg aaccgggtgg gcaccacggg cccgcgcaat gtggctcgct
     9061 ggagtgcgcg ccgggagaac attgcgaact gcaggatgta gtctgcaagc gcgcgccttg
     9121 cgatccggtt gcgcagtgcg taccggacgt cgaagggcca ttctgcggcg gcctcgcggg
     9181 catcccctgc ccgggatcgg gtcgctgtag cgacgatccg agtgacgact gcgatcccgc
     9241 gaacggcggc gcggactgcg gcggagtctg tacgtgcttg cagactgcgc tctgtatcca
     9301 gggcttggtg ttcgacagct cgcccgaagt ctgtcagtgc gtgcccgatc cgaaccagga
     9361 catctgctcg cgcgtacggt gcgcggccgg cacgcactgc gagtcaggcg ttggttgcgt
     9421 ccccgacgaa cccaatccct gcgcggccgt gctgtgccgt acggggacca tctgtgaagt
     9481 ccacgagggc gaggcctact gcgtctcgga tggcacgcaa gaatgcggca aaaccacctg
     9541 tggcgcaggc tcggtctgct gcaacggaag ctgcggaatc tgcacccgcc ccggcatggc
     9601 ctgcatccaa atcgcctgca cggactagcg cgattggaca ggcgctcgca aagcccggct
     9661 tgagccgagg ctttgcgagt gcctcaacga gccccgaata gagggacgat catctgctga
     9721 acatccaata ggaaggcttt cacgccgttc tgcgcaccac aatcacctat ctctcttaag
     9781 tttgcgatgc acgacagttg ccgttgctcg tctttgccgc aaagggctct gagaaccgct
     9841 ttcgcatccg tgagtgcatc gttcgggtga gaagtcagcc gatgggattc tctcactgga
     9901 tgaaaggaaa gaatggagca caatcgatcg agcatttccc gctcgtcatc gtctgccggt
     9961 tcgaatccgg cgaccaacca tgcctctgct tcaggctgag gcgcagccac aacaaccggg
    10021 aagggccaag gcaggtgatc tctcacttgg cagatcccgt cccggcgttc acgatttcca
    10081 tctagatcac gagcaagcac gacgacgtcc ggtcgaggct ctgagtgaca gaacatcatc
    10141 agtacgtttc gccacatgct tgcttccggt ttcaggggcc tgccgccaat gtgaccatgc
    10201 cgggcgatcc tttggccgag cacgtgcgct ggccggaggt cacgcgcgtc ccgcggatcg
    10261 tacttgtacc aagactctcc ctcattccta ccccgaaaag ttcggcagaa ttcaagcacc
    10321 ccatcgagcc aatcgtgaga cgcgagcagc acctcatcga tgagctgcgt tacgacgacg
    10381 aaatgccccc gatcctctgc cgcgatcccc accagaactc tatccaccgg cgctactccg
    10441 aggccaccca ggcatcgcca agtgctgccc acagctcgcc cgtctgattc ccgaacttcc
    10501 acttctcgaa gtcgggatga tcggtcaagg cgcgtgcgtg cgtgtgtcgt gaggagtcga
    10561 gggagagcac ccgcacctca tctggtttga agcgatccaa caagtaaggg gaatgggtcg
    10621 tacagacgat ctgcagatcg ggatccgtag caagtagctc gcgcattacg tcaacgagct
    10681 tgctttgggc ttcgacgtga aggccacgat ctatatcgtc cgcgaggatc agcctgggtc
    10741 tgttcttgct cgctaaagtg gccagtagac cgagggcaac gacggttcct tcactgagaa
    10801 gatcggcggg gaccgatgca ccgttatcga actccacttc gaaacggtct cccagccgtt
    10861 cttcattgac cggccgccat actttctggc cggccacgtc cagcgaatcc attcgttgac
    10921 tacgaacgaa cttccgcagc gttttgatcc ggcggatacc cggaactatc ttgcgagcat
    10981 tagcggtgat ctcagcgagc cgttcctcct cggcaccctt catccaggca agcacagacg
    11041 gcaagccgta accgtcgacg ttcatgtgcg ggcgctcctc gctcgcgacg gagatcgctt
    11101 tcatgcggga gacgttgagc cgaacgtaaa ccgcggaacc gaactgctga acccgcggat
    11161 gcagcagaat ttcgttctgc tctggtgacg ggtaatcagg ctcatcactc tcctcgcgcg
    11221 ggacgtccag tgggggaaat agagcctgtt tctctcccca cgggccaaga atc
//
LOCUS       DASTKU010000991         7164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_639543, whole genome shotgun sequence.
ACCESSION   DASTKU010000991 DASTKU010000000
VERSION     DASTKU010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639543"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..1530
                     /locus_tag="VFQ61_35240"
     CDS             319..1530
                     /locus_tag="VFQ61_35240"
                     /inference="COORDINATES: protein motif:HMM:NF045039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959817.1"
                     /translation="MRILRFSIGKTAAVALSVSLALSTAACSDSGSNSGTGGSAPVGG
                     ASGGAGTGGTRASGGTAAGTTNSGGSTSPGGSTSGGSVSGGQGGVTTGGAATGGAATG
                     GAAGGGTAAGASGGGAGGAAGSSNGGTSAGGASSGGGGGGTSAGGATSGGAANGGMSQ
                     NGGAGGTSAGGAAGGGGTAGASGGSGGGSSGACPADAFFCSDFEEAALPAKATYSSNI
                     QTPVEVVLDKTKHHSGSQSVKVNKGSGYVYRMLSVPAAQKFWFRAYVMTDVPIDSAAK
                     VHESFMGANWGMDKGVDIVQEQCQVAININDNPVLGSNGMNPPINCPADGQKMSANTW
                     YCFEGSFDGSTGNLEIFVDGKSIIKGNAVSQAKQQFTNFRFGYVEYNGTNRTVWYDDV
                     ATGPTRIPCSQ"
     gene            complement(1587..2858)
                     /locus_tag="VFQ61_35245"
     CDS             complement(1587..2858)
                     /locus_tag="VFQ61_35245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9959818.1"
                     /translation="MTLAEQTPELPEHYFRHEFGRLVSVLSRHFGVQHLELCEDAAQT
                     ALLRALESWSRGNVPEDRGAWLYRVANNQVLDALRRNRRNEPFAPDLENEVADPAPAR
                     SDEVQLDSEVKDDLLRLLFICADPSIPAESQLVLALKTLCGFSTEEIALRLFHSQENV
                     HKRLQRARSKLRSQAASWDTPDSASLRERLPSVLQMLYLIFNEGYSSAQPDCLIRREL
                     CEEAVRLTLLLMGHPSCARPETYALLGLMYLHAARFEARIDGSGGLLLLEEQDRTLWD
                     RELIELGLYYLRRSSGETEISRYHLEALIAVEHCLAPTYAHTRWREITRLYEALDRLA
                     PSPLNLLNRAIALAEWQGPRAGLDLLESLTPPAWLLSYYLWDATLGELYRRSGDSEKA
                     IRHLIRARDAAPTHAERSLLERRLTACSGEP"
     gene            complement(2861..3214)
                     /locus_tag="VFQ61_35250"
     CDS             complement(2861..3214)
                     /locus_tag="VFQ61_35250"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9959819.1"
                     /translation="MSSPKFLFLYRTPVDAPRWSPSPEEMQAAFAQWQAWRAKFNEEI
                     VDTGDGLKPTAPAAVYKAGTVTDGPFIEAKEVMAGYSIISAKSLERAIEIARECPVNR
                     QPGASIEIRELAQYK"
     gene            complement(3330..4040)
                     /locus_tag="VFQ61_35255"
     CDS             complement(3330..4040)
                     /locus_tag="VFQ61_35255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959820.1"
                     /translation="MSTTTEWNRQRTSWRQWLFAAGLVASLAFAGCESSDDNPNGTAQ
                     GGTRGGATHSGGRAGMGGSGAADSGGASTEGGAPTNGGASAGASQGSGGHAGEHSSGG
                     GGATGGIDAACDCQFEDGSSSPACTISIAEFEKRVSLPDSCEMDLDFLERATCDDGST
                     RYTWLEGSENDFTLVFSEKGELLYGSASGYVGGLCGDSTDVWSIESGAPPVQASCERC
                     RICTGPSSAGGAPPDCEN"
     gene            complement(4156..5853)
                     /locus_tag="VFQ61_35260"
     CDS             complement(4156..5853)
                     /locus_tag="VFQ61_35260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9959821.1"
                     /translation="MGRRAILSVIGAVTLAACSDDSPTTANAAGGSGGRSSAVGGSSS
                     GGASPAKGGSGGDAGAKGGATALEPACQVGANPSSSVQKPELIATLFDRWHEAWLASP
                     AVVDLDADGVSEIIVPRDELLLIWHLDGSLVRRFELPGRIWASPVVADLDPSRPGLEL
                     AVASRQQIAAWDAQGNPLSGFPFTWRDEMRSLAAADIDGDGGLELVAVTTSDLESGGQ
                     TDILIAVHANGKVVNGFPPNTTGHSGCDDACYVHAGYDQNVALGDVDGDGIADILAPQ
                     DNSYVSLHHGDGRAFDAAPIFKGRQKFLGIRFMLDYADAQQGYAEDEENSNQAHFTNT
                     APAIVDVDRDGKNDLVMLGSVQNAAQSDRERGVVLFVVHQDGTRPAEWVKPFHAPEFL
                     DGLWDAGDNIVAATNQVTVLELDPAHAGYEFVFAGFDGKIHAVTSSNEELWQYPFTTR
                     AGVLTGGVIAADLSRDGRPEIVFNSYSTAADQGALFVLSSAGALLHRIPLPGRGAMPV
                     PTAADIDGDGQLEIIVSLKDAVDKQSQVQVYAVPGSGTNCMPWPTGRRDLRRSGLVQ"
     gene            6049..6999
                     /locus_tag="VFQ61_35265"
     CDS             6049..6999
                     /locus_tag="VFQ61_35265"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET9959822.1"
                     /translation="MKAMIIREFGGPERFESAEMPKPSPAAGQVLVRLRAASVNPVDY
                     KIRQAGSWARVPMPCILGYDAAGVVEAVGNAVTHLAPGDEVFYCARIFGRQGTYAEYH
                     VEDAEIVSRKPTHLSFEEAASLPLAAITAHDAVITFFGTKPGDTVLIHAGAGGVGAFA
                     VQLAKAAGARVIATGRRENEALIRGLGADEVIDYRFVRFEDEVNRLTNQRGVDAAYDT
                     VGGDTISRSIHCVRPYGKLATIVRVDGSINDMQIRNQTLYFGFMERTQAKIQTLATLA
                     TRGQVRPLIDSVFPLDRVADAHRKIESGGMRGKIVLKVAD"
BASE COUNT         1341 a         2228 c         2273 g         1322 t
ORIGIN      
        1 gcgggtgaag cagcagcccg ccgcaagcca gcaatcaaaa gaaagaaagt ttctaatccg
       61 ggttgatgtg ggttgcgcgc tacgggcgcg cggagttcct gctctttttg ctcagatttg
      121 cttggcgctt cggattcggg aggcccgaaa gtattcttca gaggcgcttc ggtctacgcg
      181 ggtgggtgaa accgcggatg cggagtgatc gatgagtgct atgaagcccg tgagtgctat
      241 gtgagcggta agcggcgtgg ggcttggctg ggttgaatcc gtggttgttg ttcgttggat
      301 tgataactag aggcatagat gcgtattctt cgtttttcga ttggtaagac agccgcagtg
      361 gctctgagcg tttcgctggc tttgtcgact gccgcctgca gtgactcggg gtcgaactcg
      421 ggaacaggcg gaagtgcccc agtgggcggg gcgagcggcg gtgccggcac gggtggaacg
      481 cgagcctctg gcggaacagc tgcaggaacc acgaatagcg gtggaagcac gagcccgggt
      541 ggaagtacgt cgggaggatc ggtttcaggc ggtcaaggcg gagtcaccac gggaggcgct
      601 gctaccggag gcgctgctac cggaggcgct gcgggtggcg gcacagcggc aggtgcttcc
      661 ggcggcggcg ctggtggcgc ggcggggagt tccaacgggg gaacgtctgc tggaggcgcg
      721 agttcaggcg gtggcggcgg cggtacgagt gccggcgggg ccacttccgg aggcgctgcg
      781 aacggcggga tgtcccaaaa cggtggcgct ggcggtacga gcgccggtgg cgcggcgggc
      841 ggtggcggca ccgcgggtgc ttctggtggc tcgggcggcg gcagcagtgg cgcgtgcccc
      901 gccgatgcgt tcttctgctc ggacttcgaa gaggccgcgc tgcccgcgaa ggccacctat
      961 tcttcgaaca tccagactcc ggtcgaggtc gtgctggaca agaccaagca ccacagcggt
     1021 agtcaatcgg tcaaagtgaa taagggctca ggctacgtct atcgcatgct gtctgtgccc
     1081 gcggcgcaga agttctggtt ccgcgcttat gtgatgacgg acgtaccgat cgatagcgcc
     1141 gccaaggtgc acgagagctt catgggcgcg aactggggca tggataaggg cgtcgatatc
     1201 gtccaggagc agtgccaagt cgcgatcaat atcaatgaca atcccgtgct cggctcgaac
     1261 ggtatgaatc cgccgatcaa ctgccccgcg gacggacaga agatgagcgc caatacctgg
     1321 tactgtttcg agggctcatt cgatggttcg acgggcaacc tcgagatctt cgtggacggc
     1381 aagtcgatca tcaagggcaa cgccgtctcg caggccaagc agcagtttac gaactttcgg
     1441 ttcggatacg tcgagtacaa cgggacgaac cgcaccgttt ggtacgacga cgtggcgacc
     1501 ggccccacgc gcattccctg cagccagtag tcgcactcga gataatgtcg tgcggcgcgc
     1561 accgtcgccc gccgcacgac gagaactcag ggctcaccag agcaagccgt caaacgccgt
     1621 tcgagtaggc tgcgctcggc gtgggtgggc gccgcatcgc gagcacgaat caagtggcga
     1681 atggctttct cggaatcccc cgaacgccga tagagctctc ccagtgtggc atcccacaag
     1741 tagtaactca gcagccacgc gggcggggtc aacgattcga gcaagtcgag ccccgcgcgt
     1801 gggccttgcc actcggcgag agcgatggcc cgattcaaga gattgagcgg tgatggagcg
     1861 agccggtcga gagcttcgta caacctcgtg atttctcgcc aacgagtgtg cgcgtacgtc
     1921 ggcgccaaac aatgctcgac cgcaatcaag gcctccagat gatagcggga aatctccgtc
     1981 tcaccgctgc tccgtcgtag gtagtacaac ccgagctcga tcagctcccg atcccacagc
     2041 gttcggtcct gttcctccaa caggagcaat ccgcctgaac catcgatccg cgcttcgaac
     2101 cgggccgcgt gcaggtacat caacccgagc agcgcgtagg tttcgggtcg ggcgcagctg
     2161 ggatgtccca tcagcagcag cgtgagccga accgcttcct cgcagagctc acggcgaatc
     2221 aagcaatccg gctgcgccga gctgtaaccc tcgttgaaaa tgagatacag catttgcagc
     2281 acgctcggca gtcgctcgcg aagcgacgcg ctgtccggcg tgtcccacga ggccgcttgg
     2341 gagcgcaact tgctgcgcgc tcgctgcagc cgcttatgca cgttctcttg actatgaaat
     2401 agccgcagcg cgatctcctc ggtgctgaat ccgcacaaag tcttcagcgc caagaccagc
     2461 tgtgactcag cggggatgct gggatcggcg cagatgaaca gcaagcgcaa caggtcgtct
     2521 ttgacctccg agtctaactg cacctcgtca gatcttgcgg gagccggatc cgcgacttcg
     2581 ttctccagat cgggtgcaaa aggctcgttt cgtcggttgc gccgcagagc atcgagcacc
     2641 tgattgttcg caactcgata gagccaagcg ccgcgatctt cgggtacgtt gccccgtgac
     2701 catgactcga gcgcgcgcaa caatgctgtt tgggccgcgt cctcgcacaa ctcgaggtgc
     2761 tgcacaccga agtgccgcga caacaccgag accagacgcc cgaactcgtg gcggaagtag
     2821 tgctccggaa gttcgggcgt ctgctcggcg agggtcacgc ttacttatat tgagcgagct
     2881 cgcggatttc tatgctggcc ccgggttgcc gattgaccgg gcattcgcgc gcgatctcga
     2941 tggctcgctc caaagacttg gccgaaatga tgctgtagcc ggccatcact tccttggcct
     3001 cgatgaaggg accgtccgtc acggtaccgg ctttgtaaac cgcggcggga gcggtgggct
     3061 tcaaaccgtc cccagtatcg acgatttcct cgttgaactt ggctcgccaa gcctgccact
     3121 gcgcgaaggc cgcttgcatc tcctcgggag aggggctcca acgcggggcg tcgactggag
     3181 tgcgatagag gaacagaaat ttggggctgg acatgtttct tggacctttc cttcaggccg
     3241 accaggtttt ggtgcgggct cgctcgaacg acgcagctac cccgacgact tggacacgcc
     3301 ttcgccactt tttcgttttt tgcgtccggc taattttcac agtccggagg ggcgccgccg
     3361 gcggaggatg ggccggtgca aatcctgcat cgttcacagc tcgcctggac cgggggagcg
     3421 ccgctttcga tggaccagac gtccgtcgag tccccgcaga ggccacccac gtagccggag
     3481 gcagatccgt acagcaactc gcccttctcc gagaagacca gtgtgaagtc gttttctgag
     3541 ccctcgagcc aggtgtaacg ggtgctgccg tcgtcgcagg tggcgcgttc caggaaatcg
     3601 agatccattt cgcaagaatc cggaagcgac acgcgcttct cgaactccgc gatggagatg
     3661 gtgcacgccg gggatgacga gccgtcctcg aactgacagt cgcacgccgc atcgatcccg
     3721 cccgtcgcgc cgcctccgcc cgaggagtgc tcaccggcgt gaccaccgga gccctgactt
     3781 gcacccgcgg atgcgccacc gtttgttgga gcaccacctt cggtggaagc gccgcctgaa
     3841 tcggctgcgc cggagccacc cattcccgct cgtccaccgg agtgcgtcgc tcccccgcga
     3901 gtgcccccct gcgcagtacc attcgggttg tcatcggaag actcgcagcc ggcaaaggca
     3961 agggacgcga ccagccccgc cgcgaatagc cactgtcgcc acgatgtccg ttgcctgttc
     4021 cactcagtgg tcgtgctcat cggctggaac gatagcggca cggaacatgg ctttcaaggc
     4081 actctgaatg cgggtcgaga gccaaagccg agcttcgcga accgacgtct cacagcgaag
     4141 tttctgagcg tagtactact gcaccagccc gctgcgcctc aggtcccggc gccccgtggg
     4201 ccaaggcatg caattcgtac ccgaaccagg caccgcatac acttggacct gactctgttt
     4261 atcgaccgca tccttcaggc tcacgatgat ctcgagctga ccatcgccat cgatatcggc
     4321 cgccgtaggc acgggcatgg cgccgcgacc cggtagcggt attcgatgca gcaacgcgcc
     4381 cgcactcgag agcacgaaca gagccccttg gtccgcggca gtcgagtagc tattgaatac
     4441 gatctcaggg cgaccatcgc gcgataaatc cgccgcgatc actcctcccg tgagcacgcc
     4501 cgcacgcgtg gtgaatgggt attgccacag ttcttcgttg ctcgaggtta ccgcgtgaat
     4561 tttgccatcg aagccagcaa acacgaactc gtagcccgca tgcgcgggat cgagctccag
     4621 caccgtaact tggttggtgg cagccacgat gttgtcgccc gcgtcccaca agccgtccaa
     4681 aaactccggc gcgtggaatg gcttgaccca ctccgccgga cgagtgccgt cttggtgcac
     4741 gacgaacaac acgaccccac gctcccggtc ggactgggcc gcgttttgta cggatccaag
     4801 catcacgagg tcattcttcc cgtcccgatc gacatcgacg atggccggag cggtgttggt
     4861 gaagtgggct tggttcgagt tctcttcgtc ctcggcatag ccctgttggg cgtccgcgta
     4921 gtcgagcatg aagcgaatgc cgaggaactt ctgacgtccc ttgaagatag gagccgcgtc
     4981 gaaggccctg ccgtcgccgt ggtgcaggct cacgtacgag ttatcttgag gcgccaagat
     5041 gtccgcgatg ccgtcgccgt cgacatcgcc caaggcgacg ttttggtcgt agcctgcgtg
     5101 cacgtagcat gcatcgtcgc agccgctgtg tccggtggtg ttgggaggaa acccgttcac
     5161 gacttttccg ttggcgtgga cggcaatcaa aatgtcggtc tgtccgccgg attcgagatc
     5221 gctcgtggtc accgcaacta actcgagccc tccatcgcca tcgatatcag ccgccgcgag
     5281 cgaccgcatc tcatcacgcc aggtaaatgg aaaacccgag agcggattgc cctgcgcgtc
     5341 ccacgccgca atctgctggc gactcgctac tgccagctcg agccccgggc gactggggtc
     5401 gagatccgca accacggggc tcgcccagat acgcccgggc aactcgaagc ggcgcacgag
     5461 cgacccgtcc aggtgccaaa tcagcagtag ttcgtcccgg ggcacgatga tttcggagac
     5521 gccatcggcg tccagatcca cgaccgcggg agatgcgagc cacgcttcgt gccaacggtc
     5581 gaacaacgtc gcaatcagct caggcttttg cactgaactc gaagggttcg cgccgacctg
     5641 gcaggcgggt tcgagtgcgg tcgccccacc cttcgctcct gcgtcaccgc cgctcccgcc
     5701 tttggctggt gaagcgcctc cggaagagct gccaccgacc gcgctgctcc gcccgccgga
     5761 gccgccggct gcgtttgccg tagttggtga atcgtcacta cacgccgcca aggtgaccgc
     5821 gccaatcaca gacaagattg cccttctgcc catgcagcta tgctctcgca atgcggggcg
     5881 cttcggtagt gctttgcacg catgggctga cagtgtttct agtagtgcga tgtccggtgg
     5941 cgtgcgccgc ggccccagcc ctgatgccaa agagactgcg cggctcagaa cctcttacgt
     6001 ccgcgacggc gctgcggtac cgtgcgagcg ctccaggaga tcgccgccat gaaagccatg
     6061 atcattcgag agttcggggg cccggaacgc ttcgagagtg cggagatgcc gaagccctcc
     6121 cccgccgcgg ggcaggtgct cgttcggcta cgagccgcct ccgtgaatcc cgtcgactac
     6181 aagattcggc aagcgggcag ctgggcgcgt gtacccatgc cttgtatttt gggttacgac
     6241 gccgccggcg tagtggaagc cgtgggaaat gccgtgaccc acttggctcc tggggacgag
     6301 gtcttttact gcgcgcgcat cttcggacgg cagggcacgt atgccgaata ccacgtcgaa
     6361 gacgccgaga tcgttagccg gaagccgacc cacctgagct tcgaagaggc cgcgtcattg
     6421 ccgctcgctg cgatcacggc tcatgacgcg gtcatcacct tttttggaac caagccggga
     6481 gacacggtgt tgatccacgc cggagcgggg ggcgtgggtg ccttcgccgt gcaactcgcc
     6541 aaggccgcgg gtgcgcgcgt gatcgccacg gggcgacgcg agaacgaagc actgattcgc
     6601 ggattgggcg ccgacgaggt gattgactat cgattcgttc gcttcgagga cgaggtcaat
     6661 cgcctgacga accaacgtgg agtagacgcc gcctacgaca cggtcggcgg agacacgatc
     6721 tctcgcagca ttcactgcgt gcgcccctac ggcaagctcg ccacgatcgt gcgtgtcgac
     6781 ggcagcatca acgacatgca gatacgaaat cagaccctgt acttcggctt catggagcgc
     6841 acgcaggcca agatccagac cctcgcgacc ttggccacac ggggacaagt gcgccccctc
     6901 atcgacagcg tcttccctct cgatcgagtc gcggacgctc accgcaagat cgagagcgga
     6961 gggatgcgcg gcaagatcgt gctcaaggta gcggattgag cgcaagacag cataccctga
     7021 gtggatatgt gccccccttc gttgtgtctg cggcgacgcc ctgccgcggc agagtttaat
     7081 tcgccgtcgc agcctgcaac gggctgcgat cgaggctgcc cgcctcgagc ccgagcacgt
     7141 gctcgtaaac tcggctcgtg cctt
//
LOCUS       DASTKU010000992         5771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1757273, whole genome shotgun sequence.
ACCESSION   DASTKU010000992 DASTKU010000000
VERSION     DASTKU010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757273"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1512)
                     /locus_tag="VFQ61_35270"
     CDS             complement(250..1512)
                     /locus_tag="VFQ61_35270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959823.1"
                     /translation="MSPFRTSRWGYVAGCAIQGAALLSAMGCSAPAARLRVFSELDSV
                     RESPAVQEAAQTAPQAYANAQRLRLEAERAEKAGDTAGAQILGESSLAAYEHAAILAR
                     LGRAEQARAKAESRLAGATAELSELSAQEQRVRAETEALEMKARIARETSPSPELQKG
                     TPEREKARREAARSLLLQARLLCVSARLVEPARAGVAPLLNELDQLEKPARTAARAPQ
                     GLSPASPQTAAGSPSPTSTGSSTDVRSAQDGATTKPSPAGYFEQAVRLRASCLSELSQ
                     ARRTVSLSRPASAEADRLLTELSQAAYSPSRDDRGVAVTLRGAFGKGAELSAEGLSSL
                     QALSRVAAAHPEFPILVVLHSANQPNEGLERQRLAQVQAALQRAGATSSIDIAWAADA
                     APLIAPQRAGAAERNQRLEVVFVAKSAS"
     gene            complement(1509..2267)
                     /locus_tag="VFQ61_35275"
     CDS             complement(1509..2267)
                     /locus_tag="VFQ61_35275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959824.1"
                     /translation="MSSRNKTALHCLSCDATLPNYRPNPAQQPVALQAGAAPTGVPLA
                     KPWLPRMGHAGLFCTLLLAMASAAHAETTPRAQAEATVRELEGSQGKDRALTEPLLVR
                     ARSALTRAKNARQAGDQRHGSLLEALAMELAESARDLVRVKSAEQQTTEFEKQALDAE
                     TRAVRARALVELAAARRGRAQEQLDEAEAARRTNAKADGAASNKSEGKPPVTPRLTAP
                     RPAETPKPTQTDKPLQPKPTQAPAGQPAGVRGQP"
     gene            2375..2863
                     /locus_tag="VFQ61_35280"
     CDS             2375..2863
                     /locus_tag="VFQ61_35280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HET9959825.1"
                     /translation="MVERSRASWDEYFMAIAKVVATRSTCDRKHVGAVVVRERMILAT
                     GYNGSIRGLAHCDDAGHMMEGGHCVRTVHAEANAIVQAARNGIRLEGAAIYVTASPCF
                     GCFKLIANAGLSRIVFGEFYRDERIYALAQELKIELKLLDANTIERMADSNSTEIATR
                     SE"
     gene            complement(2878..3777)
                     /locus_tag="VFQ61_35285"
     CDS             complement(2878..3777)
                     /locus_tag="VFQ61_35285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959826.1"
                     /translation="MTELVVHVVRPYSSEAEYLDAEAWTIDARGMLLVDQPELPVGAI
                     IQFDVSLAQGQKVMRGEARVAAYLAPTEDQVGAVRVRFRKYAQQTKAFIDRALAAHER
                     QLAELASEPAPAPTASIAAPPAASAPAAVDPSTAQTTPLNAASPLNAASPLNQPAAKA
                     AEPGNQTPLNATAPAAPVNAPAPRSAASQSAGPHSFTEPAQTLPSEQRIAKPQLEPAA
                     ADICRPAYSDPIEPSGIHRRPVPYAPPVAPPENREELLARLRERAERKRADERARRDP
                     TTLDVREAGPSGLSEEPRRSRTS"
     gene            3882..5225
                     /gene="purD"
                     /locus_tag="VFQ61_35290"
     CDS             3882..5225
                     /gene="purD"
                     /locus_tag="VFQ61_35290"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET9959827.1"
                     /translation="MSSLHNSSLDNSSLRKVLVVVSGGREHALAYHLLSAPSVAEVIV
                     APGNEGTDHTPTDVTGKVLRNAPGDVLELARRERPDLVVIGPEAPLCAGLTDELLSAG
                     LVTFGPSRAAARLEGSKAFMKEFVTRHGIPTAAHVVVRSLAELEPALARFGQAPVVKA
                     DGLCAGKGVVVADTHEEARLAAQAMLSGESFGDAGRVVVLEERLVGAEASVHAICDGE
                     RFWVLPPAQDHKRIFDADRGPNTGGMGTYAPAPLVDPAVSRRVEERILAPTLRAMAAE
                     GHPFRGTLFAGLMIAPDGEPRLMEFNVRFGDPETQVMLGLLNGDLAETLIQAANGNLV
                     TRGLTLKSASSVCVVLAAPGYPEAPHTGSVISGLETASKLPGVVVYHAGTRRDASGQV
                     VTSGGRVLGVTAVGDSLVQAHSRAYAAVREIRFDGMQYRSDIAARALPNPAVPVR"
     gene            5377..>5771
                     /locus_tag="VFQ61_35295"
     CDS             5377..>5771
                     /locus_tag="VFQ61_35295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET9959828.1"
                     /translation="MARVINFNAGPAALPLPALERAQKELLDLGGTGMSVMEHSHRGK
                     AYEAVHNEAIGLVRELMNVPENYDILLLQGGASQQFAVVPMNLLPQGKSADYILTGAW
                     SQKAYKEAKTLGTVRVAGTTEKDKKFARIP"
BASE COUNT          963 a         1838 c         1907 g         1063 t
ORIGIN      
        1 tctggaaaca cgcagactgg aaaccagatc ccgtgctccg aagactcgct ggatccagag
       61 cgctcgagtg caaacgcgca gtcacgaggc tctgagtcca atcccacaga gctgcaccga
      121 ggtgtgtgct cgcgtggcgt cacgagaggc ggtctcacgc aagcacgcga ttcgagcgtc
      181 gcggttcgcg cgactcggag atagggagga agctttaaat agcctgtcgg tttcggggcg
      241 ccgctcgact caagaggcgc tcttggcgac gaaaaccacc tccaagcgct gattgcgctc
      301 ggcggctccg gctctttggg gagcaatcag cggcgccgca tctgctgccc atgcaatatc
      361 gatcgagctc gttgcgccgg cacgctgtag ggctgcctgc acctgcgcca gacgttgccg
      421 ctcgagtcct tcgttgggtt gatttgccga gtgcagcacc accaaaatcg ggaactcggg
      481 gtgagccgca gcaacgcggg ataacgcctg tagggaggaa agaccctccg cactcaactc
      541 ggcgcccttt ccgaacgcgc ctcgtagcgt cacggcgact ccgcgatcat cgcgcgaagg
      601 agaataggcg gcctgtgaga gctcggtcag cagacgatcg gcttcggctg aagcgggtcg
      661 gctcaaggag acggtgcgtc gggcttgcga gagctcactt aggcaactgg cgcggagcct
      721 cactgcttgt tcgaagtagc cagcgggcga aggtttcgtc gtcgcaccat cctgcgcgga
      781 tcgaacatcc gtgctcgatc cggtcgacgt gggggagggg gatcctgcgg ctgtctgggg
      841 tgaagccggg ctgagccctt gcggcgctcg cgcagccgtc ctggctggtt tttcgagctg
      901 atccagttcg ttcagcagcg gcgccacgcc ggcgcgcgcg ggctcgacca agcgcgccga
      961 gacgcacagc aaacgcgcct gcaacagcag cgaacgagcg gcttcgcgtc gcgccttttc
     1021 acgttctggc gttcccttct gtagttccgg agaaggggag gtctcgcgcg cgatgcgggc
     1081 tttcatttcc agcgcctcgg tttccgcccg cacgcgctgc tcttgagcgc tgagctcgga
     1141 tagctccgca gtggcaccgg caaggcggct ctcggccttt gccctggctt gttcggctcg
     1201 tcccaagcgc gcgaggatcg cggcgtgttc gtaagcggcg agtgagctct ctcccaaaat
     1261 ttgcgcccca gccgtgtcgc ccgccttttc tgcacgttcg gcctcgaggc gtagtcgttg
     1321 ggcgttcgcg taagcttgcg gcgcagtctg cgccgcctcc tggaccgcgg ggctctcgcg
     1381 cacggagtcg agttcgctaa acacccgcag tcgcgccgca ggcgctgagc aacccatggc
     1441 tgaaagcagc gctgctcctt gaatggcgca acccgcaacg tagccccaac gagaagtccg
     1501 aaacgggctc atggttgacc tcgcacacct gcgggttggc cagcgggcgc ttgcgtcggt
     1561 ttcggctgaa ggggcttgtc agtttgcgtt ggctttggcg tctcagctgg cctcggcgcg
     1621 gtcaagcgag gtgtcaccgg cggcttgccc tcggacttat tgcttgcagc gccgtccgct
     1681 tttgcgttcg ttcgtcgcgc ggcttcagct tcatcgagtt gctcttgagc acgcccgcgc
     1741 cgggctgccg caagttccac caaggcccgg gcacgaacgg cgcgcgtttc cgcgtccaag
     1801 gcttgcttct cgaactcggt cgtctgttgt tcggcgctct tcacacgaac taggtcacgc
     1861 gcgctctcgg ccagctccat ggccagagct tctagcaagc tgccgtggcg ctggtctccc
     1921 gcctggcggg cgttcttggc tcgtgtgagc gcgcttcggg cacgcacgag cagcggctcg
     1981 gtgagcgccc ggtccttgcc ttgggagccc tcgagttctc gcactgtggc ctcggcttgg
     2041 gctcgaggag tggtctccgc gtgcgccgcg ctcgccatcg cgagcaagag cgtacaaaac
     2101 agacccgcgt gacccatgcg cgggagccac ggcttggcga gcggcacccc ggtcggggcc
     2161 gctccggcct gcagcgccac cggttgttgg gcagggttcg gacggtaatt gggcaacgta
     2221 gcgtcgcagc tcaggcaatg aagagcagtc ttgttccgag aactcacgcc ggaaacaagc
     2281 caccgaagcc gcggcgggtc aagcttggac tccgggtgcg tcaaaggcgg ggcaagccac
     2341 gaacgagggc cgaatctgcg ctataagcgc gcccatggtc gagcgaagcc gcgctagctg
     2401 ggacgaatac ttcatggcga tcgcaaaggt cgtcgcgacg cgctctacgt gcgatcgaaa
     2461 gcacgtcggc gcggtcgtcg tgcgcgagcg gatgatcctg gcaactggct acaacggctc
     2521 gattcgtggc ctcgcgcact gtgatgacgc gggccatatg atggagggtg ggcactgcgt
     2581 gcgaaccgtc cacgccgaag ccaacgccat cgtccaagcg gcccgcaacg gcatccgcct
     2641 agagggcgcc gcaatttacg tcacggcttc gccttgcttt ggctgcttca agctgatcgc
     2701 gaatgccggc ctctcgcgca tcgtgttcgg cgaattctat cgagacgagc gcatctacgc
     2761 gctcgcgcaa gaactcaaga tcgagctcaa gctgctcgat gccaatacca tcgagcgcat
     2821 ggcggacagc aactcgaccg aaatcgcgac ccgttccgaa tgaacgcggc ccgcaaatca
     2881 ggaggttcga gagcggcgcg gctcttcgct cagcccgctc gggcctgctt ctcgaacatc
     2941 gagggtagtg gggtcgcggc gagcgcgctc atccgcacgc ttgcgctcgg ctcgctcgcg
     3001 caagcgcgcc agtagttctt cacgattttc tggcggagct acgggcggcg cataaggcac
     3061 tggtcggcgg tgtatcccgc tcggttcgat cgggtcggaa taggctgggc gacagatatc
     3121 cgcggctgcg ggttctagtt gcggcttcgc aatgcgctgc tccgacggca gagtctgggc
     3181 tggttcggtg aatgagtgcg gtcccgccga ctggctcgcc gccgatcgag gggcaggagc
     3241 attcacgggt gctgccggcg cggtggcatt caacggcgtc tggttccccg gttctgccgc
     3301 cttcgccgct ggttgattca acggcgaagc cgcgttcaac ggcgaagccg cattcaacgg
     3361 agttgtctgc gcggtgctcg gatccaccgc ggcgggcgcg ctcgcggcgg gcggcgccgc
     3421 gatgctcgcg gtcggggccg gcgcaggttc tgacgcgagc tctgcgagct ggcgctcgtg
     3481 cgcggcgagc gcgcgatcga tgaatgcctt cgtctgctgg gcgtacttgc ggaaccgcac
     3541 ccgtacggcg cccacctgat cttctgtcgg tgctagatac gcggcgacac gtgcttcgcc
     3601 acgcatcacc ttttgccctt gcgccaacga aacatcgaac tgaatgatgg ccccgacggg
     3661 tagctcgggt tggtctacca gcaacatacc ccgcgcgtcg atcgtccaag cctccgcatc
     3721 gaggtactcc gcctcgctgc tgtagggcct gacgacgtgc acgacgagct ccgtcataac
     3781 ggtgattgga tgatagcccg tctattcgtg ggtgagccac aaatcgccag ggttttctgg
     3841 ttgacgctcg acgaaaggtc cggcatccca cacccgcgtt catgagctca cttcacaact
     3901 cgtcactcga caactcgtca ctccgtaagg tcctggttgt tgtctccggc gggcgcgagc
     3961 acgcactcgc atatcatctg ctgtccgcgc cctcggtggc ggaggtgatc gtggctccgg
     4021 gcaacgaggg caccgaccac actccgacgg atgtgaccgg caaagtgcta cgcaatgcgc
     4081 ctggcgacgt gctggagttg gcgcgccgag agcgccccga tctcgtagtc atcggacccg
     4141 aggcgccgct ctgcgctggg ctcaccgacg agctcctgag cgcaggattg gtgacgttcg
     4201 ggccaagccg cgccgccgca cgcctggagg gttccaaggc gttcatgaag gagttcgtca
     4261 cccggcatgg cattcccacc gcggcccacg tcgtggtgcg aagcctcgcc gagctcgagc
     4321 cggcgctcgc gcgcttcgga caagcgcccg tcgtcaaggc cgatggctta tgcgccggca
     4381 agggcgtggt agtggcggac acgcatgagg aagctcgcct cgcggcgcaa gcaatgttgt
     4441 caggagagag cttcggagat gccggtcggg tcgtggtgct cgaggagcgg ctggttggcg
     4501 ccgaggcgag cgtgcacgcg atctgcgacg gcgaacgctt ctgggtgctg cccccggcgc
     4561 aagatcacaa gcggatcttc gacgcggata ggggaccaaa caccggcggc atgggaacgt
     4621 acgctcccgc accgttggtc gaccccgccg tctcgcgtcg tgtagaagaa cgtatcctgg
     4681 ctcccacgct gcgcgctatg gccgcggaag gacatccatt tcgaggcacg ctgttcgctg
     4741 gcttgatgat tgcgccggat ggggagccac gcctgatgga attcaatgtt cgcttcggtg
     4801 acccggaaac ccaggtcatg ctcggtctgt tgaacggaga cttggcggag accctgattc
     4861 aggctgcgaa cggcaatctg gtaactcgag gtctgaccct caagagtgca agctcggtgt
     4921 gcgtggtgct cgcggcgccc ggctatccgg aggctccgca caccgggagc gtcatctccg
     4981 gattggagac cgcctccaag cttccgggag tggtcgttta tcatgcgggc acgcggcgcg
     5041 atgcctccgg tcaggtggtc acttcgggtg gacgcgtgct tggagtaacg gcggtcggag
     5101 actcgcttgt tcaggcgcat tctcgcgcgt acgccgccgt tcgggagatt cggttcgacg
     5161 gcatgcagta tcgctccgac atcgcggcgc gagcgctgcc caaccctgct gtaccggtgc
     5221 gctaacgccc ccgagcgagc tagtgcttga cgcactgcgc agccggggct aatgcagcag
     5281 ccgcaaattc cgcccgcttc cgactttacg aggaatccac ttgcgacgtc ggtggcgcgg
     5341 cactagcccg acgcttcatt cgacattcgg aggaccatgg ctcgagtaat caatttcaac
     5401 gcaggccccg cagctctccc tcttcccgcc ctcgagcgag cccagaagga actgctcgac
     5461 ctcggcggaa caggcatgag cgtgatggag catagccatc gcggcaaagc ctacgaagcc
     5521 gtgcacaacg aggccatcgg gctagttcgc gagctcatga atgttccgga gaactacgac
     5581 atcctgttgc tccagggtgg cgcgagccag cagttcgccg tggttccgat gaacctgctg
     5641 ccccaaggca agagcgccga ttacatcctg acgggtgcct ggtcgcagaa ggcctataag
     5701 gaggccaaga cgctcggtac ggtgcgcgtc gccggcacca ccgagaagga caagaagttc
     5761 gctcgcatcc c
//
LOCUS       DASTKU010000993         6872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1982324, whole genome shotgun sequence.
ACCESSION   DASTKU010000993 DASTKU010000000
VERSION     DASTKU010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982324"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFQ61_35300"
     CDS             complement(<1..500)
                     /locus_tag="VFQ61_35300"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET9959829.1"
                     /translation="MTTANSSPTPSQAVPAVKRPLEPAHIIRSDEEAIAVATELAARF
                     AQGAQERDRVRRWPLEELDAFSQSGLWSINVPKAFGGPELSYVTLSRVIEIVSAADPS
                     LGQIPQNHLGVVAAIRTVSDLEQQRLLFGEVLKGRRFGNAFSEFGSKRAVDFETRFRD
                     AGDHVLV"
     gene            complement(739..2625)
                     /gene="recD"
                     /locus_tag="VFQ61_35305"
     CDS             complement(739..2625)
                     /gene="recD"
                     /locus_tag="VFQ61_35305"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HET9959830.1"
                     /translation="MITLDLQSWQDAGILSEADIEFSNTLSELSPGELAPEVRLGAAF
                     SAWAIRNGHTCASLPALSDKIWYDEEGHPLELSLPGVSTWRSELERSPLVIRAFESAE
                     AVPPPAVAPLVLAAGERLYLTRYFKYERLLVESLLARATRLPELADGHRLRAGLEHYF
                     PASDPKVEDQRTAALLSLLRSFALISGGPGTGKTFTTAKVLMLLQEQALLGGRPPLEI
                     LLLAPTGKAAQRLGEAIRKNLEQLSCPERVRDIIPKEASTIHRALGFQPRSPTQFKHN
                     RANPLPYDVVLVDEASMVDVALMAKLFDAVPEASRLVLLGDKDQLASVEAGAVLGDIY
                     GEREHDGYSRVFTAHARQLTGTELPESPTRPQAGLHDCLVHLRHSHRFAEGGSIANVA
                     TRIKNGDCDWIEAAASGDLPGVELRPTLTRANFDALLGRSLLEHFGDLADLGPLDQLS
                     RLDGFRLLCCHRRGPASVEELNAAVVRYLQKRGRLEANSDYYAGRPVAVTTNDYQLEL
                     FNGDIGVLSATPSDATRTHLLAYFPGKEPGSLRRFPVGRLPPHETVFAMSVHKSQGSE
                     FREIALMLPEYVSPIVTRELVYTAVTRARHKVVIYGAPEILRKAIEARVLRASGIPTS
                     LWAR"
     gene            complement(2622..6248)
                     /gene="recB"
                     /locus_tag="VFQ61_35310"
     CDS             complement(2622..6248)
                     /gene="recB"
                     /locus_tag="VFQ61_35310"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HET9959831.1"
                     /translation="MKTDMAPLYPLSVELAVARGGGTLVEASAGTGKTYTITSLFLRL
                     LLERRLDPSEVGVVTFTEAATVELGERVRARVEDALHWLKRDPREAEPEDAFLKELLQ
                     RRIRERSSDLRHLERCRGDLDQTPISTIHGFCQRVLKQYAVGVRLGFDAELVPDLDEL
                     RDDILYDFWHTHVASRPLEFARQLDELGLTPKRLAELLRYVQQNPNVTLVPEARVVTE
                     QALEEAFAAAELAYRSIDLPGFLGEYVYVSRISGLSSQRRDRLCRTLDRVFKERQVFA
                     PQDSDALFPETLGEALKKKVPLPEFFFAFERFARLAFERGHELQHRLAHYARREFRIR
                     KDRRSVLGFEDLLQRLHEALHSERGPLLAGSLRDRFKALLIDEFQDTDPIQFGIFETI
                     YRDTGLPWFLIGDPKQSIYAFRGADVFTYLRASENARRFTLDTNFRSDPSMVRAVNIV
                     FCAEDAFANPHISYRPVQARPGAADALSGPEPHDRAGLEFLWVEAAPGGKSEPRPLTL
                     RRMAQIVASDIAALLARRATANSRELTELDIAVLTRTNDQCFIVQDALRNVGIHAVVI
                     GDKSVFDSDEARELALVLGAALDPSNNYELRRALSTRLFGVSAEVIAELDTNDSGFYE
                     YAEPFRRFHKLWTEHGFVRMFRPLFDEIQTRESLAYDAGGERCLVNLLHLGELLHRAS
                     VTQQLGPAALVRWFAQQRRDRPRGDESEIRLESDELAVRVLTVHKSKGLEFPVVYCPY
                     LWCSNGPRDRPGPLPFHDEQGRAFLDLEVFASERRKQSQERAKHEQLAEELRVAYVAL
                     TRAKHKTCVLWGQFSDLKNATATRLFHAELLKRGADLAKTPAADLKTDLARLAKEANG
                     AAWLREWLETPAGADERRDQLSSVPLSAREVQLAVRGWQRTESFTSLIRNNPQLRNDE
                     EAAKDHDAHAPTVNGPQASPNESSADTSPGEPILLLEFPRGRRTGDFFHELLEHLDFR
                     ADLATFAPTISEQLAAFGLLAEVDQAQRHRLSERVLATLGSVLDTPLGGFCLRDISLA
                     DRLNELEFRLPVALEKEAGQARESVTRQRLARAFREHPSPELAQGYADRLAQGYLPGL
                     RGYLKGYVDLVFRHGERWYLVDYKTTHMGDYVEDYGPAHLLQEMDRAHYYLQYHLYTL
                     ALDRYLSRYQPGYQYETHFGGVLYLFLKGMRPARGLSTGVFFEKPPVARLTALSSILT
                     GDSV"
     gene            complement(6245..>6872)
                     /locus_tag="VFQ61_35315"
     CDS             complement(6245..>6872)
                     /locus_tag="VFQ61_35315"
                     /inference="COORDINATES: protein motif:HMM:NF037683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959832.1"
                     /translation="LRIALATQKARGGPAEPVLRLQLPLAGVTLTGSIDARFQAARVE
                     HGYSSSGPKQLVKLGVKHLAMCAAGAPSPSVAVLRGQKQQSVDTIRLNPLGPDLARAR
                     LETLIHWFAKGQALPLPFLPVPSQAYFLALRGKKPEEALDKAIKAYAESNRTGIVDQH
                     VERIFRGLMPPFDSAYDQGCKSLEETHFHAASLAFFDCFQDLLGAHES"
BASE COUNT         1287 a         2108 c         2207 g         1270 t
ORIGIN      
        1 accagcacgt gatcccctgc gtcccgaaag cgcgtctcga aatcgacggc gcgtttcgag
       61 ccaaactcgg agaaggcgtt accgaagcgg cgccccttca gcacctctcc gaacagcagt
      121 cgctgctgct cgagatccga aacggtgcgg atcgcggcta ccacacccaa atggttctgc
      181 gggatctggc cgagcgaggg atcggccgcg gagacgatct cgatgactcg gctcaacgtg
      241 acgtaggaga gctcggggcc accgaacgcc ttgggcacgt taatggacca gagtccactt
      301 tgcgagaacg catcgagctc ttccaacggc cagcgccgga cgcgatcccg ctcttgtgca
      361 ccctgcgcga agcgcgccgc gagctcagtg gctaccgcaa tggcttcttc atcgctgcgg
      421 atgatatgag ccggctcgag agggcgtttg actgcgggta ccgcttgcga cggcgtcggt
      481 gacgaattgg ctgtagtcat tgtgaactct gcctcatcgc gagcacaggg tgtgccactc
      541 gatcccttac ggtattcacg gccctcgaga ggtttcgttg ctgaggaggt gttgctgccg
      601 cagcagccac tgctggcccg ttgctcgcct ccgcgcagcc cgccgcggca cccggtgcgt
      661 ggcgggggcc aggtgcgcgc cattgaagtc catgtctcgt tgtctagtat gatggtcttg
      721 gctcttgcaa agctccggct accgtgccca cagcgaagtc gggatgccgg aggcgcgcag
      781 cacccgagct tcgattgcct tccgcaagat ctcaggtgca ccgtagatca cgaccttgtg
      841 ccgcgcgcgc gtgaccgccg tgtacacgag ttcgcgcgtt actatgggcg aaacatactc
      901 tggcaacatc agcgcgatct cacgaaactc ggagccttga gacttgtgta cgctcatggc
      961 gaacacggtt tcgtgcgggg gcaagcggcc gacggggaac cggcgcaacg agccgggttc
     1021 tttgcccggg aagtacgcga gcaggtgcgt tcgggtcgcg tccgacggcg tggcagacag
     1081 cacgccgatg tcgccattga acaactccag ttgatagtca ttcgtggtca ccgcgacggg
     1141 acgcccggcg tagtagtcgc tgttcgcttc cagacggccc cgcttttgga ggtaccgcac
     1201 cacagccgcg ttgagctctt ccacggatgc aggacctcga cggtgacaac agagcagccg
     1261 aaaaccatcc agtcgggaga gctgatcgag agggccgaga tcggccaagt ctccgaagtg
     1321 ctcgagtagt gagcgcccca gcaaagcgtc gaagttggcg cgcgtgagcg taggtcgaag
     1381 ttccacccca ggtaagtcac ccgatgccgc ggcttcgatc caatcacaat ccccgttctt
     1441 gatgcgggtt gcgacgttgg cgatactgcc gccctccgcg aagcgatggc tgtgccgaag
     1501 gtggaccagg caatcgtgca agcctgcttg tggtcgcgtg gggctctcgg gcagctccgt
     1561 ccccgtgagc tgacgggcgt gagccgtgaa tactcgcgag tagccgtcgt gctcgcgctc
     1621 accatagatg tcaccgagca cggcgccggc ttcgacggac gcgagctggt ctttgtcacc
     1681 gagcaacacc agtcgcgatg cctccggcac cgcgtcgaag agcttggcca tcagggccac
     1741 gtccaccatc gaggcctcat cgacgagcac gacatcatag gggaggggat tcgcacggtt
     1801 atgcttgaac tgcgtaggcg agcgcggctg gaaacccagc gcgcggtgaa tggtggacgc
     1861 ctccttcgga atgatatctc ggacacgctc aggacacgac agctgctcta agttctttcg
     1921 gatcgcttca ccgaggcgct gcgccgcctt tcccgtggga gcgagcaaca aaatttcgag
     1981 cggcgggcgc cctcccagca aggcttgctc ttgcagaagc atcaacacct tggcggtggt
     2041 gaacgtcttg cccgtccccg ggccacccga gatcaaggcg aagctgcgga gcaggctcag
     2101 gagggcggcc gtgcgctggt cttccacctt ggggtcgctc gccggaaaat agtgctctag
     2161 tccggctcgc agcctgtgcc catcggcaag ctctggcaaa cgagtggctc gtgccagcaa
     2221 gctctccacc agcaatctct cgtacttgaa atagcgcgtg agatacagcc gctctcctgc
     2281 cgccaacacc agaggggcta ccgctggcgg tggtaccgcc tcggcgcttt cgaaggcccg
     2341 tatgacgagc ggactgcgct cgagttcgct ccgccaggtc gaaacgcctg gtaggctgag
     2401 ctcgagcggg tgcccttctt cgtcgtacca gatcttgtcg ctcaaggcgg gtaacgacgc
     2461 acaggtatgg ccattgcgga tggcccacgc ggagaacgcg gcgcctagcc tgacttccgg
     2521 cgccagctcg cccggggaca gctcggacag cgtattcgaa aactcgatat ccgcctcgga
     2581 caatataccc gcgtcctgcc acgactggag atcgagcgta atcataccga gtctcccgtc
     2641 aaaatgctcg aaagcgcagt gagccgagcc accggcggtt tttcgaagaa caccccggtc
     2701 gacaacccgc gcgctggacg catgcccttc aagaacagat acaagacccc gccgaagtgg
     2761 gtctcgtatt ggtagcccgg ttggtagcgg ctgagatagc gatcgagggc cagagtatag
     2821 agatgatatt gcaggtagta gtgggctcga tccatctctt ggagcaggtg ggcggggccg
     2881 tagtcttcca cgtagtcgcc catgtgagtg gttttgtagt cgacgagata ccagcgttcg
     2941 ccgtgccgaa agaccaagtc cacgtagccc ttcagataac cgcggagacc gggcaagtaa
     3001 ccctgtgcga gccgatccgc gtagccttgg gcgagctcgg ggcttgggtg ctcacggaaa
     3061 gcacgcgcca gacgttggcg cgtcacactc tcgcgagcct gaccggcttc cttttcgagc
     3121 gcgacgggca gccggaactc gagctcgttc aggcgatccg ccagcgagat gtcccgcaag
     3181 caaaatccgc cgagcggcgt atccagcacg ctgccgagcg tagccagcac gcgctcactc
     3241 agccgatgac gctgcgcctg gtccacctcc gccaagagcc caaaagccgc gagttgctcc
     3301 gagatagtcg gcgcaaaggt cgctagatcg gcgcggaagt ccaagtgctc caagagctcg
     3361 tgaaagaagt cgcctgtccg acggccacgc ggaaactcga gcagcagaat cggctccccc
     3421 ggagacgtgt ctgcactcga ctcattcggg ctcgcttgcg ggccattcac ggtcggggcg
     3481 tgcgcgtcgt gatctttggc ggcttcctcg tcattgcgga gctgcggatt gttgcgaatc
     3541 aaagaagtga agctctcggt acgctgccag ccccgcactg cgagttggac ctctcgcgcc
     3601 gagaggggca cgctcgagag ttggtcgcgc cgctcgtcag cacccgccgg cgtttcgagc
     3661 cattcacgca gccacgccgc gccgttcgct tccttggcga ggcgtgccaa gtccgtcttc
     3721 aaatccgcgg ccggggtctt ggcgagatcg gctcctcgct tcaggagctc ggcgtggaag
     3781 agccgcgttg cggtggcatt cttcagatcc gagaattgac cccatagcac acaagttttg
     3841 tgcttggccc gggtcaacgc gacatacgcg acgcgcagct cttccgcgag ctgctcgtgc
     3901 ttggcgcgtt cttggctctg cttgcggcgc tcggaggcga agacctcgag atcgaggaag
     3961 gctctacctt gttcgtcgtg aaagggtaga gggccgggtc tgtcgcgagg gccgttgctg
     4021 caccagagat atgggcagta cacgacgggg aattccaagc ctttggattt gtggaccgtc
     4081 agcacgcgca cggccagctc gtcgctctcc agtcggatct ccgactcgtc tccgcgcggg
     4141 cgatctcggc gctgttgggc aaaccagcgt accagggcgg cgggtccgag ctgttgcgtg
     4201 acgctcgccc gatgcagcaa ctcgccgagg tgcagaagat tgaccaaaca acgttctccc
     4261 cctgcgtcgt aagccaagct ctctcgagtt tggatttcgt cgaacaatgg ccggaacatg
     4321 cggacgaagc cgtgctctgt ccagagcttg tggaagcgtc gaaacggctc cgcgtattcg
     4381 tagaatccgg agtcgttggt atcgagctcg gcgatcacct ccgccgagac cccgaatagc
     4441 cgcgtggaga gcgcgcgacg cagctcgtag ttgttgctcg gatccagcgc cgcccccaag
     4501 acgagggcga gctcacgggc ttcgtcggag tcgaacacgc tcttgtcgcc gatcacgacc
     4561 gcgtgaatac ccacgttgcg gagcgcgtcc tgcacgatga agcattgatc gttggtgcgg
     4621 gtcagaaccg cgatgtcgag ctccgtgagc tcgcgtgagt tcgcggtcgc gcgtcgcgcc
     4681 aggagtgccg cgatgtctga agccacgatc tgggccatgc ggcgcaaggt cagcggccgt
     4741 ggctctgatt tgcctccggg ggccgcttcg acccaaagga actccaatcc cgcccgatca
     4801 tgaggctcgg gccccgagag ggcgtcggcc gcgccggggc gggcctgtac ggggcggtaa
     4861 gagatatgcg gattcgcaaa agcatcctcc gcacaaaata cgatatttac cgcgcggacc
     4921 atgcttgggt ccgagcgaaa gttggtgtcg agggtgaagc gtctcgcgtt ctcgctcgcg
     4981 cgcagatagg taaagacatc cgcgccccgg aatgcgtaga tggattgttt ggggtcgccg
     5041 atcaggaacc aaggcaagcc ggtgtcacgg tagattgtct cgaagatgcc gaattgaatc
     5101 gggtcggtgt cttgaaactc gtcgatcaag agcgccttga agcggtcgcg aagtgagccg
     5161 gcgagcagcg ggcctcgctc ggaatgtagc gcctcgtgaa gccgctgcag caagtcctcg
     5221 aagcccagca ccgagcggcg atctttgcgg atgcgaaatt cacggcgcgc gtagtgcgcc
     5281 aagcgatgct ggagctcgtg accgcgttcg aacgccaacc tcgcaaagcg ctcgaaggcg
     5341 aagaagaact cgggcagggg aaccttcttt ttcagcgcct cccccaaggt ctccgggaac
     5401 aaggcgtcgg aatcttgcgg ggcaaatact tgacgctctt tgaagacgcg gtcgagcgtg
     5461 cgacacagcc ggtcccgccg ctgcgagctc agaccggaaa ttcggctcac gtacacgtac
     5521 tctcccaaga agcctggtag atcgatggag cggtacgcaa gctccgcggc ggcgaaggct
     5581 tcctccagcg cttgctccgt gacaacccgg gcttccggaa ccagagtgac gtttggattc
     5641 tgttggacgt agcgcaagag ctctgcgagg cgcttgggcg taagccccaa ctcgtcgagc
     5701 tgtcgcgcga actccagcgg tcgagatgcg acgtgagtat gccagaagtc gtacaaaata
     5761 tcgtcgcgca gctcgtccaa atcgggcact agttctgcgt cgaagccgag tcggactccc
     5821 accgcgtact gcttgagcac gcgttgacaa aagccatgga tggtcgaaat gggagtctgg
     5881 tcgaggtcgc ctcggcagcg ttcgagatga cgcaggtcgg agctgcgctc gcgtatccgc
     5941 cgttgcaaca gctctttgag gaatgcgtct tccggctcgg cttcgcgggg gtcgcgtttc
     6001 aaccagtgaa gcgcgtcctc gacgcgtgcg cgcacacgct ccccgagctc gacggtggcg
     6061 gcctcggtga acgtgacgac tccgacctca ctcgggtcga ggcgccgctc caagagcagc
     6121 ctcaggaaca agctcgtgat cgtgtaagtc ttgccggtgc cggcgctggc ctcgaccaac
     6181 gtgccaccgc cccgcgcgac cgccagctcg acactcaacg ggtagagcgg agccatatcc
     6241 gtcttcatga ttcgtgagct ccgaggaggt cctggaagca atcgaagaac gccagcgagg
     6301 ctgcgtggaa gtgcgtctct tcgagtgatt tgcagccttg atcgtaggcg ctatcgaacg
     6361 gtggcatgag cccgcggaag atgcgttcga cgtgctgatc gacgatgccg gttcgattcg
     6421 attcggcgta ggccttgatc gctttgtcca gcgcctcttc cggcttcttg ccgcgcaaag
     6481 cgaggaagta ggcctggctc ggtaccggca aaaatggcag cggcaaggcc tgacctttgg
     6541 cgaaccaatg aatcaaggtt tcgaggcgtg ctcgggccag gtcggggccg agcggattga
     6601 gtcgaatcgt gtcgacggat tgttgttttt gacctctgag caccgccaca ctcgggctcg
     6661 gagcccccgc cgcacacatt gccaggtgct tcacaccgag cttgacgagc tgcttgggac
     6721 cgctgctcga atagccgtgt tcgacgcgcg cagcttggaa gcgagcgtcg atactgccgg
     6781 tgagcgtgac ccccgccaag gggagctgca aacgcagtac cggctctgcc gggccgccac
     6841 gtgctttctg cgtggcgagc gcgatgcgta gg
//
LOCUS       DASTKU010000994         9970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1536352, whole genome shotgun sequence.
ACCESSION   DASTKU010000994 DASTKU010000000
VERSION     DASTKU010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536352"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VFQ61_35320"
     CDS             <1..668
                     /locus_tag="VFQ61_35320"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HET9959833.1"
                     /translation="LDEEPVPAWSTFYERLLAEPDRPHRIDFESSRDGRARTGTIQLR
                     REEFTDEHGQSFEKYVLPLQHWVPVAPEQRVSHPTPLRYALEKAVEETVDVTRFVLVG
                     IVRVIQGRVSLKSLSGPITIFQVAGEEGRKGTDYFLWAMALISINLGLFNLLPIPVLD
                     GGHLLFFLIEGVLRRPLPLRAREVLHIVGMAILLGLMLLAFKNDVERRWDGMLGVAKN
                     QTQ"
     gene            689..1990
                     /locus_tag="VFQ61_35325"
     CDS             689..1990
                     /locus_tag="VFQ61_35325"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET9959834.1"
                     /translation="MNDTPSTARVPNARALAARVLSRVITDRAFAASVLDTEIARHPQ
                     LEARDRALLTELVYGVLRTRGALKARLLALAPRGIRDSKTELHLWVAAYQVLMLDRVP
                     AFAAVNAAVDLIRAERGPQMAGFANAVLRRLAQSTERPSIEQALRETVPSWLLERLSQ
                     AVGEEEALGALGAGGTEPCVGVRLVSGHAVPEWLADEPAGVMPRTRRLQRKGDPRSRA
                     GYAEGSFVVQEEGAQLVGTLLGARAGERVLDACAGRGQKSSLLAEQVGPSGAVFATDV
                     FPQKLEALCEEFTRLGLPAPETRALDWTVGAGDVPQDFDRVLVDAPCTGTGTLRHRPE
                     ILDRLGPQDPARLGELATRILRGAASRAKPGGRVVFAVCSLLREECEDTVARVADLLD
                     PVPFDAPELGSWLPSGQTALRLLPLKHGTDGYFVASLRRKP"
     gene            2078..2968
                     /gene="dapA"
                     /locus_tag="VFQ61_35330"
     CDS             2078..2968
                     /gene="dapA"
                     /locus_tag="VFQ61_35330"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET9959835.1"
                     /translation="MTALPPGAYTALVTPFTPDGSSIDWAAFENLVASQIEGHIAGVV
                     PCGTTGESATLSEAEQRDLIRRTVELARGRTKVVAGTGSNNTKKSIEASRAAVEAGVD
                     GVMLVNPYYTKPSQEGLYQHFVSIARSVPCPVMLYNIPGRCSVELAVPTLLRILDACP
                     NVVALKDASGGVSYCQDLLQQAYGRIRVFSGDDPLTVPMMSVGAEGVISVTSNLYPAQ
                     VSDLVSEMLAQRWQEAQRKDRRLLPIHRALFSEPSPQPIKAALSLKGRMNAAVRLPLV
                     VASDACKERLSAVMSAFEAA"
     gene            2965..3762
                     /gene="dapB"
                     /locus_tag="VFQ61_35335"
     CDS             2965..3762
                     /gene="dapB"
                     /locus_tag="VFQ61_35335"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET9959836.1"
                     /translation="MKLAIFGATGRMGLAIVRLSQEDSSIQIVGGICGPDDKSAGRDL
                     GDLAGTGTLGVVATPDLASGLLGADVVIDFSNFKAAPALFATAARQRVAIVSGTTNLD
                     EPGMRALDKAAEHVPVLWAANMSLGVQIMAELVEYAVRRLGAEFDVEIMEVHHKRKVD
                     APSGTAKRLAQAARAARTELTELSSRVGDVGARTPSEIGIFGLRGGDVIGDHTVFLFG
                     PGERIELTHRASNRDLFARGALRAASFLAGKAPGRYTIKDVLTPERA"
     gene            complement(3743..4960)
                     /gene="lgt"
                     /locus_tag="VFQ61_35340"
     CDS             complement(3743..4960)
                     /gene="lgt"
                     /locus_tag="VFQ61_35340"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET9959837.1"
                     /translation="MLPTVTWNVDPVFLRLPREGVAIALGAFGALSLVYGLAKKAKDQ
                     VFSALFFALLAVVVGKYMPENYGIRYYSLIFVGVFLGGHALLRWQIRRAGGPSEDAND
                     FILYGVLGVLLGARLGHVLFYDLDKAIENPAWVLQIWTGGLASHGAVLGLIIAMFLFT
                     KRRGISFLEGSDRFAYSAALGATLVRLGNLLNSEIVGKQTDGTWGFRFPRFEADPPLR
                     HPSQIYEVALGLTVLGALYLFDKKLGEEKRPRGAMISGFFLLYFVGRFVVEFFKEFQP
                     GESTETGIRMGQILSLPGILLGVYGLYWAFSRKIPAQWPHAAPPPELDQDEDEDDDDE
                     DDEEDSSDEDDDAADEEDEEQPSARSAPKKPADANPRASKKGSRPATENEDLDDDVAD
                     EFDAEGRLKRVRE"
     gene            complement(5181..5978)
                     /locus_tag="VFQ61_35345"
     CDS             complement(5181..5978)
                     /locus_tag="VFQ61_35345"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9959838.1"
                     /translation="MRKSVRWGGAALLGLLTLGLTGFALVVRSKRSQADPVERVENGV
                     FRARNFLSDIYAARVGGHVLLFDAGLDPEGHAADALLSTLHARREDVSHVFLTHGHAD
                     HVAAAGLFPKAHIYVGAGDAAVLEHPELAEPRTPRWFGALMGRVSVHADRPLSGPTTV
                     EVGEGRVVRAIPFPGHTPGSFVYWFERVLFTGDSVGLDRRVPPDGVLTAAQPRHSVDV
                     AGNHESIRALPKWLAKDEVEVLCTGHMGCTRAQATRPLLDRLLAETK"
     gene            6395..>9970
                     /locus_tag="VFQ61_35350"
     CDS             6395..>9970
                     /locus_tag="VFQ61_35350"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MupA/Atu3671 family FMN-dependent
                     luciferase-like monooxygenase"
                     /protein_id="HET9959839.1"
                     /translation="MHDPSIETSHALTPIQQGMLLHFVQAPESGVDVEQMVARFSMPL
                     DRHLLERALRAVVDAHPALRSCFRWEGVAEPQQVVLAHVDARIEERRFEVGAAEEREA
                     QFDAWLLEDRRQGLELTAAPLFRAWLVQFTEHDTRLVWTFPHILLDGGSFGKVITDWF
                     EAYVALREGRTPEPKASRPYADHSAWLRAEIEAKRGAAAQHFAGLFAGFSAKNEISEP
                     RPPRAPARRRRIYDHVGTRLSAELTARLRDLGAERGCSLNNLVQAAWALVVSDFSGNE
                     DAVFGVVRGCRGTGLPEARDMVGLFINTLPLRVNCGTQQELGEFIVGVRTQHRALRDF
                     EHTPLSDVARAAQVDSAASLFDSIIVFNERRVGAELRERGGIFASAEIEFIEQTNFAV
                     TLFGYAEAELELAISYDPTRLGAARASAMLERLTTALQAFSAGLGRTLSELDRVPPSE
                     LARLASSRGASVPLSEQASIHEAFAAQVQRTPHAEALVFRGQSLTYAELNRRANQVAR
                     ELATLGVTRDVLVGVHVDRSLEMVIALLGTLKASGAYVPLDPTYPKERLAMMLEDARP
                     KVVLSTSRLSRELPAHAGRTLAIDELLSDGAGAENPPPNSADRDALSYVIFTSGSTGR
                     PKGVMVEHGNVLNFFAGMDERVGREPGVWLAVTSISFDISVLELLWTLTRGFKVIVQE
                     EGDKAHLSRQRAAQKGMQFSLFYFASDAGTKTEPGRGVYKLLLEGARFADQNGFTAVW
                     TPERHFHAFGGLYPNPALTSAAVAAVTERVQLRAGSIVLPLQNPIRVVEDWSFIDNLS
                     QGRVGLSFASGWHANDFALRPENYERRRELMFEYIETIKRLWRGESITVKNGVGADLE
                     IRTLPRPVQKAPPIWITAAGSVDTFKAAGSLGANLLTNMLGQSVTDLREKIAAYRAAR
                     AAAGFEGAGHVTLMLHTFVGTDVAEVKSLVHRPFIDYLRTSTDLVRRAKWYFPAFARP
                     GQAAPTETPDTLDAEDEAALLEHAFERYFETHGLFGTPESCLPKLERLSEIGIDEIAC
                     LIDFGVDEEQVLNSLPHLARLHQLANANVSAAAANATPSAYAEIASQVTRHEVTHLQC
                     TPSLARMLLESSEAPSTFGPLRALMLGGEALPPSLADRVLSLMTDGRLLNMYGPTETT
                     VWSTTARIQHGEPITIGTPIANTSIYILDGDAKPRPFGVPGELCIG"
BASE COUNT         1710 a         3295 c         3252 g         1713 t
ORIGIN      
        1 gcttggatga ggagcccgta ccggcctggt ccaccttcta tgagcggctg ctggccgagc
       61 ccgatcgccc gcaccgcatc gatttcgagt cttctcgcga cgggcgcgcg cgcacgggga
      121 cgattcagct gcggcgcgag gagtttaccg acgagcacgg tcagtccttc gaaaagtacg
      181 tgctccccct ccagcattgg gtgccggtcg cgcccgagca gcgtgtctcg cacccgaccc
      241 cgcttcgcta cgcgctagag aaggcggtcg aagagacggt ggacgtgacc cgcttcgtgc
      301 tcgtcgggat cgtgcgggtc attcaggggc gggtcagcct caagtctctg tccggcccga
      361 tcacgatctt ccaggtcgcg ggggaggagg ggcgcaaggg caccgactac ttcctctggg
      421 ccatggcgct gatcagtatc aacctgggcc tcttcaatct gctcccgatc ccggtgctgg
      481 atggcggaca cttgttgttc ttcttgatcg aaggcgtctt gcggcgaccg ctcccgctcc
      541 gtgcacgcga ggtgctgcac atcgtgggca tggcgatttt gctgggcctg atgttgctcg
      601 ccttcaagaa cgacgtagaa cgccgctggg acggcatgtt gggcgtcgcc aaaaatcaaa
      661 cccagtaaat gaaacccagt agagctcctt gaacgacact ccttcgactg ctcgggttcc
      721 gaacgcgcgc gctttggcgg cccgcgtgct ttcgcgtgtg atcacggatc gggctttcgc
      781 ggcctcggtg ttggacaccg agatcgcgcg tcacccgcag ctcgaggcgc gcgatcgggc
      841 gctcttgacc gagctggttt acggcgtttt gcgcacgcgg ggggcgctga aggctaggct
      901 gctggcgctc gcgcctcgcg gcatccgtga cagcaaaacc gagctccatc tgtgggtcgc
      961 ggcgtaccag gtgttgatgc tcgatcgcgt gccggcgttt gccgcggtca acgccgcggt
     1021 ggacctgatt cgcgccgagc gagggccgca aatggcaggc tttgccaacg cggtattgcg
     1081 gcgtctggcc caatcgacgg agcgcccgag catcgagcag gccctccgcg agaccgtgcc
     1141 cagttggctt ttggagcgtc tcagccaagc cgtcggggaa gaggaggcgc tcggcgcgct
     1201 cggggcgggc ggcacggaac cttgcgtcgg ggttcgcctc gtgagcgggc acgccgtccc
     1261 cgaatggctc gctgacgagc ccgcgggagt aatgcctcgc acgcgccgct tgcagcgcaa
     1321 gggagatccg cgcagccgtg ctggttacgc cgaaggaagc ttcgtcgttc aagaggaggg
     1381 cgcgcaactc gtcggcacgc tgctgggcgc gcgggcgggc gagcgggtgc tcgatgcctg
     1441 cgcggggcga ggccagaagt cgagcttgct cgcggagcaa gtggggccaa gcggcgcggt
     1501 gttcgccacc gacgtgttcc cccagaagct cgaagccctc tgcgaggagt ttacacgcct
     1561 cggcttacca gctcccgaaa cgcgagccct ggattggacg gtgggggccg gcgacgtgcc
     1621 gcaggatttc gaccgggtgc tcgtggacgc cccctgcacc ggaaccggta cgctgcgcca
     1681 ccgtccagag atcctcgacc gcttgggccc gcaagatccc gcccgcctcg gtgaactggc
     1741 tacccgtatt ctgcgcggcg cggcaagtcg cgcgaagccc ggagggaggg tcgtgttcgc
     1801 ggtctgcagc ctgctccgcg aggagtgtga agacaccgtg gctcgcgtcg cggatctgct
     1861 cgaccccgtc ccgttcgatg cgccggagct cggctcgtgg ctacccagcg ggcagacggc
     1921 gctgcggctg ttgccgttga aacacggtac agacggctac ttcgtcgcca gcttgcgtcg
     1981 caaaccctga tttatactgc ctagggcccg ggccagccgc agcttgagac gcggctggcg
     2041 cttgtgtccg agcctggaac gcggcaaaga cagcgccatg acagcgcttc ccccgggcgc
     2101 ctacacggcg ctcgtgaccc ccttcacccc cgatggctcc agcatcgact gggccgcatt
     2161 cgagaacctg gttgcgtcgc aaatcgaagg ccacatcgcc ggcgtcgtgc cgtgcgggac
     2221 tactggcgag agcgccacgc tgagcgaggc ggagcagcgc gacttgattc gccgcaccgt
     2281 agagctcgcg cgggggcgca ccaaggtcgt ggcgggtacg ggctcgaaca acaccaagaa
     2341 gtcgatcgaa gcatcgcgcg ccgccgtgga agccggcgtg gacggcgtga tgctggtgaa
     2401 tccgtactat acgaagccgt cgcaagaagg gctctatcag cacttcgtca gcatcgcgcg
     2461 atcggtgccc tgcccggtga tgctgtacaa cattcctggg cgttgctcgg tcgagctcgc
     2521 agtgccgacg ctgctgcgta tcctggacgc atgccccaac gtcgtggcgc tgaaggacgc
     2581 cagcggcggc gtgagctact gccaggattt gttgcagcaa gcctacggac gcatccgcgt
     2641 cttctccggg gacgatccgc tcacggtgcc gatgatgagc gtgggcgccg agggcgtcat
     2701 cagcgtcacg agcaacctct accccgcgca ggtcagcgat ctcgtgtccg agatgttggc
     2761 gcagcgctgg caagaagcgc agcgcaaaga tcgccgtttg ctgccgatcc atcgcgccct
     2821 attcagcgaa ccgagcccgc aacccatcaa ggctgctctg tccctcaagg ggcgtatgaa
     2881 cgcggcggtg cgcttgccgc tagtggtggc gtcggatgcc tgcaaagagc ggctcagcgc
     2941 ggtgatgtcc gccttcgagg cagcatgaag ctcgccattt tcggggccac cgggcgcatg
     3001 ggcttggcca tcgtgcggct gagccaggaa gactcttcga tccaaatcgt gggtgggatc
     3061 tgcggccccg acgacaagtc cgccgggcgt gatttgggcg atctcgccgg cacgggcacg
     3121 ctcggcgtgg tggccactcc cgatctcgcg tcgggcctac tcggcgccga cgtggtgatc
     3181 gacttctcga acttcaaggc ggcccccgcg ctgtttgcca cggccgcgcg ccagcgcgtc
     3241 gcgatcgtga gcggcaccac gaacctcgac gagccgggca tgcgcgcgct cgataaagcg
     3301 gccgagcacg tcccggtgtt gtgggccgcc aacatgagcc tgggcgtgca aatcatggcg
     3361 gagctcgtgg agtacgccgt gcgccgattg ggcgctgagt tcgacgtcga aattatggaa
     3421 gtgcaccaca agcgcaaggt ggacgccccg agcggcacgg ccaagcgcct ggctcaagcc
     3481 gcgcgcgcgg cccgcaccga gttgaccgag ctgagctcgc gcgtgggcga cgttggcgcg
     3541 cgcacaccat cggagattgg catctttggg ctacgcggcg gcgacgtgat cggcgatcac
     3601 accgtattct tgttcggccc cggcgagcgc atcgagctca ctcaccgggc ctcgaaccgc
     3661 gacttgttcg cgcgtggggc cctgcgcgcg gcgagcttcc tcgccggcaa ggcccccggt
     3721 cgttacacga tcaaggacgt gctcactccc gaacgcgctt gagacggcct tcggcgtcga
     3781 attcgtccgc gacgtcgtcg tccaaatctt cgttttcagt cgccggacgc gagcccttct
     3841 tggatgcgcg agggttcgcg tccgccggct ttttcggggc ggatcgagca ctcggctgct
     3901 cctcgtcttc ttcgtcagca gcgtcgtcgt cttcgtcgct cgaatcctct tcgtcgtcct
     3961 cgtcgtcgtc gtcctcgtct tcgtcctgat cgagttcggg cgggggcgcc gcgtgcggcc
     4021 attgcgcggg gatcttgcgg gagaacgccc aatacagccc ataaacaccg agtaggatac
     4081 cgggcagact caatatctgt cccatgcgaa tccccgtctc ggtcgattca cccggttgga
     4141 attccttgaa gaactccacc acgaagcgcc ccacgaagta gagcaggaag aagccggaga
     4201 tcatggcccc gcgaggccgc ttctcttccc cgagcttctt gtcgaacaga tacagggctc
     4261 cgagcaccgt caggcccagg gcaacctcgt aaatttggct cgggtgacgc agcggtggat
     4321 ccgcttcgaa gcgcgggaaa cgaaagcccc aagtgccgtc cgtctgcttg cccacgattt
     4381 cggagttcaa caggttgcct agacgaacca gcgtggcgcc cagcgccgcc gagtacgcga
     4441 accgatccga gccctccagg aacgagatcc cgcgacgctt ggtgaacaag aacatcgcga
     4501 taatcaagcc aagcacagcg ccgtggctgg cgaggccgcc cgtccagatc tgcagtaccc
     4561 aagcggggtt ctcgatcgct ttgtcgagat catagaacaa cacgtgcccg agccgagcgc
     4621 caagcagcac gcccagcacg ccgtacagaa taaagtcgtt ggcgtcctcc gaaggccctc
     4681 cggcgcgccg gatttgccat ctcagcagcg cgtgaccgcc caagaacacg cccacgaaga
     4741 tcagcgagta gtaacgaatg ccgtagttct ccggcatgta cttgcccacc accaccgcga
     4801 gcagggcgaa gaacagcgca gagaaaactt gatctttcgc cttcttcgcg agcccataaa
     4861 ccaacgacag cgcgccgaac gcacccagtg cgatggccac accttcgcgc ggcaatcgga
     4921 ggaagactgg atcgacattc caggtaacgg tgggcagcat ggatcctctt tcgaacggcc
     4981 ccgcgcgcga gcggaaagtc ccgcgggcga ggagggcagg tcagtagctc aggccggcgt
     5041 cagaagccag acactggggt ttgcagtcga aacatcgctg gggcggcgag cctggtagcc
     5101 gagtttaagg gcgtggtcac gccccgcggc ctggcgggcg gccggcgtag ccaccctctc
     5161 tcggtcggcg aagaacatca ttacttcgtc tcggccaaca atcggtccag caacggccgc
     5221 gtggcttgcg ctcgggtgca ccccatatgg cccgtgcaca gcacctccac ctcgtctttt
     5281 gcgagccatt tcggcagcgc gcggatgctt tcgtggttgc cggccacatc cacgctatgt
     5341 cgcggctgag ccgcggtgag taccccatcc ggtggaaccc gcctgtccag cccaaccgag
     5401 tctcccgtga acagcacgcg ctcgaaccaa taaacgaacg agcccggagt atgcccggga
     5461 aaaggaatcg cgcgcacgac gcggccctca cccacctcca ccgtcgtggg accggagagc
     5521 ggccgatcgg cgtgcaccga aacacgcccc atcagcgccc caaaccagcg cggtgtccgc
     5581 ggctccgcta actcggggtg ctcgagcacg gctgcgtccc ccgcgcccac gtagatatgc
     5641 gccttgggaa atagaccggc tgcagcaacg tgatcggcat gcccgtgcgt caggaatacg
     5701 tgactcacgt cttcgcgccg cgcgtgcagc gtcgacaaca gcgcatccgc ggcatgccct
     5761 tccggatcca gcccggcgtc gaacagcagc acgtgcccgc ccacgcgagc ggcataaata
     5821 tccgagagaa aattgcgcgc ccggaagacg ccgttttcga cccgctcgac gggatccgct
     5881 tgcgaccgtt tgcttctcac cacgagcgcg aacccggtga gccccaacgt cagcagtccg
     5941 agtagcgccg cccctcccca gcgcaccgat ttccgcatgt ggcaccaacc cagagccgcg
     6001 gtcacctagt cccgcgcggt aagcagatgt cttatgggcc tcgggatcac gtgcgcgcct
     6061 gcgcattctg ggggacccgc ggcgcagacc tcggagccct aagccgctcg cggcaccgcg
     6121 cgggccccgc ctcgtaaaga ccccgcgagg gcgtcggttc cgtcctgaag taacgcacac
     6181 aatccctctt tcgacggcga gcggccccga aagggcgcca aaccgcactg catcccaccc
     6241 cagatggcgc gccgttcatg ggaaattcgg cgcaaggctt ggcctcgaaa ccttcgcgcg
     6301 gctcgccacg cgcgagccac cccacggcac cgctcgctat ggcttctggc cctagaggac
     6361 ttgtggcaaa acggggattc cgaacaggta cgccatgcac gatcccagca tcgagaccag
     6421 ccacgctctg acgcccattc agcagggcat gttgctccac ttcgtgcagg cccccgagag
     6481 cggggtggac gtggagcaaa tggtggcaag gttttcgatg ccgctcgacc ggcacttgct
     6541 cgagcgggcg ctgcgcgcgg tggtggacgc gcatcccgcg ctgcgttcgt gtttccgttg
     6601 ggagggcgtg gccgagccgc agcaagtggt gctcgcacac gtcgatgcgc gcatcgaaga
     6661 acggcgcttc gaggtcggcg cggccgagga gcgggaggct cagttcgacg cctggttgct
     6721 cgaagatcgc cgccaaggcc tcgagctgac cgcggcgccg ctgttccgcg cttggctggt
     6781 tcagttcacc gaacacgaca cgcggctggt ctggactttc ccccacatcc tcctcgatgg
     6841 cggcagcttc ggcaaagtca tcactgattg gttcgaggcc tacgtggcct tgcgcgaggg
     6901 ccgcacgccg gagcccaagg cgagccgccc ctacgccgat cacagcgcct ggctgcgcgc
     6961 ggaaatcgag gccaaacgcg gcgcggcggc ccagcatttc gccggcctgt tcgcgggctt
     7021 ctcggcgaag aacgagattt ccgagccccg cccgccgcgc gcccctgcgc gtcgccgtcg
     7081 aatctacgat cacgtcggca cgcgcctttc cgcggagctt acggcgcgcc tacgtgacct
     7141 cggcgcggaa cggggctgct cgctgaataa cttggtgcag gccgcctggg cgttggtggt
     7201 gagcgatttc tcgggcaacg aagacgcggt attcggcgtc gtccgcggct gccgtggcac
     7261 cgggctgccc gaagcgcggg acatggtggg cctgttcatc aacacgctcc cgttgcgggt
     7321 gaattgcggg actcagcaag agctcggcga gttcatcgtc ggcgtgcgga cccaacaccg
     7381 cgctttgcgg gacttcgagc acactccact ttcggacgtg gcccgcgccg cgcaggtcga
     7441 cagcgccgcg agcctgttcg acagcatcat cgtgttcaac gagcgccgcg tgggagccga
     7501 gctgcgcgaa agagggggga tcttcgcctc cgcggagatc gagttcatcg agcagaccaa
     7561 cttcgccgtc accctgtttg gttatgcgga ggccgagctc gagctcgcga tctcctacga
     7621 tccaacgcgg cttggggcgg cgcgcgcgtc cgccatgttg gagcggttga ccacggccct
     7681 gcaggctttc agcgcggggc tgggacgcac gctctcggag ctcgaccgag ttccgccctc
     7741 cgaactcgcc cgcctggcgt cctcgagagg cgcgagcgtg ccgctcagcg agcaggcctc
     7801 gatccacgag gcctttgcgg cacaagtgca gcgtaccccg cacgccgaag ccttggtgtt
     7861 tcgaggtcaa agcctcacgt atgccgagtt gaatcggcgc gccaaccagg tggcgcggga
     7921 gctcgcgacg ctcggcgtga cccgagacgt gctggtcggg gtgcacgtgg accgctcgct
     7981 cgagatggtg attgccttac tcggcacgct caaggcgagc ggcgcctacg taccgctcga
     8041 cccgacttac ccgaaagagc gcctcgcgat gatgctcgaa gacgcacgac ccaaggtcgt
     8101 gttgagcacc tcgcgacttt cgcgtgagct gcctgctcat gcagggcgta cgttggcgat
     8161 cgacgagctc ttgagtgacg gcgcgggtgc ggagaatcct cctcccaaca gcgcggatcg
     8221 cgacgctctg agctacgtaa tcttcacctc cggctccacc ggcaggccca agggggtgat
     8281 ggtcgagcac ggtaacgtgc tcaacttctt cgcgggtatg gatgaacgcg tcgggcgcga
     8341 gcccggagtg tggctcgcgg tcacatccat ctcgttcgac atctcggtgc tggagctgct
     8401 gtggacgctg acacgtggct tcaaggtaat agttcaagaa gagggcgaca aagcgcattt
     8461 gtctcggcag cgcgcggccc aaaagggcat gcagttcagc ctcttctatt tcgcttcgga
     8521 cgcgggcacg aagaccgagc ccggacgcgg cgtgtacaag ctgctgctcg agggggcgcg
     8581 cttcgcggat caaaacgggt ttaccgcggt gtggacgccc gaacgtcact tccacgcctt
     8641 cgggggcctg tacccgaatc cggcgttgac gtcggcggcc gtggcggccg tgaccgagcg
     8701 cgtgcagctc cgcgccggaa gcatcgtgtt gccgctccaa aatccaatcc gcgtggtcga
     8761 ggactggagc ttcatcgata acctgtctca gggccgcgtg ggcttgtcgt tcgcttcggg
     8821 ctggcacgcc aacgatttcg cgttgcgacc ggagaactac gagcgtcgac gcgagttgat
     8881 gttcgaatac atcgagacca tcaagcgact ttggcgcggt gagtccatca ccgtaaagaa
     8941 cggcgtcggc gcggatctcg agattcgcac cttgccgcgt ccggtacaga aggcccctcc
     9001 catctggatc acggccgccg gcagcgtgga caccttcaaa gctgcgggtt cgctgggcgc
     9061 gaacctgctc accaacatgc tcgggcagag cgtgaccgac ctgcgtgaga agatcgcggc
     9121 gtaccgcgcc gcccgtgcgg cggcaggctt cgagggggcc ggtcatgtca ccttgatgct
     9181 gcacaccttc gtcggcaccg acgtggcgga ggtgaagagc ttggtgcacc ggccattcat
     9241 cgactacctg cgcacttcga cggatctggt ccgacgcgca aaatggtact tccccgcgtt
     9301 tgcccgtccg gggcaagccg cgcccaccga gactcccgat acgctcgacg ccgaagacga
     9361 ggccgcgctc ttggagcacg ccttcgagcg ctacttcgag acccacggct tgttcggcac
     9421 gcccgagagc tgccttccca agttggagag actctcggag attggcatcg acgagatcgc
     9481 ttgtttgatc gacttcggag tggatgaaga gcaggtgctg aacagcctgc cgcatttggc
     9541 gcgattgcat cagctcgcga atgcgaacgt aagtgccgcc gccgcgaacg cgacccccag
     9601 tgcgtacgcg gaaattgctt cgcaagtcac tcgccacgag gtgacgcatc tgcagtgcac
     9661 gccttcactc gcgcgcatgt tgctcgaaag cagcgaggcc ccgagcacgt tcggtccgct
     9721 ccgtgcgctg atgctcgggg gtgaggcgtt gcccccgtca ttggcggatc gcgtcttgtc
     9781 gttgatgacg gacgggcgct tactcaacat gtacggcccg acggagacca cggtgtggtc
     9841 caccaccgcc cgcatccaac acggcgaacc gatcacgatt gggaccccga tcgcaaacac
     9901 ctctatttat atcctcgatg gcgacgcgaa gcctcgaccg ttcggggtgc cgggtgagct
     9961 ctgcatcggg
//
LOCUS       DASTKU010000995         5160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1312344, whole genome shotgun sequence.
ACCESSION   DASTKU010000995 DASTKU010000000
VERSION     DASTKU010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312344"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="VFQ61_35355"
     CDS             complement(<1..1587)
                     /locus_tag="VFQ61_35355"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HET9959840.1"
                     /translation="MRFGHFDDKNREYVIETPKTPYPWINYLGNENFFGLVSHQGGGY
                     CFYRDARLRRLTRYRYNNVPVDTGGRYYYVFDRGDYWTPTFMPTKKPLEKFECRHGLG
                     YTRITGSRNGIETEVLYFVPQGETCELHKVTLRNHSKEKRSLKLFSFVEFCLWNAFDD
                     MTNFQRNFSTGEVEVDGSTIYHKTEYRERRNHYAVYHANAPVAGFDTDREAFVGTYNG
                     FGEPDAVVEGRPRNSIASGWHPIGSHCIEVELEPGAEKTVVFVLGYVENPVSEKWEKP
                     GIVNKRRAHELIERYSTAAAVDAALERLKSYWQELLSGYQVSSSDEKLDRMVNIWNQY
                     QCMVTFNMSRSASYFESGIGRGMGFRDSNQDLLGFVHLVPERARERIIDIASTQFADG
                     SAYHQYQPLTKRGNNDIGSGFNDDPLWLILGVAAYIKETGDYAILDEIVPFDNDPNNT
                     ATLLEHCKRSFDHVLNNLGPHGLPLIGRADWNDCLNLNCFSETPDESYQTTGNRTGRT
                     AESIFIAGMFVYIGPEYAALC"
     gene            complement(1911..2645)
                     /locus_tag="VFQ61_35360"
     CDS             complement(1911..2645)
                     /locus_tag="VFQ61_35360"
                     /inference="COORDINATES: protein motif:HMM:NF015390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HET9959841.1"
                     /translation="MKHTAYLLKTMVLMACVALPGCRKRIDPAAAETREMPEGRACPA
                     SGMISDNENGANKSNVVDGRGGYWYSFLDEKGSSITPTAGRLGGTFSMTGGGANGTKF
                     AARFEGSLAQGEAGQVYAGAGVNFVDPKGQYDASKYGGISFWAKHGPGSTNKVRLKVP
                     DVATDPDGKMCSACFNDFGIDLVLTPEWQQYVVPFSAMKQIKGWGSPHTSGIDKSKLY
                     AVQFQVGDPGVKFDIWVDEIQFTGCQ"
     gene            complement(2962..4077)
                     /locus_tag="VFQ61_35365"
     CDS             complement(2962..4077)
                     /locus_tag="VFQ61_35365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959842.1"
                     /translation="MRASGFFTGTLFLSLGFLFASSSRAAGARDAEATELQHRAMNTE
                     YLAGKAGAAERTLLKALQRCGKDGCSQQVLAKLHRDLGVVYVAGLGKQAKGRKALARA
                     VSYDRFIQLDPDLTTPELARVFKEVGGGKSEEVVLEEEPTAEAPAAELGEPEATPPEQ
                     VTGHRYWVSLHLQQDMLWHPSTNPACYGASYTCFGSGRVEYTDPIWDGEGNRTAAGFG
                     LASTRLLIGFDRVFAKRWLLGARLGVAFGGAPTPKNGDKFLPLHAELRGTYLFGQSPL
                     ERLGFRPFAALGLGVAEIDGHVSVTYYVDEAGFQAGEAGKLDAWRKTGKLFVTPRVGT
                     FYALSPKSMVSLELGTSIMFPTGGFAPAIAGGFSYGL"
     gene            complement(4263..>5160)
                     /locus_tag="VFQ61_35370"
     CDS             complement(4263..>5160)
                     /locus_tag="VFQ61_35370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959843.1"
                     /translation="GMAAAGDSSDTGGASSGASGTGTRPSSGGVSSGGRSQGGAPNTA
                     GSGGMGSGGAAKGGAPSSGGKSSGGTSSGGVATGGTGGASTGGATTWPPELIDDMEKV
                     DHFILPNDGRNGQWFPWADAEKGATVTPSPTPVMASLPTGEKGTAPGSQHALHFTLFG
                     GDAGSQGALMGVEMIQSTDAGQKKFPYDATKYKGIHFWARAQGEEATVLVRLPLRDTL
                     LAARGACDDTAGKLCNDHYSREVTLSTDWQEYTLLWSSFQQGDWGLPVKPFDPSQLVE
                     VQFMAPATFSAELWVDDISFAR"
BASE COUNT          926 a         1656 c         1572 g         1006 t
ORIGIN      
        1 gcaaagcgcc gcgtactcgg gaccgatgta cacgaacatg cccgcgatga agatcgactc
       61 tgcggtacga ccggtgcggt tgccggtcgt ctgataggac tcgtccggcg tctccgagaa
      121 gcaattcagg ttgaggcagt cgttccagtc ggcgcgaccg atcagcggca acccgtgcgg
      181 cccgaggttg ttgagcacgt gatcgaacga gcgcttgcag tgctcgagca acgtcgccgt
      241 gttgttcggg tcgttgtcga acggcacgat ctcgtccaaa atcgcgtagt cgccggtctc
      301 tttgatgtag gcggcgacgc cgaggatgag ccatagcgga tcatcgttga agccgctgcc
      361 gatgtcgttg ttaccgcgct tggtgagcgg ttggtactgg tggtacgcgc tgccgtcggc
      421 aaactgcgtc gacgcgatgt cgatgatgcg ttcgcgcgcg cgctccggga ccaggtgcac
      481 gaaaccgagc aggtcctggt tcgagtcccg gaagcccata ccgcgcccga tgccagactc
      541 gaagtacgag gccgagcgcg acatgttgaa ggtgaccatg cactggtact gattccagat
      601 attgaccatc cgatcgagct tctcgtcgga ggaggagacc tgataaccag acagcagttc
      661 ctgccagtag ctcttcaagc gctcgagcgc tgcgtccaca gcggccgcgg tcgagtagcg
      721 ttcaatcagc tcatgcgcgc ggcgcttgtt gacgattccc ggcttctccc atttctcgga
      781 cacgggattt tcgacgtagc cgagcacgaa gaccaccgtc ttctcggcgc ccggctccag
      841 ctcgacctcg atgcagtgcg agccgatggg atgccagcca cttgcaatcg aattgcgggg
      901 acgcccttca acgacggcgt cgggctcgcc gaagccgttg taggttccga cgaacgcctc
      961 ccgatccgtg tcgaagccgg ccaccggcgc gttggcgtgg tagacggcgt agtgattgcg
     1021 gcgctcgcga tactccgtct tgtggtaaat cgtcgagccg tcgacttcta cctcgccggt
     1081 gctgaaattg cgctggaagt tcgtcatatc gtcgaacgcg ttccagagac agaactcgac
     1141 gaacgagaac agcttgagcg agcgcttctc cttcgagtgg ttgcgcagcg tgaccttgtg
     1201 cagctcgcag gtctcgccct ggggcacgaa gtagagcacc tcggtctcga tgccgttgcg
     1261 ggaccccgtg atgcgcgtgt agccaaggcc gtgacgacac tcgaacttct cgagtggctt
     1321 cttggtcggc atgaacgtcg gcgtccaata gtcgccgcga tcgaacacgt agtagtagcg
     1381 cccgcccgta tctaccggca cgttgttgta gcggtagcgc gtgaggcgcc gcagccgcgc
     1441 gtcgcgatag aagcagtagc ctccaccttg atgggagacc aacccgaaga agttctcgtt
     1501 acccaggtag ttgatccacg ggtacggagt cttgggggtt tcgatcacgt actcacggtt
     1561 cttgtcgtcg aagtgcccga agcgcatgct gctttactcg ttgaggtggg attctcggaa
     1621 acggcgccat cctagtccgg gttccagaaa acccaaggag catttggcgc agatcgggtg
     1681 gggcacgtca ttcgctgttc aggtgcgccc acccgatcct tcttctttgg aattggccgt
     1741 cgactacttt gggattggcc gtcgactacc gcggagatgc cgtccgccct aaatgcggct
     1801 cagttgcagc ctgcccgagg cggcggcaaa cgcccggccc gccctactgc ccgaaacttt
     1861 ccgggagctg cgctcgaagt cccggctctg ggacgccccc ccggtggtgc tcactggcaa
     1921 ccggtgaact ggatttcatc cacccagatg tcgaacttca cgccgggatc gcccacctga
     1981 aactggacgg cgtagagctt gctcttgtcg attcccgagg tgtgcggaga gccccagcct
     2041 ttgatttgct tcatggccga gaacggtacg acgtattgct gccactcggg cgtcaacacg
     2101 agatcgatac cgaagtcgtt gaagcaggcc gaacacatct tcccatccgg atccgtagcc
     2161 acgtccggca ccttgaggcg caccttgttc gtggacccgg ggccgtgctt cgcccagaac
     2221 gagattccac cgtacttgct ggcgtcgtac tggcccttgg gatcgacgaa gttgacgcca
     2281 gctccggcgt aaacctgccc cgcttcaccc tgcgccaggc ttccctcgaa gcgagctgcg
     2341 aatttggtgc cgttcgcgcc gccacccgtc atggagaacg tgccacccaa acgacccgcg
     2401 gtgggggtga tggacgagcc cttctcgtcc aagaacgagt accagtaccc gccgcggcca
     2461 tcgaccacgt tgctcttgtt cgcgccgttc tcgttgtcgg agatcatgcc gctcgccggg
     2521 caagcgcgcc cctctggcat ttcacgcgtc tcggccgcgg ccggatcgat gcgcttgcgg
     2581 cagccgggca gtgcgacgca cgccatcaga accatcgtct ttaacagata agcagtgtgt
     2641 ttcatggatt ctcctcgagg ccacatcgaa gcgtggcgca cgacccgcac cgctggggcg
     2701 aagggtgccg cggtgaatcg ggtgccgtgc atagagtgcg gctatcgggc ccgggatttc
     2761 gcgcgcttca tggggcgaag tcaactcgaa tcgatgcgca aatccgctct ggacctcggc
     2821 ggtctatctc tgcctctgcg gtttggcgct gccggtttgg tgctgccggt ttggtgctgc
     2881 cggctctctg tggcccaact gattatgtct tgctgcttcc gaacggatcg atgaatcgcc
     2941 acggcgcacg attcatcgat ctcagagacc gtatgagaat cccccggcaa tggcgggagc
     3001 aaagccccca gtggggaaca tgatcgaagt tccgagttcg agactcacca tcgattttgg
     3061 agagagggca tagaacgttc ctacgcgggg cgtcacgaac agcttgccgg tcttcctcca
     3121 tgcatcgagt tttcctgctt ctcctgcctg aaaacccgcc tcgtcgacat agtacgtgac
     3181 cgagacgtgc ccatcgattt ctgcgactcc taaaccgagc gccgcaaaag gtcggaaccc
     3241 cagacgctcc aggggtgatt gaccgaacag ataggtgccg cgcagctccg cgtgcagagg
     3301 caaaaacttg tctccgttct tgggtgtggg ggcaccgccg aacgccacgc ccagccgggc
     3361 tccgagcagc cagcgtttcg cgaaaactcg atcgaaaccg atgagcaagc gagtgctcgc
     3421 cagcccgaat cccgcggcag tccggttgcc ttccccatcc caaatcgggt ccgtgtattc
     3481 tacacgaccg ctgccgaagc aggtgtagct cgcgccgtag caggccggat tggtcgacgg
     3541 atgccacagc atgtcttgct gcaagtgcaa cgacacccaa tagcggtggc cggtcacctg
     3601 ctcgggcgga gtggcctcgg gttcccctag ttccgccgcc ggggcctccg ccgtaggctc
     3661 ctcttcgagc actacttcct cggatttacc gccgccaacc tccttgaaca cgcgcgcgag
     3721 ttccggcgtg gtcaagtccg ggtcgagttg aatgaatcga tcgtaggaga cggcgcgtgc
     3781 cagggcctta cgccctttgg cttgtttgcc gagaccagcg acgtagacga cgcccaaatc
     3841 tcggtgcagt ttggccaaca cctgttgcga gcagccgtcc ttgccgcagc gctgaagcgc
     3901 tttgagcaat gtacgctccg cggcgcccgc ctttcccgcc aaatactcgg tgttcatggc
     3961 gcggtgctgc agctcggtgg cttctgcatc gcgggcgccg gcggcccttg acgaagaggc
     4021 gaacaagaag cccagcgaca agaagagcgt gcccgtgaag aagccagatg cgcgcatgag
     4081 atagaccaag ccggtcgacg tggattcaag gatcgccccg cgcgataccc ggctaaagga
     4141 agctgatttt ccggcccgaa ctaatcacga aggcgagcaa ctttcaatct cgactcgtcg
     4201 ctcgcctgac gcccgctctc ggtccgcgcg aaccaggaac ggactgagcc cgaagcgggg
     4261 gttcagcgag cgaacgagat gtcgtcgacc caaagctccg cgctgaacgt ggcgggcgcc
     4321 atgaactgca cctccaccag ttgactcgga tcgaacggct tcaccggcag gccccaatcg
     4381 ccctgctgaa aactactcca aagcagcgtg tactcttgcc aatccgtcga gagcgttact
     4441 tcccgcgaat agtggtcatt gcagagctta ccggcagtgt catcgcaggc accccgcgcg
     4501 gccagcagag tgtcacgcag cggaaggcgc accaacacgg tggcttcctc gccctgcgcg
     4561 cgcgcccaga aatggatgcc tttgtacttg gtggcatcgt acgggaattt tttctgcccg
     4621 gcatccgtgg actggatcat ttccacgccc atcaaggcac cctgagaacc ggcatctccc
     4681 ccgaatagcg tgaaatgcag cgcgtgctgg ctgccgggag cggttccctt ctctcccgtg
     4741 ggtagagatg ccatcacggg cgtcggagat ggcgtcaccg tggctccctt ctcggcgtcc
     4801 gcccacggga accactgccc gttgcgaccg tcgttcggga ggatgaagtg atcaaccttc
     4861 tccatgtcgt cgatcagctc ggggggccac gtggttgctc cgccggtgct cgcgccgccc
     4921 gttccgcccg tggcgactcc gccgctactc gtgccgccgc tcgacttgcc gccgctcgac
     4981 ggtgccccgc ccttcgcggc gccgcccgaa cccatgcccc ccgagcccgc agtgttcggc
     5041 gctccgcctt gagaacgacc gccagagctc actcctcccg aactcgggcg tgtgccggtt
     5101 ccggaagcgc ccgaggatgc gcctccggtg tcagaactgt cccctgccgc ggccatgccg
//
LOCUS       DASTKU010000996        11569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1536568, whole genome shotgun sequence.
ACCESSION   DASTKU010000996 DASTKU010000000
VERSION     DASTKU010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11569
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536568"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="VFQ61_35375"
     CDS             <1..1292
                     /locus_tag="VFQ61_35375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9959844.1"
                     /translation="LELDRRILAGLRDPFLHIIRNCIDHGIEPTAERTRAGKSVPALV
                     QVQIGYVRAGRVAIRISDDGRGIDLEQVKRAASKARVIGDEELAGLAPDAIRQLVFKS
                     DVSTSPWITDLSGRGLGLAIVRESVEKLGGSVNIESERGKGTRLTLELPASLASFRAV
                     VVGVAGHLFAIPSTGVRRVLRVQPSELTTIENRSVLRLDATAIGLVPLAKLFSLSGPA
                     REQSQALHAVLLAVAGEELAVLVDEIVEELDIPVKSLGEELEAVRIALGATLLGNGRV
                     APILDPAELLQSASKWLESGGAVARSQAETAERRASILVAEDSITSRTLLKGILESAG
                     FLVTTAVDGADAFAKLRSEKFDLVVSDVDMPRLNGFGLTERIRADRDLTDLPVVLVTS
                     LDSHEDRERGIEVGANAYIVKRSFEQSNLLAVIRRLI"
     gene            1552..2325
                     /locus_tag="VFQ61_35380"
     CDS             1552..2325
                     /locus_tag="VFQ61_35380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959845.1"
                     /translation="MLSSLRISILLGFVAAAVSACSSDSEDAPAQRTREAFCRDWAQA
                     ACSEQTVSACQAASPEACRASQVTFCESLVPSTFSDTQGDKCIQAVKDAYSDADLTAT
                     ELTTVLKLDGACAVIVRGNRGKGESCSSTRDCDASTGYECVIRGGQTSGTCQIPEEVG
                     AGQRCSALQQVCGAGFYCDGSNCIAAKEAGDSCVSDVECGEAGLCAGSECVDRAKVGS
                     ACTADEECISGICYTVNGAGSCVDRIRLSPAESACQDLR"
     gene            3241..4908
                     /locus_tag="VFQ61_35385"
     CDS             3241..4908
                     /locus_tag="VFQ61_35385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9959846.1"
                     /translation="MSPARLRSSKYRPLFELGRGGMSRVHLAEMRGAGGAAGDAPRLV
                     VLKELEQTLQGEPAMRAAMRLEAELSAGLNHPNVVRIFEVLECESSPVLVMEYLDGMP
                     LSQVLRQSRFALPLELHLHVLIQMLGGLHCFHELKSREGARLNPVHRDVSPQNTFILH
                     DGGVKLLDFGIAKVPSLHLPSTGTGLFKGKIQYMPPEQLFAERDIDRRADVFAAGIML
                     WEAVARRRMWSKASVSQIAKAIASGAIPSIREAAPDAPPFLIDIVERATQHERSERYA
                     SAQEMRAALQEALAEIGTASTRYRESEALAEFMRGYFGEKRAERRRSIEWYKSRNEAS
                     GVAVVPAAAADYGVAPPTPAPCSYELPSPKDGNPFAERRSVTEARSLWMALTVMGLGL
                     GVGLMLGLVRGPSRTPRLAVAAAQTSVSFSIAASPATASLRLDGQLLPSNPYTGRVPR
                     VTREATLEVSAPGHHTERRTLRFDHDTSISIALERDPARSGASRPTKSSDGISGRAPR
                     SRSGANVASERKPASSRSAASSCTPPFYFSEEGSKIYRPECFRRLPR"
     gene            4961..6415
                     /gene="gnd"
                     /locus_tag="VFQ61_35390"
     CDS             4961..6415
                     /gene="gnd"
                     /locus_tag="VFQ61_35390"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HET9959847.1"
                     /translation="MSEPRADIALIGLAVMGQNLVMNMSDHGFTVSVYNRTSAVTDEF
                     LSTTARDAKVIGHKSTRELIASLKRPRRIVILVKAGAAVDQVIEQLAPDLEPGDIVID
                     GGNSHFSDTERRFTALAQRGLRFVGSGVSGGEEGARLGPSIMPGGDAEAWPHLREIFQ
                     SIAAKVGDEACCNWVGKGGAGHYVKMVHNGIEYGDMQLIAEAYQLLKEGLGLEASALR
                     DVFDTWNRGVLDSYLIEVTRDVLGVKDEDGTPLVDKILDSAGQKGTGKWTSNSALDLG
                     VPVTLIGEAVFARALSFMKDERGRASQRLVGPSARYSGEAKEFIDHVEQALYAAKIVS
                     YAQGYMLLKEASRNYGFGLNFGDIALMWRGGCIIRSRFLGDIKQAFDKNPELENLLLD
                     DFFRAALERAQRGFRKTVAQAVELGIPVPAMSAALAFYDGYRRAVLPANLIQAQRDYF
                     GAHTYERTDKPRGEFFHTNWTGRGGHVSSRSYNA"
     gene            complement(6451..8211)
                     /locus_tag="VFQ61_35395"
     CDS             complement(6451..8211)
                     /locus_tag="VFQ61_35395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959848.1"
                     /translation="MLRRPPEPESQEISLYISTYYSLLRSSGEVRVRAFEEAHAFSGS
                     SLHVGALSDAADVSAFAYAAARLPAEMLQVDRIVLAQSHESFEAAGYDVRHFAPVKTR
                     GRRRPLRFDGAHTLCAFIASVSDIDDLIPIVTAYQIEWNKMHERLRRVATPELPELEN
                     LLDPLWLGQALGVSPADAQKLLAALAPNRPEAALQTLATRELDLRVRVLSPSHSQYQR
                     AAQRWWSGVEPVYLQKQAPRRPPVYFVSSNTHAIANLIGGYARKHESHLIDFARTANP
                     EGLGDEVEAALKDPDPTGLHSLLYYLLRAYIHSDPGHPKIEAVREFEQAQGLVHLSEP
                     GHIDVDAQLARLSELDPTLLDPRLEMPGLELLKQSDASILNIDYPLGMAAYHLLSRVG
                     QGAGDLRGIYVMGKAATLNGRVGDVMLSSNIYDEHSKNTFLIRNALTAQSVAPYLRYG
                     SVFDNQKAVTVRSAFLQNKQYMDAFYRSGYTVLEMEAGPFLGAVYELSNPQRMPSDEV
                     VHMSASTPFDLGILHYASDTPYSRRQSLLSKSLSYFGVDSTYACAISIVRRILELECA
                     RLASALPTNRDTTVSMTPSP"
     gene            9119..9532
                     /locus_tag="VFQ61_35400"
     CDS             9119..9532
                     /locus_tag="VFQ61_35400"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9959849.1"
                     /translation="MSRLVIQSESPPQAVFSTPFASGGAESPWAGGVDGSFAPAFEVW
                     ETERTLVIRADVPGVKPEDLSLFLEDSRVTICGRRKQDDLRLSLRCRAYERTYGAFWR
                     SFRMSPMHRATRARASLVLGVLTVVVHQSPALEPS"
     gene            complement(10344..>11569)
                     /locus_tag="VFQ61_35405"
     CDS             complement(10344..>11569)
                     /locus_tag="VFQ61_35405"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959850.1"
                     /translation="CGQNRHGPCQQQASAGGILIQSWLRHFSYDEAQLRDDTSMANVL
                     VLGAGMMGSAFCFPLCDRRHSVRLVGTHLDAEIVHSIRSSGVHPKLNVKLPGELRAFP
                     IEELETALHDVDAIVLGVSSAGVHWAAQQLSPHLCRTDTPVAMISKGLAFDGEQLITL
                     PDAFARALPPPAAARVQPVGVAGPCIAGELARRVPTCAVFVSRSADSRRTFADLCEAP
                     YYKVFTRPDVVGVEACAALKNAYAMGVALGTGIHLANGGEPGSVAFHNYESAVFAQAI
                     WEMQRVIRHMGGDPAQASWLPGVGDLDVTCNGGRTGRFGQLLGTGLGPREAQDRMSGA
                     TLECLEILAVLRIALAHFRSRSTTPGEFVGDAERVSGGAIEALPLLEHLAEIALDGKQ
                     VHMPFESFFGGSDRL"
BASE COUNT         2073 a         3305 c         3953 g         2238 t
ORIGIN      
        1 acttggagtt ggatcgccgc atcctcgcgg ggctgcgcga tccgttctta cacatcattc
       61 gaaactgcat cgatcacggc atcgagccga cggcagagcg cacccgggcc gggaagagcg
      121 tacccgcgct ggtgcaagtg cagattggct atgtgcgcgc ggggcgggtt gcgattcgaa
      181 tcagcgacga tgggcgcggt atcgacctcg agcaggtcaa gcgcgcggcc tcgaaagctc
      241 gagtgattgg cgacgaagag ctagccgggc tcgcgccgga cgcgatccgg cagctggtct
      301 tcaagtccga cgtcagcacc agcccatgga taaccgactt gtcaggccgc ggcttgggcc
      361 tcgcgatcgt ccgggagagt gtcgaaaaac tcgggggctc ggtcaatatc gagtctgagc
      421 gcggcaaggg gacgcgcttg acgctcgagc tgcccgcgtc gctcgcgagt tttcgggccg
      481 tggtcgtcgg cgttgcgggg cacctgttcg cgatcccgag cacgggtgta cgccgtgtgc
      541 ttcgcgtgca accgtccgag ctgacgacga tcgaaaaccg cagcgtgctc cggctggacg
      601 ccaccgcgat cgggctcgta ccgttggcga agctgttctc tttgagcggg cccgcgcgcg
      661 agcaaagcca ggcgctgcac gcggtgctcc tcgcggttgc aggcgaggaa ctcgcggttt
      721 tggtggacga gatcgtcgag gagctcgaca tcccagtcaa gtcgctgggg gaggagctgg
      781 aggcggtgcg gattgcgctc ggcgcgacgc tgctgggcaa cggcagagtc gcgccgattt
      841 tggatcctgc ggagctgctg cagagcgcga gcaagtggct cgaaagcggc ggagccgtgg
      901 ctcgaagtca ggcggaaact gccgagagac gcgcgtccat tctggttgcg gaggactcca
      961 tcacctcgcg cacgctcttg aaaggcattc tcgagagcgc gggctttctc gtgaccacgg
     1021 ccgtggatgg tgcggatgcg tttgccaaac tgcgttcgga gaagttcgac ttggtcgtct
     1081 ctgacgtaga catgccgcga ttgaatggct ttggtctgac ggagcgcatt cgcgccgatc
     1141 gggatttgac cgatctgccc gtcgtgctcg tcacgtcgct cgactctcat gaagatcggg
     1201 agcgcggcat cgaggtcggc gccaacgcct acatcgtaaa acgtagcttc gaacagagca
     1261 atttgctggc tgtgattcga cgcttgatct agggcgtcta ctccagtcgc tgcgagtgat
     1321 cgcgcagttc cgccccatgc ggcgcaggcc tgcgcgcgtg aaacgcccca atgtgccagt
     1381 ctcgcgcgcg agagctcgtg aaggcgcaga tcgcgagtcg agatgggctc attggtaaac
     1441 agctcaatca gcgatcatcc gcgtcgaccc gggttcatgt ctcgcttcag tggcacttag
     1501 cgctgcggag tccaaaaggc aggtaggtcc gccttttgca agcgcggtgg catgctttcg
     1561 agtttacgaa tttcgatcct tttggggttc gtggcagcgg cggtgtccgc gtgttcttcg
     1621 gattcggagg acgcccctgc ccaacgcacg cgtgaggctt tctgtcgaga ttgggcgcaa
     1681 gccgcgtgtt ccgagcaaac ggtgtccgcg tgccaagccg cgagcccgga ggcgtgtcgc
     1741 gcatcgcagg tcacgttctg tgagagcctg gtgccgtcga cgttctccga tacgcaggga
     1801 gacaagtgca ttcaggccgt gaaggatgcc tattccgacg cggatttgac ggcgaccgag
     1861 ctgacgacgg tgctcaagct ggacggcgcg tgcgcggtga tcgtgcgcgg aaatcgagga
     1921 aagggagagt cgtgctcgtc cacccgcgac tgcgacgcca gcactggcta cgagtgcgtc
     1981 attcgcggcg ggcagacgag tggaacctgt caaatccccg aagaggtagg tgctggacag
     2041 cggtgttccg cgcttcagca ggtctgtgga gcgggcttct actgcgatgg cagcaactgt
     2101 atcgccgcga aagaggcggg cgactcttgc gtcagcgacg tcgagtgcgg agaagctggg
     2161 ttgtgcgcgg gctccgagtg cgtggaccgc gcgaaggtcg gctccgcgtg tactgcggac
     2221 gaagagtgca tctccggcat ctgttacaca gtgaacggcg ctggatcttg cgtggatcgc
     2281 attcggctct ctcccgccga gtccgcctgt caggatctgc gctgagtagc agacccgcgt
     2341 tctctcgaag cgttggagtg ctgcgcgcag gcgttgcgct ggtaaacggc tgcttcgcgg
     2401 cggctcatga gcccgcgagg cagccgggtt tcgtcgacgg ggcgagctgc gttgagcggc
     2461 gcgggcgccc gacgcgtcga gtggcgaacc gcaaccgggt cgccccgtga gggcgagaga
     2521 aggggggcat gtcctgtcgc gctacaacga cgagtcgtcg agcggatgcc gatcggctcg
     2581 tgccggcgaa gaggggaggt gccagtgtcg tccgatttcg aaaagccctt cgtcgctcga
     2641 atcttctggg ggcctgcgcg gtgcacactc cggaatcacg agcgctccgc ctgcgctgcc
     2701 gggttgacta gtgagcgagc gcgctcgtcc cctcgtggcc aaagcagggg cacaatcgag
     2761 ggccaagctt ctaccgtaag cgcgacgaga gacagagcgc catcaaacag aagcgcggca
     2821 gttcacagag cgcgctggca aacagagcgc gtgcaaacgg agcgcgagca aacggagcgg
     2881 tagccatcaa aagagcgcag ccatcaaagg agcgcgatgg cagactgttg ggtgggagct
     2941 ctgcggagct gcgcttcgag cgcagctctg ggttcgacga gtgcgaacgt ggtgtgaaga
     3001 cccggtgagc gagacccaag agtgagtgtt acatgcggac tcgatgcatc gcgcggtgga
     3061 cgaggtcgtt acacggcggc gtcaatgaag tgggtgtttg gcgcgcaccg cgggtatttg
     3121 agggagaatt caggggtgcc gcgacaaaca gcgcgagcag tttggtctat cgatgccaga
     3181 ctgcggacta cgatgggtct gtcttcagat gaggctcgcg atcgttggcc gtcggaactg
     3241 gtgtcccctg cacgtttgcg atcgtccaag taccggcctt tgttcgaact cgggcgcggg
     3301 ggaatgtccc gcgttcattt ggctgaaatg cgcggcgctg gcggggcggc gggcgacgct
     3361 ccccgcctgg tcgtactgaa ggaactcgag caaaccctac agggtgagcc cgcgatgcgg
     3421 gcggcaatgc gcctagaagc ggagctgtcg gctggcctca atcatcccaa tgtggtccgg
     3481 atcttcgagg tcttggagtg cgagtcgagc ccagtgctgg tgatggaata tctcgacggg
     3541 atgccgctct ctcaggtctt gcggcagtcg cggttcgcct tgcccctcga gctgcacctt
     3601 cacgtcctga ttcagatgct gggcgggttg cactgctttc acgaactcaa gtcacgcgag
     3661 ggcgcacgtc tgaacccggt gcaccgcgac gtctcccccc agaacacatt cattctgcac
     3721 gatgggggcg tgaagttgct cgacttcggg atcgccaaag tgccgtcgct gcacctgccg
     3781 agcacgggaa cggggttgtt caaggggaag attcagtata tgccacccga gcaactgttt
     3841 gccgagcgcg acatcgatcg gcgggcggac gtctttgcgg ccggaatcat gttgtgggaa
     3901 gccgtcgcgc gtcggcgcat gtggtcgaag gcgagcgtct ctcagatcgc aaaagcgatc
     3961 gcgtccggcg ccattccgtc gattcgtgaa gcggctccag acgcgccgcc gttcctgatc
     4021 gacatcgtgg agcgagcgac gcaacacgag cgctccgagc gttacgcgag cgcccaggaa
     4081 atgcgagcgg ccctgcagga ggcgttggca gagatcggca ccgcctccac tcgatatcga
     4141 gagtcagagg cgctggcgga gttcatgcgc gggtacttcg gcgaaaagcg tgcggagcgg
     4201 cggcgcagta tcgaatggta caagtcccgc aacgaggcga gtggtgtggc ggtggtgccg
     4261 gcggccgcgg cggactatgg ggtggccccg cctactcccg cgccgtgttc atatgagctg
     4321 ccgagtccga aagatgggaa tccgtttgcg gaacggcgta gcgtaacgga ggcgcggagc
     4381 ctatggatgg cgctgacggt aatggggctc ggtctgggcg ttgggctcat gttggggctg
     4441 gtgcgggggc cgtcacggac tcctcgttta gcggtggccg ccgctcaaac cagcgtcagc
     4501 ttttcgatcg cggcctcgcc cgcgacggcg agcctgcgtt tggatggtca gctcttgcca
     4561 agtaatccgt acacgggtcg tgtgccccgc gtcacccgtg aagccacgct cgaggtcagt
     4621 gcacccggac accacacgga acgtcgcaca ctgcgcttcg accacgatac ctcgatcagc
     4681 attgcgctcg agcgggatcc ggcgcgatcc ggcgcctcgc gccccacgaa gtcttcggac
     4741 gggatttcgg gccgtgcgcc gcgcagccgc tcgggggcga acgtggcttc cgagcgtaaa
     4801 ccggcgtcga gtcgcagtgc ggcttcgagc tgcacgccgc cattctattt ctccgaagag
     4861 ggctcgaaaa tctaccggcc ggagtgtttc cgacgcctgc cgcggtgagt tgcgttggtt
     4921 tcttgtcgct atctcacatg cgctgcacta ggagcagagc atgtctgaac cgcgcgctga
     4981 cattgcactg attggcctgg ccgtgatggg tcagaacctc gtcatgaaca tgagtgacca
     5041 cggtttcacg gtctcggtct acaatcggac ctcggccgta acggacgagt tcctgtcgac
     5101 gaccgctcgt gacgccaagg tcatcgggca caagagcact cgtgagctga tcgcgtcact
     5161 gaagcggccg cgacgcatcg taatcctggt caaggccggt gccgcagtcg atcaggtcat
     5221 tgagcagctg gcgccggatc tggagcccgg cgatatcgtg attgatgggg gaaattcgca
     5281 cttctccgac accgagcgtc gcttcaccgc tctcgcccag cgcggcttgc gcttcgtagg
     5341 ttcgggcgtt tcgggcgggg aagagggggc tcggctcggg ccttcgatca tgccgggtgg
     5401 ggacgccgag gcgtggcccc acttgcgcga gatcttccag agcatcgcgg ccaaagtcgg
     5461 tgacgaggcg tgctgcaact gggtgggaaa gggcggggcc gggcactacg tgaagatggt
     5521 gcacaatggg atcgaatacg gcgacatgca gctgatcgcg gaggcgtacc agctgctcaa
     5581 agaggggctc ggtctcgagg cctccgcgtt acgcgatgtg ttcgatactt ggaaccgcgg
     5641 tgtgctggac agctacctga tcgaggtgac acgtgacgtg ctgggcgtca aagacgagga
     5701 cggtaccccg ctcgtcgaca agatcctgga cagcgcgggg cagaagggga caggcaagtg
     5761 gaccagcaac tcggcactcg atttgggcgt gccggtgacg ttgattgggg aagcggtctt
     5821 tgcgcgcgcg ttgtcgttca tgaaggatga gcgcgggcgc gcgagccaac gtttggtggg
     5881 cccgagcgca cgctactccg gcgaagcgaa ggagttcatc gaccatgtcg agcaggcgct
     5941 ttatgccgcc aagatcgtga gctacgccca aggctacatg ctgctgaagg aggccagtcg
     6001 gaactatgga ttcggcctga acttcgggga tattgcgctg atgtggcgcg gtggctgcat
     6061 cattcggagt cgattcctgg gggacatcaa gcaggctttc gacaagaatc ccgagctcga
     6121 aaatttgctg ttggacgact tcttccgcgc ggcgctggag cgcgcgcagc gcgggttccg
     6181 caagacggtg gcccaggcgg tagagcttgg gatcccggtg cccgcaatga gcgcggcctt
     6241 ggcgttttac gatggctatc gccgcgccgt gttgcctgcg aatctgatcc aggcgcagcg
     6301 tgactatttc ggcgcgcaca cgtacgagcg aacggacaag ccgcgcggtg agttctttca
     6361 cacgaactgg accgggcggg gtggccacgt cagctcgcga agttacaacg cttgaacggg
     6421 gtcggcgccg gatcgagcac ggagtatcga ttacggtgac ggggtcatag acacggtggt
     6481 gtcgcggttg gtcggtagag cgctggccag ccgcgcacac tccagctcga ggatgcgccg
     6541 cacgatagag atggcgcagg cataggtgct atctacgccg aagtagctca ggctcttcga
     6601 gagcagggat tggcgtcgag aatacggggt gtcggacgca tagtgcagaa tgccgaggtc
     6661 gaaaggcgtg ctggccgaca tgtggacgac ctcgtctgac ggcattcgtt ggggattact
     6721 cagctcgtac actgcaccca gaaacggccc cgcttccatc tcgagcacgg tgtagccgct
     6781 gcggtagaac gcatccatgt actgcttgtt ctgcagaaac gcgctgcgca ccgtgaccgc
     6841 cttctgattg tcgaacacgc tgccgtatct caagtaggga gccacgcttt gcgcggtcag
     6901 cgcgttgcga atcaggaacg tattcttcga gtgctcatcg taaatgttgc tgctgagcat
     6961 cacgtcaccc acgcgaccat tgagcgtggc ggccttgccc atgacgtaaa tcccacgcag
     7021 gtctccggcg ccttgtccca cacgcgaaag caaatggtac gcggccatgc cgagcgggta
     7081 gtcgatgttt aggatcgagg cgtcactttg cttcagcagc tcgagccctg gcatttccag
     7141 gcgtggatcg aggagagtgg ggtcgagctc ggagaggcgc gcgagctggg cgtctacgtc
     7201 gatgtgaccg ggctcggaca agtggacgag accttgcgcc tgctcgaact cgcgcacggc
     7261 ttcgatcttg gggtgaccgg gatccgagtg gatgtaggcg cggagtagat aataaaggag
     7321 cgagtgcaga ccggtgggat ccggatcttt caaggcggcc tcgacctcgt cgccgagccc
     7381 ttcgggattg gcggtgcgcg cgaagtcaat caggtgcgat tcgtgcttgc gtgcgtagcc
     7441 accaattagg ttggcgatgg cgtgagtgtt cgaagatacg aagtatacgg gtgggcgacg
     7501 gggcgcttgc ttttgcagat agacgggttc gacgccgctc caccagcgtt gtgcggctct
     7561 ctgatattgt gaatgggacg gtgagagtac acgaacgcgc agatcgagct cgcgggtcgc
     7621 gagggtttgt agggcggctt cggggcgatt gggagcgagc gcggcgagta acttctgggc
     7681 gtctgcgggg gaaacgccga gggcttgacc gagccacaag gggtcgagca agttctcgag
     7741 ttcgggcaat tcgggggtgg cgacgcgacg aaggcgctcg tgcatcttgt tccactcgat
     7801 ttgatacgcg gtcacgatcg ggatgagatc gtcgatgtcg ctgacgctgg cgatgaacgc
     7861 gcacagggtg tgggcgccgt cgaagcgtaa ggggcggcgg cggccgcggg tctttaccgg
     7921 ggcgaagtgc cgaacatcgt agccggcggc ctcgaacgac tcgtgcgact gtgcgaggac
     7981 gatccgatcg acttggagca tctctgcggg caacctggcg gccgcgtagg cgaaggctga
     8041 cacatcggcg gcgtcggaca gggcgccgac gtggaggctc gaaccgctaa aggcgtgcgc
     8101 ttcttcgaac gctcgcacgc gcacctcccc ggatgaccgg agcagcgagt agtaggtgga
     8161 gatgtagagg ctgatttcct gcgattcggg ttcgggtggc cggcgaagca tgggcgaggg
     8221 ccgatgatag tcacaagccg ctcgggttcg cgcgtcgttt cccacgatcg cccggtcgac
     8281 ccagccaagc tcagccggcc cctcacgcct ccaagaactc tcccgggata acctctcggc
     8341 gctttgattt cattgaaagc gcgcaacttt ctcaggaaga tctccgccgt tcagaactcc
     8401 cgctggtctt cccgggatgc ttggtgacgg gatgcttggt gacgcattgc ttgacggcag
     8461 gcgcggaagc acgagccacg atggtaggtg aagtgtggga gattgtgcgc gtacctcacg
     8521 cgcggatgcc ggagccgacc caaccctcgg tgccgatttc tcgcgacggt ccaggtcacc
     8581 cgcgtcccga gatgtctccc gggagagttt cggaatggcc cgctgccggc cattctagac
     8641 ctcgctctgc taagctggtc cccgcttagc ggaatccatt gacagagccg cttagcggaa
     8701 tgggatagac agcgcccttc tcagtcgctc ctactggcag gggctcttaa cctgagagcg
     8761 ggtcccgaat tgtctaaggc aagtcgaaga ctgaatatcc atgttttgga ttgtcgagtt
     8821 cctagggtta ccgtaaccaa acttgtttcg cataggtttc tgctgaattt tcgagggctt
     8881 gaaggatgcg gcgggcgcat gttttgctcg ttcttcgaga ggagcacgca tgcggtgcgc
     8941 cgtgctacct tgtgcgggag cgcgcgttcc aagtgccgcg gctccttggc ttcacggaat
     9001 acgaattgct tagcgggagt gattcgtagc agtgactcag ttcagtttct cggtggagta
     9061 gtcgacctga gtacagtcga cctgagtaac gaaacagtga gctcggtgag gagagcccat
     9121 gtctagattg gtaattcaaa gcgaaagccc gccgcaggcc gtcttcagca ctccgttcgc
     9181 atccggagga gcggagtctc cgtgggcagg gggggtggac ggaagctttg ctcctgcctt
     9241 cgaggtctgg gaaaccgagc gcacgctcgt aattcgggca gacgttccgg gcgtcaaacc
     9301 cgaggacctc tcgcttttcc tggaggattc ccgggtcacg atctgcgggc gccgcaaaca
     9361 ggacgatttg aggctttcgc ttcggtgtcg ggcttacgag cggacctatg gcgcgttttg
     9421 gcggagcttc agaatgtcgc ccatgcaccg ggctacgcgg gcgagagcct ctctggtgct
     9481 cggcgtcttg accgtggtcg tgcaccagag tcccgctttg gagccatctt gaggcctccc
     9541 acttagggga agccagggaa gattgggctt ccggccggtg ggttcgcctg ccgagccgcg
     9601 cctcatggta caaccctggt gatgccgccg ttcgcgaaag tttggctgcg ccgcgcgctc
     9661 ctgctgggcc acggcatagt gctgttgggt tcgtgcggcc gcgagacgtt cgacttattg
     9721 ccgcctgcgc ccatcagtag tggcggcgcc tccggggaga cacaaaactc cgggggtcag
     9781 agttccgggg gagtcgccga ggcgggacgc ggaggcatgt ccgcgccgag tggcggcgcc
     9841 gagtcgggag ggcgcagttc agttccctct ggagggcaag gagggcgcac tgacacctac
     9901 tgtaagcagg aattttgctc cacctgcccg ccgtgtatca agccggatga aacgtgcttt
     9961 ggcaatgata tttgcgagtg cggcgtctgt gtggagtgtt ccgagcgtta tccttgcccc
    10021 ggggatctcc gctgcgaccc agtcactcga cagtgcaagc ctccatgttc gtcctcggct
    10081 cagtgtacct ccgaccccta ttcgttctgt gacccgcagc gggggatctg cgtacaatgc
    10141 ttgatgagca gcgattgtgc gccgttggct gaaacatgca tgttcgggtt ttgcatccag
    10201 tgctccactg acgagttttg tcggaagaca cccgaaccct attgttacaa tggccgatgt
    10261 cgatcgtttc aatgcgtggg agacttcgat tgtgcggggc gtaggtgcat agacggtaag
    10321 tgtcgcgact agcaccctcg gcttcaaagg cggtcactac cgccgaagaa tgactcgaac
    10381 ggcatgtgaa cttgcttgcc gtccagcgca atttcggcca ggtgctcgag aagcggcaag
    10441 gcctctatgg ccccgccgct cacccgttct gcgtccccca cgaactctcc cggggttgtg
    10501 ctgcgcgagc ggaagtgggc gagggctatt cgtaaaactg caagtatttc caggcattca
    10561 agagtggcac cggacatgcg gtcttgcgcc tcccgcggac cgagtcccgt tccgagcagc
    10621 tggccaaagc gtccggtccg gccgccgtta caggttacat cgaggtctcc aacgccgggc
    10681 aaccaactcg cctgagcggg gtctcctccc atgtgcctta ttactctctg catctcccag
    10741 attgcctgtg cgaaaacagc agactcatag ttgtgaaaag cgacactccc gggttcacct
    10801 ccattcgcca agtggattcc ggtgcccagc gcaacgccca tggcgtaggc attcttcaag
    10861 gctgcgcagg cctcgacgcc gaccacgtcg gggcgggtaa acactttgta gtaaggcgct
    10921 tcacacaagt cggcgaacgt gcgccggcta tcggcactcc gagaaacgaa aactgcgcag
    10981 gtgggcactc tcctggcgag ttcgccagcg atgcaggggc ctgcaacgcc gacgggttgc
    11041 acacgcgctg cggccggcgg cggaagcgcg cgcgcgaagg cgtctggcaa ggtgatgagc
    11101 tgttcgccat cgaaggccag gcccttggag atcatggcta cgggcgtgtc cgttcggcat
    11161 aggtgcgggg acagctgctg ggccgcccaa tgcacgccgg cagagcttac gccgagcacg
    11221 atcgcgtcta cgtcgtgcag ggcggtctcc agctcttcga tggggaacgc ccgcaactct
    11281 cccgggagct tcacgttcag cttgggatga acgccagagc tgcgaatcga gtgcacaatt
    11341 tcagcgtcga ggtgagtgcc caccaggcga acgctgtgtc tcctgtcgca caacggaaag
    11401 caaaacgccg atcccatcat gccggcgcct aagaccaata cgtttgccat gctcgtgtcg
    11461 tcccttaatt gcgcttcgtc gtagctgaag tggcggagcc agctttggat gaggatgccg
    11521 ccggcggagg cttgttgttg gcaggggccg tggcggtttt gcccgcact
//
LOCUS       DASTKU010000997         6190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1759043, whole genome shotgun sequence.
ACCESSION   DASTKU010000997 DASTKU010000000
VERSION     DASTKU010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759043"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFQ61_35410"
     CDS             <1..189
                     /locus_tag="VFQ61_35410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959851.1"
                     /translation="DPSGNLYHCAQVNRGVRHNVVELGVEEAFRRLEREGCSQCWCAR
                     VVEENYAWGGRFDLSLPD"
     gene            complement(234..1385)
                     /locus_tag="VFQ61_35415"
     CDS             complement(234..1385)
                     /locus_tag="VFQ61_35415"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HET9959852.1"
                     /translation="MTLAQVPSEVTIRLDFATRDALTAFPRGAQSVLAAAHAGNHEGA
                     RAAGAPRSLGSTTWLEDGIQLTQSSAACTCESARWLPRTGRSVAVEGGAVGADATDGV
                     MIEARYRCPESSPIELTLPFIARLPPAHRHEARITRGAATQHELLFGASARLRLVPFE
                     SPDESVLRAQERSARTSMLQTWSLRRALGFMRLGFSHILSGADHIAFVLALVVGLRGR
                     RTLLAALTAFTIAHSLTLGLGVSGLLALPPSWVEPAIALSVVYTGFENLRGSGTTPRV
                     LTTFVFGLLHGSGFAGALTELALPSAELGAALLSFNVGVELGQLVVVALTLPVFWLAS
                     RRRSLRPPNGAKESSSEAMKPSGLESLAIRPVVSLMLMACGLFWFGTRL"
     gene            2427..4010
                     /locus_tag="VFQ61_35420"
     CDS             2427..4010
                     /locus_tag="VFQ61_35420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959853.1"
                     /translation="MSFRRTAVLIGIAALWNVTSANAQTPAAAPPAPAPAPPPGTTPV
                     PTPEPARTPGPGDTGPAPAPAREAGEPAPTPPATSAPAAAETPVAPPIGAAPPAPQGE
                     LPKPPSAPSTPTVSKWKVTIYGMVEFNAMHDSTQSLGESVGGTALRRKETFAGSHSRM
                     HFTSRNSRFGVKVNAPDFGDVKTSGVMEMDFFGYTAASEAAQVSSGTFRLRQAFVKMD
                     TPVVDLLVGQTYHVFGWQPFFFPATTSFFPIMNMVFGRMPQFRVSKTVKSDAVTFDIA
                     AAAVRPPQRDSGTPDGQAGLKLGINSWKGIHSLGAGTMLNDALSIGVSGLYRHFRLPE
                     NTAPQGAQRTTNGYGLSIDAFIPVIPAASVDDAGNALSLTGSFSISKGAADVLGVMQA
                     NNLPTTVAITNADGTPGTTPYVGAIDAGLVRFNTEGKLKPIGWQTFMVGAQYYLPPSG
                     SVFISANYTQSESKDITTANLFANTVYNKGRYIDGNILIDFTKAFRLGLSYQYSWQRF
                     TDGATAKNHRKEISAYMFF"
     gene            4225..5454
                     /locus_tag="VFQ61_35425"
     CDS             4225..5454
                     /locus_tag="VFQ61_35425"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET9959854.1"
                     /translation="MNTHRRKLVQSLGLVGCWSALVGLGLTGCKKKEEQPAAPAKAEV
                     SAARNEPVVINVVDVGGGLQLLQAAIESFQAKNPRSVEKFNFTKAPSPELPGKLKAMQ
                     SAGRMDLDLVLGGTDVLAAGVEQGLWTQLFPDHEAKFPGVLDNYLPAAREMQKLAKDQ
                     ALAVVFMPAGPLLEFNPDKVKTPPTSPAELLAWAKAHPKRFIYPRPASSGPGRTFLMG
                     LPYLLGDKDPKNPLTGWDNTWKYLKELAQYIEYYPASTGATMKELGEGSRDMTVTVTG
                     WDLNPRALGIVPKQFKVAAMKGMTWVNDTQYLMIPKGVAKEKLPIVLELVGYLLKPEQ
                     QALTYDKGYFYPGPAVKNVPLSMAPAESQNVIKEFGRPEYDEWIANFPHAQPLDAKML
                     VEAFKRWDEEIGVKTAK"
     gene            5650..>6190
                     /locus_tag="VFQ61_35430"
     CDS             5650..>6190
                     /locus_tag="VFQ61_35430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9959855.1"
                     /translation="MQQDFSQLRLHAVTRRFSAAPGAPAALEGLTLTVERGEFISLLG
                     PSGCGKSTALNCIAGLLSLSGGSIWLDERRIDVLSPEKRDFGMVFQNYALFPHMSVDR
                     NVGFGLKMRGVPVPELKTRVAKALSLVQLEGYEHKLPGQLSGGQQQRVAIARAIVVEP
                     ALVLMDEPLSNLDAKLRLEM"
BASE COUNT         1198 a         1883 c         1930 g         1179 t
ORIGIN      
        1 gaccccagcg gcaacttgta tcactgcgcg caagtgaacc ggggtgtgcg gcacaacgta
       61 gtagagctcg gggtcgagga ggcctttcgt cgcctcgagc gcgaggggtg ctctcagtgc
      121 tggtgcgcgc gcgtcgtgga ggagaactac gcgtggggcg ggcgcttcga cttgtctctg
      181 cccgattgag tgcctgattg gccacagacg tgccgggtca cgacgtcacg atcctacagc
      241 cgcgtgccga accagaataa tccgcaagcc atcagcatca gggagaccac cgggcgaatt
      301 gcgaggctct cgagtccact tggcttcatg gcttcggagc tcgattcttt ggccccgttc
      361 ggcggtcgca acgagcggcg cctcgacgcc agccagaaca ccgggagcgt cagcgcgacc
      421 acgaccagct gcccgagctc cactccgacg ttgaacgaga gcaaggctgc gccgagctcc
      481 gcggaaggga gcgcgagttc ggtgagagcc cccgcgaatc cggagccgtg caacaagccg
      541 aacacgaacg tagtcagtac tcgcggtgtg gtcccgctgc cgcgcaggtt ctcgaagccg
      601 gtatagacta cggacaacgc gatcgccggt tcgacccagc tcgggggcag cgcgagcaac
      661 ccgctgacac ccaagcccaa ggtcagagag tgggcgatgg tgaacgcggt cagcgccgcg
      721 agcagagtgc gacgaccgcg gaggccgacg accagcgcca gtacgaacgc gatgtgatcg
      781 gcgccgctca gaatatgtga gaagcccaaa cgcatgaacc cgagcgcgcg ccgcaagctc
      841 caggtctgca gcatcgaggt tcgcgcgctc cgttcttgcg ctcgcaaaac actctcgtcg
      901 gggctctcga atggcacgag gcgcagccgc gcgctggctc cgaataggag ctcgtgttgg
      961 gtcgcggccc ctcgtgtgat gcgggcttcg tggcgatggg cgggcgggag gcgagcgatg
     1021 aacggcagtg tgagttcgat tgggctcgac tccgggcagc gatatcgcgc ctcgatcatg
     1081 actccgtcgg tcgcgtctgc gccgacggct ccgccctcca ccgctacgga tcgaccggtg
     1141 cggggcagcc aacgcgccga ctcgcaggtg caagcagcgg agctttgagt cagttggata
     1201 ccgtcttcga gccacgtcgt tgagccgagc gaacgagggg ctcccgctgc acgcgcgccc
     1261 tcgtggttgc ccgcgtgagc ggccgcgagg acactctgtg ctcctcgtgg gaaggccgtg
     1321 agtgcgtctc gggtcgcgaa gtcgaggcgt atcgtcacct cacttgggac ttgggcgaga
     1381 gtcatagttt gggaacgtgg gtggcgatct cggctctgcc aatctcgcgc ggtctgcgcg
     1441 ccatactcgc cgcgagagag cccgagttcg tgggcatggg ctcgattcca ccataggagt
     1501 gatataacca agaccaccag acttggaccc agcgggatcg cgctacgaat cgaatcgaga
     1561 agaccgtgca cggaggcgcc tggcatgtgt gcactcgagt acgcgcgagc cgccattccg
     1621 gatcacacgt ggagcgtgcg gctcgattgc tagcccgcct aacccggcgt tttatttccg
     1681 aaagggcgtg gccggctagt ccaaacgttt cggattgtcc atcctaaaac acgagcgggg
     1741 ctggccgttg cggccgagag ccagaattgg ctcgcggccc ccaataccgt tgccagcgcg
     1801 gatctcctcg cgagttaccc gcggccggtg aatcgatgtc gatgggatgt cactcgctgt
     1861 ttcatgaagt gactcgcgtc agggtgacat agctcgaaat ctggagtgag cacgatcact
     1921 gtacccatcc gaacggatga tccgtgcagc tttttcgtgc tgcccgtgaa accgagcgcg
     1981 cgaatagaaa cagccgttga atcccgcgaa ctgcggtatt gcgcgctgca tcttcaagtc
     2041 ctttgtggtg ctagcgtcgc agttttgacg aattggcgtc ggggtccgga atcgcgtgcc
     2101 gctccccaaa gcggcattct tcgcaacacg cgcgcagatc caagaattgc tctggcattc
     2161 atcacgaaaa ttgacgagta tgcgcgcaat cgttcggggc atgtccgagc caagtctccg
     2221 aaaggttcac ctcaagctct cgaaatcagg gctcccacag gggcgcccac ctgagatgaa
     2281 gtggttccga ccggagatga ggtggttcta accggagata aggttcaggc atgacggtgc
     2341 tttcccgagc tcgtgcaagc ttgggggtac tggtggacga gcggttcctt ttttaggcac
     2401 gtatcgcaaa cttcgagggc ctccagatga gttttcgcag aactgcggtg ttgattggca
     2461 ttgctgcgct ttggaatgtg acgagcgcaa acgcgcagac accagccgcg gcgcctcctg
     2521 cgccagcgcc agctccacct cccggcacta cccccgtacc cacgccagaa ccggccagaa
     2581 ccccaggccc tggagatacc gggcctgcgc cggctcccgc gcgcgaagca ggagagcccg
     2641 cgcctacgcc gccggccacc tcggcacccg cggccgctga aactcccgtt gcaccgccga
     2701 ttggtgcggc cccgcccgcg ccccaggggg agttgccgaa gccgccctcg gctccgagca
     2761 cacccaccgt atcgaagtgg aaagtcacca tttacggcat ggtggaattc aatgccatgc
     2821 atgactccac gcagtccctg ggcgaatctg tgggtggtac tgcgctgcgc cgcaaagaga
     2881 ccttcgcagg ttcgcactcg cgcatgcact tcacgagccg caactcgcgt ttcggcgtga
     2941 aggtcaacgc tcccgatttc ggggacgtga agacgagcgg cgtcatggag atggatttct
     3001 tcggatacac cgccgcatcc gaagcagcgc aggtcagcag cggcaccttc cgtttgcggc
     3061 aagccttcgt gaagatggat acgccggtcg tggatctgct ggtcggccag acctaccatg
     3121 tgttcggctg gcagccgttc ttcttccccg ccaccacctc gttcttcccg atcatgaaca
     3181 tggtgttcgg ccgtatgccg cagttccgtg tcagcaagac ggtgaaatcc gacgccgtca
     3241 ctttcgatat cgcggcggcc gcggttcgcc cgccacaacg tgactccggc actccggacg
     3301 gtcaggccgg cctcaagctc ggaatcaaca gctggaaggg catccattcg ctcggtgccg
     3361 gcaccatgct gaatgacgcg ctgtcaattg gcgtttccgg tctttaccgg catttccggc
     3421 tgcccgagaa cacggcgccc caaggcgcgc agcgtacgac caacggttac ggcctctcca
     3481 tcgacgcctt catcccggtg attcctgccg cttcggtcga tgacgcgggt aacgcgcttt
     3541 ccttgaccgg ctcgttctcg attagcaagg gcgcggcaga cgtgctcggc gtcatgcagg
     3601 ccaacaactt gccgaccacg gtcgcgatca cgaacgcgga cggcaccccg ggcacgacgc
     3661 cttacgtcgg tgcgatcgat gccggcttgg tccgtttcaa caccgagggc aagctgaagc
     3721 cgattggatg gcaaacgttc atggtcggcg cgcagtacta cctgcctccc tctgggtcag
     3781 tgttcatttc ggctaactac acccagagcg agtcgaagga catcacgacg gccaacctgt
     3841 ttgccaacac cgtgtacaac aagggccgct acatcgacgg caacatcttg atcgacttca
     3901 ccaaggcatt ccgccttggg ctctcgtatc agtactcctg gcagcgcttc acggacggcg
     3961 cgacggccaa gaatcaccgc aaagaaatct ctgcgtatat gttcttctag actcgagacc
     4021 acagctttgg gcctggattt cgcgacgaat ccaggcctgg agggggcgct cgcaaagcct
     4081 ggttcagcct gggctttgcg agtgcgagta aaaacttggt caactcgaga tccgcgggcg
     4141 cgatctcagg catatctacc cagggcttgc cgagttctcc attcgctgcc gaggacgaaa
     4201 tggcgcccga ggctcggagc acatatgaat acgcatagac gtaagttagt tcagtcgctt
     4261 ggccttgtgg gttgttggtc ggcactcgtg ggtctcggcc tcaccgggtg caagaagaag
     4321 gaagaacagc ctgccgctcc ggccaaggcc gaggtttcgg cggctcgcaa tgagccggtc
     4381 gtcatcaatg tggtggacgt cggagggggc ttgcagctgc ttcaagctgc tatcgagtcc
     4441 tttcaggcca agaatccgag gtccgtagag aagttcaact tcacgaaggc tccttccccc
     4501 gagttgcccg gcaagctgaa ggccatgcag tcagcgggtc ggatggattt ggacctggtg
     4561 ctgggtggga cggacgtgct cgcagcgggc gtcgaacagg ggttgtggac gcagctcttt
     4621 cctgaccacg aggccaagtt cccgggtgtg ctggataact atttgccagc ggctcgcgag
     4681 atgcagaagc tcgccaaaga tcaggctttg gcagtggttt tcatgcctgc tggtccgcta
     4741 ctcgagttca acccggacaa ggtgaaaaca ccccccacct cacccgcgga actgttggct
     4801 tgggccaaag cgcaccccaa gcgcttcatc tatccgcggc ccgcaagttc tggccccgga
     4861 cggaccttct taatgggatt gccgtattta cttggcgaca aggatcccaa aaatccgctg
     4921 accggttggg ataacacttg gaagtatctg aaggagctcg cgcaatacat cgagtattac
     4981 ccggcctcta ccggtgccac gatgaaggag ctgggagagg gctcccgcga tatgacggtc
     5041 acggtcacgg gctgggatct caacccgcgc gcgctcggta tcgtgccgaa acaattcaaa
     5101 gtcgcggcca tgaagggcat gacctgggtg aatgacactc agtacctcat gattcccaag
     5161 ggcgtggcca aagagaagct gccgattgtc ctggaactcg tgggttactt gctcaagccc
     5221 gagcagcagg cattgactta cgataagggc tacttctacc caggtcctgc cgtgaagaat
     5281 gttccgctga gcatggcgcc cgcggagagt cagaacgtaa tcaaagagtt tgggcggccc
     5341 gagtacgacg agtggattgc caatttcccc cacgcccagc cgctggatgc caaaatgctg
     5401 gtcgaggcat tcaagcgctg ggacgaagaa attggtgtga agactgccaa gtgaagggct
     5461 gaatccgaag actgcctaat gcaagcgtga aaggcccatc ctgcgcaggt gggcttttcg
     5521 tttttgtcac tccgtgatct cgcgtgtccg ttgatcgaag tcggacatca gcggtgattc
     5581 ccaatgtcgg agatgaaatc gagttgacct tgtcggcatc tgcagtatgc cgcgtatctc
     5641 agatcggcca tgcaacaaga cttctcccaa ttgcggctcc acgccgtcac gcgccgcttc
     5701 agtgccgccc ctggggcgcc ggccgcgctc gaaggactca ctctgacggt cgaacgggga
     5761 gagttcattt ctctgttggg accctcgggc tgtggcaaat ccaccgcgct gaactgcatc
     5821 gcgggtctgc tctcgctctc gggcgggagc atttggctcg acgaacgtcg gatagatgtg
     5881 ctgtcaccgg agaagcgtga cttcgggatg gtgtttcaga actacgctct attcccgcat
     5941 atgtccgtag accgcaacgt ggggttcggg ctcaagatgc ggggcgtacc tgtgcccgag
     6001 ctcaagaccc gggtggccaa ggcgctctcg ctcgtgcagc tcgagggcta cgagcacaag
     6061 ttgcccggcc agctatctgg tggtcaacag cagcgcgtgg caatcgcgcg cgccatcgtc
     6121 gtggagccgg cgctggtgtt gatggacgag ccgctctcga acttggacgc caagctgcgc
     6181 ctcgaaatgc
//
LOCUS       DASTKU010000998         8139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_641374, whole genome shotgun sequence.
ACCESSION   DASTKU010000998 DASTKU010000000
VERSION     DASTKU010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641374"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFQ61_35435"
     CDS             <1..759
                     /locus_tag="VFQ61_35435"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET9959856.1"
                     /translation="PKGMSLTRDGLRLLAKAEYTLAAHHELMAEAARGKGELAGRLRL
                     GAGSNTNNEAIGRLLTTLAERWPSIEVVLKHRTSQEVLAGIRDGTLDAGFYNEPGEPD
                     PDLATLEVAQFSIHLAAAPGLVTPNPELDWKFLAELAWIYPTESACCGRTAEQLFGAR
                     RFRPRRVISADRQDVTITLIASGVGVGLLHADTAREAVDRGELELLFEAERRISVRFA
                     SLASRALEPMIAAASSILRGPALALDRKTSLVDR"
     gene            complement(742..1248)
                     /locus_tag="VFQ61_35440"
     CDS             complement(742..1248)
                     /locus_tag="VFQ61_35440"
                     /inference="COORDINATES: protein motif:HMM:NF022606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 family protein"
                     /protein_id="HET9959857.1"
                     /translation="MNNAVEFLIRGSLIGVGATLTMDLWAALLRRFGIRSLDFRLLGR
                     WLGHIPRGRWWHESIARAAPVSGELWIGSVAHYSIGVSFAFASLTLFGIEWSRSPTLL
                     PSLLLGIATVVAPLFVLQPAFGAGVASSKTSAPLFNAFKSLVTHIVFGVGLFVAAHAT
                     VILLPINE"
     gene            complement(1435..2100)
                     /locus_tag="VFQ61_35445"
     CDS             complement(1435..2100)
                     /locus_tag="VFQ61_35445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET9959858.1"
                     /translation="MSRLRVNAFGISIDGYGAGPDQSLENPLGVGGLGLHQWAFGTKT
                     FQRMHGDFAEPLIGDRAGQEGTDDHFVVRSFENVGAWIMGRNMFGPIRGPWINEDWKG
                     WWGKNPPYHVPVFVLTHHAREPITMEGGTTFHFVTNGIQEALTRAKEAAQGKDVRLGG
                     GVATVREYLTAGLIDELHLVISPVLLGRGEHLLTGMDLVRMGYKCTEHVSSAAATHIV
                     LSK"
     gene            complement(2285..2614)
                     /locus_tag="VFQ61_35450"
     CDS             complement(2285..2614)
                     /locus_tag="VFQ61_35450"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET9959859.1"
                     /translation="MAFTSVCPATAVIEGGMRIFQVGKKSVLLVWPAGGELKAYRARC
                     PHADLPLNDATFDGQKITCTQHNWGFDCGSGKCVTHYVPNSLHRYEVRVDGDDVLVDL
                     GPARPAR"
     gene            3035..4342
                     /locus_tag="VFQ61_35455"
     CDS             3035..4342
                     /locus_tag="VFQ61_35455"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET9959860.1"
                     /translation="MMPLLLVSACAEEQIQARFSDDYRCHEDQVEVEALGGQRYRATG
                     CGRSATYQCLRDGVRDNVCAKESEPEPEPDASASVATRKLEAPPARRQNSIISLDLKL
                     DQRTTLKLRAAPAKQGELLQLGLARTEPRDTLEDCDFAWLADGRVGVSPTPVFARTSN
                     MQSSLRLDLNGTAARQLRRARQFALKVCDYKWSLDEEQLSRVHEFVRLYDEELAWSGG
                     AKQANDALLPPQGGWPGWTASAPPPVAIKTAKPLEGQELYKKLAPSVYQLEVQGESGL
                     AQGSGVAVSTSELATNCHVLTGARRIIIRQHGQESIGRILRSQPAADRCVLGADQPFN
                     PVQGVRSYSDLAVGEATFTLGSPHGLELSLANGILSGLRQVHDTRYVQTTAPISPGSS
                     GGGLFDAFGNLIGITTGMLVGEEHLNQSLNFAIAADSFFKKEP"
     gene            4422..5396
                     /locus_tag="VFQ61_35460"
     CDS             4422..5396
                     /locus_tag="VFQ61_35460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959861.1"
                     /translation="MHRRMVLAALAQLPLLSITSGARAAEAPNQPRLKSIEQDQLGTT
                     FYLGLPQAPFATQNYADDTVIVFVPRTYRFHAAEGVPAVVHFHGHNSSAKRAMVAHSL
                     REQLVDSRQNAVLIVPQLAVFAADSSCGRLAQAGGLAKLLGSALTHAARFGRTALTES
                     RFPDQAKLGRVCISAHSGGYHAAACSLRSGGVDVSETYLFDALYAESQTFRDWVLERP
                     FEPESRHKLVSYFTAGAATEPLNHGLRADLERRGVWVAQEQREGELSRRALSHAGAVF
                     VRTSVGHSAVTWETNALRDVLHASMLPRHLNSKWFAQRDGARPLERRR"
     gene            5653..5814
                     /locus_tag="VFQ61_35465"
     CDS             5653..5814
                     /locus_tag="VFQ61_35465"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HET9959862.1"
                     /translation="MGEEVKQFNVYLPADLVREVKHHAVETEQSLSAIVAAALRAYLE
                     KQPQKRSKK"
     gene            5811..6194
                     /locus_tag="VFQ61_35470"
     CDS             5811..6194
                     /locus_tag="VFQ61_35470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959863.1"
                     /translation="MTTDGIKGILIETHNWGKTVAFWKALGYELEFETDHHSGQLRHP
                     SGGPYLFIAERSENAPIRVQPMLWVADATGFVPPRSGSCSRPFEPQHWGVLESLIADP
                     DGREFSVQAPIGPSDATPDREKHHG"
     gene            6187..7044
                     /locus_tag="VFQ61_35475"
     CDS             6187..7044
                     /locus_tag="VFQ61_35475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9959864.1"
                     /translation="MAESSKAAPENTAARVALWRALHVQADAPPHVFEDVVGLALVGP
                     DADWQKRPDMSPFTRPFRASIVARARFVEDLLERQVATGVAQYVILGAGLDTFAQRRP
                     ELASRLRVFEIDQPGPQEWKRKRLLELGLGIPSFLRLVPVDFERGEAWLEQLAAAGFD
                     PTVPTLVASTGVSMYLTKAAIATTLSQVAALAPGSTLVMSFMLPIELAEPELRPGIEA
                     AARGARANGTPFVSFFTPDEMLSLARDAGFKKVEHVSAEALAERHFAGRTDGLRPPKN
                     SEELLVACT"
     gene            7110..7565
                     /locus_tag="VFQ61_35480"
     CDS             7110..7565
                     /locus_tag="VFQ61_35480"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET9959865.1"
                     /translation="MDELRRLTQRFFRRFGALSADSTPCGKPLPIAHAHALMILLNRG
                     ELSQQALGAELCIDKSNVARLCAKMARSRHLQQRSSEHDGRSRLVSLTARGTRLAQEV
                     ESASRQRFATLLDALPRTRRTDIVLALQQLVEALEEVPNVTPAQTGSSA"
     gene            7562..8131
                     /locus_tag="VFQ61_35485"
     CDS             7562..8131
                     /locus_tag="VFQ61_35485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959866.1"
                     /translation="MNRTLILSSLLVSAACAREQTAPPAGTSVSPLSSAPAARDQENV
                     HGKLDRMDARRPVPLLPMMANHQKENMRDHLVAVQEIVSALTSDDFAAIEQAASRIGF
                     SEQMGRMCTHMGAGAQGFADQALNFHHTADRVAAAAREHNRGRVLSELGATLQTCTAC
                     HATWKQQVVDEATWQRVATSAPMSREVAR"
BASE COUNT         1527 a         2593 c         2542 g         1477 t
ORIGIN      
        1 ccgaaaggca tgagcctgac gcgcgacggg ctgcggctgc tcgcgaaggc ggaatatacg
       61 ctcgccgcac atcacgagtt gatggccgag gccgctcgcg gcaagggaga gctcgccggt
      121 cggctccgct tgggcgcggg cagcaacacc aacaacgagg cgatcggcag gttgctcacc
      181 accctggccg aacgttggcc gagcatcgaa gtggtcctca aacaccggac ttcccaggag
      241 gtactcgcgg ggataagaga cgggaccttg gatgccggtt tctacaatga gccgggcgag
      301 cccgacccgg acttggcaac cctcgaggtc gctcagttct cgattcatct cgccgctgct
      361 cccggcctgg tcacacccaa tcccgagctc gactggaagt tcctcgcaga actagcgtgg
      421 atctacccga ccgagagcgc ttgctgcggc cgaaccgcgg agcagctgtt cggtgctcgc
      481 cgatttcgac cgcgtcgggt gattagcgcg gatcgtcagg atgtcaccat aacgctgatc
      541 gcgagtgggg tcggcgtggg gcttcttcat gctgacaccg cgcgcgaagc cgtagaccga
      601 ggcgagctcg agctgctctt cgaagccgag cgacgcatca gcgtgcgctt cgcatccctc
      661 gcgagccgcg cgctcgagcc catgatcgcc gccgcatcgt ccatcttgcg aggaccagcc
      721 ctcgctctgg accgtaagac gtcactcgtt gataggtagc aaaatcaccg tcgcgtgggc
      781 ggcgacgaac aggcccacgc caaatacgat gtgcgtgacc aagctcttga aagcattgaa
      841 caggggcgcc gaggttttcg acgaggcgac gcccgccccg aaagcgggtt gcaagacgaa
      901 caggggagca acgacggtcg cgatcccgag cagcagcgac ggaagcagcg tcggcgagcg
      961 cgaccactcg atcccaaaca acgtcaggga cgcgaacgcg aagctgactc cgatcgaata
     1021 gtgtgcgacc gagccgatcc agagttcgcc cgaaacggga gccgctcgag cgatgctttc
     1081 atgccaccag cgcccgcgcg gaatgtgacc gagccagcgc ccgagaagcc tgaaatccag
     1141 tgagcggatc ccgaagcgtc gcagcagggc cgcccaaaga tccatcgtga gggtggcccc
     1201 aacgccgatg agtgaaccgc gaatcaagaa ttctactgca ttgttcatac tccacctctt
     1261 tcgatcgcgc cgcgcccagc agatcgcgcg agagctccgt gttcgatggg gctcggtggg
     1321 ttcaacatcg ctcggggcga gggaacaatc caatcgttgt ttcggaggtg ggacctcgga
     1381 gaacctgagg tccctgactg gagctatatc gcgttagttc gccgccaccc gagcctactt
     1441 cgacagcacg atgtgcgtgg ccgcggcgga gctcacgtgc tcggtgcact tgtagcccat
     1501 tcggaccagg tccatccccg tcagcaggtg ctctcctctt ccgagcagca ctggggagat
     1561 cacgaggtgg agttcgtcga tcaacccggc cgtgagatac tcgcgaacgg tcgcaactcc
     1621 cccgccgagg cgaacatctt ttccctgcgc ggcttccttc gcgcgcgtca gcgcttcctg
     1681 gatgccgttg gtgacgaaat ggaacgtcgt tccaccctcc atggtgatgg gctcgcgggc
     1741 atgatgcgtg agcacgaata cgggcacgtg atagggcgga ttcttccccc accagccctt
     1801 ccaatcctcg ttgatccaag gcccccggat cgggccgaac atgttgcggc ccataatcca
     1861 ggcgccgacg ttctcgaaac tccgcactac gaagtggtcg tcagtgcctt cctgacccgc
     1921 cctgtctccg atgagcggtt cggcaaagtc gccgtgcatt cgctggaagg tcttggttcc
     1981 gaatgcccac tggtgcagcc caaggcctcc gaccccgagc gggttctcca aagactggtc
     2041 cggacccgct ccataaccgt cgatagagat tccgaaagcg ttgactcgta gcctcgacat
     2101 gcgcgttctc cttcatgggg cgctggcact agccgcgccc ggctgtggga ccttcttgag
     2161 aagcgacgcg cgagccaagc cgaaatcgac gagccgtcta ttctttttta cgcgagacct
     2221 tcttcaccag gggttcggcg ttccaaaggc caccacttag cccgggaatc ggccgcccac
     2281 ccagctaacg tgccggccga gctggtccca agtcgactag gacgtcgtcg ccatcgacac
     2341 gcacttcgta gcggtggagc gagtttggaa cgtagtgggt cacgcacttg ccactgccgc
     2401 agtcgaaccc ccagttgtgt tgggtgcagg tgatcttctg gccatcgaac gtcgcatcgt
     2461 tgagcggaag atccgcgtgc gggcaacgcg cgcggtacgc cttgagctca ccacctgcgg
     2521 gccaaaccaa gagcacgctc tttttgccaa cttggaagat gcgcatcccg ccctcaatca
     2581 cggcagtagc gggacatacg gaggtgaatg ccatcgagtt cgcctttccg aaggggaaag
     2641 ccacacctct ggcaatttgt gcgccgtgtc gccgacggct agttttgccg gcagtttcgc
     2701 cgtttggagg gtttgcgctc cgtcaaactg aagtctatca atgtcgtgaa catgacaagc
     2761 gagggatcat ccgaaccgcg ggccaccctt ttgcgagacc tcaacgtttg atgaggctcc
     2821 cccgtcgtcg attcatcgct tctcgattgt ctctctacga cccgaatccg ccgctccact
     2881 cctctcgagc gacacctgtt aggtgacgcc cgcacccggg ttggcgccgg gcgccaagtt
     2941 gggtaccttc gggccgatca ccttgtccca agcttcgctg attggatcca tggcccgaac
     3001 agaagcggtt ccctcccgat ggcgcgcagc cttgatgatg cctttactgt tggtttcggc
     3061 ctgcgccgaa gaacaaattc aagcccgctt cagcgacgat taccgatgtc atgaagacca
     3121 ggtggaggtg gaggcactcg gtggtcaacg ctaccgagcc acgggttgtg gccgcagtgc
     3181 gacttatcaa tgtttgcgtg acggcgtccg agacaacgtc tgcgcgaaag agagtgagcc
     3241 cgagccagag ccggatgcgt cagcgtcagt cgctacccga aagctcgagg cacccccggc
     3301 gcgacgccag aattcgatca tctcgctcga tttgaaactg gaccaacgga caactttgaa
     3361 gcttcgggcc gcgcccgcca aacaaggaga actcctgcag ctcggcttgg cccgcacgga
     3421 acctagagac accttggaag actgcgactt cgcgtggttg gcggacggtc gggtcggcgt
     3481 cagccctacc ccggtgtttg cgcgcaccag caatatgcag tcttctctcc gactcgattt
     3541 gaatggaacg gccgcgcgtc agctgcgccg ggcccgtcaa tttgccttaa aggtctgcga
     3601 ttacaagtgg tccctcgacg aagaacagtt gtcccgagtg catgagttcg tgcggctcta
     3661 tgatgaagag ctcgcctggt cgggcggggc caagcaggca aacgatgccc tgttgcctcc
     3721 gcagggcggc tggcccgggt ggacggcttc tgcgccgccg ccggtggcca ttaaaacggc
     3781 aaagccgctc gaagggcagg agctctacaa gaagctggct ccgagcgttt accagctcga
     3841 ggtgcaaggt gagtcggggc tggcgcaagg ctccggcgtc gcggtcagca cgagcgagct
     3901 agccaccaac tgtcatgtac tgacgggcgc acgccgcatc atcatccggc aacacggcca
     3961 ggaatcgatt ggacgcatcc tgcgcagcca acccgccgcc gatcgctgtg tgctcggcgc
     4021 ggaccaaccc ttcaaccccg tccaaggcgt gcgttcgtac tcagacttgg ccgtcggcga
     4081 ggctacgttc actctgggct cgccccacgg tctcgagctg agcttggcga acggaattct
     4141 ttctggtctt cggcaggtgc atgacacgag gtacgtgcaa acgacggcgc ccatttcgcc
     4201 cggctcttcc ggtggcggcc tgttcgacgc cttcggcaat ttgattggga tcacgaccgg
     4261 catgctggtc ggagaggagc atctcaacca atcgctgaat ttcgcgatcg cagcggattc
     4321 attcttcaag aaagaaccgt aaagccccgt cccggcccag tccgtcgccc gctgccgaaa
     4381 aacttggcca cattcaccca catgaggcaa gcgaggtcac catgcaccgc cggatggtct
     4441 tggccgcgct cgcgcaactg ccactgctct caatcacttc gggcgctcgc gcggcggaag
     4501 cgccgaatca gcctcggctg aagtcgatcg aacaagacca actcggcacg acgttttacc
     4561 tcgggctccc gcaagcgccg ttcgccacgc agaattacgc ggacgatacg gtgatcgtgt
     4621 tcgttccgag aacgtaccgt tttcacgcgg cggagggggt gcccgcggtc gtccattttc
     4681 acggccacaa cagcagcgcc aagcgcgcca tggtggctca ctcactgcgc gagcagctcg
     4741 tcgacagtcg tcaaaacgcg gtgctgatcg tgccgcagct cgcggtgttc gcggcggatt
     4801 cctcctgcgg caggctggct caggctggag gtttggcgaa acttctgggg agcgccctca
     4861 cccatgcggc ccgttttgga cggaccgccc tcactgagtc gcgtttcccc gatcaggcta
     4921 agctcggtcg agtctgcatt tcggcgcatt cgggtggtta tcacgctgcg gcctgcagcc
     4981 tgcgcagcgg cggcgtcgac gtgtcggaga cttacctgtt cgacgcgctg tacgcggaga
     5041 gccagacctt tcgcgactgg gtcctggagc ggcctttcga gccggagagc cgccataagc
     5101 tcgtgagcta cttcacggca ggcgctgcga ccgagccgct caaccacggt cttcgcgccg
     5161 atttggagcg acgcggagtg tgggtggcgc aggagcaacg tgagggcgag ctgtcacgcc
     5221 gggcgctctc ccacgctggc gccgtgttcg tgcgcacctc cgttgggcac tctgccgtaa
     5281 cttgggagac gaacgccctc cgcgacgtgc tgcatgcttc gatgcttcct cggcacctga
     5341 attcgaagtg gtttgcccag cgtgacggcg cgcgcccgct cgagcggcgt cgctgaataa
     5401 tcaccaacaa ggacccagcg gctcgagcca atcggctctc cgctcgacgg ctcgtcctat
     5461 ttgccacagg caaataaagt aagggcgcga caaggatggg gctggcctcg tgtgcatagc
     5521 ttgctagcat cctagcaagc tatgcggtca gatagacgag agttacgtgc cgtgccgacg
     5581 aattgcgccg cgccaaagac tcggctgcgt ggcagccgaa acgctgacag atcccgatgg
     5641 tgataggtcc ccatgggtga agaggtaaag cagttcaatg tctatttgcc ggccgatctc
     5701 gtccgcgagg tgaagcacca cgccgtcgag acggagcagt cgctgtcagc aatcgtggcc
     5761 gccgccttgc gagcatattt agagaaacaa cctcaaaagc ggagcaagaa atgaccaccg
     5821 atggcatcaa gggaatcctg atcgagacac ataactgggg taagacggtc gcgttctgga
     5881 aagcgctcgg gtacgagctg gagttcgaga cggaccatca ctctggccag cttcgacacc
     5941 cgagtggcgg gccatacctc ttcattgccg agaggtccga gaacgcaccc attcgagtgc
     6001 agcccatgct gtgggtcgcg gatgctacgg gtttcgtacc gccgaggagc ggctcctgtt
     6061 cacgcccatt cgaaccgcag cactgggggg tgctggagtc gttgattgcc gatcctgatg
     6121 gtcgtgagtt cagcgtgcag gcgcctatag ggccgagcga tgccactccg gaccgggaga
     6181 aacaccatgg ctgagagttc caaggccgcg cccgagaaca ccgcggcgcg ggtcgcgctg
     6241 tggcgggcgc tacatgttca ggccgacgca cctccacatg tcttcgaaga cgtggttggg
     6301 ctcgcgttgg tgggaccgga cgcggattgg cagaaacgac cggatatgag ccccttcacg
     6361 cggccctttc gcgcctccat cgttgcgcgc gcgcgtttcg tcgaggacct gctggagcgg
     6421 caagtcgcaa ctggcgtcgc gcagtacgtg atcctcggag cgggtctgga cacgtttgct
     6481 cagcgccgac ccgagctcgc ctctcgcctg cgcgtgttcg agatcgatca acccggtcct
     6541 caggagtgga agcgaaagcg acttctcgag ctggggctcg gaatcccgtc gttcctccga
     6601 ctggtgcccg tagatttcga gcgcggcgag gcatggttgg agcagctggc cgcggccggc
     6661 ttcgatccga cggtgccgac gctcgtcgct tcgaccgggg tgagcatgta cctgaccaag
     6721 gccgccatcg ccacgactct gagccaagtc gcggcacttg ctcccggctc caccctcgtc
     6781 atgtctttca tgctccccat cgagttggcg gagcccgagc tgcggcctgg gatcgaagcg
     6841 gcggcccgag gagcaagagc caacgggaca cccttcgtca gcttcttcac gccggacgag
     6901 atgctctcgc tcgcccgcga cgccggtttc aagaaggtgg agcacgtctc tgcggaagcc
     6961 ctcgctgaac gccacttcgc cgggcggact gacggtcttc gcccacccaa gaactcagag
     7021 gagctgctcg tggcctgcac atagagctgg gaacgagatg gttgcggtcg caactacttt
     7081 ctggtagcgg tacttgggtg caggatcccg tggatgagct tcgccgtctc acgcagcgct
     7141 tcttccggcg atttggtgcg ctctctgccg acagtacgcc gtgcggaaag ccgctcccta
     7201 tcgcgcacgc tcacgcgctg atgatcctgc tgaatcgggg ggagctatcc cagcaggcac
     7261 ttggggcaga gctgtgcatc gacaagagca acgtcgcgcg gctctgcgcg aagatggcgc
     7321 gctcgcgcca cctgcagcaa cgttcgagcg agcacgacgg acgcagccgg ctcgtctcgc
     7381 tcaccgctcg aggcacccga ctcgcgcagg aggtggagtc cgcgagcagg cagcgcttcg
     7441 cgacactgct ggacgccctc ccgaggaccc gacggaccga tatcgtgtta gcgctccagc
     7501 agctggtcga agcgctggaa gaggtcccta acgtcacgcc tgctcaaaca gggagttccg
     7561 catgaaccgc actctgatcc tgtcatcgct gctcgtgtca gcggcctgtg cgcgcgagca
     7621 aacggcacca cccgcgggca ccagcgtgag cccgctgagt tccgcgccgg ccgcgcgcga
     7681 ccaagagaac gtccacggta agctagacag gatggatgcg cgccggccgg tgccgctcct
     7741 accgatgatg gccaatcatc agaaggagaa catgcgtgat catttggtgg ccgtgcagga
     7801 gatcgtatcc gctcttacgt cggatgattt tgccgcgatc gagcaggccg cctcacgcat
     7861 cggtttctcg gagcagatgg gccgcatgtg cacgcatatg ggagcgggcg cacaaggctt
     7921 cgccgatcag gcgctcaact tccaccacac cgccgacagg gttgcggccg ccgcgcgcga
     7981 acacaaccgg ggacgcgtcc tgagtgagct cggggccacc ttacaaacct gcacggcgtg
     8041 ccacgccacc tggaagcagc aggtggtcga tgaagcgacc tggcagcgcg tggcgacgtc
     8101 ggcccccatg agtcgcgaag tggcgcgctg agctctggc
//
LOCUS       DASTKU010000999         5428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1984093, whole genome shotgun sequence.
ACCESSION   DASTKU010000999 DASTKU010000000
VERSION     DASTKU010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984093"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFQ61_35490"
     CDS             complement(<1..647)
                     /locus_tag="VFQ61_35490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959867.1"
                     /translation="MPLSGGESRSQSWAVSEPFQGTFRDLALARLNTLGQALPSRRVL
                     PLAGALLNLLDRWAELPTTALGRANLQADVEALGSVFEVGVCVGNRGLEGLRLMIQAE
                     GHPQLRGSNWAESLRFSLALRETFGANLELFDALAPVFTPSGLRPAFTLRHELLLLGP
                     TPSFRLHVVPREPGTRSTLVKLREALQRVGLSEAWEFVHPRMSHEAELLRLCVDLA"
     gene            831..1562
                     /locus_tag="VFQ61_35495"
     CDS             831..1562
                     /locus_tag="VFQ61_35495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9959868.1"
                     /translation="MSEALVLIEDLRKSFQHEGRTLDVLRGIDLNIYAGQILAIVGPS
                     GAGKSTLLHCMGTLDLPTAGRIRLAGEELTTMTGSRLAAVRNRTIGFVFQFHHLLPEF
                     SALENVMLPGLIQGRPRKEMERRAGALLEEVGLSHRITHRPGELSGGEQQRVAVARAL
                     ALDPKLLLADEPTGNLDTATSDAIHDLFFQINRDHNTTIVVVTHNPAFAERMPRVVRL
                     RDGRVEKDDVRSVGGLVDRGVVLPS"
     gene            complement(1572..2621)
                     /locus_tag="VFQ61_35500"
     CDS             complement(1572..2621)
                     /locus_tag="VFQ61_35500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HET9959869.1"
                     /translation="MLEMVSMLRHEVRGAMQRDEDELLALASFLNSVNLPHDRAHVRA
                     LLAHSEKSFRGELPTAHRKFVFLLRDLETQQAVGTSMIVAQLGRRDAPYIYLDVISEE
                     KYSHAVDKHFHHTVLKIGFSYDGPTEIGGLVVAPEHRRANERLGLLISYVRFLYIAAH
                     RELFQNEILAELLPPLEPDGTSHLWEALGRHFTDMSYAEADRLSSTDKTFIRDLFPSG
                     DIYASLLSKDAQSVIGKVGAQTRGVEKMLRRVGFRYAERIDPFDGGPHFVAPVEEISL
                     VQETRERRLHAVRAPSSASPRALVARDLEEAPFFRAVACPVELGADDAVIVEESVVNH
                     LALKPGERVYVLPLR"
     gene            2785..4056
                     /locus_tag="VFQ61_35505"
     CDS             2785..4056
                     /locus_tag="VFQ61_35505"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9959870.1"
                     /translation="MISQRVIVPRSSLSQPSMDTPTAWPFPRTDTLPPYGFGVTDAVK
                     ARVIAQGADLIDLGLGNPDRPIPPEIVERLQEAIGIAGNHRYFPGRGLEVLRRALAAW
                     YARRYGVGIDPEHEIVVTLGAKEGMSHLCLALLQAGDVAIVPTPCFPIHAGAPRIAGA
                     EVELYEVTSGLEPAAAIRRAIERVRARGKRPKLVIANYPHNPTGLSVTPEQMRELLEV
                     VERSGALLMHDLAYADCDFGARYAPSVFSSGLEPERVKRFAVEVFSMSKSYNMPGWRI
                     AFMAGNQRMIDALAHLKTYLDYGAFAPLQQAAAWALDHGDELASRFRHLYHTRAKALV
                     SGLREAGFSDAAEPSGTMFVWCRLPVPLAHQSSAQAACELIEHAHVATSAGSGFGPGG
                     EGFLRFALIQDPPRIREACRRLGLYLQPVGA"
     gene            complement(4060..5289)
                     /locus_tag="VFQ61_35510"
     CDS             complement(4060..5289)
                     /locus_tag="VFQ61_35510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959871.1"
                     /translation="MRKRGQRANGLHRNGSNGPWLVTMALLASCGSRSAAELSSLPEG
                     NSAGANATGGIGWVGGTMGRAGSSTRYPEGLGGIPSVAGGRNSGGNASGGNASGGKAS
                     GGTRTTGAPSAGAGGSAIGGASHGGDGGAGGEPIYFPPDCRGGPVLVDLRIEDDEQLE
                     RELSGRTRVVGTLELTGSVRDLEPLHCLERVEDLVIHDAPQLLSLRGLEQLTEIDGGL
                     ELLNLPVLENLIGLPVLQQVGSIELQHNPRLVDLTGLLALNEYSEMNVLIRDNAGLTS
                     LVGLHGVGSLNDLHLESNPLLMDLRGLDQLVAVHNLTLADLQGLRTVAGLDRLHFVKG
                     NLTISGNENLTDLRSLGAVDSVYSLLSITDNARLPTCRAEWLRDQIGITHIGTDSTSP
                     PPHYEISGNDDAATCTP"
BASE COUNT          993 a         1781 c         1668 g          986 t
ORIGIN      
        1 gccagatcga cgcacaagcg caagagctct gcctcgtgac tcatgcgcgg atgcacgaac
       61 tcccaagcct cgctcagacc aacccgctgc agcgcttcgc gcagcttgac cagcgtcgag
      121 cgcgtcccag gttcacgcgg caccacatgc aatcgaaagc tcggcgtagg acccagcagt
      181 agtagctcat gacgcagcgt gaacgccggt ctgagcccgc tcggagtgaa caccggtgcc
      241 agcgcgtcga acagttccag attcgcaccg aacgtctctc gaagcgcgag actgaagcgc
      301 aagctttctg cccaattgga gcctctcagc tggggatgcc cttcggcctg aatcatcagc
      361 cgtaggcctt cgagacctcg attgccgacg cagactccga cctcgaacac gctgccgagc
      421 gcctcgacgt ccgcctgcaa gttcgcgcgc cccagagccg tagtaggcaa ctcggcccaa
      481 cggtccaaca ggttcagaag agcaccggcc agaggcagca ctcgccggct cggcaacgct
      541 tggcccagcg tgttcaaacg cgccaatgcc aagtctcgaa acgtgccctg aaagggttca
      601 gaaaccgccc aactttgcga tctagattct ccgcccgaca ggggcatggc ccggactccc
      661 tcttatccca gacgcgctgc tcgcggaccg gggttctggc cgatgcgtac gaccgtggca
      721 acgcgcatca gtcactcgac ttaaggcccg gaccggtttt ttctgccaca gcctgacggc
      781 cgctgtgctc cccgggcggt cggtgatata ggcgcccgcg aggcatcccg ttgtccgaag
      841 ctttggttct gattgaagat ctgcgaaaga gctttcaaca cgaaggccgc actctcgacg
      901 tgttgcgagg catcgacctg aacatctacg ccgggcagat cttggcgatc gttgggccgt
      961 cgggcgcggg taagagcacc ctgcttcatt gcatgggcac gctggatttg cccaccgccg
     1021 ggcgcattcg cttggccggt gaagagctca ccaccatgac gggctcgcgc ttagccgcgg
     1081 ttcgaaaccg aaccatcggg ttcgtgttcc agttccatca tttgctgcct gaattctcag
     1141 ccctggagaa tgtcatgttg ccgggtctga ttcaggggcg accgcgaaaa gagatggaac
     1201 gccgtgcggg ggccttgctg gaggaggtgg gcttgtctca ccgaatcacg caccgccccg
     1261 gtgagctctc cggcggtgag caacaacgag tcgcggtcgc tcgcgcattg gctctggatc
     1321 cgaagctgct cttggcggac gagcctacgg gtaatctcga tacggccacc agcgacgcca
     1381 tccacgactt gttttttcaa atcaaccggg accacaacac cacgatcgtg gtcgtgacgc
     1441 acaatccggc tttcgcggag cggatgccgc gcgtggtgcg cctgcgcgat ggccgcgtcg
     1501 agaaggacga cgtccgcagc gtcggaggcc tggtcgatcg gggcgtggtt cttccgagct
     1561 agcggcgagc gtcaacgcaa cggcagtaca tacacgcgct caccgggctt gagcgccaaa
     1621 tgattgacga cgctctcctc cacaatcacc gcgtcgtctg cgcccagctc cacggggcaa
     1681 gcgactgcac ggaagaaggg agcttcctcg agatcgcgag ccaccagggc tcgcggggac
     1741 gcagagctcg gtgcgcgtac ggcgtggagg cgacgctcgc gcgtctcttg caccaagctg
     1801 atttcctcga ccggcgccac gaaatgcggg ccgccatcga acgggtcgat gcgctcggcg
     1861 taacgaaagc ccacccggcg tagcatcttc tccaccccgc gcgtctgagc tcccactttt
     1921 ccgatcacac tctgcgcatc cttactcaac aggctggcgt agatatctcc gctcggaaac
     1981 aggtcgcgga tgaaggtctt gtccgtgctc gagaggcggt cggcttcggc gtagctcatg
     2041 tccgtgaagt ggcgcccgag cgcctcccac aaatggctcg tgccatccgg ctccagcggg
     2101 ggcagcagct ccgccaagat ctcgttctgg aagagctcac ggtgagccgc gatgtacaag
     2161 aaccgcacgt aggaaatcag cagcccgagg cgctcgttcg cccggcgatg ctcgggcgcc
     2221 accacgagac cgccgatttc ggtgggcccg tcgtaagaga aaccaatctt caacaccgtg
     2281 tgatggaagt gcttatccac ggcgtgcgag tatttctcct cgctgatcac gtccagatag
     2341 atgtacggcg cgtctctccg tccgagctgc gccacgatca tggacgtgcc gaccgcttgc
     2401 tgggtctcca agtcgcgcag caagaacacg aacttgcggt gcgcagtcgg taactccccg
     2461 cgaaagctct tttcggagtg ggccagcagg gcgcgcacgt gcgcgcggtc gtgaggaaga
     2521 ttcacactgt tcaggaacga cgcgagcgcg aggagctcat cctcgtcgcg ctgcatggct
     2581 ccgcgcacct cgtgccgcaa catgctgacc atttctaaca ttttgcggtt cgtttccggc
     2641 gttttggcga acgggaactg tcattcgcct cggccgagag gcccggttgc ttggctagat
     2701 cgcgaatcca ccataaaacc cgaatggagc gcggtgcgcg agccccgtca cccacagcgc
     2761 cccgcattgt ttcccggggc ccccgtgata tcacagcgcg tgatcgtgcc gcgctcctcg
     2821 ctctcacaac cctcgatgga cacgccgacg gcttggccgt tcccacgcac cgatacgctg
     2881 ccgccctatg gattcggggt gaccgacgcg gtcaaggcgc gagtgatcgc gcagggtgcg
     2941 gacctgatcg atctggggct agggaaccca gatcggccaa tcccgcccga gatcgtcgag
     3001 cggttgcagg aggcgatcgg gatcgctggc aatcaccgct atttcccggg gcgtggactc
     3061 gaggtgctgc gccgcgcgct ggcggcttgg tatgcacgtc gctacggcgt cggaatcgat
     3121 cccgaacacg agattgtcgt gaccttgggc gcaaaagaag gcatgagcca tctgtgcctc
     3181 gcgctgttgc aggcggggga cgtggcaatc gtaccgacgc cttgtttccc aatccacgcg
     3241 ggtgcaccgc gcatcgcggg cgccgaagtg gagctatacg aggtcacgag cgggctcgag
     3301 ccggctgcgg ccatccgacg tgcgatcgaa cgcgtccggg cgcgtggcaa gcgccccaaa
     3361 ctggtgatcg ccaactatcc ccacaatccg actgggctca gcgttacgcc cgagcagatg
     3421 cgcgagctgc tcgaggtcgt cgagcgctcg ggtgccctgc tcatgcatga tttggcctat
     3481 gcggactgcg acttcggggc tcgctacgcg ccgagcgtgt tcagctccgg gctcgagccg
     3541 gagcgggtga agcgcttcgc cgtcgaagtg ttctcgatgt ccaagagcta caacatgccg
     3601 ggctggcgca tcgcattcat ggccggcaat cagcgcatga ttgacgcgct cgcgcatctg
     3661 aagacttact tggactacgg cgcgttcgcg ccgctgcaac aagccgcagc ctgggcgctc
     3721 gatcacggcg acgagctagc gtcccgattc cgacacctgt atcacacacg cgccaaggcc
     3781 ttggtttcgg gtctgcgcga ggctggcttc agtgacgccg ccgaaccgag cggcaccatg
     3841 ttcgtctggt gtcgacttcc agtgccgttg gctcaccaga gctccgcgca ggcagcttgc
     3901 gagctgatcg aacacgctca cgttgccacc tccgcgggat ccgggttcgg ccccggcgga
     3961 gaaggatttt tgcgcttcgc tctgatccaa gacccgccgc ggatccgaga ggcgtgtcga
     4021 aggctcggtc tctacttgca accggtcggc gcttaggcgc tacggggtgc acgtggccgc
     4081 gtcgtcgttg cccgaaatct cgtagtgggg tggtggcgac gtggagtccg ttccgatatg
     4141 agtgatgccg atctggtcac gcagccactc cgcgcggcag gttgggagcc gcgcattgtc
     4201 agtaatactc aggagtgaat agaccgaatc gactgcgccg agggaacgca agtctgtgag
     4261 gttctcgtta ccggaaatcg tgaggttccc cttcacgaag tggagccgat ccaaacccgc
     4321 gaccgtgcgc agcccctgca ggtcggccaa ggtcaaattg tgcaccgcca cgagctggtc
     4381 gaggccgcgc aaatccatca acaatgggtt cgattcaagg tgcaggtcat tcagactccc
     4441 gacaccatgc aagcccacca agctcgttag gcccgcgttg tcacggatca gcacgttcat
     4501 ttccgagtat tcgttcaacg ccaacagccc ggtaaggtcg acgaggcgcg ggttgtgctg
     4561 gagctcgatg gagccgacct gctggagcac cggtaagcca atcagattct cgagcaccgg
     4621 gaggttgagc agttccaggc caccatcgat ctcggtgagc tgctcgagcc cccgtaagct
     4681 gaggagctgc ggcgcgtcgt ggatcaccag atcctctact cgttctaaac agtgcagcgg
     4741 ctccagatcg cgaacgctac ccgtcaactc cagggtgccc acgactcgag ttcgaccact
     4801 cagctctcgc tcgagctgct cgtcatcttc aatccgcaag tccaccagca ccgggccccc
     4861 gcgacaatcc ggaggaaagt agattggctc gccacccgca ccaccatccc cgccgtggct
     4921 ggcgcctcca atcgcggagc cgccggcgcc ggcgcttggc gcccccgtcg tgcgcgtacc
     4981 gccggaggct ttgccgccgg aagcattgcc gccggaagca ttgccgccgg agttgcgacc
     5041 gcccgcgaca ctgggaatgc ccccgaggcc ctcggggtag cgcgtggagc ttccggcgcg
     5101 tcccatcgtt ccaccgaccc agccaatacc gccggttgcg ttcgctcccg cagaattccc
     5161 ttctggcaaa ctcgagagct cggccgcgct gcgtgagccg cacgacgcga gcaacgccat
     5221 cgtgactagc cacgggccgt tcgagccgtt ccgatgaagc ccgttcgcac gctggccgcg
     5281 cttacgcatg acccacccaa ggtcttgggt ttggagattc gcgtcaagca ctcgctagat
     5341 tgatcagttg tttccgcctg acattacgcg atgaatgctc tcgggcacca agccgtcgtc
     5401 ctctgcttcg gccaccaagc gcgcgttg
//
LOCUS       DASTKU010001000         4734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1537966, whole genome shotgun sequence.
ACCESSION   DASTKU010001000 DASTKU010000000
VERSION     DASTKU010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537966"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VFQ61_35515"
     CDS             <1..832
                     /locus_tag="VFQ61_35515"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET9959872.1"
                     /translation="TTGSRSSGASETRERLLNAIKALAQRVPVNEITAADIAREADVS
                     WPTARRHLGDATQVRRWVLEVHPQAQESESTEQRLLKAAASVFATSGYAGATLEQVGQ
                     AAGLTKGAVYWCFKSKEELFLRVMKERDERLQLWGNALLSAELEANVASREFMPLLKR
                     VLEKHFRALAADETHLRLQIEFLAASRDATARRALAAAYRRQRQLIEGIVRALCTRGI
                     LREDAPVETIAQLLLLLFDGFVTMLLASPALAKDASWAAELARMLALGLEQYAPKPRA
                     "
     gene            1006..1539
                     /locus_tag="VFQ61_35520"
     CDS             1006..1539
                     /locus_tag="VFQ61_35520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959873.1"
                     /translation="MAFAGDIGGNAQSLPVPRSHELGVSLASVTPLAAVDSPSRAELG
                     LDYQHYFASPALRVGGGVRWQPGAPFTSWPVDLYADIACVFDGPMNFRPFVGLELGWT
                     GFNRLVVPEKDWPEGVVRAHQERLSKLYFGSVAAPLNFRHRSLTLSTLTLHLDALGYE
                     AHGLRLRVELLRVGGAW"
     gene            1536..2549
                     /locus_tag="VFQ61_35525"
     CDS             1536..2549
                     /locus_tag="VFQ61_35525"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET9959874.1"
                     /translation="MTSAVSWRFIVSTLGVLPLACSDPVTLDGFDASGTSRVTEQIES
                     ARIEHDVNQLYSSHRADRPIDCGSICDANPLVPSLSHDATRQLLRERLQLLRLPWTEQ
                     QLDQPVGLVNLYVDLPGATEPEEVVLVAAHYDAYFGGADDNSTGVAALLELARVLPSF
                     RFARTVRLAFFDLEELGCRGATLYTQRVALERHRIVFVLDSLGSRHTGAGSQPAEFGV
                     PWPSSADFLLVQANSAVARQAAQLELLNRTLRLTRLFTIVAPADGAYPLTTGLLNGDQ
                     IPFWLAHVPTVNFSDTPCCREHRMHTERDVPEALDPAFLTENTRFTAAAVAYFAGVLA
                     DAP"
     gene            2539..3099
                     /locus_tag="VFQ61_35530"
     CDS             2539..3099
                     /locus_tag="VFQ61_35530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959875.1"
                     /translation="MRRSSGSRRARVWRQRLLTLVVVVGALGAAPQASAKELRWFASS
                     HAAAALQLSEARTLGGAGGGIGAQAAWREFALLRLSTSFLMGSGAVHELALGIGVQRP
                     GAYCPAVWIGPRLLMGKGMGFITPDHLTPPSGPAWSFGVTLAPLRFQRANTQLTAIAP
                     YLGLGSDLPGSALAVGVTLLEVATAL"
     gene            complement(3183..4292)
                     /gene="ada"
                     /locus_tag="VFQ61_35535"
     CDS             complement(3183..4292)
                     /gene="ada"
                     /locus_tag="VFQ61_35535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HET9959876.1"
                     /translation="MARRTPAELSPHAPSASVSSAEIDAERWQALRRRDPALDGQFWY
                     AVSTTGIYCRPSCSSRLPRRENVQFYAAPQDAERAGFRACKRCKPSALSPAREHAALV
                     ARACQLIDQAEQLPPLEVLAAEVGLSRFHFHRVFKSVTSLTPKAYADAKRATRLQKAL
                     QNTDSSVTRAAYDAGFNSSSRFYTHAKQRLGMTPSTLRARGAGQRIHFAVAQCWLGAV
                     LIACSERGVCHVAIGDDPEQLLREFQERFAQAELKGADRDFEAVIARVLGCIELGELT
                     EELPLDVRGTAFQQRVWQALRAIPRGTTTTYSQIAEQLGQPAAVRAVAGACAANSLAV
                     LIPCHRVVRTDGSLSGYRWGIDRKRALLERESKSR"
BASE COUNT          840 a         1473 c         1499 g          922 t
ORIGIN      
        1 gacgaccggg tctagaagtt cgggcgcttc cgagacgcgc gaacgtcttc tgaacgccat
       61 caaggcgctc gcccagcgcg tgcctgtcaa tgagattacg gccgccgata tcgcgcgaga
      121 agcggacgtc agctggccca cggcgcggcg tcacttgggg gacgccaccc aagtccgacg
      181 ttgggtgctg gaggttcacc cccaagccca agagtcggaa tcgaccgaac aacggttgct
      241 caaagccgcc gctagtgtgt tcgcgacgag cggatacgcg ggagccacac tcgagcaagt
      301 ggggcaagcc gcgggcctga ccaagggggc agtgtattgg tgtttcaaga gcaaggaaga
      361 actgtttctt cgcgtgatga aggaacgcga cgaacgcctg cagctctggg gaaacgcact
      421 tttgtccgcc gagctcgaag ccaatgtcgc gagtcgcgag ttcatgccgc tcttgaagcg
      481 cgttttggag aagcacttcc gagccctggc cgcggatgaa actcacctac gtttgcagat
      541 cgagtttctg gccgcatctc gcgacgctac ggcacggcgc gcgctcgccg cagcgtatcg
      601 gcgtcaacgc cagctgatcg aggggatagt ccgagcactc tgcaccaggg gcatactgcg
      661 tgaggacgcg ccggtcgaaa ccatcgctca gctactattg ctgctgttcg atggtttcgt
      721 gacgatgctg ctcgcctcgc cagctctcgc caaagacgcg agctgggcgg ctgagctagc
      781 tcgaatgctg gcgctgggcc tggaacagta cgctccgaag ccacgcgcct gaggcctcgt
      841 cttgggcgga gatcagcggg taacggcgtg tcgcgcaccc aaatgttgac gcacatactt
      901 attagaatgt atatagactc aatgcgggtt tcggtggtgt tctggaaagg gtggagaagg
      961 tggtcgcgcc ccggttgcgc gaccgccctt tgcacttgga cttcggtggc ttttgccggt
     1021 gacattggcg ggaacgcgca gagcctgccc gttccgcgtt cccacgaatt gggcgtgagc
     1081 ctggcctcgg tgactccgct cgccgcggta gatagcccga gccgcgcgga gttggggctg
     1141 gattaccagc actactttgc atcgccggca ttgcgtgttg gcggcggagt gcgttggcag
     1201 cccggtgcac cctttacgag ctggcccgtg gatctctacg ccgacatagc gtgtgtcttc
     1261 gacggtccca tgaacttccg cccgtttgtc ggcctggagc tgggatggac cgggttcaat
     1321 cggctggtgg tgccggaaaa agactggccc gagggcgtcg tgcgcgccca ccaagagcgg
     1381 ctctccaaac tgtatttcgg cagtgtcgcc gcacccttga actttcggca tcgaagccta
     1441 accctgagca cgctgacgtt acacctcgat gcgttgggct atgaggctca tggtctgcgc
     1501 ctgcgtgtcg agctgttacg ggtgggaggt gcctggtgac ctccgcggtt tcgtggagat
     1561 tcattgtgtc cacgctgggt gtactcccgc tggcgtgttc ggatccggtg acgctcgatg
     1621 ggttcgacgc gagcgggacg tcccgagtga ccgagcaaat cgagtcggcc cgaatcgaac
     1681 acgacgtaaa tcagctgtat tcgagccaca gggctgatcg gcctatcgat tgcggctcca
     1741 tatgtgacgc aaatcctctc gtcccatcct tgagccacga cgcaactcgc cagctcttgc
     1801 gcgagcgttt acagctctta cgtctgccct ggaccgagca acagctcgat cagccggtgg
     1861 gactggtgaa tctatatgtc gatttaccgg gggccaccga acccgaggag gtggtgttgg
     1921 tcgccgccca ctacgatgct tacttcgggg gcgctgacga caactccacg ggcgtagcgg
     1981 ccttactcga gctcgcgcgt gtcttaccgt cgttccggtt tgctcggacc gttcgcctgg
     2041 ccttcttcga tttggaggag ctcggctgtc gcggcgcgac tctttatact cagagggtcg
     2101 cgctcgaacg tcaccggatc gtgttcgtgc tcgatagctt gggtagccgc cacaccggcg
     2161 cgggctcgca gcccgccgag ttcggcgtgc catggccgag cagcgcggat ttcctgctgg
     2221 ttcaagccaa ctcggcggtc gcccggcagg cggctcagct ggagctgctg aatcgaactc
     2281 tacggctcac acgcttgttt acgattgtcg cacccgcgga tggggcctat ccgctcacca
     2341 ctgggctcct caacggcgac caaatcccgt tttggcttgc tcacgtaccg accgtaaact
     2401 ttagcgacac gccttgttgt cgcgagcatc gcatgcacac ggaacgcgac gtgcccgagg
     2461 ccctcgatcc ggctttctta acggaaaata cgcgattcac ggccgccgcc gtggcgtact
     2521 tcgcaggagt tctggccgat gcgccgtagc tccgggagca ggcgggctag ggtctggcgg
     2581 cagcgtttgc tgacgctcgt cgtcgtggtc ggcgcgctcg gggccgcgcc gcaagcgagc
     2641 gcgaaggagc tgcgttggtt cgcgagttca cacgccgcgg ccgcgctcca gctgtccgaa
     2701 gcccgaactt tgggcggcgc gggcggcgga atcggcgcac aggcagcgtg gcgtgagttc
     2761 gcactgctac gcctgagtac gagctttctc atgggcagcg gcgcagtgca cgaactagcg
     2821 ctgggcattg gagtgcaacg gccaggagcc tactgcccgg ctgtttggat tgggccgcgc
     2881 ctgctgatgg gaaaaggcat gggcttcatt actcccgatc acctcacgcc accatccggt
     2941 cccgcttgga gctttggagt cacgttggct ccgttgcgct tccagagagc gaatacacag
     3001 ttgacggcaa tcgccccgta tctagggctg ggctcggact tgcctggcag cgcactggcg
     3061 gtgggcgtaa ccctactcga agtcgcgact gctctatgag agccatccca gcaattggga
     3121 atgggatctt ggagcttggt cgaacaatcg gaagagccac ctggcgtttg atcgattctc
     3181 tatcacctgg acttcgactc tcgctccaac aacgcccgtt tccggtcgat gccccaacgg
     3241 taaccggaga gcgagccgtc ggtacgaacc acccgatggc acgggatgag gaccgcgagc
     3301 gagttcgccg cgcatgcccc cgcgaccgct cgcacagcgg caggctgccc tagctgctcg
     3361 gcgatttggc tgtaggtcgt cgtggtgccg cgcgggatag cgcggagagc ttgccagaca
     3421 cgctgctgaa acgcagtgcc gcgtacgtcg agcggcaact cttccgttag ctcaccgagt
     3481 tcgatgcagc caagtacacg cgcaatcacc gcctcgaagt cgcgatctgc acctttcagt
     3541 tcggcctgcg cgaagcgctc ctgaaactcg cgtaacaatt gctccgggtc gtctccgatc
     3601 gcgacgtgac acaccccgcg ctcgctacat gcaatgagca ccgcgcctaa ccaacactgg
     3661 gccaccgcga agtgaatccg ctgtccggca ccgcgcgccc gcaatgtgct cggggtcatg
     3721 cccaggcgct gcttggcgtg cgtgtagaag cgactactgg agttgaagcc cgcgtcgtaa
     3781 gccgctcgcg tcacgctcga gtcggtgttt tgcaaagcct tctgcagtcg agtcgcgcgt
     3841 ttcgcatcag cgtaggcttt gggcgtcagg ctcgtgacgc tcttgaatac acggtggaag
     3901 tggaaccgac ttagtccgac ttcagccgcc agcacctcga gcggcggcag ctgttcggct
     3961 tggtcgatga gctgacaggc acgggccacc aaggcggcgt gctctcgtgc gggagacagc
     4021 gccgacggtt tgcagcgttt acatgcgcga aacccggccc tttccgcgtc ctggggcgca
     4081 gcgtagaact gcacgttctc ccggcgcggc aagcgcgacg agcacgaggg gcggcaataa
     4141 atgccggtcg tgctcaccgc gtaccaaaac tgtccgtcca gtgcgggatc ccgacggcgc
     4201 agtgcctgcc agcgctccgc gtcgatttca gcgctcgaca cggaagccga cggcgcgtga
     4261 ggcgacaatt cagccggggt tctgcgtgcc attcagaaac cctaatgtag ctttggcacg
     4321 cgggcgctcc gcttcttgct ccgcaattcg cgccccttgc ccgtagaaag gggatttggc
     4381 catagagctg gcacttcgcc cggtgcgtgg cgagtctgtg cgcgaccgcg gccttccacc
     4441 ccaattcgca cacaaactgc tgacagcacc ctgctgacag ccctgcgggc agcacctgcc
     4501 agccgccctg cctgggaacg gttccggtcc actctaacga tagctcacac ccgcagggat
     4561 tgtaggttga cgtatggcac gaagctgatc ctcgtcacag tcgcagaaca aatttcatgg
     4621 tacggagaag cccatggaaa cgcggcgagc cgctcgctcc ccagagcacg ccattgaaga
     4681 acctagaaag cgagaatcaa cctgggagcc tcgtcactca agtctctcgg attc
//
LOCUS       DASTKU010001001         6598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1314550, whole genome shotgun sequence.
ACCESSION   DASTKU010001001 DASTKU010000000
VERSION     DASTKU010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314550"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFQ61_35540"
     CDS             <1..665
                     /locus_tag="VFQ61_35540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959877.1"
                     /translation="EAGFTGTCGGDCEGKCDGQNSSGKCDGKCEGSCSANANGSCMAD
                     CTGEFKGTCGGQCKGGCTASGSVMCQGTCKGKCSYTPPDAKCNGECHGECKAEVSPPR
                     CTGTLNCQAGECKGSCEAKAEAKIDCSKPTVAVVVEGDPKLQAAFEANADAWGKASLE
                     VTKLGPVIEKVFTTGKASASAFGDVGVAGASCLVSAAEATVSASVSIKVSASASASVS
                     SS"
     gene            784..1755
                     /gene="corA"
                     /locus_tag="VFQ61_35545"
     CDS             784..1755
                     /gene="corA"
                     /locus_tag="VFQ61_35545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET9959878.1"
                     /translation="MATVLCLDGERGHIRKLTLTDREGLLAQTGPVWIDAQGNEPGLT
                     EFMRDELKLHPLAVEDIFESQQLPKIEDYGDYLYIVAHAVARSDSEGPFDFELVEIDL
                     VLTPKWVFSHHNGPFQAAGELVRELERNPRAIERGPAFVAHALLDRLVDEYLPVIDAV
                     DEEVDAIETAVIERPTPEVLERLFRLKRILQRLRRTAVHQRELLQRLARGEFEVIPEK
                     SLPFFRDIYDQFLRISDLADSYRELLSNALDAYLSVVSNRMNEVMKTLTIVATLMMPM
                     TFIVGVYGMNFVHMPELHWRYGYAYVWVLLIAVFVGMLGWFRYRRWL"
     gene            complement(1782..3041)
                     /locus_tag="VFQ61_35550"
     CDS             complement(1782..3041)
                     /locus_tag="VFQ61_35550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959879.1"
                     /translation="MTLAQGVVDVLCQYCGHVIHVEWNRPVPRPEHTRALTVYVPPAS
                     AARVPIFFVAGAAAVMAVGGIAFSLLLFRGASDANNDVVGGALENVKRGLASAPSFPV
                     HCGLNGSVEIVGQTFSGEGTLIEGEVNCKIRIRDSKLKGDVVVDAKNLVEVTVENSTL
                     EGKQAAIQSRMNSKIRLTKQSQLRAQEAAILAGLNTEISLRDSQISGDEQGIKAEHNV
                     RIEAFSSKISGNETGILGSLNAKISGRDVEVSGGRSGIEAQHNLELSLEGGKVEGKEA
                     GVRCKGSNAELKLTRKARIASPEVAVSGDNNLHLEMRDAQIDGGEIGVQASSNPKLAL
                     LAGARVHGGRVAIKAGLNLELEMREATLASEGVALCAPFNIEVSARTSTISGRDAFRF
                     ERPSDKLELEGTSVTGARVFNAKSCNP"
     gene            complement(3298..5145)
                     /locus_tag="VFQ61_35555"
     CDS             complement(3298..5145)
                     /locus_tag="VFQ61_35555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9959880.1"
                     /translation="MTTATVEPPARPAADEKRKRTEKILRAFHEEERFGRAYDGALLK
                     RLWAFLYPHRKLIWISMGVVLFTSAGSLLRPLLMRHVVDSGVMAKNESALLQGGLMLT
                     GLILFEQILGFVQLYATQVAGARAMADLRLHVFRFLHGLRLGFFDNQLVGRLVSRVTN
                     DIDAILDLFGSGALLAIGDLVKLVGIVSLMLVLDWKLSLIAFAATPPVMLLIVFVRRR
                     MREAFRDIRTKTAQMNSTMNEQVTGMTVIQAYSRQPAAAREFDTVNVDYRNANLQSIL
                     WDALQDAAIDTVSAVCLASIVVSLGYRPVSFGTVVAFSAYITQFFEPITTLAQRYTLL
                     QSAMAGAERVFKLLDTEAPDAPVGSALPPSNPEQAFSFENVNFAYKTDIPVLEHVTFS
                     ARRGEKVALVGPTGSGKTTVTALLLRLYDANSGVIRVDGDDVRGLERDKLRSRFAVVP
                     QDVVLFPGTVADNIAASEQPDRNRVESVLRRIGAYDLFERRSGGIDARVDENGANFSG
                     GERQLIAFARALYRDAPILILDEATASIDSDTESRLQHALEELMRGRTAIIIAHRLST
                     VKAADRIIVLQKGHIVEQGTHEELLQHAGLYARLHELQFSRAADAKLLA"
     gene            complement(5142..>6598)
                     /locus_tag="VFQ61_35560"
     CDS             complement(5142..>6598)
                     /locus_tag="VFQ61_35560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9959881.1"
                     /translation="MSTGEIMSRVTNDLAQVRLLLGFGVLNVINTVFALISALMVVIP
                     ISGRLTLAALTPLPLLLLVTRKFSHQFYTRTRDNQDSLGRMSARVQSSIAGVRVIRSF
                     ALENQEVAAFDKQNQDYLERNLKLAYLRGAFGLVLQATIAVGVIVVFWYGGHLVVTGS
                     LDAGGFLAFYRALGRLTWPLISMGFVMSLVQRGRASFSRLNDVYNAEPDVVDGKLALP
                     PAAEGSLSVRHLSYRHGDRDVLSDVSFDLPRGGSIAIVGRTGGGKSTLALLLARLLPT
                     PKQTVFLDGVDVCDLPLSTLRHAVGYAQQNAFLFSTTAGRNIGFPLEDPDSHEAHDKI
                     VRAAEDAQILPEVLSLPDGFDTVVGERGVQLSGGQKQRIALSAAFVSEPKILVLDDPL
                     SAVDARTERGILEALERKRRERSVILITHRVAAAALCDRILVLEQGKIVESGTHEELC
                     QQGGLYAAFAEEQRIESELERLGSADLPAEAVAS"
BASE COUNT         1244 a         2113 c         2040 g         1201 t
ORIGIN      
        1 gtgaagcagg gtttacaggc acctgcggcg gcgattgcga aggcaagtgc gacggacaaa
       61 acagcagtgg caaatgcgat ggcaagtgcg aaggcagttg ctccgcgaac gcgaacggca
      121 gctgtatggc ggattgcacc ggtgagttca agggtacttg cggcggccag tgcaaaggtg
      181 gctgcactgc ttccggctcc gtcatgtgcc aaggcacctg caagggcaag tgctcgtaca
      241 cgccgccaga cgccaagtgc aatggtgagt gccacggcga gtgcaaggcc gaagtatctc
      301 ccccccgctg cacgggcaca ctcaattgcc aagccggtga gtgcaagggc agctgcgagg
      361 ccaaagctga agcgaagatc gattgctcga agccgaccgt cgcggtcgta gtcgagggtg
      421 accccaagct gcaagcggcg ttcgaagcca acgcggacgc ctggggcaag gcttcgctcg
      481 aggtaaccaa gctcggtccg gtgatcgaga aggtattcac caccggtaag gcttctgcgt
      541 ctgcctttgg agacgtcgga gtggctggag cgtcgtgcct cgtgtctgcg gcagaagcca
      601 cggtcagcgc gtcggttagc atcaaagttt cagcctctgc aagcgccagc gtgagcagct
      661 cctaaacttg ttcgatagat aagatcgcgt gccgctcgtc ctcagacgtg cggcacgctg
      721 cgttttgtgc ccatctgtca gggaaagctg cagcgccgcc tgcactcgag ttacgctctc
      781 gcggtggcaa ccgtactctg cctcgatggt gaacgcggac atatccgcaa gctgactttg
      841 accgatcggg aggggctgct cgcacagacc ggtccggtct ggatcgacgc gcaggggaat
      901 gaacccgggc tgaccgaatt tatgcgcgac gagctgaagc tgcacccgct agcggtggaa
      961 gacatcttcg agtcgcagca gctgccgaag atcgaggact atggggacta cctgtacatc
     1021 gtggcccacg cggtggcgcg ctcggactcg gagggtccgt tcgatttcga gctggtcgag
     1081 atcgatctgg tgctgacgcc caagtgggtg ttctcgcatc acaacgggcc atttcaagct
     1141 gccggcgagc tagttcgaga gctggagcga aacccgcgcg ccatcgagcg cggtcccgcc
     1201 ttcgtggcgc acgcgctgct cgatcgcttg gtcgacgagt atctgcccgt gatcgacgcc
     1261 gtcgacgaag aggtggacgc gatcgagacg gccgtcatcg agcgacccac gcccgaagtg
     1321 ctcgaacggc tgtttcgtct gaagcgcatc ctgcaacgtt tacggcgcac ggccgtacat
     1381 caacgcgagc tcttacaacg cctggcgcgc ggtgaattcg aggtcattcc cgagaaatcg
     1441 ctgccgttct ttcgggacat ttacgaccag ttcttgcgca tctcggactt ggcggacagc
     1501 taccgcgagc tcttgtccaa cgcgctcgat gcctatttgt ccgtggtgtc gaatcgcatg
     1561 aacgaggtga tgaagacgct caccatcgtg gccacgctca tgatgccgat gaccttcatc
     1621 gtcggcgtct acggcatgaa cttcgtgcac atgcccgagc tgcattggcg ctacggttat
     1681 gcctacgtct gggtcctgct catcgccgtg tttgtcggca tgctcggttg gttccgttac
     1741 cgacgttggc tctgacgcga atttgccggt tcggcccgca actaaggatt gcagctcttc
     1801 gcgttgaaca cccgcgcgcc agtcacgctg gtgccctcga gctccagctt gtcactcggg
     1861 cgctcgaatc gaaaggcatc gcgacccgag atggtcgagg tgcgggcgct gacttcgatg
     1921 ttgaaaggtg cacacagcgc gacgccttca ctcgccagcg tagcttcgcg catctcgagt
     1981 tcgaggttta ggccagcctt gatcgcaacg cgaccgccgt ggacgcgcgc accggccaag
     2041 agcgcgagtt tggggttgct ggatgcttgc acgccaatct ctccaccatc gatctgcgca
     2101 tcgcgcattt ccaggtggag attgttgtcc ccggaaacgg ccacttcggg ggatgcaatg
     2161 cgcgcctttc gggtgagctt cagctcggcg ttcgagccct tgcagcgtac gccggcctct
     2221 ttgccttcga ctttgccgcc ctcgagcgaa agctccaaat tgtgctgcgc ttcgatcccg
     2281 ctgcgtccac cgctgacctc cacgtcacga ccggaaatct tggcgttcaa gctgccgagg
     2341 atgcccgtct cgttgccgct gatcttgctc gagaaagcct cgatacgcac gttgtgctcg
     2401 gccttgatgc cctgctcgtc gccgctgatt tgggagtcac gcaacgagat ttcggtgttc
     2461 aaaccggcca agatcgcggc ttcctgcgcg cgcagctggc tctgtttggt caggcggatc
     2521 ttggagttca tgcggctctg aatggccgcc tgcttgccct cgagggtgct gttctcgacc
     2581 gtgacttcga ccaggttctt ggcgtccacg acgacatcgc ccttcagctt gctgtcgcga
     2641 atgcggatct tgcagttcac ctcgccttcg atcagcgtgc cctcgccgct gaaggtttgg
     2701 cccacgatct cgacgctacc attgagcccg cagtgaacgg gaaagctggg ggccgaggcc
     2761 aagccgcgtt tcacgttctc gagcgcgccg cccacgacat cgttgttggc gtcgctagcg
     2821 cctcgaaata agagcagcga gaaggcgatg ccaccgaccg ccatcacggc tgccgcacca
     2881 gcgacgaaga agatcgggac tcgcgcggcc gaagcgggcg ggacgtaaac ggttagggcg
     2941 cgcgtgtgct cggggcgtgg caccggccgg ttccactcca cgtgaatcac gtgaccgcag
     3001 tattggcaga gtacgtcgac gacgccctgc gcgagagtca acggcgcacc acagttaggg
     3061 cactttttgg tcgtgagctt ggccatatcg agggctcgtc ggcggcgccg ccgacgcaac
     3121 cctggttcta acaacaaacc tgcgagcccg cgataggcca acgcacgcct acacccggca
     3181 cgtgacgcgc acgtagtatg acggttgacg aaggcgccga gctaagatgc gctcgggctt
     3241 ggcaaacctc ggatttacgc cgcggaacga ggatcacgga gcgccggaag ctcgcgctca
     3301 agccaggagc ttcgcgtccg cggcgcggct gaactggagc tcgtgcaggc gagcgtacaa
     3361 gcccgcgtgc tggagcagct cctcgtgggt gccttgctcc acgatatggc ccttctgaag
     3421 cacgatgatg cggtccgccg ctttaaccgt gctcaggcga tgggcgatga tgatggcggt
     3481 gcgcccgcgc atcagctctt ccagcgcgtg ctggagccgt gactcggtat cactgtcgat
     3541 actggccgtc gcttcgtcca aaatcaggat cggtgcgtcg cgatagagcg cgcgtgcgaa
     3601 cgcaatcagc tgccgctcgc caccggagaa attcgcgccg ttttcgtcga cgcgagcgtc
     3661 gatgccaccg ctccggcgtt cgaacaagtc gtaggcgcca atgcgacgta agacgctctc
     3721 cacgcggttg cgatcgggct gctcactcgc ggcgatgttg tccgcgacgg tgcccgggaa
     3781 caacaccacg tcctgaggca ccacggcgaa gcgactgcgc agtttgtcgc gctcgagccc
     3841 ccgcacgtcg tctccgtcca cgcgaatcac gcccgagttg gcgtcgtaca agcgcagcag
     3901 cagcgccgtc accgtggtct tgccggagcc agtcggcccc accagcgcga ccttctcgcc
     3961 gcgccgcgcc gaaaacgtga cgtgctccag aaccggaata tccgtcttgt aagcaaaatt
     4021 cacgttttcg aacgaaaacg cctgctcggg gttgctcggg ggcagcgcgc tgcccactgg
     4081 tgcatcggga gcctcggtgt cgagcagctt gaacacgcgc tccgcacccg ccatcgcgct
     4141 ctgcagcaac gtgtagcgct gagccagcgt cgtgatcggc tcgaagaact gcgtgatgta
     4201 cgccgagaag gccaccaccg taccgaagct caccggccgg taaccgagcg agaccacgat
     4261 tgaggccaag cacaccgcag acacggtgtc gatggccgcg tcttgcagcg cgtcccacaa
     4321 gatcgattgg aggttcgcgt tgcggtagtc gacgttgacc gtatcgaact cgcgagccgc
     4381 cgccggttgc cggctatagg cctgaatcac ggtcatgccc gtgacctgct cgttcatggt
     4441 cgagttcatt tgcgcggtct tggtgcgaat gtcgcggaaa gcctcgcgca tgcgccgccg
     4501 cacgaacacg atcagcaaca tcactggcgg tgtcgccgcg aacgcgatca gcgacaattt
     4561 ccagtcgagc accagcatca ggctcacgat gccgaccagc ttcaccaagt ccccgatcgc
     4621 gagtagcgcg ccggatccga acaaatccaa gatcgcgtcg atgtcgttgg tcaccctcga
     4681 gaccaagcgc ccgaccagct ggttatcgaa gaatcccagc cgcagaccgt gcaagaagcg
     4741 gaacacgtgc agccgcagat ccgccatcgc gcgcgcgccc gcgacttgcg tggcgtacag
     4801 ctgcacgaag ccgaggatct gctcgaacag gatcagcccg gtgagcatca gcccaccttg
     4861 gagcagcgcg ctctcgttct tggccatcac ccccgagtcc accacgtggc gcatcaggag
     4921 cggcctgagc aaagagcctg cggacgtgaa caacaccacg cccatcgaga tccaaatcag
     4981 cttgcgatga gggtagagga aggcccagag ccgcttgagc aacgctccgt cataggcgcg
     5041 gccaaagcgc tcctcctcgt ggaaggcgcg caagatcttc tccgtgcgct tgcgcttctc
     5101 gtcagcggcc gggcgcgccg gaggttctac ggtggcggtg gtcacgatgc tactgcctct
     5161 gccgggagat ctgcgctgcc cagccgctcg agttcgcttt caatccgctg ttcctcggcg
     5221 aaggccgcat atagcccgcc ttgctgacac agctcttcgt gagtgccact ctccacgatt
     5281 ttgccctgct ccagaaccag aattctgtcg cacagcgcgg cggcggccac gcgatgcgta
     5341 atgaggatga ccgaacgctc gcgacgtttg cgctcgagcg cctcgagaat gccgcgctcg
     5401 gtgcgcgcgt ccaccgcgct cagcggatca tcgagcacca gaatcttggg ctccgacacg
     5461 aacgcggccg atagcgcgat ccgttgcttt tgcccacccg agagctgcac tccgcgctcg
     5521 cccaccaccg tatcgaagcc gtctgggagc gacagcactt cgggcaggat ctgcgcgtct
     5581 tcggcggccc gcacgatctt gtcgtgcgcc tcgtggctgt ccggatcttc gagcgggaac
     5641 ccgatgttcc gcccagccgt ggtcgagaac aagaacgcat tctgctgcgc gtagcctacg
     5701 gcatgacgca aggtggagag cggaagatcg cagacgtcca ctccgtccaa gaacacggtc
     5761 tgcttcggcg tcggcagcag gcgcgcgagc aacaaggcca gagtgctctt cccgcctccg
     5821 gtgcgcccaa cgattgcgat cgatccgcca cgcggcaagt cgaagctcac gtccgaaagc
     5881 acatcgcgat ccccgtggcg gtacgacaga tgacgcacgc tgaggctgcc ctcggccgca
     5941 ggcggcaacg cgagcttgcc gtcgaccaca tctggctccg cgttgtaaac atcgttcagc
     6001 cgcgagaacg acgcgcgccc gcgctgaacg aggctcatca cgaagcccat cgagatcagc
     6061 ggccaagtca agcgcccgag ggctcgataa aacgcgagaa atccgcccgc atccagcgaa
     6121 ccggtgacga ccaagtgacc gccgtaccaa aacaccacga tcacgcccac ggcgatggtg
     6181 gcttgcagca cgagcccgaa agcacccctc aggtaagcga gcttgaggtt gcgctccagg
     6241 tagtcttggt tctgtttgtc gaacgcggcg acctcttggt tctccagcgc gaagctgcgg
     6301 atcacgcgta cgcccgcgat gctcgactgc acgcgggcgc tcatgcgccc caagctgtct
     6361 tggttatccc gggtgcgggt atagaattgg tgcgaaaact tccgcgtcac caggagcaac
     6421 aaaggcaagg gcgtcagcgc cgccaacgtg aggcgccccg agatcgggat cactaccatg
     6481 agcgcgctga tcagcgcgaa caccgtgttg atcacattca ggacgccgaa cccgagcagc
     6541 agacgaacct gggccaagtc gttggtgact cggctcatga tctcgcccgt gctcatac
//
LOCUS       DASTKU010001002         3501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1314684, whole genome shotgun sequence.
ACCESSION   DASTKU010001002 DASTKU010000000
VERSION     DASTKU010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314684"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VFQ61_35565"
     CDS             <1..662
                     /locus_tag="VFQ61_35565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959882.1"
                     /translation="WVFVLGVATLTPALVYAASAPDQRPFLGRCGQLKQDTPKGGELV
                     TLRGARAVQAALVFEDPLCPTCKAFHERLVTEGVFDKLDLRLALFPLDSECNWMLKDP
                     LHPGACLVSKAILCAGDQARTVLEWAYENQDELTQAGKTSAAALRARIRQRFGDSIDR
                     CIGDRKTEVRLNAHLHFASDNAIPVSTPQLYLGQQRVCDEDTDIGLRFTLAQLAPALL
                     K"
     gene            671..1396
                     /locus_tag="VFQ61_35570"
     CDS             671..1396
                     /locus_tag="VFQ61_35570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959883.1"
                     /translation="MDHATDEKKLPVALPALVGGAATLLLLAGSALAVRGWVSSAGQR
                     LVTPEAERPKVAIADHAEVAYCTPRFKEVLERVLHSCGLATDETRRGCKPSDVKTFAS
                     ISDDDFNELFTPLKDRGAVVQFDEGMDDLDENAKKLIEEKWFARKGARYFFVVARASK
                     SGTQAKNRALSHRRANSVLYYLKGIAQDPEIEKKVGLLWLGNEFAQLSREYCTWQTSR
                     SDKKCDDEAINRSAFVSWVDCRL"
     gene            1375..2091
                     /locus_tag="VFQ61_35575"
     CDS             1375..2091
                     /locus_tag="VFQ61_35575"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET9959884.1"
                     /translation="MGRLPSLKQGAGLLAWLGLLSAGGFAGGCDEAPRAAASAPRERS
                     QAVVAEAEPARPAVSVPAASAPSAAVRPKAPRALCAGQSAETKTLPKKRISQARASGA
                     TTKLPEHLSANGAFTWVNFWAAWCAPCKEEIPRLLKWERDLTQRGRRFSVRFISLDDD
                     ERQLRQFLESGQTGLNATYWLKEGKERDEWMAAAGIGSDPELPIHLLIDASGHVRCRV
                     QGAVEDADFAEVERLTAESH"
     gene            3177..>3501
                     /locus_tag="VFQ61_35580"
     CDS             3177..>3501
                     /locus_tag="VFQ61_35580"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET9959885.1"
                     /translation="MSKSTVSRVINGGYASAEVRARVAKVIQKLSYTPWTTARNFSLG
                     RAGSFGLVVWNTQSEWITQILGGIEEELSSKHISLLVGSLVLRGTYDTSTVSSWIRER
                     RIDALI"
BASE COUNT          765 a         1000 c         1093 g          643 t
ORIGIN      
        1 tctgggtgtt cgttctcggc gtagctaccc tgacgcccgc actcgtttac gcggcttcag
       61 ccccggatca gcggccattc ctcggccgtt gtggacagtt gaagcaagac acccccaagg
      121 gtggagagct cgtcacgctg cgtggagcgc gtgcggttca ggcggcgttg gtcttcgagg
      181 atccgttgtg cccgacttgc aaagcgttcc acgaacggct cgtgaccgag ggggtgttcg
      241 ataagctgga cctcaggctt gcgctctttc cgctggatag cgagtgcaat tggatgctga
      301 aagacccgct ccacccgggc gcctgtttgg tatcgaaggc gatcctatgc gcgggagatc
      361 aagcccgaac ggtgctcgag tgggcgtatg aaaatcagga cgagctaacc caagcaggca
      421 agacatcggc ggccgctctg cgcgcgcgca ttcgacagcg cttcggtgac tcgatcgatc
      481 gttgtatcgg ggatcgcaag accgaggtgc gcttgaacgc gcacctgcat ttcgcgtccg
      541 acaacgccat tccggtttct acgccacagc tctacttggg ccagcagcgg gtatgtgacg
      601 aggacacgga cattggactg cgcttcacgc tcgcccaact cgccccggca cttctgaaat
      661 gagcgaaaac atggaccacg caacagatga gaagaaactg ccggtcgcgt tgccggctct
      721 cgtcggcgga gcagctactt tgcttctgct ggcgggtagc gcgctggcgg tgcgcggttg
      781 ggtgagctcg gcagggcagc gcctcgtcac gcccgaggcc gaaagaccta aggtcgccat
      841 cgcggaccac gcggaagtgg cctactgtac cccacgattc aaagaagtgt tggagcgggt
      901 gctccactcc tgcgggctcg caaccgacga gactcggcgt ggctgcaagc cctccgacgt
      961 caagaccttc gccagcatca gcgacgatga cttcaatgaa ctattcacac cgctcaaaga
     1021 tcgcggcgcc gtggtccagt tcgatgaggg catggacgat ctcgacgaaa acgcgaagaa
     1081 gctgatcgaa gaaaagtggt tcgctcgcaa gggcgcccgc tacttcttcg tggtcgcgcg
     1141 cgcctccaag tctggcactc aggcgaagaa tcgggcgctt tcacaccggc gcgcgaactc
     1201 cgttttgtat tatctgaagg ggatcgcgca ggatccagaa atcgaaaaga aggtgggcct
     1261 gctttggctt ggcaacgaat tcgctcagtt gtcccgcgag tactgcactt ggcagacttc
     1321 ccgttcggac aagaagtgtg atgacgaggc tatcaatcgg agtgctttcg tatcatgggt
     1381 cgattgccgt ctttgaaaca gggtgcgggt cttctcgcgt ggctggggct cctgtccgcg
     1441 ggcggctttg cggggggatg cgacgaggcc ccgcgcgccg ccgcctccgc tccacgtgaa
     1501 cgcagccaag ccgtggtggc cgaggcggaa ccggcgcgcc ccgctgtgag tgtgccggct
     1561 gcttccgctc cttcggcggc agtgcggccc aaagctccgc gcgcgctgtg tgcgggccag
     1621 tccgcagaaa ccaagactct gccgaagaaa cgcatcagtc aggccagagc ctcgggggca
     1681 accactaagt tgcccgagca tctgagcgcg aatggggcct tcacctgggt caacttctgg
     1741 gcggcctggt gtgctccctg caaagaggag atcccgcgac tgttgaagtg ggaacgtgac
     1801 ctaacgcaac gagggaggcg cttcagtgtc cgattcatct cgctggatga cgacgaacgt
     1861 caacttcgcc agtttttgga gtccggacag accgggctga acgccacgta ttggctcaaa
     1921 gaaggcaaag agcgggatga atggatggcc gcggctggaa tcggttccga tcctgaactg
     1981 ccgattcatc tgttgattga cgcgagtggc catgtgcgtt gccgagtgca gggggccgtg
     2041 gaagacgctg actttgccga ggtggaaagg ctcaccgcgg agtctcattg agacatcagg
     2101 ggggggcgca ccggtaactt tcgcgggcgt gcgtcacgga acacgctccg gaaaccgggc
     2161 atgccgaatg taagagggaa attgcgctat ctcgaaccga actgaaagga tcgttggata
     2221 cgctctcagt agagagtagg gctgtgagtg ggcaccgctc agcgagctcc aaacggactg
     2281 aggaaccgct cccaaactta gctgacccgt ttggcccacc cacagacaca gaatcatcca
     2341 caccctgtca tccgcatcct gtcatctgca tcctgtcatc cgcgtcctgt gcactgggag
     2401 ccgtacttaa gtaagatcgc ccggcatagc gcataggctt gcgaaggcga gcggagcgat
     2461 cgaaaacaca aagagggaaa cgcgagtaga gggagccccg actacggacc ggccgaggga
     2521 taacgaagga ctagagggag agagtgaggt cgctccgccc gacccaaccg agcctcgcga
     2581 attggagctt cgttacaaag caaagcagcg cgtacttaca gagcaccgag aagcagggcg
     2641 tagtcacgag caccgagaag cagggcgcag ttaacggagc acccataagc agagcgtagt
     2701 cacgagcacc gagaagcagg gcgtagttta cggagcaccc ataagcagag cgtagtttac
     2761 ggagcacccc ataagtagag cgtagcgaga agcagagctc aggtatcgga cgcctcaccg
     2821 ggagcctcaa ctcgagggct tggcaaacca agaatgttct gccccattcg tactttggac
     2881 ccagtccgac gagagaacac aacaacaaaa agctcgtatc aacgcacgaa tcagcccatc
     2941 gaatcaatca cccattgcga gtccctctct gcgggtggat gcagccaagt gctgggagca
     3001 tcatccgcgc cttccgccga ccaccgagca ccgaccgtca atgtattacc cgtcgagttt
     3061 agaagcgtcg aaggtcgcga gaaacatgcg caggggcgaa ctagtagcgt ggtatgatgc
     3121 caggttaaaa aatggcccga agaaccgaca ttgctgaagt cgcgcgacat gcgcgcgtgt
     3181 cgaagagtac cgtctccagg gtaattaacg gtggctacgc gagcgcggag gtgcgtgctc
     3241 gcgtagcgaa agttatccag aaactcagtt atacgccgtg gacgactgca agaaattttt
     3301 cacttggccg cgccgggagc ttcgggctgg tcgtctggaa cacgcagagt gaatggatta
     3361 cccagatcct cggcggcatc gaagaggagc tcagttccaa gcatatttca ctgctcgtgg
     3421 gcagtttggt gcttcgcggt acttacgaca cgagcacggt ctcttcctgg attcgcgagc
     3481 gccgcatcga cgctctcata t
//
LOCUS       DASTKU010001003         4655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1985643, whole genome shotgun sequence.
ACCESSION   DASTKU010001003 DASTKU010000000
VERSION     DASTKU010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985643"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1089
                     /locus_tag="VFQ61_35585"
     CDS             79..1089
                     /locus_tag="VFQ61_35585"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET9959886.1"
                     /translation="MRGFRLDSVLRVGAFVALAQLTAEADAADANASVATAPQASGVP
                     AGAQPSPASELERTLNQVEREARDSERRFEELGKRMEAARLRSILRGRALTRQLRRGF
                     LPVGGGFEALVDHATRIERLRRGLAADVRQEHEAARERSVLAKQLQDLNARLGPLRSE
                     HRALARAETALLSEADRERAFQRAFMSSRGAGHAAVYGALGPSDPSDLARGFAGMQGR
                     LPFPIAGRTEVKRAGRSGSEGRGVELHARAGTPVRAVYPGRVAFADSYADYGKTVILD
                     HGSRYYTVSAHLGAIDVSVGDEVDSETRLGSVGDDGQGAFLYFEIRAGSDTVDASRWF
                     GL"
     gene            1280..2536
                     /locus_tag="VFQ61_35590"
     CDS             1280..2536
                     /locus_tag="VFQ61_35590"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HET9959887.1"
                     /translation="MAFRWFGISSWCLLAAACANNAGQSAPPVTPEQPATSAQAQTPA
                     APAAPAAPAAKAVVPPADDKNPFLGAEFYVNPEFKKNVEGSAAKGGKDAALMRKVAAY
                     PTAVWLDEIAKTKTIARHLDAAKAQQKTSGKPVVTVFVVYDLPNRDCSAKSSAGELKV
                     EANGEARYKSEFIDPIAAQFKAHPEQRIVAIIEPDSLGNLATNLNVPKCAASQDAYRN
                     SIAYAISQLALPNVSIYIDAAHGGWLGWDGNRENIAKIYKEVLSAAGGPDKIRGFATN
                     VSNYNVVSGDDGKRMEPSTPTPDELTYVAKLSQTLANLDINGKGFIIDTSRNGRGGIR
                     KKWGNWCNIKGAGLGERPTAKPAPGVDAYFWVKPPGDSDGTSDAKAARFDENCRSEDA
                     APDAPEAGRWFHNYFRDLVLNANPPL"
     gene            complement(2666..3982)
                     /locus_tag="VFQ61_35595"
     CDS             complement(2666..3982)
                     /locus_tag="VFQ61_35595"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9959888.1"
                     /translation="MSEHESSRKVHGPDESAQSSIGEAGAAFSAAAGPNLQGSLALEG
                     STENTASNSATTRPARAAKQATLPYVDGVRALCALFVLVAHSWFQPSNGFYAERWMNR
                     LGLSYAHLAVDAFIVVSGFVICLPIARQEDRIGSVTRFFQRRAKRILPPYYAALAFAA
                     VFGLVVAPHPTGTVWDNSLPVTWQKLLLHATLLQNMPSELPGGSVSYQFWSIAVEFQI
                     YLLVPFLVPLARRTSWALCAGAVFLVSLVVHHFLPTLDSIHHWYISLFAMGAAAARVC
                     VRQPERTSRWLAPGVGWLAIVLMVILVGGHANFTRRLVWIDGLVGVGVAAVLTGLYLD
                     PVNPKGRLERRLHAPLRRLLSSRVLAGIGVFSYSLYLVHPVLLHSIWMLVTWVGQLGG
                     AAKMHSVPLFWLILALNPLVVGAAWLFYRAFERPFLHRAPSREHTT"
     gene            complement(4239..>4655)
                     /locus_tag="VFQ61_35600"
     CDS             complement(4239..>4655)
                     /locus_tag="VFQ61_35600"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HET9959889.1"
                     /translation="EKLRLFVYGSLLAGERDHELLSGCECLGSFYTKPTYTLYDLGVF
                     PALVVSGDSRVLGELYVIDRKTRFEIDVRKQYPALFDRAEIELEDGTRAEAYVMQLEQ
                     VRGKRKIKHGSFRDRFSPVPRARSHAPIVDWARKRF"
BASE COUNT          912 a         1525 c         1461 g          757 t
ORIGIN      
        1 ggctctcggt ttggtcgcac tcggaggcct gctcggcgcg ggctcggcct tgttcagcct
       61 gcgccggctg ctcgcgttat gaggggtttt cggctggatt ccgtgttgcg cgtcggcgcc
      121 ttcgtcgcgc tggcacaact cacggccgag gccgatgcgg ccgacgccaa cgccagtgtt
      181 gcgacggctc cgcaagcatc cggagtgcct gccggcgccc agcccagccc cgcgagcgag
      241 ctcgagcgga ccctcaatca ggtcgaacgc gaggcgcggg acagcgagcg gcgcttcgaa
      301 gaactgggta agcgcatgga agcagctcgc ttgcgctcga ttctgcgcgg ccgtgcgctg
      361 acacggcagc ttcggcgcgg ctttttgccg gtgggtggtg ggttcgaagc cttggtcgat
      421 cacgcgactc gcatcgagcg tctgcgaaga ggtctcgccg ctgatgtgcg tcaggagcac
      481 gaggccgcac gcgagcgctc cgtgctggcc aaacaactgc aggacctgaa cgctcgcctg
      541 ggcccgttgc gcagcgagca tcgcgctctg gcccgcgccg aaaccgcgtt gctgtccgaa
      601 gcggatcgag agcgagcctt tcaacgcgcg ttcatgagct cacggggagc gggccacgct
      661 gccgtttacg gcgcgctcgg gccgagcgat ccgagcgatt tggcgcgcgg tttcgccggc
      721 atgcagggac gcctaccctt tccgatcgcg ggccgaaccg aagtcaagcg agccggccgc
      781 tctggcagcg aggggcgcgg cgtggagctg cacgctcgcg cgggaacgcc ggttcgcgcg
      841 gtatatcccg gccgggtagc gtttgcggac agctacgccg actacgggaa gaccgtgatc
      901 ttggatcatg gctcgcgcta ctacacggtc tcggcgcacc tcggcgcgat cgatgtctcc
      961 gtcggagacg aagtggacag cgagacccgt ctcggaagcg tcggggatga cgggcagggt
     1021 gctttcctct acttcgagat ccgcgccggc agcgacaccg tcgatgcgtc acgctggttc
     1081 ggcctctgag ccgcgtcatc ccccctttga cgccatcacg gcattctgcc tagcgtccgc
     1141 gacgcttcgc ctttttcgct cggagttcgc ggatcagcag ctcttctgcc ggcgtacccg
     1201 ccgcatgcaa gctcgcgacg ctgaccgccc tcgagacgaa gagccgacgc gaatcatcgt
     1261 tttcaaggag atagatgtga tggcttttcg gtggtttgga atttcctcgt ggtgcttgct
     1321 cgcggcagct tgtgcgaaca acgcgggcca gagcgcgccg cctgtcacgc ccgaacagcc
     1381 ggcgacctct gcgcaagcgc aaacgcccgc ggctcctgct gcgcccgcag ctcctgccgc
     1441 gaaggccgtc gtgccccccg cggacgacaa gaacccgttc ttgggtgcgg aattctacgt
     1501 taaccccgag ttcaagaaga acgtagaggg ctccgccgcc aagggcggca aggacgcggc
     1561 cctgatgcgc aaagtcgccg cttatcccac cgccgtttgg ctcgacgaga tcgccaagac
     1621 caagaccatt gcgcgccacc tcgacgcggc caaggctcaa cagaagacct cgggcaagcc
     1681 agtagtcacg gtgttcgtgg tctacgactt gccgaaccgc gactgctccg cgaagtcctc
     1741 agccggcgag ctgaaggtcg aagcgaacgg cgaggcccgc tacaagagcg agttcatcga
     1801 ccccatcgca gcgcaattca aggcacaccc cgagcagcgc atcgtcgcga ttatcgagcc
     1861 ggattcgctg ggcaacctcg cgaccaacct gaacgtaccg aaatgcgcgg cgtcccagga
     1921 tgcgtatcgc aactcgattg cttatgcaat ttctcagctc gcgctcccca acgtgtcgat
     1981 ctacatcgac gccgcgcacg ggggctggct gggttgggac ggcaatcgtg aaaacatcgc
     2041 caagatctac aaagaggtgc tgagcgccgc gggtgggccc gacaagatcc gcggtttcgc
     2101 taccaacgtc tcgaattaca acgtggtgag cggtgatgac ggcaagcgca tggaaccctc
     2161 cacccccacg ccggacgagt tgacctacgt agccaagcta tcgcagacgc tcgccaacct
     2221 cgacatcaac ggcaaaggtt tcatcatcga tacgagccgc aatggccgcg gcggcatccg
     2281 caagaagtgg gggaactggt gcaacatcaa gggcgcaggg ctcggagagc gcccgacggc
     2341 caaaccggcg cccggcgttg acgcatactt ctgggtcaag ccacctggtg actcggatgg
     2401 cacctcggac gcgaaggccg cgcgcttcga cgagaactgc cgatcagaag atgcggcccc
     2461 cgatgcaccc gaggccgggc gttggttcca caactacttc cgtgacttgg tgctgaacgc
     2521 gaacccgccg ctctgaccgg cgctctgccc atcgaaatcg taggcccgct tctcgtccga
     2581 gggcgggcct acttttttgg tacgcgaact tcgcggactg ccgctacgga cgcggagcgg
     2641 gtggttggcg agctccggag tagattcatg tcgtgtgctc ccgactagga gctcgatgca
     2701 agaatggccg ctcgaacgcg cgatagaaca gccaagccgc gccaaccacg agcgggttga
     2761 gcgccaaaat cagccagaac agcggaacgc tatgcatctt tgccgcgcca ccgagctgcc
     2821 cgacccaggt caccagcatc caaatcgagt ggagcagcac gggatgaacc aagtacaagc
     2881 tgtaagaaaa cacgccaatt ccggccaaaa cccggctcga caacaatcgc cgcaggggag
     2941 cgtgtagccg ccgttccaac ctacccttgg ggttgactgg atccaagtac aaccccgtca
     3001 gcaccgcggc cacgccaact ccgaccaagc cgtcgatcca aacgagccgc cgagtgaagt
     3061 tcgcgtgacc tcccacgagg atcaccatca gcacgatcgc gagccaaccc acgccgggag
     3121 ccagccagcg actcgtccgc tcgggctgac gcacgcagac tcgcgcggcg gcggctccca
     3181 tcgcgaacag gctgatgtac cagtgatgaa tactgtcgag cgtgggcaag aagtgatgca
     3241 cgacgagcga gaccaagaac accgcgcccg cgcagagagc ccagctcgtc cggcgcgcga
     3301 gcgggacgag aaacggtacc agcaagtaga tctggaactc caccgcgatg ctccagaatt
     3361 ggtacgagac gctaccgcca ggcagctcgc tcggcatgtt ctgaagcaac gtcgcgtgca
     3421 acagcagctt ctgccacgtc accggcaggc tgttgtccca gaccgtcccg gttgggtgcg
     3481 gcgccacgac caaaccgaag acggccgcga acgcgagcgc cgcataatac gggggcaaaa
     3541 tgcgcttcgc gcggcgctgg aagaagcgcg tgacggagcc gattcgatct tcctgcctgg
     3601 caatcggtag gcagattacg aagcccgata cgacgatgaa agcgtccacc gccaagtgcg
     3661 catacgagag ccccaatcga ttcatccaac gctcggcgta gaagccgttg ctcggctgga
     3721 accagctgtg cgccaccaac acgaacaggg cgcaaagcgc gcgaactccg tccacgtatg
     3781 gcagggtcgc ttgttttgca gcccgggcag gccgcgtcgt cgcggaattc gaagccgtgt
     3841 tttcggtgct gccctcgaga gcgagggagc cctgcagatt cggtccagcg gcggcgctga
     3901 aggccgcccc tgcttccccg atggagcttt gggcgctctc gtccggaccg tgcaccttac
     3961 gcgaggattc atgctcgctc atgtggctct catgttaaac gcgaagcgca ccgtgcgccc
     4021 aggaaaacga gcagtttccg gtacaagagc caaggcgcgc gcgcccgcgc ctgctcggaa
     4081 acacgcactc gaggagtagg gatccgtctc gaaaccgact caaaaccggg tggagcgcgg
     4141 accccgcgag tgagaatccc ctagggcaat gcggcatgcg ccggaacgcg cgcaagcacg
     4201 ccgcatgatg tgccaacgtt ccggtctgaa ccgctggttc agaaacgctt gcgggcccaa
     4261 tccacgatcg gtgcgtggct tcgagctcga ggtacgggac tgaagcgatc ccggaagctg
     4321 ccgtgtttga tcttgcgctt gccgcggact tgctcgagct gcatcacgta agcttctgcg
     4381 cgcgtgccgt cctcgagttc gatctcggcg cggtcgaaca gcgcggggta ctgcttacgg
     4441 acgtcgatct cgaagcgagt cttgcgatcg atcacgtaca actcccccag cacccgcgag
     4501 tccccactca ccaccagcgc cgggaacaca cctaggtcgt acagcgtgta tgtgggcttg
     4561 gtgtagaagc tacccaggca ctcacaaccc gataggagct catgatctcg ctcacccgcg
     4621 agcagagagc catagacgaa caaacgcagc ttttc
//
LOCUS       DASTKU010001004         9934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_419103, whole genome shotgun sequence.
ACCESSION   DASTKU010001004 DASTKU010000000
VERSION     DASTKU010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419103"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFQ61_35605"
     CDS             <1..244
                     /locus_tag="VFQ61_35605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959890.1"
                     /translation="ELEELSLESFDLTSLAEAGRDIEAWVVLAQHAVYAAIGRVVRGR
                     FQGVHAADACLADLLISRLRSNARRAADVRQDRALA"
     gene            241..1362
                     /locus_tag="VFQ61_35610"
     CDS             241..1362
                     /locus_tag="VFQ61_35610"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9959891.1"
                     /translation="MSSREASEFGEGAPAELGPELLELHGELVQTNLGELLRALFRAR
                     KSGVLNLSTKTGDGAIWLERGGIADASYRQFEGKKALFRLFGETDAVYSFVDSQSTDW
                     LRRLDADTETLVAEGLQALAHVRSKVSALSLDTDAFARTKLESKPTERPVPRDASSPP
                     SQPLPASELEGLLLAVLSAPRTLTELLDLIPALDVEIVDAVVKLLGDGTLRRLPNAAR
                     KIAGLEPETLARLIWRKQRPGFRGPPRIGVAASDAELAIIQLHLGRLIGAHLPIEPVS
                     ASGCPHLLATWELAEGVELELFGVPLNETYAPLWGVFFPGLLSLLVLTGTRPQALERA
                     CEAAALTWVDVHERVPSLEALDPSQLSVLIRQCVEAASE"
     gene            1355..2461
                     /locus_tag="VFQ61_35615"
     CDS             1355..2461
                     /locus_tag="VFQ61_35615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET9959892.1"
                     /translation="MNDASPSPPARNLRIACIPGDGVGPEVMRPALLLLEWLKSERGL
                     PLEIWPLDLGAERYLREGVGLPEALKAEIRATASAVLLGAVGDARVPDHAHAREILFG
                     LRFGFDLYANVRPVKALTDALVPLVGRRAADVDWVIFRENTEGLYAGVGGQLRRGTTH
                     EVAIEEDVNTRLGVERILRAAFEFARRTGRRLCMSDKSNALRHAHDLWQRVFQELRRE
                     FSDVEAEHLYIDALCYELVRDPARFSVMVSCNLFGDIVSDLAAALGGGLGLAPSANLC
                     PGGQVPGLFEPVHGSAPGLVGRGTANPIAMLRTVGLMLEELGYPGERAALERACAEAL
                     RAGEGTVDVGGGMDTNAAGAAVLRRFNENLRVGG"
     gene            2477..5968
                     /locus_tag="VFQ61_35620"
     CDS             2477..5968
                     /locus_tag="VFQ61_35620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9959893.1"
                     /translation="MPMSGVPTARDEDQAGILGARGEFIASLPRRIEGLRSALSALLE
                     LPADDGERQNALLRRLHALASSARVLEFASVADALTEAENALRRMRPGQPAPAVAEVS
                     RALDVLPSLVLGAPAERPTSIPDALRPPSGARTFPISVLVLGPPGLAHMLARADSAQF
                     EAEQTDDLVRARELLRVTGPDAALVDGDLRGAREFLEQLGRDPLVAPLPVVVVGDFRT
                     PDAATAFIAAGANRILPKPVNPETLARTLSELREAGERPRVAREPLGELSIDQLAERI
                     AEEFRRGLTESLDQGSAASRVQFGEGADVLGAIWGAVARVRELSTLRSQGSIRFKAVG
                     PEGAIPLAPWSSDQRRAGDRGGRAPSNDDDVRLKGRRVVVVDDDPAVVWFLSGLLKAV
                     GAEVIEAHDGDRALSKVFENHPDLVVSDVLMPGLDGFALCHEIKRDVAVRDVPVILLS
                     WKEDLLQRMRELGADADGYLQKEASASTVVERIREVLRPRARVEQRLAAGGEVRGRLD
                     GLTPRLLLELLGGAAPDSTLTIRDAVYLYEANLRGGRLKSVTRSSSEGSFERGPRVLA
                     GLLGVSAGRFVVQRDSSPCRGEFDAPLAELLAIPVRHARAALAAVSAASLAGLKHLKL
                     DGPAVRAYLTATPSSAAKLVERLLGGESPRELLLSGASSAGWLENVLSDLARRGAILE
                     FDVDSSIVPKASRQAESPRTEAPAAPSVARTEAPSQSAPEPTIEPPPRVSFPELSRPP
                     APSLEDWGKNPLESGDARKLPAVAAVSRPSRPELDDVNDLFADLTDPSADHFAPSQAD
                     SNPAAANQNMRSQGSDHAAVTRLGLGPDDPGRESSLPRPGIRPVLSIPEGPPKASPSM
                     ADVFAAALRESPNDSLAVESPVPPPVSDPVRTQTLQAVRLPERDESSAELIGAWSATP
                     PPPAPATEAAPTTLLLDNPSGARPAAGAPEGAAGRARAPDNQADGPEPAPIGKTLVSA
                     SLPEAKSDSERSSARRLAAKVPSSAPGSKPPSSKPSSKSSAKVGTEATDSSAGSGVRT
                     VILACVAFLTAFLLVNSVLVPLLAPKEAREPATTVAEPANPAPDSVVVTERLPMPAGV
                     TTTPGSGLLEVEFAATEELQVNGQVQGRGPVRMVPLPVGKHTVRVLRGDAGREVHIEV
                     SEGQRTRVSFDRASKSP"
     gene            6052..8253
                     /locus_tag="VFQ61_35625"
     CDS             6052..8253
                     /locus_tag="VFQ61_35625"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET9959894.1"
                     /translation="MASPSTPAAEIAEEAERYELTSRLDACDIQHDGALIDFGSHGTE
                     SRRHFAVGPFTDVAPIDREGASFQQVFSKALNVEVWLDQPLNKPSLSLRAHGGTARLV
                     HVSVDEQRLGALRLPNGETRVLATGAASTPLPRGRHRVTLRFSPAPRGSKLPLVELDW
                     LRLGELQAEPAKYAAPTYEDIVSDVALDRIPKHSLVLRAPSSVRCFLRPSADSRLKVA
                     VGLWGSGKGSAEIKLLRDAEPPVLLQSRKVTGGDGATWTPVTVDLGPYAGSIVGLELY
                     ANEATRGGRIAFGDPVLVRQTERPPVSARARVAVVVVLSALERRRVPPWGPTGSLATL
                     ADLARNATVFGSHRVPSSVPAAVLASLLSGLSPRAHGLEDPNSKLAPEIHLLSEAVKE
                     ANGRTAMFTGAPTSFATFGFDQGWDVFDPISPVKDYPASEPLQRATRWLEQALDDGLS
                     GPVLVVVHARGAHPPWDVTREESQHLKPQDYAGIVDPRRGGILIGALRSKPTQRNKVR
                     RMSDDDWARLRALSDVALAKQDAAIGQMVGMLKRKSAWDDTAFVVTSDVAPGDPPDFP
                     FDPRGPLTEDRLLVPLYVHFPGNNVGGQEARAASAMMDLNVTLLHLLGLRVPSSLPSV
                     DLLARALGREPIVVRSELATLNDKYSARLGSWLLRGQIGSVPTLCALDVDPACATDTF
                     DRELIAARALWQATFRELTDARKLTPKDEVRRVTTIDEDTAAALTVWGDHQ"
     gene            complement(8296..8994)
                     /locus_tag="VFQ61_35630"
     CDS             complement(8296..8994)
                     /locus_tag="VFQ61_35630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HET9959895.1"
                     /translation="MSAQDILKLSEIFSSVQGEGPSAGEPAVFLRLALCNLRCGFCDT
                     RYTWDFEQYDYEREVSTAPVESVLARVLSHSERRLIITGGEPIMQQRALSSLIAQLPS
                     DWQIEVETNGTLIPNASLLQRVDQWNVSPKLAHSEEPLERRMKPEVLRVLLATQRAYL
                     KLVVRDAGDREEVEAVVSALEWPKSRVFLMPEARTVPEHRGRGDHVAELARQLAVRYS
                     PRLHVLMFGGERGR"
     gene            9085..9852
                     /gene="rph"
                     /locus_tag="VFQ61_35635"
     CDS             9085..9852
                     /gene="rph"
                     /locus_tag="VFQ61_35635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET9959896.1"
                     /translation="MPSVSRTDRSSRALRPVDMQIGFHRLAEGSVLYRAGGTVVLCTA
                     SIDNSVPEFLAGKGQGWITAEYQMHPRANPKRRENRDGRERPLGGRTREIQRLIGRAL
                     RSAVDLSRLGERTLAVDCDVLEADGGTRTASITGGFVAIAAAMAKLEARGALRSPALR
                     DQVAAISVGHVGDEVLLDLCYTEDSAARVDLNLVATHSGAVVEIQGTAEGEAIPRSEL
                     DSMVDLALVGIQDLCRLQNDALAAAGIALDQLLIPRA"
BASE COUNT         1588 a         3171 c         3390 g         1785 t
ORIGIN      
        1 agagctcgag gagctatcgc tggagtcgtt cgacctcacg agcttggcgg aagccgggcg
       61 cgacatcgag gcctgggtag tcttggcgca gcacgcggtc tacgccgcga tcgggcgcgt
      121 ggtgcgcggg cgcttccaag gcgttcacgc ggccgacgca tgtctcgcgg atctcctcat
      181 ctcacgtctg cgctcgaacg cgcggcgcgc ggccgacgtg cgccaggacc gagcgttggc
      241 atgagctctc gagaagcttc ggagtttggc gagggggccc cggccgagct cggccccgag
      301 ctgctcgaac tacacgggga gctcgtccaa acgaacttgg gtgagctgct gcgcgccttg
      361 tttcgagcgc gtaagtcagg cgtcttgaac ctgagcacga agaccggtga cggtgcgatt
      421 tggctcgagc ggggtggcat cgctgacgca tcctatcgac agttcgaggg caaaaaggcg
      481 ctcttccgtt tgttcggaga gaccgacgcg gtttactcct tcgtcgactc gcagagcacg
      541 gattggctgc gccgcctcga cgcggacacc gagacgttgg ttgccgaagg cctccaggcg
      601 ttggcgcacg tgcgcagcaa agttagcgcc ttgagtttgg ataccgatgc tttcgcgcgc
      661 acgaagctcg agagcaaacc cacggagcgg cccgtgccgc gggacgcgag ctcgcctccg
      721 agccagccgc tgccggcgag cgagctggaa gggctattgc tcgcggtcct gtcggcaccg
      781 cgtactctga cggagctttt ggacctgatt cccgcgttgg acgtcgagat cgtggacgcc
      841 gtagtcaagt tgctcggaga cggcacgctc cggcgcctgc cgaacgccgc tcgcaaaatc
      901 gctgggctcg agcccgaaac gttggcgcga ctgatctggc gtaaacagcg ccctggattc
      961 agaggtccgc ctcggatcgg ggttgccgca agcgacgcgg agctggcgat aattcagctg
     1021 catctcggcc gtttgatcgg tgcacacttg ccgatcgagc ccgtgagtgc gtcggggtgc
     1081 ccccacttgc ttgcgacctg ggaactggcc gagggcgtcg agctggaact attcggcgtg
     1141 ccgctcaacg aaacttacgc tccgctctgg ggcgtgttct tccctggatt gctcagttta
     1201 ctcgtgttga ctggcaccag gccgcaggcc ctcgagcgcg cttgtgaggc cgcggcgctg
     1261 acgtgggtcg atgtccatga gcgggtccct tcgctggaag cactagaccc gagccaactt
     1321 tcggtcttga ttcgtcagtg tgtcgaagcc gcgagtgaat gacgctagcc cgagcccccc
     1381 ggctcgcaat ctccgtatcg cctgtatccc aggcgatggc gtgggacccg aagtcatgcg
     1441 accggcgttg ctgctgctcg agtggctgaa gtcggagcgg ggcctgccgc tcgagatttg
     1501 gccgctagat ctcggcgcag aacgatactt gcgcgagggg gttggcttgc ccgaggcgct
     1561 caaggccgag attcgcgcga ccgcgagcgc ggtattgttg ggtgccgtag gagacgcgcg
     1621 tgtgcctgac cacgcgcacg cccgcgagat cctgttcggg ctgcgcttcg gcttcgatct
     1681 ctacgccaac gtgcgccccg tgaaagcctt gacggatgcg ctcgtgccgc tcgtcgggcg
     1741 tcgcgccgcg gacgtggatt gggtaatctt tcgagagaat accgaaggtc tttacgctgg
     1801 cgttgggggc cagctgcggc gtggtacgac gcacgaggtt gcaatcgagg aggacgtcaa
     1861 cacacgtctt ggcgtggagc gaattttacg cgcggcgttc gagttcgctc ggcgtacggg
     1921 ccgtcgccta tgcatgtccg ataagtcgaa cgccctgcgc cacgcccacg acctgtggca
     1981 gcgcgtcttc caggagctcc gccgcgaatt ttcggacgtc gaagcggagc acctgtacat
     2041 cgacgcgctc tgttacgagc tcgtgcgcga ccccgcccgc ttctccgtga tggtgagctg
     2101 caacttgttc ggagatatcg tctctgacct cgcggcggcg cttggcggcg gcttggggct
     2161 cgcgccgagc gcaaatctct gccccggcgg tcaggttccg ggcctgttcg agccagtgca
     2221 tggttcggcc ccagggctag tcggtcgagg aacggcgaac cccatcgcga tgctgcgcac
     2281 cgtagggctg atgctcgagg agctcggcta tccgggtgag cgcgcagctt tggagcgggc
     2341 gtgcgccgag gcgcttcggg cgggtgaggg gactgtagac gtgggtggcg gtatggacac
     2401 caacgcggct ggcgcggccg tcctgcgaag attcaacgaa aatctccgcg tcggaggcta
     2461 gcctgtggta cgccccatgc ccatgtccgg ggtcccgacc gcccgcgacg aagatcaggc
     2521 cggaatcctc ggtgcgcgtg gcgaattcat tgcaagcctg ccgcgacgca tcgaagggct
     2581 tcggagtgct ctgagcgcgc ttctcgagct tccggccgat gacggagagc ggcagaacgc
     2641 gctgctccga cggctccatg cgctcgcgtc ttcggcacgc gttctggagt tcgcctccgt
     2701 tgcggacgcc ctcaccgaag cggaaaacgc gctgcgccgc atgcggccgg ggcaacccgc
     2761 acccgcggtg gcggaagttt cgcgcgcgct ggacgtactg ccttcgctcg tgctcggtgc
     2821 gccagcggag cgaccgacca gcattccgga tgcgctgcgg cctccgagcg gcgcgcggac
     2881 cttcccgatc agcgtattgg tgctggggcc gcccggcttg gcccacatgt tggcgcgggc
     2941 ggactcggcg cagttcgaag cagaacaaac ggatgattta gtgcgcgccc gtgagctgct
     3001 gcgcgtgacc ggtccggacg ccgcgctcgt agacggagat ttgcgggggg ctcgggagtt
     3061 cctggagcag ctcgggcgag acccgctggt ggcgccgctt ccggtggtgg tcgttgggga
     3121 cttccgcacg cccgacgcgg ccaccgcgtt cattgcggcg ggcgcgaacc gcatcttgcc
     3181 caagccggtg aacccggaga ctttggcgcg tacgctctcg gagctccgtg aagccgggga
     3241 gcgcccgcgc gtggcgcgtg agcctctggg cgagctcagc atcgaccagc tcgcggagcg
     3301 tatcgcggaa gagttccgac ggggtctgac ggagagcttg gatcaagggt ctgccgcttc
     3361 ccgggtgcag ttcggtgagg gcgcggacgt gctgggcgcg atttggggcg cggtcgcgcg
     3421 cgtgcgcgag ctctccacgt tgcgctctca gggctcgatt cgcttcaaag ccgtgggtcc
     3481 ggagggcgcc attccgctcg ccccctggtc ttcggatcag cgacgcgctg gcgatcgtgg
     3541 cgggcgcgcg ccgagtaacg acgacgacgt gcgcctgaag ggtcggcgtg tggtcgtggt
     3601 ggatgacgac ccggccgtcg tctggttctt gagcgggttg ttgaaggccg tgggcgccga
     3661 ggtgatcgaa gctcacgatg gcgatcgcgc tctcagcaag gtgttcgaga accacccgga
     3721 tttggtcgtc agtgacgttc tgatgcccgg cctggacggg ttcgcgttgt gtcacgagat
     3781 caagcgggac gtggcggtgc gcgacgtccc cgtcatcctc ttgtcctgga aagaggacct
     3841 gttgcagcgc atgcgcgagc tcggcgcgga cgccgacggc tatttgcaga aagaagcttc
     3901 ggcatcgacc gtcgtcgagc gcatccgaga agtgctgcgc ccacgcgccc gcgtcgagca
     3961 acgccttgcg gcgggtggtg aggtgcgagg ccgtttggac ggcttgacgc cgcggctgct
     4021 gctcgagttg ttgggcggcg cggcaccgga ttcgacgctg acgatccgcg acgcggttta
     4081 cctgtacgaa gccaatctgc gcggcggacg cctcaaatcg gtgacccgca gctcgagcga
     4141 aggcagcttc gaacgcgggc cacgtgtgct cgcagggctg ctgggggtga gcgcggggcg
     4201 cttcgtcgtc cagcgcgata gctctccctg tcgcggtgaa ttcgacgctc ctctcgcgga
     4261 gctgttggcc attccggtgc ggcatgcgcg cgcggcgctt gctgcggttt cggccgcaag
     4321 cctagccggt ctaaagcact tgaagctcga tgggccggcc gtgcgcgcat acttgaccgc
     4381 cacacccagc tcggccgcca agctcgtcga acgactgcta ggcggtgagt cgccacgaga
     4441 attgctgctc tccggcgcga gctccgcagg ctggctcgaa aatgtgctct cggacttggc
     4501 ccggcgtggc gcgattttgg agttcgatgt ggattcctcg attgtgccga aggcctcgcg
     4561 tcaagcggag tccccccgga cggaagcccc ggcggctcca tcggtagctc gaaccgaggc
     4621 accgtcgcaa tcggcacctg agcctacgat cgagcccccg ccgcgcgttt ctttcccaga
     4681 actttcccga cctccggctc cttcgttaga agactggggc aagaacccgt tggagagtgg
     4741 cgatgcgcgt aagctgccag ccgtggcagc cgtctcgcgg ccctcgcgac cggagctcga
     4801 cgacgtcaat gacttgttcg cagatttgac ggatccgagc gccgatcatt tcgcaccgag
     4861 ccaagccgac tcgaacccag ctgccgcaaa tcagaacatg cgctctcaag gctccgatca
     4921 cgctgccgtc acccgcctcg ggctggggcc tgacgatcca ggtcgcgaga gctcgctgcc
     4981 gcggccggga attcgcccgg ttctcagcat tcccgagggg ccgccgaaag catcgcccag
     5041 tatggccgac gtatttgcgg cggcgctgcg cgagagcccg aacgacagct tggcggtcga
     5101 gagcccagtt cccccgcccg tgtccgatcc ggttcgcacg cagacgttgc aggcggtgag
     5161 attgccggag cgagatgagt cctccgcgga attgattggc gcgtggtcag cgacgccgcc
     5221 gcctccagcg ccggccaccg aagccgcccc gaccacgctg ttgctcgaca acccgagtgg
     5281 tgcacggccc gccgccggag cccccgaagg cgcagcgggt cgcgcccgcg ccccggacaa
     5341 ccaagcggat gggcccgagc ccgctcccat tggcaagacc ttggtctcgg cgagcctgcc
     5401 cgaagccaag tcggattccg agcgcagctc cgcccgtcga ttggccgcca aggtcccgag
     5461 ctccgccccc ggctcgaagc cgccgagttc taaacccagc tcgaaatcga gcgcgaaagt
     5521 cgggacggag gcgaccgaca gcagcgcggg cagcggcgtt cgtacggtga tcttggcgtg
     5581 cgtcgcgttc ctgaccgcgt ttctcctcgt caactcggtg ttggtgcctt tgctggcccc
     5641 caaggaagcg cgcgaacccg cgaccacagt tgcggagcct gccaatcccg cgcccgactc
     5701 ggtggtggta accgagcggc tgcccatgcc cgcgggcgtg acgacgactc cggggagcgg
     5761 tctgctcgag gtggagttcg ccgccaccga agagctgcag gtgaacgggc aggtgcaggg
     5821 gcgcgggccc gtcagaatgg tgcctttgcc ggtcggaaaa cacaccgtgc gcgtgctgcg
     5881 aggggatgcc gggcgcgagg tccacatcga ggtgagcgag gggcaaagaa ctcgcgtgag
     5941 cttcgatcgc gcttcgaaat ccccgtgatg aggtgcgctt gggcgttggt tgttctcgcg
     6001 tgcgtgagct gtcgcgggtc gaaagaagag gcacctcgaa cgagctcgcc cgtggcatcc
     6061 ccgagcacgc cggcagcgga aattgccgaa gaggctgagc gctacgagct cacttcgcgt
     6121 ctggatgctt gcgacatcca gcacgatggc gcgctgattg acttcgggtc ccacggtacc
     6181 gagtctcgac gccattttgc cgtcggtccg ttcacggacg tcgctccgat cgatcgcgaa
     6241 ggcgcgagct tccagcaagt cttcagcaaa gccttgaatg tggaggtttg gctggaccag
     6301 ccgctgaata aaccgagcct gagtttacgc gcacacggag gaactgctcg cctcgtccac
     6361 gtgagtgtgg acgagcaacg cctcggcgca ttgcgcctgc cgaacggtga aacccgagtt
     6421 cttgcgacgg gcgcggccag cactccactc ccccgggggc gccaccgagt gaccttgcgt
     6481 ttctcgcccg caccgcgcgg cagcaagctg ccgctggtcg agctggattg gctgcgcttg
     6541 ggcgagttgc aggcggagcc tgccaagtac gccgctccga cttacgaaga catcgtcagt
     6601 gacgtcgccc tcgatcgcat tccgaagcat agcttggtgc tgcgcgcccc gagcagcgtg
     6661 cgctgcttct tgcgtccgtc cgccgactct cgactgaagg ttgccgtagg actatggggg
     6721 tccggcaaag gcagcgccga gatcaaattg ctgcgggatg cggagccgcc ggtacttttg
     6781 caatcccgca aagtgacggg tggagatggc gcgacctgga ccccggtcac cgtggatctc
     6841 ggtccctacg caggctccat cgttggcctc gagctctacg ccaacgaagc gacacgcggg
     6901 ggccgcattg ctttcggtga tccggtcctg gtgcggcaga cggagcgtcc gcccgtcagt
     6961 gcacgagcgc gcgtagcggt cgtcgtcgtg ttgtctgcgt tggagcgtcg tcgggtgccg
     7021 ccttggggcc caaccggaag cctggcgacc ctcgccgact tggcgcgcaa tgcgacggtg
     7081 ttcggcagtc accgtgttcc gagcagcgtt cccgcggcgg tactggcctc gctgctgagt
     7141 ggactctcgc cccgggcgca tgggctcgag gacccgaact ccaagttggc tccggagata
     7201 cacttgcttt cggaagccgt gaaggaagcc aacggacgca cggccatgtt cacgggcgcg
     7261 ccgacgtctt ttgcgacgtt tggtttcgac cagggctggg atgtcttcga cccaatctct
     7321 ccggtcaaag actatcctgc aagcgagcct ttgcagcgcg ccacccgctg gctcgagcag
     7381 gcgctggatg acgggctctc gggcccggtg ttggtggtgg tgcatgcgcg cggagcccac
     7441 ccgccgtggg acgtgacgcg ggaagaatcc cagcacctaa aaccccaaga ctacgccggc
     7501 atcgtcgacc cgcggcgcgg cggcatcctg atcggtgctt tgcgcagcaa gcccacccaa
     7561 cgcaacaaag tgcggcggat gagcgacgat gattgggcgc ggctgcgggc cctctcggac
     7621 gtcgcgctcg cgaagcaaga tgcggccatc gggcaaatgg tcggcatgct caagcgcaag
     7681 tcggcgtggg atgacaccgc cttcgtggtg acctccgacg tcgcgcccgg tgatccgcct
     7741 gacttcccct tcgatccgcg cgggccgctg accgaggatc gcttgctcgt cccgctttac
     7801 gtgcactttc ccggcaacaa cgtggggggc caggaagcgc gagcggcttc ggcgatgatg
     7861 gatctgaacg tcacgctgct tcacctgctc gggctgcgcg tgccgtcgag cctgccgtcc
     7921 gtcgatttgc tagcgcgagc gctcggtcgc gagccgatcg tcgtgcgcag tgagctcgcg
     7981 acgctgaatg acaagtattc ggcgcgcttg ggatcgtggc tgctgcgcgg tcagatcggc
     8041 agcgtcccga cgctttgcgc gctggacgtc gacccggcct gcgcgaccga taccttcgat
     8101 cgcgagctga tagcggcaag agcgctctgg caggcgacct ttcgggagct gaccgatgcg
     8161 cggaagctca cgcccaaaga cgaggtccgg cgcgtcacca ccatcgatga agacacggcc
     8221 gccgcgctca cggtctgggg cgatcaccag tgaatacgcc gtcggcgtga ctcgacccat
     8281 gccgggtggt ggctcttagc gaccgcgttc tccgccgaac atcaggacgt gcaatcgggg
     8341 tgagtagcgg accgcaagct ggcgggcgag ctccgcgacg tggtccccac ggccccggtg
     8401 ttcgggcacc gtgcgcgcct cgggcatcag gaatactcgc gactttggcc actcgagcgc
     8461 gctcaccacg gcctctacct cttctcggtc tcctgcgtct cgcaccacca gcttcaagta
     8521 ggcgcgctga gtcgcgagca gcacgcgcaa cacttccggc ttcattcgac gctcgagggg
     8581 ctcctccgag tgggcgagct tcggggagac gttccactga tcgactcgct gcagcaacga
     8641 ggcgttcgga atcagcgtgc cgttggtctc gacctcgatc tgccaatcgg acggcaactg
     8701 cgcgatcaac gagctgagtg ctcgctgttg catgatcggc tcgccgccag tgatgatcag
     8761 ccgccgttcg gagtgcgaca gaacacgagc caatacggat tcgaccggcg ccgtcgagac
     8821 ttctcgctcg tagtcgtact gctcgaaatc ccatgtatag cgggtgtcgc agaagccaca
     8881 ccgcaaattg cacaaggcta acctcaggaa caccgcgggc tcaccggcgc ttggtccttc
     8941 accttggacg ctcgagaaga tctcgctgag cttgagaatg tcttgggcag acatacgggg
     9001 aaatttctcg ttggggcttg aaactcgagc ggaggattgc tcccatgcgt cggccgtcat
     9061 cgaaccggcc gaatcacgag tcacatgccc tccgtatcac gtaccgaccg cagtagccgc
     9121 gccttgcgcc ccgtagacat gcaaatcggc tttcatcgcc ttgccgaagg ttccgtctta
     9181 tatcgagccg ggggcaccgt ggtgctttgc accgcgtcga tcgacaattc cgtgccggag
     9241 tttctcgccg gcaaggggca gggttggatc accgcggagt atcagatgca tccccgcgca
     9301 aaccctaaac gacgcgagaa tcgtgacggt cgcgagcggc cccttggtgg cagaacgcgc
     9361 gagattcaac gcctaattgg gcgcgccttg cggagcgcgg tggatctttc gcgcttggga
     9421 gagcgcacgc tcgccgtcga ctgcgacgtg ctggaagcgg acggcggcac gcgaaccgcc
     9481 tcgatcaccg gtggcttcgt cgcgatcgcc gccgccatgg caaagctcga ggcacggggg
     9541 gcgctacgca gtccggcgct acgggatcaa gttgccgcga tcagcgtggg gcacgtgggc
     9601 gacgaagtat tgctcgacct ctgctacacc gaagacagcg cggctcgcgt ggatctgaac
     9661 ctggttgcga cgcactccgg agcggtcgtg gagattcagg gaaccgcgga aggcgaggcc
     9721 attccgcgct cggagctgga ctcgatggtg gacctggcct tggtaggtat ccaggacctc
     9781 tgtcgtcttc aaaacgacgc attggccgca gcgggcatcg cgctcgatca gttgctcatc
     9841 ccgcgggcct gagcagctca tcctgccctt tcggccttgt attcttttcg aaggggcgtt
     9901 gggagggagc ttctagccgt cgcgcggctc cgag
//
LOCUS       DASTKU010001005        11225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1090417, whole genome shotgun sequence.
ACCESSION   DASTKU010001005 DASTKU010000000
VERSION     DASTKU010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11225
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090417"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFQ61_35640"
     CDS             complement(<1..896)
                     /locus_tag="VFQ61_35640"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET9959897.1"
                     /translation="MPRTRAQVTARIRVGISGWSYKPWRGVFYPPELRIKDQLKFASS
                     VFPTVEINGSFYSLQRPQTYRAWFDSTPESFIFSVKGGKFVTHLKRLTNSEQGLSNFF
                     ASGVLELEQKLGPILWQLPPTLGFDAARLKAFFEVLPRTFAEAGQLARGHAEWLKGRT
                     SLGPGGNSRIRYALEFRHPSFLCLEAIRLFREYRIAPCVADSAGLFPYTEDLAADLVY
                     VRLHGAEQLYTSGYSPQQLREWSARIRAWHAGRPGPDPHQVETSAKGDGAPRDVFVYF
                     DNDVKVRAPFDALNLERILRGA"
     gene            1543..3183
                     /locus_tag="VFQ61_35645"
     CDS             1543..3183
                     /locus_tag="VFQ61_35645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9959898.1"
                     /translation="MNDSTAESQLRGCLTSEYELVATTIDELDAECARGEPALVLIGC
                     VDEREPASSRSFSSRAVSSGSLSSSPPSLAPRSAAEVVVALRARRSLASTPVLLLTRP
                     AGEAHAVSLLDRGAHDYVVWPCLDQVLRARTRNLISLHEAQRDAALSTAREQMTRREF
                     EQLRGSAESGTRAKEEFLAMLGHELRNPLSPILTALQLMRLQGPEVSDRARTVIERQV
                     NHLTRLVDDLLDVARIARGKVELKQERLEISVAVAKAIELASPLLEQRKHALSVSVPN
                     RGLCVNGDVARLSQVISNLLTNAAKYTSPGGQITISAEAVADQVEIAVSDTGMGIAPE
                     VLPHIFEAFVQERQAIDRSQGGLGIGLTIVRNIVEQHGGSVSAGSQGPGRGSRFLVRL
                     PRLTAPLSEAAEAPPKSEPAKMALCGHALRILVVDDNEDGAEMLALALSRRGYETRVA
                     HDAPTALRAAAEFAPDVAFLDIGLPVMDGYELAAHLRLLPGLTGLRLIAVTGYGQESD
                     RRKTREAGFQQHLVKPVNIEIVEAALLGRGECIQEQSF"
     gene            complement(3209..4687)
                     /locus_tag="VFQ61_35650"
     CDS             complement(3209..4687)
                     /locus_tag="VFQ61_35650"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9959899.1"
                     /translation="MGGVIPGSVLAGRYRLERQLGEGGMGSVWLAEHLALRSRVAIKI
                     MDPAIASNHEGAERFRREAQAAASLRSTHVVQVLDFGLDELGPFLVMEVLQGQSLSHI
                     LQRTPRLDPRRVGWIIAQVARAMTRAHAANIVHRDLKPDNIFLVREDDQEIVKVLDFG
                     IAKTIDPRFGGLQTQSGVTMGTPHYMSPEQAAGKRTVDYRTDLWAMGMIACECLVGRR
                     VIEGDGWAPLLVAICVQPLPVPSTLGPVPPGFDAWFARATERDPERRFASAKELADTL
                     VTLVGGPPGLDSEPPRQISGRGATVRTDSPLEARGPVTAPVLAATVIDAPAGRDRLVN
                     AGAPPSKLRRRSWLWFAIPGAGIALTSVSLGYWLLQGNSIAPELNPTPLPSASAEALQ
                     PAAGPNPVEPQVVPDTPDTQSSHSGDPSRGTRSTPRRNDIAANGTPANGPRPNKEGSG
                     KGPPTLMCDTDPFTGAVRPARQPSPGSFPCAQNPFTGVYVRR"
     gene            4828..5859
                     /locus_tag="VFQ61_35655"
     CDS             4828..5859
                     /locus_tag="VFQ61_35655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959900.1"
                     /translation="MTGVLAAHIVEFMHRGAPYLLIVASFFGAAVIPACARYKPARTY
                     EVRSQADPAGTYQVVQGVLKDYGYTIVAQDAAQHSVSVRSHAFETNNKRQSWIAIEVQ
                     PDGLVKLAGSGYLVRPDGSLHARLRNELDVLESKIQERLVALGPTTANAGSSAGQASS
                     PGNATDASGTDMNVPGYDKGAGTGAPAASSNELPKAWSEPAYDPKVWGPGQFTCVPVH
                     LSKSSNAFRLKLSNGEFADVLVSVAYAPELCRSPSECPLKEGCPAVGLGDNSKVSFLA
                     QRLDRGEIQGEAILFDGPTPQVKLDLRNHGSIVQAQAASKTPAPAAKAPAPKTPAPKT
                     PAPKASGPK"
     gene            complement(6153..6359)
                     /locus_tag="VFQ61_35660"
     CDS             complement(6153..6359)
                     /locus_tag="VFQ61_35660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959901.1"
                     /translation="MTKFRNPLSTLAAVNPTAPGMAPTTESSSVLQASVAADKKSHLA
                     RAFTVHTRVSPKKVTSFARRMRHK"
     gene            complement(7061..8011)
                     /locus_tag="VFQ61_35665"
     CDS             complement(7061..8011)
                     /locus_tag="VFQ61_35665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959902.1"
                     /translation="MLHGKRSRPSLWATLSAAAMVFALTRPAAAAVTLCVETHTEAED
                     QVGFRKLVMSELARHPSHQVVPADCQSRLRVDSFRTSGKRYLTVQLDGEVPVRTSLAE
                     NTDVAPILTDSMAQVLRNDPMYLAEDPNRWSAVERASRSVLIRGMNTYRVELFETAAR
                     TDRNVAFAPGVALGFTRGADHWQVSARLHLAGDASRLERHDRALRLATGMDLGLTWEA
                     SRRAPTSAYVSAGGGLSVLRFAGYLDEQNENSLTDVYRWLPTVHLRGGIRLLRVFDFD
                     ADAFLQATLPLLPTSQVDDELWGERGVWTPLFLTGLGVGF"
     gene            complement(8021..9400)
                     /locus_tag="VFQ61_35670"
     CDS             complement(8021..9400)
                     /locus_tag="VFQ61_35670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959903.1"
                     /translation="MERTITSLVALMFGVSLAPQALAQKKPEVAVCVSVTLHTPPKPV
                     KEEPPSVPETPAEKPSETSSPSSSSAATPEGAAPKPTSTPAPAQPAASAPPPSLFGPV
                     ADEPAAPFTKAATASARPRNMDDSDVSADAAADLASAVDPENRRDPRVSSGAHLPLGQ
                     TPTVYLKRLMDHFITHEPGFVANGSCTEHVEVELYPLKVGWTAFARYSGTGREERVDQ
                     LLPTELSQFAERSVLALLHDVPISSTVSRDNVLWADSLKSTQRIRGNGHFAMGVGTRL
                     RAGEFATLDADREGTSDEVSRKYRLFTPIALSLGYRGQFEEYGFEATGELDVGTSVTG
                     VRHNPSGGNIDYGGSFGVALHVLRYTQPRALTSPYFGAGATFALHWFQATRAQPERTT
                     ESRSRLYGGGLDVDVVGGYEFMRASAISFYLQGELTLPAYIVRTENNDGRINTWFPGA
                     ALRLGANFF"
     gene            9699..10493
                     /locus_tag="VFQ61_35675"
     CDS             9699..10493
                     /locus_tag="VFQ61_35675"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET9959904.1"
                     /translation="MIDIGVNLTHKQFHTDISQVIERALAAHVNHLIITGLNVPVSRA
                     ALTLARSRPSALCCTAGIHPHNASSFGAASSGELRELGAAREVVAIGECGLDYERDFS
                     PRPAQRAAFEAQLALAADLQRPVFLHQRGAHADFLRILSEYSPRLIGGVVHCFTGDVP
                     ELESYLALGLCIGITGFICDERRVTHLQALVRRIPSDRLMIETDAPFLLPRDLKHANG
                     RRNEPAYLPHIAAAVARYSGRTEQEVRRTTTEVAFRLFGLARVFSA"
     gene            complement(10483..>11225)
                     /locus_tag="VFQ61_35680"
     CDS             complement(10483..>11225)
                     /locus_tag="VFQ61_35680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959905.1"
                     /translation="RVRAAMRLHPFGVLLLFAGISGMGARTEVARANEQASRADRAPE
                     HRNDRVLEQLAQISRNLRESKYNHVTRVNEREGRYEFDCSGFVTWVLRRVAPGAYAAV
                     MSRSKTGRPVARDYYWEFARAPTARSPRGVLRIERVAEAQAGDIVAWLKPALVQSPNT
                     GHVGFLLERPRPVPQLPGGYLVRIADASSYQHQDDDRSDSGRTGFGSGTILLVANLET
                     GAAQAYGWFGLQSYYVLETPIAIGRVTR"
BASE COUNT         2037 a         3436 c         3706 g         2046 t
ORIGIN      
        1 tcggcacctc gcaggatgcg ctccagattg agggcatcga aaggggcacg tactttgacg
       61 tcgttgtcga agtagacgaa cacatcgcgc ggcgcgccat ctcccttcgc cgaggtctcg
      121 acttgatgag gatccgggcc aggccgaccc gcgtgccatg cgcgaatacg cgcagaccat
      181 tctctgagct gctgaggact gtagccactc gtgtacaact gctccgctcc gtgcagccgc
      241 acgtagacga ggtcagctgc caaatcttcg gtatacggaa agaggcctgc cgagtcggcg
      301 acgcagggcg caattcgata ctcgcggaac agccggatgg cttcgaggca gaggaagctc
      361 ggatgtctga actcgagcgc atatcggatt cgcgagttgc cgccgggccc gagtgaggtg
      421 cgtcctttca accactcggc atgtccacgt gcgagctggc cggcttcggc gaacgtgcgc
      481 ggcagaactt cgaagaaggc cttcaatcgt gccgcatcga agccgagggt gggcggcaac
      541 tgccagagaa ttggcccgag cttctgctcg agctcgagaa cgccggaagc gaaaaaattg
      601 gaaaggcctt gttctgagtt ggtcagtcgc ttgaggtgtg tgacgaactt gccgcctttg
      661 accgagaaaa tgaacgactc gggtgttgaa tcgaaccaag ctcgataagt ttgtggacgc
      721 tgcagcgaat agaaagagcc attgatttca acggtcggaa agaccgagct cgcgaacttc
      781 agctggtcct tgatacgcag ctcgggagga tagaatacgc cgcgccaggg cttgtagctc
      841 caaccggata tcccaactcg tattcgggct gtgacttgcg cacgagtgcg aggcatctat
      901 cgataaacga ctcggagttt tagctgccgt ggttgagtgg tagtggcagc gcgtgcaatc
      961 cacgttccag gtcccaggct tccaaaccta gggcttcgcg gctccggcgt ggccgttttg
     1021 taccaaactc accgaactgc ctgcgacacg ggtttgagat tcgattctta cactaaaggg
     1081 ttatcgcgtg gccaagaccc gctgtcgagg tgtgtgggaa tcatgctcga agccaaccgc
     1141 tcccgcatcg aatccgtggc tcgtttcgaa aatatgcggt cgagttcggt tgacagctcg
     1201 gcgtgtcgat tgaaaactga cgagtccacc atgttccgtg acgagctgcc tgatgggctg
     1261 tccaaaaccc attcgcctgg ggattcacct gcgggttatg cggggcgagt gctcggggaa
     1321 gggtcggatc ggactgcggg acccatggct gcaggggcga tcgaggctcg tggagcaccc
     1381 atttccgatc gaacgagaca acgcgcacca agagcgaatc gccaatcctc acccgagatt
     1441 ggggcggtgc acctacgtag gttggagggc acacgggcgc gttcagaaga gagggcaacg
     1501 gcacatttgc cagcgttcgg acgcgcgaag atctggctgc ttttgaacga ttcaacggcg
     1561 gagtcccagc tgcgggggtg tttgacgtcg gagtacgagc tggtcgcgac tacgatcgac
     1621 gagctggacg ccgaatgtgc gcgtggagag ccggccttgg tgctgattgg ctgcgtcgat
     1681 gaacgagagc ctgcctcgtc gcgctccttc tcatcccgag cggtctcatc cgggtcgctg
     1741 tcgtcgagcc ctccgtcgct cgcaccacgg agcgcggcgg aggtggtggt ggctctgcgc
     1801 gcgcgacgtt cactggcgtc tacgccagta ctgttgctca ctaggccagc cggcgaagca
     1861 catgccgtga gtttgctcga tcggggtgct cacgattacg tcgtctggcc ctgtctggat
     1921 caggttttgc gggcgcggac gcgcaatttg atatcgctcc acgaggcgca gcgtgacgcg
     1981 gcactcagca ccgcgcgaga gcagatgaca cgtcgggagt tcgagcagct gcgcggctcg
     2041 gccgagagcg gaacccgcgc caaggaagaa tttctggcga tgctcgggca cgagctccgc
     2101 aatccactgt ccccgatcct gaccgcctta cagctgatgc ggctccaagg tcccgaagtg
     2161 tcggatcgag ctcgcacggt gatcgagcgt caagtcaacc accttacccg tttggtggac
     2221 gatctgctcg acgtggcccg catcgcgcgt ggcaaggtgg agctcaagca ggagagactc
     2281 gagatctcgg tggcggtggc gaaggcgatt gagctagcga gccccctcct ggaacaacgc
     2341 aagcacgcgc tctccgtatc cgtaccgaac cgcgggcttt gcgtgaacgg ggatgtcgct
     2401 cgactcagcc aagtcatctc gaacttgctc acgaacgccg ccaagtacac ctcgccgggt
     2461 gggcaaatca cgatctcggc ggaggccgtg gcggatcagg tggagatcgc cgtgagcgat
     2521 acgggcatgg gcatcgcgcc ggaggtgttg ccgcacattt tcgaagcgtt cgtgcaggaa
     2581 cgtcaggcca tcgatcggtc gcagggcggg cttgggatcg ggcttacgat tgtgcggaac
     2641 atcgtcgaac agcacggtgg cagcgtcagc gcaggaagtc aggggcctgg gcgcggcagt
     2701 cgcttcctcg tgcgcttgcc gaggctgaca gcgccattat ccgaagcggc ggaggcgccg
     2761 cctaaaagcg agcccgccaa gatggcgtta tgcgggcacg cgcttcgtat cttggtggtc
     2821 gacgacaacg aagacggcgc cgagatgttg gccttggcgc tctcgcgtcg agggtacgag
     2881 acgcgcgtcg ctcacgatgc tccgacggct ttgcgtgccg ccgccgagtt cgcgcccgac
     2941 gtcgctttcc tcgacatcgg actgcccgtc atggacggtt acgagctggc ggcgcatttg
     3001 aggctcttgc cgggcctaac gggcctgcgt ctcattgccg tgactgggta cggccaggag
     3061 tcagaccggc gcaaaactcg cgaagccgga tttcagcaac acctcgtcaa gccggtcaac
     3121 atcgagatcg tcgaagcggc gctgctgggc cgcggcgaat gcatccaaga gcagagtttt
     3181 tgacagcgcc gggagaagct ctgcggagct agcgccgaac ataaacaccg gtgaacgggt
     3241 tctgggcgca cggaaacgag cccgggctcg gctgacgcgc ggggcgcacc gcacccgtga
     3301 atgggtcggt gtcgcacatc aaggtgggtg gacccttacc tgagccctcc ttgttcgggc
     3361 gaggaccatt cgcaggcgta ccattcgccg caatatcatt gcgccgcggt gtcgaccgcg
     3421 tgccgcgcga gggatcgccg gaatggctgc tctgcgtgtc tggcgtatcg ggaacaacct
     3481 gcggttcgac gggattcggc cccgccgcgg gttggagcgc ttctgccgag gcgctgggca
     3541 aaggggtagg attgagctcg ggagcaatcg agttcccttg gagcaaccag tagcccaagg
     3601 agacgctcgt caacgcaatg ccggcgcccg ggatcgcgaa ccacaaccac gagcgtcgtc
     3661 ggagcttgct cggaggcgcc ccggcgttga cgagacgatc ccgcccagcg ggagcgtcga
     3721 tcaccgtagc ggccagtacg ggggccgtca ccggtccgcg tgcttcgagc ggagagtcgg
     3781 tgcgcacggt cgcgccgcgc ccgctgattt ggcggggcgg ttcgctatcc aaaccaggcg
     3841 gcccacccac tagcgtcacc agcgtatcgg cgagctcctt ggcggaagcg aagcgccgct
     3901 ctggatctcg ctcggtggca cgcgcaaacc aagcgtcgaa acccggaggc accgggccta
     3961 gtgtcgaagg cacgggcagg ggctgcacgc agatcgcgac gagcaagggg gcccagccgt
     4021 cgccctcgat cacgcgccga cccaccaagc actcgcacgc gatcatcccc atcgcccaca
     4081 agtccgtgcg atagtcgacg gtgcgcttgc ccgctgcctg ctcgggactc atgtagtgcg
     4141 gcgttcccat ggtcacgccg ctctgggttt gcagtccgcc gaagcgcgga tcgatggtct
     4201 tggcgatgcc gaaatccagc accttgacga tctcctgatc gtcctcgcgt accaggaaaa
     4261 tgttgtccgg cttgaggtcg cgatgcacga tgttcgcggc gtgcgcgcgt gtcatggccc
     4321 gcgcgacctg cgcgatgatc cagccgacac ggcgcggatc gagccgcggc gttcgctgca
     4381 aaatgtggga caggctttga ccctgcagca cctccatgac caagaatgga cctagctcgt
     4441 ccaacccgaa atcgagcacc tgcacgacgt gcgtgctgcg cagtgatgcc gcggcctgcg
     4501 cctcccgccg gaagcgctcc gcgccctcgt gattggacgc aatggccgga tccatgatct
     4561 tgattgcgac ccgcgaacgc agggccagat gctccgcaag ccataccgag cccatgccgc
     4621 cttcaccgag ttggcgctcc aagcggtatc gccctgccaa cacgctgccg ggaatcacgc
     4681 cgcccatggc cgctttcctg ctgggatgag gagcgtgaac cgagggtcac acgggtctgg
     4741 gtcgcaccgt tctcgatccg agggttctcg cgggactggt tctccgcccg cgccggctct
     4801 gcgccaggcg agcgttggag cgtagcgttg acgggtgtgc tcgctgctca tatcgtggag
     4861 ttcatgcacc ggggtgcacc ctacctgctg atcgtggctt cgttctttgg cgcggcggtg
     4921 attcctgcct gtgctcgcta caaaccagcg cgcacttacg aggtgcgtag ccaagcggat
     4981 cccgcgggca cgtatcaggt ggttcaaggc gttctcaaag actatgggta caccatcgtc
     5041 gcgcaggacg ctgctcagca cagcgtcagt gtgcgcagcc acgccttcga aaccaacaat
     5101 aaacgtcaaa gctggatcgc gatcgaggtg caaccggacg gtctcgtcaa gctggcgggt
     5161 agcggttacc tcgttcgccc tgacggttcg ctgcatgcgc gtctgcgaaa tgagctcgac
     5221 gttctggaat ccaagatcca ggaacgtctg gtcgctcttg gaccgacgac cgccaacgcg
     5281 ggaagcagcg cggggcaagc gtcgagccca ggaaatgcaa ctgacgcctc tggaaccgac
     5341 atgaatgttc caggttacga caagggtgcc ggcaccggcg cccctgctgc gtccagcaac
     5401 gaattaccta aggcctggag cgaacccgca tacgacccca aagtctgggg gccaggtcag
     5461 ttcacctgcg ttcccgtcca cctctcgaag tcatccaacg ccttccggct gaagctctcg
     5521 aacggcgagt tcgcggatgt gctcgtgtcc gtggcgtacg cgccggagct atgtcggtcg
     5581 ccctccgagt gcccgctgaa agaaggctgc cccgcggtgg gcctgggtga caacagcaag
     5641 gtttccttct tggcgcaacg cttggatcgc ggcgagattc aaggtgaagc gatcttgttc
     5701 gacggcccaa ctccgcaagt caaactcgac ctgcgtaacc acggctcgat cgtgcaagct
     5761 caagccgcgt cgaagacccc ggcaccagcc gcgaaagccc cggcaccgaa gaccccggca
     5821 ccgaagaccc cggcaccgaa agcgtccggc ccgaagtgag ttgctgacgc catttctcga
     5881 gtggatggcg ttactgggtg cggcgggcgc ggtgcggcgg gcgcgtggag cgctttggct
     5941 gcgctggtga gcaccggcaa cgggcggcgc gcacgtgatt tcgagccgac ccaatcactg
     6001 gaaccaataa agtgagcgct cgcaaagcct cggcaagccg ggctttgcga gcaaactcga
     6061 cgaacgaagg tcactagccc gagcgggacc ctagtgccgt gcacgtgagc caacgacctc
     6121 gggtcccggc gctagggctt cccgcgtgtg aatcacttgt ggcgcatgcg gcgcgcgaag
     6181 ctcgtgacct tcttggggga aacgcgcgtg tggactgtga acgctcgagc gaggtgggac
     6241 ttcttgtccg ctgccacgga agcctggagc acggatgacg actcggtggt cggcgccatc
     6301 cccggggcgg tcgggttcac ggcagcgagg gtagacagag gattacgaaa cttggtcatg
     6361 atgctctcga ctgttctgag ccgatgcgcc cgcgagtgat gagggcgatc tcgcgataca
     6421 cgtgtgcccg ccggctcgac cggtacacac gacagttatc gagtcgatga tcggatatcg
     6481 aacacccgat catcctcgag tcggacgtga gcagtggggc acgcgccacg cgacgcgcac
     6541 ccgacgagat tccgaatcag tcagcgtcca gcacgcacct gacgagcgat gaccagatat
     6601 ggtcagggct ccgctcgcga ggacaacccg cgtcgccgag tagttacggc tctcgaagct
     6661 tccagtgcgg aaatgccgaa gccccgcaac ggccccgcga ggtttcgagt gcattctgaa
     6721 cgctagcttt tcggagcgcc ggcattccgc gccatttcag ggcgaaaccc gctttcgggt
     6781 tagccttgtt gatggcgagt aggcctgcgc tgatcgcacg cagctcgacc gagagggctt
     6841 cgagctgtgg caaaccgcag ccaaacacct gaccgatacg cgagttgccg atgccactgc
     6901 tcaggtggct aggcgtaacg cgattggctc agcgttggac gggtccgaag ggcgtcagag
     6961 aaagttgtgc atggaagtcg gttggagccg acagggcgta agatggctga ccgctcacag
     7021 actgtcaatc cacaaatcca gtgcacggtt cgagacgaga tcagaagcct acaccgagtc
     7081 cagttaggaa taggggtgtc caaacgcccc tctcgcccca gagttcgtcg tcgacttggc
     7141 tggtcgggag cagcggcaat gtggcttgca ggaaggcgtc ggcatcgaag tcgaagacac
     7201 gcagcaatcg gatcccgccg cgcaagtgga ccgtgggcag ccagcggtat acgtcagtga
     7261 ggctgttttc gttttgttcg tctaggtaac ctgcgaagcg cagcacactg agcccccctc
     7321 cggcgctcac gtaggcgcta gtcggcgcgc ggcgcgaggc ctcccaggtg agtccgaggt
     7381 ccatgccggt cgcaaggcgc aaggcccgat cgtgccgctc caggcggctc gcgtcgccgg
     7441 ctaagtggag ccgagccgag acttgccagt ggtctgcccc gcgcgtgaag ccgagcgcca
     7501 ccccgggcgc aaaggccacg ttgcgatcgg tacgcgcggc ggtctcgaac agctccaccc
     7561 gatacgtgtt catgccacgt atcagcacgc tgcgactggc gcgttcgacc gcggaccaac
     7621 ggttgggatc ctcggcgagg tacatcggat cgttacgcag cacttgcgcc atcgagtcgg
     7681 tcaggatggg cgcgacgtcc gtgttttcgg cgaggctggt gcgcacgggg acctcaccgt
     7741 cgagctgcac ggtgagatag cgcttgccgc tcgtcctgaa ggaatctacg cgcaaacgcg
     7801 actggcagtc tgcaggcacg acttggtggg aagggtgtct cgcgagctct gacatgacga
     7861 gcttacgaaa ccctacttga tcctccgctt cggtgtgcgt ttctacgcag agcgtgaccg
     7921 cagccgcggc cgggcgcgtc agcgcaaaga ccatggcggc cgccgatagt gtcgcccaaa
     7981 gcgacggtcg actgcgtttg ccgtgcagca cgcatcacct ctagaagaag ttggcgccga
     8041 ggcgcagcgc ggcgccgggg aaccaggtgt tgatgcggcc gtcgttgttt tcggtgcgca
     8101 cgatataggc ggggagcgtc aactcaccct gcaaatagaa cgagatcgcg ctggcgcgca
     8161 tgaactcgta gccgcccacg acgtcgacat ccaatcctcc tccgtaaagt ctcgagcggg
     8221 actcggtggt gcgctcgggc tgcgcccgtg tcgcctggaa ccaatgcagt gcgaaggtag
     8281 cgcccgcacc gaagtacgga gaggtgagcg cgcgcggctg cgtatagcgg agcacgtgca
     8341 gcgcgacccc gaagctgccg ccatagtcga tgttgccgcc gctcgggttg tggcgcacac
     8401 ccgtgacgct ggtgccgacg tccaactcgc cggtggcttc gaagccgtac tcctcgaatt
     8461 ggccgcggta gccgagtgag agcgcgatgg gtgtgaagag gcgatacttg cggctgactt
     8521 cgtccgacgt gccctcgcga tcggcgtcga gggtcgcgaa ctcaccggcg cgcaagcgtg
     8581 tgccgacgcc catggcgaag tggccgttgc cgcggatgcg ttgggtgctc ttgagtgagt
     8641 ctgcccacaa cacgttatcc ctcgagacgg tggagctgat cggcacgtcg tgtagcagcg
     8701 cgagcacgct gcgttcggcg aattgggaca gctcggtggg tagcagctgg tccactcgct
     8761 cttcacgccc ggtccccgaa tagcgtgcga acgcggtcca acccaccttc aacggataca
     8821 gctcgacttc cacgtgttcg gtgcagctcc cattcgccac gaagcccggt tcgtgcgtga
     8881 tgaagtgatc catcaaacgc ttcaggtaga cggttggcgt ctgaccgagg ggcaagtgag
     8941 cgccgctcga aacccgtgga tcgcggcggt tctcggggtc gaccgcggag gccaaatccg
     9001 cggcggcatc cgcgctgacg tccgagtcat ccatgtttct ggggcgggcg ctggcggtcg
     9061 cggcttttgt aaatggtgcg gcgggctcgt cggcaacggg accgaacaag cttggcggtg
     9121 gagcgctcgc cgcgggttgc gcgggcgcag gcgtgctcgt gggtttcggc gccgcaccct
     9181 ccggggtggc tgcggacgac gaggagggcg acgacgtttc ggacggcttt tcggcgggcg
     9241 tttcgggaac ggagggcggc tcttccttca ccggtttcgg cggcgtatgc agggttaccg
     9301 agacgcaaac cgcgacttcg ggcttcttct gcgccaacgc ctgcggggcg agagatactc
     9361 cgaacatcaa ggcgaccaaa ctcgttatcg tacgctccat cccatactcc tcgcgatgct
     9421 ccgctctggg ggcacagcgc ggaacagcaa cgcgtgtgcc acgctcgcgg gaggcgcgct
     9481 tctgcatgaa atataggcaa ttggcgtgag ttgggctcga gctgtggctc gcgcgtgtct
     9541 cgttcgagct tcggagtgaa gctcgttcac acgtcagcgg ttctcggtct ccggtgccct
     9601 gctcgcatca ctctacggca gctggattct cagcagagcg gcttgctctg gtcgagaggg
     9661 cgcgctcgtc gcatactcct cgcgctttgc aagaaccctt gattgatatt ggtgtgaatc
     9721 tcactcacaa gcagtttcac acggacattt cccaagttat cgagcgtgcg ctggccgcac
     9781 atgtgaatca cctcatcatc accggcctga acgtgcccgt cagccgggct gctctgacgc
     9841 tggcgcggtc acggccgtct gctctgtgtt gtacggcggg gattcatccc cacaacgcga
     9901 gcagcttcgg tgctgcgagc agcggagaac tacgggagct gggcgcggct cgtgaggtgg
     9961 tggcgatcgg cgagtgcggg ctcgactacg agcgggactt ctctccgcga cccgcccagc
    10021 gtgcagcctt cgaggcgcag ctcgcgttgg ccgctgattt gcaaaggccc gtcttcttgc
    10081 accagcgcgg agcgcacgca gattttctcc gaattctgag cgagtactcg ccgcgcctga
    10141 tcggcggagt ggtccactgc ttcacgggag acgtgccgga gcttgagtcg tacttggctc
    10201 tggggctgtg tatcggcatc accgggttca tttgcgacga gcgtcgtgta acccacctgc
    10261 aggcgttggt tcggcgcatc ccgagcgatc gcctgatgat cgaaacggat gctccgttcc
    10321 tgctgccgcg cgacttgaag cacgcgaacg ggcggcgcaa tgagccggcg tacttgccgc
    10381 acattgcggc ggcggtcgcg cgctattcag ggcgtacaga gcaggaggta cggcgcacca
    10441 ccacggaggt ggcgttccgg ctcttcgggc tggctcgcgt gttcagcgcg tgacccgacc
    10501 gatggcgatc ggagtctcga gcacgtaata actctgcaag ccgaaccatc cgtaggcttg
    10561 tgccgcgccc gtttctaagt tcgcgaccag taagatggtg ccgctgccga agccggtgcg
    10621 ccctgaatcg ctgcggtcgt catcttggtg ctgataagaa gaagcgtccg cgatgcgcac
    10681 caagtaaccg cccggtagct ggggtacggg tcttggccgt tcgagcaaga atcctacgtg
    10741 gcccgtgttg ggcgattgca cgagcgctgg cttgagccag gccacgatgt ctccggcttg
    10801 cgcttcggct acgcgctcga tccgtagaac tccacgcgga gagcgcgccg tcggcgctcg
    10861 tgcgaactcc caatagtagt ctctcgcgac gggacgaccc gtcttcgaac gggacatcac
    10921 ggcggcgtat gcgccaggcg ccactcttct gagcacccac gttacgaaac ccgagcagtc
    10981 gaactcgtag cggccttctc tctcgttgac ccgcgtcacg tggttgtatt tggactcgcg
    11041 gaggttgcgc gagatctgcg ccagctgctc gagcacgcgg tcgttacgat gttccggggc
    11101 acggtcggct cgggaagcct gctcgttggc tcgagccacc tcggtccgag ctcccatgcc
    11161 gctgattccc gcgaacaaga gtaggacccc gaacggatgc aggcgcatgg cagctctgac
    11221 acggg
//
LOCUS       DASTKU010001006         4991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1985783, whole genome shotgun sequence.
ACCESSION   DASTKU010001006 DASTKU010000000
VERSION     DASTKU010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985783"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2132)
                     /locus_tag="VFQ61_35685"
     CDS             complement(<1..2132)
                     /locus_tag="VFQ61_35685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959906.1"
                     /translation="MIRNRVFHSLLVLSNLVLACGGGGDPTAPGAGGDNPGSTSGGKP
                     AASGGQSADASGGRSSGGRSSGGGATSSGGASSGGGAGPGGSSSVEMGGFAGTLGGAG
                     SGAQGNSGGQHTSGGNTEGGAPAGGATSGGAASGGTAAGGTSAGGMGAGGTAMGGFTG
                     GTAGAPSQCAAGESCEHPNPCVDQAFTECSSGMERCEARSTLADGTSCGSGQVCNGGA
                     CVPCASGMACSVPGTECKAGQLSCDTGTAVCKPVGNVQNGIACSQGVCANGACVESCV
                     PDQPCGDSSSCHVSRTSCASGQEQCVKTSESLPDGTSCGVSSFCYSGVCEPCQAGGSC
                     TPAQACKTGTYACTPQPYACAVTGNMPDGTACGSNQVCDEGACVACVAQASCVPSDPC
                     KLGQLDCSAGPVCVATASNSPDGTACGADRVCTAGTCHSAVRVLELSGSAPASKRINE
                     ATELVTFTLEDVESARIAGAVVAITAPEGAYAPLSVTTDAQGIGSFRARLGRIPGPQV
                     FTLKTPGAEPLQVTFTATTPNTGEIFTLTNIDRAPSDSALPAPGPVAHTFYLTEIAVA
                     QDGAAYVSENCRVLKLTPAGELRVLAGGVSCGFQGDLGPAASARLSGIQGLALDEAHQ
                     TLYVADTGNQRIRSVNLANGTIVTFAGGGSAIGPGYGDGGPRRSAVFDRPDRIMLGPD
                     GALYVSDSGHNRLRRIDAVSGIVTAWGE"
     gene            complement(2281..4203)
                     /locus_tag="VFQ61_35690"
     CDS             complement(2281..4203)
                     /locus_tag="VFQ61_35690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959907.1"
                     /translation="MRSIKVVSGALTVFSMAFAAAGCNGSSDPAPGSSSGGAGTTQET
                     SRGGARQETSASSGGSSSSGGSSEQTGGWATEDHASAWIGPSGGELKLGKMALQIAPG
                     ALQEEVEISVDRYELDLEKYTALSAVYDFSPAGLSFDSPATLSFELDRAADADAKLYR
                     GESADAELLPVTSARIEGTSVVAQIGGFSHYVVAKLMPGPGPGIGNSFEVDGAIYSAY
                     PVTSGAIEGFSYTVLKALTVTDGEAGGHVWYGSNYCLTFRANGTGVIDMKGGANTRLS
                     SFPWLTVDWKPGTATALVQGPSRFSPAGSSMLDPDYVRAVELHPFEWGVRINADGTPY
                     ESFLGAYEYYLKFDESSPISSSTWTWNVDGGPTSLTATPPEDSHFCYFHEQAFTSGAS
                     PSRCIESQIIGFGNNATQKQCYSKGALEGEYSLVDNDTGVTLEAGEFSGNKPSGYWRF
                     ESKAGIPIAKGEFDEFGMQRGKWEIYDFEGNLESIPTFRGGIEPSRRGYYRVLEGSYQ
                     KFTLPWGGSASYLSEEGSYLDGHKNLWWTGFGPLQELSYMEEYDASTQLPSGWNTCYK
                     DEAGEPECVDHTYSWMKQRISYDWGRCVRGVNTVTVNTFNQDNELVEQTCYELSGTPA
                     EPQRGAQKKCGSACGA"
     gene            complement(4537..>4991)
                     /locus_tag="VFQ61_35695"
     CDS             complement(4537..>4991)
                     /locus_tag="VFQ61_35695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959908.1"
                     /translation="YAQAPAAPSNSAPFGCSKEMVGQQLASFCHIQTRGYHPDTGEYG
                     QTGIVHLWIYWGASDTGGWMCITDTWTNGRGVKVKIMPHIRGRPADGSAANYVVFGPY
                     KDTAGDCTWQPSFRTASGTDIDSVRVEWGDSWNKCDYCNVESQVVTRP"
BASE COUNT          927 a         1680 c         1441 g          943 t
ORIGIN      
        1 tgctcgcccc aggcggtcac gattccgctc acggcatcga tgcggcgcaa acggttgtgt
       61 cccgaatcag aaacgtacaa agcaccgtcg gggcccagca tgatgcgatc cggtcgatcg
      121 aacactgcag accggcgcgg ccccccatcc ccgtagcccg gtccaatcgc gcttccccca
      181 cccgcaaagg tgacgatagt tccgttcgcg aggttcaccg agcgtatgcg ttgattcccc
      241 gtatccgcga catacagggt ctgatgcgcc tcatcgagcg caagaccttg gatcccggag
      301 agtcgggccg atgcggcggg cccgaggtcc ccctgaaaac cgcaagaaac gccacccgcc
      361 agcacccgca attccccggc cggggtcagc ttcagtaccc gacagttctc gctcacgtac
      421 gcggctccat cttgcgcaac cgcgatctcg gtcaggtaga aagtatgcgc gaccggcccc
      481 ggcgcgggca aagcggaatc gctaggtgca cggtcgatgt tggttagggt aaagatctca
      541 ccggtgttgg gggtggtcgc ggtgaacgtg acctggagcg gctcggcccc gggggtcttg
      601 agtgtgaaga cctgcgggcc cgggattctg ccgagccgcg cgcggaagct gccgatcccc
      661 tgtgcatcgg tcgtcacgga gagcggagca taggcgccct cgggggcggt gattgcgacc
      721 acggctccgg cgatacgagc gctctcgacg tcttcgagcg tgaacgtcac gagctcggtg
      781 gcttcgttga tgcgcttgct cgcgggcgcg ctccccgata gctcgagcac gcgaactgca
      841 ctatggcacg tccctgcggt gcagactcgg tccgcgccgc aggcggtacc atctggtgag
      901 ttcgatgcgg tcgcgacaca caccggaccc gccgagcaat cgagttgccc gagcttacag
      961 ggatcggagg ggacacaaga agcttgcgcg acgcacgcca cgcaggcccc ctcgtcgcaa
     1021 acttgattgg atccacaggc ggtgccgtct ggcatgttgc cggtcaccgc gcaggcatac
     1081 ggttgaggag tgcaggcgta cgtgcccgtc ttgcaggctt gcgcgggggt gcagctgccg
     1141 ccggcttggc acggctcaca cacgcccgag taacagaatg agctcactcc acacgacgtt
     1201 ccgtccggga ggctctcgct cgtcttcacg cattgctcct gcccgctggc acaagaagtt
     1261 cgtgatacgt gacagctcga gctgtcaccg cacggctgat cgggcacgca gctctcgaca
     1321 caggctccat tggcacaaac gccctggctg caggcgattc cgttctgcac attgcccacc
     1381 ggcttgcaga cggcggtacc cgtgtcgcaa ctgagttgcc ccgccttgca ctccgtccct
     1441 ggcacgctgc aggccattcc gctggcgcag ggtacgcaag cgcctccgtt gcagacctga
     1501 cctgagccac agctggtacc gtctgcgagc gtggaccgtg cttcacagcg ctccatgccg
     1561 ctcgaacact cggtaaaagc ctggtccaca cagggattgg ggtgctcgca gctctcaccc
     1621 gcggcacatt gcgagggcgc gcccgccgtt ccgcctgtaa accctcccat cgcggtaccg
     1681 cccgcgccca tcccgcccgc agaagttcct cctgcagccg tacctcccga agccgcgcct
     1741 cctgaagtcg ctccgccagc aggggctcct ccttctgtgt tgccaccgct ggtgtgttgg
     1801 ccaccgctgt tgccttgagc gccgctgcct gcgccgccga gcgttccggc gaaacctccc
     1861 atctcgactg aactcgagcc gcccggcccg gctccaccgc cggaagaggc tccgccgcta
     1921 gaagtggctc cgccgccgga actccggccg ccagagctcc ttccgccgct cgcgtccgca
     1981 ctctgaccgc ccgaggccgc gggcttgcct ccactcgtcg aaccggggtt gtcaccgcca
     2041 gcgccgggtg cggtggggtc gcctcctccg ccgcaggcca gcaccagatt cgataacacc
     2101 agcaacgaat ggaaaactcg gtttctgatc atagcaattc gacacttcga tttagagacg
     2161 ggagttcgag ttcgagaacg caggcgcacc acatacctgc accggagcgc aggattcgct
     2221 gggcttcgcc caacttcgag ccgctactca ggacgctttt agcgattcgg agtccgcgag
     2281 tcacgcccca caggcgctgc cacacttctt ctgcgcgccg cgctgcggct cggcgggtgt
     2341 accactcaac tcgtaacacg tctgctcgac caactcgtta tcctgattga aagtattcac
     2401 ggttaccgtg tttaccccgc gcacacagcg gccccagtcg taagagatcc gctgcttcat
     2461 ccagctgtaa gtgtgatcga cacactccgg ctctccggct tcgtccttgt agcaagtgtt
     2521 ccagccactc ggcagctgcg tggaggcatc gtattcctcc atgtaactga gctcctgaag
     2581 cggaccaaag ccagtccacc agaggttctt gtgaccgtcc agatagctac cctcctcgct
     2641 gaggtaactc gcggagccgc cccaaggcag ggtaaacttt tggtaactgc cttccaacac
     2701 ccgatagtag ccccggcgtg acggttcgat tccgccgcga aacgtcggga tcgactcgag
     2761 attgccctcg aaatcgtata tttcccactt gccgcgctgc atgccgaatt cgtcgaactc
     2821 acccttcgct atggggatac cggccttcga ttcgaagcgc caatagcccg acggcttgtt
     2881 gccggagaac tcgcctgctt cgagcgtcac acccgtgtca ttgtcgacga gactatactc
     2941 gccctctaaa gcacccttcg agtagcactg cttctgagtc gcgttgtttc cgaaaccgat
     3001 gatctgagac tcgatacacc gagacggact cgcgccggag gtgaaagcct gctcgtggaa
     3061 gtaacaaaag tgagagtcct cgggtggcgt tgccgtgaga ctcgtcggac caccgtcgac
     3121 attccacgtc caggtcgagg acgagatggg cgaagactcg tcgaatttca ggtagtactc
     3181 gtaagctccc aaaaagctct cgtacggggt gccatccgca ttgatccgta ctccccactc
     3241 gaagggatgc agttcgaccg cgcgcacgta atccggatcg agcatcgagc taccggcggg
     3301 ggaaaagcgc gacggcccct gaacgagcgc ggtcgcagtg ccgggcttcc agtccaccgt
     3361 tagccacggg aaggagctca agcgggtgtt ggctccccct ttcatgtcga tcacgccggt
     3421 gccgttcgcg cgaaacgtga gacagtaatt cgaaccatac cagacgtgtc ccccggcttc
     3481 gccgtccgtc acggtgagtg ccttcagcac ggtgtagctg aagccctcga ttgcgccgct
     3541 cgtgaccgga taagcactat agattgcgcc gtccacttcg aacgagttgc cgattcctgg
     3601 gcccgggccg ggcatcagct tggcaactac gtagtggcta aaaccgccga tctgcgcgac
     3661 gaccgatgta ccctcgatac gggcgctcgt tacgggcagc aactcggcat cagcggactc
     3721 ccctcgatat aactttgcat ccgcgtcggc cgcgcgatct agctcgaaag acagagtcgc
     3781 cggagagtcg aatgaaagtc ctgcgggcga gaagtcgtac actgcgctca gcgcggtgta
     3841 cttttcgaga tccagctcgt accgatccac actgatctcg acctcctctt gcaaagcccc
     3901 gggcgcgatc tgcagcgcca tctttccgag cttcaattca ccgccggagg gcccgatcca
     3961 cgcgctcgcg tgatcttcgg tcgcccaccc ccccgtttgc tcggaacttc ctccggaact
     4021 cgagctgcca ccgctgctcg cgctcgtttc ctgcctcgcg ccgccgcgac tcgtttcttg
     4081 ggtggtgccg gcaccgccac tactggatcc aggggcgggg tcactcgaac cgttgcagcc
     4141 tgcggccgca aacgccatcg aaaacactgt aagggcgccg ctcactacct tgatggatct
     4201 caaaacggtt aatcctcgct gctattcgtc gcttcatcac tgcaggactg aagaactggc
     4261 aaacgagtaa gaacgccact tcagggtcgc catttaggcg atttattcag gcaaatgcgc
     4321 cccaccgctc gataccgtga gtcctatctt gatacgccct ggactgcgct cgcgaccgag
     4381 ggaacgggcg cgactagtcg aaagcaacac ccgattcgag cctacacgcc gtcccgtgat
     4441 cccctgtcac cgcgcaaacg ccacccgata tagcgacggc gacccattca ctctaactcg
     4501 tgcgcagtca cgactcgcgt gatcgcacgt ggcggttcaa ggccgggtta cgacttgcga
     4561 ctctacattg cagtagtcgc acttgttcca cgaatccccc cactctactc gcaccgaatc
     4621 gatgtccgtt cccgaggcgg tccgaaacga cggctgccac gtacagtctc cggcggtgtc
     4681 tttgtagggc ccaaaaacta cgtagttggc ggcgctgccg tcggcggggc ggccccgtat
     4741 atgcggcatg attttgacct tcacgccgcg accgttggtc cacgtatcgg ttatgcacat
     4801 ccagccgccc gtatcggagg ctccccaata aatccaaaga tgcacgatac ccgtttggcc
     4861 gtactccccg gtatcggggt gataaccgcg agtctgaatg tgacagaagc tcgccagctg
     4921 ctggcccacc atctctttcg agcagccgaa aggagccgaa ttggaagggg ctgcgggagc
     4981 ttgggcatag g
//
LOCUS       DASTKU010001007         5366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1314913, whole genome shotgun sequence.
ACCESSION   DASTKU010001007 DASTKU010000000
VERSION     DASTKU010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314913"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFQ61_35700"
     CDS             complement(<1..456)
                     /locus_tag="VFQ61_35700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959909.1"
                     /translation="MNSLAVVADPDTRERLRCMRILARQTPVCALGAANWGELQQTVE
                     EDSGIGLIFYTRSLPGAPADAIQQLLPHTGHLILAVEDGEQVPTAAGVTRVRRPLEEE
                     TLVLMARATGARSTSLRMSFMPVDFIQMICMSGDSQVLVLSHDGADVGII"
     gene            complement(493..1803)
                     /locus_tag="VFQ61_35705"
     CDS             complement(493..1803)
                     /locus_tag="VFQ61_35705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959910.1"
                     /translation="MKYSKCRTEAPSADEDARYADASNSAEFSNSAEASSAEASSAKA
                     SSAKSNSAKASNSPAQALPTVVAMSHNTDALQKVSVRTDVLALLREKRYEDALALLYR
                     ARSEAPADRELQKSIDQIKEFLVGAYAKRLGGLDRVATPIPSSAVRSPDVVLLSRYVD
                     GTSTFGDIAQMCPLGQLRTLQVLVGLYSMSSPPQLAGLSGRREPEPPLSGLRTPPEQT
                     NTLHTHGLSASPFVVHPAPQAIAETEDSAIIESDDLLTGSEEAMAEELGPPGTHTVVH
                     NSEPDDLPAPETARSASFASAAPLSAEENSEAVSAPPNAIRSAPVTMNPPVATREAPE
                     PGPQQPNSYQPSSYQPSSYQPGSYQPGSYQPGSRGPFSSQSGVESESTRQYNDSFAAA
                     TAAFVQGRYQEAVEAFEFCVRLRPGDRAAEVMLRRARRDFQSRS"
     gene            complement(4493..>5366)
                     /locus_tag="VFQ61_35710"
     CDS             complement(4493..>5366)
                     /locus_tag="VFQ61_35710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959911.1"
                     /translation="AWNTGRIFATALVVYIAGKLQEGGQTPKAAWGIAMGFAAAVMAG
                     FGIYHMYALPTGSIIRRPENAREIFTTFAETLTDFLKKPKLLGMLLFVFLYRSGEGFL
                     LVEAPLFMQAPLEKGGLGLSLSDKAFLDGAVSTGVSLAAGVLGGMFISRRGLKRSMMV
                     MALCMNIPHLCFVYLSHTVTPAAPLSLSTIAILVTLEKFGYSFGFVANMLYMMQQISP
                     GKYHMTHYAFCTALMNLVLIPTQASSGWIADHLGYKHYFIFVMIASIPSIIAAWFAPF
                     PHDEARGAKLGRAH"
BASE COUNT         1027 a         1573 c         1664 g         1102 t
ORIGIN      
        1 gatgatgccc acgtctgcac cgtcgtgact gagtaccaga acttgtgagt cgcctgacat
       61 acaaatcatt tgtatgaaat cgacgggcat gaagctcatc cggagcgacg tcgatcgagc
      121 ccccgtggca cgcgccatca ggaccagtgt ttcttcttcg agtggccgac gcactcgggt
      181 tacgcctgcg gccgtgggca cttgttcgcc gtcttctacc gccaagatca ggtgcccagt
      241 gtgagggagc agctgttgga tcgcgtcagc cggtgcgcct ggtaaagagc gcgtgtagaa
      301 gatcaacccg atcccggagt cctcttcgac cgtttgctgg agttctcccc aattggcggc
      361 gcccagcgca caaacgggag tttggcgggc aaggatgcgc atgcagcgca gacgttcgcg
      421 cgtatccgga tccgcaacga cggcgagact gttcattcgg cgaccaccag actgcaggct
      481 ctcctcacgc tttcaacttc ggctctggaa gtcacgccgc gctcgacgca gcatgacctc
      541 ggccgctcgg tccccggggc gcagccgaac acagaactcg aacgcctcca ccgcttcctg
      601 atagcgccct tgaacgaacg cagcagtcgc agccgcaaac gagtcattgt actggcgagt
      661 cgactcggac tcgacacccg attgcgagga gaatgggcct ctcgagccgg gttgatacga
      721 gccgggttga tacgagccgg gttgatacga gctgggttga tacgagctgg gttgatacga
      781 gttaggctgt tgcgggcctg gctcgggcgc ttcacgggtt gcgaccggcg ggttcatagt
      841 aacaggagcg ctgcgaatcg cgttgggcgg cgccgagacc gcctcggaat tctcttcggc
      901 tgagaggggg gccgcagacg caaacgaggc gcttcgtgcc gtttcgggcg cagggaggtc
      961 atccggttcg gagttgtgga ccacggtgtg agtgccgggc ggcccgagct cctcggccat
     1021 ggcctcctca gagcccgtca ggaggtcatc cgattcgatg attgcagagt cctcggtttc
     1081 ggcgattgct tgtggcgcag gatgcacgac gaaagggctg gctgacaacc cgtgagtgtg
     1141 gagggtattc gtctgctcag ggggagttcg caatcccgac aatgggggct cgggttctcg
     1201 gcgccccgac aatcccgcga gctgtggggg cgagctcatc gagtacagcc ccaccagtac
     1261 ttgaagggtg cgcagctggc caagtgggca catttgagcg atgtcgccga acgtcgacgt
     1321 gccatcaacg taacgagaga gcaacacgac gtctggcgac cgcacggcgg aagaggggat
     1381 aggcgtcgcc acgcgatcca gcccccctag ccgctttgcg taggcaccca ctagaaattc
     1441 tttgatttgg tcgatgctct tctgaagttc tcggtctgcc ggggcctcgg atcgcgcgcg
     1501 atacagcagg gctagcgcgt cctcgtagcg tttctcccgc aacagagcga ggacgtccgt
     1561 gcgtaccgaa accttctgca gagcatcagt gttgtgactc attgccacga ccgtaggtag
     1621 ggcctgtgct gggctgttac tggccttcgc gctattggac ttcgcgctac tggccttcgc
     1681 gctactggct tccgcgctac tggcttccgc gctgttactg aattccgcgc tgttactagc
     1741 gtccgcgtag cgagcatcct catcggcgct gggagcctct gttcggcact ttgagtactt
     1801 cacgttaggg tttacttctt taagagtggc tttcgcggga acgcgcggtt tcatgctacg
     1861 gctttgccca cagctcagcc cagagcggga gcgcgagcac cgaatacacg tccactttcg
     1921 tgagcaattc agcgcaattt ggctggaagc ggcgactcac gtacgcgcta cacacaagga
     1981 atgaaggccc aggaactcga acaaagcagc cagaacgtgg agcaggcacg gcgcagacca
     2041 ccgcgtctcc aacaagccca gtttgtgcaa ccctgaggtc caagtggtga ctgctctaca
     2101 gacccggcgc attcggtcgc cgcgaagata acaccagcgt ataaacaata ctccatctcg
     2161 gttggttcac gctagaggca tttgtgacga tttgaatcga cagtggcctg ggttgccgcg
     2221 ggtcacgcga acggcagtgc tgtgctggct ctgctttggc aggtgctggc tctgctttgg
     2281 gcaggtgctg ggtctggggc tcgcgggcag aacgctccag atcattgaaa gccaatgctc
     2341 tgagcagggg tgtgtgttca gaagcgtttc agatgggctt caagcggaga ttgaagcgtc
     2401 gagtccaaac gcttatcggg cgtgaggcca ttcgttctgc cggtccggca ccagtagacc
     2461 ttccgttggg agactttccc ttgggagacc ttcccttcga agtcgcctcg ctgttctgac
     2521 acttcggtcg accgcttttc ttcgagtcaa cgcgactcgc gccgctcaca cgctactccc
     2581 gcgggctggc gcgccgggcc cgggtttggt gctggttttg gggcttttcc ggtgcgatcg
     2641 gtgcgcttga cgctgcaaag ctcacgagta cagcttaggt aagtgacagc gctcgagcca
     2701 agcgctttga gccgagcgtt tgagccaagc atttggctcg aacattgatc caaacagttg
     2761 accgagtagt taaccacgcc aacaggattg gtggcatacc accaggagga gtggtggccc
     2821 cgatgcggtg taaccaggcg tgcgcgtagg cgatcggcgg catctgcagt tttcgagctc
     2881 gtaactgccg atcactcaaa tcgaaacgta tcagaagaag ccccggactc cggggaccaa
     2941 gctccatcca gcgctttgct ttcagcatat cgcctccagg caaaacgagg ttcgaggtcc
     3001 aaggtaccgt gagttattga agatcgcgca acacgactgt ccccagttgg ccccgtagaa
     3061 tcacgagagt tagtgtgttg catttgtgcc ggtccgtgca gctgtgggtt gtgcccatgg
     3121 gaacgggccc cgtgcggcag cacccaagca gaaacaaacg ttatgtagac tcgaaacgag
     3181 aggctgcggg actcactcct cgcgatcggc ggctcgcgac gggcgtcttc ttcaaacatt
     3241 ggtccggagt gccggtgtcg tgccattcag cagccgtatc gatcgtacga ggtgtaaccg
     3301 ggctgtgcga agaggtcccg cgcggtgccg cttggctgtc ggggtgcgag tatcggatcg
     3361 ccagctgcac ttcgtgtccg gctcccaaag tgcgcttcgt gtgtgcggcg gttctagtcc
     3421 cgtggcgacg ctcttctgtt ttatcttcgc tggactcgta gttgtcactg ctgttctttc
     3481 ccctcgctac gctgttcttt cccctcgcta cgaggttctt tcgccgcgcg ctgttctttc
     3541 ttatctgcgc tgtcccctcc atcgctgcga ggttgtatct gtcgctgagc ggtccatgtg
     3601 agtggtaggc tgcgctttgt gcgtctcgcc ggtgcgcttt ggggttggtc ggctgcgctt
     3661 cgtgtgtcga gcggcctgaa gccggggcca ctcgtgtgca atggagtaga ttaagtgcaa
     3721 agcccgctcc aagcacgaga gctgcgtggg ccgcactctc cttctcgagc aggccagtaa
     3781 cggacgccgc cgcatcggca gttcgccaat tcaggaccgg caagtcgcgc cgcgcaatag
     3841 cttccaatgt gctctggtgc cttgctcgca cgctgtagcg tgacgtgctc gtcgagcatc
     3901 gccaattgct gccaggcggg cgttgagttt cacttggcag attccgattt ggacacccgc
     3961 ccgccaggac taggactaga acgccgaacg gctgaaacca gcagcagaaa caccgagacc
     4021 gaagcgagag gtcgcttgcg cgagcgcctc atccaagcgg tcgtgaacat ccttgcgcta
     4081 atcccagggc acgtggaacg aaacgaaagc cccctgcaag agctcgtgtt cgccgctgta
     4141 gatcgccgct gtagaatgat agactctgaa tctcatcgaa caggcatgtt cgagcttccc
     4201 agccagcctg gcgacagggt gacagtgcag cgccggacat tgttgtccct atctcacatg
     4261 cggtgcacta gtggtttcga cccttcgaag gacgcttcgg caggcggtgt cggtatgcca
     4321 gctggcgatc cctgaaccgc gaatcggggc gcggatttca tcaagggacg gagcaagctc
     4381 ccgcacggct tgcgccgttt gcttttctcc cgcgctgcgt cgggcgctgg ggccgcagtc
     4441 gccagcctat actggcgact gctaacgaat cgtagtgaaa ccgctacgtt gctcagtgtg
     4501 cgcggccgag ctttgcgccg cgagcttcat cgtgcgggaa cggtgcgaac caggcggcaa
     4561 tgatcgaggg gatactcgcg atcatcacga agatgaaata atgcttgtaa cccaagtgat
     4621 cagcgatcca cccgctcgaa gcctgggttg gaatcaacac caagttcatg agcgcagtgc
     4681 agaacgcata gtgcgtcata tgatatttac cgggcgagat ctgctgcatc atgtacagca
     4741 tgttggcgac gaatccgaag ctgtaaccga acttctcgag tgtcaccaag atcgcaatcg
     4801 tggagagcga gagcggcgcc gcgggcgtga cggtgtgact caagtacacg aagcacaggt
     4861 gggggatgtt catgcacagc gccatcacca tcatcgagcg cttcaagccg cggcgcgaga
     4921 tgaacatgcc gccgagcaca cccgccgcca gactgacgcc ggtgctcacc gcgccgtcca
     4981 agaacgcttt gtccgagagg gacaagccga ggccaccctt ctcgagcggt gcttgcatga
     5041 acagcggcgc ctccaccagc aggaagcctt caccgctgcg gtacagaaac acgaagagga
     5101 gcatgccgag caacttcggc ttcttgagga agtcggtcag ggtctccgcg aaggtggtga
     5161 agatctcgcg cgcgttctcg ggccgacgaa tgatcgatcc ggtcggcaaa gcgtacatgt
     5221 gataaatgcc gaagcccgcc atcaccgccg cggcgaagcc catcgcaata ccccaagccg
     5281 ccttcggagt ttggccgcct tcctgcaact tgccggcgat gtaaacgacc aaggcggtgg
     5341 caaagatacg cccggtgttc caggct
//
LOCUS       DASTKU010001008        16353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1760763, whole genome shotgun sequence.
ACCESSION   DASTKU010001008 DASTKU010000000
VERSION     DASTKU010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 16353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16353
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760763"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFQ61_35715"
     CDS             complement(<1..187)
                     /locus_tag="VFQ61_35715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959912.1"
                     /translation="MSRYAGPWFSLPPLATFRAEWTIGRGFLDRSPCRARRWSQPGAP
                     RQVYSEAMRDTSPEAEER"
     gene            complement(597..953)
                     /locus_tag="VFQ61_35720"
     CDS             complement(597..953)
                     /locus_tag="VFQ61_35720"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HET9959913.1"
                     /translation="MATVRMVRQSERSVFVDLLFASSGIEPEIVNSAEEIELLDGITL
                     AVATRAHLIALKVLARDDRNRPQDWDDLRVLLADASPSEVDEARDALRLIELRGFNRG
                     RDLSADLSRTIAEFAK"
     gene            complement(979..1206)
                     /locus_tag="VFQ61_35725"
     CDS             complement(979..1206)
                     /locus_tag="VFQ61_35725"
                     /inference="COORDINATES: protein motif:HMM:NF040165.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959914.1"
                     /translation="MAQQTSQTARENVKTALELLDVAERMLRRRLRREHPHLTEEAIE
                     QRIVDWYQHRPGAELGDGMGVIGPWPRQRTQ"
     gene            complement(1539..2108)
                     /locus_tag="VFQ61_35730"
     CDS             complement(1539..2108)
                     /locus_tag="VFQ61_35730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9959915.1"
                     /translation="MSSPAAQRTYTFREYVELDTSSELRYEFFAGAIYAMSGGSPEHS
                     GIAANIIQLLGVQLAGKPCRVFTSDLRVRVLDTGLATHPDVTIVCGALARDPEDSHTV
                     TNPVVIIEVLSPTTERYDREEKFAHYRRIQSLASYVLISQEERRVEVFTRNSDGSWTL
                     RETRVGAAELPAIACSLEVADVYRDPLAS"
     gene            complement(2394..2684)
                     /locus_tag="VFQ61_35735"
     CDS             complement(2394..2684)
                     /locus_tag="VFQ61_35735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959916.1"
                     /translation="MQLAGSRAASSPRTVGGLEGSEPTDILWFGVPPTRVDVLRRIDG
                     VDFDQAFEKRVIAEWEGVPVCVIGRDELIAAKRAAGREQDLLDVRALTRLKR"
     gene            3210..4583
                     /locus_tag="VFQ61_35740"
     CDS             3210..4583
                     /locus_tag="VFQ61_35740"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET9959917.1"
                     /translation="MASSGGQQSGGAATANGGTVASGGVAASGGQQSGGAATANGGTV
                     TSGGAVASGGQQSGGAATANGGTVASGGAVASGGQQSGGAGTSGSPGSGGSTSITPGK
                     QGERFPFPQNVKYPYGVKSTKVTNDFVRSWYENWKKTRLVSCNGNLMPGTDNSSTAKV
                     EAQGFAMVAVAYMGDKDVFDKLYSFYLTKTSSAACGLSGWQTSCGGVTDPGAATDGDI
                     DVASGLVVAHWQWPDAGYDAKAKTLIGNLKKMLLDCSGKWTVYPGCSGGKPWGGCNET
                     DISYYSPAFFRYFAKISGDSTWTKLADDTQKIRDAAANSSTGLVPDWQSVSGTAGAGS
                     RAGNYGFDAIRAPYKQCLDYLWNGTPEALTWCKKISSWANGVGVTKITDGYQLNGSPS
                     SNNHNMASVGSLTIAAMANTQEMADAFVGESVKMKDDYWYSSYLGNLYLLAMSGNMWN
                     PDILGVQ"
     gene            4738..5631
                     /locus_tag="VFQ61_35745"
     CDS             4738..5631
                     /locus_tag="VFQ61_35745"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: protein motif:HMM:NF006186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HET9959918.1"
                     /translation="MVSTIFKQTRWTAIDRFDFKDITYHRAIEQGTVRIAFNRPEVRN
                     AFRPQTVDELATALEDAHTLTDVGCVLLTGNGPSPKDGGWAFCSGGDQRIRGKDGYKY
                     EGAAGGADLGRLGRLHILECQRLIRFMPKVVIAVVNGWAVGGGHSLHVVCDLTIASRE
                     HGKFKQTDPDVASFDSGYGSALLARQCGQKRAREIFFLGKTYSAEEAFQMGMVNAVVP
                     HAELEDFALEWGAEINKKSPTAMKMLKYGFNLPDDGMVGQQLFAGEATRLAYGTDEAE
                     EGRDAFLEKRKPDWSRFPWRY"
     gene            5943..7586
                     /locus_tag="VFQ61_35750"
     CDS             5943..7586
                     /locus_tag="VFQ61_35750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959919.1"
                     /translation="MRFAYLALAGSNALSIGSAILITSSPGLAQQNRGVAETVAQSSR
                     SESPRAAPEREPIVVVVHSDTPSLGATQIREAVAQELGQQVLGPEDPRAGSLSRRLTI
                     SHRASRRELAVSYSDPSRGNWTRVIESPESISDVVSASALLAGNLVRTEADSLLPPAP
                     PAPPPAPPDPLPPGNNRNSTGFAPTELASGGPPGPESPRPQQLASAAFFYPLATNYGQ
                     PEIETNFEFNLLYGRVGKLEGVGIGSVNVASDSVRGLSIGLANVVGHSVGGLQLAGLF
                     NLSGESVQGLQVAAGLNVARGPLEGIQASAIGNFAGAPSSGAQLAFGINHAPAFEGLQ
                     VAGVANLNLGYMQGVQVAPVFNYGGNIKGLQLGLVNVAKRVRGAQVGLINVADDVEGA
                     PIGLVSVTRTGGIHPQVWAASSSYFNAALKFATRYTYTFLSFSVHENDGHRLQGPGFG
                     VGFTVPAIPRLFFDTDLSGTHLFGKGACCGESFFSSVSRERDWTLFRLRAHLRYEFLP
                     HLSVFAGGALGARLTYPLDEDDNDIRGRSLLEGHAGVQL"
     gene            7594..8196
                     /locus_tag="VFQ61_35755"
     CDS             7594..8196
                     /locus_tag="VFQ61_35755"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9959920.1"
                     /translation="MSLWYKAGVLSRPLTRESPDGDEDALLAELAQGSERALRTVYRH
                     HHEAVRALARRLLGHEADAEELVQEVFVSLPAAMRGYRGQSSLRSFTLGITTNHARHF
                     VRAAARRRSAVHRFAHEPKSELAPAADERVVREQLAERLMCAMDQLSHDQRVAFVLCE
                     VEERSSEEAAAILGIPSSTIRARLGAARQKLQSLLSEEWP"
     gene            8193..9002
                     /locus_tag="VFQ61_35760"
     CDS             8193..9002
                     /locus_tag="VFQ61_35760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959921.1"
                     /translation="MKADPLEDAVSALRERYDGQSDAASRSEDAIIGRMRHKTRQRQR
                     ARFAMPFAALLAASSAWAARDSISESVRQVGAWLRHESAQSSGLNQRGGQPRSHEQPR
                     DQGEGAERPKPSRPQPEELAPHSPSSAPEGAPNTLPREPSAPTAKPRSTPSPRNSTAP
                     TARAQNAPSPQLGEHPAKPDAMTLYREAHRLHFLDKNPEQALLAWDRYLARGQGDFAI
                     EARYNRALCLWRLGRIAEAQSELRPFARGDYGGYRQAEAAALLSNSAAPHP"
     gene            9110..9763
                     /locus_tag="VFQ61_35765"
     CDS             9110..9763
                     /locus_tag="VFQ61_35765"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HET9959922.1"
                     /translation="MAKSKIPNPDSGLAPLTHNPFAKLAASQSPTPPGTPAAVAAVAA
                     KAPPQERARERTRAITARLETRGRSGKVITRIAGLPTPALALIASRLRQALGCGGSLE
                     GEDLILQGSLAERATAWLQSTDLSALTREATQPKPKIVEPAAPPSSPVALASGIHRRD
                     VRPGKRVAVVQKADQESGELTFGIVRDILTSSPTHPRGIKVRLQTGEVGRVKAVYDA"
     gene            complement(9795..11375)
                     /locus_tag="VFQ61_35770"
     CDS             complement(9795..11375)
                     /locus_tag="VFQ61_35770"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9959923.1"
                     /translation="METEVIEGQLIADKYRIERVIGHGGMGYVMAAQHLALGDRVAIK
                     FLWPEALQNAEAVARFEREALAAARIKSEHVVRVFDVGRLDSGAPYMVMDYLEGEDLE
                     TWLEQEGMFPVEQAVDFVLQACVAVAQAHSLGIVHRDLKPANLFCVQRSDDELIIKVM
                     DFGVSKLSDDVRIPGLLRMSVTKTDVVLGSPLYMAPEQVKNAKDVDARCDIWGIGVVL
                     FELLTGRVPFGGDSFGEVAINIATEPVPALSNYRADVPTGLQEVIARCLQKDRRQRYP
                     DVAMLARALLPYASAQGVSLAERIGRILGTTHDTARKVLADTGSQRSEAHSAPGAWES
                     VLEVAAPHSGAPLESSSSVPILAKWYSNEKLSARRSAIRTVAVFALTFAVIGAVAWSG
                     TPYADTARGRQQLSGGDVSSLASPAIDRALADPVVSPVVPSSSAANVSISARPSVARR
                     SKAGMRNPAQGRQVGTAALGSVVGAVATAPIAPSEGSAATPPAPPQPGEDLHKTLGPP
                     ELRALDEVDPYPQQRGGD"
     gene            complement(11924..12475)
                     /locus_tag="VFQ61_35775"
     CDS             complement(11924..12475)
                     /locus_tag="VFQ61_35775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959924.1"
                     /translation="MATLSTTAWVLHDLGLAAGFGGNLFGQLALNPAVKVIDSKEQRS
                     KVSHAAWDRYKTVNAIALAATAGTWLIGRAALSGREVGQTSRELTLVKDVLVLGAFTT
                     GVGALVTGTLLDNELTRPVPIESGHYAAPETPPRVARLQKIVNVVGALNVAFEAGVLA
                     LTTVLAMKAGKSSKWSLVSQLLP"
     gene            complement(12632..14200)
                     /locus_tag="VFQ61_35780"
     CDS             complement(12632..14200)
                     /locus_tag="VFQ61_35780"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET9959925.1"
                     /translation="MEHLTFTSLQTASRASIALTLGLTLSACSSDAEPQRRPEETEIP
                     ARFRAFAERFERERRALGIPGAAVAILEHGQLSFARGFGTKGINSTEPVSAKTIFRTG
                     SMGKVITAIGAMSAVEDGLLDLDAPIRAAIPDLSLASPEAESLTLRVLMSQQSGLRDY
                     IQLPGPAEDSGLAEFAEGAELAENVDFINPPGLFWNYSNPNFYIAGRALERSTGVSYR
                     EAIRERVFAPLHMDRSFFLASDVLADGDYSHGYGINSVSSEVTDYEDISPDSYENTWA
                     RPAGLGFSNVLDWARLMQFLMRGDPNVLSELSRHEIVSSQISTHTIYSDVHATALGLA
                     DDYGLGVGVSQGFFMDHQSEPNTYYPVPFIGHGGDISGFASTFAVFPSTGFGIVVLSN
                     RDIERPVESIRLALESFGGLPPPSAPPAGREVDPSRLEQYAGTYRDAQGSSVMLRAER
                     GALLVSGPLLDSTQLPYEPKLQPTSLDNFYLWVTVQGQRLPLEVTFFPDEAGQYTWFR
                     SRLAVAQRADSERE"
     gene            14354..14791
                     /locus_tag="VFQ61_35785"
     CDS             14354..14791
                     /locus_tag="VFQ61_35785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959926.1"
                     /translation="MRSRSFAARQVGNVVIYVHTSEPPSDEEWDRVLSFYEGAASTLE
                     PKILVYTEGSAPNAAQRARLNGVFGKRRVLLAILTSSALARAAGTAVHWFRPEVRVFG
                     AREFPKAAMHLGLTPAEATAIESALNELKGELQLGAPAPTRER"
     gene            complement(14889..15329)
                     /locus_tag="VFQ61_35790"
     CDS             complement(14889..15329)
                     /locus_tag="VFQ61_35790"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET9959927.1"
                     /translation="MTEALLEFLVHYGFDSRDLNAPGAYGLTPLMRACLIGSGPLVNE
                     LLELGVDVNLRNMDGNTALWLACVSNSGEIVRALARAQADLDHRNDVGASALMYAASS
                     GKAPIVALLLELGANPHITNVDGAKAGDMAATLECLKLLRYSVV"
     gene            complement(15326..>16353)
                     /locus_tag="VFQ61_35795"
     CDS             complement(15326..>16353)
                     /locus_tag="VFQ61_35795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959928.1"
                     /translation="SVSARELRERAATATFHGRASVIDAHPMYTWPIVESTAVLTRFR
                     DEEFPQGRDVAWGGPVAPPRLVASSDHPLAAAQLVQGRRSAQRFNAGFVLARQAFERL
                     LKAASCPYSPSASDSGPPGTPLDGEVATQIHLVLFVHRVDSLEPGIYLVRRAGEPSDA
                     GLGEPEPGEPEPGEPELGEPELGKPRLDVSEKPSGLLPCFEAHFPVRRVHDSSPELEL
                     FQVSPCASRELARISRLLHCQQEIAASCCFAVAMLADVARAAELCPAAYRDLHREAGR
                     IGHSLYLQAELEGVRGTGIGCFLDEPVAEFLGLQNSPYRSIYHFSVGQAQLDPRIETA
                     AVEAEDT"
BASE COUNT         3145 a         5248 c         5083 g         2877 t
ORIGIN      
        1 agcgttcctc ggcttcgggt gacgtgtcgc gcatcgcctc cgagtatacc tgccgcggag
       61 cgccgggctg agaccaacgc cgcgcccggc agggcgatcg atcgagaaat cccctcccaa
      121 tcgtccactc ggcacggaac gtggccaacg gcggcaaact gaaccaaggg ccggcatatc
      181 gcgacatctg gcgggtgcca tgtacccaga gctggaggga cagaactgcc gagcggtcgc
      241 gccgaaccgc tcattggagc taggggccag cgcgcacagc ccaacgctcg tcgcggggaa
      301 cgtttgcgcc ttactcccgg atccccgaac tcggcttccc gggactgaat cgccaaggcg
      361 cggcaaagta gaaccgctcg ttgcgtcctc tagttccgct ccacccgtgg gcttgaccac
      421 gggctttgaa tcgtctccga atcgtccacc gcgcgtaccg aaacgtcgcg agcgggagtg
      481 aactggaccg cgaaccggct gtttcgcgac atctcgcagc atctcgatac gtgctaaccg
      541 gatttaggtt ccggcgccgc aaggcgactc cctcctcgtg cacaagcagg ttgcgattac
      601 ttcgcgaact ccgcgatggt cctcgacaga tcggccgaga gatctcggcc gcggttgaat
      661 ccgcgcaact cgatcagacg caaggcgtcc ctcgcttcat cgacttcgga tggtgacgca
      721 tccgcgagaa ggacgcgcag atcgtcccag tcctgagggc ggttgcggtc gtctcgagcg
      781 agcaccttga gggcgatcag gtgagcgcgc gtcgctacgg ccaacgttat cccatcgaga
      841 agctcgatct cttccgcgct gttcacgatc tcgggttcga tgccagatga cgcaaagagt
      901 aggtcgacga acacggagcg ttcgctctgc cggaccatgc gaacggtggc tatgcgtccc
      961 gtacggttag agcgcgctct actgcgttcg ctggcgaggc catggtccga ttacgcccat
     1021 cccatcgcct agctccgcgc cagggcggtg ctggtaccag tcgacaatgc gctgctcaat
     1081 cgcctcttcg gtgagatggg ggtgctcccg acgcaggcgc cggcgaagca ttcgctcagc
     1141 gacatccagc agctccagag ccgtcttcac attctcgcgg gcggtttggc tggtctgctg
     1201 tgccacgggg agagcgtatc acggcgtggg acacacggcc gcgcgcggaa gccctgcggc
     1261 gggggccgag cccgtggggt ttcgtccgcc ggttccgggg actcgcgacg gcgggcaaac
     1321 gtctccgaat cgtctcccgg ctgaaccgag acgccgcgaa cggtcgcgaa ctggatcgaa
     1381 agccggccgt ttcgcggcat ctcgcggcat ctcgatacgc gagagctgga tttaggttcc
     1441 ggcgccgcaa ggctcaagtc cctcctcgtg cacgacggtt atcggggact tcgtccccgc
     1501 cccgctgaaa acgagatcag cgggaccact gccaaagctc agctcgccag cggatcacga
     1561 tacacgtcgg ctacctcgag cgagcacgcg atggccggca gctccgcggc accgacacgg
     1621 gtctcccgta gcgtccagct accgtccgaa tttcgtgtaa acacctcgac gcggcgctct
     1681 tcctgggaga tcagcacgta gctcgcgagg ctttggattc gccggtagtg ggcgaacttc
     1741 tcttcgcgat cgtagcgttc cgtcgtcggg gacagcactt caatgatgac gaccgggttc
     1801 gtgaccgtgt gcgagtcctc aggatctcgc gccaacgccc cgcaaacgat ggtcacatcg
     1861 gggtgcgtag ctagtccagt atcgagcaca cgcaccctca gatccgaggt gaagactcgg
     1921 cacggcttgc cggcgagctg cacaccaaga agctggatga tgtttgctgc gatgccgcta
     1981 tgctccggcg agccgcctga catcgcgtag atcgcgccgg cgaaaaactc gtagcgaagt
     2041 tcggagctcg tatcgagctc gacgtattcg cgaaaggtgt acgtgcgctg ggcggcggga
     2101 gaggacatct cgcccgaaga ctagcatcgg acgcctcgat ggtcggctgc cccccgtccc
     2161 ccctccgtgc atgagcactt cgtccacgtt ttccggcgtt cgaggggcaa gcgaatcgcc
     2221 gcccgaatcg tctaccgatc gtccaccacg cgtatcgaga tgtcgtcaac ggtagcgaac
     2281 tggatcgaaa gccggccgtt tcgcggcatc tcgcggcatc tcgatacgcg agagctggat
     2341 ttaggttccg gcgccgcaag gcgtgggttc gactccctcc tcgtgcaccg cagctagcgt
     2401 ttcagacgag tcagcgctcg gacgtctagc aagtcttgtt cgcgaccggc ggcgcgtttt
     2461 gcagcgatga gttcgtcgcg accgataacg cagaccggaa cgccttccca ctccgcgata
     2521 acacgcttct cgaaggcttg gtcgaagtcg acgccatcga tgcgacgcag aacgtccact
     2581 cgggtcggtg gaacgccgaa ccagaggatg tctgtcggct cgctaccctc gagcccaccg
     2641 acggtccgag gtgaagacgc ggcacggctc cccgcgagct gcattccaag aagctggatg
     2701 atgtttgctg cgatgccgct atgctcgggc gaaccgctga cgtcgctaga tcgcgcccgc
     2761 gaaaaactcg tagcgaagtt cggaggtcgt gcacgaaggc ggacggtgtt cgccggtacg
     2821 aaccgagagc aggagacgtt taatgccaag cccgggatgc tggacaaccg agtcgcaagc
     2881 tggcggcgtg tggtcgaaca gacggcgatc cgtcgaacct tctctccgaa aaatgacttt
     2941 ttaccgaggg ttcttggttc tccgtcacga cgcggcgaat gcgagttgcg ctcgtgctcg
     3001 ccgacgccaa gatgccgggc atgacacttg tccgagtagc attaggaagc atctggctta
     3061 gcctctcgct tgcagcttgt ggccaatccg ggggagtagg cgattctggg aaggccggag
     3121 cgagcggcaa cgcaagcggc ggagcaacca ctgcatcgac aggtggatca ggcggcggct
     3181 caaagggtgg aacggcctct tccggaggcg tggcctcctc gggcggtcag cagagtggcg
     3241 gtgcagcaac cgcgaacgga ggcactgtcg cctccggagg cgtggccgcc tcgggcggtc
     3301 agcaaagtgg cggtgcagca accgcgaacg gaggcactgt cacctccgga ggcgcggtcg
     3361 cctcgggcgg tcagcaaagt ggcggtgcag caaccgcgaa cggaggcact gtcgcctccg
     3421 gaggcgcggt cgcctcgggc ggtcagcaaa gtggcggagc cggaacgagc ggatcgcccg
     3481 gctccggcgg atcgacctcg attacgccgg gcaagcaagg cgagcgcttc ccattcccgc
     3541 agaacgtgaa gtacccgtat ggtgtgaagt cgacgaaggt caccaacgac ttcgtccgga
     3601 gctggtacga gaactggaag aagactcgcc tcgtcagttg taatggcaat ctgatgcctg
     3661 gaaccgacaa ctcttcgacc gccaaggtcg aggcccaagg gttcgcaatg gtcgctgtgg
     3721 cttacatggg cgacaaggac gtcttcgaca agctttatag cttctacctg acgaagacca
     3781 gcagtgccgc atgcgggctc tcgggttggc aaaccagctg cggcggagtg acggatccgg
     3841 gtgcggccac ggatggtgac atcgatgttg cttcgggatt ggtcgtggcg cactggcagt
     3901 ggcccgatgc gggctacgac gccaaagcga agacgctcat cgggaacctg aagaagatgc
     3961 tcctcgactg cagcggcaaa tggacggtgt acccgggctg ctcgggcggc aagccttggg
     4021 gcggctgcaa cgaaacggat atttcgtact actcccccgc gttcttcagg tacttcgcca
     4081 agataagcgg ggacagtacc tggaccaagc tcgccgacga cacccagaag atccgtgatg
     4141 ccgccgccaa ctccagcact ggactggtgc cagattggca gtcggtgagc ggcacggccg
     4201 gtgccgggag cagggctggc aactacgggt tcgatgcgat ccgcgcgccg tacaagcaat
     4261 gcctcgatta cttgtggaat ggaacgcccg aggcactcac ttggtgcaag aagatctcga
     4321 gctgggccaa cggcgtcggc gttaccaaga tcacggatgg atatcagctc aacggttcgc
     4381 cctcgagcaa caatcacaac atggcctccg tcggcagtct caccatcgcg gcaatggcca
     4441 atacccaaga gatggcagac gccttcgtcg gcgagtccgt gaagatgaag gacgattact
     4501 ggtacagcag ctatctcggt aatctctact tactggccat gtcgggcaac atgtggaatc
     4561 ccgacatctt gggagtgcag tagctttcgc ccagacggtg aagcacgccg agatccgtcc
     4621 ggctcttggc gtgcttctcc cgtacttgcc aacgcggtgt gcctccggag ggacgttgcc
     4681 gtctcggagg cgcgttggca gcttgcgcgt tgcgacgaga ggggcgaaca tcgccgcatg
     4741 gtttcaacca tcttcaagca aacgcgctgg accgcgatcg atcggttcga cttcaaagac
     4801 atcacttatc atcgcgccat cgagcagggg acggtccgga tcgcgttcaa tcggcctgag
     4861 gtacggaatg cttttcgtcc tcagacggtc gacgaactag cgactgcgct cgaagacgct
     4921 cacacgctga ccgatgtcgg ctgcgttctc ctcaccggca atggtccctc gcccaaagac
     4981 gggggctggg cattctgctc cgggggtgat cagcgcatcc gcggtaaaga cggctacaag
     5041 tatgaaggcg ccgcgggcgg cgcagacctc ggtcggctgg gacgcctgca tatcctcgag
     5101 tgtcagcggc tgatccgctt catgccaaag gtcgtgattg cggtcgtgaa tggctgggcg
     5161 gtgggcggcg ggcacagcct gcacgtggtg tgcgacctga ctatcgcgag tcgcgaacac
     5221 ggtaagttca agcagaccga tcctgatgtg gcgagtttcg atagcggcta cggctcagcg
     5281 ctgctggcgc gacaatgcgg tcagaaacga gcgcgggaga tcttctttct tggcaagacc
     5341 tacagtgccg aggaggcgtt ccagatgggg atggtcaatg ccgtggttcc gcacgcagaa
     5401 ctggaggact tcgcactcga gtggggagca gagattaaca agaagagccc gactgccatg
     5461 aagatgctga agtacggatt caaccttccc gacgatggga tggttggaca gcaactcttc
     5521 gccggcgaag caacccgact cgcctatggc acggacgaag cagaggaagg gcgcgacgct
     5581 ttcctcgaaa agcgcaagcc ggactggtcg cgattcccgt ggcgctactg aatcgacctc
     5641 ccgagttgac cgccgcccgc gcccgtaacg atggctgctc gtgtcgcgaa gcgcctcggg
     5701 cagggcggtc gggcgggtct ggcgatggcg gcgctcgctt tcgccgtttc tattcggata
     5761 cgccgtaagc agctcggata gtccgctcct cggcgaagct gggacccaag gcagccgccg
     5821 gtccttgccg gtcgttcgga gcgaagctcg cggcagaggc tcggtccaaa ggctcggtga
     5881 aaaaagtgcc ctgattctct ccgcgcagtc gtcagagtgc ccgcacccgg gcactgaggg
     5941 gcatgcgctt cgcttatctc gctttggccg gatcgaacgc tctatcgatt ggcagcgcca
     6001 ttttgataac cagttcgccg ggtctggctc aacagaatcg tggtgtggcc gagactgtcg
     6061 cccaatcgtc tcgctccgag tcgccgcgag cagcgcccga gcgtgaaccg atcgtggtcg
     6121 tcgttcattc cgatactcca agtctcggcg cgacccagat tcgcgaggcc gtggcccagg
     6181 agctcggcca gcaagttctc gggcccgaag acccccgtgc cggttcgtta tcgcgacgcc
     6241 tgaccatctc gcatcgagca tctcgaagag agctcgccgt cagctattcg gatccttcgc
     6301 gtggaaactg gacccgcgtc atcgagtctc ctgagtcgat ctccgacgtg gtgagtgcca
     6361 gcgcattgct cgcgggcaac ctagtccgca ctgaagccga ctcgctgttg cccccggccc
     6421 ctccagcgcc gccccccgcg ccgccagatc cgcttcctcc cgggaacaac cgcaactcga
     6481 cgggattcgc gcctaccgag ctcgcctctg gcggccctcc tggccccgag tctcctcgtc
     6541 cgcagcagct ggccagcgcc gccttcttct accccttggc caccaactac ggtcaaccgg
     6601 agatcgaaac caacttcgaa ttcaatctgt tgtacggccg cgtgggcaag ctcgaggggg
     6661 taggcatcgg gagcgtgaat gtcgcgagcg atagcgtacg aggtctatcg atcgggctcg
     6721 cgaacgtagt cggccattca gtcggagggc tgcaacttgc aggactattc aacctgtcgg
     6781 gtgagagcgt gcaagggctg caagtcgcgg ccggtctgaa tgtcgcacgc gggccgctcg
     6841 aaggcattca agcctctgcg atcggaaact tcgccggtgc accctcgagc ggcgcacagc
     6901 tcgccttcgg aatcaatcat gctccggcct tcgaaggcct ccaggtcgcc ggcgtcgcca
     6961 acttgaatct cggctacatg cagggagtcc aagttgcgcc tgtgttcaac tacggcggca
     7021 acatcaaagg ccttcagctt ggcctggtga acgtcgccaa acgcgtacgc ggagcccagg
     7081 tgggattgat caacgtcgca gacgatgtcg agggcgcacc gatcggtctc gtcagtgtga
     7141 cgcgcacggg cggcattcat ccccaagttt gggcggcgtc cagcagctac ttcaatgcgg
     7201 ccctgaagtt cgcgacccgc tacacctata cgttcctgag cttctccgtc cacgagaacg
     7261 atggtcatcg tttgcagggc ccaggatttg gagtgggctt caccgtacca gcaataccgc
     7321 gcttattctt cgacacggat ctcagcggta cccacttgtt cggcaaaggg gcttgctgtg
     7381 gtgagagctt cttcagctcg gtctcgcgcg agcgggactg gacgttgttc aggttgcgtg
     7441 cgcatctgcg ctacgagttc ttgccgcacc tgagcgtatt cgcgggcggg gcgctgggtg
     7501 cgcggctcac gtatccgctc gacgaggacg acaacgacat tcggggccgt tccctgctcg
     7561 agggccacgc cggtgttcaa ctctaaaggt gtcatgagcc tatggtacaa ggccggcgtg
     7621 ctgagccggc ccctgactcg agaaagccct gacggcgacg aagacgcgct cctggccgaa
     7681 ctcgcccaag gttccgagcg agccctccgt acggtctacc gacatcatca tgaggccgtg
     7741 cgcgcactcg cgcggagact gctcggacac gaggcggacg ccgaggagct ggtacaagag
     7801 gtgttcgtga gcttaccggc cgcgatgcgc ggctaccgcg gtcagtcgtc gctgcgctcg
     7861 ttcaccttgg gaatcacgac caaccacgcg cgtcacttcg tacgcgccgc cgcccgcagg
     7921 cgctccgcag tgcatcgatt cgcgcacgag cccaagagcg agctggcacc agccgcggat
     7981 gagcgcgtgg tacgcgaaca gctcgccgaa aggctcatgt gcgccatgga ccagctcagc
     8041 catgaccagc gcgttgcgtt cgtgctgtgt gaggtcgagg agcgctcctc cgaagaagcc
     8101 gccgctattc tggggatccc gagttcgacc atccgcgctc ggctcggagc cgcccgccaa
     8161 aaacttcagt ccttactcag cgaggagtgg ccatgaaagc cgacccattg gaggacgcgg
     8221 tttccgcact ccgcgagcgc tacgacggac agtcggacgc cgcatcgagg agtgaagatg
     8281 caatcatcgg tcggatgcgc cacaaaactc ggcagcgcca gcgtgcacgg tttgccatgc
     8341 cgttcgcggc gctgctcgcg gcttcgagcg cctgggcggc acgtgattcg atatctgaat
     8401 cggtcagaca ggtcggggcg tggctacgac atgagtcagc gcaatcgagt gggctgaatc
     8461 aacggggcgg gcaaccgcga tcccatgagc aaccgcggga ccaaggggaa ggggcggagc
     8521 gaccaaagcc ctcgcgtccg caaccagaag agctcgctcc gcacagcccc agctcggcgc
     8581 cggaaggcgc gccgaatacg ctgccgcgcg agccctccgc gcccacggca aagcctcgat
     8641 cgactccttc gccccggaat agcaccgctc ctacggctcg agcgcagaac gcgccatcgc
     8701 cgcagctcgg ggaacatccg gcaaagccgg acgccatgac gctctatcgg gaggcgcatc
     8761 gcctccactt cctcgataag aatccggagc aagccctctt ggcctgggac cgttacttag
     8821 cgcgcgggca aggcgacttt gccatcgagg cccgctacaa tcgggcactc tgcctatggc
     8881 gcctcggcag aatcgcggag gcgcagtccg agcttcgtcc gtttgcccgc ggagactatg
     8941 gcggataccg gcaagccgag gcggccgcgc tgctttccaa ctcggccgcc cctcacccat
     9001 gaatgaggcg gatcttgggg gggcgaaagc gcagacgtca gcttcctttg cgcccgcacg
     9061 aagcgaactc ggcagcacct ccgctttcgt ggcatgcatc gccacgccca tggcgaagtc
     9121 aaaaatcccg aaccccgatt cagggttggc tcccctaact cataatccct tcgccaagct
     9181 ggccgcgagt cagagcccaa cacctcccgg cacgcccgcc gccgttgccg ccgttgccgc
     9241 caaggctccg cctcaagagc gtgctcggga gcgcacgcgc gccatcaccg cgcggctaga
     9301 aacgcgaggc cgcagcggga aagtaatcac acgaatcgcg ggcttgccca caccggcgct
     9361 ggcgctcatc gcttcgcgct tacgccaagc gctcgggtgc ggaggaagcc tcgagggcga
     9421 ggacctgatt ctccagggct cgctggcgga gcgcgctacg gcttggctcc aaagcaccga
     9481 tttgagcgcg ctcacccgcg aagcaactca gcccaaaccc aagatcgtcg aaccggcggc
     9541 gcccccgtca tcgccggtgg cgctcgcttc gggaattcac cgtcgtgatg ttcgtccggg
     9601 caagcgcgtc gccgtcgtgc agaaagccga tcaagagagc ggggaactca ccttcggcat
     9661 cgtccgcgat attctaacga gctcgccgac tcacccacga gggatcaaag tgcgtctgca
     9721 aaccggcgag gtgggccgag tgaaagccgt ctacgacgcg taatccatgg cgccgctcgc
     9781 gctctcgcca ggcatcaatc cccaccacgc tgttgcggat aagggtcgac ctcgtcgagc
     9841 gcgcgcagct cgggcggtcc gagtgtctta tgcaggtcct cacccggctg gggcggggcc
     9901 ggcggggtgg ccgcagagcc ttcactcggc gcgataggcg ccgtcgcgac cgcgccaacc
     9961 acggaaccca aagcggcggt ccccacctgg cgaccctgcg ccgggttgcg catgccggcc
    10021 ttcgagcgtc tggccacgct gggccgcgcc gaaatcgaga cgttcgcggc actagacgaa
    10081 ggcacaacag ggctcaccac cggatcggcg agcgcgcgat cgatggcggg agacgcgagt
    10141 gagctaacgt caccaccgct cagttgctga cgccctcgag ccgtatctgc gtagggagtg
    10201 cccgaccaag cgactgcccc gatcaccgca aatgtcagcg cgaatacggc caccgtgcgg
    10261 atcgccgatc gtcgggcgct cagcttctcg ttcgaatacc atttggcgag gatgggtact
    10321 gaactcgagc tctccaaagg tgcgcccgaa tgtggcgccg ccacctccaa aaccgattcc
    10381 caggcgcccg gcgccgaatg agcttcgctt ctctgacttc ccgtgtcggc gagtaccttg
    10441 cgagccgtgt catgggtggt gccgagaatg cgcccaatcc gctccgccaa actaacgcct
    10501 tgggccgacg catacggcag cagcgctcgc gccagcatgg ccacgtccgg atagcgctgc
    10561 cgacgatcct tctggaggca gcgtgcgatc acctcctgta accccgtcgg cacgtcggcg
    10621 cggtagttcg acaacgcggg caccggctcg gtcgcgatgt tgatcgccac ctccccaaag
    10681 ctgtcccctc cgaacgggac gcgccccgtc agtagctcga acagcaccac gccgatgccc
    10741 cagatatcgc agcgcgcatc gacgtctttg gcattcttga cctgctcagg cgccatgtag
    10801 agaggcgagc cgagcactac gtccgttttc gtgaccgaca tgcgtagcag gccggggatc
    10861 cgcacgtcat cactcagctt cgaaaccccg aagtccatta ccttgataat cagttcatcg
    10921 tcactgcgct ggacgcagaa tagattggcc ggcttcaagt ctcggtgaac gatgcccaag
    10981 ctgtgcgcct gggcgaccgc aacgcacgcc tgcagcacga aatcgacggc ctgctccacg
    11041 ggaaacatgc cctcctgctc gagccaggtc tccaagtctt cgccctccag gtagtccatc
    11101 accatgtaag gcgcgcccga gtcgagccgc cctacgtcga acacgcgcac gacatgctcg
    11161 ctcttgattc tggcggccgc caaagcctct ctttcgaagc gcgccaccgc ctccgcattc
    11221 tgcagagctt cgggccacag gaacttgatc gccacccgat ctcccagtgc caagtgctgg
    11281 gcggccatca cataacccat cccgccgtgc ccaatgactc gttcgatgcg gtatttgtcc
    11341 gcgatcagtt gaccctcgat aacctcggtc tccatggctc tccagcgcga agcgttcgcg
    11401 ttcagccccg cagctccgat ccctgattaa ggagcaccat cgccgcgccc gcaagcaccg
    11461 aattttggct acgaatgtag ctaacccgcg ccacgttctc gtgcgagcgc gtaacgaacc
    11521 tatccccacg agaccaaccc gccctgctcg catacgggaa aagaccacgt ctcgaagcgc
    11581 gacgctgatc atcgcaccag cagtgacggc aatgtcgaaa agcgtgcccc gtctgttacg
    11641 ccgtccgaca cacactgcga gcattggacc gccccgtgtc tacgggcgcg aatacccacc
    11701 tagcccgtcg gcagctcagc cccagcgcaa gcggcccgcc cccacaccgc gcgagcggca
    11761 cgctgtgacg tgcaagacaa cgggcaagcg gcactcgacc gcaaacgatc gataaccccc
    11821 gtatctgaag agcttacaat ctagcgagct cgagcaggaa aggcttcacg cccaatcgcg
    11881 aagcccttcc cggacccgcg cgcccgcagg cgcaccgggt ggctcacgga agcagctgag
    11941 agaccagtga ccacttcgag gatttgccgg ccttcatcgc cagtacggta gtcagcgcga
    12001 gcacgcccgc ttcgaaggcc acattgagag cccccacgac attcacgatc ttctgcaggc
    12061 gcgcaacgcg tggtggagtt tcgggtgcgg catagtgccc gctctcgatg ggtacggggc
    12121 gggtcagctc gttatcgagc agggtacccg tgacgagtgc ccccacaccg gtcgtgaaag
    12181 ccccgagcac caacacgtcc ttcacgagcg tgagctcgcg ggacgtttgc cccacttcac
    12241 gaccggacaa ggcagctcgc ccgatcaacc aagtgccggc cgtggctgcg agcgcgatgg
    12301 cgttcacggt cttgtagcga tcccaagccg cgtggctcac cttgctgcgt tgttctttgg
    12361 aatcgatcac cttcaccgct ggattgagcg ccagctgtcc gaacaggttc cccccgaacc
    12421 cggcggccaa tccgagatcg tgtaaaaccc aagccgtcgt cgaaagcgtt gccatgagag
    12481 ctcctttgtc ttccgagcgt tttactcttc gagacctgtt tctcagaaac ggttcatcgg
    12541 ccaggctctg caagggcaat gccgcatgaa tcgctcggtt gcagcgtaga acgttgtcac
    12601 gtggcacttt gcttagactc acgaccgagg gttactctcg ctcggagtcc gcgcgctgag
    12661 ccaccgcgag tcgagacctg aaccacgtgt attgcccagc ttcgtccggg aagaaggtta
    12721 cttcgagcgg caatctttga ccctgtacgg tcacccagag gtaaaagttg tccaagctcg
    12781 taggctgaag cttcggctca tacgggagct gtgtcgagtc caacagcgga ccggacacca
    12841 gcaacgcccc acgctcggcg cgcagcatga cactcgagcc ctgagcgtct cgataggttc
    12901 cggcatattg ctccaagcgc gagggatcga cttcgcgacc cgctggcgga gcactgggcg
    12961 gtggtagtcc cccaaagctc tcgagtgcca gccgaatcga ttcgacgggg cgctctatat
    13021 ctcgattaga caagaccacg atcccgaagc cggtgctcgg gaacacggcg aaggtggagg
    13081 cgaaaccaga aatatcccct ccatgcccaa tgaacgggac tgggtagtac gtattgggtt
    13141 cggactggtg atccatgaag aatccctggc tgactccgac gcccaggccg tagtcgtcgg
    13201 ctaggccgag cgcggtcgcg tgaacatcgg agtagatcgt gtgagtcgaa atttgagagg
    13261 aaacgatctc gtggcgagaa agctcgctca gcacattcgg gtcgcctcgc atcaagaact
    13321 gcatcaggcg cgcccaatcg agcacgttcg aaaagcccag gcccgccggt cgcgcccaag
    13381 tgttctcgta gctgtccggg ctgatgtctt cgtaatcagt cacctcgctc gagacgctgt
    13441 tgatcccata tccgtggctg taatccccgt ctgcaagaac atcggaggcc aagaagaacg
    13501 atcgatccat atggagtgga gcaaacacgc gctcccgaat cgcctcacga tacgagacac
    13561 ccgtacttcg ttcgagggct cgaccggcaa tgtagaagtt gggattggaa tagttccaaa
    13621 acagccctgg tgggttgatg aagtcgacat tttcagccag ctcggccccc tccgcgaact
    13681 ccgcaagccc cgagtcttcg gctggccccg ggagctgtat atagtcgcgc agcccggatt
    13741 gctgcgacat cagcacacgc aacgtgagcg actccgcctc gggagaggct aacgagaggt
    13801 cgggaatggc ggccctgatc ggcgcgtcca agtcgagtaa gccatcttcg accgcggaca
    13861 tcgcgccgat cgcggtgatg accttaccca tcgatccggt gcgaaaaatg gtctttgcac
    13921 tgaccggctc tgtgctgttg atgcccttgg taccgaaccc tcgcgcgaag gacaattgtc
    13981 cgtgctccag aattgccact gccgcccctg ggatcccgag cgcgcgtctc tcgcgctcga
    14041 aacgctcggc gaaggcgcga aagcgagcgg gaatttcggt ttcttcaggt cggcgctgcg
    14101 gctctgcgtc actgctgcaa gcagacagcg tcaaccccag cgtcagcgcg atactcgccc
    14161 gcgatgcggt ttgaagactg gtgaacgtga gatgctccat tgccaactgt gcccgttcac
    14221 cgtgcggtcg ttcaaagacg tcgatcgcgc gaacgccggc gcgaggtcgc cggattgggc
    14281 ccagacgccc agtgtgccaa ttgtgtcgct cttcgggtct atgcgtggcg gacgcgcctc
    14341 gctaggatat ggcatgcgct ctaggtcctt cgccgcgcgc caagtgggta acgtggtcat
    14401 ttacgtccac acgtcggaac caccttcgga cgaggaatgg gaccgagtac taagcttcta
    14461 cgagggcgct gcaagcacgc tggaaccaaa gatcttggtc tacaccgagg gctcggcgcc
    14521 aaacgctgca cagcgcgcgc gcttgaatgg ggtgtttggc aagcgacggg tgttgctcgc
    14581 aatcttgacg tcgtcggctt tggcgcgagc agcgggaacc gcggtgcatt ggttccgacc
    14641 cgaggtgcgg gtgttcgggg ctcgggagtt tcccaaagcc gccatgcacc tcggactcac
    14701 tccagccgaa gcgaccgcca tcgagtccgc gctgaacgaa ctcaaaggcg aactccagct
    14761 cggggcaccc gcgccaaccc gtgaaaggtg agctcgggag ttaagaataa gaacctatcc
    14821 caggtctcag gatcgccgga gacggcggaa ggtataaaag gttctaaggc ttaggccgaa
    14881 tgcgcgactc aaacgacgga gtaacgcagc agcttgaggc actccaaagt ggcggccatg
    14941 tctcccgcct tcgcgccgtc cacgttggtg atgtgagggt tggcgccgag ctctagcagc
    15001 aaagcgacga tcggcgcttt gccgctagac gccgcgtaca tgagcgcgga ggcgccaacg
    15061 tcgttgcggt ggtccagatc ggcttgggca cgcgcgagcg cccgtacgat ctcgcccgag
    15121 ttggaaacgc acgccaacca tagcgccgtg ttgccgtcca tgttgcgtaa attcacgtcc
    15181 acacccagct cgaggagctc gttgacgagc ggccccgaac cgatcaagca cgcacgcatc
    15241 aacggggtca gcccgtaggc gccgggcgcg ttcaagtcgc gcgaatcgaa tccgtagtgg
    15301 accaagaact cgaggagggc ttcggtcatg tatcctcagc ttctacagct gcagtttcga
    15361 tgcgcgggtc gagctgagcc tgcccaacgc tgaagtggta aatcgaacgg tagggcgagt
    15421 tttgcaagcc gaggaactcg gccacgggtt catccagaaa gcagccaatg ccagtgcccc
    15481 ttacgccttc gagctctgcc tgcagataca gtgagtgtcc gatgcggcct gcttcgcggt
    15541 gcagatcgcg gtacgcggcg gggcagagct ccgccgctcg cgccacatca gccagcatcg
    15601 ccaccgcgaa gcagcagctg gccgcgattt cctgctgaca atggagcagg cggctgatcc
    15661 gagccagctc tctcgatgcg cagggggaaa cctggaacaa ctcgagttcc ggcgagctat
    15721 cgtgtactcg cctcaccggg aaatgggcct cgaaacacgg tagcaggccc gacggctttt
    15781 cggatacgtc gagccttggt ttgcccagct ctggttcacc cagctctggt tcacccggct
    15841 ctggttcacc cggctctggt tcacccagcc ctgcatcgct aggctccccg gcgcggcgca
    15901 ccaggtagat gccaggctct agggaatcca ctcgatgaac gaacaaaacg aggtggattt
    15961 gggtggcgac ctcaccatcc agaggcgtcc cgggcggacc ggaatcggaa gccgacggcg
    16021 aataagggca cgaagctgct ttcagcaggc gttcgaacgc ctggcgcgcc aacacgaaac
    16081 cagcattgaa gcgctgcgcg ctgcgccggc cctggactag ctgcgcagct gctagcggat
    16141 gatcgctcga cgcgactagc cgaggaggag cgactgggcc accccaggcc acgtcgcgac
    16201 cctgggggaa ctcctcgtct cggaagcgtg tcagtacggc ggtcgactcg acgatcggcc
    16261 aggtgtacat cgggtgagca tcgatgacgc ttgcgcgacc gtggaatgtg gcggtcgccg
    16321 cgcgctctcg cagctcgcgc gcgctcacgc tcg
//
LOCUS       DASTKU010001009         3056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_198252, whole genome shotgun sequence.
ACCESSION   DASTKU010001009 DASTKU010000000
VERSION     DASTKU010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198252"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..1603
                     /locus_tag="VFQ61_35800"
     CDS             407..1603
                     /locus_tag="VFQ61_35800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET9959929.1"
                     /translation="MAEERLQKVLARAGIASRRASEALITEGRVSVDGRIVRELGVRV
                     NARASRIEVDGKRLVAEPLVYIVLHKPRGVMCTLSDPEGRPTVAELLRDVGVRVVPIG
                     RLDFHTSGALLCTNDGDFAGKLLHPRGKVPKEYVAKVRGVVDERALERWRESILIDGR
                     ATQPADVHLMRVEGDKSWLSIVLKEGRNRQVRRLGEATGFEVLRLVRVAHAGITAEKV
                     LPGQWRHLTREELKALKQTYGVPHRIHTPPELDHMGGRLVRSSSAKARSQAAAPGARG
                     FRDNEDVPRPGRHEESGYRAASAPRDTKPSRFTSGTWGAEEGSRGRTERRPSGPARGE
                     SAGRGPSRRASGATGGRASGSPRREDAHARGRFSRQEGAGEGSRHAGPPRASRPERPT
                     RTRRTR"
     gene            complement(1714..2211)
                     /locus_tag="VFQ61_35805"
     CDS             complement(1714..2211)
                     /locus_tag="VFQ61_35805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959930.1"
                     /translation="MKPLSAGSEIDSYCTKCRMDLGHRVVAMVAGAPKRVVCLTCNSE
                     HNYRAPRTLKSNNTKDLGVRSRSDRASVPPKAPSASGGGSGGVRAASKAKAESERYEN
                     WASRTLGQAVDAFTRYSMERTFRAGELVLHPKFGEGYVDQVLEGGKVNVMFREGAKVL
                     AHRVS"
     gene            complement(2208..2696)
                     /locus_tag="VFQ61_35810"
     CDS             complement(2208..2696)
                     /locus_tag="VFQ61_35810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959931.1"
                     /translation="MPNERPPDGDDRDREQRRLERLIPEIVRRVVEAGYEKLTEGPEN
                     VRQIVSDLKLPKEALNMLLTQIDESKNGLYRAVAREVRDFLDNTNFAEELAKALTSLS
                     FEIKTEVRFIPNDARIKASPEVRTKMSVRRDRPSHPPSESHKPTTEAPPPPREKSEEN
                     SE"
BASE COUNT          532 a          950 c         1048 g          526 t
ORIGIN      
        1 atggcatacg cgacctaccg accgagcaac ccgcagccaa cggcgggggc gccaccccac
       61 caagcgaaac caagtcgaag tagcggagct cgaggaacgg agtgggctcg ttgagcaagg
      121 gtccgtaaaa ggagctccac ggacagagcc gcgctcggcc aaaggagcgc gagacgctcg
      181 ggcaccggag cgcggaggcg ctcgggcaac ggagcgcgga ggcgctcggc caacggagcg
      241 cagaggcgcc ctggtccacg tgtacaccat tcaaattcgc taggatcggg gccgttcgtt
      301 gcgcgccgcg agtgatgagc tatcgagctg cgcgaacggg tacctcgggt accgccgctc
      361 caaccacgat ctcgggccct gacccgagtt ttcacaaggc agagatatgg ccgaagaacg
      421 gctgcagaag gtgttagctc gcgcgggcat cgcgtcgcga cgcgcgtccg aggctttgat
      481 taccgagggc cgcgtcagcg tggacgggcg gatcgtgcgt gaactcggcg tgcgcgtgaa
      541 cgcgcgcgcg agtcgcatcg aagtcgacgg caaacggctg gtggcggagc cgctcgtcta
      601 tatagtgctg cacaagccgc gcggcgtgat gtgcaccttg agcgatccgg aggggcgccc
      661 cacggtggca gagctgctgc gcgatgtcgg cgtgcgggtg gtcccgatcg ggcgcctgga
      721 ctttcatacg agcggggccc tgctctgcac gaacgacggt gactttgccg gtaagctgct
      781 tcatccccgc ggcaaagtgc ccaaggagta cgtggccaag gtgcgcggcg tcgtggacga
      841 acgcgcgctc gagcgctggc gcgaaagcat tctgatcgat ggtcgcgcga cgcaacctgc
      901 ggacgtacac ctgatgcgcg tcgaggggga caagagctgg ttgtcgatcg tgctcaaaga
      961 aggacggaat cggcaggtgc gcaggttagg ggaggctact gggttcgagg tgctcaggct
     1021 ggtacgcgtg gcgcacgcag gaattacagc cgagaaggtg ctgcctgggc aatggcgtca
     1081 cctgacgcgc gaggagctca aagcgctcaa gcaaacctac ggtgtgccgc atcgaattca
     1141 cactccgccc gagctcgatc atatgggagg gcgtctcgtg cggagctcgt ccgcgaaagc
     1201 aagatcccag gcggccgcac ctggcgcgcg cggattcaga gacaacgaag acgtgccgcg
     1261 ccctggccgc cacgaagaat cggggtatcg cgccgcctct gcgccgcgcg atacgaaacc
     1321 ctcccggttt acttccggca cctggggagc cgaggaaggc agccgtgggc ggaccgagcg
     1381 cagaccgtcg ggccccgctc gtggcgagtc cgccggacgt ggaccgagtc gaagggcgag
     1441 cggcgccacc ggaggaagag cctcgggcag cccacgacgc gaggacgcgc acgctcgggg
     1501 acgattctct cgtcaagagg gcgccgggga gggctcgcga catgcgggac cgccgcgagc
     1561 ctcacggcct gagcggccaa cccgaacgcg acgtacgcgc tgaacaccct ggggactcaa
     1621 tcgagctccc gaggagccaa ggatcgaaac gcaaaaggcg cgaaacccgg ctggtttcgc
     1681 gcctgatcgg ctgctggtag cgtacggccg cgctcagctc acgcggtgcg cgagtacctt
     1741 ggctccctcg cggaacatga cgttgacctt gccaccttcg agcacctgat ccacgtagcc
     1801 ctctccgaac ttggggtgaa gcaccagctc accggctcgg aaggtgcgct ccatcgagta
     1861 gcgcgtgaaa gcgtcgacgg cttggccgag cgtgcgggac gcccaatttt cgtagcgctc
     1921 actctcggcc ttggctttgc ttgcggcccg aacccctccg gaaccgccgc cactggcgga
     1981 aggtgctttt ggcggtactg acgctcggtc cgatcgcgac cgcaccccga ggtctttggt
     2041 gttgttgctc ttcaaggtac gcggagcgcg gtagttgtgc tccgaattgc acgtgaggca
     2101 gaccacgcgc ttgggtgcac cagcgaccat cgcgaccacg cgatggccaa ggtccatgcg
     2161 gcacttggtg caatacgaat cgatttcgct tccagcagac aggggtttca ctcgctgttc
     2221 tcctcggact tctcgcgggg gggtggtggc gcttcggtag tgggtttgtg ggattcggat
     2281 gggggatggg aaggacggtc tcgccgcacg ctcatcttgg tgcgtacctc tggggaagcc
     2341 ttgatacgcg cgtcgttggg tatgaagcgg acctctgtct tgatttcgaa ggagagggaa
     2401 gtgagcgcct tggccagctc ctcagcgaag ttcgtgttat cgaggaagtc gcgtacctca
     2461 cgagccaccg cgcggtaaag cccgttcttg gattcatcga tctgggtgag aagcatgttc
     2521 agggcctcct tcgggagttt cagatcggag acgatctgac gaacgttctc gggaccctcg
     2581 gtgagcttct cgtaacccgc ttccactacg cgacgcacga tctccggaat cagacgttcg
     2641 agtctgcgct gttcgcgatc gcggtcatcg ccgtccggcg ggcgttcgtt cggcacgcaa
     2701 cacttttcgt accgtgcggg cgctgaggcg tcaaaccgaa cgacgccctc ccgcgcgcca
     2761 gagggatgtg ttcgaggccg gccgccgcac cgctgggtgt gctcgcgtcc tccggggcat
     2821 gccctgcgtc cgccccgggt tgaactgcca tgctgccgta tcgggctgta cccgcggctg
     2881 gtctagctct ctccacctct ctctacgtgt ctctacgcac cgtaccgggc acggctcacc
     2941 tggcgcacta gtgccgggac cttcctttcg gactttgagc gggtcgtgcc cctggttgga
     3001 ttggcgtcga gcgcgggctt ttcggccacg cgcaatctca ctcggctgct cccgcg
//
LOCUS       DASTKU010001010         7269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1539111, whole genome shotgun sequence.
ACCESSION   DASTKU010001010 DASTKU010000000
VERSION     DASTKU010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7269
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539111"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VFQ61_35815"
     CDS             complement(<1..961)
                     /locus_tag="VFQ61_35815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HET9959932.1"
                     /translation="MRIFDRDCSLEKEYMDGTHRSRRPADTLAAYSRWLAPLGITRLA
                     DVTGLDRVGIPVCLAIRPNSRSLSVSQGKGADKTAAKVSAMMESIELWHAENIENRLR
                     LETLYAMRKSCAIVAAEALPIRAGRVLRDDLPMLWMEGWDLIQQRATWVPFEYVSMNT
                     LCFSPEMTYCVSSNGLASGNHLLEAIEHALCELIERDATALKHLEPSEELVRCKVDPA
                     SVEDPLCRSFIERFRAADIEIAIWDITSDISIPTYEVMVIEPDRPRWRALGQFSGFGT
                     HPSPNVALSRALTEAAQGRLTIIAGSRDDNPYKVYAADAAEQRV"
     gene            complement(1386..2648)
                     /locus_tag="VFQ61_35820"
     CDS             complement(1386..2648)
                     /locus_tag="VFQ61_35820"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET9959933.1"
                     /translation="MSENPSSAIRYVAIGIIPLCLWSCESSAPTGGVAASGATAVPTL
                     GGSGGQTAAGGSASGAPSNSGSGGTTGGHVSTGAAGTGPLTSGGTMLAGASSISGSGG
                     APLITGGTSTQPSAGSGAGGRSAGGATGSSGVGASPTGGASTGGATAGVAGAGGAPDG
                     PCPPSATVKAGDNKKTVTTKDGERTFLVHAPPSYDGKKRVPVVFDFHGLSGNGAQQKS
                     LSRWDRLGDSQGFMTVYPDGLEQAWNAGLCCTDKYDDVAFVRAIIESLETDACIDTKR
                     IYASGCSNGGGMSYKLACEASDVIAAVAPVDFDCVAGTKCSQCSPTRPVSVVQFRGTK
                     DQLVAYEGDGAFSGAKNNFAKWGQLNMCTGSAAALTGHAGCESYPMCGSGSETVLCSV
                     ENGTHCGSYSSFMIPTVAWDVLQRHALP"
     gene            3078..3629
                     /locus_tag="VFQ61_35825"
     CDS             3078..3629
                     /locus_tag="VFQ61_35825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9959934.1"
                     /translation="MSEKSTTALRDEPPPRSDAERARLERMFATHHATIWRTLRRRGL
                     NPDAAADATQETFLIAAGRLSDIQPDSERAFLVGTALRVAHTLRRKTIRWQLDDEMDQ
                     RIATIGDERADLQLCDRALSKLNPELAEVFVLYEIEGLSSPEIAALLEIPLGSVASRL
                     RRAREQFRAAVSRMESARPGGRP"
     gene            3626..4414
                     /locus_tag="VFQ61_35830"
     CDS             3626..4414
                     /locus_tag="VFQ61_35830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9959935.1"
                     /translation="MSHGSRPTLDSESGLPDRLRSSGGTELERRLLNAASREQPSREL
                     SERMARAIGIALPPVAPVEPPGAARPEALTPNAGGLTKILAAARPLLPWLSATAVVIA
                     GAIVATRPKAPIATLPPTAAPAISSPSAALTASTVPAFASAIPQSRATEASPVTSSPP
                     AQVRSVPASSVADIQDQIALMDRARAALSAGASGRALELLRQYQTQYPRGSFRPEASV
                     LRIEALARLGRMSEARSLAERFAAESPNSPLSERVSRLLDAPRH"
     gene            complement(4441..5472)
                     /locus_tag="VFQ61_35835"
     CDS             complement(4441..5472)
                     /locus_tag="VFQ61_35835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959936.1"
                     /translation="MQAKPECTSRADLAARVESRLPHVRFVVEGTALVARARFTRLAS
                     GNVLGEVRLEGPGGAMATRRIVAQSCTQAAEAIALVISVTVDPNALLSTGSTNSGKVS
                     EGSSLPTRGERATSPSDAGEVTAGGLETKPVESPNLPAPAVNEQSANGSDQDSSGAST
                     LRGSDPVAFSGKPYFGTYVTAHAGLGVAPRPMMGVALYALAALESEGLFAPALMLGAA
                     HVARNGFVEQGGKANFELDTLSLDACPVRVRWELIGARACIWLLGGRLTATGSATSSP
                     TSVHRPFLAGGGSAVLTARLLPALELSARAAVGANFVRDSFEFSPFVFHTVSPVFASL
                     SLGLGIRSR"
     gene            5633..6394
                     /locus_tag="VFQ61_35840"
     CDS             5633..6394
                     /locus_tag="VFQ61_35840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9959937.1"
                     /translation="MTVVAGGACGGERVRLGDAIGGAHANTGGAAGVESGGNAGSGAS
                     TTTSGSAGEGGMTSCSHGLIQANQVLWIGDSWVTLPGEQHLAVRDFARAAGAIGMDED
                     YVVLATPAAGIEAISKQYEAQQSTTNPPKVVIMDGGTWDTLVDNGAEETVAAVVVSFE
                     RLLAKIAADGSVQHVIYYLMPELASIGGVAALRPPLMRACQQSTVPCHFIDLQPLWLN
                     HPEYTARDGIQASDAGAAVIAESIWGIMRENCIAQ"
     gene            complement(6430..>7269)
                     /locus_tag="VFQ61_35845"
     CDS             complement(6430..>7269)
                     /locus_tag="VFQ61_35845"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET9959938.1"
                     /translation="NRTFIVRVPPGYTGSTPVPVVLDFHPLGGTGSQQEGSSGWKAKC
                     DSVGCIAVFPDSSKSKASDNSWNAGYCCTNAQKNQVDDVGFARSIIKWLQTNACVDSK
                     RVYASGGSNGGGMTYRMACEAADVIAAVAPVDFRCVTGKDPLASAVSAANNTACSCTL
                     PRPISVVAWDEKADTSIVPYGGGQTPNLATDCAPNTSCVGIGFASAQVNAETWAKFNM
                     CTGAAAQDPGNPLCQTYSSCANNTTVSLCSTTSGGHLAVYSNSGAKFTDTAWNVFKTQ
                     SLP"
BASE COUNT         1345 a         2355 c         2301 g         1268 t
ORIGIN      
        1 gtacacgctg ttcggcggcg tccgcggcgt acaccttgta tgggttatca tcgcggctgc
       61 ccgcaatgat cgtgagtcgg ccctgggccg cctcggtgag cgcgcgggag agcgcgacgt
      121 tcgggctcgg gtgcgtgcca aatccggaaa attgccccag cgcgcgccaa cgcgggcgat
      181 ccggctcaat caccatcacc tcgtaggttg gaatggagat gtcggaggtg atatcccaaa
      241 tcgcaatttc gatgtccgcg gcccggaagc gctcgatgaa actgcggcat agcgggtctt
      301 ccacgctcgc cggatccacc ttgcaacgca cgagctcctc gctcggctcc aggtgcttga
      361 gcgccgtcgc gtcccgctcg atcaactcgc acaatgcatg ctcgatcgcc tcgaggaggt
      421 ggttgcccga cgcaagccca ttcgaactca cgcaatacgt catctccggg ctgaagcaca
      481 gcgtgttcat actcacgtac tcgaaaggca cccaggtcgc gcgctgttgg atcaggtccc
      541 agccctccat ccacaacatc ggcagatcgt cgcgcagcac tcgccccgcg cggatcggca
      601 acgcctccgc cgcgacgatc gcgcaggact tccgcatcgc gtacagcgtc tccagccgca
      661 gccgattctc gatgttttcc gcgtgccaga gctcgatcga ctccatcatc gccgagacct
      721 tggcggccgt cttgtccgca cccttgcctt gagagaccga gagcgaacgc gagttcggtc
      781 gaatcgctag acatacggga atgcccacgc gatccagccc cgtaacatcg gctagccgcg
      841 taatcccgag tggcgcgagc caacgagagt acgcagccaa tgtgtccgcg gggcggcggc
      901 tacggtgcgt gccatccatg tactcttttt ccagtgaaca atcgcgatcg aaaatccgca
      961 cgttttagtt ctcgctcacg tgagagtgca cctctgccga gcggcaaaga cgcagtctga
     1021 tccagattca accgagcctc gttcgccccg tttggacttg gggcagctca gtcgaggcga
     1081 cccagtcagg cggcctggtc agggcaacct ggtcaagggc aaacccatct ggttaaaccc
     1141 agcggggcaa ccagtgagca gagtaaacgc tgcgcacccc cgatcgcgca atagtaatga
     1201 gttgttcggg ttggcctcga ggtaaacgtg cgcgaactcc gcgcaaagcg agctgcaacg
     1261 cagtcactat gcgcactccc ctcgtcgcga caccgcgcct gccacccgcg ataacgccgg
     1321 atcacaccca ataacccgcg attgcagcga attacaggcg atcgaatcac acgctatatg
     1381 cgcgctcagg gcaatgcgtg ccgctgcaac acatcccaag ccaccgtcgg gatcatgaag
     1441 gagctatagc tgccgcaatg cgtgccgttt tcaacgctgc acagcaccgt ctccgaaccg
     1501 gaaccgcaca tcgggtagct ctcacaaccc gcatgacccg tgagcgcagc ggcgctgccc
     1561 gtgcacatat tgagttgtcc ccactttgcg aagttgttct tggctccgga aaaggcgccg
     1621 tcgccctcgt acgcgaccag ctggtccttg gtgccgcgga actgcactac ggaaacgggg
     1681 cgagtggggg agcactgcga acacttggtg cctgcgacac agtcgaagtc gacgggcgcg
     1741 acggccgcga tcacgtcgga agcttcacaa gctaacttgt aagacatgcc gccgccgttc
     1801 gagcatccgg aggcatagat gcgcttggtg tcgatgcagg cgtccgtctc gagcgattcg
     1861 atgatcgcgc gaacgaaggc cacgtcgtcg tatttgtcgg tgcagcagag gccggcgttc
     1921 caggcttgct cgaggccgtc ggggtaaacg gtcatgaaac cttggctgtc cccgaggcgg
     1981 tcccaacgag acagactctt ttgttgggcg ccgttcccgc tgagcccgtg aaagtcgaac
     2041 accaccggga cgcgcttctt gccgtcgtaa gaggggggag cgtgaaccag gaacgttcgt
     2101 tcgccgtcct tggtcgtcac ggtcttcttg ttgtctcccg cctttaccgt cgcggatggt
     2161 ggacagggtc catcgggggc accccccgcg cctgcaactc cggctgttgc tcctccggtg
     2221 cttgccccgc ccgtgggcga ggctccaaca ccggagcttc ccgtcgcgcc tccggcgctg
     2281 cgaccccccg cgccggaacc cgcgctcggt tgagtactag ttccacccgt gatcagcggg
     2341 gctccaccgc tgccggagat cgaggacgcg ccggccagca ttgtgccacc cgaggtgagg
     2401 ggcccggtgc ctgctgcgcc ggtgctcaca tgcccgcccg tagtgcctcc agatcctgaa
     2461 ttgctggggg caccgctcgc gctaccgccc gcggcggtct ggcctccgga gccacccaac
     2521 gttggaaccg cagtcgcacc gctggcggct acgccacccg tcggggcaga ggactcacaa
     2581 gaccacaggc agagtgggat gatcccaatt gcaacatatc gaattgcgga gctgggattc
     2641 tctgacattg ggggtctcct gggccacccg cgacgggtgc gttgagattc acgtcttcgt
     2701 ttggcgaccc tcgctactcc gcctaaccga catgctgtcg gctgacatgc gagcggcgac
     2761 gtgaggggcg gcagtgtcgt ggtgcgaatg cgggcgctta cacgaatcga tcaagaaaag
     2821 ttggagcgac cgcggcgtgc tgccgaacgg cagttttcgg ttcgcgccgc ccgttcttct
     2881 cacgccgaga cgcggggata acgcgttgcg tctcggcggt tccacgtgtg cgagaagagc
     2941 tttcgtgagc gcggagcgca tctaccgccc gaagtgagcc gttcgatgcg ggcatcagtt
     3001 tctttgatcg aaggagccct cgagccgcat tggcaagcgc ggcgcacggg cgaagcacgg
     3061 acgagtcaga agcgcggatg agcgagaaga gcacgacggc actgcgggac gagccaccac
     3121 cgcgatcaga cgcggagcgt gctcgtctgg aacggatgtt cgcgactcat cacgcgacaa
     3181 tctggagaac cctacgacgc cgcggtttga accccgacgc tgcagccgac gcgacccaag
     3241 agaccttcct gatcgcggcg ggacggctgt ccgacatcca accggatagc gagcgggcat
     3301 tcctggtggg gaccgccctg cgcgtggccc ataccctgcg ccgcaagacg atccgctggc
     3361 aactcgacga cgaaatggac cagcgcatcg cgactatcgg cgatgagcgg gccgatctac
     3421 aattgtgcga ccgagcgcta tccaagctaa atccggagct cgctgaggtg ttcgtccttt
     3481 acgaaataga aggcctgtcc tcgccagaga tcgcggcgtt gctcgagatt ccgctcggga
     3541 gcgtggcctc tcgcctgcga cgcgcgagag agcaattccg cgccgctgtt tcccggatgg
     3601 agagcgcgcg tcccgggggg cggccgtgag ccacggatct cgacccacgc tcgactccga
     3661 gtcgggtttg ccggatcgct tgcgttcgtc ggggggtacc gagctggagc ggcggctcct
     3721 gaacgcggcc tcgcgggagc agccatcgcg tgagctcagt gagcgaatgg cgcgtgcgat
     3781 tgggattgct ctgccgccgg tcgcgccggt cgagccccct ggagcggcgc ggccggaggc
     3841 gctcacaccg aatgctggtg ggttgaccaa gattcttgcg gcggcgcgcc ccctgctacc
     3901 ctggctatcg gctaccgcgg tggtcatcgc gggagcgatc gtggcaacgc gacccaaagc
     3961 tccaattgcc actctgccgc ccacggcggc tccagccatc tcttcgccga gcgctgcact
     4021 gacagcttcg accgtgcctg ctttcgccag cgcgattccg caatcgcggg cgacagaggc
     4081 ttcaccggtg acgagttccc cgcccgcaca agttcgcagc gtccccgcca gctcggtggc
     4141 ggacattcaa gatcagattg cgctcatgga ccgagctcga gccgcgcttt ccgcgggagc
     4201 gagcgggcgg gcattggagc tgctccggca gtaccaaacg caataccctc gcggcagttt
     4261 tcgacccgaa gcgagcgtgc tcagaataga ggcattggcg cggctcgggc gaatgtcaga
     4321 ggcgcgtagc ttggccgaac gcttcgcggc ggagtccccc aatagcccgc tctccgagcg
     4381 cgtctcgcgc ttactcgacg cgcctcgcca ttaggtacac gccttctcaa tccgcgggag
     4441 tcaacgcgat cgaatgccca accccaggct caaggaggcg aacacgggcg agaccgtgtg
     4501 aaagacgaag ggactgaatt cgaatgaatc ccgaacgaaa ttggcaccta ctgctgcccg
     4561 tgcggagagc tcgagcgcgg gcagtagtct ggccgtgagt acggcggacc ctccgccagc
     4621 caggaacggc cggtgaacgg aagtagggct actcgtggcg ctgccggtcg cagttaggcg
     4681 tccaccgagc aaccagatac aggcgcgcgc accgatgagc tcccatcgaa ctcgtacggg
     4741 gcaggcatct aaactgagtg tgtccaactc gaaattggct ttaccgcctt gctccacgaa
     4801 accgttccgc gcgacgtgcg cggcccctaa catcagcgcg ggagcgaaca gcccctcact
     4861 ctccaacgca gccaaagcat agagcgccac acccatcatg ggccgcggag ccacgcccag
     4921 cccggcatgc gccgtcacat acgtgccgaa atacggcttc cccgagaatg ccaccggatc
     4981 agagccgcgg agggtactag ctccgctcga gtcctgatcg gacccgttcg cgctctgttc
     5041 attgacagct ggtgccggca aattcggcga ctccacgggt ttcgtttcga gccctccggc
     5101 agtgacttct ccggcatcgc ttggtgaagt cgctcgctcg cctctagtcg gaagagagga
     5161 accttccgaa accttcccag agttggtcga ccccgtgctc agcagcgcat tgggatcaac
     5221 cgtgaccgaa atcacgagcg cgatggcctc cgctgcctgt gtgcacgatt gggcgacgat
     5281 gcgccgtgtg gccatcgcgc cgccgggccc ctcgagacgc acttcgccaa gtacgttgcc
     5341 ggaggcgagt cgtgtaaacc gcgctcgcgc cacgagtgca gtgccctcca cgacgaaccg
     5401 cacatggggc aaacggctct cgacccgtgc cgcgagatca gcgcgagagg tgcattcggg
     5461 cttggcttgc acctcgagct ggaccgtcga gagaggcgcc tccgcccgcg cggaccccgc
     5521 cgcgcaaaga agcggaagcg cgcgcagcag agccctgcaa actctcgcgc gacggtggta
     5581 tcgttccgcg gtgcatcgag cgacgcctct cggacgaatc attggcttgg cgatgactgt
     5641 ggtcgccggg ggcgcctgcg ggggcgaaag ggtgcggctc ggggatgcaa tcggcggcgc
     5701 gcacgctaac acaggcggcg cggccggtgt agagagtgga ggcaacgccg gatcgggcgc
     5761 gagcaccacg acctccggca gcgcgggaga gggcggtatg acctcatgca gccatgggct
     5821 cattcaggcc aaccaggtgc tgtggatcgg agactcctgg gtgaccctac ccggcgaaca
     5881 gcatttggcg gttcgagact ttgcgcgagc tgcgggagcg atcgggatgg atgaggacta
     5941 cgtcgtgttg gctacgccag ccgcgggcat agaggccatc tccaagcaat acgaggccca
     6001 gcagtcgact acgaacccac ccaaggtggt gatcatggac ggcggaactt gggacacgct
     6061 cgtcgataac ggcgccgaag aaacggtggc ggccgtggtc gtcagcttcg agcgcctgct
     6121 ggccaagatt gccgcagacg gctccgtgca gcacgtcatc tattacttga tgcccgaact
     6181 cgcgagcatc ggtggcgtgg ccgccctccg accgccgctg atgcgggcat gtcagcaaag
     6241 caccgtgcct tgccatttca tcgaccttca gccgctttgg ctcaaccacc ctgaatacac
     6301 ggcccgcgac ggtattcagg catccgacgc gggcgctgcg gtcatcgcgg agtcgatctg
     6361 gggcatcatg cgtgagaact gcatcgcgca gtagttctca cgcactatgt ccgaaacaac
     6421 gcctccaact caggggaggg actgagtttt gaatacgttc cacgccgtat cggtgaactt
     6481 ggcgccagag ttcgagtaca ccgccaggtg cccgccgctg gtggtcgagc aaaggctgac
     6541 cgtggtgttg ttggcgcacg acgagtaggt ttggcaaagt gggttgccgg gatcctgggc
     6601 cgcagctccg gtacacatat tgaatttcgc ccaggtctcg gcgttgacct gcgcgctggc
     6661 gaaacctatg cccacacagc tcgtgttcgg agcgcagtcc gtagcgagat tgggtgtctg
     6721 cccgccccca tagggaacaa tcgaggtgtc tgccttctca tcccaagcca cgaccgagat
     6781 cgggcgaggc agggtgcagg aacaagccgt gttgttcgcc gcgctgacgg cgctcgcaag
     6841 cgggtccttg ccggtgacgc agcggaaatc gacaggcgca accgcggcga tcacgtcggc
     6901 ggcctcgcac gccattcgat aggtcatgcc gccgccgttg gagccgccgc tcgcgtaaac
     6961 acgtttcgaa tccacgcagg cgttcgtctg cagccacttt atgatgctgc gcgcgaaacc
     7021 cacgtcatcg acttggttct tttgcgcgtt ggtgcagcag tagcccgcgt tccacgagtt
     7081 gtcgctggcc ttcgacttgg acgagtcggg gaacacggcg atacagccca cgctatcgca
     7141 cttcgctttc cacccggatg agccttcctg ttgagatccc gttcctccca acgggtgaaa
     7201 gtcgagaact accgggacgg gggtgctgcc ggtgtatccc ggcggaacac ggacgatgaa
     7261 cgtgcgatt
//
LOCUS       DASTKU010001011         5988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1315625, whole genome shotgun sequence.
ACCESSION   DASTKU010001011 DASTKU010000000
VERSION     DASTKU010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315625"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="VFQ61_35850"
     CDS             <1..1031
                     /locus_tag="VFQ61_35850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959939.1"
                     /translation="MTLNSNPRAPAIFRRVWPALGPVLLLMACGADRDPSGAALPSSE
                     PEPEPQTSPFKSLAVAARDSTMFTEPRAGVPLADGSVAFLATTTTEPERVGLFLQTAE
                     GPRLLYAGDQLVDARDLDSSLDEQALLAADPGLLVVNEEGTPQRGALLKFPLQGGEPE
                     LLAVGYAPVSVTVAEDGTYFSGTDPSSGEPGVFRLRAADPEAVYTGSPLVDPSGIALL
                     RDGSLLVADTRLFESTPINSEAGVVKIHGGQAEIFATGFATGYPAGIAVTSDESTLIV
                     SGEGPDRSDSVYLFSVADPSASPQVVTAEFSAVQNSSAGLKRARSGNTFVWASGAFQG
                     GTVYKIEG"
     gene            1034..2653
                     /locus_tag="VFQ61_35855"
     CDS             1034..2653
                     /locus_tag="VFQ61_35855"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HET9959940.1"
                     /translation="MKSLPLISFRLLRGALITTALLTTAFCGTALASSHREAPAIADD
                     PAADNTDLYAWLCEQPSGKALCVVANYNPLELPEGGPNYHKFSDDVLYQIHVTHNDAK
                     GRPSLLDYIVFDIRFETDPIQRVDVADPKAPPGGGKEFFAQLSQAFSQKVSVRARYSR
                     GTDYGNNSGFYFENEPSRVIFDRVPVAPPNIGQRTATFAYGNPNYDDFAKTFIKSNKA
                     GYRLFAGPRDDAFYVNLGQIFDLGAIVGLNSLNGQPFRSQKYDVNPNANGTFANTDPT
                     KALDGVAGFNVHSIALAIPLKELAPPGKEKEFDEAVNSGQPSNKTLFGVWASSMRRFI
                     TSGCFDNSRACRGGVRGDGPWRQVSRLGLPLINEAVIGIQDKDKFNRTTPLHDVNNFG
                     AYFLNPIIVRDVEAIGGYAQLGIDQVPDDFKFGRTDILQVINLDDIPTPGAHQVEIAN
                     GRTGDVLRVDVAVPSAFPNGRPLSGPAVKSGKEEDVTDIELSLLLSKLTVPISDYVNG
                     PGDGRKLLPEFPYLGSPFAGDTEGKGSPAQF"
     gene            complement(5717..>5988)
                     /locus_tag="VFQ61_35860"
     CDS             complement(5717..>5988)
                     /locus_tag="VFQ61_35860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959941.1"
                     /translation="SDWVTPRGARSPPRVKTPTSAGAHERSRSIKPPRSYPQSDEKAL
                     LVGARHIRRLLRPHGLPFFPPIFKKPSREDTVGPSRFAFQQENST"
BASE COUNT         1184 a         2034 c         1704 g         1066 t
ORIGIN      
        1 gtatgaccct gaattcgaac cctcgagcgc cggcgatatt ccggcgcgtc tggccagcgc
       61 ttggccccgt gctgctgctc atggcgtgcg gcgcagaccg cgacccgagc ggcgcagccc
      121 tgccctcgag tgaacccgag ccagaacccc aaaccagccc attcaaatca ctggccgtcg
      181 ccgcgcggga ttccaccatg ttcaccgaac ctcgcgctgg cgttcccttg gcggacggca
      241 gcgttgcctt cctcgccacg accacgaccg aacccgagcg cgtcggcctc ttcttacaaa
      301 ccgctgaagg gccccgtttg ctctacgccg gtgatcagtt ggtggacgcc cgcgatttgg
      361 attcatcact cgacgaacaa gccctgctcg cggcggatcc tgggctgctc gtggtgaacg
      421 aggagggcac tccgcaacgc ggcgctctgc tcaagtttcc actccaaggg ggcgaacccg
      481 aactcttggc cgtgggttac gcgcccgtca gcgtcaccgt cgcggaggat ggcacgtact
      541 tcagcggcac cgatccgagc agcggtgaac ccggcgtgtt tcgcttgcga gccgcagatc
      601 ccgaagcggt gtacacgggt tccccgctcg tcgacccgtc cggcatcgcc ctcctccgag
      661 acggcagcct actcgtggcg gatactcgtc tattcgagag cacacccatc aacagcgaag
      721 ctggcgtcgt gaaaatccat ggcggccagg ccgagatctt cgccaccgga tttgccactg
      781 gctaccccgc aggcatcgcc gtcacgagcg atgaatccac actgattgtc tcgggtgaag
      841 ggcccgatcg cagcgacagc gtgtacctct tcagcgtcgc cgatccgtcc gcttccccgc
      901 aggtcgtgac ggctgagttc tcggccgtcc aaaactcgtc tgctggcctc aaacgcgccc
      961 ggagcggcaa cacgttcgtc tgggcctcgg gcgcctttca aggcggcacc gtctacaaaa
     1021 tcgaaggttg accatgaaat ccttgcctct gatctcgttt cgactcctgc gcggtgccct
     1081 aatcaccacc gcactgctga caacggcatt ctgcggcacc gcactcgcct cgagccaccg
     1141 cgaagccccc gccatcgccg atgatccggc cgcggacaac accgatctct atgcttggct
     1201 ttgtgaacaa ccgagtggga aagcgctctg cgtagtggcc aattacaacc ccctcgagtt
     1261 acccgagggc ggccccaact accacaaatt ctccgacgac gtgctgtacc aaatccacgt
     1321 cacccacaac gacgccaaag gccgcccctc cttactcgat tacatcgtgt tcgatattcg
     1381 tttcgaaacc gatccgatcc agcgcgtcga cgtcgccgat cccaaggctc cccccggcgg
     1441 cggcaaagaa ttctttgccc aactcagcca ggccttctcg caaaaggtca gcgtacgagc
     1501 gcgctacagt cgcggcacgg actacggcaa caactccggc ttctacttcg aaaacgaacc
     1561 cagccgcgtg atcttcgacc gcgtacccgt cgccccgccc aacatcggtc agcgcaccgc
     1621 caccttcgcc tacggcaacc cgaattacga cgacttcgcc aagaccttca tcaaaagcaa
     1681 caaggccgga taccgcttgt tcgcgggccc ccgtgacgac gccttctatg tcaacctggg
     1741 acaaatcttc gacctcggcg ccatcgtcgg tctcaacagc ttgaacggac aacccttccg
     1801 ttcgcaaaag tacgacgtca accccaacgc caacggcacc ttcgccaaca ccgaccccac
     1861 caaagccctc gatggcgtcg ccggcttcaa cgtacacagc atcgccctcg ccatccccct
     1921 caaagaactg gccccgcccg gcaaagaaaa agagttcgac gaagccgtca attctggcca
     1981 accctccaac aaaaccctgt tcggcgtttg ggcctcctcc atgcgccgct tcatcaccag
     2041 cggctgcttc gacaactcgc gcgcatgccg cggcggcgta cgcggcgacg gcccttggcg
     2101 ccaagtctca cgcctcggct taccgctgat caacgaagcc gtgattggca tccaagacaa
     2161 agacaaattc aatcggacca cccccctcca cgacgtcaac aacttcggcg cctacttctt
     2221 gaaccccatc atcgtccgcg acgtcgaggc catcggcggc tatgcgcagc tcggcatcga
     2281 ccaagtccct gatgacttca aattcggccg caccgacatc ctccaagtca tcaacctcga
     2341 cgacatcccc acccccggag cccaccaagt cgagatcgcc aacggccgca ccggcgacgt
     2401 gctgcgcgtc gacgtcgccg tgccctccgc cttccccaac ggccgcccgc tgagcggacc
     2461 cgccgttaaa agcggcaaag aagaagacgt caccgacatc gaactctctc tcctcctctc
     2521 caaactcacc gtccccatct ccgactacgt caacggcccc ggcgacggcc gcaaactcct
     2581 ccccgaattc ccctacctag gctccccctt cgcgggcgac accgaaggca aaggcagccc
     2641 cgcgcagttc taatatcatc cccagcaatc acggaccctc gttcacccga gggcccgtga
     2701 ttgggttttg cgccactccg taaagagcgc gactcacggc gcggattcac tcgagcgcag
     2761 gcccaatcgc ccccagcccg tcccctcacc gcgccggcgc ccgcaataac gcacaatgcg
     2821 aaacaccgtc gggttgacgt caggggaccg cgttattgcg cgcggcgagc gcggtattcc
     2881 gagcgcagcg agcactttcg ctgcgccgcg gcgctcattc agctcacttc gcgcccgtta
     2941 catcgcttgc ccaaaccaga tctggagtgg tcccgaagtc gccaggcggc actagcgcca
     3001 cgtcccaaaa gtagtaacgt atttgacgct caggcttgaa ggaggcggcc ggagtaagtc
     3061 cacccgatca agcacaatct ggaccatcgc cgattcaaga tcgaagctcc tggttctgcg
     3121 gatccgctcg cgttcgagac gatcggggat cgactagtgt cccgagtccg caaaaagcaa
     3181 aagcgagcaa cccttgcgag gatttgatcc agctctcggg ccacttcgct gttcagcttc
     3241 cccgactcgc acagcaacac aatgcgtgcc ctgagcgcga gcgcctgccc cacgaatcac
     3301 ggactcagga cactagcgcc gcgccccaaa agtaataacg catttgacgc tcaggcttga
     3361 aggaggcggc cggagtaagt ccgcccgatc aagcacaatc tggaccatcg ccgattcaag
     3421 atcgaagctc ctggttctgc ggatccgctc gcgttcgaga cgatcgggga tcgactagcg
     3481 gcacttgaat tagcggagtt cattgcgtac cttccatccc acaaaactcc ttgtatgaat
     3541 gaactcggct gctatgctca cgacaaggaa ggggcgacgc atgggcgttt ggaaagtgcg
     3601 cgtttgcaag gatccgcggc agctaagtct cgatttccag cagcggaatt ggggcggcaa
     3661 acgaaaaggc gctggtcgga agggcggagg ccgaggagtc tcgcacgtga aacgcggcag
     3721 tcatcggcat tatcacccgg tgcacgtcac ggttcgcgtc gccgtgcgtt gtttgagagc
     3781 gcagcgcgtg ttcgtcgtca ttcgccgcgc gattcgcgcg gtgaacgcaa tgcgtgggga
     3841 gcgttttcgc atcgtacaat tttctgtaca gaccaatcat atccatctca ttgtcgaggc
     3901 taacgagaga gccgagctaa gtaacggcat gcgcgcgctg aatgcccgtg tcggcaagca
     3961 agtgaatcgt gttctcggta ggcgcggccg cttagtgtct gaacgacacc acgaacacgc
     4021 ccttgaatcc ccgcgcgctg tccgtcacgc cctctgctac gtgctcgcga acttcaaaaa
     4081 gcactccact catgaggcgc aacgtggaga accactcgac ccgtactctt cagcacagtt
     4141 cttcggaggt ttcgcggaaa gggtgaatta cgcatctcgc gcccaagact ccccggtcgc
     4201 tcccccgcgc acgtggctct tacgctcagg gtggcagagg cacggcctga tttcgatata
     4261 cgaggcgcca aggagttgag caaagtgaac tcacgttacc gcgaagtcac catcactaaa
     4321 cccgcgcggc cgggccaaca ctactgtcag agacttcaaa cagttcgctc gtcaagcccg
     4381 gctttggtcg aggctttcat tcggcggcgc agccgggagc acttgatggt gcagcgtggt
     4441 ctcgggacgc atgagatcag cgcgcccttg tcggcgatcg gcatgtcgtt cgtcgcggtc
     4501 aagcgacaca tcgccgcagg tttcgacggg ctctgcgacc gaccacgctc tcgctcgttg
     4561 gtcggtgcgg gctctagcgg agttcctggc gcgccaattg gccgcttcct gcgctcacat
     4621 cgagttcgct actgatccga acgcccccca cctcgatgcc aaagccgcca agatctgcaa
     4681 gctgtacatc tcgcctgctg caggcacgac ggtactttgc gtagacgaat acgagctcga
     4741 aggacccgca acatcttcgc cgctttcaac atccgcgacg gccgcgtgct gcttttggct
     4801 gagtaccatt ggcgtgcagc aatcggctga acaagcacag cgatctacgc agtgttgcac
     4861 tacgtagttt tggcgacgcc gcgtcgtcag ccgggctcgt caacacgccg aatcgcaaac
     4921 tcgcgcgtcg ccatgctgga cgcagcgtcc ggcacggcac tcgaaagcgc tctggcaagg
     4981 ctcacccggg ccgagcggcg ctcgacaggt accttgaagg caacgcaggt cggcgcgaca
     5041 aggtacgttg gggcaagctt cgccttccgc ggcggggggg cgcgcttcgc aacgcccgtg
     5101 gacgcagctc tgaccaggag cgcagttggc ggatgccagg caagggttgc caacggcggg
     5161 gttggcttcg caatggtggg tgcggagcga gcagtgctcg gtgcaaatcg gatgctgtgt
     5221 ctcgaattcg cgctgttgcg tgtacgcggc aagggcgtcg gtctgggttc cgcaagcggc
     5281 tccgattgga cgcggggggc tgcaacgtcc gggtcgaccg gaagttgcgc cttcacaata
     5341 taggccctcg cggcactcga gggctgattg gcaagcttgg ccctcgccca aacgaccccg
     5401 aatgatccga gtgcaagcgg ccggaggcga cggctgcccg gggttgaccc agccgcaacc
     5461 ctggaattgc gagccggccg ccccggcgca ggcccgaacg gcgttcggct cgagttcgat
     5521 ggcgcgccgt tcgaggctgc gggttagctc gctgacgcac tgttcataca agaagcgctt
     5581 gggttcaccg ccgcaacatt cagccctgcg tcggccgggt acgaggtgga ccgcgccgca
     5641 gaactgctca accagtgggt cggagcgccg atcagattcc accggggagg tttcccctga
     5701 ggattgaacc tgccattcaa gtggagttct cttgttgaaa ggcaaatcgg ctcgggccga
     5761 ccgtgtcttc gcgggatggc ttcttgaaga ttggggggaa gaacggcagg ccgtgaggac
     5821 gtaacaagcg ccggatgtgg cgagcaccaa ccagcagagc tttctcatcg ctctggggat
     5881 acgaccgagg cggcttgatg gatcggctgc gctcgtgggc gcctgcactc gtgggcgtct
     5941 tcacgcgggg cggcgaccgc gccccgcgtg gggtcaccca atcagagg
//
LOCUS       DASTKU010001012        10991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1539439, whole genome shotgun sequence.
ACCESSION   DASTKU010001012 DASTKU010000000
VERSION     DASTKU010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10991
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539439"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            459..2384
                     /locus_tag="VFQ61_35865"
     CDS             459..2384
                     /locus_tag="VFQ61_35865"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HET9959942.1"
                     /translation="MVNRGALCAVLCSCLAACGGSTNSGVTNGGAPTAGSGGGVAAGN
                     GGSMSVSGGAGAGQGGSMPNTASGGASTAGTSGTGGSGADLLVDDFEDGDGIPKVSGG
                     WYGYDDNPDGGKSTLTFTGAAAGAVAMNGVGYESQKSLEVSYTFDQGTLKYQPYVGLG
                     VSIGSSASPLDCSGYVGVSYTYQGGAHRLNVETTDVADYDHFGVALPASATWKTVTLP
                     FATLSQEGWGAKSAFDPKHVTNLAFAIRGTTGASAKLQVDNLKLVASIASGAPDMTIE
                     QPAPPVATPLDSIEIENPLQAKAMQYLTRGYNLTNWLEQERFSGFTYDESFVKKLAEA
                     GFRGLRLPVDFDLYVESANGEGEDLTLTVSDDLFEVLDAFDAWTKAHGLSLTIDYHQY
                     STLVQKSDPNSLATAVQLWGAVAKHFADNPREDLFLELLNEPELSFDADPTQDEWTAL
                     AERMIKAIRAEDTTHSLIFGDIGWYGIEKLASRAPLSDDNVIYAFHDYAPFIFTHQGA
                     TWANVGSTHDIPYPYSPERWSQYYADLGFNTAMESWILSDLRNYYRTGNRDALRNQII
                     MAKRWGIAHDVPVICNEFGAYDASSRLEDRARYYTDLIGIFDEIEIPWQHWFMVMDAQ
                     GQVIPEYRKAMGLDAQE"
     gene            2640..3251
                     /locus_tag="VFQ61_35870"
     CDS             2640..3251
                     /locus_tag="VFQ61_35870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959943.1"
                     /translation="MQFTYVFYLVPVLLGIAVLFGFSFYMRNLQLKRVQSMSPEEASR
                     RFNEFFSQYFELSPGETLVGIWSGVEFQGAQSAARQVAGNVANYVSAQAIGISTYIPM
                     VWIGQTSHGRVLVSREYSDFGARGNFKQLMSFQQGARALPAHVARQGQELGAPPKLPT
                     QPNVGLEFVQLRGASGEFYEAWMSPQGNRVGQTAYCSILQALG"
     gene            complement(3313..4137)
                     /locus_tag="VFQ61_35875"
     CDS             complement(3313..4137)
                     /locus_tag="VFQ61_35875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959944.1"
                     /translation="MNSTAPPTAPLSNAPAAENGAAKLRRRFVAWSIDLACTTAPLFP
                     LPFLQWESSFEWPSFYHGPPGGAWGGLERALSTFASVSFGVLIVLGLNTISVVRHRCT
                     LGMAYAGLSISGTRLWRWALSYFVAGPIVAGAAGYLALNLDAALGSGWLHWDLPDHAG
                     TWLLFVMLWLLVQVLPMLGRQRRTLFDHLARAHLKSSKLSDYGRYSRRRSSRGDWALM
                     GGCLGVVFVSVTDMSLYFPCFVGGMLGFSLVTAIQVHAYRARGGMLVHLARATARE"
     gene            complement(4179..4838)
                     /locus_tag="VFQ61_35880"
     CDS             complement(4179..4838)
                     /locus_tag="VFQ61_35880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959945.1"
                     /translation="MLAVLSLLNGCAGRTTEEPAPVQLSGGSTSTGGAGGSSGQSTLA
                     AGGVFIPPPGLGGSPGRCPSRSFNLDVHSAQTAPEEEGVWIETLQAPPPVYVPLFGAA
                     VNTDPETWDRSALPAGAGVFRLHGIDASCYPHGGYMYYGGSGDGPIEPYNFYETSQCE
                     SLPGCPSATVNDQDWGAWWYLTGNQTAADLIVCAQLFDKLLRASGIQLDLSSSGPTCR
                     N"
     gene            5081..5563
                     /locus_tag="VFQ61_35885"
     CDS             5081..5563
                     /locus_tag="VFQ61_35885"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET9959946.1"
                     /translation="MPARKPRSTGSQDVSSLETHLGYWLRFVSNHVSHAFGVKLSKRE
                     VTVAEWVLMRELYEHESVAPSKLAESLHMTRGAISKIVDRLAEKTLVARRQGEGDRRF
                     QELELTQAGRELVPVLAALADENDAEFFGHLGQRDRDFVQELLKEVVRRRQLSKIPVD
                     "
     gene            5665..6084
                     /locus_tag="VFQ61_35890"
     CDS             5665..6084
                     /locus_tag="VFQ61_35890"
                     /inference="COORDINATES: protein motif:HMM:NF018826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HET9959947.1"
                     /translation="MSSDKMDPSDRQALVEECTRASEEGRTTFPEVVAKLTQAGVERY
                     HADLLRAEKTYYWRDDSSLVVAATPVKHAFARDFSASEVEQAVRASQAGRIRYVEFCE
                     RVAAAGCVGYFVSLVGRRVVYYGRTGESHIELFPASR"
     gene            complement(6174..9593)
                     /locus_tag="VFQ61_35895"
     CDS             complement(6174..9593)
                     /locus_tag="VFQ61_35895"
                     /inference="COORDINATES: protein motif:HMM:NF012762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose binding domain-containing protein"
                     /protein_id="HET9959948.1"
                     /translation="MKHVNEASLGSARSANDSSTRGAVSTAESISSPPARRLGRLLGV
                     ATGAGVLAAACLFVGAGWIPSDYSPQPRAPISNTGAVRGLTVLVSFAAEGASTDHAIS
                     DDHAKKLYRMLNEKGYNADSNVGSLRDYYLDMSGGRFDLQSDVVRVTLPNPRSYYDTS
                     EEDNYDATGASSIQDVKISGSATRLLDDVLCRLVRCGGQTPRYDEVTATSVTGKTSDF
                     DFGVLSTSPLSFWPDHFIRSVSRDNRLLQPDSRRLIDIPKIAGIRHMGILYAGGTRQS
                     GGSKGLWPRTVPAVGTYLPNGNQSIRFGRFYIAGTIPPSGDPVAQTTPQPAFVALSAH
                     ESSHSMFGLPDLYDPGPDFPLRAGPSRPHTNGVGLHDIMGDAGSQQRPAVLGAPLRDR
                     LGWADVIDISDAAEGTRVTLKANTGQTARYCRQNSTARECFYIEARSKVAPRGPSLLP
                     MQTPDEGLAIWHAEDGQNILDSSVNNNPQGKPAQHYEVALLEADGRNELQNSGPYNGA
                     GDYFKAGLAERFDSTTSPSSNWWDGSASGLSIRRISAPGATMTFEIGRRPMSQVFVET
                     DANVQVNAGDRYLRVGQKRRITFTGRAGFRYDVSIRGSSSLDPKGLTGVQEIEITGSR
                     ADNHIKVVSYPADASAPVRSDLRRVRFVLTEGVEVVSFGDAGITRAAGGALIAPPSGR
                     MSFLPGRYENTSEPDLTYRTQAGQSAIKLVAKAQPGYVLKHLKTYAPGQDVASADGVS
                     HEYARQLAVDLVADQPNYTGNFLVSAYAEPIPGYLCRQGFVEEWSASQVYSNVGDLVR
                     RDDYIYASLVPHNILWSNVNPDYTPVATPNLTPESAPDHWARIASCKPYVHDCSAVQE
                     FKPSPAAPNLPQGAKVRLNGVVYEALTPNPPTTPGRNVSESEWTAASIYSEVDRSFPI
                     FDNRPFFYPRRGDWKLIGNCNTPELWKRAAIVPSTGVATITPVGVSSVQRHPSEPRYV
                     TNLNGAWSFNFTLDPGFALQDVYIDGRPLNLAPSARSASVPFSISEPRPSYIEIKTQC
                     SNSLCQSTITPSVSCTLGTPNVWGNGFQFNVTVTNLSGAQLTNWNVALPFAWTPTLWN
                     SANMKFQIEGNIATFSNGEAWNGKLAPNQSYTFSVSGPRSGPWVAPTCVGL"
     gene            9835..10836
                     /locus_tag="VFQ61_35900"
     CDS             9835..10836
                     /locus_tag="VFQ61_35900"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HET9959949.1"
                     /translation="MANKRWVLVQPTLEVAGRYGLSSQRLIEGLSEDREMREQAGLPL
                     EQVVKLWAEIATVARDPAFPFHAAEQLRLDRLGLPGFVILTAPTGRAAFDLAIRYASS
                     MVGRDCWQTQLRDGVMRIDWVQDGEPWLGNRVANEGAVAQTVVGLRQIYGAALRLDRV
                     TFRHSAPAAYRLHQQFFACPVEFGAEADALWFSERVLDTRPPLANPELAAYLQEQAEQ
                     SLRAQASDSVSRRLRLCIDRAFQDQEPELRIERYARGMGLSERTLRRKLKEEGTTFRA
                     VLSQAQRATAQHLIQQDNCSLTEIALRVGFSDSSAFSHAMRRWFGRSARHVRRNQSC"
BASE COUNT         2065 a         3218 c         3565 g         2143 t
ORIGIN      
        1 ttgcgctcga aggcgtcggg attcgcgagc ggcttacgag aatacttctc ggcgatgttg
       61 tcccagaatg cagcgtctac gggcatggtc cgcatacggt gcgaaggcag cgcgtgtgag
      121 tcaagtgtga gtttgcgaag taatgaccaa gggcgcgccg cgcctgccct ggcacactaa
      181 agcccctggg agagggccaa ttggaaggtc agcaatcgcg cgtgtccgtt ggggggtgag
      241 taatgtcgaa ttcgccgtgc cgttcgcggg cccgacgacc tcgcgtaatt tgcggtgcga
      301 ccaagggctg tcccgcaacg gggcggttcg cgccttcgac ggagctgggg aagctcatgc
      361 ggcgtacgtt ttcacgatct ttttcgtgaa tcgctgccat tactccggat gcgatgaccc
      421 aagccgctcg acccgttttg cgtacgaagt ctccaaccat ggtcaaccgc ggtgcgctat
      481 gcgccgtgct ctgcagctgc cttgctgcat gtgggggttc cacgaactcg ggcgtgacga
      541 acgggggagc gccgacggcg ggtagtgggg gtggggttgc ggcgggcaac ggcggttcga
      601 tgtcggtgtc cggcggcgct ggcgcgggac aaggggggag catgccgaat accgcgagcg
      661 ggggcgcatc tacggccgga acgtcgggta cgggtggcag cggcgctgac ctactggtgg
      721 acgacttcga agacggggac gggattccga aggtctccgg tggctggtat ggctatgacg
      781 acaaccccga cggaggaaag tcgactctga cgttcaccgg cgccgccgcg ggagcggtcg
      841 ccatgaatgg cgtcggctac gaatctcaga agtcactgga ggtgagctac acgttcgatc
      901 aggggactct gaagtaccag ccctacgtgg ggctcggggt ctcgatcggc agtagcgcga
      961 gccctctcga ttgctccggt tacgtggggg tctcttatac ctatcagggt ggtgcgcatc
     1021 gactgaacgt cgagaccacg gacgttgcgg attacgacca ctttggtgtc gcgctgcccg
     1081 cctccgcgac ctggaagacg gtaacgctgc ccttcgcgac gctcagtcag gaaggctggg
     1141 gtgccaaatc ggcgttcgat ccgaaacatg tcaccaatct cgctttcgcg attcgaggca
     1201 cgaccggcgc gagcgccaag ctacaagtag acaacttgaa gctggtggcc tcgatcgcga
     1261 gcggcgctcc ggatatgacc atcgagcagc ccgcgccgcc ggtggccacg ccactcgata
     1321 gcatcgagat cgagaatccg ctgcaagcca aggccatgca gtatctcacg cgaggttaca
     1381 atctgaccaa ctggctcgag caggagcgct tcagcgggtt cacctatgac gagagcttcg
     1441 tgaagaagct ggcggaagca gggtttcgtg ggttacggct gccggtggac ttcgatctgt
     1501 acgtggagag tgccaacggc gagggtgagg atctgacact caccgtctcg gacgatctgt
     1561 tcgaggtgtt ggacgccttc gacgcttgga cgaaggccca cggcttgtcc ctgaccatcg
     1621 actaccatca gtactcgacg ctagtgcaga agagcgatcc gaactcgttg gcgacggcgg
     1681 tgcagttgtg gggcgcggtg gccaagcact ttgccgataa cccgcgcgag gacctgtttc
     1741 tcgaattgct gaacgagccc gagctctcct tcgacgcaga tcccacgcaa gacgaatgga
     1801 cggccttggc cgaacgcatg ataaaggcga ttcgggccga ggacaccacg cacagcctga
     1861 ttttcgggga tattggctgg tacggtatcg agaagctcgc gtctcgtgcg ccactgagcg
     1921 acgacaacgt gatctacgca ttccacgact acgcgccgtt catcttcacc caccaggggg
     1981 caacctgggc caacgtgggc tccacgcacg atatccccta tccgtacagc cctgagcgtt
     2041 ggtctcagta ctacgctgat ctcggcttca acaccgcaat ggagtcctgg attctaagcg
     2101 atctgcgcaa ctactaccgc accggcaatc gggacgcgct ccgaaatcag atcatcatgg
     2161 ccaagcggtg gggcattgct cacgatgtgc cggtcatctg caatgagttc ggcgcctacg
     2221 acgcgagctc ccgcttggaa gatcgggctc gttactacac ggatctgatc gggatattcg
     2281 acgagatcga gatcccctgg cagcactggt tcatggttat ggatgctcaa ggccaagtca
     2341 tcccggagta ccggaaggcg atgggcctcg acgcgcaaga gtgaactcga gccgcggcgc
     2401 gggcagcccg gatctggggt cgcccgccac ccagtgcgtt tggaattggc cttgccaagc
     2461 tgcgccctcg tgctttactc ccgcgcggga tgtgatggta ctccgcgggt tctgctctac
     2521 gtcgcaacgc gactccactg ccgcacttgg gtatgggtcc agggtaggaa tggtgggaaa
     2581 gcattgcgag agcagccgag aagcggacgg agctcgtgct cgaggaaagg taactgaagg
     2641 tgcagtttac gtacgtattc tacctggtgc cggtgttgtt ggggatcgcg gtactattcg
     2701 gcttctcgtt ctacatgcgg aatctgcagc tcaagcgggt acagagcatg agcccggagg
     2761 aagcttcacg ccgcttcaat gagttcttct cgcagtattt cgaactcagt ccgggcgaga
     2821 cgctggttgg gatctggtct ggcgtggagt tccaaggagc acagagcgcg gcgcggcagg
     2881 tcgcgggcaa cgttgccaat tacgtctctg cacaagcgat aggcatctcg acgtacattc
     2941 cgatggtctg gattgggcag acttcgcacg gccgagtcct cgtctcgcgt gagtacagtg
     3001 atttcggggc tcgcggcaat ttcaagcagc tgatgtcgtt tcagcagggg gcgcgagcgt
     3061 tgcctgcgca cgtcgctcgc caaggacaag aattgggcgc gcctccgaag ctcccgacgc
     3121 aaccgaatgt ggggctcgaa ttcgtacagc ttcgcggggc aagcggcgag ttttacgaag
     3181 cgtggatgtc gccgcagggc aatcgagttg gccaaaccgc atactgttcg attcttcaag
     3241 ccctgggctg aatctctcgt tgcttgcttt tcccggtagc agataacagc tgcgggtagc
     3301 agccaagctt cgtcactcgc gagccgtcgc gcgcgccagg tgcaccagca tcccgccgcg
     3361 cgcgcgatag gcgtgaactt ggatggcggt gacgaggctg aatccgagca ttccgccgac
     3421 gaagcacgga aagtagaggg acatgtccgt gaccgagaca aacacgacac cgaggcatcc
     3481 gcccatgagc gcccagtctc cgcggctcga gcgtcgtcga ctatagcgcc cgtagtctga
     3541 aagcttgctg ctcttcaggt gcgcgcgagc cagatggtcg aacaaggttc tgcgttggcg
     3601 cccgagcatc ggtaacacct gcacgagcaa ccacagcatg acgaagagta accacgtgcc
     3661 cgcatgatcg ggtaaatccc agtgaagcca ccctgagccc aaggccgcgt ccaagttcag
     3721 ggcaaggtat ccggccgcac ccgcgacgat cggacccgcc acgaagtagc tcagggccca
     3781 acgccaaagg cgcgtgccgg agatggatag accggcgtag gccatgccga gagtgcaacg
     3841 gtgacgaacg actgagatcg tgttgagtcc gaggacaatc agcaccccga aggataccga
     3901 agcaaacgta gagagagccc gttcgaggcc tccccaggcc ccgccgggag gcccgtgata
     3961 aaagctcggc cactcgaatg aggattccca ttgcagaaac gggagaggga acaacggagc
     4021 ggtggtgcac gccagatcga tgctccacgc gacgaacctg cgtcgcagct tcgcggcgcc
     4081 attttctgcg gctggagcgt tggaaagggg agcggtgggc ggcgcggtcg agttcatgca
     4141 acgccctagt ggtttccgct cgacatgcct ggagcaggct aattccggca agttgggcca
     4201 gaagacgaga gatccagctg aatgcctgag gccctcagga gcttatcgaa tagttgagcg
     4261 caaacgatca gatccgccgc agtctggttg cccgtcagat accaccaagc accccagtcc
     4321 tggtcgttga cggtggcgct tgggcaacct ggtaacgatt cgcactggct ggtctcgtaa
     4381 aagttgtacg gctcaatggg accgtcccca gagccgccgt aatacatgta tccaccatgc
     4441 gggtaacagc tcgcgtcgat gccatgtaag cggaaaacgc ccgctcccgc cggtaatgca
     4501 gagcgatccc aagtctctgg gtcggtattc acggccgctc cgaaaagcgg aacatatacc
     4561 gggggcggcg cctgcagcgt ctcgatccag acgccctctt cctctggcgc ggtttgggcc
     4621 gagtggacgt cgagattgaa gctgcgggat gggcaacgtc ccggagaacc tcccagccca
     4681 ggaggcggga tgaacacgcc ccctgccgcc aacgtactct gaccgctgct tccgcccgcg
     4741 ccgccggtgg acgttgaacc gcccgatagc tggaccggtg cgggctcctc tgtcgttcga
     4801 ccggcgcaac cgttcagcaa cgagagcacc gccagcacgc tccaagctct cgctgccttt
     4861 gagcgtacac ggggccgatg ggcttttctc attggcgcat cttcctctct tcgtcctgta
     4921 gtcaggcacg gactccagag caaaactcat aacagaggcc gctcggcaat tctacccgct
     4981 ccgcaattta cccagtcctc ccgatttagt tgactaggaa accattcggc attagtttcc
     5041 tagtcaacta agtcgagtca accactgctt caccgttccc atgcctgccc gtaagcctag
     5101 aagcaccggt agccaagacg tcagctcgct ggagacgcac cttgggtact ggttgcgttt
     5161 cgtctcgaac cacgtctctc atgcgttcgg ggtcaaattg tccaagcgtg aggtgaccgt
     5221 ggccgagtgg gtgctgatgc gcgagctgta tgagcacgag tcggttgcgc ccagtaagct
     5281 agcggagtcg ctccacatga cgcgaggcgc gatttccaag atcgtggatc gtttggcaga
     5341 gaaaacactg gtggctcgga ggcaaggcga gggagaccgc cgatttcagg agctcgaact
     5401 gacgcaagcc gggcgcgaac tggtgccggt cttggcggca ttggcggatg agaacgacgc
     5461 ggagtttttt ggacatctcg ggcaacgaga ccgcgatttc gttcaggagc tattgaagga
     5521 agtcgttcgt cgtcggcagc tatcgaagat cccagtggat tgagcccagc taacctcgat
     5581 ctgcccgtct cctgcgctca gggttggctc tgcgtcaatt agactcggct cgaatcaccg
     5641 agcccagaga aaggatgatt tgtgatgtcg tctgataaaa tggatccgtc cgatagacag
     5701 gctcttgtcg aggaatgcac gcgggcttcg gaggaggggc gaacgacgtt cccggaggtc
     5761 gtcgcgaaac tcacgcaagc gggggtcgag agatatcatg cagacctgct gcgcgcggaa
     5821 aagacatact attggcgcga tgactcctca ctcgtcgtcg cggcgactcc tgtgaagcat
     5881 gccttcgcgc gagacttttc ggcgagcgag gtggagcagg ccgtgcgagc aagccaggcc
     5941 ggcagaattc gttacgtgga gttctgcgaa agagtcgcgg ccgccgggtg tgtcggatac
     6001 ttcgtctctt tggtgggccg gcgcgtggtt tactacggtc gtaccggcga gagccacatc
     6061 gagctttttc ccgcgagtcg ctgagtcgag ccaggattgt cctactcgag agctcagggt
     6121 acgcgcggcg ctttcgaggc gtaccctgag cacggtatgt gagacgtcga gtttcagagg
     6181 cctacacaag tcggggccac ccaagggcca gagcgcgggc cactgaccga gaaggtgtaa
     6241 ctctggttcg gtgcgagctt gccattccaa gcctcgccgt tcgagaacgt tgcgatgttg
     6301 ccttcgattt gaaacttcat attggcggag ttccagagcg tgggcgtcca cgcgaatggc
     6361 aaggctacgt tccagttcgt gagctgagcg ccgctaaggt tggtgacggt aacgttgaac
     6421 tggaagccgt taccccacac gttgggcgtt cccaacgtgc agctgacgct gggcgtgatg
     6481 gtgctttggc aaagcgagtt actgcactga gtcttgatct cgatatacga gggtcggggc
     6541 tcgctgatcg agaacggtac gctcgccgaa cgcgcgctgg gagccaaatt caatggccta
     6601 ccatcgatgt agacgtcctg gagcgcgaaa ccagggtcga gcgtgaagtt gaagctccag
     6661 gccccgttca agttcgtcac gtagcgtggt tcgctcgggt ggcgttgcac ggagctcacc
     6721 cccaccggag ttatggtggc gacgccggtg ctcggcacga tcgccgcccg cttccagagc
     6781 tctggtgtat tgcagttgcc aatcaacttc cagtcgccgc gacgcggata gaagaagggc
     6841 cggttgtcga agatcgggaa ggagcgatcc acctcggagt aaatcgaggc cgcggtccac
     6901 tcgctctcac tcacgttgcg ccctggcgtg gtgggcgggt tcggcgtgag tgcttcgtaa
     6961 accacgccat tgagacgcac cttggccccc tgcggcagat tgggagcggc ggggctcggt
     7021 ttgaactctt gcacggcgct gcagtcatgg acgtacggct tgcagctagc gatgcgcgcc
     7081 cagtggtccg gcgcgctctc cggggttagg ttgggggtcg cgaccggggt gtagtccggg
     7141 ttgacgttac tccacaggat gttgtggggc accagactcg catagatgta gtcgtccctc
     7201 cgaaccaggt cgcccacgtt gctatagacc tgagacgccg accactcttc cacgaatccc
     7261 tgtctgcaga ggtagcccgg aattggttcg gcgtaggcag agaccaggaa gttgccagta
     7321 tagttcggct ggtctgcgac caagtccacg gcgagctgcc gcgcgtattc gtggctcacc
     7381 ccatctgcgc tcgcgacgtc ttgccccggc gcgtaggtct tcaggtgctt gagcacgtag
     7441 cccggttgcg ccttggcgac caacttgatg gcagattgcc cggcttgggt ccgatacgtc
     7501 aggtcgggct cgctcgtgtt ctcgtatcgt ccgggcagga acgacatacg gcccgagggg
     7561 ggcgcgatca gagcgccgcc cgcggcgcgc gtgatgccag cgtctccaaa ggacacgacc
     7621 tccacgcctt ccgttagaac gaagcgaact ctgcgcaagt ccgaacgcac gggtgcgctc
     7681 gcgtccgcgg ggtacgagac gactttgatg tgattgtctg ctcgcgagcc cgtaatctcg
     7741 atctcttgaa cccccgtgag tcccttggga tcgagcgagc tgctcccgcg aatcgagacg
     7801 tcgtaacgga aaccggcgcg gcccgtgaaa gtgatgcgcc gtttctgccc gacgcggagg
     7861 taacgatcac cggcgttcac ctgcacgttg gcgtccgtct cgacgaacac ttgcgacatg
     7921 gggcggcgcc cgatctcgaa cgtcatggtg gcgccggggg ccgagatcct tcgaatcgag
     7981 aggccgctcg cgctgccgtc ccaccaattg gagctggggc tggtcgtact atcgaagcgt
     8041 tcggcgaggc ctgctttgaa gtagtcacct gcgccgttgt aggggccgct gttctggagc
     8101 tcgtttcgcc cgtctgcctc gagtagcgcg acttcgtagt gctgcgcggg ttttccttga
     8161 gggttgttgt tgacgctaga gtccaggatg ttctgcccat cttccgcgtg ccagatggca
     8221 agcccttcgt cgggggtttg catcggcagc aggctcggcc cgcgcggcgc caccttgctg
     8281 cgtgcctcga tatagaagca ctcacgcgcc gtggagttct ggcgacagta gcgcgccgtt
     8341 tgcccggtgt tggccttgag cgtgacgcgc gtgccctcgg ctgcgtccga aatgtcgatc
     8401 acgtcggccc agccgagccg atcgcggagc ggcgccccga gcaccgccgg ccgttgttgg
     8461 ctgcccgcat ctcccatgat gtcgtggagc cctacaccgt tcgtgtgggg ccgcgatggg
     8521 cctgcgcgca gcgggaaatc gggccctgga tcgtatagat cgggcaggcc gaacatgctg
     8581 tgcgagctct cgtgcgcaga gagagcgacg aaggctggtt gcggcgtggt ttgcgcgacg
     8641 ggatcccccg acgggggaat ggtacctgcg atatagaagc gcccgaagcg aatgctttga
     8701 ttgccattgg gcaggtaggt gcccaccgcg ggcaccgtgc gcggccataa gcccttcgag
     8761 ccgcccgact gtcgagtgcc gcctgcgtac agaatgccca tgtgtcgaat gcccgcgatt
     8821 ttggggatgt cgatcaatcg gcgagagtct ggttggagca ggcgattgtc tctcgagacg
     8881 ctgcggatga aatggtcggg ccagaaggag agcggtgagg tcgataaaac cccaaagtcg
     8941 aaatcgctcg ttttaccggt cacactggtg gcggtgacct cgtcatatct cggcgtttgg
     9001 cctccacaac ggaccagccg gcagagaacg tcatcgagca agcgtgtcgc cgagcccgag
     9061 atcttgacat cctggatgct cgacgcgccg gtcgcgtcgt agttgtcttc ctcgctggtg
     9121 tcatagtacg agcgtggatt cgggagcgtg acccgcacga cgtcgctttg aaggtcgaag
     9181 cggccgcccg acatatccag atagtaatcc cgtagagacc cgacgttgct gtcggcgttg
     9241 tatcccttct cgttcaacat tcggtacagc ttcttcgcgt gatcgtcgga gatcgcgtga
     9301 tcggtggacg cgccttcggc cgcgaagctc accagcaccg tcagcccgcg caccgcaccg
     9361 gtgttcgaga ttggcgcgcg cggctgcggg gaatagtcgc tcgggatcca gccggcccca
     9421 acgaacaagc aggcggcggc gagcacgccc gctcctgtcg ccacccctag caagcggccg
     9481 agccgccgag cgggcggcga cgaaatagat tctgccgtcg agacggctcc ccgagtagaa
     9541 gaatcgtttg cggatctcgc gctgccaagt gacgcttcat tcacatgctt catggacaac
     9601 ctctcgtggg cacgcgtctc gatgacgacg cggccagccc caaattctcg atgtttcagc
     9661 tcaccgagat tgagtaatca tcctaggggt tggtgatgac gccgacttgt tgcgcgcggc
     9721 caacgatttg gtccaaacgg ccaatcgtcg agtccgaccc gttggcggga aagaatcgac
     9781 ggggtcttcg acccattgcg aatcgaaacg acgacactat gcctccagtg gcgtatggcg
     9841 aataaacggt gggtgttggt gcaaccgacc ttggaggtcg cgggtcggta tgggttgagc
     9901 tcgcagcgtc tgatcgaagg gctttccgaa gaccgagaga tgcgcgagca ggcagggctc
     9961 cctctcgagc aagtcgtgaa gttgtgggca gagatcgcca cggtcgcgcg cgaccctgcg
    10021 ttcccgttcc atgcggcaga gcagctgcgc ttggatcgcc tcgggttgcc tgggttcgtg
    10081 atcctgacgg cgcccacggg ccgcgctgcc ttcgatttgg cgatccgcta tgcgtcctcg
    10141 atggttggcc gcgattgctg gcagactcag ctgcgcgacg gggtcatgcg gatagactgg
    10201 gtgcaagacg gagagccctg gctcggcaat cgcgtggcca acgaaggcgc ggtggctcaa
    10261 accgtggtgg gtttgcgcca gatttatggc gccgcattgc gcttggatcg ggtcacgttc
    10321 aggcattcgg cgccggcggc ttaccggctt caccagcaat tcttcgcgtg tcccgtggag
    10381 ttcggcgcgg aagcggacgc gctctggttt tcagaacgag ttttggatac tcgacccccg
    10441 ctggccaacc ctgaattggc ggcttacctg caagaacagg ccgagcagag tctacgcgcc
    10501 caagcaagcg attcagtttc gcgccggctg cggctctgca tcgatcgcgc gtttcaagac
    10561 caagagcccg agctccgaat cgaacgttat gcgcggggaa tgggcttgag cgaacgaacc
    10621 ttgcgccgga agctcaaaga agagggcacg acttttcgag ccgttctttc gcaggctcag
    10681 cgtgcgacgg cgcagcacct catccagcaa gacaactgct cgctcaccga gattgctctg
    10741 cgcgtgggct tctccgactc ctccgcgttc tcgcacgcca tgcgccgttg gttcggtcgc
    10801 tccgcgcggc acgtgcgccg gaatcagagt tgctgagccc ctcggacatc aactgttaac
    10861 tgcggcggtg gctctagatg ctggtagtgg cctcatacac ggtcacgccc ttgtgccagc
    10921 cttcggggcc ggcgccgctg cctttttcgt ggaagtgggg ctcgagacag aagatgtagc
    10981 ggcggccgcg a
//
LOCUS       DASTKU010001013         4324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1315731, whole genome shotgun sequence.
ACCESSION   DASTKU010001013 DASTKU010000000
VERSION     DASTKU010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315731"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VFQ61_35905"
     CDS             <1..1186
                     /locus_tag="VFQ61_35905"
                     /inference="COORDINATES: protein motif:HMM:NF018584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HET9959950.1"
                     /translation="TDYSRDPKPVQEALARLNEATEPMLAGMEVTDADLEELDRDFEQ
                     FYRYLCRKHLTKTPLKFSEPDRAAFYFYATKLAAVQHPNHFAWPLMSPLRGRDSRVFL
                     READNNHWARLALVTSALLKKDVALAERRVRELPPALAEIAIQWVRETAVGYAPYNHA
                     PSSELLLRALGAEITGPPSPQEDPLPRDQRWALSAGIYPSYGSDSRVALAVPLAPAVC
                     RAALKMWWNVDDSESAVSVLEWLLAEGHGQELRQELGRSDADELSKGQSRKMSIEEKR
                     KRAFLKSNGAALRKYSIKAWDLCRLVDVARSAHKAGFIDEATAWEYILAAARQLRRAY
                     PSWRAIGDDYLLGNRYFEKGGDPNVIQRACVEWLQNSPASPWNRISWDAFEPNYYGPK
                     LS"
     gene            complement(1201..3057)
                     /locus_tag="VFQ61_35910"
     CDS             complement(1201..3057)
                     /locus_tag="VFQ61_35910"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET9959951.1"
                     /translation="MKFRGNQWVFAGSVVFLLWACGGDSGSAEARGGSGPVEGGRRAT
                     GGAGGSSGAHRGGATSRGGEAAMGGAQELGGEAGACASGSCGGSDNSEGGAQPTGGQS
                     SAEGGSSGGTSVTGGRSSAGGTTDPGTAGRDGVSGASAGMGGQNGGGEIFVDPSSGSD
                     DAAGTREAPLQTIEKASALAVTRGSDAIRLLDGTYDATTERKFRAAPSSTCDADSGLV
                     LPSGIELTADHPGKAELIVTSGSGLCLSGARVSGLRLSRAALGGAVLSMDAGTVEIEE
                     TTFSNCGYPGISGFVSADALSACVVARGTAEVSLVPSSQEPVWRWGTVGTWIVALDSS
                     VVSVGSGIVEANSSAGSLFVAAGDSKLTISGVQATSNVNTIAMETQDRAEIAAQHGKV
                     TGFGVGLYTQSESTKATLEDWMFAETTTAVYLTGSKKEQTTVQLRAVEITGSQRAVVV
                     NNCEAALTIEDSTLRNNAIGVLFSGSGQLTLRDSRVEGGDRGVRAFAASGTYSVTLRN
                     TVIAQNKIVGLSLEGALLGTYDLGTVGSPGGNTLVGNASEAATPSNLVVIGTGNPTVQ
                     AVGNTWEKSLQGADANGQYVITGAGEKLEVQNGTGPNYVLGEGVKLRLAENP"
     gene            complement(3054..3419)
                     /locus_tag="VFQ61_35915"
     CDS             complement(3054..3419)
                     /locus_tag="VFQ61_35915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959952.1"
                     /translation="MGRRVQGALVEREVSGDRVPSLALDLHWALAPADGLPCALRIGS
                     GARGADLVPTRAEARKIEAEARKAEAEARAVAEARVRELEAELRRRSSPKSGSGWTLP
                     LSSGTLAELRSVTIGVDTG"
     gene            complement(4111..>4324)
                     /locus_tag="VFQ61_35920"
     CDS             complement(4111..>4324)
                     /locus_tag="VFQ61_35920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959953.1"
                     /translation="WPPVAKWWRAVALTRQSRNLGRAALGGAPRTGGAPRQSRNLGRA
                     ALGRAALPVSHETSDGRRSPRHAALA"
BASE COUNT          762 a         1467 c         1306 g          789 t
ORIGIN      
        1 gacggactac tcacgcgatc cgaagccggt gcaagaggcg ctcgctcgac tcaacgaagc
       61 cacggagccc atgctcgcgg gtatggaggt caccgatgcc gatctcgagg agctcgaccg
      121 agacttcgag cagttctatc gttacctctg caggaagcac ctcacgaaga cccccttgaa
      181 attttcggaa ccggatcgcg cagccttcta tttctacgcg acgaagctcg cggcagtgca
      241 gcatccgaat cactttgctt ggccgctgat gtcgccgctc cgggggcgcg actcacgggt
      301 attcctccgg gaggcggaca acaatcattg ggcccgcctg gcactcgtta cttcggcgtt
      361 gctcaaaaag gacgtagctc tggccgaacg gcgagttcgt gaacttcccc cggcactcgc
      421 cgagatagcg attcagtggg tacgcgagac agcggtcggc tacgcaccgt acaatcacgc
      481 gccgagctct gagctactcc tgcgggcact cggagccgaa ataacggggc cgccatcgcc
      541 ccaagaggat ccgctccctc gcgatcagcg ttgggcgctt tcggctggca tttacccttc
      601 ttatggctct gattcgcgcg tggctctcgc ggttccgctc gcgccagccg tgtgccgagc
      661 cgccctgaag atgtggtgga atgtcgatga cagcgagtcg gcagtctccg tgctcgaatg
      721 gctgttggct gagggtcacg ggcaagagct caggcaagag ctcggacgga gcgacgcaga
      781 cgagctctcc aaaggccaat cccgaaaaat gagcatcgag gagaagcgca agcgtgcctt
      841 tctgaaaagc aacggagccg cgctccggaa gtattcgatt aaggcgtggg acctgtgtcg
      901 cctcgtcgat gttgcgcgca gcgctcacaa ggccgggttc atcgacgagg cgaccgcgtg
      961 ggaatacatc cttgcggcgg cgcgccagct ccgtcgcgca tacccgagct ggcgtgcgat
     1021 cggcgacgac tacctgctcg ggaaccgata cttcgagaag ggtggtgatc ccaacgtgat
     1081 ccagcgcgcc tgcgtcgagt ggctccagaa ctccccggcc agcccatgga accggatttc
     1141 ctgggatgca ttcgagccga actactacgg tccgaagctc tcctgagacc acggtcgcaa
     1201 tcatgggttc tcggccaacc gcagcttgac gccttcgccc aatacatagt tgggtccggt
     1261 cccattctgg acctcgagtt tctcgcccgc tccggtgatg acgtattgac cattcgcgtc
     1321 cgcgccctgc agtgactttt cccaagtgtt gccgaccgct tgaacggtgg gattgccggt
     1381 gccgatcacc accagattgg acggtgtggc tgcctcgctg gcgttaccga cgagggtatt
     1441 gccacccggc gaaccaacgg ttccaaggtc gtaagttccg agcaaagcgc cttctagcga
     1501 gagtccgacg atcttgttct gcgctattac ggtgtttcgc agagtgacag aatacgttcc
     1561 cgaagcagcg aaggcgcgaa cccctctgtc ccctccctcc actcgggaat cacgcagggt
     1621 tagctgtccc gagcccgaga acaagacacc gatggcgttg ttacgcagcg tggagtcttc
     1681 gatggtcagt gcggcctcgc agttattgac gacgaccgcg cgctgagaac ctgtaatctc
     1741 caccgcgcgc agttgtacgg tggtttgttc cttcttcgaa cccgtcaagt agacggccgt
     1801 cgtagtctcg gcgaacatcc aatcttccaa ggtcgctttc gtactctcgg attgggtata
     1861 gaggccaacg ccgaagccag tcaccttgcc gtgttgcgcc gcaatctcag ctcgatcttg
     1921 cgtttccatg gcgatggtat tgacgttgct cgtcgcctgc acgcccgaaa tagtgagctt
     1981 ggagtcaccc gccgcgacga acaggctccc cgcggaggag ttcgcttcga cgattccgga
     2041 cccgacgctc acaaccgaag agtccagcgc gacgatccag gttccgacgg taccccagcg
     2101 ccaaaccggc tcttgagagg acgggacaag actcacctcc gcggtacccc tcgccacgac
     2161 gcaggcgcta agggcatccg ccgagacaaa tccggagatc cccgggtaac cgcagttgga
     2221 gaaagtcgtc tcttcgatct ctacggtccc cgcgtccatg gataagaccg cgccgccgag
     2281 ggcggcccga ctgagcctga ggccactaac gcgcgcaccg gacaaacaaa gaccgctccc
     2341 gctcgtgaca atcaactcgg ctttgcccgg gtggtccgcc gtgagctcga ttcccgaagg
     2401 taataccaga cccgagtccg catcacaggt ggatgagggt gcggctctga acttgcgctc
     2461 ggtggtcgca tcgtacgtgc cgtcgagtag ccgaattgca tcggagcccc gagtgacggc
     2521 gagtgcgctg gccttttcga tggtctggag aggcgcctcg cgcgtgccgg ccgcgtcatc
     2581 gctaccggat gagggatcta cgaaaatctc tcctccgccg ttctgcccgc ccatgccggc
     2641 gcttgcgccc gacacgccgt ctcgcccggc tgttcctggg tcagtagtcc ctcccgcgga
     2701 gctgcgtcca cccgtaacgc tcgttccgcc agagctgcct ccttccgcgg agctctggcc
     2761 gcccgtgggc tgcgcgccgc cctcgctgtt atcactcccc ccgcagcttc ccgacgcgca
     2821 ggctccggct tcaccgccga gttcctgagc cccgcccata gccgcttcgc caccacggct
     2881 cgtagctccg ccgcgatgcg ccccgctgct gcctcccgct ccgccggtcg cgcgccggcc
     2941 gccctcgacg gggcccgagc caccccgcgc ttcggctgag cccgagtcac ccccacaggc
     3001 ccatagcaag aacaccacac tgcccgcgaa cacccactga ttgcctcgaa acttcatcct
     3061 gtatcaaccc caatcgttac tgacctcagc tccgcgagtg tgccggaact caggggcaga
     3121 gtccatccgg agccggattt tggcgaggat cgacgccgca actccgcttc gagctctcgg
     3181 acccgcgctt cagcaacggc tcgagcctcg gcttcggctt tacgggcctc ggcttcgatt
     3241 ttacgagcct cggcgcgcgt ggggacgaga tcggcgcccc gggcgccgct gccgatgcgg
     3301 agcgcgcaag gcagcccatc tgcgggcgca agagcccagt gcagatcgag cgccaacgac
     3361 gggactcgat cgccggacac ttcgcgctcg acgagcgcgc cttggaccct tcggcccacg
     3421 atttgcctcg ggttcgaacc tgacgagttc gacgacccct gcgctcttgt agcgtcgcag
     3481 tttgtcggcc cagatgtctg ggttggaatc gtcgtcgctc acgacttcta ccgcaatctc
     3541 gggcgcacca cgctcccaag ttttccacga gcggatggga gcccctctcg ggctcagccg
     3601 cacatagacg tcaggggcaa cgcatcgacg aggatcgagc tcggcggaat tgtgacagaa
     3661 cacggagccg cggttcagcc gcgccgtcgt gagcgggagc acgacgtggt tgggaccgac
     3721 agatctcctg aagtgcgggc atattcattg tggccacctc catcgaagag cgcctgagct
     3781 ctgattggga atcgatcttt cgaacggcgt ttccgccact ccccagtctg ctcattgcca
     3841 atgttaccgg tccgtagttg cagagttctc tccagtgcag cgccggaatg atcgacgcta
     3901 tctcatcagc gctgcactgg aatcccgggg gccgtgtcag gccccccacg cggagcggag
     3961 cccgcgtttg gggggccctg ccgcgcgcag cggagcgagc acggcagggt gtccattgcg
     4021 ttcacgcttg aggggcaacc acgcgaacgg aacggcgtgt ccctgatctg cgcagcgcca
     4081 ccggttcgcg accgacgccc agcccgatgc tcacgccagc gccgcatgcc ttggggagcg
     4141 ccgcccgtcc gaggtttcgt gactgacggg gagcgccgcc cgtccgagcg ccgcccgtcc
     4201 gaggtttcgt gactgacgag gagcgccgcc cgtccgagga gcgccgccga gcgccgcccg
     4261 tccgaggttt cgtgactgac gcgtcagcgc caccgctcgc caccacttcg ccaccggcgg
     4321 ccag
//
LOCUS       DASTKU010001014        12633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1539519, whole genome shotgun sequence.
ACCESSION   DASTKU010001014 DASTKU010000000
VERSION     DASTKU010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12633
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539519"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3128
                     /locus_tag="VFQ61_35925"
     CDS             <1..3128
                     /locus_tag="VFQ61_35925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET9959954.1"
                     /translation="PLRLQVDEQSSVSALLKAVRAQVLAAFEHAVPFERILQATDVAR
                     RGTANPLVPIVMRHQNFPETSLGAGLPGDVQFRPYPDPNETDSEVLKLLAREHVPARC
                     EIELSYSGGAEELTVEVVYASDLYDREAVERLLGHHQRLLEGMMSDGSRRVLEVPLLR
                     DNDAQKLLRKVNRAPETRAPAQSFVQRFDAQVERAPESVACWDDQGAWTYRKLARHAN
                     QIAHALTARGIQPGDLVAVCLDRSGKLLATLLGIWKAGAAYVPLDPAYPSTYVQQILE
                     DARPSTVVCGAQHQALLELDDSRCLRLEQVWDADSKYPHRSPELGVGPEALAYVMYTS
                     GSTGKPKGVRVPHRQLDNWLSALEARLPFEAGEVVAQKTTFVFAVAVKELFAGLMNGC
                     PQVFIGNDTVRNTGAFVNALAEHRVTRLNIVPSHLASVMAHLQASGQRLPALKICVTA
                     GEPLPKSLVLTFRELFPSARLLNNYGCTELNDITYYDTASFDGRRDFVPIGTPIANTK
                     VYVLDRLGRLVPEGVPGELHVASVSVPEGYHGLESLTAERFLHNPFGTQPSDRLYNTG
                     DVVRYLSDGNLDFIGRWDFQVKVRGFRVDVRQVEKVLGDYEGIRARAVVGQGDRLIAF
                     YTTGPGKTVPLTELRGFLQDRLPAYMVPDAFVLLDAMPQLPNGKMNRRALLEWRGELQ
                     QSDAYEAPASATERTLADIWSFVLNVPHESIGRQTRFFDVGGHSLAAMRVLARIKDAF
                     RVELGVSQLFDEPRLDSIAAAIDRELALRPDSEREAQPSPSYRPAPKPHSGTGLLHGK
                     VALVTGASRGIGLATALLLAEHGAKVAINYRDSEAQARGVKEQIEAEGGIAEIFGADI
                     RNPSDVVRMVEAVHQRFECIDILIANAHINFRHRPFIEYEWADLEQKVNDELKAVFYP
                     SQAVAREMLKRKSGSIVAVSSTLSKRANEGFLAQSTAKAAVDAFVRALASELGPSGVR
                     VNTVAPGLTLTDAAMPMSPAVKEAIASKCPMRRNGLPEDMAGAVLFLASDLSRFMTGT
                     YLPVDGGFTML"
     gene            3174..3809
                     /locus_tag="VFQ61_35930"
     CDS             3174..3809
                     /locus_tag="VFQ61_35930"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET9959955.1"
                     /translation="MTAKLLVIDNYDSFTFNLVQMFRSYDLDISVFRADALSVEDVVS
                     QRPDYVLVSPGPKSPSAAGISTDLIRRCQGEFPIFGVCLGMQCMNEAFGGTTVHAPVP
                     MHGKTSAVDHDGTGLFQGVPQPFTVARYHSLAITDIHESLRVNARSADGVPMAIQHVR
                     HPLFGVQFHPESFLTQHGFTLVENFLRSGPLGGTRLQKTGLERAGLKGKLS"
     gene            3806..6115
                     /gene="pabB"
                     /locus_tag="VFQ61_35935"
     CDS             3806..6115
                     /gene="pabB"
                     /locus_tag="VFQ61_35935"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET9959956.1"
                     /translation="MTTVATDSSFSGIPQVIGVHAEPLDLQEPFVEVVRRFASLPGTV
                     ALMSGGQLDCARYHILGVDPWLSLSGRRTRTTLVVGERRLELEEDPFTSLRRVLQHCR
                     LPELDSSGAPAALPTPVRGGLLGYLTYDLKDCLERLPRTSIDDLELPLMQLVAPSILL
                     IRERASGQSTLLALRLQRDGAAGAEDAEFRERIARFKVALNAPATMPSPSAPQTPQAS
                     GKCVSVFSRAEYMSAVEAIRGYIVEGDVYQVNMSQRFQAPFSGDPFDCFASMYAANPA
                     PFFAYINAGDHQIVSTSPERFIELRDGTVETRPIKGTRPRGKTPSEDAALRTELQQST
                     KEDAELSMIVDLLRNDIGKVCRAGSVRVVEHKRLEAYQNVYHLVSIVKGELDAGMDAV
                     DLLRATFPGGSITGCPKIRAMEIIDELEPVRRHIYTGSIGYVGFDGTMDLSIAIRTAT
                     FTAGRVVFSVGGGIVFDSDPASEFEETLHKGRTLMNALDPASGGERSEATVWHNGAFK
                     PAAAASLPLDAEGLAYGFGFFETLRVQAGRPILLEAHLKRFELAWREFFQSTPPDVTW
                     ADVIAQLIHRNGLSQATAMVKLMAAAGKPGGVRPSHELFASAKPYSPRPVLKSQPGLR
                     LRGYPHSRHTHLASHKTLNYMFYKLAGDWAKRQGADEALILNADRSVSETNTAGVCCV
                     FGKTACFPVSEHALPSTMAGEVRRLLADWGFAVEDRRLTVEDVLSADHVFLTNSLMGA
                     VPAVSLDDKRLTYDSTLCTKLNHSLFDQG"
     gene            complement(6224..6556)
                     /locus_tag="VFQ61_35940"
     CDS             complement(6224..6556)
                     /locus_tag="VFQ61_35940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959957.1"
                     /translation="MFEVETRIIARTIGAAFVIMSYGCSVSPTENADTGVERFEMDLT
                     VAAPERAARDTTSVNSVDVEYASAARLYRICDYEPCRCLLDGVETSCALVESCLASGN
                     CVLVYRRN"
     gene            complement(6792..7628)
                     /locus_tag="VFQ61_35945"
     CDS             complement(6792..7628)
                     /locus_tag="VFQ61_35945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9959958.1"
                     /translation="MAKVAIVTGANQGLGFALVEGLCRALGEDASVYLTARDAARGNA
                     AREQLLAAGLEPIVHQLDVTSEASVQNFAELLGSKHGGVDIVISNAAARISKSTPQAQ
                     QVRTFIQTNNHGTHRVIRHFAPLLREGARFIVVASSFGSLRNLPPAHHAEFDVASRSL
                     EEIEVTLDQYVALVESGRHAQAHWPEWINIPSKVGQVAAMKIFARSFEREARARDILI
                     NAVCPGLIDTEASRPWFDDMSSAQTPAQAAVDVLWLATLAPGTSQPYGELVRHRQPVP
                     WS"
     gene            complement(7616..8026)
                     /locus_tag="VFQ61_35950"
     CDS             complement(7616..8026)
                     /locus_tag="VFQ61_35950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959959.1"
                     /translation="MAAQALVFEGAGAVVAEADGAPAREGTFDPAALVAGSLPFGGAG
                     VADASLLVGFAGRLGSGAGRHPATPTCQTVTSQTTFERPRGGANWVSKEERIAPKTLD
                     ATWEVNGPGVWFVESWSSGSTNGRLAQNRNAWRK"
     gene            8034..8918
                     /locus_tag="VFQ61_35955"
     CDS             8034..8918
                     /locus_tag="VFQ61_35955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959960.1"
                     /translation="MSANPTPFEKLFDGPVSSLSLGKAQRAVLLSGADVHLYDGKRLR
                     KLEPAVNPSADGLHANVFFGRDNEPRVLGYITPSPTAAGAQSPRTKYLRYRGGRLAEA
                     KDEIGSLASAQGVLYGVLGHDDPEVVCLAEKICIVKRLSGWKNIPAQSAAFEMVLAQG
                     QVFAWRETTLEQLSKTGFVALPSPPTRAPIRSVWLGPDQALWLVDGSTLHQRRAETWS
                     SLTAPIQNPTVVWGRSSNDVWLGGSDGAAHFDGQSWRCVDGVPGPITHIAGNESTLWI
                     AGPGGAYRSLASAAGTSR"
     gene            9452..10741
                     /locus_tag="VFQ61_35960"
     CDS             9452..10741
                     /locus_tag="VFQ61_35960"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9959961.1"
                     /translation="MQPFDASPTSEVSSPFSNLMSAPLSASSLAADTTSSARGSASTP
                     IDHHHMVVNDRPALRMVRGEGSYLWDEHGKRYLDFIQGWAVNCLGHSPPVIVDRVSRQ
                     MAQVLNVGPAFYNAPALELCERLAQASGLARVFLSNSGAEANEGAVKLCRKWGQKYRG
                     GAYQVITTVDGFHGRTLAMTCATGKAGWDEAFPPRVEGFVKASYGQLEPLRSLIGPQT
                     VAILVEPIQGEAGAVVPPDGYLKGLRELCDEHGLLLVLDEVQTGLGRTGPLFAFEHEG
                     VRPDILTLGKGLGGGLPISALLASERAACFEKGDHGSTFGGNAVMSSAGLAVLDELTR
                     EGARAERDASSAVLSRVLHELARELGATVRGRGHLFGMLFRRDVALTLQRVAFELGLI
                     INAPRPHILRFMPALNVSPEHCEEMSTLLREAARRVL"
     gene            complement(10754..>12633)
                     /locus_tag="VFQ61_35965"
     CDS             complement(10754..>12633)
                     /locus_tag="VFQ61_35965"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HET9959962.1"
                     /translation="QGPAPVPLPAAAAGAAVPVERYLVIDQFGYTPESAKVAVLVEPV
                     KGWRADHRYEPAPELELRNFNDGNVVFRGKIARWNEGRLDESAGDRGSWFDFSSVQVP
                     GTYYVYDPERKVRSHPFEIHKDVYRAVLKAATKMFYFNRANFAKVAPYACLGKKCWLQ
                     ARDYAGAGQDTEARSVRAREDAKTARDLSGGWWDAGDTNKYVTFSGEAVHQLLTAYSE
                     HPAAFRDDFGIPESGNGVPDLIDELVVELDWLKKMQPADLGGGALLKVGNVEYGDPVP
                     DQSQLRRFYYPEPCSSATIVLASELAHAALVLKAFPRFKAYSADLVQRAARAFTYYET
                     HPKSDACDDGTIKSGDADRSSTEQLQAAVTAAVYLFAATGQPKYAEFVDANYKSTRPF
                     QDETWSVYDPSQADALLYYVSLPKAAPLIKAEILARLGQLAGSVDLFGFKPELDLYRA
                     YMRSSSYHWGSNYARAATGNTNYHLVQHGLVEPSRQRDFRERALGILHSFHGVNAMQL
                     VYLTNMYALGGDACADEAYHTWFRDGSPQWDNARTSEFGPAPGYVTGGPNKDYCQGQD
                     PAQHACAKSPVRDQPPEKAYVDTNANWDPSNPYDKSWELTEPAIYYQAAYVRLVSKFV
                     E"
BASE COUNT         2296 a         4008 c         3999 g         2330 t
ORIGIN      
        1 tgccgctccg gctccaagtc gacgagcaga gcagcgtcag cgcgctgctc aaagcggtgc
       61 gcgcccaggt gctggccgcg ttcgagcacg cggtgccctt cgagcgcatc ttgcaggcta
      121 cggacgtggc tcgccgcggc actgccaatc ccttggtgcc tatcgtcatg cggcaccaaa
      181 atttcccgga gacatcgctg ggcgcggggc tgccggggga tgtgcagttc cgaccctatc
      241 ccgatccgaa cgagacggac tccgaagtct tgaagttgct cgcgagagag cacgtcccgg
      301 cacgctgcga gatcgagcta tcgtactctg ggggagcgga agagctcacg gtggaggtgg
      361 tgtacgcctc cgatctgtac gaccgtgagg cggtcgagcg tctgctcggt caccaccagc
      421 gcctgctgga aggcatgatg agcgacggga gccggcgtgt tctcgaggtg ccgctgctgc
      481 gcgacaatga tgctcagaag ctgctccgga aagtcaatcg cgccccggaa actcgagccc
      541 cggcccagtc cttcgtgcaa cgcttcgacg cgcaagtcga gcgggccccc gaatccgtcg
      601 cgtgctggga tgaccaaggt gcgtggactt accgaaagct cgcgcgccac gcgaatcaga
      661 tcgcgcacgc gctgacggca cgcgggatcc aaccgggtga tctggtggcg gtgtgtctgg
      721 atcggagtgg caagctcctc gccacgctgc tcggcatttg gaaggccggg gccgcgtacg
      781 tcccgctcga tccggcctac ccgagcacct acgtccagca aatcctcgag gacgcgcgcc
      841 cgagcaccgt ggtttgcggc gcccagcacc aggccttgct cgaattggac gactctcgct
      901 gcttgcggct cgaacaggtc tgggacgctg actcgaagta cccgcatcgt tcgcccgagc
      961 tgggcgtggg gcccgaagcg ctcgcctacg tgatgtatac gtcgggctcg acgggcaagc
     1021 ccaagggtgt gcgcgtcccg catcgtcagc tggacaattg gctgagcgca ctcgaagccc
     1081 ggctcccgtt cgaggccggg gaggtcgttg ctcaaaagac gacgttcgtg ttcgcggtgg
     1141 cggtcaaaga gctgttcgcg gggctcatga acggctgccc tcaggtgttc attggcaatg
     1201 acacggttcg gaatacaggc gcgttcgtca atgcgctcgc cgagcaccgg gtgactcgcc
     1261 tcaatatcgt gccttcgcac ctggcgagtg tcatggctca cctccaggcg agcgggcagc
     1321 gcctgccggc actcaagatc tgcgtcactg ccggcgagcc actcccgaag tcgctggtgc
     1381 tcacgttccg agagctgttc ccgagcgcgc ggctgctcaa caactatggc tgcaccgagc
     1441 tcaacgacat cacctactac gacaccgcga gcttcgatgg tcggcgagat ttcgttccga
     1501 tcggcacccc gatcgcgaat accaaggtgt acgtgctcga tcgcctaggc cggctggtgc
     1561 cagaaggcgt gccgggggag ttgcatgtgg cctccgtgag tgtgcccgag ggatatcatg
     1621 gcctcgagag cctgacggcc gagaggttct tgcacaatcc cttcggcacg cagccgagtg
     1681 atcgtctgta caatacgggt gacgtcgttc gatatctctc ggacggaaat ctggatttca
     1741 tcggtcgctg ggatttccag gtcaaggtac gaggctttcg cgtcgacgtg cgccaggtcg
     1801 agaaggtact cggcgactac gaaggaattc gagcccgcgc cgtggtaggc caaggcgacc
     1861 ggctcatcgc attctatacc accggccccg gaaagacggt gccgctgacg gagctgcgcg
     1921 ggtttctgca agaccgcctt cccgcttaca tggtgcccga cgccttcgtg ttgctcgacg
     1981 ccatgccgca gctgccgaat ggcaagatga accgccgcgc tctgctcgaa tggcggggag
     2041 agctacaaca gagcgatgcc tacgaggccc ccgcgagcgc aaccgagcgt actctggcgg
     2101 acatctggtc tttcgtcctg aacgtgcctc atgaaagcat tggtcgacaa acacgcttct
     2161 tcgatgtcgg cgggcactcg ctcgcggcga tgcgtgtctt ggcgaggatc aaagacgcct
     2221 tccgcgtgga gctcggggtg tcacagctgt tcgacgaacc gaggctcgac tcgattgcgg
     2281 ccgctatcga ccgcgaactt gcgctccggc ccgacagcga gcgcgaggcc caaccctccc
     2341 ccagctatcg acccgcgccg aagccgcaca gcggcacggg gctgctccac ggcaaggtgg
     2401 cactcgtcac cggcgccagc cgcggaatcg gattggctac cgcgctgctc ctggcggagc
     2461 acggcgcgaa ggtggcaatc aactaccgag atagtgaagc gcaggcgcgg ggggtcaagg
     2521 agcaaatcga ggccgaggga ggaatcgccg agatctttgg cgcagacatc cggaacccga
     2581 gcgacgtcgt ccgaatggtg gaggcggtgc atcagcggtt cgagtgcatc gacattctga
     2641 tcgcgaacgc ccacatcaac tttcgccatc gcccattcat cgaatacgag tgggcggatc
     2701 tcgagcagaa ggtcaacgac gagctcaaag cggtattcta tccttcccag gcggtcgcgc
     2761 gggagatgct caagcgcaaa agcggcagta tcgttgccgt ctcgagcacc ctctccaaac
     2821 gcgcgaacga ggggttcctc gcgcaaagca cggccaaggc tgccgtcgac gccttcgtgc
     2881 gtgccctggc gagcgagcta ggcccatccg gcgttcgggt caatacggtg gcgccggggc
     2941 tcacgttgac cgacgccgcc atgccgatgt cgccggccgt gaaggaggca atcgcttcca
     3001 agtgcccgat gcgccgcaat ggcttgcccg aggatatggc cggcgcggtg ctcttcttgg
     3061 ccagtgacct ttcgcgcttc atgaccggca cgtacctgcc cgtggatggc gggttcacga
     3121 tgttgtagcg gccgcgctgg gcatcattac tgaagaaaac tcgaggccag agcatgactg
     3181 ccaagcttct cgtcatcgac aactatgact ctttcacgtt caacctcgtc cagatgtttc
     3241 ggagttacga tctggatatc tcggtctttc gcgccgacgc gctctcggta gaagacgtag
     3301 tgagtcagcg gcccgattac gtgctcgtga gccccggtcc gaagagcccg agcgccgctg
     3361 ggatctcgac cgatctcatt cgtcgctgcc agggcgagtt ccccatcttc ggcgtgtgtc
     3421 tcggcatgca gtgcatgaac gaagccttcg gcggcaccac cgtacacgct cccgttccga
     3481 tgcacggcaa gacgagcgcc gtagatcacg atgggacggg tctcttccaa ggcgttcccc
     3541 aacccttcac ggtcgcgcgc taccactcac tcgcgattac ggacatacac gagagtctga
     3601 gggtcaacgc gcgctctgcg gacggggtac ccatggcaat tcagcacgtt cggcacccgc
     3661 tgttcggggt ccagtttcac cccgagagct ttctgaccca acacggcttt accctggtcg
     3721 agaacttcct gcgttcaggg ccactcggcg gaacgagact gcaaaaaaca gggctggagc
     3781 gagcgggatt gaaaggaaag ctatcatgac gacggtggca accgactcta gcttctcggg
     3841 aattccccaa gtgatagggg tgcacgcgga gcccctcgat ttgcaggagc cgttcgtgga
     3901 agtggttcgc cgcttcgcct cgctcccggg tacggtggcg ctgatgagtg gggggcagct
     3961 cgattgcgcg cgctaccaca tcctcggtgt cgatccgtgg ctttcgttga gtggacggcg
     4021 cacgcgcacc acgctggtcg tcggagagcg gcgcctcgag ctcgaggaag atccgttcac
     4081 ctcgttgcgc cgagtgttgc agcactgccg cctgccagag ctggattcat ccggggcgcc
     4141 ggcggctcta ccgacacccg tgcgcggtgg attgctcgga tatctgacct acgatctgaa
     4201 agactgccta gagcgcttac cccgaacgag catcgatgat ctcgagttgc cgctgatgca
     4261 gctcgtggcc ccttctattc tgctgattcg agagcgcgcc tccggtcaga gcacgctact
     4321 ggcgctacgc ttgcagcgcg atggtgcggc aggtgcggag gatgcggagt ttcgagagcg
     4381 catcgcgcgc ttcaaagtcg cactgaacgc gcctgcaacc atgccttcgc cctctgcgcc
     4441 tcagacgccg caagcgagcg ggaaatgtgt gtccgtgttc tcgcgggccg aatacatgtc
     4501 ggcagtcgag gccattcgcg gatacatcgt cgagggcgac gtgtaccaag tcaacatgtc
     4561 ccagcgcttc caggcgccgt tctctggcga cccgttcgac tgcttcgcga gcatgtacgc
     4621 agctaatccg gctccgtttt tcgcgtacat caacgctggt gatcatcaaa tcgtctcgac
     4681 ctctcccgag cgcttcatcg agctcagaga tggcaccgta gagacgcgcc caatcaaagg
     4741 cacccgccct cgcggcaaga cgccgagcga ggacgcggcc ttgagaacgg agctgcagca
     4801 gagcaccaaa gaagacgccg agctgtcgat gatcgtcgat ctgctgcgca acgatatcgg
     4861 gaaggtttgc cgggccggct ccgtgcgggt ggtggaacac aagcgactcg aagcctatca
     4921 aaacgtgtat cacctggtct cgatcgtgaa aggcgagctc gacgccggca tggacgccgt
     4981 cgacctgctc cgagccacct tcccgggcgg gtcgatcacg ggctgcccga aaatccgcgc
     5041 catggaaatc atcgacgagc tcgagccggt gcgcaggcac atctacacgg gcagcattgg
     5101 atacgtgggg ttcgacggga cgatggatct atccatcgcg attcggaccg cgacgttcac
     5161 ggccgggagg gtcgtcttct cggtcggcgg gggcatcgta ttcgactccg atcccgctag
     5221 cgagttcgag gaaacgctac acaaggggcg cactctgatg aacgccctcg accccgcgag
     5281 cggcggtgaa cgaagcgagg ccacggtctg gcacaatggc gcgttcaagc cggccgcggc
     5341 ggcgtcgctg ccgctcgacg cggaaggact ggcgtacggc ttcggatttt tcgaaacgct
     5401 gcgcgtccaa gcgggccgcc cgatcttgct cgaggctcac ttgaagcgct tcgagctggc
     5461 atggcgggag ttcttccagt ccacaccgcc ggacgtcacg tgggcggatg tgattgccca
     5521 gctgattcac agaaatggtc tttcgcaggc cacggccatg gtcaagttga tggcggcggc
     5581 aggcaagccc ggcggagtac gcccttcgca cgagctcttc gcgagtgcca agccctactc
     5641 gccgcgcccg gtgctgaaat cgcagccagg gctgcgactt cgcggatatc cgcatagccg
     5701 gcatacgcac ctcgcgagtc acaaaacact gaattacatg ttctacaagt tggctggcga
     5761 ttgggccaaa cggcaaggcg cagacgaggc cttgatcttg aatgcggatc gctcggtatc
     5821 cgagacgaac accgcgggcg tgtgctgcgt attcggcaag acggcgtgct tccccgtctc
     5881 ggagcatgcg ttacccagca ccatggccgg cgaagttcgg cgactgctag cggactgggg
     5941 cttcgcggtg gaggatcggc gcctgacggt ggaggatgtt ctctccgctg atcatgtgtt
     6001 cttgacgaac tcgttgatgg gcgcggttcc ggcggtaagc ctggacgaca agaggctcac
     6061 ttacgactcg accttgtgca cgaaactcaa ccactccctc ttcgaccagg gttgatggca
     6121 aaaaggtcga ggagcaagaa attgaggggc accgaagact gagggcacga gccattcgtc
     6181 gccgtcgcca aaccccgatc ctgttcccac gcacgcgcta gtactagttg cgccggtaca
     6241 cgagtacaca gttgccggag gccaaacaag actccacgag cgcacaagag gtctcaactc
     6301 cgtccagcaa acacctacaa ggttcataat cgcagattcg gtataaccga gccgcactgg
     6361 catattctac gtcaacggag ttgacgctgg tcgtgtcgcg agcggcgcgc tccggcgcgg
     6421 ccaccgtgag gtccatctcg aagcgttcga cgccagtgtc ggcgttctcg gtcggcgaga
     6481 ccgagcagcc atagctcatg atcacgaacg cagcaccgat ggtgcgagca atgatgcgag
     6541 tttctacctc gaacatggtg ttctccttgg cggtttctcc cgaatgggat gattcgattc
     6601 gattgccccc cggcctgcgt cgcccgccag gtatacggtc gctccatggc tggagttggc
     6661 aatggtcatt gcgcagtctc gtcgatgcgc gccgacgctc tggcgcgacc gctgtgctgc
     6721 tgtgcggctc catgaacctc gacgagtgag gcgcaccgcg taaacatcag tttcggtagc
     6781 cagagattgc tttacgacca aggcacgggc tggcgatgcc tgaccaactc accgtacggt
     6841 tgcgaggtgc ccggggcgag cgtcgcaagc cacagtacgt caaccgcggc ttgggccggg
     6901 gtctgggcag acgacatatc gtcgaaccat ggtcgtgaag cttcggtgtc gattaggccg
     6961 gggcagaccg cgttgatcag aatgtcccgt gcgcgggcct cgcgctcgaa cgaccgcgca
     7021 aagatcttca tggctgctac ctgccccact ttggatggaa tgttgatcca ctcgggccaa
     7081 tgcgcctgtg cgtgacgtcc agattccacg agcgcgacgt actggtccag ggtgacttcg
     7141 atctcctcga gcgacctgga ggcgacgtcg aattcagcgt ggtgtgccgg cggcaagttg
     7201 cggagcgagc caaaactact cgctacgacg atgaacctcg cgccctcgcg aagtagtggc
     7261 gcgaagtgcc gaatgacccg gtgcgtgccg tgattgttag tctggatgaa agtccgcact
     7321 tgctgcgctt ggggagtcga cttgctgatg cgtgcggcgg cgttcgaaat cacgatgtcg
     7381 accccgccgt gcttcgaacc cagcagctcc gcgaagtttt gcacgctcgc ttcactggtc
     7441 acgtccagct gatgaacgat cggttcgaga ccggccgcca ggagctgctc tcgggcggcg
     7501 ttgccgcgcg ccgcgtcgcg tgcagtcagg tacacgctgg catcttcgcc gagcgcgcgg
     7561 cacaacccct cgaccaacgc gaacccaagc ccttgattgg cccccgttac gatcgctact
     7621 ttcgccatgc attcctgttc tgagcaagcc gcccgttcgt cgagccagag ctccagctct
     7681 cgacgaacca tacaccaggc ccgttgactt cccacgttgc gtcgagtgtc ttcggcgcga
     7741 tgcgctcttc cttgctcacc caattcgcac caccgcgcgg ccgctcgaag gtggtttggc
     7801 tcgtgaccgt ttggcacgtg ggtgtggccg ggtgtctgcc cgcgccagac ccgaggcgtc
     7861 cagcaaaccc gacgagcagg cttgcatcgg ccacccccgc accaccgaag gggagcgaac
     7921 ccgcgacgag agcagcgggg tcgaatgtcc cttcgcgagc gggcgccccg tccgcttccg
     7981 caaccactgc cccagcgcct tcgaagacca gcgcttgcgc cgccaagacg gcggtgtctg
     8041 cgaatccgac cccgttcgag aaactgttcg acggacccgt atcttcgcta agcctcggta
     8101 aggcgcagcg cgcggtccta ctctccggcg ccgatgtgca cttgtacgac ggcaagcgac
     8161 tccgaaagct cgagcccgca gtaaacccta gcgcggacgg cctacacgcc aacgtcttct
     8221 tcgggcgcga caacgagccg cgcgtgctcg gatacataac cccatcccca accgcagccg
     8281 gagcccaatc cccgcgaacc aagtatctcc gttaccgcgg cggacgcctc gcggaggcca
     8341 aggacgaaat tggttcgctc gccagcgctc agggcgttct gtatggcgtg ctgggccatg
     8401 acgacccaga agtggtgtgt ctagcggaga agatctgtat cgtgaagcga ctcagcggtt
     8461 ggaaaaacat tcccgcccaa agcgctgcct tcgagatggt gctcgcccaa gggcaggtat
     8521 tcgcttggcg cgagaccacg ctcgagcagc tctcgaagac tgggttcgtg gcgctgccct
     8581 cgcctccaac ccgcgcgccg atccgctcgg tttggctcgg ccccgatcag gcactgtggc
     8641 tggtcgacgg cagcaccctt catcaacgtc gcgctgaaac ctggtcttcc ctcaccgcgc
     8701 caatacagaa tcccaccgtt gtttggggga gatcatcgaa cgacgtctgg ctgggcggga
     8761 gcgatggagc ggcgcacttc gacggccaat cttggcgctg cgtcgacggc gtccccggtc
     8821 ccatcaccca tatcgcgggc aacgagagca cgctctggat cgccggaccg ggcggcgcct
     8881 accgctccct cgcgagtgcc gcgggcacga gccgttagcg cgcctccctc gaggtagctt
     8941 ctcgcacact ccatacctgg gtgcgcctct ggccatttcg tacggcgccg catccagcgc
     9001 gacgcatccc gcacactata tatctagcgt gcacctcgcc cggtcggcgc cacgagacta
     9061 cgggcgcgac gctcgtctgc attcctctct ggcgcgcaca cggcggcgcg cagctcccca
     9121 cctcaccgcg ccggcgctct cttcatgaac catcgaggat gcgagacatt tcctgcggcg
     9181 ttcccttgcg gctcgggtcg cccgagtgat atacacactt tcccgtatgt cgatgtccca
     9241 ccgcctagag ctcgccgccc gcgccgcacg tcgtgctgcc cgcgctcgta gctcgggcgg
     9301 gctcgtcgag gcatgcggag acagtagata gaagaaccgc aaagcaagaa cgacgaggcc
     9361 gcccgaaaga ggcggccttt ttcgtttctg ggccgccgcg atcgggcgct ctcccaagcc
     9421 gatagctgaa tcatcgagaa ggagcgcccc catgcaacca ttcgacgcga gtcccacgtc
     9481 cgaggtgtcg tccccgtttt cgaacctcat gagcgcaccg ttgagcgctt cctccctcgc
     9541 cgccgatacc acgagcagcg ctcggggatc cgcgtcgacg ccgatcgacc accatcacat
     9601 ggtcgtcaac gaccggcccg cactgcgcat ggtgcgcggg gagggcagct atttgtggga
     9661 cgagcacggt aagcgctacc tggatttcat ccaaggctgg gccgtgaatt gtctcggcca
     9721 ctcaccaccc gtgatcgtag atcgggtgag ccgtcaaatg gcgcaagtgc tcaacgtggg
     9781 gccggcgttt tacaacgcac ccgcgctcga actctgtgaa cggctcgcac aggcgagcgg
     9841 gctggctcgc gtgttcttat cgaactctgg agcagaggcc aatgaaggcg ccgtgaagtt
     9901 gtgccgcaag tggggccaga aatatcgggg cggtgcctat caagtgatca cgaccgtaga
     9961 cggcttccac ggtcgcacgc tcgcgatgac gtgcgccacg ggcaaggcgg gctgggacga
    10021 agcgttcccg ccgcgggtag aaggcttcgt caaagctagc tacgggcaac tcgagccctt
    10081 gcggtcgttg attgggccac aaactgtcgc gatcctagtg gaacccatcc agggcgaagc
    10141 cggcgccgtg gtcccacccg acggctactt gaagggcctg cgcgagctgt gtgacgagca
    10201 tggcttgttg ttggtactcg atgaggtgca gacgggcttg gggcgaacgg gtccgctctt
    10261 cgcgttcgaa cacgaagggg tgcgaccaga cattctgacg ctgggcaaag ggcttggcgg
    10321 agggctcccg atttccgcgt tgctcgccag tgaaagggcg gcgtgcttcg aaaagggcga
    10381 tcacggcagt acgttcggag gcaacgcagt gatgagcagc gcggggctgg ccgtgctgga
    10441 tgagctcacc cgcgaaggcg cccgcgcgga gcgcgacgca tcttcggcgg tcttgtctcg
    10501 agtgttgcat gaactcgcgc gcgagctcgg ggccaccgtg cgcggacgcg ggcacctttt
    10561 cggtatgctg ttccgtcgag atgtcgcgct cacgctgcaa cgagttgcct tcgagttggg
    10621 cctcatcatc aacgcccccc gaccacacat cctacgcttc atgccagcgc tgaacgtcag
    10681 ccccgagcat tgcgaggaga tgagtaccct gttgcgcgaa gcggcgcgcc gcgtacttta
    10741 gggctcctgg gagctactcc acgaacttgg agaccagcct cacgtaagcg gcctgataat
    10801 aaatcgcagg ctcggtcagc tcccaggact tgtcataagg attcgatggg tcccagttgg
    10861 cgttggtgtc gacgtaagct ttctcgggcg gttggtcgcg cacgggggac ttggcgcagg
    10921 cgtgctgtgc agggtcctgc ccctgacagt agtctttgtt gggtcccccg gtgacgtagc
    10981 ctggagccgg accgaactcg ctcgtgcgcg cgttgtccca ttgcggcgaa ccgtcacgaa
    11041 accacgtgtg atacgcctcg tccgcgcatg cgtcgcctcc caaagcgtac atgttggtga
    11101 ggtaaaccag ctgcatcgcg ttcaccccgt gaaaggagtg caaaatgccg agcgcgcgct
    11161 cacggaagtc gcgctgacgc gatggctcca cgaggccgtg ttggacgaga tgatagttgg
    11221 tgttgccggt cgccgcgcgc gcgtagttgc taccccaatg ataggagctc gagcgcatgt
    11281 aggcccggta caaatcgagc tccggcttga atccgaacag gtccacactg ccggcgagtt
    11341 gcccaaggcg cgccaaaatt tcggctttga tcaacggggc tgccttgggc aggctcacgt
    11401 agtagagcag cgcgtccgcc tgactcggat cgtataccga ccaggtctcg tcctgaaagg
    11461 gccgggtgct cttgtagttg gcatccacga actcggcata tttgggctgc ccggttgcgg
    11521 cgaacagata aaccgcggcg gtcaccgccg cctgtagttg ttcggtcgag gaacggtctg
    11581 cgtcccctga cttgatggtg ccgtcgtcac aagcgtcgct cttcgggtgg gtctcgtagt
    11641 aggtgaacgc gcgcgccgca cgctgcacca gatcggcgga gtaggctttg aaacgcggga
    11701 aggccttcag caccagcgcc gcgtgcgcaa gctccgaggc gagcacgatc gtcgccgagg
    11761 agcacggctc cgggtagtag aagcgccgca gttggctctg atccggaacg ggatcgccat
    11821 actctacgtt tccgaccttg agcagcgccc cacccccgag atcggcgggt tgcatcttct
    11881 tgagccagtc gagctccacc acgagttcgt cgatcagatc gggcactccg ttgcccgatt
    11941 cagggatccc gaaatcgtca cgaaaggccg ccgggtgctc ggaatacgcg gtgagcagct
    12001 gatggacggc ctcacccgag aaggtaacgt acttgttggt atctccagca tcccaccagc
    12061 cgccgctcaa atcgcgcgcg gtcttcgcgt cttcacgcgc gcgcacgctg cgtgcttcgg
    12121 tgtcctggcc tgcgcccgcg tagtcccggg cttgcagcca gcacttcttg ccgaggcagg
    12181 cgtagggggc cactttcgcg aagttggcgc ggttgaagta gaacatcttc gtggcggctt
    12241 tgagcaccgc tcggtacacg tccttgtgaa tttcgaacgg atgcgagcgc accttccgct
    12301 cgggatcgta gacgtaatag gtgcctggaa cctggacgct gctgaagtcg aaccaggagc
    12361 ctcggtcgcc ggcactctcg tctagccgtc cctcgttcca gcgcgcaatc ttgccgcgga
    12421 acacgacgtt tccatcattg aagtttcgaa gttcgagctc gggggcgggc tcgtaacggt
    12481 gatcggcgcg ccagcccttc acgggctcca cgagcaccgc cactttggca ctctccgggg
    12541 tgtagccaaa ttggtcgatg accaggtagc gctcgaccgg caccgcggcc cctgccgcgg
    12601 cggcgggcaa aggcacgggt gcggggccct gcg
//
LOCUS       DASTKU010001015        10394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_420080, whole genome shotgun sequence.
ACCESSION   DASTKU010001015 DASTKU010000000
VERSION     DASTKU010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10394
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420080"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..511
                     /locus_tag="VFQ61_35970"
     CDS             314..511
                     /locus_tag="VFQ61_35970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HET9959963.1"
                     /translation="MSRKKISTTVYITPEQNERLHLLHSRTKVPVAVYIREGIDLVLA
                     RYEHVLPGQMSLDVPAKASKK"
     gene            646..2451
                     /gene="lepA"
                     /locus_tag="VFQ61_35975"
     CDS             646..2451
                     /gene="lepA"
                     /locus_tag="VFQ61_35975"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET9959964.1"
                     /translation="MAKTPDKIRNFSIIAHVDHGKSTLADRILDVTGAITERERVDQF
                     LDKLELERERGITIKAQSVRLRYRAKDGEEYQLQLIDTPGHVDFSYEVSSSLRACEGA
                     LLVVDATQGVQAQTVANAYLAIENHLEVVPVLNKVDLPSADVDRAAEQIEEVIGLDCS
                     GAVFASGKTGLGVPDVLEAVVQRIPPPKGDPNVPLRALLFDSWYDSYRGAVVMVRVME
                     GTLRKGERIKFMATGSTYDVTEIGCFAPHPTSLTELGPGEVGFLAANIKSIEDTKIGD
                     TVTLVYNAASDPLPGFREIKPMVFAGIFPTDSADYENLRDALGKLHLNDASFQFEPDT
                     SDALGFGFRCGFLGLLHMEIVQERLEREYDLDLITTAPSVVYFAYTKDGQTVRVDNPS
                     KMPAVGDIERIEEPILKLTIHVPSEYVGGVVALCEERRGIQLGLHYATKDHVVVTYEI
                     PLAEVILDFHDRLKSVSRGYASMDYEVQGYRADTLVKLDMLLNGDPLDALSVIVHRDT
                     AYTRGRALAAKLKELVPRQQYEVAIQAAIGSKVISRETVRALRKDVTAKCYGGDISRK
                     RKLLEKQKEGKRRMKSVGSVELPQDAFLAVLKLEG"
     gene            2644..4137
                     /locus_tag="VFQ61_35980"
     CDS             2644..4137
                     /locus_tag="VFQ61_35980"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9959965.1"
                     /translation="MQEATSDPPPSSAQPSSAGAPRDSERASNSARGAGVRNAKLLAR
                     QKLLSSVNAKAPEFGKVEHVETLPFGPNLRVERFRFSNGLQLLLCEDHSAPVLAYHTW
                     YRVGSRHERVGKTGLAHLFEHLMFNEVEGRPAGEFDRLLEEAGSEVNASTWLDWTQYN
                     AATPRDQLGLVVQLESERMSKLVLREPQVTSEKEVVANERRYRVEDDVEGSVSELLWA
                     TAYKEHAYRWPTIGWMEDIQNFTTADCEEFYKTYYAPNNATIVVVGDVTESQLLPKLS
                     RCYGVIAPSVIPLEDVRPEPPQLEERRCEVQKPTSTEKLVMGYHAPALGDYDHGILSL
                     LIEVLFGGRASRLHQLLVRELELVSEVRAFIGPFKDPGLVEIFASCREGHRAEEILER
                     IDAELTRVMEQPIAEEEIERGRTRFELSLLSGLDTMDGKASTLGFYDAVLGTPNVAFE
                     RLELIRRTGASELRRVARKYFDKRARSIIFVRPAPEGSNSSPAGDAT"
     gene            4134..5438
                     /locus_tag="VFQ61_35985"
     CDS             4134..5438
                     /locus_tag="VFQ61_35985"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET9959966.1"
                     /translation="MKRSTLASSSVLVETSHDLPLVSLSIGTKNGSLFDPPGLEGLTR
                     VTARLMRRTGGHRDPLEIDNRIDSLGGAFSTDVTASATLFQGTVIRRSLEPFLAIVRE
                     VLENPGLTEVELGRLVRETQADLVEALDDDRGLCRRWFRKRLFGDHPYSRPVSGTAES
                     LGRIRREDVVARARSLADPAALHVAFSGDIAADEATTLGTALLAALSEGEAPAETTPD
                     PVVPAGRRLVLVDKPARTQTQILIGGIGTHPRDPDHTALAVGNTIFGGTFTARMTQEV
                     RSKRGWSYGAYSSLPIDRRRQGFSMWTFPKAEDAAPCIALQLSMLSDLRKDGVTKKEL
                     SWAKRYLLRSHAFAVDTPAKRVGLALDKLLYDLPDGYYEEYKSRVSSVTLEEVNAALQ
                     QRIPEDDLLIVVVGTASDIRSAIEAQIPNLGSTEVVRFDNEV"
     gene            complement(5505..7265)
                     /locus_tag="VFQ61_35990"
     CDS             complement(5505..7265)
                     /locus_tag="VFQ61_35990"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET9959967.1"
                     /translation="MSVSPVNEIEERSVYARARAWADADPDPDTQRELRALIDGRATG
                     ELIERMGPELTFGTAGLRGRVEAGSARMNQAVVERTTWALAQHLISRVLDGRELSVVI
                     GYDARPHSRRFARAAAEVLIGAGVRVRWFDRAQPTPMVAYVARVMGAAAAIVVTASHN
                     PRGDNGFKVYGSDARQLDREDELAIEALRARFGEGHPVPRVSEGRLDARIEDVPDELF
                     ERYLAELDAYLPPRASNAAELKIAYTPVHGVGLLPTSSALSRRGFTTFNAVREQAMPD
                     GTFPTTPFPNPEHPGTLALVTQLARELDADLILANDPDADRLAAGARLSGGEWGILSG
                     NDLGVLLADFIFEYARPRRTPLFVTSIVSSPLLAELCRARGARVELTLTGFKWIWAAA
                     RALEAEGCEFVFGCEEALGYSVSPLVRDKDGVSAAVCLAELAARCRAQGSNLWGRLHG
                     IYREFGVYASAQRSMVRTGLEGQREIGSLLETLTSQAPTELLGDSVSEIIDYRQGAET
                     RPLWLGAASLVELKFASGTRLLARPSGTEPKLKIYADVRLGLSPRDSVSNVVSRARDR
                     AEALANALADYMQERAGLPG"
     gene            complement(7262..8650)
                     /locus_tag="VFQ61_35995"
     CDS             complement(7262..8650)
                     /locus_tag="VFQ61_35995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET9959968.1"
                     /translation="MFRRVLVLNRGEIACRIARTVRRLGSEAVMVYSDADRDALHTRA
                     GDEAYRLGAGPVRESYMNLEAVEAAVRTSAADAVHPGYGLLSENAELARTVTRAGAVF
                     IGPEPDTLELLGDKIRARALARAAGVEPPPGTANPVEPDAARLAQEAKQLGYPLIVKA
                     AAGGGGIGMAIVRTEEELLKSAKTCSDRALQAFADGRIYLERYLERPRHIEVQILADA
                     HGNVRALGERECSVQRRHQKVVEETPSPAAILSGAAGEAQREQLCQAAERIAHAANYR
                     GAGTVEFILDAHGTAFFLEVNARLQVEHPVTELVTGLDLVEQQLRIAGGEALSDAVRR
                     RAEGHAIELRVYAEDPARGFLPQPGRVVRFAWPEGAPGLRVDSGVAAGNEISTFYDPL
                     LAKVAAHGPDRETARQRLLQALSACEVEIQGPKGPRTTNLALLRAILAHPEFVSGEYD
                     TGLMGRMGSLPS"
     gene            complement(8695..9885)
                     /gene="rlmN"
                     /locus_tag="VFQ61_36000"
     CDS             complement(8695..9885)
                     /gene="rlmN"
                     /locus_tag="VFQ61_36000"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET9959969.1"
                     /translation="MSDVSTSPSPTLATNLEALGRFPEEWEATLSAQGQPKYRAKQVF
                     RWLHNKGVYAPAAMTDLSAGLRSHLESEGLGEPFRVAVVKRSSDTTRKLLLELSGGGN
                     VECVLIPMTKQVSDADAAAAVEDEEDVDAAPEKTKVTLCISTQFGCAMGCKFCASGRA
                     GLIRGLRAAEVAAQVLVAKRYVEPHEDLRNLVFMGMGEPLHHYDETARALRLLMHPDG
                     LGMSPRRITVSTVGLVPGIHKLGRDFDGKIGLAVSLHAPNDQLRSQIMPMNDRYPIAD
                     LMQALQDYPLPRRRRITIEYTLIGGVNDAIEHARELANLLRRLPVKINLIPMNPVANS
                     GFAAPEPARVAAFQAELARQGYSCFIRARRGDDVDAACGQLALASDLVGRSRLARASE
                     KPSS"
     gene            complement(9882..>10394)
                     /locus_tag="VFQ61_36005"
     CDS             complement(9882..>10394)
                     /locus_tag="VFQ61_36005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HET9959970.1"
                     /translation="VEKLVRRHPHVFADAQVKNSEEVLSNWEKIKATEKKQRPILDGL
                     PRSLPALQRAQRMSEKVSRVGFDWPDARGSRGKLDEEIAELDAALQAGDRREIEAELG
                     DVFFALVNLARHAGVDAEAALRGTCDRFSSRFAHVERRVKERHGGFEAPSPGLDVLDA
                     YWEEAKQAER"
BASE COUNT         1942 a         3334 c         3267 g         1851 t
ORIGIN      
        1 gctggttgct tggctgcgcg tccatgcttc ggccgaggcc gcgcggtcgt ggtccatgca
       61 ggaatgttcc aagccgttct cagcaaactg agagcataaa taccctgaat tacaaacaca
      121 tgagtccgct ctttaaatgc tctgctttcg aatgtgtcag ttcccgaatc ggcggcgtag
      181 cggcgcgcgc ggacgtgtag gagaaagtac gctgaagagt tgggttgaag aataacgtgc
      241 ggagcggccc cacaaatgac aacacaagta tgggaatcta gtgtaacgct gggtcccact
      301 tctctgagcg gtcatgtcgc ggaagaagat ctcgaccacg gtttacataa cgcccgaaca
      361 gaacgagcgt ttgcacctcc tgcactcacg cacgaaggtg ccggtggcgg tgtacatccg
      421 cgaagggatc gatctggtgc tcgcgcgcta cgaacacgtg ctgcccggcc agatgagcct
      481 ggacgtgccc gccaaggcct cgaagaagta agccgacgtc gtagaaataa gaaggcgtcg
      541 aaagagccag gaaggcgtcg tagaatgccg gagtcgggca cccagggcct gctgcgggtc
      601 gacgtttggc ttatttgagg ctaaagcccc gcccccacgg ttccgatggc caagactccg
      661 gacaaaatcc gcaacttctc gatcattgct cacgtcgatc acggcaagag cactctggca
      721 gaccgcatcc tggacgttac gggagcaatc acggagcgtg agcgcgtcga tcagttcctg
      781 gacaagttgg agctagagcg tgagcgcggc atcacgatca aagcgcagtc ggtgcgcttg
      841 cggtaccgcg ccaaagacgg cgaggaatac cagctccagc tgattgatac tcccggccac
      901 gtcgacttta gctacgaggt gtcgagcagc ctgcgcgcgt gcgagggagc cctgctcgtc
      961 gtggatgcga cccagggagt gcaggcccaa acggtagcaa acgcctacct ggcgatcgag
     1021 aaccacctcg aggtcgtgcc ggtcttgaac aaggtggatc ttccttccgc cgacgtagac
     1081 cgcgccgctg aacaaatcga agaggtgatc gggctcgact gcagcggcgc cgtgttcgcg
     1141 agcggtaaga ccgggctcgg ggtgccggat gtgctcgaag cggtcgtgca acgcattccg
     1201 ccgcccaaag gcgacccgaa cgttcctttg cgagcgctct tgttcgactc ttggtacgac
     1261 agctatcgcg gtgcggtagt gatggtgcgc gtgatggagg gcaccttacg aaagggtgaa
     1321 cgcatcaagt tcatggcgac cgggagcacg tacgacgtga ccgagatcgg ctgctttgcc
     1381 cctcacccca cgtcactgac cgagcttggg ccgggtgaag tcggatttct tgctgccaac
     1441 atcaagagca tcgaagacac caagatcggt gacaccgtca ctttggtcta caacgcagcg
     1501 tcggatccgt tacccgggtt ccgcgaaatc aagccgatgg tgttcgcggg catcttcccc
     1561 acggactcgg cggactacga aaacctgcgg gacgcgctcg gcaagctaca cctcaacgac
     1621 gcttcgttcc agttcgagcc ggacacgtcc gacgcgttgg gcttcggatt tcgctgcggc
     1681 tttttgggcc tgctgcacat ggaaatcgtg caggaacgcc tggagcgcga gtacgacctg
     1741 gatctgatca ccaccgcacc cagcgtggtg tacttcgctt acaccaagga cgggcaaacc
     1801 gtgcgggtgg acaatccgtc gaagatgccg gccgtgggcg atatcgagcg catcgaagag
     1861 ccaatcttga agctgaccat ccacgtgccc tcggagtacg tgggaggtgt tgttgcgctt
     1921 tgcgaagagc gacgcggaat ccagctcggt ctccactacg cgaccaaaga ccacgtcgtg
     1981 gtgacgtacg agatcccgct cgccgaagtg atcttggact ttcatgatcg cctcaagagc
     2041 gtgtcccggg gctacgcgag catggactac gaggttcagg gctaccgcgc ggatacgttg
     2101 gtcaagctcg acatgctttt gaacggtgac ccgctcgacg cgctgagcgt gatcgtgcat
     2161 cgagacaccg cgtatacgcg aggccgggcc ctggctgcga agctgaagga gctggttcct
     2221 cgccagcaat acgaggtcgc cattcaagcg gcgatcggct ccaaggtgat cagccgcgag
     2281 accgtgcgcg cattgcgcaa agacgtcacg gccaagtgct acggcggcga tatatcccgc
     2341 aagcggaagc tcctcgagaa acagaaagag ggtaagcgcc gcatgaagtc ggtcggcagc
     2401 gtggagctgc cgcaagacgc atttttggcc gttctgaagc tcgaaggctg agacggtgcg
     2461 tggtgacagc gcggcagcct ctgccgccgt gagttcgcga cggcgacgcg cgcgcggaat
     2521 gctccgagtg ccgcgcgcca actgtcaggc cgcacgagag cgcctttctt cacactagtg
     2581 ccgtggcacg cggcatgctc caacgcgctt ggcacgcacg gatccatggt atgcctgtgg
     2641 gccatgcaag aagccacgag cgaccctccc ccgtcgagcg cgcaaccttc gagtgccgga
     2701 gcgcctcgtg atagcgagcg ggccagcaac tccgcgcggg gtgccggcgt tcgaaacgcg
     2761 aagctcttgg cgcgccaaaa gctgctctcc agcgtgaatg ccaaagcacc cgagttcggc
     2821 aaggtcgagc acgtcgagac gctgcccttc ggcccaaacc tgcgcgtgga gcgcttccga
     2881 ttcagcaacg gcttgcagct tctattgtgc gaggatcact ccgcgccggt actcgcgtat
     2941 cacacctggt atcgcgtggg ttcgcggcac gagcgcgtgg gcaagaccgg cctcgcgcac
     3001 ctcttcgagc acctgatgtt caatgaggta gagggacgtc cggcgggcga gttcgatcgg
     3061 ctgctcgaag aagccggctc cgaagtgaat gcgtccacgt ggttggattg gacgcaatac
     3121 aacgcggcca caccgcgcga tcagcttggg ctcgtggtgc aactcgaatc cgagcgcatg
     3181 tccaagctcg tgctcaggga gccgcaggtc acgagtgaaa aggaagtagt cgcgaacgag
     3241 cgacgttacc gggtcgaaga cgacgtggag gggtcggtca gcgagctgct gtgggcgacg
     3301 gcgtacaagg aacacgccta ccgctggccc accatcggct ggatggagga catccagaac
     3361 ttcacgaccg cggactgcga ggagttctac aagacctact acgcgccgaa caacgcgacg
     3421 atcgtggtgg taggggacgt gacggagtcg cagctgctgc ccaagctcag ccgctgctac
     3481 ggcgtgatcg cgccgagcgt aattccgctg gaagacgtgc gacccgagcc gccccagctc
     3541 gaagagcgcc gctgcgaggt gcagaagccg acatccacgg agaagctcgt gatgggctat
     3601 cacgcgcctg cgctcggaga ctacgaccat ggcattctct cgctcttgat cgaggtgcta
     3661 ttcggcggtc gcgccagccg cctgcaccag ttgctcgtac gcgagctcga gctggtgagt
     3721 gaagttaggg cattcatcgg gccgttcaag gatccggggt tggtcgagat cttcgcgtca
     3781 tgtcgcgagg ggcaccgcgc cgaagagatt ctggagcgca tcgatgccga actcacccgc
     3841 gtgatggagc aaccgatcgc ggaggaagag atcgagcgcg ggcggacgcg cttcgagctg
     3901 agtctgctct cgggtctcga cacgatggat ggcaaggcgt cgacgctcgg cttttacgat
     3961 gcggttttgg gcactcccaa cgtcgccttc gagcgattgg aactgattcg gcgcacggga
     4021 gctagcgagc tccggcgcgt agcacgcaag tatttcgaca agcgggctcg ctcgatcatc
     4081 ttcgtgcggc ctgccccgga aggttcgaac tcgagccctg ctggagacgc gacttgaaac
     4141 gttcgacatt ggcttcgtcc tcggtgctgg tggaaaccag ccatgatttg cccctggtct
     4201 cgttgagcat tggcacgaag aacggctcgc tgttcgatcc tcccgggttg gaggggctca
     4261 cccgagtcac cgcccgcttg atgcgacgca ccggcgggca ccgcgatccc ctggagatcg
     4321 acaatcgcat cgactcgttg gggggagcgt tcagcacgga cgtgacggcc agcgccacat
     4381 tgttccaagg cacggtgatc cgacgctcgc tggagccgtt cctcgccatc gttcgagagg
     4441 tgctcgaaaa cccggggctg acggaggtcg aactcggcag gctcgtccgg gagactcagg
     4501 ccgacctggt cgaagcgctg gatgacgatc ggggcctgtg ccgacgctgg tttcgcaaac
     4561 gcctgttcgg agatcacccc tactcgcgtc ccgttagcgg caccgccgag agcctggggc
     4621 ggattcggcg cgaggacgtc gtagcgcgag cgcgcagctt ggctgatccc gcagctctcc
     4681 acgtcgcgtt ctcgggcgac atcgccgccg acgaagcgac cacgttgggc acggcccttc
     4741 tcgctgcctt gagcgaaggc gaggcgcccg cagaaactac gcctgatccg gtggttccgg
     4801 ccgggcggcg cctcgttttg gtagacaagc ctgcgcgcac gcaaacccaa atcctgatcg
     4861 gagggattgg aacccatcct cgtgacccgg atcacaccgc gcttgcggtg ggaaatacga
     4921 tcttcggcgg cacgttcacg gcccgaatga cgcaggaggt tcgctccaag cgcggttggt
     4981 cctacggagc atattcgagc ctgccgatcg atcgtcgccg tcagggattc agcatgtgga
     5041 ccttcccgaa ggcggaagac gcggcgccgt gcatcgcgct gcagctctcc atgctgagcg
     5101 acctccgcaa agatggcgtc acgaagaagg agctctcgtg ggccaaacgt tatctcttgc
     5161 gctcgcacgc gttcgcagtg gacacgcccg ccaagcgcgt agggctggcg ctggacaagt
     5221 tgctgtatga tttgccggac ggttattacg aggagtacaa atcgcgcgtt tccagcgtca
     5281 ctttggaaga ggtcaacgcc gcgctgcagc agcgaattcc agaggacgat ttgctgatcg
     5341 tagtagtcgg aacggcctct gacatccgca gcgcgatcga ggcgcagatc ccaaacctcg
     5401 ggtcgaccga agtcgttcgc ttcgataacg aggtttgaag gatcgacggg cgccgcttcc
     5461 cgtcggagcg cccatcgaat ctccgcacac cccttggagc gcgtctaccc ggggaggccg
     5521 gcgcgctctt gcatgtagtc ggccagcgca ttcgccaatg cctctgctct atcccgggcg
     5581 cggctcacga catttgacac cgaatctcgt ggactgagcc ccaagcgcac gtccgcgtag
     5641 atcttgagtt tgggctcggt tccgctcggg cgggcgagca atcgagtccc ggaggcgaac
     5701 tttagctcca ccaagcttgc agcccctagc caaaggggcc gcgtttccgc gccctggcgg
     5761 tagtcgatga tttcgctaac tgaatcgccg agaagctccg taggggcttg actggtgagg
     5821 gtctcgagca ggctgccgat ttcccgttgg ccttcgagac cggtgcgcac catcgagcgc
     5881 tgcgcgctcg cgtacacccc gaactctcgg tagatcccgt gcaagcgccc ccacaagtta
     5941 gacccttgcg cccggcagcg cgcggctagc tccgccaaac atacggccgc ggagaccccg
     6001 tccttatcgc gcacgagagg gctgacggag taccccagag cttcctcgca cccgaacacg
     6061 aactcacagc cttcggcttc tagcgccctc gcggccgccc aaatccactt gaagccggtc
     6121 aacgtgagct cgacccgtgc gccgcgcgcg cggcaaagct ccgcgagcaa cgggctcgag
     6181 acgatggagg tgacgaacaa aggtgtgcgc ctagggcgcg catactcgaa gatgaagtcc
     6241 gccagcaaga ctcccaagtc attgccactc aggatgcccc actcacctcc cgacaaacgc
     6301 gcgccggccg ccaaacgatc ggcatccgga tcattcgcaa ggatcagatc cgcgtcgagc
     6361 tcacgcgcca gctgagttac cagcgcgagc gttcccgggt gctccgggtt ggggaacggt
     6421 gtggtgggga aagtgccgtc aggcatcgcc tgttctcgga cggcgttgaa cgtcgtgaat
     6481 ccgcgccggg agagggcgct cgaagtgggg agcagaccca cgccgtgtac cggcgtgtag
     6541 gcgatcttca gctcggccgc gttgctcgcg cgcggaggca aataggcatc gagctccgcc
     6601 aagtatctct cgaacagttc gtccggaacg tcttcgatcc gagcatccag acgaccttcc
     6661 gaaacgcgag gtacggggtg accttccccg aatcgagcgc gtagcgcttc gatcgcaagc
     6721 tcgtcttcgc gatcgagttg ccgagcgtcc gagccataca ctttgaagcc gttgtcaccg
     6781 cgggggttat ggctggcggt caccacgatg gccgccgcgg cgcccatcac ccgtgcgacg
     6841 taggcgacca tgggcgtcgg ctgcgcgcgg tcgaaccaac gaacgcgcac ccccgcccca
     6901 atcagcacct ctgcggcggc ccgggcgaag cgccgactgt ggggccgcgc gtcgtacccg
     6961 atcacgaccg agagttcgcg accatcgagt acgcgcgaga tcagatgctg cgccaaggcc
     7021 cacgtcgtgc gctccacgac ggcctggttc atgcgcgctg agccggcttc cacacgccct
     7081 ctcagccctg cggtcccgaa cgtcaactcg gggcccattc gttcgatcag ctctccggtg
     7141 gctcgaccat cgatcaaagc gcggagctct cgctgggtgt ccggatccgg atccgcatcc
     7201 gcccaagcgc gggctcgtgc atacaccgat cgctcttcga tctcgttcac gggcgatacg
     7261 ctcaagacgg tagactcccc atgcgaccca ttagacccgt atcgtattca ccgctcacga
     7321 actcgggatg cgccaggatt gcccggagca aggcgaggtt cgtcgtgcgc ggccccttgg
     7381 ggccctgaat ctcgacctcg cacgcggaga gagcctgaag cagccgctga cgcgcagtct
     7441 cgcgatcggg accgtgagcc gcgaccttcg cgaggagcgg atcatagaaa gtcgagatct
     7501 cgttgccagc cgcgacgcca ctatccacgc gcagtccggg cgcgccttcc ggccaggcaa
     7561 agcgcactac cctgccgggt tgtgggagga acccccgcgc cggatcctcg gcatagaccc
     7621 gaagctcgat ggcgtgtcct tccgctctgc gtcggacggc gtcgctcaga gcctcaccgc
     7681 ccgcgatgcg cagctgctgc tcgacgagat ccaagccggt cactagctcc gtcaccgggt
     7741 gctcgacttg taagcgcgcg ttcacttcga ggaaaaatgc cgtcccgtgc gcgtcgagga
     7801 tgaactccac cgtgccggct ccgcgataat ttgcggcgtg cgcgattcgc tccgcggcct
     7861 gacacaactg ctcgcgctgg gcctcacctg ccgcgccact caagattgcg gccgggctcg
     7921 gcgtttcctc caccactttc tggtggcgtc gctgcacgct gcactcgcgc tcgccgagcg
     7981 cacgcacgtt cccgtgcgca tccgccagga tctgtacttc gatgtggcga ggccgctcga
     8041 ggtagcgctc cagataaatg cggccgtcgg cgaaggcctg cagcgcccga tcggagcacg
     8101 tcttggcgga cttcagtagc tcttcttccg tcctgacaat cgccatgccg attccgccac
     8161 ccccggccgc agccttgacg atcaagggat aacccaactg ctttgcttct tgagcgagcc
     8221 gcgctgcgtc cggctctact gggttggccg tgcccggagg tggctccacg cccgccgcgc
     8281 gcgccaaagc gcgagcgcga atcttgtctc cgagcagctc gagtgtatcg ggctcgggac
     8341 caatgaacac cgcacccgcg cgcgtgaccg tacgtgcgag ctccgcgttt tcactgagca
     8401 gcccgtatcc agggtgaacc gcatcggcgg cgctggtgcg cacggccgcc tcgaccgcct
     8461 cgagattcat gtaactctcg cgcaccgggc ccgcgccaag gcggtaagcc tcgtcccccg
     8521 ccctggtatg cagggcgtcc cgatctgcat ccgagtagac catcacggcc tcgctcccca
     8581 ggcggcggac ggtccgcgcg atccgacaag ctatctctcc gcgattcaga actagaaccc
     8641 gtcgaaacat cccttcactc tagctggcca gaagcgcgac cgcgcgcacc accttcaaga
     8701 gctcggcttt tcgctggcgc gagccagacg cgaacgtcca accaggtcac tcgccaacgc
     8761 cagttgacca cacgcggcgt cgacgtcgtc cccgcgccgg gctcgaatga aacagctgta
     8821 gccctggcgt gcgagctcgg cttgaaacgc cgccaccctt gccggttcag gcgccgcaaa
     8881 acccgagttg gccaccggat tcatgggaat cagattgatc ttgacgggga gccggcgaag
     8941 taaattggcc agctcgcgcg cgtgctcgat cgcatcattc acgccgccaa tcaacgtgta
     9001 ctcgatggtg atgcgccgac ggcgaggcaa cgggtaatcc tgcagtgcct gcatgagatc
     9061 ggcgatggga tagcgatcat tcatgggcat gatctgactg cgcagctggt cgttgggcgc
     9121 gtgaagcgac acggccaggc cgatcttgcc gtcgaaatcg cggccgagct tgtggatgcc
     9181 gggcaccaga cccacggtgc tgaccgtgat gcggcgtgga ctcatcccca ggccgtccgg
     9241 atgcatcaaa agccgcaacg cacgcgccgt ctcatcgtag tgatgcagag gctcgcccat
     9301 gcccatgaac accagattcc tcaggtcttc gtgcggctcg acgtagcgtt tggctaccag
     9361 tacctgggcc gcaacctctg cggcgcgcag accacggatt agcccggccc gcccgctcgc
     9421 acagaattta caacccatgg cacagccaaa ctgggtcgag atgcagagcg tgaccttggt
     9481 cttttcgggt gcagcgtcga cgtcttcctc gtcctcgacc gcggccgccg cgtcggcatc
     9541 actcacctgc ttggtcattg ggatgagcac gcactcgacg ttcccgcccc cgctcaactc
     9601 caagagcagc ttgcgcgtcg tatcgctgga acgcttgacc accgcaaccc gaaacggctc
     9661 acccaagcct tcgctctcga gatgcgagcg cagtccagcc gacaaatcag tcatggctgc
     9721 gggcgcatac acgcccttgt tgtgtagcca gcgaaatacc tgcttcgcgc ggtatttggg
     9781 ttgcccctgc gcgctcagcg tggcttccca ctcttcgggg aaacgcccca gtgcttcgag
     9841 gttagtcgcc agcgttgggc taggactggt gcttacgtcg ctcatcgttc ggcctgtttg
     9901 gcttcttccc agtatgcatc gagaacgtcg aggccgggtg aaggcgcctc gaatccgccg
     9961 tggcgctcct tcacgcgacg ctcgacgtgg gcgaagcgcg agctgaaacg gtcgcatgtt
    10021 ccgcgcagag cggcttcggc atccacgccc gcgtggcgcg ccagattgac gagcgcgaag
    10081 aacacgtccc cgagctcggc ctcgatttcg cggcgatctc cggcttgcag cgcggcgtcg
    10141 agctcggcga tttcttcgtc cagcttgcca cgcgagccgc gcgcgtcggg ccagtcgaaa
    10201 ccgacgcggc tcaccttttc gctcatgcgc tgggcgcgct ggagagcggg caaagagcgg
    10261 ggtaagccgt ccaagatcgg ccgctgcttt ttctccgtgg ctttgatctt ctcccagttg
    10321 gagagcacct cctcggagtt tttgacctgc gcgtcggcaa agacgtgggg atggcgccga
    10381 acgagcttct cgac
//
LOCUS       DASTKU010001016         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_420159, whole genome shotgun sequence.
ACCESSION   DASTKU010001016 DASTKU010000000
VERSION     DASTKU010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420159"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VFQ61_36010"
     CDS             complement(<1..896)
                     /locus_tag="VFQ61_36010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9959971.1"
                     /translation="MPSVPRASNEPASIAAPFERECDSELVGDRGNVVQDFLGMLVPT
                     SPEQSRPFHKPATWLDYFPLPAALLTRAGQIAAVNPELTRLVGEAAVSGRPFSDLLWP
                     ESREPALRHIAECGTSVDRVPIVTVTATGTRFALHVGMPLGQAASGMRVAALCSVEHA
                     LSSPRRSSIPAPPAFAEDPLRALSGLPLTALILADGRVTGITQRAAALLGIPESDFHP
                     VPLEDLLGTIAAAELFANCTGADSNWHPRALPAYPVRVEGSVRATASCLLPFGRPEVG
                     LLLLEGWRPSLEGSSSRASSAG"
     gene            complement(954..>2186)
                     /locus_tag="VFQ61_36015"
     CDS             complement(954..>2186)
                     /locus_tag="VFQ61_36015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET9959972.1"
                     /translation="VNEELQSVNEELHTVNAELQSKLQELSRVNADLDEVLATVDTGI
                     LVLDDDLKIRRFNQRATNFVRIVGHDAGRPLSHLSHSFAGTSILDDCARTAREHVGVE
                     RVLFSDDGARVLFRTKPIRSAGRDGVLVSFSDVTSIETLEDAAQRLNEAVEQLDSPVV
                     LLDKEGSIIYANRCFSDLSRRDAAFLPGTAFVDLLDESSRSGFAAALEQVRAGKPWHG
                     TSALDVPNSPPILEETRLRPLFSKTGSLIGVARFSAALPDLGRNVRILLVEDNAGDAM
                     LVEERLRLDGVINHFQWVTSAEAALERLDRSSAESLPDLILIDLGLPLISGEELLRIV
                     KSDVRYAHIAVAILTGADVAVREQALYDRGAVAIVRKPVDLDGFRQILRRLEGFWFSM
                     VRYGSPSQDELERSSKVE"
BASE COUNT          427 a          685 c          682 g          392 t
ORIGIN      
        1 tagccggcgc tgctcgcccg cgaagacgac ccttcgagtg aagggcgcca tccctccaac
       61 agcaggagcc caacttcggg gcgtccaaat gggaggagac aactcgcggt tgctcgaacc
      121 gacccctcga ctcgtaccgg gtaagccggc aaggcgcgcg ggtgccagtt ggagtccgcc
      181 cccgtgcaat tggcaaacaa ctcagcggcc gcgatcgtcc ctagcagatc ctcgagtgga
      241 accgggtgga agtcggactc gggtatcccg agcagggccg ccgcacgctg ggtaatgccc
      301 gtcacgcgcc cgtccgccaa gatcagcgcg gtgagcggca gcccggagag cgcccgcagc
      361 ggatcttccg cgaacgccgg gggagcagga atgctacttc gcctcgggga agatagcgcg
      421 tgctccacag agcagagcgc ggcgacgcgc atgcccgagg cggcttgacc aagtggcatg
      481 ccgacgtgca gggcgaagcg ggttcccgtc gcggtcacgg tgacgatggg gactcgatcg
      541 acgctcgttc cgcattcggc gatgtgacgc agcgccggtt ctcgggactc gggccataac
      601 aaatcagaga agggccgtcc gctgaccgca gcctcgccaa cgagccgcgt gagctcgggg
      661 ttcaccgcag cgatttggcc ggcgcgagtc agtaaggctg ccggcaacgg aaagtagtcg
      721 agccatgttg cgggcttgtg gaacggtcgg ctctgctccg gagatgtggg cacgagcatg
      781 cccaagaagt cttgcacgac gttaccccga tctccgacga gttcggaatc acactcacgt
      841 tcaaacggag ccgcaatgct cgcgggctcg ttgcttgctc gagggacgct cggcatcgtg
      901 atcctcgaca tcaatcaagc taatcgactg ccgcgtagca acacttaatc gcatcactcg
      961 actttcgagc tccgttcaag ctcatcttgc gagggtgagc catagcgcac catgctgaac
     1021 cagaacccct cgagacggcg cagaatctga cgaaaaccgt caagatcgac gggctttctc
     1081 actatcgcta cggccccgcg gtcatagagt gcttgttctc gcaccgcgac gtctgctccc
     1141 gtcaaaattg cgacggcgat gtgagcgtat cggacatcgc tcttgacgat tctgagtagc
     1201 tcttcaccgg aaatgagcgg cagacccaaa tcgatcagga tcagatctgg caggctctcc
     1261 gctgaagagc ggtccaatcg ttccaacgcc gcttctgcgg aggtcaccca ctggaagtga
     1321 ttgattacgc cgtcgagccg caagcgctct tctaccagca tcgcatcgcc tgcgttgtct
     1381 tctaccaaga ggatacgaac atttcggccc aggtcgggga gagccgcact gaagcgggcc
     1441 acgccgatca aactgcccgt cttggaaaag agcggtcgca accgtgtctc ttccaagatc
     1501 ggcggtgaat tcggaacgtc caacgccgac gtgccatgcc agggcttgcc cgcgcgcacc
     1561 tgctccaaag ccgcggcgaa gccactgcgg ctcgattcgt ccagaaggtc gacgaacgcg
     1621 gtcccgggca gaaacgccgc gtcgcgtcgc gagagatcgc tgaagcagcg attcgcgtag
     1681 atgatcgaac cttctttgtc gaggagcacc accggggagt cgagctgctc gaccgcctcg
     1741 ttcaagcgct gcgccgcgtc ttcgagagtt tcgatactgg tgacgtcact gaagctgacg
     1801 agcacgccgt cgcgaccggc tgaacggatt ggcttggttc ggaagagcac ccgcgcgcca
     1861 tcgtcgctga acagcacgcg ctcgaccccg acgtgctcgc gggccgtgcg cgcgcagtcg
     1921 tcgaggatcg acgtacctgc gaagctgtgg ctgaggtggc tgagagggcg tcccgcgtca
     1981 tgcccgacga tgcgtacgaa gttcgttgcg cgctgattga agcgccgaat cttgaggtca
     2041 tcgtccaaca ccaagattcc ggtgtctacg gtcgccagca cctcgtccag atccgcgttg
     2101 actcgcgaaa gttcttggag cttgctctgg agctctgcgt tcacggtgtg gagttcttcg
     2161 ttcacgcttt ggagctcctc gttgac
//
LOCUS       DASTKU010001017         9466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_420403, whole genome shotgun sequence.
ACCESSION   DASTKU010001017 DASTKU010000000
VERSION     DASTKU010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9466
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420403"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VFQ61_36020"
     CDS             complement(<1..681)
                     /locus_tag="VFQ61_36020"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9959973.1"
                     /translation="MSRCHVCAGPLHPGFALGRMPLGNGFLRPEQLPSEYFFELAVAP
                     CPECKLVQLENTVERERMFHEQYAYYSSTSRVMSEHFGEFARQVCATLRLREHPASGP
                     LVVEIGSNDGIFLQHVQAAGVRHLGVEPSANVGAVARERGIETWTEFFDAAVARRISE
                     SFGKAHAIVAANVMCHIPYVDSVLEGVRGLLERDGVFVFEDPYLPDILLNNAVDQIYD
                     EHTFYFSLH"
     gene            complement(678..1523)
                     /locus_tag="VFQ61_36025"
     CDS             complement(678..1523)
                     /locus_tag="VFQ61_36025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959974.1"
                     /translation="MTSVALSEAPDLDFEAHYVEHGFAILKRAVEPALCAELLDAVRH
                     WIGESRPLEEWTSDAPGQKYMVAYWGAYSAFDRLFEHPVINGHLADLFGRCGYQYQST
                     AENQPDRRRLALWVNPFDAEARPRLNRFGHIDSGSPYRGLSFQVCVADTEAHSGNTSF
                     FPGSHVPAHLAVLNNPDLAKSPGGILAHVPSGIDPFEFVAEAGDVALVHHGLVHSGNP
                     SHSKRRRPRIALRIEVFPEHPISVLDPSDPGRSAWERSFPVTERRELPEAIRYARETP
                     KEITV"
     gene            complement(1520..3004)
                     /locus_tag="VFQ61_36030"
     CDS             complement(1520..3004)
                     /locus_tag="VFQ61_36030"
                     /inference="COORDINATES: protein motif:HMM:NF015521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-hexose 2,3-dehydratase family protein"
                     /protein_id="HET9959975.1"
                     /translation="MALGLASRAQVATDEDLLSACLRSCAARDNVIGISNWLLQRAQR
                     DRLVARPTPLDSLAQWHFDSSTGFHHASGKFFSISGLRAELASARSVRSIEQPIIHQP
                     EVGVLGFIAKVSQGVLWLLAQCKDEPGNVSGPQLSPTVQATFSNYTRVHGGRAVPYVD
                     RFLEDGAGLRVFSQVQPEHGRYFAKKRNLNALMLVDDVELESERFRWVTLGELFWFLK
                     RDFGVSMDARSVLSCLLQELSQRFAVHLEGVSSSRANSVTGDGEFAHALRTSMFMRAG
                     ACHSDEWLKSWLNEQRATSSIRVTHMPLGELSTWSIDPLRVYSGQGHFHVAGFSVRAP
                     FRERDAWSQPLFCQNAREPSSPGARPVIGFVCQRIRSRLHFLCRSSLEPGEPDHVCIA
                     PTVVSRGEGTRETPFQDVALEDWLFDSRATVRLSANHSEEGGRFFRFVHEHRVVELPE
                     EVALDAPPGFAWLTLNQIYSLMASQRVTIEARNLLACLDPGEDA"
     gene            complement(3008..3481)
                     /locus_tag="VFQ61_36035"
     CDS             complement(3008..3481)
                     /locus_tag="VFQ61_36035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959976.1"
                     /translation="MTNSGSVGENSGALSGSGGESEVRKEFGTFATLETSQGASTYLL
                     SSQITYQLDKVGRRFKLNVYQGRGSFIDGIDLGPLHFRNRDTWESEIGAGGSIRVSGI
                     LSWIDGAQREAAVKLVMRATILENADLAYVTTVISGGPAGDQAATDLHIQMPASW"
     gene            4847..5956
                     /locus_tag="VFQ61_36040"
     CDS             4847..5956
                     /locus_tag="VFQ61_36040"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HET9959977.1"
                     /translation="MVLVCLSLVAALVLGTAFGKVEITVTSDGVVRAVTGVRPIRARL
                     NGAVVSVAASSGALVQVGTPIAVLDSTELEARHRRLEELVRGLRAKTDREQALSDELH
                     EKRMKGLQSMEATLRARLDLKRQVLGDQGVRTQQLKNLQGVGAASLHEVFGAREASHR
                     TREELFGMQERLATLNVEMADRQESHAQSSLTRHRALEEQETELREVEDLIRLAEIRS
                     PEGGRLESLTLTPGQVVEQGAVIAQLVPLDALRTAIVFVPAEEANFVAAGQAALVEFP
                     SLSVSEFGAAPATVTRVSSDVATPEEIVAALGPRPDSASRSMFRVELRLENSRLFDRM
                     QPHLRSGTLVRARLQTRTRRVASMLFDVVHNWIYR"
     gene            complement(6007..7131)
                     /locus_tag="VFQ61_36045"
     CDS             complement(6007..7131)
                     /locus_tag="VFQ61_36045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959978.1"
                     /translation="MADEIYQQSWSELASRSDLDGQVAEDQGVLRTVTAAPTARATLF
                     SGFRSELGKELTVVQRLDERRYRALPSRQRRAAFPPSVEDSVLSALGFAMSARPGRAA
                     LQTGIKVLDLLCPLPSTGTVAIFGGRSVGRTSLVYELHQRLARRSAGLVILFLVVPSD
                     AHLVAKVAEDEAGFPPDVQDGIQTFWLSSDRAQSREYASGSSFIDARIFLDASKAAQG
                     LFPAIDPLLSHSELLNTHELGARHIFLANSARSVLRESEQLITEFRFPRELAQAEFDA
                     AKAQREGAFQRAWMEGDVKQRSILQRARRLELYLTQTFYCAERFTQRPGTTVPLSAAL
                     DDLERILSGDLDTEPESSFLYVGSMFSPRPSGGIARFWHY"
     gene            complement(7166..7939)
                     /locus_tag="VFQ61_36050"
     CDS             complement(7166..7939)
                     /locus_tag="VFQ61_36050"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9959979.1"
                     /translation="MIDLRGKTVLVTGAAQGIGFGIAEVLAAAGAHVLVNDRRSGETH
                     AAVARLRDRGFSVEPAVADLTVPEQITQMVEQRVAPRQELHGLVNNAGIARFGGIEET
                     TLDTWNSIMDIQLRAHYLVTRAALPWLRAAGQASVTCISSVHSLLTVPTMLAYASAKG
                     AVIAMVRALSQELGPQGIRVNAVSPGFVDTPLFRGWLESEPDPAASLARVLDQIPLRR
                     IASPQDVGQLVAFLTSELALQLTGANLVLDGGLTTQLKH"
     gene            complement(7936..8418)
                     /locus_tag="VFQ61_36055"
     CDS             complement(7936..8418)
                     /locus_tag="VFQ61_36055"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET9959980.1"
                     /translation="MSELETDYLIQGLRGLAARGIRAPGVPFGLWREIGQLVPQPAVE
                     MIISNTGEDVLLTYRDDDYWSGWHIPGGFSGCGESLADTCQRLGRHELGADVRLLSVI
                     DSFAWPDHPYGNALSLLCHCELADPPGVGRFFAEPPTHMVRYHRDFMLKFQAWARRGR
                     "
     gene            complement(8422..>9466)
                     /locus_tag="VFQ61_36060"
     CDS             complement(8422..>9466)
                     /locus_tag="VFQ61_36060"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET9959981.1"
                     /translation="QARRVLANSKIRDAVITVRSQLAEQLFRASGSQEPLSLQLRHAY
                     GVLTPDHENVLRQAIGDGTLDRKHARRLAFNSELLTAKLADRRVEHRLTFESTLGVSP
                     PRPEGDRRWFSQEVLPLRLEGADFSLWIGNGMELWRGATARSKEPETIDWLRDNLEST
                     SVLYDVGANIGLYSLFAAAHPTRPTVFAFEPEPQNFARLAQNVQLNPGLSVIPFALAL
                     GVRRRFANFHSRTQTAGAASPAALQAGSGASVGVLVESLDNVVRGGAPIRPPTHLKID
                     VDGDEPEILRGAAETLHSAELKHVLVELRDAHVTETRDWLLASGFELAQHRDHGMDAD
                     GGRVGNFIFRKRS"
BASE COUNT         1866 a         2982 c         2868 g         1750 t
ORIGIN      
        1 gtgaagactg aagtagaagg tgtgctcgtc gtaaatttga tcgacggcgt tgttcaagag
       61 aatgtctggt aagtacgggt cttcgaacac gaacacgccg tctcgctcga gcagcccccg
      121 caccccttcg agcaccgaat cgacatacgg gatgtggcac atgacgttgg cagcaacgat
      181 ggcgtgagcc ttaccaaagg actcggatat gcgccgcgcc accgcggcgt cgaagaactc
      241 ggtccacgtc tcgatgcctc gctctcgggc caccgcgccg acgtttgccg atggctctac
      301 gccgaggtgg cgcacgccag cggcctgcac atgctggagg aagatgccgt cattgctccc
      361 aatctcgacc accaggggcc ctgacgctgg gtgctcgcgg agcctcagcg tggcgcagac
      421 ctggcgcgcg aactctccga agtgctcgct catcacccgc gaggtggagg agtagtaggc
      481 gtattgttcg tggaacatcc gctcgcgctc gacggtattt tcgagctgca ccagcttgca
      541 ctcggggcaa ggcgctaccg caagctcgaa gaagtactcg gacgggagct gttcgggtct
      601 caagaaaccg ttgcccaaag gcatgcgccc caaggcaaag cccggatgca acggcccagc
      661 acacacatgg caacggctca taccgtgatc tccttggggg tctcccgagc gtaacgaatc
      721 gcctccggga gttcgcgccg ctcggtcacg ggaaaggacc gctcccaggc gctccgtccc
      781 ggatccgaag gatccaacac gctaatgggg tgctcgggga agacttcgat acgtagggca
      841 atccgggggc gccgacgttt ggaatgtgat ggattaccgg agtgcaccag gccgtggtgc
      901 acgagggcaa catcacccgc ctccgcaacg aattcgaaag gatcgatgcc gctcggcacg
      961 tgcgccagga ttccgcctgg gctcttggcg aggtccgggt tattgagcac ggcaaggtgc
     1021 gcaggcacgt gactgccagg gaagaagctg gtgttacccg aatgcgcttc ggtatcagcc
     1081 acgcagactt gaaaggaaag cccccgataa ggggatccgg aatcgatgtg accaaatcgg
     1141 ttcagtcggg gtctggcctc ggcatcgaag gggttgaccc acagcgcaag cctgcgccga
     1201 tcaggctggt tttcggcggt gctttggtac tggtaaccac agcgcccaaa gagatccgcc
     1261 agatgaccgt tgatcacggg gtgttcgaat agacggtcga acgccgagta agcgccccag
     1321 taggcgacca tatatttctg gccgggcgca tcactcgtcc actcttccag cggcctggat
     1381 tcgccgatcc agtgccggac tgcgtcgagc agctcggcgc agagcgccgg ttccaccgca
     1441 cgtttcaaga tcgcgaagcc gtgctccacg taatgggcct cgaagtccaa atcaggggcc
     1501 tcgcttagtg cgactgatgt catgcatctt ctccgggatc caagcaggcc agtaagttgc
     1561 gcgcttcgat ggtcacccgc tggctggcca tcaacgagta gatctggttc agggtgagcc
     1621 aagcgaagcc gggcggggcg tcgagggcga cttcttccgg caactccacc acccggtgct
     1681 cgtgcacgaa gcgaaagaag cgcccgccct cttcgctatg gtttgcgctc agacggacgg
     1741 tggcccggct gtcgaacagc caatcttcca acgcgacatc ctgaaacgga gtctcccgcg
     1801 taccctcacc gcgcgagacc acggtgggag cgatacagac gtggtccggt tcgccaggtt
     1861 ccaagctgga gcggcaaagg aaatgcaaac gggagcgaat cctctggcat acgaagccaa
     1921 ttaccgggcg agcgccgggg ctgctgggct ctctcgcgtt ctgacaaaac agcggttgag
     1981 accaggcgtc gcgctctcga aacggagcgc gcacggaaaa tcctgcgaca tggaagtgcc
     2041 cctgaccgga gtagacccgc agcgggtcga tgctccaagt ggataactct ccgagcggca
     2101 tgtgcgtaac gcgaatggac gaagttgccc gctgctcgtt caaccaggat ttcagccatt
     2161 cgtcggagtg acaggctccg gcgcgcatga acatagaggt cctcaatgcg tgagcaaatt
     2221 caccatctcc ggtcacggag tttgctcgac tgctggatac cccctccaag tggactgcga
     2281 agcgctggct caactcctgg agtaggcatg agagcaccga gcgggcatcc atactgaccc
     2341 cgaagtctcg tttcaggaac caaaacagct cccccagtgt gacccaacgg aaacgctcgc
     2401 tttccagctc gacgtcatcc accagcatca aggcattcag attccgcttc ttcgcgaagt
     2461 accggccgtg ctcgggctgc acctgagaga acacacgcag gccggcgcca tcctcgagga
     2521 agcgatcgac atacgggacg gctcttccgc cgtgcactcg cgtatagttg ctgaaggtgg
     2581 cctgaacggt cggtgatagt tgcggaccgg acacgttgcc gggttcgtcc ttacactgcg
     2641 cgagtagcca cagaacgcct tgactcactt tcgcgatgaa tccgagtacc cccacctcgg
     2701 gctggtgaat gatgggctgt tcgatggatc ggacactgcg cgcggaggcc agctccgcac
     2761 gcagcccgga gatcgagaag aatttgccgc tcgcatggtg aaaccccgtg ctcgagtcga
     2821 agtgccattg agccaacgaa tccagcggtg tgggccgtgc caccaaccga tcgcgctgcg
     2881 ctctctgcag tagccaattc gaaatcccaa tcacgttgtc tcgcgccgcg caggaccgca
     2941 ggcaggcgct gagcagatct tcgtccgtcg cgacttgagc gcgactagcc agtccgagcg
     3001 ccatgaatca ccagctcgcc ggcatttgaa tatggagatc agttgccgcc tgatcaccgg
     3061 cgggtccacc gcttatcacc gtagtcacgt aagccagatc ggcattttcc agaatggtag
     3121 ctcgcattac gagtttgacc gccgcctcac gttgggcgcc gtcgatccag ctcaggattc
     3181 ccgagacgcg aatggatcct cccgcgccga tttcactctc ccaggtatcg cgattgcgaa
     3241 agtgaagggg cccgaggtct atcccgtcaa tgaagctgcc tcgcccctgg tacacgttca
     3301 atttgaatcg ccgccccacc ttgtccaatt gataggtaat ctgcgagctc agcagatacg
     3361 tagaggcccc ttgagacgtc tctaacgtcg caaaagtgcc aaactccttg cgcacctctg
     3421 attcaccccc ggaaccgctc agggcccccg agttctcacc aaccgacccc gaattggtca
     3481 ttcccatctc caacctggct gttttacagc tcgcaagaac gagcgatttg gacgaatatc
     3541 tagcgactca cacgcacacc cgcacgagac taggctcgtt cacgactcgg cagtttcctg
     3601 attaggtccc tcggcagtgt ccctcggcag tttccctcag caaaaggcgc gctgatatag
     3661 acatcgatct tcccgaacca gtgggttcgc ggcgagcggc cgtaaccttc gtattggcgc
     3721 ggacgaactc acccgcgagt tctggagtcg attaaatgca tacccgcctc ggtggaccag
     3781 taaccgtact agcccaccga ctttggggct cacaccgcct tgtgctttgc accaccacct
     3841 gcatcaccaa tatagcacca ccacctgctt taaccctggg cccaattaag catgacgctt
     3901 cgccgttgtc cagcagagtc gttgtgccgc gcgcattcac aagttggact ggcatatagt
     3961 ttcacttgtg cgccctctct ctcccgtcgg cgcgacgcgc tgttgccatc ctgtatcggc
     4021 attcggaaaa accccgccac cgtcgtggat tcactcgcga gacttcaact cacggttggc
     4081 gaggagcgcc cccccgagtg gctgccccta ggccgccgcg caatcagtga gacagcaatg
     4141 caacgaagct gccgagcagc cgtctgcgct cgaggttgct ggcgcgtgac agttggtgtc
     4201 agcgctgggc tcgcccctga gcggctcgaa tcagatctcc cggcctgcgt tcgaatctgc
     4261 tatgaacgcc ggtgaagggt agttgtgaca tggtctttgg ggcgaaccgt tttctgtttc
     4321 tctttggctc ggggcgagtc tcgatgttcc atgccgtggg gataaggata tgagcgagac
     4381 tcggaccaac gctgcgctcc tcgatacacg ccgcgcgccc gagccggacc tgcgccgaac
     4441 ccagccgtta ttccgaaact tcggggaacg ccccgcgcta cctggaacgc aagggccgga
     4501 cgtcagcgcg aggacgaatt cggcgcaaac ctcagcctct caatcgcctg ttagcgcagt
     4561 cgaatcccag ggtgctggag ccgctcctgc gggtacgtca aaggacaagg agccgccgac
     4621 cgcggtgcca ggtccctcat gggcgcccgg aaccgcagct tcgcaggctg cacctccgtg
     4681 gacgtcagtc acacctggcc cggcggcgcc gccaccttgg ccgtcgacgg caccctatcc
     4741 ccagaacgat gaccgggcac tgccattcag acaggccgcg ctccaggcac gggaggcccc
     4801 agaaccgatc gaacctctgc tggcgattga tacgagttcg tggtcggtgg tgctggtatg
     4861 cctctcactg gtcgcggcgc tggtgctcgg aacggctttt ggcaaagttg aaatcaccgt
     4921 cacgtccgac ggagtggtgc gcgccgtaac cggcgtccga cccattcgcg cccgcctcaa
     4981 cggcgcggtg gtcagcgtcg cggccagttc aggcgcgctc gtgcaggtag gcacgccgat
     5041 cgcggttctg gattccacgg agttggaagc tcgtcatcgt cgccttgagg agctcgtccg
     5101 gggcctgcgc gcgaaaacag accgtgagca ggcgctctcg gacgagctcc acgagaagag
     5161 aatgaagggg cttcaaagta tggaggcgac gctacgcgct cgcctcgatc tgaagcgaca
     5221 agtgctcggg gatcaaggcg taagaacgca gcagctcaag aatctccagg gcgtgggcgc
     5281 cgcctctttg cacgaggttt tcggtgcgcg tgaagcgtct caccgcaccc gtgaggagct
     5341 gtttggaatg caagagcgcc tcgcaacgct caatgtggag atggctgatc gccaagagag
     5401 tcacgctcag agctcgctca cgcgtcatcg ggcgctggag gagcaggaga ccgagctacg
     5461 cgaggtggag gatctgattc gactagccga aattcgttcg cctgaggggg gccggctcga
     5521 gtccctgacg ctgacgcccg gtcaggtcgt ggagcaggga gccgtgattg cgcagctggt
     5581 gccgttggat gctctgcgaa ccgcaatcgt gttcgtgccc gccgaggagg ccaacttcgt
     5641 ggccgccggg caagcggcgt tggttgaatt tccctctctg tcggtcagcg aattcggcgc
     5701 cgcgccggcc acggtaacgc gagtctccag cgacgttgcg acgcccgagg agatcgtcgc
     5761 cgcgctaggg ccgaggccgg actcggcctc gcgctcgatg ttccgggtcg agctgagatt
     5821 ggagaattca cgcttgttcg accgtatgca gccgcacttg cgctctggaa cactcgtgcg
     5881 cgcccgcctt caaactcgca cgcgccgtgt ggcgtccatg ctgttcgacg tggttcacaa
     5941 ttggatctac cggtgaccgt aggttttcgc tgcgaacgct cgcaccgaaa tagatatgga
     6001 ctgacgtcag taatgccaaa accgggctat gcccccggac ggtcgggggc taaacatcga
     6061 gcccacgtat agaaacgaac tctccggttc cgtatccaga tcacccgaaa ggatgcgctc
     6121 cagatcatcg agggctgcag acaagggtac tgttgtcccg ggccgctgag tgaagcgctc
     6181 cgcgcagtag aatgtctggg tcagatacaa ctctagacgt cgcgcacgtt gaagaatcga
     6241 gcgttgtttc acgtcgccct ccatccacgc acgctggaac gcgccttctc gttgggcctt
     6301 cgccgcatcg aattctgctt gtgcgagctc cctcggaaaa cgaaactccg tgatgagttg
     6361 ttccgattcc cgaagtacgg atcgtgccga gttggccaaa aaaatgtgcc gagctccgag
     6421 ctcatgggta ttgaggagct cggaatggct gagcaaagga tcgatagcag ggaacaggcc
     6481 ttgcgctgcc ttggaggcgt cgagaaaaat gcgcgcgtca atgaagctcg agccgctcgc
     6541 gtactcacgg ctctgcgctc tgtccgaaga taaccaaaac gtctggatgc cgtcctgaac
     6601 atcgggcgga aaaccggcct cgtcctccgc gacctttgcg accaagtgcg cgtccgatgg
     6661 cacgaccaga aacagaatga cgaggccggc cgatcttctg gccagtcgct gatgtagctc
     6721 gtagacaagg ctcgtgcggc ccacgctgcg cccaccaaaa atcgcgaccg tacccgtcga
     6781 aggcagtggg cacagcaaat ccagaacttt gattccggtt tgtagtgccg ctcgcccggg
     6841 ccgagcgctc atcgcaaagc cgagggcgct cagcacggaa tcctcaacgc tgggcgggaa
     6901 cgcggctcgg cgctgccggc tcggtaaggc acgataccgc ctttcgtcga ggcgctgcac
     6961 gaccgtgagt tccttgccga gttcggaacg gaatcccgag aacagggtcg cccgggccgt
     7021 gggagctgcg gtgaccgttc gaagcacgcc ctgatcttcg gcaacctggc cgtccaagtc
     7081 gcttcgactc gcgagctccg accagctctg ctgatatatc tcgtctgcca tgatcgctct
     7141 tcgctcgccg cgggtgggca tcgtttcagt gtttgagctg agtggtcagt ccgccgtcca
     7201 aaacgaggtt ggcgcccgtg agctgcagcg ccaattcgct cgtcagaaac gccaccagct
     7261 ggccaacgtc ttgtggggaa gcaatgcggc gcaacggaat ttggtccagc acgcgggcga
     7321 gtgaagccgc cggatccggc tcggactcca gccaacccct aaacaagggc gtatccacga
     7381 aaccagggct gaccgcgttc acacgaatcc cctgaggacc gagctcctgg gataaggccc
     7441 gcaccatcgc gatgaccgcg cccttggcac tggcgtaggc caacatggtc ggcaccgtca
     7501 gcagcgaatg aacgctggaa atgcaggtga cggaggcctg gccggcggcg cgcaaccagg
     7561 ggagagccgc ccgcgtgacc aggtagtggg ctcggagttg gatatccatt atcgaattcc
     7621 aagtgtccaa cgtggtctct tcgataccac cgaagcgagc aatccccgcg ttgttgacga
     7681 ggccatggag ctcctggcgc ggggctaccc gctgctcgac catttgggtg atttgctccg
     7741 gaacggtcaa gtccgcgacc gccggctcta cgctgaagcc ccgatcccgc aagcgagcaa
     7801 ctgcggcgtg ggtctcgccg gagcggcggt cattgacgag cacgtgggct cccgcggcgg
     7861 caagaacctc cgcaattcca aaaccaattc cctgcgccgc tcccgtcacg agtacggtct
     7921 tacccctcag gtcaatcatc gccctctcct cgcccaggcc tggaacttga gcatgaagtc
     7981 gcggtgatac cgaaccatgt gggtaggcgg ctcggcgaaa aagcgtccta cgcccggcgg
     8041 atcggcgagt tcgcagtggc acagcagaga gagcgcgttc ccataggggt gatcgggcca
     8101 agcaaaggag tcgatcaccg acagcaagcg cacgtctgca ccgagctcat gccgaccgag
     8161 ccgctgacac gtatcagcca gcgactcccc acagcccgaa aatcctccgg gaatatgcca
     8221 accgctccaa tagtcgtcat cccggtaggt gagcaacacg tcttcacccg tgttgctgat
     8281 gatcatttcg accgcgggtt gcggcaccag ttgcccaatc tctcgccaca gaccaaacgg
     8341 cacccctggg gctcggatcc cgcgcgcggc gagcccgcgc aggccctgga tcaagtagtc
     8401 ggtctcaagt tccgacattg gttagctcct cttcctgaat atgaagttgc ccactctgcc
     8461 gccgtcggcg tccatgccgt ggtcgcgatg ctgcgcgagt tcgaatcccg aagccaaaag
     8521 ccaatcgcgt gtttcggtga cgtgcgcgtc cctcaactcg accagcacat gcttcagctc
     8581 cgcgctgtgc agcgtttctg ctgccccccg taaaatttcc ggttcgtcgc catccacgtc
     8641 aatcttcaag tgcgttggcg ggcggatggg ggcgccgcct cgaaccacgt tgtccaggct
     8701 ctcgactagg accccgaccg acgccccaga tccggcctgg agcgcggctg gcgaggccgc
     8761 acccgcggtc tgggttcggc tgtggaagtt cgcgaagcga cgccgcacgc cgagagccag
     8821 cgcgaatggg atcacgctca gccctgggtt gagctgaaca ttctgtgcaa gccgcgcgaa
     8881 attctgaggc tcgggctcga acgcaaacac cgttggccga gtcgggtgtg cggccgcgaa
     8941 caagctgtac aagccgatgt tggcgccgac gtcgtacagt acgctcgtgc tctccaagtt
     9001 gtcgcgcaac caatcgattg tctccggctc cttggaacgg gcggttgcgc cgcgccagag
     9061 ctccatgccg ttgccgatcc acaatgagaa atcggctcct tccagacgca gtggcagcac
     9121 ctcttggctg aaccaacgcc gatcgccctc gggacgcggc ggcgagacac cgagtgtaga
     9181 ctcgaacgtc agcctatgtt cgaccctgcg atcagcaagc ttcgcggtga ggagctctga
     9241 attgaatgct aggcggcgcg catgcttccg gtccagtgtt ccgtctccga tcgcttgccg
     9301 caacacgttc tcgtgatcag gcgtgagcac gccgtacgca tgcctcagct ggaggctcag
     9361 gggttcttgg gagccgctgg ctctgaacag ctgctcggct agttgggagc gaaccgtgat
     9421 cacggcatcc cggatcttgg agttggccag cacccggcgc gcctga
//
LOCUS       DASTKU010001018        19028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_644404, whole genome shotgun sequence.
ACCESSION   DASTKU010001018 DASTKU010000000
VERSION     DASTKU010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 19028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19028
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644404"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..1080
                     /locus_tag="VFQ61_36065"
     CDS             466..1080
                     /locus_tag="VFQ61_36065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9959982.1"
                     /translation="MKHCSAEMEKSHRLSLVPMPKAGEPSALEARSDDELMLLARGGL
                     ERAFSVLIERHQARALRVALRYLGRESLAAEVAQDAFVEIFRALPQYQAHGKFPGYLY
                     RVLLNHCHMTWRRLRAERKAMLFAAEDVQEGDEAELLLRERRRDLHAALGEISEKLRS
                     VVLLRYSAGLSYEEIAETLDVPTGTVKRRLFDAMAKLRETLEGS"
     gene            1077..1919
                     /locus_tag="VFQ61_36070"
     CDS             1077..1919
                     /locus_tag="VFQ61_36070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959983.1"
                     /translation="MSRSPEEVRTLTAYLEGEVTASERAAIETKLQESAEARRTLAQL
                     ERVRELLSAPAVKLESIDLASRVRTRERELGPVVRTRPRRWQNLAYAGLTASLCAIAL
                     VLWSPHLASLRPGSWPPRKEDEGLRPKSNGAQLEGARWAGVHVFRVAGQAAPEPLGAR
                     LKSGDGLVFTYTNLGAHPFRYLMIFAVDASDQVHWFYPAYESVGEDPVSIPIAGGLAS
                     AALGELVQHDFAEGPLFVYALFTNVPARVLQVEAWLREQRRPSNEAPFVGGLLQRFDT
                     RIER"
     gene            1916..3463
                     /locus_tag="VFQ61_36075"
     CDS             1916..3463
                     /locus_tag="VFQ61_36075"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HET9959984.1"
                     /translation="MIWLRSLLALAVAIFLYSPTAPADTKGKGLRRFALVIGNNHPEA
                     AGTEPLRYADDDAVAAHLLLSEAGVDSSVFVTPDEGTRALHPSLQPAGRAGWAEVERG
                     FSSLMVRMRRAREGGFETEPLVFYSGHGDVANGEGYVVLEGGRLTRSRLFAMLAQSPA
                     SHNHVLVDACKSYYLVFDRGPGGRRSPYASSLAESLPSELRNTGFILSTSSARDSHEW
                     ERYQGGILSHQLRSGLRGAADANLDGIITYAELGAFLQTANAAIKNPRFKPDFMVRAP
                     NHELDRPILSFPASRGALLFTPRAWGHFYVENARGERLLDAHPTPEQPLRVQVPAERP
                     LFVRQNDERTEYVVSRQGSVAIAELSESRPEVAQRGALSLAFEWIFSTPFGQADVGAF
                     KRDAQLARDESPPRGPSSSLRPTLLWTSGLTALAAASAGLTLNGVALGTSLNARDTSQ
                     KNMAEKNQLIRKLNRASLPCYGVAVAAGLTWAFAKWWPESRVKFDGGSAELEREGALL
                     LRAGREF"
     gene            3467..6277
                     /locus_tag="VFQ61_36080"
     CDS             3467..6277
                     /locus_tag="VFQ61_36080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959985.1"
                     /translation="MLRDTLALTAVTLLSAAGCTLDLEGARCEELTCAAGYVCCPTDN
                     ICYRPERACGSAGAANTGGTSGGTSPTAGRGASQSSGAPAMGGSKAQGGASGLATAGN
                     STGGATQKSGDVEGGQGGDTSTGDSEHAYTFAVLSQHNDNGRSGVNPLETVLTRANVA
                     SGAFGELSRRALRGAVFAEPLLVPNVPGPAGSVNLVVVATMANVVYAFDADRPSSQPF
                     WVRSLEPPIELPDAQIPLGSDGIFHEVGILGTPVMSGDRLFVVTATRSGGTYRHWLHA
                     LDLRSGEVRSSVVIDDSGFDSAKHAQRAGLAYAEDTIYVAFGGYMANSDVGGWLFAYD
                     TRLNPRGSVSLGNPNGAAIHMNGQAPAIDPDASVYVTTGIDANTQDGISPDFSTRLLR
                     LDQPSTSGIVPLAPNPSELATTRLGRSGPLLVPGSNLAVVAEAGRVIASRRDAPGTDA
                     PPAQIFTVNRNEGCALALGCDSAPLPPVFWTGASSAGPGRLYTWLPNDQLRGFYYDSS
                     THEFRCPKEGTVCPPFAVSDLKDSSDSFSHPPERGAALSFSTNRDADGSSLVWAAHGY
                     TPTREKNYDGVVRAFDADTLSALWDTNSSETPLGPIAPGATPMVSGGRVYVGTADGLS
                     GQQSFSGNSNGGAPALLNFGDRYLVLSWGLSPTLTRGFELAFSSDGIHFDDTAIVPAS
                     FLNYEPALAADGASRLYLGFCSNSQKAEVLVSSDALFSSYESLGQRDSEGEIAPLELK
                     AASPLALAFGHGRLFMAWYDEAHLRFISSSDGIAFDLSTLREFPEFFAFHAPVLTYQD
                     GNLFLVSTTTDGSVHLLVSNDDGATFAEPITLPMSSNGHPALLWDTLTGADAPPSYTW
                     LWSAKSASTDGVLSASTGLAGDYTSFTGTRALGAIRTHPSISAARFKGAWHIAWLNLA
                     DSLPNVARYSSGELVTYGLKQP"
     gene            6393..7145
                     /locus_tag="VFQ61_36085"
     CDS             6393..7145
                     /locus_tag="VFQ61_36085"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9959986.1"
                     /translation="MDLKRHEPDLSELDAGWDFEAESYAVSAGEMDSLRPSYASLQPR
                     VEATRRAAPRSLPPLRAAALIVTVGLSGGALAAFALERPQLRAASTAAFHAAPTDHHR
                     PASPIPREPAPSAPPLADRVTLPKARRVLLTVEPPRANVIVHGRRVGASPVTLELGPN
                     ESASATVELPGYRTRVVEVDGTKPSLHIRLDSMPAPPRKKIASTAVRLEELPPSAAHS
                     EPAEKAESGPPSEANLAYPEPDAPLSPSPAAP"
     gene            complement(7225..7863)
                     /locus_tag="VFQ61_36090"
     CDS             complement(7225..7863)
                     /locus_tag="VFQ61_36090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959987.1"
                     /translation="MASLSVADAPAPTWRAAVRGYQRATLLALGALVTCLWSSSSQAC
                     GGGAVAPLNTQIGVNVQRILLSIKGGKTEVITQVAVPQTKALTINDLNGNILRAGGYS
                     AAVKDAVSNNKHRAFIVESATSRAQLQRSVPADVLELIDSNATVTRLSTVLPAEALTY
                     DVTLNVPLTSGVPNRRVLSWASQRQLRGVGLAMLLGVALVVRRGRRRAAERG"
     gene            8166..10529
                     /locus_tag="VFQ61_36095"
     CDS             8166..10529
                     /locus_tag="VFQ61_36095"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9959988.1"
                     /translation="MRVNSLVASVFVLASISAACGSGTLSAPDEILGTQSEAIFKGTV
                     ATTPFLRTGVERAVAKINGGGCTGTAIAADWVITARHCVNAAEEGVFVATISSHNVTF
                     EGSQFMDMIVPPENVFFFTLDGSDTALLKLPRTLGASFPTAKLFTGQDANAVGKAVKT
                     FGYGAFDKREDGLCATDSGKAQCAARSQGWGCNGDADCLRFDGQLRTANFKIDHIVKN
                     GEMYGNPVFDGKFINAPPNASGQYTLPGDSGGPTFINDGSAFQDQLVGTLGGGDNRAY
                     AKIFRDWVEGILINHTRGVLHPQYSLPFDWTSIQRASRKGDFAFGQWKAECGLGYVVS
                     GLSVDQTDSQPGPRAALCSYSPEADGGNSTMIDFSVSDARRDTSSGEWAGGFLKGECA
                     ADEAITALSQTTTTPPAGTKAKQLSRALCTPLKRPPSAARSCVVVDAEGSTVGVENDG
                     WAYNWGRTINGTPAPIGHYKAQCPDNYFMTGVSVSPSTGRPHSARCCYDFVPTDKQFV
                     FADVTLGGADLITVQPDSFDVQVSQGTRFDVSREFLYGGYRGKVGPQFADVTGDGRAD
                     LIISTDTGLVVRRSSGASFGSPEVWTDVAFEGKYGLFFADVDNDRDADAIVSNDFGIT
                     VRRSNGSRFLANEAWTTNAFLGSHGTFFADVDNDGDADAIVSNDFGLTVRRSDKTKFL
                     GNETWSDVAFLGTRGAFFADVDGDRRADAVVANDFGMTVRRSDGSRFTANEDWTSGPF
                     IGEFGNFLADVTGDGKADAIVINSFGVTVRRSLGSSFGPNEDWHLGS"
     gene            10797..11162
                     /locus_tag="VFQ61_36100"
     CDS             10797..11162
                     /locus_tag="VFQ61_36100"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET9959989.1"
                     /translation="MKYVCLVHFDPERLTAMNEREKQELDAKSLAFDQELSAGGHLIA
                     AEALQSPASAVLVKVRDGRLSTTDGPFAETKEHLGGFILIEARDLNDAIRIAAGIPLA
                     ALGTIEVRPIYEIPRPREQ"
     gene            11159..12385
                     /locus_tag="VFQ61_36105"
     CDS             11159..12385
                     /locus_tag="VFQ61_36105"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HET9959990.1"
                     /translation="MTQFDALYRTESRAVLATLVRLLGDLDLAEDAVHDAFRAAAEQW
                     PRDGVPEYPRAWLIATGRLSALARLRRRARFDRIQEQLAREIEARGAPNPEDRGLQDD
                     RLRLIFTCCHPTLGLDAQVALTLREVCGLTTEQIASCFVAKPSAIAQRIVRTKRKIRE
                     EQIPYEIPEPMELPGRLEVVLHVLYLVFNEGYRPSSGAEVTRVDLSSEAIELSRQLAT
                     LLPEPDALGLLALMLLHDARRPARTTARGETVLLADQDRSLWNREQIREGLALLARAW
                     SRPPVASYTIQAAIAGEHAIAADLPSTNWSKIVSLYDMLLVAENTPVVELNRAAAISE
                     ASGPEAGLRLMNELLARGMLDDYEPLHTARGELLRRLGRSAEAGVAYRRALELTKHAP
                     ERHFLEQRLKGLEVSC"
     gene            12379..12780
                     /locus_tag="VFQ61_36110"
     CDS             12379..12780
                     /locus_tag="VFQ61_36110"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET9959991.1"
                     /translation="MLRGQCYCGAVQFQVEDDFRYAFYCHCSRCRRRTGAACAAIAGI
                     DLEKLTITAGTEFVAQASVSDRGYRCICRKCCSPIYDALRNSAAGTVAHVQLGALSDT
                     PSRVPDHHIHVASKAPWHAIRDGLPQYDELP"
     gene            complement(12910..13773)
                     /locus_tag="VFQ61_36115"
     CDS             complement(12910..13773)
                     /locus_tag="VFQ61_36115"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9959992.1"
                     /translation="MAAPIDLNLLRAFATVHETGSFSAAAAKLGVPRSTVSRAVSALE
                     NLTGAALFHRTTRRVVTTDDGRALFDRVATQLASLDEALSDLPERREEPSGHLRVTST
                     VDLGVVVLAEVVTRFSARYPKVTTEVLLSPNVVDLAREKIEVALRVAPKSLPAGPYVA
                     RRVGVVSVQLFASPSYLARRGTPRSTSELGDHELVGYDRGSARVISEDMLFVREVLRK
                     HGGIGTLPSFLADEDVVGGHLVRVLPKHEMATARVYLAMPKHKHVPSRVAAFRDLVLE
                     VLRQRPLQTEE"
     gene            13913..14788
                     /locus_tag="VFQ61_36120"
     CDS             13913..14788
                     /locus_tag="VFQ61_36120"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET9959993.1"
                     /translation="MVNQTLLVTGASGHLGRLALGELLGRSPSSHLVATTRHPNSLAD
                     LAAKGVEVRYADFDDPASLEIAFRGLSRALLVSTDALATPGQRITQHTTAIRALLNAG
                     AEHIVYTSLPNPHGTPVLLGPDHAATEANLLASGIEYTILRNNIYADYLVPSLTEAVA
                     TGKYVNARGTGKVAWVTRQDCAGAAAGALVAARGRAVLDVTGPEPLSSADVAEIAAEL
                     AKKPIVHVSVSKEELKTAMLERGTPQPLAALFTSLDDGVEKGLFANVTDTVQRYAGRA
                     PLSVRSFLATSALAR"
     gene            complement(14839..15471)
                     /locus_tag="VFQ61_36125"
     CDS             complement(14839..15471)
                     /locus_tag="VFQ61_36125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959994.1"
                     /translation="MITRNGRLLALLSTIFSACGATSESDTASAEGPLSIVDAPGPLD
                     GCKSGAANFNGTLTSDGPDITALSNGGNYGTKLCDRFRVDVELECHGGPPRAVKIDGF
                     GTQRPSASDYSNCSDYREDITVYAGTVPNSLQRIGGGARHGEIVRIDGPPGAFLYGCK
                     VNDDDNFDPIPIPALCSGFINTYYRVTVRATRVSTGAKLPVQVLFDRAGN"
     gene            complement(15577..15843)
                     /locus_tag="VFQ61_36130"
     CDS             complement(15577..15843)
                     /locus_tag="VFQ61_36130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959995.1"
                     /translation="MVAISITSRSISQRRVQQRAQVLIHERNCSHLVLMEPASLSGTQ
                     PELVGATLYRREGPTLVLSTEGRRAAVGEAAGKTLMAAPADSGD"
     gene            15833..16414
                     /locus_tag="VFQ61_36135"
     CDS             15833..16414
                     /locus_tag="VFQ61_36135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9959996.1"
                     /translation="MATNRDLYTFIAGLVQSRTACGLALQEYLENLRRLGARLRDREA
                     ISLDEFEELLRSAFEPGPNADESHATPTPGYLAWEQRIAYQIRDLEEMRLAGTLENEH
                     RYFGVDAPRGARWYNFEPCTYLECAAAGSFGGWQEGDSTDRSYVPGPVAVLDAAGVIT
                     SVDPRDLENPVAELTQITWEMFLDFLDSGQCYE"
     gene            complement(16510..16932)
                     /locus_tag="VFQ61_36140"
     CDS             complement(16510..16932)
                     /locus_tag="VFQ61_36140"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9959997.1"
                     /translation="MKIFPYLAFPGTAEQAMKFYEKCLGGKLSEVHRFGTMPGSEQLP
                     EAVRNRVMHVGLALPDGFMIMASDTMEGMGPPYIAGTNVTVSIHPSSKAEADRVFAAL
                     AEGGQVTMPLADQFWGDYYGSLTDRFGIQWMVNFHAGS"
     gene            complement(17147..>19028)
                     /locus_tag="VFQ61_36145"
     CDS             complement(17147..>19028)
                     /locus_tag="VFQ61_36145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9959998.1"
                     /translation="LMIETVNDHAIFMLDPSGHVLTWSRGAEQIDGYTAEEIIGRHYS
                     CLFTPEDIASGLPARELELAAVEGRANIEGWRVRKNGSRFWANGTLAALYDNNHAVKG
                     FSEIARDSTAKRRNDELLRSVLNHTLDAIISIDERGTISTMNRAGELFFGRTASEVIG
                     QNVSLLMPEPYRHEHDDYLANYMRTGEAKVIGLGREVQGLRKDGATFPLELAVTEFRL
                     DDERQFVGILRDISEKRALEAQLRQSQKMEAFGQLAGGVAHDFNNLLTVISGSTEILL
                     TTIGEGDPARDDINAIQEAGERAAALTRQLLLFSRKAVLEMKVLDLNAEVSEMEKMLG
                     RMIGEDIALTTILGTRVGKIKADPGQLGQVLLNLAVNARDAMPDGGKLTIETQIVELD
                     EPYVDTHIEVEPGRYALLTVSDTGTGMPPEVKARIFEPFFTTKAIGNGTGLGLSVVHG
                     IVKQINGHIEVYSEVGIGTTFKIYLPSIEESAARAADPLEAMSVARANETILLVEDDD
                     KVRGIAMRALQSQGYHLLEAASGAEALQLLSAHAGGIDILLTDVVMPEMSGRQLTEAL
                     RPRFPQLKVLYLSGYTDDAVVRHGILEADVAFLQKPYTPTVLRRKVRQLLDRAEPPGS
                     PR"
BASE COUNT         3435 a         6190 c         5912 g         3491 t
ORIGIN      
        1 ttcctcgata tcgccgtcgt gagccccaac gccagcggcg cctggagcca atgggccacc
       61 atcgacagct ccaccggctc tgccttctcc tcctctcacc gcgatatcga cactccacaa
      121 cacatgcgta acggtgatcc gtagaagact gctgtgctcg ccgtcatcac cctccaccat
      181 cgaaagatgc tggagggtga tcccttttcg cgacggcaac gccagcgaag tagatccggg
      241 aaacaacgct tgcggcagcg cgctcactcg agcggcagat ccggtgccaa cggagctagc
      301 gtccggaacc cgtctgaaaa ggacttgctc ttcaacgcaa tcgaattcag acctgccgca
      361 acgcaagccc ccctccatcc ctttcaatgc gcgaccggcc cgtaaggttc ggtcgcttcc
      421 aacaaaaaga tccctgcggt cagagtgatc gaactttctg gcctcgtgaa gcattgttcc
      481 gccgaaatgg agaaatccca ccggctctcg ctggttccca tgccaaaggc tggggagccg
      541 agcgctctgg aagcgcgaag cgacgatgag ctgatgctgc tcgcgcgcgg cggtctggag
      601 cgcgccttca gcgtgctcat cgagcgccac caagcacggg cgctgcgcgt ggctttgcgc
      661 tatctgggac gcgagtcact ggccgcggag gtggctcagg acgccttcgt cgagatcttc
      721 cgggcgctcc cccaatacca agcgcatggc aaatttccgg gctatctcta tcgcgtgctc
      781 ctgaaccatt gtcacatgac gtggcgcagg ttgcgcgccg aacggaaggc gatgctcttc
      841 gctgccgagg acgtgcagga aggcgacgaa gccgagctcc tgctgcgtga gcgacgtcgc
      901 gacctgcacg cggccctcgg cgaaatttcg gagaagttac gaagcgtcgt tctgctgcgt
      961 tacagcgccg ggctgagcta cgaggaaatc gctgagacgc tcgatgttcc aacaggtacc
     1021 gtcaaacgcc gcctcttcga cgcgatggcc aagcttcgtg aaacgctgga ggggtcatga
     1081 gtcgatcgcc ggaagaagtg cgcacgctca ccgcctatct ggaaggtgaa gtcaccgcat
     1141 ccgagcgtgc agcgattgag acgaagctac aggagagcgc cgaagcccgg cgcacgctcg
     1201 cgcagctcga aagggtgcgc gagctgttgt ccgcgccggc ggtgaagctc gagagcatcg
     1261 atctagcttc ccgcgtccgc acgcgcgaac gagagctcgg ccccgtggtg agaacccggc
     1321 cgcgccgctg gcaaaacctc gcctacgctg ggctcacggc gagcctctgc gccattgcgc
     1381 tggtcctgtg gtcaccgcac ctagcttcct tgcgaccggg atcttggccg cctcgcaagg
     1441 aggatgaagg gcttcgcccc aagtcgaatg gcgcgcagct cgagggtgcg cgttgggccg
     1501 gagtacacgt gtttcgggtc gccgggcagg ccgcccctga gccacttgga gcgaggttga
     1561 agtcgggcga cggcctcgtc ttcacgtaca ccaacctcgg cgctcacccc tttcgatatc
     1621 tgatgatctt cgccgtcgac gcgagcgatc aagtgcattg gttctaccca gcctacgagt
     1681 ccgtgggcga ggatcccgtt tcgataccga tcgcgggggg gctcgcgagc gccgcgctcg
     1741 gcgaactcgt ccaacacgat ttcgcggagg gcccactgtt cgtgtatgcg ctattcacga
     1801 acgtgcccgc gcgcgtgcta caagtcgagg cgtggctgag agagcagcgc cgaccaagca
     1861 acgaggcgcc gttcgtcggt gggcttttgc agaggttcga tacacggatc gagcgatgat
     1921 ctggttgcgg tcgttgcttg cgctcgcggt tgcgatcttc ctctattctc cgacggcacc
     1981 ggccgacacc aagggcaagg ggctgcgccg cttcgcgctc gtgatcggca ataaccaccc
     2041 agaagcggcg ggcacagagc ccttgcgcta cgccgacgat gacgcggttg cagcccacct
     2101 tctgttgagc gaggccggcg tcgatagctc ggtgttcgtg acgcccgacg aaggcacgcg
     2161 cgcgctgcat ccctcgctgc agccggcggg gcgggcgggc tgggcggagg tggagcgcgg
     2221 cttctcgagc ctgatggtgc gcatgcgccg cgcccgtgag ggcgggttcg agaccgagcc
     2281 cctggtcttc tatagcgggc acggcgacgt cgcaaacggt gaaggctacg tagtgctcga
     2341 gggcgggcgg ttgacgcgct cgcgtctgtt cgcgatgctc gcgcagtccc cggcgtcgca
     2401 taaccacgtg ctcgtcgatg cctgcaaatc gtactacttg gtcttcgatc gggggccggg
     2461 ggggcgccgc tcaccgtacg cgagctccct ggccgagagc ttgccgtccg agctcaggaa
     2521 taccggcttc atactttcga cttcgtcggc ccgcgatagt cacgagtggg agcggtatca
     2581 gggcgggatt ttgagccatc agctgcgctc gggtctgcgt ggggccgcgg atgcgaacct
     2641 cgacggcatc ataacctacg cggagttggg tgccttcctg cagaccgcca acgccgccat
     2701 caagaacccg cgcttcaagc ccgacttcat ggtgcgagcg ccgaaccacg agctcgatcg
     2761 gccgatcctg agctttccgg cctcgcgcgg agcgctgctt tttacccccc gcgcctgggg
     2821 gcacttttac gtggaaaacg cgcgtggcga acgcttgctc gacgcccacc cgacccccga
     2881 acaacctttg cgcgtacagg tccccgcgga acgcccgctc ttcgtacgtc aaaatgacga
     2941 gcgcaccgag tacgtggtct cgcggcaggg cagcgtggcg atcgccgagc tctccgagtc
     3001 gcgaccggaa gtcgctcagc gcggcgcgct cagcttggcg ttcgaatgga tcttctcgac
     3061 accgttcggg caagccgacg tcggcgcgtt caagcgagac gcgcagctcg ctcgcgatga
     3121 gtcccccccg cgagggccat cgagctcact gcgccccacg ttgctctgga cctcggggct
     3181 caccgccctc gcggcggcga gcgccggact gaccctcaac ggggtggcgc tcggcacatc
     3241 gctcaacgcg cgcgatactt ctcagaaaaa catggccgaa aagaatcagc tgatccgcaa
     3301 gttgaatcgg gcgtccttgc cttgctacgg ggtcgcggtc gcagccgggc tcacgtgggc
     3361 gttcgccaaa tggtggccgg agtcgcgcgt gaagttcgac ggaggaagcg cggagctcga
     3421 gcgtgaaggt gcactcttgc tccgcgccgg acgagagttc tgagcgatgc tccgagacac
     3481 gctggcgctc acggccgtca ctttgctcag cgcagccggc tgtactctgg atctcgaggg
     3541 tgcgcgctgc gaagagctca cttgcgccgc cggctacgtt tgctgtccca ccgacaacat
     3601 ctgctaccgg cctgagcgtg cgtgcggctc cgcgggcgcc gcgaatacgg gcggcacttc
     3661 cggcggcact tcgccgacgg caggccgagg agcgagccag agcagcggcg cacccgcaat
     3721 gggaggaagc aaggcccaag gaggcgcgag cgggctggcc accgcgggca actcgacggg
     3781 cggcgcaacc cagaagtcgg gtgacgtaga gggcgggcaa ggaggcgata cctccacggg
     3841 tgattcggag catgcctaca ccttcgcggt gctgagccag cacaatgaca atggtcgcag
     3901 cggagtgaat cccctcgaga ccgtgttgac gcgagccaac gtcgcgagcg gagcattcgg
     3961 cgagctctcg cgacgtgcgt tacgtggcgc tgttttcgcg gagccgctgc tggttcccaa
     4021 tgttccgggg ccggccggct ccgtcaacct ggtagtcgtg gccacgatgg ccaacgtcgt
     4081 ctatgccttc gacgcggacc ggccgagttc acaacccttt tgggtacgga gcctcgagcc
     4141 acccatcgag ctccccgacg cgcagattcc gctgggcagc gacggcatct ttcacgaagt
     4201 cggaatcttg ggcacgcctg tcatgagcgg cgaccgactt ttcgtggtca cggccacacg
     4261 gagcggcggc acctatcggc actggctgca cgcgctcgac ctccgtagtg gtgaagtgag
     4321 atcgtcagtc gttattgacg acagcggttt cgacagcgcc aaacacgcgc agcgcgcggg
     4381 gctcgcctac gcggaagaca cgatctacgt cgccttcggt ggctacatgg cgaattcgga
     4441 cgttggcggc tggctgttcg cttacgacac acggctgaac ccgcgcggga gcgtctcact
     4501 cggcaacccc aacggcgccg caatccacat gaacggtcag gcgcccgcga tcgatccaga
     4561 cgccagtgtg tacgtgacga ctggtatcga tgcgaatact caggacggga tatcgccgga
     4621 cttcagcact cgcttgctca gactcgacca gccctcgacg agcggaatcg tgccgctagc
     4681 gcctaacccg tcggagctcg cgacgacccg gctcggccgc tcagggccac tgctagttcc
     4741 cggttcgaat ttggccgtcg tcgcggaggc tgggcgagtg attgcgtcgc gccgtgatgc
     4801 gcccggtact gacgccccgc ccgctcagat ctttacggtg aatcggaatg aagggtgcgc
     4861 gctcgccctc ggctgtgatt ccgcgccgct gccaccggtg ttttggacgg gcgcttccag
     4921 cgcaggcccc gggcgcctct atacctggct gccgaacgat caattacgcg gcttctacta
     4981 cgactcgagc acgcacgagt tccgctgtcc gaaagaaggc acggtgtgcc cacccttcgc
     5041 agtgagtgac ctcaaagact ccagcgacag cttctcgcat ccgcccgagc gcggcgccgc
     5101 gctgagcttc tccaccaatc gcgacgccga cggaagcagc ctcgtttggg ccgctcacgg
     5161 ctacactccg acccgcgaaa agaactacga tggggtggtg cgcgcgttcg acgcggacac
     5221 gttgagcgcg ctttgggata cgaacagcag cgagacgccg ctcggcccca tcgcgccggg
     5281 cgcgacgccg atggtgagcg gcggccgcgt ctacgtgggc acggccgacg gcctgagtgg
     5341 ccagcaaagc ttttcgggga atagcaacgg cggcgcgccg gcgctcttga acttcggcga
     5401 tcggtacttg gtcctgtctt ggggtctgtc gccgactttg acgcgcggtt tcgagctggc
     5461 gttctctagc gacggaattc acttcgacga caccgccatc gtacccgcct cgtttctcaa
     5521 ctatgaacct gcactcgccg ctgatggcgc atcacggctc tatcttggat tctgcagcaa
     5581 tagccaaaag gccgaggtgc tcgtgtcgag cgacgcgctg ttctccagct acgagagcct
     5641 cggtcagcgc gattcggagg gcgaaatcgc tcccctcgag ctgaaggcgg ccagcccgct
     5701 cgcgctcgct ttcggtcacg ggcgactctt catggcctgg tacgacgagg cacatctccg
     5761 tttcatttcg agtagcgacg gcattgcgtt cgatctctct acgctgcgtg agtttccaga
     5821 gttcttcgct ttccacgcgc cggtcctgac ttaccaagac ggcaatctgt ttctggtctc
     5881 gacgacgacg gacggctccg tccacctcct cgtatccaat gacgacggcg cgaccttcgc
     5941 ggagccgatc accttgccga tgtccagcaa cgggcacccg gcgttgctat gggacaccct
     6001 caccggagct gatgctccgc cctcgtacac ctggctttgg agcgcgaagt cggcaagcac
     6061 ggatggcgtg ctgagcgctt ccacggggct cgcgggcgac tatacgagct tcaccggcac
     6121 gcgcgcgctc ggggcgatcc gtacgcaccc ttccatttca gccgcgagat tcaaaggagc
     6181 ttggcacatt gcttggttga acctggccga cagcttgccc aacgtcgcgc gttacagctc
     6241 cggcgagctc gttacgtacg gcctgaaaca accgtgactc accgcgagac gtcgaattgt
     6301 gcgagctcga tcagaaaagc tacgggggcc gcccatctca ctggttggag aacgcgcccg
     6361 acagcgcgtc gtatcacttg cggaattctt acgtggatct caaacgccat gagccagacc
     6421 tttccgagct cgacgccggt tgggacttcg aagcggagag ctacgcggtg tcagcgggtg
     6481 agatggactc gctccgaccg agttatgcgt ctctgcaacc ccgggtcgaa gcgacgcggc
     6541 gcgctgcacc ccgctctctc ccgccgctcc gcgccgcagc gctgatcgtc acggtgggcc
     6601 tctcgggggg cgcgttggcc gcttttgcgc tcgagcgtcc gcagcttcgt gcggcatcga
     6661 cggcggcgtt ccacgccgct ccaacggacc accaccgtcc agcgtcgccc atcccccgcg
     6721 agccagcgcc ctccgctccg cccctcgccg atcgagtcac gctgcccaaa gctcgacggg
     6781 tgctgctgac ggtcgagccg ccacgcgcca acgtgatcgt tcacggacgg cgagtgggcg
     6841 catctccggt cacgctcgaa ctcggcccga atgagagcgc gtcagcgacg gtagagctgc
     6901 caggctatcg gacgcgggtc gtcgaagtcg acggcaccaa gccgtccctc cacattcgtc
     6961 tggattcgat gccggctccg ccccgaaaga agatcgcaag tacggcggtt cggctggagg
     7021 agctgcctcc ctcagcggct cactcggaac ctgccgagaa ggccgaaagc gggccgccta
     7081 gcgaagcgaa cctggcctat ccagagcccg atgcgcccct cagcccatcg ccggccgcgc
     7141 cctgaagtag gcgtcggctt tctcgtcgca gcggcgttgt tctgctggtc gcaggtcacg
     7201 accccacaca tccgcatcca ggcttcaccc acgctccgcg gcgcggcgtc gcccgcgtcg
     7261 aaccaccaag gcgacgccga gcagcatcgc gaggccgacg ccgcgtagct gccgctggct
     7321 cgcccaagaa agaacacgac gattgggcac tccactcgtc aatgggacgt tgagggtcac
     7381 gtcgtaagtc agggcctccg caggcaatac ggtgctcaaa cgggtgacgg tggcgttgct
     7441 atcgatgagt tcgagaacat cggctggcac gctccgttga agttgggcac gagaggtcgc
     7501 actttcgacg atgaacgccc gatgtttgtt gttgctcacc gcgtctttca ccgccgcgga
     7561 atacccgccc gcgcggagga tgttaccatt cagatcgttg atggtcagcg cttttgtctg
     7621 tggcaccgca acctgggtga taacttcggt cttgccgcct ttgatggaca gcaagatccg
     7681 ctgcacgttt actccgattt gtgtattcag cggcgcgacc gcgccgcccc cgcacgcctg
     7741 gctgctcgaa ctccacaggc aagtgaccag cgcacccaga gcaaggagtg ttgcgcgctg
     7801 atatccacga acggcagcgc gccaagtcgg agcgggagca tccgctacag ataaggaagc
     7861 catgcctatt cgtaacacac cgctcgtgac aggaggacca ctccatatgg tccagcacat
     7921 ctgagataga aaactgagcc aggtgagacc gcggatccgt attcgccggt ggcgtcgcgg
     7981 tggaccagtc gtgcggtaca cccgcggctg accagctgaa ccgcgtgggc ggtacgcacc
     8041 ccagtggcga ccgcaaaatc aggctgaact ctgcgcctat gggcatcacg agaagctggc
     8101 acgtgctgcg caatggtggc ggtccgaacc gatgcgccat gcaccggttc gcatggagag
     8161 tcagcatgag agtcaactct ttggtagcga gtgtattcgt tctcgcatcg attagcgccg
     8221 cgtgcggtag cggcaccctg agtgccccgg atgaaatcct cggcacgcaa tctgaggcca
     8281 tcttcaaggg cacggttgcg accacccctt tcttgcggac tggcgtcgag cgggccgtag
     8341 ccaagatcaa cggaggcggg tgcacgggca cggcgatcgc ggccgattgg gtgatcaccg
     8401 cccggcactg cgtcaatgcg gcagaggaag gcgtcttcgt ggctaccatc agcagtcaca
     8461 acgtgacttt cgaaggctcg caattcatgg acatgattgt tcccccggag aacgtcttct
     8521 tcttcacact cgacggctcc gacaccgcgc tcttgaagct accccgcacc ctcggcgcga
     8581 gcttccccac cgcgaagctg ttcactggcc aagacgccaa cgcggtaggc aaggccgtga
     8641 agacgttcgg ctatggcgcg ttcgacaaaa gggaggacgg gctttgcgcg actgactccg
     8701 gtaaggcgca gtgcgccgca cgaagccaag gttggggatg caacggagac gccgactgcc
     8761 tgcgcttcga cggccaattg cgcaccgcca acttcaagat cgaccatatc gtgaagaacg
     8821 gggagatgta cggcaacccc gttttcgatg gcaaattcat caatgcaccg ccgaatgcgt
     8881 cgggccaata cacgctgccg ggagactctg ggggacccac cttcatcaat gatggctccg
     8941 ctttccaaga ccagctggtg ggcaccctcg gcggcggaga caaccgagcc tacgccaaga
     9001 tattccgtga ttgggtggag ggcattctga ttaaccatac gcgaggtgtg ctgcatccgc
     9061 agtattcact ccctttcgac tggaccagca tccagcgcgc atcgcgcaag ggtgatttcg
     9121 cattcggtca atggaaggcg gagtgcggtc tcgggtacgt ggtcagcggg ctctcggtcg
     9181 atcaaacgga cagtcaacct ggtccacgcg cggcgctttg ctcttacagt cccgaagctg
     9241 acggcggcaa ctcgacaatg attgatttct cggtctccga cgcgcggcgc gatacctcgt
     9301 ccggcgaatg ggcgggaggc ttcctgaagg gagaatgtgc ggcagacgag gcgatcacgg
     9361 cactctctca aactacgacg acgccacctg cggggaccaa ggcaaagcag ctgagccgtg
     9421 ccttgtgcac gcccctgaag cggcccccca gcgccgcgcg ctcctgcgtc gtcgtcgacg
     9481 cagagggctc gaccgtgggc gtcgagaacg acgggtgggc atacaattgg gggcgcacga
     9541 tcaacggcac tcccgcgccc attggacact acaaggcgca atgccccgac aactacttca
     9601 tgaccggagt gagcgtctcg ccttccacgg gtaggcccca ttcggcgcgc tgctgctacg
     9661 atttcgtgcc caccgataag cagttcgtct tcgcggacgt cacgctcggc ggcgcagatc
     9721 tgatcacggt tcaaccggat agtttcgacg tgcaagtgag ccaggggacg cggttcgacg
     9781 tctcacggga gttcctgtac ggcggatatc gtgggaaggt tggcccccaa ttcgcagatg
     9841 tcacgggcga cggcagggcc gacctcatca tctccacgga tacaggcttg gtcgtgcgcc
     9901 gctcttcggg ggcttcgttc ggctcccccg aggtgtggac ggacgtggca ttcgaaggga
     9961 aatacgggct gttcttcgca gacgtcgaca atgaccgcga cgccgacgcc atcgtgtcga
    10021 acgattttgg catcacggtt cgccgttcga atggatctcg cttcctggcg aacgaggcat
    10081 ggaccaccaa tgcgttcctg ggttcgcacg gtaccttctt cgccgatgtc gacaacgatg
    10141 gcgacgccga cgcgatcgta tcgaacgact tcgggctcac cgttcgtcgc tccgacaaaa
    10201 ccaagttcct gggaaacgag acctggagtg atgttgcgtt cctgggaacc aggggtgcgt
    10261 tcttcgccga tgtcgacggc gatcgccggg ccgatgccgt ggtcgccaac gacttcggca
    10321 tgaccgttcg tcgctctgac ggctcgcgct tcacggccaa cgaggactgg acctcggggc
    10381 cgttcatcgg cgagttcggc aacttcctgg cggacgttac cggggacggc aaagccgacg
    10441 ctattgtcat caactctttc ggagtcacgg tcaggcgctc gctgggcagc tccttcggtc
    10501 caaacgagga ctggcacctc ggcagctgat tgcgttcgcg atgcggctcc cgtcttcgac
    10561 tcgggagccg cgtcgctgcg ctttcgcggc atggaaagct ggtcggttcc gagtgccgca
    10621 acgaccactg ctcacacgta cagtgactca cctcgcacgc tttgctggaa agaaaatcga
    10681 cggaaggtta acggcaaatg tcgatccgag gaggtctccg gcaactcatg gcggagcgac
    10741 ttgcctctca aaccggaatg aagcgctctc gcgcaccttt gctccagaag agacccatga
    10801 agtacgtctg cctagtccac ttcgatccag aaagactgac cgcgatgaac gagcgcgaga
    10861 agcaagagct cgacgctaag tccctcgctt tcgaccaaga gctgtccgct ggcggccacc
    10921 tgattgccgc ggaggcgctg cagtctcccg ccagcgccgt gctcgtcaaa gtgcgcgacg
    10981 gtcggctctc gaccacggac ggcccattcg ccgaaaccaa ggaacatctg ggtggcttca
    11041 tcctgatcga ggcacgggat ctgaacgacg cgatccgcat cgcggcaggg attcccctcg
    11101 ctgcactcgg taccatcgag gtgcgcccca tttacgagat ccctcgaccc cgagagcaat
    11161 gacccagttc gacgccctct accgaacgga gtcgcgggcc gtgctcgcca cgctggttcg
    11221 tctactcgga gatctcgatc tcgcggagga tgccgttcac gacgcattca gagctgctgc
    11281 cgagcagtgg ccccgggatg gggtgcccga gtacccacgg gcttggctga ttgcgacggg
    11341 aaggttgtcg gctctcgctc gcctgcggcg ccgcgcacgt ttcgatcgca ttcaagagca
    11401 gctcgcgcgc gagatcgaag cgcgtggcgc gccaaacccg gaggaccgcg ggctccaaga
    11461 cgatcgcctg cgcctgatct ttacgtgctg tcaccccaca ctcggtctcg atgctcaggt
    11521 cgcgctgacc ctgcgcgaag tgtgtgggtt aaccacggag caaatcgcgt cctgcttcgt
    11581 cgcaaagccc tccgccatcg ctcaacgcat cgtccgcacc aagcgcaaga tccgggagga
    11641 gcagattccg tacgaaatcc cggagcccat ggagcttccc ggtcggctgg aggtcgtgct
    11701 gcacgtgctc tatctcgtgt tcaacgaggg gtatcgaccg tcttcggggg cggaggtcac
    11761 gcgggtggat ctgagctcgg aagcgattga gctgtcccga caactcgcga ctcttcttcc
    11821 ggaacccgat gcgctggggc tgctcgcgct gatgctgttg cacgacgccc ggcgcccggc
    11881 gcgcaccacc gcgcgcgggg agaccgtgct gctcgccgat caagatcgct cgctgtggaa
    11941 tcgtgagcag atccgcgaag gtctcgctct gcttgcgcgc gcgtggtcgc gcccgcccgt
    12001 cgcgtcttac accattcagg ccgcgatcgc aggcgagcac gcgatcgcgg ccgacctgcc
    12061 gagcacgaac tggtcaaaaa tcgttagtct ctacgacatg ctgctggtcg cggaaaacac
    12121 gccggtcgtc gaactcaatc gggcggccgc gatcagcgaa gcttctggcc cggaggcggg
    12181 tctacggctg atgaatgagc tactcgcgcg cggcatgctc gacgactacg aacccctgca
    12241 caccgcgcgc ggagaactgc tgcgtcgact cggtcgtagt gcggaggccg gcgtggcgta
    12301 ccgtcgagcc ctcgagctga cgaagcatgc acccgaacgc cactttctcg aacaacgcct
    12361 gaagggcttg gaggtctcgt gttgagaggc cagtgctatt gcggggccgt gcagttccag
    12421 gtggaagacg actttcgcta cgccttctat tgccactgct ctcgctgccg ccggcgaacg
    12481 ggagccgcgt gcgcggccat cgccgggatc gatctcgaaa agctcaccat caccgccggc
    12541 accgagttcg tcgcgcaggc cagcgtgtcg gacaggggct atcggtgtat ctgtaggaag
    12601 tgttgctcgc cgatctacga tgcgctgcgc aacagcgctg ctggcaccgt agcgcacgtg
    12661 cagctcggcg cgctcagcga cacgccgtcg cgtgtgcctg accatcatat ccatgtggct
    12721 tccaaagcgc catggcacgc catccgagat gggctcccgc agtatgacga gctgccttga
    12781 actcgaaaag agttcacgcc agaatctctg caagagcctg cttgcgtgcc gaacgcgtcg
    12841 ttcttcaacg aaacgggcct tcattcgggc tccttcagat tcggtcgttc gggagcgtgg
    12901 tgtccggaat cattcctccg tttgcaacgg acgttgccgc agaacctcga gcaccagatc
    12961 ccggaacgcg gccacgcgcg acggcacgtg cttgtgcttg ggcatggcga gatagacgcg
    13021 ggcggtcgcc atctcgtgct tgggtagcac ccgtacgaga tggcccccca ccacgtcttc
    13081 gtcggcgagg aatgacggaa gtgtgccgat cccgccgtgc ttgcgcagga cttcacgcac
    13141 gaacagcatg tcctcggaga tgactctcgc cgagccgcga tcatagccga cgagctcgtg
    13201 atccccgagc tcgctcgtgc ttctcggcgt tccacgccgc gcgaggtacg acggtgaggc
    13261 aaatagctgc acggagacga cgccgacacg gcgcgcgaca tagggccccg caggcagaga
    13321 cttcggggcc acccgcaatg caacttcaat cttctctcgc gcgagatcga cgacgttggg
    13381 cgagagtagc acctcggtcg tcaccttcgg ataccgggct gagaaccggg tcacgacctc
    13441 ggcgagcacg acgacgccga gatcgacggt cgaggtcacg cgcaggtgac cactcggttc
    13501 ctcgcgccgc tctggaagat ccgagagcgc ctcatcgagg ctcgcaagct gggtggccac
    13561 ccgatcgaac agcgctcgac catcgtcggt ggtgaccacc ctgcgtgtgg ttcggtggaa
    13621 gagcgctgcg cccgtgaggt tttccagcgc ggagaccgcg cggctcaccg tggatcgtgg
    13681 cacgccgagc ttcgcggccg cggcggaaaa gctgccggtc tcgtgcacgg tcgcgaacgc
    13741 gcgtagcaag ttcaagtcga ttggtgcagc catgcaccag tgttgtcgct ttcgcgatat
    13801 tgtcgcaagc gtttcgatcc tgcacgatgt gcccactagg agcccagttg cggctctcat
    13861 tacggaactc aattgcggaa ctcaattgcg gagcccagcg aaacaacgag ccatggtgaa
    13921 tcaaactctt ctcgtcaccg gagcatccgg ccacctagga cgcctcgcgc tcggcgagct
    13981 cctcgggcgg agcccgtcta gccacctcgt ggcgaccacg cgtcacccga actcgcttgc
    14041 cgatctcgcg gccaaggggg tcgaggtgcg ttatgccgat ttcgacgacc ctgcgtcgtt
    14101 ggagatcgcc ttccgcggac tctcgcgcgc gctgctcgtg agcaccgacg cgctcgcgac
    14161 gcccggacag cgcatcactc agcacaccac cgcgatccgc gccctgctca acgccggcgc
    14221 ggagcatatc gtatacacgt ccctgcccaa cccgcacggc acccccgtcc tgcttggacc
    14281 cgatcacgcg gcaacggagg cgaacctgct cgcgtccggc atcgaataca cgattcttcg
    14341 caacaacatc tacgccgact acctcgtgcc ctccctcacg gaagcggtcg cgaccggcaa
    14401 gtacgtgaat gcgcggggca ccgggaaggt cgcctgggtg acacgccagg attgtgccgg
    14461 agccgctgcc ggcgcgctcg tagccgcccg aggcagggcc gtgctcgacg tgaccggccc
    14521 cgaaccactc tcgagcgccg acgtcgcgga aatcgccgct gagctcgcga agaagcccat
    14581 cgtgcacgtc tccgtttcga aggaagagct caaaaccgcg atgctcgagc gcggcacgcc
    14641 ccagccgctc gcggccttgt ttacatcgct ggacgatggc gtcgaaaagg gcctcttcgc
    14701 caacgtcacg gacacggtac agcgctacgc gggccgagcc ccgctgtccg ttcgctcatt
    14761 cttggcaacg agtgcgctcg cgcgatgaga cggcgagggg gcgtcggaca atctcccgga
    14821 ccggccccct ccaagcctct aattgcccgc gcgatcgaac agcacttgta cgggcaactt
    14881 cgcgccagtc gagaccctcg tggctcgcac cgtgacacga tagtaagtgt tgataaaacc
    14941 ggaacacagt gccgggatcg ggatcggatc gaagttgtcg tcgtcgttca ccttgcatcc
    15001 gtataagaac gcacccggcg gcccgtcgat tcgtactatc tccccgtgcc gcgcgccgcc
    15061 gccaatgcgc tggagcgagt tgggcacggt tcccgcgtat accgtgatgt cttcgcggta
    15121 gtcagagcag ttcgagtagt ccgatgcact cggtctttgc gttccaaaac cgtcgatttt
    15181 gacggcgcgc ggtggcccgc catgacactc gagttcgacg tcgaccctga accgatcaca
    15241 caatttggta ccatagttgc cgccattgct cagtgccgtg atgtccggcc catccgatgt
    15301 tagcgtaccg ttgaagttcg cggctccgct tttgcaccca tcgagtgggc ccggagcgtc
    15361 cacgatactg agtgggccct cggcgctggc cgtgtcggac tctgacgtgg ccccacatgc
    15421 cgagaagatg gtgctcaaca gcgctagcag acggccattt cgagtgatca tgcgtgcctc
    15481 cggtcaacga gtgtcttcgc accctcgaac cattcgacat cggttgtggc ccaagcggtg
    15541 ctcacgagcg agccccgtgc tcgctccgct cgtgacctaa tcacccgaat cggcgggcgc
    15601 tgccatgagt gtttttccgg ccgcctcccc caccgccgca cgccgacctt ccgtggaaag
    15661 aaccagcgta ggcccttcac gtcggtaaag cgtcgcgcca accagttcgg gttgggtacc
    15721 cgagagcgac gctggctcca tcagcacgag gtgactacag ttccgctcgt gaatgagaac
    15781 ctgcgcccgc tgctgcacgc ggcgctggga aatggatcga gaggtgattg agatggctac
    15841 caaccgcgac ctctacactt tcattgccgg cctggttcag agccgaacgg cgtgcggcct
    15901 ggcgctccag gagtacctcg agaacttgcg cagacttggc gcacgcctga gggatcgtga
    15961 ggcgatttcg ctcgacgagt tcgaggagct gctgcggagc gcattcgaac cgggccctaa
    16021 cgcggacgag tctcacgcaa ccccgacgcc cggctactta gcgtgggagc agcgcatcgc
    16081 atatcagatt cgtgacctcg aagagatgag actcgccggt actctcgaga acgagcatcg
    16141 ctacttcggc gtggacgccc cgcgcggggc ccgttggtac aacttcgaac cctgcactta
    16201 cctcgagtgc gcggccgcgg gctcgttcgg cggatggcaa gagggcgata gtaccgatcg
    16261 ctcgtacgta cccggacccg tcgcagtcct cgacgccgcg ggggtgatta cttccgtcga
    16321 cccgcgcgac ctggagaatc cggtagcgga gctcacgcag atcacatggg agatgtttct
    16381 ggacttcctc gattcgggtc aatgttacga gtagcgaagt tcacgcccgt actctccaag
    16441 gctttcagtt acgatgagcg ctactcacgg cgtagtagcg ctcaccgcaa cgagccgctg
    16501 gcgccttcct cagctccccg cgtggaagtt caccatccac tggatgccaa atcgatccgt
    16561 gagggagccg tagtagtcac cccagaattg gtccgcgagt ggcatcgtga cctgaccgcc
    16621 ctcggccaga gcggcgaaga ctcggtcggc ttcggctttg gacgaggggt ggatcgagac
    16681 cgtcacgttg gtaccagcga tgtagggcgg gcccatgcct tccattgtat cgcttgccat
    16741 gatcatgaag ccgtcgggca gcgcgagccc aacgtgcatc actcgattcc gcacggcttc
    16801 agggagctgt tcggacccgg gcatggtgcc gaagcggtgc acttcggaca gtttgccgcc
    16861 gagacacttt tcgtaaaact tcatggcctg ttcggcggtt cctgggaagg cgagatacgg
    16921 aaaaatcttc atgggtcttc ctccgccggg gaccatctca tggatccggc gtcaattggc
    16981 gctaaagatg cccgccccgc ccagaaccgc gaggcattcg cgaggcaatc cgcgacgccg
    17041 cgtcagaact cacgagttgc cgctgattgg ccgcgcggtc acttgcgcga ggttgctcac
    17101 gaggggttcg gcgtaaccaa gcaggcctga taggtaaacg atcgactcag cgggggcttc
    17161 ctggcggctc tgcccgatcc agcaactgac gcaccttacg gcgaaggacc gttggcgtat
    17221 acggcttttg caggaaggct acgtcagctt cgagaatgcc gtgccgaacc acggcgtcat
    17281 ccgtgtagcc gctcaggtaa agcaccttca gctgcggaaa gcgcggtcgc agggcctcgg
    17341 tgagttgacg accactcatc tccggcatca cgacatccgt caacaagatg tcaatcccgc
    17401 cagcgtgcgc gctcagtaac tgcagggcct ccgctccact cgcagcctcc agcaaatggt
    17461 agccctgcga ttgcagcgcg cgcatggcta ttccccgtac cttgtcgtca tcctcgacca
    17521 gcaggatcgt ttcattcgcg cgagcaacgc tcatggcctc caggggatcc gcggcgcgag
    17581 cggcactctc ctcgatcgac gggaggtaga tcttgaaggt cgtcccaatg ccgacctcgc
    17641 tatacacttc gatgtggcca ttgatctgct tcacgatccc gtgaaccact gacaacccca
    17701 accccgttcc attgccgatc gccttggtgg tgaagaacgg ttcgaagatg cgggccttca
    17761 cctcgggggg cattccggtt ccggtgtcgg acacggtcag aagagcatag cggccgggct
    17821 ccacttcgat atgagtatcg acgtacggct cgtccagttc gacgatctgc gtctctatgg
    17881 tcagcttgcc cccgtccggc atggcgtcgc gggcgttcac cgccaggttc agcagcactt
    17941 gtcccaactg ccccggatcg gccttgatct tccccacccg agtgccgagg attgtcgtca
    18001 gcgcgatatc ctccccgatc attcggccca acatcttctc catttcgctg acctccgcgt
    18061 tgaggtccag caccttcatc tccaacaccg ctttccggct gaacagcaga agctgtcggg
    18121 tcaatgccgc tgctcgttct cccgcctcct ggatggcatt gatgtcgtct cgcgcgggat
    18181 caccctcgcc aatcgtggtg agcaagatct cggtggagcc ggagataacg gtcagcaggt
    18241 tgttgaagtc gtgagcaact ccacccgcga gttgtccgaa tgcctccatc ttctgggact
    18301 gacgtagctg cgcctccagc gccctctttt cggagatgtc gcgcagaatt cccacgaatt
    18361 ggcgctcatc atccaggcgg aactcggtca cggccagctc gagcgggaag gtcgcgccgt
    18421 cctttcgcaa accttgcacc tcgcggccga ggccgatcac cttggcctct cccgttcgca
    18481 tatagttagc gaggtaatcg tcgtgctcgt gccggtatgg ctcgggcatg agcaagctga
    18541 cgttctggcc aatcacttcg gaagcggtgc gaccaaagaa cagctccccg gcgcggttca
    18601 tggtggagat agtgccccgc tcgtcgatgc tgatgatcgc gtcgagggtg tggttcagga
    18661 ctgaccgcaa cagctcgtcg ttgcgccgct tcgccgtcga atcgcgggca atctcgctga
    18721 agcctttcac ggcgtggttg ttgtcgtaca aggccgcgag cgtgccgttg gcccaaaacc
    18781 gggaaccgtt cttgcggact cgccagcctt cgatgttcgc ccggccctcg accgcagcga
    18841 gttccagttc tcgcgccggg aggccgctcg cgatgtcctc gggggtgaac agacaggaat
    18901 agtgtcgccc aatgatctcc tcggcggtat agccatcgat ttgctcggct ccgcggctcc
    18961 aggtcaggac gtggcctgaa ggatccagca tgaaaattgc gtgatcgttc acggtttcga
    19021 tcatcagg
//
LOCUS       DASTKU010001019         5706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1540139, whole genome shotgun sequence.
ACCESSION   DASTKU010001019 DASTKU010000000
VERSION     DASTKU010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540139"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFQ61_36150"
     CDS             complement(<1..265)
                     /locus_tag="VFQ61_36150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9959999.1"
                     /translation="MAGKRLTKAQVVAELANGSDLDKKSVGRVFESLQDLIRKQLSSR
                     GPGEFVVPGLVKLRVVKKPATKERQGINPFTKEPITIPAKPASK"
     gene            complement(720..1277)
                     /gene="ybeY"
                     /locus_tag="VFQ61_36155"
     CDS             complement(720..1277)
                     /gene="ybeY"
                     /locus_tag="VFQ61_36155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET9960000.1"
                     /translation="MSKALVRRLATDMLAELALSRAELSILLTDDEGIHELNLQHRKM
                     DKPTDVLAFPIDEKATEPERLLGDVVISIDTAERQASSRKRPLIAELRFLLAHGLLHL
                     IGYDHDTKPRKQRMDAMARRLVRSAPLPELFDASMPSPPATRSPRAPGRSARATRAPR
                     SRRAVPEAEKKPSRTKFARRKRHAV"
     gene            1646..2182
                     /locus_tag="VFQ61_36160"
     CDS             1646..2182
                     /locus_tag="VFQ61_36160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960001.1"
                     /translation="MTYRPAEVSFGPPFRTRIPSLLYLFIALSALMVVLVAERSAAGS
                     FLYVNIVERGVQGFIGARTMAFMLMLGALSSLVRTGMRGVRVRGDGVEFRDVVSFGMP
                     KLKRYRWAQIDRIVLDLPQSVAFDLWDGTRAFLPEVSDRSGLQAALEKVAAARAIPVR
                     GGLGLDEIPETNDFAEET"
     gene            2259..3320
                     /locus_tag="VFQ61_36165"
     CDS             2259..3320
                     /locus_tag="VFQ61_36165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9960002.1"
                     /translation="MKAKKPSRETNATLLKQAMLRRLPRRIAARGELLLAAVPSLLDH
                     YQQMLLSIFASLGRVFNDAEKAHMRTVLETWLNKGWEASPYARILVKYETDPPPKTTL
                     TYNVAVNVVTIADEYEHWTKTRTPPLFGAHADSKVMDLALAQGEPSSCPILDVGAGTG
                     RNTLPLLKAGFPTDAIEIAPALAAVLRNDAEQAGLNVRVFEGDATSRELGVPENHYQL
                     IVMAEVVASHFRNVAHLRQLLQALCAYLRPGGLLLFSAFLPLDGYKPDQLARELGEVF
                     WCVVFSRSDIEQAAAGLPLERIEDESTLEYEREHLPKEAWPPTGWFEQWSSGGDLFDL
                     PPQRAPLELRWLTYRKKLV"
     gene            complement(3391..4995)
                     /locus_tag="VFQ61_36170"
     CDS             complement(3391..4995)
                     /locus_tag="VFQ61_36170"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9960003.1"
                     /translation="MSAPSLGGSSAGKDAPKNPVQASDVDEAPRVLAGKTLGRYRLLY
                     PIAAGGMAQVWTARVEGGALARTVAIKLVRPEYAADEEYSRMFIDEAMVASSIHHPNV
                     CEIYELGRDNDVLFMALEWVAGDSLSGLVQRGSELRALPEPIAARIIADACAGLHAAH
                     EAVGPDGELLGIVHRDISPPNILVSMQGHVKVSDFGIAKARHQLHSRTRTGEIKGKFA
                     YIPPEQILGKGVDRRADVYAMGCVLYMATLGARPFGGGATALGKIVQGKYALPRELRS
                     DYPEALQKILVRALAADPNQRFRTADEMRQALEEWIMTQGALVMPTDIAGFVRQRMNP
                     EKRKLVESVLNKSRALPEAIAYQLLSVGERTDTPTATSGLALKPPRSKPISQPPDSDA
                     ATLVAPIPRNLAVSASNDARLPGAAGRALSSRPAPASTLPPAPGSIPTLKPPRQSSSG
                     LSSSASGLPGLVDPGSGPHDVRSAHEENGEHVGEASPPGVKATLKSIVPSLVPNFRGG
                     TTSSNVLLALGVAVALLATAYQLVVH"
     gene            complement(5480..>5706)
                     /locus_tag="VFQ61_36175"
     CDS             complement(5480..>5706)
                     /locus_tag="VFQ61_36175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960004.1"
                     /translation="TPHRDGLPLVGMPTLANDACALVSCHQRARAHDDVRGCVLGLHA
                     CGVRRRAATTACDDGVRPRVLRGNLGGADY"
BASE COUNT         1054 a         1789 c         1767 g         1096 t
ORIGIN      
        1 tcttgctcgc gggcttggcc gggatggtga tcggctcctt ggtgaacgga ttgattccct
       61 ggcgttcctt ggtcgctggc ttcttgacga cgcgcagctt gacgagacca ggaaccacga
      121 attcaccagg accccgcgac gagagctgct tgcggatgag atcctgcagc gactcgaaca
      181 cgcgacccac actcttcttg tccagatcag aaccgttagc gagctcagca acgacctgag
      241 ctttggtaag tctcttgcca gccatgagca tcctcctatg cgaaaaggct tttttcgacg
      301 agccgtgacg ccttctgtcc cgttcgaccg ccccggcagc ccgaaagcca ccgcgtcaac
      361 cgattcaggg ctgcgctcgg attaacaggt acggcgtggc ctaatcaagg ggaaagaatc
      421 agttgagggc tccgaagtgc ctttttttta gggccccgga gtgggggggt tccccgaata
      481 ccggtgggca cgctgaatcc gaagccaccc cacgcgggtt cccttgttcc atgcggctgc
      541 ggtagccact gcacatacat acaccatttt taacaaggaa ttccgcagct taacctcgca
      601 tctttggggc cgttctggag ctcgacaggc ggaactggac ccctggccgg caaggtcaac
      661 aaccgccttc tttgctcccg ttcgcgcgtt cccacgtcgc gtgcggacgc tcgtcgaact
      721 cagactgcgt gacgctttcg ccgcgcgaac ttggtgcgcg agggcttctt ttcggcttca
      781 gggactgcac gtcgggaccg tggcgcgcgc gtagcccggg cggagcgacc cggagcacgc
      841 gggcttcgag tagcgggtgg actcggcatg gatgcgtcaa aaagctcggg taaaggagca
      901 cttctcacca aacgccgagc catcgcatcc atgcgctgct tgcgtggctt ggtatcgtgg
      961 tcgtagccaa tgagatgcaa gaggccgtgc gccaatagaa agcgcagctc ggcaatcaat
     1021 ggccgcttgc gactcgaggc ttggcgctcc gcggtatcga tcgagatcac gacgtcaccc
     1081 agcaggcgct cgggttcagt cgccttttcg tcgatgggaa aagcgagcac atccgtgggc
     1141 ttgtccatct tacgatgctg gagattcagt tcgtgaatgc cctcgtcgtc ggtcagaaga
     1201 atgctcaact cggctcgact gagcgcgagc tcggcgagca tgtccgtcgc caaacgacga
     1261 accaatgctt tggacacggc agttgaggcg ggggcgcggc gcaagacgaa agcaggcaaa
     1321 gtgggaggct cctatcacgg ataaagaggc gggccgcggg tccaccgcag agcttgccca
     1381 gccgactcgg ggcaggaacg agactcatta acctcgagac cggggctgct ccagagctgg
     1441 atacgctccg gattcacatt tccagggctc gtagccagcg ctgcatactt tggttccgcg
     1501 ccctcttcat cgaagttcgg ccgcgaaatc cccgtgcttc gaccgaaccc gattcttcgt
     1561 cctaatctgt tcttcttccc aaggatgatg aggggagtta ggtttcagag gcatggctcg
     1621 gccgtcgcgc cctccgggtt tcgatatgac ttaccgcccg gcggaggtct ccttcggtcc
     1681 tccattcagg acgcgaattc cctcattgct ctacctgttc atcgcgctct cggcgctgat
     1741 ggtggttttg gttgcagagc gcagcgccgc cggcagcttt ctttacgtca atatcgtgga
     1801 gcgcggagtg cagggcttca tcggcgcccg caccatggcc ttcatgttga tgttgggggc
     1861 gctctcgtcc ctggttcgca cgggaatgcg cggcgttcga gtgcgcggtg acggcgtcga
     1921 gttccgcgac gtggtgtctt ttggaatgcc caagctcaag cgctaccgct gggcccaaat
     1981 agatcgcatt gtcctcgatt tgccgcaaag cgtggctttc gatctctggg atggcacgcg
     2041 ggcctttttg cccgaggtgt ccgaccgcag cgggctgcaa gccgcgctgg agaaggtcgc
     2101 ggctgcgcgc gcgattccgg tgcggggtgg gctcgggctg gacgagatcc ccgagaccaa
     2161 cgacttcgcg gaagagacct agagcctcgg tccagtcaag gcgcgccggc gattttacgg
     2221 cggtacatca ctgctctcgg tgatacggtc tggccgcgat gaaggccaag aagccgagcc
     2281 gagagaccaa cgctacgttg ttgaagcagg cgatgctgcg ccggttgccc cgaaggatcg
     2341 ccgcgcgagg cgagctgctc ttggccgcgg tgccgagctt gctcgaccac tatcaacaaa
     2401 tgctgctcag catcttcgct tcgcttgggc gcgtgttcaa cgacgcggag aaggcgcata
     2461 tgcgcacggt gctcgagact tggttgaaca agggctggga ggcctcgccg tacgctcgca
     2521 tcttggtgaa gtacgagacc gacccgcctc ccaagacaac gctgacttac aacgttgccg
     2581 tgaacgtggt gacgatcgcg gacgaatacg agcactggac gaaaacgcgg acgcctcccc
     2641 tattcggagc acacgctgac tcgaaggtca tggacctggc gttggcccaa ggcgagcctt
     2701 cgagttgccc gattctggat gtcggcgcgg gaaccgggcg gaacacccta cccttgctga
     2761 aggcgggatt cccaacggac gcgatcgaga ttgcgcccgc gttggcggcg gtgctgcgga
     2821 acgacgcgga gcaggccggg ctgaacgttc gcgtgttcga aggggacgca acgtcacgcg
     2881 agctcggcgt gccggagaat cactatcagt tgatcgtgat ggcggaggtg gtggcctccc
     2941 acttccgcaa cgtcgcccac ttgcggcaac tcctccaagc actgtgcgcc tatttgcgtc
     3001 cgggtggctt gttgttgttc agcgccttcc tcccactcga cggctacaag ccggaccagc
     3061 tcgcgcgaga gctcggggag gtgttctggt gcgtcgtatt ctcgcggagc gatatcgagc
     3121 aagcggccgc gggcttaccg ctcgaacgta tcgaagacga gtccacgctg gaatacgagc
     3181 gcgaacacct gccgaaagaa gcgtggcccc ccaccggctg gttcgaacaa tggtccagcg
     3241 gtggagattt gttcgatctc ccgccgcaac gagcgccatt ggagctgcgt tggctgacct
     3301 accgcaagaa gctggtctga tcccgcaacg aatgactggc cgcatccagg gctctgcact
     3361 cgtcgagccc cgcacaatca aacggcatgc tcagtgaacg acgagctggt acgcggtggc
     3421 gagcagagca accgcgaccc cgagcgccag aagcacgttg ctactcgtgg ttccaccacg
     3481 gaaattgggc accagcgagg gaacgatgct cttcagggtg gctttgacac cgggaggact
     3541 tgcttccccg acgtgctccc cgttctcttc gtgcgcggac cgcacatcgt gaggacccga
     3601 acccgggtcg accaggcctg gcaaccccga tgcggacgag ctcagccctg acgagctctg
     3661 cctcggtggc ttcaacgtcg gaatcgagcc cggggcgggc gggagagtgg acgcgggtgc
     3721 cggtcgacta ctcagagctc gaccggcggc gccgggtaac cgcgcgtcat tcgaggcgga
     3781 caccgccaag ttccgcggga tgggcgcgac gagcgtggcc gcgtccgagt ctggcggctg
     3841 agagattggc ttgctgcgcg gcggcttcaa ggcgaggccg ctggtcgcgg tgggcgtatc
     3901 ggtgcgctcg cccacgctga gcagctgata ggcaattgct tcgggaagag cgcggctctt
     3961 gttcagaacc gattccacga gcttgcgctt ctcggggttc attcgttgcc gtacgaagcc
     4021 ggcaatgtcc gtgggcatca ccaaggcgcc ctgcgtcatg atccactctt cgagcgcctg
     4081 ccgcatctcg tccgcggtgc ggaagcgctg attgggatcc gcagccaagg cgcgcaccaa
     4141 gatcttctgc agggcttccg ggtagtcact gcgcagttca cgcggcagcg catactttcc
     4201 ttgcacgatc ttgccgagtg cagtggcacc acctccaaac gggcgtgcgc cgagggtggc
     4261 catgtaaagg acgcacccca tcgcatagac gtcggcgcgg cgatccaccc ctttgcccag
     4321 aatctgctct ggcgggatgt aggcaaactt gcccttgatt tcaccggtgc gtgtccgtga
     4381 atgcagctga tggcgagcct tcgcgattcc gaagtcgctc accttcacgt gcccttgcat
     4441 ggacaccaga atgttcggcg gcgagatgtc tcgatggacg atcccgagca gctcaccgtc
     4501 gggcccaacg gcctcgtggg cagcatgcaa gcccgcgcaa gcgtcggcga tgatgcgagc
     4561 cgcgatgggc tcgggcaagg cgcgcagctc cgaaccacgc tgcaccagcc cgctcagcga
     4621 atctcccgcc acccactcga gcgccatgaa caacacgtcg ttgtcccgcc cgagctcgta
     4681 aatttcgcag acattcgggt gatgaatcga cgaggcaacc atggcctcgt cgatgaacat
     4741 ccgtgagtac tcctcgtctg ccgcgtactc cggacgcacc agtttgatcg cgaccgtgcg
     4801 cgcgagagcg ccgccttcca cgcgcgccgt ccacacttgt gccatcccac ctgccgcgat
     4861 gggataaagg aggcgatacc gcccgagcgt ctttccagcg agtacgcgag gcgcctcgtc
     4921 cacgtcggac gcttgcaccg gatttttcgg tgcatcttta cccgccgagc tgccacccaa
     4981 tgacggtgcg ctcatcgaag cgccggattt ttcgccggac tcttcgagcg gcggaatagt
     5041 ctgggctccg cgaccgtgca taacgtcact caatctactc gaatgagtcg ccgggatcca
     5101 gccactccga gagaaatggc gtacagcgca cacgcgagta cgtgcttgcg acacaactca
     5161 tcatgtgtgc tgcttccatt tgtttcccgt ccatgattcg acctcacgtc tcgcaactcg
     5221 agagcaggta ggttagaacc acaccgtttc ttacggccat tcccgcggca aaattcaaga
     5281 gaggagtttc gaagcgagaa cccccccaac cgcaagcaga tgccaagcct ccgattggga
     5341 ccgcacattt ctccgctcgt gagccgttct tggcgcggct tcttgcgcgc ggcagcttgc
     5401 gagtcagtca aacacgggag ttcgaactcg ctccgatagt ggcgaccaag agctacgttg
     5461 tttctatcac tctcaggtat tagtagtcgg cgccgcccag atttcctcgg agcacacgtg
     5521 gccgcacgcc gtcgtcgcac gccgtcgtcg cagcccgccg tcgcaccccg caggcatgta
     5581 atccaagcac gcacccgcga acgtcatcgt gagcccgagc gcgctgatga cacgagacca
     5641 gcgcacaggc atcatttgcc agcgtcggca tccctacgag cggcaagcca tcacggtgcg
     5701 gcgttc
//
LOCUS       DASTKU010001020        14682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_644868, whole genome shotgun sequence.
ACCESSION   DASTKU010001020 DASTKU010000000
VERSION     DASTKU010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14682
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644868"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VFQ61_36180"
     CDS             complement(<1..772)
                     /locus_tag="VFQ61_36180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960005.1"
                     /translation="MTSDPELEGAPAPHSSELNHAKLERLLRLLAALPEVGLRVTFLQ
                     GFVQEGPCDEVALLLDAMAARSARREPAVKAPMLVLAMLLASQCESRLVDDLREAAEL
                     LNLRHLERLVRRSPSSDVEPEARPSRIPDYGVGRELTLGERKWLARRPDRSAFARLLS
                     DPHPMVMEALLQNPRLTEDDVLRAAARRPANRDTQTCIARTGWLSRPRIRVALIHNPG
                     TPEGISVPLLSACTLPELREVITSPACPQLLRQLARELH"
     gene            879..3719
                     /locus_tag="VFQ61_36185"
     CDS             879..3719
                     /locus_tag="VFQ61_36185"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HET9960006.1"
                     /translation="MSDSSRVLRLAALFQEHLGGAPRRFSWALLLVAWVGAAHLARLG
                     TWTGRGAAVLVLVSSIGLAVALVRRERARLTGVPAVLRHVVWPIDPRLGQRASRAHEL
                     LVKTERDRQWGSMELAEHHFRGLLAKVPLTAVSEAARGRARRFLWGALACLGVMTALI
                     AVEPVRLLEGYDILAARGGVAPVPMPWFESAGIGVKPPAYLHLPEYALFMASDSALPE
                     GSTVKIRGLPVRPGRRLVLTDGQQELPFVEDGSGQVVAQLTLQRSVQLRIAARFGDVL
                     IYDPDEISLQIVPDQLPKVELVGAPRELALREVDHLELPYSVSDDHGIKQIDLVLRSA
                     GRVERRTLSRLDGQTLSEHGAHAIDARDSFFKRVFLPVRVTIEARDNEALHDDRWSAS
                     EAITLHPPGVGEPQALRMRGLRGAVGALIDALQSELALEAVKKGDLKSIRDAEARALG
                     MRKQALAELDQAFDATYASLSVASGLRAFLKGQLRTLAASRTDRKRRLEDVVLALDAA
                     LRAQAQRDAEDISRRLGDVAEEVAEAAKAALGSEERNARPRLDLALRVLDGGAENLLA
                     LGTLGADLGSVAQGELGRIRRALQARSLVDTELAARHLAARLRRPSPSFSSAGGGSVE
                     SGRGSSSGPPEGSEADREFDQMMRELAELTEQHADQIRGLEGILDQAERGQKDDQLSR
                     EAAERAERIREAASQLPEAAAALGPARAASARAREQLSNMAQNFERLDLKEAVAQGRA
                     ARGSLDEALRLQQDLQRGSDTREPPEQAAAREALGRELAWAEKQLQKLAEQAANRARE
                     GLAERSPREEGLARRAGNLAGRGEHSQAKLPEESVQALEQAENAMQRAANELAQGRGE
                     QALQLAREAQRLIEQSNPENGSDPQDSGDQRAESEQRAGKKGMATKAAVPGKDKGARA
                     EEFRRRVLEGLSKARRERLSKAVERYAEGLLE"
     gene            3716..5290
                     /locus_tag="VFQ61_36190"
     CDS             3716..5290
                     /locus_tag="VFQ61_36190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960007.1"
                     /translation="MRAIARLSAHVGLLASLLVGAPSSARLPDRDARLKRLADAITEI
                     DVAQAERLLSGLDSNAPEVAFESARLAIYRGDCGLARVILARPDLAETREGSELKNYA
                     EGCARATAAGFVVEDAKRGIWLRVQDAHDRALAPLLFDVAARARDAITRQVGVDLPKP
                     LRIDLVRDLFSLSAVSGLPLSAAETTGTLAVARWGKVILLSPRAAAHGFPWEDTLAHE
                     ITHLVVTRATRDHAPLWLQEGMAKRLETSWREPQPLDEPGWAEALAVRAFKEGRSVGI
                     DKLGASIAMLPTPEAASIAFAEVTSFVEYFVRQAGEPALRLLFADLRGVRSPDQALTS
                     VTGYPLPIWNQRWQAALGQIPETPERSPFVVEHARDVARRAVLSDLLLQRGHAVAALM
                     EVEPTVTVPTRPGASARATAAAPQRDPQLRHRAARAALLAGDGDRAAALLGTESEIAS
                     VHGPWFGVRGRLLRQHGDGVGAERAWSLGIAADPFAQETACEGQYSRTESPPETLPTD
                     PTRHELCLSTRQRPRD"
     gene            5771..6595
                     /gene="asd"
                     /locus_tag="VFQ61_36195"
     CDS             5771..6595
                     /gene="asd"
                     /locus_tag="VFQ61_36195"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241644.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HET9960008.1"
                     /translation="MSLSTFAAAQILRALPRVRISRAVGRLCERDLPKRVADPLLRAY
                     CRAYRVNLEEAEEASAGYPNFNAFFTRPLRQGVRRLSNDALVSPSDGLLSAAGRVDSG
                     ARLFVKGQHYEVGELIGDLNDAARYSGGQFAVVYLAPGDYHRVHSPAVGRVSLVRGIA
                     GDLYPVNRIGERHVPRLFVRNSRAVIPIDTAELGRITVVMVGAVIVGRITVTGLNFPD
                     VPPGEHTFDPAREVQPGDEIGIFHLGSTVVVLTEPGLSIARPLGRIRYGESLLRVE"
     gene            6621..9410
                     /locus_tag="VFQ61_36200"
     CDS             6621..9410
                     /locus_tag="VFQ61_36200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9960009.1"
                     /translation="MTIDELQDPTKTAAAVGSTAAAPVQEAVATPAPEPNAGSEEAPA
                     PATGLPAVSRKLAPPPKPNQRDSKPSFAPSGGEETPSPPAQVAAPRAPMIVIGGDPSP
                     QPARGAAQAWTRPASEPPPGRSEGSTPPPAGTTTSASADLRSKLGLSRPKPPPSQPNA
                     APAPNPAPLESRPAAARTPPAGRLLEMLLEANHDSPLRPNLPADSSSRIPIYDPESRG
                     AVSPTGAATQPGLAPVPPAPGDSSSMEGPPSSDWPDFEPLIAREPAGREPEKSGVASN
                     AAVSKPGPEAPLVPPPLLGGTEVVTQPGAVTQPMLATPTQAVPVVAPPVPAPPPLASS
                     AVVEPLAPPATASGAASASERRIPVATASAAANPNVVLAGSSVSMTSEPATSRVSKEA
                     EPRAERNGHEPAPEPVPGVVPKATPAAPLASMKMPALAPAVAAPSNPAPSASASVPAL
                     GSNEAAAPSKPKSEPARLRNTPRPRLTSDPSLRAAVPPRPAKPSPASPSFGDTAASSI
                     TGDSADAGETSDSGEDVPGPPRVPRFALNASATSTPLPVPSGGRRSDPPVPASAIAAM
                     KIIAVGDDEAALASNLEQVVEIEEDVPIPPDLAAEIPIDVEPPHEDVEEVAVDEVAPQ
                     EGRQRADSIPPVDVVIEEAEAEQTLRRPGEDVKPPPPPLRRPKTPSIPRAVPQAKRRQ
                     KPAWWEEVFQEDFMRAAFRLTDEQLRAEVDFIEESLGVASGGVVLDLGCGAGYHAVEL
                     ASRGYGVVGYDLSLYQLALASDVAQERGQKLNFLQGDMREMAFEEMFDGIFSWNTSFG
                     YFEEEKNVHVAERVFRALRPGGMFLIDVLNRDYAAVSSPCQLWYEGDACVCMDDMTLD
                     FITSRLRVKRSVILDDGRTREAVYSIRLYSLHELGKLLHDVGFRVTEASGHPATPGVF
                     FGEYSPRIIMLAQKP"
     gene            complement(9411..9929)
                     /locus_tag="VFQ61_36205"
     CDS             complement(9411..9929)
                     /locus_tag="VFQ61_36205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960010.1"
                     /translation="MPRSAALVSAAIECPPALSARGLRGLFTRDSLRISAVVLLLRAR
                     SWVVSLGMVPCAALTIGCGGGAAKSEPSSEHIASSDTRARHAESSASSEDEASEDAAP
                     EAPCTDGTCFSCGKGYCPSGWYCDESAAGGAACAWLPACNGKNQCRCLEQTLGAECSC
                     SDADSAARVTCK"
     gene            9972..12050
                     /locus_tag="VFQ61_36210"
     CDS             9972..12050
                     /locus_tag="VFQ61_36210"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET9960011.1"
                     /translation="MNLDQALKELAPRAKVSQSPADLAAYAGDLWPRGLVQLQQDTRS
                     ERAPLGVVWPESAVEVARILQFARDEGLEIVPFGAGSGVAGGILPGARSLVLDMKRLS
                     DFGVEAGPTLNVGAGALGITLEERLLEQGFTTGHYPSSILCSTVGGWVAARGIGQCSG
                     RYGKIEDMVTRLEVVLSDGEVVELHSRQRGPDLVPLFVGSEGTLGMITRVGLRLHAAP
                     PARAFDAYSFPNIEAGVTALRRIYQSGHRPAVARLYDPLDTWLLKDDEPTSTPRGQFA
                     ARRRDLAGRALRLALHAPALLTRSVFGAERCGFGRVALVLMHEGSSAEVESAERAVAA
                     LCREAGGRALGQGPARAWYKHRYHVSYRQSAMFRAGVFVDTMEVSAPWSRIPDVYRSV
                     REAFAPHALIMAHFSHSYPDGSSIYFTFAGTAHGGRSALQVYDDAWRAGLTAALGAGA
                     TLSHHHGIGRSKAARMPEAIGAQLAAQRLLKRALDPNGLFNVGAVLPVPGVSATVTEP
                     SSPAAESELPLWDPHSYLVELPARWTLRQAQEYLAAKSHRLPISMSEISAGGRKLDLD
                     ATLESWVGLGLPGTPDRFSDPNGARLQSFRAQLRNGMKIASSPAPRRAVGPDLSALFI
                     GTRGAFGALETLTLYAPPLLASEPPIMPFLGDRNPPLNAGEQSLLARLERTYAAGGAP
                     RSGPTGDLPG"
     gene            12143..12424
                     /locus_tag="VFQ61_36215"
     CDS             12143..12424
                     /locus_tag="VFQ61_36215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960012.1"
                     /translation="MKYETLDVSGLVDEFVQDFLEIIRTNVESLLRSQMNSARKRLLA
                     KGRAAKSAEASPRRRGRPPGKAGKKAEAQRRGKKSASPRRKPEQLSLFG"
     gene            12536..13258
                     /locus_tag="VFQ61_36220"
     CDS             12536..13258
                     /locus_tag="VFQ61_36220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9960013.1"
                     /translation="MPHEPSSGVVLSARGLWKRYAMGEVVVEALRGVDLDLYAGELVV
                     LLGPSGSGKSTLLNILGGLDVPSQGDVRYQEHVLSEADDAALTRYRREHVGFVFQFYN
                     LIPSLTAKENVELVTDIAAHPLPAEDALSLVGLGDRLEHFPSQLSGGEQQRVAIARAV
                     AKRPELLLCDEPTGALDFKTGQLVLSVLSRINRELGTTTAIITHNAPIALMADRVLTM
                     GDGLLRNERVNEQRRSPEEIVW"
     gene            13252..>14682
                     /locus_tag="VFQ61_36225"
     CDS             13252..>14682
                     /locus_tag="VFQ61_36225"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET9960014.1"
                     /translation="MVRAIERKLLRDLSRLRGQVLTIVLVMACGVGSYVTMQTAYRSL
                     SRAQLAFYEGHRFPDIFAHLKRAPNTLRARLAELPDVRFETRIVEDALLPVPGLSEPA
                     RMAIVSLPDATGAGLARLHLRRGRLPDSQHTDEAVLSEAFGEAHGLEPGARLSIVMGG
                     ARRSLLVVGVALSPEYVFAIGGSGMVQDSRRFGILWLPKAALEPVYAMDASFNDVVVK
                     LSNPKRERATIDALDRLLGPYGGQGAYARDRQVSHMILKSELTQLEQYASVTPAIFLG
                     VSAFLLNLVMSRLLNLQRGQIAALKALGYYDREIVAHYLKLASLVAILGSVLGVLLGA
                     YLGRGMLDLYRPYFRFPNIEYVLEARTVAVAVLICSSFAIGGAWLSVRSVLALSPAEA
                     MLPEAPPNYGRLGLPRWLRYLVGPSERMVLRELLRRPVRTLLSIVGVAFSVAIMVTAR
                     FSFDAVDSMMQMSFETARTEDFEVQFR"
BASE COUNT         2443 a         4664 c         4993 g         2582 t
ORIGIN      
        1 cgtgtagttc gcgtgcgagt tgccgcagca gctgcgggca cgctgggctc gtaatcacct
       61 cgcgcagctc tggcagcgtg caggccgaca gcaacggcac cgaaatcccc tcgggagtac
      121 cgggattgtg gatcagtgcg acacggatcc ggggacggct cagccaccca gtgcgagcga
      181 tgcaggtttg agtgtcgcgg ttggccgggc gacgtgccgc cgcgcgcagc acatcgtctt
      241 cggtgagacg tggattttgg agcagagcct ccatgaccat cggatgcgga tcactcagca
      301 agcgagcgaa agcgctgcga tcggggcgcc gcgcgagcca cttgcgctct ccgagcgtga
      361 gctcacgacc aaccccgtag tccggaatgc gcgaggggcg agcctcgggt tcgacatcgc
      421 tgctggggga acggcgcacc agacgttcga gatggcgcaa gttcaggagt tccgccgctt
      481 ctcgcaggtc atcgacgagc cggctctcgc actggctcgc gagcagcatg gccaagacca
      541 acatgggcgc cttgacggcg ggctcgcgcc gggcgctccg ggccgccatc gcgtccaaca
      601 gcagcgcgac ttcgtcacag gggccctctt gcacgaagcc ctgcagaaac gtgacacgca
      661 gaccgacctc gggaagcgcc gcgagcaacc tgagcagcct ttcgagcttg gcgtggttta
      721 gctccgagga atgcggcgcg ggcgcaccct ccagttcggg atccgaggtc actccttctc
      781 aatgtacccg agcttggtgc ccgtgacagc gctagcccgg ctagacccgc aatccgctga
      841 cttgtcgttt gcggacgctc gggtaggctt gcagccaagt gagcgactcg agccgtgttt
      901 tgcgcttggc agcgctcttt caggagcacc tcgggggggc tccgaggcgc ttttcttggg
      961 ccctgctttt ggtcgcctgg gtaggcgcgg cacatctcgc acgcctcggt acctggacgg
     1021 ggcgcggcgc ggcggtactc gtcttggtga gcagtatcgg gctcgcggtg gctctggttc
     1081 gccgcgaacg ggcccgattg acgggggtcc cggcggtgct gcgccacgtg gtgtggccga
     1141 tcgatccaag actcgggcag cgtgcgtctc gcgcccacga gctgctggtc aagacggaac
     1201 gagaccgcca gtgggggtcg atggagcttg ccgaacatca cttccgcggt ctgctcgcga
     1261 aggtgccgtt gaccgccgtg tcggaggcgg cgcgcgggcg cgcgcggcgt tttctttggg
     1321 gcgcgctggc ctgtttgggc gtgatgaccg ccctgatcgc cgtcgagccc gttcggctac
     1381 tcgagggcta cgatattctc gcggctcggg gcggggtagc gccggtgccg atgccgtggt
     1441 tcgagagcgc ggggatcggg gtaaagcctc ccgcgtatct gcatttgccg gagtacgcgc
     1501 tgttcatggc gagtgatagc gccttgccag agggcagcac ggtcaagatt cgcgggttgc
     1561 cggtgcgacc ggggcgacgc ttggtgttga cggatggtca acaggagctg ccgttcgtgg
     1621 aggatgggtc gggccaagtg gttgcgcagc tgacgcttca gcgctcggta cagcttcgga
     1681 tcgcggcacg cttcggcgat gtgttgatct acgatcctga cgaaatttcg ctgcagatcg
     1741 tgcccgatca gctgcccaaa gtcgaactgg tgggggcccc gcgcgagctc gcgctgcgtg
     1801 aagtggatca cctggaactg ccctactcgg tcagtgacga tcacggcatc aagcagattg
     1861 atttggttct gagatcggcg ggacgagtcg agcggagaac gctctcacgt ttggatgggc
     1921 aaacgttgag cgagcacggc gcgcacgcga tcgatgcgcg agacagcttc ttcaaacggg
     1981 ttttcttgcc ggtccgggtc acgatcgaag cgcgggacaa cgaggcactg cacgacgatc
     2041 gctggagcgc gagtgaagcc atcaccttgc acccgccggg tgtcggtgaa ccgcaagcgc
     2101 tgcgaatgcg tggattgcgc ggcgccgtcg gcgcgttgat tgacgccctc cagagcgagc
     2161 tagcgctcga ggcggtgaag aagggcgatc tcaagtctat tcgtgatgcc gaggcgcgcg
     2221 cgctcgggat gcgcaagcag gcgctcgccg agttggatca ggctttcgat gcaacttatg
     2281 cgtcgctgag cgtcgcgagt gggttgcgcg ctttcttgaa agggcagcta cgaacgctag
     2341 ccgctagccg caccgaccgt aagcgacgtc tagaggacgt agtgctggcg ttggacgcgg
     2401 cgctgcgtgc gcaggcccag cgcgacgccg aggatatctc tagaaggctc ggcgatgtcg
     2461 cggaagaagt agcagaagca gcaaaggcgg cgctcggcag cgaagaacgc aacgcgcgcc
     2521 cgcgcctcga tttggctttg cgagtgctgg atggcggcgc ggagaatctg ctggcgctgg
     2581 gtacgttggg cgcggatctc ggtagcgtgg cgcagggtga actcggccgt attcggcgcg
     2641 ctctgcaagc gcgcagcttg gtcgacaccg agctcgcggc gcgtcatctg gccgcgcgtt
     2701 tgcggcgacc gtccccgtct ttcagttcgg caggcggtgg gtcggtcgag tcaggccggg
     2761 gtagctcgtc ggggccgccg gagggatcgg aggcggaccg tgagttcgat cagatgatgc
     2821 gcgaactcgc ggagctcacg gagcagcacg cggaccaaat tcgcggtctg gagggcatcc
     2881 tggaccaagc cgagcgcggc caaaaagacg accagttgag ccgggaagcc gctgaacgcg
     2941 ccgaacgcat ccgtgaagcg gcgagccaat tgccggaggc cgcggccgcg ctcggtccgg
     3001 cgcgcgccgc gtccgcgcgg gctcgtgagc agctctcgaa catggcgcag aacttcgagc
     3061 gattggatct gaaagaggcg gtggcgcagg ggcgcgccgc ccgcggctcg ctcgacgaag
     3121 cgcttcggtt gcagcaagat ctccagcgcg gcagcgacac ccgagagccg cccgagcaag
     3181 cggctgcgcg cgaagcgctg ggacgtgagc tcgcgtgggc ggaaaagcag ctgcagaagc
     3241 tggccgagca ggccgcgaac cgcgctcgag aggggctggc ggagcgctcg cctcgcgaag
     3301 aaggtttggc gcgccgggcg ggcaacttgg cggggcgcgg ggaacacagc caggccaagt
     3361 tacccgagga gtccgtgcaa gccttggagc aggccgagaa tgcgatgcaa cgcgccgcga
     3421 acgagctcgc gcaaggccgc ggcgaacagg cgctacagct cgcgcgtgag gctcaacgct
     3481 tgatcgagca gagcaatccc gagaacggct ccgatcccca ggatagcgga gaccaacgcg
     3541 ccgaatcgga gcagcgcgcg ggcaaaaaag ggatggcgac caaggcggcg gtgccgggca
     3601 aggacaaggg cgcccgtgcc gaagagtttc ggcgacgcgt gctggaaggc ctctccaaag
     3661 cgcgtcgtga acgcttgtcg aaggccgtcg aacgctatgc ggagggcctc ctcgaatgag
     3721 agccatagct cgtctgagtg cgcacgttgg tctattggcc tctctgctgg ttggcgcgcc
     3781 ttcgagcgcg cgcttgccgg accgcgacgc tcgtctgaag cgattggcag atgccatcac
     3841 cgagatcgac gtggcgcaag cggaacgcct gctctcgggg ctcgattcga acgcgccgga
     3901 agttgcgttc gagtccgcgc gactcgcgat ctaccgcggt gattgcggtc ttgcgcgggt
     3961 gatcctggcg cgccccgacc tggccgagac gcgcgagggg tcggagctca agaattacgc
     4021 ggagggctgc gcgcgggcta ccgctgctgg cttcgtcgtc gaagacgcaa agcgtggcat
     4081 ctggttgcgc gtacaagacg cgcatgatcg cgcgctcgcc ccgttgctgt tcgacgtggc
     4141 ggcccgcgct cgggacgcca tcactcggca ggtcggcgtc gatctaccca agcctctgcg
     4201 catcgacttg gtgcgggatt tgttctccct ctcggcagtt tcgggtctgc cattgagcgc
     4261 tgcggagact accggaactt tggcggtcgc gcgctggggc aaagtgattc tgctctcgcc
     4321 gcgcgcggcc gcacatggtt ttccgtggga agacacgttg gcgcacgaga tcacccatct
     4381 cgtcgtcacg cgcgccacgc gcgaccacgc tccgctctgg ttgcaggaag ggatggccaa
     4441 acggctggag accagctggc gtgagcccca gccgttggac gagccgggct gggccgaagc
     4501 gctggctgtt cgcgcgttca aggagggtcg ctcggtcgga atcgacaaat tgggcgcctc
     4561 gatcgcgatg ttgcctacgc cggaagctgc ctcgattgct ttcgcggagg ttaccagctt
     4621 cgtcgaatac ttcgtgcgtc aggcgggcga gcctgcgctg cggctcttgt tcgccgatct
     4681 gcgcggcgtg cgctctccgg accaagcgct caccagcgta acggggtacc cgctcccgat
     4741 ttggaaccag cgttggcagg ccgcgctggg gcaaattccc gagaccccgg agcgctcacc
     4801 cttcgtggtc gagcatgccc gcgacgtcgc tcggcgcgcg gtgctgagcg accttctgct
     4861 ccagcgcggg catgcggtcg ccgcgctgat ggaggtggaa ccgaccgtca ccgttcctac
     4921 gcggccgggg gcttccgcgc gcgccacggc tgccgctccc caacgcgatc cccagctccg
     4981 gcatcgcgcg gcccgggctg cgttgctggc cggtgatggc gatcgcgccg ccgcactgct
     5041 cgggaccgaa tcggaaattg ccagcgtaca cggaccctgg ttcggggtgc gtgggcgttt
     5101 gcttcggcaa catggggacg gtgtcggtgc ggaacgagcc tggagcctcg gaatcgcggc
     5161 cgatccgttt gctcaggaga ccgcctgtga gggccagtac agccggaccg agtcgccgcc
     5221 cgagacgttg ccgaccgacc caacgcgcca cgagctctgc ttgtccacga ggcagcggcc
     5281 ccgagattga ccggaccgcc cgcgccgcct aggatgcagc ggattacccc ataatattcg
     5341 ctacctcgtg gggcctccct gcgcgccgac cggtgcttcg gtccgtgacg ggaagctgcc
     5401 tcccccacgc ggaaccaatt gtcctgtgtt agtgctcgcg ctcgatcaga ctcaaggcaa
     5461 aaaaatacct ccctcgatac actcttctgg acacacagcc catttaggaa attgtgtgtc
     5521 acgcctccat caaccgaggc gtcttggaag tcgtcgatcg agaacgctcc aaaaggcgag
     5581 gtgcccgagc gcccagaagc tcggagcacc aagagctcga agcctgagcc gccctaaggc
     5641 caacgaggcg agagcagcca gcccagagta aacagccgag agcaaagaag cctgtgagcc
     5701 gctgctcacc gcgagacttc ggaaacccgc aaagaagaaa ccagctcaga aactagctag
     5761 aggtgaaccg gtgagtttgt cgaccttcgc ggcagcccag attttgcgtg cgctgcctcg
     5821 agtccgtatc agtcgtgccg tcggacgttt gtgcgaacgg gaccttccga agcgcgtcgc
     5881 cgatccgttg ctccgagcgt actgtcgtgc gtatcgcgtc aacttggagg aggctgagga
     5941 ggcgtctgcg ggttatccga acttcaacgc cttcttcacc cgtccgttgc gccaaggtgt
     6001 tcgacggttg agtaatgacg cgctggtgag cccgtcggat ggtctgctct cagccgccgg
     6061 ccgcgtggac tccggggctc ggctgttcgt caaaggtcag cactacgagg taggggagct
     6121 gattggggat ctgaacgacg ccgcccgtta ctccggtggg cagttcgcgg tcgtgtatct
     6181 ggcccctggc gattaccatc gcgtgcattc gcccgcggtc gggcgcgtgt cgttggtgcg
     6241 cggcatcgcc ggcgaccttt acccggtcaa tcgcatcggg gaacgccacg tgccgcgctt
     6301 gttcgtgcgc aatagccggg ccgtaatccc aattgacacg gcggagctcg gacgcatcac
     6361 ggtggtaatg gtgggtgctg tgatcgtggg tcgcatcacg gtgaccggct tgaattttcc
     6421 ggatgtgccg cccggtgagc acaccttcga cccggcgcgc gaggtgcagc ccggcgacga
     6481 gatcggcatt tttcatctcg gctcgacagt cgtcgttttg accgagccgg ggttatccat
     6541 cgcacgcccg ctgggtagaa ttcggtatgg cgagtcgttg ctcagggtgg aatgagaaga
     6601 tatcagtggg cagctgacgc atgaccatcg acgagcttca ggacccgacg aaaactgcgg
     6661 cagccgtggg cagcacggct gcggcgccgg tccaagaagc ggtcgcaacg cccgctccag
     6721 aacccaacgc gggttcggag gaagccccgg ctccagctac tggtttgcct gcggtatccc
     6781 gcaaactggc tccgccgccg aagcccaatc aacgcgattc gaagccgagt ttcgcgccaa
     6841 gcggtggtga agagacaccg tcgccgccgg cgcaggttgc ggccccgcgc gcgcccatga
     6901 tcgtcattgg cggcgaccct tcgcctcagc ctgcgcgtgg cgcggcccag gcttggacgc
     6961 gcccagcgtc ggagccacct cccgggcgct ccgagggatc cacgccgccc cctgcgggaa
     7021 ccacgacctc ggcttccgcc gatctgcgtt cgaagctcgg gctttcgcga cccaagcctc
     7081 cgccttctca gcccaacgcg gctcccgcgc ccaatccggc gccgctcgag tcgcgccccg
     7141 cggccgcccg tacgccgccg gcggggcggc tcctggagat gctactcgag gcgaatcacg
     7201 acagtccgct gcggcccaac ctgcctgctg attcctcttc ccgcattccg atctatgacc
     7261 ctgaatcgcg aggcgcggtc agcccgacgg gtgcagcgac gcagccggga ctagctcccg
     7321 tgccccccgc tcccggtgac agctccagta tggaagggcc gccgtcctcg gactggcccg
     7381 atttcgagcc gctgatcgct cgggagcccg ccgggcgtga accggagaag agcggcgtcg
     7441 catcgaacgc ggccgtgagc aagccggggc cggaggctcc actcgttccg ccgccgctgc
     7501 ttggcgggac tgaggtcgtc acgcaaccgg gggcggttac gcaacccatg ctggcgacgc
     7561 cgacgcaggc tgtgccagta gttgccccgc ccgttcccgc gccgccgccg cttgcgagct
     7621 cggccgtagt ggaaccactt gcaccacccg ccacggcgag cggcgcggct tcggcttccg
     7681 agcgtcggat tccagtcgcg accgcaagcg ccgccgcgaa cccgaacgtg gtcctggcgg
     7741 ggagttcggt ctcgatgacg agcgagcctg cgaccagccg cgtctcgaag gaggcggagc
     7801 cgcgcgcaga acgcaacggc catgagcccg ctccggaacc agttccaggt gtggttccaa
     7861 aggcgactcc tgccgctcct ctggcgagca tgaagatgcc cgctctcgcg cccgcggtgg
     7921 ctgctccgag caatcccgcg ccaagcgcct cggcctcggt gcccgcgctc ggaagcaacg
     7981 aggcagcggc accctcgaag cccaaatccg agccagcgcg gctgcgcaat acaccgcgcc
     8041 ctcggctcac ttcggaccct tcactccggg ccgcagtgcc gcctcgtccg gccaaaccca
     8101 gccccgcttc cccatctttt ggagacacgg cggcgtcttc gatcacaggg gattcagcgg
     8161 acgcaggcga gacgagcgat tcgggagaag acgtgccggg acccccgcgc gtaccccgct
     8221 ttgccttgaa cgccagcgcg acgagcacac cgttgccggt gccgtcgggt gggcggcgct
     8281 cggatccgcc ggtacccgca tctgcgatcg cagcgatgaa gatcatcgcg gtgggcgatg
     8341 acgaagcggc cttggcatcc aacctggagc aggtcgtcga gatcgaagag gatgtgccga
     8401 tcccgcccga tttggcggcg gagatcccga tcgacgtcga gcctccccat gaagatgtcg
     8461 aagaggtcgc ggtcgacgag gtagcgccgc aagaggggcg tcagcgtgcg gactcgattc
     8521 cgcccgtcga tgtcgtgatc gaagaggcag aggccgagca gacgctccgt cgcccgggtg
     8581 aggacgtaaa gccaccgcct ccgccgctgc gtcgtccgaa gaccccgagc atcccgcgcg
     8641 cggtgccgca agccaaacgg cggcaaaagc cggcatggtg ggaggaggtc tttcaggaag
     8701 acttcatgcg cgccgccttc cgtctcacgg acgagcagct gcgtgcagaa gtggacttca
     8761 tcgaagagtc cttgggcgtc gcgagtggcg gtgtggtgtt ggatttgggc tgcggcgcgg
     8821 gttatcacgc cgtggaactc gcgagccgcg gctacggggt cgtgggctac gacctctccc
     8881 tttaccagct cgcgctcgcg tcggacgtgg cccaggagcg cggacagaag ctcaatttcc
     8941 tgcagggaga catgcgggag atggcgttcg aagagatgtt cgacggcatc ttctcttgga
     9001 acacgagctt cggttacttc gaagaagaga agaacgtgca cgtcgcggag cgggtattcc
     9061 gcgccctgcg tcctggtgga atgttcctga tcgacgtgct caatcgcgac tatgcggccg
     9121 tgtcttcgcc gtgtcagctc tggtacgaag gcgacgcttg tgtctgcatg gacgacatga
     9181 cgctcgactt catcacgagc cggctgcgcg tcaaacgctc ggtcatcctc gacgatgggc
     9241 gcacgcgcga ggccgtttat tccattcgcc tctactcgct gcacgagctc ggcaagttgc
     9301 tgcacgacgt gggcttccgc gtcaccgagg cgagcggcca ccccgccacg ccaggcgttt
     9361 tcttcggcga gtactcgccg cgcatcatca tgctggctca aaagccgtga ttacttgcag
     9421 gtgacgcggg ccgcagaatc cgcgtcactg cacgagcact ctgcgccgag tgtttgctcc
     9481 aggcagcgac actgattctt cccgttgcaa gccggcagcc acgcgcaagc cgctcccccc
     9541 gcggcgcttt cgtcacaata ccaaccgctc gggcaataac ccttaccaca gctgaagcac
     9601 gtgccgtccg tgcagggggc ctccggcgcg gcatcttcgc ttgcttcgtc ttccgagctt
     9661 gccgatgatt cggcatgccg agctcgagtg tccgaactcg cgatatgctc cgagctcggc
     9721 tcggacttgg cggcaccccc accgcagccg atcgtcagcg cggcgcatgg aaccataccg
     9781 agtgagacga cccaggaacg cgcacgaaga agaagcacga ccgcagatat acgcaagcta
     9841 tcgcgcgtga acaagcctcg taacccgcgc gccgaaagcg ccggtgggca ctcgatggcg
     9901 gcgctcacga gagcggcaga gcgaggcaag cagagcgagc caagcagagt gaggcaaggg
     9961 cggacaacac catgaacctc gatcaagcac tcaaggagct ggcgccgcga gccaaggtga
    10021 gccagagccc ggcggatctt gccgcttatg ccggcgattt gtggccccga gggctggtgc
    10081 aattgcagca ggacacgcgt tcagaacggg caccattggg cgtggtctgg cccgaatccg
    10141 ccgtcgaggt tgctcgaatc ctgcagtttg cacgcgacga agggctcgag atcgtgccgt
    10201 tcggagccgg cagcggcgtt gccggtggca ttctgcccgg tgcgcgtagc ctggtgttgg
    10261 acatgaagcg tctgtccgat ttcggggtcg aagcgggccc caccttgaac gtgggcgccg
    10321 gtgcgttggg catcaccttg gaagagcgtc tgctcgagca gggcttcacc acggggcact
    10381 atccgtccag cattctgtgc tccaccgtcg gtggctgggt cgcggcgcgc ggcatcggtc
    10441 aatgttccgg tcgctacggc aagatcgaag acatggtgac ccggctcgag gtggtgttga
    10501 gtgacgggga ggtggtcgag cttcactcgc gccaaagagg cccggatctg gtgccgttgt
    10561 tcgtcggtag tgagggcact ctcggcatga tcacgcgcgt gggtctacgg ctgcacgcag
    10621 cgcctccggc ccgcgctttc gacgcatact cattcccgaa catcgaggcg ggtgtgaccg
    10681 cgctgcggcg gatctaccaa agcgggcaca gaccagccgt ggcccggctc tatgatccgc
    10741 tcgacacctg gttactgaag gatgacgagc ccacgagcac accccgcggg cagttcgccg
    10801 cgcgcaggcg agacttggca ggccgcgcgt tgcggctggc cttgcacgca ccggcgttgc
    10861 tcacgcgatc cgtatttggc gccgaacgct gcgggttcgg ccgggtcgcg ctggtgctga
    10921 tgcatgaagg gagctcggcc gaagtcgaat ccgcggagcg cgcggtggca gcgctgtgcc
    10981 gcgaggcggg ggggcgtgcg ctcggtcagg gccccgcgcg cgcctggtac aagcaccgtt
    11041 atcacgtgag ctaccgacaa tccgcgatgt ttcgcgcggg cgtgttcgtg gatacgatgg
    11101 aggtttccgc gccgtggtcg cgcattcccg acgtttaccg atcggtacgc gaggctttcg
    11161 ccccgcacgc gctgatcatg gcccatttca gccattccta tcccgacggc agcagcatct
    11221 actttacctt cgcgggtaca gcgcacggcg ggcgaagcgc gttgcaagtg tacgatgacg
    11281 cttggcgcgc ggggctgacg gcggctctcg gcgccggggc aaccctcagc caccaccatg
    11341 gtatcggtcg gagcaaggcg gcccgaatgc cggaagcaat tggggcccag ctcgcggctc
    11401 agcgcttgct caaacgcgcc ctggatccaa acgggctctt caacgtggga gcggttctcc
    11461 cggttccggg tgtctccgca accgtgacgg agccatcgtc gcccgcagcc gagtccgagc
    11521 ttcctctctg ggatccccac agctatctgg tcgagctgcc ggcgcgctgg acgttgaggc
    11581 aagcgcagga gtacttggcc gccaagagtc accgtctgcc gatctcgatg tccgaaatca
    11641 gcgccggcgg tcgaaaactg gatctcgatg cgacactcga gagttgggtc ggtttgggat
    11701 tgcccggcac gccggatcgc ttctcggacc cgaacggcgc ccgtctgcag agcttccgtg
    11761 cccagctgcg taacgggatg aaaatcgcgt cctcaccagc gccacgtcgc gccgtaggcc
    11821 ccgatttgtc cgcgctattc atcggcacgc ggggggcctt cggcgccttg gagacgctca
    11881 cgctttacgc tcccccgctg cttgcgtccg agcctcccat catgcctttc ctgggcgacc
    11941 gtaatcctcc gctgaatgcc ggtgaacaga gcttactcgc tcggctagag cgcacctatg
    12001 cggctggtgg ggcgcctcgc tctgggccca caggcgacct cccgggttga gccaagctat
    12061 gccgacctag aggccagtgt gtgattcggc aggttgcgct tgaccgccgc ggccgtttgt
    12121 cagatttggc catagcgcgc tcatgaaata cgaaaccctg gatgtgagtg ggttggtaga
    12181 cgagttcgtg caggattttc tcgagatcat ccgcacgaac gtcgagtcat tgctgcgtag
    12241 ccaaatgaat tcggcgcgca agcggctcct cgcgaagggg agggctgcca aatccgctga
    12301 agcgagcccg cgccgtcggg gacgcccacc ggggaaagct ggcaagaaag ccgaagccca
    12361 gcggcgcggt aagaagtcag cctcgccgcg ccggaagcca gagcagttgt cactattcgg
    12421 ttgaacccga atattggggg gccagcggag agccgccccg cgcggagcct gatattcggc
    12481 atttgggtaa cggagggagc ttgcatcgcg ctgccgcttt gagaggttga gatccgtgcc
    12541 gcacgagccc agctccggag tcgtgttgtc cgcacgaggg ctctggaaac gctacgcgat
    12601 gggggaagta gtcgtcgagg cgctgcgcgg cgtagacttg gatttgtacg cgggggagct
    12661 cgtggtcttg ctaggtccct cgggtagcgg aaaatccacg ttgctcaaca tcctgggtgg
    12721 cctcgacgtg ccttcgcaag gggatgttcg ctaccaagag cacgttcttt ccgaagccga
    12781 cgacgccgcc ctgacgcgct atcgccgcga gcacgtcgga ttcgtctttc agttctacaa
    12841 tctgatcccg agcctcacgg cgaaagagaa cgtagagctg gtgaccgata tcgcggcgca
    12901 cccgctcccg gccgaagatg ctttgagcct ggtcgggctc ggtgatcgct tggagcactt
    12961 tccgtcgcag ctttccgggg gtgagcaaca gcgcgtcgcg attgcgcgag cggtggccaa
    13021 gcgccccgag ctgttgctgt gcgacgagcc taccggggcg ctcgacttca agacggggca
    13081 gctcgtgctc tcggtgctgt ctcgcatcaa tcgagagctc ggcactacta ccgccatcat
    13141 cacgcacaac gcgccgatcg cgctgatggc ggaccgcgtg ctcaccatgg gtgacggcct
    13201 gctgcgcaat gaacgcgtca acgagcagcg ccgttcgccc gaggagatcg tgtggtgagg
    13261 gcaatcgagc gcaagctgct ccgcgacctg tctcgtttgc gcggccaagt gttgaccatc
    13321 gtgctggtca tggcctgcgg ggtgggcagc tacgtcacga tgcagaccgc atatcgttcg
    13381 ctgtcgcggg cgcagctcgc cttttacgag gggcaccgct tcccggatat tttcgcgcat
    13441 ctcaagcgag cccccaacac gttgcgggct cggctcgccg agctgcccga cgttcgtttc
    13501 gagacccgaa tcgtagaaga tgccttgctg cccgtgcccg ggctctccga gcccgcccgc
    13561 atggcgatcg tgagcctgcc cgacgcgacc ggtgcggggc tggcgcggct ccatttacgg
    13621 cgcggcaggc tgcccgactc acaacacacg gatgaagcgg tgctgagcga agccttcggc
    13681 gaagcgcacg gtttggagcc gggagcgcgc ctttcgatag tgatgggagg agctcggcgt
    13741 tcgctgctcg tggtgggggt ggcgttgtcg cccgagtacg tgttcgcaat cggcggttcg
    13801 ggcatggttc aggacagccg gcgctttggc atcctgtggc tgcccaaggc cgcgctcgag
    13861 cctgtgtacg ccatggatgc gtccttcaat gacgtggtgg tcaagctctc caatccgaag
    13921 cgagagcgtg cgacgatcga tgcgctcgat cgcctgctcg gcccttatgg ggggcagggg
    13981 gcctacgctc gcgatcgcca agtctcgcac atgatcttga agagcgagct cacccagctc
    14041 gagcagtacg ctagcgtgac cccggcgatt tttctgggcg tctctgcgtt cctgctgaat
    14101 ctcgtgatgt cgcgcttgct gaatctccag cgcggacaaa tcgccgcgct gaaggcgctc
    14161 ggctactacg atcgcgagat cgtcgctcac tacttgaagc tcgccagcct ggtcgcgatt
    14221 ttgggctcgg ttttgggcgt tctgctcggg gcctatctgg gccgcggcat gctcgatctg
    14281 tatcgtccgt actttcgctt tccgaacatc gagtacgtct tggaggcgcg caccgtcgcg
    14341 gtggccgtgc tcatctgtag ctccttcgcg attggcggag cctggctgag cgtacgcagt
    14401 gtgctcgcgt tgtctccggc tgaagccatg ctcccggaag caccgccgaa ctacgggcgc
    14461 ctgggcctac cacgctggct gcgttacttg gtcgggcctt ccgaacgcat ggtacttcgg
    14521 gagctcttgc gccgccccgt tcgcacgctg ctcagcatcg tgggggtggc gttctcggtg
    14581 gcgatcatgg tgacggctcg cttcagcttc gacgcggtcg acagcatgat gcagatgagc
    14641 ttcgagaccg cgcggaccga agacttcgag gtacagtttc gc
//
LOCUS       DASTKU010001021         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1987878, whole genome shotgun sequence.
ACCESSION   DASTKU010001021 DASTKU010000000
VERSION     DASTKU010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1987878"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            757..2055
                     /locus_tag="VFQ61_36230"
     CDS             757..2055
                     /locus_tag="VFQ61_36230"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HET9960015.1"
                     /translation="MSHDVRVRSRAQSPASASPTGTSISTVSPATGRSSTFGRAGWHR
                     GLLRLRAWLLAFALLVVGWPAMAASDVRRPADTPQLGDDLGELAPLPRGFDTFDAGWL
                     HLAYPPSLSHWVEPLIQQAHSFRTEASLRLGQSVLSKVRVRLAEDPTQMASLAPTGAP
                     YPKYAVGVAYSKLELILLTQTPVHPNTDHDLLEVFRHELAHLALEEAVQGARVPLWFN
                     EGLAVHLSRENAFARTQTLWTAVVSGNLIPLAEIERHFPDDIVGVPLAYAQSADVVRY
                     LLRTEDQERFRSLIKRLRRGQTFDAAMSDAYGLDSFSLERVWQADAESRYTVWPVLMS
                     GTLVWGGALVLVVLAWRRKRQRQEATLRRWAREEALDELRRLRIAQAAKEPFASPGPQ
                     PSVPPPTQPAVTPEQQGPLPNWGDANVPRVEHEGSWHILH"
     gene            complement(2106..>2426)
                     /locus_tag="VFQ61_36235"
     CDS             complement(2106..>2426)
                     /locus_tag="VFQ61_36235"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET9960016.1"
                     /translation="PLRQGRNPGKVAGVNLPGLSELRQEIDAVDSQILSLLHRRLELV
                     YKVGEIKSVHGVKVYDPERERLMLARLGSMAKPPMAPDTVRRIFERIIDEFRSQEKHH
                     IQEL"
BASE COUNT          407 a          817 c          769 g          433 t
ORIGIN      
        1 cccacgtggg cgcgccgcgc accccaccgc ccccgcgtcg caagtcatcg acgggcacca
       61 cctcgcacgg ccgcgaccga gcggcctcac ggggaggcgg cagcagcctg cggcgaagtc
      121 tccatcgtca gcaaggcggg caggcgaacg cgacaagtgc tgcagtcctc ctgcagcgcc
      181 gcggcctcgg aactaagctc cgcaaatggc gaaagcaagg cgcgcggttc ggtctcctcg
      241 tacttgcttg ttcttcaccc gttaggtgct ctggcaccga cgctcgccac gctctcgccg
      301 aactcggtcg gactcttgca gcgcggggag caactccaag cggacgcgcg tcgagagcgt
      361 gcagcggccg gtcgaaccgt tggtaccgca tgtggtgcaa caggggtcac ggatgcgccc
      421 caaaaaagcg ccgcaactgc gatcgaccga gcgctccccg gtttttcgga ttttaccgcg
      481 agcacccgtg gagacgacgg tgacacgcga cgttggggag gtgatgctca ggcgacgtcg
      541 ccggtccggc gcgacgtcgc gtacttgcgc acccaggggt ttacaccgct ggtgcaacaa
      601 aagggtacaa aagttcggcg ccgcgacgcg ctgcggcgga aaggttgctc cgacatcaag
      661 cacggaaccc catccgccgc atatatcctc atgggttcga gttgacgaac ctcgcctcac
      721 gacccagctt ctagctcagc gtttgcggtg ttgcctatgt ctcacgatgt cagagtgcgt
      781 tcccgagccc agtcccccgc cagcgcttcg cctaccggca cttcgatttc caccgtatcc
      841 cccgcaaccg gccgctcatc gacgtttggc cgtgcggggt ggcaccgcgg actactccgg
      901 ctgcgagcct ggctcttggc gtttgcgctc ctggtcgtgg gctggcccgc gatggcggcc
      961 tccgacgtgc gcagacccgc agatacgccg cagctcggcg atgacctcgg tgagttggcc
     1021 ccgctgccgc ggggcttcga cacgttcgac gcggggtggc tgcacttggc ctacccgccc
     1081 tcgctctctc actgggtgga accgttaatt caacaggcgc actccttccg caccgaggcg
     1141 agcttgcggc tcggacaatc ggtgctcagc aaggtccgcg tgcgtttggc ggaggatccg
     1201 acgcagatgg cgagcctggc gcctactggc gcgccttatc ccaaatacgc ggtgggcgtc
     1261 gcttactcca agctcgagtt gatattgctc acgcagacgc cggtgcaccc caacaccgat
     1321 catgatttgc tcgaggtgtt tcgtcacgag ctcgcacacc tcgcgctcga ggaagccgtc
     1381 cagggcgctc gggtgccgtt gtggttcaac gaaggtctgg cggtacacct ctcacgcgag
     1441 aacgccttcg cacgcactca gactctgtgg acagcggtgg tcagcggcaa cctgatcccg
     1501 ctcgccgaga tcgagcggca cttccccgac gacatcgtgg gtgtgccgct ggcttacgcg
     1561 cagtcggcgg acgtcgtccg ttatctgctg cgaacggagg atcaagagcg cttccgttcg
     1621 ctcatcaaac ggctgcgtcg cggacagacc ttcgacgcgg cgatgtccga cgcctacggc
     1681 ctcgattcgt tcagcctcga acgcgtgtgg caagcggacg ccgagagccg ttacacggtc
     1741 tggcccgttt tgatgagcgg cacgttggtc tggggtggtg cgctcgtgct cgtggtgctg
     1801 gcatggcgcc gcaagcggca acggcaggag gcgaccttac gccgttgggc acgtgaagaa
     1861 gcactcgacg aactgcgacg cttgcggatc gcccaggccg cgaaggagcc gttcgcgtcc
     1921 ccaggtccgc agcccagcgt gccgcccccg acgcaacccg ccgttacccc ggagcagcaa
     1981 ggcccgctgc ccaactgggg cgacgccaac gtaccgcgtg tagaacacga gggcagctgg
     2041 cacatccttc actgatcggt gaaggtggca gccacggagc gacggcattc agcgcccttt
     2101 tgcggtcaaa gctcctggat gtggtgcttt tcttggcttc gaaactcgtc gatgatgcgc
     2161 tcgaagatgc ggcgcacggt gtcgggcgcc atcggcggct tggccatact gcccaatctg
     2221 gcgagcatca ggcgttcgcg ttcggggtcg taaaccttca ctccgtggac gctcttgatc
     2281 tcacccactt tgtagacgag ctccaaacgc cggtgcaaca ggctgaggat ctgcgagtcg
     2341 accgcatcga tttcttggcg cagctctgac aaccccggga gattcacgcc cgcgacctta
     2401 cccggattgc gcccctgccg caacgg
//
LOCUS       DASTKU010001022         2459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1092849, whole genome shotgun sequence.
ACCESSION   DASTKU010001022 DASTKU010000000
VERSION     DASTKU010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092849"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1276
                     /locus_tag="VFQ61_36240"
     CDS             59..1276
                     /locus_tag="VFQ61_36240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960017.1"
                     /translation="MTTLSKWADLFDEIHRDNQRFIRSSFEAWVQNEYLQHGESWQMY
                     GAAVTAGTAEALYTVAGTLGQGLVDLLRVGEGVKRGTALGVAQDALRVVSVVAGGLRV
                     ARLAGSVVKIGGPMSCTLSSSTKAAFLSGNTFRMGALFDRFATLFGGRAAVTAQTFAG
                     TSSPLEMVGLMRILGMGATTRAVKSMAEVTALAAEARGPVIFGVRWATPAGVPGGGHT
                     MVAFRDLLGRIRFADQFGRTVADVAEIGPVASVYHEAAVVLDALVVQALRWGAMGHFL
                     AVPLAPVNPEGMQQLEMAVRKATGRPAPTTASTSPVAPPKPARPAPRPVPATYQVTTT
                     EMCSQRTSEAPIVCQPVTYKSYTVGTNDNLHAISTRVYGTPRYWEVIYRANRGVIGPS
                     PHNLRIGINLYIP"
     gene            complement(1353..>2459)
                     /locus_tag="VFQ61_36245"
     CDS             complement(1353..>2459)
                     /locus_tag="VFQ61_36245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960018.1"
                     /translation="DAPAPNLASRTLELLCVGGASWFLLPLAWALERGLGLDRAELAI
                     GFLMFHGAHVINDPHFAVTYVLFYRGWKRRWSGGELGLLDRVRHVFAGVLVPLLLAGW
                     IGFALVTGSAPALGALIQGMFLLVGWHYVKQGYGVLSVLSARRGRSFSAGERRILLAH
                     CFASWAYAWASPPDAGTILEEKGVVYTSFAHSASLERCAGVVLALSTLALIACLIRKW
                     RSERRTPPLGPLAVFLTTVWLWTIASSIDPLLIYMIPALHSIQYLYFVGLMRWNQARA
                     HEGAPHFGPTARSRLLGLFLGAVALGYVLFHGLPELLDGAIFRPSLRHPRPLTDLGTT
                     PFFAAFFTFVNIHHYFMDNVIWRRSNPETRFLSR"
BASE COUNT          481 a          735 c          821 g          422 t
ORIGIN      
        1 cgaccctagt cgtcggcagc actgctcgct gaaccggagc gcaaactacg gagcttcaat
       61 gacaacgctg agcaaatggg cggacttgtt cgatgagatt caccgagata atcagcggtt
      121 catccgctcg agtttcgaag cctgggtgca gaacgagtat ctccagcacg gtgagagctg
      181 gcagatgtat ggggcagccg tcacggccgg aacggctgag gcgctctaca cggtagcggg
      241 aacactcggt caagggttgg tcgatctgtt gcgagttggg gagggcgtca agcgcggcac
      301 ggcgctcggt gtggcacagg atgcgctgcg ggtagtcagc gtggtggcgg gcgggctgcg
      361 cgtggcgcgc ctcgcgggct cggtcgtgaa gattggcgga ccgatgtcgt gtacgctgtc
      421 ctcatccacc aaagccgcgt tcttgagcgg aaacaccttc cggatgggtg ccttgttcga
      481 ccgcttcgcg acgctgttcg gcggccgtgc tgccgtcacg gctcaaacct tcgcgggtac
      541 gtcgtcgccg ctcgaaatgg tggggttgat gcgtatcctc ggcatgggcg cgaccacgcg
      601 cgcggtcaag tccatggctg aagtgaccgc tttggcggct gaggcgcgcg ggcccgtaat
      661 cttcggtgtg cgctgggcaa ctccagctgg cgtgcccggc gggggccaca cgatggttgc
      721 cttccgcgat ctgctaggtc gcatccgctt cgccgaccaa ttcggtcgca ccgttgcgga
      781 cgtggcggag attgggccgg tagccagtgt ttaccacgag gccgcggtcg ttctcgatgc
      841 cttggtcgtt caagcactcc ggtggggagc gatggggcac ttcctggccg tgccgctggc
      901 accggtgaac ccggaaggta tgcaacagct cgagatggcc gttcgtaagg cgaccggacg
      961 ccccgcgccg accactgcgt cgacctcgcc cgtcgctccg cccaagccag cgcgtccagc
     1021 accgcgtcca gtgcctgcca cgtatcaagt cacgacgacg gagatgtgca gtcagcgcac
     1081 gtcggaagcg ccgatcgtct gccagcccgt tacgtacaag agctacaccg ttggcaccaa
     1141 cgacaacctg cacgcgatct cgaccagggt gtacgggacg ccgagatatt gggaagtcat
     1201 ttatcgggcc aatcgaggtg tcatcggtcc cagcccgcac aatctgcgca ttggtatcaa
     1261 cctctacatt ccgtagttgc tggtggcgcc gagggccgtt gtgtgtatcg agaccacggg
     1321 gggcaacggc cgggctcttg ttgacgaagt gtttagcgag aaagaaagcg ggtctcgggg
     1381 ttggatctgc gccagatgac gttgtccatg aagtaatgat ggatattgac gaacgtgaag
     1441 aaggccgcga agaacggggt ggtgccgaga tcggtgaggg gccgtgggtg ccgcaagctg
     1501 ggccggaata tggcgccatc gagtagctcg ggtaggccgt ggaagagcac gtatccgagc
     1561 gcgacggctc cgaggaagag cccgagcagc cgagaccgcg ccgttgggcc gaaatggggc
     1621 gcgccttcgt gagctcgggc ttgattccat ctcatcaagc cgacgaaata cagatattga
     1681 atcgagtgca gcgccgggat catatagatc agcagtgggt cgatgctgga cgcgatggtc
     1741 cacaaccata ccgtcgtcag aaataccgcg agtgggccga gcggcggtgt tcgcctttca
     1801 cttcgccact tcctgatcag acaggcaatc agcgcgagcg tgctgagcgc gagcaccacg
     1861 cctgcgcacc gctctaggct cgccgagtgc gcaaaactcg tgtagacgac acccttctcc
     1921 tccaggattg tgccggcgtc gggtgggcta gcccaagcgt aggcccaact cgcgaagcag
     1981 tgcgcgagga ggatgcgtct ctccccggcg ctgaacgagc gaccccggcg tgctgagagc
     2041 acgctcaaga ctccatagcc ctgcttcacg tagtgccaac ccacgagcag gaacatgcct
     2101 tgaatcaggg ctccgagagc gggcgctgaa ccggtaacca acgcaaatcc aatccagccc
     2161 gcgagtagca aggggaccag cacccccgcg aagacgtgac ggacgcgatc gagaaggccc
     2221 agctcaccgc ccgaccatct gcgcttccat cctcgataga acagaacgta ggtcacggcg
     2281 aagtgcggat cgttgatcac gtgggcaccg tggaacatca gaaagccaat cgcgagctca
     2341 gcacggtcca gcccgaggcc gcgctcgagc gcccaggcga gcgggagcaa gaaccaactg
     2401 gcgccaccga cacacaacag ttcgagggtg cggctcgcca agttcggggc gggcgcatc
//
LOCUS       DASTKU010001023         7336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1763235, whole genome shotgun sequence.
ACCESSION   DASTKU010001023 DASTKU010000000
VERSION     DASTKU010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763235"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VFQ61_36250"
     CDS             complement(<1..358)
                     /locus_tag="VFQ61_36250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960019.1"
                     /translation="MSRASGSEARLRELLLRARVPPRLPEAVRARALTRARKTVSAPF
                     YVLATPIPPPARITRHALIAAALVLGMGGVAFALNARWKRQSAPAVASPVAGSLPAKA
                     VSSETVPLAHAPTIPSA"
     gene            complement(351..905)
                     /locus_tag="VFQ61_36255"
     CDS             complement(351..905)
                     /locus_tag="VFQ61_36255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9960020.1"
                     /translation="MAYLRLVRSDPPQPAKEHPCVQAFDGDLDYIFETLRRLGARPSE
                     IEDLAHEVFVVLYRNWPSIDTSRSLRPYLFGVAFRIVAAHRRRHARETPYASTDVEDG
                     TADPERDLQSRQSSALLSSALECVPFKRRAVVIMYELDGLSIVEIARTLSISRFGAYA
                     RLHKGRSELANAVRRLQKKRSRDV"
     gene            complement(989..2089)
                     /locus_tag="VFQ61_36260"
     CDS             complement(989..2089)
                     /locus_tag="VFQ61_36260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960021.1"
                     /translation="MGRPLLSGVRRAVLGVWAVVPGVALAFAPAAARAAQVDDALEVS
                     LEYVAPRACPDAIQFQNIVAKRLGYAPFRKQAPSRVLVSIEASEGGVSGHIEWRGPSG
                     EWAGDQTCAARTTECGELARTMAIALAVQIHLLAAMPGASVSGDAAPAPPEAAPAEPA
                     FTAPPAASTTPSPARVDNKGLNSTRLSPSRQSALPRSEAGPQSPWEFGVGGGVSVAHG
                     MSSGLVGFGRLFGVITRRRFSLEFGGEVGVPTVTRRADGAGYSQQLVLASLAGCGVVP
                     PWSGCLLTKAGIARVRGHDIDVPSSDSAPLVQAGVRGGLSSPIGSRLFFALRVEGLAT
                     LTRWTVALDGFGVWTSPPLTMGAGLDLGVHWP"
     gene            complement(2332..3747)
                     /locus_tag="VFQ61_36265"
     CDS             complement(2332..3747)
                     /locus_tag="VFQ61_36265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9960022.1"
                     /translation="MRKFKLSRRAVIKGAGSIAIALPWLEAMRPSRPAYAQNASVPLR
                     RFVTIYQPGGTVRQGSSKGDRYTPTGNETAFQLSPILAPLEPVKSRLLVVDGLDMKCA
                     DQYVFNVEQHQGGSVGLLTGAIQPEAATYPKSPSIDQVLAGRLSNNLPYASLQVAIRW
                     ATGKSHGKISAMNAMYFQASAPYAPIPPRLDPQDIFATLFGSLTGETGTRDSVAALRK
                     KSILDFVDKRYAALSGRLGASDRARLDEHLTKVRELEKRLSSVSAPADAGACKAPARL
                     DTTGYNATSGLNSADNGSLVDSKTDSKIPEVGEFMMDMLVMALACNMTGVASLQWTDT
                     EAKHTFPWLNLNEHHHFYQHDGGFRPDECEKICNWYSQMHLYLLQRMQTVDMGGHSLL
                     DESVVFFGSELSHPPSHAKGNMPFLLAGGDGKTLRTGRWLKYGGQPHNKLLTALLNVF
                     GDSRTTFGDSRLDSTPLTGLA"
     gene            complement(3757..5727)
                     /locus_tag="VFQ61_36270"
     CDS             complement(3757..5727)
                     /locus_tag="VFQ61_36270"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9960023.1"
                     /translation="MRHKNRARAYMDARRDGGRLGIGRSLAGLIVLCSCTAQITDSGD
                     ATALGGASSIQTGGGPAGAGFAGALAGGGAANGSDSSGQNGLGGTQFPVSGNGGAAVS
                     TGGDNAAPPLDCVSPKPGRSPLRRLTTREYNNTVRDLLGDTTKPGDLLPAQVDSKDNW
                     FGNDADFQSVPDTLIEKYQAISEDIAARATGSTSALARLHACAGKAFVPNSPSTSNEE
                     EACARAIAESITPRAYRRAATRLEIDELVALYRSVRALSSTSTFGSGVAAMLEGILQS
                     PDFLYRLEFGTPVAGGTPLRRIAGREMATRLSYLLWQTMPDAALFEAADSGRLDSTEG
                     VVDQARKMLEDSRSHATVAFFFDNFLPIPDLTGLAREASQFPNWSSSVGAAMRGEVQR
                     VIEHEIFENNEATGQHAPGSWPALLTTPYTFANDALFRFYGASTFAPGSVVTGSDFKK
                     VKLNSDQRLGLLTLGGVMAGSTTSNRTNPVLRGIFIVNKVMCSNLELPTGLAPPQIDP
                     YSGKTARERVKKHSQDSFCAGCHRVIDGLGLPFENYDAIGAYRASEKWTDPTSNVTYD
                     TPIDASGAVPGVDGVAANAVELSRQLATSETVQNCFASHWMQFAYGRSLDSSDTCNTW
                     SVRKAFKASGYNVKQLLLSLTQTDAFLYRAAD"
     gene            complement(5868..6860)
                     /locus_tag="VFQ61_36275"
     CDS             complement(5868..6860)
                     /locus_tag="VFQ61_36275"
                     /inference="COORDINATES: protein motif:HMM:NF013806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960024.1"
                     /translation="MPKDFMSSHRLVSVVSLLAVLQACASSSSSGREPTASGGQVASG
                     ASSGGASATQGGAGTSTRPSEGGALGGAQLGGASSGGRETASSMNGGRAGMGSGGSNQ
                     ASGSGGGGASSCINPVFTTSDHNGGFSDGGYYVHNNMWNSEVSLGPETLYACSYRSWY
                     VVSKQTNEQGAVKTYPNVHKDYDDVPIASFERITSSFAATSPHVGIYNVAYDIWTNGV
                     ATSGSTEFMIWTENFNQVPAGDQVASATFGERVYDVWRTSNGHYIALVPEQPFTSGRI
                     DLLEILQWATKRGWLRDDSTLGQIGFGVEIVSTEGKEAVFEFSDFTIQDAGLAP"
     gene            complement(6870..>7336)
                     /locus_tag="VFQ61_36280"
     CDS             complement(6870..>7336)
                     /locus_tag="VFQ61_36280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960025.1"
                     /translation="YLDLVTRLDALSDGAGGTLLDHSLIAWGQESGQLTHWAFSMPVI
                     LAGAAGGSIRTGSYCDYRNLRYRWGGDSGSGNEAKLLWPGLIYNQWLSTILLAMNVPS
                     SEWVDDAHPGFGARVSLPSDFFDYSPGTPKTADVYSNALWQKTGEMLPFLAT"
BASE COUNT         1394 a         2323 c         2232 g         1387 t
ORIGIN      
        1 gtgcagacgg gatcgtaggg gcatgggcga gcgggaccgt ttccgacgaa accgcttttg
       61 ccggtagtga acctgcaacg gggctagcga cagcaggcgc actctggcgt ttccagcgcg
      121 cgttgagcgc gaaggccaca ccgcccatgc ctaaaaccag cgctgccgct atcaaagcat
      181 gacgggtaat gcgcgcgggt ggaggaattg gcgtagccag aacgtaaaag ggcgcgctca
      241 cggtctttcg cgctcgcgtg agcgctcgtg cgcgaaccgc ctccggcagg cgcggcggaa
      301 cgcgcgcgcg aagcaagagc tcccgcaagc gagcttcgga gccgctcgcc ctagacatca
      361 cggctccgct tcttttgaag tcgtcgaact gcattcgcca gctcgcttcg gcctttgtgc
      421 agccgagcgt aggccccgaa ccgactaatg ctgagggtgc gcgcgatctc aacgattgag
      481 agtccgtcga gctcgtacat gatgactacc gctcgccgct tgaagggcac acactcgagc
      541 gccgaggaca acagtgccga cgattgccga gattgcagat ctcgttccgg atccgcagtc
      601 ccgtcttcca catctgtaga tgcataagga gtctcccggg cgtgccggcg ccgatgcgcc
      661 gccacgattc ggaacgctac gccgaacaaa taaggacgca gcgagcgcga ggtgtcgatg
      721 ctcggccaat tgcgatatag caccacgaaa acttcgtggg ccaggtcctc gatctcgctc
      781 gggcgcgccc caaggcgccg taacgtttca aaaatgtagt ccaagtcgcc atcgaacgcc
      841 tggacgcatg gatgttcttt cgccggctgc ggcgggtctg acctaaccag gcgcaagtac
      901 gccattcaac cctgagtact gttgggaagg tggtggttgt tcaaagaatt ctcatccacg
      961 ggtcgcggaa cgaaacagcg gtgaaggttc atggccaatg aactcccagg tccaagcccg
     1021 cccccatcgt caaaggaggc gaggtccaca ccccgaagcc gtcgagggct acggtccagc
     1081 gcgtcagtgt ggcgagccct tcgactcgca gcgcgaagaa cagccggctc ccgatcgggg
     1141 agctgaggcc gccgcgaacc cctgcctgca ccagcggcgc tgagtcagac gaagggacgt
     1201 cgatatcgtg acctctgacc cgcgctatcc ccgccttcgt gaggaggcag ccgctccaag
     1261 gcgggaccac gccgcatccc gcgagactcg cgagcacgag ctgctgcgag tagcccgccc
     1321 cgtctgcacg tcgagtaacg gtcggaactc ccacttctcc gccgaactcg agtgaaaagc
     1381 gccggcgcgt tatcaccccg aagagtcggc caaagcccac gagaccagac gacatgccat
     1441 gggccacgga cacacctccc ccgacgccga attcccaagg tgattgcggc cccgcttccg
     1501 aacgtgggag cgcgctttgc cgactcggac tcagccgagt tgagtttagg cccttgttgt
     1561 ccacccgagc tggcgaaggg gtggtggacg cggcgggggg cgccgtgaat gctggttcag
     1621 cgggtgccgc ttccggtggt gccggagcag catcgcccga tacggacgcg cctggcatgg
     1681 cggcaagaag gtgtatttgc accgcgagcg caatggccat ggtgcgtgcg agctcgccgc
     1741 attcggtcgt tctggcggca caggtttgat ctcctgccca ctctccgctt ggcccacgcc
     1801 actcgatatg gccagatact cccccctcac tcgcctcgat gctaaccagc acgcgcgacg
     1861 gggcctgttt tcgaaacgga gcgtacccta gccgcttggc gacgatattt tgaaactgaa
     1921 ttgcatctgg acaagcgcgt ggagcaacgt actcgagact cacttcgagc gcatcatcca
     1981 cctgcgcggc gcgcgccgca gcaggtgcga aggccagggc gacacctggc acgaccgccc
     2041 acacgcccaa aacggcgcgc ctcacccccg aaagcaacgg tcttcccatc gagcgcgctt
     2101 gctggactct cacgctcgga ccgtgtgtct gcatacctat tcacgcagtg acgtgcgcgg
     2161 ctgacttccg tcaacgcacg accatacaag caggcagcaa acgcgaattc gacgcgaata
     2221 ggtggggctc tcccctacac tcgatccttg atttttctac tgaggctctc gtcgatgatc
     2281 agccctcgtt attacagaac tcgcgacggc cggactgtgc cgagatcgcg ctcatgccaa
     2341 ccctgtcagc ggggtcgagt cgagcctgga atcaccgaag gtcgtgcgac tgtcgccgaa
     2401 aacattcagg agcgctgtga gcagcttatt gtgcggctga ccgccgtact tcagccagcg
     2461 cccggtacga agggtcttgc cgtcaccccc ggcgagcagg aacggcatgt tgcctttggc
     2521 atgggagggc gggtgtgaaa gctcggaacc gaaaaatacc acgctctcgt cgagcagact
     2581 gtgaccgccc atgtccaccg tctgcatgcg ctgcaggaga tagaggtgca tctgtgagta
     2641 ccaattgcag atcttctcgc attcgtcggg gcgaaacccg ccgtcatgct gataaaagtg
     2701 atggtgttcg ttgagattga gccaagggaa cgtgtgcttg gcttccgtat cggtccactg
     2761 tagcgatgca actccggtca tgttgcaggc gagcgccatg accagcatat ccatcatgaa
     2821 ttcgccaacc tcggggatct tcgagtcggt cttcgaatcg acgaggctgc cgttatctgc
     2881 agagttgaga ccgctcgtgg cgttataacc cgtcgtgtcc aatcgcgcgg gcgccttgca
     2941 tgcccccgca tccgcagggg cactcacgct gctcagccgt ttctcgagct cacgcacctt
     3001 ggtgaggtgt tcgtcgagtc gcgctcgatc gctcgccccg agcctgcccg agagcgcggc
     3061 gtaacgcttg tctacgaaat ccaatatcga tttctttcgc agcgcggcga cgctgtctcg
     3121 cgtgccggtt tcccccgtaa gcgagccgaa tagggttgcg aagatatcct gcggatcgag
     3181 ccgcggcgga ataggcgcat agggcgcgct cgcttgaaag tacatggcgt tcatcgccga
     3241 gatcttcccg tgagatttgc cggtcgccca gcgaatcgcc acttgcaagc tggcgtacgg
     3301 aagattgttc gataggcgcc ccgcgagcac ctggtcaatc gagggggact tgggataggt
     3361 cgccgcttca ggttggattg caccagtcag cagccccaca cttcccccct ggtgctgttc
     3421 cacgttgaag acgtattgat ccgcgcattt catgtcgagc ccgtccacca cgagcagacg
     3481 actcttcact ggctcgagcg gagccagaat cggtgaaagt tgaaatgcgg tctcattacc
     3541 cgtcggggta tagcgatcgc ctttcgagct cccctgtcgc acggtgcccc ctggctgata
     3601 gatcgtcacg aagcgccgga gcggtaccga tgcgttttgg gcgtaggccg gacggctcgg
     3661 gcgcatggcc tcgagccacg gcaacgcgat cgcgatactg cccgcaccct taatcacggc
     3721 acggcggctg agcttgaact ttctcatggc acgatttcaa tccgcggcac gatacaaaaa
     3781 ggcatcagtt tgggtcagac tgagcaacag ctgcttcacg ttgtagccag aggctttgaa
     3841 ggctttgcgc accgaccacg tgttgcacgt atcggagctg tcgagggagc gcccgtacgc
     3901 gaactgcatc cagtgggacg caaagcagtt ctggaccgtc tccgatgtcg cgagctgccg
     3961 tgagagttcg acggcgttgg ccgctacccc atcgacgccc gggaccgcgc cactcgcgtc
     4021 gatgggagtg tcgtaagtca cgttcgaagt gggatcggtc cacttctcgg aggcccgata
     4081 cgcacctatc gcgtcgtagt tctcgaacgg cagcccaagc ccatcgatca cgcggtggca
     4141 acccgcacag aaggagtcct gagagtgctt cttgacgcgt tcgcgggcgg tcttcccgct
     4201 ataggggtcg atttgcggtg gtgcaagccc cgtgggcagc tccaggttcg agcacatcac
     4261 cttgttcacg atgaagatac cgcgaagcac tgggttggtc cggttgctcg tcgtggagcc
     4321 tgccatcact ccgccgagtg tgagcaggcc caggcgttgg tcggagttca gcttcacctt
     4381 tttgaagtcg ctgcccgtta ccacgctacc gggcgcgaaa gtggatgccc cgtagaaacg
     4441 gaagagcgca tcgttggcaa atgtgtacgg tgtggtcagg agtgcgggcc agctaccggg
     4501 cgcgtgctgt ccagtcgctt cgttgttctc gaagatctcg tgctcgatga cgcgctgcac
     4561 ctccccccgc atcgccgcgc cgacactgga agaccaattc gggaactgac tcgcctcccg
     4621 cgcgagcccg gtcagatccg gaatcggcag aaagttgtcg aagaagaagg ccaccgtcgc
     4681 gtgagacctc gaatcttcca gcatcttacg cgcttggtcg acaacgccct cggttgaatc
     4741 cagacgtccg gagtccgccg cctcgaagag agccgcatcc ggcatggttt gccacagcaa
     4801 ataagacagc cgcgttgcca tctcgcgacc ggcgatgcgc cgcaacggtg tgcctcccgc
     4861 gacgggcgtg ccaaactcca atcgatagag aaagtccggg gattggagga tcccctcgag
     4921 catcgcggcc acaccggagc caaaagtact ggtggacgaa agcgcacgga ccgaacgata
     4981 gagagctacc agctcgtcga tctcgagtct ggttgccgcg cggcggtaag ctcgaggagt
     5041 gatgctctcg gcgatagcgc gagcgcaggc ttcttcttcg ttactggtcg atggagagtt
     5101 gggtacgaag gctttacctg cgcaggcatg aaggcgcgcc agcgcggagg tactacccgt
     5161 cgctctcgcg gctatatcct ccgagatggc ttggtacttc tcaatcagcg tgtcgggtac
     5221 cgattgaaaa tctgcatcgt tcccgaacca gttatccttg ctgtcgacct gagcgggcag
     5281 caagtcaccg ggtttggtag tgtcccccag caggtcacgc actgtattgt tgtactcgcg
     5341 cgtcgtgagt cgacgcagag gcgaacggcc cggtttgggc gatacgcaat ctagtggcgg
     5401 tgccgcgttg tctcctccgg tcgagacggc ggccccacca ttaccactca ccgggaactg
     5461 cgtgccgccg aggccgttct gtccggagct atccgagccg ttcgcagcac ctccacccgc
     5521 caatgctccg gcgaagccgg caccagccgg gccaccgcca gtctgaatcg acgaggcccc
     5581 gcccagggct gttgcatcgc ctgagtccgt gatttgggct gtgcatgagc agaggacgat
     5641 gagccccgcg aggcttcgtc caatgccgag gcggccaccg tcgcggcggg cgtccatgta
     5701 ggcgcgtgcg cggttcttgt gtcgcatgct gacgtgctcg cagtggatct gcggggacgt
     5761 gctcgaggtt gtgaaccaaa cgacagcgca ctgacgattt atcccgcggg gctcgtttca
     5821 ttctggcccc aaaattctcc ggcccgggcg aaactccgct cgctgagtta cggcgccaaa
     5881 ccagcatcct ggattgtgaa gtcagagaac tcgaataccg cctccttgcc ttcggtcgag
     5941 acgatctcga cgccaaaccc gatctgaccg agcgtcgagt cgtcgcgcaa ccacccgcgc
     6001 ttcgtcgccc attgcaggat ctccaggaga tcgatgcgac ccgacgtgaa cggttgctcg
     6061 ggcaccagcg cgatgtagtg accattcgat gtcctccaga cgtcgtagac gcgctccccg
     6121 aaggtcgcgc tcgcgacttg gtcacccgcg ggaacctggt tgaagttctc cgtccaaatc
     6181 atgaactcag tcgagccgct ggtggcaact ccgttcgtcc agatatcgta agcgacgttg
     6241 taaatgccaa cgtgcgggct cgtcgccgcg aagctgctgg tgattctttc gaacgacgca
     6301 atgggcacat cgtcgtagtc cttgtgcacg ttcgggtacg tcttcaccgc cccttgctcg
     6361 ttggtctgct tggaaaccac gtaccagctt cgataagagc aggcgtacag ggtctcgggc
     6421 cccaagctca cctccgagtt ccacatattg ttgtgcacgt aatagccgcc gtcgctgaat
     6481 cctccgttgt gatcggaggt cgtgaatacc ggattgatgc acgagctcgc accaccgccg
     6541 ccggagccac tggcttggtt cgagcccccc gagcccatac ctgcccgtcc tccgttcatg
     6601 gaggaggcgg tttcgcgtcc accggagctc gcaccgccga gctgtgctcc acctagcgcg
     6661 cccccttcgg atgggcgcgt gctcgtgccc gccccaccct gcgtcgcgct cgcccccccg
     6721 ctcgaagcgc cgctcgcgac ttgcccgcca ctcgccgtgg gctcgcggcc agagctcgac
     6781 gacgaagcgc aggcctggag tacggcgagc agtgagacga ccgaaaccag ccggtgactc
     6841 gacatgaagt cttttggcat gctgcactct caggtagcca ggaacggaag catttcgcca
     6901 gtcttctgcc acagcgcgtt gctgtacacg tcagcagttt ttggcgtacc cggcgagtaa
     6961 tcgaagaagt ccgagggtag agagacacgg gcgccgaatc ccgggtgcgc gtcgtcgacc
     7021 cactcgctcg acggtacgtt catggcgagc agaatcgtgc ttagccattg gttatagatg
     7081 agccccggcc aaagcagctt ggcttcgttc ccgctgccgg agtcacctcc ccagcgatag
     7141 cgcaagttgc gataatcgca gtagctcccc gtacgaatac ttccgcccgc tgcgcccgcc
     7201 aatatcacgg gcatggagaa cgcccaatgc gtgagctggc cggactcctg gccccaggcg
     7261 atgagtgaat ggtcgagtag agttcccccg gccccgtccg agagcgcatc gagtcgagtc
     7321 acgagatcca ggtaga
//
LOCUS       DASTKU010001024         7324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1764419, whole genome shotgun sequence.
ACCESSION   DASTKU010001024 DASTKU010000000
VERSION     DASTKU010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764419"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="VFQ61_36285"
     CDS             <1..1268
                     /locus_tag="VFQ61_36285"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET9960026.1"
                     /translation="TAYLKAHYPTEFFAAALTADKDKIEKVVRTIAEARAWGVSVLPP
                     DINLSETDFTVVYARPEGGRPVKPTGKLRDRLCPQIRFGLGAVRGVGESALETMFESR
                     RASGAFRDLFDFATRVDSKRLNKGVLEALVQCGAFDSVLEGKGISRARAFLAIDRALE
                     RSRSATRDRERGQTTLFGMFDQAKGSTGSSAADEYPTVVEEWDRMELLRREKAALGCY
                     VSGHPLGRYESKLHRLGVVPSQKLADQEPWSMAVIAGMIENYQEKLFKGGSGGRAAFF
                     EVEDTTGRVRAKLRGDRIETYGSLLTSGEPVIVTGKVSFPMTEEPDEEREPTLLVDQV
                     ELLSEAALKATRSVSIRLDAEKTGRKDLVQLKEILEKCPGACPVELVLTLGDGSEAVL
                     DVHGARVTPTDTLLGGLERTFGVSVAELR"
     gene            1336..2586
                     /locus_tag="VFQ61_36290"
     CDS             1336..2586
                     /locus_tag="VFQ61_36290"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9960027.1"
                     /translation="MRDPRAELVRIDSHGQAHPIGSVSSHWLRAREGTYRLLPAPNHV
                     VFMRVAGRDAPSEESTGAVVRLAGEITAPGALCDVVALLGQAAWRGELCVYDGDDIRS
                     VFFSDGNVLGAQTSVLDERIGAILYKFGVIDEAQRDKVLSLQGSGKRFGELVLELGFL
                     NQQELYQFMQRQMEQILYSALTLESGTFFFLQGFDESRLVLRHTVSASSLLMDAVTRM
                     DEMRFFRQKVPSADHVPVRAEGHAAPEEFERVFSVIDSRRSVGEIGRMTGIGEFEATK
                     AVYSLVQNHHVTVHPPAAGPQAVVMAANAALVSIFDAAGQAGQVASLRDSLASFAVMA
                     GVYDILLRDAGPDATGTLDVRKVVENVALIAVGTDPVQVLRQMLHEYVSFAVFCAGAA
                     LGSRFEGELLRNVGPLLAQIRPVG"
     gene            2675..2748
                     /locus_tag="VFQ61_36295"
     tRNA            2675..2748
                     /locus_tag="VFQ61_36295"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2709..2711,aa:Lys,seq:ttt)
     gene            2787..3287
                     /locus_tag="VFQ61_36300"
     CDS             2787..3287
                     /locus_tag="VFQ61_36300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960028.1"
                     /translation="MEQAAWPRFWPTLGALLRKLGQGREKGLELPEAEFGSCPSRAPV
                     PVDHLRRPVSGAIGDPSFRLAVREAQGDEGAPQVVCSQLAGVLTVLVELRAVHPGSLQ
                     VSTQQGSELPGRKGLALLLCEQQGTRFQLAELHVGAQCGGYVGIHCPCPRVVGLVLGE
                     AHRAAF"
     gene            complement(4114..4752)
                     /locus_tag="VFQ61_36305"
     CDS             complement(4114..4752)
                     /locus_tag="VFQ61_36305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960029.1"
                     /translation="MGSDTPREAGAGDRPLGVLAFGILGTLMLAGAYFRFRTGFDVGT
                     LEKHAALGDALAPAGAFLSLLAVFAALWAVQVQRADLALQRQELRETRKEMEEQRKQF
                     ERTARAQESLAAATGDLANRQSEANRLSQFQELAQRRNTLAMLYQVIVTIDLARLQSE
                     FATQPHHEVRALEITEEPREFSLARAKEEGQRIVELESSLGLNTDLAEEEDA"
     gene            complement(5077..5478)
                     /locus_tag="VFQ61_36310"
     CDS             complement(5077..5478)
                     /locus_tag="VFQ61_36310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960030.1"
                     /translation="MTTLRRASIQTVVQGQIVPDLSGLDGPTVLARKREVERQLAATR
                     GDESKLAERERLRAEDAALSQRLTEIKEQTKRDNTRRNFAGIGSPLHEAIVAKLDAAI
                     VAELEADALERLAERERRSAERKARRPGGAP"
     gene            complement(5906..6175)
                     /locus_tag="VFQ61_36315"
     CDS             complement(5906..6175)
                     /locus_tag="VFQ61_36315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960031.1"
                     /translation="MRTVSHPIHRKRVVRIIGRERTPTAASRAAALICALARRSGSDQ
                     LLAIADALHFYWHVLICAARSDAAGIAENRRYVEREIARAFGGAL"
     gene            6602..6922
                     /locus_tag="VFQ61_36320"
     CDS             6602..6922
                     /locus_tag="VFQ61_36320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960032.1"
                     /translation="MSSDDHSETKYEVVDIASLIDAGQSSGEPAVFGAEDLDGYLRRF
                     SDTWGRQHEADATKPASIPVAPRGGTVTLECTIEQARELARRLNYWADQAEAKAKLGN
                     KGGQ"
     gene            6924..7178
                     /locus_tag="VFQ61_36325"
     CDS             6924..7178
                     /locus_tag="VFQ61_36325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960033.1"
                     /translation="MSARDAQHLFEIFESALSELKVLRIGVELLKERSGLSETAFSSA
                     LATLESRKLVSRIGGSLACGETGIEVITEKLVLADVIGER"
BASE COUNT         1296 a         2270 c         2343 g         1415 t
ORIGIN      
        1 aaacggccta tctgaaggcg cactatccga cggagttctt cgccgccgca ttgaccgcgg
       61 acaaggacaa gatcgagaaa gtggtgcgaa cgatcgcgga agcacgggct tggggcgtca
      121 gcgtgctccc gcccgacatc aacctgagcg aaaccgactt cacggtcgtt tatgcgcgcc
      181 cggagggggg ccgtccggtg aaaccgaccg gtaagctgcg cgatcggttg tgtccgcaga
      241 tccgcttcgg actcggcgca gtgcgcgggg ttggtgagtc cgcgctagag accatgttcg
      301 agtcacgccg agcctccggt gcgttccgcg acctgttcga tttcgcgacg cgcgtcgact
      361 ccaagcggct gaacaagggc gtgctcgaag cgctggtaca atgcggagcg ttcgactcgg
      421 tgctcgaagg gaagggaatc agccgagccc gagcgttcct cgccatcgac cgggctctcg
      481 agcgatcgcg cagcgccacg cgcgatcgag aacgcgggca gacgacgttg tttggcatgt
      541 tcgaccaggc caaaggcagc accggtagct cggccgcaga cgagtatcca accgtcgtgg
      601 aagagtggga tcgcatggag ctgctgcgac gtgagaaggc ggcgctcggc tgttatgtat
      661 ccgggcaccc gctcgggcgt tacgagagca agttgcatcg cctcggggtg gtgccgagcc
      721 agaagctcgc cgatcaagag ccgtggtcca tggcggtgat cgccgggatg atcgaaaact
      781 accaggagaa gctgttcaag ggtggctcgg gcgggcgcgc cgcgttcttc gaagtggagg
      841 acacgacggg gcgagttcgc gccaagctgc gcggcgatcg gatcgagacg tacggcagcc
      901 tactgacttc gggtgaaccg gtgatcgtga ccggcaaggt gagcttcccg atgaccgaag
      961 agcctgacga ggagcgcgaa cccacactgc tcgtcgatca ggtagagctg ctttcggaag
     1021 cggcgctcaa agcaacccgc tcggtttcca tccgtttgga cgccgagaag accgggcgaa
     1081 aagacctggt gcagctcaag gaaatcctgg aaaaatgccc aggagcttgc cccgtagagt
     1141 tggtgctcac attgggtgac ggctccgagg cggttctgga cgtacatggc gcccgtgtca
     1201 cgcctaccga caccctgctc ggaggcttgg agcggacttt cggggtgtct gtcgccgagc
     1261 tgcgataggt ccgcgaaatc gcttggccag gacttggccg ttgccgcaat cctccgtaga
     1321 gtactggaat aactcatgcg ggatccgcgt gccgagctcg ttcgcattga ctcgcacggc
     1381 caggctcacc ccatcgggag cgtatcaagt cattggttgc gtgctcggga agggacctat
     1441 cgcctgttgc cagcgcccaa ccacgtggtg ttcatgcgcg tggccgggcg ggacgcgcct
     1501 tcggaggagt ccaccggtgc cgtggtgcga ctcgcgggcg agatcacggc gccaggcgcc
     1561 ttgtgtgacg tcgtggcgtt actcggccaa gccgcgtggc gtggggagct ctgcgtctat
     1621 gacggggatg acatacgcag cgtctttttc tccgatggca acgtgctggg cgcccaaacc
     1681 agcgtgctcg atgagcgcat cggagcgatt ctctacaagt tcggagtgat cgacgaagcg
     1741 cagcgcgaca aagtgctgag cttgcaaggg agcggcaaac gcttcgggga gttggtactc
     1801 gagctcggct tcttgaacca gcaggagctc taccagttca tgcagagaca gatggagcaa
     1861 atcctttact ccgcgctgac gctcgagtcg ggcaccttct tcttcctgca gggcttcgat
     1921 gaatcgcgtt tggtgctgcg ccacacggtc agcgcgagct cactcttgat ggatgccgtc
     1981 acgcgcatgg atgaaatgcg cttctttcgt cagaaggtgc cgagcgcgga tcacgtgccc
     2041 gtccgagccg aggggcacgc cgcgccggaa gagttcgagc gcgtcttcag cgtcatcgat
     2101 tcgcgccgct ccgtcggtga gattggtcgc atgacaggca tcggcgagtt cgaagcgacc
     2161 aaagccgtct actcgctcgt ccagaatcac cacgtgaccg tgcatccgcc tgctgcgggc
     2221 ccgcaagccg tggtcatggc ggccaatgca gcactggtct ccatcttcga cgccgcaggg
     2281 caggccggtc aggtggcatc gctccgcgat agcttggctt cttttgcagt gatggccggc
     2341 gtgtacgaca tcctcctgcg cgatgccggc ccggacgcca ctggcacgct cgacgtgcgc
     2401 aaagtggtcg agaatgtcgc cctcatcgcg gttggtaccg atccggtcca ggtgctgcgg
     2461 cagatgttgc acgaatacgt gagctttgcc gtattctgcg cgggcgccgc gcttggctcg
     2521 cgtttcgagg gcgaactgct gcgcaatgtc ggccccttgc tagctcaaat caggccggtt
     2581 ggctgatgat gccgccctat tgcggcggcc gcgcattttc cgacagtgat ccgttgacct
     2641 cgagcgcgcc gtgtgaaaga catgcgccgc tatcgggtcg atagctcagt tggttagagc
     2701 agcggatttt taatccgttg gtcctgggtt cgagtcccag tcgacccacc gcgattttcc
     2761 tgcgcgttcg tgctacgaga gggcggatgg agcaggcggc gtggcccagg ttctggccca
     2821 ccctcggggc gttgctccgc aaactcgggc agggccgaga gaaggggctc gagctgcccg
     2881 aggccgagtt cggaagctgt ccgagccgcg cgccggtacc ggttgatcat ctgcgacgac
     2941 cggtgtccgg tgcgatcggc gacccaagtt tccgtcttgc cgttcgcgag gctcagggtg
     3001 acgaaggcgc cccgcaggtc gtgtgctctc agcttgcggg tgtgctcacc gtcctcgtgg
     3061 agctccgcgc ggtccacccc ggcagccttc aggtcagcac gcagcagggg agcgagctgc
     3121 caggacgcaa agggctcgcc cttctcctct gtgaacagca aggcaccagg tttcaacttg
     3181 cggagcttca cgtaggcgct caatgcggcg gttacgtggg gatccattgc ccatgcccgc
     3241 gggtcgtcgg tcttgttctc ggtgaggcgc accgcgccgc gttctaggtc gacgtccacc
     3301 cccaggcgta gccccaatgc ttcgcccgag cggcagccct cgcgcgtcag gaagccccac
     3361 agcaagcgcc gcgtgagcgg gacgccgggg cgttctgtgg ctaccccctg ggcttgcgcg
     3421 cgggcatcgc tccagcccag cagggcgcga tcttcagcgg ggtacagata ggcgtagccc
     3481 ggtggccttc caaccttcgg gaggaaccca cggggtaggg ggttcgccac gatgagacga
     3541 cagggaaaga cagcgagcgc gagaacgcgt gcgatgagct gcgcgtaggc tcgccgtgtg
     3601 ctcgggcgcc tcgcggacgc gggcagcttt tgcatgacgg tctggcagtg gtcgagggtc
     3661 accgcggtga gcgggaggtc cccaaacttc agtccggggg cgatcaccac attgcagagc
     3721 gtgtccaggc gcgaagagtc catgtctgcg gtcttcttcg aggaaacgtg atcggggtgg
     3781 tccttcgcga gggtcccgtc cgtccagcgc tccgcgagct gccggaacgt ctccctaggc
     3841 gcctccttcg ccggcctcgt tggccgcgcg cagagctcct gtccaacctt caggtactcc
     3901 gcgaactcgc gttcacttcg agcagccgca gcctccttga gaagcactgc tgcctctgcg
     3961 gagcgacctg cacgcgcgag catctcagcg agctcgcgta gcttcgctcc gcgggatgcg
     4021 gcgagttctg cgtcctgcgt tttgatgagg aaacgcccac gaagaccgtt cccgtagcgt
     4081 aggcgaatgg ggaaaccttc gccctctgat gagttaggca tcctcctcct ctgctaaatc
     4141 ggtgttcaat ccgagcgaac tttccaattc aacgattcgt tgtccctctt ccttagctcg
     4201 tgcgagcgaa aactcgcgcg gctcctctgt aatctccaaa gcccgaacct cgtggtgggg
     4261 ttgtgttgca aactcagact gaaggcgggc taggtctatg gtgacgatca cctgatacag
     4321 cattgctagt gtattcctgc gctgggcgag ctcctggaac tgagacagtc gattcgcttc
     4381 ggactgccgg ttcgcgaggt cgccggtagc ggctgccagg ctctcttgcg ctcttgccgt
     4441 acgctcgaac tgctttcttt gctcctccat ctccttgcga gtctcacgca gctcctgtct
     4501 ctgcagcgcg agatctgcac gctgaacctg aacagcccac agcgcggcga atactgccaa
     4561 gaggctcaag aacgcgcctg cgggcgccag ggcatcaccg agtgctgcat gcttctcgag
     4621 agtccccacg tcaaagccgg ttcgaaacct gaagtacgcc ccggcgagca tcaaggtgcc
     4681 tagaatgccg aatgccaaga cacccaaagg gcgatctccg gcacccgctt ctcgcggtgt
     4741 atcactgccc atgcttcgct accctccgct ttagagttcg atgtgaccgc tttaattctt
     4801 cgacgctggt cgcgagcttg ctgatctctg tagttaggca ctcgagctgc gcaacgtgcg
     4861 aaggcgagcg ttccaacgcc gccttgagtc cgtctatgtc gacccagtac gctcgcgggg
     4921 ctccgcccga ttggaacgtg cgcacgaagg tgccaccaac agcctggttc attgcaagaa
     4981 tgcgccgtcg aattgtcgcg ggatccacgc cctcgcttgc tgcctctcgc gctgagtacg
     5041 cgctcagctt cgtccagcgc gcgtcacgaa gcgctctcac ggcgctcctc cgggccttcg
     5101 cgccttccgc tctgccgagc gccgttcgcg ttccgcaagc ctttcaagcg catcagcctc
     5161 gagctccgca acgattgctg catcgagctt ggcgacgatc gcttcatgca acgggctacc
     5221 gatgccggcg aagttgcgcc gggtgttgtc gcgcttcgtt tgctctttga tctcggtcaa
     5281 ccgttgagag agcgcggcgt cctcggctcg caagcgttcg cgctcagcga gcttggattc
     5341 atctccccgc gttgctgcga gctggcgttc tacctcgcgc ttgcgcgcga gtacggtggg
     5401 cccatcaagg ccggaaagat ccggaacaat ttgcccttga acaacggttt ggatgcttgc
     5461 tcgtcgtaga gtcgtcattg gttcctcaca cgtccccgaa gtcgcacgat gcgcaatgcg
     5521 ggcacagagg gcccgcgaag cggcatagag ccggcagagc cgcctcatgg cgcaccccca
     5581 gctctgccgg tctcgagttc atcacaaacc tcctttcaca agccgaagtg tccgatgggt
     5641 cggcgtcggg aactcgagct ccgctgcgac aaactcgaac caccttcgga ggtggttcgg
     5701 ctgctctagc caactcggat gcaagccccg gatgagtacg gttcgtgctc catcaattgc
     5761 gtcgtcctcc gaaagccaca aagcgcggcg cacgccggga taacgcgttc gagaaagggg
     5821 atgacgttct gcgcccgtcg cgggtttgcg cccgaaacac gagcgctgat ccgaaccgcg
     5881 ccccgcaggc gcgcacgcgt acgcctcaca gtgcaccccc aaacgctcgg gcgatctctc
     5941 gctccacgta gcgccggttc tccgcgatcc cggcggcatc acttcgcgcc gcgcagatca
     6001 ggacgtgcca atagaaatgg agagcgtcag cgatggcaag gagctgatcc gagccggagc
     6061 gccttgccaa ggcgcaaatg agcgccgccg cgcgcgatgc cgcggtcggc gtacgctcgc
     6121 ggccaatgat tctgactact cgtttacgat ggatcgggtg tgatacggtt cgcatgtcgg
     6181 acttcccctg taggttcggc caaggccctc ggccggtgtc agccggcgcg ggggccactc
     6241 ttttaggggc gctcgattac tttttgggct tgggcttctt tggcgagtcg gtgggtcccc
     6301 aacccgcgat cccgtgctcc tgcgcgtact gcctgattac gtatcggagc gcgtctgatc
     6361 gagagctaag accagttcta cgttgcacct ctgcgaggat ctcgaaatcg tccccatcga
     6421 gacggaacgc agtctggcgc aacaccgtag ccatgtatcc caggtgttat caccagggat
     6481 acgctccgtc aagcgtcgat tcgacgtctc ggcgtacgcg cgcttccccc gcagcggcca
     6541 actgagatac gctcaggacg agcggcgcta tcaagcgcgc tccaacacgg aggaggctcg
     6601 ggtgagttct gatgatcaca gcgaaaccaa gtacgaggtg gttgatatcg cttctctgat
     6661 tgatgcaggt cagagctctg gcgagccggc tgtgtttgga gctgaggatc tcgatggcta
     6721 cctgcgccgc ttcagcgaca cctggggtcg ccagcacgaa gccgacgcaa caaagcctgc
     6781 gagcattcct gttgctccgc ggggagggac ggtaacgctc gaatgcacca tcgagcaggc
     6841 gcgagagcta gcaaggcggc tgaactactg ggcggaccag gcggaagcta aagccaagtt
     6901 gggcaacaaa ggtggtcagt agaatgtctg cgcgagatgc acagcaccta ttcgagatct
     6961 tcgaatcggc cctgtctgaa ttgaaggttt tgcgcatcgg cgtggagctt ctcaaggagc
     7021 ggagcggact ctctgagacg gccttctctt cggctctggc gactctggag tcccgcaaat
     7081 tggtcagccg cataggtggg tcacttgcct gcggggagac cggcattgag gtgatcaccg
     7141 agaagcttgt gctcgccgac gtgattgggg aacgttaaag gcctcggaag cagccactct
     7201 ggtcactgta cgcagtacgt gaccccagcg ccctcgcacc gcattccgat tgggcaccaa
     7261 cccgccgacc tcgcatccga gagcagcagc tcttgccgaa gttcaccggc accccgagtt
     7321 cggt
//
LOCUS       DASTKU010001025        17036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1094932, whole genome shotgun sequence.
ACCESSION   DASTKU010001025 DASTKU010000000
VERSION     DASTKU010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 17036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17036
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094932"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1358
                     /locus_tag="VFQ61_36330"
     CDS             162..1358
                     /locus_tag="VFQ61_36330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET9960034.1"
                     /translation="MTSARKFIVLMGVVAMFGDMTYEGARGLVGPYLALLGASATAVG
                     FAAGLGEFLGYGLRLVTGWLGDRTRAYWAFVIVGYATNLVAVPGLAFVGSWQAAVVLL
                     LLERIGKAIRSPARSTLVSYAANQAGVGKSFGIDEALDQLGAVSGPLLTALAIWVARG
                     EAPPRQYQLAFFVLLLPVIANLACLLLAKRRFPDPEAFEPKAQRDPVPISGLFRWYLC
                     AVMLLGLGFADWALIAFHASRTGALSASWVPLVYAGAMGVDALAALGFGALFDRFGLS
                     VLAAAAFISAAFAPLVFSMPAGVSLLLGVLAWSVSMGAQDSVFKATIAKIVPKHERAR
                     AYGIFFALFGLAWWFGSTIMGFLYDRSVGALIAFSVLTQLASVPIFFSLGTRLRAAER
                     EASGAS"
     gene            complement(1416..2576)
                     /locus_tag="VFQ61_36335"
     CDS             complement(1416..2576)
                     /locus_tag="VFQ61_36335"
                     /inference="COORDINATES: protein motif:HMM:NF013806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960035.1"
                     /translation="MTSALAALACSNSASGPGPSASGGAVSSGGVATGGVATGGLATG
                     GAATGGVTATSGGTATGGAPTGGSSGSSSTGGTSTSGGTSASGGTSTTSGGSSNTGGI
                     GGGTAGGGQAGGGATGSGGGTPEATTECKDMASLSGTLTGAMDSQTIEVPVVGKDKSY
                     QLHTNWWHKYTNQAVDYSGLSFTIKDPGNTSVPQTDGAPTGFPTLFIGTYAGHATKGS
                     NLPKQVSAIQSVPTVFSTNALAHDTSNYNATYDVWFTATGDTLPATQFNPGKGGAYLM
                     VWYYDPAQRQPRGGMRNGTTTVANVGAHTVEGVPGTWDVWIDRTDPLCISYVSTTPRD
                     SLEFDLNKFIQDSVKNNYGITASMYLSIVFAGFEIWGGGDGLQTKQFCAKVN"
     gene            complement(3439..4662)
                     /locus_tag="VFQ61_36340"
     CDS             complement(3439..4662)
                     /locus_tag="VFQ61_36340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960036.1"
                     /translation="MLSLRYLLASLAVLGACGEVVHSSDPPQNTGGTKTIAGASTTTN
                     TDAGRGGMTFTPTGGTGGGVQTSTPTGGTTPMPETGGTPGEGGAGIGAAGEPSTLETL
                     CGAEFNFSALSTNDTHVVAVGGRLRLNSDGSCQNEGVIYSSLDGRNWQLRATVPSRVT
                     DVTFGNGVFVAIAPPLDLYVSKDAVTWAPITTGASGLATRLSFGNEVFLAATSSGAPS
                     LLRSKDGRSWEALDRDMPSINQYRSGTRFAAGTFVVWGSGPTVYTTTGGDAVTASKFP
                     VDDQHNPAHSLGTPHVVNDRFVSDTWYNCCFGETGGPWTSYIESKDGFIWKWQGPERA
                     RQYYSIPWVETASLCISIAVTVIHSGPDCAHTSPAMVPPKFTAAAATEWKGVYLAVGS
                     GIAHSTDGKVWERTL"
     gene            complement(4784..7186)
                     /locus_tag="VFQ61_36345"
     CDS             complement(4784..7186)
                     /locus_tag="VFQ61_36345"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9960037.1"
                     /translation="MPGLHVLVVTLDRREQSLQSYPHSASAFDQNDLDRLGLASVRDL
                     GSAIPALEVGNQEGNSEIYIRGVGSNNNTELGDPATATHIDGIYIPRPRGVGSLFFDL
                     ERIESHRGPQGTLRGRNSTAGSLNIVTNKPKLETWEADTSIQFGNYSQRLMRGMLNVP
                     VGKTVALRAAAFSERRDPFYENVTPGSTLRAAEDADSFAYRLGMKWQPTEKIALLVSH
                     DLTREQGVGWAGADFAPALDPERVRGRKDVDPIPAEQIRSPRSVLYRGPQGSMKLTHW
                     GLGANLTADLGAASVEYLSSFRRISYKQASPGSVGIGAVRDVTSDELDNWSTSFFDNT
                     SETIVQELRLFSHDTERLRWTAGIFYFNEQQRAFFATVTDRPAEASFQSEVTVGSEFN
                     MPLMIGDSYATYADATLDLFRDLRVTAGVRLTNEQKRRDGIAYIYTLNRTELRKPGEI
                     RLGTPGFRYEAGSRTDFDPNRAAEDLQSGVAQWGVGDTLQGILEREALDPDSLVKQRE
                     NQYNARFFDYRVGLDYDIRRGSLLYINFSTAHKSGGFNDTLKAGTEVNIPPTYKPEVV
                     YATELGVKNQLLRGRLIVNLATFWYAYTDLQYQVLKNIKNQSYSAARFNAANSRIWGL
                     EGEVVGFLPLNMQLTTSGLLLDARVSSDNSAHIDPRITEQCETPRDCGQLTGKFLPRA
                     PALSVNYALSGSINAKFGIFDWTIAAQSRTKQYMSLLNGEGRDARGNLDYALSDVVPG
                     YTRVNINARWTHPLGYLSLEAFVNNLTDVTYMTSQIQSTKLDLRFFNPPRQFGVRCSL
                     FL"
     gene            7692..8177
                     /locus_tag="VFQ61_36350"
     CDS             7692..8177
                     /locus_tag="VFQ61_36350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960038.1"
                     /translation="MSKRMERLQSLASLGPQRLRVIDCLGGNSPWAPGYWEPPAQGDV
                     FAVYRGYVERMTASFGADLNPVTIRLLAAARLLMGEVAAARVLIEHLPERRIQLDHGA
                     GRCLILPLYVLQTAIPWPSEVGEARSWLAGSAEQRALSSWLDDNAARLVWIEAEGRFL
                     R"
     gene            complement(8212..8886)
                     /locus_tag="VFQ61_36355"
     CDS             complement(8212..8886)
                     /locus_tag="VFQ61_36355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET9960039.1"
                     /translation="MSTLRKPEFQNLIQRLFAEAEASDLRLRQELAQSEAVRAEWQQL
                     AHSDYRAAYARLNHAFLAVSPDTAKLLYMLARARGARNIVEFGTSFGLSTLHLAAAIQ
                     DNGGGVLIGSEFEAEKAAVARANLRAVGLERLVEVREGDALDTLARDLPIAIDFVLLD
                     GAKVLYPKILELLEPNLAPGALVIADNADASPEYLARVRDAGSDYLSVPFGPDVEVSQ
                     YLPHGH"
     gene            8983..9651
                     /locus_tag="VFQ61_36360"
     CDS             8983..9651
                     /locus_tag="VFQ61_36360"
                     /inference="COORDINATES: protein motif:HMM:NF036522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET9960040.1"
                     /translation="MPRRSQPQISARKQPKQARAAGLVADILEAAIRVLKRDGAPGFT
                     TVRVAQEAGVSVGSLYQYFPNKEALLFKLQADEWEDTAALLWEMLADPSIEPLARLRR
                     VVTTFFRSEQQEAELRGALDEAGALFRSSPEARRLQVKTRRHLTEFVRQAVPHASKAE
                     AALIAEVVGNSLWTSAEAVTTQTRARSAIDAYADAMADMYCAYLREFELRCQSRARAA
                     KPRA"
     gene            complement(9697..10644)
                     /locus_tag="VFQ61_36365"
     CDS             complement(9697..10644)
                     /locus_tag="VFQ61_36365"
                     /inference="COORDINATES: protein motif:HMM:NF023304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI immunity family protein"
                     /protein_id="HET9960041.1"
                     /translation="MPSPVVPIEIRDSENGDALVRSGVVAAFFLEPFIDEASEALSTV
                     FDRFVATVPTGELRWAMPSATSEHWKKVDEKLLDRMRSSLTPAASTGKKSRKRRFTAF
                     TINDSGEHAPQYSLTISDRDAAEDGAASRTLLQMTFPLSAAETGSVETLVSLVEETTT
                     RLKPAYGYVSPALLPAEGSRQAAFARMRPITMRHPGYDVPMNELAQLDIGARTRGARW
                     ITLLGPALLQQLGGLDSLRRFASSELELRDLGGTALLRAGKTPEIGDTEQGIPTPGLR
                     SLARVLEPVTLFGEVNLLSYFAAFDEGVLRAWERRFLDP"
     gene            complement(10734..11471)
                     /locus_tag="VFQ61_36370"
     CDS             complement(10734..11471)
                     /locus_tag="VFQ61_36370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960042.1"
                     /translation="MSSTARRFFWPGTISLVLASFAACGGSSDGGLNTSSGTGGASGG
                     LPGASGSFGTMGGRIGGTSTTGSGSTMGSGGARPGGLGGASMTLGGEPGMGPDDGGES
                     GSGGADAGGAPGKPDGPMGPPDGPHPGTDGGMGGMDSGGSAGAGGTSEECPSAPPADK
                     ATCTTLLKCEFTEVTCNCDGPEPDRRWHCTAPPKPMNQCPAMPPKDGAACMTKDNEAA
                     APCHYKEADLDCLCASGKWDCAEVPTP"
     gene            complement(11795..13486)
                     /locus_tag="VFQ61_36375"
     CDS             complement(11795..13486)
                     /locus_tag="VFQ61_36375"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET9960043.1"
                     /translation="MSTLGGNAESAPRTPPLTEREFAEQVEGLARRGQTEELGALLDE
                     SHTVYSERGAAAVVRMRGWVLCVLTRMGRLSSSHLVFLLEELDNGRDPYLVAAAARAL
                     RSQPSPSADWAPYLGKALSNIRYHDDLVTFERYGGYAIGKSGTTALREVLASITWLGA
                     RAQSLAPSLKSLAAQRNGELSSADAEAVDAALRAVDSPPDQHECCSGSLDFLKGLGAF
                     RAWSKETRQRGRLSGVVFEDHLGRRVAYDEVFVGHPSIVVFFYTRCENPEKCSLTVTK
                     LGHLVRALQQRGLGSRIRTAAISYDPAWDSPERLAGYASSRGLTLSEDHRMLRAVEGQ
                     ETLVSGFGLGVSFVESLVSRHRVELYILDTHGRIAAAFERLQWDQDAVVGQACALLDE
                     ATGIRSQLLDQSPVASSMSQNLVPQPTRQRWVSVLTGPGLALATAFFPKCPVCWAAYL
                     SAFGVAGISSIPYSPWMLPVFVVLMLVHLASVRRRAAHQGNHLPLWLSSAGVLSILVF
                     KMALDLPYSGQLGVALSLLGALCTAFMPRQSAAAARPIMAHGESDRGTQDLGSCQ"
     gene            complement(13493..14458)
                     /locus_tag="VFQ61_36380"
     CDS             complement(13493..14458)
                     /locus_tag="VFQ61_36380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960044.1"
                     /translation="MRAKKSKARKSGDAQRKAEPQKAEPRKAKQSRARREEASAERAR
                     GAVSSKAALSDAQDRRKSVRVRAAGLEKLAGAVCAEDKTFNAVLAILQDPDEPPELRS
                     TALRTLQAASFSVVAFNPRRAEYLATLREMSSDAEPELRQSVLGILVRERDGYAQQLL
                     LEGLQDPKAALVSPEKALQLLSYDVHTEAYPMARKIARKPPTPEAKREALRLLGADAG
                     SVALFESVLRNKNEPAEFRRLSAAALQALKPAALQTHAREIVLDDDEDEEVKAASLTA
                     LSLFGAADEMGRDDELRAKTTDLKSSGSPELKRGAKAFLARYHRV"
     gene            complement(14484..15701)
                     /locus_tag="VFQ61_36385"
     CDS             complement(14484..15701)
                     /locus_tag="VFQ61_36385"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HET9960045.1"
                     /translation="MAIELSINNTASARGRYVTWAPSPCILRATPPFTPPLPATLRVR
                     NRPGTGIRVAFRRTATSAPQSSLTLTLSPGAAAVSFFVSGVFPRASTADGDVAIQVLS
                     GAAVLLDVRLMVRIRKNANRLTPQERDRFLAALAALNNRGAGRFLDFRNMHTEAGSPE
                     AHGAPGFLPWHRAYLLDLERELQAIDSSVSLPYWRFDQPAPNLFTPEFMGTSDRLGTV
                     GFSAMNPLQFWRTDGVPGINRRPRFDTRSQAALVINEAATLALGTNYSAFRSMEGNPH
                     GFAHTSFGGFISSIPTAARDPLFFLLHANVDRLWAKWQRTNNRFNPGQLAAFAPATGS
                     RVGHNVDDTMWPWNGVTTAPRPSTAPGGALAPSPTAVAPGGSPRVRDCLDYQGRLASA
                     SRMGFDYDDVNYL"
     gene            complement(15877..>17036)
                     /locus_tag="VFQ61_36390"
     CDS             complement(15877..>17036)
                     /locus_tag="VFQ61_36390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9960046.1"
                     /translation="LTGGIAHDFNNILAAVMANVALASEDVAPNHPVQQALSEIRQAS
                     LRAAALVRQILTFSRRGVPERRWICLHDIVGEALKLLRASLPASIRIEEHFAADATEI
                     FADPTQIHQVIMNLGTNAAHAMQDFNRAPHAGILTVSIKRFEIGPGFMAGGNELKPGL
                     YVQLSFADNGSGMDSETLAKIFDPFFTTKEPGTGTGLGLSVVHGVVRSHDAGMVVLSK
                     PGQGTEFNIYFPARDARAVIGDDKIASNCMPGNGEQLLCIDDELPVLRATSRLLERLG
                     YLPVSFSDPRQALAALANTPERFAAVVSDCSMPDIWGLELVRELRRIAPNIPVVMTSG
                     LLDPALVKDLRTLGVREFLAKPGTLEELSCALHRALHGAERESAPVPNATL"
BASE COUNT         3113 a         5314 c         5347 g         3262 t
ORIGIN      
        1 agacccggtc gtcgggagag ccgggcgcgg gacgggacgc ttcgaacggg ggcggggcat
       61 gggcatcgaa acgaggacgt cgcagcttgc aacaggccgg gggagcgcga aagcactaac
      121 cgaactcttg ggccatactc cggacctgga gagcgaactc aatgacctcc gcccgcaaat
      181 tcatcgtgct catgggcgtg gtcgccatgt ttggagacat gacctacgag ggcgcgcgcg
      241 ggctcgtagg gccctatctc gccctgctcg gggctagtgc gaccgcggtc ggtttcgcgg
      301 cgggtctcgg cgaatttctg ggctacgggc tgcgactcgt cacgggctgg ctaggcgatc
      361 gcacgcgagc ctactgggcg ttcgtcatcg tgggttacgc cacgaacctg gtggctgtgc
      421 cgggcctcgc gttcgtaggc agctggcaag ctgccgtcgt actcctgctg ctcgaacgga
      481 tcggcaaagc cattcgcagc ccggctcgca gcacactcgt gtcctacgcg gccaatcaag
      541 ccggcgtcgg caagagcttc ggcatcgacg aggccctcga tcagcttggc gccgtgagcg
      601 gcccgctcct gaccgcgctc gcgatttggg ttgcgcgcgg cgaggcgccc cctcggcagt
      661 accaactcgc attcttcgtt ttgctcctgc ccgtcatcgc caatctggca tgcctgctgc
      721 ttgccaaacg gcgcttcccc gatccggagg cattcgaacc caaggcgcag cgcgaccccg
      781 tgcccatcag cgggctcttc cggtggtatc tgtgcgcggt gatgctgctc gggctcggct
      841 ttgccgactg ggccctcatc gcctttcacg ccagccgcac cggcgcgctc agtgcgagct
      901 gggttccgct cgtttatgcc ggcgcgatgg gcgtcgacgc gctcgcggcg ctcggctttg
      961 gtgccctctt cgaccgcttc ggtctgtccg tgctcgcggc cgccgccttt atctccgcgg
     1021 cgttcgcgcc gctcgtattt tcaatgcccg ccggggtaag cctattgcta ggcgtgctcg
     1081 cgtggtcggt gagcatgggc gctcaggact cggtcttcaa agccaccatc gccaaaatcg
     1141 tgcctaaaca cgagcgcgcc cgcgcctacg gcatcttctt cgcgctattc gggctcgcct
     1201 ggtggttcgg gagcaccatc atgggcttcc tctacgaccg ctccgtcggc gcgctcattg
     1261 ccttctccgt cctcacgcag ctcgcgtccg tgcccatctt ctttagtttg ggaacacggt
     1321 tgcgagccgc ggagagggaa gcttctggcg ccagttgaag aagcgggaat ccgcctggtc
     1381 gatctagaac gacgggaaat cgacgaggag gggagtcaat tgaccttggc gcagaactgc
     1441 ttggtttgca gtccgtcgcc gccgccccaa atctcgaaac cggcgaatac gatactcagg
     1501 tacatactgg cggtgatccc gtagttgttc ttcaccgagt cctgaatgaa cttgttcaga
     1561 tcgaactcca gagaatcacg cggcgtcgta ctcacgtacg agatgcacag cggatccgtg
     1621 cgatcgatcc aaacgtccca cgtgcccgga acgccttcga ccgtgtgcgc tcccacgttg
     1681 gccaccgtcg tcgtcccgtt gcgcatgcca cctcgcggct gtcgctgcgc cggatcgtaa
     1741 taccacacca tcaggtacgc cccgccctta ccgggattga attgcgtggc cggcagcgta
     1801 tcgcccgtcg cggtgaacca cacgtcgtaa gtcgcgttgt agttgctcgt atcgtgggcc
     1861 agagcattcg tcgaaaacac cgtcggcacc gactggattg ccgacacctg cttcggcaga
     1921 ttgctgccct tcgtcgcgtg gcccgcgtaa gtcccgatga acaacgttgg gaaccccgtc
     1981 ggcgcgccgt ccgtctgcgg tacggacgta ttccctgggt ctttgatcgt gaacgagagc
     2041 ccgctgtaat ccaccgcttg gttcgtgtac ttgtgccacc aattcgtatg cagttggtaa
     2101 ctcttgtcct taccgacgac cggtacctcg atcgtttgcg aatccatcgc tcccgtcagc
     2161 gtaccgctga gtgaagccat gtctttgcac tcggtcgtag cctctggcgt accgccgccg
     2221 ctgcccgtcg ccccaccgcc ggcttgacca ccgcccgcgg tgccgccgcc gattccgccc
     2281 gtattcgagc ttccgccact cgtcgtgctc gttccacccg aggcgctcgt accaccgctc
     2341 gtcgaggtgc cgcccgtgct cgagctcccc gaacttccac cggttggcgc gccgcccgtt
     2401 gcggtcccgc cgctcgtcgc ggtcacccct cccgtagccg caccaccagt cgccagtcct
     2461 cccgtagcca ctccgccagt cgccactccg ccactagaga cggcgccacc cgaagccgaa
     2521 ggccccgggc ccgatgcgga attggaacaa gccaaagcag cgagagcaga agtcacgcca
     2581 aaccaaaaag aaacacgaag cgagcgcata acacctcagt caatgcacaa ggacgcgagc
     2641 aggccgagag tctatccgaa ctcccccgct cttcaattca cccgtgctcc tggggccact
     2701 ttgctcatgt cagggctcca ggatcactca actattcgtc cgtggtcgcc acctaattgc
     2761 aaaaccgctc acgttgaccc gcgtattgaa tctctgtcag tcatccatcc aaaccgcggg
     2821 ggcctgcgcg ttgcgacgtg cacgctcgag ctcccgcgcc tgagcctttg cgacccccac
     2881 cgcgcctcgc gctcgtcccc taggcggccc caaccaacct cgccagacca ccgccatctt
     2941 cgatgggctc gtgcttaccg gcgcaatggc caacctgcac gacatcgacg agcgcggcga
     3001 atgttttggc cacgagacaa cgagcgcgag gcgcggcgtt tgacgtcgca agcgcagccg
     3061 ggtggcgcgg gctaactcca atgcaccgcg caggccctcg gggcagacgc tcgcaagatg
     3121 gccgattcaa gatcgacgtc tgactgcagc gcgatctgcg tacgatgcaa ctctccagtg
     3181 cttgccgtgc acgccggcag aggagcggta gcgaagggcc gagcgcgata ctccgcaaaa
     3241 cggtctggga tacgattcgt tcgtctcggg agcagttgag tctgaatttc ccgaacacgg
     3301 gtttccagaa taaatccggc ggtaaacgcg gtatcgggtg tgcgaagcgg ctaaatgacg
     3361 gcgataatag gggttgaggg gcggcgctcg cgcggtgtgg atgccgcagt caaccgcgac
     3421 agcggtcgct ccgttcagtc atagcgttcg ctcccacacc ttcccgtccg tgctgtgcgc
     3481 gattccgcta ccaaccgcga ggtagacacc cttccactcg gtggcggctg cggccgtgaa
     3541 tttcggtggc accatcgcgg gggaggtgtg ggcgcagtcg gggccgctat gtatgaccgt
     3601 gaccgcaatc gaaatgcaga gcgaagctgt ctcgacccac ggaatcgaat aatactgacg
     3661 agcacgctcg ggtccctgcc acttccaaat gaatccgtct ttggattcga tatagctggt
     3721 ccagggtccg ccagtctcac cgaagcagca gttgtaccaa gtgtcgctca cgaaacggtc
     3781 gttgacgacg tgcggtgttc ccaaactgtg agctgggttg tgctggtcat ccacggggaa
     3841 cttgctggcg gtgaccgcgt ctccccctgt ggtcgtgtag acggtcgggc ccgagcccca
     3901 aaccacgaat gtcccggccg cgaagcgggt gccacttcgg tattggttga tactgggcat
     3961 gtcccgatcg agggcttccc agctgcgacc atctttggag cgcaggagag agggcgcgcc
     4021 gctggacgtg gccgcgagaa agacttcgtt tccaaacgat aggcgggtgg ctaaccccga
     4081 ggcgcccgtt gtgatggggg cccacgtgac cgcgtctttg gaaacataga gatcgagcgg
     4141 gggtgcgatc gcgacgaaca caccattgcc gaaggtgacg tcggtgactc tggacggcac
     4201 cgttgcacga agctgccaat tccgtccatc gagcgagctg tagatgactc cttcgttctg
     4261 gcaactcccg tccgagttca ggcggagccg cccgccgacg gcaacaacgt gggtgtcgtt
     4321 cgtgctgagc gcggaaaagt tgaattcggc tccacacaga gtttcgaggg tactcggctc
     4381 gcccgctgct ccgatgccag cgccgccctc cccgggagtt ccgcccgttt cgggcattgg
     4441 cgtggttccc ccggtcgggg tggaggtttg cacgcctcca ccggtgccgc cggttggcgt
     4501 gaaggtcatt ccgcctcttc ccgcgtccgt gttcgtggtc gtgctcgcgc ccgcgatggt
     4561 cttggttccg ccggtgttct gaggtgggtc ggaactgtgc acgacctcac cacaagcacc
     4621 gagcaccgct aggctggcca agaggtatcg taggctgagc atgcatccgg catcgcaacc
     4681 tccgtgccag gcgaaccgag ctaattctcg gaggctctgg gctctgcgcc gggccgaagc
     4741 gtgccggagt gtgccaccgc ttcgtcagtg gctcgagccg gcttcaaagg aagagtgagc
     4801 agcgcacgcc gaactgccgg ggagggttga agaagcgcag atcgagcttg gtggattgaa
     4861 tctggctggt catgtacgtc acgtcggtga ggttgttcac gaaggcctct aggctcaaat
     4921 agccgagcgg gtgcgtccag cgggcgttga tgttgactcg ggtgtacccg ggcaccacgt
     4981 cgctcagagc gtagtcgaga ttgccccgcg cgtcgcgacc ttcaccgttc aacaaggaca
     5041 tgtactgctt cgttcggctc tgggcggcta tcgtccaatc gaatattcca aactttgcgt
     5101 tgatgctgcc gctcagcgcg tagttgacgc tcagggcggg agcccgtggc aagaacttac
     5161 ccgtgagctg cccgcaatct cgaggggttt cgcactgctc ggtgatgcga ggatcgatat
     5221 gcgccgagtt gtccgaggag acgcgcgcgt ccagcagtag gccggaggtc gtcagttgca
     5281 tgttgagcgg caagaatcca acgacctctc cttccaatcc ccagatgcgt gagtttgcgg
     5341 cgttgaagcg agctgcggaa taggattggt ttttgatgtt cttcaacact tgatattgaa
     5401 gatcggtgta tgcataccaa aacgtcgcca agttgacgat caagcgcccg cggaggagct
     5461 ggtttttgac ccccagctcg gtcgcataga cgacctctgg cttgtacgtc ggagggatgt
     5521 tgacctcggt gccggctttg agggtatcgt tgaagccacc cgatttgtga gccgtggaaa
     5581 agttgatata aagcaggctg ccccgtcgaa tgtcgtagtc gagcccgact cgataatcga
     5641 aaaacctggc attgtattgg ttttcgcgct gcttgacgag cgagtcggga tccagggctt
     5701 ctcgctcgag aatgccttgt agcgtgtccc caacgcccca ttgggctact cccgactgca
     5761 ggtcttccgc agcgcggttg gggtcgaagt cagtgcgtga accagcctca taacgaaacc
     5821 ctggggttcc aaggcgaatt tctccgggct tacgcaactc tgttctattc agggtgtaga
     5881 tgtaggcgat tccgtcgcgg cgcttctgct cgttcgtgag gcgcactcca gccgtgaccc
     5941 gcaaatcgcg aaatagatcg agggttgcgt ccgcgtaggt agcgtacgag tcgccgatca
     6001 tcaaaggcat attgaattca ctgccgaccg tcacctcgct ttgaaaactc gcctccgcgg
     6061 ggcggtcggt caccgtcgcg aaaaaggcac gctgttgctc attgaagtag aatattccgg
     6121 ctgtccagcg taggcgttcg gtgtcgtgac tgaagaggcg gagctcttgc acgatggtct
     6181 ccgaggtgtt gtcgaagaat gacgtgctcc agttatcgag ctcgtcactg gtgacgtcgc
     6241 gaaccgcgcc gattccgacg ctgccggggc tggcttgttt ataggaaatg cgcctgaagc
     6301 tcgagagata ctcgaccgat gcagcaccca agtccgccgt gaggttggcg ccgagtcccc
     6361 agtgcgtgag cttcatgctg ccctgaggtc cccggtaaag cacggagcgg ggggaacgga
     6421 tctgctcggc cgggatggga tccacgtctt tgcgacccct aactcgttcg gggtcgaggg
     6481 ctggggcgaa gtctgcacca gcccagccca cgccctgttc tcgcgttaga tcgtggctca
     6541 ccaggagcgc gatcttctcg gtgggctgcc atttcatgcc gaggcgatat gcgaaggaat
     6601 cggcatcttc tgccgcgcgc agggtgctgc cgggggtcac gttctcgtag aacgggtcgc
     6661 gccgctccga aaacgcggcc gcgcgcaggg cgacggtttt acccacggga acgttcagca
     6721 tgccgcgcat cagtcgttgc gagtagttgc caaattggat cgatgtgtct gcctcccagg
     6781 tttccagctt gggcttgttg gtgactatgt tcaaagagcc cgcggtcgag ttgcggccgc
     6841 gaagtgtgcc ctgcggtccc cgatgggatt cgattcgctc caaatcgaaa aacaacgacc
     6901 cgacaccgcg ggggcgtggg atatagatgc cgtcgatatg ggtggcagtc gccgggtctc
     6961 cgagctcggt gttgttgttc gagcccacac cgcgaatgta tatttcagaa ttgccttctt
     7021 gattgcctac ctccagcgcg ggtatcgcgc tgcccaagtc gcgcaccgag gccagcccga
     7081 gccgatcgag gtcgttttga tcgaaggcgc tcgcggaatg tgggtagctt tgcaaggatt
     7141 gctcccgccg atcgagcgtc accacgagca catgcaaacc aggcacgtga tagacgtgct
     7201 ctttgggtgt aggctcggcg ctcgaggacg gggccggtgg ggccggcgcg ctttggtcct
     7261 cgacgtttga agggtcgggc gagacactgg aggagccggt cgaatctccg cctgcgggca
     7321 agcgagagtt tcccggggtc ggccctcgcg agggctcaga cgctagttgg tggactggcg
     7381 cgtcgtgagg ggaacggggc tccgaggaag ttttcgccag taatggatgt gtccaaagcg
     7441 agagcgccac ggtggccgcc aaggaccaaa catcgctacc gagcgctcgt gtgcaacgca
     7501 tggactgccg ccaatggact gatgtccgct cgcgcacaaa ccgtgctccg aaagtaggca
     7561 atgcacgaca gattcaggcg ccccggctgg gcgttgtgaa tggggcgttc cacgcggggt
     7621 cgcttatcga agcgcgtcgt cgaacaaact cccaatggct ccggtgctgg cgcaacctcg
     7681 acgtgtactt catgagcaaa cggatggagc gactccagtc cctcgcgtca ctcggacccc
     7741 aacggctccg ggtaatcgat tgcctgggcg gcaattcacc ctgggcacca ggctattggg
     7801 agcctccggc ccagggtgac gtgtttgcgg tgtaccgcgg gtacgtcgag cgcatgactg
     7861 cttccttcgg cgcagacctg aaccccgtca cgatccggct gctcgccgcc gcgcgcttgc
     7921 tcatgggaga agtcgcggcg gcgagggtgc tgattgagca tctgcccgag cggcgcatcc
     7981 aactcgatca tggcgcggga cgttgcctca tcctgccgct ctacgtgctc caaaccgcga
     8041 tcccgtggcc aagcgaggtg ggggaggcgc gcagctggtt ggcgggctcg gcagaacagc
     8101 gcgcgctgag cagctggtta gacgacaacg cagctcgctt ggtctggatc gaggccgagg
     8161 gccggttctt gcgctgactt tcggcttgcc tcgaggcgga gatacgcttc ttcagtgtcc
     8221 gtggggtaag tactgcgaga cctcgacgtc tggcccgaac ggtacagaca ggtagtcaga
     8281 gcccgcgtcg cgaacccgcg ccaagtactc ggggcttgcg tcggcattat cggcaatcac
     8341 gagcgcgccg ggcgcgaggt taggctccaa tagctcgagg atcttcggat agagcacctt
     8401 ggcgccgtcc agcaaaacga agtcgatggc tatcggcaag tcgcgcgcca gagtgtcgag
     8461 cgcgtcgcct tcgcgaacct cgaccagtcg ttcgagcccc acggcacgca gattggctcg
     8521 tgccaccgcg gctttctcgg cttcgaactc actgccgatc aacacgcctc ccccattgtc
     8581 ctgaatggcg gccgccaaat gcagtgtcga gagcccaaat gacgtgccga actcgacgat
     8641 gtttcgcgcg cctcgagctc gagccagcat gtagagcagc ttggcggtat cgggtgaaac
     8701 cgccaagaag gcatggttca agcgagcata ggcggcgcga tagtcggagt gagcgagctg
     8761 ctgccattcg gcccggaccg cctccgactg cgcgagttcc tgtcgtaagc gcaggtcgct
     8821 cgcctcggct tcggcgaaca ggcgttgaat caaattctga aattctggtt ttcggagcgt
     8881 agacatacga agcctccagc ggtgttgcag ctagcgccct gctgagcgct ccgatagaat
     8941 acgcgccgcc cttgctacgg gaattcgcgt tcccgattga acatgccgag acgatcgcaa
     9001 ccccagattt ccgcgagaaa acagccgaag caggcccgcg cggcggggct cgtggcagac
     9061 atcttggaag cggccattcg cgttttgaag cgtgatggcg ccccggggtt tacgactgtc
     9121 cgggtggcgc aagaagcggg cgtgagcgtc ggctcgctat atcagtactt ccccaacaag
     9181 gaagcgctgt tgttcaagct gcaagccgac gaatgggaag acaccgccgc gctgctctgg
     9241 gagatgctcg cggacccaag catcgaaccg ctggcgcgcc tacggcgagt ggtgacgacg
     9301 ttctttcggt ccgaacaaca agaggcagaa ttgcgcggtg cgctggacga ggctggcgcg
     9361 ctgtttcgga gtagccccga agcgcgccgc ttgcaggtga agactaggcg tcatctaacc
     9421 gagttcgtgc gccaagccgt gccccacgcc agcaaggccg aagcggcttt gatcgcggag
     9481 gtcgtgggga atagtctgtg gacctcggcc gaagcagtca ctacgcaaac gcgagcccga
     9541 tctgcgatcg acgcttatgc ggatgcgatg gcggatatgt attgtgctta tctccgcgag
     9601 ttcgagctcc gctgtcagag cagagctcgg gccgcgaaac cgcgcgcgta aaggttgcgc
     9661 gcgcacgaga aaagccgggt gatgatactc cgcgcgtcag gggtccaaaa atcgccgctc
     9721 ccacgcccgc aggacgccct cgtcgaacgc ggcaaagtag gagagcaagt tgacctctcc
     9781 gaatagcgtg acgggctcga gcacgcgcgc gaggctgcgc agacccggag ttggaatgcc
     9841 ttgctcggtg tcaccaattt cgggtgtctt cccggcgcgg agaagggcgg tgccgccaag
     9901 atcccgaagc tcgagttcac tggacgcaaa acgccgcaac gaatcgagcc cgccgagctg
     9961 ctgcagaagc gcaggaccga gcagcgtgat ccatcgggcc ccgcgcgtac gagcgccgat
    10021 gtcgagctgc gcgagctcat tcatgggcac gtcatagccg gggtgccgca tcgtaatcgg
    10081 gcgcatccgg gcgaatgccg cttgccgcga accttcggcg ggaagcaacg cgggtgatac
    10141 atagccgtaa gcgggcttta gccgtgtcgt cgtttcttcg acgagtgaga ccaacgtttc
    10201 gacactcccg gtctccgcgg cgctgagcgg aaatgtcatt tgcagcagcg tacgagaggc
    10261 ggcgccatct tcggccgcgt cgcgatccga gatcgtgaga ctgtattggg gggcgtgctc
    10321 tccggagtcg ttgatcgtga acgccgtgaa tcgccgcttg cgcgactttt tcccggttga
    10381 cgcggcgggc gtgagcgagc tccgcattcg atcgagcagc ttctcgtcga ccttcttcca
    10441 gtgctccgac gtcgcgctgg gcattgccca gcgaagctca cccgttggca ccgtcgcgac
    10501 gaagcgatcg aaaaccgtgg acagcgcctc gctcgcctcg tctatgaacg gctccaggaa
    10561 gaatgcggcg acaaccccgc tccgaactag tgcgtcaccg ttctcagaat cgcgaatctc
    10621 gatgggaacg accgggctcg gcatgacgag catccttccg cagctcgcac gccatgtcga
    10681 ggcccgacca cgacgtcgta ctaagaacct atcctaatca ctgggatcgg ttgctaagga
    10741 gttggcacct cggcacagtc ccacttaccg gatgcgcaca gacaatccaa gtccgcctcc
    10801 ttgtagtggc acggggcggc tgcctcattg tcctttgtca tgcacgcggc tccgtccttc
    10861 ggtggcatcg ccggacactg gttcattggc ttcggtggcg ccgtacaatg ccagcgtcgg
    10921 tcgggctcag ggccatcgca gttgcaggtg acttctgtaa actcgcactt gagcagcgtt
    10981 gtacaggttg ccttatcggc cggcggtgcc gagggacact cttcactcgt gccgccagcg
    11041 cccgctgagc cgccactatc cattccgccc attcccccgt ctgtgccggg gtggggtccg
    11101 tctggcgggc ccatggggcc atccggtttg cctggagctc ctccagcatc tgcaccacca
    11161 cttccgctct ctcctccgtc atcagggccc atgccgggct ctccgcccaa cgtcatgctg
    11221 gcgccaccaa gaccgccggg tcgagccccg ccgcttccca tcgtgcttcc agaacctgtc
    11281 gtcgaggttc cgccgatgcg tccacccatc gtaccgaacg agccagacgc gcctggaagt
    11341 ccaccggatg cgccgcccgt tccagagctc gtattgaggc ccccgtcact cgagccgcca
    11401 cacgccgcaa aagacgcgag aacgagcgat atcgttccgg gccagaagaa acggcgagcc
    11461 gtcgaagaca ctgcagttac gagttcaaca gagcgaggtg ttaccatcgc caccccaatg
    11521 ggaacttcca ggggactttc tgaacggtcg acgatttttg acgcagggtc gcggcccggc
    11581 cccgaggacc accccgccaa tcagcgtgag cctagaatga tacagtttgc gcgtgagcca
    11641 cgacttcagg atctatctca gcagtgatgt cttcccggat gcggcttggc tgactgtcat
    11701 gggtgtagtc agctcgaaaa gcatcccctg tccgcccgga tcttgctggc atcgccaatg
    11761 gatcaccgat tcacgagttt ggattacggt ccagtcattg gcagctaccc agatcttgag
    11821 tcccgcgatc cgattccccg tgagccataa ttgggcgggc ggcggccgcg gattgcctgg
    11881 gcatgaacgc agtgcagagc gcgcctagga gtgagagtgc gacgccgagc tgccccgaat
    11941 acgggaggtc gagagccatc ttgaatacca ggatactcag cactcccgcg ctcgatagcc
    12001 agagcggaag atgattgcct tgatgggcgg cgcggcgacg aacgctcgcg aggtgaacca
    12061 acatcagcac gacgaatacc ggcagcatcc acggcgagta cgggatcgaa ctaatccccg
    12121 cgacaccaaa tgcactcaga taggcggccc agcacaccgg gcacttggga aagaacgcgg
    12181 tcgcgagcgc gagccccggc ccggtcagca cgctgaccca acgttgtctg gtaggctgcg
    12241 gcaccaagtt ttgactcata ctgctggcga ccggcgactg atcgagtagt tgcgatcgaa
    12301 tcccggttgc ctcgtcgagc agcgcgcacg cctgaccgac tacggcgtcc tgatcccact
    12361 gtagacgttc gaaagcagca gcgatacgcc cgtgcgtgtc caagatgtac agctcgacgc
    12421 gatgtctgct gacgagagat tcgacgaagc tgacgccgag tccaaaaccg ctaacgagcg
    12481 tctcctgtcc ctcgacggcg cgcagcatcc gatgatcctc gctgagcgtt agtccgcggc
    12541 tgctcgcgta acccgctagg cgctcgggcg agtcccaggc ggggtcgtag ctgatcgcgg
    12601 cggtacgaat tcggctccca agcccacgct gctggagcgc gcgcacgagg tgaccgagtt
    12661 tggttaccgt aagtgagcac ttctccgggt tctcgcagcg cgtatagaag aacaccacga
    12721 tcgaagggtg acctacgaag acctcatcgt aggccacgcg ccgccccaag tgatcttcga
    12781 ataccactcc gctgagtcgg ccgcgctggc gtgtttcctt cgaccaggcc cgaaacgcgc
    12841 cgagcccctt caagaagtcg agtgaccccg aacaacactc gtgttggtcg ggtggcgagt
    12901 ccaccgcccg gagcgcggcg tccaccgctt ctgcgtccgc cgaagagagt tcaccgttcc
    12961 gctgggccgc aagactcttt aggcttggcg ccagtgactg cgcgcgagca cctaaccaag
    13021 tgatgctggc gagtacctcc cgaagagccg tggtgccaga cttgccaatc gcatatccgc
    13081 cgtagcgttc gaaggtgacg aggtcgtcgt gatatctaat gttcgagagg gccttgccca
    13141 ggtacggggc ccagtctgcc gatggggacg gctggctgcg cagggctcgc gccgcggccg
    13201 caactaggta cggatcgcga ccattgtcga gctcctcgag caagaagacg agatgtgacg
    13261 atgacagccg ccccatgcgc gtgagcacgc aaagtaccca gccgcgcata cgcaccacgg
    13321 cggctgcgcc gcgctccgaa tacacggtgt gactctcatc cagcagggcc ccaagctcct
    13381 cagtctgtcc gcgtcgagcg agcccctcga cctgctctgc gaactcgcgc tccgtaaggg
    13441 ggggagtgcg cggggccgat tcggcattgc cgcccagggt cgacatgttg gtttatactc
    13501 ggtgatacct agccaggaat gccttggcgc cgcgcttgag ctcgggtgag ccggaactct
    13561 tgaggtcggt cgtctttgcg cgcagctcgt catcccggcc catttcgtcg gccgccccga
    13621 aaagcgagag ggcggtcagg ctggcggcct tgacctcttc gtcttcgtca tcgtcgagca
    13681 cgatttcgcg cgcgtgcgtc tgtagcgcgg ccggcttcag cgcctgcagc gcggccgcag
    13741 agagccgacg aaattcagcg ggctcgttct tgtttcgcaa caccgattcg aacaaggcga
    13801 ccgagcctgc gtccgcgccc agcaggcgca gagcctcgcg tttggcctcg ggagtgggcg
    13861 gctttctcgc gatcttgcgc gccatgggat aagcctcggt gtgaacgtcg tagctcagca
    13921 actgcagtgc tttttccggt gaaaccaagg cggctttcgg atcctggagg ccttccaaga
    13981 gcagctgctg agcgtagccg tcgcgttcgc gaaccagaat gccaagcacg ctttggcgca
    14041 attcgggctc agcatcggag ctcatctcgc gcagcgtcgc cagatactcc gcccgccgtg
    14101 ggttgaacgc cacgacgctg aagctggccg cctgcagcgt ccgtaaggcc gtgctgcgta
    14161 gctcgggcgg ttcatcgggg tcttgcaaga tcgcgagcac ggcgttgaaa gttttgtctt
    14221 ccgcgcagac ggcaccagcc agcttctcga ggcctgcggc gcgcactctg acgctcttac
    14281 gccgatcttg cgcgtcactc agcgccgctt tggacgagac agctccgcgg gcgcgctcag
    14341 cgctggcttc ttctcggcgt gcgcgcgatt gtttggcctt acgcggctcg gccttctgcg
    14401 gttcggcctt gcgctgtgcg tccccggatt tgcgggcttt cgatttcttg gcgcgcatct
    14461 tcttttttct ccagagccgc gggctacaag tagttcacgt catcgtagtc gaaccccatg
    14521 cgagacgcac tcgcgagcct accctgataa tcgaggcagt ctcgcacgcg cggtgagccg
    14581 cctggggcca ctgccgtcgg ggacggcgcc agcgcaccac cgggtgcggt ggacggccgt
    14641 ggcgccgtcg taacaccatt ccaaggccac atcgtatcat ccacgttgtg acccacccgg
    14701 cttcccgtcg ccggtgcaaa cgcggccagt tgccccgggt tgaagcgatt gttggtgcgt
    14761 tgccacttgg cccacagccg gtcgacgttg gcatggagta gaaagaacag tggatctcga
    14821 gcggcggttg ggatgctcga gatgaagccc ccgaaactcg tgtgcgcaaa tccgtgggga
    14881 ttgccctcca tgcttcgaaa ggcagagtag ttcgtgccca gcgcaagcgt cgcggcctcg
    14941 ttgatcacga gggctgcttg actcctagta tcgaaccgcg gccgtcgatt gatgccgggt
    15001 acgccgtcag ttcgccagaa ctgtagggga ttcatggcgc taaatccgac cgtacccagg
    15061 cgatccgatg tgcccatgaa ctccggggta aagaggttcg gggccggttg atcgaagcgc
    15121 caataaggga gcgatacact cgaatcgatg gcctgcaact cgcgctccaa gtcgagcaga
    15181 taggctcgat gccagggtag aaaaccaggc gctccgtgcg cctcgggcga ccccgcctcc
    15241 gtatgcatat ttctgaagtc caaaaaccgc ccggccccgc ggttgttgag cgcggcgagg
    15301 gccgccaaga agcgatcacg ctcttgaggc gttagccgat tcgcgttctt tcggattcgc
    15361 accatcaagc gcacgtccaa cagcaccgct gcgccactca gtacttgaat tgcaacgtct
    15421 ccatccgcgg tcgaagcgcg aggaaagacc cccgacacga agaaggagac cgccgcggcc
    15481 ccgggtgaca gcgtaagcgt caaactggat tgaggcgcgc tggtcgcagt gcgccggaac
    15541 gcaaccctga tccccgtgcc aggccgattc cggacccgaa gggtggcggg cagggggggc
    15601 gtgaacggag gagttgccct cagaatgcaa ggggacggag cccacgtgac atagcgaccc
    15661 cgggcagacg cggtgttgtt gatggacaat tcgatggcca agattcaacc cctcggaatg
    15721 acggagtaga ttcccttact cgagtcccag taacaatcga caccatacgc gagtaagcga
    15781 catttgggaa ggagcagaca ctagaggagt gtgaaaatga atatttgacc atcgaggagg
    15841 acgtgcgaaa ccaccgtcgt ggccgtcctg ctggtctcag agcgtggcat tggggactgg
    15901 cgcggattct cgttcggcac cgtgaagtgc acgatgcaag gcgcagctca actcctcgag
    15961 cgtgccgggc ttcgccaaga attcgcgtac accgagcgtc ctcagatctt tcacgagcgc
    16021 cgggtcgagc agccccgacg tcatcacgac tggaatgttg ggagcgattc ggcgcagctc
    16081 tctgaccagc tcgagtcccc aaatatcggg catggagcaa tcgctaacga ccgcggcgaa
    16141 acgctcgggc gtattggcca acgctgccag tgcctggcgc ggatcagaga agctcaccgg
    16201 caaatagccg agtcgctcga gtagccgtga cgtggctctc aagacgggga gctcgtcgtc
    16261 gatgcagagt agctgctcgc cattcccggg catgcaattg gaggcgattt tgtcgtcgcc
    16321 aattacggca cgtgcgtcac gggcagggaa gtagatattg aattcagtgc cctgaccggg
    16381 cttgctgagc actaccatgc ccgcgtcgtg tgagcgcacc acgccgtgga ccacggataa
    16441 cccgagcccg gtgccggttc caggctcttt ggtggtgaag aagggatcga agatcttggc
    16501 cagtgtctcg ctatccattc ccgagccgtt gtctgcgaac gagagctgca cgtacaaccc
    16561 cggcttgagc tcgttgccgc cagccatgaa gcccggaccg atctcgaacc gcttgatgga
    16621 tacggtcaga attccggcat gtggcgctcg gttgaagtcc tgcatagcgt gcgccgcatt
    16681 ggtccccaag ttcatgatta cctgatgaat ctgagtggga tcggcgaaaa tctcggtcgc
    16741 atcagcggcg aagtgctcct cgattcggat cgatgcaggc aggcttgcgc gcagcaattt
    16801 caaggcctct cccactatat cgtgcaggca aatccagcgt ctttccggca cgccacgacg
    16861 gctaaacgtc agaatctgcc gcacgagcgc cgcggcgcgc aggctcgcct gcctgatctc
    16921 cgagagcgct tgctgaacgg ggtggttggg agccacgtcc tccgaggcca gcgcgacatt
    16981 ggccatcacc gcggcgagga tgttgttgaa gtcgtgcgcg atgccgccag tcagcg
//
LOCUS       DASTKU010001026         3425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1319552, whole genome shotgun sequence.
ACCESSION   DASTKU010001026 DASTKU010000000
VERSION     DASTKU010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319552"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="VFQ61_36395"
     CDS             complement(<1..991)
                     /locus_tag="VFQ61_36395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960047.1"
                     /translation="MKSSLVQRIEREDLVMFVNAGFACTGQREFYGDAHGQRVSIGFL
                     HEYILGNYRRLYARCLAAGLNHFNQALIISNLLATGDHTPRSLRAEEGELLFAALNAL
                     PTQRAYRVLAGLRERKINNRRTRAVVRRYLASRGDLTFDALKYRAKLRSAVAHCHLSL
                     PGECGKFLFECRHQRRFETPLFESYRSARYSKSALYDLPFTVAEGFAARHGVSRAGFL
                     REIEHQMTVGEKLRQQQAAARGQLSLQMDLGRVGLTRLASYALGLSVREREAARGRLE
                     AAFEAAARRALRRSPQRLGRVAAILDRSYSSAGSSEKRRRPLAVAWAASRILRL"
     gene            1489..>3425
                     /locus_tag="VFQ61_36400"
     CDS             1489..>3425
                     /locus_tag="VFQ61_36400"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9960048.1"
                     /translation="MAHLVVPRRILLWMDPQLATQVSEVLLGSSLEVQLAHVSGVGAI
                     EAEWRRPSDIIICDLAGARQIPEQVRRACPTLILIAEDFASAASATYRLQALYCPSSE
                     LGRLLEAVGAARPRGASSTHAFEDGQRELLERIAACEPLPGILDGIVRLIEDQGRDMV
                     CSILLLQDDGRLKHGAAPSLAPEFVRAIDGSPIGPEEGCCGAAAFRKMPIFVEDVLSH
                     EYWAKFRHLIQPLGFRGCWSSPIYSSDGAVTGTFAIYHREPRLPTEWELSLVARATHL
                     ASIAIERDRVEQALRRSEARYRQIVDTAYEGVWGLGRDGETWFANARAAELLGTTPEA
                     ILGRSVFEFVDEADRAQTEAYLRSRAQGRSEQSELRFRRVDGSDFWALVAASPICGDD
                     GTVVGWLGMLTDITQLKRTEATLRKSEEEFRSIFENAAIGMAVVGNAGRVLRSNGALQ
                     RLLGYRDEELSQMRFDMFTHPQDLAADLELYRRLSEGTIKSYQLEKRFIRSDGEIVWG
                     RFTASSVRVTEGEPLLGIGMIEDVTQSRLAQERIAWQAALLDQANDAIIVRDLDGQIQ
                     YWNDGAERLYGWSRREATGRTVQELIYRDASALVEPHRELRKRGVWTGELVHVTRSGK
                     ELAIRANWTLVRDEHGAAKSVL"
BASE COUNT          669 a         1041 c         1087 g          628 t
ORIGIN      
        1 ccaaacgcag gatacgactc gcggcccatg ccaccgccaa cggccgccgc cgcttctccg
       61 aagatcccgc agacgagtag ctacgatcta ggatcgccgc cactcgcccg agtcgctgcg
      121 gtgaacgtcg caaagcgcgc cgagccgcgg cctcgaatgc cgcctcgagc cggccgcgcg
      181 cagcctcccg ctcacgaacc gaaagaccga gtgcatacga agccaatcgc gttagaccca
      241 ctcgacctag atccatctga agggacagtt gtccgcgggc cgcggcttgc tgctgacgca
      301 gtttctcgcc cacggtcatt tgatgttcga tttccctcag aaaccctgcc cgcgagacgc
      361 cgtgtcgagc cgcgaatcct tccgccaccg tgaaaggcag gtcgtagaga gcagacttcg
      421 aatatcgtgc gctgcggtag ctctcgaaca gcggagtctc gaaccggcgt tggtgcctgc
      481 attcgaacag gaacttcccg cattcacccg gcagacttag atgacaatgg gctaccgccg
      541 aacgcagctt cgcacgatac ttgagcgcgt cgaacgtcag atctccgcgt gaagccagat
      601 agcggcggac gacggcgcgg gtgcgccggt tgttgatctt acgctcgcgc aatcccgcca
      661 gaactctata ggcgcgttgg gtcggaagcg cgttcagggc cgcgaacaac aactccccct
      721 cctccgctcg cagactgcga ggcgtatgat cgccggtcgc caaaagattg gagatgatca
      781 aagcctggtt gaagtgattc agccccgcgg cgaggcagcg agcgtacagg cgtctgtaat
      841 tgccgaggat gtactcgtgc aagaacccga tagaaacacg ctgaccgtga gcatcaccat
      901 agaactcgcg ttgccccgta cacgcgaacc cggcgttcac gaacatcacc aagtcttccc
      961 gttctatccg ttgtacgagc gagctcttca tggggcctcg atgaggatgc gttcgcatca
     1021 ctgacgacgc agagcgcggt agagaccgcc cggggagtgg aacacgcgac tagcttgctc
     1081 agcttcagag gccggtttcg gaagtcgcgt agaagtccca cgggcggcct ccacgatgtt
     1141 acgttatttc tgtcgtgttc gctcgaagcg tgagcacgag gtgtgccatg ggtaatatcc
     1201 gcacaaaagc gggcgctacc cagccgtcga gcccatcgac ctatccggaa gtataaggtt
     1261 tagcctaggc gcgactggtt ttgttttggc taaacctcag taccaggtgg gatggccagc
     1321 gtgttactcc cgcgggcctt gcttcctcta ttgggagccg tagcattgtc tcggctgtgc
     1381 gactcactcg cgggactcaa gtggaggaac gagaaaaatg acggctcggg acgtggctcg
     1441 agtctcgcgc atcccgcgtt gggggccgag cgcctaggag cctgtgtcgt ggcccactta
     1501 gtcgtacccc ggcggatcct gttatggatg gaccctcagc tcgcgacgca agtcagcgag
     1561 gtgctcctgg gctctagtct cgaggtgcaa ctcgcgcacg tcagcggagt aggcgcgatt
     1621 gaggccgagt ggcgtcggcc ttcggatatc atcatctgtg acctcgcggg cgctcggcag
     1681 attccagagc aagtccggag ggcctgcccg acactcatcc taatcgccga agatttcgcg
     1741 tccgcagcca gcgccactta tcgactgcag gcgctgtact gcccttcttc cgaactcggc
     1801 cgcctgctcg aggcagtcgg ggcagcccgc ccgcgcggag cctcgtcgac gcatgccttc
     1861 gaggatggcc agcgggagct cctcgaacga atcgctgcct gtgagcctct tcctgggatt
     1921 ctcgatggca tcgtgcgtct gatcgaggac cagggccggg acatggtgtg cagcatcctg
     1981 ctattgcagg acgacggtcg actgaaacac ggagctgccc ccagcctggc acccgagttc
     2041 gttcgggcga tcgacggctc accaatcggc cccgaagagg gctgctgtgg agcagccgct
     2101 ttccggaaaa tgccgatctt cgtggaagac gtgctcagtc atgaatattg ggccaaattc
     2161 cgccacttga tccaaccgct ggggttcagg ggatgttggt ccagcccgat atactcgagc
     2221 gacggcgctg tcacgggcac cttcgcgatc tatcaccgcg aaccgcgatt gccgacggaa
     2281 tgggagcttt ccctggttgc acgcgcgacg cacctggcat cgatcgctat cgagcgagat
     2341 cgcgtggaac aagcccttcg gcgaagtgag gcccgttacc gccaaatcgt ggacacggcg
     2401 tacgaggggg tttggggctt gggccgcgat ggggaaacct ggtttgccaa tgcgcgcgcg
     2461 gccgagctgc tcggaacgac accagaagcg atcttggggc gcagcgtgtt cgaattcgtg
     2521 gatgaggccg accgagctca gaccgaagcg tacttgcgga gccgagcgca gggacgcagc
     2581 gagcagagcg agttgcgctt tcggcgcgtc gacggctcgg atttctgggc cttggtcgcg
     2641 gccagtccga tctgcggaga cgacggcacc gtggtcggtt ggcttggcat gctgaccgac
     2701 atcacccagc tcaagcgcac cgaggccacg ctacgcaaaa gcgaagagga gtttcgcagc
     2761 atcttcgaga acgccgcgat cgggatggcg gtggtgggca acgccggccg cgtgctccgc
     2821 agcaacggtg cgttgcagcg gttgctcggt tacagggacg aggagctctc gcaaatgagg
     2881 ttcgatatgt tcactcaccc gcaagacctc gcggcagatc tcgagctcta tcgccgcctg
     2941 agtgaaggca cgattaagtc ctatcagcta gagaagcgct tcattcgaag tgacggtgaa
     3001 atcgtctggg gacggttcac cgcatcctcg gtgcgggtca ccgaggggga accgctactg
     3061 ggaatcggca tgatcgagga cgtgacccag agccggctcg cgcaagaacg aatcgcttgg
     3121 caggccgcgc tgctcgatca ggctaacgac gccatcatcg tgcgcgactt ggacgggcag
     3181 attcagtatt ggaatgacgg cgcggagcgg ctctacggct ggagccgtcg agaagccacg
     3241 ggccgcacgg ttcaggaact gatctatcgt gacgcatcgg cgctcgtcga acctcaccgc
     3301 gagcttcgga agcgcggagt ttggactgga gagctcgtgc acgtgacgcg ttcgggcaaa
     3361 gagctcgcca ttcgcgccaa ttggaccttg gttcgagacg aacacggggc ggccaaatcc
     3421 gtgct
//
LOCUS       DASTKU010001027         7881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_870949, whole genome shotgun sequence.
ACCESSION   DASTKU010001027 DASTKU010000000
VERSION     DASTKU010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7881
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870949"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFQ61_36405"
     CDS             <1..543
                     /locus_tag="VFQ61_36405"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET9960049.1"
                     /translation="DADMARVLAMAPSRAELLARADRGSELVLTAAASAIARLGKLPE
                     DAAVVVATYSASLEVNEIFDQRRRAGRPVEPRRFPATSPNLGAGLCTIAFELHGPSFA
                     ISCEQSLGPAALEIAAQVVAVDASAALLVFVEDVGAVVQDLLRSAGQPERPRVARALV
                     LERGSETVSTEPFWERWPAE"
     gene            700..1215
                     /locus_tag="VFQ61_36410"
     CDS             700..1215
                     /locus_tag="VFQ61_36410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960050.1"
                     /translation="MSLVPRVSLKSLLAVSIMAAPLSLAAVGLSGCSKQYIPNTEVEE
                     NDFNWRVVEFCEKYRRAVEKRNVALLLQMADSTYYEDGGTIDPSDDIDYAGLKDYLST
                     KFRETSSIRYEIRYRRVQPTDRSIVQVDYTYSASYKLPTEEGESWRRSVADNRLELRP
                     EGESFKIISGM"
     gene            complement(1224..1721)
                     /locus_tag="VFQ61_36415"
     CDS             complement(1224..1721)
                     /locus_tag="VFQ61_36415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960051.1"
                     /translation="MDPMLGFPRPASEGRTEDGLLVLATPPGMSAVRELLRLYFQALT
                     RESPEQLEPLLTEQAVLESSSGRHPARGALRAKFSQLDLAALDGVPLYRERDVQLFRR
                     GESDALLTDRAPELAPGQVFARVPLAVSHSGKTRLLPDRIGFLIQRGPSGYRIATITE
                     ELAAP"
     gene            complement(1989..2819)
                     /locus_tag="VFQ61_36420"
     CDS             complement(1989..2819)
                     /locus_tag="VFQ61_36420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HET9960052.1"
                     /translation="MARMFGFIGNRSDLGSWVLQSSADLLRVRRAPGEPLGWGFGFYQ
                     SGEILLRRRPMDDRSVIDLTEAAEDIRTDVLIGHVRRASVGALRTENTHPFRYRSWVF
                     AQTGTILGFDRLRERLLASQPEFLRRNVRGETDSECFFYLFLSFLHDAGHLSEQHVAP
                     GHVVTALRASIALVDRLSAEEGFGPNNSNILVTNGESMVAVHRDGLMGFRVLKGKFDV
                     EDLLGEEGVRRARIPSIESSHFTIIASELDELPQSWTEVKGRAFVTLSRTEDPVLEAL
                     "
     gene            3205..3975
                     /locus_tag="VFQ61_36425"
     CDS             3205..3975
                     /locus_tag="VFQ61_36425"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET9960053.1"
                     /translation="MASVSLAACSAVFPELSTPVRSAPPGFQFDPPPPRDLVYFAFSR
                     AVIPDRTRDGRAWDSVGGSLPDVFAKLVVDGTDLFVTPVQSNTLAPTWPNQKRGNYRV
                     RPGAQVIVELWDSNPLNNHPICSKEIKDLHDQVSVERSIEITCESGAFVELMAEPAHG
                     EIGTGLFYEIRTDGQVYVARVVGESPAARAGLRRGDQILKVMGEPVKSMDEGRVRSLI
                     NANASTGIGLMTRGTDGAEHELVVKDGPIYGEANERLR"
     gene            4005..4916
                     /locus_tag="VFQ61_36430"
     CDS             4005..4916
                     /locus_tag="VFQ61_36430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960054.1"
                     /translation="MPSILDSAKHGLVLLPASGRETLRALGRDAKTWLNGLVTSDLKP
                     VTAGQGGYGLLLSKQGKIQSDLEIVAQGESLLFTVAEGLGAGILESLEHFLIMEDVEL
                     SAAPELDWLRFVGRPELATRVLSGSSLEPHQLDWTGLGGTLCFVPKPSRGEILSKLAQ
                     SDDLVRATPEQAKFLSLACGFPEYGVDYGSDQNPHEAALDRRAVSWNKGCYLGQEVVC
                     MQDMRGKVKRRLVILELEAGSAPTRGAAISATEGGESVGELTSVASDAGRTLAFARMK
                     APHFESAQPLYVEGAGAARILAPATLG"
     gene            4935..5891
                     /gene="cysK"
                     /locus_tag="VFQ61_36435"
     CDS             4935..5891
                     /gene="cysK"
                     /locus_tag="VFQ61_36435"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET9960055.1"
                     /translation="MATDERGGESSSRITKSGLRVADDALALIAKTPLVRLRHVAPTG
                     VDLLGKAEFMNPAGSVKDRPALSMILAAERAGKLRPGSTIVEATSGNTGISLAMIAAV
                     RGYRCMLVMPEDMSLERRYILRAYGADIVLTPAADGMTGAVNRAQTLLTETPGAFMPS
                     QFKNPANPESHEQGTALELLEQTDGKIGLFVAGVGTGGTISGVGRVLKRELGSKVRVV
                     AVEPTNSAVLSGKTPGMHGIQGLGAGFIPEILDRSVIDDVVEVTDVAAERMVRRLARE
                     EGLLVGPSAGANVHAACDVAARAQVDGAVVTILCDSGERYLF"
     gene            complement(5944..6729)
                     /locus_tag="VFQ61_36440"
     CDS             complement(5944..6729)
                     /locus_tag="VFQ61_36440"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET9960056.1"
                     /translation="MNESPLLVSMAEGIAELSLNRPKTKNALDEALVASLGRALREAE
                     GNPAVRVIVISGQGGAFCSGVDLRTVLPELDRPERVAERLDAFHDVVRAICQSSKPVI
                     ASVDGAAVGFGADLALCCDLRVASEAAYFEEKFVALGLMPDGGGTYHFARMLGLGRGL
                     EYLLLGSRIDAAKASELGLVNRVVARDQLAAQTRNLADQLRRAAPLAVAAIKRAVRAG
                     QSGPLEDALARERTGQLELLASSDAREAIQAFLERREPVFHGR"
     gene            complement(6765..7259)
                     /locus_tag="VFQ61_36445"
     CDS             complement(6765..7259)
                     /locus_tag="VFQ61_36445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960057.1"
                     /translation="MKHYATAWVLGFSLWGCGGGTPPSPPPESGPFCGGIAAIRCPGL
                     GQCVDNPSDGCDPKRGGADCGGICECTALAKCAGGSRFDRSPSVCSCVPDSTQPEPNP
                     CAAVLCAANTQCVVEDGRAICKPIAAGVACGKNTCAAGQVCCNASCGICTPPDGVCIQ
                     IACE"
     gene            7454..>7881
                     /locus_tag="VFQ61_36450"
     CDS             7454..>7881
                     /locus_tag="VFQ61_36450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HET9960058.1"
                     /translation="MSRAFDIPAPIPLSEQRGQTYPALIEVMQRLLAPDGCPWDREQD
                     MASLRKYVLEEACEVIDAIDSGDRAHLSEELGDLALQVVFLGELARREGSFGPDDVVR
                     GIVEKLVRRHPHVFADAQVKDSEEVLSNWEKIKAAEKKQRP"
BASE COUNT         1433 a         2443 c         2560 g         1445 t
ORIGIN      
        1 gatgcagata tggctcgcgt gttggccatg gctccgtcac gtgccgagct ccttgcgcga
       61 gccgaccgcg ggtctgagct agtgttgacg gcggcggcga gcgcgatagc ccggttgggt
      121 aagttgcctg aggatgcagc cgtggtggtc gccacgtact cggccagcct cgaagtaaac
      181 gaaatcttcg atcaacgccg acgcgcggga agaccggtcg agccgcgacg cttccccgcc
      241 acttcgccaa acctgggcgc ggggctctgc acgattgctt tcgagctcca cggcccctct
      301 tttgccatct catgcgagca gtcgctgggc ccggctgcgc tcgagatcgc cgctcaagtc
      361 gtggccgtgg atgcgtctgc cgcgctgctc gtctttgtcg aggacgtggg cgcggtcgta
      421 caggatctgc tgcgcagcgc tgggcaaccg gagcgcccgc gggtcgctcg ggcgttggtc
      481 ttggagcgcg gctccgagac ggtttcgacg gagccgtttt gggagcgctg gcccgcagag
      541 taaactcccc gacgttttta tctgtcggcc cttgggtcga ttgccgatct tggcgctttc
      601 ccgcttcttg gtgcttgccg acagcgcccc ggctctttta cggtcccggg aattcgaaag
      661 cccggtgcgg cgtttcgagt ggtcgtcgaa agtcaaccca tgagcctcgt tccgcgtgtt
      721 tccctgaagt ccctactcgc tgtatcgatc atggctgcgc ccttgagcct ggctgcggta
      781 gggctcagcg gttgctccaa gcagtacatt ccgaacaccg aggtcgaaga gaacgacttc
      841 aactggcgcg tggtcgagtt ctgcgaaaag taccggagag cagtcgagaa gcgcaacgtc
      901 gctctgctgc tccagatggc agattcgacc tattacgagg acggcggcac gatcgatccg
      961 agcgacgaca tcgattacgc gggcttgaag gactatctct cgacgaagtt ccgcgagacg
     1021 agcagcattc gttacgagat ccgctaccga cgcgtacagc ccacggatcg ttcgatcgtc
     1081 caggtggact acacgtacag cgccagctac aagctaccca cggaagaggg agagtcctgg
     1141 cggcggtcgg tggcggacaa ccgcctagag cttcggcccg agggtgagag cttcaagatc
     1201 atctccggga tgtgagcgga gcttcagggc gctgcgagtt cttcggtgat ggtcgcgatg
     1261 cggtaaccgc tcgggccgcg ctgaatcaga aagccaatgc ggtccggcag cagtcgggtt
     1321 ttgccgctgt gggatacggc gagcggtacg cgcgcaaaca cctggcctgg ggctaactcg
     1381 ggcgcgcggt ccgtgagcaa ggcgtcgctc tcaccccgcc gaaaaagctg aacatctcgc
     1441 tcccggtaga gcggaacccc atcgagcgcc gcaagatcca gttgggaaaa cttggcgcgg
     1501 agcgcgccgc gcgcgggatg gcggcccgag gaagattcga gcaccgcttg ctccgtgagc
     1561 agaggctcga gctgttctgg actttcgcgc gtcaacgcct ggaagtagag gcgtagcaat
     1621 tctcgcacgg cgctcattcc cggtggcgtt gccaacacca acaagccgtc ctctgtgcgg
     1681 ccttcgctcg caggtcgcgg aaagcccagc atcgggtcca cggcgactcc gggcgcgcgc
     1741 ctcggcggcg gcttgtcggc gatgagtacc aacttcgcga tcggaggctc ggtcggagcg
     1801 gctgcgctcg ctgcgcagcc gctggcgaga gtgcccgaag ctagcaaagc ttcccgccag
     1861 cctagccgcg aaaggacgtc gggccacggt gtcaacccgc gccgccacgg tgttagcccg
     1921 cataaccgtg ttaaccgtgt cacaacatca tcctggctag atcgcgcggg cgtgaccgta
     1981 aggctctctt acagcgcttc gagcaccgga tcttctgtgc gcgagagcgt gacgaacgcg
     2041 cgccctttca cttcggtcca gctttgcggc agctcatcga gctccgaagc aatgatcgtg
     2101 aaatgcgagc tctcgatgct cgggatccgc gcgcgtcgca cgccctcttc gccgagcaaa
     2161 tcctcgacgt cgaacttacc cttcaagact cggaagccca tcaaaccatc tcggtgaacc
     2221 gccaccatgc tttcgccgtt ggtcaccagg atgttgctgt tgttggggcc gaagccctcc
     2281 tcggccgaca gccgatccac cagcgcgatg ctggcgcgca gcgcggttac cacgtgaccc
     2341 ggcgcgacgt gctgctcgga cagatgcccc gcgtcgtgca ggaaggagag gaacagatag
     2401 aagaagcact cgctatcggt ctcgcctcga acgttgcgcc gcaggaactc cggctgagac
     2461 gcgagcaacc gctctcgcag tcgatcgaag ccgagaattg ttcccgtctg cgcgaagacc
     2521 caagagcggt agcggaacgg atgtgtgttc tcggtccgca gtgcacccac actcgcccgc
     2581 cgaacatgtc cgatcagcac gtccgttcga atatcttcgg cagcttccgt gagatcgatg
     2641 accgagcgat catccatcgg cctgcgtcgc agcaggattt caccggactg atagaagcca
     2701 aacccccaac cgagcggctc gcccggggcg cgtcgcacgc gcagcaggtc ggcactcgac
     2761 tgcagcaccc aggaaccgag atcggaccgg ttgccaatga agccgaacat cctagccatc
     2821 ccgaggaatg ataccgctca gcgatggatt cggcatcctg tcttcacagg ctagttgagc
     2881 ccgggccagc ttcggcctgt tagccgacga tgtgcgacgc gggtcggcac gacatcgaga
     2941 gcgggtctga gcggctgagt ttcgtcggcg agagcttctc aggtggccgg ccagtccccg
     3001 gatgcaaccg gcgcgcgaaa gacggatgga ttttgcgttc aaccggcgcg aaagtgcggc
     3061 accgagcggg agcgttcgcg tccgctcggt tggtgctgcg tgggtgctct ccgcgcgatg
     3121 tgtcttcgaa ccgtgagtca gcccttgaac acacttaagc tcgctatccg gtcttttcgt
     3181 cggctgtccg ttggcctttt ggccttggcc agcgtttcct tggccgcgtg cagtgcggtg
     3241 tttcctgagc tgtctacgcc ggtccgatcc gcgccgccag gattccagtt cgatccacca
     3301 ccgccgcgag acttggttta cttcgccttc tctcgggcgg tgattccaga tcgaactcga
     3361 gacggtcgcg cctgggattc ggtgggcgga agcctacccg atgtgtttgc caagctcgtt
     3421 gtggacggta ccgatctgtt cgtgacgccg gtgcagtcca ataccctcgc tccgacctgg
     3481 cccaatcaaa agcgcggcaa ctaccgggtg cgtcccggcg cgcaagtcat cgtcgagctt
     3541 tgggacagca atccgctcaa caatcacccg atctgcagca aggaaatcaa agacctacac
     3601 gatcaggtct cagtggagcg cagcatcgag atcacgtgcg agagcggcgc gttcgtcgaa
     3661 ttgatggccg agccggcgca cggcgaaatt ggcaccgggc tgttttacga gatccgaacc
     3721 gacgggcagg tctacgtggc ccgggtcgtg ggcgagtctc ccgctgcgcg ggccggcttg
     3781 cggcggggtg accagatctt gaaggtcatg ggcgagcccg tgaagagcat ggacgagggc
     3841 cgcgtccgca gcttgatcaa cgcaaatgcg agcacgggca ttggtttgat gacgcgcggg
     3901 actgacggag ccgagcacga gttggtggtc aaggacgggc caatttacgg tgaggcgaac
     3961 gagcgcttgc gctaagaccc gccctgcgca acctgtctga ccgcatgcct tcgatcctcg
     4021 attccgcaaa acatgggttg gttctgctgc cggcgagcgg acgagaaacg ctgcgagcct
     4081 tagggcgcga cgcaaagacc tggctgaatg gactcgtgac gagcgacctc aagcccgtga
     4141 cggccggcca aggcggatat ggacttcttc tcagtaagca gggaaaaatc cagtctgatt
     4201 tggagatcgt ggctcaaggc gagagcctcc tcttcaccgt cgcggaaggg cttggggcgg
     4261 gcatcctgga gtcactcgag cacttcctaa tcatggagga cgtcgagctc tcggccgccc
     4321 ccgagctgga ttggttgaga ttcgtcgggc gtcctgagct cgccactcgg gtgctgtcgg
     4381 gcagctcgct cgagcctcat cagctggatt ggacggggct gggcggaact ctatgttttg
     4441 tcccgaagcc ctcccgaggc gagattttgt ccaagctcgc tcaatccgac gacctggtgc
     4501 gggccacacc cgagcaggcg aagttcttgt ccctcgcgtg cggcttcccc gaatacggcg
     4561 tcgactatgg ctccgaccag aacccacacg aggcggcctt ggatcgacgg gcggtgtcct
     4621 ggaacaaggg ctgctacctc ggccaagagg tcgtctgcat gcaggatatg cgcggcaagg
     4681 tgaagcgacg tctcgttatc ttggagctag aggccggatc tgcgcccacg cgcggcgccg
     4741 ccatttcggc taccgagggt ggcgagagcg tcggagaact cacgagtgtt gcgagcgacg
     4801 cggggcggac gctcgccttc gcacgcatga aggctccgca tttcgagagc gcgcagccgc
     4861 tttatgtcga aggcgcgggt gcggcacgga ttcttgctcc agctacgttg ggatgatgct
     4921 aactaatcag ggcaatggcg accgacgaac gtggcggtga gagctccagt cgaatcacca
     4981 aatcagggct tcgagtagcg gatgatgcgc tcgctctaat cgcgaagacg ccgctcgtgc
     5041 ggctcaggca tgtggcacct accggtgtgg atctgctggg caaggccgag ttcatgaatc
     5101 cagcgggaag tgtcaaggat cgccctgctc tgtcgatgat tctggccgcg gagcgcgcgg
     5161 gaaagttgcg ccccggttcc acgatcgtcg aggccacgag cggcaacacg ggcatcagct
     5221 tggccatgat cgcggcggtg cggggttacc gctgtatgct cgtgatgccc gaagacatga
     5281 gcttggagcg gcgctacatt ctccgcgctt acggtgcgga catcgtgctt acgcccgcgg
     5341 cggacggcat gaccggtgca gtcaatcgag cgcagacttt actcaccgag acgcccggcg
     5401 cgttcatgcc cagccaattc aagaaccccg cgaacccaga gagccacgaa caaggaactg
     5461 cgctcgagtt gctcgagcag acggacggca agattggcct gttcgtggcc ggtgtcggca
     5521 cgggcgggac aatcagcggc gtggggcgcg ttctcaagcg cgagctcggc tcgaaagttc
     5581 gagtcgtggc ggtggaaccg accaacagcg cggtgctgtc gggcaaaacc ccgggcatgc
     5641 acggcatcca gggtttgggg gccggcttca tcccagagat tctcgatcgc agcgtgatcg
     5701 acgatgtggt cgaggtcacg gatgtcgctg ccgagcgcat ggtgcgccgc ttggcgcgtg
     5761 aagaaggctt gttagttggg ccctccgcgg gtgccaacgt gcacgctgcg tgtgacgtgg
     5821 ccgcccgcgc gcaggtagac ggcgccgtcg tcaccattct gtgtgactcg ggcgagcgtt
     5881 acctgttctg agtcggcacc gctgactccg tcctaaatcg gcatccttcg cgactagccg
     5941 ccgctagcgg ccgtgaaata cgggttcgcg gcgctccagg aaagcttgga tcgcttcgcg
     6001 agcgtcggag gacgccagca gttcgagctg tcccgtgcgt tcgcgtgcga gcgcgtcctc
     6061 gagcgggccg ctctgtccgg cgcgtaccgc tcgcttgatg gcggcgacgg cgagcggcgc
     6121 agcgcggcgc agttgatcgg ctaagttgcg cgtttgcgcg gcgagctgat cgcgtgcaac
     6181 gacgcgattc accagtccga gctccgaggc cttggccgcg tcgatgcgac ttccgagcag
     6241 caggtattcc aagccgcgcc cgaggcccag catgcgcgcg aagtgatacg ttccgccgcc
     6301 gtctggcatc aacccgagcg cgacgaactt ctcttcgaag tatgcagctt cgctcgccac
     6361 gcgaagatcg cagcacaacg cgagatctgc gccgaagccc accgccgcac cgtcgacgct
     6421 cgcgatcacc ggcttactgg actggcaaat ggcccgcact acgtcgtgaa aagcgtccag
     6481 gcgctcagcg actctctccg ggcgatcgag ttcgggcagg accgtgcgca ggtcgactcc
     6541 agagcaaaac gctcccccct gcccggaaat tacgatcacg cgcaccgcgg ggttgccctc
     6601 agcttcacgc aaagcgcgcc cgagcgaagc caccaacgcc tcatcgagtg cgttcttcgt
     6661 cttcggtcgg ttcaggctca gttccgcgat accctcggcc atcgatacga gcagggggga
     6721 ttcgttcatg tgttgatcag aactcaggcg tccgctttca gcaatcactc gcacgcaatt
     6781 tggatgcata cgccgtccgg cggcgtacag atgccgcacg aagcattgca acacacttga
     6841 cccgcggcgc aggtgttctt tccgcaggcg acacctgccg cgattggctt gcagatggct
     6901 cggccgtcct ccacgacaca ctgcgtgttg gcggcgcaaa gcacggccgc gcaggggttg
     6961 ggttcgggtt gcgtcgagtc cgggacgcaa ctgcacacgc tcggcgaacg gtcgaagcgc
     7021 gagcccccgg cgcacttggc gagtgcggta cactcgcaga tcccaccgca atcggcgcca
     7081 cctcgcttgg gatcgcaacc atcgctggga ttatcgacac attgcccgag cccggggcaa
     7141 cggatggcgg caatgcctcc gcagaatggg ccactctcgg gcggagggga gggcggtgtt
     7201 cccccgccgc agccccacaa cgagaatccg agcacccatg cagtcgcgta atgcttcatg
     7261 gaaacctccg aaatacgcgc gaatcggcgc gctaccggga gcttacatcg gagtgtgagg
     7321 gcgagccccg aatcagtacg gaaaacactc gaggcaaaaa cgttttctgg tcgacacgcg
     7381 gccgggggcc aaacggggct cctcgccacg gcctagctgg taattctcgg cggccgaggt
     7441 agtaacgtgt tcgatgagtc gcgccttcga catccctgcg cccattccgc tctccgagca
     7501 acgtggacaa acctacccag cgttaatcga agtcatgcag cgcctgctcg ctcctgatgg
     7561 ctgcccttgg gaccgcgagc aggacatggc ttcgttgcgc aagtacgtgc tcgaggaggc
     7621 ctgcgaggtg atcgacgcga tcgactcggg cgatcgtgct cacttgagcg aggagctcgg
     7681 agatctggct ttgcaagtgg tgttcctggg tgagctcgcg cgacgtgagg gcagcttcgg
     7741 cccggatgac gtggtgcgtg gcatcgtcga gaagctggtt cgccgtcatc cccatgtttt
     7801 tgccgacgcc caagtcaaag actccgaaga ggtgctttcg aactgggaga agatcaaggc
     7861 ggcggaaaag aagcagcgtc c
//
LOCUS       DASTKU010001028        10741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1990522, whole genome shotgun sequence.
ACCESSION   DASTKU010001028 DASTKU010000000
VERSION     DASTKU010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10741
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990522"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..868
                     /locus_tag="VFQ61_36455"
     CDS             323..868
                     /locus_tag="VFQ61_36455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960059.1"
                     /translation="MKPSNAPCALPGPRAEVTVTVSAGGKPPRVSLRARMHLINPLDR
                     DVVLLFNDGLEGLESASSVIVSRTSSKPPVFLWTFSGAEIVDALPLAPKSDIVLNEVE
                     LDATRGAEAVSLTFVSDLRIAGRPARVWLRASGVSPRGGEFSLANLEQLLEKQADELN
                     PPPVTLRVLCTQTINVEAWTQ"
     gene            complement(946..1401)
                     /locus_tag="VFQ61_36460"
     CDS             complement(946..1401)
                     /locus_tag="VFQ61_36460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960060.1"
                     /translation="MTVHTEREPTEDEWARYMGGADAHKPLEEQRILVLSEGGSPNGT
                     QRKQLIELLADAKVPTAILTNNWLMRAAGAAVSWFNPSLKVFGPLALEAAMNYLQLTP
                     WERSESVRLIREFQRELGLPVLGRVSLAGLELHDGPISGARLPPEERAP"
     gene            1968..3188
                     /locus_tag="VFQ61_36465"
     CDS             1968..3188
                     /locus_tag="VFQ61_36465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9960061.1"
                     /translation="MQSNIESRRSLMGQRGQLLKTLGCALLLVAEGCSSNSAEPRASG
                     GSATSGGAANGSASGGSAGRAGGSAGLGSGGTSQAAAGTSAAGGKVSDGGMTGTGGSA
                     PGSGGAAGGAQGGGGAGGGVAGGGAPGGATAGGTNAGGTPNTGGKASGGAGAAAASGG
                     QGGAGSTSAGGTSAGGTTGSGGGGASYCPTTAGTPCNIMPIGDSITEGCCTAPMGGYR
                     IELYHQALTNKKNITFVGTLTNGPGMVDGQAFPQRHEGHGGWKISQIAGVVDKAISSS
                     HPHVVLLKIGTNDINGNDNIANAPMRLANLIDQICKDAPDALLVVSAIIPTTNDGTNN
                     NVRNYNTAIKQKAEAAAAAGKHVVFVDNYQAFAQNSNYKTALMADGLHPNTAGYAVLG
                     DSFYKVISSYLPAK"
     gene            complement(3281..5050)
                     /locus_tag="VFQ61_36470"
     CDS             complement(3281..5050)
                     /locus_tag="VFQ61_36470"
                     /inference="COORDINATES: protein motif:HMM:NF012836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960062.1"
                     /translation="MRNIRTKSRSLLGLAVAAVSGVVSLTNMAHASDPRLIPKPAPLN
                     SLGQEAGWSADLAGDFSLMGAYMLGERFADSGGAYLFDTGNFNARQTFRLTGPVTLNH
                     THYGIGGDGKVFASPPTQCTFTEAQVNGAYLGDRVTISSQWVALAAYDLGHANTPPHE
                     VANCAGQPGSVPAVFLAKRLPSGSPFPYSTLNYNFNVPFRERVQAMDISDTDLVLVGS
                     GNGAHIFSYNASQDKWNFNKTVLFAEDLRPEFGQIVSIDDDTIVIGNKDASKVHVYRK
                     QAGVWAFLTEYQSSRAGFGRAVDVKDDLLAVGSDEGVDFFAITGNSLTHINGILGTGV
                     KYVAINGEYAAGIEDNQSGYAWQYRNNPAGGGYYKSGTLGGGAVDLNGNVANWGAHNL
                     EIDGVRMLSGFKGYGNGTQILIGSVIFDSIKPLFAGTNTIDNAEGDRLWLNRGKYDWR
                     RHHGPTPTSGTGPDSGATGSQNYYYVETSQGEGAYYQGDDAILESDYLSTNSRVDFDF
                     HMYGSTMGTLYVEVLENRSNATWRPILTLDGSQSWIRSTSWQRRGVGLNGYVGPVRVR
                     FRYVARGGAGGDVGLDNIVVNRN"
     gene            5207..6034
                     /locus_tag="VFQ61_36475"
     CDS             5207..6034
                     /locus_tag="VFQ61_36475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9960063.1"
                     /translation="MGPSTSVLAAAPERCFREGSGRFSQIEWDESDFRVAWQQYSHLG
                     FNAERVEDDFVRLACLRGRPGAAEVLNEVYLLPLAEGIQRVCRSPELTDGVLQNLREK
                     LLLPPAPRLLAYQAPGNFRAWLKVVATSLALDVARELGIKRQREVELDEQLEALAAGP
                     EEQYVRAELQEAFREALRAAVKGLRERERQVLRMHLVAGWNITQIGRVFSVNKSTAAR
                     WLVAAKDQLHVLLCADLGRRLGANSGEDSLVLEEMPSRLDINLRSFFQTTGALAPLE"
     gene            6060..7568
                     /locus_tag="VFQ61_36480"
     CDS             6060..7568
                     /locus_tag="VFQ61_36480"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9960064.1"
                     /translation="MSSQHQCLSEETVLALVSGGFDEARVCALLAEAERCEDCSLLLH
                     EAGRAVDVDGTDGSDTLPWLNGAGVLRVGSIVASRFRVLRLLGRGGMGEVYEALDNEL
                     GELVALKTIRAIHATLRSSVERFRQEVRLARRAVHPNVCRVLEFGRHQVEGAVPVYFL
                     TMELLRGDLLSRHLRRRGRSSALESVAIARQICAGLCAIHEQGILHRDIKSSNIMLCS
                     GPAGPQSTSAQRAVLLDFGIARSLAFEATALSQGALIGTPDYMAPEQLQAQALSPATD
                     VYALGMVLFELLTGELPFAEDPTLTRALKRLQGSPPIPNTEATQLPPKLTRLVAHCLA
                     VSPSDRPQTAAALLDELAAIEQELSGSCVPSRSERAEAKLQNTVFTRARWLAALAIAG
                     GILLLIALGSRTTRPIAAVVSARPSHPPAGPASPPASAHANQSLPSPREATVVIPTPR
                     TGVAAPIARVATPARTAASARVARPPTPVAPSCSPPYYFDGDGFRVYRKECL"
     gene            7568..8461
                     /locus_tag="VFQ61_36485"
     CDS             7568..8461
                     /locus_tag="VFQ61_36485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960065.1"
                     /translation="MDRVIVSRARFAQVLALLGALTGLSNASAEPSAPVSTPEQPSDA
                     AADGCIAQHRQAQVERSHGRLLAAHEQLRRCLLPACSPILREACATLLADIERDTPSV
                     VLAAESPNGDLLNVTVEDAGKRIASRLDGVPIPLDPGEHQLEFRAAGMVPLNKVVVLR
                     AGDRNRRIAVRLEPVALEISPHATLPPALPPNAPTTTRSHVWNYTLIGAGSAVGVAAI
                     WMGASAMRDYRDAEDSCSPLCSEGRISAIHTKAIVADGLFVLSLATLSYGVFRILSTH
                     DAPRATSVLLGPGSISARGRF"
     gene            8458..10689
                     /locus_tag="VFQ61_36490"
     CDS             8458..10689
                     /locus_tag="VFQ61_36490"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960066.1"
                     /translation="MKALALLVCLLELGCSLRIETDKLNAGCAEGLRACNGRCLPVES
                     ACQCPEGEFEQSGACVPIAECTATQYEVSAPGVASDRQCRSLSDCPPGEFVARNSGRA
                     NDRVCRACPQGTFSDTENSARCIPFRDCAEDEYETAEPSAVRQRECTPLTRCVPGEFV
                     EREGSRTQDRVCAPCPAGSFTEGENGTACSPWTECRPGTVESETPTASHDRVCAACAH
                     TEYEALGQCLPLSDCAPGQYVSESATATSDRTFSPCSAQTFSTTENAPECNAVRACSA
                     GEIETKKPTLSRDRVCSACPAATYESAGACVPLTTCWPGFFVRQAPNNQRDRACQPCP
                     DGTFSETENAAACRGWQTCPSGAQQSVAPSRTNDRVCSACGDEHYEAGEGCVELTRCD
                     PGSFVSVPPTGLSDRQCSPCPAGTFSSTANPSFCTQLTDCRDNEKVATAPTSATDRVC
                     AACPSALKSRGPNATACEAGVLIELGTDRGCGLRESDGRVKCWGWESESFAPFAEETF
                     VAVSAGNHHSCAILASDRHPVCWGVDDSGGVKNAPKDASFAQIAAGNGHSCGILMDGR
                     IRCWGVDSRQAYDFGQVRDAPSGQFASISAGGSHNCALRAGTGEVVCWGYDAAKQVSG
                     APLGVSFESISAGEYANCAVRSLDGFVQCWGDDSQGLVTDAPKNIAFSKVSTAVAHAC
                     GLRRADRKVVCWGDDTDRKVSDAPQDIEFSDVAAGTWESCAVRASDGQVICWGYAWTP
                     ENF"
BASE COUNT         1963 a         3347 c         3436 g         1995 t
ORIGIN      
        1 tgatttatct gtgactcact tcgcggtctc gaaagtcgct cgtgcactgt tcctttgcgt
       61 cgtcgccgcg tgcgctttac tccagtgccg agcgcccgcg cgccctactc cggcggcgca
      121 tacgccgagc aagaacgtga gttcgagccc ccccgcgccc ggagccagga ctccccccag
      181 ccaggcaacc cccgccggcc tggagcctga tcccaaccca gcgcctgccc ctgacccagc
      241 gcctggtccc cggcaggcga ctgctcccaa ccctgcaact ggtcacggcc aagcgactgc
      301 tcccacccca gcagcgccac ccatgaagcc ctcgaacgct ccatgtgcgc tgccgggccc
      361 tcgcgccgaa gtcaccgtta ccgtgagcgc gggcggaaaa cctccgcggg tgtcgctccg
      421 cgccagaatg cacctgataa acccgttgga tcgagacgtc gtgttgttgt tcaacgacgg
      481 acttgaaggc ctggagtcgg ctagcagtgt catcgtgtcg cgcacttcgt cgaaaccccc
      541 agtgttcttg tggacatttt caggagcaga gatcgtcgac gcgcttccgc tcgcccccaa
      601 gagcgacatc gtactcaacg aagtcgaact cgatgccacc cgcggcgcgg aagccgtgtc
      661 cctcaccttc gtcagcgact tgcgcatcgc tgggcggccc gcgcgagtgt ggttgagggc
      721 gtcaggcgta tcgccaaggg gcggtgaatt ctccctggca aacctggagc agcttctgga
      781 aaagcaagca gacgagctca acccgccgcc agtcaccctg cgagtgctgt gcactcaaac
      841 catcaacgtc gaggcctgga cgcagtaaat catcgattca ttctggactt cgagtaccgg
      901 tgaagcagcg ggagccctga cgcgcgggga ccctctaaca atgctctaag gggcacgctc
      961 ttcggggggc agccgcgcgc ctgatatggg gccgtcatgg agctccagtc cggctaggct
     1021 cacccggcct agcactggga gccctaactc tcgctggaac tctcgaatca agcgcacgct
     1081 ttcgctccgc tcccaaggag tgagttggag gtagttcatc gcggcctcca atgccaacgg
     1141 gccgaaaacc ttgaggcttg ggttgaacca gctcacggca gctccggcgg cgcgcatcaa
     1201 ccaattattg gtcaagatgg ccgttgggac tttcgcgtcg gccaacaact cgatgagctg
     1261 cttgcgctga gttccattgg gggagccgcc ttccgagagc accaggatac gttgctcctc
     1321 gagcggcttg tgggcgtccg cgccgcccat gtaacgtgcc cactcgtcct cggtgggttc
     1381 cctctcggtg tgtacggtca agacaaaccg ccccagcctt ttccacatca tcgttcgctt
     1441 cgaccccgtg accattcctc gaggatagcc tgcgctcgaa aaattggaac aggtcatcct
     1501 aaaaacattg gaaaacccat cgaaccttga gctgacgctt cgagcatcgc aatggtttcg
     1561 cacgtccgag tgcagcaagt cctcggtacc taggacttta acctgcaata tttgcaatga
     1621 tgccgccgcg gatacagggg gcatctctgt aaagtgcgcg aaacgcggcc aagctcaccg
     1681 cggggcaacg acgagcgggt agggcgggtc tgcggccgcg ccggccccgc aaagttgttt
     1741 tcgacggacg aggggaaccg tgtatgttga gtcaaaccat cacccttaag cgccatccgc
     1801 ctcctaagcg cctcgtgatc gcggcttggc tcggtgcgct cggtcggcca cgtagttcga
     1861 acgcgccgcg gctttctgga acagtttggt gtaagccgtg aaagcggatg ttactgtgcg
     1921 cgcacacgct tgatcaacat ccgagtgcgg acacccaggc aggcctcatg caaagcaata
     1981 ttgaatcgcg tcgttcgttg atgggccaaa ggggtcaact cctaaagaca ttgggctgcg
     2041 ccttgctgct ggtcgcagag ggctgctcga gtaactcggc agagcctcgg gcctccggag
     2101 gcagcgcaac gagcggagga gcagccaacg ggagcgcatc gggaggcagt gccgggcggg
     2161 cggggggctc cgctgggcta gggagtggag gcacgtcgca agccgcagca ggcacgtcgg
     2221 ccgccggcgg aaaagtaagt gacggcggga tgaccgggac aggtgggagc gctccgggaa
     2281 gcggcggcgc agcgggagga gcgcaaggcg gaggcggcgc tggaggcgga gttgccgggg
     2341 gcggagctcc cggaggcgca acagccggcg gtacgaatgc tggcggaaca ccgaacaccg
     2401 gcggcaaggc ctccggaggc gcgggcgcgg cagcagcctc cggagggcaa ggcggtgctg
     2461 gcagcaccag cgccggtgga acgagcgccg gtggaacaac tggcagcggc ggcggagggg
     2521 ccagctattg ccccacgacg gccggtacgc cctgcaacat catgccgatc ggcgattcga
     2581 tcaccgaagg ctgctgcaca gctcccatgg gcggctatcg catcgagctc tatcatcagg
     2641 ccctgacgaa caagaaaaat atcaccttcg tcgggacgct gaccaacggt cccggcatgg
     2701 tcgacggcca ggctttcccc caacgtcacg agggacacgg cggttggaag atctcacaga
     2761 tcgccggtgt cgtcgacaaa gcgatcagca gctcgcaccc gcacgtcgtg ctgctcaaga
     2821 tcggcaccaa cgacatcaac ggcaacgaca atatcgccaa tgctcccatg cgcttggcga
     2881 acctgatcga ccaaatctgc aaggacgcgc ccgacgcgct gttggtggtc tccgcgatca
     2941 ttcccaccac caacgacggg accaacaata acgtgcgcaa ttacaacact gccatcaaac
     3001 aaaaggccga agccgcggcc gccgccggaa agcacgtcgt cttcgtcgac aactaccagg
     3061 cctttgcgca gaactcgaat tacaagaccg cgctcatggc ggacggccta caccccaaca
     3121 cggccggtta cgccgtgctc ggcgactcct tctacaaggt gatcagcagc taccttccag
     3181 cgaagtaaga gcgcggccct ttttcacatc ggcgtccccg cgaatactcc aattccgtcg
     3241 cactcgcggg gcaccgcttc aagcacacga ttccgtcagc tcaattccga ttgaccacga
     3301 tattgtccaa cccgacgtca ccgcccgcac ccccgcgggc gacgtagcgg aagcgtacgc
     3361 gcacggggcc gacgtagccg ttcaaaccga cgccgcggcg ctgccagctc gtgctgcgga
     3421 tccacgattg cgagccgtcg agggtcagga tggggcgcca ggtggcgttc gagcggttct
     3481 ccaagacctc gacgtacagc gttcccatgg tcgatccgta catgtggaag tcgaagtcga
     3541 ctcggctgtt ggtgctcaag tagtccgatt ccaagatggc gtcgtccccc tggtagtacg
     3601 cgccttcacc ttgggaggtc tctacgtaat agtagttctg cgatccggtg gctccgctgt
     3661 cggggccggt tccgctggtg ggcgtgggac cgtgatggcg acgccagtcg tacttaccgc
     3721 gattgagcca caaacgatcg ccttcggcat tgtcgatggt gttggtgccg gcgaatagcg
     3781 gcttgatgct gtcgaagatg acggagccaa tcaatatttg ggtgccgttg ccataaccct
     3841 tgaagccaga cagcatgcgc acgccgtcga tttccaggtt gtgtgcgccc cagttggcga
     3901 cgttaccgtt tagatcgacg gccccgcctc ccagggtgcc gctcttgtaa tagccacccc
     3961 cggctggatt gttgcgatat tgccaggcgt acccactctg gttgtcctcg atgccggccg
     4021 cgtactcacc gttgatcgcc acgtatttga cgccggttcc gaggattccg ttgatgtgag
     4081 tcaagctgtt gcccgtaatg gcgaaaaagt cgacgccttc gtcgctgccc acggccaaaa
     4141 ggtcgtcttt tacgtcgacg gcgcgtccaa agcccgcacg cgagctctgg tactccgtca
     4201 gaaatgccca tacgccggct tgtttgcgat agacgtgaac tttgctggcg tctttattcc
     4261 caatcacgat cgtgtcgtcg tcaatggaca cgatttgccc aaactcgggc cttagatcct
     4321 cggcgaagag gacggtcttg ttgaagttcc atttgtcctg gctagcgttg tacgagaaga
     4381 tgtgggcgcc gttgccactc ccaacaagga ctaagtcagt gtcggagata tccatggctt
     4441 gcacgcgctc acgaaagggc acgttgaagt tgtagttgag cgtgctgtac gggaacggcg
     4501 agccgctcgg caggcgcttg gccaggaaca cagccgggac gctgccaggc tgtcccgcgc
     4561 agtttgcgac ttcatgcggc ggagtgttgg cgtgtcccag gtcataggcg gcgagcgcaa
     4621 cccattggga tgaaatcgtg acgcggtcac ccaagtacgc tccgttcacc tgagcctctg
     4681 taaacgtgca ttgtgtcgga gggctggcaa atactttgcc gtcgcctccg atgccgtagt
     4741 gagtgtggtt cagtgtcacg ggtccggtca gtcggaatgt ctgccgcgcg ttgaagttgc
     4801 ctgtgtcgaa gagatacgcg ccgcctgagt ctgcaaagcg ctcgcccagc atataagcgc
     4861 ccatcaaact gaagtcgccc gctaggtccg cggaccatcc cgcttcctgg cccaggctgt
     4921 tgagtggcgc gggcttggga atgaggcggg ggtcgctggc gtgcgccatg ttcgtcaatg
     4981 agacgacgcc ggacacggcg gccacggcaa ggcccagcaa tgagcgtgac ttggttcgaa
     5041 tgtttctcat gtgctttctt ccaacgattg aacgaagcga ccacgcgcct ggtctcgctg
     5101 ctcggcgcac tttccagaaa taattttggg gctggttcgc cctgatccgc ttagttcgcg
     5161 gccgccaccg agttgcagtg atctaacttc gaggtattgc tggccggtgg gcccttcgac
     5221 ctccgtgctt gccgccgctc ccgaacgctg ctttcgcgag ggaagcgggc gcttttctca
     5281 aatcgagtgg gacgagagcg acttccgcgt cgcctggcag cagtattcgc acttggggtt
     5341 caatgccgag cgagtcgaag acgatttcgt gcgtttggcc tgcctgcgag gccggcccgg
     5401 ggcggcagaa gtgctgaacg aagtctacct tttgccattg gcagagggca ttcaacgcgt
     5461 ctgtcggagc ccggagctga ccgatggtgt gctgcagaac ctacgcgaga agctgcttct
     5521 gccacccgcg ccccgtttgc tcgcgtatca agcaccgggt aacttccgcg cgtggctgaa
     5581 ggtcgtcgcc acgagtcttg cgctcgacgt ggcgcgcgaa ctcgggatca agaggcagcg
     5641 cgaggtggag ctcgacgaac agctggaggc ccttgccgca gggccggaag agcagtacgt
     5701 gcgggcggag cttcaagaag cttttcggga ggcgctccga gctgcggtca agggtttgcg
     5761 cgagcgcgag cgccaggttt tgcgtatgca cctggtcgcc ggttggaaca tcacccagat
     5821 cggccgagtg ttctccgtga acaagtccac ggctgcgcgc tggctcgtgg ctgccaagga
     5881 ccagctgcac gtgctgcttt gtgcggacct ggggcgccgc ctgggcgcga acagcggtga
     5941 ggattcgctg gtgttggaag aaatgccgag tcgtctagac atcaatcttc gtagcttttt
     6001 ccagaccacg ggagcgttgg ctcctctcga atagcgccca atcctgcgcg gcgtaggcca
     6061 tgtcatccca acaccaatgt ttgtccgagg agaccgtgct cgcgctcgtg tcgggcggtt
     6121 tcgatgaggc tcgcgtctgt gcactgctcg ctgaagcaga gagatgcgag gattgctctt
     6181 tgctcctgca cgaagccggg cgggccgtcg acgtcgacgg cacggacggc agtgacacgt
     6241 tgccgtggct gaacggcgcc ggggtcttgc gggtcgggag catcgtcgcg tcccgtttcc
     6301 gcgtgctgcg tctgctgggg cgtggaggta tgggggaggt ttacgaggcg ctcgacaacg
     6361 agctcggtga gctcgtcgcg ctcaagacca ttcgagcgat ccacgcaacg ctgcgctcga
     6421 gcgtcgagcg cttcaggcaa gaagtgcggc tcgcacggcg tgccgtgcat ccgaatgttt
     6481 gccgagtgct cgagttcggc cggcaccaag tcgaaggtgc ggtgcccgtc tatttcttga
     6541 cgatggagct cttgcggggc gatctgctga gtcggcactt gcggcgacga ggtcggtcga
     6601 gcgcgctcga gagcgtggcg attgcgcggc agatctgcgc gggtttatgc gccatccacg
     6661 agcagggcat attgcaccgc gacatcaagt ccagcaacat catgctgtgt tctggtccgg
     6721 cgggcccgca gtcgacgtcg gcgcaacggg ccgtgctgct cgacttcggg attgcgcgat
     6781 cgcttgcatt cgaagctacc gcgctcagcc agggagcgtt gatcggaacc ccggattaca
     6841 tggcccccga gcagctacaa gcgcaggcac tcagccctgc gacggacgtc tatgccttgg
     6901 gcatggtgct tttcgagctg ctgactgggg agcttccgtt tgctgaggat cccacgctga
     6961 cccgagctct caagcgactt cagggatcgc cgcccatccc aaacacggag gccacgcagc
     7021 taccgccgaa gctcacgcgg ctggtcgcgc actgcttggc ggtatccccg tccgatcgtc
     7081 cgcaaacggc ggcggcgctt ctggacgagc tggccgcgat cgagcaagag ctctctgggt
     7141 cgtgcgtccc gagtcggtcc gaacgcgccg aagccaagct tcaaaatacc gtattcaccc
     7201 gggcgagatg gttagctgcg ctcgcgattg ccggtggcat tctcctcctc atcgcgctcg
     7261 gttcgcggac gacccggccg atcgccgcag tggtctccgc gcggccgagc catccgccag
     7321 cgggcccggc atcgccgccc gcgagcgcac acgccaatca gtccctgccg agcccaagag
     7381 aagccacggt cgtcattccg acccctagaa ccggagttgc cgcgccgatc gcgcgcgtcg
     7441 caacgccggc tcgtaccgct gcgtccgccc gcgtcgcgcg tccgcccacg ccagtcgcac
     7501 cttcctgctc acccccttac tacttcgatg gcgacggctt tcgagtttat cgaaaggaat
     7561 gcttgtgatg gatcgcgtga tcgtgagccg tgctcggttt gcgcaggttt tggccctcct
     7621 gggagcactc accggtttga gcaatgcttc tgccgagccg agcgcgcccg tctcgacgcc
     7681 ggagcagccg tcggacgcgg ctgccgacgg gtgcattgcg cagcatcggc aagctcaggt
     7741 cgagcgcagc catggccgtc tgctcgccgc acatgagcag ttgagacgtt gccttttacc
     7801 cgcatgcagc ccgatcctgc gggaagcgtg tgcaacgctg ctcgcggata tcgagcgcga
     7861 cacaccgtcc gtggtccttg ccgcagagtc cccaaatggc gatctgctga atgtgacggt
     7921 cgaagacgcc ggcaagcgta tcgcctcgag actcgacggc gtcccgatcc ctttggatcc
     7981 cggcgagcat cagctcgaat ttcgagccgc gggcatggtt cccttgaata aagtggtggt
     8041 tctgcgcgca ggagaccgga accgccgtat cgctgtgcga ttggagccgg tcgctctcga
     8101 aatatctcct catgcaaccc ttccgccagc cctgccgcca aacgcgccca ccacgacgcg
     8161 cagccacgtt tggaactaca ccctaatcgg cgccggctct gccgtgggcg tcgccgcgat
     8221 ttggatgggc gcctcggcca tgcgcgacta ccgcgatgca gaagactcct gctcaccctt
     8281 gtgtagcgaa gggcgaatca gtgccattca caccaaggcg atcgttgcgg acgggctatt
     8341 cgtgctttcc ttggccacac tctcgtacgg agtatttcgg atcctgtcga cacacgacgc
     8401 ccctcgcgca acgtccgtgt tgctgggtcc aggctcgatt tctgcgcgag gacggttttg
     8461 aaagcactcg cgctgctcgt gtgtttgctc gagctcggtt gtagtctgag gatcgaaacg
     8521 gacaagctga atgcaggctg cgcggaggga ctacgcgcct gcaatggacg ctgtttgccg
     8581 gtcgagagcg cttgccagtg tccagaaggg gagttcgagc aatcgggggc gtgtgtccca
     8641 attgccgagt gcacggcgac tcagtacgaa gtcagcgcgc caggcgtcgc gagcgatcgc
     8701 cagtgtcgca gcctctcgga ttgtccgccg ggtgaattcg tcgcgcgtaa ctccgggcgg
     8761 gcgaatgaca gagtttgccg cgcttgtccg cagggaacct tcagcgacac ggagaactct
     8821 gcccgttgca tcccgttccg tgattgtgcg gaggacgagt acgaaaccgc ggagccgagt
     8881 gcggttcgac agcgcgagtg cactccgctt actcgctgcg tgcctgggga gttcgtcgaa
     8941 cgcgagggga gccggacgca ggaccgagtc tgtgcacctt gcccagccgg gtcgtttacc
     9001 gagggcgaga atggaaccgc ttgctccccg tggaccgagt gccgaccggg tacggttgag
     9061 tccgaaaccc cgacggcaag ccatgaccgg gtgtgtgcgg cgtgcgcgca cacggagtac
     9121 gaggctctcg ggcagtgttt gccgctgagc gactgcgcac ctgggcaata cgtcagtgag
     9181 agcgccaccg ccacgagcga ccgcaccttc tctccgtgtt cagcgcagac cttcagcacg
     9241 accgagaatg cacccgagtg caatgcggta cgagcgtgct ccgcgggcga aatcgagacc
     9301 aagaagccca cactgagtcg cgatcgcgtg tgcagcgctt gcccggccgc gacatacgaa
     9361 agtgcaggcg catgcgtgcc gctcactaca tgctggcccg gcttcttcgt gcggcaggcc
     9421 ccgaacaatc agcgcgaccg cgcgtgtcag ccgtgcccgg acggcacctt cagtgaaact
     9481 gagaatgcgg ctgcatgtcg cggctggcaa acctgcccaa gcggagcgca acaaagcgtc
     9541 gccccgagca ggacgaacga tcgcgtgtgc tcggcttgtg gggatgaaca ttacgaagcg
     9601 ggcgaaggtt gcgtcgagct cactcggtgt gatccgggct ccttcgtcag cgtgccgccc
     9661 acgggcttga gcgaccggca atgctcgccg tgccccgcgg gcacctttag ctccacggcg
     9721 aacccgagct tctgcaccca actcaccgac tgtcgcgaca acgaaaaggt cgctaccgcg
     9781 ccgactagcg cgaccgaccg agtgtgcgca gcctgcccct ctgccctgaa gagccgggga
     9841 ccgaacgcta ccgcgtgtga ggctggcgtt ctgatcgagc tcgggaccga ccgagggtgt
     9901 ggactgcgtg agtccgacgg cagagtgaag tgttggggtt gggagagcga gagcttcgct
     9961 ccttttgcag aagagacgtt cgttgccgtg agcgccggca accaccacag ttgcgcgatc
    10021 ttggcatcgg atcgtcaccc tgtctgttgg ggcgtcgacg actcgggtgg tgtgaagaac
    10081 gcccccaaag acgcgagttt tgcccaaatc gcggccggaa acggccacag ctgcgggatt
    10141 ttgatggatg gtcgaattcg atgttgggga gtcgacagcc gacaagcgta cgactttggt
    10201 caggtccgcg acgccccgtc gggacaattt gcgagcatca gcgcgggagg ctcgcacaac
    10261 tgcgccttgc gggcgggaac aggcgaggtc gtatgttggg gatacgatgc cgccaagcaa
    10321 gtctcgggcg ctccgctcgg cgtctcgttc gaaagtatca gcgctggcga atatgccaac
    10381 tgcgcggtgc gcagcctcga tggcttcgtg cagtgctggg gcgatgacag ccaagggttg
    10441 gtgacggacg ctccgaagaa cattgcgttc agcaaggtga gcacagccgt tgcgcacgcc
    10501 tgcggcctgc gtcgagccga tcgcaaggtc gtctgttggg gagacgacac ggatagaaag
    10561 gttagcgacg ctccccagga catcgagttc agcgacgtcg cggccggaac ttgggaaagc
    10621 tgcgccgtcc gcgctagcga cggacaggtc atctgctggg gttacgcttg gacgccagag
    10681 aacttctgaa tcgagcgcct cggatcgcgt tgcgccgccg caaggggagg cggcgcaacg
    10741 c
//
LOCUS       DASTKU010001029         8147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1095326, whole genome shotgun sequence.
ACCESSION   DASTKU010001029 DASTKU010000000
VERSION     DASTKU010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095326"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2001
                     /locus_tag="VFQ61_36495"
     CDS             <1..2001
                     /locus_tag="VFQ61_36495"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET9960067.1"
                     /translation="APLTSSRGEPTAQVDQPTPAAPVADVVSESARGLRLGVRVFLFL
                     ILSMGSVLPLTLLGLTEAKRSAQREIDAADRQALAAARAAAAQLSVAMDAYVHAAEAF
                     SAHIAARHDFSHEALKQALAAHALNHREFLGAYVSDSNGTAILYIRGDGTFSNSRLNY
                     NDRGYFKEARSSGRVAVSEVHRGRFTRKLTVQIAAPMLDSQRQFLGITCSSVDLSHIT
                     SEAQRTISTMPQGRLMVVDGLGRVIADSEGSPNADPRDGSKVALFAPLRGVRAELRVG
                     KDPQGTDVRAVVVGLTGIVDSWRVVAMAPQAVAEAQANRVKIQVAILGVSLVAVMLAI
                     SFWLAAWLSKPLRALASSAEAVTRGKLDVLPTVVRNAPREMTRLTVAVRSMIVTLRTH
                     AQNLEARVQLRTQELSQALETLRKTEQSIRDDIDKARLFQEQLLPALPNHPEAQLAVC
                     YAPLEQVSGDIYDVVALSERHLRLFVADATGHGIQASMRTILLKSAYDRLKNRHVSPE
                     TLMAELNTYLVEEFPEGDLHCAACCVDVHLAEDHAQVIYSNGGNGPLFILAGDTPARE
                     HYSDGPLLGATIVEFPEPAYFRLERGETLLVCTDGLLEQWNAQRQRFDVERLMHFELE
                     SNDNASIAASRLMAAFHAFRGTRSMSDDVTALLLHLRPRPSA"
     gene            complement(2026..2427)
                     /locus_tag="VFQ61_36500"
     CDS             complement(2026..2427)
                     /locus_tag="VFQ61_36500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960068.1"
                     /translation="MSSPHDTDRAEPQFDDPNLAQRVEDLSVSQIDALPFGVVRLDAR
                     GKITLLSQTEARQSGLAGRNVRGLAFFTELAPCMGTPAFLNRFEQARRTGSLDIWFRQ
                     IGDFADAQRELEVRVLSAAAGGVWVFIRRST"
     gene            complement(2429..3754)
                     /locus_tag="VFQ61_36505"
     CDS             complement(2429..3754)
                     /locus_tag="VFQ61_36505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9960069.1"
                     /translation="MSATSNKREEPSADHRAEPESLREVNLELTRNNSELESLVLALS
                     KALQRSEAVAQVTLGSVHDFRNALTVIQGEAVFLAERLHEPEQLNSLNALDSACRQAT
                     AIARDMLHRLRSSHEQETLVDGSELVARCRELILRVAKTGVRCVFDLAPYVWPVAVES
                     HQLEAALVNLSANARDAMPNGGELSISVRNRTNPKPGMVGDYVAFTIKDTGVGMPPDV
                     LARATEPFFTTKGPQRGTGLGLAMVQAFVTAARGVLSIDSELGRGTRVEILLPRANCS
                     VPPSSALEREDYVSRIRQQVQTPWLRRILQAWRAACQELGLPRPARVEAALVEHKAHC
                     IVLKSDGSGAGAPRLRLLHLGVDLMELLAGGERGGASLTTDVVQNLAAGYRRALESGV
                     PSYEYARYSFGEGKPFEFERLILPAAASGARATHLFGVVRMSNPNEEGA"
     gene            complement(3884..4246)
                     /locus_tag="VFQ61_36510"
     CDS             complement(3884..4246)
                     /locus_tag="VFQ61_36510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960070.1"
                     /translation="MSSVHSRQVVLGSRLDVEREHRFEVRDPERFDRLQFAMRVLALL
                     RPDMAVTLYEGRRRLQVTRGRDWSVGRPANWAMVAIPKDASRHHITFALAEMAGVSET
                     AFVASLLARAAATPFSSS"
     gene            4269..4841
                     /locus_tag="VFQ61_36515"
     CDS             4269..4841
                     /locus_tag="VFQ61_36515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960071.1"
                     /translation="MVRRELPQFGVENDGGVVKNQQVSESEPNWGEAAHVEFDVRDRA
                     GFVTQARQRVVGLCTKYLQPSETTAQLIMTAQELLENVAKYSSGSGARFDFELRDECG
                     RPTVRMRTENPASPQHLHKAIEMLDEIIAERDPQGLYDRWVAQSGEREGSGLGLIRIR
                     AEAGLTLSYTNEADKLCLEVVGPAPAKRSS"
     gene            4841..5209
                     /locus_tag="VFQ61_36520"
     CDS             4841..5209
                     /locus_tag="VFQ61_36520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960072.1"
                     /translation="MNSVVQDVASEAFSTKSRLAGERLTVELVGCLDMETAPQLRRFL
                     GLLTPIAEGAMVKEFEFHTQDLYLMSSSAISLLAAWIKSLKELAAGCRVKFRTNPNLS
                     WQKRSLDPIRRLAVEVVSVE"
     gene            complement(5213..6343)
                     /locus_tag="VFQ61_36525"
     CDS             complement(5213..6343)
                     /locus_tag="VFQ61_36525"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HET9960073.1"
                     /translation="MSSERPLRVAVLCGGQSQEHEISLLSARFVLEGLDPSRFEAVLV
                     GIGKDGVWRLQAAAHLLAAPPDPRRISLDAGMPEVMLRKHGAGARLECVGSQAAGTGG
                     LELPAVIDVVFPVLHGPMGEDGTLQGLLELCGVPYVGGSVIGSAIGMDKDVMKRLLLQ
                     AGLPVLPYRTVKHADFVRAPLEIERELGALGLPVFVKPANLGSSVGVRRVASPEQLRS
                     ALEHAFEFDDKLVVEAGLVAPREIVCAVIGGAPARVSQPGEIVVDHPDGFYSYAAKYL
                     DDFGVQTRIPAALAPDQTERARTLALATFEALEGRGLARVDLFVSQRGELYVNEINTL
                     PGMTRISMFPKLWEHSGIGGRELVSLLIEDARLRSARRKRSA"
     gene            complement(6453..>8147)
                     /locus_tag="VFQ61_36530"
     CDS             complement(6453..>8147)
                     /locus_tag="VFQ61_36530"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9960074.1"
                     /translation="AVGATLFLLASGRPVHEGNADEQRRAAYREDARSVASLVPDLPP
                     VLVATVDRALLRDPSIRFQSAEQMRDSLLGTFHPADVAAAPFQSASRRPLSEAGIPRR
                     PQEPPQRTALSDSVQRHPTEQDRELGPVLSSRPGTALAVVSSPDTWAPRKRDLSPTSE
                     LLIGRDAGPLGWSFADTRVSRVHARVAWDERQGCFRATDLNSTNGMRVNAVRKTTMPL
                     SSGDVLRVGDTVLVVSQGPTMAELRATVEQAARSTATLLITGETGVGKEVISRQIHAW
                     SGRSGAFVPVNCGALPRDIAASELFGHAKGAFSGAASARRGVFHAAQGGTILLDEVGE
                     LPLELQPLLLRTLQEKAVRPVGTDHEQPIDVRVIAATNVKLDSAVAEGLFRADLYARL
                     AQIPIELPPLRQRREEVLALAATFATQASAQFAITADAAEALLLWSWPFNVRELENLI
                     RRWCAMTQPGTPLGLEFLHGVNPAMTLPFQERSASQAPSSNSSSSARSPSNSGTPARN
                     PLSDRTALEALLVSCEGNVSEVARRLNTTRAQVYRWMDRLGLDASKTRSSSPPRRA"
BASE COUNT         1541 a         2666 c         2552 g         1388 t
ORIGIN      
        1 gcgccgctca catcctcaag aggcgaaccg accgcgcaag ttgatcagcc cactccggca
       61 gcgcccgtgg ccgatgtcgt gagcgagagc gcgcgcggcc tgcggctcgg ggtgcgcgtg
      121 ttcttgttcc tgatcttgag catgggttcc gtgctcccgc tcacgctact cgggctcacc
      181 gaagcgaagc gatccgcaca gcgagagatc gacgcggccg atcggcaagc cctagccgcc
      241 gcgcgcgctg ccgccgcgca gctctcggtc gccatggatg cttatgttca tgccgctgaa
      301 gcattttcgg cgcatatcgc cgcgcgtcac gacttctctc atgaagcatt gaagcaagcg
      361 ctcgcggcac acgcgctcaa tcaccgggaa tttcttggcg cttacgtatc cgacagcaac
      421 ggcacggcga tcctgtacat tcgcggcgac ggcaccttct ccaatagtag gctcaactac
      481 aatgaccgcg gctacttcaa agaagcgcga tcgagcgggc gcgtggccgt gtccgaagtg
      541 catcgggggc gtttcactcg caagctgacc gtgcaaatcg cagccccaat gctggacagc
      601 caacggcagt ttctgggtat tacctgcagc tcggtggatc tcagtcacat cacgagtgag
      661 gcccaacgca ccatcagcac catgccccaa gggcgcctga tggtggtgga cgggctcgga
      721 cgagtgatcg cggactcgga gggttccccc aacgccgacc cacgcgatgg ctcgaaagtg
      781 gcgctgttcg cgcctctccg cggtgttcga gccgagctgc gcgtcggaaa ggatccgcaa
      841 ggcacggacg tgcgcgcagt cgtagtgggt ttgactggca tcgtggacag ctggcgtgtc
      901 gtcgcaatgg ccccgcaggc ggtggccgag gcgcaagcca atcgagtcaa gattcaggtc
      961 gccatcctcg gcgtatcgct ggtcgcggtc atgctcgcca tctcgttctg gctcgcggcc
     1021 tggctgtcga agccattgcg ggctttggca agttcggccg aagcggtgac tcgaggcaag
     1081 ttggacgttt taccaacggt cgtgcgcaac gctccccgcg agatgactcg gctcacggtg
     1141 gcggtacgct cgatgatcgt cacgctccga acccacgctc aaaacttgga ggcacgagtg
     1201 cagctccgca cccaagagct gagccaagcc ctcgaaaccc tgcgtaagac cgaacagagc
     1261 atccgagacg acatcgacaa agcgcggcta ttccaagagc agctgctgcc ggcgttaccg
     1321 aaccatcccg aagcacaatt ggccgtctgc tatgcgccgc tcgagcaggt cagcggcgac
     1381 atatacgacg tggtcgcgct cagtgagcga cacctacgcc tgttcgtcgc ggacgcgacc
     1441 ggccacggta ttcaggcctc gatgcgcacg atcttgctga agtccgccta cgatcggctg
     1501 aaaaatcgac acgtttcccc cgaaactctg atggccgagc tgaatactta tctcgtcgag
     1561 gagtttccgg aaggcgactt gcattgcgcg gcttgctgcg tcgacgttca cctggccgag
     1621 gaccacgcgc aagtgatcta ttcgaatggt ggcaacgggc cgttgttcat tttggcgggc
     1681 gacacgcccg cgcgcgaaca ctattcggat ggtccgctgc tcggcgccac gatcgtcgaa
     1741 tttcccgagc ctgcatactt ccgcttggag cgcggcgaga ccttgttggt ctgcacggat
     1801 gggctactcg aacagtggaa tgcgcaacgg cagcgcttcg acgtcgagcg cttgatgcat
     1861 ttcgagctcg aatcgaacga caacgccagc attgcggcgt cgcgcttgat ggcggccttc
     1921 cacgccttcc gcggcacccg ttccatgtcc gatgacgtca cggcactgtt actgcatctg
     1981 aggccgcgtc ccagtgcttg agcgactcga gcttcggctc gcgtttcacg tactgcgccg
     2041 aatgaagacc cagactcccc cggccgcggc cgagagcacc cgcacctcca gttctcgctg
     2101 agcgtcagcg aaatccccaa tctgccgaaa ccagatatcc aggctacccg tgcgccgcgc
     2161 ctgctcgaaa cgattcaaga aagcgggtgt gcccatgcac ggcgcaagct ccgtgaagaa
     2221 ggccaagcct cgcacgttgc gccccgccag cccggattga cgagcctcgg tctggctgag
     2281 cagcgtaatt ttgccgcgcg catcgagccg caccacaccg aaaggcaagg catcgatttg
     2341 cgagacgctc aggtcttcca cgcgttgcgc caagttgggg tcatcgaact gaggctctgc
     2401 tcggtctgtg tcgtgagggc tagacatatc aggctccctc ctcgtttgga ttcgacatcc
     2461 ggaccactcc gaacaagtga gtggcgcgcg cgccgctcgc tgccgccggt aaaatgagcc
     2521 gttcgaactc gaaaggcttt ccctcaccaa atgagtagcg agcatattcg tagctcggca
     2581 ctccagactc cagagcgcga cgatagcccg cagccaaatt ctgcacgacg tcggtcgtga
     2641 gcgaagctcc ccctcgctcg ccgcccgcga gtaactccat caagtcgacc ccgaggtgga
     2701 gcaaacggag ccgcggagcg cccgcgccgg aaccatcgct cttcagtacg atgcagtgcg
     2761 ctttgtgctc caccaacgcc gcttccaccc gcgccgggcg aggcaacccg agctcctggc
     2821 atgcggcgcg ccaggcctgc aagatgcgtc gcagccaagg ggtctgcacc tgttgacgga
     2881 tgcgagaaac atagtcttct cgctccagcg cagagctcgg cggcacacta cagttggctc
     2941 ggggcagcag gatttccact cgggtaccgc gcccgagctc actgtcgatc gacaacactc
     3001 cgcgcgcggc cgttacgaac gcctgcacca tcgccagccc taacccggtt ccacgctggg
     3061 gccccttggt cgtgaagaac ggctcggtcg ctcgagccaa tacgtcgggc ggcataccca
     3121 cgccagtgtc cttgatcgtg aatgcaacgt aatccccgac cattcccggc ttcgggttgg
     3181 tgcggttgcg gacggaaatg ctcaactccc cgccattcgg catggcgtcc cgagcattcg
     3241 cgctcaggtt cacgagagcc gcttcgagct gatgtgactc caccgccacg ggccaaacat
     3301 aaggcgccag gtcgaacacg cagcgcacgc ccgtcttcgc gaccctcaaa atgagctcgc
     3361 gacagcgggc tacgagctca ctgccatcga cgagcgtctc ctgctcatgt gagctacgca
     3421 atcgatgcag catgtcccgc gcgatcgcgg tggcttggcg acaagccgaa tccagcgcgt
     3481 tcaacgagtt gagttgctct ggctcgtgca agcgctcggc caagaacacg gcctcacctt
     3541 ggatcacggt caaggcattg cggaaatcgt gcacgctgcc cagggtgact tgcgccacgg
     3601 cctcgctgcg ttgcaaagcc ttgctgagcg ccaggaccaa gctctcgagt tcactgttgt
     3661 tgcgggtgag ctcgaggttg acctcacgca ggctttccgg ctccgcgcga tgatcggcgc
     3721 tgggctcttc cctcttgttc gatgtggcgc tcaatcgacc ggaccgtcgc ggctcggctc
     3781 aggccgaagc tgattcgcgg cggcctcccc tgccgcgaat cgtggtgcca cgtgcccgcg
     3841 aatgcaagcc cacccgcccc gccaaggcgc gcggaaatga cgactagctg gagctgaatg
     3901 gcgtcgcggc cgcgcgcgcg agaagcgagg cgacgaacgc cgtctccgac acaccggcca
     3961 tttccgccaa agcgaacgta atgtggtggc ggctggcgtc tttcggaatc gccaccatcg
     4021 cccagttagc aggacgcccc acggaccaat cgcggccgcg cgtgacttga agccgacgtc
     4081 gcccctcgta caaagtcacg gccatgtcgg ggcggagcag cgctaaaacc cgcatcgcga
     4141 actgcagtcg atcgaagcgc tcgggatcgc gaacctcgaa ccgatgctct ctctcgacgt
     4201 ccaaacgact gccaagcacg acctggcgac tatgtacgga agacacgctg cacagtttga
     4261 cagattgaat ggtgcgccgc gagctacccc aatttggggt tgaaaacgac ggcggcgtgg
     4321 tcaagaacca gcaggtgagc gagagcgagc cgaactgggg cgaggcagcc cacgtcgaat
     4381 tcgatgttcg cgatcgggca ggtttcgtga cccaagcgcg ccagcgggtg gttggccttt
     4441 gcacgaaata cctacagccg agcgagacta ccgcgcagct gatcatgacc gctcaagagc
     4501 tgctcgagaa cgtggcgaaa tattcgagcg gctcgggggc gcgcttcgac ttcgaattgc
     4561 gggacgagtg cggccggccc acagtgcgca tgcgcacgga gaatccggcg agcccacaac
     4621 acctgcacaa ggcgatcgag atgttggacg agatcattgc ggagcgtgac cctcaaggtt
     4681 tatacgaccg ttgggtggct caaagcgggg agcgtgaagg ctcggggctc gggctcatcc
     4741 ggatccgagc ggaagccggg ctgacgctct cctacactaa tgaggctgac aagctgtgtc
     4801 tcgaggtggt cggaccagcc ccagccaagc ggagctcgtg atgaattcag tcgttcagga
     4861 tgtggcctcg gaggcgttca gcacgaaatc ccgactggcg ggcgagaggt tgaccgtcga
     4921 gctagtgggg tgtctggaca tggagaccgc gccccagctc cgacgattcc tgggcttgct
     4981 cacccccatc gcggaggggg cgatggtgaa ggaattcgaa ttccacactc aggatttgta
     5041 cctgatgagc tcctccgcga tcagtctgct cgcagcgtgg atcaagtcgc tgaaggagct
     5101 ggcggcgggc tgccgggtga agttccgaac caacccgaat ttgagctggc aaaagcgatc
     5161 gctggatccg attcgccgcc tcgccgtcga ggtggtctcg gtggaatgaa gattacgccg
     5221 agcgtttccg gcgcgccgag cgcagccgtg cgtcctcgat cagcaggctg actagttcgc
     5281 gcccgccaat gccggagtgc tcccaaagct tcggaaacat ggagatccga gtcatacccg
     5341 gcagagtgtt gatttcgttg acgtagagct caccgcgctg actcacgaac aaatcgacgc
     5401 gtgcaagccc acgaccttcg agcgcctcga acgtggccaa ggccagggtg cgcgcgcgct
     5461 ccgtttgatc cggggcgagc gcagccggaa tgcgtgtctg cactccgaaa tcatccaggt
     5521 atttggcggc ataggagtaa aagccgtcgg ggtggtcgac gacaatctcc cccggctggg
     5581 acacccgcgc cggggcccca ccgatcaccg cacatacgat ctcacgcggc gcgaccagac
     5641 ctgcttcgac taccagtttg tcgtcgaact cgaaagcatg ctcgagagcg gagcgcagct
     5701 gctccgggct cgcgacgcgg cgcaccccaa ccgaggaccc gagattggcg ggcttgacga
     5761 acaccggcaa cccgagggcc ccgagttcgc gttcgatctc gagcggcgcg cgcacgaaat
     5821 cggcgtgctt cacggtcctg tacggcagca cgggcaagcc ggcctgcagg agcaagcgct
     5881 tcatcacatc tttatccatg ccgatggcgg aaccaatcac cgagccgccg acatagggta
     5941 cgccacacaa ttcgagcagc ccttgcaggg taccgtcttc gcccattggt ccatgcaaca
     6001 ccggaaaaac tacgtcgatg actgcaggca actccaggcc gccggtgccg gccgcctgcg
     6061 accccacgca ctcgagccgc gcgcccgctc cgtgcttacg caacatgacc tcgggcatgc
     6121 ccgcatccag gctgatgcgc cgtgggtcgg gcggtgcggc cagcaaatga gccgccgctt
     6181 gcagacgcca caccccgtct ttgccgatac ccacgagcac cgcctcgaac cggctcggat
     6241 ccaggccttc cagcacgaaa cgcgcggata gcagtgaaat ctcgtgttct tgggattggc
     6301 cgccgcacaa cacggcgacg cggagggggc gctcactcga catgccggcg cagcctaccc
     6361 gatgcgtggc ctattggcca aaactgcgct ccgtcgcggg ggagacgaga gcttgcgagg
     6421 cttgccggga ggccacccgg cgagaagcga actcaggcgc gccgcggggg ggacgaactg
     6481 cgcgtcttcg aggcgtccag gcccaagcga tccatccagc gatagacctg ggcgcgcgtc
     6541 gtgttcaggc gccgagcgac ctcggaaacg ttgccctcgc agctcaccag caaggcttcc
     6601 aaggctgtcc tatcactcaa agggttacgc gcgggcgtac cgctattgga tgggctgcgg
     6661 gccgagctgc tgctgttcga ggacggagct tgggacgcgg agcgctcttg aaaaggtagc
     6721 gtcatcgccg gattgactcc gtgcaagaac tcgaggccga gcggggtgcc cggctgcgtc
     6781 atggcgcacc agcgccggat caagttctcg agctcacgca cgttgaaggg ccagctccaa
     6841 agcagcaatg cttcggccgc gtcggctgtg atcgcgaact gcgcagaagc ctgagtggcg
     6901 aacgtcgccg ccagcgcgag aacctcctcg cgacgttgac gcagcggcgg aagctcgatt
     6961 gggatttgcg cgaggcgcgc gtacaaatcg gctcggaata gcccctcggc gacagccgag
     7021 tccaatttga cgttggtcgc ggcgatcact cgcacgtcga tcggctgctc atggtcggtg
     7081 cccaccggtc gcaccgcttt ctcttgcagc gtccgcagca gcagaggctg cagctcgagc
     7141 ggcaactccc cgacctcgtc gagcaagatg gtgccgcctt gagccgcgtg aaacacccct
     7201 cgccgcgcgg aagccgcgcc cgaaaaggcg cctttggcat gcccaaacag ctcggacgcc
     7261 gcaatatcgc gcggaagagc gccgcagttt acgggcacga acgcgccgct gcgcccgctc
     7321 cacgcatgaa tttggcggga aatcacctct ttcccgacgc cagtttcacc ggtgatcagc
     7381 aacgtggccg tggagcgcgc cgcttgctct acggtggcgc gcagctcggc catggtcggg
     7441 ccctggctca cgaccagcac cgtatctccg acgcggagca cgtcgcccga tgacagcggc
     7501 atggtggtct tgcgcacggc gttgacgcgc atgccgttcg tgctgttcaa gtccgtcgcg
     7561 cggaagcagc cttggcgttc atcccaggcc acgcgcgcgt gcacccgcga gacacgcgta
     7621 tcggcgaagg accagcctag cggaccagca tcgcgcccga tcagaagctc actcgtggga
     7681 gagagatcgc gcttgcgcgg ggcccaagta tccgggctgc tcacgaccgc gagggcggtc
     7741 ccggggcggc tcgatagaac gggccccaac tcccgatctt gctcggtcgg gtggcgctgc
     7801 acgctatcgg acagagccgt gcgttgaggc ggctcttggg gccgccgcgg tatgcccgct
     7861 tccgacaatg gccggcgact ggctgattga aacggagcgg ccgcgacgtc ggccgggtga
     7921 aaggttccca gcagactatc gcgcatctgc tctgcgctct ggaaacgaat gctcgggtct
     7981 cgcagcaacg cgcggtctac cgtcgcgact aacaccggcg gcaaatcggg gaccaggctc
     8041 gcgaccgatc gagcatcctc ccggtaggcc gcgcgccgct gctcgtctgc gttgccttcg
     8101 tgtacgggtc gcccgctcgc caagagaaag agcgtggcgc ccacggc
//
LOCUS       DASTKU010001030        10452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_647789, whole genome shotgun sequence.
ACCESSION   DASTKU010001030 DASTKU010000000
VERSION     DASTKU010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10452
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647789"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(658..1809)
                     /locus_tag="VFQ61_36535"
     CDS             complement(658..1809)
                     /locus_tag="VFQ61_36535"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9960075.1"
                     /translation="MRERIDVEGAGMDPARVARIIERFRAQQATGVFPGGQLVVRRRG
                     RLVIDEAVGVARGYRDADHEPRQEFTPEQRSCVFSAGKPLVAIAIALLEQAAAIDVTR
                     PVAHYWPEFANAGKRELSVLDVLLHRSGLYLREIERDWRHFGDWHSVMSRIAATEPAF
                     PRGTLAYQPLGFGWILGELVRRISAKPIERFLEQDVLAPAGLEDLRLGLPRAEVGTIA
                     RSYWVDEKPPVLGGEQMVGFEEAQNSVEQLTAILPGAGTIGTARSLARFYAWLLDGTP
                     TLSGHRLIGESVLTKYITRQTRGIDRTVRLPLVLGRGFGMGWFWPHPYGWWRTSSCFG
                     HAGNFSVLAWADPVTRCAIAVVTNGNRAPTSLVSRFAGIGSAVRDACLD"
     gene            complement(1839..2342)
                     /locus_tag="VFQ61_36540"
     CDS             complement(1839..2342)
                     /locus_tag="VFQ61_36540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET9960076.1"
                     /translation="MQSSRKSRPLSEDAIERALEQFRSIVRGRSLRASQVREQVARAA
                     LAQPGHFSVDDLCYELDDKGAKDAHMATIYRAIPLLIEAGLIQPALMSQGEGQRYEVI
                     FEKDHHDHLVCTGCGRIVEFRSEALEALQRDIADRYEFLLDDHVLELLGRCKNCRATA
                     PKPRGIH"
     gene            complement(2508..3788)
                     /locus_tag="VFQ61_36545"
     CDS             complement(2508..3788)
                     /locus_tag="VFQ61_36545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960077.1"
                     /translation="MSNPEGPPESNCVVAAARALTERYSGVGRRAIISALVATCVSCG
                     SSSEPSATADARGNAEEQSLYALGSLVFNAEGTTSYVSLLSSLNKGNVDLSAAREFAG
                     LADLWVFDESIFVASNENLSITRFEVVGGALEERGSVNFGAYGLTNLGFFLNTFISPS
                     KAYLLNGSSEFIVWNPQTMRVTHTAPLPKFEKRGSLRPFPGYSDRAAQVRAGHLYQPI
                     YWTDDSFFEFDASSQIVVFDTTSDSLIEVLDAPCPGLDYSTRDGEGNLYFSNWVYAAG
                     GAAVLGQTDTCVFRVPAGSEPPHVAFTFSELTGGHQGAAFRVLDDGRTFFSVLYADRP
                     ELAGETDASKITSGANWRFWSKGQGNTSASMLANIDWNAGAQYSFSVDGKTYILLSTA
                     DYSATDVYAVASAKADLELAFRAEGWSTRLFRVR"
     gene            3960..5276
                     /locus_tag="VFQ61_36550"
     CDS             3960..5276
                     /locus_tag="VFQ61_36550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960078.1"
                     /translation="MKGRSRETSLLGGLVIAALGPQLACGSEASPQHASAGATDVASG
                     GTKAEGGHSSRGGETSAGGASATSSSRPLYVLANEVSSGDDTISYVSVLESLDIENVD
                     LSRAVEFPGGRATISTYSGWVFAAPPDSGEVHRYRVTERGALEEDGVISFLNYGFDTA
                     ILDAWGNTFVSATKAYLHHPSEGTSVIWNPSLMRIEGEVDLGGFELIRPELGDINGGT
                     GVVRGNRLFRTVFWTNWDEEQTSAEQYLAVYDTDSDRLIALHEERRCPGLSNRVSGDE
                     AGNFYFSNWIYNVGETVLRDAPESCVLRLAPGSERLDDFRFQYSRVTQGLQGANFAYL
                     ADAQAVMSVFYPERSPVSADSTPISLVSEPAWKVWSVDLEGKSKPVEGLDWMLGAFTP
                     VHLDGRSLLLIPGEDWSIVYVNELVDGKAVPRFEIPGWSYQLLKVQ"
     gene            5442..6653
                     /locus_tag="VFQ61_36555"
     CDS             5442..6653
                     /locus_tag="VFQ61_36555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960079.1"
                     /translation="MATPPRTSRGEPAPKLFLSCGKDCFEPYLRQELSYFDFTRDPYL
                     ADITVVIVRQPSGSAGERFTVTLTGRGVRPSGAKSFSVAAGTPVHAARQALLQLILRR
                     LLEELAGSEHETAFRLELPRRNGAVLSTLADTWDYWVISPELRGEGEGGSGFYFFQGV
                     GALTLRRITSLSKLRWRTSYTRRWSGFLLEDGSRIRGDTHGWDSRLIYARSLGERWAL
                     GGVVTGRTSEFENLEGHVHGGPLLELNLFPYAENVTRQLRFAYQTGAWGNWYIEPNRA
                     RAIRELRPYHAVSIIADLNQRWGSVQWVGQLNSFVDRPKAFRLSSGTVFSLRLFEGFS
                     VQTEGKIAWVRDLINLRGRPINDQELLLWTAEQPTGYTFEVKFGVSYTFGSIHNTIVN
                     PRFARVDLDDE"
     gene            6759..7046
                     /locus_tag="VFQ61_36560"
     CDS             6759..7046
                     /locus_tag="VFQ61_36560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960080.1"
                     /translation="MVSSLLNSRLGSATIALVASFVCALIVVAGASKWFPPGAAGLDQ
                     IVVPLSVFPIVWAAFVLVLHVAQHRARAWCFVAAITALHAVLIAKGLLSSS"
     gene            7176..8861
                     /locus_tag="VFQ61_36565"
     CDS             7176..8861
                     /locus_tag="VFQ61_36565"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HET9960081.1"
                     /translation="MKQPAASTSTSNTPANFEIGARGDTPRKRLYLLHRWLGVVTAFV
                     GALVFFSGAIATFHSELDAWSSRANRYPDVASLPGFNLDSAYLAARRGIESELLESTD
                     IAQAAGHPVSFFFHRHEPDGMGTREVGVLVELDPESLEVLSRRAGDGDDVTRATRWYA
                     LGDFFIRLHLYLLMPETLGLILTGTVGFALILLISSGIWVHRPNLKRLVRRPRSERPR
                     FLAADTHTLLGSWSLPYTLMVALTGAFFSFAGTVLVPAVAIVAFNGDQNALVETLVGH
                     VAVSERSGTASLEAIRRDSLQRCPVCRFERVSLESWGKREASATVDLSSSSLTRHVER
                     HLVYDGHDGAFLQEKPLLGTRPSLGNSVLALMADLHFGTLFGLLTKLLWAVSAVAACG
                     LAASGLIVFTTRSKAPHSGGTLLVRTLTIVILGGLPLSSGSSVYAWFLGIGLQLDAPL
                     GLMALAFGVAVLATAGLGLARWPLRKALGVALGSAGLLFSLSPWVALSITDLDLPQIW
                     FDPALRPTLWVDGVLSGLGLALLLIAGRINRMPSNAVQCSPPAIGPAQVAAGD"
     gene            8977..10326
                     /locus_tag="VFQ61_36570"
     CDS             8977..10326
                     /locus_tag="VFQ61_36570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960082.1"
                     /translation="MKSSSCSYSTLSSKRARVYSSVLAFGVSAAIGCSESEAGNPSLA
                     AGAASGGNPSSGKPGTGGSGAGGEGGAKQGQEPPRKVLGSIVIDADGNRVSYAQIVGE
                     LSGHFTNRDGIEVSGNANFLTHGSDFFYGLAESPEWVRYSTENGFRETGRLSFLNFGM
                     SYIDFANAIVDDETAVSVLTEAYVAVVWNPKSMKIKGTIDLAYLKKDGFSLEAFTTVA
                     HEGLVYVPAKWVNWETPEVLQVVHLTVLDPSKLEVVTVAEDSRCGAGGRVTFDAAGYA
                     YVMGDGRNQSMQTFAAAAGRPTVPNCLLRMVPGEAGFEPDYSIQIPALTGGLDSMTEL
                     QPADIDSGVAFSMLKYEERIPEGLDRINFEHWGVPAYKLWRFTLGSEPRAEEVQGADF
                     SVVGFPAAAFAGKLYSSESSDGSSSTVFEIDPATNRATRKFTMDGYFAALLPLDDKR"
BASE COUNT         1959 a         3100 c         3332 g         2061 t
ORIGIN      
        1 cccggacgcg agaccctccg cagaccgtat catgcggatg gccaattctc tgcggaggag
       61 agattctggg gaacatgcca gctcgttgtc ggtgcagttg acgccgtcgc caccggcaac
      121 gcgaactcgg gcccagacga ccagcatcga accgattggt actctgctca ctcgagctct
      181 gctcactcga aggcttcccg caaaaagctg gctcggtgcg acgctgttgg cgggcggcct
      241 tgttctagcg ggagtgctgt tgcttcgctg gtttcagcat gaatcagggc cggtgttgca
      301 cgagaacctt cacgccgcgc gtacgggcca gagcgaagtg gagaagagcc cgcttcgcca
      361 atctgcgcca cctgcgagca cgggatcgat gccccagccg gaaatcagca ttccaacgac
      421 cgggccaacg gcatcagccg tcggtgcccc cgtcccaaaa cctccccgac ccgcgcgaag
      481 agggccgctg gctcctaagc tcgagtcatc gccagcggcg cctctcccct ccgcttcgtc
      541 tctcgcagag cccgagttgc ctccgagccc ggggctccga acgaagcttc gacccttgga
      601 cgataacccg tttctatcgc catccgtcga tggcgcgaac cgaccttgaa aatccgttca
      661 atcgaggcag gcgtcgcgta ctgcgctgcc gatgccggcg aagcgactga cgaggctagt
      721 cggggcgcga ttgccgttgg tgacgaccgc gatcgcgcag cgtgtgactg ggtcggccca
      781 cgcgagtacg ctgaagttgc cggcgtggcc aaagcacgaa ctcgttcgcc accagccgta
      841 cggatgaggc cagaaccaac ccatgccgaa gccgcgtccg agtactagcg gcagccgaac
      901 ggtgcggtcg atgcctcgag tctgacgcgt gatgtacttg gtgagtacgg attctccaat
      961 tagccgatgg ccgctgagag ttggggtacc gtcgagcagc caggcgtaga aacgtgccaa
     1021 cgaacgagcg gttccgatgg tgcctgcccc gggaagtatg gcggtgagct gttcgacgga
     1081 gttttgggcc tcttcgaagc caaccatctg ttctccgccc aacacgggcg gcttctcatc
     1141 cacccagtag gaccttgcta tggtgccgac ctcggcgcgg gggagaccaa gccgcaagtc
     1201 ctcgagcccc gcgggggcga gtacatcttg ctcgaggaat cgttcgatcg gcttggcgga
     1261 gatgcggcgc acgagctctc ccaaaatcca gccgaagccc agaggttgat acgctagggt
     1321 gccgcgaggg aacgccggtt ccgtggccgc gatcctcgac atcacggaat gccaatctcc
     1381 gaagtggcgc caatcacgct cgatttcccg taagtagagc cccgagcggt gcagcaggac
     1441 gtcgagtacg gagagctcgc gtttgccggc gttcgcgaat tccggccaat aatgcgctac
     1501 cgggcgcgtc acgtcgatgg ctgcggcctg ctccagcagt gcaattgcga ttgcaaccag
     1561 tggtttgccg gcgctgaaca cgcaggaacg ttgctccggc gtgaactcct gacgtggctc
     1621 atggtcggcg tctcgataac cgcgagcaac gccgacggcc tcgtcgatga ctaggcgtcc
     1681 tcggcgacga accacgagct gacctccggg gaagacgccg gtcgcttgtt gcgcccgaaa
     1741 tctttcgatg attcgcgcca cgcgagccgg gtccattccg gctccctcga catcgattcg
     1801 ctcgcgcatg gcccgccctc ctgttgccta tcgaacagtc aatggatgcc gcgaggcttg
     1861 ggtgcggtcg cgcggcaatt cttgcatcgc ccgagtagct cgaggacgtg gtcgtcgagc
     1921 aagaactcgt aacgatccgc gatatcgcgc tgcagagcct cgagcgcctc ggagcgaaac
     1981 tcgacgatgc gtccgcagcc cgtgcaaact aggtggtcat ggtggtcttt ttcgaagatc
     2041 acttcgtaac gttgcccttc accctgggac atcagcgcgg gctgtatcaa acccgcctcg
     2101 atcaggagcg ggatggctcg atagatagtg gccatatgcg catccttggc gcccttgtcg
     2161 tcgagttcgt aacacaggtc atccaccgag aagtggccag gctgcgcgag cgcggcgcgc
     2221 gcgacctgtt cgcgtacctg cgaggctctc aaggagcgcc ctcgcacgat cgaacggaac
     2281 tgctccaaag cgcgctcgat cgcatcttcc gaaagagggc gagacttacg gctgctctgc
     2341 atgggccagc aacacatggc ccctagccct ggagcggtca agccaggcca gcattctggt
     2401 gccagtcccc gcgtgccacc cagagtttcc cacgaaattc cgcagttctc gagcctttcg
     2461 agcctcgacg cgcgagcagg aactctcgcc gaatcacgcc ccgcgcgtca gcgcacgcgg
     2521 aacagtcgtg tcgaccagcc ttcggctcgg aaagccagct cgagatcggc cttcgccgag
     2581 gctacggcat aaacgtcagt cgcagaatag tccgcggtgg agagcaagat ataggtcttg
     2641 ccatctaccg agaacgaata ctgggctccg gcattccaat cgatgttggc gagcatgctc
     2701 gccgacgtgt tgccctgccc cttggaccag aaacgccagt tcgcgcccga ggtgattttg
     2761 gaggcatcgg tttcgccggc gagctctggg cggtccgcat acagcaccga gaagaatgtg
     2821 cggccatcgt cgagcacgcg aaaagccgca ccttggtggc cgccggtcag ttcggaaaac
     2881 gtgaaggcga cgtggggcgg ctcgctcccc gcagggaccc ggaacacgca tgtatcggtc
     2941 tgacccagaa cggcggcgcc gccagcggcg tagacccaat tggagaaata gaggttaccc
     3001 tcgccgtctc gcgtcgagta gtccaagccc ggacaaggcg cgtcgagcac ctcgatcaac
     3061 gagtcgctcg tcgtgtcgaa gacgacgatt tgcgaactcg catcgaactc gaagaacgag
     3121 tcgtccgtcc agtagatggg ttgataaagg tggccagcgc ggacttgcgc ggcgcgatcc
     3181 gagtagcccg ggaacggtcg aagtgagccc cgcttctcga atttgggcaa tggcgcggta
     3241 tgggtgaccc gcatggtctg cgggttccag acaatgaatt cggacgagcc gttcagcagg
     3301 taggctttgc ttggcgaaat gaaggtgttc agaaagaatc cgagattagt gagcccataa
     3361 gccccgaagt tcacgctgcc tcgttcctcg agggcaccgc ccacgacttc gaagcgggtg
     3421 atactcaggt tctcgttgct ggcgacgaat atgctttcgt cgaacaccca aagatcggcg
     3481 agccccgcaa attcgcgcgc cgccgaaaga tcgacgttgc ccttattcag agaagacagc
     3541 agtgaaacgt aggacgtggt gccctctgcg ttgaagacga ggctccccag cgcataaagg
     3601 ctctgctcct cggcatttcc ccgagcgtca gcggtcgccg aaggctccga gctcgagccg
     3661 cacgaaacgc aagtcgcgac caaagccgag atgatcgcac gtcgaccaac gccagagtac
     3721 cgttcagtaa gcgctcgagc ggcggcgacg acgcagttcg attcaggagg accttctggg
     3781 ttcgacatcc gacgctcact agcaccgggc tcagaaaatg aaaatgatat tcaacttcat
     3841 caacggcttg acgcggcggt cgatcctgtg acatgaaatc gcgaatcagt cgcggtaaat
     3901 gcgacggtgg tgctcctgga cgttcaggac gacacagcgt ggagaacgag atgcgagtga
     3961 tgaagggtcg atcccgggaa acatcattgc taggtgggct ggtcattgct gcactcggac
     4021 cacaactggc gtgcggtagt gaggcttcgc ctcaacacgc ctcggcgggg gccaccgatg
     4081 tcgccagcgg tgggacgaag gccgagggag ggcacagctc ccggggcgga gagacgtccg
     4141 cgggcggcgc tagcgctacg tcgtcgtcgc gacctctgta tgtgctcgcc aacgaagtca
     4201 gttccgggga tgacacgatc tcgtacgtca gcgtcctgga gtcactagat atcgagaacg
     4261 tcgatctcag tcgcgccgtg gagtttcccg gaggacgcgc gacgatctcg acctactcgg
     4321 gttgggtgtt cgcggcgccc cccgattcag gcgaggtgca ccgctatcgt gtcacggagc
     4381 ggggcgctct ggaggaagac ggggtcatca gcttcttgaa ctacggattc gataccgcaa
     4441 tcctagacgc ttggggtaac acgttcgtca gcgcgaccaa agcttatcta catcatcctt
     4501 ccgagggaac cagcgtcatc tggaacccga gcctcatgcg catcgaagga gaggtcgatc
     4561 ttggcggctt cgagctcatc cgaccggagt tgggggacat caacggtggc accggcgtgg
     4621 ttcgtggcaa ccgcctcttt cgaacggtgt tttggaccaa ctgggacgaa gagcagactt
     4681 ccgcggagca gtacttggcc gtctacgata cagattcgga tcgcctgatc gcgctccacg
     4741 aggaacggcg ctgtcccggc ttgagcaacc gggtgagtgg agacgaagct ggcaatttct
     4801 acttcagcaa ttggatttac aacgtgggcg agacggtgct ccgggatgca cccgagagct
     4861 gcgtgctgag actcgccccc ggttccgaac gtctggatga cttccgattt caatattcgc
     4921 gcgttaccca agggctgcaa ggtgcgaact tcgcctatct cgcggacgcg caagcggtaa
     4981 tgagcgtatt ctatccggag cgctcgcccg tgtcggcgga cagcacgccc atcagcttgg
     5041 tttcggagcc agcctggaag gtgtggtcgg tcgacttgga gggcaaatcg aagcccgtcg
     5101 aaggcttgga ttggatgtta ggggccttta cgccggtgca cttggatggg cggtcactcc
     5161 tgttgatccc aggagaagac tggtcgattg tgtacgtgaa cgagctcgtt gacggcaagg
     5221 ccgtgccccg tttcgagatt cccggctggt cgtatcaact cctcaaagtg caatgacgga
     5281 tgcgccttcg cgtcggtgcc atcgctgcgg cgctgctctg ggcagtgacg gctcgagcgt
     5341 ccgagtcatt atccggcgct ccgacccccg ctccggatct acggccccct gcggcgtcga
     5401 gaacggaacc accagggtta gcccgagcgg ttgatccctc ggtggcaacc ccgccgcgta
     5461 cgtcgcgggg tgaacctgcg ccaaagctat ttttgagctg cgggaaagac tgcttcgagc
     5521 catatctccg gcaggagttg agttacttcg acttcacgcg agacccgtac ctcgccgaca
     5581 taacggtcgt gatcgtgcgt cagccctccg ggagcgcggg cgagcgcttc acggtgacat
     5641 tgacgggccg cggagttcgg ccaagtggag ccaagtcgtt ctcggtggcg gcgggaacac
     5701 cagtccacgc tgcgcgtcag gccttgctgc agttgattct gcggcggctg ttggaggaac
     5761 tcgcggggag cgagcacgaa acggcattta gattggagtt accgcgccgg aacggtgcgg
     5821 tgctcagcac tctcgcggac acttgggact attgggtcat ttctccagaa ttgcgtggtg
     5881 agggagaggg tggcagcggc ttctacttct tccaaggagt gggggccttg acgctgaggc
     5941 gaatcacgtc cttgtcgaag ctccgctgga ggacgtcgta tacgcgccgt tggagtggat
     6001 tcctgctcga ggatgggtct cggattcggg gcgatactca cggctgggat agccgcctaa
     6061 tctatgctcg ctcgctcggt gaacgctggg cgctgggcgg agtggtgacc ggcagaacca
     6121 gtgaattcga gaacctcgag gggcacgtgc acggtggccc gttgctagaa ttgaatctgt
     6181 tcccgtatgc ggaaaatgtc actcgccagc tgcgtttcgc gtaccagacc ggcgcgtggg
     6241 gtaattggta catcgagccc aaccgagccc gagcgatccg agaactgcgc ccgtaccacg
     6301 ccgtctcgat catcgcggat ttgaatcaac gctggggaag cgttcagtgg gtagggcagc
     6361 tcaactcgtt cgtagatcgg ccgaaggctt ttcgtctgag ctccggtacc gtcttcagct
     6421 tgcgactttt cgaaggattt tcggtgcaga ccgaggggaa gatcgcatgg gtgcgggatc
     6481 tcatcaattt acggggacgg cccatcaacg atcaggaact tctgctgtgg accgccgagc
     6541 aacccactgg ctacaccttc gaggtgaagt tcggagtgtc ctacactttt ggcagcatcc
     6601 acaacacgat cgtgaacccg cgatttgctc gcgtggatct ggatgacgag taggacgtac
     6661 gggcgcttca gtggacactg gtgagcagaa gcgcttgaca gtgggagggt ggccgtgtga
     6721 tttgaatgcg cgaatcaatc gcacataagg tcctccaatt ggttagctcc ctcttgaact
     6781 cgcgtctggg ctcggcgacg atcgcgctcg tcgcctcatt cgtgtgcgca ctgatcgtgg
     6841 ttgccggcgc gagcaagtgg tttccccctg gtgcagctgg gttggaccag attgtggtgc
     6901 cgctttcggt attcccgatc gtctgggcag ctttcgtact cgtgctgcac gtggcgcagc
     6961 atcgtgcgcg ggcctggtgc ttcgtcgcgg caatcactgc ccttcacgca gttctgatcg
     7021 ccaaggggct actcagtagt tcatgagtca tcgaatctaa ccgtaagggg aaaatcaaga
     7081 gtgatatggc gggaggggta cgattcattg ccatcatcag aagttaaagc gcggcaacgg
     7141 agggcgaacg agatttacgg acatcgagct cttccatgaa acagccagcg gcttccacca
     7201 gcacctcgaa tactccagct aacttcgaga ttggcgcgcg cggtgacacc ccgcgcaaac
     7261 gtttgtactt gctgcaccgc tggctcgggg tcgtcacggc tttcgtgggc gccctcgtat
     7321 tcttcagtgg agcgattgcg actttccaca gcgagctgga tgcttggtcg agccgcgcga
     7381 accgctatcc agacgtggcc agcctgcccg gattcaacct cgattctgcc tacttggccg
     7441 caagacgagg gatagagtcc gagctgctcg agagcactga cattgcgcag gctgcggggc
     7501 acccggtctc cttcttcttt catcgtcatg agcccgacgg gatgggaacg cgcgaggtgg
     7561 gagtcttggt ggagttggac ccggaatctc tggaggtgct gagccggagg gcgggcgacg
     7621 gtgatgatgt cactcgcgcc acgcgctggt atgcgctggg ggacttcttc attcgactgc
     7681 acctttacct gctgatgcca gagacactgg gcttgatcct cacgggtacg gtgggcttcg
     7741 cactgatttt gctaatctcg tccggcattt gggtgcatcg tccgaaccta aagcgactcg
     7801 tgcgtaggcc ccggtctgaa cgtccgcggt tccttgcggc ggatacgcat accctattgg
     7861 ggtcgtggtc actgccgtac acgctgatgg tcgcgctcac cggcgcgttc ttttccttcg
     7921 ccggcaccgt gctcgttccc gcggtggcga tcgttgcctt caacggggat caaaacgcgc
     7981 tcgtggagac gctggtgggt cacgtcgcgg tctccgagcg aagcggcacc gcctcgttgg
     8041 aagccattcg gcgcgactcc ttgcaacgct gccccgtatg tagattcgag cgagtgtccc
     8101 tcgagtcttg gggcaagcgt gaagcgagcg cgaccgtgga cttgagctct tcgagcctga
     8161 cccggcacgt cgaacgccat ctggtctacg acggccacga cggcgcattt ctccaggaaa
     8221 agccgctgct cggaacacgc ccttccttag ggaattcggt gctagcgctg atggccgatt
     8281 tgcacttcgg cacgttgttc ggcctactca cgaagctgct ctgggcggtg tcggccgtgg
     8341 ccgcgtgtgg gctcgcggcc agcggtttga tcgtgtttac gacccgctcc aaggcacctc
     8401 attcaggagg cacgctgctc gtgcgaacgc tgaccatcgt gatcttgggt ggcctgccgc
     8461 tctccagcgg ttcgtcggta tacgcttggt ttttgggcat cggactccaa ttggatgcac
     8521 cgctggggct gatggcactc gcgtttgggg tcgcggtgtt ggcgactgct ggtctgggtt
     8581 tggcacgctg gccgctccgc aaagctttgg gcgtcgcact tggaagcgcc gggctgctgt
     8641 tttccctgtc gccgtgggtg gcgctcagca ttactgattt ggatctaccg cagatatggt
     8701 ttgatccggc gctgcgcccc accctttggg tagacggcgt acttagcggg ttaggcctcg
     8761 cactgctgct cattgctgga cgcatcaacc gcatgccaag caacgcggtg cagtgctcgc
     8821 ctccagcgat cggcccggcc caggtcgccg ccggggactg agccggacgt cgcgttcgtt
     8881 gaacgaagcc ggcaatttgt cgcggtccca gtctcacgga atggcctgag ccgcgcggcg
     8941 gactcgggtg atccgattgt acgaaagacg aaaccaatga aatcgagctc gtgttcatat
     9001 tcgacgctat cgtcaaagcg cgcgcgtgtt tactcctccg ttctcgcgtt cggcgtcagc
     9061 gctgccatcg gctgttctga gtcggaggcg ggcaatcctt cgctcgctgc gggggcggcg
     9121 tcgggcggca acccgtccag cggcaagccc ggaacgggcg ggagcggggc gggtggcgag
     9181 ggtggcgcga aacaagggca ggagcctcca cgcaaggtgc tgggatcgat tgtcatcgac
     9241 gccgacggga atcgcgtcag ctacgctcag attgtggggg agctttcggg gcactttacc
     9301 aatcgcgatg gaatcgaagt cagcggcaac gccaacttcc taacgcacgg cagcgacttc
     9361 ttctacggtt tggccgaatc tcccgaatgg gtgcggtact ccaccgagaa cggcttccga
     9421 gaaacggggc gactcagctt cctcaatttc ggaatgtcct atatcgactt tgccaacgcg
     9481 attgtggacg atgaaaccgc ggtgtccgtg ctgacggagg cctacgtggc ggtggtttgg
     9541 aatccgaaga gtatgaagat aaagggcacg atcgacctcg cctatctcaa aaaggacggg
     9601 ttctccctgg aggcgttcac gacggtcgcc cacgaaggtc tcgtctatgt tccggccaag
     9661 tgggtgaact gggagactcc ggaggtgctc caagtggtgc atcttacggt gctcgatcca
     9721 tccaaactcg aggtggttac cgtcgcggag gacagccgct gcggtgcggg gggacgcgtc
     9781 acgttcgatg ccgccggtta cgcgtacgtg atgggagacg ggcgaaacca atcgatgcag
     9841 actttcgcgg ctgccgcggg acggcccacg gtgccgaatt gtctgctgcg catggtcccc
     9901 ggcgaggcag gtttcgagcc ggactactcg atccaaatcc cagcgttgac cgggggccta
     9961 gactcgatga cggagctgca accggcggac atcgactcgg gcgtcgcgtt ttcgatgctc
    10021 aagtacgagg agcgcattcc ggagggacta gaccgaatca acttcgagca ctggggcgtg
    10081 ccggcctaca aactctggcg gttcacactc ggatccgaac ctcgtgcgga agaagtgcaa
    10141 ggagcggact tttccgtcgt cggctttcct gccgcggcgt ttgccgggaa actctacagc
    10201 agcgagagca gcgacgggtc ctcctcgacg gtgttcgaga ttgatccggc gaccaaccgc
    10261 gcgacgcgca agttcacgat ggacggctac ttcgccgcgc tgctcccgtt ggatgataaa
    10321 cgatagctgc ccggagtagt ggaaccggta ggccgcgaac tagcgtcact cggccgttcc
    10381 tgcaagtcca agctccggca ctcggagctt ggaccccaat cttgcctggg gcagctcgct
    10441 caacttcccc ca
//
LOCUS       DASTKU010001031         5551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_871498, whole genome shotgun sequence.
ACCESSION   DASTKU010001031 DASTKU010000000
VERSION     DASTKU010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871498"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFQ61_36575"
     CDS             complement(<1..348)
                     /locus_tag="VFQ61_36575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9960083.1"
                     /translation="MAQDTKVRYAVIGAGNIAQVAVLPAFSNATENSSLVALISGDPV
                     KRAELAEKHGIKHTGDYGELESVLQRAEVDAVYIATPNSLHREHAERAFAAGAHVLCE
                     KPLAPSLAECQAIQ"
     gene            complement(428..832)
                     /locus_tag="VFQ61_36580"
     CDS             complement(428..832)
                     /locus_tag="VFQ61_36580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960084.1"
                     /translation="MLKQDEQVAEAQRAVEMARAELADSLSTVTENSTAALKNALHKA
                     RPVLIGVAALGATVLTVKVLKSLFGSRHSRSHWQPPAPHQPSVIGGIAKSALLSVGSA
                     VVSRMAQRAILKWTENTDASWHDVPWQESRHP"
     gene            complement(816..1403)
                     /locus_tag="VFQ61_36585"
     CDS             complement(816..1403)
                     /locus_tag="VFQ61_36585"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET9960085.1"
                     /translation="MTQAADTDQTSSSGRLVQSAMMLAKAEIKLAVAETRAVAKQAAI
                     AVALSWASLSLVQVLLVLIAALPALAAVRDWPVALLSVAPCLVITGIVGFLALQRWKR
                     VSELAGARPNRTQNDVINSNPSVGAPFSSPLAPSLAPTFPLSSTYPGTFAPSTGSAPS
                     SFPPPENLTPSETRAVRGALEGDDDARAQRNVKAG"
     gene            complement(1455..1643)
                     /locus_tag="VFQ61_36590"
     CDS             complement(1455..1643)
                     /locus_tag="VFQ61_36590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960086.1"
                     /translation="MPAIHHPTHRDPSVRNTSIPDAYTEASEFIKGRPEKAPHRPAPE
                     RAFASDEFPHDERLPQCE"
     gene            1672..3018
                     /locus_tag="VFQ61_36595"
     CDS             1672..3018
                     /locus_tag="VFQ61_36595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960087.1"
                     /translation="MDRPDFVSSSGGRGASGGTTGVGGTHTGGAHAAGNGGDASSGSP
                     SEGGASSEEGGAPAQGGSSGGSGGDTQEAEGGATLQESGGSAGVTGGSGGAAPETCDD
                     PDQTQCEGSCVNLASDANHCRVCGHSCGAGSKCSSGVCQPVLLHTSPEPLTAFDVDDA
                     SIFFSPAGVSSCPNTGCKLSPDGVSSYSPGVVGNRFVADGFMLVQLPSPSAGVPNSIF
                     SLCPTSGCTTDNQITVFTTSKYVSGADFMATGNSFYWTEINPSGRLLERCSAPAGAKC
                     ERGAWMVDATLTAAKIVAASDEALFFSATLENDTQELYSCPGTVTGCTPTPMKAPSYR
                     EAVAYGEDLYLLSFPILEQVSVGRCPRTGCASIPKPVVSVPTDASRILVDASGVYWIQ
                     GEKNIQTCPLTGCVGAPFTLAKDIAAPRFMRSQGGFIYWVNAADNTIWRVAKPASK"
     gene            complement(3053..4381)
                     /locus_tag="VFQ61_36600"
     CDS             complement(3053..4381)
                     /locus_tag="VFQ61_36600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960088.1"
                     /translation="MILLNRRTWLAASLGLGLELQVEAALGLGRRPFGGTLRLSLPWG
                     VTRLDPHELDDPLGALFAGAVFDPIFSLDARGRAYATLAQGLPEPGARGGCRVRLRGG
                     LETAAGKPLLAADLKTSLERAAKRAARALVAPLGETRLNRADPLVLEFPGAASPQHVE
                     RALANPACALVPRDFTPLAPDGSGAFAARLERGRLRLERNLNAARGPAFLDAIEITTA
                     SSLAEALRDFEASKVDVGWLGSGLHQPRPGAVPFRAGRYGWVILRTGKLARAWGAPGV
                     AQLLLDGIPAERLSHLGLDNLPRQPSEVRAWAGGPAELLVVPEAPQLALVAHSLASLL
                     SQPGHELKVTELAPTELARRRSAGDYALIVDFVRSVGMGPALTQLALLTAENPALAVK
                     PPHVDHDDARSMARQLRFGVVGELAISGSYMPAFKGLSEWQLADVVRAAE"
     gene            complement(4378..>5551)
                     /locus_tag="VFQ61_36605"
     CDS             complement(4378..>5551)
                     /locus_tag="VFQ61_36605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960089.1"
                     /translation="SRVAAELGPGARAVAGLPSEAVPPKAAPRSPTELRDADAKRRVL
                     SLRLEIGERVAVDAELSQGVRRFWDRFRASAPGVLAAAHTSRPLDAEIRSFFPPVPLV
                     VQHIDKLSPADEPSVALACGDLDGDGSIELVSVGRARIKVGRFKAGHYVVEKQAAWAQ
                     LSGVAAHPLRDPIASAQILEGHGLRVGLSDRAEGLQLDEHLGVSGRFPNRAPWPPEAC
                     TQRSGLGFLTTPQDCRPERASTGKGSLGSRKEKLVEPTSDAEPTAAIDSFAGLRLIRA
                     DGTQFVIRARRDLSGRVALQVNERELESRWPAGASLALGDLDGDGRAELLSSENTLDA
                     TQDALSVRTLTAVNDLEPVLRLPVASGINAIAVCPAAPLNFAPVVLATGDGFWVLR"
BASE COUNT          971 a         1772 c         1830 g          978 t
ORIGIN      
        1 ctgaatcgct tggcactccg cgaggctcgg cgcgagtggc ttctcgcaca acacgtgagc
       61 accagctgca aacgcacgct ccgcgtgctc ccgatgcaag ctgttcggag tcgcgatgta
      121 caccgcgtct acctcggcgc gctgcaacac ggactcgagt tcgccatagt cccccgtgtg
      181 cttgatgccg tgtttctccg cgagctcggc gcgcttgacg ggatcccccg aaatcagggc
      241 caccaagctc gagttctcgg tagcgttgct gaaggcaggc aatacggcaa cctgagcgat
      301 attgcctgcc ccaatcacgg cgtaacggac cttggtgtct tgggccatgc ccacggcgtt
      361 tgcaggtccc gcgccaagct cgacgtcaca ccgctctcga gcacacgctc tcgagcacgc
      421 ccatcactca aggatgccgc gactcttgcc aggggacgtc gtgccaactg gcatccgtgt
      481 tctccgtcca cttcaggatc gcgcgctggg ccatgcgact caccactgcg ctacctacgg
      541 aaagcaacgc cgacttcgcg attccgccga tcaccgaagg ttggtgaggc gcgggtggtt
      601 gccaatgact ccgagagtgg cgcgagccga ataggctctt caataccttc acggtcaaca
      661 ccgtcgcacc gagcgccgcc acgccgatca agacgggacg agccttatgg agcgcgtttt
      721 tcaaagcggc cgtgctgttc tcggtgacgg tactcaagct gtctgccaac tccgcgcgcg
      781 ccatctcgac agcgcgttgg gcttcagcga cttgttcatc ctgctttaac atttcgttgg
      841 gctcgagcat catcatctcc ttctaaagcg ccgcggactg cgcgcgtttc ggacggcgtc
      901 aggttctccg gtggcgggaa agaactgggc gcactgcccg tactgggcgc aaacgtgccc
      961 gggtaagtcg aagacagcgg gaaggtcggg gctagactcg gcgcaagcgg cgaggagaaa
     1021 ggcgcaccaa cggatggatt cgagttgatc acgtcattct gagtgcgatt cggacgcgct
     1081 cccgccagct cggagacccg cttccaacgc tggagcgcca aaaatcccac aatcccggtg
     1141 atcaccagac atggcgcaac ggagagcagc gccaccggcc aatcgcgaac cgccgccaaa
     1201 gctggcaaag ccgcgatcaa aaccagcagc acttgaacga ggcttaggct ggcccagctc
     1261 agggccaccg cgatggcggc ctgtttggcc acggcgcggg tctcggcgac agcgagtttg
     1321 atctctgcct tggcgagcat catcgcgctc tgaaccaggc gtcccgaaga actcgtttgg
     1381 tcggtgtctg ctgcctgagt catttgtccg gtcaggctgc agttcgcgtg ccagtcgccg
     1441 cgttgtttat ctcttcattc acactgcggc agacgctcgt catgcgggaa ttcgtccgag
     1501 gcgaaagcgc gttcgggtgc ggggcggtgt ggcgcttttt ccgggcgtcc cttgatgaat
     1561 tcggacgctt cggtgtaagc gtcggggatg ctcgtgtttc ggacgctggg atcgcggtgg
     1621 gttgggtggt ggatcgcagg catggtcgcg tccacctacg ctgggtgcac cgtggaccgg
     1681 ccagatttcg tatcgagttc cggcggccgt ggcgcttctg ggggcaccac gggtgtcggc
     1741 ggaactcaca ccggtggggc gcacgccgcc gggaacggcg gagacgccag ctctgggagt
     1801 ccctcagaag gaggcgcgag ctcggaggag ggtggcgctc ccgcccaagg cgggagttcg
     1861 ggcggttcgg gcggcgatac ccaagaagcg gagggcggcg cgaccttgca ggagtcaggc
     1921 gggtcggcgg gcgtgacggg aggctccggt ggggccgccc ccgagacgtg cgatgaccca
     1981 gatcaaacgc agtgcgaggg aagctgcgtc aacctcgcga gtgatgccaa tcactgccgt
     2041 gtctgcgggc attcctgcgg tgccggctcg aagtgctcga gcggcgtctg tcagccggtg
     2101 ctgctgcata cgagccccga gccgttgacc gcgttcgacg tagacgatgc gagcatcttc
     2161 ttctcgccgg cgggtgtctc ttcgtgtccg aacactggct gcaagctgag cccggacggg
     2221 gtgagtagtt attcgccggg cgtggtgggc aaccggttcg tggcggatgg cttcatgctg
     2281 gttcagcttc ccagcccgtc ggcgggagta ccgaacagca tcttctctct ttgccccact
     2341 tcgggctgca ccacggacaa ccagattacg gtgttcacta ccagcaagta cgtctctggc
     2401 gcggacttca tggcgacggg aaactcgttc tactggacgg aaatcaaccc cagcggcagg
     2461 ttgctagagc gttgttcggc gccagccggc gctaagtgcg agcggggcgc gtggatggtg
     2521 gacgcgaccc tgacggcggc gaagatcgtc gcggcgagcg acgaggcgtt gttcttctcg
     2581 gcgaccctcg agaacgatac gcaggagctc tactcgtgtc cgggcaccgt tactggctgc
     2641 actccgacac ccatgaaggc tccgagttac cgggaggcgg tggcgtatgg cgaagatttg
     2701 tacctcctgt cattccctat cctcgagcaa gtcagcgttg ggcgttgtcc tcgtacgggt
     2761 tgcgcctcga tcccaaagcc cgtggtgagc gtgcccaccg acgcgagccg gattctggtg
     2821 gacgcgagcg gggtgtattg gattcagggg gagaagaata tccagacttg cccattgacc
     2881 ggttgcgtcg gcgcgccttt caccctggcc aaggacatcg ccgctccgag attcatgcgg
     2941 tcgcagggcg gcttcattta ctgggtgaac gccgccgata acacgatctg gcgggtcgcc
     3001 aagccggcca gcaaatagcg acgatcccga caccacggtt cgagcggcac aatcactcgg
     3061 ccgcgcgcac gacgtctgcg agttgccatt cggaaagccc tttgaacgct ggcatatagc
     3121 tgcccgagat ggcgagctcg cccaccaccc cgaagcgcag ctggcgagcc atgctgcggg
     3181 catcatcgtg atcgacgtga ggcggtttga cggcgagggc tgggttctcc gcggtcagca
     3241 aggcaagttg agtcaaggct ggtcccatgc cgacgcttcg cacgaaatct acaatcaacg
     3301 cgtagtcgcc ggcgctgcgc cggcgagcta gctccgtcgg cgcgagctcg gtgactttga
     3361 gctcatggcc aggttgcgaa agcaatgacg ccaaggaatg tgcgaccagc gctagctgcg
     3421 gagcttccgg cacgaccaag agctctgcgg gccctcctgc ccaagcgcgg acctcggagg
     3481 gttgtcgcgg caaattgtcg aggccgaggt ggctcagccg ctccgcgggg atgccatcga
     3541 gcaacagctg cgccacgccg ggcgcgcccc aagctcgcgc gagcttaccc gtcctcagga
     3601 tcacccagcc gtaacgcccc gcgcggaacg gaaccgctcc cggacgcggt tgatgcaaac
     3661 cgctgcccaa ccagccgacg tccactttgg aggcctcgaa gtctctcaat gcttcggcaa
     3721 gcgaggacgc ggtggtgatc tcgatcgcgt ctaaaaatgc tggaccgcgg gcggcgttca
     3781 gatttctctc tagcctcagc cgacctcgct ccaaacgtgc cgcaaacgca ccgctaccgt
     3841 ctggcgcgag cggggtgaaa tctcgaggaa cgagggcaca ggccgggttc gcgagcgctc
     3901 gctccacgtg ttgcgggctc gctgcgcccg ggaactcgag caccagggga tcggcgcgat
     3961 tcaggcgagt ctcgcccaac ggggcgacta gcgcgcgcgc cgcgcgtttg gcggctcgct
     4021 ctagcgaagt cttcaggtcg gcggctaaaa ggggcttgcc agcggcggtt tcgagtccgc
     4081 ctcgcagtct cactcgacag cccccgcgcg ctcccggctc tggcaagcct tgagccaagg
     4141 tcgcgtaagc gcgcccccgg gcatcgagag agaaaattgg atcgaagacc gccccggcga
     4201 acagcgctcc caaaggatca tcgagctcgt gagggtcgag acgagtcact ccccagggta
     4261 aagaaaggcg tagcgtcccg ccgaacgggc gtcgccccag cccgagcgcc gcctcgacct
     4321 gtagctcgag acccagcccg agcgatgcgg ccaaccaggt tcgccggttc aggagaatca
     4381 tctcagaacc cagaagccgt caccagtggc gagcaccacg ggcgcaaagt tgagcggagc
     4441 ggcggggcag accgcgatcg cgttgattcc gctcgcgacg ggcaggcgca gcacgggttc
     4501 caaatcgtta accgcggtga gggtgcgcac ggagagcgcg tcttgcgtgg catcgagcgt
     4561 gttttcgctg ctcaagagct cggcgcggcc atctccgtcc aagtctccca gggccaggct
     4621 ggccccggct ggccagcggc tttcgagctc gcgttcattg acctgtaacg ctactcgtcc
     4681 actcaggtcg cgacgggcgc gaatcacgaa ctgcgtccca tctgcccgaa tcaggcgcag
     4741 cccggcaaag ctgtcgatcg ccgccgtggg ctcggcgtcg gaggtcggct ccactagttt
     4801 ttcttttcgc gagccaagac tgccttttcc ggtgctagcc cgctctggcc gacagtcctg
     4861 aggggtagtg agaaaaccga gcccggaacg ttgggtgcac gcttcgggcg gccaaggcgc
     4921 gcgattggga aagcgacccg agacgccgag gtgctcgtcc agttgcagac cttcggcgcg
     4981 gtcactcagt ccgacgcgca agccgtgacc ctccaggatc tgtgcgctcg cgatcggatc
     5041 tctgagcgga tgggccgcga cgccgctcaa ctgcgcccac gcggcttgct tttctacgac
     5101 atagtggccg gctttgaatc gacccacctt gatgcgtgcc cttccgacgc tcacgagctc
     5161 gatggagccg tcaccgtcga gatctccaca ggcgagcgcg acgctaggtt cgtcggcagg
     5221 tgatagcttg tcgatgtgct gcacgacgag gggcacgggg ggaaagaagc tgcgaatttc
     5281 cgcgtcgagc gggcggctcg tgtgggcggc ggcgagcacg cccggcgcgc tcgcgcgaaa
     5341 acgatcccaa aacctgcgca caccttggct cagctcggcg tcgacggcga cccgctcccc
     5401 aatttcgagg cgcagcgaga gcacgcgccg cttggcgtcc gcgtcgcgca gctccgttgg
     5461 cgaacgcggc gctgctttgg gaggcaccgc ttcggagggg agccccgcga cggctcgtgc
     5521 tcctggcccc agttccgctg caactcggga a
//
LOCUS       DASTKU010001032        15601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1991032, whole genome shotgun sequence.
ACCESSION   DASTKU010001032 DASTKU010000000
VERSION     DASTKU010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 15601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15601
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991032"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..2612
                     /locus_tag="VFQ61_36610"
     CDS             246..2612
                     /locus_tag="VFQ61_36610"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HET9960090.1"
                     /translation="MPIRASLDAFLVAALISLTPALATAQDKSGLVAPTALSELRAPY
                     PDGASGDADVILELLIAEDGSVASSSVKSGDAPFAEAARLASESFRFTPALRGGIPVR
                     ARIAVRLVFHEPKPEPADSKLAQAEVKPNAEVKPDAAVKPDASKGNAPDEFPARNGSS
                     PSKLANPAGTNPPSGAAPAQIDARSQEPSSAEAGDSEIVVLGEQRPELGSIHIPKQEA
                     RRVPGAFGDPFRVVEVLPGVAPVLSGLPYFYVRGAPPGSVGYSIDGVPVPMLFHVGPG
                     PSVIAPMFVERVDLYPGAYPAKYGRSAGALLAGETALPSERQRVEGQARIFDASAAFE
                     QPFAKGRGNLALGGRYSYMQALLSLVAPDYELGYWDYQARASYALTPRDRVTAFGFAG
                     VDKLRNEKRRLTLFDVAFQRLDVRWDHQYTRGRVRVGVTGSSDRVLAAPEDDGAPGTS
                     QRSRGLRLRSEWEHDLGPAARLRAGADIGFERVLGEREQVGDEYVAYPNRTDRASGAY
                     ADVLLRPLRGVEIVPGLRVDAIRSRGEEHLFVDPRLSVRARLKQGVAYLAGFGLAHQV
                     PTSAVRMPGRRPNALERSVQESIQATQGLEYTLPWGMLGRTTLFHQYVNIDTEGVHGR
                     SYGVEQFLRRDFTQALGGFISYTLARAEGTDGRNTLLSGFDRTHVLSGVLGYDFGAGF
                     RFGARAYYASGRPYRVACPTIDCGPGDPRDPPVIIRSRRMPGFFRLDLRFEKRFEFEH
                     GFWLTATAEWFNATLSREVEDVDYTSRGLYFDRQSALTLPSIGLEFGY"
     gene            complement(2655..4193)
                     /locus_tag="VFQ61_36615"
     CDS             complement(2655..4193)
                     /locus_tag="VFQ61_36615"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET9960091.1"
                     /translation="MHVLRSVVPGERLNVGREILSSVVVFLVALPLCMGIAIACGVPP
                     ALGLITGIVAGLVVGALSGAPLQVSGPAAGLVVLVWEFIREFGIERLGVAVLFAGVLQ
                     FVAGALRLGRFLRAVSPAVIEGMLAGIGVLIFASQFHVMLDAKPRGSGVANLLAIPAA
                     LGQALTPATEQLQSRSAAWVALVTLGVLIAWNRLKPRALRNLPGSLMAVVAGTLVAVL
                     FGLQVNRVQAPSNLWATVQLPSWSALLEPRVLVSAAAIAVIASAETLLSAAAVDKMHG
                     GERANFDRELAAQGVGNILCGVLGALPMTGVIVRSSANVAAGARSRLSAMLHGFWLLL
                     ATTLLVSVLKLVPVAALASILVFTGFKLVNLTAVQRLRVAGRGEVLVYAVTLLAVVAT
                     DLLTGVLLGVAASVVKLALQTTSLELTHEQPGPDRVDLRLAGTATFLQLPKLAEALNK
                     LPPGQVVHVHPEHLLFIDHACVELLKTWQQTYGASGGTGFVHWQRVRARGFPLHLLAP
                     SEDT"
     gene            complement(4336..5001)
                     /locus_tag="VFQ61_36620"
     CDS             complement(4336..5001)
                     /locus_tag="VFQ61_36620"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET9960092.1"
                     /translation="MKKIIDGVHRFQKSVFSPRKEFFQRLAHEQRPEALFVTCSDSRI
                     HPQLITQSEPGELFILRNAGNLIPAYGVTSGEAATIEFAIRELGVRHLIVCGHTGCGA
                     MKALLRIEDLPQESSVRCWLGHAESTRRVVREMCQGLDGEAMLRAAVQENVLVQIENL
                     KTHPAVRSGLARGSITIHGWMYELETGRMLEYDPEHGRFERLDQRPSAARAAIDAPRL
                     QLP"
     gene            complement(5645..5986)
                     /locus_tag="VFQ61_36625"
     CDS             complement(5645..5986)
                     /locus_tag="VFQ61_36625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960093.1"
                     /translation="MLFVAIKRKGFSWLLWIAVAAIFSSLLCAPIRANAAQLSGSAAS
                     ARENLDEEVAAVVRSTSEREVSVRRTQPRELPAVRSSRPQEFGQSPKVRPRPVRGPTC
                     PIPIRLRRLLN"
     gene            complement(6394..7005)
                     /locus_tag="VFQ61_36630"
     CDS             complement(6394..7005)
                     /locus_tag="VFQ61_36630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9960094.1"
                     /translation="MAFALLRASARTDHVSRHLALAHAIDESASELFEECGLHVDFDA
                     SHPFARAEDARFRASLEAGNLWFALPDEGAPVEAALGFIALGCVDAQPFVYQLSVRRE
                     AMRRGVGRTLLEAALTTARRLHAGSSQPATVAVTQPASVYGLWLTTWAHLPWNQAYYE
                     RFGFRVIGDQHYGRELRDLIEEEQRWLPAPEQRRVMCCPMERA"
     gene            complement(7387..8607)
                     /gene="fmdA"
                     /locus_tag="VFQ61_36635"
     CDS             complement(7387..8607)
                     /gene="fmdA"
                     /locus_tag="VFQ61_36635"
                     /EC_number="3.5.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF045496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004328 - formamidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formamidase"
                     /protein_id="HET9960095.1"
                     /translation="MDTLISVDVAAPPETQDVLHNRWHPDIPIVSWVKPGDQFRIECV
                     DWTGGQIKNDESATDIKVVDLTKVHYLSGPIGVKGAEPGDLLVVDILDVGVLKSSQWG
                     FTGIFAKENGGGFLTDHYPEARKACWDFSGIYANSRHIPGVEFAGIMHPGLIGCLPSK
                     DLLDTWNKREKALFDTNPERVPPLCALPSAPTAHLGRMKADAARAAALEAARTVPPRE
                     HGGNCDIKNLSRGSTVYFPVYVSGGGLSMGDIHFSQGDGEITFCGAIEMAGYLDIRVN
                     VIKGGMAKYGIKNPIFKPSPLEPRYSSYLIFEGISVDEAGKQHYLDVHVAYRQACLNA
                     IEYLKKFGFTGAQAYAILGTAPVEGRISGIVDIPNACATLALPTEIFSFDIKPSATAP
                     TVGISPGKDLAIVT"
     gene            complement(8697..9392)
                     /gene="urtE"
                     /locus_tag="VFQ61_36640"
     CDS             complement(8697..9392)
                     /gene="urtE"
                     /locus_tag="VFQ61_36640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HET9960096.1"
                     /translation="MAKILEVDEIRVAYGQSEVITRMSLSAPENEVLAVMGLNGMGKT
                     TLMKALIGLLPVRGGGIRLAGKDLTRAPAFERVRAGLGYVPQGRMVFPTLTVEENLQS
                     GLAPKERKVPEEIYKFFPVLREMRKRRAGNLSGGQQQQLAIGRALASRPKVLLLDEPT
                     EGIQPSIIKELATTLKQIRAEQGLTIVVSEQVLSFALAVADRVLVMARGAIVQESSRS
                     ALDEASLGKLLSI"
     gene            complement(9406..10143)
                     /gene="urtD"
                     /locus_tag="VFQ61_36645"
     CDS             complement(9406..10143)
                     /gene="urtD"
                     /locus_tag="VFQ61_36645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HET9960097.1"
                     /translation="MNNFLLEVEGLTVVFDGFKAVDDLNLYIDNNEVRAIIGPNGAGK
                     TTLLDLISGRTQPTQGLVRFAGSNLTGLREHEIVRAGIGRKFQTPSVYESLTVFENLE
                     MSYPGSRSFWSAWRFQRTPEVVRKVEEIAEIVYLKDHLNDPAEILSHGQKQWLEIGAL
                     LIQDPKLMMLDEPVAGMSVPEREKTAELLIELGKGRSVIVVEHDMDFVRQIAHRVTVL
                     HQGRLLVEGKMEQVQNDPRVVEVYLGH"
     gene            complement(10140..11294)
                     /gene="urtC"
                     /locus_tag="VFQ61_36650"
     CDS             complement(10140..11294)
                     /gene="urtC"
                     /locus_tag="VFQ61_36650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HET9960098.1"
                     /translation="MLAIRSKHGMYFALLALVLLVVFPLALDTFRLNLMGKYLTYAFV
                     AIGIAICWGYGGILSLGQGVFFGLGGYCMAMFLKLEASDPASTSIQSTPGIPDFMDWN
                     QLTALPTFWIPFQSFPFTLLAILLVPAGLAWLIGFAMFKRRVGGVYFAIITQAIAAIL
                     SILIIGRQGYTGGVNGITDLKTMLGWDIRTDSARLILYFANAILLLAGVALSLSLVHS
                     KLGRILVAVRDREERVRFTGYDVANMKTFVFVFAAVYAALGGAMFTLQVGFMSPSFVG
                     IVPSIEMVIATAVGGRTSVFGAVYGSILVNVGKTYFSESFPELWLLLIGSLFIAVVML
                     FPNGLAGIDSKRLLSQFRKLAGPLRQALQSRLHKAPPSKAMPSPAGGGKP"
     gene            complement(11298..12206)
                     /gene="urtB"
                     /locus_tag="VFQ61_36655"
     CDS             complement(11298..12206)
                     /gene="urtB"
                     /locus_tag="VFQ61_36655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HET9960099.1"
                     /translation="MNVSFPIWAMQAFNGLSIFCVLLLMALGLAIIFGQMGVINMAHG
                     EFLTLGAYTTFLLSEACTRFAPGMRNWYFLVSIVASFALASALGYLLEHVMIRHLYKR
                     PLDTLLATWGLSLILQQVYRSTFGAREVSATLPDWLMGSVKPTPNIDIPINGLVVIVL
                     TLCLTALVFFGLWYTRLGVRIRATMQNRAMASAAGIDTARIDRFTFAFGCGLAGVAGA
                     AFTTIGSTGPTSGSLYIVDTFLVVVFGGAESLLGTIASSFFIAQTQATTEFFMTGSMA
                     KVLTLSLVIFILMLRPQGLFTIKVRK"
     gene            complement(12264..13529)
                     /gene="urtA"
                     /locus_tag="VFQ61_36660"
     CDS             complement(12264..13529)
                     /gene="urtA"
                     /locus_tag="VFQ61_36660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HET9960100.1"
                     /translation="MSKTALISSLNYRFFALLLALGAALGGCQKAKGSTPSAGTEKPG
                     LDSAKAAIAVTPTEVTVGQLHSATGTMAISETGSIQAERLAIDQINGMGGILGRKIKI
                     IQEDGASDWPTFAEKAKKLLVKDRVASVFGCWTSASRKAVLPVFEKENGLLYYPTFYE
                     GLEQSKNVFYTGQEATQQILAGLDWLYKTKNAKTFFLIGSDYIWPRTSNKIARKHIEN
                     VLKGKVVGEEYYPLGHTQFGSLINKIKAEKPDAVYAIVVGGSNVSFYKQLKAAGVTAE
                     NQTLLTISVTEDELLGIGGENMVGFYAAMKYFQSLDNENNKKFVAAFKEKYGPKSVIG
                     DVTQAGYLGPWLWKAAVEKANSFEVDKVVAASPGIELTTAPEGYVKVHENHHLWSKLR
                     IGQAQMDGQYKVVYESDLIEPNPFPKGYQ"
     gene            13899..15470
                     /locus_tag="VFQ61_36665"
     CDS             13899..15470
                     /locus_tag="VFQ61_36665"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myxococcus cysteine-rich repeat containing
                     protein"
                     /protein_id="HET9960101.1"
                     /translation="MTHRADSPRRKLATRHSASRTWPAFLSLAAVWACGRSDLKDSLY
                     DGPWTIEEGGTGGLSQIAGGTPSGGTVSAGGAGATSFGGAGNAPWGGTVGDGGSSGQT
                     TNASGAGSGFGGIGGEGGAAVGGFAETGGFAPAGGTVAFGGSTAFGGSTAFGGTIGVG
                     GVTLFGGNTARGGTTSMGGTTAAGGVPVSGGTSTAGGVTAAGGTSGIGGAGSGGRTEL
                     GGASSTGGVNLGGATSGGRGGTTSMGGTTSMGGTTATGGAGRGGATASGGTSASGGLT
                     GTGGTTQQPRCGDGRVQAPEACDLGVKNADRPALELRQGALIRAVLPIARPATASAFY
                     AYSSGSSHTGFEALNTSSFFWYRDSATGALSLIQHHGVDLDSTGVRQPSSALSVDITG
                     LPTKTLVVISDDAGELVKTGPNSALGSFKFNRNSDGGILSNFDFPSAFSVEVNVSVDT
                     GTTLLRFIDDSGVAIPLNLDLPFDLIAYASPSTCRTTCTIPRCGDGILDGGETCDDGN
                     TVTGDGCSADCRTLR"
BASE COUNT         3019 a         4806 c         4870 g         2906 t
ORIGIN      
        1 ccgttttcaa tgccgctttc gcggccagtt caatgccacc tttgcggcca caccaatccc
       61 gaatcgcggg gaggataacg tggctcgttt cgaactgtca attcgctttt ggtcgaagct
      121 gatctgggcg gggcgatggt cgtcactctg ccccgacgac ggttcacgcc cagcaaccgc
      181 gggctcgctc ctcatcacac ccagcggacg ggtgtgatcg gatggagtag ggtgcgcgct
      241 ccagaatgcc gatacgcgcc tcactcgatg ctttcctcgt tgcggctctc atctcgctca
      301 cgccggcact ggcgacggcg caagacaagt cgggtttggt ggctcccacc gcgctgagtg
      361 agttgcgagc accgtaccca gacggagcgt ccggggacgc agacgtgatt ttggagctcc
      421 tgattgccga agacgggagt gtggcttcga gcagcgtgaa gagcggagat gcgccgttcg
      481 ccgaggcagc gcgtctcgcg tcggagtctt ttcgcttcac gcccgcgctg cgtggcggca
      541 ttccggttcg ggcacgaatc gcggtgcgtc tcgtgtttca cgagccaaag ccagagcccg
      601 cggactcgaa gctcgcgcaa gccgaggtga aaccaaatgc agaggtgaaa ccagatgcag
      661 cggtcaaacc ggatgcatcg aaggggaatg ccccggacga gtttccggcg aggaatggct
      721 catccccgag taaactcgcg aacccagctg gaaccaaccc tccctccggc gcagccccgg
      781 cccagattga tgctcgaagc caagagccga gctcagccga ggcgggggac tccgagattg
      841 tcgtgcttgg cgagcagcgc cccgagctcg gctcgattca tattcccaag caggaggcgc
      901 gccgggttcc gggggcgttt ggcgatccat ttcgcgtggt cgaggtgttg cccggggtag
      961 cgccagttct gagcggattg ccttacttct acgtgcgcgg cgcgcctccc gggagtgtcg
     1021 gatactcgat tgacggcgtg ccggttccga tgctgttcca cgtagggcct gggccctcgg
     1081 tgatcgcgcc gatgttcgtg gagcgggtcg atctctaccc aggcgcttat ccagccaagt
     1141 acggccgctc tgcgggcgcg ctcttggcgg gcgagacggc gctgccgagt gaacgccaaa
     1201 gggtcgaggg gcaggcgcgc atattcgacg cgagcgccgc cttcgaacaa cccttcgcca
     1261 agggtcgcgg caatctggcg ctcggcggtc gctacagcta catgcaggcc ctgctctcgc
     1321 tggtcgcgcc ggactacgag ctcgggtatt gggactatca ggcacgcgcc agctatgcgc
     1381 tgacaccacg tgatcgcgta accgcgttcg gattcgcggg ggtcgacaaa ctgcgcaacg
     1441 agaagcgccg gctcacgttg ttcgacgtcg ccttccagcg tttggacgtg cgctgggatc
     1501 accaatacac gaggggccgc gtgcgcgttg gagtgaccgg gagttcggac cgcgtgctcg
     1561 cggcaccgga ggatgacggc gctcccggca cgtcccagcg cagccggggg ctgcgcttac
     1621 gttcggagtg ggagcacgac ctcggccccg cggcgcgact ccgggcgggg gcggacatcg
     1681 gattcgagcg ggtgctgggt gaacgcgagc aagtaggaga tgaatacgtc gcctacccga
     1741 atcgaacgga tcgggcgtcg ggtgcctatg cggacgtgct gttgcgcccg ctgcgggggg
     1801 tcgaaatcgt gcctgggtta cgcgtcgacg cgatccgctc acgaggcgaa gagcacctct
     1861 tcgtggatcc tcgactttcc gtgcgcgcgc gtctgaagca aggggttgcc tacttggccg
     1921 gcttcgggct tgcacaccaa gtgcctacct cggcggtcag gatgccaggg cgcaggccca
     1981 acgccttgga gcgctcggtt caggagtcca tccaagccac ccaggggctc gagtacactc
     2041 tgccctgggg catgctcggt cggaccacgc tctttcatca gtacgtaaat atcgatacag
     2101 agggcgtgca cgggcgaagc tatggagtgg agcagttctt gcggcgcgac ttcacccagg
     2161 ccctgggtgg cttcatttct tatacactgg cacgcgccga ggggacggac ggcaggaata
     2221 cgcttctttc aggcttcgac cgcactcatg tgctctcggg cgtgctcggc tacgacttcg
     2281 gggccggctt tcgtttcggg gcgagggctt attacgcttc gggacgcccg taccgcgtcg
     2341 cctgtcccac catcgattgt ggcccgggag acccgcgaga cccgcccgtc atcattcgca
     2401 gtcgcagaat gccaggcttc ttccgattgg atctgcgttt cgagaagcgc ttcgagttcg
     2461 agcatggctt ctggctcacg gccacggcgg agtggttcaa cgccacgctc tcgagagaag
     2521 tggaagacgt ggactacacg agccgcggct tgtacttcga tcggcagagc gctttgacgc
     2581 tgccgagtat cggactcgag ttcggatact gagctcgagc ccgattcatg agcatgcgcg
     2641 ggaggcgggg ggctttaggt gtcttcgctc ggggcgagca gatgcaatgg aaagcctctc
     2701 gccctcaccc gctgccagtg cacgaagccc gttcctccgg aagcgccgta ggtctgctgc
     2761 caagtcttca acagctcgac gcaggcgtga tcgatgaaca gcaagtgctc ggggtgaacg
     2821 tgcacgactt gccccggcgg gagcttgttc aaagcttccg ccagcttggg gagttgcagg
     2881 aaagtggcag tgccggccaa acgcaggtcg actcgatcgg gccccggctg ctcgtgggtg
     2941 agctctaggc tcgtggtctg gagggccagc ttgacgaccg aggcggccac gccgagcagc
     3001 acccccgtca acaggtccgt ggccacgacc gcgagcaacg tgacggcata gacgagcacc
     3061 tcgccacgcc cggctacccg caagcgttgg acggcggtga ggttcacgag cttgaagccg
     3121 gtaaacacca agatggacgc gagcgctgcg accggcacga gcttcaatac cgagaccaac
     3181 agggttgtgg ccagcagcaa ccaaaagcca tgtagcatcg cggagagccg actgcgcgcg
     3241 cccgcggcta cgtttgctga gcttcgtacg attacgcccg tcatgggcag tgcaccgagt
     3301 acgccgcaca agatgttgcc gacgccctgg gccgccagtt cacgatcgaa attggcccgt
     3361 tcgcccccgt gcatcttatc gaccgcggcc gccgacagca gcgtctccgc actcgcgatg
     3421 actgcaatcg ccgcggcgga gaccaacact cgtggctcga gtaacgcgct ccagctcggg
     3481 agctgcacgg tggcccacaa attcgacgga gcctgaactc gattgacttg taaaccaaac
     3541 agcaccgcca ctagcgttcc tgcgaccacc gccatcaacg agcccggcag attgcggagc
     3601 gcacgcggct tgagtcgatt ccacgcaatc aggaccccca gtgtgaccag cgcgacccag
     3661 gctgcggagc gggactgcag ctgttcggtc gcgggagtga gcgcctggcc gagcgcggcc
     3721 gggatcgcga gcagattggc tactccgctg ccacgcggct tcgcgtccag catgacgtgg
     3781 aattgactcg cgaagatcaa aaccccaatc ccagccaaca tgccttcgat gacggcaggg
     3841 gagaccgcgc gtagaaaacg gcccaggcgc agtgcaccgg ccacgaactg cagaaccccg
     3901 gcgaacagca cggcgacccc tagtcgttcg atcccgaact cgcggatgaa ctcccacacc
     3961 agcacgacta gtcccgcggc ggggccgctc acttgcagtg gcgcgcccga cagcgcgcct
     4021 actaccagcc ccgcaacgat gcccgtaatc aagcccaatg cgggcggcac cccgcacgcg
     4081 atggcgatgc ccatacagag cggcagcgcg accaggaaca ccacgacgga agacagtatt
     4141 tctcgtccta cgttgagccg ctccccagga acgaccgaac gtaggacatg caatggaaag
     4201 aaaaggcgca ttagagcctc cagagtcaga gcgaaagttg cagaaccaag ctcgaggacg
     4261 cctaggcgtc ccgagctctg gcgtgattga agtcgcgaag cgagcggcgc cccgctcagg
     4321 gcaattccgg gcaattcagg gcaattggag gcgcggtgca tcgatcgcag cgcgtgctgc
     4381 cgacggccgc tgatcgagcc gctcgaaacg accgtgctct ggatcgtatt ccagcatgcg
     4441 cccggtctcg agctcgtaca tccaaccgtg gatagtgatc gagcctcgcg ccagtcccga
     4501 gcgcactgcg gggtgcgtct tcagattctc gatctgtacg agcacatttt cctgtaccgc
     4561 cgctctcaac atggcctcac cgtccaaacc ctggcacatt tcgcgcacca ctcggcgcgt
     4621 ggactcggcg tgcccgagcc agcaccgaac cgaggattcc tggggcaaat cttcgattcg
     4681 cagcaatgct ttcatcgcgc cgcaaccggt gtggccgcat acgatcaaat gccgcacccc
     4741 gagctcccgg atcgcaaact cgatggttgc ggcctcgccg gaggtcacgc cgtaagcggg
     4801 aattagattg ccggcgttcc tgaggatgaa gagctctccc ggctccgatt gcgtaatcag
     4861 ctgcgggtga atgcggctat cggagcaagt gacgaacaaa gcttccgggc gctgttcgtg
     4921 ggccagacgc tggaagaact ctttgcgtgg tgagaagaca ctcttttgga atcgatgtac
     4981 gccgtcgatg atctttttca tgatatgtct cgcattcgaa agttggattc agcctggagt
     5041 tcacgccggc gacggacgaa gtcggtaaag gccaaagcga gaccgcttca gattggctgt
     5101 cggcgcacgc agcccccgca gggctatggc gagcacgtcg tcatccaaac tgacgctgag
     5161 actgcgaggg attaccggct tcgggctgga ggcgcgaatt caggtttgga gagcaggcac
     5221 accagttccg tgtcctcgct tggtcaaaag cgaggactga ctggccttag aaatggagtt
     5281 tggcctctcg ctaccagacg ctcgtgagcg aagagggtgc gggtgaacga agcacgctta
     5341 tgcgtggagg agagtgcgat cgagcgcgcg gatcttacgg agcgctccgc tcccgtgctc
     5401 acttacggct gaccgccacg gaaacgtctt ggcgcgtgga gctgccactg cgggcagacg
     5461 aagattgacg tcacgtcccc gagctgcacc gtagtcacgc aaatatctaa tttagcagta
     5521 gtcgagttga ctcggggcta ctagccagtt gccgacgcgc cactgcactc gtgacattgc
     5581 actcgtgaca ttgcactcgt gacattgcac tcgtgacatt gcagtggcgg aagcggcttt
     5641 cggctcaatt caataatcta cgcaaccgaa tcggaattgg acacgttgga ccacgcacgg
     5701 gtcgcggcct aaccttggga ctttgaccga actcctgcgg gcgagagcta cgcaccgcgg
     5761 gtagctcccg cggctgcgtg cgacgaacgc ttacctcgcg ttcgctggta gagcgaacga
     5821 cggccgcaac ctcttcatcc agattctcgc gcgcactggc cgcgcttccg gagagctggg
     5881 ccgcgttcgc gcgaatgggc gcgcacaaaa gggacgagaa gatggccgcc acggcgatcc
     5941 aaagcagcca agaaaaaccc ttcctcttga tagcgacgaa caacatcggc cctcagcctg
     6001 aggagtggat ccgaaaccac gggcccactc atcggctcaa ttgcgttgcg cggggactat
     6061 cccaaaagaa gagcgcccac gcaagcgccc agttggtaac gagggcacga gttgtcacac
     6121 cgcgcttgca gctcgccatt ggccgaccgt ttggcgagtg ctggctctcg cgagtaccgc
     6181 tgctcgttgc tagatggcta gtcctgaatg gctactattg gggagcacga gccatccgac
     6241 aaaactggag gctctatcgt cacgcggatc ccagtgctca acatggatga cgttgttttg
     6301 cccgtctatc gcctccattg tgggccgcga ggcagaatgg cgcgcactct ctcgcgtggg
     6361 ccatcatttg tggaacctgt tgcatagccc tctctaagct cgttccatag ggcagcacat
     6421 caccctgcgc tgttccggtg ccgggagcca gcgctgctcc tcttcaatca agtctcgaag
     6481 ttcacgccca tagtgttggt ctccaatcac cctgaacccg aacctctcgt agtaagcctg
     6541 gttccagggc aggtgggccc aagtcgtcag ccacaagccg tagacgctcg ctggttgggt
     6601 gacggctact gtcgccggct ggctagagcc cgcatgcagg cggcgtgcag tagtcagtgc
     6661 agcctcgagc agcgtgcgac ccacgccccg gcgcatcgcc tcgcggcgta ccgacaactg
     6721 ataaacgaac ggctgtgcgt cgacgcagcc gagcgcgatg aatcccaggg ccgcctccac
     6781 cggagcgcct tcgtccggca gtgcgaacca caggttgccg gcctccaaac ttgcgcgaaa
     6841 gcgagcatcc tctgctctcg caaaaggatg tgaagcatcg aaatctacgt gcaatccaca
     6901 ctcttcgaac aactctgacg ctgactcgtc gatcgcatgg gcaagtgcca agtgccgaga
     6961 tacgtgatcc gtgcgcgcag aggcgcggag gagggcgaac gccatggcct agcgtgcacc
     7021 ctcctcccac gtggagctag cgcgaatctc ggacggattc gttgggggct aggcgtgttc
     7081 gattacccag ggcctcggcc cagtatacgt gcgggcgacg gcaggttccg gacggctcgg
     7141 tgttcccggc gagacgcgcg gttcgtgccg cgccctctcg ttgcgatccc gggcgcgaag
     7201 ttgtgagcga gagagcacac cgagctgcgg agcactcagg atccgtggcg acgaggcctg
     7261 acattccggg cacgcgctgg gctcccgcgc ctcctgcatc gccctgagcc gttcgaacac
     7321 cccgtggctc gcacactcgt actcatagag aggcatgagt catgatctcc aatcagctgg
     7381 tgcgtatcag gtcacgatcg ccaggtcttt gcctggtgaa attccgaccg tcggcgccgt
     7441 ggcgctcggt ttgatatcga aggagaagat ttcggtcggt aacgccaagg tggcgcaggc
     7501 attcgggatg tccacgatac cactgattcg accctcgacc ggggcggtgc cgaggattgc
     7561 gtaggcttgc gcgcccgtga aaccaaattt cttcaagtac tcgatggcgt tcaggcaagc
     7621 ttgacggtac gcgacatgga cgtcgaggta gtgctgcttg ccggcttcgt cgaccgagat
     7681 gccttcgaag atgagatacg acgaatagcg gggctcgagc gggctgggct tgaaaatggg
     7741 attcttgatt ccgtacttcg ccatgccccc cttgatgacg ttcacccgga tgtctaggta
     7801 gccggccatc tcgatggcgc cgcagaacgt gatttcgccg tccccttggg agaagtgaat
     7861 atctcccatg gagagcccac caccggatac gtacaccggg aagtagacgg tggagccgcg
     7921 ggacaggttc ttgatgtcgc aattgccacc gtgctcccga gggggcaccg tacgggccgc
     7981 ttcgagtgcc gccgcgcgcg ccgcgtctgc tttcatccgg ccgaggtgcg cggttggcgc
     8041 cgagggcagc gcgcaaagtg gcgggacgcg ctcgggattg gtatcgaaca gcgccttttc
     8101 ccgcttgttc caggtatcta gtaagtcctt ggagggcaga cacccgatca aacccgggtg
     8161 catgataccc gcaaactcca cacctgggat atgacgcgaa ttggcgtaga tgccgctgaa
     8221 gtcccaacag gctttgcgcg cttcgggata gtggtcggtt aggaacccgc ctccgttctc
     8281 tttcgcgaag attcccgtga agccccactg ggaagacttc aggacgccaa cgtccagaat
     8341 gtcgaccacc agcagatcgc ccggctccgc gccctttacg ccgatcgggc cgctcaggta
     8401 gtgcaccttc gtcagatcga cgaccttgat gtcggtggcg ctctcgtcgt tcttgatttg
     8461 tccccctgtc caatcaacgc actcgatgcg gaactgatca ccaggcttca cccaagacac
     8521 gatgggaata tcggggtgcc agcggttgtg caaaacgtct tgagtctctg gtggggccgc
     8581 aacgtcgacg ctgatcagtg tatccatgtt gtatcgtctc ctgggtgggt tcggaaggca
     8641 gactggtaag gtgcgcacgc aatctcgcga gcaccgaaat cagatcgttc acggaatcaa
     8701 atagagagca gcttgccgag tgaggcttca tccagcgcgc tgcgtgagct ctcctgcacg
     8761 atcgcgccgc gcgccatcac cagcacgcgg tccgcgaccg ccagcgcaaa actcagcacc
     8821 tgttccgaga ctacgatcgt tagcccctgt tcggctcgaa tctgcttcaa ggtggtggcc
     8881 agctccttga tgatagaggg ctgaatccct tcggtgggct cgtccaacag cagcaccttg
     8941 gggcgactcg cgagcgcgcg tccaatcgcg agctgctgct gctgaccgcc cgacaggttg
     9001 cccgcgcgcc gcttgcgcat ctcgcgcaaa accgggaaga acttgtaaat ctcctcggga
     9061 actttgcgtt ccttcggcgc gagcccactc tgcaggttct cttccacggt cagagttgga
     9121 aacaccatgc gtccttgagg cacgtaaccg agcccggcgc gtacccgctc gaaggcgggc
     9181 gcgcgcgtca agtccttgcc tgctaggcgg atccctccac cccgcacggg cagcaagccg
     9241 atcaacgcct tcatcagggt cgtcttgccc atgccgttca ggcccatgac cgcaagcact
     9301 tcgttctccg gtgcggacag agacatgcga gtgatgacct cgctttgacc gtatgcgacg
     9361 cggatctcat cgacttccaa tatcttcgcc atgggtctct ccgtactagt ggccgaggta
     9421 cacttcgacg acccgcgggt cgttctgcac ttgctccatt ttgccctcca ccaacaagcg
     9481 accttggtgc aagaccgtga ctcggtgtgc gatttggcgt acgaagtcca tgtcgtgctc
     9541 cacgacgatc acggagcgcc ctttgccgag ctcgattagg agctcggccg tcttctcgcg
     9601 ctcgggcacg ctcatcccgg cgaccggctc atccagcatc atgagcttcg gatcctgaat
     9661 cagcagcgca ccgatctcga gccattgctt ttgcccgtga ctcaggatct ccgcgggatc
     9721 gttcaggtga tccttgagat agacgatttc ggcaatctct tcgactttgc gcaccacctc
     9781 gggcgtgcgc tggaaacgcc aggccgacca gaagctgcgt gagcccgggt aggacatctc
     9841 gaggttttcg aatacggtca gtgactcgta tacggacgga gtttggaatt tgcgaccaat
     9901 tcccgcgcgc acgatctcgt gctcgcgcag ccccgtcagg ttgcttccgg cgaagcgcac
     9961 cagcccttgg gtgggctggg tgcgaccgga aatcaaatcg agcagggtgg tcttcccggc
    10021 gccgttgggg ccgatgatgg cgcgaacttc gttgttgtcg atatagaggt tgaggtcatc
    10081 gacggccttg aacccgtcga acactaccgt caagccttcg acctcgagca agaagttgtt
    10141 catggtttgc cgcctccggc gggtgagggc atggccttgc ttgggggggc cttgtgtaag
    10201 cggctctgca gcgcttgacg cagaggtccg gccagcttgc gaaactgaga gagcagtcgt
    10261 ttgctgtcga tgcctgcgag cccattgggg aacagcatga cgacggcgat gaacagtgag
    10321 ccaatcaaga gcagccacag ctcgggaaag ctctccgaga agtaggtctt gccgacattg
    10381 acgaggatac tgccgtatac cgcgccgaag accgaggtgc gcccgcccac cgcggtggcg
    10441 attaccatct cgatcgaagg cacgatgccg acgaaggagg gggacatgaa gccgacctgg
    10501 agcgtgaaca tcgcgccacc caatgccgcg tacacggcgg cgaacacgaa aacgaacgtc
    10561 ttcatgttgg ctacgtcata gccggtgaac cgcacgcgct cttcgcgatc acgcaccgcg
    10621 accaagatcc ggccgagttt gctgtgcacg agtgacaacg agagggcaac ccccgccagt
    10681 agtaggatgg cattggcgaa gtagaggatc agccttgccg agtcggtgcg gatgtcccag
    10741 cccagcatgg tcttcagatc cgtaatgcca ttcacgcctc cggtgtaacc ctgacgaccg
    10801 atgatcaaga tcgagaggat ggcggcgatg gcttgcgtga taatcgcgaa gtagaccccc
    10861 ccgactcggc gcttgaacat cgcgaagcca atcaaccagg cgagccccgc cggtacgagg
    10921 agaatcgcca gcagcgtgaa cgggaagctc tgaaacggaa tccagaacgt ggggagtgcg
    10981 gtgagctgat tccaatccat gaagtccggg atccccggcg tagattgaat ggaagtgctc
    11041 gccgggtcgg aggcttcgag cttcaggaac atggccatgc agtagccgcc gagaccgaag
    11101 aacacgcctt ggcccaaact cagaatgcct ccgtaacccc agcagatggc gatcccgatc
    11161 gcgacgaacg cataggtcag gtacttgccc atcaagttca gccggaacgt gtccaaggcg
    11221 agcgggaaca ccaccaagag caccagcgcc aagagcgcga agtacatgcc gtgtttggat
    11281 cgaatcgcga gcatcgctca cttcctcact ttgatggtga aaagaccctg cgggcgcagc
    11341 atcaggatga agatgacgag ggacagggta agcactttgg ccatcgaccc tgtcatgaag
    11401 aactcggtag tagcctgggt ctgggcgatg aagaacgaag acgcgatggt gccgagcagg
    11461 ctctccgcac cgccgaacac gacgaccaag aacgtgtcca cgatatagag tgagccactc
    11521 gtcggacccg tcgacccgat ggtggtgaac gccgcgcccg ccacgcccgc aaggccgcaa
    11581 ccgaacgcga acgtaaacct atcgattcgg gccgtatcga tgccagcggc ggatgccatg
    11641 gcccgatttt gcatggtggc gcggatgcga acgccgagcc gcgtatacca taggccaaag
    11701 aacaccagcg cggtcagaca caaggtcagc acgatcacga ctagcccgtt gatggggatg
    11761 tctatattcg gggtgggctt gaccgagccc atcagccaat ctggtaacgt cgcgctcacc
    11821 tcgcgtgccc cgaaggtcga gcggtagact tgctggagta tcaagctcag cccccacgtg
    11881 gccagcagcg tatccagagg gcgtttgtac aggtgccgaa tcatcacgtg ttcgagcaaa
    11941 taaccgagcg ccgaggcaag cgcgaaggac gccacgatcg acaccagaaa gtaccaattg
    12001 cgcattcccg gtgcgaagcg cgtacaagcc tccgagagca gaaaggtcgt gtacgcgcca
    12061 agcgtcagga actcgccgtg cgccatgttg atcaccccca tttgcccgaa gatgatggcc
    12121 aatccgagcg ccatcagcag caatacgcag aagatgctca agccgttgaa ggcttgcatc
    12181 gcccagatcg gaaatgacac attcatggcc ggctctttcg gttgtgggct aactagctag
    12241 gaggacagaa tcacacgatc cactcactga taacccttgg ggaagggatt gggctcgatc
    12301 agatcggact cgtaaaccac tttgtactga ccatccatct gtgcttgtcc gatgcggagc
    12361 ttgctccaga gatggtggtt ctcgtggacc ttgacgtaac cctccggcgc cgtcgtcaac
    12421 tcgatgccag gggaggcggc tacgactttg tcgacttcga agctgttggc cttttcgacg
    12481 gcggccttcc atagccaagg tccgaggtaa cccgcctggg tgacgtcgcc gatcaccgac
    12541 ttgggaccgt atttctcctt gaaggcggcc acgaacttct tgttgttctc gttgtccaag
    12601 ctctggaagt acttcatggc cgcgtagaaa ccgaccatgt tttctccgcc gatgccgagc
    12661 agctcgtctt ccgtgaccga gatcgtcaac agcgtctggt tctctgcggt cacgcccgcg
    12721 gccttgagtt gcttgtaaaa ggacacgttg ctgccgccga ccacgatcgc ataaaccgcg
    12781 tccggtttct cggccttgat tttattgatc aaggaaccga actgggtatg ccccagtgga
    12841 tagtactcct cgccgacgac cttgcctttc agcacgttct cgatgtgctt tctcgcaatc
    12901 ttgttcgagg tgcgcggcca gatgtaatcc gagccaatca ggaagaaggt cttggcgttt
    12961 ttggttttgt acaaccaatc taggccggcc aagatctgct gcgtggcctc ctggcccgta
    13021 tagaacacgt tcttggattg ctcgagtcct tcgtagaagg tcggatagta gaggaggccg
    13081 ttttcctttt cgaataccgg taacaccgct ttgcgggagg cggaggtcca acagccaaac
    13141 accgaagcga cccggtcttt gaccagcagc ttcttggctt tctcggcgaa ggtcggccaa
    13201 tcactggcgc cgtcctcttg gatgattttg attttgcgac ccaagatgcc gcccatgccg
    13261 ttgatttggt caatcgcgag tcgctcggct tgaatggagc cggtttcgct gatggccatg
    13321 gtgcccgtgg cgctgtgcaa ctgtccgacc gtaacttcgg tgggtgttac ggcgatcgcg
    13381 gccttggccg agtctagtcc tggtttttcg gtgccggcgg agggcgtgct gcccttggct
    13441 ttttggcagc cgcctagggc ggcgccgagc gcgagtagaa gcgcgaagaa acggtagttg
    13501 agagaagaaa ttagagctgt cttgctcatc atgcactcct gtgcttgagg ggcgacgcaa
    13561 gcgcgtgagg aaacactcgg gttggggagc ccgcgtgttc ggggacctgt gttacggatt
    13621 caggtttcac ccgtgtatta ggagtgagcg tgtttcgttg gtgtgtgtgg tcggtgttgt
    13681 gacagtaaat ctacccatgt gagaggaaat cgaagtagag gtgggacgcg gctaggttcg
    13741 acacggctag gttcgacacg gcctgagacc acgtgtgcgc accgcgcggt tcgggtggcg
    13801 gcctcgaacg ggtcggcggc gtatcggcga cttatcgcgc gagtgtcact ggccgtctcg
    13861 cagcggcgac ggttcttgca gtgagccgca ttcgaaacat gacccaccga gccgactcac
    13921 ctcgccgcaa actcgcaacg cgccactctg catcacggac ctggccggcc tttctgagcc
    13981 tcgcggcagt gtgggcctgc gggcgcagcg acttgaagga cagcctttac gatgggccct
    14041 ggacaatcga ggagggcggg accggcggat tgtcgcagat cgctggcgga accccatccg
    14101 ggggaacggt ttccgcaggc ggcgcgggcg cgacgtcctt cggtggggcg ggcaacgcac
    14161 cttggggcgg taccgtcggc gacggcggaa gctccggaca gaccacgaat gcgagcggag
    14221 caggttccgg ctttggcggc atcggcggag aaggtggagc cgcggtaggt ggctttgccg
    14281 aaaccggcgg ctttgcccct gccggaggaa ctgtcgcatt cggcggcagc acggcattcg
    14341 gcggcagcac ggcattcggc ggcacgatcg gcgtcggcgg cgttacgctc tttggcggca
    14401 atactgcccg tggcggaaca acctccatgg gtggcacgac cgctgccggt ggagtgcccg
    14461 tctcgggtgg caccagcacg gcgggcggcg tcaccgctgc gggtggcaca tctggcatcg
    14521 gcggggcggg cagcggcgga cgcaccgaat tgggcggcgc cagctcaacg ggtggagtca
    14581 acctgggcgg agccaccagc ggtggtcgag gcggcacgac ttcgatgggc ggcacgactt
    14641 cgatgggcgg cacgaccgcc acgggtggtg cgggccgagg cggagccacg gcgagtggcg
    14701 gtacgagcgc gagcggcggc ctcactggca ctggcggaac cacccaacaa ccgcgctgcg
    14761 gtgacggccg cgttcaagcg cccgaagcgt gtgacctggg tgtgaagaac gccgatcgac
    14821 ccgccctcga actccgccag ggcgcgctca tccgggcggt attgcccatt gcccgaccgg
    14881 ccaccgccag tgccttctat gcctactcgt cgggcagcag tcacactggc ttcgaggcat
    14941 tgaacaccag ctcgttcttt tggtatcggg acagcgctac cggcgcgctc tccctgatac
    15001 agcatcatgg cgtcgacctt gactcaactg gggtacgcca accctcttcg gccctcagcg
    15061 tcgacatcac tggactgccc actaaaaccc tcgtcgtcat ctccgacgat gcgggtgaac
    15121 tcgtcaaaac cggccccaac agcgccttgg gcagcttcaa gttcaaccgc aactctgacg
    15181 ggggcatcct ctccaacttc gatttcccct ccgccttcag cgtcgaagtc aacgtcagcg
    15241 tcgacactgg caccacgctc ctccgcttca ttgacgacag tggcgttgcg atccccttga
    15301 acctcgacct ccccttcgac ctcatcgcgt acgccagccc cagcacctgc cgcaccacct
    15361 gcaccatccc tcgttgtggc gacggcatcc tcgacggcgg cgaaacctgc gacgacggca
    15421 acaccgtcac gggcgacggc tgctccgccg actgccgcac cctccgttaa ccctcgcccc
    15481 gagcttcccc tcaaaagggt gcgcccaatc aagagggtgc gcccaatcaa gagggtgcgc
    15541 ccaatcaaga gggtgcgccc aatcaagagg gtgcgcccaa tcaagagggt gcgcccaatc
    15601 a
//
LOCUS       DASTKU010001033         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_425197, whole genome shotgun sequence.
ACCESSION   DASTKU010001033 DASTKU010000000
VERSION     DASTKU010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425197"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFQ61_36670"
     CDS             complement(<1..512)
                     /locus_tag="VFQ61_36670"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET9960102.1"
                     /translation="MQIQSRNTTGHGESTGNGNSEDHSRNHSKNLVALLESRVEEWGE
                     RPFFRFLVEGELDGIQEELSFAALAGAARAIAARLTSEAARERPVLILVPPGPHFLKA
                     FFGCLYAGAIAVPMYPPDPARLQRSIERLQAIVNHCRSGLLLTTKEWSPMLAGAVHAS
                     GLRMVTLDAV"
     gene            575..1420
                     /locus_tag="VFQ61_36675"
     CDS             575..1420
                     /locus_tag="VFQ61_36675"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET9960103.1"
                     /translation="MTTPNSVPGYRQVPWPPIRDLVVDFVAAGRAVPSVYGFIEIDVT
                     DVKLKLATLAQRDRRISFTAYLTFCLGRALEKHPMIHGLRQGKHIVCFDDVDVNTLLE
                     KQKPDGSLIPVMYIVRSANRKSLTDINRELRAAVQRDLLDDPGVKRRARLLSFPAWVR
                     RGLLAWARRNPFSMKRHWGTVGLSNLGSAVSSRFAVGTSFTIVTLSVIAGSIYTKVMW
                     IDEKATPRQVLGVTLLFDHNLLDGGPAARFTEEFARTLELATGLDELLGDEPASETDA
                     TGAPP"
     gene            1510..1755
                     /locus_tag="VFQ61_36680"
     CDS             1510..1755
                     /locus_tag="VFQ61_36680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960104.1"
                     /translation="MHSYCPSHLTLQYGFVTLPKLAPNGTLASNHDSHAIAVGDPFLP
                     QLQGVRRPTPAPSANIDQWAAIGGLGVSFSVEIHNEP"
     gene            2162..>2428
                     /locus_tag="VFQ61_36685"
     CDS             2162..>2428
                     /locus_tag="VFQ61_36685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960105.1"
                     /translation="MYRASEPPDLGPTTLLTNEFVHVRSLPGEQIIEVWRSERPARTL
                     AEVDESWGKVERVLSCVARAHRSLLLDMRQARGRNDPAFESAVAE"
BASE COUNT          455 a          757 c          734 g          482 t
ORIGIN      
        1 tcgaccgcgt ccagcgtaac catgcggagc cctgacgcat gcacggcgcc cgcgagcatg
       61 gggctccact ccttcgtggt cagcaatagg ccacttcgac agtggttcac gatggcctgc
      121 agccgttcga tgctgcgctg caagcgcgcc gggtcgggcg ggtacattgg aactgcgatc
      181 gcacccgcgt acaaacagcc gaaaaacgcc ttcagaaagt gcggtccggg cggtaccaga
      241 atcaggaccg gccgttcgcg cgccgcctca ctcgtgagtc gcgccgcgat cgcgcgtgct
      301 gcaccggcca aggccgcaaa agacaactct tcttgaatac cgtcgagctc accttcgaca
      361 aggaagcgaa agaacggtcg ctctccccac tcctcgactc gggattcgag caacgcaact
      421 aggttcttcg aatggtttct cgaatggtct tccgaattac cgttaccggt tgattcgccg
      481 tggcccgtcg tgtttcttga ttggatttgc acgcaagact ttcgtgtgcc gcgctatccg
      541 cgctaaaaag ggtcgctccc aacccggaga acccatgacc actcctaaca gtgtgcccgg
      601 ctatcgacaa gtgccctggc ctccaattcg cgacctcgtc gtcgacttcg tggctgcagg
      661 acgagcggtt ccgtcggtgt atggcttcat cgaaatcgac gtgaccgatg tcaaactgaa
      721 gttggctaca ctcgcgcagc gagaccgacg catctctttt acggcatacc tgaccttctg
      781 tctggggcgc gccctcgaga aacatccgat gattcatggg ctacgccaag ggaagcacat
      841 cgtgtgcttc gatgacgtgg acgtgaatac attgttggag aaacagaaac ccgacggcag
      901 tctaatcccg gtgatgtata tcgtccgcag cgcgaataga aaatctttaa cggacatcaa
      961 ccgcgagttg cgcgccgcag ttcaacgcga tttgctggac gatcctgggg tgaagcggcg
     1021 agcacgcctg ctgagtttcc cggcttgggt acggcggggt ttgctggcct gggcccggcg
     1081 caacccattc tcgatgaagc gtcattgggg tacggtgggc ctatccaacc tcggctcggc
     1141 agtatcctcg cgattcgccg tcggaacctc gttcactatc gtgacgttga gtgtgatcgc
     1201 gggcagtatc tataccaagg tgatgtggat cgatgaaaag gccactcctc gccaggtttt
     1261 gggcgtgact ttgctgttcg atcacaacct cctcgatggc ggaccggccg ctcgcttcac
     1321 ggaggagttc gcgcgtactc tggagttggc tacaggtctg gatgagttgc tcggagacga
     1381 gcccgcgtcc gaaacggacg cgaccggcgc tccaccctga agggtcagaa tattggttgt
     1441 gccgctctct attccacctc gtcaacccga tctcgtgtgg cctatgcgga cactccgaat
     1501 ccgcaagaca tgcacagcta ctgcccctcg catctcacgc tccagtacgg gttcgtgacc
     1561 cttccgaagc tcgcgccaaa cggcacgctc gcgtctaatc acgactcgca cgccatcgcc
     1621 gtcggcgatc cgtttctccc gcagctgcag ggtgtgcgcc ggccgacgcc agcgccgagc
     1681 gcgaacatcg accaatgggc cgcgatcggc ggcctcggcg tcagcttctc ggtcgaaatc
     1741 cacaacgaac cctagctcct cggcagccgc catttacaac gagccgtcac cagcgattgc
     1801 gccaggctcg cggcgcgcgg gaactagtcg gcttcatcaa gctcaggtgt ttatgggaga
     1861 ccgcgcatac gccgagcgct cgtttccatc ccgtatcgtg acggtagcgc gtggcgcaac
     1921 ccgtcctgcg gttcagcttg gtggtttcga caggaagtct ggtacacggg cgcgcgctgc
     1981 caggtaccaa ggaggccggg cgatgcggcc ccttcccagg gaccagctcc aattgggatg
     2041 ggcttcgagg caatgtctaa cccgagggtg gaggcgagcc cgcagcgccc ttgctgtttc
     2101 gtgcggcgcc gctcgtctgt gctcgccacc tggccactct ggtcgtagct ttcggcaggc
     2161 gatgtatcgg gcgagcgaac caccagatct tggacccacg acgctgctga cgaacgagtt
     2221 cgtgcacgtg cgctcgttgc ccggcgagca aatcatcgag gtgtggcgca gtgaacggcc
     2281 ggcgagaacg ctcgccgagg tcgacgagtc atggggcaag gtcgaacgag tgctgtcctg
     2341 cgtagcccgt gcgcatcgga gtctgctgtt ggatatgcga caggcccggg gtcgcaacga
     2401 tccggctttc gagagcgcag tcgccgag
//
LOCUS       DASTKU010001034         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_204530, whole genome shotgun sequence.
ACCESSION   DASTKU010001034 DASTKU010000000
VERSION     DASTKU010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204530"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFQ61_36690"
     CDS             <1..229
                     /locus_tag="VFQ61_36690"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9960106.1"
                     /translation="RLSKWALNGFTIQLAHELRGQIAVNAFDPGWVKTDLGGSRAPGT
                     PDESARGALALVLEPFTSTGKFWKDGQQIPF"
     gene            254..1009
                     /locus_tag="VFQ61_36695"
     CDS             254..1009
                     /locus_tag="VFQ61_36695"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HET9960107.1"
                     /translation="MTKLSVNVNKIATLRNTRTLEIPSLTHLSTIALRAGAHGITVHP
                     RPDQRHIRPDDVPAIAAVVASFAGAEYNIEGNPFHGLVEHCESVKPTQATLVPDAREA
                     FTSNHGWNLVELFAPERKALAHAIERLHVIGCRVSLFMDPVADMMPIARELGADRVEL
                     YTEPYAAAHASAKSQAQASAVVERYAEAARAAIDQGLQVNAGHDLSLANLPDFLRAIP
                     SIAEVSIGHALIADALEFGLADTVRRYLRACGN"
     gene            complement(1023..1370)
                     /locus_tag="VFQ61_36700"
     CDS             complement(1023..1370)
                     /locus_tag="VFQ61_36700"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET9960108.1"
                     /translation="MGHFPAFIKNPLNRIASSSQYTQGIEGYLFDGLDGSQAALWECH
                     ETRTSQPHVHAFDEYMLVIEGHCRVTLPSGEVELGPGQELHIPAGTPQSVLVLAGTRT
                     LHVFGGKRAERAG"
     gene            complement(1418..>2741)
                     /locus_tag="VFQ61_36705"
     CDS             complement(1418..>2741)
                     /locus_tag="VFQ61_36705"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HET9960109.1"
                     /translation="SPLIALMKDQVDALTEFGFRATYLNSSLTPEERRDRVARLRRNE
                     FEIVYAAPEGLEASAGRTIFDLNLGLIAVDEAHCISQWGHDFRPAYRNLQGLKRRFPK
                     TPVLALTATATPEVVRDIIDQLGMREPNVFRGSFFRKNLRITALPKSTLPKSTRHSIC
                     ELVRARKGESGIIYCLSRKSCEATAEHLLGQGLNAAHYHAGMDPAERSRVQDAFRNGK
                     IDVVVATIAFGMGIDKSDVRFVLHHDLPRSIEGYYQEIGRAGRDGLPSDCVLFYSFAD
                     VKAYERMAEQQEDQDAAARLVNQARETFRLVEAGGCRHQNIVGYFHEQLDPCGTACDR
                     CTNLKARSIAERRPTKASRSTEPRPTETLSSTEIGVDVDLLARLKQERRRIAEELRVP
                     AYVVFTDATLLEMAQRRPQSEAELLSVNGVGLRKLDRYGSQFLSILRG"
BASE COUNT          497 a          908 c          827 g          509 t
ORIGIN      
        1 tcgattgagc aagtgggccc tcaatggctt caccatccaa ctcgcccatg agcttcgcgg
       61 ccagatcgcg gtgaacgcct tcgatccggg ctgggtgaag acggatctgg gtggcagccg
      121 cgcgccgggc accccggacg agtccgctcg cggcgcgttg gcgctggtct tggagccgtt
      181 cacgagcaca ggcaagttct ggaaagacgg ccagcagatt ccgttttgaa tcgagggagc
      241 gaggggttca ccgatgacca agctgtccgt caacgtcaac aagattgcca ctctgcgcaa
      301 cacccgcacg ctcgagatcc cgagtctcac ccacctctct accattgcgc tgcgcgcggg
      361 tgcgcacggc atcaccgttc acccgcgccc ggatcaacgc catatccggc cggacgacgt
      421 gcccgcgatc gcggccgtgg tcgcgagttt tgcgggtgcc gaatacaaca tcgagggaaa
      481 ccccttccac ggcttggtcg aacactgcga gagcgtgaag ccaactcaag cgacactggt
      541 gcccgacgct cgcgaggcct tcaccagcaa tcacggttgg aacctcgtgg agctgttcgc
      601 tccagagcgc aaagccctcg cccatgccat cgaacggctc cacgtcatcg gttgccgcgt
      661 gagtctgttc atggatccag tggcggatat gatgccgatc gcgagagagc tcggcgccga
      721 tcgcgtggag ctctacactg agccttacgc cgcggctcac gcctccgcca agagccaagc
      781 ccaggcctcc gcggtggtcg agcgctacgc cgaagccgca cgcgccgcga tcgatcaggg
      841 cctacaggtc aacgcggggc acgacttgag cctcgcgaat ttaccggact tcctgcgcgc
      901 catcccgagt atcgcggagg tgtcgattgg tcacgccttg attgccgatg cgctcgaatt
      961 cggcttggcc gatacggtcc gacgctacct gcgcgcttgc ggaaactgag cgggcgctgc
     1021 gtttatcccg ctcgctccgc ccgctttccc ccaaacacgt ggagcgtccg ggtgcccgcg
     1081 agcacgagca cgctttgcgg cgtccccgcc gggatatgaa gctcctggcc gggccccaac
     1141 tccacttcgc cgctcgggag agtcactcgg cagtgccctt cgatcacgag catgtattcg
     1201 tcgaacgcat ggacgtgcgg ctgtgaagtg cgcgtctcgt ggcactccca gagcgcggct
     1261 tggctgccgt ccaggccgtc gaacaggtag ccctcgatac cctgggtgta ttgggaggac
     1321 gaagcgatgc ggttcagcgg gttcttgatg aaagcaggaa agtgccccat ccccccgtgc
     1381 tgagccagtt actgacgcgc ttcaatccta tgtggactca gccccgcagg atgctcagga
     1441 attgtgaccc gtagcggtcc aacttgcgca agcctacgcc gttcacggac agtagctcgg
     1501 cctcgctctg aggccggcgt tgggccatct cgagcaacgt cgcgtccgtg aaaaccacgt
     1561 acgcgggcac gcgcagctct tccgcgatcc tacgccgctc ttgcttcagc cgagcgagca
     1621 agtccacgtc cacgccgatc tcggtggaac tcagcgtctc cgtgggccgc ggctcggtgc
     1681 tgcgggacgc tttcgtcggc cgccgctcgg cgatacttcg cgctttcaag ttggtgcagc
     1741 gatcgcaagc cgtgccgcag ggatcgagct gctcgtgaaa atagccgact atgttttggt
     1801 gacgacagcc gcccgcctcg accagccgga acgtctcccg cgcctgattc acgagccgcg
     1861 ctgccgcgtc ctgatcctcc tgctgttccg ccattcgctc gtacgccttc acgtcggcga
     1921 acgagtaaaa gagcacgcaa tcgctcggca atccatcgcg ccccgctcgc ccaatctcct
     1981 ggtaatagcc ttcgattgaa cgcggtaagt cgtggtgcag cacgaagcgc acatccgatt
     2041 tgtcgatgcc catgccgaac gcgatggtcg ccaccaccac gtcgatcttg ccgttacgaa
     2101 aggcatcttg aacccgcgag cgctccgcgg gatccatccc ggcgtggtaa tgggctgcgt
     2161 tcaagccctg gccgagcagg tgctccgccg tcgcttcgca gctcttacgc gacaaacaat
     2221 agatgatgcc gctctcgcct ttgcgtgccc gcaccagctc acaaatcgag tggcgcgtgg
     2281 atttgggcaa cgtgctcttg ggcagcgcgg tgatgcgcag gttcttacgg aagaacgagc
     2341 ctcggaacac gttgggctcg cgcatcccga gttgatcgat gatgtcccgc accacctcgg
     2401 gcgtggcggt ggcggtgagc gccagcaccg gcgtcttggg aaagcgccgc ttcaagccct
     2461 gcaggttgcg gtatgccgga cgaaaatcgt gcccccattg gctgatgcaa tgggcctcat
     2521 ctacggcaat caaacccaaa ttcaaatcga agatggtgcg gcccgcggag gcttccagac
     2581 cttcgggtgc ggcgtacaca atctcgaatt cgttccggcg caggcgagcc acgcgatccc
     2641 gtcgctcctc cggtgtcagc gacgaattca agtaagtcgc tcgaaaaccg aactcggtga
     2701 gcgcgtccac ttgatctttc atcaacgcga tcaggggcga a
//
LOCUS       DASTKU010001035         3155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_426053, whole genome shotgun sequence.
ACCESSION   DASTKU010001035 DASTKU010000000
VERSION     DASTKU010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426053"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1242
                     /locus_tag="VFQ61_36710"
     CDS             151..1242
                     /locus_tag="VFQ61_36710"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HET9960110.1"
                     /translation="MTAKAANKLSIRRAPHGSPIAPQVSSRMARRLILLAESLGLSAE
                     KLCQAHDLNPELLEDRTARIPLSNVLSGLEQILEETRHPALGLALARCAAPDSYHTPA
                     LLLMASACLRDGFQHAFQYQRLWGDGDRFTFSTGIEVGRPARESIIAFRVPGARRAGH
                     SILEVCALAETMLAVRYLTGELGARACYLALPSTLDSPTELAKHFGITPELGSAKACV
                     GFTEDLVTAPLPHANALFLQVFERQAREELAALPPLDDWLARVRAAISQGFARGRYAL
                     VDSARSVGMSERTLERRLAERGLTYQGLVDAIRKPQALHLLRAGHPVDEIAVLLGYSE
                     RSSFHRACMRWFRMTPAALRRAHTTGTDR"
     gene            1321..2418
                     /locus_tag="VFQ61_36715"
     CDS             1321..2418
                     /locus_tag="VFQ61_36715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HET9960111.1"
                     /translation="MASLFDELTLGSVRLRNRIAVSPMCQYSATDGVVNDWHLMHLGS
                     LAAGGAALVVAEATAVSPEGRITPGDTGLWNDHQAEAWQPIVRFIERQGAVPGIQLAH
                     AGRKASANRPWEGDDHLAPDHPEAWPTIAPSALAFGANLPRVPREMTLDEIARVRTDF
                     VAAARRAHAAGFKWLVLHFAHGYLAQSFFSPLANQRTDAYGGSFENRARFLLETLAAV
                     REVWPAELPLTARIGVCDYVPGEQPMEESIELIRRMKATGLALADVSLGFNTPDASRV
                     PWGEHAFLAPIAARIRREAELPTAMSWNINQPAKANELVVQGSLDLIMLAKALLSDPH
                     WPYHAAQALGREAPQNTLPPQYAHWLRGYRG"
     gene            2431..>3155
                     /locus_tag="VFQ61_36720"
     CDS             2431..>3155
                     /locus_tag="VFQ61_36720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960112.1"
                     /translation="MRGKAARLLFNSIQFLVFFPIVTLLYFVLPGKWRPMMLLAASSW
                     FYMAFIPAYILILAGTILVDYGAGLGLERLRNDPRRRLFLIASLAANIGVLAIFKYYN
                     FFSTNIDHAAAALGLQFALPALKIALPVGLSFHTFQAMSYTLEVYRGHQKAERSLLIF
                     ALYVMFYPQLVAGPIERPQNILHQLHEVHAFDGRKVSEGLELMLWGMFKKVVVADRLA
                     IAVNAVYSAPRAHQGQELLLATYA"
BASE COUNT          578 a         1042 c          930 g          605 t
ORIGIN      
        1 ccaaaagcaa gcgacgaact cgacgaggcc catgcgagac gctcagatta cgattccgag
       61 cggcgcggaa cctatggcgc gtaacgccag aaggattgac ccaagccgcc attgcacccg
      121 gctcggagct tcgttaagca aaccgtgccc atgacggcga aggctgccaa caagttatcg
      181 atccgcagag ccccgcacgg ttccccgatc gcgccccagg tctcttcgcg gatggcgcga
      241 cgcttgatac ttctggcgga aagcttggga ttgagcgcgg aaaagctctg ccaagcgcat
      301 gacttgaacc ccgaactgct cgaagaccga accgcccgta ttccgttgtc gaatgtgctg
      361 tcagggctcg agcagatcct cgaagagacg cgccatcccg cgctcgggct cgcgctcgcc
      421 cgctgtgccg cccccgacag ctaccacacc ccagcgctgc tcctgatggc cagcgcttgt
      481 ctgcgggatg gctttcagca cgcctttcag tatcaaaggc tctggggaga cggtgatcgc
      541 ttcactttct cgacgggcat agaggtggga cgccccgcac gcgagagcat catcgctttt
      601 cgagttcccg gcgctcgtcg cgcggggcac tccatcctcg aagtgtgcgc actggcagag
      661 acgatgctcg cggtccgcta cctaaccggt gaactcggcg cgcgtgcatg ctatttggcc
      721 ttaccgagca cgctcgattc gccgaccgag ctcgccaaac atttcggcat cactccggaa
      781 ctcgggtccg ccaaggcctg cgtcgggttc acggaagatc tagtgacggc tccgctaccc
      841 cacgccaacg cgctctttct acaagtcttc gagcggcaag ctcgagaaga gctcgccgct
      901 ttgccacctc tcgacgattg gctcgcgcgg gtgagagccg caatttccca gggcttcgcg
      961 cgtgggcgct acgcactcgt agatagtgcg cgcagcgtag gcatgagcga acgcaccttg
     1021 gagcgccgct tggcggagcg cgggctcacg tatcaaggcc tcgtggacgc aatcagaaaa
     1081 ccccaagccc tacatctgct ccgcgcaggt cacccggtgg atgaaatcgc ggtgctgctg
     1141 ggctactcgg agcgctcttc gtttcatcgc gcgtgcatgc gctggtttcg aatgacgccc
     1201 gcggcgctca ggcgcgctca cacgaccggc acggatcgct gaccccaggt ctagcctcga
     1261 gcgagcaacc gtggagtttg ggcttgcgag cgcacgcgcg ctccggcaaa aaggctctcc
     1321 atggcgtccc tattcgatga actcacgctc ggatcagtac ggttgcgcaa ccggatcgcc
     1381 gtatccccca tgtgccagta ctctgcaacg gatggcgtgg tgaacgactg gcacctgatg
     1441 catctcggga gcttggccgc agggggcgcc gcgctcgtgg ttgccgaagc caccgcggtg
     1501 tctcccgagg gcagaattac ccctggtgat accggccttt ggaatgatca ccaggccgag
     1561 gcgtggcaac ccatcgtacg cttcatcgag cgccaaggcg ccgtaccggg cattcagctc
     1621 gcgcacgcag gtcgtaaagc cagcgccaat cgaccgtggg aaggcgacga tcatctcgcg
     1681 ccggatcatc ccgaagcctg gcccacgatc gcaccctctg cactagcgtt tggcgccaac
     1741 ttgccccgcg tgccgcgcga aatgacgctc gacgagatcg ctcgcgttcg tactgatttc
     1801 gtcgctgctg cgcgccgtgc ccatgcggcc ggcttcaagt ggctggtgct gcactttgcg
     1861 cacggctatc tcgcacaaag cttcttttcg ccgctcgcca accagcgcac cgacgcatac
     1921 ggcggcagct tcgagaaccg agcccgcttc ctgctcgaga cgctagctgc cgttcgtgag
     1981 gtctggcccg ccgagctgcc gctgacggca cgcattggag tgtgcgacta cgttcccggc
     2041 gaacagccca tggaagaatc gatcgagcta attcgacgca tgaaggcgac cggcctcgcg
     2101 ctggcggacg tgagcctcgg cttcaacacg ccggacgcga gccgagttcc ctggggtgag
     2161 catgcttttt tggcgccaat cgcggcgcgc atccgccgcg aagccgagtt gcccacggcc
     2221 atgtcctgga acatcaacca acccgcgaaa gccaatgagc tcgttgtgca aggctcactc
     2281 gacctcatca tgttggcgaa agctctgctc tccgatccac attggccgta ccacgccgcg
     2341 caggccttgg gtcgcgaagc accgcagaac accctgcccc cgcagtacgc gcactggctc
     2401 cgcggatacc gcggctaatc cccgaatcgg ttgcgaggaa aggcggctcg actgctcttc
     2461 aactcaatcc agttcctggt cttcttcccg atagtcacgc tgctgtactt cgtgctcccc
     2521 gggaagtggc gccccatgat gctgctagcc gccagctcgt ggttctacat ggcctttatc
     2581 cctgcctata tattgatctt ggcggggacg atcctagtgg actacggggc cgggttggga
     2641 ctcgagcggc tgaggaacga cccacgccgc cgactgtttt tgattgcgag cctcgctgcc
     2701 aacatcggcg ttctcgcgat cttcaagtac tacaatttct tctcgaccaa tatcgatcac
     2761 gcagcggccg cactcgggct ccaattcgcg ctgcccgcgt tgaagatagc ccttccggtt
     2821 gggctctcgt ttcacacctt ccaggcgatg agttacacac tggaggttta tcgagggcat
     2881 caaaaggccg agcggagtct gctcatcttt gccttgtacg tgatgttcta tccgcagctg
     2941 gtggcgggcc ccatcgagcg acctcagaac atcctgcatc aattgcacga ggtccatgcg
     3001 ttcgatggcc gaaaggtcag cgagggcctc gagctcatgc tctgggggat gttcaagaag
     3061 gtcgtcgtcg cagatcgctt ggcgatcgcg gtcaatgccg tctattcagc gccccgagcc
     3121 caccaagggc aggagttact cctcgcgacg tacgc
//
LOCUS       DASTKU010001036         5768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1545497, whole genome shotgun sequence.
ACCESSION   DASTKU010001036 DASTKU010000000
VERSION     DASTKU010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545497"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1802)
                     /locus_tag="VFQ61_36725"
     CDS             complement(<1..1802)
                     /locus_tag="VFQ61_36725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960113.1"
                     /translation="MTLAGCSSEEPKAIFVGGPGALGERCKRSEDCASGFCVRLDDVG
                     GVCSLECGTDADCPHAENWGCLQAGSTPFHVCACRPIAAIEVCGDQLDNDCNGQVDNC
                     RLCAGKPVPENDPKNCGACGKACAREESCVQGTCACPAGEPDRCGEECVNLKEDEQHC
                     GSCKTACNPGQVCNAGSCECPDPVAPDYCAATGCTDTKRDIFNCGECGFSCPSGQICQ
                     DGACSCPFASLPDYCPRVGCTNFAADGGNCGACGESCTFARTCVAGKCTCPEETPDFC
                     DGLGCVDLKNDDRQCGSCGTACLSDQYCANGMCVCAVGQVSCNGVCTDTQRSNANCGA
                     CGAACPSGTTCLNGACTCFEGKATLCGDECALIDTNTQHCGKCGHACQAGEECFRGEC
                     LCPSGLFCDGRCVSTSDAANCGGCGKSCGPTQACWSGMCTCLGSSLTACGASCVDTQY
                     DLNNCGGCGQVCPLGMTCSDFRCGCPWGTSYCADAHACLDLKSDPEHCGACGKACPTQ
                     QFCSNGTCQCENWFETYCAGSEKCVNLAGSDEANCGACGRSCRSGTHCSSGSCVCDQD
                     GYLECNDQCVNLNEDLRNCGSCGNTCAEGALCLAG"
     gene            complement(2242..2314)
                     /locus_tag="VFQ61_36730"
     tRNA            complement(2242..2314)
                     /locus_tag="VFQ61_36730"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2279..2281),aa:Gly,seq:gcc)
     gene            complement(2444..2513)
                     /locus_tag="VFQ61_36735"
     tRNA            complement(2444..2513)
                     /locus_tag="VFQ61_36735"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            2642..4219
                     /locus_tag="VFQ61_36740"
     CDS             2642..4219
                     /locus_tag="VFQ61_36740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960114.1"
                     /translation="MHREWFTNLRYRVSSEQIRAATAFRSAGDFMGACAATRVWPSFD
                     LEAVGREYGAALERELELTLQGFAPDLLRWNIPRNEHSITAGLKLRLVEWQWETPLAL
                     WLETPRTATSPQDLFLHFGPSPAGLSNLERCHWDARHVAELLSHAGGRSRLPFFPPDG
                     QARLWTWHTDPSSIDPALEPEARFEKVTLLQDRGNSGFAEAASWAGFEVTPEVIALTK
                     SQYSNYERWDLWPVQEIDLIRLNRLVRELHSTAGVPSAYVRFGSWASRILWIHELDAA
                     RPLLDAVSVPDRASKAEFPPKGAREIAWHECSRSPDLERLRRGWLKPSELHPLVLRAF
                     FPAWPGELSEAGPAPVDDIEPLRVRCGEEWHWIGLRDGRVQALQHAPEQEAHEQVMAA
                     LGAPIPGCFRALQAWRSWGRSGRVPRRLRERLKELKTRVAHGDADGVFRFLEQDPALA
                     QGSAGIGSSGSSMLHYLPWLGVECLPRLLALGLDLEARDLEGQTPLQVALKEGRPEVA
                     AALLAVGAKPAPPETSP"
     gene            4216..5442
                     /locus_tag="VFQ61_36745"
     CDS             4216..5442
                     /locus_tag="VFQ61_36745"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9960115.1"
                     /translation="MTDSQNRSDVASSATERALNNSDALVAQLRETRLEPGRHVQLEA
                     LALAVCANLPVLLWGEPGIGKSAILSQLAEQYGLPLETVIASVHEPSDFAGLPIVGEN
                     PEQGVPMAPPDWAVRLAARGTGLLFFDELSTAPPAVQAALLRVVLERRVGSLNLPDRV
                     RIVAAANPPASAADGWHLSPPLANRFVHLQWAHDPPTVARGLAGTWPYVAIPVVDPSR
                     SVSAIARARGSVSAFLTARPGLAHSMPREGEARGRAWPSPRTWEMALRLLAVSFACPN
                     ASIEARSELMVGTLGDGAGLEFVSFLESLDLPDPDHVLARPEEFTLPQRGDRQLAFLT
                     AVVSAVQSRLSESRWRAAWVVLGKAVEAGIPDVAARAALDLAQLRDAKWLPPAQALAF
                     GEVLRLSGVNLGSARR"
     gene            5464..>5768
                     /locus_tag="VFQ61_36750"
     CDS             5464..>5768
                     /locus_tag="VFQ61_36750"
                     /inference="COORDINATES: protein motif:HMM:NF024601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960116.1"
                     /translation="MTGLDRTKLLAARFRAANERPYFASALYALFVVETRAVPTMAVD
                     RFFRCYVNPEFVAATRVDELAAVWIHEAGHLLRAHAERAERLPPELSKDHVRVNIAM"
BASE COUNT         1003 a         1863 c         1787 g         1115 t
ORIGIN      
        1 cccgcgagac acaacgcgcc ctccgcacac gtgtttccgc aagaaccgca gttcctgagg
       61 tcctcattga ggtttacgca ttggtcattg cattccaggt agccgtcctg gtcgcacaca
      121 caggaaccag acgagcagtg agtgccgctc cggcacgacc tcccgcacgc gccacaattg
      181 gcttcatcag agcccgcgag attcacgcac ttctcgcttc cggcacaata ggtctcgaac
      241 cagttctcac attggcatgt cccgttcgag cagaactgtt gagtcgggca ggctttgccg
      301 cacgcgccgc aatgctcggg atcagacttc aaatccagac aggcgtgagc atcggcgcaa
      361 tagctcgttc cccagggaca gccgcacctg aaatctgagc aggtcattcc aagtggacag
      421 acttgtccgc agccaccgca gttgttgaga tcgtactgcg tgtccacgca actggcgccg
      481 caggcagtca gcgacgagcc caggcacgtg cacatcccac tccagcaggc ctgagtcgga
      541 ccgcaggact tgccacaccc gccacagttg gcagcgtctg aggtcgatac gcagcggccg
      601 tcacagaaca agccgctggg gcatagacat tccccccgga aacactcttc tccggcctga
      661 caggcatgtc cacattttcc gcagtgctgc gtgttggtgt cgattagagc gcattcatcg
      721 ccgcacagcg tggcctttcc ttcgaagcag gtgcaggcac cgttcaagca tgtcgttcca
      781 ctcgggcagg ctgcaccgca agccccacag ttcgcgttac tcctttgagt gtccgtgcaa
      841 acaccattgc agctgacctg accgacggcg catacacaca tgccgttcgc acagtactgg
      901 tccgataggc acgctgtacc gcatgaaccg cactggcgat catcgttctt cagatcgacg
      961 caacccaaac cgtcgcaaaa atcaggcgtt tcctcggggc aagtgcattt cccggccacg
     1021 caagtgcggg caaaagtgca cgactcgccg caagctccac agttcccgcc atcggctgcg
     1081 aagttcgtgc aaccaacgcg cgggcaatag tcggggaggc tcgcaaacgg gcaagagcaa
     1141 gcaccgtctt ggcaaatttg tccgctcggg cagctgaagc cgcactcacc acagttgaag
     1201 atatcccgct tcgtgtccgt gcagcccgtg gctgcgcaat agtctggcgc gactggatcg
     1261 ggacattcgc agctgcctgc gttgcagacc tgcccaggat tgcaggctgt cttgcagctc
     1321 ccgcagtgct gctcgtcttc cttcaggttc acgcactcct cgccgcagcg atccggttcg
     1381 ccagcgggac aggcacaggt cccctgaaca caggactctt cgcgcgcaca cgctttcccg
     1441 cacgcaccgc agttcttcgg gtcgttctcc gggacgggct taccggcaca gagacggcag
     1501 ttatccacct gcccgttgca gtcattgtcc aattggtcac cgcagacctc gatggccgca
     1561 atcggacggc acgcgcagac atggaacggc gtcgagccgg cttgcaagca gccccaattc
     1621 tcggcgtgtg gacagtccgc gtccgttccg cattccagac tgcagactcc gcctacgtca
     1681 tccaagcgca cgcagaagcc cgaggcgcag tcctccgaac gcttacagcg ctcgcccagc
     1741 gcacccgggc cacctacgaa aattgctttg ggttcttccg aactgcaccc ggcgagcgtc
     1801 aaaacggcga atagcagtgc ccaacttcgc ggcgcgagtg ccgacgctcc actcgatagt
     1861 tctgccgcaa aatcgcagct cccgtcgtcc tttcacccgg gggctgttct cgtttctgtt
     1921 gctcccccgt tctggatcct ctcggcaacg ccggtccgtg ctcgctccgc tgcgcgcggg
     1981 ccgaggccct caaggccatg ctcgcgccgc tcccggcgct ccgcgtggcc gagggtccta
     2041 accaacatcc agtctactct cgcccgaaat tgggactagg cggcgcgaag tccacgctta
     2101 gcaagctggc gcgtcggccc ccgcgggccg ccctcgccat cgagtttcgg actggagcgg
     2161 cgttccaagc cagactcttc aacgacgctt agactctctt gagacaggcg gccgtgttga
     2221 tgcggcggcc gtgaaactgg gcgagggtga ccggattcga accggcgtct tccagcctgg
     2281 cgagctggcg cgaggacctc tgcgctacac cctcacgttc ggacgatgcc attgctcgag
     2341 tgtgcgcggc tactccgttt tgatggcggc cacgcttttt cggatttgct tgatccccgg
     2401 cttgcctgcg agaaggcgac gcgcgcggcc aagccaagac ggttgagggc gatcggattc
     2461 ggaccgatgt cttccgcttg gattcggcgc gacgacctct gcgctacgcc ctccgggctg
     2521 gctctattcc acggctgcct caagagggaa agggatccgc gcgacaggga ggtttgacac
     2581 cgtcaattgg caacgggggt tgcggaccct cgtgcagctt tggcatggta agagacgccg
     2641 catgcaccgg gagtggttca cgaatctgcg ttatcgggtg tcgagcgagc agatccgtgc
     2701 ggcgacggcg tttcgttcgg ccggggactt catgggcgcc tgcgccgcca ctcgggtttg
     2761 gccctcgttc gacctcgagg ccgtgggtcg agagtacggg gcagcgctcg agcgggagct
     2821 cgaactgact ctccagggct tcgcacccga tctcttgcgc tggaacatac cgcgcaacga
     2881 acactcgata acagcgggtc tgaagcttcg gctcgtggag tggcaatggg agacgccact
     2941 ggcgctctgg ctcgagaccc cgcgcacagc cacgagccct caagatttgt tcttgcactt
     3001 tggaccaagc cccgccggcc tgagcaacct cgaacgctgt cactgggatg ctcgccacgt
     3061 agcggagctt ttgagccatg cgggcggtcg cagccgtctg ccgttcttcc cgcctgacgg
     3121 ccaagcgcgg ctatggacgt ggcacactga tcccagctcg attgatcccg ctctcgaacc
     3181 cgaagcgcgg ttcgaaaagg tgaccctgct ccaggatcga ggaaactctg gctttgcgga
     3241 agcggcttca tgggcaggat tcgaagtcac tcccgaagtg attgcgttga ctaagtcgca
     3301 gtattccaat tacgagcgtt gggatctctg gcccgtacaa gagatcgatt tgattcgctt
     3361 gaatcgtctc gttcgggaat tgcattcgac cgctggagtt ccgagcgctt acgtgcgatt
     3421 cggtagttgg gcatctcgga ttttgtggat ccacgaactc gacgcagcca ggccactctt
     3481 ggacgcggtc tccgtgcccg atcgcgcctc caaggcggag tttccgccga aaggtgcgcg
     3541 cgaaatcgct tggcacgagt gttcgcgctc tcccgacttg gagcggttgc ggcgcggttg
     3601 gctgaagccg agcgaactgc acccgctcgt gctgcgcgcg ttctttccgg catggcctgg
     3661 agagctctca gaagctggcc cggctcccgt ggatgatatc gagccgctgc gcgtgcgctg
     3721 cggcgaagag tggcactgga ttgggctgcg agatgggcga gtccaggcgc tccagcatgc
     3781 cccggaacaa gaagcccatg agcaggtgat ggccgcgctc ggcgcaccga tccccggctg
     3841 ctttcgggcg ttacaagcct ggagaagttg gggacgctct ggacgcgtgc caagacggct
     3901 tcgagaacgc ctgaaggagc tcaagactcg tgtggcccac ggtgacgccg acggggtgtt
     3961 caggtttctg gagcaagatc cggccttggc tcaaggtagc gccggcatcg gcagttccgg
     4021 ttcgagcatg ctgcactatc taccttggct tggggtggaa tgcttgccgc gcttgttggc
     4081 cctaggcttg gatttggagg ctcgcgatct cgaaggtcag acccctttgc aggttgcgct
     4141 caaagagggt cgccccgaag tggcggccgc actgctcgct gtgggcgcga agccagcgcc
     4201 cccggagacc tcgccatgac cgactcgcag aatcgttcgg acgttgcttc gagtgcgacg
     4261 gagcgcgcgc tgaacaactc cgacgcacta gtcgctcagc tgagggagac ccgactcgaa
     4321 cccggacgtc acgtgcagct cgaggcgctc gcgctcgcag tgtgcgccaa tcttccggtg
     4381 ctactttggg gtgagccggg catcggcaag tcggccatcc tgagccaact cgctgagcag
     4441 tacgggctcc ccctcgagac cgtgatcgcc agcgttcacg agccctccga ctttgcagga
     4501 ttgccgatcg tgggcgaaaa cccggagcaa ggcgtgccca tggcgccgcc cgattgggcc
     4561 gtgagactcg cggcgcgtgg caccggactg ctcttcttcg acgagctatc tacggcgccc
     4621 ccggcggtgc aagcggcgct cttgcgcgta gtgttggagc ggcgcgtggg cagcttgaat
     4681 ctgcctgacc gcgtacgcat cgtggccgca gcgaatccgc ccgccagcgc ggccgatggc
     4741 tggcatctga gcccgccgct cgccaatcgc ttcgttcacc tgcagtgggc gcatgacccc
     4801 cctacggtcg cacgagggct cgccggcacc tggccctatg ttgccattcc cgtcgtggac
     4861 ccatcgcgaa gcgttagcgc catcgctcga gctcgtggct ccgtttccgc attcttgacc
     4921 gcgcggcctg gcttggctca tagcatgccc cgagaagggg aagcccgcgg tcgagcttgg
     4981 ccttctccga gaacgtggga gatggctctg cgcttgctcg cggtcagctt cgcctgtcca
     5041 aatgccagca tcgaagcgcg ctcggagttg atggtcggta ccttgggaga cggagcaggg
     5101 ctcgagttcg tttcattctt ggagtcgctc gacttgcccg accccgacca tgtactcgct
     5161 cgacccgagg agttcacgct gccccagcgc ggagatcgtc aattggcttt cctcacggcg
     5221 gtagtatcag ccgtgcagag tcgactgagc gaatcgcggt ggcgggcggc gtgggtggtt
     5281 ttgggcaagg cggtcgaggc gggaattccg gatgttgccg cacgagccgc cctcgacttg
     5341 gcgcaactgc gcgacgccaa gtggctgcct ccagcgcaag cgctggcctt tggcgaggtg
     5401 ttgcggctgt ccggagtcaa cttgggatca gcgcgacgct agtttggcag gcgtggaggc
     5461 taaatgactg gtctcgatcg cacgaagctc cttgcggcac gctttcgagc ggccaatgag
     5521 cgaccctatt ttgcctcggc gctctacgcg ctattcgtgg tggagacccg cgctgttccc
     5581 accatggccg tcgatcgctt cttccggtgt tacgtcaacc cggagttcgt ggcggccact
     5641 cgggttgacg aattggcggc ggtttggatt cacgaagcgg gtcacctgct acgggctcac
     5701 gccgagcgcg ccgagcgcct accgcccgag ctgagcaaag atcacgtgcg cgtcaacatc
     5761 gccatgga
//
LOCUS       DASTKU010001037        17666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_205054, whole genome shotgun sequence.
ACCESSION   DASTKU010001037 DASTKU010000000
VERSION     DASTKU010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 17666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17666
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205054"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFQ61_36755"
     CDS             <1..620
                     /locus_tag="VFQ61_36755"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9960117.1"
                     /translation="ESATLAERNAYPDLTVGAGIMYRGALPPMWLVTLGAPVPIFSGA
                     RQSHAAAESRAWASAAQSQVAELEQLLRLRAQERELAFGATLDMLAVYDQGLLVQSQA
                     TAESALTQYKVGKGSFSAVLEAQAGLVADQEGYLALLANAHRILIAEAEVSLADTAIP
                     SGGAASGGMPGASAKPMNAPAMSSTAGASTSPAAAPSTAGSSSGM"
     gene            626..2335
                     /locus_tag="VFQ61_36760"
     CDS             626..2335
                     /locus_tag="VFQ61_36760"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET9960118.1"
                     /translation="MTSREPPPPESPLLTEPPLGDTPAKAEARPRTGYSGWALLIVGA
                     LSGVVGSAATFLALQQLGSGSPFKHEHASEAPLYQCPMHPTIIQDHPGNCPICGMKLV
                     KVEKSKVQNSVASAPGNASATHGAAGSGPGTASSTPTPSVPGLASVEIDPERQQLIGL
                     RTAEVTRGPVGGAWRTVGRVAIDETRVRHVNLKIPGFVERIYVDYIGKKVGKGDPLFS
                     IYSPELLSAQEEYLLARKTQTDLSKMGGPLAPSDDGMVAAARRKLELWDISAAEMDNL
                     VRTGQPKKSMTLYSPASGVVTKKDVVEGMKLDAGAMPYEIVDLSSVWVLADVYESELR
                     FIKQGMSANLTLAAFPNREFKGKVVFLDPLLDPQTRTVKVRLTFPNPTGELRPEMFGD
                     VVLFGAAHEGLRVPPDAIIDSGIEKVVFVAVGAGKFEPRRVQVGNGDETFVEVVSGLE
                     PGERVVVRANFLIDSESRLRASLSAVGREENPIPPAESARVLPGSVNPNAVNPNAAAA
                     NTAAAASGAGTPKANAAPGAVAPAKPPQNAPGKAQTVAPSPNKAAQDHSAHTGHDHSG
                     HMP"
     gene            2332..5604
                     /locus_tag="VFQ61_36765"
     CDS             2332..5604
                     /locus_tag="VFQ61_36765"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HET9960119.1"
                     /translation="MIKAIIRFSAENRYLVIAATIVALVFSWWSMRQIPLDALPDLSD
                     TQVIVYGRWDRSPDIIEDQVTYPITTALLGAPKVKAIRGFSDFGFSYVYVIFEDGTDM
                     YWARTRVLEYLSKITSQLPAGVKVELGPDASSVGWVFQYALLDKTGKHSSDELRSYQD
                     WFLRYAVQSVPGVSEVATVGGQVRQYQVTVNPNSLAAYKLPLESVIDAVRKGNNDVGG
                     RLVELSGREYMVRGRGYVKNIHDIEDIVLRSGAGTPVRIKDVASVALGPEMRRGIADY
                     NGQGDAVGGIVIMRQGENALNVIERVKTKLDELKPSLPPGVEVVTTYDRSELIERAID
                     TVKHKLIEEIIVVSIIILIFLWHFPSSIVPIITIPISVALAFIPMKAMGLNANLMSLA
                     GIAISIGVLVDGAIVEVENAYNKIYHWIRDGKKGDFYHVRLEALMEVGPGVFFSLLVI
                     AVAFMPIFTLVDQEGRLFRPLAFSKNLTMAIAAVLAITLDPAMRMLFARVEPYTFRPR
                     FLSWLFTQAFVGKYYSEERHPISRLLHYLYETPCRFVVRHAKATLFVAALIVATTIPV
                     YAKLGSEFMPPLREGTILYMPSAVQPGMSVAEAQKALQIQDKILMTFPEVLRVFGKAG
                     RANTSTDPAPFTMMETTILLKPESEWRERPRWYSSWSPEWLKPALRSFWSDRISHGEL
                     EDEMNNALQLPGISNAWTMPIKGRLDMLSTGIRTPIGIKVMGADLETIERIAKQTEAA
                     VARIPGTRSAYAERVAGGYFLDFVLKRDKLARYGLSVDDANMMVMTAVGGDNQSVTVE
                     GRERYGINVRYARDYREDLSALQRVLLPLPSGNGQIPMQEIADVTLTQGPSMIRDENG
                     LLAGYVYVDFDTNQIDIGHYVDQAKHAVAADVRVPPGYSLTFSGQYENMQRVRERMKL
                     VLPLTLVLIFALLYMNTKSAFKSAVVMLAVPFSAVGAVWLLYALGYNMSIAVWVGLIA
                     LMGLDAETGVFMLLFLDLSHQEFKQQGRLNTQRDLVEAIIHGAVKRVRPKAMTVSAAL
                     LGLLPIMWSTGTGADLMKRIAAPMVGGLVTSFLMELLVYPAIYYLWRKRELPVANALK
                     PS"
     gene            complement(5730..7145)
                     /locus_tag="VFQ61_36770"
     CDS             complement(5730..7145)
                     /locus_tag="VFQ61_36770"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HET9960120.1"
                     /translation="MKYLLSRRSSLHRVVRLGWGITSSLVLAASIAVACGGDDDDGTG
                     ATGGARASGGSSSGGAQSGGAGQGGTRPQTGGGGGAGDQTGNQSGGAETGGSESGGRE
                     SGGSAGVGTGGEDSITGGSGGAGGGEATGGAQQQSVIEGDRRIPFSPPDDRAFVDFFI
                     QHHHMAIDIAVQEALHGEREDIKELADDLSKKQTNEVELMEALRRDTQGTALPNPPLD
                     PHADAALNQVLSLEGSDLDKLFLTEMIALHAEALPVIHRAMHALTRTDLKSLAADMFQ
                     AEAEEIGTMQGLLAELGVTDAGEDFATSTSNRPDFGLRGDLRIPLTPANDVQFIDFFA
                     PHHGMAIEMADHVAAHGSDSEVKVLAAAMSEAQSLEVKALQMVRETLTSNPDSPAPPT
                     DEYMKDAMDEMMSLEGAELDRMFLMEMIPHHASAIPTAHRAKPHVTNTALRNMSNAIF
                     HKQAAEIGVMARLLVKVSEKK"
     gene            7572..8588
                     /locus_tag="VFQ61_36775"
     CDS             7572..8588
                     /locus_tag="VFQ61_36775"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HET9960121.1"
                     /translation="MSRSPAIRSALEQRHLALEAVLRHGWNATAFQTLNNEFKYFFSQ
                     DACVAYVDTGSAWVVAGAPIAATERLREVTQMFLAEARSLGRRCCFFGTEPRFLKAAA
                     GFTRSLSIGEQPVWNPQLWPETLARHASLREQIRRARAKGVMTRALDPGESIHEVIRA
                     TRELAESWSATHAMPPMGFLVRVNPFSFPEQRRCIVAERAGRVLAVAHLVPVPKRAGW
                     FIEHLLRAPDAPNGTTELLVHAAMTSTRSEGCRWLTLGLAPLSGEVSPVLRLARQHMR
                     FLYDFEGLRRFKAKLRPSSWQPIYLTHPVQQSAWLTLRDVLAAFADDSFLRFGARTLW
                     RGLR"
     gene            8656..10047
                     /locus_tag="VFQ61_36780"
     CDS             8656..10047
                     /locus_tag="VFQ61_36780"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HET9960122.1"
                     /translation="MDLDLTAAQVQALAPDASSAVTAKKIAKAALWKSLGASPDALWG
                     EFQGSALYQTQVARLDWSMRCSCPSRKLPCKHALGLLMIAAQSPGALPATSEPEWVRS
                     WLERRAAAQTRKETRKNSEPKPVDAATQAKRAEGQAKRAEKRQDNILGGLDQLDAWLN
                     DLVRQGLARIQGESFVFWDEQARRLVDAQASGLASRVRQISYRVGAGENWAARVLDDL
                     GLLALLSHAYRRVNELDAPLAADVRRLVGLNLDQDDVLEHADLTRDTWAVLAERSEED
                     ERLRTQRAWLRGKNSARTALLLQFAPAGSRFAESLSVGTEFAAELAFWPSAFPQRALI
                     AQRLGLATPAEAPRGQSVTRCLDAFSLNVSRLPWLERDLFVLEKVIPTQSVVPHQSGE
                     QASSVPNTSSERYVVVDLEQRAIPLVGSRHDILLALSGGHPLLLIGEWNGYTLNPLVA
                     WSEGRCHNLQVPE"
     gene            10044..11579
                     /locus_tag="VFQ61_36785"
     CDS             10044..11579
                     /locus_tag="VFQ61_36785"
                     /inference="COORDINATES: protein motif:HMM:NF039502.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HET9960123.1"
                     /translation="MSSAPGADPDLLRVAILGTDQAPSSPSERFAPPIDEVLEALPGL
                     ERERRLLLSAAGYALLRRTARAPETQPLPTQVAADEILHPPSAKLRQLLTQLLEDGSL
                     ELLLEAFARMRLASLRLPEELLPRALGVSHAALREALRPVLGERGHWLAMLKPEWAWA
                     RDAGTGLAFSEAELEARFLDAAGAERKALFLVARRAAPELSRAWLSTYWRQEKADQRL
                     AWLEVLAGDVTAADEAVLSSMLNDRSAPVRMAAARLCWSIPSSELAIRLRARADALLT
                     LAEPSSGESSLRVALPPATFDPQLEREGVVESPPQGTGRRQWWLAQILAAVPPAHFQT
                     RFRAAPEQLVQAAQEHELKTALLDGFTAAALRHESQAWYAPLWDAAIRTGALSSNSNY
                     RLVTDPIAALSPRLRAEEAAPRMLALIEGGASDRLQHFARPWPNSISLIFLKALTTYQ
                     PAFTPLLPRAALALPLELLPDSFPVPEVPENQYAVQAYLRALDQFQATVAIRRTIAQE
                     TAP"
     gene            11576..12679
                     /locus_tag="VFQ61_36790"
     CDS             11576..12679
                     /locus_tag="VFQ61_36790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET9960124.1"
                     /translation="MTESIASMASAALRAHAEREYAEELAELAKHDNRPRPPNWKLSP
                     WAVRTYLMGGELENGFRVTPKYVGNARLIEIAIATLATDRALLLYGLPGTAKSWVSEH
                     LSAAISGDSTLMVQGTAGTDETALRYGWNYALLLAKGPSEAALVPSPMLRAMREGRLA
                     RVEELTRIPSEVQDTLITILSEKTLPVPELDSEVQAQRGFNVIATANNRDRGVNELSS
                     ALTRRFNTVVLPVPETLDEEVSIVDRRVRDMGKALELPVEKPALEEIRRVVTVFRELR
                     EGKTVDGKTKLKTPSGTLSTAEAISVVTAGLAMAAFYGEGVVTANDLASALSGAIIKD
                     PVQDKLIWQEYLQTVVKERDGWKDLYRASREVT"
     gene            12681..15002
                     /locus_tag="VFQ61_36795"
     CDS             12681..15002
                     /locus_tag="VFQ61_36795"
                     /inference="COORDINATES: protein motif:HMM:NF040143.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HET9960125.1"
                     /translation="MTLHVFGVRHHGPGCARALRAALEQLAPDIVLVEGPPDANETLT
                     LLSNERLRPPLALLVHAVDDPQNSSFYPFAEFSPEWQALSYAAARRIPARFMDLPCAN
                     SLAIRGARARDSDSPENDHAESVGPNGPQPTEDTDSIQGPSVALPERLEALRDDPIGI
                     LAEAAGFSDHEQWWDVQVEQRQASTGLFEALLEAMKALREGRVEPRRERQREAHMRQT
                     IRAAQKEGFQRIAIVCGAWHAPVLAELGPAKPDLELLKGLPKTKVAATWIPWTYSRLS
                     FRSGYGAGVDSPGWYGHLWEHGEKAAVVWATLAARLLRGEDLDASAANVIETVRLADT
                     LASLRELPNAGLTELREAIEAVLCGGDKTRLSLIRNRLEIGDALGEVPDGVGQVPLMR
                     DFEREAKRLRLKLTTQIIELDLDLRKDNDRDRSRLLHRLNVLGVEWGKPASSRQGMGT
                     FREGWEVAWQPELAVDLIAANLRGNTIELAATRALTERAGAADLPELSRLLELALIAH
                     LPASLDTLLHELDARAATSSDVRLQMEAILPLSRLVRYGDVRATRAELVLPVLKALFE
                     RIVVGLSAACTQLDDDAAAQMVAAIGQAHSACSLLDDAELKRDWLSALRDALDKSAAH
                     ARIRGRVCRLLIEQQELAEPELAERASLALSPGVEPAEASRWVEGLVAGEGLSLVHQE
                     QLLGSLDAWLQTLDDSVFQAQLPLLRRAFSGLTGPERRAVAQRVKHGPKRGRARPVHD
                     QFEPERAARIIPIFAALLGVTYAAARPNGEPQT"
     gene            14999..16132
                     /locus_tag="VFQ61_36800"
     CDS             14999..16132
                     /locus_tag="VFQ61_36800"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET9960126.1"
                     /translation="MSDRLERWRLVLGQAAEPELGRELSGQLLGMDRTLEALYDSDRQ
                     GGLGASSPNVARWLGDIREYFPASVVKLMQQDALDRLNLRQMLLEPELLGQVEPDVHL
                     VANLLALRSVIPARTKETARQVVRRVVEELERKLKNPMHQAVRGALQRSQRTARPRPN
                     EIDWHRTIHKNLRHYSAEHGSVVAERVVGFARRRAALRDIVLCVDQSGSMATSVVYSS
                     VFGAVLASLRSVSTRFVLFDTAVADLTDNLRDPVDLLFGAQLGGGTDIQRALTYCQQV
                     TLRPAQTILVLITDLYEGGDSQRLLARAAELKTSGVHLICLLALNDQGAPSYDHENAR
                     ALAALGIPAFACTPDLFPELMAAAIQRQDVTLWAARHNIVLGS"
     gene            complement(16340..16855)
                     /locus_tag="VFQ61_36805"
     CDS             complement(16340..16855)
                     /locus_tag="VFQ61_36805"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9960127.1"
                     /translation="MRSAFDALELRLTLRIRRCVRRDLLNLEWFGMFTHDKQVIRRTY
                     RQQALGKQEMLVADIGGFPVGQIWVQLPGNCAHPAGYLWALRVFPVLQGQGIGTRLLR
                     NSERLLLARGATHAEIIVDRSNTRAKKLYESLGYRTVVVTDSLSAPARESYGPSSEQW
                     LLSKRLLCSAP"
     gene            complement(17111..>17666)
                     /locus_tag="VFQ61_36810"
     CDS             complement(17111..>17666)
                     /locus_tag="VFQ61_36810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="calcium:sodium antiporter"
                     /protein_id="HET9960128.1"
                     /translation="WMVREGRRASAIARAGRDVESALEAADAAGAPHEAGKLRAGVTA
                     VIGIVVLLLGGNQFVKGAIAVATAMGMSERLVGLTVVAVGTSLPELVTSVIAAKRGHS
                     DLAVGNVVGSNIFNVFLCLGSAGLVGNIAISMRAAAIDLVALVAMTGFAALLIRRERT
                     SSRLEGGFALGLYLLFAVLTIARD"
BASE COUNT         3285 a         5833 c         5396 g         3152 t
ORIGIN      
        1 ccgagagcgc aacgcttgcg gaacggaatg cctatcccga cctgacggtc ggggctggaa
       61 tcatgtatcg cggtgccctg ccaccgatgt ggctcgtgac gctcggagcc cccgtcccga
      121 tcttctcggg cgctcggcag agccacgcgg cagccgaaag tcgcgcttgg gcgtctgccg
      181 cgcagagcca agtcgcagag ctcgagcaat tgcttcgact gcgcgcacaa gaacgcgagc
      241 tcgccttcgg cgccacactc gatatgttgg ccgtctacga ccagggcctg ctggttcaat
      301 cgcaggccac ggcggagagc gcgctcaccc aatacaaagt gggtaaaggc agcttcagcg
      361 ccgtactcga ggcccaagcc ggcttggtcg cggatcaaga aggttatttg gctctgctcg
      421 cgaacgctca ccgcatcctg atcgcagagg ccgaagtcag cctggccgac accgccattc
      481 ctagcggcgg cgcggcttct ggcggcatgc ccggagcaag cgccaaaccc atgaacgcac
      541 ccgccatgag ctccacggct ggcgcaagca cctcccccgc cgctgccccc agcaccgcgg
      601 gctcatcctc cggaatgtga tcgtcatgac cagtcgagag ccacctcctc ccgagtcacc
      661 cctgctcacc gagcctccgc tcggcgacac ccccgccaag gcggaagccc gaccccgcac
      721 ggggtacagc ggctgggcgc tcctcatcgt aggcgccctg agcggtgtcg tcggctccgc
      781 cgcgaccttt ctcgcgctgc agcagcttgg atcgggctcg cccttcaagc acgagcacgc
      841 gtcggaagcc cctctgtacc agtgcccgat gcacccaacc atcatccaag atcatcccgg
      901 caattgcccg atctgcggca tgaagctggt gaaggtcgag aagagcaaag ttcaaaatag
      961 tgtcgcctcc gcaccgggga atgccagcgc aacccatggc gccgctggtt ccggaccagg
     1021 aactgccagc tcgacaccaa cgcccagtgt gcctgggctc gccagcgtcg aaatcgatcc
     1081 cgagcgacaa cagttgattg gccttagaac ggccgaagtg acgcggggcc ccgtgggcgg
     1141 agcctggcga accgtcgggc gcgtggcaat tgacgaaacc cgggttcggc acgtcaacct
     1201 gaagatcccc ggcttcgtgg agcgcattta cgtcgactac atcggtaaga aagtcggcaa
     1261 aggggacccg ctgttctcga tctacagccc ggagctgctc tccgcgcagg aagagtatct
     1321 gctggcccgc aagactcaaa cggacctctc caagatgggg ggccctcttg caccgagtga
     1381 cgacggcatg gtggccgccg cgcgccgcaa actcgagctc tgggacatat ccgcagcgga
     1441 gatggacaac ctcgtgcgca cggggcaacc gaagaagagc atgacgctct actctcccgc
     1501 gtccggcgtg gtcaccaaga aggacgtggt cgagggcatg aagctcgacg ccggagcaat
     1561 gccttacgag atcgtcgatc tatcttcggt ctgggtgttg gcggacgttt acgagagtga
     1621 gctacgcttc atcaaacagg gcatgtcggc caatctcacg ttggcggcat ttcccaatcg
     1681 agagttcaaa ggcaaggtcg tgttcctgga cccgctgctg gatccgcaga cccgcaccgt
     1741 caaagtgcgt ctcaccttcc cgaatccaac cggcgagctg cgacccgaga tgttcgggga
     1801 cgtggtactg ttcggcgcgg cccacgaggg gctgcgcgtt ccgcccgacg ccatcatcga
     1861 ttctgggatc gagaaggtgg tgttcgtcgc ggtgggcgca ggcaagttcg agccgcgccg
     1921 ggtccaagtc ggcaacggcg atgagacctt cgtggaggtc gtgagcggcc tggagccggg
     1981 agagcgcgtc gtggtccgcg caaacttctt gatagactcc gagtcccgct tacgagcgtc
     2041 gttgtccgcg gtcggccgag aagaaaaccc gatccctcca gccgagagcg cacgagtgct
     2101 ccccggctct gttaatccga acgctgttaa tccgaacgct gcagccgcga acacggccgc
     2161 agccgcgagc ggcgccggta cacccaaagc caatgctgcc cccggcgcgg tcgcaccggc
     2221 caaaccccct caaaatgccc ctggcaaagc ccaaaccgtg gctcccagcc cgaacaaggc
     2281 cgcgcaagac cacagcgccc acacgggtca cgatcacagc gggcacatgc catgatcaag
     2341 gcaattatcc gcttctccgc cgagaatcgc tatttggtga tcgcggcgac gatcgtggcg
     2401 ctcgtgtttt cctggtggtc gatgcgccag atcccactcg acgcgctgcc cgatctctcg
     2461 gacacgcagg tcatcgtgta cgggcgttgg gaccgtagcc cggatatcat cgaagatcag
     2521 gttacatatc caatcacgac ggccctgctc ggcgccccca aggtcaaagc catccgcggc
     2581 ttctccgatt tcggcttcag ctacgtttac gtgatcttcg aggacggcac ggatatgtac
     2641 tgggcacgca ctcgtgtgct cgaatatctc tcgaagataa cctcacagtt gccagcggga
     2701 gtcaaagtcg agctcggccc cgacgcgtcc agcgtcggct gggtctttca gtacgcgctg
     2761 ctagacaaaa ccgggaagca ctcttcggat gagctccgct cctatcaaga ctggtttctc
     2821 cgttatgcgg tccagagcgt gcctggggtc tcggaagtgg ccaccgtggg cgggcaagtc
     2881 cgccaatacc aggtcaccgt caatcccaac tcgctggcag cctacaagct cccgctcgaa
     2941 tcggtgatcg acgccgtccg caaaggaaat aacgacgtcg gcggccggct cgtggagctc
     3001 tcgggccgcg agtacatggt gcggggccgt ggctacgtga agaacattca cgacatcgaa
     3061 gacatcgtat tgcgctccgg cgcgggcacc cccgtgcgca tcaaagacgt cgcgagcgtc
     3121 gccctcggtc ccgaaatgcg acgcggcatc gccgattaca acggtcaagg ggacgcggtg
     3181 ggcggcatcg tgatcatgcg ccaaggggag aacgcgctga acgtgatcga gcgcgtgaag
     3241 accaagctcg acgagctcaa gccctccctc cccccgggag tcgaggtggt caccacctac
     3301 gatcgctcgg aactgatcga gcgcgcgatc gataccgtca aacacaagtt gatcgaagaa
     3361 atcatcgtcg tctcgatcat cattttgatc ttcctgtggc acttcccgtc gtcgatcgtg
     3421 cccatcatta cgattccgat ctcggtcgcg ttggcgttca ttcccatgaa agccatgggg
     3481 ctcaacgcga acctcatgtc gctcgcggga attgcgattt caatcggcgt cttggtcgac
     3541 ggcgcgattg tcgaagtaga aaacgcctac aacaagattt accactggat ccgcgacggc
     3601 aagaagggcg acttctacca cgttcggttg gaagcgctga tggaggtcgg cccgggcgtg
     3661 ttcttctcgc tgctcgtgat cgcggtcgcg ttcatgccga tcttcacgct ggtcgatcaa
     3721 gaagggcggt tatttcgacc cctcgccttc tccaagaacc taaccatggc gatcgccgcc
     3781 gttttggcga tcaccctgga tccggccatg cgcatgctct tcgcgcgggt cgagccgtac
     3841 accttccggc cccgcttctt gtcttggctc ttcacgcaag ccttcgtcgg taagtactat
     3901 tccgaagagc gccacccgat cagtcgctta ctgcactacc tctacgaaac accgtgtcgc
     3961 ttcgtcgtgc ggcatgccaa agccaccttg ttcgtcgccg ccctaatcgt cgcgaccacc
     4021 ataccggtct atgcgaagct cggctccgag ttcatgccgc cgctgcgcga gggcaccatc
     4081 ttgtacatgc cctcggcggt tcaacccggc atgtcggtgg ccgaggcgca gaaggcgctg
     4141 cagatccagg acaagatcct gatgactttc ccggaggtgc tgcgcgtctt tggcaaggcg
     4201 ggccgcgcca acacctccac cgatccggcc ccattcacga tgatggagac caccatcctg
     4261 ctcaaacccg agagtgagtg gcgcgagcgg ccgcgttggt actccagctg gtcccccgaa
     4321 tggttgaaac ctgcgctaag aagcttctgg agcgatcgca tcagtcacgg cgagctcgag
     4381 gacgagatga acaacgccct ccagctgccc ggcatctcga acgcctggac catgcccatc
     4441 aaggggcgct tggatatgct ctcgaccggc atacgcacgc cgatcggcat aaaggtcatg
     4501 ggcgcagacc tagagacgat cgaacgcatt gccaagcaaa ccgaagcggc ggtcgcccga
     4561 attcccggca cgcgcagcgc ctacgcggag cgcgtggcgg gcggctactt cctcgacttc
     4621 gtgctcaagc gcgacaagct ggctcgttac ggtctctcgg tcgatgacgc caatatgatg
     4681 gtgatgacgg ccgtgggcgg cgacaatcag agcgtgaccg tcgagggacg cgagcgttac
     4741 ggcatcaacg tgcgttacgc gcgggactat cgagaagacc tgagcgcact ccagcgcgtc
     4801 ctgctaccgc tgccgagcgg caatgggcag atcccgatgc aagagatcgc ggacgtgacc
     4861 ctcacgcaag gtccgtccat gatccgcgac gagaatggct tactggctgg ctatgtctac
     4921 gtcgacttcg acacgaacca gatcgacatc ggtcactacg tggatcaagc caaacatgcc
     4981 gtcgccgcgg acgtgcgcgt gccccccggc tactcgctca cgttcagcgg ccaatacgag
     5041 aacatgcagc gcgtgcgtga gcgcatgaag ctcgtgctgc ccctcactct ggtgttgatc
     5101 ttcgccctgc tctacatgaa caccaagtcc gccttcaaat ccgcggtggt gatgttggca
     5161 gttccgttct ccgcggtcgg agcagtgtgg ctcctttatg cacttggcta caacatgtcg
     5221 attgcggttt gggtcgggct catcgcgttg atgggcctgg acgcggaaac tggcgtattc
     5281 atgctgctgt ttctcgacct ttctcaccag gagttcaaac agcagggccg cctcaacacg
     5341 caacgggatc tggtcgaagc catcattcac ggcgcggtca aacgggttcg cccaaaggca
     5401 atgaccgtat ctgcggcgct gctcggcttg ctccccatca tgtggtccac cggcaccggc
     5461 gcagatctca tgaagcggat tgctgcgccc atggtcggcg gcttggtcac gagcttcctc
     5521 atggagctgc tcgtgtatcc cgcaatctat tacctgtggc gcaagcgaga gttacccgtc
     5581 gccaatgcgc tgaagccttc ctgaacccgc aggtccttgg attcacctgc ttaccaattc
     5641 gcattcagcg accgacggtc gtgactcagt gcgactcccc ccgacgtgcg ggctccccag
     5701 agcggcccgc acgtccgagc gcgttcgaat cactttttct cggatacctt caccagcagc
     5761 ctggccatca ctccgatttc cgcagcttgc ttgtggaaga tcgcgttgga catgttgcgc
     5821 aaagccgtgt tcgtcacgtg cggtttcgcg cgatgagcag tggggatcgc cgaggcgtgg
     5881 tgcggaatca tctccatcag gaacatgcga tccaactccg ccccctcgag actcatcatc
     5941 tcgtccatgg cgtccttcat gtattcgtcc gtcggtggcg ccggcgagtc cggattggag
     6001 gtcagcgttt cgcgcaccat ctgcaaggct ttgacctcga ggctctgagc ttcgctcatc
     6061 gccgccgcca acaccttcac ctcgctatcg gagccgtggg ccgcgacgtg atccgccatc
     6121 tcgatggcca tcccgtggtg aggcgcgaag aagtcgatga attggacgtc gtttgcgggc
     6181 gtcaacggga ttctcaggtc cccgcgcagg ccgaaatccg ggcgattgct cgtgctggtc
     6241 gcaaagtcct cccccgcgtc ggtcaccccc agttccgcca aaaggccctg catcgtgccg
     6301 atttcttcgg cctcggcctg gaacatatcc gccgcgagag acttcagatc cgtgcgggtc
     6361 aacgcatgca ttgcccggtg aatcacgggc agagcctccg cgtggagagc gatcatctcg
     6421 gtcagaaaca acttatccag gtcgctacct tccagactca agacctgatt caaagccgcg
     6481 tcggcgtgag ggtcgagcgg cgggttggga agtgcggtcc cctgagtgtc gcgccggagc
     6541 gcctccatca actcgacttc gttcgtctgt ttcttggaga ggtcatccgc aagctccttg
     6601 atgtcctcac gttcgccatg cagcgcttcc tgaaccgcga tgtcgatggc catgtgatgg
     6661 tgttggatga agaagtcgac aaacgctcga tcgtctggcg gagagaacgg aattcgcctg
     6721 tcgccttcga taacggactg ctgctgagcg ccgccggtgg cctccccgcc gccagcgccc
     6781 ccggaaccgc ccgtgatcga atcttctcca cccgttccta caccggcgct cccccccgac
     6841 tctcgaccgc cgctctcgct acctccggtt tcggccccgc cgctctgatt gccagtctgg
     6901 tctccagcac cgcctccgcc cccggtttgt ggcctagttc cgccttgtcc agcgcctcct
     6961 gattgggctc ccccggatga actcccgccg ctcgcgcgag cgccgccggt cgcgccagtt
     7021 ccgtcgtcgt catcaccgcc acaggctact gcgatactcg cagcgagcac caagctggat
     7081 gtaatgcccc aacccaaacg aaccacgcga tgcaacgagc tacggcgaga tagaagatac
     7141 ttcattgcgc actcctgtgt agtcggctcc gtaggtgggc cacgcccgcc tggagcgtta
     7201 ccccaagcac ttctcgtgcc caccgcgaga ctcgagttcg tttgctcggt ccggggctgg
     7261 cggccgcaaa gtaaagagcg cgaatctcgg tgcggtccgg atgggcgcag acgagccgct
     7321 ctcatccacc cgcatcaccg cgcatgccgc ccgctagaac gagcgcgcca ttcgaggccc
     7381 ctcctcagca tatcgcgttc gagcctggct attcggccaa gccccgggag taaactaaaa
     7441 ccagcaccga atccgctcga cgaaatcgca gcctgcggca tagatcgccg cgatcttccg
     7501 tgaatccacc gcgcatactc cccgtcaccg cgaactcgac cctagcgacc ccagagcact
     7561 ccccggctcg cgtgagccga tcaccagcca tacggtccgc gctggagcag cgtcacttgg
     7621 cgctcgaggc ggtacttcga cacggttgga acgcgactgc ttttcagaca ctgaacaacg
     7681 agttcaagta cttcttcagt caggacgcct gcgttgctta cgtcgatacg ggtagcgcat
     7741 gggtggtcgc aggcgcgccc atcgcggcca cggaacggct gcgcgaagtc acccaaatgt
     7801 tcttggcgga ggcgcgcagc ttgggccgtc gctgttgctt cttcggaacc gagccgcgct
     7861 tcttgaaggc tgcagcgggg ttcacgcgat cattgagtat cggagaacaa ccggtttgga
     7921 atccgcaact ttggccggag acgctcgcga gacacgcgag cttacgtgag caaatccggc
     7981 gcgcgcgggc caaaggggtc atgactcgag cgctcgatcc gggcgagtcc atacatgagg
     8041 tgatccgcgc gacccgcgag ctggcggaga gctggtccgc aacgcacgcc atgccgccca
     8101 tgggtttctt ggtgcgggtc aatccattct cattccccga gcagcggcgc tgcatcgtgg
     8161 cggagcgtgc ggggcgcgtg ctcgcggtag ctcacttggt tccggtgccc aagcgcgcag
     8221 gctggttcat cgaacacctg ctgcgtgcgc ccgatgcacc gaacggtacg acggagctct
     8281 tggttcacgc cgccatgacc agcacccgca gcgagggctg tcgctggctg acgctcgggc
     8341 tcgcgccctt gtccggtgag gtttccccgg tgctccgcct ggcgcggcaa cacatgcgtt
     8401 ttctgtacga cttcgaaggg ctgcgccgct tcaaggccaa gctgcgaccc agctcctggc
     8461 agcccattta cctgacgcat cccgtccagc agagcgcttg gctcacgctg cgggacgtgc
     8521 tcgccgcgtt cgcggacgat agcttcctgc gcttcggcgc acgaaccttg tggcgcgggc
     8581 tgcgctgaat ggatcggaga tcgcgacggc cacgcgggcc gcgccaggtc agccgtgata
     8641 agtaggcaag cctgaatgga tttggacctc accgccgctc aggtgcaagc cctggctcca
     8701 gacgcgagct cggcggtgac ggcgaagaag atcgccaaag ccgccctgtg gaagtctctc
     8761 ggcgccagcc cggacgcgct ttggggtgag tttcaaggca gcgcgctcta tcaaacccaa
     8821 gtggcgcgct tggactggtc catgcgctgc agctgcccga gccgcaaatt gccgtgcaag
     8881 cacgcgctcg ggctgttgat gattgcggcg cagtctcccg gcgcgctgcc ggcgacgagc
     8941 gaacccgaat gggtacgcag ctggctcgag aggcgcgccg cggctcagac gcgcaaggag
     9001 acccgcaaga actccgaacc gaagccagtc gacgcggcga cccaggccaa acgcgccgaa
     9061 ggccaagcca agcgtgcaga gaagcgccaa gacaacatct tgggcgggct ggaccaactg
     9121 gacgcttggt tgaatgattt ggtgcgccaa gggctcgccc gcattcaggg cgagtccttc
     9181 gtgttctggg acgagcaggc gcgaaggttg gtggacgcgc aagcgtcggg actcgcctcc
     9241 cgggtccgac agatcagcta tcgagttggc gcgggggaaa actgggctgc gcgtgtactc
     9301 gatgacctcg ggctgctcgc gttgctcagc cacgcttatc gtcgagtgaa cgagctcgac
     9361 gctccgctcg cggccgatgt gcgacgactc gtcggcttga atctggatca ggacgacgtg
     9421 ctcgagcacg cagacttgac ccgagacact tgggccgtgc tcgccgagcg cagtgaagag
     9481 gacgaacgac tgcgcacgca acgggcctgg ctgcgcggca agaacagcgc gcgaacggcg
     9541 ttgctgcttc agttcgcgcc cgcgggcagc cgcttcgcag agtccctatc ggtagggacc
     9601 gagttcgcgg cggagctcgc gttttggccg agcgcatttc cccagcgagc cctgatcgcg
     9661 caacgccttg gcctggcgac gccggcggag gcgccgaggg gccagagcgt gactcgctgt
     9721 ctggacgcct tcagtctgaa cgtgagtcgg ttgccgtggc tagaacgcga cttgttcgtg
     9781 ctcgagaagg tcattccgac ccaaagtgtg gtcccgcacc aatccggcga acaggcctcg
     9841 tccgtaccca acacttcgtc cgagcgctat gtggtagtcg atctcgagca gcgcgcgata
     9901 ccgctcgtag gatccagaca cgatatcttg ctcgccctga gcggcggcca tccgttgctc
     9961 ttaatcggag aatggaacgg ctacacattg aacccattgg tcgcctggag cgagggccga
    10021 tgtcacaacc tgcaggtccc cgaatgagct ccgcacccgg cgcggatccg gacctgctgc
    10081 gtgtggccat cctcggcacg gaccaagcgc cgagcagccc gagcgaacgc ttcgctcccc
    10141 ccatcgacga ggtcctggag gcgctgccgg ggctagaacg tgagcgcagg ttactcttgt
    10201 ccgcggcggg ttacgcttta ttgcgtcgca ccgctcgcgc gcccgagacc cagcctctgc
    10261 ccacgcaggt tgcagccgat gagatcttgc acccgccgtc ggcgaagctg cgtcagctcc
    10321 tcacgcaact gctcgaggac ggcagcctcg agttactgct ggaggcattc gcgcgcatgc
    10381 gactggcgag cctgcgtctg ccggaagaac tgctgcctcg ggcgctcggc gtgagccacg
    10441 ccgccctgcg cgaagctttg cgtccggtgc ttggggaacg cgggcactgg ctcgctatgc
    10501 tgaagcccga atgggcctgg gcccgggacg caggaactgg gctcgccttt tccgaggctg
    10561 aactcgaggc acgctttctc gacgccgccg gggcggagcg taaggcgcta ttcctggtgg
    10621 cccgccgcgc agcgccggag ttgtcgcgag cttggctgag cacctactgg cgtcaagaga
    10681 aggccgatca acgcttggcc tggctagagg tattagcggg cgacgtgacg gccgcagacg
    10741 aagccgtgtt gagcagcatg ctcaatgatc gctccgcgcc ggtccgaatg gcggcggccc
    10801 gcctgtgttg gtcgattccc agcagtgagc tcgcaattcg attgcgcgcg cgtgccgatg
    10861 cgctgctcac gcttgccgag ccgagttcgg gcgagagcag tctacgcgtc gccctgcccc
    10921 ctgccacttt cgacccacag ctagaacgcg aaggtgtagt cgaatcgcca ccccaaggca
    10981 ccggacggcg acaatggtgg ctcgcgcaga ttctggcggc cgtccctcca gcgcacttcc
    11041 agacccgctt tagggcggcg ccagagcagc tcgttcaagc agcccaagag cacgaactca
    11101 agaccgcatt gttggacggg ttcaccgccg cggcgttacg gcatgagagc caggcatggt
    11161 acgcgccgct ttgggacgcc gcgattcgaa cgggtgcatt gagctccaat tcgaactatc
    11221 gactcgtcac cgatccgatc gccgcattga gcccccggct ccgcgcagag gaagccgctc
    11281 ctcgcatgct ggcgctaatc gagggaggcg cctccgatcg actgcagcat ttcgcacgcc
    11341 cttggccaaa ctcgatatca ctgattttcc tgaaggcgtt gacaacgtat caaccggcgt
    11401 tcacgccact gttgccgcgc gccgccttgg cgctgcccct cgagctactc cccgattctt
    11461 tccccgtacc tgaggttccc gagaaccaat acgccgtgca ggcttacctg cgggcactcg
    11521 atcagtttca agccaccgtg gcgatccgtc gcacgatcgc ccaggagacc gcaccgtgac
    11581 cgaatccata gccagcatgg cgtcagcggc gctccgcgcg cacgcagaac gcgagtacgc
    11641 cgaagagctc gcggagctcg cgaaacacga taaccggccg cgtcccccga actggaaact
    11701 atcgccctgg gccgtacgca cctacttgat gggtggcgaa ctggagaatg ggtttcgcgt
    11761 cacccctaag tacgtgggca acgcgcgact gatcgaaatc gcaatcgcca ccttggcgac
    11821 cgatcgcgcg ctgctgcttt atgggcttcc tggcacggcc aagagctggg tcagcgaaca
    11881 tctgagcgcg gcgatttcgg gagactcgac tttgatggtg cagggcaccg ccggcaccga
    11941 cgagaccgcc ctgcgttatg gctggaacta cgctttgctg ctcgccaaag gtccgagcga
    12001 agctgcgctc gtcccgagcc ccatgctacg agccatgcgc gaggggcgct tggctcgggt
    12061 cgaagagctg acccgtatcc cgagcgaggt gcaagacacc ttgatcacga ttctgtccga
    12121 gaagacgctg ccggttcccg agctcgacag cgaggtccag gcccaacgcg gcttcaacgt
    12181 gatcgcgacg gccaacaacc gagatcgggg cgtgaacgaa ctctcgagcg cgctcacgcg
    12241 ccgcttcaac acggtcgtgc tccccgtccc ggagacgctc gacgaagagg tgtcgatcgt
    12301 ggaccggcgc gtgcgcgaca tgggcaaagc actcgagttg cccgtcgaga agcctgccct
    12361 cgaggagatc cgccgggtgg tcaccgtgtt tcgcgaactc cgcgagggca agaccgtcga
    12421 cggcaagacc aaactgaaga caccttccgg cacgctctcg accgcagagg ccatcagcgt
    12481 ggtgaccgcc ggcttggcga tggccgcctt ctatggcgag ggcgtggtga ccgccaacga
    12541 tttggcctcg gcgctgagcg gcgcaatcat caaagatccg gtgcaggaca agctgatttg
    12601 gcaggagtat ctgcaaacgg tggtgaaaga gcgggacggc tggaaagacc tctaccgcgc
    12661 gagccgcgaa gtaacgtgag atgactctgc acgtcttcgg agtgcggcac cacgggcccg
    12721 gctgcgcccg cgcgttgcgg gcagccctcg agcaactcgc tcccgacatc gtgctcgtgg
    12781 agggcccacc ggacgcgaac gagaccttga cgctgctgtc gaacgagcgg ttgcgcccgc
    12841 cactcgcgct gctggtccac gcggtggacg acccgcagaa ttcgtcgttc tatccgttcg
    12901 ccgagttctc gccggagtgg caggccctga gctacgctgc cgcacgcagg atccccgcgc
    12961 gcttcatgga tttgccgtgc gcgaactcac tcgcaatccg cggcgcacga gccagagact
    13021 cggactcccc cgaaaacgac cacgcggagt ctgtgggccc gaacggcccg caacccacgg
    13081 aagacaccga ctccatccaa ggcccatccg tggctctgcc cgagcggctc gaggcgctgc
    13141 gcgatgaccc gatcggcatt ctggccgagg ccgctggctt ttccgaccac gagcagtggt
    13201 gggacgtgca ggtcgagcag cgccaagcct cgacgggtct gttcgaggcc ctgctcgagg
    13261 cgatgaaggc gttgcgcgag ggccgcgtcg aacctcgccg tgagcggcaa cgcgaagcgc
    13321 acatgcggca gacgatccga gctgcacaaa aggaaggctt ccagcgaatc gcgatcgtct
    13381 gcggcgcttg gcacgcgccc gtactggccg agcttggccc ggccaagccc gatctggagc
    13441 tactcaaagg cttgcccaag accaaggtcg cggccacctg gatcccgtgg acgtattcgc
    13501 ggctctcctt ccgcagcggt tacggcgccg gcgtggattc acccggttgg tacgggcacc
    13561 tgtgggaaca cggggagaag gccgcggtgg tttgggccac gctcgctgcg cgcctgctgc
    13621 gtggcgaaga tctcgatgcc tcggcggcaa acgtcatcga gaccgtacgt ttggcggaca
    13681 ccctcgcgtc tcttcgtgag ctaccgaacg ccggactcac cgagctgcgc gaggcgatcg
    13741 aagccgtgtt gtgtggcggg gacaagaccc gcctgagttt gatccgcaat cgcctcgaga
    13801 ttggagacgc gctgggcgag gtacccgacg gcgtcggaca agtgcccttg atgcgcgact
    13861 tcgagcgcga ggccaagcgg ctgcggctca agctcacgac ccagatcatc gagttggacc
    13921 tggatcttcg aaaagacaac gatcgcgacc gcagccgact cttgcatcgc ttgaacgtgt
    13981 tgggcgtgga gtggggaaag cccgcttcga gccgccaagg catgggcacg ttccgagaag
    14041 gttgggaggt cgcgtggcag ccggagctcg cggtggatct gatcgccgcg aatctgcgcg
    14101 gcaacaccat agagctcgcc gccacccgcg ccctcaccga gcgcgccggt gcagccgact
    14161 tgcccgagct ctcccgtcta ctcgagttgg ctctgatcgc ccacttgccg gcctcactgg
    14221 atacgttgct gcacgagctc gatgcccgag ccgcaacctc gagcgacgtg cgcctgcaaa
    14281 tggaagccat tttgccgctc tcacggctgg tacgttatgg cgacgtgcgc gccacgcgcg
    14341 ccgagctcgt cttgccggtg ctcaaagcct tgttcgagcg catcgtagtc gggctcagcg
    14401 ccgcctgcac ccagctcgac gacgacgccg cggcgcagat ggtagccgcg attggacagg
    14461 cccactccgc ctgttcgctg ctcgacgacg ccgagctgaa gcgggattgg ctctccgcgt
    14521 tgcgcgatgc gctggacaag tcggccgcac acgcccgcat tcggggccgc gtctgccgtc
    14581 tactcatcga gcaacaagag ctcgcggagc ccgagctcgc ggagcgcgcg agcctcgcct
    14641 tgagccccgg cgtagaacct gcggaagctt cacgctgggt cgaaggcttg gtcgcgggag
    14701 aagggttatc cttggtgcac caggaacaac tgctcggcag cctcgacgct tggctgcaga
    14761 cactcgatga ttccgtcttt caggcccagc tgccgctcct ccggcgagcg ttctcggggc
    14821 tcaccggtcc agagcgtcga gccgtggccc agcgggtaaa acatgggccc aagcggggcc
    14881 gcgcgcggcc cgtgcacgac caattcgagc ccgaacgcgc ggcgcgcatc ataccgatct
    14941 tcgccgcact actaggcgta acttacgccg ccgccaggcc gaacggagaa ccgcaaacat
    15001 gagcgaccgg ctcgaacgtt ggagactagt gctcggtcaa gccgcagaac ccgagttggg
    15061 tcgcgagctg agcggacaac tgctcggcat ggatcgcacg ctcgaagcgc tgtacgactc
    15121 cgaccgtcaa ggcggactag gcgcctcgag cccgaacgtc gcgcggtggc tgggggacat
    15181 ccgcgaatac ttcccggcgt ccgtggtgaa gctgatgcag caagatgcgc tggatcgcct
    15241 gaacttgcgc cagatgctgc tcgaacccga gctcttgggc caagtggaac ccgacgtaca
    15301 cctggtcgcc aatctccttg ctctgcgcag cgtcatcccc gcgcgcacca aagagaccgc
    15361 gcgccaagtc gtacgacgag tcgtggaaga gctcgaacgc aagctcaaga atcccatgca
    15421 tcaggccgtg cgaggggctc tgcaacgttc ccaacgcacc gcgcgccccc gacccaacga
    15481 gatcgattgg caccgcacca ttcataagaa cctccgccac tacagcgccg aacacggcag
    15541 cgtcgtcgcc gagcgcgtgg ttggctttgc ccgccgccgc gccgcgctcc gcgacatcgt
    15601 gctgtgcgtc gaccagagcg gttccatggc cacctccgtg gtgtactcga gcgtcttcgg
    15661 ggccgtactc gcgtctttgc gcagcgtctc cacgcgcttc gtcctattcg acaccgcagt
    15721 ggccgacctc accgacaact tgcgcgatcc cgtagaccta ctgtttgggg ctcaactcgg
    15781 gggcggcacc gacattcagc gtgcactcac ctactgccaa caagtcacgt tgcgaccggc
    15841 gcaaaccatt ctggttctga ttaccgattt gtacgagggc ggtgattctc aacgcctact
    15901 cgcacgggcc gccgagctca agacttccgg ggtccacctc atctgtctgc tcgctttgaa
    15961 cgaccaaggc gcccccagct acgaccacga aaacgcgcgc gccctcgccg ccctcggcat
    16021 ccccgccttt gcgtgcactc ccgatctatt cccggaattg atggcggccg ccattcaacg
    16081 ccaggacgtc acgctctggg ccgcacgaca caatatcgtg ctcggctctt gagggggact
    16141 aaggctcaag gcaaactcca tggcaatcgt cggatacgca gatctttcgg agagactcgc
    16201 agcaagtctg accctcgaca agtgactgtc aatcagaaca ggtttgttca acgcctcccc
    16261 cacttgctac tcggagagct cgtcaaggtt ggggccagca gcatgcggcc actatcacgt
    16321 tagataacga gaacagggac tagggggcac tacagagcag acgcttgctc agcaaccact
    16381 gttcagaact gggcccatac gattcccgcg cgggcgcaga taaactatct gtcacgacca
    16441 cggtgcgata accgagagac tcgtatagct tcttggcgcg cgtgttggat ctgtccacga
    16501 tgatttcggc gtgcgttgca ccacgcgcga gcagaagacg ttcgctattg cgtaacagtc
    16561 gagtcccaat cccttgcccc tgcaacacgg ggaatactcg tagcgcccat aggtaccctg
    16621 ccgggtgcgc gcagttgcct ggaagttgaa cccaaatttg acccacggga aatcccccga
    16681 tgtccgccac cagcatctcc tgtttaccta aggcttgttg cctgtaggtt ctccggatga
    16741 cttgcttgtc gtgggtgaac atgccgaacc actccagatt gagcaaatcg cgccgcacac
    16801 accgtctaat tcgtaacgtt agccgcagct cgagtgcatc gaaagctgat cgcatatcgt
    16861 gaaacgttgc gtcgcggcag tgctcagcca tgggtggcct ccggtgaagg aatacgctgc
    16921 gccactcagg gtgcgcgttg atcagctctg acgcaagttg cacgtggatt tttgatttgg
    16981 tgcgcacggc gagggtcgtt cagaagtgct ccgcgttagt ggtgattcaa tcgcctgctg
    17041 cgtagttgaa agacctatcc tccgcccgag cttggccacg ttgggactat cggcccttcg
    17101 aagccagcac tcaatctctc gcgatcgtaa gcaccgcgaa caggagatag agaccgagcg
    17161 cgaagccgcc ctcgagtcgc gagctcgtgc gctctctgcg tatgagtaac gccgcgaaac
    17221 ccgtcatcgc gacgagagct acgagatcga tggccgcggc gcgcatcgag atggcgatgt
    17281 tcccgaccag tccagccgat ccgaggcaga gaaagacgtt gaagatgttg gaaccgacaa
    17341 cattgccgac cgccagatcg gagtgccctc gcttggctgc aatcacgctg gtcaccagct
    17401 ctggcaggga cgtcccgacg gcgacgacgg tcaagccgac gaggcgctcg ctcatcccca
    17461 ttgcggtggc gacagcgatc gcgcccttaa cgaactgatt gccccccagg aggagcacta
    17521 cgattccgat cacggccgtc accccggcac ggagcttacc cgcttcgtgc ggcgcgcccg
    17581 cggcatccgc cgcctccagt gcggactcga catcgcgacc ggcccgagca atcgcagaag
    17641 cacggcgtcc ctctcgcacc atccac
//
LOCUS       DASTKU010001038         9481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_650660, whole genome shotgun sequence.
ACCESSION   DASTKU010001038 DASTKU010000000
VERSION     DASTKU010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9481
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650660"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..158
                     /locus_tag="VFQ61_36815"
     CDS             24..158
                     /locus_tag="VFQ61_36815"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET9960129.1"
                     /translation="MKKPATKERQGINPFTKEPITIPAKPASKKVRATALKALKDLVQ
                     "
     gene            291..1496
                     /locus_tag="VFQ61_36820"
     CDS             291..1496
                     /locus_tag="VFQ61_36820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET9960130.1"
                     /translation="MTALNVGSVTLQKGHVQPVWAGHPWVFAQAVERIEGGVTAGDEV
                     VVRDARGNALGRGLYSPGSAIPVRIYSRDPDRALDARFFEERIEEAIKRRRLLDFPSA
                     ETTGFRLLHAEGDALPGLVVDCFNDVLAVQFGTYGVKRREAWILEALQRLLSPRAIVD
                     RTTARAAKTEGFQAGEGVIRGQAELSAFEFQERRFQFSIPLSLGQKTGFYFDQRPLRA
                     RVEKLAKGCRVLDAHSYTGAVALSAARGGAASVEAVDTSALALEVAAETAERNHLLGR
                     VKFECADAQSVLNHAGNKGGYDLVILDPPKLAPSRGKRERALDAMRRLCASGARATRP
                     GGILIVSSCSAALGLPELTRAVALGARDVGLGALVLDRLFQGADHPVPAAFPEGLYLS
                     TVITQVSRL"
     gene            1505..2356
                     /locus_tag="VFQ61_36825"
     CDS             1505..2356
                     /locus_tag="VFQ61_36825"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET9960131.1"
                     /translation="MSHIPLPSLNAVPKAWLTELRGLLFDLDDTLLEHGRLLPEALQA
                     LYALSAAGYRLFAVTGRPAAWGAIITHQWPIDGAVTENGAIALCRRDGPCRSDARDRS
                     AEHAKDRRGRVVISDVLDVAARTARRARLLEIVGALSREFPELEPSDDVWQRVSDYTF
                     DIGEYRHVSADVVAKARQSAARLGASTIASSVHLHVSLDRADKATGTLRLLRELFGAD
                     PMEALAQYAFIGDSDNDAACFSAFRLTFGVRNWTGRPTLPPRFVTAEPRARGFVEVAR
                     LLLSART"
     gene            complement(2387..3712)
                     /locus_tag="VFQ61_36830"
     CDS             complement(2387..3712)
                     /locus_tag="VFQ61_36830"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET9960132.1"
                     /translation="MKRTHLSIALGAATISVSFTGCGENSNSPTMGTGGSSAGAHQNT
                     GGARSSGGTLSSGGSLSSGGALATGGSTASGGTPFGGGTSSNGGALQSGGSAAVGGTG
                     TAGSAGNSNPGGSSSGGSVNGGTSTTQGGATAGASSGGTAGGGTAGSNTGTPHGSAAK
                     FICPSGKSYGDPLSGMGQVSQFGPPASDYFAFIEGPVWIEKVGALFFSDNASSPSERI
                     FKLVPPATATAVFVKSSSSNGLAVDNEDQIVATDQDKKQIIRFDSSSGMPLGKAISTG
                     SAKPNDVLVRSDDNIYFTDPDSGFYRISPSGQLSAAMKPMQGSRPNGIELSLDENTLY
                     VGDVGNKTISQYTVAVDGSVDTASLKPFVAETKNGTVDGMCVDCAGNVYAGTSNGVEV
                     YSPTGMYLGTVPTGESSNCTFGGPDRKTLYVTSRSVLKAVTLAVPGLPD"
     gene            3804..4940
                     /locus_tag="VFQ61_36835"
     CDS             3804..4940
                     /locus_tag="VFQ61_36835"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HET9960133.1"
                     /translation="MNFTLVGVLVYAGLGDLGLGSFRPPPANAAPPGTGFWRGAGFGL
                     AMTCGVIGAAPGDAWGTYSITAFDRESGALGGAGASCVPYAVDVIYGVVPGRGAFNMQ
                     AWLDDALHADALALVAEGKSAAELLRAVTDNTAYPNAQRMQWGVVTSAGDALAWSGSA
                     ASPYAGHQLLGEDSRYTATFQGNLLTSDRVLIQARAAFSNAGCDLAERLMLSLEAAGV
                     QGEGDRRCTPDGRPANSAYLDVTLPEGPWLHLSVPDVAPSDPIVVLRALFEQFRSKHP
                     CPSASEAGAPPSQADAAPGPIRGPEASTRTTGPGCSVAMPTRGGAATHSARSSQVSTP
                     ALLWAGWGALLSLLLRLNVRRWRTTLRRSRVDRSAPRAPISSDG"
     gene            complement(4855..5196)
                     /locus_tag="VFQ61_36840"
     CDS             complement(4855..5196)
                     /locus_tag="VFQ61_36840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960134.1"
                     /translation="MTRVALERRRRFEILLPDRYNDGRAIMHECMECVPKTLMDVLNR
                     FGALSYNPHSIQGVWTHEGTRYEDRLFRLTVDTEDNAASFEFIHHLKSELEARFDQLE
                     IYVVSFSICVH"
     gene            complement(5227..5793)
                     /locus_tag="VFQ61_36845"
     CDS             complement(5227..5793)
                     /locus_tag="VFQ61_36845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960135.1"
                     /translation="MKQVCFQPIRRLGTFSLIALGTLALSTSGQTRPLPGAALEDAAP
                     DPMGATHDASTSGPTCRPVRGTFTVETSAGPDCPSAVGLCGRVEWRGDLNATSTFVAT
                     SSLETIDSPSTSVLVFTGDAEVSLRGGTLRTKDAIVFRLTGSGDFAEVDTIVGGTGVF
                     ANASGAWRASGTFQGNLGQGRYDGTICR"
     gene            6062..7276
                     /locus_tag="VFQ61_36850"
     CDS             6062..7276
                     /locus_tag="VFQ61_36850"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HET9960136.1"
                     /translation="MPSSIFSQIPLAPADPILGLTEAFQADTNPKKVNLGVGVYQDAQ
                     GKVPVLRVVREAERRWYEKEESKTYLPIDGVPAYNKAVAGLLFGNDSPLIAAGRVVTA
                     QALGGTGALKLGADFLRRFLPEAELWLSRPSWENHRAVFESAGFKVNEYPYYDPKTNG
                     LDFAAMRASLNQLPARSVVLLHACCHNPTGVDLEPAQWEEIVQIVKARGLMPFLDFAY
                     QGFGTGIAEDAQALLAFQKADIPFLVASSFSKSFSMYRERVGALSIIATDGEEARRVQ
                     SQLKRVIRTNYSSPSSHGAQVVALILSDPELRAGWESELTEMRERILRMRSSFVEQLS
                     SKGVTRDFSFINDQRGMFSYSGISLEHVRRLRSEFGLYIVDSGRVCVAALNEKNLGYV
                     TDSIAQVLGASA"
     gene            7273..8505
                     /locus_tag="VFQ61_36855"
     CDS             7273..8505
                     /locus_tag="VFQ61_36855"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HET9960137.1"
                     /translation="MSKETQDALVALGQRYLYPNYRQPGLVMQSGKGALLWDVAGKRY
                     LDFYGGIAVSALGHAHPALIAALSEQAARLIHVSNYFYNETNVRLAEKLCKATGFDRV
                     FFANSGTEAIEACLKLARRHFFMKGEPERLRVLAFHNSFHGRTLGALAATGQDKYKEG
                     FGTLPHTVHVPYGDLSAVKAAMGRDVAAILVEPIQGEGGVSPAPAGFLRELRRIADET
                     GALLIADEVQTGIGRTGKMLGIEHDDVRPDVVALAKGLGGGVPIGAMLCREALKDALP
                     PGSHGSTFGGNALASAAALAVLETIERDQLLVRTAAVGEYLAQGLLAVSREFPALVET
                     SRGRGLLQALVLRGNVDARAVLQKLWDAGLLLTIAGGQALRFSPPLIVSDAEIDEALA
                     VIRRVFASLSPSAAETGR"
     gene            8502..>9481
                     /locus_tag="VFQ61_36860"
     CDS             8502..>9481
                     /locus_tag="VFQ61_36860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960138.1"
                     /translation="MNAARATSDPSKLPCATCGSDVDPLRADRVALFGERFRYFCSVA
                     CHERYEPRVLLTPLPAPRERPSQELRAVPIEATVHVEPAELREIRRITHSEDSEPEPV
                     ASSANAELESLQGLEERGAVPGMGEGTASAIGALLLALSSIGGGLAVALSLAGNSNVA
                     VTARLVLTAVSACALAAETFLGVREETEQSPLARLAAPVGAVLLAIFAYVSASESTNG
                     IVTLAGLLVASSALSVLLVRRVRRPVDVERTALGAELDGTCKRVVLDEELAETRAVDL
                     RPGEEIVLDSGDVVPVDATLTAGSAVVLPWRGASVRAERHEGDVLVAGARV"
BASE COUNT         1559 a         2962 c         3133 g         1827 t
ORIGIN      
        1 cggggctggt caagctccgc gtcgtgaaga agcccgccac gaaagagcgt cagggcatca
       61 atcccttcac caaagagccg atcaccatcc cggccaagcc cgcgagcaag aaagtgcgcg
      121 cgacggctct caaggccctc aaggatctgg ttcagtgatc tgctgattgg ttcggcaccc
      181 gctgagccaa tcgtctcgga tcgcaagatc cgttagctcg tccccggatc tacccggaag
      241 atccggggat agcttttttg tgctggttgc gttagccccg ggaggttttc atgacagcgc
      301 tcaatgtggg tagcgtcacc cttcagaagg ggcacgtgca gccggtttgg gcgggccatc
      361 cctgggtgtt cgcgcaagcc gtggaacgca tcgagggcgg agtcacggcc ggggacgagg
      421 tcgtagtacg cgatgcgcgt ggcaacgcgc tggggcgtgg cctctattcg ccgggttcgg
      481 ccatcccggt ccgcatctat tcgagagatc ccgaccgcgc tttggatgcg cggttcttcg
      541 aggaacgcat cgaggaggcg atcaagcgcc gccgcttgtt ggactttccg agcgcggaga
      601 cgaccggctt tcgcctgctg catgcggagg gggacgccct gccgggcctg gtcgtggact
      661 gtttcaacga cgttttggcg gtccaattcg ggacgtacgg ggtgaagcgc cgggaagctt
      721 ggatactaga agctttgcag cggctgctct cgcctcgagc catcgtagat cgcacgaccg
      781 cgcgcgccgc caaaaccgag ggatttcagg ctggcgaggg tgtgatccgg gggcaggccg
      841 agctgagtgc cttcgaattt caggaacgcc ggttccagtt ctccattccg ctcagcctgg
      901 gccagaagac aggcttctat ttcgatcaac ggccattgcg cgcccgcgtc gagaagctcg
      961 cgaaaggctg ccgggtgctc gacgcgcact cgtatacggg cgcggtggcg ctctcggccg
     1021 ctcgcggtgg cgctgcctcg gtggaggcgg tcgataccag cgcgttggcg ctcgaggtcg
     1081 cggccgagac ggctgagcgg aatcacctgc ttgggcgcgt taaattcgag tgcgcggacg
     1141 cccaaagcgt cttgaatcac gccggcaaca agggcggcta cgatctagtg atcctcgatc
     1201 cgccgaagtt ggccccgagc cgcggcaaac gcgagcgagc cttggacgcc atgcggcggc
     1261 tctgcgcctc cggggctcgc gcaacgcgcc ctggtggtat cctgattgtc agctcgtgtt
     1321 cggccgcgct gggcttgccc gagctgaccc gcgccgtggc gctcggtgct cgcgacgtgg
     1381 ggcttggggc cttggttctg gatcgcttgt tccaaggcgc cgatcatccc gtgccggcgg
     1441 ctttcccgga agggctgtac ttgagcacgg tgatcactca ggttagccgc ctctagacgg
     1501 tcccatgtct cacatcccgc tgcccagctt gaacgcggtt cccaaagcct ggctcaccga
     1561 actgcgtggg ctgttgttcg acttggatga caccttgctc gagcacgggc gcttgttgcc
     1621 ggaagccttg caagcactgt atgcgctgtc cgccgcgggt taccgcttgt tcgcggttac
     1681 gggacgcccg gcagcttggg gggcgatcat cacgcaccag tggccaatcg acggcgccgt
     1741 aaccgagaat ggtgccatcg cgctgtgtcg acgggatggg ccgtgtcgaa gcgatgctcg
     1801 tgatcgaagc gcagagcatg caaaagaccg gcgcggacgg gtggtgattt cggacgtgct
     1861 cgatgtcgca gcacgcacgg cgcgacgggc tcgcctattg gagatcgtcg gcgcgttgag
     1921 tcgcgagttc cccgaactcg agccgagcga cgacgtgtgg caacgcgtga gcgactacac
     1981 gttcgatatc ggcgaatatc ggcatgtctc ggccgacgtc gtggcgaaag cgcgtcaatc
     2041 cgcggcgcgg ctaggtgcgt ccacgatcgc ctcgagcgtc cacttgcacg tgagtttgga
     2101 tcgcgcggac aaggctaccg gcacgttgcg cttgctgcga gagctctttg gcgcggaccc
     2161 catggaggcc ctcgctcaat atgcgttcat cggcgatagc gacaacgacg cggcgtgttt
     2221 cagtgctttt cgactgacct tcggggttcg caactggacg gggaggccga cgctccctcc
     2281 gcgtttcgtc acggccgagc cgcgcgcgcg cggcttcgtc gaagtcgcgc gcctactgct
     2341 ctcggcccgt acctagcatc ctccccgttg gagcgcaccg accctatcag tcaggcagac
     2401 cgggcaccgc tagcgtaacc gccttgagaa ccgaacgcga ggttacgtac agcgttttcc
     2461 gatcggggcc cccgaacgtg caattggagg actcaccggt cggaacggtg ccgagataca
     2521 tccccgtggg tgagtagact tcgactccgt tggaggtgcc ggcatacaca ttacccgcgc
     2581 agtcgacgca catcccatca acggtgccgt tcttcgtttc ggccacgaac ggcttcaagg
     2641 atgcggtgtc caccgagcca tcgacggcca ccgtgtattg gctgatggtc ttgttcccga
     2701 cgtccccgac gtacaacgta ttctcgtcga gggatagctc gatgccgttc gggcggctcc
     2761 cttgcatcgg cttcatggcg gcgctgagct gtccgctcgg tgagatgcga taaaagcccg
     2821 agtcaggatc ggtgaaatag atgttgtcgt cactcctcac cagcacgtca ttgggcttgg
     2881 cgctcccggt ggaaatggct ttgcccaggg gcataccgga gctggaatcg aaacgaatga
     2941 tttgcttctt gtcctgatcg gtagcgacga tctgatcttc gttgtcgacc gcgagcccgt
     3001 tactgctgct cgatttcacg aacacggccg tggcggtggc gggcggcacg agcttgaaaa
     3061 tgcgctcact tgggctggag gcattatccg agaagaacag agcgcccacc ttctcgatcc
     3121 agacgggccc ttctatgaat gcaaagtagt ccgatgctgg cggcccgaat tggctcactt
     3181 ggcccatgcc gctcagcggg tcgccgtagc tcttgccgct cgggcaaatg aacttggccg
     3241 cactgccgtg tggagtacct gtgttgctgc cagcagtgcc tccgccggca gtgccccccg
     3301 aagacgcgcc ggccgtggcg ccgccttggg ttgtgctcgt tccgccgttc acgctgcccc
     3361 ctgacgagct tccgccggga ttgctgttcc ccgctgatcc cgcggtgccc gttccgccga
     3421 cagccgcgct tccaccgctc tgtagtgcgc cgccgttgga gctggttcca ccgccaaatg
     3481 gtgttccacc actcgccgtt gaacctcccg tcgcgagagc tccgccggaa gacaacgagc
     3541 cacccgagga taaggtacct ccgctagacc gtgcgccgcc cgtgttttgg tgcgctcccg
     3601 cgctactgcc accggtcccc atcgtgggac tattggaatt ctcaccacag ccggtgaaag
     3661 agacactgat ggttgcggcg cccagcgcaa ttgaaaggtg cgttctcttc atgagttctc
     3721 cagcacctgg attcgactga cacggagctc gcaccgagtc gagcgcgtag acaattttct
     3781 accctcgcaa gtcggctgca aaagtgaact tcaccctagt cggggtgcta gtctacgccg
     3841 gattgggtga cttaggcctg ggttcattcc gaccgccccc cgcgaacgcg gctccacctg
     3901 gaaccggttt ctggcgtgga gcaggctttg gcttggccat gacttgcgga gtgattgggg
     3961 ctgcgccggg tgatgcttgg gggacgtact cgatcactgc tttcgaccga gaaagcggag
     4021 ctttgggcgg tgctggcgct tcttgcgtcc cttatgcggt cgacgtcatt tacggtgtgg
     4081 tccccggacg cggggctttc aacatgcagg cctggctgga cgatgcgttg catgccgacg
     4141 cgttggcgtt ggtcgccgag ggaaagagcg ctgccgaatt gctgcgggcc gtgacagaca
     4201 ataccgcgta ccctaacgcg cagcgtatgc agtggggcgt cgtcacgagc gcgggggacg
     4261 cgctggcctg gagcggctcg gcagcgagcc cgtatgcggg gcaccaactg ctcggcgaag
     4321 actcgcgcta cacggccaca tttcaaggca atttactaac gtcggaccga gtgctcattc
     4381 aggcgcgcgc cgcattctcg aatgcgggtt gtgacttggc cgagcggctc atgctcagcc
     4441 tcgaggccgc cggtgttcag ggcgaagggg atcgccgttg tacgcccgac gggcgccccg
     4501 ccaatagcgc atatctcgac gtgacgcttc cggaggggcc gtggcttcac ctgagtgttc
     4561 ccgatgtcgc gcccagcgat ccaatcgtcg tattgcgcgc tctgttcgag caattccgct
     4621 ccaaacatcc atgtccgagc gcttccgaag cgggcgcccc gccgtcgcag gctgacgctg
     4681 cgcctgggcc gatccgcgga cccgaagcga gcacgagaac cactgggccc gggtgctcgg
     4741 tggcaatgcc gacgcgaggg ggtgcagcga cacacagtgc tcgctcgagc caggtatcga
     4801 cgccagccct tctgtgggcg ggctgggggg cacttctctc gctgctcttg cgactcaatg
     4861 tacgcagatg gagaacgaca ctacgtagat ctcgagttga tcgaagcgcg cctcgagctc
     4921 cgatttcaag tgatggatga actcgaagct cgccgcattg tcttcggtgt ctaccgtgag
     4981 ccggaataga cgatcttcgt aacgagtccc ttcgtgtgtc cagacgccct ggatcgagtg
     5041 cgggttgtag gagagggcac cgaatcgatt gagtacgtcc atcagtgtct tgggaacaca
     5101 ctccatgcac tcgtgcatga tggcgcgacc gtcgttgtag cggtcgggca gtagtatttc
     5161 gaagcgcctt cttcgttcga gagccacccg ggtcattgga acattcgcct ttcggttcgc
     5221 gttcgttcag cggcaaatcg tgccgtcgta gcggccttgc ccgagattgc cctgaaaggt
     5281 gcccgatgcg cgccacgcgc cgctcgcgtt cgcgaacacc ccggtcccgc ccacgatcgt
     5341 atccacttcg gcgaagtcgc ccgatccggt tagacgaaac acgatcgcgt ctttggttcg
     5401 tagggttccg ccgcgcaggc tcacttccgc atcgccggtg aacaccagca ccgaggtgct
     5461 gggactatcg atggtttcga gcgaactcgt ggctacgaaa gtggaagtcg cgttcaggtc
     5521 tcctcgccat tcgacgcggc cgcacagccc aaccgccgac gggcaatcgg gacccgcgct
     5581 ggtctcgacc gtgaacgtgc cccgcacggg gcggcacgtg ggccccgaag tgctcgcatc
     5641 atgggtggcg cccatgggat cgggggcggc gtcttcgaga gcggctcccg gcaagggtcg
     5701 agtttgacca gaagtgctga gcgccagggt tccgagcgcg atcagcgaga acgttccgag
     5761 tcgacgaatt ggctggaagc aaacctgttt catgcactcg gctacgtggc agtgctcgcg
     5821 gggttacacg cttggtttgg tgcgcgttcg gttttggttc cgctgttctc gagggtttac
     5881 cgcgcgctcg gcgcgatcgt ccctgattga gccgaaggtt ggcccgtaac acgctgttcg
     5941 agccgaaatc cgacagcttt tcgcgtctgg ctcgcgcgcc ggctcaagct ttggtaaagc
     6001 gagcgccccc ttctagcgcc gcggagatcg gctccgggcg ccggtttcga ggaacgaccc
     6061 aatgccaagt tcgattttct cccaaatccc tcttgctcct gccgacccca tcctcggttt
     6121 gaccgaggct ttccaagccg ataccaaccc caagaaggtc aacctcggcg tcggggtgta
     6181 tcaggatgct caaggcaagg tgccagtgct gcgggtcgtg cgtgaagccg agcgtcgctg
     6241 gtacgagaag gaagagtcca agacctacct gcccatcgac ggcgttcccg cgtacaacaa
     6301 agcggtagcg ggtctgctgt tcggaaacga ttcccctctc atcgcggcgg gccgcgtcgt
     6361 cacggctcaa gcgctcggcg gtacgggggc cttgaaactg ggtgccgatt tcctgcgccg
     6421 cttcttgccg gaggccgagc tttggctgag ccgtccgagc tgggagaatc accgcgccgt
     6481 gttcgagtcc gccggcttca aggtgaacga gtacccgtat tacgacccca agacgaacgg
     6541 tctggacttc gcggcgatgc gcgcgagctt gaatcagctg cccgcacgca gtgtcgtgct
     6601 gctgcacgcc tgttgccaca acccaaccgg tgtcgatctc gagcctgcgc aatgggaaga
     6661 gatcgtgcag atcgtcaaag cgcgcggcct aatgccgttc ctcgatttcg cctaccaagg
     6721 attcgggacc ggcatcgccg aagacgccca agccttgctc gcgttccaga aggcagacat
     6781 tccgttcctg gttgcgagct cgttctcgaa gtcgttctcg atgtaccgtg agcgagtcgg
     6841 ggcgttgtcg atcatcgcga cggacggaga agaggcgcgt cgcgtgcaga gccagctcaa
     6901 gcgcgtgatt cgcaccaatt actcgagccc gagctcacac ggggcccagg tggtcgcgct
     6961 gattttgtcg gatccagagc tgcgcgcggg gtgggagagc gagctcacgg agatgcgaga
     7021 gcggatcttg cggatgcgct cctcgttcgt cgagcagctc tcgagcaagg gcgtgacccg
     7081 cgatttctcg ttcatcaacg accaacgcgg catgttctcc tactcgggca ttagcctgga
     7141 gcacgtgcgg cgcctgcgct cggagttcgg tctttacatc gtcgacagcg ggcgcgtctg
     7201 cgtggcggcg ctgaacgaga agaacctcgg atacgtcacg gacagcatcg cccaagtgct
     7261 cggagcctcg gcatgagcaa agagacccaa gacgcgttgg tcgcgttagg ccagcgctac
     7321 ctttacccaa actaccggca acccgggctg gtcatgcagt ccggcaaggg cgcgctgctt
     7381 tgggatgtgg cgggcaaacg ctacctcgat ttttacggcg gtatcgccgt gagcgccttg
     7441 ggtcacgctc accccgcgct gattgccgcg ttgagcgagc aagccgcgcg tctgatccac
     7501 gtctcgaatt acttctacaa cgagacgaat gtgcggctgg ccgagaagct ttgtaaggcc
     7561 accggcttcg atcgcgtctt cttcgccaac tcgggcacgg aagcgatcga ggcgtgcttg
     7621 aagctcgctc ggcgccactt cttcatgaag ggcgagccgg aacggctgcg cgtgctcgcc
     7681 ttccacaact cctttcacgg gcgcacgctc ggggcgctcg cggcgaccgg gcaggacaag
     7741 tacaaggagg gcttcggaac gctgccgcac acggtgcacg tgccttacgg cgacttgtcg
     7801 gccgtcaagg cggcgatggg ccgtgacgtt gccgcaatct tggtggagcc catccagggt
     7861 gagggcggcg tgagcccggc gccggccgga ttcctgcgcg agttgcggcg tattgcagac
     7921 gagaccggag cgctgctgat cgcggatgaa gtccagaccg gtatcggacg taccgggaaa
     7981 atgctcggga tcgagcacga cgacgtacga cccgacgtgg tcgcgctggc caaagggctc
     8041 ggcggcggcg tgccaatcgg cgcgatgctg tgccgcgagg cgctcaaaga cgcactgccg
     8101 cccggcagcc atggctccac ctttggcggc aacgcgctcg cctcggcggc ggccttggcg
     8161 gtgctagaaa cgattgagcg cgatcagttg ctcgtgcgca ccgctgccgt gggcgaatac
     8221 ctcgcgcaag gcctgcttgc cgtcagtcgc gagttccccg ccttggtcga aacttcgcgt
     8281 gggcgcggct tgttgcaggc cctggtctta cgcggcaacg tcgacgcgcg cgcggtgctt
     8341 cagaagctgt gggacgccgg gctgttgctc accatcgccg ggggtcaagc tttgcgcttc
     8401 tcgccgccgc tgatcgtcag cgacgcggag atcgacgaag cgctggcggt cattcgccga
     8461 gtgttcgcct cgctctcccc gagcgccgcc gagaccggcc ggtgaacgcg gcgcgggcga
     8521 cctcggatcc aagcaagctg ccgtgcgcca cgtgcggcag cgacgtggat ccgttgcgtg
     8581 ccgatcgcgt cgcgctgttc ggcgagcgtt ttcgctattt ttgcagtgtg gcctgtcacg
     8641 agcgctacga gccccgtgtg cttttgaccc ccctacctgc gccgcgcgaa cgcccgagcc
     8701 aggagctccg cgcggtaccc atcgaagcta cggtccacgt cgaaccggcg gagctgcgcg
     8761 aaatccgtcg catcacgcac tccgaggaca gcgagcccga gcctgtagcg tcgtctgcga
     8821 acgcggagct cgagtcactg caggggctgg aggagcgcgg agccgtaccc gggatgggcg
     8881 agggtacggc ttcggcgatc ggagcgttgc tgctggcctt gtcctcgatc ggagggggcc
     8941 tcgcggtagc gctgtcattg gccggaaact cgaacgtggc ggtcacggca cgcctcgtac
     9001 ttaccgcggt ttcggcctgt gcgctggccg ccgaaacctt cctcggggtc cgcgaagaga
     9061 ccgagcagtc gccgctcgcg cggctcgccg cgcccgtggg cgccgtgctg ctcgcgatct
     9121 tcgcgtacgt gagcgccagc gagtctacca acgggatcgt gaccttggcg gggctgttgg
     9181 tcgcgagcag cgcgctgtcg gttttgctcg tccggcgcgt gcgtcgccct gtcgacgtgg
     9241 agcgaacggc gctcggtgcc gaactcgacg gcacgtgcaa gcgcgtggtg ctcgacgagg
     9301 agctggccga aacacgtgcc gtcgatctgc ggcccggtga agaaatcgtg ctcgacagtg
     9361 gcgatgtcgt gccggtcgac gccactttga ccgcgggctc ggccgtcgtg ctgccgtggc
     9421 ggggcgcgag tgttcgcgct gagcgacacg agggagatgt gctcgtcgcg ggtgcgcgcg
     9481 t
//
LOCUS       DASTKU010001039         6264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1098669, whole genome shotgun sequence.
ACCESSION   DASTKU010001039 DASTKU010000000
VERSION     DASTKU010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098669"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VFQ61_36865"
     CDS             <1..1459
                     /locus_tag="VFQ61_36865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960139.1"
                     /translation="TTGFRPRQPTLPMGVAPATLVNRDLTGPEHTPTTTQVTPTAAAV
                     EEAGVPTPAAHANAAAPSSRPSVPRGSVGNTTAVLATPPQPAELRATSTSAAAPTRDT
                     RETVRLTKRKDTPPQAPRVMTVPELSDDEPTVLRPSPFAELRKTPRPMSPEDVVRELE
                     RQGMSDVLDRPPEKTASPAAGLVPEFLASPTAARPADVMSIPTPVAIPVHSVHSQAVH
                     SQTASAHAAAPVPPPALPSTPFAVSAMDASHPVAVSSPLPAPMPAAGTASLRPDEIEA
                     LRTPKNRVMWIFLALMVLVAIAFAALTLTRTENTPKSGGKGTDPAAAENAAAEATPAG
                     VAPAGSESPTGSDGANSPNAASAANGADSAAAPSGAAPSSSGGFAKLFAAGAKQAAGP
                     SDQLQPFDANAARVALDGLMLSAARCREPGGPLGVARVVVTFAPSGNVTSATITDAPF
                     AGTNVGTCLCDVMKRATITPFEGPPGNVTQRVSLR"
     gene            complement(1533..2570)
                     /locus_tag="VFQ61_36870"
     CDS             complement(1533..2570)
                     /locus_tag="VFQ61_36870"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET9960140.1"
                     /translation="MPGLSIIGTGRFLPGRPYTNHDLARVLDTNDAWIQQRTGIRQRH
                     FCPIGQGASHLALPAAREALASARLGPEDIDYILFNTMTPDHVFPGSGPLLGAALGCT
                     GVPALDLRTQCAAMLFAFQTAEALLVSGAARRILIVGAEAHASVMPWRDWDILSGERE
                     GRPSEEDWKRASDERGWSIIFGDGAGAIVVERAERPDAGILAMDLHTDGRYAEQLCTR
                     EGFRVGQAWQSPPFIHMAGRDVFKHAVTKLPRSIEAVCQKAGVPMESIDWFVAHQANQ
                     RINEAVCERLGVTPEKMPSNIDRYGNTSAATIPILLDEMNRDGRLKRGQLVCMLALGA
                     GFHWGSALIRY"
     gene            complement(2701..4005)
                     /gene="aroA"
                     /locus_tag="VFQ61_36875"
     CDS             complement(2701..4005)
                     /gene="aroA"
                     /locus_tag="VFQ61_36875"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET9960141.1"
                     /translation="MAKSAPLSLVPLSKAPDLVWRVPGSKSITNRALILAALSEGVTE
                     LSGVLESDDTRHMRAALTALGIQIEGSGTEWRVWGGRSRLRPAKEPLFVGNSGTTVRF
                     LAALCALVDGETTLYGDAAMSRRPIAVLVDGLRALGVRVECETGCPPLKIHGGRLRGG
                     RVQMQGELSSQYFSALLMAAAGAEADVEVEVLGTLVSRPYVDITLRMMRDFGGSAEAG
                     ADSFRVHARGGYSARNYQVEPDASAASYPFALAAASGGRIEVPGLGTQALQGDYAFVD
                     ILERAGARVERFADRTIVQGPPTLSGVDVDMRDVSDTVMTLAAIAPICRGATRIRNIA
                     NIRLKETDRLAATAAELERLGQAVQTGEDWLFVEPAPLQAALVHTYDDHRMAMSFAIL
                     GAVRSGVQIEDPACVAKTYPRFFEDLGQAYLSVGDRVPWQRG"
     gene            4321..5487
                     /locus_tag="VFQ61_36880"
     CDS             4321..5487
                     /locus_tag="VFQ61_36880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HET9960142.1"
                     /translation="MARIERGARGAFEARLGGKGLVLDPFCGTGTTALACAERGIPAI
                     TTDINPFLIWLAKAKSRRYSLASLERFDVLSRRVQREITVRAGKVSRGAGKARRTAHW
                     RPDLYQIEKWWEAGVLEVLAQAWHLIRVKLADEARPVRDLLELVFCQVMIASAQVSFG
                     HQSMSFRRGKAGSPPALDELFRLSAERISRGATSPIARLPRVLRCDARELATRLEPSS
                     VAQVITSPPYPNRMSYVRELRPYMYWLGYLADGRAAGELDWSAIGGTWGTATSRVGRW
                     EPAAEDRAPSRSLTPALRAIARRSELLSRYVHKYFCDMFRHLEGLGPLLQPNATLHYI
                     VGNSKFYDAVLPVERIFAELFANLGWDDVTVTTLRKRSSKRELFEFEVSARKPA"
     gene            5639..>6264
                     /locus_tag="VFQ61_36885"
     CDS             5639..>6264
                     /locus_tag="VFQ61_36885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HET9960143.1"
                     /translation="MAALGSHPYAKFLDRVSKPTRYTGAEYGVRRKDWQSVQARICLA
                     FPDIYDIGMSHLGFRILYRILNDDPRTLAERAYTPWVDMQKELREHGKLLVSLESARP
                     LCDFDMVGFSLQYELTYTNVLSMLELSGIPLRSCQRSDDDPLIVAGGPVATHAEPMAD
                     FFDAILIGDGEEAASEIALSFAEGKRAGKSRAERLFELSQIRGVYVPSL"
BASE COUNT         1134 a         2054 c         1979 g         1097 t
ORIGIN      
        1 caccacgggg tttaggccgc ggcagccgac gctgccaatg ggtgtcgcgc cagccaccct
       61 agtgaaccgt gacctgacgg gccccgagca taccccgacc accacgcaag ttacgcccac
      121 ggctgcggcg gtagaagaag ccggagtccc cacgcccgct gcacatgcta acgccgcggc
      181 tccgtcgtcg cgcccttcgg taccgcgtgg ttctgtgggt aatacgaccg ccgttctagc
      241 gactccccct cagcccgcgg agctgcgggc gacttctacg tcggccgcag ctcctacgcg
      301 cgacactcgc gagaccgtca gactcaccaa gcgcaaagac acgccgcctc aagccccaag
      361 ggtgatgacg gtgccggagc tgagcgatga cgagcccacg gtcttgcgtc cgtcgccgtt
      421 cgcggagtta cgcaagacgc cgcggccgat gagcccagaa gacgtggtgc gtgagctgga
      481 gcgtcagggc atgtccgatg tgctcgatcg gccccccgaa aagacggcct cgcccgcggc
      541 cggcttggtt ccggagtttc tggcgtcacc tacggcggct cgaccggcgg atgtgatgtc
      601 cattcccacg ccggtggcca tcccggtgca ctctgttcac tcacaagctg ttcactcaca
      661 aactgcttcc gctcacgcgg cggcgccggt gcctccaccc gccttaccga gcactccgtt
      721 cgccgtctcg gcgatggatg cttcccatcc cgtcgcggtc agttcgcctt tgccggctcc
      781 gatgccggcg gcgggtactg catccttgcg gcccgatgag atcgaggcgt tgcgcactcc
      841 gaagaaccgc gtgatgtgga tcttcttggc gctgatggtg ctggtcgcga tcgcgttcgc
      901 cgcgctgacg ctgacgcgca cggagaacac gcccaagtcg ggcggcaaag gaaccgatcc
      961 cgcagccgct gagaacgcgg ccgcggaggc cactcccgct ggtgttgcgc ccgctgggtc
     1021 agaaagccca accgggtcgg atggagccaa cagccccaat gcagcgagtg ctgccaatgg
     1081 tgcggacagc gccgccgctc cgagcggcgc ggcgccgagc tcttccggcg ggttcgccaa
     1141 gttgttcgcg gccggcgcaa agcaggccgc aggtccctcg gaccagctgc agcctttcga
     1201 cgcgaacgcc gcgcgggtcg cgttggacgg tctgatgctg agcgccgcgc gttgtcgaga
     1261 gccgggcgga ccgttgggtg tcgcgcgcgt ggtggtgacg tttgctccca gtggcaacgt
     1321 cacctcggcg accattacgg atgcaccgtt cgctggcacg aacgtgggca cttgcttgtg
     1381 tgacgtcatg aagcgcgcca ccatcacgcc gttcgaaggc ccccccggca acgtgaccca
     1441 gcgcgtaagc ttgcgctgag ctcgaccgag caaggccgcg agcgcgcacc ctttgagccc
     1501 gtgctgggac cgaactcgca ataccgagct cgctagtacc gaatcagcgc gctgccccag
     1561 tgaaacccgg cgccgagcgc gagcatacac acgagctgcc cgcgcttcag tcggccgtca
     1621 cggttcatct cgtcgagcag gatggggatc gtggccgcgg atgtattgcc gtaacgatcg
     1681 atgttgctcg gcattttttc aggggtcacg ccgagccgct cgcacacagc ctcgttgatg
     1741 cgctggttgg cttgatgcgc gacgaaccaa tcgatggatt ccatcggcac cccggccttc
     1801 tggcatacgg cctctatcga ccgcggcagc ttggtgaccg catgcttgaa cacgtctcga
     1861 cccgccatgt gaatgaaggg cggagactgc cacgcttgcc cgacgcggaa tccttcacgg
     1921 gtgcagagct gttccgcata ccgcccgtcc gtgtgcagat ccatggcgag aattccggcg
     1981 tcgggccgct cggcacgctc cacgacgatg gctccggcgc catctccgaa gataattgac
     2041 catccccgct cgtcagacgc tcgcttccag tcttcttcgc tcggccgccc ttcgcgttcg
     2101 ccgctcagga tatcccagtc tcgccagggc atgaccgaag catgagcttc cgcgcctacg
     2161 attaaaattc ttcgcgcggc tccgctcacg agcagagcct cggccgtctg gaaagcaaac
     2221 agcatcgcgg cacactgcgt ccgcaggtcg agagcaggaa cccccgtaca cccgagcgcg
     2281 gcccccaata aaggcccgga gccgggaaac acgtgatccg gcgtcatcgt gttgaacagg
     2341 atgtaatcga tgtcctcggg tcctagccgc gcggaggcca aggcctcgcg cgcagctggc
     2401 aacgccagat gcgaagcgcc ttgaccaatt gggcaaaagt gtcgctggcg aatgcccgtt
     2461 cgctgctgaa tccaggcgtc gttcgtatcg aggacgcgcg ctagatcgtg gttcgtataa
     2521 gggcggccgg gcagaaaacg cccagttccg atgatgctga gacccggcat agaaatcttg
     2581 agctgctacg aagaacgaag tggtgttcct agcctagtcg ccctgggacg tcactcgaag
     2641 acgcgagcca taaggcaaga agcaggtgac ccgacgcaat aagcgggcga aattcttgag
     2701 ctaacctcgt tgccaaggca ctcggtcccc gaccgaaaga taggcctgcc ctagatcctc
     2761 gaagaaccgc ggataggtct tggcgacgca ggccggatcc tcgatctgca cgccactacg
     2821 cactgcgccg agaatggcga agctcatcgc cattcggtgg tcgtcgtagg tgtgcaccaa
     2881 agcagcttgc agcggagccg gctcgacgaa cagccagtcc tcgccagtct gcacggcttg
     2941 gccgagccgc tctagctcgg ccgcggttgc cgcgagccga tcggtctctt tgagccgaat
     3001 gttcgcgatg ttgcggatcc gcgtcgcacc gcggcaaatc ggggcgatgg cggccagagt
     3061 catcacggta tcggagacat cgcgcatatc tacgtccacg cccgacaacg tcggggggcc
     3121 ctgcacgatc gtgcggtccg caaatcgctc tacgcgggcc ccggctcgct ccaggatgtc
     3181 cacgaacgcg tagtccccct gcaaagcctg agtcccgagg ccaggaactt cgatccgccc
     3241 cccgctggcg gccgcgagcg cgaacggata actcgcggca gaagcgtcgg gttcgacctg
     3301 gtagttgcgg gcggagtatc cgccccgcgc gtggactcga aagctatccg ctcccgcttc
     3361 cgcggagccg ccgaagtccc gcatcatgcg cagggtgata tcgacatagg ggcggctcac
     3421 gagggtaccc aatacttcga cttcgacgtc cgcctctgcc cccgccgccg ccatcaacag
     3481 cgccgagaag tattggctcg aaagctcacc ctgcatttgc acgcgcccgc cacgcaggcg
     3541 gccaccatga atcttcaaag gtggacagcc ggtctcgcat tccacgcgga caccgagggc
     3601 gcgcagacca tcgacgagca cggcgatggg tcggcgactc atggcggcgt cgccgtaaag
     3661 agtggtctca ccatccacca gcgcgcagag agccgcgagg aagcgcacgg tcgttccgct
     3721 gttgccgacg aacagcggct ccttcgctgg gcgcagacga gaacgacctc cccagacgcg
     3781 ccactcggtt ccactcccct cgatttggat tccgagcgcc gtcagagcag cgcgcatgtg
     3841 tctcgtatca tcgctctcga gcacaccgga tagctccgtg acgccctctg atagcgccgc
     3901 cagaatcaag gctcgattcg tgatgctctt cgagccgggc acacgccaaa ctagatccgg
     3961 agctttggag agcggaacga gggaaagggg agccgacttg gccatgcggg aaccttgggg
     4021 ggtggatctt ggggtccgag cgtgtagcac tttactcgct gactacgagt gcataaaaaa
     4081 ctgctgcctt tcgagtgcgc gccgataggg cgccgtgata gccgatctgg ccgcgaggac
     4141 ccgctgagac ccgctaccca ggatccccat ccacccgaag cgcgagcgac gagcggctcg
     4201 cccgcgcccc gtcgcgatca tctgacgttt cgaggcaacg aacctaaccg tcggtatggc
     4261 tggctacgac tcacgcccgc ctactcgctg cacgtcgtcg aaaggctcct cgacgagagg
     4321 gtggcgagga tcgagcgagg agcacgcggc gcgttcgaag cacggcttgg tggcaagggg
     4381 ctagtgctcg acccattctg tggtaccggc acgaccgcac tcgcgtgcgc ggagcgtggc
     4441 atcccggcaa tcaccacgga catcaatccg ttcctgattt ggctggcaaa ggccaagtcc
     4501 cggcgttaca gcttggctag cctcgagcgc ttcgatgttc tctcgcgacg cgtacagcgc
     4561 gagatcactg tacgagcggg caaggtctcc cgcggagcag gaaaggcccg ccgcaccgcg
     4621 cactggcggc cggatttgta ccaaatcgaa aagtggtggg aagcgggtgt gctcgaggtt
     4681 cttgcgcaag cttggcatct gatccgggtg aagctcgccg atgaggcgcg cccggtccga
     4741 gacctgctag agctcgtgtt ttgccaggtg atgatcgcat ccgctcaagt cagcttcggg
     4801 caccagtcga tgtctttccg acgcggcaaa gcgggctctc cccccgcgtt ggacgagctg
     4861 ttccggctgt cggcggagcg tatctcgcgt ggggccactt cgcccatcgc gcgcctcccc
     4921 cgggttttga ggtgtgatgc gcgtgagctt gcgacgcgcc tcgagccgag cagcgtggcc
     4981 caagtcatca cgtccccgcc gtatccaaac cgcatgagct atgtgcggga gttgcggccg
     5041 tacatgtatt ggctcggata cttggcggat gggcgtgccg ccggggaact cgactggagc
     5101 gcgattggag gcacttgggg aaccgcgacg agccgagttg ggcgatggga gcctgccgcg
     5161 gaggatcgcg ccccgagccg tagtctcact ccggccttgc gcgcgattgc ccgccgcagc
     5221 gagctgctct cgcgctacgt acacaagtac ttctgcgaca tgttccggca cctggagggc
     5281 ttggggccgc tgctccaacc gaatgccacg ctccattaca tcgtggggaa ctccaagttt
     5341 tatgacgcgg tattgccggt cgagcgcatc tttgcagaac ttttcgcgaa cttgggctgg
     5401 gatgacgtca cggtgacgac actgcgcaaa cgatcctcca agcgcgaatt gttcgaattc
     5461 gaagtatcgg ctcgcaagcc agcataagcc ttggcccgct gaaaatccga gggaaggccg
     5521 cttcgccgag acggagttcg agtgggcgag cccgtaaggc cgcgcgggga gagcgcgctc
     5581 gcgcctcacc ttttgcaact agcgaagcgc cgaactcaag gtcagagtag ccgaccccat
     5641 ggcagccctc ggttcgcatc cctatgcgaa gtttctcgat cgcgtcagca agcccacccg
     5701 ctatacgggc gccgagtacg gagtgcgtcg taaagactgg cagagcgtac aggcgcgcat
     5761 ctgcctagcg ttcccggaca tctacgatat cgggatgagc catctcggct ttcggatctt
     5821 gtaccggatt ctgaatgatg atccgcgcac gctcgccgag cgcgcttata cgccctgggt
     5881 cgacatgcag aaggagctgc gcgagcacgg caagctgctg gtttcgctcg agagcgcccg
     5941 tccgctgtgc gacttcgaca tggtgggctt ctcgctgcag tacgagctca cctatacgaa
     6001 cgtgctgagc atgctcgagt tatcgggcat tccgctgcgg agctgtcagc gcagtgacga
     6061 cgatccactc atcgtcgcgg gtgggcccgt cgccacccac gccgagccga tggctgattt
     6121 cttcgacgcg atcctgattg gcgatggcga agaagccgcg agtgagattg cgctcagctt
     6181 cgccgaaggc aagcgagcag ggaaatcacg cgccgagcgg ctgttcgagc tctcgcaaat
     6241 ccgtggcgtg tatgtgccct cgct
//
LOCUS       DASTKU010001040        10372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_206271, whole genome shotgun sequence.
ACCESSION   DASTKU010001040 DASTKU010000000
VERSION     DASTKU010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206271"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFQ61_36890"
     CDS             <1..390
                     /locus_tag="VFQ61_36890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9960144.1"
                     /translation="LYVDTHVPGSSRPQPEDLRQRFVEAVEARIEGLAPGFRHGILER
                     RVTAPNDFMRGNRNLVDGDLGGGSSVWSQQLCFRPFFRGFRYRMPVGRVYLCSSSTHP
                     GGGAHGMCGYNAALRVIGDLASGHLAA"
     gene            459..2792
                     /locus_tag="VFQ61_36895"
     CDS             459..2792
                     /locus_tag="VFQ61_36895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960145.1"
                     /translation="MSEPSPARPKPASTAPRPLRSAWISGCALALCVALSFTGYAFLA
                     PATYRASATVVLTAEGADRHALGPLEASRRLSEAILDKDLLEQLTREIAPGQDGGARL
                     RAGRALRDALEVESVDGLRFTISSRAPSSERARVLTQRFAEKAAAVAPRVLAADRRAE
                     RQRLEAERRRKSDELVGFLTQHPELKSEPTTVGASEATASATRAQALRVLLAERANVE
                     ARLATRGMTPPSDNPYAEADTNDAEADQLERRLSQLNTAIFAERNAARSRAAPPSAPA
                     KAGTAQNWAEFQRLLREVEEARNASEALRNARPAVHASVSAPAVLPTQPIEPNRKALL
                     GWCAWLAPLSGALLGAFVFVRSNRRLRTLLWSSAPPPSDLRSEPPPPPDLPKNLDLPN
                     LGPPNANAASFIELSSAAGGASTQSNPFAKPPTASAPSPWFDQDAKLASALPNTAPGA
                     KGRASQPPPQTVPTRERRSSRPPAAGYSSQPPVAAPLGANAGAPGGAGVMRTTQKLGS
                     PIPPILVPGRGSNSSIPPGPASEGGYRFVSSPPPVSPNAVRITRDSTPILALPVNPTW
                     QPDAHLNPDSRKALCDEIYPLAVDHCFVVGVSGTSVTRAEKSRLAAELALALSESEHP
                     RVLLLEGDLMSPAIHTLMQVEMPKGRGLTEQLEERVAHSSVRAWVVMECLRSLHLLGE
                     GPRPKTTLMLSHYFERCVQELRSYYDFILINGPILSEIAYCRALHDVVDEVLLVHATP
                     NAADVAEGFELFNGKRLGLVQAPIDATAEPASQAQPR"
     gene            complement(2869..4710)
                     /locus_tag="VFQ61_36900"
     CDS             complement(2869..4710)
                     /locus_tag="VFQ61_36900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HET9960146.1"
                     /translation="MLVQRSDAPGDSGERMRVPAFYKRRRKRDNEIGQVLLRKGFITP
                     EQLRQALRTQQESGGHVGAILRRMGACDSRAIAEALIEQVRLHREKGKSKNLARNARE
                     NPSIIGLDVRCKPWTVVACILASDVLLFAIAAALVWLLGSPELLTRMQRWGVVSLVPL
                     NVGAFAALQLYTVTPQSPPEEIRTATAATTLIYLGSWLLTALTLVGPFAALDHAAWIM
                     GGLFAAVAVPIGRGIVRSKFSKRPWWGYPVVVMGAGRVGRAVVSTLQSRPQLGLKPVA
                     ILDDDPEKQGTLRVSWGEDDIVFERVRGNDELHQDVETPSTRFALEQFAEVEGVPVVG
                     GFELAPMMAQRLGIRTAVIAMPEMDAAGVLSIIERHADGYINVLVIPDLFNLAHFGAP
                     TKYLGGVLGIEVQRQLLLRWPRIAKRALDLVLTMIGGVLILPILATLALLVKLDSKGP
                     IFYPQRRLGQDGVRFAALKFRTMYQDAERRLQELLARDPKLRAEYEEFHKLAADPRVT
                     RIGRFLRRYSLDELPQLWNVIKGDMSLVGPRPYLEREIPEMDQKEAIILRVKPGITGI
                     WQVTWRNESTFEQRVQLDVEYVRNWSPWLDLYVLARTVPVVLGGTGS"
     gene            complement(5098..5337)
                     /locus_tag="VFQ61_36905"
     CDS             complement(5098..5337)
                     /locus_tag="VFQ61_36905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960147.1"
                     /translation="MQSVKGMVQVAGTTYRIVRVGTGVYDVVRILDDARVGSFRSFPR
                     VEIGSSSIDQDLMREIARAAIQGAKTSWVGRFTLP"
     gene            5452..5868
                     /locus_tag="VFQ61_36910"
     CDS             5452..5868
                     /locus_tag="VFQ61_36910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960148.1"
                     /translation="MLFCVSGFLRWWFSRAFCAGGSSALSALVVLPRLLRWWFSRASG
                     WDALFGWHALLARLLIAWGGGLLRWFLALVSGAGFWRWFWRGGFWRWFLARRFLARRH
                     ERFYRRVSAVFSTKLDPVRYAVRLEDAMRLLLALQQ"
     gene            complement(7795..>10372)
                     /gene="uvrA"
                     /locus_tag="VFQ61_36915"
     CDS             complement(7795..>10372)
                     /gene="uvrA"
                     /locus_tag="VFQ61_36915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9960149.1"
                     /translation="LDAEAGRRVMVMAPLERQRKGVHRDRLESLASQGFARIRIDGKV
                     TPINEAFELLDKKKKHDLDLVIDRIVVDEAQRARLGDSVETALREGGGSVLFTGDDFE
                     RVFSERAACVKCGISFGELTPLSFSFNSPLGFCTECNGLGSRPEMDPDLLVPNPALSI
                     RNGAVAPWAAAMSRGDGWINNEVTWIADTFGVDLDRPWEKLPKAQRDVVMYGSKRGRT
                     SWEGLVNQLMRRLKATTSEDMKQYYLRYFSSKPCPACSGARLRPESRAVKIHGVSIDR
                     VSAMRIEEALRWIDAMPLSRVEARIAEELRKEIRSRLGFLANVGLGYLSLDRGAASLS
                     GGESQRIRLASQIGSELTGVIYVLDEPSIGLHQRDNRRLLETLKALRDLGNSVLVVEH
                     DEETMLDADYIVDFGPGAGELGGEIVAQGTLEEVKASERSITGAFLSGRRRIELPAER
                     RPGNGHFLEVRGARENNLANLDVRFPLGTLIAVTGVSGAGKSTLINGILRPALSRALH
                     ESRDAAGEYGALLGVEHVDKVIAIDQKPIGRTPRSNPATYTKVFDQIRDLYASLPEAR
                     AFGYDAGRFSFNVKGGRCEACSGDGVRCVEMHFLPDVYVTCEVCKGRRFNDATLRVRF
                     KGLSIADTLDLSVRHALEVFSVHTGILRGLQTLDDVGLGYIKVGQPSPTLSGGEAQRI
                     KLSRELSRVGTGRTVYVLDEPTTGLHFADVERLLHVLSRLVDAGNTLIVIEHNLDVIA
                     HADWVIDLGPEGGSGGGRVVAEGTPERVAGVSESYTGRYLAELLRGRGRAISVAASAA
                     PSTSSTTGPSSAVSSRRRGTFGDASSTLQPRTRTKRGNISAPVGQIADGNRRGRKASL
                     KV"
BASE COUNT         1854 a         3339 c         3108 g         2071 t
ORIGIN      
        1 ctttacgtag acactcacgt gcccggcagt tcccgacctc agccagaaga cctaaggcag
       61 cgctttgtcg aagccgtcga agcacgcatc gaagggcttg ctccgggttt tcgccacggc
      121 atcttggagc gtcgggtgac cgcgcccaat gatttcatgc gcggcaaccg gaacttggtc
      181 gatggagacc tgggcggagg ctcgagcgtc tggagccaac aactctgctt tcgaccgttt
      241 tttcgcggct ttcgataccg gatgcccgta ggtcgagtct acttgtgttc gagctcgacc
      301 cacccaggcg gcggggcaca cgggatgtgc ggctacaacg cggcgctgcg ggtgatcggc
      361 gacttggcat ccggccacct ggccgcgtag atcgctcgca cttggcgtcg ctcaagaacg
      421 cgattttcct gtagattgag cagtgctcgg ctggtccgat gtcagagccc tcccccgcgc
      481 gcccaaagcc ggcgtcgacc gcgccgcgac cgcttcgttc cgcctggatt agtggctgcg
      541 cgctagcgct ttgtgtagcg ctgagcttca cgggatacgc tttcttggcg ccagcaacct
      601 accgcgcgag cgccacggtg gtgctgaccg cggaaggagc ggatcgacac gcgctcggtc
      661 cactcgaagc atcgcgtcgc ctgagcgaag ccatcctgga caaagatcta ctcgagcagt
      721 tgacgagaga gatcgcgccg ggacaggacg gcggtgcgcg actgcgcgcg ggccgagcgc
      781 tgcgtgatgc actcgaggtc gagtccgtcg atgggctgcg cttcacgatt tcttcgcgag
      841 ccccctcgag tgagcgtgcg cgcgtgctta cccaacgttt tgcggagaag gccgcggccg
      901 ttgccccgcg cgtcttggcg gcggaccgtc gcgcggagcg ccaacggctg gaagccgagc
      961 gccgtcgtaa gagcgacgaa ctggttggct ttctgacgca acacccagag ctgaagtccg
     1021 agcccaccac cgtgggagcc tcagaagcta cggccagcgc cacccgagcc caagccttgc
     1081 gcgtattgct cgccgagcgt gcgaacgtcg aagcgcgcct cgcgactcgc ggtatgacgc
     1141 cgccctcgga caatccttac gcggaagcgg acaccaacga cgcggaggca gatcaactcg
     1201 agcggcgcct gtcgcagctg aacacggcga ttttcgcgga acgcaacgcg gcgcgcagcc
     1261 gcgccgcccc accgagtgca cccgccaagg ccggtacggc gcaaaactgg gcagaatttc
     1321 agcgtctgct gcgcgaggtc gaggaagcgc gtaacgccag tgaagcgctg cgcaatgcgc
     1381 ggcccgcggt tcacgccagc gtgagcgcgc ccgcagtctt gcccacgcaa cccatcgagc
     1441 ccaaccgcaa ggcactgctc gggtggtgcg cctggctcgc gccgctctcg ggggcattgc
     1501 ttggcgcgtt cgtattcgtg cgcagcaacc gaaggctgcg cacgctcctg tggtcgagcg
     1561 caccaccgcc cagcgatctt cgttccgagc cgccgcctcc cccagattta cccaagaacc
     1621 tcgatcttcc aaatctcggt ccgcccaatg caaacgccgc cagcttcatc gagctgagct
     1681 ccgcggccgg cggagcgtcg acccaatcca atccgttcgc gaagcccccg acggcaagtg
     1741 ccccgtcccc gtggttcgat caggacgcaa agcttgcatc tgctctcccg aacacggcgc
     1801 cgggtgccaa agggcgggct tcgcagcctc cgccacaaac ggtccccact cgcgagagaa
     1861 gatcgagcag gccgcctgcg gctggttact cgagccaacc accggtcgcc gcgccgctcg
     1921 gagcgaacgc cggcgcgcca ggcggcgcgg gcgtgatgcg cacgactcag aagctcggct
     1981 ctcccattcc acccattctg gtcccagggc gcggttcgaa ttcgagcatt ccgccggggc
     2041 cggcatcgga aggcggctat cgcttcgtca gctcgccacc gcccgtttcg cccaacgcgg
     2101 tgcgtatcac gcgggattcc acgcccattc tcgcgctgcc cgtaaatccc acgtggcagc
     2161 cggacgcgca tttgaacccg gattcacgca aggcgttgtg cgacgagatt tacccgttgg
     2221 ccgtggatca ctgcttcgtc gtaggcgttt ctggcacgag cgtgacgcga gcggagaaat
     2281 cgcggctagc cgccgagctc gcattggcgc tctcggagag cgagcatccc cgcgtgctgc
     2341 tgctcgaagg ggatctgatg agccccgcga tccacacgct catgcaagtc gaaatgccga
     2401 agggacgcgg cttgacggaa cagctcgagg agcgagtcgc gcactcgagc gtgcgtgctt
     2461 gggtagtgat ggagtgcctg agatctctgc atctgctcgg agaaggcccc cgccccaaaa
     2521 ctacgttgat gctctcgcac tacttcgaac gctgcgttca agagctgcga agctactacg
     2581 acttcattct gatcaacggc ccgatcctga gcgagattgc ttattgccgg gccttgcacg
     2641 acgtggtgga cgaagtgctg ctggttcacg cgacgccgaa cgccgccgac gtggccgaag
     2701 gcttcgagct gttcaacggg aagcgcctcg ggctcgtaca ggctccgatc gatgccaccg
     2761 cggagcccgc ttctcaggcc cagcccaggt aagccgcggg ggggtgcctc ggcgaatccc
     2821 gatccgaagg aatgagcgcg ctccgagttt cgagcgcgct cacttgcttc aagagccggt
     2881 gccgcccaag accaccggga ccgtgcgagc gagcacgtac agatcgagcc acggcgacca
     2941 attgcgcacg tattcgacgt ccaactgaac acgctgttcg aaggtcgact cattacgcca
     3001 ggtgacttgc caaatccccg taatgcccgg cttgacccgc aggataatgg cctccttctg
     3061 gtccatctcc gggatctccc gctccaggta agggcgaggg ccgactagac tcatgtcgcc
     3121 cttgattacg ttccacagct gcggcaactc atccaagctg taacgccgta agaagcgacc
     3181 gatccgcgtc acacggggat cggcggcgag cttgtggaac tcctcgtact cggcgcgcag
     3241 cttgggatca cgcgccaaga gctcctgcag gcgccgctcg gcatcctgat acatcgtgcg
     3301 gaacttcaat gccgcgaatc gcacgccgtc ttggccaagc cgacgctgtg ggtagaagat
     3361 tggccctttg gagtccaact tcacgagcaa ggccaaagtc gccaagatgg gcaggatcag
     3421 aacaccaccg atcatcgtga gcacgagatc gagtgcccgc ttggcaattc ggggccaacg
     3481 gagcaacagc tgacgctgca cttcgatgcc gagcacgccg cccaggtatt tcgttggcgc
     3541 cccgaagtga gccagattga acagatcggg aatcacgagc acgttgatgt aaccgtccgc
     3601 gtgacgctcg atgatgctga gcacacccgc cgcgtccatt tccggcatcg cgatcacggc
     3661 cgtccgaata cccaggcgct gagccatcat cggcgcgagt tcgaacccgc cgaccacggg
     3721 cacgccttcg acttcggcaa actgctcgag ggcgaagcgc gtagaaggag tctccacgtc
     3781 ctggtgaagt tcgtcgttcc cgcgcacccg ctcgaacacg atgtcgtctt caccccaaga
     3841 aacgcgcaga gtgccctgct tctcgggatc gtcgtcgaga atcgcgaccg gcttgagccc
     3901 gagctgcggc cgactttgga gtgtgctcac gacggcccgc cccactcgac ccgcacccat
     3961 cacgaccact gggtatcccc accacggccg cttcgagaac ttggagcgca cgatgccgcg
     4021 tccaatagga accgcgacgg ccgcgaacag gccgcccata atccaggccg cgtgatcgag
     4081 agctgcgaag ggcccaacca acgtgagcgc ggtgagcaac cacgagccca ggtaaatgag
     4141 tgtggtagct gcggttgcag tgcggatctc ttcgggcgga ctctgcgggg taaccgtgta
     4201 gagctggagt gcggcgaacg ctcccacgtt gagcggaacc agggacacga cgccccaacg
     4261 ctgcatccgc gtcagcagct cgggggagcc gagcaaccat accagcgccg cggcgatcgc
     4321 gaagagtagg acgtcgctcg cgaggatgca cgcgaccacc gtccagggct tacaacgcac
     4381 gtccaacccg atgatcgacg ggttctcacg cgcattgcgg gccaggttct tggacttgcc
     4441 cttctcgcgg tgcagacgca cctgctcgat gagggcctcg gcaatcgcgc gcgaatcaca
     4501 cgcgcccatg cgacgcagga tagcgcccac atggccaccg ctctcttgct gggtgcgcag
     4561 cgcttggcga agttgctccg gcgtaatgaa gcctttgcgg agcagaactt gccctatctc
     4621 gttatctcgc ttgcgccttc gcttgtagaa cgccggaact cgcattcgct cgccggaatc
     4681 acccggggcg tcggaccgct ggacgagcac gggatcacct tccggttaga aaatcttcta
     4741 ccaagatctg gaggtcagag ggagaaatag gtagtgggaa cgatctcaaa ccgcaaactc
     4801 tgaggcctcc caggccgaaa agcaacgggc tccgcaactg gagcgccttc cgaagcactt
     4861 tccggccctt cgcgcagttc tgtgcccgtt tggttggggg aaggtcgctc ttggaaggtg
     4921 cggctgtcgt tgttccgggg atgcgaggtg agctcgctgg ctcggcgctc tttgggtcgg
     4981 ctggcgctcg ctgggctggc gctctttggg tcggctggcg ctcgttgggc tgcgttgtca
     5041 gggccggagc tctggttgcg gccgggtcag tgccgttctc tgctcggtgg catcggttca
     5101 gggcagcgtg aagcggccaa cccagctcgt tttggcacct tgaattgccg cccgggcgat
     5161 ctctcgcatc aggtcttggt caatgctgct gctcccgatc tccacgcggg gaaaactgcg
     5221 aaagctcccg acgcgtgcgt catcgaggat tcggaccacg tcatagacgc cggttccgac
     5281 acggacgatg cggtaagtgg tcccagcaac ctgaaccata cctttgacgc tctgcatttt
     5341 tttgcccttc agtccgaaag aaactcatac cgtggtttgt gtctcgagag aaggagaaac
     5401 gcttacgagc caaacgtgag ctgtccctgc tgatctgctt tctccgagct aatgcttttc
     5461 tgcgttagtg gttttctgcg ctggtggttc tcccgcgctt tctgcgctgg tggttcttcc
     5521 gcgctttctg cgctggtggt tctcccgcgc cttctgcgct ggtggttctc ccgcgcttct
     5581 ggttgggacg ctctattcgg ttggcacgca ttgttggcac gcctcctaat tgcttggggc
     5641 ggcggcttgt tgcgctggtt tctggcgctg gtttctggcg ctggtttctg gcgctggttt
     5701 tggcgcggcg gtttctggcg ctggtttttg gcgcggcggt ttcttgcgcg gcgccacgaa
     5761 cgtttctatc gccgcgtcag cgccgttttc tccacgaaac tcgatccggt ccgttatgcc
     5821 gttcgccttg aggatgcgat gcgcttgctc ttggctttgc agcagtaggc ggcccagttc
     5881 attttcacga atcggcggca tcgcgtagta gttcgtttga gtgtaccgga gcgccgagtt
     5941 ttcttaggcg gttggctccc cagagtcttt ctcgaggtgg cgaattacgg gtgttcggca
     6001 ggcttgcttg gctatcccac tgcttggcta tcccactgct tggctatcct actgggtgtc
     6061 ccactagact tgcctctggg ccccgcgcgc gtggcagctc cgagcaacct tggaacgttc
     6121 tgaccaagag cggacgcttc gcgcgggagt ttcgccgtga cgatgattct tgtgtcactt
     6181 cgccatctgt tatgcgcttc gcagccgcgt cgggctttga atctcggcga gagctctgtg
     6241 aacgaacctg tgaacgaacc tgtgacccaa tgtgtgaact actctgagaa cgccgaccat
     6301 tcatgagctt gcgtccttgg cggtcgggcc tgtcgcgctt gcccgctcgc actcccgagg
     6361 tttcttcctc tgttgggggt tatctctgtt ggtgttattg cggcgctttc tcaccgcacc
     6421 gttttcctct cgaatttttc ctctcgaatt tcctctcgaa tccctctcac ttcttttcaa
     6481 gagacgattc tcatatctcc gtgaggcggt cgcatcggac ctacctgggc cgtcctttga
     6541 gccctaattc aagtcgtgaa gtcgccctgc tacgactgga agtcaccgct gcactaagca
     6601 cgatctaggc ctgtcactta ctgggattgc tgcatagctg tactggtcga aactcaggcc
     6661 ggtccacgac gtgcaatctt cggacgcgag ccgcggccag cttgaggcgg ttttggtgct
     6721 ctcgtgtaga gctttccgac gtgggttctg ttagcgaaaa ccctgaacgt acgaatttcg
     6781 atcgttcgag ggaactcgat ccaaagggtt ccttaacgca tcggccgggc tgtctcgaag
     6841 gtgtcactgg cacatgagac tttctcgagg tgggcttgcc tacgcgatct tgacctcatt
     6901 cggtgacaga tagcgtcgga ggcccaatcg tttccagagc gctacgcggg aaattggagt
     6961 gtaccgctcg tactggcctg cgcgtgccgg ccggtctgaa tgcacgatac gctgccggac
     7021 aatgggtggt ctaagaaggc tcggttccat gaaatgatca catgtctctc gcgtaattgt
     7081 ctgccccccg cgtttggcgg gatgcctccg gcacgtcgcg ggagccatgc atagcgaacg
     7141 ggtcatgaac gacttagtgg cttgccgaga ttggttacct cagctcgaaa tcagctcgaa
     7201 cggatcagag ccgtaatgga gtgaccaagc ccgttgtctg atgtgcgaag actcgcatcc
     7261 actgcgtgcc agaatcgaca ccagtgcgcc tgccttccca ttgcgctctc cccggcgctg
     7321 tgccctcttg ccattccact ttgcaagcct gcatcgaaaa ggtgccatca cttgaggtgc
     7381 actagcgccg tgtttcccct gatttgttcc cctgagtaag tccgccgcgt tccttttgta
     7441 cacacttgtg gaaccggttt ctgctcgcct ttacccgctc taccgctcca acgtgggtgc
     7501 acgcccgtat tttgcctcct tcgcggatgg gcgttgggac acgtaaggac caggccggtg
     7561 ctttaggacc tggcccggtg cttccgttca tcgcaccggc caaccgccac cctacactta
     7621 cttaatgccc caaccgctca ccaatggatc agtattcctc accgggctga ctaaacgcat
     7681 ccaggaggcg accctccgat ccgaagaagg ccgtccggcg actgcgtcgc agtctccgtt
     7741 accaaaccaa tgatgacgat atgccccagc cgggggtctt tcaatttcag aggcttacac
     7801 tttcaatgag gccttgcgcc cacgtcggtt tccgtcagca atctgcccca ccggtgcgga
     7861 aatgttgccc cgctttgtac gcgttctggg ctgcaaagta gagctggcat caccgaaggt
     7921 gccgcgtcgc ctggaagaaa ctgctgaact tgggcccgta gtcgaggagg ttgagggggc
     7981 cgctgaagcc gccactgaga tcgcccgccc gcgtccgcgc aacagttcgg ccaagtaacg
     8041 tccggtataa ctctccgaaa cgcccgcgac tcgctccggt gtgccttccg ccactacccg
     8101 gccgccgcct gagcctccct cgggtcccaa atcaatcacc cagtctgcat gggcgatcac
     8161 gtcgaggttg tgctcgatga cgatgagcgt attgccggcg tcgaccagcc tggaaagtac
     8221 gtggagcagg cgttcgacgt cggcgaaatg aaggcccgtg gtgggctcgt ccaaaacgta
     8281 cacggtgcgt cccgttccga cgcgtgagag ctcgcgcgac agtttgatgc gctgagcctc
     8341 tccccccgac aacgtcggcg acggttggcc gaccttgatg tagccgagcc ccacatcatc
     8401 gagcgtttgg agcccacgca ggatccccgt gtgcaccgaa aagacctcca gcgcatggcg
     8461 aacgctcaag tccagtgtgt ccgcgatgct caagcccttg aagcgcaccc gcagggttgc
     8521 gtcgttgaag cgacggccct tgcacacctc gcaagtcacg tacacgtcgg gcaagaagtg
     8581 catctcgaca caacgcacgc catcccctga gcacgcttcg caacgtccgc ctttgacgtt
     8641 gaaggaaaag cgcccggcgt cgtaaccgaa agctcgggcc tccggcaagc tcgcgtacag
     8701 atcgcggatt tgatcgaaga ctttcgtgta agtggccggg ttcgaacggg gtgttcttcc
     8761 gatcggcttc tgatcgatgg cgattacctt gtctacgtgt tctacgccca atagcgcgcc
     8821 gtattcgccc gcggcgtcgc gtgattcgtg cagagcgcgg gacaacgcgg gccgcagaat
     8881 cccgttgatg agcgtcgatt ttccggcgcc actcacgccc gtcacggcga tcaatgtgcc
     8941 gagcggaaag cgtacgtcga gattggccaa gttgttctca cgcgcccccc gaacctcgag
     9001 gaagtgccca ttgccgggcc tacgctcggc gggcaactcg atgcgccttc ttccggagag
     9061 gaacgcgccc gtgatggagc gctcgctcgc tttcacctct tccaacgtgc cctgagcaac
     9121 gatctcgcca cccagctcgc ccgcgcccgg accgaaatcg acgatgtaat ccgcatccaa
     9181 catcgtctct tcgtcgtgct ccacgaccag cacggagttt cccaaatctc tcagggcttt
     9241 gagggtctcg agcaggcggc ggttgtctcg ttgatggagc ccaatcgaag gttcgtcgag
     9301 cacgtagatc acgcccgtca actcggagcc aatctgtgac gcgagccgaa tgcgctggct
     9361 ctcacctccg gacaacgacg ccgccccacg atccaagctc aagtatccga ggcccacatt
     9421 ggcgaggaag ccgaggcgcg agcggatctc cttgcgcaac tcttcggcaa tgcgggcttc
     9481 gacgcgcgac aaaggcatcg cgtcgatcca tcgtagggcc tcttcgatgc gcatcgccga
     9541 aacccgatcg atgctcacgc cgtggatctt gactgctcgg ctttcggggc gaaggcgagc
     9601 gccactgcac gcggggcatg gtttgctcga aaagtagcgc agatagtact gcttcatgtc
     9661 ctcgctggtg gtcgccttca agcggcgcat cagctggttc acgagcccct cccagctcgt
     9721 acggccacgc ttactgccgt acatcacgac gtcacgttgc gctttcggaa gcttctccca
     9781 aggccgatct agatctaccc cgaaggtgtc ggcgatccag gtcacctcgt tgttgatcca
     9841 accatcccca cgactcatgg ccgcggccca gggggccacc gcgccattgc gaatcgacag
     9901 cgccgggttc gggacgagca gatcaggatc catctccggg cgcgaaccga gcccgttgca
     9961 ctcggtgcaa aacccgagcg gcgaattgaa agagaagctc agaggagtga gctctccgaa
    10021 cgagatccca cactttacgc aggcagcgcg ctcgctgaac acgcgttcga aatcgtcccc
    10081 agtgaagagc acgctcccgc cgccctctcg cagagccgtc tccaccgagt cccccaaacg
    10141 cgcacgttgg gcttcgtcca ccacgatgcg gtcgatgact agatcgagat cgtgtttctt
    10201 ctttttgtcg agcagctcga aggcttcgtt gatgggagtg actttgccgt cgatgcgaat
    10261 ccttgcgaag ccttgcgacg cgagggactc caggcgatct cggtggacgc ccttgcgttg
    10321 ccgctcgagc ggggccatca ccattacgcg acgccccgcc tccgcatcca gc
//
LOCUS       DASTKU010001041         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1547047, whole genome shotgun sequence.
ACCESSION   DASTKU010001041 DASTKU010000000
VERSION     DASTKU010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547047"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VFQ61_36920"
     CDS             complement(<1..1239)
                     /locus_tag="VFQ61_36920"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9960150.1"
                     /translation="MESELRPSCVETKQTEELRSYRPRGPWLLEVGSPDGGHARVLAA
                     GDRLVVGSGRNADLRVADSTVSQRHCSIRVTEEHVELEDLGSKNGVYVGSVRVARACL
                     REAAAFVVGRTSVALRAGADESEVNAEPVPGLVGSSLGIRKVWSEVRRHATTRAPVLL
                     QGESGTGKDVVARAIHQLSGRSGAYVPLNVGAVPESLADAELFGARRGAFTGAFQSRV
                     GAFEQAHQGTLFLDEIADLPAALQVKLLRVVEDGCVRALGATQVSRVDVRLISASWAC
                     LEERVEASRFRADLFHRLAMVVVVIPPLRQRKADIAELASVWLDRMRSEVGDKRLTSA
                     ALARLLEHDWPGNIRELGGVLYRAAMQADTGYILPEHLGLSGKRDVARVGRLDLGQAQ
                     ELLKSNQGNVSAAARAAGVPR"
     gene            1313..1987
                     /locus_tag="VFQ61_36925"
     CDS             1313..1987
                     /locus_tag="VFQ61_36925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960151.1"
                     /translation="MNARTLLIAARLSVPIGLTSSLAWAQGAPPTPPRESDESHLAPI
                     PRAKDLLSGHVLVGASGRFVVPFGEFDDSTDVRDIARPSLGVGGDLGLGVSRQVALGV
                     WFDYASYDARNRCGDCSASTWGLGGFARYHLMQGFRFDPWLQLGAGYRTFPVKNAGVS
                     FDYSGIEWLRLGLGGDYYVLSQLGVGPYAELSLGTLTKRPEPQDRSIYGVFSAGLRLV
                     FDASGR"
     gene            complement(2012..2503)
                     /locus_tag="VFQ61_36930"
     CDS             complement(2012..2503)
                     /locus_tag="VFQ61_36930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960152.1"
                     /translation="MSGESKGNSVSTEIGDSLGQLLGKEVSVTAIQTPREPSEIVVRY
                     VDGASELISAWTIERSLAWALGAALTMIPAEIVEEAIKSGTPQQELDDNFREVVNVLA
                     TVPANLLHRRSVLESVSSEKPALQRTARQVSAVDSSWVVLKVAVDGYAEGLLAVALLA
                     HQG"
     gene            complement(2519..>2741)
                     /locus_tag="VFQ61_36935"
     CDS             complement(2519..>2741)
                     /locus_tag="VFQ61_36935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960153.1"
                     /translation="LGLPLFVKGVVQPSDRIEIWTTEMRLAGIGVTVLLASPRECSAS
                     DHVQEVVKSLGRVMSIRAASREMANGKRL"
BASE COUNT          475 a          945 c          835 g          486 t
ORIGIN      
        1 gcgcggcacg ccggccgcgc gagccgccgc gctgacattg ccttggttac tcttgagtag
       61 ctcttgggct tgaccaaggt cgaggcgacc gacgcgggct acgtcccgtt ttccgctcag
      121 gccgagatgt tcgggcagga tgtacccggt gtccgcctgc atcgcggctc gatataggac
      181 tccgccgagt tctctgatat tgcccggcca atcgtgctct agaaggcgcg ctagagcagc
      241 gctggtcaag cgtttgtccc cgacctccga gcgcatccgg tcgagccaaa cactggcaag
      301 ctccgcgata tcggccttgc gctgccgtaa cggtgggatc accaccacga ccatcgctaa
      361 acgatgaaac aggtcggcgc gaaatcggga cgcttccacc cgctcctcca aacaagccca
      421 gctcgcggag atcaggcgta catccacgcg actcacctgg gtggcaccga gcgcccgcac
      481 gcagccatcc tctacgacgc gcagcagctt cacctggagt gcagctggca aatccgcgat
      541 ttcgtccaag aacaacgtgc cttgatgcgc ctgttcgaag gcccctaccc ggctctgaaa
      601 ggcgcccgtg aatgcgcccc gccgagcccc gaacagctct gcatcagcga gagattctgg
      661 caccgcgccc acgttgagcg gaacgtacgc tccggagcgg ccgctgagct gatgaatggc
      721 acgggcgact acgtctttcc ccgttccgga ctcgccttgc aaaagcactg gggcccgggt
      781 cgtcgcgtgc cgccgaactt cggaccaaac tttgcggatg ccgagagagg agccgaccag
      841 ccccgggacg ggttccgcat tgacctcact ttcatccgcc cccgcccgca aagcgacgct
      901 ggttcgcccc accacgaacg cggccgcctc gcgcaaacaa gcacgagcta cccgcacact
      961 tcccacgtaa acgccgttct tggagccaag atcctcgagc tccacgtgct cctccgtgac
     1021 tcggatgctg caatgcctct ggctaacggt cgaatcggcg acacgcagat ccgcgttccg
     1081 accgctgccg accactagtc gatcgccggc cgcgagtacg cgcgcgtggc cgccgtctgg
     1141 cgaacccact tccaggagcc acggcccgcg gggccggtag ctgcgaagtt cctcggtttg
     1201 cttggtttcg acgcaagaag gacggagctc tgattccatt gccctctatc ggatcgaacc
     1261 aacccgaagt ttccggtttt tcgcacggcc atgcgtgcta acaccggccc gcatgaacgc
     1321 ccgcacgctg ctgatcgcgg cccgactctc ggtcccaata ggactcactt cgagcctggc
     1381 gtgggcgcag ggcgcgcccc caaccccccc gcgcgagagc gatgagagtc acctcgcgcc
     1441 aattccgcgc gccaaggatc tgttgagcgg gcacgtgctg gtcggtgcga gcggccggtt
     1501 cgtcgttccc ttcggcgagt tcgacgattc gaccgatgtg cgagacatcg cccgccccag
     1561 cctaggcgtc ggcggggact tggggctcgg agtcagtcgg caagtcgcgc tcggtgtttg
     1621 gttcgactac gcgagttacg atgcgcgcaa ccgctgcggt gactgcagcg cctccacttg
     1681 ggggctcggt ggcttcgctc gctaccacct gatgcaagga tttcgtttcg acccgtggct
     1741 gcagctgggc gcggggtacc gaacgtttcc ggtcaagaac gccggggtct ctttcgacta
     1801 ctcggggatc gagtggctgc ggctaggcct cggcggagac tactacgtgc tgagccagct
     1861 cggagtgggc ccctatgcgg agctctcact gggcacgctc accaagcgcc ccgagccgca
     1921 ggaccgctcg atttacggcg tgttctccgc aggattgcgg ctggtgttcg acgccagcgg
     1981 gcgctgaaac acctactcgg gcgcgacgcg ctcagccctg gtgggcgagc agcgcaaccg
     2041 caagcagccc ctccgcgtag ccgtcgacgg ctaccttcag cacgacccaa ctagaatcca
     2101 ccgcgctaac ctgacgggcc gtgcgctgca gggccggctt ctcgctgctg acggactcga
     2161 gcaccgagcg acgatgcaag agattcgccg gcaccgtcgc gagcacattg accacttcgc
     2221 gaaaattgtc gtcgagttcc tgttggggcg tgccgctctt gattgcttcc tcgacgatct
     2281 cggcgggaat catggtgagg gcagcgccca atgcccaagc cagcgagcgt tcgatcgtcc
     2341 aggcggagat caactccgaa gcaccgtcca cgtaacgcac cacgatctcg gatggctcgc
     2401 gcggggtctg gatcgcggtg acggacactt ccttgcccag cagctggccg agcgagtcgc
     2461 cgatctcggt gctgactgag tttcctttcg attcgccgct cacgcgcgtg ccctcggttc
     2521 agagccgctt gccgtttgcc atctcgcgcg aggccgctcg aatcgacatc acgcggccga
     2581 ggctcttgac cacctcttga acgtgatcgg atgcggaaca ctctcgaggg ctcgcgagca
     2641 gcaccgtaac cccgatgccc gccaggcgca tctccgtggt ccagatctcg atgcggtcgc
     2701 taggttggac cacgcccttc acgaacaacg gtaagccgag c
//
LOCUS       DASTKU010001042         4192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_427833, whole genome shotgun sequence.
ACCESSION   DASTKU010001042 DASTKU010000000
VERSION     DASTKU010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427833"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            468..1085
                     /locus_tag="VFQ61_36940"
     CDS             468..1085
                     /locus_tag="VFQ61_36940"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9960154.1"
                     /translation="MTNLAFKPVSPSKSGSGPTDAALVIAARGGEAWAQQALFERYAR
                     LVLGLSHRILPHPADAEDLAQDVFVHALNRLDSLQNPQAFASWISSIVVRTASKRLRT
                     RRLLVRLGLRRDQPVDLESIVASGAPPEAAIELRAVYSVLHRLPAQERVAIVLRRVEG
                     LELTEVAEQMGLSLATVKRRLARAEARLLKLRPESAAPGVSRVMP"
     gene            1082..2350
                     /locus_tag="VFQ61_36945"
     CDS             1082..2350
                     /locus_tag="VFQ61_36945"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9960155.1"
                     /translation="MKSELSKYVALDLSSARVARLWPQIEPRVRLPRRHRPWLMRGAL
                     VGALALGVGAGALVVRSQSGAESVFQNAVLETQSDRLSVNLGEGSNLELSAETRVSVR
                     ESAAHAVRLRLERGSVVCDVSHLPGRHFSVEASGVEVRVVGTRFRVTQAPGRVQVAVE
                     RGAVEVRPHQSKAGNEYDSLKETTVHRISAGESLELPALPSTVLPSTALPSTPNGTAP
                     LARPTSSNSVVTGSVVNDSAVSKSAVEPRRSGNSLAERTPHLASRGNEGPQTSGSARS
                     SEQPAAAESVSTSSNLQRAGAAELLEIANAARRGGDLVGAARAYEALLSRFPADARSG
                     LAAFELGRLRLDRLGNPRGAVQAFERAIALAPGAGFREDAMARLVDAYSALGAVERCK
                     AARKSYLNSYPNGVRATAMAVKCGSADQPK"
     gene            2553..3473
                     /locus_tag="VFQ61_36950"
     CDS             2553..3473
                     /locus_tag="VFQ61_36950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960156.1"
                     /translation="MEFQAGPFSPAFEAQVLSDFRAGLAEEKIAICHSQDPGPEMPLA
                     TVRVSAESASNVSVSVDVRDAVTEKRVGRDVDLSRVPRDGRAFAVALAADELLRASWA
                     EVILKRAQSRGRRAPAAVRVAVERVLPSPLVKDHERPRVGVRGAVEHYGRGLTLYGGD
                     IALETTWFESILLDVHVGGRSAVERKAAHGTIAGSALELGLDLGGRWLWGNARAVRLA
                     WFGGVDASWLTFRGSAAGTARDYDWSGLGLAVHAGGGAGVRLVGPVWFEARASLGATV
                     RTLEALDGTLVAASASGLKSSLAVGIGVEL"
     gene            3470..>4192
                     /locus_tag="VFQ61_36955"
     CDS             3470..>4192
                     /locus_tag="VFQ61_36955"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960157.1"
                     /translation="MTLSSGQRGAPVHSWPYLFAAALCYAVCACTAQGIVLEAAGDST
                     EPLSFSTGAEHTCMVRSGIVYCWGRNTVGQLGRAASERETSPLPVGNSWQASNVTVGE
                     RHSCALSSTGRVYCWGGNDHGQLGDGQRTDRSEPGLVALPSPATSVSTRFNHTCVVLD
                     DSSAHCWGANFEGQLGQGGIGAAEDDGSLDALSPAPLSGRYVSVATGQGHSCGVRIDG
                     TLWCWGRNTAHQTSGGPEDQLRL"
BASE COUNT          684 a         1219 c         1467 g          822 t
ORIGIN      
        1 tggtaactct ggttaactct ggggctgtga gttgagttcg aaagatcagg ggcgagtcgt
       61 aacagtttcg agtgggtcga tctctcgagg tgttggcgct tcactcgctg cagcgttgtt
      121 ccgcgcggga cttggctctg cgggtagctg ttgggcctag tcacatttca caggcgggtt
      181 ccacgagagt ggtcgcctac aaggtaggga gtggtcggaa gtcctcgcca ccgccatgct
      241 gttcgtttcc gtttcgctcg aggaggccag tgacatcgag ccctatccgg ttctatttct
      301 accggtgctg gtgggtcgag gtaaaaacgt gagccggatt ggagtcgtcg cgggacatcc
      361 accacgtcga ggccgtctag gttcgacgat gcagcagtca tggcagcttc ccatcacgaa
      421 caaagacact cagcggcagg agctagccag cgccggacgt gccggcaatg acgaatttgg
      481 cattcaagcc cgtctctcca agcaagtcgg gcagcggccc gacggacgcg gcgctagtaa
      541 tcgccgcccg gggcggcgag gcttgggcgc aacaggctct attcgaacgc tatgctcggc
      601 tggtactggg gctatcgcat cgaattttgc cgcacccggc ggacgcggag gatctcgccc
      661 aggacgtctt cgttcacgcg ctgaatcgcc tggattcgct gcagaatccg caagcctttg
      721 cgtcctggat ttcttcgatc gtggtacgca cggccagcaa gaggctccgc acgcggcgcc
      781 tgctcgtgcg gctcgggttg aggcgagatc agcctgtgga tttggagagt atcgttgcgt
      841 cgggagcccc gcccgaagcg gcaatcgaac tgcgcgccgt ctattccgtg ttacaccgct
      901 tgcccgcgca ggagcgcgta gcgatcgtgt tgcgccgggt cgaaggtttg gagcttaccg
      961 aggtcgcaga acagatgggc ttgagcttgg cgacagtcaa acggcggttg gcgcgcgctg
     1021 aggctcggct cctcaaactc cgtccggagt cagctgcacc cggagtcagc agggtgatgc
     1081 cgtgaagtcc gagctttcga agtatgtggc gttggatctc tcgagcgcgc gggttgcgcg
     1141 gctgtggccg cagatcgagc cgagggtgcg tctccccagg cgccatcgtc cttggctgat
     1201 gcgtggggca ctggtcgggg cattggccct gggtgttggc gccggagcat tggtggttcg
     1261 atcgcagagc ggtgcggagt ccgtttttca gaacgccgta ctcgaaactc agagtgaccg
     1321 gctgtcggtg aacttgggag aaggatctaa cctcgagttg tctgctgaaa cgcgggtttc
     1381 cgtgcgcgag agtgcggcgc acgcggtacg gcttcggctg gagcgaggta gcgtggtgtg
     1441 cgatgtgagc cacctgcctg gacggcactt cagcgtggag gcttccggcg tggaagtcag
     1501 agtcgtcggc acgcgctttc gcgtcaccca ggcgccgggg cgcgttcagg tagcggtgga
     1561 gcgaggggcg gttgaagtca ggccccacca atccaaagcc ggcaacgagt acgactccct
     1621 caaggaaaca acggttcatc gcatttctgc gggagagagt ttggaactcc cggccctgcc
     1681 ctctacggtc ctgccctcta cggccctgcc ctcgacgccg aacgggactg ccccgctggc
     1741 tcgtcccact agtagcaaca gtgttgttac tgggagtgtg gtcaacgata gcgccgtcag
     1801 caagagcgcc gtggagccgc gtagaagtgg caactcgctg gcagagcgga ctccgcacct
     1861 tgcttcgcgc gggaacgaag gtccacagac ctcgggaagc gcacgaagct cggaacaacc
     1921 cgctgccgcg gagtccgtct cgacctcttc gaatcttcag cgggcgggtg cggctgaact
     1981 attggaaata gcaaacgccg cgaggcgggg cggagatttg gtgggagcag cgagagcata
     2041 cgaagcgttg ctctcgcgct tcccagcaga tgctcgctcc ggactggctg cattcgagct
     2101 cggacggctg cgcttggatc gactcggtaa tccgcgcggg gctgtgcagg ctttcgagcg
     2161 agcgattgcg ttggctccgg gagcgggctt ccgggaggat gcgatggctc gtttggtcga
     2221 tgcctattcg gccctgggcg cagtggagcg ctgtaaggcc gcgcgcaaga gctacctcaa
     2281 cagctatccg aatggagtac gtgcgacggc gatggcggtc aaatgtggct ccgcagacca
     2341 accaaagtag cctctaagac agtaacgctc ggcgccgcgc gtcggaacgc gtggcacagg
     2401 cgttctgggc tcgcttgttg gatggcgctc ggataggttg gcggagcgtg cgagttctgc
     2461 gagcgttttc ggctggaatc gtggtttgcg ctgtgcacgc gttctgcccg ctcgcccggg
     2521 ggtgcgacac gcgggggcag ccctggatcg ctgtggaatt ccaagcgggg ccgttctctc
     2581 ccgcgttcga agcgcaggtg ctgtccgatt ttcgcgccgg tctcgccgaa gaaaagatcg
     2641 ccatttgcca tagccaagat ccagggcctg agatgccgct ggctaccgtt cgggtgagcg
     2701 ccgaatctgc gagcaacgtc tcggtcagtg tcgatgtgcg cgacgctgtc accgaaaagc
     2761 gcgtcggaag ggacgtagat ttatcacgag tgcctcgaga cgggcgcgcc ttcgccgtcg
     2821 cgttggcagc cgacgagctc ttgcgagcca gttgggcgga ggtgattctc aaacgagcgc
     2881 agtcccgcgg gcgtcgcgct cccgccgcgg taagagttgc cgtcgagcgt gttttaccga
     2941 gcccgctcgt aaaagatcac gagcggccac gggtgggtgt gaggggtgcg gtcgaacact
     3001 acgggcgcgg gttgacgctc tacggtgggg acattgccct ggaaacgacc tggttcgaga
     3061 gcattctgtt ggacgtccac gtgggcggtc gcagcgcagt tgaacggaag gctgcgcacg
     3121 gcacgattgc cggtagcgcg ctcgaattgg ggctcgactt gggtggacgt tggctgtggg
     3181 ggaacgctcg cgccgtgagg ctcgcctggt tcggcggtgt ggacgcgtcc tggctcacgt
     3241 ttcgagggag tgccgccggt acggcgcgcg attacgactg gagcgggctt gggctggccg
     3301 tgcatgcagg gggcggcgcc ggggtacgtc tggtggggcc ggtgtggttc gaagctcgcg
     3361 cttcactcgg ggccactgtt cgcactctcg aagctctcga cgggacactc gttgccgctt
     3421 cggcttcggg tctgaagtcc tccctcgccg taggaatcgg ggtggagtta tgactctatc
     3481 ctctgggcag cgcggcgcgc ctgtccactc gtggccttac ctcttcgcgg ctgcgctctg
     3541 ctacgcagtc tgcgcatgca cggcgcaggg aatcgtgctc gaggctgctg gcgactccac
     3601 tgagcccctc tcattcagca ccggcgccga gcacacctgc atggtgcgtt ctgggatcgt
     3661 gtactgctgg ggtcgaaaca ccgtcgggca gctcggccgc gccgcttcgg agcgtgagac
     3721 atcgcccctt ccggtcggca actcgtggca agcctcgaat gttacggtgg gagaacggca
     3781 ttcttgtgcg ctgtccagca cgggtcgcgt ctactgctgg ggaggcaacg accacggtca
     3841 gctcggagat ggtcaacgga ctgaccggag tgagccaggt ctggtcgcgc tgcccagccc
     3901 cgctacttca gtgtcgacgc gcttcaacca tacctgcgtc gtgctagacg actcctcggc
     3961 ccattgctgg ggcgccaact tcgaggggca gctcggccag ggagggatcg gagccgcgga
     4021 agacgacggc tctctcgatg ctctgagccc ggcaccactc tcgggccgct atgtgtccgt
     4081 agcgacgggt caggggcaca gctgtggcgt acgcatcgac gggaccctct ggtgttgggg
     4141 ccgcaatacc gctcatcaga cttcgggcgg gccagaagac cagctccggc tg
//
LOCUS       DASTKU010001043        11388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1323670, whole genome shotgun sequence.
ACCESSION   DASTKU010001043 DASTKU010000000
VERSION     DASTKU010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11388
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323670"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(455..1075)
                     /locus_tag="VFQ61_36960"
     CDS             complement(455..1075)
                     /locus_tag="VFQ61_36960"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET9960158.1"
                     /translation="MNALKDEDYEAWLVDLDGTLYWAPGVKLAMGLELALFGLSKIKR
                     LRRFRHEHEALRAAGPAAALGDPFQLQLQSAAHALELEPAILEQDIRSWMIERPARYL
                     SWFKRKELLTRIRRFRAKGGRTALVSDYPATRKLEALGASDLFEVIVASGEPFGPKRL
                     KPDPSGYLTAASKLGVEPARCLVLGDRQDADGLAAQAAGMTFRKIP"
     gene            complement(1134..2015)
                     /gene="epsC"
                     /locus_tag="VFQ61_36965"
     CDS             complement(1134..2015)
                     /gene="epsC"
                     /locus_tag="VFQ61_36965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HET9960159.1"
                     /translation="MVLRNGELEGVIDNVVKSYDGHEEINNLDTAALPNKRAVVEAYK
                     HLRPVLFAGFYSVRSLDRDNLRFAISEHLYPAYETLVEQIGRALAYEERFHGVQHPPG
                     FAEEVVLSLFRRLPEVRLTLNSDVTAAFEGDPAAKSVEEIVFSYPAVHAITAHRIAHE
                     LHLASVPIIPRIIAEYAHSRTGVDIHPGARIGSHFFLDHGTGVVIGETSIIGDRVKIY
                     QGVTLGALSTRRTECSYGTRAYKRHPTIEDNVTIYAGATILGGETVIGAGSVIGGNVW
                     LTQSVPPNSKIFGRARE"
     gene            complement(2113..3750)
                     /locus_tag="VFQ61_36970"
     CDS             complement(2113..3750)
                     /locus_tag="VFQ61_36970"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HET9960160.1"
                     /translation="MTLVRTQYVSLAALSLVVFAWSTQASAQNTVAPPPPAPVTPPPA
                     GPAPTPAAARAPEATPPSASAPPAPASFPNPPPAAEGSSYVAPGYVAPTYAAGPPGTA
                     PGTYAPPAFIPTPPPPGNQVPPPAPVSAPTVISAPPEAAAPAWYDGFTIGGFADAYAA
                     VDFNFPKAPGQTRYNPVRAYDTKSGFSLSWVGLDLQKEAKPVGGTLALRFGPTAQTLA
                     AACPDLDTCDADSSLGLSYVKQAFVSFKPGGSESSVTLDLGKFDTPYGVEVAESQFNV
                     NYTRGILYWLGQPAYHTGLRVGVDASRNLNLKFLAVNGWNRTVDNNAGKTFGLQGTYR
                     LPRAPGAVEDMLTISVGYLVGPEHGDSKTIVCQPGQRFDLSANPQTGCVTDSRSTTEQ
                     TVVVDRGEANTKGLRHFLDLAVVASPTSALKLLFNASFAVDNDRELADTSKFTSSSWF
                     GVMGGARYALSDAFGLAGRIEYYADPDGVTSGHAGQDLRFVSGTGTLDYSVAQRLRLF
                     LDARLDWSSKKAFPVAAHDPNRGTSVSAALGAVFMTN"
     gene            4056..8138
                     /locus_tag="VFQ61_36975"
     CDS             4056..8138
                     /locus_tag="VFQ61_36975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960161.1"
                     /translation="MLLVRLRASGPMRVAWLSLIASTHLACGDEGRPSALLGDVTPGF
                     QSGRCSRPEAGCACSATEPVACSYMESRDAQSAICAEGQRTCSAGVWGECVAQRRVKK
                     YAPPAPADQQGQDEPSSPSGLRLSALASSAARCGDPCQPDCWVFKDSSTGLAPPAGLV
                     GNGTGITLPSRAAANTCSDLRVEASADTITVKSFEPLATEPAFVSFTARCGPGGEQIF
                     PTWAVDGAPHNATIDQNGTLTVLAPIAGNVEVRAINRQTSSARTLTIRVDVEEIDPDC
                     ADIAEDFTTRASALIDPGKTLYPYSVPARPVVFPLGLAAPLVQWANGGVASSCVRVGL
                     RFPSGAAAPDFSYAKYVSGEPRQGLVAGNEQPALQIPSAAWSVFDRTAKGADAEIFVQ
                     RRSDEASPRVFDEMSIPLRFADEPLRASLYYTQYLRRFRSDSGASTPICSGSRQTEIS
                     PASYPRTLAESGCLSADCEPNVGPVCPAGNCTQAKNVAGAASLQRLDLRSAGSAPEEP
                     FGSSATCTACHSLSANGKLLAAANRKYAGGATLTNVGDELFEPYAAPPTYSYRSSDDE
                     DTSPESEQSRGFSLGALSPDGRYVLQAPNFWGTTESHVTLAQGTNLRDAGYSAGGKRA
                     FLLDVRKLRAPVNYATIAPLPDHVRTKTQLIGDEEIPLAVDGSYVVVGDSVLIKDSTD
                     ATENGVYTVARSGGVGSTWLLERRSDSDQSGDLVFGQKFHVEAGTTLRNRTFYVAVPS
                     TGVIDPGATPLRFALYTTATAATTTILPPYTINGFDLTGSGPLPASYIDDVEPAVGLS
                     VLVKDEEGQNSRNNGIFRFTSLDPWVLTRRSDTNSSVEFQDLLRVRVNQGTRNGGRAF
                     TLRAPGPVELTEDGLIFDEETNLNEGLVFGGRTLPNLMYPTFSPNGQHLAYVSGAADG
                     LDTGWRRGLNILDFDPSTPAFSNRRLILNRFTAGMAGNVLKWPAFEPDSHSLLFVESS
                     PDEFCPLEASQGRCSSITRSASGHEVCTASTETLHVDTDLERACYQEPSTLGYGSASP
                     MARGYWPGRLHSIDVETGAASDRALDFLNRGQLAADADKVYQPSVLPVASGGYRWVVF
                     SSHRSYGNQFNQVGTHFSCGSSTLWIAALDDREADAGDRSYPAFLVPGQRLSGIEDPD
                     ENRHYLNERGVLVPAACLSEGSECTSDVDCCGGSGANPTSACRPDYPESEPVSHCRAL
                     SEACRVPGESCGADRACCAGLVCQAGACLSAQFYDRGVFEREFVGECPVGYKPRWLDF
                     EWHASATADSKILFTARVSADGDFSDASSVTLAQADSENQNPPPAGVQRVSVGDALWQ
                     SDIASGSHLKVRMEFVPSSDGRAAPTLFDWAQRYDCVALE"
     gene            8251..9297
                     /locus_tag="VFQ61_36980"
     CDS             8251..9297
                     /locus_tag="VFQ61_36980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960162.1"
                     /translation="MVFGFGFAITACSSDDEHPVAPSSSAGSGAIAGALVLGGQANSG
                     GRMPAAGVASVAGAGGASGESQGGETGESGAGGQSPLPPPVPYLCDPTTAFAGLEPLT
                     RISTDEDDLILGLSSDENTLVFAAGQAIYRSERNSRTGEFQTSSRIEAAEFVFDPASI
                     TLSSDGLRLIGVLADRPAFAELTRSSRVEEFGALPDETPFTTINSSAAGLLGRAMYAD
                     PVLSENDERLFYSSFDEGPNAVSTVFESLREEDSESWPAGDPSSYVGLFAQAGALRRP
                     RSLSGDGRTLFYWDEVSRQLRAAYRRTIDLPFEFSIEVGDYQRAIANHVCDRLYVSAA
                     SERGLDLFVATREQ"
     gene            9506..>11388
                     /locus_tag="VFQ61_36985"
     CDS             9506..>11388
                     /locus_tag="VFQ61_36985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960163.1"
                     /translation="MRSSFRIFSKCALLTALSVGQLTGCFGGSSDQPTVENQQAGSLI
                     LPLQAVGQSGNVYRLRNAFFDIFNLQTGESILLSSEDFVSSFEVTTQLVRGDYNITLL
                     DGWRMEQVTGSGGSGGSGGFGGGSPAGAAGAEAGGVAGEFVDPFPVPAGGRVITPPPR
                     PVPVPVPLPPIIDEGSAGDPGIAGAAPEPEGGTVGFGGASGIAGGISRGGAIGKGGTV
                     GVSGFTGTAGKDPTGGAPNSGVFVEALLLSPASQFFTIVARQDSFVNFQFAVGKDVIR
                     MNPGTLHIGFTVDEQCVRPPGAFDPNRVLLEHNRDAMQQVALRDVFTALATNDGRSAD
                     PVRLYQEVIDSFASKARGRLPDAIHCGDESVDGSPSLNGFPLVCDRTEAGQIDNIDTW
                     KPLAFVNRMDLAPQNGAHCGQQRMIFSTNAQNRMFMIFESQIPNPNPELGIQGCVPLA
                     KFWSEANGMTDVKARGARLVQAFLKGDPGLSSQGFGPFMTATNLTVGSGQIRTNSFDS
                     SPWTLREFKLAIEEQRLAAIPFPVAEAPNGDLWDENVPLAQGPSCRESFIQAAQGLLV
                     NNPSDMSFVVDQACKDAESQNNGSQNYAFRMSPGFRQTLQDAFAGTGLRAEDIATRAQ
                     FAGS"
BASE COUNT         2063 a         3386 c         3770 g         2169 t
ORIGIN      
        1 ccgcttgcga cgacgtgata agtcgtggcg ccgggctgcc cccaaggcac tgcgcgcggg
       61 gccccagcgc cgctgcgatg acgtgatagg tcgtggcgtg agttcgcggg gctgccccaa
      121 gggacagcgc gcggggcccc agcgccgctg cggcgacgtg ataactggtg agagtgaaag
      181 accgctggga tctccaggcg gaagcacagc gcgcggggct ccagccgcgc ttgcgaggac
      241 ctgataactg ggagtgaatg gcggcgggtc ctgccgccta accgggtgct gctgaattcg
      301 cagtggagga tttgtggtca gccgacgggg ttacgagcgc gaggagtggt ggtgggcgcg
      361 ggagcgcagt cgagaagcgt ggtggagcac gcggagccgc gcttgggcca ctctgtaacg
      421 catgcggcgt gacgcgcggg tctcgggggt ggcgttaggg gattttgcgg aaggtcatgc
      481 cggcggcttg ggcggctagg ccgtcggcgt cttggcggtc gccaaggact aggcaacgcg
      541 cgggttcgac gccgagtttg ctggccgcgg tgaggtagcc agaggggtcg ggcttgaggc
      601 gcttggggcc aaagggttcg ccactggcca cgatgacttc gaatagatcg ctcgcgccga
      661 gggcttcgag tttacgcgtc gcggggtagt cgctcacgag cgcggtgcgc ccgcctttgg
      721 cgcggaagcg acggatgcgc gtgaggagtt ccttgcgttt gaaccagctc aaatagcgag
      781 cagggcgctc gatcatccag gatcgaatgt cttgctccaa aattgccggt tcgagctcca
      841 aggcgtgcgc cgcgctctgg agttgcaact gaaagggatc accgagcgcc gccgcgggtc
      901 cggcggcgcg tagcgcttcg tgttcgtggc gaaagcgccg gagtcgcttg attttgctga
      961 gtccaaacag cgcgagctcc aagcccatcg cgagcttgac gccaggggcc cagtacaggg
     1021 tgccatccag atccaccagc cacgcttcgt agtcttcgtc tttgagggcg ttcatggttt
     1081 tcggccaatc gggtgtacgt caggctggta tcactcgggg gcttgcatcg gcttcattcg
     1141 cgggcgcggc cgaagatctt ggagttgggt ggcacggatt gggtgagcca aacatttccg
     1201 ccaataaccg agcccgcgcc aatcacggtc tcgccaccca ggatggtggc gcccgcatag
     1261 atggtgacgt tgtcttcgat ggtgggatgg cgcttgtaag cgcgagtacc atacgagcac
     1321 tcggtgcgtc gggtgctgag cgctccgagc gtaacaccct gatagatctt cacgcgatct
     1381 ccgatgatgc tggtctctcc aatgacaacg ccggtgccgt gatccaagaa gaaatgggaa
     1441 ccgatgcggg caccagggtg gatgtccacg ccagtccgtg agtgagcata ctcggcgatg
     1501 attcggggga tgatggggac ggatgctaga tgcaactcat gtgcaatccg gtgcgcggtg
     1561 atggcgtgca cggccgggta gctgaacacg atttcttcga cgctcttcgc ggcggggtca
     1621 ccctcgaatg ccgcagtgac gtccgagttc agcgtaagcc tcacctcggg caggcgccga
     1681 aataggctca atactacctc ctcggcgaag ccgggcgggt gctggacgcc gtgaaaacgc
     1741 tcctcgtagg ccagggctcg cccaatctgt tcgacgagag tctcgtaagc gggatacagg
     1801 tgctcggaga tcgcgaagcg gaggttgtcg cggtccaagc tgcgcacgct gtagaaaccc
     1861 gcgaacagca cgggccggag gtgcttgtag gcttcgacta cggcgcgctt gttgggtaac
     1921 gcggccgtgt cgaggttgtt gatctcttcg tgaccgtcgt agctcttgac tacgttgtcg
     1981 atcacgcctt ccagctcgcc gttcctgagc accatgcctg agcttagcct gccttgacgt
     2041 ggcgggcgcg catcacggaa tgaacgacgc gcgcgtcgct cgattcatca tcaatgaatg
     2101 ctcgaccagc gctcagttgg tcatgaacac ggcgccgagt gcggcagaaa cggacgtgcc
     2161 gcggttcggg tcgtgagcgg ctactggaaa agctttcttg ctcgaccaat ccaggcgcgc
     2221 gtccaaaaat aggcgcaaac gttgggcaac ggagtaatcc aaggttcccg tgccggagac
     2281 gaagcgcaga tcctgccccg catggcccga ggtcacgccg tccgggtccg catagtattc
     2341 gatacgaccc gccaagccga aagcgtcgga gagggcgtaa cgggcaccgc ccatcacgcc
     2401 gaaccaggag ctgctcgtga atttggaggt gtccgcgagt tcgcggtcgt tatcgacggc
     2461 gaagctcgcg ttgaacagca acttcagcgc ggaggtcggg gaggctacga ccgccaaatc
     2521 caagaagtgg cgaagtcctt tggtgttggc ttcgccgcga tcgacgacca cggtctgttc
     2581 cgtggtgctt cggctgtccg tcacgcagcc tgtttggggg ttcgctgaga gatcgaagcg
     2641 ttgaccgggc tgacatacga tcgtcttcga atcgccgtgc tcgggcccta cgagatagcc
     2701 gaccgagatc gtcagcatat cttccacggc gccgggcgcg cggggcaggc gataggtgcc
     2761 ctgcaatccg aaggtcttgc cggcgttgtt gtccactgtg cggttccagc catttacggc
     2821 taggaatttc aaattcagat tgcgggaagc gtccactcca acgcgtaggc ccgtgtgata
     2881 cgcgggctgg ccgagccaat agagaatgcc gcgcgtgtag ttgacgttga actggctctc
     2941 ggccacctcg acgccatacg gcgtatcgaa cttgccgaga tccaacgtga ccgagctctc
     3001 gctcccaccc ggcttgaagc tgacgaacgc ctgctttacg tagctcagcc cgagcgagct
     3061 gtcggcatcg caggtatcca gatcggggca cgccgcggcg agggtctgtg ccgtgggacc
     3121 aaacctgagc gcgagcgttc cgccgacggg cttggcctct ttctgcaaat cgagacctac
     3181 ccacgacagt gaaaagccgc tcttcgtgtc gtaagcccgc accgggttgt aacgcgtctg
     3241 gccgggagcc ttcgggaagt tgaagtcaac ggccgcgtag gcgtccgcga agccgccaat
     3301 ggtaaatccg tcataccagg ctggggccgc tgcttccggc ggggccgaga tcaccgtcgg
     3361 cgcgcttacc ggagctggtg ggggaacttg attgccgggc ggcgggggcg tcggaatgaa
     3421 ggcgggcggc gcatacgtac cgggcgctgt cccgggcggc cctgctgcgt acgtcggcgc
     3481 gacgtagccg ggggccacgt aactcgagcc ctcagcagca ggaggtgggt tcggaaagct
     3541 cgccggcgcg ggcggcgcac tggccgaggg aggcgtggcc tcgggagctc gggcggcagc
     3601 gggtgtcggg gccggaccgg cgggcggcgg ggtaacgggt gcggggggcg gaggcgcgac
     3661 cgtgttttga gcggaagctt gcgtgctcca agcaaaaacc accaaagaaa gcgcggccaa
     3721 ggagacgtac tgcgtacgga ccagcgtcat gtgggaatcc tccgctccag aaacgggagt
     3781 cccccagtac catagccagc ctcttagcca aagaacctga tgtaacgaag ctcacagacg
     3841 ggcgctgctt cggagataag aaggaagaac ttcaggcagg ctcggaacac cgaagtttcc
     3901 attcagaaac gagctttgaa agcagcgttg agcgcgctga agcgggcata tttcgctcgg
     3961 cgcttcgggc tctccgcacc ggacgagcag gcctcccgtc aaggtgtgcc acaccgcacc
     4021 gcgcctcgag gttctcatgc taacttgggc cggtggtgtt gctcgtcaga ttgcgcgcgt
     4081 ctggcccgat gcgggttgcc tggttgtcgc tgatcgcatc gacgcacctc gcttgcggag
     4141 acgaggggcg cccgagcgcc cttttgggtg atgtcactcc cggttttcaa tccggccgct
     4201 gttctcgccc ggaagcggga tgtgcctgct ccgccaccga acccgtcgcc tgctcgtata
     4261 tggaatcgcg ggacgcccag agcgcgattt gcgcggaggg gcagcgcacc tgcagtgccg
     4321 gagtatgggg cgagtgcgtc gcccaaaggc gtgtgaagaa gtacgccccc ccagccccgg
     4381 cagatcagca gggccaagac gagccgtcga gtccgagtgg cctacggctc tcggccttgg
     4441 cgagcagcgc cgcccgttgc ggcgatcctt gccaaccaga ctgctgggtt ttcaaggatt
     4501 cctcgactgg tttggcaccg ccggctgggc tcgtagggaa cggcactggg atcacgttgc
     4561 cgagtcgagc ggccgccaat acctgcagtg atctgcgcgt cgaggcgagc gccgacacga
     4621 ttacggtcaa gtctttcgag cccctggcaa cagagcctgc cttcgtctcg ttcacggccc
     4681 ggtgcggccc cggcggggag cagatcttcc cgacttgggc cgtggacggc gcgccgcaca
     4741 acgcgaccat cgatcagaat ggcactctca ctgtgctcgc tcccatcgcc ggaaacgtcg
     4801 aggtacgagc catcaaccgg cagacgagca gcgctcgaac cctcaccata cgagtggatg
     4861 tcgaagagat cgatccggat tgtgcggata tcgcggaaga cttcacgacg cgagcgagtg
     4921 cgttgatcga tcccggtaag acactctatc cctactcagt ccccgcgcga ccagtcgttt
     4981 tcccgctcgg tttggccgcg cccctcgtgc agtgggcaaa tggcggggtc gcatccagtt
     5041 gcgtccgggt cgggttgcgt tttccaagtg gagccgccgc gccggacttc tcgtacgcca
     5101 agtacgtatc cggtgaacca aggcaggggc tcgtagcggg caacgagcaa cccgctctgc
     5161 aaatcccgag cgcggcttgg agcgtgttcg atcgcacggc caagggagca gacgcagaga
     5221 tcttcgtgca gcgccggagt gacgaggcga gcccgcgcgt gttcgacgag atgtcgatcc
     5281 cgctgcgatt cgcggatgag ccgttacgag cgagccttta ctacacacag tacctgcgtc
     5341 gattccgcag cgactcggga gcatctacgc cgatctgctc gggcagccgc caaaccgaaa
     5401 tcagcccggc gagttacccg aggacgctcg ccgagagcgg atgtttgagc gcagattgcg
     5461 agccgaatgt gggcccggtg tgccccgcgg gcaactgcac gcaggccaag aacgtggctg
     5521 gcgcggcatc gctgcagcgt ctcgatctac gtagtgccgg cagcgcaccg gaagagcctt
     5581 tcggtagctc ggcgacctgc actgcttgcc actcgctttc tgccaacggc aagctgcttg
     5641 ctgccgcaaa tcgcaagtat gccggaggcg cgacgttgac gaacgtcggg gacgaattgt
     5701 tcgagcccta tgcggccccg cctacttatt cctatcgcag cagtgacgac gaagacacgt
     5761 cgcccgagag cgagcagagc cgcggcttca gcctgggcgc gctcagcccg gatggtcgct
     5821 acgtgctgca agccccgaac ttctggggca ccaccgaatc gcacgtgacg ctggcgcagg
     5881 gcaccaacct gcgtgatgcg ggttattcgg caggaggaaa acgagctttc ttgctcgatg
     5941 tgcgtaagct ccgggccccc gtcaattacg cgaccatcgc gcccttgccc gatcacgttc
     6001 gcaccaagac gcagctgatc ggggacgaag agattccgct cgcggtcgac ggcagctacg
     6061 tcgttgtggg ggactcggtg ctgatcaagg attccacgga cgcgacggag aacggcgtct
     6121 acaccgtggc tcgctcgggc ggcgtgggct cgacatggct gctcgaacgc cgatcagact
     6181 cggaccagag cggagacctc gttttcggac agaagttcca cgtggaagcc ggaacgacgc
     6241 tgcgcaaccg caccttctac gttgccgtgc cgagcaccgg cgtgatcgat ccgggcgcga
     6301 cgcccctgcg cttcgcgctt tatacgaccg ctacggcggc gaccacgacg atcctgccgc
     6361 catataccat caacggcttc gacctcacgg gtagcggccc attgccagcc tcttacatcg
     6421 acgacgtcga acctgccgtc ggcttgagcg tgctggtcaa agacgaagaa ggccagaact
     6481 cacgcaacaa cgggatcttc aggttcacga gcctcgaccc gtgggtgctg acgcgtcgct
     6541 cggataccaa cagcagcgtc gagttccagg atctattgcg ggtgcgggtc aaccaaggca
     6601 cgcgcaacgg tggtcgagcg ttcacgttgc gcgctccggg tccggtcgag ctcacggagg
     6661 acggcctaat tttcgacgag gagaccaacc tcaacgaagg cttggtgttc ggtggccgca
     6721 cgttgccgaa cctgatgtac ccgacgtttt cgccgaacgg acagcatctg gcttatgtgt
     6781 cgggggccgc ggatggtctc gacacgggct ggcggcgcgg gctgaacatc ctcgatttcg
     6841 acccgagcac gcccgcgttc tccaaccgac gcttgattct gaaccgcttc acggccggca
     6901 tggcgggcaa cgtgctgaaa tggcctgcct tcgagcccga tagtcatagc ctcttgttcg
     6961 tcgaatcgag cccggacgag ttctgcccgt tggaggcttc gcaaggccgt tgttcgagca
     7021 ttacgaggtc cgcctcggga cacgaggtct gtacggcaag cacagagaca ctgcacgtcg
     7081 acacggatct ggagcgggcg tgctaccaag aacctagcac cttgggatat ggaagcgcct
     7141 ctccgatggc gcgcggttat tggcctgggc ggctgcactc catagatgtc gagacggggg
     7201 cggcgtccga tcgcgcgctg gacttcctca atcgcggtca gctcgccgcg gatgcggata
     7261 aggtctacca gccgagcgtg ctgccggtag cgtcgggcgg atatcgctgg gtggtgttca
     7321 gcagtcatcg ctcctacggg aaccagttca accaggtggg cacgcacttc tcctgcggca
     7381 gctccacttt gtggattgcg gcgttggacg accgtgaagc cgatgcaggg gaccgcagct
     7441 atcccgcgtt cctggtgcct gggcagcgtt tgagcggcat agaggatccg gacgaaaacc
     7501 gacattacct aaatgaacga ggagttctcg tcccggcagc ttgcctgtcc gaaggtagcg
     7561 agtgcacttc ggacgtggat tgctgcgggg gaagtggagc caatccgacc agcgcatgtc
     7621 gccccgacta tccggagtcg gagcccgtga gtcactgccg tgctctgtcc gaggcctgcc
     7681 gagtgccggg tgaaagttgc ggggctgatc gggcctgctg tgcagggctc gtctgtcagg
     7741 ccggtgcctg cttgtcggcg cagttttacg atcgaggcgt gttcgagcgt gagttcgtgg
     7801 gcgaatgccc ggtcggctac aagccacgct ggttggattt cgagtggcat gcctcggcga
     7861 cggcagattc gaaaattctc tttacggctc gagtctcggc cgacggcgat ttctcggacg
     7921 cgagcagcgt caccttggcc caagcggact ccgagaatca gaatccccca cccgccgggg
     7981 ttcagcgcgt ttccgtcggc gacgcgctgt ggcaatccga tattgcctct ggctcgcatt
     8041 tgaaggttcg aatggaattc gtgccctcga gtgatggacg cgccgccccg acattgtttg
     8101 actgggcgca gcgctatgat tgcgttgctt tggagtgacc aggaaagccc atctcggatc
     8161 gcagttactg gacaggctct aaagaaccgg gcgggggcgg ttttgcggta ggctggcccg
     8221 gtgacacgta ccggctcatc ctggcttctc atggtatttg gcttcgggtt cgccatcacc
     8281 gcgtgttctt cggatgacga acacccggtg gccccgagtt cgagcgccgg ttcaggcgcg
     8341 atcgccggcg ctcttgtcct ggggggacag gcgaactctg gtggtcgtat gccggccgcg
     8401 ggcgtggcta gtgttgcagg agcgggcgga gcaagtggtg agtcgcaagg gggtgaaacc
     8461 ggtgaatccg gcgcaggcgg gcaatccccc ctgcctcccc cggtgccgta cttatgtgat
     8521 cccaccaccg cattcgccgg cctcgagccc ctgactcgta tatcgaccga tgaagacgat
     8581 ttgattttgg gcttgagttc cgacgagaat acgttggtat tcgcagccgg gcaggcgatc
     8641 taccgttcag agcgcaattc ccgaacggga gaattccaga cgagctctag aatcgaagcg
     8701 gcggagttcg tcttcgatcc ggcttccatt accttgagta gcgacggctt gcgcttgatc
     8761 ggcgtgctag ccgaccggcc agcttttgcg gagctgactc gatcatctcg agtcgaagag
     8821 ttcggggcgc tgccggacga gactcctttt acgaccatca actcgtctgc agcgggctta
     8881 ctgggccgcg ccatgtatgc ggatccagtc ctttccgaga acgatgagcg tttgttctat
     8941 tcgtcgttcg atgaaggccc gaatgccgtg agcaccgtgt tcgagagcct gcgcgaggag
     9001 gactcggaaa gctggcctgc gggtgacccc tcgagttatg tcgggctgtt cgcgcaagcg
     9061 ggcgcactgc gtcgacctcg ctcgctgtcg ggcgacggac gaaccctgtt ctattgggac
     9121 gaggtcagcc ggcagttgcg cgcggcatac cgaaggacaa tcgacttgcc gttcgagttc
     9181 agcattgaag tgggagatta ccaacgcgcg atcgccaacc atgtttgcga tcgcttgtat
     9241 gtctccgctg cctccgaacg cgggctcgat ctgttcgtcg ccacgcgtga acagtgaatc
     9301 cagagcacgc cgtgaatatc agatcacggg atgtgaacta cgggactgac accgtttaga
     9361 gtcttcacca gagcggtgtg ccaacgcggg tggcacatga cgctcgtgga tactcggtta
     9421 tctcgaagaa tatcgacgaa tgacaatggc gtgaaacatg cagttagtgc gctgccgtcc
     9481 ttcgggaggt tgcgaggagg ttctcatgcg atcgtcattt cgtatttttt ccaagtgtgc
     9541 cctgctcact gcgttatcgg tcggtcaact gacaggctgt ttcggtggct cgagcgatca
     9601 gccaacggtc gagaatcagc aagccggttc attgatattg ccacttcaag cagttggtca
     9661 gtcgggcaat gtctaccgat tgcgcaatgc gttctttgat atcttcaacc tgcaaacggg
     9721 agagtccata ctcctgagca gcgaagactt cgtgtcttct ttcgaggtca ccactcaact
     9781 ggtgcgtggt gactacaata ttacgttgct cgacggttgg cggatggagc aagtcaccgg
     9841 gagcggtggt tcagggggga gtggcggctt cggtggcggc tcccctgcgg gcgcggcggg
     9901 tgccgaggcg ggaggtgtgg ccggcgagtt cgtcgatcca ttcccggtcc ccgcgggtgg
     9961 tcgagtcatc acgccgccac cgcggcccgt tcccgtgccg gttccgctac cccccatcat
    10021 cgacgagggc agcgcgggtg acccggggat agccggtgcc gcgcccgagc cagaaggagg
    10081 aaccgttgga ttcggcggag ccagcggcat tgccggcggc atctctcgcg gcggcgcgat
    10141 tggcaaaggt ggcaccgtcg gtgtgagcgg gtttaccgga actgcgggca aggaccccac
    10201 cggaggagcg cccaactcgg gtgtgttcgt cgaagctctg ctcctcagcc ccgcaagcca
    10261 gttcttcacg atcgtggccc ggcaggattc gttcgtcaat ttccagtttg cggtcggtaa
    10321 ggacgtgatt cggatgaacc cgggcacgtt gcatatcggc tttaccgtcg atgagcagtg
    10381 cgtgcgcccc ccaggtgcgt tcgatcctaa ccgcgtgtta ctcgagcaca accgcgacgc
    10441 catgcagcaa gtggcactgc gagacgtgtt cacggcgctc gccaccaacg acggacgcag
    10501 cgcggatccg gttcgtctgt atcaagaggt catcgacagc ttcgcgagca aggcgcgcgg
    10561 taggctgccc gacgccattc actgcggcga cgaaagcgtg gacggcagcc cgtccctgaa
    10621 cggtttcccg ctggtgtgcg atcggaccga agcgggccag atcgacaata tagatacttg
    10681 gaagccgctg gccttcgtca accgtatgga cctcgcgcct cagaacggcg ctcactgtgg
    10741 tcaacagcgc atgatcttct cgaccaatgc gcagaaccgt atgttcatga tcttcgaatc
    10801 ccagattccg aatccgaacc ccgagctcgg aattcagggc tgcgttccgc tcgccaagtt
    10861 ctggagtgaa gccaatggca tgacggacgt aaaggctcgc ggtgcgcgct tggtccaagc
    10921 cttcttgaag ggagatcccg gcctatcttc acaggggttt gggccattca tgaccgccac
    10981 caatctcacc gtgggcagtg ggcaaatccg gaccaacagc ttcgactcga gcccgtggac
    11041 gctgcgtgag ttcaagcttg ctatcgaaga gcagcgtttg gcggccattc cgttccccgt
    11101 ggcggaggca ccgaacggtg acctgtggga cgagaatgtg ccgctggccc aaggtccgtc
    11161 gtgccgtgag agcttcatcc aagcggcgca agggctgctc gtgaacaacc catcggatat
    11221 gtccttcgtg gtcgatcaag cgtgtaagga cgccgagagt cagaacaacg gttcacaaaa
    11281 ctacgccttc cgcatgtccc ccggtttccg tcagaccttg caggatgcgt ttgcaggcac
    11341 gggccttcgc gcggaggata tcgccacgcg cgctcagttc gccggttc
//
LOCUS       DASTKU010001044         7254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_428108, whole genome shotgun sequence.
ACCESSION   DASTKU010001044 DASTKU010000000
VERSION     DASTKU010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428108"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1486)
                     /locus_tag="VFQ61_36990"
     CDS             complement(134..1486)
                     /locus_tag="VFQ61_36990"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9960164.1"
                     /translation="MALNEAQREYLKTFVAKYAARSPGSRERRERAWPHLADPRSSIG
                     FSQHAPAAVRELWLATKGLRYPIVAGRCQGEKVWDIDGNQYVDYGLGFGVNLFGHNPE
                     FIQRALHERIALGSPMGFQSECANEVAAGICALTGAERVAFCNTGTEAVMGAIRVARA
                     MTGRAKIVVFAESYHGSHDDVVGAIGATHGISPAHAAECLVLQYGTEESLQAIAQHSD
                     QIAAVLVEPVQARRPDLQPAEYLHRLRALTHERRILLVFDDVLLGFRIHPGGCQAHFD
                     IRADLATYGKVIGGGMPIGVLTGKSEYMDCIDGGVWRHEDESYPVRDKIWFAGTFNKN
                     PLTMATTRAVVDRLREAGSGLQDSLNRMAEQFATRLGQWLTENGMPVSIARFGSMFRF
                     KSSPELTLLIQHLHLRGIFTWEGMVFFLSTAHTERHLDELEQAVKDSLLTMRRGGYIQ
                     "
     gene            complement(2302..2871)
                     /locus_tag="VFQ61_36995"
     CDS             complement(2302..2871)
                     /locus_tag="VFQ61_36995"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET9960165.1"
                     /translation="MSKSHFVSHTPATAPAEARDYLTGAQQQFGFIPEPLARMVEAPA
                     LVEAFFKINPIFEKSTAFSPLEREVMVMTIGAYNGCAYCVAMHTAFLKRMEAPEKLIE
                     ALREQRTLTDPKLDALAKFTRRVLEHRGNVGDGAMAEFERAGYTARHALEVVLGVATY
                     VLTTYSNRMTKAPLDAPFESFRWQGAEAE"
     gene            3723..4862
                     /locus_tag="VFQ61_37000"
     CDS             3723..4862
                     /locus_tag="VFQ61_37000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960166.1"
                     /translation="MAGSYKAGSSKDSLQSSAAPKRLRNHWLLGSASAVLIGTIVSAC
                     SGMDQTASTSGAHSEAVGEQTDLLSDGSPAAGASATGSNAAAGNTAAGNRVEFCYRGE
                     QMVRPVSVYNANRSRGAYLGACDLVENRTSLGDGYVQTFIHRNSDGSLRAIGVQIDQV
                     AMDTLPMEPVNDGATCFDQDGDGELDPHMECSGGHERVLFFPKVDGLPISWIMFNWQS
                     MGHAPMNVFDKAHFDLHYFIQDYISRNFIRTGPCGTQVNCDDFDKAMRDIPAPFFPVG
                     FENVGGVAGRMGNHLVNKSEAPFNGEPFTQAFAYGEYDAHLSFFEPVVGTDYLKTRPN
                     TCGAIKPPPAVELSGLYPKKYCSRFRADRGDYIMTLEDFEYRAAP"
     gene            5343..6311
                     /locus_tag="VFQ61_37005"
     CDS             5343..6311
                     /locus_tag="VFQ61_37005"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET9960167.1"
                     /translation="MLRTRIIDEQVTGWQRQGRIRFHIGSLGEECCIIASACALRASD
                     WIFPSYREFGALLMRGMPLREYLGNLFGNAHDPAKGRQMPDHYTGKPFNFGCVSSPIA
                     TQIPQAVGFAYASRLRNEGAVIAAYFGDGATSSGEFHAGLTLAGVLKTPNVFLLRNNG
                     WAISCPNERQCAVRALSDKAHGYGIRGVRCDGNNAEETYLTVLEAVEHAAHGRGSTLV
                     EMVASRQGGHSTADDPRLYRSPEEEARGLKEDPVARLRQRLRLLEAWSDDEEHQLRQA
                     VAEELVEELREAELASPPTLEGLFDDVYAHLPEHLREQREKALRFA"
     gene            6331..>7254
                     /locus_tag="VFQ61_37010"
     CDS             6331..>7254
                     /locus_tag="VFQ61_37010"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET9960168.1"
                     /translation="MTELNLVQAVNAALRAEMRRDERVVILGEDVGKLGGVFRATRGL
                     YEEFGQARVIDTPLAENGIIGAAIGMALHGLRPVAEIQFSDFIYPAFDQIVSEAAKYR
                     YRSGGQFAVPMVIRAPIGGGVAGGMYHSQSPEALFIHTPGLKVVCPSTPYDAKGLLIS
                     AMRDEDPVLFFEPKRLYRGRPMPVPGEDYTIPLGRARVVRPGRDVTLVAWGAMVVEAF
                     ETAEELAPLGVDCEVIDLRTLWPLDLVSLEQSTKKTGRLLVVHEAPLTCGFGAELVAL
                     LSQRCFDYLRAPPERVTGWDIPFGYARELDYL"
BASE COUNT         1403 a         2132 c         2276 g         1443 t
ORIGIN      
        1 cagccggagc gcagtgtaga ccaagggggt aaacgcctga atacccaagc gtgagacact
       61 gagccccgta ccgaaacagg ctgcggaaaa caagatcgtc gcgaggcggc gcatgttcgt
      121 gttggaccgg ccgttattga atgtacccac cgcggcgcat ggtgagtagg ctgtctttga
      181 cggcttgctc gagctcgtcc aagtgacgct cggtgtgcgc cgtgctcaga aagaacacca
      241 tgccctccca cgtgaagatt ccgcgcaagt gcagatgctg aatcaacagc gttagctcgg
      301 gagaggactt gaagcggaac atcgagccaa agcgagcgat gctgaccggc atgccgttct
      361 cggtcagcca ttggccgagc cgcgtagcga actgctcggc catgcgattc aatgagtctt
      421 gcaggccgct gccggcttcg cgcagccggt caaccaccgc gcgcgtcgtg gccatggtga
      481 gggggttctt gttgaacgtc cccgcaaacc agatcttgtc gcgcacgggg tagctctcgt
      541 cctcgtggcg ccaaacacca ccgtcgatgc aatccatgta ttcgctcttg ccggtcagca
      601 caccgatcgg cattcctccg ccgatgacct tgccgtaagt ggcgagatcg gcgcgaatat
      661 cgaagtgagc ttggcatcca ccgggatgga tacgaaaacc caggagtacg tcatcgaaca
      721 cgagcagtat gcggcgctca tgggtgaggg cgcggagtct gtgcaagtac tccgcaggct
      781 gcaaatcggg gcgacgcgcc tgaaccggct ctaccaagac cgccgcaatt tggtcggagt
      841 gctgggcaat cgcctggagg ctctcttccg tcccgtattg aagcaccagg cactccgcag
      901 cgtgggccgg ggaaatgcca tgagttgcgc cgatggcgcc gacgacgtca tcgtgagagc
      961 catgatagga ctcggcgaat acgacgatct tggccctgcc cgtcatcgca cgcgcaacac
     1021 ggattgcgcc cattacggcc tccgtgcccg tgttgcagaa cgcaacgcgc tcggcgcccg
     1081 tgagcgcgca aatgccggca gctacttcgt tggcacattc ggactgaaac cccatcgggg
     1141 acccgagcgc gatgcgctcg tgcagggcac gctggatgaa ctcgggatta tgaccgaaca
     1201 ggttgactcc aaagccgagc ccgtagtcga cgtattgatt gccgtcgata tcccaaacct
     1261 tctcaccctg acaacgcccc gccacgatcg ggtaacgcag ccccttcgtg gcgagccaga
     1321 gctcgcgcac cgccgccggc gcgtgttgcg aaaatccgat tgaagagcga ggatcagcca
     1381 aatgtggcca ggcccgctca cgtcgttcgc gtgaccccgg actacgcgcc gcatacttgg
     1441 ctacgaacgt cttgagatac tcccgctgtg cttcattcaa agccatttgc ccgactcctt
     1501 gatttcagtc tcgcgttctc ttccaccacg cgcgcagagg cgcgcacgtt ctggcgacct
     1561 cgcaccggaa caaccgccaa tctcgtcgat cgtgccaaac tgactccgat ggaacgattc
     1621 acaccgcccg ccgcaccgag ctagtcaagc tcagcctata cgctttgatg cgctcttcca
     1681 cgtggatacg tggttcaagt gcacctgagt tccattgctc gttcgatacc tgctccggta
     1741 tcctgtttcg aggtttgatc ctgattccca aacgcggatc gagctgccga gtacgaactc
     1801 tgattctccc tcacacgggt gcagcgcaaa tcctctattc ccgactatct gggcgagcaa
     1861 gttccgagct ccgcgactca gcggacgcgc tccgggcggc acaaccgctc ttgggaaagc
     1921 acccgaaact ccccagctcg tttcgggtgc tccgccaaca gcccgctgcg ggttggtacc
     1981 accgtggaaa ctcgagacga ccaaaacacg cgcaccccaa agcaggagtt cgcagcgctc
     2041 agcaagaatt ggatacggag cacacgagct agcgcacgcc ccggaacaca ccaataaggc
     2101 cgaatcggag tccacagcgt ggactcagca ggtcctcgcc ttcctattga aaggccgtca
     2161 ggcacttcaa gcgccgcttg cgaccgtacg tggcgattgc tgaaccgctg gttcgagcgc
     2221 aaatcgggcc tgaccgcatc actctggatg tgacggctgc ggtcgcgccc tgcaacgacc
     2281 tttcactaac ccgcgatgtt gctattctgc ctcagcgcct tgccaacgga aggactcgaa
     2341 tggagcgtcc aggggagcct tcgtcatgcg gttggagtac gtcgtcagga cgtaagtagc
     2401 gacgcccaac accacctcga gcgcgtggcg cgccgtgtaa ccggcccgtt cgaactcagc
     2461 catggcgcca tcaccgacgt taccgcgatg ctccaacacg cggcgggtga acttcgcgag
     2521 cgcgtcgagc ttgggatccg tcaacgtgcg ttgctcccgc aaagcctcga ttagcttctc
     2581 gggcgcttcc atgcgcttca agaaggcggt gtgcatggct acacagtacg cacaaccatt
     2641 gtaagcaccg atggtcatga ccatcacttc acgctccaac ggcgagaacg cggtgctctt
     2701 ttcgaagatg gggttaatct tgaagaaggc ctcgaccaga gccggagcct cgaccatacg
     2761 ggccagcggc tccgggatga agccaaattg ctgctgagcc ccggtcaggt agtcgcgtgc
     2821 ctcggccgga gccgtagcgg gggtgtgact cacgaaatga gatttgctca tgattatctc
     2881 ccgtcctcgt cctgttcggt ttgttgctta ggcaactata tggaaaaggg gtacgcccgt
     2941 caaccgggcg cacacgcatt tttcgtcacc ttagggcggc ggctgagccg cggatcgtgt
     3001 cggcgcggtg aacgagaatg ccgtcgccct tcgatcactt agagcggtcg tttgctcgga
     3061 tcgcgaggcg agtgcggtcg aagctgaaga tgcccagccg ttctgtcagg gcctttctgg
     3121 ttcggcatgg tgttcagcgg gttaccctcg gcttgggacc ttgtctgagg tcgccgcgcc
     3181 aatagtactc accggttgag catacccatt cagtagagca gcgcctctcc cacgccgcac
     3241 tactcgaagt tagcagtgcc gaagaccagt tggattggcg cttgatgccc ggactgcagc
     3301 gaggtgccaa ttgatactgc gctcgggcgc tcggcagact cgctgtcgcg gttgtccgta
     3361 aactccgcta gttgttttct tgcattcatc tcggctgtcg ctggcgagag ttgacacccg
     3421 aatatgatgg gtggtagttc acagcgtgca gggcgcatac aggctaactc ctttctttgg
     3481 ggcgagtggc tccgcgggat ccggtgttga ttagtgttcg tgctggttcg atgcggcacc
     3541 ctacgccgcg ctcgaagtaa gcggtccacg cgaaggcggg cgctaccttc gggattgacg
     3601 tgggcttgca gtcttcggtg gcggtcttaa gtcgtcgtga gacctgcaag caagcaccgg
     3661 gagtgtcatt gggagttggg tgcgaataaa acaagttggc gcgagtaaac aggagaacat
     3721 tcatggcggg ttcatataag gcgggttcgt ccaaagattc tttgcagtcg agcgcagctc
     3781 cgaagcgttt gcggaatcac tggttgctgg gatcggcgtc cgccgttctg attggcacta
     3841 tagtatcggc gtgctcgggc atggatcaaa ccgcgagtac ctcgggcgcg cactcggaag
     3901 cggtcggcga gcagactgac ttgctctccg acggtagccc cgcggcggga gcaagcgcga
     3961 ctggatcaaa cgcggctgca ggaaacacgg ccgcaggaaa cagagtagag ttttgctatc
     4021 ggggcgagca aatggttcga cccgtcagcg tgtacaatgc caaccgttca cgaggtgcgt
     4081 acctcggggc ctgtgatctc gtggagaacc ggacgtccct gggagacggc tacgttcaga
     4141 cgttcattca tcgcaactcg gacggctcac tgcgtgcgat aggcgtccag atcgaccagg
     4201 tggccatgga taccctgccc atggaacctg tcaacgacgg cgcaacatgc ttcgatcaag
     4261 acggagacgg tgagctcgat ccgcacatgg agtgcagtgg cggtcacgag cgggtactat
     4321 tcttcccgaa ggtcgacggt ctgccgatct cttggatcat gttcaactgg cagtccatgg
     4381 ggcacgcgcc gatgaacgtg ttcgataagg cgcatttcga tctgcactat ttcatccaag
     4441 actacatctc ccggaacttc attcggaccg ggccttgcgg aacgcaggtc aactgtgatg
     4501 acttcgataa ggcgatgcgt gacatcccgg caccgttctt cccggttggg ttcgagaatg
     4561 ttggcggggt cgccggacgc atgggcaacc acctcgtgaa caagtccgag gctccattca
     4621 atggcgagcc tttcacgcag gcgttcgctt atggcgagta cgacgctcat ctatcctttt
     4681 tcgagcccgt ggtgggcacc gattacttga agacgcgccc gaatacctgc ggagcgatca
     4741 agccgccgcc cgcagtcgag ctgagcggcc tgtacccaaa gaaatactgt agtcgcttcc
     4801 gtgcggatcg cggcgactac atcatgaccc tggaagactt cgaatataga gccgccccct
     4861 gatacagagc tcggggcgag cagggctacg tcaggaagtc gttagctagc tggatcgatg
     4921 cgcctgcgcg acggacgcga gtccgccggt tcgcgggagg tggtccagca ctaaccccat
     4981 tgcgggcggg aacgcctgcg atggggagat gctcggagaa gagcgtcgtc caaaccagtg
     5041 ggtccggagg gagcgcaccg cgccgccctc cggacgttcc cgcagtggac cggaagtacg
     5101 gttagagagc tcagctgcga gcgaggtgtc gggaagttct ctgaccctaa ctagtactag
     5161 tccgaacgga tgttctcatg gcggaatcaa gtgaactcta gctctcttca gttctcccgt
     5221 cacccagtga agagtcgcgt gcgcgggttt gctatccggc gttcgcgaat gcggcggagc
     5281 gggagcgcgc ccgtttggat ccccgtgtca gcgttgagct attgcgtcgc atgtatatgg
     5341 cgatgctgcg gactcggatc atcgatgagc aggtgaccgg ttggcaacgg cagggtcgca
     5401 tccgttttca tattggctcc ctgggagaag agtgctgcat catcgcttct gcctgcgcgc
     5461 tgcgcgcgag cgactggatc ttcccgagct atcgcgagtt cggggcgctg ttgatgcgag
     5521 gaatgccgct acgagagtat ctcgggaatt tgttcggcaa cgctcacgac cccgcgaagg
     5581 ggcgtcaaat gccggatcac tacaccggaa agccgttcaa cttcggttgc gtgagctcgc
     5641 cgattgcgac tcaaattcct caagcggtgg ggtttgctta tgcgtcgcgg ctgcggaacg
     5701 aaggcgccgt gattgcggct tacttcgggg acggagctac gagttctggg gagttccacg
     5761 ccgggctgac tttagcgggc gttctgaaga ccccgaacgt ctttctgttg cgcaacaacg
     5821 gctgggcgat tagttgtccc aacgagcgtc agtgtgcggt gcgggctttg tcggacaagg
     5881 cccatggcta cggaattcgg ggcgtcagat gtgacggaaa caacgccgag gagacatatc
     5941 ttacggtgct agaagccgta gagcacgcgg ctcacggccg cggctccact ctcgtggaga
     6001 tggtggcgag tcggcaaggt ggtcattcga cagcagacga ccctcggctt tatcgctctc
     6061 cggaagagga ggcgcgtggc ctcaaggagg acccggttgc gagactgcgt cagcgcctgc
     6121 ggctgctgga agcctggtcc gatgacgagg agcaccagtt gcgtcaggcc gttgccgaag
     6181 agttggtgga agagctgcgc gaggccgagc tagcctcgcc gccaacgctc gagggcctgt
     6241 tcgatgacgt gtacgctcac ctgccggagc acttgaggga gcagcgcgag aaggcgctgc
     6301 ggttcgcata gttcggcgtt cggaggaggg atgactgagc tcaatctggt ccaagcggtc
     6361 aatgccgcat tacgagcgga gatgcggcgc gacgagcgcg tcgtcatatt gggagaagac
     6421 gtgggcaaac tcggcggcgt cttccgggcc acccgtgggc tatacgagga gttcggtcaa
     6481 gcccgggtaa tcgatacgcc cttggccgag aatggaatca tcggcgcagc catcggtatg
     6541 gcgcttcacg ggctcagacc tgtcgccgag atccagtttt ccgatttcat atatccggcg
     6601 ttcgatcaaa tcgtgagtga ggcggcgaag taccggtatc ggtccggggg gcagtttgcc
     6661 gttcccatgg tgatcagggc cccaatcggc ggcggtgtcg cgggcggaat gtaccattcg
     6721 caatccccag aggccctgtt catccacacc cccggcttga aggtggtttg cccctctaca
     6781 ccgtacgatg caaagggcct tttgatcagc gcgatgcgcg atgaagaccc ggtgctgttc
     6841 ttcgagccga agcgcttgta ccgcggccgg ccgatgcccg tgcccggtga agactatacg
     6901 attcccctcg gacgcgcgcg cgtggtgcgc ccgggccgcg acgtcaccct ggtggcgtgg
     6961 ggagccatgg tcgtcgaggc attcgagacc gcggaggagc tcgctccctt gggcgtggac
     7021 tgcgaggtca tcgatctgcg gacgctgtgg ccgttggact tggtgtcgct cgagcaaagc
     7081 accaagaaga cagggcggct cctcgtggtg cacgaggcgc cgctgacttg cggattcgga
     7141 gccgagctcg tggctctgct gagtcagcgt tgcttcgatt acctacgtgc tcctcccgag
     7201 cgcgtgacgg gctgggatat cccctttggc tatgcgcgag aacttgacta cttg
//
LOCUS       DASTKU010001045         6721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_875454, whole genome shotgun sequence.
ACCESSION   DASTKU010001045 DASTKU010000000
VERSION     DASTKU010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875454"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VFQ61_37015"
     CDS             <1..1198
                     /locus_tag="VFQ61_37015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET9960169.1"
                     /translation="GSPEQLMRNPASITGPYLSGQKRLPIPTVRKRPSGAKLRVVGAR
                     AHNLRDVTLEIPLGLITAVTGVSGSGKSSLVVDTLLSAVRAELYGATGWVGPCERLEG
                     LEHIDKVISIDQAPIGRTPRSNSATYTGIFTHLRELYAGLPDARARGYKAGRFSFNVK
                     GGRCEACQGDGVLRVEMHFLPDVYVTCDACGGRRYNRETLEVKYRGLSIADALDLTVD
                     AADELFESIPRIRQKLAALRQVGLGYIKLGQPATTLSGGEAQRVKLATELSRKATGRT
                     LYVLDEPTTGLHFSDIELLTHALFGLRDAGNSVLLIEHNIDLVACADWVVDMGPEGGE
                     HGGLIVAAGTPEEVAESSNSYTGRYLKPALAGETNSVSKGTAKAAEPSSPSAKGDRKS
                     RGRRPN"
     gene            complement(1268..1630)
                     /locus_tag="VFQ61_37020"
     CDS             complement(1268..1630)
                     /locus_tag="VFQ61_37020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HET9960170.1"
                     /translation="MTKVQLKKFKEMLEAKRDEIIKRAQQTLDEDMALDANDLPDEMD
                     LASSEYLQSFQFRLRGREKVFLDKIQKALEKIESGSFGVCDECGEKISVKRLEARPET
                     TLCIRCKEDQERVEKDFT"
     gene            complement(1832..3496)
                     /locus_tag="VFQ61_37025"
     CDS             complement(1832..3496)
                     /locus_tag="VFQ61_37025"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET9960171.1"
                     /translation="MKPQPLNRILTPRVGALPNRKVCGGGALACILLGLMGCGRPSGS
                     TPTSSANSAAPAASALSREDQVELADRLASRASHAQGSDARELHVRAAELRESAYRIQ
                     HRSVDALEAVEQLRAVERDPDAPCQSRLRRILLEAEQSAKPAGAYTEIYALKLDPRTA
                     NCRSEIEQALQALAAFRPPVETLQAIEREHAPRRELGTGSQSSRVLPPQPAVDAANSA
                     PVVITKVERYGAADAARVVVFVSRPSRFEVGEVTTPSGARRVFVDVDSATFSTQSSYE
                     LSGLVEHVRLGARASATRVALDLSAAASHRVFYLPEPYRLVIDVLKADVSQSGLPAKI
                     RRAVLDPGHGGHDPGAIGPSGLREKDVALDIAHRAAPLLARELGISTLLTRDGDQFVP
                     LDERTARANAFRADLFISIHCNASEDPGARGAMVFVLDRSPTSLSHHIAARENATSSE
                     ASVELANSLSKLTDPASLSASTRLAELLGRAAQASFSEKYADVPALPVQRAGFYVLAG
                     AHMPAVLFETSFISNAAGEAHLATPDFRQKLADSIVNAVRAYRAGL"
     gene            3562..5103
                     /locus_tag="VFQ61_37030"
     CDS             3562..5103
                     /locus_tag="VFQ61_37030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960172.1"
                     /translation="MANGSNERPPGGPPNTAGGAPNTEEAERLAELLRPSWELGNGEG
                     PPGAPSPPSEAALQTQQTVQTLQPAQTEQAPAEQTPAEHKAAEQAAAEGAAPEPTRQV
                     SSRRTSKQTLLGLQPPPPPGLEAHAPVSVRPAGVPPATHDSAPPPPPPPPPLGPPGAH
                     EAQPTRDKPTMIGMGRPPEPEPQADQWPPLASPEQSGAQALDSPRGGAAYGTASNEAA
                     SDFVSPPQLVDPELPGTPNERAEPGSVIEAPSQSFSLAKPYVPKDAPNAPPVVVDASV
                     LEAGTSAAQAEAAERRARARRSSTAKTIPGSMRALTVPLPRRAASAVIDEAELAAIKP
                     RRGKVLATLVGVVALAGIAGVLLVNRDKGAGREAVTSATQSALTGPRAEPNSAPSPTS
                     TGAEIAAGPRDPEPSSGSGADEGPSTHQTDEKDSGDKPSNVNDDQAGPNLASSKQEAL
                     PPSTDNVKQGTAPTKEVATITKSTAAKEAASKEGAKKAAAQKRAPSTSQPAKPKPVII
                     RDTPF"
     gene            5448..6641
                     /locus_tag="VFQ61_37035"
     CDS             5448..6641
                     /locus_tag="VFQ61_37035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960173.1"
                     /translation="MVTQKTGVCWTTLRRARGIGRALGLWLAIGSSALHHVPEASAQE
                     DATQAAREAFRQGVTYFDQKQYDKARASFAQAYLLKRHPAVLLNLAQSELRSGKEADA
                     AEHFAQFLRENKEAAEGERQSAEAGLSAAKAAALELTVSVDEDGATVLVDGSPKGTSP
                     LPGPLYLSPGAHTVSAKLEEREASSQLNATAGQTGNLSLRVSKPAPAPKPIAPAPVPS
                     QPAQAEARAEAPAPLAEESVGRPPFFRWVTSSPVGLVGAGLTVLGVGGGIGFAVGSRW
                     AYNRADSDATQIRDATRRDGLARPDGICVDSALQAMTSQPEAYRNACRQYRDDVSQGD
                     TFKTISIASWVVAGAAATGTLAYYFIAAKPAKNAETARDRERSVKILPVLGAAERGLL
                     VVGQF"
BASE COUNT         1307 a         2208 c         2134 g         1072 t
ORIGIN      
        1 aggctcaccc gagcaattga tgcggaaccc agccagcatc acgggcccgt atctctcggg
       61 gcagaagcgg ctccccatcc ctacggtgcg caagcgcccc tcgggcgcca agctgcgggt
      121 ggtcggcgcg cgcgcccata atctgcgcga cgtcacgctc gagatcccgc tcggcttgat
      181 cacggccgtg accggagtga gcggctccgg aaaaagcagc ctcgtggtgg ataccctgct
      241 gagcgccgtg cgcgccgagc tgtacggagc gaccggctgg gtcggcccgt gcgaacgtct
      301 cgagggcttg gagcacatcg ataaggtcat cagtatcgac caggccccga tcgggcgtac
      361 cccgcgctcc aattccgcga cctacaccgg catcttcacg catctgagag agctctatgc
      421 cgggctgccc gatgcgcgcg cgcgtggcta caaggcaggg cgcttctcct tcaacgtgaa
      481 gggtggtcgt tgcgaggcgt gccagggcga cggggtgttg cgcgtcgaaa tgcacttcct
      541 cccggacgta tacgtgacat gcgacgcttg cggtgggcgc cgctacaacc gcgagacctt
      601 ggaggtaaag taccgcggat tatcgatcgc cgacgcgctc gatttgaccg tcgatgccgc
      661 cgacgaactg ttcgagtcga tccccaggat tcgccaaaaa ctcgcggcac ttcgccaggt
      721 cggcctgggt tacataaagc taggtcaacc tgccacgacc ctctcgggcg gtgaggccca
      781 acgagtcaaa ctggcgacgg agctctcccg aaaggcaacg gggcggacgc tctatgtgtt
      841 ggacgaaccc accaccgggc tccatttctc cgacatcgag ctgctgaccc acgcgctctt
      901 cggtctgcgc gacgccggca attccgttct actcatcgag cacaacatcg atctcgtagc
      961 ttgcgccgat tgggtcgtgg acatgggccc ggagggcggc gagcacggcg gactcatcgt
     1021 cgcggcaggc acacctgaag aggtcgcgga gagctcgaac agctacacag gtcgttactt
     1081 gaaacccgcg ctggcgggtg aaacgaactc cgtgagcaag gggaccgcca aggccgcaga
     1141 gccttcgtcc ccatcagcta aaggtgaccg caagagccgc gggcgccgcc cgaactgact
     1201 cgccacgcga ccgagatgcg atccggcggc tcgcgctctg aaaacgcgag ccgctttctt
     1261 gcaagcttca ggtgaagtct ttctccacgc gctcttgatc ttctttgcag cgaatacaaa
     1321 gcgtcgtctc cgggcgcgcc tcgaggcgtt tgacggagat cttttcgccg cattcgtcac
     1381 agaccccgaa gctgccgctc tcgatcttct cgagcgcctt ctggatcttg tccaagaaca
     1441 ccttctcacg accgcgcagc cggaactgaa acgactgcaa atattcgctg gatgcgagat
     1501 ccatctcgtc agggagatcg ttcgcgtcga gcgccatatc ctcatcgagg gtctgctggg
     1561 cacgcttgat gatctcgtcc cgcttggcct cgagcatttc tttgaacttc ttgagctgaa
     1621 ctttcgtcat cttcggctcg ggaactaaac aaaaaccggc caaaccggcg aattcagggt
     1681 ggcccgccaa cataagaata ggaggcgctc gagtcaatgg gagacatggc aactcctgga
     1741 ttcatgtgag ttccgcgatg cggccaaacg accgaattac aggaatgcgc tcaacttcgc
     1801 caagctcggc tccgcccacg ccaaagggat tctataaccc tgcacggtag gcgcgcaccg
     1861 cgttgacgat cgagtcagcg agcttctgcc gaaagtcggg cgtcgcgaga tgggcctcgc
     1921 ccgcggcgtt cgagatgaat gaagtctcga acagcacggc cggcatgtgt gctcccgcca
     1981 atacgtagaa cccggcgcgt tgcacgggta acgctgggac atccgcgtat ttttcggaaa
     2041 aggacgcctg agctgcacgg cccagaagct cggccagacg cgtcgacgca ctcagactgg
     2101 ctggatcggt cagtttggag aggctgttcg ccagctcgac cgaagcttcg gaggaggtgg
     2161 cattttcgcg cgcggcgatg tgatgcgaga ggctcgtggg gctgcgatcc agcacgaaaa
     2221 ccattgcccc ccgagccccc ggatcctcac tcgcgttgca gtgaatcgag atgaacaggt
     2281 ccgcgcgaaa ggcgttcgca cgtgcggtgc gctcatccag cggcacgaac tgatctccgt
     2341 cgcgagtcag taacgtggag atgccaagtt cacgagccag caacggcgcc gcgcggtgtg
     2401 cgatgtcgag ggcgacgtcc ttctcccgta aaccagaggg cccgatggcc ccggggtcgt
     2461 gaccgccgtg gcccgggtcc agcacggccc gccgaatttt cgccggcaac ccgctctgcg
     2521 aaacgtcggc tttcagcacg tcgatcacca aacgatacgg ctctggcaag tagaacacgc
     2581 gatgactcgc ggccgcactc aaatcgagag ccacgcgcgt ggcgctggct cgagcgccga
     2641 gccgtacgtg ctcgacgaga cccgaaagtt cgtagctgga ttgagtggaa aacgtggctg
     2701 agtccacgtc cacgaaaacg cgccgagccc ccgatggagt cgtgacttca cccacctcga
     2761 agcgactcgg gcggctcacg aacacgacca cgcgcgcggc atcggcggcg ccgtaacgct
     2821 ccactttcgt gatcaccact ggcgcgctat tcgctgcatc gacggcgggc tgcggcggca
     2881 acacgcggct cgattgagag cccgttccga gttctcggcg gggcgcatgc tcgcgttcga
     2941 tagcttgcag tgtctcgacg ggaggtcgga aggcagccaa cgcttggagc gcctgctcga
     3001 tctcggagcg gcagtttgcc gtccgcggat cgagtttcag agcataaatt tccgtgtagg
     3061 ctcctgctgg cttggcggat tgctcagcct cgaggaggat gcgacgaaga cgcgactgac
     3121 aaggagcatc cgggtcgcgc tccacggcac gcaattgttc cacggcttcg agtgcatcca
     3181 cgctgcgatg ctgaatccgg taagcgcttt cacgcaattc cgccgcgcgg acgtgtagct
     3241 cgcgggcatc actgccttgc gcgtgggaag cgcgcgacgc cagccgatcg gccaattcga
     3301 cctgatcttc gcgggagaga gcagatgcgg ccggtgcggc ggaattcgcg ctcgacgtcg
     3361 gtgtggaccc ggatggcctc ccgcagccca ttagccccag gagaatgcac gccagagcgc
     3421 cgccgccgca gaccttgcgg ttggggagcg ctcccactcg gggcgtaagg atccgattca
     3481 ggggttgtgg cttcactctc gaatcgggac agcgcgaatt gcgcggtcct tcatcacggt
     3541 gctagcctgc ttccctgcgg aatggcaaac ggctcgaacg agcggcctcc aggaggtccg
     3601 ccgaatacag caggcggagc gccgaatacg gaagaggctg aacggcttgc agagcttctg
     3661 cggcccagct gggaactcgg caatggtgaa ggccctcctg gcgcgccttc gcctccatcc
     3721 gaggccgcgc ttcagactca gcagactgtg cagactctgc agcctgctca gaccgagcaa
     3781 gcccccgcag aacagacgcc cgcagagcat aaagcggcgg agcaggcggc agccgagggc
     3841 gccgcacccg agcccaccag acaagtctcg agtcgccgca cctcgaagca gacgctgcta
     3901 ggtcttcagc ctccgccacc tcctgggctc gaagctcacg cgccagtcag cgtgcgcccg
     3961 gctggcgtgc cccccgccac tcacgatagc gctccccctc cgccacctcc cccgcccccc
     4021 ttggggccgc cgggagcgca cgaagcgcag ccgacgcgcg acaagccgac catgatcggt
     4081 atggggagac ccccggaacc ggagcctcag gcggatcaat ggccgcccct cgcatcgccc
     4141 gagcagagcg gcgcgcaagc gctggatagc ccgcgcggag gcgcggccta cggcacggct
     4201 tccaacgagg cagcgagcga cttcgtcagt cccccgcaac tcgtggatcc agaactgccc
     4261 ggcactccga acgaaagagc cgagcccgga agcgtcatcg aagcgccatc tcagtctttc
     4321 agtctggcga agccctacgt ccccaaggac gcacccaatg ccccgcccgt cgtggtggac
     4381 gcttccgtac tggaggccgg cacatcggcc gctcaagccg aggcggccga gcgccgcgcc
     4441 cgcgctcggc ggagctccac ggccaaaaca atcccgggca gcatgcgtgc gctcacggtt
     4501 ccgctcccgc ggcgagccgc gtcagcggtc atcgatgagg cggaattagc ggcgatcaag
     4561 cccagacggg gcaaggtgct cgccaccctg gtcggcgttg tcgctctcgc tggcattgca
     4621 ggcgtattgc tcgtgaatcg cgacaaggga gcagggcgcg aagcagtgac gagcgcgacg
     4681 cagagcgcgc tgactgggcc ccgcgccgag ccaaactcag ccccgagccc cacatcaacc
     4741 ggagcggaga tagcggcggg accacgcgac ccggaaccca gctcgggttc gggtgcggac
     4801 gaaggaccct cgacccatca aacggatgag aaggacagcg gggacaagcc gtcgaacgtc
     4861 aacgatgatc aggcgggtcc gaacctggca agctccaaac aagaggctct gccacccagc
     4921 actgacaacg ttaagcaagg cacggcaccg acgaaagagg tggccacgat aaccaagagc
     4981 accgcggcta aagaggccgc cagcaaagaa ggcgccaaaa aagctgctgc tcaaaaacga
     5041 gccccctcga cctcgcaacc ggctaagccg aagccggtca ttatccggga cacacctttc
     5101 tagttgcgag cgccgcgtgc aacggtcagg cgtgccgaag ggagagcgag ccaacgcttg
     5161 ctgaacccgg tatgaaagct gaggacttgt tctactcagc cccgctccgc caactttcgc
     5221 gcgctctcgg tcggcaccgc aaccgattcc agaacgcaag ctatccgctt ggctcgaagt
     5281 tttttgggaa tcctgcggga acccttttgt ggaagcgagg ggaaagcaag caaggagcga
     5341 ttggcttttt gcgcgcgtcc gcctaaagaa catgcgttgg ttccgttccc cgcatccgca
     5401 cgcaacgcgc taactacgga aggtctgcga cccgcagaag gcttagcatg gtgacgcaaa
     5461 agaccggtgt gtgttggacc acgctcagac gcgctcgagg catcggccgc gcactggggc
     5521 tctggctcgc gatcgggagc agcgcgctcc atcacgttcc cgaggcgtcc gcgcaagagg
     5581 atgcgaccca agccgcgcgc gaagcgttcc gccagggcgt cacgtatttc gatcagaagc
     5641 agtatgacaa ggcgcgtgcc tcctttgcac aagcttatct gctgaagcgc cacccggcgg
     5701 tgctgctcaa cctcgctcaa agcgagctcc gctcgggcaa ggaggccgac gcggccgagc
     5761 atttcgctca gttcttgcga gagaacaagg aggccgcaga aggcgagcgt cagagcgcgg
     5821 aggcgggctt gagcgccgcg aaggccgccg cgctcgagct cacggtgagc gtggacgaag
     5881 acggcgcgac ggtgctggtg gatggatccc caaagggcac ctcccctctg ccgggaccat
     5941 tgtacttgtc gccgggggca cacactgttt ccgcgaagct cgaggagcga gaggcttcga
     6001 gccagctcaa tgcgactgcc gggcagaccg ggaacctgag cttgcgcgtc tccaagcccg
     6061 cacccgcgcc aaaacccatc gccccagcgc ccgtgccgag ccagcccgcc caggccgaag
     6121 cgcgcgccga agcccctgcg ccactcgcag aagagagcgt aggccgacct ccgttcttcc
     6181 gatgggtcac gagctcaccc gttggcctgg taggcgctgg gctcaccgtg ctcggagtgg
     6241 gcggcgggat tggtttcgca gtgggctctc gttgggcgta caaccgcgcg gactcggatg
     6301 cgactcagat ccgggatgca acccgcaggg atgggctagc tcgccccgat ggcatctgcg
     6361 tcgactcggc attgcaggcg atgaccagcc agccagaggc ctaccggaac gcgtgtcgtc
     6421 agtatcgcga cgatgtctcg cagggcgaca cattcaagac catcagcatc gcgagctggg
     6481 tcgtcgcggg cgcggccgca acgggaaccc tggcctatta tttcatcgcc gccaagccgg
     6541 cgaagaacgc cgaaaccgcc cgtgatcgcg agcggtcggt gaagatcttg ccggtgcttg
     6601 gcgccgccga gcgcggcttg ctcgtagtcg ggcaattctg agctcacgcg gagcgttgct
     6661 cacgactcag ccatgacctg aacgtgctgc gcggcacgcc ggccgcgcga gcggcggcgc
     6721 t
//
LOCUS       DASTKU010001046         3261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1547760, whole genome shotgun sequence.
ACCESSION   DASTKU010001046 DASTKU010000000
VERSION     DASTKU010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547760"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /gene="mutM"
                     /locus_tag="VFQ61_37040"
     CDS             <1..1008
                     /gene="mutM"
                     /locus_tag="VFQ61_37040"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HET9960174.1"
                     /translation="ARHPKGAPTHRSPRVKRRKNCDQRHVSFARVRTTSVCDYNSASR
                     MPELPEVEVTRRQLLPFFVDRTIEDIATTKPSYFFITRPKELARRLIGHRICALDRVG
                     KYLLLRLDDDSTVLLHLGMTGQLFAAGASNPRLLSATQKRALGPRQQLDFTPDVHTHL
                     VFAFRDPGPKVFFRDVRKFGKVAWLPAGKQHSRLEKLGKDALGASGADLFEASQRRKV
                     EIKSLLLDQGVIAGIGNIYADEALFLAGVRVTRPAHRVTRAECEKIVRAAQQVMLRSI
                     ETGGSSISDYVRPDGSDGGYQNERRVYARTGEACGVCGQPIQRIVIAQRSSHFCKHCQ
                     K"
     gene            complement(1141..2034)
                     /locus_tag="VFQ61_37045"
     CDS             complement(1141..2034)
                     /locus_tag="VFQ61_37045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET9960175.1"
                     /translation="MPVEAVMLWNEPNNASHWDFEQDKDWSCFAKMIKHAAQAVRAEN
                     PNLKIVLGGISPIDPEFLRNMERQGVLDAVDVVAVHGFPLDWNHWTIHEWPQRLEDVR
                     AVTKLPIWVTEVGVSTFGAEEVQQFGLKRTAQLLVGKVPRVHWYSLFDLPRAWPATTR
                     HREAEGSSYYRHFYMGLIREDGSPKPALNDFREFTPDLGLCQWIHFEDPRLDLAANWL
                     RELGVRYLRTGLSWADSYRPNALSWFDRQMKTLENFEVTLTYCFTPEHCGLKPHHTSP
                     PQHPEQYADFCADMTRRYARG"
     gene            complement(2114..>3261)
                     /locus_tag="VFQ61_37050"
     CDS             complement(2114..>3261)
                     /locus_tag="VFQ61_37050"
                     /inference="COORDINATES: protein motif:HMM:NF025244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4202 family protein"
                     /protein_id="HET9960176.1"
                     /translation="RHTCAHRVDELLSICRELGLNTQVASGALEPESTRAPVEVARAN
                     LRVALDLGIQVRPAECAVGLRLVLPSDPRRQARISRVLAEFPAIRVEFIPFDAPVDPA
                     LTLEVFEWARRDCDLAECDRIAAHAADSSAWLSLRGADLPEDVLFQVLTRYQRLLTRT
                     DVACCDPLFARVLERHRALHDLSKPLVRADFDHAIDVWLWLLRIEPGASRALQWAALF
                     HDIERLESEADFRIEQHAPDYQAFKDAHAEQGARMTRAVLTAAGVTPEEADQVAQWIV
                     WHERPAHEDRSYDSSALADADALSFFSLNSPGFMNYYGLEHTQKKIAYSLSRMSPRAK
                     KLLTRVKLRADVQDALLRTYPGGAAAKAAELAANFESSFETEPTLNQ"
BASE COUNT          605 a         1086 c          982 g          588 t
ORIGIN      
        1 gcgcgccacc cgaaaggtgc tccgacgcac cgctcaccgc gcgtgaaacg ccggaaaaat
       61 tgcgatcaac gccacgtgtc gtttgctcgc gttcgaacca cctccgtttg cgattacaac
      121 tcagcttcgc gcatgcccga gctccccgaa gtcgaagtca cccgccgcca gcttctcccg
      181 ttcttcgtcg atcgtacgat tgaagacatc gcgaccacca agccgagtta cttcttcatc
      241 acgcgaccca aggaactcgc gcggcgcttg attggtcatc ggatctgcgc gctcgaccgc
      301 gtcggtaaat acctgctgct gcgcctcgac gacgactcga ccgtgctcct ccacctcggc
      361 atgacaggtc agctattcgc cgccggcgcc agcaacccgc ggttgttgtc ggcgactcaa
      421 aagcgagccc tggggccacg ccagcagctc gacttcaccc ccgacgtgca tacccacctc
      481 gtgttcgcct tccgagaccc tggccccaaa gtcttcttcc gcgacgtccg caaattcggc
      541 aaagttgcct ggctgccggc gggcaaacag cactcacgcc tagagaagct cggcaaagac
      601 gcgctcggcg ccagcggcgc ggatctcttc gaagccagcc agcgccgcaa agtcgagatc
      661 aagagcttgc tcctcgatca aggcgtgatc gccggcattg gcaacatcta cgccgacgag
      721 gccctgttcc ttgccggtgt gcgcgtcacg cggcccgccc atcgcgtcac tcgcgctgaa
      781 tgcgaaaaga tcgtccgggc cgcgcaacaa gtcatgctgc gttccatcga gaccgggggc
      841 tccagcatca gcgactacgt gcgccccgac ggcagtgacg gtggctatca gaatgaacgc
      901 cgcgtctacg cccgcaccgg agaagcctgc ggcgtgtgcg gccagcccat ccagcgcatc
      961 gtcatcgccc agcgctcctc acatttctgc aaacactgcc agaagtagag gtcaggcttg
     1021 cggctggcca cttcgtaaag agcgcgaccg tagtcgcgcc ccgtcgccag cgcagccgcg
     1081 agcccaccga accgccctcc attaccgcgc cggccccctc tgcaatcgcg ccaagtcgtt
     1141 tcaaccccgc gcatagcgtc gggtcatgtc ggcgcagaag tcggcgtatt gttccggatg
     1201 ttggggcggg ctcgtgtgat gcggtttgag cccgcagtgc tcgggcgtaa agcaatacgt
     1261 gagggtcacc tcgaagttct cgagcgtctt catttgtcga tcgaaccagc tcagcgcgtt
     1321 cggtcgatag ctgtcggccc aagacaggcc ggtgcgcagg tagcgcacgc cgagctcacg
     1381 cagccagttg gcggccaaat ccaagcgcgg atcctcgaag tgaatccatt ggcagagccc
     1441 gaggtcggga gtgaactcac gaaagtcatt cagcgctggt ttcgggcttc cgtcctcgcg
     1501 gatcaagccc atgtagaagt gacgatagta cgaggagccc tccgcctcgc ggtggcgggt
     1561 cgtagccggc catgcccgcg gcaggtcgaa caagctgtac caatgcacgc gcggtacctt
     1621 ccctaccagc aactgcgcag tgcgcttcaa cccgaattgc tgcacttcct cggctccgaa
     1681 cgtggaaacc ccgacctcgg tcacccaaat cgggagcttg gtgacggccc gcacgtcctc
     1741 taagcgctgc ggccactcgt ggatcgtcca gtggttccaa tcgagaggga aaccgtgcac
     1801 cgcaaccacg tccaccgcgt cgagcacccc ttggcgctcc atgttgcgga gaaactccgg
     1861 atcgatgggc gatatgccgc caaggacaat cttgaggttc gggttctccg cgcgcacggc
     1921 ctgcgccgcg tgcttgatca tcttcgcgaa gcaactccag tccttatcct gttcgaaatc
     1981 ccagtgcgaa gcattgttgg gttcgttcca gagcatgacg gcttcgacgg gcattcaatc
     2041 acctttgctt gggtaagagt ggggtcagcg ggcccggtcg ggctcgtgtt tggagaagtt
     2101 gattcgcttc gaatcactga ttcaaagtag gttcggtctc gaagctcgac tcgaagttcg
     2161 cggcgagctc agcggccttg gcggccgcgc ctccgggata ggtgcgtaac aaggcatcct
     2221 gcacgtcggc gcgcagcttg acgcgcgtca gtagcttctt cgctcgtggg ctcatgcgcg
     2281 acagcgaata agcaatcttc ttctgcgtgt gctcgagacc gtagtagttc atgaaaccgg
     2341 ggctgttcaa agagaagaac gacagcgcgt ccgcgtccgc cagcgcgctc gagtcgtagc
     2401 tgcggtcttc gtgcgcgggg cgctcgtgcc agacgatcca ttgggctact tggtccgctt
     2461 cctccggcgt gaccccggct gcggtgagca ccgcgcgtgt catgcgcgcg ccttgttcgg
     2521 cgtgcgcatc cttgaaagct tggtaatcgg gggcgtgctg ttcgatgcga aagtcagctt
     2581 cgctctcgag ccgctcgatg tcatggaaca gcgccgccca ttgcagagcg cggctcgccc
     2641 cgggctcgat gcgaagcaac catagccaca catcaatcgc gtgatcgaaa tccgcacgca
     2701 ccagcggctt gctgaggtcg tgaagcgccc gatgccgctc cagtacgcgg gcaaagagcg
     2761 ggtcacagca ggccacgtcg gttcgggtca gcagcctctg atagcgggtc aagacctgaa
     2821 acaatacgtc ttccggtagg tctgccccgc gcaaggagag ccacgccgag ctgtccgcgg
     2881 cgtgggccgc gatgcggtcg cactcggcca agtcacaatc acgtcgcgcc cactcgaaga
     2941 cctccaaggt caaagccgga tccaccggtg cgtcaaaggg aatgaactcc acccgaatcg
     3001 ccggaaattc ggcgagcacg cgggagatcc gggcctgtcg gcgcgggtcg ctcggtagga
     3061 ccagccgtaa ccctaccgcg cactccgccg ggcgcacttg gatgccgagg tcgagagcaa
     3121 cgcgcaggtt cgcgcgcgcc acctcgaccg gagctcgggt cgactccggc tcgagcgcgc
     3181 ccgacgccac ttgcgtattc agcccgagct cgcggcagat cgagagcagc tcatctaccc
     3241 ggtgcgcgca ggtgtgtcgt g
//
LOCUS       DASTKU010001047         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1548001, whole genome shotgun sequence.
ACCESSION   DASTKU010001047 DASTKU010000000
VERSION     DASTKU010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548001"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFQ61_37055"
     CDS             complement(<1..377)
                     /locus_tag="VFQ61_37055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960177.1"
                     /translation="MSISSCSPIELTPAQPVDFESLCTLRLDAMRESLERIGRFDVQR
                     ARDRFRATFTPEATRNIVSNGVRVGFVGARFVDDAIALDHLYVHPSSAATRGQPDFVT
                     GDAFSKIKDSRGNRWSIFYLCAT"
     gene            605..>2126
                     /locus_tag="VFQ61_37060"
     CDS             605..>2126
                     /locus_tag="VFQ61_37060"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HET9960178.1"
                     /translation="MSSWAQRNHLTLRALLGLGLGLASSAPRSAEACGGFFCNQAQPV
                     NQAAEKIIFADDGEGNITAVIEITYAGPAQAFSWLLPIPTAATAPTLELSSQTALDRL
                     QQRTNPSYQLTRRVEGECDTDFSLGFAGSDSASAGRIAEAPGDVVQVSGGTVGPFDWT
                     VIRVEPGVPEPAAAAVVWLRQNDYDVPEGASALLNPYLEEQQSLLALRLTKGATTGAI
                     RPIVVSYPAAKPSIPVRLTAIAAEDDMGVMTWLLGNGRGVPENYYSLQLNEARINWFN
                     PASNYESVVNEAADDAGGQGFVTEFAGPSRLLQSAVWNEGEEQAWLAVRRELDAATEP
                     QALSLVWARFGALDGFWEAVGGSLQLPAGVGLEDLKACPSCNGWVEQTTLTPNGVAAK
                     LESGVIDPLRRVQGLIDAHPYVTRVYTTLSATEMTVDPVFTFNPDLEDVSNIHTASQV
                     IECTSAVKEQEASWRIELPQGDVVRGHAATTGLWPEALTSLPANLEIRRLSSSGDGK"
BASE COUNT          411 a          636 c          688 g          391 t
ORIGIN      
        1 aaagtcgcgc acaggtaaaa gatcgaccat cgattcccgc gagagtcctt gattttggag
       61 aaggcatcgc ccgtgacgaa atcgggctga cccctcgtcg ctgcggagga cgggtgcacg
      121 tagagatgat cgagggcgat cgcgtcgtcg acgaatcgcg cgccgacgaa gccaactcgt
      181 actccgttcg atacgatgtt cctggtcgcc tcgggcgtga acgtagccct gaaccggtca
      241 cgcgcgcgct ggacgtcgaa acgcccgatg cgttcgaggc tctctcgcat ggcgtcgagc
      301 ctgagcgtgc acagcgattc gaagtccacg ggctgtgcgg gagtgagctc aatcggggag
      361 cagctcgaga tcgacatgcg ctcagcatcc gcgcaggggc gcctcggctg gaagcaaaag
      421 cctttctctc gtaccaggcc gtatctaaca gattcgcacg cgcgggctga gtttcgacac
      481 atgttatgtg cactcgagtg ctagcgtcga gggcagccat ttggcacact cacccaggtt
      541 atcgtgagtg cacggagcga aggagtgtcc tgaaaccacc gttgcagtca ctcggaggtt
      601 cgagatgtcg agttgggcac agcgcaatca cttaaccctt cgcgcgcttt tgggcttggg
      661 tctggggctc gcgtcgagcg cgccgcgctc tgccgaagct tgcggcggct tcttctgcaa
      721 tcaggcgcag ccggtcaatc aggcagcgga gaagatcatc ttcgcggacg atggcgaagg
      781 caacatcacc gcagtgatag agatcactta cgcgggcccg gcgcaagcct tctcttggct
      841 attaccgatt cccacggcgg cgacagcgcc gaccctcgag ctctcctcac agaccgcgct
      901 cgaccgtttg cagcagagga cgaacccgag ttatcaactc acgcgccgcg tggaagggga
      961 gtgcgacacg gatttcagtc ttgggttcgc tggatcggac agtgcctcag ccggtaggat
     1021 tgcagaagct cccggcgacg tggtgcaggt gagcgggggc accgtaggac ctttcgactg
     1081 gacggtgatc cgcgtggagc ccggtgtacc agagccggca gcagcggcgg tcgtctggct
     1141 gcggcagaat gactacgacg tacccgaggg ggcgtctgcg ttgctgaacc cgtacctcga
     1201 ggagcagcaa tccttgctgg cgttgcgctt gaccaagggc gcgaccaccg gcgccataag
     1261 gcccatcgtg gtgagctacc ctgccgccaa accttcgatt cctgtgaggc ttacggccat
     1321 cgctgcggag gacgacatgg gggtaatgac ttggcttctg ggcaacggac gtggagttcc
     1381 ggaaaactat tactcgctac aactgaacga agcgcgtatc aactggttca atccagcctc
     1441 gaactacgag tctgtcgtca acgaagccgc ggatgacgcc ggtgggcagg gcttcgttac
     1501 ggagtttgcg ggacctagcc gcctcttgca gtccgcggtt tggaacgagg gcgaggaaca
     1561 agcgtggttg gcggtgcgcc gcgaactcga cgccgcaacg gaaccccaag ccctctcgct
     1621 ggtttgggcc cgcttcggtg ctttggacgg gttttgggaa gccgtcggcg gcagcctcca
     1681 gttacccgcg ggggttgggc tcgaggatct caaagcgtgt ccctcttgca atggctgggt
     1741 cgaacaaacc acgctgacgc caaacggcgt cgcggccaag ttggagagcg gcgtcatcga
     1801 tccgctgcgc agggtccaag gtttgatcga cgctcacccc tacgtgaccc gtgtttacac
     1861 cacgctctcg gccaccgaga tgaccgtaga tccggtgttc accttcaatc ccgacctcga
     1921 ggacgtgagc aacatccaca ccgcgtcgca ggtcatcgaa tgcacttctg cagtcaaaga
     1981 gcaggaggca tcctggcgga tcgagttgcc ccagggcgac gtcgtacgcg gacacgccgc
     2041 aacgacaggg ctctggcccg aagctttgac gtcgctgccg gccaacctcg agatccggcg
     2101 gttgagcagc tcgggggacg gcaagg
//
LOCUS       DASTKU010001048        21247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_428823, whole genome shotgun sequence.
ACCESSION   DASTKU010001048 DASTKU010000000
VERSION     DASTKU010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 21247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21247
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428823"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFQ61_37065"
     CDS             complement(<1..495)
                     /locus_tag="VFQ61_37065"
                     /inference="COORDINATES: protein motif:HMM:NF018210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960179.1"
                     /translation="MYGLNLKLVERFFVISKPYWFSEEKWRARGLFALLLVLLVLETQ
                     FNVRFNEQSGEFTSALAARDAPRFFRAMRTFLALLVFAVPIFAFYYYVRDKLAIGWRR
                     WLSYRFLGKYFDNRAYYHLLGSPEIDNPDQRIADDIAAFTQKSLSLSMMFASSWFQVL
                     AFGRV"
     gene            611..2953
                     /locus_tag="VFQ61_37070"
     CDS             611..2953
                     /locus_tag="VFQ61_37070"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HET9960180.1"
                     /translation="MDLSALALRGLLGSRKPIHTGRFRVACQRELSVRRDGFGVAYID
                     AQNEADAWFGLGFCHAQDRAGQLEVTLRLTRGTLSELVGREGLHVDRAVRLIGFSRAA
                     ARQLETFDPQTRAQLEAYTAGINAAFACGAAPRSHEHVLLRSPMTPWQPADCIALGLL
                     TCCFLPSNWDLELARLLIWCHDGPDAVAKVDPNFRRHPSNGQTPSELFLAEDLSALQR
                     FLGPLGGSNAWAVAPHKTAHGRALLANDPHLPANLPNLGYLARVVCPSFEVTGVSLIG
                     IPAFITGHNGYAAWGTTAAHVDNVDLFLEELGPGGKTVREGQDFVPIQVETQSIVVKG
                     EAPLELEVASTARGPIVARSSSPEQGFFRHVPGVGGANALSFAATWLEPRPTRALLGL
                     HHVRSFEEFRETCAAATGCSYSLVYADPESIGWLMASEVPRRKSGYGSLPLPGWSPHV
                     GWIKPAIPAQRLPWSRNPESGFVSCANDDPVREHAEDVFLGHDFLDHQRKTRIGEVLR
                     EGSEFTIESMQRLQMDVLSIPFRELRSSLIELRPKQALAQRAVELLAAWDGQATSDSV
                     AATVFELFLSELGQRVCAQAAPRAWQFALGQSVSHLIPGTTFNYRRGSFLVRWIRDSL
                     TGTSRESFAVELGDALHDVMLTLRTRFGEREADWVWGAVRPLQLRHRLGTVQPLAAWF
                     NRGPYRGLGNSTTINMASVDIRMPLRTPTITAHLRAVYEIGNFEASRFVLLGGQSGNP
                     LSAHYTDLVPFWQRGEGVPIHCAEAAIDRHAVAELRLVPA"
     gene            complement(2968..4719)
                     /locus_tag="VFQ61_37075"
     CDS             complement(2968..4719)
                     /locus_tag="VFQ61_37075"
                     /inference="COORDINATES: protein motif:HMM:NF013438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET9960181.1"
                     /translation="MSTRFSLFIGAVLCACVSPLPPRTDDSSGGTANPGGGSGASLEG
                     GSGGAPNGNAGQSGDARSSGGRASAGGTFNPQGGTRTTTTEATSGGARGGTSGEDAGQ
                     GGDTEASGGRASGGATAGGTSTGGMSSGATAAGGATGGTAGQNGLGGDNFGGTNASGG
                     DAGSLSEGGSTGSIEILPRATANLFVTLLDEDPAQVEAKVSTAVNRFFGIGTGEKNEL
                     IVNTGYRCYFELPQNPELAFIWAPDTNDIRSEGMSYGMMIAVQMNLQTPFDKLWRFAK
                     TKMQYTADTDPPAWRYYFRWQSIQETGINALDPALWKIDYNPTTTPAPDGEEYFAAAL
                     YLAHKRWGSAGAINYLQEANALASAMLHNASTNDDRHPIIHPTQNMVVFVPYGASNGF
                     TDPSYHLPAFYELFAERGPQSDRARWKNIAQISRTFLAASAHPSTGLHPDYATFAGAP
                     RTAATGDGHDRFKYDAWRVPLNMALDYAWFSADPGLKAQLEKYQAFFTGQLVQGNVSN
                     SLFAVDGSAGTGGGSTALTATLAAASLGSAAPNRAQFVRNLWLVDQQSGKYRYYQQGV
                     YLLGLLATAGKFRTTWE"
     gene            complement(4841..6547)
                     /locus_tag="VFQ61_37080"
     CDS             complement(4841..6547)
                     /locus_tag="VFQ61_37080"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET9960182.1"
                     /translation="MRTATYFTSLALLASCTSSPESHPQHTSAAAVQRAVDASTTTVT
                     VDFGTREQRISGFGTSSAWTTPTLTDEQADFFFSEERGLGISLLRLRIAPDGTTLETR
                     TALRAQERGVRVWAAPWSPPGEWKTNGTDNHGGSLLPEYYQAWAERLAAFTREMKQQG
                     VELIALSAQNEPNWTAEWETCNWTPAELVTFIRDHLGPALQDASPNTLLMAPESANWT
                     GLRSYTDALLADEQAREFLGIVATHAYGGFPFDYPKIREYQKEFWETEISYSDSRGIS
                     AALATARMIHDHLSIARVNAWNYWWMNSDNETSLLKNGEVLPQAYGLGHFSKFVRPGF
                     YKVQAEPRGPSTGIGVSAFADPDGERFAIVTFNDNDYPVTQRFAFQSLPSGSLRPWLT
                     REGIQLEAQPVVTATNSFEYTLPASSIITFVGTDDPPDLPVGEGGAGGTGAGAGADAG
                     GSAGQDTEPTAGASAVDPETGGSSATPNGGKTTTSSGGRAASGGSSAMGGAAGGAMST
                     DPTPSLGGSVSGRAPEYPVIPGCYCRAASRTQAYSGASWLLALGSVGAFRLRRRPPRN
                     RG"
     gene            complement(6628..7518)
                     /locus_tag="VFQ61_37085"
     CDS             complement(6628..7518)
                     /locus_tag="VFQ61_37085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960183.1"
                     /translation="MMYPNKWAFAAMITTGTAALLGSPSLARAQESSQQCASAYEEAQ
                     RQRAKGDFREASRAAQTCSQAECNALLVQECIKLYEQIQADTPSMVFSVRNGNGEELV
                     NVKVTVDGQLVTDHLDGRPIEMNPGLHTFYFESEGLPPLETKHTARVGDRNRLLEIVL
                     GDVRPKTADKPEGSAAAAGSALPPAAPPPNSVPIPSIVLGGVGLLGLGTFAYFRVTGT
                     SDYNELGKTCSPRCDPAETDKIHTKFLVSYVGLGVGAAGLGGAALWYVLTRNREESPE
                     PSVAVVPTHDGIRAQWQARF"
     gene            complement(7593..9023)
                     /locus_tag="VFQ61_37090"
     CDS             complement(7593..9023)
                     /locus_tag="VFQ61_37090"
                     /inference="COORDINATES: protein motif:HMM:NF015390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HET9960184.1"
                     /translation="MRSFRELRFSLAALTSLGIVAACEPDLEPLSAQFGSGGRAQTGG
                     SGGTTASGGASQGGTTGDTGGTTTGQGGATGTEVPDTCSDLRRNADESDVDCGGTSLC
                     DRCEINGRCTRHGDCATELCVKGRCAEPSCEDLILNQNETGTDCGGVCAPASLCPDET
                     ACKVNGDCQSDYCKDEVCTDHCSSKKIDADETDKDCGGTTCDPCPNDSACLKITDCAS
                     GVCKNNICVPPSCQDDVKNQDESDIDCGGVCSPDQACPNGKKCVKGTDCESLVCSGSP
                     ARCVPDIVIAEGDMIDNFEDGDNIIPRISGRAGNWYRFDDQTGTSLFEPKFPDRGTSK
                     FALHYAGQDFQNYSGIGVDLNNTGGSESTKGLWDGSGHTGITFWGRGDKPATVKVQMA
                     DKNTSRAGGICTATNTCDKHWYKIVTISGDWKRYTLSFADFVLEPGNMPEPAELDIKN
                     LVAIQILFGGGSPPFDIWIDDVALTR"
     gene            complement(9007..9261)
                     /locus_tag="VFQ61_37095"
     CDS             complement(9007..9261)
                     /locus_tag="VFQ61_37095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960185.1"
                     /translation="MRKLASQIAIDEPAEWANPTIIEIDSVTITGGATALGPYTFDAS
                     VPPMSVSRRIVGSTLTYREQGLTSCGPPSVQSSIRHAFLS"
     gene            complement(9863..10936)
                     /locus_tag="VFQ61_37100"
     CDS             complement(9863..10936)
                     /locus_tag="VFQ61_37100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960186.1"
                     /translation="MRVSSSRVTQATFVATLTLFACSGVDSDPFNPGAGSSGAPTVPN
                     GGGSGGSNAAGGASSTGSGGMISSAGSLAGGTSGALSTGMGGINPGSSLGGASSGRGG
                     ETSSGGVTSGAGQANGGSTLNGGSGPTSGGRSSGEGASSQGGRAASGASGIGGQTNAS
                     GAPSAGGGSGGGAGGSTGPGGVTCPFHGNITYTLAKSANPTAVEQDAYTRIAAALDKA
                     IQYYNCYTDITKKLNVSYVPSVQTADGNINGSMRFGSNTTYMDYRTAMHEIGHTVGVG
                     QASNWGSFVTADKIFTGQNANAELKAIDASLDAPSGDGKVHADNQHFWPYGLNQQNEV
                     KSEADLLFHCRMVMAIRKDLGLK"
     gene            complement(11113..12009)
                     /locus_tag="VFQ61_37105"
     CDS             complement(11113..12009)
                     /locus_tag="VFQ61_37105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960187.1"
                     /translation="MKLAAWKSSFITCIGLFASSAALNGCDATSKVRRTEYFGTVSLT
                     TGSEANYATQPLGIFYRVREQPEQGTCDVALTNGICRVWACDEASYEEPKPFPALDPG
                     KVTIAGSLEKDLKLTPDETGVLTASDLPTPLWETGATLTLNVEGSPDFPPLSVQLDAP
                     PKLTVLTPELPAPATDETNTEEPAPSSTEPLVIDPSKPFEIAWSEPKTGYIYVAISPE
                     TQASDEAKPIVTPGIDCQFSAADGEASVGTNVLEALPKPEYLTRYFLDVVTMSSDTTL
                     TDNARAEFRASWVGLTTPARVE"
     gene            12235..14082
                     /locus_tag="VFQ61_37110"
     CDS             12235..14082
                     /locus_tag="VFQ61_37110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9960188.1"
                     /translation="MSRTAPAQPKGHRFGLRAQLLVAVSVTCFCIVAGAAALQFMFSA
                     NQRRAEWVWHSQEVIAASQRLGRTASDAQTGMRGYLLTKRLAFLAPYYRGVDVLTSGF
                     PELARSVSDNAPQVQLVHKMQQAFEHWNVEVAQRWLNQASSLDAEQSEQTLQRGDRYM
                     NELRDTLEQFVAAEENLLEQRRTSSNVASRWSALAATAGFGLALLVVAVAGMGVVVRV
                     RRSVRELEQAAEAVARGELSRRAPQTGHDELASLSLAFNEMAGRLDQREKEENALKKL
                     RDMMQSSHSSEEAISLLARLGPACLPARSAQLFVMSFSKNLLDPAVSWGTEHESFNAF
                     APEDCWALRSGRVHCVHDPSDLACAHQTERAGMRACLPLVAQGETVGLLYVAPHGPEL
                     TAVAQEIAGVLALALANLRLREVLRNQAIRDPLTGLFNRRYLEETFERELARAKRTNV
                     PISIAVLDLDHFKRFNDTHGHAGGDALLRAFGAALRTMFRADDVLCRFGGEEFVVILP
                     SCTLHDAIARVDAFRASMASLEVQSEGRSLGEVTVSAGIANFPAHGDDRDKLFRLADR
                     ALYRAKRAGRNRIEVVESFSAELGHAPGESGSMGDQLWSEWQSRAKSGN"
     gene            complement(14121..18290)
                     /locus_tag="VFQ61_37115"
     CDS             complement(14121..18290)
                     /locus_tag="VFQ61_37115"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HET9960189.1"
                     /translation="MQLAAKFGQRRWRIFLLAGAVLFCAIVVGYYVATRPQPPTRAAA
                     IQARLELAAGEVGLETDQATAPAISGTPLRSGARLKTGKGARARVRLPDGASLFLRDN
                     TQLELQESGVSLGAGEYWLDAPPGEASQIRHQLGDVEVFAADSGLSLARSPAEVTVYV
                     ARGMATVTAPGGRVEVRAGERAIVKGKQAPAVAPVGFWDDWTGGMADFGSLASSGAAG
                     SGVIYGVDAAMRSGERPFHLEITKQSVRAKIREGLSETEVDQTFFNSQERDLEGWYWF
                     TVPERASVTGFAVETDGQLVWGEFIERKTAAQQYIAAKTSGHSPALLEWVDGHTYRAR
                     IYPIVAGRSRRVVLRYVELLTRRGETLEYVYPMAQRNPARIGEFSLTVDLGDDGQHMQ
                     LATRADARVERGGRSVSMRRSGFLPRADFQLEARLKKKKSAPLRLARYEVPGGESADY
                     LMVRYLPDLEWSGSQAPRGDVVVVVDTSAGSDEATRKLQVATAQAILRALSTEDRFAL
                     ISLDSKPKVLHPEMGLVAASDGEIEKALDRLAEQGSGGASDLSSLFEVALSRLHGAEQ
                     PAVVYVGDGLATTGELTHDKLIERLRRALSSSRARLFTVAVGNNADAALLGDLARTGG
                     GRGFLVEDGDQASARALEFAAALKVPTITDLELDLGAGLDEPYSNALGKISRGEEVVV
                     LARSHHDLPSKITVRGRLFGKTFSKDYEPEAEAALLGSHVPRLWAAEAMRRLLGSARG
                     PDAERGRIAALGVEYGLMTPFTSLLALESENAYVEQGIPRRNSALRGQILGALEPDIG
                     SSGASGRSAPAFMFGCSKSAPYSSEASPVAARKAEPAAASAPVVPEAAQDKSAEQESQ
                     SQAALRTSPGGGAAAPAEENQASLAPRDADDRAADPEFGIALGQRTASAPAPRSRAAA
                     GNPRKPVADIAQPKAKPAPPQPTPRAAVALRRILNLRLGTCSDAAERPLPERMLLWNK
                     RLLTAKDPEDLLSRYELAVRACEVPDWRAERALLELLSLRIRDEGTVTAVLSAFADRP
                     DVQRYLAKLILRRAVDPRLIQAVERAVFGGRVDWLKVDLETAKIASAEQRLTTLRQYV
                     AMAPDDPNGLLRLMRTLMDLSRTEEALGLGRRLRDQGFVTPLIAQQLGDMLARAGLAD
                     QAVRTYSEIVEFDAQNSASRSLLGDIYLAHGWYEPAYLEYRTLTEVEPENALPWLRLG
                     SAAAGAGRVDEALRVARRVMNAQGSPGPTDPRRWARLLSAAWLSRLIAESKTDAALEG
                     RRAAMQRELKELQLFSGPARVVLLSWQDYASELALVAGSGPSSGPTVTAQAGEARVGS
                     PIEARAVGLAGLELAATDRRDLGFIAELRSPPRDSDLRITLHDIFWDGKSFEIRVSSH
                     SLGARSTRLTL"
     gene            complement(18393..19586)
                     /locus_tag="VFQ61_37120"
     CDS             complement(18393..19586)
                     /locus_tag="VFQ61_37120"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET9960190.1"
                     /translation="MFQGKAGSPRRYYPALDALRSLAIAAVVWHHSTPRQLEGWPGQG
                     HLGVQLFFCISGFLITRILLLDCSEYGRVRLVHFWARRALRIFPLYYVVLAAFTLAAL
                     LMSTRGEGVTDAVGRGFLANFPFFVSYTSNWFVDYGVAHPVMFGFSWSLATEEQFYLT
                     WPVLVTLLGAHRAWLAGCALLLPFAISLLGSSAWAANMSPTGHRILASVSPCLYFGSS
                     AALVKDWFEEQRNLRIRAPFTRARSRGLWALLATLLTLLVAGFVYSPRVSFRGFEALL
                     AVWVLTLACAEDSSWLARLGTGKFGELGRLSYAVYLFHVPVIGFFRWAIPGIREEPLI
                     VFPAALLATWLAARASQRWFEVPISRLRERWFPHAGQPRWRSRSLGSLERDSVTPFPH
                     PPLSR"
     gene            complement(19621..20187)
                     /gene="thpR"
                     /locus_tag="VFQ61_37125"
     CDS             complement(19621..20187)
                     /gene="thpR"
                     /locus_tag="VFQ61_37125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET9960191.1"
                     /translation="MRCFFAVELADAVLEEIERALRPLRQQVRARWVARDKQHVTLVF
                     LGDLAPEQLPYIQEAGARTARGVLEFKLGVGAASGFPNPHHPRVLWLGLTGQLSELQT
                     LKASLDKALIPHGVTPETRAFRPHLTLARSAREGGDARLATCIAALEGSSFGSSPITH
                     LCLFESRQGADRYLLRGRYPLTPASERA"
     gene            20402..>21247
                     /gene="ttcA"
                     /locus_tag="VFQ61_37130"
     CDS             20402..>21247
                     /gene="ttcA"
                     /locus_tag="VFQ61_37130"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HET9960192.1"
                     /translation="MSNQLRRLHGYLMRTLRETCARYELLTPGDRVGVAVSGGKDSAT
                     LLVLLQELVRTLPFSVDLMALHLDQRQPGYDGRPLVRWLDERRVGYEILSEDTYSVVV
                     SKVGPGETYCSLCSRLRRGVLYTALERLRLNKLALGHHRDDGIETLLMNLFYAGRMQA
                     MPAKYTTDDGRHEVIRPLIECAEHSIREYAELCDFPILPCNLCGSQPGLRRDRMKQLV
                     DELEREHPNVRSVIANAIANVRPTHLLDADVARAWRERAPDLRPASEPAPSVKRMSAL
                     PLATSS"
BASE COUNT         3728 a         6706 c         6819 g         3994 t
ORIGIN      
        1 cacgcgccca aacgcgagca cttgaaacca ggagctcgcg aacatcatgg ataggctgag
       61 tgacttctgt gtgaacgcgg cgatatcgtc ggcgatgcgc tggtcgggat tgtcgatctc
      121 tgggctgccg agcaaatgat agtaggctcg attatcgaag tacttgccta agaagcggta
      181 gctgagccaa cgtcgccaac caatcgcgag cttgtcccgg acgtagtagt agaacgcgaa
      241 gatcggcacc gcgaagacga gcagcgcgag gaaggtgcgc atcgcgcgaa agaacctcgg
      301 cgcgtcacga gccgcgagcg cggacgtgaa ttcacccgac tgctcgttga aacgcacgtt
      361 gaactgcgtc tccagtacga gcagcaccag gaggagcgcg aacagccccc gggctcgcca
      421 cttttcctcg gagaaccaat acggtttcga aatgacgaag aagcgttcga cgagcttgag
      481 gttgagtccg tacatcgaag gtgtcgggcg cctaacacgt tcagcgtgag gcgcaggagc
      541 gagcgagaat tcgaggcgcc gcggcgcagt gtaccttgct ctcgtgctcg gcgcgtacta
      601 tccggcagcc gtggatctct cggccctcgc gttgcgcggt ttgctaggtt cccgcaagcc
      661 cattcatacg ggacgattca gggtcgcgtg tcagcgagag ctctcggtgc gccgagacgg
      721 tttcggcgtg gcctacatag acgcccagaa cgaagctgac gcctggttcg gccttggctt
      781 ctgtcacgca caggaccggg cggggcagct cgaggtcacc ttgcgcctca cgcgcggcac
      841 actctccgag ttagtgggtc gagaggggct tcacgtggac cgagcggtgc gcttgatcgg
      901 attctctcgc gcggccgccc ggcaactcga gaccttcgac ccgcagactc gggcgcagct
      961 cgaagcgtat actgcaggga tcaacgccgc gttcgcgtgc ggggccgcgc cgaggagcca
     1021 cgagcatgtg ctcctgcgct ctccgatgac gccttggcaa cctgccgatt gcatcgcgct
     1081 cggcctgctg acgtgctgct ttctcccgtc gaactgggac ctcgaactcg cacggctcct
     1141 gatctggtgc catgacggcc cggacgccgt cgcgaaagtc gatccgaact tccggcgcca
     1201 tccctcgaac ggccaaacgc cgagcgagct gttcctagcc gaagacttga gcgccctgca
     1261 gcgctttttg ggcccattgg gcggctcgaa cgcctgggcc gtggcgccgc acaagaccgc
     1321 acacggccgc gccttgcttg cgaacgaccc gcacttgccg gcgaacttgc cgaacctcgg
     1381 ctacctcgcg cgcgtcgttt gcccgagctt cgaggtaacg ggggttagcc tgatcgggat
     1441 cccagctttc attacgggtc acaacggata cgccgcttgg ggcaccacgg cggctcacgt
     1501 ggacaacgtg gatctgttcc tggaagagct cggcccaggc gggaaaacgg tgcgtgaagg
     1561 gcaagacttc gttccgatcc aagtcgagac ccagagcatc gtggtgaagg gcgaagctcc
     1621 gctcgagttg gaggtggcat ccaccgcccg tggcccaatc gtggcgcgca gctcgagtcc
     1681 cgagcaaggc ttctttcggc atgtacccgg ggtcggaggg gccaacgcgc tgtccttcgc
     1741 ggcgacctgg ctcgagccgc gtccgacgcg tgccctgctc gggctgcatc acgtgcggag
     1801 cttcgaagag tttcgggaga cctgtgcggc cgccaccggc tgtagctaca gcttggttta
     1861 tgcggatccg gagagcatcg gttggctgat ggcgagcgag gttccgcgac gcaagagcgg
     1921 gtatggctcg ctgccattgc cgggttggtc cccccacgtg ggctggatca aaccagcgat
     1981 tccggcgcag cgtttgccct ggtcacgtaa cccggagtcg ggtttcgtga gttgcgcgaa
     2041 cgacgatccg gttcgagagc atgcggagga cgtgttcctg ggtcacgatt tcctcgatca
     2101 tcagcgcaag acgcgcattg gcgaggtgtt gcgcgagggc tcggagttta caatcgagag
     2161 catgcagcgc ctgcagatgg acgtgctgag cattccgttt cgggaacttc ggtcgagctt
     2221 gatcgagctg cgccccaagc aggcgctcgc gcaacgggcg gtcgagttac tggcggcctg
     2281 ggacggccag gccacgagcg attcagtggc ggcgacggtg ttcgagttgt ttctgagtga
     2341 gctcgggcaa cgcgtgtgcg cgcaggccgc gccgcgggcc tggcagttcg cgctggggca
     2401 aagtgtgagt cacctaattc cgggaactac cttcaactat cggcggggga gcttcttggt
     2461 ccggtggatc cgcgattctc tgacgggaac gagccgtgaa tcattcgcgg tggaactagg
     2521 tgacgccctt cacgacgtga tgctgacgct gcgtacgcgc ttcggggagc gcgaagcaga
     2581 ctgggtctgg ggtgcggtcc gcccgttgca gctgcgacat cggctcggca ccgttcagcc
     2641 gcttgccgct tggttcaatc gcggccctta ccgggggctc ggcaacagca cgaccatcaa
     2701 catggcgagc gtggatatcc gtatgccgtt gcgaacaccc accatcactg cgcacctgcg
     2761 ggccgtttac gagattggga atttcgaagc gtcgcgtttc gtcttgctag gaggccagtc
     2821 cggcaatccg ctctccgccc actacaccga tttggtgccg ttctggcaac gcggggaggg
     2881 ggtgcccatt cactgcgcag aggccgccat cgaccgacac gccgttgccg agctgcggct
     2941 ggttccggcc tgaccgcgcc gagacagcta ttcccaagtg gttctaaact tgccggcggt
     3001 tgcgagcagg cccagcaagt acactccttg ctgataatat cgatacttac cggattgctg
     3061 atccacgagc caaagattcc tgacgaattg ggcgcggttc ggggcagcgg agccgaggct
     3121 cgcggcggcg agcgtggcgg tgagcgcggt ggaaccgccg cccgtgccgg cgctaccgtc
     3181 cactgcgaac agactattgc tgacgttgcc ctgcacgagc tgccccgtga agaacgcctg
     3241 gtacttttcg agctgcgcct tgagcccagg atctgcgctg aaccaggcgt agtcgagcgc
     3301 catgttcaag ggcacccgcc aggcgtcgta cttgaaacga tcgtggccgt ctcctgttgc
     3361 cgcggtgcgt ggggcaccgg cgaaggtcgc gtagtccgga tgaagtcccg tgctggggtg
     3421 ggccgacgcg gctaggaacg tgcggctgat ttgggcaatg tttttccaac gcgcacgatc
     3481 gctctgcggg ccccgctcgg caaagagctc gtagaaggcg ggcaggtgat agctcggatc
     3541 cgtaaacccg ttggacgcgc cgtagggcac gaacacgacc atgttctgag tcgggtgaat
     3601 gatgggatgg cgatcgtcgt tcgtgctcgc gttgtgaagc atggcactcg cgagagcgtt
     3661 cgcctcctgc aagtagttga ttgcacccgc gctcccccag cgcttgtggg cgaggtagag
     3721 cgcggccgcg aagtattctt ccccgtctgg ggccggagtc gttgtcgggt tgtagtctat
     3781 tttccagaga gcagggtcga gcgcgttgat tcccgtctct tgtatgcttt gccaacggaa
     3841 gtagtagcgc caagcgggag gatccgtatc ggcggtatac tgcatcttgg tttttgcgaa
     3901 acgccacagc ttgtcgaacg gggtctgcag gttcatctgc accgcgatca tcatgccgta
     3961 ggacattcct tcgcttcgga tgtcgttggt gtcgggggcc caaatgaagg ccagctcggg
     4021 attttggggt agctcgaagt agcagcggta gcccgtgttc acgatcaact cgttcttctc
     4081 gcccgtgccg attccgaaga accgattcac tgcagtgctg accttggctt cgacctgggc
     4141 cggatcttcg tcgagcaagg tgacgaacaa attggccgta gcgcgcggca gaatctcgat
     4201 actgccggta ctcccacctt cggacaagga acctgcatct ccgccactcg cgttcgtccc
     4261 gccgaagttg tctcctccca ggccgttttg ccctgctgtt cctcccgtgg ctcctcctgc
     4321 cgcggtggct ccagagctca tccctccggt tgaagtgcca cctgccgtcg ctccgccgct
     4381 tgcgcggccg ccggacgcct ccgtgtctcc gccttggccg gcgtcctctc cgctggttcc
     4441 accacgcgct ccgccactcg tcgcttccgt cgtcgtggtg cgagtcccac cctgcgggtt
     4501 gaaggtgccg cccgcgctcg cgcggccacc tgaactgcga gcatcgccgg actgacctgc
     4561 gttgccgttg ggcgcgccgc cactcccacc ctctaggctc gcgccagagc ctccgcctgg
     4621 gttcgcggtg ccacccgaag aatcgtcggt tctgggtggg agcggtgaaa cgcacgcgca
     4681 gagcacggca ccgataaaca atgagaaacg ggtcgacatc gattcgaagc tccgagcgat
     4741 tgcgggtgag cgaacaacgt cgatacgcgc gtcagggcgc tcgctgaagg gcggccgtgt
     4801 cggttatctc atgcgtgacg ggacatcatc cgtgctcgcg tcatccgcga ttgcgcgggg
     4861 gccgtcggcg cagtcggaac gctccaactg accctaatgc caacaaccaa cttgcgccgg
     4921 agtaggcctg cgtgcgagag gcggccctgc agtagcaacc cggtatcact gggtactcgg
     4981 gggctcgacc cgagacgctg cctccgagcg aaggcgtggg atcggtgctc atcgctccgc
     5041 ctgcagcccc acccatagcg ctgctgccgc cggaagccgc gcggccaccc gaactggtgg
     5101 tcgtctttcc gccgtttgga gttgcgctgc ttccgccggt ctcgggatcc accgcgctgg
     5161 ctcccgcggt cggttcagtg tcttgcccgg cgcttccacc cgcgtcggcg cccgcccctg
     5221 cgccggtacc gccggcaccg ccttcgccaa cggggaggtc aggaggatca tcggtcccca
     5281 cgaaggtaat gatgctgctg gccggcaaag tgtattcgaa cgagttggtc gccgtcacca
     5341 ctggctgcgc ttcgagctga ataccctcgc gcgtaagcca cggtcgcaga cttccagaag
     5401 gcagactctg gaacgcaaag cgctgcgtga cgggatagtc gttgtcgttg aacgttacga
     5461 tcgcgaagcg ctcaccgtcc ggatccgcaa acgccgaaac cccaattccc gtgctggggc
     5521 cacgaggctc tgcttggact ttatagaatc ctggccgcac gaacttgctg aagtgtccaa
     5581 gcccgtaggc ttgcggtagg acctcgccat tcttcagcaa actcgtttca ttgtccgagt
     5641 tcatccacca gtagttccag gcgttgacac gagcaatcga gagatggtcg tggatcatac
     5701 gtgcggtcgc cagcgcggcg ctgatgccgc ggctgtcgct gtagctgatc tcggtctccc
     5761 agaactcctt ctggtactcc ctgattttcg gatagtcgaa cggaaatccg ccatacgcgt
     5821 gagtggccac gatgccaaga aactcacggg cttgctcgtc ggcgagcagg gcatcggtgt
     5881 agctgcgcaa gcccgtccaa ttggcagact cgggcgccat caacagagtg ttggggctcg
     5941 cgtcttggag ggcagggccg aggtgatcgc gaatgaacgt gacgagctcg gcgggagtcc
     6001 agttgcaggt ttcccactcg gcggtccaat tcggctcgtt ctgagcagat agggcaatca
     6061 gctccacgcc ttgctgtttc atctcgcgcg tgaacgcagc gagccgctcc gcccacgcct
     6121 gatagtattc gggcaacagc gagccaccgt gattgtcggt cccgttcgtc ttccattccc
     6181 cgggcggcga ccaaggcgcg gcccacaccc ggacgccgcg ctcttgagcg cgcagcgcgg
     6241 ttcgagtctc gagggtagtg ccgtcgggcg cgatgcgcag gcgcaacaat gaaatgccga
     6301 gcccgcgctc ttccgagaag aagaagtccg cctgctcatc cgtcaacgtc ggggtcgtcc
     6361 aggcggagga cgtaccgaag cccgagatgc gttgttcacg ggtcccgaag tctaccgtca
     6421 cggtggttgt gctggcgtcc actgcacgct ggactgcagc cgctgacgtg tgctgcgggt
     6481 gcgactctgg gctcgaagtg cagctcgcga gtagtgcgag tgaggtaaag taagtggcgg
     6541 ttcgcacgtg aacgattccg agcctgccgt ttataggcag gctggtgatc cccctacggt
     6601 gattgaatgc gtgtggtgag cgggcagcta gaatcgcgcc tgccactgcg ctcgaattcc
     6661 atcgtgcgtc ggtaccaccg cgacactggg ctccggagat tcttcgcggt tacgcgtcaa
     6721 aacgtaccag agcgcggcgc cgccgaggcc ggctgcgcca acacccaagc ccacatacga
     6781 aaccaggaat ttggtgtgga tcttgtcggt ctccgcagga tcgcagcgtg ggctgcacgt
     6841 cttgccgagc tcattgtagt cactcgtacc cgtgacccgg aagtaagcga atgttccgag
     6901 ccccaagagc cccacaccac cgagcacgat actcggaatc gggacggagt tggggggagg
     6961 tgctgcaggc ggcaaggcac tgccggccgc ggccgctgag ccctccggtt tatcggcagt
     7021 cttcggtcgg acatccccga gcacgatttc gagcaggcga tttcgatcgc ccacccgcgc
     7081 ggtgtgcttg gtctcgagag ggggcaatcc ttctgattcg aaatagaagg tatgcaggcc
     7141 cgggttcatc tcgatcggtc tgccgtcgag gtgatcggtg acgagctgcc cgtcgaccgt
     7201 caccttgacg ttcacgagct cttcgccatt gccgttacgg accgagaaca ccatcgaggg
     7261 cgtgtcggct tgaatctgct catagagctt gatgcactcc tggacgagca gcgcgttgca
     7321 ttcggcttgc gagcaagtct gcgccgcgcg gctcgcctcg cggaagtcac ccttggcgcg
     7381 ctggcgctgg gcctcttcgt acgccgacgc gcattgttgg ctactttcct gcgcgcgcgc
     7441 caggctcggg cttccgagca gcgctgcggt gccggtagtg atcatggccg cgaaagccca
     7501 tttatttgga tacatcatat atcgactcat aaacgatgag aatctgactc agaacctgga
     7561 ttgcgctcgg caatatggcg acgaagcggc gctcaacgcg tcagcgcgac gtcgtcgatc
     7621 cagatatcga atggtggcga accaccgccg aacagaatct gaatggcaac caggttcttg
     7681 atgtctagct cggctggctc cggcatgttg ccgggctcga gcacgaagtc cgcgaacgac
     7741 agcgtgtagc gcttccagtc tccggagatg gtgacgatct tgtaccagtg tttgtcgcaa
     7801 gtgttagtcg cggtacagat gccgcccgct cgagaggtgt tcttgtctgc catctgcact
     7861 ttgaccgtgg ctggcttgtc gcctcgaccc cagaaagtaa tcccggtgtg gccgctgcca
     7921 tcccacagtc ctttggtgct ctcgctgccg ccggtgttgt tcagatctac gccaatgccg
     7981 ctgtagttct ggaaatcctg gcccgcgtag tggagcgcaa acttggaggt gccgcggtcc
     8041 ggaaacttcg gctcgaacaa ggacgtgccc gtctgatcgt cgaagcggta ccagttccct
     8101 gcgcggccgc tgatgcgagg aatgatgttg tctccgtcct cgaagttgtc gatcatgtcg
     8161 ccttccgcga tcacgatgtc gggcacgcag cgcgcggggg aaccggagca tacgaggctc
     8221 tcgcagtccg tgcccttgac gcacttcttg ccgttcgggc aagcttggtc cgggctgcac
     8281 acaccgccac agtcgatgtc actctcgtct tggttcttga cgtcgtcttg gcaagaaggc
     8341 ggcacacaga tgttgttctt gcatacaccg ctcgcgcaat cggtgatctt gaggcaagca
     8401 ctgtcgttgg ggcacgggtc gcacgtcgtg ccgccgcagt ccttatccgt ctcgtccgcg
     8461 tcgatcttct tgctcgagca gtggtcggtg cagacctcgt ctttgcaata gtcgctttgg
     8521 cagtcgccgt tcaccttgca ggcggtttca tcggggcaca agctggccgg ggcacaaacg
     8581 ccgccgcaat cggtgccagt ttcgttctga ttgaggatga ggtcctcaca gctcggttcg
     8641 gcgcagcgac ccttgacgca aagttccgtc gcgcagtccc cgtgtcgagt acagcgccca
     8701 ttgatctcgc atcgatcaca gagcgaggtg ccgccgcaat ccacgtccga ttcatcggca
     8761 ttgcgccgta gatccgaaca ggtatcgggc acctcggtac cggttgcccc gccctggccc
     8821 gtggtcgtgc cgcccgtgtc ccccgtggtg ccaccctgcg acgcaccgcc ggatgccgtg
     8881 gtgccaccac tgccgccggt ttgggcccgc ccgcccgaac cgaattgggc ggagagcggt
     8941 tccagatcgg gttcacaagc cgcaacgata ccgagggaag tcaacgcagc gagggagaag
     9001 cggagttcac gaaaggaacg catgacgaat actcgactga acggagggag gaccacaaga
     9061 ggtaaggcct tgctcgcggt aggtcagcgt gctgccgacg attcggcgcg acaccgacat
     9121 cggcggaaca ctcgcatcga aggtgtatgg gccgagcgcc gttgcaccgc cggtgattgt
     9181 aacagaatcg atctcgatga tcgtgggatt cgcccactct gcgggctcgt cgatcgcgat
     9241 ctgagaggcg agcttacgca acgctgtggg ttcgaagttg ccaccgtaca gcggaatcgt
     9301 tacatcatgc caccggccat ccgcgggctc gagagcgaag tccgaggata acctggggca
     9361 ttgaagtcgg aatctcgagc gtgcatgagg aagcctccgg atttgccggc cgcatgcgaa
     9421 tgcgagctgt gaccgtggcg cccggtgtgc gagagacgtc tcgcgcgtca tcgtaccaaa
     9481 tgtgcgccga tcagcgggtg ttcgggggct gaggggaacg ctgaagactg cacattcgcc
     9541 gcattcactt ggacaccccg gcgtttcccg ttgtgcctgc agcttccgat gctccgccgg
     9601 ttcgtcgctg acgagctgcc acacgaaggg tgcagcaatt cgaagaggcg ccgcggttgc
     9661 ataagtaccc ccgcttggcg gtcgcgccgc cgctcgccga tgagccacca ccggccgagc
     9721 ctccccttgc gctcacggtg gcgccggccg cggcttggcc gcgtgccgca ttgcccatac
     9781 cgatcctgcc ctcccgctgg tgaagtcggc gcctgccgcg tcggcgattc gagtcggccc
     9841 ccggcgctgc tcctcgcgcg gcttacttca gcccgagatc cttgcggatg gccatcacca
     9901 ttcggcaatg aaacaacagg tcggcctcgg acttcacttc gttctgctgg ttcaaaccat
     9961 agggccagaa gtgttgattg tccgcatgta ccttaccgtc accgctcggg gcgtcgaggc
    10021 tcgcgtcgat tgccttcagt tcggcgttgg cgttctggcc ggtgaagatc ttgtcggccg
    10081 tcacgaatga gccccaattc gaggcctgtc cgaccccgac cgtgtgccca atctcatgca
    10141 tggccgtgcg atagtccata tatgtggtgt tggacccgaa gcgcatcgat ccattgatgt
    10201 tgccgtcggc ggtctgcacg gacggcacat atgaaacatt gagtttcttg gtgatgtccg
    10261 tgtagcaatt gtaatattga attgccttgt ccagcgcggc cgcgatgcgc gtataggcgt
    10321 cctgttccac cgcggttggg ttcgctgact tggcgagtgt gtaggtgatg ttgccgtgga
    10381 acgggcaagt gacaccacct ggcccggtgc ttccacccgc gcctccgccg ctgcctccac
    10441 ccgcgctggg cgcgccagag gcgttcgtct gtccgccaat cccgctggca cccgatgcgg
    10501 cgcggccgcc ttgactggag gccccttcgc cgctcgaccg ccctccgctg gtgggcccgg
    10561 agccgccgtt gagcgtgctg ccgccgttgg cttgacctgc cccagaagtg accccgccac
    10621 tcgacgtttc cccgcctcgt ccgctggacg cgcctccgag ggaactccct gggttgattc
    10681 cgcccatccc tgtcgagagc gcgccgctcg ttccgcctgc aagcgagccg gccgaggaga
    10741 tcatgccgcc gctcccggtc gagctagctc cgccggcggc attgctccca ccacttcctc
    10801 cgccattggg tacagtgggc gcgccgctcg aaccagcccc agggttgaag ggatcggaat
    10861 cgactccgga acaagcaaat agggtgagcg tcgcgacgaa cgtggcctga gtcacgcggc
    10921 tggagctaac gcgcatggcc gttagctatc atggaatcgc agacccagct cgcgctcgca
    10981 gcgcagggat tgtgccgcaa atcagttgct tgaagcagca gagagcaagc gtgtgcgcag
    11041 ccaacgcaat ttgaaatcat tcctgtagct cagcgccggg ccacacctgg ccgcgacccc
    11101 cagcccgtcg gttcactcca cacgtgccgg agtcgtcagc ccaacccaag acgcgcgaaa
    11161 ctccgcgcga gcattgtccg tcagggtcgt gtcgctgctc atggtgacta cgtccaggaa
    11221 gtagcgcgtc agatattccg gtttgggcag cgcctccagg acattcgtcc cgacgctggc
    11281 ctcgccatcc gcggccgaga actggcagtc aatcccagga gtgacgatag gcttagcctc
    11341 gtcgctagct tgcgtctccg gagagatagc gacgtagatg tacccagtct tgggttccga
    11401 ccacgcgatt tcgaacggct tggagggatc aatcacgagc ggctcggtag aggacggcgc
    11461 cggctcctcg gtgttcgtct catccgtggc gggcgcgggc agctcgggcg tgagcaccgt
    11521 cagcttcgga ggcgcgtcga gctgcacgct gaggggtggg aagtctggtg agccctccac
    11581 gttcaacgtg agggttgcgc ccgtttccca aagcggagtc ggcagatcac tcgcggtcaa
    11641 gacacccgtc tcgtcagggg tcagcttcaa gtctttttcg aggctgccgg cgatggtgac
    11701 cttgccggga tcgagcgcgg ggaagggctt tggctcttcg tagctggcct cgtcgcaggc
    11761 ccagacccgg caaattccgt tggtcagcgc tacgtcacac gtgccttgct ccggctgctc
    11821 cctaactcgg tagaagatcc ccaatggctg ggtggcatag ttcgcctcgg aaccagtggt
    11881 taagctcact gttccgaagt actcggttcg acggaccttc gaggtggcgt cgcagccgtt
    11941 cagcgcagcg ctcgaggcaa aaagcccgat acacgtgatg aaggaggatt tccaagcggc
    12001 aagcttcatg cgcgtggtct tagccgcgaa gcggggctgg ttccagcacc gaaatgcaga
    12061 gttgccacgg ctggcaaagt agcctcgatt tagcgggccg cgcccctcgg ttgggaggag
    12121 gcatctagct gctgtagccc ttatgcactg tagggggggt gccgttgctg acgccgcggg
    12181 agcgaatggg gaccgcgagt ccgccgcagc tcgcctaccg ctgattttct gccgatgtca
    12241 cgaaccgcac cagctcaacc gaaaggccat cgctttgggt tgcgggctca gctcctcgtc
    12301 gccgtatccg taacttgctt ttgtatcgtc gcgggggcgg cggctctgca gttcatgttt
    12361 tccgcgaacc agcggcgtgc ggagtgggtc tggcactcac aagaggtgat cgccgcctcc
    12421 cagcgcttgg ggcgaaccgc gtcggacgcc cagaccggca tgcgaggtta cttgttgacc
    12481 aagcgactcg cattccttgc tccgtactac cgaggcgtcg acgtccttac gtctggtttc
    12541 ccagagctgg cccgctcggt gtcagacaac gctccccaag ttcagctcgt gcacaagatg
    12601 cagcaagcgt tcgagcactg gaacgtggag gttgcgcagc gctggctcaa tcaggcgtcg
    12661 tcactggacg ccgagcaaag cgagcaaacg ctgcagcgtg gcgatcgcta catgaatgag
    12721 ctgcgggaca cgctcgagca gttcgttgcg gcggaagaga atctgctcga gcaacgccgc
    12781 acatcgagca acgtggcgag tcgttggagc gcactggcgg ccacggctgg gttcgggctg
    12841 gcgctgctcg tggttgcagt ggctggcatg ggagtggtgg tgcgtgtgcg tcgcagtgtt
    12901 cgggagctgg agcaggccgc cgaggcggtc gcacgcggtg agctatcccg tcgggctccc
    12961 caaaccggtc acgacgaact cgcatccttg tcgctcgcct tcaatgagat ggcgggccgc
    13021 ctcgaccagc gcgaaaagga ggagaatgcg ctcaagaagt tgcgagatat gatgcagagt
    13081 tcgcactcgt ccgaagaggc gatttcactg cttgcgcggc tcggccccgc gtgcttgccg
    13141 gcgcgcagcg cgcagctgtt cgtgatgagt ttttcgaaga acctgctcga cccggcggtg
    13201 tcctggggca ccgagcacga gagcttcaac gcattcgctc ccgaggattg ctgggcgcta
    13261 cgaagtggtc gggtgcattg tgtccacgac ccttctgatc tggcttgtgc ccaccaaacc
    13321 gagcgcgctg gaatgcgagc ttgccttccg ctggtcgcgc aaggcgagac ggtgggcctc
    13381 ctctatgtcg cgccgcatgg cccagagttg acggccgtag ctcaggagat cgcgggcgtt
    13441 ttagcgctgg ccctcgcgaa cttgcgattg cgggaggtgc tccggaatca ggccattcgc
    13501 gatccgctca cggggctgtt caaccgccgc tatctggagg agaccttcga gcgtgagttg
    13561 gctcgcgcga agcgtacgaa cgttccgatc tctatcgctg tcctcgatct ggatcacttc
    13621 aagcgcttca atgacactca cggtcatgcg ggtggggatg ctttgctccg agcgttcggg
    13681 gccgccttgc gcaccatgtt tcgtgcggac gacgttctgt gcagatttgg aggtgaggag
    13741 ttcgtcgtca tcctgccgag ctgcacgctg cacgacgcca ttgctcgagt cgatgcgttc
    13801 cgtgccagca tggcctcgct ggaagtccag agtgagggcc ggagcttggg cgaggtgacg
    13861 gtgtcggcgg gaatcgcgaa ctttccggcg cacggcgacg acagagacaa attgtttcgt
    13921 ctggcagatc gagcgctcta tcgggcaaaa cgcgcaggcc gaaatcgcat cgaggtcgtc
    13981 gagagcttca gcgctgagct gggccacgct cctggggaga gcggatcgat gggggatcaa
    14041 ctgtggtcgg aatggcaatc tcgcgccaaa tccggcaact gaagctcccc cgcttcggag
    14101 gtcgaggccg cgcgtgagtt tcagagggtg agccgcgtcg aacgcgcgcc aagtgagtgg
    14161 gaagagacgc gaatctcgaa gctcttcccg tcccagaaga tatcgtgcaa cgtgatgcgg
    14221 agatcgctgt cacggggcgg gctccgcagc tctgcgatga agccgagatc gcgccggtcg
    14281 gtcgcggcca gttcgagccc ggcgagcccg accgcgcggg cttcgatagg tgagccgacc
    14341 cgcgcctcgc ccgcctgagc ggtgacggtt gggccactag acggccccga acccgcgacg
    14401 agcgcgagct cactggcgta gtcctgccaa gacagaagca cgactcgtgc gggaccgctg
    14461 aagagctgga gctccttgag ctcacgctgc atggcggctc ggcgtccctc gagcgcggca
    14521 tcagtcttcg actcggcgat caagcgggag agccacgctg cgctcagaag tcgtgcccag
    14581 cgcctcggat cggtcgggcc gggactgccc tgggcgttca tcactcggcg cgcgactcgg
    14641 agcgcctcgt cgacgcgccc ggcgccggcg gcggcgcttc ccagtcgcag ccagggcagc
    14701 gcgttctccg gctccacctc ggtcagggtg cggtactcca ggtacgcggg ttcataccaa
    14761 ccatgggcca agtagatgtc gccgagcagg ctgcgcgagg ccgagttctg cgcatcgaac
    14821 tccacgatct ccgaataggt gcgcacggct tgatcggcca aacccgcgcg ggcgagcatg
    14881 tcaccgagct gctgggcgat cagcggagtg acgaagcctt gatcacgcag gcgtcgaccg
    14941 agcccaaggg cctcctccgt tcgggatagg tccatcagtg tgcgcatcag ccggagtagc
    15001 ccgttggggt cgtcgggtgc catggccacg tattgacgaa gtgtcgtcag gcgctgttcg
    15061 gccgacgcaa tcttcgcggt ctcgagatcg accttcagcc agtcgactcg tccaccgaac
    15121 accgccctct ctacggcttg aatcagccgc ggatctacgg cacgccgcaa aatcagcttc
    15181 gcaagatagc gctgcacgtc tgggcgatcg gcgaacgcgg acaacaccgc cgtcaccgtg
    15241 ccttcgtccc gaatgcgtaa actcagcagt tcgagcagcg cgcgctcggc acgccagtca
    15301 ggtacctcac aagctctcac ggcgagctcg tacctactca gcaagtcctc ggggtccttg
    15361 gcggtcagta ggcgcttatt ccaaagcagc atgcgctcgg gtagaggtcg ctcggctgcg
    15421 tcgctacacg taccgagcct cagattgagg atgcgacgca gagcgactgc ggctcggggc
    15481 gtgggttggg gaggtgctgg tttggccttt ggttgagcga tgtctgcgac cggtttcctc
    15541 gggttgcccg ccgccgcgcg gctgcgcggc gctggtgcgc tggcggttct ttgacccagc
    15601 gcaatgccga actcggggtc ggcggccctg tcatccgcgt cccgcggcgc gagtgacgcc
    15661 tggttctcct cggctggagc cgctgctcca ccccccgggc tggtgcgtag tgcggcttgt
    15721 gattgcgatt cctgttccgc tgatttgtcc tgagcggctt cggggacaac gggcgccgac
    15781 gctgctgccg gctccgcctt gcgtgcggcg actgggctgg cttcggaaga ataaggagct
    15841 gatttcgagc agccgaacat gaacgcgggt gctgatcgac cgctggctcc actggagccg
    15901 atatcgggct cgagcgctcc caagatctgc ccgcggaggg ccgagtttcg acgcgggatg
    15961 ccttgttcta cgtaagcgtt ctcgctctcg agcgcgagca ggctcgtgaa cggcgtcatc
    16021 aggccgtact ccacaccgag cgctgcaatg cgtccacgtt cggcgtccgg accccgggcg
    16081 ctcccgagca aacgccgcat ggcttccgcg gcccagagcc ggggcacgtg cgagccgagc
    16141 aaggcagctt cagcttctgg ctcgtaatcc ttgctgaagg ttttgccgaa tagccgaccc
    16201 cgcaccgtga tcttgctggg caaatcgtga tgagagcggg ccagcaccac cacctcctca
    16261 ccgcgcgaga tcttacccag agcgttgctg taaggctcat cgaggccggc gccgagatcc
    16321 agttcgagat cggtaatcgt ggggaccttc aaagccgccg cgaactcgag cgctcgggcc
    16381 gatgcttgat cgccatcctc caccaggaag ccgcgcccac cgccggtgcg ggctaagtca
    16441 ccgagcaacg ccgcatcggc gttgttgcct acggccaccg tgaagagccg cgcgcgagac
    16501 gagctcaatg cgcggcgaag ccgttcgatg agcttgtcgt gggtcaactc tccggtcgtg
    16561 gccagcccgt ctccgacata gactactgcg ggctgctcgg cgccgtgcag tcgggacaag
    16621 gccacttcga acaagctcga gagatcactc gcgcccccgg agccctgctc ggcgagccga
    16681 tctagcgctt tttcgatctc gccatcactg gccgccacta gacccatctc ggggtgcaag
    16741 accttgggtt tgctatcgag cgagatcaac gcaaagcgat cctccgtcga gagcgcgcgt
    16801 aagatggcct gagccgtcgc gacttgcaat ttacgggtgg cctcatcgct tcccgcagaa
    16861 gtatccacga ccaccacgac gtcaccgcgc ggagcctgac tgcccgacca ttcgaggtcg
    16921 ggaagatatc gcaccatcag gtagtccgcg ctctcgccac cgggaacctc gtagcgggcg
    16981 agccgcagcg gagccgactt cttcttcttt agccgcgctt cgagctggaa gtctgcacgc
    17041 ggtaagaacc cggaacgacg catgctcacg ctgcgcccac cccgctcgac tcgagcgtcg
    17101 gctcgggtcg cgagctgcat gtgctgcccg tcgtcaccta gatccacggt tagcgaaaat
    17161 tccccaatcc gcgcggggtt tcgctgcgcc atgggataca cgtactcgag cgtctcgcct
    17221 cgcctcgtca agagttcgac gtaacgcaac accacccgcc ggctacgtcc ggcgacgatc
    17281 ggataaatcc gcgcgcggta agtgtgcccg tccacccact cgagcagggc aggagaatgc
    17341 cccgaggttt tggcggcgat gtattgctgg gccgctgttt tgcgttcgat gaactcaccc
    17401 cacaccagct gcccatcggt ctcgaccgcg aaccccgtga cgctggcacg ctctggaacc
    17461 gtgaaccagt accagccttc caagtcacgc tcttgcgagt tgaagaaggt ttggtctacc
    17521 tcggtttcgg agagcccctc gcggatcttg gcccgcacac tctgcttggt gatttccaga
    17581 tggaaaggtc gttctcccga gcgcattgcc gcgtctacgc cgtagatgac gccgctgcct
    17641 gccgcgccgg aactcgcgag gctgccgaaa tcagccatgc ctccggtcca gtcgtcccag
    17701 aacccaacgg gtgccaccgc gggtgcttgc ttgcccttca cgatcgcccg ttcacccgcc
    17761 cgcacctcca cgcggccacc tggtgccgtg acggtggcca tgccccgagc cacgtatacg
    17821 gtaacttcgg ccggcgagcg cgcgagcgat agaccgctgt cggcagcgaa gacctcgacg
    17881 tccccgagct ggtgccgaat ctggctggcc tctccgggcg gagcgtccaa ccaatattca
    17941 cccgctccga gacttactcc gctctcttgc agttcgagct gcgtattgtc cctcaggaaa
    18001 aggctagccc cgtcgggtaa gcgtacgcgg gcgcgcgccc ctttgcctgt cttgagccgt
    18061 gcgccggaac gcaggggagt tcctgaaatg gctggggccg tcgcttggtc cgtttcgaga
    18121 ccgacttcgc cggcggccaa ttccaagcgc gcctggattg cggcggcacg tgtcgggggc
    18181 tgcggacgcg tcgccacgta atatccgacc acgattgcgc agaacaacac ggcgcccgcc
    18241 aataagaaga ttctccagcg ccgttgccca aacttcgccg caagctgcat ctcgcactcc
    18301 tctggagggg ctttgcccct cgcgctcctg acgtgaccag acacgcaact gctgcgagtc
    18361 ttggatacat cgggcctccg accgggccca gcctagcgtg aaagcggcgg gtgcgggaag
    18421 ggcgttaccg aatcgcgttc tagcgacccg agcgagcgcg accgccagcg tggttgcccc
    18481 gcatgcggga accaacgctc gcgcagcctg gagatcggca cctcgaacca acgctggcta
    18541 gctctcgctg cgagccaagt ggcgagcaac gccgccggga agacgatgag cggctcctca
    18601 cgtatgccgg ggatggccca gcgaaagaaa ccgatcaccg gaacgtggaa gaggtaaacc
    18661 gcataactaa gccggcctag ttccccgaac ttgccggtcc cgagccgcgc cagccaactc
    18721 gagtcctcgg cgcaagcgag cgtgagcacc cagaccgcca gcagtgcttc gaagccgcga
    18781 aaagaaacgc gaggcgagta aacgaagccg gccaccagca gcgtgagcaa agtggccagc
    18841 agtgcccaca acccccgaga gcgggcccgg gtgaacggag cgcgaatacg caagttgcgc
    18901 tgctcctcga accagtcttt tacgagggcc gcgctcgacc cgaagtacaa gcacggggac
    18961 acgctggcga ggatgcgatg ccccgtcgga ctcatgtttg cggcccaagc cgagctcccg
    19021 agtagactga tcgcaaaggg cagcagcagg gcgcagcccg cgagccacgc gcgatgagca
    19081 cccaacagag tcactaggac gggccaagtt aagtagaatt gttcctcggt cgcgagcgac
    19141 caagagaagc cgaacatgac cgggtgggcg accccgtaat cgacgaacca gttgctcgta
    19201 tagctgacga agaacggaaa gttggctaag aaaccgcgtc cgacagcgtc cgtcacccct
    19261 tcgcctctcg tcgacatgag cagcgcggcg agggtgaagg cggcgagtac gacgtaatac
    19321 agaggaaaga tgcggagcgc gcgccgtgcc cagaagtgta cgagccgcac ccgcccgtac
    19381 tcactgcaat cgagcagcag gatgcgggtg attaggaatc cgctgataca aaagaacaat
    19441 tgtacgccga ggtggccttg ccccggccaa ccctccaact gccgaggcgt cgagtggtgc
    19501 caaacgaccg ccgcaatcgc gagtgagcgg agggcgtcga gcgccggata gtatcgccgc
    19561 ggggaacctg ccttgccctg aaacacgcgg catgattcca agccgagcca cggccgcgct
    19621 tcaagctctt tcgctcgcgg gggtgagtgg gtaacgccct ctcaacaggt atcgatcagc
    19681 cccttggcga ctctcgaata ggcagaggtg cgtgatggga gacgaaccaa agctcgaacc
    19741 ctcgagcgca gctatgcacg tggccagacg ggcatcccct ccttctcgtg cggagcgcgc
    19801 gagcgtgaga tgcgggcgga atgctcgcgt ttcgggggtg accccgtggg gtatcagcgc
    19861 cttgtcgaga gacgccttga gcgtctggag ctcactcagc tgcccggtca gccccaacca
    19921 gagcacccgc gggtgatgtg ggttcggaaa gcctgatgcg gctcccactc ccagtttgaa
    19981 ttcgaggacg ccccgagccg ttcgggcgcc agcttcctgt atgtatggca gctgctccgg
    20041 cgcgagatcg ccgagaaaaa ccagggtgac atgctgcttg tcccgggcga cccagcgcgc
    20101 ccggacctgc tggcgcaacg gtcgtagcgc tcgctcgatc tcctcgagga ccgcgtcagc
    20161 caactcgacc gcgaagaagc agcgcatcgt gcgcagtcta ggagcccatc ccaacagcat
    20221 ctagtcgctg gcagcccgta gctcgcgtcg gtttcaagaa gccgagccgc ttcgatgtcg
    20281 gccaccgtct ccggcgcccg ggatgggctc gcgagcctgt ccaggggctt cacgccggtt
    20341 tcgagctggg tccgaacacg gctggccggc gcgttcgtct ggcctgagct aaagagcagt
    20401 cgtgtccaac cagctgcgcc gtctccacgg ttatctgatg cgaactctgc gcgagacgtg
    20461 cgcccgctac gagctcctga cgccggggga ccgggtcgga gtggcggtca gcggcggaaa
    20521 agacagtgcc actctgttgg tgcttctgca ggagctcgta cgcacgctgc cgttcagcgt
    20581 cgacctaatg gcgctccacc tggaccaaag acagcctggt tatgacggtc gccccctggt
    20641 gcgctggttg gatgagcgtc gcgtgggcta cgagatcctg tccgaggaca cctattcggt
    20701 ggtggtctcc aaggttggac caggagagac atattgctcg ttgtgttcac gcttgcggcg
    20761 cggtgtgctc tacaccgctt tggagcgcct gcgtctcaac aaactcgcct tgggacatca
    20821 ccgagacgat ggcatcgaga cgctactcat gaacctcttc tatgccggtc gcatgcaggc
    20881 gatgcccgcg aaatacacga ccgacgacgg tcgtcacgag gtaattcgac cgctaatcga
    20941 gtgcgctgag cattccatcc gtgagtatgc ggagctgtgt gatttcccaa tcttgccctg
    21001 caacctctgt ggctcgcagc ccgggctgcg ccgggatcgc atgaagcaac tggtggacga
    21061 gctcgagcgg gagcacccca acgtgcgaag tgtgattgcg aacgccatcg cgaacgtacg
    21121 gccaacgcac ttgttggatg cggatgtggc gcgggcttgg cgcgagcgag caccggacct
    21181 acgccccgcg agcgagccgg cgcccagcgt caagcgcatg agcgctctac ccctcgcaac
    21241 tagctca
//
LOCUS       DASTKU010001049        13555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1548208, whole genome shotgun sequence.
ACCESSION   DASTKU010001049 DASTKU010000000
VERSION     DASTKU010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 13555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13555
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548208"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..864)
                     /gene="rpsD"
                     /locus_tag="VFQ61_37135"
     CDS             complement(235..864)
                     /gene="rpsD"
                     /locus_tag="VFQ61_37135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET9960193.1"
                     /translation="MARYIGPVCKLCRREGGKLYLKGERCFTDKCSVGRRPYPPGQHG
                     QARIKLSEYGLRLREKQKMRRIYGLLERQFASYYVAATHLPGRSGEEMLGLIERRLDN
                     VVHRMGLAGSRAQGRQLVRHNHILVNGKRVNIPSFQVKPEDKIEVREKSRKIAFIQAA
                     LAAAENRQRPSWIDVDKGSFSGVVKSMPVRDELNEPMVREQLVVEYYSR"
     gene            complement(879..1271)
                     /gene="rpsK"
                     /locus_tag="VFQ61_37140"
     CDS             complement(879..1271)
                     /gene="rpsK"
                     /locus_tag="VFQ61_37140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET9960194.1"
                     /translation="MATPKARPAKKTVKKNIFAGIAHIQSTFNNTIVTLTDVNGATIS
                     WASAGSRGFKGSRKSTPFSAQLAAEEAGRRAMEHGVRSVAVFVRGPGAGRESALRALQ
                     QVGFKVTLIRDVTPIPHNGCRPPKRRRV"
     gene            complement(1295..1660)
                     /gene="rpsM"
                     /locus_tag="VFQ61_37145"
     CDS             complement(1295..1660)
                     /gene="rpsM"
                     /locus_tag="VFQ61_37145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET9960195.1"
                     /translation="MARIAGVDLPRRKQILYALPYIYGIGRATAARLCSIANIPATRR
                     VEELSEADVKAIRDAIEANVKVEGDARRDVQANIKRLMDLGCYRGLRHRKGLPVRGQR
                     TRTNARTRKGPRKNLGRRA"
     gene            complement(1691..1804)
                     /gene="rpmJ"
                     /locus_tag="VFQ61_37150"
     CDS             complement(1691..1804)
                     /gene="rpmJ"
                     /locus_tag="VFQ61_37150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET9960196.1"
                     /translation="MKVRPSVKKICDKCKIVKRRGVLRVICDNPRHKQRQG"
     gene            complement(2048..2671)
                     /locus_tag="VFQ61_37155"
     CDS             complement(2048..2671)
                     /locus_tag="VFQ61_37155"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET9960197.1"
                     /translation="MRLILLGAPASGKGTQAIVLCERFSVPKISTGDMLREHLAQGTA
                     LGLEAKNYMSEGKLVPDDLVIRMVEQRFLAPDAAKGFVLDGYPRSVPQAEALSGLLTK
                     LGTKLDAVILIDVAHELLVERATLRRTDKRTGQIYHLKYNPPPPDAELEHRADDQEAT
                     VIKRLAQYDAVTAALVPFYSAAGLLRRVDGVGSPKEISQRILSTLES"
     gene            complement(2668..3999)
                     /gene="secY"
                     /locus_tag="VFQ61_37160"
     CDS             complement(2668..3999)
                     /gene="secY"
                     /locus_tag="VFQ61_37160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET9960198.1"
                     /translation="MAVLQGFSNIGKVPELRQRILFTLGVVAIYRVGVFITTPGVDRS
                     VMRNIVKTQGGLLGFVNLFSGGALQNLSIFALGIMPYVSASIVMQLLGLVSKQVEEMR
                     REGEAGRRKLEQWTRYGTIVLSLFQGFGVASMLEGMNNSDMGGGRFGDVVADPGWGFR
                     LMTVITLTTGTGLLMWLGEQATERGIGNGTSMIIFASIVSGIPGGIMNYIAGNTGDIQ
                     PLTITILVAVLVMSVACVVFFERAQRRIPIQYSRRQVGRHVYGAQRAHLPLKVNMASM
                     IPAIFASSLLMFPATLANFNIPGMQRLSGVLNRGDWAFQTVFASLCIFFSFFYTAVTF
                     QPVDVAENLKKQQANIPGVRPGKQTAEYIDRVLTRLTVAGSIYVAAICVIPSVIATSF
                     RVPFQFGGTSLMIVVGVALETVNQIEAHLITRSYEGLTGPRMTRLSGRRTT"
     gene            complement(4030..4527)
                     /gene="rplO"
                     /locus_tag="VFQ61_37165"
     CDS             complement(4030..4527)
                     /gene="rplO"
                     /locus_tag="VFQ61_37165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET9960199.1"
                     /translation="MSTDLSRLAYPEGARKSEKRLGRGIGSGLGKTCGKGSKGQKARH
                     GGDFGKLHFQGGQTPLQRRLPKRGFRVPFPIKTVGVNIGVLEDFAEGSVVDEAALRDA
                     RLVQGRGVRIKILGDGDLSKKLTISAHAFSKSALEKIEKAGGTATVLAFGPATEATEA
                     SEGQG"
     gene            complement(4524..4712)
                     /gene="rpmD"
                     /locus_tag="VFQ61_37170"
     CDS             complement(4524..4712)
                     /gene="rpmD"
                     /locus_tag="VFQ61_37170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009336598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET9960200.1"
                     /translation="MATKLEVRQVHSEIGRPHPQRRVLRGLGLRGPGSSVLVDNTPSF
                     RGMIKKVLHLVKVEEKQS"
     gene            complement(4802..5299)
                     /gene="rpsE"
                     /locus_tag="VFQ61_37175"
                     /pseudo
     CDS             complement(4802..5299)
                     /gene="rpsE"
                     /locus_tag="VFQ61_37175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            complement(5319..5678)
                     /gene="rplR"
                     /locus_tag="VFQ61_37180"
     CDS             complement(5319..5678)
                     /gene="rplR"
                     /locus_tag="VFQ61_37180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026114014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET9960201.1"
                     /translation="MAAKLTGRERRKLRIRSRITGTEERPRLSVFRSGRHIYAQVVID
                     TDGRTVASASTLSRDLKGTLTEDAKSDAARKVGALIAKMCLAKNVEKVVFDRNGYLYH
                     GRVKALAEAAREGGLNF"
     gene            complement(5700..6293)
                     /gene="rplF"
                     /locus_tag="VFQ61_37185"
     CDS             complement(5700..6293)
                     /gene="rplF"
                     /locus_tag="VFQ61_37185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET9960202.1"
                     /translation="MSEQTQQAAPKRLSRVGKRPVVLPKGVAVNVAGTRLEIQGPKGK
                     LAMDLPPNVSVKKDGDKVLLESDAEGRDAARLQGLARALLASRVKGAAEGYERQLELV
                     GTGYRAEVKGQVVTLSLGFSHPTQIQLPAGVTGNVPPDSKGTMLVLNSADKAALGQIA
                     ATIRDLRPPEPYGGKGIRYRGETIRRKAGKAAKGKGK"
     gene            complement(6293..6697)
                     /gene="rpsH"
                     /locus_tag="VFQ61_37190"
     CDS             complement(6293..6697)
                     /gene="rpsH"
                     /locus_tag="VFQ61_37190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET9960203.1"
                     /translation="MATVNDPVSDMLARIRNAALARHELTRLPASGLKKGIAEILKAE
                     GYVADVRQEEWGPKKHSTLTIVLKYNNRESAFQGLRRVSRPGRRVYVRHTDIPRVLSG
                     LGVSILSTSHGLMTDREARQRKIGGELLCEVW"
     gene            complement(6697..6882)
                     /locus_tag="VFQ61_37195"
     CDS             complement(6697..6882)
                     /locus_tag="VFQ61_37195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET9960204.1"
                     /translation="MARASQWAKLNRPPKFSTRAVSRCRVCGRSRAFYRDFNLCRLCL
                     RSMALRGELPGVTKASW"
     gene            complement(6887..7441)
                     /gene="rplE"
                     /locus_tag="VFQ61_37200"
     CDS             complement(6887..7441)
                     /gene="rplE"
                     /locus_tag="VFQ61_37200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET9960205.1"
                     /translation="MTDVVEPRMRERYKKDVVPGLMKQFGYGNPMQVPRLKAIVLNMG
                     LGEAVANPKLIDAAVAEVTSITGQKPVVTRAKKSIATFKLRENMPIGVKVTLRRERMW
                     EFLDRLVTLALPRVRDFRGISPRGFDGAGNYTLGLREQIVFPEIDFDKVEKIKGMNIT
                     FVTSAETDEEAKALLGSLGMPFRS"
     gene            complement(7447..7761)
                     /gene="rplX"
                     /locus_tag="VFQ61_37205"
     CDS             complement(7447..7761)
                     /gene="rplX"
                     /locus_tag="VFQ61_37205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET9960206.1"
                     /translation="MMQRVRTGDTVLILSGEDKGKRGKVSKVLLDRGLVVVEGVNRVK
                     RHIRPTQQRAGGILEVEAPIDVSKVKLVDAASGKAGRVKFQIEDGKKVRVVKGASPAA
                     EK"
     gene            complement(7758..8126)
                     /gene="rplN"
                     /locus_tag="VFQ61_37210"
     CDS             complement(7758..8126)
                     /gene="rplN"
                     /locus_tag="VFQ61_37210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET9960207.1"
                     /translation="MIQMQTFLEVADNSGAKLVQCIKVLGGSRRRYAGLGDVIVVSIK
                     EALPGSKVKKGDTAKAVVVRTKREYRRNDGSYIKFDDNSAVLINAQQEPVGTRIFGPV
                     ARELRAKRFMKIISLAPEVL"
     gene            complement(8140..8385)
                     /gene="rpsQ"
                     /locus_tag="VFQ61_37215"
     CDS             complement(8140..8385)
                     /gene="rpsQ"
                     /locus_tag="VFQ61_37215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET9960208.1"
                     /translation="MIGRVASDKMDKTVVVEVVRFKRDSMYKKYVRVRKRYKAHDEKN
                     EFKVGDRVEIVEHRPLSREKRWAVVRLIERPASELTS"
     gene            complement(8424..8630)
                     /gene="rpmC"
                     /locus_tag="VFQ61_37220"
     CDS             complement(8424..8630)
                     /gene="rpmC"
                     /locus_tag="VFQ61_37220"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET9960209.1"
                     /translation="MKSKDLVERSSEDLATLRASMQKDLFSYRMKNWTDQLEDTSLLR
                     KTRRDLARIETILKQREQSQGGKP"
     gene            complement(8627..9040)
                     /gene="rplP"
                     /locus_tag="VFQ61_37225"
     CDS             complement(8627..9040)
                     /gene="rplP"
                     /locus_tag="VFQ61_37225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET9960210.1"
                     /translation="MLSPKRTKFRKQQKGKNRGVAWRGSDVSFGDFALQAVTHSHITS
                     RQIEAGRMAIQRHVKRAGKLWIRVFPDKPITKKPLEVRMGSGKGSVEGWVCVVKPGRV
                     LFEIAGVPEKDAREAFRLAASKLPIETKFLMRGAS"
     gene            complement(9047..9739)
                     /gene="rpsC"
                     /locus_tag="VFQ61_37230"
     CDS             complement(9047..9739)
                     /gene="rpsC"
                     /locus_tag="VFQ61_37230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET9960211.1"
                     /translation="MGQKTHPYGFRLGVIKTWTSKWYEDKQYQQWLHEDISIKRAVKD
                     YLYNANVASVEVERAANKAKVIVYTARPGMVIGKGGKGIEILKTGNLDSAQKGEARFP
                     GVQSFTQNEVFIDVQEVRKAETNAQLVAENIATQLERRIAFRRAMKKALTTAMKFGAK
                     GVRIRCAGRLGGAEMGRVETYREGRVPLHTLRADVDFGLAEARTTYGIIGVKVWIFKG
                     EVLQRKARRIPE"
     gene            complement(9998..10339)
                     /gene="rplV"
                     /locus_tag="VFQ61_37235"
     CDS             complement(9998..10339)
                     /gene="rplV"
                     /locus_tag="VFQ61_37235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET9960212.1"
                     /translation="MIANASARFQRISPRKARMIINFVRGRDASEALQLLDFTFKAAA
                     PFVKKLIASAVANAKQKNPNVDTESLFISKAAVDKGPNSQLRRWRPRAQGRATRIQKG
                     VSHITIELDAR"
     gene            10748..11272
                     /locus_tag="VFQ61_37240"
     CDS             10748..11272
                     /locus_tag="VFQ61_37240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET9960213.1"
                     /translation="MRGLINCSHAEHGEAVRAIFNDAIANTTAVYEYEPRSSERIRAW
                     FEDKANRGLPVLGAVDDRGSLLGFASYGAFRAWPAYKYSVEHSVYVHRDSRGRGLGRH
                     LLGALIEHARGAQLHLMVGGIDAENSASIALHERLGFQHAGTIRQAGFKFGRWLDLAF
                     YQLQLETPLQPVDG"
     gene            11688..12377
                     /locus_tag="VFQ61_37245"
     CDS             11688..12377
                     /locus_tag="VFQ61_37245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HET9960214.1"
                     /translation="MAETAVSATKPRLKLADTTTGQKALLAVSGVILFGFTIVHMLGN
                     LQVFLGPEQYNHYAEVLKGNPPLLWITRSVLLVSAVVHIVMVVKLYSRSLAARPVAYR
                     VKKNIATSYAASAMKYSGPALLFYILFHLAHFTFPGVAMGAYEHSPTDAYANFVNGFA
                     VPWVALLYVIANLFLGMHLFHGAFSMLQSIGANHPRYNDRARQVARFFALLVTAGNVI
                     LPLSVLTGLVQ"
     gene            12477..>13555
                     /gene="sdhA"
                     /locus_tag="VFQ61_37250"
     CDS             12477..>13555
                     /gene="sdhA"
                     /locus_tag="VFQ61_37250"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
                     /protein_id="HET9960215.1"
                     /translation="MELNSRAPSGPIEKRWSQRRFESKLVNPANRRKYKIIVVGSGLA
                     GGAAAASLGEMGYNVACFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSTYRLFYDTVK
                     GGDFRARESNVYRLAELSVNIIDQCVAQGVPFAREYSGLLANRSFGGAQVARTFYARG
                     QTGQQLLLGCYQALERQVEAGTVKMFPRTEMLDLIVVDGKARGIVARDLVTGKVTAHM
                     ADVVVLCTGGYGNVFYLSTNAKGCNATAAWRAHRRGALMANPCFTQIHPTCIPQAGSY
                     QSKLTLMSESLRNDGRVWVPKNPADCAKDPKSIPEGDRDYYLERLYPSFGNLVPRDIA
                     SRRAKDMCDQGRGVGPEVAGVRRGV"
BASE COUNT         2375 a         4357 c         4188 g         2635 t
ORIGIN      
        1 ttgctcgagc tgaatcgcct tgggacgaat cagatcacgc caattcctgc tcatcatcgg
       61 ggtaaccatg ctctacctcg tacagggtta gggtttgcgc ggccgtcccg ggcgagcttg
      121 ggaacccgct cccaaagctc gccccgcggc ctgcccgcag cattcgaaac agacacgttc
      181 agttagaaac cgaacgaagg cggggcgccg ctcgacggcc gcgccccgca gtcatcaacg
      241 cgagtagtac tcgaccacga gctgctcacg aaccatcggc tcgttcagct cgtcgcgtac
      301 cggcatcgac ttcacgacgc cgctgaagct gcccttgtcc acgtcgatcc agctgggacg
      361 ttggcggttc tccgccgctg ccagggccgc ttggatgaac gcgatcttgc ggctcttctc
      421 acgaacctcg atcttgtcct ccggcttgac ctggaagctc gggatgttga cgcgcttgcc
      481 gttcaccagg atgtggttgt ggcgcacgag ctgacgcccc tgcgcgcggc tgcctgccaa
      541 gcccatgcgg tggaccacgt tgtccaaacg acgctcgatc aggccgagca tctcttcacc
      601 ggaacgaccc gggaggtgcg tggccgcgac gtagtagctg gcgaactgcc gctcgagcag
      661 cccgtagatg cgacgcatct tttgcttctc gcgcaggcgc aggccgtact cgctcagctt
      721 gatgcgagcc tggccatgct gaccgggggg gtacgggcgg cgcccgaccg agcacttatc
      781 cgtgaagcag cgctcacctt tgaggtagag cttgccgcct tcacgccgac acagcttgca
      841 aaccggacca atataacgag ccatcctcta aatctccgtc acacgcgacg acgcttcggc
      901 ggtcggcagc cgttgtgggg aatgggcgtc acgtcgcgaa tcagcgtgac cttgaaacca
      961 acctgctgca gggcacgcag cgcgctctca cggccagcac ccggaccacg tacgaacaca
     1021 gccaccgagc gcaccccgtg ctccatggcg cggcgccccg cctcttcggc cgcgagctgc
     1081 gcggagaagg gcgtgctctt gcgcgaaccc ttgaaaccgc gcgaccccgc gctggcccaa
     1141 gagatcgtcg caccgttcac gtcggtgagc gtgacgatgg tgttgttgaa cgtcgactgg
     1201 atgtgggcga tgccggcgaa gatgttcttc ttcaccgtct ttttggctgg gcgcgctttg
     1261 ggagttgcca tgtgctgttc cgttcaaacc tgactcaggc gcgtcgcccg aggttcttgc
     1321 gcggaccctt gcgggtacgg gcgttcgttc gcgtacgctg ccctcggact ggaaggcctt
     1381 tgcggtgacg caggccgcgg tagcagccga gatccatcaa gcgcttgatg ttggcttgga
     1441 cgtcgcgccg agcgtcgccc tccaccttca cgttcgcttc gatcgcatcg cggatagcct
     1501 tgacgtcggc ctccgagagc tcttccacgc gacgagtcgc gggaatgttc gcgatgctgc
     1561 acaagcgcgc tgcggtcgca cgaccgatcc cgtaaatgta cgggagcgcg taaaggattt
     1621 gtttcctgcg ggggagatcg acgccggcga tgcgtgccat tggttttcct gattagccct
     1681 tgttttcggc tcagccctgc cgctgcttgt ggcgcgggtt gtcgcaaatg acgcgcagga
     1741 caccgcgacg tttcacgatt ttgcacttgt cgcagatctt tttgacgctc ggtcgcacct
     1801 tcatgtcctg cctcactgcc tctttatgat ttttccacgg ttcggatcgt aagccgcgac
     1861 ttcgacgatg acttgggctc ccacgagcaa gcgaactacc tgatggcgga actccgtcga
     1921 gatgccggcg ttgaccaacc gcccgctttc caagcgaacg cgatacaacg cattgggcaa
     1981 cacctcttcg actacgccct tctcagtcct gctcttttgg gatccggtct tcgtcctcag
     2041 cctccgatta actttccaaa gtgctcagaa tgcgctggct gatttccttc gggctgccca
     2101 cgccatctac ccggcgaagt agcccggcgg cggagtagaa cggcaccaat gctgcggtca
     2161 ccgcgtcgta ctgtgccagc cgcttgatga ccgtcgcctc ctgatcgtcg gcccgatgtt
     2221 cgagctcggc gtctgggggg ggtgggttat acttcaggtg gtagatctgt ccagtccgtt
     2281 tatcggtccg gcgcaaggtg gcgcgctcta ccagaagctc gtgggccacg tcgatcaaaa
     2341 tcaccgcgtc cagcttcgtt ccgagcttgg tcaacaagcc ggagagcgcc tcggcctgcg
     2401 gtacgctgcg cgggtagccg tccagcacga agcctttagc cgcgtcgggt gccagaaagc
     2461 gctgctctac catgcggatc accagatcgt ccgggacgag cttgccttcc gacatgtagt
     2521 tttttgcttc gagcccgagc gcggttccct gcgcgaggtg ctctcggagc atgtcccccg
     2581 tcgaaatctt tgggaccgaa aagcgctcgc aaagaacgat agcttgggtg cctttgccgc
     2641 tcgcgggggc accgagaaga atgagcctca tgttgtcctc cggccgctga gtcgtgtcat
     2701 tcgcggacct gtgaggcctt catagctgcg ggtaatcaag tgcgcttcga tttgattgac
     2761 cgtctcgagg gccacaccga cgacgatcat caagctggtg ccgccgaact ggaagggcac
     2821 gcggaaggac gtcgcgatca ctgacgggat cacgcagatg gccgcgacgt agatcgatcc
     2881 agcgaccgtc aggcgcgtca gcacgcgatc gatgtactcg gctgtctgct tgccggggcg
     2941 tacaccggga atgttggcct gttgcttctt caggttctcc gccacatcca ccggctggaa
     3001 cgtgaccgcc gtgtagaaga aggagaagaa gatgcaaagg gaggcgaaca cggtctggaa
     3061 agcccagtca ccgcggttca gcacgccgct cagacgctgc atgccgggga tgttgaagtt
     3121 ggccaaagtg gccgggaaca tcagcaagct ggaggcaaag atggccggga tcatgctggc
     3181 catgttcacc ttcagaggca ggtgggcgcg ttgggccccg tacacgtggc gaccgacttg
     3241 gcgccgcgag tactggatgg gaatgcgccg ctgggcgcgc tcgaagaaca ccacacaggc
     3301 cacgctcatc acgagcaccg cgaccagaat ggtgatcgtg agcggctgga tgtcgccggt
     3361 gttgcccgcg atgtagttca tgatgccgcc aggaatgccg ctcacgatgc tggcgaagat
     3421 gatcatcgac gtgccgttgc cgatgccgcg ctccgtcgct tgctcgccga gccacatcag
     3481 cagacccgtg ccggtcgtga gcgtaatcac ggtcatcagc cggaagcccc aacccggatc
     3541 ggcaaccacg tcgccgaagc gaccgccgcc catgtccgag ttgttcatgc cttccaacat
     3601 gctcgccacg ccgaagccct ggaacaagct cagcacgatc gtgccgtagc gcgtccactg
     3661 ctcgagcttg cggcggcccg cttcaccttc gcgacgcatc tcctcgactt gcttgctgac
     3721 caagccgagc agctgcatca cgatgctggc cgagacgtac ggcatgatgc cgagcgcgaa
     3781 gatcgagagg ttctgcaggg caccgccaga gaacaggttg acgaacccga gtaggccgcc
     3841 ctgagtcttg acgatgttgc gcatcaccga gcgatcgacg ccgggtgtcg tgatgaacac
     3901 cccaacgcga tagatcgcga ccacgccgag cgtgaacagg atgcgctgcc ggagctccgg
     3961 caccttcccg atgttggaga agccttgcag aaccgccatc ctgttcccga cgctcgagcg
     4021 cgcgagcgct cagccctgac cttccgaggc ttcggttgct tccgtagccg gcccgaatgc
     4081 gagcacggtc gcggtcccac cggccttctc gatcttctcg agcgccgact tgctgaaggc
     4141 gtgcgcgctg atggtgagct tcttggagag atccccgtca ccgagaatct tgatccgaac
     4201 accgcgcccc tgaaccaacc gagcgtcacg cagcgctgcc tcatcgacca cgctaccttc
     4261 agcgaaatct tcgagcacac cgatgttcac gccgacggtc ttgatcggaa acggcacgcg
     4321 gaagccgcgc ttcggcagac gacgctgcag cggcgtctga ccaccctgga agtggagctt
     4381 accgaagtca ccgccgtggc gagctttctg gcctttcgag cccttgccac aggtcttgcc
     4441 caggccagag ccgataccgc gccctaggcg cttctcggat ttgcgagcgc cttcggggta
     4501 tgcgaggcgg gagagatccg tactcatgac tgtttctcct cgaccttaac gaggtgcaga
     4561 accttcttga tcatgccgcg gaacgagggc gtgttgtcga ccagcacgct cgaaccgggg
     4621 ccgcgcaagc cgaggccgcg cagcacgcgc cgttgcgggt gcggacgacc aatctcgctg
     4681 tgcacttgtc gtacttcgag cttggttgcc atcacgtcga ccctcccgca cccgagcggg
     4741 tgttggcgac gatcgaggca gccgtagcgg cctgggccga ggacgccgcg accgggtaat
     4801 ccccagccac gtgatccatg ccgagcccgc gcgactgcgc gatcttttcg gccgagcgca
     4861 gacgctgcag cgccgcgacc gtggcgtgaa ccacgttgta cttgttggaa gacccgaggc
     4921 acttgctcaa gatgtcgtgg ataccggccg actcgaccac ggcgcgcacc ggaccgccgg
     4981 cgataacgcc cgtaccaggc gaagcggggc ggagcagcac cttaccggag ccgaactccc
     5041 ccaccacgtc gtgaggcacg gtcacgccga ccatggggac cttgaacatg ttcttgcggg
     5101 cttgatcgtt gcccttgcgg atggcttccg gaacctcgtt ggctttgccg agacccacgc
     5161 ccacgtggcc gttctcgtca cccacgacga ccagagccga aaagctgaag cgccggccgc
     5221 ccttgacgac cttggccaca cggttgatgt ggatcacgcg ctctttgagc ttttcctcgt
     5281 cgatatgttc gatgctcatc agagtgtcct tacagggctc agaagttgag tccgccctcg
     5341 cgagcggcct cggcaagcgc cttgacgcgg ccgtggtaga ggtaaccgtt gcggtcgaaa
     5401 acgacctttt cgacgttctt cgcgaggcac atcttggcga tcagcgcgcc gaccttacgg
     5461 gcggcgtcac tcttcgcgtc ctcggtcagg gtgcctttga gatcgcgcga cagcgtggag
     5521 gcggaggcca ccgtgcggcc gtcggtatcg atcacgacct gcgcgtagat atgccggccc
     5581 gagcggaaca ccgacaagcg cggacgttcc tcggttccgg tgatgcggct tcgaattctg
     5641 agtttccggc gctcacgacc ggtcagttta gctgccattg ccttactccg agctcgcgct
     5701 cacttaccct tgcccttggc ggccttaccc gctttgcggc ggatggtttc gccgcggtaa
     5761 cgaatgccct tgccaccata cggctctggt ggacgcaggt cgcggatcgt ggccgcgatt
     5821 tgcccgagcg ccgccttgtc ggcggagttc agcaccaaca tggtgccctt ggagtccggc
     5881 ggtacgttgc cggttacgcc agcgggcaac tggatttgcg tggggtgtga gaagccgagg
     5941 ctcagcgtaa cgacctggcc cttgacctca gcgcggtaac cggttccgac cagctcgagc
     6001 tgtcgctcgt agccttcggc ggcacctttc acgcggctcg cgagtagcgc gcgcgccagg
     6061 ccctgcaggc gagctgcgtc acgaccctcg gcgtcgctct cgagcagcac cttgtcgccg
     6121 tctttcttga cggagacatt cggcggcaag tccatcgcga gcttgccctt ggggccctgg
     6181 atctcgaggc gggttcccgc cacgttgacg gcaacgccct tcggcagaac cacgggacgc
     6241 ttaccgacgc gcgaaaggcg cttcggcgcg gcttgttggg tttgttcgga catcaccaaa
     6301 cctcgcagag taactcacca ccgatcttgc gctgacgcgc ttctcggtcg gtcatcaagc
     6361 cgtgcgacgt gctcaggatc gaaaccccga gaccgctcaa cacgcgcgga atgtccgtgt
     6421 gacggacgta gacgcggcgg cccgggcgag agacccgacg caggccttga aaggcgctct
     6481 cacggttgtt gtacttgagc acgatcgtca gggtggagtg cttcttgggc ccccactctt
     6541 cctgtcgcac gtcagccacg taaccctcgg ccttgaggat ctccgcgatg cccttcttca
     6601 acccgctcgc cggcaagcgc gtgagctcgt gacgagccaa cgcggcattg cggatgcggg
     6661 ccaacatatc ggaaacggga tcgttgaccg tcgccatcac cagctcgctt tcgtaacgcc
     6721 gggcagctca ccgcgcagcg ccattgagcg caagcacagg cggcagaggt tgaagtcgcg
     6781 atagaacgcg cgagagcgac cgcagacgcg gcagcgactc acggcgcggg tggaaaactt
     6841 cgggggtcgg ttcaacttgg cccactgaga agcgcgtgcc atcggttcag ctcctgaacg
     6901 gcatgcccaa cgacccgagg agtgctttgg cttcctcgtc ggtctcggcg ctggttacga
     6961 aggtgatgtt catgcccttg attttctcga ccttgtcgaa gtcgatttcc gggaagacga
     7021 tctgttcacg gagacccagc gtgtagttgc cagctccgtc gaagccgcgc gggctgatgc
     7081 cccggaagtc acgcacgcgg ggcagcgcga gcgtcaccag gcgatccaga aactcccaca
     7141 tacgttcgcg gcgcagggtg accttgacgc cgatcggcat gttttcgcgc aacttgaagg
     7201 tcgcgatcga cttcttggcg cgcgtcacca ccggcttctg accggtgatg gaggtgacct
     7261 cggccaccgc ggcgtcgatc agcttcggat tggcaaccgc ctcaccgagg cccatgttga
     7321 gcacgatcgc cttgaggcgg ggcacctgca ttggatttcc gtacccgaat tgcttcatca
     7381 ggcccggaac gacgtctttc ttgtatcgct cgcgcatgcg aggctcgacc acgtcggtca
     7441 tttccgtcac ttctccgctg cggggctagc gcccttgacg acgcgcactt tcttgccgtc
     7501 ttcgatctgg aacttgacgc gaccggcctt acccgatgct gcgtcgacca gtttgacctt
     7561 gctcacgtcg atcggcgcct cgacctcgag gatgccgccg gcgcgctgtt gggtggggcg
     7621 gatgtgccgc ttcacccggt tcacgccctc cacgaccacc aggccgcggt cgagcagaac
     7681 cttggagact ttgccgcgct tgcccttgtc ctcaccgctg aggatcagga ccgtatcgcc
     7741 ggttcgcacg cgttgcatca caacacctcc ggtgcgagcg agatgatctt catgaagcgc
     7801 ttggcgcgca gctcacgggc gacgggccca aagatacgag ttccaaccgg ctcctgctga
     7861 gcgttgatca gcacggcgct gttgtcgtcg aacttgatgt agctgccgtc gttgcgtcgg
     7921 tactcgcgct tggtgcgaac gacgacggcc ttggccgtat cgcccttctt caccttgctg
     7981 cccggcaggg cttctttgat gctgacgacg atgacgtcgc ccaggcccgc gtaacggcgg
     8041 cgcgatccgc ccagcacctt gatgcactgc acgagcttgg cgccggagtt gtcggccact
     8101 tccagaaagg tctgcatctg aatcatgttc ggtgctcctt caggaagtga gttcagaagc
     8161 ggggcgttcg atcaggcgaa cgacggccca gcgcttctcc cgggaaagcg gacgatgctc
     8221 cacgatttcg acgcggtcac cgaccttgaa ttcgttcttc tcgtcgtggg ccttgtagcg
     8281 cttccgcacc cgcacgtact tcttgtacat ggagtcgcgt ttgaagcgga cgacctcgac
     8341 gacgacggtc ttgtccatct tgtcgctcgc cacgcgcccg atcagcttgc ggcgatagac
     8401 ggcctcttgg tgggcttcgg agctcacggc ttgcctcctt ggctctgctc gcgctgcttc
     8461 aagatggtct cgatgcgcgc gagatcgcgc cgggtcttgc ggagcaagct cgtgtcctcg
     8521 agctgatccg tccaattctt catacggtaa gaaaagaggt ccttctgcat cgacgcacgc
     8581 agcgtcgcga ggtcctccga tgaacgctcg actagatctt tggacttcac gacgcacctc
     8641 gcatcaggaa cttggtctcg atggggagct tcgaagccgc caagcggaac gcttcacggg
     8701 cgtccttctc gggcacgccg gcgatctcga agagaacgcg gccgggcttc acgacgcaga
     8761 cccaaccttc cacgctgccc ttaccgctac ccatgcggac ttcgagcggc ttcttggtga
     8821 tcggcttgtc ggggaacacg cggatccaga gtttgcccgc gcgcttcacg tggcgctgaa
     8881 tcgccatacg gccggcttcg atctggcgcg aggtgatgtg actgtgcgtc acggcctgca
     8941 acgcgaagtc accgaaggac acgtccgagc cgcgccaagc gactccgcgg ttcttgccct
     9001 tctgctgctt tctgaatttg gtgcgtttcg gggagagcat taccaatcac tccgggatgc
     9061 ggcgcgcctt acgctggagc acctcgccct tgaagatcca gaccttgacg ccgatgatgc
     9121 cgtacgtcgt gcgggcttct gcgagaccga agtccacgtc cgcgcgcaac gtgtgcagcg
     9181 gaacgcgacc ttcgcggtag gtttcgacac gacccatttc agcgccgccg aggcgacccg
     9241 cgcagcggat gcgcacgccc ttcgcgccga acttcatggc cgtggtcagc gccttcttca
     9301 tggcgcgacg gaatgcgatg cggcgctcga gctgggtcgc gatgttctcc gcgacgagct
     9361 gggcgttggt ctccgccttg cgcacctcct ggacgtcgat gaacacctcg ttctgcgtga
     9421 acgactgcac acccgggaag cgggcctcgc ccttttgggc cgagtccagg ttgccggtct
     9481 tcaggatctc gatgcccttg ccgcccttgc cgatcaccat acccgggcga gccgtgtaca
     9541 cgatgacctt cgccttgttg gcggcgcgct cgacctcaac gctcgcgacg ttcgcgttgt
     9601 agaggtagtc cttcacggcg cgcttgatgc tgatgtcctc gtgaagccac tgctggtact
     9661 gcttgtcctc gtaccacttg ctggtccacg tcttgatgac gccgaggcga aagccataag
     9721 ggtgtgtttt ttgtcccatc ttgattccgt gaagtcgcga atccgtgaaa atcgcgcttg
     9781 aaacttgcgg gtcgtgaact tgacgtgcgg cgctgagcta ccctcccccg gcgtagaacg
     9841 acggttggag agagattcgg cggcgcctca gggagcccgg cagtgccccg tagcggagca
     9901 aattccgggg ctagacccag aaacgaggag cttggaacgc ggtgcggagc ggtacccagc
     9961 gagaccggcc ggtggccaat ccccgccgag tcaaggctca gcgcgcgtcg agctcgatcg
    10021 tgatgtgact cacgcctttc tggatgcggg ttgctcggcc ttgagcacga gggcgccagc
    10081 gacgcagctg cgagttcggc cccttgtcga ccgcggcctt gctgatgaac agcgactcgg
    10141 tgtcgacgtt ggggttcttt tgcttggcgt tcgccacggc gctcgcgatc aacttcttga
    10201 cgaagggagc tgccgccttg aacgtgaagt cgagaagctg caaagcttcc gaggcatcac
    10261 gcccgcgaac gaaattgatg atcatccgag ctttgcgcgg actgatcctc tgaaaacgag
    10321 cactagcatt agcgatcatg gcgacagttt cctcgtgaga cccgtcgacc cctggggcgt
    10381 caaatgtcgg cgccccgaca tccgccacca gaggcggctg gcagcgcccg ccacccggtt
    10441 gggagtgggc ctgcaagggg accggccttc tagcaaagca cccccgggta ggcaagcccg
    10501 tcctacatgg aagtactcgg cggccgttaa ttggctgctc ggacagcgct ggggcgtgga
    10561 cttgctggag actgaaccag gcccggagcc ggtgtgggtc cctggtatat ccccagtcca
    10621 gccggacttc taataccagg cggggacgaa tcgtcccgtt cggatcggtc gggctggctt
    10681 acggtcacgt ggggcgcccg gaagcggtcg acgtgatggc gggaggcgat gtccaatgga
    10741 gcgaagcatg cgaggtctga tcaactgttc acacgcggag catggcgaag ccgtgcgcgc
    10801 catcttcaac gacgcaatcg cgaacactac ggccgtgtac gagtacgagc ctcgatcgag
    10861 tgagcggatt cgagcttggt tcgaggacaa ggccaaccgt ggcctgcccg ttctgggggc
    10921 ggtggacgat cgaggtagtc ttttgggctt tgcgagctac ggcgcgtttc gcgcctggcc
    10981 ggcatataag tacagcgtcg agcactccgt ttatgtccac cgagacagtc gaggtcgcgg
    11041 actggggcgt cacttgctcg gcgcactgat cgagcatgcg cgtggcgcgc agctgcacct
    11101 catggtgggt ggaatcgacg ccgaaaatag cgcgagcatc gcgttgcacg agcgactcgg
    11161 gtttcagcac gctggcacga tccgacaggc tggcttcaag ttcggacgct ggctggacct
    11221 tgcgttctat cagctacagc tagaaacccc gctccagccc gtcgacggct aaaggatttt
    11281 gggtcagtag gagggccaca cagtcgtgct ttttccggcc ctgacggcct acattttcgt
    11341 cggctctgcg tccgggaaaa gacttgacga atccgccagt taaacctatg cagcccgggt
    11401 ccaggaccac gtctttgcga cccgaacctg gtatttgcgg cacaacccga gctcgcactg
    11461 cgagctcgga tgagtgccac gcgagcagtg ctgacggcat tggacgctcg gcattgtttc
    11521 gtggcgactc tcgaacgccc cgcggtctgg atagactgtg gcgcgcgaaa gcccgggccg
    11581 cgaaggtcgc gcccatgttt cagaattttt acggcgagcg ccgctgtgcg cccgcacacg
    11641 aggcctcgcc ttcgtcacgc gcctcgacag accttggaga ccgatagatg gccgaaactg
    11701 cagtgagcgc aacgaaacct cggctcaagc tggccgacac cacgacggga cagaaagcgt
    11761 tgctggccgt cagcggcgtg atcttgttcg gtttcacaat agttcacatg ctcggaaacc
    11821 tgcaggtttt cctggggccc gagcaataca accactacgc ggaggtgctg aagggcaatc
    11881 cgccgctgct ttggatcacg cgcagtgtgt tgctggtttc ggccgtcgtc cacatcgtga
    11941 tggtggtcaa gctctactca cggtcgctcg ccgcgaggcc ggtggcgtac cgtgtgaaga
    12001 agaacatagc caccagctac gcggcctccg ccatgaagta cagcgggccc gccctgctgt
    12061 tctacatcct cttccacttg gctcacttca ccttcccggg tgtggccatg ggagcttacg
    12121 agcacagccc aacggacgcc tacgcaaact tcgtaaatgg cttcgcggtt ccctgggtcg
    12181 cgctgctgta cgtgatcgcg aacctgttcc tcggcatgca cctgttccac ggcgccttca
    12241 gcatgctgca gtcgattggc gccaatcacc cccgttacaa cgaccgcgcg cgtcaggtcg
    12301 cgcgcttctt cgcgctgctc gtcacggccg gcaacgtcat tctgccgctc tccgtcctga
    12361 cgggcctagt ccaatagctg tccaatagct gcgcaatagc ccgtgcaata gccaggttct
    12421 gcgaaccggg ctaaccaccg gcacacgcac acggaagaac tcttcggaaa gctgccatgg
    12481 aactcaactc tcgcgctcct tcgggcccca tcgagaaacg ttggagccag cgtcgtttcg
    12541 aaagcaagct ggtcaacccc gccaaccgcc gtaagtacaa gatcatcgtc gtcggctccg
    12601 gcttggcggg cggcgcggcg gccgcaagcc tcggcgaaat gggctacaac gtggcctgct
    12661 tctgctacca ggacagtcca cgccgcgcgc actcgatcgc cgcgcaaggt ggcatcaacg
    12721 ccgcaaagaa ctaccagaac gacggcgaca gcacttaccg cctgttctac gacacggtga
    12781 agggcggcga cttccgcgcg cgcgagtcca atgtttatcg gctcgcggag ctgtcggtga
    12841 acatcatcga tcagtgcgtc gcgcaaggcg tgccgttcgc gcgtgagtac agcgggttgc
    12901 tcgccaaccg ctcgttcggt ggtgcgcaag tagcccgcac gttctacgcg cgcggccaga
    12961 ccggacagca gctgctgctc ggctgctatc aggcactgga gcgccaggtc gaagcgggca
    13021 cggtcaagat gttcccgcgc accgagatgc tcgatctgat cgtggtcgac ggcaaagcgc
    13081 gcggcatcgt ggcgcgcgac ctggtgacgg gcaaagtcac cgcgcacatg gcggacgtgg
    13141 tcgtgttgtg caccggcggc tacggcaacg tcttctatct ctccaccaac gccaaaggct
    13201 gcaacgccac cgcggcctgg cgcgcccacc gtcgcggcgc cttgatggcg aatccctgct
    13261 tcacgcagat tcaccccacc tgcatcccgc aagcgggttc gtatcagtcg aagctcaccc
    13321 taatgagtga gtcactgcgc aacgacgggc gcgtgtgggt gcccaagaat cctgccgact
    13381 gcgccaagga tcccaagagc atccccgagg gtgaccgcga ctactacttg gagcgcttgt
    13441 acccgagctt cggaaacctg gtgccgcgcg atatcgcctc gcgtcgcgcc aaagacatgt
    13501 gcgatcaagg gcgcggcgtg ggccccgagg tagccggcgt gcggcgcggc gttta
//
LOCUS       DASTKU010001050        28844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1770250, whole genome shotgun sequence.
ACCESSION   DASTKU010001050 DASTKU010000000
VERSION     DASTKU010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 28844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28844
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770250"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFQ61_37255"
     CDS             <1..585
                     /locus_tag="VFQ61_37255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HET9960216.1"
                     /translation="FVEFPCPPDLVGLNYYVTSERFLDSRVELYPPAMVGGNGRHAYA
                     DVEAVRVCADGLVGPTCFLVDAYERYDKPVAITEAHLGCSPEQQIRWLAYVWNSAQTA
                     HALGVDVRAVTAWALFGAYGWDRLVTEGPGRYEPGVFDVSPGFPIETPLSDFVRALAS
                     GEFSPLAPGWWEVEARLLYEPHVAPKPERKSRAA"
     gene            615..1826
                     /locus_tag="VFQ61_37260"
     CDS             615..1826
                     /locus_tag="VFQ61_37260"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET9960217.1"
                     /translation="MANSILDISQPRSSSPTASNMRRRELLLGVVTGGFAMTSLGCSR
                     NASSGSNSNAAGPSQGPQGNTPPGTGKTNPRQLGVALLGLGNYSETQLAPSLKLTRHC
                     SLRGIVTGSPDKIPRWQKEYGIPDRNVYDYGSLPRIADNSAIDIVYIVVPTALHAKYA
                     VQAAEAGKHVWCEKPMAMTVEECQKIIDACRANHVSLAIGYRMHHEPNTQTVMQFARE
                     KPYGAIKAVRAIAGDRSNGENTWRMVRAMGGGALYDMGVYSINAIRYASGEEPVRVVR
                     ARQWADRPELFRDVDENTEFELELPSGAIAYGKASRFDKTNQLRVEAQRGWYQLEPMQ
                     AYEGVQGETSDGKKLDKKVDKQQARQMDDEALALLEGRPAMVPGEEGQRDIRLVQAIL
                     QAASTDKPVAL"
     gene            complement(1853..2578)
                     /locus_tag="VFQ61_37265"
     CDS             complement(1853..2578)
                     /locus_tag="VFQ61_37265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960218.1"
                     /translation="MSNIQSVCDEMKGLEGTVGAFVWQGGRCLASSLPKAYDSARLNQ
                     IGSGLSRLGQLSDKAGYDRSACVFHWPRATLFSWPLPNAGVLAIVASPKVARGELELS
                     VAIAMEELGVLLGQDVSAGSQDASTVPSMISAPVIVEKPSASFAPTAAAAPVISASVE
                     PPIDDRASAYRLEQIESLLTSEVGPNGRAMLQRLRRQAAQAGIAEAAWLPRLRTLLLS
                     ELKDPCARAVVAASILFVPGAEH"
     gene            complement(2575..3387)
                     /locus_tag="VFQ61_37270"
     CDS             complement(2575..3387)
                     /locus_tag="VFQ61_37270"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9960219.1"
                     /translation="MATQTQTAAINDRTPDRSAEPASGSTTLRGRIEAIGIHDLLRVS
                     VSKGSSGRLLVFNEQSDSELYYEHGRLMAVTSGSEHGPECLEKVLGMTEGEFEFAWGL
                     VAEPERRTADLHERMLAAVKEFYERQVRERAKSRPEGRLNSGVHKVAAPAESSPSDAA
                     RNEATAEPAAAAAAGEEVPVGELGRATLDAAGRVIAREGTFTQYDAGLAALTLKVAAA
                     IGSALAVKDLQGFEVHAAGGQGLVCLVNSTGMRAVKTSVDMDIDVLRKRLEK"
     gene            complement(3755..4432)
                     /locus_tag="VFQ61_37275"
     CDS             complement(3755..4432)
                     /locus_tag="VFQ61_37275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960220.1"
                     /translation="MSNRWKTWLPFAAVCGTTLIAASGSAEGAREGAREKNAAARVEE
                     PAAPRAEDRSAQDQEILRIANDLASEAGAVMERWLASDALKEERLLSRLYYPIRNTNP
                     VKYNTDYDALADRDLQPLLDKHATRNAAIQYTVVTDLNGYVPTHNRQFSQPLTGNRAV
                     DLVNNRSKRIFGDTVGFNASRNETPFLIQPYSRDTSENLFDLSVPLRIRGKHFGCVRI
                     GYKRVAP"
     gene            complement(4460..5374)
                     /locus_tag="VFQ61_37280"
     CDS             complement(4460..5374)
                     /locus_tag="VFQ61_37280"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET9960221.1"
                     /translation="MVFAAIRNSISLKVSLTLAAVTLALTSISAGVIISHQTAAMEDM
                     TLSKAKLSAQLGAQGYGQLLEQAVDDGHVTVQDVFDTNYEEIKGYDWGGKPKYHTRYD
                     FITDRIAVGFLDRFLESDDFVAAIAGDVNGYVPTHNSKFMKPLTGDPVKDLEGNRTKR
                     IFNDDIAMRAAKSVDPVLIQPYRRDTGAMLWYVSSPVFVKGKHWGAFRVLVSMDEIAR
                     KRGSLIGTLTGMFAVFAVASAGVIFLMIRRSMQPLTALTQIADQLSIGEGLETPIKPA
                     TTDEVGTMAKSLDRLRASLKAAMARLGE"
     gene            complement(5411..6205)
                     /locus_tag="VFQ61_37285"
     CDS             complement(5411..6205)
                     /locus_tag="VFQ61_37285"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET9960222.1"
                     /translation="MENILKTLNELEGVHGTILADSNGRVIAYQAHSIYDGDLLSQAS
                     RAIASALDSVKLLHEDWESVTTQFAEGKLLVRNVTGTKPASSPALCLVLVADTRLNPS
                     FAGVAIRVAVAKLRALAEAAGGVANLANARGTLGAVGTSSAPATPVSHAPSQANPAAP
                     FKTQVAEVATSGLSWSGFGSSALASSGVTVLDPASSAALTACTKALARNVGPMAKVFV
                     KEAVRKVCGDRPFSKDQTSALVAELSKSIEDPSDAAQFRKTALRVI"
     gene            complement(6216..6533)
                     /locus_tag="VFQ61_37290"
     CDS             complement(6216..6533)
                     /locus_tag="VFQ61_37290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET9960223.1"
                     /translation="MKDFNVIAKFPEVQGAVLSDPSGALLDVAGNIDGEVAGAVHAYS
                     MKSLGQAGELLGLGGIQRTAIVGPTKSVVITIHDQEILGVYVDPDKPLAAIEKKLQDT
                     PKR"
     gene            7072..8538
                     /locus_tag="VFQ61_37295"
     CDS             7072..8538
                     /locus_tag="VFQ61_37295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960224.1"
                     /translation="MSASQLVSKIPGVRRCIVTDGVGHVLEGPAEERSTAEVSRAAVG
                     LFHRLCEGGALLGLGQPQLVTVRAAEGSRVVGFRGEASISVEVDPKLPRSIGQVESTL
                     LRNDWTAEGDWLIDESDIECLAPEGETAAPPWSPSNADVGWTELRSALARSDAGRVRL
                     QRPRRSAARPSGLQSAGLGIPGFESLIDALTKAVESIDAGDNHAGLEALALLTVELRD
                     TPPASTSLRWLTELWSARAILHAGEALDAADAHAQAALAEAGELDLRARALSTLLIAE
                     VALYRRHLNRCLELVRDARELFTDLKDDHELASCWLLEARALAASVRDNESLRAAEQA
                     HHHRPSWPPPLTFLVKRALCAGRVSDAEQALEPMLARETPPLEVTAHRCLLDHVKAGT
                     IASHTAYEYLRLLDSPPTRGTLRQLEALVRAFPTVEQFQETLGCKLLHAGHYESAALA
                     LERLAVKRGAPSEQLQAALTLARACLGRTHRSSQADHD"
     gene            8531..10588
                     /locus_tag="VFQ61_37300"
     CDS             8531..10588
                     /locus_tag="VFQ61_37300"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9960225.1"
                     /translation="MTDAARLTGAKLGNYRLERMLGRGRMGVVYLARDEALLRPTAVK
                     ILSWEIEDHEGQDPQAWFLAEARNVARINHPHVVQIYGVAKHPPHCYIAMEYVPGMGA
                     DSWMQDHGPFPARRATEILLQTALALQAAHDAGVVHRDIKPGNLLITGDGAVKLGDFG
                     MALHVASAHAGGRVRAGTPFYTAPEIWCGQTAAPASDLYALGATYYHLLTGNPPFQAA
                     NLETLIAAHLNAPLPDHPLLADTPEACWELLRRCLAKSPKERFESAQALSWEARGLLK
                     ALNGSASSSQPLSHTMPAAACSGPARTPDAHEVGATPKASRIQHAGDARNILDALKID
                     PTPKVDPAPKINAPLVAKAAVEQGWWVGLGFDRKPFSELELGEAPYPGEPFAGLWVEL
                     LHAFDDAPGEQDSVPARSPRGPLAGNTAGTVLLSGRSGSGRTTLVRRLATEHEPFAPV
                     AYVDLARELEPNLTASRKTWALIRRSFGLLSVPSPNSRELDSLLDQLGHLRPDQSAPA
                     LLILDGFEPRHFSRADLALLLRAARATRALRIVLVGGPDLVAQMRECDLWEPRCLAGI
                     LTVPPLSAGQTLRYTTDWLRATRAPDRNVILFTPDASLILAHRGEGNLALINRGATSM
                     LRIAQEENRRVLSSWHAWVAAGGSGEALSASPSPPRPWPSAEVLEIINRGRRDAGLPV
                     RSR"
     gene            complement(10595..11908)
                     /locus_tag="VFQ61_37305"
     CDS             complement(10595..11908)
                     /locus_tag="VFQ61_37305"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9960226.1"
                     /translation="MKQASFGRRDLLRAAGLTLLVPSFLREAFAEPEKSGPRLLIFMQ
                     ANGTHQATFWPDPNTGSSPILEPILGEPSLAERTLLIRGVNNLTTGPGNEHDRGFSSL
                     WTGVPTIGKPDDAFGGGPSIDQILKAALAPKVLFPTLNVGVLAADVAPKNGHRRSFSY
                     IGPRQQIPTQVDPYRVYASLFRTSELEPAQARQQLQLRRSVLDASARDLAALAARLGP
                     NQRQKLDAHTTALREYEARLTASLEGQSNCAKPKAPEAGLDPSLEDNVPRLLELMLDA
                     VAVALACNLTRIVTFPIGMSGNQWFYRWLGINIDNHSDVAHLDADDGSNPAIAEIMTQ
                     ISRWVAMHVAKLAQQLQAIPEEDGSVLDHSLVVWANENATGFHRLDNVPVVMLGRAGG
                     RLTRSGVFYEGAQSHHQLCTTVLQLMGVQVAGYGDQPGCGPLTGF"
     gene            complement(11905..13656)
                     /locus_tag="VFQ61_37310"
     CDS             complement(11905..13656)
                     /locus_tag="VFQ61_37310"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1588 domain-containing protein"
                     /protein_id="HET9960227.1"
                     /translation="MFSSGGIGGLVIGLVMVATGCSGPEGEPGAAITDPSPAAGGSSA
                     VRSSDGGANTSSSGGAPDSTSSASGGSSSTGCEPVTERRVRRLTAREYVQSVIALTGA
                     EPVAPSLPDPIVHGFDNNADALNITAGNFDEYAIAAELSAANLDVDRLAPCGDEGDAP
                     RDCARAFAQPFASLAYGRDISETELESLLALYDTGNRAGGYATGIRLIAESVLLSPFF
                     LYRTELGVEGSTATAELTPEEKVNALSFALTGARPDAELVAWVQAPERLSQREEVEKQ
                     ARRLVTSDAGRQHLAHFVRGLFGVLDLRLVNKEPVRFPIYTPAVKADLDREIELFLER
                     ALGPGGGTWEAVLGADTTFMNQNLFRVIYAADYAPAEAPRLPSTGEFVAVPLNTRIRR
                     GLLGLAGWEAAHSPIHRSSPVDRAVNVRSRLLCAPIPPPVGLMFTSPPPGDETGTTRQ
                     KFEVHTRDQACQACHRLIDPLGFGLEMIDALGRYREQENNLPVDSSGALVGTDVDGPF
                     SGPAELSLLLLKSQQARECVAIQWFRFSEGREEESADACVLEPLKKFFARGSRSFADL
                     AVETALQRNFMRRRREP"
     gene            14205..16685
                     /locus_tag="VFQ61_37315"
     CDS             14205..16685
                     /locus_tag="VFQ61_37315"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HET9960228.1"
                     /translation="MLNKSRYGVRRPDRSQFRSRWSAPLGLALLSLACSSGANGSGRP
                     HSTRPGQGAAGSASEGPGGWTSLGQGGTNPLLPVSTAGVGALSNGGVSGTAGTTSEVP
                     SFYCIPEPFPKDYSCQGSEGPWAEALQKALWFFNVQKSGPGVYCTDVQWRGDAHVGDG
                     HIKLDPSDPNGVNLPQAFITQHRTELDPDGNGEVDLAGGYHDAGDYIKFTMTTTYAAS
                     MIAWSMHEYPDAYRKTSLAGEALGQIRWAADYLMKATFRDSAGKLVAYAHQVADASDH
                     SCFWMPPEVRRPELCPRKAYFVWDGQPAADVTASAAAALALTAIVTRENSTSPADLAY
                     AERCLDYAKSLYEFAARYPMTRESDDGGLYVSNSSTDDVAWAAIWLYLSDPEHNRAYL
                     DATISGKQPWLSFGSQSNLLIPDPELGYRPAWAESSPHSWDAVRAGVIMKLAQITTEL
                     GDKTATDWQNIARQMAQGFATGGSTPDGFYMYMSWGSARYNSAAQFTSLLYSKYFPND
                     PKSAEFSSWAQRQVDHLLGANNLGLSYIMGYTDSYPLQPHHAAGHASIYGLPAKPAEN
                     RHIIWGALVNGPNGKGEHVDARDDYGSNEVTIDYNGALVAALAGHLATRGANECPLKV
                     FPPLEPRIDEFYAMGNLNIAPGGCRTQVNVRTMNESIHPPRYDETLSTRFWFDASEVR
                     AAGLDPSQAVTLNVFRDTGQNATPPEPTGVKGPIQCKEHTDTYYFEFDYTGAKFWGLI
                     PAIGAPRDLLLDYGLAYGEKKCEWDPSNDWSMQDLKVGQTEEVAKTPYITVYSKGRLI
                     WGKEPPCDGIPDVPVVPPPPPPLEYPPR"
     gene            complement(16693..18828)
                     /locus_tag="VFQ61_37320"
     CDS             complement(16693..18828)
                     /locus_tag="VFQ61_37320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960229.1"
                     /translation="MIEVRTYPGASSSTRPGEQHITSGRRLAYASGNAALIEKDTEEV
                     TPLLDVPSGGRRKACTNTVLGLALLAACGEPAPSPVAPGSGGAGGAATAGAAGDDSSN
                     AGESAQAGAQNTGGGGDSGGAQNSGGAQNTGIAGAGTPAESGAGGDAGYSGNSDAQTG
                     GSLGQAGAAGGGGESVEPKPVAPRPSVLMRGYNKYRTNSNLRESTLRPERITEDGFGK
                     LFCKPVDDEIYGQILYFSDLEWTDGHSRDVVFVATMNDSVYAYDAQDAKAPPLWQVNF
                     TSPKEGIVPVPTSDLSGPICHIYQDMSHQVGITSTPAIDPETQTLYVVARTKEPSGYA
                     QRLHALDMQTGQERPGSPVSIDATAPGTAPDASNGKLRFNPRLNNQRSALLLQDGIVY
                     IAFASHCDAGPYHGWLLGYDARTLEQKIVFNTTPDGQNGGIWMSGAGPAVDGSGALYL
                     ITGNGSATLDAPIPNRGQSFIKLTRSGAGLRVADWFTPSEYLTIEQEDRDLGSSGPMF
                     IPDARLIVSGNKQGSLYMLDANALGQFAEGRNPRVVQRVELVEQTHVHGTPGYWHSRA
                     GAYVYVMAEEDHLKQFELENRQLKLLHSSSLSAPVDPFPASFTTMPGGILSISAHGDE
                     PSSGLVWVSVPSRLDANHAVVPGVLRVFAAEDVTHELWNSDQNFARDAFGNFAKFNPP
                     TVVNGRVYVPTFSGQFCVYGQLPNQASPP"
     gene            complement(18923..19792)
                     /locus_tag="VFQ61_37325"
     CDS             complement(18923..19792)
                     /locus_tag="VFQ61_37325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET9960230.1"
                     /translation="MRIATWNVNSLKARQERVAGWLDRASPDVLMLQETKAGQDDVET
                     DEFSRQGYDFAHFGEGRWNGVAIASRVGLRDVVRNFAHLQHSDGPHVGDDEPLAEARM
                     MSALCGDVRVTTIYAPNGRSLGSTFYQAKLVWYERLARWLRETQDPNQPHVMGGDFNI
                     APQDADVWDPVACHGGTHVSPAERAAFEALKAWGFVDAYRVRHPEARRYTWWDYRAGA
                     FQKNLGMRIDHLLVTPSLAERLVWIEIDREARKGKPTPSDHAPLVMDIDAPGRPFDAG
                     WAGAEQRLAARSR"
     gene            complement(19884..20582)
                     /locus_tag="VFQ61_37330"
     CDS             complement(19884..20582)
                     /locus_tag="VFQ61_37330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET9960231.1"
                     /translation="MIELYTAPTPNGHKVSIALEEMELPYQVRLIDFKQREQKAPEYL
                     KINPNGRIPTIVDDGFAVFESGAILLYLAEKTGRFLPQGSRERSRVIQWLMFQMAGVG
                     PMMGQANVFYRYFPEKVQPAIDRYQAEVRRLFGVLDTQLAASEFIAGEYSIADMATWP
                     WVRGHEWAGVPIDEFAHLQRWLGAVGARPAVQRGVQVPEDPLPRLLGKEQSEYVQLAS
                     QMVETGRGESKRAT"
     gene            20783..21703
                     /locus_tag="VFQ61_37335"
     CDS             20783..21703
                     /locus_tag="VFQ61_37335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BtuF-related (seleno)protein"
                     /protein_id="HET9960232.1"
                     /translation="MPRVVSLLPSATEVVYALGAQDALLGVSHECDYPELARSKRVLT
                     RSRLATRLDASSLEIDRAVRTTLHNALSIYEVDEPALAELAPEVIITQDLCDVCAVAL
                     GDVRSAVARLAQRDHIEIVCLAPTLLTQVWDDVSKIADAIGESASGRRLRANLERRVS
                     EIAQRAHSSAVRPRVASIEWLEPLMLGGLWMPELIELAGGVPVGVEAGQPAPTVGTSE
                     LCALKPDVIVLKPCGFSLQRALDERAPLERLRRELGPSTRLFVTDGNAFFNRPGPRLV
                     ESLEILAACLHPKLFADLAERHRAVIVECP"
     gene            complement(21732..23573)
                     /locus_tag="VFQ61_37340"
     CDS             complement(21732..23573)
                     /locus_tag="VFQ61_37340"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET9960233.1"
                     /translation="MRNLSLGPLADFVLVVAILALGQPVLVPVALAFYLAFVLTPPVD
                     ALERIGVPRGLSVTSVVAAALAAVAVFGTVLISQAADLGRQMQRYSAQMSEKLTRVRS
                     DRFGVVEDLTKAINQLGKSLDPQVIRAEDVTPVQVVSGGFSTFERLEHALGPLLRPVG
                     ISVLVIVMAIFVLGHREDLRGRLIQLMGTQNVTVTTRTMAEAVKRVGHLLLTQAYINV
                     GFGVVIAGGLYFIGVPYALLWGVLAGVLRFVPLLGAVIATLLPSLAAFAIFPGWREVL
                     LTIGLFLVVDTLVANFLEPMVLGKRTGVSALALLISALFWTWLWGPLGLVLATPLTVC
                     AAVVGRHVPQLSFLSVALGDEPVLNAEVEFYQRTLAKASKDAYRLVRRRALATSLPDT
                     FDELFLPALGLMVSEQNLQGLAQGTSERLVRDINDIVDRLVADEGLKAALATNAKATA
                     LDDLARTREDAAGDRRGTIVAIPAESESDVLLVRMLTMTLGEDLPRVVPLPRAGRAKL
                     LADAIQHQPSAICIAALPPSGNANARFLCRRLRAELPDVRLIVLLPEARNKRSREAAA
                     RLREAGASAVVYGLREAARLLIDPAATSQRGGRSVSTPELAVASTRS"
     gene            complement(23561..23929)
                     /locus_tag="VFQ61_37345"
     CDS             complement(23561..23929)
                     /locus_tag="VFQ61_37345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9960234.1"
                     /translation="MARNGCKVLVVDDDADSRDALLRRLETEGYFAAEAENGAQAIEL
                     LEGGLDPDLVLTDLMMPVMSGWDLCETLKGTAAWRSIPIVVLCGMSAEQRGKLQVDDA
                     FEKPTDFSMLLNRISELCGI"
     gene            24292..25182
                     /locus_tag="VFQ61_37350"
     CDS             24292..25182
                     /locus_tag="VFQ61_37350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9960235.1"
                     /translation="MFDWDDVRYFLAVARSGSLSAAARVLQVAQPTVGRRITAFERRL
                     GAELFERTLEGQKLTATGRRVLATAERMEEEALALEQQTGRDPTLRGRVRITASEWLI
                     QRLLAPILVPFNRAHPELELELWADIRHLSLTRREADIAVRPSPFEQGSIVQRVVAEL
                     KFGLYASDHYLLERGVPDFSRACEGHVLIAMSESLGKIPDVEFLPRVAAKARIATRTN
                     GREPMAVLAAAGLGIACLPRFLGDATAGLRHLPCPEPEPTRRLYVGFLGDDRAASRIQ
                     SCVTFLAHTIPRLRSALAPS"
     gene            complement(25218..26099)
                     /locus_tag="VFQ61_37355"
     CDS             complement(25218..26099)
                     /locus_tag="VFQ61_37355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9960236.1"
                     /translation="MRIHHLNCVSACPLGGALMDGFSHGSLRGKLVSHCLLVETPNCL
                     VLVDTGYGLRDVENPRSRLNPLFLALMRPELRAEMTAIRQIERLGFGARDVQHIALSH
                     LDFDHAGGLDDFPNATVHLFGDEAGAARAQRTLLDRMRYRPQQWHTQSMWNLLEPGAG
                     ETWFGFSGVRELPGVGPDLLMVPLIGHTLGHAGIAVRREQDWLFYAADAYFYHAEMDP
                     DRPYCTPGLRLYQAMMEKDRSLRLLNQRRLRELHAAQPAVTIFCAHDVVEFERLSGRS
                     IRSPVAAPDRVPAQQPA"
     gene            complement(26096..27817)
                     /locus_tag="VFQ61_37360"
     CDS             complement(26096..27817)
                     /locus_tag="VFQ61_37360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HET9960237.1"
                     /translation="MMKQGAPEGANKIGLSELRREARRRFGVTSFRPGQLDLLRCVLA
                     GRDAVGVLPTGAGKSLCFQLPALFLPKPTLVVSPLIALMQDQQEKTQALQIASAKVDS
                     TLSAPEEREVRDEVKRGSHDLVYVTPERLENAEYLQLLGRAGASLVVIDEAHCVSRWG
                     HDFRPAYLALRTAIEALGGPPVLALTATATPAIVADITEQLGLRSPRVINTGIDRENL
                     FLEVHRTPSEAAKQERVIELVRGEAGPKIVYAATVRHTEQVFEWLTQAGVSAGRYHGK
                     LPTKERQRVQRAFMADEIETMVATNAFGLGVDKPNVRHVIHYEFPDSLEAYYQEAGRA
                     GRDGEPCRASLLYRLEDKRVQSYFLGGKHPTGQQVMGVLRALETLGAEHGRVQLTLDR
                     IRAVADLPKNRVKVILAQLERARLIRRVGLKFEQERVVRGPGEIERLVNEHDARHAED
                     RERIEAIMHYAQTTECRWKVLQRYFGNESASDCGHCDNCRDQPATALARGCELVESDA
                     PKSQLTDSAANTESDATNARPKFDVGAVVRHARFGRGSVLRQDTSRVLVAFQSGDEKW
                     ILSSYLE"
     gene            28017..28475
                     /gene="msrB"
                     /locus_tag="VFQ61_37365"
     CDS             28017..28475
                     /gene="msrB"
                     /locus_tag="VFQ61_37365"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET9960238.1"
                     /translation="MASPLVSSSGFDLTPPSETQRERRTSELSPEQAHVLLRHGTEAP
                     FCGGLLGQKSPGTYCCGFCGLPLFRQETKFESGTGWPSFYAPFDPSHVKEVRDTSYGM
                     LRVETRCARCDSHQGHVFPDGPPPTRLRYCINSVALRFVAEGEPIPNLLK"
BASE COUNT         5311 a         9454 c         8758 g         5321 t
ORIGIN      
        1 ttcgtcgagt tcccctgccc tcctgatttg gtggggctca actactacgt cacgagtgag
       61 cgcttcctgg atagtcgggt ggagctctat ccaccagcga tggtcggcgg caacggccga
      121 cacgcgtatg cggacgtgga agcggtgcgc gtgtgcgccg acggtttggt cggtccaact
      181 tgcttcctgg tggatgctta cgaacgctac gacaagcccg tggcgattac ggaggctcac
      241 cttggctgct caccggagca gcagattcgc tggctggcct acgtgtggaa ctcggcacag
      301 acggcgcacg cgctcggcgt ggacgtgcgg gccgtgaccg catgggcgct cttcggtgcg
      361 tacggctggg accgactcgt taccgaagga cctgggcgct acgaaccggg ggtgttcgac
      421 gtttcgccgg gtttcccaat cgagacgccg ctgagcgact tcgtacgagc actggcgagc
      481 ggcgaatttt cgccgctggc accgggctgg tgggaggtcg aggcgcgact gctgtacgaa
      541 ccgcatgtcg cgcccaagcc cgagcgcaag tctcgagccg cgtaaaacct atgcttcgct
      601 cgtgagaacc aaccatggcc aattcgatac tcgatatctc gcagccacga tcttcgtcac
      661 ccaccgcctc aaacatgagg cggcgcgagc tactgctggg cgtggtcacg ggcggttttg
      721 cgatgaccag cttgggctgc tcgcgcaacg cgagcagcgg ctccaactcc aacgcggcgg
      781 gacctagtca gggcccgcag gggaacaccc cgccggggac gggcaaaacc aacccgcggc
      841 aactcggtgt ggcgctgctc ggcctcggca actattcgga gacccaactg gccccgagtt
      901 tgaagctcac gcggcattgc agcctgcgcg gcatcgtcac gggaagcccc gacaaaatcc
      961 cgcgttggca gaaggaatat ggaattcccg accgcaatgt ttacgactac ggctctctgc
     1021 cgcgcattgc cgacaactca gccatcgaca tcgtgtacat cgtggtgcca actgcgctac
     1081 acgccaagta cgccgtacag gctgccgaag cgggcaagca cgtctggtgc gagaagccga
     1141 tggcgatgac cgtcgaggag tgccagaaga tcatcgatgc gtgccgcgcc aatcacgtct
     1201 cgctggcgat tggctatcgc atgcatcacg agcccaacac ccaaaccgta atgcagttcg
     1261 cgcgcgagaa gccgtacggc gccatcaagg ccgtgcgtgc catcgccggt gatcgttcca
     1321 atggcgagaa cacctggcgt atggtacggg ccatgggtgg aggggcgctc tacgatatgg
     1381 gcgtctacag catcaacgcg atccgttatg cttccggaga ggagcccgtc cgcgtggtgc
     1441 gcgcgcggca gtgggcagat cgtccggagc tgtttcgcga cgtagacgag aacaccgaat
     1501 tcgagctcga actcccgtcg ggcgcgattg cttacggaaa ggcaagtcgt ttcgacaaga
     1561 ccaatcagct gcgggtcgag gcgcagcggg gctggtatca gctggagccc atgcaagcct
     1621 acgaaggcgt gcaaggcgag acgagcgacg gcaagaagct ggacaagaag gtggataaac
     1681 agcaggcccg tcaaatggac gacgaagccc tggcgttact cgaaggccgg cccgctatgg
     1741 tccccggaga agagggacag cgcgacatac gcctcgtcca agccatccta caggccgcga
     1801 gcacggacaa gcccgtcgcg ctgtgattgt gcctgtctga acgcgaagca ggctagtgtt
     1861 ctgcgccggg gacgaacaga atacttgccg ccaccaccgc gcgggcgcag ggatctttga
     1921 gctcacttag aagtagagtg cgcagccgag gcaaccaagc cgcttcggcg attcccgcct
     1981 gggccgcttg gcgacgcagt cgctgcaaca tggctcgccc gtttggcccg acttcgctag
     2041 tcaacaacga ctcgatttgc tcgagccggt aggcgctcgc ccgatcgtcg atcggaggct
     2101 cgaccgaagc cgaaatcacc ggcgctgcgg ctgctgtcgg cgcaaagctg gcactgggtt
     2161 tctcgacgat gaccggtgcc gagatcatcg agggaacggt agaggcatcc tgggaacccg
     2221 cgctgacgtc ttgcccgagc agcacaccga gctcttccat ggcaatggca acgctcagct
     2281 ccagctctcc ccgcgcgacc ttgggagagg ccacaatggc tagcactccg gcattcggca
     2341 agggccaact gaacagggtg gcccgcggcc aatggaatac acacgcggag cgatcgtagc
     2401 cagccttgtc ggagagttgg ccgagtcggg acaggccgga gccaatttgg ttcagacgcg
     2461 ccgagtcgta ggccttgggt agactcgacg caaggcatct gccgccttgc cagacgaaag
     2521 cacccacggt gccttcgagg cccttcatct cgtcgcacac actctggatg ttgctcattt
     2581 ctcgagtcgc tttcgcaaga cgtcgatatc catgtcgacg gacgttttca ctgcgcgcat
     2641 gcccgtggag ttgaccaggc agaccaggcc ttgaccgcca gcagcgtgca cctcgaagcc
     2701 ttgcaagtct ttgacggcga gcgcgctccc gatcgcggcg gcaaccttca acgtcagcgc
     2761 tgcgagcccc gcgtcgtact gcgtgaacgt accttcccgc gcgatcacac gcccagccgc
     2821 gtccaacgtt gctcggccga gctcgcccac cggaacctct tccccggccg cagccgcagc
     2881 cgcaggctcg gcggtggctt cgttacgggc agcgtcgctg ggcgagctct cggctggagc
     2941 cgccaccttg tgaacgccac tattcaaccg accttcgggt ctcgatttcg cgcgttctct
     3001 gacctgccgc tcgtagaact ctttgactgc cgccagcatg cgctcgtgca gatcagccgt
     3061 gcgccgctct ggttcggcta cgagccccca cgcaaactcg aactcgccct cggtcatgcc
     3121 cagcaccttc tcgaggcact cggggccatg ctcggagccg gaggtcaccg ccatcaagcg
     3181 accatgctcg tagtacagct cgctatcgga ctgttcgttg aacacgagca gtcgaccact
     3241 gctgcccttc gagaccgaaa cgcgcaagag gtcatgaatg ccaatggcct cgatccgccc
     3301 gcgcagcgtg gtgctgccag acgcgggctc cgcggaccga tccggtgttc tgtcgttgat
     3361 tgctgcggtt tgggtctgag tagccatgtc gtaccgttgc tatgagtttt gctcacgccc
     3421 ttgaacgcga aaatgacacg gaactggaag cgatggccat gagccctcgg tcgtgcgcct
     3481 ccgtcctgcc cgggcacgac cgccgggatt cgcgagcctt aggatagctc accattcggg
     3541 ctccgcgaat ccatatgaac ggccgcggga gcagctccgt aatcgtccca cccagagttg
     3601 tctctagaag cgacggggcc agcttcgatt gtctcacgct tccaaaacgg gccaagattc
     3661 ttgctcccag ttacgtggtg cggttttccc agacaccggg gctcgcctgc cgtgcatgtc
     3721 gctcactccg gttccgagga tcccctcggc gtgctcaagg cgcgacgcgt ttatacccga
     3781 ttctcacaca tccgaaatgt ttgccacgaa tgcgtaacgg caccgagagg tcgaataggt
     3841 tctcgctcgt gtcccgggag tacggttgaa tcaggaaagg ggtctcgttg cgcgaagcgt
     3901 tgaacccgac tgtgtcaccg aagatgcgct tactgcggtt gttgaccagg tccacggcgc
     3961 gattgcccgt gagcggttgc gagaactgtc gattgtgggt cggcacataa ccattcagat
     4021 cggtaacgac cgtgtactga atcgcggcgt tgcgcgtcgc gtgcttgtcc agcagcggct
     4081 ggagatcgcg atccgccagc gcgtcatagt ccgtgttgta tttgaccggg ttagtgttcc
     4141 ggattgggta gtagaggcgc gacaatagac gctcttcttt gagcgcgtcc gaggccaacc
     4201 aacgttccat gaccgcccca gcctcactgg ctaggtcgtt cgcgatccgg agaatttctt
     4261 gatcctgcgc cgaccggtcc tcggcgcgcg gggccgcggg ctcttccacg cgagcggctg
     4321 cattcttctc gcgcgcgcct tcgcgagctc cttccgcgct gccgctcgct gcgatcagcg
     4381 tcgttccgca tacggctgcg aacggtaacc acgttttcca tctgttggac atctgggtgc
     4441 tcccttcgag ttccgccagt cactcgccga gtcgggccat cgcggccttc aagcttgcac
     4501 gtagccgatc gagcgacttt gccatcgtcc cgacttcgtc ggtagtagcg ggcttgattg
     4561 gtgtttccag cccctcgccg atgctcaatt gatccgcaat ctgcgtgagc gcggtgagcg
     4621 gttgcatgga ccgacggatc atcaagaaga tgacccctgc gctcgccacc gcaaagacgg
     4681 cgaacatacc cgtgagagtc ccgatcaaac taccacgctt gcgcgcgatc tcatccatcg
     4741 agaccagcac ccggaaggct ccccagtgct tgccttttac gaacacgggc gaggaaacgt
     4801 accaaagcat ggccccagtg tcccgccgat aaggctggat cagcacggga tccacgctct
     4861 tggccgcgcg catcgcgata tcgtcattga agatgcgctt ggtgcggttg ccttccaaat
     4921 ccttgaccgg gtcaccggtg agaggcttca tgaacttgct gttgtgcgtc ggcacgtagc
     4981 cgttcacgtc gcccgcaatc gccgcgacga agtcgtcgct ctctagaaat cgatccaaga
     5041 agcccaccgc gatgcgatcc gtgatgaagt cgtagcgcgt atggtacttg ggcttgccgc
     5101 cccaatcgta gcccttgatt tcctcgtagt tcgtgtcgaa gacgtcctgc acggtgacat
     5161 gcccgtcgtc gacggcttgc tccagcagct ggccgtaacc ttgtgcgccg agctgcgccg
     5221 agagtttggc cttgctcagc gtcatgtcct ccatggccgc ggtttgatgc gagatgatca
     5281 cgcccgcgga aatggatgtc agcgcgagcg tgaccgccgc caaggtcaac gatactttca
     5341 aactgatgct attacgaatg gctgcgaaga ccatcgtctc ctcctcccgc cccggcttct
     5401 agcttggttg ctagataact ctcagggctg tttttctgaa ctgcgccgca tcgctcggat
     5461 cttcgatgct cttcgaaagc tccgcgacca gcgctgaggt ttggtctttc gagaagggtc
     5521 ggtcgccgca aactttgcgc acggcttctt tgacgaagac ctttgccatt ggccccacgt
     5581 tgcgagccaa tgctttggtg catgcggtga gggccgccga actcgctgga tccagcaccg
     5641 tcacgccaga ggatgctagt gctgagctgc caaatcccga ccagctgagc ccgctcgtcg
     5701 cgacctccgc gacttgcgtc ttgaagggag ccgctggatt ggcttggcta ggagcgtggc
     5761 tgactggggt cgctggcgcg gaggatgtcc cgacggcgcc gagggtaccg cgcgcattcg
     5821 cgagattcgc cactccgccg gcggcttccg ccagtgccct gagcttggcc acggccacgc
     5881 gaatcgctac tccggcgaac gaggggttga gtcgggtatc ggccaccagg accaaacaga
     5941 gtgccggact cgaagcgggc ttggtcccag tcacgttgcg gaccagcagt ttgccttcgg
     6001 cgaactgcgt ggtcaccgat tcccaatctt catgtagcag cttgaccgag tcgagcgccg
     6061 acgcaatcgc gcggctcgcc tgggagagca ggtcaccatc gtagatggag tgtgcctggt
     6121 aagcgatcac tcgaccgttg gagtcggcca gaatcgtacc gtggaccccc tccagctcgt
     6181 tgagggtctt gagaatgttc tccattgaac taccctcacc gcttgggcgt atcttgtagt
     6241 ttcttctcta tggccgccag cggcttgtcg gggtcgacat acacgccgag gatttcttgg
     6301 tcgtggatgg tgatgaccac cgatttcgtg gggcccacga tagccgtcct ctgaatgcca
     6361 ccgagaccga gaagttcgcc agcttgaccg agcgacttca tcgaataggc gtgaacggcg
     6421 cccgccacct cgccgtcgat gttccccgca acatcgagta gagcgcccga cgggtcactc
     6481 aaaactgctc cttgaacctc ggggaacttg gcgatgacgt tgaagtcctt catgaccttg
     6541 caccttctcc gctttcctgt gcaccgcgac ctccgcacgt cagcgcactc ccgtcgcgct
     6601 accgcggtgg ccgggctagc cgacgagcga cgctgctcct cacagccatc gctgccacgc
     6661 aattcaatac gggtgcgtcc gagcgcggct tagcctaacc aagaggaata aatgacaagg
     6721 gtcgcctcct gtttcgtggg cggtgccgat ttttcccgcg cttcgaacac ttcacgcgga
     6781 gatcctcgat gcggcgtgtc gctgccgact cgaagcagca agaaaagcga cgtcgagtga
     6841 cgtgcggcct actctggatc atgaggattg gcgccgtctg cgctgaatgg ctcgcttcag
     6901 ggggcaatcc cggaccgatc ctgccgtact cgtgggtggt ggcgcccggc acagcccaag
     6961 cagtccaaac gagccgggcc cggagctggg ctaactagca gcccaaccgt gagcgccgct
     7021 gcagcccatc gcgagcggcg tgaatcccaa ggcagtcatg gagaagcctc gatgagtgcg
     7081 tcgcaacttg tgtcgaaaat cccgggggtg cggcgctgta tcgtgaccga tggcgtcggg
     7141 cacgtgctcg aagggccggc tgaggagcgc agcacggccg aggtctcgcg cgccgccgta
     7201 gggctgttcc atcgcttgtg tgaaggggga gcgctgctcg ggctcggtca gccccagctc
     7261 gtcacggtgc gggcggcaga aggctctcgc gtcgtcggtt ttcgtggcga ggcgtcgatt
     7321 tcggtcgaag tagacccgaa gctcccgcgg agcatcggcc aggtcgagtc cacactgctc
     7381 cggaatgact ggaccgctga gggagactgg ctgatcgacg agtcggacat cgagtgccta
     7441 gctcccgagg gcgagaccgc cgctcctccc tggtcgccct cgaacgccga cgtgggttgg
     7501 acggagttgc gcagcgcact cgcccggagc gacgccggtc gggttcggct gcaacggcct
     7561 agaaggagcg cggcacggcc gtcggggttg cagtcagcag gacttggtat cccgggcttc
     7621 gagtccctga tcgatgcgct caccaaggcc gtagagtcaa tcgacgccgg agacaatcac
     7681 gccggtctcg aagcattagc gctactgacg gtcgagctcc gagatactcc gcccgcgtcg
     7741 acctcgctgc gttggttgac cgagctctgg agcgcgcgag cgattctgca cgccggagag
     7801 gctttggacg cagccgatgc gcacgcgcag gcggcgctag cggaggcggg tgagctcgat
     7861 ctgcgagcgc gcgctctcag caccctgttg atcgccgagg tggcgttgta tcggcgccat
     7921 ctcaaccgtt gcttggagct ggtgcgcgac gcacgtgaac tgttcacgga tctgaaggac
     7981 gaccacgagc tcgcgagctg ctggctgctc gaagcgcgtg cgctcgccgc gtccgtgcgc
     8041 gacaacgaga gcctgcgcgc ggccgaacag gcgcaccatc atcgcccgtc ttggcctccg
     8101 ccattgacgt tcttggtgaa acgcgccttg tgcgccggtc gcgtgtcgga cgcggagcaa
     8161 gccctcgagc cgatgctcgc tcgtgaaacg ccgccactcg aggtgaccgc ccaccgctgt
     8221 ctgctcgatc acgtaaaggc cggaacgatc gcctcacaca ccgcctacga atacttgcgc
     8281 ctgctcgact cgcccccgac ccgcggcacg ctccgacagc tcgaggcttt ggtgcgcgcg
     8341 ttcccgacgg tcgagcagtt ccaggagacg ctgggttgca agctgctcca cgcgggtcac
     8401 tacgagagcg ccgcattggc gctcgaacgg ctcgcggtga agcgtggtgc accgagtgag
     8461 cagctgcagg ccgcacttac cctggctcgc gcatgcctcg gcagaactca ccgatcgagc
     8521 caggcggacc atgactgacg ccgcccgtct gaccggcgcc aagctcggca attatcggct
     8581 cgaacgcatg ctcgggcgag gccgcatggg agtggtgtat ctcgcgcggg acgaagcgct
     8641 gctgcgaccg accgccgtca agattctctc ctgggaaatc gaggaccacg aagggcaaga
     8701 cccgcaagct tggttcctcg cggaagcacg caacgtggcg cgcatcaatc acccgcacgt
     8761 cgtgcagatt tacggggtcg cgaagcaccc cccgcattgc tacatcgcca tggagtacgt
     8821 gcccggcatg ggcgcggaca gctggatgca agaccatggc cctttccccg cacgccgcgc
     8881 gaccgagatc ctactccaaa cggcgctcgc gttgcaggcc gcccacgacg caggcgtcgt
     8941 gcaccgcgac atcaagcctg gcaatctcct aatcacggga gacggcgcgg tgaaactcgg
     9001 ggacttcgga atggctttgc acgtggcgag cgcccatgcg ggtggccgcg ttcgcgccgg
     9061 tacgcccttc tacaccgccc cagagatttg gtgcggacag accgccgccc ccgcgagtga
     9121 tctatacgca ctgggagcca cgtactatca cttgctcact ggaaatccgc cgtttcaggc
     9181 tgcgaacctg gagacgttga tcgcagcgca tctgaacgct ccgctgccgg atcatccttt
     9241 actagcggac acgccagagg cttgctggga gctgctccgt cgctgtctgg ctaaatcacc
     9301 gaaggagcgc ttcgagtcag cgcaggcgtt gagttgggag gcgcgcgggc tgctcaaagc
     9361 cctcaacggc tccgcgtcat catcgcagcc tctgagccac acgatgccag cggctgcctg
     9421 cagcggcccg gctcgaacgc cggatgccca cgaagtaggg gcaaccccaa aagccagccg
     9481 gattcaacac gccggcgacg cccgcaacat cctcgacgct ttgaaaatcg acccaacccc
     9541 gaaagtcgac ccggctccga agatcaacgc tccgctcgtc gccaaagctg cggtcgagca
     9601 gggttggtgg gtcgggcttg gtttcgatcg aaagccattt tcagaactcg agttgggtga
     9661 agcaccgtac cccggagaac cctttgcggg actgtgggtc gagctattgc atgccttcga
     9721 cgatgctccc ggtgagcagg actcagtgcc cgctcggtcg ccgagaggac ccctggcggg
     9781 gaacacggcg ggcaccgtgt tactcagcgg caggtcgggt agcggacgca cgacactcgt
     9841 gcgccgcctt gcgacagaac acgagccgtt cgcaccggtg gcgtacgtcg atttggcacg
     9901 agagctcgag ccgaatctga ccgcctcacg aaaaacctgg gccttgatac gccgcagctt
     9961 cggcttgctc agcgtgccca gccccaacag ccgcgagttg gactctctac tcgatcaact
    10021 cggccatttg cggcccgatc agagcgcgcc cgctctcctg attctagatg gcttcgagcc
    10081 ccgccatttt tcccgtgcag atcttgctct gcttctgcgg gccgctcgcg ccacacgcgc
    10141 gctccgcatc gtgctcgtag ggggcccaga cctggtcgcc caaatgcgcg agtgcgacct
    10201 ttgggagccg cgctgcttgg cgggtatctt gaccgtgccg ccgctcagcg ccggtcaaac
    10261 ccttcgatac acgacggatt ggctacgtgc gactcgagct ccggaccgca acgtgattct
    10321 cttcacgccc gacgcgagcc tgattctggc gcaccgcggg gagggcaatc tggcgctcat
    10381 caatcgaggc gcgaccagca tgctccgcat cgcacaggaa gagaatcgca gggtcctttc
    10441 ctcttggcat gcctgggtcg ccgcgggcgg ctcgggggag gctctctctg cgagcccgtc
    10501 cccaccccga ccctggcctt cggcggaagt gctggagatc atcaatcgag ggcgtcgaga
    10561 cgccggcttg ccggtgcgtt cgcgctagcg agcatcagaa tccagtgagc ggaccgcagc
    10621 cgggctgatc tccataaccc gctacttgta cgcccatcag ctgcagcacg gtcgtgcaca
    10681 attgatgatg agattgggcg ccttcgtaga atacgccgga tcgagtcagg cgccctcccg
    10741 cgcgccccaa catcacgacc ggtacgttat ccaaccggtg gaagcccgtc gcgttctcgt
    10801 ttgcccagac taccagcgag tgatccagga ccgagccatc ctcctcggga atggcttgca
    10861 gctgctgagc gagcttggcc acgtgcatcg ccacccaccg gctgatctga gtcatgatct
    10921 ccgcgatcgc aggattgctg ccatcgtccg cgtccaggtg cgccacgtcg gagtgattgt
    10981 cgatgttgat cccgagccaa cgatagaacc actgattgcc cgacatcccg attgggaaag
    11041 tcacgatccg cgtcaggttg caggccagcg ccaccgcgac cgcgtcgagc atcaattcga
    11101 gcaggcgagg tacgttgtct tccaagctcg ggtcgagccc ggcctcggga gccttgggtt
    11161 tggcgcaatt gctttgccct tccaagctgg ccgtcagtcg agcttcgtat tcgcgcaggg
    11221 cggtggtgtg cgcgtcgagc ttctgccgct gattcgggcc aagccgcgcc gccagcgccg
    11281 cgagatcccg cgcgctggcg tccaaaaccg atcgtcgaag ctggagctgt tggcgggctt
    11341 gcgcgggctc cagctccgaa gttcgaaaca aggatgcata aactcgatag ggatccacct
    11401 gagtgggaat ctgctgccgc ggcccgatat agctgaaaga gcgccgatgg ccgttcttcg
    11461 gcgccacgtc ggccgcgagc acccccacgt tcaaggttgg gaacagaacc ttgggcgcca
    11521 gcgccgcctt caggatttga tcgatggaag gaccgccccc gaaggcgtcg tcgggtttac
    11581 cgatggtcgg aactccggtc cataaactgc tgaagccgcg atcgtgctcg ttgccgggtc
    11641 ctgtcgtcag attgttcacg ccgcgaatga gcagggttcg ctccgccaag ctcggctcac
    11701 cgaggatggg ctcgagaatg ggcgagctgc cggtgttcgg atcgggccag aaggtcgcct
    11761 gatgcgtgcc gttcgcctgc atgaatatca gcaagcgtgg gccgctcttc tcgggctccg
    11821 cgaacgcttc ccgcagaaag ctcggaacga gcaaggtcag ccctgccgcc cggagtaagt
    11881 ctcgtcgacc aaagctagct tgcttcatgg ctctcgcctt ctccgcatga agttacgctg
    11941 gagcgccgtc tccactgcta gatcggcaaa cgaccgcgat cctcgagcga agaacttctt
    12001 gagcggctcg agcacgcaag cgtccgcgct ctcctcctcg cgcccctcac tgaaccggaa
    12061 ccactgaatc gctacgcatt cgcgagcctg ctggctcttc aaaaggagca agctaagctc
    12121 ggccggcccc gaaaagggcc catccacgtc ggtgcccacc agcgcgccgc tcgaatcgac
    12181 gggcaggtta ttttcctgct cccgatagcg gcccaaggca tctatcatct ctagtccaaa
    12241 gcccaaagga tcgatcaaac ggtgacatgc ttggcaggcc tggtctcgag tatgcacctc
    12301 gaacttctgg cgtgtagttc cggtctcgtc gcccgggggc ggagaagtga acatcagccc
    12361 caccggcggc gggatgggcg cgcacagcaa gcggctccgg acgttgaccg cgcggtcgac
    12421 ggggctagac cgatggatgg gcgaatgcgc cgcctcccat ccggcgagcc caagcagccc
    12481 acggcgaatc cgcgtgttca agggcacggc gacgaactcc ccagtgctcg gcagacgcgg
    12541 cgcctctgcc ggcgcgtaat cggcagcgta gataacccga aatagattct gattcatgaa
    12601 cgtggtatca gcgccgagca ccgcctccca cgtcccgccg ccgggtccca aagcacgctc
    12661 caggaacagc tcgatttcac gatcgagatc cgctttcacc gccggagtgt agatggggaa
    12721 acgcaccggt tctttattga ccaggcgcaa atccaaaaca ccaaacaggc cgcgaacgaa
    12781 gtgagctagg tgctgccgcc ccgcatcgct cgtcaccaat ctgcgcgctt gcttttcgac
    12841 ctcttcacgt tgcgaaagcc gctccggcgc ctgcacccag gccacgagct ccgcgtccgg
    12901 cctggccccg gtgagcgcga acgagagcgc gttcaccttc tcttcgggcg tcagctccgc
    12961 ggtcgcggtg cttccctcca cgccgagctc cgtgcgatac aagaaaaacg gagacaaaag
    13021 caccgactcg gcgatcaggc gaatgcccgt cgcgtaaccg ccagctcgat tgccagtatc
    13081 atagagcgcc agcagactct cgagttctgt ctcgctgata tcacgaccat aggccaagct
    13141 cgcaaaaggc tgggcaaagg cgcgcgcgca atcacgtgga gcgtccccct cgtcgccgca
    13201 cggcgctaag cgatcgacgt ccaagttggc cgccgaaagt tcagccgcga tcgcgtactc
    13261 gtcgaaattg ccggcggtta tattgagggc atcggcgttg ttgtcgaagc cgtgcacgat
    13321 tgggtccggc agactaggtg cgacgggctc tgcgccggtg agcgcaatta cgctctgtac
    13381 atattctctt gccgtgagtc ggcgtacgcg ccgttcggtc accggctcac agccggtaga
    13441 agaggaccct ccactcgcgg atgacgtgct atcgggtgcg ccgccggaac tcgaagtgtt
    13501 ggcgcccccg tcgctcgagc gtacggcgct ggagccgccg gcagctggag acgggtcggt
    13561 gattgccgct ccgggctcgc cctcgggccc ggagcagccg gtagccacca tgaccaaccc
    13621 gatcactaga ccaccgatgc caccactcga gaacacggcc tcaggctaac acaaggttgg
    13681 agtagggctg gcccggaagt cagaaccggc accgcaattt gcgcaacaca ccgaaaaatt
    13741 gcgcccgtca cgcacaccac gactctcgga atgccggctc gcgccgtgca aatccggagt
    13801 tggccgcggg ccattgagtg gatgttgttg gggggggcct gcgcggtgag gagggggata
    13861 ggagggatgg ggctgcgctc gcgaatgatc gtgacgggcg gtcgcgctcg tagccctgcg
    13921 gccgccgccg agtcagagca ggctcgcccc cgattcgagg accgggttca tcgcatctgt
    13981 cgatgacgct gagccggctg atcgtggtcg gtctgggcct gagcttcgtc aagatgcgaa
    14041 atcgtgccag attctcggca cgaaattacg cagtttgacc agcgtcaagc cggcttttgg
    14101 gcagccgtga ttgtgggttc actgcggttg ctatcgggta tgttcacttc aaagcttcga
    14161 gtgcgcgctc gtcacttgct tgctcgcaac ttggtagaac taccatgctc aacaagagtc
    14221 gatatggtgt ccgccggccg gatcgtagtc aatttcggag ccgttggtcc gcgccgctcg
    14281 gtctagcgct gctgagtctc gcgtgctcct cgggcgcgaa tggttcgggt agaccccaca
    14341 gcacccgccc cgggcagggc gcggctggca gcgcgtcaga gggccccggc ggctggacca
    14401 gcctggggca aggcggcacc aatcccttgc ttccggtgtc gacggcggga gtcggggcgc
    14461 tctcgaacgg cggagtctcc ggcacggcag gcacgaccag cgaggtgcca tcgttctatt
    14521 gcatcccgga gcccttcccg aaggattaca gctgtcaggg ttcagaaggg ccgtgggcag
    14581 aggcactgca gaaggcgctc tggttcttca acgtgcagaa gtcagggccg ggagtgtatt
    14641 gtaccgacgt gcaatggcgc ggagacgcgc acgtcgggga cggccatatc aagctggatc
    14701 ccagcgatcc gaacggggtg aacctgccgc aggcgttcat tacgcagcac cgcacagagc
    14761 tcgatccgga tgggaacggt gaggtggatt tggcaggcgg ctaccacgac gccggcgatt
    14821 acatcaaatt cacgatgacc acgacctatg cggcttcgat gatcgcctgg agtatgcacg
    14881 aatatccaga cgcctatcgc aagacgagcc tggccgggga agccttaggg cagatccgct
    14941 gggccgcgga ctatctgatg aaggcgacgt tccgggatag cgcgggcaag ctcgtcgcct
    15001 acgcgcacca agtggcggat gccagtgatc actcatgctt ctggatgccc cccgaggtga
    15061 ggcgcccgga gttgtgtccg cgcaaggcgt acttcgtctg ggatgggcaa cccgccgccg
    15121 atgtcacggc ttcggccgcc gccgcgctgg cgctcaccgc gatcgtcacg cgcgagaaca
    15181 gtacctcgcc ggcggatctg gcgtacgccg aacgctgcct ggactacgcc aagtcgctct
    15241 atgagttcgc cgcccggtat cccatgactc gtgagagcga tgacggcggc ctctacgtct
    15301 ccaacagctc gaccgatgac gtggcctggg ccgcaatttg gctgtatttg tcggatccgg
    15361 agcacaatcg agcctatttg gacgcgacca taagcggcaa acaaccctgg ctctccttcg
    15421 gctcccaaag caatctgctc attccggatc cggagctggg ttatcggccc gcgtgggcgg
    15481 aaagctcccc tcactcgtgg gacgcggtgc gcgcaggcgt gatcatgaag ctggctcaaa
    15541 tcacgactga gctcggcgac aagaccgcca ccgattggca gaacatcgct cgacagatgg
    15601 cccaaggctt cgcgaccgga gggtcgacgc ccgatggctt ctacatgtat atgagctggg
    15661 gatccgcgcg ctacaactcc gccgcgcagt tcacgagctt gctgtactcg aagtacttcc
    15721 ccaacgaccc caagtccgct gaattcagca gctgggcgca gcggcaggtc gaccatttgc
    15781 tcggcgcgaa caacctcggg ctgtcataca tcatgggcta tacggacagc tatccgctcc
    15841 agccccatca cgccgcgggc cacgcatcca tctatggttt gccggccaag cccgccgaga
    15901 atcggcacat catttggggc gcgctcgtga atggccctaa cggcaagggc gagcacgtgg
    15961 acgcgcggga cgactacggt tcgaacgagg tcaccatcga ttacaatggt gcgttggtag
    16021 cagcactcgc gggccacctg gccacgcgcg gggcgaacga gtgcccgctc aaggtgttcc
    16081 ctcccctcga accacgaatc gacgagttct acgcgatggg caatctaaac atcgccccag
    16141 gcggctgtcg cacccaggtg aacgtgcgga cgatgaacga gagtattcat cccccgcgct
    16201 acgacgagac gctctccacg cgcttctggt tcgacgccag cgaggtgcgc gccgcaggtt
    16261 tggatcctag tcaagccgtc accctcaacg tcttccgaga cactgggcaa aacgctaccc
    16321 cgcccgaacc cacgggcgtg aagggcccca tccagtgcaa ggagcacacg gacacgtact
    16381 atttcgagtt cgactacacc ggcgcgaagt tctggggcct aatccctgcg atcggcgcgc
    16441 cgcgtgattt gttgctcgac tacgggctcg cctacggcga gaaaaagtgc gagtgggacc
    16501 ccagcaatga ctggagcatg caagatctga aggtggggca gaccgaagag gtcgccaaga
    16561 ctccctacat caccgtgtac agcaagggac ggttgatttg gggcaaagaa ccgccctgtg
    16621 acggcattcc cgacgtgcca gtcgtgcctc cccctccacc tccgctcgag taccccccgc
    16681 gctaaattct ctttacggcg gggacgcttg gttagggagt tgcccgtaga cgcagaactg
    16741 gcccgaaaac gtggggacgt atactcggcc gttcaccacg gtgggcgggt tgaatttggc
    16801 gaagttgccg aaggcatcgc gcgcgaagtt ttggtcacta ttccaaagct cgtgggttac
    16861 gtcctcggcc gcgaacacgc gcaatacgcc gggaaccact gcatgattcg cgtccagccg
    16921 ggaaggaacg ctgacccaaa ccaagcctga actcggctca tccccgtggg cgctgatcga
    16981 gagaatcccc ccgggcatgg tcgtgaagga cgcggggaat ggatccactg gagcgctgag
    17041 gctgctcgaa tggagcaact tcagctgacg attctcgagc tcgaactgct tcaaatggtc
    17101 ttcttcggcc atgacgtaga cgtaggcccc cgcacgagag tgccagtacc ctggcgttcc
    17161 gtggacgtgc gtctgctcga ccaattcgac gcgctgcacg acacgcgggt tccttccttc
    17221 ggcgaactgg cccaaagcat tggcatcgag catgtacaag ctgccttgct tgttgccact
    17281 cacgatcaaa cgcgcgtccg ggatgaacat cggcccgctc gagcccaagt cccgatcctc
    17341 ctgttcgatc gtgaggtatt cactgggtgt aaaccagtcc gcgacgcgta ggcccgcacc
    17401 gctccgcgtc agcttgatga acgattgtcc tcggttggga atgggagcgt ccaaagtcgc
    17461 gcttccgttg ccggtaatca ggtacagcgc acccgatccg tccaccgcgg ggcccgcccc
    17521 ggacatccaa atgccaccat tttgaccgtc cggcgtggtg ttgaatacaa tcttctgctc
    17581 gagggttcga gcgtcgtagc caagcaacca accgtgatag ggccccgcat cgcaatgcga
    17641 ggcaaacgcg atgtatacga taccgtcttg gagcagcaac gccgaacgtt gattgttgag
    17701 gcgcggattg aagcgcagtt tgccgttgct ggcatccggc gcggtgcctg gtgcagtggc
    17761 gtcgatcgag accgggctcc ccggccgctc ttggcccgtt tgcatgtcca aggcgtggag
    17821 ccgttgcgca tagccggacg gctctttggt gcgcgccacc acgtataggg tttgcgtctc
    17881 gggatcgatg gcgggcgtgg aggtaatccc aacttgatgg gacatatcct ggtagatgtg
    17941 acaaatcggc ccgctcagat cggaggtggg cacgggcacg atgccctctt tgggagaggt
    18001 gaagtttact tgccagagcg gcggcgcctt cgcatcctgg gcgtcgtacg cgtacacgct
    18061 atcgttcatg gtcgcgacga acaccacgtc tctactgtgt ccgtcggtcc actccagatc
    18121 actgaaatat agaatctgtc cgtaaatctc gtcatccacg ggcttgcaga acaacttacc
    18181 gaatccatct tcggtaatgc gctccggcct aagggtggac tcccgcaaat tgctattggt
    18241 ccggtatttg ttgtagccgc gcatcaagac ggaagggcga ggcgcaacgg gtttgggctc
    18301 gaccgattcc ccgccacctc ccgcggcgcc agcctgccca agggagccgc cggtctgagc
    18361 gtcggagttc ccggaatagc ctgcgtctcc gcccgcgccc gactctgccg gcgtgcctgc
    18421 gcccgcaatc ccggtgttct gggctccccc gctgttttgc gctccgccgc tgtctccgcc
    18481 tccgccggtg ttttgcgctc ctgcctgagc gctctcgccc gcgttggacg agtcgtcgcc
    18541 cgcagctccg gccgtcgctg ctcccccggc gccgccagat cctggtgcga ctggcgaagg
    18601 agcaggctct ccgcatgcgg cgagcaacgc aagacccaga acagtgttcg tgcacgcctt
    18661 acggcgtcct ccactcggca cgtccagcaa cggcgtgacc tcctcggtat ccttttcgat
    18721 aagcgcggcg ttaccgctcg catatgcgag ccgcctgcct gaggttatgt gctgttcgcc
    18781 ggggcgtgtc gagctagacg cacccgggta agttcgaact tcgatcaccg ggtagtacga
    18841 tctcgaggta ccaaactgct acatcctgct tactgcagcg ggctcactac gctcgattgg
    18901 ggctgatcac cctccgcgtt tcctagcggc tgcgcgccgc caagcgctgt tcggcaccgg
    18961 cccagcccgc atcgaatggc cgtcccggcg cgtcgatgtc catgaccagg ggggcatggt
    19021 cggagggagt tggctttcct ttgcgcgcct ctcggtcgat ctcgatccaa accaggcgtt
    19081 ccgcgaggct cggggtcacg aggaggtgat cgatccgcat cccgagattc ttctggaagg
    19141 cacccgctcg atagtcccac caggtgtaac gtcgcgcttc ggggtgacga acgcgatagg
    19201 catcgacgaa gccccaggcc ttgagcgctt cgaacgccgc gcgctctgcc ggcgaaacgt
    19261 gggtaccgcc gtggcaggcc accggatccc agacgtccgc gtcttggggc gcgatattga
    19321 agtcgcctcc catcacgtgg ggctgattgg ggtcctgcgt ctcgcgcaac caacgcgcga
    19381 ggcgctcgta ccaaacgagc ttggcttgat agaacgtgga gccgagagac cgcccgttag
    19441 gtgcgtagat cgtcgtgact ctcacgtccc cgcagagcgc ggacatcatc cgcgcctccg
    19501 ccaaaggctc atcgtctccg acgtgcggac cgtccgagtg ctgcagatga gcaaagttac
    19561 gcaccacgtc ccgcagtccg acgcggctcg cgatggctac accattccag cgcccttcgc
    19621 cgaagtgcgc gaagtcgtag ccttgccggc tgaactcgtc cgtctcgacg tcgtcctgac
    19681 cagccttcgt ctcttgaagc atcaacacat cgggtgacgc tcgatcgagc caaccggcaa
    19741 cccgctcttg acgcgctttg agagaattca cattccaggt ggcgatccgc atgcgacggc
    19801 tcgcgttcta gcaaatgcct gagcgccgtc gaacgggcgt ttgctttctg gggcgagccc
    19861 aagcggctcg ccccacccca gcactaggtt gctcgcttcg actccccgcg gccggtctcc
    19921 accatttgtg acgcgagctg cacatactcg ctttgctcct ttccgagcag gcgcggcagc
    19981 ggatcctcgg gaacctgcac gccgcgttgc actgcgggcc gggcgcctac tgctccaagc
    20041 cagcgctgca aatgagcaaa ctcgtcgatc gggacccccg cccactcgtg accgcggacc
    20101 cacggccagg ttgccatatc cgcaatcgag tactcgccgg caatgaattc actagcggcc
    20161 agttgcgtgt cgagcacccc gaacaagcgc cgcacctccg cttgatagcg gtcgatggcg
    20221 ggttggacct tctccgggaa gtacctgtag aacacgttcg cctgccccat catggggcca
    20281 acgcccgcca tctgaaacat gagccactgg atcacgcggg agcgctctcg tgatccctgc
    20341 ggcagaaatc gcccggtctt ttcggctaga tagagcagga tcgcgcccga ctcgaagacc
    20401 gcgaaaccat cgtctacgat cgtcgggatg cgcccattcg ggtttatttt cagatactcc
    20461 ggcgccttct gctcacgttg cttgaagtcg atgagccgca cctgatacgg cagctccatc
    20521 tcctcgagtg caatcgatac cttgtgacca ttcggcgttg gcgccgtata gagttcgatc
    20581 atcacgcgct cccttcctga ttcctggtat accccgtgca cagccgcgcc gctcctaccc
    20641 gtttcggcag aatcgctcgt gcggacccga gatcgcgagg ggtaatgctc tcagcgatcg
    20701 gaaacacgac gacacctcgg gccgcgctca tgcgtcggag catgcacgtg ctgttcaggc
    20761 cgtgtatggt gcaagaccct ccatgccccg agttgtatct ttgctaccat ccgcgaccga
    20821 ggtcgtttac gcgctgggcg cccaagatgc cctgctcggc gtttctcacg aatgtgatta
    20881 cccggagttg gctcgttcca aacgggtcct cactcgttct cgactagcaa ctcggctaga
    20941 cgcgtccagc ctcgagattg accgggcggt acgcacgacc ctgcacaacg ccttgagcat
    21001 ttacgaggtc gacgagccgg cactcgcaga gctcgctccc gaggtgatca ttactcaaga
    21061 cctgtgcgac gtctgcgcgg tggcccttgg agatgttcga agtgccgtag cccggctggc
    21121 gcagcgcgat cacatcgaga tcgtgtgcct cgcgcctaca ctgctcacgc aagtttggga
    21181 cgatgtctcc aagatcgccg acgccattgg cgagtcggcg tcaggacgcc gactgcgtgc
    21241 caacctcgag cggcgcgtct ccgaaatcgc gcagcgcgcg cactcttccg cggttcgccc
    21301 gcgcgtggct tccatcgagt ggctggaacc cctcatgctg ggtggcttgt ggatgcctga
    21361 gctcatcgag ttggcgggtg gcgttccggt cggagtcgaa gcgggccaac cggcccccac
    21421 cgtggggaca tccgagcttt gcgcgttgaa gccggatgtc atcgtcctca agccctgcgg
    21481 attctctttg cagcgggcgc tcgatgagcg ggcccccctg gagcgactac ggcgagagct
    21541 agggccgagc acacgcttgt tcgtgaccga cggcaatgct ttcttcaacc gccctggtcc
    21601 gcgtttggtc gagtccctcg agatcttggc cgcgtgtctt catccgaagc tttttgcgga
    21661 tctcgccgag cgtcatcgcg cggtaatcgt cgagtgcccg tgatcgctgg agcggtgact
    21721 cgcctcgccc ctcaactgcg cgtcgaggcc accgccaact ctggagtgga gacactgcgc
    21781 cctccacgct gagaggttgc cgccggatcg atcagcaaac gagcggcctc gcgcaaccca
    21841 tacacgaccg cgctcgcgcc cgcttctctg agccgcgccg ccgcttcccg cgagcgcttg
    21901 ttgcgagcct cggggagcag cacgatcagt cgtacgtcgg gcaattcggc gcgcagcctc
    21961 cggcacaaaa agcgagcgtt cgcgttgccg ctcggaggca gagccgcgat acagatcgcg
    22021 ctcggctgat gctgaatcgc gtctgccaac aacttggcac gcccggcccg cggtagcggc
    22081 actacgcgcg gtaaatcttc gcccagcgtc atggtcagca tccgcacgag cagcacgtcc
    22141 gactcggatt ctgcggggat cgccacaatc gtaccccggc gatcgcccgc cgcgtcctcg
    22201 cgcgtgcgcg ccaggtcatc gagagcggta gctttggcgt tggttgcaag cgcagccttg
    22261 agtccttcgt cggcgaccag tcggtccacg atatcgttga tgtcccttac cagacgctcg
    22321 gatgttcctt gcgccaggcc ctgcaagttc tgctcactca ccatcaagcc cagagcgggt
    22381 agaaagagct catcgaaggt atccggcaac gaggttgcca aagcacggcg acgcaccagg
    22441 cgataggcat ctttggaggc cttcgccaag gtacgttgat aaaactcgac ctccgcgttc
    22501 agaacgggtt cgtccccgag cgccacgctc aagaaagaga gctgtggaac gtgccgtccg
    22561 acgacggccg cgcagaccgt caaaggcgtg gccagcacca agcccaaagg cccccacagc
    22621 caagtccaga acagagccga gatgagcagg gccagcgccg agacaccagt gcgcttgccc
    22681 agcaccattg gctccagaaa gttcgcgacc agggtgtcca cgaccaggaa caatccaatc
    22741 gtcaacaaga cctcgcgcca acccgggaag atagcgaagg ccgccaatga cgggagtaaa
    22801 gttgcgatca ctgcgccgag cagcggcacg aagcggagca cccccgccaa cactccccaa
    22861 agcagcgcgt acggcactcc aatgaagtaa aggccgcccg caatcacgac accgaagccg
    22921 acattgatgt aggcctgggt caacagcaga tgccccaccc gcttgaccgc ttcggccatc
    22981 gttcgagtgg tgacggtcac gttctgcgta cccatcagct ggatcagccg cccacgcagg
    23041 tcctcgcggt ggccgagcac gaagatcgcc atcacgatca ccagtacgga gatccccaca
    23101 ggtcgcaaca acggcccgag cgcgtgctcc agccgttcga aggtcgagaa cccgcccgag
    23161 acgacttgga ccggagtaac gtcctcagcg cggatcacct gcgggtccag gctcttgccg
    23221 agctggttga tggctttggt caggtcttcc actaccccga agcgatcgct gcgcacgcgt
    23281 gtgagcttct cgctcatttg cgcggaatag cgctgcatct gccgcccgag atcggcggct
    23341 tgcgagatca agaccgtacc gaacacggcc accgcggcca gtgcggctgc cacgaccgag
    23401 gtcaccgaaa gaccgcgggg caccccgatg cgctcgagcg catccaccgg aggcgtgagg
    23461 acgaaagcca aatagaacgc cagcgcaacc ggcacgagca cgggctgacc cagcgccaga
    23521 atggcgacca ccagcacgaa gtcagccaga ggccctaagc tcagattccg cacagctcgc
    23581 tgatccggtt gaggagcatc gaaaagtcgg tcggtttctc gaaggcgtcg tcgacttgca
    23641 gcttcccgcg ctgctccgcg gacataccgc acaagacgac aatcggaatc gagcgccatg
    23701 ccgcagtccc tttcagcgtc tcgcacagat cccagccgct catcaccggc atcatcaggt
    23761 ctgtgaggac gagatctgga tccaatccgc cctcgagcaa ctcgatggct tgcgccccgt
    23821 tctcggcctc cgcggcgaag tacccttcgg tctcgagccg cctcagcagc gcatcgcgac
    23881 tatccgcatc gtcgtcgacc accagcacct tgcagccatt tctcgccaag tttcaccagt
    23941 cggttgcacc agtctgagca gtccgctgca agacacgcgc cgagctgcca cgtccctgaa
    24001 atgccaaggt cctttgactg ctgcgtcact ttacgcgcct cactgaggcg aatttggcgc
    24061 caaccgagcg cttctgcccc agggtctacc caatctagat cggaaatatc cagtttacgc
    24121 gaatgcattg cgtgtcttcg aacagcccgt gagcgccgtt tcccagcttc gactcgaaga
    24181 ctgttcgaag cggcgtggct tagcggcgac cttctatgcc ctgatcgacg ccggcctcgg
    24241 agtcaccgtg ggtcgccatg caaatttgca tgggttcagt tagcgtacac catgttcgat
    24301 tgggatgacg tccgctactt cttggccgtg gctcgcagcg gctcattgtc cgccgctgcg
    24361 cgcgttttgc aggttgcgca accgacggtc gggcgacgta tcaccgcatt cgagcggcgc
    24421 ctcggggcgg agctcttcga acgcaccctc gaaggccaaa agctcaccgc aacgggtcgc
    24481 cgcgtgttgg cgaccgccga gcgcatggaa gaagaggcgc tcgcgctcga acagcaaacc
    24541 gggcgagatc ccaccctgcg cggacgggtt cgaatcacgg cgtccgagtg gctcatccag
    24601 cgactattgg ctcccatctt ggtgccattc aatcgtgcac acccggagct cgaactcgag
    24661 ctctgggcgg acatacgcca cctgagcctg acccgacgcg aagcggacat cgctgttcgc
    24721 ccatcccctt tcgaacaagg gagtatcgta cagcgcgtcg tggccgagct caaattcggc
    24781 ctctacgcct cggaccacta cttgctcgaa cggggtgtgc ccgatttttc gcgagcctgc
    24841 gaaggccacg tcctgattgc catgagcgaa tcactaggca agatccctga cgtggagttc
    24901 ttgcctcgcg tcgcggcgaa ggctcgcatt gcaactcgga cgaacgggcg ggaacccatg
    24961 gccgtgctcg ccgcagcagg gctgggcatc gcctgtctcc ctcgcttcct tggggacgcc
    25021 actgcgggcc tccgccacct gccctgcccc gagccagagc ccacgcgccg actttacgtc
    25081 ggcttcctcg gagacgatcg tgccgcttca cgcatccaaa gctgcgtcac cttcctcgca
    25141 cacacgattc cgcgcttgcg ttccgcgcta gctccgagct gagttctaag aacttatgcc
    25201 agtaattgga acaggttcta agccggctgt tgtgccggca cccggtctgg ggcggctacc
    25261 ggtgaacgaa tcgatctgcc gctcaggcgc tcgaactcca cgacgtcgtg cgcgcagaag
    25321 atcgtgaccg cgggctgcgc cgcgtgcagc tcgcgcaggc gtctttgatt gagcagccgc
    25381 aaagagcgat ccttctccat catcgcttga tagaggcgga gcccgggcgt gcaatagggc
    25441 cgatccggat ccatttcggc atggtagaag taggcatccg ccgcgtagaa cagccaatcc
    25501 tgctcgcgtc ggacggcaat cccagcgtgg cccaaggtat gcccgatcag gggaaccatc
    25561 aaaagatctg gacccacccc gggcagctcc cgaacccccg aaaagccgaa ccaggtctcg
    25621 cccgcaccgg gctccagcag gttccacatg ctctgggtgt gccactgctg cgggcgatag
    25681 cgcatgcgat cgagcagcgt acgctgagcg cgcgccgctc ccgcctcgtc cccgaacagg
    25741 tgtaccgtcg cgttcgggaa atcatccagc cccccggcgt ggtcgaagtc gaggtgcgaa
    25801 agcgcgatgt gttgcacgtc acgcgcgccg aagccaagcc gctcgatttg ccgaatcgcc
    25861 gtcatttccg cccgcagctc cgggcgcatc aacgccagaa aaagcggatt cagccgcgaa
    25921 cgtggattct ccacatcgcg caatccatac ccagtatcta ccagtaccag gcaattcggc
    25981 gtctccacca gcaagcagtg cgagaccagt ttgccgcgta acgaaccgtg gctgaaacca
    26041 tccatcaacg cgccgcccag tgggcaggcc gacacgcaat tgaggtgatg aatcctcatt
    26101 cgagatagct ggacagtatc catttttcgt cacccgattg aaaagccacc aacacgcgcg
    26161 aggtgtcctg gcgcagcacc gagccgcgac cgaagcgcgc gtggcgcact acggctccca
    26221 cgtcgaactt cggtctggca ttcgtggcat cggactccgt gttggcggca gagtccgtga
    26281 gctgagactt cggggcatcc gactcaacga gctcgcagcc gcgagccagg gcggtcgccg
    26341 gttgatcacg acagttgtcg cagtgcccac aatccgacgc tgactcattt ccgaagtagc
    26401 gctgcagtac cttccaacgg cactcggtag tttgcgcata gtgcatgatg gcttcgatgc
    26461 gctctcgatc ctccgcgtgc cgcgcgtcgt gctcgttcac caatcgttcg atttcgccag
    26521 gcccccgcac cacgcgctcc tgctcgaact tcagcccgac tcgcctgatg agccttgctc
    26581 gctcgagctg cgccaggatc actttgacgc ggttcttcgg caagtccgcc acggcgcgga
    26641 tccgatccag cgtgagctgc actcgaccgt gctccgcgcc cagcgtttcc agcgctcgca
    26701 acacacccat cacctgctga cccgtagggt gtttcccgcc gagaaagtag ctctgaaccc
    26761 gcttgtcctc caggcgatac aacaagctcg ctcggcaagg ctccccgtcg cgaccggctc
    26821 gcccggcttc ttgatagtag gcctcgagag agtccggaaa ctcgtagtga atcacgtgcc
    26881 gcacgttcgg cttgtctacg cccaggccga acgcgttggt cgccaccatg gtctcgattt
    26941 catccgccat gaaggcgcgt tgcacgcgtt gccgctcctt ggtcggcagc tttccgtggt
    27001 agcgccccgc ggacactccc gcctgcgtca gccactcgaa aacctgctcc gtgtggcgca
    27061 cggtcgccgc atacacgatc ttggggccag cttcgccgcg gaccagctcg atcacccgct
    27121 cctgcttggc cgcctcactc ggcgtgcgat gcacctccaa gaacagattc tcgcggtcga
    27181 tcccggtgtt gatcacgcgc ggcgagcgca atccgagctg ttcggtgatg tcggccacta
    27241 tcgccggtgt cgccgtcgca gtgagcgcca ataccggcgg ccctccgagc gcctcgatcg
    27301 ccgttcgcaa ggccaggtat gccgggcgga aatcgtgccc ccaacgcgac acacaatggg
    27361 cttcgtcgat cacgacgagc gacgctcccg cccgcccgag cagctgtaga tattcagcgt
    27421 tctcgagccg ctccggggtg acgtaaacca agtcatggga gccccgcttc acctcgtcgc
    27481 gaacctcccg ctcttcgggg gcactcagcg tggagtccac ctttgcggac gcgatctgca
    27541 atgcttgtgt cttctcctgt tgatcctgca tcagagcaat caacggtgag acgaccagcg
    27601 taggcttcgg gaggaacaac gccggcaact gaaagcaaag ggacttcccc gctcccgttg
    27661 gcaaaacgcc cacggcgtcc cgccccgcga gcacgcacct cagcaggtcg agctgcccgg
    27721 gccggaagct cgtcaccccg aagcgccggc gcgcctcacg cctcaactca ctcaacccga
    27781 ttttgtttgc tccttccggc gcgccctgtt tcatcattcg ttccgctcgt tcgatgctct
    27841 ccgctgctgg catccacgag caagaaccgt tctcgtgggg ggccggcgag gtgccgcgac
    27901 cggcggagcg cgttcgctag aacgccacgc ccgccgcgcg aagggcagac gcattcgaac
    27961 tcagtgggat gaagaggata gctctcgact cggcccggcc ctatgttggc ggggcaatgg
    28021 cctcaccgct cgtttcctcc tccggcttcg atctcacgcc ccccagtgaa actcaacgcg
    28081 aacggagaac ctcggagctc agtcccgaac aagcccacgt tctcttgcgc cacggcacgg
    28141 aggcaccatt ttgcggcggt ctacttgggc aaaagagtcc aggcacttac tgttgcggct
    28201 tctgcggcct gcccctgttc cgacaagaaa ccaaattcga aagcgggacg gggtggccca
    28261 gcttctatgc ccccttcgat ccatctcatg tcaaagaagt ccgtgacacg agctacggaa
    28321 tgctgcgcgt cgaaacgcgc tgtgctcgct gcgactccca tcaaggccat gtgttcccag
    28381 acggccctcc cccgactcgc ttacgctatt gcatcaactc ggtggccttg aggttcgtgg
    28441 cagaagggga acccattccc aatctcctga aatagcgagc gagcgtccgc ccctcacgaa
    28501 tgcgtcaacg gctttggtcc agcagctttt cggtggccac tccgtaaaga gcgcggggcc
    28561 cgtcgaatgg cgcgtcagcg cagccgcgtc cctcacgtgg caatctccac cgcgccggcc
    28621 cctatccgat cggcccccgc tgtcgagacc atgcagagag ctgtgccgct tgcgcggttt
    28681 tgcgcggtac tgcgtgggac gaggctgcgc aaaagacgaa gcgtgacgca cggcgcgccc
    28741 gtaatctcgg gcggccaatt caccctcctc gagtggattg catggtgcga ggctgccccc
    28801 tgtagccgct caatttcgag aactaacgaa aagcgacgac caca
//
LOCUS       DASTKU010001051        15739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_207570, whole genome shotgun sequence.
ACCESSION   DASTKU010001051 DASTKU010000000
VERSION     DASTKU010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 15739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15739
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207570"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1497)
                     /locus_tag="VFQ61_37370"
     CDS             complement(43..1497)
                     /locus_tag="VFQ61_37370"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HET9960239.1"
                     /translation="MSSPLSAGLPDLASDASSGAKSSSATLSPSLLVQALREALREAR
                     PRQGRARFHVLNVAAGETGIAGHAWLRLEGIGSASFLSDPTGYEQSAIEQVEVLTAAG
                     PERFQSLATQAQALFEQIDDPSESLRLIGGFAFQPARASSELFRNFGEARFVLPRLIH
                     ERRDGQARLSLVVRDQELADAMGQANLLSFVERVLQHATKPRRMLQAGGEDPSDASSP
                     RVDAGSEADFTALVERSRDAILRRELEKVVLARRVDVELPDGVEPDVVLERLAASAPE
                     CIRYAFMVAGRTFLGATPERLVRKSGGSFETEAVAGSIPINELSALRLLESTKDRAEQ
                     AIVVREVLQSLRPLCATLEPAETPEIHRLRHVAHLRTRIRGKLLRPEHVLELVARLHP
                     TPAVGGFPSARAQAWIAEHEPAERGWYSGPIGWFDRHGDGAFMVALRCGVLHGRHAAL
                     FAGAGIVEGSKPESELQETRWKLQGLLGALGVTA"
     gene            complement(1494..2231)
                     /gene="ubiE"
                     /locus_tag="VFQ61_37375"
     CDS             complement(1494..2231)
                     /gene="ubiE"
                     /locus_tag="VFQ61_37375"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HET9960240.1"
                     /translation="MTTNDMSLQNPNAARQGSGRMFDAIAGRYDLLNRVLSFGMDQSW
                     RRKTARALNLGSKARVLDLATGTADLALQVVREAPDCHVVGLDPSINMLEVGRRKVAE
                     LGLSGSIELLEGDAQQLPFGDAEFDGVCIAFGIRNVPDRSRALREMARVTRPNGRIAI
                     LELSEPEGGLLGAIARFHVHSVVPHLGALLSGASEYRYLQRSIAAFPPAREFQQVISD
                     AGIEPLELIPLRFGVCHLYVGKPRALA"
     gene            complement(2228..2764)
                     /locus_tag="VFQ61_37380"
     CDS             complement(2228..2764)
                     /locus_tag="VFQ61_37380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960241.1"
                     /translation="MTENQRTSSEDPELRVADHIGRLMEFWGFKRPMGRIWALLYLSD
                     EPLGAAEIGDQLRMSTGAVSMALSELVKWGAVRKTWKPGERRDFYESETSIGRLVQRV
                     IRERELLLVQEFAETLDAAERKLSEDSAMSNERATFKRERLRQLGALAKLGESLLSAL
                     VSGRTVDPAPFLKTAERR"
     gene            3082..4440
                     /locus_tag="VFQ61_37385"
     CDS             3082..4440
                     /locus_tag="VFQ61_37385"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET9960242.1"
                     /translation="MEGAQTWRVAVVGAGPAGFYATDYLLKAGAKVDLFERLPAPFGL
                     LRYGVAPDHQNIKRAGVALERTLKQPDLTFIGNVSLGKDVSLEELLADYDQVLVATGS
                     AGDRRMGIPGESLPGSVAATSFVGWYNAHPDFSVQDFDLRGSRAVVVGVGNVAMDVAR
                     VLVRSPADLAKTDIANYALEILSRSPIREVVLLGRRGPAQAAFDVGELKDIAALPGVE
                     LVLDGRTSLEFDGDPAQLSTSARRNLEFLATLPRETSGTRERVVRLVFCASPKALYGN
                     AEGVQEIEIERTRLRRTEDGSVVAEGTGELERLETNFVVRAIGYRAEPVSGLPFDSRA
                     SLIPNQGGRAGQPHEIVPRCYVSGWIKRGPVGLLGSNKVDARETVDHMLEDRDAALAS
                     QGARRPGSVRARLEERGARVVDAAAWQRVDEKERSAGSSAQKVREKFATVGELLRAAH
                     EG"
     gene            4496..5134
                     /locus_tag="VFQ61_37390"
     CDS             4496..5134
                     /locus_tag="VFQ61_37390"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HET9960243.1"
                     /translation="MSTRGSRLLFRVLFALSVGFSQSLSPTLAEAQQPGAAADADHGA
                     HVAPPTNFNQFVPGAAELEGRIIAPCCWNQTIDIHGSEVSTSLRQEIRSRLLSGENPE
                     AIQLSLVERYGPRILAVPPGSRLSSAGILLSLAMGVGGVFAMVLLRRWQRRSTDQALE
                     SGAPGNEGEGTKGATPATRKAGSASNNSPEGTQQRARLDAEIDAELAKLDAD"
     gene            5261..6331
                     /locus_tag="VFQ61_37395"
     CDS             5261..6331
                     /locus_tag="VFQ61_37395"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HET9960244.1"
                     /translation="MLELPLLTVSGTARQMGRAQGEALRAQIGAFVDQRLSALRGYLE
                     ERKQPALFERFLATGQECLGLAQRFDAEAIEEHDGIAEAAGVDRALLYAATNMTDLRD
                     VLLLPKSPDREGCTSLLVPGARTRSGQLLAAQTWDLNPTDLDFVVAIQRRPLAGPQTW
                     AITCTGALSLIGMNEWGVAVGTTNIKTRASRAGIGYLSVLHRAIRTRSRVEAQKLVQT
                     APLAAAHTYWLADVQGACELECDPDTRSARELTLEPIVQTNHCQADVLSAREGEPPTS
                     SSRARLARALNELARGNVDLKRVQELFADRSDGVDSINRYVEDDQGTSTNACIVCSPT
                     ERKLWACRGSADRGRWEELSFS"
     gene            complement(6391..8472)
                     /locus_tag="VFQ61_37400"
     CDS             complement(6391..8472)
                     /locus_tag="VFQ61_37400"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET9960245.1"
                     /translation="MAELNPAQNEAVHTLSGPLLVLAGAGTGKTRVITFRIAQLIRTG
                     ITPSRILAVTFTNKAAREMRERAQKLLGRRDRNTKPPEISTFHSLCVRVLRRHGRKLG
                     YPEAFSIYDRGDQETLIRNALREIRVSSGTLRPGDVLAFIGNWKNQGVTPDLALTQAE
                     GNRAELAASAYERYQAQLKAIGAMDFDDLLLNVNKLFREHPDARFAEASRYDHLLIDE
                     YQDTNDLQYSIVKALADRHRNLCVVGDDDQSIYGWRGAEIAHILGFQKDWPEAKVVRL
                     EANYRSRAPILELANTLIAHNSARHQKVLRPSREGGPAPRILRMEDEAMEAENVVQQI
                     QQLLIGDTSADASQRVSPSDIAILFRTNEQPRAFELELRRAKIPYMMIGGQSFYDRRE
                     IRDVLSYLRVLANPQDEVSLLRIINTPARGIGTSTIEALLEHAVKAGQPLWSVLPEAI
                     ANAGLSDAIAARVENFRRMIEGFRAKVNTIPIVDLARELLTTVDYRTELERVHTAPGE
                     AEARYQMVEELVNSAGLYESREENPTLLGFLEQAALADRDDFGKDDKKERHALTLMTL
                     HSAKGLEFPHVYMVGMEEGILPHQRSILEGRGVDEERRLCYVGVTRAQETLTLTFAKA
                     RMKWGKPRPSIPSRFLMEMRGDTERAKRAAEAAEALYRSNPDGKTKPEIEDAERAPKK
                     ARASKAAQRSR"
     gene            8604..9389
                     /locus_tag="VFQ61_37405"
     CDS             8604..9389
                     /locus_tag="VFQ61_37405"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9960246.1"
                     /translation="MYTETVGSGPALVLAHGFGGSARNFRPQARALGQELTVWTYDAR
                     GHARSKLDASQSGSESDDSYDTAALVRDFGEVVTRAGQPVIAGGLSMGAYTALTYTLN
                     ARHAVRGLILSAFPAPGADRQRAWALGFARSIREHGLAAAGEEFAWGDRSRFDERSRA
                     LIKQGFLEHDPLALAALLEHVLAELPAPDAMATDLAGLRTPTLVVVGAEDEESLRPCE
                     RLAELLPNARLSVIPGAGHVLNLSHATEFNAAVRGFIAELPTT"
     gene            complement(9521..10141)
                     /locus_tag="VFQ61_37410"
     CDS             complement(9521..10141)
                     /locus_tag="VFQ61_37410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960247.1"
                     /translation="MTLGGSVIEAQGGGGYGPPPGGYPPGGWGPPGGGPPGGGPPGGG
                     PPGAPPGGGGYGQPPGGYGPPPGGYGPGGGGFGQPPGGGYGQPPGGFGPPPGGFGPPP
                     GPGGFGPYPGGPGPNAELKKQATTWLIVAGVTFFFCGNCFGLFGAIFLFLAMQAADQG
                     NSAEAESKLKLGKILTIVGLALGVVLGILWAVLNVIGIAGSAIFNS"
     gene            10464..11417
                     /locus_tag="VFQ61_37415"
     CDS             10464..11417
                     /locus_tag="VFQ61_37415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960248.1"
                     /translation="MRGESMNHERECREAEDATHQRGGDCARARMMAFVAVVGIVAGC
                     RARQDAEHRAPEPVRSASYLRGDRVLVEARAAEFFEARVLTQQGDRLRLLRASGEDDL
                     WVSRADVYRLPPESKGHAGPWAICSNEIEPGSGPSWIGCRITKESGDRLNVEMLDGRS
                     LTLANRSVLRASPLTEMNLRRAFEQARARRDFLDRAAHAGRPHVPTGFRALPRMRVLA
                     LRRGGWYLGTVRELHKDGVDVAFESKTQYERIPAADLVPEPPMSEAPLRGSFALVRPL
                     SPFDAWPVLRVAGVSDREFRVVNESGEERVVAPRDIVPLFP"
     gene            complement(11471..13141)
                     /locus_tag="VFQ61_37420"
     CDS             complement(11471..13141)
                     /locus_tag="VFQ61_37420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960249.1"
                     /translation="MSGELANIARVLVAAGLLVCACRTAKPAPCANETKTARREDAPS
                     LAARIHALSAQSRVLATEPGSVGDRVSALLSVPSESCAVMIARAGPSIEDLDLFAYGE
                     DGSVLGSDEGPDTVPGLLVCPPHPTRIWIAARIAAGYGLVALGAQIVEPKLATQVAAA
                     LGVRSDGPKRTGVPVDLETALAEHRQEIGGEWQDQRRSSVPLDARLPTRVSATIDAER
                     CLDAVLVPSNEVGHLELNALDESGAILGRARAMGRNRALVVCASEPAQVTFELRPLSG
                     RGPGWLTLSQSKPGSESSIDSEIARVEVYPSGSLEEEQTRLRQQLAREHGYANAQRVL
                     SGQAEVGRLSSSALHLKAGCTRLDLIGARPLRGLQAFLLNDALEIEAQQAASSQLTFF
                     TCSAHPRSARLDLEARLRAGPFALWASGEPDVPANLMNAPLAANRLLESMVSRGVLRR
                     ASEIGQVRTFDMSDSRIENLDLTVPFGRCMDVTLALGAGASGAEIRLVGSDGAQVALG
                     RGSQVGTARVCALSADSAENRLKTRAELRVIAGSTQALVATRMLSPSR"
     gene            complement(13138..14499)
                     /locus_tag="VFQ61_37425"
     CDS             complement(13138..14499)
                     /locus_tag="VFQ61_37425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960250.1"
                     /translation="MSLGRALRLIVDASGAVLFGLLFAQPASADLNADAELLAARGRA
                     PNHVFRLPPRLGERSSPLVIFLPEAVFSGLPSSCVSVAVLGPMTAHFTLRAVGATSMP
                     ELPDLPDRGVVSSLAGLAELVYCGARKTKLAALVVELRSPRAVLETIVVESATPVPPA
                     TDSLPHRDPGPLEPAPLLNTTALAPIAQRLGTREQRAAREGLRWEAPHKLTRLGAFGG
                     SYAERVEPGCHEFDFLLDLPAHPVGSTEGRSTSEALARGESSQLSVEPELSPGQAIYE
                     RQRGEGALSHFGFCAGEAGRATFNVSFAPRQAEVWVTHARSELPAHLPDQLGAVGTAA
                     WAALLRRHATRVGSDPIDVAMGVSGPTAMPAQIEPGACYVAGLVRLAGNAQTLAIAAQ
                     VGRAWTRNRSRDELGALIAFCAGEQRSMRVETDARGGGVVWMYSLWQTSRRPLGSSLE
                     LAP"
     gene            complement(14496..15008)
                     /locus_tag="VFQ61_37430"
     CDS             complement(14496..15008)
                     /locus_tag="VFQ61_37430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HET9960251.1"
                     /translation="MDQPWIHGGIRIPASVLAEVERAGREAYVRDEEACGYLEGPAHD
                     PLGVDRAIELENLANKYHKVDPEGHPRTGREYFKINSLKFERALSEGAARGRPVKVFF
                     HSHLDCGSYFSAEDAASMTLGGTGEPSYALSYLVTAVDKGEVTQHRLFVWDPASRSFV
                     EAPFTRVEEA"
     gene            15289..>15739
                     /locus_tag="VFQ61_37435"
     CDS             15289..>15739
                     /locus_tag="VFQ61_37435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960252.1"
                     /translation="MLHFSVIRLLAYLLLAVFVTPAYAAPRKKPVKPEAVNLDEEEAQ
                     EAPKKQEAPKKETPKPAAPKATPKPPPKVETVDVEEEDGEGEGSGDEEEDAGQQTAGL
                     ECTPAVSEIETRRPIPISCAPTKKGIERVEVRYRAPARKKWTKLYLRK"
BASE COUNT         2835 a         5149 c         5022 g         2733 t
ORIGIN      
        1 tcgagagcaa cagctgtgcc cagcgcgtca gcgtggcgtc cgtcacgcag taactccgag
       61 cgcccccagg aggccctgca gcttccagcg cgtctcttgc agttcactct cgggcttgga
      121 gccttccacg atcccagccc cggcgaacaa cgcggcgtgt ctcccgtgaa gaacgccaca
      181 ccgcaatgcg accatgaagg cgccgtcgcc gtgccgatcg aaccaaccga ttgggcccga
      241 gtaccagccg cgctcggccg gctcgtgctc ggcgatccag gcttgggcac gagcggaagg
      301 gaacccaccg acggccgggg tggggtgtag ccgcgccacg agctccagca cgtgctcggg
      361 gcgcagcagc ttgcctcgaa tgcgcgtacg aaggtgcgcg acgtgacgca agcgatggat
      421 ctccggagtt tcggccggtt ccaaggtagc gcatagcggc cgcaggctct gcagcacttc
      481 gcgcacgacg atcgcctgct ctgcgcgatc tttggtgctc tcgagcaaac gcagcgcgga
      541 gagctcgttg atgggaatgg acccagccac cgcttcggtc tcgaagctcc caccgctctt
      601 tcgcaccagc cgctccggcg tggctccgag gaaggtccgg cctgccacca tgaacgcata
      661 gcgaatgcat tcgggggcgg aggcggcgag tcgctccaag accacgtccg gttccacccc
      721 gtcgggcagc tcgacgtcga cacgacgcgc gagcacgact ttctcgagct cgcggcgaag
      781 gatcgcatca cgactccgct ccaccagcgc cgtaaagtcc gcttcggagc cggcgtccac
      841 gcgcggtgag ctggcgtccg acggatcttc cccgcccgcc tggagcattc ggcgcggctt
      901 ggtcgcgtgc tgaaggacac gctccacgaa ggagagcagg ttcgcctgac ccatcgcatc
      961 ggcaagctcc tggtcgcgca cgaccaagct caaccgcgct tgcccgtcgc gccgctcgtg
     1021 aatgagccgc gggagcacga aacgcgcctc tccgaagttg cggaagagct ccgaactagc
     1081 gcgcgcaggt tggaacgcaa agccgccaat cagccgcaag ctttcgctcg gatcatcgat
     1141 ttgctcgaac agagcctgag cttgcgtggc gagcgactga aaacgctctg ggcccgccgc
     1201 ggtcagaact tcgacttgct cgatcgccga ctgctcgtaa cccgtcggat ccgacaagaa
     1261 cgaggccgag ccgatgccct cgaggcgaag ccaggcatgt ccggcgatcc cggtctcacc
     1321 agccgcgaca ttgagcacat ggaagcgcgc tcgtccctgg cgagggcgcg cttcgcgcaa
     1381 agcctcccgc aaggcttgca ccaacagcga aggggagagc gtcgcggacg atgacttggc
     1441 gccgctgctc gcatccgacg ccaagtcagg aagaccggca gagaggggcg aactcatgcc
     1501 aaggcccgag gcttacccac gtacaggtga catacgccga aacgcaacgg gatgagctcg
     1561 agcggctcga tccccgcgtc agagatcacc tgttgaaact cgcgtgccgg tgggaacgcc
     1621 gcgatcgaac gctgcagata acgatactcg gatgctccgg acagcagcgc tccgaggtga
     1681 ggcaccaccg agtgtacgtg gaagcgggcg atcgcgccca gcaagccgcc ttctggctcg
     1741 gacagttcga ggatcgcgat tcgcccgttg ggccgcgtca cccgcgccat ctctcgcagg
     1801 gcgcgggagc ggtctggaac gttgcggatg ccgaaggcga tacacacacc gtcgaactcc
     1861 gcgtcaccga agggcagctg ctgcgcgtcc ccctcgagca gctcgatcga accgctcagg
     1921 ccgagctccg caaccttgcg ccgcccgacc tcgagcatgt tgatcgaagg atccaagccc
     1981 acgacgtggc aatccggtgc ctcgcgcacc acctggaggg cgagatcggc ggtaccagta
     2041 gctaagtcga gaacgcgagc cttcgacccg aggttcagcg cgcgcgcggt cttgcgtcgc
     2101 cagctctgat ccataccgaa cgagagcact cggttcaaaa ggtcataacg gcccgcgatc
     2161 gcatcgaaca tgcggcccga gccttggcgt gccgcgttcg gattttggag actcatgtcg
     2221 ttcgttgtca tcgtcgttca gccgtcttca gaaacggtgc ggggtcgacg gttcgccccg
     2281 agaccaaagc agaaagcagg ctctcaccga gcttcgcgag cgcgcccagc tggcgtaggc
     2341 gctctcgctt gaaagttgcg cgctcattac tcatcgcgct gtcttcggac agctttcgct
     2401 ctgcggcgtc cagagtttca gcgaactcct gcacgagcag aagttctcgc tcccgaatca
     2461 cccgctgtac gagacgacca atgctcgtct cggactcgta gaaatcgcgc cgctcgccgg
     2521 gtttccaggt ttttcttacc gcgccccact tcaccagctc cgagagcgcc atgctcaccg
     2581 cgcctgtgct catgcgcagc tgatcgccaa tctcggccgc ccccagcggt tcgtccgaca
     2641 agtacaacag agcccagatg cgacccatag gtcgcttgaa gccccagaac tccatcaaac
     2701 gtccgatatg atcggccacg cgaagctctg gatcctcgct gctggtgcgc tggttctcag
     2761 tcatcggcag gccttcatag gtagagggat cgcgcacctt gtcgcctcac gggcggagcc
     2821 ctctcacgag cgggcggcgc ggagcgagcg tacgtgatgt ttgagctttc aaaatctctt
     2881 gaaactttag gctcgaggtc cagtggggtc aagccattct ccggcaaaaa tcagtaaggt
     2941 ttcggcgtgg tgtctatccg tccacggggg atgctagggt cccggtccag gagacgtcac
     3001 gaaatggcag gctcagaccg cgctcagatg actgaacgcc aatctccctc tgaagccggc
     3061 cgctccggtg ggagcagctc gatggaaggt gctcaaacct ggagggtggc cgtggtcggc
     3121 gcgggaccgg cgggctttta cgccacagat tacctgctga aggcgggagc aaaggtggac
     3181 ctattcgagc gtctccccgc acccttcggg ctcttgaggt atggcgtcgc gcctgatcac
     3241 cagaacatca agcgcgcggg cgtggcgctc gaaaggacgc tcaagcagcc ggacttgacc
     3301 ttcatcggca acgtgagcct cgggaaagac gtgagcctcg aggagctgct cgccgactac
     3361 gatcaagtgt tggtcgcgac cggatccgcc ggggaccggc gcatgggtat acccggtgaa
     3421 tctctgcctg gcagcgttgc cgccacgagt ttcgtcggct ggtacaacgc gcatccagac
     3481 ttctccgttc aagatttcga cctgcggggc tcgcgggcgg tcgtcgtggg ggttggcaat
     3541 gtcgccatgg atgtcgcgcg cgtcctggtt cgaagtcccg ccgacctggc caagacggat
     3601 atcgcgaatt acgccttgga aattctgagt cgcagcccga ttcgcgaggt ggtcctactc
     3661 ggccgacgcg gtccagcgca ggcggctttc gatgtgggag agctcaagga cattgcggcg
     3721 ctacccgggg tcgagctcgt gctcgatggg agaacgagct tggaattcga tggcgatccg
     3781 gctcagcttt cgacgagcgc ccgacgcaat ctagagttct tggctacctt gccgcgggag
     3841 acgagcggca ccagggagcg agtggtgcga ctggtgttct gcgcctctcc gaaggccctg
     3901 tatggaaacg cggaaggcgt gcaggagatc gaaatcgagc gcacgcgtct gcgccgaacc
     3961 gaagacggca gcgtggtcgc cgaagggacg ggcgagctcg agcgcctcga gaccaacttc
     4021 gtggtgcggg ccattggtta ccgcgccgaa cccgtgagtg ggttgccttt cgatagtcgg
     4081 gcgtcgttga tccccaatca aggtgggcgc gccggtcaac cgcacgagat cgtgccccgt
     4141 tgttacgttt cgggctggat caagcgcggc ccggttgggc tgctgggctc gaacaaggtg
     4201 gacgcccgcg agacggtgga tcacatgctc gaagaccggg acgccgcttt ggcgagccaa
     4261 ggcgcacgac gccccggcag cgtgcgagct cgcttggaag agaggggagc cagagtggtc
     4321 gacgcggcgg cgtggcaacg agtcgacgaa aaggagcgct cggcaggcag ctccgcgcaa
     4381 aaagtccgtg agaaattcgc cacggtgggc gagttgctgc gggcggcgca cgagggctga
     4441 acgacgcgat acgttcgggg tgcccgacca cgaccgttgt ggcaagctcg aggccgtgag
     4501 tacccgaggc agccgtctgc ttttccgcgt cctgttcgcg ctgagcgtag gattttccca
     4561 atccctgagc ccgacgctgg ctgaagcaca acagcccggt gccgccgcgg atgccgatca
     4621 cggggctcac gtcgccccac ccaccaactt caaccagttc gtgcccggcg cggcggagct
     4681 cgaggggcgc attatcgcgc cttgttgctg gaatcagacg atcgacattc atggttccga
     4741 agtctcgacc agcctcaggc aagagatccg ctcgcggctg ctatccggcg agaaccccga
     4801 agcgatccaa ctctccttgg tcgagcgtta cgggccgcgc atcctcgcgg ttccgcccgg
     4861 cagccggctc tcgagcgctg ggattctctt gagcttggcg atgggcgtag gcggggtgtt
     4921 cgcgatggtg ctgctgcggc gttggcagcg acgatcgacg gatcaggcgc tcgaatccgg
     4981 cgctcccggc aatgaaggcg agggaacgaa gggggcaacc ccggctacgc gcaaggccgg
     5041 gtccgccagc aacaacagcc ccgagggtac gcaacagcgc gcgcgtctcg acgcggagat
     5101 cgacgcggaa ctcgcgaagc tggatgcgga ctaacgacgt ttggattcgg agtagtccgg
     5161 ggatgcgccc acggccgact ggcggcggct acatctacgc gaatcgtaag ttgagccacc
     5221 aactctgact ggctttgatt tctgactgga ttgccctctc atgctcgaat tgcctctttt
     5281 gaccgtttcc ggaaccgctc gccagatggg gcgggcccaa ggtgaagccc tgcgcgcgca
     5341 gatcggcgcc ttcgtggatc aacgcttgtc ggcacttcgc ggttacttgg aggagcgcaa
     5401 gcagccggcg ctgttcgagc gctttctggc caccgggcaa gagtgtttgg ggctcgcgca
     5461 gcgcttcgac gccgaagcaa tcgaggagca cgacggaatt gccgaagcag cgggggtgga
     5521 tcgcgcattg ctctatgcag cgacgaacat gaccgacttg cgtgacgtct tgttgttgcc
     5581 gaagagcccc gaccgcgaag gctgcacgag tttgttggtg ccaggcgcgc gcacgcgcag
     5641 cggtcaactc ttggccgcgc agacctggga tctcaacccg accgacttgg acttcgtagt
     5701 ggccatccag cggcggcccc tcgccgggcc ccagacttgg gccatcacct gcaccggcgc
     5761 gctgtcactg atcgggatga acgagtgggg cgtggccgtc gggacgacca acatcaagac
     5821 gcgcgcctcg cgcgcgggaa ttggctactt gtccgttcta caccgcgcca ttcgcacgcg
     5881 ctcacgggtg gaagcgcaga agctcgtcca gaccgcgccg ctcgcggccg cgcacacgta
     5941 ctggctggcg gacgtgcagg gagcctgcga actcgagtgc gatccggaca cgcgctcggc
     6001 gcgcgagctc acgctcgagc cgattgtgca aaccaaccat tgccaggccg acgtcctctc
     6061 cgctcgggaa ggcgagccgc cgacgagctc gagccgcgcg cggctcgcac gggcactgaa
     6121 cgagctcgcc cgcggcaacg tagacctgaa gcgcgttcag gagctgttcg ccgatcgctc
     6181 cgacggggtc gatagcatta accgctatgt ggaggatgac caggggacga gcacgaatgc
     6241 ttgtatcgtg tgctcgccca cggaacgcaa actttgggcc tgccgcggct ctgctgatcg
     6301 aggtcgctgg gaagagttgt ctttctccta gggcgagctt cttgagccca atccccacga
     6361 atgagtgacg ttcgtgcggt tgctgttcgc tcagcgcgaa cgttgcgccg ccttgctggc
     6421 gcgggccttc ttgggtgcac gttcggcgtc ctcgatttcc ggcttggttt ttccgtccgg
     6481 attcgagcga tagagcgcct ccgctgcctc ggccgcacgc ttggcgcgtt ccgtgtcacc
     6541 gcgcatctcc atcaggaatc ggcttggaat ggacggtctc ggcttacccc acttcatgcg
     6601 cgccttggcg aaagtgaggg ttagcgtttc ttgggcccgg gtgacgccga cgtaacacag
     6661 gcggcgctcc tcatcgactc cgcgcccttc caagatcgag cgctgatgcg gcaagatgcc
     6721 ttcctccatg ccgaccatgt agacgtgcgg aaactccaag cccttggcgc tgtggagcgt
     6781 catgagcgtc agcgcgtggc gctccttctt gtcgtccttg ccgaagtcgt cgcggtccgc
     6841 gagcgccgct tgctccaaga agccgagcag cgtcgggttc tcctcccgag attcgtacag
     6901 acccgccgag ttgacgagct cttcgaccat ctggtagcgc gcctcggctt caccgggtgc
     6961 ggtgtgcacg cgctcgagct cggtgcggta atccacggtc gtcagcagct cgcgcgccaa
     7021 gtcgacgatg ggaatcgtgt tcaccttcgc tcggaacccc tcgatcatgc gccgaaagtt
     7081 ctcgacgcgc gcggcgatcg cgtccgaaag ccccgcattc gcgatagcct cgggcaacac
     7141 ggaccacagc ggctggcccg ctttcacggc gtgttcgagc agggcttcga tggtagaagt
     7201 gccaatgcca cgagcgggcg tgttgatgat gcgcagcaac gaaacctcgt cctgcgggtt
     7261 ggcgagtacg cgcaagtacg agagcacgtc ccggatctcg cgccgatcat agaacgattg
     7321 tccgccgatc atcatgtacg ggatcttggc ccggcgcagc tcgagctcga aggcgcgggg
     7381 ttgctcgttg gtacgaaaca ggatcgcgat gtcgctcggg gagacgcgct gcgatgcgtc
     7441 cgcggacgta tccccgatca gcagctgttg aatctgctgc accacgttct ccgcctccat
     7501 ggcctcgtct tccatgcgta ggattcgggg cgccggaccg ccctcgcgcg agggcctcag
     7561 caccttttgg tgtcgtgcac tgttgtgcgc aatgagggtg tttgcgagct cgaggatcgg
     7621 cgctcgggag cgatagttcg cctccaagcg cacgactttg gcctctggcc aatccttctg
     7681 aaagcccaga atgtgggcga tttcggcgcc acgccagccg tagatcgact gatcgtcgtc
     7741 acccaccacg cataggtttc gatgccgatc ggcgagcgcc ttcacgatgc tgtactgcag
     7801 gtcgttcgta tcctggtact cgtcgattag caggtgatca tagcgggaag cttcggcgaa
     7861 gcgcgcgtct gggtgctctc gaaacagctt gttcacgttc agcagcaagt cgtcgaagtc
     7921 catcgcgccg atggccttca gctgcgcctg gtagcgttcg taggcggaag ccgcgagctc
     7981 ggcgcgattg ccctccgcct gagtgagtgc caaatccggc gttacgccct gattcttcca
     8041 attaccgatg aaggccagca cgtctcccgg gcgcagagtg cccgagctga cacgaatctc
     8101 gcgcagcgca ttgcggatca gagtctcctg atccccccgg tcgtagatcg aaaatgcctc
     8161 ggggtagccg agctttcgcc cgtgccggcg cagcactcgc acacacagcg agtggaacgt
     8221 cgagatctcc ggcggcttgg tgttgcgatc gcgacgcccg agcagcttct gcgcgcgctc
     8281 acgcatctcg cgcgcggctt tgttggtgaa ggtcacggcc aagatgcgcg agggcgtgat
     8341 gcccgttcta atcagctgcg cgattcggaa ggtgattaca cgcgtcttgc cggtacccgc
     8401 acctgcaagc accaacagcg gaccgctcaa cgtgtgcacg gcctcgttct gcgctgggtt
     8461 cagttccgcc acggcgctac cccttacgaa cgcggcggcg gctggtcaag ggttgacggg
     8521 cgcgcgctct gcgtagcgta aattcacgaa gaagcagccg gccggcgtgg cccgatcgcg
     8581 ggcctgatgt tagagacgcg cgcgtgtata cggaaacggt cggctccggt cccgcgctgg
     8641 tattggccca tggttttggc ggcagcgcgc gcaacttccg gccgcaggcg cgcgcgctcg
     8701 gacaggagct cacggtctgg acgtacgatg cgcgcggcca tgcccgcagc aagctcgatg
     8761 cgtctcagag cgggagtgag tccgacgact cttatgatac agcggcgttg gtccgcgact
     8821 tcggtgaggt ggtcacacgc gccgggcagc ccgtgatcgc gggcgggctg agcatgggtg
     8881 cttacacggc gctcacgtac acgctcaacg cgcggcacgc ggtgcgaggc ctgatcttgt
     8941 ctgccttccc agcaccgggc gccgatcggc aaagagcctg ggcgctcgga ttcgcgcgct
     9001 ccatccgtga gcacgggctc gctgccgccg gcgaagaatt cgcttggggg gatcgttcac
     9061 gattcgacga gcgctcccgc gcgctgatca aacagggctt tctggagcac gatccgctgg
     9121 cgctcgcggc tttgctcgag cacgttctgg cagagttacc cgcgccggat gcaatggcga
     9181 ccgatcttgc gggcctgcgc acgccaacct tggtcgtggt tggtgcagaa gacgaggagt
     9241 cgctgcgccc atgtgaacgg ctcgcggagc ttctgccgaa cgcgcgcctg agcgtgatcc
     9301 ccggggccgg ccacgttctg aatctatccc acgcgaccga gttcaacgcg gccgttcgcg
     9361 gcttcattgc cgaactcccc acgacctgag tagtcggaga gagccgtcac caaggccgcg
     9421 gcaaacaacg ccgcacgcgc gcgaaggagt gtcgttccag agctctggaa cgctccaaag
     9481 ctctggcgtc acaccgaggt ctatagcgcg agttgcgagc tcaagaattg aagattgccg
     9541 aacccgcaat cccgatcacg ttcaagaccg cccacaagat gccgagcacg acaccgaggg
     9601 ccagtccgac aatcgtcagg atcttaccaa gcttcagctt cgactcggcc tcggcgctgt
     9661 tgccctgatc ggccgcctgc atggcgagga acaggaagat ggctccgaac aagccgaagc
     9721 agtttccaca aaagaagaac gtgacaccgg ccacgatcaa ccatgtagtg gcttgcttct
     9781 tcagctcggc gttgggtccc ggtccgcccg gatagggccc aaatccgcca ggtcccgggg
     9841 gcggaccgaa cccgcccgga ggaggaccga agccccccgg cggctgcccg tagcctccgc
     9901 ctggaggctg accgaaccct ccaccgccgg ggccgtaacc gcccggtggc gggccgtagc
     9961 cgcccggtgg ttgaccataa cctccgccgc ccggaggagc tccgggagga ccaccacctg
    10021 gtggccctcc accgggagga ccgccgcccg gaggacccca acctccgggt ggataaccgc
    10081 ccggtggcgg tccataacca ccaccgccct gagcctcgat cactgaaccg ccaagtgtca
    10141 tttcttgatt ctatcctgca gtcgaaaacg aggcgcgctc gagttcaacc cgcctgctcg
    10201 gcgagtcgcc gcatgcttcg tccacggata gcccagtccc tctctagaat ctaccgcctc
    10261 gtgtgttcga gccgctcgcc cgcatcgggt ctcgataccc gtcatcccag tttcgaatcg
    10321 cttcgagcat ggagctcacg cagcctcgta ccgacttcca gctcgccaac gctaacccgg
    10381 ccaatcggcc cgcaaccttt cacgaatgac cgccaacgcg gcttcttcgc gcgccgcttc
    10441 gaggctctgt gaggagcaag cgcatgcgcg gcgagtccat gaaccacgaa cgcgagtgcc
    10501 gtgaagccga agatgcaacg caccagcgcg gaggtgactg cgctcgcgcg cggatgatgg
    10561 ctttcgtcgc ggtagtcggg atcgtggcgg gatgtcgagc tcgtcaagac gcggagcatc
    10621 gcgcacccga accggttcgt tcggcgagct atctacgggg ggaccgagtt ctggtcgaag
    10681 cgcgcgctgc agagttcttc gaggcgcgcg tgctcacgca acaaggtgat cgcttgcgcc
    10741 tgctacgtgc gagcggcgag gatgatctct gggtgtctcg agcggacgtc tatcgcctgc
    10801 ctccggagtc gaagggccac gccggaccgt gggcgatttg ctcgaacgag attgagccag
    10861 gctccgggcc cagctggatt ggctgtcgta tcacgaagga gtccggtgac cgactcaatg
    10921 tagagatgct ggatgggcgg agcttgacgc tcgcgaaccg ctccgtgttg cgcgcgtctc
    10981 cgttgaccga aatgaacttg cggcgtgcgt tcgaacaagc tcgtgcgcgc cgtgatttct
    11041 tggatcgagc agcacacgca gggcggcccc acgtgcccac gggatttcgc gctctaccgc
    11101 ggatgcgagt tctagctctg cgtcgaggcg gttggtacct gggcacggtg cgggagctcc
    11161 acaaagacgg agtcgatgtg gccttcgagt cgaagaccca gtacgagcgt atcccggcag
    11221 cggatttggt tcccgagcct ccgatgtccg aagctccact gcgcggctcg ttcgcgttgg
    11281 tgcgcccgct ctcaccattc gatgcctggc cagtgttgcg agtcgcaggg gtttcggatc
    11341 gcgagtttcg agtcgtcaac gaatccggcg aggaacgcgt cgtcgctcca agagacatcg
    11401 ttccgctgtt tccgtgagcg gccgcggggc gcgcgcacgt ggagcgcacg gccgcggtgc
    11461 ggtcgacgta tcagcgcgat gggctcaaca tccgggtggc tacgagcgct tgcgtgctac
    11521 ccgcaatgac gcgcagctcg gcgcgggttt tgagtctgtt ttctgcgctg tccgctgata
    11581 gagcgcaaac acgtgcggtg cccacctggc tgccccggcc aagcgctact tgcgcgccgt
    11641 cgctgccgac caagcggatc tcggctccgc tcgcgccggc gcccaaggcc agcgtcacat
    11701 ccatacagcg accgaacggt accgtgagat ccaagttctc gatgcgcgag tcgctcatat
    11761 cgaaagtccg aacttgaccg atctcgctcg cgcggcgaag cacgccacgc gacaccatgc
    11821 tctccagcag tcgattggcg gccagaggag cattcatcag attggcgggc acgtccggtt
    11881 cgccgctcgc ccagagtgca aacgggccag cgcgaagccg cgcttctaga tccaaacgcg
    11941 cgctgcgcgg gtgggcgctg caagtaaaaa acgtgagctg cgaacttgcg gcttgctgtg
    12001 cctcgatttc gagcgcgtcg ttcagcagaa acgcctggag tccacgcagt gggcgagccc
    12061 cgatcaaatc cagccgcgta cagcctgcct tgaggtgcaa ggcagacgag ctaagccggc
    12121 ccacctcggc ttgaccgctg agcacccgct gcgcgttggc gtagccgtgt tctctggcaa
    12181 gctgctggcg tagccgagtc tgttcctcct ccaaggaacc cgagggatat acttcgaccc
    12241 gtgcgatctc cgagtcgatg ctcgactcac tgccaggctt ggactgcgat aaggtgagcc
    12301 agccggggcc gcggcccgag agcggtctca actcgaaggt cacctgcgcc ggctcggagg
    12361 cgcaaacgac gagggcgcgg ttgcgaccca tggcccgcgc ccgacccaaa atcgccccag
    12421 attcatccag tgcattgagt tcgagatgac cgacctcgtt gctgggcacc agcacggcgt
    12481 ccagacagcg ctcggcatcg atcgttgccg agacccgcgt cggaaggcgc gcgtcgagcg
    12541 gcacgctgga acgccgctgg tcttgccatt cgccgccaat ctcttggcgg tgctctgcca
    12601 gcgcggtctc cagatcgacg ggaaccccgg tgcgcttggg gccgtcgctg cgcacaccca
    12661 gcgctgcggc cacttgggtc gcgagcttgg gctcgacgat ctgcgcccca agcgcgacca
    12721 agccgtagcc ggccgcaata cgggccgcga tccaaattcg cgtcgggtga ggggggcaaa
    12781 cgagcagacc gggcaccgta tccggcccct catcgctgcc caacacacta ccgtcttcgc
    12841 cataggcgaa cagatccaaa tcctcgatcg acgggcccgc gcgggcaatc atcacggcgc
    12901 aagactcgct cggaacgctc aacaaagcag agactcggtc gccgacactc cccggttctg
    12961 tcgcgagcac gcggctctgc gccgataggg cgtggatgcg cgcggccaaa ctcggcgcgt
    13021 cttcgcggcg cgcagtcttg gtttcgtttg cgcatggcgc gggcttcgcc gtccgacagg
    13081 cacaaaccag cagcccggcc gcgacgagca cacgtgcgat gttcgcgagc tcaccgctca
    13141 tggtgcgagc tccaaagaag acccgagggg ccgacggctg gtttgccaaa gcgaatacat
    13201 ccacacgacg ccgccgcccc gcgcgtcggt ctccacgcgc atgctccgct gttcccccgc
    13261 gcagaaagca atcaacgccc ccagctcgtc tcggctgcgg ttgcgcgtcc aagcgcgtcc
    13321 gacctgcgcc gcgatcgcca aagtctgagc gttacccgcc aggcgcacca gccccgccac
    13381 gtaacacgca ccgggctcga tctgcgccgg cattgcggtc ggccccgaca cacccatcgc
    13441 cacatcgatc ggatcgctcc ccacacgagt agcatgacgt cgcaagagcg cagcccacgc
    13501 cgcagttcca acggctccca gctggtctgg caaatgcgcg ggcaactcag agcgcgcgtg
    13561 agtcacccac acctctgctt gacgaggcgc aaacgaaacg ttgaacgtcg cgcgccccgc
    13621 ctcgccggcg caaaatccga aatgactcag ggcgccttca ccccgctgcc gctcgtagat
    13681 cgcctgacct gggctgagtt ccggctcgac cgagagctga ctcgactcac cccgggcgag
    13741 cgcttcggat gtcgagcgcc cctcggtcga accaacgggg tgcgcaggca agtcgagcaa
    13801 gaaatcgaat tcgtgacagc ccggctccac gcgctcggcg tagctgccgc cgaacgcgcc
    13861 gaggcgagtc agcttatgcg gtgcctccca gcgcaaacct tcgcgagccg cgcgctgctc
    13921 acgcgtcccc aagcgttgcg cgatgggcgc cagcgccgtc gtgttcagca ggggcgcggg
    13981 ttcgagcggg cccggatcgc ggtgaggcaa actatcggtt gcaggcggca ccggtgtggc
    14041 ggactccacg acgattgtct ccagcacggc ccggggcgac cgcagttcca cgactagcgc
    14101 ggcgagtttg gtttttcgcg cgccgcagta aaccagctcg gccaggcccg ctagactact
    14161 gactacaccg cgatcgggta agtccggtag ctcgggcata ctcgtggctc cgaccgcgcg
    14221 cagcgtaaag tgtgccgtca tcggccccaa taccgccacc gacacacaac tcgacggtag
    14281 tcccgaaaac acggcttcgg gcaggaagat cacgagcgga ctggatcgct caccgagccg
    14341 ggggggtagc cgaaacacgt gattcggagc gcgcccccgc gccgcgagca gctccgcgtc
    14401 cgcattgaga tcagcgctgg cgggctgcgc gaacagcagc ccgaacagca cagcaccact
    14461 ggcgtcgaca atcaggcgca aggcgcgccc aaggctcacg cctcctcgac tcgtgtgaag
    14521 ggtgcttcca cgaacgagcg tgaagcggga tcccatacga acagtcgatg ttgggtcacc
    14581 tcacccttat cgacggcggt gaccagatag gagagggcgt agctcggctc gcccgtgcca
    14641 cccagggtca tactcgcagc atcctcggca ctgaaatacg agccgcagtc taggtgcgaa
    14701 tgaaagaaca ctttcaccgg gcggccgcgg gccgcgccct cgctcaaggc ccgctcgaac
    14761 ttcaacgagt tgattttgaa gtactcgcgc cccgtgcgcg ggtgcccctc cggatcgacc
    14821 ttgtggtact tgttggccag attttcgagc tcaatcgctc gatcaactcc cagaggatcg
    14881 tgggcaggcc cctccagata gccgcacgct tcttcgtctc tcacgtaagc ttcgcgccct
    14941 gctcgttcca cttcggccag cacggaggcc ggaattcgaa tgcctccgtg aatccaaggt
    15001 tgatccattt atcgagtctc ctcggctcgc actcgcgagc cgttcttttg cgcgtcatgc
    15061 gttgacgggt tcttgatacc taaggctgct cctgctgcac ctttacgcgc ggagcaggtt
    15121 ttagcatcag accttggagc ctttcgcgca aagtcggctg ctgcgattcc agaatccgct
    15181 tgaaccgaat gccggctcgg cctaattccc cgagggcagg ttactacgtc ggtgccgtgt
    15241 tgagctgcgt cgcgctttcg ccgcacgcag cctcgcagag gtctctttat gctgcatttt
    15301 tctgtgatcc gattgctggc gtacttgctg ctcgccgtct tcgtcactcc ggcttacgca
    15361 gctccgcgga aaaagcccgt gaagcccgag gcggtcaacc tggatgagga ggaggcccag
    15421 gaagccccga agaaacaaga ggctccgaag aaagaaacgc cgaaacctgc cgcccccaag
    15481 gccacgccca aaccgccccc caaagtcgaa accgtcgatg tggaagaaga ggacggagag
    15541 ggcgagggta gcggtgatga ggaagaagat gcggggcaac aaactgcggg cctcgagtgc
    15601 acccccgccg tcagcgaaat tgaaacccgg cggccgattc ccatctcttg tgctccgacc
    15661 aagaaaggca tcgagcgcgt cgaggtgcgc tatcgagcgc ccgcgcgcaa gaagtggacg
    15721 aagctctacc tgcgcaagt
//
LOCUS       DASTKU010001052         3393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_876376, whole genome shotgun sequence.
ACCESSION   DASTKU010001052 DASTKU010000000
VERSION     DASTKU010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876376"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            572..1177
                     /locus_tag="VFQ61_37440"
     CDS             572..1177
                     /locus_tag="VFQ61_37440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960253.1"
                     /translation="MHGDGVWQLTSFSPTAVFVNDSPLAGSIQLEDEDELEILDNYFV
                     FRERDTEDSTYYELIYQLTIQDVPSRLANERIVREALTREAAHASRYRSSLAVASIGL
                     YCEDCRPTALDHYLRDFGEVLRQALGRAVTVGRIDSRELAIVAPNFDAGTLHDNISAA
                     HSIVQTNALTFPPCRIQIEVSAYHEGLTAADLLRKAARSAE"
     gene            complement(1231..1728)
                     /locus_tag="VFQ61_37445"
     CDS             complement(1231..1728)
                     /locus_tag="VFQ61_37445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960254.1"
                     /translation="MRSFGYVGPAEIRARAVEGNGGTPADSQEAVLTWLRDNFTPDAG
                     WATFVVDLSGTLLVAPRRTEHVACAAGNRVLAAGELRFARDGAVAEITNNSTGYCPAE
                     DCWPAVDAALKRAGLVGPSSFTFVARFRRCPRCLERNLIKDDWYYCAFCDSALPLAWN
                     FDERI"
     gene            complement(2032..2259)
                     /locus_tag="VFQ61_37450"
     CDS             complement(2032..2259)
                     /locus_tag="VFQ61_37450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960255.1"
                     /translation="MHPLIRSGALCTVAPVAPSALTVGDIVLCKVRANEYLHLVKAIQ
                     GERYQIGNNRGLINGWINGSAIYGRLVSVED"
     gene            complement(2414..2971)
                     /locus_tag="VFQ61_37455"
     CDS             complement(2414..2971)
                     /locus_tag="VFQ61_37455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HET9960256.1"
                     /translation="MANVDRTKTITRGTPESAAMVASIQRAAAITAKINRLTFNEADE
                     VRALFAELIGKKLDDRFFLNPPFYTTGGADIRVGRNVFINQNCTFYDLGGLDIADDVM
                     IGPNVSLMTSGHPLEPSRRHGSVVAKPITIQKNVWIAAGVTVIGGVTIGENAVVAAGS
                     VVTKDVPPNTLVGGNPARFIRSIHE"
     gene            complement(2976..>3393)
                     /locus_tag="VFQ61_37460"
     CDS             complement(2976..>3393)
                     /locus_tag="VFQ61_37460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HET9960257.1"
                     /translation="FTRHQRRASALRSHSGTSERPALSSHAMTAAAEPTDPTPTVAVL
                     GTLRFPPDRVPEVLPHLQRLVANTYEKDGCIAYDVAIDPFDPGLIRFSELWPDQASLE
                     RHLVAPHIAPWRAAARACGLMARSFTAFEVSSARPV"
BASE COUNT          666 a         1044 c         1055 g          628 t
ORIGIN      
        1 gtgcagaccg tcaactgcat cacgaacaac atcggtttga gccaccggta cggcgcgtcg
       61 gtcgacgtcg ctttacgacc ctgcgcgtag agacgattct tccggattcg tgagtgcaac
      121 gtcccccacc aaaccgccca ccccaggagg agactcgtga agagaatgga gttcgccata
      181 gccgacgccg acacgttagc tcagcgcatg gctgcggccc accatcgagt cggtcaaggc
      241 gtagcctggg tggacggctc tgcttggcgc ggcccgcacg gttggcgctt gtcgcgaagc
      301 tgggatgtcc tagccttgct tgatgcagcc accgcgctcc acagttgccg tcgtcgacgc
      361 aaccgctgcg ggcaactccg gcggcgctga ggggtcaggg cgggcaaacc ggaagccgcc
      421 gcaggagccc gcgtgtctgg tgctcatgca ccggccctct cggctcggca tcggtcgcag
      481 gcaccccgtc ttcgctcacg ggtttagact cgggaactcc gctgatgatt tcgccctctc
      541 agcggacgca ggtcttgctg cggagatcgc aatgcacggg gacggcgtat ggcagctcac
      601 gtctttctcc ccgaccgcag tgttcgtcaa tgactcgccg ctcgcgggaa gcatccaact
      661 cgaggacgag gatgagctcg agatcctgga caattacttc gtttttcgag agcgggacac
      721 cgaagactcg acctattacg agctcattta ccagctcacg atccaagacg ttccgtcccg
      781 cttggccaat gagcgaatcg tgcgcgaagc cctcacccgg gaagccgctc acgcttcgcg
      841 ctatcgaagt agccttgcgg tcgcgtccat cggcttatac tgcgaagatt gccggcccac
      901 ggcgctcgac cactaccttc gagacttcgg cgaagtgttg cggcaggcac ttggtcgtgc
      961 tgtgacagtg ggcagaatcg atagtcgtga gttggctatc gtcgcgccca acttcgacgc
     1021 gggcacgctc cacgacaaca tatctgccgc gcactccata gtgcaaacga acgccctcac
     1081 cttccctcca tgtcgaatcc agattgaagt ctccgcgtac cacgaaggcc ttacggccgc
     1141 tgaccttctc cggaaggcgg cgcgcagcgc ggagtgaccg aaaacggcag caagaaagcc
     1201 gaattgttgc tctgcactgc ggccgtactc tcagatccgc tcatcgaagt tccaagcgag
     1261 cggcagggcg gagtcgcaga atgcacaata gtaccaatca tccttgatca agttccgctc
     1321 gaggcagcgc gggcagcgcc tgaatcgcgc gacgaaggtg aacgagcttg gccccacgag
     1381 gccggcacgc ttcaacgcgg cgtcgactgc gggccagcag tcttcagccg ggcagtaccc
     1441 cgtcgagtta ttggtgatct ccgcgacggc accatcccgc gcgaagcgaa gttccccggc
     1501 ggcaaggacg cggttccccg ccgcgcaagc tacatgctcg gtccttcgcg gagcaacgag
     1561 aagagtgcca gatagatcga ctacgaatgt tgcccaaccg gcgtcgggag tgaagttgtc
     1621 gcgcaaccac gtgagcactg cctcctgaga atcagccggt gtccccccgt tgccttcaac
     1681 tgctcgcgcc cgaatctccg cgggacccac ataaccaaag cttcgcattg cgttcccaat
     1741 tccgattgag cgagagaccg ttaccgtcta ggctcgcgcg tggccgctgc cgcacactgc
     1801 actgtgcaat ggcagtcgac ggggcgcaag gcgcatgggt atggcgcacg ggtatggccc
     1861 atgactccga cgagccccgc ggttgcgacc ggtccgcccg aatacagggt ggggcgtgcg
     1921 cggtggattg actggagagg ggcggcggag tctgcgcgga tgagggtagc gacgctcagt
     1981 cgcgcccgtt gtttcgccgg gcccgcaccc gaggaggtaa acgcctggca attagtcttc
     2041 aaccgagacg agacgtccgt agatggcgga gccattgatc caaccgttga ttaggcctcg
     2101 gttattgcca atctggtaac gctcaccctg gatcgctttg acgaggtgga gatactcgtt
     2161 tgctcggacc ttacagagca cgatgtcccc gaccgtcagg gcgctcggcg ccacgggcgc
     2221 gacggtgcag agagcgcccg agcggatgag cggatgcatg gagttccccc tcggacgaaa
     2281 ggatactgtt tcgcccgcct tgagtttcgc gatgtgacct gttgcccaac ccacgggaga
     2341 ggttctaaca cacaaagtgt ctcccgaaaa cccgaaccat gttgggttat gctcaccccg
     2401 acgtgcttac ccttcattcg tgaatcgagc ggatgaatcg cgccgggttc ccgccgacga
     2461 gcgtgttcgg gggaacgtcc ttcgtaacta cggatcccgc ggcgacgacg gcgttctcac
     2521 ctatcgtcac ccctccgatg acggtaacgc cagccgcaat ccacacgttc ttctggatcg
     2581 tgatgggctt ggcgacgacg ctgccatgac gccgtgacgg ctcgagaggg tggccggaag
     2641 tcatcagact cacgttggga ccaatcatga cgtcgtcggc aatatcgagc cccccgagat
     2701 cgtaaaacgt gcagttctgg ttgatgaaca cattgcgccc aacacggata tcggcgccgc
     2761 cggtcgtgta aaacggtggg ttcaagaaga accgatcatc cagcttcttg ccgatgagct
     2821 ccgcaaacaa ggctcgaacc tcatctgcct cgttgaacgt caaacggttg attttggccg
     2881 tgatcgcggc ggcgcgttgg atactcgcaa ccatggccgc ggactccggg gttccccggg
     2941 taatcgtctt ggttcggtcg acgttggcca tgcgactaga ccggacgcgc cgaagagact
     3001 tcgaatgcgg taaagctacg agccatgagc ccgcaggcac gagctgcggc gcgccagggc
     3061 gcgatgtgag gcgcaacgag atgccgttcc aaagaggctt ggtcgggcca aagttcagag
     3121 aagcggatca agcctgggtc gaaggggtcg attgccacat cgtaagcaat gcatccgtct
     3181 ttttcgtaag tattcgcgac caggcgctgt aaatgaggca gcacctcagg gacgcgatct
     3241 ggcggaaagc ggagcgttcc cagaacagca acggtgggcg ttgggtctgt cggttcggcg
     3301 gcggcggtca tggcgtgaga acttagcgcg ggccgctcgc tggtaccaga gtgcgagcgg
     3361 agtgcgctcg cccgtcgttg atgacgggtg aag
//
LOCUS       DASTKU010001053         2076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_653306, whole genome shotgun sequence.
ACCESSION   DASTKU010001053 DASTKU010000000
VERSION     DASTKU010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653306"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VFQ61_37465"
     CDS             <1..718
                     /locus_tag="VFQ61_37465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960258.1"
                     /translation="SSPVLYRVVTLAGRDLGSLEQEFVDRLVDQMSSFLLGGRAWTVE
                     RVSHEDRAVVVRDAPAGMKPSWGGFVPQHLGFELCQRIMRVLTEDTKYPYVDEAGLKH
                     IEAKREDLGALLKRPGRAIQLDGAVARWWTFAGGRVNHTLKYGFEIADGWKVVADNFQ
                     LRIEGDGVNHETVRAAIAKMASAGFWAAPGTRKAVLARLPNYRLSKFQDCLPEEYALE
                     VVERYLLDVEGTVAWLASTN"
     gene            1114..1614
                     /locus_tag="VFQ61_37470"
     CDS             1114..1614
                     /locus_tag="VFQ61_37470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960259.1"
                     /translation="MKRVPPSVVQAADPRESASGQDDALAGQRESCERLAVWPRAPDL
                     DDADDHAGPAGGAAPDVSARVHKQFRPGSVEALAAALEQLSPELERSGEQLADALRQV
                     ARSFELLPTTCVPDDGSEASEAHHVGLAFAHYTLQRLSCQYSVARKDVLALREKVRAL
                     RDESLA"
     gene            complement(1624..>2076)
                     /locus_tag="VFQ61_37475"
     CDS             complement(1624..>2076)
                     /locus_tag="VFQ61_37475"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9960260.1"
                     /translation="GEVVVSPSPSFFHQEVVGRLSVLLRTWAAGQPNAVTVGQAPTDI
                     RFAKGRILQPDLFVIFERIPLSHEGPLDRVPELCIEVLSSRPNYDRLAKRSVYADAGV
                     QELWTVEPGQYVERWTGDGLRRMAKLESVVESSLLPGLTIALDTLFRE"
BASE COUNT          367 a          629 c          717 g          363 t
ORIGIN      
        1 ctcgtcgccg gtgctctaca gggttgtgac cctggcgggt cgagacctcg gttcactgga
       61 gcaagagttc gtagaccgct tggtcgatca gatgtcgtcc ttcttgcttg gtggccgcgc
      121 ttggacggtc gagcgggttt cgcatgagga ccgtgcagtc gtggtgcgcg acgccccagc
      181 cggcatgaag ccaagctggg gcggtttcgt cccgcagcac ctcggctttg agctgtgcca
      241 gcgcatcatg cgtgtgctca ccgaggacac gaagtatccg tacgtcgatg aggcagggct
      301 caagcacatc gaggccaagc gtgaggactt gggcgcgctg ctcaagcgac cggggcgtgc
      361 cattcagctc gatggtgccg ttgctcgctg gtggacgttc gcgggcgggc gggtgaacca
      421 cacgcttaag tacggctttg agatcgccga cgggtggaag gtggtcgcgg acaacttcca
      481 gcttcgcatc gagggcgatg gcgtgaacca cgagaccgtg cgcgcggcca tcgcgaagat
      541 ggcttccgcg gggttctggg ctgcaccggg aacgcggaag gcggtgctgg cgcggttgcc
      601 caactatagg ctttcgaaat tccaggattg cttgccggag gagtacgcgc tggaggttgt
      661 ggagcgatac ctgctcgacg tcgagggcac ggtcgcgtgg ctcgcgagca cgaattagac
      721 gacgaggccc gctgtgggag taggcgagta cccaagcgaa agccaatgag tgttcagctg
      781 gccttgggct cgctttttcg tggccaagcg gaccttcaaa ttgtaccaga tgcccgctcg
      841 ctggcggttc gggagctgct ggtacaatca ccggtacgat atgcatgcat cgcgggccaa
      901 catcgttctc gccgggtcac ctcaaatgtg cagaaatact gcaaagacga ggaatggaga
      961 ggcaccgaga cgagcgcgtc tgtcggtcta cggaaccgaa agtcggaggt tcgaatcctt
     1021 ccgggcgcgc aactcaaggc atgacgtaga gctcgctgta ttcggtgcgc gcgcgcactg
     1081 cccaaaaaac gcgcggcacg ttatgacggc cccatgaagc gagtgccacc gagcgtcgtg
     1141 caggccgcgg acccgcgcga gagcgcgtcg ggccaggatg acgcgctcgc cggtcaacgc
     1201 gagagttgtg agcgcctggc ggtctggccg cgcgctccgg accttgatga tgcagacgac
     1261 cacgcggggc cggcgggcgg cgcagcgccc gacgtaagcg cacgcgttca caagcagttc
     1321 aggccgggct cggtggaggc gctggccgcg gcgctggagc agctctcgcc ggagctcgag
     1381 cgctccggcg agcagctcgc tgacgcgttg cggcaagtcg cgcgtagctt cgagctcctg
     1441 cccaccacgt gcgtacccga cgacgggtcg gaggcctcgg aggcgcacca cgtcggcctc
     1501 gcgttcgcgc attacacgct ccagcggctc tcctgccaat acagcgtcgc gcgcaaagac
     1561 gtgctcgccc tacgcgaaaa ggtgcgcgca ttgcgcgacg agtccttggc gtagaggtta
     1621 cgtttactcc cggaacagcg tatcgagggc gatggtcaga cctggcagca ggctgctttc
     1681 gaccacgctc tccagcttgg ccatgcgccg taggccgtcg ccggtccagc gttcgacgta
     1741 ctgacccggc tccacggtcc agagttcttg aacgccggcg tccgcgtaca cgctgcgctt
     1801 cgcgagccga tcgtagtttg gccgggatga cagcacctcg atacatagct ctggcacccg
     1861 gtccagcggc ccttcgtggc tgagcgggat gcgttcgaag atcacgaaca ggtccggctg
     1921 caaaatgcgg cccttcgcga agcgaatgtc ggtcggggcc tggccgacag tgacggcgtt
     1981 aggttggccg gcagcccagg ttcgaagcag gaccgaaagt ctgcccacga cctcctggtg
     2041 aaagaagctg gggctaggcg acacgacgac ctctcc
//
LOCUS       DASTKU010001054         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1325561, whole genome shotgun sequence.
ACCESSION   DASTKU010001054 DASTKU010000000
VERSION     DASTKU010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325561"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1159)
                     /locus_tag="VFQ61_37480"
     CDS             complement(170..1159)
                     /locus_tag="VFQ61_37480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9960261.1"
                     /translation="MESSSDRDSVGRSEIDPPDETSSVLVTGGCGRLGRRLIRALHRD
                     TRVVSIDKRAAPGFPEDVEHHALDLLSKSVRDVFRHIRARAIVHLGITHDPRLGWEQH
                     HAWNLLAIQRVLEFAEQFGIPKVVLLSSANVYGPRPDNAQFLREEAPLLAAGRFTDIG
                     ALVEIDLATQSFLWRQPHINTVILRPVHILGSVRNAPSNYLRLKRVPTLLGFDPMMQV
                     VHQDDVVSSVQLALKPEVRGIFNLAGPAPVILSRAIQLLGRSALPLPHGAARYAVERM
                     FRWRMTSFPAAELDFIRYVCMADDTRARTILGYAPRHDLASTLAAVDAERWVD"
     gene            complement(1143..2066)
                     /locus_tag="VFQ61_37485"
     CDS             complement(1143..2066)
                     /locus_tag="VFQ61_37485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9960262.1"
                     /translation="MDPRKNALVRASDLAAQGGAFFSRLLQGAKRVADEVSERVLGAD
                     FDRRVALLSERYESAQDPFGFDPEIARKAAATCAFFHRLYFRTEVFGIENVPQGRALL
                     IANHSGQIPIDAAIIGSAMFLDAEPPRVTRSMVDKWTATLPFVSMFFSRVGQVVGLPE
                     NARRLLAMEELLLAFPEGTRGVAKPFSKRYELEAFGRGFMRLALETNTPIVPVAVIGA
                     EEQYISFGNLTWAAKALRLPVFPLVPQVLLPGGQLPLPTKYRIYFGEPMRFYGDPDDD
                     EQVIADKVWLVRQTIAHMLTQGLKARRGVFF"
     gene            2242..3171
                     /locus_tag="VFQ61_37490"
     CDS             2242..3171
                     /locus_tag="VFQ61_37490"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET9960263.1"
                     /translation="MTEFVMPRALQPGDEIVVIAPSSPFDRALFFRGVGWLAERYRVR
                     LGRHVLERRGFLAGSDEGRLADLNEALREPGVRAIVAARGGHGAVRLMSRADWAAFER
                     APKWIVGYSDITLLHAEAWSVGVCSLHAENAAGLGRGSAAARQRWLSAIEQPTQERCF
                     EGLESLHGGAARGPLVGGNLTLLFTAQACARLKIPLGAVLALEDVTEASYRVDRMLSA
                     LAIAGVFDSISGVVLGDFTDCSPGPHRVPVQSVLEEHFSRLGIPVLRGLSFGHGAHNE
                     PLPFGLEAAIEHDSLRIGLNGSASLDARRRAGA"
     gene            complement(3257..>3838)
                     /locus_tag="VFQ61_37495"
     CDS             complement(3257..>3838)
                     /locus_tag="VFQ61_37495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960264.1"
                     /translation="VAVDLAKQPEIRAELGALGLGALADGERAGMGMAMVEPAASEVA
                     ATVPEAAAASTEDESGLEQTVEPEPAPPPPPPSEPFAVKSSEVPRQGPPPKPSRRPAP
                     EQSVSVGAGGGTPSRPLPPPPRGRSAPPPPPGGSRPAPPPLPSARPGGARPPLPPPGL
                     RRPPSDTISQTARANEDEEFSVDDDELIEDGTA"
BASE COUNT          665 a         1243 c         1262 g          668 t
ORIGIN      
        1 gatgggcggc tgaaatgcac gaggctgtga agctgcgtag gcgcccccgg ccagcggcgc
       61 ggccgccacg aaatccaaat tgagcggcgg gggcgccgct tcgctctcgt gctccgcgca
      121 gccactcgcc gagacgaggc ctagccaaac caccatccga aggcgacgtt caatcgaccc
      181 agcgctctgc gtccaccgca gccagcgtcg aagcgagatc atgccgagga gcatagccca
      241 aaatcgtgcg ggctctggtg tcgtcggcca tacagacgta gcgaatgaag tccagctctg
      301 ctgcggggaa agacgtcatt cgccagcgga acatgcgctc cacggcgtag cgggcggccc
      361 catgcggcag tggtagcgcg ctgcgcccca acagttgaat cgcccgcgag aggatcaccg
      421 gagccgggcc ggccaggttg aagatgcccc gaacttcggg cttcagcgcc aattggaccg
      481 agctcacgac gtcgtcttgg tgcacgacct gcatcatggg atcgaagccg agcaacgtgg
      541 gtacgcgttt gagccgcaga tagttgctcg gcgcgttgcg gacgctgccc aggatgtgca
      601 ccggacgcag gatgaccgtg ttgatgtgcg gctggcgcca gagaaagctt tgggtcgcca
      661 agtcaatctc gaccagcgcg ccgatgtcgg taaatcgtcc ggctgccaac agcggcgcct
      721 cctctctcag aaactgagcg ttgtccgggc gcgggccgta aacattggcg ctcgagagga
      781 gcacgacctt ggggatcccg aactgctccg caaactccaa tacccgctgg atcgcgagca
      841 gattccaggc gtgatgttgt tcccagccga gccgcggatc atgcgtgatc cccaggtgaa
      901 cgatggctcg cgcgcggatg tgccggaaca cgtcgcgcac actcttgctc agcaaatcca
      961 gagcgtggtg ctcgacatcc tcgggaaacc ccggggctgc tcgcttgtcg atcgagacca
     1021 cccgggtgtc ccggtgcaga gcccggatca gccgacgtcc aagccgcccg cacccgccgg
     1081 tgacgagcac cgagctggtc tcgtcgggag ggtcgatctc cgacctgccg accgagtccc
     1141 gatcagaaga agactccacg gcgtgctttc aagccctgcg tcagcatatg ggcgatggtt
     1201 tgccggacga gccagacctt gtctgcgatc acttgctcat catcatcggg atcaccgtag
     1261 aaccgcattg gttcaccgaa gtagatgcgg tacttggtcg gcagaggtag ctgccctccc
     1321 ggcaacagaa cctgaggaac gagcggaaat acgggcaagc gcagagcctt tgcggcccag
     1381 gtcaagttgc cgaagctgat gtattgctct tccgcaccaa tcacggcaac cgggacgatg
     1441 ggagtgttgg tctccagcgc caaccgcatg aagccccggc cgaacgcctc cagctcgtaa
     1501 cgtttcgaga acggcttcgc caccccgcgc gtgccctccg gaaaagccag gagcaactct
     1561 tccatggcca gcaagcgacg ggcgttctcg ggcagcccca ccacttgccc gacgcggctg
     1621 aagaacatcg agacgaacgg caaggtcgcc gtccatttgt ccaccatcga gcgcgtcaca
     1681 cgcggcggct cggcatccaa gaacatggcc gaaccgatga tcgccgcatc gattgggatt
     1741 tggccggagt gattggcgat gagcagcgcg cgaccttggg ggacgttttc gatcccgaac
     1801 acctccgtac gaaagtacag gcggtgaaag aacgcgcacg tagcggcagc ctttcgcgca
     1861 atctcaggat cgaatccgaa tggatcttgt gccgattcgt aacgctccga gaggagcgcc
     1921 acacggcgat cgaagtccgc gccgagcacg cgctcggaga cctcgtccgc gactctcttt
     1981 gcaccctgaa ggaggcgcga aaagaaggct ccgccttgag cggccaagtc gctggcgcga
     2041 acgagagcgt tttttcgagg atccatccag aaaccccggg cacgttacac gatagtccga
     2101 gctccgcgag ctcttcgtac gttgcgatgt ccggtcgctt gtccagaccg gccacaagct
     2161 tgacacgtgg cgcttgaggc gccttaaagc ggctgtgact agccaccccg atatcgtcat
     2221 ccgcgagggc agccgccgac catgaccgag ttcgtgatgc cgcgcgcttt gcagccgggc
     2281 gacgaaatcg tcgtcatcgc tccatcgagt ccgttcgacc gagctttgtt ttttcgcggc
     2341 gtgggctggc tcgcggagcg ttaccgggtg cgtttgggcc gccatgttct cgagcgcaga
     2401 gggtttctgg cgggctcgga cgaaggacgc ctggccgact tgaatgaagc cctgcgcgag
     2461 ccgggggtac gggcgatcgt cgcggcccgt ggcggtcatg gagcggtccg tctgatgagt
     2521 cgagccgatt gggcagcgtt cgagcgcgct ccgaagtgga tcgtcggtta ctcggatatt
     2581 accctcctgc acgcggaggc ttggagcgtc ggcgtttgca gtttgcacgc cgagaacgcg
     2641 gccgggcttg gtcgcggctc agccgcggca cgccagcgct ggctctccgc gatcgagcaa
     2701 cccacgcagg agcgttgctt cgaaggcttg gagagtctcc acgggggtgc ggcgcggggc
     2761 ccgctcgttg gcggcaatct gacgttgctg tttaccgcgc aagcctgcgc cagattgaag
     2821 attccgcttg gcgccgtgct cgcgttggaa gacgtgacgg aggcttccta ccgcgtggac
     2881 cgcatgttga gcgcacttgc gatcgcgggt gtcttcgact cgattagcgg cgtggtgctc
     2941 ggagatttca cggactgctc gccagggccc caccgcgttc ccgtgcagtc ggtgctggag
     3001 gaacactttt cgcgcctcgg gatcccagtg ctacgaggcc tctcatttgg ccacggcgcc
     3061 cacaacgaac ccctcccgtt cggcctcgaa gcggccatcg aacacgactc tcttcgaatc
     3121 ggcctcaacg ggtcagcttc gctcgatgcc cggcgccgag caggcgcgtg attgccgcac
     3181 tcagttgcgg cgcactggcc gagcccgccc gactcaaccg ttgggattgc gattgagtcg
     3241 gggctccggc gtccgctcac gcggtgccgt cttcgatgag ctcgtcgtcg tcgaccgaga
     3301 actcttcgtc ctcgttcgcg cgggccgttt gagagatggt gtcgctcggt ggtcgccgta
     3361 gcccaggagg cggcagcggc ggccgagcac caccgggccg cgcgctgggc agcggtggcg
     3421 gtgcggggcg cgaaccgcca ggcggtggcg gcggggcgct gcgaccacgt ggcggcggag
     3481 gcaacggccg actcggtgtc cccccccccg cgccaacgct caccgattgc tcgggcgccg
     3541 gacgacgtga cggcttgggc ggcgggccct gacgcggaac ctcgctcgat ttcaccgcga
     3601 atggctccga tggcgggggt ggcggcggag ccggttctgg ctctaccgtt tgctcgagcc
     3661 cagactcatc ctcggtgctc gcagctgctg cttcgggaac cgttgccgcg acttcggatg
     3721 cggcgggctc caccattgcc atgcccatgc ctgcccgctc cccatcagcg agggcgccca
     3781 acccgagcgc tccgagttcg gcgcggatct ccggttgctt tgcgagatcg accgccac
//
LOCUS       DASTKU010001055         6735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1996796, whole genome shotgun sequence.
ACCESSION   DASTKU010001055 DASTKU010000000
VERSION     DASTKU010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6735
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996796"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..495)
                     /locus_tag="VFQ61_37500"
     CDS             complement(73..495)
                     /locus_tag="VFQ61_37500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960265.1"
                     /translation="MRSARIFAVLNLLPAYVLGAGCYILPSRFWPLDSAVIALVCVLV
                     ATSVVAFARPALAHRALRFAALTLLVIGLGVCAMFALTAAFLYSVHGSYGDLGAILMS
                     LVLLLIVPYSLAYPSLLLLWLAKNRAPSPVAQLASGPQ"
     gene            complement(497..4393)
                     /gene="purL"
                     /locus_tag="VFQ61_37505"
                     /gene_synonym="purI"
     CDS             complement(497..4393)
                     /gene="purL"
                     /locus_tag="VFQ61_37505"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET9960266.1"
                     /translation="MLVLRGAAALTPFRRARKLEELKKLQPSLTGLSAEHWYFVETQS
                     AIEPFKLDRLLSIVEATPLQASSAEAAQRAAHLIVVPRLGTISPWSSKATDILHVCGL
                     PEVGRIERGTAYRFEGAVSIDWVSSFMTLLHDRMTETVLRSPDEARSLFRHEAARSVR
                     HVDVLGQGRPALERANAELGLALAEDEIDYLVTSFQELGRNPSDVELMMFAQANSEHC
                     RHKIFKADFIIDGVKQPHSLFKMIQNTHQLRPEGTLSAYRDNAAVFEGSKGLRLFPDP
                     HSGVYGEVAEDVHILIKCETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKVGLT
                     GFSVSNLHIPGFEQPWETNPVGKPDRIASALDIMIEGPIGGAAFNNEFGRPAILGYFR
                     TFELPDGDVVRGYHKPIMLAGGLGNVRPMHVQKRPFAPGACLVVLGGPAMLIGLGGGA
                     ASSVASGASSADLDFASVQRENAEMQRRCQEVIDRCNALGEASPIAFIHDVGAGGLSN
                     ALPELVHDSERGGQFELREIPSAETGLSPLELWCNEAQERYVLAISPERLETFRAIAE
                     RERCPFAVVGHATQEPQLVLSDRDQGDKPIDIPMSLLFGKPPTMTRDVQRVPRRRVEF
                     ATQQIDVADAAARVLRLPGVASKNFLITIGDRTVTGLVARDPLVGPHQVPVADAAMSL
                     TDYRGYCGEAMSIGERAPIALLNPAASARMAVGEAITNLAATPVESLSDVRLSANWMA
                     AAGHPGEDAALYEAVRALGMELCPTLGISIPVGKDSMSMRTVWENGTKRVVAPVSLVV
                     SAFARVSDVRRGVTPELCRTREPGCLIHIDLSLGKHRLGGSALAQVYGQLGSQAPDLD
                     DPNLLTTFFAATRELLEQHLMLAYHDISDGGLFVTLCEMAFAGGTGLEIELESVGHDV
                     IAALFAEELGAVLEVPRDRVDTVFAILERHGLRRGRGQACHLLGTPSSQDAIVIKQRG
                     KVVFSAPRSQLRRIWSEPSYRIQALRDNAHTAKQEFELASDPHDPGLSMRLTFDPEER
                     AEDVGRIQSAERPRIAVLREQGVNGQIEMAAAFTRVGFAAVDVHMTDILSGRLTLESF
                     KGLAACGGFSYGDVLGAGQGWAKSILFSPRAREAFEHFFRRTDTFGLGVCNGCQMFSG
                     LKGLIPGADAWPRFLRNQSEQFEARLSMVEVQSSKSLLFAGMSGSRMPVVVSHGEGRA
                     EFSSEEQRRICEREQLVALRFVDHYGRTTEHYPENPNGSPDGITSLTTPDGRFTALMP
                     HPERAFRTVQLSWHPEHLGERSPWLRMFANARRWVG"
     gene            4864..5649
                     /locus_tag="VFQ61_37510"
     CDS             4864..5649
                     /locus_tag="VFQ61_37510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960267.1"
                     /translation="MSGCVLSDPFFIDPSASEGGASSQGGTGGASETARGGTTPKGGN
                     TSGGTSPVGPKAGGPGLGGSPLGGTPSTTSVQGGFTGTTGHQGGSGGASNTQAGGAGG
                     TVQSSTIQASGGSAGNWILADFETSTEGFGPPPEKQNSNTVSTTAFYHTQGKQGLLAT
                     MRSAEWVGAMFPQALDLSRANRIKVDIRTSTAPLQADVAIVTGSDSWCSGNIMIQVPA
                     QTATTLEVNLKTRTCANFDASRVRGVYIWFEVGTYYADYMRTE"
     gene            5657..>6735
                     /locus_tag="VFQ61_37515"
     CDS             5657..>6735
                     /locus_tag="VFQ61_37515"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9960268.1"
                     /translation="MTETVDSDKALRLRSEPAAPAAEPSSYLQGEVIGDRYRLERELG
                     RGGMGVVWVAHSLVLGIDVAVKLIRAETAGPAVASRMAREAHAAARLGHPALVRVFDF
                     GWTGRGDPFLVMELVQGETLSEMIRRECQVPVIRAVQILLPIADGLRIAHDRSIVHRD
                     IKPDNILLSTDSLGRLQPKLLDFGIAKVGPTVGEGKLTQFGTVLGSPEYMSPEQALGL
                     DDIDERTDVWSFCSALYEMVTGSIPFAKSNYNALMHAIISEEPQSTVSQGVGDAAFWA
                     ILERGLTKERDKRFRNMTELGEALALWLYEHGIKADSCGNSLRAVWLDVSMGTLLPAV
                     ELSIPPGSVIANSSSLSPAIAARTTR"
BASE COUNT         1281 a         2205 c         2121 g         1128 t
ORIGIN      
        1 accacaggcc cacgcacgcg acgcccaacg tcgcgaggta caccttcgcc catttacgga
       61 aatcgagctc tctcactgcg ggccggacgc caactgcgcg accggcgagg gtgcgcggtt
      121 cttcgcgagc caaagcagaa gcaggctcgg atacgccaat gagtagggca cgatcagcag
      181 caggaccagg ctcatcaaga tcgcgcccaa gtccccgtag gacccatgaa ccgaatacaa
      241 gaacgcagcg gtcagcgcga acatcgcaca gacgcccagg ccaatcacga gcaaggtgag
      301 cgcggcgaaa cgcagcgccc ggtgcgccaa cgctggtcga gcgaaggcga ccacactcgt
      361 ggccacgagc acgcaaacga gcgcgatgac cgcgctgtcc aatggccaga agcgcgacgg
      421 caggatgtag cagcccgccc ctaatacgta ggccggcaac aagttcaaga ccgcgaagat
      481 ccgcgccgat cgcatctcag cccacccaac gtcgggcgtt cgcgaacatc ctcagccagg
      541 gggaccgttc accgaggtgc tccgggtgcc acgagagctg cacggtccga aacgcgcgct
      601 cggggtgcgg catcaatgcc gtgaagcgac catcgggggt cgtgagcgac gtgataccgt
      661 cgggcgagcc attcggattt tcggggtaat gttcggtagt gcgaccgtag tggtcgacga
      721 accgcagcgc caccaactgc tcgcgctcgc agatccgtcg ctgctcctcg ctcgagaact
      781 cggcgcggcc ctccccgtgc gagaccacga ctggcatgcg tgacccggac ataccggcga
      841 acagaagcga cttggagctc tggacttcga ccatgctcag acgcgcttcg aactgttccg
      901 actgatttcg caggaagcgc ggccatgcgt cggcacctgg aatcagccct ttgagtccgg
      961 agaacatctg gcagccattg cagacgccca aaccgaacgt gtccgtgcgg cggaaaaaat
     1021 gctcgaacgc ctcacgggcg cgcgggctga acaagatcga cttggcccag ccctggccgg
     1081 caccgagcac gtccccgtag gagaagccgc cgcaagccgc cagccccttg aagctctcga
     1141 gcgtgagcct gccgctcaag atgtccgtca tgtgcacgtc gacggcggcg aagccaaccc
     1201 gcgtgaacgc cgccgccatc tcgatttggc cgtttacgcc ctgctctcgc agcaccgcga
     1261 tacgcgggcg ctccgcgctc tggattcgcc cgacgtcttc ggctcgctct tcgggatcga
     1321 acgtgagtcg catcgagagc cccgggtcat gcggatcact ggccagttcg aactcctgct
     1381 tggcggtgtg tgcgttgtca cgcagggctt ggatccggta actgggctcg ctccagatgc
     1441 ggcgcagctg actgcgcgga gcggagaaca cgaccttgcc gcgctgtttt atgacgatgg
     1501 cgtcctgtga gctcggcgtt ccgaggaggt ggcaggcttg gccgcgtcca cgacgcaaac
     1561 cgtgacgctc caggattgca aacaccgtat ccacccgatc tcgaggcacc tcgagaacgg
     1621 cgccgagctc ctccgcgaac aaggccgcaa tcacgtcgtg ccccacactc tccagctcga
     1681 tctcgaggcc cgtcccgccc gcgaacgcca tctcgcacaa cgtgacgaac aagccaccat
     1741 cggagatatc gtgatacgcg agcatcaaat gttgttcgag taactcccga gtcgcggcga
     1801 agaacgtcgt gagcagattg ggatcatcga gatcgggcgc ctgggagccc agctgaccgt
     1861 agacctgcgc caaggccgac ccacccaggc gatgtttacc gagcgatagg tcgatgtgaa
     1921 tcaggcagcc cggctcacgc gttcggcaga gttcgggcgt gaccccgcgc cgcacgtccg
     1981 aaacccgagc gaacgccgac accaccagcg agacgggcgc caccacgcgc ttggtgccgt
     2041 tctcccagac cgtccgcata ctcatcgagt ctttgccgac ggggatagaa attccgagcg
     2101 tggggcacag ctccattccg agggcgcgca cggcttcata gagcgcggca tcctcacccg
     2161 ggtgccccgc tgcggccatc cagttcgccg agagccgcac gtcggacagg ctctcgaccg
     2221 gagtggccgc caggttggtg atcgcctctc cgacggccat ccgagcagat gcggccgggt
     2281 tgagcagcgc aatcggggcg cgctctccaa tcgacatggc ctcaccacag taaccgcgat
     2341 aatcagtgag cgacatggcc gcgtccgcga ccggcacctg atgaggcccc accagcgggt
     2401 cccgtgcgac caagccggtt acggtacgat ccccgatcgt gatcaggaag ttcttggagg
     2461 ctactccggg caggcgcaaa acgcgggccg ccgcgtcggc gacgtcgatt tgctgggtcg
     2521 cgaactcgac ccgacgacgg ggtacgcgtt gcacgtctcg cgtcatggtc ggcggtttgc
     2581 cgaacagcag cgacatcggg atatcgatcg gcttgtctcc ttggtcccga tccgagagca
     2641 ccaactgcgg ctcttgcgtc gcgtgtccca ccacggcgaa ggggcagcgc tctcgctccg
     2701 cgatcgcgcg gaaagtctcc agacgctccg gcgagatcgc gagcacgtaa cgttcctgcg
     2761 cctcgttgca ccacagctcg agcggcgaaa gtccggtttc cgcgctcgga atctcccgca
     2821 gctcgaactg tccgccacgc tccgagtcgt gcacgagctc gggcagtgca ttcgagaggc
     2881 cccccgcgcc cacgtcgtga atgaacgcga tgggagacgc ttcccccaga gcattgcagc
     2941 gatcgatgac ctcctgacag cgccgttgca tctcggcgtt ttcccgttgc accgaggcaa
     3001 aatccaaatc ggcgctgctc gcgcccgacg ccacggaaga ggccgcaccg ccgcccaaac
     3061 caatcagcat cgccggcccg cccaacacta ccaaacacgc accgggcgcg aatggccgct
     3121 tttgcacgtg catcgggcgc acgttgccga gcccacccgc caacatgatc ggtttgtgat
     3181 acccacgcac cacgtcgccg tcggggagct cgaaggtcct gaagtagccc aagattgctg
     3241 ggcgcccaaa ctcgttgttg aatgcggcgc ctccgattgg gccctcgatc atgatatcga
     3301 gggccgacgc aattcgatcg ggcttgccta cggggttggt ctcccaaggc tgttcgaagc
     3361 cggggatgtg caagttggag acagagaatc ccgtcaatcc aactttcggt ttcgcgccgc
     3421 gcccggtcgc gccctcatcg cggatctcgc caccagaacc ggttgccgca cccgggaatg
     3481 gcgagatcgc cgtcgggtga ttgtgagttt cacacttgat gaggatgtga acgtcttccg
     3541 cgacttcgcc gtaaactccg ctatgcgggt ctgggaacag ccgcaagccc ttgctgcctt
     3601 cgaacacggc cgcgttgtcg cgataagcag acagcgtgcc ctcgggccgc agctgatgcg
     3661 tattctgaat catcttgaac agcgagtgcg gttgcttcac gccatcgatg atgaaatcgg
     3721 ctttgaagat cttgtgccgg caatgctcgc tgttggcttg cgcgaacatc atcagctcca
     3781 cgtcgctagg atttcgcccg agctcctgga aagacgtgac caggtagtcg atctcgtctt
     3841 cggccaacgc gagcccgagc tcggcattgg cacgttcgag cgccggacgc ccttgcccga
     3901 gcacgtccac gtggcgtacg ctgcgcgcgg cctcgtggcg gaaaagcgaa cgcgcttcgt
     3961 cgggcgagcg caataccgtt tccgtcatgc ggtcgtgcag cagggtcatg aaagagctca
     4021 cccaatcgat gctcaccgca ccctcgaagc gataggcggt gccacgctcg atgcgcccga
     4081 cctcgggcag gccgcacacg tgcaggatgt cggtggcctt cgaagaccag ggcgaaatgg
     4141 tacccagccg gggcaccacg atcagatggg cggcgcgctg agctgcctcc gccgagctgg
     4201 cctgcagcgg tgtggcctcc acgatcgaca ataggcggtc gagcttgaac ggctcgatcg
     4261 cgctttgagt ttccacgaag taccagtgct ccgcgctcaa cccggtgagt gagggctgga
     4321 gcttcttcag ctcttcgagt ttgcgagcgc gccgaaacgg ggtgagcgcg gcagctccgc
     4381 gcaaaacgag catctgagac acggaaaact ccttggcgct ggtagcgccc gagcgcttct
     4441 ttgcagagtg ccggcagtgc ggcaacccca cgcgagtcga ggcggcgcca aggccgaatc
     4501 gggctacccg ccgagcggcc gaagtgtacc cgggaagctg cccatcgtga cctaccgcgg
     4561 ctcgaatttc ccgagcgtca cggggcggag tcggccatgt gcgcaagctt cacccaagtc
     4621 tcttcaaaag cgccgtcgaa gcgccaccca acgcgcctcg gccccaacgc ttttcgtcat
     4681 gttcgagctt attgggttgg ctcactgacc gtcctagccg tctgcgtgtc accgggtgcg
     4741 atcgtcaaaa ccgcgagtga gaagactcgg accgagggag ctcacgaagg gttcagcctg
     4801 cggtgcgggg ccaaacatcg gcgcgcagcc ggcacgggac tgctggcatt acccctgtgg
     4861 gtcatgagcg gttgcgtcct gagcgatccg ttcttcatcg atcccagcgc gagtgaaggc
     4921 ggcgccagct ctcagggcgg gacgggcggc gcgagcgaga cggcgcgcgg cggcaccact
     4981 cctaagggtg ggaacacgtc gggcggcacg agtccagttg gccccaaagc gggcggcccc
     5041 gggttgggtg gctctcctct gggaggcacc ccgagcacga cgtcggtgca aggcggattc
     5101 accggcacga cgggccacca gggtggttct ggtggcgcca gcaacactca ggccggtggt
     5161 gcagggggca ccgtccaaag cagcacgatc caagcctccg gcgggagcgc cggcaactgg
     5221 atattggctg atttcgagac aagtacggag ggcttcgggc caccaccaga gaagcagaac
     5281 tccaacaccg tctcgaccac cgccttctat catactcagg gaaagcaagg gctcttggcg
     5341 accatgcgca gcgccgagtg ggtcggagcc atgtttcctc aagcgctcga cctctcacgt
     5401 gccaatcgaa tcaaggtcga tatccgcacc agcaccgcgc cactccaagc cgacgtcgcg
     5461 atcgtgacgg gatcagacag ctggtgttcc gggaatatca tgattcaggt ccccgcccag
     5521 acggcgacta cgctcgaggt aaacctgaag accagaacct gcgccaactt cgacgcgagc
     5581 cgcgtgcgcg gagtctacat ctggttcgag gtaggcacgt actacgccga ctacatgagg
     5641 accgagtaaa gcagccatga cggagacggt cgattcggac aaggccttac gcttgcgctc
     5701 ggaaccggcg gcgcccgcgg cagagccgag ctcctacctg cagggtgagg tcatcggcga
     5761 tcgctatcgg ctggagcgtg agctcgggcg tggaggcatg ggtgtcgtgt gggtggccca
     5821 ctcactggtg cttggcatcg acgtcgcggt gaagctgatc cgagccgaaa ccgcggggcc
     5881 cgcggtggcc tcccgcatgg ctcgggaggc gcatgcggcc gcacgcctcg gtcatcccgc
     5941 cctggtgcgg gtattcgatt ttggttggac gggccgcggc gacccgttct tggtgatgga
     6001 gctcgtccaa ggggaaaccc tctccgagat gatccgtcgc gagtgtcagg ttcccgtgat
     6061 tcgcgcggtg cagatcctgc tcccgatcgc ggatgggctg cgcatagcgc acgaccgcag
     6121 catcgtgcat cgcgacatca agcccgacaa tatcttgctt tcgaccgact cgctggggcg
     6181 gcttcaaccg aagctgctcg acttcggcat tgccaaggtg gggcccacgg tcggggaagg
     6241 caagcttacg cagttcggta cggtgttggg tagccccgaa tacatgtctc ccgagcaagc
     6301 actcggcttg gatgacatcg acgagcgcac cgatgtttgg tccttttgca gcgcgctcta
     6361 cgagatggtg actggttcga ttccgttcgc caaatcgaac tacaacgcgc tgatgcacgc
     6421 catcatcagt gaggagccac agtctaccgt gagccaaggc gtgggagacg ccgccttctg
     6481 ggccatcctc gagcggggcc tgaccaagga gcgggacaag cgcttccgca acatgacgga
     6541 gctcggcgaa gctttggcgt tatggcttta cgagcatgga atcaaggccg attcctgcgg
     6601 caactcactg cgcgccgttt ggttggacgt gagcatgggc accctgctcc ccgccgtcga
     6661 attgtccatt ccacccggca gcgtcatcgc caattcgtct tctctgtcgc ccgcgatcgc
     6721 ggcccgcacg acgcg
//
LOCUS       DASTKU010001056         8433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_654281, whole genome shotgun sequence.
ACCESSION   DASTKU010001056 DASTKU010000000
VERSION     DASTKU010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654281"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1382)
                     /locus_tag="VFQ61_37520"
     CDS             complement(171..1382)
                     /locus_tag="VFQ61_37520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960269.1"
                     /translation="MTEQKPKIDLKARLGKKTTAGAPAAGGSIPPPVAASSPSAAAPA
                     AVPGGGLPSAIPGGPAPAMRPAMQRPSAAPAVSPSGIPAPPSFGGGAASSPFAGASNP
                     YAAQAAQPAPVAAKPQAIRIEMGEEVVEAQRRGRKKVAVLAAVTAIVGGVIGFAAGGG
                     AERAKGAEAAVVGAQDLIKEIGAANAEVQKLADTVKSAKEKLGKGQYPEEEVSKLGGI
                     NIPFTGKNLAGKGIGRFRPEIVTMLIEYANSTTEANSQKEKLQNVLSGTKKGILELLG
                     EPKVRWGVVLGNGPNGPWGSMQPLPAAFGPKEKWPDDIKVGSGKEQSTLKRYASGNPI
                     NNDAPFFVPVDPSSQSSVCPADVVFKLRRELSDLESVLRGDNTPGEDRKGLLESGQQL
                     TNKLKQIGKES"
     gene            1808..2845
                     /gene="trpD"
                     /locus_tag="VFQ61_37525"
     CDS             1808..2845
                     /gene="trpD"
                     /locus_tag="VFQ61_37525"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET9960270.1"
                     /translation="MTSNTPVFAEIFEELTSASGLAPSRVREVFDSIFDGKWTPAQIG
                     GLLVALRMKGETAETLAAAAAAMRAVMLRVDHGLPLVLDTCGTGGDRSSSLNLSTGAA
                     VIAAAAGVPVAKHGNRAATSRAGSADVLEALEIPLNVPVSGQATVLKTAGITFLFAIA
                     HHPAMRHAMPTRRELQIRTLFNCLGPLANPAGATHQLIGAADNATRALMSQALRELGT
                     ERAWVVRSADGLDEFSPSAPTHVTQVSGGQVEELTLAPEDFGLERQDRSSIQGGDTAF
                     NAAALSRVLRGEPHPARTAFILNAAAALVVAKGLTPKAAADLAQEVTTSGAAFQKLEA
                     WRDVARAAVNA"
     gene            2854..3618
                     /locus_tag="VFQ61_37530"
     CDS             2854..3618
                     /locus_tag="VFQ61_37530"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET9960271.1"
                     /translation="MGILDEILSHKVAEVERLKASTLPSGPGARGISLARNAGEPLRL
                     ITEIKRRSPSAGALSTKLSVAERARCYADAGASMLSILTDSRFFDGSFEHLREARAAC
                     DLPLLCKDFVIHERQLDAALAFGADAVLLIVRCLSESALPRLHAAAKSRGLAVLVEVT
                     DEPESQRALDAGAEWVGVNVRDLDTLAMDSARAARVLAMLPKTVQRVHLSGLKDAASV
                     LKVAQSGMDAALIGEALMRLDDPSSLLASMRAAAAS"
     gene            3681..4574
                     /locus_tag="VFQ61_37535"
     CDS             3681..4574
                     /locus_tag="VFQ61_37535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HET9960272.1"
                     /translation="MALDTWIDVYLTHLRVERSLSPHTVAAYSRDLAKLVSFAEQHGV
                     GEPRELDLGTFSGWLTSLSKGGLGARSAARHLSAARGFVKFLVREGEIPDDPTRLAAR
                     PRIGRRLPSALGEAELRALIEAPDPNTLRGLRDRAMLSVTYASGLRASEVVSLTLGDL
                     DLRRGIVSALGKGNKRRLVPLGEIALLHLDAYLTARRAAEPVEQSSAIVFRSPRGGSL
                     TRQGFWKIVKQYALAAGLKDRVYPHKLRHSFATHLLGGGADLRSVQTLLGHQNIATTE
                     IYTHVSRDHVRKAHRRSHPRA"
     gene            4631..5500
                     /locus_tag="VFQ61_37540"
     CDS             4631..5500
                     /locus_tag="VFQ61_37540"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9960273.1"
                     /translation="MLSRIRIAHDGPALRRLAYWGARYGPAFWVKHSPAFFGVAFALA
                     LPAQRHKVQRSLRLARGPVGPAQERFETLATFVRYAQCLAEGLAVERKEALNAQHRVI
                     NEDTLQAALAQGRGLVITTAHAGPWDAAAQLLYRALGREVLVVMVGERDARARELHDA
                     VRARSGVRIIHVGEHPLDALPVLHALRGGAVVAVQLDRGAPSGRGIDVQLFGGAAQVP
                     EGPFRLAALSGAPIVPLFVRRRAAFNYEFEVHPVIQLPRGASPTELGAAAQRAVDAMA
                     EFIRSEPTQWFDF"
     gene            5691..6965
                     /locus_tag="VFQ61_37545"
     CDS             5691..6965
                     /locus_tag="VFQ61_37545"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HET9960274.1"
                     /translation="MPDPSSHPAAGDDALFYDWNRSERPVREVGVVDETLRDGLQSPS
                     VQDPSTPQKLALLQQAVALGVDGFALGFPASRPRQFEDALRLAQEAANQRFEVKLCCA
                     ARTLIADIEPVARIAQRAGVLIQVGMFIGASPLRQMAEGWSLSRALTLTEDAVRFAAR
                     EGLEVLYVAEDATRTPPATLEQLYRTAIACGARRVCVADTVGQATPEGAENLIRFVRQ
                     LVDGIDPRIAIEWHGHRDRGLAVANCLAAWRAGAELCHGTAFGVGERCGNPPIELLLT
                     NLALLGHRKFDLSHLPDYAHAAQTALGVPIPPNQPVVGRDAFRTATGAHAAALGKAMS
                     RSDTWLADRVFSAVPASWVGRLQRIEIGPASGEANVLAWLSAHAVSPSTELVRAILQA
                     AKSRSRLLDDAEILAITRETRPGARTASSKSR"
     gene            7149..8123
                     /locus_tag="VFQ61_37550"
     CDS             7149..8123
                     /locus_tag="VFQ61_37550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960275.1"
                     /translation="MRLLVGLTSVVGSVENYARHFDLLELRADPERLPAPKVLKKQAS
                     LAPALTTSILMPPRWVAKALEDPNQASPLLPLLEAANASWLILQTGPEIGPSRRAAGR
                     LATLCQYFSGKVEQIGWEPHGPWEPETALAEAQKLGIVWVNDRSVEEGSRGPKIYTRL
                     RSLGPGASLRPTALDHLGLELVMAETACVVFEGQPGGRARERVQQAVQAALEAVQQQL
                     EEEDELEDDDDFEDEDELEDTDDEGPAEADDADADDADAESPSEESDDEDSDESDDED
                     SDESDDEDSDEFDDEDSDEFDDEDSDESDDEDSDESDDAPAPKPRGRS"
     gene            8123..>8433
                     /locus_tag="VFQ61_37555"
     CDS             8123..>8433
                     /locus_tag="VFQ61_37555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960276.1"
                     /translation="MRIRHAVAASDSIAEQAATERLAAGGSAVTAVLAGFFAVAGARP
                     GVLLGPMSILVAGVGQGARAFDGRVRQPGLGAKRPRGILDGSEVPEAAQVAVPGALGA
                     AL"
BASE COUNT         1404 a         2707 c         2777 g         1545 t
ORIGIN      
        1 acggaaagga cccaagcccg gcgctgctcg cgggcggggc cgtcgcggcc tggtgcgctt
       61 gctgagtggc acgaacagga gcccggttct tagcgaagcc aggctcctgg agccgtccca
      121 gagctgctga ttcagtgggt cggcgttgaa ccaacgccga ccccggctaa tcaggactct
      181 ttgccgatct gctttagctt gttcgtcagc tgctggcccg attcgagcag ccccttgcga
      241 tcctcgcccg gcgtgttgtc accgcggagg acgctctcga gatcactcag ctcacgacga
      301 agcttgaaca ccacgtctgc cggacagacc gacgattgcg agctcggatc taccggcacg
      361 aagaaggggg catcgttgtt gatcgggtta cccgaagcgt agcgcttgag cgtggactgc
      421 tctttgcccg aacccacctt gatgtcgtcg ggccacttct ctttgggccc aaatgccgcc
      481 ggcagcggct gcatgctgcc ccacggtccg tttggaccgt tgccgagcac cacgccccag
      541 cgcaccttcg gctcacccag caactcgagg atgcccttct tcgtgcctga cagcacgttc
      601 tggagcttct ccttctggga gttcgcctcg gtcgtcgagt tcgcgtactc gatcagcatc
      661 gtcacgattt ccggacggaa gcgcccgata cccttaccgg cgaggttctt acccgtgaac
      721 gggatgttga tcccgccaag cttcgagacc tcttcctccg gatactggcc cttgccgagc
      781 ttttccttcg ccgactttac ggtgtccgcg agcttctgaa cctcggcgtt cgcggcgccg
      841 atttctttga tcaagtcttg tgcgccaacg acggcggctt cagcgccctt ggctcgttcg
      901 gcgccgccac cggcagcgaa accaatcact ccacccacga tcgccgttac ggccgcgagc
      961 actgccacct tcttgcgtcc gcggcgctgg gcttcgacga cctcttcacc catttcgatg
     1021 cggatcgcct gcggcttggc ggcgaccgga gcgggctggg ccgcctgcgc agcgtaggga
     1081 ttggacgctc ctgcaaaggg tgaagaggca gcgcctccac cgaatgaggg cggggccgga
     1141 ataccagacg ggctaaccgc gggagccgcg ctgggccgct gcatggcggg gcgcatggcc
     1201 ggggccggac caccgggaat ggcgctgggc aatcctccac ccggcaccgc cgctggcgcg
     1261 gctgcggatg gagaggaggc ggctacaggc ggcggaatag atccgccggc agcaggcgca
     1321 ccggccgtcg ttttcttacc gagccgtgcc ttgagatcga tcttgggctt ctgttccgtc
     1381 atcggggttc gttcgccacg gggaggagaa ccgccagtcc gctcggctgg cggccagtgg
     1441 gacgggcggc acagtagcgg cccactaact cgccggcaag attgaaaaag cgcaaggggt
     1501 gctgaagtaa ggggcggttc gccgcaaaat gccgctcgcg aacgaaagag cggccgtttt
     1561 ggtgagtgcg ggccagttgc agaatgttcg cgaagcatga tctccgtctg cacttggcag
     1621 acgtcggagg tcccggcgtc aatgcgcgtc ggcagcgaga gagaggtctg acgacggcga
     1681 ggcagcgtgc cggccgatcc gcttcgtagt aaaagaagcg ccgtttttgt cgccggcagc
     1741 ccaatcattg ccgagcttga ctcgatctcg aaccaatctc gaataagctt caagccctgg
     1801 atcgcgcatg acctccaata ctcccgtatt tgccgagatt ttcgaagaac tgaccagcgc
     1861 cagcggcctg gctccgagcc gcgtacgcga ggttttcgac tcaatcttcg acggcaagtg
     1921 gactcccgcc cagattggcg ggttgttggt cgcgctcagg atgaagggcg aaacggcaga
     1981 gacgctggcg gccgcggcgg cggcgatgcg ggccgtaatg cttcgcgtcg atcacggatt
     2041 gccgctcgtt ctggacacct gcggtacggg tggggatcgc tcgtcgagcc tcaacttgtc
     2101 gacgggggcg gcggtgatcg cggccgctgc cggcgtgccg gtcgccaagc acggcaatcg
     2161 agccgccacg agccgagccg gcagcgcgga tgtgctggag gcgctcgaaa ttcctttgaa
     2221 tgttccggtt tcgggccaag caaccgtgct caagaccgcc gggattacgt tcctcttcgc
     2281 gatcgctcac catccggcaa tgcggcacgc aatgcccact cgccgcgagc tccaaatccg
     2341 gaccctcttc aattgcctgg gtcccctcgc caatcccgcg ggagcgacgc atcaattgat
     2401 cggcgcggcg gataacgcga cccgcgccct catgtcgcaa gctctgcgtg agctgggcac
     2461 ggaacgcgct tgggtcgtcc gcagtgccga tgggctcgat gaattcagcc cgtcagcacc
     2521 cacccacgtt acccaggtgt ccggcgggca agtggaggag ctgaccctgg ctcctgaaga
     2581 ttttggcttg gagcgtcagg accgcagctc gatccaaggc ggggatacgg cgttcaatgc
     2641 cgccgcgcta tctcgggttt tgcgcggtga accccacccg gcccgcactg cgttcattct
     2701 gaacgcggcg gccgcgctcg tggttgccaa gggactcacc ccgaaggctg ccgcggactt
     2761 ggcgcaagaa gtcaccacct ctggcgcagc ttttcagaag ctcgaggcgt ggcgagacgt
     2821 cgcacgcgcc gccgtcaacg cttgagctgt accatgggca ttttggacga gatcctcagt
     2881 cacaaggtcg cggaagtcga gcgactgaaa gcttccacgc tgccgagcgg gcctggcgct
     2941 cgcggcatct cactcgcgcg aaacgccggc gagccgttgc gtttgattac cgaaatcaag
     3001 cgtcgatcgc cttccgccgg agcgctttcc acgaagctat cggtcgccga gcgggcccgc
     3061 tgttacgccg atgccggcgc gagcatgctc agcatcctta cggattcccg cttcttcgac
     3121 gggagctttg aacatctacg cgaagcccgc gccgcctgcg acctgccgct cttatgtaag
     3181 gacttcgtga tccacgaacg gcagctcgac gcggctctgg cctttggcgc tgacgccgtg
     3241 ttgctcatcg tgcgttgcct atctgaatct gcgctgccgc ggctgcacgc tgccgcaaaa
     3301 tcacgcggac tggctgtgct ggtggaggtg accgatgagc cagagagcca gcgcgcgctc
     3361 gacgcgggcg cggagtgggt cggcgtgaat gtgcgggacc tcgatactct ggcgatggac
     3421 tcggcgcgtg cggcgcgggt gctcgccatg ctgcccaaga ccgtccagcg tgtgcacctc
     3481 tcgggtctca aggatgcggc cagcgtcctc aaggtggcgc agagcggtat ggacgccgcc
     3541 ttgatcggtg aggcgttgat gcggctcgac gacccatcct ctttgctcgc cagtatgcgc
     3601 gcggccgccg cgagctgagc gccgttgagc caacacacca aattgcgccg attctggccg
     3661 gacgcggtac agtgtggcgc atggctctgg acacgtggat tgacgtctac ctgacgcatt
     3721 tgcgtgtgga acgcagtctg tccccgcaca ccgtcgcggc ctattcgcgc gatcttgcca
     3781 agctcgtctc cttcgccgaa caacacgggg tcggcgaacc gcgagagctc gacttgggta
     3841 cgttcagcgg ttggctcacc tcgctatcga aaggcgggct tggagcccgc agcgccgctc
     3901 gccacctctc ggcggctcgt ggtttcgtga agttcctggt gcgcgaaggc gagatcccgg
     3961 atgatccgac gcgactagcc gcccgcccac ggatcgggcg gcgtctgccc tccgcgctag
     4021 gcgaagctga gttacgcgct ctgatcgaag ctccggatcc gaacaccctg cgcggcttgc
     4081 gagatcgcgc catgctgagc gtgacctacg cttctggtct ccgcgcgagc gaggtggtct
     4141 ctttgacgct gggggatttg gatttgcggc gcggcatcgt ctccgccttg ggcaagggta
     4201 ataagcggcg cttggttccg ctgggcgaaa tcgcgctgct tcacctggac gcttatctca
     4261 ccgcgcgacg cgccgcggag ccagtcgaac agagttcggc catagttttt cgatctcctc
     4321 gcggcggttc actcacgcgc caagggttct ggaagatcgt caagcaatac gcgctggcag
     4381 cgggcttgaa ggaccgggtc tatccgcaca agctacgtca ttcgttcgct actcatctac
     4441 tcggtggagg ggcagacctc cgatccgttc agaccctgct cgggcatcag aacatcgcca
     4501 cgaccgagat ctatacgcac gtttcccgcg atcacgtgcg aaaggcacat cggcgctcgc
     4561 atccacgcgc ctgatcccga tccggggcgg ggcttgccag ccaccgcatg ttcatgagag
     4621 gcgagacgcg gtgctctctc gcattaggat tgctcacgac ggcccggcgc tgcgacggct
     4681 cgcgtattgg ggcgcgcgtt acggcccggc gttttgggta aaacacagcc ctgcgttctt
     4741 tggcgtggcg ttcgcgctcg cgctgccggc ccaacgtcac aaggtgcagc gttcgttgag
     4801 gctcgcgcgg ggcccggtgg gccccgcgca ggaacgattc gagaccctcg cgacgttcgt
     4861 gcgttacgcg cagtgtctgg ccgaaggcct ggccgtggag cggaaggagg ccttgaacgc
     4921 gcagcatcgg gtgatcaacg aggacacgct gcaagcggcg ctcgcgcagg ggcgtggcct
     4981 cgtgatcacg acggcccacg cgggcccctg ggatgccgcc gcccaacttc tgtatcgcgc
     5041 cctcgggcgc gaagttctgg tggtgatggt gggagagcga gatgcaagag cgcgcgagct
     5101 acacgacgcc gtgagagcac gctccggcgt ccgcatcata cacgtgggag aacatccact
     5161 agatgccttg cccgtgttgc acgcgctgcg cgggggtgcg gtcgtcgcgg tgcagctcga
     5221 ccgcggtgcc ccgagcggtc gaggcatcga cgttcaactg ttcggtggag ccgcccaggt
     5281 acccgaaggg ccatttcgtc tggccgcgtt atcgggggcg ccgatcgtgc cgctcttcgt
     5341 acggcgccgc gcggcgttca actacgagtt cgaggttcat ccagtcatcc agctaccgcg
     5401 cggggcgagc ccaaccgagc ttggggccgc cgcccaacgc gcggtggacg cgatggctga
     5461 gttcattcgc agcgagccca cgcagtggtt cgacttctga ttgccggcac cccgaagacc
     5521 tactccgcaa gagacgcaac ggaagagacc tcggagccca acgtccgatg atgctttttg
     5581 gcccggtgcg ttcgggtcac gttcgccggg gcacctttag ctcgggtgtg tgcgagtttt
     5641 tggccgtctc gaccctcccg atggtgctaa tccttggttg acccctgcgc atgcccgacc
     5701 cctcgtccca ccctgccgct ggagatgacg cgctgtttta cgactggaat cgcagcgagc
     5761 ggccggtgcg tgaggtgggc gtggtcgacg agaccctgcg agatggtctg cagtcgccat
     5821 ccgtgcagga cccgagtact ccgcaaaagc tggcgctgct ccagcaggcg gtcgccctgg
     5881 gggtcgacgg ctttgcgctc ggcttccctg cgtcgcgccc gcggcagttc gaggacgcgc
     5941 tccgtctcgc gcaagaagct gcgaaccagc gcttcgaggt aaagctctgc tgcgcagccc
     6001 gcaccttgat cgcggacatc gaaccggtcg cgcgcatcgc gcagcgcgcc ggagtgttga
     6061 tccaggtggg catgttcatc ggtgcgagcc cgttgcggca gatggcggaa gggtggtcgc
     6121 tgagccgcgc gctgactctc accgaagacg ccgttcgttt cgcagccaga gagggtttgg
     6181 aggtcttgta cgtcgcggag gacgcgaccc gcactcctcc cgctacgctc gaacagctct
     6241 atcgcaccgc aattgcatgc ggcgcgcggc gcgtgtgcgt ggcagatacg gtcggtcagg
     6301 ccacacccga aggtgctgag aacttgattc ggttcgtgcg ccaactggtc gacggcatag
     6361 atcctcggat cgccatcgag tggcacggcc accgagaccg cggcttggcg gtggccaatt
     6421 gcctggcggc gtggcgcgcc ggggcagagc tctgtcatgg cacggcgttt ggtgttggcg
     6481 agcgctgcgg caatccccct atcgagctct tgctcaccaa cttggctctg ctgggacacc
     6541 gcaagttcga cctgagtcac ctgcccgact acgcccacgc ggcgcaaaca gctctcggcg
     6601 tgcccattcc gcccaatcaa ccggtggtcg gcagggatgc gttccgcact gccacgggcg
     6661 cgcacgcggc cgcgctcgga aaagcgatgt ctcgctcgga tacgtggctc gcagatcggg
     6721 tattttcggc cgtacccgcg agctgggtcg ggcgcctgca gcgaatcgag atcggccctg
     6781 cgagcggaga agccaacgtc ttggcttggc tctcggcgca cgcggtgtcg ccctcaacgg
     6841 agctcgtccg cgccatcctt caggccgcca agtctcgctc ccggctcctg gatgacgctg
     6901 agatcctggc gattacacgg gagactcgac ccggcgcgcg tacggcgagt tcgaaatcga
     6961 ggtagtgaga gctcccgacg cccattccaa cgcgggtgag gtcggatgag tttcggcggt
     7021 ggggtggagc ttcgcggccg cctcgagtca cactggcgat ccgcacggcc gtttctggtg
     7081 cttgactccg catttttgac cgagctcctt gcaaggtcgc ccagggcgtg agaaagcctc
     7141 gcgcccccat gcgcctgctc gttggtctca catcggttgt tggtagtgtc gaaaactacg
     7201 cgcggcactt cgatttgttg gagctgcgcg ctgatcccga gcgtctcccc gcgcccaaag
     7261 ttttgaagaa gcaggcctcg ctcgcgcctg cgctcacgac cagcattttg atgccgccac
     7321 gttgggtcgc gaaagcactc gaagatccca atcaagcgtc gcctctgctc ccgctgctgg
     7381 aagccgccaa tgcaagctgg ctaattcttc agacggggcc agaaatcggg ccgagccggc
     7441 gagcagccgg gcgccttgcg accctgtgcc aatacttttc gggcaaagtg gagcaaattg
     7501 gctgggaacc gcacggcccc tgggagccgg aaaccgcgct ggccgaagcc cagaaactcg
     7561 gaatcgtgtg ggtgaatgat cgcagtgtcg aagagggatc gcgcggccca aagatttata
     7621 cgcgccttcg ttctctgggc cccggcgcaa gcttgcgtcc cacggccctc gatcacctcg
     7681 gtctcgagct cgtaatggcg gagacggcgt gtgtcgtctt cgaagggcag cccggtggtc
     7741 gtgctcgaga gcgagtgcaa caagctgtcc aggcggcgct cgaagccgtt cagcaacagc
     7801 tcgaggaaga ggacgaactc gaagacgacg atgacttcga ggacgaagac gagctcgaag
     7861 acaccgacga tgaaggcccg gcggaggcag acgacgcgga cgcagacgac gcggacgccg
     7921 aatcgccgtc agaagagtcc gacgacgagg attctgacga gtccgacgac gaggattctg
     7981 acgagtccga cgacgaggat tctgacgagt tcgacgacga agattctgac gagttcgacg
     8041 acgaagattc tgacgagtcc gacgacgaag attctgacga gtccgacgac gccccagccc
     8101 ccaaacctcg gggacgctcc tgatgcgcat ccggcatgcg gtagccgcta gcgacagcat
     8161 cgcggagcaa gcagccaccg aacgtcttgc ggccggcggc agcgccgtta ccgctgtttt
     8221 ggccggattt tttgcagtgg cgggcgcccg cccgggcgtg ctgctcggcc ctatgtcgat
     8281 cttggtcgct ggcgtgggac agggagctcg agctttcgat gggcgtgtgc gccagcccgg
     8341 tttaggggcg aagcgcccgc gtgggatcct ggacggctcc gaagtgcctg aggccgcgca
     8401 ggtcgccgtg cccggagcgc tcggcgccgc gct
//
LOCUS       DASTKU010001057        10105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_430720, whole genome shotgun sequence.
ACCESSION   DASTKU010001057 DASTKU010000000
VERSION     DASTKU010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430720"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VFQ61_37560"
     CDS             complement(<1..889)
                     /locus_tag="VFQ61_37560"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HET9960277.1"
                     /translation="MNRLAHETSPYLLQHAHNPVDWFPWGEAALSKARSEDKPIFLSI
                     GYAACHWCHVMERESFENARVAQFLNEHFVSIKVDREERPDLDDVYMAATVALSGSGG
                     WPMSVFLSPDTRPFFAGTYFPPDDRYGRPGFLNLLQRIHELWKSQRESLLGQAAELTE
                     HVRQELGITAPAAIASESFTRVVRDFSQSFDAQYGGFGRAPKFPPHQGLRLLLRLYDK
                     SRDPTLLTMLRTTLDGMKNGGIYDHLAGGFARYSTDERWLVPHFEKMLYDNAQLTVVY
                     LEAFQLTGDSEYARVARETL"
     gene            complement(1017..1700)
                     /locus_tag="VFQ61_37565"
     CDS             complement(1017..1700)
                     /locus_tag="VFQ61_37565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960278.1"
                     /translation="MNAPQGFVVRTGNPEQDRYEVARYQQHYATQGLHVAAEPLAEGG
                     FLLRVYPARPSVPPPEAASARGVPRTQGMQRVVSQSFGPDGSTQDAAAFGFVGFGGAA
                     GGLPEPAAHSPFPGAAALPRDFSSSPGAATLASGPWAAGGAARPSAPPQSPQSPQSPQ
                     SPRPSPSVPPTVGFAVPDVPNVVGVPAVPGRPRSAARVLLILLLGAVLGAGLGYLALR
                     YSHQLGWLG"
     gene            1882..2679
                     /locus_tag="VFQ61_37570"
     CDS             1882..2679
                     /locus_tag="VFQ61_37570"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET9960279.1"
                     /translation="MLHFDGKVVLVTGGSSGIGRAAAQAFADAGAKVILTARGRELGE
                     ETAAAIRQRGGNVSFVRADVSRATDVKTVLGEIVSRHGRLDAAFNNAASDVGMFQLTA
                     DFNEAQFDECMQLNLKSIWLCMKYEIEQMLKQRPRGGAIVNTSSVSGLGGSPSGALYS
                     AAKAGVLALTKAAAQEYATSGLRVNALVAGAFRTPMLKRVFTRASGGTPEGMAQVEDR
                     YVSLIPQGRIGAPEEAANAAVWLCSDAASYVTGHSMIVDGGMTSMLR"
     gene            complement(2700..3932)
                     /locus_tag="VFQ61_37575"
     CDS             complement(2700..3932)
                     /locus_tag="VFQ61_37575"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HET9960280.1"
                     /translation="MSAREPPIRPRRALTHVGGEVDVVARDPELGEALRRALDVDPDE
                     DRVRAHVHGFHSYPARMHPDTALALIATLSSPRATVLDPFAGSGTVVVSARELGRRAI
                     GSDLNPLSVALCRVKTTGCPPGFAEALLAAAERVVEHAKERQAKKLGPTHPYGPEDRQ
                     LYETYVLLALDGLRDGIERAAPEETRRTLFLVLSSMLTKLSRKRGDSSDGDQPKRLAR
                     SFAFRLFLLRTRELVEQLRTFTELMPPGTPPARVEQCDARQLGFLKSGSVDLVVSSPP
                     YPGVYDYFAHHSARLRWLRMPGEDFRRGELGARRAFERDSQRGLQGFERDFTHCLSEL
                     ARVITREGRVALLIADSAVSGRPLFVGEWLPPLVDRAGLRLIARASQTRPNFHEPSSR
                     AFTQRDRREHLVLLARDT"
     gene            4081..4902
                     /locus_tag="VFQ61_37580"
     CDS             4081..4902
                     /locus_tag="VFQ61_37580"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET9960281.1"
                     /translation="MLLVIDIGNTNVVFGLYDGETLTHTFRMATVHTRTEDEYGALLL
                     QLLQLRGLPVNCVECAILASVVPPLTDVMIDAVRSAFAREPLVVGPGLKTGIPVLYEN
                     PRDVGADRVVNAVAAFERYKEATIVVDFGTATTFDCISPKGEYLGGVIVPGMRISLDA
                     LLDRAAKLSRIELSAPPRVLGRNTTHALQSGLIFGHASMVDGMIERLEQEAGYPSRVI
                     ATGGLSGLVSKHARRIEAVDVNLTLEGLRILHERNVQGADSRANNVKRSRAPKNS"
     gene            4932..5798
                     /locus_tag="VFQ61_37585"
     CDS             4932..5798
                     /locus_tag="VFQ61_37585"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HET9960282.1"
                     /translation="MGALLACGSRGAGSVALGSGMACSKQRAPSRGRTRIVSLAPDLT
                     HTLFSIGAGELVVGVSDYCQDPEAVQRLPRVGTSLTPNYEAITRLEPTLILSEDNRSA
                     RKRELSAIARAEFLPWLSLAEVAASIRVLGRLTQHGREANQLAERLVSVLGVPPPTAG
                     PRVLLVLGDGADAPGELWFVRQNSLHGAALNAAGARNAVPEAVLAAPRLSYERLVQLD
                     PDAILLLCLPNRAAERATTIGTLQRFETLSAVKRRRVGILESEAAFANGPRILTLVPA
                     LRDRLKALELVP"
     gene            5795..6595
                     /locus_tag="VFQ61_37590"
     CDS             5795..6595
                     /locus_tag="VFQ61_37590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9960283.1"
                     /translation="MTAALRVQGVGVKLAGRLVLDAVSLELDPGRLLALVGPNGAGKT
                     TLLRAALGGLPWCQGEVLLGGRSLRSYTPRERAAWLGFLPQQLASPEPLPVLEVVQTG
                     RFRFDESESASRRAALLALERAGISALRDARLTELSGGERQRVAMATLLAQEARLALL
                     DEPASHLDPAQQIDSYGLISELLERGLGVLLVTHDINLLSHIAIPDRVRVLGLARGRS
                     MFELAFQDPALPGALTRLFGVRFHEFDGQGKRLLLALERVEGESEPLK"
     gene            6592..7539
                     /locus_tag="VFQ61_37595"
     CDS             6592..7539
                     /locus_tag="VFQ61_37595"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET9960284.1"
                     /translation="MSSKPRVLLLSAASLAVMAGSLFVGPAFDGPHASFVLLELRLPR
                     LLVAMLVGAALALSGAAFQILFNNPLATPSTIGTLSGATLGALASIVLGVDAELWGLS
                     ATTLCAFVGALLTSLFALGVAVSGRARMSDVLLAGIAIGLSTSALATGLEYAADSRMV
                     FAASRWSLGQLPQVGYRGVWLLLPIVVLTTAVTLSQTRALSSFMLGEEMARAQGVHIG
                     RLRSIVLVNASLAVAAVVAWCGPIAFVGLIVPHLVRLSVGASLRLVLPLSVVVGAGFL
                     AFADGLSRALLPGRELPVGVLTAALGAPALIWLLARQRR"
     gene            complement(7604..8380)
                     /locus_tag="VFQ61_37600"
     CDS             complement(7604..8380)
                     /locus_tag="VFQ61_37600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960285.1"
                     /translation="MLSVRISAGRMPSTQRAAESALKIAVLIGLASACGKKDAETGGY
                     QQGQVQYNQQNPPGGYGGQGYAGYPAQGYGGVGGQANVPPGYGTAGVGGVGGAPSGIP
                     GAGAPAGGVGGALPGTTGAKAQQLDPAAAAVVQPVINELSKQHIVAGSKPLGSVVAGS
                     FQTGQTLEAQLQLQPQKCYSIVATALPPVTELNVQLVATTPLPNLSPVLATDSDTGPT
                     AVIGRKPNCYKWPFPLAAPVKVVIQVAAGSGLAAAQIFEK"
     gene            complement(8484..>10105)
                     /locus_tag="VFQ61_37605"
     CDS             complement(8484..>10105)
                     /locus_tag="VFQ61_37605"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9960286.1"
                     /translation="GVDVPLLYHAFFGPSVLHPGMIEDIELYAGAAPVNLGRFAGPIV
                     AARTRPLSHQLRGEASIRLLDAGALVEAPFGGCVGPERPGCARGSAVVSGRYSYTGLV
                     LSLLSDTTLRYWDLQSQVNYQLGPRDQLSLLAFGAYDLFKAENSGFDKVGGETRFFRG
                     DLRWDHEFSDRTRARLAVTGGLDSTGDDANVVRDRSVRGRLEVDHTFSEQASMRAAMD
                     VRFDDYALDANALESAYADFSALFPARTEVITGAYVTANLKPTKGFMIVPGLRTDVYR
                     AQGFTRVAVDPRISAVIELNRTLSFEHSFGVAHQPPNFIPSVPGAQVADLRRGLQRAL
                     MASSGVRVKLGTALSLQANVFRTAYFNALDPIGGSRDFSVDRAALERRATLSAAGLEI
                     KLSRPLTRRFGGFLAYTLSRSEQSSGALHTISGFDRPHVVQAALSYDFGAGIRAGVRG
                     VYYSGIPARLLGQETPRFTSDRRGAPYFRLDARLEKRWRLGRSGYIGVTAEVLNATYT
                     KEVLRLDCGGVCREDTAGPVILPSVGVEGGI"
BASE COUNT         1679 a         3235 c         3375 g         1816 t
ORIGIN      
        1 cgagcgtttc gcgcgccacc cgtgcgtact cgctatctcc ggtgagctga aatgcctcca
       61 aatacaccac cgtgagctgc gcattgtcgt acagcatctt ctcgaagtgc ggcacgagcc
      121 agcgctcgtc cgtggagtaa cgagcaaagc ctcccgcgag gtggtcgtaa atgccgccgt
      181 tcttcatgcc gtccaaggtg gtgcgcagca tggtcagcag cgttggatcc cggcttttgt
      241 cgtagagccg cagcagcaag cgcagcccct ggtgaggcgg gaatttcggg gctctcccga
      301 aacctccgta ctgcgcgtcg aagctctgcg agaagtcgcg caccacgcgc gtgaagctct
      361 cactggcgat cgccgcaggc gcggtgatgc cgagctcttg tcgcacgtgt tccgtcaact
      421 cggcggcttg cccgagcaag ctttcgcgtt ggctcttcca cagctcgtgg atgcgctgca
      481 ggaggttcaa gaagcccggg cgtccatagc ggtcgtcggg tggaaagtaa gttccagcga
      541 agaacggtcg cgtgtcgggc gacaaaaaca cggacatcgg ccagccgccg ctgccggaga
      601 gcgccacggt ggcggccatg tacacatcat ccagatcggg gcgctcctcg cgatcgacct
      661 tgatcgatac gaagtgctcg ttgagaaact gtgcgacccg agcgttctcg aacgactccc
      721 gctccatcac gtggcaccag tgacatgccg cgtagccgat actcagaaag ataggcttgt
      781 cttcgctgcg cgctttgctg agcgcggcct ctccccaagg aaaccaatct accgggttat
      841 gcgcgtgctg cagcagatac gggctggtct catgggctag gcggttcatg aaggccggaa
      901 tctagcacca cgaagctctc cctccgcgag cctccacgag cacggcgcct cggagtggcg
      961 ccgcagcgct ctcggtaggg gcgcacttgg gcagcgtgga gctcgcgcgt ccgggtttat
     1021 ccgagccaac ccagctggtg cgaatagcgc agcgccaaat agcccaaacc ggcgccgagc
     1081 acggctccca gtaacaggat caggagcact cgcgcggccg agcgcgggcg tccgggcacg
     1141 gcgggtacgc ccacgacgtt cggaacgtct ggaaccgcga acccaactgt cggcggaaca
     1201 ctgggtgacg ggcggggcga ttgcggagat tgcggggatt gcggagattg cggaggtgcg
     1261 ctgggccgcg ccgctccgcc cgcagcccac gggcccgagg ccagggttgc agcgccgggt
     1321 gagctcgaaa aatctcgcgg taacgcggcg gcccccggaa acgggctgtg cgccgcgggc
     1381 tcgggcaacc cgcccgctgc tccaccgaag cccacgaaac cgaaagccgc tgcatcctgt
     1441 gtcgaaccgt cgggcccaaa actttggctc accactctct gcatcccttg agttcgcggc
     1501 acgccccgcg cgctcgcggc ttcgggtggt ggcaccgacg gccgggcggg atagacgcgc
     1561 aaaagaaagc cgccttcagc caagggctct gccgctacgt gcaacccttg ggttgcgtag
     1621 tgctgctggt agcgggccac ttcatagcga tcttgttcgg gattgcccgt ccgaaccacg
     1681 aacccctgcg gtgcgttcat gtccaacaac ctttcatttc cccggtccgc ccgcacagcc
     1741 tacgtcatca cacctcgcgg cggtaggggc ttctgagtcg gcccgtatat aaaacattct
     1801 cttattgagc cttctcaccc cagaggcatc gactcatcga tcgagcctcg ctattctgcg
     1861 cgcttctgaa ggggaacgca catgctgcat ttcgatggga aggtcgtgct cgtgaccggt
     1921 ggaagttccg ggatcgggcg tgctgccgcg caagccttcg ccgatgccgg ggcgaaagta
     1981 atcctcacgg cacgaggccg ggagctcggg gaggagacgg cagctgccat tcgtcaacgc
     2041 ggcggcaatg ttagcttcgt ccgcgcggat gtttcgcgcg ccaccgacgt caagacagtg
     2101 ctgggtgaga tcgtcagccg tcatgggcgg ctcgacgcgg cgttcaataa cgcggcgagt
     2161 gatgtgggca tgtttcaact cacggctgac ttcaacgagg cgcagttcga cgagtgcatg
     2221 cagctcaatc tcaagagtat ctggttgtgc atgaaatacg agattgaaca gatgttgaag
     2281 caacgcccac gcggaggcgc gatcgtcaac acctcttcgg tcagcggtct cgggggttcc
     2341 ccgtcgggcg ccttgtattc cgcagcaaag gccggtgtgt tggcgctcac caaagccgcg
     2401 gcgcaggagt atgcgacctc tggcctgagg gtaaacgcat tggttgcggg tgcgtttcgg
     2461 actccaatgc tcaagcgcgt gttcacgcgg gcttccggcg gaaccccgga gggcatggcg
     2521 caagtggagg accgctacgt ctcgttgatc cctcagggtc gcatcggcgc tcccgaagag
     2581 gcagccaacg ccgccgtttg gttgtgctcg gatgccgcgt cctacgtgac cgggcattcg
     2641 atgatcgtgg acggagggat gacctccatg ctgcgctgaa gcccggtgtc gctagtgcct
     2701 caggtgtccc gggccagcaa caccaggtgc tctcgccggt cgcgctgggt aaaggcgcgg
     2761 ctcgagggtt cgtggaagtt cggtcgcgtt tgcgaagccc tcgcgatcaa acgcagcccg
     2821 gcgcggtcca cgagcggcgg cagccactca cccacgaaca acggccggcc cgagacggcg
     2881 ctatcggcaa tcaaaagcgc gacccgtcct tcgcgagtaa tcacgcgcgc aagctcgctc
     2941 aaacaatgcg tgaagtcgcg ctcgaagcct tgcaggccgc gttggctgtc ccgctcgaac
     3001 gctcgccgcg cgccgagctc accgcgccga aaatcctccc ccggcatgcg caaccagcgc
     3061 aacctcgcag agtggtgcgc gaagtagtcg tacaccccgg gatagggcgg cgagcttacg
     3121 accaaatcca cactgcccga cttcaggaat cccaactgtc gcgcatcgca ctgctccacg
     3181 cgcgcgggcg gcgttcccgg cggcatgagc tcggtgaagg tgcggagttg ctcgactagc
     3241 tcgcgagtcc gcaggaggaa gagcctgaat gcgaagctcc gcgccaagcg cttgggctgg
     3301 tccccgtcac tcgagtcccc gcgcttacgg ctgagcttgg tgagcatcga ggacaacacc
     3361 aggaacagcg tgcggcgggt ctcttcgggc gccgcacgtt caatgccgtc gcgtagccca
     3421 tccagggcca gcagcacgta ggtctcgtac agttggcgat cttccggacc gtaggggtgc
     3481 gtgggtccaa gcttcttggc ttggcgctct tttgcgtgtt ccacgacccg ctcggccgcc
     3541 gctaacagcg cttccgcgaa ccccggggga caaccggtgg ttttgactcg acacagcgcg
     3601 accgagagtg gattcaaatc gctgccgatg gctcgacgcc cgagctcgcg agcggagacg
     3661 accacggtcc cactgccggc gaacggatcc aaaacagtgg ccctgggtga gctcagtgtc
     3721 gcaatcaacg ccagggccgt gtcggggtgc atccgtgccg gatagctatg gaagccgtgg
     3781 acgtgcgctc tcacgcggtc ttcgtcgggg tcgacgtcca gcgcgcggcg cagcgcctct
     3841 ccgagctccg ggtcgcgcgc caccacgtcg acttcgccgc cgacatgagt gagggcgcgg
     3901 cgcgggcgta tggggggctc tcgggcggac aagcgggcgc atcatgaccg cgcccccgag
     3961 gtgtgcaaat cctatcgggg gataagcgca gctaaatagt cagctaacgt gaggaagaat
     4021 cggttaattg tgcgcccgcg cggacccggg ccgtgccgcc tggtatacac gcgggcgccc
     4081 atgctgctgg tcatcgatat cgggaacacc aatgtcgtct ttggtctcta cgacggagag
     4141 acgctgactc atacgtttcg catggccacc gtgcacaccc gcaccgaaga cgaatacggc
     4201 gcgttgctgc tgcagctctt gcagctgcgg gggctgcccg tcaattgcgt ggagtgcgcg
     4261 atcttggcca gtgtggtgcc gcctctcacc gacgtcatga tcgacgccgt gcgcagcgcg
     4321 tttgcgcgcg agccgctggt ggtggggccc ggtttgaaga ccgggatccc cgttctgtac
     4381 gaaaatccgc gcgatgtcgg cgcggaccga gtggtcaatg ccgtggccgc tttcgaacgc
     4441 tacaaggaag cgaccatcgt cgtggatttt gggaccgcga ccacgttcga ttgcatctca
     4501 ccgaaaggtg agtacttggg cggcgtgatc gtgcccggga tgcgtatcag cttggacgca
     4561 ctactcgatc gcgcggccaa gctctcgcgc atcgagctgt ctgcaccgcc ccgagtgctc
     4621 ggtcgcaaca cgacccatgc gctgcagtct gggttgatct tcggccatgc gtcgatggtg
     4681 gacggtatga tcgagcggct cgagcaagag gcgggttacc cgagtcgcgt catcgcgacg
     4741 ggtggcctca gtggcctagt gagcaagcac gctcgccgca tcgaagccgt cgacgtcaac
     4801 ctcacgctcg aggggctccg aattttacac gagcgtaacg tgcagggcgc ggattcgcgc
     4861 gccaacaacg tgaagcgctc acgcgcgccg aagaactcgt gatcgcgcgg cgcaggttcc
     4921 tttcagcggg cgtgggcgca ctgctcgcgt gcggttcgcg cggggcgggc tctgtcgcgc
     4981 tcgggtcggg catggcttgc agcaagcaac gggcgccttc gcgcgggcga actcgcatcg
     5041 tgtcgctggc gccggatcta acgcacacac tcttctcgat tggcgctggg gagctggtgg
     5101 tcggtgtgag cgactattgc caagaccccg aggccgtaca gcgcctgcct cgggtaggca
     5161 cgagcctcac gcccaactac gaagccatta cccggctgga gccgaccttg atcctgagcg
     5221 aagacaaccg aagcgcgcgc aagcgcgagc tctcggccat cgcgcgggcc gagttcttgc
     5281 catggctctc gctcgcggag gtggcagcca gtattcgcgt gctggggcga ctgacgcagc
     5341 acgggcgcga ggccaatcag ctcgcggagc gcttggtctc ggtgctcgga gtgccgccgc
     5401 cgacggcggg cccccgggtg ttactggtgt tgggagacgg cgcggacgca ccgggagagc
     5461 tgtggttcgt tcggcaaaac tcgctgcatg gagcggcctt gaacgcggcc ggcgcacgca
     5521 acgcggtgcc agaggcggta ttggccgcgc cacgtctgtc ttatgagcgg ctcgtccagc
     5581 tcgatcccga cgccattcta ttgctgtgcc tgcccaatcg agcggcggag cgcgccacca
     5641 ccatcggcac gttgcagcgc ttcgagaccc tctcggccgt caaacggcgg cgcgtgggca
     5701 tattggagag cgaggccgcg ttcgcgaacg gcccgcgcat ccttacgctc gtgccggcct
     5761 tgcgggatcg tttgaaggcg ctggagctcg tgccgtgacc gcggctctgc gcgtccaagg
     5821 cgtaggcgtg aaactcgcgg gtcgcctggt gctcgacgcg gtctcgctcg aactcgatcc
     5881 tggtcgcttg ctcgccctgg ttgggcccaa cggcgcgggt aagaccacgc tgcttcgagc
     5941 ggcactcggc gggctacctt ggtgccaggg cgaagtcttg ctgggcgggc gctctttgcg
     6001 cagttacacg ccgcgcgaac gcgctgcttg gcttggtttt ctgccccagc agttggcgag
     6061 cccggaaccg ttgccggtgc tcgaagtggt gcagaccggg cgcttccgat tcgacgagag
     6121 tgagtccgct tcacgccgcg ccgcgctctt ggccctggaa cgcgccggca tctcggccct
     6181 gcgagacgcc cgcctaactg agctttctgg aggtgaacgg cagcgggtgg caatggctac
     6241 gctgctcgcg caggaagcgc ggttggccct gctcgacgag cccgcgagcc acctggatcc
     6301 tgctcaacaa atcgattcat acggcttgat ttcagagctc ttggagcgcg gtctcggagt
     6361 gctcttggtc acccacgata tcaatttgct gtcccacatc gcgataccgg atcgggtgcg
     6421 tgtactcggc ctcgcgcgcg gtcgctcgat gttcgagctg gctttccaag atccggcgct
     6481 acccggcgcg ctcacccggc tgttcggggt tcgattccac gagttcgacg ggcagggcaa
     6541 gcgcctgctt ttggccctcg agcgcgtcga gggcgagtcg gagccgctga agtgagctcg
     6601 aaaccgcgtg ttttgctgct cagcgcggcc tcgctggcgg tgatggcggg ctcgctgttc
     6661 gtggggcccg cattcgacgg accgcacgcg agcttcgtgc tgctcgagct gcggttgccg
     6721 cgtctgctcg tggcgatgct ggtcggcgcg gccttggccc tgtctggcgc ggcgtttcag
     6781 atcttgttca ataacccgct cgccacgccg agcacgattg gcacgctctc gggagctacg
     6841 ttgggtgcgc tagcatcgat agttctcggg gtggacgctg aactctgggg gttgtcggcc
     6901 accacgctct gcgcgttcgt gggcgcgttg ctcacgagtt tgttcgcgct cggcgtggcc
     6961 gtcagcgggc gcgcgcggat gagcgatgta ttgctcgcgg gcatcgcgat tggtctttct
     7021 acgagcgcgc tcgcgaccgg gctcgagtac gcggccgaca gcaggatggt ctttgccgcg
     7081 agccgttggt cgctcgggca actgccgcag gttggttatc gcggtgtatg gctgctgctg
     7141 ccgatcgtgg tgctcacgac ggcggtgaca ctcagccaaa cacgtgctct cagcagcttc
     7201 atgctcgggg aagagatggc ccgcgcgcag ggggttcaca ttggccggct gcggagcatc
     7261 gtattggtga atgcgtcgtt ggccgtagcg gccgtcgtcg cttggtgcgg cccgatcgct
     7321 ttcgtgggct tgatcgtgcc gcacttggtg cggctcagtg tcggcgcgtc gctccgcttg
     7381 gtgctgccgt tgtccgtcgt ggtgggggcg ggctttctcg cctttgcgga cgggctctcg
     7441 cgtgctctcc tgcccgggcg tgaactgccg gtgggcgtgc tcacggcggc gctcggcgcg
     7501 ccagccttga tttggctttt ggcgcgccag cgacgctgag gaccgttccg ctgcaattcg
     7561 gctctggctc tgggtgctta ggacgacgat ccgtcgaagc gggtcacttc tcgaagatct
     7621 gagccgccgc taggcccgag cctgctgcga cttggatcac gactttgacc ggtgccgcga
     7681 gcgggaaggg ccacttgtag cagttcggtt tgcgaccaat caccgcggtc gggccagtgt
     7741 cgctgtccgt cgcgagtacc ggagacaggt tgggcagcgg cgtggtggcg acgagctgca
     7801 cgttcaactc ggtgacgggc gggagcgccg tggccacgat cgagtagcac ttctgtggct
     7861 gaagctggag ctgcgcttcg agcgtttgcc ccgtttggaa gctgccggcg accaccgagc
     7921 ctagcggctt gctacccgca acgatgtgct gcttggagag ctcgttaatc acgggctgta
     7981 cgaccgcggc ggcagcgggg tcgagttgct gggccttcgc gcccgtagtg ccgggcaacg
     8041 ctccgccgac gcctcccgcc ggcgcacccg cacctggaat tccgctcggt gcgcccccga
     8101 cgccaccaac gccggcggtg ccatacccgg gtggtacgtt ggcttgcccg cccacgcctc
     8161 cgtagccctg ggccggataa cctgcatagc cctgcccgcc gtaacctccc ggtgggttct
     8221 gctggttgta ttggacttgg ccctgctggt agccacccgt ctcggcgtct ttcttgccgc
     8281 acgcgctggc gaggccgatc agaacggcaa tcttgagcgc gctctcggcg gctcgctgcg
     8341 tggacggcat tcttccggcg gaaattcgga cagacaacat gcgaagctcc ttggcggcgt
     8401 gaaacccacg accgagatcc cccttactcc ggtcggcagg gccgaggcaa atcgaaaacc
     8461 cggcggctcg agcgccgact ggttcagatt ccgccttcga cacccacgct gggcagaatc
     8521 acgggccccg ccgtgtcctc tcggcaaacg ccgccgcaat ccagacgcaa gacctctttc
     8581 gtatacgtgg cgttcaagac ttcggcggtg actccgatat agccggagcg cccgagacgc
     8641 caacgtttct ccaggcgcgc atcgagccgg aaatatgggg cgccacgccg atcggacgtg
     8701 aagcgaggcg tctcttgccc gagcagacgc gcggggatgc ccgagtagta aacaccgcga
     8761 acgccagcgc gaatccccgc tccgaagtcg tagctgagcg cagcctgcac cacgtggggc
     8821 cggtcgaagc cggagatggt gtgcagcgcg ccgctcgatt gttcggaacg cgacagcgta
     8881 taggccaaga agcccccgaa gcggcgcgtg agcgggcggg agagcttgat ctcgaggcct
     8941 gccgccgaca acgtcgctcg gcgctccaga gccgcgcggt cgaccgaaaa atcgcgcgag
     9001 cctccgatcg gatcgagagc gttgaagtaa gcggtgcgga acacattggc ttgcagcgag
     9061 agcgcggtac cgagcttcac acgtactccg gagctcgcca tcaacgcgcg ttgtaggcca
     9121 cgccgaaggt cggccacttg agcgccgggc acgctcggta tgaagttcgg cggctgatgg
     9181 gcgacgccga acgaatgctc gaaggacagg gtccggttca gctcgatgac cgccgaaatg
     9241 cgcggatcca cggcgacgcg cgtgaagccc tgcgcgcggt acacgtcggt acgcagcccg
     9301 ggcacgatca tgaagccctt ggtgggcttc aaattggcgg tcacgtacgc gccggtgatg
     9361 acctccgtgc gcgcgggaaa cagcgccgag aaatccgcat aagcgctctc caaggcgttg
     9421 gcgtcgagcg cgtaatcgtc gaagcgcacg tccatcgcgg cccgcatgct ggcctgttcg
     9481 gagaacgtat gatccacctc gagacggccg cgtacggagc ggtcccgcac gacgttggcg
     9541 tcgtctccgg tgctgtctaa gccgcccgtt acggccaggc gtgcccgcgt gcgatcactg
     9601 aactcgtggt cccagcgcaa atcgccgcgg aagaagcgcg tctctccacc aactttgtcg
     9661 aagccgctgt tttcggcttt gaacaagtcg tatgccccaa aggcgagcag gctcagctgg
     9721 tcccgcggac caagctggta attgacctgc gactgcaagt cccaatatct cagcgtcgtg
     9781 tcgctgagca gcgatagcac cagccccgta tacgaataac gaccgctcac tacggcgctg
     9841 ccgcgcgcac aaccgggccg ttcggggcca acacagccgc cgaacggcgc ctccaccagc
     9901 gctccggcat ccaacaagcg gatgctcgct tcaccccgca actggtgcga gagcgggcgt
     9961 gtgcgtgccg cgacgatcgg acctgcgaag cgtcccaagt tgaccggcgc ggcgcccgca
    10021 tagagctcga tatcttcgat catccccgga tgcaggaccg agggaccgaa gaaggcgtga
    10081 tacaagagcg ggacatcgac gccgt
//
LOCUS       DASTKU010001058         3590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_878540, whole genome shotgun sequence.
ACCESSION   DASTKU010001058 DASTKU010000000
VERSION     DASTKU010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878540"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFQ61_37610"
     CDS             <1..400
                     /locus_tag="VFQ61_37610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960287.1"
                     /translation="TWHVWEEAYRVEVVRPGNSQVRWTTTLEGVMRRCAEARRLLAAD
                     RSQLPELSALRVTGKIQVNPVSQEVLHKIQRWVMRPASTGTAAPGDALFSTFTGLFLQ
                     RIGDAEREIKFVTRPRMPSIFHSRDEQQVQ"
     gene            500..1099
                     /locus_tag="VFQ61_37615"
     CDS             500..1099
                     /locus_tag="VFQ61_37615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9960288.1"
                     /translation="MARSFPLALQLAGKPCLLLGSSAETHERALALLEAGALLHVISE
                     QPSTELRALATQHGFCLECRRFDAADLDGKWLAVSCDRDAELSARLAQEAAARRIFHC
                     AIDQPEGSTFSHMARVRAGLVSLCISTEGRAPALGRRLRQELSRVIEASRLAEFAHEV
                     AELRDRLKPSERKAQLESVVQGLRFEGQLQVPPAVTHEP"
     gene            complement(1145..1834)
                     /locus_tag="VFQ61_37620"
     CDS             complement(1145..1834)
                     /locus_tag="VFQ61_37620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960289.1"
                     /translation="MPGPPSTRTSFPSASGEPSLRRLGLGSASGAERPVRAQIVVLVV
                     LALTIVAVPMYLVRRPNATKRAEPSTSPSASAALVGSARPPEKIEASKPKLPERVRLG
                     AAQRVRCGATPKATQEGNLCDGLPVFEDALAKSIVDENCVAQVKAAGTINFVLNVDFA
                     RRALHVFPGASGEFRGPQARRVTNCVKRALPKPDWEAIRHQYKYYTIAVLATFTPDAG
                     GTASGGPPKFD"
     gene            complement(1858..3504)
                     /locus_tag="VFQ61_37625"
     CDS             complement(1858..3504)
                     /locus_tag="VFQ61_37625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HET9960290.1"
                     /translation="MNDRYEIRQSLGKGGMGEVFLAHDRSTQQLVALKVVREESRMPG
                     DDEALRQELLLARSVGHPNVCRVHDLAPSPWGPILVMEHIAGQTLHTHIRRRKAQGGY
                     TADEFRKIASEAAAGLAAIHAQGLVHGDLKPGNVMVTNDRAIILDFGFAQERARLSAR
                     RPGAPPDGGTPNYMAPERLRSGGASPEDDIYALGLTLWEMWTCRVPEPGYRPRAKPMR
                     SQIMFDVPAGLSVDEVKQVFRCLSDDPAMRPLGRHLRFFNPSSLTTSQVQIPRERISP
                     GAPPGRATAETFVPGAQSLLVTYCTNAPEAAGTLYELKKPVITIGRRGGQDIMMPEAT
                     VSGSHAVLKWQSGSWTIEDAGSTNGTYADYGYERKKTIALLHGGEVQLGESRVKLVSF
                     SEGSPQHERAKAYLARRDGLTGLLRREHLVRAVEEDVAFADWSNNPLSVARYYIRSPN
                     RHISDRPTILEMLALRRAAQRVVELTEVLLLSIVPVTGGRTGPLKFAIVMTGPSPEEA
                     RNVVEQVVSQVQGLMPDTLDLGATLARAEPGQRVTQLLDE"
BASE COUNT          619 a         1219 c         1142 g          610 t
ORIGIN      
        1 cacctggcac gtctgggagg aggcttaccg cgtggaagtg gtgaggccgg gcaatagcca
       61 agtacgctgg acgactactc tggagggtgt gatgcggcgc tgcgcggaag cgcggcgctt
      121 gctcgccgcc gatcgcagtc aactcccaga gctctcggcg ctgcgggtca cgggcaagat
      181 tcaggtcaac cccgtgagcc aagaggttct gcacaagatc cagcgctggg tcatgcgccc
      241 ggcgagcacc gggacggccg cccccgggga cgctttgttc agcaccttca ccggcttgtt
      301 tctgcagcgc atcggtgacg cggagcgaga aatcaagttc gtgacccgcc cccgcatgcc
      361 aagcatcttt cactctcgag acgagcagca ggtgcagtga cgcacggctc ggcgagcgcc
      421 tgcgaaccga gccctcgaac ttgaacaggg tccagcggcg ccgattggag aatgccgcgc
      481 gcagaactat gctgaagggg tggctcgatc gtttccgttg gcgctccagt tggccggaaa
      541 accgtgtctc ctcctcggct cctccgcgga gacccatgaa cgcgcgctgg cgctgctgga
      601 agcgggcgcg ctcttgcacg tcatttccga acagccgagc accgagcttc gcgcgctcgc
      661 cacccagcac ggcttctgcc tcgagtgtcg ccgcttcgac gccgccgacc tggacggcaa
      721 gtggctcgcc gtatcctgcg atcgagatgc cgagctctcg gcccgcttgg cgcaggaagc
      781 cgcggctcgc cgcatctttc actgcgccat cgatcagccc gagggcagca ccttttcgca
      841 catggctcgc gtgcgcgcgg gcctcgtcag tctgtgcatc agcaccgaag ggcgggcgcc
      901 cgcactcggt cgtcggctca ggcaagagct ctcgcgcgtg atcgaagcct cacgcctggc
      961 tgagttcgcg cacgaagtcg ccgagctgcg cgaccgtctc aagccgagcg agcgcaaggc
     1021 gcagctcgag agtgtggtgc aagggttgcg cttcgagggc cagctgcaag tgccgcccgc
     1081 cgtaacccac gagccatgag ccatgagctc tcttggcggg aagcccccct atcccgatcg
     1141 gcaatcaatc gaacttcggt ggcccaccgc tcgcggtgcc ccccgcatcg ggcgtgaacg
     1201 tcgcgagcac ggcaatcgtg tagtacttgt actggtgacg aatcgcctcc caatcgggct
     1261 tcggcaaagc gcgcttcacg cagttggtca cgcgccgcgc ctgcggcccc cggaactcgc
     1321 cgctcgcgcc ggggaacacg tgcaaggctc gacgagcaaa gtccacgttc agcacgaagt
     1381 tgatggtacc cgcggctttg acctgggcca cgcagttctc gtcgacgatg ctcttggcca
     1441 aagcatcctc gaacacgggt aagccatcac acaggttccc ttcctgagtg gctttgggag
     1501 tcgcgccgca gcgaaccctc tgggcggcgc ccaagcgcac ccgttcgggc agcttgggct
     1561 tgcttgcttc gatcttctcc ggcggtcgcg ccgaaccaac cagggcggcg ctcgcggacg
     1621 gactcgtcga gggttcggct cgcttggtgg cgttcggacg gcgcaccagg tacatcggca
     1681 ccgccacgat cgtgagcgcc agcacgacta acaccacgat ttgcgcccga accgggcgct
     1741 ccgcgccgct cgcgctgccc agacccaaac gacgaagcga cggctcgccc gacgccgagg
     1801 gaaacgatgt tcgcgtggag ggcggtcccg gcatgcgctc agttcatccc tgcagcttca
     1861 ttcatcgagc agctgagtga cgcgctggcc cggctccgcg cgcgccaaag tagcgcccaa
     1921 atccaaggtg tccggcatca agccctgcac ctgtgacacg acttgttcca cgacgttgcg
     1981 agcctcttcg gggctcggcc cggtcatcac gatcgcgaac ttgagcgggc ccgtacgccc
     2041 gcccgtgacc ggcacgatgc tgagcagcag cacctcggtc aactccacca cgcgctgggc
     2101 ggcgcgacga agcgccagca tctccaggat ggtggggcga tcgctgatat gccgattcgg
     2161 gcttcgaatg tagtaacgcg ccaccgacag cgggttgttc gaccaatccg cgaaggcgac
     2221 gtcttcttcc acggcgcgca ccagatgctc gcggcgcagc agaccggtga ggccgtcccg
     2281 ccgagccagg taagccttgg cccgctcgtg ttggggtgag ccttcgctga agctcacgag
     2341 cttgacgcgc gactcaccga gctgcacctc gccgccgtgc agcagcgcaa tggtcttctt
     2401 ccgctcgtat ccgtagtccg cgtaggtgcc gttcgtgctg cccgcgtcct cgatggtcca
     2461 agatcccgac tgccacttga gaacggcatg agagccactg accgtggcct cgggcatcat
     2521 gatgtcctgc ccgccccgac gcccaatcgt aatcacgggc ttcttgagct cgtacaaagt
     2581 gccggcagcc tctggcgcgt tcgtgcaata ggtcacgagt aaggactgag ctccgggcac
     2641 gaacgtttcc gcagtggcac gccccggcgg ggcccccggt gagatccgct cgcgaggaat
     2701 ctgcacctga ctggtggtca aagagctcgg attgaagaag cgcaaatgac ggccgagcgg
     2761 gcgcatcgcc gggtcatcac tcaggcagcg gaacacctgc ttgacctcat cgacggacag
     2821 ccccgcaggc acgtcgaaca tgatctgcga gcgcatgggc ttcgcgcgtg gacggtagcc
     2881 gggctccggc acgcgacacg tccacatttc ccaaagcgtc agacccaagg cgtagatgtc
     2941 gtcctcgggg cttgctccac cagagcgcag ccgttcgggc gccatgtagt tcggcgttcc
     3001 gccgtcgggt ggcgcaccgg gacggcgcgc gctcaagcgc gcgcgttcct gagcgaaacc
     3061 aaagtcgaga atgatggcgc gatcgttggt caccatcacg ttgcccggct tcaagtcgcc
     3121 gtgcaccaag ccttgcgcgt gaatggctgc gagccccgcc gcggcctcgc tcgcgatctt
     3181 ccggaactcg tccgccgtgt aaccgccctg cgctttacgc cgtcgaatgt gggtatgcag
     3241 cgtctgcccg gcgatgtgct ccatcaccag aatgggcccc caagggctcg gcgccaaatc
     3301 gtgcacgcgg cacacgttgg ggtgaccgac ggagcgcgcg agcagcagct cctgacgaag
     3361 cgcttcgtca tccccgggca tgcgcgattc ttcccgaacg actttcagcg caacgagctg
     3421 ctgggtactg cggtcatgag cgaggaaaac ctcacccatt ccgcctttgc ctaagctctg
     3481 ccgaatctcg taccggtcat tcactaagga accggggcct agcggcggga catttcgggt
     3541 catttcgcag cgaccggggc gttctcagga cgcacagggg gctagcgtcg
//
LOCUS       DASTKU010001059        23471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1772859, whole genome shotgun sequence.
ACCESSION   DASTKU010001059 DASTKU010000000
VERSION     DASTKU010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 23471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23471
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772859"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VFQ61_37630"
     CDS             <1..974
                     /locus_tag="VFQ61_37630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960291.1"
                     /translation="LESQTGRRCVLIAESDNNDPRMTRPRAEHGLGMTAQWNEEFHRS
                     LHTALTPERAGWYADYHGLEDLLCVLRHGFAHRGRYSTFRKRRHGRALSEVPHRRCVA
                     YLQTHDLVGNRPAGERLGQLVEPRAARLAAALLLLGPWVPMLFQGEEWNASSPFLYFT
                     DHGDPGLGRAVLAGRKRDFAGFGWTDTRTPDPQSESTYLRSRLQWSELDDAAHAAFLS
                     SYRRLIELRRSWAELTAPARAELDGNCLQLWREQSIAFFNFSSEVYARSLFELDEPGE
                     LGPLEIVLASNVEGEANSGGHAVGTVIESGEIRLGPFAFLLVRRSRP"
     gene            1262..2461
                     /locus_tag="VFQ61_37635"
     CDS             1262..2461
                     /locus_tag="VFQ61_37635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HET9960292.1"
                     /translation="MTQHSVLIVGGGTAGISVAARLRRHDPALEITLIEPSEFHYYQP
                     LWSLVGAGVFAKERTRRPQSDCIPPGVEWLKTRVVRFEPAEHALLTADGQWHSYEQLI
                     VAVGIQLDWHKIEGLPPISSGVMGADTGICSNYSYETVDSTWQALQAFSEGNALFTLP
                     ATPVKCAGAPQKIMYLTDDYLRLRGMRERARIWYAASSASIFGVEKYARTLRQVVERK
                     HIQTLFRHHLVRVDVAERKATFQHLDSGEQRTLDYNLLHVTPPQSAPDVIKQSPLANP
                     DGWVEVDKHSLQHKRFPDVFALGDASSLPTSRTGAAVRRQAPVLVENLLAHRARQRLQ
                     ASYSGYSSCPLITGYGRLVLAEFDYDGQPMESFPIDQARERYSMYLFKAYALPALYWR
                     AILRGRL"
     gene            2504..3148
                     /locus_tag="VFQ61_37640"
     CDS             2504..3148
                     /locus_tag="VFQ61_37640"
                     /inference="COORDINATES: protein motif:HMM:NF012479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET9960293.1"
                     /translation="MKSPSVAVLLGSLLVTWLPGCDRSKPEASVAPAPAAAPSAVLSP
                     AVSSSAAQTNVSPYTLQVKTLEGAALPLEQFRGSVTLFVNVASECGYTPQYAGLQRIY
                     EKYRARGFSVLGFPSNDFGRQEPGSPAEIRAFCSSKYRVNFPMFAKVSTRSGPDQSPV
                     YASLSAATGQLPSWNFGKYLVGRDGKVLHFYDSKVEPESAELTAAIEGALAAAR"
     gene            complement(3152..4006)
                     /locus_tag="VFQ61_37645"
     CDS             complement(3152..4006)
                     /locus_tag="VFQ61_37645"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HET9960294.1"
                     /translation="MSNSLLFSAALVAAACGLTSCGSPPLVTWTEGGNGSTAPGECAT
                     NPHPECVKPGETPNGPPDVVCKAGRPILTPEIVNARDLGGTPIVARPAEEAAEGAAEP
                     QSVACGAVFRGPPLVLSSAACGAFAELGIRTVIDLRVESERVAKPDSECVGATASIQL
                     SPLPVPYNVSTQDYIADLNTTESIAAVFHTLGSPDAYPVYLHCTWGRDRTGIVAALVL
                     LALGVAREDVMAEYSLSLPLVGAYPDSLAGMLDELEARGGIGAYLAGIGISDDELAVL
                     REHALPPL"
     gene            complement(4414..4593)
                     /locus_tag="VFQ61_37650"
     CDS             complement(4414..4593)
                     /locus_tag="VFQ61_37650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960295.1"
                     /translation="MHHRHGAWLGVSSISKTRHELETRHGSRFTCLEARAVGALVYLI
                     LARELGKAAQWRLER"
     gene            4605..6074
                     /locus_tag="VFQ61_37655"
     CDS             4605..6074
                     /locus_tag="VFQ61_37655"
                     /inference="COORDINATES: protein motif:HMM:NF013504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 6 protein"
                     /protein_id="HET9960296.1"
                     /translation="MRIASSRRVWISTLALIFVAACSSNQDANSDQEVLGVVSAEVQS
                     ADNLITATLKLETWAGGYNGTVTITNNGSTPTSSWLVRIDPGGSSVTNLWSAVAPARP
                     ITATFDVKNESYNGSIPAKGSVQFGFSGSGTGTPQILSVSQVGASGGSGSGGGGSGGS
                     SSGGSSSGGATARGGSSSGGTVGAGGSTSRGGSASGGSASGGSGAVSNTGNPFAGASL
                     YVETNSLCAQNAKSDSRLNTICQNPQGDWVGNWSGDPASAVRTLLSAASGKLRTLVAY
                     NIYRRDCGGASSGGAASPAAYRTWIDGFASGIGNSPVAVILEPDALAFNCNNTGNNDS
                     TLAMLKYAVEKIEAQPNAAVYIDAGHPNWISVSETVNRLKQAGIATATGFSLNVSNFY
                     STDSNISHGRSVAAGVGNKPFVIDTSRNGLTSSGSEWCNPRPRGLGKRPTGNTGQSGV
                     HAFLWVKRPGESDGACNGGPAAGQWYNDYALHLIQNAQL"
     gene            complement(6143..6346)
                     /locus_tag="VFQ61_37660"
     CDS             complement(6143..6346)
                     /locus_tag="VFQ61_37660"
                     /inference="COORDINATES: protein motif:HMM:NF013361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpyruvate tautomerase MIF-related protein"
                     /protein_id="HET9960297.1"
                     /translation="MTFAGSEEPACYAEIKNIGALPAKLTAELSRVLCEKLTHGLKVP
                     SNRIYLEFTENTPHLFGFDGETF"
     gene            complement(6343..6492)
                     /locus_tag="VFQ61_37665"
     CDS             complement(6343..6492)
                     /locus_tag="VFQ61_37665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960298.1"
                     /translation="MPVIRIVSSAPHPAPSARSEFLRELSTTLARLLNKPEAYVMTCL
                     EPQRT"
     gene            complement(6503..7639)
                     /locus_tag="VFQ61_37670"
     CDS             complement(6503..7639)
                     /locus_tag="VFQ61_37670"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET9960299.1"
                     /translation="MNLPPQKPMLRVARVLALAGAAAALGWTFRDVSVSHVLDLCARV
                     GAGGLGLVLLPQALSFAVDTWGWMRVARWLRYRVRYSSLLFVRVATESLALCLPAGVV
                     FAESAKIPMLMRHSGVQGAQAVALVSARKYLTLTAQGVYSIVVSALAAHALGFRWASF
                     PGPSSWALGVFAAACALLLLAFGTRSILVHANVTVRLGAAWDRVRQLPSVLHRRAPAH
                     GTAVRVSACSRRARFENTDAELARLFTFSAGREAWLTMGFVFGWLGESVETYVGLRLL
                     GVNLDFGAIAAMEVMLSLLRSLVVVLPAGIGVQDAGYALLLRALGVNDSVDTVAAFAL
                     LKRAKELLWVGVGLALLTRRPTIVTSTNGLSEPRFVLSTLRGDS"
     gene            complement(7738..8208)
                     /locus_tag="VFQ61_37675"
     CDS             complement(7738..8208)
                     /locus_tag="VFQ61_37675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960300.1"
                     /translation="MTTTPSSTSPGAMLEAIARADQLNLNPGMPGIDPYGDSLRAIRE
                     APGVEDELERIATEGTPTGRVWAVMLLRDLAPERATPHLRRMLTDDSPIEVNTCLVGF
                     RRTCDWARNQMAPRVRAAGRPSRPPSFIGRYALFLILGGCIAFMWIAHVALHWF"
     gene            complement(8205..8609)
                     /locus_tag="VFQ61_37680"
     CDS             complement(8205..8609)
                     /locus_tag="VFQ61_37680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960301.1"
                     /translation="MSLVEKDLIRRLIQDLAKFFARLAGLRAAGELDQAERELSEMMR
                     QALGPWSRDAEYLAVESLMKMLSRERLELYARLLIERGALAEARGQPERARRDQERAR
                     SLLDALARAGGATEAPNGVGLGPGTAGGKLDR"
     gene            8933..10603
                     /locus_tag="VFQ61_37685"
     CDS             8933..10603
                     /locus_tag="VFQ61_37685"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET9960302.1"
                     /translation="MRANSVVGWVRGDIHGQTPGRGTRLARGATRGIRWALAAAAALA
                     GPLCASGAQAEDLSYSGVDRATVRVLALGPAKVVPVEHRGVVYQIGVPLGGHGSGVLV
                     KENGLVLTAAHVVENARLLAVHLPGKREPVPAKVLYVNDAEDYAFIRIAGTYSEIAHF
                     AAPDEQLKVRSTIFAIGYPLDATRFDPQSSKGSISGKRPDGRLQLDIQVNPGNSGGPV
                     IDEKEQVQAIVVARSDVEKGQVGLAAAVPVSSFRSKYEELAQATPDTSDIDTEAAQRV
                     ANMVSTLAQDGLEWFKGSLESSESQNDINSRVRKQAEDMPASSDAQIMAAVYFWNRYM
                     VRLARGQEGQELHSRATQYVERAVKLEPGLKESSDFVEFVLGEGREERAPQHIGPRPR
                     HHEGFFLRAGYGLGYLSSSLEQNGGESLSGLLKQWEVLIGGGGRGMFWGAAFSNASGD
                     VDYEHKDGNGNTLKSTTHASVFRFGPFWQYFPSVTGGLNFRLQASIMGADASGASLNG
                     GFAASAGAGYDLWFSSAFNAGLNVGIAYLSGSQNSGWLPTATLSLGLR"
     gene            10737..12026
                     /locus_tag="VFQ61_37690"
     CDS             10737..12026
                     /locus_tag="VFQ61_37690"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET9960303.1"
                     /translation="MQFLLLVAFCAISALTLGLAIVNRRYQERHAHVVPPELQALSVA
                     QGQALFDADALRKAASYARDRSRFGVLRSIASRCMLLAFFFGGGLGVYDRWVSGLGLS
                     FIAQGVVFALGIMALSAVWELPFSVYRTFALEQKHGFNRMTPGLFISDALKGLVLGGL
                     MIAGLSAAALWLVRLLPNGYWLAIWGLLLAFNVFVTLLAPYVIEPLFFKMTELAIPEL
                     GERIQRLADRAGVRVSRVLKMDASRRSSHSNAYFTGFGPVKRVVLFDTLIERMMPDEI
                     LAVLAHELGHWKKRHITQRFLLGELASFVGLWIVSLLVAWPALPEWVFLNEASLPARA
                     VIVALAWSVFSFPLTPLASAWSRRHEREADRFARDLTQTPRALATALAKLARDNLASL
                     TYHPWFEAVYASHPSVVERVRTLTSASLHAAGETSAA"
     gene            complement(11996..18709)
                     /locus_tag="VFQ61_37695"
     CDS             complement(11996..18709)
                     /locus_tag="VFQ61_37695"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9960304.1"
                     /translation="MGENAVSQEFEDRNEGYQIAIVGMSGRFPGAPSPAALWENLKRG
                     LESVHFFSDEELLRNGESPENLADPNYVRAWPVLENMDHFDAGFFGMSPRDAAVMDPQ
                     HRLFLMVAWEALERAGYEPERTLGPVGVFAACGMNHYMMYHLVTNQEVMQTVGEWLVR
                     HNGNDMNFLATRTSYQMNLKGPSLNVQTACSSALTAVHLACQSLLAGECDYALAGAST
                     LSLPQDRGYVYKPGEILSPDGHCRPFDAGSHGTLFGSGTGCVVLRRLADAERDGDTIL
                     AVIRGSAINNDGAQKVGYLAPSVEGQARAISEALAISGVHAETIGYVETHGTGTAVGD
                     PIEVRALNEAFAPHTSRRGFCAIGSLKANIGHLGEAAGIAGLIKTVLSLRHGQVVPSI
                     NYSQPNPEIDFARSPFRVNTALADFPIPTPGFPARAGITALGAGGTNVHLILEAAPVS
                     QAKAGEAGPELVVLSARSDAALLQAKSALADHLNGHEAAPLADVAYTLAQGRKAFNKR
                     LAFVARDRADAVAALLSPDPKRIQSGTARSGKNEVAFMFPGGGAQYAGMGAALYSHEP
                     VYRKAVDECLSALDSEHRKKVSALLLERVNPQQASQELERPSLALPALFATEYAMARL
                     LMSLGLEPVSLIGHSMGEYVAASLAEVFSAADGMGLVTRRGRLFETLPEGTMLSVQLP
                     EQELRRHLSPELSIAAENAPELSVASGPVAAIQRLEQILNERGVETQRIHISVAAHSS
                     MLEPILREFESYCRSIRFHTPKRPFISNLTGSWVDPEQVKTPGYWVAHLRSTVRFNQG
                     ISTLLAEGNPLLLEVGPGRTLASLARQQPNVPFATPTMRHPRESAEDREFLLESLGRL
                     WVAGGELDFSALMSGARRRVPLPTYPFELKRYFIPANANASAQAAKVFRPHKQAQLES
                     WFGKCVFRELAEAGPTVPARRRLFFTDKLGLHRRLEAGSPENIHVSPGERFLKLSADR
                     YMLRVDAREDYAALFEDLEATQRLPDQIVHLFGVTPNHNRLGLSTRSAALAPEAYEGH
                     EAHYFKSLLYLSQEVAKRELELRLDVVTSQLHKVGNDPAPHPEKALVLGAVRVLPREL
                     PSVRTRCIDVSCDRGSDAELDRLAQELADELSRAPAPTHAADPIVALRGGMRFVQEVI
                     PAKPVESSSEASISGAPVRVRPRGVYLITGGFGGLGLTFAKHLALQAHARLVLVGRTV
                     LPSPAERARYLAEHTVDDPIARKLRAIQELEAAGADVMLAHVDAADFEQMSGLRERIR
                     ERFGKLDGILHAAGTLDDTLISLKTEDSARRVLASKVKSTLVLDRVFGRDRLDFVVLF
                     SSVSSVLGLEGQIDYTAANAFLDAYANAQLGPGRPLTISIGWNAWQEVGMAVEVTEAA
                     RPGGVTEHGRIGPHPCLERIAVDTPEEMLFVTRFTRVRHWPLGEHVVRGGAALLPGTG
                     YLELSRAGLSHALRTRALELENVAFLSPFTVGEGESRDLRLRLTLGAGDQVRFTFFSE
                     SEREPHATGSARPLSKQERPAVDLAALRERLRGPDQQIGGFLPQPFMDFGPRWANVES
                     VRLSSSDGAREALIELKLAALFSADLASYELHPALLDMATGGAQALVPGFDGGRDFLV
                     PLSYGKVRLVAPLTSHVFSHVRLRTGNTPALVSFDVTIHGAQGEVLVEIENFCMTRVD
                     QGRFAALPASGTGVTSATLPTERSAGLTRAPRAHESPRSQLSQVVAHGILPSEGVQVF
                     ERLLKQPRSGHWIASSVPIAEWLRSVDAKTLEARAASQHSSRPADPSAALNEDGETSR
                     AVHANYVAPRNELELELSELFSELLGVQHVGIHDDFFELGGQSLVAVRLFNKIRRKHA
                     VDLPLSTLFEAPTVAQCAEVIASELGITPAPLAIAANANAGPESTSASGPATMPSSPP
                     PAPGAEDETPPPSLRFRASDSAPPPPAGPNGVGSGDGGDAASGDAAHSSGQASHVRRT
                     RWRSLVPMQPHGELPPIFCVAGMGGTLNNLRKLALLAGDQRPFYGLQPPGADGQTEIL
                     YTVEALAEHYISEISSVCPDGPYLLGGYSGGGVAAFEIAKQLEARGKRVAFVGLIDSF
                     SPHLPTRPFVQRARMHAKRVIDRPSYLVETLGRRLTYESWNLGRVMARNLGKVFPERY
                     RYEQLADSWVLAEQRYRPTPWNGQVHLFRAREESALTLWTALEVDERYGWSRYALGGV
                     HVELCPGNHSTMCEEPNVRVLAAKLKEAVNRASPAPATYAAEVSPAA"
     gene            complement(18691..19701)
                     /locus_tag="VFQ61_37700"
     CDS             complement(18691..19701)
                     /locus_tag="VFQ61_37700"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET9960305.1"
                     /translation="MSEPLESTARGVSERSETSGDPGDPGGESGRRRGKNARRARPYR
                     RFAMAALLALLGAPALFVAWRSYRVEREMCFPKPTLAGSPADVGLADAREVSFESNGE
                     RLYGWYLPARNGGAIALFHGAGGNRASLAAEARALHEGGFGVLLLELPGHGRSEGDVR
                     WGRPEETAIRDAVQFLLRQPGVDAERLGALGFSLGGYMLARVARSDVRIRAVALEGTP
                     AHLGDRIRHTHRRWGTFGAACAKLAFLQSGHDPERDSAHAAVPEIAPRALLIVTSEQD
                     ELVPRAMAETMFAAARQPKALLVVPGNGHGGYASRSRSYAADLVEFFRQGLGLTPWER
                     TQ"
     gene            20043..20855
                     /locus_tag="VFQ61_37705"
     CDS             20043..20855
                     /locus_tag="VFQ61_37705"
                     /inference="COORDINATES: protein motif:HMM:NF045039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960306.1"
                     /translation="MSMTSALHCSVSLPGAALRSLSLVLGLAAVTACSSDSNSGTNTP
                     ASSCIFCDSFESYAAGPVAETPAFTPTTSGGTLTYDAVHARDKQALHVHTDGNGRAYI
                     ALKNFTPPNNSFWGRMYLWVDAFPTAPNYAHYTLVEAAGTPAGFVRPIGGQYIMEEGK
                     NMWGPGSDGGKTGDWTNWQTSAPAESGKWLCMEWQMAAADNSIKIWIDNVEKTDLAVS
                     TQNHGGTQFDFEFPVFDKLWLGWWLYQAGPTPDHFDIWMDDVAIGTERQSCK"
     gene            complement(21002..22117)
                     /locus_tag="VFQ61_37710"
     CDS             complement(21002..22117)
                     /locus_tag="VFQ61_37710"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9960307.1"
                     /translation="MTISDYYTLGRSGLRVSRLSLGVMTFGTEWGWGSAKPEAQRVFD
                     VYVEAGGNFIDTADAYTNGTSELWLGEFVRARSLRDQLVIASKFTFNLGERNANIGGN
                     GRKNILRAIEGSLRRLNTDYLDLYYLHAWDQVTPSEEVMRTLDDLVRSGKVRHIGLSD
                     VPAWYAARAQTLAEWRGFEPVSALQLQYSLVERNLEHEYTRLATELGMGIVAWSPLAS
                     GLLSGKYRASDAKQAKTEGRLAQMQGSGNPAFQHLTDRNFEIVAELDRVASELGRPMN
                     QVALNWITRRPGVASVILGASKPEQLESNLRALEFEIPAELSARLEEKSRPPTPFPYY
                     FFTNAMQGMMYGGATVGDKPPGYRAPVLVSGSGSGVT"
     gene            22215..23120
                     /locus_tag="VFQ61_37715"
     CDS             22215..23120
                     /locus_tag="VFQ61_37715"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9960308.1"
                     /translation="MKLDQLDGVLAFVTVAQKRSFSAAAAELEVTPPAISQAIKQLED
                     RLGVRLFNRTTRSVSLTEAGACFHAKVAPALQQIGEAAQGLDDFRSAPSGLLRLNSSH
                     VAWEMLLREAVRSFLEQQPDIRIEVHLEDAFVDIVEGGYDAGLRLGDSVQRDMVSTLL
                     TRHERFALVASPDYISRRGAPAQLNELRDHACIRFRFRGAGGIYRWELIDRGELLEIE
                     VQGPLTVSDSRSLREAALDGVGIAHTFLRLVEPDLAQGRLVAILPEHWPTFAGFSLYY
                     PHRAQQPAKLRAFIDHCRTLTAPHR"
BASE COUNT         4161 a         7884 c         7328 g         4098 t
ORIGIN      
        1 cgctcgaatc gcagacgggt agacgctgcg tgctgatcgc agagagcgac aacaacgacc
       61 cgcggatgac gcgcccgaga gccgagcacg gcctgggcat gaccgcgcag tggaacgagg
      121 aattccatcg ctccctgcac accgcgctga cgccggagcg cgcggggtgg tacgcggatt
      181 atcacggcct cgaggacctg ctgtgcgtct tgcgccacgg ctttgctcac cgcggtcgat
      241 acagcacctt tcgcaaaagg cgtcacggaa gggcgctctc cgaggtcccg catcggcgct
      301 gcgtggccta tctgcaaacc cacgatctgg tgggcaatcg cccggcaggt gagcgtttgg
      361 gccagctcgt cgagccccga gcggcacgct tggccgcggc gctactgtta ctggggccct
      421 gggtgccgat gttgttccaa ggcgaggaat ggaacgccag cagccccttc ctgtacttca
      481 cggatcatgg tgacccgggg ctggggcgcg ccgtgctagc tggcagaaag cgggactttg
      541 cgggctttgg ttggactgat acgcggactc ccgacccgca gagcgagagt acttacctgc
      601 gctcgcgact gcaatggtcg gagctcgacg acgcagcgca cgcggcgttt ctgtcctcgt
      661 accggcgctt gatcgagctg cgtcggagtt gggccgaact cacggccccg gcgcgcgccg
      721 agctcgatgg aaactgcctc caactctggc gtgagcagtc catcgccttc ttcaacttct
      781 cgagcgaagt ctacgcgcgc tccctgtttg aactggacga accgggcgaa ctgggcccgc
      841 tcgagatcgt ccttgccagc aacgtcgagg gcgaagcgaa ctcgggaggc catgccgtgg
      901 gaaccgtgat tgagtccggc gaaatccgcc tcgggccgtt cgccttcctg ctcgtgcggc
      961 ggtcgcgccc atgagcgccg cgcaatgtgg cgccgcgcgc gatacgatct aggccagcag
     1021 cttcgaggcc caatccacga actgtgacat cgattctcga cggccgagcg gttccgttgc
     1081 ttcggggcga cgcccgtcat ctcggctcct ggcaagcctc ctatccgcgc gccaaaaagc
     1141 aggccgagac ggatacagaa gcgtgcgtct gctagtcacg ggccgtgact ctgcgtaacg
     1201 gtaatgagcc ggcgctcggc cgtgcacgat cgcggggttt gcttgtcttg gagacgccag
     1261 cgtgactcag cattccgttc tcatcgtcgg tggcggcacg gccggaatca gcgtcgcggc
     1321 acgcctgcga cgtcacgacc ccgctctcga aatcacgctg atcgagccgt ctgagtttca
     1381 ctactaccag ccgctctggt cgctcgtggg cgcgggcgta ttcgccaaag aacgcacgcg
     1441 acgacctcaa tccgactgca tcccgcctgg cgtagagtgg ctcaagactc gagtcgttcg
     1501 cttcgagcct gcggagcacg cgctgcttac cgcggatggc cagtggcact cctacgagca
     1561 gctgatcgtg gcggtaggca ttcagctcga ttggcacaaa atcgagggtc tgccccccat
     1621 aagctccggc gtgatgggcg ccgacactgg tatttgcagc aactacagct acgagaccgt
     1681 ggatagcacc tggcaggcgc tgcaggcgtt ctcggagggg aacgcgctct tcacgctgcc
     1741 ggcgacaccg gtcaagtgcg ccggggcgcc gcagaagatc atgtacctga cggacgatta
     1801 cctgcgtctt cgcggcatgc gcgagcgcgc gcggatctgg tacgcggcca gcagcgcaag
     1861 catcttcggc gtggagaagt acgcccgcac acttcgtcag gtcgtcgagc gcaaacacat
     1921 ccaaaccctg tttcgccacc atctggtgcg ggtcgatgtg gcggagcgta aagccacctt
     1981 ccaacatctg gatagcgggg aacaacgaac cctcgactac aacctgctgc acgtcacacc
     2041 tccgcagagc gctcccgacg tcatcaaaca gagcccgctc gccaacccag acggttgggt
     2101 ggaagtcgac aaacacagcc tgcagcacaa acgtttcccg gacgtgtttg cgctcgggga
     2161 cgcgagcagc ctgcccactt cgcgcaccgg cgcggccgtg cgccgccagg ctccggtgtt
     2221 ggtcgagaac ctgctcgcgc atcgcgcccg gcaaagactc caggcaagct actcgggata
     2281 ctcgtcctgc ccgttgatta cgggctatgg gcgcctggtt ttggcggagt tcgattacga
     2341 cgggcagcct atggagagct tccccatcga tcaagcccgc gagcgctaca gcatgtactt
     2401 gttcaaggcc tatgccttgc cggcgctgta ctggcgcgcg atcttgcgcg ggcgcttgta
     2461 gcgcggcttg acgcccgagt gggggagctc caggtctccc gaggtgaaga gtccctcagt
     2521 tgcagtgctg ctcggctcgc tcctggtcac gtggcttccc ggctgcgatc gctcgaaacc
     2581 cgaggccagc gttgctccgg cgcctgccgc agcgccttcg gcggtgttat ccccagcggt
     2641 gtcctcctcg gccgcacaaa ccaacgtcag tccgtacacc ttgcaggtga agacgctcga
     2701 aggcgcagca ttgccgctgg agcaatttcg aggcagcgtg acgttgttcg tcaacgtggc
     2761 gagtgagtgc ggctacacgc cgcaatacgc ggggctgcag cgcatctacg agaagtatcg
     2821 cgcgcgtggc ttctccgtgc tgggctttcc gagcaatgac ttcggtcgac aagagcccgg
     2881 atcacccgcg gaaattcggg ctttctgctc cagcaaatac cgcgtgaact tcccgatgtt
     2941 cgcgaaggtc tcgacccgct ctggccccga tcaatctccc gtttacgcgt cattgagcgc
     3001 ggcaacgggt cagttgccct cttggaactt tggcaagtac ctggtgggtc gcgacggcaa
     3061 ggtcctccat ttctacgact ccaaggtgga gcccgagtca gccgagctca ccgccgccat
     3121 agaaggcgcg ctcgcggccg ctcgatagcg ttcagagtgg cggcaatgcg tgctcacgca
     3181 acaccgcgag ctcgtcatcg ctgatcccga tccccgccag gtaagcgccg attcccccgc
     3241 gcgcctcgag ctcatccaac atgcccgcca agctgtccgg gtaagctccc acgagtggca
     3301 gtgatagcga gtactcggcc atcacgtctt cgcgcgcgac cccgagggcg agcagcacca
     3361 gcgccgcgac gatgccggtg cgatcccgcc cccaagtgca atgcagatag accgggtaag
     3421 cgtcgggact cccgagcgta tggaacaccg ccgcgatcga ctcggtcgtg ttcagatccg
     3481 cgatgtagtc ttgcgtgctg acattatacg ggaccggcaa aggcgacagc tgaatactcg
     3541 cggtcgcgcc gacacattcc gagtctggtt tcgcgacccg ctccgactcg acccgcaaat
     3601 caatcacggt ccgaatcccg agctccgcga aagctccgca ggctgcactc gagagcacca
     3661 agggtggccc tcggaacacc gcgccacagg cgacgctctg gggctctgcc gctccttcgg
     3721 ccgcttcctc tgcaggtcgc gcgacaatcg gagtgcctcc taaatctcgc gcgttgacga
     3781 tttcgggcgt gaggatcggc cgtcccgctt tacacacgac atcgggcggc ccattcggcg
     3841 tctcgcccgg cttcacgcac tccgggtgcg ggttggtcgc gcactcgccc ggagccgtcg
     3901 aaccgttccc gccttccgtc cacgtcacga gcggtgggct cccgcagctg gtgagtccgc
     3961 acgcggccgc tacgagagct gcagagaaca gtaacgagtt gctcattcgc tggtttttga
     4021 ggggcagttc gagggccaag cgcggctgag tctactctcc cctgcgggca agagccacga
     4081 acaacgcagg acgtgattgc tgctcccctg tcaaattgca tggttcgagg acggccgtgc
     4141 tcgctaccgc tgcgcgcggc cgaggccctc aacgccatgc tcgcgcttcg cgctgcgctg
     4201 gcggagggtc ctccctctta tcttcacgcg gtggcgcgcg gcctcgtcac gctcgctcat
     4261 tgggggtggc cgcttaccaa cctcggggcg cggcacacga gtcgtgatca cccgcgctgt
     4321 gtggatatca gatcgagggg tgatgagcct cgggcccttc ttgcccgttt cgcacgcggc
     4381 gtgtcgagtc gatacgagtc gagcagatct gggtcatctt tcgagccgcc attgagctgc
     4441 tttgcctagc tctctcgcca agatgagata gaccagcgca cccaccgcac gcgcttcgag
     4501 gcacgtgaat cgtgagccat gacgagtctc gagttcgtga cgagtcttgc tgatcgaact
     4561 cacgccgagc cacgccccgt ggcggtggtg catgcaggag acccatgaga atcgcttcta
     4621 gtagacgagt ctggatttcc acgttggcgc tgattttcgt tgccgcttgc agcagcaacc
     4681 aggacgcgaa ctcggaccaa gaagtcctcg gcgtcgtgtc ggccgaagtg caatctgcgg
     4741 acaacctgat cacggccacc ctcaagctcg agacttgggc gggtggctac aacggcaccg
     4801 tcacgatcac caacaacggc agcacgccga cgtcttcgtg gttggtccgc atcgatccgg
     4861 gtggcagcag cgtgaccaac ctgtggagcg cggtggcgcc ggcgcgtccg atcaccgcga
     4921 cgttcgacgt caagaacgag agttacaacg ggtccattcc cgccaagggc tcggtgcagt
     4981 tcggcttctc cggcagcggc actggcaccc cgcaaatcct ctcggtctcg caggtgggtg
     5041 cgagcggggg ctcgggctcg ggaggtggcg gcagtggcgg tagcagcagc ggcggtagta
     5101 gcagtggcgg tgctaccgcg cgcggtggca gtagcagcgg cggaaccgtc ggcgctggcg
     5161 ggagtacgag tcgaggcggc agcgcgagtg gtggcagcgc gagcggtggc agcggcgccg
     5221 tatccaacac cggtaacccg tttgcaggcg cctcgctcta cgtcgaaacc aactcgctct
     5281 gcgcgcagaa cgccaagagc gatagccgcc tgaacacgat ttgccaaaac ccgcagggtg
     5341 attgggtcgg caattggagc ggcgatccgg cgagcgcggt ccgaacgctg ctcagcgccg
     5401 catccggcaa gctgcgcaca ctggtggcct ataacatcta tcggcgtgac tgcggcggcg
     5461 cttcgagcgg cggcgcggcc agcccggccg cttaccgcac ctggatcgac ggcttcgcca
     5521 gcggcatcgg caactcgccg gtcgccgtaa tcttggagcc cgatgcgctc gccttcaact
     5581 gcaacaacac tggtaacaac gactcgaccc tcgccatgct caagtacgcg gtcgagaaga
     5641 tcgaggcgca gcccaacgcc gcggtgtaca tcgacgcggg ccaccccaac tggatcagcg
     5701 tgagcgagac ggtcaatcgc ctgaagcaag ccggcatcgc gactgctacc ggcttctcgc
     5761 tgaatgtctc gaacttctac tccaccgata gcaacatcag ccatggtagg agtgtggcgg
     5821 cgggcgtcgg caacaagcct ttcgtgatcg acacgagccg caacgggctc acgtcctccg
     5881 ggtcggagtg gtgcaacccg cgtccgcgcg ggctcggcaa gcgccccacc ggcaacaccg
     5941 gtcaaagtgg cgtgcacgca tttctatggg tgaagcgccc cggcgagtcc gatggggcgt
     6001 gcaacggcgg gcccgcggcg ggccagtggt acaacgatta cgcattgcac ttgattcaga
     6061 acgcgcagct ttagctcgtg aatgaacgga gaaggcgagt tccgggtcag ccccggaact
     6121 tgccactctg cgctgccgcg cttcagaacg tctcgccgtc gaagccgaag aggtgagggg
     6181 tgttctcggt aaactcgaga tagatgcggt tcgagggcac cttcaagccg tgggtgagct
     6241 tttcgcacaa cactcggctc agctccgccg tgagcttggc gggcaacgcc ccaatgttct
     6301 tgatttccgc gtaacacgcg ggctcttcgc tgccggcgaa ggtcatgtgc gctgcggctc
     6361 gaggcaagtc atgacgtacg cttccggctt gttcaacagg cgggctaggg tcgtcgagag
     6421 ttcccggagg aactcgctgc gggcactcgg ggccgggtgc ggggcggagc tgacgatgcg
     6481 gatgacaggc atgtgcccga ggctacgagt cgccgcgcag cgtggaaagc acaaagcgcg
     6541 gctcggacaa gccattggtg ctggtcacga tcgtgggtcg gcgtgtcagc aaggccaatc
     6601 ccacgcccac ccagagcagc tctttggccc gcttgagcaa ggcgaacgcg gccacggtgt
     6661 ccacgctgtc gtttaccccg agcgcacgca gcagcagcgc gtaaccggcg tcttgcacgc
     6721 cgatcccggc gggcaacacc accaccaagc tgcgcagcag tgaaagcatg acctccatcg
     6781 cggcgatcgc gccaaagtcc agattcaccc ccagcaacct caagccgacg tacgtctcga
     6841 ccgactcccc caaccaccca aagacgaagc ccatggtgag ccaagcttca cggcccgcgc
     6901 tgaacgtgaa cagacgcgcc agctccgcgt ccgtgttctc gaagcgtgct cgccggctgc
     6961 acgccgagac tcgaaccgcc gtgccatgcg cgggagcccg ccgatgaagc acgctgggca
     7021 gctggcgtac acgatcccag gccgcgccca gccgcaccgt cacgttggca tgcacgagga
     7081 tgctgcgcgt tccgaaggcc aacaggagga gcgcgcaggc cgccgcaaag actccgagcg
     7141 cccacgagct cgggcccggg aagctcgccc atcggaagcc caacgcatgc gcggcgagcg
     7201 cactcaccac gatcgagtag acaccttgcg cggtgagcgt caggtacttc cgcgcgctca
     7261 cgagcgctac ggcctgggct ccctgtacgc cggagtgccg cattagcatc gggatcttgg
     7321 cggactcggc gaacaccacg cccgccggta agcagagcgc caaagattcc gtcgccaccc
     7381 gcacgaacaa caacgatgaa tagcgaactc ggtagcgtaa ccagcgggcg acgcgcatcc
     7441 agccccaagt atcgaccgca aaactcagcg cttgcgggag gagcaccagc ccgagtccgc
     7501 ccgctccgac ccgggcgcag aggtcgagaa catggctcac gctcacgtca cgaaaggtcc
     7561 accccaaagc tgcggccgcg ccggcaaggg ccagcacacg tgccacacgg agcatgggtt
     7621 tctgcggagg taagttcacg ccgcctacga ataggcgcgg cactttaccg tctggtggcg
     7681 ttctctcgaa ttaatggcgg ccgttcgcgc acgatggagt cgtggcgggc gggtgcgtca
     7741 aaaccaatgc agcgccacgt gagcaatcca catgaacgcg atgcagccgc ccaagatcag
     7801 aaatagcgcg taacgcccga tgaacgacgg cgggcgagac ggccgcccgg cagcgcggac
     7861 acgtggcgcc atttgattgc gggcccaatc acaggttctt ctgaatccca ccaaacacgt
     7921 attcacctcg atcggcgaat cgtcggtgag catgcggcgc aggtgaggtg tagcccgctc
     7981 gggcgccaag tcccggagca acatgactgc ccagacccgc ccggtggggg tcccttcggt
     8041 ggcgatccgc tccagctcat cctccacccc tggggcctca cggatggccc tcaagctgtc
     8101 tccataagga tcgattcccg gcatacccgg gttcaggttc aactggtccg cgcgcgcgat
     8161 cgcttccagc atcgcgccgg gggaggtgct cgaaggggtg gtcgtcaccg gtcgagttta
     8221 ccacccgccg tgcccggccc tagccccacc ccgtttggag cctccgtcgc accgccggct
     8281 cgggccagag cgtcgagcag cgagcgcgct ctctcctgat ccctacgtgc ccgctctggc
     8341 tgcccgcgtg cttccgcgag ggctccgcgc tcgatgagca atcgtgcgta gagctcgagc
     8401 cgttcgcgcg acaacatctt catcaagctt tcgaccgcca agtactcggc gtcccggctc
     8461 caagggccaa gtgcttgacg catcatttcg gagagctctc gctccgcctg atcgagctcg
     8521 ccggcagctc gtagccccgc caggcgagca aagaacttgg cgagatcctg gatcagccgc
     8581 cgaatcagat ccttttcgac cagagacatg cgcgaataat accagcatta tgccggccgc
     8641 ccgcttcctt cgataagttc gagtcgaaca ctgactcgag aggcctcgaa ccggagcccg
     8701 gcgtgaactc catatcgacg ctggaaaacc tctaccggca gacgctcacc tcgagttacc
     8761 ctcgagggat tctttggtgg agagagtgcg gacggacggc gaactgtgca tcaacggaaa
     8821 tagtgcaccg tcagtgaacc gcgaatcacg gcgcgcgaat gcagttagcg cagggaaaac
     8881 gagcgcgcgg ataaaccagc gcagaacaat agacttgaac ggcgaacaca cgatgagagc
     8941 gaacagcgtt gttggttggg tgaggggtga tatccacggg caaacaccgg gacggggcac
     9001 gcggctcgcg cgcggcgcga cgcggggaat tcgctgggcg ttagccgcgg cggcggcgtt
     9061 ggcgggcccg ctctgcgctt caggcgcgca agcggaagac ctgtcctatt caggcgtaga
     9121 tcgcgccacc gtgcgagtgc tggcgctcgg ccccgcgaag gtcgtgcccg tagagcatcg
     9181 cggcgtcgtt taccaaattg gcgtaccgct cggcgggcac ggcagtggcg tgttggtcaa
     9241 agagaatggg ctcgtgctga cggccgccca cgtcgtcgag aacgcgcgcc tgctggcggt
     9301 gcatttgccg ggcaagcgcg agcccgtgcc cgccaaagtg ctctacgtca acgacgccga
     9361 agactacgcc ttcattcgca ttgccggtac ctattcggaa attgcgcact tcgcggcgcc
     9421 ggacgaacag ctcaaagtgc gctccacgat ctttgccatc ggttatcctc tcgacgccac
     9481 gcgcttcgac cctcaatcga gcaaagggtc tatttcgggc aagcgccccg acgggcggct
     9541 ccagctcgat atccaggtca accccggcaa ctccggcggc cccgtaattg atgaaaagga
     9601 gcaggtgcaa gccatcgtcg tcgcgcgttc ggacgtggag aagggccaag ttggcttggc
     9661 cgcggccgtg ccggtctctt cgttccgcag caagtacgag gagctcgcgc aggccactcc
     9721 ggacacctcc gacatcgaca ccgaggccgc gcagcgcgtg gccaacatgg tctccacgtt
     9781 ggcgcaggac gggctcgagt ggttcaaggg ctctctcgag agcagcgaat ctcagaacga
     9841 catcaactcc cgagtccgaa agcaagccga ggacatgccg gcttcgtccg acgcccaaat
     9901 catggccgcg gtttatttct ggaatcgcta catggttcgc cttgcgcgag gccaagaggg
     9961 gcaagagcta catagccgcg cgacccagta cgtggagcgc gcggtcaagc tggagcctgg
    10021 cctgaaagag tcctcggact tcgtcgagtt cgtgctcggc gaggggcgag aagagcgcgc
    10081 cccgcagcac atcgggccgc gcccccgaca ccacgaaggc tttttcctgc gggcaggtta
    10141 cgggctcggc tatttgagct cgagcttgga gcagaacggt ggcgagagct tgtccggcct
    10201 gctcaagcaa tgggaggtgc tgattggcgg cggtggcagg gggatgtttt ggggtgccgc
    10261 cttctccaac gcctccggcg acgtggacta cgaacacaaa gacggaaatg ggaatacgct
    10321 caagtccacc acgcacgcga gcgtctttcg tttcggtccg ttttggcaat acttcccgtc
    10381 cgtcacgggt gggctcaatt tccggctcca agcctcgatc atgggagctg atgcatccgg
    10441 ggccagtctc aacggcggct tcgcggccag cgccggcgca ggttacgatt tgtggttctc
    10501 cagtgcattc aatgcgggct tgaacgtggg catcgcctac ttgtcaggct cacagaacag
    10561 cggctggctg cccacggcga cgttatccct cggcttgcgc taaacgccca tgctctgagc
    10621 tcgctccaag gagtaagcgc gccggtgagt ttgcgccggc gccctcaggc tagtgcgagc
    10681 gtccagcacg cgctcgcgca tcttaccgcc ccgcgtggat gcgctatcgt gcacggatgc
    10741 agttcctgct cctcgtggca ttctgcgcaa tctccgcgct cacgctcggg ctcgcgatcg
    10801 tgaaccgccg ctaccaggag cgacatgcgc acgtcgttcc tccggagctc caggccctga
    10861 gcgtggccca gggtcaggcg ttgttcgacg cggacgcgct ccgcaaggca gcgagctacg
    10921 cgcgcgatcg cagtcgcttc ggggtgcttc ggtcgattgc ctcgcggtgc atgctgctgg
    10981 ctttcttttt cgggggtggg ctcggcgtct acgaccgctg ggtttcaggg ctggggctct
    11041 ctttcatcgc ccagggggtc gtgttcgccc tcggaatcat ggcgttgtcc gcggtctggg
    11101 agctgccgtt ctctgtttat cggaccttcg cgttggagca aaagcacggc ttcaatcgca
    11161 tgacccccgg gctattcatc agcgatgcgc tgaaggggct cgtgctcggc ggcctcatga
    11221 tcgcgggctt gagcgcggcc gcgctgtggc tcgttcggtt gctgccgaat gggtactggc
    11281 tcgccatctg gggcttgctg ctggcgttca acgtgttcgt aacgttgctc gcgccttacg
    11341 tgatcgagcc gttgttcttc aagatgaccg agctagccat ccccgagctg ggtgagcgca
    11401 tccagcgctt ggccgaccgc gccggggtgc gggtctcgcg cgtgttgaag atggacgcct
    11461 cacgacgcag cagccactcg aacgcctact tcacgggctt cgggcccgtg aagcgggtcg
    11521 tgctgttcga tacgttgatc gaacgaatga tgccggacga aatcctggcc gtgctcgcgc
    11581 acgaactcgg ccactggaag aagcgacaca ttacccagcg cttcttactc ggcgaactgg
    11641 cgagcttcgt cggactgtgg atcgtcagcc tgctggtggc ctggcccgcg ttgccggagt
    11701 gggtgttctt gaatgaagct tcgctaccgg cgcgagccgt aatcgtggcg ctggcctggt
    11761 cggtgttctc gttcccactc acgccgctcg ccagcgcctg gtcgcggcgt cacgagcgcg
    11821 aagccgatcg ctttgcgcgg gacctcacgc agacgccgcg cgcgctggcc accgcgctcg
    11881 ccaagctcgc gcgcgataac ctggcgagct tgacatatca tccttggttc gaggccgtgt
    11941 acgcctcgca tcccagcgtg gtggagcgcg tacggacgct cacctccgcc tcgcttcacg
    12001 cggcaggcga aacctctgct gcgtaggtgg cgggcgcggg gctcgcgcga ttcacggctt
    12061 ctttcagctt ggcggccagc acgcgcacgt tgggctcttc gcacatggtg ctgtgattgc
    12121 ccgggcagag ctctacgtga accccgccga gtgcgtagcg agaccagccg tagcgttcgt
    12181 cgacctcgag cgccgtccat agcgtcagcg cactctcttc ccgcgctcgg aacaaatgca
    12241 cctggccatt ccagggcgtc ggtcggtaac gttgttcggc cagcacccag gagtccgcca
    12301 actgttcgta gcggtagcgc tcggggaaga ccttgcccaa attccgcgcc atcacgcggc
    12361 cgagattcca actctcgtag gtgagtcgcc gcccgagcgt ctcgaccaag taactcggcc
    12421 gatcgatgac tcgcttggcg tgcatgcggg cccgttgcac gaatgggcgt gtcggtaggt
    12481 gcgggctgaa ggaatcgatc aggcccacga acgcgacgcg cttgccgcgc gcctccagct
    12541 gcttggcaat ctcgaacgcg gctacgccgc ctcccgagta gccaccgagc aaatacggcc
    12601 catcggggca cacgctcgag atctccgaga tatagtgctc ggcgagcgcc tcgacggtgt
    12661 acaaaatctc ggtctgcccg tctgcaccgg gcggctgcaa gccgtagaac gggcgctgat
    12721 ccccagccaa taacgccagc ttacgtaggt tgttcagggt gccgcccatg cccgctacgc
    12781 agaagatggg aggcaactcg ccatgcggtt gcatcggcac gagcgagcgc cagcgcgttc
    12841 ggcgtacgtg cgaggcctga ccagagctgt gcgctgcgtc gccggaggcg gcgtcgccgc
    12901 cgtcgccgga gccgacgccg ttggggcccg cgggcggagg aggggcggag tcactcgcgc
    12961 gaaagcgcag ggaggggggc ggcgtttcgt cctccgcgcc cggagccggg ggtggagatg
    13021 aaggcatcgt cgcgggcccc gaagccgaag tgctctcggg ccccgcgttg gcgttggccg
    13081 cgatcgcgag cggggccggc gtgatgccca gttcggaagc gatcacctcc gcgcattgag
    13141 ccaccgtcgg cgcctcgaac agcgtggaga gcggcaggtc caccgcgtgt ttgcggcgga
    13201 tcttgttgaa caagcgcact gcgaccagtg actgcccacc gagctcgaag aagtcgtcgt
    13261 gaatgcccac gtgctgcacc cccagcagtt cactgaacaa ctcactcagc tcgagctcca
    13321 gctcgttgcg cggggccacg tagttggcgt gcactgcgcg tgacgtctcc ccgtcttcgt
    13381 tcaaggcggc gctcgggtcc gcggggcggc tgctgtgctg gcttgccgcg cgagcctcca
    13441 gcgtcttggc gtcgaccgag cgcagccact ccgcaatcgg aactgaactg gcgatccagt
    13501 ggcccgagcg tggttgcttc agcagacgct cgaagacctg cactccctcg ctcggcaaga
    13561 tgccgtgcgc caccacctgc gagagctggc tgcgcggcga ttcgtgcgca cgtggcgccc
    13621 gcgtcaaccc cgcagagcgc tcggtaggca gcgtggccga cgtgactcct gtgcctgacg
    13681 cgggcaacgc cgcgaatcgg ccctgatcga cgcgcgtcat gcagaagttc tcgatctcca
    13741 cgagcacttc cccctgcgct ccatgaatcg tcacatcgaa gctcaccagg gccggagtgt
    13801 tccccgtgcg caagcgcacg tgactgaaga cgtggctcgt gagcggcgcg accagtcgga
    13861 ccttgccgta agacagcggt accaggaagt ctcgcccgcc gtcgaagccg ggaaccagcg
    13921 cctgcgcccc gccggtcgcc atatcgagca acgccggatg tagctcgtag ctcgccagat
    13981 ccgcactgaa cagcgcggcc aatttgagct cgatcaaggc ttcacgggcc ccgtccgagc
    14041 tgctcagccg cacgctctcc acgtttgccc aacgtgggcc gaagtccatg aacggctgcg
    14101 gcaggaaacc tccgatctgc tgatcgggcc cccgcaaccg ttcgcgcaga gccgccagat
    14161 ccacggcggg ccgctcctgc ttgctcagcg ggcgcgcgct gcccgtggcg tgcggctctc
    14221 gctcagactc cgagaagaac gtgaagcgca cctgatcccc agcgcccagg gtcagcctca
    14281 agcgcaaatc acggctctcg ccttcgccca ccgtgaaggg cgagaggaag gccacgttct
    14341 ccagctcgag cgcgcgcgtg cggagtgcgt gtgagaggcc cgcgcgtgag agctctagat
    14401 agccagtgcc gggcagcagc gctgcccctc cgcgcaccac gtgttccccg agtggccaat
    14461 gccgaactcg ggtgaagcgg gtgacgaaca gcatctcttc gggggtgtcg accgcgatgc
    14521 gctccaagca tgggtgggga ccgatccggc cgtgctcggt cactccgccg gggcgcgccg
    14581 cctccgtcac ctcgacggcc atgccgactt cttgccaggc gttccaacca atggaaatcg
    14641 tcagcgggcg gccgggcccg agctgggcat tcgcgtaagc gtccaagaac gcgttggccg
    14701 ctgtatagtc gatttgccct tccagcccga gcacgctgct gacggacgag aagagcacca
    14761 cgaaatccaa gcggtcgcgt ccgaacaccc gatccaggac caaggtgctc ttcaccttgc
    14821 tcgcgagcac tcggcgcgcg ctgtcctcgg tcttcagaga aatcagcgtg tcgtcgagcg
    14881 ttcccgccgc gtgcagaatg ccgtccagtt tgccgaagcg ctctcgaatg cgttcgcgca
    14941 gcccgctcat ctgctcgaaa tcggcggcgt ccacgtgcgc caacatgacg tccgcgccgg
    15001 ccgcctcgag ctcctgaatc gcgcgcagct tccgcgcgat cggatcgtcg acggtgtgct
    15061 cggccaagta gcgagctcgc tccgcggggc tcggtagcac ggtgcgtccc accagcacca
    15121 agcgcgcgtg cgcttgcaaa gccaggtgtt tggcgaaggt caagccgagc ccaccgaagc
    15181 cgcccgtgat cagatataca ccgcgcggcc gtactcgaac gggggcgccc gagatgctcg
    15241 cctccgacga tgactcgacg ggcttcgccg ggatcacttc ctgcacgaag cgcatcccgc
    15301 cacgcagcgc tacgatcggg tctgccgcgt gcgtcggggc cggagctcgc gacagctcgt
    15361 ccgcgagctc ttgggccaag cgatccagct cggcgtcact gccccggtcg caactcacat
    15421 cgatgcagcg cgtgcgcacg ctcggcaatt ctcgcggcaa cacccgcacc gcaccgagca
    15481 cgagtgcttt ctcgggatgg ggcgccggat cgttgcccac cttgtgcagc tgactcgtga
    15541 ccacgtcgag ccgcagctcg agctcacgct ttgcgacttc ctgactcaaa tacagcagac
    15601 tcttgaaata gtgcgcttcg tgtccctcgt acgcttccgg cgctagcgcc gcgctccgcg
    15661 tggagagccc caatcgattg tgattgggcg tgactccgaa caaatgcacg atctgatcgg
    15721 gcagccgctg cgtggcttcg agatcctcga acagcgccgc gtagtcttcg cgcgcgtcca
    15781 ctcggagcat gtagcggtcg gccgagagct tcagaaagcg ttccccgggg gaaacgtgga
    15841 tgttctcggg cgaacccgcc tcgagccgcc ggtgaagtcc cagcttgtcg gtgaagaaca
    15901 atcgccgtcg cgccggcacc gtgggccccg cctcggccag ctcccggaat acacacttgc
    15961 cgaaccagct ctcgagctgc gcttgtttgt gcgggcgaaa caccttggcc gcttgcgcgc
    16021 tcgcgttggc gttcgccgga atgaaatagc gcttgagttc gaacggatag gtcggcagcg
    16081 gcacccgtcg tcgcgcgccg ctcatcaggg cgctgaaatc cagctcacca ccggccaccc
    16141 agagccgccc caagctctcg agcaggaatt cgcgatcttc cgcgctctcg cgcgggtgtc
    16201 gcatcgtcgg ggtcgcgaac ggcacgttgg gttgctgccg tgccaagctg gccagcgtgc
    16261 gcccgggccc tacctcgagc aatagcggat tgccctcggc gagcaacgtc gagatccctt
    16321 gattgaaccg caccgtgctc cgcaagtgtg ccacccaata cccgggcgtc ttgacctgct
    16381 ctgggtccac ccaactgccg gtcagattcg atatgaacgg tcgcttcggc gtgtggaagc
    16441 ggatactccg gcagtagctc tcgaactcac gcagaattgg ctccagcatg ctggagtgtg
    16501 cggccaccga gatgtggatt cgctgcgtct ccaccccgcg ctcattcaga atttgctcga
    16561 gcctctggat tgcggcgacg gggcccgacg ccaccgagag ttctggagca ttctccgctg
    16621 caatcgaaag ctccgggctc aaatgccgac gcagctcctg ctccggcagc tggacgctga
    16681 gcatagtccc ttcgggcagc gtctcgaaca gccgccctcg tcgcgtgacg agccccatcc
    16741 catcggccgc gctgaacacc tcggccaagc tcgcggccac gtattcaccc atgctgtgcc
    16801 caatcaagct caccggctcg agcccgagcg acatcaacag cctggccatc gcgtattcgg
    16861 tcgcaaatag ggcgggcaac gccaggctcg gccgctccag ctcctggctg gcttgctggg
    16921 gattcacgcg ctcgagcaac aatgcgctca ctttcttgcg atgctccgag tccagcgcgg
    16981 acaggcactc gtccaccgcc ttccgataga cgggctcgtg cgagtacaac gccgctccca
    17041 taccggcata ttgagcgccg ccgccgggga acatgaaggc gacctcgttc ttaccgctac
    17101 gcgcggtgcc gctctgaatc cgcttgggat cggggctgag cagcgcggcc accgcgtccg
    17161 cccggtcacg agcgacgaag gcgagccgct tattgaacgc cttgcgacct tgggccagcg
    17221 tgtacgccac gtccgcgagc ggcgcggcct cgtgcccatt caagtggtcg gcgagcgcgg
    17281 acttggcctg caacaaggcc gcgtcactgc gcgcactcag caccacgagc tcgggccccg
    17341 cttccccggc cttggcttgg ctgacgggcg ccgcctcgag aatcaaatgt acattcgttc
    17401 cgcccgcgcc cagcgccgta atccccgcac gcgcgggaaa acctggcgtt ggaatgggga
    17461 agtccgcgag cgcggtattg acgcgaaagg ggctcctcgc aaaatcgatc tcggggttcg
    17521 gttgcgagta attgatgctt ggcaccactt gcccgtgcct caaggagagt accgtcttga
    17581 tcagccccgc aatgcccgcc gcttctccca ggtggccgat gttggccttc aacgagccaa
    17641 tcgcgcagaa gccgcgccgc gatgtgtgcg gcgcgaaagc ttcgttcaac gcacgcacct
    17701 cgattggatc gcccacggcc gtgccggttc cgtgtgtctc gacatatcca atggtctccg
    17761 cgtgcacacc cgaaatcgcc agcgcctccg agatcgcgcg cgcttgcccc tccacactcg
    17821 gcgccagata acccaccttt tgagccccgt cgttgttgat cgcggagccg cgaatcaccg
    17881 ccaaaatcgt gtccccgtcg cgctccgcgt cggccaaacg gcgcaacacc acacaacccg
    17941 tcccgctgcc gaacagcgtc ccgtgtgacc cggcatcgaa tgggcggcag tgcccatccg
    18001 gtgagagaat ctcaccgggc ttgtacacat agccgcggtc ttgcggcaaa gacagcgtgg
    18061 acgcccccgc cagagcgtag tcgcattccc ctgcgagaag actctggcaa gccaagtgca
    18121 cggccgtgag cgccgaggaa cacgccgtct gcacgttcag actcggccct ttcagattca
    18181 tctgataaga cgtgcgcgtg gccaggaaat tcatgtcatt cccgttgtgg cgcaccagcc
    18241 actcacccac tgtttgcatc acctcctgat tcgtcaccag gtgatacatc atgtagtgat
    18301 tcatgccgca ggccgcgaac accccgaccg ggcccagcgt gcgctctggc tcgtatccgg
    18361 cgcgctccaa cgcctcccag gccaccatca agaacagccg atgctgcggg tccatcaccg
    18421 ccgcgtcccg cgggctcatc ccgaagaaac ctgcgtcgaa atggtccatg ttctcgagca
    18481 cgggccaagc gcgcacgtag ttcggatcag ccaaattctc gggcgactca ccgttcctca
    18541 gcagctcctc gtcactgaag aagtgcacgg actctagacc gcgcttcaga ttttcccaaa
    18601 gcgccgcggg gctcggtgca cccgggaagc gcccggacat accgacgatc gcgatctggt
    18661 agccttcgtt ccgatcttcg aattcctgac tcactgcgtt ctctcccatg gcgtgaggcc
    18721 cagcccctgt ctgaagaact cgaccaaatc cgcggcgtag ctgcggctgc gactggcgta
    18781 accaccgtgt ccgttgcccg gcaccacgag tagcgccttg ggctggcgtg cggccgcgaa
    18841 catggtctcc gccattgcgc ggggcacgag ctcatcttgt tccgaagtca cgattaatag
    18901 ggcgcgcgga gcaatctcgg gcacggcagc atgcgccgaa tctcgctctg gatcatgccc
    18961 agactgcaga aaagccaact tcgcgcaggc cgctccgaat gtgccccatc gacggtgagt
    19021 gtggcgaatt cgatccccga ggtgagcagg agtgccctcc aacgccaccg cccgaatccg
    19081 aacatcgctc cgagcgacgc gcgccaacat ataaccgccg agtgagaaac ccagcgcacc
    19141 gagcctctcc gcatcgactc cgggctgccg gagtaagaat tggactgcgt ctcggatcgc
    19201 agtttcctcg ggccggcccc aacgtacgtc gccctcgctg cggccgtgtc cggggagctc
    19261 caagagcagc accccaaagc cgccctcgtg cagagcgcgc gcttccgcgg ccagcgacgc
    19321 gcgattgccg ccggccccat gaaataatgc aatcgctcca ccattccgcg caggtaagta
    19381 ccagccgtac agccgctcac cgttgctctc gaacgagacc tcgcgtgcgt cggcgagccc
    19441 cacatcggct ggcgacccgg cgagtgtggg cttcggaaaa cacatctcac gctccacccg
    19501 ataacttcgc cacgccacga acagcgccgg ggcaccgagc agcgcgagca gcgcggccat
    19561 cgcgaatcgc cggtagggac gcgcccggcg cgcattttta ccgcgtctcc gtcccgactc
    19621 gccccctgga tcgccgggat ctccggaggt ttcagagcgc tcactcacgc cgcgcgccgt
    19681 ggactcgaga ggttcggaca cagcctttca agatgcctga gcatgacccc aattgtcacc
    19741 tctgcaatac gagattgccc catcgaatcg actactaaga ccctattcca accggagcta
    19801 gacgctggtg gccaatggct cacgggtttc gcgggcggtg ctgtcgccgg cgttcagggc
    19861 ttcgccgttc ggccttcacg agcagtgacc cgcgctccgc gcgacggaat cggaggcact
    19921 atctttccac gcaagggggg aaagccaccc gacgcagttc cacgagcggg ggcactgagc
    19981 cgccgtgggt cacgacggct cagtctaggt tatccgagcg agccatgtta tctgcggcgc
    20041 acatgtcaat gacatctgct ttgcactgct cagtatcatt gcccggcgca gctctgcgta
    20101 gcttgagcct cgtactgggc ctggctgccg taacggcttg ctcctctgat tcgaactccg
    20161 ggacgaacac gcccgcgtca tcctgcatct tctgcgatag cttcgaatcc tatgcggccg
    20221 gtcccgtcgc cgagacgccc gccttcacgc ccaccaccag cggcggcacc ctcacgtatg
    20281 acgccgtgca cgcgcgggac aagcaagctt tgcacgtcca caccgacggc aacggtcgcg
    20341 cgtacatcgc gctcaagaac ttcaccccgc ccaacaacag cttttggggc cgtatgtacc
    20401 tgtgggtcga cgccttcccc accgcgccca actacgccca ctacacgctc gtcgaagcgg
    20461 ccggcacccc cgccggcttc gttcgcccca tcggtggtca atacatcatg gaggagggca
    20521 aaaacatgtg gggccccggc agcgacggcg gcaaaaccgg cgactggacc aactggcaaa
    20581 cctccgcccc cgccgaatcg ggcaagtggt tgtgcatgga gtggcagatg gccgccgccg
    20641 acaactcgat caagatctgg atcgacaacg tcgaaaagac cgatctcgcc gtctccaccc
    20701 aaaaccacgg cggcacccag tttgacttcg aattcccagt gttcgacaag ctgtggctcg
    20761 gctggtggct ctatcaagcc ggtcccacgc cggaccactt cgacatttgg atggacgacg
    20821 tggcgattgg caccgagcgg cagagctgca agtaacgacc ccttgcgccc aagttctgcg
    20881 gtgtaaatga gggggcgcca catccgaacg ggcgcgaagc ccgtcgtgtc gcgccccagc
    20941 gcagcctgct gcgcgcagag ccttcgattc accgcgccag cgcccgcgtc ttaccccgac
    21001 gtcaagtcac tccactcccg ctgccactca ccaacaccgg cgcgcgatac cccggcggtt
    21061 tatcgcccac ggtcgctccg ccgtacatca tgccctgcat ggcattcgtg aagaagtagt
    21121 acgggaacgg cgttggcggg cggctcttct cctcgagccg agcgctcagt tccgcgggga
    21181 tctcgaactc gagtgctcgc aagttactct ccaattgctc cggcttactc gcgcccaaaa
    21241 tgacgctcgc cacgcccggg cgccgcgtaa tccaattcag ggcaacttga ttcatcgggc
    21301 gtccgagctc actcgccact cgatcgagct ccgccacgat ctcgaaattc cgatccgtca
    21361 gatgctgaaa ggcggggttt ccactgccct gcatttgggc gagtcgcccc tcggttttcg
    21421 cctgcttcgc gtcgctcgcc cggtacttgc cagaaagcaa gccgctcgcg agcgggctcc
    21481 acgcgacgat acccatgccg agctcggtcg ccaagcgcgt gtactcgtgc tccaagttgc
    21541 gttccacgag cgaatactgc agctgcaggg cactcacggg ctcgaagccg cgccactccg
    21601 cgagcgtctg cgcgcgcgcc gcgtaccagg ccggcacgtc cgaaagcccg atatgccgaa
    21661 ccttgccgct gcgcaccaaa tcatccagcg tgcgcatcac ctcttcgctc ggtgttactt
    21721 ggtcccaagc gtgcaggtaa taaagatcga gatagtcggt gttcaatcga cgcagcgaac
    21781 cttcgatcgc gcgcaggatg ttcttccgcc cattgccgcc gatgttcgcg tttcgctcgc
    21841 ccaagttgaa cgtgaactta ctggcgatca ccagctggtc gcgcaagctg cgtgcgcgca
    21901 cgaactcacc gagccacagc tcactcgtac cattcgtata cgcatcggcg gtgtcgatga
    21961 aattgccgcc cgcctccacg taaacgtcga agacgcgctg cgcttcgggt ttggccgaac
    22021 cccaacccca ctccgttccg aaagtcatca cgcccagtga cagccgtgaa acgcgcagac
    22081 cactgcggcc caacgtgtag taatccgaaa tcgtcatcgt gctctccatc tttccctgcg
    22141 agcagagctt gtgggcaatt gccgccaacg tgaagtggcg agcctctttt cgaggtgtta
    22201 acgtccgctt atcaatgaag ctcgaccagc tggatggcgt gctcgccttc gtgacggtgg
    22261 cgcagaaacg cagcttcagc gcggccgccg cggaactcga ggtcactccg cccgccatct
    22321 cgcaagccat caagcagctg gaagaccggc taggcgtacg cctcttcaac cgaaccaccc
    22381 gcagtgtatc cctcactgaa gctggggctt gctttcacgc caaagtcgcg ccggctttgc
    22441 aacaaatcgg ggaggccgcc cagggtctcg acgacttccg ctcggccccg agcggcctat
    22501 tgagactcaa cagctcgcac gttgcgtggg aaatgctgtt gcgggaggct gttcgcagct
    22561 tcctcgagca acagcccgac attcgcatcg aagtccacct cgaggacgcc ttcgtcgaca
    22621 tcgtcgaagg cggctacgac gcgggcctgc gcctgggaga ctccgtgcaa cgcgacatgg
    22681 tcagcactct gctcacccgc cacgagcgct tcgcgttggt ggcctcacca gactacatat
    22741 ctcggcgggg tgctcctgcg cagctgaatg agctgcggga ccacgcctgc attcgattcc
    22801 gcttccgcgg ggcgggcggc atttaccgtt gggagctcat cgatcgcggg gaactcttgg
    22861 agatcgaagt tcagggcccg ctcacggtga gcgacagccg cagcctccgc gaagccgccc
    22921 ttgacggcgt gggcatcgcc cacaccttct tgcgcctcgt cgagcccgac ctcgcgcagg
    22981 gtcgcctagt cgccatcctc ccagaacact ggcccacctt cgcgggcttc tccctttact
    23041 acccgcatcg cgcgcaacaa cccgccaaac tccgcgcctt catcgaccac tgccgcaccc
    23101 taaccgcccc ccaccgctaa cccctccgcc ttcgcgggga gcgtgccctt gattgggcag
    23161 gtgtcgtggg ggcgttcagt ttgcgggggg ggcgcgacgc gggaggggcg tgtgagtaac
    23221 ggagcgcgcg ttacgtgttg gcgcgtgcgc ggtctcgcgg ggaagatgat gggggcatgg
    23281 gtctgcgcga gtgggcggag ttgacggggg tcgcgctcgt tgcagcgggc gcgcggggag
    23341 cgtgcccttg attgggcagg tgtcgtgggg gcgttcagtt tgcggggagg cgcgacgcgg
    23401 gaggtggcgt gagtgacgga gcaagcgtta cgtgttggcg catgcgcggt ctcgcggggg
    23461 agatgggggc a
//
LOCUS       DASTKU010001060         5385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1103311, whole genome shotgun sequence.
ACCESSION   DASTKU010001060 DASTKU010000000
VERSION     DASTKU010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103311"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFQ61_37720"
     CDS             complement(<1..573)
                     /locus_tag="VFQ61_37720"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET9960309.1"
                     /translation="MPLFKPGTLGFISLGLVCGAALASCGATDGEPAQQRVQRISGGE
                     QGDTWSAVFGTFMHEQDGVAGCSATLIAKNLLLTARHCVSFTSSRRVICGKSTLGEPV
                     SGDALIAANIPDLARSSRSDWFRGAEVYVPEEGDDSCGFDVALIILTENVPDSVARPA
                     IPRIDREVSAGERYVAVGYGLDELGGSGERR"
     gene            703..1494
                     /locus_tag="VFQ61_37725"
     CDS             703..1494
                     /locus_tag="VFQ61_37725"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET9960310.1"
                     /translation="MVASTKAIRIFQNIPVRPSRLFQALTDPKDLAAWHADVVRGKCE
                     VGGSLELSWPRLGVEIQAAVREVLPGKRVSLESSLGRIVMSIVPGGVELVHHGRFDAD
                     ERAGSESAWRVSLSLLATYLTRHVDRPRRVHWCLAPVRASAELCHAYFTERELVSRWL
                     GRMESSLGEAGSGVHCTLNSGRRLSGPVLSNTPGRDVALRWREADDSVLSLRTLRGSP
                     DEGQARYLLVGWSRWGDLPEAATLAREIDEASERLRRELDRLAFA"
     gene            complement(1521..2573)
                     /locus_tag="VFQ61_37730"
     CDS             complement(1521..2573)
                     /locus_tag="VFQ61_37730"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HET9960311.1"
                     /translation="MAARLIIVGAGSVGTSLGARLLKAGRDVAFVVRRPEQAEALKRH
                     GLSLEDPASGERFNVTCPVTLGFEALEAEHPSHVLLCVPATETAELAPALAQRFPHLP
                     IVSVQNDVDNESKIAKHHSRVIGAVWRQTCTRISDHEVRALGRARVILGAYPEGEGED
                     VQRLATEFRAAGLDVAISGRIMQDKWLKFCVNLMSAPNALVRPEQHVQSLFVLGKVGL
                     LAEAREVLSRAEIVTDSCDGKDRSIAEEIEFQKASLARGTSSRRVPVFNQVWRSLKER
                     SAASSRARGPSEAECYHERIITLGAQSGVPTPLNQRMKKCVAEAYEQKLGPECFDVTQ
                     VFDENEVEINTRTLGG"
     gene            complement(2734..3639)
                     /locus_tag="VFQ61_37735"
     CDS             complement(2734..3639)
                     /locus_tag="VFQ61_37735"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9960312.1"
                     /translation="MVAVRDFCIDRFEVRTVDARTHSALSPYYPPDAKLVASIFDGWQ
                     LEKLRVGTNSAREMPLPEVPWVQRSRSDYVPRAVSEAGVVPQGYLSYWLAKKACENAA
                     KRLCTENEWVTACKGQSGAKFPYGSSYDPNACNVYRAVHPATALHGAAFYGHRDPRLN
                     LVSEPSGAPLLRLTGSSPACRSRFGDDAVFDMVGNLDEWVEASPTSETSSGSSAKAVS
                     SRGRGHSSQRHAAARGGRLLQGAGPSTAGPSAAGSPAPNIGAFLGGFYARATREGCEA
                     RVGSHAPAYYDYSTGTRCCRAQGEQ"
     gene            3994..4689
                     /locus_tag="VFQ61_37740"
     CDS             3994..4689
                     /locus_tag="VFQ61_37740"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HET9960313.1"
                     /translation="MPWRKTTGGPAEASRANDASPASETRARHQLVQDIEGHAEIHDP
                     RILAAFERVPRHLFLPQTHRSYAYRDSALPIGFQQTISQPSMIAIMLEALELDPQHRV
                     LEVGAGSGYAAALLGELVREVHSVEIVPELAEQARARLQALEYRNIFVHHGNGRSGWW
                     DQAPYDRILVSAGAESVPPELIHQLVPGGILVMPIGDQSGQRLLIAHKDAAEGMHFSE
                     SIPCIFVPLVSTN"
     gene            complement(4699..5274)
                     /locus_tag="VFQ61_37745"
     CDS             complement(4699..5274)
                     /locus_tag="VFQ61_37745"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET9960314.1"
                     /translation="MSALGPSALAPGFLVAAPPLGDPNFDRAVVLLASHGADGAFGWI
                     INGRDVMTMSELLLRADISKDPPPRSGSVRLGGPVSKEQVWLIYREEDRFSEVDGQFE
                     VGAGVLSCASRRALELIALREPPASLFGVAGYAGWAPGQLENEIRSGAWLPVDLDRRL
                     VFEVPRDELWRRSYERAGTSPIAFSSKVGLA"
BASE COUNT         1000 a         1793 c         1647 g          945 t
ORIGIN      
        1 ccgccgctcg cccgaaccgc caagctcatc gagcccatat cctaccgcca cgtagcgctc
       61 ccctgccgaa acttcgcggt cgatccgagg gatggcgggc ctggctacgc tgtcggggac
      121 gttctcggta aggatgatca gcgccacatc gaagccacaa ctgtcgtccc cttcctccgg
      181 cacgtaaacc tctgcgcctc ggaaccagtc tgaccgcgag ctccgcgcca aatcggggat
      241 gttcgcagcg atgagcgcgt ccccgctcac cggttcgccc agggtggact tgccgcagat
      301 cacacgcctc gaactggtga acgaaacgca gtgacgcgcg gtgagtaaca agttcttcgc
      361 gatcagggtc gcagaacaac cggcgactcc gtcctgttcg tgcatgaacg tcccgaacac
      421 cgctgaccaa gtgtccccct gctcgccgcc cgagatccgt tgcacacgct gttgtgcggg
      481 ctcgccgtcc gttgccccac aactcgcgag agcggccccg caaaccaacc ccagcgaaat
      541 gaaaccaagc gtgcccggct tgaacaacgg catgtgcgct tgaatctatc acgcgaaacg
      601 cgatcggccg ccccggtgcg gggtatgacg cgggatcttg gtgtaagctc cgcccgcttt
      661 ccctggtctt atttcttccc ccgaaattcg aactcgtcac tcatggtcgc gagcaccaaa
      721 gccataagaa ttttccagaa catccccgtt cgtccgtcac gcttgtttca ggctctgacg
      781 gatccgaaag atctcgcggc ctggcacgcc gacgtagtgc gaggcaagtg tgaggtcggg
      841 ggctcgctcg agctttcgtg gccacggctc ggcgtagaaa tccaagccgc ggttcgagaa
      901 gtgttacctg gaaaacgggt cagcttggaa agcagcctgg gtcggatcgt gatgagcatc
      961 gtgccggggg gtgtggagct cgtgcatcat ggtcgattcg acgccgacga acgcgcgggg
     1021 agcgagtccg cgtggcgcgt ctcgctgagc ctactcgcca cgtacttgac cagacacgtc
     1081 gatcgcccgc gtcgggtgca ttggtgcttg gctccggttc gcgcgagcgc ggagctctgt
     1141 cacgcctact tcaccgaacg cgaactcgtt tcgcgctggc tgggtcgcat ggaatcgagc
     1201 ttgggcgaag cgggaagcgg agtgcattgt accctgaaca gtgggcggcg cttgagtggc
     1261 cccgtgctct cgaacacccc gggccgcgac gtggctttgc gctggcgcga ggccgatgac
     1321 tccgtgctca gcttacggac actgcgaggt tccccggacg agggccaagc ccggtacttg
     1381 ttggttggtt ggtcgcgttg gggagattta ccggaagccg ccacgctcgc acgcgagatc
     1441 gacgaagcga gtgaacggct gcgtcgcgag ctggaccggc tggcatttgc ttgagctcgc
     1501 gagggcgagc gcgccgtgag ctagccgccg agggtgcgtg tgttgatctc gacttcgttt
     1561 tcgtcgaaca cttgtgtgac gtcgaagcac tcgggcccca acttctgctc gtaagcctcg
     1621 gccacgcact tcttcatgcg ctggttgagc ggtgttggaa ccccggactg cgcgcctagc
     1681 gtgatgatcc gctcgtgata gcattcggcc tcactcgggc ctcgcgctcg actgctcgca
     1741 gcggatcgct ccttcagcga acgccacacc tgattgaaaa cgggtacacg ccggctgctg
     1801 gtgccgcgag ccagagatgc cttttgaaac tctatctcct cggcaatcga gcggtccttc
     1861 ccgtcacagc tatcggtgac gatttcagcc cgcgaaagca cctcgcgcgc ctccgccaag
     1921 agcccgacct taccgagcac gaatagggac tgcacgtgct gctcggggcg taccagcgcg
     1981 ttcggcgcac tcatcaggtt gacgcagaat ttcagccact tgtcctgcat gatgcgcccc
     2041 gagatcgcga catccaaacc ggccgcgcga aactcggtgg cgagcctctg tacgtcttct
     2101 ccctcacctt cggggtaggc gcccagaatc acgcgcgccc gtcccagcgc gcgcacctcg
     2161 tgatcgctga tgcgggtgca ggtctgtcgc cacaccgcgc cgatcacgcg tgagtgatgc
     2221 ttggcaatct tgctctcgtt gtcgacgtca ttttgcacgc tcacgatggg tagatgcggg
     2281 aaacgctgcg cgagcgcggg cgcaagctcg gccgtctccg tagccggcac acaaagcaga
     2341 acgtggctcg gatgttccgc ttccagagcc tcgaagccga gcgtgaccgg acaggtcaca
     2401 ttgaaacgct cgcccgaagc cggatcttcg agagacaggc catgcctttt cagcgcttcc
     2461 gcctgctcag gacgccggac tacgaaggcc acgtcgcgac ctgccttgag cagacgcgcg
     2521 cccaagctcg tgccgacgga tcctgcaccc acgataatca agcgagcggc catgctgctc
     2581 ttttagcaga cgcgacgtgc ttcgcgtccc ccgaggcaac acgagcatgc gtgagctatc
     2641 ggcggccggc gactagctcg tacttgggat agctcttatc gcggcgctgc ttcccaatcc
     2701 gtgattggcc tgagtgctgc cccgttctcg gctctattgc tcgccctgag cccggcagca
     2761 gcgagtgccc gtcgaatagt cgtaataggc tggagcatga gagccgacgc gagcttcgca
     2821 gccctcgcgc gtagccctcg cgtaaaaccc gcccaggaac gcgccgatat tcggagctgg
     2881 gctgcccgcg gccgaaggac ccgccgttga cggcccggcc ccctgcagca agcgaccgcc
     2941 gcgcgccgcg gcgtgacgct gcgagctatg cccgcgccct cgtgatgaga cagctttggc
     3001 agacgatccg gagctggttt cgctcgtcgg gctcgcctcc acccattcgt ccagattgcc
     3061 caccatgtcg aaaacagcgt cgtcgccgaa gcggctacgg cacgcagggc tcgagccagt
     3121 caggcgtaac aacggcgccc ccgaaggctc tgacaccaga ttcagtcgcg gatcccgatg
     3181 accatagaaa gcagccccgt ggagggcggt agccggatga acagcccggt agacgttgca
     3241 cgcattggga tcgtagctcg agccgtacgg aaacttcgcc ccgctctggc ccttgcaagc
     3301 tgtcacccac tcattctcgg tgcacaagcg tttagctgca ttttcgcagg ctttcttggc
     3361 gagccaatag gagaggtaac cttgcgggac cacccctgcc tccgagacag cgcgggggac
     3421 gtaatccgac cgtgaccgct gcacccacgg cacttcgggc agtggcattt cgcgggcgga
     3481 gttcgttccg acgcgcagct tctcgagctg ccagccgtcg aagatgctgg ccaccagctt
     3541 ggcgtcaggc gggtaataag gggacagcgc cgagtgcgtg cgggcatcca ccgtgcgaac
     3601 ctcgaagcga tcgatacaga aatctctcac cgctaccatc tctggcgggc agcgccgact
     3661 atgtttcgcg cgcccaggag aagcgccagc gggcccttcg aaaagcggcg actgtgccga
     3721 aatcgcccca gtcgtcggct ggctggggtg aacacggcct ggatcgatac tgccgggatg
     3781 agccgactga cccagtcgag aagagtcgct cgaacgagcg gcatcgtccg cgttaacaga
     3841 gtcgctcggt tgagcgtgta accacgccag aggcgcgagc agggcgggca ataaccagcg
     3901 catgcgggcg cgttgtttac cacctcgcgc cgcgggccaa gaatcgaatt cgaaagcatt
     3961 gccaagctac gggctaggtc ccacgctggg gacgtgccct ggagaaagac tacaggtggg
     4021 cccgccgagg cctcgcgagc gaacgacgcg tcccccgcat ccgagacgcg cgctcgccac
     4081 caactcgtgc aggacatcga agggcacgcc gagatccacg acccgcgaat cctcgccgcg
     4141 ttcgagcggg tgcctcggca cttgttccta ccccaaacgc accgctcgta tgcgtatcga
     4201 gattcggcgc tgcctatcgg cttccaacag acgatcagcc aacccagtat gattgccatc
     4261 atgctcgagg cgttggaact cgacccccag caccgggtgc tcgaagttgg cgccggctcg
     4321 ggctacgcgg ctgcgttact gggcgagctg gtgcgtgaag ttcacagcgt cgagatcgtg
     4381 ccggaattgg ccgaacaggc gcgcgcgcgg ctgcaggccc tggagtaccg gaacatcttc
     4441 gtgcatcacg gcaacggtcg ctccggttgg tgggaccagg cgccttatga ccggattctc
     4501 gtctccgcgg gcgcggagag cgttccaccc gaactcattc atcagctcgt ccccggcggc
     4561 atattggtga tgccgatcgg cgatcagagc gggcaacgac tgctaatcgc gcacaaagat
     4621 gcagccgaag gaatgcactt ttcggagtcc attccctgca tcttcgtgcc gctcgtcagc
     4681 accaattagc aaccgcgctc aagccaggcc gactttggag gagaaggcga tcgggctcgt
     4741 gcccgcgcgc tcgtaggagc gccgccagag ctcgtcgcgt ggcacctcga atacgagtct
     4801 gcggtccaga tcgaccggta gccacgcccc gctacgaatc tcgttctcga gttggcccgg
     4861 cgcccaccca gcgtatcccg cgaccccgaa caacgaagcg ggaggctctc gcagagcaat
     4921 caactcgagc gctcgacgtg acgcacacga caacactccg gctccgacct cgaactgccc
     4981 atccacttcc gagaaccggt cttcttcgcg gtatatcagc caaacttgct ccttcgagac
     5041 cggccccccg agccgcaccg atcccgagcg aggcggcgga tccttcgaga tgtccgcgcg
     5101 cagcagcagc tcggacatgg tcatcacgtc gcgaccattg attatccatc cgaaggcgcc
     5161 gtccgcgccg tgagacgcga gcagcaccac ggctcggtcg aagttgggat cgccaagcgg
     5221 cggagccgcg actagaaagc ctggagcaag cgcagacggg ccaagcgcag acatgggcag
     5281 cccgaagtct atccgaagtc agccgccact tctgccgacc tgcgcatgaa aaccgctacc
     5341 ccaccccgtc ccacccggcc cgaatccctc accggcggag tagtt
//
LOCUS       DASTKU010001061         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_210595, whole genome shotgun sequence.
ACCESSION   DASTKU010001061 DASTKU010000000
VERSION     DASTKU010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210595"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFQ61_37750"
     CDS             <1..261
                     /locus_tag="VFQ61_37750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960315.1"
                     /translation="YCGIDQENVRCEAVLDMTTRAQECPSGDDDACGCERDESGACTS
                     SGQGGLCRDLALVKNRCTYRCGSPDDCPTGKKCDGTPIAYCQ"
     gene            697..1623
                     /locus_tag="VFQ61_37755"
     CDS             697..1623
                     /locus_tag="VFQ61_37755"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET9960316.1"
                     /translation="MFKPETSFAAFACGAGAQLRGESASDASSKAAILKRHLLPFFGI
                     MTLPDVARSVANNEYQRAKASQGLTNKTINNQMVVLRALLRLAAAERLLDDVPAIRGL
                     AVVGSAPRCLNLGEAHRLVSLAEQEWRLMILCALSTGLTLSELRALKWEHVSLEDGVL
                     VSPSPSRKCARRQFPLCPALVEEFRRAHNHKSHVFVRSAGDALTHGQCKWPLARAARA
                     AGIVGLSWRHLRHTCCVQLLSAGTPRAIVQRLLGYQSNAALDRFQGHGLLLGEQPIHV
                     LQDGQSHSAFLCQLEFISKNHAALTSIIFGAA"
     gene            complement(1750..>2201)
                     /locus_tag="VFQ61_37760"
     CDS             complement(1750..>2201)
                     /locus_tag="VFQ61_37760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960317.1"
                     /translation="ADAFASAFLMPRETVRAHVRVGATRPELIAAKHRWGVSLAALAN
                     RSHRVGILSDWHYRGLCIEMGQQGDRTTEPRPITARETSQVLAKVFGALRDEGVTRNA
                     VAADLAIEPSELDSLIFGLMLMSVESEAASEQAPPPRSSPARLRLVQ"
BASE COUNT          420 a          672 c          695 g          414 t
ORIGIN      
        1 tactgcggca tcgatcaaga aaacgttcgc tgcgaggcgg ttctggacat gacgacacgt
       61 gcccaagaat gcccgagtgg agatgacgac gcttgcggct gcgagcgcga cgagagcggg
      121 gcgtgtacat cgtcaggcca aggcggtctt tgccgggacc tcgctctcgt caagaatcgg
      181 tgcacctatc gctgcggttc acctgacgac tgtccaacag gcaagaaatg cgatggaact
      241 ccgatcgcgt actgccaata acccccttgc ctgaacgcgc agctcgtccc gggttggccc
      301 gggcgcagtt gccccgaact aacctcgtgc atcccagctg ctctagtgag ctcgaccggc
      361 gcaccgcgcg ctggttagtc ggtgggcaca cccatccatt gctcgagtga gcaaccggcg
      421 cggcctcgtc gaacaaccca ggtgagcaga gctcgcggcc gttcgtaggg gcctagttgg
      481 ggagtgcgcc acacttggcg ctctcctaca gcgccgatgc cgattgtaaa cgcaacgaag
      541 aagaaggcga gaacttcaca agtcctcacg aggcacccag gtcgagcttc aaatcgtcgc
      601 gcttcaacgt tgggaagcgt tgctctggtg cgtcggcgtt ttgccgatcg ccgaagagtc
      661 gcgcctgacc ctgacgcggg aaggaaagga acgcacgtgt tcaagccaga aaccagcttt
      721 gccgcattcg cgtgcggcgc gggcgcacag ctgcgcggtg agtcagccag tgatgcgagc
      781 tccaaggcag cgattctgaa gcggcatctt ttgccgttct tcggaatcat gacgcttccg
      841 gacgtagcgc gaagcgttgc caacaatgag taccagcggg ccaaggcctc gcaaggcctg
      901 acaaacaaga cgatcaacaa ccagatggtc gtgcttcgag ctctcttgcg actcgcggcg
      961 gctgagcgtc tcctcgacga tgtgcccgcg attcgcggtc tcgcggtcgt ggggagtgcg
     1021 ccgcgctgtc tgaacctcgg tgaagcgcac cggttggttt ccctggcgga gcaggagtgg
     1081 cggctcatga tcttatgcgc tctctcgacc ggcttgaccc tctcggaact ccgcgcgctg
     1141 aagtgggagc atgtcagctt ggaggatggg gtactggtaa gcccgtcccc atccagaaag
     1201 tgcgctcggc gtcagttccc gctctgtccc gcgctcgtgg aggagttcag gcgggcgcac
     1261 aaccacaagt cgcacgtctt tgtgaggtcg gctggcgacg cattgacgca cggacagtgc
     1321 aagtggcctc tagcgcgcgc cgcccgagcc gcggggattg tagggttgag ctggagacac
     1381 cttcgccaca cctgttgcgt gcagttgctt tcggcgggca ccccgcgcgc tattgtgcag
     1441 aggctcctcg gttatcagag taacgctgcg ctcgaccgct tccaaggcca cgggctcctg
     1501 ctcggtgagc agcccatcca cgtcttgcag gacgggcaga gccactcggc gttcctgtgt
     1561 cagctcgagt tcatttccaa gaaccatgcg gcgcttacgt caatcatttt tggggccgcg
     1621 taggatctgc gaacgtcgtg ctctggccga gtgaagcgtg cccaggttca tggagctcgg
     1681 gaacctgcgc cagtcgagta ttgtgtgcca ccgcagtgaa catgatgtgc gcccgagctg
     1741 cggtcacatc tattgcacga gccgcagccg ggctggcgag ctcctaggcg gaggggcttg
     1801 ctccgaggcg gcctcgctct caacagacat gagcatcaag ccaaagatca aggaatctag
     1861 ctccgacggc tcgatggcga gatcggcagc gacagcatta cgggtaacac cttcatcgcg
     1921 tagagctcca aacaccttgg cgagcacctg cgatgtctcc ctcgcggtta tgggtcgcgg
     1981 ctctgtagtg cgatcgccct gctggcccat ctcgatacaa agcccgcggt agtgccaatc
     2041 tgaaagaatc ccgacacggt gagaacgatt ggctagagca gccagcgaaa ctccccatcg
     2101 atgcttggcc gcgatcaact cgggacgggt cgcgccgact ctcacgtggg cacgcaccgt
     2161 ttcgcggggc atgaggaagg ccgatgcaaa ggcgtccgct t
//
LOCUS       DASTKU010001062         7158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_432543, whole genome shotgun sequence.
ACCESSION   DASTKU010001062 DASTKU010000000
VERSION     DASTKU010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432543"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VFQ61_37765"
     CDS             <1..1215
                     /locus_tag="VFQ61_37765"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET9960318.1"
                     /translation="TYDPGFMNTCSCRSSVTYIDGDLGILRYRGYPVDQPAENATYLE
                     VAYLLINGELPTKSELEEFTTNVTHHTFVHENVRTFIDGFRYDAHPMGMLVATVGALS
                     TFYPEAKHVLDHEIRRNQMIRLIAKMPTLAAWSYRHSMGMPFVYPSNELSYTENFLYM
                     MKRIAEPKFEVHPEIVRALDVLFILHADHEQNCSTSVMRGVGSSRADPYVAVAGAAAA
                     LYGPLHGGANEAVLRMLREIGNIGNVPAFIKEVKGGVGGRLMGFGHRVYKNYDPRARV
                     IKRLADQVFEIVGKKSPLLSVALELERIALSDDYFVTRKLYPNVDFYSGLIYEALQIP
                     VDMFPVLFAIPRTAGWLAQWEEMLLDKEQKIARPRQIYTGYDERKFVAIGDRRRSSIN
                     PEATKSSSRETD"
     gene            1314..2357
                     /locus_tag="VFQ61_37770"
     CDS             1314..2357
                     /locus_tag="VFQ61_37770"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9960319.1"
                     /translation="MAASEPPSAQPSEPPREAPRRDSVASFRVPTSSLTGAILDGRYK
                     VHGCVERSAISRTLLAEDLRTHGAVWLRVLTRAGHADHARVASIRERLASLERVQHPN
                     LLEVLGGGVTAGDSPYLVTEAVEGEALEQVLLRQERLSPDICLVLARQAAGALAALHA
                     AGVIHGRLDPSRIVLLGSPNEPYGLKVTDYGTSRLFEPPGSDADSIPLDYRAPEQLTD
                     GTARTQSDVYALGVLLFRLLTFQLPFCGAGPTGSTAESWSRLYSPLPAPAGLESRIDP
                     RIEAVILNATRKRPENRYPSVADLVMDLDALVGLSTHDVTIRSLRVSPDLFEPETQAG
                     TEALRRAQLNRGT"
     gene            2357..4648
                     /locus_tag="VFQ61_37775"
     CDS             2357..4648
                     /locus_tag="VFQ61_37775"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET9960320.1"
                     /translation="MQTWLALVACGVALACTPATTVVDPPVAVRASPPASLPAVQVLV
                     QRSGDPASSLAVDAQAGAPQSGIGQGGRPMTRREHVTDDFFGVRVLDPYRWLEDSKSE
                     EVKSWIEAQNTLTRRTLASYGRQRELHQQIEAQLAIGTLSAPVIRRLPRGGVRIFYTR
                     REGKQEQPVLYIRDGLAGRERMLLDVNALDRAGTTALDYWEPSWDGQKLAYGASFGGS
                     EHSTLYVRDVERDTDLTDRIENTRFGSVVFSPDGKRFFYTRYPSRGSVPAGEEDFHRR
                     VYEHVIGQAPESDRLVFGAELDATAFTMLSASPNGRWLVANVSHGWSQSALYLCDLRA
                     PEPRFTRITPEGENIYQERVRDDAIWVTTNEGAPRYRAFRVDPLQPAREKWRQVVAEH
                     PTDVLQELEPAGDSLYARYGHAGASRLERFTRSGRVRGSIELPSEGSSDGFSVLPDGS
                     LILFGFESFVHPPEIRQLDASTGRSSSWLRVHASVRSGDYTLESRTARSPDGTTVPYR
                     LIRKTGVDLTRSDAPTVLYGYGGFNQNMEPRFSRSLLVFLEHGGVFAQALLRGGGEFG
                     EDWHRAGQGLNKQHTFDDFLAVAEDLTTSHVTRPGRLAIQGRSNGGLLVAAAVTQRPE
                     LFRAAVAGVPLTDMLRYPRFSIGRLWVPEYGSPEKPDEFRALFAYSPYHRVRPTQYPA
                     VLVTTAAGDTRVDPLHARKWVAALQHANLSTHSILLRTEQVAGHGAGTPLSKQVAELT
                     DVYTFLFHELGMTDAPKQDAASD"
     gene            4699..5025
                     /locus_tag="VFQ61_37780"
     CDS             4699..5025
                     /locus_tag="VFQ61_37780"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HET9960321.1"
                     /translation="MTTAKEIMTLLDARLKEREAQAKAVGALYKFVLDGDGGGTFIIN
                     LKDSVGVTEGEGAAPCTIRMSASDAVDLLQGRANGQALFFSQRLKVEGDLALALKLQA
                     LTDILK"
     gene            5022..5705
                     /locus_tag="VFQ61_37785"
     CDS             5022..5705
                     /locus_tag="VFQ61_37785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960322.1"
                     /translation="MSSRGPSDPPVPGQLGTTWPLFTEHPNPSFPENARALVTPFRPP
                     TGHLEALERRAELPPEGSEQYERELNALRASQPPVVPDWMRAARAMIAWLEERVQRGS
                     VDALDEAAIARTWVAFSLGGAVTAHVMRVAHLVSRAHHAIRDSRRVTRDVQAAIRDCA
                     GVLHAGLPLAIRERMPIERTVLVVRRLHEEQDNWAAVVEGTAELLGWKDYARIHAASV
                     IRLVIEHSR"
     gene            5790..6986
                     /gene="pheA"
                     /locus_tag="VFQ61_37790"
     CDS             5790..6986
                     /gene="pheA"
                     /locus_tag="VFQ61_37790"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET9960323.1"
                     /translation="MSTSNSKSSNSPKLSSPRPQGTEPADESAAADAPSLESMRSEID
                     RVDDQLLELLERRADVARRLAALKRSSGLSLRDADREARLLARLEGLADAHGGLFPKS
                     SIRPIFREIISACLSLQEPQTVAYLGPPGTFSHIAARAAFGLGAKYVELPSIGQVVDA
                     VARGAVSYGLAPIENSTEGAVGATLAALLECDVMIERELVIPVSACLLAQSTDLSSIR
                     RVYSHPQPLAQCRNWLAQNLPHAELVSSASTAAAAGEARVDETAAAVGSHLSAELYDL
                     KVVRENIQDRPENATRFCVLSKQDAPRSGRDKTSLVFSTPHARGALRRALTIFDDEGL
                     NLTRIESRPALGQRWQYVFFTDVEGHRSDAEVSRAIERLRTDCSTVKVLGSYPRCDAP
                     ENLGTS"
BASE COUNT         1272 a         2357 c         2261 g         1268 t
ORIGIN      
        1 acgtacgacc cggggttcat gaatacgtgt tcctgccgat cgtcggtcac gtacatcgac
       61 ggcgatctcg ggattttgcg ttaccgcggc tatcccgtgg atcagccggc agagaacgcc
      121 acctatttgg aggtcgcgta cctgctcatc aacggtgagc tcccgaccaa gagcgagctc
      181 gaagagttca ccacgaacgt gactcaccac acgttcgtgc acgagaacgt gcgcaccttc
      241 atcgatggct tccgctacga cgcgcatccg atgggcatgc tggtggccac ggtcggagcg
      301 ctcagcacct tctatcccga agccaagcac gtgctggatc acgagattcg gcgcaatcag
      361 atgatccgct tgatcgccaa gatgccgacg ctggcggcat ggagctaccg ccacagcatg
      421 ggcatgccct tcgtgtatcc gagcaacgag ctcagctaca cggagaactt cctgtacatg
      481 atgaagcgca tcgcggagcc caagttcgag gtgcatcccg agattgtgcg tgccctcgac
      541 gtgctcttca tcctgcacgc agatcacgag cagaactgct ccacgagcgt gatgcgcggc
      601 gtgggtagct cgcgcgccga tccgtacgtg gcagtggccg gcgcggcggc ggccctgtac
      661 ggcccactgc acggcggcgc caacgaagcc gtgctgcgca tgctgcgcga aatcggcaac
      721 atcgggaacg tgccggcctt catcaaagag gtcaaaggtg gcgtgggcgg tcgcttgatg
      781 ggcttcggtc accgcgtgta caagaactac gatccgcgcg cgcgagtgat caagcgcctg
      841 gccgatcagg tgttcgagat cgtggggaag aagagcccgc tgctttcggt agccctcgag
      901 ctcgagcgca tcgcgctctc ggacgactac ttcgtcacgc gcaagctgta cccgaacgtc
      961 gatttctact ccggtctgat ctacgaggcg ctccagatcc cggtggacat gttcccggtg
     1021 ctgttcgcga tcccccgcac cgcgggttgg ctcgcgcagt gggaagaaat gctgctcgac
     1081 aaagagcaga agatcgcacg gccgcgccag atctacaccg gctatgacga gcgcaaattc
     1141 gtcgcgatcg gggatcgtcg gcgcagcagc atcaatcccg aggcgaccaa gagctcctcc
     1201 cgcgagacgg actgagccga aggctctgac tgccccaaca ggccgggccg tcgcagcatc
     1261 caacctgctg caacgcgccc ggtctcgttt tattcgattg ctgttccccg gccatggctg
     1321 cgagcgagcc tccttccgcc caaccttccg agccgccgcg tgaggcgccg cggcgtgact
     1381 cggtggcgtc cttccgtgtg cccacgtctt cgctgaccgg cgcgatcctc gacgggcgtt
     1441 acaaggtgca tggctgcgtg gaacgaagcg cgatcagccg cacgctgctg gcggaggatc
     1501 tacgaaccca tggcgccgtc tggctgcgcg tgctgacccg ggcgggacac gcggatcatg
     1561 cgcgcgttgc gagcatccgc gagcggctcg cgagtctcga gcgcgtgcag catccgaatc
     1621 tgctcgaggt gttgggcggc ggggtcaccg ccggcgattc accctatttg gtgacggagg
     1681 cggtcgaagg ggaagccttg gagcaggtgc tccttcgaca agagcggttg tcgcccgaca
     1741 tttgcctcgt gctcgcgcgc caggcggccg gtgcgctcgc cgcgttgcac gcagcaggcg
     1801 tgattcacgg gcgactcgat ccgagccgaa tcgtgctgct gggatcgccg aacgagcctt
     1861 atggtctgaa ggtcaccgac tacggcacga gccgtctatt cgagccgccc ggctcggacg
     1921 ctgattcgat cccgctcgac taccgagccc cggagcaact cacggacgga acggcacgaa
     1981 cgcaaagcga cgtctacgcg ctcggcgtgc tcctgttccg cttactcacc ttccagctgc
     2041 cgttctgcgg ggcgggtccg accggttcaa ctgctgagtc ttggtcgagg ctctattctc
     2101 ccctgcccgc acccgcgggc ctggagagcc gcatcgatcc tcgaatcgaa gcggtgattc
     2161 tgaatgccac gcgcaagcgc cccgagaacc ggtacccgag cgtcgcagac ttggtcatgg
     2221 acctcgacgc cttggtaggt ctctccacac atgacgtcac gatccgcagc ctgcgcgtaa
     2281 gcccagatct tttcgaaccc gagacgcaag cgggaacaga agcgctgcgg cgcgcgcagc
     2341 tcaacagagg aacctaatgc aaacgtggct cgcgctcgtg gcttgtgggg tcgccctggc
     2401 gtgcacgcca gcgaccacgg tagtagatcc gcccgtcgcc gtccgcgcgt ccccgccagc
     2461 gtctttgcct gcggttcagg tgttggtcca gcggagcggt gatccggcga gcagtctcgc
     2521 cgtcgacgcg caggcgggcg cgcctcaatc gggaataggc caggggggca ggcccatgac
     2581 gcgccgggag cacgtcacgg acgacttctt cggggtccga gttctggatc catatcgctg
     2641 gctggaggac tcgaagagtg aagaggtaaa gtcctggatc gaggcgcaga acacgctcac
     2701 tcgacgcacg ttggcaagct acggccggca gcgagagctg caccaacaga tcgaagcgca
     2761 gctcgcgatt ggaaccctgt cggcgccggt catccgccgt ttgccccggg gtggagttcg
     2821 catcttctac acccgccgcg aaggcaagca agagcagccc gtgctctaca tacgagacgg
     2881 gctggcgggg cgtgagcgga tgctactgga cgtgaacgcc ttggatcgcg cgggcactac
     2941 ggcgttggat tactgggagc cctcgtggga cggacagaag ctggcctatg gcgcgagctt
     3001 cggaggctcc gagcacagca cgctgtacgt gcgagacgtc gaacgcgata ccgacctaac
     3061 cgatcgaatc gaaaacacgc gcttcggctc ggtggtattt tcccctgacg gcaagcgctt
     3121 cttctacacc cgctacccgt ccaggggcag cgttcccgcg ggagaggagg atttccatcg
     3181 ccgcgtctac gaacacgtga tcggccaggc gccagagagt gatcggctgg tgtttggcgc
     3241 cgagctcgac gccaccgcgt tcacgatgtt gtccgcgtct cccaacggtc gctggctggt
     3301 cgcaaacgtg tctcacggct ggagccaaag cgctctttac ttgtgtgact tgcgcgcgcc
     3361 cgagccccgc tttactcgca tcactcccga gggagagaac atctaccagg agcgcgttcg
     3421 tgacgacgcg atctgggtga ccacgaacga aggtgcccct cggtatcgag cctttcgggt
     3481 cgaccccctg cagcccgccc gggagaagtg gcgccaggta gtcgcggaac accccacgga
     3541 tgtgctccaa gagctcgaac ccgcaggaga cagcctgtac gcgcggtacg gccacgccgg
     3601 cgcatcgcgg ctcgaacgct tcactcggag tggtcgagtg cgcggttcca tcgagctgcc
     3661 tagcgaaggt tcgagcgacg gtttctcggt tctacccgac ggctcgctga tcttgtttgg
     3721 tttcgagtcc ttcgtgcatc cgccagaaat ccgacagctc gacgcgagta ccgggcgaag
     3781 ctcgtcgtgg cttcgcgtcc acgcctcggt tcggagcgga gactacacgt tggagagccg
     3841 caccgcgcga tctcccgacg gcaccacggt gccataccgc ttaatccgta agaccggcgt
     3901 cgatctcacg cgcagcgatg cccccacggt gttgtacggt tatggcggct tcaaccagaa
     3961 catggagccg cgcttctccc gttctctgct cgtgttcctc gagcacggcg gggttttcgc
     4021 gcaggcgttg ttgcgaggcg gcggagaatt cggcgaggac tggcaccgcg ctgggcaggg
     4081 tttgaacaag cagcacacat tcgatgactt cctggccgtc gccgaagacc tgacgaccag
     4141 ccacgtcacg cgtccgggcc ggcttgcgat tcagggccgt tcgaacgggg gattgctggt
     4201 agcggctgcg gttacccagc ggccggagct gtttcgcgct gctgtggccg gcgtgccgct
     4261 cacggacatg ctgaggtacc cgcgattctc cattggtcgc ctctgggtac cagagtacgg
     4321 ctcccccgaa aaaccagacg aattccgtgc gctgttcgcg tattcgccct accaccgcgt
     4381 gcgcccgacc cagtaccccg ccgtcctggt caccacggcc gctggagata cgcgcgtgga
     4441 tccgcttcac gctcgcaaat gggtcgccgc acttcaacac gccaacttgt ccacgcactc
     4501 gatcctgctc cgcaccgagc aagtggcggg tcacggtgcc gggacgcccc tcagcaagca
     4561 ggtggccgaa ctgaccgacg tatatacctt cctttttcac gaactcggta tgaccgatgc
     4621 tccgaagcag gacgcggcct cggattgagg gtacgcgctg tactaatacg ccagaccttg
     4681 tatcctccta gaactcgcat gaccaccgcc aaagagatca tgaccctgtt ggacgctcgc
     4741 ctgaaggagc gtgaagccca agccaaggcc gtaggtgccc tctacaagtt cgtgttggac
     4801 ggagacggtg gtggcacctt catcatcaac ctgaaagaca gcgtcggggt gaccgaaggc
     4861 gaaggcgccg cgccgtgcac catccgcatg agcgcttcgg acgccgtcga cttgcttcag
     4921 ggccgcgcca acgggcaggc gttgttcttc tctcaacgct tgaaggtgga aggagatctg
     4981 gcgctcgcgc tcaagctgca agctttgacg gacattttga agtgagctca cgcggccctt
     5041 cagatccgcc tgtgcccggc caactcggca cgacctggcc gctcttcacc gagcatccca
     5101 atccgtcttt tccggagaac gcgcgggcgc tcgtcacacc tttccgcccc ccaaccgggc
     5161 acctcgaggc gctggaaagg cgcgcggagc tcccgccgga gggcagcgaa caatacgaac
     5221 gcgagctgaa tgccctacgc gcgtcccagc ctccggtcgt gccggactgg atgcgggcag
     5281 cccgggcgat gatcgcctgg ctcgaggagc gtgtgcagcg gggcagcgtg gacgcgctgg
     5341 acgaggcggc gatcgcacgc acctgggtcg cattctcgct cggcggcgcc gttaccgctc
     5401 atgtgatgcg agtggctcac ctggtgagtc gcgcgcacca tgcgattcgc gatagccgcc
     5461 gcgtcacgcg cgacgtccaa gccgcgattc gcgactgcgc gggtgtgctg cacgcaggcc
     5521 tcccactcgc gattcgggaa cgcatgccga tcgagcgcac ggtgttggtg gtgcgccggc
     5581 tccacgaaga acaggacaat tgggccgctg tggtcgaagg cacggccgaa ttgctgggtt
     5641 ggaaagacta cgcgcgcatt cacgccgcgt ccgtcattcg cctcgtaatc gaacacagtc
     5701 gctgaatcgg ctttccgctc ccctcctcgg ctcgcgaccg agcgaatgat agaacccgag
     5761 ccgcgccgac cgctccaggc atgatgaaga tgtccacgtc caactcgaaa tcatcgaata
     5821 gccccaagct ctcgtccccg cgtccccaag gcaccgagcc agccgacgag agcgcggccg
     5881 ctgacgctcc gtcgctcgag agcatgcgca gcgagatcga ccgtgtcgat gatcagctgc
     5941 tcgaattgtt ggagcgtcga gccgatgtgg cacgtcgctt ggccgccctc aagcgatctt
     6001 caggcctctc gctgagggac gcggaccgcg aagcgcggct cttggcgcgg ttggagggcc
     6061 tcgccgacgc ccacggcggg ttgttcccca aatcgagcat tcggccaatc ttcagagaaa
     6121 tcatcagcgc gtgtttgtcg ctccaagagc cgcagacggt cgcttacctt gggccacccg
     6181 ggactttttc gcacatcgcg gcgcgcgcgg cgttcgggct cggcgccaag tacgtcgagc
     6241 taccgagtat cgggcaagtc gtcgacgcgg tggcccgagg cgccgtgagc tacgggctag
     6301 cgcccatcga aaattcgacg gagggggcgg ttggcgcgac tctggcggcg ctgctcgagt
     6361 gcgacgtgat gatcgaacgt gagctcgtta tccccgtgag cgcctgctta ctcgcgcaaa
     6421 gcactgactt gtccagtatc cggcgggttt actcacaccc gcaaccgctc gcgcaatgcc
     6481 ggaactggct cgcccagaac ctgcctcacg ccgagctggt cagctccgcc tcgaccgcgg
     6541 ccgccgccgg agaggcccgc gtcgacgaga ccgcagccgc ggtcggctct cacctttccg
     6601 cggagctcta cgacctcaaa gtggtgcgtg aaaacatcca ggaccggccc gagaatgcaa
     6661 ctcgcttttg tgtgctctcg aagcaggacg ccccccgcag cggccgggac aagaccagtc
     6721 tcgtgttctc gaccccgcac gcccggggag ccctgcggcg cgccctcacc atcttcgatg
     6781 acgagggcct gaatttgacc cgcatcgagt ctcgaccggc gctaggtcaa cgctggcaat
     6841 acgtgttttt caccgacgtg gaaggccacc gctccgacgc agaggtatcc cgcgccatag
     6901 agcggctgcg cacggactgc tccaccgtca aagtgctcgg cagctacccg cgctgcgacg
     6961 ccccggaaaa tctgggcacg agctaggagc atcccgcgag ttctccgcgt ctcccgagct
     7021 tcggcatttg cgtgccgagg cgcacggcaa tcgtgcgtgc aagcggtgag cagtgctaat
     7081 ccccagagca tgtggttccg taagctggtc ggctatttcc tccgaggtgc tctgctcacg
     7141 gcgcccctgc ttcttacg
//
LOCUS       DASTKU010001063        10704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1998999, whole genome shotgun sequence.
ACCESSION   DASTKU010001063 DASTKU010000000
VERSION     DASTKU010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10704
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998999"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VFQ61_37795"
     CDS             complement(<1..854)
                     /locus_tag="VFQ61_37795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9960324.1"
                     /translation="MASKNDPEVARYIANVHSFAQLSREDELSLLTRWLETRDEAART
                     RVVKAHLRYVVAIALRYRRYGLPLADLISEGNFGVVHALDKFEPARGTRFVTYAAYWI
                     RAYMLNHIIRSWSLVGVGSGALRSKMFFKLRRERVRITNLVGEGDHADDMLAEAMALP
                     KSKVVSMVRRLEARDLSLDAKVFDDSASTLADTLHSPEASQEEHLGANQVGHFLRDVV
                     SSALEVLDHRERYIVEKRLMADAEDELSLAEIGRRLGVSRERARQLEARAKRKLKNRM
                     ADLGSVGS"
     gene            complement(1217..1540)
                     /locus_tag="VFQ61_37800"
     CDS             complement(1217..1540)
                     /locus_tag="VFQ61_37800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET9960325.1"
                     /translation="MISITTLAAEKVREISSSEGLEGQGLRLRVIGGGCSGFQYDLYF
                     EEAPTELDEQFESHGVKLYVDPLSFQYLDGTEIDYVETAHASGFKFGNPNVTGTCGCG
                     SSFKV"
     gene            2038..2787
                     /locus_tag="VFQ61_37805"
     CDS             2038..2787
                     /locus_tag="VFQ61_37805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960326.1"
                     /translation="MGEVSPPPPELLLKPDGDEAPDDGIQGEAPAPPPPPPRREAKEA
                     KASAPPKAPPAPSQTKSAAFVHDGFYLRFALGPALSATSLRTDRVASPDVSLTGFGGA
                     LDAWLGWTVASGVVLGPSLSGASHQSSAARLGETKGQIDAARTLFGIFLDAYPNPQRG
                     FHFGGILALANLQLSGKDGGNFSSFSGGGLGLEVFGGYEIFIARDWSAGGMVRLGGVF
                     VRDEESFGTNDQVTRQATSFSAALLATLVYH"
     gene            complement(2841..4013)
                     /locus_tag="VFQ61_37810"
     CDS             complement(2841..4013)
                     /locus_tag="VFQ61_37810"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9960327.1"
                     /translation="MSRSSRLRWGAATCGLVASLFAPLVHSQVDTLRAQHFALATARL
                     PQRTSPGPLPQTPRVGLRVRVAGGILHPPTVAPDDTLLLALSNPVIAAYEVQRGQLLW
                     TARLGSSPAASSVIALRDGRRAVITENLELIVFNRLGRTLSRRTLPLSGADPRLRVSA
                     APDGGLWIVLGRRVLRLDAALELRFQTQIATEAAVVLPDQSPPLLVAATGEVYAVATS
                     GEVTRVGNFQGRVEDAVAADELHVLAVVDGKRVLELDLESGGLSPRFLEPDLDLQPRL
                     TRNSSGLWFTTSNDLLLSLTNSGRERLRAPIPASPFTRLKTVDVLPGPDGTAFVSRSA
                     TDLLHIAADGNVSRVEGTACAEPLPPQPLPGSRVILACRSGIILRLDDAATSARAP"
     gene            complement(4000..6057)
                     /locus_tag="VFQ61_37815"
     CDS             complement(4000..6057)
                     /locus_tag="VFQ61_37815"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9960328.1"
                     /translation="MTSASRSEPQVCPECHALCGGTARYCAECGSPLSTKSAVKPRTA
                     AERESHLADPLIGRVIADRYRITEEIGRGGMGVVYRVEHARIGKLMALKLLSGELTRD
                     PLQIARFKREAQMASQLSHPNTVQVFDFGISDGLVYLAMEYLRGEDLGHIVRREGSLS
                     VERTTKLMIQVSGSLAEAHGKGIIHRDLKPENILVVPGQATEDLVKVLDFGLAKLRES
                     NEPLDVTLRGAIVGTPYYMSPEQIRGENVGQESDIYALGALMYMCLTGVPVFDAQTAV
                     GVFTKHLLEAPIPPHVRKPELGIPEGVSALVVHALAKDPQARPRSAIELRNALLEIVS
                     GRISVEKLISEGELRQLRHDTEAATRDEVERYERKLRRRGRLAWAAVAVGVLGVLGVV
                     GMLWFGIRWPSAYEGREREPNDLASQASDLRFGSKLLGKIGRRIDLERSDRDFFRVDV
                     PASITAVRLSTRALPNFATCTWLYAAGAESPLGRYCSGAAGRDLVVAQLAIAPGSYLL
                     AVMQDRDAYTRTGAPPVLENVSDDYELELGGVVARSNHELEPNDSERNPNILKRGSPL
                     RGSFAFMRDVDVFCAHEVGSLRFVIADLPDRPRSRDAVLEVTPLSGPTRDVPVRVHAA
                     NATFPSSPRDIQTPFRGPWIVGGREQADCVQLTLRPNPWSPNPPEVAPASDSEYEVTL
                     ESQ"
     gene            complement(6054..7973)
                     /locus_tag="VFQ61_37820"
     CDS             complement(6054..7973)
                     /locus_tag="VFQ61_37820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960329.1"
                     /translation="MQTCARCGTENTDAAFSCVTCGLPLSAAENAVIEAPVAGGARSA
                     GGQPNSGSAKEPRQELARTVAGHLDPQLVGTVRVAPDASARPPMAATIIGLAPTPGPE
                     AVEDRPSNATPHFARTVLGLAPPDMRGGTVPLAALSGNPDPNPSAVTPAQPASFENET
                     PQVAEAAAPPGGPMMKTLLGVARPGIAPMNPGVPKQEPAVFPVGLPTPIGAPPLVFPN
                     ESPPNEVFPGGPRDSREFQPVASARKGRWLGLFALFGALTLAGAGVGAFLWLRGPGRI
                     EARAVVDAEGRESLNLTCAGCKDGTRVRLGQELGTIKQGRALVRLGRKLSVGENRFEL
                     FVRAPGSDRETRVALNVPLEYRVQADTRGLEKARPALSVFVEALPGSSVAVDGKAVAL
                     NALGQATYEVDITPELSGLDNMARRLDRKVGYRINVPGGGVASGEVAFQLPIMPLTLE
                     SPGPAITIEGERFLLAGRTSKGAKVTIADRPITIDAEGQFVQPLSVSSVGETRVTLRT
                     SAPDWAPRLFSIHVKRVASLADEGRARRTQSVAQYAALLTDLPKKVGVKVALDGVIAE
                     VGNQPRSTLVLLDVASGCPARPCFARVVHGAPSTLAPGDKVSVFGVIHGSVEGPRAGS
                     PIPEVFADFILKANK"
     gene            8409..9521
                     /locus_tag="VFQ61_37825"
     CDS             8409..9521
                     /locus_tag="VFQ61_37825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960330.1"
                     /translation="MSRHRGSEGSAGLAALGAELENALIPLNRSAARRALCRLARETT
                     RALGAGLNTVQPAPPASKASSSESRVSAPLPGPIPAPVQPVAPDGALDAPLNPGDSAV
                     NEKGRGVERDSARRGVQIALVRRSPTESSPQTPRLGTRIDATADELSDDITQRMPNVQ
                     TVPAARASFTETRVPSRHLDTSDNAHPLERGTRPPGEQTPFWGVPAVHLSPREAGANK
                     APKPSATPSVGAKNKPQFTPGPDALAASYAPPLSPEEEDELEIEILPEPLELSPCRVP
                     PVEPMSHECSAEPSPDSLDVTPVAHHKLVEALHACTTYAGQGAQRSDVRELIADFQVH
                     ADWTEDELSRELKGLAQVELTPAGANAGPRAKSSRR"
     gene            9555..9944
                     /locus_tag="VFQ61_37830"
     CDS             9555..9944
                     /locus_tag="VFQ61_37830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960331.1"
                     /translation="MHDQNLAILKGLVCVAWADGAMHATERQLLEALLQAFNAKPSEA
                     LEIRLFAEQPRTLQDVPIHDLSYADRRMLLTQAVILSFADGEQHSTEQVIIKDLAREL
                     RIPDIEARGIIAAAEEQARQSLELLAK"
     gene            complement(9960..10337)
                     /locus_tag="VFQ61_37835"
     CDS             complement(9960..10337)
                     /locus_tag="VFQ61_37835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9960332.1"
                     /translation="MKILVCEDQDSIRKMIEALVQARGHEVMAVSSGVKALDVAATSP
                     PDLVLLDLMLPGPFDGFEVCRRLRSDPTTQAIPVVIISALDDDDSRARATQAGAAAYY
                     TKPFSPIALLKEIERLRVAPRPG"
     gene            complement(10495..>10704)
                     /locus_tag="VFQ61_37840"
     CDS             complement(10495..>10704)
                     /locus_tag="VFQ61_37840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960333.1"
                     /translation="EDRGPPVPLELWESVVRHDTDPASVGRPLGITLPGAHAALRAIG
                     TELRLREGTLGATELWFTLHGASAS"
BASE COUNT         1958 a         3377 c         3447 g         1922 t
ORIGIN      
        1 aagcttccga cgctccccaa atccgccatt cggttcttca gcttgcgctt ggcgcgcgcc
       61 tcgagttgac gggcgcgctc ccgggacacg ccgagccgtc gcccgatctc ggcgagcgag
      121 agttcgtcct ccgcgtccgc catcaagcgc ttctcgacga tgtaacgctc gcggtgatcc
      181 aagacctcga gcgcgctact cactacatcc cgcaggaaat gcccgacctg attggccccc
      241 aagtgctctt cttggcttgc ctccggagag tggagggtgt cggcgagagt cgaggcggag
      301 tcgtcgaaca ctttggcgtc gagcgacaag tcgcgagcct ccaggcgacg caccatcgag
      361 acgactttcg atttgggcaa cgccatagcc tcggccagca tatcgtcggc gtggtcgcct
      421 tcacctacga ggttggtgat ccgaacccgc tcccggcgca acttgaagaa cattttcgat
      481 cggagcgcgc ctgaaccgac gcccaccaag ctccacgatc gaatgatgtg gttcagcatg
      541 tacgctcgaa tccaatacgc cgcgtaagtc acgaagcgcg ttccgcgcgc cggctcgaac
      601 ttgtcgaggg cgtggactac gccgaagttt ccttctgaga tcaggtcagc cagcggtaag
      661 ccgtaccggc ggtagcgcag cgctattgca acgacatatc gcaagtgcgc tttgacgact
      721 cgcgttcgcg cagcctcgtc gcgcgtttcg agccaccgcg tgagcaggga cagttcgtct
      781 tcgcgggaaa gctgagcaaa ggagtggacg tttgcgatgt agcgagcgac ttccgggtcg
      841 ttcttgctgg ccatgtcggg gttccttctt gtctgtccat tatttgtcct agacagatat
      901 tagtcaaatg ctgtcttgga caaaagttcg acgaagccgg tctaacgttg gtcaactcct
      961 ctacgtgtaa gtggattgcc aagttgcggc ttgagcagca atcggacgaa tggaagtcgc
     1021 atctggactc ttgggcatac cgcagcgatt tgacccagtg acgaccacga gacgcggaag
     1081 ttggggtcat cggggaggac aaacgcaaag cgcccagctt cgctagaagc caggcgctct
     1141 gtttatccag gatgttctct aattcagctc agttgggccg ttgagcggac aaccggctac
     1201 gcaggtgcgc aagactctac actttgaagg aggacccgca gccgcaagtt ccggtgacgt
     1261 tggggttccc gaacttgaaa cccgaggcgt gcgccgtttc cacgtagtcg atctccgtgc
     1321 cgtccaagta ctggaaactc aacgggtcca cgtacagttt cactccgtgc gactcgaact
     1381 gctcgtccaa ctccgtcggg gcctcctcga aatagaggtc gtactggaag ccagagcagc
     1441 cgccgccaat cactcggagc ctgagcccct ggccttccag gccttcggaa gacgagattt
     1501 cacggacttt ttcggctgct agcgtcgtga tcgagatcat cggataacac tacctttcta
     1561 actttgccct tttagctggc ctcgagtgtc tttgcaccac aaggtccttc aaaaatctca
     1621 gctctgcacc tcgagcagcc agttacacgg ttgaaacgct cgagaagaaa ccgttgcgct
     1681 tggaacgtgg aagagaacct tcgtacgagg agggttcttg taaagggctc caatggccgc
     1741 gactcggtca aaatcgacgg aatgcgattc ggcctcagtc gacccctcga ggacgagaaa
     1801 cgcgagggac cagatgcgat ggggcagcga gtcacatcaa cttccgtagt ataggcggct
     1861 tcgaaaggct tgccagtggt ccggcccgtc catgtttcgc gtttcccacc gttggctaga
     1921 gcggccgttg atgctcggcg cctccgcgta aagcgtttgc tttgcagcac ggcgcgcgga
     1981 gcggcgtggg ctggttgcct gttgtacgcg gggagcctgc gtgccgagga gccggcggtg
     2041 ggcgaggtga gcccaccgcc tccggagctc ttgttgaagc cggacggcga cgaagcgccc
     2101 gacgatggaa tccaaggaga ggctccggcg ccgccaccgc ctccaccacg ccgtgaagcg
     2161 aaagaagcga aagcaagtgc gccgccgaag gctccgcccg cgccgagcca gaccaagagc
     2221 gctgccttcg tgcacgacgg cttctatctg cgtttcgccc ttggacccgc gttgagcgcg
     2281 acctcgctac gcaccgatcg ggtggcgagc cctgacgtct ctttgactgg gttcggtggc
     2341 gcactcgacg cttggctggg ttggaccgtg gcaagtgggg tagtgctcgg cccctcgctg
     2401 agcggggcca gccatcaatc gagcgcagct cggctcgggg aaaccaaagg acagatcgat
     2461 gccgcgcgaa cgttgttcgg aatcttcctg gacgcctatc cgaacccgca gcggggcttc
     2521 cacttcggtg ggatcttggc gttggctaat ttgcaactaa gtggcaaaga tgggggtaac
     2581 ttcagctcgt tttccggggg cggcctcggg ctggaggtct tcggcgggta cgagattttc
     2641 atcgcaagag actggtccgc cggggggatg gtgcgcttgg gaggagtctt cgttcgcgac
     2701 gaagagagct tcggaacgaa cgaccaagta acccgtcagg ccacctcgtt cagtgccgcg
     2761 ctgctcgcga cgctcgtcta tcactgaggg tagaatcacc gagcgcgtcg attcaggcgg
     2821 aacctctcct cggggctccc tcatggggct cgcgcggaag ttgcggcatc atcgagtcga
     2881 agaatgatgc ctgaccggca agccaaaatt actcgggatc cgggtaaggg ctgggggggc
     2941 agtggctcgg cgcaagcggt cccctcgacg cgggagacat tcccgtccgc cgcgatatgt
     3001 aagagatccg tcgccgaccg cgagacgaaa gcggtgccgt cgggaccggg taaaacgtcc
     3061 actgtcttca agcgcgtgaa cggggacgcg ggaatgggtg cgcgaagccg ctcgcggccg
     3121 ctgttcgtca gcgagagcaa caagtcgttg ctcgtagtga accaaaggcc gctcgagttt
     3181 cgcgtcaagc gtggctgcag atcgagatcc ggctcgagga agcgaggcga gagaccgcca
     3241 ctttcgagat cgagctcgag cacgcgcttg ccgtccacga cagcgagcac gtgcagctca
     3301 tctgctgcca ccgcgtcctc gacgcggccc tgaaaattgc cgacccgcgt cacctctccg
     3361 ctcgtggcga cggcgtagac ctcgccggtc gcggcaacca gaagcggggg cgattgatcc
     3421 ggaagcacca ctgcggcctc ggtggcaatc tgggtttgaa atcgtagttc gagcgcggca
     3481 tcgagccgta agacgcggcg cccgagcacg atccacaacc cgccgtctgg agcggcgctg
     3541 acgcgcagcc ggggatcggc cccggacaat gggagggttc gacgactcaa cgtgcgtccg
     3601 aggcgattga agacgatcag ctcgaggttc tccgtaatga cggcgcgtcg accatcgcgc
     3661 aaagcgatca cgctgcttgc cgcagggctc gaccccaaac gcgcagtcca gagcagctgg
     3721 ccacgctgaa cttcgtaggc cgcaatcacg ggattggaga gcgcgagcag cagcgtatcg
     3781 tctggggcga cggtgggtgg gtgcagaata ccgcccgcca cccgcacgcg cagcccaaca
     3841 cgaggcgttt gcgggagggg gccggggctg gtgcgttgag gcaatcgtgc ggttgcgagt
     3901 gcgaaatgct gggcgcgcag cgtgtccacc tgcgagtgca ccaagggcgc aaacagggac
     3961 gcgacgagtc cgcacgttgc ggcgccccag cgcagccgac tactgcgact caagggtcac
     4021 ctcgtattcg ctgtcacttg ccggggcaac ttcgggagga ttgggtgacc agggattggg
     4081 tcgcagggtg agctgcacgc aatccgcctg ttcccggccg cccacgatcc aaggaccgcg
     4141 gaacggggtc tggatgtcgc gaggggaaga tggaaaggtc gcgttggccg cgtgaacgcg
     4201 cacgggaacg tctcgcgtgg gccccgagag cggagtcacc tcgagcaccg cgtctcggga
     4261 cctagggcga tcgggcaggt ccgcgataac gaaacggagc gatcccactt cgtgcgcaca
     4321 gaagacgtcc acgtcccgca tgaaagcgaa gctgccgcgg aggggtgaac cacgtttcaa
     4381 tatgttgggg ttgcgctcgg agtcgttcgg ctcgagttcg tgattggagc gcgccactac
     4441 gccgcccaac tcgagctcgt agtcgtccga tacgttctcg agcacggggg gagcgccggt
     4501 gcgcgtataa gcgtctcgat cttgcatcac cgcgagtaga taactgcccg gtgctatcgc
     4561 gagctgtgcg accacgaggt cacgtccagc cgcgccagag cagtagcggc ccaaagggga
     4621 ctcggcgccc gcggcgtaga gccaagtgca cgttgcaaaa ttcggcagcg cccgcgtaga
     4681 caggcgcacc gcggtgatgg aagcgggcac gtcgacgcgg aagaagtcgc gatcgctgcg
     4741 ctcgaggtcg atccgacgcc caatcttgcc gagcagcttg ctcccgaacc gcaggtctga
     4801 ggcctggctc gcgaggtcgt tgggttcccg ctcacgaccc tcgtaggccg agggccagcg
     4861 aatgccgaac caaagcatgc ccaccacgcc gagtacgccg agtacgccga ccgcgacggc
     4921 tgcccacgcc agtcgccctc gtctgcgcag ttttcgctcg tagcgctcca cctcgtcgcg
     4981 tgtggcggcc tccgtgtcat gtcgcagctg gcgcagctcg ccctcggaga tcagcttctc
     5041 gacgctgatg cggccgctca cgatttcgag caatgcgttt cgcaactcga tcgcgctgcg
     5101 cggccgcgcc tgaggatcct tggcgagcgc gtgcacgacg agcgcgctga caccctcggg
     5161 gatgccgagc tctggcttac gtacgtgcgg tggaatcgga gcttcgagta ggtgcttggt
     5221 gaacaccccg accgccgtct gcgcgtcgaa tacgggtacg cccgtcaaac acatgtacat
     5281 cagcgcgccg agcgcataga tatccgattc ttggcccacg ttctcgccgc ggatctgctc
     5341 cggcgacatg tagtacggag tgccgacgat tgcaccgcgc agcgtaacgt ccaacggctc
     5401 gttggattct cgcagcttgg cgaggccaaa atccaagacc ttgaccagat cttccgtcgc
     5461 ttgtccgggc accaccagaa tgttctcggg tttgaggtcc cggtgaatga tgcccttacc
     5521 gtgcgcctca gccagcgaac cgctcacctg gatcatgagc ttggtggtgc gttccacgct
     5581 caacgagccc tcgcgtcgga cgatgtgacc caggtcctcg ccgcgcagat actccatcgc
     5641 gaggtaaacc agcccgtccg agatcccgaa gtcgaacact tgaaccgtgt tcggatggga
     5701 cagctggctg gccatctggg cctcgcgctt gaatcgcgca atctgcagcg ggtctcgtgt
     5761 gagttcaccg ctcaggagct tgagcgccat gagcttgccg atgcgcgcgt gctcgacgcg
     5821 gtacaccact cccatgcccc cgcgtcctat ttcttcggtg attcgatagc gatcggcgat
     5881 cacgcggcca atcaagggat cggcgaggtg actctctcgc tccgccgcgg tgcggggttt
     5941 gaccgctgac ttcgtgctca gaggggagcc acactccgcg cagtaccgag ccgttccacc
     6001 gcagagtgcg tggcactccg ggcaaacttg cggctcggac cgcgaggccg aggtcacttg
     6061 ttggccttca gaatgaaatc tgcaaaaact tctgggatcg ggctgccggc gcgaggaccc
     6121 tcgacgctcc cgtgaatgac accgaacacg ctgaccttgt cccccggcgc gagggtgctc
     6181 ggcgctccgt ggaccacccg cgcgaagcag ggccgcgcgg ggcatccgct cgctacgtcc
     6241 aggagtacga gcgtagagcg aggttgattg ccaacttcgg ctatcacccc gtccagcgcg
     6301 accttcacgc ccaccttctt gggtaaatcc gtgagcaacg cggcgtattg cgcgaccgac
     6361 tgggttcggc gcgcgcgacc ctcgtcggcc aggctcgcga ctcgcttgac atggattgag
     6421 aacagccgcg gcgcccaatc cggcgcgctc gttcggagcg tgacccgcgt ctcgccgacg
     6481 gatgagacac tcagcggctg aacgaactga ccctcggcat cgatcgtgat tggtcgatcc
     6541 gcaatcgtca ccttcgcgcc cttgctggtg cgccctgcca aaagaaagcg ctccccttcg
     6601 atggtgatcg ccggaccggg ggactcgaga gtgagcggca tgatgggcag ctgaaaggca
     6661 acctcgccgg acgccactcc gccaccaggc acattgatgc ggtagccaac cttgcggtcc
     6721 agccggcgcg ccatgttgtc gaggcccgat agctcgggag ttatatcgac ctcgtaggta
     6781 gcttgtccga gcgcgttcaa ggcgacggct ttgccgtcga ctgctacgct gctgccgggg
     6841 agtgcttcga cgaatacgct cagcgcaggc cgtgctttct ccaagccgcg cgtgtccgcc
     6901 tgcactcggt attcgagcgg aacgttgagt gccacgcgcg tctcgcggtc cgagcccggc
     6961 gcgcgcacga acagctcgaa acgattctct ccgacggata gcttccggcc caagcggacc
     7021 agcgctctcc cctgtttgat tgttcccagc tcttgtccga gccggacgcg cgttccgtct
     7081 ttgcacccgg cgcacgtcag gttcagcgac tctcggcctt cagcgtcgac tacggctcgg
     7141 gcctcgattc ggcccggccc gcgcaaccac aggaaagctc ccacgccggc tccggcgagc
     7201 gtgagcgctc caaacaatgc gaacagcccg agccaacgcc ctttacgggc cgacgcgacc
     7261 ggttgaaact cccgcgaatc ccggggcccg ccgggaaaga cctcattagg ggggctttcg
     7321 ttcgggaaga ccaggggcgg ggcgccaatt ggcgtgggta gaccaacggg gaataccgcg
     7381 ggctcctgct tggggacgcc ggggttcatc ggggcgatgc ccggccgcgc aaccccgagc
     7441 agtgttttca tcataggccc gcccggcggt gccgcggctt cggctacctg cggcgtttcg
     7501 ttctcgaacg aagcgggctg cgcaggcgtc actgcgctcg ggttggggtc cgggtttccg
     7561 gataaagccg caagtgggac ggtgccaccc cgcatgtctg gaggcgcaag acccagcacc
     7621 gtgcgtgcga agtgcggcgt ggcgttcgaa gggcgatcct cgaccgcctc gggccccgga
     7681 gttggggcaa gtccgatgat cgtcgctgcc atcggagggc gcgccgaggc gtccggcgca
     7741 acccgcacgg tgccaaccag ctgcggatcg agatgacctg cgacggtgcg tgcgagttct
     7801 tgcctgggct ccttcgccga tccggagttc ggttgccccc ccgccgaacg ggctccccct
     7861 gcgacggggg cctctatcac cgcgttctca gccgccgaaa ggggcaggcc gcaggttacg
     7921 caggagaagg ccgcgtcagt gttctccgtt ccacagcgcg cgcaagtctg catgccggca
     7981 gcactatacc ctgccgcccg cggggtgcgg tgagcctttg gcaaaaactc agtacctgct
     8041 ttccgcaagg tctttgtcca aaacccgtgc tttggcttaa agcccagaag cgaaagatgc
     8101 atgtgaccct ggctgacgta tggcgtgctg cccgagcgcg gcaggtgcca ttttctgcgg
     8161 aaagcgccgg gtacttggtg ctgtgtgcga tcgaggaact gagcgttttt ccacagctct
     8221 tcagcgcgaa tgaggtggag ctgtcggagg acggcagcgt gcgggtgtca cgtggcccag
     8281 cagcgacact tatcgaagcg gagcggtgcg cgcgtgcact gcttggaaac ctgctcgcgg
     8341 tttcttcttc gccagggccg gctttgtcgc gcgtggcagc ccgaagcgca gatgaaagcg
     8401 cccgttcggt gagcaggcat agaggcagcg aggggagcgc cggactcgcg gccttgggcg
     8461 cggaactcga aaatgccctc atccctctca accgcagcgc cgctcgtcgg gcgctgtgcc
     8521 ggttggcgcg cgagacaacc cgggctttgg gggccgggct gaatacggtg caacctgcac
     8581 cgcctgcctc gaaagcgagc tcatccgagt ctcgtgtttc ggcgccgctg cccgggccta
     8641 tccctgcgcc tgtgcagccc gttgcacccg atggcgccct cgacgctccg ctcaatccgg
     8701 gtgacagcgc agtcaacgag aaggggcgcg gtgtagagcg cgacagcgca cgccgtgggg
     8761 tccaaatcgc tctggttcga agaagtccaa cggagtcgtc tcctcagacg ccccgcttgg
     8821 gcacccggat cgacgctacc gcggatgagc tgagcgatga catcacccag cgcatgccga
     8881 atgtccaaac ggtgcccgcc gcccgggcgt cgtttacgga aacgcgtgtc ccgagtcggc
     8941 acctcgacac cagtgacaac gcccatccgc tcgaacgcgg tactcggccg cctggtgagc
     9001 aaactccgtt ttggggtgtg ccagccgtac acttgagccc gcgcgaggct ggcgcgaaca
     9061 aggctccgaa acccagtgcc accccgtccg taggcgcaaa gaacaaaccg cagttcacgc
     9121 ccgggcccga cgcgctcgcg gcgagctatg ccccaccgct gtcccctgaa gaagaggacg
     9181 agctcgagat tgagatcttg ccggagccgt tggagctctc tccctgccga gttccgccgg
     9241 tggagcccat gagtcacgag tgttctgcgg agccttcacc cgactcactc gacgttactc
     9301 ctgtggcgca ccacaagctg gtcgaggcgc tgcatgcgtg taccacctac gccggtcaag
     9361 gtgcccagcg cagcgacgtt agagagctga ttgccgactt tcaagtgcac gcggattgga
     9421 cggaggacga gctgagccgc gagttgaagg ggctggctca agtcgagctc acacccgcgg
     9481 gagccaatgc cggcccacgc gccaagtcgt cgcgccgttg acgccgagcg ggggtgtgcg
     9541 atcgtgcggg gccaatgcac gatcagaacc tcgcgatttt gaagggcttg gtgtgtgtgg
     9601 cttgggcgga cggggcgatg cacgcgaccg agcggcaact gctggaggcc ctcctgcagg
     9661 cgttcaacgc gaagcccagc gaagctttgg agatccgcct cttcgcggag cagccacgca
     9721 cgctgcagga cgtgcccatc cacgatctga gctacgccga tcggcgcatg ttgctcacgc
     9781 aagcggtgat tctgagcttt gcggacggcg aacaacacag caccgaacaa gtgatcatca
     9841 aggatcttgc gcgtgagttg cgtatcccgg atatcgaggc tcggggcatc attgcggcgg
     9901 ccgaggagca agctcgtcaa agcctcgaac tgctcgcgaa gtgaacgcga gctcgacgct
     9961 catccggggc gcggggctac tcgcaggcgc tcgatctcct tcagcagcgc gatggggctg
    10021 aagggcttgg tatagtaagc ggccgcgccg gcctgcgtgg cccgcgcgcg ggaatcatca
    10081 tcatccagcg cgctgatgat aacgacgggg atagcttggg tggttggatc agaacgcagc
    10141 cgcctacaca cctcgaaccc gtcgaatgga ccaggcagca ttagatccag taaaaccaag
    10201 tcaggtgggc tggttgcggc gacgtccaac gcttttaccc cggaactcac ggccatcact
    10261 tcatggccgc gcgcttggac gagcgcttcg atcatcttgc gaatcgaatc ctggtcctcg
    10321 caaacgagaa tcttcatcgg atctcgagaa tactctactc gacgtcgcgg cgcctcgaat
    10381 tgcgttcaat ctcgcagaac gcacatgctc ggtagctctg ccgctgtgtc gttttggaat
    10441 cgcggtcgtt ttggaatcgc ggtcgttttg gatcgcggtc gttttgaaac cgcgtcagct
    10501 ggcggatgca ccgtgcagcg taaaccagag ttcggttgcg cccaacgtcc cctcacgcag
    10561 ccgaagctcg gtgcctatcg cacgcaacgc cgcatgagca ccaggcaagg taatcccaag
    10621 cgggcgaccg acgctcgccg gatccgtatc gtggcgcacc acgctttccc agagctcgag
    10681 aggcacgggg ggaccgcgat cctc
//
LOCUS       DASTKU010001064        10325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1328314, whole genome shotgun sequence.
ACCESSION   DASTKU010001064 DASTKU010000000
VERSION     DASTKU010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328314"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFQ61_37845"
     CDS             <1..656
                     /locus_tag="VFQ61_37845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960334.1"
                     /translation="FSELPAIDMLGLNDRYIAHHPPPDLGKGWLAHEFGNGKYVLDRH
                     PDLVLFCGPAGRDKPCFRGGIEMQDDPSFASEYRLVTFEGATPYSFQSRIWVRLESAR
                     IGVRRSESQIVVPGFLFARGSGSIARLNDKQQLAAWIERNGTGRVERIELPTGTYCLR
                     AEASGDVTLSASNGVIDRSPSCLKLQLPAPTVLDIEVKPNIRDVWLDQLSVIRQKNP"
     gene            1053..2096
                     /locus_tag="VFQ61_37850"
     CDS             1053..2096
                     /locus_tag="VFQ61_37850"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET9960335.1"
                     /translation="MSATEARTPALAQAGESRALRVRVYVTRSYSAQVLDWQRRFVAT
                     LEDVTQLMRPVLRAPLELVATESWSPAGGEERVTDLLSELKIKDSGDDADWVIGLVGS
                     VPRFESSFHELGLAELLGKRIVLRAMNDSAEYQAILGGFPDLAEDERLKLYKKRLAHK
                     VTAVLLHEIGHTLGALHETGKQSLMAPVYAHTRTEFSPEALELMRFVLEHRSGIAGDP
                     EVRRAVVERWKREPSPFVSEARREAIASLTPAPASAVTAEALASKAAPGELAVPDRAS
                     FEQATVLLARGEITQAHAKASPLFARYPDSEAVQDLRCNIAMRLGGSWSQTSSECEPL
                     MRLMPNRKHSQGH"
     gene            complement(2125..3177)
                     /locus_tag="VFQ61_37855"
     CDS             complement(2125..3177)
                     /locus_tag="VFQ61_37855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960336.1"
                     /translation="MKAWLLLSGLGIACVSSRAQPSHEATVAGANPPAARVAPKSTEG
                     LVVFTGMCDASGAVPLSRDRFTVADDEDNVLRIYDAEVGGPPLSARDISRELGLTERK
                     RRKTGEPKPAPEVDIEAATRVGDLAFWLTSHGRDSSGRVRQERLRFFATTLPRGDSGP
                     IVVGKVRTDLLDPLFTDSRYATFELAAAARLPPKSAGGLNLEGLTERRDGGVWIGFRS
                     PTPEQKALLAPLLNPVEVTAGAAPNFGDPRLLDLGGLGVRGLSYWRGQYLILAGHHAG
                     GKTSKLYTWDGDHQPREERELGVDGFNPEGFFTPEDRDTFMILSDDGTRPIGGIECKR
                     LEESSQKSFRGLWLRL"
     gene            complement(3313..8952)
                     /locus_tag="VFQ61_37860"
     CDS             complement(3313..8952)
                     /locus_tag="VFQ61_37860"
                     /inference="COORDINATES: protein motif:HMM:NF023874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair ATPase"
                     /protein_id="HET9960337.1"
                     /translation="MADTNSAPPPAGGNYDVIRKRLLESARELRNRAEALNHRRQALF
                     GGRELALLSTERVRTENNCIPRDVVSLSGHLLFGFQVFIGLKSETRVEDVFSLHRFEK
                     TGDGYDLSSVPFEGPGSFLADAEFRKQFTDVFRYERDPRLLQLVRSDQRLLLVMQIGA
                     TLADRKVFRFSIDAQDRVKYVDARGEADHVPPRAHSFEWTLTTREDHVAGPFPHVNIL
                     DQVFVETIGGDLTLKVENNTQQGHGIYREPVDDPNQSLDDAEIAYARLGTLILLKVRP
                     FREQLYRYLIFNTRTQHVLRVDTIGKSCVELPENHGILFPNGYYLQSGEHKLFDGENS
                     DSEFERWVVAPNGEDVLYVFYRPEAGEYVLLPYNLIRKEINAPLRCHGYCLFPDGTML
                     VFREAPSREPSRIHPVQIWQTPFTTAEFAAAAPTDGSYLAKVGNRDLVSALSEVLALC
                     RAVEATPATRANYEDVIRGVQRLQDTHYWLGHEEVLGLLPALKQVGSAADLIVDEYEK
                     QREQERRAREAVETAAQQQRSLLEKTRPTDLTSVDAFLHALSDWKKQRGALITLKELT
                     GIDVTRIGELETEAVASFNAVSQACVEFFLGADAFAPLVARLDELVERAAQVTKAFEV
                     LPLREELDVVHEGLTLLAETIAGLSIQDSTQRTRILEGTSTAFSHQNRARALVEGRYR
                     DLSTSEKRADFAVQFKLLGQSVVTALGLANTPEACDEKLAALLLQLEELEGRFGDLDE
                     FAPAIAEKREEVLDSVATRRQALLEERKRRAGHVLAAAERMLMGIARRAAERASLDEL
                     NAYFATDSMVQKVRELGTELANLGEGVHAEEVEAKLKACRQNAQRTLRDRTDLFDPNA
                     NLIRFGQYQFQVNTQALELSLIARNGELHAHLTGTDFYEPVSHAALESYRELWDQELV
                     SESARVYRGEFLAVSLLRDAEQRVSGLSMDRLMQAHAEGTLLELVREWAAPRLDEGYE
                     RGVHDHDGCLVLQGLIGTWPNIGLLKYSPLARCSAWLHWDGLEPRARSALERAARSAG
                     HVKARFGDERPSRALAHDLIAAMLASLEVYPLAPWKGSLPFAAQYLIEELSQERPRFT
                     SSQAATELNRRFFEELERRGARRDFDEDLRAFSAERAAQLGTISNYMNAALGDAPNPF
                     ELALRLEAAARLMLDGKLEFQTSSASTRVELEGLLGAHPRIQARRLGYELPELLERVG
                     CFTIEIAPRYRTYRKLRAESADALRRELRLEEFTPRVLSSFVRNRLIDEVYLPLVGAN
                     LAKQLGAAGAGKRTDLMGLLLLVSPPGYGKTTLMEYVANRLGLIFVKINGPALGSAVH
                     SLDPAAAPNATSRQEVERINLALRMGNNVMLYLDDIQHTNPELLQKFISLCDGQRKIE
                     GVWRGQSRTYDLRGKRFCVVMAGNPYTETGARFQIPDMLANRADTYNLGDILAGQAEA
                     FAQSYLENALTSNPTLAPLAGRDPKDLHKLIELARGKEIPASDLSHAYSSSELQDIRQ
                     VLRHLFQIQDVLLRVNQEYIASASQDDNYRTEPPFKLQGSYRNMNKLAEKVVSAQTPD
                     EIERLIDDHYQSESQTLTQGAEQNLLKLAEIRGRLTPDQARRWTEIKETFVAVRRMGG
                     KTDDPVARLTGSLLGLDGQLKGIRETLASAVEGAAHQRERPSAGAVVGAAVGDAVARF
                     GAGIEPHLTSISEALGRVARPKLEVQIAGGWEPELVQLARQQLALLEQAVQALGARSS
                     QTGAPHAHAGSNGASSHELNAQLTQIHRTIEQLSNLVAQGGVGVREVEIALAAHSRSN
                     LIRPVTSLDVCGSGGGVFVATYDRPPPLGTPIRAHIQFPGNHQCQVDGRVRYHQDELG
                     EGSPAGYGVEFTRVEPEAAALLETFARLREPWIRDTD"
     gene            complement(8975..>10325)
                     /locus_tag="VFQ61_37865"
     CDS             complement(8975..>10325)
                     /locus_tag="VFQ61_37865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960338.1"
                     /translation="QNVKTNELRQKERMEIGSQNLSADEAVFRFDQRRAEAEAKKNKE
                     IAVAVAREENEALRIASEEQKKTLLIRIKNEEESSIADQAKQRGVAVAEKNREREIAV
                     ETERVEKARQLEVISREREVALLGISRDRDVEVQKKEIANVVRERISVEKSVAAEQEL
                     IKDVQALAEANRSKEKVRIAAEAQAQEGLVKQVKAAQAAEEVAKFKAKERMVTAEAEL
                     ETADKVARAKMRLAEGVQAEAAAEGMAKARVREADAAAFEKQGLVEARITLEKAQAHA
                     EGEQKQGLARVRVREAEATAIERQGQAEATAVRERLTAEAQGLHQKAEAMRALDESGR
                     GHEEFRLQLEKEQRIALEALNAKKEIAGAQAEVMARAFNNARINIVGGDGQFFQRFVN
                     AISLGQTVDSTLEQSDALRALVKEVTSHSGNGASLLSALDKLAPRPEPTDGKTTSRS"
BASE COUNT         1731 a         3294 c         3277 g         2023 t
ORIGIN      
        1 tcttctccga gctgcccgcg atcgatatgc tgggcctcaa cgatcgttac attgcgcacc
       61 atccaccgcc ggacttgggc aagggctggc tagcgcacga gttcgggaat ggcaaatacg
      121 tgctcgaccg tcatccggat ctagtgcttt tctgtggtcc ggcgggtaga gacaagccct
      181 gtttccgtgg cgggatcgag atgcaagacg acccctcctt tgccagcgag tataggctgg
      241 tcactttcga gggcgcgact ccgtactcgt ttcaatcacg catctgggtt cggctagaga
      301 gcgcgcgaat cggtgtgcga cgctccgagt cgcagatcgt ggtgccgggg ttcttgtttg
      361 cgcgagggtc gggcagcatt gctcgtttga atgacaaaca gcagctcgcg gcgtggatcg
      421 agcgcaacgg gaccgggcgc gtggagagga tcgagctacc cacgggaacg tactgcctac
      481 gggccgaggc gagtggagac gtgaccctat cggctagcaa cggggtaatc gaccgttccc
      541 cgagctgcct aaagctgcag ctgccggcgc ccactgtgct ggatatcgaa gtgaaaccca
      601 acatacggga cgtttggcta gatcagctaa gtgtaatccg gcagaagaac ccgtgaaccc
      661 taggcactct tcgcgagagt accaggatca tttcgcgcgt ttcgccggga taagtactat
      721 tggcctcgct gtgaagaacg tttatcgtgc ctcgctgagc gatggcacgc actgttggaa
      781 cgcgggagga gccgagaggc cgagccagga cgagagagcg cgctaacgaa cggcgcgagg
      841 gcgagcgcgt ggagagacgc ggggttgggc tcattgcgcg gttggcgggg ggtattgcgg
      901 tcctgtgttg cgcgagcggg tgcttctttt tcgattcgca atgggggcag caaaaggcct
      961 cccagcaacg ccttgcgcag cactacaccc ctcaaacttt gcggcgagcc gcgcccgaag
     1021 gcgctccggt gtctctggcg cagcccgcag ccgtgagcgc tacagaggcg cgaacgcccg
     1081 ccttggccca agccggcgag agtcgagctt tgcgtgtacg ggtttacgtc acgcgctctt
     1141 actcggcgca ggtgctcgat tggcagcgac ggttcgtggc gacgctcgaa gacgtgacgc
     1201 agctcatgcg cccggttttg agagcaccgc tcgaactcgt ggcgaccgag agttggtcac
     1261 ccgcaggggg cgaggagcgg gtgacggacc tgttgagcga actcaagatc aaggattcgg
     1321 gggacgacgc cgattgggtg attgggctcg tgggctcggt gccgcgcttc gagtcttcgt
     1381 tccacgagct gggcttggcc gagctgctcg gtaagcggat cgtacttcgc gcgatgaacg
     1441 actcggccga gtatcaggcc atccttggcg ggtttccgga tttggccgaa gacgagcgcc
     1501 tgaagttgta caagaagcgc cttgcccaca aggtcacggc ggttctgctg cacgagatcg
     1561 gacacaccct gggggccttg cacgagaccg gaaagcagag cctcatggct ccggtctatg
     1621 cgcatacccg caccgagttc tcgccggaag cgctcgaact catgcgcttc gtactcgagc
     1681 accggtcggg aattgccggg gaccccgagg tgcgccgcgc agtagtcgag cgctggaagc
     1741 gcgagccgtc gcccttcgtc tcggaggcgc ggcgtgaggc gatcgcctcg ttgactcccg
     1801 ctccggcgtc cgccgttacg gcagaagccc tggcatcgaa ggcagccccc ggggagctcg
     1861 ctgtccccga tcgcgcaagt ttcgagcagg ccacggttct cctggcgcgc ggggagatta
     1921 cccaggcgca cgccaaagcg agcccgctgt tcgcgcgtta tcccgactcg gaagcggtac
     1981 aagacctgcg ctgcaacatc gcgatgcgac tcggaggctc ctggtcgcaa accagctccg
     2041 agtgcgaacc attgatgcgc ctgatgccaa atcgcaagca cagccagggg cattgattgc
     2101 gttcatgcca gcagttttgc ggcgctaaag acgaagccaa aggcctcgaa agctcttttg
     2161 cgagctctct tccagcctct tgcactcgat tccgccaatg gggcgggtgc cgtcgtcact
     2221 caagatcatg aaagtatctc ggtcctctgg ggtgaagaac ccctctggat tgaacccgtc
     2281 cacgcccaac tctcgttctt ctcgcggttg gtgatccccg tcccaagtgt agagcttgga
     2341 ggtcttcccg ccggcgtggt gcccggccag aatcagatac tggccgcgcc aataggacaa
     2401 gccgcgaact cccagcccgc cgagatcgag taacctgggg tcgccaaagt tcggggcggc
     2461 accggcggtc acctccactg gattcaacag cggggcgagc agcgcctttt gctcaggagt
     2521 tgggctgcga aacccgatcc aaacgccgcc atcccttcgc tccgtcaaac cctccaggtt
     2581 gagccctccc gcgctcttcg gcggaagccg tgcggcagcg gcgagctcga acgtcgcata
     2641 tcggctatcg gtgaacagcg gatccagcag atcggttcga accttgccga cgacgatcgg
     2701 tcccgagtcg ccgcggggca aggtggttgc gaagaagcga agacgctcct gccgtacgcg
     2761 accgctgctg tcgcggccat gggaagtcag ccagaacgcc agatctccga cgcgagttgc
     2821 cgcttcgata tccacttctg gggccggctt cggttcgccc gtcttccttc tcttgcgctc
     2881 ggtcaggcca agctctcgag agatatccct cgcgctgagc ggcggcccgc cgacttcagc
     2941 gtcgtagatc cgcaacacgt tgtcctcgtc atccgcgacc gtgaacctat cgcgtgatag
     3001 gggtacggcg ccggaggcat cgcacatgcc cgtgaatacc accagacctt ccgtactctt
     3061 gggggcgact cgcgccgcgg gcgggttcgc gccggcgacc gtggcttcgt gcgacggctg
     3121 agcgcgcgag ctcacgcaag caatgcccaa tccgctgagg agcagccaag ccttcatcaa
     3181 ttgggctcta gcatggggcg aagcctgcct cggtgggggc ccaccgcgat cgggtgagtc
     3241 ttttcaccct ggactctgca gattgagccg ctcccagcgg ctagttgcgg ctcgaactca
     3301 cgcgcgacgg attcaatccg tatcgcgaat ccacggctcg cgcagccggg cgaaagtttc
     3361 gagcagcgcc gcagcctcgg gctccacccg ggtaaactcc acgccatacc cagcgggtga
     3421 gccttcgccc agctcgtcct ggtggtaacg aacccgccca tccacctggc actgatgatt
     3481 gcctggaaat tggatatggg cccgaatcgg tgtgccgagg ggcgggggac gatcgtaggt
     3541 cgcgacgaac acaccgcctc cgctgccaca cacatcgagt gaggtgacgg ggcgaatcag
     3601 gttcgagcgg ctgtgggcgg cgagcgcgat ttcgacttcg cgaaccccga ctccgccctg
     3661 cgccaccaag ttcgagagtt gctcgatggt gcggtggatt tgcgtgagct gcgcgttcag
     3721 ttcgtggctg ctcgcgccgt tcgaacccgc gtgcgcgtgc ggcgctcccg tctggctgga
     3781 gcgcgcgccg agtgcctgca ccgcttgctc caatagcgcg agctgctggc gcgcgagctg
     3841 aacgagctct ggttcccagc cccccgcgat ctgtacttcg agtttcggac gcgcgacgcg
     3901 ccccagggct tcggagatgc tggtcaggtg cggctcgatg cctgctccga atcgtgccac
     3961 cgcgtcgccc accgcggccc cgaccaccgc gcccgcgctc ggtcgttcac gctggtgcgc
     4021 ggcgccctcc acagcgctag cgagcgtctc acgaatgccc ttgagttgcc catcgagccc
     4081 gagcaagctg ccggtgagcc gcgcgacggg atcgtcggtc ttgccgccca tgcggcgaac
     4141 cgccacgaag gtctctttga tctcggtcca gcgtcgcgcc tgatcgggcg tcagacgacc
     4201 acggatttcc gcgagcttca gcagattctg ctcggctcct tgggtcaagg tttgcgactc
     4261 actctggtag tgatcgtcga tcaacctctc gatttcgtcg ggggtctgcg ccgagaccac
     4321 cttttcggcc agcttgttca tgtttcgata gctaccttgt agcttgaacg gcggctccgt
     4381 ccggtagtta tcgtcttgcg aagcggaagc gatgtattcc tggttcactc gcaagagcac
     4441 gtcttgaatt tggaacaagt ggcgcagtac ctggcggatg tcctgcaact cgctgctcga
     4501 gtaggcatgg gacaagtcgg aggcggggat ttccttgccg cgcgcgagct cgatcagctt
     4561 gtgtaagtcc ttggggtccc gaccggcgag cggcgccaga gtggggttcg aggtcagcgc
     4621 gttctccaaa tagctttgcg cgaacgcctc ggcctgcccg gccaagatat ctcccaggtt
     4681 ataagtatcg gcgcgattcg cgagcatgtc gggaatttga aagcgggcac cggtctcggt
     4741 gtacgggttg cccgccatca ctacgcaaaa gcgctttccc cgtagatcat aggtgcgaga
     4801 ttgacctctc cacacaccct cgattttgcg ctggccgtca cacagcgaga tgaacttctg
     4861 gagcagctct ggattcgtgt gctggatatc gtcgagatac agcatcacgt tgttgcccat
     4921 cctgagcgcc aggttgatgc gctctacttc ctgccgagag gtcgcgttcg gcgcggcggc
     4981 gggatctaga gagtgcacgg ccgaccccag ggcagggcca ttgatcttga caaagatcag
     5041 gccgagccgg ttggccacgt actccatgag agtggtcttg ccgtatccag gaggggaaac
     5101 gagcaacagc aaacccatca agtccgtgcg cttccccgca ccggcggccc ccagttgctt
     5161 ggcgaggttc gctccgacga gcggcaagta cacctcgtca atcagccggt tcctgacgaa
     5221 ggaggagagc acacgcggcg tgaattcctc gagcctcaat tcgcgacgga gcgcgtccgc
     5281 ggattccgca cgcaacttgc gataggtgcg gtagcgagga gcaatttcga tcgtgaagca
     5341 gccgactcgc tcgagcagtt ccggcagctc atagccgagg cgccgggctt ggatgcgcgg
     5401 gtgcgcaccc aacagaccct cgagctcgac gcgcgtcgat gctgacgagg tctggaactc
     5461 caacttgccg tcgagcatca ggcgcgccgc ggcttcgagc cgcaaggcca gctcgaatgg
     5521 attcggcgcg tcccccaaag cggcgttcat atagttcgat atcgtgccaa gctgcgcggc
     5581 gcgctcagcg ctgaacgcgc gcagatcctc gtcgaaatcg cgacgtgcgc cgcgtcgctc
     5641 caactcctcg aaaaacctcc ggttcagctc ggtggcggcc tgactggacg taaagcgcgg
     5701 acgctcttgg ctgagctcct cgatcaggta ctgggccgcg aatggcagcg agcccttcca
     5761 gggcgcgagc gggtaaacct ccaagctcgc cagcatggcc gcgatcaagt cgtgggccag
     5821 cgcgcggctt ggccgttcgt caccgaagcg tgctttcacg tgtccggcgc tgcgcgcggc
     5881 gcgttcgagt gcgctgcgtg ctcgcggctc cagcccgtcc caatgtagcc acgccgagca
     5941 gcgcgccaac ggtgaatact tcaacagccc aatattcggc caggtgccga ttagaccctg
     6001 cagcaccaag cagccatcgt ggtcatgcac gccgcgctcg tagccttcgt ccaagcgcgg
     6061 cgcggcccac tctcggacaa gttccaggag cgtgccctcg gcgtgggcct gcatgagccg
     6121 atccatgctc agacccgaca ctcgctgctc cgcgtcgcgc agcagcgaca cggccaggaa
     6181 ctctccgcga tagactcgcg cgctctcgga tacgagctct tgatcccaca gctcgcggta
     6241 actttcgagg gcggcgtggc tgacgggctc gtagaaatcc gtgccggtca aatgggcatg
     6301 cagctcgccg ttgcgggcga tcaggctcag ctcgagcgct tgagtgttga cctgaaattg
     6361 atactgcccg aatcgaatca ggttcgcgtt tgggtcgaag agatcggttc gatcgcgcaa
     6421 cgtacgctgc gcgttttggc ggcacgcttt gagtttggct tcgacctctt ccgcgtggac
     6481 gccttcgccc agattcgcga gctccgttcc gagctcgcgg accttctgta ccatcgagtc
     6541 cgtggcgaag taggcgttga gctcgtcgag gctcgcgcgc tccgccgcgc ggcgcgcaat
     6601 gcccatcaac atccgctcgg ccgcggccag cacgtgaccg gcgcggcgtt tgcgctcctc
     6661 gagcagggct tggcggcgcg tggcaaccga atcgagcacc tcttcgcgct tctcggcgat
     6721 agcgggtgcg aactcgtcga ggtctccgaa ccgaccctct agctcctcca gctggagcag
     6781 tagggctgcg agcttctcat cgcaggcttc gggggtattc gccaagccga gcgccgtgac
     6841 gacgctttgg ccgagcagct tgaactgcac cgcgaaatca gcgcgcttct cggaggtcga
     6901 gagatcgcgg tagcgcccct ccaccaacgc gcgggcgcgg ttttggtgtg agaatgcagt
     6961 gctagtccct tcgagaatgc gagtacgctg ggtgctgtct tggatcgaga ggcccgcgat
     7021 cgtttccgcg agcaacgtga gcccctcgtg gaccacatcg agttcctcgc gcagcgggag
     7081 aacttcgaac gccttcgtca cctgtgccgc tcgctcgacc agctcgtcta gccgggcgac
     7141 gagcggcgcg aaggcgtccg ctccgagaaa gaactcgacg caggcctggc tcacggcgtt
     7201 gaagctcgcg acagcttcgg tttcgagctc cccaatgcgg gtcacgtcga ttcccgtgag
     7261 ctctttcagg gtgatgagag ccccgcgttg cttcttccaa tcactgagtg cgtgtaggaa
     7321 ggcgtcgacg gacgttagat ccgttgggcg agtcttctcg agtaggcttc gctgctgctg
     7381 agccgcggtc tcgacggctt ctcgcgcgcg gcgttcctgc tcgcgttgct tttcgtactc
     7441 gtcgacgatc agatccgcgg cgctgccgac ttgtttgagc gccggtaaga ggccgagcac
     7501 ctcctcgtgc ccgagccaat aatgggtgtc ctgtagtcgc tgaacgccgc ggatgacgtc
     7561 ctcgtagttg gcgcgagtcg ccggagtcgc ttctacggcg cggcacagcg ccagtacctc
     7621 gctcagcgcg gacaccaggt cgcggttccc taccttcgcc agatacgaac catccgtcgg
     7681 ggcggcggcg gcgaactctg ccgtggtgaa cggagtctgc cagatctgta ccggatgaat
     7741 ccgactgggc tcgcgcgaag gggcctcgcg aaataccagc atcgtgccgt ccggaaagag
     7801 gcagtatccg tggcaacgca ggggtgcgtt gatctccttg cggatcaagt tgtaaggaag
     7861 cagcacgtac tcaccggcct cggggcggta aaatacgtac aatacatctt caccgttcgg
     7921 agccaccacc caacgctcga actcggagtc cgagttttca ccatcgaata gcttgtgctc
     7981 gcccgactgg aggtaatacc cattcgggaa cagaatgccg tgattctcgg gcagctcgac
     8041 gcaggatttg ccgatggtgt ccacccgcag tacgtgttgg gtgcgagtgt tgaagatgag
     8101 atatcgatac agctgctccc ggaagggacg caccttgagc agaatcagcg tgccaagccg
     8161 ggcatacgcg atctcggcgt catcgagcga ctgattcggg tcgtctaccg gctcgcggta
     8221 gatgccgtgg ccctgttggg tgttgttctc gaccttcaga gtcaggtccc cgccgatggt
     8281 ttcgacgaag acttggtcca agatgttgac gtgggggaac ggacccgcga cgtgatcctc
     8341 gcgcgtggtg agcgtccact cgaaagagtg agcgcgcggc ggtacgtgat cggcctcgcc
     8401 gcgcgcgtct acgtacttca ctctatcctg cgcgtcgatc gagaagcgga agaccttacg
     8461 atccgcgagc gtcgcgccga tctgcatgac gagcaatagg cgctggtccg aacgaacgag
     8521 ctgcagcaga cgcggatctc gctcgtagcg gaagacatcc gtgaattgct tgcggaactc
     8581 cgcgtcggcc agaaagctgc ccgggccttc gaacggcacg ctgctcagat cgtagccatc
     8641 cccagtcttc tcgaagcggt gcagactgaa gacatcttcg acccgagttt cgctcttgag
     8701 cccgatgaag acctgaaacc cgaacagcag atgccccgac aggctcacga cgtcccgcgg
     8761 aatgcagttg ttctcggtgc gcacgcgctc ggtcgagagc agcgccagct ctcgaccgcc
     8821 gaacagcgcc tgccggcgat ggttcagggc ctctgcgcgg tttcggagct ctcgcgcact
     8881 ttcgagcagg cgcttgcgga tgacgtcgta gttgccgccc gccggtggcg gcgcgctatt
     8941 ggtatcggcc atgagagatc aacccgcgcg gggctcagct gcgagaggtg gtcttgccgt
     9001 cggtgggctc ggggcgtggg gcgagcttat cgagtgccga gagcagagaa gcgccgttgc
     9061 cagagtggct cgtcacttct ttcaccaggg cgcgcaaggc gtcgctctgt tcgagcgtcg
     9121 agtccacggt ttggccgagt gagatggcgt tcacgaagcg ctggaagaac tgcccgtcgc
     9181 cgccgacgat gttgatacgc gcgttgttga aggcgcgggc catgacctcg gcctgagccc
     9241 cggcgatttc cttcttggcg ttgagcgctt cgagcgcgat gcgctgctcc ttttcgagct
     9301 gcaggcggaa ctcctcgtgt ccacgcccag actcgtcgag tgcgcgcatc gcctcggcct
     9361 tctggtgcag gccctgcgct tctgcggtca agcgttcgcg cacggccgta gcttccgcct
     9421 ggccttggcg ctcgatcgcg gtcgcttcgg cttcccgcac ccgcacccgc gcgagccctt
     9481 gcttctgttc gccttcggcg tgggcttgcg ccttctcgag cgtgattcgg gcctccacca
     9541 agccctgctt ctcgaacgcg gcggcgtctg cctcccgcac ccgcgccttg gccatgccct
     9601 cggcggcggc ctcggcctgc acaccttcgg ccaaacgcat cttggcgcgc gccaccttgt
     9661 ccgcggtctc gagctctgcc tcggccgtga ccatgcgctc cttggctttg aacttcgcga
     9721 cctcttccgc ggcttgggcg gccttgacct gcttcaccaa gccctcctgg gcttgcgcct
     9781 cggccgcgat gcggaccttt tctttcgagc gattcgcctc ggccaaggcc tggacgtcct
     9841 tgatcagctc ttgctcggcc gcgacgctct tttctacgga gatccgctcg cgaacgacgt
     9901 tggcgatctc cttcttttga acctcgacgt ctcggtccct cgagatgccg agcagcgcca
     9961 cctcgcgttc gcgactgatg acctcgagct ggcgcgcctt ctcgacgcgt tcggtctcga
    10021 cggcgatctc gcgttcgcgg ttcttctcgg ccacggccac gccgcgctgt ttggcctgat
    10081 ccgcgatcga gctctcttct tcgttcttga tccggattag caacgtcttc ttctgctctt
    10141 cgctcgcgat gcgcaaggcc tcgttctcct cgcgcgccac ggcgacggcg atctccttgt
    10201 ttttcttggc ttcggcctcg gcccgccgct gatcgaagcg gaacacggcc tcgtcggcgc
    10261 tcaaattctg gctgccgatt tccatgcgct ccttctggcg cagttcattg gtcttcacgt
    10321 tttgg
//
LOCUS       DASTKU010001065         4797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_433160, whole genome shotgun sequence.
ACCESSION   DASTKU010001065 DASTKU010000000
VERSION     DASTKU010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433160"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="VFQ61_37870"
     CDS             complement(<1..1377)
                     /locus_tag="VFQ61_37870"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET9960339.1"
                     /translation="MNAVDRNLRSAAFVYLKPKATRGLRPSPAGIGQGEALFSSVMRA
                     FGLLIGSMFSVLGAASLTTTGCGDTGAESRPRTTGSGGAIANGGQTVVPQGGQTITGA
                     GGMTFLPPPSSNSGGMTGPETGCNDRNIVKTPVTPTVLLLVDNSSSMFEPREQLWDLL
                     YSTLMDPAMGIVKGLEDKIRFGFASYKGNRVATMETDPKCAEITSVPYALNNFQAIDT
                     TYKKLGTEWMQGVKWETPTGFAINQVAAQLQAFTADPPGPKYILLVTDGNPNTCQTVD
                     PQCGQDLSIQAVQNAYAKGIGTFAIGIGTIVANDPGCDKSWGRCGIEHLQDIANAGVG
                     LPVAPPPENYRYQTCSQPFGNMLQATYAPSATGLTNAPYFTATNAAQLKTALSGLLNG
                     VVSCSFDMDHEVTGDPSLGTVMLSGAPLTYTSEPAATSGWRLDGSHQVTLLGTSCETF
                     KATTGELNI"
     gene            complement(1495..3423)
                     /locus_tag="VFQ61_37875"
     CDS             complement(1495..3423)
                     /locus_tag="VFQ61_37875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960340.1"
                     /translation="MRSLPFLITGLFVTAPALAQEEADTAETKDASAETKEAEKAEAP
                     KDAPAAEAKAAATVQLGSTNAAKSEAEGAAVAAPAAKTSPSQRGATSASDDSWKFEFH
                     GYLRAPMRVGMGHRDTTLPGQSASTLHYPVIPDDQYLSWQFTSHNKKDWAEIFFSYGN
                     SWAKGTVALQGFNFTDAAWTDSPAQFGISQGFVTLTPDLGFENVRLEAKAGSFWGRYG
                     MAGRWDSGEYDTYVMGRTHVMGEALHLEIDLDDANTLWLEHGIGTKRPDPNFNNNARF
                     TLLNHAHAGFRLGSDIEISAHYMHAWAQEEDRIMRKTNTYDGTALLDPYNAPTYPTSV
                     HGVPDGKLWVAGIDARMELGPVGFVYLGYSHIGARDAIVVAPALEVLHGYGGGQFTLG
                     VTDNYFGPSCKASNNSPYENKELVGGYPGPLASECSRGTGSVNSIVGQWDFSLTNFTQ
                     QIGGGQRFWGDGQDLRAVLYGMVNFVNSDSHLKGKNGVEIFQDNMKLKYGIDLQYQAL
                     PWLTIAARADRVQPNKNVPEQSFSVVSPRLIFKSNWNTHEQISISYSRYFYNQRDCEA
                     GANPAYVVTKPEMSLPGSYGAHQYTPNQFYCIQPPSSSPSDEGYGSHYEKQSAGLRGA
                     PTTRPDVNVIKVEASMWW"
     gene            complement(4160..>4797)
                     /locus_tag="VFQ61_37880"
     CDS             complement(4160..>4797)
                     /locus_tag="VFQ61_37880"
                     /inference="COORDINATES: protein motif:HMM:NF027675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HET9960341.1"
                     /translation="SVGETASPAPRLEVEAGTSNLPRKRRRAQLRPWLRALHRDIGYL
                     AVGFTFIYALSGLAVNHIADWDPNFHESVRTHQLGSPLKGEDGPVSARVLKELEINET
                     PRELYRASDSRLEIVFDKRALHVDTATGVVVEEAQKPRFFLRVANYLHLNRGKKAWTY
                     VADAYAVFLLFLAISGLFMIPGRKGLFGRGAVLTALGIAVPALYVVLSRAP"
BASE COUNT          862 a         1455 c         1517 g          963 t
ORIGIN      
        1 gatgttcagc tcgccggtag tggccttgaa ggtctcgcac gaggtgccga gcagcgtcac
       61 ctggtgcgag ccgtccagac gccagccgct cgtggccgct ggctctgacg tgtaggtcag
      121 tggcgcgccc gacagcatca ccgtgcccag tgaaggatct ccggtgacct cgtggtccat
      181 gtcgaagctg caagacacta ccccgttgag cagccctgac aacgccgtct tcaattgcgc
      241 ggcgttggtg gcggtgaagt acggcgcgtt cgtcaggccc gtagcgctcg gtgcgtaggt
      301 cgcttgcaac atgttgccga acggttgcga gcaagtctgg tagcggtagt tctccggcgg
      361 gggagcaacg ggcaggccta cacccgcgtt ggcgatgtct tgcaggtgtt caatgccaca
      421 acgcccccaa ctcttgtcgc agccgggatc gttggccacg atggtgccga tgccgatcgc
      481 gaaggtcccg atgccttttg cgtaagcgtt ctgcacggcc tgaatgctca aatcctgccc
      541 gcactgcgga tccacggttt ggcaggtgtt ggggttccca tcggtgacga gcaggatgta
      601 cttgggtccg ggcggatcgg cggtgaacgc ctggagctgc gcggcgacct ggttgatggc
      661 gaagcccgtg ggcgtttccc acttcacgcc ctgcatccac tcggtgccca gcttcttgta
      721 cgtggtgtcg attgcctgaa agttgttcag cgcatacggc acggacgtga tctcggcgca
      781 cttcggatcc gtctccatcg tggcgacgcg gttgcccttg tagcttgcga acccgaaacg
      841 gatcttgtcc tcgaggcctt tcacgatgcc catcgcggga tccatcaacg tgctgtagag
      901 cagatcccaa agctgctcgc gtggttcgaa catgctcgac gagttatcga ccaacagcag
      961 caccgtcgga gtgacggggg tcttgacgat gttgcggtca ttgcagcccg tctccggccc
     1021 agtcatgccg ccgctgttgc tggacggcgg aggcagaaac gtcattccac cggccccagt
     1081 gatcgtttgc ccgccttggg gcaccaccgt ctggcctccg ttggcaatgg caccgccaga
     1141 tccggtcgta cgcgggcgtg actccgcacc ggtgtcaccg cagcccgtag tagtcaacga
     1201 cgcagcaccc aggacgctaa acatcgagcc aataagcaag ccgaaagcgc gcattactga
     1261 cgagaatagc gcctcaccct gaccaatgcc agctgggctc gggcgcaacc cgcgagtggc
     1321 tttgggcttc agatacacga acgccgcgga gcgcaggttg cggtccacgg cgttcattca
     1381 agctcaatca agctatgcgc acgagccgcg cgagggaaag atgattgcct cgcgcgactg
     1441 aagtccaggt gagatcaagt gaaggcgccc gcttccaagc agacgctctt cgcgtcacca
     1501 ccacatgctg gcttcgacct tgatcacgtt gacgtcggga cgcgtggtgg gggcaccgcg
     1561 cagaccggct gattgcttct cgtagtgcga cccataacct tcgtccgacg gcgaagagct
     1621 gggcggctga atgcagtaga actggttggg cgtgtactgg tgggcgccgt aggaaccggg
     1681 cagggacatc tcgggcttgg tcactacgta agctgggttc gcgcccgctt cgcagtcacg
     1741 ttggttgtag aagtagcggg agtacgagat gctgatttgc tcgtgggtgt tccagttcga
     1801 tttgaagatc aggcgcggag acaccaccga gaaggactgc tcgggcacgt tcttgttcgg
     1861 ctgaacgcgg tcggcgcggg ccgcgatcgt gagccaaggc agcgcttggt actgcaggtc
     1921 gatgccgtac ttcagcttca tgttgtcttg gaagatctcg acgccgttct tccctttgag
     1981 gtgcgagtcc gagttgacga agttgaccat gccgtagagc acggcgcgta ggtcttggcc
     2041 gtcaccccag aagcgctgcc cgccgccaat ttgctgcgtg aagttggtga ggctgaaatc
     2101 ccactgccca acgatcgagt tgacggagcc ggtgccgcgg ctgcattcac ttgcgagggg
     2161 gccggggtag ccgcctacta gttctttgtt ctcgtacggc gaattgttgg acgccttgca
     2221 gctcggacca aagtagttgt cggtcacgcc gagcgtgaac tggccgccgc cgtagccgtg
     2281 cagtacctcg agcgccgggg ccacgacgat cgcgtcgcgc gcaccgatgt gcgagtaacc
     2341 gaggtacacg aagcctacgg gcccgagctc catgcgagca tcgatacccg caacccagag
     2401 cttcccgtcc ggcactccgt gaacggaggt cgggtaagtc ggcgcattgt acggatccaa
     2461 caacgccgta ccatcgtacg tgttggtctt ccgcatgatg cggtcttctt cttgagccca
     2521 agcgtgcatg tagtgcgcgc tgatctcgat gtccgagccc aagcggaagc ccgcgtgggc
     2581 gtggttcagt agcgtgaagc gcgcattgtt gttgaagttc ggatcgggac gcttggtgcc
     2641 gatgccgtgc tccagccaca acgtgttcgc gtcatccaaa tcgatctcga ggtgcagcgc
     2701 ctcacccatg acgtgggttc gacccatcac gtacgtgtcg tattcgcccg agtcccagcg
     2761 cccggccatg ccgtagcggc cccagaacga gccggccttc gcctctagcc gcacgttctc
     2821 gaagccgaga tcgggggtca aagtgacgaa accctgagag atgccgaact gcgctggcga
     2881 gtccgtccag gccgcgtccg tgaagttgaa gccttgcagc gcaacggtgc ccttcgccca
     2941 gctatttccg tagctgaaga agatttccgc ccagtctttc ttgttgtgcg aagtgaattg
     3001 ccagctcaag tactggtcgt ccgggatgac cggatagtgg agcgtgctcg cgctctggcc
     3061 aggcagagtg gtgtcgcgat gccccatgcc gacgcgcatc ggcgcgcgga gatagccgtg
     3121 gaactcaaac ttccagctgt catccgaagc cgaggtcgcg ccgcgttgcg agggggaggt
     3181 cttggcggcc ggcgctgcga ccgccgcgcc ttcggcttcg gacttcgccg cattcgtgga
     3241 gccgagctgc acggttgcag cggccttggc ctctgctgcc ggcgcgtcct ttggggcttc
     3301 tgctttctct gcctctttgg tctcggccga agcgtccttc gtttcagccg tatccgcttc
     3361 ctcttgagct agagccgggg cggtgacgaa caagcctgta ataaggaatg ggagactgcg
     3421 catcgtagat cgctcgctag ttgaatcgct cgccggggtt tcgctgagct ctgggtctgg
     3481 gaccggtcat tcgaatcgag ccagattggt cgatccgggt gggggaagcg gtcttcgccg
     3541 agctcgggcg tgagaccgat cggatccgtg gttccgcgga taacagtgtc cgcaagtaac
     3601 gaagtctgca gagatcgagt tctgcagagc cgatccggtg aggccgatcc tgctgttaac
     3661 tagtcatgtc gtcgaccagt tgcgccgatc ggctcgattg caggttgctg tactttggcg
     3721 agcgcgtctt catagcaggg tctcccggaa tcgggaagtg ccgagagggc atcggacatg
     3781 tcgaaaatgc gagccccgtc gagaccccga acctcctctc ggattcgctc gactttcgcc
     3841 tcaaattcgc gcgcaatacc cgctgttctc gtgctcgctc gggattggag cgaatcgact
     3901 cgagccagca ctactcagca cgatcacgga gcctctcgag aggaaccttc tcgaactgac
     3961 ttcggtcctt cgcaggcgcg ttctcagcgc gagtcggctg ccccccggta gcgcggcaga
     4021 tggttcgggg gagaccgcgc tgcagatcga aaaaggatga tcgtttggcg cacacccgca
     4081 catctccccg atttgccctc gattgcgggc tcgaaccttg aggctgacgc gatgcgcagg
     4141 ttgccccgcg tactccggct tagggtgcgc gagagaggac cacgtaaaga gccggcacgg
     4201 cgatgcccaa cgccgtcagc acggcaccgc gcccgaacaa gcctttacgc cccgggatca
     4261 tgaacaaccc cgaaatcgcc aagaacaaca agaaaaccgc gtacgcgtct gccacgtagg
     4321 tccaggcttt ctttccgcga ttcaggtgca agtagttcgc cacccgcaag aagaagcgcg
     4381 gtttttgcgc ttcttcgacc accacgccgg tcgcagtgtc tacgtgaagc gcgcgcttgt
     4441 cgaacacgat ctcgagccga gaatcactcg cgcggtagag ttcacgcggc gtctcgttga
     4501 tctcgagttc cttcagcacg cgagccgaca cggggccatc ctcacccttg aggggtgagc
     4561 ccagctggtg tgtgcggacg ctctcgtgga agtttggatc ccaatccgcg atgtgattta
     4621 cggccaaacc cgagagcgca tagatgaaag tgaacccgac ggcgagatag ccgatgtcgc
     4681 ggtgcaaagc gcgcagccac ggacgcaact gcgctcgcct acgtttgcgc ggcaggttgg
     4741 aggtgcctgc ttccacctcc aatctaggcg ccggcgaagc agtttctccc acgctcg
//
LOCUS       DASTKU010001066         8607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1104794, whole genome shotgun sequence.
ACCESSION   DASTKU010001066 DASTKU010000000
VERSION     DASTKU010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8607
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104794"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1119)
                     /locus_tag="VFQ61_37885"
     CDS             complement(28..1119)
                     /locus_tag="VFQ61_37885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET9960342.1"
                     /translation="MADSWVASASAGATSVRANDLLPLLDVLSDPLLRVSSSGLLLAF
                     RPSRSRCDPADILPDLSEAVGQKLEEWLPPEVATLWRKHLAEALTSGQPQEFDFEWHA
                     PRGAFWLTARMNASAHADEALVVLRDRSRERRLNDENAQLKAALEEKDTLLREVHHRI
                     KNHLQVISSLLNIQLNRTDQRQARVVLEEAGNRVRSISILHESLYQHPLPSRVDMREY
                     VSKLVRNLTRTYVGVSHRVLVRDQIAPLALPVDLALPCGLIVNELLTNAFKYAFPSDR
                     RAGGEVRLSLTGSGPIELRIEDDGVGVSDVEAWRHASSVGMSLVRALSRQLDARLELK
                     SDASGTSVRTIFDPDRASALRSVPPLSSP"
     gene            complement(1244..2113)
                     /locus_tag="VFQ61_37890"
     CDS             complement(1244..2113)
                     /locus_tag="VFQ61_37890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960343.1"
                     /translation="MVIEIDAAAEHWVDPRATRRLVALEVSDVDVPPARAGQMPTLFY
                     RVLAEPNGGVRVELWERGEFHDVRRVTGDLGNLQIAARRVALAAAELARGLRQKRAQQ
                     QRARADRQRLWAELAQVRANRTVEGPFALRSSLFVTQSREWTSFGSTLAFETNIHHKL
                     RLDWAFSASGGQAHSGPWVSVLDLTLGPARRFSLTPRVDLSVAAWLGVGLLHVSRVSS
                     LDDMARQHESWTARVGATLRAEPRLSRRLRANLGVSGGAYLRRVPSSDPAIERIFDGG
                     FLGLELGLITTPP"
     gene            complement(2178..3251)
                     /locus_tag="VFQ61_37895"
     CDS             complement(2178..3251)
                     /locus_tag="VFQ61_37895"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9960344.1"
                     /translation="MIDDPLSELSKTALRDYAGDPRLDRVWQRLSSEAGREKRRSRAP
                     LFFVPALGAVTFAVGIWVGRQTVVTPVPEPAFLAEQPVAMSPAGGVRMPVEAPKSTPA
                     VPPSAGVRRPVAGRSPSHSAYNRPLEPAMAGDPGYAGPASSSAGAGAQQKNWQQKAWQ
                     LKADAGDFAGARTELERAGGWDAALFSASPDQLMTLVDVARASGERDQAIRALRRLLS
                     SFPSVPEAPLAAWTLGNLLEQAGDRAGAAEAFAQYRRLSPAGDFAEDAAARQIDAALS
                     EGKLVFAAELVDQYEKDFPHARRLHELRLELRKLQGEQAAPNGSPGGAAPGANNGSSS
                     ESRGEAAADSQAEPRSPSAEQSP"
     gene            complement(3248..3943)
                     /locus_tag="VFQ61_37900"
     CDS             complement(3248..3943)
                     /locus_tag="VFQ61_37900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9960345.1"
                     /translation="MARVRDMPKLRLVGGRDGQPEERPQRARREQPSLFDDLTDAQVV
                     ALVMDGDRDAFEALYRRHAAYALGLAVRIQGGAGDVEDIVHDAFIRAHDSLANLKDAS
                     AFRAWLGSIVVRLVRTRLRRRRLLGSLGLIAPEPVDLDAVAAPDADTESRVLLAQIYA
                     LLQTLPASDRIAWTLRFIERHRLESVAELMDCSLATAKRRIQRAQRFLSGHFVAPFSK
                     EPLSKEMLSKDDR"
     gene            4219..4458
                     /locus_tag="VFQ61_37905"
     CDS             4219..4458
                     /locus_tag="VFQ61_37905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960346.1"
                     /translation="MSIRKFAALIPVAVLMAACGGEATPEAKAPEGGAAAAEEAKPAE
                     GDAAAAAGDKAAEGADKAAEGADKAADEAKPAESK"
     gene            complement(4651..6072)
                     /locus_tag="VFQ61_37910"
     CDS             complement(4651..6072)
                     /locus_tag="VFQ61_37910"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9960347.1"
                     /translation="MPEPAPSEPDRRASAARVAKWLVERERPDEAVALLSAWAATGPN
                     DKAGQELLAEALRIDPSAPVAQQAFERMQGVAGDHSELDTCVVRFGSDELARLEAELR
                     RPSFRRAQMGFNNNIKFQGEVFHVQTEDSGLDQPHIVTHLFADGGRVVKSHKRSYATE
                     VSRSDVAEYVRQLMKAQHLEMVMSLREGRFDEILAGRAVGGLEVLREPPNIDNIRKLA
                     SQKKQRAESAHAAPSDAPRSEVVPSERGERPERAARSERGARSRFTLHVLRSLTGGPD
                     RYEPRGLATLIGAKGEVTLPGERFCHPEEARLEFRNERLWLDDLGGGNGVFLRIRTPV
                     ELNLGDEFVVGDQLLRVERNPVPDDGPDEHPTYFYSSPKWPSSFRVQQILEGGPLGCC
                     VVARGNTLQIGSAIGDLVFPDDPLVSEQHCLIEEHAGTVVLTDLGSRTGVFVRIRGEQ
                     ELTRGDELLVGRTRLVVELPEVA"
     gene            complement(6220..7800)
                     /locus_tag="VFQ61_37915"
     CDS             complement(6220..7800)
                     /locus_tag="VFQ61_37915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960348.1"
                     /translation="MTYGLNLARCSRLAVFASWGLVLMLGCGRAELPLEGPDDLPAGT
                     GGTGGSGGAIKGGTSGTARGGAGGTTFKTSGGASGAGAAPNGGGKTAGGSTFGGSPFG
                     GGGFGGTGGWVGGASGAGGSSGATWCGNGLCEPGESLKSCADDCHFCGDGICSPSEKK
                     DCASDCPQCGDMICQPSEVGRCIECKQLGCGNGICEPNDATWCGDCPISACGDGYCSQ
                     AEVGQCTRDCPQCGDGVCSPMESGWCWDCSNACNFDGVCQSFESAASCKDCFCGNGTC
                     DAEESVFTCAFDCSVCGNLVCEGNENPSNCSRDCASCGDGICSDLFENLDTCPSDCSV
                     CGDGICSWLETADSCSADCGTCGDSICQGVESVDWCPTDCTACGDGLCTFDETRASCR
                     LDCSCGNGTCDLDEDARTCPNDCNRCGDGVCTSFDGPGCTVDCPVCGDELCTGVEADG
                     SCPFDCAGCGDGKCTGLENQTTCPRDCPGLEICGDRVCNGFESLTCAFDCGVLETCGN
                     ERCEPSDLGVCNEDCGIF"
     gene            complement(7881..>8607)
                     /locus_tag="VFQ61_37920"
     CDS             complement(7881..>8607)
                     /locus_tag="VFQ61_37920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960349.1"
                     /translation="VRVVVRVKGTDGAGASSEAEHPFQVQPANLASVTPRGSLTCQRS
                     GDGAVSLTWGANTRQVALRCRLVDRVDASDVGRVELTAGAFKPKVRVLAKGGAELDGV
                     DVVLTSRNTGVLVARGAELVPKSVGSAVILARAGQAAREFKVDVVRKLTPEALPIDGN
                     RKIHFSLEPGSYELKVALPTAKSVSVEWRGAPYCNYSGVAQEHVSKCVLRAKGGAVFD
                     NPAYLLARSTEVSTEGVSLHEVP"
BASE COUNT         1607 a         3031 c         2539 g         1430 t
ORIGIN      
        1 cactacggct ccactcactt caccgcgtta cggcgaagag agcggcggca cgctacgcag
       61 cgcgctggcc cggtccggat cgaagatcgt gcgaacgctg gtgccgctgg cgtcgctctt
      121 cagctccaat cgcgcgtcga gctgccgact caatgcgcgg accaacgaca tcccgacact
      181 gctcgcatgt ctccaggcct ccacatccga cacgcccacc ccgtcgtctt cgatgcgcag
      241 ctcgatcggg cccgaccccg tcaggctcaa ccgcacctcg ccccccgcgc gtcgatcgct
      301 cggaaacgcg tatttgaacg cgttggtgag taactcattg acgatcagtc cgcagggcag
      361 cgccaaatcc acgggcaagg ccagtggcgc gatctgatct cgcaccagta cgcgatgcga
      421 cacgcccacg taggttcgcg ttaggtttcg taccagcttc gagacgtact ctcgcatgtc
      481 cacgcgcgac ggcagcggat gttggtacaa ggactcgtgc aggatcgaga ttgaacgaac
      541 tcgattgccc gcctcctcca gcaccacgcg cgcttgtcgc tgatcggtgc gattcagctg
      601 gatgttgagc aagctcgaga tcacctgcag atggttcttg atccgatggt gaacctcccg
      661 cagcagcgta tctttctcct cgagtgccgc cttgagctgc gcgttctcgt cgttcaggcg
      721 ccgctcgcgc gagcgatcgc gcagcaccac gagcgcctcg tccgcgtggg cgctcgcgtt
      781 cattcgggct gttagccaaa acgcgccgcg cggcgcgtgc cattcgaagt cgaactcttg
      841 cggctggccg ctcgtcagcg cctcggccag atgcttacgc cataaggttg caacttctgg
      901 cggaagccat tcttccaatt tctgcccgac cgcttcgctc agatctggca ggatatccgc
      961 aggatcacag cggctacggc tcgggcgaaa cgccagtaga agcccagacg agctgaccct
     1021 cagcaaaggg tcggagagta cgtcgagcag cgggagcagg tcgttcgctc tcacactcgt
     1081 agcgcctgca ctcgcactgg ctacccagga atcagccacg aactctcctc gtcctcctct
     1141 ctcgaaaccc gaagagatta ccgcggattg attgtggcag tcatgcctca aactgaggct
     1201 tcttttcgcg tcggtttccg ccacctcgtg ggtgagacgg atctcagggg ggagtggtga
     1261 tcaatccgag ttcgagaccg agaaatccgc cgtcgaaaat ccgctcgatc gcgggatccg
     1321 agctcggcac acgccgaagg taagcgcctc cactcacccc taagttcgcg cgcagccgac
     1381 gcgacagccg aggctccgcg cggagagtcg cgccgacgcg agccgtccag ctttcgtgtt
     1441 gtctggccat gtcgtcgagc gacgatacac gcgagacgtg taacaacccg accccgagcc
     1501 aagctgcgac ggagaggtcc acgcgcggcg tgagcgaaaa gcggcgcgct ggaccgagag
     1561 tgagatccaa caccgaaacc caagggccgc tgtgcgcctg gccaccgctc gcggagaatg
     1621 cccaatccaa gcgcaacttg tgatgaatat tggtttcgaa cgcgagggtc gaaccaaagc
     1681 tcgtccactc acggctttgc gtcacgaaca agctggaacg cagcgcaaac ggcccttcca
     1741 ctgtgcggtt ggcgcgcacc tgagcgagct ccgcccaaag ccgttgccga tcggcgcgag
     1801 cacgctgttg ttgggctctc ttctgccgca gcccacgcgc gagctcggcg gcggcgagag
     1861 ccacgcggcg cgcggcgatt tgtaggttgc cgaggtcacc cgtcacgcga cggacgtcgt
     1921 ggaactcgcc cctttcccac agctcgacgc gtaccccgcc gttgggctct gccagcactc
     1981 gatagaagag cgtcggcatc tgccccgctc ttgccggcgg cacgtcgacg tcactcacct
     2041 cgagcgccac caaacgccga gtcgcgcgcg gatccaccca atgctcggcg gccgcgtcga
     2101 tctcaatcac cacgccgggc ccttgcgcga gcgctgggct cgacccaagc agcgtagtga
     2161 gcgcgaaaat cagcgcgcta aggagactgt tccgcgctcg gcgagcgagg ctcggcctga
     2221 gaatccgccg ccgcctcgcc acgggactca gaactgctcc cgttgttcgc ccccggagca
     2281 gcgccgccag gacttccatt cggcgccgct tgctcgcctt gaagcttacg cagctccaag
     2341 cggagctcgt gcagacgacg agcgtggggg aagtcctttt catactggtc gacgagctcg
     2401 gcggcgaaga cgagcttccc ctccgataga gctgcgtcta tctgacgggc ggccgcatct
     2461 tcggcgaaat caccggcagg cgacaagcgc cgatactgcg caaatgcttc ggctgccccc
     2521 gcgcgatcac cggcctgctc gagcaggttc ccgagcgtcc aggcggccaa cggcgcctcc
     2581 ggcacagagg gaaagctgga aagcagccgc cgcaaggcgc gaatcgcctg gtcacgctcc
     2641 ccggaagcgc gcgccacatc taccagcgtc atgagctggt cgggggaggc agagaacaaa
     2701 gcggcgtccc agccccccgc ccgctccagt tccgtacgtg cgcccgcaaa gtctccggca
     2761 tcggccttca gctgccaggc cttctgctgc caattcttct gttgggcacc ggcacccgca
     2821 gagctcgacg caggccccgc gtagccagga tcgcctgcca tcgcgggctc gagcggccga
     2881 ttataggcac tgtggctggg cgagcgcccg gcaaccggcc ggcggacgcc ggcggacgga
     2941 gggacagcgg gcgtgctctt cggcgcttcg accggcatgc gcacgccacc tgccgggctc
     3001 atcgcaacag gctgctctgc caagaatgcg ggctcgggca ctggggtcac taccgtttgt
     3061 cggcccaccc aaatcccaac cgcaaacgtt accgccccga gcgcgggcac gaagaataac
     3121 ggggcacgcg agcgacgctt ctcgcggcca gcctccgagc tcaagcgctg ccagacccga
     3181 tccaaacgtg ggtcacccgc gtagtcgcgc aacgcggtct tggatagttc ggacagcgga
     3241 tcgtcgatca tcggtcgtct ttgctcagca tctctttgct gagcggctct ttactgaatg
     3301 gcgccacgaa gtggcctgaa aggaagcgtt gcgcacgttg gatccggcgc ttggcggtcg
     3361 cgagcgaaca atccatcaac tcggccacgc tctcgagccg atggcgctcg atgaagcgca
     3421 acgtccacgc gatccgatcg cttgcgggca gagtctgcaa cagcgcgtaa atctgggcga
     3481 gcagcacgcg ggactcggtg tccgcgtcgg gagccgccac cgcatctaga tctacgggct
     3541 ccggcgcgat caggcccagg ctgcccaaca agcgccggcg ccgcaggcgt gtgcggacca
     3601 agcgcaccac gatgctgccg agccaggcgc gaaacgcgga ggcgtctttc agattcgcga
     3661 ggctatcgtg cgcgcgaatg aacgcgtcat gcacgatatc ctcgacgtcc cccgcgccgc
     3721 cttggatgcg gaccgcgagc cctagcgcgt acgcggcatg acggcgatac agagcttcga
     3781 acgcgtcgcg gtcgccatcc atcaccagcg cgaccacctg cgcatccgtc aggtcatcga
     3841 acaaactcgg ctgctctcgc cgcgcgcgct gaggccgctc ttcgggttgg ccatcacggc
     3901 caccgaccag acggagcttg ggcatatctc gaacgcgagc cacgaaagtc accttaaccc
     3961 ctgatgcatg gaccgcccgg ttcggctcat gcctacatgc ggctccccct gatctctcat
     4021 cgcggccagt agcaagcgaa agagtcgacc ggctcgcagg ctgcccgcgc tcagcgcatc
     4081 attcctgttc agctagaacg atcatgacca atcgtgcgcc ttttcgctcc aaagagtttc
     4141 gcgcgtgagc cgctttctga tacttcggcg tctttcacac ggtccgagcc cgagtcggtt
     4201 cggcaggagg aactcaagat gtcgattcgt aagtttgctg ccctgattcc ggttgctgtt
     4261 ctgatggctg cttgtggtgg tgaggcaacg ccggaagcga aggcacccga aggtggtgcg
     4321 gcagcggctg aagaagccaa gccggcagag ggcgatgcag cggccgcagc aggcgacaag
     4381 gccgctgaag gcgctgacaa ggccgccgaa ggcgccgaca aggccgccga cgaagccaag
     4441 ccggccgaga gcaagtgaat gccgaaccgg cagcatcgcg ggcctgaggg cgcgcatgct
     4501 gctggaaccc cagcttgggc agacccaaag ctcgggcgag tttgagagcg gccggagcgt
     4561 ggctccggcc gttcgctttt tgtgcgttgg tgctgcacgc tcgcgacagc gcggcccgct
     4621 ctttcgcgag caagacgcga ttgtcgagga ctaggctact tcaggcagct cgacgacgag
     4681 tcgcgtgcgg cccaccagca gctcgtcacc gcgcgtgagc tcctgctcgc cgcgaatgcg
     4741 cacgaacaca cccgtccgcg agccgagatc cgtcagcacg accgtgccgg cgtgttcttc
     4801 gattaggcaa tgttgctcac tgacgagcgg gtcatccggg aataccagat caccgattgc
     4861 cgatccgatc tgcagagtat tgccccgcgc cacgacgcag cacccgagtg ggccgccctc
     4921 caaaatctgc tgcacgcgga aagaagaggg ccacttcggt gacgaataga aataagtcgg
     4981 gtgctcatcg ggcccatcgt cgggcaccgg gttgcgctcc acgcgtagca gctgatcgcc
     5041 gaccacgaac tcgtccccca ggttgagctc tacgggtgtg cggattcgca agaatacgcc
     5101 gttgcctccg cccaaatcat ccagccacaa gcgctcgttt cgaaactcga gccgcgcttc
     5161 ctcggggtgg caaaagcgct cgcccggcag cgtcacctca cccttggcgc caatcagcgt
     5221 cgcgagccct cgaggctcgt agcgatcggg gccacccgtc aagctccgga gcacatgcaa
     5281 ggtgaagcga ctgcgcgcgc cacgttcact gcgcgcggct cgttcgggcc gttcgccacg
     5341 ttcactcgga acaacctccg agcgaggtgc gtcactcggg gccgcgtgtg ccgactccgc
     5401 gcgctgcttc ttctgactcg cgagcttgcg gatgttgtcg atgttcggtg gctctcgcaa
     5461 tacctcgagg ccgcccaccg cgcgacccgc caagatctcg tcgaagcggc cctcgcgcag
     5521 cgacatcacc atctccaggt gctgcgcctt catcaactgc cgcacgtact ccgctacatc
     5581 cgagcgcgat acctcggtcg cgtacgagcg cttgtgactc ttcaccacgc ggccgccgtc
     5641 cgcgaacaga tgagtgacga tgtgcggctg atccagcccg gaatcctcgg tctggacgtg
     5701 gaatacctcg ccctggaact tgatgttgtt gttgaagccc atctgcgccc ggcgaaacga
     5761 cgggcggcgg agctcggcct cgagccgcgc caactcatcc gaaccgaaac gcaccacgca
     5821 cgtatcgagc tcggagtgat cccccgccac gccttgcatg cgttcgaagg cttgctgcgc
     5881 taccggtgcg cttgggtcga tgcgcagggc ttcagccaat agctcttggc ccgctttgtc
     5941 gttgggaccc gtcgcagccc acgctgaaag cagcgccacg gcttcatcag ggcgctctcg
     6001 ctcaaccaac catttggcta ctcgcgcggc actcgcgcga cgatcgggtt cactgggggc
     6061 gggctcgggc atggacaccg tattgaatga gccaagcgct tccggcgcaa ggggggcctg
     6121 caaattccgt ggctttcgcc gctcgcccac cccgctggcc aaacaggtga gagcggggac
     6181 cacctcgggc cgtccaagca cgggcttgac agcccgtgct cagaaaatac cgcagtcttc
     6241 gttgcagaca cccagatcag aaggctcgca gcgttcattt ccgcaagtct ccaggacccc
     6301 gcaatcgaac gcgcacgtca gcgactcgaa gccgttacac acgcggtcgc cacagatctc
     6361 caaacccggg cagtctcgcg gacaggtcgt ctgattctca agccccgtac acttgccgtc
     6421 tccgcagccg gcgcagtcga agggacaaga cccgtccgcc tcgacgccgg tgcacaactc
     6481 gtcgccgcaa accgggcagt ccacggtgca gccgggtccg tcgaacgacg tacacacgcc
     6541 atcgccgcag cgattgcagt cgttcggaca ggtgcgcgca tcctcgtcca ggtcacacgt
     6601 accgttgccg cacgagcaat cgagccggca cgacgctctc gtttcgtcga acgtacaaag
     6661 accatcgcca cacgccgtac agtccgtcgg acaccagtcc accgactcga cgccttggca
     6721 aatcgaatct ccgcaggtgc cgcagtccgc actgcacgaa tctgcggtct cgagccacga
     6781 acagatccca tccccacaca ccgagcagtc actcgggcag gtgtctaggt tctcgaagag
     6841 atcggagcag atcccgtccc cacaggacgc gcagtcacgt gaacagttcg acgggttctc
     6901 gttgccttca cagaccaggt tgccgcacac gctacaatcg aacgcacacg tgaagacaga
     6961 ctcttcggcg tcacaggttc cgttgccgca aaagcagtcc ttgcacgagg ccgcactctc
     7021 gaacgactga cagaccccgt cgaagttgca ggcattgctg caatcccaac accagccaga
     7081 ctccatcggg gagcacacgc catcaccaca ctgcgggcag tctcgcgtgc actggcccac
     7141 ctcggcctgg ctacagtagc catcgccgca cgcgctgatt gggcaatcac cgcaccaagt
     7201 cgcgtcgttc ggctcgcaaa tgccgttgcc gcagcccagc tgtttacact cgatgcaacg
     7261 ccccacctcg ctcggctgac agatcatgtc gccgcattgc gggcagtccg aggcgcagtc
     7321 cttcttctcg gagggcgaac agatcccatc gccgcaaaag tggcagtcat cagcgcatga
     7381 ctttagggac tcgcccggct cacacagccc gttgccacac caggtagcgc cagaagatcc
     7441 gccagcgccg ctcgctccac ctacccagcc gcccgttcct ccaaagccac cgcctccaaa
     7501 tgggctgccc ccgaatgtac tgccgccggc cgtcttgccg cctccgttcg gcgcggctcc
     7561 cgctccggac gctccgccgc tggtcttgaa agtagtcccg ccggctccgc cccgcgccgt
     7621 gcccgaggtt ccgcctttga tggctccgcc gcttccgccc gttccacccg ttcccgcggg
     7681 gaggtcgtct ggaccttcga gcggcaactc ggcgcgccca cagcccagca tcaaaaccaa
     7741 accccagctc gcaaagaccg caagccgcga acaccgagcg agattcaatc cataagtcat
     7801 gccgataccc gccaaacgca actcgcattg tgcccgcccg cgggcgccag cgctagcgat
     7861 cggcgctcga aacggttcgg ctacggcact tcgtgaagcg agacaccttc ggtagagact
     7921 tccgtcgagc gtgccaagag atatgccggg ttatcgaaca ccgcgccgcc cttggcgcga
     7981 agcacgcact tcgacacgtg ttcctgagca acgcccgagt aattgcagta gggggcgccc
     8041 cgccactcca cgctgaccga cttggccgta ggcagcgcga ccttgagctc gtaactcccg
     8101 ggctccaagg aaaaatgaat cttgcgattg ccatcaatcg gcaatgcctc gggcgtcagc
     8161 ttgcgaacca cgtccacctt gaactcccgt gcagcctgcc cggcgcgcgc caagatcacg
     8221 gcgctcccca cgctcttggg caccaattcg gcacctcgag ccaccagcac ccccgtattg
     8281 cgcgacgtca gcacgacatc cacgccgtcc agctctgcgc cgcccttcgc gagcacccgc
     8341 accttgggct tgaacgcccc cgccgtaagc tccactcgac ccacgtcgct cgcgtccacc
     8401 cgatccacca agcggcagcg cagcgccacc tgccgtgtat tcgcccccca agtcagtgaa
     8461 acggcgccat ctccggagcg ctgacaagtc aggctgcccc gtggcgtgac gctggccaaa
     8521 ttagcaggtt ggacctgaaa cggatgttcc gcttcactgc tcgccccggc cccgtccgtc
     8581 cctttcacac gcacgaccac gcggacc
//
LOCUS       DASTKU010001067        12413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_211874, whole genome shotgun sequence.
ACCESSION   DASTKU010001067 DASTKU010000000
VERSION     DASTKU010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12413
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211874"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /gene="hemA"
                     /locus_tag="VFQ61_37925"
     CDS             complement(<1..720)
                     /gene="hemA"
                     /locus_tag="VFQ61_37925"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET9960350.1"
                     /translation="MVGLSHRTAPIEVRERLSLSRDTVPAFLRGLVAQGKVGEGLLVS
                     TCNRVELVAAGTSLNSDLKQVVESCLRAFEEHAPGISRHMYRHAGGEGVKHLFRVASS
                     LDSLVRGEPQILGQVKDAFEVARHAGTVGPILHRTLPRAIRAAKRVRHETLVGAGQVS
                     VPSVAVDLTRQIFGELRGRTVLLVGSGEMAEAVARLLVLGRTREKVEALAQAVGGEPR
                     LWPDLRAVLVEADVVITSTSAP"
     gene            complement(839..1645)
                     /gene="ccsA"
                     /locus_tag="VFQ61_37930"
     CDS             complement(839..1645)
                     /gene="ccsA"
                     /locus_tag="VFQ61_37930"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HET9960351.1"
                     /translation="MPWLASIAFYLGVIAYSAAATFFFLDLLRPAPHRAGAGPSLLAA
                     GAVSHAAHLVVAGWVSQTCPVGSLHFALGVSAIIAVAAFLAVRRRFRLDAVGSLIAPL
                     ALILLLSAEFMSLGPTISGELPRGLLLLHIAANVLGFGLFLLAGASGAVYVFEERRLK
                     RKRLGSLSKLPPLASLDRVAHRLLLVGFPLLTFGVVTGSMFLDQLGHASADTVLRSLL
                     GYLSWFLLALVLLLRRVSGFSGRRAAYGTLAGVTCVLLVVLVYVFRISPG"
     gene            complement(2001..3401)
                     /locus_tag="VFQ61_37935"
     CDS             complement(2001..3401)
                     /locus_tag="VFQ61_37935"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET9960352.1"
                     /translation="MKPAAFGWALAGVLAAAHGVVMPLAAAQPGPSPDAPAPNSGASN
                     PGAPSGNPGADTTTPGGTTTSTTTYVPFGVPSPGTDINAGLPSSSRPVRGDQSDTFDL
                     GRAGGGSTVVTGNKGSAGVFDAAPVREVPDVHLVQKGDTLWDLCNQYYRSPWEWPKVW
                     SYNPQIQNPHWIYPGDQLRLRHPNELSAMRGLAGNGANSRGRLLNPGQRVPPSTIFLR
                     DQGYLGDPSRDVWAEIVGAVEEQMLLSEGNHVYLIARPGVNLTPGQELTVFRDVRKPE
                     DVPGARSVPGRIVAVTGSVKVDQFDSKTRVARAVITESTDAIERGAKIGPVGRRFVVV
                     PPQKNTRNVEARVLTSLYPHVYLGQNQIVFLDHGSADGLAPGNTLLILRRGDAWRRSL
                     STASKMVRDRVRMDSPARVDVETTPLAGNPENFPDEAVAELRVLRADKQTAVALVTQS
                     RREVTPGDRAVAMIGR"
     gene            complement(3398..3814)
                     /locus_tag="VFQ61_37940"
     CDS             complement(3398..3814)
                     /locus_tag="VFQ61_37940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960353.1"
                     /translation="MRTLLLSCILSALALSCSSDRDAVEKRLAGLREDITRLQSQNDR
                     LAERVEVLESRPAPVSAPQPTEPRAERPPLKIVRLYPGEASPTAGEPDASTELPVDER
                     PDAPGNRPVIRLRGKQDASNDEPRDRTARRSATEQP"
     gene            4087..4878
                     /gene="pssA"
                     /locus_tag="VFQ61_37945"
     CDS             4087..4878
                     /gene="pssA"
                     /locus_tag="VFQ61_37945"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HET9960354.1"
                     /translation="MRKTLFVLPNLITLASVFCGFNAIRLVARDVPSENDVYRAAVLL
                     IFAMLFDLMDGRVARMTKTQSAFGLQIDSLADVISFGVAPALMLYKWVLYREPSAGIF
                     ASFLYVACAAIRLARFNVLSSSPTGVPVKPGKYIMGLPTPPACGILIALVVANHGMGG
                     ALGDEKYTWLLFGVTLTLSFLMISSVRFRSFKDLSLNTGTLLFALFVLASSAFVWNWL
                     KPQFVLVWLLSFYVFIGIAESLKALALRLLRGPSAPRDSLPPVQP"
     gene            4888..6768
                     /locus_tag="VFQ61_37950"
     CDS             4888..6768
                     /locus_tag="VFQ61_37950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET9960355.1"
                     /translation="MFARLRELLGSAISRHRDAARESAVAGGDGETCLEPPGLHSAWR
                     GTRRDWLGAQAGLAAGAVTACANQESGGTPIVPPDEPRVAIVGGGVAGLTCAYWLNQA
                     KIDVSVYEANSTRVGGRAFTGRELFSNSALTCEFGAEFVEPHHAAVWALAHELDVTIE
                     PRQTAGAGRVDFFWLEGKQVPLATLLAQLAAVSDRISRALLDANADAGRFFLLDNTSL
                     GNWLSENIPARDYPELSLALGLAYRGEFGLDVEQLSALNLLHLVSAAVKPQDPKPSDP
                     AVQLSYGLFGQAQSTYRLRGGADTLAQRLAEGLLGRVKLDSQLISIERSGQQHSLTFQ
                     SRAGGGFAVAADRVVLALPFSTLRQVPLDELALPADVRDVILTLGYGSHCRTVGAFDQ
                     ILWRGQGYVRGSVASDLPFEQAADANISSEGTREGLLGNTVSGSLASAARDADVQTSY
                     RAALDALQAIFPGIKEAYQTESARRVHWEKLPFSRGSRSCRKPGQWASRAVAQTAQGH
                     LRFCGEHCSIDFPGTLEGAAETGALVAGQLILELGHKLPPALQQLLQLKSRVLQPCCD
                     LQETGSSWPVVSSSTTEPGSKSDPNAAYDVLPALDGTKLGFLERLSAVLESHTAFTRQ
                     FM"
     gene            7173..11270
                     /locus_tag="VFQ61_37955"
     CDS             7173..11270
                     /locus_tag="VFQ61_37955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HET9960356.1"
                     /translation="MSCVSRTALAGRRWFASVVCLASSIFSTPARALNPRASVEQYSV
                     ESWGNHDGLPEGGVLAIQETRDGYLWLGTTQGLQRFDGARFVGFEERQRGLPQYSFVR
                     DLLETRDGKVWAAAVGGVARFDGSRFSFFDERRGLPHPFMYALAEAPDGTVFAGSGGN
                     GVYSLTDAGARLHAAYTENPKLPGKINDLVVSRDGSLWAATDHGVLNFAREVRVYDVA
                     NGLPSSSVNALRFDRDGALLAGTSAGLARLDGERFVTELSLGENSVSSLLYDHDENLW
                     VGTQAGDLFRLRGRQASLAIDSSRTSNGGVYALAESRDGSLWVGTGSGLLRYHDGAFL
                     TLGRREGFAEDNLLTLAPRAEGGLWVLDATGSLWIHDRGSVSRVSAPGAVHGEGMLGM
                     LETAPNDVWIAGDLLYRFHNGTWQTYASPGGELSVMIKDGAGLLLAQTGSDGKSSLWR
                     FEAGQFQLVPVAVPLTHVQRMYRDRSARLWISTGRGLVRIEGQKTKVFGARDGLPNDI
                     VYGLEEDETGALWVATRSGLARIRDDRVANFEQVTALPRRSPVHLTSDGLGYLWVTAD
                     DGVHRIRKQDLDAIADGKSVDPVVRKFTLRDGLRSLHVSWRAGAQARATDGRLYYATR
                     RGLSSVEPAAVEEHASRPEPRVEELWVDGKPVEPRDGLSFSKSPERLEIRYTSPSLVH
                     AQHLRFRFRLVGYDEGWVDVGKRRMAFYTTLPPGDYEFRVAAREDTGSYGPEATLRFR
                     VEPRFFQTTWARILLSLAAGLALYGAYRLRTRQLRGREQLLLRKVNERTAELNREVFE
                     RRLAEQQVRKLNEGLEARVLDRTLRLENANTALEKDIQERKRAEAALFEEKERLAVTL
                     RHIAEGVLTSDAEGRVVLMNPVAERLTGFNLQEAALKPVSEVLRLADRFTLQPAPPTT
                     SWPPEASDSRLLTRVALIPRQGSPVLLDVAIAKIRDQSGTPVGSVIAFRDVTEKTRAE
                     EQLQKAQKLEAIGVLAGGIAHDFNNLLTGLFGHLDLARAALPPGSNASVWLEDAVDVL
                     SNARGLARQLLTFSSGGRPSTESHSLAELLRKSTRFVLSGSSVSAELELPEDLSPCDI
                     DPLQIRQAIDNLLLNARQAMPFGGHIRLSARNLEVEELEHDQPVKRRYVELAISDDGP
                     GIPPEIRSRIFEPFFTTKSTGTGLGLATVQSIIAQHGGSITFDSELRAGTTFRIRLRA
                     ADRAPVGEHRTPPLREVRQGHARILVMDDEPLIRKLARVALGQAGYDVVLASEGGEAV
                     KLFMQAHGEGRPFDLVVLDLTVAGGKGGLETLLELRGFSPNVRAMASSGYSSGRVFSD
                     PKAFGFIGVLPKPYSLVDLTTAVESALSSEPPELAAAPSSSAFVA"
     gene            11351..>12413
                     /locus_tag="VFQ61_37960"
     CDS             11351..>12413
                     /locus_tag="VFQ61_37960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET9960357.1"
                     /translation="MARAPTACHARAPAVATLLEVSDLAFGYSNDKLFSKVTFRLNSG
                     DRIALVAPNGAGKSTLLRVIAGELQADQGTVLRKPSVSFGYYRQSHEVIHEGRVLDVL
                     MEGFGDALRVRQELRNAEAAAASGGEAELTRLASALDRYHQTGADRLEQRVEMIATRL
                     GFSKADLERDVKTLSGGERGRLSLGVVLATEPELLVLDEPTNHLDLESIRWLEGHLRD
                     QRGGLLVVSHDRAFLDAVCPATMELGRTSFRVFPLPYTQYHEEREVELERERRLAEEQ
                     ANFVAKTEDFIRKNMAGQKTKQAQSRRKMLDKMERIDRPEDVWARAERVRFRFADVPR
                     SGDIVLEAKGLGAERGGVHL"
BASE COUNT         2309 a         4051 c         3908 g         2145 t
ORIGIN      
        1 cggcgcgctc gtgctcgtaa tcacgacgtc cgcttcgacc agcacggccc gcaaatccgg
       61 ccacagccgc ggttccccgc ccactgcttg cgccagcgcc tccaccttct cgcgcgttcg
      121 tccgagcacg agcaagcgag ccaccgcctc cgccatctcg cccgagccga cgagcagcac
      181 cgtgcgcccg cgcagctcac cgaaaatctg ccgcgtcaaa tcgacggcca cgctcggcac
      241 cgagacttgc ccggctccca ccaacgtctc gtgccgaacc cgttttgcgg cgcggatcgc
      301 gcggggcaac gtgcggtgca agatcggccc caccgtgccc gcgtgccgcg ccacctcgaa
      361 ggcgtccttc acctggccca gaatctgggg ctcgcctcgt accagcgaat ccagcgacga
      421 agccactcgg aacaggtgct tgacgccctc cccgcccgcg tgccggtaca tatgccgcga
      481 gatgcccggc gcatgctctt cgaacgcacg caaacagctc tccacgacct gtttgaggtc
      541 ggagtttagg gacgtgcccg cggcgaccaa ctccacccga ttacaagtcg agacgagcag
      601 cccttctccg actttgccct gagcgacgag gccgcgcaga aaagccggca ccgtatcgcg
      661 agacaaactc agccgctccc gcacctcgat cggcgccgtg cgatgagaga gccctaccac
      721 gacgatcaag gctcacccgc catgagcaaa tccgggcccg cgacgtggga ccgtcgcccg
      781 tggggctcgc tcacgccaaa atcgaaggcc tgaaatcctg ctgttcgcgt caagagactc
      841 aaccgggaga gatccgaaac acgtacacca gcacgaccaa caacacgcac gtcacgcccg
      901 ccagagttcc ataggccgcg cgccggccac tgaacccgct cacacgccgc agcaacagca
      961 ccaatgcgag cagaaaccag ctcaagtatc cgagcagtga ccgcagcacg gtatcggcgc
     1021 tcgcgtgccc gagctgatcc aggaacatcg agccggtcac caccccgaac gtgagcaaag
     1081 ggaacccgac caacagcaag cgatgcgcga cccgatccaa gctcgccagc ggaggcagct
     1141 tggagagcga ccccaaacgc tttcgcttga gcctgcgctc ctcgaacacg taaaccgcgc
     1201 ccgaagcacc cgcgagcaag aacagcccga agcccagcac gttcgccgcg atgtgcagta
     1261 gcaacaagcc gcgcggcagc tcccccgaaa tggtgggacc gagcgacatg aactccgcgc
     1321 tcaacagcag aatcagcgcc agcggcgcga tcagcgaacc caccgcgtcc aagcgaaagc
     1381 ggcgccttac cgccaagaag gccgccaccg cgatgattgc actcaccccg agcgcgaagt
     1441 gaagcgatcc aaccgggcag gtttgcgaga cccaacccgc caccaccaag tgcgccgcat
     1501 gcgagacggc accggccgcg agcaagctcg gccccgctcc cgcgcgatgc ggcgcgggcc
     1561 tcaacaaatc caagaagaag aacgtcgccg ccgcggagta agcgatcact cccagataaa
     1621 acgcgatgct agcgagccac ggcattcagg cggttccaga cgaaagtgcg gcagggaggc
     1681 tagcatcccc gccgccgttg gacgaagcca gccccgaaac attgcgccaa ccgcccgttc
     1741 aatcgggcgc cgcgtgagcg gactcgacag tcgaaccggc cgaattcggc ccaattcgcg
     1801 gttagctccg caacccgaac ggcgaaatcc ggctcggcaa agcccctatt tggggcccgg
     1861 ccaggtaacg tgcgcgcctc gagtccaagc ccccgcccgg tcaccgttcg cggccaggcg
     1921 cccgccgcag ccgcgctcgg gccacttcgt aacgcgccga ggctctctcc agaccacgag
     1981 tcacgcgcgg atacgcttca tcaccttcca atcatggcca cggcacgatc acccggggtc
     2041 acttcacggc gtgattgcgt gacgagagcc acggcggttt gtttgtctgc acgcagcaca
     2101 cgcagctcgg ccaccgcttc gtccggaaag ttctcggggt tccccgccag aggcgtagtc
     2161 tcgacgtcca cgcgagcggg tgaatccatg cgcacgcggt cgcgcaccat tttggaagcg
     2221 gtggagaggc tccggcgcca ggcgtctcca cgtcgcaaga tcaagagtgt atttccaggc
     2281 gcgagcccgt ccgcactgcc gtgatccaaa aacacgatct ggttctgacc gagatacacg
     2341 tgcggataga ggctggtgag cacgcgcgcc tccacgtttc gtgtgttctt ttgcggcggc
     2401 acgaccacga aacgccgccc gacgggccca atcttcgcgc cccgctcgat ggcatcggtc
     2461 gattcggtga tcacagcgcg cgccacacgc gtcttcgaat cgaactgatc gactttgacg
     2521 ctcccggtga cggccacgat ccgcccgggc acactgcgcg ccccgggcac gtcttcgggc
     2581 ttacgcacgt cgcgaaacac cgtcaactct tgccccggag tgaggttcac gcccggacgt
     2641 gcgatcaaat agacgtggtt gccttcggag agcagcatct gctcctctac ggcgcccacg
     2701 atttccgccc aaacgtcgcg gctcgggtca cccaggtagc cctggtcccg caagaagatc
     2761 gtcgaaggtg gcactcgctg ccccggattc aacaggcggc cgcggctgtt tgcgccgttc
     2821 cccgccaggc cccgcatcgc ggagagctcg ttcgggtgcc ggagccgaag ttgatccccc
     2881 ggataaatcc aatgcggatt ctggatctgg ggattgtaac tccagacctt cggccactcc
     2941 cacggactcc ggtagtattg attgcacagg tcccacagcg tgtccccctt ctgcacgagg
     3001 tgcacatcgg gaacctcgcg aacgggagcc gcgtcgaata cgccggcaga acctttgttg
     3061 ccagtcacta cggtggagcc gccgcccgca cgccccaagt cgaaggtgtc agactggtcg
     3121 ccgcgcacgg gccgcgagct agacggtaat cctgcattga tatcggtgcc cggcgaggga
     3181 acgccgaacg gcacatacgt ggtggtgctc gttgtcgtgc ccccgggggt ggtcgtatca
     3241 gcgccagggt tgccgctggg agcacccgga ttggaggcac cagaattggg agcgggcgcg
     3301 tcagggctcg ggccgggctg cgccgcggca agtggcatca caaccccgtg ggccgccgca
     3361 agaactcctg ccaaagccca cccgaaagct gctggtttca tggctgctcc gtcgcactgc
     3421 gccgggctgt acggtcacgc ggctcgtcgt tgctcgcgtc ttgtttcccg cgcagacgaa
     3481 tcaccgggcg gttacccggg gcgtcggggc gctcgtccac gggcaactcc gtgcttgcgt
     3541 cgggttcacc cgcggttggg ctcgcctctc cgggatacag ccgaacaatc ttgagcggcg
     3601 gtcgctcggc gcggggctcg gtgggctgag gagcgctcac cggagcgggt cggctctcga
     3661 gcacttcgac ccgctctgcg agtcgatcgt tttgggactg caaacgcgta atatcctcac
     3721 gcagacccgc aagccgcttc tcgacggcgt ctcgatcact cgagcaactc aacgcgagcg
     3781 cgctcaagat gcaacttagc agtaaggtcc gcattggcag ggaatgttat cgagagctta
     3841 cgcagatagg caaaaaacca gctcgggcaa taacaacggc cgacaacctc gcgaacgcgc
     3901 cccgcattaa tgggcattcg cacacggcac atggccgtag ctccaggtgc tccactcgca
     3961 tacagaccaa gcaaacggca gccgacccat gagccggcca gccgagcgct ggacggagga
     4021 gtcgaccttg gttaccctcg agccgtgcgc attcggaggc ctcgtcgcgt tcgccctcgt
     4081 gtcgatttgc ggaagacgtt gttcgtcttg ccgaatctga tcacgctcgc gagcgtgttc
     4141 tgtggcttca acgccattcg cttggtcgct cgagacgtac cgagcgaaaa cgacgtgtat
     4201 cgcgcggcgg tgctgctcat ctttgccatg ttgttcgatc tgatggatgg ccgcgtagcg
     4261 cggatgacca agacgcaaag cgcgtttggc ctgcagatcg actccctcgc cgacgtgatc
     4321 tcgttcggcg tagccccggc cttgatgctg tacaaatggg tgctctaccg cgagccgagt
     4381 gcgggcatct tcgcgtcctt cctgtacgtg gcttgcgcgg caatccggct cgcgcgcttc
     4441 aacgtgctct cttcgtctcc cactggtgtg cccgtaaaac ccggtaagta catcatgggt
     4501 ctgcctaccc ccccggcctg cggcattttg atcgcgctgg tcgtggccaa tcacggcatg
     4561 gggggcgctc tgggagacga gaagtatacg tggctgctgt tcggagtgac cctcacgctg
     4621 agcttcttga tgatctcgag cgttaggttc cggtcgttca aggacttgtc cctgaacacg
     4681 gggacgctgc ttttcgcgct cttcgtgttg gcatcgagcg ccttcgtttg gaactggctc
     4741 aagccgcagt tcgtgctggt ctggttgcta tcgttctacg ttttcatcgg catcgcagag
     4801 tcgttgaagg ccttggcctt gcgcttgttg cgtggtcctt cggcgccgcg cgattcgttg
     4861 ccccccgtcc agccctgaag gtttgcggtg ttcgccagat tgcgagagct gctcgggtcg
     4921 gccatctcga ggcaccgcga tgcggcccgt gaaagcgccg tggccggtgg tgatggagaa
     4981 acgtgcctcg aaccaccagg cctgcactcg gcatggcgtg gcacgcgccg cgactggctc
     5041 ggagcgcagg cgggattggc ggcgggcgcc gtaactgcct gtgccaacca agagagtggc
     5101 ggaacgccca tcgtccctcc ggatgaaccg cgcgtggcga tcgtgggtgg cggagtagcc
     5161 gggctcacct gtgcatattg gctgaaccag gccaaaatcg acgtctcggt atacgaagcc
     5221 aacagcacga gagtcggcgg ccgcgccttc acgggacgag agctgttctc gaactcggcg
     5281 ctgacctgtg agttcggtgc cgagttcgtc gaacctcacc acgcggcagt gtgggcgctc
     5341 gcgcacgaac tcgatgtaac gatcgagcca cgtcagaccg ccggggcggg ccgcgtggat
     5401 ttcttttggc tcgagggcaa gcaagttccc ctggcgaccc tgctcgcgca gctcgctgcc
     5461 gtcagtgatc gaatcagccg tgccttgctc gatgcaaacg cggatgcagg caggtttttc
     5521 ctgctcgaca atacgtcact cggcaattgg ctatccgaga acattcctgc gcgagattac
     5581 cctgaactga gcttggctct cggcctcgcc tatcggggcg agttcgggct cgacgtcgaa
     5641 cagctatccg ctctcaattt gctgcaccta gtgtccgcag cggtaaagcc ccaagatccc
     5701 aagccgagcg atcctgcggt tcagttgagt tatgggctgt tcggacaagc acaatccact
     5761 taccggctgc ggggcggcgc ggatacgctc gcgcagcgcc tcgcggaggg cttacttggg
     5821 cgcgtcaaac tagatagcca gctgatctcg attgaaagat cgggacagca acactcgctc
     5881 acgtttcagt cgcgcgccgg cggcggtttc gcggtagcag ccgatcgcgt ggttctggcg
     5941 ttgccgttca gcacactgcg acaagtgccg ctcgatgaat tggcgctgcc ggcggacgta
     6001 cgtgacgtga ttctgacgct aggttacggc tcacactgcc gcacggtggg tgccttcgac
     6061 cagattttgt ggcggggaca aggctacgtt cgcggtagcg tcgcgagcga tcttccattc
     6121 gaacaagccg cagacgcgaa catctcgagt gaggggacgc gcgaaggctt actcgggaat
     6181 acagtgtcgg gctcgctcgc atcggcggct cgcgacgccg acgtgcaaac cagttaccgt
     6241 gccgccctgg acgcgttgca agccattttt ccgggcatca aagaggccta tcagaccgag
     6301 agcgcgcgcc gggtgcactg ggaaaagctg ccattttctc gcggaagccg ctcttgccga
     6361 aagccgggcc agtgggcgag ccgagcggtt gcccaaacgg cccagggcca tttgaggttc
     6421 tgcggggaac actgctcaat cgatttccca ggcactctcg agggggccgc agaaacaggc
     6481 gctctcgttg cgggacagct catcctcgag ctaggccaca agctgccgcc ggccctccag
     6541 cagctcttac agttgaagag ccgcgttctc cagccatgtt gtgacctaca ggagaccgga
     6601 tcgagctggc ccgtcgtgag ctccagcact acggagcccg ggtccaagtc ggatcccaat
     6661 gccgcctacg acgtgctccc cgcactggat ggcaccaaac tgggattcct ggaacgcttg
     6721 agcgccgttc tcgaaagcca caccgcattc acacggcagt tcatgtaggc agcagggctc
     6781 cggagccata tgccatcgag tttgtgggga ccgcgcctga gcgtaccaag ccacgagcgt
     6841 aaacccacag gtatacgccg ctaaacagtt cgccgccaag cagtgtgggg gaccgcgaac
     6901 gcccgaataa aagctctcgc cgctgttcgc gattggcggc ggatcctgta gagtgatgag
     6961 tttgaagttg cttgcgctcg agcatcgacg agcccaccca cagccgccca gaccccggca
     7021 cacgcagcgc cagcactacc cggctccaat tgacgagcta acgagtcaaa cggcccgata
     7081 acccctgagc gctaaacaag gttgtagcgc aaaaccagca cagtttcttg aaccctgatt
     7141 cgccttcctc gatcgcggcg tttcgcaggc gcgtgagctg cgtatctcgc acagcactcg
     7201 ccgggcggcg ctggtttgcg tccgtggtgt gcctcgcgtc gagcatcttc agcacgccgg
     7261 cccgagcctt gaacccgcgc gcgagtgtgg agcagtactc ggtcgagagc tggggcaacc
     7321 acgacgggtt gcccgaaggt ggggtgttgg cgattcagga aacgcgggac ggctacttgt
     7381 ggctcggcac cacgcagggg ttacaacgct tcgacggagc acgattcgtc ggcttcgaag
     7441 aaagacaacg cggccttccc cagtacagct tcgtccgtga tctactcgag acccgagatg
     7501 gaaaggtttg ggccgccgcg gttggaggcg tcgcccgctt cgacggctcc cgattcagtt
     7561 tcttcgacga gcgccgcggg ctgcctcacc cgttcatgta cgcactggcg gaagcaccag
     7621 atggcactgt gttcgccggc agtggcggga acggcgttta ctcactcacg gacgcgggcg
     7681 cgcggctcca cgccgcctac acggaaaacc ccaagctccc gggcaaaatc aacgatctag
     7741 tggtgtcccg ggacggctct ctgtgggccg cgacggacca cggcgtattg aactttgcgc
     7801 gcgaggtgcg cgtctacgac gttgcgaacg gcttaccgtc gagcagtgtc aacgccctgc
     7861 gtttcgatcg cgacggagcg ctgctcgcag gcacctccgc gggcctcgcc cgcctggacg
     7921 gggagcgctt cgtgacggag ctgagcctcg gtgagaacag cgtgagtagc ctgctctacg
     7981 atcacgacga aaacctctgg gtcggcacgc aagccgggga cctcttccgc ttgcgtgggc
     8041 gccaggcgag cctcgcgatc gatagcagtc gaaccagcaa cggcggagtg tacgcgctgg
     8101 cggaaagccg cgatggctcg ctttgggttg gcacgggcag cggattgctt cgctaccacg
     8161 atggcgcctt cctaaccttg ggccgacgtg agggatttgc agaagacaac ctgctcacgc
     8221 tggctcctcg cgcagaaggc gggctgtggg tgctagacgc cacggggtcg ctctggattc
     8281 acgatcgtgg atcggtatcc cgggtgtccg cgccgggcgc ggtgcacggc gagggtatgt
     8341 tgggcatgct cgagaccgct cccaacgacg tttggatcgc gggtgacctg ctgtaccgct
     8401 ttcacaacgg gacttggcaa acctacgcca gccccggcgg cgagctctcg gtaatgatca
     8461 aagacggcgc aggtttgctg ctcgcgcaaa ccggaagcga cgggaagagc agcctgtggc
     8521 gcttcgaagc cggtcaattc cagctcgttc ccgtcgcggt accgctcacc cacgtgcaga
     8581 ggatgtatcg tgatcgcagc gcccgcttgt ggatcagcac cggccgcgga ttggtgcgaa
     8641 tcgaaggcca aaaaaccaaa gtgttcggcg cgcgcgacgg gctaccgaac gacatcgtgt
     8701 acgggctcga agaagacgaa accggcgccc tctgggtggc gacacgcagc ggcctagcgc
     8761 gcatccggga cgatcgggtc gcgaacttcg agcaagttac ggcgttgccg cgccggtccc
     8821 ccgtacatct gacgtcggac ggcttgggtt acctgtgggt gaccgctgac gatggcgttc
     8881 accgcatccg taaacaggat ctcgatgcca tcgccgatgg gaagagcgtg gatccggtag
     8941 tccggaagtt caccttgcgt gatggtttgc gcagccttca cgtctcctgg cgtgccgggg
     9001 cgcaagcccg agcgaccgac ggacgcctct actacgcgac cagacgcggg ctctcgagcg
     9061 tcgagcccgc cgccgtcgag gagcacgcgt cacggcccga gccgcgcgtc gaagaacttt
     9121 gggtcgacgg gaaacccgtg gagccccgcg atgggctgtc attttccaag agccccgagc
     9181 gcctcgagat ccgatacacc agcccgagcc tggtacacgc gcagcacctg cggttccgct
     9241 ttcgtctcgt cggctacgac gagggttggg tggacgtcgg caagcgacgc atggccttct
     9301 atacgacgct ccccccagga gattacgagt ttcgcgtcgc ggcccgtgaa gacaccggca
     9361 gctatgggcc ggaggcgacg ctgcggttcc gcgtcgaacc acgctttttc caaaccacct
     9421 gggcccgtat cctgctctcg ctagcggcag gcctcgccct ttacggggcg tatcggctgc
     9481 gaactcgcca gctccgcggg cgagaacagt tgttgttgcg caaggtgaat gagcggacgg
     9541 cggagctgaa tcgcgaagtc ttcgagcgac gcctggcaga acagcaggtc cgaaagctca
     9601 acgaaggatt ggaggccaga gttctcgatc gcacgttgcg actcgaaaac gccaacaccg
     9661 cgctcgagaa agacatccag gaacgcaagc gagccgaggc cgcgttgttc gaggagaagg
     9721 aacgcttggc ggtcacgctg cgccacatcg cagagggcgt gctgacgtcc gacgcagagg
     9781 ggcgtgtcgt gctgatgaat ccggtggcgg agaggttgac gggattcaac ttgcaagagg
     9841 ccgcactcaa gcccgtttcc gaggtcttgc gtttggccga tcgcttcacg ttgcagcctg
     9901 cgccgccgac gacgagttgg cctcccgaag ccagcgattc acgactgctc acgcgcgtcg
     9961 cgctcattcc aaggcagggc agcccggttc tgctggatgt ggcgatcgcc aagattcgag
    10021 accagtcggg aacgcccgtc ggttcggtga tcgccttccg cgacgtgacg gagaaaactc
    10081 gtgccgaaga gcagctccaa aaggcgcaaa agctcgaggc cattggcgta ctggcgggcg
    10141 gcatcgcgca cgacttcaac aatctattga ccgggttgtt cggccatttg gatttggcgc
    10201 gggcggcctt gccccctggt tcgaacgcct cggtttggct cgaagacgcg gtggacgtgc
    10261 tctcgaatgc gcgcgggctc gcccgtcagc tcctgacctt ctcgtccggc ggcaggccgt
    10321 cgaccgagtc gcattcgttg gccgaacttc tgcgcaagag cacccgcttc gtgctctcgg
    10381 gatccagcgt atccgcagag ctggagttgc cggaggactt gtcgccctgt gacatcgatc
    10441 cgctgcagat caggcaggcc atcgacaact tgctgctcaa cgcccgacag gccatgccct
    10501 ttggtggcca catccgactg agcgcgcgca acttggaggt ggaggagctc gagcacgatc
    10561 agccggtgaa gcgccgctac gtcgagctcg ccatcagcga cgacggcccc ggtattccgc
    10621 cggagattcg ctcgcgcatc ttcgagccct tcttcaccac gaaatctacg ggcaccggtc
    10681 tgggcctagc caccgtacag tcaatcatcg cccagcacgg gggctcgatc accttcgact
    10741 ccgagcttcg cgccggcacc acctttcgaa tccgcctgcg cgcggcagat cgcgctcccg
    10801 tcggtgagca ccgcacgccg ccgctgcgtg aagtgcgcca agggcacgcg cgcatcctgg
    10861 tgatggacga cgagccactg attcgaaaac tcgcccgagt cgcgctcggg caagcgggct
    10921 acgacgtagt gctggcgagc gaaggcggcg aggcggtcaa gctgttcatg caagcgcacg
    10981 gcgagggtcg acccttcgat ctcgtggtct tggacctcac ggtcgcgggg ggcaaaggcg
    11041 ggctggagac gctgctggag ctgcgcggtt tctcgccaaa cgtgcgagcg atggcttcga
    11101 gcggctactc gagcggccga gtcttctcgg atcccaaggc cttcggtttc attggtgtgc
    11161 taccgaagcc ctacagcctc gtggacctta cgaccgccgt cgaatcggcg ctatctagcg
    11221 agcctccgga gctcgcggcg gcgccgagtt ccagcgcttt cgtcgcctga aactggcgtg
    11281 ctccgcggcg cggagctgcg cagccagacg gggcgaacga cctttcccgg ttacggtgaa
    11341 gcaaggtgca atggctcgtg cgccgacggc gtgtcacgct cgcgcccctg ccgtggcaac
    11401 gctcctcgaa gtttcggacc tcgcgttcgg gtattccaac gacaagctgt tcagcaaagt
    11461 gacgtttcgc ctcaactcgg gcgaccgcat cgcgctcgtc gctccaaacg gagccggtaa
    11521 gagcactttg ctgcgggtga tcgccggaga gctgcaagcc gaccaaggga cggtgctgcg
    11581 caagcccagc gtgagcttcg gctattatcg ccagagtcac gaggtgatcc acgaagggcg
    11641 cgtgcttgac gtgctgatgg aaggcttcgg ggacgcattg cgcgtgcgcc aggagctgag
    11701 aaatgcggag gcggccgcgg catccggagg cgaagcggag ctcacccggc tcgccagcgc
    11761 gctcgatcgc tatcatcaaa ccggcgccga tcgcctcgaa caaagggtcg aaatgatcgc
    11821 gacccgcctc ggtttcagca aggcggactt ggagcgcgac gtaaagaccc tgtcgggagg
    11881 agaacgcggt cgtctgtcgc tcggcgtggt gctcgccacc gagccggagt tgctcgtgct
    11941 cgacgaaccc accaatcact tggacttgga gagcattcgt tggctcgagg ggcacctgcg
    12001 tgaccaacgc ggtgggttac tggtggtctc ccacgaccgc gcattcttgg atgccgtttg
    12061 cccggctacg atggaactcg ggcgcactag cttccgtgtg ttccccctgc cctacaccca
    12121 atatcacgag gagcgcgaag tagaactcga acgcgagcgg cgtttagccg aagaacaagc
    12181 caatttcgtg gccaagaccg aggacttcat ccgcaagaac atggcgggac agaagaccaa
    12241 gcaagcccag tctcgccgaa aaatgctgga caagatggag cgcatcgatc ggccggagga
    12301 cgtctgggct cgagccgagc gcgtccgctt ccgtttcgct gacgtacccc gcagcggaga
    12361 catcgtgctc gaagccaaag gtctaggcgc ggagcgcggc ggagtgcacc tct
//
LOCUS       DASTKU010001068         6421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_657509, whole genome shotgun sequence.
ACCESSION   DASTKU010001068 DASTKU010000000
VERSION     DASTKU010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657509"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(434..1957)
                     /locus_tag="VFQ61_37965"
     CDS             complement(434..1957)
                     /locus_tag="VFQ61_37965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960358.1"
                     /translation="MNTLFALRLALLATFALGIALIMLRPEQAWQKAREFLLEPTHPA
                     NLGLLRAVVFGTVFYDACHSQAWWYATLPKEFVSPPRGWEWLGAWFESWLPYVKPAQT
                     LLCVCAALASLGVLARVTTKLAAILAVLVLGVPAFFFKVSHGLHMPVLLALVLASSRA
                     GDGLAIDVWIRQYRGQPAPSPGPAYTIPVRLCWLILGTMYLFPGVWKLWDTGDQWLNG
                     TKLQVEMAKKWAQSPEFVPALRVDHSKWLLAIGGAFTLIFEIGFFFAILWRPTRLLGA
                     LAAALFHTGVGLTMGIWFSVFFPLIVFIDFPAVLRLWPFRYAAPILIAAFETARRRLS
                     LRPQPAPALDSNRSHYSASAPPLLVGLLLLTSMLYAGLVPVDSWPISVYPLFNSRVGK
                     APERGSWLEFALSGPDEQRRELGRLTFAHLQDNAEAYRIAREVAPGFDPKYRPKKMSE
                     EDKQRYIDLIARFVRESVGETGDAETLLIYRREFLLDPELRGTRSFARDLVAEVPLQ"
     gene            2230..3318
                     /locus_tag="VFQ61_37970"
     CDS             2230..3318
                     /locus_tag="VFQ61_37970"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9960359.1"
                     /translation="MVFDWSRYTGNIAWLAQRTIYLARHGSHAYGTNIATSDEDFRGV
                     AIAPREFYLGGLHRFDQAECKSPDLTIFDLRKFVGLAAQANPNVIEVLFVENADRLQV
                     TAAGERLLSARELFVTRRIRHTFSGYAASQLKRIRAHRRWLMNPPAAPPERKAFGLPD
                     TTAIPGDQLLAAEAAIRAQLDAWSADYLDELDPVIRETITGKMTRHLAEMGVAMNEDL
                     WPGAARVIGLDDNFIEYLAREKKYNAAKKEWENYQTWRRERNPARAVLEAKFGIDTKH
                     AMHLIRLLRMAREILETGRVIVRRPDAEELLSIRNGAWSYERIVDWAEQEDAALQEVA
                     AKSTLPKAPDVEAIDRLCVELIGEAIGC"
     gene            3444..4394
                     /locus_tag="VFQ61_37975"
     CDS             3444..4394
                     /locus_tag="VFQ61_37975"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET9960360.1"
                     /translation="MRLGQVLSVVLFAACEAHAPSVGSRDDPRAVPSSTVPSSTTPVV
                     EPALRVRWLGGPTATLERSGLRVLTDPMLGPREDVAFRLPKHPSSGVLDAPVARYTSP
                     PAFDMQGLTAILISHTHNDHFDATAQRTLPKSLPVVVAKAGADKVRAAGFADVRPLDW
                     SESLTLEHAGTNLKITAVPAHHAHDAGLDSELGRGNGYVLEWRDSRGVCRVYWTGDAV
                     LSDDSRGLAEKYGPIDLLLPHLGGVGGDGGLGLRTMNAAETLGLVERVNPALVIPIHH
                     TTFAHYREPIEALEQRAGEAGLAKSFHFLREGESFDLQRR"
     gene            complement(4476..5291)
                     /locus_tag="VFQ61_37980"
     CDS             complement(4476..5291)
                     /locus_tag="VFQ61_37980"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HET9960361.1"
                     /translation="MMIRMGGIILSGFGLVCALGGCSAEYAGSELESTESTSSVAKTD
                     QPLLVCAAGVFLAGQAAFAGGPDNDVVSGTMGANVLNGGNCNDILSGLDGNDTLNGGD
                     GDDTLNGGDGVDTLTGGNGDDNMNGGNQGDTLWGGDGVDTLHGDDGADELHGGNGADL
                     IYGDNGADTVDGGDDADNLHGGDGDDTVLGGNGADTLFGENGDDTLRGGDGNDVLYGG
                     DGADTLNGGNDDDVLFACDGAADTVQGGPGTDVCYGDCGTDTFIGCETINCCP"
     gene            complement(5317..5988)
                     /locus_tag="VFQ61_37985"
     CDS             complement(5317..5988)
                     /locus_tag="VFQ61_37985"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9960362.1"
                     /translation="MDNLTPPSRSKAGAELCAPPRAAALVHLSYAQRRLVEAIGRRLG
                     VRDSELQDLVQRVVLTMFRKAELVQTGSERAFLSEVTRREARHILRSGRRHAALCSAQ
                     LAELGSAGHCPEEPSSRLDDSRRLQLALAEISAPLLEVWIRHVLDGASCQAVASELGL
                     PLGTVKSRLRRVWARVFEASNEGKSHGNQAQIRMHSMEPSAALRALGECSTSHDEAEG
                     SRFET"
     gene            complement(6145..>6421)
                     /locus_tag="VFQ61_37990"
     CDS             complement(6145..>6421)
                     /locus_tag="VFQ61_37990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960363.1"
                     /translation="PGTLAPNMQLIAKLASVSSLARQFTSDAEAEPEPVPIANFTPSA
                     DPLLIELPEFDPTAAALSYIVNVDGVDVKATESVILESAPSEEEVLF"
BASE COUNT         1178 a         1963 c         2126 g         1154 t
ORIGIN      
        1 gcctttggcg gaagcgccga cctagcaaac atcgctctga acgccgcttg caccgcagac
       61 catccccact gcggccaact tggactattc gcaaaaccgt agaacccccg ctctgaagaa
      121 gggcgcccgc tcgtgaatgc cgggcgggcg cgaccccgcg tcgtggtgcc caagaccgca
      181 gccgcgagcc tcctattgac ccgcaacacc gcgcggcccc cgcaacaacg acgttccccg
      241 gcgtcgagtt attgggggtg gcctgcgcgg tgccgcgacg acaggcgacg gatggagacg
      301 gcgctctcga gcgcccggac gcacgtcgcg cccgaaaaca gagtggccgc caaacggctc
      361 cgccgattca acgaattgac gactgcctag attgacagtc aacgttggct acgtagaccg
      421 gagaccaaaa ctgctactgc agcggaacct ctgcgaccaa gtcgcgcgcg aacgaacgtg
      481 tgccgcgtag ttcgggatcc aacaggaact ctcgccggta gatgaggagg gtttcggcgt
      541 cgccagtttc gccgacgctc tcacgcacga agcgcgcgat gaggtcgatg tagcgctgct
      601 tgtcctcctc gctcatcttc ttggggcggt acttggggtc gaagccaggc gcgacttcgc
      661 gggcaattcg ataggcttcg gcgttgtctt gcagatgggc aaaggtcaaa cgaccgagtt
      721 cgcgacgctg ctcgtcgggg ccgctcagcg cgaactcgag ccagctgcct cgctcggggg
      781 cctttccgac gcgcgagttg aacagcggat agaccgagat gggccaggaa tctaccggca
      841 cgagcccggc gtacaacatg ctggtgagta gcagaagccc gacgagtagc ggtggcgccg
      901 atgcggaata atgcgagcgg ttcgaatcga gggcaggggc gggttgcggg cgtaagctca
      961 atcttcgcct cgcagtctcg aatgccgcta tgagaatcgg ggcggcgtag cggaagggcc
     1021 agaggcgcag aaccgccggg aaatcgatga acacgatgag tggaaagaag accgagaacc
     1081 agattcccat ggtcaggccg accccggtgt ggaacagcgc ggctgccaga gcgccgagca
     1141 gccgcgtggg gcgccacaag attgcgaaga agaagccaat ctcgaagatc agcgtgaagg
     1201 ctccgccgat ggccagcagc cacttcgagt gatcgacgcg cagcgcgggt acgaactccg
     1261 gagactgagc ccactttttc gccatttcaa cctgcagctt ggtcccattc agccactggt
     1321 caccggtgtc ccatagcttc caaacgcccg gaaagaggta catcgtcccc aggatcagcc
     1381 agcaaagacg cacgggaatg gtgtatgcgg ggccgggtga gggcgcgggt tggccccgat
     1441 actggcgaat ccaaacatcg atagccaaac cgtcgccggc gcgactggag gccaacacca
     1501 aggccagcag taccggcatg tggagcccgt ggctcacctt gaagaagaag gcgggtacgc
     1561 cgaggaccag cacggcgagg atggccgcga gtttggtcgt gacgcgcgcg agcaccccca
     1621 agctcgcgag ggctgcgcac acgcacagca aggtttgcgc cggttttacg taggggagcc
     1681 agctctcgaa ccaggcgccc aaccactccc agccgcgtgg aggagaaacg aattctttgg
     1741 gcagcgttgc gtaccaccac gcctggctgt ggcacgcatc gtaaaagacc gtcccgaaca
     1801 ccacggcgcg cagcaacccg agattggcgg ggtgggtggg ctcgagcaga aactcgcgcg
     1861 ccttttgcca cgcttgctcc gggcgcaaca tgatgagtgc aatgccgagc gcgaaggtgg
     1921 ccaaaagggc tagccgcaaa gcgaatagcg tgttcatcct cttccccaag ggctcgtatg
     1981 tttggtgcgc ccacgccttt cagagcgttc gtaagggcgt gagagcgatg gccgtctcat
     2041 accgcatccg agacgcgatt tgaactcgtt ggggcgctgc tcgcgcttgt ggcctcgttc
     2101 gttggatgcc ggggtagtcg tggtcgattt gagcagtagc cactctggta ttccttcgcc
     2161 aaaggccgcc cgcgggcacc ccgccgttct cgtgctagac cacgcggcct gatcgttttg
     2221 ggaggtagta tggttttcga ttggtcccgc tatacgggta acattgcgtg gctcgctcaa
     2281 cggacgatct atctcgcccg ccatggatcg cacgcctacg gcaccaacat cgcgaccagt
     2341 gacgaggact ttcgtggagt cgcgatcgcg ccgcgcgagt tctatctggg cggtctacat
     2401 cgattcgatc aggcggagtg caagtcaccc gatttgacga tcttcgattt gaggaagttc
     2461 gtggggctcg ccgcgcaggc gaacccgaac gtgatcgaag tgctgttcgt ggagaatgcg
     2521 gatcgattgc aggttacggc cgcgggcgag cggctgctct cggcaagaga gttgttcgtg
     2581 acgcggcgga ttcgtcatac gttctcgggt tacgcggcct ctcagctgaa gcgcattcgc
     2641 gcccaccggc gctggttgat gaatccacca gcggctccgc ccgagcgcaa ggctttcggc
     2701 ttgccggata cgacggccat cccgggggac cagctgctcg ccgccgaggc cgcgattcgg
     2761 gctcagctgg acgcgtggag cgccgattac ttggatgagt tggacccggt gattcgcgag
     2821 acgatcacgg gcaagatgac gcgccatctg gccgagatgg gtgtggccat gaatgaggac
     2881 ctctggcccg gggcggcgcg cgtgatcggg ctcgatgaca acttcatcga gtatctggcc
     2941 cgcgagaaga agtacaacgc ggccaagaag gaatgggaga actaccaaac ttggcggcgc
     3001 gagcgaaacc cggcgcgggc ggtgctcgag gcaaagtttg ggatcgatac gaaacacgcc
     3061 atgcacctga ttcggctcct gcgcatggcg cgggagatct tggaaaccgg gcgagtcatc
     3121 gtgcgacggc cggatgcgga ggagttgctg tcgattcgca acggcgcctg gtcttacgag
     3181 cgcatcgtcg actgggcgga gcaagaagac gcagccttac aggaggtcgc ggcaaagagc
     3241 actctaccga aagcgcccga cgtcgaagcg attgaccgcc tgtgtgtcga actgattggc
     3301 gaagccatcg ggtgctaacc gtggatcgcg ggctttcggg atctgattgc gctcccgggt
     3361 tcagccgccc atgcagaaat gcatgggcgc aatgcacgga tcgagactgg tgagacgcgg
     3421 cgctctcgcc caatgctcgg ggcatgagat tgggtcaggt tctctcggtc gttctgtttg
     3481 cggcttgtga ggcacacgcg ccgtcggtcg gtagccgcga cgatccccgg gcggtgccgt
     3541 catcgacggt gccctcgtcg acgaccccag tggtcgagcc ggcgcttcgc gtccgttggc
     3601 tgggcgggcc cacggcgacg ttggagcgca gtggcttgcg cgtcttgacg gatccgatgt
     3661 tgggcccgcg cgaggacgtt gcattcaggc tgccgaaaca cccctcgagt ggagtgctgg
     3721 acgcgcccgt ggcgcgctac acgtctccgc ccgcgttcga tatgcagggt ctgaccgcga
     3781 ttctgatcag ccacactcac aacgatcact tcgatgcgac ggcgcaaagg actttgccga
     3841 agtcactgcc cgtggtggtg gcgaaagcag gggcagataa ggtgcgggcc gcaggttttg
     3901 cggatgtgag gccgctcgat tggagtgagt cgctcacgct cgagcacgcg ggtacgaacc
     3961 tgaagatcac ggccgtgccc gcgcaccacg cgcatgacgc cggattggac tccgaactcg
     4021 gtaggggcaa cggttacgtg ctggagtggc gggactcacg cggagtgtgc cgggtgtact
     4081 ggacggggga tgcggtgctc agcgatgatt cgcgcggctt ggccgagaag tacggcccga
     4141 ttgacttgct cttgcctcat ttggggggcg tgggcgggga cggcggattg gggctgcgca
     4201 ccatgaatgc ggcggagaca ctcgggttgg tcgagcgcgt caatccggct ctcgtgattc
     4261 ccattcacca cacgacgttt gctcactatc gcgagcccat cgaggcgttg gagcagcgcg
     4321 ccggcgaagc ggggctggcg aagagctttc actttttgcg ggagggcgag agcttcgacc
     4381 ttcaacgcag atgaccgatc cacgcgtcac gagggcgatg cggcgcgatc ggcccaggcg
     4441 ggtaccggcc ccgcccgggc cacgacaagc tcatttcatg gacaacagtt gatggtctca
     4501 cagccgatga aggtgtcggt gccgcagtcg ccgtagcaga cgtcggtgcc ggggccgcct
     4561 tgcacggtgt cggccgcgcc gtcgcacgca aacaggacgt cgtcgtcgtt gccgccgttc
     4621 agggtgtcgg cgccgtcgcc gccgtacaat acgtcgttgc cgtcgccgcc gcgtagggtg
     4681 tcgtccccgt tttcaccaaa cagggtatcc gcgccgttgc cgcccaagac ggtatcgtcg
     4741 ccgtcgccgc cgtggaggtt atcggcgtcg tcgccgccat ctacggtgtc ggcgccgttg
     4801 tccccgtaga ttaggtctgc gccgttgccg ccgtggagct cgtcggcgcc gtcatcgccg
     4861 tggagggtat ccacaccatc tccaccccac agggtgtcgc cttggttacc gccgttcatg
     4921 ttgtcgtcgc cgttgccacc ggtgagggtg tcgacgccgt caccaccatt caacgtgtca
     4981 tcaccgtcgc cgccattgag ggtgtcgttc ccgtccaggc ctgacaagat gtcgttgcag
     5041 tttccgccgt tcaggacgtt ggcgcccatc gtgccggata cgacgtcatt gtcggggccg
     5101 ccggcgaatg cggcctgccc cgcgaggaac acgcccgccg cgcaaaccaa gaggggctga
     5161 tcggtcttgg cgacggagct cgtggactcg gtgctctcca gttcgctgcc ggcgtattcg
     5221 gcgctgcagc cgccaagtgc gcacaccaga ccgaagccac tcaaaattat gccacccatt
     5281 cgaatcatca tgactgaact cctcattccc tagaactcac gtctcgaatc tcgaaccctc
     5341 agcctcgtca tgcgaggtcg agcactcacc caacgcccgg agcgcggccg agggttccat
     5401 cgagtgcatg cgaatttggg cttggttgcc atgcgacttg ccctcgttgg aagcttcgaa
     5461 gactcgtgcc cagacgcgcc gcagtcgtga tttgacggtg ccgaggggca accccagctc
     5521 actcgccacg gcttggcacg aggcgccgtc gagcacgtgt cgaatccaca cctccaacag
     5581 cggggcggag atctcggcga gggccaattg tagtcgacgg ctgtcgtcga gccgagaaga
     5641 aggctcttcg gggcagtggc ccgcgctgcc aagctcggcc agttgtgcgc tacatagggc
     5701 cgcgtgacgg cgcccactgc gcaatatatg tcgcgcttcc cggcgcgtga cctcggatag
     5761 aaacgcacgc tcggagcccg tctgcaccag ctcggctttg cggaacatcg tgaggaccac
     5821 gcgttgcacc agatcttgga gctcgctgtc tcggacgccg agtctcctgc cgatggcttc
     5881 caccaatcgc cgctgcgcat aagagaggtg caccagggct gcggcgcgcg gcggcgcgca
     5941 gagctccgcg cccgccttcg acctcgaagg aggtgtgagg ttatccatgt ttgctcctga
     6001 gagcgccgcc tcgccgcggc ttgagttcac ggcggatcgg tcgagttgta atacctatgg
     6061 gttattagcg tagacgcgtg tttccggtcg agcgaactcg gcggggttca cgctcagctt
     6121 gcagtggaaa gcggagagag cctgctagaa gagcacttct tcctcgctcg gagcgctctc
     6181 caggatcacg ctctcggtcg cctttacgtc caccccatcc acattcacga tgtacgagag
     6241 cgccgccgcc gtcggatcga attcgggaag ctcgatcagc agcggatcgg cagagggcgt
     6301 gaagttggcg atgggcacgg gttccggctc ggcctccgca tcggacgtga attgtctggc
     6361 cagtgaagac acgctcgcca gcttggcgat cagctgcata ttgggcgcca aagtacctgg
     6421 t
//
LOCUS       DASTKU010001069         3297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1774781, whole genome shotgun sequence.
ACCESSION   DASTKU010001069 DASTKU010000000
VERSION     DASTKU010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774781"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFQ61_37995"
     CDS             complement(<1..321)
                     /locus_tag="VFQ61_37995"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HET9960364.1"
                     /translation="MQIRSLSLIDGRERSATGPRLRVAVATQDLKSVNAHFGSARKFA
                     VYEVTPDGSRFVQAFDFESTSDESGEHKTEVDKISPKVSALAGCNLLFCLAIGGPVAA
                     KVISA"
     gene            complement(308..1699)
                     /gene="nifN"
                     /locus_tag="VFQ61_38000"
     CDS             complement(308..1699)
                     /gene="nifN"
                     /locus_tag="VFQ61_38000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifN"
                     /protein_id="HET9960365.1"
                     /translation="MAILTASKKACAENPLKSSTPLGAALAFLGIEGSVPLFHGSQGC
                     TSFALVLAVRHFKEAIPLQTTAMNEASTVLGGSDHFEEALVNITTRMKPKFIGVASTA
                     LVETRGEDFAGDLRNIVAKRRELDPVRVVFAATPDFEGALEDGWAKATSAIIEALVPA
                     QELPRAEPDRAERIINILPGVHETPASIEALLQLAAAFGLRGRVVPDISGSLDGHLPD
                     AYVGTSLGGAPLAEVEQLGTALHTLALGEHMRAPAELLRQRTGVAATVLPTWIGLHAS
                     DQLVHWLSVISGQPAPAWVRRQRSQLVDAMLDGHFHFSGKRIALASDPDLLHGLAHFF
                     SALGAQVVIAISSTQNSPLLTDLPCEEVLVGDLGDLEERAAAAGVDLLVTHSHGRQAS
                     EKLGIPLFRVGFPIFDRLGHQHRCLGGYAGTRELIYEIANLFLARLHEHSPEDFGSAV
                     PPLNVMDSRHADP"
     gene            complement(1737..3188)
                     /gene="nifE"
                     /locus_tag="VFQ61_38005"
     CDS             complement(1737..3188)
                     /gene="nifE"
                     /locus_tag="VFQ61_38005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifE"
                     /protein_id="HET9960366.1"
                     /translation="MSDALKAKIADVFNEPGCEKNQQKGAKERKKGCSKPLTPGAAAG
                     GCAFDGAKIALQPITDVVHLVHGPLACEGNGWDNRHAATSGSSLYRRGFTTDMSELDI
                     VMGNGEKRLYKAIKEAVQKHSPPAVFVYSTCVTALIGDDIEAVCRHASEKLNTPVIPV
                     NAPGFVGAKNLGNKLAGEALLDHVIGTREPETITPMDVNILGEYNLSGELWQVKPLLE
                     RLGVRLQACITGDARYHEVAASHRVSVNMMVCSTALINLARKMEERYGIPYFECSFYG
                     IEDTSAALRTLAKLLVERGAPGDLLERTEALIASEEARAWARIKPYQKRLEGKRVLLY
                     TGGVKSWSVVSALQEIGMIVVGTSVRKSTKEDKERIKELMGGDAHMVDSIPPRQMYEQ
                     LARGEADVLLSGGRTQFVALKARVPWLDINQERHYAYAGYDGMVELVRQLDLEINNPV
                     WSEVRRAAPWEPSYADGLGGAADDGATALAVSA"
BASE COUNT          603 a         1078 c         1000 g          616 t
ORIGIN      
        1 cgcagaaatg accttggcgg ctacgggccc accaatggcc aaacagaaca acagattgca
       61 acccgcgagc gcgctcacct tgggcgagat cttatcgacc tctgtcttgt gttcgccgct
      121 ctcgtccgag gtgctctcga aatcgaaagc ctgtacgaat cggctgccgt caggcgtgac
      181 ttcgtagacc gcgaatttgc gtgcagagcc gaagtgcgca ttgacgctct tcagatcctg
      241 cgtcgcaacc gcgacgcgga ggcgcgggcc agtggcgctc cgctcgcgac catcgatcaa
      301 agataggcta cggatctgca tgacgactgt ccatcacgtt gagggggggg acggcgctcc
      361 cgaagtcttc cggcgaatgc tcgtgcaagc gagccaagaa gagattcgca atctcgtata
      421 tgagctcgcg ggttccggcg tatccaccca ggcaacggtg ttggtgcccg aggcggtcga
      481 agatcggaaa gccgacccga aacagcggaa tgccgagctt ctccgaagct tgtcgcccgt
      541 ggctgtgcgt caccagcaaa tcgaccccgg cggcggcggc ccgctcttcc aagtccccca
      601 gatctccgac caacacctct tcgcaaggaa ggtcggtcag cagggggcta ttctgagtcg
      661 agctgatggc aatgacgacc tgcgctccga gcgcgctgaa gaagtgagcc agcccgtgca
      721 ggaggtccgg gtcactcgcc agcgcgattc gttttccgct gaaatggaag tggccgtcca
      781 acatcgcatc gacgagctga ctgcgctgcc ggcgcaccca agccggcgcg ggctgcccag
      841 aaatcaccga tagccaatgg accagctggt cacttgcgtg gagtccaatc caagtgggca
      901 gcaccgtggc tgctactccc gttcgttgac gcagcaactc ggccggcgct cgcatgtgtt
      961 cgccgagagc caaagtgtga agggcggtgc cgagctgctc cacttcggcg agcggggcgc
     1021 cacccaagct ggtcccgaca taggcgtctg ggaggtgccc atcgagcgat ccggaaatgt
     1081 ccggcactac ccgcccgcgt agcccaaacg ccgccgccag ctgtagcaga gcctcgatgg
     1141 aagcgggcgt ctcgtgcacg cccggtaaga tgttgataat gcgttcggcc cggtccggct
     1201 ccgcccgggg caactcttgc gcgggcacca aagcctcgat gatcgcactg gtcgctttcg
     1261 cccagccgtc ctcgagcgca ccctcgaagt ccggcgtcgc cgcgaacacc acccgcactg
     1321 gatcgagctc acggcgcttg gcgacgatgt tgcgcagatc cccggcaaag tcctcaccgc
     1381 gcgtctccac cagcgccgtg gacgcgacgc cgatgaattt gggcttcatg cgcgtcgtga
     1441 tgttgacgag cgcctcctcg aagtgatcgc ttcccccaag caccgtcgag gcttcgttca
     1501 tggccgtggt ctggagcgga atggcctctt tgaagtggcg aacggccagt accaacgcga
     1561 aggacgtgca gccctgcgaa ccgtgaaaga gcgggacgct accctcgatg cccagaaacg
     1621 ccaacgccgc ccccagcgga gtggaagatt tgagcgggtt ctccgcacag gcctttttgc
     1681 tcgcggtcag gattgccata tcgaggctcc caatcgtttc gtgttcggcg gttggctcac
     1741 gcggagaccg ccagcgcggt cgcgccgtca tcggcagcac cacccagtcc gtctgcatag
     1801 ctcggctccc acggtgccgc gcgccggacc tcgctccaaa ccgggttgtt gatttcgagg
     1861 tcgagttggc gaacgagctc gaccatgccg tcgtagcccg cataggcgta gtgacgctcc
     1921 tggttgatat cgagccaggg aacgcgggcc ttcaaggcca cgaactgggt gcgtccgcca
     1981 gacaacaaca cgtcggcttc gccgcgcgcg agctgctcgt acatttgccg aggtggaatc
     2041 gagtccacca tgtgcgcatc cccgcccatt agctccttga tacgctcctt gtcttctttg
     2101 gtggacttac gcaccgaggt accgaccacg atcatgccga tctcttgaag cgccgacacc
     2161 accgaccagg atttgacccc gccggtgtac aacagcacgc gcttgccctc gagtctcttc
     2221 tggtaaggtt tgatgcgggc ccaagcgcgg gcctcctcgc tcgcgatcag cgcctcggtg
     2281 cgttcgagca ggtcacccgg ggcccctcgc tcgacgagca gctttgcgag cgtgcggagc
     2341 gcggccgacg tgtcctcgat tccatagaac gaacactcga agtaagggat tccgtagcgc
     2401 tcctccatct tgcgcgccaa gttgatgagc gcggtagagc acaccatcat attgacgctt
     2461 acgcgatgtg aagccgccac ctcgtgataa cgtgcgtcgc ccgtgatgca ggcttggagc
     2521 cgaaccccca agcgctcgag caacggcttt acctgccaca gctcacccga gaggttgtac
     2581 tcacccagga tgttcacatc catcggggtg atcgtctccg gctcgcgggt accaatcacg
     2641 tgatcgagca acgcctctcc cgccagcttg ttgcccaagt tcttggcacc gacgaagccc
     2701 ggggcattca ccggaatcac cggggtgttg agtttctcac tcgcgtgtcg gcagaccgcc
     2761 tcgatgtcgt cgccaatcaa cgccgtgacg caggtggaat agacgaacac cgcgggggga
     2821 gagtgctttt gaactgcttc cttgatggcc ttgtagagcc gcttttcccc gttgcccatc
     2881 acgatgtcca gctcgctcat gtcggtggtg aagccgcgcc ggtacaagga ggagccggac
     2941 gtggcggcat gccggttatc ccaaccgttg ccctcgcacg ccagcggccc gtgcaccaag
     3001 tgcacgacat ccgtgatcgg ttgcaatgcg atcttcgcgc cgtcgaacgc gcagccgccc
     3061 gccgccgctc caggggtcaa gggcttcgaa cagcccttct tgcgttcctt cgcccccttc
     3121 tgttggttct tctcgcaacc cggctcattg aagacatcgg cgatcttggc ttttagggcg
     3181 tcagacatgg ggaccgctct ccgacatcgc actgagagcc tattgggatg gctcttagga
     3241 ggtggggccc cagcgacgga gtcgatcttc ggagtggatc gactccgcgc tggggcc
//
LOCUS       DASTKU010001070         3367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_433876, whole genome shotgun sequence.
ACCESSION   DASTKU010001070 DASTKU010000000
VERSION     DASTKU010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433876"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="VFQ61_38010"
     CDS             complement(<1..1293)
                     /locus_tag="VFQ61_38010"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET9960367.1"
                     /translation="MDPLPRPNAAGLILACLPAVFAASFAAASAAMAAFSPARRAALR
                     DILDGGSKAALERYLESGVSIESRWLVVRALGMSLSAILWVEQLPMHWGALRLVGATF
                     CVVLAYAVPSEIGRALVSRDPERAGPLLLKILYPIELLAAPISAPILWLSQSIGGWLS
                     SPPSQTPGVTETEVEMIVTEGELNGSLAHEQSEMIRNVLEFGDVTAGQVMVPRTQVTA
                     LSIDTPLDEALQRIAESGHSRYPVYRGSVDNVVGLLYAKDLLRHAADSDSAEQTLEGL
                     VRTPVAFVPDSQSASSVLKDMRAGRHHLAVVIDEFGGMNGILTLEDLLEKIVGDIRDE
                     HDVEEPPIVDLGNGRLVADASVPIADLSRYLGANLPSDGDYHSLGGFMIAQLGRVPEV
                     GARLAAFGLEFEVREGDERRVSKIEITRLSPPESVVPRS"
     gene            complement(1640..2578)
                     /gene="truB"
                     /locus_tag="VFQ61_38015"
     CDS             complement(1640..2578)
                     /gene="truB"
                     /locus_tag="VFQ61_38015"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET9960368.1"
                     /translation="MKGQQVDGVLPVDKPQGKTSHDVVAEARRLFGTRAVGHAGTLDP
                     MATGVLLLLFGEACKLSNYLTAEYKTYRARVRFGASTDSLDADGRVLESVELAPSWLS
                     EEALESALRVERCRLEQVPPAVSAIHSDGERAHVRARRGEAVELPPRPVAVRELRLLR
                     REAHELELELCVSKGYYVRSLARDLGSSLGQPAHLSALRRLSSGAFTLAHSVAWPCVE
                     RPSLMPTVEAACRALPVLRLTESGVLFARQGKRLGPEQVKTPAAPAELPPIQAKGTEL
                     EREPQVSVWLSPAGELVALGTWDGERHRVVRGFASN"
     gene            complement(2590..>3367)
                     /locus_tag="VFQ61_38020"
     CDS             complement(2590..>3367)
                     /locus_tag="VFQ61_38020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9960369.1"
                     /translation="EEVDDFGLDPGFESKLRGVLDMLYRRYFRVEVANIERVPAEGRA
                     VIVANHSGTLPLDGAMLRTALRLDHPKARDLRWLAEDFVFHLPFLGVFLNRIGAVRAC
                     QENAERLLEKDSVVAVFPEGVHGIKKLFNQRYQLQRFGRGGYIRLCLRMRAPLVPCAI
                     IGAEETNPLIFRFERVAEMFRLPYLPVTPTFPWLGPVGLLPAPTRWKIRFGEPISFDA
                     YGPEAADDNVLVGKLSERVRSSIQSLIDVGLRERKSVWFG"
BASE COUNT          642 a         1091 c         1060 g          574 t
ORIGIN      
        1 cgagcgtgga accaccgact cgggcggcga gagccgggta atctcgatct tggagactcg
       61 acgctcatcg ccctctcgaa cctcgaactc gagcccaaac gcggcgagcc tcgcccccac
      121 ttcggggacg cgcccgagct gcgcgatcat gaagcccccg agcgagtgat agtccccatc
      181 ggacggcaaa ttggcaccca agtagcggct caaatccgcg atgggcacgc tcgcatcggc
      241 gaccaatcgt ccgttgccga ggtcgacgat cgggggctct tcgacgtcgt gctcgtcgcg
      301 aatatccccc acgatcttct cgagtaggtc ctcgagcgtg agaatcccgt tcatgccgcc
      361 aaactcatcg atcacgactg cgaggtggtg gcgcccggcg cgcatgtctt tgagtacgct
      421 gctcgcggac tggctatcgg gaacgaatgc caccggtgtg cgcaccaggc cctcgagcgt
      481 ctgctctgca ctgtccgagt ccgccgcgtg cctcaagagg tctttggcat agagcaggcc
      541 cacgacgtta tcgacgctgc ctcgatatac ggggtaacgc gagtgaccgc tctccgcgat
      601 tctttgcagt gcctcatcca ggggggtatc aatcgagagc gcggtcacct gtgtgcgagg
      661 caccatcacc tgaccggcgg tgacatctcc gaactccagc acgttccgaa tcatctccga
      721 ttgctcgtgc gccagggagc cgttgagctc accttccgtc acgatcatct ccacctcggt
      781 ttcggttaca ccgggcgtct gcgatggggg cgaggagagc cagccgccga tgctctgact
      841 gagccataag attggcgccg agattggggc tgccaagagc tcgatcggat acaagatctt
      901 caacagcaac ggccccgccc gttcaggatc ccgagagact agggctcgtc cgatctcgct
      961 tggcacggcg taggccagca ccacgcagaa cgtcgcaccc accagtcgca acgcgcccca
     1021 atgcatcggc agctgttcga cccataaaat cgcgctcaag ctcatgccca acgcgcgcac
     1081 gacgagccac cgcgattcga tggagacgcc cgactccaaa taacgttcga gagccgcctt
     1141 cgacccgccg tcgaggatgt cacgcaatgc cgcgcggcgc gcgggagaga aagcagccat
     1201 ggcggcgctg gctgcggcga acgaggctgc gaagaccgcc ggcaaacaag ctaaaatcag
     1261 gccggcggcg ttgggtcttg ggagcggatc cacgaagttt ccttgcagtg agttttgggc
     1321 acacagggcc cagctgacag tccaaaaagg gctggctcgc ccagagccta gcggagtgct
     1381 ggacggccac gcaaccgagc ggcggttcgg ctcgtgtagg tcgaagctga cacgtagggc
     1441 gggatcaatc cgcgggccga cagggcaaaa gtacctgcca tctccacaaa agcctctgat
     1501 tttcgggttc ttagcgacgc tcaggaggga gcgaagcaca cgtcgatgaa gtcgtcatcg
     1561 gctttatggc cgacactcgc ataccccaca agttttgtaa gacagccgcg gagctgattc
     1621 gcttcaatcg tggtccgctt cagttggacg cgaagccgcg gacgacgcga tggcgttcgc
     1681 catcccacgt cccgagcgca acgagctccc ccgccgggga gagccacacg ctcacttggg
     1741 gctcccgctc cagctcggtg ccttttgcct gaatgggagg gagctccgcg ggagctgccg
     1801 gggtttttac ttgctcgggg ccgaggcgct tgccttggcg tgcgaacaga accccgcttt
     1861 cagtcaagcg caagaccggt agcgctcggc atgcggcttc gacggtcggc atcagcgatg
     1921 ggcgctcgac gcagggccac gccacactgt gagcaagggt gaaggctccg ctgctgaggc
     1981 gacgcagcgc gctcaggtgc gcgggttggc cgaggctcga tcccaggtct cgggccaacg
     2041 agcgaacgta gtagcccttg gaaacgcaca gctcgagttc gagctcatgt gcctcacgac
     2101 gcagcagccg tagctcacgt acggcgaccg gtcggggcgg gagttccacc gcttccccgc
     2161 gacgcgcacg gacgtgggcg cgttcaccgt cactgtgaat ggcactcact gcgggcggca
     2221 cctgctcgag gcggcatcgc tccactcgca gggcgctctc caacgcctcc tcactcaacc
     2281 aactcggtgc cagctcgacc gactcgagga cccgtccatc ggcatccaga gagtccgtgc
     2341 tggctccgaa gcggacgcgc gcgcgatagg tcttgtattc cgcggtcaga taattggaga
     2401 gcttgcaggc ttcaccgaag agcaacagca agaccccggt cgccattggg tccaacgtgc
     2461 cggcgtggcc gaccgcgcgc gtgccgaaca agcggcgtgc ctcggcgacc acgtcgtggc
     2521 tggtcttgcc ctgcggttta tcgacgggga gcacaccatc gacctgctgg cccttcatgg
     2581 cgaggtttcc tatccgaacc agacgctctt tcgctcgcgc aacccgacgt cgatcaaaga
     2641 ctgtatgctg ctgcgaacac gctcggacag cttgccgacg agtacgttgt catcggcggc
     2701 ctccggacca taggcgtcaa aggagatagg ctccccgaat cggattttcc agcgcgttgg
     2761 tgcggggagt agaccgaccg ggccgagcca cgggaaggtg ggcgtcaccg gcaagtacgg
     2821 caggcggaac atctctgcga ctcgttcgaa gcggaagatg agcgggttcg tctcttccgc
     2881 gccgatgatg gcgcaaggca ccagcggggc ccgcatgcgc aagcacaacc gaatgtagcc
     2941 gccgcgcccg aatcgctgca gctggtagcg ctgattgaac agcttcttga tgccgtgcac
     3001 accttcgggg aatacggcga ctaccgagtc tttctcgagg aggcgctccg cattttcctg
     3061 gcacgctcgc acggcgccga tgcggttcag gaatactccg aggaagggca aatggaagac
     3121 gaagtcttcc gcgagccacc ggaggtcccg cgccttggga tgatcgagcc gcagcgccgt
     3181 gcgcagcatc gcgccgtcga gcggcagcgt gccagagtgg ttcgctacaa tcaccgcgcg
     3241 gccctcggcc ggcacgcgct cgatgttggc tacttcgacg cggaaatagc gtcgatacag
     3301 catgtcgagc acgccgcgca gcttggactc gaaccccgga tcgagaccga agtcgtcgac
     3361 ttcttcg
//
LOCUS       DASTKU010001071         4972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1105431, whole genome shotgun sequence.
ACCESSION   DASTKU010001071 DASTKU010000000
VERSION     DASTKU010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105431"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VFQ61_38025"
     CDS             complement(<1..920)
                     /locus_tag="VFQ61_38025"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HET9960370.1"
                     /translation="MSKTYRGAIRLGLWAGLTLTALSAFPAFAAAQAASRPDANRPPL
                     AQSLTGEALTNFESALLLYRDNDFAGAQLKFRRAFELSHDPRLLWNMAACEKNQRHYA
                     RVEQLLRQYVAAPQTTEGERGEAKVLLDTIGDFIGSVAIDVDQPGADVFVDDVHVGQS
                     PLPAPVRLDMGTRAVRVVKSGFRGYAAPVELTGGATTHISVRLAAEPREGKLRVSAAP
                     GATIRVDGVPVGNGTWEGNLATGPHQIEVTSEGIKPWRSEAVIQTGQLTSVLVSQANN
                     AAPAPSAGMKVIPAWAWVAGSAVLATGLGVG"
     gene            complement(917..2392)
                     /locus_tag="VFQ61_38030"
     CDS             complement(917..2392)
                     /locus_tag="VFQ61_38030"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9960371.1"
                     /translation="MIHGFSQIPVGAVIAGKYRVERTLGTGGMGYVLAATHLELQQRV
                     AIKLLHGSASHSTEAVARFVREARAASRIQSEHVARVIDVGRLENGERYTVMEFLEGR
                     DLSVLLQQGPLPVQTAVDYVLQACHAVAEAHAAGIVHRDLKPSNLFLSRRPDGSGIIK
                     VLDFGISKLTTGDNFDAHLTSTQTAMGTPLYMSPEQMRSPRDVDLRTDLWSLGAILYE
                     FLSGRPPFMANSFPELVSAILESTPVPLSSANAQIPAALEAAVARCLEKGVARRFQSV
                     GEFARELAPFASRESQFLVERVARLSGAEFISVPPEVRSDSHPKGVVSARPVPTHANW
                     AHTQSAKPQRRMRVLIAAAGIGVLAAGAFAYRSWESTRANLGVVNGIDSARLTRASAA
                     AVTTTVSEPAAPEGVTVAPVNTPEPLPRATSVSSSPPLRPSALASPGASQVGSPQLSP
                     AVLGIPKNKASTQPAAAAASAAPAPNSSGSASKTLNMDLKQ"
     gene            complement(2398..4596)
                     /locus_tag="VFQ61_38035"
     CDS             complement(2398..4596)
                     /locus_tag="VFQ61_38035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HET9960372.1"
                     /translation="MTAQVTKRRTGSRWFRGLLGLAAVLGGSCSDSNAPPRGQLMIAL
                     ATDMSIPKDVTTIRVKVKLNAEVRYEYDFAIAPDGEFHIPGTVAVVEGSTPNPVVAVE
                     VVGIRTRKSGAVEARTFSKSVTTIPRERIATLRIPVQWLCDGTAVDDGTGEYLSTCAP
                     ENGEETACRAGECKPVAVPPETLETFDPKALYGGAATPEDPLARCFDTVACFEKGADL
                     EVPDGQDCVLSVAGEPSQLNVALRMPASGDGICPEGNNEGGCFVPLDQDRDWGWSFEP
                     GAEAGRTTLRLPRAACEAVRKGRALGLRVSSACESKTSKYPTCGPWSAVERAFTSQPQ
                     GGSGGSGGSSASTQGGTLGTETTANRGGTTGTLASSGSAGEPTTSGGAAGGAAEGGAG
                     GDTSSVQGGEAGQAGGTDVGPTSCVGDIVFPDAAVEAIVRTRLEKPEGPISALEVASL
                     TSLELNQVSDLKGLECFTSLTSLRISGPEDVPDVRPGVSDLSPLARLRGLTSLIIETQ
                     NVADLGPLRDLNRLATLSLRRNHIDDVTPLANLTELDELDISQNRLSDVRALAALTKL
                     GALRTDAVGDLSFLRELPALRKLWLESGTAPIAPAVQALTQLTELSLNDLDLTGVSAL
                     SDLVNLETLVLKNNPIRSLTGLSNLTRLRILDLSYTLVIDLSELTNLINLRELNLEGT
                     EANDFSALIQNEGFGQGASLILNRSDCSEQIWALQERGVVVSYQENCESF"
BASE COUNT          870 a         1595 c         1582 g          925 t
ORIGIN      
        1 cccaccccta gccccgttgc taacaccgcg ctccccgcta cccaggccca agccggaatg
       61 accttcatgc cggctgaagg cgccggcgcc gcattgttag cctgggagac gagtacactc
      121 gtgagttgac cagtttgaat gaccgcctcc gagcgccacg gtttgatgcc ttccgaggtg
      181 acctcgattt gatgagggcc ggtcgccaaa ttcccctccc aggtgccgtt gccgacgggg
      241 acgccgtcga cacgaatggt ggcgccaggg gcggccgaga ctcggagttt gccttcgcgt
      301 ggctcggccg cgagacgcac cgagatgtgg gtcgtcgcgc cgcccgtcag ctcgaccggc
      361 gccgcgtagc ctcgaaagcc tgacttgacg acgcgaacag ctcgggtacc catgtccaga
      421 cgtaccggag ccggaagtgg agattggccc acgtggacgt catcgacgaa aacgtcggcg
      481 cccggttggt cgacgtcgat tgcgacgcta ccgatgaagt ctccaatcgt gtcgagcagc
      541 accttggcct ctcctcgttc accctcggtg gtttgcggcg cggctacgta ttgccgtaag
      601 agctgctcga cgcgagcgta gtggcgttgg ttcttctcac aagccgccat gttccagagc
      661 agccgaggat cgtgggacaa ctcgaaggct cttcgaaact tgagttgcgc acctgcaaag
      721 tcgttgtctc tatacagcag caacgcagac tcgaagttcg tgagcgcctc tccagtcaag
      781 gactgagcca gcgggggccg attcgcgtcc ggcctgctcg ccgcttgcgc cgcggcgaag
      841 gcagggaatg ctgacagcgc cgtgagggtc aaccccgccc aaaggcccaa gcggattgcg
      901 ccgcggtaag tcttgctcat tgtttcaaat ccatattcaa agtcttggat gcgctaccgc
      961 tcgagttggg ggcaggcgct gcagacgctg ccgcagctgc aggttgcgta cttgctttgt
     1021 ttttgggtat gccaagcact gccggcgaga gctgcggtga cccgacctga gaggcccctg
     1081 gggacgcgag agcggacggg cgcaggggcg gagaactgct gaccgatgtg gctcgaggca
     1141 aaggctctgg ggtgtttacc ggagccaccg ttacgccttc gggcgcggca ggctcggaga
     1201 cggtggtcgt taccgccgcc gcgctcgcgc gcgtaagccg tgcggagtcg atgccgttca
     1261 cgacacctag gttcgcgcga gtgctctccc aagaacgata ggcgaacgcg cccgcggcca
     1321 ggacgccaat ccccgcggct gcaatcaaga cccgcatgcg acgctgcggc ttagccgatt
     1381 gtgtatgcgc ccagttggcg tgcgtaggca cgggccgcgc gctcaccact cccttgggat
     1441 gcgaatccga gcgaacctcg ggcggaacac tgatgaactc ggcaccggaa aggcgggcca
     1501 cgcgctcgac gaggaattgt gactctcgtg aagcgaaagg ggcaagctca cgcgcgaact
     1561 cccccacact ctggaagcgc cgcgctacgc ctttctccaa acaacgcgca accgcggcct
     1621 ccaaggccgc ggggatctgc gcgttcgcgc tcgagagtgg gacgggcgtg ctctcgagga
     1681 tggccgaaac cagctccggg aaggagttcg ccatgaacgg cggcctcccc gacagaaact
     1741 cgtacaggat ggcgccaagc gaccacagat cggtccgcag atccacgtcg cgcggtgagc
     1801 gcatttgctc cggcgacatg tacaagggtg tgcccatcgc ggtttgagtg gacgtgaggt
     1861 gggcgtcgaa gttgtcgccc gtcgtcagct tcgaaatgcc gaaatcgagc accttgatga
     1921 tgccagagcc atcgggccgc cgcgaaagga atagattcga cggcttcaaa tcgcgatgca
     1981 cgatgccagc ggcatgcgcc tcggccaccg catgacaggc ttgcagcacg tagtccaccg
     2041 ccgtctgaac tggcagcgga ccttgctgga ggagaacgga taaatcccgt ccttccagga
     2101 actccatcac cgtatatcgc tcgccgttct ccaggcgccc gacatcgatc acgcgcgcga
     2161 catgctcact ttggatgcgc gacgccgcgc gcgcctcccg cacgaaccga gccaccgctt
     2221 cggtgctgtg agacgcactg ccgtgcagca gtttgatggc cacccgctgc tgcagctcga
     2281 ggtgtgtggc ggcgagcacg tatcccatac cgccggtccc gagcgtgcgc tcgacgcggt
     2341 atttgccggc gatcacagcg ccgaccggga tctgtgagaa gccgtggatc atcttgccta
     2401 aaagctctcg cagttctctt ggtagctgac caccacgccc cgctcctgaa gggcccaaat
     2461 ctgctcgcta cagtcggagc gattgaggat caggctcgcg ccttgcccaa agccttcgtt
     2521 ttggatcaat gccgagaagt cgtttgcttc ggtgccctcc aggttgagtt cgcgcaagtt
     2581 gattaagttg gtcaactcgg acaagtcgat gacgagggtg taactcagat ccagaatccg
     2641 cagtcgagtc aggttggaaa ggcccgtgag gctcctgatc ggattgttct tgagtacaag
     2701 cgtctcgagg ttgaccaagt ccgagagcgc cgaaacgccg gtgagatcca ggtcattcag
     2761 ggatagctcg gtgagctgag tgagcgcctg gaccgcgggt gcgatgggcg ccgttcctga
     2821 ctcgagccac agctttcgca acgccggtag ctcgcgcaag aagctcaggt caccgaccgc
     2881 atcggtgcga agcgcaccga gcttggtcaa tgccgcgagt gctcgcacgt ccgagaggcg
     2941 attctgcgaa atatcgagtt cgtcgagctc ggtcagattc gcgagcggtg tgacgtcgtc
     3001 gatgtggttg cgccgcaacg acagcgttgc caggcggttc aaatcacgta gcgggccgag
     3061 atccgcgacg ttttgcgttt cgatgatcaa agacgtgagc ccgcggagac gcgcgagtgg
     3121 gctgagatcg gacacgccgg gacgaacgtc cggcacgtcc tcgggcccgc tgattcttag
     3181 gctcgtgagc gaggtgaagc actcgaggcc cttgagatcc gagacctgat tcagttcgag
     3241 ggaggtaagg ctcgcgactt ccagtgcgct gatcggcccc tctggctttt cgagacgcgt
     3301 gcgaacgata gcttcgaccg cggcatccgg aaacacgatg tctcccacgc aggaggttgg
     3361 cccaacgtcg gtgccaccgg cttggccggc ctcaccgcct tgtacgctcg acgtgtctcc
     3421 gcccgcgccc ccttccgcag cgccaccagc agctccgccg ctcgtagtgg gctctcctgc
     3481 cgaaccggag ctcgccagcg tacccgtcgt tccgccacga ttcgcggtgg tttccgttcc
     3541 tagtgtgccg ccttgcgtgc tggcggagga tccacccgaa cctccgctgc caccctgagg
     3601 ttgactcgta aaggcccgct cgaccgccga ccacgggcca caggttggat acttgctcgt
     3661 cttgctttcg cacgccgatg agacgcgaag cccgagtgcc cgtcctttac gaacggcctc
     3721 gcacgccgct cgcgggaggc gcaacgttgt gcgccctgct tcggcccccg gctcgaagct
     3781 ccagccccaa tcccggtctt gatcgagcgg cacgaagcac cccccctcat tgtttccctc
     3841 gggacagatc ccgtcgccgc tggcgggcat ccgcaaggct acgttgagct gggacggctc
     3901 acccgcgacg gatagaacgc agtcttgccc gtcggggact tcgagatccg ctcccttctc
     3961 gaaacaggcc acggtatcga agcaacgggc tagcgggtcc tcgggtgtgg ctgcgccgcc
     4021 gtacaaggcc ttggggtcga aggtctctag agtctcgggc ggcactgcga cgggcttgca
     4081 ctcccctgcc cgacacgcgg tttcctcgcc gttttctggc gcgcacgtgc tcaggtactc
     4141 tcccgttcca tcgtcgaccg cggttccgtc gcacaaccac tggaccggga ttcgcagcgt
     4201 ggcgatgcgt tcgcgcggga tggtcgtcac ggacttgctg aaagttcggg cctccaccgc
     4261 gccggacttc cgcgtgcgga tccccacgac ttccacggca accacggggt tgggagtgga
     4321 accctcgacg acggccaccg tgccggggat atgaaactca ccgtcgggcg cgatcgcgaa
     4381 gtcgtactcg taacgaacct cggcgttcaa ctttactttc acgcggatgg tcgtgacatc
     4441 cttgggaatg ctcatgtcgg tcgccagagc gatcatgagt tgcccgcggg gcggtgcatt
     4501 gctgtcggag cagctgccac ccaagaccgc ggcaagcccc aatagtccac gaaaccacct
     4561 cgaacctgtc ctgcgtttgg tgacctgcgc tgtcatggcg tccggtttcg cttcgcttcc
     4621 gattttgctt ggcgatccaa accttcatgc ttacaggaag tctccgacgc tcgcaatctg
     4681 aacgcatttc gaggccgttg gcgcggtgtt ggcccgcctg ctgatttctt cggtgggctg
     4741 attcgagagg ttatcggctc gggtttcggc gctggccgtg gccgacgctc attcggagcg
     4801 gaccggacta gtgtcgatcg agcgcgccgc ccatgtgctt catcagcccg tcgagttcag
     4861 caagcagctt gcggtcggcc gcacgcggag gacgccgacc acgcggagtg ctcggcgcgc
     4921 tgggctgcgg atggggagtg gcgcgcggcg gctcccccgg accagcgtag tc
//
LOCUS       DASTKU010001072         9837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_434327, whole genome shotgun sequence.
ACCESSION   DASTKU010001072 DASTKU010000000
VERSION     DASTKU010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9837
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434327"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1482)
                     /locus_tag="VFQ61_38040"
     CDS             complement(160..1482)
                     /locus_tag="VFQ61_38040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HET9960373.1"
                     /translation="MSEESSLEKPTPPDGPERTASHEESALEAADQGPRVRILISAVL
                     VQLALGSVYAWSVFNKPLQTQFGWTKPQTVLPFETAIGCIVIGALLGGRAQDRHGPRP
                     VALIGGFLYAAGIMLASCVHRADQLWLLTLGYGVIGGTGLGAVYITPIAMLAKWFPER
                     RGLVTGLAVGGFGFGSVLAAPLARSLLANAGNKPSVFLPLGLAYLVLITLGALSFRNP
                     SSNKGGAGPKGGPQAPQLGQRELTLSQALRTPAWYQLTAILLLNVTAGIGLISQASDA
                     AQELGQLGPSAAAALVGIFGLFNGAGRVFYAGLSDRLGRMSVCSWLLFSQAVALALLP
                     LAHGAVLFFILAALVYTSYGGGFGVMPAASADFFGTKHAGSIYGAMIVAWSLGGVVGP
                     LLAAALYEVSGSYRVPFWSLAALALVSSLLPRWTRRPQLPPQPAETKV"
     gene            complement(2049..2534)
                     /locus_tag="VFQ61_38045"
     CDS             complement(2049..2534)
                     /locus_tag="VFQ61_38045"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET9960374.1"
                     /translation="MTNIAVQKDPNAPASQTPSTYREWDPFRALREIARWDPFARMAP
                     LWSGFSESGFTPAVEIKETPEEFVFTADLPGVKPDDLEVSLNQNRLTISGKRESERNE
                     KTDTYYTYERSYGHFSRAFNLPHETDANAVRAELKSGVLMVRVPKKAVSPPQKVPVTS
                     K"
     gene            2894..4069
                     /locus_tag="VFQ61_38050"
     CDS             2894..4069
                     /locus_tag="VFQ61_38050"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET9960375.1"
                     /translation="MKTIPRVARRSLRRWLANHLERGASGLGVSLGLLVLACRAEPAK
                     RLFVAASAPALRIEGRVEHSRPGRIRIGFPGVCLKLRFQSRSLKLRASSSSGRNYLSV
                     RVDGGTTRVVHVSQFLRELPLATDLAPGDHEVEIVSRNETWQGVLSVFGFSLDPDGRP
                     LSVAPAATRKLLFVGDSVTCGEGVDRGARCGKAPRDSNAEHSYGRLVAQALSRDGVGT
                     ESHLVCYGGRGLVYDWQGRTDVLNAPQFVDLALPELGAEAWDWSKYVPDAVVISLGTN
                     DFALGRVPAREWFVSEYVTFVRKLRTHAPDAWFLLTEGSIVDAEQKTRLREYLHETVR
                     RLGDRKVSVFEASRQPGTPCDVHPTFEQHHAMAAELLAVLRPLLGASVKTPELRNEP"
     gene            complement(4086..5450)
                     /locus_tag="VFQ61_38055"
     CDS             complement(4086..5450)
                     /locus_tag="VFQ61_38055"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9960376.1"
                     /translation="MSTLHPDSNTIRSLPAPRYAEGRRRGSFLARFGGGPELEVEPGR
                     VRAYPGLRGEAPSMAAYYEAFAEFVKAGRVLDAGSGAGMGTYRLLARGLEVVAVDIDP
                     RAVAFAEQQAPGAEHVVTDLAALEVSEPVDGAILADVLCHTLDPESVLLAIGRALKPG
                     ASLLVAEPRAHVSQRLSAPQRRAFSAARLKSLLLRTGFDVEGVISDRVPFVCLLAKKL
                     DAAKVCEAFGRAYAFSSQGKFNAAVASLQAARAESVVTVATSATLEILLAQFELFLAS
                     GDGDKAASTCFEARELFPNDARPLIGLGRIALASGGASDALSLALEALRHDATEPSAY
                     ALAGVSANELGHPDAFTAFRAAVHLAPDDPLIVQEFVRLCSERGAHSLGLASLDQFER
                     YAVSSGPDQALGAEHHILRASLLIAAGRPNEAEVELRIARMKGADPTELTKLEQALSR
                     GRPQ"
     gene            complement(5450..5851)
                     /gene="fliS"
                     /locus_tag="VFQ61_38060"
     CDS             complement(5450..5851)
                     /gene="fliS"
                     /locus_tag="VFQ61_38060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HET9960377.1"
                     /translation="MFEAAVARYTQIRNTTSTPGELLLALYDGLFRFLNGAKACIEQK
                     DLIRARELLSKAYAIISELYIALDHSVYPELCQNLEGLYGFAMDRITTATRRAEVEPI
                     LEVIQVLTPVREAWRKAVPEAQRENAEANRR"
     gene            complement(5926..7251)
                     /gene="fliD"
                     /locus_tag="VFQ61_38065"
     CDS             complement(5926..7251)
                     /gene="fliD"
                     /locus_tag="VFQ61_38065"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HET9960378.1"
                     /translation="MAGTISFGGIASNMDTEGIVTGLVNASSGQLNKMKTQATETDSA
                     VSTLSSISTLLSTLQRSVDALASTTSVGSYSGSSSSTAIAVSTTGNAQPGSYSIDVKK
                     LAREQRNYSSAVSSSSEALSHSGTLGIKVGSGDQVDIAISATDTLDDIAGKINASGAR
                     ISAAIFNDGSNYRLQIRGLDTGEANAVTFNESGFSLGLNLPESKVQAAQDSEIKIDGF
                     TVKRSTNQVVGALQGVTLALTQETTSPVTVSVQSDPSSLQKKLQSVVDGYNAVITKIH
                     QVAGYGSTKGTVAALQGDSALRSISQRLASAVQTRVSGAGTNDTLGSLGLSLTRDGML
                     SLDGSKLATALNKDANSVAAVLAGPSGGSGVMDIISDLAKGVTRSDGQLTLRQDALTS
                     RADLLRKRADAEKDRLDRYADGLRKQFTQMDGAVAAYNAQSNYLTAFNK"
     gene            complement(7351..8178)
                     /locus_tag="VFQ61_38070"
     CDS             complement(7351..8178)
                     /locus_tag="VFQ61_38070"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HET9960379.1"
                     /translation="MALVLQTNVAALSAQKNLGVNQKSLAGSFQKLSSGFRVTTAADD
                     AAGLAVSESMKSQIRSYTVAERNAADGISMAQTAEGALGEVHGTLGRMRELAMQASNG
                     SLSSTDRSYLDTEFGSLKAEIGRIQDSAKFNGTKLVSNTTGTAIKFQVGLNNTASDQI
                     SVTFGGVALTTLLATASTVSSATAALTALSNIDSAIQTVSNSRAKFGTAMNRLEVATS
                     NIQTMRLNLSAANSRIVDVDVATETAAMSKNQVLSQAGVSVLAQANQLPQLAFGLIR"
     gene            complement(8347..9174)
                     /locus_tag="VFQ61_38075"
     CDS             complement(8347..9174)
                     /locus_tag="VFQ61_38075"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HET9960380.1"
                     /translation="MITIQTNTPSLETQKSLGKTQAQLNRNFSRLSSGFRINTAADDA
                     AGLAISESMKSQIRSYTVAERNSNDALSMTQTAESVLGEMHNILGRMRELAMQGSNGS
                     MTDTDRSYLDTEFTALKEEMQRMQVAAKFNGKQLISSTANSITFQIGLNNTASDQITL
                     TFGGLGLTTLLSTSTTVSGATTGNSLATLSKIDGALTTVSTNRAKYGATMNRLDIAVS
                     SIQTMRLNLSAANSRIRDVDVAEETASMSRNQILSQAGASVLAQANQLPQLALSLLK"
     gene            complement(9171..>9837)
                     /locus_tag="VFQ61_38080"
     CDS             complement(9171..>9837)
                     /locus_tag="VFQ61_38080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960381.1"
                     /translation="AWGGRGRVISTIGFSAVRAWLEAASIVLSREPSPPRMVNATEGG
                     ARIAGFEEECLASLLESLPDCEIHSEELVRHARERARQPEGSEIAAWARAQADLVGRA
                     RRIARRIARLSRVAEQAAKQGKTSMSRKLTRLEQSERELAQSVAEAPLLDAWSFAAVD
                     HLMDRHEATTEGSHAQAARGFAFEARLGETIEDSARQLQHELLQLSDRLTHSPLPHRR
                     EQP"
BASE COUNT         1744 a         2941 c         3203 g         1949 t
ORIGIN      
        1 tcgaccgcat aagacagcga ggcctggccc cgagcaaccc tgcggactgg aaacgcgccc
       61 tgaacgatgc cggtgtacat gggaacggct caggatagct gccgcccggc gacgagcaaa
      121 atctgccgga gtcaccggtt ccgctcgaac ctctcgagtt cagacttttg tttcggcggg
      181 ctgtggtggg agctggggtc gccgggtcca acgtggcagc aaactggaga cgagcgcgag
      241 cgcggctaag ctccagaacg gcacccgata acttcccgag acctcgtaga gcgctgccgc
      301 gagcaaggga ccgacgaccc ctcccaagct ccaggcgacg atcatcgccc cgtaaatgga
      361 cccggcgtgt ttggtaccga agaagtcagc cgaggcggcg ggcatgacgc caaagcctcc
      421 tccgtaactc gtgtatacga gggcggctag gatgaagaac aacacggcgc cgtgcgccag
      481 cggcagtagc gccagggcca cggcctgact gaaaagcaac cagctgcaga ccgacatgcg
      541 acccaggcga tcggagagac cggcgtagaa tacgcgcccc gcaccgttga acaacccgaa
      601 gatgccgacc aacgctgccg cggcacttgg gccaagctga ccgagctcct gagccgcgtc
      661 agaggcttgg ctgatgaggc cgatgcccgc ggtgacgttg agcagcaaga tcgccgtgag
      721 ttgataccag gctggagtcc tcaaggcctg ggacagcgtg agctcgcgct gcccgagttg
      781 cggagcctgc ggcccacctt taggccccgc gccgcccttg ttcgacgacg gatttcggaa
      841 cgagagcgcg cccaacgtga tcagaaccag ataggcgagc ccgaggggca agaacacact
      901 tggcttgtta cccgcgttcg cgagcaggct ccgtgcaagt ggtgccgcca gcacggagcc
      961 gaaaccaaat ccgcccacgg cgagcccggt gaccaggccg cgtcgctcgg gaaaccactt
     1021 ggcgagcatc gcgatggggg tgatgtagac cgccccgagc ccggtaccgc cgatcacgcc
     1081 ataacccagg gtgagcagcc acaattggtc ggcgcggtgg acgcatgacg cgagcatgat
     1141 ccccgcggcg tacaagaagc caccaatcaa ggcgacgggt cgtgggccgt gacgatcttg
     1201 agcccggcca cccagcaagg ccccaatcac gatgcatccg attgccgtct cgaatggcag
     1261 caccgtttgc ggtttggtcc acccgaactg ggtctggaga ggtttattga agacgctcca
     1321 agcataaacc gatccgagcg cgagctggac cagaaccgcg gagatcagaa tgcgaactcg
     1381 tggtccttgg tcggccgctt ctagcgcgga ctcctcgtga ctcgcggttc gctcggggcc
     1441 atcgggcggc gttggtttct cgaggcttga ttcctccgac actgattcat cgttcgagcg
     1501 cgattctcct gaacgcgagc tcgagagaga cggcgctgcg tcggatgcgg gcggtgcgga
     1561 cgagtcagcc ttcatgcgtc gatgcttatg ccacgaattg cccgcggttc gctgccctcg
     1621 tgtgggctcg ggatcacgac ggacaaaaag aaagcggcga agtagcccaa cttggttggg
     1681 gcagcttcgc cgctttcacg aacgccagcg ccgactacag agcatcagca ggtatgactg
     1741 gggctcccat tggggccgcc aaggggtttg ctgtgtctta acgtgtgctg gcttgcctag
     1801 cgtagcctac gaattcagga cacgcgattg atcaccgtca gtcgagtgat cgcgatcaac
     1861 tcagtcatca gactcggccc attacactcg ttgatcgatc ggactctatt tgatcagtgg
     1921 aagtcgcgga tcttccaatc aggccgctct actcacggtc tccgtttcat tcggtttcgg
     1981 tttgcggcgc tgaatctacg gtaactctgc tttcgaactg acgggcctta gcttcggata
     2041 gacgggcctc acttgctcgt aaccggcacc ttctgcgggg ggctcacggc tttcttgggg
     2101 acgcggacca tcaacacgcc actcttgagt tcggcgcgaa cggcgttcgc gtcggtctcg
     2161 tgtggaaggt tgaaggctcg tgaaaagtgg ccgtaggaac gttcgtacgt gtaataggtg
     2221 tcggtcttct cgttgcgttc gctctcacgc ttgccagaga tggtgagacg attctgattc
     2281 aagctcactt ccaagtcgtc cggtttgacg cccgggagat ccgccgtgaa gacgaactct
     2341 tcgggggtct ctttgatttc gacggctgga gtaaagccag actcggagaa cccgctccac
     2401 aggggagcca ttctggcgaa ggggtcccag cgcgcaattt cacgcaaggc acggaaagga
     2461 tcccactcac gatacgtgct cggcgtctgc gacgccgggg cgttcggatc tttctggacg
     2521 gcaatgttgg tcatggctca tccgctcctt tctatggctt ggccttttct gtactggctg
     2581 cctttctctc gcttcgccct gccccgacta cgcgcatcct ggcggagttc gcgagtactc
     2641 gcgtccttcg ttttggccag cgctgagtcg agactttgaa gtgagtgggt cgttgtgact
     2701 gaacgccgag gagtagagca agagctattc cacaggtaaa ttgcgctaga tcggcggcat
     2761 tgaagcaccg gctgccgctg attcgagcaa atattgcgat gattcttgga aagccggcag
     2821 cgcgtgcgcg atagggcttc acgaatggcc cagtcccggg ttctagttaa atcctcgtga
     2881 aggctccctg ttcatgaaga cgatcccaag agtggcacgg cgttcgctac ggagatggct
     2941 cgcaaaccat ctcgaacggg gtgcttcggg cctcggtgtg tccttgggct tgctggtgtt
     3001 ggcgtgtcgc gcggaacccg caaagagact cttcgttgcc gcgagcgcgc cggccctgcg
     3061 tatcgagggc cgcgtcgaac attcgcgacc tgggcgcatt cggattggct ttcccggagt
     3121 ctgcctgaag ttgcgttttc agagccgctc tttgaagctc cgagcctctt cgagttcagg
     3181 tcggaattac ctttcggtgc gggtggatgg cgggacgact cgcgtcgtgc atgtcagcca
     3241 atttttacgc gagctgccgc tcgccacaga cctggctccg ggtgatcacg aggtagaaat
     3301 cgtgagtcgg aacgagacct ggcaaggggt gctttccgtc ttcggattca gtttggaccc
     3361 tgacggtcgc cccttgtcgg tggcgcccgc ggcgacgcgc aagctgctgt tcgtgggtga
     3421 ttcggtgacg tgtggagaag gcgtggatcg tggggctcgc tgtggaaagg ctccccgcga
     3481 ttccaacgcg gaacactcct atggacggct ggtcgcgcag gcgctcagtc gagatggcgt
     3541 ggggaccgag agccatctcg tgtgttacgg cggacgaggc ctcgtttacg attggcaagg
     3601 cagaaccgat gtcctgaacg ctccgcagtt cgtagatctc gcgctgccag agttaggcgc
     3661 cgaggcctgg gattggtcga agtacgtgcc cgacgccgtg gtgatctcgc tggggaccaa
     3721 cgatttcgct ctgggccggg ttccggcacg tgagtggttc gtctcggagt acgtcacgtt
     3781 cgtgcgtaaa ctccgcaccc acgccccgga tgcctggttc ttgctcaccg aaggctcgat
     3841 cgtcgacgcc gagcagaaga cccgtctacg tgaatacctg cacgagacgg tgaggcgtct
     3901 cggtgatcgg aaggtgagcg tattcgaggc ctcccgccaa ccaggaacgc cttgcgacgt
     3961 ccatcccact ttcgagcagc atcacgccat ggcggccgag ctattggccg tgttgcgacc
     4021 gttgttgggg gcgtccgtca agacgcctga acttcgcaac gagccttgaa ggcgtgggcg
     4081 cgcctctatt gcggacggcc tcgcgacagc gcttgctcga gcttcgtcaa ttccgttgga
     4141 tcggcccctt tcatgcgggc aatccgcagc tcgacctcgg cttcgttggg acgaccggcg
     4201 gcgatcagca acgaagcgcg aagtatgtgg tgctccgcgc cgagcgcctg atccggcccg
     4261 gaactcacgg cgtagcgttc gaattgatcc aggcttgcca agccgagcga gtgcgctccg
     4321 cgttcagagc agagccttac gaactcttgg acgatcagcg gatcgtccgg cgcgagatgc
     4381 accgcggccc ggaacgcggt gaaagcgtcg ggatggccga gctcattcgc cgagacaccc
     4441 gccagagcgt acgcactcgg ctcggtcgcg tcgtgccgca acgcttcgag tgccaagctc
     4501 agtgcgtccg acgcaccgcc cgaagcaagg gcgatgcgac ccaatccgat cagcgggcga
     4561 gcgtcattcg ggaacaactc gcgagcctcg aagcacgtgc tggccgcctt gtctccgtca
     4621 ccagaggcca gaaatagctc gaactgggcc aacaagatct cgagcgtggc gctggttgcc
     4681 accgtgacga cgctctcggc gcgggccgct tgcaggctcg ctactgctgc attgaacttg
     4741 ccctgcgagc tgaaggcata ggcgcgaccg aaggcctcac acaccttggc cgcgtcgagc
     4801 ttcttagcca acaaacagac gaacggcacg cgatcggaga tcacgccctc gacgtcgaag
     4861 cccgtccgga gcaacaacga cttcagacgc gcagcagaaa acgcgcgtcg ctggggcgcc
     4921 gacaaacgct gagagacgtg ggcgcgaggc tccgcgacca gcagcgaagc gccgggcttc
     4981 agcgcacggc ctatggctag cagcaccgat tccgggtcca acgtgtgaca caacacgtcc
     5041 gctaggatcg cgccgtcgac cggctccgac acttctaggg ctgcgagatc cgtgacgacg
     5101 tgttcggccc ctggggcctg ctgctccgcg aaagcgacgg ctcgcggatc gatgtcgacc
     5161 gccacgactt ccagcccgcg cgcgagcagg cgatacgtgc ccatgcccgc tcccgagccc
     5221 gcgtccaaga cgcgacccgc tttgacgaac tcagcgaagg cctcgtagta ggccgccata
     5281 gatggcgcct caccgcgtag gcctgggtac gcgcgcacgc gtccgggctc aacctcgagt
     5341 tctgggccgc caccgaagcg agcgagaaag ctgccgcgcc gtcgcccttc agcgtaacgt
     5401 ggcgcgggta agctacgaat cgtattggaa tctggatgca gcgtggacat cagcgacgat
     5461 tggcctcagc gttctcacgc tgggcctccg ggacggcttt gcgccaggct tcgcgcaccg
     5521 gcgtaagcac ttggatgact tccaggatgg gctccacctc cgcccgccgc gtggccgtgg
     5581 tgattcggtc catcgcaaac ccatacagcc cttccaggtt ctgacacaac tccgggtaca
     5641 ccgagtgatc gagcgcaata tagagctcgg aaatgatggc gtacgctttg gagagcaact
     5701 ctcgtgctcg gatgaggtcc ttctgctcga tgcaggcttt ggccccattc agaaaacgaa
     5761 acaggccatc gtacagcgcg agcaagagct cgccaggcgt actggtggta tttcggatct
     5821 gtgtataacg ggcaacggcg gcttcgaaca tggaacatca atccgaaccc gaaggttcgt
     5881 gtctctatgg aaaagccagt cttggggact ggcgggctga aggggttact tgttgaatgc
     5941 ggtgagatag ttggactgag cgttgtaggc ggcgacggcg ccgtccatct gcgtgaactg
     6001 cttgcgcaag ccatccgcgt agcggtctag ccgatctttc tcggcgtccg cgcgtttgcg
     6061 cagcagatcg gcgcggctgg tcagggcatc ctggcgcagg gtgagttgac catcgctgcg
     6121 agttacgcct ttggcgaggt cgctgatgat gtccatgacg ccgctgcccc cactgggtcc
     6181 ggctaggacg gcggccacgg agttggcatc cttgttgaga gcggtagcga gcttgctccc
     6241 gtctagactg agcatgccgt cgcgggtgag ggagagaccg aggctaccca gagtgtcgtt
     6301 cgtcccggcc ccgctcacgc gcgtctgcac ggcgctcgcg aggcgttggg agatggaccg
     6361 cagagcggag tcaccctgca gagccgcgac cgtacccttg gtggagccgt aaccggcgac
     6421 ttggtgaatc ttggtgatga cggcgttgta gccgtcgacc acggactgga gcttcttctg
     6481 gagtgagcta gggtcgctct gcaccgagac cgttaccgga ctcgtggttt cttgggtcag
     6541 ggccagggtt acgccctgga gcgcgccgac cacttgattc gtcgagcgct tgacggtgaa
     6601 gccgtcgatc ttgatttcgg aatcctgggc ggcttggacc ttggattcgg gcaggttcaa
     6661 gccgaggctg aatccggatt cgttgaacgt gaccgcgttg gcctcgccgg tgtcgagccc
     6721 gcggatttgc agccggtagt tggaaccgtc attgaagatg gcggcggaga tgcgcgctcc
     6781 ggaggcgttg atcttgcccg cgatatcgtc gagcgtgtcg gtcgcgctga tcgcgatatc
     6841 cacttggtct ccgctgccga ccttgattcc cagcgtgccc gagtgactca gcgcttcact
     6901 cgaggacgag acggcgctcg agtagttgcg ctgctctctg gccagcttct tcacgtcaat
     6961 cgagtaagag ccgggctggg cgttgccggt cgtgctgacg gcgatggccg tgctggaaga
     7021 actaccggag tagctcccga cgctggtcgt gctcgcgagg gcgtccaccg agcgctgtaa
     7081 cgtggagagc aaggtgctga tgctcgataa ggtcgagacg gccgagtcgg tctcggtcgc
     7141 ctgcgtcttc atcttgttca gctgcccgct cgaggcgttg accagtccgg tgacgatgcc
     7201 ttcggtgtcc atattggacg caataccgcc gaatgaaatc gtgcctgcca tgtcgtgcct
     7261 cgcgttaaag gggagcggtt agctgagtcg ggccgtggcg gcctctctga agctcatcgg
     7321 aggagccgcc acgacttcca ccgactgcta ttaacgaatc aggccgaacg ctagctgggg
     7381 cagctggttg gcttgggcca acaccgacac acccgcttgc gagagcacct ggttcttgga
     7441 catggccgcg gtctcggtcg ccacgtccac gtccacgatg cgggagttgg cggccgacag
     7501 attcaagcgc atcgtctgga tgttgctggt ggcgacctcg agacgattca tcgcggtacc
     7561 gaacttggcg cgcgagttcg acacggtttg aatggccgag tcgatgttgc tgagcgcggt
     7621 cagcgcggcg gtcgcgctcg atacggtgct ggccgtagcg aggagggtgg tcagggccac
     7681 gccgccgaag gtcaccgaaa tttgatccga ggccgtgttg ttgaggccga cctggaactt
     7741 gatcgcggtg ccggtggtgt tggagacgag cttggtgccg ttgaacttgg ctgagtcctg
     7801 gattcggcca atttccgctt tgagcgagcc gaactccgtg tcgaggtagc tgcggtcggt
     7861 cgagctcaag ctgccgttcg aagcttgcat ggctagctcg cgcatacgcc cgagcgttcc
     7921 gtgcacctcg ccgagtgcac cttccgcggt ctgcgccatc gagatgccgt ccgccgcgtt
     7981 gcgctccgcg accgtgtagg agcggatttg cgacttcatg ctctcgctga ccgcgagacc
     8041 tgctgcgtcg tcggcagcgg tggtgacccg gaagccgctc gacagctttt gaaagctgcc
     8101 ggcgaggctc ttctgattta cgccaaggtt cttctgtgcg gatagagctg ctacgttggt
     8161 ttgaagtacg agtgccatgg gagactcctt cctggagagc gacccggcgc ttgccgggtc
     8221 cgggcttcat tgccctttgc tccttcagga acggaggggt cgactggacc ttgaataaaa
     8281 actccgatga acttcgtacc ctacgtacgg ctcccgccac ctcacgcgca gccagcatgg
     8341 cgccacctac ttgaggaggc tgagtgcgag ctgcggcagc tgattggcct gggcgagcac
     8401 cgaagctccc gcttgggaga ggatttgatt gcggctcatg gaagcggttt cctccgccac
     8461 gtctacatcg cggatccgtg agttggcggc cgacaagttg agacgcatgg tctgaatcga
     8521 cgaaacggcg atgtccaagc gattcatggt cgcaccgtat ttggcgcggt tggtggacac
     8581 cgtcgtcaag gcgccgtcga tcttgctgag tgtggccagc gagttccccg tggtagctcc
     8641 cgataccgta gttgacgtcg agagcagggt ggtcaaaccg agcccgccga acgtcagagt
     8701 aatctgatcg gatgccgtgt tgttgagacc gatctggaag gtgatcgagt tggccgtcga
     8761 ggagattagc tgcttgccgt tgaatttcgc tgccacctgc atacgctgca tctcctcctt
     8821 gagcgccgtg aactcggtgt cgaggtagct gcgatcggtg tcggtcatgc tgccgttgga
     8881 gccttgcatc gcgagctcgc gcatgcgccc caggatgttg tgcatctcgc cgagcacact
     8941 ctccgcggtc tgcgtcatcg acagcgcgtc gttgctgttg cgttccgcga ccgtgtaaga
     9001 acggatctgg ctcttcatac tctcgctgat ggcgagaccc gcggcgtcgt cggcggcggt
     9061 gttgatacgg aagccactcg ataggcgcga gaagttgcgg ttcagctggg cttgcgtctt
     9121 gcctaatgat ttctgggttt cgagagacgg ggtgttggtt tggattgtga tcatggctgt
     9181 tctcgtcggt gcggtagcgg gctgtgcgtg agccggtcac tcaattgcag cagctcgtgt
     9241 tggagctgcc tggctgaatc ttcgatcgtt tcgccgaggc gggcctcgaa ggcgaagccg
     9301 cgtgcagcct gggcatgcga accttcggtg gttgcctcgt ggcgatccat caagtggtcg
     9361 acggcggcaa aagaccaagc atctagcaag ggagcttccg ccacgctctg cgcgagttcg
     9421 cgttcgctct gctcgagacg cgtgagcttg cggctcatgg aagtctttcc ctgtttggcg
     9481 gcctgctccg cgactcggga taacctcgcg atacgccggg cgatgcgccg ggcgcgcccg
     9541 actaggtcgg cttgtgcgcg cgcccaagct gcgatctccg agccctcggg ttgacgtgcg
     9601 cgctcgcgag cgtggcgcac gagctcttcc gagtggatct cacaatccgg caagctctcg
     9661 agcagggagg cgaggcactc ctcctcgaag ccggcgatgc gagcgccgcc ttcggtcgcg
     9721 ttcaccatgc gcggtgggct gggctcgcgc gacagcacga tgctcgccgc ttcgagccag
     9781 gctcgcactg cggagaatcc aatcgtcgaa atcacgcggc cgcggccgcc ccaagct
//
LOCUS       DASTKU010001073        11832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_2001165, whole genome shotgun sequence.
ACCESSION   DASTKU010001073 DASTKU010000000
VERSION     DASTKU010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11832
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001165"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..363)
                     /locus_tag="VFQ61_38085"
     CDS             complement(187..363)
                     /locus_tag="VFQ61_38085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960382.1"
                     /translation="MRNGFGRASGDGGLPAPDSPPSANKSETQVRPHSDRRMSPLTYN
                     SQLGRAPSRRAKLI"
     gene            complement(568..1545)
                     /locus_tag="VFQ61_38090"
     CDS             complement(568..1545)
                     /locus_tag="VFQ61_38090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HET9960383.1"
                     /translation="MNRWNRFDRFEATPKKPPPERGIRMKKAGTTWWGQRWIQALQSV
                     LSGDAGRLARGRTYARAGRTHDLIVQAGKVTAKVTGSRATPYEITIELVELTAPVWQK
                     AIEGLAQKAQFSAELLAGQMPRAIDEVFISAGESLFPTRRADLETKCSCPDWGDPCKH
                     VAATHYVLGEALDRDPFLLFELRGRTKDEVLGALRAARGAASEPPAGKGPGRESTPAE
                     GGTEVPAVRLGQLNPGEYDKPRGPLPNLHFSFDEPVSHGAVLRQLGAPDAWASNITPA
                     DTLSPLVQAAAETARRIAMSEPSEVASSRLPPKKRSARARKRKHQGRDE"
     gene            complement(1542..4574)
                     /locus_tag="VFQ61_38095"
     CDS             complement(1542..4574)
                     /locus_tag="VFQ61_38095"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9960384.1"
                     /translation="MAVPRGMYRLPMPLAYVPSVESLFLWGAEPVPRAVPALAEGGEP
                     WTMTLLAPEGLREAVGVKLPLIETMTQLAVVPTVDVESLPGSVAIWVLASKLAIDLVA
                     RERVVPTITRRGDRIEARWAAALSSSEDAAKLAALARSMPPAAHAVPAAGALTGVVWA
                     PDALLRAYLDAVVDALVRAARGGPSTPAAQPKATRAASNSWTERWQRALGSGERSFET
                     QGFAERSLVDELVRWSEPALGARDRLRACFRLELPTSDGEPFLLRFLLQSPDDPSLLV
                     AASEVWKTTGRSLEKFGRAFRDPQECLLEALGRAGRLFPALARSLEEARPESVALDPA
                     TAWGFLGEGAASLAEAGFGVIVPGELTATGQRRLRLRMRVGGSKRKVAGVVAGGAGLA
                     LDELLSVDWEAMIGDEPLTAGELAALAKQKAPLVQFRGAWVAIDPRELQGIQKRLEEG
                     TSKLTAAEAIPAVLAGEARVGELSVAVAASGAVAELVERLRSGGARELEAPRALRATL
                     RSYQQRGLNWLVTMGSLGLGACLADDMGLGKTMQLLAFLLRRMERVPDDERPALLIAP
                     TSVIGNWEREVERFAPCIEVVRHYGASRAKSEGDIPRRPGTLVVTTYGLLRRDAELLA
                     SIDWSVATLDEAQNIKNAASATARAARALRASHRFALTGTPVENRLAELWSILEFANP
                     GLLGPLPNFRKDFAIPIERYGNEPAATRLRRIVGPFILRRLKSDPTVIQDLPPRNEMK
                     VVCTLTREQATLYKAVVDEELRRIDSSEGMERRGRVLALLMFTKQICNHPAQYLGESG
                     PLPKRSGKLARTTEMLEEAIAAGDKALVFTQFREMGDRLLEHFGAKLGCEVVFLHGGT
                     PKKARDELVRRFQEEPHGPRVFVLSLKAGGTGLNLTAANHVFHYDRWWNPAVEDQATD
                     RAYRIGQKRAVQVHKLVCAGTVEEKIDRLLEQKRELASKVIGAGEQWITELDGRELRD
                     LFSLSEGAVADGDDEDVGSPAPSEPRAKKTRSRREVRP"
     gene            complement(4827..5168)
                     /locus_tag="VFQ61_38100"
     CDS             complement(4827..5168)
                     /locus_tag="VFQ61_38100"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9960385.1"
                     /translation="MHLGGAARCWTLRDEQNARRRENATRITANGVSERTLHRLLVAE
                     TGLSFVRWRQQLHIVLAIQKLSRGAAVQNVAVELGYESASSFVTMFRKAVGSSPARYM
                     AHRLASAPKRT"
     gene            complement(5424..6749)
                     /locus_tag="VFQ61_38105"
     CDS             complement(5424..6749)
                     /locus_tag="VFQ61_38105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960386.1"
                     /translation="MRTALLAFVGLTISGVGCSSSSPEPGMQPGNSGGASSGAGGAKV
                     TGGAAATGGQTSPGQATQGGSTAAQGGQTASGGASSATGGANGGTNAAGSGTGGAAGG
                     VMNTAGAGTGGAGVAGSGMGGASSGGMGMGGGSATSGGKGGAGGMSSAGATSSGGKSG
                     GTGGATGSGGQSSGTCTASKDTGKSASGSGPHKVVIETNSENGIKCGTIYRPKELGGA
                     EKYPILVWGEGGCSQDGLSNQAAMSEIASWGYFVIADGTPGASNACQGGQNGKAFLDY
                     ITWAIAENDKSCSAYYQSIETTKIAADGFSCGGLMAENVSGDPRFSAIGFTSSGLMSA
                     NASLYDKIHTPFKIMNGGSSDIAYENGLRDYNEISKRGVPIVYFSKTSAGHGGDLGQA
                     RGDFNKVNLAWLNWQLKGDMGATGKGFLYGSSCGFCTNSGWVYKSANIE"
     gene            complement(7019..8248)
                     /gene="zigA"
                     /locus_tag="VFQ61_38110"
     CDS             complement(7019..8248)
                     /gene="zigA"
                     /locus_tag="VFQ61_38110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallochaperone GTPase ZigA"
                     /protein_id="HET9960387.1"
                     /translation="MHSASRRLPVTVLSGFLGAGKTTLLNHVLANREGRKVAVIVNDM
                     SEVNVDARLVKTGGAALSRVDEKLVEMQNGCICCTLREDLLVEIGRLAREGRFDYLLV
                     ESTGISEPLPIAETFTFEDELGQSLSQVARLDTLVTVVDAKNFLTDWHSEEDLRARKI
                     ALGEDDERSLPDLLVEQVEFANVLVLSKLDLVSTADAERLASMLRHLNPEARIVHADN
                     GKVPLDAVLGTDLFDFEAAARAPGWLKELRGEHVPETLEYGISSFVYRARVPFHPDRF
                     WTLLQDPDTWKDVLRSKGFFWLASRMEVAGLWSQAGSSASCEPAGMWYSAMPEGSWPD
                     DDESRRQLERDWQEPWGDRRQELVFIGARINERALRRRLDSALLTETELEGGPNLWQE
                     LQDPFTAWPTSSPLVED"
     gene            complement(8684..9130)
                     /locus_tag="VFQ61_38115"
     CDS             complement(8684..9130)
                     /locus_tag="VFQ61_38115"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET9960388.1"
                     /translation="MAENSVKLHRVLRAPAERIYRAFLDPDAMAKWLPPHGFTGRVHH
                     IKAEVGGTYKMSFTNLTTGHSHSFGGEFLELVPNERIRHSDKFDDPNLPGQMQTTISL
                     KKVSVGTELTVLQEGIPSIIPVEACYLGWQESLTLLTQLVEPEIKE"
     gene            complement(9391..10113)
                     /locus_tag="VFQ61_38120"
     CDS             complement(9391..10113)
                     /locus_tag="VFQ61_38120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET9960389.1"
                     /translation="MQVARFVEEVRVYESTPGQRHRIDRLPDGRTLFVFRKLAENHGD
                     LSVAGPRTQAYTKEAGGIQRAVVIQIKPGWAPAMLGVGARRLTDHVVSLEEIWGSSAR
                     SLLADLLSTGSADAAIERITHAFATRLPLISESASARLARRAVLLLEEQETSIESLAA
                     RLGVTARHLRRAFTDTVGIGPKEFARSARLRRAVRHTSTSHTLGRIAADAGYYDQAHL
                     ARDFRELLGVTPSSYVKHGLAR"
     gene            complement(10284..10934)
                     /locus_tag="VFQ61_38125"
     CDS             complement(10284..10934)
                     /locus_tag="VFQ61_38125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET9960390.1"
                     /translation="MPNVEPGLRERKKAKTRLAISRIATELFNERGFEAVTVAEIAAA
                     AEVSVATIFNYFETKEDLFFDREGEVIAAHRRFVREREPGESIPGALHRAFRAAIDGD
                     TLSHLVASGGRFVRTLETSPSLRARARFALEKTEAALTEAISEATRVAKGDPTPRAVA
                     AMILAIERVLMEDARAAVLRGDSPAKMKRGLRRTCDRAFELLDSGVRDYGRKACER"
     gene            11042..>11832
                     /locus_tag="VFQ61_38130"
     CDS             11042..>11832
                     /locus_tag="VFQ61_38130"
                     /inference="COORDINATES: protein motif:HMM:NF028194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HET9960391.1"
                     /translation="MPPTTQRRNRQWLLKTRPHGALSLDNFEFRDQEMPEPELASGQI
                     LVKNLLFTCAPTMRNWLNAPGNSHRTVNPGTPILGPGGSEVIKSNHPRFPVGTRVTLL
                     SRWEDYSVLEPDASPTPVIAHSSDVGLSQAMGILGLNSCTAYFGLLSVGQAKAGETVV
                     VSAAAGSVGSMVVQIAKITGCHVVGIAGGGEKCRLVVDEFGADAAIDYKHENVAVRLG
                     ELCPRGIDVFFDNVGGEILDAVMDNIAFKGRIAVCGQVSAYDSGTL"
BASE COUNT         2033 a         3988 c         3592 g         2219 t
ORIGIN      
        1 cgtaggttca gcggcgcacc tgagccccga gattacgggt ggacatacta gagtctgtgt
       61 tccaggcgtt gcgatccgat cactgcgatc cactcctcga tccaatcctc gttctgggcg
      121 gatcgccgat cggccgtcgc catcatcttg cgtcagtggt gtcgcaacgt caccggaacc
      181 tcgacttcag atcagcttgg cgcgacggct cggtgctctc ccgagttggg agttgtaggt
      241 cagcgggctc atcctgcgat ccgagtgtgg tcgaacctgc gtttcgctct tgttggcgga
      301 agggggcgag tctggagctg gcaaaccacc atcacccgac gcacgaccaa acccgtttct
      361 catgaccact caaggtatca cgaggaggca ggcaccaagc tcttcagtcc actcggtata
      421 acaattgtgt gtgatagcac cgcacctgat cgggcatcat ttcttcgctt gggcgtcgcg
      481 gagatctctc agcccgagag ctcgcgagct cgcgctcgtc agcccggcga cccgcgtctt
      541 gtctgagccc gggttcctgg ggttggctca ttcgtctctg ccctgatgct tccgcttccg
      601 cgctcgagcg ctgcgctttt tcggcgggag ccggctcgat gcgacttcgc tcggctcact
      661 catcgcaatc cggcgtgcag tctctgcggc tgcttgcacg agggggctga gagtgtcggc
      721 gggtgtaatg ttacttgccc acgcatcggg cgcccccagt tgtcgcagca ccgcgccgtg
      781 gctgacgggc tcgtcgaagc tgaaatgcag attggggagc gggccgcggg gcttgtcgta
      841 ttcacctgga ttcagctgcc cgagcctcac cgctggaacc tccgtgccgc cttctgcagg
      901 cgtcgactca cgcccggggc ccttgcctgc cggaggttcg ctcgccgctc cgcgcgccgc
      961 acgtagggcg ccgagcactt catccttggt tcttccgcgg agctcgaata acagaaacgg
     1021 atcacgatcg agcgcctcgc caagcacgta gtgcgtcgcg gccacgtgct tgcaaggatc
     1081 tccccagtct gggcacgagc acttggtctc gaggtcggcg cgccgcgtcg ggaagaggct
     1141 ctcacccgcg gagatgaaga cctcgtcgat ggctcgaggc atctgccccg cgagcagctc
     1201 ggccgagaac tgcgccttct gggccagccc ttcgatggct ttctgccata cgggagcggt
     1261 gagctcgacg agctcgatgg tgatctcgta cggcgtcgcc cgtgaacccg tgaccttggc
     1321 ggtgactttg ccagcttgga cgatgaggtc gtgagtgcgc ccggcgcgcg cgtaggttcg
     1381 accgcgtgcg agccgcccgg cgtcgccgct caagacactc tgcaacgctt ggatccatcg
     1441 ctggccccac cacgtcgtgc ccgccttctt catacggatg ccccgctcgg gcggcggctt
     1501 cttcggggtg gcctcgaatc gatcgaaacg attccaacga ttcatgggcg cacctcgcgg
     1561 cgggagcgcg tcttcttcgc gcggggctcg gaaggggcag gggaacccac gtcctcgtca
     1621 tccccgtccg ccacagcgcc ctcggacagt gagaagagat cgcgaagctc ccggccatcg
     1681 agctcggtaa tccactgctc gccggcaccg atcaccttgc tggccagctc acgtttctgc
     1741 tccagtaatc gatcgatctt ctcctccacg gtgcccgcgc acaccagttt atggacctgc
     1801 acggcgcgct tttgcccaat gcgataggcg cgatcggtcg cctggtcttc cacggcgggg
     1861 ttccaccagc ggtcgtaatg aaagacgtgg ttcgccgccg tgagattgag accggtgccg
     1921 ccggccttca aagagagcac gaacacgcgg gggccgtgcg gctcctcttg aaagcggcgc
     1981 accagctcgt cgcgcgcctt ctttggcgtg ccaccgtgaa gaaacactac ttcgcagccc
     2041 agcttcgcgc cgaagtgctc caacagacgg tcacccattt cgcgaaactg cgtaaacacc
     2101 agagctttgt ctccggccgc gatcgcctct tccagcatct cggtcgtgcg tgccagcttg
     2161 cccgagcgct tcggcagggg gccgctctca cccaggtact gggcggggtg gttgcagatc
     2221 tgcttggtaa acattagcag cgcgagcacg cgtccgcgcc gctccatgcc ctccgaggag
     2281 tcgatgcgcc gcagctcttc gtctaccacg gccttataga gggtggcttg ctcgcgcgtc
     2341 agcgtgcaca ccaccttcat ctcgttcctg ggcggaagat cttggatcac ggtcgggtcg
     2401 ctcttgagcc gccgtaggat gaacggccct acgatgcgac gcagtcgcgt cgccgccggc
     2461 tcgttgccat aacgctcgat ggggatggcg aagtccttgc ggaaattcgg aagcgggccc
     2521 aatagacccg ggttggcgaa ctcgaggatc gaccacagct ccgcgagtcg attttccacc
     2581 ggggtgcccg tgagcgcgaa gcggtggctg gctcgcagag cgcgggccgc gcgggcggtc
     2641 gccgaggcgg cgttcttgat gttttgcgcc tcatcgagtg tagccacgga ccaatcgatg
     2701 ctcgccaaga gctcggcatc gcggcgcagc agtccgtagg tcgtcaccac gagcgttccc
     2761 ggtcttctag ggatatcgcc ttccgatttt gctcgcgacg cgccgtagtg acgaacgacc
     2821 tcgatgcaag gcgcgaagcg ctcgacctcc cgttcccaat tgccgatcac ggaggtcggg
     2881 gcaatcagta gcgccggccg ctcgtcatcc ggaactcgtt ccatacgccg caggagaaaa
     2941 gccaggagct gcatcgtctt accgagaccc atgtcgtcgg cgaggcacgc gccgagaccg
     3001 agcgagccca tcgtaacgag ccagttcagc ccgcgctgct gatacgagcg tagggttgct
     3061 ctgagcgcac gcggcgcttc gagctcccgc gcaccgccgc tccgcagccg ctccaccagc
     3121 tctgctacgg ctcccgacgc ggccaccgcg accgaaagtt ctcccactcg tgcttccccg
     3181 gcgagcacgg ctgggatcgc ctccgccgcc gtcagcttcg acgtgccttc ctccaggcgc
     3241 ttttgaatgc cctggagttc gcgcggatcg atcgccaccc acgctccacg aaactgcacc
     3301 agcggcgcct tctgcttggc aagcgccgcg agctcccctg cggtgagtgg ttcgtccccg
     3361 atcatggctt cccagtccac cgaaagcagc tcatcgagtg cgagtcccgc gccacctgcg
     3421 accacgccgg cgactttcct tttacttcct cccacccgca tacgcagccg caggcgacgc
     3481 tgcccggtgg ccgtgagctc tcctggaacg atcacgccga agcccgcttc agcgagcgac
     3541 gccgcacctt cgccgagaaa cccccaggcg gtagcaggat ccagcgccac cgactcgggc
     3601 cgcgcctctt cgagcgagcg cgcgagcgcc ggaaagagtc gtccagcgcg cccgagcgcc
     3661 tcgagcagac actcctgcgg atcacgaaac gcacgcccga acttttcgag actccgcccc
     3721 gtggtcttcc acacctccga agcggccacg agcaggctcg ggtcatcggg agactgcaac
     3781 agaaagcgga gcaagaacgg ctctccgtcg ctcgtcggca gctccaagcg aaagcacgcg
     3841 cgcagccggt cgcgcgctcc cagcgccggt tcgctccaac gcacgagctc gtccactagg
     3901 gagcgctctg caaaaccctg cgtttcgaag ctgcgctcgc cgctgccaag cgccctctgc
     3961 cagcgttcgg tccaggagtt cgaggcggct cgtgtcgcct tcggctgcgc tgctggcgtt
     4021 gacggccctc cgcgggcggc gcgaaccagc gcatccacca ccgcgtcgag ataggcgcgc
     4081 aacagcgcat cgggcgccca cactacccct gtgagagccc ctgcggccgg caccgcgtgc
     4141 gccgccggag gcatactcct ggcaagcgcc gccagcttcg ccgcgtcctc gctgctcgag
     4201 agagcagcgg cccaacgcgc ctcgatccga tcaccccttc gagtaatggt cggcacgacg
     4261 cgctcgcgcg ccacgagatc gatcgcgagc ttgctcgcca gaacccaaat cgccaccgaa
     4321 cccggcagac tctcgacatc gaccgtcggc accaccgcca gctgggtcat cgtctctatc
     4381 aacgggagct tgactcccac cgcttcccga agcccctcgg gtgccagaag cgtcatggtc
     4441 caaggctctc cgccctccgc cagcgccggc accgcgcgcg gcacgggctc tgcgccccac
     4501 aggaacagac tctcgacgct tggaacgtaa gcgagcggca tcggcaatcg atacatgcca
     4561 cgaggcacgg ccactcgggg aggggaggga ttcgcgaata acgactctgc ggcctggcca
     4621 cgaatccaag gctctcaaac cgccttcctg taggcggccc cttcgtaaag tgcgcgaagc
     4681 cagccgcatc cacacgcaag cgcagcccag gcccccgact cacccgcacc accgcgctcg
     4741 ccgcgcccag caaccaagag cattccagga gcgcggagac gcgggccgag agatcgcgcg
     4801 ccccgacgct cccgctggca aaggcgttag gtgcgcttcg gcgcgctcgc gaggcgatgt
     4861 gccatatagc gcgccggcga cgaaccgacg gctttgcgaa acatcgtcac gaagctgctg
     4921 gcgctctcgt acccgagctc gaccgccacg ttctgtacgg cggcaccccg cgataacttc
     4981 tgaatcgcga gcacgatgtg gagttgctgg cgccagcgga cgaaactcag ccccgtctcc
     5041 gccaccagca gtcggtgcag cgtgcgttcg ctgactccgt tcgccgtgat tctggtcgcg
     5101 ttctctctgc gtctcgcgtt ttgctcgtcg cgaagggtcc agcaccgcgc cgcaccccca
     5161 aggtgcactg cattctcacc ggcaaagcct cctcgatcca gcgcctctac aaggccgcgc
     5221 gtcgagcggc tctgccttcc aagcaagtca ccagcgtcgc ttactgggct ccggggcgca
     5281 agggtttctc agggatccag cgctaagtaa tccaagcgct ctccgtcgcc gcctcttcgg
     5341 tagcgacgcc agcaccgggt caacggcgct ggcgcactcg tcgggcacgt tggcgctgct
     5401 gctctgctgc tctgctgccg cgctcactcg atgttagcgg atttgtagac ccagccgcta
     5461 ttcgtacaga acccgcaaga agagccatag aggaaaccct tcccggttgc gcccatatca
     5521 cccttcagct gccaattcag ccaagccaag ttcaccttgt tgaagtctcc ccgagcttgg
     5581 cccagatctc cgccgtgccc ggcactggtc ttggagaagt agacgatcgg aactccgcgc
     5641 ttgctgatct cgttgtagtc tcgcaggcca ttctcgtagg cgatatcgct cgagccgccg
     5701 ttcatgatct tgaagggtgt atgaatcttg tcgtagagcg aggcgttcgc gctcattagc
     5761 ccgctgctcg tgaacccaat ggcagagaat cggggatctc ccgagacatt ctccgccatc
     5821 aaaccgccgc aggagaatcc atccgcggcg atcttcgtgg tctcgatgct ctgatagtac
     5881 gcactgcacg acttgtcgtt ctcggcgata gcccaggtga tgtagtcgag gaacgccttc
     5941 ccattctgcc cgccttggca agcgttggag gcacccggcg taccgtctgc aatgacgaag
     6001 tacccccaag aggctatttc gctcattgcc gcttggttcg atagcccgtc ttgagaacaa
     6061 ccgccttcgc cccaaaccag aatggggtac ttctccgctc cgccgagctc cttcggcctg
     6121 tagatggtac cgcacttgat tccattctcg gagttggtct cgatcaccac cttgtgcggc
     6181 ccgctaccag acgcgctctt gcctgtgtcc ttcgatgcgg tgcaggtccc gctcgactgc
     6241 ccaccactcc cagtggcccc gccggtccct ccactcttcc cacctgagct ggtcgcccca
     6301 gctgagctca tcccgcccgc gccgccttta ccgccgctcg tggcgctgcc gccgcccatg
     6361 cccatcccac ccgagctcgc cccgcccatc cccgatcccg ctacgccagc ccctcccgtg
     6421 cccgcgccgg ccgtgttcat gacgcctcct gcggccccgc cggtgcccga gcccgcagca
     6481 ttggtgcctc cgtttgctcc accggttgct gatgacgcgc cccccgaggc cgtctgacct
     6541 ccctgcgcgg ccgttgaacc gccttgcgtt gcttgacccg gcgacgtctg cccgcccgtt
     6601 gcggccgctc ccccggtgac cttcgcgcca cccgctccac tgctcgcacc gcccgagtta
     6661 ccgggttgca tgcctggctc cgggctactg gatgagcacc caacgccgct tatcgtgaga
     6721 cccacgaagg ccaataaagc ggttcgcatg gggcgcgagg tgtttctgtt tgcttttacg
     6781 tcggcgttgg cagcagcaca gctcatgggc gtgcagcggt cgagagcacg agagaacggt
     6841 ccaaaaaaaa gctcttgcca acgccttgaa cttttctcgt ggaattgcgt cctccgtttt
     6901 gggtgagctc actcggggcc caccacgagt agggccgttc ctccgggcat gtggctcgaa
     6961 gttgttggcg caactaggtt gcaaatgacg agccgcggct ggcgctcgtc ggcgtgagtc
     7021 agtcctctac gagcggcgag ctcgttggcc atgcagtaaa tgggtcctgg agctcttgcc
     7081 agaggttcgg cccaccctcg agctccgtct cggtgagaag agccgagtcc agtcgccgcc
     7141 ggagggcccg ctcgtttatc cgggccccga tgaagaccaa ctcttgacgt cgatcgcccc
     7201 acggctcttg ccagtcgcgc tcgagttgcc ggcgggactc gtcgtcgtcg ggccagctgc
     7261 cttctggcat tgctgagtac cacatccctg caggttcgca gctggccgag ctcccggctt
     7321 gcgaccaaag tccggccact tccatgcgtg acgcgagcca gaagaaaccc ttcgatcgga
     7381 gcacatcttt ccaggtgtcc gggtcttgaa ggagtgtcca gaagcgatct gggtgaaacg
     7441 gaacgcgagc gcggtagacg aagctagaga taccgtattc gagagtctct ggcacgtgct
     7501 cgccacgtag ttccttgagc caccccggag ctcgagcggc cgcctcgaag tcgaagagat
     7561 cggtccccaa cacggcatcg agcggaactt tcccattatc cgcatgaacg attcgggcct
     7621 ctggattcaa gtgccgaagc atgctcgcga gccgctccgc atccgcggta gacacgagat
     7681 ccagcttcga gagcacgagc acattggcga actctacttg ttcgacgaga aggtctggga
     7741 gcgaccgctc gtcgtcttca ccgagcgcaa ttttccgggc gcgcaggtcc tcttcactat
     7801 gccagtcggt caggaagttc ttggcgtcga cgacggtaac cagggtatcg agccgcgcga
     7861 cttggcttag actctgaccc aattcatcct cgaacgtgaa ggtctccgcg atgggaaggg
     7921 gctcggaaat gccggtagat tccacgagca agtagtcgaa gcgtccctct ctcgccagcc
     7981 tgccgatctc gaccaacaag tcctcacgga gcgtgcaaca gatgcagccg ttctgcatct
     8041 cgacgagctt ctcgtcgacg cgtgacaggg cggctcctcc cgtcttcacc agccgagcgt
     8101 cgacgttcac ctcgctcatg tcgttgacga tcacggccac tttgcggcct tcacggttgg
     8161 cgagcacgtg gttcagaagc gtagtctttc cggcgccaag aaagccggac aataccgtaa
     8221 ccgggagcct gcgcgatgcg gaatgcatgg tgactcacat gcaacaaggt tgcatgtgag
     8281 tcaagcttga tcgagcctcc caggtgcaaa agcaactgcg ttgcaaaaaa aaaagaagaa
     8341 gccgacgaac tcgacccgcc ggcagccccg gcgatctctc tctcgtcgag ctgctcgccg
     8401 atcacacaga acccgttgcc gaacgggtca ctgcaaaacg cggtgggctt tggcccctgc
     8461 tcccggaact ccagttcgat ctggccgcct cagtctcgca ctttcgcaag cactccgtct
     8521 aagtccccaa cgtgtcgatc gaggtgtacc ggcgtccagt gcgcgccgcc aggcgcgtct
     8581 cgcctggcgg cgttggtcgt cgcgacgctc gtctgcctcg acgcagcacg gcgccgtgtc
     8641 tcgccgagca ggaagagcct tcccggccgt gctccctcga gctctactcc ttgatttccg
     8701 gctccacgag ctgcgtcagc aacgtcaggg actcttgcca accgaggtaa caagcctcga
     8761 ccggaatgat gctcggaatg ccttcctgta gaacggtgag ctccgtgccg acagagacct
     8821 tcttgagtga aatcgtcgtc tgcatctgac ccggcaagtt ggggtcgtcg aacttgtcgg
     8881 agtggcgaat gcgttcattc ggcacgagct cgaggaactc gcccccgaac gaatggctgt
     8941 gcccggtcgt cagattcgtg aaggacatct tgtacgtccc gcccacttcg gcctttatgt
     9001 ggtggactct acccgtgaag ccgtgcggcg gaagccattt ggccatggca tcgggatcga
     9061 ggaatgctcg atagatgcgc tcggccggtg cccgaagtac ccgatgaagt ttgacgctgt
     9121 tctcagccat agctcgcttc tccttggtgc tgcggcagac gccgcgattg gcagtcagct
     9181 accgcatgta caatggcgac gagcgggacc cgaccggatc gacagcgctg ccggttttat
     9241 tttcgagctg acttctgggg cgaagttgcg tctgactgac tcgacccctc cctaaaccag
     9301 catggtggtg ggctcacgtc ggtcggcccg aagttcaggt gccgggcgat ccgctccggt
     9361 aagggtgcgt ccctttccgc ttccccgagt tcagcgcgcc agcccgtgct tgacgtagct
     9421 gctgggcgtt acgccgagga gctcgcgaaa gtctctcgcg aggtgtgctt gatcgtagta
     9481 gccggcgtct gccgcaatgc ggcccaaagt gtgtgaagtg gaggtgtggc gcacggcacg
     9541 gcgtaggcgc gcgctgcgcg caaactcctt cggcccgatg cctaccgtat ccgtgaacgc
     9601 gcggcgcaaa tggcgggccg tgacgcctag ccgagccgct agggactcga tactcgtctc
     9661 ctgctcttcg agcagcagca ccgcgcgccg tgccaggcgt gccgacgccg attccgagat
     9721 aaggggcagt cgggtggcga aagcatgcgt gatgcgttca atcgccgcgt cggcactccc
     9781 agtggacaac agatcggcca acaacgagcg ggcgctgctc ccccaaatct cctcgagcga
     9841 tacgacgtgg tcggtgagtc gtctcgcacc gacccccagc atcgcggggg cccaacccgg
     9901 cttgatttga atcaccaccg cgcgctggat gccgcccgcc tccttggtgt aagcctgcgt
     9961 gcgagggcca gcgacggaca gatccccgtg gttctcggcg agcttgcgaa agacgaagag
    10021 ggtccgcccg tcgggcaggc gatcgatgcg atgccgctgc ccgggcgtgg actcgtacac
    10081 gcgaacctcc tcgacgaagc gcgctacctg catggacaca gtatggctcg gggtgcccat
    10141 gcggccaaca gggatttctg ctcatggtga gcagtgagac ctcgttcgat cgctcttcga
    10201 gcgttcacgt gtcttcgagt agcacagggt ccaagggtgg aattggacag aacggacatt
    10261 tgggttggcg ggagggcagg ggctcagcgc tcgcaggcct tgcggccata gtcgcgcaca
    10321 ccgctatcga gcagctcgaa cgctcggtcg caggttcgtc ggagcccccg cttcatcttg
    10381 gcgggggagt cgccgcggag caccgccgcg cgggcgtctt ccatcagcac ccgctcaatc
    10441 gccagaatca tggccgcaac cgcgcgcggc gtaggatcgc ctttggcgac ccgggtcgcc
    10501 tcggagatcg cttccgtgag cgccgcttcg gtcttctcga gggcgaatcg ggcgcgagcc
    10561 cggaggctgg ggctcgtctc gagcgtgcgt acgaatcgac ccccgctagc cacgaggtga
    10621 gacagtgtgt ccccgtcaat tgccgcgcgg aacgctcggt gcaacgcccc cggaattgac
    10681 tcgccgggtt cgcgttctcg aacgaagcga cggtgcgctg cgatgacttc gccttcacga
    10741 tcgaagaaca ggtcctcttt ggtctcgaag tagttgaaga tggtcgcgac cgagacttcc
    10801 gccgcggccg cgatctcggc cacggtcacg gcttcgaaac cccgctcgtt gaacagttcc
    10861 gtcgcgattc gggagatcgc gagccgagtc ttggccttct ttcgctcgcg caggccaggt
    10921 tcaacgttgg gcatgggcac cgaaatgtat cagcggggcg tctgccgaac gagcccgaaa
    10981 attgagttag acaaaactta gagtaactat aagattagct cgacgtaaag aaagcgatct
    11041 gatgcccccc actacccagc ggcgcaatcg ccaatggctc ctgaagacca ggccccacgg
    11101 tgcgctctcg ctcgacaact tcgagttccg cgaccaggag atgcccgaac cggagctcgc
    11161 gtctggccaa attctcgtaa agaacctgct gttcacctgc gcgccgacga tgcgcaactg
    11221 gctgaacgcg ccgggcaact cccaccgcac cgtgaacccg ggtaccccca tccttggccc
    11281 tgggggatca gaagtcatca aatcgaacca cccgcgcttc cccgtcggta ccagggtgac
    11341 gttgctctcg cgctgggagg actactcggt tctcgagcca gatgcctcgc ccacgccggt
    11401 cattgcgcat tcgtcggacg tgggcctgag ccaggcgatg ggcatcttgg gtctcaacag
    11461 ctgcacggcc tatttcgggc tgctcagtgt cggtcaagcc aaagcgggcg agacggtcgt
    11521 cgtttctgcg gcggcgggtt cggttggttc gatggtcgtt cagatcgcaa aaatcacggg
    11581 gtgtcacgta gttgggatcg cgggtggagg cgagaagtgc cgcctggtgg tcgacgagtt
    11641 cggcgcggac gccgcgattg actacaagca cgagaatgtc gcagtgcgac tcggcgagct
    11701 ttgtccgagg gggatcgatg tcttcttcga caatgtcggc ggagagatcc tcgatgcggt
    11761 gatggacaac attgcgttca agggtcggat cgcggtgtgc ggccaagtgt cggcctatga
    11821 ctccggcacg ct
//
LOCUS       DASTKU010001074        20590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_212894, whole genome shotgun sequence.
ACCESSION   DASTKU010001074 DASTKU010000000
VERSION     DASTKU010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 20590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20590
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212894"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VFQ61_38135"
     CDS             <1..775
                     /locus_tag="VFQ61_38135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET9960392.1"
                     /translation="GETTDKLEPFRAEGMASRVLGMGDVVGLIQEFEGVIDQKKAEED
                     AARMMSGQFTLDDFLNQVRMIQKMGSLKDLVDKIPGMGNMLPPGAAAQFDDRELVRIE
                     AIIQSMTRAERNDPHALIREPSRVRRISKGSGQPEQGVSELVQKFLFMRQMMSGMGQD
                     MGMLGNIPGLKNLAMARNLRRAAKNGQLPGMPGMGMPGMGMPGMGFPGMPGFPGMPGM
                     DAGGAEAPRMKLLSKAEKNAKKNQRKRERDARKKNKGKK"
     gene            775..2256
                     /locus_tag="VFQ61_38140"
     CDS             775..2256
                     /locus_tag="VFQ61_38140"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HET9960393.1"
                     /translation="MMSRKRSFLVLSGAVLSLVSSLTLVGCSSCSREEKKVDLTQFVM
                     DAAPGDITKLEVDFDGKVKLLGYKWNEKRGMRPGRPVDITLYWQPTQKLEDNAHLYVH
                     LLDESGERIAVLDEDSPIRELRDGKPALPPSSWQPGKVYADELSFKVPRRVKTSNVQL
                     VAGVQRGEERVKPTGGQNVDGSALIVTAGTGVKARTPQAESVAEMRVDRLPAKTKLNI
                     DGKLDEEAWKEAPSAPFSALSREQRRKKMGVDGSIHVLWDNDGMYLGFDVKDRNVTGG
                     FSTDDKSPQLWKKDAVEVILDPEAEGDSKDYYEILVNPQNLVFDTVYDDSSVPADGPY
                     GHEDWSAGVKSAVTVHGTLDNDQDQDEGYVVEMFLPWKSFSKAKKTPPVLGDTWRMNF
                     YAINERDLVGWQSYPTLRGPLQTSWFGRVTWAEKGFQIQAATAPSASSAAPAASGSAA
                     SAPAPSGAGKAANAPVKPAMAPAPVGATPAAPKAATPKADVPK"
     gene            2505..3338
                     /locus_tag="VFQ61_38145"
     CDS             2505..3338
                     /locus_tag="VFQ61_38145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960394.1"
                     /translation="MAAFSSAVDPSLRPPRTNWFFRVRVSILLGILLLVLGWAARDMQ
                     QRRARNAWEAPVRVALVVLRLGPTEPAGVHRLMLRTRALEQRLSDEFRRYHPGAPNMF
                     RFETFGPLEITHAPPSDTGNTLLERAIHAFSLWRYTTAVNTSARLDSRDFDARLYLVI
                     EPTQGGQQFVEGFSEDGGRIGVARVNLDPTTVDLSLFVAGHELLHTLGARDKYDAASG
                     FTLIPDGLAEPDRYPLYPQRGAEVMARNRVLMPGAERPPEALGELFVGRATAREIGWL
                     K"
     gene            3387..5660
                     /locus_tag="VFQ61_38150"
     CDS             3387..5660
                     /locus_tag="VFQ61_38150"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9960395.1"
                     /translation="MSTLRVQARTAVLTLPSEGTPTRRFQRVAVRLASPWTVLIGFPL
                     VTGLVSLLLNLQSQQRLSDLGRRAALERFAQRARFAERHLGQALSQADQLLDRTRELA
                     LEHASQAPYQSFAFALRDLALSRAGLKWLSVSFPDGTFEGVFLDEADRLCFQISRVEG
                     GRTSMQQFNFGPGSLVERARDEKFYDPRERTFYRNAVQAGHRIWTEPYPFLPDYRSGI
                     TRAEAVLAANSKLHAVITADYDITELSGLLESGVRLHTPTVVFTPSGSLLAVSGIHDP
                     GLTRAPSSTRPLQFRDLKSPLLRTFFETRAGQAPAVGARSFRVGDRNHVALEHRLSLG
                     PELDFRLAALLDEASLLDSAHRQAREGLFTSLAISLLGVLLSIGVAMGIQRLRLQRAN
                     ADQRATLAIQQARELGSYRLIEPLGSGGMGEVWRAEHRMLARPAAIKLMRTDLPGVDP
                     SVLEARFELEARTLSQLRSPNTVSVFDYGRTTDGRLFLVMELLEGLPLDKLVEKFGPQ
                     PVGRIIPMLIGVCRCLSEAHSAGLVHRDIKPANIFLCYDGEGVERVKVLDFGLAKVAR
                     GAGLTAEGSVAGTPDYMAPEQARGTAIDGRADLYALGCTAFFLLTGRPVFLEATALGT
                     VLAHQMTAPPALTQCTSQWIPSELELLILRCLSKDPEYRPRSAASLLRALQDIRIPDA
                     QRASAEFLREWWEAWAAQAQREPVAPRAAGPNGTLGGAADAAASAKRVVVDGAGAEDQ
                     PAPSPGPAGTRFGGRAA"
     gene            5657..6916
                     /locus_tag="VFQ61_38155"
     CDS             5657..6916
                     /locus_tag="VFQ61_38155"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9960396.1"
                     /translation="MMMSELESARLQLSRETRLNDPELTRCLHQVARVAATTLNVDRV
                     SIWVLDEDWSEIECRLLFDRKLEQFTVGARIPLTGFPKYTQALRERRVIAAENALLDP
                     RTSELGSSYLVPNGVGALLDCPVYENGNVVAVVCHESVGGPRAWSKRDADFAVSVAEL
                     LSTITAQAARLGFEAELRRARDALTQAQVMDSLGRMAAEAAHDFNNVLTGIALSLQIV
                     ADALPPQTPPTTAQLAARDALALVDQGANLVKQLLTFARQGTYAGLPTDITQAVESCL
                     PRLRRILGPDVTLTVDLERTPGPIRMDSSLFEQVLTNLLVNARDAMPGGGHLWLTSRR
                     AEQRITLSLRDTGVGMSEEVRQRIFEPFFSTKGPGGNGLGLSIVFGAVRGIGGEIRVE
                     SEPGRGSTFTLELPVEGAGSGPPLRGP"
     gene            complement(6944..7435)
                     /locus_tag="VFQ61_38160"
     CDS             complement(6944..7435)
                     /locus_tag="VFQ61_38160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960397.1"
                     /translation="MNPDRAGQTSQPFPAQRRRGTDRVGALVLCLLGFASACRSTQAR
                     HLEGEVQSLSRHIDALRDAPNEQKSELLARLEQQPAKSGRASELKALCVDAYRKHVTA
                     LTASGRARELLATKDGGPAAALEAAKQITLSESELSQARELTERCVSLQGQLRREAQD
                     RAN"
     gene            complement(7432..7968)
                     /locus_tag="VFQ61_38165"
     CDS             complement(7432..7968)
                     /locus_tag="VFQ61_38165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960398.1"
                     /translation="MVAANKIICASCGFQNTPPLANNRCVSCGAKIEDVKRQVSRQEE
                     LERRYQQEGFSLPWFGVSLAIILVMTAALVMGLPMVIPAFDFEGSAGMMIAIPVWFMG
                     GVLIGLISPGRTFIEPVLAVFLVALPTAFTLYMNQTVKTMPAFMYVLMSILGILFTLI
                     GSYLGERVQLGPPPKASE"
     gene            8162..8860
                     /locus_tag="VFQ61_38170"
     CDS             8162..8860
                     /locus_tag="VFQ61_38170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960399.1"
                     /translation="MSEPPHPARPEAMPLEPSADPAAYGSEPEASPETRDLAELPAPH
                     KPFRRITFGVMALTAILSLALASGLSGELTYSLRGGGPQDLGELEQMSDRAFRPNAWV
                     RGAGALSADSVVRYARPLERDTYRLAQVEGNPRLWVELRVPLEADGEHFVAPGSFVGR
                     LVQSEAAGLRYSALEEAARDAGHALPKDAWLLIDGESPASTRWVIGLQLLLLGFAVFN
                     VGGILRLARPVRDA"
     gene            8867..10081
                     /gene="thiO"
                     /locus_tag="VFQ61_38175"
     CDS             8867..10081
                     /gene="thiO"
                     /locus_tag="VFQ61_38175"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HET9960400.1"
                     /translation="MKRTSKPSARNTTRAIKKRNLPKPPSALAPRTAAGHATSFDVIV
                     IGGGIMGTGASFELAKRGARVLVLERSVPGAEASSAAAGILGAQAEAHHDSDWFELSL
                     KSRQRHAEWAKELLERTGIDVEYRLCGVVRASATLEAAQAMQAERAFQRRYGCRVSDL
                     DARSLSRLLPQFSSKLQGGVYFPEDARVDPPKLLRALRIAAEKAGAVFRSGTYVRRVL
                     RDTKRATGVALDDGTELKAKHVVVAAGSWTSLVEGVPTTRIVKPARGQIVELSAPAPL
                     FEPVVFGPGCYLVPRDDGRVLIGSTLEFVGYRREVTAQAVRNLLDAALTLVPALADAT
                     LSGTWSNFRPYTSDETPWVGEGEVPGLVLATGHYRNGILLAPITADLVTAAIVGDSGK
                     RRPRAARPADTE"
     gene            10175..11743
                     /locus_tag="VFQ61_38180"
     CDS             10175..11743
                     /locus_tag="VFQ61_38180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene dehydrogenase"
                     /protein_id="HET9960401.1"
                     /translation="MPSRYYDAVVLGRSLGALAAAALLSRRDFRVLVLGQGQRPQTYR
                     FEGRVLCRRSFTLLGASCPAFRKLLQELAYSPQFRRRTQNLDPMFSVLYEGRRVEVAP
                     DMELFAREIDREFPEVRQLVDELYTAVAHVNAAADLAFERDAVWPPQSLWERIETGRL
                     SYSLPFLHGDRQSDLLGKFPRGHAFRDLVYVPAKFASNLAGAAEDLPAFALARLHGAW
                     TRGIAQLAQSEDELTELLTERIQTHGGACNLTGRARSLIVKHGQIAFVQEDGADEPTG
                     TGVVITDQYGEALADLTGGAGITKSARRDWPRLAPEAGRFVVSLVVKKGLLQAPHGQV
                     TFVLPDSRVRKDPRRPIVKFERLDPAAFPDVAAEARDEALLVAEMLLPYRGALTLLEA
                     REAVLSTLRGALPFLDEHLICVDSPHDGLPLYDYRGGVRRDVERVHLEGASSNPETME
                     RIWSVTPTGYLGLSAEPLRGPVPGTFLVGKTVLPALGQEGELMAAASVARIVTRRDST
                     RQRMRRQMWSKIET"
     gene            11751..12326
                     /locus_tag="VFQ61_38185"
     CDS             11751..12326
                     /locus_tag="VFQ61_38185"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HET9960402.1"
                     /translation="MGVRVIAGEHRGRILKTPAGRSTRPTGARAREAIFSILGPLGGE
                     RVLDLYAGSGAFGIEALSRGADLSVFVEAEPRALACIRQNLSELGLQERARVYPVRVE
                     RAAPALQAHLPFTLVFCDPPWADWVFAADAVAKLSGLLATGARLVFEHPAVDYPGEHN
                     GLREAGLRELDQRRWGDTGMTLFERVDASHS"
     gene            12426..13022
                     /locus_tag="VFQ61_38190"
     CDS             12426..13022
                     /locus_tag="VFQ61_38190"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9960403.1"
                     /translation="MTSTTPPPPKTGGSAPFIIAAIVMLLLMGGLIYWKTRGDDGPTS
                     KVTQPAAPATTPAAPIFEEPPPPPPPPEEEPKDAGVDNKAPKRALGSGPGGCNGECKG
                     EATSAMRSMLAAKAGQARGCYERALRQNSMLQGRLKIGVRISSTGSVCSANVVQNELG
                     DAGIASCVAQIFRSSTFPAPTGGSCVDAEVPLRFEPKQ"
     gene            13022..13321
                     /locus_tag="VFQ61_38195"
     CDS             13022..13321
                     /locus_tag="VFQ61_38195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960404.1"
                     /translation="MARKRLLLACGLGVYALAACSLEGTTPSCPNLPEYDVRDAQARQ
                     AAWPALLAASEQGCITLPDDAEAAGGASGGATAVTGAGSSGLAGESAGGAAGTAP"
     gene            13430..14338
                     /gene="truA"
                     /locus_tag="VFQ61_38200"
     CDS             13430..14338
                     /gene="truA"
                     /locus_tag="VFQ61_38200"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET9960405.1"
                     /translation="MNQDQTADAARTANAALASTEGDRAANTPEPHSSRSVLLTVAYD
                     GSNFAGYAAQTNARTVAGELLGAIRALDPKVKSLRGTSRTDAGVHARGQLVAFDTGTR
                     IPARGWVLGLGPHLPKEIAIVRAALVPEGFEPRRHVVRKTYRYVLLRSAVRDPFLESR
                     AWRITDRLNHTDMQRAADALLGEHDFAAFRGAADERENTVRRMFRVEVRTARSDERIL
                     EIVVEGDRFLYRMVRIIAGTLVDVGRGRLDPGVIGRAIASKRREDLGITAPPDGLFLD
                     RIELDEAPGECFPPREPGKASDQSSD"
     gene            14655..16208
                     /locus_tag="VFQ61_38205"
     CDS             14655..16208
                     /locus_tag="VFQ61_38205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET9960406.1"
                     /translation="MLNPSVMRWVTDDRVMKATEGLLDARTRVKAAVQVLLNGHELPP
                     VDPALDPSIAGIETGRGSEAKNGNSNGHSVANGNGNGKASASISISDVSTRSARGSED
                     MDESLRERSTLASIGGRDVLEKCQKFMAADNARKMGIYPFFRPLDLNDGPEAVINGRR
                     VVMFGSNNYLGLTTHPRVREASKAAIDRFGTSMTGSRLVNGSMKLHEEFEEKLAAWFG
                     KESALVFTTGYQVNVATCSALLSNKQSVAVIDRNVHASLYDGVRLGQSAGARLVRYRH
                     NDAESLDKTLEKLEAGEGALVITDGVFSAEGEIAHIDQLVPIVKKHGARLFVDDAHAL
                     GVIGPEGRGSSHHFGVSAEVDLIGGTFSKSLASIGGYLVGERKVLDYIRHFAPSFMFA
                     AAAAPPCVAAAMAAFDVMREESWRIDKLHENFNYMRTELQKMGFELGHTQTAVIPIFV
                     RQDLRTIMMWRDLLEEYGVYTNPFISPGVPPKSAMLRTSYMATHERSHLDRGLEAFEK
                     VGKKYGVIK"
     gene            16518..16880
                     /locus_tag="VFQ61_38210"
     CDS             16518..16880
                     /locus_tag="VFQ61_38210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960407.1"
                     /translation="MKKPSDIQELADALGGLAVFGVIPGSPVALCGVRYGDVVLTTNG
                     YTTRTLDEFFRARATCESHLELEVFREGRLLRLNAPLSSSRELSLDQVSSSVIDGEML
                     PTASSRRQASVVESRFRS"
     gene            17010..18515
                     /locus_tag="VFQ61_38215"
     CDS             17010..18515
                     /locus_tag="VFQ61_38215"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9960408.1"
                     /translation="MEEAQVVLIVDDDVVLANSLSRALSGRGLTVLVAHDRHSALGVL
                     GRERVHAVILDLQTPGADGLELLERMRDRRADRPRPVILLLSGHLDVRTTVRAVRAGA
                     FDVLEKPISDAVIYERLRAGLQQGSNSTSPPSTPISPPESTGSPKDRDAADRLLGKNA
                     AVRAVREQIRNVARFPDLSVVIIGETGTGKELVAEAIHILTGSRGPFVPVNCAAIPEA
                     LFESQLFGHEAFSFTGARGTHTGLLGEAHGGTIFFDELGEMPPQLQPKLLRVLETRSY
                     RRIGGTRDLPLQARVISATNRRLTGREGVVRPDLYFRLAGFTILMPALRERTSDIDSL
                     ATHFIDEFARRYPEAPNRLTERALEALHAYDWPGNLRELKAVVQQAAVITSARSVGVA
                     EIARVLDERNARPASAPSASLRLAPTSAPSADPEPGSSPRASSPEGPLASPPHENHAA
                     ERRPRSGEHRIERTAIQDAFLASGRNLSATARSLGLPRTTLRDRLRKYGLL"
     gene            complement(18614..19285)
                     /locus_tag="VFQ61_38220"
     CDS             complement(18614..19285)
                     /locus_tag="VFQ61_38220"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET9960409.1"
                     /translation="MTSVLIADDDEALGAALLRDLGRRGLFVAWARSEEEARRLLKAE
                     WFDVVLLGLPIADVERIIGGGDSKNRRPYWVLCCSDTGDGVRKRAVAVGIWHVLRKPF
                     GPQHVLAVLAHTATGVAPGERRSLIDQMQAAHRSRQSIELEFVGETVGRVVLCDGEIV
                     HAAFGGLHGEDALQAILDTRPTPLVSPISEAKERSIRRPFQTVLFDALRKLDEREQPA
                     MGRNG"
     gene            complement(19282..20331)
                     /locus_tag="VFQ61_38225"
     CDS             complement(19282..20331)
                     /locus_tag="VFQ61_38225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9960410.1"
                     /translation="MSSNARIDLESWLELHDAPVLLCDDLGRVCMATPEALALLPELE
                     RAANSSEAVLPMAAIRAIREQPFGTPWLWPTSETGLGISCVAFEAGADRVLLLFRTDA
                     SHASVERRLHRQRLELTGQLTASVAHDLRSAVASIVYNSDALIAEDRGAEESVPPELL
                     RETLLDLRAASARLQFAVDGLLHFVRGGPRVCLDVRLEEVFGRARNFVRWVYRERGHS
                     LEFSIAEGAESVRGSPVLIEQILVNLLLNAAEAASTGLHVTVSAKPPPAGEGHVRIRV
                     ADDGPGIPEEIRERIFLPFFSTKRSGSGLGLSTARSAARELEGQLELVPRERGACFEI
                     TLPKVMATKTLEGVE"
     gene            complement(20389..>20590)
                     /locus_tag="VFQ61_38230"
     CDS             complement(20389..>20590)
                     /locus_tag="VFQ61_38230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960411.1"
                     /translation="ARQVREIAYGTLRIVYLSTGLQHDGFGVSRHVGLSADPIRAGGE
                     HVISGVAHLAICWLCLDIQIVA"
BASE COUNT         3800 a         6564 c         6593 g         3633 t
ORIGIN      
        1 gggtgagacc accgacaagc tggagccgtt tcgcgccgaa ggcatggcga gtcgcgtgct
       61 cggcatgggt gacgtggtcg gcctcatcca ggagttcgag ggcgtcatcg accagaagaa
      121 ggccgaagaa gacgccgccc gtatgatgtc ggggcagttc accctcgacg actttctgaa
      181 tcaggtccgc atgattcaga agatgggctc gctgaaggat ctcgtcgaca agatcccggg
      241 catgggaaac atgctcccgc ccggtgcggc ggcccagttc gacgatcgcg agctcgtgcg
      301 tatcgaggcc atcattcagt cgatgacccg ggccgagcgt aacgatccgc acgcgttgat
      361 ccgggaaccc tcccgggtgc gccgcatctc gaagggctcg ggccaacccg agcaaggcgt
      421 gtccgagctg gttcagaagt tcttgttcat gcgacagatg atgtcgggca tggggcagga
      481 catgggcatg ctggggaaca tcccaggctt gaagaacctg gccatggctc gcaacctgcg
      541 acgtgccgcg aagaacggac aactcccggg tatgccgggc atgggcatgc ccggtatggg
      601 tatgccgggt atgggttttc caggaatgcc gggctttcca ggcatgcctg gaatggacgc
      661 gggtggcgcg gaggctccgc gcatgaagct gctgagcaag gcggagaaga acgccaaaaa
      721 gaatcagcgg aagcgcgaac gcgacgcccg taagaagaac aaggggaaga agtgatgatg
      781 tcccgaaaac gttcgtttct cgtcttgagc ggagccgtgc tttcgctcgt ctccagcctg
      841 acgctcgttg gttgttcgag ctgcagtcgc gaagagaaga aggtcgatct cacgcagttc
      901 gtgatggacg ccgccccggg cgacatcacc aagctggagg tcgacttcga cggcaaggtc
      961 aagctcctcg gctacaagtg gaacgaaaaa cgcggcatgc gcccgggacg ccccgtggac
     1021 atcacgctct actggcaacc gacacagaaa ctcgaagaca atgctcacct gtacgtccac
     1081 ctgctggatg agtcgggtga acgcatcgcg gtgctcgacg aagacagtcc gattcgagag
     1141 ctgcgagacg gcaagcctgc ccttccgccc tcgtcttggc aacccggcaa agtgtacgcg
     1201 gacgaactca gcttcaaagt gccccggcgc gtgaagacct cgaatgtcca actcgtcgcc
     1261 ggagttcagc gcggggagga acgggtgaaa cccacgggag ggcagaacgt ggatggctcc
     1321 gcgctgatcg tgaccgccgg cacgggcgtc aaggcgcgca cgcctcaagc cgaaagtgtc
     1381 gccgagatgc gagtggatcg actgcccgcg aagaccaagc tgaacatcga cggtaagctc
     1441 gacgaagagg cctggaaaga ggctccgagc gcgcccttct cggcgttgtc gcgcgagcag
     1501 cgacggaaaa agatgggcgt cgacggcagc attcacgtgc tgtgggacaa cgatggcatg
     1561 tacctgggct tcgacgtaaa agaccgcaac gtgacgggtg gtttctccac cgacgacaag
     1621 tctccccagc tttggaagaa agacgccgtc gaggtgattc tggatcccga agccgaaggc
     1681 gacagcaagg attactacga gatcctcgtc aacccgcaga acctggtgtt cgatacggta
     1741 tacgacgaca gctccgttcc cgccgatggg ccctacgggc acgaagactg gtccgcaggg
     1801 gtgaagagcg cggtcaccgt gcacggcacg ctcgacaacg accaagacca ggacgagggc
     1861 tacgtcgtgg agatgttcct gccttggaag tcgttcagca aggccaagaa gactccgccg
     1921 gtgctgggtg atacgtggcg tatgaacttc tacgcaatca acgagcgaga cctcgtgggt
     1981 tggcagtcgt acccgacact ccggggcccg ttgcagacgt cctggtttgg gcgagtaact
     2041 tgggcggaga agggcttcca aatccaagcc gcgacggcgc ccagcgcgtc ttcggccgct
     2101 cccgccgcga gcggctccgc tgctagcgcc cccgcaccgt ctggcgcagg gaaggccgcc
     2161 aacgcaccgg tcaaacccgc aatggccccg gcccccgtag gcgcgacacc tgctgccccc
     2221 aaagccgcga ctccgaaagc ggatgtccca aagtaagagc caccactcga gctcgaagcc
     2281 agctgggtaa ttcataaccc actcgggtta ttcgatggaa aacagcgccg ctttcgcaag
     2341 aagtggcgct gtttcttttt gggcccgccc ctaatccgag ctcggtagtc catcgatctc
     2401 ccaatatgag attgatgtaa ctcccttcac cacaaactcg aatttagtaa gcggaggtag
     2461 ggcggagtct ggaatttctg ccatgttgtc ctgaatcgcg gaccatggcg gcattctcta
     2521 gcgcggtcga tccgagtctt cgcccgcctc ggaccaattg gttcttccga gttcgggtca
     2581 gcatcttgct cggcattctt ttgctggtcc taggctgggc ggcgcgagac atgcaacaac
     2641 gccgcgcgcg taatgcttgg gaggctccgg tacgtgtcgc gttggtggta ttgagattgg
     2701 ggccaaccga gcccgcgggt gtgcatcgct tgatgctccg cactcgagca ttggaacagc
     2761 gtctatcgga cgaatttcgt cgctatcacc ccggtgcgcc gaacatgttc cgattcgaaa
     2821 ccttcgggcc actcgagatt acgcacgccc ccccgagcga caccggtaac acgctgctcg
     2881 agcgcgcaat ccacgctttt tctctttggc gttacacgac cgcggtcaat acgtcggctc
     2941 gcctcgatag ccgcgacttc gacgcacgcc tgtacttggt gattgaaccg actcagggcg
     3001 gccaacagtt cgtggaaggc ttcagcgagg acgggggccg catcggtgta gcgcgtgtga
     3061 atctggaccc gacgacggtg gacttgagct tgttcgtcgc gggccatgag ctcctgcaca
     3121 cactgggcgc acgagacaag tacgatgcag cttcgggctt cacgctgatc ccggacggtc
     3181 tcgccgagcc cgaccgttac cctctctacc ctcaacgcgg cgccgaagtg atggcgcgta
     3241 accgtgtttt gatgcccggc gccgaacggc ctcccgaagc attgggagag ctcttcgtag
     3301 gccgcgccac ggctcgggaa atcggctggc tcaagtaggc ttcgtcaggg ccgcgattcg
     3361 ggagtagcat gcaggcctgg taggccatga gtactctgcg cgtgcaggcc aggacggccg
     3421 tcctaaccct tccgagcgaa ggcacgccca cgcgccgttt ccaaagagtg gcggtgcggc
     3481 tggcgtcccc ttggacggta ctgatcggct ttccactagt cacgggtcta gtatccctgc
     3541 tgctcaacct gcagagccaa caacggctct cggatctcgg ccgccgcgca gccttggagc
     3601 gcttcgcgca acgcgcgcgt ttcgcggaac ggcacctcgg gcaggcgctc agtcaagcgg
     3661 accagttgtt ggacagaact cgcgagttgg cgctcgagca cgcgagccaa gctccgtacc
     3721 aaagcttcgc tttcgcgctg cgcgacctag ctttgtcgcg cgctgggctg aagtggctga
     3781 gtgtcagctt ccccgacggc accttcgaag gggtcttcct cgacgaagcg gaccgtctgt
     3841 gtttccaaat cagccgggtc gagggtgggc gcacctcgat gcagcaattc aatttcggtc
     3901 ccggaagctt ggtggagcgt gctcgagacg agaagttcta cgacccgcgt gagcgcacct
     3961 tctaccggaa tgcggtgcaa gccggtcaca ggatttggac cgagccctac ccgtttttgc
     4021 ctgactatcg gagcgggatc acgcgcgcgg aggcggtgct cgccgccaac tccaaactgc
     4081 acgcagtcat caccgcagac tacgacatca cggagctctc cggcttactg gagagcggcg
     4141 tgcgtttgca cacgccaacc gtggtcttca cgccgagtgg ctcgttgttg gccgtttccg
     4201 gcattcatga cccgggcctg acccgcgctc cttcctccac gcggccgctg cagttccgcg
     4261 atttgaagtc gccgctgcta cgtacgttct tcgagactcg cgcggggcaa gcgcccgcgg
     4321 tcggtgcgcg ctcttttcgc gtgggcgatc gcaaccacgt cgcgctcgaa caccgcctat
     4381 cgctcggacc cgagctcgat ttccggctcg cggcgctgct cgacgaagcc tcgctgctcg
     4441 attcggcgca ccggcaagcg cgcgaggggc tgttcaccag cttggccatc tcgctcttgg
     4501 gcgtgctgtt gtcgatcggg gtcgcgatgg ggattcaacg tcttcgctta cagcgggcga
     4561 acgccgatca acgcgcgacg cttgccatcc agcaggcgcg cgagctaggc agctatcgcc
     4621 tgatcgagcc gctcggcagc ggaggcatgg gcgaagtctg gcgcgcggag caccgtatgt
     4681 tggctcgccc cgcggccatc aagctaatgc gcaccgactt gcccggcgtc gatccgagcg
     4741 tgctcgaggc gcgcttcgag ctcgaggccc ggaccctgtc ccagctgcgc tcgcccaaca
     4801 ccgtttccgt gttcgactac ggccgcacca ccgatggtcg tctattcctc gtgatggagc
     4861 tgttggaggg gctaccgctc gacaagttgg tcgagaagtt cggaccgcag ccggtcggac
     4921 gcatcatccc gatgctgatc ggcgtttgcc gctgcctgtc tgaagcccac agcgcggggc
     4981 tcgtacatcg cgacatcaag ccggccaata ttttcttgtg ctacgacggc gaaggcgtgg
     5041 aaagggtaaa agtgctggat ttcggcctcg cgaaggtcgc gcgcggcgcc gggctcacgg
     5101 cggagggttc ggtcgcgggc acgccagatt acatggcgcc cgagcaagcc cgcggcaccg
     5161 cgatcgatgg gcgcgcggac ctgtatgcgc tcggttgcac ggcgttcttc ttgttgaccg
     5221 ggcgccccgt atttctggag gcgaccgcgc tcggcaccgt gctcgcacat cagatgacgg
     5281 cgccacccgc gctcacgcaa tgcacgagtc aatggattcc gagcgaactc gagctcttga
     5341 tcttgcgctg tctgtcgaaa gaccccgaat atcggccgcg ctccgcggcg agtttgctcc
     5401 gcgccctaca agatattcgg attccagacg cgcagcgcgc ttcggccgag ttcctgcgcg
     5461 agtggtggga ggcgtgggct gcgcaagccc agcgcgagcc cgtggctccg cgcgcagcgg
     5521 ggccgaacgg cacgctcggc ggggccgcgg acgctgccgc atcggcaaaa cgcgtcgtag
     5581 tggacggcgc gggcgcggaa gatcaacctg cgccgagtcc cgggccagcc ggaactcgtt
     5641 ttggaggacg cgctgcatga tgatgagcga gctcgaatcc gcacggttac agttgtcacg
     5701 cgagacccgg ctgaatgatc ccgagctcac gcgctgcttg caccaagtgg cgcgggtcgc
     5761 cgcaactacc ttgaacgtcg atcgcgtatc gatctgggtt ttggacgagg attggagcga
     5821 gatcgagtgc cggctgctgt tcgaccgcaa gctcgagcaa tttacggtcg gggcgcgcat
     5881 accgctcacc ggctttccga agtacacgca ggccttacgt gaacggcgcg tgatcgcagc
     5941 cgagaatgcg ctgctggatc cccggacgtc ggagctcggt tcgagctatc tcgtcccgaa
     6001 cggagtgggc gcgctgctcg actgccccgt gtacgagaat ggcaacgtcg tggccgtggt
     6061 ttgccacgag agcgtcggcg gaccgcgcgc atggagtaaa cgagatgcgg atttcgccgt
     6121 cagcgtggcc gagctgctct ccacgatcac ggcccaagcg gcgcggcttg gcttcgaagc
     6181 agaactacgc cgggcgcgag acgctctgac gcaagcccaa gtcatggatt cactcggacg
     6241 catggcggcg gaggcagccc acgacttcaa caacgtgcta accgggatcg cgctctcgct
     6301 tcagatcgtg gccgacgcct tgcctccgca gacgccaccc accacggccc aattggcggc
     6361 gcgggacgcg ctggcgttgg tcgatcaggg ggccaatctc gtgaagcagc tcctgacgtt
     6421 cgcccgccag gggacgtacg cgggcctgcc gacggatatc acgcaagccg tggagtcgtg
     6481 tttgcctcga ctacgccgga tcttgggccc cgacgtgact ttgaccgtgg acttggagcg
     6541 aacgccaggg ccgatccgta tggatagctc gttgttcgag caggtcctca cgaatctgct
     6601 cgtcaacgcg cgcgatgcca tgcccggggg tggtcacctt tggctcacct cccgccgcgc
     6661 cgagcagcgc attaccttga gcttgcggga cacgggcgtg ggaatgagtg aagaggtgcg
     6721 tcaacgcatc ttcgagccat ttttcagcac caagggtccc ggtggtaatg ggctcggctt
     6781 gtcgatcgtc ttcggcgccg tccgcggcat cggcggtgag atccgagtcg agagcgaacc
     6841 aggccgcggc tcgacgttca ccttggagtt gccagtcgaa ggcgccggga gcgggccccc
     6901 tctgcgcggg ccctgaggct ccgctctgca cggctcaggc tgctcagtta gcgcggtcct
     6961 gcgcttcgcg tctcagctgc ccttgcagac tgacgcagcg ttcggtgagc tcacgagcct
     7021 gcgaaagctc ggactcagag agcgtgatct gtttggcggc ttccagtgcc gcggcgggcc
     7081 caccgtcctt cgtcgccaaa agttctcgcg cccgtccgct cgcggtcaac gccgtcacgt
     7141 gcttgcgata ggcatcgacg cacaacgctt tgagctcact ggcccgtccg ctctttgcgg
     7201 gctgctgttc gagtcgagcc aacaactccg atttttgttc gttcggagca tcccgcagcg
     7261 catcgatgtg gcgcgagagg ctctgcacct cgccctccaa gtgacgcgct tgggtggaac
     7321 ggcacgcgct cgcgaaaccg agcaagcaca aaaccaaagc accgacgcga tcggttccgc
     7381 gtcggcgctg tgcgggaaat ggctgggatg tttgccctgc tcggtcaggg ttcactcgga
     7441 agccttcggt ggcgggccga gctgcacccg ctcgcccaag tacgatccaa tcaacgtaaa
     7501 caaaatcccg agtatcgaca tcagcacgta catgaacgcc ggcatggtct tgacggtttg
     7561 attcatgtac agcgtgaaag ccgtcggcag cgcaaccagg aacaccgcca aaactggctc
     7621 gatgaaggtg cggccgggcg agatgagccc aatcagaacg ccgcccatga accagaccgg
     7681 aatcgcgatc atcattcccg ccgagccttc gaaatcgaaa gcgggaataa ccatcggcag
     7741 ccccatcacg agcgctgccg tcatcaccaa gatgatcgcg agcgacacgc cgaaccaggg
     7801 caagctgaaa ccctcctgtt ggtagcgacg ctcgagctcc tcctggcgcg acacctgccg
     7861 cttgacgtcc tcgatcttgg cgccgcaaga gacgcagcga ttgttggcga ggggtggagt
     7921 gttttgaaaa ccgcaggaag cacagatgat tttattggcg gccaccatgg ttcgacctcg
     7981 gcgcgagact ttgatactag ccggcctcgt gcgcggagca caggaacaag acccatcggc
     8041 caatgaacca aatcggctat ccggtactcg cgcgggaact ctcgctcgtc tggtgctgtc
     8101 gtagccaaac aacctggaac tgccgattgg gcttttgcga tgtgtgaact tggagactgc
     8161 gatgtctgaa ccgccccacc ccgctcgtcc ggaagccatg ccattggagc cgagcgcaga
     8221 ccccgcggcg tacggaagcg agccggaagc gagcccagaa acgcgggatc ttgcggaatt
     8281 acccgcaccc cataagccgt ttcggcgaat cactttcggc gtcatggctc tcaccgccat
     8341 cctgtccctg gcgttggcga gcgggctgtc gggggaactc acgtactcgc tgcgcggagg
     8401 tgggccacag gacctcggtg aactcgagca gatgtcggat cgagcctttc gtccgaatgc
     8461 ttgggtgcga ggggcgggcg cattgagcgc ggattcggtg gtgcgctacg ccaggccgct
     8521 cgaacgtgac acctatcgcc tcgcgcaagt cgagggcaac ccgcggctct gggtggagct
     8581 gcgggtgccg ctcgaggcag acggagagca cttcgtcgcg cccggctcgt tcgtaggacg
     8641 gctcgtacaa tccgaggcag cggggctgcg ctactcggcc ttggaggagg ccgcgcgcga
     8701 tgccggacac gcgttgccga aagatgcgtg gctattgatt gacggagagt caccggccag
     8761 cacgcgctgg gtgatcggac tccagctgct tttgctggga tttgcagtgt tcaacgtcgg
     8821 gggtatcttg cgcctcgctc gccccgtccg cgacgcctga acacccatga agcgcacttc
     8881 gaagccgtcc gctcgcaaca ccacccgcgc catcaagaag aggaatctac ccaagccgcc
     8941 gagcgccttg gcaccgagaa cggcggcggg ccacgcgacg agcttcgatg tgatcgtcat
     9001 tggcggcggc atcatgggga ccggcgcgag cttcgaactg gcaaagcggg gtgcgcgggt
     9061 tttggtgctc gagcgcagtg tccccggcgc ggaagcctcc agcgcggccg ccggcatttt
     9121 gggggcgcag gccgaggcgc accatgactc cgactggttc gagctatcgc tgaagagccg
     9181 ccagcgccac gccgaatggg cgaaggagtt gctcgagcgc accggcatcg acgtcgaata
     9241 tcgcttgtgt ggggtcgtgc gtgcgagcgc gaccctcgag gccgcccaag ccatgcaagc
     9301 cgagcgagct ttccaacgcc gctacggctg ccgggtgagt gatctggacg cccggagcct
     9361 gtcgcgcttg ctgccccaat tcagctccaa gctgcagggc ggagtctact ttccggaaga
     9421 cgcgcgagtg gatccgccca agctcttacg cgcgctgcgc attgcggccg aaaaggcggg
     9481 cgccgtgttt agaagcggca catacgtgcg gcgcgtgctg cgcgacacga agcgcgccac
     9541 cggagtggcg ctggacgatg gcacggagtt gaaggccaag cacgtggtcg tagccgcggg
     9601 cagctggacc tcgctcgtcg agggcgtgcc gacgacccgc atcgtgaagc ccgcgcgggg
     9661 ccagatcgtg gagctgagcg cgccggcgcc gctgttcgaa ccggtggtat tcggacccgg
     9721 ttgctatctc gtgccgcgcg acgatggcag agtcttgatc ggctccaccc tggagttcgt
     9781 gggctatcga cgcgaagtca cggcgcaagc cgtccgcaac ttgctcgacg ccgcgctgac
     9841 cttggttccc gcgctggcag acgcgacgtt gagcgggacc tggtccaact tcagacctta
     9901 cacgagcgac gaaacgcctt gggtcggaga aggagaagtg ccaggcctgg tgctcgcaac
     9961 cgggcactat cggaatggga ttctgctcgc tccaatcacc gctgatttgg tgaccgcggc
    10021 catcgtcggg gattcgggga agcggcgccc acgcgcggct cgcccggccg acaccgagta
    10081 gcggccccgc tcgcgcgtcc gggggtggcc gtcgttgtcg ttctcgagta tgacgccggg
    10141 cagttgtacg cctgagcttt tcgaagagga atctttgccg tcccgttact acgatgccgt
    10201 ggtgctcggg cgttcgctcg gcgccttggc cgcagcggcg ctgctctccc gacgtgattt
    10261 ccgcgttctg gtgctcgggc aagggcagcg gcctcagacg taccgcttcg aggggcgggt
    10321 cctgtgccga cgcagtttca cgctgctcgg tgcgtcgtgc cccgcgttcc gaaagctgct
    10381 gcaagagctc gcgtatagcc cgcaattccg acggcggacg cagaatttgg atcccatgtt
    10441 cagcgtgctc tacgaggggc gccgagtcga ggtcgcgccg gacatggagc tcttcgcgcg
    10501 cgagatcgat cgggagtttc ccgaggtccg acagctcgtc gacgagctgt atacggcggt
    10561 cgcgcacgtc aacgcggcgg cggatctggc gttcgagcgt gacgcagtct ggccgcctca
    10621 gagtctctgg gagcgcatcg agacaggaag gctctcctat tcactgccct tcctgcacgg
    10681 tgatcgacag agcgacctac tcggtaaatt cccgcgcggg cacgcattcc gtgatctggt
    10741 ctacgtgccc gccaagtttg cctcgaacct ggccggcgcg gccgaggatc tgcccgcgtt
    10801 cgccttggcc cggctgcatg gggcttggac gcgcggcatc gcccagcttg cgcagagtga
    10861 agacgagctc accgagctct tgaccgaacg gatccaaact cacggcggcg cctgcaacct
    10921 aactggtaga gcccgctcgt tgatcgtcaa gcacggtcag attgccttcg tacaagagga
    10981 cggcgcggat gaacccacgg gcaccggcgt ggtcatcacc gaccaatacg gagaagcgct
    11041 ggccgatctc acgggcggag ctggaatcac caagtcggct cggcgcgatt ggccgaggct
    11101 cgcgcccgaa gccgggcgct tcgtggtcag cttggtcgtc aaaaagggtc tgttgcaagc
    11161 gccgcacggg caagtcacct tcgtcttgcc ggattcgcgt gtgcgcaagg atccgcggcg
    11221 accgatcgtg aagttcgaac gactcgatcc ggccgcgttc ccggacgtcg cggcggaagc
    11281 gcgcgacgaa gctttgctcg tcgccgagat gctgctgccg tatcgtggtg ccctcacctt
    11341 gctggaagcc cgcgaagcgg tcttgagcac gctgcgcggc gccctaccgt tcttggacga
    11401 gcacctgatt tgcgtggact ctccgcacga cggcctaccg ctctatgact atcgaggggg
    11461 agtgcgacgc gacgtggagc gcgtgcacct ggaaggtgcg agcagcaacc cggagacgat
    11521 ggagcggatc tggagcgtaa cacccacggg ttatcttgga ctgtcggcgg aaccgttgcg
    11581 tggccccgtc cccggtactt tcttggttgg caagacggta ctccctgcgc tcgggcaaga
    11641 gggggagttg atggccgcag ccagcgtcgc gcgcatcgtc acgcggcgcg acagcacgcg
    11701 ccagcgcatg cggcgtcaaa tgtggagcaa gatagagacg tgaggctcgc atgggcgtac
    11761 gcgtgatcgc gggagaacat cgaggacgca tcttgaagac cccggcgggg cgcagcacgc
    11821 ggcccaccgg ggctcgcgcg cgagaggcga ttttctcgat tttggggccg ctcggcggag
    11881 agcgtgtgct ggatctttat gcgggtagcg gtgctttcgg gatcgaagca ctttctcgtg
    11941 gcgcagatct gtcggtgttc gtcgaagccg aaccgcgcgc gttggcctgc attcgccaaa
    12001 atctgagcga gctcggattg caggagcggg cgcgtgtcta ccctgtgcgc gtagaacgcg
    12061 cggccccggc cctccaggcg cacctgcctt tcacactggt tttttgcgat ccgccgtggg
    12121 ccgactgggt tttcgccgca gacgctgtgg ccaaactcag cggattgctt gcaaccggag
    12181 cgcgattggt gttcgaacat cccgcagtgg attaccctgg cgagcacaac ggcctgcgcg
    12241 aagcgggcct gcgcgaactc gatcagcgac gctggggaga tacaggaatg acgttattcg
    12301 agcgagtcga tgcgtcgcat agttgaccga acggccgaga agaacagcga gcacgcaact
    12361 ttgctcgccc tccgtccgtt gggtggctat agtcccggcc cgaaggaagc ctccggagag
    12421 cttgaatgac cagtacgacc ccgcccccgc caaagaccgg cgggagcgct ccgtttatca
    12481 tcgcagccat cgtgatgctg ttgctcatgg gcgggctcat ctactggaag acacggggcg
    12541 acgacggtcc gacctccaag gtcacgcaac ccgcagctcc cgctaccacc cccgcggctc
    12601 ccatcttcga agagccacct ccgcctcctc caccgcccga agaggaaccc aaggacgcgg
    12661 gtgtcgacaa caaagcgccg aagcgcgctc tgggcagtgg ccctggtgga tgcaacggcg
    12721 aatgcaaagg cgaggcgacc tccgcgatgc gctccatgct cgccgccaag gcgggccaag
    12781 ctcgtggctg ctacgagcga gcgctgcgcc aaaatagcat gctgcaaggt cgattgaaga
    12841 tcggcgtccg catctcgtcg acgggcagcg tctgttcggc caacgtggtg cagaatgaac
    12901 tcggcgacgc cggtatcgcc agctgtgtcg cgcaaatttt ccggagctcc accttccccg
    12961 cgcctaccgg tggaagctgc gtggacgccg aggttccgct tcgcttcgaa cccaagcagt
    13021 gatggcccgg aagcgtttgc ttctagcgtg cgggctcggc gtgtacgctc tcgctgcgtg
    13081 ctcactggag ggcaccaccc cctcgtgccc gaacctgccc gaatacgacg tgcgcgatgc
    13141 gcaagcacgt caggccgcgt ggcccgcgtt gctggctgct agcgagcagg gctgtatcac
    13201 tctaccggat gacgcggaag cggccggagg cgcaagcggt ggagctactg ctgtgactgg
    13261 cgccggctca tcgggcttag ccggcgagag cgcgggcgga gcagcgggca cagcgccgtg
    13321 aagcaggcaa acacgagtgc acaccgtacc gcgtcgcgat ccatacgagc cgtgaatcgc
    13381 tcgagagcgc tccaatcgag cgctcgcggc tgacgaacga agagttcacg tgaaccagga
    13441 tcagacagcg gatgcagctc gaaccgcaaa cgcggcactc gcttcgactg aaggcgatcg
    13501 cgctgcaaac acccccgagc cgcactcgtc ccgctcggta ctgctgacgg ttgcgtacga
    13561 cggcagcaac ttcgcaggtt acgcggcgca gaccaacgcc cgcaccgttg cgggtgagct
    13621 gctcggcgcg atccgcgcgt tggatcccaa ggtcaaatcc ctgcgcggga cgagccgcac
    13681 cgatgcgggc gtgcatgcgc gcggccagct cgtcgcgttc gatacgggaa ctcgaatccc
    13741 cgcgcgcggt tgggtgctcg gactgggccc tcacctgcct aaagaaatcg cgatcgtacg
    13801 cgcggcgctc gtgcccgaag gcttcgaacc gcggcgacac gtcgtccgaa aaacctatcg
    13861 atacgtcctg ctcaggagtg cggtccgtga tccattcttg gaatcacgcg cttggcgaat
    13921 cacagacagg ttgaaccaca ccgatatgca gagagctgcg gacgctttgc tgggagagca
    13981 tgattttgcg gccttccgtg gcgcggccga cgaacgagag aacacggtgc gtcggatgtt
    14041 tcgcgtcgaa gtgcgaacag cgcgtagcga tgagaggatt ctcgagatcg tcgtcgaagg
    14101 cgatcgcttc ttgtaccgca tggtgcgcat catcgcggga acgctggttg acgtgggccg
    14161 gggtcgcttg gatccagggg tcatcggacg ggccatcgcc tcgaaacgcc gggaagatct
    14221 aggcattacg gcacctcccg atggcttgtt cctcgatcga atcgagctcg acgaagcccc
    14281 cggtgaatgc tttcctccgc gcgagccggg gaaggcaagc gaccaaagct cggattgagc
    14341 cggttcggtt gcgagccagc tccgttcgag gcatgtcact gctccgcgca agccggcacg
    14401 cacaaggctg cgcaccacaa tcctcagcag ccgattagcg agtgaacccc tcaccgaatg
    14461 agcccctcga accttcaccg aaatcacgtg agcggaactc gggggctgag gttgacggaa
    14521 gtcagggcct aacgtagctt ccggagccgt gcgtccgagc gcaccccgag tcaccgctcg
    14581 gatgctagaa caccggcgca ggagtccatc caatgggttt gaggcagcga atcatcgaaa
    14641 aaagcaaggg ggtcatgctc aacccctcgg tcatgcgctg ggtgaccgat gaccgtgtga
    14701 tgaaggcgac ggaggggctg ctggacgcac gcacgcgcgt aaaagccgcg gtgcaagtcc
    14761 tgctcaatgg tcacgagctg ccgccagtgg atcctgcgct cgatccgtcc attgcgggga
    14821 tcgagaccgg gcgaggcagt gaagccaaga acggcaacag caacggccac tccgtcgcga
    14881 acggcaacgg taacggcaag gccagtgctt cgattagcat ctccgacgtc agcactcgct
    14941 ccgcgcgcgg ctcggaggac atggacgagt cgctgcgcga gcgcagcacc ttggccagca
    15001 tcggggggcg ggacgtactc gagaaatgcc agaagttcat ggccgcagac aacgcgcgca
    15061 agatgggaat ttaccccttc ttccgcccgc tcgatttgaa cgacggcccc gaggcggtca
    15121 tcaacgggcg ccgagtggtg atgttcggct cgaacaacta cttgggtctc accactcacc
    15181 cacgcgtccg tgaagcttcc aaagccgcca tcgatcgttt cggcaccagt atgacgggct
    15241 cgcgcctcgt gaacggctcg atgaagctgc acgaggagtt cgaggagaag ctcgccgcct
    15301 ggttcggcaa ggagagcgcg ctcgtcttca ccacgggcta tcaagtcaac gtcgcgacct
    15361 gctcggcctt gctgagcaac aagcagagcg ttgccgtcat cgaccgcaac gtgcacgctt
    15421 cgctttacga cggcgtccgg ctcggccaat ccgcaggcgc gcggctcgta cgttaccggc
    15481 acaacgacgc cgagtcactc gacaagacgc tcgagaagct cgaggctggc gaaggcgcct
    15541 tggtgatcac ggacggcgta ttcagcgccg aaggagagat cgcgcatatc gatcaactcg
    15601 tgccgatcgt gaagaaacac ggcgcccgtc tgttcgtaga cgatgcgcat gccctcggtg
    15661 taatcggtcc ggaaggtcgc ggctcatcgc atcacttcgg ggtgtccgcc gaggtcgact
    15721 tgattggggg cacgttctcc aagtcgctgg catcgatcgg cggctacctc gtgggcgaac
    15781 gcaaggtact cgactacatc cggcatttcg caccgagctt catgttcgcg gccgccgcag
    15841 ctccgccctg tgtcgcggcg gccatggccg ccttcgacgt gatgcgcgaa gagtcctggc
    15901 gcatcgacaa gctgcacgaa aacttcaact acatgcgcac cgagctgcaa aagatgggct
    15961 tcgagctcgg gcatactcaa accgcggtaa tccccatctt cgtgcggcaa gatctgcgta
    16021 cgatcatgat gtggcgtgac ttgttggagg agtacggggt ttacaccaat ccgttcatct
    16081 cccccggcgt gcctcccaag agcgccatgc tccgcaccag ctacatggca actcacgagc
    16141 gttcgcacct ggatcgcggg ttggaagcct tcgagaaggt cggcaagaag tacggcgtga
    16201 tcaagtgagg atgaggcggg cacccccgcc tcatccgacg ccgtaagaag agcgtaggcc
    16261 tcgtcgcttc ccgaactcga tattcggact cgacgaggcc tcttcttttt tgccctcccc
    16321 gaacccgaac gcttcgtgcg tatcgcgctg agatcctggc gcgttcgaag gcgagacaag
    16381 acggttttca ggggctggca cggggtcgga tgacagctca ccccgagccc gggaaatcgc
    16441 tgtctttcac gcttcgattg ttccgaatca ccagtgatcg cgcgcggcac gcttcatgaa
    16501 ctcgttccac cccgcgcatg aagaaaccct cggacattca agagctcgcg gacgcgctgg
    16561 gtggtttggc ggtattcggt gtcattccag gcagcccagt agccttgtgc ggagtgcgat
    16621 acggagacgt ggttctaacg accaacggct acaccacacg taccttggac gagttctttc
    16681 gagcccgggc tacgtgtgag agccacctcg agctcgaggt ttttcgagaa ggccggctgc
    16741 tgcgcctgaa cgctcccctc tcttccagcc gcgaactcag cctcgatcag gtctcgagtt
    16801 cggtgatcga cggtgaaatg ctgccaaccg catcgagtcg acggcaggcc tcggtcgtcg
    16861 aatcccgctt tcgctcctga tacacacaca tcgtcttcgc ggtccggacg cggcgaattc
    16921 tcgcttctgg gcgcgccctg agatatgttg ccctctgccc tttggcgatg tcgtcgcagc
    16981 acggccgaag gaggagggaa tggcgcgaca tggaggaggc tcaggtagtt ctgatcgtgg
    17041 acgacgacgt cgtcctcgcg aactccctgt ctcgcgccct gagcggccgt gggctgacgg
    17101 tgttggtcgc ccacgaccgg cacagtgcgc tcggcgtact cggccgcgag cgcgtgcacg
    17161 ccgtgatctt ggacctgcaa acgccgggag cggacggact cgagctgctc gagcgcatgc
    17221 gcgatcgacg cgccgatcgc ccgcgcccgg taatcttgtt gctctcgggt catttggacg
    17281 tgcgcacgac ggtgcgcgcc gtgcgcgccg gcgccttcga cgtgctcgag aagccgatct
    17341 ccgatgcggt gatttacgag cgcctgcggg cggggctgca gcaggggtcg aactcgacct
    17401 ctccgccgtc gaccccaatc tcgcctccgg aaagcaccgg ctcgcccaaa gaccgagatg
    17461 ccgccgatcg gttgctcggc aagaacgcgg cggtacgcgc cgtgcgcgag cagattcgga
    17521 atgtcgcgcg cttccccgat ctatcggtgg tgatcatcgg tgaaacggga acgggcaagg
    17581 agctcgtcgc ggaagcgatt cacatactta ccggctcacg ggggccattc gtgccggtca
    17641 actgcgccgc tattccggaa gctctgttcg aaagccagct gttcggacac gaagcgtttt
    17701 cgttcacggg cgcgcgaggc acccacactg ggctcctagg tgaagcgcat ggcggaacca
    17761 tcttcttcga cgagctcggg gagatgccgc ctcaacttca acccaaactg ttacgagtgc
    17821 tcgagacgcg cagctatcga cggatcggag gcactcgtga cctgccactc caggcgcggg
    17881 taatttcggc caccaatcgg cgcctcacgg ggcgtgaggg ggtggtccgt ccggatctct
    17941 acttccgcct agcgggcttc acgatcttga tgccagcgtt gcgcgagcga acctccgata
    18001 tcgactcgct cgcgacgcac ttcatcgatg agttcgcgcg acgttatccg gaagccccca
    18061 accgcctgac cgagcgggcg ctggaagcgc tgcatgccta cgactggcct gggaatttgc
    18121 gtgaactcaa agcggtggtg cagcaggcgg ccgtcatcac gagcgccagg tcggtcggtg
    18181 ttgccgagat cgcgcgcgtt ctggacgagc gcaacgcgag gcccgcgagc gcccccagcg
    18241 catcacttcg gctcgcgccg acgagcgcgc cgtccgcgga tccggagccc ggaagctccc
    18301 ctcgcgccag tagcccggag ggcccgctgg cgtcgcctcc acacgaaaac cacgccgcgg
    18361 agcggcgccc gcgctcgggg gaacaccgaa tcgagcgtac ggccattcag gacgcattcc
    18421 tagcttcggg gcgcaacctg agcgccacgg cgcgcagctt gggtttgccg cgcactacgc
    18481 ttcgggatcg actccgcaag tatggactct tgtgagctac caacctcgcg agggctagtg
    18541 ctctcgctcg tgatcacacg ttacacattg gccgagcctg ctgttcaagc gcgtcaaggc
    18601 gcgcgctagt gcttcagccg ttccgaccca tggccggctg ctcgcgctcg tctagtttac
    18661 gaagtgcatc gaacaacacg gtctggaacg gccgccgaat actccgctcc ttggcctcgg
    18721 agatcggcga gaccaagggc gtggggcgcg tgtccaagat cgcctggagc gcgtcctctc
    18781 cgtggagccc cccgaacgcc gcgtgcacga tttctccgtc gcacagcacg actcttccga
    18841 ccgtttcgcc gacgaactcg agctcgatac tctgtcgcga gcggtgagcg gcctgcattt
    18901 gatcaatcag cgaacggcgc tccccggggg ccacacccgt ggcggtgtgc gccagcaccg
    18961 ccaagacgtg ctgcggcccg aacggtttgc gcagcacatg ccaaataccg accgcgacgg
    19021 ctcgctttcg cactccgtcc ccggtgtcgc tgcagcacag cacccagtac ggtcgacggt
    19081 tcttgctatc gcccccgccg atgattcgtt ctacatcggc gatcggcaaa cccaagagca
    19141 cgacgtcgaa ccactcggcc ttgagcaagc gccgcgcttc ttcctccgaa cgcgcccagg
    19201 ccacgaacag cccgcgccgc ccgagatccc tcaggagcgc agcgcccaac gcctcgtcat
    19261 cgtcggcaat aagtaccgaa gtcactctac tccctccagc gtcttcgtag ccatgacctt
    19321 gggcaaagtg atctcgaaac aagccccccg ttcgcgtggc acgagttcga gctgaccctc
    19381 gagttcgcgt gcggcgctgc gtgcggtgga caagccgagc cccgatccgg agcgcttggt
    19441 cgagaagaaa ggcaaaaaga tccgttcccg aatctcctcc ggaataccgg gcccgtcatc
    19501 cgccacccga atgcgcacgt gaccttcccc cgccgggggg ggtttggcgc tgacggtcac
    19561 gtgcaacccc gtactcgcgg cctccgccgc attgagcagc aggttgacca agatctgttc
    19621 gatcaagacg gggctgcctc gaacactctc ggcgccctcc gcaatcgaga actcgaggct
    19681 gtggccacgc tcgcgataca cccaacgcac gaagttcctg gctcggccaa acacctcctc
    19741 gagacgcacg tccagacaga cgcgcggccc tccgcgcacg aagtgtagca agccatcgac
    19801 cgcgaactgc aggcgcgcgc tcgccgctct tagatccaac aacgtctcgc gcagtaactc
    19861 cggcggcacg ctctcttcgg cgccccggtc ctccgcgatc agcgcgtcgc tgttgtacac
    19921 gatactggca acggcgctcc gcaggtcatg agcgacgctc gccgtcaatt gcccggtgag
    19981 ctccagcctt tgacgatgca atcgccgctc caccgaggcg tgtgaggcat ccgttctgaa
    20041 cagcagcaac acacgatcgg cgccggcctc gaacgccaca caagatatac ccaacccagt
    20101 ctcgctcgtg ggccagagcc agggggttcc aaagggctgc tcgcgaatgg cgcgaattgc
    20161 cgccatcggt agcacggcct cgctcgagtt tgcggcgcgc tcgagctcgg ggagcagagc
    20221 caaagcctcg ggcgtggcca tgcaaacacg accgagatcg tcgcacaaca acacgggggc
    20281 atcgtgcaac tcgagccatg actcgaggtc gattcgagcg ttcgagctca ctttggagtt
    20341 cacttcgcgg cacccgatgc ggagcacatt cggcccaata agcaatgctc aggccacgat
    20401 ttggatgtcc aagcaaagcc agcagattgc caagtgcgca acgccggata tcacgtgctc
    20461 tccaccagct cgaatcgggt ccgccgacag tccgacatgg cggctcacgc cgaagccgtc
    20521 atgttgcaag cctgtggata gataaacgat acgcaaagtc ccatacgcga tttcacgcac
    20581 ttgccgggcg
//
LOCUS       DASTKU010001075        12538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_658424, whole genome shotgun sequence.
ACCESSION   DASTKU010001075 DASTKU010000000
VERSION     DASTKU010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12538
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658424"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..623)
                     /locus_tag="VFQ61_38235"
     CDS             complement(51..623)
                     /locus_tag="VFQ61_38235"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET9960412.1"
                     /translation="MQLTDRYRRALEFAFELHREQERSGSGVPYFAHLIGVSSLVLEA
                     GADEDVAIAALLHDAAEDQGGTSTLERIEQDFGAKVADIVRACTDAVETPRPPWRERK
                     IRYVEHLKLASPEAQLVAASDKLYNLRTIVADYRSIGEALWSRFSGGRAGVIWYYGAL
                     VEAFTISHGVVAELRRTLAQLETLLTPGSE"
     gene            1381..1453
                     /locus_tag="VFQ61_38240"
     tRNA            1381..1453
                     /locus_tag="VFQ61_38240"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1414..1416,aa:Lys,seq:ttt)
     gene            1504..1574
                     /locus_tag="VFQ61_38245"
     tRNA            1504..1574
                     /locus_tag="VFQ61_38245"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1536..1538,aa:Lys,seq:ctt)
     gene            1606..1679
                     /locus_tag="VFQ61_38250"
     tRNA            1606..1679
                     /locus_tag="VFQ61_38250"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Gln,seq:ctg)
     gene            1767..1853
                     /locus_tag="VFQ61_38255"
     tRNA            1767..1853
                     /locus_tag="VFQ61_38255"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1801..1803,aa:Asn,seq:gtt)
     gene            1859..1931
                     /locus_tag="VFQ61_38260"
     tRNA            1859..1931
                     /locus_tag="VFQ61_38260"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1892..1894,aa:Thr,seq:cgt)
     gene            1942..2016
                     /locus_tag="VFQ61_38265"
     tRNA            1942..2016
                     /locus_tag="VFQ61_38265"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1974..1976,aa:Glu,seq:ttc)
     gene            2149..2221
                     /locus_tag="VFQ61_38270"
     tRNA            2149..2221
                     /locus_tag="VFQ61_38270"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2182..2184,aa:Pro,seq:tgg)
     gene            2224..2299
                     /locus_tag="VFQ61_38275"
     tRNA            2224..2299
                     /locus_tag="VFQ61_38275"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2257..2259,aa:His,seq:gtg)
     gene            2324..2398
                     /locus_tag="VFQ61_38280"
     tRNA            2324..2398
                     /locus_tag="VFQ61_38280"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2357..2359,aa:Asp,seq:gtc)
     gene            2400..2469
                     /locus_tag="VFQ61_38285"
     tRNA            2400..2469
                     /locus_tag="VFQ61_38285"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2431..2433,aa:Trp,seq:cca)
     gene            2471..2540
                     /locus_tag="VFQ61_38290"
     tRNA            2471..2540
                     /locus_tag="VFQ61_38290"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2523..2525,aa:Ser,seq:cga)
     gene            2644..2718
                     /locus_tag="VFQ61_38295"
     tRNA            2644..2718
                     /locus_tag="VFQ61_38295"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2677..2679,aa:Gly,seq:tcc)
     gene            2720..2790
                     /locus_tag="VFQ61_38300"
     tRNA            2720..2790
                     /locus_tag="VFQ61_38300"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2752..2754,aa:Gly,seq:gcc)
     gene            2803..2871
                     /locus_tag="VFQ61_38305"
     tRNA            2803..2871
                     /locus_tag="VFQ61_38305"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2833..2835,aa:Cys,seq:gca)
     gene            2978..3049
                     /locus_tag="VFQ61_38310"
     tRNA            2978..3049
                     /locus_tag="VFQ61_38310"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3011..3013,aa:Glu,seq:ctc)
     gene            3237..3313
                     /locus_tag="VFQ61_38315"
     tRNA            3237..3313
                     /locus_tag="VFQ61_38315"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3270..3272,aa:Met,seq:cat)
     gene            3471..3545
                     /locus_tag="VFQ61_38320"
     tRNA            3471..3545
                     /locus_tag="VFQ61_38320"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3503..3505,aa:Pro,seq:cgg)
     gene            3622..3695
                     /locus_tag="VFQ61_38325"
     tRNA            3622..3695
                     /locus_tag="VFQ61_38325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3656..3658,aa:Leu,seq:taa)
     gene            3783..3855
                     /locus_tag="VFQ61_38330"
     tRNA            3783..3855
                     /locus_tag="VFQ61_38330"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3816..3818,aa:Val,seq:gac)
     gene            3938..4026
                     /locus_tag="VFQ61_38335"
     tRNA            3938..4026
                     /locus_tag="VFQ61_38335"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3972..3974,aa:Leu,seq:cag)
     gene            4028..4109
                     /locus_tag="VFQ61_38340"
     tRNA            4028..4109
                     /locus_tag="VFQ61_38340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4061..4063,aa:Leu,seq:gag)
     gene            4117..4204
                     /locus_tag="VFQ61_38345"
     tRNA            4117..4204
                     /locus_tag="VFQ61_38345"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4151..4153,aa:Leu,seq:caa)
     gene            4320..4829
                     /locus_tag="VFQ61_38350"
     CDS             4320..4829
                     /locus_tag="VFQ61_38350"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET9960413.1"
                     /translation="MNHRTLLLSPWYVPIQILQWQDAVKMRYEGTAEVIAEYEQEICS
                     PSVKWHIPAVMRLRRMPRAKQRGIKFSRVNVYQRDNFCCQYCGKRFPVGMLSYDHVVP
                     RAAGGRTCWENIVTACRACNTRKGHQSCDAAGMWPMKDPWKPKTLPFAGPLIDRGQAP
                     DEWLAFLPA"
     gene            5396..5668
                     /locus_tag="VFQ61_38355"
     CDS             5396..5668
                     /locus_tag="VFQ61_38355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960414.1"
                     /translation="MNPNSLTRGAAIVAAAAALLTQACSSSDDDAAAKTDALKCLGAN
                     ECKGMSECAGGLGMSGCKGLNECKGMGWIYTDTEADCTAKGGTVQS"
     gene            5678..6499
                     /locus_tag="VFQ61_38360"
     CDS             5678..6499
                     /locus_tag="VFQ61_38360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HET9960415.1"
                     /translation="MNAGFGLRVPHYEELIVRGTRASFVEAVSENFLGRGGRPRHLLE
                     RIRRDARLALHGVSLSIGSVDPLNCDYVAELASLARECEATLVSDHLCFASIGGHYAH
                     DLWPLPYTDEALEWVVDRARAVAARLPCPFLLENVSSYVEYEASTLSEWEFLGEVCER
                     ADIGILLDLNNVVVSAKNHGFSAEKYVDALPRGRVLQIHLAGHTDWGTHAIDDHASAV
                     SDEVWSLYRRALQRFGPVPTIVEWDDAIPPLATLEAEASKAATLQKQQAHVSGGS"
     gene            6480..7295
                     /locus_tag="VFQ61_38365"
     CDS             6480..7295
                     /locus_tag="VFQ61_38365"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HET9960416.1"
                     /translation="MSLADLERAFWNCVRSRTPPPAGSGFVERPPLSAARRVGVYHHA
                     YFARQQGVLEELFPRVRAALGPARFRALAQEYLMEIPSRTPIIERIGAQFPEFLRGLG
                     AEEPVLGELAHLEWAHLETLLAPDGPTMHAEELARVEVERARWIAAPCVHLLRVMTGA
                     LDLWNPPGDSAIAGGRGRPPNPDEHHTEYPTPEGDQRRRFVVVTRVRDAVIHAVLSDE
                     AGRALERLLSGATIIEAFCEFDDGPMGVAQAGATLAEFTRLGLWSRWEVVDGN"
     gene            7285..7815
                     /locus_tag="VFQ61_38370"
     CDS             7285..7815
                     /locus_tag="VFQ61_38370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HET9960417.1"
                     /translation="MAIESPFARRLRISAFCLFALGCGSEAHSGEFETGPLMKPGDDC
                     LRCHSHGSAYPQAQPWSLAGTVFPRPNAAADEGVPGVRLTVTDDEGNLVTSLTSNSVG
                     NFYTPVTLPQPFRVALEYEGRSVEMPCPPPAGNCGACHSLPPIGGPLGRVSIAQGLPE
                     LEGSFNCVQWRREPGQ"
     gene            7856..8467
                     /locus_tag="VFQ61_38375"
     CDS             7856..8467
                     /locus_tag="VFQ61_38375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9960418.1"
                     /translation="MRSDEELMVAHTAGDRDAFAELFRRYAPPLERVLQAQFLARSEV
                     EDLIQQTFLQVHRARHDFDPLQSFRPWLFTIAFNLKRELYRKRKRRPEAVLDENAPEL
                     ATASVHHARSENLNALDRGLSKLPPDQHEVITLHWFGGLSFPEVARAVGATTAAVKVR
                     AHRGYVSLRRVLEDLPGGDANARSAAGSVPTNSQSAGGRRGVR"
     gene            8484..9260
                     /locus_tag="VFQ61_38380"
     CDS             8484..9260
                     /locus_tag="VFQ61_38380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960419.1"
                     /translation="MSGHTPSGAALEEHLRQCPACRALCEDGAGLGQLLARAASLDNS
                     GSDALLSRLTSTVDAEVGLRARLRAASTRKRTMIVAGVGLVAVLIPSLSKARPSWPPV
                     GEFVASLFALFALAALCVRFALRPLSEARKSMLSTLLAAISLAVPFLLAIVAPFASRA
                     PEGHAPHAWACFSFGAAFSFPVLLVLRLLERSERPPILALVLSAATTGLVANAVLALH
                     CPSDDSLHLLLGHASIGAGWLLAAACQRVVTSRARRGPPE"
     gene            complement(9321..11267)
                     /locus_tag="VFQ61_38385"
     CDS             complement(9321..11267)
                     /locus_tag="VFQ61_38385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET9960420.1"
                     /translation="MSLGTFLFGEPLATEESEGQTVGPLAGVPILGLDALASAAYGPE
                     ALLTVLLPLGTHALAHLQGLTLLIVALLVILFFSYRQTIAAYPTGGGAYTVAQENLGT
                     GASLFAAAALALDYILNCAVAISAGVGALVSAVPVLLPYTLLLCLAILGLLTLMNLRG
                     VRTTGLAFMLPTYSFIGCLLAVIGWGIYRSYVLHAEPIVPPSRALGHLADASPWLLLH
                     AFASGCTAMTGVEAVSNGVPVFKRPSVVHAQRTLAIIIAVLLLLLVGEAVLCRSYAIT
                     ATLPGEPGYQSVLSQLTAAVFGKGAAYYLTIASVTTVLCLSANTSFADFPRLCSLLAR
                     DKFLPEPLVHRGRRLTFSHGIWILSALSALLLVVFGGVTEGLIPLFAVGALFAFTMSQ
                     AGMVMHWRKRGGLRPSLVLNGIGALATSITLCIVLAAKFFEGAWLTVLIVALMIVLFR
                     NVQRHYEFLSRVTALDVQPDFSPPKRPLAVVPLRRWDAIALKALRFALGFSPEVKVVQ
                     VLTNDRSVDDLTARWREWVKIPTRAVGLEAPELVVLHSQYRELFSPLINYINELSAAN
                     PDRQIAVIIPELVERRWYHYALHNHSASFLKALLLWRGSPQIVIVNVPWYLSQWRPER
                     AQLAGTGRMLRRLWQRNHGNGVKV"
     gene            complement(11403..>12538)
                     /locus_tag="VFQ61_38390"
     CDS             complement(11403..>12538)
                     /locus_tag="VFQ61_38390"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET9960421.1"
                     /translation="SLLLERLSEVAVLISLFTAGLKLNAPFEDSRWALPFRLAFVSMA
                     ASVGLTTLVGVMVMGLPLGVAVLCGAVLAPTDPVLASDVQVEHAGDRDRLRFALTGEA
                     GLNDGSTLPFILLGLGLLGLHPLGETGWRWLLLDVLWSTAAGAAIGFLIGTSIGRVVV
                     HLRTRHKEALGLDEFLTLGLIALAYGVAVICSSNGFLSVFAAGLALRRIQARHKQSEL
                     APQGVSRAELAASPEQAPTYMTEAVLDFNEQLERLGELTLVLLVAGTLRLDQTPEAAL
                     WFVPLLLGCIRPLSVWLGTLASRSTGQQRALMGWFGIRGIGSVYYLMFIINHGLPEPY
                     RSLLVGLTLTTVAVSIVVHGISVTPLMNQYTRMNRLVRRRAKA"
BASE COUNT         2425 a         3876 c         3894 g         2343 t
ORIGIN      
        1 ctctggcgtg cactgtgact ctggcgtgat ccgcactctg cttgacctgc tcactcggac
       61 cccggcgtca atagcgtctc gagttgagcc aaggtgcgac gcaactcagc cactacacca
      121 tggctgatcg taaaggcttc gacgagggct ccgtaatacc atatgacgcc tgcgcgcccc
      181 ccactgaaac gactccacag ggcttcaccg atgctccgat agtctgccac gatcgtgcgc
      241 aggttataca gcttgtcact ggccgccacg agctgcgcct ctggggaggc gagcttcagg
      301 tgctcgacgt agcgaatctt tcgttctcgc caaggaggcc tgggcgtctc gacggcatcg
      361 gtgcaggcgc gaacgatgtc agccaccttg gccccgaagt cctgctcgat gcgctcgagt
      421 gtgctcgttc caccttggtc ttcggcggcg tcgtgcagga gggcggcgat cgctacgtct
      481 tcgtccgcgc ccgcttcgag caccaagctc gagactccga tcaaatgggc aaagtacggc
      541 acacccgatc cgcttcgctc ctgttcgcga tgtagttcga acgcgaactc gagtgctcgc
      601 cgataacgat ccgtaagctg catttgtcac ctctccttct cgaatgcgtg cctcaatggc
      661 gaatcaatca gttccgcccc gcagcctagc caacgcggga agccatggcc acgaaaagag
      721 ccggggagcg cgcggcgctg ggcgcgactg ctgacgtatt cagtccccgt gcagtctgct
      781 tgtcattcta gatccgctgc cctcgaagcc cgcagacgca agctagcctg catccccgta
      841 ccccgtacga agactctccc gtgagttatc gaaagcatca cgaacaccgc tagtcctcgt
      901 tgccgtccta ttgaagagtc ctattcgaat tacctacacg aacaaaaatc caaatccttg
      961 cttgaccact ttgaggagat cgttcataaa cgaaccctct cacatgttgc atctcgacct
     1021 atccaccatc cccggacgcc gctttggcgc atgggaatcg tgtatggtcg tgtatcaggc
     1081 cggaagcgat cgttctgcca aggagaatcc ggcgtagcgg gcggtatttc cacgtattta
     1141 cgtgaaagcc gcccagggaa acctcggcgg cttttttcgt ttctgagctt tgttctgggt
     1201 gcctggctcg tccggcactt ctttgctctg gtcccggggt tcgtcctcgg aaggcgactg
     1261 gcgctgtaaa cgccgggcca ttggctagac cgttcgactc ggtacgggac cactatgcat
     1321 atttcatcga atgacatcgt cacaaaacac agaatctgac taggtcagtt tcgtatgttg
     1381 ggtggcgtaa ctcaataggt agagaactcg acttttactc gaggcgatgc acgttcgatc
     1441 cgtgtcgcca ccacttgcgg ctggagctcg acctttgtcc gcatgggtca agctccgggt
     1501 tcagggcctg tagctcagag gcagagcagc tgattcttaa tcagtcggac tgagttcgac
     1561 tctcagcagg cccactacga gtgtgctcgt tcgcgtgcac tccattccgg gatcgtctag
     1621 cggcaggacg cgagtttctg aagctcgcta cgttggttcg aatccagctc ccggaaccac
     1681 gagtccaatg actcgcgatc tatgacaatc tgaacacggc gtgacggcgc gctctgcgtg
     1741 tggcggtctc ctgtgatgtt cgatattccc gggtacccga gaggaagcag ggagcggatt
     1801 gttaatccgc cgggcctcgt gccccgtcgt tggttcgaat ccaaccctgg gagctattgc
     1861 cgacgtcgcg cgcgtgggtg cgcacctcat tcgtaaagag gactaggctg gttcgactcc
     1921 agtcgtcggc tctgctggtt tgccgcgttt tctgctggtg cggtggctgg gttttcaccc
     1981 cagtttcggc gggatcgtaa cccgcacgcg gctccatact cagcgccatc ggaggttggc
     2041 gtcagccatc cgactactca gggcgtcgag taacgggttt caagctgatc tggtgacagc
     2101 gccggtctga aaaaccggag agcgcggatc gtaaccgcgg aaacccgccg gggtatagcc
     2161 tagtggccca ggcacctgat ttgggatcag gatcaggcag gttcgattcc tgctaccccg
     2221 actgcgacgt gagcgagcag gtgaacgcgg ccggctgtga accggcttcc gacgggatcg
     2281 ttacccgtac gtcgccccac gttcctgtac ttaggaacgc catgcagcgt gatgcgactg
     2341 gtgtcgcaga ccgattgtcg atcggttgag acgagttcga tcctcgtacg ctgcgccata
     2401 cgaccgagct caggctgagc aatgtagcct ccaaaactgc agacgagggt tcgagtcctt
     2461 ccggtcgtgc ggggtcgtcc gttgggacgg ggcccttcct tgcaagaagg cgcatgcgag
     2521 ttcgattctc gacgactcca ccgaatcttt ggccgacccg gccaagcaaa cgctcatgta
     2581 tctggtgatg ggtggccgtt ggtaacggcg acccccgagt tcgatcctcg gcgtgagctc
     2641 caagcgcgtt tggtgtcaac ggcagcacag ctggcttcca cccagcaggt gacggctcga
     2701 gtccgtcaac gcgcaccacg cagccaagat gttacggaag catgcgacgt tgccaacgtc
     2761 gacgagcgag ttcgattctc gctggctgca ctattgtttt ttggcgcgct tctcaggcga
     2821 ggtgcaccga ttgcaacccg gtgaaggacg gttcgagtcc gtcgcgcgtc tccgctctgt
     2881 tcatgtccaa aggtaattcc tcaatcgccc acactgagga cagcgcgcgc tcttctaaag
     2941 cgctgcggtt aggttcgatt cctgatgtgg gcaccctgtc gcattcgtct agcggccagg
     3001 acggcggact ctcgatccgt ctacgggggt tcgattcccc catgcgacgc tgtgtcactg
     3061 gttcatggcc gcttctcggc tcatgccctc gcccctgccg gcggatccgg cgccgacgct
     3121 tcgaacgttg gctagagtgt tcgactcatt ccaggggcgc cctgctctcg ccagcggatt
     3181 ctggcggcgg ccgtgcgaag gctgcatgcg gtggttcgac tccactcgag agctctagcg
     3241 agaatggagc aagagcggct cgcgtgcctc ataagcacga aatcgcccga ggcaggttcg
     3301 ggtctcgctt ccaatcgcca tgacgttcgg aacgctcgcg ctttcatacg gcgtcgagtc
     3361 gcggttcgac tccgcgcgtg gcgaccacat tccggggtcg tctatcggca ggataacttc
     3421 gctttgaacg aaggagagca gcgttcgagt cgctgacccg gagctatcat cagggagatt
     3481 gcgcgaggtg tgcacccggc gtcggaagcc ggtgtcggca ggttcgactc ctgcctccct
     3541 gaccatactg ggcgagagca gcaagtgaag ccgtcggtcc tacaaaccga agttgcgcgg
     3601 agcgttaccg tggcccagta cgcgcatcta caccaatcgg tagagtgacc gcccttaaaa
     3661 cgcggctagt ctcggttcga atccgaggat gcgcacccat gctgcagagc gcaaggaact
     3721 ggcgttctgg gagttctgat aaagctcccg cgctgggttc gattcccagg cgctctacca
     3781 aagcgagacg agccagctgg tgacggcacg agcccgacaa gctcgaccag gtgagttcga
     3841 ttctcacgtc tcgcaccgga ggtcagcctc caggcgcgct ccacgccgtg aaaagagaac
     3901 ctgcatcctg ttggagatgg gttctaggga gcaccaagct cgagtggcgg aacaggcaga
     3961 cgcgccgcgc tcagaacgcg gagacccttc gtggtcgtga aggttcgact cctttctcga
     4021 gcaccacgcc tcagtggtgg aatggcatac acggcaggct gagaacctgc gccctgcggg
     4081 gcatccgagt tcgagtctcg gctgaggcac ccacatccta gcctggtgaa attggcaaac
     4141 acgctcggct caaacccgag tacctctaac cgaggacctg cgggttcgaa tcccgcggct
     4201 aggacccttg agctccggtg gattcttcgg cggcgacgct gaagccgtcg ttcacgcgac
     4261 gtttggacat ccaccggagc ccgagaacca atgcgcgatc caaagcgctg gaggcacccg
     4321 tgaaccacag aacattgctg cttagcccct ggtacgtccc catacagatc ctgcaatggc
     4381 aggacgccgt gaagatgcgc tacgaaggca cggccgaagt catcgccgag tacgaacagg
     4441 aaatctgctc accgtccgtc aagtggcaca ttcccgccgt gatgcggctg cgacgcatgc
     4501 cccgggcgaa gcagcgtgga atcaagttct cccgagtgaa tgtgtatcag cgtgacaact
     4561 tctgctgcca atattgcggc aagcgcttcc cggtcgggat gctctcgtac gatcacgtcg
     4621 taccgcgcgc tgcgggcggt cgcacttgtt gggaaaacat cgtcacggca tgccgcgcgt
     4681 gcaatacgcg caaggggcac cagtcctgcg atgccgcagg gatgtggccc atgaaagatc
     4741 cctggaagcc gaagacgctt ccgttcgcgg gccccttgat cgatcgcggt caagcgcccg
     4801 atgagtggct ggcattctta ccggcataac gcccgcctgg ggcgtcaccc aggcacttgg
     4861 agagcaaacc gaacagggtc tcggcgacgt ttggaaggcg tcaggtgctc cggcatgtgg
     4921 atcgtgtcca ctgctctccg ccttggatgg tgcggctcag cggtgagcaa ccggatttga
     4981 accccggtgg ggcgcgcaag cgcaacagtt cgactctgtc gccatccgcc gcgctccgcc
     5041 agacgcgaag cgtgaacaat ggaagctgaa ccgtgcgagt gcacgggcct gattcgaacc
     5101 agtgcgcagc cactccggct gtggggcgcg agacctccgg cttccgcaaa gtatggaacc
     5161 tgatctcggt gtggaccgag gcctgcctgc taagcagtgc gcactccagt gtggtgttcg
     5221 actcacccgg gttccgcgaa tagtccccct atctcctccg caggctaccg gctactaaac
     5281 gggcgtccgg gcaaagctct accggaaaga caaaaatcgc cctcgcgccg taaccgctcg
     5341 cgaacctcgg catacgcgaa accgagaccc tgatgtctcg agaggaacgc aaagcatgaa
     5401 ccccaacagt cttacccgtg gtgcggccat cgtcgcggcc gccgccgcat tgctgacgca
     5461 ggcctgtagt tcgtcggacg acgatgcggc cgccaagacg gacgcactga agtgccttgg
     5521 cgcgaacgag tgcaagggca tgagcgagtg cgccggaggg ctgggtatga gcggctgcaa
     5581 agggctgaac gagtgcaaag gtatgggctg gatctatacg gatacggagg cggactgcac
     5641 ggccaaggga ggtacggttc agagctgaga ggccacgatg aacgccgggt ttggactgcg
     5701 cgtgccgcac tacgaagagc tgattgttcg cggcactcgc gcttcgttcg tggaagccgt
     5761 tagcgagaac tttttaggtc gtggaggccg cccgcggcac ctgttggagc gaatccgacg
     5821 tgatgcgcgt ctcgccctgc acggggtctc gctctcgatc ggttcggtgg acccgctcaa
     5881 ctgcgactac gtggccgaac tcgccagttt ggctcgagaa tgcgaggcaa ctctggtctc
     5941 ggaccatctg tgcttcgcca gtatcggcgg gcactacgcg cacgacctct ggccgcttcc
     6001 ctataccgat gaagcattgg agtgggtcgt ggatcgcgcg cgggcggtgg cggcgcggct
     6061 gccttgcccg ttcttgctcg agaacgtatc gagctacgtc gagtacgagg cgagcacgct
     6121 cagcgaatgg gagtttctcg gcgaggtgtg cgagcgggcg gacatcggga tcctcctgga
     6181 cctgaacaac gttgtcgttt cagccaagaa tcatggattc tctgccgaga agtacgtgga
     6241 tgctctgcca cgagggcgtg tgctccaaat ccacctcgcc gggcacacgg attggggaac
     6301 ccacgcgatc gatgatcacg cctcggcggt ttcggacgaa gtctggtcgc tctatcgccg
     6361 ggctctccag cggtttggcc ccgtgcccac catcgtcgag tgggatgatg caataccgcc
     6421 gttagcaact ctggaggccg aagcatccaa ggcagctacg ctgcagaaac agcaagcgca
     6481 tgtctctggc ggatcttgaa cgcgcctttt ggaactgcgt gcgcagtcgc acaccgcccc
     6541 cggcgggcag cggtttcgtt gaaagaccgc cgctcagcgc cgcacggcgc gttggtgttt
     6601 accatcacgc ctacttcgcg cgccagcagg gcgtactgga ggagctcttc ccgcgagtgc
     6661 gagccgcgct cggcccggct cgatttcgcg ctctggccca agagtatttg atggagattc
     6721 catcgcgcac gcccatcatc gagcgcatcg gcgcgcagtt tcccgagttc ttgcgaggtc
     6781 tgggcgcaga agagccggta cttggcgagc tggcgcatct agagtgggcg cacttggaaa
     6841 cgctgctcgc cccggacggt ccaaccatgc acgcagaaga gctcgctcgg gtggaagtcg
     6901 aacgagcacg ctggattgca gcgccttgtg tccacctact ccgggtcatg acgggagcct
     6961 tggacctgtg gaacccgccg ggggactctg ccatcgcggg aggccgtggc cggcccccga
     7021 accccgacga acatcacacg gaatacccta cgcctgaggg ggaccagcgg cggaggttcg
     7081 tggtcgtaac tcgcgtgcgc gacgcggtga ttcacgccgt gctctcggat gaagcgggtc
     7141 gagctttgga gcggctgttg tcgggggcca ccatcatcga ggcattttgc gagttcgatg
     7201 atggtccaat gggcgtggcg caagcgggag cgacactcgc agaattcacg agactcgggc
     7261 tctggtcgag gtgggaggtc gtcgatggca attgaatcgc ccttcgcgag gagactccgc
     7321 atatcagctt tctgcttgtt cgctctcggc tgcggctccg aggcccactc cggcgagttc
     7381 gaaacaggtc ccttgatgaa gccaggggac gattgcttgc gctgtcactc acacggctct
     7441 gcctatccgc aggcgcagcc ctggtccttg gcggggacgg tgtttcccag accgaatgcc
     7501 gcggccgacg agggcgtgcc cggtgtacga ctcaccgtaa cggatgacga gggaaacctc
     7561 gtcacgagcc tcaccagcaa cagcgttggc aatttctaca ctccggtgac gctgccgcag
     7621 ccgttccgcg tggccctcga atacgagggt cgaagcgtgg agatgccgtg ccccccgccc
     7681 gccgggaact gcggcgcctg tcactcgctg ccccccatcg gtggcccgtt gggaagagtc
     7741 tctatcgccc aggggctccc tgagctggag gggtccttca actgtgttca gtggcggcgt
     7801 gaaccgggac agtgagcacc tgacaaacgc gcgcgggcta tgtagtctgc cgccggtgcg
     7861 gagcgacgag gagctcatgg tagcccacac ggctggcgat cgagatgcgt tcgccgaact
     7921 ctttcggcgc tatgctccgc cgctcgaacg cgtcctccaa gctcaatttc ttgctcgctc
     7981 cgaggtcgag gacctgattc agcagacgtt tctacaagtg caccgtgccc gccacgactt
     8041 cgatccctta cagagcttcc gtccttggct cttcacgatc gctttcaatt tgaagcgtga
     8101 actctaccgt aagcgcaagc gccgtccgga agccgttctc gacgagaacg cgcccgaact
     8161 cgccaccgcc agcgtccatc atgcgcgctc agagaatctg aatgctctgg atcgcgggct
     8221 ctccaagctg ccgcctgacc aacacgaggt catcacgctg cactggtttg gcggactgag
     8281 ctttcccgaa gtggcgcgcg ccgttggggc gaccacggcc gccgtcaagg tccgcgccca
     8341 ccgtggctac gtgagcctgc gaagggttct agaagattta ccaggaggcg atgccaacgc
     8401 gcgttcggca gcgggcagcg tgcccacgaa ctctcagagt gcgggaggac gacgtggggt
     8461 gcgctgaaat tcggaccgcg ctcatgagcg gccatacccc ctccggcgcc gctctggagg
     8521 agcacctccg tcaatgtcca gcctgtcgcg cgctgtgcga ggacggcgct ggtttgggtc
     8581 aactactggc acgagctgct tctctggata acagcggttc cgacgccctg ctttctcgac
     8641 tgacgagcac tgtggacgcc gaggttgggc tgcgtgctcg gctgcgcgcg gcgagcaccc
     8701 ggaagcgaac catgatcgtg gcgggggtgg gactggtggc ggtgctgatt ccctcgctct
     8761 cgaaggcgcg cccttcttgg cctccggtcg gcgagttcgt cgcgagtctg tttgcgttgt
     8821 tcgcgctggc ggcgctatgc gtgcgtttcg cactacggcc actgtcggaa gctcggaaat
     8881 ccatgctgag cacgctgctg gccgccatct cacttgcagt tccgtttctg ctcgcgatag
     8941 tcgcgccgtt tgcttctcga gcgccggagg ggcatgcgcc gcatgcttgg gcttgcttct
     9001 ctttcggagc agccttttct ttcccggtct tgttggtgct gaggcttctc gagcgaagtg
     9061 aacgcccccc gatcctggca ctcgttctga gcgcagcgac taccgggctc gtggcaaacg
     9121 cggtgctagc gctacattgt ccgagcgacg actcgcttca cttgttgctc gggcacgcca
     9181 gcattggtgc gggttggctg ctcgcagcag cttgtcaaag agtcgtaacc agtcgtgcgc
     9241 gtagaggccc cccggagtga gaggcatcgc gcagggttgg cccgggcgcg cggcatcgaa
     9301 tgctgggctc taaaatcgcg tcagactttg acgccattgc cgtggttacg ctgccagagc
     9361 cgtcgtagca tccggcccgt tcccgcgagc tgagcgcgct ctggacgcca ttggctcaga
     9421 taccaaggga cattcacgat cacgatttgc ggtgaccccc gccacaagag cagcgccttg
     9481 aggaaggacg ccgagtgatt gtgcaatgca tagtgatacc agcgacgttc gaccagttct
     9541 gggatgatca cggcaatctg gcggtcgggg ttggccgccg acaactcgtt gatgtagttg
     9601 atgaggggcg agaatagctc ccggtattgc gagtgcagca cgacgagttc aggcgcttcc
     9661 agacccacgg cgcgggtcgg gatcttgacc cactcgcgcc aacgggccgt gaggtcgtcg
     9721 acgctgcgat cgttcgtcag cacctgcacc accttcacct cgggcgagaa ccccaacgcg
     9781 aagcgcagag ccttcaaggc gatcgcgtcc cagcgccgca gcggaactac ggccaatggc
     9841 cgcttgggcg ggctgaagtc gggctgcaca tcgagtgccg tgacgcggct caagaactcg
     9901 tagtggcgtt gaacgttccg aaagagaacg atcatgaggg cgacaatcaa gaccgtgagc
     9961 caagctcctt cgaagaactt cgcggccagc acgatgcaca acgtaatact ggtggccagc
    10021 gcaccgatgc cgttgaggac gagcgacggt cgcaggcccc cgcgttttcg ccaatgcatc
    10081 accatgccgg cttgggacat cgtgaatgcg aacagcgccc ccaccgcgaa cagcgggatc
    10141 aacccttccg ttacgccccc gaacacgacc aggagcaatg cggagagcgc gctcaagatc
    10201 cagataccat gtgagaaagt caacctgcgg ccgcgatgca cgagcggctc gggcaagaac
    10261 ttgtcgcgcg ccagcagcga acacaggcgt gggaagtccg cgaagctggt gttcgccgac
    10321 aagcaaagca cggtcgtgac gctggcgatc gtcagataat aggccgcgcc cttgccgaac
    10381 actgccgcgg tcagctgcga gaggacactc tggtaacctg gttcaccggg cagcgtcgcc
    10441 gtgatcgcgt aagagcgaca cagtacggcc tcgcccacca ggagcaacag caatacggcg
    10501 atgatgatgg ccaaggtgcg ctgcgcgtgg acgacactcg ggcgcttgaa cacgggcacg
    10561 ccattgctga ccgcttcgac gcccgtcatc gccgtacagc cgctggcgaa ggcgtgcagc
    10621 agcagccagg gcgaagcgtc cgccaagtgc ccgagcgcgc gtgacggggg cacgatgggc
    10681 tcggcgtgca gcacgtaact gcgatagatg ccccacccaa tcacggcgag caggcaacca
    10741 atgaaggagt aagtgggcag catgaacgcg agccccgtcg tacgcactcc ccgcagattc
    10801 atcagggtca gcaagcccaa gatggcgaga cacagaagta gcgtgtacgg aagcagcacg
    10861 ggcacggcag aaaccaaggc tcccacccca gccgagatgg ccacggcaca gttgaggata
    10921 tagtcgaggg ccaatgcggc tgccgcaaac agcgaggcac ccgttccgag gttttcctga
    10981 gcgacggtat atgcccctcc accggtggga tatgcggcga tggtctgccg gtaggagaag
    11041 aacaagatta cgagcaaggc gacgatcagc agtgttagcc cttgcaagtg agcgagtgcg
    11101 tgcgttccga ggggcaacag caccgtcaat agcgcttcgg gtccgtacgc ggccgatgcc
    11161 agcgcatcca aacccagaat gggtaccccg gcgagtggcc ccactgtctg tccctcgctc
    11221 tcctcggttg cgagcggctc accaaacaga aaggtcccta gagacattcg gctcacgacg
    11281 ttgccgtttc agcaaaaatg atgccgactc agctgggcac gacgctcccc ccacgccaat
    11341 cggtctcgca gttcgaggtc cgcgagcgga tggttacgaa aggcttcgtt gcgtcaaaat
    11401 gctcaggcct tggcgcgacg acgaactaga cgattcatgc gcgtatactg gttcatgagc
    11461 ggggttacgg agatgccgtg cacgacgatc gaaacagcca cggtcgtgag cgtgagacca
    11521 accaacaagc tccgataagg ctccggcagc ccgtggttga tgatgaacat caagtagtag
    11581 accgagccga tgcctcggat cccaaaccag cccatcaggg cgcgctgttg acccgtgctc
    11641 cgcgaagcta acgttcccaa ccagaccgag agcggacgaa tgcaccccaa tagcaaaggc
    11701 acgaaccaca aagcggcctc cggcgtctgg tcgaggcgta aagtacccgc gacgagcagc
    11761 acgagtgtca gctccccgag acgctcgagt tgctcgttga agtcgagtac ggcctccgtc
    11821 atgtaggtgg gtgcctgctc gggagatgcc gcgagttcgg cgcgcgatac gccttgcggc
    11881 gcgagttcgc tttgcttgtg acgagcttgg atgcgccgaa gcgcgagccc agccgcgaag
    11941 acagacagaa agccattcga cgaacagatc acggcgacgc catatgccag cgcgatgagc
    12001 ccgagcgtca gaaactcgtc cagaccgagc gcctccttgt gccgtgtgcg caggtgcacg
    12061 accactcgac cgatcgaagt tccaatcaga aagccgatcg cggcgccagc ggccgtggac
    12121 caaagcacgt cgagcaaaag ccaacgccag ccagtctcac ccaacgggtg taacccgagc
    12181 aggccgagcc cgagcagaat gaagggcagc gtgctgccgt cattgagccc cgcctcccca
    12241 gttagcgcga agcgcaagcg atctctatct cctgcgtgct caacctgaac gtcggaagcg
    12301 agcacggggt cggtaggagc gagcaccgcg ccgcaaagca ctgcgactcc cagcggcaat
    12361 cccatcacca ttacgccgac caaagtcgtg agccccaccg aagccgccat ggatacgaag
    12421 gccagacgaa acgggagcgc ccaccggctg tcctcgaacg gcgcattcaa tttgagcccc
    12481 gccgtgaaca aagagatgag gaccgcgacc tcgctcaaac gctcgagcaa tagcgaat
//
LOCUS       DASTKU010001076        10983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_213080, whole genome shotgun sequence.
ACCESSION   DASTKU010001076 DASTKU010000000
VERSION     DASTKU010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10983
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213080"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VFQ61_38395"
     CDS             <1..1101
                     /locus_tag="VFQ61_38395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HET9960422.1"
                     /translation="IGVVVEAATTDQIMKDVSSITKDVKRVTEALAASVGTDTGRENI
                     KDILENLARVTESLNNTVRENRESFRNIMVNVERITAEGRPELREILENVRASTAEVR
                     GLLDKSDEQTAGAKPGEVRQIIEKVNRASTNLESALKNIDEVSGRLERGEGTLGRLTK
                     DEKLIDEVQGVAETVGDFLGGVSRLQTILVLRTDYQFLNSTVKSYVELRLQPREDKYY
                     LIEVVNDPRGLTRFEQVVVDTDNPNDPPHYRELRTVTTNAFRFSLQFAQRFGPFTGRF
                     GIKESTGGVGLDTSLFQDRFELRQDLYGFGEVVLPRWRVSLGYEFIHRLWLLGGVEDI
                     LSKARRDYFVGAQLKFTDEDLKTILPFAPAGP"
     gene            complement(1117..3321)
                     /locus_tag="VFQ61_38400"
     CDS             complement(1117..3321)
                     /locus_tag="VFQ61_38400"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9960423.1"
                     /translation="MRPEQHPRDQPLDAQRKEGLPSESARNDFAQATQPQPSQPQPSQ
                     PQPSQPQPSEALPAESHGARVDVQKPFGPFVLERRIAVGGSAEVFLARPKVGTVPAAH
                     LVVKRLLPNARESGFDVLEREAELHRCVAHANVVTVFGAGMVGNEPYLAMEYVDGVDL
                     YRLLRRAEAEQRRIPPGLAVYVARRVLQALAAVHAAVDREGKPLYIVHRDVTPSNVYL
                     SVEGHVKLGDFGIARTEDRSEPAAPGGLKGKFGYLAPEQVAGEAFDHRADLFALAALL
                     GEMLLGERVFPGSGQLAVLLAIRDANIEPLRSAARSFPPGLFAVLEKALARSPDDRYA
                     AADDLARALEPFERPNPIDLKVKLSEWVTWARDSSQFARKLEGQIRESVSRMQAVKST
                     GHPHHRATPVLETLAPTETASRLRRSGAHHVETLSFAKLIEMVATGELCGDDEVALMG
                     AELKPIRDIHELARHLLPSTTATTSRVFEPGAPDYSVLLRDTSMLEVLAHLRTRGMSG
                     ALFVQRTLGSGRASRKEMYLEQGRLHHVASSEREELLGEYLVRRGKLSREQLETGLGA
                     LARFGGRLGDTLIGLGLVDAVDVFRAIRDQGRDRVAALCTWQEGLVSFYSGTGATRVD
                     FPLDLDLASPMMAGAILAADGEPRALLPLGSTIVLPGLRAEATKNRKERGTAPTSLHL
                     VSTLVFERLSVERIIERLTSYHPRNDARIITSKEACAALVTAQALSWIQWPK"
     gene            3551..5230
                     /locus_tag="VFQ61_38405"
     CDS             3551..5230
                     /locus_tag="VFQ61_38405"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HET9960424.1"
                     /translation="MIVKRPSLFWRKARSLGSDAAHAGAVRPSLRLISALVFVILASV
                     VSCRCRPSPVKSESAQVPSLRLVLLSSVAGALEPCGCVKDMLGGIDHAAAYLSSSKGP
                     PSLVLGAGPMLFMDPVLHPERRTQDVWKAETMNRSLASMGLKVWAPAVNDFAAGSEEL
                     ARIAALGARPLSANLKSQGLALEGHKLFRVGGVSVGVAAASAPKHLGKSPPGVELGEV
                     ESALQQQARELAQKGAQLRVVLLALPRGEALRVIEKVPDFQIALIGKAVDQGESNDPV
                     TPTTRVGNTLVVESPNHLQGFYVVDLFLRDGKFELENGDARSDERAALEVRIRELEQR
                     IAQAEATGNVQRSDLDARRADLTKLRDQRARLTGSSRPAHGSYYQARLVEVRESLGTD
                     PGQTAQLSEYYKRVNEHNRVAFRDRVPAAPAADESGYVGVQVCGACHTQALAVWQKTR
                     HSRAYETLVAGSKQYNLDCVGCHVTGYEKPGGSTVAHVGNLASVQCEVCHGPGSRHIA
                     SVSDKTLIVAKPDPGLCAANCHHEPHVHPSWNVAAALPKIIQPGHGISLAK"
     gene            complement(5350..7404)
                     /gene="tkt"
                     /locus_tag="VFQ61_38410"
     CDS             complement(5350..7404)
                     /gene="tkt"
                     /locus_tag="VFQ61_38410"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET9960425.1"
                     /translation="MSSLPTPILTQAANIARGLALDAVHKSQSGHLGLPLGCAEIGAV
                     LFGHTLRHSPDHPFWLNRDRFVLSAGHGSMFLYSWLHLSGYSGMSLEELKRFRQLGSN
                     TPGHPELTHTPAGVETTTGPLGQGIANAVGMAVSERMAAARFNTAKHKIFDHHVICLA
                     GDGCMQEGVALEALGFAGHHRLEKFILIYDQNSVTLDAMAAETQSEDTGKRLEATGFE
                     VQTVDGHDMAALEAALNHARSSQSGKPQAILARTVIGKGVPEVAGTQKAHGEGGAKFA
                     DAARRGLGLPEEHYYVAPEVRAFFDQRKRELEASYQAWLKTFDDWQAENPELAKQLHS
                     SRAYKFTQNEHNAPDENPNLVRDLLAAVPAFAEDQAIATRKAGQDVLQPLSRAVPLLI
                     GGSADLYGSTLNYIGDLKTGNDDFNPTHRTGRNIRFGIREHGMCSILNGIEAYGVFRA
                     SGATFLVFADYCRAAIRLAALSHLPVIYVFTHDSVGVGEDGPTHQPVETIPGLRVIPN
                     LDVIRPADPEETAGAYVAALERSEGPTLLALTRQNVPPLNQIPRDQRRDGVLKGGYVA
                     KRETAPLDLILLSAGSELQHAMKAAESLGPGTRVVSMPCFARFERQTPEYRESVLPRA
                     CRRRIAIEATVSSTWAPYVGLDGKIIGIDRFGMSAPGSQVMKELGMTAEHVVQAAKSL
                     QS"
     gene            7722..8825
                     /locus_tag="VFQ61_38415"
     CDS             7722..8825
                     /locus_tag="VFQ61_38415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960426.1"
                     /translation="MLPALALLASSVAFSGALARHSAIALSALGLLAWPSVCPALDSP
                     QAGSVPICVEYVAPPSCSQEAAFYAGVQGRTPRARTATPGEACLKVRVDIVPGATRVR
                     GELRVQDQTGRTDARSVEGAHCEEVVDALALTLALAIDPEATLVKTSDSTGASSNKPV
                     EAPASGAAAPKPEAPLNTPRTESPEPQELDLQSPLESATKWHLELGFEGLVANVLSVG
                     LSSGIGVGLRVRADRGPALGLSLEHMRNDFFGAPERAEMALTLARLSACPLQVSLGAL
                     ELEPCAAFMGGVLRVSGKAVAHPESVTRSWWSAGALGRGRFPLGATSAIELDLGATVP
                     FIRRKYVFRGPERAVAETPTLAPSASVRFIHRF"
     gene            9036..10019
                     /locus_tag="VFQ61_38420"
     CDS             9036..10019
                     /locus_tag="VFQ61_38420"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET9960427.1"
                     /translation="MLYRSLGRTGYQVSTISFGAWAIGGSWGPVEDGESMTTLQRALD
                     LGINFFDTADVYGDGRSERLLARLRKERSEPFVVATKVGRRSNPHVAGAYNRSNLTAY
                     VERCLGNLNVSALDLVQLHCPPTDVYYMPEVFGALDDLVAKGKIRHYGVSVERVEEAL
                     KAIEYPGVQSVQIVFNVFRQRPRDRFFALAQERKVGILARLPLSSGMLTGKMQPSTRF
                     ASDDHRAFNRQGAAFDRGETFSGVDYELGLRVVEEIKPYLKPGQTLAELALQFILSHE
                     AVTCAIPGARRPSQIEENARAGDLPALDAEILEALYALYDARIKPEVHQRW"
     gene            complement(10021..10905)
                     /locus_tag="VFQ61_38425"
     CDS             complement(10021..10905)
                     /locus_tag="VFQ61_38425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960428.1"
                     /translation="MTRWLCWIALASSFTACGGGSPPVQVSSSPRPGRYGAGGYATPS
                     VSSPYKGAFPSAPGAAPEVSRGQAYADESAAAERPGLGTEWGETRYSHVSNTAFDRSD
                     PSQPFALVTLRYNDRAGIQGALARLGSVSSVGLGAQAGGGGLTVRLLDEWGNPLRSYG
                     AGNQCYVEGSPGQRYVIEVQNRTGQRFEVVASVDGLDVLDGSAGTFEKRGYVMRPFST
                     LEIEGFRQSLDEVAAFRFGRVADSYAARTGDDRNVGVVGVAFFGEAGAPPPYLDSEQQ
                     RRDRAEPFPGRFAQPPRW"
BASE COUNT         1938 a         3546 c         3531 g         1968 t
ORIGIN      
        1 atcggcgtgg tggtggaagc cgccacgacc gatcaaatca tgaaggacgt ctcttccatc
       61 acgaaagacg tcaagcgcgt gaccgaggct ttggccgcca gcgtcggtac ggacacgggc
      121 cgggagaaca tcaaggacat cctggagaac ctcgctcgcg tcaccgagtc cctcaacaac
      181 accgtgcgcg agaaccgtga gtcatttcgc aacatcatgg tgaacgtcga gcgcattacc
      241 gccgaaggtc gacccgagtt gcgagagatt ctcgagaacg tgcgcgcctc gacggccgag
      301 gtccgcggtt tgctcgacaa gagcgacgag cagacggcgg gcgcgaaacc tggcgaagtg
      361 cggcagatca tcgaaaaggt caaccgcgcc agcacgaact tggaaagcgc gctcaagaat
      421 atcgacgagg tttcgggtcg gctcgagcga ggtgagggca cactcggccg attgacgaag
      481 gacgagaagc taatcgacga agttcaaggc gtggccgaaa ccgtcggaga cttcttgggt
      541 ggggtgtcac gtctgcaaac gatcttggtc ttgcgcacgg actaccaatt cctgaacagt
      601 accgtcaaaa gctacgtgga gctgcggctg caacctcgag aggacaagta ctacttgatc
      661 gaggtggtga acgatccgcg cggcttgact cgcttcgaac aggtggtggt ggatacggac
      721 aacccgaacg atccgccaca ctaccgcgag ctacggacgg ttacgaccaa cgccttccgc
      781 ttttctctgc agttcgcgca acgcttcggg cctttcaccg gtcgcttcgg gatcaaagag
      841 tccaccggtg gcgtcggcct cgatacctcg ctttttcaag atcgcttcga gctgcgacag
      901 gatctctacg gtttcggtga agtggtcttg ccgcgttggc gagtgtcgtt gggctatgag
      961 ttcatccacc gcctgtggct gctcggcggt gtggaagaca tcttgagcaa agcccggcgt
     1021 gactacttcg tcggcgcgca gctgaagttc acggacgagg atctcaagac aatcctgccg
     1081 ttcgcaccgg cgggcccgtg agcggtgggc ggcaacctat ttgggccact gaatccagct
     1141 gagtgcttgc gccgtcacca gagcggcgca ggcctccttc gacgtgatga tgcgggcgtc
     1201 gttgcggggg tgataagacg tgagccgctc gatgatgcgc tcgacgctca agcgctcgaa
     1261 caccagcgtc gataccaggt gcagcgaggt cggcgcggtg ccacgttctt tgcgattctt
     1321 ggtggcctcc gcgcgcaggc caggtaacac gatcgtggag ccgagcggca gcaacgcgcg
     1381 cggctcgccg tccgccgcca agatggcgcc cgccatcatg gggctcgcta gatccaaatc
     1441 gagcgggaaa tccacgcgcg tggcgcccgt gccggagtag aaggacacga gtccctcttg
     1501 ccacgtgcag agcgcggcca cgcgatcacg accctggtcg cggatcgcgc ggaagacatc
     1561 gacggcatcc accaatccca gaccaatcag cgtatccccc aagcgtccgc cgaagcgagc
     1621 caacgctccg agccccgtct cgagttgctc ccggctcagt tttccgcgtc ggaccaggta
     1681 ctctcccagc agctcctctc tctcggaaga cgcgacgtgg tgcaaacggc cctgctccag
     1741 atacatctct ttgcggctgg cgcggccgct cccgagcgtg cgctgcacga acagcgcgcc
     1801 actcatgccg cgggtcctca agtgcgcgag cacctcgagc atgctcgtgt cgcgcagcaa
     1861 caccgagtag tccggcgcgc ccggctcgaa gacacggctc gtggtggcgg tcgtggaggg
     1921 caataagtgt cgcgccaact cgtgaatgtc acggatgggc ttgagttcgg cgcccattag
     1981 cgccacctcg tcgtccccgc agagctcgcc cgtagccacc atctcgatca gcttggcgaa
     2041 gctgagggtt tcgacgtggt gcgcgccgga gcgtcgcagc cgcgacgcgg tttcggtggg
     2101 agccagcgtc tcgagcacgg gcgtggcgcg gtggtgaggg tgcccggtcg acttcaccgc
     2161 ttgcatccgg ctcacgctct cgcggatttg gccctccaac ttacgagcga actgcgaaga
     2221 atcgcgagcc caggtgaccc actccgagag cttgaccttg agatcgatgg gattcggacg
     2281 ttcgaacggc tccagcgcgc gcgccaggtc gtcggccgcg gcgtagcgat cgtcgggaga
     2341 gcgagccagc gccttctcga gcaccgcgaa cagtccgggg gggaagctac gggcggcgct
     2401 gcgcagcggc tcgatgttcg cgtcgcgaat ggcgagcagc accgcgagct ggccgctgcc
     2461 tgggaaaacg cgctcaccga gcagcatctc cccgagtagt gctgccagcg caaacaagtc
     2521 tgctcggtga tcgaaggcct cgcccgcgac ctgctctggc gcgagatacc cgaacttccc
     2581 cttcaaacct ccgggcgcag cgggctccga acgatcttcc gtacgcgcga tgccgaaatc
     2641 gccgagcttc acgtgcccct cgaccgacaa gtacacgttg gatggagtga cgtcgcggtg
     2701 cacgatgtag agcggcttgc cttcgcgatc caccgccgcg tggacggctg cgagcgcctg
     2761 caatacacgc cgcgcgacat agaccgcgag cccaggcgga atgcgccgct gctcggcctc
     2821 tgcacgccgt agcaagcgat acagatccac gccgtcgacg tactccattg ccaggtaagg
     2881 ttcgttgccg accattcccg caccgaaaac ggtcacgacg ttggcgtgcg cgacgcaacg
     2941 gtgcagctca gcctctcgct ccagaacgtc gaacccgctc tcgcgcgcgt tcggcagtaa
     3001 acgcttcacg accaagtgcg cggcgggcac ggtcccgacc ttggggcggg cgagaaacac
     3061 ctcggcactg ccgccaaccg cgatgcgtcg ctcgagcacg aacggcccga acggcttctg
     3121 cacgtccacg cgggcgccgt gtgattcagc cggcagagcc tcgctgggtt ggggctgact
     3181 gggttggggc tgactgggtt gcggctgact gggctgcggc tgagtcgctt gagcaaagtc
     3241 attccgcgcg gactcgctcg gcaggccttc cttccgctgc gcgtcgagcg gttggtctct
     3301 tggatgttgc tccggccgca cggggcacat tatggcgctc cttcgggccc ggaagcgagc
     3361 ccgagagcca acgactcggc gtaggcataa ccgctcaaac cgcggcgcca ttcccacccc
     3421 gaccagctcg aaaacggagc cttgcgccgc gacccttcgc accgggtcag gaaagtctgg
     3481 gcgaagcacg ccacattggg tccatacggc tcatcttggt ctgtcgaaag ccgtgctagg
     3541 aaactcgccc atgatcgtga agcggccgtc attattttgg cgaaaggcgc gctccctcgg
     3601 gagcgacgcg gctcacgcgg gtgcggtgcg gccgtcgctg cgtctcatct cggcactcgt
     3661 gttcgtcatt ctggcaagcg tggtgagctg tcgttgtcgg ccttcgcccg taaagagcga
     3721 aagcgctcaa gtcccttcgc tcagactcgt gctgctgtct tccgtcgcgg gcgccttgga
     3781 gccgtgcggg tgtgtgaagg atatgttggg cggcatcgat cacgcggcgg cctatctatc
     3841 gtcgagcaaa gggccaccga gcttggtgct cggtgccgga ccgatgttgt tcatggatcc
     3901 ggtgctgcac cccgagcgcc gcacgcaaga tgtgtggaaa gcggaaacca tgaatcgctc
     3961 gctcgccagc atgggcctca aagtctgggc ccctgcggtc aacgatttcg ccgccgggag
     4021 cgaagaattg gcgcgcatcg ccgcgctcgg cgcgcggccg ttatccgcga acctgaagag
     4081 ccagggtctc gcgctcgaag gccataaatt gtttcgagtc ggtggtgtgt ctgtgggcgt
     4141 ggctgcggca agcgccccta aacacctcgg caagtcgccg cccggcgtgg agctcggtga
     4201 agtcgagagt gcactccagc agcaagcccg agagctcgcg cagaaggggg cacagctccg
     4261 ggtcgtattg ctcgcgttgc cgcggggcga agccctgcgg gtcatcgaga aggtgccgga
     4321 ttttcagatc gcgttgatcg gcaaggccgt ggaccagggc gagtccaacg atcccgtcac
     4381 gccgaccacg cgcgtgggca acactctggt cgtcgagtcg cccaaccatc tgcaaggctt
     4441 ctacgtcgtc gacctgttct tgcgtgatgg caagttcgaa ctcgagaacg gcgatgcacg
     4501 aagcgacgaa cgcgcggcgc tcgaggttcg tatccgcgaa ctcgagcagc gcatcgcgca
     4561 ggcggaagca acgggcaacg tgcaacggag cgatctcgat gcgcgccgcg cggatctcac
     4621 caagctgcgc gaccagcgcg ctcggctcac gggctcttcg cgcccggctc acggcagcta
     4681 ctaccaggca cggctcgtgg aagtgcgcga gtccctcggt accgatccgg gtcagaccgc
     4741 ccaactctcc gagtattaca agcgcgtgaa cgagcacaac cgcgtggctt ttcgagatcg
     4801 cgtgcccgcc gcgccagcgg ccgacgagag cggttatgtc ggggtacaag tctgcggtgc
     4861 gtgccacacc caagcgctcg cggtttggca gaaaacgcgc cactcacgcg cgtacgaaac
     4921 tctggttgcc ggctccaagc agtacaactt ggactgcgtg ggctgccacg ttaccggtta
     4981 cgagaagccc gggggctcca ccgtcgcgca cgtgggtaac ttggcttcgg tgcagtgcga
     5041 ggtttgccat ggtcccggat cgcgccacat tgcgagcgtc tcggacaaga ctctcatcgt
     5101 cgccaaaccc gatccgggtt tatgcgcggc caactgccac catgaacctc acgtccaccc
     5161 ctcgtggaac gtcgcggctg cactccccaa gatcatccag cctggtcacg gcataagctt
     5221 agccaagtag gataggcgtt cgtgagcgct cactccccga gaacgtttcg gaagccgggg
     5281 tgatcgtcgg cgagcgctga cggctcgccg acgatccgtt tgcggcccga agccgctgcc
     5341 gcagtcagct cacgattgga ggctcttggc cgcttgcacg acgtgctcgg ccgtcatgcc
     5401 gagctccttc atgacttgcg agcccggtgc agacattccg aagcgatcga tgccgatgat
     5461 cttgccgtcg aggccaacgt acggcgccca cgtcgaagag acggtcgcct caatcgcgat
     5521 gcggcggcgg cacgcccgcg gcaacaccga ctcgcggtac tccggagtct gccgctcgaa
     5581 tcgcgcgaag cacggcatgc tcaccactcg cgtgcccggc cccaggcttt cggccgcctt
     5641 catggcatgc tgcagctcac tgcccgcaga gagcaggatc aagtccagcg gcgccgtctc
     5701 gcgcttggcg acatagccgc ccttcagcac tccgtcacgc cgctgatccc gcgggatttg
     5761 attgagcggc ggcacgttct ggcgggtcaa cgccagcaac gtgggcccct cgctgcgctc
     5821 gagcgcggct acgtacgcgc ccgccgtctc ttccggatcg gcggggcgga tcacatccag
     5881 gtttggaatc acccgcaatc cggggatggt ctcgacgggt tggtgggtgg gcccgtcttc
     5941 ccccacgccc acgctgtcgt gcgtgaacac gtagatcacc ggcaaatggc ttagcgccgc
     6001 caagcggatt gctgcgcgac aatagtccgc gaacaccagg aacgtcgcgc cgctcgcgcg
     6061 gaacacgccg taggcttcga tgccgttcag aatcgagcac atgccgtgct cgcggatgcc
     6121 gaagcgaatg ttgcgcccgg tgcgatgggt gggattgaag tcgtcgttac cggtcttcag
     6181 gtccccgatg tagttcagcg tcgagccata gagatccgcg ctaccgccaa tcagcagcgg
     6241 caccgcgcgc gacagcggtt gcaacacgtc ttgcccagct ttgcgcgtcg cgatcgcttg
     6301 gtcctcggcg aacgcgggaa cagcggcgag cagatcgcga accaggttcg gattctcatc
     6361 gggggcgttg tgctcgttct gggtgaactt gtacgcacgc gacgagtgga gctgcttggc
     6421 caattccggg ttctcggctt gccaatcgtc gaaagtcttc aaccaagcct gatagctggc
     6481 ctccagctca cgcttgcgct gatcgaagaa cgctcgcacc tcgggcgcga cgtaatagtg
     6541 ctcttctggc aaaccgagcc cgcgtcgcgc ggcgtccgcg aattttgcgc cgccttcccc
     6601 gtgcgccttc tgcgtgccgg caacctcggg cacgcccttg ccgatcacgg tgcgagccag
     6661 gatggcctgc ggctttccgc tctgcgagct acgcgcgtgg ttcagcgccg cttcgagcgc
     6721 ggccatgtcg tgcccgtcca cggtctgcac ctcgaagccg gtggcctcca gccgctttcc
     6781 cgtgtcttca ctttgggtct cggccgccat cgcatccaac gtcaccgagt tctgatcgta
     6841 gatcaggatg aacttctcga gtcgatggtg accagcgaag cccaaagctt cgagcgcgac
     6901 gccctcttgc atgcagccgt caccagccaa acaaatcacg tgatggtcga agatcttgtg
     6961 cttggccgtg ttgaatcggg ccgcggccat gcgctccgag acggccatgc cgaccgcgtt
     7021 ggcgatgcct tggccgagcg ggcccgtcgt cgtttctaca cccgccggcg tgtgcgtgag
     7081 ctcagggtgg cccggcgtgt tggaaccgag ctgccggaag cgcttcagtt cctccaagct
     7141 catgccggag tagccgctca aatgcagcca gctgtagagg aacatcgagc catgcccggc
     7201 agagagcacg aaccgatcgc ggttgagcca gaaggggtgg tctgggctat gtcgaagcgt
     7261 gtgcccgaac aacacggcac caatttcggc acagcccaga ggcaggccga ggtgcccact
     7321 ctggctcttg tgaacggcgt ccagcgcgag gcctcgggca atgttggcag cttgggtgag
     7381 tatgggcgtg gggagcgagc tcatgagttc atcgattcca ggattgggat gcggcaccgt
     7441 agctcgacgc gcgagtcgct ggaagccgga aattcccccg tggccaagct aatggcatga
     7501 cgattgcttc gaaacgggac gagcgcggga cgtgcacttc tcaggagcgt gcggctcctc
     7561 tcaagagtga cgacgagctg gttctcgggc gggccgaggc acgacgaata gggctcctcc
     7621 gagtggaggt cagttcgttg cgggcacgcg agtttgctcg aagctcagcc gaaccacccg
     7681 ccggtggaat attcaaagcg gaacatcgct catgaattcg gatgcttcca gcgctggcgc
     7741 tgctcgcgag ttccgtggca ttttccggag ccttggcacg tcacagcgca atcgcgctca
     7801 gtgcgctggg attactcgca tggccgagcg tctgtcccgc gctggattcc ccgcaagccg
     7861 gtagcgttcc gatttgtgtc gaatacgtag ctcctccgag ctgctcgcag gaggccgcat
     7921 tctacgcggg agttcagggg cgcacgccgc gcgcccgcac ggctacgccc ggcgaggcat
     7981 gcctcaaggt ccgcgtggac atcgtgccag gtgcaactcg ggtgcgcggt gagctccgtg
     8041 tccaagatca aacgggccgc acggacgccc gcagcgtcga gggcgcgcac tgcgaggagg
     8101 tggtggatgc gctggctctt actttggccc tggctatcga tcccgaagcc accctggtca
     8161 agacttccga ctcgaccggc gcatcgagca acaagcccgt cgaggcccct gcctccggtg
     8221 cggcagcacc caagccggaa gcgccgctga acacccctcg aacggagtcc cccgaaccgc
     8281 aggagctaga tctgcagagc ccgctggaga gtgcaactaa gtggcattta gagctcggct
     8341 tcgagggctt ggtagccaac gtcctgtcag tggggttgag ctccggtatc ggcgtggggc
     8401 tccgtgttcg cgccgatcgt ggtccggcgc tgggcttgtc tttggagcac atgcgcaatg
     8461 atttcttcgg cgctccggag cgcgcggaga tggcgctgac gctggctagg ctgagcgcct
     8521 gtccattgca ggtttcgctc ggtgcgctcg aactcgagcc ctgtgccgcg ttcatgggag
     8581 gggtgctccg ggtgagcgga aaagccgtcg cccatcctga gtccgtgacg cgttcctggt
     8641 ggagtgcggg ggcgctcggg cgcggtcggt ttccgctcgg ggccacctcc gcgattgagc
     8701 tcgacttggg ggcaacggtc cccttcatcc gccgtaagta cgtatttcgc gggccggagc
     8761 gagccgtcgc cgagacgccc acgctagcgc cctctgcctc cgtccgtttc attcaccgat
     8821 tctgagtagg ccagggaccc ggttctccgc cacctaagcg ggccaactcg gcttcgagat
     8881 acacttgccc ccctctagga cgcctattcc cgcggattga tttcgtgatc gagcggccag
     8941 gcaaaaatgt gccgcccggc agccggcagc tgaccaagaa aacgcgagac tggaacgctt
     9001 gttcgcgagc gcacctatca ctacccttcg cctccatgtt gtatcggtct ctcggtcgca
     9061 ctggctatca ggtgtcgacc attagctttg gcgcgtgggc tatcggaggg tcctggggtc
     9121 ccgtcgagga cggagagtcg atgacgactc tgcagcgcgc cctggatctc ggtatcaatt
     9181 tcttcgatac ggcggacgtc tacggcgacg gccgaagtga gcgcctgctc gcgcgccttc
     9241 ggaaagaacg ttcggagcca ttcgtggtcg caaccaaggt cggtcgacgc tcgaacccgc
     9301 acgtcgccgg tgcctacaac cgcagcaacc tcacggctta cgtagagcgg tgcctcggca
     9361 atctcaatgt ctccgcgctc gacttggtgc aactgcactg cccgccgacc gacgtctact
     9421 acatgccgga ggtgtttggc gcgctcgacg acctcgtcgc gaagggcaag attcgccact
     9481 acggcgtcag cgtcgagcgc gtggaagagg cgttgaaagc catcgagtac cctggggtac
     9541 agtcggttca gatcgtcttc aacgtattcc gacaacgccc gcgtgatcgc ttcttcgcgt
     9601 tggcgcagga gcgcaaggtc ggcatcttgg cgcgcctgcc gctcagctcc ggcatgctca
     9661 cgggtaagat gcagccgtct acccgcttcg cctccgacga ccaccgcgct ttcaatcgac
     9721 aaggtgcggc cttcgatcgc ggcgagactt tctccggtgt tgattatgag ctcggcttgc
     9781 gggtcgtcga agagatcaag ccctatctca agccggggca gaccttggcg gaactcgcgc
     9841 tgcagttcat cctcagtcac gaggcggtga cctgcgcgat tccgggagct cggcgtccga
     9901 gccaaatcga agagaacgcg cgtgcggggg acttgcccgc gttggacgcc gagatactcg
     9961 aggcgctgta cgcgctgtac gacgcgcgca tcaaaccgga agttcatcaa cgctggtgat
    10021 tcaccagcgc ggtggttgcg cgaagcgccc ggggaacggc tcggcgcgat cgcgccgctg
    10081 ctgctcggag tccaggtagg gagggggggc gccggcctcg ccgaagaagg cgaccccgac
    10141 cacgcccacg ttgcgatcgt cgccggtacg cgccgcgtag gaatccgcga ctcgaccaaa
    10201 acggaacgct gccacttcgt cgaggctttg gcggaagcct tcgatctcca gcgtagagaa
    10261 ggggcgcatc acgtaaccgc gtttctcgaa ggttccggcg ctgccgtcca ggacgtccag
    10321 gccgtcgacg ctggccacca cttcgaagcg ttggccagtt cgattctgca cctcgatcac
    10381 gtagcgctgc ccggggctgc cctcgacgta acattggttg cccgcgccgt agctacgcag
    10441 tgggttgccc cactcgtcaa gcagacgcac ggtcaagccc cctccgcccg cttgcgcgcc
    10501 gaggcctacg gaagacaccg agcctagtct cgcgagcgcg ccttggatgc ccgcgcggtc
    10561 gttgtaacgc aaagtcacga gcgcgaaggg ctggctcgga tcactgcgat cgaacgccgt
    10621 gttcgagacg tgactgtaac gcgtctcgcc ccattccgtt ccaagtccgg gccgttcggc
    10681 ggccgcgctc tcgtccgcat acgcttgccc tcgactcacc tcaggggcag cgcccggagc
    10741 gctcggaaac gcacctttgt acggggaaga tacgcttggc gttgcatagc cccccgcccc
    10801 gtagcgacca gggcgggggg acgaactcac ctgaacgggg ggcgagccac ccccgcaagc
    10861 cgtgaaagaa gaggcaagcg ctatccagca gagccagcgt gtcattcgat tatctcctgc
    10921 gacctttcgc ggaggacaac gccgagctct gctgccgctt acaccgatgt ttcgatcacc
    10981 tcg
//
LOCUS       DASTKU010001077        15055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1775653, whole genome shotgun sequence.
ACCESSION   DASTKU010001077 DASTKU010000000
VERSION     DASTKU010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 15055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15055
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775653"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VFQ61_38430"
     CDS             <1..497
                     /locus_tag="VFQ61_38430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET9960429.1"
                     /translation="SEQAQIDSALTRCPALRHVLRYDREFATWEDRLRPFRSSHVFLY
                     PTRYAGWGLVIPEAMAAGSVVLSTPEAEASAYFVRHRVNGIFLEPGVESLEREMRFCL
                     DNPAEVSEMGRRARLDAVDGHAPNVAQRVANALARLPNVRSRSARAEIAYRIASIVGR
                     VVPR"
     gene            complement(537..1493)
                     /locus_tag="VFQ61_38435"
     CDS             complement(537..1493)
                     /locus_tag="VFQ61_38435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET9960430.1"
                     /translation="MEKPRSVVPSSSAPRAPVARSASGARPCVEEPFIQAAEVWLPQG
                     TGLRHQSGFYGRHGSFARASFVTTFERGQGLPGTAFETARAQLCPSIQPDSPRNRRAL
                     DAGLESALALPVVREREVLAVVVFWAGRVGRSSLAIWNARGSPASCSDRCGGSGDVLR
                     VAQSMAERSAVSGLPVFEMPAQGTTGSTSASPPGGLALPNVGRRGSDEVITLVGSAAA
                     PWARAFELWLPNARGQLSLDHSYYGPGLSEFATASQGLNFEPGEGLPGRVYESGLPLV
                     FVGVHARPFVRAEAAEGAGLEYGIGIPFFEGAKVSAVLTWLG"
     gene            complement(1504..2502)
                     /locus_tag="VFQ61_38440"
     CDS             complement(1504..2502)
                     /locus_tag="VFQ61_38440"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET9960431.1"
                     /translation="MNRLSNVTLWRRVTQASPRPLTAPLFGLFWCALGMLGFSCTLTA
                     TRLAVTEMNGLFVGAGRAVVAAVLSALLLTYQRAPWPEPRHWRPLTLTALGVVIGFPT
                     LSAVALRSVPASHAQIFVGLSPLATALFASLRVNERPSRRFWYSASLGGCCVLIFGWV
                     HAGGQLAWADLLLLAAVALVGLGYAEGGQLAKELGGARVVCWALLVALPVSTPVALWG
                     LLHTRLDQITWRSSLGFAYVSVISMWLAFFAWYRGLAQGGVARASQIQLLMPVLGILW
                     AGILLGEHLDESTLLACAAVVASTGAHFVLMARNRPETRATSKASIGENASATPLA"
     gene            2504..3937
                     /locus_tag="VFQ61_38445"
     CDS             2504..3937
                     /locus_tag="VFQ61_38445"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET9960432.1"
                     /translation="MKSNSSSLRRQPPAPIAAQDLVHALLRSLVQSLGPGERLPSVRA
                     LVNEHHVSPVTVQRALAQLAREGHVITRPGDGTFVASRIAPARAIDVAWQSAALAAPF
                     ASARELESFFLLPREGTLVLGSGYPDPSLQPLELLAKAAQRASRRARTWSRLAVDGLP
                     ELRHWFANDVGGDAHADQALLVPGGQAALSTLFRALVPFGGPLIVESPTYFGALGIAR
                     AFGLRLIPVPHDAEGIRPDLLRAALASTGARVLYLQPWFSNPTGVSLSPERRSEVLKL
                     VKSANAFLIEDDYARDLVIDGAPPAPLYREGEGHVIYVRSLTKSTASGLRVGAILASG
                     PVLGRLKTARAVDDWFLSGLLQETALELVTSRSFAGHVRKLRAALKERRDAAVHALAR
                     HLPEARLVRLPTAGFSLWLELPSRVDETQLVEQAARASVHVNAGAPWFPAEPSGPHLR
                     ISIAGAPTDVLSEGIRRLGQIYRGARN"
     gene            complement(4016..6661)
                     /locus_tag="VFQ61_38450"
     CDS             complement(4016..6661)
                     /locus_tag="VFQ61_38450"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET9960433.1"
                     /translation="MSSSPLSSEPIPSPAAHANTGAAELHPGSIIADRYRIDELLGEG
                     NAGKVYLVEHVLLRKKLALKVLRRDLANVPEITARFEREAMAAANIEHPNIAGASDFG
                     RLLDGSVFLALEYVPGRSLRSELGAGRLSLERSLHIAIQIAAALSAAQELDIVHRDLK
                     PENVMLTARGNDSDFVKVLDFGIARVPMAETGSAPLTRAGVVFGTPEYMAPEQALGQR
                     ADGRADLYSLGVMLFEMIAGVRPFDAGATTLGQQVSAAPPPFSKRVPGLTVPIEVERV
                     VHKLMARERALRFATAEGALAALKDAKALLTPGESISPLVKAKKKPLAIPRVQPSSNV
                     GPNSQPPKAGRVVAPKPPSVQPPTKIAAPPPKLKASDAPSIRPLTNSPPPPGVTEDKP
                     HSNGVSVPPPLVRNPPFAANGAAAHGISVEHAPTEPPPPPGTEDTALPASPPPPPVRD
                     KASAPPAAAPNPPREKERLATFMPGDPLPAFDMTEPLPNVARPAIGSEPAPAPALPVS
                     SLPIPEAPTRLKLPNVERESLVEEVLEEDLRENFREPAGSLAQPNPSAPSRGGAWQDS
                     VVSAGRKAGDVFTSFASQVGLKTLSLHQQAVSAIEPRRSKLPGPLARLSANALLGIGA
                     GLVLLLVIGAVGLASRGSNKTAQNARTETELSSATTTSTPSAESPSLPEAPKADANPV
                     ASPAAQAAPSVASAPKSESQVLLDLAADYVAEHRDAEALGLVSRVLARDSALRDNPQV
                     AKVLAAAARSDVMRVEDESFALLQGPMGERGPEVLYELWLDKGARERVRHRAEDFLRS
                     KNFDRLSSAALYSAVKLRLARTCEQKRPLLDLAARVGGRETLVYLKELAARATCEPGS
                     QGECYACLSVDGRLNETIAKVEARL"
     gene            complement(6937..9666)
                     /gene="ppdK"
                     /locus_tag="VFQ61_38455"
     CDS             complement(6937..9666)
                     /gene="ppdK"
                     /locus_tag="VFQ61_38455"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET9960434.1"
                     /translation="MTRKLVYRFESGRAEGRAEWRDLLGGKGANLAEMANMGIPVPPG
                     FTIATEACVDALRTGQLPAGLWEAVRESMAFLEDRTGRKFGSAENPLLVSVRSGARSS
                     MPGMMDTILNLGLNDETMEGLGRQSQSPRFALDAYRRLLAMFADVVLGVEHALFEAPL
                     LAARRSIAKQKKLSIPDDPETLARLIPDSVLSADELREVVAAYKGIIREHAGKPFPND
                     PYQQLEAAIQAVFHSWNNPRAKLYRKMHGIPDEWGTAVNIQAMVFGNLGDTSATGVAF
                     TRDPSTGERRFFGEWLPNAQGEDVVAGIRTPRPLSRDQDSDGQSLQALMPDSYNELYA
                     IQGKLEHHFRDMQDIEFTIEAGKLYLLQTRNGKRSARASVRIAVDMVGEGLLHPDEAI
                     LRVDPERLSELLFPAINPKTSAVAVARGIAASPGAVGGKAVFNADDAEAYAARGEDVI
                     LIRLETSPEDLHGMKAAKGILTARGGATSHAAVVARGMGRPCVAGCSSLHVDYATGSA
                     SVHAANGDVCGNIRHGDFVTIDGSTGNLYLGRVDTVDATLGKEMETLLAWTERVSRMR
                     VRANADTKSQAEKALSFGAHGIGLCRTEHMFFDDERIAAVREMILSRDVTAREAALAK
                     LLPFQTEDFLDLFRVMKGKPVNIRLLDPPLHEFLPREKHQIQELAATMGVSVTDVERK
                     IAELFEFNPMLGHRGVRLGITFPEIPAMQVRAILEAACTVKQEGGDPHPEIMIPLAFS
                     SGELKAMRVVVDEVAEQVFTKRGVRVDFKFGTMIELPRAALLADELAKQAEFFSFGTN
                     DLTQTTLGVSRDDAGSFLPVYVERGILPDDPFVSLDKEGVGELVRIAVEKGRVARSEI
                     HFGVCGEHGGDPKSIEFCESLGLSYVSCSPFRVPIARVAAAQASLRRTRRYWR"
     gene            complement(9847..10440)
                     /locus_tag="VFQ61_38460"
     CDS             complement(9847..10440)
                     /locus_tag="VFQ61_38460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960435.1"
                     /translation="MQAPVRLRIASLALLGSACFGCGTSGQNWVLEPPEAFSSFERSA
                     RLSTGESPLLDPLPAQTAPSTAGSEPQRTKPRLSRVISLGESQSSAISAGPPRDSRND
                     GSPAFIIVNNYVGRPGSAPHAAGGVYSSSVYSAPFTGRNSSATTPGTHVLPSRSFDAR
                     THESRPTPNLGQDWSPPPNYGPLFPYKSSPAPVWGRR"
     gene            complement(10492..12378)
                     /locus_tag="VFQ61_38465"
     CDS             complement(10492..12378)
                     /locus_tag="VFQ61_38465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9960436.1"
                     /translation="MSAAATRLRPRVVGSELAFSVGSALLVALLTPYFLLLSGPVAVQ
                     GAGALSGGVAAGGFVGSAYAALRLIRYRYVLRALAVGTRAVEAEELHRLSEEPKRLLV
                     GWLLPTGVSIAATTIFLRPVLLDLNTGITLCLLGVVITAAASLPLFVLLRRAILGVLE
                     LGPHEAMREVVEEAEHRGLIGQRISRRMLAAVVTPVVCLSLGAALIVNAHVRRADERD
                     REETARVLARAALDASPSVVPRAGLEAAIEGGRALGFDAKRRDVNTDYAVVRGEDGVA
                     RLLAPLDSGSAEVRFNGSTVGFLSLSSLVVTLLAAAVAAFLGAEFGATLAHDLRGATR
                     NIRSLGTELVVSGSPSGARIVRSARFQVVVDLGRAIERLAQRFRVFAKAQERAIEARK
                     AAARMRGLFFASVSHDLKSPLNAILGFTQLVRRTAQLTPAQLESLDPIERRGHELLAL
                     IETILDAARVEAGQLELVLDPTEVTTLLNEAALKGRDLAAERDAPVVIEAAADLPILW
                     VDRVRMPRALATFIAHALRTTEAPMVRVTASLDGPRRLRISVDLDSSQFSLTRLESML
                     DPNRDPGVSEHRGFALGLRLARAVTELHGGKVEAKERKGGGGTLSAILPIQRESMPPP
                     SSVR"
     gene            complement(12375..13865)
                     /locus_tag="VFQ61_38470"
     CDS             complement(12375..13865)
                     /locus_tag="VFQ61_38470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET9960437.1"
                     /translation="MLKSKGKIPLLGLAPTLVVVVGFAAAFAIALLGTATLRRQSDLS
                     AQLRSRVLSVSLAARLSATPEEERVTLLERAARRSGAELLLVNQNGGIAAEAISGGKV
                     AGNIVDLLVRSEGESVTEFGRARFSASPLAPPNEHLSLIVLVSAPEKPFATGSLIASV
                     AALTALLVGAAALVALALTRDVDADVRFLRERIVQMAKEGSDPVGRHIPVRSVDQIGV
                     LTSAFNLLVDRFTAAEHAYRQDLAGALAYDRDRTAFLAALSHELRTPLNAILGFTDVL
                     LSEVDGPLTSDARENLNVVRSSGGHLRALIDDILDLSALESGELRLDCRPVDIYPVAA
                     EVAREMEVTARAKAIQLELRGSPATAFADPRRVRQIISNIVGNAVKFTEKGSVLLRVD
                     PRDGGAMISVSDTGPGIAADEREAIFQEYWQSRDAQRRRLGTGLGLAITRRLVQMHRG
                     FIDLKSQLGKGSTFTVVLPSRPPPKPAPKEEARPPLASEVDLGGAA"
     gene            complement(13884..14495)
                     /locus_tag="VFQ61_38475"
     CDS             complement(13884..14495)
                     /locus_tag="VFQ61_38475"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET9960438.1"
                     /translation="MMERQQGSSPMPQLLNIPQRAALRGAPIETPRTWLLPIEFNDGP
                     ELWDAVEGSRWHLERWLPWVPFNNTRDASMRYTEACALDWDAGRALRFAIRDRSQRSL
                     LGVVGLDSCVHLHRACELGYWLRRDVTGKGLMTEAARAAVEFAFSKVGIHRVRCAAAT
                     DNVASLRVIMRLGFRFEGIARQAEFVGSRWLDHALFARLSTDE"
     gene            complement(14492..>15055)
                     /locus_tag="VFQ61_38480"
     CDS             complement(14492..>15055)
                     /locus_tag="VFQ61_38480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960439.1"
                     /translation="VHKGRLHIAFAKDLGPQRVQVMLQTLALDDPALTTGLPVKAARD
                     TAGTVIGKMRPIGVPTGRNPEPSVACTDQGCFVAWDDEKAALTAFVDHERGPLWHRQF
                     GNKGARPALGRNERGAAVAWFEGSRLKLAPLTRDGVGKASVVSRVSGLQPYPDVMPGK
                     RPGEWYLAFRDYESAHLEVFALRAECP"
BASE COUNT         2591 a         4955 c         4916 g         2593 t
ORIGIN      
        1 gaagcgagca agcgcagatt gactcggccc tcacgcgctg ccccgctcta cggcatgtgc
       61 tgcgttacga ccgagagttc gcaacatggg aggaccgttt gcgtccgttc cgcagcagcc
      121 atgtgttctt gtacccaacg cgctatgcag gctgggggct cgtgattccc gaagcgatgg
      181 cggctggcag cgtagtgctg agcacgcccg aagccgaagc gtcggcgtat ttcgttcgtc
      241 accgcgtgaa cgggatcttc ctagaacccg gcgtcgagag cttggagcgc gaaatgcggt
      301 tctgtctgga caaccccgcg gaggtctccg aaatggggcg ccgcgctcgg ctggacgcgg
      361 tggacggaca tgcgccaaac gtcgcgcagc gtgtcgccaa tgcgctggcg cgcttaccca
      421 acgttcgctc gcgctcggcg cgcgcagaga tcgcctaccg cattgcgtcc atcgtcgggc
      481 gggtagtgcc ccgctaagcc tggcggagcg ctcgactggc gtagcggcgc tgagcgtcaa
      541 cctaaccaag tcaaaaccgc gctcaccttc gccccttcga agaaggggat gccgattccg
      601 tattcgagcc cggcgccttc cgcagcttcc gctcgcacga atgggcgtgc atgcaccccc
      661 acgaatacga ggggtaagcc cgattcataa acgcgcccgg gcaggccctc tcccggctcg
      721 aaattgagac cctggctcgc ggtcgcaaac tcactgaggc ccgggccgta gtaactgtga
      781 tcgaggctca gctgacctcg tgcattcggt agccacagct cgaacgcgcg agcccaaggg
      841 gctgctgccg atccaaccag cgtgatgacc tcatcgctcc ctctgcgacc gacgtttggc
      901 agcgcgaggc cgcccggcgg gcttgcagac gtgcttccgg tcgtgccctg agcgggcatt
      961 tcgaacacgg gtaaaccaga taccgcgctg cgctcggcca tactttgggc cacgcgcaac
     1021 acgtcgccac tgccgccgca gcgatcggag cagcttgctg ggcttccgcg cgcattccaa
     1081 atcgccaagc tgctgcggcc cacgcgcccc gcccagaaga ccactacggc cagcacttca
     1141 cgctctcgaa ccacgggtaa cgccagcgcg ctctccagtc cggcatctag agcacgcctg
     1201 ttcctcggcg aatccggctg gatgctggga cagagctggg cgcgcgcggt ctcgaacgct
     1261 gtgcccggga gcccttggcc gcgttcgaac gtcgtcacga agctggctcg cgcaaagctg
     1321 ccatgcctcc cgtaaaaacc tgattggtga cgcagaccgg tgccttgagg aagccaaacc
     1381 tcagcggctt gaatgaacgg ctcctcgacg caggggcgcg ctcccgacgc cgagcgagcg
     1441 acgggagcgc gaggcgcgct ggaggagggg actacggagc ggggcttctc cactcctgct
     1501 actttacgcg agcggcgttg cgctggcgtt ttctccgatg cttgccttcg atgttgcccg
     1561 cgtttcgggg cggttccggg ccatcagtac gaaatgagcg ccggtcgacg cgaccaccgc
     1621 cgcgcaggcg agtagcgttg actcgtccag gtgctctcca agcaggatgc ccgcccagag
     1681 tatgccgagc accggcatca gcagttgaat ttggctggct cgcgccacgc cgccctgcgc
     1741 gagacctcga taccaggcga agaacgcgag ccacatgctg atcacggaca cgtaggcaaa
     1801 accgaggctg ctacgccacg ttatctgatc gaggcgcgtg tgcagcaagc cccagagcgc
     1861 gaccggcgtg ctcacgggta gcgccacgag tagagcccaa cacaccaccc gcgccccgcc
     1921 tagttccttc gccaattggc caccttccgc atatcccaaa cccaccagcg ccacggctgc
     1981 gagcaacagt aagtccgccc aagccaattg gccgcccgcg tgcacccagc caaagatcag
     2041 aacgcagcaa ccgcccaagc tcgccgagta ccagaacctg cgtgacggac gctcgttcac
     2101 acgcaagctg gcgaagagcg cggtcgcgag cggggacaag cccacgaaga tctgcgcgtg
     2161 agacgccggc acactgcgga gggccaccgc ggagagcgtc ggaaagccaa tcacgacgcc
     2221 caaggccgtg agcgtgagcg gccgccaatg tcgcggttcg ggccagggtg cacgctgata
     2281 ggtgagcagc agggccgaga gcacggccgc aaccaccgcg cgccctgcac ccacgaacaa
     2341 accgttcatc tcggtaacgg cgagtcgggt cgcggtcaac gtgcagctga agcccaacat
     2401 gcccagagcg caccagaata agccaaagag cggcgccgtg agcggcctcg gtgaagcttg
     2461 cgttactcgc cgccaaagag taacgttact caatctattc atcgtgaaga gtaacagttc
     2521 ttccctccgg cgccagccgc cggctccaat tgcggcgcaa gatctggtcc acgccctgct
     2581 gcgatcgctg gttcaatcgc tcgggccagg cgaacgcctg ccgtccgtgc gcgccttggt
     2641 caacgagcac cacgtgagcc cggtaacggt gcagcgcgcc ttggcgcagc tggcgcgaga
     2701 aggtcatgtc ataactcggc ccggcgacgg taccttcgtc gcttcacgaa ttgcgccggc
     2761 ccgggccatc gacgtggcgt ggcagtccgc ggcgttggcc gcgcctttcg ccagtgcgcg
     2821 cgaactcgag tccttcttcc ttcttccgcg cgaaggcacg ttggtgctcg gcagcggcta
     2881 ccccgatccg tccttgcagc cgctggagct gctcgcgaag gcggcccagc gcgcgagccg
     2941 ccgcgcacgt acatggtcgc gcctggcggt ggatggtttg cccgaacttc gccactggtt
     3001 cgcaaacgac gtcggcgggg atgcgcacgc cgatcaggcc ctgttggtgc ccggaggaca
     3061 agcggcgctg tcgacattat ttcgagcgtt ggtgccgttc ggcgggcctt tgatcgtgga
     3121 gtctccgacg tacttcgggg cgctcgggat agcgcgtgct ttcgggctgc gcttgattcc
     3181 cgtgccgcac gacgcggagg gcattcgccc ggacttgctc agggcggcgc tagcctcgac
     3241 gggagcgcgg gtgctctatt tgcagccgtg gttctcgaac ccaaccggag tctcgctctc
     3301 tccggagcgg cgcagcgagg tattgaagct cgtgaagagc gccaacgcgt ttctgatcga
     3361 agatgattat gctcgggact tggtgatcga cggagcacct ccggcgcccc tgtatcgcga
     3421 gggcgagggg cacgtgattt acgtgcgttc gctgacgaag tcgacggcgt ccgggctgcg
     3481 cgtaggtgcg attctggcgt ccggaccagt gctaggccgc ttgaagacgg ctcgcgccgt
     3541 cgatgactgg ttcctctcgg gtttgctgca agagaccgca ctcgagttgg tgacttcgcg
     3601 cagctttgcg ggtcatgtgc ggaagttgcg agccgcgctg aaggaacggc gtgatgcggc
     3661 cgtgcacgcc ttggctcgcc acctgcccga ggctcgtttg gtgcggctgc ccacggccgg
     3721 cttctctctc tggctcgagt tgccctcacg agtcgatgag acgcagctcg tggagcaagc
     3781 cgcgcgcgcg tcggtccacg tcaacgccgg cgcgccttgg tttcctgcag agccgagcgg
     3841 gccgcacctg cggattagta ttgcgggtgc tccaaccgac gtgctcagcg agggcattcg
     3901 ccggctcggt cagatttacc gcggagcgcg caactgagcc gatcaggctc ggcgaacgag
     3961 cctgatccta cgccttagga gagacgctct cctccagagt ccgcgcgggg acccttcaga
     4021 ggcgcgcttc cactttggcg atggtctcgt tcagccgccc atccacgctc aggcacgcat
     4081 agcactcacc ctgcgagccg ggttcgcagg tggctcgggc cgccagctct ttcaagtaga
     4141 cgagcgtctc tcggccaccg acgcgagctg ccaaatcgag cagcggacgc ttctgttcgc
     4201 aagtcctcgc caagcgaagc ttcacggccg agtagagcgc cgccgacgac aatcgatcga
     4261 agttcttgct gcgcaggaag tcttcggcgc gatgtctcac gcgctcacgc gcgcccttgt
     4321 ccagccagag ttcgtacagc acttcagggc ctctttcgcc catggggcct tgcagtagcg
     4381 cgaagctctc atcctccacg cgcatcacgt ccgaacgcgc cgcggccgcc aacaccttgg
     4441 ccacttgcgg gttgtcgcgg agcgcactgt cgcgcgcgag cacgcggctg actaggccca
     4501 aggcttccgc gtcgcggtgc tcggcaacgt agtccgcggc caagtcgaga agaacctgcg
     4561 actcactctt tggcgcgctc gcgacgctgg gggcagcttg cgcagcgggg ctcgcgacgg
     4621 ggttcgcgtc ggctttgggc gcctcgggga gggaagggct ctcggcgctg ggggttgacg
     4681 tggtcgtggc actgctcagt tcggtttcgg tgcgcgcgtt ctgggcggtt ttgttggagc
     4741 cacgtgaggc gaggccaacc gcgccgatca ccagtagcaa caccaagcct gccccgatgc
     4801 cgagcagcgc gttggccgag agccgcgcga gcgggcctgg cagtttgctc cggcgcggtt
     4861 cgatcgccga gacggcttgc tggtgcaagc tcagcgtctt gagccctacc tggctcgcga
     4921 acgacgtgaa gacatctcca gccttgcgcc cggcgctcac caccgaatcc tgccaagcgc
     4981 caccgcgaga gggcgcgctc ggattgggct gagccaagct gcccgccggc tcgcgaaagt
     5041 tctcgcgcag gtcctcttcc agcacttcct cgaccaagct ctcgcgctcc acattgggca
     5101 gcttcaagcg cgttggcgct tcgggaatgg gcaagctgct cacgggtagc gctggcgccg
     5161 gcgccggctc cgagccgatg gcagggcgcg ccacattcgg tagcggctct gtcatatcga
     5221 acgcgggcag cggatcgccg ggcatgaaag tggcgagccg ctctttttca cggggaggat
     5281 taggagccgc ggctggtggc gcgctcgctt tgtcacgcac gggcggagga ggagggctcg
     5341 cggggagcgc ggtgtcctcc gtacccggtg gaggtggcgg ctcggtcggg gcgtgttcga
     5401 ctgagatgcc atgcgcggcc gctccgttgg cggcgaaggg cgggtttctc acgagggggg
     5461 gaggcacact caccccattc gaatgaggct tgtcctcggt aactccggga ggcggcggcg
     5521 agttcgtgag cggccttatg gatggggcat ccgaggcctt cagcttcggc ggaggtgcag
     5581 ctatcttggt cggcggttgc accgaaggcg gtttgggcgc caccacgcgt ccagccttgg
     5641 gcggctgaga attcgggccc acgttcgagc tcggttgcac tcgcggaatc gcgaggggct
     5701 tcttcttggc tttcacgagc gggctgatgc tttccccggg ggtgagcaac gccttggcgt
     5761 ctttcagcgc cgcgagcgcg ccttccgcgg tggcgaaccg gagcgcgcgc tcccgcgcca
     5821 tcaacttgtg gaccacgcgc tcgacttcga tcggcaccgt gagccctggc acgcgcttcg
     5881 aaaagggcgg cggcgccgcg ctcacctgct ggccaagtgt ggttgccccc gcatcgaacg
     5941 gccgcacgcc ggcgatcatc tcgaatagca tcacgccgag cgaatagaga tcggcgcggc
     6001 cgtcggcgcg ctgcccgagg gcttgttcgg gagccatgta ctcgggcgtt ccgaacacca
     6061 ctcctgcgcg cgtcagcggg gccgatccgg tctccgccat gggtaccctg gcaatgccga
     6121 agtccaggac tttgacgaaa tcgctgtcgt tgccgcgcgc cgtgagcatc acgttctcgg
     6181 gcttcaagtc ccggtgcacg atgtccagct cttgggcagc cgagagcgcg gccgcgattt
     6241 gaatcgcgat gtggaggcta cgttccaaag acagccggcc cgccccgagc tcactgcgca
     6301 agctgcgccc gggtacgtac tccaacgcca ggaacaccga cccgtccagc aagcgaccga
     6361 agtcgctggc tccggcgatg ttcgggtgct cgatattggc ggccgccatg gcttcgcgct
     6421 cgaagcgggc ggtgatctcc ggcacgttgg ccaggtcgcg ccgcagcacc ttcagggcaa
     6481 gcttcttgcg gagcagcacg tgctcgacga ggtacacctt gccggcgttg ccttcgccga
     6541 gcagttcgtc gatgcggtag cggtccgcga tgatgctacc tgggtgcagc tcggcggcgc
     6601 cggtgttggc atgtgctgca ggggacggga tgggttccga ggaaagggga gaggaggaca
     6661 aggctgccag gattctagtc tggttctcgg gacccgtccg gtgaggtttt gcaaccctgt
     6721 tcgctttctg acttggttcc tcgacgggcc tcatcgcttc taatggcgtg cgtgcgtccc
     6781 cccttttcgg gtgccttctt ttcgagacta gtgtgcagaa aagagaagag ggcctctttg
     6841 agaccctctt ccgctcggcc gcgtcgcgtt ccccgcgcgt tctcgctcta gtgctggctg
     6901 agaccgcgtg gcctcagcca gcatccgtag cgcgattcag cgccaatacc tacgggtgcg
     6961 ccgcagcgaa gcttgagcgg ccgcgacccg cgcgatgggg accctgaagg gtgaacaaga
     7021 cacgtaactt aggcccaaac tctcgcagaa ttcgatcgac ttggggtcac cgccgtgttc
     7081 accacagacc ccgaagtgaa tctcgcttcg agccactcgg cccttctcca ccgcaattcg
     7141 gaccagctcg ccaacgcctt ccttgtccag cgagacgaag gggtcgtcgg gcaagatgcc
     7201 ccgctctacg tacacgggga ggaagctccc cgcgtcgtcg cgcgagacac cgagtgtggt
     7261 ttgggttagg tcgttcgtgc cgaaggagaa aaattcggcc tgcttggcaa gctcgtcggc
     7321 caacaacgca gcgcgtggca gctcgatcat cgtgccgaac ttgaagtcga ctcgcacgcc
     7381 gcgcttggtg aacacctgct ccgcgacttc gtcgaccacg acccgcatcg ccttgagctc
     7441 acccgagctg aacgcgagcg ggatcatgat ctcggggtgg ggatcgcccc cttcctgttt
     7501 gaccgtgcac gcggcctcca agatagcgcg aacttgcatc gccgggatct cggggaacgt
     7561 gatgccgagc cgtacgccgc gatggccgag catggggttg aactcgaaca gctcagcaat
     7621 cttgcgctcc acgtcggtga cgctgacgcc catggtggcc gccaactctt ggatctgatg
     7681 cttctcgcgc ggcaggaact cgtggagcgg cggatcgagc agccggatgt tgaccggttt
     7741 gcccttcatg acgcggaaca aatccaagaa gtcttcggtc tggaacggca gcagcttggc
     7801 gagcgcggcc tcacgcgccg tgacgtcgcg cgagaggatc atctcgcgta cggcggcaat
     7861 gcgctcgtcg tcgaagaaca tgtgctccgt ccgacacagc ccgatgccgt gtgctccgaa
     7921 cgagagggcc ttctcggctt gtgacttggt gtcggcattc gcgcgaaccc gcatgcggct
     7981 cacgcgctcg gtccaagcga gcagggtttc catttctttg ccgagcgtcg cgtcgacggt
     8041 atcgacgcgg ccgaggtaca agttgccggt ggagccgtcg atggtgacga aatcaccgtg
     8101 gcggatgttt ccacagacat cgccgttcgc cgcgtgcacg ctggccgagc cagtcgcata
     8161 gtcgacgtgc agtgacgaac agccggcgac gcacggacgt cccatgccgc gcgcgaccac
     8221 cgcggcgtga ctcgtcgctc cgccgcgcgc cgtcaatatg cctttggcgg ccttcatgcc
     8281 gtgcagatct tcgggtgagg tctcgaggcg aatcaggatg acgtcttctc ctcgagcggc
     8341 ataggcttcc gcgtcgtcgg cgttgaacac ggccttgcca cccaccgcgc cgggggacgc
     8401 cgcgatgccg cgtgcgaccg cgacggcgga ggtcttggga ttgatcgcgg ggaagagcag
     8461 ctcggagagt cgctccggat cgacgcgcag aattgcttcg tccggatgca gcaatccctc
     8521 gccgaccata tccaccgcga tgcgcaccga cgctcgcgca gaccgcttcc cgttgcgggt
     8581 ctgcagtaga tagagcttac cggcctcgat cgtaaactcg atgtcctgca tgtcgcggaa
     8641 gtgatgctcg agcttgcctt gaatcgcgta cagctcgttg tagctgtcgg gcatcaaggc
     8701 ctgcagagac tgaccatcgg agtcctgatc gcgggacagc gggcgcggcg tgcggatacc
     8761 cgctacgacg tcttcacctt gcgcgttcgg cagccactcg ccgaagaagc gccgttcgcc
     8821 cgtgctggga tcgcgcgtga acgcgacgcc ggttgcgctg gtgtcaccca agttgccgaa
     8881 caccatggcc tggatgttca ccgccgttcc ccactcatcg gggatgccgt gcatcttgcg
     8941 gtagagcttg gcgcgcgggt tgttccagct gtggaatacc gcctggatcg cggcttcgag
     9001 ctgttgatag ggatcgttcg ggaacggctt gccggcgtgt tcgcggatga tgcccttgta
     9061 cgcggcaacc acttcccgca gttcgtcggc gctcagcacc gagtccggga tcaagcgggc
     9121 caaggtttcc gggtcatccg gaatggagag cttcttctgc ttggcgatgc tgcgccgagc
     9181 agccaacagt ggcgcttcga acagcgcgtg ttcgactccc aacacgacgt cggcgaacat
     9241 cgccagtagg cgtcggtatg catcgagcgc gaagcgcggc gactggcttt gccgacccag
     9301 tccttccatc gtttcgtcgt tcaagcccag attcaggatc gtatccatca tcccaggcat
     9361 cgagctgcgt gcgcccgaac gaacactcac gagtagcgga ttttctgctg aaccgaattt
     9421 gcgcccagtg cgatcctcca agaacgccat gctttcacgc acggcctccc acagcccggc
     9481 gggcagttgg cccgtccgca gcgcgtcgac acacgcttct gtggcgatag tgaagccagg
     9541 cggcacggga atgcccatgt tcgccatctc agccaaattg gcccctttgc cgccgagcag
     9601 atcacgccat tcggcgcgac cttcggcgcg acccgattcg aatcggtaca cgagctttct
     9661 cgtcatgggc ggcccccggt cgcaagggct gtgccgtcca tcggacaaac atcacgtcgg
     9721 acggaatatc tccgccgtag cgtcgagcgc gatcagcggc gctttacggc gtgagtaatc
     9781 cgcgcaagac ctgcgttgcc cctctggtgc gtcgcaaggc ttgcgatgct gctcgatgat
     9841 cgggagtcag cggcgacccc aaaccggtgc cgggctcgac ttgtagggaa acaacggacc
     9901 gtagttgggt ggcggggacc agtcttgacc gaggttgggc gtgggccgcg actcgtgcgt
     9961 tcgtgcgtcg aagcttcgcg agggaagaac gtgcgtgcct ggcgtagtcg ctgacgagtt
    10021 gcgacctgta aaaggcgcgg agtagacgct gctggagtaa acgccgcccg cggcgtgcgg
    10081 tgcgcttccg ggccgcccca cgtagttatt gacgattatg aaagccgggg agccgtcatt
    10141 tcgcgaatcg cgcggagggc ccgcggaaat tgcgctgctc tggctctcac ccagtgagat
    10201 gacccgactg agtcggggtt tcgtgcgctg cggctcggag cccgccgtcg agggagcggt
    10261 ttgcgcaggg agcggatcga gcagcggcga ttcgccagtg gagagacgtg cagagcgttc
    10321 gaagctcgag aacgcttcgg gcggttcgag tacccaattt tgcccactcg ttccgcagcc
    10381 gaagcatgca gaaccgagca gcgccaggga agcgatccga agccgcacag gagcttgcat
    10441 gtcccgaagt atgcgctcga tcacccgaac gcgcgacctg cgctttgcct gctagcgaac
    10501 ggagctcggc gggggcatgg attcgcgttg aatggggagg atggcggaca aggtgccgcc
    10561 gccgcccttg cgttccttgg cttcgacttt gccgccgtgg agctccgtta cggcgcgcgc
    10621 caggcgcaaa cccaacgcga aaccgcggtg ctcgctcacg ccgggatctc ggttcggatc
    10681 gagcatggac tctagtctcg tcaggctgaa ttggctcgaa tccagatcca ccgagatacg
    10741 cagtcgccgc ggcccatcca agctggccgt gacgcggacc atgggagcct cggtggtgcg
    10801 cagggcatgc gctatgaagg tggcgagcgc gcgcggcatg cggacccgat cgacccacaa
    10861 gatcggcaaa tctgcggccg cttcgatcac caccggcgcg tctcgctctg cggccaaatc
    10921 tcggcctttt agcgcggcct cgttgagcag cgtggtgact tcggtgggat ccaacaccaa
    10981 ctcgagttgc cccgcttcga cccgcgcggc gtcgagaatg gtttcgatca acgcgagcag
    11041 ctcgtgcccg cgtcgctcga tcggatccaa gctctccagt tgagccgggg tgagctgcgc
    11101 cgtgcgtcgc acgagctggg tgaaacctag gatggcgttg agcgggctct tcaaatcgtg
    11161 gctcacgctc gcgaagaaca agcctcgcat gcgggcggcc gctttgcgcg cctcgatcgc
    11221 tcgttcttgc gctttcgcaa agacgcggaa gcgttgcgcc agccgctcga tggcgcggcc
    11281 cagatcgacc accacttgga agcgtgccga gcgcacgatg cgggcgccgc tcgggctgcc
    11341 actcaccacg agttcggtcc cgagcgagcg aatgttgcgg gttgcgcccc gaagatcgtg
    11401 cgcgagcgtg gctccgaatt cggcgccgag aaacgcggca accgccgccg ccaacagcgt
    11461 aacgaccaga gacgacagac tcaggaagcc gaccgtcgaa ccgttgaagc gcacctccgc
    11521 gctgcccgag tccagcggag caagcagacg agcgacgccg tcttcgccgc gaacgacggc
    11581 atagtccgta ttcacgtccc ggcgcttggc atcgaagccg agcgcgcggc cgccctcgat
    11641 cgcggcctcc agcccggctc gtggcaccac cgaaggagag gcatctaggg cggcccgcgc
    11701 gagcacgcgc gcggtctcct cgcgatcacg ctcgtccgcg cgtcgcacgt gcgcgttgac
    11761 gatcagcgcg gcgccgagcg agaggcaaac tacgggcgtg accaccgcgg cgagcatgcg
    11821 acgggatatg cgctgcccaa tcaggccacg gtgctcggcc tcttcgacta cctcgcgcat
    11881 cgcctcgtgt gggccaagct ccagtacccc caagatggct cgccgcagta aaacgaacag
    11941 aggcagggaa gccgcggcgg taatcacgac ccccaacaag cacaacgtga tcccggtgtt
    12001 caaatcgagc agcaccggtc gcaggaaaat cgtggtcgcc gcgatcgaca cccccgtcgg
    12061 cagtagccag cccacgagca gccgcttcgg ctcttcgctg aggcgatgca gctcttcggc
    12121 ttcgacggca cgcgtgccca ccgccagcgc gcgtaaaacg tagcggtaac ggatcaagcg
    12181 cagcgccgca taggcactgc ccacgaaacc cccggccgca acaccgccgg acaatgcacc
    12241 ggcaccttgg accgcaaccg gaccgctcag cagcaaaaaa taaggggtca gcagcgccac
    12301 gagcagcgcc gaacccacgg agaacgccag ctccgagccg acgacgcgtg gcctcagccg
    12361 ggtcgcggcc gcactcacgc ggcacctccc aaatcgactt ccgatgccaa gggcgggcga
    12421 gcctcttcct tgggtgcggg tttcggcggc ggtcgcgacg gtagcaccac cgtaaacgtg
    12481 gagcccttac cgagctggct cttgaggtcg atgaagccgc ggtgcatttg cacgagccga
    12541 cgcgtgatcg ctaggccgag acctgtcccg aggcgtcggc gttgagcatc acgggattgc
    12601 caatactctt gaaagatagc ttcgcgctcg tcggcggcga tgccggggcc cgtatccgaa
    12661 accgagatca ttgcgccacc gtcgcgcgga tccactcgca gcagcacgct gcccttttcg
    12721 gtgaacttga ccgcgttgcc gacgatgttg ctgatgatct gtcgtactcg tcggggatcc
    12781 gcgaaggcgg tggcggggct accacgcagc tcgagctgaa tggctttggc ccgagccgta
    12841 acctccatct cgcgcgcgac ttccgcggcc acggggtaga tatcgaccgg tctgcagtcg
    12901 agccgcagct cccctgactc gagcgcggaa agatccagaa tgtcgtcgat caacgcgcgc
    12961 aggtggccac cgctcgagcg caccacgttc aggttctcgc gtgcgtcgct ggtcaacggt
    13021 ccatccactt cgctcagcag cacgtccgtg aagccgagga tcgcgttcaa gggcgttcgg
    13081 agctcgtggc tcaacgccgc gaggaaggcc gtgcgatcgc ggtcgtaagc caacgcgccc
    13141 gccaaatcct gtcggtaggc gtgctcggca gccgtgaaac gatcgacgag caggttgaag
    13201 gcggacgtca gcacgccaat ctggtccacc gagcgcaccg ggatgtggcg acccaccgga
    13261 tcggagcctt ccttcgccat ctgcacgatg cgctcgcgca ggaagcgtac gtccgcgtcg
    13321 acgtctcgag tcaacgccag cgcgaccagc gcggccgcgc cgacgagcag tgcggtgagc
    13381 gccgccaccg aagcaattag agagcctgtc gcaaacggct tctcgggtgc gctcaccagg
    13441 acgatgagcg agaggtgttc attgggaggc gccagcggcg acgcgctgaa gcgggcacgc
    13501 ccgaactcgg tgacgctctc gccctcgctc cgtaccagca ggtcgacgat gttcccggcg
    13561 accttaccgc ccgagatagc ctccgcggcg atgcctccgt tctggttgac caagagcagc
    13621 tcggcgcccg agcggcgggc ggctcgctcg agcagcgtga cgcgctcttc ctcgggggtc
    13681 gcgctgaggc gcgcggcgag cgagacggag agcacacggc ttcggagttg cgcggagaga
    13741 tcactctgcc ggcgcaacgt cgccgtgccg agcaacgcga tcgcgaaggc ggcggcgaag
    13801 ccaacgacca ccacgagggt gggcgccaag cccaacaacg gaatcttacc tttgcttttc
    13861 agcacggaaa ccgagcgacg agtctactcg tcggtggata gacgcgcgaa tagcgcgtga
    13921 tcgagccaac gcgaacccac gaactcggct tggcgggcga tcccctcgaa gcggaagccg
    13981 agccgcatga tgaccctcag actcgcgacg ttgtccgtcg cggccgcgca gcgcacgcgg
    14041 tgaattccca ccttggagaa cgcgaactct acggccgctc gtgccgcctc ggtcatcagc
    14101 cccttgccgg tcacgtcgcg tcggagccag tagcctagct cacacgcgcg gtgcaaatgc
    14161 acacaggaat ccagcccgac cacgcccaat aaactgcgct gactgcgatc gcggattgcg
    14221 aaccgcagcg cgcgccccgc atcccaatcg agggcgcagg cttcggtgta gcgcatactg
    14281 gcgtcccgcg tgttgttgaa gggcacccat ggcaaccaac gctcgagatg ccagcgtgag
    14341 ccctccaccg catcccacag ctcggggccg tcattgaatt cgatcggcag cagccaagtc
    14401 cggggagtct cgataggagc accgcgcagc gcggcgcgct gcggaatatt cagaagttgg
    14461 ggcattggcg acgaaccctg ctgacgctcc atcatggaca ctccgcgcgc agcgcgaaga
    14521 cttccaggtg ggccgactcg taatctcgga aagccaggta ccattcgcct ggcctcttac
    14581 cgggcatcac atccgggtac ggctgcaggc cgctgacgcg gctcaccacg ctcgccttgc
    14641 ccacgccgtc gcgggtcagc ggcgcgagtt tcagacgcga gccttcgaac catgcgacgg
    14701 ccgcgccacg ctcgttgcgc ccgagcgcgg gacgcgcccc cttgttaccg aattgccggt
    14761 gccaaagcgg cccgcgttcg tggtcgacga aagccgtgag cgcggctttc tcgtcgtccc
    14821 aagccacgaa gcagccttga tcggtgcacg ctacgctcgg ttcggggttg cggcccgttg
    14881 gcactccgat cggtcgcatc ttgccgatta cggtgccggc ggtgtcgcgc gctgctttca
    14941 cgggtaagcc cgtcgtcaac gctggatcat cgagggccag cgtttgcagc atcacctgta
    15001 cgcgctgcgg ccccaggtct ttggcgaacg cgatgtgcag tctacccttg tgcac
//
LOCUS       DASTKU010001078         6118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1776201, whole genome shotgun sequence.
ACCESSION   DASTKU010001078 DASTKU010000000
VERSION     DASTKU010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776201"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFQ61_38485"
     CDS             complement(<1..451)
                     /locus_tag="VFQ61_38485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960440.1"
                     /translation="MIDVTDFDVQSWVITPAAKLAGDTSKVQQWLVVLTGVANINWKA
                     GALLNGQEGWQDQNYRILMDWQSPTYWAKGQPSPPVEHFFQAEQWAPYATFNSVLAAH
                     HGDTFAGFAVNTWRMGLVERRAKDGSLYRNIVRGLEIDVSVRDSSLIA"
     gene            1021..2841
                     /locus_tag="VFQ61_38490"
     CDS             1021..2841
                     /locus_tag="VFQ61_38490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HET9960441.1"
                     /translation="MAVTLAAGCASEVTEDGPGNPATSAGANGSGAGAGAGNNGSNSG
                     NPGGSTGPGGTTAPGAGNSTGNPTPGGGSNGAGGTPGGAGSTSANCTPNVPGTSQIPR
                     LTNDQYDRTVRDLLGVTTVTALGNAVPSTRLATDQAGGMTALAWANYKDVGEKIAAQV
                     MADPKLKANFLKCTPTAGDTTCLHNTITEFGRRAFRRPLTQAEVARFDKIVSAGATIT
                     PSGKPEEVAEVLLNMFLISPSFLQRAEIAEVSDNAGHFTLTAHEIASRLSYMLWGTMP
                     DAALSQAADQGQLSSLDQVATQARRMLQDPKAREMVNKFHQAYLVMRAEGRWGKAQRD
                     PSLFPSFKPEMVPTLTEETLRIFDKLAFTPGATFRDFFLTPVAFVNKSTAPLYGLDAS
                     KYGTELTEVTLDANQRPGFLTRLGFLINFSSYTRTNPIYRGAFISKEVLGIAIPAPPP
                     GAADTPLPSGADLTTNRKQVDAQTSGANCIGCHHTFINPPGFVMETFDAVGAIRTKDG
                     NQPLDTKADVTFDVGASAATISSPAELMARIAASPGAMRQYAKKWVGFAFERENDPAD
                     ECTVNQLAAKMANNGYAVLDLVTDITQTQVFRVRAVEVSQ"
     gene            2838..4478
                     /locus_tag="VFQ61_38495"
     CDS             2838..4478
                     /locus_tag="VFQ61_38495"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HET9960442.1"
                     /translation="MNRVNPPAINRRFFLKGVAGACVAAPFLSSVNEKLAKAQGMPAS
                     KPRRLVMMYTHNGCLTTRWFPQKEDGALQASDLTGTYLEVMAPYVSKLLLPRGFRSLN
                     GYAVGQSIDPHDQACGSKLTCAFISDDKNRYATAESLDHTIAKQINPGDKKPMVLGVG
                     AASTLIKEVISFSGPNTAFPTNVNPQSVYTLLSGVVAQGGMSTGGGMGTTSGGTGTTT
                     GMTRADYLVAKGKSVIDLVSDDLQTYKRLNMSKADQSRIDAWLSLLRETETGVMNPAV
                     SAACNAMAGTNLGISDASLTDASPKGPITAGNVLGPRGSAEGDANLATSFTKGGDMMM
                     NLIALSMMCDVNRVFLLVYPGYVVFNWDGIKHTKEHHGLSHRTGDFSVGGNCFAGVVD
                     MIAQIDKWYASKFARLVKTFNDIQEGDGTLLDNTATMWLPELSDGAAHNLNNLPILIA
                     GSCGGYLAQGKAVNVEGRNIGRGNSESSCQNGGTIGNTGSTGGNVPINKLYVTLMNAV
                     GCKGADGGPVTTFGKFDGSKEADGIKNPGEVASLKAAK"
     gene            complement(4596..5426)
                     /locus_tag="VFQ61_38500"
     CDS             complement(4596..5426)
                     /locus_tag="VFQ61_38500"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HET9960443.1"
                     /translation="MHLHRDASTGQEQVFLKAPIFGEPWQNEVAAAGASTVRGFLGAQ
                     PSRERLIALTHKVMASTSQLAEGFLARAPAGTVACKAGCDHCCYQSVGVTPPEAIAIF
                     EFVKQTRTGAAFEAFAAHVAERYTQTRGLSSSERFSPQHPCVFLESGRCSIYEVRPLS
                     CRGMNSLNAEECATRLRDPEARAAFLLNGAGSHSFLEPIRAFHALSAGLQLGLAELYA
                     LDVRPLDLIAAMQLLLRDSESVVRDWMSGAPAFESTLGSDSSGSAAIRRLSGALSADE
                     "
     gene            complement(5680..>6118)
                     /locus_tag="VFQ61_38505"
     CDS             complement(5680..>6118)
                     /locus_tag="VFQ61_38505"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET9960444.1"
                     /translation="PLNTFQANDLYPMSVVAADFTSDGVPDVVTANIGRNTLSLMAST
                     DDGLLADPKELGGGANPFPLVAGDLNGDCRPDLVTGNAYSEVWVLMGLGAGRFQTEQR
                     FQVDARPGALALADMNRDGKLDIVGVRGQANEVFVLYSMTASP"
BASE COUNT         1189 a         1908 c         1919 g         1102 t
ORIGIN      
        1 ccgcaatcag cgaagaatcc cgaacgctga cgtcgatttc tagtcccctg acgatattcc
       61 gatagagtga gccgtccttg gcgcggcgct ctaccaggcc catgcgccac gtgttgaccg
      121 caaagcccgc gaatgtgtca ccgtggtgcg ccgcgagcac cgagttgaag gtggcgtacg
      181 gagcccactg ctccgcttgg aagaagtgct ccacgggggg agaaggctgc cctttggccc
      241 agtaggttgg gctctgccag tccatcagga tgcggtagtt ctggtcttgc cagccctctt
      301 gtccgttgag tagggcccct gctttccagt tgatgttggc gacgccagtc aacaccacca
      361 gccattgttg gactttgctg gtgtcacccg cgagcttcgc ggccggagtg atcacccaac
      421 tctgcacatc gaaatcggta acatcgatca tggatagccc ctcggcacga gcggggagtt
      481 gctctaccgg gcggggatct gcatcgaggg tgccatgggt tctgcccgca attcgacgac
      541 cgttcgaagg agctgccagc agcgcttgcg cagagcagga ctcgacacgc aatgttctcg
      601 cccttgttcg ggagttgcgc gtcaacggcg tggcgcgacg gagcgtcggg ttttgcctgg
      661 tctcagcccc ggcgcgccgt tctgctgtcg aagctcgtat gtggagtccg caaccttcta
      721 gcggagcgga tccagccgtc tccagtgggt gcatcggccc aaccggccgt ccgcgcgtgg
      781 tcgagatcgg ggccgcgtct tcgttggatt attgaagaca cacagcattt tcgttttgta
      841 ttggaaacag ccgaccctaa atctgtggct tcggaatcag ctgtggggaa tgggcgactt
      901 gaagcaaggc cagagtccga ctcgcgttta ccggagtctg gtttaccggg gtctgtccgc
      961 ttgaaaaccg cgagcagctg cgaggtcagg gcgatgtaca tccgtcggag cgcattatta
     1021 atggcagtca cgctggcagc gggctgcgct tcagaagtca cagaggatgg gccgggcaat
     1081 ccagccacgt ccgctggagc gaacggatcg ggtgcaggcg ctggcgctgg caacaatggc
     1141 agcaattccg ggaatcccgg cggttcgacg ggccctggtg gcacgactgc accgggcgct
     1201 ggtaacagca cgggcaatcc tactccgggc ggaggaagca atggcgcggg cggcacgccg
     1261 ggcggcgcgg gctcgacgtc ggcgaattgt acgccgaacg ttcctggcac atcgcaaatt
     1321 ccgaggctca ccaatgatca gtacgaccgc accgtgcgtg atctgctcgg agtgacgacc
     1381 gtgactgcgt tgggtaacgc ggtgccttcg acacgcctcg ccaccgatca agccggcggg
     1441 atgacggcgc tcgcctgggc caactacaag gacgtgggcg agaagatcgc ggctcaggtg
     1501 atggccgacc ccaagctgaa ggcgaacttt ctcaagtgta cgcccaccgc gggcgacacc
     1561 acatgcctac acaacacgat taccgagttt gggcgccgcg cgttccgtcg gcccctcacg
     1621 caggccgaag tagcgcgatt cgataagatc gtttccgccg gcgccacgat cactccgagc
     1681 ggtaagccgg aagaggtggc cgaggttctg ctgaacatgt tcctgatctc gccgtccttc
     1741 ctgcaacgcg cggagatcgc ggaggtgtcg gacaacgcgg ggcacttcac gctcacggcg
     1801 cacgagatcg cctcgcgtct ctcctacatg ctgtggggca cgatgcccga tgcggccctc
     1861 agccaggctg cagatcaagg gcagctgagc tcgctcgatc aggtcgccac gcaggcgcgg
     1921 cgcatgctcc aagatcccaa ggcgcgggag atggtgaaca agttccacca ggcttatctg
     1981 gtgatgcgcg ccgaagggcg ttggggcaag gcgcagcggg atccttcgtt gttccccagt
     2041 ttcaagcccg agatggtgcc gacgctcacg gaggagacgc tccgcatctt cgacaaactc
     2101 gccttcactc ccggcgcgac cttccgtgac ttctttttga cgccagtcgc gttcgtcaac
     2161 aagagcacgg ccccgctcta cggcctggac gcgagcaagt acggcacgga gctcaccgag
     2221 gtcacgttgg atgccaatca acgtccgggc tttttgacgc gcctcgggtt cctcatcaac
     2281 ttttcctcct acacccgcac gaacccgatc taccgcggcg cattcatcag caaggaagtg
     2341 ctcggaatcg caatacccgc gccgcccccc ggagcggcgg atacgccgct gccatctggt
     2401 gctgatctga ccaccaatcg caaacaggtg gacgcgcaga cctcgggcgc caactgcatc
     2461 ggctgccacc acactttcat caacccgcct ggtttcgtca tggaaacctt cgacgccgtc
     2521 ggtgcgatcc gcacgaagga tggcaatcag ccgctcgata ccaaggcaga cgtgacgttc
     2581 gacgtgggcg cgagcgccgc aaccatcagc tcccccgccg agctgatggc acgcatcgcc
     2641 gcttctccgg gggccatgcg gcagtacgcc aagaagtggg tcgggttcgc gttcgagcgt
     2701 gagaacgacc ccgccgacga gtgcaccgtg aatcagctgg ccgcgaagat ggccaacaac
     2761 ggctatgccg tgctcgatct tgttaccgat attacgcaga cccaggtgtt ccgggtccga
     2821 gctgtcgagg tttcccaatg aaccgcgtga acccgcctgc catcaaccgc agattcttcc
     2881 tcaagggcgt tgcaggcgcc tgcgttgctg ctccttttct gagctcggtc aacgagaagc
     2941 tagccaaagc gcagggcatg cctgcgagca aacctcgccg gctggtgatg atgtacacgc
     3001 acaacggctg tctgaccacg cgctggttcc cgcagaaaga agacggcgcg ctgcaggctt
     3061 ccgatttgac gggcacttac ctcgaggtga tggcgcccta cgtgagcaag ttgctgctgc
     3121 cacgcggctt tcgttccttg aacggctacg ccgtcggaca aagcatcgat ccccatgatc
     3181 aagcctgtgg ctccaagttg acgtgcgcat tcatcagcga tgacaagaac cgctacgcca
     3241 cggccgaatc gctcgatcac acgatcgcca agcagatcaa ccccggtgac aagaagccga
     3301 tggtgctcgg ggttggcgcg gcgagcacgc tgatcaagga agtgatctcg ttctcgggtc
     3361 cgaacaccgc gttcccgacc aatgtgaacc cacagagcgt gtacaccttg ctctcggggg
     3421 tcgtcgctca aggcggtatg tccaccggcg gggggatggg cactacgtcc ggcgggacag
     3481 gcacgaccac gggcatgacg cgcgccgact acctcgtcgc gaaaggcaag agcgtcatcg
     3541 acttggtttc tgacgatctg caaacctaca agcgactgaa catgagcaag gcggaccagt
     3601 cgcgtatcga cgcctggttg tctttgttgc gcgagacgga gacgggcgtg atgaaccctg
     3661 ccgtttctgc ggcctgcaac gccatggccg gcacgaatct cggaatcagc gacgcgtcgt
     3721 tgaccgacgc cagccccaaa ggcccgatta ccgcgggcaa cgtgcttgga ccgagaggca
     3781 gcgccgaagg ggacgccaac ctcgcgacct cgtttaccaa gggcggcgac atgatgatga
     3841 atctgatcgc gctgtccatg atgtgcgatg tcaatcgcgt gttcttgttg gtttacccgg
     3901 gttatgtggt gttcaactgg gatggcatca agcacaccaa agagcatcac ggcctgtcgc
     3961 accgtaccgg tgatttctcg gtgggaggca attgcttcgc cggcgtagtg gacatgatcg
     4021 cccagattga caagtggtac gcgagcaagt tcgcgcgcct ggtcaagacg ttcaatgaca
     4081 tccaagaggg tgatggcacg ttactcgaca acaccgccac gatgtggctc ccggagctct
     4141 cggacggcgc cgcccacaac ctcaacaatc tgccgatctt gatcgccggc agctgcggcg
     4201 gatatctcgc gcaaggcaag gccgtcaacg tcgagggacg gaacatcggg cgcggcaaca
     4261 gcgagagttc ttgtcaaaac ggcggcacca tcggcaacac ggggtcaacg ggcggcaacg
     4321 tgccaatcaa caagctctac gtcacgctga tgaacgcggt gggttgtaaa ggtgcagacg
     4381 gtgggcccgt cacgaccttt ggcaagttcg acggcagcaa agaggccgac gggatcaaga
     4441 acccgggcga ggtcgcctca ctcaaggccg ccaagtaggc ccgttcgtca gccgccccgc
     4501 gaagccctcg ataaccgagg gcatcgcgct ttcgtggcat tgagcccctc tgaatgagag
     4561 caccggagcg cccatggcaa gggcgctttg gccgtctact cgtccgccga cagcgcgccg
     4621 ctcaaccgtc gaatggccgc gctaccagag ctatcgctgc caagcgtcga ctcgaaggcg
     4681 ggagccccgc tcatccagtc gcgaacgaca gattctgagt cgcggagcaa cagctgcatg
     4741 gccgcgatga gatccagcgg acgcacgtcc agcgcgtaca gctcggcaag cccgagctgg
     4801 agaccggcgc tcaaggcgtg gaacgcgcga atcggctcca gaaacgagtg gctgcccgca
     4861 ccgtttagta agaacgcagc acgcgcttcg ggatcgcgta agcgagtggc gcattcttcc
     4921 gcgttcagcg agttcatgcc gcgacaggat aggggccgca cctcgtaaat cgagcagcgc
     4981 ccggactcga ggaagacgca cggatgttgc ggtgagaagc gctccgatga ggacagcccg
     5041 cgcgtctgag tgtagcgctc ggccacgtgc gccgcgaagg cctcgaacgc ggcccccgtg
     5101 cgcgtctgct tcacgaactc gaagatggcg atggcctccg gcggagtgac acctacggat
     5161 tgatagcagc agtgatcaca gccggccttg caggccaccg tgcccgccgg cgcacgcgcc
     5221 aaaaacccct cggcgagttg cgacgtcgac gccatcacct tgtgcgtcag cgcgatcaac
     5281 cgctcacggc tcggctgagc gccgagaaaa ccgcgcaccg tgctcgcgcc cgctgcagcc
     5341 acctcgttct gccagggctc gccgaagatg ggggccttta gaaatacctg ctcctgcccg
     5401 gtcgaggcat cccggtgcaa gtgcaagtgt acgcgtccag cagcatcgcg gccaaagcct
     5461 gctcgagcgc tggatggctc gcggacgctt cgttcccgct gcttctgtct ctgtcgatct
     5521 tttctttttt gtttgttcgt cactgagacg ctaagacgct gagacgctga gacgcgcgga
     5581 gtttggggac gcctgcgagg gtaccaccag atgcattccc gtcgaggccg agcgggctcg
     5641 acgggaagtc tggatttctc gaaattcagg gctgaggcac tagggggagg cggtcatgga
     5701 gtacagcacg aagacttcgt tggcttggcc ccggacgccg acgatatcga gcttgccgtc
     5761 tcggttcata tcggcgagcg ccagcgcgcc cgggcgtgcg tcgacctgaa agcgttgctc
     5821 ggtttgaaac cggcccgcac cgaggcccat cagtacccag acctcactgt aggcgttgcc
     5881 ggtgaccaga tcgggccggc aatcgccgtt caagtctccc gccacgagcg ggaatggatt
     5941 tgcgccaccg ccgagttctt tggggtctgc cagcaatcca tcatcggtgc tggccatcag
     6001 agacaaggtg tttcgtccga tgttggccgt gacgacgtcg ggcacaccgt cgctcgtgaa
     6061 gtctgccgcg accaccgaca tggggtaaag gtcgttggcc tgaaacgtat tcaagggt
//
LOCUS       DASTKU010001079        14402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_2001951, whole genome shotgun sequence.
ACCESSION   DASTKU010001079 DASTKU010000000
VERSION     DASTKU010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 14402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14402
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001951"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VFQ61_38510"
     CDS             <1..712
                     /locus_tag="VFQ61_38510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960445.1"
                     /translation="SLGPPVQRLLGTGSYMSPRLREIAAPSGATEAASDEGEILAVVS
                     FGRVPVKIAQRVPIGLALTYASGALSPTDASRANYLAGQGLVTWVNYPELEPSRSSFG
                     SPSFSLDGSSVALESPTAVDEEARRAWDDAKGTVVAAAITRMLSRVVAGEAVRQTAKD
                     GLVGALLGLGVQATLTAADTPDTRSWQTLPARMVFGRARVKAGQHEVVLTSRGVKKRE
                     TVTVKPGGFAVVAHTVLF"
     gene            complement(723..2453)
                     /locus_tag="VFQ61_38515"
     CDS             complement(723..2453)
                     /locus_tag="VFQ61_38515"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET9960446.1"
                     /translation="MYQKVHAIRPTTLTALAQGTLVAAFLLASASTSAQPADAQSGAA
                     PAVSTTASGGFEEGLRAELDSYIQNALTEFDVPGAAVAILKDGKVAYRGAFGQRGDQH
                     PAPVDSQTLFMIGSVTKSMTATMTGTLVDDGILSWDAPVKQYLPTFALAVPEYSESVT
                     LRHLLSHQSGVSRRDIALFLAADRPLEMLQAVSTLPVHSAPGQSYEYQNQMYALSGLI
                     TARAAGARLTNQSLEKTYERLMQTRLFDRVGMPRTTLDFDRAMHSPNHAWPNEFSPLE
                     GRVTHVQPSFENFATGVAAAGGVWSNIDDMARYALMQVSEGKNPEGRRVISEAALEET
                     HTQMVPGEQGGYGLGWGVALTPFGKLIQHSGGTAGFGCDVALSPEHDWGIVVLTNRAS
                     STWFVQAVENYAASVLLGIERRDDAELLNAEAAFRAQISQLVALTVPASRAEVAQYLG
                     AYEGGISVGLRGSDLIIRTSLGELQARPVPAYPGGFVMVGNVLSGAVAYFASDEAGNM
                     TLTLGALATEGEEVSILQPETRAKLPPGTRPQRPRGFPQVFQDGWREMARQLHARGVH
                     WHKHHPELFR"
     gene            2674..4734
                     /locus_tag="VFQ61_38520"
     CDS             2674..4734
                     /locus_tag="VFQ61_38520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960447.1"
                     /translation="MVYGAMRRARVGLGQKPKESPGNFPHARHRCTRSHWAGAARAVS
                     EAVYGISITACAISTLLVTPTVALAQTGRALVAEPVQGEKIRIDGDLREWPSKMTPLA
                     ETLQGSSPPEARTVVGYDDKNVYLALRVHDKNIARTAQASDAEDHATLTLAFPRGRLE
                     YTTYEVDVFPGKPGKLPGVVKLRGKPLAGAKAVENPTERELLIEAQIPWASFPEAARV
                     RVGLRGSVSYTNADNPGTVKAICSTSTGRSGKGLGPLLLEAEQGLADLVREKGLSDLP
                     ARETYGNVAGDGTQERVAVYGPYITISGPGFRGGKEFYFAEIGASSADMIRGLELSDF
                     DGDGRDEIVIRKRVGSKDKYREMLDVMKVGKDDAPFVAFSHEIAIKTPDGSITNKVKI
                     ANRAITVEQGQSDGFEADSYAEPLPGGNVHSALLPWESVGSRTFKWQGNGFVKSDEQT
                     ESPKLSKKEGGKKHRSDANRPAEPPPPRPPTADEMLDRVYALYKKERSASGRPRFDFV
                     TDVAADSSPERVVVHGKDIVVFGKNFRSGASYAFIQMGVADEKDVIDATARDLTGDGK
                     AEIVVRAVLRAKASKALGGDTVERHALLVYGIKEERLARVFAAETGRALGKNQILGVV
                     AFEPASRGTRIELRPGRAIGWTENSYPFPPDTTTAGGLEPLLLPWSGNKRAYHYNGSE
                     YVLE"
     gene            complement(4886..5704)
                     /locus_tag="VFQ61_38525"
     CDS             complement(4886..5704)
                     /locus_tag="VFQ61_38525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HET9960448.1"
                     /translation="MGIFSRLAALIKSNINDLISRSEDPEKMLNQIVVDMSEQLLEAR
                     KQVAASIADEKRLEKQAAEEGGRAAEWERRAMLALRAGDENLAKEALARKKEHETLRD
                     QFLEQWQKQKNAVEQLKLALRALNNKIEEAKRKKNLLIARKKRAEAQKAIAETMSGLK
                     NASAFEAFDRMASNIERMEAEAEASAELHEQYTGDALAHKFSELEVAHGVDDDLLALK
                     RKMGLAPPEPAPVPQAAVRVEGAPGALEAAEQEELARALAELDAEQEAPLRVKR"
     gene            complement(5770..6183)
                     /locus_tag="VFQ61_38530"
     CDS             complement(5770..6183)
                     /locus_tag="VFQ61_38530"
                     /inference="COORDINATES: protein motif:HMM:NF017719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CesT family type III secretion system chaperone"
                     /protein_id="HET9960449.1"
                     /translation="MIQSSEDLTAYLTRLERRFERLENGTFVLTMGANRPLVALRLEP
                     PLLVVRVEMGRAPAEAESSQPLFKRLLELNVSSLLHAAYGLQNGNVVLSAALPLESLD
                     LAELEGVLADVDLAVAEHVPALSELLHRAHDASAS"
     gene            complement(6243..7121)
                     /locus_tag="VFQ61_38535"
     CDS             complement(6243..7121)
                     /locus_tag="VFQ61_38535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET9960450.1"
                     /translation="MFLALVLTAGFMFVEAIVGFVSGSLALIADAGHMLADAAALGLA
                     LSAQRFANRPRTERSTFGFRRAEVLAAFVNGMALSGVAVLILKESVERWLVPHSIDGR
                     SVAVTATAGLAINIAVAAILMRGHKHNLNVRAALAHVLTDALGSVAAIISGLCVWLWG
                     VYRADPLLSAGIALLVFVSGWRVIKETVGILLESAPPHLDVAAIEKTILQCPGVSEVH
                     DLHVWRISDQFDALTAHVVLSRGSHGTEVCRMVAARLKSVHGLEHVTIQPESPRPDEV
                     VRLRRSRDGDPLRHAG"
     gene            7140..7469
                     /locus_tag="VFQ61_38540"
     CDS             7140..7469
                     /locus_tag="VFQ61_38540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960451.1"
                     /translation="MGVRMVVVVSVLVLVSMLVLVLVSMLVSMLVLVLVSMLVFGVVL
                     IRVPVRYALSTPRFSAPMRTMLVISLVMRRVVVRSVVVRTSRPVLVSMLMRVAMIVLV
                     SMAVPEL"
     gene            7867..9318
                     /gene="guaB"
                     /locus_tag="VFQ61_38545"
     CDS             7867..9318
                     /gene="guaB"
                     /locus_tag="VFQ61_38545"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET9960452.1"
                     /translation="MFDRNARECLTFDDVMLVPAFSDVVPSEVDVRSRFTRGIELNVP
                     IVSAAMDSVTESRTAIAMARQGGIGIIHKNLTIRDQAQEVDRVKRAESGIILDPVTVR
                     PSQSLREALAVMRAHDVSGVPVVEGEHPVGILTARDIRFEKNLDQPVSALMTTELVTV
                     PPGVATEQARELLHQHRIEKLLVVDNGKLVGLITIKDLLQAERNPLAVKDDRGRLRVG
                     AAIGPGSDRLQRTEALVEVGVDVIVIDTAHGHSSRVIEAARDTKKRYPNVQLVAGNIA
                     TAEAALALIDAGVDAVKVGIGPGSICTTRIIAGIGVPQVTAIADCVEVGDRHDVPVIA
                     DGGIKYSGDVTKAIAAGASSVMIGSLFAGTDEAPGELVLYQGRSYKVYRGMGSIGAMK
                     RGSKDRYGQAGTADEKLVPEGIEGRVPHRGSLASIIAQLVGGLRSGMGYTGSLTVQDL
                     RKNAQFIRITGSGLRESHVHDVIITEEAPNYHV"
     gene            complement(9502..10407)
                     /locus_tag="VFQ61_38550"
     CDS             complement(9502..10407)
                     /locus_tag="VFQ61_38550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960453.1"
                     /translation="MPIPRAGFEIARAVRKKPLHVRRAVSAAILSGALLGTSISTGSC
                     TGSRHDAEAGAAPPSAARNGPLGIVGMKGQPLGVTDAPSGHRAAPAAPSAAPTAMPNA
                     ASNSSGPSGSPARAPSVAESARVASAPSGSPNAGGPTSEPALLGADGSPLPQTDERPR
                     ADSASFVARLAALVRAIELDDPSLAHGSFFPLVAYQQVKAIEKPERDYRFRLLTAFDR
                     DIHDLHKTLGKDATGIRFERIEIPENQARWMKPGQEGNRLPYFRVLRSRMFVKTAQGA
                     ERALVLTSLISWRGEWYVVHLNGFK"
     gene            10623..11582
                     /locus_tag="VFQ61_38555"
     CDS             10623..11582
                     /locus_tag="VFQ61_38555"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HET9960454.1"
                     /translation="MAREKMAQAVSRRALLRTALSASALLSSALGRVAPANAAKYSAA
                     DGELEQRLLELPQAQGSPHKARLLLPKRITGELPLLILCHGLGESNDVALGLRAWTEL
                     YGLLTAVERLAHPPVERLFPKLNYLPEQRLLELNRALAEQPLSPCAMVCPFTPNPYVR
                     GGDSMDRIERFADYVESSLIPAVRKEAPIAKTAAGVGLAGVSMGGYVALELAVRRPTS
                     FGRVSSVQGAFGAPALGRYTEGLLNAQRTGALRDLLLLTSRDDPFRAAHERLARALSN
                     STLRHELIVRPGPHNQPWLREVGCLELLCWNDRALRSAAPSPT"
     gene            complement(11647..12693)
                     /locus_tag="VFQ61_38560"
     CDS             complement(11647..12693)
                     /locus_tag="VFQ61_38560"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HET9960455.1"
                     /translation="MSQKIRVMIVDDSVVVRKMLEHTLNAQPDMQVVGMAADPVEARE
                     VLLRSHPDVMTLDVEMPKLDGLSFLRALMKHQPMPVVMVSAVTERGSQNALAAMDAGA
                     VEIIPKPHGSFSVDAVKKEIVEAIRRAAKAPVRQRQSGAKTNAARAQPQHFQGHQIVA
                     IASSTGGTQALTRVLSQLPKNTPPLVIVQHIQAAFCASFAARLKKDTELDVREGSAGE
                     VLLPGMVRVAPPDIHMIVEPVAHGGFRTVLRNGPRVCFQRPAGDVLLTSVARAAARAA
                     VGVVLTGMGADGAEGLLAMRTAGARTIAQDEASSVVYGMPREAVQLGAAQHVVPLERM
                     AQTIVELLVKPRAA"
     gene            complement(12690..13121)
                     /locus_tag="VFQ61_38565"
     CDS             complement(12690..13121)
                     /locus_tag="VFQ61_38565"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HET9960456.1"
                     /translation="MEAKLHEALSESLIEVLETLAYESPAACDPHASTDWLRADLEFS
                     GGVQGEFSLSIESGEARSLAAALLGQMPGDLTDDDALAAVGEIANMVCGGCVSRVAPN
                     ALVQLAAPRAQRSSESETRDALWLEGEAGHLSAHIEMSRPS"
     gene            complement(13122..14006)
                     /locus_tag="VFQ61_38570"
     CDS             complement(13122..14006)
                     /locus_tag="VFQ61_38570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9960457.1"
                     /translation="MSVVATRMIEHTVGIGELAVSREPTSTIVTHSLGSCIGVTAWDA
                     QSRAAGMLHFQLPDSSLDLSKAQKMPALYGDTGIHVLVNELAKLGANPRRLAVRLYGG
                     ANMFQGTAAFDIGHRNHLAARRALWKCGILVEHEDVGGTSSRTLRLEVGTGKVLVKTW
                     EVEKTARARGVNLLLVDDSPTMRRLLARAIESSNLVVEKIVEAENGASALDVLRRSWV
                     DLVVTDLNMPVMTGPELLRRMRAHAGLRRVPVVVVSTDGTEARRQELEALGVKGFVKK
                     PFRPEDVVRTLSSALGVR"
     gene            complement(14006..>14402)
                     /locus_tag="VFQ61_38575"
     CDS             complement(14006..>14402)
                     /locus_tag="VFQ61_38575"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET9960458.1"
                     /translation="SRRALERAKRAIYPMQEIERMPRERVRRWFERGVDQFDGYARVN
                     RNITSMVRFEYLNLLSDPLPTTRFEVVFLRNVMIYFDAETRQRLLDRVVERLAPGAWL
                     FIGSSETLSEVVTPLELVSPSIYHLPGEG"
BASE COUNT         2621 a         4614 c         4605 g         2562 t
ORIGIN      
        1 gtcgcttggg ccgccggtgc agcgactgtt gggcacgggt tcttacatga gcccgcgctt
       61 gcgcgagatc gcggcgccga gtggcgcgac cgaggcggcg agtgacgagg gcgagattct
      121 cgcggtggtg agcttcggcc gcgtgccggt gaagatcgcg cagcgtgtac cgattgggct
      181 cgcgctgacg tatgcgtccg gcgccctgag cccgactgat gcaagccgcg cgaactacct
      241 cgcggggcaa gggctcgtga cgtgggtgaa ctatcccgag ctcgagccca gccgtagctc
      301 gtttggaagc ccgagtttca gcttggacgg gagttcggtc gcgctcgaga gccccaccgc
      361 agtcgacgaa gaagcccgtc gggcttggga tgacgccaag ggcacggtcg tcgctgccgc
      421 catcacgcgc atgctgtctc gagtggtcgc gggcgaagcg gttcgtcaaa cggccaagga
      481 cggcctagtg ggcgcgctgc tcggcctcgg agtgcaggcc acgctcacgg ctgcagacac
      541 gccggacacg cgtagttggc agacgttgcc cgcaagaatg gtattcggtc gggcccgcgt
      601 gaaggctggc caacacgagg tcgtgttgac cagccggggt gtgaagaagc gcgaaaccgt
      661 gacggtcaag ccgggcggct tcgccgtggt ggctcacacg gtgctgttct gagccgaaga
      721 gctcagcgga acagctccgg gtgatgtttg tgccagtgca cgccacgcgc atgcagctga
      781 cgggccattt cgcgccagcc atcttggaag acctgcggga acccgcgcgg acgctgaggg
      841 cgtgtaccgg gcgggagctt cgcgcgcgtt tccggctgga gaatgctcac ctcctcccct
      901 tcggtagcga gcgcgccaag ggtcagcgtc atgttgcccg cttcgtcgct cgcgaagtaa
      961 gccaccgcac ccgacagcac attacccacc atcacgaatc cgcccggata agctggcact
     1021 gggcgcgctt gcagctcacc caagctcgtc ctgatgatta agtcagaacc gcggagcccc
     1081 acactgatgc cgccctcgta agctccgaga tattgcgcaa cttctgctct cgaagccggt
     1141 accgtgagcg cgacgagctg cgaaatctgg gcacggaagg ccgcttccgc attcaggagc
     1201 tccgcgtcat ctcgacgttc gatcccgagc aagacggaag ccgcgtagtt ctccaccgcc
     1261 tggacgaacc aagtgctcga cgcacgattg gtgagcacga cgatgcccca atcgtgttcc
     1321 ggggatagcg ccacgtcaca accgaagccg gcggtgccac cgctgtgttg aatgagctta
     1381 ccgaacggag tcagagccac gccccacccc aggccgtagc ctccttgctc accgggtacc
     1441 atttgcgtgt gggtctcctc gagagccgcc tccgatatga cgcgtcggcc ttcgggattt
     1501 ttgccctcac tcacctgcat caaggcgtac cgggccatgt catcgatgtt ggaccaaacg
     1561 cctccggccg cggccactcc ggtagcgaag ttctcgaagc tcggttgtac gtgggtcacg
     1621 cgcccttcga gcgggctgaa ctcgtttggc caggcgtggt tgggcgagtg catggcgcgg
     1681 tcgaagtcga gcgtggttcg cggcatgcct acccggtcga acaaccgcgt ctgcatcagt
     1741 cgctcgtaag ttttctccag gctttgattc gtgagccgtg cccctgcggc gcgcgcggtg
     1801 atcaagcccg atagagcgta catctggttc tggtactcgt agctctggcc gggcgcagag
     1861 tgcactggca gcgtggacac cgcctggagc atctccaatg gtctgtcggc ggcgaggaac
     1921 agagcaatat cccgacggga gactccgctt tgatgactga gcaggtggcg cagagtcacg
     1981 ctttcggagt actcgggcac ggccaacgcg aacgtaggca aatattgctt caccggcgcg
     2041 tcccaactca aaataccatc gtccacgagc gtgccggtca tggtcgccgt catcgacttc
     2101 gtcacggagc cgatcatgaa cagagtctga gagtccaccg gtgcaggatg ctggtcaccg
     2161 cgctgaccga acgcccccct gtaagcaacc ttgccgtctt tcaagatcgc gactgcagcc
     2221 ccaggcacat cgaattcggt gagcgcattt tggatatagc tatcgagttc ggcgcggagc
     2281 ccctcctcga agcccccgct cgcagtggtc gaaaccgcgg gtgcggctcc tgattgagcg
     2341 tctgcgggct gagcgctggt ggacgcgctc gcgagcagaa acgcggcgac gagcgtgccc
     2401 tgcgcaagcg ccgtcaaggt cgtgggacgg atagcgtgta ctttctgata catgttttct
     2461 cctcacctcg gcgaactggc gttcacaccc cttccggcag aacgacgttt ccgactgcgg
     2521 aggcaacgct gaagcacgcg ccgtgcccac gcggatggag cacttcgctg taaaacgctc
     2581 gcagcaaaca cgcgctgcgg gagctcccaa ctcggctgcg cggagtcgat aggcgtcccg
     2641 tgaacgcggt cgccgcgcag gaagcgagca gtcatggtat acggcgccat gcgcagagcg
     2701 cgagttggtt tagggcaaaa accaaaagaa tcacccggca atttcccgca cgcgaggcat
     2761 cgatgcaccc gtagtcattg ggccggcgcc gcgcgcgcgg tctccgaagc cgtctacgga
     2821 atctcgatca ctgcctgtgc gatctcgacg ctattggtga cgcccacggt cgcgctcgcg
     2881 cagaccgggc gggcgctggt ggccgaaccc gtgcagggcg aaaagatccg catcgacggt
     2941 gatctgcggg aatggccgtc gaagatgact ccgctcgcgg aaacgcttca gggctcttcg
     3001 cctccggagg cgcgaacggt cgtagggtac gacgataaga atgtttatct ggcgttgcgc
     3061 gttcacgaca agaacatcgc gcgcacggct caagcttccg acgccgaaga ccacgcgacc
     3121 ctgacgctcg catttccgcg cgggcgcttg gaatacacca cgtacgaggt ggacgtgttc
     3181 ccgggtaagc caggtaaatt gcccggcgtc gtcaagctgc gcggcaagcc gctggctggg
     3241 gccaaagccg tcgagaaccc gaccgagcgt gagctcctga tcgaggcgca gattccgtgg
     3301 gcgagctttc cggaagcggc gagggtgcgg gtgggattgc gcggctcggt gagttatacg
     3361 aacgcggaca atcccggcac ggtgaaggca atctgctcga cgagcacggg ccgaagcggc
     3421 aaaggtctag gccccctgct gctcgaggcc gagcaagggc tcgcggattt ggtaagggaa
     3481 aaaggcctct ccgatctgcc cgcgcgtgag acttacggca acgtggcggg cgatggtacc
     3541 caggagcgtg tagccgttta cggtccctat atcacgatca gcgggcctgg cttccgcggc
     3601 ggcaaggagt tctatttcgc agagatcggc gcgagcagcg cggacatgat tcgcggcctc
     3661 gagctcagcg atttcgatgg cgacgggcgc gacgaaatcg tgattcgcaa gcgcgtgggc
     3721 agcaaggaca agtatcgcga gatgttggac gtgatgaagg tgggcaagga cgatgctccg
     3781 ttcgtcgcct tctcgcacga aatcgcgatc aagacgcccg acggctccat caccaacaag
     3841 gtgaagatcg cgaatcgggc gatcacggtc gagcagggcc agtccgacgg tttcgaagcg
     3901 gacagctatg cggagccgct gccgggtggc aacgtgcact cggcgttgct gccgtgggag
     3961 tccgtgggga gccgcacgtt caagtggcag ggcaacggct tcgtgaagag tgacgagcaa
     4021 acggagtccc ccaagctcag caaaaaggaa ggcggcaaga agcaccgttc ggatgccaat
     4081 cgaccggcag agccgcctcc cccgcgtccg cccacggcgg acgaaatgtt ggatcgcgtt
     4141 tacgcgctct acaagaaaga gcgcagtgct tccggtcgac cccgcttcga cttcgtgacg
     4201 gacgtcgcgg cggacagcag cccggagcga gtcgtcgttc acggtaaaga catcgtggtg
     4261 ttcggcaaaa acttccgcag cggcgcgagc tacgccttca ttcagatggg agtcgccgac
     4321 gagaaggacg tgattgacgc gaccgcgcgc gatcttaccg gcgacggcaa agcggaaatc
     4381 gtggtgcgag cggtgctgcg tgcgaaggcg agcaaggcgc tgggtgggga caccgtggag
     4441 cgccatgcat tgttggtcta cggcatcaag gaggagcgct tggcccgcgt gtttgccgcg
     4501 gagacgggcc gcgcgctcgg caagaatcag attctcgggg tcgttgcgtt cgagcccgcg
     4561 tcacgcggca cacgcatcga gttgcgcccc gggcgggcca tcggttggac ggagaacagc
     4621 taccccttcc ctcccgacac gacgacggct ggtggcctcg aaccgttgct tctaccgtgg
     4681 tcggggaaca agcgggcgta ccactacaac ggcagcgagt acgtcctcga atgagtcagt
     4741 gagcccgcga atgaacgcac cattcgcggt gcgctcggtg cacagcctgg tttccaatca
     4801 cgtggtgcga agctgcggct cccaccggcc aagtcgatga gcgactcgac tcggccgagg
     4861 gagatggcgc gccgaccgac gcggttcagc gtttgacgcg caacggagct tcttgctcgg
     4921 cgtcgagctc ggccagtgcc cgggccaact cttcctgctc cgcagcctcg agcgctcccg
     4981 gagcgccctc gacccgaacg gctgcctgag gtaccggcgc gggctcgggc ggagcaaggc
     5041 ccatcttcct cttcagggcg agaaggtcgt catccacgcc gtgggctacc tctagctcgc
     5101 tgaatttgtg agccagggcg tccccggtgt actgctcgtg gagctcagcc gaggcttcgg
     5161 cttccgcttc catacgctcg atgttcgagg ccatgcggtc gaacgcctcg aaggcgctgg
     5221 cgttcttgag cccgctcatc gtctcggcga tggccttttg cgcttcggcc cgcttcttcc
     5281 gcgcgatcag caggttcttc ttgcgcttgg cttcctcgat cttgttgttc aaagcgcgca
     5341 acgcgagctt caattgctcg accgcgttct tctgcttttg ccactgctcg aggaactgat
     5401 cgcgcagcgt ctcgtgctct tttttgcggg caagcgcctc tttggcgagg ttctcgtcgc
     5461 cggcacgcag cgcgagcatg gcgcggcgct cccactctgc cgcgcggcca ccctcctccg
     5521 cggcctgctt ctcgagacgc ttctcgtccg cgatgctggc cgcgacttgc tttctcgctt
     5581 cgagcagctg ctcgctcatg tcgacgacga tctggttcag catcttctcg ggatcctccg
     5641 agcggctgat caggtcgttg atgttggatt tgatgagggc tgcgagccga gaaaaaatac
     5701 ccatatctct ctctgccttt tgtctctctg cggcgagcct ttggctctct gctttgaact
     5761 gccgaatatt tagctagcgc tcgcgtcgtg agctcggtgc aagagttcgg acaacgccgg
     5821 aacgtgctcc gcgacggcca gatcgacgtc ggccagaacg ccttcgagtt cggccagatc
     5881 gaggctttcg agcggcaacg ccgccgacag caccacgttc ccgttttgta gcccgtacgc
     5941 tgcatgcagc aacgaagaga cattgagctc gagcagccgt ttgaacagcg gttggctgga
     6001 ctcggcttcc gcgggcgccc gccccatctc cacgcgaacc accaatagcg ggggttccaa
     6061 gcgcaaggcg acgagcggac gattggcccc catagtgaga acgaaggtcc cattctcgag
     6121 ccgctcgaaa cgccgctcga ggcgggtcaa gtacgcggtt aggtcctcac tcgattgaat
     6181 catcggcggg cagcctacca taactcgggt ccgccgatgg cccgagaact ggccccgcgg
     6241 tgctagcccg cgtgccgcag aggatccccg tcccgactgc ggcgcaaacg gaccacctcg
     6301 tccggacgcg gtgattccgg ctgaatcgtg acgtgctcga gcccatgcac gctcttgagc
     6361 cgagccgcca ccatccggca aacctccgtg ccatggctac cccggctcaa caccacatgc
     6421 gcggtgagag cgtcgaactg atcggagatg cgccagacat gtaggtcatg cacttcgctc
     6481 acgccggggc actgcaagat ggtcttctcg atcgccgcaa cgtcgaggtg cggcggcgcc
     6541 gactccagca agatgccgac cgtctccttg atcacccgcc aaccgctgac gaacacgagc
     6601 aaggcgatgc cggcggagag cagaggatcc gcgcgataga cgccccacag ccagacgcac
     6661 agcccgctga tgatcgcggc gacggagccg agcgcatccg tgagcacgtg ggccagcgct
     6721 gcccgtacgt tcagattgtg cttgtggccg cgcattagga tcgcggccac ggcaatgttt
     6781 atcgcgagcc ccgccgttgc ggtaaccgcc accgaccgcc cgtcgatcga gtgcggcacc
     6841 agccagcgct ccaccgactc tttcaggatc agcacggcca ccccagatag ggccataccg
     6901 ttgacgaagg ccgcgagcac ttcggcgcgg cggaagccga acgtgctgcg ctccgtgcgt
     6961 ggacgattcg cgaaccgctg cgcagagagc gctagcccca gcgccgccgc atcggcgagc
     7021 atgtgaccgg cgtctgcgat cagggccaag ctgccgctca cgaagcctac gatggcctcg
     7081 acgaacatga agccggccgt gagcacgagc gctaggaaca gcgtacgcgc cgcttggcca
     7141 tgggcgtgcg aatggtggtg gtcgtgagcg tgctcgtgct cgtgagcatg ctcgtgctcg
     7201 tgctcgtgag catgctcgtg agcatgctcg tgctcgtgct cgtgagcatg ctcgttttcg
     7261 gggtcgtgct catccgcgtg ccggtgcgct acgccttgag caccccgcga ttcagcgcgc
     7321 cgatgcgcac catgctggtg atctcgttgg tcatgagacg tgtggtcgtg aggagcgtgg
     7381 tcgtgcgcac ctcgagaccc gtgctcgtga gcatgctcat gcgagtggcc atgatcgtgc
     7441 tcgtgagcat ggctgtgccc gagctctgaa ttggggtcgt gctgagtcgg ggggcgggat
     7501 gagcgagtca cgtgcgcctc agtctagcct ctcctcacca gatggcgcac ggttccgagc
     7561 accgagcagc gaagcatcga gccgcgagac atcgagcgcc gaagcacaga cttccgctcg
     7621 ttcggattcg gaaacaggaa gccgccgaac cagctcccag ctcccagaaa agatcgcctc
     7681 actgcgtgtc gcgcagctca gcccctcgcg cttggaagcg catggcccgc caaacgacac
     7741 ctcgggcccc ggaggtccgc cgccgccgcg cgccacgaac aaggacaatt catcaataaa
     7801 accagccaca tacaggagaa tcagcaaacc ctgttgcgca ctaccccgcc gtgagtaccc
     7861 ttcgccatgt ttgaccgcaa tgcccgcgaa tgcttgacgt tcgatgacgt catgctcgtg
     7921 cccgcgttca gtgacgtcgt gccctccgag gtggatgttc ggagccgatt cacccgcggg
     7981 attgaattga acgttccgat cgtcagcgct gcgatggact ccgtcactga gtcgcgcacc
     8041 gcgatcgcca tggcgcgcca aggcggcatc ggcatcatcc acaaaaatct gaccatccgc
     8101 gatcaggcgc aagaagtgga tcgcgtcaag cgcgcggaga gcggcattat tttggacccg
     8161 gtcacggtcc ggccctcaca gtccttgcga gaagctttgg ccgtgatgcg tgcgcacgac
     8221 gtgtccggcg tgcccgtggt cgagggcgag cacccggtcg gcatcctgac cgcgcgagat
     8281 attcgattcg aaaagaacct cgatcaaccc gtcagcgcgc tgatgaccac ggagttggtc
     8341 accgttccgc ccggcgttgc cactgagcag gcccgcgagc tgctgcatca acaccggatc
     8401 gagaagttgc tggtggtgga caacggcaag ctcgtaggtc tgattacgat caaggatttg
     8461 ctgcaagccg aacgcaaccc gctcgccgtg aaggacgatc ggggacgctt gcgtgttggc
     8521 gccgcgatcg ggcccggctc ggaccgtttg cagcgcaccg aggcgctcgt cgaggtcggg
     8581 gtggatgtga tcgtgatcga taccgctcac ggtcactcga gccgggtcat cgaagccgcg
     8641 cgggatacca agaagcgcta ccccaacgta cagctcgtag cgggcaacat cgcgacggca
     8701 gaggccgcgc tcgcgttaat cgacgccgga gtggacgcgg tgaaggtggg catcggacca
     8761 ggaagcattt gtacgactcg aatcatcgcc ggcatcggtg tgccacaggt cacggccatc
     8821 gcagactgcg tggaagtggg cgatcgccac gacgtaccgg tgatcgccga tggcggcatc
     8881 aagtactcgg gtgatgtgac gaaagccatc gccgcgggcg cctccagcgt catgatcggt
     8941 tcgctgttcg caggtaccga cgaagccccc ggtgaactcg tgttgtatca aggccgcagc
     9001 tacaaggtgt accgcggcat gggttcgatc ggcgcgatga agcgaggcag caaggatcgc
     9061 tacggtcaag ccggtaccgc ggacgagaag ctcgtgccgg aaggcatcga ggggcgcgtc
     9121 ccgcatcgcg gatctctggc gtcgatcatc gcgcagctgg tcggcgggct ccgctccggt
     9181 atgggctaca ccggctcgct cacggtccag gatctacgaa agaacgctca gttcatccga
     9241 atcacgggtt ccggcttgcg cgagagccac gtgcacgacg tgatcatcac ggaagaagca
     9301 ccgaactatc acgtctgagc gtaccgcgtg cgctcggggc gttcccgagg acgcatgtct
     9361 ttcgagcggc agcggcgctg atggaagtat cgaatcggca agggtcgaat cggcaggttc
     9421 gacccttgcg agcctggttc gcacccgagg acgtacggcg agaccgaagc gatatccgcg
     9481 tcagattggc agggccgttc gttatttgaa gccgttcaag tgcaccacgt accactctcc
     9541 ccgccacgaa atcagggacg tgagcactag ggcacgttcc gcgccttggg cggtctttac
     9601 gaacatgcgt gagcgcagca cgcgaaagta cgggaggcgg ttgccctcct gccccggctt
     9661 catccaacga gcctgatttt cgggaatttc gatgcgctcg aagcggatcc ccgtcgcatc
     9721 cttgccgagc gtcttgtgga gatcgtggat gtcgcgatcg aaagcggtga gtaggcgaaa
     9781 gcgatagtcc cgctccggtt tctcgatggc tttgacctgc tgatacgcaa ccagcggaaa
     9841 gaagctgccg tgggcgaggc tcgggtcgtc gagttctatg gcccgcacca aagccgcgag
     9901 ccgcgccacg aagctcgcgc tgtcggcgcg cggccgctcg tcggtttgag gaagtgggct
     9961 gccgtccgcg cctagcaacg cgggttcaga ggtggggcca ccggcgttag gtgagcccga
    10021 cggagccgac gcgacgcgag cgctctcggc aacacttgga gccctcgcag gcgagccgga
    10081 cgggccactc gagttcgatg ccgcgttggg cattgcagtg ggtgccgcgc tcggcgcggc
    10141 aggcgccgcg cgatgtccgg acggggcgtc cgttacccca agaggttggc ctttcatgcc
    10201 gacgatgccg agcgggccgt tgcgagctgc gctcggcgga gccgcgccgg cttcggcatc
    10261 gtgtcgggat ccggtgcagc ttccggtcga gatcgaggtg cccagcagcg ccccgctcag
    10321 aattgccgcc gaaacggcgc gcctcacgtg caggggcttc ttgcgcaccg cgcgcgctat
    10381 ctcgaatcca gctctcggga tcggcatgat tgacacgtcc gcataccatg aaggctggtt
    10441 ggtcccccaa taagtgggcg tctgggcgcg gtccgcaaac cggaagttcg cgctgctagg
    10501 cggggcagga ccgccaaatc ccccgccccg agctgggtgg ggcttcagaa tcggcgcgaa
    10561 cgcactcatc gcccaatcgc ctcgtcttgg cacctgccat cagcgcgagt taccttcgtt
    10621 cgatggctcg cgagaagatg gcgcaggccg tgtcacgacg tgccttgctt cgtacggcgc
    10681 tgagcgccag cgcgctgctc tcgagtgcgc tgggccgcgt cgcacctgca aacgccgcaa
    10741 aatacagcgc ggctgacggt gagctggaac aacgcctgtt ggagctgccc caagcacaag
    10801 gctcgccgca taaagccagg ctcttgttac ccaaacggat tacgggagaa ttgccgctct
    10861 tgatcctctg ccacggcttg ggtgaaagta atgacgtggc gctcggtcta cgagcgtgga
    10921 ccgagctcta cgggctcttg accgcagtgg agcgcctcgc ccatccgccg gtcgagcgcc
    10981 tatttccgaa gttgaactat ctgcccgagc aaagacttct ggagttgaat cgagccctcg
    11041 ccgagcaacc attgagtcct tgcgcgatgg tctgtccgtt cacacccaat ccgtacgtga
    11101 ggggcggaga ctcgatggat cggatcgagc gattcgctga ctatgtcgag tcgagtttga
    11161 tccccgccgt tcgcaaggag gcgcccattg ccaaaaccgc agctggcgtt ggactggcag
    11221 gggtatccat gggcggctat gtcgcgctcg aactggccgt aagaaggccc acttcgttcg
    11281 gtcgcgtctc cagcgtacag ggcgcgttcg gcgcgcctgc gctcggacgc tataccgaag
    11341 gattattgaa cgcccagcgc acgggagctc tgagagattt gcttctgctc acctcgaggg
    11401 acgacccgtt ccgcgcggca cacgaacgat tggctcgagc actcagcaat tcgacgctca
    11461 gacacgaact tatcgtgaga cccggacccc acaaccaacc gtggttgcgc gaagttgggt
    11521 gtctcgaatt gctgtgctgg aacgatcgcg ctctgcgatc ggccgcgccg tcaccgactt
    11581 agagctgctc cgagcctgta cttcgtgccg acttcggact cgaagggccg aagttcaggc
    11641 tccaactcaa gccgctcggg gcttgaccag gagctcgacg atcgtttgcg ccatgcgttc
    11701 gagaggcacg acgtgctgcg cggccccgag ctggaccgcc tcgcgcggca ttccgtaaac
    11761 cacgctcgaa gcctcgtcct gagcgatcgt ccgtgcgccc gccgtgcgca tggccagcaa
    11821 accctcggct ccgtccgcgc ccatacccgt gagcaccacc ccaaccgcgg cgcgggcggc
    11881 ggcgcgggcg accgaagtta acagcacgtc ccccgccgga cgttgaaagc atacccgagg
    11941 gccgttccgc aatacggtgc gaaaacctcc gtgcgctacc ggctccacga tcatgtggat
    12001 atctggtggc gcgacccgca ccatgcccgg tagcaagacc tcgcccgcgc tgccttcgcg
    12061 gacatcgagc tccgtatctt tcttcaggcg agccgcaaag gaggcgcaaa atgcggcttg
    12121 gatgtgctgc acgatgacca gcggtggagt gttcttcggc aactgagaga gcacccgcgt
    12181 cagcgcttgc gtgccccccg tactggaagc aatcgcgacg atttgatgcc cctgaaagtg
    12241 ctggggctgc gcgcgagccg cgttggtctt ggcgccggat tgccgttgcc gaaccggtgc
    12301 cttggccgcg cgccggatcg cctccacgat ctccttcttt acggcgtcca cgctgaacga
    12361 gccgtgcggc ttcggtatga tctcgaccgc ccccgcgtcc atcgccgcga gcgcgttttg
    12421 actgccgcgc tccgtgacgg cgctgaccat caccacgggc atgggttggt gcttcatcag
    12481 cgcacgcagg aaagacaggc cgtcgagctt gggcatctcc acgtccaagg tcatgacgtc
    12541 ggggtggctg cgcaacagca cctcacgcgc ctctacggga tcggcagcca tacctactac
    12601 ctgcatgtcg ggctgggcat tgagcgtgtg ctccagcatc tttcgcacga cgaccgagtc
    12661 gtccacgatc atcacgcgaa tcttctggct cacgatggcc tactcatttc gatatgcgcg
    12721 gagaggtggc ccgcttcgcc ctcgagccaa agcgcgtccc gcgtctcgct ctccgacgag
    12781 cgttgcgcgc ggggagcagc gagctgaacc agcgcattcg gcgcgactcg actcacgcag
    12841 ccgccgcaca ccatgttcgc gatttcaccc accgccgcga gcgcgtcgtc atccgttaga
    12901 tcgccgggca tctgaccgag cagcgcggcc gcgagcgatc gggcctcgcc cgactcgatc
    12961 gagagagaga attcaccttg caccccgccg ctgaactcga gatcggcccg cagccaatcg
    13021 gtcgaggcgt ggggatcgca cgcggcgggc gactcgtaag cgagcgtttc gagcacctcg
    13081 atcaaagatt cgctgagcgc ttcgtgtagc ttggcttcca ttcaccgcac tcccagcgcc
    13141 gaagacaacg tgcgcaccac gtcttccggc cgaaatggct tcttgacgaa gcctttgacg
    13201 cccaatgctt cgagctcctg gcgccgggct tccgtgccat cggtgctcac gaccactact
    13261 ggtacacgcc ggaggccggc gtgggcgcgc atgcgacgca acagctcggg gccggtcatc
    13321 accggcatgt tcagatccgt aaccaccaga tccacccagc tgcggcgcag cacatcgagc
    13381 gcgctcgccc cgttctcggc ttccacgatt ttttccacga ccagattcga gctctcgatg
    13441 gcgcgcgcta gcaagcggcg catcgtcggt gagtcgtcga cgagcagcag gttgacgcca
    13501 cgcgctcgag ccgtcttctc aacttcccat gttttcacga gcaccttccc cgttccaacc
    13561 tcgagccgta aggtgcggct cgaagtgccg ccaacgtctt cgtgttcaac caggataccg
    13621 cacttccaaa gggcacgccg agccgccaag tggttgcgat gcccgatgtc gaaagccgct
    13681 gtgccctgga acatgttcgc gccgccatac aagcgcacgg ccaagcgccg aggattggcg
    13741 cccagcttcg cgagctcgtt gacgagcacg tgaatccccg tatccccgta cagcgcgggc
    13801 atcttttgcg ccttcgagag gtcgagcgaa gagtccggca gttgaaagtg cagcatgccg
    13861 gccgcgcgcg attgcgcatc ccaggccgtc acaccaatgc acgatcccaa gctgtgcgtc
    13921 acgatcgtcg atgtgggctc gcgactgacg gccagttcac cgatcccgac ggtgtgttca
    13981 atcatacgag tcgcgacgac ggacatcagc cttccccggg taggtggtag atactcgggc
    14041 tgaccagctc gagcggggtc acgacctcgg agagcgtctc cgagctcccg atgaacagcc
    14101 aggcccccgg cgcgagacgc tcgacgacgc gatcgagcag ccgctgccgc gtctccgcgt
    14161 cgaagtagat cattacattg cgcaagaaca cgacctcgaa gcgagtggtg ggcagagggt
    14221 ccgagagcaa gttcaagtac tcgaacctca ccatcgacgt gatgttgcgg ttcacacgcg
    14281 cataaccatc gaactgatcg acaccgcgct cgaaccagcg ccgcacccgt tcccgcggca
    14341 tacgctcgat ctcttgcatc ggatagattg cgcgtttggc acgttcgaga gctcgccgcg
    14401 ag
//
LOCUS       DASTKU010001080         7379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1776346, whole genome shotgun sequence.
ACCESSION   DASTKU010001080 DASTKU010000000
VERSION     DASTKU010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776346"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..1554
                     /locus_tag="VFQ61_38580"
     CDS             313..1554
                     /locus_tag="VFQ61_38580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960459.1"
                     /translation="MAHSAFESCRLSAVVAAAIGVLCGCASRDLVEPAPQEELGSQVS
                     GVHMAELSKDFGIVYLTNTAKDEVVAVDARSLSGDASDDALLNRAYIVSRDSGELDVL
                     DLRTLELSKIKNYGVLNRVSLGAHPTHLSLSRDGRLLAVMEEEEGSGAVSFVDARDMR
                     ELKRLPGFFTPHFMRFAPDGRYGYVANLNAHHLTRVDLEKLEIDAQIPLDGFEAPPHE
                     VLAPDEGGFADAQIDAEGVLYAAHSATNRVLVYDTVKQRKLPELPVGNRPWIVYAEHP
                     FAEVRTHVVPNFGDRTVSLINGAAGDVTATIAAADRESFGVNYSSLVANQAFVMNRFR
                     KNITVVDTVRGEELDTIPVGGNTETAATTRDGRWIVATVSSANRVVVIDAVSHRVVKT
                     FDDVGDYPWAVTIPKGQNYCH"
     gene            1732..2877
                     /locus_tag="VFQ61_38585"
     CDS             1732..2877
                     /locus_tag="VFQ61_38585"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9960460.1"
                     /translation="MTLVLAAADSKDALRRELTQTYGKPAREVRAGVFDCGFELSAIA
                     PAPYLVFARQILPEPHDVSASSIRAWAGVLVEAIAGVLPDSEPWSLHVYPVKEVLEQT
                     RVGARAWHSQTRGGRHGDASREALDARPGARAESAVGSHRCRLIQEAVLELLQKRRRH
                     LLRSLRRDSGLFLENEALVQLALTAPDAGFLSIAAAPLPHTQRRVLSNFAGGELPLAV
                     DKQAPSRAFAKLVEAELRMGRRIAAHDSCVDLGAAPGSWTYVALKRGARVTSVDRAEL
                     RADLMAHRNVEFQQGDAFKFEPAAPADWLLCDVIASAERSAELLVKWLRERWCLHFVV
                     TLKVDDAGSSPVLARLKQELPALTSELWLQRLCANKKEVCAFGTIAD"
     gene            2883..3566
                     /locus_tag="VFQ61_38590"
     CDS             2883..3566
                     /locus_tag="VFQ61_38590"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET9960461.1"
                     /translation="MAMNVGFLLFPRFTQLDLTGPYEVLSRLPSVSMHLVSETLDGAR
                     SDTGLTLAPTHTFESAPQLDLLCVPGGAGVNAVIESDAALTFLEEQGARARYVTSVCT
                     GALALGAAGLLRGYRATTHWRYLELLSRFGAEPVKQRVVIDRNRVTGAGVTAGIDFAL
                     TLAAELYGEKLACEIQLYIEYDPAPPFASGSPQIAAEQLVYSVRDKTRDAYAERVAVL
                     DRWEARRRG"
     gene            3764..4510
                     /locus_tag="VFQ61_38595"
     CDS             3764..4510
                     /locus_tag="VFQ61_38595"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HET9960462.1"
                     /translation="MAGGIDPTRYQTLYDAFSAALRGSVRIPLFSLPVRSIIFRSVAA
                     DYKGMAPTKDTARTIYGGATTTTNRWTGQRGSLRPGIGGLYVSVHLDAMSNEVFHYGK
                     GAGSSIDALTKTRTFRFETERPLFFTDLALGAGRANPFLSELGETSSVKAALRATGLN
                     SVYQGYSHPTDYSMCRALGHALLDANPLTDGLRVTTARDEYAKIVGETSENLVIYDKD
                     SIPLPYLKPLTETVYGKAPDGRLTQTVKRL"
     gene            4878..5804
                     /locus_tag="VFQ61_38600"
     CDS             4878..5804
                     /locus_tag="VFQ61_38600"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET9960463.1"
                     /translation="MALLKDLSAESRQCLAVEHLIGRSSSCMTRLDARCVSSTHACIR
                     WNGARWLLQDLGSRNGTYLNDVRIVATEWQVLSVGDRLAFGDRNRPFVLLDASAPQAM
                     VLALESGHVLPVRDGMIAIPGEDEPLATIYWSSNAEWVLDRAGELIPLKDSQYFDVGD
                     CGYRFSCPQSIHETSVITDGPAVVDLFSAQLSFCTTKDEESVEVAVRHARGVLNLGSR
                     AAFYLLLVLARQREQDRARGVAVHEAGWTSLEHLKQMLKYDEAQLNMDVFRIRQRFQQ
                     AGIIHGAQIIERESRNKCLRIGAARSEFVTLS"
     gene            5893..6699
                     /locus_tag="VFQ61_38605"
     CDS             5893..6699
                     /locus_tag="VFQ61_38605"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET9960464.1"
                     /translation="MTSESVKDRGSLVIVGTGIQWSSQMSAAARKCISSADRVLYALV
                     DETAANFIRGLNPNAASLPYPLDRSLRRDTYRSMADAICAPVRAGERVCAVFYGHPGV
                     LATAAHLALRELRRDGYPARMLPAISAFDCLLADLSIDPGQAGCQIYEATDFLKHARI
                     HDPRVPLVLMQVGAICNPGFFNPLQEEVSSTGLDVLVTRLEQQYPARLEVVLYEASAD
                     PAEESAILRLPLRELRNADVSDLSTLYVPPIETAIAGVTAAGRASISSAL"
     gene            6760..>7379
                     /locus_tag="VFQ61_38610"
     CDS             6760..>7379
                     /locus_tag="VFQ61_38610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960465.1"
                     /translation="MMTSDGEARARELATEAERLRCLGLNIAAIAKFNDALGELGNST
                     ARLKSWCLAHRGSARSELGDTEGAIADLDAAVELRKGEYAWALAHKGEAYRIFARGLA
                     THTEEDHRRFSEAIENACQAFQAACVLDGHSAWTHAHLGATLTLKLWHESSWTKAELK
                     AQKQSFDAAKAALDHALELQPTYPWALQFEALLFGIGGDELAGKDW"
BASE COUNT         1409 a         2264 c         2305 g         1401 t
ORIGIN      
        1 gggacggcgc gttttctgcg aggaaacaga gaatcgtcga catcattcta gaatcgccga
       61 gcctcgctcg tcggccgaag gccgcacctg tagtgcaccc gaaaccgacc ggtaagaagg
      121 ttaccggtgg cttctggact ggttgatcga gcctttcgat agctggattc aaaaccgttc
      181 accacccttc atggtcggcc tgaacgcatt gaatctgcat tccgattcgg tgtcgtccca
      241 ctcagcaggt tctattccag ggcccggctc ggtacggacg tttatcgatg gtcgtcgagg
      301 aggagagaca cattggctca ttcagcgttc gaatcgtgca gattgtcggc ggtggtcgca
      361 gccgccatcg gcgtactttg cggctgtgct tcgcgagact tggtagaacc cgcgccccag
      421 gaagagctcg gctctcaggt ctcgggcgtc catatggccg aattgagcaa agacttcggc
      481 atcgtctacc tgacgaacac ggccaaagac gaagtcgtag ccgtggatgc ccggagcttg
      541 agcggtgatg catccgatga cgcgctgctc aaccgcgctt atatcgtgag tcgcgactcg
      601 ggcgagctcg atgtcctaga cctgcgaacg ctggaactat ccaagatcaa gaattacggc
      661 gtactgaacc gcgtctcgct cggcgcccac cccacgcatt tgtcgttgag ccgcgacggt
      721 aggctcctgg ccgtcatgga agaggaggag ggcagcggcg ccgtgtcttt cgtagacgcc
      781 cgggacatgc gggagctcaa gcgtctgccc ggcttcttca ctccccactt catgcgcttc
      841 gctccggatg ggcgctacgg ctacgtcgcc aacctcaatg ctcaccacct gacgcgggtg
      901 gatctagaaa agctcgagat tgacgcgcag attccgctcg acggtttcga agcaccgcct
      961 cacgaagtgc tggcccctga cgagggtggc ttcgcagacg cccagatcga cgccgaaggc
     1021 gtgctctacg cggcccactc cgcgaccaac cgggtgctcg tctacgacac ggtcaaacag
     1081 agaaagctgc cggagctgcc ggttggaaac cgaccctgga tcgtttacgc agagcacccg
     1141 ttcgccgagg tgcgaaccca cgtcgtaccc aacttcggag accgcaccgt ttcgctgatc
     1201 aatggcgcag ccggcgatgt cacggctacc atcgcggccg cggatcgcga atctttcggc
     1261 gtcaactact cctcgctcgt ggcgaaccaa gccttcgtga tgaatcgctt tcgcaagaac
     1321 atcaccgtgg tcgatacggt tcgaggcgaa gagctcgaca ccatccccgt cggaggtaac
     1381 acggagacgg ccgccaccac acgtgacggt cggtggatcg tggcgacggt ttcgagcgcc
     1441 aaccgagtgg tcgtaatcga cgccgtcagc caccgcgtcg tcaagacgtt cgacgatgtc
     1501 ggcgactatc cctgggcagt gacgatcccc aagggccaga actactgcca ttgatcagga
     1561 gcgaggcgcg tgggacgcgg cggtcctcgg cgcggcccac acgccatttc gcccccgcgc
     1621 agtccggggc aggctcgaga cgctcggctc ttaccgtctc tcctcgattg gttcttaggt
     1681 tcgctcgggg ctgcttggcg cggcgcgccc catgctgtag acagcgcgac tgtgaccctg
     1741 gtgctcgcgg ctgccgactc taaagacgcg cttcggcgcg aactcacgca gacctacggc
     1801 aagccggcgc gcgaagttcg agccggcgtt ttcgactgcg gcttcgagct atcagctatc
     1861 gctcccgcac cctatctggt cttcgcacgg cagatcttgc cggagccgca cgacgtgtca
     1921 gcaagctcga ttcgggcatg ggcgggcgtg ctcgtcgagg cgatcgcagg cgtgttaccc
     1981 gattcggaac cgtggtcact gcacgtctat ccggtcaaag aggtcctcga acagacgcgc
     2041 gtaggcgccc gggcttggca ttctcagacc cgaggcggtc gtcatgggga tgcgtcgcgt
     2101 gaggctctcg acgcgcggcc aggcgcacga gcagaatcgg cagtcgggag ccatcgttgt
     2161 cgtttgattc aggaagcagt gctcgagctg ttacaaaagc gtcgacgcca cttgttgcgc
     2221 tctttgcgcc gcgattccgg cctatttctg gagaacgaag cgctcgttca gttggcgctc
     2281 accgcaccgg acgcgggctt cctgtccatc gccgcggctc cgctacccca cacccagcgc
     2341 cgcgtgctct cgaatttcgc ggggggcgaa ctgcccctcg cggtagacaa acaagccccg
     2401 agtagggcct tcgccaaatt ggtggaggcg gagttgcgca tgggacgtcg catcgcggct
     2461 cacgattctt gcgtcgatct cggcgcggcc ccgggcagct ggacgtacgt cgcattgaag
     2521 cgaggcgctc gcgtcaccag cgtggatcga gcagagctgc gagccgatct gatggctcac
     2581 cgcaacgtcg aatttcagca gggcgatgcg ttcaagttcg agccagcggc accggcggat
     2641 tggctgctct gtgacgtgat tgcaagcgcg gagcgctcag cggagctgct cgtgaagtgg
     2701 ctgcgcgagc gctggtgttt gcacttcgtc gtaaccctga aggtagatga tgcgggcagc
     2761 agtccggtgc tcgcgcggct caagcaggag ttgcccgctc tcacgagcga gctgtggttg
     2821 caacgcctct gcgctaacaa gaaggaggtc tgcgctttcg gcacgatcgc agactgaacc
     2881 gaatggcaat gaacgtaggt tttctactct ttcctcgttt cacgcagctc gatctcacgg
     2941 gaccctatga ggtgttgtcg cgacttccga gcgtatcgat gcatctcgtc agcgagacac
     3001 tcgatggcgc ccgcagcgat accggtttga cattggcgcc gacccatacg ttcgagagtg
     3061 cgccccaact cgatctgctc tgcgtcccgg gcggcgcggg tgtcaacgcc gtgatcgaga
     3121 gcgacgcggc gctcacattc ttggaggagc aaggagcacg ggcacgctac gtgacctcgg
     3181 tctgtacggg cgcgctcgca ctcggcgccg cgggtctact tcgtggatac cgagctacta
     3241 cccattggcg ctacctcgag ctcctgtctc gcttcggtgc tgaacccgtg aagcagcggg
     3301 tcgtgatcga tcgcaaccgc gttacggggg cgggcgttac ggcaggaatc gacttcgcgc
     3361 tcaccctcgc ggccgagctc tacggcgaga agctagcgtg cgaaatacag ctctatatcg
     3421 agtatgatcc ggcgccgcca ttcgccagcg gctcaccgca gatcgcggct gagcagttgg
     3481 tttattccgt gcgagacaaa actcgcgatg cttatgcgga gcgtgtcgcg gtgttggacc
     3541 gctgggaagc acgtcggcgc ggttgacgtg cttcgaacag ctgccgaacg gctggtagca
     3601 ggccgtggcc cgagaaccgc cgcccgaatg caagcaaggc aagagccgct caaatcgaga
     3661 aacagctgag cgaagttcga aggcccgtgc gcaaaaatcg taagtggggg tgcgcgagca
     3721 ccgcgtcgat tgcattcggc gaacaatttc gtatggttcg ggcatggctg gtgggattga
     3781 tccgacgcga tatcaaacct tgtacgatgc gttttcagcg gcgctgcgcg gaagtgtgcg
     3841 gattcctctg ttctcgttgc ccgttcgatc catcattttt cgtagcgttg cggcggacta
     3901 caagggcatg gcgcccacca aggatacggc tcggaccatc tacggtggcg ccacgacgac
     3961 caccaatcgc tggaccgggc agcggggtag cttgcggccg ggcatcgggg gcctgtacgt
     4021 gagtgtgcac ctcgacgcga tgtcgaacga ggtctttcat tacgggaaag gcgcaggctc
     4081 ttcgattgac gcgctgacga agacgcggac ctttcgcttc gaaacggagc gaccgctctt
     4141 cttcaccgat ctcgctctgg gggcgggccg tgccaatcca ttcctcagcg agttgggcga
     4201 gacctccagc gtcaaagccg cccttcgcgc caccgggcta aatagtgtgt accaggggta
     4261 ctcgcatccg acggactact cgatgtgccg cgcgctcggg cacgcattgt tggacgcaaa
     4321 tccgctgaca gatgggctgc gcgtcacgac cgcgcgcgac gaatacgcca agatcgtcgg
     4381 cgagacctcc gagaacctcg ttatatatga caaggattcg atcccgttac cgtatctgaa
     4441 gccattgacc gagaccgtct acggcaaggc tcctgatggc aggcttactc aaaccgtaaa
     4501 gcggctgtaa cgccgcccca cgggtgcgcg gcgcggccga aaacagcgcc gacagcgagc
     4561 ccatggcagc gccagaacgc aaaccgcgca gtgtaagatc cggaacggtg cacgcgcctg
     4621 agtgaaaatt agacgtgctc ggagctcgag cggctaccca gcgcggtaaa ttgccggcga
     4681 agcgaaggag cagcgcggca ggagcagcgc agcaggagcg gcacaggctg gtccgtgcga
     4741 gccatccgca acctaactcc aagctggcca ggcggccgca gtgggccgca atactctacg
     4801 ctcgtctttc gaactagggt gcgcttacat cacggttggg agtgggtcgg aatcagcttc
     4861 ccacccaggc atgcgtcatg gctctcctga aagatctcag cgctgaaagt cgccaatgtt
     4921 tggcggtcga gcacctgatc gggcgctcgt cgagttgcat gactcgcctg gacgcacgtt
     4981 gcgtatccag cactcacgcc tgcattcgct ggaacggcgc gcgctggctg ctgcaggacc
     5041 tcggaagtcg taacgggact tacctaaacg atgtacggat cgtcgcgacc gagtggcaag
     5101 tgctgagcgt tggcgatcgg cttgctttcg gagaccgcaa tcgcccattc gtgctgctgg
     5161 acgccagcgc tccccaagcc atggtcctgg cgttggagtc gggccacgtg ctcccagttc
     5221 gtgatggcat gatcgccatt ccgggagagg acgagccact cgccaccatc tattggtcgt
     5281 ccaatgctga gtgggtgttg gaccgcgcgg gagagctcat tccgctcaag gactcgcagt
     5341 acttcgatgt aggtgattgc gggtaccgat tttcttgtcc gcaatcgatc cacgaaacga
     5401 gcgtgattac ggacgggccg gccgtagtcg atctattctc ggctcagctc tcgttttgta
     5461 cgacgaaaga cgaagaatca gtcgaagttg cggtgcgcca cgcgcgtggg gtactgaacc
     5521 ttggctcccg agccgccttc tatttgctgc tggttttggc tcggcaacgc gagcaagacc
     5581 gggcccgcgg ggtcgccgtg cacgaagccg gctggacgag cctcgaacat ctgaaacaga
     5641 tgttgaaata cgacgaggcc cagctgaata tggacgtctt tcgtattcga cagcgctttc
     5701 agcaggccgg catcattcat ggcgcgcaga tcatcgagcg cgagtctcgc aataagtgtc
     5761 tgcggatcgg cgcagcacgc agtgagttcg taacattgag ttgaacttct ccgcgctgat
     5821 gcggctcgat cgggcggcta ggcccgccga attcccaagt atcatcgaga cacctatgac
     5881 atcagggcac ctatgacgtc agaatctgtg aaagaccggg ggtctctggt catcgtcggc
     5941 actgggattc agtggtcgtc acagatgagc gccgccgcgc gcaagtgcat ctcgagcgcc
     6001 gaccgggtgc tctacgcctt ggtcgacgag acggccgcga atttcatccg cgggctcaac
     6061 cccaacgcag cttcgctccc ctaccctctg gatcgcagtt tgcgtcggga tacgtaccgg
     6121 agcatggccg acgccatctg cgctcccgtt cgcgcgggag agcgggtttg tgccgtattt
     6181 tatgggcacc cgggcgtgtt ggcgaccgcc gcccacctcg cgctccgaga gctacgccgc
     6241 gacggttatc cggcacgcat gctccccgcg atatccgctt tcgattgtct tctcgcagac
     6301 ctctcgatcg acccgggcca agccggctgc caaatctacg aagccaccga tttcctcaag
     6361 cacgcacgca ttcacgaccc gcgcgtacca ctcgtactga tgcaggttgg agccatctgc
     6421 aatcctggct ttttcaatcc tctgcaggaa gaagtaagta gtaccggact cgacgtgctc
     6481 gtgacacgac tggaacagca gtacccagca cgactcgagg tcgttctgta cgaagcctcc
     6541 gccgatccgg cggaagaatc cgccattcta cggctgcctc tgcgcgagct gcggaacgcc
     6601 gatgtttcgg atctatcgac actctacgtg ccgccgatcg aaaccgcaat tgcgggtgtg
     6661 acggctgcgg ggcgggcgtc gatatcgagt gcactgtagg tttgaacccg gatttgcgct
     6721 gcgtgaacgc acgcgagcgc gcaccacagc aaggagacaa tgatgacatc ggatggagaa
     6781 gcgcgagcca gagagctcgc gacggaagcc gagcggctca ggtgcctcgg cttgaacatc
     6841 gccgcgatcg ccaaattcaa tgacgctttg ggcgagctcg ggaactcgac agctcgtctc
     6901 aagagctggt gcctcgcgca ccgcgggagc gcgcggtcgg aactcgggga caccgaagga
     6961 gcgattgcag atctcgacgc agccgtggag ctccgtaaag gcgagtacgc gtgggccctc
     7021 gcccacaagg gcgaggccta ccgcatcttc gcgcgcggcc tcgcgaccca caccgaagag
     7081 gaccatcgtc ggttctcgga agccatagag aacgcctgcc aggcttttca agcagcttgc
     7141 gtgttggacg ggcacagtgc ctggacccac gcccatctgg gcgccactct cacgctgaag
     7201 ttgtggcacg agtcatcgtg gacgaaagcc gagctcaaag cacagaagca gagcttcgat
     7261 gccgcgaagg ccgccctcga tcacgcactg gagcttcagc ccacctaccc ttgggcgctg
     7321 cagttcgaag cactattgtt cgggatcggc ggggacgaat tggccggcaa ggactggta
//
LOCUS       DASTKU010001081         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1776460, whole genome shotgun sequence.
ACCESSION   DASTKU010001081 DASTKU010000000
VERSION     DASTKU010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776460"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2136)
                     /locus_tag="VFQ61_38615"
     CDS             complement(<1..2136)
                     /locus_tag="VFQ61_38615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960466.1"
                     /translation="MQPPAGYASRLLPLLLSLGFALSCAEERAPINRVQPNALEKKFF
                     VGEKLSDPSDNPEFYYRPTIVDVDYGASQEGLFTASYAQTLSRVGFEITEDLLLARLT
                     YERVEGTSGKGAPDDNAGQIVAAFKILRHFDVQSDYNPQTGEELNIVEENDTDRPWYQ
                     RKFMRVDWSQNLITTAYELDTLATLKAYSGDAISYEAAQYYVEDPTSPDAPQFNRNDG
                     YFDVTNKVFAKPQTLNSPWGSIPLCWLSSDFLSGTAPVGNCNPVELKVRLAFKRVVDR
                     DYEPVDWNGQRMSMFGVFTTDDPPRLGYDRNYGVVDAKWRRFAHRHNIWQNSHDRDAQ
                     GQAISCRDDSACRVAAGSHCDGYRAQCTLPYRERQIRTVPFYYGPDSDPTLFESARAA
                     LSEWDQSVRHAVVTARYAECIHEQGGVTSEDARGACNEQYNPSQENALASVPEIFVFC
                     HNPVVAGDPEACGKAGLSARVGDLRYNMANIIQAPQVPSPWGIMVDGTDPISGEVVAA
                     SANLWNAVTDLRTQDVINTMRWYLGELQSNDIIDGNYVKPINAVGSASQGALPDRTSP
                     VSEREIEDRLGAMDDGLAARDARTGARIEAALPAGITAKNLVDWASEETRLQFGDAAL
                     GLGNSPIHSRLAAIQGTPVENALINDSFRMLAGIGADEPLNQTTLALASPLRTGFWQN
                     RSALERERQLKLAARGTCTLEAEAAEGTAI"
     gene            complement(2299..3600)
                     /gene="hisD"
                     /locus_tag="VFQ61_38620"
     CDS             complement(2299..3600)
                     /gene="hisD"
                     /locus_tag="VFQ61_38620"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET9960467.1"
                     /translation="MNQRLLRSVAYGTPAYHETLAALARRGESDLTRVEPAVREILSA
                     VQREGDAAVARFVERFEHRRPGPLLVRDYGGAEALASLSSEVRNALELSAVRVRRYHE
                     HQRDHLGNFAYEEEGVHLAARVTPLDRVGVYAPGGKAFYPSSVLMSAVPASVAGVKEI
                     YVACPQVNAAVRAACHLAQVTGIVDAGGAQAIAALAYGTQSVPRVDKIVGPGNLYVTA
                     AKRLVFGEVDIDGLAGPSEILVIADDSADPAHIAADLLSQAEHDEAAYPLLIASEQSI
                     VDAVELELARQLAELPRKNIAEPSLRNNGMALVIRERAEMLRAANVLAVEHVAIQTHE
                     PREVSRGVLRAGALFVGPMTPESAGDYVAGPSHVLPTGGAARFGAPVGVYDFVSRSSV
                     IEYSPAALQRQAAAITAFARSEGLEAHARAVEIRTKNASQR"
     gene            complement(3597..>3950)
                     /locus_tag="VFQ61_38625"
     CDS             complement(3597..>3950)
                     /locus_tag="VFQ61_38625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960468.1"
                     /translation="DTGELRSGTAPNGWRPSWIVIGHSTLLGDPYFLDVGSPDAEGDC
                     PVFTAMSGTEQWKPRLCASSFAMFLRILAIGMEVAKGFPEDDIDMDDEQVFREALGPK
                     IREYDPAALKAGHWT"
BASE COUNT          678 a         1296 c         1231 g          745 t
ORIGIN      
        1 gatcgcagtg ccctctgcgg cttcggcctc caaagtacaa gtcccgcgcg ccgcgagctt
       61 cagctggcgc tcgcgttcca aggcgctccg gttttgccag aaacccgtgc gcagcggtga
      121 cgcgagggcc agcgtggtct ggttcagcgg ctcgtccgcg ccgatcccgg cgagcatccg
      181 gaaggaatcg ttgatgagcg cgttctcgac cggcgtgcct tggatggccg ccaagcgtga
      241 atgaatcggc gagttcccga gcccgagtgc ggcgtcgcca aactgcagcc gcgtctcctc
      301 gctcgcccag tccaccaggt ttttggccgt aattcccgcg ggtagggccg cctcgatgcg
      361 ggcccccgta cgcgcatctc gcgccgcgag cccgtcatcc atcgcaccca ggcgatcctc
      421 gatctctcgc tcggaaaccg gcgacgttcg gtcgggtagc gcgccttgac tggcggagcc
      481 caccgcgttg atgggcttga cgtagttgcc gtcgatgatg tcgttgctct gcagctcgcc
      541 gaggtaccaa cgcatcgtgt tgatgacgtc ttgggtgcgc agatccgtaa ccgcgttcca
      601 aaggttggcg ctcgccgcga cgacttcccc cgagatcgga tcggtgccgt ccaccatgat
      661 tccccaaggc gatggcacct gcggtgcttg aatgatgttc gccatgttgt aacgcaagtc
      721 acccacgcgc gcgctcagcc ccgctttccc gcacgcctcg ggatcaccag ccacgacggg
      781 gttgtggcag aacacgaaga tctccggcac gctcgcgagc gcgttctctt ggctcggatt
      841 gtattgctcg ttgcaagcgc cacgcgcatc ttcactcgtc acgccaccct gctcgtggat
      901 gcactccgcg tagcgcgccg tgaccaccgc gtgccgcacc gattggtccc actccgaaag
      961 ggcggcgcga gcggactcga acaacgtcgg atcactatcg gggccgtaat agaagggtac
     1021 ggtccgaatt tgacgctccc gataaggcag cgtgcactgc gccctgtagc cgtcgcagtg
     1081 cgacccggct gcgactcggc aggcgctgtc gtcccgacag ctgatcgcct gtccttgcgc
     1141 gtcccggtcg tgagaatttt gccagatgtt gtgacggtgc gcgaagcggc gccacttggc
     1201 gtccactacg ccatagttcc ggtcatagcc caggcggggt gggtcgtcgg tggtgaacac
     1261 accgaacatc gacatgcgct gcccgttcca atccaccggc tcgtagtcgc gatccacgac
     1321 gcgcttgaac gccagtcgca ccttgagctc gaccgggttg caattgccga ccggagcggt
     1381 gccgctcaga aaatcgctgg atagccagca caaggggatg ctgccccaag gcgaattcaa
     1441 cgtctgcggc ttggcgaata ctttgttggt gacgtcgaag tagccgtcgt tgcggttgaa
     1501 ctgtggcgcg tcggggctgg tgggatcctc gacgtagtat tgtgcggcct cgtacgagat
     1561 cgcatccccg gagtaggcct tcagggtggc gagcgtgtcg agctcgtaag ccgtcgtgat
     1621 caggttctgg gaccagtcga cgcgcatgaa tttgcgctgg taccaggggc gatcggtgtc
     1681 gttctcctcg acgatgttga gctcttcgcc agtctgcggg ttgtagtcgc tctgcacatc
     1741 gaagtgacgc aggatcttga acgcggccac gatctgcccc gcattgtcgt ccggtgcgcc
     1801 cttgccgctc gtgccttcga cgcgctcgta cgtcaggcga gcgagcagca gatcttccgt
     1861 gatctcgaag cccacgcgcg acaacgtttg cgcgtagctc gccgtgaata ggccttcttg
     1921 gctggcaccg tagtcgacgt cgacgatcgt tggtcggtag tagaactctg ggttgtcgct
     1981 gggatccgac agcttttcgc cgacgaagaa cttcttttcg agcgcgttcg gttgaacacg
     2041 attgatcgga gcccgttctt ccgcgcacga gagcgcgaac ccgagtgaga gcaacagcgg
     2101 caatagacga gaggcgtacc cagcgggagg ctgcatcgtg ttctccagag ctgaacgata
     2161 acctcgaacc cggctggatg gagcgcgcgc ggagagtttc tggcgagttg accactccag
     2221 cagtcgttgc ggcaaactct cgggcgcgct ctgggtgtcg caagcgcgct cgcgtggagc
     2281 gcattcgaac ggaaagtttc agcgctgact ggcgtttttc gtacgaatct cgacggcccg
     2341 cgcgtgcgcc tcgagccctt cactacgcgc aaatgccgtg atcgcggcgg cttggcgctg
     2401 cagcgcggct ggcgagtact cgatgaccga gctgcgtgac acgaagtcgt aaacaccgac
     2461 aggcgccccg aagcgcgccg cgccacccgt aggcaaaacg tgtgacggtc ccgccacgta
     2521 gtcccccgca gactctggag tcatgggccc gacgaacaga gcgcctgccc gcagcacgcc
     2581 tcgcgaaacc tcgcgcggct cgtgcgtctg gatggctacg tgctccacag ccaaaacatt
     2641 ggcggcgcgt agcatttccg cgcgctcgcg gatcaccaac gccatgccgt tgttgcgcag
     2701 cgacggctct gcaatgtttt tgcggggaag ctccgccaac tgccgcgcga gctccagttc
     2761 gaccgcgtcc acaatcgatt gctcactcgc gatgagcagc ggatatgccg cctcatcatg
     2821 ttccgcttgg gagagcaggt cggccgcaat atgcgcggga tctgcggaat catctgcgat
     2881 cacgaggatc tcactcggac ccgccaggcc gtcgatgtcg acttcaccga acacgagccg
     2941 ttttgcggcg gtaacgtaca ggtttccggg acccacgatc ttatcgactc ggggcacgct
     3001 ctgcgtccca tacgccaatg cggcgatggc ctgcgctccc cccgcgtcca cgattcccgt
     3061 gacctgggcg aggtgacacg cggcccgtac ggcagcgttc acttgcgggc acgcgacgta
     3121 aatctccttc acgcccgcca cgctggctgg caccgcgctc atcagcacgc tcgacggata
     3181 gaacgctttg ccaccaggcg cgtagacgcc cacacgatcg aggggcgtga cgcgcgccgc
     3241 caagtggacg ccttcttcct cgtaggcaaa gttcccgagg tggtcacgct gatgctcgtg
     3301 gtaccgacgc actcgaactg cggaaagctc gagcgcgttc ctcacctcgg aggacaggct
     3361 cgcgagtgct tccgcacctc catagtcgcg cacgaggagc ggccccggcc tgcggtgctc
     3421 aaagcgctcg acgaagcgcg ccacggcagc atcaccttcg cgctgcactg cgctgaggat
     3481 ctcccgaacc gcgggctcta cccgagtcag atcactctcg ccacgccgcg ctagtgcggc
     3541 gagcgtctcg tggtaggccg gagtaccgta tgcgacggaa cgcagtagcc tctggttcat
     3601 gtccagtgcc cagccttcaa agcggccggg tcgtactcgc ggatcttggg tcccagcgct
     3661 tcgcgaaaca cctgctcgtc atccatgtcg atgtcgtctt cggggaagcc cttcgcgact
     3721 tccataccga tcgccaggat gcgcaggaac atggcgaagc tcgaggcaca cagccggggt
     3781 ttccactgct cggtgccgct catagcggta aacaccggac agtcaccctc cgcatcgggg
     3841 ctcccaacgt ccaaaaagta gggatctccg agcagcgtgc tgtgcccgat cacgatccag
     3901 ctcggccgcc agccgttggg agcagtcccg gaacggagtt ccccggtgtc
//
LOCUS       DASTKU010001082         5617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_435752, whole genome shotgun sequence.
ACCESSION   DASTKU010001082 DASTKU010000000
VERSION     DASTKU010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435752"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VFQ61_38630"
     CDS             <1..994
                     /locus_tag="VFQ61_38630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960469.1"
                     /translation="QEQEHMQYVGNSKIILSKSAAGLGRAGVMLGALAALAAGCGRLE
                     EFPDSDVGRAASAATICVTKEPNGPRGLLHTVSSVGSNQTRLAALANRELQLSVTQFW
                     NPNTTTGVYNNHPVRTRFDGSSWIIETTDGGSLALGAAFNVLYSEPKILTLQSPSLIQ
                     SAPGPLGDAQPSIVDTKITNKAVLKNGPSSIGSDKLPPFFTSGKYAELTDSLSPSSKT
                     AGDPPPEECPPDTGEHRAQPGVLVSERRYIDHVASSDNALYNYTCIPADSLLTRDALL
                     IVEPGETTRDEERALGVWYRSPEHGLGSWCIFYQDVNLTMAPGTRFFVQVWTPE"
     gene            1177..1545
                     /locus_tag="VFQ61_38635"
     CDS             1177..1545
                     /locus_tag="VFQ61_38635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960470.1"
                     /translation="MKNLLRSLVITAGLVSFAGAAWAGDYHACKVTDVQHVKVGTTNY
                     FVFKANCSDGTYFWSAPTDAIGTPLMSSLVTTAFTSGRLINVQCGMNGGSCGAFSTKV
                     YDANVHTTQTVWTAETVNLR"
     gene            complement(1671..4442)
                     /locus_tag="VFQ61_38640"
     CDS             complement(1671..4442)
                     /locus_tag="VFQ61_38640"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET9960471.1"
                     /translation="MRLPAFKCKPFIRRRPAANWLGLIALAVLAAVVLFQWRIRDEVT
                     TRPALRWDVTFKDFEGRAIPVLAMGSHPPTHSPERSPIDSILEVRIQGLGPGQFAVLF
                     LFDSTGQSVRPALPAELPFERLADVEVSLPLRSLRPLTGATRALLAYGRKGQLESALR
                     QLATAAPKELSLKETIEKIQDFDLGSSIRSVRIPNLEPQYSSTVLDAAEAALEHGDLM
                     ASLVRVEGASGQYPSSDLARAAGIGAEAAFGLGRIGLARRLSETGLAAKWKGGHWNAR
                     LRRVQILARAEGDVSADPPAERELLRKTEGERSAPIESALDAALDFRLALLHDRFSTA
                     PPRAAAAARRLIEHAEGLPPSESRWRELAAPLCRAATSISKTEPTLGQRWVKRGLELA
                     QPHRAAEAYCMIAAADAARHASNLEESERFLERARAQLADAILPREQREVWFSTALQQ
                     SERGLPAEALIAAKRAAQWVTFLLALEVDVADREALLTNALGYYGAAARLGAVANLGV
                     DAIALAEAGKGRGLFALLRGSTASGARAISPMRDLHLEPARSTGTAPDPESRRGDPFA
                     RELGTVEEMARRLEQSDAVLSYTFLGANSQRRPEIAVGVLTSEGASMTLVEQPETLLD
                     DVVTLAAGVEHNDEFAAREVGKRLHATLIEPVQSQLQGKTRLFISPHLRLHTLPWAAL
                     YDGSSFLIERFGIARMLPLTLAEAGWRDAQTVFEHGNQTNWVLAVNARHPTFPPLPGF
                     DPLIERARSAFTRVTLLGEREATRTALLSSLGAADAALFAGHASYDPARPLRSALYTS
                     AKQADTSAWPEDRVEARDLMQLRNRLSTVVLLGCETARLWRGRSSYSDEAIGLARALV
                     LSGSRTVIGALWPVLDRDAEDFSRALLGMESSLDIIRHVQEVSRCLASQRCPNRGIAT
                     WASFLVDTR"
     gene            4732..>5617
                     /locus_tag="VFQ61_38645"
     CDS             4732..>5617
                     /locus_tag="VFQ61_38645"
                     /inference="COORDINATES: protein motif:HMM:NF013199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960472.1"
                     /translation="MKARLNSPQRGPWVFTSVLLALAAPGLTQCGSQDGDSGANQLDH
                     VEEEAAKQTSPLGTFNVLTRSYNNQRTGANLSETQLTPANVNSRQFGKLFQLPVDDEV
                     YAQILYASGVSIGGATRNVIYVATVNNTVYAFDADTAGAPLWQRNFNGSGRPPRHGEV
                     ANGTACGGNYHDFAGNIGIVGTPVINASSNTMYFVTRTLESGSMIYRLRAVNIATGAD
                     RAGSPKQITASVAGTGDGSNGTTIAFNPMFHNQRAGLALASNAVYVAFAAYCDAPPTH
                     GWVMGFDATSLASTGVFNT"
BASE COUNT         1116 a         1747 c         1743 g         1011 t
ORIGIN      
        1 gcaggagcag gaacatatgc agtatgtggg gaactcgaag atcatcctaa gtaagagcgc
       61 ggccggtttg gggcgggctg gagtcatgtt gggggcgttg gccgctttgg cggcaggatg
      121 tgggcgcctc gaagagttcc cggactccga tgtcggccgc gccgcgagcg cagcaacaat
      181 ctgcgtgacc aaggaaccga atggcccgcg aggattgctc cacacggtct cctccgtggg
      241 ttcgaaccaa acgcgcctcg ctgctctcgc gaaccgcgag ttacagctct ccgtcaccca
      301 attctggaac ccgaacacga cgacgggagt ttacaacaat caccccgtac gaacgagatt
      361 cgacgggagc agctggatca tcgagaccac cgatggtggc agtttggcgc tgggagcggc
      421 gttcaacgtc ttgtattcgg aacccaagat tctaactctc caaagtccat ctctcattca
      481 gagtgcgccc ggtccgcttg gtgatgccca gccgtccatc gtggacacca aaatcacgaa
      541 taaggccgtc ctgaagaacg ggccctcctc gatcgggagc gacaagctgc ctccgttctt
      601 cacgagcggt aagtacgcgg aactgactga ctcgctgagc ccgtcgtcaa agacagcagg
      661 tgacccgccg cccgaggagt gtccgccaga cacgggcgaa caccgggccc aaccgggagt
      721 gctcgtgtcg gagcggcgct atatcgatca cgtcgccagc tcggacaatg ccctctacaa
      781 ctatacgtgc attccggccg actccttgct gacccgcgat gccctgctga tcgtcgaacc
      841 gggagagacc acgcgcgacg aggaacgtgc gctcggtgtt tggtaccgga gcccggagca
      901 cggcttgggg tcatggtgca tcttttatca agatgtcaat ctgaccatgg cccccggaac
      961 gcgcttcttc gtgcaggtct ggacgcccga gtgaggctgc gcctccaagt atttcgactc
     1021 cccgctgtaa gacctgaccg cgaagagaac tcatatcgat agaccttgca gttcggaccg
     1081 gagccgctcc ccaggcgact cgctaattgg cgcgtgcgca ggtggacgaa agacggagag
     1141 ctgcatgcca aatatggaaa gaagggaaac aagaccatga agaacctcct acgctcgctc
     1201 gtgattacag ctgggctcgt gtcttttgcc ggtgccgctt gggcgggtga ttaccatgcc
     1261 tgcaaagtga ccgacgttca gcacgtgaag gtcggtacga ccaactactt cgtattcaaa
     1321 gcaaactgct cggatgggac gtacttttgg tcggcgccca cggatgcaat cgggacgccg
     1381 ctcatgagca gcctggtcac gacggcgttt acgagtggac gcctgatcaa cgtgcaatgt
     1441 ggcatgaacg gcggcagctg cggcgcgttc tctaccaagg tatacgatgc caacgtccac
     1501 accacgcaaa cggtgtggac cgcagaaacg gtaaacctgc gatagtagcc agcaccgggg
     1561 tcagcgccct tccaacgcag cggtttgcgc gcgtgtttgg cggtgctcgg gatggcgcga
     1621 gggccgggcg ttgtcagcgc ctcgactcgc gccagaccgc ctcggccccc tcaacgtgtg
     1681 tcgaccagga agctggccca agtcgcgatg ccgcgattgg ggcaacgctg gctggccaag
     1741 cagcggctga cttcttgtac gtgtcggatg atatcgagtg acgattccat acccagcagg
     1801 gctcgcgaga agtcctcggc atcgcggtcg agcaccggcc acaaggcgcc aatgacggtg
     1861 cgagagcccg agagcaccaa tgctcgcgcg aggccaatcg cttcatcgct gtagctcgat
     1921 cgcccgcgcc acaatcgagc agtctcgcag ccgagcaaca cgaccgtgct cagccgattc
     1981 cgtagttgca tcaagtcgcg ggcctcgacc cgatcttccg gccatgccga cgtgtcggct
     2041 tgctttgccg aggtgtagag ggcgctgcgc aaaggccggg ccggatcgta agaggcgtgt
     2101 cccgcaaaga gggcggcgtc ggccgcaccc aaggatgaca atagagcggt gcgggtggct
     2161 tcacgctctc ccaaaagcgt caccctcgtg aacgcagacc gagctcgttc gatcaacgga
     2221 tcgaaaccgg gcaacggggg gaaggtgggg tgcctcgcat tgacggcgag cacccagttc
     2281 gtctgattgc cgtgctcgaa cactgtttgc gcgtcacgcc agcccgcttc ggctagggtc
     2341 aacggcagca tccgcgctat cccaaagcgt tcgatgagga aagagctgcc gtcatacaat
     2401 gctgcccacg ggagggtgtg caggcgtaga tgcggagaga tgaacaggcg ggtcttgcct
     2461 tggagttggc tctgcaccgg ttcaatcagg gtagcatgaa gccgtttgcc aacttctcgc
     2521 gcggcaaact catcgttgtg ctcgacgcca gccgcgagcg tcaccacgtc atccagtaat
     2581 gtctcgggct gctcgacgag ggtcatgctc gccccctcac tcgtaagcac cccgacggcg
     2641 atttccggtc tccgctggga attggcgccc aagaacgtat acgacaagac ggcgtcactc
     2701 tgctcgagtc gccgagccat ctcttccacc gtcccgagtt ctcgcgcaaa cgggtctcct
     2761 ctgcgcgatt cagggtcggg ggctgtaccg gtggaacgag ccggttccag atgcaggtcg
     2821 cgcatcgggg agatggcgcg agctccggac gccgtggatc cccgtaacaa agcaaaaagc
     2881 ccgcggccct tgcccgcttc ggcgagcgcg atcgcgtcta cgccgagatt ggcgacggca
     2941 ccgaggcgcg cggcagcacc gtagtaaccc agtgcgttgg tgagcagcgc ctcacggtcc
     3001 gccacgtcga cctcgagcgc tagcaagaac gtcacccact gtgcggcacg tttcgcggca
     3061 atcaacgctt cagcggggag cccacgctct gactgctgca gcgcagtgct gaaccacact
     3121 tcgcgctgct ctcgggggag tattgcatca gccaattgcg ctcgagcacg ctcgaggaat
     3181 cgctcactct cttccaaatt cgatgcgtgg cgggccgcgt cggccgcggc aatcatgcaa
     3241 taggcctcgg ccgcgcggtg cggctgtgcc agttctaaac cgcgtttcac ccaacgctgc
     3301 ccaagcgtcg gctcggtctt ggaaatgctg gtggccgccc gacaaagcgg ggctgcgagc
     3361 tcacgccatc gtgattcgga cggcggtagc ccttcagcgt gctcaatcaa acgacgcgcc
     3421 gcggcggccg ccctgggagg ggcggtggaa aaccgatcgt ggagcagggc cagccgaaaa
     3481 tcgagtgcgg cgtccaacgc ggattctatg ggtgccgacc gttcgccttc ggtcttgcgc
     3541 aagagctccc tctccgcggg aggatccgcc gaaacatcgc cttcggctcg cgccaggatc
     3601 tgcactcggc gaagccgcgc gttccagtgc ccgcctttcc acttggcggc gagtccagtt
     3661 tccgagaggc gcctggccag cccgattcgg cccaatccga aggctgcttc ggcgccaata
     3721 cctgctgctc gagccaagtc cgaggaagga tattggccgc tcgcgccttc gacgcgaacc
     3781 agcgaggcca tcagatctcc gtgttcgagt gccgcttccg ctgcatcgag aacggtcgat
     3841 gagtattggg gctccaagtt tgggatccga acggatcgga tgcttgaacc gaggtcgaag
     3901 tcctgaatct tctcgatcgt ctcctttagc gacagctctt tcggagcggc cgtggctagc
     3961 tgccttaagg cagattcgag ctgacctttg cgaccatatg ccagcaaggc tcgtgttgct
     4021 ccggtcagcg ggcgtaagga gcgaagcggc aacgagacct ctacgtccgc aagccgttca
     4081 aacggcaatt ccgcagggag cgcgggccgc acgctttgac cggtggagtc gaaaaggaag
     4141 aggaccgcga actgacctgg tcccaagccc tgaatcctta cctccaaaat cgaatctatg
     4201 gggctgcgtt cgggcgaatg cgtgggtggg tgagagccca tggccagcac ggggatcgcc
     4261 cgtccctcga aatccttgaa ggtgacatcc cagcgcaggg cggggcgtgt ggtcacctca
     4321 tcacgaatcc gccattgaaa cagcacgact gcggcaagta cggcgagcgc tattaaccca
     4381 agccaatttg cggccgggcg gcgccgaatg aacggcttgc acttgaacgc aggtagacgc
     4441 atcggccgca gcaatcgata ttttgaagca cttccgcgtt gcgcgcctcg gtctcttctc
     4501 aaaacgtcgc actgggcacg gtgaaacgcc gacgcagctg ctgggcagct cggtcggctc
     4561 cgcccgtcgg ctcagctctg tcggctcagc ccggtcggcc gccccttgcg tccatgactc
     4621 gaaaattgac tacctgttcc actcgaacac tccttcagtc agcggacggc cgccaagaag
     4681 agcgattgac ggagtgctag ggacatgtaa attcgcgagc tcggaggcag tatgaaggcg
     4741 agactcaata gtccccagcg gggtccatgg gtgtttacgt ccgtgttgct tgctttggcg
     4801 gcgccagggc tcacgcagtg cggctcacaa gacggtgatt cgggcgcaaa tcaactcgat
     4861 cacgtcgagg aggaggctgc aaagcaaacg tccccgctcg gcacgttcaa cgtgctcacg
     4921 cgcagctaca acaatcaacg taccggcgcg aatctgagcg agacgcagct cacgccagcg
     4981 aacgtgaact cacggcaatt cggcaagctg tttcaactgc cggttgacga cgaggtttac
     5041 gcgcagattc tttacgcctc gggcgtaagc atcggcggtg cgacccggaa cgttatctac
     5101 gtggcgaccg taaataacac cgtctacgcc ttcgacgcgg atacggccgg agcgccgctc
     5161 tggcagcgca acttcaacgg tagcggccgc ccgccgcgtc atggcgaagt ggccaacggt
     5221 acagcctgcg gaggcaacta tcacgatttt gccggaaaca tcggcatcgt ggggacgccc
     5281 gtcatcaacg cgagctccaa caccatgtat ttcgtgaccc gcacgctgga gagcgggagc
     5341 atgatctacc gcttgcgcgc ggtgaacatc gcgaccggcg ccgatcgcgc cggcagtccg
     5401 aagcagatca ccgcctcggt agcgggaacc ggagatggga gcaatggcac gaccatcgcg
     5461 ttcaatccga tgttccacaa ccagcgcgcg gggctcgcct tggcctcgaa tgctgtctac
     5521 gtcgcgtttg ccgcctattg cgacgcgccg ccgacccacg gctgggtgat gggcttcgat
     5581 gcgacctctt tggcctcgac gggcgtgttc aatacca
//
LOCUS       DASTKU010001083        19073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1776807, whole genome shotgun sequence.
ACCESSION   DASTKU010001083 DASTKU010000000
VERSION     DASTKU010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 19073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19073
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776807"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3629)
                     /locus_tag="VFQ61_38650"
     CDS             complement(<1..3629)
                     /locus_tag="VFQ61_38650"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9960473.1"
                     /translation="MTTSNAPVRSSPDPASFVAAVARLRAEQEVASTSQAQALLFHEM
                     GVLEERHGDEAAAARDHLAAVNCDPDFREPLERLIAIVERRQSYKNLGKLLERLVRVA
                     ETPTERARALVDEATHFADFENNFDGARSSLQEAAENAPEDVTLWLALERVAARMADP
                     SLSERALTSRAELVQDPTLRGLLLADIAEGRAAQGELDGAFEALDRAIGQGGEATFLA
                     MLAEERIAREHGRADVEAKVLEMQGELVLMAITDAQAGDTYGVPRHKRHPGAVGDAWL
                     RAAELYRRQGDTAKTTTLLERAISELASEPSILHARLCLAEASSDRATAARLARAELE
                     HGMTGEVGASLWLRVAEAAAAEEQIPDALEALGKALSLAPGSIEARALYLDLLAARGE
                     PGQLAAALEATAEQLELDSAKANYYLAAADVWARLAKDVSGAKAALSQAGMFGLSKML
                     SARAARMLASLLGDTTWYEEATRRLLAQGATEEEQADLWLELVRLRLLRKDTESARHA
                     LNSAAQTPSGAFLSRLLAAYALPWTQSGDTPAPVDPGGATLFTELAELEPEPELAQGL
                     KVISARRALERGELARATETLSALQREDGSDLILTGALSAAFAAQDQAAKVRDVFEQA
                     AQNCHDPELAAALHAAAFVLSWKVGERARALSSLQKASDEQPSTFGPLLGSALRAAGA
                     DDASARERAHEALASADPALAALERFGLEVGADGSETTAWEALTELRVATDSELGRAG
                     ELARALWAPTGGDGASRREALEGLAEHGREAARLAQALLHQLTLSEEGDLPDPSRVLE
                     SAERLARADSSAVSALEWLAAAIAAQEHDQEIQARLALAARVTEPLSSAVSSSAALMA
                     ELLGTGENASVRSDSPAARLTNLELAVPGQDPGRRAHALAAAAPLIGEDSEPLVLALA
                     GFNQLAAGDIQGAIKSFRSVVDTYPDEVIGWEGLRAAALADGDRATLAEACAALGDAV
                     SDAGLGAKLWEEAASILLDELSDPVRGEFALTRATERDIGLFSAFDRLFRILRARKDA
                     PRLLGLIEQRLTVASDPQEVLRLVWERARALRESGDREGALAALQKVRELEPDHVGAL
                     ALTGEICITLQRYEEAAEHLARLAAHPSAPQQQRLMSGVAAVDLYESRLKNPNRALEV
                     LTGLYRGGLSTLPVRERLARTATRVGAYDEATEVLEVLMEERETPAGRIEAARLAMVI
                     YRDELG"
     gene            complement(3877..4467)
                     /locus_tag="VFQ61_38655"
     CDS             complement(3877..4467)
                     /locus_tag="VFQ61_38655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET9960474.1"
                     /translation="MQKNTALAVGFLIVIGAVLVIVMGGNRGQSHPAAPATATVAAPS
                     AAPSAPIPSASAIAPTPSAAGLDLEATDALTSEGFDVLPDGRKAPPLPASAPKEISFG
                     VALFTYEGAEGAPKNAPKKEDARKHAESLIAEAKQDFAAVVAKGDPGSAADKGKMLRD
                     TLEPAVEYQLFSLEKGAVADHPIDTPKGFWIARRNR"
     gene            4978..10155
                     /locus_tag="VFQ61_38660"
     CDS             4978..10155
                     /locus_tag="VFQ61_38660"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9960475.1"
                     /translation="MFKVECPGCKAPYQVDERRVPANGLKMRCPKCGSSFKVDPPADV
                     VGALAGAAPSNADPTSSNPAQSQLAQSQAASGAHRPPVRRNSGLKATMLGVAPATGRN
                     VPAAPPTGVAAPQNAPTNAAATPGAAPFRAEPAVPRNVAGANAGGALTPEQGSLGRPG
                     APDPVMNPSPVMNPSPVMNPSPVMNTAQRPGSPPAPKAPGVAPLGGASPPGGVGDADL
                     PGLVKPAALNTGTKPPSTPHAAPPVSRATMPLGVPAPLGVPAPGAPASVGVAVQAGVA
                     ASVDLPAPVATSAKAGGFGAPRPPARPAPAAPSPSPEVPGFELDLPDLGQNGRKPAPP
                     VAAAPGAAASPRQPTLDADLPSVSGGRSGPKAKNDFGDLGLELPSKVEKPAAAKAPGI
                     APVPPAKGPAAPVKAQPSPTTKTAADLPAQRAAVPSPAAKNMSRASTPDREGPALDLP
                     SPRGVIQPELDAGLPAVSAAALPSLSSGRAATVNERARAPTLGGAFGKPAQASLERPR
                     ANTLTGAPDLEPPKPSPALLDDLPSLADDASGLPALSGMGADSDPALPAVSYSELPSA
                     LGGPGLERGALRAPEPRAIDLRDSGLPSALTTGTQGARDVPAPGRRNVPDIDLPSVSL
                     SGLPSALGPESLPSISDPALPRSSGEAGLPSPLGGLSLPSLGGPGLPTPASAGLPSPG
                     GGSTPGLPSVLGPSVRDFGPLELDAPRSPPTSGFGELELPPLSMDKPERPRVALDSDP
                     FGEAALPPPRSVDREPRANREPAVGYGPGPALEAPLSAGSLIGPDSGGVSAAATRGVA
                     GSGVAGGGVAGGSHAPGVLSPRAMPGVGAVVRQSGGGTAYGEVNLGGDDELDVPLEAP
                     APAVTSSATRPAQASDEDMEFGGVPQEAAKAPAPMARAAAVQSLPARAPAVVRGAPAR
                     GQPKKTGRTGLKVLGIFAVLSIGGAALAFDPRLGPFGANFIIDRVRADDYQRAVDLSV
                     AHTRTALQRDTARSAAQAAAELDSERARLPRVRALAAYSAYLASLRELRFGAEPELHA
                     RAKVLLDELATTSEVPYLELARAARSATDGQLARARQELNGALRRNPKDIDTLVLSGE
                     VELRARDAKAALAVWQTLSAVEQSARAGFGLARAQFASGASAAADAAAKRVLEKNPDH
                     VGAKILRARVSALTSPRDPAAVTLLEGIVKNPESASSEELVRAHTLLGDVHLSRSRMS
                     RAEASYTEALKINPKAAGALIGLGEALYRAGRYSESQARFEAGVQADPDDVFAKVGVQ
                     KCKLALERVEEAHAALKKLLESHPASPLVAYWYGRVLETMGSRDEAERTYKDAIQKGQ
                     TDPLLGDIYISLASLQNQVGRAEAANKTLAEARERMPDSPAIHRALADVALSQGRYSD
                     AQTQLKRALELDPEDLGSKFRFAVALSRDDQVDPALRVFEEVAKIDPEYPGLALERGL
                     LLEKMGRLEQALEAYEKALAKAPGDPDLLLRVGCGRSAAGRTKEAEAQLRKVLELRPT
                     SAETHFCLGRALLIEGTRLADAFRFLERAVELDPHRAIYFCYLGWIANEAGNVPRAEQ
                     ALNKAIELDRGLADAYWQRGVLRQRQGQVRDAVQDLSKALELRPTRYEAHAALADAYY
                     DLGKEALALAELQKAVKAQPENATWHYRYGKLLGDNHMNEPARVELTRALELAEKSQI
                     AERWLWEAHRRLALVIGPRPEAIPHWEEFLRRAPPDSPYRGEAIAMLAKLGKPWTGN"
     gene            complement(10285..10689)
                     /locus_tag="VFQ61_38665"
     CDS             complement(10285..10689)
                     /locus_tag="VFQ61_38665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HET9960476.1"
                     /translation="MKTIGIAILGLCAGLAGCSFAARSPEMYRDETAALLETKSAQVK
                     ACYDETLKTNKDAQGRVTVRFVVEHETGKLTSVAADPAGTTAPEPLTKCVVDALQGLA
                     LSPPDQRDGNATFVYEFTQGQPGGAPAPAPAG"
     gene            complement(10713..11495)
                     /locus_tag="VFQ61_38670"
     CDS             complement(10713..11495)
                     /locus_tag="VFQ61_38670"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HET9960477.1"
                     /translation="MHTFRTGSQLVLSKLVLHSTLVSITVLAAACGGVSTFQGQSAIS
                     VVGTPPAPPPPPPPPPKKEEPPPPPARVEVRDNKIEINEKIQFEYNKATILQASFSLM
                     DEIADTIKKNAHIKKIAIEGHASSEGDAAHNLKLSDDRAKAVMKYIVDKGVDAGRLTA
                     KGFGAKRPIADNNTPEGKEKNRRVEFVILEQDVTQKKVEVDHTGKEKVLEEKKLTDKK
                     EEPAADAKAAPPAAAQPGAAAPKKLPALKAPPKPADKPAEKK"
     gene            complement(11756..13744)
                     /gene="rpoD"
                     /locus_tag="VFQ61_38675"
     CDS             complement(11756..13744)
                     /gene="rpoD"
                     /locus_tag="VFQ61_38675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET9960478.1"
                     /translation="MGSKKPERPAKKSAASVAKKAPAPEKPKAKLSTKASKASAPASA
                     PPSESDDADFDDDEAPSSPVASQAKVNGKTKEQLINLGKSKGFLTYDDVHEALPGEDV
                     GPDQMDDVLSALDDEEIEVVDDASNIKIAPHRVSEDEAPSKAAPKADEETTTEGPTST
                     PGRTSDDELYKSNDPVRMYLRKMGSVALLTREGEVEIAKRIEEGENEVLASILSSPIA
                     VREIIDIGERLRNHKIRVKDIVRDAEDEEHEFDEEEADRRIIRLIDRVRRLDKKNSEL
                     LEERKTAAEARRKLIDKELSDNKKELVKTLQEMRLNKKTIDKIVAKLKAMIERVYRSQ
                     GKVIELEKITGASKSELKKMLREAKDDPAAEDELARKLGVDRGDLDSMDEALKNASKG
                     MKKVEEELKIDVGELLRTYQAIRMGERKAEKAKAELVEANLRLVVSIAKKYTNRGLQF
                     LDLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIET
                     INKLIRTSRYLVQEYGREPTPEEIAEKMELPLDKVRKVLKIAKEPISLETPIGEEEDS
                     HLGDFIEDKSVVSPADAVINMNLSEQTRRVLKTLTPREEKVLRMRFGIGEKSDHTLEE
                     VGQDFEVTRERIRQIEAKALRKLRHPSRSKQLKSFIES"
     gene            complement(13755..15692)
                     /gene="dnaG"
                     /locus_tag="VFQ61_38680"
     CDS             complement(13755..15692)
                     /gene="dnaG"
                     /locus_tag="VFQ61_38680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET9960479.1"
                     /translation="MILRYASAVIGQDTLDRIRRETDLVALIGESLKLQRRGRSFVGL
                     CPFHKEKSPSFHVNAERGFYHCFGCHASGDAFKFVQEIEGLDFQEAVRRLADRAGIVI
                     DWQISDAELARQREVRRRADELYEVVNLAAAYFERMLREHPLSAYARAELERRKLVPS
                     SPTDPVADALQAFRVGYAPFGWDGLAQSLKNSGASLVAAEKVGLVAARKNAVGYYDRF
                     RHRLMFAVLDLQGRPIAFSGRALEEPPAAELRELSLAPSPPGAEKPAKYYNSPESAIY
                     KKREAVFGLYQARNAIRTQESAVIVEGNFDVLSLHAFGVKNAVAPLGTAFTLEQAQQL
                     RRFTNDVVLLFDGDEAGKRAVQGAREVCQKAALGAKVATLPTGSDPDELIRKQGREAL
                     DLILNRARGLLEYLIDGVLDETFSESDARTRAARVKKVIELLGEETDPTVRALAERHA
                     DRIAQRLNITDEPTFRALVNEVRRALDRDAAGVSAPKKELVAPPVRARSPDRRDSIGL
                     EILGVFLDFPQLFEDPEASSAGELLEGDTAIAFAALRQLSKAGAISNPEQVLAKLPPS
                     IHQFARARLVAPRHSRTEDAQSELSGNIRQLKALVLRRDKRNVVEELERARRTGDFEQ
                     EQELLKQLFSRARQHRAVVSE"
     gene            16153..17289
                     /locus_tag="VFQ61_38685"
     CDS             16153..17289
                     /locus_tag="VFQ61_38685"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HET9960480.1"
                     /translation="MLRRLPVANPQNRWERAHVSYESGDIPNQGLQLFLDDSRRVISE
                     NDSPDVSFRFSLNPYRGCAHGCAYCYARPSHEYLGFGSGTDFERRLLIKPRAAELLRE
                     AFEAKGWLGDLLVLSGNTDCYQPIEAQLGLTRGCLEVCVEYRNPVHIITKGSLVERDL
                     ELLVRLHEVASVSVSVSIPFWDPDAARSMEPYAPPPARRIETIRRLSSAGIPVVVNVA
                     PLIPGLVDADMIPILEAARAAGALSAMSIPVRLPGSVAQVFQERLHAHFPGKAEKVLA
                     RIREMRGGALNEPRFHERMRAKGEYGATLFRVFQATCTRLGFGEFPEPRIGTFRRPKA
                     AENGDNRARARRQTARVNPPLGSTQLSLDFSATARNTSDEGNAP"
     gene            17407..17946
                     /gene="atpC"
                     /locus_tag="VFQ61_38690"
     CDS             17407..17946
                     /gene="atpC"
                     /locus_tag="VFQ61_38690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HET9960481.1"
                     /translation="MAEQLSLEIVTPDGVKLSEQVSELTAPSVNGEFGVLPGHRPMVA
                     ALTTGIVSYVHGGEKHAVAVGAGFVEVADDKALLLTDRFVRKADVDPVLVRKELKEVD
                     EALDKLESDQSSSEYGSLVARQLWAATQLDLYGDPPPPRLRTMNELMFAQHETYADLT
                     SDVASGEEGTEKHADSETH"
     gene            17965..18810
                     /gene="rsmA"
                     /locus_tag="VFQ61_38695"
     CDS             17965..18810
                     /gene="rsmA"
                     /locus_tag="VFQ61_38695"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET9960482.1"
                     /translation="MEPPQSPKAVLAQRGLSPKHHFGQNFLVDRSIIERIAALATPRP
                     GTVIELGAGLGALTRALLERANLVIAIERDRDLIPLLEQDFSIDLGAGRLRLLECDAK
                     SIEPAALFAEGQPPYTLCGNLPYQITGPLMRLAMAHARSFERAVFLVQLEVAQRLAAG
                     PGSEDYGALSVFAQASFNVKREFVVRRGAFFPQPRVDSQVVSLQPRTDPIAETSLFSN
                     LVSAAFEKRRKQLGNAWRGLPGVDHSRIVKAAELAGIDLQARGETLSGSDFHRMERAI
                     EELAR"
     gene            18807..>19073
                     /locus_tag="VFQ61_38700"
     CDS             18807..>19073
                     /locus_tag="VFQ61_38700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960483.1"
                     /translation="MRNGSGALLLSCVLLASSCRRGTDRNTEPRIEFGVLFGGDIQDR
                     PVIPLELDASRQELALRVTFPEPVQRERRVRFELERPPAPNREKG"
BASE COUNT         3197 a         6191 c         6059 g         3626 t
ORIGIN      
        1 ccgagttcgt cgcggtaaat caccatcgcg aggcgcgcgg cttcgatgcg cccggcgggc
       61 gtctctcgct cctccatcag cacctcgagc acctcggtag cctcgtcgta ggcgccgacg
      121 cgcgtggcgg tgcgcgcgag ccgctcgcgc actggtaacg tactcagccc tcctcgatac
      181 aggccggtca ggacctcgag cgcacggttc ggattcttga ggcggctctc gtacaagtcg
      241 accgccgcca ccccgctcat caaacgttgt tgctggggag cactcgggtg cgcggcgagc
      301 cgggcgaggt gctccgccgc ttcttcataa cgctgaaggg tgatgcagat ctcgccggtc
      361 aaggcgagcg cgccgacgtg atcgggttcg agctcacgca ccttttgcag cgcggcgagg
      421 gccccttcgc gatcgcctga ttcgcgcaga gcgcgcgcgc gctcccagac caatcgcagt
      481 acctcttgcg gatcgctcgc taccgtgagg cgctgctcga tcaagcccag cagacgaggc
      541 gcatctttcc gcgcgcgcaa gatgcggaat agccggtcga acgcgctgaa cagaccgatg
      601 tcgcgctccg tggcgcgggt cagcgcaaat tctccgcgca ccgggtccga cagttcgtcg
      661 agcagaatgc tcgcagcctc ttcccacagc tttgccccga gaccggcgtc gctcacggcg
      721 tcacccagcg cagcgcaggc ttcagccagc gtggctcgat cgccatccgc cagggccgcc
      781 gcgcgtagcc cttcccagcc gatcacttcg tcgggatagg tgtccaccac gctacggaag
      841 gatttgattg ccccttgaat atcccccgcg gcgagttgat tgaaccctgc cagcgcgagc
      901 acgagcggct cggaatcctc cccgatcaag ggcgccgcgg cggccagagc atgggcacgc
      961 cgcccaggat cttgcccagg tacggccagc tctaggttgg tgagacgcgc ggcgggagag
     1021 tccgaacgca cgctcgcgtt ttccccggtt cccaaaagtt cagccatcaa ggctgcactc
     1081 gaagagaccg ccgaggacag tggctccgtc acgcgcgcgg ccagcgcgag acgtgcctga
     1141 atttcctggt cgtgctcttg cgccgcaatc gcggcggcga gccattcgag cgcggacacc
     1201 gcactcgaat ccgctcgcgc caggcgttcc gccgactcca acacccgact cgggtcagga
     1261 agatcccctt cttcactcag ggtgagctgg tgcagcagag cctgggccaa ccgggcggct
     1321 tcacggccgt gctccgcgag cccctcgaga gcctcgcggc gtgacgcgcc gtctccaccg
     1381 gtgggggccc acaaggcgcg cgccaactcg ccagcacggc cgagttccga gtcggtcgca
     1441 acgcgcagct cagtcagcgc ctcccaagcg gtggtttccg agccgtctgc gccaacttcg
     1501 agtccaaagc gttcgagggc ggccagcgcg gggtcggcag acgcgagcgc ctcgtgcgcg
     1561 cgctcgcgag cgctcgcgtc gtctgcgccg gcggcgcgca gcgcactgcc gagcagggga
     1621 ccgaaggtcg aaggctgttc gtcgctagcc ttctgcagcg acgaaagagc acgcgcgcgt
     1681 tctccgactt tccacgacag gacgaatgcg gcggcgtgga gcgccgcggc cagctcggga
     1741 tcatggcagt tttgggccgc ttgctcgaaa acgtctcgga ccttcgccgc ctgatcctgc
     1801 gccgcgaacg ccgcggatag cgcgccggtc aaaatcaagt cggagccgtc ctcgcgctgt
     1861 aacgcgctca gggtctccgt ggctctcgcg agctcaccgc gttcgagcgc tcgacgagcc
     1921 gagatcactt tcaagccctg agcgagctcg ggctcgggtt cgagctcggc gagctccgtg
     1981 aacagcgtcg ctccgcccgg gtccacgggc gcgggggtgt caccggactg tgtccagggc
     2041 agagcgtagg ccgcgagcag ccgtgagagg aatgcgccgc tcggcgtttg ggccgcggaa
     2101 ttcagcgcgt ggcgcgccga ctcggtgtcc ttgcgcagca gccgtaagcg cacgagctcg
     2161 agccacagat ccgcctgttc ctcttccgta gccccctgcg cgagcaggcg ccgcgtcgct
     2221 tcttcgtacc aagtcgtgtc gccgagcaaa ctggccagca tgcgagccgc gcgcgccgac
     2281 agcatcttcg acagaccgaa catgccggcc tggctcaggg ccgcctttgc gcccgagaca
     2341 tctttggcga gccgcgccca aacgtccgcg gcggctaggt agtaattggc cttggcggag
     2401 tcgagctcga gttgctctgc ggtggcttcg agtgcggccg ccagctgccc gggttcaccg
     2461 cgcgccgcca gcaaatctag gtaaagggcg cgcgcctcga tcgagcccgg tgccaggctc
     2521 aacgctttgc cgagcgcttc gagggcatcg ggaatctgct cctcggcggc agcggcttcg
     2581 gcgacacgca accagagcga agccccgacc tctccggtca taccgtgctc gagctccgcg
     2641 cgcgccagcc gcgccgcggt cgcgcgatcc gagctcgctt ccgccaagca cagacgcgcg
     2701 tgcaggatgg acggctcgct cgcgagctcc gagatggcgc gctcgagtag ggtggtcgtc
     2761 ttggcggtgt cgccttggcg ccggtatagt tccgcggctc gcagccaggc gtcgcccacc
     2821 gcgccggggt ggcgcttgtg gcgtggcaca ccgtaggtat ctccggcttg tgcgtccgtg
     2881 atcgccatca gcacgagctc gccctgcatc tcgagcacct tggcttccac gtccgcgcgc
     2941 ccgtgctcgc gggcaatccg ctcctccgcg agcatcgcca ggaacgtagc ctccccgcct
     3001 tgaccaatgg ctcgatcgag cgcttcgaac gcgccatcta gctcaccctg cgcagcgcgc
     3061 ccttcggcga tgtccgccag cagcaagccg cgcagcgtgg gatcctggac gagctcggcg
     3121 cgcgacgtca gcgcacgctc gctcaggctc ggatcggcca tgcgcgcggc gactcgctcc
     3181 agcgctaacc aaagcgttac gtcttccggt gcgttctctg cagcttcttg taaagaactt
     3241 cgagcgccgt cgaagttgtt ttcgaagtcc gcaaagtggg tcgcttcatc gaccagggcg
     3301 cgagcgcgtt cggtgggcgt ttccgccacg cgcaccaggc gttccaggag cttgcccagg
     3361 ttcttgtatg attgtcgccg ttcgacgatg gcgattagcc gttcgagtgg ctccctgaaa
     3421 tcggggtcac agttgacggc ggctagatga tcacgcgccg cggccgcttc atcaccatgc
     3481 cgttcctcga ggactcccat ctcgtggaac agcaaggctt gggcctggct cgttgaagca
     3541 acttcctgct cggctcgcag ccgcgcaacc gcagccacga aagaagcggg gtccggcgaa
     3601 gagcgcacgg gcgcgttcga ggtagtcatc ccagagaaac tcacggcgaa tctagaggac
     3661 gtgaacgcgg gtgcctagcc cctccgttca ttttcgagac cgttcgtttc ccggttcgcg
     3721 atcacgctgg cggcgctcta gagggccgcg cggacccttt agagcgcaaa gcgctgtttc
     3781 tctagaactc tccaagcgct gtctccccga gcgctcggcc cgttatctcg ctcaccgagt
     3841 gtgagctttg gctacttcag aatcgtcgtt cctgacttat cgattgcgtc gtgcaatcca
     3901 gaaacctttc ggcgtgtcga tgggatgatc ggctactgcg cccttttcca agctgaagag
     3961 ctgatattcg accgctggtt ccagcgtatc gcgcagcatc ttgcccttgt cggcagcgga
     4021 gcctgggtcc cctttggcca ccacagcggc aaaatcctgt tttgcctcgg cgatcaaaga
     4081 ctcggcatgc ttgcgagcat cttctttctt gggtgcgttc ttgggcgcgc cttccgcgcc
     4141 ttcgtacgtg aaaagagcca ctccgaagga gatctctttg ggcgcgctgg cgggcagcgg
     4201 aggagctttc cggccgtcag gcagaacgtc gaacccctca ctcgtcaggg catccgtagc
     4261 ttccaaatcc aggccggctg cgctcggtgt gggagcgatt gcagatgcgg acgggatggg
     4321 cgccgaaggc gcggcgctcg gggccgcgac cgtagcagtt gcaggcgcgg ctgggtggct
     4381 ttggcctcgg ttcccgccca tgacgatgac cagcacggcg ccaatgacaa tcagaaagcc
     4441 aaccgccagg gcggtgtttt tttgcatgga aagctcgctt gcagttctct aggccaagga
     4501 tgacggtcgc ggtaaaacgc tggatctaga gggaagagga agaattcggg aatgaaggcc
     4561 aggggcgagg gattatgtcg caggctctca cgcgacgtcg cggaacatct tgcgaagtgg
     4621 ctgcatgtgc gcacggggcc cgtgcggttc gacccactat ctcagccgcc ctggccaagc
     4681 aagcatggcc aaaagcgatg gcacaaacgg acccgctacg agaccgccgc aatcagccga
     4741 ccgccgcaat cagcccagag gtgctgaggt aggggttcag gcgcacacgc tgcatgcgac
     4801 caccgggcgc gtcggcgtgc gagcctcgag ccacgcgata ccatcaggca aagcaagcca
     4861 gccgagcaaa gcaaagattc caagcctgcc gagcgtacga gggtcgagca gacccgctct
     4921 ggcagaaact gggacagagg gaccgctctc ctgctagaag tcggaagata tcgttcgatg
     4981 ttcaaagtcg agtgccccgg gtgcaaggcc ccgtaccaag tggacgagcg ccgcgttccc
     5041 gcaaatggat tgaaaatgcg ctgtcccaag tgcggcagct cattcaaagt cgatccacct
     5101 gcggacgtcg ttggtgcact agcgggagcc gcgccttcga atgccgaccc tacgtcgtcc
     5161 aaccccgcgc agtcccagct cgcgcagtct caagccgcat caggggcaca ccgcccgccg
     5221 gtccgtcgta attcggggtt gaaagccacg atgctgggtg tagccccagc cacggggcgc
     5281 aatgtccctg cggctccccc gaccggcgta gcggccccgc aaaacgctcc aaccaatgcc
     5341 gcagctacgc cgggtgctgc cccgtttcgc gcggagcccg cagtgccacg gaacgttgct
     5401 ggtgcgaacg caggtggggc gcttactccg gagcaaggca gtcttggtcg ccccggagct
     5461 cccgacccgg tgatgaaccc cagcccggtg atgaacccga gcccggtgat gaacccgagc
     5521 ccggtgatga acacagcgca gaggcctggc tcgccgcctg ctccgaaggc acccggtgtt
     5581 gcgcccttgg gcggagcttc accgccgggt ggggtagggg acgcagactt gcctggcctc
     5641 gtgaaaccgg ctgcgttgaa cacgggcacg aagccgccca gcacgccaca cgccgccccg
     5701 ccggtctctc gcgcgaccat gcccctcgga gtaccggcgc ccctcggagt accagcgcca
     5761 ggagctcctg cgtcggttgg agtggcagtt caagctggcg tcgcggcttc ggtcgacttg
     5821 ccggcgccag tcgccacttc ggcgaaagca ggcggcttcg gggctccaag gccacccgct
     5881 cgtccggctc cggctgcgcc atcaccttca cccgaggtcc ctggtttcga gctcgattta
     5941 ccggatttgg gccagaacgg gcgcaaacct gcgccgccag tcgcggccgc tccaggagca
     6001 gcggccagcc cgcgccaacc cacgctcgac gcggacctcc catcagtgag cggcgggcgc
     6061 tccgggccaa aagcaaagaa tgacttcgga gacttgggtc tcgagctgcc cagcaaagtc
     6121 gagaagccag cagcggcgaa ggcaccggga atagcgcctg tcccgcctgc aaaagggccg
     6181 gctgctcccg tcaaagcgca gccgagtccg acgacgaaga cggctgccga cttgccagct
     6241 cagcgtgccg cggtcccgag tcccgccgcg aaaaatatga gcagagccag tacgccggac
     6301 cgggaagggc cagcgctcga tctgccttca ccacgcggcg tcattcaacc cgagctcgat
     6361 gcgggcttac ccgcagtttc ggcagcggcg ctgcccagtc tttccagcgg tcgagccgcg
     6421 acggtcaacg aacgcgcgcg cgcaccaacg cttggtggcg cattcggtaa accagcccaa
     6481 gccagcctag agcgccctcg agccaacacg cttacgggtg cgccagatct agagcctccc
     6541 aaaccttcgc cggctctgct cgatgatcta ccaagcctgg cagacgacgc ctccgggttg
     6601 ccggcgttga gcggcatggg cgcagactcg gatccggctt tgcctgctgt cagttattcc
     6661 gagttgccat cggcgcttgg cgggccggga ctcgaacgcg gtgcactgcg agcgcccgaa
     6721 ccaagagcca tcgacttgcg tgactccggg ttaccgagcg cgctcaccac gggcactcag
     6781 ggcgcccggg atgtccccgc gccggggcga cggaacgttc ccgatatcga cttgccgagt
     6841 gtgagtctgt cgggtttgcc cagtgcgctt ggtccggaga gtctacccag catttccgat
     6901 ccagctctgc cccgcagctc cggcgaagcc gggttaccca gcccgctagg agggctcagt
     6961 ttaccgagcc tgggtggacc cgggctaccg acccccgcga gcgccggtct accgagcccc
     7021 ggaggcggat ctactccggg cctaccgagc gtgctcgggc cgtcggtacg cgatttcggt
     7081 ccgctcgagc tcgatgctcc tcgctcgcct ccaacctcgg gttttggtga gctcgagttg
     7141 ccaccgctct cgatggataa gccagagcgc ccacgcgttg cgctcgactc ggacccgttc
     7201 ggggaggccg cgttgccgcc gccgcgctcc gttgacagag agccgcgtgc caacagagag
     7261 ccagcggtag gatacgggcc ggggccggcg ctcgaggctc cgctcagcgc cgggagcctc
     7321 attggcccgg actctggcgg cgtctcggcc gcggccacgc gtggggttgc aggaagtggg
     7381 gtcgcaggag gtggggttgc aggagggtcg catgctccgg gcgtgctctc accgcgggcg
     7441 atgcccggcg ttggtgctgt cgttcggcag tcgggcggcg gcactgctta cggggaggta
     7501 aatctaggcg gggacgatga gctagacgtg cccttggagg cgcccgcgcc cgcggtgact
     7561 tcgtccgcga ctcggcctgc ccaagcgagc gacgaagaca tggagttcgg tggtgtgccg
     7621 caagaggccg ccaaagctcc agcgccgatg gctcgagcgg ccgccgtgca gagcctgccc
     7681 gcgcgcgcgc ctgccgtcgt tcgtggtgcc ccggccagag ggcagcccaa gaagacgggc
     7741 cgaactggcc tcaaagtgct cgggattttc gccgtgctgt cgattggcgg cgcggcgctg
     7801 gctttcgatc cgcgcctcgg gccgttcggt gcgaacttca tcatcgatcg cgtgcgcgcg
     7861 gacgactacc aacgcgccgt agacttgagc gtcgcgcaca cgcgcacggc attgcagcgg
     7921 gacaccgcgc gcagcgccgc tcaagctgcg gctgagctcg actccgagcg tgcgcggctg
     7981 ccccgcgtgc gcgcgcttgc ggcttattcg gcgtacttgg cttcgctgcg tgagctacgt
     8041 ttcggcgcag agccggagct gcacgcccgc gccaaggtct tgctggacga gctcgcgacg
     8101 acctcggagg tgccctacct ggagctcgct cgcgccgctc gctcggctac cgacggccag
     8161 ctcgcgcggg ctcggcagga actgaacggc gccttgcgcc ggaacccgaa agacatcgac
     8221 accctagtgc tgagcggcga ggtcgagctt cgggctcgcg acgccaaggc ggccctcgcg
     8281 gtatggcaga cgttgtccgc ggtcgagcaa agtgcgcggg ctggctttgg gctcgcgcgc
     8341 gcgcagttcg cctcgggggc gagcgcggcg gcggacgccg cggccaagcg cgtgctcgaa
     8401 aagaaccccg atcacgtggg cgccaaaatc ttgcgcgcgc gcgtttctgc cctcacttcg
     8461 ccgcgcgatc cagcggccgt caccttgctg gaaggcatcg taaagaaccc tgagtcggca
     8521 agctcggagg agctcgtacg ggcccatacc ctattgggtg acgtgcacct gtcgcgctct
     8581 cggatgtcgc gcgccgaggc gtcctatacc gaggctctca agatcaaccc caaggccgcg
     8641 ggggccttga tcggtcttgg agaggctttg tatcgagctg ggcgctactc ggagtcacaa
     8701 gcgcgcttcg aagcgggagt gcaggccgat cccgacgacg tgttcgccaa ggttggcgtg
     8761 cagaagtgca agctcgcgct ggagcgcgta gaagaggcgc acgcggcctt gaagaagctg
     8821 ctcgagtccc acccggcctc gccgttggtt gcctattggt acgggcgcgt gctcgagacc
     8881 atggggagcc gtgacgaagc ggagcgcacc tacaaggacg ccattcagaa ggggcagacc
     8941 gatcctctgc tcggcgatat ctacatttcg ctcgcttcgt tgcaaaacca ggttgggcgc
     9001 gccgaggccg ccaacaaaac actggcggaa gcgcgcgagc gcatgccgga ctcgcccgcc
     9061 attcatcggg cgctggccga cgtggcgctc tcgcaagggc gctacagcga cgcccagacg
     9121 cagctcaaac gagccctcga gctcgatccc gaggatctcg gctcgaagtt ccgtttcgcg
     9181 gtcgcgctca gtcgagacga tcaagtggac ccggcgttgc gagtcttcga ggaggtcgcc
     9241 aagatcgatc cggagtaccc cggtttggcg ctggagcggg gcttgttgct cgagaagatg
     9301 gggcgcctgg agcaggcgct ggaggcctac gaaaaggcgc tcgccaaggc tcctggggat
     9361 ccggacttgt tgctgcgcgt aggttgcggg cgttccgcgg cagggcgaac gaaggaagcg
     9421 gaagctcagc tccgcaaagt tctcgagtta cgacccactt cggccgagac gcacttttgc
     9481 ttggggcgtg ccctgctaat cgagggtacg cgcctggccg acgcgttccg cttcttggag
     9541 cgagcggtcg aactcgaccc gcaccgcgcc atttacttct gctacctggg ctggatcgcc
     9601 aacgaagccg gcaacgtgcc gcgcgcggag caggcgttga acaaagccat cgagctcgat
     9661 cgaggcctgg ccgacgcgta ttggcagcgt ggtgtcttgc gccagcggca aggccaggta
     9721 cgggacgcgg tgcaggacct gagtaaggct ttggagctgc gtccaacacg ctacgaggcg
     9781 cacgctgctc tcgccgacgc ttactacgat ctcggcaagg aggccttggc actggccgag
     9841 ctgcagaagg ccgtgaaagc tcagcccgag aacgcgacct ggcattaccg ctatggcaag
     9901 ctgctcggtg acaatcacat gaacgagccg gcccgtgtcg agctgacgcg agcgctggag
     9961 ttagccgaga agtcgcagat tgccgaacgt tggctgtggg aggcgcatcg tcggctcgcg
    10021 ctcgtcattg gtccgcgccc ggaggccatt ccgcactggg aggagttcct gcgtcgcgcg
    10081 ccgccggatt ctccctatcg cggagaggcg atcgccatgc tggcgaagct ggggaaaccc
    10141 tggaccggca attgacctcc tgcgcgcggt cagagcggtc gcctcaccgc cgataaacga
    10201 aacgggcgga gagaccgcct aagctggtct cttcgcccgt cttctctgcg ctacgtgcga
    10261 gcgcggcaag gccgcgccgt gcgctcagcc agccggagcg ggcgctggcg cgccgccggg
    10321 ctgaccctgc gtgaactcgt atacgaacgt cgcgttcccg tcgcgttggt cgggcggcga
    10381 gagcgccaac ccttgcagcg cgtcgacgac gcacttggtg agcggttccg gcgccgtggt
    10441 gcctgcggga tcggcggcca cactcgtgag cttgccggtc tcgtgctcca cgacgaagcg
    10501 cacagtgacg cgcccctggg cgtccttgtt ggtcttcagc gtctcgtcgt aacaagcctt
    10561 cacctgcgcg ctcttcgtct cgagcagcgc cgcggtttcg tctcgataca tttccggcga
    10621 gcgcgccgcg aacgagcagc cggcgaggcc ggcacaaagt ccaagaatgg caattccaat
    10681 ggtcttcatg gggttctccg ttcggttcgc ggctacttct tctccgccgg cttgtcagca
    10741 ggcttcgggg gcgctttcaa cgcggggagc ttcttcggag cggcggcacc gggttgggcc
    10801 gctgcgggcg gggctgcttt ggcgtcggcg gcaggttctt ccttcttgtc cgtgagcttc
    10861 ttctcttcga gaaccttctc tttgcccgtg tgatctactt cgaccttctt ctgcgtcacg
    10921 tcctgttcga gaatgacgaa ctcgacgcgg cggttcttct ctttgccttc gggcgtgttg
    10981 ttgtccgcaa ttgggcgctt ggcgccgaag cccttggcgg tgaggcggcc tgcgtcgacg
    11041 cctttgtcga cgatgtactt catgaccgcc ttggcgcgat catcggagag cttcaagttg
    11101 tgagccgcat cgccctcgga gctcgcgtgc ccttcaatcg cgatcttctt gatgtgagcg
    11161 ttcttcttga tggtatccgc gatctcatcc atcaagctga acgaggcctg cagaatcgtg
    11221 gccttgttgt actcgaattg aatcttctcg ttgatttcga tcttgttgtc gcgcacctcg
    11281 acgcgcgccg gaggcggcgg cggctcctcc ttcttgggcg gcggaggtgg gggaggcggc
    11341 ggggccggcg gcgtgccgac cacactaatc gcgctctgtc cttggaacgt ggagacaccg
    11401 ccacaagcgg cggcgagcac cgtgatcgag accagcgtac tgtgaagaac tagcttcgaa
    11461 agaactagct gcgagcccgt gcgaaacgta tgcatcggtc gactcctggc taagcaccag
    11521 attggttacg ggggatcccc tgtaggaggg tactagtcgc atctcgggtt gtgtggagtc
    11581 aagccgccga ctacggacga tcccgtccac tcggccgaaa gcccgcgttt acagcacaaa
    11641 aacgcgaagc gcccccaagc tgggcctggg ggcgctggct ccacttcgat aagcggttac
    11701 gtgcgaatca cccgatctac ttggtacgtt acgggcggtc agcgacgacg ccgcttcagc
    11761 tctcgatgaa gctcttgagc tgcttcgagc ggctcgggtg acgcagctta cgcagggctt
    11821 tggcctcgat ctggcggatg cgctcgcgcg tcacctcgaa gtcctgaccc acctcttcga
    11881 gcgtgtgatc actcttctct ccgatgccga agcgcatccg gagcaccttc tcttcgcgtg
    11941 gcgtcagcgt cttcaatacg cggcgcgtct gctcgctcaa gttcatgttg atcacggcgt
    12001 cggccgggct caccacgctc ttgtcctcga tgaagtcgcc gagatggctg tcttcctctt
    12061 caccgatcgg agtctctagg ctgattggct ccttcgcgat cttgaggacc ttgcgcacct
    12121 tatcgagcgg caactccatc ttctccgcga tttcctcggg agttggctca cgcccatact
    12181 cctggacgag gtaacgtgaa gtgcgaatca gcttgttgat cgtctcgatc atgtgcacgg
    12241 gaatacggat cgtgcgcgcc tgatcggcga tcgcgcgggt gatcgcttgt cggatccacc
    12301 acgtcgcgta ggtgctgaat ttgtagccgc gcttgtactc gaatttgtcg accgccttca
    12361 tcaaaccgat gttgccctcc tggatcaaat ccaggaattg gaggccacga ttcgtgtact
    12421 tcttggcgat cgagaccacc aaccgcagat tggcttcgac caactcggct ttggctttct
    12481 cggccttgcg ctcacccatg cggatggctt gatacgtgcg gagcagctcg ccgacatcga
    12541 tcttgagctc ctcctcgacc ttcttcatgc ccttactcgc gttcttcagg gcctcatcca
    12601 tgctgtccag gtcgccgcga tcgacgccga gtttgcgcgc gagctcgtct tcggcggccg
    12661 gatcgtcctt ggcttctcgc agcatcttct tgagttcgct cttgctggcg ccggtgatct
    12721 tctcgagctc gatcaccttg ccttgagagc gatacacgcg ctcgatcatg gccttgagtt
    12781 tggccacgat cttgtcgatc gtcttcttgt tgagacgcat ctcctgcagc gtcttcacga
    12841 gctctttctt gttgtcggag agctctttgt cgatcagctt gcggcgcgct tcggcggcgg
    12901 tcttgcgctc ctcgagtagc tcggagttct tcttgtcgag tcggcgaact cgatcaatca
    12961 ggcgaatgat gcgacgatcc gcctcttctt cgtcgaactc gtgctcttcg tcttccgcgt
    13021 cgcgtacgat gtccttgact cgaatcttgt gattgcgcag gcgctcaccg atatcgatga
    13081 tctcgcgcac cgcgatcgga ctcgacagga tcgaggccaa gacctcgttt tcgccctctt
    13141 cgatgcgctt cgcgatctcg acttcgccct cgcgcgtgag cagcgcgaca ctgcccatct
    13201 tgcgcaagta catgcgtacg gggtcgttgc tcttgtagag ctcgtcgtcg ctcgtacgcc
    13261 cgggggtgga ggtggggccc tcggtggtcg tctcttcgtc ggccttcggc gcggccttgc
    13321 tcggcgcctc gtcttccgac acgcggtgcg gcgcgatctt gatgttgctg gcgtcgtcga
    13381 cgacttcgat ctcctcgtcg tcgagggcgc tcaacacgtc gtccatctga tcggggccga
    13441 cgtcctcgcc cggcagcgcc tcgtgcacgt cgtcgtaggt caagaagcct ttgctcttgc
    13501 ccaggttgat cagctgctcc ttggtcttgc cgttgacctt ggcctgtgag gcaactgggc
    13561 tcgacggagc ttcgtcgtcg tcgaagtcag cgtcgtcgct ctcactcggc ggcgcactcg
    13621 caggagcgct ggctttgctc gctttcgtgg aaagcttggc tttcggcttt tcgggcgccg
    13681 gtgctttctt ggcgacgctt gcagcggact tctttgccgg acgttctggt ttcttggatc
    13741 ccaccttgac ctcgctactc cgataccact gctctgtgct gtcgagccct gctgaagagt
    13801 tgcttgagca gctcctgctc ttgttcgaaa tctccagtgc ggcgagcgcg ctcgagttct
    13861 tcgaccacgt ttctcttgtc gcgccgcagg accaatgcct tcaactgcct gatgttccca
    13921 gagagctccg actgcgcgtc ctcggtcctc gaatgtcgcg gggccacgag acgggcgcgg
    13981 gcgaattgat ggatcgaggg cgggagcttc gcaagcactt gttccgggtt actgatggca
    14041 cccgccttcg aaagctgacg caatgcagca aaggcaattg ccgtatcccc ttcgagcagt
    14101 tcgcctgccg aactcgcttc ggggtcttcg aaaagctggg gaaagtccaa aaaaacgccg
    14161 agaatttcga gcccgatact gtctcttcga tccggcgatc gtgcgcggac gggcggagcc
    14221 acgagctctt tcttcggcgc cgaaacgcct gccgcgtccc gatccaacgc tcgtcgcacc
    14281 tcgtttacga gggcacgaaa cgtcggctcg tcggtaatat tcagacgctg ggcgatgcga
    14341 tccgcgtgcc gctccgcgag cgcccgcacg gtgggatcgg tttcctctcc cagcaattcg
    14401 atgacttttt tgaccctcgc cgcgcgcgtt cgcgcatcgc tctccgaaaa cgtttcgtct
    14461 agtacgccgt cgatcaagta ttcgagcaaa ccgcgcgcgc ggttcaggat tagatcgagc
    14521 gcctcgcggc cctgctttcg aatcagctcg tccgggtccg agcccgtggg caaagtagcc
    14581 actttcgcgc cgagcgctgc tttttggcaa acttcgcgcg cgccttgcac tgcccgcttg
    14641 ccggcctcgt cgccgtcgaa caacaagacc acgtcgttcg tgaagcgacg cagctgttga
    14701 gcctgctcca gcgtgaacgc ggtgccgagc ggagccaccg cgttcttgac gccgaacgcg
    14761 tgcaagctga ggacgtcgaa attgccctcc acgattacgg cgctctcctg ggtacgaatc
    14821 gcatttctcg cttggtatag accgaacacc gcctcgcgct tcttgtagat ggccgattct
    14881 ggcgagttgt aatacttagc cggcttttcc gcgcccgggg gtgagggtgc caatgaaagc
    14941 tctcttagct cggcggcggg cggctcttcc agagcccgac cgctgaaggc aatcggtcgc
    15001 ccttgcaagt ccaacaccgc gaacatcagg cgatgtcgga atcggtcgta gtaacctacg
    15061 gcgttcttac gcgccgcaac caaacccact ttttccgccg cgaccaggct cgccccgctg
    15121 ttcttcaagc tctgcgccag cccgtcccag ccgaacggcg cgtatccgac ccggaatgcc
    15181 tgcagagcgt ccgccacggg atcggtcggt gaactcggca cgagcttacg tcgctcgagc
    15241 tcggcgcggg cgtacgccga gagcggatgc tcgcgcaaca tgcgttcgaa gtatgcggcg
    15301 gccaagttca cgacttcgta cagctcgtcc gcgcgccgcc gcacttcgcg ctggcgcgcc
    15361 aactctgcgt cgctgatttg ccagtcgatg acgatcccgg cgcgatctgc caaacgccgc
    15421 acggcctctt ggaaatccag cccctcgatc tcctgcacga acttgaaagc gtcacccgac
    15481 gcatggcagc cgaagcagtg atagaagccg cgttccgcat tgacgtggaa gctcggggat
    15541 ttctctttat gaaatgggca caagccgacg aagcttctgc cgcgccgttg tagcttcagc
    15601 gattcgccga tgagcgcgac gagatccgtt tcgcgccgga tccggtcgag cgtgtcttgc
    15661 ccgatcacgg cagacgcata ccgcaaaatc acgccgcgcg ccgctccgcg aagtttttcc
    15721 tcggacctcg catttctcct caccggctgc tcgcgtcctc gtcatgggcc ctgcgtgtcc
    15781 ccccccggca ctgccacctc gcggcctctc ccagttaact gtccccaata ccgctcggca
    15841 gcagctggag gtgggtgctc ccgtttgctc gaggttcgtt cctcggcctc gcgctgaggc
    15901 ttgctcggag gctcgaatcg ttgcgcgcga ccgacacctt gttgttgggc ctcacggacg
    15961 cgcgggaaat ctgcaatcac tcgagtcccg gcgctgtgtc attaccaagc ccggtccgtc
    16021 gcgcaaaacg cagcagacta aacgatcagc gttggatgag tgtttctcgc ctaggggatg
    16081 tcagagaggc acgcacaaat aacgtactct cgagctggcg cactattcgg gcgttcagta
    16141 tcatggctct agatgctgcg tcgcttaccc gtcgcaaacc cgcagaaccg ctgggaacga
    16201 gcccatgtct cttacgagag cggagatatc ccgaatcagg ggctccagct gttcttggac
    16261 gactctcgcc gggtgatctc ggaaaatgac agccctgatg tttccttccg attcagtctg
    16321 aatccgtatc ggggctgcgc ccacggctgt gcgtattgct atgcccgacc gtcacacgag
    16381 tatttgggat tcggcagcgg aaccgatttc gaacggcgtt tgttgattaa accaagggcc
    16441 gccgagttgc tgcgcgaggc attcgaggcg aaagggtggc tgggcgatct actcgtgctg
    16501 agcggaaata cggactgcta ccagcctatc gaagcgcagc tcgggttgac gcgcggctgc
    16561 ttggaagtgt gcgtggaata tcggaatccc gtgcacatca ttacgaaggg cagcttggtc
    16621 gagagagact tggagctgct ggtgcgtctg cacgaagtcg catccgtgag tgtttcggtc
    16681 tcgattcctt tttgggatcc agacgcggcc cggtccatgg agccttatgc tcctccacca
    16741 gcgcggcgaa tcgagacaat ccgccgcctg agctctgccg gcatccccgt cgtggtgaac
    16801 gtcgcacctt tgatacccgg gttggtggat gcagacatga tccctatcct ggaggccgcc
    16861 cgcgcggccg gcgctctctc ggccatgagc attcctgtac gcctgccggg gagcgtggct
    16921 caggtcttcc aggagcgatt gcacgctcat ttcccgggca aagccgagaa ggtgctggcg
    16981 aggatccgcg aaatgcgtgg gggagctctg aatgagcccc gctttcacga acgaatgcgg
    17041 gccaaagggg aatatggcgc caccttattc agggtatttc aggcgacttg cacgcgcctc
    17101 ggcttcggag agttcccgga accgcgcata ggcacgttcc ggaggcccaa agctgcggag
    17161 aacggtgaca atagagctcg ggcacgtcgc cagacggcac gcgtcaaccc tcctctgggc
    17221 agcactcagc tgagtttgga tttcagcgcg acagcgcgaa acacctctga tgagggcaac
    17281 gcgccttagg aagagccgac gccaggagaa tcgcgccact ccgcgtgcga gagccgggca
    17341 gcacagagca caccgtcgcg cttggcagat ctttcctgcc atggtagcga agcacccgtc
    17401 ttagccatgg ctgaacaact ttccctcgag atcgtcaccc cggacggcgt gaaactgagt
    17461 gagcaggttt cggagctcac cgcgcccagt gtgaacggtg aattcggtgt cctacccggg
    17521 catcgtccga tggtggcggc cctcacgacc gggatcgtga gttatgtcca cggcggtgaa
    17581 aagcacgcgg tggccgtcgg tgcgggcttc gtcgaagtgg cggatgataa agcgttgctg
    17641 cttacggaca gattcgttcg caaagccgac gtggatccgg tcctggttcg caaagagctg
    17701 aaggaagtgg acgaggcgct cgacaagcta gagtccgacc agtcgagctc ggagtacggg
    17761 agcctggttg cccggcagct gtgggccgcg acccaactgg atctgtatgg agatccgccg
    17821 ccacctcggc ttcgcaccat gaacgaactc atgttcgctc agcacgaaac ctatgctgat
    17881 ctgacctcgg atgttgcctc gggagaagag ggcaccgaga agcacgccga cagcgaaaca
    17941 cactgaatcc agagctaacg ctgcgtggag ccgccgcaga gccccaaagc cgtgctcgcg
    18001 cagcgcggtt tgtcgccgaa gcatcacttc ggacagaact ttctcgtcga tcggtccatc
    18061 atcgagcgga ttgcggcatt ggccacgccg cgtccaggta cggtaatcga gttgggggca
    18121 gggctcggtg ccttgacgcg cgccttgctc gagcgagcca acttggtgat cgcgatcgag
    18181 cgcgatcgag acttgatccc gctgctcgaa caagacttca gcatcgattt aggtgccggg
    18241 cgcctgcgtc tactcgagtg tgacgccaag agcatcgagc cagccgcatt gtttgccgag
    18301 gggcagccgc cctacacgct ttgcggaaac ctgccttacc aaatcaccgg cccattgatg
    18361 cgcttggcga tggcgcacgc gcgtagcttc gaacgtgcag tgttcttggt gcagctcgaa
    18421 gttgcgcaaa ggctggcggc gggcccgggc tctgaagact acggggcttt gagcgtcttt
    18481 gcccaagcat cgttcaacgt aaaaagagaa ttcgtcgttc ggcgcggagc tttctttccg
    18541 cagccgaggg tggactctca agtagtctcc ttacagccgc gcaccgaccc aatcgcggag
    18601 acctcgctct tctcgaactt agtctcggct gctttcgaga agcgccgaaa acagctcggc
    18661 aacgcctggc gcggtctgcc gggggtggat cactctcgta tcgtcaaagc cgcagagctt
    18721 gccggaatcg atctgcaagc acgtggtgaa acgcttagtg gttcggactt tcatcgcatg
    18781 gagcgcgcga tcgaggagct agcgaggtga gaaacggctc cggagccttg ctcctgtcgt
    18841 gtgtcttgct ggcgagtagc tgtcggcgtg gaacggatcg aaataccgaa ccccgcatcg
    18901 agttcggggt tctgtttggc ggggacatcc aagatcggcc cgtgattcca ctggagctcg
    18961 atgcttcgcg ccaagaactc gcgctgcgag tcacttttcc agaacccgtt caacgcgaac
    19021 gtcgagttcg cttcgagctc gaacgccctc ccgcccccaa ccgagagaag ggc
//
LOCUS       DASTKU010001084         8528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_436218, whole genome shotgun sequence.
ACCESSION   DASTKU010001084 DASTKU010000000
VERSION     DASTKU010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436218"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..1598)
                     /locus_tag="VFQ61_38705"
     CDS             complement(249..1598)
                     /locus_tag="VFQ61_38705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960484.1"
                     /translation="MKLRPFSYSLVRFGLAPATLTGAVLLSPVLAGCSKKQPQITAVP
                     SASASAPSATSVAPPSADPELWSALTNITTACKVDEKQATVTCPQGEHRKLISEFVSN
                     RRSREKAVPTFAAALTDKNPALRAAASNVLYTGFRTNWGAQVQPGAVPAKDATELLNA
                     ALAAEKPLLRQALPAAVHACMIANQSEALYAAVEKAKEPQVRSVATRYYMTHGRLQAF
                     EKVRELAKDPDPSVSLAALEAPQNMTNWTPAEQAAICPWATELLNSDRPPVATKAAAL
                     LSNCQGEFVDKLQSLAEKALAAGKFTGSTLAGLRDLCSPSRRSSPNGPSEAQCKRSRK
                     LLEKVTEAKNVEEQTRSMALVAVAYQWPDDETLKLAQRLAKSKDAGLADHARRTIQRI
                     EQRKQSASKAAASGAPGSKPPSSRTAPDSRPALPSNPAQPTTPAPAANPGAEPTPGE"
     gene            1838..2470
                     /locus_tag="VFQ61_38710"
     CDS             1838..2470
                     /locus_tag="VFQ61_38710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960485.1"
                     /translation="MNRFSMLALAALPCLLACSGADQEDEKAPAPRASTGGSSLYIAM
                     GGSTAATGGASAGGTRGMQTLTSSGGVTATAGANPVAGTAGASGASTSPLPTPQGSGG
                     ATGLVWSNSGGVPNLPPDGSAGFGQAGAFPTPDPDQPFIDLGGDGWIQFGPGGGAPST
                     IPGFPAGGTTGFTIAGATGVPTTAGASSGGATTVIRFGSGNTAGTSQNTF"
     gene            complement(2674..4137)
                     /locus_tag="VFQ61_38715"
     CDS             complement(2674..4137)
                     /locus_tag="VFQ61_38715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET9960486.1"
                     /translation="MKKLLLAPSFAFLAFGFLAVCSCSDSNNTPGTSNGGSPSAGSSG
                     TGTGGGTGEATGGASATGGAVSTGGAASGGAVSTGGAATGGGSSTAGTAGTPSGENWV
                     GTWATGLQLTEPRNLPMGGDDTMPTPDNPGFVGNTLRQIVHVSIGGSKLRLRLSNEYG
                     VAPVTFDAVHVAKSMGSGAIDASTDTALAFSGKPSVTIPAGQAVNSDSFEFELAAMSD
                     LAISVKISEQSKDVTGHPGARTNSYMQPGDAVSLATLATDALKTAHWYFIAGVDVVGT
                     EKSGAVVVLGDSLTDGRGSTTDGNDRWPDALSRKLQSDAATSHIAVLNQGIGGNTVLT
                     GGLGPTAKVRFDHDVIAMPGAKWLIVLEGVNDIGNSTTDVSGDLIAAYEEFIEKAHKA
                     NIKAFGVPILPFKTNTNYDKGDHLDQRKKVNDWIRTSGKFDAVIDLDKVVADPADPDK
                     LLEAYATLPSSVGTDFLHLNPMGYKAMGDAVDVSLFK"
     gene            complement(5040..5300)
                     /locus_tag="VFQ61_38720"
     CDS             complement(5040..5300)
                     /locus_tag="VFQ61_38720"
                     /inference="COORDINATES: protein motif:HMM:NF025143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HET9960487.1"
                     /translation="MGNVVNLNKFRKQKAKAQREKQAEVNRRLHGRTKAERLRDDLQK
                     KQLTRGVEGARLSSETGATDGSPTDGAPTDGSPTEEAEPTKE"
     gene            complement(5408..5707)
                     /locus_tag="VFQ61_38725"
     CDS             complement(5408..5707)
                     /locus_tag="VFQ61_38725"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HET9960488.1"
                     /translation="MPTLTVLPSGKTAQVEAGITLLAAVNAAGEPLRTKCTDENCSGE
                     CHVFVPQGKKSLSKATREENAKLDTIVGVGSKSRLACQTRVGTEDITVELLGFAS"
     gene            6113..7027
                     /locus_tag="VFQ61_38730"
     CDS             6113..7027
                     /locus_tag="VFQ61_38730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960489.1"
                     /translation="MVVSLFSALMFTGSDARSFLQGQFTNDVRHLARERALMGSINSA
                     QGRAQSMLTLIERSEGIYAILPVDDLEPIQKRLRGFVLSAKVDVQVAQGWRVSPCSEH
                     SIANLPEHRGSKLEPGACLSGVDFTLLRWWSGDERYLLLARDTWSGPPIDAGCAETWR
                     LRDIQAGLPVIRPETRNSFVPQTINLDLLQGMSYDKGCYVGQEVVARQRRAGVRRRMG
                     RFASTCAPPTPGTRVFADSDGVSERIEVGEVVDAAATPQGCELLAVVDVQPPASPEDG
                     ASHGARGPLFLPNMIPLSPLTLPYLPSH"
     gene            7397..7720
                     /locus_tag="VFQ61_38735"
     CDS             7397..7720
                     /locus_tag="VFQ61_38735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960490.1"
                     /translation="MWIDARQTTDDSFRLVYRVHDSEHALFADTLEYRRVRGSWNPTR
                     TGPSESYPALARWIASRLFALRPRAAELRIRMERLVLHEDGSGFDGTGEFFYEVRVTR
                     SELSP"
     gene            7731..>8528
                     /locus_tag="VFQ61_38740"
     CDS             7731..>8528
                     /locus_tag="VFQ61_38740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960491.1"
                     /translation="MKRLLASWIEFWDRKEAPDSLVLSRILVGAVILVDLLAARWVGV
                     VPDLWAPPPEGLGIGMLQSPAPWTVQWFGASTQTALFMWWLGVVMAACFMLGAALLPA
                     GVLLAFTLAELARFTPSGDRGIDTLLRTAALILSLSGANARWSLDAWIRARFGRAPRE
                     RVPAWPRYLLFLQLIWLYSSAAHNRGDISWWPQGGFTALGLVLSDPHYARFAPGWAGN
                     IDGLLRAATAITMLFEGGSPLLIFLTWCDRGEGRGGRVGSWIRKWRVR"
BASE COUNT         1460 a         2601 c         2713 g         1754 t
ORIGIN      
        1 ttagcttcaa gtcgttgctt cgttccacgc atcgttcgac gaggcaaccc ctccaaaaat
       61 tgaactcaag tcacatgcgg tgcactagtg cgcgctgctg atgcgcgact ttgccagaat
      121 tacccccagg agttcgaaca agattgtttt agcaaccttg ttcgaaaaac aacctctctt
      181 acccaactcg aatagaccgc caagggttca gcgagaccat tcagctgggt tgccaatccg
      241 ctacgtagct attcgcccgg cgtgggttca gcccctgggt ttgctgccgg cgcgggagtg
      301 gtcggctgcg cgggattaga tggcagtgcc gggcgcgaat ccggggcagt cctggaagac
      361 ggcggcttgg accccggtgc tccgctcgcc gctgctttcg atgcggactg tttgcgttgc
      421 tcgattcttt ggatggtgcg gcgcgcgtga tccgcgaggc cggcgtcttt gctcttggcc
      481 agccgttgcg caagcttcaa cgtctcgtcg tccggccact gataggccac cgcaacgagc
      541 gccatgctgc gagtttgctc ttccacgttc ttggcttctg tcaccttctc gagcagcttg
      601 cgactgcgtt tacactgagc ttcactcggg ccgttggggc tgctgcgccg cgatggcgag
      661 cacaggtctc gcaggcccgc caaagtagat cccgtgaact tcccagccgc gagcgccttc
      721 tccgccaagc tttggagctt gtcgacgaac tcgccctggc agttgctgag aagggccgcc
      781 gctttggtcg cgaccggggg ccgatcactg ttgagcagct cggtagccca cgggcaaatc
      841 gcggcctgct ccgcaggtgt ccagttggtc atgttctgcg gagcttccaa ggccgcaagt
      901 gagacggaag ggtcaggatc tttggctagt tctcggactt tttcgaaggc ttgcagtcgg
      961 ccatgggtca tgtagtagcg ggtcgccacg gaccgaacct gaggttcttt tgccttctcc
     1021 accgcggcgt acagggcttc actttgattg gcgatcatgc aggcgtgtac ggcggcaggg
     1081 agagcctgcc gaagcagcgg tttttccgca gcaagcgccg cattcaagag ctccgtggcg
     1141 tctttcgccg gaacggcccc gggctgtact tgagctcccc agttggtacg aaatcccgtg
     1201 tacaaaacgt tgctggcagc ggcgcgcaac gcaggattct tgtcggtcag cgcggccgca
     1261 aaagtcggga ccgccttttc gcgggaacgc cgattggaga cgaactcgct gataagcttg
     1321 cggtgttccc cttgaggaca cgtcaccgtc gcttgcttct cgtccacctt gcaggcggtg
     1381 gtgatattcg tgagcgcgct ccagagctcc ggatccgctg aagggggtgc gaccgaggtt
     1441 gcgctcgggg cagatgcgga ggcagacgga accgccgtga tttgaggttg cttcttgctg
     1501 caccccgcca gcaccggcga gagcagcaca gcccccgtga gggtggcggg agcgagacca
     1561 aagcgaacaa gcgagtagga aaaggggcgg agcttcatct ttttgctcga aattgcgtcg
     1621 taccgcgaga ttcagtggtg tcttccgggc gaagttccaa ttttcagcag ggtagctgga
     1681 tggccggagc ggggcgactg ctttttctga gccgctgagg cgagtccctg gcgctctgcg
     1741 gcgctcgtgc cgcgaggacc ctgcggcgca cgttcggcca ggcacccgct taacccgttt
     1801 tggggcggtc taacacagta gcagtcgagg tacacagatg aaccgattct cgatgcttgc
     1861 tctcgccgca ttgccctgtt tgctcgcttg ttcgggcgcg gaccaagagg acgaaaaggc
     1921 ccctgctccg cgcgcttcca ccggaggctc ttcgctgtat atcgcgatgg gcggcagcac
     1981 cgctgctacg ggtggggcgt cggcgggtgg cacgcgcggc atgcagactc ttactagcag
     2041 cgggggcgtc acggccacag cgggcgcgaa cccagtcgca ggaacggcgg gcgccagcgg
     2101 cgcttcgact tctcccttgc ccactcccca aggttcggga ggggccacgg gcttggtttg
     2161 gtcgaatagc ggtggcgtgc ccaatttgcc tcccgacggc tcagccggat tcgggcaagc
     2221 gggagcattt cctacgccgg atccggatca gccgttcatc gatctgggcg gtgacgggtg
     2281 gattcagttc ggccccggcg gaggcgcgcc gagcacgatc ccaggattcc ccgccggcgg
     2341 caccacaggt ttcacgattg cgggtgcgac tggagtgccc acgaccgcgg gtgcgagcag
     2401 cggtggcgcg accacggtca tcaggtttgg cagcggcaac accgctggta ccagccagaa
     2461 caccttttga gtcgtccgcg catgggttcg aggctttcgc tctcgctccc tttcccctta
     2521 cagcgtcccc tctcgcggct tccctctaat cgctctcggc ctctttgcgc ctacgaatgc
     2581 agcgcgctcg caccgcagag cggcgctcgc aacggcagag cggcgctcgc aacggcagag
     2641 cgccgttgcg aacgcgaact cactatctaa ctgtcacttg aagagagata cgtccacggc
     2701 atcacccatg gccttgtagc ccatgggatt cagatgcagg aagtccgttc ccacgctgga
     2761 gggcagcgtt gcataggcct cgagtagctt gtctggatcg gcaggatccg caaccacctt
     2821 gtcgagatcg atcacggcgt cgaacttacc gctggtgcgg atccaatcat tgaccttctt
     2881 gcgctgatcg aggtggtcgc ccttgtcgta gttggtattc gtcttgaaag gcaggattgg
     2941 cacgccgaac gctttgatat tcgccttgtg tgccttctcg atgaactcct cgtacgccgc
     3001 gatcagatcg ccagaaacgt ccgtcgtgct gttgccgatg tcattcacgc cctcgagcac
     3061 gatcagccac ttggctccgg gcatggcgat cacgtcgtga tcgaaacgaa ccttggcagt
     3121 tggacccagc ccgcctgtca acacggtgtt gccgccaatt ccttggttca gcaccgcgat
     3181 gtgagaggtt gccgcgtccg attggagttt gcgcgagagc gcgtcgggcc aacgatcatt
     3241 gccgtcagtc gtgctgccac gaccgtccgt gagtgaatct ccgagcacca cgaccgcgcc
     3301 gctcttttcc gtccccacca cgtccacgcc agcgatgaag taccagtgcg cggttttgag
     3361 cgcgtctgtc gccagagtag cgagcgagac ggcatccccc ggttgcatat acgaattcgt
     3421 acgcgcgccg ggatggccgg tcacgtcctt ggactgttca ctgatcttga cggaaatcgc
     3481 cagatcggac attgccgcga gctcgaactc aaagctatcc gagttcaccg cttgacccgc
     3541 cggaatggtg accgagggtt tgccggagaa tgcgagagcc gtatcggtgc tcgcgtcgat
     3601 tgcaccagag cccatggact tcgccacgtg cacggcatcg aaggtgaccg gtgcgacgcc
     3661 gtactcgttg gagagacgca gccgcaattt gctccccccg atcgaaacat gcacgatttg
     3721 acgaagcgta ttgcccacga atccaggatt gtcgggggtg ggcatggtgt catcgccgcc
     3781 catgggcaga ttccgaggct cggtcaattg aagcccggtt gcccaggtgc caacccagtt
     3841 ctctcccgaa ggagtacccg cggtgcccgc ggtggaggag ccaccgccgg ttgcggcgcc
     3901 gccggtcgag accgcgccgc cgcttgccgc cccgccagtc gagaccgcgc caccggttgc
     3961 cgacgcgccg cccgtggctt cgcccgtgcc gccaccggta ccggtaccgc tggagccagc
     4021 cgacggggaa ccgccgttcg acgttcccgg agtgttgttg gagtcagaac aagagcagac
     4081 cgcaagaaac ccgaaggcca agaaggcgaa cgatggagca agtagcagct ttttcattgg
     4141 ttcttccaag gcgaactgga cgcgagcacc gtgcgttcgg agccgagcgt gagacaggta
     4201 aaggcaaccg ccgctttgtg tccacgagca cacgatagcg gctcagtgcg gccaattaga
     4261 gcgcctccat ccagcagcgc ctcgatccag cgcggatcct tcaagccgag cgcttgctag
     4321 cggcttttgc aatgcgccga ggccgcgcga ttagagcacg aacaatggcc cacgagccgg
     4381 gttcatttca gcaggtgggg caacgcaccg cgggctgtgt cgaggtgcag attccttatt
     4441 gaactgcttt ctgccaaact gccgcacccg tgcccctccc ggaggcctcc ttctctgccg
     4501 gctgctcgcg gctcgcatcg gctcacgtgc tgcggagggg ctaccgtgca ggtagttggg
     4561 cataccgagt gcgaacccga gagttagccc tcgtcggatt ccgagcggga ggtgacttgc
     4621 accacgaagt cgccgctaaa actcgcactc gccccactcg cttcgaatcc gcgaaagact
     4681 cagcaaagct gcgtgtatca gctcagcgtg gttcgaaata ccattgcggt tcgaggcgcc
     4741 aattaggcaa gagcagcctc gcgaatcttg acgatgccgc acgcgccgtg agatctggat
     4801 tcagtccgct cgctccgccg cagcacttcc gccagcccct cccgagggcc ctttgcccgc
     4861 gctcgcttca agccatcaat tcccaagctc ggcgcgacct gaagccaagc gtctcgacac
     4921 cactcgacag tcgataggga atcggagtcg actgaggatt gcactacgct tcacgcggcg
     4981 agagtagcgc aggcacccgc gctcgtgtcg gacgttacat cccggaggac gcgcgggcct
     5041 cactccttag tgggctcggc ttcttctgtt ggtgacccgt ctgttggtgc cccgtctgtt
     5101 ggtgacccgt ctgttgctcc cgtctccgag gagagtcggg ccccctctac cccacgcgtc
     5161 agctgcttct tttgcaggtc gtcgcgaagc ctctcggcct tagtgcgccc gtgcagtcgg
     5221 cgattgacct ctgcttgttt ttcgcgctgc gccttggcct tttgtttgcg gaacttgttc
     5281 agattgacga cgttgcccat agcggcttcc gtttttacgc gcgggagcca tcgaagcacg
     5341 acgagcccag gcgcacaacg gactatgcgc ctggcctcag tcaaatgggg tcactaggat
     5401 ggcgtgacta ggacgcaaaa cccaggagct caacggtaat atcttcagtg ccgactcgag
     5461 tttggcaggc caggcgagac ttggagccta cccccacgat cgtatcgagc ttggcgttct
     5521 cttcccgcgt cgccttcgag aggctcttct ttccttgagg cacgaagaca tgacattcgc
     5581 cactgcagtt ctcgtccgtg cacttcgtgc gcagcggctc tcctgcggcg tttactgcag
     5641 ccaatagcgt gattccagct tctacctgcg ccgtttttcc gcttggtaac acagtcaggg
     5701 tgggcatgct caaataccat tcctcgagat tcggttcccc gccgcgcgct cgatccgctc
     5761 gagaccgaag ttcgcggtgg tcacggtcgc gtcgtgtttg tgtcgcaccg tgttctttgg
     5821 ggcgtgtttc ggggcgtgtt ttgggcgcag tgcaaaggtc gtgcgagggg cggtgcaaga
     5881 ttcgaatgcc tcgggcgagc ggcaatttgt gggatttcga gttagcagaa cgtcgccggg
     5941 ctggtcgcga gcccgacaag cgacaggata caatgtcgca aaggcgacct ctctgcggct
     6001 ccgccgacaa gaagcggccc ccatcccacc gattagagtc gcagcaagcg ctggcatgtg
     6061 ccttgctgag tgcgagcctc agcgggctca gcgacgcgag gaatcaccgc ggatggtagt
     6121 ctccctattt agcgcgctca tgtttacggg cagtgatgcg cgcagctttc tgcaaggtca
     6181 gttcaccaat gacgtgcgcc atttggcccg cgagcgcgcc ctgatgggaa gcatcaactc
     6241 ggcgcagggg cgcgctcagt cgatgctgac gttgattgag cgaagcgaag gaatctacgc
     6301 gattctgccc gtagacgact tggagccaat ccagaagcga ctacggggct tcgtgctaag
     6361 cgcaaaagtc gacgtccagg tcgcgcaggg ctggcgagtc agcccttgtt cggagcactc
     6421 catcgcaaac cttccagagc accgcggttc gaagctcgag ccgggcgcat gcctgtccgg
     6481 cgtcgacttc acgcttctgc gctggtggag tggagacgaa cgctatttat tgcttgcgcg
     6541 tgacacttgg agtgggccgc caatcgacgc cggctgcgcc gagacctggc gcttgcgcga
     6601 catccaggcg ggactgcccg tcatccgtcc ggagacacgc aactctttcg ttccgcagac
     6661 aatcaatcta gatctgcttc agggcatgag ttacgacaag ggctgttacg tcggacaaga
     6721 agtagtagcg cggcagcgta gggctggcgt tcggcggcga atgggccgct tcgctagcac
     6781 ttgcgcgccc cctacgcccg gcacgcgggt gttcgcagat tccgacggtg tatccgaacg
     6841 gatcgaggtg ggcgaggtcg tggacgcggc cgcgacgccc cagggctgtg aacttctcgc
     6901 cgtcgttgac gtgcaacccc ctgcgtcgcc ggaagatggc gcttcgcacg gcgctcgcgg
     6961 ccctctgttc ctaccgaaca tgatcccgct cagcccgttg acgttgccgt acctgcctag
     7021 ccactgagcg gaatgagacg ttcgtgcggg cgcgccgatg ggtgggcgtg gctcgccgta
     7081 ccgccaccgg aagaactgcc cgcgttgatt ttccgggcac gggcagatac caatgctgcc
     7141 cttgcgagca gtcaacgagt actggccggc aagtcgagcg gctctcatca gcctggcgat
     7201 ctgtatcgga ctaatagacg gagcgccatt gccaggggaa gccaacgcgc gcgtaccggt
     7261 ggcttggacg gctgccctgc ggcgggttcg agagtggcga gcgcgaaccc tcgaatcagt
     7321 cggtatattg gggtcgacgt ttggactttc gcaacggtgg gggctattcg cgtcccccgg
     7381 acgcacacgt taccggatgt ggatcgatgc acggcaaact acggacgata gtttccgttt
     7441 ggtctatcga gtgcacgact cagagcacgc cctgttcgcc gatactctgg agtatcgaag
     7501 ggtacgcggc agttggaatc ctacgcgcac tggcccgagc gaaagttacc cggcgctcgc
     7561 ccgctggata gccagccgtc ttttcgcgct gcgtccacgc gccgctgagt tgcgaatccg
     7621 gatggagcgc ctcgttctgc acgaggatgg cagtgggttc gacggtacgg gcgagttctt
     7681 ctacgaggtg cgtgtaaccc gaagcgagct ttcgccgtga cttccgcagc atgaagcgac
     7741 ttttggcaag ctggattgaa ttctgggacc gcaaggaggc gcccgatagt ctagtgctat
     7801 cgcgcattct ggtgggagcc gtgatattgg tcgacttgtt ggcggcccgc tgggtcggtg
     7861 tggttcccga tctctgggcg ccccctcctg aggggctcgg gataggcatg ctgcaaagcc
     7921 ctgcgccttg gaccgtgcag tggtttggag cttcgacgca aaccgcgctg ttcatgtggt
     7981 ggctcggcgt ggtgatggcg gcgtgcttca tgctcggcgc cgcgcttcta cccgcggggg
     8041 tgctgctcgc gttcacgctc gccgagttgg ctcgcttcac tccgagcgga gaccgcggca
     8101 tcgatactct gttgcgcacg gccgcgctaa ttctctccct ttcgggggcg aacgcgcgtt
     8161 ggtcattgga tgcttggatc cgggcacgct tcgggcgcgc tccacgagag cgggtaccgg
     8221 cgtggcctcg ctatctcttg ttcctgcagt tgatttggct ctactcctcg gccgcccaca
     8281 atcgcgggga catttcttgg tggccgcagg gtgggtttac ggcactggga ttggtgctga
     8341 gtgatccgca ctacgcgcgg tttgcgcctg gctgggcagg aaatatagat gggctgctgc
     8401 gggcagcgac ggcgataacg atgttgttcg aagggggatc tccgctgcta atctttttga
     8461 cttggtgtga tcggggagag gggcgcggcg ggagagtggg tagctggatc agaaagtggc
     8521 gggtacgg
//
LOCUS       DASTKU010001085         7313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_214982, whole genome shotgun sequence.
ACCESSION   DASTKU010001085 DASTKU010000000
VERSION     DASTKU010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214982"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VFQ61_38745"
     CDS             complement(<1..925)
                     /locus_tag="VFQ61_38745"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HET9960492.1"
                     /translation="MAADEGSGGYVTTQSGPLASEGSPLSWFQERLWVHYRRSPENTS
                     YNLPLMLLVRGQLDVPALEQTLSEIVARHESLRTVYGQTEEGEPVQFVTPPERVRLPV
                     VTVDRAQLLELLDRHLEHRFDLRKGPIFIVSLLRLSTDRHLLLFNVHHIAADAWSLKA
                     IFLAELQSGYAAFCRAERPAAPPLNVQYKDYAARQRSSDMSADLEYWCQALEGYEDSL
                     ELPSEQTRQAKSGTTSASFVYRYPREFSQSLERFSRQHGCTIFMSLLAALGVTLSRYT
                     NKEDLCIGTTTSNRTDVEFEPLIGFFINILPL"
     gene            complement(1519..2685)
                     /locus_tag="VFQ61_38750"
     CDS             complement(1519..2685)
                     /locus_tag="VFQ61_38750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960493.1"
                     /translation="MKASLGGLSFEVWSSVPRAVCRRGGLGWAMLLGLALLGSAGLAC
                     SSSEKTPQNGLQPEPPSAPVAEADFCAELAAARCVGIQECCPVAGSKYADSSSCVEAE
                     KSRCAASPMALFASGSFTYDAEGGGALIARLKRAAQGCNEPDGLLNWASLAKPALGLG
                     ASCQIDLSLPAGDPCKDGFCSLTAGTTSGTCAALPIEGELCPSQICAAGLSCKNESGG
                     ARCRPLPGEDAKCSGSDSPCAAGLVCQLTQQSLDEFGTGEDAAWEFACRPPRKVGETA
                     IHAQACESGVGMIRRNTTLAPVCATCSRHEDCMYDRDAPSDFFSADPAFGYCDRGACK
                     EGQENVIPRSKPSGAVCFNANECRSLNCAGPDGMLKHCGEPNLEALFCKVSASP"
     gene            complement(2943..3398)
                     /locus_tag="VFQ61_38755"
     CDS             complement(2943..3398)
                     /locus_tag="VFQ61_38755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960494.1"
                     /translation="MTPEPQTIARRVLTLHLGIALATSAFICLFMMLLCLFQGDGGWS
                     WTFFSILCAVFFPVLVTRKLGETVRIHALALPDIAANVGVRRSLIRQLARWLGVVGLA
                     LTLTLMVPISYLPLHNLRFTLVTTGLHAALFVIMTIDQLLSVKKSLSIG"
     gene            complement(3473..5770)
                     /locus_tag="VFQ61_38760"
     CDS             complement(3473..5770)
                     /locus_tag="VFQ61_38760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960495.1"
                     /translation="MKRCRNDAECPASTAVCTTKGYCAECESQSDCPDVYHCTAGRCE
                     LDQCEPGEHRCQSDAKAVISCTTQGELDVKACRDNETCSGVGSESACVTQLCSPGAWS
                     CEGSQKAALCSKDGLSFEEVVNCSDSDRVCLEGQCRAPVCESKAWSCGKDSLYRCNAA
                     GTAEALVKTCASGTYCDSEAGDCITGACTPNALSCDGNWLRACNAEGTGYAEEGTDCS
                     ATGQLCVEGSCRASACDQEYQCQGSDSYRCVEGGSRLALDKKCDPLGAPAEYCNPESG
                     QCEPVVCAPGQPVCDEKLATVCLDDGSGPSDQGIDCAAQGLTCWEGECLLEICSQPFR
                     CDGEKLYECIHDGTELLLIEDCQSADLCDAGNGVCLAQLCTPGEPACSGATAGTCDET
                     GHALEPGGTDCSKANRACRMGECVERVCAPDAFVCVDGDVHRCDGSGGATELIDACST
                     GEHCLPGVQQCVPDRCAAGSPVCDGSRATTCLADGSGPSATGTDCSANGQLCYLGMCR
                     NLICEPSARYCKDGQVRQCSTTGLASSPEVNCLETEFCEASGFDARCVPDRCAANQQS
                     CDGEWLALCNADGSGVASKSADCAADGLLCTLDGQCAARAEDTVGNSTVSASNPFANL
                     YLTVVEATTSRRLSDLTTRLGFSAGTPLDFVIYEASSWPGTFTRKWLSSVSAVSTTPG
                     TPTHVTPDFVLLQGKIYALGVLVHAAHQSAEARDGSSFVSFGRVLGEVNAPLTTVPSE
                     PGATLDIMTLRPATTSLELVTLSVN"
     gene            7012..>7313
                     /locus_tag="VFQ61_38765"
     CDS             7012..>7313
                     /locus_tag="VFQ61_38765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9960496.1"
                     /translation="MISRRCVQRQFLLRPDDRTNQCFLFCLALAAQRAGVRIVAFVAM
                     SNHYHAIVHDPLGTLPVFMEHLHKLAARALNARWGRWENLWAAEQTCATHLVNPADV"
BASE COUNT         1447 a         2391 c         2166 g         1309 t
ORIGIN      
        1 ggagcggcag gatgttgatg aagaagccaa tcagcggctc gaactccaca tccgttcgat
       61 tggacgtggt ggtgccgatg cagaggtcct ccttgttcgt atagcgactg agcgtgacgc
      121 ccagcgccgc cagcaacgac atgaagatcg tgcagccgtg ctgacgagag aagcgctcta
      181 ggctctgcga gaactcacgc gggtagcgat atacgaagct cgcgctcgtg gtgccggact
      241 tggcttgccg cgtttgctcc gatgggagct cgagactgtc ttcgtatccc tcgagcgctt
      301 ggcaccaata ctcgaggtca gcggacatat ccgaagaccg ctgccgagcc gcgtagtcct
      361 tgtattgaac gttgagcggc ggagccgctg gccgttccgc acggcaaaaa gcggcgtaac
      421 cgctctgaag ctccgccagg aaaatagcct tgagcgacca agcatccgcc gcaatgtgat
      481 gcacattgaa aagaagcagg tggcggtcgg tcgataagcg cagcaggctg acgatgaaga
      541 ttggaccttt gcgcaggtcg aaacggtgct ccaggtgtcg atcgagcagt tccagcagct
      601 gagcgcgatc caccgtgacg acgggcagtc ttacgcgctc aggcggcgtc acgaactgca
      661 cgggctctcc ctcttcggtc tggccataaa cggtgcgcaa gctctcgtga cgcgcgacga
      721 tctcgcttag agtctgctcg agtgccggaa catccagctg gcctcggacg agcagcatga
      781 ggggcaggtt gtagctcgtg ttctcggggc tgcgtcgata gtggacccaa agtcgctctt
      841 ggaaccagga cagcgggctg ccctcgctcg ccaggggccc cgattgagtc gtaacgtaac
      901 cacccgaacc ctcatcagca gccatacctg ttcctccaag acgtagatcg gtcaagcgtg
      961 ccagcgattc gtaatccgct ggtaataggg gggctacatt ggcacgaaac tcaaaccagc
     1021 gtaaggctct tgaacgctcg gaaccccgcg atcgcattgg ggttcgtctg ctgttaccga
     1081 actggtgatg tctctgaaaa gatcagctca cgattccgcg agccatcgca ccgcacgagc
     1141 actcgcgtca agaacgtttc tgcgatgagg ctcgcagatt cggtcgagcc cggggtgttt
     1201 ccagtggttt cgactgcggc cgtcagcgac tcgaaccggc ggctacttca accactggat
     1261 atagattagg cgcccgtcca gatcgtccca cgctgcaaca gcgtacctgg gaccttgcgg
     1321 tttgaaacta gcctgcacag cctgcaggcc gttctgatat tggtcagtgt gcattgtgac
     1381 acaccgacgc cggctcgcga tgcggatgcc gttcacctga gcagcagcgg agttgctgga
     1441 tgttgacgac agtccgcagc gcgccgggct tacgtccggt taactggtct aagagctagt
     1501 ccaacagcca gcaagcgctc agggcgacgc ggaaacctta cagaacagcg cctcgaggtt
     1561 gggctccccg cagtgtttca gcattccgtc tggcccggcg cagttgagtg agcggcactc
     1621 attggcattg aagcacacgg ctccgctcgg cttgctgcgc ggaatcacat tttcctgacc
     1681 ctctttgcag gcaccgcgat cgcaatatcc aaaggcagga tccgccgaga agaagtcgct
     1741 gggcgcgtcg cgatcgtaca tacaatcttc gtgacggctg caagtcgcgc acaccggggc
     1801 gagcgtggta ttgcgccgaa tcatgccgac accgctctca cacgcttgcg cgtgaatcgc
     1861 tgtctcgccc accttacgcg gcgggcggca tgcgaactcc caagctgcgt cttcaccggt
     1921 cccgaactca tccaaagact gctgcgtcag ctgacacacg agccctgccg cacaaggaga
     1981 gtccgagccc gaacacttag cgtcttctcc gggcagcggc cggcaacgcg cgccgcccga
     2041 ctcattctta caactcaagc cagcggcaca gatctgactc ggacacaact cgccctcgat
     2101 cgggagagcc gcacaagtac cgctcgtcgt ccccgcggtc aacgaacaga agccgtcctt
     2161 gcaggggtcc cctgcgggaa gcgagagatc gatctgacaa ctcgcgccga gcccgagtgc
     2221 cggcttggcc aggcttgccc aattcaacag accatccggc tcattgcaac cctgtgcagc
     2281 gcgcttgagt cgcgcgatca gcgcccctcc gccctccgcg tcatatgtga atgagccgct
     2341 cgcgaacagc gccatcgggg atgctgcaca gcgactcttc tccgcctcca cgcagctact
     2401 cgaatcagcg tacttgctcc ccgccactgg acagcactct tgaatcccca cacagcgagc
     2461 cgccgcaagc tccgcacaaa aatcggcttc agccaccggg gcggacggcg gctccggctg
     2521 cagcccgttc tgcggcgtct tctcggacga cgaacaggcc aaccctgccg agcccaaaag
     2581 cgccaagccc aaaagcatcg cccaacccag ccccccacgc cgacataccg cgcgcggaac
     2641 cgacgaccaa acctcgaacg acaacccacc aagtgacgct ttcatgaata accccgcctt
     2701 ctatcacggc gctcgccttc gatgctaggc ctccgtgcga agccgtccac ggtgccgcga
     2761 tcgggagctt ggcgttgaca gcgccttcac gagcgtgcgt tatcgagggc gctaggcgcg
     2821 gtgtttcggg aacttgtaag gattgttgcc tgtgccatga cgaatggaga cttggggtcg
     2881 cgcccgtgat tgcgagttgc gatcggaggc ctaacgtcga acggacgggc gcttatgttc
     2941 aatcatccga tcgataggct ctttttgacg gacagcagtt gatcgatcgt cataatgacg
     3001 aacagcgcgg cgtgcaggcc cgtcgtcact aatgtgaatc gcagattgtg cagtggcaga
     3061 taggatatgg gcaccatcag cgtcagcgtc agcgcaagcc ccacgacccc aagccaccgc
     3121 gctaactgcc gaatcagcga tcgccgcacg cctacattcg ccgcaatatc gggcaaagcg
     3181 agcgcatgga ttcgcacggt ttcacccaac ttgcgtgtta ccagcacggg aaagaagacg
     3241 gcgcagagaa tcgagaagaa cgtccaagac cagcccccgt ccccctgaaa caggcatagc
     3301 agcatcatga agagacaaat gaacgcgctg gtcgcgagtg caatcccgag atgcagagtc
     3361 aagactcgcc gggcgatcgt ctgcggttca ggggtcatga tgaggacgtc cagtgtatgg
     3421 cgtcgcgtca ggttttgcac gcccgggttc actcctcggc ggccaattgc gtttagttta
     3481 cagagagtgt caccagctcg agtgacgtcg tggccgggcg aagggtcatg atgtcgagtg
     3541 tggccccggg ttcgctcggg acagtcgtga gcggggcatt cacttcgccg agtacacgcc
     3601 cgaagctgac gaaactgctg ccgtcacgcg cctcggcgct ttgatgcgcg gcgtgaacca
     3661 ggacccccaa cgcatagatt ttgccctgca gtagcacaaa atccggagtg acgtgggtgg
     3721 gggttcccgg agtggtgctc accgcggaaa ccgagctgag ccacttgcgc gtgaaggtgc
     3781 cgggccaact gctggcttca taaatcacga agtcgagcgg cgtgccggcg ctgaatccaa
     3841 ggcgagtcgt cagatcgctg aggcgccgtg aggtcgtggc ttcaactacc gtgagataga
     3901 gattggcaaa cggattggag gcactgaccg tgctgttacc cacggtgtcc tcggcgcgcg
     3961 ccgcacattg accgtccaat gtgcagagaa gcccatccgc tgcgcaatcc gcggatttcg
     4021 atgcaacgcc ggagccatct gcattgcaca gcgccagcca ctcgccgtcg caactttgct
     4081 gattcgcggc acagcgatcc ggaacgcacc gggcgtcgaa accagacgct tcacagaact
     4141 cggtttccaa gcagttgact tcggggctcg aggccagccc ggtcgtgctg cactggcgaa
     4201 cctgcccatc cttacagtaa cgagcgctgg gctcgcagat caggttgcga cacataccca
     4261 ggtagcaaag ttgtccgttt gccgagcaat ccgtgcccgt tgcgctcgga ccggagccat
     4321 ctgccaggca ggtagttgcg cggctgccgt cgcagacggg cgaaccggcg gcgcaacgat
     4381 cagggacaca ctgttgaact cccggaaggc agtgctctcc ggtagaacaa gcgtcgatca
     4441 actctgtcgc gccgcccgag ccgtcacagc gatgcacgtc gccgtccacg cacacgaaag
     4501 cgtccggtgc gcagacgcgc tcgacgcact cgcccatacg gcacgctcgg ttcgccttgc
     4561 tacaatcggt gcctcccggt tcgagcgcat gcccggtctc atcgcacgtg ccggccgtcg
     4621 cgcccgaaca ggcgggttcg cccggcgtgc acagctgagc gaggcatacg ccattgcccg
     4681 cgtcgcacaa atcagcactt tggcagtctt caatcagcag cagctccgtg ccgtcatgga
     4741 tgcactcgta aagcttctcc ccgtcacatc ggaatggctg actgcagatc tccagtaagc
     4801 attcgccctc ccagcaagtg agcccctgag ccgcgcaatc gatgccttgg tccgaagggc
     4861 cagacccatc atcgagacac accgtcgcga gcttctcgtc acacacgggc tgcccgggcg
     4921 cgcagactac gggttcacac tggccgctct cggggttgca gtactcagcg ggcgcgccga
     4981 gcggatcaca cttcttatcg agcgcgagtc gcgagccgcc ctccacgcat cgatacgagt
     5041 ctgagccttg gcactgatat tcctgatcgc acgcgctcgc gcggcacgag ccctccacgc
     5101 acagctgccc cgtcgcactg cagtccgtac cttcttccgc gtagcccgta ccctccgcgt
     5161 tacacgcgcg cagccaattg ccgtcacaag acagcgcatt cggcgtacaa gcgcccgtga
     5221 tgcagtcgcc cgcttcggag tcacagtagg tgcccgaggc gcaggttttc acgagcgctt
     5281 ccgccgtgcc cgccgcgttg catcgataca gcgaatcttt tccgcaggac caagccttgg
     5341 attcacacac cggcgcgcga cactgcccct cgaggcagac acgatccgaa tcggaacagt
     5401 tgaccacctc ctcgaaggaa agcccgtcct tcgagcataa ggcggctttc tgcgaccctt
     5461 cacagctcca agcgccgggt gagcacaatt gagtgacgca cgcgctctcg ctccccactc
     5521 ccgagcaagt ctcattgtct cggcaggcct ttacgtcgag ctcgccttgg gtcgtacaag
     5581 agatcaccgc tttggcatcg ctttgacaac ggtgttcacc cggctcgcat tgatcgagct
     5641 cacagcgccc agccgtgcag tgatatacat cggggcaatc gctctgtgat tcgcactctg
     5701 cgcagtaacc cttcgtcgtg caaaccgcgg tggaggcggg gcactccgcg tcattccggc
     5761 acctcttcac gcactcccgc tcgacgcact gctcgtcgct cgcgcgacaa tccgcgtctg
     5821 cgacgcactc tacacattcc cgtgaatccg gtgcgcagcg aaatcccccc gtacaatcga
     5881 tcgaactccg gcagctctgc gcggccacac actccgctgc cgagcaatga tagcccggcc
     5941 cacaatgctc atccgtctgg cacgccacac agaacccagc aactagatca cagaccggac
     6001 tcgagcctgc cttgcagtcc gcgttggacg agcagcgcac agcgctcacg caagctcgat
     6061 cttcgcaacg cataccgctg ccacaatcgc gatcgaagag acactgcacg catccccgga
     6121 gcgggtcaca gcgcttgggg atgcacacgg aatcggaggc gcagagcgcg acgcccgggc
     6181 caggtctcgg cgcgggatcc tctccgcagg cggcgagcac cagcggcgca gcgacgccca
     6241 acacaagcaa agcataaaca gagttcatag cgattgggcg tgagtgaccg aacgaggtta
     6301 atgaaagcgg ggccagcagt tggcgcgaac ctagcgcgtt gctttacgga cgccaatggc
     6361 atcaccacga cgctcatgag cgcgcaccgt cggttcaggc tcgcgtgatg ctccccctca
     6421 gcggagcacg ggagctcgcc ggtccgtgct cgctcccgct gcgcgcggcc cgaggccctc
     6481 aaggccatgc tcgcgcctcg gcggcgctgc gcgtggccga gggtcctgca tcgccagcga
     6541 ggtcgatgca acggtgcagc gccacgggtc gccactggcg gccactccgt ggatcactcg
     6601 tcggcgcgta tctcggattg cgcacgctcg atgcgctagg gtgtcaagcg atgccgtcgc
     6661 tcgatcacga gacgctcatc ctgctcttcc gcaaaccagc ccgagctcgc cgcgcagctg
     6721 ctccgcgaag cgcttgacct cgatgcgagc ccgaacgagg ttaaagcatc ttgcgtgttg
     6781 tgtagggatg ttgtgtgtca ctcgccatgc aagatcgcca gtgcagtatg aggccgatac
     6841 ccccgagcgt gagaggctgt cgcgtctaaa caaagcgttg ctgccagccg tcgccaccgg
     6901 cggccacccc ggcagtcgcg gcctacccgc gaattcaggt tggcacacag cgcgcttggg
     6961 tcaccgttcg cgatgtgtgc tcctcgccag atcatacccg gccgttattg gatgattagc
     7021 cgccgctgcg tgcagcggca gttcctgttg cgtcctgatg accgcacgaa tcagtgcttc
     7081 ctgttttgcc tggcgttggc ggcgcagaga gcgggagtcc ggatcgtcgc cttcgtggca
     7141 atgagcaacc attaccacgc gatcgtgcac gacccgttgg gcacgctgcc ggtgttcatg
     7201 gaacacctgc acaagcttgc ggcgcgagcg ttgaacgcgc gctggggccg ttgggaaaac
     7261 ctttgggccg cggagcagac gtgtgcgacg cacctggtga acccggccga cgt
//
LOCUS       DASTKU010001086        23048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_660852, whole genome shotgun sequence.
ACCESSION   DASTKU010001086 DASTKU010000000
VERSION     DASTKU010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 23048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23048
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660852"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VFQ61_38770"
     CDS             <1..268
                     /locus_tag="VFQ61_38770"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET9960497.1"
                     /translation="VFDRLAAHKTVATRLAGDKRFEFVFLPAYAPQLNPDEWVWRYAK
                     HTLLGGCCPWGYDDLQNAVHSALSSFKSHSDLLASFFRGARLSL"
     gene            1016..1486
                     /locus_tag="VFQ61_38775"
     CDS             1016..1486
                     /locus_tag="VFQ61_38775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960498.1"
                     /translation="MVNWLRPVRQLRPPPQHMPLEAIKMLTFPILMLFGEHVASFKDE
                     AALRRSLSLVTDKGLKNGFIGDTILVDSDLNQHAILAFQLKGREPLFGWLRSGREIIQ
                     FETTPARRTTLPDIKRLLLKRISQSEWEDVPSLVAAIESATEFSALVEPFAVSF"
     gene            complement(1780..2901)
                     /locus_tag="VFQ61_38780"
     CDS             complement(1780..2901)
                     /locus_tag="VFQ61_38780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960499.1"
                     /translation="MRVRTLPTLVAALAATGYLHTTAPAAACSAPPTRDTLFFEVPSS
                     VPVDGVVLIRTSCDTTCPTDPIFEVSDASGNVVTGNVVQTLLVQGDTWLVWKSQTNLA
                     EGESYSVALLDTEELEYEAAQHIFQAVTLPTTPSSSWHVTDIAARAVLEGETVCCKEG
                     ERDSCNELPCYLVPSSVHGVVNALLETPERADGSQYLYRVQPQDGERWMVGNHLFASF
                     ALAEKYCYEIEALDLATGAVQTVAQDCISGAEVTAPDLELALGNAQVDLFRQCTVPPA
                     GYEDVWCRTYAQPRRYHDCAEQYPLEACEAALNACPSLVGQAGSTGLNFDEVITSDER
                     DRGCTMGSSSSSGLTLPGMLAALALAGSRMRRRIRNRRG"
     gene            complement(3188..5185)
                     /locus_tag="VFQ61_38785"
     CDS             complement(3188..5185)
                     /locus_tag="VFQ61_38785"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HET9960500.1"
                     /translation="MSERISVTEVRNALRCPRIFALGRLYQQAVAFPVGSSCLGAAFH
                     RIVELFSRDVEHPPAHFASLQPGTPRDETEAHLAQWLLDYLFRELEADVAYATMPGEV
                     DDLAEALREFARHLAGRLQGFAVRPGAALPQVVRTGERAVEARVQEDGPVVHGRLDAL
                     YSDARGNLEVVEYKLTDEANDEMDRAQVALYRSLLRASDGGDATPVILRFTPTLRETA
                     LTAESADALVDRVVQPLLDQMASWLATPESAPPTHRKDLCSACPVARECAETYPERLA
                     VRDDPPAAATRPRPARAAEAAIQDAAPLPSIGPGASDEEGRREAEQLRDRILAELKKQ
                     GTAVVCPRPPVVGPTLFLIEVARPRGPVSQLDRAAEDVRHRLAAEADIDLSYEKQGGH
                     RRFIVRRAKPRRVLLGPLLEQKRDWLLQRPGRFIVGQQPDGEVLCADLSDSGTPHLLV
                     AGQSGSGKSVLLQAIVAGLLQYHAPESIRFVLVDPKRVTFNGPSFRSAVSAHLEGPIR
                     AELEDTLPVIQQLVEVMEERYRLFESAQVMDIEEYNEQASQAERLNRRVLVIDEFQDL
                     FTDKAAAQTFFAGVKRLGAKARAAGVHLILATQRPDRDTVPPVIKSNLGGKIALQVAS
                     QPNSRIILDQNGAERLLGKGDLLASLGKGIVRAQAPLLAER"
     gene            complement(5182..7299)
                     /locus_tag="VFQ61_38790"
     CDS             complement(5182..7299)
                     /locus_tag="VFQ61_38790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960501.1"
                     /translation="MSTAFLEAVHADPWAESFLDLPALNASASGAIETNVRHVRELAR
                     AEPHALRSNSLVVLGPPGAGKTHLFARLRRRLGPKAVFVHVRPLVHTEITPRFVLGEI
                     VKQLGYGTHGVSHLPQVHAMVGSLLAHLDGQEGFFPSTFLAECEAMNDADREARLDAA
                     VEQVLSIWQEVDESYLRRLLQVPFSKTPAMQRSLLAWLSGRDCDVTQLARIGATASLG
                     EDLAVAALRTLSVVAALGAPIVIVFDQLENLVDAESASSRLLAYANLTAELVDSMRGL
                     VLVHMALDTEWSRGIEPALNLSQRSRLLMARETLALPSPKQREELLELWSAQLPDRPA
                     ASPWPLTEAQAQRLCRQVGVTPRMLLVEFKRAFRGEAIDEPEGTAADSVAAQSTAAES
                     PPTTSKPQEERLGLASEWEERLTAARTTANEVAEQRSCLDPSRLADGLLACARFLQGA
                     SIGSVKGAEAGQLQLNQGGSTSRLAILHHAHYKSLGSALTKLASVSMREPVIVLREQA
                     HDLPPTWKDTLAKKSALLATGRARWIWLEREDAVSLLALDSLLQGARSGDVTDEQGRP
                     LSESSVAEWVSSTLGVAEWRIIREILNPGEVDEESSQKIDSPRAAPTPSGSAAARSAT
                     TVSEASRADTTSSERASSDPASSGTALALLRRLRIASLDRVVREVIRSDPHATRASVI
                     AELQQNTDEVRWFGRTILGVRVV"
     gene            complement(7313..8185)
                     /locus_tag="VFQ61_38795"
     CDS             complement(7313..8185)
                     /locus_tag="VFQ61_38795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960502.1"
                     /translation="MAKAKAKSPRAASSPDQLLERARTTLQLDGVVKVAALGTATARA
                     ELVARLVSEGFEATKSVVRKPLRSQLLGALAEGAFIRLKSVGSHVAGASVPEAKRAAQ
                     ALVQTGEAKLVVRGSEEVLVSQGASVLARDELKRFAEVAKAVAKVASSKSGASILRSD
                     LAEDLARFMPGGAVAAQSRVRPAEAGSQPSSERVKAETDPMLGRLFSAVDAARDPGTG
                     LSFVPAIVATLSPDLKPEAASALLFSAAQAGLLELRPEGGIQRLSDEELAVCPPGPQG
                     TRLSWARRTNAVVE"
     gene            complement(8524..8805)
                     /locus_tag="VFQ61_38800"
     CDS             complement(8524..8805)
                     /locus_tag="VFQ61_38800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960503.1"
                     /translation="MPRVSRWKTCNGCLAFSTTSTPSIGPGHILLETRFGDFDCLGAI
                     DGNRSYEEASLRVLGLGELLQIKKRTGRPKDLAGIPYIEATIAELSRKT"
     gene            complement(8916..9116)
                     /locus_tag="VFQ61_38805"
     CDS             complement(8916..9116)
                     /locus_tag="VFQ61_38805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960504.1"
                     /translation="MSATEPTQFPMPEVDPNGVDRAQVRRMLALTPIERLRVLESALA
                     SMMKVRDAAQRTQVSRDLASTR"
     gene            9370..9699
                     /locus_tag="VFQ61_38810"
     CDS             9370..9699
                     /locus_tag="VFQ61_38810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960505.1"
                     /translation="MKPAAKIETVSAKTLKRDLRFRAVVLRGMSNDEVLSFLGEIDAP
                     GLSKWIDEALQDAACQLGLSAPLDAWLAERNHVEAELMAERERLYAQQHGYSPSDSEL
                     LRWHREA"
     gene            10122..11003
                     /gene="gspC"
                     /locus_tag="VFQ61_38815"
     CDS             10122..11003
                     /gene="gspC"
                     /locus_tag="VFQ61_38815"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HET9960506.1"
                     /translation="MSSAGYLKPLGGLLIVSMLLVSGFQVTRSLVASWPTWLFPSASH
                     RTRQPTSRPPRSPPHIEAHLAGLTPPVAAKDAPIVISPCQEISASIVTESSDPTWSSA
                     VLKTATEARGRSHRVGDIVAGWKLELVGYNARASSPAVWFSKGRQVCRLDLFSQGAAP
                     VSATSGKMAAATRKGPSAMEGRVRRVSEREVEVDRSVVNWILEDPSTLMGDVRVTPER
                     RDGDSIGLRLVHLGVSGILPTLGLRPGDVVRGVNGFNLANPAQALEAYARLRTASRLE
                     LRLLRGGRELTLEYDIR"
     gene            complement(11062..12063)
                     /locus_tag="VFQ61_38820"
     CDS             complement(11062..12063)
                     /locus_tag="VFQ61_38820"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET9960507.1"
                     /translation="MLKLYFSPLACSLATRIALYEANAEVTFVEVDAETKLTEDGQDF
                     LQINPLGMVPTLELPNGKRLSENSAVLQYVARAYPDARLAPRDGDELAELQRWLSFIG
                     SELHKALFTPLLVGQTGPDAKAYALGLAERRLGYVAQHLAERNLLLDRFSVADAYLFT
                     ILNWSRATPVDLKPWPALVRYQSQLQERSSVARALREEFSLYERERARRAAPGARSPR
                     STAEVIARFNAAFQEHDPTQLEELIADDCVLENTQPAPLGARHVGREACLELWRSIAQ
                     NRDARFQLEEVVVAGDRAQIYWHYVWGPDAGDSVRGVNLMRVREGRIVEARGYVKGS"
     gene            12142..13134
                     /locus_tag="VFQ61_38825"
     CDS             12142..13134
                     /locus_tag="VFQ61_38825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9960508.1"
                     /translation="MKRIDDREHGRAPRPSLRLSDSAAAISPRPQLEVRDLELMIALS
                     MAGSTASAALGLHITQSAVSRALAQVEERLGVALFERRARGLSPTAAGSRLIEGARPI
                     LQQLHELERAVAAPAREPLRLRLVCECYTAYRWLPSVLAKLRERWPDLQVALVTDHTV
                     DPAQGLDAGEIDVALLTTAELPRGRGLREQPLFADEVVFVMAPEHELARGRHVTARQL
                     EQAQLITGDTPPGEARWFMRAAFGRRRPRLKFMHFPLTEAVLDAARAGMGVAVLSEWM
                     ANGYLDRGDLVVRRLAARPLRRPWRIAYRDELSHVAERLKGALCSAVPRLRDAV"
     gene            13402..14631
                     /locus_tag="VFQ61_38830"
     CDS             13402..14631
                     /locus_tag="VFQ61_38830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960509.1"
                     /translation="MQTSSIKSKVVGRAQTLVEQFALRRRSTASIGVVTALLVAASCS
                     TGANDARPGSGDPTASAAESWTGAVSTSCTSGAPQPGQSYFTARLKSTRGRELSLAVL
                     TPKLGDTGVPLSVQLDGKALYEMESHTTQDGVESVQKYHAPLTGIQRVVTIANENSFF
                     ANFDGRETVPANLGTEPQALRFKDGLPPPTVGGDQALIAAMSQLFDSAGRESSACLAA
                     SESNLEANTDSLGSLKNELTTGHRYPVASGVSGSACNGCEYGCKGKYLGCIGAASVLG
                     PFGAAFGALACIGVFERCNSECHKSGTDCCPVGCGGESGILNPIGACCLAGETCVRRA
                     TDDHVALCCEAGTTLCNGVGCCQPGDTCLPSGQCCPEMRNGECCSIGTCTTRADCNGG
                     DPNGSNGCSDNGCCVPG"
     gene            14884..16641
                     /locus_tag="VFQ61_38835"
     CDS             14884..16641
                     /locus_tag="VFQ61_38835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960510.1"
                     /translation="MGFRREIIGGNAQARAGFFVLLLAMGCGATHPGGASGGAGAAGA
                     SGMSTGGAPAAGGHVSSGGSGGAGTTGITGKLGSGGMATLGGGDRMTGGAPMLTEGGA
                     PEGGEPATQGGAPTGGQGGEITMRLPIAKSGQRLKMMGLVANGAEQFQFIQDQKLGVE
                     CLFVEDGTEGGLVCVPSTGYSVVYTDEACQEPALAFGQGTNPPRQMPKYVSDVSAGSL
                     PCAGTFEPYRASFEVKEKISFTPDPQQLVVNRIINGRCQPSLFTAKTPPDSMYRLRRV
                     PDEELVHAHREVTPFSKTLAVGRARAEDGAELTYGTLGLGDEPCALQLDGECVPEPVA
                     HETTANTFLDDECTERASSIRYHGCGTPRYAVRPAPDGVHIFEVGPATKLYKKSAEFQ
                     STTPLCKWGSGGSSVDLSLQREVTGTLPTFGKVVRTGRGPLFLQTSVWENASGSEAYV
                     LAVHQLPQDFLLADGRTCTAREASDGTWRCAIATPGLSFTGSYADEKCEKPLFQSLES
                     QRTDSSTHPTGLMNDAQGKLEAVYSFVPHTGDKLYTMLSGECDALGLSWVPYEVVQYW
                     AAETRTDIADLPRLDLQPL"
     gene            17429..19588
                     /locus_tag="VFQ61_38840"
     CDS             17429..19588
                     /locus_tag="VFQ61_38840"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HET9960511.1"
                     /translation="MFKAVTLLGCLVSSAPAYAAGPTCVAQSCGDGAVFLGSDRSLDG
                     ACNNLQQPDAGAVGDALLYRAGQHYARGPQQTLLFLAEQPHVAGQPNSFPYLPTAAAP
                     AGTCGPPGAAVECEYSISVRELRGYGVQTPLNARVLSNALHSIESPLAPAAPTANAAG
                     LTILWIRTSQIVAHDMQFLQPAVTLSRKRQGFPNQSDPMNATVLTGIPVLEPFDLFNV
                     SPVYTPRGVPEPPIYRALVTEYPEPLLEGHGARTRVGFANSATAFIDGDTLYGRTPEV
                     LDRLREHDGGRFLLESLQSPAVGPLPSFSISGLPPSLAQTGLRDPVLTDGTEPFTPSA
                     ADSRNLSTVGTVVASLIWTRLHNYMAGRCQADMGNSEQASDDALFACARRWTLAVYQH
                     VVFDEFIPAMTGRPLPAYRGYRAWVNPQTSLESVIGPMSLHSTPGELAPIAGKDGAVD
                     ERLQVELPNQPSPPPGYFPFIGSLFPTPTASAGFYLALAGIPTPLGDPTQPGTEWALL
                     EDPVAQIVRGLAFFAHEENDITVIDSQRNIPAGYGLDLVANNIARNLQLGVANYYQAR
                     SEFLHGLDARVYGSGGCPRSAEWDDASDDPPECFERITGDRARAELVRSLLQSPLLGV
                     KAKVKHLPLMTGLLLEPRYASSLVGPTSRAIVEDQFTRVRDGDRFYYRNQMDADEVAW
                     VEAFRMADVVRASIGADVGVQDDVFHVPPPAFFGRGQ"
     gene            19692..20069
                     /locus_tag="VFQ61_38845"
     CDS             19692..20069
                     /locus_tag="VFQ61_38845"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET9960512.1"
                     /translation="MQLAPLICVHDVPAASAWYRYLLGCTSGHGGDEYERLMADERLI
                     LQLHRWDVEHEHGPLGDPDVRPYGNGVILWFELNDFEDAVSRAREMKVETLKERHLSE
                     NGNWEYWLRDPTGYTVVLTSPLP"
     gene            complement(20115..20816)
                     /locus_tag="VFQ61_38850"
     CDS             complement(20115..20816)
                     /locus_tag="VFQ61_38850"
                     /inference="COORDINATES: protein motif:HMM:NF025594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HET9960513.1"
                     /translation="MIDSALTAVVADRIWSLERPVWFGGVRLRARTTVVRLEDGSLLL
                     HTPPPPTDALIHELRALGPVRWLIVPNCWHHLGAPAAAARFPEGKLAGPASALKRNAM
                     LRLDVDLHGGNFGKLIPEFEALPLAGVPFLDETVLYHRPTRTLIGADLVCSAGVGDHF
                     TWRLAGRLTGCYQRVRVPPDARRKLADKAAAARSLKAMMQRPCERLIMGHGDIIEQGC
                     RDQLAQAWRLEGVEI"
     gene            complement(21004..21888)
                     /locus_tag="VFQ61_38855"
     CDS             complement(21004..21888)
                     /locus_tag="VFQ61_38855"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET9960514.1"
                     /translation="MKTKPASWDDLRFLLAVHREKSFFAAGKSLGVAASTVARRVEAV
                     ERSIGRALVHRGNDGTQLDIDALRLVALAEELESRLGALVRDGREAEVSGTVRVSLSE
                     GFVRALVPLLARIQAKHPALRVEVISESRIADLARGEADIGIRIVRSASAPIVSKLLG
                     RASTGLFASREYVERRLSNARLSRDGAGLHDWVGLDASFERLPHEQWMRAYGASRFTF
                     RSNSIIAIEQAIVAGMGMGVLGVVQASSLAGLVKLDLETTPPPVPVFLAFRRDARKTP
                     RVRVVAREIEAEARRLLR"
     gene            22176..22436
                     /locus_tag="VFQ61_38860"
     CDS             22176..22436
                     /locus_tag="VFQ61_38860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960515.1"
                     /translation="MSQHKDSGPRLQVSAGQELTMVYPLGQLTLPSWHVALTSVLGGS
                     VQAVAQRTFPKSSGRQSLVPPAPAAVVSLAPPAPPDAFAGES"
     gene            complement(22869..>23048)
                     /locus_tag="VFQ61_38865"
     CDS             complement(22869..>23048)
                     /locus_tag="VFQ61_38865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960516.1"
                     /translation="RELTTVPAAPLVLVAPAPPEADALSSVDPQPDATNTTTTAASES
                     VIRLDCPARPDFMER"
BASE COUNT         4170 a         7284 c         7286 g         4308 t
ORIGIN      
        1 cgtgttcgac aggcttgcgg cgcataaaac ggtcgccacg cggctcgctg gggacaaacg
       61 ttttgagttc gtcttcctgc ccgcctacgc cccgcagctg aatccggacg aatgggtctg
      121 gcgctacgcc aaacacacgc tgctcggcgg ctgttgcccc tggggctatg atgatttgca
      181 gaacgcggtt catagtgcac tgtcgtcttt caagtcccat tctgatctgc ttgcgagctt
      241 ctttcgtggc gcccgactgt ctctttagcc caatgaatta ccagtaaaga cgccgcgctc
      301 tctgagggag cgacgtcgct cagaatcgag acgagccgaa taattgaggc gagcgggcgc
      361 cttcagagca ttctccggaa gttgggattc gccacccgat cgaacggcac aatgtattat
      421 actggaggcc tctaatggcc aacgttcgta cgcacgcaaa tcggaatctt cgaccgagag
      481 ttctcgcgtc gggcgagaga cgtggcatct cgaactactt tgctatcagc gagggtcagc
      541 tacccaactg gtgggcagaa ttccaggaaa gctacgatgg tgaggtcctc ggcgcaattg
      601 agaacagacc tggcaacaaa gaagaggcta ttatcctgac gagcgaagag gtgcttgtgc
      661 tggaaggacc tcgtgtgcgc cgtttgcggt acgatgaagt ggcctcaccc gggcgtcttg
      721 tgaaggcgcc ctttccagag aagatcagcg ctgtaagaag gtccggggaa atttttgagt
      781 taccaattcg gggtaggacg ggggcaatag gcgatgtgac gcgattcctc ttgtgcgcgg
      841 tccgcgaatc tcaggtggtt tgactgtttg actgtttgac tcaggcgtca tggaggcggg
      901 ccggagcgcc gatcgtgatg tccgcctctc aggctcgcgg aaggacccga ggcccgcgcg
      961 acgcgcacag cgggtctcgg ggcgacatcc tgcctcaact tcacgaacat tcagattggt
     1021 gaactggctg cggccggtac gccagttgcg gccgccgccg caacatatgc cgctcgaggc
     1081 gattaagatg ctaaccttcc caatcctcat gctgtttgga gaacatgtcg cttcgttcaa
     1141 ggacgaagca gcgcttaggc gttctctgtc tctggttaca gacaagggct taaagaacgg
     1201 tttcatcggg gacaccatcc tggtggatag cgacctgaat caacatgcca tactggcctt
     1261 tcagctcaaa gggcgagagc cgctattcgg ttggttgcgc tcaggccggg agatcattca
     1321 gtttgaaacg acgcctgctc gccgcacaac gcttccagac attaagcgcc ttcttttaaa
     1381 aagaatctcg caatccgaat gggaagatgt tccgagcctg gtggctgcaa ttgagtctgc
     1441 cactgaattc tctgcccttg ttgagccttt tgcggtctcg ttctaagctt cgtattgctc
     1501 ggtgacggcg attttccccg ttcctaaagt ctgctccgtt gaagtgaggg ccacccgaag
     1561 tgacatccgc tgacggctgc ttgcaaagcc cgcatcgaaa gcgagcgtct cgccaaccgt
     1621 aacagcattt cgcgggcaag gcttcgctgg cctcactctc cgcaggagcg cgctgcacct
     1681 gctgtctcgt gcccgtcggc aggcactcgc atcatggccc gagccacttg gcccacccag
     1741 cggcgcccct cacgaactcg cagggtcttt gggttgaagc tatccgcgac gattgcgaat
     1801 ccgtcggcgc attcgggaac cggccaacgc gagtgcggcc aacatccccg gcagcgtgag
     1861 gccggagctc gatgaactgc ccatggtgca gccgcggtcc ctctcgtcgg acgtgattac
     1921 ctcatcgaaa ttcaagcccg tgcttccagc ctgcccgacg agcgaggggc aggcgttgag
     1981 ggcggcttca caggcttcga gcgggtattg ttcggcgcag tcgtggtatc tgcgtggctg
     2041 cgcatacgtc ctgcaccaga catcctcgta accggcaggc ggcacggtac attgcctaaa
     2101 cagatccact tgggcgttcc cgagagctag ctcgagatcg ggtgcggtaa cctctgctcc
     2161 ggagatgcaa tcttgggcga cggtctggac ggcaccggtc gctaaatcaa gggcctcgat
     2221 ttcatagcag tacttttcgg caagcgcgaa tgacgcgaac agatgattcc ccaccatcca
     2281 tctttcaccg tcctgcggct gaacgcgata caaatactgt gaaccgtcag cgcgttccgg
     2341 tgtctcgagc aaggcattta ccacgccgtg cacgctgctc ggaaccaagt agcacggaag
     2401 ctcgttacag ctatctctct ctccctcttt acagcacacg gtctcgcctt ctagcaccgc
     2461 cctcgcagca atatccgtca cgtgccagct ggacgaaggt gtcgtgggca gcgttacggc
     2521 ctggaagatg tgctgagcag cctcgtattc gagctcctca gtgtcaagga gcgcaaccga
     2581 gtagctctcg ccttctgcca agttcgtttg gctcttccaa accagccaag tgtcaccctg
     2641 caccaatagc gtttggacaa cgttcccggt cacgacgttg ccgctcgcgt cggagacctc
     2701 gaatatgggg tctgtggggc acgtcgtatc gcacgaagta cgaatcaaca cgacgccatc
     2761 gacgggcaca ctcgagggga cctcgaagaa cagagtgtct cgagtgggtg gggccgaaca
     2821 cgctgccgcc ggcgctgtgg tatgcaggta acccgtagcg gccaaagcag cgaccagagt
     2881 tggaagggta cggactctca tgatgaattc ctgcggcgac gggtgacctt cgcgactctg
     2941 caaagtgcgg atggtcgtgc ggcgctgttg accagcacga gcaagaatag cgccagctcc
     3001 cgtcgggagg aacctagcca gtttcgcgag gtcgctcgag atcgaactga cagcgacagc
     3061 gtagagtggc acagattggg cctagccagc gcattaccct cgtcggtttc cgactcgatc
     3121 tgctggcgcg cttacggagc cttgacggac cgctccagag cgatggtcgc gagtaagtat
     3181 tcggccctca tcgctcggcc aacagcggcg cctgcgcccg aacaatgcct ttacccaggc
     3241 tcgcaagtaa atcgcctttg ccgagcagcc tctccgcccc attctgatcg aggataatcc
     3301 gcgaattggg ctgggaggca acctgcagag ctatcttgcc gccgaggttc gatttgatca
     3361 ccggaggaac ggtgtcgcga tcgggtcgct gcgtcgccag gatgaggtgc acgcccgccg
     3421 cacgcgcttt ggcgcccaat cgcttcacgc cggcgaagaa tgtctgagct gccgccttgt
     3481 cggtgaatag atcctggaac tcgtcgatca cgaggacgcg ccggttcaag cgctccgctt
     3541 gactggcctg ttcgttgtac tcctcgatat ccatcacttg cgcggactcg aatagacgat
     3601 accgctcttc catgacctcg acgagctgtt gaatgaccgg aagtgtgtcc tcgagttccg
     3661 cccgaatcgg cccttcgaga tgcgccgaga cggcggatcg aaaagacggc ccattgaagg
     3721 tcacccgctt ggggtcgacc aggacgaagc gaatcgattc gggggcgtga tactgaagga
     3781 gccctgcgac gatcgcttgt aagagcaccg atttgccgct gccactctgc cccgccacca
     3841 gcaagtgggg tgtgcccgaa tcggatagat cggcacagag cacttcgcca tcgggttgtt
     3901 gaccgacgat gaaacgtcca ggacgctgga gcagccaatc tcgcttctgt tcgagcagag
     3961 gcccgagcag cacgcgccga ggcttggcgc gcctgacgat gaagcgacga tgcccgccct
     4021 gcttttcata gctcagatcg atgtcggctt cggcggccaa gcggtggcgc acgtcttctg
     4081 ccgcgcgatc gagttgactg acgggccctc gcgggcgcgc cacctcgatg aggaacaacg
     4141 tcggacccac caccggcggg cgcgggcaca cgactgccgt accctgcttt ttgagctccg
     4201 ccagaatgcg atcacgcagc tgctccgcct cacgccggcc ctcctcgtcg ctcgctcccg
     4261 gtccaatgga cggcagcgga gccgcgtcct gtatggcggc ttcggccgct cgcgcgggcc
     4321 gcggtcgcgt cgccgccgcg ggcggatcgt cgcgcacggc caatcgctcg gggtacgtct
     4381 cggcgcactc gcgcgcgacg gggcacgccg agcacaagtc cttgcgatgc gtgggagggg
     4441 cgctctccgg cgtcgcgagc caactcgcca tttggtcgag cagcggttgc actacgcgat
     4501 cgaccaaagc atcggcagat tccgcggtca gcgccgtctc tcgcagggtg ggagtgaagc
     4561 gcaggataac gggggtcgcg tcgccgccgt ccgaagcccg gagtaaggag cggtaaagag
     4621 ccacctgcgc acggtccatc tcgtcattgg cctcgtcggt gagcttgtat tcgaccacct
     4681 cgagattgcc ccgggcatca ctatacaaag catctaggcg accgtgaacg acggggccat
     4741 cttcctggac tcgagcttcg acggcgcgtt caccagtgcg caccacctgt ggtaacgccg
     4801 ctcccgggcg gaccgcgaag ccttgtaaac gccccgcaag gtggcgggca aactcgcgca
     4861 gcgcctccgc gaggtcgtcc acctcaccag gcatggtggc gtacgcgacg tcggcttcga
     4921 gctcgcgaaa caggtaatcg agcagccact gcgccaagtg cgcctcggtc tcatcgcgtg
     4981 gcgttccggg ctgcagggac gcaaagtgcg caggcggatg ctccacgtct cggctgaaga
     5041 gctcgacgat gcgatgaaaa gcggcaccca agcacgaaga gccgaccggg aatgccaccg
     5101 cctgctgata caatctcccg agcgcaaaga tgcgcgggca gcgcagcgcg ttgcgcacct
     5161 ccgtgacact gatcctctcg ctcataccac cctcaccccc aagatcgttc gcccaaacca
     5221 gcgcacttcg tccgtattct gctgcagttc agcgatcacc gaggcgcgcg tggcgtgcgg
     5281 atccgatcgg atcacctctc gcaccacccg atcgagcgac gcaatccgta gtcggcgcag
     5341 cagcgccagt gccgtccccg aactggctgg gtctgaactg gctctctcgg aactggtcgt
     5401 gtccgcgcgt gaggcttccg acaccgtcgt cgccgaacga gcggcagccg agccactggg
     5461 agtcggggcc gcacggggag agtcgatctt ctggctcgat tcttcatcca cctctccagg
     5521 attcagaatc tcgcggatta tccgccattc ggcaactccc aatgtggatg acacccactc
     5581 cgcgacagaa ctttcgctca ggggtcggcc ctgctcatcc gttacgtccc ccgaacgagc
     5641 gccctgcagc aatgagtcca acgcaagtag agaaacggcg tcctcacgct ccagccaaat
     5701 ccagcgcgct cgacccgtcg cgagcaacgc gcttttcttg gcgagcgtgt ccttccaggt
     5761 cggaggaaga tcgtgcgctt gctctcgaag cacgatcacc ggttctcgca tagagacgct
     5821 ggcgagcttc gtcagcgcac tacccaggct cttgtaatga gcgtggtgca agatcgcgag
     5881 tcgtgaggtg gagccaccct gattgagctg aagctgcccg gcctccgcgc ccttcacgga
     5941 tccgatgctc gcaccttgaa ggaagcgtgc acacgcaagc aagccatctg ccagccggga
     6001 tggatcgaga cacgatcgct gctccgccac ttcgttcgcc gtcgtgcgcg cggccgtcag
     6061 ccgctcctcc cattcggagg ccaagcccag ccgttcctcc tgcggcttac ttgtggtcgg
     6121 tggcgactct gccgcggtcg actgtgccgc taccgagtct gccgcggtcc cctctggctc
     6181 gtcgatggcc tcgccgcgaa atgcccgctt gaactcgacg agaagcatcc gcggggtgac
     6241 ccccacttgc cggcacaagc gttgcgcttg cgcttcggtc aaaggccaag gcgacgcggc
     6301 gggccggtcc ggcagctgtg cggaccacag ctcgagcagc tcttctcgct gtttcggcga
     6361 ggggagcgcc aaagtctcgc gggccatgag cagccgggaa cgttgggaga gattcagcgc
     6421 cggctcgatc ccgcggctcc actcggtatc gagcgccatg tgcacgagca ctaggccgcg
     6481 catcgaatcg acgagctccg ccgtcaagtt ggcgtaggcc agcaaccgcg aactcgcgct
     6541 ctccgcgtcc accaagttct cgagttgatc gaacacgatc acgatgggag cgcccaacgc
     6601 cgccaccacc gatagcgtcc gcaacgccgc caccgccaag tcttcgccca gcgaagcggt
     6661 ggcaccgata cgcgctagct gagtcacatc gcaatcccgc cccgaaagcc atgcgaggag
     6721 cgagcgctgc atggccggcg ttttggaaaa cggcacctgc aacagccgcc gcagatagct
     6781 ctcatccacc tcctgccaga tcgaaagcac ctgctcgacc gccgcgtcca gccgcgcttc
     6841 gcggtcggcg tcattcatcg cctcgcactc cgccaagaac gtgctcggga aaaagccctc
     6901 ctgaccgtcc aagtgcgcca gcagcgagcc caccatggcg tgcacctgcg gcaggtgaga
     6961 aaccccatgc gtcccgtagc cgagctgttt gacgatctcc cccagcacga agcgcggcgt
     7021 gatctcggtg tgcacgagcg gccgcacgtg cacgaacacg gccttgggcc cgagcctgcg
     7081 ccttagccgc gcgaacaaat gcgttttgcc cgcacccggt ggacctagca ccacgagcga
     7141 gttcgagcgg agcgcgtgcg gctcagcgcg agccagctcc cgcacatgcc gcacattggt
     7201 ctcgatggcg ccactcgccg aagcgttcag cgccggcaga tccaaaaaac tctccgccca
     7261 cggatcggcg tggacggcct ccaagaacgc cgtactcatc gtgccacctg catcattcca
     7321 ccaccgcgtt cgtgcgcctc gcccaggaga gccgcgtccc ttgtggcccg ggcgggcaca
     7381 ccgccaactc ctcgtcggag agacgctgaa ttccgccttc cggtctgagc tccaacaatc
     7441 ccgcctgagc cgccgagaac aacagcgcgc ttgcggcctc gggcttcaaa tccggactga
     7501 gcgtcgccac gatggcgggc acgaacgaca gccccgtgcc tgggtctcgc gccgcgtcca
     7561 ccgccgagaa caagcgcccc agcattggat cggtctccgc tttcacgcgc tcactcgacg
     7621 gctgagaccc agcctcagcc gggcgcacgc gtgactgcgc ggcgaccgct ccgccgggca
     7681 taaagcgcgc taaatcttcc gccaagtcgc tgcgcaaaat cgaagctcca ctcttggagc
     7741 tcgcgacctt cgcgaccgcc ttggcgactt cggcgaaccg cttcagttca tcgcgcgcaa
     7801 gcacggaggc gccctgcgag acaagaacct cttcgctccc tcgcacaacc agcttcgcct
     7861 cacccgtctg gaccaacgct tgtgcggcac gcttggcttc cggcactgac gcccccgcca
     7921 catgtgaacc gactgacttg agtcgaatga aggcgccctc cgcgagcgcg cccagcaatt
     7981 gcgaccgaag cggcttgcgc accacggact tggtcgcctc gaacccctca ctgacgagtc
     8041 gagcaacgag ctccgcgcgc gcggtggcag tcccaagcgc tgccaccttc acgactccat
     8101 ccagctgcaa cgtcgtgcgc gccctctcca acaattgatc tggactagag gctgcgcgcg
     8161 gcgacttcgc tttggccttg gccatggcac ccaagatacg cggaccgggc agcgattgca
     8221 gtgcctttct tgacgtggcg cgcgcctcgc ggcgacccat gattctcgtc gctctgaggg
     8281 gttcgcgagt gcgaccgccg ccccggggtt tacgggcgcg aagcgcgtcc cagggcgtcg
     8341 aacgtccccc acccactccc gctgtacgtc aagatgccgc gcacgcggca ccctgtaact
     8401 ccaaaccgtg ctccgctcgc gacgccatca agacttcagg tgcgcttgcg ggcgatccgt
     8461 ccaaggacat gcgctccgga gcgctgatga gctctatcga tcaacgaatg cagtccggca
     8521 acgctaggtt ttgcgcgata gctctgcgat cgtggcctcg atatagggga tgccggcgag
     8581 atccttcggg cgtcccgtgc gcttcttgat ctgcagcaac tcaccaagcc caagcacgcg
     8641 cagcgacgcc tcctcgtagg aacgattgcc gtctatcgct cccagacagt cgaagtcgcc
     8701 gaagcgcgtc tcgagtaaga tgtgaccagg gccaatcgac ggcgtcgatg tcgtcgagaa
     8761 cgccaagcag ccgttgcacg ttttccatcg acttactcga ggcatctgta gccatcgaac
     8821 tcgatgatcg gcgatgcacg acgtcgaggt cccaggtcat cgtgggcgcg ccttgggccg
     8881 ccgccatgcc tacgacaatc gcctccacac ccgcgtcagc gagtcgaagc aagatctcgt
     8941 gatacttggg ttcgctgagc ggcatctcgc accttcatca tcgaggccag cgcagactcc
     9001 agcacgcgca accgctcgat gggcgtaagc gctagcatgc gacggacctg cgcgcggtcc
     9061 accccgttgg ggtcaacctc cggcatcgga aactgggttg gttcggtcgc gctcacgacg
     9121 ccactatacc agcacgattg ggcgcgtgca ggccggtttc tgtcgagtgc agagtgcgcg
     9181 cggcacgcgc gtgggagcct tgcaaggact tcgtcctggc tccggcgacg acgtgcaatt
     9241 cgttgggcgt gtctgtttgc gctcgagatg gccgaagcag tcgggggaaa cgccgaagcc
     9301 tgcttgaaca atgacgactc tcgcgtaaga tggctggccc tatatcaagt ggtcagttcg
     9361 gagcgaacaa tgaagccagc tgccaagatc gagacggtct ccgccaagac tctgaagagg
     9421 gacctgagat tccgagcagt ggtgctccgc ggaatgagta acgacgaggt gctctcattt
     9481 ctcggcgaga tcgacgcgcc tggcctttcg aaatggatag acgaagcgtt acaggacgcc
     9541 gcctgtcaac tgggcctaag cgcgcccctg gacgcctggt tagcggaacg caaccacgtt
     9601 gaagccgagc tcatggccga gcgggagcgc ctttacgcgc agcagcacgg gtactcaccg
     9661 agtgactccg agcttcttcg ctggcaccgc gaggcctgac acgaggactt tcaacccgcg
     9721 tcgcgctcgc gctgcgtttg atccctgcaa ggctccaagc gcagtcctag agactaaacg
     9781 cctacgctga gcatgagtcg cggcgatggg cgtaatgcca gtcgaaattg ggaagcgcgc
     9841 acgagctctt gagtgcagtt tgcgtactcg ccctagccgc ggagaagtcc gcccggagtt
     9901 cgggcgagct cagtcacagt tcgggtcggt gcgagcacgt tcgctcctcg gtggcgcgcc
     9961 aaacgcggct caacatttca tctgccaatt tgtcgtggcg tgcgcacggg gatgggttgg
    10021 tccttgacag gacgtcattg ggccttcttt gatcgcaatt cagactgagt ttttgcggcc
    10081 acttctggcc tttggcacgc gcttcgctct agccgtgcga catgtcgagt gctgggtacc
    10141 tcaagccgct cggggggctt ttgatcgtga gcatgctgct agtcagtggc tttcaggtga
    10201 ccaggagcct cgtcgcgtca tggccgactt ggctgtttcc tagcgcttcc caccgcacgc
    10261 gccaaccgac atcgaggcca cctcgcagcc caccacatat cgaagcacac ctcgcgggcc
    10321 tgacaccacc tgtggctgcc aaagatgctc cgatcgtgat ctcgccgtgc caggagattt
    10381 cggcgtcgat tgtcacggag tcgagcgacc cgacgtggtc gagcgctgtt ttgaaaacgg
    10441 ccacggaagc acgcggccgc tctcatcgag ttggcgacat cgtggcgggt tggaagctcg
    10501 aactggtggg gtacaacgcg cgtgcgtcgt ccccggcggt gtggttttcg aaagggcgac
    10561 aggtgtgtcg gctggatctg ttctctcagg gtgccgcccc cgtgagcgcc accagcggga
    10621 aaatggcagc cgctacacgt aaggggccca gcgcaatgga aggccgtgtg cgccgagtca
    10681 gcgagcgcga ggtggaagtc gatcgcagcg tggtcaactg gattttagaa gatcccagta
    10741 cgctcatggg agacgtgcga gtcacgccgg agcgacgtga cggtgactcg atcggcctgc
    10801 gtttggtaca tctgggtgtg agcggcattc tgccgaccct cggtttgcga ccgggcgacg
    10861 tggttcgcgg cgtgaacggc ttcaacttgg caaatcccgc gcaagcgctg gaagcctacg
    10921 cgcggcttcg cacggcttcg cggctcgaat tgcgactcct gcgcggcggt cgcgagctga
    10981 ccttggaata cgacatccgc tgaggggctt ccgctccaat ccacgcgacc gcgcgctcgg
    11041 ttctcgcccg cagccgcgtg cttagcttcc cttgacgtac ccgcgcgcct ccacgatgcg
    11101 tccttcacgg acgcgcatca agttgacgcc gcgcacggag tccccggcgt cgggtcccca
    11161 gacgtagtgc cagtagatct gggcccgatc gccggcgacg accacctcct ccaattggaa
    11221 tcgggcatcg cggttctggg cgatggaccg ccacagttca aggcaagcct cgcggccaac
    11281 atggcgggcc ccgagcggcg ccggttgggt gttctccaac acgcaatcat ccgcgatgag
    11341 ctcctcgagc tgagtggggt cgtgctcctg gaacgcggcg ttgaagcgcg cgatcacctc
    11401 cgcggtactg cgcggtgagc gtgcgccagg ggccgcccgt cgcgctcgct ctctttcgta
    11461 cagcgagaac tcctcccgta gcgcgcgagc cacgctggac cgttcttgca gctggctttg
    11521 gtaacgaacg agcgctggcc atggcttcaa atccacgggc gtcgcccggc tccagttcaa
    11581 gatcgtgaac aggtaagcgt cagctacgct gaatcgatcg aggagaagat ttcgctcggc
    11641 gaggtgctgc gcgacataac ccagccgccg ttcggcgagg ccgagcgcgt aggctttggc
    11701 gtcgggccct gtctgcccga ccaatagtgg cgtgaacagc gccttgtgca gctcgcttcc
    11761 gatgaagctc agccagcgtt gcagctccgc gagttcatcg ccgtcgcggg gagcgagccg
    11821 cgcatcgggg taagcccgcg cgacgtactg caacacggcc gagttttcgc tcagccgctt
    11881 gccgttcggc agctctaggg tcggcaccat tccgagcggg ttgatttgta ggaaatcctg
    11941 cccgtcctcg gtgagcttgg tctcggcgtc gacctcgacg aacgtgactt cggcgttggc
    12001 ttcgtacaaa gcgatacggg tcgcgaggga gcaggcgagc ggggaaaaat agagcttcaa
    12061 catcgggcac tgacctccat ggccccaagt tgccccaatc cttgatatgc ggccaacgca
    12121 ttgtcgtgct aggagcaatg agtgaaacgc atagatgacc gtgaacacgg tcgggctccc
    12181 aggccttcgc ttcggctttc ggactcagcg gccgcgatct caccgcgccc gcagctcgag
    12241 gttcgggatc tagagctgat gatcgcgctg tcgatggcgg gaagcaccgc gagcgcggca
    12301 ctcggtttgc acatcacgca gtcggcggtg agccgcgcgc tcgcgcaggt ggaggagcgc
    12361 ctgggagtcg cgttgttcga gcgccgggcg cgtgggctct ctcccaccgc ggccggctcg
    12421 agattgatcg agggagcgcg gcccatcttg cagcagctcc acgagctaga gcgggcggtt
    12481 gccgcgccgg cgcgcgagcc gttgcgacta cgcctggtgt gcgagtgtta caccgcatac
    12541 cgctggctgc cgtcggtgtt ggcgaagctg cgggagcgtt ggccggactt gcaggtcgcg
    12601 ctcgtgaccg accacacggt ggaccccgcg cagggactcg acgccggtga aatcgacgtc
    12661 gcgctgctta ctaccgctga gttgccccgc gggcgagggc ttcgggagca gccgctcttt
    12721 gcggacgagg tcgtgttcgt catggcaccc gagcacgagc tggcccgcgg tcgtcatgtc
    12781 accgcgcgcc agctcgagca ggcgcaattg atcaccggcg atacgccacc cggcgaggcg
    12841 cgctggttca tgcgtgcggc tttcggtcgg cggcgcccgc ggctcaagtt catgcacttc
    12901 ccgttgaccg aggcggttct cgatgccgcg cgcgctggca tgggcgtcgc ggtgttatcg
    12961 gagtggatgg cgaacggcta cttggatcga ggagacttgg ttgtccggcg tctggccgcg
    13021 agacctctgc gccggccctg gcgcatcgcg tatcgcgacg agctcagcca cgtcgcggag
    13081 cggctcaaag gcgcgctctg cagcgccgtg ccccgcctgc gcgacgcggt ttgaccacta
    13141 tgctctgttt ctcgtcccag ggctcgcgct cacctcggac tccaccgcgt aacgcgaacg
    13201 ttcgcgaacg ttcggctttt ggcagggcgg tggttgcgac agcgcagctt gcggtgcggg
    13261 catcgaaaac ggcgattttt tgccggttca gcgaagggcg gggttttacg tgccgattgc
    13321 tccagcatcg gtacgccgcg tgctgagggg cggtgtatgc acgcctcttg ggttggggcg
    13381 tgtgaacgca tcggagtggt catgcaaaca tcgtcaatca aatcgaaggt cgtggggcgg
    13441 gcgcaaacgt tggtagagca gttcgcgttg aggcggcgct cgacggccag tatcggggtg
    13501 gtcaccgcgc tattggtcgc ggcgagctgc agcacgggcg cgaacgacgc tcggccgggt
    13561 agcggcgacc cgaccgcatc ggcagcggag agctggaccg gggccgtgtc tacgagctgc
    13621 accagcggag ccccgcaacc cggtcaaagt tacttcacgg ctcggctcaa gagcacgcgc
    13681 ggccgagagt tgtctttggc agtgctcacg ccgaagttag gcgatacggg agtgccctta
    13741 tcggtccagc tcgacggcaa ggcgctgtat gagatggaat cccacacgac tcaggacggc
    13801 gtggagagtg tccagaagta ccatgcaccg ctgaccggca ttcagcgcgt ggtgacgatt
    13861 gcgaatgaaa acagcttctt cgcaaacttc gacggacgtg agacggtacc ggcgaacttg
    13921 ggtaccgaac ctcaagccct ccgcttcaaa gacgggttgc ccccgccgac cgtgggaggc
    13981 gaccaggccc tgatcgcggc catgagtcag ttgttcgact ctgccggtcg agagtcttcc
    14041 gcgtgtcttg ccgcatccga gtccaatctc gaggcaaaca ccgattcact tggatcgctg
    14101 aagaacgagc tcacgactgg gcaccgttac ccggtggcct ccggtgtcag cggcagcgct
    14161 tgcaacgggt gtgaatatgg ttgcaagggg aagtatttgg ggtgcatagg cgccgcctct
    14221 gtgctcggcc cgttcggcgc ggcctttggc gcgctcgctt gtatcggcgt attcgagcgg
    14281 tgcaatagtg aatgtcacaa gtccgggacg gactgttgcc ccgtgggttg tggcggggaa
    14341 agtgggatcc tgaacccgat tggcgcttgc tgcctcgcgg gcgaaacgtg tgtgcgacgc
    14401 gccaccgacg atcacgtggc cctgtgctgt gaagcgggca cgacactctg caacggagtc
    14461 ggttgttgcc agccgggcga tacgtgcttg ccctcgggcc aatgttgccc cgaaatgcgc
    14521 aacggcgaat gctgcagcat cggcacctgc acgactcgag ccgactgcaa tggcggcgac
    14581 ccgaacggct cgaacggctg cagcgacaac ggctgctgtg ttccgggcta atgtgagttg
    14641 ccagacgacg agttgccgga gctagagcag ccgtaaattg cagtggctcg agtccatggg
    14701 ccactggagc cgttgaaagg cgagaggagc atcacgaacg tgctcctctc gcgcttcgag
    14761 cacgtgcggc aaactttccg gtgctccttt cgcgtcgaac ccgtcagcca tgcccgttgc
    14821 gcatactcga cgataagata tctcgattgg acgggcgcgc gtagactgcc tatcgtcgcg
    14881 cacatgggct ttcgacggga gattattggc ggaaatgctc aggcgcgcgc ggggtttttc
    14941 gtgctgctgt tggccatggg gtgtggggca acgcatccgg ggggagcgtc cggaggcgcc
    15001 ggagcggcgg gcgcgagcgg gatgagtaca ggtggggcac cggcagctgg gggtcacgtg
    15061 agttctggcg ggagcggtgg cgccggcacc acggggatca cgggcaagct cggctctggg
    15121 ggcatggcga cgcttggcgg tggcgaccgg atgacgggtg gcgcgccaat gctgacggag
    15181 gggggcgcgc cggaaggggg tgagccggcc acgcaaggcg gcgccccgac gggtgggcaa
    15241 ggcggcgaaa tcaccatgcg cctgcccatc gcgaagtccg ggcaacgatt gaagatgatg
    15301 gggctggttg cgaacggggc ggagcaattt caattcattc aggatcagaa gctcggcgta
    15361 gagtgcctgt tcgtggagga cggcaccgag ggcggcttgg tttgtgtgcc atccacgggc
    15421 tactccgtgg tgtacacgga cgaagcctgc caggaacccg cattggcgtt cgggcaggga
    15481 acaaatcccc cgcgacaaat gcccaagtac gtgagcgatg tgtctgccgg atccttgccg
    15541 tgtgccggca cattcgaacc gtaccgggca agcttcgagg taaaggagaa gattagtttc
    15601 actcccgatc cacaacagct ggtcgtcaat cgaataatca acggccgctg tcagccatcg
    15661 ctatttaccg ccaagacgcc gcctgactcg atgtatcggt tacgccgtgt tccggacgag
    15721 gagctggtgc acgcgcaccg cgaagtcact cctttcagca agaccttggc ggttgggcgc
    15781 gcgcgtgccg aggacggagc ggagctcacc tatggcactc ttggcctcgg agatgaaccg
    15841 tgcgctctac agctggatgg cgagtgcgtt ccagagccgg tcgcgcatga aacaacggcc
    15901 aacacctttc tcgatgacga gtgcaccgaa cgggcctcga gtatccgcta ccacgggtgc
    15961 ggaacacctc gttacgccgt tcgcccggcg cctgacggcg tccacatttt cgaggtaggc
    16021 cccgccacca agctttacaa gaagtcggcc gagtttcagt caaccacacc gctgtgcaag
    16081 tggggttccg gaggatcgag cgtggatttg tcgctacagc gtgaggtcac gggaacgctg
    16141 cccaccttcg gtaaggtggt ccgaaccggg cgggggcctt tgtttctcca gacttcagtc
    16201 tgggagaatg cttcgggatc cgaagcttat gtgttggctg tgcaccagct gccgcaggac
    16261 ttcttgctgg cagacgggcg cacctgcacg gctcgcgaag cgagcgacgg gacctggcgt
    16321 tgcgccattg ccacgcccgg cctctccttt accggcagtt atgccgatga aaaatgtgag
    16381 aagccgctgt ttcagtccct tgaaagccag cgcaccgact catccacgca tccaaccgga
    16441 ttgatgaacg atgcgcaggg caagctcgaa gctgtttact ccttcgttcc gcacaccggc
    16501 gacaagctgt acaccatgtt gagtggagag tgcgatgcgc tgggcctgtc ctgggttccg
    16561 tacgaagtag tgcagtactg ggccgccgaa actcgcaccg acatcgccga tctgccgcgc
    16621 ctcgacctac agccgctcta gcgtcgtccg cagccccggc tcacctgtct ctgcgaattg
    16681 ccagacaaaa ccatggagcc gaccgttcac gtagggttcc agtcgaggtg ccgttcgttg
    16741 ttgcgtgcgg cgcgcgcggt agacggaggc gacccccgag cacgcgactg cgccatgaag
    16801 cggccgccgc cgcgtagtcg cgcaagttac atgggggccg ccaaacagcc acagccatgg
    16861 atgtcatcac tcagggaaat tgcctattcg cttgcgggac cctcgagcgc tatcttggca
    16921 gtgtttccgc cccccaagta acgtgcgcga agctcgtttc ttccgcacgc tagcgcagtc
    16981 atagcggccc atggaacgcg ctccaccgcg ctcgggccaa catcgactct attctcgctg
    17041 gaccgaaccc ctcatccaat ctctataccg agcacgcagc tgttcgcgcg ctctcatact
    17101 tttgatttcg ccgggctcag cgggctccgc gctgtgacag gttgtggaag agacagattt
    17161 ttgccgaccc gcggtgctgg cctgtgatag ggtcactccc tgcaaaaggc tgccccccgg
    17221 agctgtctcg ggtgcgcagg cttaggaggg aagccacgtg ataaaggtac cgtttgcaaa
    17281 agggctcggc gcgatgggta ttgggtccac cgtggacgac ggtgttgcag gcaggcaggt
    17341 ttgcggaagc gctggttcaa gcggtagggc ttcaagtcgg gtgagagcga gcggtgccag
    17401 cgtgcacggg cttgtattga gtggttccgt gttcaaggcg gtcaccttgc tgggttgcct
    17461 cgtctcatca gctccagcct atgcggcggg gccgacttgc gtggctcaga gttgcgggga
    17521 tggagccgtg tttttgggga gtgaccgctc actggacggg gcttgcaaca acttgcagca
    17581 gccggacgcc ggagctgtgg gggatgcgct tttgtatcga gcggggcagc actatgcgcg
    17641 cggtcctcag caaactctac tcttcttggc tgagcaacct cacgtcgctg ggcagccgaa
    17701 ttcctttcct tacctgccca cggcggctgc cccagcgggg acatgtggcc cgcccggtgc
    17761 agccgtggag tgcgaataca gtattagcgt gcgcgagttg cgcggttacg gcgtgcaaac
    17821 tcctttgaac gcacgtgtgt tgtcgaatgc tttgcatagc atcgagtcac ctcttgcgcc
    17881 ggccgcgccc accgccaacg ccgcagggtt gaccatccta tggattcgca cctcgcagat
    17941 cgtcgcgcac gacatgcagt ttctgcagcc cgcggtgacg ttgagccgca agcgccaggg
    18001 ttttccaaac cagtccgacc cgatgaatgc gacggtgttg acgggtatcc cggtgctcga
    18061 gcccttcgat ttgttcaacg tcagcccggt ttatacgccg cgcggcgtac cggagccgcc
    18121 catctaccgc gctctcgtta cggagtatcc cgagcccttg ctggagggtc acggcgcgcg
    18181 cacgcgggtg ggcttcgcga actctgccac ggccttcatc gacggtgaca ccctgtacgg
    18241 aagaacgccc gaggtgctcg atcggctgcg cgagcatgat ggggggcgct tcttgctgga
    18301 gtcactgcaa tcgcctgcgg tgggaccttt gccgtcgttc tcgatctcgg gtctacctcc
    18361 tagtttggcg caaaccgggc ttcgcgaccc tgttttgacg gacgggacgg agccgttcac
    18421 gccgagcgcg gccgactccc gcaatctgag tacggtaggc acggtggttg ccagcctgat
    18481 ttggacgcgg cttcacaact acatggctgg gcgttgtcag gcggatatgg gcaactccga
    18541 acaggcgtct gacgacgcct tgtttgcgtg cgcgcggcgc tggacgctgg ctgtctatca
    18601 acatgtcgtg ttcgacgagt tcattcccgc catgacgggc cgtccgctgc cggcttatcg
    18661 cggctatcga gcttgggtaa atcctcaaac gtcgctcgag tccgtgattg gcccaatgtc
    18721 gctgcacagc acgcctgggg agctggcgcc gattgccggc aaagacggcg cagtggacga
    18781 gcgactgcaa gtcgagttgc ccaatcagcc aagcccgccg ccaggctatt tcccgttcat
    18841 cggctcgttg tttccaacgc ctaccgcgtc ggccggattt tacttggcgt tggctggcat
    18901 cccaacgccg cttggggatc ccacgcagcc tggcaccgaa tgggctctgc tcgaggatcc
    18961 agtcgcgcaa atcgtgcgag ggcttgcgtt cttcgctcac gaggagaacg acatcacggt
    19021 cattgattcc caacgcaaca ttccggcggg ttacgggctc gacttggtcg ccaataacat
    19081 tgcccggaat ctgcagctcg gggtcgctaa ctattatcag gctcgctccg agttcctaca
    19141 cggcctggac gctcgcgttt atggctcggg aggctgtccc cgctcagcag aatgggacga
    19201 tgcctcggac gatcctcctg agtgcttcga gcgaattact ggcgatcggg cgcgcgccga
    19261 gctcgtgcgc tccctgctgc agagtcctct cctgggtgtg aaggccaaag tcaagcactt
    19321 gcccttgatg acgggcttgc tgctcgagcc tcgatacgcc tccagcctcg taggccccac
    19381 cagccgcgcg attgtagaag accaattcac gcgcgtgcgc gacggcgacc gcttctacta
    19441 ccgcaatcag atggatgctg atgaggtggc ctgggtggag gccttccgca tggcagatgt
    19501 ggttcgtgcc agcatcggcg ctgatgttgg tgtgcaggac gacgtgtttc acgtccctcc
    19561 gccggccttt ttcggtcgtg gacagtgacc cgtgcagcag ccgcgtcgcc gccccacatg
    19621 cagggcaagc ctgcgactgc ccgcggcaga atccgggagt acgtcggtct ttgagcctcg
    19681 gagcctaaca catgcaactt gcgccgctca tttgcgttca cgatgtcccc gccgctagcg
    19741 cttggtatcg atatttgctt ggctgcacga gcgggcatgg cggcgacgag tatgagcgcc
    19801 tgatggccga cgaacgactc atcttgcaat tgcatcgatg ggatgtcgag cacgagcacg
    19861 gcccgctcgg agatccagac gttcgtccgt acggcaacgg cgtcatcctg tggttcgagc
    19921 tgaacgattt tgaagatgcg gtgagtcgcg cccgggaaat gaaagtggaa actctgaagg
    19981 agcgtcacct gagcgaaaac ggcaactggg agtattggtt gcgcgaccca accggctaca
    20041 ccgttgtgct caccagtccg ctgccataga ccgagacatc gcactcagcc gaaggcaaag
    20101 tcccattcgg gctctcaaat ctccacgccc tccaatcgcc aagcttgcgc gagctgatcg
    20161 cgacacccct gctcgatgat gtcgccgtgg cccatgatga ggcgctcgca gggccgctgc
    20221 atcatggcct ttagtgagcg cgccgccgcc gccttgtccg ctagcttccg tctggcatcg
    20281 ggagggacgc ggacgcgttg gtagcagccg gtgagacgcc cggcgagccg ccacgtgaag
    20341 tggtccccaa cgcccgccga gcacacgagg tccgcgccaa tcagggttcg agtcggtcga
    20401 tggtagagca ctgtctcgtc caagaagggc acgccggcga ggggtagcgc ttcgaactcg
    20461 ggaatcaact tgccgaagtt gcccccgtgg aggtccacat cgagtctgag catcgcgttc
    20521 cttttgagcg cggaggccgg tccggcgagc ttcccttccg ggaagcgtgc cgccgccgcg
    20581 ggggctccca ggtgatgcca acagtttggg acgatgagcc agcgcaccgg acccaatgcg
    20641 cggagttcgt gaatcaaggc atcggtcggc gggggtggag tgtgaagcag caagctgccg
    20701 tcctcgagac gcacgacggt cgtgcgcgca cgcagccgaa ccccgccgaa ccacaccggc
    20761 cgttcgaggc tccagatcct gtcggcaacc actgcggtga gcgcgctgtc gatcatgggt
    20821 accgcgccgt ggcagctcgt ccgccccgcg agactgtaaa gtggactggc atgaagagtt
    20881 cgtatcagaa gatccgcggt cgaggccagg gaaccacatg tgcaaatttg cacatctgat
    20941 ttctatggat gtgcgtggac gaagaggaag ggcgggctcc gcgagcgggc tttccatgga
    21001 ctactaccgg agcaggcgtc gggcctcggc ttcgatttcc ctcgcgacga cgcggacacg
    21061 gggcgtcttt ctggcatcgc gacgaaacgc caggaacacc ggcaccggcg gcggcgtggt
    21121 ttcgaggtcg agttttacca agccggcgag cgagctcgct tgcacgacgc cgagcacgcc
    21181 cattcccata ccagccacga tcgcctgttc gatcgcgatg atggagttcg agcggaaggt
    21241 gaagcggctc gcgccgtacg cacgcatcca ctgctcgtgc ggcaagcgct cgaacgaggc
    21301 atcgagccct acccagtcgt gcagccctgc tccgtcccga gagagcctcg cgttcgacag
    21361 gcgccgctcg acgtactcgc gagaggcgaa cagcccagtg ctggcgcgtc caagtaactt
    21421 ggagactatc ggtgcggacg cggagcgcac gatacgaatg ccgatatcgg cctctccgcg
    21481 agccagatct gcgatccggg actcggagat gacctccacg cggagcgccg ggtgtttcgc
    21541 ttggatgcgc gcgagcaacg gcacaagggc acgcacgaag ccttcgctga gcgatacgcg
    21601 caccgtgccg gagacctcgg cttcacgccc gtcacggacc agggcgccca gccgtgactc
    21661 gagctcctcg gccaacgcca ctagccggag tgcgtcgatg tcaagttgag tgccgtcatt
    21721 accgcggtgc acgagagcgc gaccgatgct ccgttcaacg gcctccactc gccgcgcgac
    21781 cgtcgaggcg gcgactccca gcgattttcc cgcggcgaag aagctctttt cgcgatggac
    21841 cgccagcaaa aagcgaagat cgtcccacga cgcaggcttg gtcttcatag tcgagagcga
    21901 ttgacctcga gagcgattaa cagagtcggg tgacgcactg ccatttcaat tctccggatt
    21961 tctcgtcgga aacgcagtcg cagtccgtgc cgcagtggtc gccccggcaa tgctgctcct
    22021 ccgaagtgca ggcttttccg taaagttcgc cacaactctg agcgtccagg tccgctggca
    22081 tacagtctct ggtccgatcg tcgatgcgcc ccgaagcaca gggctcgagc atgccttcgg
    22141 cgtcgcacgt gcgggccgtg cgaaggccgg tccccgtgtc acagcacaaa gattccggtc
    22201 cacggctgca agtgtctgcc gggcaggaac tcaccatggt gtatccgctc gggcagctca
    22261 ccttaccatc gtggcatgtt gcgctcacgt ccgtgctggg cggctcagtg caagccgtcg
    22321 cgcagcgcac gttcccgaag tcttcaggcc ggcagtcgct cgtaccaccc gctccggccg
    22381 cggtcgtgtc gctcgctccg ccagcgcctc cggacgcatt cgccggggag tcatgatttt
    22441 cgtcttctgc gcaagcagcg agtagcgcta gccccgtgac gagggcgagc gcgagcgagc
    22501 cagacagcga atcaagacct cgaactcggt acccaccaaa accgctcatc aggtgactat
    22561 tcgcggagtc gtcgtgctcg tcaagatggg ttcgggggtt ctgggcggcc cgttgaaaag
    22621 ggcgcgaatc gcgtcggcgt gtcgcttcgg cgcaggcaaa cctacgagcg tggcggcgat
    22681 ccaccgcgca tgccgcccgg aataagccga accgcagcgc ctcagacgcc gcttgggatc
    22741 gacgagcgta ccggaaccgc gttacccttc gttaccactc gagcaggaac gtcggctgca
    22801 gttcggttcg gggataaagc ccgcatcgac actgagtaac gttgatacgc gcgcccgctc
    22861 cgaatactct acctctccat gaagtcggga cgcgcggggc agtcgagacg aatgacagac
    22921 tcgctagctg ctgttgttgt ggtgttcgtt gcgtccggct gcgggtccac cgaagagagc
    22981 gcgtccgctt cgggcggcgc cggagccacg agcaccagcg gtgccgctgg cactgtggtg
    23041 agctctcg
//
LOCUS       DASTKU010001087         5918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_660920, whole genome shotgun sequence.
ACCESSION   DASTKU010001087 DASTKU010000000
VERSION     DASTKU010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660920"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..802
                     /locus_tag="VFQ61_38870"
     CDS             305..802
                     /locus_tag="VFQ61_38870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960517.1"
                     /translation="MTPPLGAWGYTNPTRDAGLGVSLGGLGAGLITKQGLWFEGTFRL
                     LRGLETVGSDATALAGYAVGREVAPGNWDFSIPLYGGYRFANRPGASPSDGYPLTEHV
                     HFLVAGARAVWTRHYRTMALELSLGALAGLPISATPPASAYYSDPPTRSWVEGTALVG
                     WVFDG"
     gene            972..2783
                     /locus_tag="VFQ61_38875"
     CDS             972..2783
                     /locus_tag="VFQ61_38875"
                     /inference="COORDINATES: protein motif:HMM:NF013276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HET9960518.1"
                     /translation="MQKRAPLYGHVTLALSLSVWGCSSTDTPGLGAAGSANMPSAGGS
                     AGSSGVGGVSSASGGLVSVAGAIATGGGATNGGGTSTQVAGTSAGGGDTAQSGGRNSG
                     GAGAGGANTNGGNPTRGGSSNASGNDAGGRSSGGAPSTSGGRTNGGTSSLGGSSAGGA
                     SAGSTASGGAPPQTGLPSGVRALFPANGAQGSCPDPVLRITFESAPTLGNSGKIQVFN
                     ASDNSVASVDMAASTVTDTLGGTQISLQRTVYLEGNTVAIYLRNRALPYGQSYYVTVD
                     AGAIKPAGGGSFSLSGNTAWRFSTAAAAPSSFSQVSVALDGTGQFCSVQGAFDAFPNN
                     NQTPSVINVKRGTYHEVIHFRGKNNVTLRGEDRRGTRIEGVNNNNMNGGTATRALFGV
                     DASTSFAVENLTIQNLTPQGGSQAEALRMQNCDKCMVRDADILSLQDTLLWSGRIYAK
                     NCYIAGNVDYIWGTGAVYFDQCEIHTLGRKGYNVQSRNAASGYGYVFVDSKLTADAGI
                     TGDVLARIDVSEYPGSHVAYINCTLGSHIAPSGWVITGGSPGSSLRFWEYQSKDASGN
                     LVNTSQRAAGSTQISASQAAMMRDPSVVLGGWKPPTQ"
     gene            2908..4632
                     /locus_tag="VFQ61_38880"
     CDS             2908..4632
                     /locus_tag="VFQ61_38880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012232813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960519.1"
                     /translation="MRPMLFARTGLALSHPLDRLISALRKPSLAGCILVAMTGACASH
                     APDAGAIAEEAERKPTVGLEQQVALVSRTSALTATDRLAACATDPRVIAGLVSQRVCA
                     GADIFFRETFDGNGRTCGSCHPVANNFTLDKPFTDALLAANPADPLFVFQNNPALATL
                     ESSDDFLQKTGVRENLDGFSDLNNRFTVRAVPHIFSLAITTRPDPADDRVLLPGEVRP
                     TEATGWSGDGSPNDGSLRQFLTGAIKQHFPKDLSRTAGVSFRQPTELELDLTLEYQRS
                     VGRTNELDFTQLNLADSKANEGRLAYLDPLRARCNVCHSNGGANFIDTGLNRNFDTFA
                     RTVPDLLSFAGVTTDGGFGGQSLSTPNIDTLNVGTLGLTGFGDGTFNTPPVIEAVDTM
                     PCFHNNGFGPDIEGCIAFYASIFLPSPAAQKLDARFGAPVNITGDDIVNIGRFLRVLN
                     AAFNLDLAKQRLQAAQTLANRFHDAPLARSIQLQLLRLADKEISDALVIDLQNPPQLA
                     PINPPLHPIAQERLVSARDEISAALSAGTWSARTNRIGNALSRVLNARDQFGSNINFR
                     MGSGNIMF"
     gene            complement(5693..>5918)
                     /locus_tag="VFQ61_38885"
     CDS             complement(5693..>5918)
                     /locus_tag="VFQ61_38885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960520.1"
                     /translation="ESYRAIAPKKLAAALPAAPSNPEQTSRAAHTKRPSAKPSPRRQA
                     SAADPPETASKTRPAKSAPRKKRKSTKSSS"
BASE COUNT         1079 a         1778 c         1930 g         1131 t
ORIGIN      
        1 tcaatctact cgatggtcaa tccagcgggt gaccaggcgc ccgtgcccta ccagcggggt
       61 ccgaaagaaa gggtcgagct gcgggggccg ggaagcaggc cggagagtgt gcgcgctgac
      121 tggcgtgcaa cttgctcctg agaccagcat gcacggcctc ttcacctgtt ctcgattggt
      181 ggctgcgatc gttctgtgtg ggttcggtgt gcgtcccgct tttgcacagt cgctcgcggc
      241 gaccagtgtt gtgcaaacgg cacaggagcc gtcgagaaca cccccctatt ccatcgcggt
      301 cagattgacg ccaccacttg gcgcgtgggg ttacacgaat cccacgcgtg acgcgggctt
      361 gggcgtgagt ctgggcggtt tgggcgctgg cctgatcacg aagcaagggc tgtggttcga
      421 gggaacattt cgcctgctgc gtggcctcga gacggtgggc tccgacgcga cggccctggc
      481 aggctatgca gtcgggcgcg aggtcgcgcc agggaactgg gacttttcga ttcccttgta
      541 cggaggttat cgttttgcga atcgtcccgg ggcgagccct tccgacggct accccttgac
      601 cgaacacgtt catttcttgg tggcgggagc acgggcggtt tggacgaggc actatcgaac
      661 gatggcgctg gagttgagcc ttggtgcgct tgcggggctt ccgatttccg ccactccacc
      721 ggccagtgcc tactattcgg atccaccgac tcgctcttgg gtcgagggta cggcgctggt
      781 gggctgggta ttcgacggct gagcggccgc gcaaacgcca ggcttgggcg ggtaaggctc
      841 acgactgcgc tccggcccgc gtgtacgatt cccgctccag gaaaacctct gccgcgagcg
      901 acccatcttc gcgctctcgt ttcactgcac gaacaggcga gtacgtgcgc ccgagaacga
      961 gaagaacaga gatgcaaaaa cgagcaccgc tctacgggca tgtaacgctg gcgctgagtt
     1021 tgagtgtttg gggttgtagc tcgacggaca cgccgggcct cggcgccgcc ggatcggcga
     1081 acatgcccag tgctggaggt tccgccggtt cgagcggggt cggcggcgtt tcgagtgctt
     1141 cgggtgggct ggtctccgtc gctggtgcga tcgctacggg aggcggtgcc acgaacggag
     1201 gcggcacgag cactcaagtt gcaggaacat ctgcgggcgg tggtgacacc gcacagagtg
     1261 gcgggcgcaa ctcgggcgga gcgggcgcgg gtggagcgaa cacgaatggc gggaatccca
     1321 cgcggggcgg ctcgagtaac gcctcaggaa atgatgccgg cggacgatcg agcggcggtg
     1381 cgcccagcac gagtggagga agaacgaacg gcggaacctc gtcacttgga ggtagctccg
     1441 ccggtggagc cagcgcgggg tcgaccgcga gcggaggagc gccaccccag accggtttac
     1501 cgagcggcgt gcgagctctg ttcccggcca atggcgcgca gggcagctgt ccggatccgg
     1561 tgctgcggat caccttcgaa tccgcgccga cgctcgggaa ctcggggaag atccaggtct
     1621 tcaatgcctc ggacaacagc gtggcgagcg tggatatggc cgcgagtacg gtcaccgata
     1681 ctctgggtgg cacgcagata tcactgcagc gcaccgtata cctggagggt aatacggtcg
     1741 ccatctattt gcgaaaccgc gcgttgccgt atggtcagag ctattacgtc accgtcgacg
     1801 ccggtgccat caaacccgcg ggtggcggca gcttctctct ctccggcaat acggcctggc
     1861 gcttcagtac ggccgcggcc gcgccgagca gcttttcgca ggtgagtgtg gcgctcgacg
     1921 gaactggtca gttttgtagc gtgcagggcg ccttcgatgc ctttcccaac aacaaccaga
     1981 ccccgtccgt catcaacgtg aagcgcggaa cctatcacga ggtgatccat ttccgcggca
     2041 agaacaacgt gacgctgcgc ggggaagatc gcagaggcac gcgcatcgag ggtgtgaaca
     2101 acaacaacat gaacggaggg acggcgacgc gcgcgctgtt cggggtggac gcgagtacca
     2161 gctttgccgt cgagaacctc acgattcaga atttgacccc gcaagggggc tcgcaggcgg
     2221 aagcgctgcg catgcagaac tgcgacaagt gtatggtgcg ggacgcggat atattgagtc
     2281 tgcaggatac gctgctttgg agcggccgaa tctacgccaa gaattgctac atcgccggca
     2341 atgtcgacta catctggggc acgggcgcgg tgtacttcga tcaatgcgag atccacacgc
     2401 tgggccgcaa gggctacaac gtgcagtctc gcaatgcggc ctcaggctac ggctatgtct
     2461 tcgtcgactc gaaattgacc gctgacgcag ggatcaccgg tgacgtgttg gccagaatcg
     2521 acgtgagcga atatccgggc agccacgttg cgtatatcaa ctgtacttta ggtagccaca
     2581 tcgcgccctc gggctgggtc atcaccggcg gcagccccgg ttcgtcgctg cgcttttggg
     2641 aatatcagag taaagatgct tccggaaacc tagtgaacac cagtcagcgg gcagccgggt
     2701 ccacgcagat ctcggccagc caagccgcca tgatgcgcga tccgagcgtg gtgctcggcg
     2761 gttggaaacc gccaacccaa taaacgaaat tagtgcgagc cgcggtgaag gttccggtgg
     2821 gtgccgactc ctataggggt cgcggatagg catttcactc gttagcgacc gctcacggtc
     2881 aacctccacg ctctactcac gaaaggcatg cgtccgatgc tcttcgctcg cactggcttg
     2941 gctttgtcac accctctcga tcgtttgatt tcggctttgc gcaagccctc actggccggc
     3001 tgcatcttgg ttgcaatgac cggcgcgtgc gccagtcatg ccccggatgc cggcgcgatc
     3061 gccgaagaag ccgaacggaa gccgacggtc ggcttggagc agcaggttgc gctcgttagt
     3121 cgaacatcgg cgttgaccgc aaccgatcgt ttggcggcgt gtgcgactga cccgcgtgta
     3181 atagcgggct tggtctccca gcgcgtgtgc gcgggggcgg acatcttctt tcgcgagacg
     3241 ttcgacggca atgggcggac gtgcgggagt tgccaccccg tcgccaacaa ctttaccttg
     3301 gacaagccgt tcaccgacgc gcttctggcc gcaaatcccg ccgatccatt gttcgtcttc
     3361 cagaacaatc ccgcgctcgc aacactcgag tccagcgatg acttcctgca gaagacaggc
     3421 gtccgcgaga acttggatgg attctcggac ttgaataacc ggttcacggt gcgggccgtg
     3481 cctcacattt tttctttggc gatcacgact cgcccggatc cagcggatga ccgggttctg
     3541 ttgccgggcg aggtgcgacc caccgaagct acgggctggt cgggagacgg ctccccgaac
     3601 gacgggtcgc tccgtcaatt cttgacgggt gccatcaagc agcacttccc caaggacttg
     3661 agtcgaaccg ccggcgtatc cttccgccaa ccgacggagc tcgagctcga cctcacgctg
     3721 gagtatcagc gcagcgtggg gcggaccaac gagctcgact tcacgcagtt gaacttggcg
     3781 gatagcaagg cgaacgaggg ccggctcgct tatctcgacc ccctccgcgc gcgttgcaac
     3841 gtgtgtcact ccaacggcgg cgcgaacttc atagacacgg gcttgaatcg caacttcgat
     3901 acctttgcgc ggaccgttcc cgacctgctg agtttcgccg gagtgacgac ggacggcggt
     3961 tttggtggcc aaagcctgag cacccccaac atcgatactt tgaacgtggg aacgctgggg
     4021 ctgactgggt tcggtgacgg cacgttcaac acgccgcccg tcatcgaagc cgtagatacc
     4081 atgccttgct ttcacaacaa cggctttggc ccggacatcg agggctgcat cgccttctac
     4141 gccagcatct tcttgccgtc cccggcggct cagaagttgg atgcgcggtt tggcgcgccg
     4201 gtcaatatta ccggtgacga catcgtcaat atcggccggt tcttgcgcgt actgaacgcg
     4261 gccttcaacc tcgatctcgc caagcaacgc ttgcaagccg cgcaaacgct ggcgaaccgt
     4321 ttccacgacg cgcccctggc ccgttcgatc caactgcagc tgttgcgtct cgccgataaa
     4381 gagatctccg atgctctggt gatcgatctg caaaacccgc cccagctcgc gccaatcaat
     4441 ccgcccttgc acccgatcgc ccaagagcgc ttggtcagtg cccgagacga aatcagcgcc
     4501 gccctgagcg cgggcacatg gtccgctcgg accaatcgca tcggcaacgc gctctcccgg
     4561 gtgctgaacg cccgtgacca attcgggtcc aacatcaact tccgcatggg ctccggaaat
     4621 atcatgtttt gagttgaagg ccgttcggcg ggagagccct ttctcttccg ccgaacgctc
     4681 gtctgttttg tttggccaca ccgtcgacga gcgcgaccgc tagtccaatt tcgtcaactt
     4741 cgccgtctgc gggctcccaa tccgtcccaa cgccgcgccg gccctcaaac tccaatgtgg
     4801 tgacgccgcc agcacgtggg actcgccgcg ccaattcgcg gagcgctgca ccatgaacgg
     4861 tcgaggacgc gacttgccgt ttcaaggtgg acgcgcatgt gggggcctgc gcggcgttgc
     4921 gacggacagc gggttagcgg acggcgaagt cgaggacggg gacgagcggt cgcgctcgtc
     4981 tacagcttgg ccaaaacaaa ggcccggagt tgattcgata tcttccattg cgtcagctcc
     5041 tcgcgatccg agcgagttgc tctcagaagc attggacgcg gacgtggccg ttcgggcctc
     5101 catcgaatcc actgaggcag acggtgtttt ggacggtacg cggacaaatg cacgcgcagg
     5161 ccgctagctt ctcttcataa cagcggtcgt cggctagcca acgacacgcg gagtttggct
     5221 gttcgcgcaa taggaagccc gggacgcagg gcggtagtcc gtctggctcc ccagatgaac
     5281 tgtgcggaga cggggcggaa tttgcgggtg gtttatctgc gggaggagtg gaggagccgg
     5341 tggcgacgag tgcgggaggt gccgagttat cggtggagtc gcgctcgacc tcaccaccac
     5401 aggccaatag cattggcatc atcgcccccg ccgagatgag cgttccccag agtagggtgg
     5461 tattcgaaag tcttcgttca gactgacccc gagtgctcga agttgggaga atcgggaaga
     5521 gactgatcgt cggtccacaa ccatgcatgg agtcggtgta agcaactcgc gtgcctcgcc
     5581 gcgggtgcag tgagacgtgc ctacgtactc gcgtcggcga atcgactcgt gggggatcgt
     5641 cgccaactga gctcgtctca agcgctgaga cctgccaatc gagttggctt gctcacgagg
     5701 aggatttcgt ggatttgcgc tttttgcggg gagctgactt ggcagggcgc gtcttgctcg
     5761 ctgtttcggg cgggtctgct gccgaggctt gccgacgggg ggagggcttc gcggagggac
     5821 gcttggtgtg cgcggcgcga gatgtctgct cggggttgct gggcgcagcc ggaagcgctg
     5881 cggccagctt cttcggcgcg atcgctcgat agctctcg
//
LOCUS       DASTKU010001088        10005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_661215, whole genome shotgun sequence.
ACCESSION   DASTKU010001088 DASTKU010000000
VERSION     DASTKU010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661215"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFQ61_38890"
     CDS             complement(<1..537)
                     /locus_tag="VFQ61_38890"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9960521.1"
                     /translation="MNSNSSIAQSPAANGPFYRRHLAIAGCISALLGAGAWLNRSANS
                     QKAISESSHSHLSSQRDSTYQVTEVSPCHSDPASCVSLGSRILGREATRAAHLFHAAC
                     EAGHPLGCNDLGVLYRDGIGLQQDPVRAARYFEQACADDSAEGCNNLGVLKQRGLGTN
                     LDSERARALYRQACELGST"
     gene            complement(731..2065)
                     /locus_tag="VFQ61_38895"
     CDS             complement(731..2065)
                     /locus_tag="VFQ61_38895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9960522.1"
                     /translation="MSADSGVGRLRRLAASWIRSGIWIAAPPSLIAAYYLRQAHAAPS
                     AWLALGGAVLGSALLTWRSERALKRSLRGISNVLSAYSEGDFSIRARRQEAPIPLLDV
                     LNELNLLGDALRKHRLGELEAWTLLRKVLSEIDVVVLAFDTHGHVKLVNEAAAKALAR
                     NATQLVQESAEALGLAELLSGVAPRTIKSFEALNGGTWELRRGAFRLSGTPHTLVVLS
                     DVSGALSEQESEAWRRLIRVMGHEINNSLAPIQSISENLVQTLQRTPREDDFEQDLEA
                     GLSIIARRAAGLGRFMGAYSKLMRLPRPVLGRVPVRAWVERIVGLEQRLRAEVLAGPD
                     AVLLGDADQLDQLLINLLKNAVEASLPSGGVRIHWSIADRMLRLCIDDDGPGVSNTAN
                     LFVPFFTTKPEGSGIGLVLARQITEAHGGQLTLSTRDTTGAQAVIKLPLARD"
     gene            complement(2062..3441)
                     /locus_tag="VFQ61_38900"
     CDS             complement(2062..3441)
                     /locus_tag="VFQ61_38900"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HET9960523.1"
                     /translation="MSTDKKLPRVLVADDQADIREALRLLLKGSGFSVAQASSPAGVC
                     AAVSEEQLDLALLDMNYARDTTSGGEGLDLITQVRALDPHLPIIVMTAWGSVAGAVEA
                     MKRGGSDYIEKPWQNQRLLSLIRTQVELSSRLRQTQRLEAQAVREHERALPELIAHSK
                     PMQKVKQLVERVAQSDANVLITGEHGTGKDVVARVIHAASLRAAHSFIPVNAGALADG
                     VFESELFGHVKGAFTDAKADRIGCFELADGGTLFLDEIGTMPASQQAKLLRVLQSGEY
                     QRVGSSKTRHADVRVLSATNLAIADEARRGGFREDLLYRLNTVEIHLPPLRERGEDIA
                     ALATHFLGIKASRYKKKLTGFTPEAVTALLAHTWPGNVRELGHVVERAVVLAEGNRVD
                     VTDLNLRAPSVPGLDIERMTLIDAEGYLIRKALERTSGNVVAAADQLGLSRSALYRRL
                     QTLGIRVQE"
     gene            complement(3456..5894)
                     /locus_tag="VFQ61_38905"
     CDS             complement(3456..5894)
                     /locus_tag="VFQ61_38905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HET9960524.1"
                     /translation="MTTLLADLSFAFRLFRRAKAFYAAVLLLLIGGVASTSAVCAIVG
                     TLIWKPLPFAQPEQLNMIWATREQDQRFKVSVPDFNDWKQASHSFSHMAALRDWAFLL
                     NRPNELPLSLYGLHVSGEFFETLGVQPLIGRLLSPADDLPDSEPVAVISEIAWAQLFS
                     RDPSWIGRTIRLDHKDYRLVGVVGDSFHFGTYWNRARIWTTFAASYPKYTTTLAVLRD
                     DHEVLVTARRRPGVSQNRAQAELEAVARRITAAHPGHEVGVRVLDLDTELRGQTRNSA
                     WVLLVAVGLVFLAVCSNVSSLLLSRIQTRRGELATRVALGASPERLVRQLMTETLTLF
                     LLAFPASALIAATFMRFFEAMLLDPQLIEPTQIPFDWSLLLICLSISGLAGVVCAALP
                     AFAVVDRDLAGAVKRSALGSSLGRAQKHVRGALVVAQVALAIALLSGGGLAAQAFYRV
                     LNAPLGFEPNRVILLHVLAANYPPGDLDRVARFYDEVLRRTARLPGVLSVAVANAIVH
                     VPFEAIQFEIMGGANANRRRALLQEHNRVTPGYFSTLGIPLLAGRNFSEEDRLGTRPV
                     IIVSESVEKRYFSEPLSPTLHPALGRSIRLYGEDVDRTIVGIVGNVRGQDTINQPRTD
                     GYLPFAQSPFQGMHLLLRTNGNPAAIYEAPRVIRSIDPNVAVTSGWWFSQVIGGIVAQ
                     RSLATGLLLGFAGAALLLCAVGIYGLASQLSLQRTREIGIRLALGSPPLSAAALLVRA
                     ALRVLMLGVPLGLLLALGVGQLIARNVLEAEAFDFKLFLGVSALVVFASGAACALPAL
                     RALRISPASALRYE"
     gene            complement(5891..6628)
                     /locus_tag="VFQ61_38910"
     CDS             complement(5891..6628)
                     /locus_tag="VFQ61_38910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9960525.1"
                     /translation="MNPTELPDRQTSEESVDPLIRLSGISKVFPGDSIETLALSDVQV
                     EIRAGEYVAIEGPSGSGKTTLLSILGLLERPSAGQYQLRGRSVAGLSARERAQIRNLE
                     IGFIFQTFNLIGDLTVWENVELPLLYRDLSRSERSARVAAALDRVQMLHRRQHLPSQL
                     SGGQQQRVAVARAVAGEPRIILADEPTGSLDSVSGDLVMNLLDEVHASGATLCIVTHS
                     REYARRAHRTIRLFDGQIVDESARARV"
     gene            6879..7898
                     /locus_tag="VFQ61_38915"
     CDS             6879..7898
                     /locus_tag="VFQ61_38915"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET9960526.1"
                     /translation="MLRPLVAALAIALLSLAAPASARSREPLNFRVDLGTGKVQRLTR
                     LSEPPYRPPWPPPPRDKRRSLATRNGTLIVFDEEPRRGIRKLRWQQTLETGPGWFPWT
                     LIQQNTVTSLWSEEVPGQPHQYREVIRAIDLETREVLWTRVGPSHPDGGAEPLGPDRL
                     LVDQRNEVLLLETKTGKVLRSFRKDADFFSVSQPSPGRIWLEAGERIECLDELTADTL
                     WSVPKSGAVLSLRPIPKSEDWLVQTSSHVYRLRPADGLQAWSAVARGGSPPLLWNNRS
                     YEAWLEGSNPHTLMLVERDLQNGKVLREYVLGQHVGFFDKATVEAISLRAGAVYVVAD
                     FFVWE"
     gene            complement(8029..9450)
                     /locus_tag="VFQ61_38920"
     CDS             complement(8029..9450)
                     /locus_tag="VFQ61_38920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960527.1"
                     /translation="MLRNICKQSPRFIAMGTLCVAVSAGCTAPLAEDKAGVGSGSFSD
                     PVQIRRDNPAIGGRIYVDGTQVKGSVGSDTKSFSQAKWTNATGFLQVIEYRQNGTQYA
                     RYKTPLLPQEDFQSPGAAAFGTRAVPLVHGNVYAIGGQAAGSSIETQNAETWYAKLSD
                     DVAVVPIVLVHWVPRGGSSSSEFRATTAGAFDFYPAFAQYSGRPGVAGHAVPQKLQSP
                     TIDQQSVTSSNFNTWSAGLAQYRPFGRWTPPDELFAKCGVQFQVVDEVTITLPANWIA
                     GNPENCSVHGENAPNPRNAYLTAQNNSVLARYITEKLRPAFVSLANYQCSTWHGVTAN
                     GTGLVDLELNAPAGTLAHELGHVVGLSHTANTETTNLMHPGAGGPDLTDRQCQTARNV
                     AMNYSRRFDEFNRVTGRTYSSTPPDLAGEVEVDDGGGDFGGSGPVDLGICCSAFPVNT
                     AVSREQCTAAGGHEVECPQPDIR"
BASE COUNT         1905 a         3113 c         3183 g         1804 t
ORIGIN      
        1 cgtagaaccg agttcgcaag cctgtcggta gagagcgcgc gcccgctccg aatcgaggtt
       61 ggtcccgagc ccgcgttgtt ttagaacgcc aaggttattg cagccttctg cagagtcgtc
      121 agcacaagct tgctcgaagt agcgggctgc gcgcacaggg tcttgctgca gaccgatacc
      181 gtcccggtac agcaccccca agtcgttgca cccgagtgga tgacccgcct cacaagcggc
      241 atgaaagaga tgcgccgcgc gggtcgcctc acggcctagg atccgcgaac ccagcgagac
      301 gcaactcgcg gggtccgagt ggcacgggct gacttccgtc acctggtagg tcgaatcgcg
      361 ctgagaggag aggtgtgagt ggctggactc agatatggcc ttctggctat tggcactccg
      421 attgagccac gccccagcac cgagcagagc cgatatgcat ccagcaatgg cgagatgccg
      481 tcgatagaag ggaccgtttg cggcgggaga ctgtgcgatt gacgagttgc tgttcattgg
      541 attcccaatg cagcacccaa gagcacacgc cgtgccggcg cgaatattcg agcgaaccag
      601 caggaaatca ggacttgctt ggaagaacgt cccgcgctcg ggattgggcg atcccaagat
      661 tgggattcgc ggcgtgatcg ctcgcggaac gcgtcgggaa gctcgaagcg tcttgcgagc
      721 agtggtgtcg tcagtctctc gccaatggca gcttgatcac tgcttgcgcg ccggtcgtat
      781 ctcgcgtcga aagcgtgagt tgcccgccat gggcttcggt gatttggcga gccaacacca
      841 gcccgatccc ggaaccttcg ggcttggtcg tgaagaacgg cacgaaaaga ttggccgtgt
      901 tgctgactcc cggcccgtcg tcatcgatgc acaagcgcag catgcggtca gcgatggacc
      961 aatggatgcg caccccgccg ctgggcaggc tcgcttcgac ggcgttcttg agcaggttga
     1021 tcaagagctg atccagctga tcggcgtcgc cgagcagcac cgcgtccggt cccgcgagca
     1081 cttcggcgcg caaccgttgc tcgagcccaa cgatgcgctc cacccaagcc cgcacgggca
     1141 ctcggcccag taccggccgt ggcaagcgca tgagcttgga gtaggcaccc atgaagcgac
     1201 ccagcccggc ggcgcgacgc gcgatgatcg agaggcccgc ttccagatct tgctcgaagt
     1261 catcttcccg cggtgtgcgc tgcagcgttt gcacgagatt ctccgaaatc gattggatgg
     1321 gcgcgagcga gttgtttatc tcgtgaccca tgacgcggat caggcgtctc caagcttcgc
     1381 tttcttgctc gctcaacgcg ccgctcacgt ccgagagcac gaccaaggtg tgcggcgtgc
     1441 ctgaaagtct gaaggcgccg cgtcgaagct cccaggtccc cccgttcaac gcctcgaagg
     1501 atttgatggt gcggggggcg accccactca agagctcggc aaggcccaac gcctctgccg
     1561 actcctgcac gagctgggtg gcgttgcgtg ctagcgcttt ggccgcggct tcgttcacta
     1621 gtttgacgtg gccgtgggtg tcgaacgcga gcaccacgac atcgatttcg gagaggactt
     1681 tacggagcag ggtccaggct tcgagctcgc cgagacggtg tttccgtagc gcgtccccca
     1741 gcagattcag ctcgttcaga acatcgagca aggggatcgg tgcctcctgg cggcgagcac
     1801 ggatcgagaa atcaccttcg gagtaggccg agagcacgtt cgagatcccg cgcaggctgc
     1861 gcttcagggc tcgctcggaa cgccaagtga gcaatgccga gccgagcact gctccgccca
     1921 gtgcgagcca agccgagggc gccgcgtgcg cttgacgcag gtagtacgcg gcgatcaagc
     1981 tgggcggcgc cgcgatccag atgccgctac gaatccagct cgcggcgagc cgccgaagcc
     2041 ggcctacgcc ggagtccgcg ctcattcctg gactcgaata ccgagcgtct ggagccgtcg
     2101 gtagagggcg ctgcgagaga gtcccagttg atcggcggct gccaccacat tgccggacgt
     2161 acgctccaag gcctttcgga ttaggtatcc ttcggcgtcg atcaacgtca tgcgctcgat
     2221 gtccaggccg ggcacgctgg gcgcccgcaa gttcagatcc gtgacgtcca ctcggttgcc
     2281 ctccgcgagt acgacggcgc gctccacgac gtggccgagc tcccgaacgt tgccgggcca
     2341 agtgtgagct agcagtgcgg tcactgcttc gggcgtgaag cccgtcagct tcttcttgta
     2401 acgcgaggcc ttgattccga gaaagtgcgt cgcaagggca gcgatatctt caccgcgttc
     2461 acgcagggga ggcaggtgga tctcgaccgt attgagtcga tagagcaggt cctcgcggaa
     2521 cccgccacgc cgcgcctcat ccgcgatggc gaggttggtg gccgagagca cgcgcacatc
     2581 tgcgtggcgg gtcttggacg agccgacacg ctgatactcc ccactctgca ggacgcgcag
     2641 aagcttggct tgttgcgaag cgggcatcgt gccgatctcg tccaaaaaca aggtgccacc
     2701 atcggccagc tcgaagcaac caatgcgatc tgcctttgcg tcggtgaacg cgccttttac
     2761 atgcccgaat agctcgctct cgaagacgcc gtcggcgagg gcgccggcat tcacgggaat
     2821 gaagctgtgc gccgctcgca gcgatgcggc gtgaatgacg cgcgcgacca cgtctttgcc
     2881 ggtgccgtgt tcacccgtaa tcaacacgtt ggcgtcggac tgggcgacgc gttccaccag
     2941 ctgcttgacc ttctgcatgg gtttggagtg cgcgatcagc tctggcaagg cacgttcgtg
     3001 ctcgcgaacg gcctgggcct ccagacgctg agtctgccgg aggcgagacg agagctcgac
     3061 ttgcgtgcgg atcaagctga gcaagcgctg attttgccag ggcttctcga tgtaatcgga
     3121 tccgccacgt ttcatcgcct ccacggcccc ggccacgctg ccccaggccg tcatcacgat
     3181 gatgggcagg tgggggtcga gagcacggac ttgcgtgatc aggtcgagcc cctcgccgcc
     3241 ggaggtggtg tcccgagcgt agttcatgtc gagcagggcg aggtccagtt gctcttcgct
     3301 gaccgccgcg catacaccgg cgggagagga ggcttgggca acggagaagc cagagccctt
     3361 caggagtaag cgcaatgcct cgcggatgtc cgcttggtcg tctgcaacca gaactcgcgg
     3421 caacttcttg tcggtactca tcggcgtctc cgagtctact cgtagcgcag ggccgaggcc
     3481 ggggagatgc gcaacgcacg cagcgcaggg agggcgcagg cggcgccgct cgcaaagacc
     3541 accagcgccg ataccccgag aaacagcttg aagtcgaagg cctcggcctc gagaacattg
     3601 cgcgcgatca gctgacccac tcccagcgcg agcagcagac ccaggggcac accgagcatc
     3661 agaacccgca aggcggcgcg cacgagcagg gcggcggcgc tcagcggcgg tgagccgagc
     3721 gcgagccgaa tcccgatttc gcgcgttctt tgcagcgaga gctgtgaggc cagtccgtag
     3781 atgcctaccg cacaaagcaa gagggcggcg ccggcgaacc cgagcaacag acccgtggcc
     3841 agcgagcgct gcgcgacaat cccgccgatg acctgactga accaccagcc gctggtcacg
     3901 gcgacgttcg ggtcgatgct tcgaatcacc cgcggggcct cataaattgc ggcgggattg
     3961 ccgttggttc gcaacagaag gtgcatgcct tggaacgggc tctgagcaaa cggcaagtag
     4021 ccgtccgtgc ggggctgatt tatcgtgtct tgacctcgaa cgttgccgac gatcccgacg
     4081 atggtccggt ccacgtcttc tccgtacaag cggatactgc gtcccaacgc cgggtgaagc
     4141 gtagggctga gcggctccga aaaataccgt ttctccacgg actcacttac gatgatgacg
     4201 ggccgagtgc cgagacgatc ctcttcactg aagttgcgac cagccaagag cggaataccc
     4261 aacgtggaga agtagcccgg cgtgacgcgg ttgtgctctt ggagtagcgc gcgacgtcgg
     4321 ttcgcgttcg cgccgcccat gatctcgaat tgaatggcct cgaatgggac atgcacgatg
     4381 gcgttggcga cggccacgga caacacgccc ggaagccgcg cggtgcggcg caagacctca
     4441 tcatagaatc gggccactcg gtccagatcg ccaggaggat aattggccgc cagcacgtga
     4501 aggaggatga cgcggttggg ctcgaagccc aacggtgcgt tgagcacacg atagaaggct
     4561 tgggccgcca gtccaccccc gctcagcagt gcaatcgcca acgccacttg cgcgaccacg
     4621 agcgcgcccc gaacatgctt ctgtgctcga cctagcgagc tgccgagcgc actgcgcttc
     4681 actgctcctg ccaaatcgcg atccaccact gcaaaagcag gtaacgcggc gcataccacg
     4741 cccgccaacc ccgatatcga gagacaaatc agcagcagac tccagtcgaa tgggatctgc
     4801 gtgggctcga tgagctgggg atccagcagc atggcctcga agaaccgcat gaacgtcgcc
     4861 gcgatcaggg ccgaggcggg gaacgcgagc aagaacaagg tgagtgtctc cgtcatcagc
     4921 tgtcgcacga ggcgttccgg agaggcgccc aaagcaaccc gcgtggcgag ctccccacgc
     4981 cgggtctgaa tgcgggacaa gagcaggctg gagacgttgg agcagaccgc cagaaacacc
     5041 agtcccacgg ccaccagcaa gacccaggcg gagttccgcg tctggcctcg cagttcagtg
     5101 tccaagtcga gcacgcgcac gccgacttcg tgtccaggat gagccgcggt aatgcggcgg
     5161 gcgacggctt cgagttcagc ctgggcgcgg ttctgactga ctccgggcct tcgacgggcg
     5221 gtcacgagca cttcgtgatc gtcacgcagc accgctaggg tcgtcgtgta cttggggtag
     5281 gacgccgcga aggtggtcca gatccttgcc ctgttccaat aagttccgaa gtggaaagag
     5341 tcacccacca ccccgaccaa gcggtagtcc ttgtggtcga gacgaatcgt tcgaccaatc
     5401 cagctcggat ctcgcgaaaa cagctgggcc cacgcgatct cgctgatcac ggcgacgggt
     5461 tcgctgtccg gaagatcgtc ggcgggcgac aacaagcgtc cgatcagcgg ctgaacgccg
     5521 agcgtctcga agaactctcc cgagacgtgc agtccgtaaa gcgagagtgg gagttcattg
     5581 gggcgattca atagaaacgc ccaatctcgc aacgcggcca tgtgggaaaa gctgtggctg
     5641 gcctgcttcc aatcgttgaa gtcggggacg gagaccttga agcgctgatc ttgctcgcgc
     5701 gtcgcccaaa tcatgttgag ctgttccggt tgcgcgaatg gcagcggctt ccaaatcaga
     5761 gtcccgacga tcgcgcaaac ggcggaggtg gaggcgaccc cgccgatgag caaaagcagc
     5821 accgccgcgt aaaacgcctt ggccctgcgg aaaaggcgaa aggcgaaaga tagatccgcg
     5881 agcagcgtgg tcatacccgg gctcgcgctg actcatcgac gatttggcca tcgaacaggc
     5941 gaatcgtgcg gtgggcgcgc cgcgcatatt cgcggctgtg cgtgacgatg cagagcgtgg
     6001 cgcccgaggc gtgcacctca tccaacaggt tcatgaccaa atcaccgctc accgagtcga
     6061 ggctgcccgt cggctcgtct gccagaatga tgcgcggttc gccggctacg gctcgcgcga
     6121 cggccacgcg ttgctgctgc cctcctgaaa gctggctggg caggtgttgc cgccggtgga
     6181 gcatttgaac acgatccagg gcggccgcta cccgcgcgga gcgctcgctc cgggacaggt
     6241 cacggtacag tagaggcaat tcgacgtttt cccaaacggt gaggtcacct atcaaattga
     6301 aagtctgaaa gatgaacccg atttcgagat tccgaatttg ggctcgttca cgagccgaaa
     6361 gccccgctac cgacctacca cgcaactgat actgcccggc agagggccgc tcgagcagcc
     6421 ccaagatgga cagcagcgta gtcttgcccg aacccgaagg cccctcgata gcgacgtact
     6481 ccccggctcg gatctcgact tgtacgtccg agagcgcaag ggtttcgatc gagtcacccg
     6541 ggaaaacctt gctgatcccg gacagccgga tcaagggatc tacgctctct tccgaggttt
     6601 gccggtcggg gagttcggtc ggattcatgt cgctttcgtc gggctcgacg gtcggcggag
     6661 gggttcgagg ctggcgtcgg tcaccttcac gccttcgccg aatagcgaag gtcgtgccaa
     6721 acgtaaatca ccaaataccc gcgtaaagtg cggaacgatc gccaagtctt ctccgctttt
     6781 gggatccggg tgtcccggaa ttgggacgcc gccatctcga ggcacggcgc gtcgggaccg
     6841 tcgttcgggc tcgtatgggt gcgtgctaag ttcgaaccat gttgcgcccc ctggttgcgg
     6901 ccttggcgat cgcgctgctt tcgctcgccg cgccggcttc tgcgcgctcc cgcgagccgc
     6961 tcaacttccg ggtggacctc ggcacgggca aggtccagcg tcttacgcgc ctgagcgagc
     7021 cgccgtatag gcctccgtgg ccgcctccac ctagggacaa acgccgttcg ctcgccaccc
     7081 gcaatggcac actgatcgta ttcgacgaag agccgaggcg cggcatccgc aaactcagat
     7141 ggcagcagac cctcgaaacc ggtccgggtt ggtttccgtg gacgctcatc cagcagaaca
     7201 ctgtcacctc cctgtggagc gaagaagttc ctggtcagcc ccaccagtat cgcgaggtaa
     7261 tccgcgccat cgacctcgaa acccgcgagg tcttgtggac ccgcgtcggc cctagccacc
     7321 ctgacggtgg cgctgagcca ttgggtcccg atcgactgct cgtcgatcag cgcaacgaag
     7381 tgttgctact cgagaccaag acggggaagg tgctgcgttc tttccgcaaa gacgcagact
     7441 tcttttcggt ttcacagccg agtcccgggc gcatctggct ggaagcaggc gaacgcatcg
     7501 agtgtctcga cgagctgacc gccgacactc tgtggagcgt tcccaaatcc ggcgccgtcc
     7561 tctcactccg cccgattcca aaaagcgagg actggctggt gcagacctcg agccatgtct
     7621 accgcctgcg gcccgcggac gggctccagg cgtggtccgc cgtcgctcgt ggcggctcgc
     7681 ctccgctgct ctggaacaat cgcagttacg aagcatggct cgaaggcagc aatccgcaca
     7741 cgctgatgct ggtcgaacgt gaccttcaga atgggaaagt ccttcgcgaa tacgtgctgg
     7801 ggcagcacgt gggcttcttc gacaaggcaa ccgtcgaagc catctccctg cgcgctggcg
     7861 ccgtctacgt cgtcgcggac ttcttcgtgt gggaatgagg tgctgtcaac cgaagtgagg
     7921 tcgcgcttcc ggcattcagc accggttacc gaggatagcc cctgatcctc ggtaaccgat
     7981 ttccgaagcg cgcgggcgta ctccgacgtt gccgcagagc acgcgacctc agcgaatatc
     8041 cggctgtggg cattccactt cgtgcccacc ggcggccgta cattgctcac gcgagacggc
     8101 agtgttcacc gggaaggccg agcagcaaat tccgagatcc acaggccccg atccgccgaa
     8161 atcgccgccg ccgtcgtcga cttccacctc accggccaag tcgggcggag tgctgctgta
     8221 cgtgcggccc gtcacgcgat tgaattcgtc gaagcggcgc gagtagttca tggccacatt
     8281 gcgagccgtc tggcactgac gatccgtgag atcggggccg cccgcgccag gatgcatgag
     8341 gttcgttgtc tccgtattcg ccgtgtgcga cagccccact acgtgcccga gctcatgggc
     8401 gagtgttccg gcgggcgcat tgagctccaa atcgaccaac cccgttccat ttgcggtgac
     8461 gccgtgccac gtcgaacatt ggtagttcgc gaggctcacg aaggcgggcc tgagtttctc
     8521 ggtaatgtag cgagcgagca cgctattgtt ctgcgcggtc aagtatgcgt tgcgcggatt
     8581 gggcgcgttt tcaccgtgaa cgctgcagtt ctcgggattt cccgcgatcc agttcgcggg
     8641 tagagtgatc gtgacctcgt ctacgacctg aaactgaact ccgcacttgg cgaacagctc
     8701 atcggggggc gtccagcgtc cgaacgggcg gtactgcgct agacccgcgg accacgtgtt
     8761 gaagttcgat gaggtgaccg actgttgatc gatcgtcgga gactgcagct tctgaggtac
     8821 ggcgtggccc gcgacgccgg ggcggccgga gtattgggcg aaagccggat agaagtcgaa
     8881 agcgcccgcc gtggttgcgc ggaactccga agacgagctg ccgccgcgcg gtacccaatg
     8941 aacgagcacg atcggcacca ccgccacgtc atcggagagc ttggcatacc aggtctccgc
     9001 gttctgagtc tcgatgctgc tgcctgccgc ttgaccgccg atagcataga cattgccatg
     9061 cacgagcggc actgcccgcg tgccaaacgc ggctgctccg ggagattgga aatcttcctg
     9121 aggtagcaga ggtgtcttgt agcgcgcgta ctgggttccg ttctggcgat actctatgac
     9181 ttgcaggaag ccggtggcgt tggtccactt ggcttgcgag aaggacttgg tgtcggaccc
     9241 gacactaccc ttgacttgtg taccatccac gtagattctg ccgccgattg ccgggttatc
     9301 gcgtcgaatc tgcactggat cgctgaaaga gcccgaaccc actccggcct tgtcttctgc
     9361 caatggcgcg gtacagcctg ccgacacggc aacgcacagt gtacccatgg caatgaaacg
     9421 cggtgattgt ttgcagatgt tcctgagcat ttgatttcct gttctcgacg aggggggcag
     9481 ctttgggatc ctgggggcat cccgatcgag acgctctcgt tcggtgcgcg tcgcgcacgc
     9541 tactcccgac aagcgacctg tcgcttcgga gcccgccagt tcgcagaggc agttagcaag
     9601 gcgcgggcca acgctcgcgt agcccgaaac acgctcggaa tcaggggcga atcgagcttc
     9661 ctcggctctt gtggccggat tgggcaccag gggctctgtt ctcgtgtgcc agttctggca
     9721 ccagcgcgtg ccaggactga acgccgcgtc gccggggaca aaaggaagaa ggtcggagct
     9781 gccttgcgag gcaattccga ccttcggtgc tggcgcttct cagtggaccg cgggttcctc
     9841 tgccatcaac ccatgttccg tgtcaatgga ggccgattca cgttacttct gagcgggctg
     9901 ctgccaagtt gcgcagtgga tccctccgcc ataccagttg tagttgatca cgtgggcata
     9961 cggaaactga acgatgcgtc gctcggggta caagctccga aaggt
//
LOCUS       DASTKU010001089        18837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1556638, whole genome shotgun sequence.
ACCESSION   DASTKU010001089 DASTKU010000000
VERSION     DASTKU010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 18837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18837
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556638"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VFQ61_38925"
     CDS             complement(<1..1085)
                     /locus_tag="VFQ61_38925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960528.1"
                     /translation="MASAAAWLALSIGMLGFAGWLFDSPTLKTAFTGNVSMKANASLG
                     ICVLAFASLCLLPSKRSVHQLRMARAAGLFAILLGATTLAEHAFDWNAGIDELLFTEP
                     AGEAATASPGRVGPPASTSFVLLGIGLLCFDKRTSRGSSWSQRMALLTAPGALLGILG
                     YITHATQLYGIAKYTGIALHTAVAILALAVFQFLARAEYEPAALLVLDNAGGEVARTV
                     LPAAIVLPLVVGWLRTSGERLGLFDTAFGRALLLLTLMLLLTIVVWRVAKRLSGVAIQ
                     HAAAERASSHARAEAQRLAAEHVETLSVLESLLAHAPIGFVFFDRQERCIRVNQYLTQ
                     NLPSPGVSASVRELLPGFNEELHTAIHR"
     gene            1403..2416
                     /locus_tag="VFQ61_38930"
     CDS             1403..2416
                     /locus_tag="VFQ61_38930"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9960529.1"
                     /translation="MNSLLKTAGAVLGAAVLTKMVVNRAREARYPISGKVVLITGGSR
                     GLGLLLARELSAHGAKLVLLARDPSTLETACSELRSRGEVLGIPCDVTDPHQVNAAVA
                     RAQQVFGRIDLVINDAGRIEVGPARAMTTADYEAAMATNFWGAYHVIHAVLPQMRAQG
                     EGRIVNISSIGGLLSVPHLLPYSASKFALTGYSLGLRAELARENIVVTTVCPGLMRTG
                     SPINANFKGNQAAEYAWFTAADSLPGLSMSGERAARQIVRAARRGQALSVLSLPAQLG
                     AFLQGTAPNLMAELMTLTARGLPDADSPATRPGRMLRSPRLRWLTVLTETAARRNNQW
                     GQL"
     gene            complement(2474..3301)
                     /locus_tag="VFQ61_38935"
     CDS             complement(2474..3301)
                     /locus_tag="VFQ61_38935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960530.1"
                     /translation="MTPPAAPATSSDRSAEAVDSGASDGSGSVSAAPDELPPVSTDWC
                     TAPVQVLDQDTCYVLPEDEQGVVQPGTTSKRAQVRELLIYLHGVVPPGRVSPQKTHFQ
                     EVVANAARRARIAALIPRGKRGLAPKGFAGWWGWPTSAEAYKREGAQLAAAFEAKQRM
                     LESITGQPFERRYLGGSSAGAYFVTLLMADGAFPAQGYMALAGGAIPPGRNLRADPAS
                     VYVGYGKFDPVGSSSRALADFLSRAGWPVRVAVHPVPHGASEVYLDEAFAFFRSSGN"
     gene            3665..5545
                     /locus_tag="VFQ61_38940"
     CDS             3665..5545
                     /locus_tag="VFQ61_38940"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9960531.1"
                     /translation="MSDSLTPTVPLARSIRQLKPYFAEHRAGLLAVVVLAIGTASLSA
                     LEPLVLKGLFDRFLEVRGVGEVLVPFSSLLGIVLARELLLPIQDRLFWRSRLGVNFGL
                     LQATVDRLHSLPLEYHRDQSVGATMTKIERGISMAMAAFTEVLVQLFPALIYLCVSVV
                     VMVQLDFRLFMLSLLFAPLPALVGAFAAREQSQRERSLMQLWTRIFARFNEVLSGIVV
                     VKSFVMEEQEKRRFLNGVQNANAVVLRGVGTDAKVNAIKSAIMTCARLSALGLGGTLV
                     MRRQISVGTLIAFVAYLGGLFQSVQTLTGMYQTLRRASAAVSSILSILEAQDSLGDSK
                     DARDVTELSGDIEFRDVSFGYRPGLEVLRNVSLRVRPGEMVALVGPSGAGKSTMMALL
                     QRLYDPTSGSILVDGQDLKALKQRSLRFQIGVVLQEGMLFSDSIRDNIAFGRPRASAA
                     EIEAAARAANAHEFICRLPEGYETQVGERGSKLSGGERQRVAIARALLKNAPILILDE
                     ATSALDAESEAKVQAALTRLIAGRTTFVIAHRLSTITSADRILVLKDGGIAEMGNHRE
                     LMARGGYYASLVRQQSRGLLDGSGQSLASSDRRSHPPEPLRLGPSSRPSHRPVALDTN
                     TA"
     gene            complement(5509..7500)
                     /locus_tag="VFQ61_38945"
     CDS             complement(5509..7500)
                     /locus_tag="VFQ61_38945"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9960532.1"
                     /translation="MLRSSLWRWSSSCAMFCACNAPVRMQPTGAGERQSARTSELQLW
                     PASAELFTFALELRTKHDGPQAPLDCSFRVGSRALPATRGSVFYEAHARLQPGLNQVV
                     ASCRDAAGGVIASNPESYDVRLKPQPHAAIALTVRGGEIELSARQSAVNSESNSPLRE
                     YRWSQRTRLGGPFNTLGSGPAWTGSAPADGSSIYRLTVTDADGEVGSASVGIQVRDGQ
                     PWSCADREKRRELFAGSVLYGVLPPLFGAQPLRAVEQRLSKLAELGVDVLWLAPTFEN
                     KAGDFGYAVEDYFRVRSDYGTPGDLKSLIGSAHRLGMRVILDLVPNHTSSTHRYFHQG
                     MELGARSHYFGFYQRTPDGLSTHYFDWQHLPNLDYGNAEVRRFMSEAASYWLEDFDVD
                     GYRVDAAWGIREREPAFFAPWLRQLSTIQPCAWMLAEAPGRDAYYVEQGFDFAYDWGP
                     ELGHWAFEEVFTSQSGIAARFANVLQSALQAGKHDVHQVVRFLNNNDTGPRFITRYGL
                     PLTRVATVALFTLPGVPALYSFDEEGAEFEPYSKLTPIVAPPHPELYDFHRKLIALRH
                     AQRELQRGELELLAVDPKGEGLAFARVSDAQLAVVALNFGARPVVLRFAPVKPSGNAT
                     RKILRDALHPSRPALDLTRPQVYLGAHDYAVFVSSATGR"
     gene            7920..9692
                     /locus_tag="VFQ61_38950"
     CDS             7920..9692
                     /locus_tag="VFQ61_38950"
                     /inference="COORDINATES: protein motif:HMM:NF006129.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HET9960533.1"
                     /translation="MNVSDYVLERLVAWGVRRVFGYPGDGINGLMGAFNRRKQADLRF
                     IQARHEETAAFMACGHAKFTGEVGVCLATSGPGAIHLLNGLYDAKMDHVPVVALVGQT
                     ARSALGGDYQQEVDLKSLFKDVAHEYVHTCMTPESIRHLVDRAFRTASAERTVTCVIL
                     PKDVQELEFEPPPHAHDTIHSAPGFSRPRVIPRTTDIERAAEILNAAERVAMLVGNGA
                     RDATEQVVAVAERLSAGVAKALLGRTVVPDDLPFVTGSIGLIGTEPSWKLMQECDALL
                     MVGSSFPYSEFLPKEGKARGIQIDIDGRMLGIRYPMELNLVGDARETLELLLPHLRQK
                     EHGEFRRNILQWNADWEKLSEQRASTQANPINPQLVFREGSKYLPDRCIISADSGSAA
                     AWYARDLKLRSGMLASLSGSLATMGPAVPYAIAAKFAHPDRPAIAFVGDGAMQMNGNA
                     ELLTIARHWREWADPRLVVLVLHNNDLNFVTWEMRAMAGDARYEASQDLPEFPYAAYA
                     ELIGLRGIQVEQPNQVTDAWRQALQADRPVVMDVRVDPDVPPMPPHVTLKQAKAFAMS
                     LQQEGSPGGVIKQVIQQVFPKKPT"
     gene            complement(9853..10410)
                     /locus_tag="VFQ61_38955"
     CDS             complement(9853..10410)
                     /locus_tag="VFQ61_38955"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9960534.1"
                     /translation="MARMISNGIDVVTFLKEQHQQIKAGFKEVLATSGEPRRTAFYAL
                     RRLLAVHETAEEEIVHPAARKALSDGESVVQARLEEENAAKQVLSQLEDMDVDSMDFE
                     VQFTTLQASVIAHAEAEEMEEFDRLGNQMEPERLQKMRKAVTFAESIAPTRPHPGVES
                     AAANMLAGPFASMLDRARDALTGKA"
     gene            complement(10466..10618)
                     /locus_tag="VFQ61_38960"
     CDS             complement(10466..10618)
                     /locus_tag="VFQ61_38960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960535.1"
                     /translation="MPSERSVARHGQRGWCEIGRARGLQRIEDRLALVLVNWVTVLQE
                     LVLEAT"
     gene            10718..11464
                     /locus_tag="VFQ61_38965"
     CDS             10718..11464
                     /locus_tag="VFQ61_38965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960536.1"
                     /translation="MAASKKNVSGSARATIDHDEIREWVEAHGGHPAHVKSTARGKSS
                     GILRIDFPGFSGEGALEPVEWEEFFEHFENSQLAFLYQDKTASGRPSNFNKLVKRDSV
                     ELTPSRTRKSSSRKSTGARAQASARKATPRKATARKGAARKAASRSSSTRSSSSVKKT
                     SSRRGATGGSSTGRSASSGSSSGRASSRSKTGRKAGGQNQTGRRGAAGTRSGRSQSGK
                     TARAPSQRDAPKRVAKKAGGSRARKTSKKK"
     gene            11684..12430
                     /locus_tag="VFQ61_38970"
     CDS             11684..12430
                     /locus_tag="VFQ61_38970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960537.1"
                     /translation="MEDDELDEDLDDDDLDEELDEDSEDELDDDSDDEDFDDEDSDDE
                     DFEDPANLSADAADDEDAALRQSISDGYDADAGPLETGYASEAVPLSEILNNAALDDE
                     SLSLEPEDLGRHALQSAAQQGDPIASPARIALDEAEPNASPNPQALTEPSNVDLTQSA
                     IREGSLFDQAREGEETKLRHPLVNTNEVDAGLKLNERAQRAGTKTALPGRVASEQTAT
                     RGNVKKPGATTPKSAKTSSAKDGSARSRRS"
     gene            12402..13847
                     /locus_tag="VFQ61_38975"
     CDS             12402..13847
                     /locus_tag="VFQ61_38975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960538.1"
                     /translation="MDRRGLGEAEYALKTFNPSRYRSEPLDSARTSVEVVRRGQRFGS
                     FRFDPRRPSYYLVSLASCVGMLAWPTAALAEDGGNEVCPANYASANDVRERYDARGRL
                     VHQLRLEAGQQVEEIAIQYGGAHATERTELTPGRRRVAKTQWNGDSVTLAECYVDGVL
                     TASATYGYQDTRVALVTRRFYEKPATERVETTRFFYDYDGQLIGTELRDGEQRLLSTV
                     RAERAPLRVPVQLSFNAGGSYQTDTNLYDVTAGFGIHRRPQAHRYGADPLEVELDGNF
                     RFHRAAGITSTDQTTARFSADYHEILPHVTLFTFTASDRNLPANLRLNLEQALLGVKY
                     DFLPRTDYQLDISFAPIWNFRSIIAPPPAGEEGAPSVNETTSKLRGSLRARAGVHRTT
                     WALRDTFEFLPTLYGDDVPQENGFWNRAMVRNTVTLELNFTPYLSLREEFKYTRDPAM
                     RAQASCPDDSNPLCRGYSIVSTSSIVLRLDL"
     gene            complement(13876..15987)
                     /locus_tag="VFQ61_38980"
     CDS             complement(13876..15987)
                     /locus_tag="VFQ61_38980"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HET9960539.1"
                     /translation="MQKVPVWTAGCCLGLSVCGGEVKTEQAPSAIVEPLVTAKACAPP
                     SMPPDPLVEPLNGGPTVNVWPPHGARFFRGARFDIRVEGTGTAPNPRYAASLKIDGEL
                     RAFSSGAEDSNGTDGITTAGWGGFNLRGYSNTKAGVHELDATFSSSLGTKRIRSHFLI
                     EDLDAAPPGHRLKNVILLLGDGMGVGVRTAARVARFGVARGTTRGWLEMDRFPSTGLV
                     TTHSLNNYLTDSAPGFAAYSTGTHNNNNQEGVFPAQVVSPFFQPRVENMAEYLHRTQG
                     KISGIVTTADVEDATPGAMAVHTGDRNAGTGICDQFLDESDPNRSGVSGTGLRVLLGG
                     GRKWFLPQSAAGSARTAATDYPALPADLVAAWSLPPAAAGALDPQRDLIADFGTAGFT
                     YVADKAQLDAALAQEVPSMLLGLFHLANMNVAVDKIARRRGVPVDGATEFANQFTPPK
                     SPFAADNDGFPNQPMLDEMTDAALRVLSKHDKGFALLVEGASIDKQEHTQDSDRAIAD
                     AIEFDRAVGVARRFAETDGNTLVLVLADHECGGFVAMGGLRNPVTNTNIGSLSYLQAL
                     PSEAGVLSPDVQPERQKVVGTNFTNGGFPTYEILPDGYPENFNVDGKMLIGYGASADR
                     FERWLTRPLPGPDGLGAWDRAAGMFVRGQGVKSGNAPHGASDVPVSAYAPKEEAHRRF
                     VGTYENIDVYFRVMAVLGAGR"
     gene            complement(16094..16507)
                     /locus_tag="VFQ61_38985"
     CDS             complement(16094..16507)
                     /locus_tag="VFQ61_38985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960540.1"
                     /translation="MATLDFRELYRFGDRAPELTDRARDLAGKRGTLVGFMVLLENPV
                     HGGFYLSPYPAISDESGAGRGGVPPTAVLVLLSAAGTRPIDFVPGALEVSGTFELGTA
                     EKAGEISSVRLIVDDARGIRFARTRTTLKVPAPRG"
     gene            complement(17059..18012)
                     /locus_tag="VFQ61_38990"
     CDS             complement(17059..18012)
                     /locus_tag="VFQ61_38990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03885 family FMN-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET9960541.1"
                     /translation="MTIEFGYHASHEQFPPSALLEAVRHADGLGILHGMCSDHFSPFS
                     TAQGQSGFAWSWLGAALQATRMTFGTVTAPGQRYHPAIVAQGAATLTQMFPGRFWLAL
                     GSGENLNEHITGQHWPVKAQRQARLRECVEVMRALFAGETVTHAGLVTVERAKLYTRP
                     EVPPPLFGAAVSAETAAWAARWADGLITVNQPREQLARVVEAFRENGGAGKPMYLQVH
                     VALAKQRRAALEAAHEQWRTGALAGPLLWDVAVPEQLDEAARFVRPEDVESSVRVTND
                     VEELVGEFRADQNLGFQRIYVHEVGRDQQSTFRQLVGALTE"
     gene            18192..>18837
                     /locus_tag="VFQ61_38995"
     CDS             18192..>18837
                     /locus_tag="VFQ61_38995"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET9960542.1"
                     /translation="MIKDLWYKNAVIYCLHVGTFMDSNADGIGDFEGLVRRLDYLAGL
                     GVTCLWLHPFQKSPRRDDGYDISDFYTVDPVFGTLGDFVQFTHEAKQRGIRVIIDLVV
                     NHTSDQHPWFKAARKNKQSKYRDYYIWSAKRPKDADTGMVFPGVQKTTWTRDSESGEY
                     YFHRFYEFQPDLNTSNPAVQEEIRKIMGFWLQLGVAGFRMDAIPFVIQTKGAGIK"
BASE COUNT         3603 a         5806 c         5906 g         3522 t
ORIGIN      
        1 cgatgaatgg cggtgtgcaa ctcttcgttg aaaccgggca gcagttctct aaccgaagcg
       61 gacactccgg gggagggtag gttttgggtc agatactggt tgactcggat gcaacgctct
      121 tgccgatcga agaacacgaa cccgatgggc gcgtgtgcca agagcgactc gagcacgctc
      181 agcgtctcga cgtgttccgc cgccaaacgc tgggcttcgg ctcgtgcatg gctggaggcc
      241 cgctccgctg ccgcatgctg gatcgcgacg cccgagagcc tcttggcgac gcgccacacc
      301 acgatggtga gcagaagcat caacgtgagc agcaagagcg cccggccgaa ggcggtatcg
      361 aagagtccta gcctctctcc gctcgtgcgt aaccaaccca cgaccagggg caacacgatc
      421 gccgccggca atacggtccg ggcgacttca ccgcccgcgt tgtcgagcac cagcagcgcc
      481 gcgggctcgt actcggcgcg ggcgagaaac tgaaacaccg ccaacgcgag tatcgcgacc
      541 gccgtatgaa gggcgattcc ggtgtacttc gcaatcccgt aaagttgggt tgcgtgcgtg
      601 atatagccca ggatgccgag caaggcgccg ggtgcggtga gcagagccat gcgctgagac
      661 caggagcttc cgcgagacgt gcgtttgtcg aaacacagca ggccgatgcc caagagcacg
      721 aatgaggtgc tcgcaggggg gcccacgcgg ccggggctgg cggtcgccgc ctcgccggcc
      781 ggctcggtga agagcagctc gtcgatgcct gcattccaat cgaacgcgtg ttcggccagc
      841 gtggtcgccc cgagcagaat cgcgaacagt cctgcggcgc gggccatccg cagttgatga
      901 acggagcgct tgctcggtaa caggcacagc gacgcaaacg cgagtacgca aatgcccagc
      961 gaggcgttgg ccttcatgct gacgttgccc gtaaaagctg ttttcagcgt ggggctgtcg
     1021 aacaaccaac ctgcgaaccc cagcattcca atcgataggg cgagccaagc cgcagcactc
     1081 gccacgagcc gcgaacttct caaggcttgc tgtcggagtc gcatgaatgg cgcagtctaa
     1141 tgcattgcgg aagaactcaa tgcgcatggg tgctcatgcg ggactgattt caccggctgc
     1201 gtttcgccgt gcagcgcaag ctcgctcgcg ctgcggtcgg ctcggactcg ctccgtgacg
     1261 gcagcggcgt gagtcgcgtt ttgccacgga tgcgtgaggt ggcatgttcc gtgctctttg
     1321 cagtgcttac caaaggattg cggtgccttg gactgcgcca atcgggcgag ctccgagccc
     1381 gccgtgccac gacggagttg ccatgaattc tttgctcaag accgcgggag ccgtgcttgg
     1441 cgcggccgtt ctaaccaaaa tggtggtgaa ccgagcgcgg gaagcgcgtt atccgatttc
     1501 gggtaaggtg gtgttgatta ccgggggatc gcgcggtctg gggttgttat tggcgcgcga
     1561 attgagtgca catggggcaa agctggtgct cttggcgcgt gatccttcta ccttggaaac
     1621 ggcctgttcc gagctgagga gcaggggcga agtgctcggc atcccctgtg atgtgaccga
     1681 cccgcatcag gtgaacgccg ccgtggcgcg cgcacaacaa gtcttcggac gaatcgatct
     1741 cgtgatcaac gatgccgggc gcatcgaggt gggtccggct cgtgcgatga ccacggccga
     1801 ctacgaggcg gccatggcga ccaatttctg gggtgcctat cacgtgatcc acgcggtgct
     1861 gcctcagatg cgcgcgcaag gcgagggccg tatcgtgaac atctcgtcca tcggcgggct
     1921 gctcagcgtg ccgcacctgt tgccgtatag cgcgagcaag ttcgcgttga ccgggtactc
     1981 cttgggttta cgcgcggagc tcgcgcgcga aaacatcgtg gtcaccacgg tgtgccccgg
     2041 tttgatgagg acgggaagtc cgatcaacgc gaacttcaag ggcaatcaag ctgcggagta
     2101 tgcttggttc acggccgctg attctttgcc aggtctctcg atgtctgggg aacgtgctgc
     2161 gcgtcaaatc gtgcgcgcgg cgcgccgggg acaggccctt agcgtgttgt ctctcccggc
     2221 gcagctggga gcttttctgc agggcaccgc gcccaacctg atggcagagc taatgactct
     2281 gaccgcgcgc gggctgcccg acgccgattc gcctgcgacg cgcccgggcc ggatgttgag
     2341 gtcgccgcgc ttgcgctggt tgaccgtgct taccgagacg gctgcgcgca gaaacaacca
     2401 atggggccag ctttgaattg atctaccacg cgagcacgag cggggcttcg ccaagacaag
     2461 gctccaaacg gcttcagttg ccgctcgagc gaaagaacgc gaacgcctcg tccaggtaaa
     2521 cctcactggc cccgtgggga acgggatgta ccgccactct tacgggccat cccgcgcgac
     2581 tgaggaagtc cgcgagcgcc cgtgaactcg agcccacggg atcgaacttg ccgtacccca
     2641 cgtaaacaga ggccggatcc gcgcgcaagt tgcgacccgg cgggattgct ccccctgcaa
     2701 gagccatata accctgagcc ggaaatgcgc cgtcggccat cagcagagtt acgaagtagg
     2761 caccggcaga agagcccccc aagtaacgac gctcgaatgg ctgcccggtg atgctttcga
     2821 gcattctttg cttggcttcg aaagctgccg ccaattgagc gccttcacgc ttgtacgcct
     2881 cggcgctcgt tggccaaccc caccaaccgg caaaaccctt gggggctaaa ccacgtttac
     2941 cccgtgggat gagcgcggct atccgagctc gccgtgcggc attggcgacg acttcttgga
     3001 agtgtgtctt ttgtgggctc acccggcctg ggggcaccac gccatgcaaa tagatcaaga
     3061 gctcacggac ctgagctcgc ttgctcgtcg taccgggttg aaccacaccc tgctcgtctt
     3121 ccggcagcac gtagcaggta tcctgatcga gcacctgcac gggtgccgtg caccaatcgg
     3181 tgcttacggg aggcagctcg tcgggagctg cggagacaga gcccgagccg tcggacgctc
     3241 ccgaatctac agcttctgcg gatcggtccg aggaggtcgc gggcgctgcg ggaggggtca
     3301 atctttctcg gtctgcaccg ttctcactcg gctcaaccct ggatccgcct tgcgggcgcg
     3361 gggctgccgg ggaaacacgg gcgggcgcgg gtgcgcggtg ctcggaagct ggggaacccg
     3421 ctgagttcgg gacggcagca ggcgagcctg aacaagcgaa accgagcgcg ctgcagctga
     3481 gtgcggtcat cctgacgagt gtcagagcgg caggtcgggt gggagacacg agatgagaca
     3541 tgacgtggcg aactcatcgc cgcaacgggt tcaccttttg cggctccact ctgtgccgtt
     3601 attgagcggc aaaaccccga ctcggtcgcg cgcgcttgct cgcggcagcc atgtccgaca
     3661 gccaatgtcc gacagcctca cacccaccgt ccccctcgca cgttctatcc gccagctcaa
     3721 accctacttt gcggagcacc gcgcgggcct gctggcagtc gtggtgttgg cgatcggcac
     3781 ggcatcattg tccgcgctcg agccgctcgt cttgaagggg ctattcgatc gctttctgga
     3841 ggtgcgcggc gtcggcgaag tgctggttcc gttctcgagc ttgctcggca tcgtgctcgc
     3901 gcgcgagctg ttgctgccga tccaggaccg gctgttttgg cgttcccgct tgggcgtgaa
     3961 ctttggcttg ttgcaggcga ccgtcgatcg actgcattca ttgcccctcg aataccatcg
     4021 ggatcaaagt gtgggggcta ccatgaccaa aatcgagcgc ggcatctcga tggccatggc
     4081 ggcgttcacg gaggtgttgg tacagctctt cccggcgctg atctacttgt gtgtttccgt
     4141 cgtggtgatg gttcagctgg acttccggtt gttcatgctg tcgctgctgt ttgcgccgct
     4201 gccggcactg gtcggcgctt ttgcggcacg cgagcagagt caaagagagc gcagcttgat
     4261 gcagctctgg acccgaatct ttgcccggtt caacgaagta ctgagcggta tcgtggtcgt
     4321 caagagcttc gtgatggagg agcaagagaa gagacgcttc ttgaacgggg ttcaaaacgc
     4381 gaatgcggtc gtcctgcgcg gtgttggaac ggacgccaag gtgaacgcca tcaagagcgc
     4441 aatcatgacg tgcgctcggc tgtccgcgct cgggctgggc ggaacgctcg tgatgcgccg
     4501 acaaatcagc gtcggcaccc tgatcgcgtt cgtggcttac ctcggcgggt tgttccagtc
     4561 ggtccagacc ttgacgggta tgtatcagac gttgcgccgg gctagcgccg ccgtgtcttc
     4621 catcctctcg atattggagg cccaagactc gctgggggac tccaaagatg cacgcgatgt
     4681 gaccgagctg tcgggtgata tcgagtttcg cgacgtcagc tttggttatc gtcccggact
     4741 cgaggtgctt aggaacgtca gtctgcgcgt ccgtcccggt gagatggtcg ccttggtcgg
     4801 cccgagtggg gccggcaaga gcacgatgat ggcgttgttg cagcgccttt acgatcccac
     4861 ctcgggaagc atcctggtcg acgggcaaga tctcaaggca ctcaagcagc ggtcccttcg
     4921 cttccaaatc ggggtggtct tgcaagaggg aatgctgttt agcgatagca ttcgtgacaa
     4981 catcgcgttc ggccgccccc gcgccagcgc ggccgagatc gaggccgctg cacgcgcggc
     5041 caatgcccac gagttcatct gtcgcttacc agaggggtac gagactcaag tcggtgagcg
     5101 gggctccaaa ctctccggtg gcgagcgcca gcgcgtggcc atcgcgcgcg ccttgctcaa
     5161 gaatgcgccc attctaattc tggacgaggc gacctccgcg ctggatgccg agagtgaggc
     5221 caaggttcaa gcggctctga cgcgcctcat tgcgggccga accaccttcg tgattgctca
     5281 ccgtctgtcg accatcactt cggccgatcg gatcttggtg ctgaaggacg gcggaatcgc
     5341 cgaaatgggc aatcaccggg agttgatggc gcggggaggg tactatgcct cgctggtccg
     5401 ccaacagagc cgagggttgc tcgatggttc tggccaatcc cttgcgtcca gcgatcgccg
     5461 atcccaccct ccagagccgc tgcgactggg gccctcgtcc cggccttctc atcgcccggt
     5521 ggcgctcgac acgaatacgg cgtagtcgtg agcgcccaga tacacctgcg gacgagtcag
     5581 gtccaaggcc ggtcgcgacg ggtgtagggc gtctcgcagg atcttgcgag tcgcgttccc
     5641 ggacggcttg actggagcga agcgtaggac cacgggacgg gcgccaaaat tcagcgcgac
     5701 caccgcgagc tgcgcgtcgc tcacgcgcgc aaaagcgagc ccctccccct tgggatccac
     5761 ggcgagcagc tccagctccc cgcgctgcag ctcacgttgc gcgtgacgca gggcgatcag
     5821 tttgcgatga aagtcgtaga gttcggggtg gggcggggca acgatcggcg tgagtttgga
     5881 gtatggctcg aactcggctc cttcctcatc gaaagaatag agtgcaggaa ctcccggcaa
     5941 cgtgaataga gcgacggtgg ctacccgagt cagcggcaaa ccatagcgcg taatgaagcg
     6001 tggccccgtg tcgttgttgt tcaagaagcg caccacctga tgcacatcgt gtttgccagc
     6061 ctgtaaggcg gactggagca cgttcgcgaa gcgcgccgcg attccgctct gactcgtgaa
     6121 gacctcctcg aacgcccagt gtccgagttc cgggccccag tcgtaggcga agtcgaagcc
     6181 ctgctccacg tagtaggcgt cgcggcccgg tgcttctgcc agcatccagg cacaaggctg
     6241 gatcgtgctc agttgccgca gccaaggagc aaagaatgcg ggctcgcgct cgcggattcc
     6301 ccacgccgca tccacgcggt agccatcgac gtcgaagtct tcgagccagt aggatgcggc
     6361 ctcactcatg aagcgccgaa cttcggcgtt gccatagtcg aggttcggca aatgctgcca
     6421 atcgaagtag tgggtcgata gcccatccgg ggttctttgg tagaacccga agtaatgtga
     6481 cctcgcgccg agctccatgc cctggtgaaa gtagcgatgg gtgctggagg tgtggttggg
     6541 aaccagatcg agaatcacgc gcattccgag gcggtgggca gacccaatca gagacttcaa
     6601 atctccaggg gtgccgtagt cggagcgcac tcgaaaatag tcctcgacgg cgtaaccgaa
     6661 gtctccggct ttgttttcga aggtgggcgc gagccacaac acgtccacgc ctagctccgc
     6721 gagtttcgac agccgctgtt ccactgctct taatggttgg gcgccgaata ggggaggcag
     6781 cacaccatac aaaaccgagc cggcgaacag ctctcgacgc ttctcgcgat cggcgcacga
     6841 ccagggctga ccgtcacgta cctgaatgcc cacgctcgcg ctgccgacct cgccgtcagc
     6901 gtcggtcacg gtcaatcggt agatcgaact gccgtcagcc ggtgcgctgc cggtccacgc
     6961 ggggccgctg cccagcgtgt tgaacggccc tcccagccga gtgcgttgag accagcgata
     7021 ttcccggagc ggggagttgc tctctgaatt cacggcgctt tgtcgggcgc tcagctcgat
     7081 ctcgcccccg cgcaccgtca gcgctatggc cgcgtggggc tgcggcttca gccgtacatc
     7141 gtagctctcg ggattggacg cgatcactcc accggccgcg tcgcgacagc tagccacgac
     7201 ttgattgagc cctggttgaa gcctcgcgtg tgcctcgtaa aagaccgaac cacgcgtcgc
     7261 gggaagagcg cgagatccga cccggaagga gcaatcgagt ggggcctgtg gtccgtcgtg
     7321 tttcgttcgc agctcgagcg caaacgtgaa cagctccgcg ctggccggcc atagctgcaa
     7381 ttccgacgtc ctggcgctct gccgttcgcc ggccccggtg ggctgcatgc gaactggcgc
     7441 gttgcacgcg cagaacatgg cgcaactcga gctccaacgc cacagcgaag agcgcagcat
     7501 gactgtgtta tgccggctcg cgtggccagg gtccacagga attccgcact gcactcgagt
     7561 gttggattag ccgcctgcgg ccttgggtcc caagcgagcg gcatatcacg cacagtggac
     7621 tgcggggcat gctgggcaaa ggcaatgctg ggtgcaagca atgctcggtg caggcaatgc
     7681 aggagcaacg aggcgcgagt gccgtcatct ctgtcgtaaa ctgtcttcag ggcgggtggg
     7741 gttggcgatt gagattcccc gggagtaacg attccaggcg cccctctcag tatcgaaggc
     7801 gagccgtcga gtgatcggga gatggcaccg gacctgcagg gcttggccgc gcagtccact
     7861 tgtttatttc gcgcacccgc agaaaatcta gcgcacatgg cgcgttttgg agagcaacca
     7921 tgaacgtcag tgactacgta ctcgaacgac tcgtcgcctg gggtgtacgc cgggtatttg
     7981 gctatcctgg ggatgggatc aacggtctca tgggggcgtt caatcgacgc aagcaggcgg
     8041 acctgcgctt catccaagcg cggcatgaag agaccgcggc cttcatggcc tgtggtcacg
     8101 cgaaattcac cggcgaggtc ggcgtgtgct tggcgacgtc gggcccgggt gcgatccacc
     8161 tcttgaatgg gttatacgac gccaaaatgg accacgtacc cgtggtcgct ttggttgggc
     8221 agacggcgcg cagtgcgctg ggaggagatt accagcagga ggtggatctc aagagcctat
     8281 tcaaggatgt ggctcacgaa tacgtgcata cgtgcatgac tccagagtcg ataaggcacc
     8341 tcgtggatcg cgcttttcgc actgcctccg cggaacgcac cgtcacctgt gtgattcttc
     8401 caaaggacgt gcaggagttg gagttcgaac ctccgcccca cgctcacgac acgatccact
     8461 ccgcgccggg cttctcccgc ccacgagtga tacctcgaac caccgatatc gagcgcgcgg
     8521 cggagattct caatgcggcc gagcgagtcg cgatgctggt cggcaatgga gctcgcgacg
     8581 ctaccgaaca agtggtcgcg gtagcggagc gcctctcggc cggcgtcgcc aaggccttgc
     8641 tcggccgcac ggtggtgccg gacgacttgc cgttcgtaac tggttcgatt ggattgattg
     8701 gcaccgagcc gagctggaaa ttgatgcagg agtgcgacgc gctgctgatg gttggatcga
     8761 gcttcccgta ttcggagttc ctccccaaag agggaaaagc gcgcggcatt cagattgata
     8821 tcgacggccg aatgctcggc atccgctatc ccatggagct caatttggtg ggcgatgcgc
     8881 gcgagacact agagttgttg ttgccgcacc tccgccaaaa ggagcacggc gagttccgga
     8941 gaaatattct ccaatggaat gccgattggg agaagctctc ggagcagcgc gcgagcactc
     9001 aagccaatcc catcaatccg cagctcgtct ttcgcgaggg ctccaagtat ctccccgatc
     9061 gctgcatcat cagcgcagac tctggttccg cagccgcctg gtatgctcgt gacttgaagc
     9121 tgcggagtgg gatgctggcc agtctttccg gctcgctcgc gaccatgggc ccggcggtgc
     9181 cctacgcgat tgcggcaaaa ttcgcccatc ctgatcgtcc cgccatcgcc ttcgtggggg
     9241 atggcgcgat gcagatgaac ggtaacgcag agctactcac gatcgcacgc cattggcgcg
     9301 aatgggcaga tccgaggctc gtcgtgttgg tactccacaa caacgacttg aatttcgtga
     9361 cttgggaaat gcgcgccatg gccggagacg cgcgctacga agcgtcgcaa gatctgccag
     9421 agttcccata tgcggcctac gcagagttga ttggactgcg cggaatacag gtagaacagc
     9481 ccaaccaagt cacggatgca tggcggcaag ccttgcaggc ggacaggccg gtagtcatgg
     9541 atgtccgggt cgatccggat gtgccgccaa tgcctcccca cgtgaccctc aaacaggcga
     9601 aggcctttgc gatgagtcta cagcaagagg gaagccccgg cggagtgatc aagcaggtga
     9661 ttcagcaggt atttccgaag aagccgacgt gaccacgtcc ctcgcggagc gggagcacga
     9721 gaaaaggcgc agccgcgcct catgagatca atttgattac cgcctagcga cccaacgctc
     9781 gagtcggggc gctaggcggt gtgatgccga tgtgtcggtc gttcgttgcg gctttcagac
     9841 cgttgcttgg ctctaggcct tgccggtgag cgcatctcgg gcgcgatcga gcatggaagc
     9901 gaaggggccc gcgagcatgt tagcggccgc cgactcgacg ccggggtgcg gccgtgtcgg
     9961 tgcgatgctc tcggcgaaag tcaccgcctt gcgcatcttc tgcaagcgtt cgggctccat
    10021 ctgattgccg agccgatcga actcttccat ttcctcggcc tcggcgtggg cgatcacgct
    10081 ggcttgcaag gtcgtgaatt gaacctcgaa gtccatcgag tcgacatcca tgtcctctag
    10141 ctgtgagagc acctgcttgg ccgcgttctc ctcctcgagg cgcgcctgaa ccacgctttc
    10201 gccgtcggat agggccttac gtgcggcagg gtggacaatc tcctcttccg ccgtctcgtg
    10261 tacggcgagc agacgccgca gagcatagaa ggccgtgcgc cgaggttcgc cgctggtcgc
    10321 gagcacttcc ttgaaaccag ctttgatttg ctggtgctgc tccttcaaga acgtcaccac
    10381 atcgatgcca ttactgatca ttcttgccat ggggataccc ttctgacctt tcctcaatcg
    10441 atggctggcc aggctggcgg cgtcgtcagg tcgcttcgag cacgagctcc tgaagcaccg
    10501 ttacccagtt caccagcacg agcgcgagac ggtcctcgat tcgctgcaac cctcgtgccc
    10561 taccgatctc acaccagccg cgttgcccat ggcgcgcgac ggagcgctcg ctaggcatgc
    10621 ggcggcatcc tgattgcata caatgctcgc gccgcgcgaa gctttatcgt gcgcggtgtt
    10681 gcgtgcggca attcgatgcc gacgcgggag gtccaaagtg gcagcaagca agaagaacgt
    10741 atccggtagc gcacgagcaa ccatcgatca cgacgagatc cgcgagtggg tggaagctca
    10801 cggcggtcat ccggctcatg taaagagcac cgcccgaggc aagtcctcag gcattctgcg
    10861 catcgatttt ccaggattct ccggagaagg agcgctcgag cccgtcgaat gggaggagtt
    10921 cttcgagcac ttcgagaatt cacagctggc ttttctctac caagacaaga ctgcctcggg
    10981 acggccgagc aacttcaaca agctcgtgaa gcgggacagc gtagagctca cgccgtcacg
    11041 gacgcgcaag tcgagttcac ggaagagcac gggcgcccgt gcgcaagctt ccgcccgtaa
    11101 ggcgactccc cgtaaggcca ccgcacgtaa aggagcagcc cgcaaagccg cgtcccgttc
    11161 cagctcgact cgttccagct caagcgtgaa gaagactagt tctcgccgcg gtgcaaccgg
    11221 cgggtcgagc acgggtcgga gcgctagtag cgggtcgagc tccggtcgcg cgagttctcg
    11281 atcgaaaacg ggccgaaagg ctggcgggca gaatcagaca ggccgacgcg gagccgcggg
    11341 cacccgttcg gggcgctcgc agtccggcaa gacggcgcgt gcaccgtcgc aacgagacgc
    11401 ccccaagcgc gtcgccaaga aggccggtgg ttcgcgcgcg cgcaaaacat ccaagaagaa
    11461 gtaagtttga tgcctgacgc aggagcaaca ggcactcagc cctttgggga gaaacgccat
    11521 gacactacct caccctaaat ccagctctga ccgcgcctcg tcagggccgc ggacaacagg
    11581 gtcgctaaca acagggccgc ggaccgccga gtctcgctct actcgctctt cgaaaggcga
    11641 gacgtcttcg gctcccggtg atgaagacga gttagacgag agcttggagg acgatgagtt
    11701 ggacgaggac ctcgacgacg atgatttgga cgaagagctg gacgaagact cagaggatga
    11761 gttggacgac gactcagacg atgaggactt cgatgacgag gattcggacg acgaagactt
    11821 cgaggacccc gccaacctga gcgcggacgc cgcggacgac gaggatgctg ccctaaggca
    11881 atccatcagc gacgggtacg acgccgatgc tggcccactg gagaccggct atgcctccga
    11941 ggcggtgccg ctctccgaga tcctaaacaa tgccgcgctc gatgatgaat cattgagtct
    12001 cgagcccgag gacctggggc gccatgcgct gcaaagcgcg gcgcagcagg gcgacccaat
    12061 tgcgagcccc gctcgcatag cgctcgacga agcagagccg aatgcgagcc ccaatcccca
    12121 agctttgacg gagccaagca acgtcgacct cacgcaaagc gcgatccgcg agggatcgct
    12181 gttcgatcag gcccgcgagg gagaagagac gaaactccgc catccgctgg tcaacaccaa
    12241 cgaagtcgat gcgggactga agctcaacga acgagctcag cgagcgggca cgaagaccgc
    12301 gttgcccggg cgagtcgcgt cggagcagac tgcaactcgg ggaaacgtaa agaaacctgg
    12361 cgcaaccacg cccaagtccg ctaaaaccag ttccgcgaaa gatggatcgg cgcggtctcg
    12421 gcgaagctga gtatgccctg aagacgttca atccgagtcg gtatcgttcg gaaccgctag
    12481 attcagcgcg cacgtcagtc gaagtggtcc gtcgcgggca acggttcggt tcattccgat
    12541 tcgacccgcg gcggccctcg tactacctgg tatcgctcgc gtcttgtgtc ggaatgctcg
    12601 catggccgac agccgcgctg gcggaagacg gcggcaacga agtgtgtccc gccaactacg
    12661 cctccgcaaa tgatgttcgg gagcgttacg atgcgcgagg ccgcttggtg caccagctcc
    12721 gcctcgaggc cgggcaacaa gtcgaggaga tcgccattca atatgggggc gctcacgcga
    12781 ccgagcgcac ggagctcacg ccggggcgcc ggcgggttgc caaaacgcag tggaatggtg
    12841 attcggtcac actcgcagag tgttacgtcg acggcgtcct cacggcgagt gccacctatg
    12901 gatatcagga tacccgagtt gccctcgtca cgcggcgctt ctacgagaag ccagccacgg
    12961 agcgcgtcga gaccactcgc ttcttctacg actacgacgg acagttgatt ggaaccgagc
    13021 tgagagatgg ggagcaacga ctgctgagca cggtgcgcgc ggaacgtgcg ccgctgcgcg
    13081 ttccagtgca gctcagcttc aacgcgggtg gttcctatca gacggacacg aacctatacg
    13141 acgtcacggc ggggttcggt attcatcggc gcccgcaagc gcatcgctat ggagccgatc
    13201 cgctcgaggt cgagctcgac ggcaacttta gattccatcg cgccgctggc attaccagca
    13261 ccgatcaaac gacggcacgg ttcagtgcgg attatcacga aatcttgccg cacgttaccc
    13321 tattcacgtt caccgccagt gaccgcaact tgcccgccaa tcttcggctc aatctcgagc
    13381 aggcgttgct gggagtgaag tacgatttct tgccgcgcac cgactatcaa ctcgacatta
    13441 gctttgcgcc catttggaat ttccggtcaa tcatcgctcc tcctccggcc ggtgaagagg
    13501 gcgcgcccag cgtcaacgag actacatcga agcttcgagg atctttgcgt gccagagcgg
    13561 gtgtacaccg caccacgtgg gcgctgcgcg ataccttcga gttcctgccc acactctatg
    13621 gcgacgacgt gccacaggag aacggctttt ggaatcgagc catggttcga aacaccgtca
    13681 ccctagagct caatttcacg ccctatctga gcttacgtga agagttcaag tatacgcgcg
    13741 atccagccat gcgcgcccag gcgagctgcc ccgatgactc caatccgctg tgtcggggct
    13801 acagcatcgt gagcaccagc tcgatcgtat tgcgcctcga cctttgaacc tatcactcga
    13861 gccgcaaaat agcggctagc gcccggcgcc caacacggcc atgacccgga aatagacgtc
    13921 gatgttctcg tacgtaccta cgaaacgtcg atgcgcctct tctttcggag cgtaggcgga
    13981 tacgggcaca tcggaagcgc cgtgcggagc attgcccgac tttacccctt gcccgcgcac
    14041 gaacatgccg gccgcgcgat cccaagcgcc gagcccgtcc ggcccaggca gcggccgagt
    14101 cagccaccgt tcgaaacgat cggcgctcgc gccgtagccg attagcatct tgccgtcgac
    14161 gttgaagttc tcgggatagc cgtcgggtag aatctcgtag gttggaaatc ctccgttcgt
    14221 gaagttcgtg ccgaccacct tctgccgttc gggttgcacg tctggggaca gaacgccggc
    14281 ctccgacggg agtgcttgca ggtagctcaa actgccaatg ttggtattgg tcacgggatt
    14341 gcgcaatcct cccatagcca cgaaacctcc gcactcgtgg tctgccaata cgagcaccag
    14401 agtattgcca tcggtctccg cgaagcggcg cgcaacaccc accgctcgat cgaactcaat
    14461 cgcatccgcg atggcccggt ccgagtcttg agtatgctct tgcttgtcga tcgaggctcc
    14521 ttcgaccagg agcgcgaacc ccttgtcatg tttgctcaac acgcggagcg cggcgtcggt
    14581 catctcgtct aacatgggct gattggggaa gccgtcgttg tcagcggcaa acgggctctt
    14641 cggtggcgtg aattggttgg cgaactcggt cgcgccgtcc acgggcactc cgcgtctcct
    14701 cgcgatcttg tctaccgcga cgttcatatt tgccaagtgg aagagcccta acagcatgct
    14761 gggtacctcc tgcgccagcg ccgcgtccag ttgcgccttg tccgcgacgt acgtgaagcc
    14821 cgccgtgccg aaatccgcga tcagatcgcg ctgcgggtcc agtgctccgg cggcggcggg
    14881 tggcaagctc caggccgcca ccagatcggc aggcaacgcg gggtaatccg tagccgcagt
    14941 tcgagccgag ccggctgcgg actgaggcaa gaaccacttg cgcccgccgc ccagcagtac
    15001 tcgaagcccc gtgccggata cgcccgatcg gttgggatct gattcgtcca aaaactgatc
    15061 gcagatgccg gttcccgcgt tgcggtcgcc cgtatgcacg gccatggccc ccggagtcgc
    15121 gtcctctacg tcggcggtcg tgacgatgcc tgagatcttg ccctgcgtgc gatgcagata
    15181 ctcggccatg ttctcgacac gaggctgaaa gaacgggcta acgacctgcg cggggaacac
    15241 accttcttga ttgttgttgt tatgggtccc ggtgctgtaa gcggcgaacc cgggtgccga
    15301 atcagtcagg tagttgttga gtgaatgggt cgtcaccaag ccggtgctcg ggaagcgatc
    15361 catctcgagc caacctcggg tcgtgccgcg tgccacgccg aagcgtgcta cgcgcgcggc
    15421 ggtccgcacc ccaactccca ttccgtctcc gagtagcagg atcacgttct tgaggcggtg
    15481 cccgggtggg gcggcgtcga ggtcttcaat caagaagtgg ctgcgtatgc gtttggtgcc
    15541 cagcgagctg ctgaaggtgg cgtccagctc gtgcacaccc gccttggtgt tcgagtagcc
    15601 acgcagattg aagcctcccc agccggccgt ggtgatgccg tccgtgccgt tggaatcctc
    15661 cgcacccgag ctgaatgcac ggagttcccc gtcaatcttc aggctcgccg cgtagcgggg
    15721 gttgggagcc gttccggttc cttccacgcg aatatcgaag cgtgctcccc ggaaaaagcg
    15781 cgcaccatgc ggcggccaaa cattgacggt gggcccccca ttcagtggct cgacgagcgg
    15841 atcgggaggc atgctcgggg gcgcacacgc tttggcggtc acgagtggtt cgacgatcgc
    15901 gctcggtgct tgctccgtct tgacctcgcc tccgcacacg ctcagcccaa ggcagcaccc
    15961 tgcggtccaa accggtactt tctgcataga gaactcctgc tgtgtgatct tcgaattcaa
    16021 gccgatcccg cgagaactta cacagccgag ctgtggcccg ttctgaaagt ccaatactag
    16081 agctaactag ccgctacccg cgcggtgccg gtactttgag cgtggtgcgc gttctcgcaa
    16141 accggatccc tcgggcgtcg tccacgatta accgaaccga gctaatctcg ccggctttct
    16201 cggcggtgcc caactcgaat gttccactga cttcgagggc tccgggtacg aagtcgatgg
    16261 ggcgcgtacc tgccgcggag agcagcacga gcacggcagt tggcggaact ccaccgcgcc
    16321 ccgcgccaga ctcatcactg attgcggggt agggagagag gtagaatccg ccgtgcaccg
    16381 ggttctccag caagaccatg aagcccacga gtgtcccgcg ttttccagcg agatcacgcg
    16441 cccgatccgt gagttccggt gcgcgatctc caaagcggta gagttcccga aagtcgagtg
    16501 ttgccatcga gcgtgctcga gggggggctc gagggacgag tcgaaagctg ctctgtgttt
    16561 gcccgcgttg cgcagttccg cgttcgctat gccgagtggt tctgtgacgc ggcgttggag
    16621 cgagccctcg gagaatcgtg tgcatgactc ccggtcgaga ccaggatagc gaccagaaac
    16681 cacacccacc gagctaaccc agagcaatac ttcgcccaat catactccgc ccagccatgg
    16741 ttggcccaac catacattgc gctcgcctcc ctccatgctg aatcccaggc atgacccgct
    16801 tcagatgcgc tccaggcctg tttcgagctt ggcacagact ccagaacggc tcagtcagaa
    16861 actctaacga ttcgtgacac gatgtctacc gtagcttccc gcgctccgcg attgagagcg
    16921 gatttactac gccatgaaac aaacatgcag gctcgagatc ctgctttagc gactattcca
    16981 agtacgctcg atcgacagtg gtccgcgcat cccggccggg ctcgtacgtt actaaagcgt
    17041 attctgccgg ggagagtcct actctgtgag cgcaccgacg agctgtctaa acgtcgattg
    17101 ttggtctcgc ccgacctcgt ggacgtagat tctctggaag ccaaggttct gatccgcgcg
    17161 aaactcaccc accaactcct cgacgtcgtt cgtcactcgc acggagctct ccacgtcctc
    17221 agggcgcacg aagcgagcgg cctcgtcgag ttgctcgggt accgcgacgt cccaaagcag
    17281 tgggccggcg agcgcgccag ttcgccactg ttcgtgcgcg gcttcgagcg cggcacgccg
    17341 ctgcttcgcc aacgcaacgt gaacttgcag gtacatgggt ttgccggcgc cgccgttttc
    17401 gcggaaggcc tcgaccacac gcgcgagctg ctcgcgaggt tggtttaccg taatcagacc
    17461 gtctgcccag cgtgccgccc aagccgccgt ctcggcgctc accgcggcac cgaatagcgg
    17521 cggaggcacc tctgggcggg tgtacaattt ggcgcgctcc acggttacga gccccgcgtg
    17581 cgtgaccgtt tctcccgcga atagcgcacg catgacctcg acgcattccc gaagccgcgc
    17641 ctggcgctgt gccttcactg gccaatgctg ccccgtgatg tgctcgttca aattctcccc
    17701 gctgccgagc gccagccaaa atctccccgg aaacatctgc gttagagttg ctgctccttg
    17761 ggcgacaatc gcaggatggt agcgctgacc gggcgcggtc acggttccga aggtcatgcg
    17821 cgtggcctgc agcgccgcgc ccaaccaaga ccaagcaaac ccgctttgcc cctgcgccgt
    17881 gctgaacggt gaaaagtggt cggagcacat gccatgcagg attcctagtc cgtccgcatg
    17941 gcgcaccgcc tcgagcagag cgctgggcgg gaattgctcg tgcgaagcgt gatagccgaa
    18001 ctcgatggtc atgaccgttc tcccgttcga ctaaacatat gctgtgttcg cccttcaggg
    18061 gactgcgtgt ttttgcgcag tgcgggcctg ccgtggcggt tgccgtgcaa atcgccacgg
    18121 cgcattaggc ggcgggcaac acgcagcatc agggatggat cgcgctcgca aaaatgcgcg
    18181 aggatgagcg cgtgattaag gatctctggt acaaaaacgc ggtcatctac tgtttgcacg
    18241 tcggcacgtt catggactcg aacgccgatg gcatcgggga cttcgaaggt ctcgtgcgac
    18301 ggctcgatta cctcgccggt ttgggcgtaa cgtgcttgtg gctccatcct tttcagaagt
    18361 cgcctcgccg tgatgacggc tacgacatca gcgacttcta cacggtggac ccagtgtttg
    18421 gcacgctcgg agacttcgta cagttcacgc acgaggccaa gcagcgcgga atccgagtga
    18481 tcatcgatct ggtcgtgaat cacacctcgg atcagcaccc ctggttcaag gcggcgcgca
    18541 aaaacaaaca atccaagtac cgagactatt acatctggtc ggccaagcgc cccaaggacg
    18601 cggacaccgg catggtcttc cccggggtgc aaaagacgac ttggacgcgc gactcggaat
    18661 cgggcgagta ttacttccac cgcttctacg agttccaacc ggacctcaac acctcgaacc
    18721 cggccgtcca ggaggagatc cgcaaaatca tggggttctg gctccagctc ggcgtcgcgg
    18781 gttttagaat ggacgccatc ccgttcgtga ttcaaacgaa gggggcgggc atcaaga
//
LOCUS       DASTKU010001090         6234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1109247, whole genome shotgun sequence.
ACCESSION   DASTKU010001090 DASTKU010000000
VERSION     DASTKU010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109247"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VFQ61_39000"
     CDS             <1..924
                     /locus_tag="VFQ61_39000"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HET9960543.1"
                     /translation="REEMGEENYRRARQILADLDAVGNTTKAETKGIAIGSAVIAAVS
                     LFASFIAVMAVGSEDKIAQMSSSQYLEEAGKLTVAHPMVFIGFLLGGVVPFLFSSMLI
                     RAVGRAAFLIVKECRVQFRDKEIWAGTKKPDYGRVVNICTVSAQKELVGPGFLAICTP
                     VVVGFLLGTQALGGFLAGMILCGQVLAVFMSNAGGAWDNAKKFIEDGNYGGKGSESHK
                     AAVTGDTVGDPLKDTAGPAVNPLIKVMNMVSLLTLGLVMNYNVITPTGGAQARAVGLG
                     VALIALGAIVWAVWQSKRETAELRDMEEQFR"
     gene            complement(1052..1258)
                     /locus_tag="VFQ61_39005"
     CDS             complement(1052..1258)
                     /locus_tag="VFQ61_39005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960544.1"
                     /translation="MVTRAEYLSQFQSPADAAEFESQVLDLEGQSLPAFAKVARDKSA
                     EPSSEYWAWLLEHLERLQLGEHEE"
     gene            complement(1290..3113)
                     /locus_tag="VFQ61_39010"
     CDS             complement(1290..3113)
                     /locus_tag="VFQ61_39010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960545.1"
                     /translation="MSTSVTHCSNLPHSFHIPVMGIGFTLDTPVAVAPLGLSSVISLV
                     DDVLLEEVRAHFLRERGEPYVAITDEDPDPRAHRVTAYLNLIDRMARERFEWLRGSTF
                     EDPKGIRRYFELLPEGPDRQAYLQFLATPEGPERRQLEAELRARVVMGSIDVNIMTKL
                     DRQLDNRGLERPAGQSDAVAALRGFAESTLAGNVVLSAGMNPRMFAIFAQYADFFPQG
                     GMPPKKGVIIKVSDFRSAAVQGRFLARRGVWPKEFRIESGLNCGGHAFPTDGVLLGPI
                     LEEFRTGRDQLAEELFSVYKKSKENPAAATSPMPALRVTVQGGIGTASEDRLLRTRFG
                     MDGTGWASPFLLVREVSRTSEDCLARLTNPQTAKVVLTWGSPLGVPFWSLENSGSDRA
                     RRERIRAGRPGSACPKGFLALHAGLAKVPLCAASRAYQKIKLAEVDPAEPAREAVMAR
                     ITEPQCICHDLGGGIRQTFGIDPAATPSICPGPNIRFFTREYSLDEMVGHIYGRLDLL
                     EGVRRPHQFIQELALYVDFLRDRLAEIGTPLNDKKVQDYESYRQHLSEGIAFYDRLVP
                     DLPAAERAAFREGLREQEARLDSLRRVCPVSSEAAPMQASG"
     gene            complement(3292..4476)
                     /locus_tag="VFQ61_39015"
     CDS             complement(3292..4476)
                     /locus_tag="VFQ61_39015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960546.1"
                     /translation="MKLVIPSSTFKLATSIAAVLMFACASDASKPEATGGSSSLPGQG
                     GTTTSSGGIPGAGGAAPGASGAATSGGGVSTGGTRAISSAGPGTSGGTSPNEGGSVSG
                     GTSSGGGTSGGTAPGGSLSGGNASAGSSNGGASGGSANAQGGASSSAGEVGSSGAAGA
                     SGQGGTINDPKLAGDFDGALIAYPCGAAHSGFDCDNIGCKNGQVTHTQTFKMGGVSGT
                     VYDVTFRVRGVVEAYNYVGGTRDAGNDSINKTRDLFLRGGAPQPGGANGYDYDVYELN
                     VSPPVSGAPATYFLNSVSNAENPHTSSATLHLSFDIDYTKTIQVGGGATITVKQYDSN
                     CKSVMNCGPSASGSCPAPRKVSLEGASPAPPTTFTQPYQMPTGAYGQWLFFDVTSVTV
                     AR"
     gene            complement(4891..5883)
                     /gene="gap"
                     /locus_tag="VFQ61_39020"
     CDS             complement(4891..5883)
                     /gene="gap"
                     /locus_tag="VFQ61_39020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET9960547.1"
                     /translation="MIKIGINGFGRIGRTAFRAAAARSDVEIVAVNDVMQPSHLAYLL
                     KHDTVQGRFAAPVEAKDGLLVVAGRPIRVTATKDPAAIDWARAGVDVVIESTGIFLSR
                     DGAAGHLSAGARRVIISAPTKDDTAVFVHGVNTNEYAGQPIVSAASCTTNCLAPIAKV
                     LHESFGIQRGLMTTVHATTATQKVVDGVSGKDWRFGRGILENIIPASTGAAEAVAKVL
                     PELKGKLTGMAFRVPVSNGSVVDLTCELQRPTKYAEICSAMKEASRGAMKGVLAYTEE
                     ELVSSDVRGEACTAVFDAKAGLQLDPTFVKIVAWYDNEWGYASKLLDLAIKVAA"
     gene            complement(5988..>6234)
                     /locus_tag="VFQ61_39025"
     CDS             complement(5988..>6234)
                     /locus_tag="VFQ61_39025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960548.1"
                     /translation="VMALLGTPVRGGVIGGVDLVDTDYGALAIDSKTGEGSDGGDIDP
                     LTSLESAGKTLCAALELPADTIAKRITTGKVVTAALV"
BASE COUNT         1145 a         1955 c         1956 g         1178 t
ORIGIN      
        1 cgcgaggaga tgggcgagga gaactacagg cgcgcccgcc agatcctcgc cgacctcgac
       61 gcggtgggca acacgaccaa ggccgagacc aagggcatcg ccatcggctc ggccgtgatc
      121 gccgccgtct ctctcttcgc ctcgttcatc gcggtcatgg cggtgggcag cgaagacaag
      181 atcgcgcaaa tgagcagctc gcagtacctg gaggaggcgg gcaagctcac ggtggcgcac
      241 ccgatggtct tcatcgggtt cctgttgggc ggcgtcgtgc cgttcctgtt cagctcgatg
      301 ctgattcgag ccgtaggccg cgcggcattc ctaatcgtga aggagtgtcg cgtgcagttc
      361 cgagacaagg agatttgggc aggcaccaag aagccggact acggccgtgt ggtgaacatc
      421 tgcacggtca gcgcccagaa ggagctcgtg gggcctggct tcctcgccat ctgcacgccg
      481 gtggtggtgg gctttctgct cggcactcaa gcactcggtg gattcctcgc cgggatgatt
      541 ctgtgtgggc aggtgctcgc cgtattcatg tccaacgccg gtggtgcttg ggacaacgcc
      601 aagaagttca tcgaggacgg caactatggt ggcaaaggct ccgagtccca taaggccgcg
      661 gtcacgggcg acacggtagg tgatccgctg aaagacacgg cgggcccggc ggtgaacccg
      721 ctcatcaagg tgatgaacat ggttagcctg ctcacgctcg gcttggtcat gaactacaac
      781 gtgatcacgc cgaccggcgg cgcccaagcg cgcgcggtgg gcctgggcgt cgcgttaatc
      841 gccttgggcg ccatcgtctg ggccgtctgg cagagcaagc gcgagaccgc cgagctgcgc
      901 gacatggaag agcagttccg ttagacggaa accgcgactc gagatcgggt ggggctcaga
      961 agcgagggga ccggtaacgg tcccctcgtt tcatttcagc tcgtcgcaga ggggtccgta
     1021 gcgcgagcga cggtttgcag cactcgatag gctattcctc gtgctctccg agctgcagcc
     1081 gctccaagtg ttcgagcagc caagcccagt actcggagct gggctcagcg ctcttgtcgc
     1141 gcgccacctt ggcgaacgca ggcagcgact gaccctcgag atcgaggacc tggctctcga
     1201 actccgcggc gtccgcgggc gattgaaatt gggataggta ttctgctcgg gtgaccatct
     1261 ggggactgcc ctagcacttc ttcctgactt caaccgctcg cctgcatcgg agccgcctcc
     1321 gaggagacgg ggcagacgcg gcgcaggctg tcgagccgcg cttcttgttc acgcaggcct
     1381 tcgcggaacg ccgcccgttc ggcagccggt aggtccggca ccagccggtc gtagaacgca
     1441 atgccctcgc tcaagtgttg ccggtacgac tcgtagtcct gcaccttctt gtcattgagg
     1501 ggcgtgccaa tctccgccag tcggtctcgc aggaagtcga catagagcgc gagttcctgg
     1561 atgaactgat gcggacgccg tacgccttcc aacagatcga ggcgaccata aatgtgcccg
     1621 accatctcgt ccaggctgta ttcgcgcgtg aagaagcgga tgttgggccc tgggcagatg
     1681 gagggagttg ccgcgggatc gataccgaag gtctggcgaa tgccgcctcc gagatcgtgg
     1741 cagatgcact gaggctccgt gatccgcgcc atcaccgcct cgcgcgccgg ctcggcggga
     1801 tcgacttcgg cgagtttgat cttttgatag gcgcggctcg ctgcacatag cggcactttc
     1861 gccaggcccg cgtggagggc caggaagccc ttggggcacg cgctgcccgg gcgccccgct
     1921 cgaatgcgtt cgcgccgggc gcggtcgctc ccgctattct cgaggctcca aaagggaact
     1981 cccagtggcg agccccacgt gagcacgacc ttggcggtct gaggattggt caagcgagct
     2041 aggcagtctt ctgacgtccg agagacctca cgaacgagca aaaacggtga ggcccagccg
     2101 gtcccgtcca tcccgaatcg ggttctgagc aggcgatctt cggacgcggt tccgatcccg
     2161 ccttgcactg tgacgcgcag cgccggcatg gggctcgtgg cggcggcggg gttttccttg
     2221 gatttcttgt aaacactaaa gagctcctcc gcgagttggt ctcgccccgt gcgaaactct
     2281 tcgaggatcg gaccgagcag cacaccatcg gtggggaatg cgtgaccacc gcagttcaga
     2341 cccgactcga tccggaactc cttgggccac acgccacggc gcgccaagaa gcggccttgt
     2401 accgccgcag aacgaaagtc cgatactttg atgatgaccc cctttttcgg cggcatcccg
     2461 ccctgcggaa agaagtcagc gtattgggca aagatcgcga acatgcgcgg attcatgccc
     2521 gcggagagaa cgacgttgcc ggcgagtgta ctctccgcga atccgcgcaa tgcggcgacc
     2581 gcatccgact gccccgccgg tctttcgagg cccctattgt ccagctggcg atcgagcttg
     2641 gtcatgatgt tcacgtcgat cgaccccatg accacacgcg cgcgaagctc cgcctcgagc
     2701 tggcggcgtt cgggaccttc cggggtggcg aggaactgca gataggcctg ccgatctggc
     2761 ccctcgggca aaagctcgaa gtagcgacgg atgcccttcg ggtcttcgaa ggtcgagccc
     2821 cggagccact cgaagcgctc acgcgccatg cgatcgatga gattgagata ggccgttacc
     2881 cgatgggctc tggggtccgg atcttcatcg gtgatcgcca cgtacggctc gcctcgctcg
     2941 cgcaagaaat gcgcccgcac ctcctcgagc agaacatcgt ccacgagcga aatcaccgac
     3001 gagagaccga gtggagcgac cgcgactggc gtatccagcg tgaagccgat gcccatcacc
     3061 ggaatgtgaa agctgtgtgg cagattcgag caatgcgtaa cggaagtcga cataacgccc
     3121 gcgactctag acttcgatcg cgagcgcgct catcagagtt tggtcatctc gagctttcgg
     3181 ggcgtccgac ttgatcgatg tcagtacggc cggaggtcag tcgatgtcga cgctagccgt
     3241 cgaacttagt gtgtaagcat gaatgggtag gagctccggc cagtctccgc gtcaccgagc
     3301 caccgtgaca ctcgtaacat cgaagaagag ccactggccg taggcgccgg tgggcatttg
     3361 atagggctgc gtgaaggtgg ttggtggcgc cgggctcgcg ccttcgaggc tcaccttgcg
     3421 tggggcgggg cacgagccgg aagctgatgg gccacaattc atcaccgact tgcagttcga
     3481 gtcgtattgc ttgaccgtaa tggtggcgcc gccgcccacc tggatggttt tcgtataatc
     3541 gatgtcgaac gagaggtgga gggtcgcact gctcgtgtgg gggttttcgg cgttagacac
     3601 ggagttgagg aagtacgtgg cgggggctcc gctgaccggc ggggagacat tgagttcgta
     3661 tacgtcgtag tcgtagccgt tggctccccc gggctgcggt gcgccgccac gcaaaaatag
     3721 gtctctggtt ttgttgattg aatcgttgcc cgcatcgcgc gttccgccaa cgtaattgta
     3781 agcctcgacc acaccgcgaa cacggaaggt gacgtcgtaa accgtcccgc taacaccacc
     3841 catcttgaag gtctgcgtgt gcgtgacctg cccatttttg cagccgatgt tgtcgcaatc
     3901 gaagcccgaa tgggctgcgc cgcagggata ggcgatcaat gcgccgtcga agtcaccggc
     3961 aagcttggga tcgttgatcg tgccaccttg accggatgcc ccagctgcac cggagctccc
     4021 cacctcaccc gcgctggagc ttgctccgcc ctgcgcgttc gcgctgcctc cactcgcgcc
     4081 accattcgag ctgcctgcgc tagcgttgcc gccgctaaga cttccgcccg gtgccgtgcc
     4141 tccgctcgtg ccgcccccgc tcgaagtccc cccactcacg ctaccgccct cgtttggact
     4201 ggtgcctccg ctcgtgccag gcccagccga ggatatggcc cgggtgcctc cggtgcttac
     4261 gccgccgccg ctggttgctg cgccgctcgc gcccggtgcg gcgccgcccg cgccggggat
     4321 cccaccggaa gaggtcgtgg tccccccctg ccctggtaag ctcgaactgc cgcccgtcgc
     4381 ctccggcttc gaggcgtcgc tggcgcaagc aaacatgagc acggccgcaa tggatgtcgc
     4441 aagcttgaat gtagaagaag gtataacgag cttcattcga gttcggaatt caaccagggt
     4501 cgagtgaaca gttcgggaaa gaggcaggct ccccctcgtc atctcgagaa cacgctactc
     4561 ccccgccgaa tggcgcagaa tgcacacgtg gctccattca cgctgtgccc gggttttagt
     4621 gattccgtca tcaaatcgcg aacgggcgga gcttgatttc gtcatcgaaa ttgccgcccg
     4681 gatcgaaagc atgtttaccc cattttcttg tgcacaaaga gcgtcgatct cgcaatcacg
     4741 gccgtgttgg caattgtcca accgcccgtc gacagcggga caattgccca atcaggccgc
     4801 tacacgagac tcagcaggtt gcgcccttat cggcacagcc gcgaggcacc tcgcgatcag
     4861 agcgccccaa agacgtttcg aaggcggctt tcaagctgca accttgatcg ccaaatcgag
     4921 caacttggaa gcatagcccc actcgttgtc gtaccaagcc acgatcttta cgaaggtggg
     4981 atccagctgc aaccccgctt tggcatcgaa tacggccgtg cacgcttcac cacgaacgtc
     5041 ggacgagacc agctcttcct cggtgtaggc gagcactccc ttcatggctc cgcgcgaggc
     5101 ttccttcatt gccgaacaga tttccgcata cttggtcggg cgctggagtt cacaggtgag
     5161 gtcgacgacc gagccgttgg aaacgggaac gcggaacgcc atcccggtga gcttgccctt
     5221 caattccggg agcaccttgg cgacggcctc cgccgcgccg gtcgacgcgg ggataatgtt
     5281 ttccaagatc ccccggccaa aacgccagtc tttgcccgaa acgccatcca cgaccttttg
     5341 cgtcgcggtg gtcgcgtgaa cggtcgtcat gaggccgcgc tgaatcccaa acgattcgtg
     5401 cagcactttt gcgatggggg ccaagcagtt ggtggtgcaa gaggcagccg agacgatcgg
     5461 ttgaccagca tactcgttgg tattcacgcc gtgaacgaac acggccgtat cgtccttcgt
     5521 tggagccgag ataattactc gccgcgctcc ggcgctcaag tgccccgccg cgccgtcgcg
     5581 cgacaggaat atcccagttg actcaatcac gacgtccacc cctgccctgg cccaatcgat
     5641 ggcagccggg tctttcgtgg cggtcacccg aatcgggcgg cccgcaacga cgagaaggcc
     5701 gtcctttgct tcaacgggag ccgcgaagcg cccctgcacc gtgtcgtgct tgagcaagta
     5761 ggccaaatgg gaaggctgca tgacgtcgtt cactgcgacg atctcgacgt cgctccgagc
     5821 cgcagcggcg cggaatgccg tccttccgat tctcccgaaa ccgttgatac cgatttttat
     5881 catgagcgca cctatcgcgg gcgatcacgt tcactcgaag gatcgcgtcc gcgagtggtt
     5941 tcttacgccg aggtgcctct gtcctaaagg acaatagaat tgcaaattca gacgagcgcg
     6001 gccgtgacga ctttcccggt cgtgatccgt ttggcaatcg tgtcggcagg gagttcgagg
     6061 gcggcgcaca aggttttccc ggcggactcg agggaggtca gcggatcgat gtcgcccccg
     6121 tcgctaccct caccggtctt ggagtcgatg gcgagggcgc cgtagtcggt gtcgacgaga
     6181 tctacgccgc cgatgacccc gccccggacg ggtgtgccca agagggccat cacg
//
LOCUS       DASTKU010001091         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_438103, whole genome shotgun sequence.
ACCESSION   DASTKU010001091 DASTKU010000000
VERSION     DASTKU010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438103"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFQ61_39030"
     CDS             <1..722
                     /locus_tag="VFQ61_39030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9960549.1"
                     /translation="DSVHFLGQAFSRISNYLERITGLLEQRPAPSLTEAEAAISDARK
                     QARLAFLMSEVPQALVACKEGIGQVSNIVQALKAFSHQDQDEKSPDDLNAALRSALVM
                     ARHEYKNLAVVHEELGELPLVPCLITQLNQVFLNLIVNAAHAIADAGRAELGTITVRS
                     RVVDGCAEISIADDGCGIPAAIQHKIFEPFFTTKEVGRGTGQGLALARQMVVDRHGGS
                     IEFESEVGKGTSFKIRLPILE"
     gene            837..1352
                     /locus_tag="VFQ61_39035"
     CDS             837..1352
                     /locus_tag="VFQ61_39035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET9960550.1"
                     /translation="MKATQLLAHQHRKVEALFKRLKHGRSDPHAVLEELADSLAAHMA
                     IEQEIFYPRVKEAASDLVNESFEEHALAELGLKRLLATSSDAESFEARVSVLEELIHH
                     HVEEEEQELFPKVEKALEEEELNQLGKTMKARFEEVLKGGFAAAVPKTMAKTSADAAK
                     KRHPKRVPSAA"
     gene            complement(1459..>2071)
                     /locus_tag="VFQ61_39040"
     CDS             complement(1459..>2071)
                     /locus_tag="VFQ61_39040"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET9960551.1"
                     /translation="LQVDLATAAGVDELYAGIGGRPVEALLANAGRGLGRAFLDQDLA
                     DIFRVVNTNVSGTLHLIHRVGNDMRRRGSGRILITGSIAGFMPGTYQAVYNGTKAFLD
                     SFAFALRAELKDCGVTVTCLMPGATETEFFERADLMDTKLGTQRKQPPEEVAKIGFEA
                     MLRGDGDVVAGWQNKLRAALSHIAPSAALAEAHRKLAEPGTAR"
BASE COUNT          404 a          656 c          645 g          366 t
ORIGIN      
        1 gcgatagcgt gcactttctg ggacaggcgt tttcgcgcat cagcaactat ctggagcgca
       61 tcacggggtt gctcgagcaa cgcccggcgc ccagcttgac cgaggccgaa gcagccatct
      121 ccgatgctcg caagcaagcg cgcctcgcgt tcttgatgag cgaagtcccg caagcgctgg
      181 ttgcgtgcaa agagggaatc gggcaggtct cgaacatcgt tcaggcactg aaggcgttca
      241 gccaccaaga tcaggatgag aagagccccg atgatttgaa cgcggcgctg cgcagcgcgc
      301 tggtgatggc gcggcacgaa tataagaacc tggccgtggt gcacgaagag ctgggggagc
      361 tgccgctcgt tccctgtctc attacacaac taaaccaggt cttcttgaac ttgatcgtga
      421 atgccgccca cgccatcgcc gacgccggcc gagcggagct gggcacgatt acggtccgct
      481 cccgcgtcgt ggatggctgc gccgagatca gcattgccga tgacgggtgc ggcattccgg
      541 ctgcgatcca gcacaagatc ttcgagccgt tcttcacgac caaagaggtc ggtcgcggca
      601 ccggacaggg tctcgcgcta gcacgccaaa tggtggtcga ccgccatggg ggcagtatcg
      661 agttcgagag tgaagtcggc aagggcacca gcttcaagat ccggcttccg attttggagt
      721 gagcgctacc cgtgactccc cgctgaacct ccgccggcac gcggcttgca gtcgttcgtg
      781 attgcggccg gtcccgcgaa cagcccgcga ggatccggcg ccgcggaggt acacacatga
      841 aagcaaccca gctgctcgcc catcaacacc gtaaggtgga ggccctattc aagcgattga
      901 agcacggtcg ctcggatccg catgccgtgc tcgaagagct cgccgacagc ctcgctgctc
      961 atatggcgat cgagcaggag attttttacc cgcgagtgaa ggaggctgcc tcagacctcg
     1021 tgaacgagag cttcgaggag cacgcgctcg cggagctcgg cctcaagcgc ttgctcgcca
     1081 cgtcttcgga cgcagagtcg ttcgaggctc gcgtcagcgt gctcgaggag ctgatccacc
     1141 atcacgtcga agaagaggag caggagttgt tcccgaaggt ggagaaagcg ctagaagagg
     1201 aggagctcaa ccagctgggc aagaccatga aggctcgctt cgaggaagtt ttgaagggcg
     1261 gatttgctgc tgcggtaccg aagacgatgg caaaaacgtc cgctgacgcc gccaagaagc
     1321 gtcatcccaa gcgcgtcccc agcgccgctt gaacgcctcg cgggatccag cgctcgtcat
     1381 ctagaaaaag ctgcggcccc gcctgagcgg ggccgcgttc cgttcgctac acgcgccgcc
     1441 gcgtgcaccg ggttctactc agcgcgccgt accgggctcc gccaactttc ggtgagcctc
     1501 ggccagcgca gccgacggtg cgatatgcga cagggccgcg cgtaacttgt tctgccagcc
     1561 tgcgacgacg tccccgtcac cgcgcaacat ggcctcgaac ccgatttttg cgacctcctc
     1621 cggtggctgc ttcctctggg tgcctagctt ggtatccatg agatcagccc gctcgaagaa
     1681 ctccgtctcc gtggcgccgg gcatcaagca cgtgaccgtg acgccgcagt ctttcagctc
     1741 ggcacgcagc gcaaatgcga acgagtccag aaaagccttg gtaccgttgt acaccgcttg
     1801 ataggttcca ggcataaatc cggcgatgga tccggtgatc aggatgcggc ctgatccacg
     1861 gcggcgcatg tcgttgccga ctcggtgaat gaggtgcaag gtcccgctga cgttcgtgtt
     1921 caccacccgg aaaatgtcag cgagatcctg gtcgaggaat gctcgtccca gcccgcgccc
     1981 ggcgttggcg agcagcgcct ctaccggccg ccccccgatg cctgcgtaca actcgtccac
     2041 ccccgccgcg gttgcgaggt ctacctgcaa c
//
LOCUS       DASTKU010001092         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1334161, whole genome shotgun sequence.
ACCESSION   DASTKU010001092 DASTKU010000000
VERSION     DASTKU010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334161"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VFQ61_39045"
     CDS             <1..1036
                     /locus_tag="VFQ61_39045"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET9960552.1"
                     /translation="YPLGLRFCFVDPTPGSPAGELGPQVARDYDSIEAQDALASCDVV
                     TYEFESVPVRAAEALTAKTRVLPPPSALNVAQDRLSEKQCFRSLSIPTPEFAAVSSLK
                     ELQRACERIGLPAILKTRRLGYDGKGQARLESPRDLEPAFERIGGSPLILEGFVHFER
                     ELSLIGVRGRDGSTAFYPLLENQHRDGILRLTYAPARGVSEELRVKAERYMGALLEKL
                     DYVGVLTLELFQVQGELWANEMAPRVHNSGHFSIEGAETSQFENHLRAILGLPLGSTC
                     VRQPSAMLNLIGAIPAREDVLRVPGAHLHLYGKNPRPGRKVGHITLVAPTAGELETRL
                     ADLKKLAGAG"
     gene            complement(1116..1694)
                     /locus_tag="VFQ61_39050"
     CDS             complement(1116..1694)
                     /locus_tag="VFQ61_39050"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HET9960553.1"
                     /translation="MNITRRSLVGAGLLLLSCRRAASLPKLGQVPSFALKNQDDKPYG
                     AHELWGQVWICAFMFTRCPTICPRITRRMRALQLRARQLGIPVRWVSISVDPDNDSPS
                     VLAEYARRFGADLSNWTFLTGDFSTIQRTAVDGFKMALDGRADAKAENYGIVHGSHLV
                     LVDSVGEIRGYYRTEDEPDGERLLQDARSIVP"
     gene            2229..>2591
                     /locus_tag="VFQ61_39055"
     CDS             2229..>2591
                     /locus_tag="VFQ61_39055"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET9960554.1"
                     /translation="MNSSKATDAPNAAGGSDAKFDEYAAEYDRLLEQSISASGEGKEY
                     FLEYKLACLKRLGIGADAAILDYGCGVGNLTGHLSGFYPKVAGYDPSAQSLALARKRH
                     ARAEFYDRVEAIPNRAFDA"
BASE COUNT          504 a          815 c          804 g          468 t
ORIGIN      
        1 atatccgttg gggcttcgct tctgcttcgt agatccgacg ccgggctctc ccgcgggcga
       61 gttgggtcct caagtggcgc gcgactacga ctcgatcgag gcccaagacg cactcgcgag
      121 ctgcgacgtg gtgacgtacg agttcgagag cgtcccggtg cgtgccgcgg aagcgctcac
      181 ggcaaagact cgcgtgctgc cacctccgag cgctctgaac gtggctcaag atcgattgag
      241 cgagaagcaa tgcttccgct cgctctcgat tcccacaccc gagttcgcgg ccgtatccag
      301 cttgaaggag ctccaacgag cctgtgaacg catcggcctg cccgcgatct tgaaaacgcg
      361 gcgcctcggc tacgatggca agggtcaagc acgcttggag agcccccgag atctcgagcc
      421 cgcgttcgag cgcattggtg gcagccctct gattctggag gggttcgtgc acttcgagcg
      481 tgagctgagc ctgatcgggg tgcgcggccg cgacggctcc accgcatttt atccgctgct
      541 cgagaaccaa caccgcgacg gcattttgcg ccttacgtac gcaccggctc ggggtgtgag
      601 cgaagagctg cgcgtgaaag ccgagcgtta catgggcgcc ttgctggaga agctcgacta
      661 cgtgggtgta ctcacgctcg agttgttcca ggtccagggt gagctttggg ccaatgagat
      721 ggcgccccgt gtgcacaact ccggtcattt ctcgatcgaa ggcgccgaaa ccagtcagtt
      781 cgaaaaccac ttgcgagcaa tcttgggctt gccactcggc tcgacgtgcg tgcgtcagcc
      841 cagcgccatg ctcaacttga ttggagcaat cccagcgcga gaagacgtgc tgcgtgtgcc
      901 cggcgcccat ctacacctct atggcaagaa cccgcgtcct ggcagaaaag tggggcacat
      961 cacgctggtc gcgccaaccg cgggtgagct ggaaacgcgc ctcgcagatt tgaagaagct
     1021 ggccggagcg ggttaggagc aactccgcgg cacctcgagc gaacgaaggc gggagcgacg
     1081 agaaagccgc gaagcggcga accgctgaaa agttttcacg gcacgatgct acgcgcgtcc
     1141 tgcagcaatc gctcgccgtc gggctcgtcc tcggtccggt agtaaccgcg aatttcgccg
     1201 accgagtcta ccagcactag gtgcgagccg tgcacgatcc cgtagttctc tgccttcgcg
     1261 tcagctcgcc cgtccaaagc cattttgaaa ccgtcgacgg cggtacgctg gatcgtggaa
     1321 aagtcgccgg ttaggaacgt ccagttggaa agatcggccc cgaatcggcg cgcatactcc
     1381 gccaacactg acggcgaatc gttgtccgga tccacgctga tgctcaccca gcgcaccgga
     1441 ataccgagtt gccgagcgcg tagctgcaaa gcccgcatgc ggcgggtaat tcgggggcaa
     1501 atcgtcggac agcgcgtaaa catgaacgcg caaatccaga cctgccccca cagctcatgg
     1561 gcgccataag gcttgtcatc ctggttcttc agagcgaatg aaggcacctg cccgagcttc
     1621 ggcagagaag ccgcacgacg gcagctcagg agcaacaagc cagcaccgac taaggaacgt
     1681 ctcgtgatgt tcatttcgcg ccacctgagc ggataaattt gccttctagt cgcaacgggt
     1741 ataggtcgcc gctcactttg ccggacgaac cggctcgaaa ggacggaaat catgcgccgc
     1801 ctacgcaagt tgctgtcacg actccgcctg cccgttcccg cgttcgcgtg aaagcgtggg
     1861 gttcgcgatc gaccctgggc tctgcggacg ttgtcgacac gcgagacgcc tggtctcggc
     1921 ccggggctct gcgttctggc tttgcggccg tgcgcagcgt gagccgacgc gatacccgaa
     1981 gtacccgcgc cttcccgttg tgagctgtac cggctttgac gcggccacgt ctgtttctgc
     2041 gccagatttc ggccgcatat tgccccaagc cgccgcccac gggactgtgg gccacggcac
     2101 agatagcagc tgcacaacgc cgcccgatga tgcggcgtcg cacgaagaca cgctgcggac
     2161 gcctaaaaag cgcgattgag tgccgcgccc gatgcgcgtt gacgccaagc gctatataac
     2221 gtgcttgcgt gaattcatcc aaggctaccg acgcccctaa cgcggccggc ggctccgacg
     2281 ccaaattcga cgagtacgcc gcggaatacg atcgtttgct cgagcagagc atttctgcga
     2341 gcggagaagg caaagagtac ttcctcgagt acaagctcgc gtgtctgaaa cggttgggca
     2401 tcggcgcgga cgcggccatc ctggactacg gctgcggagt cggcaatctg acggggcatc
     2461 tctcgggttt ctatcccaag gtcgcggggt atgatccgtc cgcgcagagc ctggccctag
     2521 ctcggaaacg tcacgctcgt gccgagttct acgacagggt ggaagcgatc ccgaatcggg
     2581 ccttcgacgc c
//
LOCUS       DASTKU010001093         3281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_217190, whole genome shotgun sequence.
ACCESSION   DASTKU010001093 DASTKU010000000
VERSION     DASTKU010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217190"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VFQ61_39060"
     CDS             <1..185
                     /locus_tag="VFQ61_39060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960555.1"
                     /translation="GNSYGNSLRCVNSCEDGVLAGGLVMVGRVEPAQPGSASSASTEP
                     VSKAERWKNIVGRSPG"
     gene            complement(310..2790)
                     /locus_tag="VFQ61_39065"
     CDS             complement(310..2790)
                     /locus_tag="VFQ61_39065"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9960556.1"
                     /translation="MARPQPSTAFALDEDLWFGDGDDKEAESRAAASLAATGARIVGA
                     KPFPIAARRLDELTRNPNTRIEQVVAVLESDPALSARLLRLVNSAGYGLKVRCSSVRH
                     AAVLVGTRRLNQVATTAAILDLFDGSSGLGMELLEHAAFVGSLCRYLAVHLGLPHDEL
                     FTCGFLHDIGKLMLLDAERERYAELLQEFGGQADRIHIEERRVFGFDHAVLGAHVLSA
                     WNIPEPVPRVIAWHHAPQRALQDTVIAAMVETLRLSDLLAYVLPTHDAEEGARICGES
                     ESARYLDVSGVQLSAMWEDLSALRDRSRARSHGEPEMEAIVPKRESSDRSAPKTSMPA
                     VRTSQRADRPLHEVPTHFPCSVCAKPSYGNHCACCGGHVCPEHQVGLSEWCTTCVREF
                     KFFRKNHRIAWPVRLLAGVAVTGTLVVALFSAARAPEAGIEAWILAPFVVLALWSVVL
                     PVAFRLWQKLEFLEGRRKTTELERGPVARAQTPAPEVLPDSDPSVHVESRRNSEKTLA
                     SLRPAPELQDGDASSAPLSLAPPSSPPRAVRFSQAPRRPSLAPDGSPSSSPSPVLDVA
                     MARGSMPSAPMPRFSTLNFSQPPRAVTMNAASMPATATSMVPGAEVVPISAGNRASRR
                     PSLEPEPAPARVQVTIQSPAPPPAVDDDAPGIKAPVPERLVSSFPAALAEPIAVVERV
                     RSSVSPREVPPREIPPLDSREPAPREESDPMLRRESEPTLRAQLDREDPLLADIEPPV
                     SSTSEPVASSELFAEPQPSLRVGNTPLLLESAVSCKPPELRACSIPSPAIQQCAAPSV
                     PQRPPAPAPRQRAENGRSRRKNRRRRGR"
     gene            complement(2832..>3281)
                     /gene="fliW"
                     /locus_tag="VFQ61_39070"
     CDS             complement(2832..>3281)
                     /gene="fliW"
                     /locus_tag="VFQ61_39070"
                     /inference="COORDINATES: protein motif:HMM:NF014660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HET9960557.1"
                     /translation="QRFGALEVGEDQLVDFPEGVVGFPQERRFFLVPHNSTGFLAWLH
                     SATTPGLALPVVSAHAFGSRYPDVDLVQAAAAAGLGDDIEQLAVMVVLCAPPGQPATV
                     NLLAPIVINVATNRGAQVILEGSRFTTRELFVREVEAAAPKAQAAAE"
BASE COUNT          594 a         1078 c         1091 g          518 t
ORIGIN      
        1 gaggcaactc gtacggcaac tcgcttcgtt gcgtcaacag ctgtgaagac ggcgtgctgg
       61 caggtggctt ggtcatggtg ggacgggtcg agccagcgca gcctggaagc gcgagtagcg
      121 ccagcactga accggtcagc aaggcagaac gttggaaaaa catagttggg cgcagccctg
      181 gctgagcagg gacgcccaag gatgcttcca actgaaccgc ttggcgaggc ccgtccgccg
      241 gggccgagct ggacacggga tatgggaggg ctgcgggccg ttcggcccat tcctcggagc
      301 tccgcgcgtt cagcgcccgc gacggcgacg gttcttgcgt cgcgatcgcc cgttttcggc
      361 gcgttgcctc ggagcgggcg ccgggggccg ctggggaacg ctcggggcag cgcactgctg
      421 gatggcgggg ctcggaatcg agcacgccct cagttctggc ggcttgcagg atacggcgct
      481 ctccagtagc agcggcgtat ttccaacccg caagctcggt tggggctccg cgaagagctc
      541 agagctggct acaggctctg aagtcgaaga caccggcggt tcgatgtcgg cgagcaaggg
      601 gtcctcgcga tcgagctgtg cacgcaacgt cggttccgac tcgcgacgca gcatggggtc
      661 ggactcctcg cgcggcgcag gctcgcgcga gtcgagcggg ggaatctcgc gcgggggaac
      721 ctcgcgcggg gagacgctgg atcgcacccg ctccacaacc gcgatgggtt ccgcaagggc
      781 tgcggggaaa gacgagacca gccgctcggg cacgggcgct ttgattcccg gagcatcatc
      841 gtccaccgct ggtgggggtg cggggctctg gatcgtcacc tgaacccggg ctggcgcagg
      901 ttctggctcc aaagaaggcc gacgtgaagc gcggttgccc gcggagatag gcaccacctc
      961 ggcgccggga accatcgacg tcgccgtcgc cggcatggag gccgcgttca tggtcaccgc
     1021 tcgcggtggc tggctgaagt tcaaagtgct gaagcgcggc attggggcgc tcggcatcga
     1081 ccctcgcgcc atcgctacgt ccaaaaccgg agatgggctg ctgctcgggc taccgtcggg
     1141 cgccaaagat ggccgccgcg gagcctgcga gaagcgcacc gcgcgaggcg ggctggaggg
     1201 cggcgccagt gaaagtggtg ctgagctcgc atcaccatct tggagttcgg gcgcgggccg
     1261 caatgaagcc agcgtcttct ccgagttgcg acgcgattcc acgtgcacgg acggatcgga
     1321 gtccggtagt acctcggggg ccggcgtttg cgcccgcgcc accggtccac gctccaactc
     1381 cgtagtctta cgacgacctt ccaggaactc gagcttctgc cagagccgga aggcgacggg
     1441 caacacgacg ctccacagcg ccagcaccac gaaaggcgcc agaatccaag cttcgattcc
     1501 agcctcgggc gcccgggccg cgctgaacag agcaaccacc agagtgcccg tcaccgccac
     1561 gcccgcgagc agccgaacgg gccaggcgat ccggtgattc tttcggaaga acttgaattc
     1621 gcgaacgcag gtcgtgcacc actcgctgag ccctacctgg tgctcggggc acacgtgccc
     1681 gccgcagcaa gcgcaatgat tgccatagct cggcttggcg caaaccgagc acgggaagtg
     1741 ggtcggcacc tcgtgcagcg gtcgatccgc gcgttgcgag gtacgcacgg ccggcatgct
     1801 ggtcttgggg gcagagcgat ccgagctctc gcgcttcgga acaatcgcct ccatctccgg
     1861 ctcgccatgc gagcgagcgc ggctacgatc acgcagcgca gagaggtcct cccacatcgc
     1921 cgagagctgc acgcccgaga cgtccagata gcgcgccgac tcgctctccc cgcaaatccg
     1981 agcgccctct tcggcgtcgt gcgtgggcag cacgtaagcg agcagatccg agagccgcaa
     2041 ggtctccacc atcgcggcga tcacggtatc ttgcaacgca cgctgcggag cgtggtgcca
     2101 tgcgatcacg cgcggaaccg gttcggggat gttccacgcg gaaagcacgt gcgcgccgag
     2161 cacggcgtga tcgaaaccga acacgcgccg ttcttcgatg tgaatgcgat cggcttgccc
     2221 gccgaactct tgcagcagct ccgcgtagcg ctcgcgttcg gcgtcgagca gcatgagctt
     2281 gccgatgtcg tgcaagaagc cgcacgtgaa cagctcgtcg tgcggcagcc cgagatgcac
     2341 cgccaagtaa cggcacaatg aacctacgaa cgcggcgtgt tcgagcagct ccatacccaa
     2401 gccgctgctg ccatcgaaca ggtccaggat tgcggccgtg gtcgccacct gattcaggcg
     2461 ccgagtaccc acgagtacgg cggcgtgccg caccgagcta cagcgaacct tcaacccata
     2521 gccagccgaa ttcacgagcc gtagcaagcg cgcggaaagc gccgggtcgc tctccagcac
     2581 cgcgaccact tgctcgattc gggtgttcgg gttgcgcgtc agctcgtcca agcgccgggc
     2641 ggcgattgga aacggcttgg cacccacgat tcgggcgccg gtcgcagcca agctggctgc
     2701 agcgcggctc tccgcctctt tgtcatctcc gtcaccaaac cacagatctt cgtctaaggc
     2761 gaatgcggtg cttggttggg gcctggccac ggatcctctt ttcccgaagt aacggatgct
     2821 tgttccgctg cttattccgc tgcggcttga gctttgggtg ctgccgcttc gacctcgcgc
     2881 acgaacaact ctcgggtcgt gaagcgcgac ccctctaaga tgacctgcgc gccgcgattg
     2941 gtcgccacgt taatgacgat gggagccagc aaattgacag tcgccggctg ccccggagga
     3001 gcgcacagca ccaccatcac cgccaattgt tcgatatcgt cacccagccc cgccgcggcg
     3061 gcggcttgga cgagatcgac gtcggggtag cgacttccga acgcgtgcgc cgacaccacc
     3121 ggcagggcga gtcccggagt agtcgccgag tgcaaccacg ccaagaaccc ggtcgagttg
     3181 tgcggaacca gaaaaaaccg tcgttcctgc ggaaatccga ccactccctc ggggaagtcg
     3241 acgagctgat cctcgcccac ctctagcgca ccgaagcgct g
//
LOCUS       DASTKU010001094        19190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1779916, whole genome shotgun sequence.
ACCESSION   DASTKU010001094 DASTKU010000000
VERSION     DASTKU010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 19190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19190
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779916"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2482)
                     /locus_tag="VFQ61_39075"
     CDS             complement(<1..2482)
                     /locus_tag="VFQ61_39075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960558.1"
                     /translation="MRRAALICGTGVCLLSGLACTGTHGNPAPILGRVPHSVSGTSAS
                     PAAKAVREPDGAWGIPNFFELGSAVRTEQSEVAVDTTAWLQALSNRDERTPAAYLNAL
                     HAPRNKVLIYPEQDGLGRTRRFALVLPEQRTFSQNDAVRAYWGRSAEFLSFAVGAAHS
                     AGTVLMGPGEAARAVTLRSLLEPSGVNAQEFENSYQLEGTSRDVHEHKGLVPGLPVAF
                     AEFAFVERASHVREPGKELLPTTDPSGPVAQAGVSPALILTFAPLNHGVWRELSSASV
                     GRHRVLVMSNDEGEVYFVAAPLALAPSWHFGAMGRDLVAQAGPLPDPVRARDQPAAIL
                     LLPRATRARLFSGKVKSSAEIGGLLDQAEHFVDGAARARDQLDPGASSTVIRHYARAL
                     LETHRSRLDARLLTRFQYGDVEQPINASGGAGAIRFTSDQAAALRALAHSYRRTRDPE
                     LLTSLRGLALASLAAIGPGGTIVDRRFDQMALLGNSDPKTARADAQIDNGGVAVTFNH
                     RNLIFARGQDHLPGFTWGSLRLDSAANMLLDGETDEFAIDANSIPRTVDPAQSSLEIS
                     RWFSSKHVRARETARVIRGLPAARISYMLEPRTRHGSEPLSANVTLGDFLDYGSGNNE
                     AFQNRFGLSRVIGGAPLPVAFWMEGAEAPLWGDNSPSGLVDVTNEMKRLHPRFVAVYA
                     YDRAALFYFTKLPDQLFLRNVQGGMAGSYHGFTSLTARYEIPAANDGQRTELSPVYGY
                     TLWAPLYSEDDDGVPDQLQQLGKVWANTIEDHERGAGAVQGHSSLPEGDCCANPLKNA
                     FYLSLQSDTGYADLQYAWALVSDLLAEAA"
     gene            complement(2485..9528)
                     /locus_tag="VFQ61_39080"
     CDS             complement(2485..9528)
                     /locus_tag="VFQ61_39080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960559.1"
                     /translation="MSPPDALLEQIVSVARGELTHVYRVRLLADGTASMERYENGAFT
                     SPRIFTGAEHDGSFGGARAGDDGASPEKRLERAVRYAVTYLDGSVNVGERDLLSPTSA
                     LTRNVRWFPGDIQMLNTAFHYVLELFPSAQFHVTQLFRRVFGDIAFLALILLTSTFWL
                     RTQASTTAKQVALLLQVLSMVAIIGFDRFWEPIRYRVRKLSEVRWTGRGARVVIELPW
                     IVVSQVAAGVVGVTVNTLIGLTNLVINPFKIFLSWRQVGRGKTLEWKASSTSAGQDMR
                     GWPLAEFLDVYGPAARFGLYTLACLLVLVSLGAPLDLLGLNSFGVFLASFLTAWLYAW
                     YAALPRRIDDGAPQYQLAPREFWSLTIAGFAGLGLSAALFALGLYPAPAFEKSAGWVA
                     LFLTATSAFAALYPTYYRYKHRAYSISSERARSRLSRLNAALALVAVAVLAAALVLPR
                     PLLPEAGERLRDFVLADGARFRIPEVERRVYSTVAAAIKQTRNADPEPLLRSEGDQVT
                     LAQIRPTPSLDVAELQGIPEPRPPKLARLDSLRLPEVIGLPVGARIPTNKPRREAVLP
                     SAAPNKPLSQPRAASQQTVQEARERFRLAEARKLASGEQAAQRAFLDAASYPWISREE
                     LERLGAIDERGAGVALPELARVYRFEDVKALWDRVPAVTSRKLSPRARFTMLLEGVDG
                     AVSLGLDPSAENVQRFLATESEIAERWPIDYPNVPLTSLPGRATEAVSRVFGFARLAL
                     QSELSPRELASRWRLDYVNTHWNTAAIGPTLREVYRKRPTTDPWDNFQLDFGGNDELR
                     ARWEGLQTEQVVASAALGPSAAQLPVEHLTLAAKLLNAAAAASDSPPGDSTTPAGSDL
                     VQAALQELAVPEGTDRVRLASGDATASALSARLRTQILSARIGELAKAVAGGRALPGS
                     TEDHQLLLEAAELRFGAQGASAEQRTLLQWLLLIDASETYKEIAAGYDRYQRENAIRG
                     IKSDALARAPELDDEQVWRDLMYLWRELPQRFPHLPIRADLVGEFVCQTAYLSSRNAR
                     DPRGTHEFIEDFSDVFESVDALMATAPPAALQRFADVSFEKTQGRTSKSAQARRFFAW
                     WQAAGLARAVVYNRQQHGAAKVNPVKTSPLATPAKEVEEVARDWAGILEGGRVRYPHL
                     PWDSAGFSEFFTTILELEGWTLPELWQHFDGKLARAERMIAAHVAAFQSFEELVDRRI
                     AEKTNAPSLDRRLREANAVLALIDLLSALESNGVRSYTNNPTRLSRDFSALYDSLAKR
                     YPNLYWPAEGAVEAYFLRQVIEGWDEYETREHFAPELALADTLAAHGLRERFERIASE
                     DASRLSAADRALRTFIDVQAQRVEKKSGSRITQPRARAFNALVATATLLMSAAQEGLL
                     ALSPSSGAPVEKTPSHASVQAWARRVSGTAQTRTLVPFAESLVRDFGELHAAMRGEGP
                     AFPWEDGAVLETELFVLRKSGKELQSALPALSPTWKTASDYIRRQVSLPESFTQLVTR
                     DAREELRSKLARELGVTAGEIPDPELARREDPEVFGLNALLVLSNIASQIHVAYHEEP
                     NPSELAQALVEIRREGPKRYPHLPWAAKGFVPSLIIGAWRPELARDFWRYAALIDLAS
                     ADTLLAEASKLSSNPSVPAPGCEGSKQAIPCEVYADMRTIMREQTGRTPASDEVVAFQ
                     ILKDASALLKEFFPAVPVNFQSALALARVRTRLRSLDSEFPQLHILKTDDQTTRVGYA
                     ERLTAISAKLAIEAGISLADPRFDELAVHKLQDKYLLSMSRIYDLVRHSFLPGELEYF
                     AQSIVAESVEENAKRAARHGLPASALSVPEVKSEDVVSDFALYELLYAEEIGQDRDYV
                     VSLLTIFDQLRSRPDIRAAHERAMRDIDRAFEGDMRQPTHPDYTAWLASEAHREWQSQ
                     RGWLYKRSRGKLLALARSFALLQREAFSDRSAPDARAAFARFGSFNQFMTRFFDNSAA
                     VHRVPELHAALQRLEQEDAFLADGVEFAIAIFKSHVLDAIYPGAERSFAALAGISRQL
                     PQVQADYRHLLGFTPGLESGVPFYDSFDSWLRRRVEHGDGTDLFRRLNMVQRSLQSWP
                     NSFVLKRAYKVLFDRDLDEEDPTPVARSGKYDLLPKTVGSEVKDFLESRLPEALLRET
                     GSRDPAAWLAWLMQHGEIELDGSRASTNAYLDTVQGYQQRLAEYQKRIEQNGARGRPA
                     RAELQRHLDIEREYRSFRAQVEALERTARRVPSLVSAAGRDYTLAIRQATVWIALLVL
                     SLVLGWRWSSKRLLGFVMRKGALALTLSLAAVPLAVALSPAHSAEFAGGTLRAIGLLG
                     PATCAGAELPCVAPRGAAAQLLKIRPWTPLLGGQGSQGGFE"
     gene            complement(9577..12438)
                     /locus_tag="VFQ61_39085"
     CDS             complement(9577..12438)
                     /locus_tag="VFQ61_39085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960560.1"
                     /translation="MLPSESRIATTGSQFDPHPRWESNWGGPVEGPVRGGLASRERQP
                     FWSRQWSRYGARIVAVTGLGGVAWGATVVAHGAAMRPVLFALFGTLVIAVGALARAGN
                     RPALLLAGLFGVPLNLPGFRGDPRSSIRNKASWLAQFMQERPRGFNTQGAHLVYGAVA
                     VKRLLKTICVREEKDSSVLKDLLRRRTAVVAELGECSAEVSRTLAAGEAGAAAASRRE
                     FTLSQELEQLDARILRARLDIQRRVVAEFHAAWLELQRSGVIDARTHGEVAQQFRQSP
                     GQAEALLPNGLIPGPTAYVFEYGMFAGRCALACLFVATFDTPYLLVALGLLVLNIYVI
                     EPVVQKLYISTFNLLLASPGHKLVDLKRYVETHVPAGRVLRVPIIVPKFSSNPALNNL
                     QAIIAAAARRSGLEPVRVVPFAIRDGRRDTVVEFASNEVPPPAPARLLAFRSALESEF
                     DRQRAKFPLNVRVTAESNQLRIELLDDENVIWDLVGEDASQAFIYLRRNLTALRDTLT
                     HVGPKLQPVFLFVSNTKDPDAIAYEVKHVAELQERSDREFGGQVGFLHLLRGGAWYNF
                     NAYLQRFDASDKDFQRALPEFRRRFAEHEFAAKEYLLERLADGRSADDFARAFNLALR
                     DPAFYEQFASFDWDTLPSELFPTAATFALLERARSGERLRDGELCALNRELLLTVVPA
                     RIRGDFFKKVGNDIAVHELIVAGQTKPTIYVSRASGEHVQDPHQPNFVRVWGDFARYT
                     GLHGTNEQIQRAILAGEELIVDHVPEVAAIVDDKNEFGPGELEKGISIFLHPENRHIV
                     IGVPRIDITLPEVRGCAMASEYILSARLARNCHNHSDGRSRAAVYSSSSAAYGKWLHR
                     PTEYLAHYAREVLNPAHALSHDFQQSYLVAGAAGCLAGFSEALYGPTRFHARVDSLVP
                     KGRVQPEPHGPRKSKPLAALLPARAESVMTSIGQQHTPN"
     gene            complement(13560..13859)
                     /locus_tag="VFQ61_39090"
     CDS             complement(13560..13859)
                     /locus_tag="VFQ61_39090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960561.1"
                     /translation="MTLTITGLLILLVIAGICGAIGRAIAGGTSGGFFVSIAVGFVGA
                     LLGSVIANYMHLPELLTIAIEGRAFPILWSIIGAAVFVALIHLLSGGVSRRYRYY"
     gene            complement(14317..15570)
                     /locus_tag="VFQ61_39095"
     CDS             complement(14317..15570)
                     /locus_tag="VFQ61_39095"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002772.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET9960562.1"
                     /translation="MENVIAVVLGGGRGTRLFPLTQFRSKPAVPIAASYRLVDVAVSN
                     CIHADLRRIFVVTQYQSESLNRHIAHTYKFDAFQSGFIDILAAEQTDDGNDWFRGTAD
                     AIRKCKKHFGRESWDAIAILSGDQLYRMNLRDMLRVHRENRAAVTVAMKPVPADQTSG
                     FGIMKVDGSGRVTHFEEKPHASRLGDLGSSVPGVTGPAYLASMGIYVFERAELEKALA
                     DPSHIDFGKHILPMMLDRSRVQAFFYDGYWEDVGTIRSYYEANLALTSQTPTFDFYHP
                     EYPIYSERAFLAPTRMHGCQVTDSLIADGCILQEAQIERSVIGVRTRVGRGVKIRNSL
                     LLGADDYETHSEGIARSGSVIPLGIGEGSTIENAIIDKNARVGRRVMIRNERSLTQHD
                     GGNHFIREGIVIVPKGAVVPDGTVI"
     gene            16066..16326
                     /locus_tag="VFQ61_39100"
     CDS             16066..16326
                     /locus_tag="VFQ61_39100"
                     /inference="COORDINATES: protein motif:HMM:NF022716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HET9960563.1"
                     /translation="MTQRHTTEEHHPTNPKKPGPGDTGNLKKDPDDWVTGDEPMTQAQ
                     ASYLKTLSEEADVPFEDELSKAEASKRIEELQQRTGRGQGSG"
     gene            16505..17698
                     /locus_tag="VFQ61_39105"
     CDS             16505..17698
                     /locus_tag="VFQ61_39105"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HET9960564.1"
                     /translation="MKTANSSSEPADSCEPEGRRPSLFSAAIELTGACNQKCAYCYNE
                     WRGDSGASISTGGRDKVLARIRRLLDTFDVRELTLTGGEPFTRPDLFEILDLIRSRGV
                     AAHIISNGGLIDEERARALAEREVRFVQVTLNGAERELHEEHVGLGHFDATLEGIRAL
                     RAADVEVVGCIVVTRKNANHVADILELFDSLRVRHIALSRFSPAGYATRHVADLLPSR
                     ADLIAALSRAAPFVKARGMSIRVTMPIPPCAVEIEQFPDLIFGVCPVGTPQQEFAIGP
                     DGALRHCTLHREKLGGGLDIADPSLDLVKLVRGSEVSQYRATVPEFCRGCLHEKTCAG
                     GCGAAAEWVLGSRNHPDPIVAQHVDEAFGSELERLREPQRVRLNVLHASDDGCRHGAA
                     DYSGA"
     gene            17700..18248
                     /locus_tag="VFQ61_39110"
     CDS             17700..18248
                     /locus_tag="VFQ61_39110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960565.1"
                     /translation="MDDDSKKIILARRARFIAAALASVGLAGQACGGKSDGDGQAMAG
                     GGGASTGGTRSSTGGAPQPCLSIATGGSSFGGAINTGGTSSAGGVASAGAVGTGTSFG
                     GSSGGATPQPCLQPPIELPPEGSGGRTGAAGSTGTAGSAGSAGQAGGAEDGGTQNEGG
                     GANEGGLFGSGGVPQPCLAPPR"
     gene            18275..>19190
                     /locus_tag="VFQ61_39115"
     CDS             18275..>19190
                     /locus_tag="VFQ61_39115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HET9960566.1"
                     /translation="MKRSIDRLRVLLIWPGGTLGALSNFGVPQLLSLASAIRARCDVE
                     LSIADLDCERALGPLDLSRLCQGHDLIGISCYSSFDYLKVMALAARIRELSPRAILVT
                     GGYHPSACPDDFTQPGSPFDYVAVGDGEAPLARLIEQLSRGRRPLNRILPSEPVPSPS
                     ELLPYDFELLARYLPIARRVATQAEIYLSRGCPYDCSFCMERAKRETSWRAVSPEEAI
                     DTLHQLDRFLDLQGWTLFIADALFGMKRAWRREFLEALARKPLRALRVWLLIRVDLIE
                     REDLELMSRAHVSPGFGLESGDPNQLKRI"
BASE COUNT         3635 a         5955 c         6053 g         3547 t
ORIGIN      
        1 gcgccgcctc ggcgagcaag tcgctgacta acgcccaagc gtactgaagg tcggcgtagc
       61 cggtatccga ttgcagtgac aagtagaaag cgttcttcag agggttcgcg cagcagtcgc
      121 cctccggcaa cgacgagtgg ccctgaaccg cgcccgcgcc gcgctcgtgg tcttcgatgg
      181 tgttcgccca aaccttgccg agctgttgca gctggtctgg taccccgtca tcgtcttcgg
      241 agtagagcgg cgcccacaac gtatagccgt acacagggct caactcggtg cgctgtccgt
      301 cattcgcagc cggaatctcg taacgtgcgg ttaggctcgt gaagccatga taacttccgg
      361 ccatccctcc ctgtacgttc cgcaagaaca actgatctgg cagtttcgtg aaatagaaca
      421 gcgccgctcg atcataggca tatactgcta cgaagcgggg gtgaagacgc ttcatctcgt
      481 tcgtcacgtc gaccaatccg gaaggtgaat tgtcgcccca caatggcgct tcggcaccct
      541 ccatccagaa tgcaactggc aacggcgctc cgccgatcac ccgactcaag ccaaagcggt
      601 tttggaatgc ctcgttgttg cccgaaccgt agtccaagaa gtcacccagc gtcacgttcg
      661 cactcagcgg ttccgagcca tggcgtgttc gcggctcgag catgtagctg atacgtgcgg
      721 caggcagacc ccgaatgacc cttgccgtct cgcgcgcacg tacatgcttc gacgagaacc
      781 atctcgatat ctccaggctg gattgcgctg gatcgaccgt tcgcggaatc gaattggcgt
      841 cgatcgcaaa ctcgtccgtc tcgccgtcta ggagcatatt ggcagcagaa tcgagccgga
      901 gcgagcccca ggtgaacccg ggcaagtggt cctgaccacg cgcgaagatc aggttacggt
      961 gattgaaggt gaccgcgact cctccattgt cgatctgagc gtcggcgcgc gcggtcttcg
     1021 ggtcgctgtt gccgagcagc gccatttggt cgaagcgtcg atccacgatc gtgccgccgg
     1081 ggccgatcgc ggcgaggctc gccagggcta ggccgcgcaa gctcgtgagc agctcgggat
     1141 cacgggtgcg ccgatacgag tgggccagcg cacgcaacgc ggccgcctga tcgctcgtga
     1201 agcgaatggc gcccgcgccg ccagaggcgt tgatgggttg ttccacgtct ccgtattgaa
     1261 atcgagtgag aagccgcgca tccagccggc tgcgatgtgt ttcgagcaga gcacgcgcgt
     1321 agtgtcgaat caccgtgcta ctcgcccctg ggtcgagctg atcgcgtgcg cgggcggcgc
     1381 catccacgaa atgttcggcc tgatcgagga ggcctccaat ttcggcgctg ctcttgacct
     1441 tgcccgagaa caggcgtgcg cgcgtcgctc gcggcagtag caagattgct gcgggctggt
     1501 ctcgcgcgcg gactggatct gggagtggac ccgcctgcgc gaccaagtct cggcccattg
     1561 caccgaaatg ccatgacggc gcgagcgcca gcggggcggc cacgaagtac acttcgccct
     1621 cgtcgttaga catgaccagc acgcggtgac ggccgacgct cgcactactc agctcacgcc
     1681 aaacgccgtg attcagcggc gcgaacgtca gaatcagcgc gggtgataca cccgcctgag
     1741 cgaccgggcc gctcggatcg gtcgtgggga ggagttcttt cccgggctca cgcacgtgcg
     1801 aggcccgctc gacgaaggcg aattccgcga aggcgaccgg caaccccggc accagacctt
     1861 tatgctcgtg cacatctcgt gaggtaccct cgagttgata tgagttctcg aactcctgcg
     1921 cattgactcc cgacggctcg agcagcgatc gcaacgtcac cgcccgcgcg gcctcgcccg
     1981 ggcccatcaa tacagtgcca gcgctgtgtg cggccccaac ggcgaagctt agaaattcgg
     2041 cgctccgccc ccagtacgcc ctcaccgcgt cgttctgtga aaaggtgcgc tgctccggca
     2101 gtacgagcgc gaaccggcgc gtgcgtccga ggccatcctg ctccggatag atcaggactt
     2161 tgttgcgggg tgcatggagc gcattcaggt acgccgcggg cgtccgttca tcgcggttcg
     2221 acagtgcctg aagccacgcc gtggtatcga cggcaacctc actttgctcg gtccgaaccg
     2281 cgctcccgag ctcgaagaag tttgggatgc cccaagcgcc atcgggctcg cgaacggctt
     2341 ttgcggcagg ggaggcactc gttcctgata ctgaatgggg gacgcggccg agaatcggcg
     2401 cggggttgcc gtgggttccc gtacaagcca agcctgagag caaacacact cctgtgccgc
     2461 agatgagtgc ggctcgacgc atgatcactc gaatcctcct tggctgccct ggccaccgag
     2521 cagcggtgtc caaggtcgta tcttgaggag ctgcgcggcc gcgccgcgcg gggcgacgca
     2581 gggcagctcg gcccccgcgc aagtcgcagg gcccaaaagc ccgatggccc gtaatgtccc
     2641 acccgcaaac tcggcggagt gagccgggga aagcgccacc gcgagcggaa ccgccgcgag
     2701 cgatagcgtc aaagccaacg cccccttccg cattacgaag ccgagcaggc gcttcgaact
     2761 ccagcgccac cccagcacca gactcagcac cagcagcgca atccacaccg tcgcttgtcg
     2821 aatcgcgagc gtgtagtcgc gccccgccgc ggagaccaag ctcgggactc gacgcgcggt
     2881 ccgctccaga gcttcaacct gggctcggaa cgaacgatac tcgcgttcga tatcgaggtg
     2941 ccgctgcagc tccgctcttg cgggccgccc acgcgccccg ttctgctcga tacgtttctg
     3001 atactccgcc agccgttgct ggtagccctg gaccgtatcc aagtaggcat tggtcgaggc
     3061 gcggctgcca tctagctcga tctcaccgtg ttgcatcagc caagccagcc aggctgccgg
     3121 atctcgtgag cccgtctccc gcagcagcgc ctccggcaaa cgcgactcga ggaaatcctt
     3181 tacctcggag cctacggtct tcggcagtag atcgtacttg ccggaccgag ctacgggcgt
     3241 cggatcctct tcatccagat cacggtcgaa gagcaccttg taagcacgct tcagtacgaa
     3301 ggagttcggc cagctctgga gactgcgctg gaccatattc aggcgacgaa acaagtccgt
     3361 tccgtcgcca tgctccacgc ggcgtctcag ccacgaatcg aaggaatcgt aaaagggaac
     3421 cccagattcg agcccgggcg tgaatccaag gaggtggcga tagtcggcct gcacctgcgg
     3481 caattgcctg ctgatgccgg cgagggcggc aaagctccgc tccgcgcccg gatagatcgc
     3541 gtctagcacg tgtgatttga agatcgcgat ggcgaactcc actccgtcgg cgagaaaagc
     3601 atcctcttgc tccagacgct ggagggctgc gtgcagctcc ggcactcgat gcacggcggc
     3661 gctgttgtcg aagaagcgag tcatgaactg attgaagctg ccgaaacgag cgaaggcggc
     3721 gcgtgcgtcg ggcgcgctcc gatcggaaaa ggcctcgcgc tggagcagcg cgaacgaccg
     3781 agccaacgcc agcaacttac cgcgagacct cttgtacagc caaccccgtt ggctctgcca
     3841 ttcccggtgt gcttccgagg caagccaagc ggtgtagtcg gggtgcgtgg gttgacgcat
     3901 gtcgccctcg aacgcccggt cgatgtcccg cattgcccgc tcgtgcgccg cacggatatc
     3961 gggccgactc cgcagttgat cgaagatcgt gagtaacgag acgacgtagt cacgatcttg
     4021 tccgatttct tccgcgtaga gcagctcata cagcgcgaaa tcagacacga cgtcttctga
     4081 cttgacctcg ggtacgctga gcgcgcttgc gggcagcccg tggcgcgccg cccttttggc
     4141 gttctcctcg acactctcgg cgacgatgct ttgcgcgaag tactcgagtt ctccgggcaa
     4201 gaaactgtgg cgcacgaggt cgtagatccg cgacatggag agcaaatact tgtcttggag
     4261 cttatgtacc gctagctcat cgaaacgcgg gtcagccagg cttatccccg cttcaatcgc
     4321 cagcttcgcc gaaatcgcgg tgagacgctc cgcgtagcca acgcgcgtcg tttggtcgtc
     4381 tgtcttcagg atgtgaagct gcgggaattc gctatcgagt gagcgcaggc gagtacgtac
     4441 gcgagccaat gccaaggcac tttggaaatt gaccggcacg gcggggaaga actccttcag
     4501 taaggcactc gcatccttga ggatttgaaa ggccaccacc tcgtccgacg ctggcgttcg
     4561 ccccgtctgt tcgcgcatga tcgttctcat gtcggcgtag acctcgcaag gaatagcctg
     4621 ctttgatccc tcgcagccgg gcgccggaac actcggattg gaggaaagct tgctcgcctc
     4681 cgccagcaga gtgtcagcgc tagcgagatc gatcaacgcc gcgtatcgcc agaagtcgcg
     4741 agccagctcg ggacgccagg cgccgatgat cagcgacggc acgaatccct tcgcggccca
     4801 tggcaagtgg ggatagcgct tggggccttc gcgtcggatc tcgaccaacg cctgagccaa
     4861 ctcggatgga ttgggctcct cgtgatatgc gacgtggatc tgagacgcga tgttcgaaag
     4921 aacgagcagc gcattcagac cgaacacctc gggatcttcg cgacgcgcga gctccgggtc
     4981 ggggatctcg ccggctgtca ctccgagttc acgcgctaac ttggatcgca gctcctcgcg
     5041 ggcgtcgcgg gtgacgagct gggtgaatga ttcgggaagc gacacttgcc gacggatgta
     5101 gtcgctcgcc gtcttccaag tcgggctgag ggcgggcaaa gcgctctgca gctccttacc
     5161 ggatttacgc aacacgaata gctcggtctc gagcaccgcg ccgtcttccc aggggaacgc
     5221 cggcccttcg ccgcgcatgg ccgcgtgcaa ttcgccaaaa tcacgcacca ggctttccgc
     5281 gaacggcacc agggttcgag tctgcgcggt accgctgaca cgacgagccc acgcttgcac
     5341 ggaagcgtga ctcggtgtct tctccaccgg cgctccactc gaaggcgata gcgcgagcag
     5401 accttcctgg gccgcgctca tcagcaacgt ggccgtcgcg acgagtgcgt tgaaggcgcg
     5461 cgcgcggggc tgagtgatgc gtgaaccgga cttcttctcc acgcgctgcg cctgaacgtc
     5521 gatgaacgtg cgtagcgccc tatcggcggc ggaaagtcga ctggcgtcct ccgatgcaat
     5581 gcgttcgaag cgttcacgca gaccatgggc ggcgagggtg tcggccagcg caagctcggg
     5641 cgcaaagtgc tctcgagtct cgtactcgtc ccagccctcg atgacctggc gcaggaagta
     5701 ggcctccacg gctccttccg ccggccagta tagattcgga tatcttttcg cgagcgagtc
     5761 gtacaaggcc gagaagtccc ggctcaaacg tgtagggttg ttggtatatg agcgcacgcc
     5821 gttgctctcc agcgcgctga gcaggtcgat tagcgccagc acggcgttgg cttcgcgcag
     5881 ccggcgatcg aggctcggcg cgttcgtctt ctccgcgatg cggcggtcca ccaactcttc
     5941 gaacgactgg aacgctgcga cgtgagccgc gatcattcgc tcggcacgcg cgagcttccc
     6001 atcgaaatgt tgccaaagct ccggcagcgt ccagccctcc agctcgagaa tggtcgtaaa
     6061 gaactcggag aaacccgcgg agtcccaggg caggtgtgga taacggacgc gtccgccctc
     6121 cagaatgccc gcccaatcgc gcgccacttc ctccacctcc ttggcggggg tcgcgagtgg
     6181 cgatgttttc accgggttga ccttggcagc tccgtgttgc tggcgattgt agacgaccgc
     6241 gcgggccagc cctgcggctt gccaccaggc aaagaatctt cgcgcctgtg cgctcttgct
     6301 ggtgcggccc tgggtcttct cgaagctcac atcggcgaac cgctgcaggg ctgccggtgg
     6361 cgcggtggcc atgagggcgt ccacgctttc gaatacgtcc gagaagtcct cgatgaactc
     6421 gtgggtacca cgcggatcgc gagcgttgcg cgaggagaga taggccgttt ggcacacgaa
     6481 ctcacccacg agatcggccc gaatcggcag gtgcgggaag cgttgcggca gctcgcgcca
     6541 caggtacatc aagtctctcc agacctgttc gtcatcgagc tcgggtgcgc gcgccagcgc
     6601 gtcgctcttg atgccgcgaa ttgcgttctc acgctgataa cggtcgtagc cagcggcgat
     6661 ttccttgtac gtttcactcg cgtcgattag cagcaaccac tgcagtaatg ttcgttgctc
     6721 cgcgctcgcg ccttgggcgc caaatcggag ctccgcggct tcgagcagaa gctggtgatc
     6781 ttcggtggag cccggcaggg ctctgccgcc cgctaccgcc ttggcgagtt ccccgatgcg
     6841 cgccgacagt atctgtgtac gcaggcgggc gcttagcgcg gaagcggtcg cgtcgccact
     6901 cgcgaggcga actcgatccg taccctcggg taccgcgagc tcctgaagcg cggcttgcac
     6961 cagatcagac cccgcgggcg tcgtcgagtc acccggcggc gagtcgctgg cagccgcggc
     7021 tgcgttcagt agcttggcgg ccaaagtcaa gtgctcgacg ggcagctggg ctgcgctcgg
     7081 tccaagcgcg gcactggcca cgacctgctc tgtctgcaat ccctcccaac gcgcgcgcag
     7141 ctcgtcatta ccaccgaagt cgagctggaa gttgtcccac ggatccgtcg tgggtcgctt
     7201 gcgatagacc tcacgcagag tgggcccaat cgcggcggtg ttccaatgcg tgttcacgta
     7261 gtcgagtcgc catcgcgagg cgagctcccg cggagaaagc tcgctctgga gcgccaagcg
     7321 ggcgaacccg aacacacgac tgaccgcctc agtggctcgg cccggcaacg acgtcagcgg
     7381 cacgttcgga tagtcaatcg gccaacgctc cgcgatctcc gactcagtcg cgagaaagcg
     7441 ctgcacgttc tcggcgctgg gatcgaggcc cagacttacg gcgccgtcga cgccctcgag
     7501 caacatcgtg aaccgggctc gcggcgagag tttgcgcgac gtgacggcgg gaacgcgatc
     7561 ccacagggcc ttcacgtcct cgaagcggta gacgcgggcc agctcgggta gcgctactcc
     7621 tgcacctcgt tcatcgattg cgccgagccg ttctagctct tcccgggata tccacgggta
     7681 gctagcggcg tcgaggaacg cgcgttgagc ggcctgctcg ccgctggcga gttttctcgc
     7741 ctccgccaaa cgaaagcgtt cccgagcctc ctgaacggtt tgctgacttg cggctcgcgg
     7801 ttgagagaga ggcttgttcg gcgccgcgct tggcagcact gcctcccggc gcggcttgtt
     7861 cgtggggata cgtgctccca ccggcaagcc gatgacttca ggcaagcgca gtgaatcgag
     7921 acgcgcgagt ttcggaggtc gtggctcggg aatgccctgc aactcagcaa catccaagct
     7981 cggagtcggt cggatctgcg cgagcgtgac ttggtcaccc tcggagcgca gcaacggctc
     8041 gggatcggcg tttcgggttt gtttgatggc cgcggcgacc gtggagtaca cgcgccgctc
     8101 gacttcgggg attctaaagc gcgccccgtc cgccagcacg aagtcacgca ggcgttcacc
     8161 cgcctcgggt agcagcggcc ggggaaggac cagagccgct gcgagcacgg ctacggcgac
     8221 cagcgcgagt gcggcgttta gccgactcag ccgcgagcgg gctcgctcac tcgatatcga
     8281 gtacgcgcga tgcttgtatc gatagtaagt aggatagagc gccgcgaaag cagaagtggc
     8341 cgtcaggaac agtgcgaccc agcccgctga cttctcgaat gcgggcgcgg gatacaaacc
     8401 caacgcgaac aacgcggcgg agagccccaa gcccgcgaag cccgcgatgg ttagactcca
     8461 gaactcccgg ggggcgagct ggtattgagg ggccccatcg tcgatgcggc ggggcaacgc
     8521 cgcataccag gcgtagagcc acgcggtcag aaaggaggca agaaacaccc cgaagctatt
     8581 tagtcccagt aagtcgagcg gggccccgag gctgaccaaa accagaagac acgccagagt
     8641 gtacaggccg aatcgcgcag caggaccata gacgtccagg aactccgcca acggccagcc
     8701 tcgcatgtct tgtcctgcgc tggtcgacga cgccttccac tccagtgttt tgccgcgacc
     8761 cacctgccgc caagaaagga aaatcttgaa cggattgatg acgaggttag tgagaccgat
     8821 cagggtgttg acggttactc ccactacccc ggccgcgact tgcgagacga cgatccacgg
     8881 tagctcgatc accactcgcg cgccgcgacc agtccagcgc acctcactca gctttcgcac
     8941 gcgatatcta atcggctccc aaaatcgatc gaagccgatg attgccacca tggacaagac
     9001 ttgcagcagt aaagccacct gtttggccgt ggtggaagcc tgcgttctga gccagaaggt
     9061 actcgtgagt aagattagtg ctagaaacgc aatgtcaccg aagacgcgac ggaagagctg
     9121 ggtgacgtgg aactgcgctg acgggaacag ctccaacaca taatggaatg ccgtgttcag
     9181 catctggatg tcgcccggaa accaacgcac gttgcgagtc aacgcgctgg tgggcgacaa
     9241 caagtctcgt tcaccgacat tcacacttcc gtcgagatac gtgaccgcgt aacggacggc
     9301 acgctccaac cgtttctcgg gcgacgctcc gtcatcgccc gcgcgggctc ccccgaaact
     9361 gccgtcatgc tcggcaccag tgaagattcg ggggctcgtg aacgcgccat tctcgtagcg
     9421 ctccatgctc gccgtgccgt ccgcgagcag gcgtacgcgg taaacatggg tgagttcgcc
     9481 acgggctacg gagacgattt gttcgagcaa cgcatctggt ggagacattc ggatcagctg
     9541 ttcgggcacg ggccgggcga cgtatcgaaa tgaacgttag ttcggagtgt gctgctgccc
     9601 gatactcgtc atcacactct cggctcgagc cggcaaaagc gcggccagtg gcttcgactt
     9661 cctgggcccg tgcggctctg gctgcactcg acccttgggt actagtgaat ccactcgtgc
     9721 gtggaagcgc gtgggaccgt acagcgcctc ggagaaaccg gcaaggcaac ctgcggctcc
     9781 ggccaccaaa taggactgct ggaaatcgtg gctcagcgcg tgcgccggat tgagcacctc
     9841 tcgcgcgtag tgcgccagat actcggtcgg ccgatgcagc cacttgccgt aagcggccga
     9901 tgacgagctg taaacggcgg cgcggctgcg gccatccgaa tgattgtgac aattgcgagc
     9961 caggcgggcg ctcaagatgt actccgaagc cattgcgcaa ccgcggactt ccggcagcgt
    10021 gatgtcgata cgcggcacgc caattacgat gtggcgattc tcgggatgca ggaagatcga
    10081 gattcccttt tccaactcac cggggccaaa ctcgttcttg tcatcgacaa tcgccgcgac
    10141 ctcgggcacg tgatctacaa tcagctcctc accggccaga atcgcgcgtt ggatctgctc
    10201 gttagtgcca tgcagcccag tgtagcgggc gaaatcgccc catacccgga cgaagttcgg
    10261 ctgatggggg tcctgtacgt gttcgccgct ggcgcgcgag acgtagattg tcggcttggt
    10321 ttgacccgcg acgatgagct cgtgcactgc gatgtcattc cctaccttct tgaagaagtc
    10381 accgcggatt cgggccggaa ccaccgtgag cagaagctcg cgattcaagg cgcatagctc
    10441 gccgtcccgc aaacgttccc cgctccgggc ccgctcgagc agcgcaaacg tggccgcggt
    10501 ggggaacagc tcacttggca gtgtatccca atcgaaactc gcgaactgct cgtaaaaggc
    10561 gggatctcgc agcgccaggt tgaacgcacg ggcaaagtcg tcggccgagc gcccatccgc
    10621 gagccgctcg agcagatact ccttggccgc gaactcgtgc tcggcaaagc gtcgcctgaa
    10681 ctcggggagc gcgcgctgaa agtccttgtc gctcgcgtcg aagcgctgca ggtaggcgtt
    10741 gaagttgtac caagctccgc cccgcaacaa gtgcaggaag cccacctggc cgccgaactc
    10801 ccgatcggag cgctcttgta gctcggcgac gtgcttgacc tcgtaagcaa tggcgtccgg
    10861 atctttggtg ttgctcacga acagaaaaac cggctgcagc ttaggcccga cgtgggtgag
    10921 agtgtcgcgc agcgcagtca agttacggcg caagtagatg aaggcctggc tcgcgtcctc
    10981 tccgaccaga tcccagatca cattctcgtc gtcgagcagc tcgatacgaa gttgattcga
    11041 ctcagccgtc acgcggacat tcagagggaa cttcgcgcgc tggcggtcga attcactctc
    11101 gagggccgaa cggaacgcga gcaggcgtgc cggagccggg ggcggaacct cgttcgaagc
    11161 gaactccact acggtatccc gtcggccatc gcgaatcgcg aacggcacca ctctcacggg
    11221 ctcgagccca ctgcgtcggg cggcggcagc gataatcgcc tggagattgt tgagcgccgg
    11281 attggaggag aatttgggaa cgatgatcgg cactcgtaac acccgtccgg ctggcacgtg
    11341 agtctcgacg tagcgtttga gatctacgag tttgtgaccc gggctcgcga gcagcaggtt
    11401 gaaagtgctg atgtacagct tctgaaccac cggctcgatc acatagatat tgagaacgag
    11461 taagcccaac gccaccagca gataaggcgt gtcgaaggtc gcgacgaaca agcacgccag
    11521 cgcgcaacgg ccggcgaaca taccgtactc gaacacgtag gccgtgggcc cggggattag
    11581 cccgttgggc agcaatgcct cggcctggcc gggcgactgt cggaactgct gcgccacctc
    11641 accatgcgtg cgcgcatcga tcacgcctga gcgctgaagc tccagccagg ccgcgtgaaa
    11701 ctcggccacc acccggcgct ggatatcgag tcgggcgcgc aagattctag cgtcgagctg
    11761 ctccaactcc tgcgatagcg tgaattcgcg ccggctcgcc gcggcggctc ccgcttcgcc
    11821 cgcggcgagg gtccgggaca cctcagcgct gcattccccg agctcggcca ccaccgccgt
    11881 acgacgtcgc aacaggtcct ttagaaccga cgagtccttc tcttcccgga cacagatggt
    11941 cttcaataag cgcttcaccg ccacggcacc gtagacgagg tgcgcgcctt gggtgttgaa
    12001 gccgcgtggc cgctcttgca tgaactgagc tagccagctc gctttgtttc gaatcgagct
    12061 gcgggggtct ccacgaaacc cgggcaggtt cagtggaacg ccgaacaagc ctgcgagcag
    12121 cagggccggc cggttgccgg cacgcgcgag ggcgcccacc gcaattacga gcgtgccgaa
    12181 cagcgcgaag agcaccgggc gcattgccgc tccatgcgcc accacggttg cgccccaggc
    12241 gacgccaccg aggcccgtaa ctgcgacgat acgtgcaccg taacgtgacc attgtcgcga
    12301 ccagaagggt tggcgctcgc gggacgcgag gccacctcgc acggggcctt cgaccgggcc
    12361 accccagttc gattcccaac gcgggtgagg gtcgaattgg gagccggtcg ttgcgatcct
    12421 tgattcgctc ggtaacatcg atattcgctc cacggaatcg caggttcgtt gccgcccctg
    12481 cgggctccgt tctcgcttgt gagccgttcg tctttggaac tggctcgcgc tcaaagtgct
    12541 attcgctaaa tgagagcccg tcccactggg agctaatcgt cgcaggcgtc tgcggcgctc
    12601 ctacggaacg ggatctaagc tgctgagtca acgtgtgctc ttcgaatggt cgggtctagt
    12661 gcttgggttc ggtgcccttc gctgtcgagt gctctcgacg cgagatttag ggtgtgggat
    12721 cggttccgct tctcgcggca caactctaat ggtagtccca gatgtatttc aaatagggct
    12781 gcagtttgtt tcggttgcat accttatcga cttttggcgg ccaatacatt tctcggcaaa
    12841 attacgctgc ttttcgggcc caacggggcg cctacaccgc tgggtgaaga tcctcagtgc
    12901 gagccggttg acgacagtag cccgcgtttt ttcggtcgcg aggcatcggt aattcaacat
    12961 gcagtgtggg gagttgcagt gtcgtcgaat ccaatccccg aggctgagca ggtgaaaacg
    13021 aatgactgca acatgacgtt gaagcaatca cgttgcgaaa accgagtcgc cggtcgacca
    13081 cgtaagacaa cagattgcgg gaaatatggt gctgcttcgg gtcaacatgc ggcccggctt
    13141 cgatgtcgac cgctcgctac gattgccgca agcgggaagc gagcgtcagt ccgcagcttg
    13201 gacctcacta acatttcgac ggctcgttcg aggcgaaaag aacgctatcg gccggctgtg
    13261 gggctattga acgtccgagc tctcgctcga ggtttgcaac ttcgccacat gctgattcga
    13321 ggagtcagca agcttcaaat aacccggccg gtttaggttt gctccgcgtc gtcccggtga
    13381 ttcgacaggg tgagcccacc ggcccgagac cagtgccggg ttgcacttgg ggacagtctg
    13441 agtgggcagc cgccgctgtg tcgacgcggt tgcttgcaca ttggttgcag catctcaatt
    13501 ccgttccgcg ctgggaacgt cgtgcaccga gcgcatgcaa cgcgctgccc ctgaccggct
    13561 cagtagtatc gatagcggcg gctgactccg ccggacagca agtgaatcaa cgccacgaac
    13621 acggctgcgc caatgatgga ccagaggatt gggaacgccc gaccttcgat cgcgatggtc
    13681 agcaattcag gcaaatgcat gtagttggca atcacgctac cgagtagggc acccacgaag
    13741 ccgaccgcga tcgaaacgaa gaatcctccg ctcgtgccgc ctgcgatggc tcggccaatg
    13801 gcgccgcaaa taccagcgat gaccagtaag atgagcaagc ctgtgattgt gagcgtcatg
    13861 ggtaagggtg gttgcaaagg ccgcgcctgc ccacagtggc ccagagatct gggtgcagtg
    13921 gaaataggct gcgggttcgc cacagcgtgg cgtgttgacg aacctcactc cgcgccaccg
    13981 gttgggcttc ggttgtgctc ttgattcgtg cagatcagcg aacaatgggg cgagcgcgag
    14041 gctgcgaggg ggcgaaaagg ccaatcgagt tccccgcccg tgcgtgagca ctagagtcac
    14101 tcatacccag tcgtgacctg cgcgttggcg tacgcacgag acgcgaaggc agccctgtgg
    14161 tgtggtcccg ggcagcatgg tggatcgctg ctgcattggc cccgcggggc gcgggcggcg
    14221 caggggacgc tcgatgcagg accgtaaact catgcgggta tttccaccct gcacgtcgac
    14281 gatcaggtac acacccggta acgatcgcga aacgcttcag atcaccgttc cgtcgggcac
    14341 gacggcgccc ttgggaacga tgacgatgcc ttcgcgtatg aagtggttcc cgccatcgtg
    14401 ttgcgtgagg gaacgttcgt tgcggatcat cacccgcctt ccgactcgag cgttcttgtc
    14461 tatgattgca ttctcgatcg tgctgccctc gccgatcccc aatggaatga cggagccgct
    14521 tcgcgcgatc ccttcggagt gggtttcgta gtcgtccgct cccagcagca agctattgcg
    14581 aattttcacg cctctcccga cccgggtgcg cacgccgatg accgaacgtt cgatctgcgc
    14641 ctcctgcagg atgcagccat ccgcgatcaa ggagtctgtc acctggcagc catgcattcg
    14701 agttggagcc aaaaacgcac gctcactgta gatggggtac tctgggtgat agaagtcgaa
    14761 ggtcggggtt tggctcgtga gggccaggtt ggcctcgtag tacgaccgga tcgtgccgac
    14821 gtcctcccag tacccgtcat aaaagaaagc ctgcactcgg ctgcggtcga gcatcatcgg
    14881 caggatgtgc ttgccgaaat cgatgtgact cggatcggct agcgcttttt cgagctccgc
    14941 tctctcgaat acgtagatgc ccatggacgc gaggtaggcc ggccccgtga ccccgggcac
    15001 cgatgaacca aggtcaccca agcgagacgc atgcggcttc tcctcgaagt gagtgactcg
    15061 accgctccca tccaccttca taatgccgaa tccactggtc tggtcggcgg gtactggctt
    15121 catcgccacc gtcacggcgg cccggttttc ccggtggacc cgtaacatat ctcgcaagtt
    15181 catgcggtaa agctggtctc cgctcagaat ggcaatcgcg tcccaactct cccggccgaa
    15241 gtgcttcttg cacttgcgaa tggcgtccgc ggttccccgg aaccaatcat tgccatcatc
    15301 ggtctgttcg gccgcgagga tgtcgatgaa ccctgactga aatgcatcga acttgtaggt
    15361 gtgcgcaata tgccggttca ggctctcgga ctgatattga gtaaccacga agatgcggcg
    15421 cagatcagcg tgaatgcagt tgctcaccgc aacatccacc aagcgatagc ttgccgcgat
    15481 cggcaccgcg ggtttggacc gaaactgagt gagcggaaac aatcgagttc cgcgcccgcc
    15541 gcccagcacc accgcaatga cgttttccat ggtctttctc gtgagatttc ggttggaatg
    15601 aactcttcgg cagaatgttc agcagagtgt tcgtcagaat gttcagcaga gtgtcgaagt
    15661 tcgtgggggg atgtgtcgct cagagcgagt tcgtcatcca cggcttctca gcgacagcag
    15721 gccgacgacc aagctgctac gcgatccttc agggctcacc tcccgcatgg caaattcggg
    15781 gccggcgggc gcggcgtcag cgagagggga ccctgacctc tatcgaatgg ctgaactctc
    15841 gtcatccagt gacgcgccgt acgccccatt ccccgagcaa agccgcagca aggacgcctc
    15901 acatgcgtgc tccgcctcgt gctgggtcgc gcggatggcc gggttaaccg ctcgattgcg
    15961 gtgagcacgg cctttgcctg tacggctgag cagtagcgct gtgtgacttg gatgccgcgg
    16021 tggatgcggc agtcccgatg tccctaccgg caaaggagca gacgcatgac ccagagacac
    16081 acgaccgagg agcaccaccc gaccaacccc aagaaacccg ggccgggtga cactggcaat
    16141 ctaaagaagg atccagacga ctgggttacg ggagacgagc cgatgactca agctcaggct
    16201 tcctacttga agacgctctc cgaagaagcg gacgttccct tcgaagacga gctctccaag
    16261 gcggaagctt cgaagcgcat cgaggagctc cagcaacgca cgggcagggg gcagggctct
    16321 ggctgagagg ccatagagag gggcaccgaa cgcagaggct tcgaccaagc tcgattcgag
    16381 ctaggactct gtgccagctt gtgccagctc gaattcgacc cggacccgcg ttttcctcgc
    16441 aaattcgagg gtcggttggg ggtgcgattc ttgctgctcg gcgcagtagg aggcgcgctc
    16501 gtctgtgaag accgcaaata gcagcagcga acccgcagat agctgcgaac ctgaaggtcg
    16561 aaggccatcg ctgttcagcg ccgcgataga acttacgggg gcctgcaatc agaagtgcgc
    16621 ctattgctat aacgagtggc gcggtgactc gggggccagc atctcgaccg gtggacggga
    16681 taaggtgttg gcgcgcatcc ggcgtctact cgacacgttc gatgttcggg agctgaccct
    16741 gaccgggggg gaaccattta cgcgtccgga tcttttcgaa atcttggacc tgattcgatc
    16801 gcggggcgtc gcagctcaca tcatctcgaa tggcggcttg attgacgaag aacgggcacg
    16861 ggctttggcg gaacgcgagg tgcgcttcgt ccaagtcacg ctgaacggtg ctgaacgaga
    16921 attgcacgag gagcacgtcg ggctcgggca cttcgatgcc accctcgagg ggattcgggc
    16981 cctgcgggcc gcggatgtgg aagtcgtggg ttgcatcgtg gtgacgcgca agaacgccaa
    17041 ccacgtcgcc gatatcctgg agttgttcga ctcccttcgc gtgaggcata ttgctctgag
    17101 ccgcttcagc cccgcgggtt atgcgactcg gcacgtagcc gacctgctac cgtcacgagc
    17161 agatctgatc gctgcgctct ctcgagccgc tcctttcgtg aaggcgcggg gaatgagcat
    17221 tcgagttacg atgccaattc caccttgcgc ggtggagatt gaacagttcc cggacctgat
    17281 attcggagtg tgtccggtgg gcacgccgca acaagagttc gcgattgggc ccgacggagc
    17341 gctgcgccat tgcacgttac atcgcgagaa gttgggcggc ggtctagata tcgctgatcc
    17401 gagcttggac ttggtgaagc tggtgcgggg ctcggaggtc agccagtacc gcgctaccgt
    17461 gccggagttt tgccggggtt gcctacacga gaagacctgc gcggggggtt gcggtgcggc
    17521 ggcagagtgg gtgctcggct cgcgcaatca cccggatccg atcgttgcgc agcacgtaga
    17581 cgaggctttt ggttccgaac tagagcggtt gcgcgaaccg caacgcgttc gattgaacgt
    17641 tctgcacgcg agcgacgatg gctgccgcca cggagcagca gactattcag gagcatgaga
    17701 tggacgacga ttcgaagaag atcatcctcg cccggcgggc gcgattcatc gcggccgcgc
    17761 tggcgagtgt gggcctcgcg gggcaggcct gcggcggcaa gtccgatggg gacggtcaag
    17821 ccatggcagg agggggtggg gcttcgacgg gtggaacccg tagctccacc gggggcgcgc
    17881 cgcagccttg tttatccatc gctacgggag gttctagctt cggaggcgcc atcaataccg
    17941 gggggactag cagcgcaggg ggcgtcgcgt ctgctggtgc cgtgggcacg ggcacgagct
    18001 tcggcggttc gagcggcggc gccaccccgc agccgtgttt gcagccaccc atcgagctac
    18061 cgccagaagg ttccgggggt cgcacgggcg cagccggatc gacgggcacg gccgggtcag
    18121 ctggttcggc aggtcaggca ggcggggctg aggacggggg cacacagaac gagggtggcg
    18181 gagcaaacga aggtggtctg ttcggttcgg ggggagttcc tcaaccctgt ctcgctcctc
    18241 ctcgttagcc accgggcgaa cgaactgcgg actcatgaag cgctcgatcg atcggcttcg
    18301 agttttgctc atctggccgg gggggacgct cggtgccctc tcgaatttcg gcgttccgca
    18361 actgctcagc ctggcgagtg cgatccgagc tcgctgtgat gtcgagcttt cgatcgcaga
    18421 tctggattgc gagcgagcgt tggggccgct cgatctctct cgactctgcc agggtcacga
    18481 tctaatcggg atctcctgct acagctcgtt cgactacctg aaagtgatgg cgttggccgc
    18541 ccgaatccgc gaactttcgc cgcgagccat cctcgtcacg ggcggctacc acccgagcgc
    18601 gtgccccgat gatttcacgc agcccggctc acccttcgac tacgtcgcgg tcggggacgg
    18661 ggaggcaccc ctggcgcgct tgatcgagca gctgagtcgc ggacgacgac cgctcaaccg
    18721 cattctcccg agcgaaccag ttccgagccc gagtgaactt ctcccttacg atttcgagct
    18781 gctcgctcgt tatctgccga tagctcggcg agtggccacg caagccgaaa tctacttgtc
    18841 ccgcggctgc ccctacgatt gctcgttttg catggagcgc gcgaaacgcg agacctcctg
    18901 gcgcgcggtt tcacccgaag aagcgatcga cacgcttcac cagctcgatc gcttcttgga
    18961 cctgcaaggc tggacgctct tcatcgccga cgcgctgttc ggcatgaagc gcgcttggcg
    19021 ccgagagttc ctcgaagcgt tggcgcgcaa accgctgcgc gcgctacggg tgtggttgct
    19081 gattcgagtc gacttgattg agcgggaaga tctcgagttg atgagccgcg ctcacgtcag
    19141 cccgggcttt ggattggagt ccggtgatcc gaatcaactg aagcgaatcc
//
LOCUS       DASTKU010001095        11775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_217662, whole genome shotgun sequence.
ACCESSION   DASTKU010001095 DASTKU010000000
VERSION     DASTKU010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 11775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11775
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217662"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1735
                     /locus_tag="VFQ61_39120"
     CDS             <1..1735
                     /locus_tag="VFQ61_39120"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET9960567.1"
                     /translation="GDASVNHSTALGALNAAGWVSHQNLKLPLLFVCEDNGLGISVRT
                     PPGWITERVRALPHIRPFLARGWDLNETWRVAQEAVEYCRTLRQPALLHIPCVRLLGH
                     AGSDVDTSYRSQQEIDEACDRDPVLCHALALLATGRVAREQLLAQHDAAQARVRAAQE
                     LARSSPTLSSRTQVMAALTGVTRSRVAASLVSTPAPKAGANAESSRRELLSPEEKLTL
                     AQGLNRGLADLLQQHPQALLFGEDVAKKGGVYGVTKGLFERFGALRVFNTLLDEQTIL
                     GLALGSSLAGLLPVAEIQYLAYLHNAEDQLRGEASTLRFFSNAEYDNPLILRVAGLAY
                     QKGFGGHFHNDNSLAVLRDLPGVVVAIPARSDDALALLRSCRQLALEQGRVCVLVEPI
                     ALYHRRGLLQEEDGLWLAADDGRTAEFGRARVYHETAPDLTILTYGNGLALSLAAAQR
                     LRAQNIGARVLDLRWLAPLPEQDILEHAAKTGRVLVVDECRHSGNVSEQIAATLLDAG
                     YQGAFRRVTGADSFIPLGEAARHVLVSEDEIVEAARSLAALTAPDPLASWAKQLPPQI
                     GKLWRSLRKAL"
     gene            2052..4253
                     /locus_tag="VFQ61_39125"
     CDS             2052..4253
                     /locus_tag="VFQ61_39125"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9960568.1"
                     /translation="MSTPASYPEGETTTEQVELELVVNAEGRVTEVRAISGPGRFRER
                     AEAASQRWHFDPATREGVPVTARVRFVLTFEPPRPSSSAETNEETPPASSAHQTTSPN
                     LEAQRGEHGKGAREESPEQEILVVGERQTLRTRLSRVDVRELPGAFGDAYRAIEVLPG
                     VVPIASGVPYFYVRGAPPGNIGYFFDDIPVPLLYHFAAGPAVLHPAFVGNVDLYPGAY
                     PARYGRFAGGIVAGEIAAPSYRVRGEASIRIVDSGAMLELPFADGRGSAMLAGRFSYT
                     AALLSLISPDVNVGYWDYQGRVRYALTSRDSVEVLGFGSRDVVTEQERAFGRETTRTL
                     VNLGFHRVALRWERALPRGRLRTGLLLGLDKTYAGDEGEIELTSRMIGARAEYERAVS
                     ESTSWRAGVDVLTQSYSQQLAIDVSEGSASAPSPADSSDAARFGFAQEHTDYVATLFA
                     ELTLNPTRNIQVVAGLRGDTFASEGQWRLGIDPRISARFTLGHGWTMKHGLGVAHQPP
                     SFIAAIPGASPSLTGKLQRTIQHSVGVERALPAGIRASVTLFQNLFFDMTDYIGLVQQ
                     QTTVDREDNLRMGGRGFGAELLLQRSLAHSIGGFLSYTVLRSERYSGEFSGPTTTDRT
                     HVLNLALSYDLGKNWRFGNRFMFYSGVPARVAYLEAARHPPRAPGFYRLDIRLQKRFW
                     YGPERYWGLVLEVLNTTLHRETLDRSCNAFDCRDNPFGPVTIPSVGVEGAF"
     gene            4765..5406
                     /locus_tag="VFQ61_39130"
     CDS             4765..5406
                     /locus_tag="VFQ61_39130"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET9960569.1"
                     /translation="MTPLYPIIDLDTLSSRGTDPLRFAQSVLTARPAMLQLRAKTQST
                     REALEWLDRLAPLCRAHGTELYANDRPDLAVLAGASGVHVGQTDLPVSAVRRFSRQLK
                     IGVSTHNLAQLKAALADRPDYVALGPIFATSSKQNPDPVVGWSTLEEASQLCQVAGIE
                     LVAIGGIEERHLPRMAELSVSVAVISALCAPSYEEVEQRARRFGALLGGAAGD"
     gene            complement(5436..6401)
                     /locus_tag="VFQ61_39135"
     CDS             complement(5436..6401)
                     /locus_tag="VFQ61_39135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HET9960570.1"
                     /translation="MTFDGIWGQDPALQTLKRALTSGHVHHAYRFEGPDGVGKELTAF
                     RLAQALVCGESPLGCGVCSACERAISLSAEAPHLPRHPDVVLLGRGLYASVTGQSEAG
                     GIGVEQIRKVIQSRVGYGPHEGRALVFIVRDAEELTLQAANSLLKTLEEPPPATHFVL
                     LSSRPNRLLDTIRSRTLPVRFAPLPDATVAQLLRERGLDEQVASLAQGSMTRALLLCD
                     PDSLAERQAFLSGIAAALAAPDLSATTRFAESNKGSRDVIKQSLGYYAQELSIQARRE
                     LEAEPREAERAARRHGVVLEAMNEIERNVQPALVLESMLYRLRRV"
     gene            6454..7548
                     /locus_tag="VFQ61_39140"
     CDS             6454..7548
                     /locus_tag="VFQ61_39140"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9960571.1"
                     /translation="MLAARSSFRDSWISVGLWVGLASAGLSGAGCAAPANNVTRIARG
                     IEHKGREISDLAYALYARGAVWEAQGHLDEALTAYTKALDEDPGAPELLARIGAVRCK
                     RSQSGDGRSLSRALEDFERALRWDPENSLAYEELSRCHTRHAQYARALRAALEAVSHD
                     PQAEHLSLLVAEAAQAAGDGSTADRWLDATVTWHPNSIAAWLAFERHASRRRDPIRLL
                     RSRRALERLGGARLDPDRFQAKRDPRRLPPADAARSHSPAAESSPETGAAKRAFEANP
                     DDAEAWVKLLVSADLAGQSEDFERYLRAAPQDPGSLSANAAQDYAVLLERWVDTSARR
                     AWEAQQLQTAGSSVSTTAPTAAPQSGQARP"
     gene            7963..8307
                     /locus_tag="VFQ61_39145"
     CDS             7963..8307
                     /locus_tag="VFQ61_39145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960572.1"
                     /translation="MRKVWLVWAAAVLLGASGCFLIQGRAELVKRDKAGGVLALKGDR
                     NKAMEDAKRQMSANCPEGYEIVGEEMAKVGETTEAAEDAAFGKKSASKSSTSVTTDVK
                     EYRITYSCNRPE"
     gene            complement(8476..9438)
                     /locus_tag="VFQ61_39150"
     CDS             complement(8476..9438)
                     /locus_tag="VFQ61_39150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960573.1"
                     /translation="MPGRHLLLALLALLQAAALGACKKSSPSEPAGGPAASASSFVLP
                     APVASGIPTPTRDISSVVNPKGEPPYTGPAGKVTGRVRVTGDSAPELPEAVRAISDEC
                     APAREVYGKAFREGVDRALADVLVGVTGYSGYVPPASSVVKVPAAGCAWSARTFALTF
                     GQRIDVEAKDRKAYIPDLLGARMPAQLVALPGGSGSSLYPTSPGRYVLVDSLRIFSRA
                     EVLVLKYATHDVTGLDGRFTISGIPAGKVTLSAYFPATGQMLDREVVIPAGGTLEQDL
                     EFAFNAKAFAEAQERARSESASSAKSSASAASPPASGSSAPARK"
     gene            complement(9438..10985)
                     /locus_tag="VFQ61_39155"
     CDS             complement(9438..10985)
                     /locus_tag="VFQ61_39155"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET9960574.1"
                     /translation="MNVLLSLSPILVVSLGALLLMLAEAFGKPLSAEGIGPDGVVIDA
                     GAGRSGELALGAAVALFAGARASVGLWLVGPEKLGGAESIAPYLVMDRFALFFCFTLC
                     LGGGLTVLLAGGYLTEHNLDRGEFYPLVLFATIGAMALAAAGDLLSLFIALETMSLGA
                     YCMIGLRRGNVRASEAAVKYFLLGSFAAALMLFGAAIAYGATGHTDLSGIGKAVATIG
                     QAGSTVSAGGILIALVLILAGLAFKISAVPFHMWTPDAYEGAPTPATTFMAVAVKSAA
                     FAMLLRVLLVAFGDARLLSWGTGWPPALSVLAVVTMTVANLIAGRQESVKRMLAYSSI
                     AHAGYALVGVVAAMKSGAAVPSVLFYMLGYTVSTVGAFGALILCGRRGAEAVSYEDLA
                     GIGRRHPAAALAVSFFLLSLAGVPPTAGFFGKLYVFAAAVDAELYPLAIIGLLNSVVG
                     AYYYLRVLVFMYMREPAPGAPIATPMRSGLVSAALVIAALFVLGIGMLPNFSLELARA
                     AGLQAGG"
     gene            complement(10982..>11775)
                     /locus_tag="VFQ61_39160"
     CDS             complement(10982..>11775)
                     /locus_tag="VFQ61_39160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET9960575.1"
                     /translation="IGGGIIYGALVAWKQDDVKRLVAYSSVAHMGFVMLGLFGGTESG
                     IAGSVLQMINHGVSTGALFLLVGVIYDRRHTRLAREFGGLAKIMPVYTVLFLIVTMSS
                     IGVPGTNGFVGEFMVVLGTFVSQALGPQAKLQGTLAAAGVILAAVYMLSVVQKMFFGP
                     LDNPKNKRLKDINPREVVAVAPFIALIFVIGFFPNLFLSRMTDSVRDVVERYENGFRS
                     YRESDPEAKLATLRPRRGGPLEVGYPEAPQASGGAEQAANTEPAK"
BASE COUNT         2234 a         3836 c         3739 g         1966 t
ORIGIN      
        1 cggtgacgcg agcgtcaatc acagtacggc actcggtgcg ctcaacgccg cgggttgggt
       61 aagccatcaa aacctgaagc tgcctctctt gttcgtatgc gaagacaacg gactcgggat
      121 cagcgtccgt acaccgcccg gctggatcac ggaacgcgtg cgcgccttac cgcacattcg
      181 cccattcctg gcgcgtgggt gggatctgaa cgagacatgg cgtgtggccc aggaagccgt
      241 tgaatactgc cgcacgctac gtcagcccgc cttgctgcat atcccctgcg tccgcctgct
      301 cggacacgcg ggcagcgacg tcgacaccag ctatcgctcg cagcaggaga tcgatgaagc
      361 gtgcgaccgc gatcccgtgc tctgccatgc gttggccttg ctcgcgaccg gccgagtcgc
      421 tcgagagcag ctcctcgcgc aacatgatgc ggcccaggcg cgcgttcgcg ccgcccaaga
      481 gctcgcgcgc tcgagcccaa ccctctccag ccgaactcaa gtcatggccg cactaaccgg
      541 agtgacgcgc agccgggtcg cggcctcact cgtgagcacc cccgcaccga aagccggagc
      601 gaacgcagag tcgtcccggc gtgagctgct gagcccggaa gaaaaactga cgttggcgca
      661 gggtttgaac cgggggctgg cggacttgtt gcagcagcat cctcaggcgc tgctcttcgg
      721 ggaagacgtc gcaaaaaaag gtggagtcta cggcgtcacc aaaggcctgt tcgagcgttt
      781 cggggcgctg cgtgtgttca acacgttgct cgacgagcaa accatcttgg ggttggcgtt
      841 gggcagtagc ttggcgggcc tgctgcccgt ggcagagatt cagtatctcg cctacctgca
      901 caacgcggaa gaccagctcc gcggcgaggc atccacgctg cgattctttt cgaatgcgga
      961 gtacgacaac cccttgatcc tgcgcgtggc tgggcttgcc tatcaaaagg gttttggcgg
     1021 ccactttcac aacgacaact cgctcgccgt actgagagac ttgccgggcg tcgtggtcgc
     1081 aattcccgcc cgctctgacg atgcgctcgc gttacttcgc agctgccgcc agttggcgct
     1141 cgaacaaggc cgagtttgcg tgttggtcga accaattgcg ctgtatcacc gacgtggctt
     1201 gctccaggag gaggacggcc tttggttggc cgcagacgac gggcgaacgg ccgagttcgg
     1261 tcgggctcgc gtctaccatg agaccgcgcc ggatctcacg atcctgactt acggaaatgg
     1321 gctcgccttg tcgctcgcag cagcgcagcg tctgcgagcg cagaatattg gcgcgcgcgt
     1381 tttggacttg cgctggctcg cccctctgcc ggagcaggac atcctcgaac atgctgcgaa
     1441 gaccggccgt gtgctggttg tggacgaatg ccgtcattcc ggaaatgtct ccgaacagat
     1501 cgcagcgaca ttgctcgatg ccgggtatca gggcgcgttc aggcgggtca ccggcgcgga
     1561 tagcttcatc ccgcttggtg aggcggcgcg ccacgtattg gtgagcgagg atgagatcgt
     1621 agaggccgcg cgctctcttg cagctctgac cgcaccggac ccgctcgcga gttgggccaa
     1681 acagctcccg ccccagatcg gcaaactctg gcgttcactc cgcaaagcac tgtgagaagg
     1741 ccgctgaacg caagcgacgg aattaatgct gcacctcgtc cgcttcgtgc ggaccaagag
     1801 cccaagcttg gtgtacgctc gcgccgctag tgggtcgagt ttggtttccc ctcacctgtg
     1861 cgctcctgct cggcaagctg gcgggcgcgc gagcgcaaac gcaaccagcg caaacgcaaa
     1921 cgcaaccagc gcaaacgcaa gcgcaaccag cgcaagcgca acccgggagc gctgagccta
     1981 agcccggagc cgcggattcg aatgtgactc cgccggccgc aaagtccgct cgggtgaccg
     2041 cgccacgcgc cgtgtcgacg ccagcctctt atcccgaagg agaaacgacg accgagcagg
     2101 tcgaactcga gctggtcgtg aacgccgaag ggcgtgtcac cgaggtgcgc gccatctcgg
     2161 gcccagggcg cttccgcgaa cgagcggagg cggcgagcca acgctggcac ttcgaccctg
     2221 cgacccgcga aggtgtaccc gtcacggctc gcgtgcgctt cgtcttgacg ttcgagccgc
     2281 cacggccttc gtcgagcgca gagaccaacg aagagacgcc gccggcgagc tctgcgcatc
     2341 agactacgtc tcccaaccta gaggcccaac gaggggagca cggaaaaggc gcgcgcgagg
     2401 aaagccccga acaagagatc ctagtggttg gggaacgaca gaccttgcga acacgactga
     2461 gtcgcgtcga cgtccgagag ctcccgggcg ccttcggcga tgcttaccgc gccatcgagg
     2521 tgctgccggg agtcgtaccc attgcttccg gcgtccctta cttttacgtg cgcggcgcgc
     2581 cacccggcaa tatcggttac ttcttcgacg atatccccgt cccacttctt tatcacttcg
     2641 ccgcggggcc cgcggtgctg catcctgcgt tcgtcggcaa cgtcgacctg tatccgggcg
     2701 cctaccccgc tcgctacggt cgcttcgccg gcggcatcgt cgccggtgag atcgccgcgc
     2761 cgagctatcg cgtgcgtggt gaagccagca ttcgaatcgt ggacagcggc gcgatgctcg
     2821 agctgccctt cgccgatggc cgcggaagcg cgatgttggc cgggcgattc tcgtacacgg
     2881 cggcgctgct gtcattaatc tcacccgacg taaacgtggg ctattgggac taccaaggtc
     2941 gtgtccgcta cgctttgact tcacgggact cggtcgaggt gctgggcttt ggttcgcgcg
     3001 acgtggtgac ggagcaggag cgggccttcg gtcgggagac cactcgcacg ttggtcaatc
     3061 tcgggtttca ccgcgtagcc ctgcgttggg agcgcgccct gccgagggga aggttgcgca
     3121 cgggcctgct gctcgggctc gataaaacct acgcgggcga cgaaggcgaa atagagttga
     3181 ccagtcgcat gatcggcgcg cgcgcggagt acgagcgcgc cgtttccgag agcacttcat
     3241 ggcgagccgg agtcgacgtc ctgacgcaga gttactcgca gcagctcgcg atcgacgtct
     3301 ccgaaggttc ggcgtcagcg ccatcgccag cagattcctc cgacgcagcc cgcttcggtt
     3361 tcgcgcagga gcacacggac tacgtcgcga cgctcttcgc cgaactgacc ctgaacccga
     3421 cgcgcaacat tcaagtcgtc gcggggctac gcggcgacac cttcgcgtcc gagggacagt
     3481 ggcgtttggg aatcgatccg cgcatcagcg cccgcttcac actcgggcac ggctggacga
     3541 tgaaacacgg cctcggggtc gcgcatcagc cacccagttt catcgccgcg atccccggag
     3601 ccagccccag cttgaccggc aagttgcagc gcacgatcca acacagcgtc ggagtcgagc
     3661 gcgccttgcc agccggtatt cgagcgtccg ttacgctatt tcaaaacttg ttcttcgaca
     3721 tgaccgatta catcggtctc gtacaacagc aaacgaccgt cgatcgtgaa gacaacctgc
     3781 gcatgggtgg acgaggtttc ggggcggagc tgctgctgca acgcagcctc gcccactcga
     3841 tcggaggatt cctgtcgtat accgtgttgc ggtcggagcg ctattccggt gagttctcgg
     3901 ggcccacgac gactgatcgc acccacgtac tcaatctcgc gctgtcctac gatctcggga
     3961 agaattggcg gttcggaaac cgcttcatgt tctattcggg cgtcccggca cgagtcgcct
     4021 atctagaagc ggcgcggcac ccgcctcgcg cgccgggctt ttaccgcctg gatattcggc
     4081 tacagaagcg tttctggtat ggccccgaac gctattgggg gctggttttg gaagtgctca
     4141 acaccactct gcatcgagag acgctcgatc gcagctgcaa tgcatttgac tgccgcgaca
     4201 atccatttgg cccggttact atcccgagcg tgggggtaga aggcgcgttt tgaaccggag
     4261 taggcgattg ctggtcaacg ccaagcggcg aaagaatggg ggcacctgag cagccccgac
     4321 atggctcgta gacacggttc gcccatcagc tcacggccgt cgaacgctcg cgccgacttc
     4381 acgctggcac agaccaccgc gtccggagca tctcggaagc aggcgatgac cgtgtcccag
     4441 tgtgcgcgac aggttgggtc agcgggcggg ttgccttccg cttcgcaggc gctgacgcac
     4501 ccggcggggt tgtagaattc gtcgcaagaa acccgaaagg ttgcgtggca catctcatca
     4561 caaggcgtcg gcggcagcgg gtagtcgttg ccacacgagg ataaccacag ccccccgctc
     4621 aataggacgg tccacccgcg gctccgagac ctcgagcgcg agacgccccc cgaatcacgc
     4681 tcgattttgc acttcgcccg gcgcactctg cgagcttccg ccaacccggc agcgagatca
     4741 atcccgtcga gctatggttc gcgcgtgacg ccgctctatc ccatcatcga tctcgatacg
     4801 ctatcgagcc gaggcacgga tccactgcgc ttcgcgcaaa gtgtgctcac cgctcggccc
     4861 gccatgctgc aactccgcgc caagactcaa agcacgcgcg aagcgctcga gtggctcgat
     4921 cgattggccc ccctgtgccg agcccatggc accgagctct atgccaacga ccgccccgac
     4981 ttggccgtcc tcgcgggcgc cagcggcgtc cacgtgggtc aaacggattt gccggtaagt
     5041 gcagttcggc gcttctcccg gcagttgaag atcggcgtat cgacccacaa cttggctcag
     5101 ctgaaggcgg ccttggcgga tcgcccggat tacgttgcgc tgggccccat tttcgcaact
     5161 agttccaaac aaaacccgga tccggtcgtg ggctggtcga cgctggagga ggcgagccag
     5221 ctttgccagg tcgcgggtat agagctggta gcaatcggcg ggatcgaaga acgacatcta
     5281 ccccgcatgg ccgagctctc ggtcagcgtc gccgtgatat ccgcattgtg cgcgccgtcc
     5341 tatgaagagg tcgagcagcg cgctcgccgc ttcggtgcgc tgttgggcgg tgccgccgga
     5401 gattagtgga gtcaggcgcg ccgcggtcgc acgcttcaga cgcgtcgcag tcggtacaac
     5461 atcgattcga ggaccaaggc gggttgcacg ttgcgctcga tttcattcat ggcctcgagc
     5521 acgaccccat ggcgacgcgc ggcgcgttct gcttcccgcg gttcggcctc gagctcccga
     5581 cgcgcctgga tcgagagctc ctgcgcgtag tagcccaggc tctgtttgat cacgtctcga
     5641 ctacctttgt tgctctcagc aaagcgcgta gttgccgata aatcgggggc cgcgagcgcg
     5701 gccgcgatgc cggagagaaa ggcttggcgc tcggcgagtg agtcgggatc acacagcaac
     5761 agcgcccgcg tcatactgcc ctgagccaac gacgccacct gctcgtcgag ccctcgctca
     5821 cgcagcaact gagctacggt tgcgtcaggc aagggcgcga agcgtacggg gagcgtacgc
     5881 gaccgaatcg tgtccagcag ccgattggga cggctggaaa gcaagacgaa gtgtgtagcc
     5941 gggggcggtt cctcgagggt cttcaagagc gagttcgccg cctgcaacgt cagctcctcg
     6001 gcgtcacgca cgatgaacac caatgcccgg ccttcatgtg gtccgtaccc gacacgcgac
     6061 tggatcactt tacgaatctg ttcgaccccg atgccacccg cctcgctctg cccagtgacg
     6121 ctcgcgtaaa gcccccgccc gaggagcacg acgtcgggat gccgaggcaa gtgaggcgcc
     6181 tcggcgctca aggagatcgc ccgctcgcag gctgaacaca cgccgcagcc caggggcgat
     6241 tcgccgcaca ctagcgcttg tgccagacga aacgccgtaa gctctttgcc gacgccgtcc
     6301 ggaccttcga agcggtaggc atggtggacg tgcccgctcg tgagagcgcg cttcaaggtt
     6361 tgaagagcag ggtcttggcc ccaaattccg tcgaaggtca tggcagtccg cccacgcggg
     6421 tggtggagtc gtgatagtac tccgtcccgc ttcatgctcg ctgcccgttc ttctttccgg
     6481 gattcgtgga tcagcgtcgg gctgtgggtg gggttagcga gcgcgggcct gtccggcgca
     6541 ggctgtgcgg ctccggccaa caacgtcacc cgtatcgcaa ggggtatcga gcacaaggga
     6601 cgcgagattt cggacttggc ctacgcgctc tacgcgcggg gcgcggtgtg ggaagcgcaa
     6661 gggcatctcg atgaggcgct caccgcgtac acgaaggccc tcgacgagga ccccggggcg
     6721 cccgagctct tggcccggat cggggcggtg cgctgcaagc gcagccaatc aggcgatggc
     6781 cgctcgctct cgcgcgcgct cgaggacttc gaacgagcgc tacgctggga ccccgagaat
     6841 tcactggctt acgaggagct gtcgcgctgc cacacgcgcc acgcgcagta cgctcgggct
     6901 ttgcgggcgg cgctcgaagc ggtgtctcat gatccacagg ctgagcactt gagcctgctg
     6961 gtcgccgagg cggcccaggc cgcgggcgat ggcagcaccg cagatcgctg gctagacgcg
     7021 acggtgacat ggcatccaaa ctcgatcgcg gcttggctgg cgttcgagcg ccatgcgtcg
     7081 cgtcgacggg acccaatccg cctgctgaga tctcggcgag cactcgagcg gctaggtggc
     7141 gcgcgtttgg atccggatcg cttccaagca aagcgcgacc caaggcgcct gcccccagcc
     7201 gacgcagcgc gctcccattc tcccgcagca gaaagttctc ccgagaccgg ggccgccaaa
     7261 cgggcgttcg aggctaaccc cgatgacgcc gaggcctggg tgaagctgtt ggtttcagcg
     7321 gatctggccg ggcaatccga agatttcgag cgctacttgc gcgcggcgcc ccaagatccc
     7381 ggttcgctct ccgcgaatgc ggcacaggac tacgccgtgc tgctcgaacg gtgggtcgac
     7441 acgagtgcgc gccgtgcctg ggaagcacaa caactccaaa cggctggaag ctccgtctcg
     7501 accaccgcgc caaccgcggc cccgcagtca ggtcaagccc gaccttagcc ctccgaatta
     7561 tcgaagcccc cgcggagacg cagcgctcag ttttgaaacc gggccaggga cggcctttcg
     7621 gcgtgggaga aaaagaagcg gtcaacgaga tcgtgttgaa taaaccgctc tgcgcggcgt
     7681 ctatgtgacc gcgtcccggt agccgtctat ctctcgggtg acgccgagat gtcgacgctc
     7741 cgaatcgcag gttggtcgag tcgggtttgg ttgggtcaac gcagtgaaga acagcccaac
     7801 gggcaaaata ggcgggaact gggcaactag ccgctagctg tcgggctgcc tcgctttcca
     7861 gtcgcgggca tgagcgtgct ggcatacagg cagtcttgac ggcaccagct ggtaaagctg
     7921 gttcagcgct gtcgccccgc ttacctgccg gaggttgagc agatgcggaa ggtttggttg
     7981 gtgtgggcag ccgctgtgct gctgggtgct tcgggttgct tcttgattca aggacgagcg
     8041 gagctcgtga agcgggacaa ggccggcggg gtgctggcgc tgaagggcga tcgcaacaag
     8101 gcaatggaag acgccaaacg ccagatgtct gcgaactgtc ccgaaggtta cgaaatcgtc
     8161 ggagaagaga tggcaaaggt gggcgagacc acagaagccg ctgaagacgc ggctttcggc
     8221 aagaaatccg cctccaagag ctccacctcg gtcaccacgg acgtgaagga gtatcgaata
     8281 acgtactcct gcaaccgacc ggaatagatc gacttcgttc agagtcgcgc gtcatcagcg
     8341 ttcgcgctcg ggagcgccga aagctcgagc ggagggcgtt gatgacgccg caatcactga
     8401 gtcccccgct gcgtttccga ggcggtgccg ggcgcgaaaa gctgcgcgcc cacgctcaga
     8461 ctactcttga agcgtctact ttcgagctgg agcagatgag cccgatgcgg gtggagacgc
     8521 cgcactcgca ctcgatttgg ccgatgacgc gctctcgctg cgcgctcgtt cctgagcttc
     8581 ggcgaaagcc ttggcattga acgcgaattc gagatcttgc tcgagagttc ccccggccgg
     8641 gatcacgact tcgcgatcga gcatttgccc agtcgctggg aagtacgcgc tcagcgtcac
     8701 cttgccggct ggaatgcctg aaatcgtgaa gcgcccatcc agcccggtaa cgtcgtgggt
     8761 ggcgtacttc agcacgagaa cttccgctct cgagaagatg cgcaagctat cgaccagcac
     8821 gtagcgcccc ggagacgtcg gataaaggct gctcccactg ccaccaggca gcgcaaccag
     8881 ctgtgcgggc atgcgcgcgc cgagcagatc ggggatatag gccttacgat ccttggcctc
     8941 gacgtcgatg cgctgaccga acgtcagtgc gaacgtgcgc gcggaccagg cgcagcccgc
     9001 agccgggacc ttcaccaccg aagaggccgg aggcacataa ccggagtacc cggtcacgcc
     9061 caccaacaca tccgcgagcg cacggtccac cccctcacgg aaggccttgc cgtagacctc
     9121 gcgcgccgga gcgcattcgt ccgaaatcgc tcggactgcc tcgggcagct cgggagcgga
     9181 gtcccccgtc acgcggactc tgccggtaac tttgccggcg gggccggtgt aaggcggctc
     9241 gcctttggga tttacgaccg acgaaatatc ccgcgtcggt gtggggatcc ccgaagcaac
     9301 cggagctgga agcacgaaac tcgacgcgga agccgcgggg ccccccgctg gctcactcgg
     9361 actcgacttc ttgcaggcgc ccagggcggc cgcctgcaag agagccaaca aggccagcag
     9421 gagatgacga cccggcatca accgcctgcc tgaagaccgg cggcgcgggc cagttccagt
     9481 gagaagttcg gcaacatgcc aatgccgagc acgaacagtg ctgcgatcac gagcgccgcg
     9541 ctcaccaaac ccgaacgcat gggcgtggcg atcggtgcgc ccggcgcggg ttcacgcatg
     9601 tacatgaaca cgagcacgcg caggtagtag taagcaccca ccacgctatt cagcagaccg
     9661 atgattgcca gcggatagag ctcggcgtcc acggctgcgg cgaagacgta cagcttaccg
     9721 aagaaacctg cggtaggcgg aacgcccgcc aaggacaata ggaagaacga taccgccaac
     9781 gccgccgccg gatgacgccg tccgatgccg gccaagtctt cgtagctcac ggcctccgct
     9841 ccacgccggc cgcaaaggat cagcgcgccg aaggcaccca ccgtcgagac ggtgtagccc
     9901 agcatgtaga acagcacgct cggcactgct gcccccgact tcatcgccgc gaccacgccg
     9961 accagcgcgt aacctgcgtg cgcaatcgac gagtaagcca gcatgcgctt gaccgactcc
    10021 tggcgaccgg caatcaagtt cgcgaccgtc atggttacga ccgcgagcac cgaaagcgcc
    10081 ggcggccaac ccgtacccca gctcagcagt cgggcatccc cgaatgcgac gagcaggacc
    10141 cgaagcaaca tcgcgaaggc cgcgctcttg acggccaccg ccatgaacgt ggtggccggc
    10201 gtcggggcgc cttcgtaagc gtcgggcgtc cacatgtgaa atgggacggc gctgatcttg
    10261 aaagccaatc cggccaagat cagcacgagc gcgatcagga tcccgcccgc actgacggtg
    10321 ctgccggcct gaccgatcgt cgccacggct ttgccgatgc cggatagatc cgtgtgaccg
    10381 gtcgcgccgt aagcgatcgc cgcgccaaag agcattagcg cggccgcgaa gctgccgagc
    10441 aagaagtact tcactgccgc ctcgctcgcg cgcacgttgc cgcgtcgcaa gccgatcatg
    10501 cagtaagcac cgagcgacat cgtctcgagc gcgatgaaca agctgagcag gtcgcccgct
    10561 gccgccagcg ccatcgcgcc gatcgtggcg aacagcacga gcggatagaa ctcgccgcga
    10621 tccaagttgt gctcggtgag gtatccgccc gccaacagca cggtgaggcc accgccgagg
    10681 cacagcgtga agcagaagaa cagagcgaag cgatccatca ccagatacgg tgcgatcgat
    10741 tcggcgcccc cgagcttctc gggcccaact agccacagtc cgacgcttgc cctcgcgccg
    10801 gcaaacagcg ccacggccgc acccagcgcc agttcgcccg aacgccccgc accggcgtcg
    10861 atcacgacgc cgtccggacc aatgccttct gcgctcagag gcttgccgaa cgcctcggca
    10921 agcatgagca acagcgcgcc gagggagacg accaaaatcg gagataacga aagcagcacg
    10981 ttcattttgc gggctccgtg ttggcagcct gttcggctcc gccactcgcc tgaggcgcct
    11041 ctgggtaacc gacttcgagc gggccgccgc gtcgcggacg caaggtcgcg agcttcgctt
    11101 ccggatccga ttctcgatag gagcggaagc cgttctcgta acgctcgacg acatcgcgga
    11161 cgctgtcggt catgcgcgac aggaacagat tggggaagaa gccgatcacg aagatcagcg
    11221 cgatgaacgg cgcaaccgcc accacttcac gcggattgat gtccttcagg cgcttgttct
    11281 tcgggttgtc gagcggcccg aagaacatct tttgcacgac gctgagcatg taaaccgccg
    11341 ccaggatcac gccggccgcc gccaacgtgc cttgcagttt cgcctgcggg ccgagcgcct
    11401 gggacacgaa ggtgcccagc acgaccatga actcgccgac gaagccattg gtcccgggca
    11461 cgccgatgct ggacatcgtc acgatcagga acagcacggt gtagaccggc atgatcttgg
    11521 caagaccgcc aaactcacgc gcgaggcgcg tgtggcggcg gtcgtagatg acgcccacca
    11581 acaagaacaa cgcgccggtg gacacgccgt ggttgatcat ctgcagcacg ctgcccgcga
    11641 tgccggactc cgtgccgccg aacaaaccga gcatcacgaa gcccatgtgc gccaccgacg
    11701 agtaagcgac caggcgcttc acgtcgtctt gcttccaggc gacgagcgcg ccgtagatga
    11761 tgccgccgcc aatgg
//
LOCUS       DASTKU010001096         9618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1111266, whole genome shotgun sequence.
ACCESSION   DASTKU010001096 DASTKU010000000
VERSION     DASTKU010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111266"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFQ61_39165"
     CDS             complement(<1..635)
                     /locus_tag="VFQ61_39165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960576.1"
                     /translation="MPETISSLDPLATQLAPPLELAARIRSAVAQTPAPRSSLRERRL
                     WLLVAIPFAVAAGPLGSHLFFRTRPTLRVDLAQVPWASLTERLGALLLLASIATGVVV
                     APGRRGLGAQVLLLLTAAIAVTPLYTLVTTLGPLQTPAGQLASRELHPLGLPCAGVAV
                     AVGALMLIAFTVALRRSVPVASTTRAAALGAAAGAWAGLALFLQCPASDAL"
     gene            complement(628..1200)
                     /locus_tag="VFQ61_39170"
     CDS             complement(628..1200)
                     /locus_tag="VFQ61_39170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET9960577.1"
                     /translation="MNADEAMRRYAGGDAAAFEELYAALASRLYSFCVRLAGRGGDAD
                     DLFQEAFLKLHRSRGTYAPGSNALHWAFAIVRSTYLDRLRARTRRPEELFASVEEGPG
                     ENSGEAHASPESVLHAEALARLVERELQRMSERNRSAYVLLKLEGLTAAEAAAVLGTT
                     GEAVKQRAFRAYEALREALSRAGWDRINRA"
     gene            1601..2911
                     /locus_tag="VFQ61_39175"
     CDS             1601..2911
                     /locus_tag="VFQ61_39175"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HET9960578.1"
                     /translation="MIVSWPSEDLAGESLVHHCLAGGEQLQSVPNSEAPYLASLPAST
                     ASAGEAVDPFLPVVGATMAGIVEMLRVFAQQAEPILISGPTGAGKSRLARWCHEHSAA
                     QKGAFEMLDLSSVPEELQMAELFGWRRGAFTGAVRDNPGSIARARGGTLFIDEVDNLS
                     PRAQAGLLHVLEDRSYRVLGDNGGEKPAEVRFIVGTNARLSEAVREKRFREDLYYRIN
                     VLPVRLPALRERADEIPMWARYSANRHHTMRVPEGRVELSARAELALAREPWPGNIRQ
                     LDNIIRRAYTIALLAHGGATPRDLVLEEDHILRALAYEGSEQRGSLVQAMLEAAAAFV
                     SEAESGTVAGRPLDLDLADSFKGFVLGVASERRGSREEAFRLFGRERLVTSRNHQKVF
                     KRELERTENLCRSIGKADEFPFVRCVASEEEAPKEETHPNGLAQ"
     gene            complement(2990..4303)
                     /locus_tag="VFQ61_39180"
     CDS             complement(2990..4303)
                     /locus_tag="VFQ61_39180"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HET9960579.1"
                     /translation="MVRAAQTGALLGVLASSACSNIFGVGELTSSQEGTRDAGASTAA
                     GSAGGGTSAGRVGAAGANTTAISGVPSTHSGGSSTSANSATGSPSSQGGSSFSSTDGG
                     SGATPDQGGRTQDSFSFSGGVRSTLTPSTGGAGASDVPPSCQELAATCGASSFGSGDC
                     CASKTVPGGRFFRSYDRVPAGDFDSTAWPAEISPFQLDVYEVTVGRFRQFVAAYPWRP
                     AAGAGRNPSNPSDSGWRDEWNTQLLRDAVGLANALESCESPRNLQTDSSSTWTGEPEG
                     QEELPINCVTWFEAFAFCAWDGGRLPTEAEWNYAAAGGDEARVYPWPSTLLTEVDMNH
                     AVYQVPIAPVGSKPMGLGLLGQADLAGNVAEWNLDVYREPYGVPCNDCAELDSGSSNL
                     VRVLRGGSYADQGQRLRSAERSSAPPNTRDPRYGFRCARDSLTNE"
     gene            complement(4297..5346)
                     /locus_tag="VFQ61_39185"
     CDS             complement(4297..5346)
                     /locus_tag="VFQ61_39185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960580.1"
                     /translation="MTLLVPALWLPTAQAQKREQAPTNITAQTALDAAKTPPEPSELP
                     RSSDDSTRAAALDEEGTALLNQRRLSEACPRLAESFRLHPGTGVLLRLALCHELSRNA
                     ATAQSLYSRAAARARTAGDVEVAQLADKRAAELQPRISHLIVRFAPSTARVPNVTLLY
                     DGSPLPTAELNSPLAVDPGPHVIEARAPGHPPFRQEVRVNEAATNYWITVVMAPNRVA
                     SLKPPPVSPAPRPAWPTQKTWALALGGIGIGAVTAGTIFGLTVASRMDRARKGCTDGH
                     RGCSDEALELQDSARTPALASTLAFSAGAVSIAGALALWFMAPPDARETRDASLRAVP
                     LLGPGSAGVQAVGRW"
     gene            complement(5438..6973)
                     /locus_tag="VFQ61_39190"
     CDS             complement(5438..6973)
                     /locus_tag="VFQ61_39190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9960581.1"
                     /translation="MRTIPIGEVVDGRYVIERIAERSACGIVATARHVALNRLVALKF
                     FWPTTPKDALFLQRVLTDVRAAAQLRSEHAARVLDAGTLGDGSVFVIYEHLPGRDLSA
                     VLRKDGPLPLTVAVDYITQACEPLAEAQALGIGRRALQPSDLFLTRGLDGTPSIKVLD
                     FGLGQCDKRPNVSRGDSGSRAMASPSEPSLQSLDSEGRSDVSALAMILFSLLTGSDPF
                     AVTNTGEARLELPPDESLVVRSLRPELPPELDVLLARCLFSGPAERIHSISDFVYALR
                     AFNPGQGAARAERIARLVSWAESAQEEPTLLDVSAVHANQDELEEPTRLRERPEPVNR
                     SLAQVPSPSTLRPSSIPPSVGTTLYRARYRKRDRVATAAGASLLVGLVGTLWLAGKRT
                     PETQSAASAVAAGVGNGLPQSSNEPVRGESSTVPGSAAISSSRAGASTALAVSHSSLV
                     LPLAPSSAALRGVTRRPPVGRAPTVPASAANALKSTPEIPKSFGPAPNAPLDEDLILK
                     LPH"
     gene            7483..7842
                     /locus_tag="VFQ61_39195"
     CDS             7483..7842
                     /locus_tag="VFQ61_39195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960582.1"
                     /translation="MTKFSRWSGAAWVLLIWGCNAGIDAETDPASNPTAEAESAEADL
                     GSREAALTQCTDGQTKVIGCRYVCKPCRIGTCEDGHWVYESMDWGEECEPHPWPGPAT
                     CQADANGFCPAECHSCG"
     gene            complement(7933..>9618)
                     /locus_tag="VFQ61_39200"
     CDS             complement(7933..>9618)
                     /locus_tag="VFQ61_39200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibro-slime domain-containing protein"
                     /protein_id="HET9960583.1"
                     /translation="NYTFDSAAVVSGPLTRFVQPNLGNASAVVGGTSLLGKPIFTATC
                     PLSTTGTAGSWLRRNNNYYCANTVQSAATFSNWFTASAASTAVSTQLSLLRCPNPLPA
                     NHPVCTVASDADTFLFDSDTMHPDGTACSAGDLSCDGFFPLDTLPNVVKFGDCGATSP
                     NGRADQHNFHFTSEVHYWFQYDASKAATLTFTGDDDVFVFVNGRLVVDLGGIHRELQG
                     SVTLNANTLDVSGAALNLKTGEIYEIGVFQAERNTCASNYRLQLKNFVLGRSTCRPRC
                     GDGVVTPDEACDRGKDNVPATGATYGECTQACQLGPRCGDGIRQEDQGEECDNGLNND
                     LYQTTALSCATGCVKPPFCGDGKLSAVGGELCDEGADNSEQAYGPNLCSTSCQPAPYC
                     GDRSVDVDFGEGCDDGVNSGKPGSCTPDCKASVDLPSCGNGKKDAGEECDEGAKNGTR
                     GADCDARCHLRCGNGFRDPGEECDDGVNDGSYGGCNADCTPAGYCGDGVTNGPEQCDR
                     GEENGASPYGEDECTTACRKAPYCGDGRIQTEFDEECDSSPGCTNQCVTFLPR"
BASE COUNT         1734 a         3007 c         3143 g         1734 t
ORIGIN      
        1 tgaagcgcgt cactcgcggg acattgaaga aagagggcca acccggccca agctccggcg
       61 gctgcgccga gtgccgccgc tcgggttgtg gacgccaccg ggaccgatcg acggagggcc
      121 acggtaaatg cgatcagcat cagcgcgcca acggccacgg ctaccccggc gcacggcagc
      181 cccagcggat gtagctcccg agacgcgagt tgccctgctg gcgtttggag cggaccaagc
      241 gtggttacga gcgtgtacaa aggggtcacc gcaatcgccg ccgtgagcag cagcagcacc
      301 tgagcgccga gcccgcgacg acccggcgcg accactaccc ccgtcgcaat gctcgcgagc
      361 aacagcaacg cgccgagtcg ctcagtgagg ctggcccagg gaacctgtgc gagatccacg
      421 cgcagggtag ggcgtgtccg gaagaatagg tgactgccta gcggtcccgc ggcaactgcg
      481 aatgggatcg ccaccagcag ccacaaccgg cgctcgcgca agctggaacg cggcgccggt
      541 gtctgcgcca ctgcgctccg aatgcgcgca gccaactcga gcggtggcgc tagctgagtg
      601 gcgagcggat ccaaggatga gatggtttca ggcacgattg atccgatccc aaccggcgcg
      661 ggacaaagcc tctcggagcg cctcataggc acggaaggcc cgttgtttga ccgcctcgcc
      721 ggtcgttccc aggactgcgg ccgcttcggc cgcggttaga ccctccagtt tgaggaggac
      781 ataggcactg cgatttcttt cggacatgcg ctgtaactcc cgctcgacca gtcgagcgag
      841 ggcctctgca tgcaacactg attccgggga cgcatgggct tcaccggagt tctcgccggg
      901 gccctcctcc acagacgcga acaactcctc cgggcgccgg gttcgggccc gcaagcggtc
      961 caaataagtc gaccgaacga tcgcaaaggc ccagtgcagg gcgttggagc ctggcgcgta
     1021 agtccctcgg gagcggtgca gttttaggaa cgcctcctga aagaggtcgt ccgcatcacc
     1081 gccgcggccc gcgagtcgca cgcagaagga gtataaacgc gaagcgagag ccgcatacag
     1141 ctcctcgaag gcggctgcgt cgccgcccgc atagcggcgc atggcctcgt cggcattcat
     1201 tcgtttcgcg ccgggatgag tcaactcgag ggctctccgt gtgtcaaggg gaccaggcac
     1261 gcggccgcgt ctagagccga cgcagttcag tagccgagcg ggcccgtggc ggtgcgcggg
     1321 cgatgagctt gggccgctga actgaagctt cagaatgcgt tgggaaagcc tgcctgtctc
     1381 ctgctgtggg ccgatccgaa cttttttcgt taccggccac cctgcgcagc gtaactatcc
     1441 tcgtaaccgc cttcagtgcg accggcggca gatgatgcct acccggcgcg cacatggcag
     1501 tgcctgcggt gcctaaagca cacggccggc tgaaatggcc tgcgcaaacg gcgggctcga
     1561 tcggtcggct gaaactgcgg gctgaaacat ggagttcacg atgatcgtga gttggcctag
     1621 cgaggatctc gccggggagt ccttggtcca tcactgcctc gcgggtggcg agcagctgca
     1681 aagcgtaccg aactccgagg ctccttacct cgcgagcttg cccgcctcca ccgcgagtgc
     1741 gggtgaagct gtcgatccgt tcttgccggt agtcggcgcc acgatggctg ggatcgtaga
     1801 gatgctgcgc gtgttcgcgc agcaagcgga gcccatcctc atcagcggac ccacgggtgc
     1861 tggcaaatcg cgccttgcgc gttggtgtca cgaacactcg gctgcccaaa agggggcgtt
     1921 cgagatgctg gatctttcga gcgtgccaga ggagctgcag atggccgagc tcttcggctg
     1981 gagacgcggc gcctttaccg gcgcggttcg cgataatcca ggctcgatcg ctcgtgcgcg
     2041 gggcgggaca ctcttcatcg acgaggtcga caatctgtct ccgcgtgcgc aggcggggct
     2101 cttgcacgta ctcgaggatc gctcctatcg cgttctgggt gacaatggcg gcgagaagcc
     2161 ggcggaggtg cgcttcatcg tcggtacgaa cgcgcgtctc agcgaagccg tacgcgagaa
     2221 gcgctttcga gaggacctct attaccgtat aaatgtgctg ccggtgcggc tacccgcgct
     2281 ccgggaacgg gcggacgaga tccctatgtg ggcgcggtac agcgcgaatc gccatcatac
     2341 gatgcgcgtg cccgagggaa gggtcgagct ctcggcccgc gcggagctgg ccttggcgcg
     2401 cgagccatgg cctgggaaca ttcgccagct cgacaatatc atccggcggg cttacacgat
     2461 cgcattgctc gcgcatggcg gagccacgcc ccgagatctg gtgctcgagg aagatcacat
     2521 cctgcgcgcg ttggcgtacg agggttcgga gcagaggggc tcgctcgttc aagccatgct
     2581 cgaggccgcg gctgcgttcg tgagcgaggc cgagagcggg accgtggcag ggcggccgtt
     2641 ggatttggat ctagcggaca gcttcaaagg cttcgtattg ggcgttgcat ccgaacgccg
     2701 gggaagccgt gaagaggcgt tccgcttatt cggacgcgag cgactggtga cgagtcgtaa
     2761 tcatcagaag gtattcaagc gggagctcga gcgaaccgaa aatttgtgcc gcagcatcgg
     2821 caaagcagat gagttcccct tcgtgcgttg tgtggcctcg gaggaagagg cgcccaaaga
     2881 ggagacccat cccaacggct tggctcaata gcaagacgag actgcgctcg cccacaggcg
     2941 agcgagttca ctcgctcggc tctacacggt ccgccgcctc tcctcgtatt cattcattgg
     3001 tcaacgagtc ccgcgcgcag cgaaagccgt agcgtggatc ccgggtgttc ggcggcgcgc
     3061 tcgagcgctc cgcgctgcgc agccgttgcc cttggtccgc gtagctcccg ccacgcagca
     3121 cccgcacgag attggaactt cctgagtcga gttccgcaca atcgttgcag ggaaccccgt
     3181 aaggctcgcg atatacgtcg aggttccatt ctgcaacgtt gccggccagg tccgcttgcc
     3241 ccaagagccc taagcccatg ggcttcgaac ccaccggcgc gattggtacc tggtagacag
     3301 catggttcat gtccacctcg gtcagtaggg tactcggcca gggatacacc cgcgcttcgt
     3361 caccgcccgc tgctgcatag ttccactctg cttcggtggg caagcgcccg ccgtcccaag
     3421 cgcaaaatgc gaacgcttcg aaccaagtga cacagttgat gggcagctcc tcctgcccct
     3481 cgggttcgcc cgtccaggtg gaggatgagt ctgtctgcaa gttccgcggg gactcgcagc
     3541 tctcgagagc attggcgagg ccaactgcat cccgaagtag ctgggtattc cactcgtctc
     3601 tccagcccga atccgacggg ttcgatggat tgcgacccgc gccggcagcg ggtcgccatg
     3661 gatatgcggc gacgaattgt cggaaccgcc ctacggtcac ttcgtaaacg tcgagttgga
     3721 acgggctgat ctcggcgggc cacgctgtcg agtcgaaatc cccggccggg actcgatcgt
     3781 aactgcgaaa gaagcgacct cccgggacgg tcttacttgc gcagcaatcg ccggatccga
     3841 aactcgacgc gccgcacgtc gcggccaact cctggcaact gggtggcacg tcgcttgcgc
     3901 ccgcgccgcc cgtcgaaggg gtgagggtcg aacgcacccc tccagagaaa gaaaacgaat
     3961 cctgcgtgcg gccaccctga tcaggagtag ctccggagcc gccatcggtt gaactgaagg
     4021 aagagccccc ttggctcgag ggtgaccccg tagctgaatt tgcgctcgtc gagctgcccc
     4081 ctgaatgggt gctcggcacg ccagagattg cagtcgtatt cgcgccggcg gcaccaactc
     4141 ggccggcgct cgtgccgccg ccggccgagc ccgcagcggt ggacgcgccc gcgtcacgag
     4201 tgccctcctg ggaactggtg agctcaccca ctccgaagat attgctgcac gccgagctcg
     4261 cgagaacgcc gagcaacgcc ccggtttgcg cggccctcac catctgccca ccgcctgaac
     4321 accggcggat ccggggccta gaagtggcac cgcgcgaagg ctggcgtcgc gcgtttctcg
     4381 tgcgtcaggg ggagccatga accatagcgc cagggcgcct gcgatgctca ccgcgcccgc
     4441 gctgaaagcg agagtggacg ccagggcagg ggtccgagcg ctatcttgaa gttcgagagc
     4501 ctcgtcagaa caaccacgat ggccgtctgt gcagcccttt cgagcgcgat ccatgcgcga
     4561 agccaccgtg agtccgaaga tcgtgcccgc agtcactgcc ccgatcccga tgccgccgag
     4621 cgcgagcgcc caggtcttct gagtaggcca ggcgggtcgt ggcgcgggag acacgggtgg
     4681 tggtttgagc gaagcgacac ggtttggagc catgaccacg gtaatccagt agttcgtcgc
     4741 ggcctcgttc accctgactt cctgtctgaa gggtgggtgc ccgggtgctc tcgcctcgat
     4801 cacgtggggc ccaggatcga cggcgagagg gctattcaac tcggccgttg gcagcggcga
     4861 tccatcgtaa agcaaggtta cgttggggac gcgggcagtg gacggcgcga accgcacgat
     4921 aaggtgcgat atacggggct ggagctcagc agcgcgcttg tcggcgagct gcgcaacctc
     4981 tacgtcgcct gccgttcgcg cccgcgcagc cgctctcgag tagaggcttt gcgccgtggc
     5041 ggcgttccgg gatagctcat gacagagcgc tagtctcaac agcacacccg tgcctggatg
     5101 taaccgaaag ctctccgcga gcctcgggca agcttcagac aagcgccgct gattcagaag
     5161 cgccgtgccc tcctcgtcca gggcggcggc tcgcgtggaa tcatcactgc tccgaggcag
     5221 ctcgctcggc tcgggtggcg tcttcgccgc gtcaagagcg gtctgggccg tgatgttcgt
     5281 cggcgcctgt tctcgcttct gcgcctgcgc tgtcggtagc cagagcgcag gtacgagtag
     5341 ggtcacgggt cgccatcctc ttcgggatag aacgagcaat tgagcgtacg aatacatagt
     5401 cagtggagaa gccgtattag gagctggcga aaggtcttca gtgtggaagt ttgaggatca
     5461 aatcttcatc caagggggcg ttgggtgcgg ggccaaagga cttcgggatt tcaggtgtgg
     5521 atttcagcgc attcgctgct gacgcgggaa cggtaggtgc gcggccgacg ggggggcgtc
     5581 tcgtaacacc acgcagggct gccgagctcg gtgcgagcgg gagcaccaaa ctcgaatgtg
     5641 aaacggccaa cgcggtagac gccccggctc tcgaagaaga gatagcggcc gaacctggca
     5701 ccgtcgagct ctcgcctcgg actggttcgt tcgaagattg cggcaaccca tttcccaccc
     5761 ctgcggctac ggcgcttgcg gcgctctgcg tttcgggagt ccgtttacca gcgagccaga
     5821 gtgtaccgac taggccgacc agtagcgaag cgccggcagc cgtagccact cgatcccgtt
     5881 tgcgatagcg cgcccggtaa agcgtggtcc ccacggacgg gggaatcgag gagggtcgga
     5941 gcgttgaagg cgagggcacc tgcgccaggc ttcggttcac gggttccggc cgttcccgta
     6001 gtcgcgtggg ttcttcgagt tcatcctggt tggcatgaac cgctgagacg tccagcaacg
     6061 tgggttcctc ctgggcgctc tcggcccagc ttacgagccg cgcgattcgc tcggctcggg
     6121 cggcgccttg tcccgggttg aaggcacgga gagcgtagac gaagtccgag atcgaatgga
     6181 tgcgttccgc cggaccagag aacaggcaac gagcgagaag tacgtcgagc tcaggcggca
     6241 gttcgggacg cagtgaacgc accacgagtg actcatcggg ggggagctct aggcgtgctt
     6301 caccagtgtt cgttactgcg aacggatcac taccggtaag cagtgaaaac aggatcatcg
     6361 ccagcgcact cacatcgctg cgcccttcgg aatccaagct ctgaaggctc ggctcggacg
     6421 gcgaagccat ggcgcggctt ccgctgtcgc cgcgcgagac gttgggcctc ttgtcgcatt
     6481 gacccagccc gaaatccaac accttgatcg acggcgtgcc gtccagtccg cgcgtcaaaa
     6541 acagatccga gggctggagc gctcgacggc caattccgag cgcttgggct tccgcgagcg
     6601 gctcgcaggc ttgcgtgatg taatcgactg caacggtgag cggtaagggg ccatcctttc
     6661 gaagcacggc gctcaggtct cgacccggca ggtgttcgta gatcacgaac acagagccgt
     6721 ctccgagcgt gcctgcatcg agtacacgag cggcgtgctc gcttcgaagc tgtgcggccg
     6781 cgcgcacgtc ggtcaacacg cgctgcaaga agagcgcgtc tttgggggta gtcggccaga
     6841 agaacttgag ggctactagg cggttgaggg cgacgtgccg cgcggtagcg acgatcccgc
     6901 acgcactgcg ctcggcaatg cgttcgatga cgtacctccc gtcgaccact tctccgattg
     6961 gaatggttcg catcccggct cctgctttgc ggagcccgca tgcacgcgat ctcctgcctg
     7021 agcccctcgc acgtcccgtg ccgcgcagtt tcatcgataa ctatagggac tgcgcttatc
     7081 ggcccgtgtt cggagcgtga atctcggacg catatcgctc gctatcgtac gctgctcgct
     7141 ccgggctcac cctctggacc gaacacggct gcgctaccag ggtgcggagg gtcttgggag
     7201 gggctctctc gaacgatcac tttcgacctg gttcgcgcat gaaaccgagt accaggcttc
     7261 tccgtccgtt gttggcgact gtgccggttc gcctcacgtg agcggctccg tcatgcgcgc
     7321 gggttcggag cggtgcacga gcgcgccact ccgcaaaatc acgccaccac atgaccatgg
     7381 atcgtcgcgt cgtgaagccc gatactccga gcgtccattc attcgttgga tcgttgatcg
     7441 ttggatgcac gggctgccgc cgtgtgcggc aaggaggata cgatgacgaa gttctctcga
     7501 tggtctggcg cggcttgggt gttgctgatc tggggctgca atgcgggcat cgacgccgag
     7561 acggatcccg cgtccaatcc cacggcggag gccgaatcgg cggaggcgga cttgggctcg
     7621 cgagaggccg cgctgaccca atgcacggat gggcaaacca aagtcattgg ttgccgctac
     7681 gtctgtaagc cctgccgaat cgggacctgc gaagacggac actgggtcta cgagtcgatg
     7741 gattggggcg aagagtgcga gccgcatccg tggccaggac ccgcgacttg ccaagccgat
     7801 gcgaatgggt tctgcccggc agaatgccat tcatgcggtt gatttgcggt tggctcgcca
     7861 tggagcggtg agccgccaat gctgcgcgcc cgtgagaagc gggcgcgcag caggcactgt
     7921 cagccctgag acttaccggg gcaggaaagt cacgcactga ttcgtgcagc cggggctgct
     7981 gtcgcactcc tcgtcgaact cggtttggat gcggccgtca ccgcaatacg gagcctttcg
     8041 gcaggccgtc gtgcactcgt cttcgccata ggggctcgcg ccgttttcct cgccgcgatc
     8101 gcattgttct gggccattgg tgacgccatc tccgcagtac ccggcgggcg tgcagtcggc
     8161 attgcacccg ccataagagc cgtcattgac gccgtcgtcg cattcctcgc cgggatcccg
     8221 gaagccgttg ccgcaacgca agtgacaacg agcgtcgcag tccgcgcctc gagtgccgtt
     8281 ctttgcgcct tcatcgcact cctcgcccgc gtccttcttg ccattgccgc aagagggcag
     8341 gtccacgctc gccttgcagt cgggcgtgca ggagccgggt tttccactgt ttacgccgtc
     8401 atcgcaccct tcgccgaagt cgacgtctac gctgcgatcc ccacaatacg gcgcaggctg
     8461 gcagctggtc gaacaaagat tggggccgta agcctgctcc gagttgtcgg cgccctcgtc
     8521 acagagctcg ccacctaccg cgctcagctt gccatcgcca cagaacggcg gcttcacaca
     8581 accggtcgcg caagagagag cggtggtctg atagaggtcg ttgttcagac cattgtcaca
     8641 ctcttcgcct tgatcctctt gtcggatgcc atcaccgcag cgcggtccga gttggcatgc
     8701 ctgggtgcac tcaccgtaag tcgcccccgt cgcgggcacg ttgtccttgc cacgatcgca
     8761 agcttcgtcg ggcgtaacca cgccatctcc gcaacgaggt cgacatgtcg accgacccag
     8821 gacgaagttc ttgagttgca ggcgatagtt cgatgcgcag gtgttgcgtt cggcctggaa
     8881 cacaccaatt tcgtagatct ctccggtctt caggttcagc gcggcgccac tgacgtccag
     8941 tgtgttcgcg tttagcgtga ccgagccttg gagttcgcgg tgaatgccgc ccagatccac
     9001 gaccaggcgt ccattcacga acacgaatac gtcgtcatca cccgtgaacg tgagtgtcgc
     9061 ggccttgctc gcgtcgtact ggaaccagta gtgcacctcg ctcgtgaaat ggaaattgtg
     9121 ctgatcggcg cggccattgg gcgaggtcgc gccgcagtcg ccgaacttga ccacgttggg
     9181 tagcgtgtcg agcgggaaaa aaccgtcgca actcaaatca cccgccgaac aagcggtgcc
     9241 gtccgggtgc atcgtgtcgc tgtcgaacag gaacgtatcc gcgtcgctgg ccacggtgca
     9301 caccggatga ttggcgggta gcggattcgg gcaccgaagc agcgagagct gcgtcgagac
     9361 tgcggtggac gcggcgctcg cggtaaacca attcgagaac gtggcggcgc tctggacggt
     9421 attggcacaa taatagttgt tgttgcgtct tagccagctg cccgccgtgc cggtggtcga
     9481 aagtgggcac gtcgccgtga agattggctt tcctaacagc gacgtcccac ccaccaccgc
     9541 agaagcgttg ccaagatttg gctgcacgaa gcgcgtcagc ggcccagaaa cgactgctgc
     9601 ggagtcgaac gtgtagtt
//
LOCUS       DASTKU010001097         4721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_439733, whole genome shotgun sequence.
ACCESSION   DASTKU010001097 DASTKU010000000
VERSION     DASTKU010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439733"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2872
                     /locus_tag="VFQ61_39205"
     CDS             <1..2872
                     /locus_tag="VFQ61_39205"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9960584.1"
                     /translation="ADRPVRVGELDEEMVFETQVGETFTLGASTWRIEEITHDRVLVS
                     PAPGQPGKMPFWKGGGPGRPVELGRRIGALTRELASLPLEKASLRLREQHGFDPGAVE
                     SLIQYLHEQREATAVVPSDRAILIERFKDELGDYRVCVLSPFGRRVHAPWAMALSERF
                     RASAGAEIDAVWSDDGIVLRLPETEEQLATEHFVPDPEEVEALVVQGLAKSSLFAAHF
                     RENAGRALLLPKRDPRKRMPLWAQRRRAADLLRVASQYPSFPILLETYRECLRDVFDL
                     PALIELLSAIRRREVELVPIESRSPSPFASTILFSYVGNFLYDGDAPLAERRAQALVL
                     DQAELRELLGEAELRELFDADTIASVERDLLRLHEPTLRHADDIHDVLLLLGDLTEED
                     LKARAKNPDTLSESLQQLGAARRIARAAIAGEPRYFAAEDAGKLRDALGVMPPAGLPS
                     TFLAPVPKALEELLARYARTHAPFTGDAPARRYGLGRSVVDAALAALEQSGRVVRGEF
                     MPGGREREWCDKNVLRMIKQRSLAKLRKAIEPAPPEAYARLSLLWQGAHEPSRASDAL
                     DRAIDQLQGAPIAASVLVQNVLPARLRGFTPAALDALLAMGEVTWVGVEPHGQSDGKI
                     ALYRSEKLALLAPPVTPVTSELAARLRVELGQGGALFFQDLVRRIGGFPRDLLKALWD
                     LVWAGEVTNDTLAPLRSLLTEQRSSRGRPDPRARRPQLPGSEGRWSLVRREEAGSVAE
                     QRMARAAVLLDRYGIVSREIVNADGLGSFADLYPVFKAMEDAGKLRRGYFIAGLGGAQ
                     FARPGADERLRQKPEPGSRAVVLAATDPANPYGAALSWPESEPRPVRTPGALVVLHEG
                     RLLAYLAKSDRSLTLFLSEEEPDRSQGLEAVLDALKTLVGPGKRRALLIQTLNGREAT
                     RSELATAFIGAGFSSGASGLGLRRSGVSAADLESEALEDAGG"
     gene            2859..3686
                     /locus_tag="VFQ61_39210"
     CDS             2859..3686
                     /locus_tag="VFQ61_39210"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET9960585.1"
                     /translation="MPEGDTLFQAAVRLHAALAGQVITRFATPVPALKEQGLVGQRVE
                     RVRSQGKYVIVEFGDGRALLTHLRMQGKWSVLRKADLRASARSSRDPRWDDDRTTLIL
                     ETAEIRAILERAAVAELAPLREIERQLASLGPDMMAPEFDAVAAREALKTHATQTIAE
                     ALMVQSLVAGIGNVYKSELLFLEKQNPFQPVSALDDAALDRLLRRARELMLRNRKGPR
                     RTTFGTFASTPYYVYERSGQHCIKCDSKIRMRRQGPLQRSTYYCPECQTVPDEPPAR"
     gene            complement(3728..4183)
                     /locus_tag="VFQ61_39215"
     CDS             complement(3728..4183)
                     /locus_tag="VFQ61_39215"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET9960586.1"
                     /translation="MQERLSSSRRRFLKVVGAGSMLGAIPSCGDSSSPPEAFADVSAG
                     KVADTAVGTLRAIPGAPAYLGRDQQGLYAMTSTCPHEGCDMIAEGSVSADGVYCACHG
                     SRFDANGAVLAGPANSPLVHFAVDLDGDGNITVHGGSQVDASVRKTVVG"
     gene            complement(4243..4680)
                     /locus_tag="VFQ61_39220"
     CDS             complement(4243..4680)
                     /locus_tag="VFQ61_39220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HET9960587.1"
                     /translation="MFDLDRFEKAQDPVIETVLRELRAGHKQTHWMWFVFPQLRGLGY
                     SQMARHYGIESGAEARAYLEHAVLGARLEACTQCVLAVTGRSAHEIFGSPDDLKLRSC
                     MTLFAVVAPERRIFAQVIDRYFAGESDEKTLELLKSTEMNASR"
BASE COUNT          849 a         1538 c         1518 g          816 t
ORIGIN      
        1 cgcggatcga ccggtgcggg tcggtgagct ggacgaggag atggtcttcg agacgcaagt
       61 cggcgaaacg ttcacgctcg gggcgtcgac ctggcgaatc gaagaaatca cgcacgatcg
      121 cgtgctggtg agcccggcgc ccggtcagcc aggaaagatg ccgttttgga aggggggtgg
      181 cccgggtcgc ccggtcgagc tcgggcgacg catcggcgcg ttgacccgag agctcgcttc
      241 tctgccgttg gagaaggcga gtttgcggct acgtgaacag cacggcttcg accccggcgc
      301 ggtcgaaagt ttgattcaat atctgcacga gcaacgggaa gccacggccg tggtgccgag
      361 tgatcgtgcc atcttgatcg agcgcttcaa ggacgaactc ggagactatc gagtgtgcgt
      421 actgagcccc ttcggtcggc gcgtgcacgc gccctgggcc atggcgctca gcgaacgctt
      481 ccgcgccagc gcaggcgccg aaatagacgc ggtctggtcg gacgatggca tcgtgcttcg
      541 attaccggaa accgaagagc agttggccac cgaacatttc gtgccagatc cggaggaggt
      601 ggaagccttg gtggtgcagg gcttggccaa gagctcgcta ttcgcggcgc atttccgcga
      661 gaacgccggg cgcgcgctgc tgcttccgaa gcgggaccct cgcaaacgca tgccgctctg
      721 ggcgcagcgc cggcgcgcgg cggacttgct gcgggtggcg agccaatatc catccttccc
      781 aatcttgctc gagacctatc gagagtgctt gcgagatgtt ttcgacttac cagccctgat
      841 cgagttgctg tccgcgattc ggcggcgcga agtcgagctc gtcccgatcg agtcgcgtag
      901 cccttcgccg ttcgcgagta cgatcctctt ctcgtatgtc ggcaattttt tatacgacgg
      961 ggacgcgccg ctcgccgaac gccgagcgca ggccttggtg ctggaccaag cagagctgcg
     1021 tgagctgctc ggagaggcgg agctgcgcga gctattcgac gcggacacca tcgccagcgt
     1081 cgaacgcgac ttactgcgac tgcacgaacc cacactgcgg cacgcggacg acatccacga
     1141 cgtgctcttg ttactgggcg atctgaccga agaagacttg aaggcgcgag ccaagaaccc
     1201 ggacaccctg tccgagtcgt tgcagcagct cggcgcagcg cgacgtatcg cccgtgctgc
     1261 gattgccggt gagccgcgct acttcgccgc cgaagacgcg ggcaagctgc gagatgcgct
     1321 cggcgtgatg ccccctgccg gtttaccgag caccttcctc gcgcctgtgc cgaaggcgct
     1381 ggaggagctg ctcgcgcgct acgcccgaac gcacgcgccg ttcactggtg atgcccccgc
     1441 gcgtcggtac ggcctcggtc gcagcgtcgt cgatgccgcg ctcgccgccc tcgagcagag
     1501 cgggcgagtg gtgcgcggcg agttcatgcc cggtggacgc gaacgcgagt ggtgcgacaa
     1561 gaacgtgctg cgcatgatca agcagcgcag cttggcaaag ctacgcaaag ccatcgagcc
     1621 ggcgccgccc gaagcgtacg cgcgcctgag cttactttgg caaggcgcgc acgagccctc
     1681 ccgcgccagc gacgcgctcg atcgagcaat cgatcagctg caaggcgcgc cgattgcggc
     1741 ctcggtgttg gttcaaaacg tgttgccggc ccggctgcgc ggcttcactc cggcggcgct
     1801 cgacgcgctg ctcgcgatgg gggaagtgac ctgggtcggt gtcgaacctc acggccagag
     1861 tgacggaaag atcgcgcttt atcggagcga aaagctagcc ttgttggcgc ccccggtcac
     1921 acccgtgact agcgagctcg cggctcggct gcgcgtggag ttgggccagg ggggcgcgct
     1981 attctttcaa gacctggtac gccgcatcgg cggcttcccg cgggacctac tcaaggccct
     2041 gtgggaccta gtctgggccg gcgaggtcac caatgatacg ctggctccgc tccgctcgtt
     2101 actcaccgaa cagcgcagct cgcgaggtcg ccccgatccg cgcgcgcggc gcccgcaatt
     2161 gccaggcagc gaaggccgct ggtcactggt gcgccgagag gaagcgggca gtgtggccga
     2221 gcagcgcatg gctcgcgccg cggtgttgct ggatcgctac ggcatcgtca gtcgggaaat
     2281 cgtcaatgcc gacggcctcg gcagttttgc agacttgtac cccgtcttca aagccatgga
     2341 agacgcgggc aaactccgcc gaggttactt catcgcgggg ctaggaggcg ctcagtttgc
     2401 ccgacccggc gcggacgaac gccttcgaca aaagcccgaa cccgggtcac gcgcggtggt
     2461 tctggcagcc accgatcctg cgaacccgta cggggcggct ttatcatggc ccgagagcga
     2521 gccgcgcccc gtccgtactc ccggggcatt ggtcgtctta cacgaggggc gtctgctcgc
     2581 ctatttggcc aagagcgatc gctccctcac actgttcctc agcgaggaag agccggaccg
     2641 cagccagggc ttggaagccg tgttggacgc cctgaagacc ttggtcggtc cgggcaagcg
     2701 tcgggccctg ttgatccaga ctctcaatgg acgagaggcg acccgttccg agcttgcaac
     2761 cgcgttcatc ggcgcgggct tctcgtcggg tgcatccggc ttggggctgc ggcgcagtgg
     2821 cgtgagcgcc gccgatctgg aaagtgaggc gctggaagat gccggagggt gataccttat
     2881 ttcaagctgc cgttcgcttg catgctgcgc tcgcgggaca ggtcatcacc cgcttcgcca
     2941 ccccggttcc cgcgctgaag gagcaaggac tcgtggggca gcgtgtggag cgcgtccgtt
     3001 ctcagggcaa atacgtgatc gtggagttcg gagacggccg cgcgctgctc actcatctgc
     3061 gaatgcaggg taagtggtcg gtgctccgca aagccgattt gcgagccagc gcacgatcga
     3121 gtcgtgaccc gcgctgggac gacgacagaa ccacgttgat cctagaaacc gcggaaatcc
     3181 gcgcgatttt ggagcgtgcc gccgtcgcgg agctcgcccc gctacgggaa atcgagcggc
     3241 agctcgcaag cctcggcccg gacatgatgg cccccgagtt cgacgcggtg gccgcacgcg
     3301 aggccctgaa aacgcatgcc acgcagacca tcgccgaggc gttgatggtg caatcgttgg
     3361 tggcggggat tggcaacgtc tacaagtccg agctgctgtt tctggaaaag cagaacccgt
     3421 tccaacccgt ctccgcgctg gatgatgcgg cattggatcg gctgctccgc cgcgcgcgcg
     3481 agctgatgtt gcgcaatcgc aaaggcccgc gtcgcacgac cttcggtacc ttcgcgagca
     3541 cgccatatta cgtttacgag cgcagtggcc agcactgcat taaatgcgat tccaagatcc
     3601 gcatgcggcg ccagggcccc ctccaacgct cgacctatta ctgccccgag tgccaaacgg
     3661 tgcctgacga acccccggcg cgctgagccg cttcatttcg gagcggcgcc tagctcggag
     3721 cgggcgccta gccgaccacg gtcttacgca cggaggcgtc cacctggctg ccgccgtgta
     3781 ccgtgatgtt accgtcccca tccagatcga ccgcgaagtg caccagcggc gaattcgccg
     3841 gccccgcaag tacagcgccg ttcgcatcga aacggctgcc gtggcacgcg caatataccc
     3901 cgtcggcgct gaccgaacct tcagcgatca tatcgcagcc ctcatgagga caggtggagg
     3961 tcatcgcata caagccttgt tgatcgcggc ccagataggc gggagcgcca gggatcgcgc
     4021 ggagcgtacc cacggccgta tccgccacct tgcccgcgct cacatccgca aaagcttccg
     4081 gaggagagga ggaatcaccg cagctcggga tggccccgag cattgatccc gcacccacca
     4141 ccttcaaaaa ccgccgccgc gaagaactga gtctttcttg catgacattc ctcacttggt
     4201 cccaacctac gtaccagacc aatcggcgcc cagagcgcct tgttaacggc tagcgttcat
     4261 ttcggtgctc ttgagcagct ccaacgtctt ctcgtcactt tcaccggcaa agtaacggtc
     4321 gatcacctgt gcgaaaattc gcctctccgg cgccaccacg gcgaacaacg tcatgcacga
     4381 tcgcagcttc agatcgtcgg gcgatccgaa gatctcgtgt gcacttcggc ccgttaccgc
     4441 cagcacgcac tgcgtgcacg cctcgagccg cgcccccaac acggcgtgtt cgaggtaggc
     4501 gcgcgcctcg gctccggatt cgatgccgta gtggcgcgcc atctgactgt aaccgagccc
     4561 tctcaactga ggaaacacga accacatcca gtgggtctgc ttgtggccag cgcgaagttc
     4621 ccggagcacc gtctcgatga cggggtcctg ggccttctca aaccgatcga gatcaaacac
     4681 cgcactgccc cgctacgcca cgaaaacccg gaaagtcgcc a
//
LOCUS       DASTKU010001098         9670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_886941, whole genome shotgun sequence.
ACCESSION   DASTKU010001098 DASTKU010000000
VERSION     DASTKU010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9670
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886941"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1847)
                     /locus_tag="VFQ61_39225"
     CDS             complement(<1..1847)
                     /locus_tag="VFQ61_39225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET9960588.1"
                     /translation="MKALSAGLKTAKDLLLRRDGPLTRELLLEPGKFGLGRVPKRLAA
                     EQTTTMVCGFCSTGCGLNVWLKDGQAVGLTPSTEYPVNMGMACPKGWEALTPLVSSGR
                     ASHPMLKRHGKLSPVSWEEALSEMVERFRAIQEKHGKGAIAWLGTGQMPTEELAFLGA
                     LGKFGIGIQHGDGNTRQCMATAAVAYKQAFGFDAPPFTYKDFEESDVIVLIGSNLCIA
                     HPIMWERICKNPHSPSIVVVDPRKTETAMASTHHYAILPKSDLSLLYAVANILIENGW
                     IDQAFIAEHTSGFAEFKAHVAGFSKERAARESGISEDRIEALARLIHEGKRVSFWWTM
                     GVNQSHQGVRTAQAIIALALMTRNIGRPGTGANSITGQCNAMGSRLFSNTTNLLGGRD
                     FANAQHRADVAEILGIDPEQIPSEPSLAYDQIVEGILAGKIKALWVIATNPAHSWINQ
                     AHLHEVLGRLECLVVQDMYHDTETAQRADILLPAAGWGEKEGTFINSERRVGVLKKVA
                     RAPGQALADFHIFQLVAAAFGVGDMFREWSSPEAVFQILKRLSRGRPCDITGIGDYVD
                     VDRAGGIQWPLPEGQSVEETERRLFEDGEFYTEDKRAKFLFAEPAPLPEA"
     gene            complement(1844..3574)
                     /locus_tag="VFQ61_39230"
     CDS             complement(1844..3574)
                     /locus_tag="VFQ61_39230"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET9960589.1"
                     /translation="MSVAATHLEVSTGEPLPTGEAAKADGKSSPRRLPLLPVAALLQA
                     QQTLTAVERFSRVHEEGGVPAQAKYYRDLIPLERPRPGEQYAFEVDLDACTGCKACVS
                     ACHTLNGLDENEVWRTVGFLHGGTSQAPALQTVTTSCHHCLEPACMSGCPTQAYEKDP
                     VTGIVKHLDDQCFGCQYCTLMCPYDAPKYNKSLGIVRKCDMCSSRLEHGEAPACVQAC
                     PNEAIAIRVVERQAAIEASNAQSFLPGAPAPDHTLPSTVYKTERVMPANMLPADFYRT
                     SPEHSHPPLVIMLTLTQLSVGAFALSLLVERLSGHPAGSPLAQTAFACALALVALFAS
                     VFHLGRPLLAYRAFLGLRTSWLSREAVAFGVFAKLAILYGVLAAAPALPEFPGRASLL
                     AASGSLQMAAAAVGIMGVFFSVMVYVATRRVQWSGTRTGIKFFGTMLLLGAASVLGVS
                     MATRGAELALGRASDALLVLVFAVTLVKLSFEAAILAQIRDRRLSVERRMAALMLGEL
                     RSATALRFGLAIVGGLLLPVAFFVQWIPNSASGPAASAMLAALLGAELSERYLFFRAA
                     PASRMPGGLR"
     gene            complement(3571..3957)
                     /gene="nirD"
                     /locus_tag="VFQ61_39235"
     CDS             complement(3571..3957)
                     /gene="nirD"
                     /locus_tag="VFQ61_39235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HET9960590.1"
                     /translation="MNNSFVESEPQRMHGMPYYNACSLSDVLPGTGVAVMIAGQQIAI
                     IRTRDTGQVYALSNFDPFSKAFVIARGIVGDRGGIPKVASPIFKQSFDLRTGRCLDDP
                     NVSLPVYPVRVVAGRIEVQVPPLVEA"
     gene            complement(3954..6551)
                     /gene="nirB"
                     /locus_tag="VFQ61_39240"
     CDS             complement(3954..6551)
                     /gene="nirB"
                     /locus_tag="VFQ61_39240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HET9960591.1"
                     /translation="MGSLTNGKSKPRLVVVGNGMVGHRFLEELIRRGLTAKYEVVCFG
                     EEPRVAYDRVNLSKYFEGKSAEDLALGSAEQYRSAGIDLRLDARVSELDLAARALTVV
                     SGDQSEQLNYDWLVLATGSRPFVPEIPGNKSARCFVYRTIEDLRAIQDCARECKSGVV
                     IGGGLLGLEAANALRQLGLKTHVVEFASRLMPLQIDDVGGAILRGRIEELGVSVHTSR
                     ATTRIEVNAEGRAEALSFSDGNELRVDMVVFSAGIRARDELARAAGLKIGERGGIAID
                     VGCQTSDPRVFAIGECAAFSGKTYGLVAPGYRMAEVAAARIAGEEANFVDADMSTKLK
                     LLGVDVGSFGDAFARTPGARVISLYDNVAGVYKKLVLSEDKKQLLGGILVGDASAYTE
                     LTTYVQNSLPLPEHPEDLIVPPREGGKKGLGVDALPDAAIICSCHNVDKGSIVNLIRD
                     EKLVELSAVKKCSKAGTGCGSCSTLVDDVLKCELKRAGIRVNNHICEHFKYSRQDLYQ
                     LVRLHEIRSFDELLARHGQGQGCEICKPAVASILASTWNDYILRAEHAGLQDTNDRFL
                     ANIQRDGTYSVVPRVAAGEITPDQLIAIGRVAKKYGLYTKITGAQRIDLFGARLEQLP
                     SIWRELIAAGFESGHAYGKALRTVKSCVGSTWCRYGVQDSVGLALKLENRYKGLRAPH
                     KLKGAVSGCARECAEAQGKDFGVIATEKGYNLYLCGNGGMKPQHAVLFAADIDEDTVI
                     RYLDRFLMFYVKTADRLQRTATWLNKLEGGIEYVKQVVIEDSLGIGAELEAQMQHVVD
                     TYQCEWKTTVQDPEKLKMFRAFVNTDDSDPSIVWVKEREQHRPAFWDEKLPLVESGKI
                     RLPVVQVAP"
     gene            complement(6838..8253)
                     /locus_tag="VFQ61_39245"
     CDS             complement(6838..8253)
                     /locus_tag="VFQ61_39245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015514 - nitrite efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015707 - nitrite transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HET9960592.1"
                     /translation="MSKASSDTASVGKASVGRDGKYWISDWNPEDPKFWAERGKPIAR
                     RNLIWSILAENVGFSVWLVWSVVATRLPKAGFPYTTEQLFQLVAVPGLVGALARIPYT
                     LAVPKFGGRNWTIVSALLLSIPTLSMIVLVRDPSTPFWLMLLSAASAGLGGGNFASSM
                     ANISFFFPDREKGFALGLNAAGGNIGVSTVQLLVPIVVGYALLPGGTSKIHLENAGLM
                     WLPLILLAVLGATFFMNNLTTARSNVKDQVVVTQERHTWIIAWLYIGTFGSFVGYSAA
                     FPLLLKTQFPEVTTNLAFLGALVGSVARPLGGKIADRLGGARVTFWNFVGMAVASLGL
                     LWSLETHNLSAFLVVFLMLFMTTGIGNGSTFRMIPVIFRTLHLRRARGRGEEAEAAAF
                     VAARRETAAVIGIASATGALGGYFIPRAFGASIQATGGASGAVRYFLAFYVTCVGLTW
                     WCYLRSSFLVRRVPSLAEAGA"
     gene            complement(8699..>9670)
                     /locus_tag="VFQ61_39250"
     CDS             complement(8699..>9670)
                     /locus_tag="VFQ61_39250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HET9960593.1"
                     /translation="RAEVGDDVYGEDPTTRRLEERVAQLLGKEAALFVPSGTMSNQIA
                     LQVHTRRGDEVVVAEAAHVVNDESGAGAAWSGVQFTSVGAEGAFTVQDLERAVRPISD
                     FVPRTALVWIENTHNRSGGRVFPQTEILGLAVRARELGLALHLDGARLWNAAVASRTS
                     EQELAAPFDTVSVCFSKGLGAPVGSALVGRKQEIDRARRFRKMLGGGMRQSGVLAAAA
                     LYAVEHHRERLALDHEAALRLAQVIGEAPGVTVKHPETNIVVAELAQPVARAVVLECE
                     RAGLLLYALSPTTLRLVTHLDLGADDIQAAARVLLGVLDRMASNPSA"
BASE COUNT         1933 a         3148 c         2831 g         1758 t
ORIGIN      
        1 gcttctggaa gcggcgccgg ctcggcgaac agaaacttgg cgcgcttgtc ctcggtgtag
       61 aactcaccgt cctcgaacag acgccgctcg gtttcctcga ccgattgccc ctcgggcagc
      121 ggccactgga ttccaccagc gcgatcgaca tccacgtagt ctccgatgcc cgtgatatcg
      181 caaggtcgac cgcggctcag ccgtttgaga atctgaaaca cggcttcggg ggaagaccat
      241 tcacggaaca tgtcgccgac gccgaacgcg gcggcgacga gctggaagat gtggaaatcg
      301 gctagtgctt gcccaggtgc ccgagcgacc ttcttcaata cgccgacgcg acgctccgag
      361 ttgatgaacg tgccctcttt ctcaccccac cctgcagcgg gcaacaagat atccgcgcgc
      421 tgcgccgtct cggtatcgtg atacatgtct tgcaccacta gacactcgag ccgccccagg
      481 acctcgtgca aatgggcctg gttgatccaa gagtgagcgg gattggtcgc aatcacccag
      541 agcgccttga tcttgccggc caagatccct tccacgatct ggtcatacgc gaggctcggc
      601 tcactcggga tctgctccgg atctatcccc aagatttcgg cgacgtctgc gcgatgctgg
      661 gcattggcga agtccctacc gccgagcaga ttcgtggtgt tgctgaaaag gcgcgagccc
      721 atggcattgc actggccggt gatggagttc gcgccggtgc ccggccgccc aatgttccgg
      781 gtcatcaacg cgagcgcgat gatggcttgc gcggttctca ccccttggtg gctctggttt
      841 acccccatcg tccaccagaa gctcactcgc tttccttcgt ggatcaaacg tgcgagcgcc
      901 tcgatgcggt cctcggagat cccgctctcg cgtgcagccc gctccttgct gaagccggcg
      961 acgtgagcct tgaactcagc gaagccgctc gtgtgttccg cgatgaacgc ctgatcgatc
     1021 cagccgttct cgatcaagat attggcgacc gcgtacagca aggaaaggtc gctcttgggc
     1081 aggatggcgt agtgatgcgt ggacgccatc gccgtttccg tcttgcgcgg gtcgaccact
     1141 acgatcgacg gagagtgcgg gttcttgcaa atgcgctccc acatgatcgg gtgggcgata
     1201 cacaggttcg acccaatcag gacgatgacg tcggattctt cgaaatcctt gtacgtgaag
     1261 ggcggagcat cgaagccgaa agcttgcttg taggcgacgg ccgccgttgc catacactgc
     1321 cgggtgttgc catcaccgtg ctgaataccg attccgaact taccgagcgc gccgagaaac
     1381 gcgagctcct ccgtcggcat ttggccagta ccgagccacg cgatggcgcc cttaccgtgc
     1441 ttctcttgaa tggcgcggaa gcgctcgacc atctcgctga gcgcttcctc ccagctcacg
     1501 gggctgagct ttccgtggcg cttgagcatg gggtggctgg ctcgcccgct cgaaaccagc
     1561 ggcgtgagcg cctcccaacc cttcgggcag gccatgccca tgttgacggg atactccgtg
     1621 gaaggcgtga gcccgaccgc ttgaccgtcc tttagccata cgttcaggcc gcacccggtg
     1681 gagcaaaagc cgcaaaccat ggtggtggtt tgctctgcgg ccaatcgctt gggcacgcgc
     1741 cctagcccga acttgccagg ttccagcagc agttcgcgcg tcaacgggcc atcccggcgc
     1801 agcagtagat ccttggctgt cttcagtccc gcactcagtg ccttcatcgc agtcctccag
     1861 gcatgcgcga ggcaggagcc gcgcggaaaa acaagtagcg ctcgctcaac tccgccccca
     1921 acaaggcagc cagcatggcg ctcgcggcgg ggccactcgc cgagttcgga atccactgaa
     1981 cgaagaatgc gactggcagc agcagccccc ccacgatcgc cagaccgaaa cgcaacgcgg
     2041 ttgcggagcg caactcaccc aacatcaatg ccgccattcg ccgctcgacc gacaggcgtc
     2101 gatcccggat ctgcgccaag atagcggcct cgaagctcag tttgacgagc gtcacggcga
     2161 agaccaacac cagcaacgca tctgacgcgc gacccaaggc gagctccgcg cctcgagtcg
     2221 ccatactgac cccgagtacc gaagcggccc cgagcagcaa catagttcca aagaatttga
     2281 ttcccgtgcg cgttcccgac cattgcaccc gccgcgtcgc cacgtagacc atcaccgaga
     2341 agaacacgcc catgatgccg accgcggcgg ccgccatctg caagctcccc gacgccgcga
     2401 gcagggacgc gcgccccggg aactctggca acgccggagc ggctgccaac actccgtaca
     2461 gaatggcgag cttcgcgaac acgccaaaag caacggcctc tcggctcaac caggaggtcc
     2521 gaagccccaa gaacgcccga taagcgagca gcggccggcc gaggtgaaac acgctcgcga
     2581 acaacgcaac cagcgcgagc gcgcatgcga acgcggtctg cgcgagtggg ctccccgccg
     2641 gatgccccga tagacgctct accagcagac tcaacgcgaa cgcacccacc gagagctggg
     2701 tgagagtcag catgatcacg agtggcggat gcgaatgctc cgggctcgtc cgatagaagt
     2761 ctgccggcaa catgttggcc ggcatcacgc gctccgtctt gtaaaccgtc gaaggcaggg
     2821 tgtgatccgg cgccggagcg cccggcagga agctctgcgc gttgctcgcc tcaatcgcgg
     2881 cttgccgctc caccacccga atcgcgatcg cttcgttcgg gcaggcttga acgcaggccg
     2941 gagcctcgcc atgttccagc cggctgctgc acatgtcgca cttgcgcacg atgcccaaag
     3001 acttgttgta cttgggcgcg tcgtacgggc acatcaaagt gcagtactgg cagccgaagc
     3061 attgatcatc caagtgcttg acgattcccg taaccggatc cttctcgtac gcctgagtgg
     3121 ggcagcccga catacaggcc ggctccaaac aatgatgaca agaagtggtc acggtttgga
     3181 gcgcaggcgc ctgcgaggtt cccccgtgga ggaaccccac cgtgcgccag acttcgtttt
     3241 cgtccagacc attgagcgta tgacacgccg aaacacaggc cttgcagccc gtgcaagcgt
     3301 ccagatccac ctcgaacgcg tactgctctc ccggccgagg ccgctcgagc gggatgaggt
     3361 cccggtagta cttcgcctga gcgggcactc cgccctcttc gtgaacgcgc gagaagcgct
     3421 ccaccgccgt gagcgtctgc tgcgcctgca gtaacgccgc gacgggaagc agaggcagac
     3481 ggcgtgggct cgacttgcca tctgccttgg cggcctcccc cgtagggagc ggctctcccg
     3541 tgctgacttc gaggtgtgtt gcggccacgc tcatgcttcg accaaaggag gaacttgcac
     3601 ttcgatccgc cccgcgacga cccgtaccgg gtaaaccggc aagctcacgt tcgggtcatc
     3661 caagcagcgg cccgtacgca gatcgaaact ctgcttgaaa atgggtgacg cgaccttcgg
     3721 gattccccca cggtcaccga cgatgccgcg ggcgattacg aacgctttac tgaaaggatc
     3781 gaagttcgag agcgcgtaca cttggccggt atcgcgggtt cggatgatcg cgatctgctg
     3841 gcccgcgatc atcaccgcca ctcccgtacc cggcaggacg tcactcaagg agcatgcgtt
     3901 gtagtaaggc ataccgtgca tgcgctgcgg ttcgctctcg acgaatgagt tgttcacggc
     3961 gccacctgca cgaccggcaa acgaatctta cccgactcga ccagcggcaa cttctcgtcc
     4021 cagaaagcag gccgatgctg ctcgcgctcc ttgacccaca cgatgctcgg atccgaatcg
     4081 tcggtgttta cgaatgcacg gaacatcttg agcttctcgg gatcttgaac ggtggtcttc
     4141 cactcgcatt ggtaggtatc tacgacgtgc tgcatttggg cttcgagctc ggctccgatt
     4201 cccaagctgt cctcgatgac gacttgcttg acgtattcga tgccgccttc gagcttgttg
     4261 agccaagtcg ccgtgcgctg cagtcgatcg gccgtcttca cgtagaacat caggaatcgg
     4321 tccaggtagc ggattaccgt gtcttcatcg atgtcggccg cgaacaacac cgcgtgttgc
     4381 ggcttcatac cgccgttgcc acacaggtac agattgtatc ccttctccgt cgcgatcacg
     4441 ccgaagtctt taccttgagc ttccgcacac tcgcgtgcgc aaccggaaac ggcacccttc
     4501 agcttgtgtg gtgcgcgcag acccttatag cgattctcga gcttcagggc gagccccacg
     4561 gagtcctgca cgccgtagcg gcaccacgtg ctgccaacgc aggacttcac ggtgcgcaat
     4621 gccttgccat aagcgtgccc cgattcgaag ccggcggcga tgagctcgcg ccagatgctc
     4681 ggcagttgct ccaatcgggc tccgaacagg tcaatgcgct gcgcgccggt gatcttggtg
     4741 taaagaccgt atttcttggc cactctgcca atcgcgatca gctgatcggg agtaatttcg
     4801 ccggccgcaa cgcgtggaac cacggagtaa gtaccatcac gctgaatgtt cgcgaggaag
     4861 cggtcattcg tatcctgcag cccggcgtgc tcggcccgca agatgtagtc gttccaggta
     4921 gaggccaaga tcgaggccac cgccggcttg cagatctcac aaccttgacc ttgcccatgt
     4981 cgcgccaaga gctcgtcgaa cgagcgaatc tcgtgcagac gcaccagctg gtacaagtcc
     5041 tgacggctgt acttgaagtg ctcgcagatg tgattgttga cgcgaatacc cgcgcgcttc
     5101 agctcgcatt tcagaacgtc atctaccaaa gtcgagcagg agccgcagcc ggtgcccgcc
     5161 ttcgagcact tcttgacggc gctgagttcg accagctttt cgtcacgaat caggttgacg
     5221 atgctgcctt tgtccacgtt gtgacaggag cagatgatcg cggcgtcggg cagtgcatcc
     5281 acgccgaggc cctttttccc cccttcgcgc ggcggaacga tcaaatcctc ggggtgttcg
     5341 ggcagtggga gtgagttctg aacgtaagtc gtgagttccg tgtacgcgga tgcatcaccg
     5401 accagaatgc cgccgagcag ctgcttcttg tcttcgctga ggacgagctt cttgtagacg
     5461 cccgcaacat tgtcatacag gctgatgaca cgcgctccgg gggtgcgcgc gaacgcgtcc
     5521 ccaaaactac ccacgtctac gccgagcagc ttgagcttgg tgctcatgtc ggcgtcgacg
     5581 aaattcgcct cttcgcccgc gatccgcgcg gcggcgactt cggccatgcg atagccggga
     5641 gcgaccaagc cataggtttt tcctgagaac gcagcgcact caccgatcgc gaacacccgc
     5701 ggatcgctgg tttggcaacc cacgtcgatt gcaatgccgc cgcgctctcc gatcttcaaa
     5761 ccagcggcgc gcgcgagttc gtcgcgagct cggatgcctg ccgagaacac caccatgtcc
     5821 acgcgcagct cgttgccatc cgaaaaggag agtgcctcgg cgcggccttc tgcattcact
     5881 tcgatgcggg tcgtggcccg gctagtgtgc acggaaaccc cgagctcctc gatgcgacca
     5941 cgtaggatcg caccgcccac gtcgtcgatt tgaagcggca tcaggcggga tgcaaactcc
     6001 acgacgtggg tcttcaaacc aagctgacgc aaggcgttgg cggcctccag acccaggagc
     6061 cctccgccaa tcaccacacc gctcttgcac tcacgagcac agtcttggat cgcacgcaga
     6121 tcctcgatcg tgcggtagac gaagcagcgc gccgacttgt tgcccgggat ttctggaacg
     6181 aaaggccgtg aacccgtagc cagaaccagc cagtcgtagt tcagctgctc gctctggtcg
     6241 cccgagacca cggtcaaagc ccgggccgcc aaatccaact cggatacacg cgcatcgagc
     6301 cgcaggtcga tgccggcgct gcggtactgc tcggcactac ccagcgccaa atcctcggcc
     6361 gacttaccct cgaagtactt cgacaggttg acgcgatcgt aggcaacgcg cggttcttca
     6421 ccgaagcaca ccacctcgta cttggcggtg agcccgcgtc ggatcagctc ctccaagaag
     6481 cgatgaccga ccattccgtt gcccacgacc accagccgcg gtttgctctt tccgttcgtc
     6541 agggagccca tcaatctctc tccacctatt ctcgaccgtt atttcgactt cgcgaccgtt
     6601 ttcgatcttt tcgaccgtgg atgttcggcg attcggctcg cgtcggggag tcgatgtttc
     6661 ggctatcgag tgctcgatct ttcacattcg agcactcgag tcatgattct cagccatcgc
     6721 gaggatgccc tcgagtttcg tgaactcacc gtctgccgac gacgcgattc acatctactc
     6781 ttaggtcgcg aagggtttct tggtttcgtg tgtcaggagt ctcgaatgtt tcgagcttca
     6841 cgcaccggct tcggccaggc tgggaacgcg gcgcaccagg aagctggagc gcaggtagca
     6901 ccaccaagtg agcccaacgc aggtcacgta gaaggcgagg aagtatcgaa ccgcccccga
     6961 ggctccgcct gtggcttgga tcgaggcacc gaaggcacgg gggatgaagt agccgccgag
     7021 cgcgcccgta gccgaagcga tcccgatcac cgccgcagtt tcacgacgcg cggcgacgaa
     7081 cgctgcggct tcggcctctt cgccgcggcc ccgggcgcgc cgcagatgaa gagttcgaaa
     7141 gatcaccggg atcatccgga aggtagagcc gttgccgatt ccggtcgtca tgaacaacat
     7201 caagaacacc accaggaacg cggacaggtt gtgagtctcg agcgaccata gcaatcccaa
     7261 gctcgccacg gccataccta cgaagttcca gaaagtgacg cgagccccgc cgagacgatc
     7321 ggcgatctta ccgcccaggg gacgcgcgac ggaccctacg agcgcaccca ggaacgcgag
     7381 gttggtcgta acttccggga attgagtctt gaggagcagc gggaaagccg cggagtaacc
     7441 cacgaatgag ccgaaggtgc cgatatagag ccaggcgata atccacgtgt ggcgctcttg
     7501 cgtgaccacg acttgatctt tgacgttcga tcgtgcggtg gtcaggttgt tcatgaagaa
     7561 cgtcgcgccg aggacggcca acaggatcag gggcagccac atcaggcccg cattctccag
     7621 gtgaatcttc gaagtgccac cgggcaacaa cgcgtagccg actacgatcg gaaccagcag
     7681 ctgtacggtg ctgactccga tgttgccgcc ggccgcgttg agcccgagcg caaacccctt
     7741 ctcccggtcg ggaaagaaga aggagatgtt ggccatgctc gacgcgaagt tgccgccgcc
     7801 caagcccgcg ctcgccgcgg agagcagcat caaccagaac ggcgtggagg gatcgcgcac
     7861 caacacgatc atcgagagag tcggaatgct cagcagaagc gcgctgacga tagtccaatt
     7921 gcgcccgccg aacttgggca cggccaaggt gtacggaatg cgcgcgagtg caccaaccag
     7981 gccggggacc gcgaccagct ggaagagctg ctcggtcgtg tacggaaagc ctgccttggg
     8041 cagacgagtt gcgacgacgc tccacaccag ccagaccgaa aaaccgacgt tttcagccag
     8101 aatcgaccaa atcaggtttc gacgagcaat gggcttgccg cgctccgccc aaaacttcgg
     8161 gtcttctggg ttccagtcac tgatccagta cttcccatct ctacctaccg aagctttgcc
     8221 taccgaagct gtgtctgacg aagctttgct catcgaagtt ctttctttcc tcgaccgtca
     8281 ttgctgggtc gcgtagctgg cggctcgata cagcgagctc gcgccgcgaa cagcgcctag
     8341 agcaaccgac gtgccatgta tgaaagaagc ggggttgtgt gctgccaatt gacggtttgc
     8401 aacgagcgag aaccgtgcgg aaaagaccta gaaacctagc tccaccgcca accgacgaat
     8461 tcgtcggacg cccctgaatc gagccgacga ttacgtcgtg gcccgacgcg agcccaactg
     8521 aagtcagctg ggcagcctca cgctcgaaag aggcaaaagc agctgtttcc agcgcgcaca
     8581 gcggtgatca gaaaatcccg agaaaactgg gtgaaacgac atttttgtgc gaagggacga
     8641 cagcttgccc ggcacgcacg gtgcggcgat tcatgcgaga aacacagacg aaacctgatc
     8701 acgccgaggg gttggacgcc atgcgatcga gcacgcccag cagaacccgg gcggccgctt
     8761 gaatgtcgtc cgcaccgagg tcgaggtgag tcacgagccg tagcgtggtg ggtgacaacg
     8821 catacagcag caggcccgct cgctcacact cgagcactac tgcgcgcgcg acaggttgcg
     8881 ccagctcggc cacgacgatg ttcgtctccg gatgtttgac ggtcacccct ggggcttctc
     8941 cgatgacctg agcaagccgg agagctgctt cgtgatcgag agccagcctc tcccggtgat
     9001 gttcgaccgc atagagcgca gcagccgcca atacgccgct ctgccgcatt ccgccgccca
     9061 gcatcttcct aaacctgcgc gcgcgatcaa tttcttgctt gcgtcccacc aacgcggagc
     9121 caaccggcgc gcccaagcct ttcgaaaagc agacgctaac ggtgtcgaag ggggcggcca
     9181 gctcctgctc gctcgtgcgg ctcgcaacgg ccgcgttcca caaacgagcg ccgtccaggt
     9241 gaagcgccag gccaagctct ctcgcgcgca ccgcaagccc caggatctcc gtttggggga
     9301 acacgcgccc accgctccga ttgtgcgtat tctcgatcca aacgagcgct gtgcggggca
     9361 cgaagtccga aatggggcgc acggcgcgct ccaagtcctg aaccgtaaaa gctccctccg
     9421 cacctacgct cgtgaactgt acgccgctcc aagctgcacc ggcacccgac tcgtcattca
     9481 cgacgtgggc cgcctccgcc acgacaacct cgtcccctct gcgagtatga acctgcaggg
     9541 caatctggtt gctcatggtt ccagacggga cgaacagcgc ggcctccttg ccgagcagct
     9601 gggccactcg ttcttcgagc cgtctcgtcg ttggatcttc gccgtaaacg tcatccccga
     9661 cttcggcccg
//
LOCUS       DASTKU010001099        12926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_887861, whole genome shotgun sequence.
ACCESSION   DASTKU010001099 DASTKU010000000
VERSION     DASTKU010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 12926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12926
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887861"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1285)
                     /locus_tag="VFQ61_39255"
     CDS             complement(<1..1285)
                     /locus_tag="VFQ61_39255"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HET9960594.1"
                     /translation="MRQHFRGLISGGILLLTACTDWRTPERSALQVDWSTAAEGLAEP
                     VVEIHVRHSGSRSPLPQDMALFEGDITESALHDGDFGLLSRSLHDKRVPLRVDPEPDA
                     SLRVIPQRALSAGFRYTLAARGLGRVALLEVTAASERVLLRRVWPRGAALGSVALFCG
                     GAEPLPAMDFELDGPSGADEFRARARFSPQTEPVVDMEGRVSEACAWLELMGAAPGAS
                     GPAAVAVNWAAPLRFEGYLLEPYNLGWLTVDSVERPSCTASEVELGPACAQVLDDRVI
                     VLAPNVQSAWLLESGERNMSGELRLATEGERVLFRGFMPELRQTLRGWARFATGQQWE
                     FEREVTTARAEPHLQISELLANPLGSEPQSEWIELLNDGAAEQDLEGFSLDDGSGSTV
                     LPHAVVQPGEYVLVVRDDFVGGDPDVPAMPDTQLVH"
     gene            1391..1756
                     /locus_tag="VFQ61_39260"
     CDS             1391..1756
                     /locus_tag="VFQ61_39260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET9960595.1"
                     /translation="MTPEEIQQLRKELTCTARELATTLGVDPKEVSAWEAGELFPTKR
                     YVQAMEGLRARGPSAIVRAPKGKAPKRVGLDRLSDPALWKLFRKLLDHPQLFDDVQAL
                     AAKYPDPPEAKPSESKGTG"
     gene            complement(1796..2962)
                     /locus_tag="VFQ61_39265"
     CDS             complement(1796..2962)
                     /locus_tag="VFQ61_39265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960596.1"
                     /translation="MSLRFGAALRLRRWFSDGTRVLVLALALLAVAARASAGEPAVTP
                     RRTAIALVGASGQSALQARLRAELTQLGWRVQEIAPGAELALAQVARRTHTLAALRVG
                     RGGEGIELWVAPEADSEQARSEWIDAAGGRGDLAVLRAVEVLRVRFVEFGLEPERDET
                     SEADAPQPAALDPKPAPEPPPAETKVERRSVASLTPASPGSSQPLTEWPRFWLGLGGG
                     ALQSYSDAFVPQPLASLSARYALAKPRPGLTIAALDARVLFPVQARTLEDERGSVAVR
                     TFQGALGVEARWITGRFTSGVGVGAGLSVLNLTASAKPGYAARHVLVFSPFPLLRAAT
                     ELELTRGLRLRLDLSASRAVPRAAIRFDESEISSFGRPLMGAAVLELLWAPSAN"
     gene            complement(3057..4349)
                     /locus_tag="VFQ61_39270"
     CDS             complement(3057..4349)
                     /locus_tag="VFQ61_39270"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HET9960597.1"
                     /translation="MTEPELVERELLQRLGRRVAATQDAWLTRAVPLDAARDGFLLRR
                     GEKRKASAHRIWWVAAAAALALIPSGYWARQAWFQPGPVAAFVHDKPVLQDSWIDASS
                     QQPVPIHFSDGSDMVLAPGARLRVAELHPDGARVAVETGAVDVHVVHRTRTDYRLNLG
                     PFLVRVTGTRFAVSFSPEQDVLRLKMQEGSVTVSGCSLGEARPVRAGESLVASCKDAR
                     FEISRGAAGSTRDAQVPAEAGPLGAVVPEGSANVGSSAGSEELNGPPSNAANGPPVRS
                     GPSWQALAKKGKYAAALGLLEQLGFEAQCERVGSDELTLMADVARFGKRSDRAVLALQ
                     ALRRRFSGSGAASVAAFSLARIHFDQRAAYDEAARWFRTYLKEQPGGSLAREAQGRLM
                     EALYRGGDRAGAERAAEAYLARNPEGPHARLARSLLGR"
     gene            complement(4346..5002)
                     /locus_tag="VFQ61_39275"
     CDS             complement(4346..5002)
                     /locus_tag="VFQ61_39275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9960598.1"
                     /translation="MERLPLSQRPLARVDAAPRSGAVIALHSDSDAALVRALREREPH
                     AGTALFDRYGMHVRRVLARVLGPDPELGDLLQDVFVSALSSIHTLEDPSALRAWLTRM
                     AVFTARGRIRRRSRWRFLKFVADDELPEPLAPAQDAEGSQALSAVYRVLKCLPADQRI
                     VFALRVFDGMELNAVATACGVSLSTAKRRLARAQTRFMELSRREPVLADWVAHAAEAT
                     "
     gene            5232..5909
                     /locus_tag="VFQ61_39280"
     CDS             5232..5909
                     /locus_tag="VFQ61_39280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960599.1"
                     /translation="MLLVAVGCGDILVKPVGKDSSEVVGLCAPCESSVTCGDENDYCV
                     LRSSVAQAFCSRDCEDEGECPVGYTCVATPGAMARQCIPDAEQCLPPQNSPSPDELRS
                     KLVERMRTWRSERGLSSFEASACLNLLAQLSIDDRIAGGKPLGKLEPSCRSDSSCECG
                     WKEERQAQLGGYGLDLDSALDASLTTDHVATTSPAAVLDAKDFKQLGIGARKSGDELF
                     VSFLLAQ"
     gene            complement(5926..7854)
                     /gene="scpB"
                     /locus_tag="VFQ61_39285"
     CDS             complement(5926..7854)
                     /gene="scpB"
                     /locus_tag="VFQ61_39285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET9960600.1"
                     /translation="MSKRKPPQNPAKSGASKVAPSKAAPRGTSRKARVEPALSNEKRP
                     GKKSPPPAKPEPRKPAKGTAGGTKSGERERESSSDQDESKVARRAAKTLGKPEPSEAA
                     RVARGEPERAEGGGKRGRTLPSVAEPPSEEADAAADEVELELSAVFGDDQEFPPDLQP
                     VASEAAPETLPPSIATSGPDSGDAVTLRGAGPAAFQSPDGLGEPGPQKRMGLAEAAPE
                     ETGFAPSTQQDPVAQRMPEAEALARLSERDRSERDPAEGHGAERDELGSAVASDDLEV
                     EEAVESETVTDVSELLNAPGFEERHAAQTPEDTEQYLRGLVEAILFVSDKPLELKDLA
                     RAVKLDRKRTQELVSDLMANTRQRGIRIEEVAGGYVFRTNPAYSSEVRAFLQQRPVRL
                     SRPQVETLAIVAYRQPITRPEIDDIRGVDSGPVLKGLLERDLIRIIGKKDEPGRPMLY
                     GTTPTFLESFSLRSLRDLPTLREYTELTEESRQAFETQLGETAPQGSVHQETGASGGA
                     DDRDDGAPPMSRVTADSTDARRPSQEDEGGGATEPNDSVGPSSEAPEDSGDEENLDDD
                     ESDDDDESDDDDESDDDDESDDDDESDDDDESDDDDESDDDDESDDDDESDDDDESDD
                     DDDESDDDEDDEESDDDE"
     gene            complement(7854..9242)
                     /locus_tag="VFQ61_39290"
     CDS             complement(7854..9242)
                     /locus_tag="VFQ61_39290"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET9960601.1"
                     /translation="MSEPPSAPPAPEAASSAADAPENPASAAQDPAAMGSAESSEPGS
                     ASETPDAAANSSEKEAREEGNRTARSKSRSERLSKQAASSEAASAKPSADSVEGYRVA
                     LPAFEGPLDLLLHLIEEHELDIRDIPIAFIAEKYVQYITLMEELNIDIASEYLVMAAT
                     LAYIKSRSLLPTPPAEEIDEEEVELDPRQELIRRLLEYQKYKHVAERLAEKDLLGRDV
                     FPRGMKAPDVEGPAPLAGMSLFKLLDAFQSVLSRATLKVDHQIDFERFSISDRINEIS
                     DLLRKNPRLSFDELFEGQRTRAELIVTFLALLEMAKLRLIRLFQDGPLEVIYIELAVS
                     DVDKNDASDELSRPSTAGLEPDSPEDFEASADPVPVEDEEPLEEDDLFGDGPGAQRFD
                     ADDWEDGESEFNIDPELDDSEDLERDASPPASEPEPEGSSGFGGLSEDDAEPNADDAP
                     NDEDDAPEREPR"
     gene            9447..10796
                     /locus_tag="VFQ61_39295"
     CDS             9447..10796
                     /locus_tag="VFQ61_39295"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9960602.1"
                     /translation="MAQCVRPDAEYPLGLVIDQQFRIDACLGQGSMARVYRAHQLQVG
                     RDVALKIMRRELLGQPEVIARFREEAGLVARLSHPHVVVVYGVGQMPPRGPLPGDSGS
                     TTADAREVFGTNSAVGEPYVVLELLQGRSLAQALDDAGGVLPLTRTLHVILALADAVG
                     EAHARGIVHRDVKPDNVMLVERGSDHDFVKLLDFGLAKLLTEEREIRTRAGAILGTPH
                     YVSPEGAQGQPVTPAADCYSLAALLFRCLAGRLPFEAPSAVTVLAQKASQPAPDLRSF
                     PRAATVPSSIADLIMQNLARKPEQRAPNARAFGQGLVEAARLAGIEERDFGLSQTLVG
                     ARAIQLQSLTQSQESSPSSAASARSSPSSVTISKSIERAASTSESSASNQLSTASSQA
                     QSPDSRAPVPNRSALWLVLACFIVGVLAAAAWGAWGLDPSKSAPPDAPPPTRAGSSR"
     gene            10793..11554
                     /locus_tag="VFQ61_39300"
     CDS             10793..11554
                     /locus_tag="VFQ61_39300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960603.1"
                     /translation="MRDARVHATLGLLLSGFWLLRAVSAGAQEDGARSVGGSSVNFDI
                     EPAVGFAALRRPTGILEAGFGWLTLPGADICIVRGSDCAKGDTSFEVDAWQLYRPSKR
                     VAFGAGVLLGLVPTTVAETNAPRDHSRRYFTAEGTIRYYPYVGKNVEWWVGLTGGLVV
                     VSDTFKVRGENDSARALQGPSGVTIRTEGGSIGLAGGPVVALSSYWALGATLRYGQWF
                     LPTQPAVDSLGSEASLRGRNSIFSLGVNIAFRLPL"
     gene            11709..12551
                     /locus_tag="VFQ61_39305"
     CDS             11709..12551
                     /locus_tag="VFQ61_39305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HET9960604.1"
                     /translation="MNLDLSTVGQRTEAFPFEYDFHQTILYALGVGARKEELDFLYEG
                     RGPKVLPSFAVVPSYAATAVLFERTRSDLTRLLHQAQTVRLHRPLPAAAKLETVGIVK
                     GIYDMKRFAQIVLVTESTCRGELLFETEWTLIVRDAGNFGGPRPPKSEIFRPESGQEP
                     AFKTEASTSPEQALLYRLCGDLNPLHADPEFAQAAGFNEGPILHGLCTFGVATRAIVQ
                     HLGGDVGRVRSLSAQFRKPVWPGDTIRTEGYALANGRLAFEAFAADRPDPVLTQGLVE
                     LNPV"
     gene            12564..>12926
                     /locus_tag="VFQ61_39310"
     CDS             12564..>12926
                     /locus_tag="VFQ61_39310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960605.1"
                     /translation="MSDEHSKKVSSTPPPPTDAVDQSWSNTPEPGRVDLGKPVTSTAV
                     APGVSAQDIRRATRHAEEEELDDDDEDEDEDEDEDSDEDEDEGEHEEAPARALSGTNR
                     SGASNDWLPDWAPYAVLGA"
BASE COUNT         2257 a         4321 c         4032 g         2316 t
ORIGIN      
        1 cgtggacgag ttgcgtgtct ggcatcgcgg ggacgtccgg atcgccgcca acgaagtcgt
       61 cgcgcacgac cagcacgtac tctccgggtt gcacgacagc gtggggcaag accgtgctgc
      121 cgctaccatc gtccagcgaa aatccctcga ggtcttgctc cgccgccccg tcgttcaaca
      181 gctcgatcca ctcggactga ggctcactcc cgagcgggtt ggccagcaac tccgagatct
      241 gcagatgggg ttccgcgcga gccgtcgtga cttcgcgctc gaattcccat tgctgcccgg
      301 ttgcgaagcg agcccagcca cgcaaggtct gccggagctc tggcatgaat ccgcgaaaca
      361 ggaccctttc accctcggtg gcgagccgca gttctccgct catgttcctc tcaccactct
      421 cgagcagcca ggcgctctgt acgtttggcg cgagcacgat cacgcggtcg tccaagacct
      481 gagcacaagc gggcccaagc tcgacctccg acgccgtgca cgatggtcgt tcaacggaat
      541 ctaccgttag ccagcccaag ttatagggct ccaacaagta tccctcgaac cgcaacggtg
      601 cggcccagtt gacggccacc gccgcaggcc cgctcgcccc aggcgctgcg cccatgagtt
      661 cgagccaagc gcaagcctct gagaccctac cttccatgtc gaccaccggc tcggtttggg
      721 ggctgaaccg agcacgagcc ctgaactcgt ccgcgccgga cgggccatcg agctcgaagt
      781 ccatcgcggg caagggctcc gctccgccac aaaacagagc gaccgacccg agcgcagcgc
      841 cacgaggcca tacacgtctg agcaaaacac gttcgcttgc cgcggtgact tccagcaacg
      901 cgacgcgtcc caagccgcgc gccgcgagag tgtatcgaaa gccagcggag agcgcccgct
      961 gcgggatcac gcgcaaactc gcgtccggct cgggatcgac ccgtaacggt acgcgcttat
     1021 cgtgcagcga tcgcgacagt aggccgaagt cgccgtcgtg cagcgccgac tccgtaatgt
     1081 ctccttcgaa tagggccata tcttgaggca gaggcgacct gcttcccgag tgccggacgt
     1141 gaatttcgac gaccggctcg gccaggccct ctgcggccgt tgaccagtcg acttgaagtg
     1201 cgctccgctc cggtgttcgc caatcggtgc acgcggtgag caggagaatg ccgcccgaga
     1261 tgaggcccct gaaatgctgc cgcatatgca ggtatcgacc cgccgaaggc tcgggttgcg
     1321 cgaatctgac ccgaagtttc gaatcggtac gaagcttcga atctaggtcg cttcggggta
     1381 agctcacccc gtgacgcccg aagagatcca gcaactccgc aaagaattga cctgcaccgc
     1441 gcgcgaactg gctacgactc tgggcgtgga tcccaaggag gtttccgcct gggaggccgg
     1501 cgagctcttc ccgaccaagc gttacgttca ggccatggag gggctcagag ctcgtggtcc
     1561 atccgcgatc gtgcgtgcac ccaaaggcaa agccccaaaa cgtgtggggt tggatcggct
     1621 ttcggacccc gcgttgtgga agctctttcg caagttgctc gaccacccgc agttattcga
     1681 cgacgtgcag gctctggccg ccaagtaccc cgaccctccg gaggcgaaac cgagcgagag
     1741 caaagggacc ggctaagcat tcgaccggaa gcccgaacct gctggaatgc tgcgttcagt
     1801 tcgcgctcgg ggcccaaagc agctcgagta ctgccgcgcc catcagcggt cgaccgaagc
     1861 tcgagatctc gctctcgtcg aagcggatgg ccgctcgggg cacagcgcgg ctggcgctca
     1921 aatccagtcg gaggcgcaac ccgcgcgtga gctccaattc agtggccgct cgcaagagcg
     1981 gaaacggact gaacaccaat acgtggcgcg cggcataacc tggcttggcg ctcgccgtga
     2041 gattcaagac cgagaggccc gcacccacgc cgactccgct cgtgaaccgc cccgtgatcc
     2101 agcgcgcctc gactccgagt gcaccttgaa aggtacgcac ggccacggag ccgcgctcat
     2161 cttctagagt gcgcgcctga acaggaaaca agacgcgcgc gtccagcgcg gcaatcgtca
     2221 gcccggggcg aggcttcgcc aaagcatagc gggcactgag gctggccaat ggctggggga
     2281 cgaaggcgtc gctgtaggat tgcaacgcgc cgcctcccaa acctagccaa aagcgcggcc
     2341 actccgttag cggctgactc gagcccggag atgctggggt taacgacgcg acagaccggc
     2401 gttcaacttt ggtctcggct ggcggaggct cgggcgcagg ttttgggtcg agcgcggcgg
     2461 gttgcggagc atcggcttcg ctggtttcgt cgcgttccgg ttcgagcccg aattccacga
     2521 acctcacccg cagcacctcg accgctcgta gcaccgcgag atccccacgc ccgcccgcag
     2581 cgtcgatcca ttcgctgcgg gcctgctcac tgtccgcctc gggcgcgacc caaagctcta
     2641 tcccctcacc cccacggccc acgcgtaaag ctgccagcgt atgcgtccgc cgcgccacct
     2701 gcgcgagcgc gagctccgca ccaggcgcga tctcctgcac gcgccagccc aactgagtca
     2761 gctccgcgcg caagcgtgcc tgcaaagcgg attgacccga ggcacccacg agcgcaatcg
     2821 cagtgcggcg cggagtgacc gctggttctc ccgcggaagc ccgcgcggcg acagccagca
     2881 aggcgagagc caaaacgagc acccgagtgc cgtcgctgaa ccaccttcgc aaacggagcg
     2941 ccgcaccgaa tcgcaggctc atgccgccgc cgcagtgttc atgagcgcgc cctgctgtct
     3001 tctcgcgccc tgctgtcttc tcgcgccctg ctgtcgcggt tgggcggtgc gtcgactcag
     3061 cgtcccaaca gtgagcgcgc aagacgcgcg tgcggtccct cgggattgcg cgccagatag
     3121 gcctccgctg cacgctctgc gccggcccga tctccgcccc gatacagggc ttccatcaga
     3181 cgcccttggg cctcgcgggc gagcgagccg ccgggttgct ccttcagata ggtccgaaac
     3241 catcgcgctg cttcatcata cgcagcgcgt tgatcgaagt gaatgcgggc caagctgaaa
     3301 gcggcgacac tggcagcccc gctgcccgaa aaacggcggc gtagcgcctg cagcgccaag
     3361 accgcgcgat ccgaacgctt gccgaagcga gccacatcgg ccatcagggt tagctcgtcg
     3421 ctgcccacgc gctcgcactg agcctcgaag ccgagctgtt cgagtaagcc cagcgcggcc
     3481 gcgtacttgc ccttctttgc cagtgcctgc caagacggtc ccgaacgtac aggtggcccg
     3541 ttcgcggcat tcgagggggg cccattgagc tcctcgctac cagcgctact gccaacgttg
     3601 gcggatccct ccgggacaac cgcacccaag ggacccgctt cggcgggcac ctgagcatct
     3661 cgagtggagc ccgcagcgcc gcgcgagatc tcgaagcgcg catccttaca cgaggcaacc
     3721 agggactcgc cggcccgcac tggccgcgcc tcgcccagcg agcagcccga aacggtaacg
     3781 ctgccttcct gcatcttcag acgcagcacg tcctgctcgg gtgagaagct cacggcgaag
     3841 cgtgttcccg taacgcgaac gaggaaaggg cccaagttga ggcgatagtc cgtgcgggtg
     3901 cgatgtacga cgtgcacatc caccgcgcca gtctccacgg ctacccgagc accgtcggga
     3961 tgcagctccg caacccgcaa ccgcgcgccg ggagcgagca ccatgtccga accgtccgag
     4021 aaatggattg gcaccggctg ttgggagctg gcgtcaatcc atgaatcttg cagtaccggc
     4081 ttgtcgtgca cgaacgcggc gactggcccc ggttggaacc aagcctgccg agcccaataa
     4141 ccagagggaa tcaaggccaa cgccgccgcc gcggcgaccc accagattcg gtgtgccgag
     4201 gccttacgct tctcgccccg ccgcagcaaa aacccgtccc gagcggcgtc caagggtacg
     4261 gcccgcgtca accacgcgtc ctgggttgcc gccacccggc gcccgaggcg ttgcagtaat
     4321 tcgcgctcca cgagctctgg ctccgtcatg ttgcctccgc agcgtgggcg acccaatctg
     4381 cgagaactgg ctctcggcgc gaaagctcca taaaccgcgt ttgcgcgcgc gcgagacgac
     4441 gcttcgcggt ggacaacgac acgccacagg ctgtggcaac ggcgttcaac tccatgccat
     4501 cgaacacccg caacgcgaac acgatgcgtt ggtcggcagg caaacacttg agcacgcgat
     4561 acacggctga tagcgcctgg ctgccctctg catcttgagc gggcgccagc ggctcaggca
     4621 gctcgtcatc ggccacgaac ttcagaaagc gccacctcga tcggcgccgg attctccccc
     4681 gagcggtgaa cacggccatg cgcgtcaacc aggcgcgcag cgcggaaggg tcctccaagg
     4741 tgtggatcga agacaacgcc gatacgaaca cgtcctgaag cagatccccg agctctggat
     4801 ccggtccaag aactcgcgcc agcacgcgac gaacatgcat cccgtagcga tcgaacaggg
     4861 ccgtcccggc gtgaggctcg cgttcgcgca gcgcccgaac caaggccgca tcggaatccg
     4921 agtgcagcgc gatcaccgcc cccgaccgag gggccgcatc tactctggct aaggggcgct
     4981 gactcaaggg aagacgctcc acactgtctg tgtcctaggg ccgctcggaa ggttcacctt
     5041 gtggatggat gcccggctga gcgcccgagc gcgcgactat tctgcgggaa gaaaaacgtc
     5101 ggcctgatgc ccttggagcg ctcgcgcaat cccgccgaag agtccaaggc caccatgcct
     5161 ataatcgcga cgtgacgcac gtgcagaaat ccggcccacg taccaggaag ctccgcttta
     5221 tcggtttggt catgctcctc gtcgcggtcg gttgtggcga catcttggtc aaaccggtcg
     5281 gaaaggactc gagcgaggtc gtgggcttgt gtgcgccatg cgagagcagc gtgacgtgcg
     5341 gggacgagaa tgactactgc gtcttacgca gctctgtcgc ccaggccttc tgcagtcgtg
     5401 actgtgagga cgagggcgaa tgcccagtag gttacacgtg cgtcgcgact ccaggagcga
     5461 tggcgcggca atgcattcca gacgctgagc aatgcctgcc ccctcagaac tcgccgtcgc
     5521 ccgacgagct ccgctcgaag ctcgtggagc ggatgcgcac gtggcgctcg gagcgtggct
     5581 tatcgtcgtt cgaagcttct gcttgtctga accttttggc tcagctcagc atcgatgatc
     5641 gaatcgccgg gggtaagcct ctcggtaagc tcgagccgag ttgtcgctcc gattcgagct
     5701 gcgagtgcgg ctggaaggaa gagcgccagg cgcaacttgg aggttacggt ctcgatctcg
     5761 actcggcctt ggacgccagc ctgacgacgg accacgtggc gaccacgagc ccggccgcgg
     5821 tgctcgacgc gaaggatttc aaacagcttg ggatcggagc gagaaagtca ggggatgagt
     5881 tgttcgtgag cttcttgctc gcgcaatgac ggtaaacgac gcgggctact cgtcgtcatc
     5941 cgactcctcg tcgtcctcgt cgtcatccga ttcatcgtcg tcgtcgtccg attcatcatc
     6001 gtcgtccgat tcatcatcgt cgtccgattc atcatcgtcg tccgattcat catcgtcgtc
     6061 cgattcatcg tcgtcgtccg attcatcatc gtcgtccgat tcatcatcgt cgtccgattc
     6121 atcatcgtcg tccgattcat cgtcgtcgtc cgattcatca tcgtcgaggt tttcttcgtc
     6181 gcccgagtcc tcgggggctt ccgaggaggg gccgactgag tcgttgggct cggtggcccc
     6241 cccaccctcg tcttcttgcg aaggccgccg cgcgtccgtg gaatcggcgg tcacgcggga
     6301 catgggcggc gccccatcgt ctcgatcgtc agcgccaccg gaggccccag tttcttgatg
     6361 cacgcttccc tgcggcgccg tctctccaag ctgcgtctcg aaggcttgtc gagattcctc
     6421 ggtcagctcc gtatactcgc gcagagtcgg cagatccctc aagctacgca agctgaagga
     6481 ctcgaggaag gtcggtgtag ttccatacag catcgggcga cccggctcgt cttttttgcc
     6541 gatgatccgg atcaagtctc gctccagcaa tcccttcaac acaggcccgg agtccacccc
     6601 gcggatgtca tcgatctctg ggcgagtgat cggctgccga taagccacga tggccagcgt
     6661 ctcgacttgc ggccgcgaca gacggacggg gcgttgctgc aggaacgcgc gcacctcgct
     6721 cgagtaggca ggattggttc gaaacacgta cccacccgcc acctcttcga tacgaatgcc
     6781 gcgttgacgc gtattcgcca tcaggtcgct cacgagctcc tgagtgcgct tgcgatcgag
     6841 cttcaccgct cgagccaggt ccttcaattc gagcggtttg tccgagacga acaggatcgc
     6901 ctcgaccaac ccccgtaggt attgctccgt gtcttccggg gtctgtgccg catgacgctc
     6961 ctcgaagccg ggtgcattga gcagctcaga gacgtcggtg accgtctccg actcgacggc
     7021 ttcctccacc tcgagatcat ccgaggcgac cgcggagcct agctcatcac gttcagcccc
     7081 gtgtccctcc gccgggtccc gttcgctccg gtcccgttcg ctcagacgtg ccaatgcctc
     7141 cgcttctggc attcgctgcg cgaccgggtc ttgctgagta gacggagcaa aaccggtctc
     7201 ctccggggcc gcttccgcta ggcccatgcg cttctgcggg cctggctcgc cgaggccatc
     7261 tggggattgg aatgcggcgg gcccggctcc gcgtagcgtc actgcgtctc cggagtctgg
     7321 cccggaagtg gcgatcgacg ggggcaatgt ttcgggagcg gcttcgcttg cgacgggctg
     7381 taaatcgggc gggaactctt ggtcgtcccc gaacacggcg ctcaactcga gttcgacctc
     7441 gtcggcagca gcgtcggctt cttccgaagg gggctccgcg acactcggta gcgttcggcc
     7501 tcgtttgcca ccaccttccg cccgctcggg ctcgcctcgc gctacgcgag cggcttcgct
     7561 cggctccggc ttcccgagcg tcttcgccgc cctgcgcgcg accttcgact cgtcttggtc
     7621 ggagctgctc tcgcgttcac gctcgcccga cttcgtgcca ccggccgtac cctttgcggg
     7681 cttgcggggc tccggcttgg caggtggcgg tgacttcttt cccggccttt tctcgtttga
     7741 cagcgctggt tccacccgcg ctttgcggga ggtgccgcgc ggggcggctt tcgacggcgc
     7801 gaccttcgac gcacctgatt ttgccggatt ttggggaggt tttcgtttgg acattatcga
     7861 ggctcccgct cgggggcgtc atcttcgtcg ttcggagcat cgtcggcgtt aggctctgcg
     7921 tcgtcttcac tcaggccgcc aaaaccggat gagccttctg gctcgggctc cgacgctggc
     7981 ggtgacgcat cccgttcgag atcctccgag tcatccagct cgggatcgat gttgaactcg
     8041 gactcgccgt cttcccaatc atctgcgtcg aagcgctgcg caccgggccc gtcgccgaag
     8101 agatcgtcct cctcgagcgg ctcctcgtcc tcgaccggaa cggggtcggc ggaagcttcg
     8161 aaatcttcgg gggagtctgg ctctagcccc gctgtcgacg gacggctcag ctcgtctgag
     8221 gcgtcattct tgtcgacatc gctcacggca agctcgatgt agatcacctc gagggggcca
     8281 tcctgaaaca gccggatcag ccgcagcttc gccatctcga gcagcgcgag gaaggtgacg
     8341 atcaactcgg cgcgcgtgcg ttgtccttcg aacagttcgt caaatgacag gcgcgggttt
     8401 ttgcgcagta ggtccgagat ctcattgatg cgatcactga tcgagaagcg ctcgaaatcg
     8461 atctgatggt cgaccttcaa cgtggcgcgc gagagcacgg actggaaagc atcgagcaac
     8521 ttgaacaggc tcatgccggc gagcggcgcg ggcccctcca catccggcgc cttcattccg
     8581 cgcgggaaca cgtctcgacc cagcaagtcc ttctccgcga gccgctcggc gacgtgcttg
     8641 tacttttgat actcgagcag gcgccggatc agctcctgcc gcggatcgag ctcgacctcc
     8701 tcttcgtcga tctcttccgc aggaggggtc ggcaacaggc tgcgcgactt gatgtatgcg
     8761 agggtggccg ccatcaccag atactcgctg gcgatgtcga tgttcagctc ctccatcagc
     8821 gtgatgtact gaacgtattt ttccgcgatg aacgcgatcg ggatgtcgcg aatatcgagc
     8881 tcgtgctctt cgatcaggtg caacagcagg tcgagcgggc cctcgaaggc cggcagcgcg
     8941 acgcgataac cctcaacgct gtccgcactt ggcttggcgc tggctgcctc ggacgatgcc
     9001 gcttgcttgg agagtcgctc cgatcgcgac ttgctgcgcg cggttcgatt tccctcttcg
     9061 cgggcctcct tttccgacga gtttgccgcc gcatccggcg tctcggacgc agagccgggc
     9121 tccgaagatt cagccgaacc catcgcagcc gggtcttgcg ccgctgacgc cggattttct
     9181 ggggcatccg cggcagacga agccgcctca ggggcgggtg gcgcggaagg gggttcgctc
     9241 atcaggaggg gcccgcagcg tatctggggg gcagggattc gcaaacggat ttcgctggca
     9301 ctgccaggcg ctgacacagc cgtctgaccg ctcgaaccgg cgtggacgcc ccgttacata
     9361 agaacctatt gcagcgctct gcacgagcga ctgcagatcc ccaaatctga gacgaactct
     9421 aggtgtacag atgctaggtt ccagccatgg cgcaatgcgt ccggcccgac gcagagtacc
     9481 cgcttggcct ggtgattgat cagcagttcc gaatcgacgc ttgcttgggt caagggtcga
     9541 tggcgcgggt gtaccgcgct caccaactcc aagtcggacg cgacgtcgcg ctcaagatca
     9601 tgcggcgcga gctgctcggg cagcccgagg tcatcgcgcg ttttcgcgaa gaggcggggc
     9661 tcgttgcgcg gctctcccat ccgcatgtcg tggtcgttta tggggtgggt caaatgccgc
     9721 cgcgcgggcc actccccgga gattcaggtt ccacgactgc tgacgctcga gaagtctttg
     9781 ggacgaactc agcggtgggt gagccgtacg tggtgctcga gcttttgcaa ggtcgctcgc
     9841 tcgcgcaggc cctggacgat gcgggcggag tgctgccact cacgcgcacg ctgcacgtaa
     9901 ttttggcgct cgccgacgcg gtcggagagg cgcatgctcg gggcatcgtg cacagggatg
     9961 ttaagccaga caacgtcatg ctcgtcgaac gcggcagcga tcacgatttc gtgaagctgc
    10021 tcgactttgg cctggcgaag ctgctcacgg aggagcgcga gattcgcacg cgcgcgggag
    10081 ccatcctcgg aactccgcac tacgtgtcgc ccgagggggc gcaggggcag cccgtcactc
    10141 ccgcggccga ttgctactca ctggcggccc tgctcttccg ctgtctcgcg gggcgactgc
    10201 ctttcgaagc tccgagcgca gtcaccgtat tggctcagaa ggcgagtcag cccgcgcccg
    10261 acctacgttc gtttcctcgc gccgccacgg tcccgagtag catcgccgac ttgatcatgc
    10321 agaacctggc gcgcaaaccc gagcaacgcg cgccgaacgc gcgcgcgttc ggacaaggct
    10381 tggtcgaagc tgcgcgtctc gctggcatcg aagagcgaga cttcgggctg agtcagacgt
    10441 tggtcggcgc ccgcgccatt cagcttcaaa gtcttacgca gtcacaggag agctcgccga
    10501 gctcggcggc aagcgcgcgg tctagtccat cgagcgtcac tatctcgaag tccatcgaaa
    10561 gagcggcctc gaccagtgag tcatcggcct cgaaccagct atccacggcc tcgagccaag
    10621 cgcagtcccc tgactcacgc gcccccgtcc cgaaccgttc cgcgctgtgg ctggtgctcg
    10681 cctgcttcat cgtaggcgtc ctcgccgccg cagcctgggg cgcttggggt ctcgatccga
    10741 gcaagtctgc gccgccggac gcgcccccac cgacccgcgc cgggagctcg cgatgagaga
    10801 cgcccgagtg cacgccacac tgggcctctt gctcagcggc ttctggctac ttcgcgctgt
    10861 cagcgccggc gcccaagagg atggcgcgcg ctcggtcgga ggctcgtcgg tcaacttcga
    10921 tatcgagccg gcagtgggct tcgcggcact caggcggcca accggaattc tggaagctgg
    10981 ctttggctgg ctgactttgc cgggagctga tatctgcatt gtacgcggca gtgactgcgc
    11041 gaagggcgat accagcttcg aggtcgacgc ctggcaactc tatcgcccca gcaagcgcgt
    11101 cgcgttcggc gcgggcgtac tgctcggcct ggtccccacc acggtggccg agaccaatgc
    11161 gccccgcgat cacagccgcc gctacttcac ggccgaaggc acgattcgct actaccccta
    11221 cgtgggcaag aacgtcgaat ggtgggtggg gctcaccggg ggcctggtcg tggtgagcga
    11281 taccttcaaa gtgcgcggtg aaaatgactc cgcgcgcgct cttcagggcc cctcgggggt
    11341 gacgatccgc actgagggcg gcagcatcgg cctggcgggt gggcccgtgg tcgcgttgag
    11401 ttcgtattgg gcgctggggg caacgctgcg ttacggtcag tggtttttgc cgacgcaacc
    11461 cgctgtggac tcgctcggga gcgaagcgtc tttacgcggc cgcaactcga tcttctcgct
    11521 cggtgtgaac atcgcgttcc gtttgccgct ctgagtcgcg ccagcctcga accctccggg
    11581 ccgcgcgacc aacgcccggc gcgcacccgg ctcggtcatt tcgcgccttt ccagggcgag
    11641 attcggagag cccacattga ctcaagtccg tttcgcgcga taacggtggc cctcctccgg
    11701 gtgacacgat gaatttggat ctctctacgg tcgggcaacg aacggaagca ttcccgttcg
    11761 agtacgactt tcatcaaacc atcttgtacg cactcggagt cggggcgcgg aaagaggagc
    11821 tcgactttct gtacgaaggg cgcgggccca aggtgttgcc gtcgttcgcg gtcgtgccgt
    11881 cttatgccgc aaccgcggta ttgttcgaga gaacacgcag cgatctcacc cgcctcttgc
    11941 atcaggcgca aacggtacgc ctgcaccgcc ccttacccgc ggcggccaag ctcgagaccg
    12001 tcgggatcgt gaagggcatc tacgacatga agcgcttcgc gcagatcgtg ctcgttaccg
    12061 agagcacttg ccgcggcgag ctgttgttcg agaccgagtg gaccctgatc gtgcgcgatg
    12121 ccggaaattt cggcgggcct cgtccaccga agagcgagat attccggccc gagtctggcc
    12181 aagaacctgc attcaaaacc gaagcatcca ccagcccgga gcaagcgctc ctgtaccgtt
    12241 tgtgcgggga tctgaacccc cttcacgcgg acccggagtt tgcgcaagcc gcgggcttca
    12301 acgaggggcc gattctgcac ggcctctgta cttttggcgt tgccacgcgt gccatcgtcc
    12361 agcacctcgg tggagacgtc gggcgggtgc gcagcctgag cgcgcagttc cgcaaacctg
    12421 tttggcctgg ggacacgatc cgcaccgaag gttacgcgct cgcgaacggt cgcctcgcgt
    12481 tcgaagcctt cgcggcagac cgccccgatc ccgttctcac ccagggcttg gtcgagctca
    12541 atccagtatg aggagcactc gcgatgtctg acgaacactc gaaaaaagtt tcctccactc
    12601 cgcctccccc gacggacgcg gtcgaccaaa gctggagcaa cacgcccgag ccaggtcgcg
    12661 tcgatctcgg taaacccgtg acctcgacgg cagtagcacc cggcgtgagc gcgcaggata
    12721 tccgtcgcgc cacccgtcac gccgaagagg aagagctcga cgacgacgac gaggacgagg
    12781 acgaggacga ggacgaagac agcgacgagg atgaggacga aggcgagcac gaggaggcgc
    12841 cggcccgtgc tttgtctggc accaatcgca gcggggcctc gaatgattgg ctgccagact
    12901 gggcaccgta cgccgtactc ggcgca
//
LOCUS       DASTKU010001100         9485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1560114, whole genome shotgun sequence.
ACCESSION   DASTKU010001100 DASTKU010000000
VERSION     DASTKU010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560114"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /gene="pgk"
                     /locus_tag="VFQ61_39315"
     CDS             <1..422
                     /gene="pgk"
                     /locus_tag="VFQ61_39315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET9960606.1"
                     /translation="FPIDFVITDKFDFKARTVGATRVVGRDKIEPGTEALDIGPESIK
                     AFEKILLGAKTIIWNGPVGVFEIDATAKGTIAVAEILAKATAAGATTIIGGGDSASAV
                     KKAGVASKVSHVSTGGGASLEYLEGKVLPGVAALTNA"
     gene            635..2203
                     /locus_tag="VFQ61_39320"
     CDS             635..2203
                     /locus_tag="VFQ61_39320"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HET9960607.1"
                     /translation="MKIGIPKEIRPAERRAAATPDTVQRLRKLGFDVEVETGAGAGAS
                     FDDAAYMAAGATIAADAKSLWSNADLVLKVQPPELNTSDGVHEAELLREGGLLISFLW
                     PAKNAELIERLRARKGTVLAMDQVPRITRAQKIDALSSMANIAGYRAVIEAASFYGRF
                     FTGQMTAAGKVPPAKVLVIGAGVAGLAAIGAARGLGAIVRAFDTRSEVRDQVQSMGAE
                     FLEVEIQEEGGGGGGYAKQMSPEFIAAEMALFLRQAKDVDIIITTALIPGKPAPVLIT
                     EEMVRAMRPGSVIVDLAAENGGNCAFTRPGQVVVESGVTVVGYVDLPSRLAPLASQLY
                     GTNLVHLLTDMGGNANFRVDLEDQVVRGSIVLKDGELLWPPPKAPPPAAPVSKKPEAK
                     IAAHGPGHAKPTPAPLPKAFVAAAWLVGAIGIGALGLLAPKEFLSHLTVFVLACFVGW
                     QVIWNVTPALHTPLMSVTNAISGIIIVGGMLQLTFDFSSPVTWVGAFSVLLAAINVVG
                     GFLVTQRMLRMFHR"
     gene            2211..3650
                     /locus_tag="VFQ61_39325"
     CDS             2211..3650
                     /locus_tag="VFQ61_39325"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET9960608.1"
                     /translation="MSETWSDSLTTVAYLVAGVLFILSLGGLSAQQSARRGNLFGILG
                     MAIAVLVTAVSHVQNYVAFLAPAAIGAAIGAALALRVAMTSMPELVAVLHSFVGLAAV
                     LVGFGSALMAGEVAPHETGAALVIHEMEVFLGVFVGAVTFTGSVVAFGKLRGMLRSKP
                     LLLPGRHLLNLVLLLACVALGIRFIGASPEQAVMLLGIQAAIASLIGVHIVMAIGGAD
                     MPVVVSMLNSYSGWAAAAAGFMLSNDLLIITGALVGSSGAILSYIMCRAMNRSFLSVI
                     FGGFGADEGAKPAAGAAKIEGEVVPVKAEDTATLLREAKNIIIVPGYGMAVAHAQYPI
                     FEICEWLRKRGKSVRFAIHPVAGRLPGHMNVLLAEAKVPYDIVLEMEEINEDFPTTDV
                     VLVIGANDIVNPGALDDASSPIYGMPVLEVWKAKTTIVMKRSMASGYAGVENPLFFHE
                     NTRMLFGDAKKNVDSILTVLRQGENAAAA"
     gene            complement(3825..4526)
                     /locus_tag="VFQ61_39330"
     CDS             complement(3825..4526)
                     /locus_tag="VFQ61_39330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET9960609.1"
                     /translation="MAEAAPIATTVEYRRPDGKTCAGYLVEPARAAVGTPALVLLEEW
                     WGLNAQIRGMAERFAAAGIRTLVPDLFRGRLAKDRAEASHMMSHLDFGDAASQDVRGA
                     LQYLKERSARVAVAGFCMGGALTLLAAASLRELDAAVCFYGIPPAEVFEPSQIRVPLQ
                     CHFAEHDDWCTPELVSSLEQRLVNGGVAHEIHRYDAAHAFMNAEQTKAYSEPHARLAW
                     ERAITFLHKHLGSRS"
     gene            4966..7059
                     /locus_tag="VFQ61_39335"
     CDS             4966..7059
                     /locus_tag="VFQ61_39335"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9960610.1"
                     /translation="MDPTKASNPSLLDELLGFESREHEDDDKTVVLDRQDSSTKLAVA
                     DVLEDPDGDRPTGVPPGVGVTAFAAVIVSDAVHELEPEALEDEPDSEALEEDDAEELE
                     PDALQADTDDALAQPELESVTEEPPTQMAPPVAPVFDAPVVAATAMPAVAAVAASPSA
                     SFGAGSPSGSMRAVEVSPTPSPVSQVPSSQISVPPPKPMRAMEASYSGAPGSVPQPLS
                     RNDLEDAWVGELIDGRYRVLRLLGVGGIGLVYLCRHELLEKPVALKVLRPEYVLHEDL
                     NERFMNEARAASSIKSPRIVDTLDVGVLPTGAPYFVMEYVEGETLASLLDREGHLSLA
                     RSVDVARQMAEGLEAAHAAGVVHRDLKPENVFLAHHAEGLYVKIFDFGIAKVASARKR
                     LTYVGAVFGTPHYMSPEQARGEQVDARSDLYALGIMLFEMVAGNVPFDGEEPLSVMAQ
                     HVDRAPPLMASIAGRPVPAPLEAIVARCLAKDPRDRYSSATELLFDLNTLSEPDLHHF
                     ELSYAQHAPMAAPPIEMGTPAPAVAFAHLPVDRPETLHAHVVPSAAPSGRKKGRMLWA
                     FMGLIALGGTGLLAFRHLSERPMIGAGLTAEARVKPSPAPVSNVETVENRPSEEGGIK
                     VDFVLAPIDAHLFLGEEDLGPMPVSIHVDKDKPVTLTVRRQGYVTRRVVVDGTETRIV
                     IGLVSVEKSSKRRKR"
     gene            7111..8787
                     /gene="ettA"
                     /locus_tag="VFQ61_39340"
     CDS             7111..8787
                     /gene="ettA"
                     /locus_tag="VFQ61_39340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HET9960611.1"
                     /translation="MPEFIFTMQEVSKVHPPDKKVLENITLAFFPGAKIGVIGSNGSG
                     KSSLLRIMAGVDKEFLGEARPHPGTKIGYFAQEPELHGFKTVKEAVDDAVSDIRKLVT
                     DYETVSMRLAEPMSDDEMNRVLEQQSTLQDKIDAVDAWSLDQRVELAMEALRLPPGEA
                     EIATLSGGERRRVALCRILLSRPDMLLLDEPTNHLDAESVAWLERYLKEFPGTVIAVT
                     HDRYFLDDVAEWILELDRGRGYPFKGNYSAWLDQKSARLAVEEKQESARQRKLKQELE
                     WVRASPRARQAKSKARLSAYEELLNQSSKGPADPTEIGIPPGPRLGDLVIEADHLRKA
                     FGDRLLIDDLSFKLPRSGIVGVIGPNGAGKTTLFRLLVGEQKVDGGGLKIGETVKIAY
                     VDQSRSALKPEASVWEVISGGEEKLLLGKREVASRAYCGWFGFRGSDQQKRVGSLSGG
                     ERNRVHLAKLLKEGGNLLLLDEPTNDLDVDTLRALEEALLSFAGCAVVISHDRWFLDR
                     VATHMLAFEGDSKVVWFSGNYQAYEEDRRRRMGEDADRPHRIKYKRLKLD"
     gene            complement(8868..>9485)
                     /locus_tag="VFQ61_39345"
     CDS             complement(8868..>9485)
                     /locus_tag="VFQ61_39345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960612.1"
                     /translation="DALASAKRERDGAMVPLVRRETVLLEATREPLRSEPPAWAGYFG
                     LVGLALGGAFAGLGQLGARVKAARVAFALSSGLLGLVTGLLGTIFMVFWLFTKHWSAY
                     RNENTLVCPPWALLLPVLCVGLALGRERASRRSLLVFQASAVSSIIALLLALIPHFGQ
                     DNTRIAALLGPVWLGLYAGACRLMRLPLWPPAKPARAKQLEPSKS"
BASE COUNT         1705 a         2885 c         3135 g         1760 t
ORIGIN      
        1 tgttcccgat cgatttcgtg atcaccgaca agttcgattt caaggcccgc accgtcggcg
       61 ccacgcgcgt cgtgggtcgc gacaagatcg agcccggcac cgaggcgctc gatatcggcc
      121 ccgagtcgat caaggcgttc gagaagattt tgctcggggc caagacgatt atttggaacg
      181 gtcccgtggg cgtgttcgag atcgacgcga cggccaaggg cacgatcgcc gtggcagaga
      241 ttctcgccaa ggcgacggcc gccggcgcga cgacgatcat cggcggcggt gactccgcat
      301 ccgcggtcaa gaaagcaggc gtggccagca aggtttcgca cgtttccacc ggtggtggcg
      361 catcactcga gtacctcgaa ggcaaggtgc tcccgggtgt tgccgccctg accaacgcct
      421 aattggcaaa cggctctcgg ccgagcatta ggcataaagc tgagagccga taaatccagc
      481 cgcatgccta aggctgggac ccaatcgagc agcatggctg atgagcgcag agttgcgctc
      541 atcagccgtc attgggggag gttggcgcca gcgcggtatg gtgggtttct cgagttgatt
      601 gggtgtacag tccaatgcga atcggggcct cgccatgaag attggtattc cgaaggagat
      661 tcgacctgcc gagcgccgcg cagcggcgac tccagatacg gtgcagcgcc tccgcaagct
      721 cggcttcgac gtagaagtcg aaaccggagc cggggcggga gcatctttcg atgatgcggc
      781 atatatggcc gctggcgcga ccatcgcggc ggacgccaaa tcgctgtgga gcaacgctga
      841 tctggtcctg aaggtgcagc cgcccgagct caacaccagc gatggtgtgc acgaggccga
      901 gttgctgcgc gaaggcgggc tgttgatcag cttcttgtgg cccgccaaga atgccgagtt
      961 gatcgagagg ctacgggccc gcaaaggcac cgtgttggct atggatcaag taccgcgcat
     1021 cacgcgggcg cagaagatcg acgctctgag ctcgatggcc aacatcgccg gttatcgcgc
     1081 ggtgatcgag gccgcgagct tttacggtcg attcttcacc ggacagatga cggccgcggg
     1141 caaggttccc cccgccaagg tgctcgtgat tggagccgga gtcgcgggtt tggccgcgat
     1201 tggcgcggcg cgtggcttgg gcgcgatcgt gcgcgcgttc gacacgcgat ccgaagtgcg
     1261 ggaccaagtg cagagtatgg gcgccgagtt cttggaggtc gaaatccaag aagagggcgg
     1321 gggcggtggt ggctacgcca aacagatgag ccccgagttc atcgccgccg aaatggcgtt
     1381 gtttttgcgc caggccaaag acgtcgacat catcatcacg acggcgctca tcccgggcaa
     1441 gcccgcgccg gtcttgatca ccgaggagat ggtgcgcgcg atgcgccccg gctcggtgat
     1501 cgtggatctt gcggctgaaa acggcggcaa ctgcgcattc acccggccgg gccaggtggt
     1561 ggtcgagagt ggcgttacgg tggtgggtta cgtggatctg ccgagccgct tggctccgct
     1621 tgcgagccaa ctctacggta cgaatctggt tcatctgctg acggacatgg gcggcaacgc
     1681 caacttccgt gtcgatctag aggatcaagt agtgcgcggc tcgatcgtat tgaaggacgg
     1741 cgagctgctg tggcctccgc ccaaggcgcc ccctccagcg gcgcccgtgt cgaagaagcc
     1801 cgaggcgaag atcgcggcgc atggccctgg gcacgccaag ccgacgcccg ccccgcttcc
     1861 gaaagccttc gttgccgcgg cttggctggt tggcgccatc gggattgggg cgctcggctt
     1921 actcgcaccg aaggagttct tgtcccatct cactgtgttc gtgctggcgt gcttcgtggg
     1981 ttggcaggtg atttggaatg tcacgcccgc cctgcacacc cctttgatga gcgtcaccaa
     2041 cgcgatcagc ggcatcatca tcgtgggcgg catgctgcag ctcacgttcg atttcagttc
     2101 tcccgtcacc tgggtgggag cgttttccgt gctgctggcc gcgatcaacg tggtcggcgg
     2161 gttcctggtc acacagcgca tgttgcgcat gttccaccgt tgaggccccc atgtcagaga
     2221 cttggtcaga cagtttgacg acggtcgcct acctggtggc gggcgtactc ttcattctca
     2281 gcctgggcgg cttgagcgcc cagcaatccg cgcggcgcgg caatctgttc ggcattctcg
     2341 gcatggccat cgccgtgctg gtgacggccg tgagccacgt gcagaattac gtcgcgtttt
     2401 tggctccggc cgcgattggg gccgcgattg gggcggcgct cgcgctgcgc gtcgccatga
     2461 cctcgatgcc ggagctggtc gcggttctgc acagcttcgt ggggttggcg gccgtgctcg
     2521 tgggcttcgg gagcgctttg atggcgggtg aggtggcgcc gcacgagacc ggtgctgcgc
     2581 tcgtcattca cgagatggag gtgtttctgg gcgtgttcgt gggcgcggtg acgttcaccg
     2641 gctccgtcgt cgcgttcggc aaactgcgcg gcatgctgcg gagcaagccg ttgctcttgc
     2701 cgggccgtca cttgctcaac ctcgtgttgc tgttggcctg cgtcgcgctg ggcatccgct
     2761 tcatcggcgc ttctccggag caggccgtca tgctgctcgg gattcaagcc gcgatcgcga
     2821 gtttgatcgg ggtgcacatc gtcatggcga ttggcggtgc ggacatgccc gtggtcgtat
     2881 ccatgttgaa cagctactcc ggttgggcgg cggcagcggc gggattcatg ctctcgaatg
     2941 acctgctgat catcacgggg gcgctggtgg gatcgagcgg cgcgatcctg agctacatca
     3001 tgtgtcgcgc catgaatcgc tccttcttga gcgtgatctt cggcggtttc ggcgcggacg
     3061 aaggcgcgaa acctgcggcg ggcgccgcga agatcgaggg cgaggtggtg ccggtgaagg
     3121 ccgaagacac ggctacgctg ctacgcgagg caaagaacat catcatcgtg ccgggttacg
     3181 gcatggcggt ggcccacgcg cagtatccga tcttcgagat ttgcgagtgg ctacgcaagc
     3241 gcggcaagag cgtgcgtttc gccatccatc ccgtggcagg gcgtttgcct ggacacatga
     3301 acgtgctctt ggcggaagcc aaggtgccat acgacatcgt gctcgagatg gaggagatca
     3361 acgaggattt cccgaccacc gacgtggtgc tcgtgatcgg cgcgaacgac atcgtcaatc
     3421 cgggcgcgct cgatgacgct tccagcccca tctacggcat gcccgtgctc gaggtctgga
     3481 aggccaagac cacgatcgtg atgaaacgca gtatggcgag cggctacgcg ggtgtggaaa
     3541 atcctctgtt tttccacgag aacacgcgca tgctgttcgg ggacgcgaag aagaacgtgg
     3601 actcgatctt gaccgtgctc cgccagggcg agaacgccgc cgcggcctga ccgcaagcgc
     3661 gacgagccgg cgaatcacga ccgcgaggga gcgtggccct cagcggtact cgctcgaggg
     3721 gcccgcacgg ccttgctccg cgattccgcg cagcgcgttg ccccggccac gacggcaacg
     3781 cgcccgcgta gatcttgctg tttcaaatcg gagctacgtc cccttcagga tcggctgccg
     3841 aggtgcttgt gcaggaaggt gatcgcgcgc tcccaagcca ggcgcgcgtg aggttcggag
     3901 taagccttgg tttgctcggc gttcatgaaa gcatgagccg cgtcgtaacg atggatttca
     3961 tgcgcgacgc cgccgttcac caagcgctgc tcgagtgacg ataccaactc cggggtgcac
     4021 cagtcgtcgt gttcggcgaa atggcattgc agagggactc ggatttggct cggctcgaac
     4081 acctcggcgg gcgggattcc gtagaagcac accgccgcgt ccagctcacg gagtgaagcg
     4141 gccgcgagca gcgtgagcgc gccgcccata cagaagcccg ctacggccac gcgcgctgaa
     4201 cgctccttga ggtactgcag cgcgccgcgc acgtcctggc tcgcggcgtc tccaaaatcg
     4261 aggtgcgaca tcatgtggct cgcttcggcc cgatctttcg ccaagcgccc ccggaataga
     4321 tccggaacca aggtccgaat accggccgca gcgaagcgct cggccatgcc gcggatctga
     4381 gcattgagcc cccaccactc ctccagcaaa accagagcgg gcgtgcccac cgcggctcgt
     4441 gcaggttcta ccaagtaccc tgcacacgtt ttcccatccg gccgcctgta ctcgacggtt
     4501 gtcgcgatcg gagctgcttc tgccatcaga ctaaccctcc ttagaagttg gatgcccccg
     4561 agggtcatcg gttctcgttc tgggatcaag gcgcctgttc caaccaaggc aggggcgcgg
     4621 agtgcgatgc agggataaag ttttggatct ggtgcagagc gcggcgtcgt gattaagaaa
     4681 cccgcgcgct cccaatctga aactcgggcg ggccgcttcg agggattctt tgaaactgac
     4741 gcttcatcac ccgtgtttcg cctgacgaat gtcgcggctc gctacagatc gctacaacga
     4801 cccatttctc tgagccaatc tgaagtcatc cagtatgctt tgaattggct cgcgggcctg
     4861 cattctgctt gcacgcccgg acctctccct catattcttc atctccatcc gaggcccccg
     4921 agcagtcggg gctcgcactg atcaacggcg tctgagttct tccgaatgga tcccacgaaa
     4981 gccagcaacc cctcgctcct cgatgagctg cttggctttg agtcgcgtga gcatgaggac
     5041 gacgacaaga cggtggttct cgatcggcag gattcgtcga cgaagctcgc cgttgcagat
     5101 gttctggagg atccggacgg cgatcgaccc acgggcgttc cccctggtgt gggagtgacg
     5161 gccttcgcgg ccgtaatcgt gtccgacgca gtgcatgagc tggaacccga ggctttggaa
     5221 gacgagcccg acagcgaggc gctcgaggag gatgacgcgg aggagctcga gcccgacgcg
     5281 ttgcaggccg atacggacga cgcattggct caaccagagc tcgaatccgt gaccgaagag
     5341 ccgccgaccc agatggcgcc gcccgtggct cccgtcttcg acgcgcccgt ggttgcagca
     5401 acggcgatgc ctgcagtcgc tgcggttgct gctagccctt cagcatcttt cggtgcgggt
     5461 tctccctcgg gatccatgcg ggctgtcgag gtttcaccga ccccatctcc ggtctcgcaa
     5521 gtcccgagct ctcagatttc cgtaccgcct cccaaaccca tgcgcgcgat ggaggcgagc
     5581 tacagcggcg ctcccggcag cgttccgcag ccgctctcgc gcaacgactt ggaagacgcg
     5641 tgggttgggg agctcatcga cgggcgctac cgcgtgctgc ggctgcttgg tgtaggcggc
     5701 atcggcctgg tttacctgtg ccgtcacgag ctgctcgaga agcccgtcgc gctcaaggtg
     5761 ttacgccccg agtacgtgtt gcacgaggat ttgaacgagc gcttcatgaa cgaagcgcgg
     5821 gcggcctcgt cgatcaagag ccctcggatc gtggatacgc tcgacgtcgg ggtgttgccg
     5881 acgggcgcgc cgtacttcgt gatggagtac gtcgagggag aaacgctggc ctcgctcttg
     5941 gatcgcgaag ggcatctctc gctcgcacgg tccgtcgacg tggcgcgcca aatggcggaa
     6001 gggctagagg cagcgcacgc ggctggcgtc gttcaccgcg atttgaagcc cgagaacgtc
     6061 tttctggccc atcacgcgga ggggctgtac gtcaagatct tcgactttgg gatcgccaaa
     6121 gttgcgagcg cacgcaagcg gctaacttac gtgggcgcgg tattcgggac gccgcactat
     6181 atgtctcccg agcaagcacg cggtgagcag gtcgacgcgc gttcggatct gtacgccctc
     6241 gggatcatgc tcttcgagat ggtcgccggg aacgtgccat tcgacggtga ggagccgctc
     6301 tcggtgatgg cccagcacgt cgatcgcgcg ccgccgttga tggcgagcat cgcgggtcgg
     6361 ccggtgccgg cgccgctcga agcgattgtg gcgcgctgct tggccaagga tccgcgcgac
     6421 cgttattcga gcgcgaccga gctgctcttc gatctcaata cgctgagcga gccggacctt
     6481 caccatttcg agctttcgta cgcacaacac gcgcctatgg ctgctccgcc catcgagatg
     6541 ggcacgcccg ccccggccgt ggcgttcgct cacctgccgg tcgatcgacc cgagacgctc
     6601 cacgctcacg tcgtgccctc ggccgcaccg agtgggcgca agaaggggcg gatgctgtgg
     6661 gcgttcatgg gcttgattgc gctcggaggc acgggcctac tcgcgtttcg ccacctgtcg
     6721 gagcggccca tgatcggggc gggtctcacg gcggaggcac gcgtcaaacc gtcgcccgcg
     6781 ccggtctcga acgtcgagac ggtcgaaaat cgcccgagcg aagagggggg catcaaagtc
     6841 gatttcgtgc tcgcacccat cgacgcccat cttttcctcg gggaagaaga tcttgggccc
     6901 atgccggtct cgattcacgt agacaaggac aagccggtca cgttgacggt gcgtcgacag
     6961 ggctacgtta cgcgtcgagt cgtggtcgac ggtacggaga cacgcatcgt catcgggctc
     7021 gtctccgtcg aaaagtcgag caagcggcgc aagcgctgaa tcagcgcaga ccgagctcga
     7081 atccggcgtg gcctttgata caagggaggc atgccggaat tcattttcac gatgcaggag
     7141 gtgagcaagg ttcaccctcc ggacaagaag gttctggaga acatcacctt ggcgttcttt
     7201 cccggggcca agattggagt gattggctcc aatgggtccg gaaagagcag cttgctccgc
     7261 atcatggcgg gggtggataa ggagttcttg ggggaggcgc ggccgcaccc tggcaccaag
     7321 atcggctact tcgcgcaaga gccggagcta cacggcttca agacggtaaa agaggcggtg
     7381 gacgacgcgg tgtcggacat ccgcaagctc gtgaccgact acgaaaccgt gagcatgcgc
     7441 ctcgcggagc cgatgtccga cgacgagatg aatcgcgtgc tcgaacagca gtccacgctg
     7501 caggacaaga tcgacgccgt ggatgcatgg agcctggacc agcgcgtgga gcttgcgatg
     7561 gaggcgctgc gcttgccgcc gggcgaagcc gaaattgcga ctctctctgg cggtgagcgg
     7621 cgccgggttg cgctgtgccg catcttgctc agccggcccg acatgctgct gctggacgag
     7681 ccgacgaacc atctagatgc cgagtcggta gcttggctcg agcggtattt gaaggaattc
     7741 ccgggcaccg tgatcgcggt cacccacgat cgctacttct tggacgacgt cgcggagtgg
     7801 attctggagc tcgaccgcgg ccgggggtac ccgttcaagg gcaactactc ggcttggctc
     7861 gaccagaaat cagcgcgcct cgcggtcgag gaaaagcaag agagcgctcg tcagcgcaag
     7921 ttgaaacaag agctcgaatg ggtgcgcgct tctccgcgtg cacgccaggc caagagcaag
     7981 gcccgcctct cggcgtatga agagttgctc aatcaatcga gcaagggccc cgccgatccg
     8041 actgaaatcg gtatcccgcc cggaccgcgc ttaggtgatt tggtgatcga ggccgatcac
     8101 ctgcgtaagg ccttcggcga tcgtctcttg atcgacgact tgagcttcaa gctgccgcgc
     8161 agcggcatcg tcggcgtgat tgggccgaac ggagcgggca aaaccacttt gttccgtttg
     8221 ctagttggag aacagaaggt cgacggcggg ggcttgaaga tcggagaaac cgtgaagatc
     8281 gcctatgtcg atcagtcacg ctctgcgttg aagcccgagg cttcggtgtg ggaggtcatc
     8341 tccggaggcg aagagaagtt gctgctcggt aagcgcgaag tcgcctcacg cgcgtactgc
     8401 ggttggttcg gattccgagg tagcgaccag caaaagcgcg tgggcagcct gtcgggcggt
     8461 gaacgcaacc gagtgcacct ggcgaagctc ctcaaagagg gtggcaacct gctgctcctc
     8521 gacgagccca ccaacgatct cgacgtagat acgctgcgag ccctagaaga ggcactcctt
     8581 tcgttcgcgg gctgcgccgt ggtaatcagc cacgaccgct ggttcttgga ccgcgtggcc
     8641 acccacatgc tggccttcga aggggacagc aaggtggttt ggttctcggg caactaccag
     8701 gcctacgaag aggatcgccg tcgccgcatg ggtgaagacg ccgaccgccc gcaccgcatc
     8761 aagtacaagc gattgaagct ggactgagcg gggctcagcc gttcttctaa ctctcgagga
     8821 accggaagag ctgcagcgat cggctcttcc ggtgtttgct cgccagctca gctcttcgag
     8881 ggctcgagtt gcttggcgcg cgcgggcttg gccggtggcc agagcgggag ccgcatcaac
     8941 cggcatgctc ccgcatagag gcccagccat accgggccga gtagggctgc gatgcgggtg
     9001 ttgtcttgtc cgaagtgcgg aatcagagcg agcaggagcg cgattatcga gctcacggcc
     9061 gaggcttgaa agaccaacaa cgacctgcgg ctggcgcgct ctcgtccgag cgcgagcccc
     9121 acgcacagca ccggcaggag tagcgcccac ggtgggcaaa ccagcgtgtt ttcgttgcgg
     9181 taagcagacc agtgtttggt gaacagccaa aacaccatga agatggtgcc gagcagcccc
     9241 gtcacgagcc cgagcagccc cgagctcaat gcgaacgcta ctcgtgccgc cttcacgcgc
     9301 gctcccaact gacccaagcc cgcaaaagcg ccgcccagcg cgagcccaac caggccgaag
     9361 tagccggccc aggcgggtgg ctcgctgcgt agcggctccc gtgtagcttc gagcaacacg
     9421 gtttctcgcc gcaccaacgg aaccatggct ccgtctcgct cccgcttcgc gctcgccaac
     9481 gcatc
//
LOCUS       DASTKU010001101         4175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1337045, whole genome shotgun sequence.
ACCESSION   DASTKU010001101 DASTKU010000000
VERSION     DASTKU010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337045"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="VFQ61_39350"
     CDS             <1..1618
                     /locus_tag="VFQ61_39350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET9960613.1"
                     /translation="KFIAIGDEEKRGEATLRLMREARAAAALNNEHVVRIYDVGESDN
                     GVPFIVMERLAGFDLAEIAKQAGALPVSAAVSFVRQACGALSEAHHVRIVHRDLKPSN
                     LFVVRRLDGTAGIKILDFGIAKSMAREGDRADSSTLTGAREALGSPHYMSPEQIRDAR
                     SVDTRADIWSLGAILFELIAGRPAFQAPTHPGLYAAIIADTPPSLSSLRPDVPQELAQ
                     IVARCLERKLDLRFQSVEELSSALEPFAAVSVAFDALPKPERPRAAQSTLSASGSRPA
                     LHEPTVTLSPDTPSSDERLAFGAASTQRAPSSPELFDDPGPRTERSPTSSEFSPSPVP
                     SASRNYRREGLTLFGLFTVAAFAYWILSRPGPSNVPEAPLTIKLELDSEPAGAHVLER
                     GELLGITPLTLQLSFANKLASPRALRLVLAGYKDFVVEQVPESGERAVHARLDRLASP
                     NPGAELKQPDASAPPNPSRGPAAPEAELKPVPVEGKPAGSAASPEVNKAAGGSARAVS
                     SPSASSPAPSAPALPAGAPGDWRPEIRTHR"
     gene            1729..>4175
                     /locus_tag="VFQ61_39355"
     CDS             1729..>4175
                     /locus_tag="VFQ61_39355"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9960614.1"
                     /translation="MARQSGSRIERLKPSGAAFACGMVIAALQLASCPARADDVADEA
                     DFLFQRGAEAYQHGDYATALERFLASNRLVPNRNVRFNVASTYARLGFYPEAFRYFTQ
                     VLAEEVEAEPRARIESALAVIRKNIAVLRIETQPSGATLYLDRRDLGARGTSPTELGV
                     APGSYRVLVELPGHYPAEFQVERAAAGETRSVQVKLVPMRGQLAIDGPRNANVKVVDA
                     SVERHCEAPCTLELSAGPHLVRVSLEGYREHEEEVDVPPAGKARLRVALEKLRGRLVV
                     TSDEPGALVEIDDAVAGFTPAVVSLPGGRHRVHLRKVGFREVRREVWVDPIRPATLEV
                     VLNEEEQVSSASRVQERVEDAPSSVSLLSQEEIRAFRYPTLADALRGVPGVYVWDDRS
                     YSAVGMRGIGQLGSYGNRVLVLFDGHPLNDNWVGSSYVGFDGHADLLDLEQIEIVRGP
                     GSVAYGTSAFAGVVNLVHRKAGRHSQITLGLGTNSDGVALGRARAEGPLGKNGGIWAS
                     AAVARGGGRDFHFPELASPAPQARDGSVRNRDGFESAGFQGSVQQGPLSVGWYWYTHD
                     KAFPTAAYDTLVGDPRSRQRDTRAALEVRMEPRISKSWKWLSRAHLNRYEFRGNYPLA
                     PADGGLTVDVFRGTWAGVEERLEYRPSARFRWLIGGELQSHFQVEHEALSGLDAKAES
                     AGVQKHSYRVGAGYSSLEWQAKPDLTLYAGARLDVYSTFGSALNPRLAAIYKGLPGYN
                     IKLLSSRAFRAPSVYELYYADGYSQRANPDLGPEHIDALEIEVTRELSPLWNASASGF
                     VNYLTDSIVPRAPENDLL"
BASE COUNT          679 a         1304 c         1388 g          804 t
ORIGIN      
        1 caagttcatc gctatcgggg atgaagaaaa gcgcggcgaa gcgacgctgc gcctgatgcg
       61 cgaggctcgc gcagcggcgg ctctgaacaa cgaacacgtg gttcgaatct acgacgtggg
      121 ggagagcgat aacggcgtgc ccttcatcgt catggagcgg ctcgcgggct tcgacttggc
      181 cgaaatcgcc aagcaggccg gagcgctgcc cgtatctgcg gccgtgagct tcgtgcgaca
      241 agcttgcggg gctttatccg aggcgcatca cgttcggatc gtgcaccgag acttgaagcc
      301 atcgaacctg ttcgtggtgc gtcgcctgga cggcacagct ggcatcaaga tcttggattt
      361 cggcatcgcc aagtcgatgg caagagaggg tgaccgcgcg gacagcagca cgctcacggg
      421 ggcgcgcgag gcgctgggtt cgcctcacta tatgtccccc gagcaaattc gggatgctcg
      481 ttccgtggac actcgtgccg acatttggtc gctcggcgct atcctgttcg agctgattgc
      541 cggacgcccg gcgtttcagg ctcccacgca tcccggcttg tacgcggcca tcattgccga
      601 cacgccaccg agcttgagct cgctgcgccc ggatgtgccg caggaattgg cccaaatcgt
      661 ggcccggtgt ctggagcgca agctcgatct acgcttccag agcgtggagg agctctcgag
      721 tgcgctcgag cccttcgcgg cggtcagcgt cgcgttcgac gctttgccca aacccgagcg
      781 cccgcgcgcg gcacaatcta cgctgtctgc gagcggctcc cgtccggcat tgcacgaacc
      841 gaccgtcacg ctctctccag acacgccgag ctcggatgag cggttggcgt tcggcgctgc
      901 gtcgacgcaa cgtgcgccat catcgcccga gctgttcgac gatccggggc cacgtaccga
      961 gcgttctccg acgagcagtg agttctctcc atcgccggtt ccgtccgcct cgcggaatta
     1021 tcggcgtgaa ggcctgacgt tgtttggctt gttcacggtg gccgcgttcg cgtactggat
     1081 attgagtcga cccggccctt cgaacgtccc cgaggctccg ctcacgatca agttggagtt
     1141 ggattccgaa cccgcgggcg cccacgtgct ggagcgaggc gagttgctcg ggattacgcc
     1201 gctcacgctg caactgagtt tcgcgaacaa gcttgcttcg ccgcgcgcgc tgcgcctggt
     1261 tctagccggt tacaaagact tcgtggtgga gcaggtgccc gagtccggtg agcgggccgt
     1321 gcatgctcgc ttggaccggt tggcgtcgcc gaatccgggc gctgaactga agcaacctga
     1381 cgcgagcgcg ccgcccaatc caagccgcgg ccccgcggcg ccggaagcgg agctgaaacc
     1441 ggtgccagtc gaaggtaagc cagctggttc agcggcttcc cccgaagtca acaaagctgc
     1501 tggcggttcc gcccgcgccg tctcgtcccc ttctgcgagt tccccggcgc cgagcgctcc
     1561 cgcgcttccc gcgggagcgc cgggggactg gcggcccgag atccgcaccc accgctgagc
     1621 cgttttcgga tccaccgtat ggtgcagcac acgtcgcgac aacatttccg acgccgggct
     1681 agtctgctgg ttccacgggt gtgagccgtt gtatgttctg cagtctcgat ggcacggcaa
     1741 tctggctcgc ggatcgagcg gctgaagccc agcggggcgg cgttcgcttg cggtatggtg
     1801 attgcggcgc tccaacttgc gagttgcccc gcgcgcgccg atgatgtggc tgatgaggcc
     1861 gactttctat ttcagcgtgg tgcggaggct tatcagcatg gtgactacgc gaccgcgctc
     1921 gagcgcttct tggcatccaa tcgcttggtg cccaatcgca atgtgcgctt caatgtcgcg
     1981 tccacttatg cgcggctcgg cttctatccc gaggcctttc gctatttcac ccaggtactc
     2041 gcggaggagg tagaagccga gccccgtgcg cgcatcgaat cggcgttggc ggtgattcgc
     2101 aagaacatcg cggttttgcg gatcgagacg caaccctcgg gtgccacctt gtatctggat
     2161 cgccgagatt tgggcgcgcg cggtacgagc ccgaccgagc tcggggtagc ccctggctca
     2221 taccgtgttc tcgtcgagtt gcccggccac tatcccgccg agttccaagt cgagcgggcc
     2281 gcggccgggg aaacgcgtag cgtacaggtg aagctcgtgc ccatgcgcgg tcagctcgcg
     2341 atcgatggcc cccgcaacgc gaacgtgaag gtggtcgacg ccagcgtgga gcggcactgt
     2401 gaggcgcctt gcacgctcga attgtcggcg ggtccgcacc tggtgcgcgt ctcgctcgag
     2461 ggttatcgag agcacgaaga agaggtggat gttccgcccg ctggcaaggc tcggctgagg
     2521 gtcgcgctcg agaagctgcg cggccgtctc gtcgtgacga gcgacgagcc tggcgcgcta
     2581 gtggagattg acgacgccgt agcgggtttc acgcctgcgg tagtttctct tccgggaggc
     2641 aggcatcgag tacacttgcg taaggtcggc tttcgtgagg ttcgcagaga agtgtgggtc
     2701 gacccgattc gcccggccac gctggaggtc gtgctcaacg aagaagagca ggtctcgtct
     2761 gcgtcccgcg ttcaagagcg tgtggaggat gctcccagct cggtgagcct gctgtcccag
     2821 gaggagattc gcgcctttcg atatccgact ctcgcagacg ccctgcgcgg cgtgcccggc
     2881 gtgtacgtgt gggacgaccg ctcttacagc gcggtgggca tgcgcggcat cgggcaactc
     2941 ggcagctacg gcaaccgcgt gttggtgctg ttcgacggtc atccactgaa cgacaattgg
     3001 gtcgggtctt cctacgtcgg cttcgacggc cacgcggact tgttggatct cgagcaaatc
     3061 gagattgtgc gcggaccagg ctcggtggcg tacggaactt cggccttcgc gggggtcgtg
     3121 aatctggtgc atcgcaaagc cggtcgacac tcccagatca cgctggggct gggtaccaac
     3181 agcgacggcg ttgcgctcgg tcgcgcgcgc gcggaggggc cgctcggcaa gaacggcgga
     3241 atctgggcct ccgcggcggt tgcacgcggc ggggggcgcg attttcactt tccggagctc
     3301 gcgagccccg caccccaagc gcgggacggt tcggtgcgca atcgagatgg cttcgagagt
     3361 gctggctttc agggtagcgt gcagcagggg ccgctctcgg tcggctggta ctggtacacc
     3421 cacgacaagg cctttccaac cgccgcgtac gacaccttgg ttggagaccc acgctcacgc
     3481 caacgcgata cgcgagcggc gctcgaagtg cggatggagc ctcggatctc gaagtcatgg
     3541 aagtggctgt cgcgcgctca cctgaatcgt tacgagtttc gaggcaatta cccactcgcg
     3601 cccgccgacg gcgggctgac cgtcgacgta tttcggggaa cctgggccgg cgtcgaagaa
     3661 cgcctggagt atcggccgtc cgcgcgcttt cggtggctga tcggcggtga gctgcagtcg
     3721 cacttccaag tcgagcacga ggccctgagc ggactcgacg cgaaggccga gtctgccggc
     3781 gttcaaaagc actcgtatcg agtcggggcg ggttactcgt ccttggagtg gcaagccaag
     3841 ccggatctga ccttgtatgc aggcgcgcgg ctcgacgtgt attcgacctt cggctccgcg
     3901 ctcaacccgc ggttggcggc gatctacaag ggtctacctg gctacaacat caagctactg
     3961 agctcgcgtg catttcgcgc ccctagtgtc tacgagctct actatgcgga cggttattcg
     4021 cagcgagcga atccggatct cggtcccgaa cacatcgacg ctttggaaat cgaggtcacg
     4081 cgcgagttat ctccgctgtg gaacgcgagc gcttcgggtt tcgtcaacta tctgaccgat
     4141 tccatcgttc ctcgcgcgcc cgagaacgat cttct
//
LOCUS       DASTKU010001102        26690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_441154, whole genome shotgun sequence.
ACCESSION   DASTKU010001102 DASTKU010000000
VERSION     DASTKU010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 26690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26690
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441154"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VFQ61_39360"
     CDS             complement(<1..960)
                     /locus_tag="VFQ61_39360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960615.1"
                     /translation="MRLTDEELKVISSPVFQRLRRIKQNGLLSYVFPAATHTRFEHSV
                     GVLFVVESMLQAIVANSIVANEKKELGTDSLSAVDIRNYRHNLAFLFRTARLAALCHD
                     LGHGPFSHAFDKFAPRRENVAALLEADSAIVNKRLADAIRSDTGKRVAHEWMSCVLFA
                     RLFEDLNGNVDDDTALVVAAAIHDSPELAPDPSTRSLIPLIHDLIASAPADADRMDYV
                     ERDSRSCGVTYGLFDRDRVLKSFLCYADEGKLRLGIKSSGFRAIENFVQARFQLFVQV
                     YYHKTNRAIELMLGKIGKIAEERDLCVMREDSLDSLIDDYVAIG"
     gene            complement(1278..1625)
                     /locus_tag="VFQ61_39365"
     CDS             complement(1278..1625)
                     /locus_tag="VFQ61_39365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960616.1"
                     /translation="MVEEPYRSTPETDELKTIRTSLFRAIGKATRVVLYGVSISGLDV
                     EFGQVLASALHGGSVHNVIVVKPTYPDVAERIATIVNDAERSTTHPLLRAVGSHISLG
                     VLGSYVDSRETPA"
     gene            1926..2150
                     /locus_tag="VFQ61_39370"
     CDS             1926..2150
                     /locus_tag="VFQ61_39370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960617.1"
                     /translation="MDDIRGGEPLKIQQRAGHTSFEMTQKNIRTAEAVREAIGDVFPE
                     LPKCLLAGANRLANRPDELQVLGTIVEAPG"
     gene            complement(2241..4124)
                     /gene="cysN"
                     /locus_tag="VFQ61_39375"
     CDS             complement(2241..4124)
                     /gene="cysN"
                     /locus_tag="VFQ61_39375"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HET9960618.1"
                     /translation="MITQETEFISQDILGYLDQHQKKELLRFVTVGSVDDGKSTLIGR
                     LLHDTHGVYEDQLKAVKRASTRAGLEIDFSLFTDGLKAEREQGITIDVAYRYFSTAKR
                     KFIIADTPGHVQYTRNMATGASTANVAIILIDARLGILQQSRRHAYIASLLGIPNLTV
                     CVNKMDLEGYSQPVFDAIATAFADFCSKLTFKEVKFIPVSALRGDNVVHRSGEMPWYQ
                     GPTLLEHLENVPVAQDRNLKDFRFPVQYVLRPDLDYRGFSGTIASGVVKAGDPVMVLP
                     SGKSTKIVAIDTADGELESAFAPMSVTLRLAEEIDISRGDMLVHPASRPSVGKRFEAM
                     LVWMSERALDLQKSYLLKHTTQMVRAEIEGVAFTTDLETLKQQPATRLELNDIGRVTI
                     SCHRPLFYDAYRDNRSTGSFILVDSLTNNTVAAGMILPGEASPRQGDSNAPQPERVHS
                     HVSHDERAERLGQKGCTVWLTGLPASGKSAIAYALERRLFDIRRLAMVVDPDDGISTG
                     QQPDGSSPWQTPELARRCTDAGLIVIFSYSSPLRADRAAIRDVVGDARFVEVHVDTKL
                     AVRKERDARGVYGPGHSQPSEEAPTSPDLVVSLNSGDPEEAADAIVKILVKRGLLPSL
                     YSL"
     gene            complement(4191..5138)
                     /gene="cysD"
                     /locus_tag="VFQ61_39380"
     CDS             complement(4191..5138)
                     /gene="cysD"
                     /locus_tag="VFQ61_39380"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HET9960619.1"
                     /translation="MLAVREAPSTAEQALDFRLSHLKQLEAESIHVLREVVAEFERPV
                     MLYSIGKDSSVMVHLARKAFHRGKLPFPLLHIDTTWKFKGMIEFRDRFCRENNFDLRV
                     HTNKQGLEDGINPFDHGSSKYTTIMKTHALLQALHDGGYDAAFGGARRDEERSRAKER
                     IYSFRDRYGQWDPKNQRPELWNLYNAKINKGESIRAFPLSNWTELDIWLYIYLENIPI
                     VPLYFSAPRPVVDREGTLVMVDDERMRLNPGEKPRMEMVRFRTLGCYPLTGAVRSNAT
                     TLPDIIREMLLTKHSERSGRLIDHDEEASMELKKREGYF"
     gene            complement(5326..6027)
                     /gene="aat"
                     /locus_tag="VFQ61_39385"
     CDS             complement(5326..6027)
                     /gene="aat"
                     /locus_tag="VFQ61_39385"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET9960620.1"
                     /translation="MTRHALPAILPRRGRPWFPSVNAADVHGLVAAGGSLSADWLLHA
                     YECGIFPWYDEDSPPLWWSPDPRAVMDLERLHVSRSMERFIRNTDLVLAFDTAFPDVM
                     RECGRDREDGTWILPETVEAYCRLHELGHAHSIEVWTPDRRLVGGLYGVHRGGLFAAE
                     SMFHRVTNASKLALIAAVRSLFQAGITLFDVQFVTPHLASLGAYTVPRAEYMRRLASA
                     TASHVSLGQLELAAT"
     gene            complement(6132..6539)
                     /locus_tag="VFQ61_39390"
     CDS             complement(6132..6539)
                     /locus_tag="VFQ61_39390"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET9960621.1"
                     /translation="MEKTTLTTETDGQVSLAEGVVLSPAAAAPSGEPSSHANRGLRIG
                     DVARRLGLSTHTIRAWERRHGLVRPLRSPNGERTYTEANVQHLLLIQETHKVGYALKD
                     IARLSTEELSEILARARALGETPAPPPAEPDPS"
     gene            complement(6551..7264)
                     /locus_tag="VFQ61_39395"
     CDS             complement(6551..7264)
                     /locus_tag="VFQ61_39395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960622.1"
                     /translation="MSLRVLSSAGANRAQAVNGSASNSVGQAVGHADDARAASPPRPA
                     VHPSFWQELVVYVGRMREFQRVDWVVYVSWVGLMFGLVIATLGFLLIGHAHGVSFPSE
                     AWLLPVGASIFAISIAIDTVGHRTIYKEEISRAEGLVHHITIFSGIGACVLLCMAHTY
                     PRGLWVPAMVLTALSFLYSLIDEAFHWRRYVQKFSDRVEMWSHVGILIGHGTLMTAWW
                     WWFFDGYAGVAATLKAISG"
     gene            complement(7261..8298)
                     /locus_tag="VFQ61_39400"
     CDS             complement(7261..8298)
                     /locus_tag="VFQ61_39400"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HET9960623.1"
                     /translation="MTLTARYDAEREALDPRQLCERVLAQGSKSFALASKLLPVRARA
                     DAATLYTFCRRVDDAIDEAPGDPEPALRELQLQLRAAYAGGPHRDVAWQAFSDLVRET
                     SIPENYPRELLLGMAMDVSGTRYESLDELLLYCHRVAGVVGLMMCHVLGLDRDSALTH
                     AAQLGLAMQLTNICRDVAEDFARGRVYIPASWLRGTPAEALLDAPKVSSSAVEREPRE
                     LPERARAALSRAVDRLLVVADALYAQSRSGLRSLPWRAAVSISVAGSLYAAIGRELRA
                     RECDVYAGRAVVPAPQKAWLLAEGAAFAASEGLRRRTRTARHRPPQRAVSFPEDVLLP
                     WEPLPKFGALS"
     gene            complement(8295..9854)
                     /locus_tag="VFQ61_39405"
     CDS             complement(8295..9854)
                     /locus_tag="VFQ61_39405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HET9960624.1"
                     /translation="MADGSAYSGRGHAGDNLPGKRAAIIGSGFGGLAAAIRLQASGFK
                     TTLFEARDKPGGRAYVYEQDGYVFDAGPTVITAPECIDELFTLAGRRREDYVELLPVN
                     PFYRLLWEDGTRFDYTGEAADMERQIGELSPRDVAGYRKFLKYTARVFEKGYTELAAT
                     PFLNFFDMVRVAPHLARLRADRSVYQTVSRFVQNPHLREALSFHSLLVGGNPFDTSAI
                     YTLIHHLERSFGVWFPRGGTGALVRGLVQLYRELGGELRLASPVQGIRVSERSGKTTH
                     FVRAGSSAQAPEEPFDLVVSNADLHHTYQKLYSDQPAAKRMQRKLESSEWSMSLFVLY
                     FGTNRAYDLAHHTILFGRRYEELLREIFDGPSLPRDFSLYLHAPSVTDASLAPPGGAT
                     FYVLSPVPHLGKAAIDWQKESPRYAARIYSALERYMPNLRQHLVTERWFTPQDFQTTL
                     GAFQGSAFSVAPRLTQSAYFRPHNRDDHIPGLYLVGAGTHPGAGLPGVINSAKATASL
                     VVQDFPVGVAP"
     gene            complement(9847..10881)
                     /locus_tag="VFQ61_39410"
     CDS             complement(9847..10881)
                     /locus_tag="VFQ61_39410"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET9960625.1"
                     /translation="MTQPAYLFEPRNDAVRLLEEELSDKSSTSLEEPLSNLDPELWQR
                     ALFGPLTDFLGRPGKEFRAHLVRLCWELAGRRQPPPPELPLLVEVLHAGSLIVDDIED
                     GSAYRRGGPALHVKYGVPIALNAANWLYFWPARLLSRLELPPATELALHRVIGRTLLA
                     CHEGQALDVATRVSELDQLHVPRVVRNTTLLKTGKLFELAASIGAIAGGAPSSTVRVL
                     AEFGRELGAGLQMLDDLGGLTSQKRCHKGHEDLIQGRATWPWAWAAERLPRASYQRLL
                     PLSREVVAREVHPEHLAIELVKAVGADAKQRVSQHLKDALNQLSRALEPSRAFDELSR
                     EISHLEASYG"
     gene            11075..11659
                     /locus_tag="VFQ61_39415"
     CDS             11075..11659
                     /locus_tag="VFQ61_39415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960626.1"
                     /translation="MVGSRHVMLRVFSQGDVAALDLAQLVRNGTRFFDAELNLRDADG
                     FRLAAPLATPASDPAQAHLREPNPSPATVSLSIELEVVARQLRDAFEVRGRLVQTRDL
                     EAAQRAEHRGQSGGMSQLAARCRGVFELTPFQAPIVEATRAGELLLAAVFASVCLGPV
                     LPEDESALFGVRGAVTRYEAIVGACLLRTNRNRS"
     gene            complement(11687..13981)
                     /locus_tag="VFQ61_39420"
     CDS             complement(11687..13981)
                     /locus_tag="VFQ61_39420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET9960627.1"
                     /translation="MLLVTGIVGFSTLAIVVWTSIQESTEHLRSVQQYIEEGITSKGM
                     VLTQNHALALRSMTLDNAFLDMQGLLAHAIEEDGDLVYGLYVNNEHEALAFGDRTTSG
                     MASVDREAYRKLGLTEPELLVQKPRWARAHRLGQELVEVAAPVLGEKGEVVGTLRYGL
                     STRRMQKALAGAKQESRARLLRSSALIASLLGSTLLLGSILGRIQAVRITRPIAALTR
                     SARELAAGNRSVHVAIASNDELAELGSSFNSMVKELDSTYRRLEEMNRTLEEKVVQRT
                     AELADKNRDMRLVLDNVDQGFVTLSPNGIMSKERSAVVSRWFGEETRAVSFWEYLQQQ
                     APNFGASFELGWDQLREGALPLEVCLAVLPERLSAADRTFSFRYIPFQDGANLAGVLV
                     VIADITERLAREREDAEHQELMQAFRQLTQDRTGFELFMRESGSLVDAVCSGRLDTEQ
                     VALKRTLHTLKGNAGSMGLTFIAELCHQLEDQLAETGAMRAELVAALGARFTAITEQL
                     ASSGAIFADRFIEVSEPEYVAVLSDLFDLGPQYRSLLDRVLSWRYEPAEKPLRRLAHQ
                     ARALARRLSKGEIKVEVAAECIRLSPEDFTPFFSEMIHPVRNAIDHGLESPEQREAAG
                     KPREGSLVFRAWQEADQLTFEIADDGAGIDWDAIAAKARAKGLPSITQTDLLDALCTD
                     GVSTRAEATSLSGRGVGMASFKQWVVAHGGRLEVRTKRGEGTTWLIHLPSKLQPQPSP
                     SGTSMQTLSAPQQRPTLRAGHRHS"
     gene            complement(14168..15196)
                     /locus_tag="VFQ61_39425"
     CDS             complement(14168..15196)
                     /locus_tag="VFQ61_39425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960628.1"
                     /translation="MALSRRDLLLATPALTAAVSCAPVTRNQRAPSSYVELAAVRPSS
                     NRGTVVVCMPNTSQTREVWAALSDELGRELELVAVQVEGENATAHIAEAVTRHRPTAL
                     VLMNNPTLTAYGQYQRQQPPRAHARPGIAVMASFLDERPHHLRDVTGISYEVPLITVI
                     TNLRKLIQSPIERIGVVRRNALSRFVGRQSALARREKIRVIEAEVGANPNPSELKRAL
                     RNLKQRCDAIWVLNDDHLLSQTLIAGGWLPGLNERPWVPVIVGAQSLVSPANSFGTFA
                     MLPDHTALGVQAANLVFDIANEDWSLPAGDDVELPLSTLTVVDLAQARERFSLREDAL
                     TQVDRILE"
     gene            complement(15187..15885)
                     /locus_tag="VFQ61_39430"
     CDS             complement(15187..15885)
                     /locus_tag="VFQ61_39430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960629.1"
                     /translation="MSFKSRTAQPAAALLALGMLLTRTARAKDSKLLVYLHVPLKQRA
                     LQSALQGALPGHTVLAVGRMSDFERSLGEGPDAVLTLPMVLTAHGLSARLQGHRQGAP
                     NERYALVAVDVAPTPSAVHTVGVLDLLGRDATTSFVHALIGAKPKVERVTKLEDLLPM
                     LQMQRVDAILLPARFVADLKEVSRLDLAYRELPTGVGLPALAGVDGRGEELAASVAKF
                     PLKLSKLLGVDEWR"
     gene            16048..18213
                     /locus_tag="VFQ61_39435"
     CDS             16048..18213
                     /locus_tag="VFQ61_39435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET9960630.1"
                     /translation="MRASTVTLALGAACWLTAGSAQAQSSPSKAPGAEAVEQQGIQDL
                     DLVKLLNVEVSTATKQAESLEEAPAVIQVVTRDDVRRWGYQSVAEVLQHTVGLYLIDD
                     HISPNVGVRGMSGGLGAESGVIKVMIDGRSVAYRTTSGNWLGVELIPLESVAQIEILR
                     GPASALYGADAFLGVVNIITEKPEELRPLRARFCVGSTESNPGGRFDVASGFRLGHFD
                     VLLGAAGEATDRSGLSLPSESPAPRLPTDIGGRRVALDLRRRSLVLQSRLGYRVQEAG
                     QLVLSAYASGLRRGGDFAQYAQLTNAPATDEGERGTQVALGQFRVNADGVWHAAAGLD
                     LAFSSTYSQGGVLPEDRIDVASPLYYVERRQSYRGVDALIEARWTPSPRFNLIVGAET
                     IADRERIDEPLRIDRLTDQPVSNVTNRRPTWSSFDLGAYVSANLKLIDPWLKLTGGIR
                     YDHHSVYEGQLTGRFGITSRWSRTLVAKLLYGSAFKAPSPYLLHASPLRLGDVIGNPN
                     LRPQQIHTLEYQISWKPSGFFGLTSAVSYNWLLDKAEFTPQGINLTARNVSEQRTLTF
                     EARADVRHYDDYAAYLALDLVHSVRELGQEGYRANLVGTRNVAYPPWLGRAGFTVSVP
                     SAPQVPLEVGAQSLVVGPRRAADTSIVEAGRSFSFSSYVMLDVSIATREIYLLPGHES
                     RFALRSRNLLLTRGPDPGFSGFEYPVTPAEIFLELSHTY"
     gene            18366..18875
                     /locus_tag="VFQ61_39440"
     CDS             18366..18875
                     /locus_tag="VFQ61_39440"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HET9960631.1"
                     /translation="MDFYQVTIPHTIKLLAKLETWLDKAAALADQKKFKADTLLQARL
                     APDQYPLVRQIQAACDQAKFSAAKLTGKQPPSHPDTESTFAEIRARIQSVREYLGTFK
                     PEDFAGAAERPCSHVWMQGKALRGRDYWLEMGQPNFFFHYTTAYAILRHNGVDLGKSD
                     YLTDLSFMN"
     gene            19106..19888
                     /locus_tag="VFQ61_39445"
     CDS             19106..19888
                     /locus_tag="VFQ61_39445"
                     /inference="COORDINATES: protein motif:HMM:NF018837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HET9960632.1"
                     /translation="MNDLPAPENPPIVAAVQRARSGLRRDVQALLEQLDDAELLVPLA
                     RDLPDAPEGERIELEGELSLVPHLLPDTEGQLFAPLFTHAEPVEPVASTLEWTTDGEA
                     LKLCAFPARIALEMALEVIDNGHVLGLVIDPGAESELCLTRAEVSSLLLGRAVPLLAY
                     VQNIPEEERGSTLVAEGAEPPPPELRSALDVWLAATPEVRTHRLERTFNPDRDLEPHL
                     TLTLRVPESANRSSLFEGVTGALSGTVPPPGYLDVIFEPCES"
     gene            19876..20787
                     /locus_tag="VFQ61_39450"
     CDS             19876..20787
                     /locus_tag="VFQ61_39450"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET9960633.1"
                     /translation="MRELSTSPSDVGQLQLMEGARPDLATDGASRLSWLERAQIRFVR
                     RTFDPGPVDRCVRACQRHIGSTWIHYCTRHLRSVHGLDRLPELRPEQSFVLVSNHRSF
                     FDLYVVMGDLVRRGLPHRIVFPVRSKFFYTSPAGLFVNGVMSFFAMYPPIFRERRQAA
                     LNPKSLDELIWLLRRGGVFAGLHPEGMRKLDDDPYTLLPAQRGVGRIIQGARVPVIPV
                     FINGLENDLPRQITGNFNATGRPILVVFGEPIDFRGLLEKPSSQRVHQAIADRTLEVI
                     AGLGREERELRSLLGAEPASLTSPARG"
     gene            complement(20807..21751)
                     /locus_tag="VFQ61_39455"
     CDS             complement(20807..21751)
                     /locus_tag="VFQ61_39455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960634.1"
                     /translation="MLREALIRVRGELSAMGRDATISTASSPSPPEPDARQSEAGLLV
                     VEVISNRVRIQAYGPYAATPIVQEFDLSRRDLTAEEVGVRTVEALRAALLGHPSEPSP
                     TPERAVKQPDETLPKPARVPSVLAPKKRADPCSLAPRSGNGLSLLAAPAVYFEPSSGM
                     TGLGAQGALLFGRSHFVAGVFAASSLVPGDIRDLVGKVDVGSRWGTARGGGTIALDGG
                     IELWFLAGAGVAQYTIRGRAEPGYVGVSRTHTSPVVAAAVGGAGWFTPSFGAYLLLDT
                     SAAIDSPSVHVAGRQIAELGRPSLWISAGIVLQAPRSQ"
     gene            complement(21969..23321)
                     /locus_tag="VFQ61_39460"
     CDS             complement(21969..23321)
                     /locus_tag="VFQ61_39460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9960635.1"
                     /translation="MSNPNQPSRPSRALQPEDVNAAFRALAEEHAEDREADDRERAWR
                     LIQIHAREDQDERRVPAFRYPVRTPLARVFGRFAVPAVAVAALAAAAFGARGLFSTPS
                     ENATLAASPSGAIHTEAASKNVDFGDGTRLIVAPHTSLSVEVLGAHASRTRLERGRLE
                     VDLHPRSDSEYRFFIGPYEVQAQAARFEVSWDPVQENFSLLSHGDNVRVLGPDGLNRT
                     VPSGQKLELSAAQSAVAGSSFDLQAPVVSVPGRLQGDSGRSAKNDPDRARIAARSERN
                     EARNERGEQRNERSEVAAPEDLDFASLVAKGRFAEVVSAAESQGLEQVLREKSASELN
                     ALGHAANYTGRSQLAVQAWTALRKRFAAHRTARQAAFFLGRIQEQQGRLNEALGWLDT
                     YLAEARKDSYVPEAAGRRLLLVQKLEGPLVAQKAAREYLERFPNGAYASNARALLSQP
                     "
     gene            complement(23314..23964)
                     /locus_tag="VFQ61_39465"
     CDS             complement(23314..23964)
                     /locus_tag="VFQ61_39465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET9960636.1"
                     /translation="MSKTASIRVLVPPLVSSKSLLPPRSSEDLHSAISGNSPDWQRRA
                     FDEFHGLVHGLVLKSLGPNAEASDLVSEVFVTFFESAHRIRTPTAVRSYLVSITMNAI
                     RREVRRRKRRRLFLKLSGLSPELESEPGRDDPKAKAALIQLSRILDELRVNDRMAFVL
                     HSLEGMQIAEIAAALDVSLSTAKRRVRRANEHVLKRVSRNALLADYVQERTRRGRV"
     gene            24282..24869
                     /locus_tag="VFQ61_39470"
     CDS             24282..24869
                     /locus_tag="VFQ61_39470"
                     /inference="COORDINATES: protein motif:HMM:NF025563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HET9960637.1"
                     /translation="MASSVLVLALGCNSTRVDAILALGEKPIQDCTETRGTLKAGTYR
                     VRSVDRSRCWRIGQETSIFGNLGYLIAETPECTLEGELWSFENSDSVPGAFEVHSTSH
                     SGFSLDVQESSKADGTNVITYGSHTLQNQRFYVREIEPGVYEFSPAHVMTSCISERNS
                     TSIGESAIQIEGCVPTASAQHWQILSDGCGGASGS"
     gene            25225..>26690
                     /locus_tag="VFQ61_39475"
     CDS             25225..>26690
                     /locus_tag="VFQ61_39475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960638.1"
                     /translation="MKRTCSLSLMGWVGTLVLGCTGTVSDPGAGATGGGAGTPNAGQP
                     NAGATHTTVNPDGSTTTTKPDGTTVTTMPDGSTVTMNPDGTTTTTPPGTGSGGTPGTG
                     SIEPGDVTQPATPFEAVTALTSVRKVKNLMTGLAPTDAEVQAVAERGAAGLQELIGKW
                     MNEADTQPKFRDKMLQFFRNAFQQTGFIPTEDFKHQLLENGGFDFGPLGTGAVGDDIY
                     FRLVQNIQDSFAMTAWQFVVDGRPFSEVLTTDRFMMTTALKAVYVQIEMPNDQPYASF
                     GQSASNFVKWSINMAADAVIPIETVVGNRAALQFDDQKPAGGGSGFSIGNTCQGTDVV
                     KEYSGYAQLFQRLIGYTPRNKFVANPECWEHASKPYFQVTDTQDWQWVKIKKMADGES
                     YIYPYDIPALRTASELSLKMPRVGFFTTPAFLALWNTNDSNQHRVTANQTLLVALGVS
                     YTPEAAFTPASVEGLAVLDKNHSVEGSECYACHRGLDPL"
BASE COUNT         4992 a         8288 c         8354 g         5056 t
ORIGIN      
        1 accaatagcg acatagtcgt cgattaggct gtccagcgag tcctctcgca tcacgcagag
       61 atcccgttcc tccgcgatct taccgatttt cccgagcatt agctcaattg cgcggttcgt
      121 cttgtggtag tacacctgaa caaacagctg gaatcgagcc tgcacgaagt tctcgatggc
      181 gcggaaaccg ctcgatttga tcccgaggcg cagctttcct tcatcggcat agcagaggaa
      241 gctcttgagc acgcgatccc gatcgaatag gccgtacgtg actccgcagg accgactgtc
      301 tcgttccacg tagtccattc gatcagcgtc cgcgggagcc gaggcgatca agtcgtggat
      361 cagcgggatt agcgagcgcg tagacggatc cggagcgagc tccggactat cgtggatcgc
      421 ggcggccacg accaacgctg tgtcatcgtc cacgttgcca ttaaggtcct caaagagtct
      481 cgcaaaaaga acacaagaca tccactcgtg agcaacgcgc ttgccggtat cgctacggat
      541 ggcgtcggcc aatcttttgt tgactatcgc gctatcagcc tcgagaagcg cagcgacgtt
      601 ctctcgccgg ggggcgaact tgtcgaaggc gtgagaaaac ggaccgtgtc caaggtcatg
      661 gcacagggcc gcaagcctgg cggttcggaa cagaaaggcc agattgtgac ggtaatttct
      721 gatatctact gccgacaaag agtcggtgcc gagttccttc ttctcattgg ctacgatgct
      781 gtttgcaacg atcgcttgca gcatggactc gacgacgaaa aggacgccaa cggagtgttc
      841 gaaacgtgtg tgcgtcgccg cgggaaacac gtagctcaaa aggccgttct gcttgatacg
      901 tcgcagcctc tggaagacgg gcgagctgat aacctttaat tcctcgtcgg tgagccgcac
      961 gagtccgtgg acaggatcca gaatcgatct cgtgagccga agagggtgac cgtccatgga
     1021 aatgaacatt agtcaagcct ggccggcgga agcaattgtt ctttaggcac gtgcctccac
     1081 gcttgtaggg tacggcatgc agggtcaatc gcggcgtaaa tgactcaata attccctgcg
     1141 tatagggccg ggctcatccg ccatgctccg agggcagtgc gagcgcggtc ccctggaagc
     1201 tcactaatgt acgtcaggtg cgagctttcg ccaagcgaac cagcagtcac gtaatccgct
     1261 gaccgacccc gtgctggtca agccggcgtt tctcgtgaat caacgtagct gccgagaaca
     1321 cccaaactga tgtgagagcc gacggcgcgt aggagcggat gggtggtcga ccgctccgca
     1381 tcgttcacga tggtcgcgat acgttcggcc acgtccggat aggtcggttt caccacaata
     1441 acattgtgaa cgctgccgcc gtgaagtgcg ctcgctagca cctggccgaa ctcgacgtcc
     1501 aatcctgata tgctgacgcc atagagcaca acccgtgtgg ccttgccgat cgctctgaac
     1561 agactggtac ggatggtctt cagctcatct gtctccggcg ttgacctgta tggttcttcc
     1621 accacctcgg ttggactcag acttgagctc aaagcgcaca cgtgggacga cgtcgacgtg
     1681 gagcgcggca tcatctccat acgccactcg ttcgaccgag agagcggcga gccgaaaggc
     1741 gcttcgtgag cgagcagaac ctattgccgc tgctgcgcgc gaggcgatca gaagtggtgg
     1801 gcggccgacc tccgaaagca tctgcgcgct gcgggcatca cgggcgcggc gctgttcgag
     1861 acggacgcga cccgcaagcg cttgcgctac ttgcgcttcc acgaactcca gagcacggtg
     1921 ctcacgtgga tgacatccgc ggcggcgagc cgctcaagat tcagcagcgg gctggtcaca
     1981 cgtcgttcga gatgacccag aagaacatcc gtaccgccga ggctgtccgc gaggccattg
     2041 gcgacgtgtt tccggagctg ccgaaatgct tactcgccgg ggcgaatcgt ctcgcgaatc
     2101 gtcccgacga gctgcaagtt cttggcacta ttgtggaggc tcccggctaa gaacccggtg
     2161 ccgcttccaa ttcgattgct tgaaactcga ggcaatgccc aattcgcgag cgggccgtcc
     2221 gctccgattc gaaggcgatt ttacagagag tacagcgacg gcaagagccc gcgttttacg
     2281 aggatcttga cgatggcgtc ggcggcctct tctggatctc ccgaattcaa tgacactacg
     2341 aggtcaggcg aggtgggcgc ttcctcgctc ggttgcgagt ggcctggacc gtacactccg
     2401 cgtgcatcgc gctccttgcg caccgcgagc ttggtgtcga cgtgcacctc gacgaagcgc
     2461 gcatcaccca ccacgtcgcg aatcgccgcg cggtccgcgc gcaatggtga ggaatacgag
     2521 aagatcacga tcaagcctgc gtccgtacag cgccgggcca attcgggtgt ttgccacggg
     2581 ctcgagccat ccggctgctg accggtcgag ataccgtcgt cggggtccac caccatcgcg
     2641 agcctgcgaa tgtcgaatag gcgccgctcg agggcgtagg cgatcgcgct cttgccggat
     2701 gcgggtaagc ccgtgagcca caccgtgcag cctttctgac cgagccgttc ggcgcgttca
     2761 tcgtggctga cgtgagagtg cacccgctcc ggctggggag cgttgctgtc gccttggcga
     2821 gggctagctt cgcccggcaa aatcatgccc gccgccaccg tgttgttggt gagcgagtcc
     2881 actaggatga aagagccggt gctgcgattg tcccgatagg cgtcgtagaa gagcggccga
     2941 tgacaggaga tcgtgacccg gccgatgtcg ttcagctcta gccgcgtcgc gggctgctgc
     3001 ttgagcgtct cgagatccgt cgtgaacgcg acgccctcga tctcggcgcg caccatttgc
     3061 gtggtgtgct tgagcagata gctcttctgc aggtcgagcg cgcgctcgct catccacacc
     3121 aacatcgcct cgaaccgctt gcccacgctg gggcgggacg cggggtgcac cagcatgtca
     3181 ccgcggctga tgtcgatctc ttcggccaaa cgcagcgtta cgctcatggg ggcgaacgcg
     3241 ctctcgagct cgccgtcagc cgtgtcgatg gccacgattt tggtgctctt gcccgagggc
     3301 agcaccatga ccggatcgcc cgctttgacc acgcccgaag cgatcgtccc cgagaagccg
     3361 cggtaatcta ggtccgggcg cagcacgtac tgcaccggga atcggaagtc tttcaggttg
     3421 cggtcttgcg cgaccggcac gttctccagg tgctcgagca gcgtggggcc ctggtaccaa
     3481 ggcatttcgc cgctgcggtg cacgacgttg tcgccgcgca gtgcgctcac tgggatgaac
     3541 ttcacctctt tgaaggtgag cttgctgcag aagtccgcga aggcggtggc aatcgcgtcg
     3601 aacaccggct ggctatagcc ctccaggtcc atcttgttca cgcacacggt cagattgggg
     3661 atgccgagca gcgacgcgat gtaagcgtgc cgacgggatt gctgtaggat gccgaggcgc
     3721 gcatcgatca agatgatcgc cacgttggcc gtggacgccc ccgtcgccat gttgcgcgtg
     3781 tattgaacgt gcccgggcgt gtccgcgatg atgaacttgc gcttggccgt ggagaagtag
     3841 cggtaagcga cgtcgatcgt gatgccctgt tcgcgctccg ccttcagccc gtccgtgaac
     3901 agcgagaaat cgatctcgag cccggcgcgg gtcgaagcgc gcttcaccgc cttgagctga
     3961 tcctcgtaca cgccatgggt gtcgtggagc aggcggccaa tcaaggtgga ctttccgtcg
     4021 tccaccgagc ccaccgtcac gaagcgcagc agctctttct tttggtgctg atccagatag
     4081 ccgaggatgt cttggctgat gaactcggtt tcttgcgtaa tcatggggaa acttcgggcg
     4141 cggcagaagg ggaagaattc gacgctagag acgggcggac ataccagggc tcagaagtag
     4201 ccctcgcgct tcttgagctc catcgaggct tcttcgtcgt gatcgatcaa gcggcccgaa
     4261 cgctcggagt gcttggtgag cagcatttcg cggatgatgt ccgggagcgt ggtcgcgttg
     4321 gatcgcaccg cgcccgtgag tgggtagcag cccagggtac ggaaccgcac catctccatg
     4381 cgcggcttct cgccggggtt caggcgcatg cgctcgtcat ccaccatcac caacgtgcct
     4441 tctcgatcca ccacggggcg tggcgccgag aagtacagag gcacgatcgg aatattctcc
     4501 aagtagatgt aaagccaaat gtcgagctcg gtccagttcg agagcgggaa ggctcgaatg
     4561 ctctcgccct tgttgatctt ggcgttgtag aggttccaca gctccggacg ctgattcttg
     4621 ggatcccact ggccatagcg gtcgcggaac gagtagatgc gctccttggc tcgggagcgc
     4681 tcttcgtcgc gccgcgcgcc gccgaacgcc gcgtcataac cgccgtcatg cagcgcttgc
     4741 aggagtgcat gcgtcttcat gatggtggtg tacttggagc tgccgtgatc gaacggattg
     4801 ataccgtctt ccaacccctg cttgttggtg tgcactcgca ggtcgaagtt gttctcgcga
     4861 cagaatcggt cgcgaaactc gatcatgccc ttgaacttcc aggtggtgtc gatgtgcagc
     4921 agcgggaacg gcagcttgcc gcggtgaaag gccttacggg ccaggtgcac catcaccgaa
     4981 gagtctttgc cgatcgagta caacatgacg gggcgctcga actcggccac gacctcacgc
     5041 aggacatgga tgctttccgc ttccagctgc ttgagatgcg aaaggcgaaa gtcgagggcc
     5101 tgttcggcgg tactaggagc ttcccggacg gcgagcatgg gcggcatata acaccaaaaa
     5161 catcggaatt aaggaaataa agataactta tcgagattgc tggaaaatcc aggttgagca
     5221 aaggtcgggt ttggctactc ctgcagcatg ctcgtcgggt tctccgaggc gcgatccggc
     5281 catcagggat cgacggcttc gaccgtgccg aggtgcttct cacggctagg tcgccgcgag
     5341 ctcgagctgt cccaatgaca catggctcgc tgtcgcactc gccaagcgac gcatgtattc
     5401 cgcgcgcggc acggtatacg cgccgagcga cgccaaatgt ggcgttacga actgcacatc
     5461 gaaaagggtg atgcccgcct gaaagagtga acgaaccgcc gcaatcagcg cgagcttgga
     5521 cgcgttggtt acgcggtgaa acatgctctc cgccgcgaac agaccaccgc ggtggacccc
     5581 atataacccg cccaccaacc gccgatccgg cgtccagacc tcgatactgt gggcgtggcc
     5641 gagctcatgc agacggcaat aggcttccac cgtctccggc aagatccagg ttccgtcttc
     5701 acgatctcgc ccacactcgc gcatcacgtc cggaaaggcg gtatcgaacg ccaacaccag
     5761 atccgtgttg cgaatgaagc gctccatgct gcgcgacacg tgaagccgct cgaggtccat
     5821 taccgcgcgc ggatcggggc tccaccaaag tggggggcta tcctcgtcgt accatgggaa
     5881 aatcccgcac tcgtaggcat ggagcagcca atcggcgctc aacgagccac ccgcggccac
     5941 caaaccatgc acgtcggctg cgttgacgga agggaaccag ggacggccgc gccgagggag
     6001 gatggctggg agagcgtgcc gcgtcactgc ttcgttcctc tgcgctggtt ccggtgcttc
     6061 ccccttatta ccagttagtg actcgttgct ctagctggtc ggtccgagcc cactgcgtcg
     6121 cgagggtttc gttagctggg atctggctcc gcgggcgggg gcgccggggt ttcccccaag
     6181 gcccgtgcgc gcgccagaat ctccgacaac tcctcggtgc tgagcctcgc gatgtccttc
     6241 aaggcgtaac ccactttgtg ggtctcttga atgagcagca agtgttgcac gttggcttcc
     6301 gtataggtgc gctccccgtt gggcgagcgt aacgggcgta ccaggccatg ccggcgctcc
     6361 caggcacgaa tcgtgtgagt gctgagcccc aagcgccggg cgacgtcccc gattcgtaac
     6421 cctcgattgg cgtgactgct cggctcgccc gaaggcgccg ctgccgccgg cgataggacc
     6481 actccctctg cgagggaaac ctggccgtct gtttcggtcg tgagtgtcgt cttttccacg
     6541 ttgctgctcc tcaacccgat atggctttca atgttgcggc cacccctgcg tacccatcga
     6601 agaaccacca ccaccaagcg gtcatcaggg ttccgtggcc aatcagaatc ccgacgtgac
     6661 tccacatctc cactcgatcg gagaactttt ggacgtaccg tcgccagtga aaggcttcat
     6721 cgatcaagct gtagagaaat gacaatgccg tgagcaccat cgcgggcacc cacagacctc
     6781 tcgggtaggt gtgagccatg caaagcagca cgcacgcgcc gatgcccgag aagatggtga
     6841 tgtgatgtac gagcccttcc gctcggctga tttcctcttt gtagatcgtg cgatgtccga
     6901 ccgtgtcgat ggcgatgctg atcgcgaaga tgctggctcc gaccgggagc agccaggcct
     6961 cgctcgggaa gctcaccccg tgcgcgtgcc cgatcagcag gaagcccagc gtggcgatca
     7021 ccaaaccgaa cattagaccg acccagctca cgtacacgac ccaatcgacg cgctgaaact
     7081 cgcgcatacg accgacgtaa accacgagct cctgccagaa gctcgggtgg accgcaggcc
     7141 gcggagggga ggcggcgcga gcgtcatccg cgtgtccaac cgcttgtcca acgctgttcg
     7201 acgcggagcc gttcacggct tgggctcggt tggcgccggc ggacgagaga acacgcaaac
     7261 tcatgacaaa gctccgaact tcggtaaggg ctcccaaggc aagagcacat cctctggaaa
     7321 gctcaccgcg cgttgcggtg ggcgatgacg cgccgtgcgg gtccgacgtc gtaagccctc
     7381 gctcgccgcg aaggcagctc cctcggcgag cagccacgcc ttctggggcg ccggaactac
     7441 cgcgcgcccc gcgtaaacat cgcactcgcg agcacgcagc tcgcgaccaa tcgccgcgta
     7501 caaggagcct gcgacggaaa tagaaaccgc ggctcgccaa ggtaagctcc gcagccccga
     7561 gcgcgattga gcgtagagag catctgcaac caccagcagt cgatcgacgg ctctcgacag
     7621 cgcggcgcgc gccctctctg gtaactcgcg cggctctcgc tcgaccgctg acgatgacac
     7681 cttcggcgcg tccagcagtg cttcggccgg ggtgccgcgc agccaactgg cgggaatgta
     7741 cacgcgcccg cgtgcaaagt cctcggccac gtcacgacag atgttggtga gttgcatggc
     7801 gagccccagc tgggcggcgt gggtcagcgc ggaatcccga tccaagccga gcacgtggca
     7861 catcatcagt cccaccactc ccgcgacgcg atggcaataa agcagaagct cgtccaagct
     7921 ctcgtagcga gtgccgctca cgtccatggc catgccgagc aacaactccc ggggataatt
     7981 ttcggggatg ctcgtttcgc gcaccaggtc cgagaacgcc tgccaggcca cgtcgcggtg
     8041 cggtccgccc gcgtaggcag cccggagctg gagctgcagc tcgcgcaggg ccggctcggg
     8101 atcaccgggg gcctcgtcga tggcgtcgtc cacgcgacga cagaacgtat agagtgtggc
     8161 cgcatcggcg cgggctcgga ccggcaggag cttcgacgcg agcgcgaagc tcttggagcc
     8221 ttgggcgagt actcgctcgc acaactgccg tggatccaag gcctcccgct cagcgtcgta
     8281 tcgagcggtc agagtcatgg cgcaaccccc accgggaagt cctgcacgac gaggctggcc
     8341 gtagctttgg cggagttgat cacgccgggc agccccgcgc ccggatgcgt tcccgccccc
     8401 accaagtaaa ggccaggaat atggtcatcc cggttgtgcg ggcggaaata ggcactctgg
     8461 gtcagccgtg gcgcgaccga gaacgccgaa ccctggaatg cacccaacgt ggtttgaaaa
     8521 tcttgcggtg tgaaccagcg ctcggtcacc aggtgttgac gcaggttcgg catgtagcgt
     8581 tcgagggccg agtagatgcg cgcggcataa cgcgggcttt ccttttgcca atcgatcgcg
     8641 gctttgccga ggtgaggtac ggggctcagc acgtagaacg tcgcgccgcc gggtggtgcc
     8701 aacgaagcgt cagtgacgct cggagcatgc aggtacaagc tgaaatcccg cggcaaggaa
     8761 gggccgtcga agatctcccg cagcagctcc tcgtagcgcc tgccgaacag aatcgtgtgg
     8821 tgagccaggt cgtaagcgcg gttcgtgccg aagtagagga cgaacagcga catggaccac
     8881 tcggagctct ccagctttcg ctgcatgcgc ttcgccgcgg gctgatccga gtagagcttt
     8941 tggtacgtgt ggtgcaggtc cgcgttggac acgaccagat cgaacggctc ctccggggct
     9001 tgcgcggaag agcccgcgcg tacgaagtgg gtcgttttgc cggaacgctc ggagacgcgg
     9061 attccctgca cggggctcgc cagacgcaac tctcccccga gctctcggta tagctgcacc
     9121 aatccgcgaa cgagcgcgcc ggtcccgccg cgcgggaacc aaaccccgaa gctgcgctcc
     9181 aagtgatgaa tcagagtata gatggcgctc gtatcgaacg ggtttccacc gaccagcagg
     9241 gagtggaagc tgagggcctc tctcagatgg gggttttgta cgaaacgcga tacggtctga
     9301 tatacggagc ggtccgcgcg cagccgcgct aggtgcggag ccacccgcac catatcgaag
     9361 aaattgagga acggagttgc ggcgagctcg gtgtagccct tctcgaatac gcgcgccgtg
     9421 tacttcaaga acttgcgata gcccgccacg tcgcggggtg agagctcgcc gatttgtcgc
     9481 tccatgtcgg cggcttcgcc cgtgtaatcg aagcgggtgc cgtcttccca gagcaatcga
     9541 tagaaaggat ttaccggcaa cagctcgacg tagtcctcgc gccggcgtcc cgcgagcgtg
     9601 aacagttcgt cgatgcactc gggcgcggtg atcacggtcg ggcccgcgtc gaacacgtaa
     9661 ccgtcttgtt cgtagacgta ggctcgacca cctggtttgt cccgggcctc gaacagggtc
     9721 gttttgaagc cactcgcctg taatcgtatg gccgccgcga gcccaccgaa gccgctgccg
     9781 atgatggcgg cgcgcttgcc cggaaggttg tcgcccgcgt ggccccgtcc cgagtatgca
     9841 gatccgtcag ccatagctcg cctctaggtg tgaaatttcg cggctcagtt cgtcgaaagc
     9901 gcgactcggc tcgagagctc gagagagctg attcagcgcg tctttcaggt gttgagaaac
     9961 tcgctgcttg gcgtccgcgc ctaccgcttt gaccagctcg atggcgagat gctccgggtg
    10021 cacttcgcgc gcgaccacct cacgcgagag cggcaatagc ctctgatagg aggcgcgcgg
    10081 caaacgctcg gcggcccagg cccaaggcca tgtcgcccgt ccttgaatca agtcttcgtg
    10141 ccccttgtga caacgttttt ggctcgtcag gccgcccaaa tcgtcgagca tctgcagccc
    10201 cgcacccagt tctcgcccga actccgccaa cacgcgcacg gtgctggatg gcgctccgcc
    10261 cgcaatggcg ccgatcgacg cggccaattc gaacagtttt ccggtcttca gcagcgtcgt
    10321 gtttcgaacc acgcgcggta cgtgcagctg atcgagttca gacactcgag tcgcgacatc
    10381 gagtgcttgg ccctcgtgac acgccagcag agtgcgcccg atcacacgat gcagcgcgag
    10441 ctcggtggcg ggcggcaact cgagccgaga cagcaaccgt gccggccaaa aatacagcca
    10501 attggcggcg ttcaacgcga tcggaactcc gtatttgacg tgcagcgcgg gcccgccgcg
    10561 tcgataggca gagccgtcct cgatatcgtc cacgatcaac gaaccggcgt gcagcacctc
    10621 caccaatagg ggcagttcag ggggaggggg ctgccggcgc ccagcgagtt cccaacacaa
    10681 gcgaacgaga tgcgcgcgaa actctttgcc cggtctgccc aagaagtccg tgagcgggcc
    10741 gaacaaggca cgctgccaga gttcggggtc caggttcgac agcggctcct ccaggctcgt
    10801 gctcgacttg tcgctgagct cctcttctaa gagccgaaca gcgtcatttc ttggttcgaa
    10861 cagatatgcg ggttgagtca tgagtcgttc cggggcgttg ggcctgcccc ttaggtctcg
    10921 tgttcagctt gaccgggttt tgaccaacgt ttgtctaagg tctgggttcg tggcaggcgc
    10981 tcgtcaaccc cgtgatggtg cgcaattcac gctatgcacg atttccgttt aagttttgtc
    11041 caacgtgggt ctaagcccct gatcacctgc gccggtggta ggctctcggc acgtcatgct
    11101 gcgggtcttt tcgcagggcg atgtggcagc gctcgacctc gcccagttgg tccgaaacgg
    11161 gacgcgcttc ttcgacgcag agctcaacct gcgggacgcg gacgggtttc gactcgccgc
    11221 cccactcgcg acgcccgcgt cagaccccgc gcaagctcac ttgcgggagc ccaacccgag
    11281 cccggcgacg gtcagcctgt cgattgaact cgaagtggtc gcgcggcagc ttcgcgacgc
    11341 gttcgaggtg cgaggtcgcc tcgtgcagac tcgggatcta gaagcggcgc agcgcgccga
    11401 gcaccgcggt caatccggag ggatgtcgca gctcgccgcg cgctgccggg gcgtgttcga
    11461 gctcacgccg ttccaagcgc caatcgtcga ggccacgcgg gccggggagc tgctgcttgc
    11521 cgctgtgttc gcttccgtgt gcctcggacc cgttttgcca gaggacgaga gtgcgttgtt
    11581 tggggtgcgc ggcgccgtta ctcgctatga agccatagtg ggcgcgtgct tgctgagaac
    11641 caatcgcaac cggagctagt cgccgccggt cgattgctcg tgtctctcag ctgtgccgat
    11701 gaccggccct caacgtgggt cgttgttgtg gtgcggacag cgtctgcatc gaagtgccac
    11761 tcgggctggg ttgcggttgc agcttactgg gcaaatgaat cagccaagtc gtgccttcgc
    11821 cgcgcttggt gcggacttcc aggcggccgc cgtgcgcgac cacccattgc ttgaaggagg
    11881 ccatgccaac accgcgtccc gagaggctgg tcgcttcggc gcgggtgcta actccatcgg
    11941 tgcagagcgc gtcgagtagg tccgtttgag tgatgctagg cagccctttg gcccttgctt
    12001 tggccgcaat cgcatcccag tcgatgcccg ctccgtcgtc cgcgatctcg aacgtgagct
    12061 ggtcagcctc ttgccaagct cgaaacacca gtgagccttc gcgcggtttg cctgcggctt
    12121 cacgctgctc ggggctttcg aggccgtgat cgatcgcgtt gcggaccggg tggatcatct
    12181 ccgagaagaa cggcgtaaag tcttcggggg agagccggat gcattctgcc gcgacttcca
    12241 ccttgatctc gcctttggaa aggcgacgcg cgagcgcgcg cgcctgatga gccaagcgtc
    12301 gcagcggttt ctctgcgggc tcgtaacgcc aggacagcac tcgatcgagc aagctgcggt
    12361 attgagggcc cagatcgaag aggtcggaca acaccgccac gtattccggt tctgacacct
    12421 cgatgaagcg atcggcgaaa atcgcgcccg agctcgcgag ctgctccgta attgctgtaa
    12481 agcgggcgcc cagcgccgcc accagctcgg cgcgcatcgc tccggtctcc gcgagctggt
    12541 cctcgagctg atggcaaagc tcggcgatga acgtaagccc catcgatccg gcgttgccct
    12601 tgagggtgtg cagcgtgcgc ttcagcgcaa cttgttccgt gtcgaggcgc ccgctacaga
    12661 ccgcatcgac caagctgcca gattcacgca tgaacagctc gaatccagtc cggtcctggg
    12721 tgagctgccg gaacgcctgc atcagctcct ggtgctcggc atcttcacgc tcgcgggcca
    12781 gacgctcggt gatatccgcg atcacgacga gaacgccggc caagtttgcg ccgtcctgaa
    12841 atggaatgta gcgaaagctg aaggtgcggt ccgcagcgga gagccgctcc ggcagcaccg
    12901 ctaggcaaac ctcgagcggt agagctccct ctcgcagctg gtcccagccg agctcgaaag
    12961 aggctccgaa gttcggggct tgttgctgca agtactccca gaaggacacg gcgcgggtct
    13021 cttctccgaa ccacctggag accacggccg agcgctcttt ggacatgatg ccgttcggag
    13081 acaaggtgac gaagccttgg tcgacgttgt ccaataccag tcgcatgtca cggtttttat
    13141 cggcgagctc cgcggtacgc tggacgacct tctcttccag agttcggttc atctcttcga
    13201 gccgacggta ggtcgagtcg agctctttca ccatgctgtt gaaggaactg ccaagctccg
    13261 cgagctcgtc gttcgaagcg atagccacgt gcacgctgcg gttgcctgcc gctagctcgc
    13321 gagcggaccg ggtcagcgcc gcgatggggc gcgtgatgcg tacggcctga atccggccca
    13381 ggatcgagcc cagcagcaaa gtggagccga gcagggaagc aatcaacgcg ctggatctga
    13441 gcagccgggc gcgcgactcc tgtttggcgc ccgccaacgc cttctgcatg cggcgagtag
    13501 agaggccgta acgcagagtc ccgaccactt ctcctttctc gcccagcacc ggcgcggcca
    13561 cttccacgag ctcctggccg agtcgatgag cacgagccca acggggtttc tgaacgagca
    13621 gctccggctc ggtcagaccg agcttccgat aggcctcgcg atcgacgcta gccatgccag
    13681 aggtggtgcg gtcgccgaac gcgagcgcct cgtgctcgtt gttgacgtac agtccgtaca
    13741 ccaagtcgcc gtcttcttcg atggcgtggg ccagcaatcc ctgcatgtcg aggaaggcgt
    13801 tatcgagcgt catgctgcgc aacgcgagcg catggttttg ggtcagcacc atgcccttac
    13861 tggtgatgcc ctcctcgatg tattgctgca ccgagcgcag atgctcggtg gattcctgga
    13921 tgctcgtcca caccacgatc gccagggtgg agaagccgac gatgcccgtt actaggagca
    13981 tcgttcgaac gaacttgcta cgaaggctct tgaatttggc cactgctctc gtcactgcgc
    14041 gtggttttat ttcgccccat cgacctcgca ccattacggc gtaagggggc gcaatgtcct
    14101 agacggccaa gatccgtctc agcttagggc agcttcgcga agtcccgcct ttcgcgcctg
    14161 gagaacatca ctccaaaatg cgatcgactt gcgtcaaagc atcttcgcgc aggctgaaac
    14221 gctcgcgtgc ttgcgccaga tcgaccacgg tcagggtgga cagaggtagt tcgacgtcat
    14281 cgcctgcggg taaagaccag tcttcgttgg cgatgtcgaa aacgagattc gctgcctgga
    14341 caccgagcgc ggtgtggtcg ggcaacatcg caaaggttcc gaacgagttg gcaggcgaga
    14401 ccaaggactg ggccccgacg atgactggca cccagggccg ctcgttcaag ccgggcaacc
    14461 agcctcccgc gatcagggtt tggctgagca agtgatcgtc gttgagcacc caaattgcgt
    14521 cgcaacgctg cttcaagttg cgcaacgcac gtttgagctc ggacgggttt gggttggcgc
    14581 ccacctcggc ttcgatcaca cggatcttct cgcgccgggc cagcgccgac tgcctaccta
    14641 cgaacctact cagcgcgttg cgccgcacga caccaatgcg ttctatgggt gattggatca
    14701 gcttacgcag attcgtaatc accgtgatga ggggtacttc gtagctgatg cccgtaacat
    14761 cgcgcaaatg gtggggccgt tcgtcgagaa aggaggccat gacggcgatt ccgggcctgg
    14821 cgtgggcgcg gggcggctgt tgtctttgat attgcccgta ggcagtaagg gtggggttgt
    14881 tcatcaacac cagagccgta gggcggtggc gcgtgaccgc ttcggcaata tgagccgttg
    14941 cgttctcgcc ttccacttgc accgcgacca attcgagttc ccgccccagc tcgtcggaga
    15001 gcgctgccca gacttcacgg gtttggctcg tgttcggcat gcacactact acggtgcccc
    15061 ggttgctcga ggggcgcacg gccgcgagct cgacgtaact cgagggggca cgctgattcc
    15121 gagtgaccgg tgcgcacgaa actgcagccg tcaaagccgg tgtcgcgagc agcaaatctc
    15181 ggcgactcag cgccattcgt ccactcccag cagcttggag agcttgagcg gaaacttggc
    15241 gacgctcgcc gcgagctcct cacctcgacc gtccacaccg gctagcgcag gtagccccac
    15301 gcccgtcggc agctcgcggt acgccaagtc gagtcggctg acttccttta ggtccgcgac
    15361 gaagcgtgcg ggcaacagga tcgcatccac tcgctgcatc tgaagcatgg gcaaaagatc
    15421 ttccagcttg gtgacccgct ctaccttcgg ctttgctccg atcagagcgt ggacgaagct
    15481 cgtcgtcgcg tcgcgcccca ggagatccag cacgccgacg gtgtgtacgg cgctcggcgt
    15541 aggagctaca tccactgcca ccagggcgta cctctcgttg ggcgctcctt gccgatgccc
    15601 ttgcagtcga gcactcagcc cgtgcgcggt cagcaccatt ggcaacgtca aaacggcgtc
    15661 tgggccttct cccagcgagc gttcgaaatc cgacatacgc ccgaccgcca acaccgtgtg
    15721 gcccgggagc gcaccttgca acgcgctctg cagcgcacgc tgcttcagcg ggacgtgcaa
    15781 gtaaaccaat aacttgctgt ccttggctcg agccgtgcgt gtaagtaaca tcccgagagc
    15841 aagcaaagcg gctgcaggct gtgcagtgcg ggatttgaag ctcatgcggg aatgaaaccc
    15901 tcggtacgcg cgcgttcgaa cagggcttgg acgagtaacg tcgcctcgcg ctcaacttta
    15961 agcgagtgta gggggcaggg cgagtcgtgc tcgaggctgg aacatgaaga ataccgaacc
    16021 tcgtccgtac gatggctagc aatgctcatg cgcgcttcga ccgtaacgct ggcgctgggc
    16081 gcggcgtgct ggctcacggc aggctcggcg caagctcaga gctcgccttc gaaagcgccg
    16141 ggagccgaag cggtcgagca acaaggtatc caggatctcg atctcgtaaa actgctgaat
    16201 gtggaggttt ccaccgcgac caagcaggcc gagagtctgg aagaagcgcc ggcggtcatc
    16261 caggtcgtta ctcgtgacga tgttcgacgc tggggatatc aaagcgtcgc ggaggtgctg
    16321 cagcacaccg ttggcctcta tctaatcgac gatcacatct ctccgaacgt gggtgtgcgc
    16381 ggcatgagcg gtggtctcgg cgcggaaagc ggtgtcatca aggtgatgat cgatggccgc
    16441 tccgtggctt atcgcaccac gtccggaaac tggctcgggg tggagctgat tccgctcgag
    16501 tcggttgcgc agatcgagat tttgcgcgga ccggcgtcgg ccctgtacgg agccgacgcc
    16561 ttcctgggtg tggtcaacat catcactgag aagcccgaag agttgcgacc tctgcgcgcc
    16621 cgcttctgcg tcgggagcac cgaatccaat ccgggtggcc gcttcgacgt agcgagtggt
    16681 ttccggttgg ggcacttcga cgtgctgctg ggcgcggcgg gtgaagctac tgaccgctct
    16741 ggtctgagct tgccttcgga atctccggcg ccccgcctcc ccacggatat tgggggacgc
    16801 cgggttgcac tggacttgcg tcgacgctcg ttggtcttac agagccgcct cggctatcgg
    16861 gttcaggaag cgggacagtt ggtgttgagt gcctatgcgt ccggtttgag gcgcggcggc
    16921 gactttgcgc aatacgccca gctcaccaac gcacccgcga cagacgaagg cgaacgcggc
    16981 acgcaggtcg cgcttggcca atttcgcgtg aacgcggacg gcgtgtggca tgcggcggcc
    17041 gggctggatt tggcgttctc gagtacctac tcacaagggg gagtacttcc ggaagatcgc
    17101 atcgacgttg cgagcccgtt gtattacgtg gagcgtcgcc aatcgtaccg cggggtggac
    17161 gcgttgatag aggcacgctg gaccccttcg cctcgcttca acttgatcgt tggcgcggag
    17221 acgatcgccg accgtgagcg catcgacgag cctctacgca tcgatcgcct caccgatcag
    17281 cctgtcagca acgtcacgaa tcgaaggcca acctggtcct ccttcgattt gggggcctac
    17341 gtgagtgcca acctgaagct gatcgaccct tggctcaaac tgaccggtgg cattcgctac
    17401 gatcaccaca gtgtctacga ggggcagctg acgggaagat ttggcatcac ttcacgttgg
    17461 agccgcacgc tcgtggcgaa gctgctgtac ggcagcgcgt tcaaggcgcc atcgccgtac
    17521 ttactccacg cctcgccttt gcgtttgggt gacgtgatcg gcaacccgaa cttgaggccc
    17581 cagcaaatcc acacactcga atatcaaatc tcctggaaac ccagtggctt cttcggtttg
    17641 acctctgcgg tctcctacaa ttggctgctg gacaaggccg agtttactcc gcaaggcatc
    17701 aacctcacgg cgcgcaacgt gtcggagcaa cggaccctga cattcgaggc gcgcgcggac
    17761 gttcgacact acgacgatta cgctgcctac ttggcgttgg atctcgtgca ctccgtccgt
    17821 gaactgggcc aagaaggata tcgcgcgaac ctggttggca cacgcaatgt ggcctaccct
    17881 ccgtggctcg ggcgtgcggg tttcaccgtg agcgtgccgt ccgcaccgca agtaccacta
    17941 gaggtgggag ctcaaagtct agtcgtcggg ccccggcgtg cggccgacac cagcatcgta
    18001 gaagcgggac gtagcttcag cttctccagc tacgtgatgc tggacgtatc catcgcgacg
    18061 cgagagatat atctgcttcc ggggcacgaa tcgcgtttcg cccttcgttc gcgcaacctg
    18121 ttgctgacgc gcgggcccga tccaggcttc tctggattcg agtacccggt gacgccggcc
    18181 gagatcttcc tagaactctc ccatacgtac tgaagtcgac cgccgcacgc gaggcgcgcc
    18241 gcaggtcggc gacaacggcc agctctcgac ggtttgcgcc agcggctccc tcaccaatcg
    18301 ggtgggctcg gttcagctct ttggcgggct gcggtgggct cgggctggga ctacactccg
    18361 cgcccatgga tttctaccaa gtcacgattc cccatacgat caagctgctc gccaagctgg
    18421 agacctggct ggacaaggcc gcggcgttgg cagaccagaa gaaattcaaa gccgacacgt
    18481 tgctgcaagc tcggctggcg cccgaccaat acccgttggt gcgtcagatc caggccgcct
    18541 gtgatcaggc caagttcagc gccgccaagc tgaccggcaa gcagccgccg tcacacccgg
    18601 acacggagag cacgtttgcg gagatccggg cccgaattca atcggtgcgc gagtacttgg
    18661 ggacgttcaa gccagaagac ttcgcgggcg ccgcggagcg gccctgtagt cacgtttgga
    18721 tgcaaggcaa agccctgcgt gggcgcgact actggctcga aatggggcaa cccaacttct
    18781 tcttccacta caccacggcc tacgccatcc ttcgccataa cggcgtcgat ctcggtaagt
    18841 ccgactatct cactgatctc tccttcatga actgaagttt cccgcagtgc ggaaccgcgg
    18901 cgcgaccccg acttcacggt cctgacgagt aaactctaac tagtaggctc gaagaacgcc
    18961 gagccgaaac gtcggaagtc ggggcgtcga gccgaagctc gtcgcagaga atcgcgccga
    19021 gcaacccgaa gatcaatctg cagggtagcg gctcgtgagc gggaagccgc taggcgggcc
    19081 ggcagtttgc ggtatgctcg cggccgtgaa cgacctccct gccccggaaa acccccctat
    19141 cgtagctgct gtgcagcgag cgcgcagcgg tttgcgccgt gatgtgcaag cgctgctcga
    19201 gcagttggac gatgccgagt tgctcgtccc gctcgcgcgc gaccttccgg atgcgccgga
    19261 aggggagcgc atcgagctcg agggcgagtt gtcactggtt ccacacctgc tcccggatac
    19321 ggaagggcag ttgtttgctc ctttgttcac ccacgccgag ccggtcgagc ccgtggcatc
    19381 cactctcgag tggacgaccg acggtgaagc gctgaagctc tgcgcctttc cggctcgtat
    19441 tgcgctcgaa atggcgctgg aggtcatcga caatggtcac gtgctggggc tcgtgatcga
    19501 ccccggcgcc gagagcgagc tctgtttgac gcgcgcggag gtgagcagcc tgctacttgg
    19561 tcgggccgtg ccgttactgg cctacgttca aaacatcccg gaggaagaac ggggcagcac
    19621 cttggtggcg gagggagccg aaccgccgcc cccggaatta cgctccgcgc tcgacgtctg
    19681 gctcgccgca acacccgagg tacgaaccca tcgcttggag cgcacgttca acccggatcg
    19741 cgatctcgag ccgcatctga ccctcacgct gcgcgtgcct gagtcggcga accgaagcag
    19801 ccttttcgaa ggtgtgacgg gggcattgag cgggacggtg ccgccgcccg gctacttgga
    19861 cgtcattttc gagccatgcg agagctgagc acgagcccga gcgacgtcgg gcaactccag
    19921 ctgatggaag gtgcgcgccc ggacctagcg acggacggcg cctcgcggtt gtcttggctc
    19981 gagcgcgcgc aaatccgctt cgtgcggcgc accttcgatc ccgggccggt ggatcgctgc
    20041 gtgcgtgctt gccagcgaca catcggatcc acctggattc attactgtac gcgccacctt
    20101 cgcagtgtac acggcttgga tcggttgcca gagttgcggc ccgagcagag cttcgtgttg
    20161 gtcagcaatc accgaagctt tttcgatttg tacgtcgtga tgggcgacct ggtgcgacgt
    20221 ggtttgcctc accgcatcgt gttcccggtg cgctccaagt tcttttatac cagccctgcg
    20281 gggttgttcg tgaacggggt aatgagcttc ttcgccatgt acccgccaat ctttcgcgag
    20341 cgtcgtcagg cggcgttgaa ccccaagagc ttggatgaat tgatttggtt acttcgtcgc
    20401 ggtggcgtgt tcgcaggtct gcatccggag ggcatgcgca agctagacga tgacccctac
    20461 accttgctgc cagcgcagcg cggagtgggc cgcatcatac agggggcgcg cgtgccggtt
    20521 attccggtgt tcatcaacgg gctcgagaat gatttgccgc gtcagatcac cggcaacttc
    20581 aatgctacgg ggcgaccgat cctggtcgtc ttcggcgagc cgatcgattt ccgcggtttg
    20641 ctcgagaagc cgagctccca gcgagtgcat caggccatcg cagaccgcac tctcgaggtg
    20701 attgccgggc ttggacgtga agagcgtgag ctccgtagcc ttctaggcgc cgagccggca
    20761 tccctcacga gccccgcgcg cggttgaact tcgggcgcct cgcccgctac tgactgcggg
    20821 gcgcctgcaa cacgatgcct gcggaaatcc agagggaagg tcgtccaagt tcggcgatct
    20881 ggcggcccgc cacgtgcacg ctgggcgagt caatcgccgc gctcgtgtcg agtaacaggt
    20941 acgcgccaaa ggagggagta aaccagccag ctccgccgac ggcagccgcg acgacggggc
    21001 tcgtatgcgt ccgagagacc ccaacgtagc caggctctgc tcgccctcgg atcgtatact
    21061 gagccacccc cgccccggcc aggaaccaca gctcgatccc tccgtcgagt gcgatcgtgc
    21121 ctccgccgcg cgccgtcccc cagcgtgagc cgacatcgac tttaccgacg agatcacgga
    21181 tatcgccagg gaccaacgaa ctggctgcaa aaactcctgc aacgaagtgc gagcgcccga
    21241 acagcaacgc accctgcgct ccgagcccgg tcatgccgga gctcggttcg aagtagactg
    21301 cgggcgccgc gagtaagctc aaaccgttgc cgctgcgtgg cgcgagcgag caggggtcag
    21361 cccgcttctt cggagctaac acggaaggta ctcgcgctgg ctttggaagg gtctcgtccg
    21421 gttgcttcac cgcgcgctcg ggcgtgggag agggttccga cgggtgaccg agcaaggctg
    21481 cgcgcaatgc ttcgaccgtc ctcacgccca cctcctctgc ggtgagatcg cggcgactta
    21541 gatcgaactc ttggacgatg ggggtcgccg cgtacggtcc ataggcttgg atccgaactc
    21601 ggttcgagat gacctcgacc accagcaaac ccgcttcgct ttggcgcgcg tcgggctcgg
    21661 gggggctcgg ggaggacgca gtcgagatcg tcgcatcgcg ccccatcgcg gagagctcgc
    21721 cgcggactcg gatcagagcc tcgcgcaaca ttggacttgg ttgttccggc acgcgaatca
    21781 gcacgctgcg ggtgctgatg ccgttcgact cgactccaac gctcgcgggc gaggcgggct
    21841 gggctgctgc gtattgcgta accgagaccg caagcaggac cgcaatcgct gcgcgtgttg
    21901 ttccgcgccg tattggtcca cacggtgttg gtccacacgg tgccgcgcga agccggcctt
    21961 gccccatctc aaggttgaga gagcaaagcg cgtgcgttcg atgcgtacgc gccgttcggg
    22021 aaacgctcca agtattcacg agcggctttt tgcgcgacca atggtccttc gagcttctgt
    22081 accagcaaca atctacgccc ggcggcctcc gggacgtagc tgtctttgcg agcctcggct
    22141 aggtacgtat ctagccaacc caaggcttcg ttcaaacgac cttgttgctc ctgaatacga
    22201 cccaggaaga aggcagcctg gcgagcggtc cggtgagccg cgaagcgctt gcggagggct
    22261 gtccacgctt gcacggcgag ctgcgagcgc ccggtatagt tggcggcgtg gcctagagcg
    22321 ttgagctccg aggcactctt ctcccgcagc acctgctcca gaccttgact ctcggctgca
    22381 cttaccacct ccgcgaagcg tcctttggcg accaagctcg cgaaatccag gtcttccggg
    22441 gcggccactt cgctgcgctc attgcgctgt tcgccgcgtt cgttacgggc ttcgttgcgc
    22501 tcacttcgag ccgcgattcg cgctcggtcc gggtcgttct tggcgctgcg cccggaatca
    22561 ccttgcaagc gtcccggcac cgacacgacc ggtgcttgca gatcgaacga gctgcctgcc
    22621 accgcgcttt gggctgcgct gagctccagc ttttgaccgc tcggcactgt gcgatttagg
    22681 ccgtccgggc cgagcacccg cacgttatcc ccatgcgaga gcaaggagaa gttctcttgg
    22741 acaggatccc agctcacctc gaaacgggcc gcttgcgcct gaacttcgta gggcccgatg
    22801 aaaaagcggt actcagagtc cgaacgggga tgaaggtcta cttcgagccg accacgttcc
    22861 aaccgtgtgc gcgaagcgtg cgcgcccaag acctccaccg agagcgaggt atgaggcgcg
    22921 acgatgagtc gcgtgccatc tccgaaatcc acgttcttgg aggccgcctc ggtgtgaatc
    22981 gccccgctcg gcgatgccgc tagcgtcgcg ttctcggacg gcgtgctgaa caacccccgc
    23041 gcgccgaatg ccgcggcggc cagcgctgct accgccaccg ccgggaccgc aaaccgaccg
    23101 aacacgcgcg ccagcggtgt tcgcaccggg taacggaatg cagggacgcg ccgttcgtcc
    23161 tgatcttcac gcgcatgaat ctgtatcaaa cgccatgcgc gttcccgatc gtccgcttct
    23221 cgatcttcgg cgtgttcttc ggccaacgcc cgaaacgcgg cgttcacgtc ttcgggttgc
    23281 agcgcgcgcg acggccgcga gggttgattc gggttagaca cggccgcgcc tcgtacgctc
    23341 ctggacgtaa tctgcgagca aagcgttgcg gctgactcgc ttcaggacgt gctcgtttgc
    23401 ccgacgcacc cggcgtttcg cggtcgacag cgacacatcg agggccgccg cgatctcggc
    23461 gatttgcatg ccctccagac tgtgcagcac gaaagccatt cgatcgttca cgcgcaactc
    23521 gtccaagatt cggctcagct gaatcagcgc ggccttggct ttcggatcgt cccggccggg
    23581 ttcactctcg agctccgggc tcaatccgga cagtttcagg aacagccggc gtcgtttgcg
    23641 acgccgcacc tcgcggcgga tcgcgttcat cgtgatcgac acgaggtagc tgcgtaccgc
    23701 ggtcggcgtt cggatacggt gcgcgctctc gaagaaggtc acgaatactt cgctgaccag
    23761 atccgaagct tccgcgttgg gtcccaacga cttcaaaacc aagccgtgca ccagcccgtg
    23821 gaattcatcg aacgcgcgac gctgccagtc tggtgaattc cccgaaatcg cggaatggag
    23881 gtcctccgag ctacgaggtg gtagcaggga tttcgaggag accaacggcg gcaccaagac
    23941 ccggatgcta gcagttttcg acaccgatgt ggccgacgag ttaccctggt gacgctaggc
    24001 cagttggcga tagcaccgcc ggagatcgac gataacccgc tgaaaaaaaa ccacaaacaa
    24061 aatgacctga tttggggatg aggcgcgcgc ggaatcatgc actgtggctg cgccctccgt
    24121 atcgattgcg cgcagcgcct ctcccggggt tcaaagcagc gtgcgccgtg cgcggcgcag
    24181 atggccagtg ttccaggttg cgaatgcgtg ttgtttgggt acatctgttc cagtgagatg
    24241 agtccggtgc gatgagaatc gagctccaca ggctggcgtg gatggcgagt tccgttctcg
    24301 tcctggcgct gggctgcaat tccacccgag tcgacgcgat cctcgccctc ggcgagaagc
    24361 cgattcaaga ctgtactgag acgcgcggta cgctgaaggc cggcacctat cgtgtgcgct
    24421 ctgtcgatcg gtcgcgctgc tggcgcatcg ggcaagagac ctccattttt ggaaacctgg
    24481 gctatctgat cgcggagact ccggaatgta ctctcgaggg tgagctgtgg tcgttcgaga
    24541 actccgattc agtgccgggc gccttcgaag tacacagcac cagtcactcg ggcttcagcc
    24601 tggatgttca ggagtccagc aaggccgatg gcacgaatgt cattacttac ggttctcaca
    24661 cgttgcagaa tcagaggttc tacgtgcgtg agatcgagcc gggcgtttac gagttctcgc
    24721 cggcacacgt aatgaccagc tgtatatccg agcggaactc gacgagcatc ggtgaatcgg
    24781 cgattcagat cgagggctgc gtgcctacgg cctctgcgca acattggcag attttgagcg
    24841 atggctgcgg tggtgcttcg ggctcgtgac cgccgcaccg gaaatgattg acggaagcga
    24901 atgaaccggc gccggctgcg ccggtgtgac tacaaatcgg agcgctccgt gatcgatcgt
    24961 gacagattcg aggcaccgtc gttactacgc tcaatacttg ggcctgatca gcagccctac
    25021 cgaggcccca ctgcccagca gcagaacgac tgccgagcag cagaacgact gccgaaacac
    25081 gggttgcatc accacccgtg tttagaacga aagctgcttt atcgagttcg ctcgtgaccg
    25141 gttcagttcg cgcgttcgaa gcagggatat gggccgagct atagacatga ccgagtcagg
    25201 gacattggca ttgaggttca acgcatgaag cgcacctgtt ctctttctct gatgggttgg
    25261 gtgggaacgc tcgtgcttgg ctgcacgggg acggtttccg atccgggcgc cggtgccacc
    25321 ggtggcgggg cgggcacccc gaacgcgggg caaccgaacg ccggggcgac ccacacgacg
    25381 gtcaatccgg atggctccac caccacgacg aagcccgacg gaaccaccgt aactacgatg
    25441 ccggacggca gcacggtgac catgaatccg gacggcacca cgacgacgac tccgcccggc
    25501 acgggctctg gaggcacacc tggtacgggc tccatcgaac caggtgacgt gacccagccc
    25561 gcgactccgt tcgaagccgt gacggcgctg acttcggttc gcaaggttaa gaatttgatg
    25621 accggtttgg ccccgacgga cgccgaggtg caggcggtcg cagaacgtgg tgccgccggt
    25681 ctgcaggagc tgatcggcaa gtggatgaac gaggccgata ctcagcccaa atttcgcgac
    25741 aagatgctgc agttcttccg caacgccttt caacagacag gcttcatccc cacggaggat
    25801 ttcaagcatc agttgctcga aaatggtggc ttcgactttg gaccgctcgg cactggcgcg
    25861 gtgggagacg atatctattt ccggctagtg cagaacattc aagacagctt cgcgatgacg
    25921 gcgtggcagt tcgtcgtgga tgggcgacca ttcagcgaag tgctcacgac cgatcgcttc
    25981 atgatgacga cggcgctcaa ggccgtctac gtccagatcg agatgcccaa tgatcagccg
    26041 tatgcgagct tcggccagtc tgcgtcgaac ttcgtcaagt ggtcgatcaa tatggcggcg
    26101 gacgcggtga tcccaatcga gaccgtggtc ggcaatcgtg cggccctgca attcgatgat
    26161 caaaaaccag ccgggggtgg ctctggattc agcatcggca acacgtgtca gggtaccgat
    26221 gtcgtcaagg aatactctgg ctatgcgcag ctgtttcaac gtctgatcgg ctacacacca
    26281 cgcaacaagt tcgtcgcgaa cccagagtgt tgggagcacg cctcgaagcc atacttccag
    26341 gtgacggaca cccaagattg gcagtgggta aagatcaaga agatggccga cggcgagtcg
    26401 tacatttacc cgtacgacat ccctgcgctg cgcaccgcga gcgagctctc tctcaaaatg
    26461 ccgcgcgttg gcttcttcac gacgcccgcg ttcctcgctc tttggaatac caacgatagc
    26521 aatcagcacc gggtcacggc gaatcaaacc ctgttggtcg ccctcggcgt ttcatatacg
    26581 ccggaggcgg cgttcacgcc ggcgagcgtg gagggcttgg ccgtgctcga caagaaccac
    26641 tccgtcgaag ggtccgaatg ctacgcatgt catcgtggcc tggatccgct
//
LOCUS       DASTKU010001103         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_441157, whole genome shotgun sequence.
ACCESSION   DASTKU010001103 DASTKU010000000
VERSION     DASTKU010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441157"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VFQ61_39480"
     CDS             complement(<1..863)
                     /locus_tag="VFQ61_39480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960639.1"
                     /translation="MNPLFDPYFGWLVNKAGDVAISSFGLYRLLFAPFVSAGFRDWTS
                     RRRACAVALQARTRVPAYRAFLEQHAALRVRHFSELPVMDKANYIQRWTLEELCLGGE
                     LPKHGAVIDESSGSTGTATNWIRGPGERFATRRLIQYSARATFGSESFVLLNAFALGP
                     WATGMNVSMSLVDRCILKSIGPDIKKVIASLEQLGPKHRYVITGYPPFLKTLVDTADI
                     DWARYDICAVVGGEGMSEPLRAALNRCFRKTISSFGASDLEINMATETDSTIGLRREL
                     QSNSRLARDLYG"
     gene            complement(871..1743)
                     /locus_tag="VFQ61_39485"
     CDS             complement(871..1743)
                     /locus_tag="VFQ61_39485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960640.1"
                     /translation="MTERMLPEEDHGNLYVASSFGIFAATLERAAAHFGWKLEVQRCE
                     ELEVSELHRAGPRVRLGQARIVGAMPPEPHAPLFEARRTSRLPYHDRPIAPEALASFQ
                     KTVEAAGQRLLHYDAPDVVQPLLLLNAQAIIDNLQLEREREEIRGWYRLGRTPEYGDG
                     LFRAPMNQPAWELWAAFAMPSLFRREPFKSFAIRRYLATQRGTRHVALLCGRFREWPD
                     LFVAGRMLFELWLELARFDVYMQPMGSMLTNPAYSAEIARRFGRDDCWLVLRLGYSEP
                     PPRAPRLENILLDA"
BASE COUNT          421 a          663 c          677 g          366 t
ORIGIN      
        1 ccgtacaagt cccgtgccag gcgcgaattc gactgcagct ctctccgtag accaatcgtc
       61 gaatcggtct cggtggccat attgatctcg agatcggagg cgccgaagct cgaaatggtc
      121 ttgcgaaagc agcggttgag cgcggcgcgc agcggctcgg acatgccctc tccgcccacc
      181 accgcgcaga tatcgtagcg cgcccagtcg atgtctgcag tatcgactag cgtcttcagg
      241 aacggcgggt atcccgtaat cacgtagcgg tgcttcgggc caagctgttc caggctcgcg
      301 atcaccttct tgatgtccgg accaatggac ttgagaatgc agcggtctac gagcgacatc
      361 gaaacgttca tgccggtggc ccaggggccc agcgcgaagg cattgagtaa cacgaaactc
      421 tccgacccaa aggtcgcgcg ggccgagtac tgaatcaggc gccgagttgc gaagcgttca
      481 ccggggccgc ggatccaatt cgtggccgta ccggtggacc cggaggattc atcgataacg
      541 gcgccatgct tcggcagctc accgcccagg cacagctctt cgagggtcca gcgctggatg
      601 taattggctt tatccatcac cgggagttcc gagaagtggc gcactcgtag cgcggcgtgc
      661 tgttcgagga acgcgcgata cgccggcact ctcgtccggg cctgcagcgc gacggcgcag
      721 gcgcgccgcc gactcgtcca gtcccggaac cccgccgata cgaaaggggc gaacagcaaa
      781 cgatagaggc caaatgacga gatcgcgaca tcaccggctt tgttgacgag ccaaccgaag
      841 tacggatcga aaagcgggtt catgcgcggc tcaggcatcc agtagaatgt tttctagtcg
      901 cggggcccgc ggcggaggtt cggaatagcc caggcgcagc accaaccagc aatcgtcacg
      961 accgaagcga cgggcgatct cggccgagta agctgggttg gtgagcatcg aacccatcgg
     1021 ctgcatatac acatcgaatc gggcgagttc gagccagagc tcgaacaaca tgcgcccggc
     1081 cacgaacaaa tccggccatt cgcgaaagcg cccgcacagc agcgcgacgt gccgagttcc
     1141 gcgttgcgtg gcgagatagc gccgaatggc aaagctcttg aacggttcgc gcctgaacag
     1201 gctcggcatc gcgaacgcgg cccagagctc ccatgcgggt tggttcatag gagctcgaaa
     1261 caagccatcc ccgtactcgg gagtccgccc gaggcgatac cagccccgga tctcctcgcg
     1321 ctcgcgctcc agctgtaagt tgtcgatgat ggcctgtgcg ttcaagagca gtaggggctg
     1381 caccacgtcc ggcgcgtcat agtgtagaag acgctgaccc gcggcctcca cggttttttg
     1441 aaacgacgcg agcgcttcgg gggcgatcgg gcggtcgtgg taaggtagcc gggaggtgcg
     1501 ccgagcttcg aacagcggtg cgtgcggttc gggcggcatg gctccgacga tgcgcgcctg
     1561 acccagccgc acgcggggac ccgctcgatg caactctgaa acctcgagct cttcacagcg
     1621 ctgcacttcg agcttccaac cgaagtgcgc ggccgcgcgc tcgagcgtag ctgcgaatat
     1681 tccgaacgag ctggccacgt agagatttcc gtggtcctcc tctggtagca tccgttccgt
     1741 catcagccaa agctcggcac aatcggagcc cgcggcacgg gggcgaatcc ggaagggttg
     1801 tgtgttgtgc ggtgtagggg ccaaccgcgc gagtttagag atatcttccc aagaccacat
     1861 cccgatgcgc ccagcttaga gcgagcatgc tgcacacgct agcgcacgat gcgcatttac
     1921 cacgcgggcg cggagaatcg gcccgcgttt cgcgcgggga ccggcgggtg cagggcttgt
     1981 catgctcctg aattgggacg atgctgcctg gcattcatga aaaccaagcc gacccctcgg
     2041 ccctcgctct cggactttcc gccccgtccg gtcttgaagg gccgaagcgt ggaggttcgc
     2101 accctatcca agctcgtgaa gggcggc
//
LOCUS       DASTKU010001104        10325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_888435, whole genome shotgun sequence.
ACCESSION   DASTKU010001104 DASTKU010000000
VERSION     DASTKU010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888435"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="VFQ61_39490"
     CDS             <1..1534
                     /locus_tag="VFQ61_39490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960641.1"
                     /translation="PEGKLFRITANGDAQVFFDAEEQHLMSVAVAPDGTVYTGASDKA
                     RLYHVTAPGRSSVVYDFGRTEVRAVAVAANGDVFAIANEIRPGSQIPPRLNKPADAPA
                     GPASTPPNPAKGKGTLYRFSRDGRPDQLLDDGDEHFTSLTIGGDGRPYVGTGIEGRVY
                     TVDDNHNAVLVADTDERQITALWLGTKQSYVAGSDPAVVHEVKGVGGPDAIWTSKVLD
                     AGIRARFGRLEWEATGPIAISTRSGNTKEPDDSWSPWSREFEAPSKVDSPAARFFQVR
                     ARFARDPKAVLTDIDVSFVTDNLRAVVTEVTAKSNERGNDDPIKTSGGPVQRRPDPNL
                     NITWKVENPDKDELRYRLKYRLVGTNTWYDLTRPQEKVTKENYSWDTSDLPEGRYRVK
                     VVASDELSNPPDRVTSDEMESGVLLVDNTPPTVEGLKLVGRRLQAVVLDGVGPVQRVE
                     VSVAGSDEWRPVFPADGIFDEQRETIDVDLGAVLPAGPALVSVRAYDGADNFVIRNVA
                     LR"
     gene            1652..2746
                     /locus_tag="VFQ61_39495"
     CDS             1652..2746
                     /locus_tag="VFQ61_39495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960642.1"
                     /translation="MSQSSFGPWRVAGTALCALIWIQACSSSDMNARPSASVGTTGSG
                     GNGGTNVVTPDTGGMTAEGGMGGYNAACGVQVKGCVPDAPLACADFVPAQTLELSAGG
                     AIGADGQAGNWGQAGWAGQGGEIGDGGAGGTGGNRGEGGAAGGLNAGAAGDSSIGGPS
                     GGAGGVPGTPEYACRVSSGKAGPVAACALSGSGRFDEPCFSGADCAAGLECVRVSAVA
                     RCRPYCCTGESSCNSFPGSYCTDLPLAVQGSGGRGTVSVPVCAQAERCDLSEPFPCPE
                     GRVCQCSAGTACSVVRNDGTTACVTPGVGKTGEECPCSPGHVCSQSAMTCLKLCTIES
                     AADAAFGCGTGKCQASAELPRGYGVCVDLK"
     gene            2918..4705
                     /locus_tag="VFQ61_39500"
     CDS             2918..4705
                     /locus_tag="VFQ61_39500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9960643.1"
                     /translation="MLHPEVRNEQIRTLFRQSPSVLIANVVNSAIVATVLWRRELGTA
                     LVLWFTSMLIMAGVRAVHRARYLKSPNSAADAARHGRRFVAGSVTAGALWGGAGWIFY
                     DPQNPLSQFLLLFVIGGMAAAAAGTLCVHLPAFFGFALPALLPVAGRMLIVGDPTHLA
                     TSLIVVGYIGGLSYVANTSQRTFVEAFRLRFENEALLRQLSEAQVHLEQANLTLEQRI
                     AERTRTLEQQGLALREAQRLEWVGKVAGGVAHDFNNLLSVVLGNASFLASEFELPERA
                     RAALKEIGQAATRGAELVRQLLAASQKQHFVLRVFDLNERVLSFGPLLQRFAGERIRL
                     KLELADGPLPVRADVVQIEQVLMNLVANAKDAMVEGGLVTVQTALDADHVVLRVRDTG
                     TGMDTVTLARAFEPFFTTKPAGRGTGLGLSTVRGIVEQTGGQIHVTSELGRGTCCEVR
                     LPRASGPVEDVSPARTPAPTQSAPRTILLAEDEKPVRSVLERLLRREGHRVLVAEDGQ
                     RALDVARSHSGPIHLLVTDVTMPRLNGPELARALRAERAELGVLFISGYSFDQSLPPS
                     DPEARIAHLPKPFDPDTLLREVAVLTRAS"
     gene            4854..5240
                     /locus_tag="VFQ61_39505"
     CDS             4854..5240
                     /locus_tag="VFQ61_39505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET9960644.1"
                     /translation="MSRGRGFINVTERVAQAVADSGVDCGLCHVFIQHTSASLVIQEN
                     ADPSVLRDLERFFEELVPEARAWEHDDEGPDDMPSHVRSALTKTSETIPVDNGRLSLG
                     IWQGLYVWEHRARPHSRRLLVTVMGT"
     gene            5322..5900
                     /locus_tag="VFQ61_39510"
     CDS             5322..5900
                     /locus_tag="VFQ61_39510"
                     /inference="COORDINATES: protein motif:HMM:NF017939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HET9960645.1"
                     /translation="MLDLRTVTPQAWLSCILSDFDAFLLDHAACERKASATGMSFVVR
                     YPDRTALIEPMIEFAREELEHFHIVYRLVAERGLKLTDDYRDPYVNGLRAQCTTSGEG
                     LLLDRLLVAGVVEARGCERLFMVAEALRERDARLHEIYLELARAESRHHGLFLRLARG
                     LFDEARVHERASFFLDFEADLISRLPHRPAVH"
     gene            5990..7333
                     /locus_tag="VFQ61_39515"
     CDS             5990..7333
                     /locus_tag="VFQ61_39515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960646.1"
                     /translation="MRTLDKYLERHAEAGLSELAEQLGRYHAALVIPALGENPELLRG
                     IASACALASGRVLVVLVLNATPKSAPELREQNDLCFQVWSRGAVQLGPNAYLRPEADF
                     DLLLLDRSSPGFELPLKEGVGLARKYGGDVVCALARLGRLELPIVLYTDGDVRLPEDY
                     FARAARAWRETAGNQAPSAWLFPFTHVPAEDGRVWRATRVYESTLRYHVLGLVRASSP
                     YAYQSLGSTLAIPVDAYAQVRGIPKREAGEDFYVLDKLAKLAPLHRLLGAPIEIRSRR
                     SARVPFGTGSNVTRLSSQDGPALVASPDAFEALGCVIRALDRFARGPTARELEAGLAT
                     APAAWQGAIAHAVAQSGLVPACLDAAAKVGAGDLARRLHTWFDALKTLRFLHHLRDAG
                     LPERTLAEALGAARGWLGATDPEDPCAGLELAERRLPPEVGPTLPRYVTHVTRAL"
     gene            7453..9447
                     /locus_tag="VFQ61_39520"
     CDS             7453..9447
                     /locus_tag="VFQ61_39520"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HET9960647.1"
                     /translation="MTPPPAATASGKPVQATTLEAVGLDAKAMDRTANPCTDFYQFAC
                     GTWLAQTPIPADEAVWSRSFSEIRKRNELELKRILEEAAVAGENQPTTQKIGAFYAAC
                     MDEARVDAALEKPIQPLLEKVRKIRDRKELARVVAELHRVGIWPFFDLSPVQDSQDAT
                     HWIANLDQGGLGLPDRDYYLRTDPDSVKLLEKYREHVERMLVLAKVPAAQAKVTAGDV
                     IAIETELAQVSKTRVERRDARAMFNRWKKPALLTASPAFEWNSYLTGVGVPAFEELNV
                     TAPKFVEGVQAVLTKHTPAQLRGYLEWHIVHATAPLLAKPFVDENFRFEQVLTGQPEL
                     RSRFRRCVAATDRALGDLLAQAYVERNFSGESKPAAERMVFAIGAQFKTALERLTWMD
                     AATRGRAREKLERMAYLIGYPKVWKSYDFPIDRARYAESALAARAFDLKRELNKVGQP
                     VDRDEWQMTAPTVNAYYDPQRNHMVFPAGILQPPFYNVGFAVPVNLGAIGMVVGHELT
                     HGFDDEGSQYDAQGNLTNWWEPAVGERFKQKTSCVSKQYDAYEVLPGLHVNGALTLGE
                     NIADLGGVKLAFHAYQELQKNASVLKVADGFSEDQQFFLAYGQAWCARYRPELQRLLV
                     QTDPHSPPRFRVNGPLTNLPEFAQAFSCKPAQPVSQCEVW"
     gene            complement(9705..>10325)
                     /locus_tag="VFQ61_39525"
     CDS             complement(9705..>10325)
                     /locus_tag="VFQ61_39525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HET9960648.1"
                     /translation="CDPQTGECVSNKLNTGECNDGSACTIDDSCQDGVCQGSAKECPT
                     VQCFAAGKCDAESGNCSYSPLRDGASCENDTGTCSKGTCVPNPIIDNTAGAGGEGGVG
                     PVPVEGGSGNQGGATNNGGESTAAGESALGGTTSGTVTSTGGRSSGGSTTSEPTGDRG
                     EPFKRDPGGCSCSVPGPESNSRTAPVALSLSGLLATLIAVRRRRRS"
BASE COUNT         1769 a         3155 c         3434 g         1967 t
ORIGIN      
        1 gcctgaaggc aagctgttcc ggattacggc caatggcgat gcccaggtgt ttttcgacgc
       61 cgaagagcaa cacctgatga gtgtggccgt ggccccggac ggaaccgtgt ataccggcgc
      121 cagcgacaag gcgcgcctct atcacgtcac cgctcccggg cgtagcagcg tggtttacga
      181 tttcgggcgc accgaagtgc gcgcggtagc ggttgccgcc aacggcgacg tgttcgcaat
      241 cgcgaacgaa attcgacctg gatcgcagat tcctccgcgg ctcaataaac cagcggacgc
      301 gcccgcgggc cccgcgtcga cgccgccaaa tcccgccaaa ggcaagggaa cactgtatcg
      361 cttctcgcgc gatggacgac cggaccagtt gctcgatgac ggcgacgagc acttcaccag
      421 tttgacgatt ggtggggatg gtcgaccata cgtgggcacc ggcatcgaag gccgcgttta
      481 tacggtcgat gacaaccaca atgccgtatt ggtcgcggac accgacgagc ggcaaatcac
      541 cgcgctttgg ctcggaacca agcaatccta cgtcgcgggg agcgatcccg ccgtagtcca
      601 cgaagtcaaa ggcgtaggcg gccccgacgc gatctggacg agcaaggtgc tcgacgccgg
      661 aattcgcgcc cggttcgggc gcctggagtg ggaggcgacc ggacccatcg cgatttcgac
      721 gcgcagcggg aacaccaagg agcccgatga ttcctggagc ccttggagtc gcgagttcga
      781 agctcccagc aaggtagata gccctgcggc gcggttcttc caggtgcgtg ctcgcttcgc
      841 gcgcgacccc aaggccgtgt tgaccgacat cgatgtctcg ttcgtcacgg acaacctgcg
      901 cgcggtggtg accgaagtca ccgcgaagag caacgaacgc ggtaacgacg atccgatcaa
      961 gacctccggc ggtcccgtcc agcgtcggcc cgatccgaac ttgaacatca cctggaaggt
     1021 agagaacccg gacaaggacg aactccggta ccgactcaag taccgcttgg tcggcaccaa
     1081 tacttggtac gatctcacgc gcccccaaga gaaagtcacc aaggagaact acagctggga
     1141 cacgagtgat ctgcccgagg ggcgctatcg agtgaaagtc gtggcctcgg acgaactctc
     1201 gaacccgccg gaccgggtga cgagcgatga gatggagagc ggagtgctct tggtggacaa
     1261 cacgccgccg accgtagaag gcttgaagct cgtggggcgg cgcttgcagg cggtggtgtt
     1321 ggacggagtg ggtccagtgc aacgtgtcga agtgagtgtt gccggcagcg atgagtggcg
     1381 tcccgttttc ccggcggacg gcatcttcga tgagcaacgc gagacgatcg acgtcgacct
     1441 cggcgcggtt cttccggcgg gtcccgcgct cgtcagcgtg cgggcttacg acggtgcgga
     1501 caacttcgtc attcgcaacg tcgcgctgcg ttgaacggcg gcgctcgagt ccaggctcgc
     1561 ctcgggcttg accgatggcg ccgcacaact ccgggtgcat cgtgccaatc ctcgacgccg
     1621 ggcttttgcg ctgtagatta agctcattct tatgagccag tcttccttcg gcccctggcg
     1681 cgtggcgggc accgccttat gtgccctgat ctggattcag gcttgttcgt cgagcgacat
     1741 gaacgctcgc ccctcggcaa gtgtggggac gaccggctcc ggcggtaacg gtggtacgaa
     1801 cgtcgtgacc ccggataccg gcggcatgac tgcggaaggc ggtatgggtg gctacaacgc
     1861 ggcgtgcggc gtgcaggtga aggggtgtgt gccggacgcg ccgctggctt gtgcggactt
     1921 cgttccagcg cagacgctcg agctgagcgc tggcggtgcg atcggcgcag acggtcaggc
     1981 tggcaattgg ggccaagcgg gatgggcggg ccaaggcggg gaaattggcg acggtggggc
     2041 aggcggaact ggcggcaatc gtggtgaagg tggggccgcg ggtgggctca atgccggagc
     2101 cgccggggat tcctcgattg gaggcccgag tggtggcgca ggcggtgtgc ctggtacgcc
     2161 ggagtacgcg tgccgggtga gcagtggaaa ggccggtccg gtcgcagctt gtgccctgag
     2221 tggcagcggc aggttcgatg aaccttgttt ttcgggagca gattgcgcgg cgggtctcga
     2281 gtgtgtgcga gtatccgcgg tggcgcgttg ccggccctat tgctgcaccg gggagagttc
     2341 ttgtaactcg tttcctggca gttactgcac ggacttgccg ctcgcggtcc aaggttccgg
     2401 cggtcgaggc acggtttcgg ttccggtttg tgctcaagcc gagcggtgtg acttgtccga
     2461 accgtttccg tgtcctgagg gaagagtttg tcagtgctcg gcgggtactg cttgtagcgt
     2521 cgttcgaaac gacggcacca ccgcgtgcgt gacacccggg gtcggcaaga cgggtgagga
     2581 atgcccgtgc agcccaggac acgtatgttc tcagtcggcc atgacatgcc tcaagctctg
     2641 cacgatcgag agcgccgccg atgcggcctt tgggtgcggt acgggtaagt gccaggcgtc
     2701 ggctgagttg ccgcgcggtt acggggtctg cgtcgacctg aagtagaacc cggacgggtg
     2761 cgtagccgcg gttctgaatg taagggcgct cgccgtaagc tttctgttgg ccccgctcgc
     2821 ccgttcaccc ggatagttgt ataccggtga gtgggctttc ccgaggggcg agccgcggcg
     2881 tgcaccgcat cacattggga taggctggtc gaagttcatg ttgcatccgg aggtgcgcaa
     2941 cgagcagatc cgtactctgt ttcggcagag cccctccgtg ctcatcgcca acgtggtgaa
     3001 ctccgcgatt gttgcgacgg ttctctggcg gcgcgagctc gggacggcct tggtgctctg
     3061 gttcacctcg atgctgatca tggccggcgt acgcgccgtg caccgggccc gctacttgaa
     3121 atccccgaac tcggctgcag atgccgcacg ccacgggcgc aggttcgtcg ccggctccgt
     3181 tactgcgggg gcgttgtggg gcggagcggg ctggatcttt tacgatcctc aaaacccgct
     3241 ctcgcaattt ctgcttctgt tcgtgatcgg cggaatggcg gcggcggcgg cgggcaccct
     3301 ctgtgttcac ctcccggcgt tcttcggctt cgcgttgccg gcgctactac cggtggcggg
     3361 ccgcatgttg atcgtcggtg atccgacgca cctcgcgacc tcgttgattg tcgtcggtta
     3421 tatcggcggg ctctcgtacg tcgcgaatac gagccagcgc acgttcgtcg aggccttcag
     3481 gttgcgcttc gaaaacgagg cgttgctccg acagttgtcc gaggctcagg tccacctcga
     3541 acaagcgaac ttgaccttgg agcagcgtat cgccgagcgc actcgcacgc tcgagcagca
     3601 agggctcgcg ctgcgtgaag cgcaacggtt agaatgggtg ggcaaggtcg cgggcggggt
     3661 cgcgcatgat ttcaacaatc tgttgagcgt ggtgcttggc aatgcgagct tcttggcttc
     3721 ggaattcgaa ctaccggagc gagcgcgcgc cgcgctgaag gagattggtc aagcggcgac
     3781 gcgtggcgcg gagctggttc gtcaattgct tgcggcgagc caaaaacaac acttcgtgtt
     3841 gcgggtcttc gatttgaacg aaagagtgct gagcttcgga ccgttgctgc agcgcttcgc
     3901 aggggaacgc attcgcttga agctagagct cgccgatggg cctctccccg tgcgcgcaga
     3961 cgtggttcaa atcgagcagg tgttgatgaa tctggtcgca aacgccaaag atgcgatggt
     4021 ggaaggcggc ctcgtgaccg tgcagaccgc gctcgacgcg gaccacgtcg tgctccgggt
     4081 tcgcgatacg ggcacgggca tggacacggt caccctggcg cgtgccttcg aaccattctt
     4141 caccaccaaa ccggcgggca ggggtactgg gctgggcctc tcgaccgtgc gaggcatcgt
     4201 ggagcaaacg ggaggacaga ttcatgtcac ttccgagctc ggccgaggca catgctgcga
     4261 ggtgcgctta ccgcgcgcga gcggaccggt agaagacgtg agcccggctc ggacgcccgc
     4321 gccgacccag agcgcgccgc gcacgatttt gctggccgaa gacgaaaagc cggtgcgatc
     4381 ggtgctcgag cggctcttac gccgcgaagg acaccgcgtg ttggtggcgg aagatggcca
     4441 gagagcgctc gacgtcgcgc gctcccattc ggggccgatc catttgttgg tcacggacgt
     4501 gaccatgccc cgcttgaacg gcccggaact ggcgcgcgcg ctgcgggcgg agcgggccga
     4561 gctcggggtg ctcttcatct ccggctatag cttcgaccag agcctgcccc cgagcgatcc
     4621 cgaggcaagg atcgctcacc tgcccaagcc cttcgacccc gacacgctgc tgcgtgaagt
     4681 ggccgtcttg acgcgcgcca gctaatccgc gaccgcagcc ctcgaatacg gcctggttgt
     4741 gctctcgttc gtccgtttcg cggcagagcg cgggctttcc gcaaaatagc tggcgagaag
     4801 agacggctcg gcgcatacct cggggcgtgt tgcaccgggt aaccctcgag atcatgagtc
     4861 gcggccgcgg cttcatcaac gtcacggagc gtgtcgcgca agcggtggcg gatagcggcg
     4921 tcgactgcgg cctgtgtcac gtcttcattc agcatacctc cgcgagcctc gtcattcagg
     4981 agaacgcgga cccgagcgtg ctgcgcgatc tcgagcgctt cttcgaggag ctggtgccag
     5041 aagcccgggc ttgggagcac gacgacgagg gccccgacga catgcccagt catgtgcgtt
     5101 cggcactgac caagacctcg gaaacgatcc ccgtggacaa cgggcgcttg agtcttggca
     5161 tatggcaagg gttgtacgtg tgggagcatc gcgcgcgccc gcactcgcgt cgcctactcg
     5221 tcaccgtgat gggtacgtga cttcgctcat ccgagccgcc gcgggccttt ggcgcgcgcg
     5281 agctcgccct gtgttgatag agttcttgga gtagagagaa aatgctggat ctgcgcaccg
     5341 tgaccccgca agcctggcta tcttgcatcc tgtccgactt cgacgctttc ttgctggacc
     5401 acgcggcttg tgagcgcaaa gcgtctgcaa ccggcatgtc tttcgtggtg cgctatccgg
     5461 accgaaccgc gctgatcgag cccatgatcg agttcgcgcg agaggagctg gagcacttcc
     5521 acatcgttta ccggctggtc gcggagcggg gcttgaagct gacggacgac taccgtgacc
     5581 cttacgtgaa cggtttgcgc gcgcagtgca cgacgtccgg agaggggttg ttgctcgatc
     5641 gcttgttggt ggcgggcgta gtcgaggcac gcggctgcga acggctgttc atggtggcgg
     5701 aagcactacg tgagcgtgat gcccgcttgc acgagattta cctcgagctg gcgcgcgccg
     5761 aatctcgtca tcatggcctg ttcttacggc tcgcgcgcgg gttgttcgat gaggcgcgag
     5821 ttcatgaaag ggcgagcttc ttcttggatt tcgaagccga cctaatctcg cgcttaccgc
     5881 atcgccccgc cgtccactga gtttgctcgg agagcggact cgcccgccgc gcacgcaata
     5941 ccggattgga cgagcgcttt ggcattgaag aaacgagagg agtcgaacgg tgcggacgct
     6001 ggataaatac ctcgagcgcc acgcggaagc cggtctctcg gagctcgccg agcagctcgg
     6061 gcgttatcac gcggcgctcg tgattccagc tttgggtgag aacccggagt tgctgcgcgg
     6121 gatcgcctcc gcttgcgctc tggcttcggg ccgagtgctg gtcgttctag tgctcaatgc
     6181 aaccccgaaa agtgcgccag agctgcgtga gcaaaacgac ctctgcttcc aagtgtggtc
     6241 gaggggagcc gttcagttgg gccccaacgc ttaccttcgc ccggaagcgg acttcgacct
     6301 gttgctgctc gatcgatcga gcccgggttt cgagttgccg ttgaaggaag gggtcggttt
     6361 ggcgcgcaag tacggtggag acgtggtttg cgcgctggcg cgcctcggcc ggctcgaatt
     6421 gccgatcgtc ctctacaccg atggagacgt tcgtttgccc gaagactact tcgctcgcgc
     6481 ggcgcgagct tggcgtgaga ccgcgggcaa ccaagctccg agcgcgtggc tctttccgtt
     6541 cacgcacgtg ccggcggagg atgggagggt ttggcgggcc acgcgagtct atgagtccac
     6601 gctgcgttat cacgtactgg gtttggttcg tgcgagttcg ccctacgcgt accaaagcct
     6661 cggcagcact ctagcgatcc cggtcgatgc gtacgctcag gtgcgcggca ttccaaagcg
     6721 cgaggccggt gaagactttt atgtcttgga taagctcgcc aaactcgcgc cactccatcg
     6781 cctgctaggc gcgcccatcg aaatccgttc gcgacgatct gcgcgagtgc cgttcgggac
     6841 gggctcgaat gtcacgcgcc tatcgagcca agacggaccg gcgctcgttg cgtccccgga
     6901 cgcattcgaa gctttgggct gcgtcattcg cgccctagat cgctttgctc gtggcccgac
     6961 cgcgcgggaa ctcgaagcgg ggctcgcgac agctcccgca gcttggcaag gggccattgc
     7021 ccacgcggtc gcgcagtcgg gtttggtgcc ggcttgcctc gatgctgcgg ccaaggtggg
     7081 cgcgggggat ctcgctcgcc gcttgcacac ctggttcgat gccttgaaaa ccctgcgttt
     7141 cttgcatcac ctgcgcgacg ccgggctccc cgaacgcaca cttgccgaag ctctgggggc
     7201 ggctcggggc tggttgggcg ccaccgatcc cgaagacccc tgcgctgggt tggaactcgc
     7261 ggagaggcgc ttgccgccgg aggtgggccc gacattgccg cgatacgtga cgcacgtcac
     7321 ccgggcactg taggctagcg cgcgatgcgt tctcggatgc gaagaaactg gtttcgtttc
     7381 agcagcctca ccttgggctg tcctatttgg gtcgcgtgtg gtgggcaacc ggccgcggtt
     7441 gctccgaatc ccgtgacccc accgcctgcc gccactgctt cgggcaagcc ggtccaggcc
     7501 acgacgctcg aagccgttgg cttggacgcc aaagcgatgg accgaactgc caacccttgc
     7561 accgactttt accaattcgc gtgcggaacg tggctcgctc aaactccaat ccccgcggat
     7621 gaagcggtct ggtctcgctc cttcagtgag atccgtaagc gaaacgagct cgagctcaag
     7681 cgcattctgg aagaagcggc tgtggccggc gagaaccagc ccacgactca gaaaatcggc
     7741 gccttctacg cagcgtgtat ggatgaggcg cgggtcgacg ccgcgctaga gaaaccgatc
     7801 cagcccctgc tcgagaaggt tcgcaagatt cgcgatcgca aggagctcgc gcgcgtcgtg
     7861 gccgagctgc accgggtcgg catttggccg ttcttcgatt tgtcccccgt gcaagactcg
     7921 caagacgcca cccactggat cgccaacttg gatcagggtg ggctcggctt gcccgatcgc
     7981 gactactacc tgcgcacgga tcccgactcc gtgaagctgc tcgagaagta tcgcgagcac
     8041 gtggagcgta tgctggtgct cgccaaggtg ccggcggcac aagccaaggt aacggctggc
     8101 gacgtgatcg cgatcgagac cgagctcgca caggtatcca agactcgggt cgagcggcgt
     8161 gacgcacgtg ccatgttcaa tcgctggaag aagccagcgc ttctgacggc cagccctgcg
     8221 ttcgagtgga acagctactt gaccggcgtt ggcgtgcccg cattcgaaga gctgaacgtg
     8281 accgcgccga agttcgtcga gggcgttcaa gccgtgttga cgaaacacac cccggcgcaa
     8341 ctgcgaggtt acctcgagtg gcacatcgtt cacgccaccg cgcctctgct ggccaagccg
     8401 ttcgtggacg agaatttccg gttcgaacag gtgttgaccg gacaacccga gctgcgctcg
     8461 cgctttcgcc gctgtgtggc cgcgaccgac cgcgcgctcg gagatctgct cgcgcaggcc
     8521 tacgtcgagc gcaatttctc gggcgagagc aagcccgccg ccgaacggat ggtgttcgca
     8581 attggtgcgc agttcaagac ggcgctcgag cgtttgacct ggatggatgc ggccacgcgc
     8641 ggtcgtgctc gggagaagct cgagcgaatg gcgtacttga ttgggtaccc caaggtttgg
     8701 aagagctatg acttcccgat cgaccgtgct cgctacgccg agagcgcgct ggcggcgcgg
     8761 gcgttcgatc tcaaacgcga gctgaacaag gtggggcagc ccgtggatcg cgacgaatgg
     8821 cagatgacgg cgcccaccgt gaatgcctat tacgatcccc agcgcaatca catggtgttc
     8881 ccggctggca ttctgcagcc ccctttttac aacgtcggct ttgcggttcc ggtcaacctc
     8941 ggcgccatcg gtatggtggt tggccatgaa ttgacacacg gcttcgacga tgaaggctct
     9001 cagtacgacg cgcagggcaa tctcaccaac tggtgggagc ccgcggtggg ggagcgcttc
     9061 aaacagaaga ccagctgcgt ttccaagcag tacgacgctt acgaagtttt gcctgggcta
     9121 cacgtgaacg gggccctgac gctcggtgag aacatcgctg atttgggcgg cgtgaagctc
     9181 gcgttccatg cctaccaaga gctgcagaag aacgcctccg tgctcaaagt ggcggatggg
     9241 ttcagtgaag atcagcagtt cttcttggcg tatggtcagg cctggtgcgc tcgttatcgg
     9301 ccggagctcc agcggctctt ggtgcagaca gatccccatt cacctccccg cttccgtgta
     9361 aacggacccc tcacgaacct gcccgagttt gcacaagcgt tctcgtgcaa gccggctcag
     9421 cccgtctcgc agtgtgaagt ctggtgaagt tgcggagaac gagccggacc cggcgcagaa
     9481 cccagcctcg cgcagcgatt ctgaaatttt cccggaccgg gatgttcctg ggaagcgaat
     9541 gcatgcagga atttgcatcc tgcatgcagg gctgcgccgt cctgcagcag ttcccccggc
     9601 tcgccagacc cattgcggat gagcggtacc tgccttcacg accactcggg cccttattta
     9661 agggcatcga gcggtccgtg aaggcttctc cggggcgcct ccgatcagga gcggcgtcga
     9721 cggcggacgg caatcaacgt cgcgagcagt ccgctcaagc ttagcgccac cggggcggtg
     9781 cgagagttcg attctggccc aggaacgctg cacgaacaac cgcctggatc acgtttgaac
     9841 ggctctccgc gatcacccgt tggttccgag gtcgtcgacc caccactcga gcggccaccg
     9901 gtcgaagtca ccgtgccgga ggtcgttcca ccgagcgcgc tctcccccgc cgcggtgctc
     9961 tcgccaccat tgttcgtagc cccaccttgg tttccgctac cgccttctac ggggaccggg
    10021 cccactccgc cctcgccgcc ggctccggca gtgttgtcga tgatggggtt cgggacgcaa
    10081 gtacccttcg aacacgtgcc cgtgtcgttt tcgcacgagg cgccgtcgcg gagcgggctg
    10141 tagctgcagt tgccgctctc cgcgtcacac ttgccggccg cgaagcactg cacggtcggg
    10201 cattctttcg cgctgccttg gcagaccccg tcctggcagg agtcgtcgat ggtgcacgcg
    10261 gagccgtcgt tgcattcacc cgtgttcagc ttgttgctca cgcactcgcc ggtctgcgga
    10321 tcgca
//
LOCUS       DASTKU010001105         6535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1782654, whole genome shotgun sequence.
ACCESSION   DASTKU010001105 DASTKU010000000
VERSION     DASTKU010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782654"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            734..2134
                     /locus_tag="VFQ61_39530"
     CDS             734..2134
                     /locus_tag="VFQ61_39530"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET9960649.1"
                     /translation="MARHDASARGQQPGPRETRARGSAPARHPHGERGRHNDSRANSS
                     QGPRADIRNVEAQHERAAQAARNAPRAFDAEQVAASPFTELGLHADLVQAVLSEGYTV
                     PTPIQQQALPPVLAGRDLLGCAQTGTGKTAAFLLPMMQRLGSVRVPGRVRGLVLTPTR
                     ELAAQIGERANAYARHFGLTHVVIYGGVQQRDQERKLARKPDVLIATPGRLLDLVSQG
                     LLDLGGVQVLVLDEADTMLDMGFIHDVRRIVALLPKQRQNLLFSATMPPAIESLAQNI
                     LVDPARVSVTPKVVAAETVQQAVYFVERTHKRSLLEKVLSAPDVERALVFTRTKHGAD
                     RLAKQLGATGIRARAIHGNKSQNARERALEEFRSGEANVLVATDIAARGIHVSNVSHV
                     VNFDLPNVAENYVHRIGRTGRAGASGRAISFCDQEERAFLVDIERLLRKRIPVAHPAD
                     PSTHHAATGQAATPSA"
     gene            2239..3375
                     /locus_tag="VFQ61_39535"
     CDS             2239..3375
                     /locus_tag="VFQ61_39535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960650.1"
                     /translation="MPVEIRKHPIGVGLSDFLGVADYLFRGDPHYARPLDLDTKDRLS
                     TRNPFFDHAECALFTAHRNGWCVGRCSAQLDRLHLERHGDSTGFFGFFDTVDDPEVAS
                     ELLTAAGNWLRERGMKRIRGPLSLNYNEEVGCLVQGFDAPAMWLTPHHRDYQAGLIER
                     AGFTPFQELHAWRVATDDVPEAARSLHDGLARLPGVSVRHCNMKEFEVDLRTLLELRD
                     DARRDSWAFAPYTDRELKRLSETLKWILIPELTYLVHVEGRPAAAALALPNLNELTRD
                     LAGQLLPLGLPKLLWRLKVSGPKTFRIALLAVRKEFRALPQFEPLGAMLMIKLKEAGR
                     RSGFQLGEWSWTSADDPAPEATLRAAGAATITKKYRLYERALTS"
     gene            3455..4435
                     /locus_tag="VFQ61_39540"
     CDS             3455..4435
                     /locus_tag="VFQ61_39540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET9960651.1"
                     /translation="MNVFVTGASGFLGSHVAEQLSTAGHHVRALVRRTSDTRFLSGLN
                     NVELVEARLDDTASLERALSGCEYVIHAAGLVKARSEQEFFEVNAHATERLFEAATRV
                     PGMRRFVLVSSLAVAGPSEQGAPVPLERTPAPVTAYGRSKLAGERAVLGKKSSLPLTI
                     VRPPAIYGPRDREVLAFFRAIKLGVLPILGAPTNRMSMIYGPDCAAACVAALSRDLPS
                     GTTLFVDDGDVHTFEELVTTSEAALGRRAFLRLPLPRRVIRAAALGSEFYGRVRNQAV
                     MFNRDKCNELFAEWVCDGSATRQLLDWKPEFTFREGVQRTVDWYKAAGWL"
     gene            complement(4509..5702)
                     /locus_tag="VFQ61_39545"
     CDS             complement(4509..5702)
                     /locus_tag="VFQ61_39545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960652.1"
                     /translation="MAKLWECLLAVPAQWYPPNVPARIEIRTVEKTQNLRDFLDVVQT
                     IYADDPNYIRPLDLDVWQRLSLRHPYFQHARRRIFVAYKDGKCVGRVVAHRNELHLAR
                     YKDDLGFFGFLDTIEDPEVMQALMNAVESWHRASGIKRLRGPITMNMAEEVGCLVEGF
                     DYPPMLLMGHHRPYQAGLLEGAGYEKAKDTYAWRYVVADGLTKRVEKAHEEIKNMPEV
                     RFRRVEKKTMDRDIAQMLELYNDAWQDSWGYVPLTPAEAERIGSEFKFFADLELTRIV
                     DIDGVPSAFAYALPNLNEAAAGLNGQLLPFGAAKFLWRLKGRGLDTARLVGLGIAKRL
                     RGVKKYAGLSAFLYAELHYAGVLRNYKWGELSFTLEDNAAINVAIRALGAKIYKRYRV
                     FEKSI"
     gene            5737..>6535
                     /locus_tag="VFQ61_39550"
     CDS             5737..>6535
                     /locus_tag="VFQ61_39550"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HET9960653.1"
                     /translation="MDRLRLANTMLPRVAADYRTVLWAFVLFPGVALAHYVNPGLIGW
                     LLPISIYLGYCAGIFSHNHNHCPTFRSRAMNDFFSIWLSIFYGYPLFGWIPTHNLNHH
                     KFVNKAGDATITWRYTKKHTFLVAITYFFVSAYWQSEPIREYIQKARRSNGRMYRQVV
                     AQAVAVIGAHVGLLGLGIYLWGPARGALVYAFGFAIPAAFALWSMMFTNYIQHVHCDP
                     WSKDNHSRNFVSPWVNWFVFDAGYHSVHHEHAGTHWSKYRALHEARAH"
BASE COUNT         1139 a         2198 c         2035 g         1163 t
ORIGIN      
        1 agtagccagg agatgtcttt cccggcccgg gagagggctc ggagccatct cagcgtttac
       61 tgaggagcct ctgaggtgca gttaggctgc ctgaggatga agcgcgcgtg gggcttggct
      121 gaacgtgttg gccttgccac gtcctcgcga aaacccgcgg taattcgcga gcgccgttca
      181 gctgtccttg ccgcacaacc aagagcactt cgtgaaatct tcgccccggc gtccgttcca
      241 ttctgcctcc aataacagtt ccaattcaga atccagctct cgcgcgaacg cggcaccggc
      301 cgcgcctcat cgcgcttcgc ccgcacgacg cgctttcgag tcgggcgctt ccgagccgcc
      361 ccgagagcgg accgggttcg atcgcgaagc ccgcgccgac tccggcgcct ctttgacacc
      421 gggctcgggc gcaccactca aggctgcgcc acgctccgcc cgcttcttcg agacgaggag
      481 cccttcacgc ggagagcgag gcccccagct gcgtgagcgc aagccctcct ttggcgggga
      541 gcccaagcgc agcgcaagcg aggccaatgg cgatcagcgg aggggcttgg atcgctcgcc
      601 gcggcaacct gctttcggca cgtcctcgca ccgttcatca cccacgggca gcacggcgga
      661 acgcgcccct ggccgtcacc ccgatcagcg gtttgcgcca cggctcgacc gttctcgcca
      721 cagcgaggac agtttggctc gtcacgatgc gtccgcgcga ggccagcagc ccgggccgcg
      781 agagactcgc gcgcgaggct cggcgccagc gcggcatccg cacggtgagc ggggccgtca
      841 caacgattcg cgggcgaact cgagccaagg tccgcgcgcc gacattcgaa acgtcgaggc
      901 gcaacacgag cgagcggcgc aggcagcgcg gaacgcgccc cgcgcgttcg acgcggagca
      961 ggtcgcggcg tcaccgttca ccgagctcgg tttgcacgca gacttggtgc aggcggtgtt
     1021 gagcgagggt tataccgtcc ccacgccgat tcaacagcaa gccttgccgc ccgtgctggc
     1081 agggcgagac ttgttgggct gcgcgcagac gggcaccggc aagacagcgg ctttcttgct
     1141 ccccatgatg cagcgcctcg ggagcgtgcg cgtgcccggg cgagtgcgcg gcctggtcct
     1201 cacgcccacg cgggaactcg ccgcccagat cggtgagcgc gcgaatgctt acgcccgcca
     1261 cttcgggctc acgcacgtgg tgatctacgg cggcgttcag caacgggatc aagagcgcaa
     1321 gctcgcgcgt aaacccgacg tcttgatcgc gacgccggga cggctgctgg acttggtgag
     1381 ccagggtttg ctcgacctcg gcggggttca ggtgttggtg ctggacgagg cggataccat
     1441 gttggacatg ggcttcatcc acgacgtgcg gcggatcgtg gcgctgctgc cgaagcaacg
     1501 ccagaacctg ctgttctcgg cgacgatgcc gcccgcgatc gagtcgctgg cgcagaacat
     1561 cttggtcgac ccggcgcggg tctccgtcac gcccaaggtg gtggcggccg aaaccgtgca
     1621 acaggccgtc tacttcgtgg agcgaaccca caagcgctcg ttgctcgaaa aagtgttgtc
     1681 cgcgcccgac gtcgagcgcg ctctggtatt cacccgcacg aagcacggtg ccgaccgcct
     1741 cgcgaagcaa ctcggcgcga cgggcatccg cgcgcgcgcc atccacggca ataagtcgca
     1801 aaacgctcgt gaacgcgcgc tcgaagagtt tcggagcggc gaggccaacg tgctcgtcgc
     1861 gacggacatc gcggcgcgtg gcattcacgt ctcgaacgtg tcccacgtcg tgaacttcga
     1921 tctgccgaac gtggccgaga actacgtgca tcgcatcggc cgcaccggcc gcgccggcgc
     1981 atctgggcgc gccatttctt tctgcgatca ggaggagcgt gcgttcttgg tcgacatcga
     2041 acgactgctg cgaaagcgga ttccggtagc gcaccccgcg gatccgtcga ctcaccacgc
     2101 ggccaccggc caagccgcta cgccgagcgc ttagcgcgga atcgcgctcg ccagcgccga
     2161 gggcgtggcc aacgcaacgg cgctgcgcga gccgggcgat tgacgcccca atcggagagg
     2221 gtttatcgat aacgacgtat gcccgtcgag atccgtaagc acccgattgg ggtaggcctg
     2281 tccgatttcc tgggcgtggc agactacctg ttcagagggg atccgcatta tgcgcgaccg
     2341 ctcgatctgg acaccaaaga tcggctgagc acccggaacc ccttcttcga tcatgccgag
     2401 tgcgcgctct tcacggcgca tcgcaacggc tggtgtgtgg ggcgctgctc ggcgcagctg
     2461 gatcgactgc atctcgagcg ccacggagac tcgactggct tctttggttt tttcgacacc
     2521 gtcgatgatc ccgaagtggc gagcgagctg ctgaccgcag ccgggaattg gctgcgcgag
     2581 cgcggcatga agcggattcg aggcccgctc tcgttgaact acaacgaaga ggtcggttgc
     2641 ctcgtgcagg gcttcgatgc gcccgccatg tggcttacgc cccatcaccg cgactaccaa
     2701 gcgggcttga tcgaacgcgc cggcttcacc ccgttccaag aactccacgc ctggcgcgta
     2761 gccacggacg acgtgcccga ggcggcccga agtctccacg atgggctcgc ccggctcccc
     2821 ggagtcagcg tgcggcactg caacatgaag gagttcgagg tcgatttacg cacgctgctg
     2881 gagctgcgtg acgatgcacg ccgggatagc tgggcgtttg cgccgtacac cgatcgcgaa
     2941 ctgaagcgcc tgagcgagac gctcaagtgg atcctcatcc ccgagctcac gtacctggtg
     3001 catgtcgagg ggagaccggc ggcggcagcc ttggcgcttc ccaatctgaa cgagctcacg
     3061 cgcgatctcg cgggccagct cctgccactc gggctaccaa agttgctctg gcgcctcaaa
     3121 gtctctggcc ccaaaacttt ccggatcgcg ctgctcgcgg tgcgcaaaga gtttagggcg
     3181 ctgccgcagt tcgagccgct cggcgcgatg ctcatgatca agctcaaaga agccggccga
     3241 cgttcgggct tccaactcgg cgagtggtcc tggacgagcg ctgacgatcc cgcccccgag
     3301 gccaccctgc gcgcggccgg ggccgcgacc ataaccaaga agtatcggct ttacgagcgg
     3361 gccctgacgt cttagagcct accgagagat gcccctcagc gaaggggcgt tcgaggcccc
     3421 acctcgaaca agccgtcatc aggaatccac gcgcatgaac gttttcgtca ccggagcatc
     3481 gggtttcttg ggctcacacg tggccgagca actgtccacc gccggccacc atgttcgcgc
     3541 cctcgtgcgg cgcaccagcg acactcgctt tttgagcggg ttgaacaacg tggagctggt
     3601 cgaagcccgg ctcgacgaca ccgctagcct agagcgcgcg ctgagcggat gcgagtacgt
     3661 gatccacgct gcaggcttgg tgaaggcccg ctccgagcaa gaatttttcg aggtcaacgc
     3721 gcacgcgacc gaacggctct tcgaagccgc gacacgcgtg cccggcatgc gccgcttcgt
     3781 gctcgtctcg agcctggccg tcgcgggtcc gagcgaacaa ggcgcccccg tgccgctcga
     3841 gcgaaccccc gcgccggtca cggcttacgg gcgaagcaag ctcgccggcg agcgcgccgt
     3901 gctggggaaa aagagctcgt taccgctgac gatcgtgcga ccacctgcca tctacggccc
     3961 gcgtgatcgt gaggtcttgg cgttcttccg cgcgatcaag ctcggtgtgc tgcccatcct
     4021 cggtgccccc accaatcgga tgagcatgat ttacggtccg gactgcgcgg ccgcctgcgt
     4081 ggcggcgctg agtcgagatc tgccgagtgg cacgacgttg ttcgtcgacg acggcgacgt
     4141 gcacaccttc gaagagctcg tcaccacgtc cgaggctgcg ctgggtcgcc gcgcattctt
     4201 gcgcctcccc ttaccccggc gtgtgattcg cgccgcggct ttgggcagcg agttctacgg
     4261 ccgcgtgcgc aaccaagccg tgatgttcaa ccgcgacaaa tgcaacgagc tgttcgcgga
     4321 gtgggtctgc gatggcagcg ctacccggca actgctggat tggaagcccg agttcacctt
     4381 ccgcgagggc gtgcagcgca ccgtggattg gtacaaggcc gcgggctggc tctgagagct
     4441 aacgctagta acctgcgccg ggcagcccgg caatcacgag ctgcccgcgc gttggctacc
     4501 gccgcgcctc aaatcgattt ctcgaacacg cggtaacgct tgtagatctt ggcgccgagc
     4561 gcgcggatcg cgacgttgat ggcggcgttg tcctcgagcg tgaacgagag ctctccccac
     4621 ttgtagttgc gtaacacgcc cgcatagtgc agctccgcgt acaggaaggc cgagagcccg
     4681 gcgtacttct tcactccgcg caaccgcttc gcaatcccga ggccaaccag ccgcgccgtg
     4741 tccaagccgc gccccttcag gcgccacaag aacttcgccg ccccgaacgg cagcagctgc
     4801 ccattcaggc ccgccgccgc ttcgttcagg ttggggagcg cgtaagcaaa cgcgctcggg
     4861 acaccgtcga tatcgacgat ccgagttagc tccagatcgg cgaagaactt gaattcagag
     4921 ccgatacgct ccgcttcggc gggcgtcagc ggcacgtaac cccagctatc ttgccaggcg
     4981 tcgttgtaga gctcgagcat ctgcgcgatg tctcggtcca tcgtcttctt ctcgacgcga
     5041 cggaagcgca cctcgggcat gttcttgatt tcttcgtgcg ccttctccac gcgcttggtc
     5101 aggccgtcgg ccaccacgta gcgccaggca tacgtgtctt tggccttttc gtagcccgcc
     5161 ccttccagaa gccccgcctg atagggacgg tgatgcccca tcaacaacat cggaggatag
     5221 tcgaagccct ccactaagca gccgacctct tcggccatat tcatcgtgat tggcccgcgc
     5281 agccgcttga tccccgatgc ccgatgccag ctctccaccg cgttcatcaa ggcctgcatc
     5341 acctcggggt cctcgatcgt atcgagaaag ccgaagaagc cgaggtcgtc cttgtagcgg
     5401 gccaggtgca actcgttgcg gtgagcaacg acgcgaccca cgcacttgcc atccttgtac
     5461 gccacgaaga tgcgccggcg tgcatgctgg aagtacggat gccgcaggct caagcgctgc
     5521 cagacatcca gatccagcgg ccgtatgtag ttcggatcgt cggcgtagat cgtctgaacc
     5581 acgtccagaa aatcacgaag gttttgggtc ttctcgacgg tacggatctc gatacgtgcg
     5641 ggcacgttcg gcggatacca ctgagccggg accgcgagca agcactccca aagcttggcc
     5701 atccggccag gtgcgcatag tctttgcggc cggctcgtgg atcgcctacg cctcgccaac
     5761 accatgctac cccgagttgc tgccgactat cgtaccgtcc tttgggcgtt cgtcctgttt
     5821 cccggcgtag ccctggcgca ttacgtaaac cccggactca tcggctggct gctgccgatc
     5881 agcatttacc tgggttactg cgcgggcatc ttttcgcaca atcacaacca ctgcccgacc
     5941 ttccgtagcc gggccatgaa cgacttcttc tcgatctggc tctcgatctt ctacggctat
     6001 ccgctgttcg gttggattcc gacccacaac ttgaaccacc acaagttcgt gaacaaggcg
     6061 ggcgacgcga ccatcacgtg gcgctacacg aagaagcaca ccttcctggt cgcgatcacc
     6121 tatttcttcg tctccgccta ttggcagtcg gagccaatcc gcgagtacat ccagaaggct
     6181 cgccgctcga atggtcgcat gtatcgccaa gtcgtggcgc aagcggtggc cgtgatcggc
     6241 gcgcacgtgg gcctgttggg gctcgggatt tatctgtggg gccccgctcg aggcgcgctc
     6301 gtttacgcct tcggtttcgc gatcccggcc gccttcgcgc tctggtcgat gatgttcacg
     6361 aactacatcc agcacgtgca ctgcgatccg tggtccaagg acaaccactc acgcaacttc
     6421 gtgtccccct gggtgaattg gttcgtgttc gatgccggtt accacagcgt gcatcacgag
     6481 cacgcgggca cgcactggag caagtaccgc gcgctacacg aggcccgcgc ccacc
//
LOCUS       DASTKU010001106         6472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1113731, whole genome shotgun sequence.
ACCESSION   DASTKU010001106 DASTKU010000000
VERSION     DASTKU010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113731"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFQ61_39555"
     CDS             complement(<1..282)
                     /locus_tag="VFQ61_39555"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET9960654.1"
                     /translation="MHPRIPPGLRLALNEYSERLRGKFGERLVELKLFGSYARGEAHE
                     DSDVDVLVVIDGLTDLEIGAVAGEAAPVIQKTGFLLAPLPMSILRLANLR"
     gene            complement(441..1001)
                     /locus_tag="VFQ61_39560"
     CDS             complement(441..1001)
                     /locus_tag="VFQ61_39560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET9960655.1"
                     /translation="MADPARRRASYSDLQGVPDHLVAEIVDGELVTSPRPAPRHARAA
                     SSLGGELYGPFDRGRGGPGGWVILDEPELHLAGDVLVPDLGGWRRERLPVLPEGSAFE
                     LMPDWVCEVLSPSTAPLDRTQKLPIYARERVQHVWLVDPALQTLEVLRLDGGGYRIVG
                     AWHGEATVCAEPFDVYALHLADLWSA"
     gene            complement(1615..4050)
                     /locus_tag="VFQ61_39565"
     CDS             complement(1615..4050)
                     /locus_tag="VFQ61_39565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960656.1"
                     /translation="MKQSLFVVVAAGLLLACQGPSPSSVHRNWSGVTTVELLAQVPPC
                     TLLRRGAVYYVSAEDKLLYCDGTRYQELDLRQADGAAGESWVVQILPANTVDCPNGGA
                     QMSVGPDADGDGVPDVVRETAVTCNGASAVGGPPVTRSVEEPPGTNCADGGQKLELGG
                     DVNGNGALEESEVSSVQYVCSFLRIWYSDFRAQNNADAEALRGYSTVLGDLEIGPNIT
                     DLTPLQDLTGIAGTLRLEATSLTSLEALHHLSNIGGDFVLWNTAALTSLNGLGSLTRL
                     GGSLSIRYNSGLARLTGLGPLMEIRGRLEVQDNPLLASLEGLDRVTRVGVDLSLRAGP
                     AMQNLRGLGALASVEGSVSVYGSGLIDLDGLQSLTRIGGSFSANGDRLESLFGTEHLT
                     EIGGAFHVPSSPIRRLARWDSLEHIGGDLVVEGRTGTTDLDGFNHLKTIGGSLHVYFS
                     PLTGLNGIQSLESIEGALEVRSTPIADLGALGSLRTVKGTLSLVDTRLVSLTGLALTS
                     IGGIYVASNPELADISALDSGGPLDLGEDLVLVLNDKLQSLHGLEAIRSVARDVYLSG
                     SFANIDALEGLTRVGNDFVFSNVNIDDLEGLRNLASVGGDIKTLPPVTTLEGLRELRE
                     VGGEFLVYGTRATTLNGLQNLRSAGSLRIGRNPELTQIDALSQLTEVSGTLEVFSNPA
                     LIQVDGLRNVSTVGSVDISGNASLANLLGLGGIQRIPFNFALTYNAALQTTAGLENLT
                     EVGRQLRITGNSLLLRLRGFSALERVGELLYIADNPKLASCEAEWLRDTVAARRGLGG
                     SFLSGNTGTLTCP"
     gene            complement(4047..>6472)
                     /locus_tag="VFQ61_39570"
     CDS             complement(4047..>6472)
                     /locus_tag="VFQ61_39570"
                     /inference="COORDINATES: protein motif:HMM:NF013217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960657.1"
                     /translation="ALSTLLLAHAACTERLEVKPSLELLGVTELEAIGELPACSPLWK
                     AAVFYVRSQRKLYFCDGSNYQSLALDLHPGEAGANWFVRTSDAPEAVCPAGGVMLAAG
                     PDLNGDGVFDRVASSAVVCDGTPGTNRATLTRIVEQPPGALCESGGNKIEIGFDLNGN
                     AALDEDEVTDFDYICSTSQVWMGDFEATTDAQLETLRGVSVFLGNLRISGSVTRLDAL
                     SNLREVSGDLSVSGTHLPNLEGLDNLQTLGGSLVIGGLLGNDALTDLEVLRQLTVVGG
                     DLIVENNPALTSLVGLEHFSRVGRDVSIARNHLVDLQGLHHLTAVGRHLSVFDESLLA
                     TVEALNELTEVGGNLSVFGPILQSLDGLGSLSRLGGSFWVGGPQLTRLTDFRNLTEIG
                     GNLIAPEPPLSNFRGLEHVTRIGGNLRVCQTELTDFSGLEGLETVTGNVELCTPLTTL
                     APLGSLHTVGGTLQVRSTTLTSLSGLNLRNVGGIELLYNSALVDIRAIASGSASLSLT
                     KNLWIMDNPMLRDLAGLEVISRVDGSVALGGVPNVLALRGLTRIGQNLTITSPEMRDL
                     EGLQNLTFVGGSIERLPEYLNSLKGLRGLVSVGGDLRLEHVPIRSVSALGQLTRIDGT
                     FTVYSTPLRDLQGLASLFHVGGLRISGNPQLASLDGLNAVTELGFATVAENPMLTQVD
                     ALGQVTATGGAHIASNSALRHLHGFANVETVVQSIEIVDNAVLELDSGFDAVKEIGST
                     LRIQGNGSLTSLRAFAGLERVGVSFQVLNNPNLATCEVEWLVDMLGDRRGIGTAPIYY
                     GNAGTGVCP"
BASE COUNT         1304 a         2085 c         1893 g         1190 t
ORIGIN      
        1 ccgcaggttt gcgagccgca ggatcgacat gggcagaggc gcaagtaaaa acccggtctt
       61 ttgaattacc ggtgccgcct cacccgccac cgcgccgatc tccaggtcgg tcagaccatc
      121 gatgactacc agcacgtcga catcggagtc ctcgtgcgcc tcgccccggg cataggaacc
      181 aaatagcttt agctctacga ggcgttcccc gaacttgccc cgcaaccgct cggaatactc
      241 gttcagcgcg agccggaggc caggggggat tcgcggatgc atcgccatca gattagctca
      301 ggtgtctgcg gccttctacg ataacagacc atcgaccaat agacatagac cgagtcgacg
      361 tagatcggct ctcgtgagaa ctcgcgattg gaggtgcccg gggcggtggt gtggtcggcg
      421 cgaccgcagc gcgcgttgct ctaggctgac cacaagtccg caagatgcag cgcgtacaca
      481 tcgaacggct cggcacagac ggtagcctcg ccgtgccacg cgcccacgat tcgatatcct
      541 ccgccatcta gccgcagcac ctccagcgtc tgcagtgccg gatctacgag ccacacgtgc
      601 tggacgcgct cgcgagcgta gatgggaagc ttttgcgtgc gatcgagcgg tgccgtcgag
      661 ggggagagaa cttcgcaaac ccaatccggc atgagctcga aagccgatcc ctccggaagc
      721 acgggcaggc gttcacgacg ccagccacca agatcaggga cgagaacgtc gcccgccagg
      781 tgcagctccg gctcatcaag gatgacccaa ccccctggcc ctccccgccc cctgtcgaac
      841 ggcccataga gctcgccacc caagctcgac gccgctcgcg cgtgtcgcgg cgccggccga
      901 gggctggtaa ccaactcacc gtccacgatt tcggccacta aatggtcggg aacgccttgg
      961 agatcgctgt aagaggctcg ccttcgcgcg ggatcggcca tgactggact actctagcag
     1021 cccagacggc ccgctgaatc tgccccgctt cgaaggccgg gctgaccgct ggccactgca
     1081 taaacctcaa cgatatcgcg aacacgaccc actttttcac tcggcggatg accgtggccc
     1141 ccgttttgag cagcatgatc cggactttgg agctccaatc accactctgc tcgttctccg
     1201 acgaagctcc cttctgctcc tagcgctctc gatgctcagc gcatggaacg tcagctgccg
     1261 gcacaacgcg aagcgcggga gagccaacga gggcccacac accgcgcgca cggagccggg
     1321 ccgcacttcc gtcccgcggt tgaagcgatt ctggctcgcg cccggcgagc aggttcccga
     1381 caagtccggc ggacagccgg tttcccaggt ccactcacgg ccggtgttgg tggacgtccg
     1441 aggcgttgat ttcgtgtcgt ccgtaggtga cgccccaagc gtgcgtggat tcctgcctac
     1501 tccgtcacgc actcgcgagg ccgatcgcct ggggccagtt cgcgaatcga tcacagcgac
     1561 tcgacgaatt ggccatgggt cggcgtcagc gaacgccccc ctcgattccc cagctcacgg
     1621 gcacgtcagc gtccccgtgt ttcccgaaag gaatgaacct ccgaggccgc gacgcgcagc
     1681 gacggtgtcg cgcagccatt cggcttcgca tgaggcaagt ttggggttgt cggcgatgta
     1741 cagaagttcg cctacgcgct cgagggcgga gaaaccccga agacgaagca gtaggctgtt
     1801 gcctgtgatg cggagttgac gaccgacctc ggtgaggttt tcgagcccag ccgtggtttg
     1861 tagggcggcg ttgtaggtta gggcgaagtt gaaggggatg cgctgaatgc cgcccaggcc
     1921 gagcaggttg gccagggatg cgttcccgct gatgtcgacg ctccccaccg tgctgacgtt
     1981 gcgcaagccg tcgacttgga tgagcgcggg attcgagaag acctctaggg ttcccgagac
     2041 ttcggtgagt tggctcaggg cgtcaatctg ggtgagctct gggttgcggc cgatgcgtaa
     2101 tgaccccgcg ctcctgaggt tctggaggcc gttcagggtg gtggctcgcg tgccgtaaac
     2161 caggaactct ccaccgactt cgcgcagctc gcggagtcct tcgagcgtgg tcaccggcgg
     2221 caacgttttt atgtctccgc ctacagacgc caagtttcgc agaccttcca gatcgtcaat
     2281 gttcacgttg ctgaagacga agtcattgcc gactcgagtc agcccttcga gagcgtcgat
     2341 attggcaaac gatccgctca agtacacatc ccgcgccaca cttcgaatcg cttctaaccc
     2401 gtgtaaactc tggagtttgt cgttcaggac cagcacgaga tcttccccga gatccagagg
     2461 gccacccgaa tccaatgcac tgatgtcggc aagctcggga ttagaggcga cataaatgcc
     2521 tccaatcgac gtcagcgcca ggccggtcaa gctgaccaag cgagtatcga cgagcgacaa
     2581 agttcccttt acggtgcgga ggctgcccag ggctcccaga tcggcaatgg gagtgctccg
     2641 cacctcgagc gccccctcga tactttccaa ggattgaata ccattgagcc cagtcagcgg
     2701 cgagaaatag acgtggaggc ttccgccgat ggtcttcagg tggttgaagc catccaaatc
     2761 cgtagtacca gtccgacctt cgacgaccag gtcgccgccg atgtgttcca ggctatccca
     2821 acgcgctagc ctgcgaatcg gggaactcgg gacatggaat gccccgccga tctcggtgag
     2881 gtgctctgtc ccgaacaggc tctccaggcg atcgccgttt gcgctgaagc tgcccccgat
     2941 tcgagtgagc gattgtaacc cgtcgagatc gatgagcccc gacccataga ctgagaccga
     3001 accctccaca ctcgccagcg cgccgagccc cctcagattt tgcatggccg gtccggcacg
     3061 caacgacagg tcgacgccca cgcgagtgac acggtcgagg ccctcgagcg aggcgagcag
     3121 agggttgtct tgcacctcca atctccctcg gatctccatc agtgggccga ggcccgttag
     3181 gcgcgccaag ccggagttgt accggatcga tagggaacca ccgagacggg tgaggcttcc
     3241 taacccgttt aatgacgtca acgcggccgt gttccacaaa acgaagtcac ctccaatgtt
     3301 agagagatgg tgcagcgcct cgaggctcgt cagtgacgtg gcctcgaggc gtagcgtgcc
     3361 ggctattccc gtcaagtctt ggaggggggt cagatccgtt atattggggc caatctcgag
     3421 gtccccgagc accgtagagt agccgcgcag agcttccgcg tcggcgttgt tctgcgctcg
     3481 aaaatccgag taccagatcc tgaggaagga gcacacgtat tggacgctgc tcacttcgga
     3541 ctcttccagc gccccgttcc cattcacgtc gcccccgagc tccaactttt ggccgccatc
     3601 ggcgcaattc gtaccgggag gttcctcaac gctgcgcgtg accggcggcc cgcctacggc
     3661 tgaggctccg ttgcaagtca cggccgtctc ccgcacgaca tcgggtaccc cgtcgccgtc
     3721 ggcgtcaggg ccaacggaca tctgagcccc gccgttcggg caatccaccg tatttgcagg
     3781 gaggatttga acgacccagc tctctccggc agctccatcg gcctggcgca ggtcgagctc
     3841 ctggtaccgc gtcccgtcgc agtataggag cttgtcttcc gcgctcacgt aatagaccgc
     3901 gcccctgcgg agcagcgtgc acggaggcac ctgggcgagc agctcgactg tggtcacgcc
     3961 agaccagttg cggtgcacgg aggaggggct cggcccttgg caagcgagta gtaaaccggc
     4021 cgcaacgacg acgaatagac tttgtttcat gggcaaaccc cggtcccggc gttcccgtaa
     4081 tagatcggcg cggtaccaat cccgcggcgg tcgcccagca tatcgacgag ccactcgacc
     4141 tcgcaggtcg ccaagttcgg attgttcagc acttgaaagg acacgcctac gcgctccaaa
     4201 cccgcgaagg ccctcaagct cgtgagggag ccattccctt gtattctgag cgtgctcccg
     4261 atctccttga ctgcatcgaa gcctgaatcg agctccaata cggcgttgtc tacaatctcg
     4321 atgctttgga ccacggtctc gacgttcgcg aagccatgca agtggcgcaa agcagagttg
     4381 gaagcgatat gcgcgccccc cgttgccgtc acttggccca acgcgtcgac ttgagtcagc
     4441 atggggttct cggccaccgt cgcaaaaccg agttccgtga ctgcattcaa cccatcgaga
     4501 ctcgccagct gcggatttcc cgagatccgt aggccgccga cgtggaagag gctcgccagg
     4561 ccctgcaggt cgcgcaacgg cgtggaataa accgtgaagg tcccatcgat tcgggttagt
     4621 tggccgagcg ctgagacact cctgatcggg acatgctcga gccgcaaatc gccacccaca
     4681 ctgactagcc ctcgcaatcc ctttagacta ttcaagtact ccgggagccg ttcgatactt
     4741 ccacccacga acgtgagatt ctggagccct tccaggtccc tcatttccgg gctcgtgatt
     4801 gtgagatttt gaccaatacg agtcagaccg cgcaacgcga gcacgttggg aacaccgcct
     4861 agtgcgacgg acccatccac acgggagatg acctcgagcc cagccaaatc tcggagcatc
     4921 gggttatcca tgatccacaa gttcttcgtc aaactcagac tggcagaacc cgaggcaatg
     4981 gcccgaatgt cgaccaaagc cgagttatac aatagttcga tgccacccac gttcctcaga
     5041 ttcaatccgg atagcgacgt caacgtcgtc gagcgcacct gcagtgtgcc cccgaccgtg
     5101 tgcaagcttc cgagtggtgc gagcgtggtc aggggagtgc agagttcgac gttccccgtc
     5161 acggtctcga gcccttccag tccggaaaaa tcggtcaatt cagtttggca caccctcaga
     5221 ttgccgccga tgcgagtaac atgttctaag ccgcgaaagt tgctcaatgg tggctctgga
     5281 gcgatcagat ttccgccaat ctcagtcaag tttcggaaat ctgtcagacg ggtgagctgc
     5341 gggccgccca cccagaagga cccgccgagc cgactgagcg accctagccc gtccagactc
     5401 tgcaaaatag gaccaaacac agacaagttg ccgcccactt cggtgagctc gttcagcgcc
     5461 tccacggtgg cgagcaaaga ttcgtcgaag acagataagt ggcgacccac ggccgtgaga
     5521 tgatggaggc cttgcaagtc cacgaggtga ttgcgagcga tggatacatc gcgacctacc
     5581 cgcgaaaagt gctccaagcc aacgagagac gtcaacgctg ggttgttctc gacgatcaga
     5641 tccccgccca ccacggtcaa ttgacgcagc acctcgagat ccgtcaaagc gtcgttccca
     5701 agcagacctc cgatcacgag actgccgccc agcgtctgca gattatcgag cccttccagg
     5761 tttggcaggt gggtcccact cacgctcaag tccccgctca cctccctcaa gttggaaagg
     5821 gcgtccaggc gcgtcactga tccggagatc cgaagattgc ccaagaatac cgagaccccg
     5881 cgcagagtct cgagctgggc atccgttgtg gcctcgaaat cgcccatcca aacttgcgag
     5941 gtcgaacaga tgtagtcgaa gtcggtgact tcatcctcgt cgagcgcggc gttcccgttc
     6001 aggtcgaacc caatttcgat cttgttaccg ccggactcac agagagctcc gggtggctgc
     6061 tccacgatgc gagtcagcgt ggctcgattc gtgcctggag tgccatcgca caccacagcg
     6121 ctgctcgcga cgcggtcgaa cacgccgtcc ccattgaggt cggggcccgc agccagcatc
     6181 acgcctcccg ctgggcacac cgcttcaggg gcgtcactcg tccgcacgaa ccagttcgca
     6241 cccgcttccc cggggtgaag gtcgagagcc aacgattggt agttcgaacc atcgcaaaag
     6301 tagagctttc tttggctccg cacgtagaag accgctgctt tccagagcgg cgaacacgcc
     6361 gggagctccc caatcgcctc tagctcggtg acacccagga gctcgagaga gggtttgact
     6421 tccagtcgct ccgtgcaggc cgcatgagcc agcaggagtg ttgagagagc ca
//
LOCUS       DASTKU010001107        10713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1783020, whole genome shotgun sequence.
ACCESSION   DASTKU010001107 DASTKU010000000
VERSION     DASTKU010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783020"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFQ61_39575"
     CDS             <1..244
                     /locus_tag="VFQ61_39575"
                     /inference="COORDINATES: protein motif:HMM:NF017426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HET9960658.1"
                     /translation="IEGCPFYMSVAQFDYWRHTQLIIDLVPGRGAAFSLEAPRGVRFL
                     TRSRAFSDTELAELARQGEPPRGDSEAPVETWAPFT"
     gene            473..1276
                     /locus_tag="VFQ61_39580"
     CDS             473..1276
                     /locus_tag="VFQ61_39580"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HET9960659.1"
                     /translation="MHELQPQLQLIHYDNLWQIAGSAFRYFDWGATFTWASSGAVLAA
                     RRGYDLTGIFAIGLVSSCGGGLLRDAMFLQAGPPALVRTPSYVLIALTASLLTWLAGR
                     RSSGRSGAAAGLPPWVRNAVRVADALGLGAFAVVGMRLALAASIHVAGAMLVGVVNAV
                     GGGIARSVLLREVPEVFQPGELTALAAFAGTLTYAALALGLGLDERSAGLVAIAQVSA
                     LRWISVRYHVHTRAAWSRSMREPLAEQPIQTPAPPRSSSAQSSSARPEP"
     gene            1309..2232
                     /locus_tag="VFQ61_39585"
     CDS             1309..2232
                     /locus_tag="VFQ61_39585"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET9960660.1"
                     /translation="MPPVSLSLTLRKLEYWVAIAEHQSFRKAAQACHVSQPALSAQIA
                     ELEEQLGVRLFERGARSVQLTREGAQLLEHARAVLAEAQELLEAAHQVNDPWVGRLRL
                     GILPTIGPYLLPELAPILRQRFPKLSFVWVEDKTPMLVNELARGELDGGVLALEAELG
                     SLETREFGRDPFFFAASREHPLSGTKKPIGAEEISGECVLLLEDGHCLRSQALSFCAQ
                     TGVEETSYRATSLSTLVQMVAGGPCVTLLPSLCIPVENRNQTLSLRPLREAPFRTLGL
                     VFRRGAARERTLEGVARVLGEAYAALKTHSR"
     gene            2321..3247
                     /locus_tag="VFQ61_39590"
     CDS             2321..3247
                     /locus_tag="VFQ61_39590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET9960661.1"
                     /translation="MASRDLTALNYRRIVILLVALVVVPTFLLLSLGVILIFVGEAQL
                     NVLMGILVMALSGAAVTGVILVWVFVRREANLSRLQSDFVSKVSHELRTPLTSIRLFT
                     ETLALRRGNRESEDKCIAGLERESTRLSELIDRLLDWGRMESGRREFILKDTDLRSIV
                     EAAVGAFEPVRERRSVQLDVALPAESVVVHADRGAVSDALFNLLTNAYKYGGDPAQIN
                     VAAEQRGREVRVTVRDNGGGIPKVEHSRIFQKFYRVDDRLSREREGSGLGLAIVKHVM
                     RAHKGRVELESTPGKGSAFTLVLPAVRQRGRS"
     gene            3244..3939
                     /locus_tag="VFQ61_39595"
     CDS             3244..3939
                     /locus_tag="VFQ61_39595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET9960662.1"
                     /translation="MNDTRKRILIVEDDESITLGLEMNLRAEGYDVDVAYDGETGLAR
                     AREGGLDLVILDVMLPKMNGFELLRSLRERDHTVPIVMLSARGAELDKVMGLELGAED
                     YITKPFGLAELLARVKAVLRRDGLRKNAATRVLRAGDLEIDGQSREVKRDGAPVNLTA
                     TEFDVLWCLVEASNRPLSREEILSRVWGPGHHGTLRTIDNFVLQLRNKLEKDPAEPEH
                     VLTVRGVGYRFRS"
     gene            4071..4538
                     /locus_tag="VFQ61_39600"
     CDS             4071..4538
                     /locus_tag="VFQ61_39600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HET9960663.1"
                     /translation="MDPNLLRSYLATVYELPSESGVLRVSMDGDVVRDIGTLPELLRR
                     DFAVLTAYNPRSMLLPRRVNDARHQVMRDLLIYGCYRVEHCVGYDEEPDGVFREPAWL
                     VHGIGRDEAIHFGRTFRQNTIVFCQLARPELIVTDPTCDDIGKTFQGSWRLRQ"
     gene            complement(4550..5206)
                     /locus_tag="VFQ61_39605"
     CDS             complement(4550..5206)
                     /locus_tag="VFQ61_39605"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HET9960664.1"
                     /translation="MSITVRETWIAAGAAAVLLAGAVWIGFELGRPRTPLLPAFSSST
                     AAVRESPNVVAAVQDLARLESVSFRMERVIDLADKQSHLFGLVQAEDAILLVAAADVT
                     AGVDLRRVSERDLLVDAARSRVHLKLPAPEVLSTKLDAEHTYVHTRRTGLLAQRRENL
                     ESRARQEAEQRLREAALDAGILERAGDNARRVLTALIQSLGYREVEIEIERTVREPRE
                     "
     gene            complement(5203..5943)
                     /locus_tag="VFQ61_39610"
     CDS             complement(5203..5943)
                     /locus_tag="VFQ61_39610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET9960665.1"
                     /translation="MTSSAPILSARHVHRSYGDLEVLRDLSFEVGGPQLVGILGPAGS
                     GKTSLLDLLEGLAEPSRGEIALFGKPLLPGAYPRQRVGVVLQREFLPEQICVAEYADL
                     FSAMCGVPGGASRILREAELEASAKVRVDALSTLQAQNLFIASALVHDPELLFLDEPS
                     FGVGSEAKVALGEKLRSLSRQRTVVLSTDDLSEAEKICDFCLFLSQGRLCVAAPKAEI
                     LSSMPGRSLADSFLHLCSPRSARQGQCA"
     gene            complement(6021..6557)
                     /locus_tag="VFQ61_39615"
     CDS             complement(6021..6557)
                     /locus_tag="VFQ61_39615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960666.1"
                     /translation="MMLVGRRWFVAALAFGAFAAAAFSSRAALLSQGIAGSAFGQPTH
                     SEPHASAVDTKAFSGSLVERAWSMLNGQRPTPRAQADTVDTERESDAPASMRVKEIVE
                     PWAARAATPAPPAPTDFSRAEALDPWKDGSTLHAPLPVPAKVRVSVGADPWASSCPGR
                     AACAAASAGSIVDPWATH"
     gene            6889..7647
                     /locus_tag="VFQ61_39620"
     CDS             6889..7647
                     /locus_tag="VFQ61_39620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960667.1"
                     /translation="MTTTHALAKRVCIGLLSSALLASLLERGSAADVPSVRDNLQRFK
                     DRRDAAARRAAQGGAAATGGSTQGGSTQGGSGHGGSAHGGSSEKPPLPSPSTPKPAPT
                     PTTSPKPPKPLPTPSPPPPAPPAPPLPTRPPPVPTGPPEPAPPPKPSPTGSARPINET
                     SPAENGLDELRRSRSDRRRSEVDRLRSRWGSLLMDERARSELKLHAERMARLRRMRSI
                     AEQERNLDLVRRIDELMTREERRSQSSLEALRQP"
     gene            complement(7703..8218)
                     /locus_tag="VFQ61_39625"
     CDS             complement(7703..8218)
                     /locus_tag="VFQ61_39625"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HET9960668.1"
                     /translation="MWSWCSFSRFESVARLGARAGVGARRGMGATGAAALAAALLALS
                     SAGVAAEEVSSVNVIEFRTTVNNDRGVVRCGLFRQQGWLKEPIRGAIAKIQGRSALCV
                     FNRVPNGVYGISGFHDENSNGKLDTNAIGMPTEDYCASRNARGTFGPPSFEDAKFAYS
                     GGTKRLEARMK"
     gene            complement(8643..9737)
                     /locus_tag="VFQ61_39630"
     CDS             complement(8643..9737)
                     /locus_tag="VFQ61_39630"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HET9960669.1"
                     /translation="MTRHFVSTPSTRPGTDSVLPARQTGADSIVPGRKSEVPVTLAPL
                     ASGIVRPRMPAEPTIREAPLSFLPAPSIPPGAAQDAPRVEPVSRVETVPPGPETDLER
                     ELVRQLRTGRAALPVLPQVAESALRLANDPDARIDELARLVDTDPPIAARFMSVANSV
                     AYWRGWLTASTQSAIVRLGLANTRDLLFQVVYAASTNGLKKFQAAVQRSFKRSVVAAV
                     AARTAAKELSMSGEYDYMSGLLHDIGEARVYRILDGISQAGNDRNLLKTLVQKYHCVA
                     GAEVAMAWKLPSEIVDACAAHHDESSAATPHVRLVMIADCVVQMIEARAASQPDNYAG
                     FERLGVSPELARTLVEKTRKGVGSQNTPGR"
     gene            complement(9734..>10713)
                     /locus_tag="VFQ61_39635"
     CDS             complement(9734..>10713)
                     /locus_tag="VFQ61_39635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET9960670.1"
                     /translation="DRRAKLREMLHADGYECLLLEGGDELLRVCAEFEPDLLLVAVRQ
                     PDGSGFELCGEVRASDPSRHIPVILIADDSDELAVAAGLFAGADDVILDVERTHELRA
                     RIHVQLRNKRLFDALRRVRSERDLLRRDAQTDPLTSLLNRRSLETAVSERCAARERFG
                     VLFMDIDHFKLINDRFGHDMGDRVLVAVAHILKAGLRPGDIVARYGGEEFVALIAGAG
                     PESARLVAERLRRHVEEMEPLPRGPEKVTISIGTTVFDPQKQDEAHVDLLRRADVALY
                     AAKRAGRNRVVLAPTGKSADLEDEVAPNTTVSRTPAEGTRMAPNTARSG"
BASE COUNT         1901 a         3598 c         3385 g         1829 t
ORIGIN      
        1 tatcgagggc tgcccattct acatgagcgt ggcgcagttc gattactggc ggcacacgca
       61 gctgattatc gatctagtcc caggccgcgg agcggccttt tccctcgagg caccgcgcgg
      121 ggtccgcttt ctcacccgtt cacgtgcttt cagtgacaca gagctcgcag agctcgcacg
      181 acaaggcgaa ccaccgcgcg gggactcgga ggcgccggtc gaaacttggg cgccgttcac
      241 gtgagaactc cggcccaaga gaaacacggg cgctacgcgc ccgaaagcac gcggtaacgc
      301 ggcgccgcgc ctgctctata ggcgtcggcg ctgtgttgcc aggagagcct gcctgccggg
      361 ttgccaggag gctgcctgcg gggttgccag gaggctgcct gcgggctgcg tggaaaggcc
      421 actcggaatg ccgcgcaaga tggcgcatca atgagggcgc gagaggcagc agttgcacga
      481 attacagccc caacttcagc tcatccacta cgacaatctc tggcaaattg ccggctcggc
      541 atttcggtac ttcgattggg gagccacgtt tacctgggct tcgagcggcg ccgtgctggc
      601 ggcgcgacgc ggctacgact tgacgggcat ttttgcgatc ggcttggtct cgtcgtgcgg
      661 cggtggcctg ctccgagacg cgatgttctt gcaagccggg ccccccgcgc tcgtgcgcac
      721 gcccagctac gtgctgatcg cgctcacggc gtcactcctg acttggctcg cggggcgacg
      781 ttcgtcgggt cggtccggcg cggcagctgg cttgccgccg tgggtccgca acgcggtgcg
      841 agtggcggac gcgctggggc tcggggcgtt cgcggtcgtc ggcatgcgac tcgcgctcgc
      901 ggcttcgatc cacgtcgcgg gcgccatgct cgttggggtc gtcaacgcgg tcgggggtgg
      961 gatcgcgaga agcgtactgc tacgcgaagt tcccgaggtc tttcaacctg gtgagctcac
     1021 ggcgctagcc gcgttcgcgg gcacgttgac ctatgcggcg ctagcgctcg gcctggggct
     1081 cgacgagcgt tctgccgggt tggtcgccat cgcgcaagtg tccgcgctgc gctggatctc
     1141 ggtgcggtat cacgtgcata cccgagccgc ctggagccgt tccatgcgcg agccgcttgc
     1201 tgagcagccg attcagacgc ccgcgccgcc gcgctcttcg tccgcccaat cctcttccgc
     1261 ccgtcccgaa ccctgaggcc ggattcaaat ggtaaatggt cgggctgcat gccgccggtc
     1321 tcgctgtcac tcacgctgcg caagctcgag tactgggttg cgatcgcgga gcaccagagc
     1381 ttcaggaagg cggcccaagc ctgccatgtg tcgcagccgg cgctctcggc ccagattgcc
     1441 gagctcgagg agcagctcgg agtgcgcttg ttcgaacggg gggcgcgttc cgtccaactc
     1501 acgcgcgaag gggcgcaact gctcgagcac gcgcgggccg tgctcgccga agcgcaggag
     1561 ctgttggagg ccgcccatca ggtcaatgat ccgtgggtag gtcgcctgcg gctcggcatc
     1621 ctgcccacga tcggaccgta tctgctgccg gagctcgcgc cgatcctccg ccagcgcttc
     1681 cccaagctct cgttcgtgtg ggtcgaagac aagaccccaa tgctggtcaa tgagctcgcg
     1741 cgcggggagc tcgatggcgg cgtgctcgcg ctcgaagccg agctcggcag cttggagacc
     1801 cgtgagttcg ggcgcgatcc gttcttcttt gccgcttccc gggaacaccc actgtccggc
     1861 actaagaagc caattggcgc cgaagaaatc agcggcgaat gcgtgctgct gctcgaggac
     1921 gggcattgct tgcgctcgca agcgctgtcc ttttgcgctc aaacgggggt cgaagagacg
     1981 agctatcgcg ccaccagcct ttccaccttg gtccaaatgg tggccggtgg accgtgcgtc
     2041 acgctgctcc ccagcctgtg tatcccagta gagaatcgca accagacctt gagtttgcgg
     2101 cctctgcgcg aggccccgtt tcgaacactg gggctggttt tccggcgggg agcggcgcgc
     2161 gagcgtacgc tagaaggcgt ggcgcgagtc ttgggcgaag cttatgcggc gctgaagacg
     2221 cactcgcgct agccaccgct cgccttggtt ctccgttctc gcgcgacccg gcccggatag
     2281 cgagagctcg ccggcttggc tatactgccg gcgttctggc atggcttcgc gtgacctgac
     2341 cgccctcaac tatcggcgca tcgtcatcct gttggtggcg ttggtcgtgg tgcccacgtt
     2401 tttgctgctt tcattgggag taatcctgat cttcgtgggc gaagcgcagc tcaacgtgct
     2461 gatgggcatt ctggtcatgg ccctcagtgg cgccgcggta acgggcgtga ttttggtttg
     2521 ggtgttcgtg cggcgcgagg ccaacttgtc gcgcttgcag agcgatttcg tgtccaaggt
     2581 ttcgcacgag ctgcgtactc ccctcacctc gatccgactg ttcacggaga cgctcgcgct
     2641 gcggcgcggg aaccgcgagt cggaagacaa gtgcatcgca ggcttggagc gtgagagcac
     2701 gcgtttgtcc gagctcatcg atcgcttgct cgattggggt cgtatggaaa gcggacgacg
     2761 ggagttcatc ctgaaagaca ccgatttgcg atccatcgtc gaggccgccg tgggcgcctt
     2821 cgagccggtg cgagagcggc gcagcgtaca gctggacgtc gctttgccgg ccgaatcggt
     2881 cgtcgtgcac gcggaccgcg gagccgtctc ggacgccctc ttcaacctgc tcaccaatgc
     2941 ctataagtac ggcggcgatc ccgcgcaaat caacgtggcg gccgaacagc ggggccgaga
     3001 agtgcgagtc acggtccgtg acaacggggg cggcattccc aaggtggagc attcgcggat
     3061 ctttcagaaa ttctatcgag tcgatgatcg cttgtcgcga gaacgcgagg gctcggggct
     3121 gggcctcgcc atcgtcaaac acgtgatgcg agcgcacaaa ggtcgagtgg agctcgaaag
     3181 cacgcccggt aaaggcagcg cgttcacgct ggttttgccg gcggtgcggc aacgggggcg
     3241 gtcatgaacg acacgcgcaa acgaatcctg atcgtagagg acgacgagag catcaccctc
     3301 gggctcgaga tgaacttacg ggccgaaggt tacgacgtgg acgtcgccta cgatggtgag
     3361 acgggcctgg cgcgcgcccg tgagggtggc ctcgacctcg tgatcttgga cgtcatgctc
     3421 cccaaaatga atggcttcga actcttgagg tcgctccggg aacgcgacca cacggtaccc
     3481 atcgtgatgc tcagcgcccg tggcgcggag ctcgacaagg tcatgggcct cgagctcggc
     3541 gcggaagact acatcaccaa acccttcggc ttggccgagc tcctggctcg cgtcaaagcc
     3601 gtgctgcggc gcgacggtct ccgcaagaat gcggcaaccc gcgtcctgcg cgccggagat
     3661 ctggagattg acggccagag tcgcgaggtg aaacgcgacg gcgctccggt caatctcacc
     3721 gccaccgagt tcgatgtgct gtggtgtttg gtagaagcgt ccaatcgccc cctctcgcgt
     3781 gaggagatcc tgtcccgagt ttggggcccc ggtcatcacg gtacgctgcg caccatcgac
     3841 aacttcgtgc tgcagctgcg aaataagctg gagaaggacc ccgccgagcc cgaacacgtg
     3901 ctgaccgtcc gaggcgtggg ttaccgtttc cggagctaga cccggaagac ggttcttgtg
     3961 ccgcgagcgc gagccgctca cgatgctgcg gtggacggcc ggcctagagg gacccaagcc
     4021 gcgtcgagca gcagtgcatc cgcgcctggc gtgctataag cgcgcccgca gtggatccga
     4081 acttactccg ctcctacctc gccacggtct atgagctgcc ctcggaaagc ggagtgctgc
     4141 gcgtgtccat ggatggcgac gtcgtacggg acattggcac gctgcccgag ctgttgcggc
     4201 gcgacttcgc ggtgctgacc gcctacaatc cgcgcagtat gctgctgcca cgtcgggtca
     4261 acgacgcgcg gcaccaagtc atgcgggacc tgctgattta cggctgttac cgagtcgaac
     4321 actgcgtcgg ttacgacgaa gagccggatg gtgttttccg cgagccagcc tggctggttc
     4381 atggcatcgg tcgtgacgaa gccatccact tcgggcgaac gttccggcag aacaccatcg
     4441 tgttctgcca gttggctcgc cccgagttaa tcgtcactga tccaacttgc gacgacatcg
     4501 gcaaaacttt ccagggcagc tggcgactac gccagtaacc cggagggctt cactcgcgcg
     4561 gctcgcggac cgtgcgctcg atctcgatct cgacctcgcg gtaacccaag gattgaatca
     4621 acgccgtcag caccctgcgc gcgttgtcac ccgctcgctc gaggatccct gcgtccagcg
     4681 ccgcctcccg caggcgctgt tctgcctcct ggcgcgccct ggactcgaga ttctcgcggc
     4741 gttgcgcgag cagccccgtg cgccgcgtgt gtacgtacgt gtgctccgca tccagcttgg
     4801 tgctcagcac ctcgggcgcg ggcagcttca aatgcactcg gctgcgcgcc gcatccacca
     4861 gcaagtcgcg ctcgctcacg cggcgcaaat ccacgcccgc ggtcacgtcg gccgcagcca
     4921 ccagcagaat cgcgtcctcc gcttggacta acccaaacag gtgagattgc ttgtccgcca
     4981 aatcgatcac ccgctccatt cggaacgaca cgctctccaa gcgcgccaag tcctgaacgg
     5041 cagcaaccac gttggggctc tcgcgcaccg ccgccgtgga cgagctgaag gccgggagca
     5101 aaggcgtacg cggccgcccc aactcgaacc caatccagac cgcaccggcc agcagcaccg
     5161 ccgccgcccc cgcggcaatc caagtctcac gaaccgtgat gctcacgcac actgcccctg
     5221 cctcgccgac cgcggcgaac acaggtgcag aaatgaatcc gcaaggctgc ggccgggcat
     5281 cgaactcaga atctctgcct tgggtgccgc gacacagagc cgcccctggg aaaggaacag
     5341 acagaagtcg cagatcttct cggcttcgct cagatcatcc gtgctcagca cgaccgtacg
     5401 ttgccggctc aacgaacgca gcttttcccc gagggccacc ttggcctcgc tacccacgcc
     5461 aaaactcggc tcgtccagga acaacaactc gggatcgtga acgagcgccg acgcgatgaa
     5521 cagattttga gcctgcaggg tcgagagcgc gtccaccctc accttggccg acgcctctag
     5581 ctctgcctcg cgcaggattc gggaagcgcc ccctggtacg ccgcacatcg ccgaaaacag
     5641 atcggcatac tccgcgacac agatctgctc gggtaagaac tcacgctgca agaccacgcc
     5701 gacccgctgc cgaggataag cgcccggcag gagcggcttg ccgaacagcg cgatctcgcc
     5761 gcgcgaaggc tccgccaagc cctccagcaa atccaatagc gaagtctttc ccgagcccgc
     5821 cggccccagg atcccgacga gctgcggtcc gccgacttcg aagctcaaat ccctgagcac
     5881 ttcgagatca ccgtagctgc ggtggacatg acgagcactg agaatcggag cgctggatgt
     5941 catttgaact cgtggtcgac ctggcgatcc cgactggtct ggcgatctgg ttgactggca
     6001 accgacgaag tcacaccaag ctaatgcgtt gcccagggat ccacgatact gcctgcgctc
     6061 gccgcggcac acgcagcgcg cccgggacaa gaggatgccc aaggatccgc accaacgctc
     6121 acgcgcactt tggcgggcac cgggagggga gcgtgcaacg tggagccatc cttccaagga
     6181 tccaaagctt cggcacgaga aaaatcagtc ggggccggcg gagctggcgt ggcggctcga
     6241 gctgcccaag gctcgacgat ctctttcacg cgcatcgacg cgggagcatc cgactcgcgc
     6301 tcggtatcga ccgtatcggc ctgagctcga ggcgtggggc gttgcccgtt cagcatgctc
     6361 caggcgcgtt cgacgaggct gcccgaaaac gcctttgtat ccaccgccga ggcgtgcggc
     6421 tccgaatgtg taggttgccc gaatgcagac cccgcgatgc cttgcgaaag cagggcggct
     6481 cgagagctga atgccgccgc agcgaaggcg ccaaacgcca acgcagccac gaaccatctc
     6541 cgcccgacca gcatcatcga gatcttaagc caagggattg ttcggtcaaa acaaaacttc
     6601 ccgagttccg ggcgcttcca agctcccact tgccaccacc tgccgtccca acgagtgaat
     6661 cgcgaccgcg agcagccggg tgtgaccggc cgcaatgcgc tcggccatcg cgctctcgag
     6721 ttctcgcgcg cccggcgctg cgcaataaca tcggcaccga atggctaccg tcgccgacaa
     6781 acctgccccg cgagccggtg ttacatcacc cttgggtgca aacggcgagg cgaacgcgcc
     6841 cacgtatgca gcccgtttta aggctacgtt tgcaacctga ggagcgccat gaccaccacg
     6901 catgctctcg ccaaacgggt ctgcattggg ctgctgagca gcgctttgct cgcgagcctg
     6961 ctcgaaagag gttccgccgc ggatgtcccg agtgttcggg acaacctgca gcgcttcaaa
     7021 gatcgacgtg acgcggccgc gcgacgtgcc gcccaaggag gcgctgccgc gaccggtggt
     7081 tcaactcaag gtggttcaac tcaaggcggc tctggccacg gtggctctgc ccacggtggc
     7141 tcatccgaaa agcctccgct gccctcgccg tccaccccga agcccgcacc caccccaacc
     7201 acctctccca agcctcccaa accgctgccc acgccctcac ctccgccacc cgcgccccct
     7261 gcgccgccgc tccccacccg acctccgccc gtaccgacgg ggccccccga acctgcgccg
     7321 ccccccaaac catcccctac cggctcagcg cgacccatca acgagaccag cccggccgag
     7381 aacgggctcg atgaactgcg tcggtcgcgt tctgatcgtc gacgtagcga agtcgatcgt
     7441 ctgcgctccc gctggggctc actgctcatg gacgagcgcg ctcgctccga actcaagctc
     7501 cacgccgaac gcatggctcg actgcgccgc atgcgcagca tcgccgagca agagcgaaac
     7561 ctcgacctcg tgcgccgcat cgacgaactc atgacgcgcg aagaacgccg cagtcagagc
     7621 tcgctcgaag ccctacgcca gccgtgaccg agcccttcga aatggtccga ggactcgcgc
     7681 catccgggga gggacggaga gctcacttca tccgcgcttc gagccgcttc gtaccgcccg
     7741 agtaagcaaa cttcgcgtcc tcgaagctcg gtggcccaaa cgtgccgcgc gcgttgcgcg
     7801 aagcacagta atcctcggtt ggcatcccaa tcgcgttcgt atcgagcttg ccgttcgagt
     7861 tctcgtcgtg aaaaccggag atcccgtaaa ccccgttcgg cacgcgattg aagacgcaca
     7921 gcgcgctgcg cccttgaatc ttggcgatcg ccccgcgaat cggctccttc aaccagcctt
     7981 gttgccggaa caacccgcaa cgcaccacgc cccgatcgtt attcacggtc gtccgaaact
     8041 cgatcacatt gacgctcgaa acctcttccg ccgccacccc tgcgctcgaa agcgcgagca
     8101 gcgccgcggc caacgcggcc gcaccggtgg cgcccatacc ccgccgcgcg cctacacctg
     8161 ctcgggcgcc caagcgggcc accgactcaa accgagaaaa cgagcaccaa ctccacatga
     8221 ctgcgaccta gatctggagg cgggccggtc aggtgtcaac tgtcgcccgc acgaactcac
     8281 aacgtgggca attcacggcg cggccggttc ggagcgcccc acgagtttcg ttcgagttcc
     8341 gctcgaggtt tgctcggagc tctggcgcgg agcacgtgtt tagtctggcc gcccgcggta
     8401 acgggcgcga acttgcgccg aggtcacacc gccgctcagt ccattcgacg cgtccgtgag
     8461 tcgattcacc gcgctggcgg cgttcaacaa cgcacgcggg tgatcaacga cgttacccga
     8521 caggttacga ggtgcggcct gcgcggcatg gggggacccc gagtgagcct cgtcggcaac
     8581 ggcgccggac ccgccgagta gtcgcgctcg taacagtggg gccgcccaat tcaggaggcg
     8641 aatcaacggc caggggtgtt ttgggagccc acacccttgc gcgtcttctc gaccaaggtg
     8701 cgcgccagct ccggggagac ccccaggcgc tcgaagcccg cgtaattgtc gggctgggac
     8761 gcggcgcgcg cctcgatcat ctgcacgacg caatcggcga tcatgaccag acgaacgtgg
     8821 ggcgtcgcgg ccgaggattc atcgtggtgc gcggcgcagg cgtccacgat ttccgacggg
     8881 agcttccaag ccatcgcgac ttcggcgccc gcgacgcagt ggtacttctg aaccaaagtc
     8941 ttgagcaggt tacggtcgtt tccggcctgg ctgatgccgt ccaggatcct gtacacgcgg
     9001 gcttcgccga tgtcgtgcag caagccgctc atgtagtcgt actcgccgct catcgacagt
     9061 tctttggctg cggtgcgggc cgccaccgcc gccacgacgc tgcgcttgaa cgagcgctgg
     9121 acagcggctt ggaacttctt gagaccattg gtggaggcgg cgtagaccac ctgaaacagc
     9181 aggtctcggg tgttggccaa gccaaggcgc acgatcgcgg attgggtgga cgcggtgagc
     9241 cagccccgcc agtaagccac gctgttcgcg acggacatga agcgcgcggc gatgggcgga
     9301 tcggtgtcca cgagtcgcgc gagctcgtcg atgcgcgcgt ctggatcgtt ggcgaggcgc
     9361 agcgcggact cggcaacttg gggaagcacg ggcaacgccg cgcgcccggt gcgcagttgg
     9421 cgcacgagtt cgcgctccaa gtccgtctcc ggcccgggcg ggacggtctc gacacgcgat
     9481 accggttcta cgcgcggagc atcttgcgcc gcaccggggg gaatcgaggg agccggcaaa
     9541 aaggagagcg gcgcttcgcg aatggtcggc tccgcgggca tacgcggacg cacgatgcca
     9601 gaagccagcg gcgccaaggt taccggcact tcactcttgc gcccgggcac gatggagtcg
     9661 gcgccggttt ggcgcgccgg aagcaccgag tccgtgccag gccgcgtcga aggtgtcgaa
     9721 acgaaatgtc gtgtcatccc gacctcgctg tattgggcgc cattcgagtg ccctcggcag
     9781 gggtgcgtga aacggtggtg ttcggcgcga cttcgtcctc gaggtccgcg ctcttgcccg
     9841 taggagcgag cacgacgcgg tttcgacccg cgcgcttggc ggcgtaaaga gccacgtcgg
     9901 cgcggcgcaa taagtccacg tgcgcctcgt cttgtttttg cggatcgaag acggtggtcc
     9961 cgatgctgat cgtgaccttc tcggggccgc gcggcagagg ctccatttcc tcgacgtgac
    10021 ggcgcaaacg ctccgcgacg aggcgggccg actccgggcc cgcaccggca atcaaggcca
    10081 cgaactcttc tccgccatag cgagccacga tgtcccccgg gcgtaagccc gccttgagga
    10141 tatgcgcgac ggccacgagc acgcggtcac ccatgtcgtg cccgaagcga tcgttgatca
    10201 gcttgaagtg gtcgatgtcc atgaagagca cgccgaagcg ttcacgagca gcacaacgct
    10261 cgctcacggc cgtctccaaa gatctccgat tgagcaaact cgtcaagggg tccgtctggg
    10321 catctcgtcg caatagatcg cgctcactcc gcacgcgacg gagggcgtcg aacaggcgct
    10381 tgttacgcag ttgtacgtga atgcgcgcgc gcagctcgtg ggtgcgctcg acatccaaga
    10441 tcacatcgtc ggcgcccgcg aacagccccg cggcgaccgc gagctcgtcc gagtcgtcgg
    10501 cgatcagaat gactgggata tgacggctcg ggtcgctcgc tcgtacttcg ccacacaact
    10561 cgaagccact cccatctggc tgccgaacgg cgactagcag gagatcgggc tcgaactcgg
    10621 cacacacgcg cagcagctcg tcgccaccct ccagcaacaa gcattcgtaa ccgtcggcgt
    10681 gcagcatctc gcgcagcttg gctcgtcgat cgt
//
LOCUS       DASTKU010001108         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_1783458, whole genome shotgun sequence.
ACCESSION   DASTKU010001108 DASTKU010000000
VERSION     DASTKU010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783458"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1014)
                     /locus_tag="VFQ61_39640"
     CDS             complement(205..1014)
                     /locus_tag="VFQ61_39640"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET9960671.1"
                     /translation="MLPVPGGTFQMGADDEGEQDERPRHAVTVKSFLLDVTEVTHGAY
                     SECVKASACSAPDSLAGSALTSGHPEEFRKPDHPVSGVAWADAVKYCEFRGKRLPTEA
                     EWERAARGDDGRRYVWGNDAPDPKRHGVFGGRPTTDGVGKFTDGKGPYGHLDLAGNVW
                     EWVADLYDPYAYRRETASQGIPGSCEQILETLSYLRRHHLQGFTGKNPIPSECERVLR
                     GGAYNYGTAMMRASNRVHHPASFRIAVAGFRCARDLPSPPSGCTASTGASP"
     gene            complement(1208..1897)
                     /locus_tag="VFQ61_39645"
     CDS             complement(1208..1897)
                     /locus_tag="VFQ61_39645"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HET9960672.1"
                     /translation="MQRPYSQTPMPRAQRGSPRMFESDFFEYFSRVHPLVPAGIYGPI
                     ALLSLGVAVGVHHVSVVRVLLEFVGGYLFWTLFEYWLHRLFFHLPVVGPKTQRIYFYV
                     HGVHHDYPWDEGRLVIPPGASLFLCGVIYLTFRVLFGAHDMYGPFAGFVFGYVLYDTT
                     HWYVHAHAPKNRFGRWLRREHMLHHFKEPTSRFGVSCPWLDYVFGTRGRPHSRAAEDA
                     TSGSTSVGAAG"
     gene            2167..>2719
                     /locus_tag="VFQ61_39650"
     CDS             2167..>2719
                     /locus_tag="VFQ61_39650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960673.1"
                     /translation="MSRAKLALERFGNHVRTRFKHDRKHLLLEVVAAIVLAISLFAFL
                     RSWLTSYSPALFDPNLQTDDARTALFPFHRYAKGHPLLHDPIANEMIQYQPHAFRLIY
                     FIAVPLVGLLWASKVAQFVCLGIMLAGAVVLARSRRAGLGTAALFIFLFLRDGFVMDR
                     VGGGLPRSFGFPAMALWLAGALAH"
BASE COUNT          509 a          895 c          826 g          489 t
ORIGIN      
        1 tgcaaggcgc gcgatagaga cactctcagg ggagcgtcgt catctactac cagaactcgt
       61 ggagcagcct ctgacaacgg atcctccggg tctcgttccc aacggtcaaa caggaaaaac
      121 ctttagtcga ccaacggcag actcttggag tcgaaacacg ccagttccct gccaagcgtc
      181 aggctcgcaa ccgtaagggt ggttttacgg actagccccg gtggacgccg tgcatccgct
      241 gggagggctc ggtaggtcgc gcgcgcagcg aaaacccgcg acggcgatcc taaagcttgc
      301 agggtgatgc acgcggttcg aggcgcgcat catcgctgtg ccataattgt acgcgccgcc
      361 ccggagcacc cgctcgcact cggacgggat cgggtttttc ccggtaaacc cctgaaggtg
      421 atgccgccgt aagtacgaga gcgtctccag gatctgctca cagctaccgg gaatcccctg
      481 actcgctgtc tcgcgtcgat aggcatacgg gtcgtagaga tccgctaccc actcccagac
      541 gttgccagcc aaatccaggt gcccatacgg gcccttcccg tcagtgaatt tacctacacc
      601 gtcggtcgta ggtcgcccgc caaatactcc gtgccgcttg gggtctggag catcgttgcc
      661 ccacacgtag cggcgtccat cgtccccgcg cgcggctctc tcccactccg cctcggtagg
      721 caggcgcttg ccgcggaact cgcagtactt cactgcgtcc gcccatgcca ctcccgaaac
      781 cggatggtcg ggcttgcgaa actcctccgg atggcccgac gtgagtgcgc tccccgcgag
      841 cgaatccggc gcggaacaag cggacgcctt cacgcactcg ctgtacgcac catgggtcac
      901 ttcggtgacg tcgagcaaaa agctcttcac cgtcaccgcg tggcgtgggc gttcgtcctg
      961 ctcgccttcg tcgtccgcgc ccatttgaaa agtgccgccg ggcactggca gcatgtcact
     1021 cggaatctct ggcagcgggc acggctgcgt ttgcgatggc gaggcaaccg ggctcgcttc
     1081 agggcgaggt ggagtcgcgg ccggcgtgct cgcggtcgcc gatggagcag cagtgcgctc
     1141 cggagtgaga acgatcgcgc gcggcgcttc gttgcaagcg aagaacgccg cggtcgctaa
     1201 gagcgcttca gcccgctgcg cccacgctgg tcgagccact cgtcgcgtct tccgctgcgc
     1261 gcgagtgcgg acgccctcgc gtaccgaaca cgtagtcgag ccatgggcag ctcacgccga
     1321 aacgactcgt cggctccttg aagtgatgca gcatgtgctc gcgccgcaac cagcgaccga
     1381 accgattctt cggagcgtgt gcgtgcacgt accagtgcgt ggtgtcgtag agcacgtatc
     1441 cgaacacgaa gcccgcaaac ggaccgtaca tgtcgtgcgc gccgaacagc acccggaacg
     1501 tcagatagat aaccccgcaa aggaacagcg acgcgcccgg ggggatcacc aaacgcccct
     1561 cgtcccacgg atagtcgtgg tgcacgccgt ggacgtagaa gtagatgcgc tgagtcttcg
     1621 gcccgaccac cggcaagtgg aagaagaggc gatgtagcca gtattcgaag agcgtccaga
     1681 acagatagcc gcccacgaac tccagcaaga cgcgcaccac cgaaacgtgg tggacaccaa
     1741 ccgctacgcc caagctgagg agcgcgatcg gcccgtaaat cccggcaggg accagcggat
     1801 gaacgcgcga gaaatattcg aaaaaatcgg actcgaacat acgcggcgac ccgcgctgag
     1861 cgcggggcat cggagtttga gaataaggcc gctgcatgct ctccctacca acgaatacgc
     1921 gcgctaacgt gctcccagag gcttcggact gcaaggggtg tgtgagttct ggccccgcgg
     1981 ccgcacgcaa aagtcaactc gtacgaacgg ctggcccgtg gttcagcgca aagtgcgtcg
     2041 agtcgtacgc gcttcaaccg gaagggtcag agcgcttcgc ggttcgcagc taggcgcgca
     2101 cgctattgcc gagacaactt tgctcgggta gcaacccgcc ctgtgccaag ccgaatccga
     2161 gctttcgtga gccgagccaa gctggctctc gagcgattcg gcaaccacgt ccgtactcgc
     2221 ttcaagcacg atcgcaaaca cctcctgctc gaggtggtcg ccgcgatcgt gctcgcgatt
     2281 tcgctcttcg cgtttctacg gtcctggctc acgagctaca gccccgctct cttcgatccc
     2341 aatctgcaaa cggacgacgc gcggactgcg ctgtttccgt tccatcgcta cgcaaaggga
     2401 cacccgctcc ttcatgatcc catcgcgaac gagatgattc agtatcagcc gcacgctttt
     2461 cggctgattt acttcatcgc ggtgccgctc gtcgggctgc tctgggcctc caaagtcgct
     2521 cagttcgtgt gcttggggat catgctcgcg ggtgccgtcg tgctcgcgcg gagccggcgc
     2581 gccggactcg gaacggcggc gctcttcatt ttcttgttct tgcgtgacgg cttcgtcatg
     2641 gaccgcgtgg gcggtggtct gccgcggagc ttcggttttc cggccatggc gctctggctc
     2701 gccggcgcgc tcgcccaca
//
LOCUS       DASTKU010001109         6587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_221778, whole genome shotgun sequence.
ACCESSION   DASTKU010001109 DASTKU010000000
VERSION     DASTKU010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221778"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFQ61_39655"
     CDS             <1..889
                     /locus_tag="VFQ61_39655"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HET9960674.1"
                     /translation="EVAELWRARELHRLAVRREAGARDWLTRWVSPRYELGHLGNHAV
                     AQAEADLTSAAVDLERARARLLAAEAALTTPELKFDMRLLDQYEQAFSTESTSSSDWR
                     QLESTAPNRSPSLLAARERASAQRTQASATGLRVLSPITGLVDVRPSRTSTDTETSEA
                     RRTTSSTERLLEFSVTVPFKPRELGSLIHAGARARLGSAQAERIASELVLTLKQLRSE
                     ADSRQSQLRAARERKRAAELAYAEVERRYRAAEELTALDDVRRMQLELFAADRELIQA
                     RAATLEIEAQFRSLTRGNQ"
     gene            886..2187
                     /locus_tag="VFQ61_39660"
     CDS             886..2187
                     /locus_tag="VFQ61_39660"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HET9960675.1"
                     /translation="MTVQPFLSASKFPSARIGLSLAVYLGSGCLAEKEYIENECTPHK
                     STLALIENAARKAQSPYEAPVVPECAPLDRPPDGNEFGPGIVFEEYAAMISGTSGNFL
                     DNGTWWGKALPRGELHIQPTKLYDENCSVLDYDHFQYDYAALTPAVSSVPECGRALVG
                     LSIGHRFFQSNPQVLELDATSYARLVPLKLNTIFGTSFRLATENAAHAGETFPALEEI
                     HLLRHDADSLSVAATVSGRSVEGAFEAELVPGASSQLRVNLTLYRRTGADPKIRIGVF
                     GMSSMFWKRDDETPDQPQDEAHDCDEFVVHFADGTGRTVPLENPPRTEIPDSWTRHTF
                     SGPALQGSEPDGAALASVTLRQSERDFKRYLAYESARYAERPTMHVEILESNAPLVAE
                     LSVSHADSEYIDNVVASVFVDPSFSFEEPVHVRYVLSATPD"
     gene            complement(2216..3505)
                     /locus_tag="VFQ61_39665"
     CDS             complement(2216..3505)
                     /locus_tag="VFQ61_39665"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HET9960676.1"
                     /translation="MNSLGVMHGTLSIGVGILAAGAVVSCGNSERSGRTGAQLCDDRA
                     RLADRRAEAEANARLEFVSQNAESCLELDRPEFDSSESSGEVAGLDAGEYAALVRDAN
                     GNFYDDGTWWLKGAPRGEVHVDPVRLSSERCESVTFERLGFNYSAARPPIWDVPECGK
                     YLTGLYAGSRTGSSWPQLLHIDGSGWTRLAALEKEPAFGSYVRLLGSGPANGDGSARV
                     IGIDVVTVDSEALRLRLSLDGPDFSGLMDALLAPGNPSRLFVEVSLFPRAGRPLSRLP
                     LATLSGGFLRGRSDTPENDTDEAHDADRIEVQWDDESRSGWDWAELVGGARGSGWETL
                     AAGEPGDRVKSMALIQSERAPERYAAEPEARYARRPDLGIREIRSDLPLFVELAFSAR
                     PMLEGNAAVTLMLDATRHDSGPIRVSYIVEAAVPTLL"
     gene            4172..>6587
                     /locus_tag="VFQ61_39670"
     CDS             4172..>6587
                     /locus_tag="VFQ61_39670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET9960677.1"
                     /translation="MSLVIAIEAQDPIAAELRALLGEHVQCVSLDAPKLDCRLLVHAP
                     PAHDSLKILAASCATRGIPEQPVVVVTRDEGRHFRLAALAAGAMECVAFANLSCQCLL
                     GAIEDAIERFALFRRSAAFRATFENAAIGLAHVALDGRFLAANGALCKLTGYTLEELR
                     SKTFSDITHPEDLKSDWDQAHRLLSGDISSYSMEKRYLRKGGSEVWIRLNGALARDEH
                     GLPFFIAVIEDIDARKATEREHRKLLELAEHSCDFISTADLDGHLTYLNPGGREMIGL
                     PAETDIRRVHLTDYVAAPWRAFCENTLLPTVRKQGMWSGEMQFVNLRGEQVDVSRLSF
                     LLRDPDTGKPWCIATVARDIGDRKRAERALAEADERLRAQLSASDVGLWNWDARQNRV
                     NVDPNLIRLFGLSADDDPADLNVYLARIHPEDRERVGTNILNSVQSGASYDQVYRVLR
                     PEGGVRWIHARGLIERDPSGRPLSFPGIALDVSERYETEARLRESESILRSLYESSVF
                     MMGVVEIPDDDSDILHIYDSPATYRFFGVAPGSMDRKWSSEIGVPSAVSARWIATYRE
                     SEREKRAVRFEYEHPGPSGPSSLVSVVTCIGRAPHGGTRFSYVTEDVTERKRSEQRLR
                     DRDIHLGLAMAASLTIAFDWDIRSNRVWRPRSVEQALPETGEDWDTFEGVASVVHPDD
                     RELFHANIREALRSSSGKYRSEHRIVRQDGEIRWLFETGTVEFDADRNPVRLLGVSQD
                     VSDRRQEQETLQRQAQLIDISYEPIMLWTRDRGILEWNWGAERLYGFTKAEALGESVH
                     ELLQTRS"
BASE COUNT         1292 a         2081 c         1986 g         1228 t
ORIGIN      
        1 cgaggtcgcg gagctctggc gagcccgcga gctccatcgc ctggccgttc gtcgagaagc
       61 gggcgccagg gattggctca ctcgctgggt aagcccccgc tacgagctcg ggcacctcgg
      121 aaatcacgcc gtcgctcaag ccgaggcgga cctgacgagc gccgccgtgg atctcgaacg
      181 ggcccgcgcg cggttactcg cggccgaagc tgccctcacc acacccgagc tcaagttcga
      241 catgaggcta ctcgatcaat acgagcaagc attctcgacg gagtcgactt ccagcagcga
      301 ctggcggcaa ctcgaaagta cggcaccgaa ccgtagcccg tcgctgctcg ccgctcgcga
      361 acgcgcgagc gctcaacgca ctcaggcctc ggctacgggc ctgcgcgtgc tgtccccgat
      421 tactggtctc gtggacgtca ggccatcacg aacctccacg gataccgaga ccagcgaagc
      481 gcgtcgcacc acctcatcta ccgagcggct gctggagttc tcggtgaccg tacccttcaa
      541 gccccgcgag ctcggcagtc tcatccacgc gggggcgcgg gcgcgtctgg gatcagcgca
      601 ggccgagcgc atcgcgagtg agctcgtatt gaccctgaag cagctacgca gtgaggccga
      661 ttcacgccag agccagctgc gcgccgcccg ggagcggaag cgggctgcgg aactcgccta
      721 tgcggaggtc gagcgtcgct accgagcagc cgaggagctc accgccctgg atgatgttcg
      781 acgtatgcaa ctggagctgt tcgccgcgga ccgtgagcta atccaagcac gcgcagctac
      841 cctcgaaata gaagcgcagt tccgcagcct gacccgaggc aaccaatgac cgtgcagccc
      901 tttctatcgg cctctaagtt tccttcggct cgcatcgggc tcagcttggc cgtgtacctg
      961 ggctcgggtt gcctcgccga gaaggagtac atcgagaacg agtgtacccc gcacaaatcg
     1021 acgcttgccc taattgagaa cgccgcgcga aaagcgcagt caccgtacga agcccccgtg
     1081 gtgccagaat gtgctcccct cgaccgcccc ccggatggta acgagttcgg accaggtatc
     1141 gtgttcgagg aatatgcagc catgatctct ggcacctcgg gcaatttctt ggataacggc
     1201 acgtggtggg gcaaggcgtt accgcgtggc gagttgcata tccaaccgac caaactgtac
     1261 gacgagaatt gcagcgtcct cgattacgat cacttccaat acgactatgc cgccctcacg
     1321 ccggcggttt cttccgttcc agaatgtggt cgtgcgttgg tgggtttgtc gattggccat
     1381 cgcttctttc aatccaatcc tcaagttttg gagttggatg ctacttccta tgcacggctg
     1441 gtcccgctca agctcaacac gatcttcgga acttcttttc ggcttgcgac cgagaacgcc
     1501 gcccacgccg gcgagacatt cccagcgctg gaggagatcc acttgcttcg ccacgatgcg
     1561 gattccttga gcgttgcggc cacagtttcc gggcgctccg tagaaggtgc gttcgaagcc
     1621 gagctcgtgc ccggagctag ctcgcagctg cgcgtgaatc tgaccctcta ccggcgaacg
     1681 ggtgcagatc cgaagattcg catcggagtt ttcggcatga gctcgatgtt ttggaaacga
     1741 gacgatgaga cccccgatca gccgcaagac gaagcgcacg actgcgacga gttcgtcgta
     1801 cacttcgccg atggcacagg acgaaccgtc ccgctcgaga accctccgag gacggagatt
     1861 ccagatagtt ggacacggca caccttctcg ggtccagcgc tccagggctc cgaacccgat
     1921 ggcgctgcgc tagccagtgt taccctgcgc caatccgaac gggacttcaa acgctacctc
     1981 gcgtatgagt ctgcgcggta cgcagagcgg cccacgatgc acgtagagat cttggagtcc
     2041 aacgctcccc tcgtcgccga gctctcggtc tctcacgcgg actccgagta catcgacaac
     2101 gtggtcgcaa gcgtgttcgt agacccgagc ttttcattcg aagagcccgt tcacgtgcgg
     2161 tacgtattgt cggccacgcc ggactgattc tcgagcgcgc cggtgggggg tacgctcaga
     2221 gcagagtcgg gacggccgct tcgacgatgt aagagacgcg gattggcccc gaatcgtgtc
     2281 gagttgcgtc caacattaga gtcaccgcgg cgtttccctc gagcatgggc cgtgcagaaa
     2341 aagccagctc cacgaacaag ggcagatctg aacgaatctc gcgaatcccg agatcgggac
     2401 gtctcgcgta ccttgcttcc ggctcggccg cgtagcgctc gggcgcccgt tccgactgaa
     2461 tcaaggccat cgatttcact cggtcacccg gctcgcccgc ggccaacgtt tcccaacctg
     2521 atccacgagc gcccccgacc aactcggccc agtcccagcc tgaccgtgat tcgtcgtccc
     2581 actgaacctc gattcgatcg gcatcgtgcg cctcatccgt gtcattctca ggcgtatcgc
     2641 tgcggccccg aaggaagcct ccactcaggg tggcgagcgg caaccgactc aacgggcgcc
     2701 cagccctcgg gaacagactc acctccacga agagacgaga tggattgccc ggcgcgagga
     2761 gcgcgtccat caaaccagag aagtcagggc catcgagaga gagtctgagc cgcaatgcct
     2821 cgctatcgac cgtgactacg tcaatgccga tgactctcgc tgagccgtcg ccgtttgcgg
     2881 ggccactccc gagcagccga acgtagctcc cgaacgcggg ctccttttcc aatgcggcca
     2941 agcgtgtcca gcctgagcca tctatatgga gcaactgcgg ccatgaactg cctgtccggc
     3001 tgcccgcgta tagtccagtg aggtatttgc cgcactctgg aacatcccaa atcggcgggc
     3061 gcgcagcact gtagttgaac cctagccgtt cgaaggtcac ggactcacat cgctcactcg
     3121 aaagccgcac cggatcgacg tgtacctccc cccgcggagc ccctttcaac caccacgtgc
     3181 cgtcgtcgta gaagttccca ttcgcatcgc ggacgagggc ggcatattcc cccgcgtcca
     3241 ggcccgcgac ttcaccggac gattcagagc tatcgaattc aggccggtcg agttccaggc
     3301 aggattcggc gttttggctg acgaactcga ggcgggcgtt ggcctctgcc tccgcgcgtc
     3361 tatctgccag acgcgcgcgg tcatcgcaga gctgtgcccc tgtgcgaccg gagcgttcgc
     3421 tgttgccgca gctgacgact gccccggccg cgaggatccc cactccgatc gagagggtac
     3481 cgtgcattac accaagagag ttcaagagtg aaaggctcga ccgcaagaag cgcgctccga
     3541 tacacatatc aatcttcagt atctaatgca caccgggcac gagcgccatc ccatagaatc
     3601 actcctgagc gtgagaagaa gaccgagcgg gtgattgatc gcctgttcga ccgtgggctc
     3661 cgcatccatc aacggcgaga catcgaaacg gcggcgccct tttgctcgac acattgaccc
     3721 agccctcggc ttcccttgct cactccgcgg tgggtgttct cacgcagcgg tcgagggcga
     3781 tgatgcggct gccaactaac tcagtcccta tgactcagtc cctacgaccc agccccgatg
     3841 attattttcc ccgaacgagt ctctccactt tactgagttt gcaccccgca gaggtaggtg
     3901 cgcggccagg ggagaatggt cgagcaggcg ggcgccaact cattggcaca gagcccgatc
     3961 cgggcgtccc tgtcgaagtt cacgcggagc gttggttttc gactggtaca aggaaggaac
     4021 ggcgaaccag aagagcaacc atcctctggc gctgatcggg cggctactac acgaattggc
     4081 ccgtatttcg gtaccgcgaa gagtgctata aagacgaggt cgcccaacgt atcacgtggc
     4141 gctccgccca gcgtgcgaag ccacgcgccc catgagcctc gttattgcaa tcgaagccca
     4201 agatccgatc gccgctgagc tacgagcgtt attgggcgag cacgtccaat gcgtgtcgct
     4261 agacgcaccg aagctcgatt gccggctgct cgtacatgca ccgccggcgc atgactcgct
     4321 gaagattttg gccgcctcct gcgcgacgcg agggatccca gaacaacccg tcgtcgtcgt
     4381 cacgcgagac gagggccggc atttccgact cgcagcgctc gccgcgggcg ccatggagtg
     4441 cgtcgccttc gcgaacctga gctgccagtg tctgctcggc gcgatagaag atgccatcga
     4501 gcgttttgct ctattccgca ggtcggctgc gtttcgtgcc acgttcgaga atgcagcgat
     4561 cggtcttgcg cacgtcgcac tcgacggaag gtttctggcc gccaatggtg ccctgtgtaa
     4621 gctgactggc tacaccctcg aggagctccg cagcaagacc ttcagcgaca tcactcaccc
     4681 tgaagacctc aagtcggatt gggatcaggc tcaccggttg ttgagcggtg atatctcgtc
     4741 gtattccatg gaaaagcgct acctgcgtaa gggaggtagc gaggtttgga ttcggctgaa
     4801 cggcgccctc gcgcgcgatg agcacgggct gcccttcttc atcgccgtga ttgaggacat
     4861 cgatgcgcgc aaggctaccg agcgcgaaca tcgcaagttg ttggagcttg cggagcacag
     4921 ctgtgacttc atttctactg ctgatttgga cggacatctg acgtatttga acccgggtgg
     4981 gcgagaaatg attgggctcc ctgccgagac cgacatccgt cgcgtccacc tgactgacta
     5041 tgtcgccgca ccatggcgag cattctgcga gaacaccttg ctaccaaccg tgcgcaaaca
     5101 gggcatgtgg agcggagaaa tgcagttcgt caacttgcgc ggcgagcagg tcgacgtctc
     5161 gcgactcagc ttcttgctcc gagatccgga taccgggaag ccttggtgta tcgcgacggt
     5221 ggcgcgagat atcggcgatc ggaagcgcgc cgagcgcgcg ctcgcagaag ccgacgagcg
     5281 actccgggcc cagctctcgg cgagcgatgt cggactgtgg aattgggatg cgaggcaaaa
     5341 tcgagtcaac gtcgacccca acctgattcg gctcttcgga ttgagcgccg acgatgatcc
     5401 ggccgatctc aatgtctact tggcccgcat tcaccccgag gatcgcgagc gcgtgggcac
     5461 gaacatcctc aactcggtgc agtccggcgc atcctatgac caagtctatc gagtgctgcg
     5521 cccggaaggc ggggtacgct ggatccacgc gcgaggcctg atcgagcgcg acccgagcgg
     5581 aaggcccttg agctttcccg gcatcgcgct ggacgtcagc gaacgctacg agacggaagc
     5641 gcgcctccgc gagagcgaat ccatcttgag gagtctgtac gagagctcgg tcttcatgat
     5701 gggagtagtg gagattcccg acgatgactc ggacattctg catatctacg attcgccggc
     5761 tacttaccgc ttcttcgggg tggccccggg gagcatggat cgcaaatggt cgagtgaaat
     5821 aggtgttccc agcgcggtgt cggcgcgttg gatcgctacc taccgcgaga gcgagcgtga
     5881 aaagcgggcg gtgcgcttcg aatacgagca ccccggccca agcgggccgt ctagcctggt
     5941 ttccgtcgtc acgtgcatcg gccgcgcgcc gcatggggga acccgcttct cctatgtgac
     6001 cgaagacgtc accgagcgta agcgatccga gcagcgcctg cgagaccgtg acatccatct
     6061 cggcttggca atggctgcgt cactgaccat cgcgttcgat tgggacattc gcagcaatcg
     6121 agtttggcga ccgcgcagcg tcgagcaggc gctgcccgag acgggtgaag attgggatac
     6181 attcgagggt gtcgccagtg tggtgcaccc cgatgatcga gagttgtttc acgcgaatat
     6241 tcgcgaggcg ctacgctcga gtagcgggaa gtatcgttcc gagcaccgca tcgtacgcca
     6301 ggatggtgag attcgatggt tgttcgagac cggaaccgtg gaattcgacg ccgatcgcaa
     6361 tcccgttcgc ctactcggtg tatctcagga cgtgagcgac cggcggcagg aacaggagac
     6421 actgcaacgt caggcgcagc tgatcgacat atcttacgag ccgatcatgc tgtggacgcg
     6481 agatcgcggc atcctcgagt ggaattgggg cgccgagcgc ctttacgggt tcacgaaggc
     6541 cgaggcactc ggagagtccg tccacgagct gctgcaaaca cgctctc
//
LOCUS       DASTKU010001110         8277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_667250, whole genome shotgun sequence.
ACCESSION   DASTKU010001110 DASTKU010000000
VERSION     DASTKU010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667250"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFQ61_39675"
     CDS             complement(<1..436)
                     /locus_tag="VFQ61_39675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HET9960678.1"
                     /translation="MFGSGSRGFAVLAVVASLLGLLFASYSTSDYASHLDRRLHDVHC
                     SFIPGAAPTSEAEACRAAMYSPYSALFKESLWGGVPISLFALGAFTFFAGFALYLWVA
                     GSRAPRLALIFFGAISLTPLLVSLVMLAISVTQLGHLCKTCVG"
     gene            complement(551..2404)
                     /locus_tag="VFQ61_39680"
     CDS             complement(551..2404)
                     /locus_tag="VFQ61_39680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960679.1"
                     /translation="MAASALGAASTQPSPSSVSIDDIDSDWTSDDLPPVAPSTTDQVP
                     KEMLRSPSFPADPAVPADLRPPVKSVRSLRPTLKLGDAPLQTAMTPEAPAAQVPEGVY
                     QGASALAAAAMSPAAVSPAAVSPAAMSPAASGPSVAPTSVGLTPQPSRPDGSPRDNAP
                     RDSSAREVSSSRETSARETSARDTEPGRESWSVAPPSKPRASRPSLPPIPRITSGVSS
                     MPPRVEPIIPSAPNPMPERSGSDARSRPTVPNRPSALRPPAPATEDKAPESKNAVAPS
                     ESQSPSEAQAAAAQATLDALLGPTSRSGIQTSAVSAEGVSLTAERSRESEAPQSAAQV
                     AEAGLQTSIANIPAEFSRNTPTPAPRFKPEPELLQGTPVNTARPSANPWTAGASAAPN
                     GVEKEFLAATALRSNPRHKYVFGACLAAAAVVLWIVTRPAPRPPELRSQAASPAAEMP
                     SLPIAAESKDRPTQRAATASESDVAAAENAEKKAGASETGTASEPSPPAGANAANAAR
                     DTAQPREEARADASQPSSGPAAAEGSAASASDAAKGDKVFVHITAKPADVQLFYKGKS
                     IGKPPLTIELKPGETRTFEAILAGHTTRKVRVDGTKPEMMIGLRPVSTPSP"
     gene            complement(2776..3906)
                     /locus_tag="VFQ61_39685"
     CDS             complement(2776..3906)
                     /locus_tag="VFQ61_39685"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET9960680.1"
                     /translation="MSAGSSRSWVGYASVLLCGAAVGCGGAQKSTASVAARPVNAAAV
                     SQLAQGAEAAKQPEGRRRAMGLFESAVKTDRELFEAHFNLGVLKAEAGDLEQAERSLS
                     EAERLAPNAEDVVVALAEVRRRRGEPQAALDSLRRFVKDNPNATLAPIVLMAVLRESG
                     KADQAIDLAHQVLVRRARDPYALSELALAHLERAEVDTAEILAQEAAKADPKSAVAER
                     TLGLVALKKGDDALSFRHFARATELDPTDTTARLNMGTVLLQAGVYDRAAEQFRAVLT
                     ANAEDTPATLGLAAALRGGAKKDDSRALQEAEKLLTALLVREPGNWAAQFNLALLYSG
                     PLKRPDAASALFKRVLDSAPSQHPAHAEAEKYLAGTGKNAGP"
     gene            complement(3903..6974)
                     /locus_tag="VFQ61_39690"
     CDS             complement(3903..6974)
                     /locus_tag="VFQ61_39690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960681.1"
                     /translation="MRIPERLRRVFGAALERRIDGDVRDTRRLRREAMSLLEAFLRDA
                     DPTAAEMPEALLRAAELEWEEARDRFVAAFQRWEATPTDQRGNPPVPEYARPRARVRR
                     VLQQYKSYADYDLALYVDGFLASEEGKTEESLGRFEKIIEWFPKSRFVPDAHMMRAEY
                     EFTKSAPDYETAYREYERVLEFKDSELYDLALFKSAWTLWRLGKSEEAARRFLTVFKS
                     TNERSSPARAGSAKKSQRELDELQSEALKNLVAVFVEDEQNRAEDMHRFLVQAGGDKF
                     AARIVKALAEAFYDQAHYERGVEAYRLLIKLEPVSPEAYKYALAIAEAHSTMEAWAAL
                     EQDYRAIVKEYLPPSAAGTGAGSQSKASAWVSVQSSATLARASAAIERQLREDAIGLH
                     AKAQADKTSRAEFEGAARLYGVYLSRFQAGPQAYETYFNLAEIQFYRLENASEAAAAY
                     LAAVRLDPKGPLSRNALYNALAALEVARAAEFESDRKANRKSSETPNDKKITEAMELY
                     VKNYPGDAQIPELLFRQGKLYYDYQVFDPAVRQWGLLLEKYPNHAYAGTAGELILDSF
                     NKSRDYTNIETWARRLKTAPQFQTAAQQQRLEALIVGAVFKQGEQLAESGEHAKAAAA
                     YVRAAAEFPREERAAQAAVNAEVEAKRAGDLATLQRAVELLLKHHKNRSETAQGLWIA
                     ATTHQEVGLFSEAANYHARIAEGFPKFQNHKDAAFNAVLLRTSIGEHDKAIDSGEKFK
                     KDYARDEAADDVTFLMGKAHEKAGKKSEAAALYENYAKSARSPSGQIESLVRLSMVST
                     EERTRSQALERAIHVYSTHKSQLDGRGKYHAAQARYLQGQAFLEKFEAVKIEGDVQKL
                     KERLRKKSDLLKKAADAFLATAELGVAEWTTAALYQIGNTYESFSRALLTSPPPEKLG
                     AEEKELYKQSLEEFVIPIEERSLEAYESGWQKAIELGIFNAWTAKMREALGRLNAELY
                     PPLKETGFELRSRGPMPLPALIDAPRRDRSGRSERYLIQTGESAANASEAP"
     gene            7777..8175
                     /locus_tag="VFQ61_39695"
     CDS             7777..8175
                     /locus_tag="VFQ61_39695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET9960682.1"
                     /translation="MSLARILVIDDDAVVTRMLSRVLSGHDVIVTESAEDALELLKSQ
                     QFDLILCDLAMPGMSGMAFYEAVRALSAKILEKIVFMTGGAFTTEGHEFLSQLPHSAW
                     LEKPFDIGALREMIRVRAASSDGKANGRAQ"
BASE COUNT         1403 a         2745 c         2559 g         1570 t
ORIGIN      
        1 ttcctacgca ggttttgcag aggtgcccga gctgagtgac gctaatcgca agcatcacca
       61 gcgacaccag cagcggggtc aggctaatcg ctccgaagaa gatcagcgcg aggcggggcg
      121 cccgggagcc ggcgacccag agatagagcg cgaacccggc gaagaaggtg aaggcaccca
      181 acgcgaacag agagatcgga accccgcccc acaacgactc cttgaacaac gccgaatatg
      241 ggctgtacat cgcggcccga cacgcctctg cttcggaagt gggcgccgca ccggggatga
      301 agctgcaatg cacgtcgtga agccgtcggt ccaagtgcga ggcgtaatcg gacgtggaat
      361 aggatgcgaa cagcagcccg agcagggacg cgaccactgc caagaccgca aaaccacgag
      421 agccagaccc gaacatccgg ccgagagttg caacggcgga ggctcgggtc aagccggaac
      481 tgaacggaaa gctgctcgaa atctccgctg aggctgatcc cgggagccat tggccccgca
      541 caaagtagct tcaggggctc ggggtgctaa ccggcctgag cccgatcatc atctcgggtt
      601 tggttccgtc gacgcgaact ttgcgggtgg tgtggcccgc taagatggcc tcgaacgtgc
      661 gcgtttcacc gggcttgagc tcgatggtga gcggcggctt accgatgctc ttgcccttgt
      721 aaaagagctg tacgtctgcc ggcttcgcgg tgatatgcac gaacacttta tcgcccttcg
      781 ccgcgtccga ggctgaagct gcgcttcctt ctgcggctgc gggcccagaa gagggctggc
      841 ttgcatcggc acgcgcctcc tccctgggct gcgcagtatc gcgggcggcg ttcgcggcat
      901 tggcgccggc gggaggcgaa ggttctgaag ccgttcccgt ctccgacgcg ccagccttct
      961 tctctgcgtt ttcggcggcc gcgacgtcac tttcggaggc cgtcgctgcc cgttgcgtcg
     1021 gtcggtcctt gctctccgcc gcgattggca gagaaggcat ctctgccgcg gggcttgccg
     1081 cttgcgatcg caattccggt ggcctgggtg ccggtcgcgt tacgatccag agcaccaccg
     1141 ccgcagccgc caaacacgcg ccgaaaacgt acttgtggcg cgggttggag cgcagcgccg
     1201 tcgcggccaa gaactccttt tcgaccccgt tcggcgcagc gctagcccca gccgtccacg
     1261 ggtttgccga aggccgcgcg gtgttaaccg gcgtgccttg cagcagctcc ggctccggct
     1321 tgaacctggg cgccggggta ggcgtatttc ggctgaattc cgcgggtata ttcgcgatcg
     1381 acgtttgcag ccccgcctcc gccacttggg ctgcgctctg gggtgcctcg ctctcccggc
     1441 tccgctccgc ggtgagcgaa acgccctccg ccgagaccgc cgaagtctgg attccgctcc
     1501 ggctagtggg accgagcagc gcatccaacg tcgcttgcgc ggccgcagct tgcgcctcgc
     1561 ttggcgactg gctctcggaa ggagcgactg cgtttttcga ttccggcgcc ttatcctcgg
     1621 tcgctggcgc gggcggcctc aacgcagaag gccgattggg gactgtaggg cgactacgag
     1681 cgtcggaacc cgaacgctcc ggcatcggat tcggcgccga tgggatgatg ggctcgacgc
     1741 gcggaggcat ggaagagacc ccgctcgtga tgcgcggaat cgggggcagg ctgggtcgac
     1801 tggcccgcgg cttcgagggc ggtgcaactg accaagattc gcggccaggc tctgtatctc
     1861 gcgcagaagt ctctcgcgcg gaggtctcac gcgaagaaga aacctcccgt gcggaagagt
     1921 cccgcggagc gttgtctcga ggagaaccgt cgggacgaga gggttgaggc gtcaacccaa
     1981 ctgaggttgg agccaccgaa ggacccgacg cggcgggcga cattgccgcg ggcgacactg
     2041 cggcgggcga cactgccgcg ggcgacattg ccgcagccgc cagcgccgat gctccctggt
     2101 aaacgccttc aggaacctgc gccgcaggcg cctcgggggt catcgccgtt tgcagcgggg
     2161 cgtctcccag cttcagagtc ggtcgcagcg agcgcaccga cttcacgggc ggcctcaaat
     2221 ccgccggcac cgcgggatcc gcagggaagc tcggtgagcg aagcatctcc ttcggaacct
     2281 ggtcggtcgt ggacggcgcc acgggcggca agtcgtccga tgtccaatcc gaatcgatat
     2341 catcgatcga gaccgagctg ggtgaaggtt gcgtcgaagc ggcccccagg gcactcgcag
     2401 ccatgggccc gctaatcgtc accacaggat gaggagccac cgatccggtg gccagcgtca
     2461 ccccactcgc cggaggcaag gcaccggtag gcggctgact ggtctgccgc tcgcccccat
     2521 cggaggcttc aggagaagaa ccacgagcct tcgctccagc tggctggtca cccatattgc
     2581 tccgcgcgag catagttgaa aaagcgcgcc cggacaccac gagagtaggc tattcgaata
     2641 cccctccgta aaattcgtgt ctggcttcag acggaagcgt tactggtgtt gctcgaaacg
     2701 acgtccgtca atcgcgcggt ctgcgttcgg tgaagtcgcc tgcgaggtca gcttccagtc
     2761 acccgcgacg caacgtcagg gcccggcatt ctttcccgtt ccggccagat acttctccgc
     2821 ttccgcgtgc gcggggtgct ggctaggcgc ggaatccaac acacgcttga agagcgcgct
     2881 ggcggcatcg gggcgcttga gcggtcccga atagagaagc gccagattga actgagccgc
     2941 ccaattgccg ggttcgcgca cgagcagtgc cgtcaaaagc ttctcggctt cttgcagggc
     3001 gcgggagtca tctttcttgg cgccgccgcg caaagcggcg gcgaggccca aggtcgctgg
     3061 agtatcctcg gcattggccg ttagaaccgc gcgaaattgc tccgccgcgc gatcgtatac
     3121 gccggcctgg agcagcaccg ttcccatgtt caggcgcgcc gtcgtgtccg tgggatcgag
     3181 ctcggtcgcc cgcgcgaaat gacgaaagct cagcgcgtcg tcgcccttct tgagcgccac
     3241 cagccccaag gttcgctccg cgaccgcact cttcggatcc gccttcgccg cctcctgggc
     3301 caagatctcc gccgtgtcca cttccgcgcg ctcgagatga gcgagcgcca actccgaaag
     3361 tgcgtacgga tcgcgggctc gccgcacgag cacctggtgc gccaagtcga tggcctggtc
     3421 ggccttgcca ctttcgcgca gcacggccat gagcacgatc ggggccagcg tcgcgttggg
     3481 gttgtccttc acgaagcgcc gcaacgagtc cagcgccgcc tgtggttctc cgcgtcggcg
     3541 tcgcacctcc gccaacgcca ccaccacgtc ttcggcattc ggagccaacc gctccgcttc
     3601 actcagcgat cgctccgcct gctcgagatc gcccgcctcc gctttcagga ctcccaagtt
     3661 gaagtgggct tcgaacagct ctcgatcggt cttcacggcg gactcgaaca agcccatcgc
     3721 ccgccgccga ccttcgggtt gcttggccgc ctccgcgcct tgagccaact ggctgaccgc
     3781 ggccgcattc acgggccggg ccgcaacact ggccgtgctt ttctgggccc cgccgcaacc
     3841 cacggcggcc ccgcaaagca gtacgcttgc gtaaccgacc cagcttcggc tcgacccggc
     3901 gctcatgggg cctcgctcgc gttcgccgcg ctttcgccgg tttgtatcaa gtatcgctcg
     3961 ctgcgcccgc tgcggtcgcg acgaggcgcg tcgatcagcg cgggcaacgg catggggcca
     4021 cgcgaacgca gttcgaaccc agtttctttc agcggcgggt agagctcggc gttcaagcga
     4081 ccgagagcct cacgcatctt cgccgtccaa gcgttgaaga tcccgagttc gatcgccttt
     4141 tgccaaccgc tctcgtacgc ctccaagctg cgctcctcga tcggaatcac gaactcttcg
     4201 agtgattgct tatacagctc tttttcctcc gcgccgagtt tttcgggtgg tggtgaggtc
     4261 agcagcgccc gcgagaaaga ctcgtacgta ttgccgattt ggtagagcgc agccgtcgtc
     4321 cactcggcca ccccgagctc cgccgtggcc aggaatgcgt ccgccgcctt cttcaacaga
     4381 tcgctcttct tacgcagccg ttccttgagc ttctgcacgt ctccttcgat cttgacggcc
     4441 tcgaacttct cgaggaacgc ctgcccctgc aggtagcgcg cctgggccgc atgatacttg
     4501 ccgcgcccgt cgagttgcga cttgtgagtg gagtacacgt gaatggcccg ctccaaagct
     4561 tggctgcgcg tgcgctcttc ggtactcacc atcgaaagcc gcaccagcga ctcgatctgc
     4621 ccgctcgggc tgcgcgcgct cttcgcgtaa ttctcgtata gcgccgcggc ctcgctcttc
     4681 ttgccggctt tctcgtgggc cttacccatc aggaaggtaa cgtcgtccgc ggcttcgtcg
     4741 cgcgcgtagt ccttcttgaa tttctcgccg ctgtcgatcg ccttgtcgtg ctcgccgatg
     4801 ctggtgcgta gcaggacggc gttgaaggcg gcgtccttgt gattctgaaa tttcggaaag
     4861 ccctccgcga tccgcgcgtg gtagttcgcg gcttcgctga agaggccgac ttcctggtgc
     4921 gtggtggcag cgatccaaag gccctgcgca gtctcgctgc ggttcttgtg gtgcttgagc
     4981 aacagctcga ccgcgcgctg caaggtcgcg aggtcccccg cccgcttggc ctccacttcc
     5041 gcattgacgg cggcctgcgc agcacgctct tcgcgaggaa attcagccgc agcgcgtacg
     5101 tacgcggcgg cggctttggc atgttcgccg gactcggcga gctgctctcc ttgcttgaac
     5161 acggcgccca cgatcagggc ctccagccgt tgttgctggg ccgcggtttg gaactgcggc
     5221 gcggtcttca agcgacgcgc ccacgtctcg atgttcgtgt agtcgcggct tttgttgaat
     5281 gagtccagga tcagctcgcc cgccgtgccc gcataggcgt ggttcgggta tttctcgagc
     5341 agcagtcccc attgccgcac ggccggatcg aatacctggt agtcgtagta cagcttgcct
     5401 tgtctgaaga gcagctccgg gatctgtgca tctccggggt agttcttcac gtacaactcc
     5461 atcgcctccg tgatcttctt gtcattcggc gtttcgctgg acttacggtt ggccttgcgg
     5521 tcactctcga actccgcggc gcgggcgacc tccaacgccg cgagcgcgtt gtagagcgcg
     5581 ttgcgagaca gcggcccctt tggatccaag cgcaccgcag ccaagtaagc ggcggcggcc
     5641 tcgcttgcgt tctccaagcg atagaactga atctccgcca gattgaagta cgtctcgtac
     5701 gcttggggcc ccgcctgaaa gcgagagaga tacacgccgt agagccgagc cgcgccttcg
     5761 aactcggcgc ggctcgtctt gtccgcttgg gccttggcgt gcaacccgat cgcgtcctcg
     5821 cgcagctggc gttcgatggc agccgaggct cgagcgaggg tggcggagct ctggacggag
     5881 acccacgccg aagctttgct ttggctgccc gctcccgtac ccgccgcgct gggaggcaag
     5941 tactccttca cgatcgcgcg atagtcctgt tcgagggccg cccacgcttc catcgtagag
     6001 tgggcctccg cgatcgcgag cgcgtatttg tatgcctccg gactgacggg ctcgagcttg
     6061 atcaacaacc gatacgcttc gacaccccgt tcgtagtggg cctggtcata aaatgcctcc
     6121 gccagcgcct tcacgatccg cgccgcaaac ttgtcaccgc ccgcctgcac cagaaatcgg
     6181 tgcatgtctt cggcgcgatt ctgttcgtct tcgacgaaca ccgccaccag gttcttgagc
     6241 gcctcgctct gaagctcgtc cagctcgcgc tgcgacttct tggccgagcc agcgcgtgcg
     6301 ggtgagctgc gttcgttggt cgacttgaac acggtcagaa agcggcgcgc agcttcctcc
     6361 gatttgccga gccgccacag cgtccaagcg ctcttgaaca gagcgaggtc gtacaactca
     6421 ctatctttga actccagcac tcgctcgtac tcgcgatacg ctgtctcgta gtccggtgcg
     6481 ctcttggtga actcgtactc ggcccgcatc atgtgtgcgt ccggcacgaa gcggctcttc
     6541 ggaaaccact cgatgatctt ctcgaaacgc ccgagagatt cttctgtctt gccctcctcg
     6601 ctcgccaaga agccgtccac gtacagcgct agatcgtagt ctgcgtacga cttgtactgc
     6661 tgcaaaacgc gcctcacccg cgcgcgcggg cgggcgtact ccggtaccgg cgggttaccc
     6721 cgttgatccg tcggcgtggc ttcccagcgc tgaaaagcgg caacgaatcg atcccgcgcc
     6781 tcttcccact cgagctccgc ggctcgcaac agcgcctcgg gcatctccgc cgcggtcgga
     6841 tcggcgtctc tcaggaatgc ctccaagagt gacattgctt cacgtcgcag ccgtcgtgta
     6901 tcgcgcacgt cgccgtcgat tcgccgctcg agcgcggcgc cgaacacgcg tcgcaagcgc
     6961 tcgggaatcc tcaagctcag ctggatgcct tgctgcgcct tttccgctcg ctgcgtccgc
     7021 ttcctgacag tgggacgctc acgtacacct aggctgcgtt cgcccgccgg ccgaatccca
     7081 gctcctggcg cagctccggc gggggcgctg ctggggcttg gtccgccagc gtccttgctc
     7141 gccccgtgtc caacgctggg tgtagcaaca atgccaaaca gagtggccac caccaccatc
     7201 gtgttcgtgc gccgaaaaag cttcgcagcg gaacacgcgg cagagtgctc tgtcggtttc
     7261 attttttccg tcgacaggat accggtctca acaaagttgc gcaattcgcc aggttcaccg
     7321 catgctcacg cgccgcgctc ggcgtgagta cggccgagtg aatgctcgcg tgttttccaa
     7381 tgaaaagccg cgaggaaagc cacgaaaaac accacgcgat aattgcatcg attcagccac
     7441 cgaggggtga acagcgctga gttgcgcacc gggagcaacg catgtcggcc cgagtcgcac
     7501 ccggcaaaca gccacaatgc gctagcgaaa tgctggatcg aataccggca atgcccgaca
     7561 cgcatcactc cgaaggtggc tatccgacca ccgcgctacc accttcgcga aagcgatcgc
     7621 acgaacgaaa gacgtaacgc gctagcccga atcgagacaa acgaggtgtg ggccacaatc
     7681 cacgacgatt ggcatttcga gagcgcctcg cttcttgtaa ccttggcgat acggctcttg
     7741 gtcgagacta ggagccgaac gaatcacgga ggaggaatgt ccctggcaag gatcctggtc
     7801 atcgacgatg acgcggtcgt aacccgcatg ctcagccgag tattgtcggg tcacgacgtg
     7861 attgtgacgg agagtgccga ggacgcgctc gagttgctca agtcccaaca attcgatctc
     7921 attctgtgtg atctcgcgat gcccggcatg agcggcatgg cgttctacga agcggtacgc
     7981 gctctctccg cgaagattct ggagaagatc gtgttcatga ctggaggtgc gttcacgacg
     8041 gagggacacg agtttctgtc gcagctccct cactcggcgt ggctagagaa gcctttcgat
     8101 atcggcgccc tgcgcgagat gatccgagtg cgtgcggcgt cctcggatgg caaggccaac
     8161 ggccgagctc aatagcttca cgttaccggg cgctcagctt cgccgagcgc tcagggccgc
     8221 ggcggctcag cgcaaacctt cgccccccac atactcgtcc gcccagtctt caccgtc
//
LOCUS       DASTKU010001111         3872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Polyangiaceae bacterium isolate SMAG_U2256
            k141_444642, whole genome shotgun sequence.
ACCESSION   DASTKU010001111 DASTKU010000000
VERSION     DASTKU010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970016
            Sequence Read Archive: SRR25158480
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Polyangiaceae bacterium (soil metagenome)
  ORGANISM  Polyangiaceae bacterium
            Bacteria; Pseudomonadati; Myxococcota; Polyangia; Polyangiales;
            Polyangiaceae.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 22:47:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,942
            CDSs (total)                      :: 7,861
            Genes (coding)                    :: 7,839
            CDSs (with protein)               :: 7,839
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 4 of 22
            Pseudo Genes (incomplete)         :: 20 of 22
            Pseudo Genes (internal stop)      :: 2 of 22
            Pseudo Genes (multiple problems)  :: 4 of 22
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Polyangiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444642"
                     /isolate="SMAG_U2256"
                     /isolation_source="soil"
                     /db_xref="taxon:2268199"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="27.756161 N 118.023465 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1884)
                     /locus_tag="VFQ61_39700"
     CDS             complement(<1..1884)
                     /locus_tag="VFQ61_39700"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HET9960683.1"
                     /translation="MSTDRGKNPEPTESSPCAARVEAARTQSGESESARPQSARAFAG
                     HPVDVATGEQFTAAHDVEVPGTSPLIFRRVYNTAFLKRAPTVLGPGWVHAFEASLKRD
                     LDGYVFEGHDGDRVEFDDVDGTFSSGREGASLIDRAACMELRREGEQLVIYHWHSVDE
                     PVQKYVFNTRDGDFMWLAARVLPSGQGLRIERDRLGRATAITQTQERRRLHLAYDNQG
                     RLSTLHLGLVAQTAREARLVARYEYDAQSRLIAVFDAHGAAQRYAYDNEHRLIAETGR
                     RGGVFRMRYDAQGRCIETSGEGGYRKCVFHYEPGRTTRVTDSLGFDTIYQYNAQGQVH
                     HKILPNGAQYVTELDDYGRIVGEIDPLGKATRYVYDDVGRVAEKIFPNGAKLQYEYDE
                     YHQPKRITEPDGAVWEFGYERGALVAVKDPFERRVTYQRGPYNELRGASTSSGQELRI
                     SSDDHLTEEQLSDQFGVITRRQLDLYLNPVESSDAEGPLGKAQYDLLGRLTRSERPDG
                     TARQFEYDPEGDLTRMTDARGGVWQARYTPYGDCVEQLDPLGRRHQFTWDTEGRLSSI
                     INPKSEVARFEYDLAGNLTAITHFDGTREQASYDLAARLIGRVRPDGTRLSFDRDDVG
                     NLVK"
     gene            complement(2051..3358)
                     /locus_tag="VFQ61_39705"
     CDS             complement(2051..3358)
                     /locus_tag="VFQ61_39705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960684.1"
                     /translation="MQWSPRLFLTASALCLIAGISSPSGLGCARPGVSSAPTHSASIT
                     PVSSAIAHQEHAPPVDPDLPAATRQVLGAFVEPELRTLMGPVSLRGELSALLKSLSGR
                     PTALAFLIDREVPEPLSRSLSWAIAAQRAAHVSFRAALVTWGVSGGLRAEVAFSDSFE
                     VMRAAGRAEWSPESGSMAASWTGLAALPGLAWPGPAEKHVVLLVDDREVGVSAGQPSN
                     RVIEKVMHWARTEQVRLHLWRCQLESEDGDVQSPEVRRPSRGLPLDVLAGLFRSGTWS
                     RTPAESLTEVVASAIKPWIESPGAVDLVLAVDTSGRTGESLSALYKMKSQMQEFLRGD
                     SRRMAVVDWDARKFRIAQPFSSDVEAQMRTLRALPRGPLGDRPKDLFGTIDRLRHRLE
                     WRSDSERAIIIVTSAAARLPVPDALLDWSDRERVSVSLIEVTN"
     gene            3482..>3872
                     /locus_tag="VFQ61_39710"
     CDS             3482..>3872
                     /locus_tag="VFQ61_39710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET9960685.1"
                     /translation="MAFAEQAVYTILNPKLLAEWASSTDDAQLRETKRWVTAKRYFDD
                     AQRRGLEMAVIYADAAQDCSKLIYWGRLTSVEVDADGTSYTVAGLTPLTGHRTQELVL
                     LSTSEQIAPGYIRPYAVVRRPPFLVQTT"
BASE COUNT          714 a         1222 c         1237 g          699 t
ORIGIN      
        1 cttgacgagg ttgccgacgt cgtcgcggtc gaagctgagg cgggtgccgt cagggcgcac
       61 gcgaccgatt aggcgcgcgg ctaagtcgta gctggcttgt tcgcgggtgc cgtcgaagtg
      121 ggtgatggcg gtgaggttgc cagcaaggtc gtattcgaag cgggcgactt cactctttgg
      181 gttgatgatg gagctgagtc ggccttcagt gtcccaggtg aattggtggc ggcggccgag
      241 tggatcgagc tgctcgacgc agtcgccgta aggcgtgtag cgcgcttgcc aaacgccacc
      301 gcgggcgtcg gtcatgcggg tgaggtcacc ttcggggtcg tattcgaatt ggcgcgcggt
      361 gccgtcgggg cgctcgctgc gggtcaggcg acccagcaag tcgtactggg ctttgccgag
      421 cgggccctcg gcgtcgctgc tctcgacggg gttgagatag agatcgagtt ggcggcgcgt
      481 gatgacgccg aattgatcgc tgagttgctc ctcggtgagg tggtcgtcgg aactgatgcg
      541 gagttcttgg ccgctgctgg tggaggcgcc gcgcagctcg ttgtaggggc cgcgctggta
      601 ggtgacgcgg cgctcgaacg gatctttgac cgcgaccagc gcgccgcgtt cgtaaccaaa
      661 ctcccacacg gctccgtcgg gctcggtgat gcgcttcggt tggtggtact cgtcgtattc
      721 gtattgaagc tttgcgccgt tcgggaagat cttctccgcg acgcggccca cgtcgtcata
      781 gacgtagcga gtggctttgc cgagcgggtc aatctcaccg acgatgcgcc cgtagtcatc
      841 gagctcagtc acatactgag cgccattggg caggatcttg tggtgaactt ggccctgggc
      901 gttgtactgg tagatcgtgt cgaagccgag gctatcggtg acgcgcgtgg tgcgccccgg
      961 ctcatagtga aagacgcact tgcggtagcc gccttcaccg ctcgtctcga tgcagcgccc
     1021 ctgggcgtca tagcgcatgc ggaagacgcc gccgcgccgt cccgtctccg cgatcagccg
     1081 gtgctcgtta tcatatgcgt aacgttgtgc cgctccgtgc gcgtcgaaca ccgcgatgag
     1141 ccgggattga gcatcgtatt cataacgggc caccagccgc gcttcccgcg ctgtctgagc
     1201 tacgagcccg aggtgcaagg tgctgagtcg gccctggttg tcataagcga gatgcagccg
     1261 gcggcgctct tgggtctggg tgatggcggt cgcgcgcccg agtcggtccc gctcgatgcg
     1321 caagccctgt ccggacggga gcacgcgcgc ggcgagccac atgaagtcgc cgtcccgcgt
     1381 gttgaagaca tacttttgga ccggctcgtc gacgctgtgc cagtgataga tgacgagctg
     1441 ctcgccctcc cggcgcagct ccatacaagc cgcgcgatcg atgagcgatg ccccctctcg
     1501 accactcgaa aaggtgccgt ccacgtcgtc gaactcgacg cggtcgccat cgtgaccttc
     1561 gaagacgtat ccatcgaggt cccgcttgag actggcctcg aaggcgtgaa cccagcctgg
     1621 gccgagcacc gtcggggcac gcttcaggaa cgccgtgttg tacacgcgtc ggaagatcag
     1681 cggcgaagtc ccgggtacct ccacgtcgtg ggcggccgtg aattgctcgc cggtcgcgac
     1741 atcgacgggg tgacccgcga acgctcgtgc tgactgggga cgagcggact ctgactcgcc
     1801 cgattgtgtc cgcgctgcct ccacccgcgc agcgcacggc gagctttcgg tcggttcggg
     1861 attcttacct cggtcggtgc tcacggtggc cttgttgtat cgcaacgagg aggaggcggg
     1921 ctcgagaaaa atcacctcgg cgatcgagcg cttgacaggt aacctattca ggtctgttgg
     1981 cgagccgtgg tcttctaggg tggcatgctc cccggcgaca tacctgaaga ctgacgagaa
     2041 tagctgacgc tcagttcgtc acttcaatca aagaaaccga aacacgctca cggtcgctcc
     2101 aatccagcag ggcgtctggc acagggagcc tcgcggcggc ggatgttacg atgatgatcg
     2161 cgcgctctga atccgagcgc cactcgaggc gatgacgaag gcgatcgatc gtgccaaaga
     2221 gatccttcgg cctatctccc aaggggcccc gcgggagtgc gcgcagcgtt cgcatctgtg
     2281 cctcgacgtc actactaaaa ggctgagcga ttcggaactt gcgggcgtcc cagtccacca
     2341 cggccatacg tcggctatcc ccacgcagaa attcctgcat ctgagacttc attttgtaga
     2401 gggccgacaa ggattctccc gtacgtcccg aagtgtcgac cgcaaggaca agatccacgg
     2461 cgcctgggct ctctatccac ggcttaatgg cactagcgac cacttcggtc agcgactccg
     2521 ctggcgtacg agaccacgtg ccacttcgga acagtcccgc cagcacatcg agcggcagac
     2581 cgcgtgacgg gcgcctgacc tcgggcgact ggacgtcacc gtcttccgac tcgagctggc
     2641 agcgccacaa atgaagccga acttgctccg tgcgcgccca atgcatcacc ttctcaatta
     2701 ccctgttcga cggctgaccg gcactgacgc cgacctcccg atcatcaacc aacagcacca
     2761 catgtttctc cgccggtcct ggccaagcca agcccggcaa agcagccaac cccgtccaac
     2821 tcgcggccat gcttccggac tccggtgacc actccgcgcg cccagcagcg cgcatcacct
     2881 cgaacgagtc ggaaaaggcc acctccgcgc gcaggccgcc actcacaccc cacgtgacca
     2941 gcgccgcgcg aaacgacaca tgcgcagccc tttgcgccgc gattgcccag ctcaacgagc
     3001 gcgacagcgg ttcgggcacc tcccggtcga tcaaaaacgc gagcgccgtg gggcgacctg
     3061 aaagcgattt caaaagcgcg cttaactcac cccgtaagga caccggaccc atcaatgtcc
     3121 ggagctccgg ttcaacgaaa gctcccaaca cctgtcgagt agcggcgggc aagtccgggt
     3181 cgaccggggg cgcgtgctcc tggtgagcga tggcagaaga gacaggcgtg attgaggcag
     3241 agtgcgtcgg ggcgctactg acccccggtc gcgcacagcc caacccgctc gggctcgaaa
     3301 tgcccgcaat gaggcagagc gccgaggcag tcagaaacag gcgaggggac cactgcatgg
     3361 agttagccta cgctgcgcga ctcccaaagc acatcggaac gttgggctcg caagtatcat
     3421 gcaagctggc gcattgaccg acgccactaa acagtgggtg tcccaggacg aataggaccc
     3481 aatggccttt gccgaacaag ctgtctacac catcctgaat ccgaagctcc tggcggaatg
     3541 ggcctccagc acggatgacg cgcagctgcg ggaaacgaaa cgctgggtga cggcgaagag
     3601 gtacttcgat gacgcgcaga gacgcggcct cgagatggcc gtgatctacg cggatgcggc
     3661 gcaggactgc tcaaagctaa tctactgggg ccgactcacc tccgttgaag tcgacgcaga
     3721 cggcacgtcg tacacggttg ccggcctgac gccgcttacc gggcaccgca cgcaggagtt
     3781 ggttctgctt tcgactagtg agcagattgc gccgggctac atccgaccct acgccgtcgt
     3841 tcgtcgaccg ccgttcctgg ttcaaactac cg
//
